LOCUS       DASRJB010000001         6409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_282291, whole genome shotgun sequence.
ACCESSION   DASRJB010000001 DASRJB010000000
VERSION     DASRJB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282291"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFB80_00005"
     CDS             complement(<1..292)
                     /locus_tag="VFB80_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN32156.1"
                     /translation="MSRVLIICGVLVALVLARLQAEEHRAGKTVSGVVRDSAGKPVEG
                     AEVWLVMTDMWFFESDVPSSTKTSAAGTFELAVPQRWLDAIPQVRQELGFVAH"
     gene            444..1712
                     /locus_tag="VFB80_00010"
     CDS             444..1712
                     /locus_tag="VFB80_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32157.1"
                     /translation="MSTRFACAILACAIIGQFPLRAQEQPAAAWNYVVPTAGEPMEHP
                     PLVPLVLFDKRPELVTNEPAYRSAKPLYGLLRYGSDTSPQVVVVLDERSVSGGGLRDG
                     GELDLYVDAARDPEITARQIAAGEGRLRRAAIKSEIAYLDRVAEEFPRQVLFRRGALG
                     SSLGVATLGYLEGQVTLNGKPVAARRVDGDANGLFSDPRDRLWLDLNADGQWDAFSEQ
                     FPVQPVMLLAGKRYAVRTDQAGTRLALEEVTGVGTLKLQLRSLAAGATIRSLEVALMG
                     DDGSAYAARADGAAMTVPAGKYAMRSLRVSLLPADSSKPWTFQFTRYEPPDEGEWQRI
                     GPGEEVTIDPCGQLRLAVEGDELKRPVKPGQMLTIHPRLYTAGGLLINSCDFHEKPAE
                     LGDSSHQNSCETSLCQTDGTAVASYSSGFA"
     gene            complement(1845..3212)
                     /locus_tag="VFB80_00015"
     CDS             complement(1845..3212)
                     /locus_tag="VFB80_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HZN32158.1"
                     /translation="MRSTCTFGLALAGWLLLTAVLAAQQATEPARPAPRATSPSPAAR
                     TPAAQALPADVIFEGGDVNYMLGHECGPGGCNFCGNFGGSDCCCCLSGVYARLEYLQW
                     GTRGMNVPPLVTTSPQGTPRENAGVLGEEGTEILFGGDTSGSNLRPGGRITFGRWFDP
                     CQRLGIEADYFALRDERTTFQRTSDGDPILARPFYDVTTGEESSELVAFPNVIRGTIS
                     VEHVSRFQGAGVRGIWNIACGDGCGTSCITHCPVPTGYRFDVLLGYRFLRLDDEVNIV
                     EDLVATGTEVQTGAFIVHDRFQTENQFHGVDFGSSLRFCKGCFTLDLLSKLALGNTRS
                     RIRIRGSTVITQNGQSESFEGGLLAQRTNIGDYTFNEFAAVPELGATLGYQINPCWRL
                     TFGYSFIYWSRLVRAGDVINLDVNPNLLPPEDPLVTTHLRPDLRFCYSDFWAQGMNFG
                     IEGKW"
     gene            complement(3374..3658)
                     /locus_tag="VFB80_00020"
     CDS             complement(3374..3658)
                     /locus_tag="VFB80_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32159.1"
                     /translation="MIGVQLFNALEAAFWFVLAILAATRGRQVRGFTPRRQLALCFFL
                     AAFGASDLWEIFSGGWWKPPALLALKAVCFGGLAVSAALIYGTRWRSVKS"
     gene            complement(3698..4561)
                     /gene="truB"
                     /locus_tag="VFB80_00025"
     CDS             complement(3698..4561)
                     /gene="truB"
                     /locus_tag="VFB80_00025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZN32160.1"
                     /translation="MFGFLNIDKPGGVTSRDVVNRVQRLVRPHKVGHAGTLDPLATGV
                     LVVAVGPATRLIEYVQRLPKTYLATFLLGRSSDTEDIAGEVVELPGAAVPTETQLRAA
                     LPQFLGTIQQRPPAFSALKVAGQRAYDLARRGDAVELLPRPVEIYDLEILRYAYPELQ
                     LLIRCGSGTYVRSLGRDLAESLGTAAVMSALRRLVIGPFTAETAIAADSLSASSIEHQ
                     LLSPLMALGEMPRIEVNEAASLRLAQGQAIENRWNVSSAEVAAVNSAGQLVAILSPLA
                     ERLRPQRCFHC"
     gene            complement(4575..6122)
                     /locus_tag="VFB80_00030"
     CDS             complement(4575..6122)
                     /locus_tag="VFB80_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN32161.1"
                     /translation="MSAQYIKLSRRENQALGIGRYAIDFVGGRLGQPAASVLAKVEQF
                     HTDSVGCAIAALAIGANAPVLLRQEALAYPRPGGVCCFGSSERVAPEKAVLANSAAVR
                     ELDANGTNFGYNPRTGQKRGEFGHNDFYPVALAAAQLAGCDGRRTLLAMLCLDEIRGR
                     LAEAFGLKDHKIDHVLHGAIASAAVYGAVLGATAEQIESAIGLVVSHYVPFRAIRHGE
                     QLSDSKGASAAISAEVAVLSMWRAMRGFAGPADIFRNPQALFCLFERPAEKGASPFDL
                     ELTAGGDDFAVMGMHFKLGLYEHQSAGAIGGLLDLLARQPQLAADPAAIERIRITIYE
                     PAFSIIGDPHKRDPRTRQSADHSMVYIVATLLRKAVESGVGSRASGVGAVDFWRELML
                     GPEDYSEAALAHPLTRALMQRIDFRHGGPEYDAKYPDGIPTSLEIDHAALGTLASGLV
                     MYPEGHARNTSGHLPALLAHKFRRLAGLGVADMDSLLARMSNLAAKSPAEIRELYSIP
                     VTVLQRA"
     gene            6179..>6409
                     /locus_tag="VFB80_00035"
     CDS             6179..>6409
                     /locus_tag="VFB80_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZN32162.1"
                     /translation="MDACSGFPTTRLRRLRYHPAVRSLVRETRLSPASLILPLFARPG
                     KAVRQPIASMPGHAQLSVDQIAEEALLAKKLGL"
BASE COUNT         1125 a         2155 c         2111 g         1018 t
ORIGIN      
        1 ggtgcgcgac gaagcccagc tcttgccgca cttgggggat ggcgtcgagc cagcgctgcg
       61 gcacggccaa ttcgaacgtc cccgcggccg acgttttcgt tgagcttgga acatcgcttt
      121 cgaagaacca catatcggtc atgaccagcc aaacttccgc gccctccacc ggcttgccgg
      181 cagagtcgcg gaccacgcca ctcacggtct tgccagcccg atgttcctcc gcttgcagcc
      241 gcgccagaac caatgccacc agcacgccgc agatgatgag tacgcgggac atgagaatgt
      301 ctcccagcgg tccgagataa gacagtccgc ataaattcgt ccgaccatgt ccaatattgg
      361 tgttgccaag agcgaatgta agacataatg cctaaaagtt gcagcggtcg ttttcttgtc
      421 gcgcgaagct gacggaggga ccgatgtcca cgcgtttcgc ctgcgctatt ctcgcctgtg
      481 cgatcatcgg acagttcccc ctgcgagctc aggagcagcc cgccgccgcc tggaactacg
      541 tcgtccccac ggcgggcgag ccaatggagc atccgccgct ggtgccgctc gtgctgttcg
      601 ataagcggcc ggagctcgtg accaacgagc cggcgtaccg cagtgccaag ccgttgtatg
      661 gtctgctgcg ctacggctcg gacacttcgc cgcaagtcgt ggtcgtgctc gacgaacgca
      721 gtgtttctgg cggcgggctg cgcgacggcg gcgagctcga cctttatgtc gatgccgctc
      781 gcgatccgga gatcactgcg cggcaaatcg cggcgggcga gggccggctg cggcgggcag
      841 cgatcaagag cgagatcgcc tacctcgatc gcgttgccga agaatttccg cggcaggtcc
      901 tcttccgccg cggcgcactg ggctcctcgc tcggtgtggc gacgctcggc tatctggagg
      961 gccaggtcac gctcaacggc aagccggtgg cggcccgccg cgtcgatggc gatgccaacg
     1021 ggctgttttc cgaccctcgc gaccggctgt ggctcgacct caatgccgac ggccagtggg
     1081 acgcgttcag cgagcagttt cccgtgcagc cggtgatgct gctggcgggc aagcggtacg
     1141 ccgtccgcac cgaccaggcc ggcacgcggc tggcgctgga agaagtgacc ggcgtcggca
     1201 cgctcaaact gcagctccgg tcgctggctg ccggcgcgac gattcgcagc cttgaagtcg
     1261 cgctgatggg cgacgacgga tcggcttacg ccgcgcgggc cgacggggcg gcgatgactg
     1321 ttcccgcggg caaatatgcc atgcgttcgc tccgcgtgtc gctgctgcct gccgattcgt
     1381 ctaaaccgtg gacctttcag ttcacgcgct atgagccgcc ggacgagggc gagtggcagc
     1441 ggattggccc gggcgaggag gtgacgatcg atccctgcgg acagctgcgg ctggccgtgg
     1501 aaggcgacga gttgaagcgg cccgtaaagc cgggacagat gctgaccatc catccgcggc
     1561 tgtacacggc cggcggattg ctgatcaact cctgcgactt ccacgagaag ccggccgagc
     1621 tgggcgacag cagccatcag aactcgtgcg agaccagcct ctgccagacc gacggcacgg
     1681 cagtcgccag ctacagcagc ggctttgcct gaggcacgtt ctgcccggcc tcggtccgag
     1741 tgcccaccaa tcaggccccc ggtcaaatgc tggtccgcat cacgttcgac gccggcccgc
     1801 tcggcggtgc gatgcaagtc gagcgcagaa tcgagattca gtaactacca cttgccttcg
     1861 atgccaaagt tcattccctg ggcccagaaa tcggagtagc agaatctcag atcgggccgc
     1921 aggtgcgtcg tcaccagggg gtcttccggc ggcagcaggt tgggattcac gtcgaggttg
     1981 atcacgtcgc cggctcgcac caggcggctc cagtagatga acgaatagcc gaacgtcagc
     2041 cgccagcagg gattgatctg gtagccgagc gtcgcgccca gttcgggcac ggcggcgaac
     2101 tcgttgaagg tgtagtcgcc gatgttcgtc cgctgggcga gcaacccgcc ctcgaacgat
     2161 tcggactggc cgttctgcgt gatgacggtg ctgccgcgaa tgcggatgcg gctgcgggtg
     2221 ttgcccagcg ccagcttcga gagcaggtcg agcgtgaaac agcccttgca gaaccgcagc
     2281 gagctgccga agtccacgcc gtgaaattgg ttctcggtct gaaagcggtc gtggacgatg
     2341 aaggcgccgg tctgcacttc agtgccggtc gcgacgaggt cctcgacgat gttgacctcg
     2401 tcgtccagcc gcaggaagcg atagcccaac agcacgtcga agcgatagcc cgtgggaacg
     2461 gggcaatggg tgatgcagct ggtgccgcag ccgtcgccgc aggcgatgtt ccaaatgccg
     2521 cgcacgcccg cgccctggaa gcgcgagacg tgctcgaccg aaatcgtgcc gcgaatgaca
     2581 ttgggaaacg ccaccagctc ggagctctct tcgcccgtcg tcacgtcgta aaacggccgg
     2641 gccaggatcg ggtcgccgtc gctggtgcgt tgaaaagtcg tgcgttcgtc gcgcagcgcg
     2701 aagtagtcgg cctcgatgcc gagccgctgg caaggatcga accagcggcc gaacgtgatc
     2761 cgcccgccgg ggcggaggtt gctgcctgac gtgtctccgc cgaagaggat ttccgtcccc
     2821 tcctcaccaa gcactccagc gttctcccgc ggagtgccct gggggctcgt cgtgacaagc
     2881 ggcggcacgt tcatcccgcg cgtgccccac tgcaggtatt cgaggcgagc atacaccccg
     2941 ctcaggcaac agcagcaatc ggagccgccg aagtttccgc aaaagttgca gccgcccggg
     3001 ccgcactcgt ggcccagcat gtagttgacg tcgccgcctt cgaaaatgac atccgcgggc
     3061 agagcctggg ccgccggcgt cctcgcggcg gggctcggac tggtggcgcg cggcgcgggc
     3121 cgcgcaggct ccgtcgcctg ctgggcggcc aggaccgcgg tcagcagcag ccagccagcc
     3181 agcgcaagac caaaggtgca ggtggagcgc acgatccaac tccctccctg gaggtgaccc
     3241 gcggctgtgc tccaaaacat tcccgctgag cgcagccggg gcgcgtcgat gatgagatca
     3301 gcgtgggctt gtagtcgaat cggcaatccc cgaaaagggc aattcggccg gccgccagca
     3361 gcctcagtcg tcgctagctc ttcacggagc gccagcgtgt gccgtagatg agtgcggcgg
     3421 aaacggccag gccgccaaag caaacggctt tgagagccaa tagcgccggc ggcttccacc
     3481 agccgccgga gaagatctcc cacaggtcgc tcgcgccgaa cgccgccagg aagaagcaca
     3541 gcgccagctg ccggcggggc gtgaagcccc ggacctgccg gccgcgggtc gccgccagga
     3601 tcgccagcac gaaccagaac gccgcctcca aagcgttgaa cagctgcacg ccgatcatgc
     3661 gtattcaaca cgcgggctgc gatcatggat cggtcggtca gcagtgaaag caccgctggg
     3721 gccgcagcct ttcggccagc ggcgagagga tcgccaccag ctggcctgcc gagttcaccg
     3781 cggcgacttc cgcggaggaa acgttccagc gattctcaat ggcctggccc tgggcgaggc
     3841 ggagactcgc ggcctcgttc acttcgattc gcggcatttc gccgagagcc atgaggggcg
     3901 agagcaattg gtgctcgata ctcgacgctg agaggctgtc ggcagcgatg gcggtctcgg
     3961 cggtgaacgg gccgatgacc agccggcgga gggccgacat gacggcggcc gtgccgagcg
     4021 actcggccag gtcgcggccc agcgagcgga catacgtccc gctgccgcag cgaatgagca
     4081 gctggagctc aggataggca tagcgcagga tttccaggtc gtagatttcg acgggccggg
     4141 gcaaaagctc gacggcgtct ccgcggcggg ccaggtcgta cgcccgctgg ccggcgacct
     4201 tcagcgcgga aaaggccggc ggccgctgct ggatcgtgcc caaaaactgc ggcagcgccg
     4261 cgcgaagctg tgtttccgtc ggcacggcgg cgccgggcag ctccactacc tcgcccgcga
     4321 tgtcctcggt gtcgctgctg cggcccagca ggaacgtcgc caggtacgtc ttcggcaagc
     4381 gctgcacgta ctcgatcagc cgtgtggcgg gacccaccgc caccacgagc acaccggtcg
     4441 ccagcgggtc gagtgtgccg gcgtgaccga ccttgtgcgg acggaccagc cgctggacgc
     4501 ggttgacgac gtcccgcgac gtgacgccgc ccggcttgtc gatgttcaag aacccgaaca
     4561 agaaacctcc ggccctaagc ccgctgcagg acagtgacgg gaatcgaata cagctcgcga
     4621 atctccgccg gagacttagc ggcaagattc gacattcgcg cgagcaggct atccatgtcg
     4681 gcgacgccca gcccggccag ccgccggaac ttgtgggcca agagcgccgg caagtggccg
     4741 ctcgtgttgc gggcgtgccc ttcgggatac atcaccagtc ccgacgccag tgtgcccagc
     4801 gctgcatggt cgatctccag cgatgtcgga atcccgtcgg ggtacttcgc gtcgtattcc
     4861 ggcccgccat gccggaaatc gatccgctgc atcagcgccc gcgtcagcgg atgcgccagc
     4921 gccgcctcgg aataatcctc cggccccagc atcaactcgc gccaaaaatc caccgccccg
     4981 actcccgacg cccgactccc gacgcccgat tccacggcct tccgcagcag cgtcgccaca
     5041 atatagacca tcgaatgatc cgcgctctgc cgcgtccgcg ggtcgcgctt gtgcgggtcg
     5101 ccgataatgc tgaacgccgg ttcgtagatc gtgatccgga ttcgctcgat cgccgcggga
     5161 tcggcggcca gctgcggctg cctcgcgagc aggtccagta gcccgccgat cgcgccagcc
     5221 gactgatgct cgtagaggcc gagcttgaag tgcatgccca tgacggcgaa atcgtcgccg
     5281 ccggcagtca gttccaggtc gaagggactc gcgccctttt ccgccggccg ctcgaacagg
     5341 caaaacaacg cctgcgggtt gcggaagata tcggccgggc cggcgaatcc gcgcatcgct
     5401 cgccacatcg agaggaccgc cacctccgca ctgatcgccg ccgaggcgcc cttgctgtcc
     5461 gagagctgct cgccgtggcg gatcgcccgg aacggaacat agtgggatac caccagccca
     5521 atcgccgatt caatctgctc cgccgtcgcc ccgagcactg cgccatacac ggccgccgaa
     5581 gcaatcgccc cgtggagcac gtgatcgatc ttgtggtcct tgaggccgaa cgcttcggcc
     5641 agccgcccgc gaatctcatc gaggcaaagc atcgccaaga gcgtgcggcg gccgtcgcag
     5701 ccggcgagct gcgcggcggc cagcgccacg gggtagaagt cgttgtggcc gaattcgccg
     5761 cgcttttggc ccgtccgcgg gttgtagcca aaattcgtgc cgttggcgtc cagctcgcgg
     5821 accgccgcgg agttcgccag cactgccttc tcgggcgcga cccgctcgct gctgccaaag
     5881 cagcagacgc cgccgggacg ggggtacgcc agcgcttcct gccgcagcag cacgggagca
     5941 ttcgcgccga tcgccagtgc cgcaatggca cagccgacgc tgtccgtgtg gaattgctcg
     6001 accttggcca acaccgacgc ggccggctgc cccaggcggc caccgacaaa gtcgatcgcg
     6061 tatcggccga tgccgagggc ctggttctcg cgccgcgata gtttgatgta ttgcgccgac
     6121 atgcctgatt cgagcgttgc ccgttcgcgc caagggagaa cgcaattata aaggaggaat
     6181 ggacgcctgt tccggctttc ccacgacccg cctccgccgg ctgcggtatc acccggccgt
     6241 gcggagcctc gtccgcgaga cgcggctctc gccagccagc ctgatcctgc cgctctttgc
     6301 ccggccgggc aaagccgtgc ggcagccgat cgcctcgatg cccggccacg cgcagctgtc
     6361 ggtcgatcag atcgcggagg aggcgctgct ggcaaaaaaa ctcggcctg
//
LOCUS       DASRJB010000002         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3104074, whole genome shotgun sequence.
ACCESSION   DASRJB010000002 DASRJB010000000
VERSION     DASRJB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104074"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFB80_00040"
     CDS             complement(<1..334)
                     /locus_tag="VFB80_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN32163.1"
                     /translation="MDPQRERIQADLRGLLSGEVFCDDPFTLMYASDASIYQIPPLGV
                     VRPRGLADVQACLKYAAEQSLPIHARGAGTGLAGESLGSGLVLDFSRSMRRIVSVNGD
                     TVTVQPGVV"
     gene            complement(341..817)
                     /locus_tag="VFB80_00045"
     CDS             complement(341..817)
                     /locus_tag="VFB80_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32164.1"
                     /translation="MNVTFRCPQCDQLGCVPLEEAREIACPACQAHWPLPAGAVADGQ
                     VSRCVICPSTELYVRKDFSQALGIAIVVVGFVISSVFWYWRMPMWTYAVLFATALIDI
                     VLYLTVGNLLQCYRCQAQYRGLTGLESHQPFDLETHEKHRQQKIRLAQASSPKSAI"
     gene            complement(841..1638)
                     /locus_tag="VFB80_00050"
     CDS             complement(841..1638)
                     /locus_tag="VFB80_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32165.1"
                     /translation="MFGHILVEFLFRLTCGVAAAMGCTNSREVTSGFFRVHLWVLLGV
                     QTLAALAIYSGRPTGTSPRANLIWQGQLGLAIAAAVACYLSAVIWMYERRIAGKLGVW
                     LVALLAALALSLPVTFGRSKEVVMQIADRATGSALLGFVTTAMLLGHWYLNTPTMKLA
                     PLRRLIVLLAIAALGRMVVCAAGGWLEFASHSFASASARQSWLLFLALRWLAGLVGVL
                     GLAGLTWQTLKIPNTQSATGILYAAVILVFIGELTSQLLSAEAHYPI"
     gene            complement(1719..>2117)
                     /gene="folE"
                     /locus_tag="VFB80_00055"
     CDS             complement(1719..>2117)
                     /gene="folE"
                     /locus_tag="VFB80_00055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZN32166.1"
                     /translation="FSEQYDEVVLVRDISFCSMCEHHLLPFIGTAHVAYIPNGKVIGL
                     SKIARVVEVISRRPQVQERMTEEIANLLEGELDARGVAVVIEASHSCMTIRGVKKPGS
                     SMVTSAMKGVFRSNVSSRAEVMQLIYGKSR"
BASE COUNT          415 a          686 c          682 g          334 t
ORIGIN      
        1 gcacgacgcc cggctgcacc gtgaccgtat cgccattgac gctgacgatc cgccgcatcg
       61 agcggctgaa atcgagcacc aggcccgatc cgagcgattc accggccagc cccgtgcccg
      121 cgccgcgggc gtggatcggc agcgactgtt ccgcggcgta tttcaggcag gcctgcacgt
      181 cggccagacc ccgcggccgc acaacgccca gcggcgggat ctgatagatg ctggcgtcgg
      241 acgcgtacat cagcgtgaac ggatcgtcgc aaaagacctc gcccgagagc aggccccgca
      301 ggtcggcttg aatccgctcg cgctggggat ccatggaaac tcagattgca gatttggggg
      361 acgaagcctg cgcgaggcgg attttttgtt ggcggtgttt ttcgtgcgtt tcgaggtcga
      421 agggctggtg gctttccagg ccggtcaggc cgcggtactg ggcctggcag cgatagcact
      481 gcagcaggtt gccgaccgtg agatagagca cgatgtcgat cagggccgtg gcgaagagca
      541 cggcgtaggt ccacatcggc atccgccaat accagaagac cgagctgatg acgaagccaa
      601 cgacgacgat cgcgatcccc agcgcctgcg aaaaatcctt gcggacgtag agctcggtgc
      661 tggggcagat gacgcagcgg gaaacttgcc cgtcggcgac cgcgcccgcg ggcagcggcc
      721 aatgcgcctg gcacgcgggg caggcgattt cgcgcgcttc ctccagcggc acgcagccga
      781 gctggtcgca ctgcggacag cgaaacgtaa cgttcatcgt ttcatcctac tccaggcggt
      841 tcagatcgga taatgggctt ccgccgaaag cagttgcgag gtcaactcgc cgatgaagac
      901 cagaatcacc gcagcgtaga ggatgcccgt cgcactctgc gtgttgggaa tcttgagcgt
      961 ctgccaggtc agccccgcca gccccagcac accgaccaga ccggccagcc agcgcagcgc
     1021 caggaacagc agccaggact ggcgggccga ggcggaggca aacgagtgcg atgcgaattc
     1081 cagccagcca cccgcggcgc acacgaccat gcggccgagc gcggcaatgg ccaacaggac
     1141 aatcagccgc cgcagcggcg ccagcttcat cgtgggcgtg ttgagatacc agtgtcccag
     1201 cagcatcgcg gtcgtgacaa agccgagcag cgcgctgccg gttgcgcggt cggcaatctg
     1261 catgacgact tccttggatc tcccaaaagt caccgggagc gatagtgcaa gggccgccag
     1321 cagggcgact agccaaacgc ccagcttgcc ggcgatccgg cgctcgtaca tccagatgac
     1381 ggcggacagg tagcaggcga cggccgcggc aatcgccagc ccgagctgcc cttgccagat
     1441 gagattggcc cgcgggctgg ttcccgtcgg ccggccggaa tagatcgcca gcgcggccag
     1501 cgtttgcacg cccaagagca cccacaagtg aactcggaag aagccgctgg tcacttcccg
     1561 gctattcgtg cagcccatcg ccgccgcgac gccgcacgtc aggcggaaca gaaactcgac
     1621 gaggatatga ccaaacacgt gtttcttgta gggaacgccc tctgtggcgt tccgttttcc
     1681 cgcggcacgg aacgctacag ggggcgttcc ctacaggctc accgcgactt cccataaatc
     1741 agctgcatca cctcggcccg gctggagacg ttcgaccgga agacgccctt catggccgaa
     1801 gtgaccatgc tgctgccggg cttcttcacg ccgcggatcg tcatgcagct atgcgaggcc
     1861 tcgatcacga cggcgacgcc tctggcgtcg agctcgcctt ccagcaggtt ggcgatctct
     1921 tcggtcatcc gctcctgaac ctgcgggcgg cgcgagatga cctcgacgac gcgggcgatc
     1981 ttcgacaggc cgatcacctt gccgttggga atgtaagcca cgtgtgccgt gccgatgaac
     2041 ggcaacaggt gatgctcgca catgctgcaa aagctgatgt cgcggactag cacgacctcg
     2101 tcgtactgtt cggagaa
//
LOCUS       DASRJB010000003         8139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3386340, whole genome shotgun sequence.
ACCESSION   DASRJB010000003 DASRJB010000000
VERSION     DASRJB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386340"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFB80_00060"
     CDS             complement(<1..215)
                     /locus_tag="VFB80_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32167.1"
                     /translation="MTQDINVLALVKGEERYIFLFDDERRSEALRTLGRFASNPELSF
                     SWYDAAVLSQRIRQTAIEQRAQAQHPQP"
     gene            complement(286..1341)
                     /locus_tag="VFB80_00065"
     CDS             complement(286..1341)
                     /locus_tag="VFB80_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32168.1"
                     /translation="MTAAAHLRLALLGAALCLCTGCASWPDRVVEPQYHNPFPQLHRV
                     AVLPFFNQSAEPTVDGETVAIAYYNELQLIPGFEVMPVGVARQMLAASIAATGSEPRT
                     GSDFQRLAEQMGVDAVLVGSITEYSPYYPPRLGLAVDWYAANPGFHPIPSGYGLPWGR
                     AEEEYIPSALVREAEFALAREQLKTQTPDVPPREIPGAVAPAATSGARTESAQGAESA
                     HAARPAQPDAIASKLDESAALAAPHLPADWPDPRGFIPLPPSPARPVLRPQHAPLITH
                     TRMFNGHDSKFTEKLATFYYLRDDARFGGWPAYLERPEDFVRFCCHLHLTETLAARGG
                     AGDSQVLWRWPVRRYER"
     gene            1632..3944
                     /gene="eutB"
                     /locus_tag="VFB80_00070"
     CDS             1632..3944
                     /gene="eutB"
                     /locus_tag="VFB80_00070"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HZN32169.1"
                     /translation="MLRRRTLLKAALGAAAAAATPVASSAEQPVAARGVSLAAALPGE
                     DVFSYLRRTAGGFDDRRYKQILGAANPFKEGDQIVGVAAADAAARETARGLLAATRIE
                     QIDVHPPLEDKLFRLLQQSRDAQAAAAAARLTLGELKRLLLHEDEAAIRRLMPGLSSD
                     TIGCVVKLLSDEELIAASRKIFNPLPGSRIGAKGYLGARIQPNSPTDNVSDIQWQVLD
                     GWSYAVGDVVLGTNPVSSDPDSVAAIERTLGDLLATFGLDDVLPHCVLAHIDVQAAVE
                     KLLPGGTGIWFQSIAGSDAANATFDISLEKMLAYADGRTGRYGLYFETGQGADFTNGH
                     DHGYDMVLHESRKYGFARALAQRVAAAQRRAGSDLAPWLHVNDVAGFIGPEVFRTREQ
                     LVRCCLEDIVMGKLHGLCLGLDVCSTLHMDVSLDDLDWCLDRILPANPAYLMALPTKI
                     DPMLGYLTTGFQDHVRLREKFGYRVNDRMWRFFQELEVIDERGRPTSHFGDPLWVYLK
                     YRRKKGDSRGDDEILREGRQQVDAVRRRGVFVAEGHGQEPWDLKPELAREIVRIYEDS
                     KECLWAELEPAFIRAIPERVQLATRSSDRTDYILHPASGEKLSDESLQALGELRRRAL
                     PYDVQIVVSDGLNALSIMDEGHLAPFLTRLRAALKDGGLRPAPQNLVFTSGRVRAGYR
                     VGEALFGGMSGVRSIVHVIGERPGTGHHTFSAYITAAPGTAWATSGKIDHDITKVVSG
                     IASTAQLPAQAADETARLLLGLFARMSQHK"
     gene            4028..4990
                     /locus_tag="VFB80_00075"
     CDS             4028..4990
                     /locus_tag="VFB80_00075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN32170.1"
                     /translation="MVLFPLRRRSSATGNPRTVVQCYSLFEHFFPPCGLLDYTEGIYH
                     GDASLPIEVAQQNQLEYVLDEAGCTAGSRILEIGCGNGTLLEAATRRGAQAVGITISP
                     EQVARCRRRNLDARLLNYLELDVEWARRFDAVIANGPIEHFVQPREAAADRSDAIYRQ
                     LFDICHRMIDPRSSVRRLVTTTIHFIRPPEPANLLKSPLAFPRGSDNFHWAMLARSFG
                     GWYPVPGQFERCAAGRFSVVRMTDGTHDYHLTSEEWLRRIRAVLPTLKGCRLLLGAVP
                     FGLRHPVQCATMFACMLASESWNWQFRGPAPPTQLLRHTWAYSA"
     gene            5087..6001
                     /locus_tag="VFB80_00080"
     CDS             5087..6001
                     /locus_tag="VFB80_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN32171.1"
                     /translation="MPSPLLDSTNRILPALVTPLLSDGRLDEKSAERLIDHLYATGVG
                     GLYLTGTTGEGIYLDFDIRRRIVEIACRQSQGRGQVIVHVGAIQAAKSLELGAHAAKA
                     GADAVSSIPPFAGGYSFDEVRAFYAELAKQSPLPVVAYYIPALTGQQHSIANLAALLR
                     LPNVAGLKFTDPNFYTMQRLLTRLSAEQVLYNGQDEALALGLQVGAHGGIGTTYNFMP
                     ELILEIYQHCQAGRYAEALAAQRKANDIIEALLMFHVVAAAKQILYWQGLIDHPHCAF
                     PRALLTSAQQGQLRDALSTTSIAHTLVR"
     gene            6114..7553
                     /locus_tag="VFB80_00085"
     CDS             6114..7553
                     /locus_tag="VFB80_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32172.1"
                     /translation="MPLCAILALGVILVTVCTTTANGQSLKAGAATSNITPPIGAQIV
                     GGHLPVPSTHVHDELHARCLVLDDGRTKLALVTCDVVGVHRLVCEAAKELIQAEAGIP
                     PANVLISATHTHSAASVQGQDRFKHSESLDEYQKFVVRRIADGVRRAATNLRPAELGY
                     GTAQAPEHLFNRRWFLKPGTMPENPFGGIDQVKMNPGTSPNLVEPAGPTDPTISFFAV
                     REPGGRPISLYAAYGLHYVGGTGGGAISADYFAVFCDEIQRLLGADRLDPPFVALLAN
                     GTSGDVNNINFREPRPPQKPYEQIRYVAGDVAAKVHAAMEKVQYQTDVRLDARLREPT
                     LRWRRPSADELAAAKKRFAEGVKVARGADLPYLYAERAIKLAEYPETTTVPIQVLAIG
                     DLSIGTMPCEVFTETGLEWRQVSPIQPAFFVELANGSFGYLPTPRQHKLGGYETWLGT
                     NRLEIEASEKLLAELVAMAGEIKAARQAK"
     gene            7662..>8139
                     /locus_tag="VFB80_00090"
     CDS             7662..>8139
                     /locus_tag="VFB80_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN32173.1"
                     /translation="MRCFPLLILFGASVVAAQPPQARIAASHGYDKAVELMRGNARAV
                     LCPEIGGRVLEFSVGGKQAMWFDEAERTRQPGGPAPSSAGRFDFGPEMTTPRHMLIWG
                     GAWTAEITGPSSARLTSQKDASSGVQLMRDFELAESKAATSGRGPQIRLICKQTITN"
BASE COUNT         1395 a         2777 c         2599 g         1368 t
ORIGIN      
        1 ggctgcggat gctgcgcctg ggcccgctgc tcgatggcgg tctggcggat tcgctggctg
       61 aggacggcgg cgtcgtacca gctgaagctc aattcggggt tgctggcaaa gcggccgagc
      121 gtccgcaggg cctcgctccg ccgctcgtcg tcgaacagaa agatgtaccg ctcctcgccc
      181 ttgaccagcg ccagcacatt gatgtcttgc gtcacgaagc gtctcctggg tcggtcctgg
      241 gcatcctgcc ttggtggctc cgccggaggc ggcgcgcgtg cttccctacc gctcatatcg
      301 gcggacgggc cagcgccaca gcacctgcga gtctcccgcc ccccctcttg ccgccaacgt
      361 ttccgtcaga tgcaagtggc agcaaaaccg cacgaagtct tccggtcgtt ccagatacgc
      421 gggccagccg ccgaagcggg cgtcgtcgcg caggtagtag aacgttgcca gcttctcggt
      481 gaacttcgaa tcgtgcccgt tgaacatccg cgtgtgtgtg atgagcggcg cgtgctgcgg
      541 gcgcaaaacc ggacgcgcgg gcgacggcgg cagcgggatg aaaccccgcg gatcgggcca
      601 gtccgcaggc aaatgcggtg ctgccagcgc agcgctttcg tccagcttgc tcgcaatggc
      661 gtccggctgc gcgggtcgcg ccgcatgcgc cgattctgcg ccttgtgcgg attcggttcg
      721 cgcgccgctc gtggcagcgg gcgcgacagc gccgggaatt tcgcgcggcg gcacgtcggg
      781 cgtctgcgtc ttcaactgct cgcgggccag cgcgaactcg gcctcgcgca ccagcgcgct
      841 gggaatgtat tcctcctccg cgcggcccca cggcaggccg tagccggacg gtatcggatg
      901 aaagcccggg ttggccgcgt accaatcgac cgccagcccc agccgcggcg gatagtaggg
      961 cgagtattcc gtgatcgagc cgacgagcac cgcatcgacg cccatctgct cggccagacg
     1021 ctgaaagtcc gagccggtcc gcggctcgct gcctgtagcg gcgatgctgg cagccagcat
     1081 ctgcctggcc acgccgaccg gcatcacctc gaagccgggg atgagctgca attcgttgta
     1141 gtacgcaatc gcgaccgttt ccccatcgac cgtgggctcc gcgctctggt taaagaacgg
     1201 cagcacagcc acgcggtgca gctgcggaaa gggattgtgg tattgcggct ctacgacgcg
     1261 atccggccac gaagcgcagc cggtgcacaa gcacagcgcg gcgccaagga gcgccaggcg
     1321 aaggtgagca gccgccgtca tggatttggg ggtagaacga tctccgccag gcccgctagc
     1381 ggcacggctg caatcgcagc ggcgcgcaag acagcccgcg gcgtccccct tatcggcaga
     1441 ctcaccgcag attctccaga cagaatcaac cgagaatgta gccgaactcg ccagagttcg
     1501 gatggatgcc ttaatcaccg aattctggcg aattcggcta cccaaagcat ttcgcgtcag
     1561 cagctagcca acgcggaacg acgcggagca caatgggtgc ttgcggttcg accgtcgccg
     1621 ggagtgacgc catgctacgc cgccgcacgc ttttgaaagc cgcactcggc gcggccgcgg
     1681 ccgctgcaac gcccgtcgct tcgtcggccg agcaaccggt ggcggcgcgc ggcgtttcgc
     1741 tggcggccgc gctgccgggg gaagatgtct tttcgtatct gcgtcgcacg gcgggcggtt
     1801 tcgacgaccg ccgctacaag caaatcctcg gtgcggccaa tccgttcaag gaaggggatc
     1861 agattgtcgg cgttgcggcg gcagatgcgg ctgcgcgcga aacggcccgc gggctcttgg
     1921 cggccacgcg catcgagcag atcgacgtcc acccgccgct ggaagacaag ctctttcgcc
     1981 tgctccagca gagccgtgac gcacaagctg cggcagcggc tgcaaggctc acgctcggcg
     2041 aattgaagcg gctgctgctg cacgaggacg aagccgccat ccgccgcctc atgccgggac
     2101 tgtcgagcga tacaatcggc tgcgtcgtca agctcttgag cgacgaagag ctgatcgccg
     2161 ccagccgcaa gatcttcaac ccactgcccg gcagccggat cggcgctaaa ggctacctcg
     2221 gggcacgcat ccagccgaat tcgccgaccg ataacgtcag cgacatccag tggcaggtgc
     2281 tcgacggctg gtcgtacgcc gtcggcgacg tcgtgctggg aacgaacccc gtttcgagcg
     2341 acccggattc ggtggccgcg atcgagcgga cgctcgggga cctgctggca acatttggcc
     2401 tggacgacgt cctgccgcat tgcgtgctgg cccacatcga cgtgcaggcc gccgtggaga
     2461 agctgctgcc cggcggaacc ggcatctggt tccagagcat cgccggcagc gacgcggcca
     2521 acgcaacctt cgatatttcg ctggagaaga tgctggccta cgccgacggc cgcaccggcc
     2581 gctacggctt gtacttcgaa acgggccagg gcgcggactt caccaacggc cacgatcacg
     2641 gctacgacat ggtcctgcac gagtcgcgga agtacggctt cgcgcgggcc ctggcgcagc
     2701 gcgtggccgc ggcccagcgg cgggccggca gcgacctggc cccttggctg cacgtcaacg
     2761 acgtcgccgg gttcatcggg ccggaggtct ttcgcacgcg cgagcaactc gtccgctgct
     2821 gcctggaaga catcgtcatg ggcaagctgc acggcctgtg cctggggctc gatgtctgct
     2881 cgaccctgca catggacgtc tcactcgacg atctggattg gtgcctggat cggatactgc
     2941 cggccaaccc ggcctacttg atggccttgc ccacgaagat cgacccgatg ctgggctatc
     3001 tcacgacggg gtttcaggac cacgtacggc tgcgagagaa gttcggctat cgcgtgaacg
     3061 accggatgtg gcggttcttc caggagctgg aggtcatcga cgagcgcggc cggccgacaa
     3121 gccatttcgg cgatccgttg tgggtctatc tcaagtatcg ccgcaagaag ggggatagcc
     3181 gcggtgacga tgaaatcctc cgcgagggcc gccagcaagt ggacgccgtg cggcgccgcg
     3241 gcgtctttgt cgcagaggga cacggccagg agccgtggga tctcaagccg gagcttgccc
     3301 gcgagatcgt tcgaatctac gaggattcca aagagtgctt gtgggccgag ttggagccag
     3361 cgttcatcag ggcgatcccg gagcgcgtgc agctggcaac ccgctcgtcc gatcggaccg
     3421 actacattct gcatcccgca tcgggggaaa agttgagcga cgagtcgcta caggcactcg
     3481 gcgagctgcg ccgccgcgcc ctgccttacg acgtgcagat cgtcgtctcg gacgggctga
     3541 acgcgctgtc gatcatggac gaggggcacc tcgcgccgtt tctgacgcgg ctgcgggccg
     3601 cgctaaaaga cggcggcctg cggccagcgc cgcagaacct ggtgtttact tccggccgcg
     3661 tgcgggccgg ctaccgcgtc ggcgaggcgc tgttcggcgg catgagcggc gtgcggagca
     3721 tcgtgcacgt catcggcgag cgccccggca cgggacatca cacgttttcc gcctacatca
     3781 cggccgctcc aggaaccgcg tgggcgacct cgggcaagat cgaccacgac atcaccaagg
     3841 tcgtttccgg catcgcctcg acggcgcagc tgccggcaca agccgcagac gaaacggccc
     3901 gcttgctgct ggggttgttc gcccgcatgt cgcagcacaa ataagcgccc tcgactttat
     3961 ttggcgcgcc tcttgcacca cgcgagtcca cgcccgctaa tacgtcttgc gggtcgggag
     4021 gtcacctatg gtgctgttcc ctcttcgccg ccggtcctct gccaccggca atcctcgcac
     4081 cgtcgtccag tgctattcgc tgttcgagca cttcttccct ccctgcggct tgctcgacta
     4141 caccgaaggc atctatcacg gcgatgcgtc gctgccaatc gaggtcgccc agcagaatca
     4201 gctcgagtac gtgctcgacg aggccggctg caccgccggc tcgcggatcc tcgagatcgg
     4261 ctgcggcaac ggcacgctcc tggaagccgc tacccgccgc ggcgcacaag ccgtggggat
     4321 caccatttcg cccgagcagg tcgcccgctg ccgacggcgg aatctcgacg cccggctgct
     4381 gaattacctc gagctcgacg tcgaatgggc ccgccggttc gacgccgtca tcgccaacgg
     4441 gccgatcgag cacttcgtgc agccgcgaga ggccgccgcg gaccggtccg acgccattta
     4501 ccggcagctg ttcgacatct gccaccggat gatcgatccc cgctcgagcg tccgccggct
     4561 ggtgaccacg acgattcact tcatccgccc gcccgagccc gcgaacctgc tcaagagtcc
     4621 gctggccttc ccgcggggca gcgacaattt ccactgggcc atgctggccc gcagcttcgg
     4681 cggctggtat cccgtacccg gccaattcga gcgctgcgcc gccggccgct tttcggtcgt
     4741 gcgcatgacc gacggcacgc acgactatca cttgacgagc gaagaatggc tgcgccgcat
     4801 ccgggctgtc ttgccgacgc tcaagggctg ccggctctta ctcggcgcgg tgccgttcgg
     4861 cctccgccat ccggtgcagt gcgcgacgat gttcgcctgc atgctcgcct cggaatcgtg
     4921 gaactggcag ttccgcggcc ccgcgccgcc cacgcagctg ctgcggcaca cctgggcgta
     4981 cagcgcatga gcgctacctg gtaatcgctg ccagccggcc tgggcatctc gatttctcgc
     5041 tgaattcgac tactttgaga gcagctccaa ctcgaccagg attcccatgc ccagtccgct
     5101 tctcgacagt acgaaccgca tcctgcccgc cctcgtcacg ccgctcttgt ccgatgggcg
     5161 gctcgacgag aagagcgccg agcggctgat cgatcatctc tatgccacag gcgtcggcgg
     5221 gctgtatctc accggaacca ccggcgaagg catctatctc gatttcgaca tccggcggcg
     5281 gatcgtcgag atcgcctgcc gccagtcgca gggccgcggc caggtgatcg tgcacgtggg
     5341 cgcgatccaa gcggccaagt ctctagagct gggggcgcat gccgcgaaag ccggcgcgga
     5401 cgcggtcagc agcatcccgc cgtttgccgg cggctattcc ttcgacgagg tgcgggcgtt
     5461 ctatgccgag ctcgcgaagc agagcccgct gccggtcgtc gcctactaca ttccggctct
     5521 caccggccag cagcattcga ttgccaacct cgccgcgctc ttgcggctgc cgaacgtcgc
     5581 gggcctcaag ttcaccgacc cgaactttta cacgatgcag cggctgctga cgcggctgag
     5641 cgccgagcag gtgctctaca acgggcagga cgaggccctg gccctcggcc tgcaagtcgg
     5701 cgcgcacggc ggcatcggca cgacttacaa cttcatgccc gagctgatcc tggagatcta
     5761 ccagcattgc caggccggac gctatgccga ggccctcgcc gcgcagcgaa aggccaacga
     5821 tatcatcgaa gccctgctaa tgttccacgt ggtcgccgca gcaaagcaga ttctctactg
     5881 gcaaggcctg atcgaccatc cgcactgcgc cttcccgcgg gccttgctca cgtccgccca
     5941 gcaaggtcaa ctgcgcgacg cgctctcaac gacctccatc gctcacacac tcgttcgcta
     6001 aggtgccagc catgatttcg caggcattca attctcacac agaggcacag aggcacagag
     6061 atttcggacg gaccgcaacg agctggtccg ctgtttgtct gctccgtgcc tctgtgcctc
     6121 tgtgtgcgat tctggctctt ggcgtcattc tggtgacggt ctgcaccacg acagcaaacg
     6181 gccaatccct caaggccggc gcggccacga gcaacatcac gccgccgatc ggggcacaga
     6241 tcgtcggcgg ccacctgccg gttccttcga cgcacgtcca tgacgaactg cacgcccgct
     6301 gcctggtgct cgacgacggc cgcaccaagc tggcgctggt cacgtgcgac gtggtcggcg
     6361 tccaccggct cgtctgcgag gcggcgaagg agctgatcca ggcggaagcc ggcattccgc
     6421 ccgcgaacgt tctgatctcg gcgactcaca cgcactcggc ggccagcgtg cagggccagg
     6481 accgcttcaa gcattcggaa tcgctcgacg agtatcaaaa gttcgtcgtc cgccggatcg
     6541 ccgatggcgt gcggcgggcc gcgactaacc tgcgtcccgc ggaactcggc tacggtacgg
     6601 ctcaggctcc cgagcacctc ttcaatcgcc gctggtttct caaacccgga acgatgcccg
     6661 aaaacccctt cggcggcatc gaccaggtga agatgaaccc gggcaccagc ccgaacctcg
     6721 tcgagccggc cgggccgacc gatccgacaa tctcgttctt tgcggtccgc gagccgggcg
     6781 gccggccgat ttcgctctat gccgcgtatg ggctgcacta cgtgggcggc accggcggtg
     6841 gcgcgatctc cgccgattac ttcgccgtct tctgcgacga aatccagcgg ctgctcgggg
     6901 ctgatcggct cgatccgcca ttcgtggcac tgctggccaa cggcaccagc ggcgacgtga
     6961 acaacatcaa cttccgcgag ccgcggccgc cccagaagcc ctacgagcag attcgctacg
     7021 tcgccggcga cgtcgcggcg aaagtccacg cggcaatgga aaaggtccaa taccagaccg
     7081 acgtccgcct cgacgcccgc ctgcgcgagc caacgctccg ctggcggcgg ccaagtgccg
     7141 acgagctcgc ggccgcgaag aagcgatttg ccgaaggcgt gaaagtcgcc cgcggggcgg
     7201 acctgccgta cctctatgcc gagcgcgcaa tcaagctggc cgagtatccc gagacgacga
     7261 cggtgccgat tcaggtgctg gcgatcggcg acctctcgat cggcacgatg ccctgcgagg
     7321 tctttaccga gacgggtctg gagtggcggc aagtctcgcc gatccagccc gcgttcttcg
     7381 tcgagctggc caacggctcc ttcggctacc tccccacgcc gcggcagcac aagctcggcg
     7441 gctacgaaac ctggctcggc accaaccggc tggagatcga ggcctccgaa aagctgctgg
     7501 ccgagctcgt cgcaatggcc ggcgagatca aagccgcgcg gcaggccaaa tgaaacacga
     7561 agaacactaa ggacacgaag gaacctattt gaaatctcct tcgtgatctt cgtgaccttg
     7621 gtgtttaatt cccatccgct ggatcgcagg gagagttgcc aatgcgttgt tttcctttgc
     7681 tcatcctctt cggtgcttcc gttgtcgctg cgcaaccgcc gcaagcgcga atcgcggctt
     7741 cgcacggata cgacaaggcc gtggagctga tgcgcggcaa tgctcgggca gttctgtgcc
     7801 ccgagatcgg cgggcgcgtg ctggaattct ccgtcggcgg caaacaggcg atgtggttcg
     7861 acgaagcgga acgcacgcgg cagccgggcg gcccagcgcc ctccagcgcc ggccggttcg
     7921 attttggccc ggagatgacg acgccccggc atatgctcat ctggggcggc gcttggaccg
     7981 cggagattac cgggccgtct tccgcgcgcc tgacgagcca gaaggatgcc tccagcggcg
     8041 tgcagctgat gcgcgacttc gaactggccg aatccaaagc cgcgaccagt ggtcgcggtc
     8101 cccagatacg cctcatctgc aagcagacga tcacgaaca
//
LOCUS       DASRJB010000004         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1976156, whole genome shotgun sequence.
ACCESSION   DASRJB010000004 DASRJB010000000
VERSION     DASRJB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976156"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFB80_00095"
     CDS             complement(<1..277)
                     /locus_tag="VFB80_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32174.1"
                     /translation="MDLPGLAQPGVRTRRLARGPQSAAIDAPLVVGQWRLTRLLHRGE
                     RLALYRAAPAGEKIGPGCYAIKTPLADRRLDELALALLAREALVAAQV"
     gene            521..1690
                     /locus_tag="VFB80_00100"
     CDS             521..1690
                     /locus_tag="VFB80_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN32175.1"
                     /translation="MLASFGRSFQSLAVLLSLSMLAGCGTQGGGGSGATTTTGGRPGA
                     SEGSGSAGAGTKRIMFVTNGDDPFWDACLSGLNEGAKQHELEKAGLSVHRDVNNGTAE
                     GQIEKLRQYATQDDIAGVAISVIQADNSAIVEEMRNLQEKGVHVITVDGDVNRKLYRN
                     ARTYYVGTDNIVGGRALGTAAKALLEAKEVKSGGYVQFAGFTDNDNARSRMDGVQAMI
                     GTAYEEFSREADDMNLQRARDNVRNAILNRGDKLVALIGIWAYNAPAIADVVSETKTR
                     DKLVVATFDAQAGAIVDMEQGRIDVMVVQNPFDMGVQTCRIMKAMIAHDEATLKQLFP
                     NQGQPDGDIYTTGLRVVVPSDKSPVKPGLFDPKVVEYMLLPDFRAWLNKYKLQSS"
     gene            1687..2934
                     /locus_tag="VFB80_00105"
     CDS             1687..2934
                     /locus_tag="VFB80_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN32176.1"
                     /translation="MSSSAARLGRPALPLNELALVLSIVLVFVLTGLIDRNHTYFTNF
                     QASFVIILRNTVLLGIVALGAAVVIIAGGIDLSAGSMIAFSATTCALIMVLLAAPSDR
                     MLLEVGPWGAICGIAGGLIAGLLVGTLHTWLITSIRLPPFVATLATLVGLRSFARAMC
                     EFITRQRWGSQYQQVNVNAPLFIYIKEHLWIATTVFLVLALVTWIILSRTVLGRHIYA
                     LGGNEQAARLSGIRTDSVKWFAYCFGALTASIAGIFAMADGSVAQPVNLARGFELNAI
                     AAAVVGGCSLQGGVGTVLGTILGALFLRLVIDAVAKIIKAGADVYEGMIVGIVVVLAV
                     TLTQVRQLLQSGREFFPGLHGALVIPTLAISAGLLAMMGGANVEVLKGRTVWFGVAVG
                     VVTFLLLTAVKLLEMQRRPKTSN"
     gene            2934..>3826
                     /locus_tag="VFB80_00110"
     CDS             2934..>3826
                     /locus_tag="VFB80_00110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZN32177.1"
                     /translation="METVPIISIQRVTKRFPGVVALEDVTLDVRAGELHAICGENGAG
                     KSTLMKILSGVLTEYEGELLLEGKKVAFQGTRDAEAAGVSIIHQELNLVEQLSAAANI
                     FLGREIRGWLGWRDDRAMELAAARLLKELDCEIDPRALAGSLRVGDQQLIEIAKALSL
                     QSDILIMDEPTSALTEAEVGRLYRVIARLRQRGVTILYISHKMDEVFHLADRITILRD
                     GRLVKTLDTKATSPREVTHLMVGREIEAIHFGGRRQAGNVLLKVTNLSLPWPGHARGW
                     RLKDVSFELRRGEVLGIAGLMG"
BASE COUNT          673 a         1200 c         1285 g          668 t
ORIGIN      
        1 caacctgggc cgccacgagc gcttcgcgcg cgagcagcgc cagggccagt tcgtccagcc
       61 ggcggtcggc cagcggcgtt ttgatcgcgt agcagccggg gccgattttc tcgcccgctg
      121 gcgccgcgcg atacagtgcc agccgctcgc cgcggtgcaa gaggcgcgtc aatcgccact
      181 ggccgaccac cagcggagcg tcaatcgccg cggattgcgg tccgcgggca aggcggcgcg
      241 tgcggacgcc gggttgggcc aggccgggca aatccatggc gggccggaga aaagcggtac
      301 ggcatcgctg gtgggtagcc agcagccgcg gcgggcggag gaggggctat cgcttccatc
      361 ggcgggagca agcgggtgca accattgcag cagctcctgc cgtcacagat taccagcggc
      421 ttgcggcagc gccaggacag aactagaatc ccaaactccc ttgggcggcg gtctcacgcc
      481 gccgctttgt agaattcaga tgcccgtcgg agccctctcc atgctcgcct cgtttggccg
      541 atcgtttcag tccctggcgg ttctgctctc cctctcgatg ctggccggct gcggcactca
      601 aggcggcggt ggcagcggtg ctacgacgac tacgggcggc aggccaggtg ccagcgaagg
      661 cagcggatcg gcgggtgcgg ggacgaaacg cattatgttc gtcaccaacg gcgacgaccc
      721 gttttgggac gcctgcctca gcgggctgaa cgaaggcgcc aagcagcacg aattggagaa
      781 ggcgggtctg tcggtgcatc gcgacgtcaa caatggcacc gccgaaggtc agatcgaaaa
      841 gttgcggcag tacgccacgc aggacgatat cgcgggcgtc gcgatctccg tgattcaggc
      901 cgacaactcg gcgatcgtcg aggagatgcg gaacctgcag gagaagggcg tgcacgtcat
      961 cacggtcgat ggcgacgtga accgcaagct gtatcgcaac gcccgcacgt attacgtcgg
     1021 cacggataac atcgtcggcg gccgcgcgct gggtacggcg gccaaggccc tgctcgaggc
     1081 gaaagaagtc aagagcggcg gctatgtcca gttcgccggc ttcactgaca acgacaacgc
     1141 ccgcagccgc atggacggcg tccaagcgat gatcggcacg gcgtatgaag agttcagccg
     1201 cgaagccgac gacatgaatc tgcagcgggc tcgcgacaac gtccgcaatg cgattctgaa
     1261 tcgcggcgac aagctcgtag cgctgatcgg catctgggcc tataacgcgc cggccattgc
     1321 cgacgtcgtg tcggaaacga aaactcgcga caagctcgtc gttgcgacct tcgacgccca
     1381 ggccggcgcg attgtcgaca tggagcaggg ccgaatcgac gtgatggtcg tgcagaaccc
     1441 gttcgatatg ggcgtgcaaa cgtgccggat catgaaagcg atgatcgccc acgacgaggc
     1501 cacgctcaag caactgtttc cgaatcaggg ccagccggac ggcgacattt acaccaccgg
     1561 cctgcgggtg gtcgtgcctt ccgacaagtc gcccgtcaag ccggggctat tcgacccgaa
     1621 agtcgtcgag tacatgctgc tgcccgattt tcgcgcctgg ctgaataagt acaagctgca
     1681 aagttcatga gctcttccgc tgcgcggctg gggcggccgg ccctgccgct gaacgaactg
     1741 gcgctcgtgc tgtcgatcgt cctggtgttc gtattgaccg gtttgatcga ccgcaaccac
     1801 acctatttca cgaacttcca ggccagcttc gtcatcatcc tgcgcaacac ggtgctgctg
     1861 ggcatcgtgg cgctcggcgc ggccgtggta attatcgccg gcggcatcga cctctcggcc
     1921 ggttcgatga ttgccttcag cgcgacgacc tgcgcgctga tcatggtgct gctggctgcg
     1981 ccgagcgacc ggatgctgct ggaagtcggc ccgtggggcg cgatctgcgg catcgccggc
     2041 ggcctcatcg cggggttgct cgtcggcacg ctgcatacct ggctcatcac ttccatccgg
     2101 ctgccgccgt ttgtggcgac gctcgcgacg ctcgtcggtc tgcggagctt cgcccgggcg
     2161 atgtgcgaat tcatcacacg gcagcggtgg ggcagccagt atcagcaagt caacgtcaat
     2221 gccccgctct tcatctacat caaggagcac ctctggattg ccacgacggt gttcctggtt
     2281 ttggcgctcg tgacgtggat catcctcagc cgtacggtcc tcggccggca tatttatgcc
     2341 ttaggcggga acgagcaggc ggcccggcta tcgggcattc gcacagacag cgtgaagtgg
     2401 tttgcctatt gcttcggagc cttgaccgcc tcgatcgcgg ggatctttgc gatggccgac
     2461 ggcagcgtcg cgcagcccgt caatctggcc cgcggattcg agctgaatgc gatcgccgcg
     2521 gccgtcgtgg gcggctgcag cctgcagggt ggcgtcggca ccgtgttggg caccatcctg
     2581 ggcgcgctct ttttgcggct ggtcatcgac gccgtggcga agatcatcaa ggcgggggcc
     2641 gacgtttacg agggcatgat cgtgggaatc gtggtggtgc tggcggtgac gctgacacag
     2701 gtccgccagc tcctgcaatc gggccgcgag ttctttccgg gcctgcacgg agcgctggtg
     2761 attcccacgc tggcgatctc ggcggggctc ctggcgatga tgggcggcgc caacgtcgag
     2821 gtgctcaagg gacgcacggt gtggtttggc gtcgccgtcg gcgtggtgac attcctcttg
     2881 ctgactgccg tgaaactctt ggaaatgcag agacgtccaa agacgtcgaa ctgatggaaa
     2941 cggtcccaat tatctcgatc cagcgcgtca cgaagcgttt tcccggcgta gtggccttgg
     3001 aagacgtaac gctggacgtc cgcgcgggcg aactgcatgc aatctgcggc gagaatggag
     3061 cgggcaagag cacgctgatg aaaatcctgt cgggcgtgct gaccgagtac gagggggagc
     3121 tgctgctgga gggaaagaag gtcgcgttcc agggcacgcg cgacgccgag gcggccggcg
     3181 tgagcatcat tcaccaggag ctgaacctcg tcgagcagct ctcggccgcg gcgaatatct
     3241 tcctgggccg cgaaatccgc ggctggctcg gctggcgcga cgaccgggcc atggaactgg
     3301 ccgccgcgcg gctcttgaaa gagctggact gcgaaatcga cccgcgggcc ctcgccggca
     3361 gcctccgcgt cggcgatcag cagctcatcg agatcgccaa ggccctctca ctgcaatccg
     3421 acatcctgat catggacgag ccaacgagcg cgctcaccga agccgaagtc ggccggctgt
     3481 accgcgtcat cgcacggctc cggcagcgcg gcgtcacgat cctctacatc tcgcacaaga
     3541 tggacgaggt ctttcacctc gccgaccgca tcacgattct ccgcgacggc cggctggtga
     3601 agacgctcga caccaaggcc accagtccgc gggaagtgac gcacctcatg gtcggccgcg
     3661 agattgaggc catccatttt ggcggccggc ggcaggcggg caacgtgctc ctcaaggtca
     3721 ccaatctctc gcttccctgg cccggccacg cccgcggctg gcggctgaaa gacgtgtcgt
     3781 ttgagctgcg ccgcggcgaa gtgctgggca tcgccggcct gatggg
//
LOCUS       DASRJB010000005         5934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2540905, whole genome shotgun sequence.
ACCESSION   DASRJB010000005 DASRJB010000000
VERSION     DASRJB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540905"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFB80_00115"
     CDS             complement(<1..1352)
                     /locus_tag="VFB80_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32178.1"
                     /translation="MRRLLLASLVVFSLPAVLRAAEAVTFNKDIAPLVFRHCAGCHHP
                     GEVAPFSLLSYADLSKRARQVQEVTSRRYMPPWKGVEGHGRFVGERRLTAEQIELIGK
                     WVEQGAAEGDPKDLPPQPQFTEGWKLGPPDIVLTMPEPYEIPADGPDIYRNFVFDVKI
                     PEGKYLKAAEYRPGNRRIVHHAALAIDLDGKARREDEADPLPGAKGSLSIPGRLLPGS
                     LSAWTPGRDAAPLPEGISFPWQAGAGLMLQLHLHPSGKPESEQSSVGLYLTDQPPKRS
                     MADLALIARKIDIPPGEKEYRSRDEFTLPIEMQVLGIFPHMHLIGKDIKITAHPPEGE
                     PFSLIWISDWDFNWQGFYECAAPVKLPAGTKVVLETIHDNSAENFRNPFQPPQRITYG
                     EQTSNEMSAAILQLVPDQDAELPKLRETLRGRVIGTIRAATAESADEPVFARIAKDLL
                     A"
     gene            complement(1356..1865)
                     /locus_tag="VFB80_00120"
     CDS             complement(1356..1865)
                     /locus_tag="VFB80_00120"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN32179.1"
                     /translation="MPPLIDLNGKPLTPFEKPGTKAVALIFVLPDCPIANAYLPAINR
                     LHAEFASQGVALIVVQADPATTAERARAHAKEYGIAAPVILDAEHAWVRRARATRTPE
                     AVVFSPAGEILYRGRIDDQYVGLGQKRPQATTYDLQDALAAIVRGQNVSQRETPAVGC
                     YIPPLPKGK"
     gene            complement(2114..4036)
                     /locus_tag="VFB80_00125"
     CDS             complement(2114..4036)
                     /locus_tag="VFB80_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN32180.1"
                     /translation="MVVTSRAGRWSLTIVAVLLCANALAGVAAGQTLSDDVYRGLREF
                     YRRTARTDGSFQPGIDPSYQGMSDSAFSDMAAVTYACVIHKTFGWELPHREKTVEWLL
                     ARQQPGGEFVNVTGTVDPRSPQGRTYNTTQALVALRALGERPHFDPLPVFEEIMRQDY
                     KTLPAYSTSFFPLAYLCAGKPIPAQADKAIRALMVQAGDGYLNDHVAATFHASHYYSL
                     VGEPTPKAELMVARMLRDQNPNGGWLLNTPSRDRHATFDAVFTLRHEGGGQPDCQAAI
                     SRAAKWALSCRNADGGFGHFPGSTSDADAVYFQVGTLVMAGVLAPADPLPSDPHLLGW
                     GHLMPLRKPAASDPVRVTLPAWVGGLGFSPDGKQLAIACADGVTQLTDSRSGRLLAAL
                     KGHSDCVAAAAFSPDGRRLTTGSFDHTANIRDLQTGMVAHPLVGHAGAVLSVAYAPGG
                     SHVATASIDQTIRLWDAFTGQHERTLRGHKSWVNSIAFMPDNERLISGSSDGTVRVWS
                     VASGELLKTIEATPAEVRSIAISPDGKLVAAGIRYGVIKLWNTSDWSERRSWTSAGDD
                     VWAVAFSTDSKRLFSAAGEWNRPAAVVAWDVDTGEEIARHQHTGEVLCLAVSPDGRQL
                     AAAGGDRTVSVWTLRP"
     gene            4174..5667
                     /locus_tag="VFB80_00130"
     CDS             4174..5667
                     /locus_tag="VFB80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HZN32181.1"
                     /translation="MAFRFARAACFAAALSVLASPALADIRLPALFTDHMVLQQAQKN
                     RVWGWADPGEEVIATIAGQRHTAKADDKGKWLLTLDPLAVGGPHTLSLAGKNKLAVED
                     VLVGEVWICSGQSNMQWSVSQSNDADLEIKTAKYPRIRFISVPQVGTQEPQDNFQGSW
                     KVCAPNTVGELSAVGYYFGRQLHQTLDVPIGLIDDAWGGSACEAWIRRDILAQDEQYK
                     PLLDRWAQIEATLADAVKSKAKGAPNPNVLTGNARPGNIYNGVLKPTIGYGIRGAIWY
                     QGETNAGRAYQYRELFPLMIKSWREEWQQGDFPFYWVQLADFMAEKDQPLDSSWAELR
                     EAQTLTMGKLPNTGEAVIIDLGEGRDIHPRNKQGVGLRLARWALARDYGLSDLAHQSP
                     RYKSMEKQGSKIVLSFDHVGAGLYTFDVTKPIGFAIAGSDQKFVWADAVLEGKDKIVV
                     ISPQVAEPAAVRYAWADNPVCNVYSREGLPLTPFRTDDWKGVTADAK"
     gene            5722..>5934
                     /locus_tag="VFB80_00135"
     CDS             5722..>5934
                     /locus_tag="VFB80_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32182.1"
                     /translation="MTTNLQLKQIPVAKTGMLIRRPVADVFEAFVNPDVTTQFWFTNG
                     SRRHEAGNQVQWDWEMYGISIPVNVKS"
BASE COUNT         1051 a         1937 c         1954 g          992 t
ORIGIN      
        1 gccagcaggt ccttggcaat acgagcgaaa accggctcat cggccgattc ggcggtggcg
       61 gcccggatcg taccgatcac gcggccccgc agcgtttcgc gcagcttggg cagctcggcg
      121 tcctggtccg gcacgagctg caagatagcg gccgacatct cgttgctggt ttgctcgccg
      181 taggtgattc gctgcggcgg ctgaaacggg ttgcggaagt tttcggccga attgtcgtgg
      241 atcgtctcca ggacgacctt cgttcccgcc ggcagcttca ccggcgcggc gcattcatag
      301 aagccctgcc agttgaaatc ccagtcgctg atccaaatca gcgagaacgg ctcgccctcg
      361 ggcggatggg ccgtgatctt gatgtccttg ccgatcaggt gcatgtgcgg aaagatgccc
      421 agcacctgca tctcgatcgg cagcgtgaac tcgtcgcgcg agcgatattc cttttcgccg
      481 ggtggaatgt cgatcttgcg ggcgatcagg gcgaggtccg ccatcgaccg cttgggcggt
      541 tgatcggtca ggtacaggcc gaccgacgac tgctcgcttt cgggcttgcc actaggatgc
      601 aggtgcaact gcagcatcag ccccgcgccc gcctgccagg gaaacgagat gccctcgggc
      661 agcggcgcgg cgtcgcggcc cggcgtccag gctgagaggc tgccaggcaa caggcggcct
      721 gggatcgaca ggcttccctt ggcccccggc agcgggtcgg cctcgtcctc gcggcgggcc
      781 ttgccgtcca ggtcaatcgc caacgccgcg tggtgcacga tgcggcggtt gcccggccga
      841 tactccgcgg ccttgaggta tttgccttcc gggatcttca cgtcgaagac gaaattgcga
      901 taaatgtccg ggccgtcggc cgggatttca tagggctcgg gcatcgtcag cacgatgtcg
      961 ggcggcccga gcttccagcc ttcggtgaat tgcggctgcg gcggcaggtc tttggggtct
     1021 ccttcggctg cgccttgctc gacccacttg ccgatcagct cgatttgctc ggccgtgagc
     1081 cgccgctcgc cgacgaaccg gccgtgcccc tccacgcctt tccagggcgg catgtagcgc
     1141 cggctggtca cctcttgaac ctgccgggct cgcttgctga ggtcggcata gctgaggagc
     1201 gaaaacggcg cgacttcgcc cggatggtgg cagccggcgc aatgccggaa caccaagggc
     1261 gcgatgtcct tgttgaaggt cacggcctcg gcggcgcgga gcactgctgg cagagaaaac
     1321 acaactagcg aagccagcag gaggcggcgc atgcgtcact ttcctttcgg caaaggcgga
     1381 atgtagcagc cgacggccgg cgtttcgcgc tgagaaacgt tctgaccgcg gacgattgca
     1441 gccagcgcgt cctgcaggtc gtacgtggtc gcctgcggcc gcttctggcc caatccaaca
     1501 tactggtcgt cgatccgccc gcgatagagg atttcgcccg ccggcgaaaa gacgaccgcc
     1561 tctggcgtgc gcgttgctcg cgcccgccta acccaggcgt gctccgcgtc gagaatgacg
     1621 ggcgctgcga tgccatattc cttggcgtga gctcgcgccc gctccgccgt cgttgcggga
     1681 tcggcctgca cgacgatcag cgccacgccc tgcgaagcaa actccgcgtg caaccggttg
     1741 atcgccggta gatacgcgtt ggcgatggga caatccggca ggacaaagat cagcgcgacg
     1801 gccttcgtgc cgggtttctc gaagggcgtc agcggcttgc cgttcagatc aataagtggg
     1861 ggcaatgccg caggtgcgga cgggtcggct tgttttgttt gggtgcagcc aagcaacagt
     1921 attgacgaca ggacgaaggc aatatgcagg agagcctcag gcccggaggg ccgacaggca
     1981 gtagccccga ccgtcaggtc ggggattggc gacggcccaa gaatcaagca aggcccggag
     2041 ggccgacacc gacgcaatgt gagtttctcc atttcctgtg ccggccctcc gggcctgcgg
     2101 tgctgctatc accctatggc cttagagtcc acacgctgac cgtgcggtcg ccgccggcgg
     2161 cagccagttg cctgccgtcg ggcgagaccg ccaggcacaa gacttcgccc gtgtgctgat
     2221 ggcgggcaat ctcttcgccg gtatcgacat cccaggcaac gacagcagct ggccggttcc
     2281 attcgcccgc ggcgctaaac agccgtttgc tgtcggtcga aaacgcaact gcccagacgt
     2341 cgtcgccggc gctggtccag gagcggcgct cgctccaatc ggacgtattc cacaacttaa
     2401 tgacgccgta gcgaattccc gcggcgacca gcttgccgtc agggctgatc gcgatcgagc
     2461 ggacttcggc cggcgtcgct tcgatcgtct tcaagagctc gccgctggcg accgaccaga
     2521 cccgcaccgt cccgtcggaa ctgccggaga tcagccgctc gttgtcgggc ataaaggcga
     2581 ttgaattcac ccaggacttg tgtccgcgaa gcgtccgctc gtgctggccc gtgaaggcgt
     2641 cccagagacg gatcgtctgg tcgatgctgg ccgtagcgac gtgagagccg cccggcgcgt
     2701 aagccacgct caagaccgcg ccggcgtggc ccaccagcgg atgcgccacc atgcccgtct
     2761 gcagatcgcg gatgttggcc gtgtggtcga agctacccgt ggtcagccgc cggccgtcgg
     2821 gcgaaaaggc cgcggccgcg acgcagtcgc tatgcccctt cagcgcggcg agcagccggc
     2881 ccgagcggct gtcggtgagc tgcgtgacgc catcggcgca ggcgatcgcc agttgcttgc
     2941 cgtcggggct gaagcccagg ccaccgaccc aagcaggcag cgtcacgcga acgggatcgg
     3001 acgcggccgg cttgcgcagc ggcatcaggt ggccccagcc gagcaggtgc ggatcgctgg
     3061 gcagcggatc ggccggcgcg agcacgccgg ccatcaccag tgttccgacc tggaagtaga
     3121 ccgcgtccgc atccgaggtg gagccgggaa agtgaccaaa gccgccgtcg gcattgcggc
     3181 aggacagcgc ccacttcgcc gcgcgggaga tcgccgcctg gcaatcgggc tggccgccgc
     3241 cttcgtgccg tagcgtgaag accgcgtcga acgtcgcgtg ccggtcccgg gacggcgtgt
     3301 tgagcagcca gccgccgttg ggattctggt cgcgcagcat gcgggccacc atcagttcgg
     3361 ccttcggcgt cggctcgcca accaggctgt aatagtgcga cgcatgaaac gtcgccgcga
     3421 cgtggtcgtt caagtagccg tcccccgcct gcaccatcag cgcgcggatc gccttgtcgg
     3481 cctgggcggg aatcggcttg ccggcacaga gatacgccag cggaaagaag ctcgtcgaat
     3541 acgccggcag cgtcttatag tcctgccgca tgatctcttc gaacacgggc agcggatcga
     3601 agtgcggccg ctcgcccaga gcccgcagcg cgaccagggc ctgcgtcgtg ttgtaggtgc
     3661 gtccctgcgg cgaacgtgga tcgaccgtcc ccgtcacgtt gacaaattcg ccgcccggct
     3721 gctggcgggc cagcagccac tctaccgtct tttcgcggtg cggcagctcc cagccaaacg
     3781 ttttgtggat cacgcaggca tacgtgaccg cggccatgtc gctaaacgcg ctgtcggaca
     3841 tgccctggta gctcgggtcg atgcccggct gaaacgagcc gtcggtgcgg gcggttcgcc
     3901 gatagaactc gcgaagacct ctgtagacgt cgtcgctcag agtttgcccg gcggccacgc
     3961 cggccagcgc gttcgcacaa agcaagaccg ccacgatcgt gagcgaccaa cggccagcgc
     4021 gagatgtcac caccatagaa gcccccacca gcaaaggtgt cgaatcattt cgataatagc
     4081 tcaactgacc ggccgctgcc gcctgtttac aagcctccgg cggtagcgca cagtagcagt
     4141 gcctaaaggt ccaacgaacc ggagagattt gccatggcgt tccgttttgc tcgcgcggca
     4201 tgcttcgcgg ccgccctgtc cgtgctcgcg tcgccggctt tggccgacat tcgcctgccg
     4261 gccctgttca ccgaccacat ggtcctgcag caggcccaga aaaatcgcgt ctggggctgg
     4321 gccgatccgg gcgaggaagt gatcgctacg atcgccggcc agcggcacac ggccaaggcc
     4381 gacgacaagg gcaagtggct gctcacgctc gacccgttgg cggtcggcgg cccgcacacg
     4441 ctcagcctgg cgggcaagaa caagctcgcc gtcgaggacg tgctggtcgg cgaagtctgg
     4501 atttgttccg gtcagtcgaa catgcagtgg tcggtctcgc agtcgaacga cgccgacttg
     4561 gaaatcaaga ccgccaagta tccgcgcatc cgcttcatca gcgtgcccca ggtcggcacg
     4621 caggagccgc aggacaactt tcaaggcagc tggaaggtct gcgctcccaa cacggtcggc
     4681 gaactctcgg ccgtcggcta ctacttcggc cggcaactgc accagacgct cgacgtgccg
     4741 atcgggctga tcgacgacgc ctggggcggc tcggcctgcg aggcctggat tcgccgggat
     4801 atcctggctc aggacgagca gtacaaacca ctgctcgatc gctgggcgca gatcgaagcc
     4861 acgctggcag acgcggtgaa gtcgaaggcc aagggtgcgc cgaacccgaa cgtgctcacc
     4921 ggcaacgccc gccccggcaa catctacaac ggcgtgctca agccgacgat cggctacggc
     4981 atccgcggcg cgatctggta ccagggtgag acaaacgccg gccgcgccta tcaatatcgc
     5041 gagctatttc cgctgatgat caagagctgg cgcgaggaat ggcagcaggg ggacttcccg
     5101 ttctactggg tgcaactcgc cgatttcatg gccgagaagg accagccgtt ggacagctcg
     5161 tgggccgagc tccgtgaggc ccagactctg acgatgggca agctgccgaa tacaggcgaa
     5221 gcggtgatca tcgacctcgg cgaaggccgc gacattcatc cccgcaacaa gcagggcgtc
     5281 ggcctgcggc tggcccgctg ggcgctggcc cgcgactacg gcctgtcgga cttggcccat
     5341 cagagcccgc gctacaagag catggagaag caaggcagca agatcgtgct gtcgttcgat
     5401 cacgtcggcg cggggctcta cacgttcgac gtcacgaagc cgatcggctt cgccatcgca
     5461 ggcagcgacc agaagttcgt ctgggccgac gccgtgctgg agggcaagga caaaatcgtc
     5521 gtaatcagcc cgcaggtcgc cgagccggca gccgtccgct atgcctgggc cgacaatccg
     5581 gtctgcaacg tctacagccg cgagggcctg ccgctgacgc cgttccgcac tgacgattgg
     5641 aagggcgtca cggccgatgc gaagtagccg agctccctcc ctgtgggaga gagctagggt
     5701 gagggtggtt aaggaggcaa catgaccacc aacctccagc tcaagcagat ccccgtcgcc
     5761 aagaccggca tgctcattcg ccggccggtc gccgatgtat tcgaggcgtt cgtcaacccc
     5821 gacgtcacca cgcagttctg gttcaccaat ggaagccgtc ggcatgaggc cggcaaccaa
     5881 gtgcagtggg attgggagat gtacggcatc tccatcccag tcaacgtgaa gtcg
//
LOCUS       DASRJB010000006         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3951732, whole genome shotgun sequence.
ACCESSION   DASRJB010000006 DASRJB010000000
VERSION     DASRJB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951732"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFB80_00140"
     CDS             <1..184
                     /locus_tag="VFB80_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HZN32183.1"
                     /translation="NGQPFNKNDWANRHGFKSRHPTGVQFALCDGSVRFINDSIPLGL
                     YRAMATIDTGEVVSAP"
     gene            338..1723
                     /locus_tag="VFB80_00145"
     CDS             338..1723
                     /locus_tag="VFB80_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32184.1"
                     /translation="MHASPFTVSRRKFLAAGVIGGGLTLANLLRAEAAAGVGSSHKAV
                     INVHLDGGPPQMDLIDLKPQAPAEIRGEFTPIPTAIPGVQICEFLPKLAAAADKLVFI
                     RSLVGAASAHDAFQCMSGFDAKNLASIGGRPAMGSIVAKLRGTAADVSPSFVDLMQGR
                     PLVRNSARPGFLGPTYQAFRPDMSQLFERPLEPGMVTELANKGQNHTIALSLSDGLSL
                     DRVHDRTSLRQSLDRLKGEIDKSGMMHALDAFSQQAVGILTSGRFAAALDLSKEDPRV
                     IQRYALANASAEPASTTSDRPNATLKFLLARRLVEAGARCVSISISDFDTHSNNFPRM
                     RKVLPIVDHGLATLLADLEERGMLGDVSIVAWGEFGRTPKVSGNGGRDHWPAVGMALL
                     AGGGIRAGQVIGETDRYAAAAISRPVHYQDVMATLYQNLGINARATTITDPSGRPQYL
                     LDRGEALRELV"
     gene            1942..>4235
                     /locus_tag="VFB80_00150"
     CDS             1942..>4235
                     /locus_tag="VFB80_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32185.1"
                     /translation="MSRLPREFSWHLFAFIVVVAGLGALAPLAWWQTRAPGDSAAQPQ
                     LAPLRPLVADKIDLDPSVTETPALPAANSPAMNQELLADATGDLRLSANNSRLPALPQ
                     IPGALDTQPLREPAETLPQPLPRSLPPTKAWPLPAGLIEQINALEATVPPAADWTRRV
                     KVEIGRLAAVDSLADPAAEAPLAGLKRLAEEGKTLAAELSRDEERSKLLRAGYAIVRR
                     LVVWDQVHTLAARAGDGSAPVVDRESWLQALTTVDELLRSTGAASHWRKYLLIDRALT
                     TFDSPECTPADQRELAREILHRMHSTQLSREQENFLKTPPLAALGEQLTARAAERPDL
                     TGLLAAVEQYEHDDKSSNSRQLAAHYDALRWSTEPEVKELADTVNSYYRNANVRVALS
                     ADLLNKMIPQQQPLWEAIEDSILGAWVSGQSQTNTKVKVVLVPDPNRWNIGLEALGEV
                     NTDTSSSKGPATFFQFGEAMFRARKRLTVDRRGIRLFGAEAEANANNYMGDFETDFDG
                     LPLLSPIARAIARNQYEQAQPAAKVEVEGKIIVRATSQLDREVGKRLEKAKRDFQARL
                     VDPLRKLNLEPTAVDLETTPERLIARYRLAARDHVAAHTPRPQAPGDSMLSVQLHETA
                     MNNVLQELNLEGRRIELRELFNEMTTRFSQQQIEIPEDLPEDVYVTFAQQDAVRVDCH
                     DGRVRLTIRIEELEQEGTRNRWTNFTVRGYYGPNADQLDANLVREGIIELIGDKRQLT
                     IGQQLALRAIFNKVLSKNRKLSII"
BASE COUNT          760 a         1474 c         1351 g          650 t
ORIGIN      
        1 caacggtcag cccttcaaca agaacgactg ggccaaccga cacggcttca agagccgcca
       61 cccgaccggc gtgcagttcg cgctctgcga tggctcggtg cggttcatca acgactcgat
      121 cccgctcggc ctctaccggg ccatggccac catcgacacc ggcgaagtcg tctccgcacc
      181 ctaacccgac gcgcgagcga gtgcgagtgg ctgcgactcc ctggctggcg cgttggcttg
      241 gtccgagaag attcatggtt ccctctgcct atccaatcgg ctaaactcac agattgacag
      301 acgccgatgc gatccttgct cgcagaggga gccgaccatg cacgcttcgc cattcaccgt
      361 ctcgcggcgg aagtttctgg cggcgggagt cattggcggt gggctgacgc tcgccaatct
      421 gctgcgggcc gaagcagccg cgggagttgg atcttctcac aaggcggtca tcaacgtcca
      481 tctcgacggc ggcccgccgc agatggacct gatcgacttg aagccgcaag cgccggcgga
      541 aatccgcggc gaattcacgc cgatcccgac ggcgatcccc ggcgtgcaga tttgcgaatt
      601 cctccccaag ctggcggcgg cagccgacaa gctggtcttc atccgctcgc ttgtcggcgc
      661 ggccagtgct cacgacgcct ttcagtgcat gagcggcttc gacgccaaaa acctggccag
      721 catcggcggc cggccggcga tgggcagcat cgttgctaaa ctccgcggca ccgcggccga
      781 cgtctcgccg tcgttcgtcg atctgatgca gggccggccg ctcgtgcgca acagcgcccg
      841 gcctggattc ctagggccca cgtatcaggc gtttcggccc gacatgtcgc agctattcga
      901 gcggccgctg gagccgggaa tggtgaccga actggccaac aagggccaaa accacacgat
      961 cgcgctgtcg ctatcggatg ggctgtcgct cgaccgcgtt cacgatcgca cgagcctgcg
     1021 gcagtcgctc gaccgcctca aaggcgagat cgacaagagc ggcatgatgc acgccctcga
     1081 cgccttttcg cagcaggccg tcggcatttt gacgagcggg cggtttgccg cggcgctcga
     1141 cctgtcgaag gaagacccgc gggtcatcca gcggtacgcg ctcgccaatg ccagcgccga
     1201 gccggcctcg acgacaagcg accggcccaa cgccacgctc aagttcctgc tggcccgccg
     1261 cctggtcgag gcgggcgcgc ggtgcgtcag catctcgatc tccgacttcg acacgcacag
     1321 caataacttc ccgcgaatgc ggaaggtgct gccgatcgtc gatcacggcc tggcgacact
     1381 gctggccgac ctggaagagc gcgggatgct gggcgacgtg tcgatcgtcg cctggggcga
     1441 gttcggccgc acacccaagg tctccggcaa tggcggccgc gaccactggc ccgcggtagg
     1501 catggctctg ctagccggcg gcgggatccg cgccgggcag gtcatcggcg aaaccgaccg
     1561 ctacgccgcc gcggcaatca gccggccggt tcactatcag gacgtcatgg cgaccctcta
     1621 tcagaacctg ggcatcaacg cccgcgccac gacgatcacc gaccccagcg gccggccgca
     1681 gtatctgctt gatcgcggcg aggcgctgcg cgagctggtg taacgtgcgg acggcagaca
     1741 agcggatcaa gattaggact tcttaatcct aatctcttcg ctgctcgccc aaccggcaca
     1801 acttatctga gaatctttgc ggcctgacgg ggttggccag actggcagca actcggtatg
     1861 cgcacgcccc ccgcggggtc gattcttctc gatggtccac cgtaggcggc gcttcgcgca
     1921 cgcttaggcg aggagcatcc gatgagccgg ctgccacgcg agttttcctg gcatctgttc
     1981 gcattcattg tcgtcgtcgc gggtctgggc gcgctggccc cgctcgcctg gtggcagacc
     2041 cgcgcgccgg gcgactcggc cgcgcagccg cagctggcac ccctccgccc gctggtcgcc
     2101 gataagatcg acctcgatcc ctcagtgacc gaaacacccg cgctacccgc cgccaactca
     2161 cccgcgatga accaggagct gttggccgac gccaccggcg acttgcggct aagcgcgaac
     2221 aactcccggc tgccggcgct tccccaaatc cccggcgcgc tcgatacgca gccgctccgc
     2281 gagccggccg agacgctccc gcagcctctg ccccgcagct tgcctccgac gaaggcctgg
     2341 cccctgcccg cggggctgat cgagcagatc aacgcgctgg aagccacggt gccgcccgcc
     2401 gcggactgga cccgccgcgt caaagtcgag attggccggc tggcggctgt cgattctctc
     2461 gccgatcccg cggccgaagc gcctctggcc gggctcaagc ggctggccga ggagggaaaa
     2521 accctggcgg ccgagctgtc ccgggacgag gagcgctcga agctgctgcg ggccggttat
     2581 gcaatcgtcc gccggctggt cgtctgggac caggttcaca cgctggctgc ccgtgcgggc
     2641 gacggctccg cgccggtcgt cgatcgcgaa tcctggctgc aggccctgac caccgtggat
     2701 gagctgctgc gttcgacggg cgccgcctcc cactggcgca agtacctgtt gatcgaccgg
     2761 gcgctgacga cgtttgactc gcccgagtgc acgccggccg atcagcggga gctggcccgc
     2821 gaaatcctgc accgcatgca cagcacgcag ctctcgcgcg agcaggaaaa cttcctgaaa
     2881 acgccgccgc ttgccgcgct gggcgagcag ctgaccgccc gggccgccga acggccggac
     2941 ctgacgggcc tgctcgccgc cgtcgagcag tacgagcacg acgacaagtc gtcgaacagc
     3001 cgccagctgg ccgcgcatta cgacgcactc cgctggtcca ccgaacctga agtgaaagag
     3061 ctggccgaca cggtcaacag ctattaccgc aacgccaacg tccgcgtggc cctctcggcc
     3121 gacctgctca acaagatgat tccgcagcag cagccgctgt gggaggccat tgaggattcg
     3181 atcctgggcg cttgggtttc cggccagagc cagacgaaca cgaaggtcaa ggtcgtgctt
     3241 gtgcccgatc ccaaccgctg gaacatcggc ctggaggctc tgggcgaagt gaataccgac
     3301 acctcgtcga gcaagggccc ggcgacgttc tttcaattcg gcgaagcgat gttccgggcc
     3361 cgcaagcggc tgaccgtcga tcgccggggg atccggctgt tcggcgccga ggccgaggcc
     3421 aacgccaaca actacatggg ggacttcgaa accgatttcg acggcctgcc gctgctcagc
     3481 cccatcgccc gtgcgatcgc ccgcaatcaa tacgaacagg cccagccggc ggccaaggtc
     3541 gaagtcgagg gcaagattat cgtgcgggcc acgagccaac tcgaccgcga ggtggggaag
     3601 cggctggaga aggccaagcg cgacttccag gcccgcctgg tcgatccgct gcggaagctc
     3661 aacctggagc caacggccgt ggacctggag acgacgcccg agcgcttgat cgcccgctac
     3721 cggctggcgg cccgcgacca cgtggcggcc cacacgccgc ggccgcaagc tcccggcgac
     3781 agcatgctca gcgtgcaatt gcacgagacg gccatgaaca acgtgctcca ggagctgaac
     3841 ctcgagggtc gccgcatcga gctgcgcgag ctgttcaatg aaatgaccac ccgattcagc
     3901 cagcagcaga tcgagatccc cgaggacctg ccggaggacg tctatgtcac gttcgcccag
     3961 caggacgccg tccgcgtcga ttgccacgac ggccgggtgc ggctgaccat ccggatcgag
     4021 gagctggagc aagaaggcac gcggaaccgc tggaccaact tcactgtccg tggctattac
     4081 ggtcccaacg cggaccagct cgacgcgaac ctcgtccgcg agggaatcat cgagttgatc
     4141 ggcgacaagc ggcagctgac gatcggccag cagctggccc tgcgggcgat cttcaacaag
     4201 gtgctgtcga agaaccgcaa gctgagcatc attaa
//
LOCUS       DASRJB010000007        10030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2823353, whole genome shotgun sequence.
ACCESSION   DASRJB010000007 DASRJB010000000
VERSION     DASRJB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823353"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..2692)
                     /locus_tag="VFB80_00155"
     CDS             complement(113..2692)
                     /locus_tag="VFB80_00155"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZN32186.1"
                     /translation="MSAARSMNVALSALSKPPARPLLADYRSPAGAFDEMLVAGGEIR
                     PAWQPLIRALGEIDRAALADRWEQARRVIRENGVTYNVHGDPAGMSRPWELDALPLLV
                     PAAEWNAIAAGLAQRALLLNLILADLYGPQRLLAEGLLPPELVFLNPGYLRPCHGLAV
                     PKSRYLYLYAGHLARDEAGSWVVLADKARGPTGAGYAIENRIVISRMLPDIFRDCQVQ
                     RLASFFLTMRDVLVDLAPAARGMPRIVLLSPGPTSPTYFEDAYLARYLGYALVEGGDL
                     TVRDNQVFLKTLGGLLKVDVILRRLRDEDCDPLELRGDSLHGVSGLTHAARCGQVVLV
                     NALGCSLLESPAVIPFLPTLARFFLREDLLLPSVPTWWCGDEAARRYVLENLEQLVIK
                     PSFPRPESGPIYGARLTAAQREELRGRIRQQPQDYVAQELVAGSTAPMWNGGQLRPTH
                     VAVRTFAFAAHDKYEVMPGGLARVSLSPDALGESALGGQGSKDVWVQSEGPVAAVTLL
                     HPAGAPVGLQRSGNDLPSRVADHLFWLGRHAERTEGAARLVRSVIARLTSETAFSSPQ
                     SLAALLRALAEQGRVRPEVVENATGQPVAQLERELLTFIFDAHRASSLAGMLADLQRA
                     AAVVRDRISIDSWRILNGLALGLAPPPTPDAEVALADVLSALNHLILDLSAFSGMGTE
                     SMTRGPGWRFLDMGRRIERGQHALALLRSTLVEPEADEDPVFEALLEIADSSMTYRNR
                     YLTNLQLPPLLDLLLTDESNPRSVAFQAAALTAHVDALPRGATQLLLGSEQRIALALL
                     TELRLTDVYSILQQGASGRRADLEEFLDRAATQLRQLSDEVTKHYLIHAGVSRQIGEI
                     RPS"
     gene            2863..3633
                     /locus_tag="VFB80_00160"
     CDS             2863..3633
                     /locus_tag="VFB80_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32187.1"
                     /translation="MFVSGSTNKWSAFLLSACVPGAGQLAAGSWTCLAWFLAAGLLAA
                     GLLTIERFNANSAVWVLPLKAGCGIGLCLLSAEHAKRLLETRPQAGRSTVVAGACRSH
                     SRGRAMHVEISLDMARGCEELWRLVSDLPRFLVIDPFHDRVTLMRDRPGLGVDLLLSH
                     NAFGLRFLRFGRIVAWHEGSGYTFTDFSSRGTASGFPHVFIVHMQPVIGGQGGAPLTR
                     LSIHIRGRWTSPLAPAWLGRLWVWLVCREHARLLRKAL"
     gene            3635..4798
                     /locus_tag="VFB80_00165"
     CDS             3635..4798
                     /locus_tag="VFB80_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32188.1"
                     /translation="MTSAAQTEAASVRMYCFRCGGELPVQGRPAVCPQCGLQYDPGRP
                     ETVATHRTSSRWKFWLPGFLASVLVGTLTYAGCLQIGGEMGWSLFFAVPVSFGALLGY
                     ACRLQTWGYVVLGILAITSVIFLLITFSLAGMFCGFTLGIIFLIPTFAGLVCGVILRV
                     VLIAGRWDHAWYFRWFVWLLAALPLAGQLIENRFPHRREIAVIQTGLTIDATPQEAWN
                     AIMFYEDVQHPPPWLLHLALPKPIRSQGDKQKPGEVVTCFYDCGEIKKRISQVEPPRR
                     LAFDVVSVRMRSENYANLKDGGFEISPVGDKQSRITLTTRFERKLRPAFIWEPIERRV
                     IHTLHGHVLEGMRRKAEGPQADDKPPPPYEPRDSKLYLPPLATAMPANIEAGR"
     gene            4795..5175
                     /locus_tag="VFB80_00170"
     CDS             4795..5175
                     /locus_tag="VFB80_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32189.1"
                     /translation="MNSEQSPAADRRPAGSQFSLRGLFALLTATSVILAILALVIKSP
                     LHWLGALFVPVICITLIGVMELGRRLFPPPAKRYLSPKDLATLPGVPFPPPRPTDYAD
                     GGCPFGPMPAAGPESANVDKAKPE"
     gene            complement(5179..6192)
                     /locus_tag="VFB80_00175"
     CDS             complement(5179..6192)
                     /locus_tag="VFB80_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZN32190.1"
                     /translation="MLSRVADSIFWMSRYIERAENVARFINVNLKLSIDLGPATLGQW
                     APLVYTTGDQDAFAERFDEPDQKKVIQFLAFDPENPNSIISCLRAARENARTVREMIS
                     SLMWEELNKFFLNVRAATTDQSVLAQPYDFLQQVILSSHLMDGITDATMSHNEAWHFF
                     VMGRLLERADKTSRILDVKYFTLLPSVGDIGSAVDTIQWSALLESASALEMYRKRHGR
                     ITPANVADFLMLDRDFPRAMHHCLIQAEESLTAITGNRGGRNHTSAEQKLGRLRSDLN
                     YARIDEIIQSGLHEFIDQFQVRLNEVGAAVSETFLAPQPLGTAPTQSQNQSQIQSAGM
                     SKE"
     gene            complement(6327..7808)
                     /locus_tag="VFB80_00180"
     CDS             complement(6327..7808)
                     /locus_tag="VFB80_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZN32191.1"
                     /translation="MDFQSYQTEGFYDELLTPELQPWPCGELLVKRLAALPTTELIRR
                     QRAAEMALLNMGITFAVYGHEAGTEKIWPFDIIPRIIDGREWDQIESGLKQRIHALNL
                     FIDDMYHERRIIRDGAVPQWLIDSARNYLPQCQGMDPPKGVWCHITGTDLVRDSDGRI
                     YVLEDNLRCPSGVSYVLENREIMKRTFPQSFDGFSILPVENYPDKLLEMLIHIAPAGV
                     SDPNVVVLTPGIYNSAYFEHCFLARQMGVELVQGSDLVVSDNTVYMRTTQGLERVDVI
                     YRRIDDDFLDPAIFRPDSTLGVKGLMDAYRAGKVALANAPGTGVADDKAVYAFVPKMI
                     KYYLGEETILPNVPSFVCGDEKQCQHVLANIDQLVVKPANESGGYGILLGPRATHAER
                     ERHKELIRANPRNYIAQPMLALSRVPTIVDDHFEGRHVDLRPYILYGKEIFVLPGGLT
                     RVALRKGSLVVNSSQGGGSKDTWVLRPDAALAARANGRPQGAA"
     gene            complement(8007..8321)
                     /locus_tag="VFB80_00185"
     CDS             complement(8007..8321)
                     /locus_tag="VFB80_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32192.1"
                     /translation="MRHLIALGLLAAGTMIFGCCGSVFLLATLLMPGPDAGSQSAGFA
                     PYEGGYQSPDGFYAEPGYAPAGGWHGGHEWSGRLSSGTVDPTGQGNDVISVDGEVLTF
                     PN"
     gene            complement(8460..8873)
                     /locus_tag="VFB80_00190"
     CDS             complement(8460..8873)
                     /locus_tag="VFB80_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32193.1"
                     /translation="MGWWKIDRATGQPIAGEHSALSRPPELVLPNAVPSVDDDEADCY
                     LGDGPGDMGSGLPAEFEAAIGDGRSLTDQQLRDCLLGRVVPQSLADRRPQLLQLVEAF
                     WLDIDGCYEDEWGRPARPAEKRWICEDVIERRRHK"
     gene            8955..9914
                     /locus_tag="VFB80_00195"
     CDS             8955..9914
                     /locus_tag="VFB80_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32194.1"
                     /translation="MPLTVQCTCGKSYQVDEALAGRRVKCKACGGALTIPQKPALLAA
                     AADDEFRLAPLDDGPKTAVPLAAPAPAAMKAAALPAATPAAKAPAPQSAAAAAPSGSL
                     PFFLAVSRGWSNTLIYRAYVDQGALLLIDLSPHNIMIDLEVARHADGSHWGVKTVQSL
                     KTWMISSIAVVGSILGLLGIGIARAGFQRPNEALQLASFLFTVAAFAIPLLILIATWT
                     MRTLVRRSQELDRLNADDLRRECGKGGNKYRLAAAEISELRVKPPAHPGGTNSQLAEA
                     TFKHPSGSWKLLLFAPQDVQLAVATFRRLLGGKVEVDPALERI"
BASE COUNT         1641 a         3273 c         3356 g         1760 t
ORIGIN      
        1 tgggcatcga ccacacgcgg ctgacgttca agttccaggg acggcactac cggctgacgg
       61 acgtgcacgg gcggatcgtg cgggaaatcc tggcgtaacc ggctgccgcg cgtcacgacg
      121 ggcggatttc gccgatctgc cgcgagacgc cggcgtggat caggtagtgc ttcgtcacct
      181 cgtccgagag ctgccgcaat tgtgtggcgg cgcggtcgag gaactcttcg agatccgccc
      241 gccggccgga ggcgccctgc tgcaagatgg agtaaacgtc ggtcagccgc agctcggtga
      301 gcagggccag ggcgatccgc tgctcgctgc ccagcaggag ctgcgtcgcg ccgcgcggca
      361 aggcatcgac gtgcgcggtc agcgctgcgg cctggaacgc aaccgagcgc ggattcgatt
      421 cgtcggtcag cagcaggtcc aagagcggcg gcagctgcag gttcgtgagg tagcgattgc
      481 gataggtcat cgagctgtcg gcgatttcca gcagcgcctc gaagacggga tcttcgtcgg
      541 cttcgggctc gacgagcgtg ctgcggagca gggccaaggc gtgctggccg cgctcgatgc
      601 ggcgacccat gtcgaggaac cgccagccgg gaccgcgggt catgctctcg gtgcccatgc
      661 cgctgaacgc cgagaggtcg aggatcaggt ggttgagtgc cgagaggacg tcggccagcg
      721 cgacctcggc gtcgggtgtg ggcggcggag ccaggccgag cgccaggccg ttgaggatcc
      781 gccagctgtc gatcgagatg cggtcgcgca cgacggccgc cgcccgctga agatcggcga
      841 gcatgccggc caggctgctc gcgcggtgag cgtcgaagat gaaggtgagc aactcgcgct
      901 ccagctgggc cacgggctgc ccggtggcat tctcgaccac ttcgggtcgc acgcggccct
      961 gctctgccaa ggccctgagc agcgccgcca ggctctgcgg gctgctgaaa gccgtttcgc
     1021 ttgtcagccg ggcgataacg ctccgcacca ggcgggcggc cccttcggtc cgctccgcgt
     1081 gccggcccag ccagaacagg tggtcggcca cgcggctggg caggtcgttt ccgctccgct
     1141 gcaagccaac gggcgcgccc gccggatgga gcagcgtcac cgccgcgacc ggcccttcgc
     1201 tctgcaccca gacgtccttg ctgccctgtc cgcccagggc cgattcgccg agggcatcgg
     1261 gcgagagcga gacgcgggcc aggccgccgg gcatgacttc atacttatcg tgcgcggcga
     1321 acgcgaaggt gcggaccgcc acgtgcgtcg gccgcaattg cccgccgttc cacatcggcg
     1381 cggtcgagcc ggcaaccagt tcctgcgcga cataatcctg cggctgctga cgaatccgcc
     1441 cgcgcagctc ctcgcgctgc gccgcggtca gccgagcacc gtaaatcggc cccgactccg
     1501 ggcgcgggaa gctcggctta atgacgagct gctccaggtt ttccaaaacg tagcggcggg
     1561 ccgcttcgtc gccgcaccac caagtcggaa ccgaggggag gagcaggtcc tcgcggagga
     1621 agaagcgggc cagcgtcggc aggaacggaa tcaccgcggg cgattcgagc aggctgcagc
     1681 ccagggcgtt gacgagcacg acctggccgc agcgggcggc gtgcgtcagt ccggagacgc
     1741 cgtgcagcga gtcgccgcgc agctccagcg ggtcgcagtc ctcgtcgcgg agccggcgca
     1801 ggatcacatc gaccttgagc agtccgccca gcgtcttcaa aaagacctgg ttgtcgcgaa
     1861 cggtcagatc gccgccttcg accagggcgt agccgagata ccgggccaga taggcgtctt
     1921 caaaataagt cgggctggtc gggccggggc tgagcaggac gatccgcggc atcccgcggg
     1981 cggccggcgc gaggtccacc agcacgtcgc gcatcgtgag aaagaagctg gccagccgtt
     2041 ggacctggca atcgcggaag atgtcgggca gcatccgcga gatgacaatg cggttctcga
     2101 tggcatagcc cgcgcccgtc ggcccgcggg ccttgtcggc caggacgacc cagctgccgg
     2161 cctcgtcgcg ggccaagtgg ccggcgtaga gataaaggta gcgcgacttg ggcaccgcca
     2221 gtccatggca cggacgcaga tagccggggt tgagaaagac cagctccggt ggcaagaggc
     2281 cctcggccag caggcgctgc gggccgtaca gatcggcgag gatcagattc agcaacagcg
     2341 cccgttgcgc gaggccggcg gcgatggcgt tccattcggc ggccggaacg agcagcggga
     2401 gggcgtccag ctcccagggc cgcgacatgc cggcggggtc gccgtgcacg ttgtaggtga
     2461 cgccgttctc gcggatcacg cggcgggcct gctcccagcg gtcggccagg gccgcgcggt
     2521 cgatctcgcc cagggcccgg atcagcggct gccaggcggg gcggatctcg ccgcccgcca
     2581 cgagcatttc gtcgaaggcc cctgccggcg agcggtaatc ggcaagcagc ggccgcgcgg
     2641 gcggtttgga aagggcggac aaggccacgt tcatagagcg tgcggcggac atccatgacc
     2701 tgcggcgagg gagcgagcgt cgtgcgctgc ttgtgccagc ataacgagca tccgccgcgg
     2761 gcgacaatgg atcatcggct gcacagtttg ggctccgctt ggtttgggac gacaagttat
     2821 cataactcgc ggcctgcggt cttgatttgc agcgagtcgc cgatgttcgt ttctggaagc
     2881 accaacaagt ggtcggcctt cctgctctcg gcctgcgtgc cgggggcggg gcagctggcg
     2941 gcgggaagct ggacctgcct ggcctggttt ctcgcggccg ggctgctcgc ggcggggctg
     3001 cttacgatcg agcggttcaa cgccaactcg gccgtatggg tgctgccctt gaaagccggc
     3061 tgcggcatcg gcttgtgcct gctaagcgcg gaacacgcca agcggctgct ggagacgcgt
     3121 ccgcaggctg gccgctcgac cgttgtggcg ggggcctgcc gatcgcacag ccgcgggcgg
     3181 gcaatgcacg tcgagatctc gctcgatatg gctcgcggat gcgaggagct gtggcggctg
     3241 gttagtgact tgccgcggtt tctggtgatc gatccgtttc acgatcgagt gacgctgatg
     3301 cgtgaccggc ctggtttggg cgtggacctg ctcttgtcgc acaatgcctt cgggctgcgg
     3361 tttttgcggt tcggccgcat cgtcgcctgg cacgaaggga gcggctacac gtttaccgac
     3421 ttcagcagcc gcggcacagc gagcggtttt ccgcacgtgt tcatcgtcca catgcagccg
     3481 gtaattgggg gccaaggcgg cgcgccgcta actcggttgt cgatccacat ccgcggccgg
     3541 tggacctcgc cgctcgcgcc ggcctggctg ggaaggctgt gggtctggct ggtatgccgc
     3601 gagcacgccc gactgctgcg aaaggcactc tgacatgaca tcagccgccc aaaccgaggc
     3661 agccagcgtg cggatgtact gcttccgctg cggcggcgag ctgccggttc agggccggcc
     3721 ggccgtctgc ccgcagtgcg gcttgcagta cgatccaggc cgcccggaaa ccgtggccac
     3781 gcaccgcacc agctcgcggt ggaaattctg gttgccgggg tttctggcgt ccgtgctggt
     3841 cggcacgctg acgtatgccg gctgcctgca gatcggcggc gaaatgggct ggtcgctgtt
     3901 cttcgccgta ccggtcagct ttggggcgct cttgggctat gcctgccgcc tgcaaacgtg
     3961 gggatacgtc gtgctgggta tcctggcgat cacctcagtc atctttctgc tgatcacgtt
     4021 cagcctggcg ggcatgttct gcggattcac gctggggatc atctttctca ttcccacgtt
     4081 cgcaggattg gtgtgcggcg tgatcctgcg cgtggtgctg atcgccggcc gctgggacca
     4141 cgcctggtat ttccgctggt tcgtgtggct gctggccgcc ctgccgctcg ccggccagct
     4201 gattgaaaat cgttttccgc accgccgcga aatcgccgtc attcagacgg gcctgacgat
     4261 cgacgctacg ccgcaggagg cgtggaacgc gatcatgttc tatgaagacg tgcagcatcc
     4321 gcctccctgg ctgctgcacc tggcgctgcc caagcccatt cgcagccagg gcgacaagca
     4381 aaaaccgggc gaggtcgtca cctgcttcta cgactgcggc gaaatcaaaa agcgaatcag
     4441 tcaggtcgag ccgccgcggc ggctggcgtt cgacgtcgta agcgtccgca tgcgaagcga
     4501 gaactacgct aacttgaagg acggcggctt cgagatttcg ccagtcggcg acaaacagtc
     4561 gcggatcacg ctgacgacgc gttttgagcg gaagctgcgg ccggcgttca tttgggagcc
     4621 gatcgagcgg cgggtgattc acacgctgca cggccacgtg ctcgagggaa tgcgccgcaa
     4681 ggctgagggt ccgcaggcgg acgacaagcc gccgcccccg tatgagccgc gggacagcaa
     4741 gctttatttg ccgccgctgg cgaccgcgat gcccgcgaac atcgaggccg gccgatgaat
     4801 tccgagcaat cgccggccgc cgatcgccgt cccgccggga gccagttctc gctgcgcgga
     4861 ctgtttgccc tgctcacggc aacgtccgtg atcctcgcca tcctggcgct ggtgatcaag
     4921 tcgcccctgc actggctggg tgctctgttc gtcccagtca tttgcatcac gctcatcggc
     4981 gtgatggaac taggtcgccg tctgtttccg ccgccagcga agcgctacct ctcgccgaaa
     5041 gacctggcga cccttccagg cgtcccgttc ccgccaccgc ggcccaccga ctacgccgac
     5101 ggtggttgcc cgtttgggcc catgccggcc gctgggccag aaagcgcaaa tgtcgataag
     5161 gcaaagcccg agtagtggtc actcctttga catgcccgct gactgtattt gcgactggtt
     5221 ttgcgattga gtcggcgccg tgcccagcgg ctgcggagcc aagaacgtct cgctcaccgc
     5281 ggcgccgact tcgttcaggc gaacctggaa ctggtcgatg aactcgtgca ggccgctctg
     5341 gatgatctcg tcgatgcggg cgtagttcag gtccgagcgg aggcggccga gcttctgctc
     5401 ggcggacgta tgattgcggc cgccccggtt gccggtgatg gccgtcagcg attcctcggc
     5461 ctggatcaga cagtggtgca tggcccgcgg aaaatcgcgg tcgagcatca ggaaatcggc
     5521 gacgttcgcg ggcgtgatcc tcccgtgccg cttgcggtac atctccaggg cgctggccga
     5581 ttcgagcagc gccgaccact ggatcgtatc gaccgccgag ccgatgtcgc cgacgctcgg
     5641 cagcagcgtg aagtatttta cgtcgaggat gcggctggtc ttgtcggccc gctccagcag
     5701 gcggcccatg acgaagaagt gccaggcctc gttgtgcgac atcgtggcgt cggtgatgcc
     5761 gtccatcagg tggctgctga ggatcacctg ctgcaggaag tcgtagggct gggccagcac
     5821 gctctggtcg gtggtcgcgg cccgcacgtt gaggaagaac ttgttcagct cctcccacat
     5881 cagcgacgag atcatctcgc ggacggtgcg ggcgttttcg cgggcagccc gcaggcagga
     5941 gatgatcgag ttggggtttt ccgggtcgaa agccaggaac tggatgactt tcttctggtc
     6001 gggctcgtca aaacgctcgg cgaaggcgtc ttgatcgccc gtcgtataga ccagcggggc
     6061 ccactggccg agggtagccg ggcccaggtc gatcgacagc ttcaggttga cgttgatgaa
     6121 tcgcgcgacg ttctccgccc gctcgatata ccggctcatc cagaagatcg agtccgcaac
     6181 gcgactgagc atcgttattc cgcctcgtgg cctctcgttc ccagggtcct ccctgggaac
     6241 gcactcctgg aggctcccgc ctcccgtccg cccgcgaggc aggagcctcg cggccgtgcg
     6301 gtcccaggct ggagcctggg accgagttac gccgcgcctt gcggccggcc gttggcccgc
     6361 gcggccagcg ccgcgtcggg ccgcaagacc cacgtgtcct tgctgccgcc gccctgcgac
     6421 gagttcacca ccagcgaacc ttttcgcagc gccacccgcg tcaaaccgcc gggcagcacg
     6481 aaaatctcct tgccgtagag gatgtacggc cgcagatcga cgtgccgtcc ttcgaagtga
     6541 tcgtccacga tcgtcggcac ccgcgacagc gccagcatcg gctgggcgat atagttccgc
     6601 ggattggccc ggatcagctc cttgtgccgc tcgcgctcgg cgtgcgtcgc ccgtggtccg
     6661 agcaagatgc cgtatccgcc cgattcgttc gccggcttga ccaccagctg gtcgatattg
     6721 gccagcacgt gctggcattg cttctcgtcg ccgcagacga acgacggtac gttaggcaag
     6781 atcgtctcct cgccgaggta gtacttgatc atctttggca cgaaggcgta gaccgccttg
     6841 tcgtcggcca cgcccgtccc cggtgcgttc gccagggcga ccttgcccgc gcggtaggcg
     6901 tccatcaggc ctttcacgcc gagcgtcgaa tcggggcgga agatcgccgg gtcgaggaag
     6961 tcgtcgtcga tgcggcggta gatcacatcg acccgctcca gcccttgcgt cgtccgcatg
     7021 tagaccgtgt tgtcgctgac gaccaggtcc gagccctgca ccagctccac gcccatctgc
     7081 cgggccagga agcagtgctc gaagtaggcc gagttgtaga tgcccggcgt gagtaccacg
     7141 acgttcggat cgctgacgcc cgccggagcg atgtggatca gcatctccag cagcttgtcg
     7201 ggatagttct cgaccggcag gatcgagaag ccgtcgaagc tctgcggaaa cgtccgcttc
     7261 atgatctcgc ggttttccag gacgtacgac acgcccgaag ggcaccgcag gttgtcctcc
     7321 aggacgtaaa tccgaccgtc gctgtcccgc accaggtccg tccccgtgat gtggcaccag
     7381 acgcctttcg gcgggtccat gccctggcac tgcggcaggt agttcctggc cgaatcgatc
     7441 agccactgcg gcaccgcgcc gtcccggatg atccgccgct cgtgatacat gtcgtcgatg
     7501 aacaggttga gcgcgtggat ccgctgcttt agcccgctct cgatctggtc ccactcgcgg
     7561 ccgtcgatga tccgcggaat gatgtcgaac ggccagattt tctcggtccc cgcttcgtgc
     7621 ccgtagacgg cgaaggtgat gcccatattc aggagggcca tttccgccgc ccgctgccgg
     7681 cggatcaact cggtcgtcgg cagcgccgcc agacgcttca ctagcaattc gccgcaaggc
     7741 cacggctgca gctcgggcgt gagcagctcg tcgtaaaagc cttcggtctg gtacgactga
     7801 aaatccatgc ttgaaccggc gcgagattcc aggcggctgc ccaataaccc ggcaccgcca
     7861 gggcgcacct tgggcaacgc aggcgtccgc gggccgagta aaggatggtt ttagcgactg
     7921 ggcaatcgac atgccatgcg gctggtctgc gcggcaaatg cgccacggat gaaacacaga
     7981 tgaaacacgg ggcaaatccg agtttcttag ttcggaaatg tgaggacttc gccatcgacg
     8041 ctgatgacat cgttgccctg gcccgtggga tcgacggtgc cgctgctgag gcggccgctc
     8101 cactcgtggc cgccgtgcca gccgcccgcg ggggcatagc ccggttctgc atagaagccg
     8161 tcaggacttt gatagccacc ctcataggga gcaaaaccgg cggattgcga gccagcatcg
     8221 ggccctggca tcagcaaggt cgccagcagg aacacgcttc cgcagcaacc aaaaatcatc
     8281 gtgcccgccg ccaacaggcc aagcgcaatg aggtgacgca tgtaccgact cctttttgat
     8341 ggaaggcgcg caaacggtgc acacctttca gcgcaaaaag gcccggctct gttacagccc
     8401 acagattcta tgcgaggcgg atggatgagg cgcgtaccgc ctgattcgtc acgccgcggt
     8461 cacttatggc gccggcgctc gattacatct tcacaaatcc aacgcttctc cgcgggccgc
     8521 gctggccggc cccactcgtc ttcgtagcag ccgtcgatgt ccaaccagaa tgcctcgacg
     8581 agctggagga gctgcggccg ccgatcggca aggctttgcg gtacgacccg ccccagcagg
     8641 caatcgcgga gctgctggtc ggtaaggctt cggccgtcgc cgatcgcggc ctcgaattcc
     8701 gcgggcaggc cagagcccat gtccccaggg ccgtcgccca aatagcaatc cgcctcgtcg
     8761 tcgtccacac tcgggaccgc gttcggcagg acaagttccg gcggccggct caaagccgag
     8821 tgctcacccg cgatcggctg gcccgtcgca cggtcgattt tccaccagcc catggctttc
     8881 gctccgatcc atttttgggg cgtttgccgt ctgctattct tcagtgtcgc tggctcgcca
     8941 gctgggaggc ccccatgccg ctcaccgtcc aatgcacctg tggaaaatct tatcaggtgg
     9001 acgaggcgct cgccgggcgg cgagtgaagt gcaaggcctg tggcggggcg ctgaccattc
     9061 cccagaagcc ggccctcctg gccgcggccg cggacgatga attccggctc gcgccgctcg
     9121 acgacgggcc gaaaaccgcc gtgccccttg ccgcgcctgc tcccgcggcg atgaaggccg
     9181 cggcgcttcc cgctgcgacg ccggccgcca aggcccccgc cccccagtcc gcggccgccg
     9241 ccgcgccttc cggcagcctc ccattctttc tggccgtgag tcggggctgg tcgaatacat
     9301 tgatctaccg agcttacgtc gatcagggcg ccctgctgct catcgatctc agtccccata
     9361 acatcatgat tgatcttgag gtcgcccgcc atgcggatgg ttcgcactgg ggcgtgaaga
     9421 ccgtacagtc cctgaagacc tggatgatca gctcgatcgc ggtggtcggc tcgatcctcg
     9481 ggctgctggg aatcggcatc gcccgcgccg gctttcaaag gccgaacgaa gcgctgcaat
     9541 tggcctcgtt cctgttcacg gtagcagcgt ttgcgattcc gctcttgatc ctgattgcga
     9601 cctggaccat gcgcactctg gtgcgccgca gtcaggagct ggatcgcctg aatgcggacg
     9661 acttgcggcg cgagtgcggc aaagggggca acaaatatcg cctcgccgcg gcggagatat
     9721 cggagctgcg cgtgaaacca ccggcgcatc ccggtggcac caacagtcaa ctggccgagg
     9781 cgactttcaa gcatccctcc ggaagctgga agctgctgct gtttgccccg caggatgtgc
     9841 aactggccgt cgccacgttt cgccgcctgc tcggaggcaa ggtcgaggtc gatccggcgc
     9901 tggaaagaat ctgaacatga gcaaggtcca gcagcagttc gagcaggccc agcgatttct
     9961 ggcgggcggc gtcagcagct cgacgcgcgt gaatcaggcg ctgggtcatc ctctgtactt
    10021 tcagcgggcc
//
LOCUS       DASRJB010000008        11017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1412358, whole genome shotgun sequence.
ACCESSION   DASRJB010000008 DASRJB010000000
VERSION     DASRJB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11017
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412358"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFB80_00200"
     CDS             <1..345
                     /locus_tag="VFB80_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN32195.1"
                     /translation="NRCRTALSGRKRRPVAEPSVEWVADDAPDQRAAQQLAEEVHLAL
                     SAVRPEYRQAFVLFHDRELSYAEIAVAMEVPLGTVKTWVHRARRELINLLRNRGAVPP
                     ERSVSQHAVPRV"
     gene            326..1024
                     /locus_tag="VFB80_00205"
     CDS             326..1024
                     /locus_tag="VFB80_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32196.1"
                     /translation="MQCQEFEDRWNELLDERGCPQADPRLAAHAGECESCRGLLTGGS
                     LLLRGLTQLPIPPLGREFARRVVTQVASDRESSVTARPASRAWLAVGVLLASAAAALL
                     AISLVWYARRGGDHVAGGGNRPAVVNRSAPGASPRRRGPAYAFGGQLLLSAPRLPSFQ
                     NYRGAIDNLAVTLPQTVEQLDQMERLAPGIRPVRLSLAMFWQTLRRSIPGSRSEEPAH
                     PRTTSWPHFGGLVA"
     gene            complement(1034..2137)
                     /locus_tag="VFB80_00210"
     CDS             complement(1034..2137)
                     /locus_tag="VFB80_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32197.1"
                     /translation="MPGSHDEPRFLVVRHARRKRHFYDVVLNWVAERFPRLRALFDVW
                     DLPDETARDFPHRPDLLVWRRLTALTKRLIRRDTTPLRLLRSGIPDGSDYRLHLAWLQ
                     DPVEQWSQATYARACRLAAECHSRGVPVVNRVDRLANAAKSTAARLMSAAGIRTPRMA
                     VIRDAAEFRETRLDISLPLFVREDWGHCGRMWRADTPAEIRRLPVSRLKRPVAVEIVD
                     VRDPRDGLFRKYRYVAAGEIGVSHHVQASSDWITRGDNRVVTEATRAEELDYISRPDP
                     HHVQLQRARRALGLEMVAFDYGLTQEGELILWEANPFPHIKISKRGLAYRNTALHRTL
                     LALLHLYLTTAGLPVPGEIADGLPYDVPHNRAA"
     gene            complement(2141..3121)
                     /locus_tag="VFB80_00215"
     CDS             complement(2141..3121)
                     /locus_tag="VFB80_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32198.1"
                     /translation="MKSAYFADAGPLWAITSFYNPSGYARRRHNYRLFRHHLQVPLLT
                     VELAYAGGFELADSDAEILVRCSGGDVLWQKERLLNVALERLPPTCTQVAWVDCDVLF
                     ERDDWAVVLSRQLERTPLVQPYSRALHLRRDAPLNRSPADGDILFIREAVAGLVARGA
                     SAADYLGHFGGQRRYKRSPGHVWAARREILQRHGLYDACITGGGDTAIASAAYGCPEE
                     VVNFQTLSAPHARHYRAWAESFHQSVRGEVGWLEGDLLHLWHGEAANRGVPQRYYDLA
                     SFDFEPEADIAISEDGCWRWNSDKPGLHAVLREYFAARKEDGAALRQGVG"
     gene            complement(3136..3483)
                     /locus_tag="VFB80_00220"
     CDS             complement(3136..3483)
                     /locus_tag="VFB80_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32199.1"
                     /translation="MTYYANASPLWAVTSYWARPLYQDVRAQIGFVSGDLIHLWHGDI
                     QDRGTFDRHRRFAEFEFNPAHDLALERSGCWRWNSDKPAMHQFVRDYFVSRHEDGRQP
                     AMPAGKQLESAAA"
     gene            complement(3499..4491)
                     /locus_tag="VFB80_00225"
     CDS             complement(3499..4491)
                     /locus_tag="VFB80_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32200.1"
                     /translation="MNPAAEAGPLWAITSYFNPVGYRRRRENYRVFHRQLAVPLVTVE
                     LSFSGRFELTPADAEILVQIQGRDVMWQKERLLNVGVEALPSSCTQVAILDCDVVFLR
                     PDWPQAISRKLEQFPLAQPFSTVYYIPRVVSWNDPQALACEYTRPSVAALIEAGGVGR
                     AVMGQFAGRRPGVRATGHAIAARRELLQEHGLYDANIIGSGDASLMAAALGLFDAALE
                     YQALNEAQRAYYLAWARPFSREVRGQIGCARGDLIHLWHGEMGDRGTLARHRSLAAFG
                     FDPQVDIALEPTGCWRWNSDKPAMHQFVRDYFATRYEDGRQPASGRAELLPAAA"
     gene            complement(4488..5204)
                     /locus_tag="VFB80_00230"
     CDS             complement(4488..5204)
                     /locus_tag="VFB80_00230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN32201.1"
                     /translation="MDPESLTEWIGLLFAHPDLARMGHAQRVADRNLGLGWLYYALAR
                     TIRPAAAVVIGSYRGFVPLVLGRALADNSEGGEVAFIDPSFVDDFWKDAAAVRRYFAG
                     LRVTNITHHLQTTQEFVRSPAYDELGSVGLVFVDGFHSAEQARFDFEAFADRLAPGGM
                     ILLHDSVWRMATGLYGSDRHYVHGVVDFVAELKRQPQWQVFDVPCGDGVTIVRQSAVP
                     PPPSRRRSAAATLLAAGAAP"
     gene            complement(5328..6494)
                     /locus_tag="VFB80_00235"
     CDS             complement(5328..6494)
                     /locus_tag="VFB80_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZN32202.1"
                     /translation="MNTVKSAAVIVVLLVVLYGVYVALNKPDFFSPPPAPADVAPPLV
                     EFGPTDPGPPHTVSPSLASGPPATSPADSTQRSVRGGEFQPSTDPASLPPPTSLPSTA
                     PPPDPTASAGADLRRSNFETPAATTTGSQAPPLDSAAAPPPAASAPPANYAALTAYSL
                     KRDFQEAEQLVADGKYRAALAKLSPYYNSADLTSADRGQLLAWLDALAAKVIYSREHL
                     LAAPYIVNKSQTLYDIANEYKVSWLVLHSINRQVRDPMVLVQGTELKIVPGPFRAEVN
                     LATSELTLFLGELYAGRFPFTIGDQPPRPGTYQVHDKRRDQTFYGLDGRVIPGNDPAN
                     PYGGYWIHLGGEVAIHGSAISGGAQPLGCISLSPKDAQDVNAILSLGSEVTIRR"
     gene            6704..7045
                     /locus_tag="VFB80_00240"
     CDS             6704..7045
                     /locus_tag="VFB80_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZN32203.1"
                     /translation="MGKTIFKRIIDKEIPAKLVYEDDRCLAFHDIGPQAPTHVLLIPK
                     QEIASLAAAADSDAALLGHLLVVARKLAEQLNLASGYRVVINCGPDGGQSVDHLHLHL
                     LGGRAMKWPPG"
     gene            complement(7123..7548)
                     /locus_tag="VFB80_00245"
     CDS             complement(7123..7548)
                     /locus_tag="VFB80_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32204.1"
                     /translation="MKALRIGLPAAALAMICSCNSKPEPAQLSGKVTFKGQPVPAGYI
                     TFTPDVAAGTLGQNKVMQIKDGVYDSAKESEPGINPGTYQIRIAGFDGIKIPFFGQGK
                     QIFNPIQDQFKVPDGKTTKDFVVPDSAGENVRIEKTADT"
     gene            complement(7582..8661)
                     /locus_tag="VFB80_00250"
     CDS             complement(7582..8661)
                     /locus_tag="VFB80_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32205.1"
                     /translation="MPRLSRGFTLVELLVVIAIIGVLVALLLPAVQSAREAARRMTCS
                     NNVKQLCLACHNFEDTFGTLPPWASSKAGEYGSCHFLILPYLEQNNLFQKANGNSFNV
                     RTEAVKVFCCPVDVTAPNGKFSNIAVNYPGNGTSVGRTSINGVVYGAATYAYNAWISP
                     ADPAATEAVMNNGHPEKSLLSLVKLKDGTSNTILFGERMAFCTGRDYPSATANPRLAA
                     GSVTWSIWARGGKNTTNANWNDGAPATNLPAAGSGAIGGYSWWDNPIFNHPYTGFPNS
                     PGPRSDSNFRQNWDGGVVNPGGIQAGAIPHRCDYRRLQALHSGGVMVAGLADGSVRNV
                     NATISALTFDRVCYPNDGQVIGSDW"
     gene            8915..9487
                     /locus_tag="VFB80_00255"
     CDS             8915..9487
                     /locus_tag="VFB80_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32206.1"
                     /translation="MNSDHHSPAAPAGDSAAGPQFARLLVRHDRALLRYIMTLLPRRD
                     DAEEVLQRTATVLWEKFAEYDREREFLPWALRVAYFEVLNFRKQIARDRLVFREDVME
                     QLAESRQSQAAQLEAQRRALALCLNELSDQDQALLRRRYADSETIAALADEQGKTAKA
                     LYRRLDRLREAIARCAERRLRAELSLETSQ"
     gene            9503..>11017
                     /locus_tag="VFB80_00260"
     CDS             9503..>11017
                     /locus_tag="VFB80_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZN32207.1"
                     /translation="MSEPISQSQAAERALFRRLVDDQLSPDEFAGLEQRLLADREFRL
                     RCVRYMDLEASLYEALSVPLSLPEEGAKGRSRRGRRWKMAVVGTVLAAAAVVLIAVFV
                     GRRTDRPGAEYLEAKLKGLEVAAIITHVEGLPADSAGRLKEGMRLKPGSLKIQQGQLQ
                     LEFLGGAKLLVAGPAELHILSPKSATLVSGRAATRVLQWGHGFVLNTPEAVMVDLGTE
                     FAVGVDELQGSSVQVVDGEVEVSVLGEDGSTLTSERLEQDGALRIRKGATTLEPDKAP
                     LPPLTIRDEPPRPLAVPESYVRTVLQAKPTLYWRFESLAGGVVPNEAGSLFAAELQMD
                     ASDPDAVRVGDGQLHLAQSAGPRFLATTEPIPKLNAKSYSVEMWVNPAMLHWATLMGI
                     VPEADAKSLTHLNVIELAHQTPLVHEPGAFRFMHRHPPEGKGGVNIFTASGCTPGRWH
                     HLVSVKTPNELRLYLNGQLTRRLDGTMGSDEAAYHVLLGELRATTPERQFVGSLD"
BASE COUNT         1874 a         3543 c         3785 g         1815 t
ORIGIN      
        1 aaccgctgcc ggacggcgct gtcgggccgc aagcggcggc cggtggccga gccgtcggtc
       61 gagtgggtcg ccgacgacgc acccgaccag cgggcggcgc agcagctggc cgaggaagtg
      121 catttggcgc tttctgcggt gcggcccgaa taccggcagg cgtttgtgtt gtttcatgat
      181 cgggagctga gttacgccga gatcgccgtc gcgatggagg tgcccctggg caccgtgaag
      241 acctgggtcc atcgcgcccg gcgggaattg atcaacttgc tgcgaaatcg cggcgcagtg
      301 ccgcccgaac gatccgtcag ccaacatgca gtgccaagag tttgaagacc gctggaacga
      361 gctgctcgac gagcgcggct gcccccaggc cgatccgcgc ttggctgcgc atgctggcga
      421 gtgcgaaagc tgccgcgggc tgctgacggg cggatcgctg ctgctgcgcg ggcttacgca
      481 gctgccgatt ccgccgctgg gacgggaatt tgcccgccgc gtcgtgactc aggtcgcgtc
      541 ggacagggag tcgtcggtca ccgcgcggcc cgcgtctcgc gcctggctgg cagtcggggt
      601 tttgctggcg tcggcggcag cggccttgct cgcgatttct ctcgtctggt acgcccgccg
      661 gggcggcgac cacgtcgccg gcggcggaaa ccgcccggcc gtcgtgaatc gctccgcgcc
      721 gggtgccagc ccgcggcggc gagggcccgc ctacgcgttc ggcggccaat tgctgctttc
      781 agcgccgcgg ctgcccagct ttcaaaacta tcgcggcgcg atcgacaacc tggcggtcac
      841 gctgccgcag actgtcgagc agctcgacca gatggagcgg ctggcccccg gcatccgccc
      901 cgtgcggctc tcgctcgcca tgttctggca gacactccgc cgcagcatcc ccggctcgcg
      961 cagcgaagag cccgctcatc cccgcacgac gtcctggccg cacttcggcg ggctggttgc
     1021 gtagctggga aactcacgcc gcacgattgt gcgggacgtc gtagggcagg ccgtcggcga
     1081 tttcgcccgg cacgggaagg ccggcggtgg tcaagtagag gtgcaggagc gccagcagcg
     1141 tgcggtgcag ggcggtgttg cgataggcga ggccgcgctt ggagattttg atgtgcggga
     1201 agggattcgc ttcccagagg atgagctccc cctcctgcgt caggccgtag tcgaaggcga
     1261 ccatctccag gcccagcgcg cggcgggccc gctgaagctg aacgtggtgt ggatcggggc
     1321 gcgagatgta gtcgagctcc tcggcccgcg tcgcctcggt aaccacgcgg ttgtcgccgc
     1381 gggtgatcca atcgctcgac gcctggacgt gatggctgac gccgatctcg ccggcggcga
     1441 cgtagcggta cttgcgaaac aggccgtcgc gtgggtcgcg gacatcgacg atctcgaccg
     1501 ccacgggccg cttcagccgg gacaccggca agcggcggat ttcggccgga gtgtcggccc
     1561 gccacatccg gccgcaatgt ccccagtcct cgcggacgaa cagcggcagg gaaatatcca
     1621 gccgcgtttc gcgaaactcg gcggcgtcgc gaatgaccgc catccgcggc gtgcggatgc
     1681 ccgcggcgga catcaagcgg gcggccgttg acttcgcggc gttggcgagc cggtccacgc
     1741 ggttgacgac gggaacgccg cggctgtggc attcggcggc cagccggcag gcccgggcgt
     1801 aagttgcctg cgaccactgc tcgaccggat cctgcagcca cgccaggtgc agccggtaat
     1861 cgctaccgtc agggattccg ctgcgcagca atcgcagtgg agttgtgtcg cggcggatga
     1921 gtcgtttggt cagcgccgtc agccgccgcc agacgagcaa atcgggacgg tgaggaaagt
     1981 cgcgggccgt ttcgtcgggc aggtcccaca cgtcgaacag cgcacgcagt cgcggaaagc
     2041 gctcggcgac ccagttgagc acgacgtcgt aaaagtgccg cttgcgccgc gcgtggcgga
     2101 ccacgagaaa tcgcggctca tcgtgactgc ctggcatcgg ctagcccact ccctggcgca
     2161 aggccgcgcc gtcttctttg cgggccgcga aatactcgcg cagcacggcg tgcagccccg
     2221 gcttgtcgct gttccaccgc cagcagccgt cttcgctgat tgcgatgtcg gcctcgggtt
     2281 caaaatcaaa cgacgccaga tcgtaatagc gctgcgggac gccgcgattg gcggcttcgc
     2341 cgtgccagag gtgcagcaaa tcgccctcca gccacccgac ctcgccgcgg accgactgat
     2401 ggaacgactc ggcccaggcc cggtagtgcc tggcgtgcgg ggcgctcagg gtctggaagt
     2461 tgacgacctc ctccgggcag ccgtaggccg cggaggcgat ggccgtatcg ccgccgccgg
     2521 tgatgcaggc gtcgtacagt ccgtgccgct gcaggatttc gcggcgggcc gcccagacgt
     2581 gcccgggcga acgcttgtag cgccgctggc cgccgaagtg gcccaggtag tcggccgccg
     2641 acgctccccg ggcgaccagt ccggccaccg cttcgcggat gaagaggata tcgccgtcgg
     2701 ccggcgagcg gttcaaaggc gcgtcgcgcc gcaggtgcag tgcccgcgaa tagggctgca
     2761 ccagcggggt tcgttccagc tggcggctga gcaccacggc ccaatcgtcc cgctcgaaca
     2821 gcacgtcgca atcgacccag gcgacctgcg tgcaagttgg cggcaagcgc tccagggcga
     2881 cgttcaagag ccgctccttt tgccaaagca cgtcgccgcc gctgcagcgg acgaggattt
     2941 cggcgtcgga gtcggcgagt tcaaatccgc cggcatacgc cagctcgacg gtcaacagcg
     3001 gcacctgcaa gtggtggcgg aacagacggt agttgtggcg acgccgcgca tagccgctgg
     3061 ggttgtagaa gctcgtgatg gcccacagcg ggccggcgtc ggcgaaatag gcgctcttca
     3121 tggatgtcga tcgattcagg ccgccgcgga ctctagttgc ttgccggctg gcattgccgg
     3181 ttgccggcca tcttcgtgcc ggctgacaaa atagtcgcgc acgaactggt gcatcgctgg
     3241 cttgtcgcta ttccaccgcc agcagccgga tctctccaag gcgagatcgt gggcgggatt
     3301 gaattcgaat tcggcaaagc ggcggtggcg gtcgaatgtg ccgcgatctt ggatatcccc
     3361 gtgccagagg tgaatcagat cgccgctcac gaagccgatc tgtgcgcgga catcctggta
     3421 cagcggccgg gcccagtagc tcgttacggc ccacagcggg ctcgcgttag cgtaataggt
     3481 cataagtcgc gtgttcgctc aggccgccgc tggcagaagc tccgcgcggc cgctcgcggg
     3541 ctgccggccg tcttcgtagc gggtcgcaaa ataatcgcgt acgaactggt gcatcgccgg
     3601 cttgtcgctg ttccaacgcc agcagccggt aggctccagg gcgatgtcca cctgcggatc
     3661 gaaaccgaag gcagccaggc tccgatgtcg cgccagcgtg ccgcggtcgc ccatctcgcc
     3721 gtgccagaga tggatcaagt cgccgcgcgc gcagcctatt tgaccgcgga cctcgcggga
     3781 aaagggccga gcccaagcca ggtagtaggc tcgctgcgcc tcgttgagcg cctggtattc
     3841 cagagcggca tcgaacaggc cgagcgcggc ggccatcaac gaggcgtcgc cactgccgat
     3901 gatgttggca tcgtacaggc catgctcttg caagagttcg cggcgggcgg cgatcgcatg
     3961 ccccgtggct cggactcccg gccggcggcc cgcaaactgc cccatgaccg cgcggccgac
     4021 gccccccgct tcaatcagcg ccgccaccga gggccgcgta tattcgcacg cgagcgcttg
     4081 cgggtcgttc caggacacga cgcgcgggat gtaataaacc gtcgagaagg gctgcgccag
     4141 cgggaactgc tccagcttgc gactgatggc ctgcggccag tcggggcgca ggaacactac
     4201 gtcgcagtcg aggatggcga cctgcgtgca gctggaaggc agcgcctcaa ccccgacgtt
     4261 cagcagccgt tccttttgcc acatcacgtc gcggccctga atctggacga ggatttcggc
     4321 gtcggccggc gtcagctcga agcggccgct gaacgacaac tccacggtca ccagcggcac
     4381 ggcgagctgg cggtggaaga cgcgatagtt ctcgcggcgg cggcgatagc cgacggggtt
     4441 gaagtagctc gttatggccc acagcgggcc ggcctcggcg gcgggattca tggcgcggct
     4501 cctgctgcaa ggagcgtagc ggcggcgctg cggcggcgcg aaggcggcgg cggcacggca
     4561 ctttgacgga caatcgtcac gccgtcgccg cagggaacgt cgaaaacttg ccactgcggc
     4621 tggcgcttca gctcggccac gaaatcgacg acgccgtgca cgtagtgccg gtcactaccg
     4681 tacaggccgg tggccatccg ccagacgctg tcgtggagca ggatcatccc gccgggcgcg
     4741 aggcgatcgg cgaaggcttc gaagtcaaaa cgggcctgct cggccgagtg aaatccatcg
     4801 acgaagacga ggccgacgct gcccagctcg tcgtaggcag gcgagcggac aaattcctgc
     4861 gtcgtttgca gatggtgcgt aatgttcgtg acacgcagcc cggcgaagta gcgccgcacc
     4921 gcggcggcgt ctttccagaa gtcatcgacg aacgagggat cgatgaaggc gacctcgcct
     4981 ccctcggagt tgtcggccag ggcgcggccc agaacgagcg gcacaaagcc gcgatacgag
     5041 ccgatgacga ccgcagccgc ggggcggatc gtgcgagcca gggcgtaata gagccagccc
     5101 agcccaagat tccggtccgc cacgcgctgg gcgtgtccca tccgcgccaa gtcgggatgc
     5161 gcgaagagca gcccgatcca ctccgtcagc gattcagggt ccatgctgcg tccttgcgcg
     5221 tttccgtcgc cgcctggccc caagcgaggg aaatcatgca atcttgcgcc gaaagcggca
     5281 aggtcagctt tttgctgcgg aaaatcttcg actcgccgcc ctgcggatca gcggcggatc
     5341 gtcacttcgg agcccagcga cagaatcgcg ttgacgtcct gggcgtcctt ggggctgagg
     5401 ctgatgcagc cgagcggttg cgcgccgccc gaaatggcgc tgccgtggat ggcgacttcg
     5461 ccgcccaggt gaatccagta gccgccgtag ggattggccg gatcgttgcc tgggatgacg
     5521 cggccgtcga gcccgtagaa agtctggtcg cggcgcttat cgtgcacctg gtaagtaccg
     5581 ggccgcggcg gctggtcgcc gatggtgaac ggaaagcggc ccgcatacag ctcgccgagg
     5641 aagagagtca actcgctcgt ggccaggttc acttcggcgc gaaacggtcc gggcacgatc
     5701 ttcagctcgg tcccctgcac gagcaccatc ggatcgcgga cctggcggtt gatgctgtgc
     5761 agcaccagcc acgagacctt gtattcgttg gcaatgtcgt agagcgtctg actcttgttg
     5821 acgatgtagg gcgcggccag caagtgctcg cgggagtaaa tcaccttggc ggccagggcg
     5881 tcgagccagg ccagcagctg gccacggtcg gcgctggtca ggtccgcgct gttgtaatag
     5941 ggcgagagct tcgccagcgc cgcccggtac ttgccgtcgg cgacgagctg ctccgcttcc
     6001 tggaagtcgc gcttgagcga ataggccgtc agggcggcgt agttcgccgg cggcgcgctc
     6061 gcggccgggg gcggcgcggc ggcggagtcc agcggcgggg cctggctgcc agttgtcgtg
     6121 gctgccggcg tttcgaaatt gctccgccgc aaatccgcgc cagcgctggc ggtgggatcg
     6181 ggaggcggcg cggtggaagg caggctggtg ggcggcggca gactggccgg atcggtgctg
     6241 ggctggaact cgccgccgcg tacgctgcgc tgcgtggaat cggcggggct ggtagcgggc
     6301 ggcccagacg ccagcgaggg ggagacggtg tgcggcggac cgggatcggt gggcccgaac
     6361 tcgaccagcg gcggcgcgac gtcggccggc gcggggggcg ggctgaagaa atcgggcttg
     6421 ttcagcgcga cgtaaacgcc gtacagcacg accagcagca cgacgatgac cgccgccgat
     6481 ttgactgtgt tcacgaccgt cctccttgac gagcacgctg gccttgggcg gcgattgtcc
     6541 gttcgcggcc cacccaggca ttcctggcag ccagagaatc gtgcaccgcg gatgatagat
     6601 cgacacggcc agtgcagcca gagcagtttt gcggctggta ggccccttcc ggccgcgggt
     6661 ttacaatcgg cgctgcggcg ggttcgctag cggagcagga gccatgggca agacgatctt
     6721 caaacggatc atcgataagg aaatcccggc gaagctggtt tatgaggacg accggtgcct
     6781 ggcgtttcac gacatcggcc cgcaggcccc gactcacgtc ctgctgattc ccaagcagga
     6841 gatcgcctcg ctagcagcgg cagccgacag cgacgcggcg ctcctggggc acctgctggt
     6901 cgtcgcccgc aagctggccg agcagctcaa tctcgctagc ggctaccggg tggtcatcaa
     6961 ctgcggcccc gacggcggtc agagcgtcga tcacctgcac ctgcacctgc tcggcggccg
     7021 cgcgatgaaa tggccgccgg ggtgagattc aaagccgcga ccagtggtcg cggtctaccg
     7081 aaacaccgcg accactggtc gcggctttgg gagacgtggg gcctacgtat ccgccgtttt
     7141 ctcaatcctc acgttttcgc cggccgagtc gggcacgacg aagtctttcg tcgtcttgcc
     7201 gtcgggcacc ttgaactggt cctgaatggg gttgaagatc tgcttgccct ggccgaaaaa
     7261 cgggatcttg atcccgtcga agccggcgat gcggatctgg taagtgccgg gattgatgcc
     7321 cggctcgctc tccttggccg agtcgtacac accgtctttg atctgcatca ccttgttttg
     7381 gcccagcgtg ccggcagcga cgtcgggagt gaaggtaatg tagcccgccg ggaccggctg
     7441 ccctttgaac gtaaccttgc cggagagctg cgccggctcc ggcttcgagt tgcagctgca
     7501 aatcatggcc agagcggcag ccggcagccc gatcctgagt gccttcatgt gcaagaacct
     7561 tcgaagcatg ggaagtgagc cttaccagtc gctgccgatc acctgcccgt cgttcgggta
     7621 acagacgcgg tcaaacgtca aggcgctgat agtggcgttc acattgcgga cgctgccatc
     7681 ggccaggccg gctaccatca cgccgccgct gtgcagcgcc tgcagccggc gataatcgca
     7741 gcgatggggg atcgccccgg cttgaatgcc gccgggattg accacgccgc cgtcccagtt
     7801 ctggcggaaa ttcgagtcgc tccgcgggcc gggcgagttg gggaagcccg tgtagggatg
     7861 gttgaaaatg ggattgtccc accagctata gccacctatc gcgcccgagc ccgcggcggg
     7921 aaggttggtg gcgggagcgc cgtcgttcca gttggcattc gtggtgttct ttccgccccg
     7981 agcccaaatg ctccaggtca ccgagccggc cgccagccgc gggttggccg tggcgctggg
     8041 gtaatcgcga ccggtgcaga atgccatgcg ctcgccaaac agaatcgtgt tgctggtgcc
     8101 atccttgagc ttcaccagcg atagcagcga cttctcggga tggccgttat tcatcacggc
     8161 ttccgtcgcc gccgggtcgg ccggcgaaat ccaggcgttg tacgcgtacg tcgcggcgcc
     8221 atacacgacg ccgttgatcg aagtgcggcc gaccgaggtg ccattgcccg gatagttgac
     8281 ggcgatgttg gaaaacttcc cgttcggcgc cgtgacatcc acggggcagc aaaagacctt
     8341 gactgcctcg gtgcgaacat tgaacgagtt tccgttagcc ttctgaaaga ggttgttctg
     8401 ctccaggtag ggcagaatca gaaagtggca ggagccatac tcccccgcct tgctgctggc
     8461 ccaggggggc agtgtgccga aggtgtcttc aaagttgtgg caggccaggc acagctgctt
     8521 gacgttgttg ctgcacgtca tgcggcgggc ggcctcgcgg gccgactgca cagccggcaa
     8581 cagcagcgcg accaggacgc cgatgatggc aatcaccacg agcaactcga ccaacgtaaa
     8641 cccacgggag aggcgtggca tgtggtcact ccgtcgcatt cttagagatg gatgaaaaga
     8701 caagccgccc cccaagcacc caccagcagc cccgattatt gcatcgccgc tggataggat
     8761 ctcccgcgaa tcagccaatt cccccaagaa acaatccaaa aaacgccaaa aatggttcct
     8821 tcggccccgt caggcgacaa atcaccgggt tttgatcctt gccgcgggga atccgcgcat
     8881 gccgtggaga cttaagatag gcgcatcctc tcccgtgaat tccgatcacc attcaccagc
     8941 ggcacccgcg ggcgattcgg cggctggtcc ccagttcgca cggctgctgg tgcggcacga
     9001 ccgggcgctg ttgcggtaca tcatgacgct gctgccgcga cgcgatgacg cagaggaagt
     9061 gctccagcgg acggcgacgg tgctgtggga gaaattcgcc gagtacgacc gcgagcggga
     9121 gttcctgccc tgggcgctcc gcgtggccta tttcgaggtt ttgaacttcc gcaagcagat
     9181 cgctcgcgac cggctcgttt tccgcgagga cgtgatggaa caattggccg aaagtcgcca
     9241 gtcgcaggcc gcgcagctcg aagcccagcg gcgggcgctg gcgctgtgcc tgaacgaatt
     9301 gagcgaccag gaccaggccc tgctccgccg ccgctatgcc gactcggaga ccatcgccgc
     9361 cctggccgac gagcagggca agaccgccaa ggctttgtat cggcgactcg accgcctgcg
     9421 ggaggcaatt gcccgttgcg ccgagcgccg gctgcgagcc gaattgtcgc tagaaaccag
     9481 ccaataaccc aggagccgcc ctatgagcga gcctatcagc caatcgcagg cagccgaacg
     9541 ggccctgttt cggcggctcg tcgatgacca gctttcgccc gacgaatttg cggggctgga
     9601 gcaacggctg ctggccgacc gcgaatttcg cctccgctgc gtgcgctaca tggacctgga
     9661 agccagcttg tacgaggcgt tatcggtccc cttgtcgctg ccagaggaag gggcgaaagg
     9721 ccggtcgcga agaggccgcc ggtggaagat ggcggttgtc ggcacggtct tggccgccgc
     9781 tgcggtcgtg ctgatcgcgg tcttcgtcgg acgccggacc gatcgccccg gcgcagagta
     9841 tctagaagcc aagctgaagg gcctggaagt cgccgcaatc atcacccacg tcgaggggct
     9901 gcccgccgat agcgctggcc ggctcaagga gggcatgcgg ctgaagcctg gctcgctgaa
     9961 aatccaacag ggccaattgc agctggagtt cctcggcggg gccaagctgc tcgtagccgg
    10021 cccggctgaa ttgcatatcc tctcgcccaa gagcgcgacg ctggtttccg gccgcgcggc
    10081 aactcgcgtg ctgcagtggg ggcacggctt tgtgctcaac acgcccgagg ccgtgatggt
    10141 cgatctgggc acggagttcg ccgtcggtgt cgatgagctg caaggcagct ccgtgcaggt
    10201 tgtcgacggc gaggtggaag tctcggtgct cggcgaggac ggcagcacgc tgaccagcga
    10261 gcggctcgag caggacggcg ccctccgcat ccgcaaaggg gcaaccacgc tggagcccga
    10321 caaggctccg ctgccgccgc tgaccattcg cgatgagccg ccgcggccgc tggccgtgcc
    10381 ggaaagctac gtccgcacgg tgctgcaagc caagccgacg ctctactggc ggttcgaatc
    10441 gctggccggc ggcgtggtgc ccaacgaagc gggttcgctg tttgccgcag aactgcaaat
    10501 ggacgcgagc gatccggacg cagtccgcgt cggggacgga caattgcacc tggcccagtc
    10561 cgccggcccg cgtttcctgg caacgaccga acccattccc aagctcaacg cgaagtcgta
    10621 ttcggtcgaa atgtgggtca acccggccat gctgcactgg gcgacgctca tgggcattgt
    10681 tccggaggca gacgccaagt cgctgacgca cctgaacgtc atcgagctgg cccatcagac
    10741 gccgctcgtc catgagcccg gtgcgttccg gttcatgcac cgccatccgc cggagggcaa
    10801 aggcggcgtg aatatcttca ccgccagcgg ctgcacgccg ggccgctggc accacctggt
    10861 ctcggtcaaa acgccgaacg agctgcggct atacttgaat gggcaattga cgcggcggtt
    10921 ggacggcaca atgggcagcg acgaggcggc ctaccacgtg ctgctgggag agctgcgggc
    10981 cacgaccccc gaacgtcagt tcgtcggctc gctcgac
//
LOCUS       DASRJB010000009         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3669457, whole genome shotgun sequence.
ACCESSION   DASRJB010000009 DASRJB010000000
VERSION     DASRJB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669457"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFB80_00265"
     CDS             <1..339
                     /locus_tag="VFB80_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZN32208.1"
                     /translation="AIVAMLETATGRSAFSVGKPSPVMMRAARKELGLATGETIMIGD
                     TMETDILGGVNLGYRTALVLTGSTRRDDLERYAYRPDLVLNSLADLNDEQAYQRAVGA
                     LAAEAASVVA"
     gene            complement(734..1168)
                     /locus_tag="VFB80_00270"
     CDS             complement(734..1168)
                     /locus_tag="VFB80_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32209.1"
                     /translation="MPMRRPRPVRRRRGVILMVVLSLLTLFAIVGISFVFQAQRTASA
                     EAAANWRQVREAAPIIERDLLEALAGETDVSGSYSALQGLDLTSSDLRAAICAAARAE
                     QDPKERRRLERLCDLADDLRRAISLLQWLLGQVEQQATFPPG"
     gene            complement(1212..1727)
                     /locus_tag="VFB80_00275"
     CDS             complement(1212..1727)
                     /locus_tag="VFB80_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32210.1"
                     /translation="MLRSVTVFVGAFLFLVGVAAAQQAAAPKAEPRPFTFTTLEILNG
                     DGELQRLLKQRYNAAGEDLEARMALYGAGRVSLEAICASLERFTKASLDLAATPVERV
                     NELDRALAAARSVEAIVEEKYKFEVEPVQAFKHARYVRLDLEIQLLRAREAAKLGAAG
                     SPAAEVAAAKR"
     gene            1944..2669
                     /locus_tag="VFB80_00280"
     CDS             1944..2669
                     /locus_tag="VFB80_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32211.1"
                     /translation="MSQSPSPEVRPGNILLPPLAGLGLGLAAALLAWVLIQRVHPVFL
                     MPENLHLSMGAPAAQVNAYRAKQRELDRHHAMLYLGSLGAFLGALLGVAEGRLRRSIL
                     PVATTLPLGALGGVAGGLLACLVQEQVRVNVGLAEIKHTVMAQLALCLPLAGCIGLGL
                     GLATRSLRGLVLAVLAGLAAGVLAGVLYPLITSTFLPGISTDSLLPEEASSRLMWLGL
                     LAGVIGLVVPIAGRRRKSATSNA"
     gene            complement(2753..>2988)
                     /locus_tag="VFB80_00285"
     CDS             complement(2753..>2988)
                     /locus_tag="VFB80_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HZN32212.1"
                     /translation="SCTPSNRLNPPCRNHPNPKPANPDPNNEESIGARSRHPTGVMAA
                     LCDGSVRFYSNNVALDSWRALGTAHGGDIISGE"
BASE COUNT          459 a         1023 c          989 g          517 t
ORIGIN      
        1 gcgatcgtgg cgatgctcga aacggcgacg ggccgctcgg cgttcagcgt cggcaagccc
       61 agcccggtaa tgatgcgggc ggcccgcaag gaactgggcc tggccaccgg cgagacgatc
      121 atgatcggag acaccatgga gaccgacatc ctggggggcg tcaacctcgg ctaccgcacc
      181 gccctcgtgc tcaccggctc gacccgccgc gacgacctgg agcgctatgc gtaccggccg
      241 gacctggtgc tgaactcgct cgccgacctc aacgacgagc aggcctacca gagggccgtc
      301 ggcgcgctcg cggccgaggc ggccagcgtc gttgcctgaa gccgcgcccc ctgacaaggc
      361 cgcaaaacac gccgcagctc gcggccgccg aatattagga aatcacgttg ggccgccggg
      421 atttgatcgc ggggcaaact gacgttgatt cgccgtaagg cattggtgct gaactcttta
      481 cgctccattt atagggcacg tatggacatt agtgttataa gggttagaag agcaccgcgc
      541 gagtttccct tatgttctcc cggcaccttc ggcgggcgat tgctccgccc ttggcggtca
      601 atcgtcgcaa acgaactttt caaagagcgg cacagtgctc ccatatgatt cgatgcgcac
      661 aagggaacat ttattccacg cgttcccgga tttttccgga atttattctc ttgactcagg
      721 tacgcgaccg ccatcaccct ggcgggaagg tggcctgctg ctcgacctgg ccgagcagcc
      781 actgcagcag gctgatcgcc cggcggaggt cgtccgccag gtcgcatagc cgctccagcc
      841 ggcggcgctc cttggggtcc tgctcggcac gggccgccgc gcagatcgcg gcccgcaggt
      901 ccgaggaggt caagtccagc ccttgcagtg ccgaatagct gccggagaca tccgtctcgc
      961 cggccagggc ctccagcagg tcgcgctcga tgatcggcgc tgcttcccgc acctgccgcc
     1021 agttcgcggc tgcctccgcc gacgctgtcc gctgggcctg gaatacgaac gagatgccca
     1081 cgatggcgaa caaggtcagc agcgacagca cgaccatcag gatgacgccg cggcggcggc
     1141 gaaccggcct gggcctgcgc atcggcatgc tctcctcgtg ccggaccccg cgtggcgccc
     1201 ggctattgtc cctagcgctt ggccgcggca acttccgccg cgggcgagcc ggctgcgccc
     1261 agtttggcgg cttcgcgggc ccgcagcagc tggatctcca ggtccagccg cacgtagcgg
     1321 gcgtgcttga acgcctggac cggctcgacc tcgaacttgt acttctcttc gacgatcgcc
     1381 tcgacgctcc gggccgccgc cagcgcccgg tcgagctcgt tcacgcgctc gaccggagtc
     1441 gcggccaaat cgaggctggc cttcgtaaag cgctccaggc tggcgcaaat ggcctccaag
     1501 ctcacgcggc cggcgccata cagcgccatc cgcgcttcca agtcctcccc cgccgcgttg
     1561 tagcgctgct tgagcagccg ctgcagctcg ccgtctccgt tgagaatttc cagcgtcgta
     1621 aaggtgaacg gccgcggctc ggctttcggc gctgctgcct gctgcgcggc tgcaaccccc
     1681 accagaaata agaacgcgcc gacgaaaacg gtgacgctgc ggagcatggc ggactccttg
     1741 ggagaaggga gcgaggaacc cagcactgaa cctatcccgc cacggccggg gaagcaattg
     1801 ctggctatta gccggaagca acctggtccc gtgcccgcgg ctgggcgctg tctcgcggtt
     1861 caccgggagc aagtttgctc ccggctattg cttgtccccc gccggccgcg gcgcgataat
     1921 cgcaccagcg cgcgaggtgc cgcatgtcgc aatccccttc gccggaagtc cgccctggga
     1981 acattctcct gcccccgctg gccggcctgg ggttgggcct ggcggcggcg ctgctcgcct
     2041 gggtgctgat ccagcgcgtg catccggtct ttctgatgcc cgagaacctg cacctgtcca
     2101 tgggcgctcc cgcggcgcag gtcaacgcct atcgggccaa gcagcgcgag ctcgaccgcc
     2161 atcacgcgat gctctacctg ggcagccttg gcgcatttct cggcgcgctg ctaggagttg
     2221 ccgaaggacg cttgcggcga tccatcctgc cggtagcgac cacgcttccc ttaggcgctc
     2281 tgggcggcgt cgccggcggc ctgctggcct gcctcgtgca agagcaggtg cgggtcaacg
     2341 tcgggctggc cgagatcaag cacaccgtca tggcgcagct cgctctctgc ctgccgctgg
     2401 ccggctgcat cggcctgggc ctgggcctgg cgacgcggtc acttcgcggc ctggtgctgg
     2461 cggtgctggc cggcctggcc gcgggagtcc tcgcgggcgt gctctatccc ctgatcactt
     2521 cgacgttctt accggggatc agcaccgatt cgctgctgcc cgaagaggcc agcagccgcc
     2581 tgatgtggct gggcctgctg gcgggcgtca tcggcctggt cgtgccgatc gccgggcggc
     2641 gtcgcaaatc ggcgaccagc aacgcgtagc gggagcatct gcctcaaagc cgcgaccagt
     2701 ggtcgcggtc tacgcaatgc accgtgacca ctggtcgcgg cttaggtgcg gctcactcgc
     2761 cgctgatgat gtcaccgccg tgggcggtgc ccagggcccg ccacgagtcg agcgcgacgt
     2821 tgttcgaata gaaccgcacg gagccgtcgc agagcgcggc catgacgccc gtcggatgcc
     2881 ggctgcgggc cccaatgctc tcttcgttgt tgggatcggg attggccggc ttgggattgg
     2941 gatggttgcg gcagggcgga ttcaagcgat tggagggcgt gcaggatt
//
LOCUS       DASRJB010000010         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3669612, whole genome shotgun sequence.
ACCESSION   DASRJB010000010 DASRJB010000000
VERSION     DASRJB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669612"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..693
                     /locus_tag="VFB80_00290"
     CDS             7..693
                     /locus_tag="VFB80_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZN32213.1"
                     /translation="MIPGLGIAERSREGRILVGRSGELAELEAGQAALEVSLEGQPLG
                     RIVLADSVSETSRAAISELKQLGLSVRMLSGDRRETAEAIARDVGIDEVVAEVKPDEK
                     LAAIARWQNSGQVVAMVGDGINDAPALAAADLGIAIGRGADVALESADVVLSRHDLQL
                     IPKTIRLSRSTLAIIRQNLVWALIYNVLLIPLAAAGILPPILAAAAMALSSVSVVANS
                     LRLRWVRVEI"
     gene            732..1553
                     /locus_tag="VFB80_00295"
     CDS             732..1553
                     /locus_tag="VFB80_00295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN32214.1"
                     /translation="MRQLTVLIPCKDEVQHIRACIESARPIADEILVADSGSTDGTLE
                     VVESLDGCRIIKREYVNSADFKNWAIPQARHPWVLVVDADERVTPQLAAEIRQVLADG
                     PSCNGYSLRRDDYFLGHKIDHCGWSRSRLIRLFRRDVARYKVRRVHADVVVSTGNVGQ
                     LDGRLEHYTCTDLDHFIARQHRYSTWSALDAYDAGKRATWARLLTHAPLRFLQTYFLK
                     GGFRDGRAGLVVCGLIAFYSFLKDAKLWALAHNRVPDEATATAAKAALAAHKQAA"
     gene            complement(1792..>3119)
                     /gene="ftsH"
                     /locus_tag="VFB80_00300"
     CDS             complement(1792..>3119)
                     /gene="ftsH"
                     /locus_tag="VFB80_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZN32215.1"
                     /translation="VGPPGTGKTLLAKAIAGEAGVPFFSLSGSDFVEMFVGVGAARVR
                     DMFQQAEAKAPCIIFIDELDALGKSRGTSIVGGHDEREQTLNALLVEMDGFGSNTGVI
                     VMAATNRPETLDPALLRPGRFDRHVLVDRPDIRGREAILKVHVKNVKLDPVVDLKEVA
                     AITPGFVGADLANLVNEAALLAARGEKNAVGMKEFNEGVERVTAGLEKKQRIMNEDEK
                     QRVAYHESGHALVAYSLPNTDPVHKVSIIPRGLAALGYTMQRPEGDRFLLTQSELESR
                     IQVLLAGTIAEEITYTDISTGAQNDLERATDIARKMVMEFGMSRLGRVNYRESNRSPF
                     LAITGGEESVRSCSETTMREIDQEIRRIIDEAIEKVRHILELRRPSLEALTKMLMEVE
                     SIDAADLKRIIEENSPGPLVVPGTEAPLRLAPPEPVSTEAAAAGMEKSG"
BASE COUNT          510 a          976 c         1035 g          598 t
ORIGIN      
        1 gtcgaggtga ttccgggact gggaattgcc gagcggagcc gcgagggccg gattctggtt
       61 ggccggtcgg gcgaattggc tgagttggaa gcgggtcaag ccgcgctcga agtgtctctg
      121 gagggtcaac cgctcggccg catcgtgctt gccgacagcg tgtccgaaac gagccgggcc
      181 gccattagcg aattgaagca gctcggcctg agcgtgagga tgctctcggg cgacagacgc
      241 gaaacagccg aagcgattgc ccgcgatgtt ggcatcgacg aagtcgtcgc cgaagtgaag
      301 cccgatgaaa agttggctgc gattgcgcgc tggcagaatt ccgggcaagt cgtcgcgatg
      361 gtcggcgatg gtatcaacga cgcgccggcg ctcgcggccg ccgatttggg tatcgccatc
      421 ggccgcggag ccgatgtggc gctcgaatcc gctgacgttg ttctatcgcg gcacgatctg
      481 cagctgatcc cgaaaaccat tcgcctgtcg cgttccacgc tggcaattat ccggcagaat
      541 ctggtgtggg cactgattta taacgtgctg ctcattccgc tcgccgcggc aggcattctg
      601 ccgccgattc tcgccgccgc tgcgatggca ctcagcagcg tcagcgtcgt cgccaacagc
      661 ctgcgactgc ggtgggtgcg ggttgagatt tgatattgac cgctggcggc cggcggaata
      721 ccaatcgcag gatgcggcag ctcaccgtcc tgatcccgtg caaggatgag gtccagcata
      781 ttcgggcctg catcgagtcg gcgcggccga tcgccgatga gattctcgtg gccgattccg
      841 gttcgaccga cggcacgctg gaagtggtcg aaagcctcga cggttgccga attatcaagc
      901 gggagtacgt caactcggcg gacttcaaaa actgggcgat cccgcaggcc aggcatccct
      961 gggtgctggt cgtcgatgcc gacgagcggg tcacgccgca attggccgcc gagattcgcc
     1021 aggtgctcgc cgacgggccg agctgcaacg gctattcgct gcggcgcgac gactacttct
     1081 tgggtcacaa gatcgatcac tgcggctgga gccgctcgcg gttgatccgg ctgttccgcc
     1141 gcgacgtcgc gcgctacaag gtccgccgcg tccacgcgga cgtggtggtt tcgaccggca
     1201 acgtcgggca gctcgatggg cggctggagc actacacttg caccgacctg gatcacttca
     1261 tcgcccggca gcaccgctat tccacctggt cggcactgga cgcttacgac gcgggcaagc
     1321 gggcgacgtg ggccaggttg ctgacgcacg cgccgctgcg gttcctgcag acgtattttc
     1381 tcaagggcgg atttcgcgac gggcgggccg gcctggtcgt gtgcgggctg atcgcgttct
     1441 attcgttcct gaaagacgcc aaactgtggg ccctggcaca caaccgtgtg cccgacgagg
     1501 cgaccgccac ggcggccaaa gcggccctgg cggcgcacaa gcaggcggcg tgacgcgccg
     1561 ctagctaacc gtcatccttc gcccgtcaag cacgttgaca gtcctcgttt gggggcacgc
     1621 cacctgcttg cagctgggat tacacccaat acggcggctt cgtttattcc cagctttgcg
     1681 ccgggcgtag tgtcagcggg agatacatcc ccctggtgct tggctgcgtc gtgtcaggca
     1741 ggcgcaatgc aatcgaattg catcttacca acttgcgccg caagtatcgg gctacccgct
     1801 cttctccata cccgcggcgg ctgcttcggt agagaccggt tcgggcggag ccaggcggag
     1861 cggggcttcg gtgccgggga cgacgagggg gccgggggag ttttcttcaa tgatccgctt
     1921 gaggtccgcg gcgtcgatcg attcgacctc cattagcatt ttggtgaggg cttcgagcga
     1981 ggggcggcgg agctcgagga tgtgccggac tttctcaatg gcctcgtcga taatccggcg
     2041 aatttcctgg tcgatttcgc gcatcgtcgt ttcactgcac gagcgaacgc tctcttcgcc
     2101 cccggtgatc gccaaaaacg gcgagcgatt gctctcgcga tagttcacgc gaccgaggcg
     2161 gctcatgccg aactccatca ccatcttgcg ggcgatgtcg gtggcccgct ccaggtcgtt
     2221 ctgagcgccg gtcgaaatgt cggtgtaggt gatctcctcg gcaatcgtgc cggccaggag
     2281 cacctggatg cggctttcca gctccgactg ggtcaacaag aagcggtcgc cttcggggcg
     2341 ctgcatcgtg tagcccagcg ccgccaggcc gcggggaatg atcgagactt tgtgcaccgg
     2401 gtcggtgttc ggcagactgt aggcgacgag ggcgtggccg ctttcgtgat aggccacgcg
     2461 ctgcttctca tcctcgttca tgatccgctg cttcttttcg aggcccgcgg tcacgcgctc
     2521 gacgccttcg ttgaactcct tcatgccgac agcgttcttc tcgccgcggg cggccagcaa
     2581 ggccgcttcg ttcacgagat tcgccaggtc cgcgccgacg aagccgggcg tgatcgccgc
     2641 cacttccttg agatcgacaa cgggatcgag cttgacgttc ttgacgtgga ccttgaggat
     2701 cgcctcgcgg ccgcggatgt cgggccgatc gacgagcacg tgccggtcaa agcggcccgg
     2761 ccgcagcagc gccggatcga gcgtttccgg ccggttcgtc gccgccatca cgatcacgcc
     2821 ggtgttgctg ccaaagccgt ccatctcgac cagcagggcg ttgagcgttt gctcccgctc
     2881 gtcgtggccg ccgacgatgc tcgtgccgcg gctcttgccc agcgcgtcga gctcgtcgat
     2941 gaagatgatg cagggggcct tggcttcggc ctgctggaac atgtcgcgga cgcgggccgc
     3001 gccgacgccc acaaacatct cgacgaagtc ggagcccgag aggctgaaaa acggcacacc
     3061 cgcctcgccg gcaatcgcct tggcgagcag agtcttgccc gtacccggtg gaccgacca
//
LOCUS       DASRJB010000011         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125 k141_2084,
            whole genome shotgun sequence.
ACCESSION   DASRJB010000011 DASRJB010000000
VERSION     DASRJB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFB80_00305"
     CDS             complement(<1..450)
                     /locus_tag="VFB80_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN32216.1"
                     /translation="MSHPRAGDDAHYLHGTHPGEQDRLSRLNGLLNELALRELALAGG
                     EKILDVGSGLGQLTRAMARKSGLRGGTVGVERSGEQLAEAQRLAASADEADLALFRQG
                     SAEDLPLRDGEWGTFDVVHTRFLLEHVRDPAAVVRQMVRAARPGGRIV"
     gene            505..3375
                     /locus_tag="VFB80_00310"
     CDS             505..3375
                     /locus_tag="VFB80_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZN32217.1"
                     /translation="MAVLERLQGQSIQREFLDWRKPALAAAADYLAAGCAQPPELDLG
                     RTIVVVPGGRAGRRLRELLVALAEERDLTLTPPQIVTQHDLPERLYQPQRPFADDLTQ
                     QLAWATALRSFSPAELKPYLPRPPAAPDTAGWLAVGEVLRQLHLGLAADGMDCKHVLA
                     GAAAVEGFSEHERWQTLCQLQRAYLDVLDRLQLWDKQTARLVAIQKHEVASDRQIVLL
                     GTVDLNRALRQMLDQIAGQVTALVVAPPDLAERFDAYGCLIPGKWTELELPVADDQIE
                     RVDGPADQAEAVTRWLADLGGRYRADQIAVGMPDERLVPQVERQLEQSAVRVRWAIGR
                     KLPDTGPYRLLHVAAEYAARWRFRDLAALVRHPDAFAWLERRLRKEGVHQPDILLALD
                     NYAAERLPATLDEERLKKEPDCAKLLAIFQAARQLIEPLVCQPLTAGEWSERLRSVLA
                     LVYGGQRLDRDRELDRYLLEALKHLGQALDALAAVPAELQLRLDVREACRLALDSVAG
                     QGLAPPADPDAVELLGWLELPLDDAPALVVTTFNEGYVPSALSADAFLPNRLRQHLGL
                     LDNDRRLARDAYALSVLLASREKLKLLVAHRDTDGNPLTPSRLLFAADTDRVVERARA
                     FFRELPAAPPRRNLLAAGGTRLISALEPPRPPAGIPPLTGLSVTQFRDYIACPYRFYL
                     RHVLKLEALGDWADELDGGAFGNLVHLVLEQFGRADEARELRTSTEPGPIAGFLEEKL
                     DRIAGARYGKQARAAVRVQIAQARLRLRAFAHWQAQRTGEGWRIVFSEDSEQRRTLTA
                     EFAAEGGPFSLRGRIDRIDYHEQRRILAVLDYKTADAGFAPERTHRRAGAWIDLQLPL
                     YRHLLGAAGLPIELKNLAGIELGYVLLPKDSLGVKHAPADWDEVLLSSADALARQVIA
                     AIRAGKFWPPKSPPPDFSEDFAAICQDRRLGSWMPIGEEEAP"
     gene            3372..>5601
                     /locus_tag="VFB80_00315"
     CDS             3372..>5601
                     /locus_tag="VFB80_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN32218.1"
                     /translation="MSGRPKFPHLVIRASAGTGKTHQLATRFIGLLAAGAAPDQILAT
                     TFTRKAAGEILDRVLFRLAEAAADDAVRQKLAQEIGQPLDREACRRLLVDTVRQLNRL
                     HVGTLDSYFIRVATNFGQELGLPPGWAIGDELADAALRDDAIERLLADGQSAELLTLV
                     HALAKGETQRSVGRLVRDTVSGLFEVFREADAGAWDKLRYPAALSPEELDQTIEALAA
                     LEIAGKRINTARGEDVQRALAADWEWFIGRGLATKVLSGENAFYKQPLPPPLIDLYRR
                     LLQHAASLLVGQIARQTKATQDLLQQFAAHYFALQLEQRSLRFSDVTFRLADALALEG
                     HERLAFRMDGGLKHLLLDEFQDTAPPQWRVLAPLARQVTSQTGGSFFCVGDAKQAIYG
                     WRGGEAELFDALSGQLPNLTSSALTSSFRSAPPVIETVNRVFQNLTKHPNLDKLADAV
                     RAWQQAFPEHTTERKTLAGYVTLATAPAAAEGEDQDASVLAYAADQIKATAEAAPRAS
                     IGVLVRTNAAVARLIYLLRDRSIAASEEGGNPLVDSPAVELILSLLRLADHPGDTIAL
                     FHVVHSPLGPPLELSDHRDPAAAARAGQALRRQLLDEGYGVTVYQWARRLAPSCDARD
                     QSRLQQLVELAYDYQPRSTLRSSDFIHLVEQKRIADPTSSGVRVMTIHQAKGLQFDAV
                     FLPELAIQIAGQQESFVAGRPTAAEPFHVVCRLANASVRQFFPRELQQLFDDYTRREV
                     TES"
BASE COUNT          849 a         1922 c         1948 g          882 t
ORIGIN      
        1 cacgatccgc ccgccgggcc gtgccgcccg caccatttgg cgaactacgg ccgccggatc
       61 gcggacgtgc tccagcaaga accgcgtgtg gaccacgtcg aaggtgcccc actcgccgtc
      121 gcggaggggc aggtcctccg ccgagccttg ccgaaatagg gcgaggtccg cttcgtccgc
      181 actcgccgcc aaccgctgcg cctccgccag ctgctcgccg ctccgctcga cgccgaccgt
      241 gccgcctcgc agcccagatt tccgcgccat ggcgcgcgtc agctgcccca gccccgagcc
      301 gacatcgaga atcttctcgc cgccggcgag cgctagctcg cgcagcgcca gttcgttaag
      361 gaggccgttc agccgcgaca ggcgatcctg ttcgcccgga tgcgttccat gcagataatg
      421 tgcgtcgtcg cccgcgcggg ggtgggacac gggatgtctc ccttcgcctt gtcagccggc
      481 ggaagcttgc gataggtttt cggaatggcg gttttagagc ggctccaggg ccagtccatt
      541 caacgcgagt ttctcgactg gcgaaagccg gcgctggctg cagccgcgga ttatctggca
      601 gcaggctgtg cgcagccccc ggagttggac ctgggccgga cgatcgtcgt cgtgccgggc
      661 ggacgggcag gtcggcggct gcgggagctg ctcgtcgccc tggccgaaga gcgggacctg
      721 acgctgacgc cgccgcagat cgtcactcag cacgacctgc ccgagcgact ctatcagccg
      781 cagcggccat ttgccgacga cctcacgcag caactggcct gggcgaccgc cctgcgatcc
      841 ttttcgcccg ccgagctaaa accttacttg ccgcgtccgc cggcggcgcc agacacggcg
      901 gggtggctcg cggtcggcga agtgctgcgg caattgcatt tggggctcgc ggccgacggg
      961 atggactgca agcacgtgct cgcaggggcc gcggccgtgg agggattttc ggagcacgag
     1021 cgctggcaga cgctgtgcca gcttcagcgg gcctatctcg atgtgctcga ccggctccag
     1081 ctgtgggaca agcaaacggc gcggctcgtg gcaatccaga agcacgaagt cgccagcgat
     1141 cggcaaatcg tcctgctggg cacagtcgat ctcaaccgcg cgctaaggca aatgctcgac
     1201 cagatcgccg gccaggtcac cgcgctcgtc gtcgcgccgc ctgatcttgc cgagcggttc
     1261 gatgcgtacg gctgcctgat tcccggcaag tggaccgagc tggagctgcc agtggccgat
     1321 gaccagattg agcgggtgga tggaccggcg gaccaggccg aggccgtcac gcgttggctg
     1381 gcggacctcg gcgggcgcta tcgggccgat cagatcgcgg tcggcatgcc cgacgagcgg
     1441 ctcgtgcccc aggtcgagcg gcagctggag caaagcgccg tcagagtacg gtgggcgatc
     1501 ggccggaagt tgcccgatac ggggccgtac cgcctgctgc atgtcgccgc cgagtatgcg
     1561 gcccgctggc ggtttcgcga cctggcggcg ctggtccggc atccggacgc gttcgcctgg
     1621 ctcgagcgcc gcctgcggaa ggagggagtg caccaacccg acatcctcct cgcgctcgac
     1681 aactacgccg ccgagcgctt gccggcgacg ctcgacgagg agcggctgaa gaaggagccc
     1741 gactgcgcga agctgctggc gattttccag gcggcccgcc agctgatcga gccgctggtc
     1801 tgccagccgc tgaccgcggg cgagtggtcc gagcggctgc ggagcgtact cgccctcgtg
     1861 tacggcgggc agcggctcga ccgcgatcgc gagctcgacc gctacctgct cgaagcattg
     1921 aagcatctgg ggcaggcgct cgacgccctg gccgcggtgc ccgcagagtt gcagctgcgg
     1981 ctcgacgtgc gggaggcctg ccgcctggcg ctggactcgg tcgccggcca gggcctggcc
     2041 ccgccggccg atcccgacgc ggtggagctg ctgggctggc tcgaattgcc gctggacgac
     2101 gcgccggcgc tcgtcgtgac gacgttcaac gagggctacg tcccctcggc gctttcggcc
     2161 gacgccttcc tgcccaatcg cctgcggcag catctgggcc tgctcgacaa cgaccgccgg
     2221 ctggcccgcg atgcttatgc cctgtcggtg ctgctggctt cgcgcgagaa gctcaagctg
     2281 ctcgtcgccc accgcgacac cgacggcaat ccgctcacgc ctagccggct attgttcgcg
     2341 gccgatacgg atcgcgtggt ggagcgggcg cgggcgttct ttcgcgagct gccggccgcg
     2401 ccgccacggc ggaacctgct cgccgcgggc gggacgcggc tcatctcggc gctagagccg
     2461 ccgcggccgc ccgcgggaat tccgccgctg acgggactga gcgtcactca gttccgcgac
     2521 tacatcgcct gcccctaccg gttctatctg cggcacgtgc tcaagctgga agcactcggc
     2581 gactgggccg atgagctgga cggcggggcc ttcggcaacc tggtgcacct ggtgctggag
     2641 caattcggtc gggccgacga ggcccgcgag ctgcggacct cgaccgagcc gggcccgatt
     2701 gccggctttc tggaagaaaa actcgaccgg attgccggcg ctcggtatgg caagcaggct
     2761 cgcgcggccg tccgcgtgca gattgcccag gcccgcctgc gcttgcgggc cttcgcccat
     2821 tggcaggccc agcgcaccgg cgagggctgg cggatcgtgt tcagcgaaga ttcggaacag
     2881 cggcgcaccc tgacggccga gttcgccgcc gaaggcggac cgttttcgct ccgcggccgc
     2941 atcgaccgca tcgattacca cgagcagcgg cgcatcctgg ccgtactgga ctacaagacg
     3001 gccgacgccg gcttcgcgcc cgagcggacg catcgccgcg cgggtgcgtg gatcgatttg
     3061 cagctgccgc tctatcggca tttgctcggg gcggcagggt tgcccatcga attgaagaac
     3121 ctggcgggaa tcgaattggg ctacgttttg ctgccgaagg attcgctcgg cgtcaagcac
     3181 gcgccggccg actgggacga ggttctgctg agttcggccg acgccttggc tcggcaggtc
     3241 atcgcggcca tccgcgcggg aaagttctgg ccgccgaaat cgccgcctcc cgatttctcc
     3301 gaggattttg ccgcgatctg ccaggaccgc cggctcggct cgtggatgcc cattggcgaa
     3361 gaggaagctc catgagcggc cggccgaagt ttccgcacct cgtcatccgc gccagcgccg
     3421 gcacgggcaa gacgcaccag ctggcgacgc ggttcatcgg cctgctcgcg gccggcgctg
     3481 cccccgacca gatcctggcg acgaccttca cccgcaaggc cgccggcgaa atcctcgacc
     3541 gcgtgctgtt tcgcctcgcc gaagcggcag ccgacgacgc agtccggcaa aaactggccc
     3601 aggagatcgg ccagccgctg gatcgcgagg cgtgccgccg gctgctggtc gatacggtgc
     3661 ggcagctgaa ccggctgcac gtaggcacgc tggacagcta cttcattcgc gtggccacga
     3721 actttggcca ggaactgggc ctgccgccgg gctgggccat cggcgacgag ctggcagacg
     3781 cggcgctccg cgacgacgcg atcgagcggc tgctggccga cggtcagagc gccgagctgc
     3841 tgacgctcgt gcatgccctg gccaaaggcg aaacgcagcg gagcgtcggg cggctggtac
     3901 gcgacacggt gagcggcctg ttcgaggtct ttcgcgaagc cgacgccggg gcgtgggaca
     3961 agctgaggta tccggcggcg ctttcgccgg aggagttgga ccaaacgatc gaggccctcg
     4021 cggcgctgga gatcgccggc aagaggatca acaccgcccg cggcgaagat gtgcagcggg
     4081 ccctggctgc cgattgggag tggttcatcg gccggggcct ggcaaccaag gtgctcagcg
     4141 gcgagaacgc gttttacaag cagccgctgc ccccgccctt gatcgacttg taccggcgcc
     4201 tgctccagca cgccgcatcg ctgctggtcg gccagatcgc gcggcagacg aaggccacgc
     4261 aggacttgct ccagcaattc gcggcgcact acttcgctct ccagctggag cagcggtcgc
     4321 tgcggttctc ggacgtcacc ttccggctcg ccgatgcgct cgcgctcgag ggccacgagc
     4381 ggttggcgtt ccgcatggac ggggggctca agcatctgtt gcttgacgag tttcaggaca
     4441 cggctccgcc gcagtggcgc gtgctcgcgc cgctggccag gcaggtgacg agccagaccg
     4501 gcggatcgtt cttctgtgtc ggggatgcca agcaggcgat ctacggctgg cgcggcggcg
     4561 aggcggagct gttcgacgcg ctgagcggcc agctgccgaa tttgacgtcg tcggcgctga
     4621 ccagcagctt ccgttctgca ccgccggtga tcgaaacggt gaaccgggtc tttcagaacc
     4681 tgacgaagca ccccaacctc gacaagctgg ccgacgcggt gcgcgcctgg cagcaggcct
     4741 ttcccgagca cacgacagag cgaaagacgc tcgccggtta tgtgacgctg gccactgcgc
     4801 cggccgccgc tgaaggggaa gaccaggatg ccagtgtgtt ggcgtacgcc gccgaccaga
     4861 tcaaggccac ggccgaggcc gcgccgcggg ccagcatcgg cgtgctcgtg cggacgaatg
     4921 cggccgtcgc tcggctgatc tacctgcttc gcgaccgcag catcgccgcc agcgaagaag
     4981 gtggcaatcc gctcgtcgat tcgcctgccg tcgagctgat cctgtcgctg ctgcgattgg
     5041 ccgatcatcc cggcgatacg atcgcgctgt tccacgtcgt ccattcgccg ctgggcccgc
     5101 cattggagtt gtccgaccat cgagacccgg ccgcggctgc ccgcgccggc caggcgctgc
     5161 ggcggcagct gctcgacgag ggctacggcg tcaccgtcta ccagtgggcg cggcggctgg
     5221 cccccagctg cgacgcccgc gaccagagcc gcctgcaaca gctcgtggag ctggcctacg
     5281 actatcagcc gcggagcacg ctgcggtcga gcgatttcat ccacctggtc gagcagaagc
     5341 ggatcgccga tccgacgtcg tcgggagtgc gggtgatgac gatccaccag gcgaaggggc
     5401 tgcaattcga tgcggtcttc ctgccggagc tggccataca gattgccggc cagcaggagt
     5461 cgttcgtcgc cggccggccc accgccgccg agccgttcca cgtcgtgtgc cgcctggcga
     5521 acgccagcgt gcggcagttc tttccgcggg aattgcagca gctattcgat gactacacgc
     5581 ggcgcgaggt gaccgaatcg c
//
LOCUS       DASRJB010000012         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1413346, whole genome shotgun sequence.
ACCESSION   DASRJB010000012 DASRJB010000000
VERSION     DASRJB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413346"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFB80_00320"
     CDS             <1..204
                     /locus_tag="VFB80_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HZN32219.1"
                     /translation="FRGAGPSYPLARTPAQTLTANFGSYHPSVCQFVLADGSVRALKV
                     SINTTVLGYIADVDDGQVIPDID"
     gene            493..1884
                     /locus_tag="VFB80_00325"
     CDS             493..1884
                     /locus_tag="VFB80_00325"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZN32220.1"
                     /translation="MIILETKEWAWTTFGKCELGDQRRTRRLVKLAQQAAARPDGSTP
                     EQTESWGDCKAAYRLFDQEDVTFQEIIRPHCEQTRSSCRAGDVKLLINDTTEVEFGYA
                     RRVAGLGPTGNGFGRGFFLHSALMVDAADGRVEGLAGQLLFYRKPQAKRRGAKNTRRR
                     SADRESVVWGELVDRVGPAPPGVHWVHVDDRGADDFEVFCRIQAQGHSCVIRAARLNR
                     WLTKPDGQRVQLRALLDELPCRQTRTLEVAAKGTKPPRTATLQLRLAEVRMPLPTVWT
                     PWLREHPPAQPLRLWVVELRETQPPAGVTPLRWVLYTFEEVTTLADAERVIQWYQRRP
                     TIEDYHKALKTGCHVERRYYETAERLERVTGLLSVLAVRLLQLKTAARETPDRPAAEV
                     APTQWIELVQTARKKPVNRQLTIRQFLRAVAGLGGHLGRKCDGEPGWITLWRGFEKLT
                     LLARGADARQRCG"
     gene            2025..>2272
                     /locus_tag="VFB80_00330"
     CDS             2025..>2272
                     /locus_tag="VFB80_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN32221.1"
                     /translation="MVNTLFLPELREMLAANNAFELKEFCTALHPARTAEFMEGLDAA
                     ETWRVLRHAELKLREDIFGYFPHDKQVAIIEGEDRAEVA"
BASE COUNT          399 a          713 c          774 g          386 t
ORIGIN      
        1 ttccgcggcg cgggcccgag ctatccgctg gctcgcacgc ccgcgcaaac cctgaccgcc
       61 aacttcggca gctaccatcc cagcgtctgt cagttcgtgc tcgccgacgg ctccgtccgg
      121 gcgctcaagg tctcgatcaa caccaccgtc ctcggctaca tcgccgacgt ggacgacggg
      181 caggtgattc cggatatcga ctaagtcggt aagggcgggc ttccttgccg cccaagacgg
      241 gctgggaagc ctgagtattt cccagatctt cgtagtttct acagcgttag ccggatgtgc
      301 tagccgcggt tttcgcggtg aatatgcggt tcgcgcggtt ggccggggat ttgcttagta
      361 ggtcgtggac gcgatgcggc gagggggatg ttcgctgccg ggctttaggg aagtcggaag
      421 ccgatctggg tcgcggagca agcggcgcga gccaacagcg gtgacgcgct gcgcacggag
      481 gttctgcacg cgatgatcat tttggaaact aaggagtggg cgtggacgac gttcggcaaa
      541 tgtgaattgg gagatcagcg gcggaccagg cggctggtca agctggccca gcaggcagcg
      601 gcgcgacccg atggcagcac gcccgagcaa acggaatcgt ggggagactg caaggccgcc
      661 tatcgcttgt tcgatcaaga ggacgtgacg tttcaggaga tcatccggcc gcattgcgag
      721 cagacgcgat cgagctgccg cgcgggcgac gtgaaactgc tgatcaacga cacgaccgaa
      781 gtggagtttg gttatgcgcg tcgcgtggcc ggactcggcc cgacgggaaa cggctttggc
      841 cgcgggttct tcctgcattc ggcgttgatg gtggacgcgg ccgacggccg ggtcgagggc
      901 ctcgccggac agctcttgtt ttatcggaag ccgcaggcca aacgccgggg tgcgaagaac
      961 acgcggcggc gttctgcgga tcgggagtcg gtggtgtggg gtgagttggt ggatcgggtc
     1021 ggtccggcgc cgcccggcgt ccactgggtg cacgtggacg accgcggcgc cgatgacttc
     1081 gaggtgttct gtcgcattca agcccaaggc cacagttgcg tgattcgggc ggcgcgactc
     1141 aatcgctggc tgaccaaacc ggacggccag cgggtgcagc tccgcgcgct gctcgacgag
     1201 ttgccctgcc gccaaacgcg gacgttggaa gtcgccgcca aaggaaccaa gccgccccgg
     1261 acggccacgc tccagctgcg gctggccgaa gtccgcatgc cgctgcccac cgtctggacg
     1321 ccctggctcc gcgagcatcc gccggcccag ccgctgcggc tgtgggtcgt cgaactgcgg
     1381 gaaacgcagc ccccagcggg cgtgacgccg ctgcggtggg tgttgtacac gttcgaggag
     1441 gtgaccacgc tggcggacgc cgagcgagtg atccagtggt accagcgccg gccgacgatc
     1501 gaggattatc acaaggccct gaagacgggc tgccacgtgg aacggcgtta ctacgaaacg
     1561 gccgagcggc tggagcgtgt caccggcctg ttgtcggtcc ttgccgttcg cctgctgcaa
     1621 ctgaaaacgg ccgcccggga aacccccgat cggccggcgg ccgaagtggc gcccacacaa
     1681 tggatcgagt tggtgcagac cgccagaaaa aaaccggtga atcgccagct cacgattcgt
     1741 caattcctcc gcgccgtcgc cggcctcggc ggtcacctcg gccgcaaatg cgatggcgag
     1801 cccggctgga tcacactctg gcgcggcttc gaaaaactaa cgctccttgc tcgcggcgcc
     1861 gatgcccgac aaagatgtgg gtaatactca ggcttcctag cccgcctaag acgggctggg
     1921 aagcccgtcc tacaagcctt tgacaggccc actgcctcgc cggatactca acgtttccgc
     1981 gctgacgaaa cttctccgct gggcctttcg ccgctcgctg cctcatggtc aacacgctgt
     2041 tcctgcccga actgcgcgag atgctggcgg cgaacaatgc cttcgagctg aaggaattct
     2101 gcaccgcgct gcacccggcc cgcacggccg agttcatgga gggcctggac gccgccgaga
     2161 cctggcgcgt cctgcggcac gccgagctga agctccgcga agacattttc ggctatttcc
     2221 cgcacgacaa gcaggtcgca atcatcgagg gcgaggatcg ggcggaggtc gc
//
LOCUS       DASRJB010000013         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3670805, whole genome shotgun sequence.
ACCESSION   DASRJB010000013 DASRJB010000000
VERSION     DASRJB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670805"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFB80_00335"
     CDS             complement(<1..615)
                     /locus_tag="VFB80_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32222.1"
                     /translation="MLAALSPVDLGVLALYLGALLAAGLYFSGRQKSTSEFLLGSRSL
                     AWLPLGLSLAMVLAAGATFSELPALAYEQGLKCWAIPAALWIVLPVVVFATAPLVRGL
                     SLDSLYQYLEDRFDARVRLEATLIFLAWRWFWLTAMLSVASEALALALGWRWPAWALI
                     VPLGVIATLYTFLGGMRAVAWCGAVHAGLMLSGLAVVIGGVWWNL"
     gene            759..1286
                     /locus_tag="VFB80_00340"
     CDS             759..1286
                     /locus_tag="VFB80_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN32223.1"
                     /translation="MQAGCFLARRNSPVRGRRAGGRGFTLIEVLMVVAAMAIIAGVVV
                     PQVTTVIDDAKNSAMLRDLRELSMAIERYRMDHNGGAPDQLQNGVLVQLTHVTDAEGN
                     VGTGPQFVYGPYTREMPTNALNGLARVFRINAAPPATLTNRAGWIYHPASGQVWAGLY
                     PEIVDAALVQAEGSL"
     gene            complement(1317..>2138)
                     /locus_tag="VFB80_00345"
     CDS             complement(1317..>2138)
                     /locus_tag="VFB80_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32224.1"
                     /translation="LLKMQLQVGSVWLAVDATGKRIEPPAPAEPAAAAESGDAAGGAA
                     PPEEAAPPADAAAPAAPAVPAKFVFVELRVTNGDTVPHSYASWNSLAGTSAILADGNG
                     QPLEFVPPAATPGVTRHNLVELAPGQTVTDVLVFAAPQGPPGKLKLALANTALLGNVK
                     SSGSTHLGLEIPPDYLLNPPETTVGIEAGAAPAPPAAAAPAAEGDAPPPLANAPPAKP
                     RDPSAPPTLDDFTKDLERQELMKKKEGQGGEFEDLKVPPTAPKKPLPGKAGEKAP"
BASE COUNT          350 a          736 c          726 g          326 t
ORIGIN      
        1 cagattccac cacacgccgc cgatcaccac ggccaggcct gagagcatca gtcccgcgtg
       61 aacggcgccg caccaggcga ctgcccgcat tccgcccagg aaggtgtaga gcgtcgcaat
      121 cacgcccagc ggcacgatca gcgcccaagc cggccaacgc cagcccaagg ctagcgccag
      181 tgcttcgctc gccacactga gcatcgccgt cagccagaac caccgccagg cgagaaagat
      241 cagcgtggcc tccagccgca cgcgggcgtc gaagcggtcc tcgagatatt gatagagcga
      301 gtccagcgac aggccgcgaa ccagcggcgc ggtcgcgaag accacgaccg ggagcacgat
      361 ccacagtgcc gccggaatcg cccagcactt cagcccctgc tcgtaagcca gggcgggcaa
      421 ctcgctgaac gttgcacccg cggccagcac catcgccagc gacagcccca gcggcagcca
      481 ggcgagcgac cggcttccga gcaggaactc gctggtggac ttctgccggc cgctgaaata
      541 aaggccggcc gccagcaggg cgcccaggta gagcgccagc acgccgagat cgaccggtga
      601 aagagcggcc agcatcgcag gttcgagggg agcagcgggc agccgaagcg ctaaattcgg
      661 caaactcagc atcctagggt cgattttgcc gcgcgggcag ccgtcggcag cggttgtgac
      721 gtagcatttg ccttggagac caccattagt gtggcgctat gcaagcgggt tgtttcttag
      781 cgcggcgcaa ttccccggtc cgcggccgca gggccggcgg ccgcgggttt acgttaatcg
      841 aggtgctgat ggtcgtggcc gcgatggcga tcatcgcggg cgtcgtggtg ccgcaggtca
      901 cgaccgtgat cgacgacgcc aagaatagcg cgatgctccg cgacctgcgc gagctgtcga
      961 tggcgatcga gcggtatcgg atggaccata acggcggtgc gcccgaccag cttcagaacg
     1021 gcgtcttggt ccagctcacg cacgtgaccg acgccgaggg caacgtgggg accgggccgc
     1081 agttcgtcta tggcccttac acccgcgaga tgcccaccaa cgccctgaat ggcctggcgc
     1141 gcgtttttcg catcaacgcg gcgcccccgg ctacgctgac caatcgcgcc ggctggatct
     1201 atcatccggc ttccggccag gtttgggcgg gactctatcc cgaaatcgtg gacgccgcgc
     1261 tggttcaggc ggaaggcagc ctgtagccgc cgcctggctc cggattcaag cctccgttaa
     1321 ggcgctttct cgcccgcctt gccgggcagc ggcttcttcg gcgcggtcgg cgggactttc
     1381 aggtcctcga actcgccccc ctggccttcc ttcttcttca tcaactcctg acgttcgagg
     1441 tccttggtga aatcatcgag cgtgggaggc gcgctcggat cgcgcggctt cgccggcggt
     1501 gcgttggcca gcggcggagg cgcgtcgcct tcggcggccg gcgcggcggc ggcgggcggt
     1561 gcgggagcag cgccggcctc gattccgacc gtggtctcgg gcgggttgag caaatagtcg
     1621 ggcggaatct ccaggcccag gtgcgtcgaa cccgagctct tgacgttgcc caggagcgcc
     1681 gtgttggcca gcgccagctt gagctttccg ggcgggcctt gcggagcggc aaagaccaaa
     1741 acgtcggtca ccgtctgccc gggcgccagc tccaccagat tgtgccgcgt cacgcccggc
     1801 gtcgcggccg gcggcacaaa ctccagcggc tgcccattgc cgtcggccag gatggcgctc
     1861 gttccagcca gcgaattcca gctggcgtag gagtgcggta cggtgtcgcc gttggtcacc
     1921 cgcagctcga cgaacacgaa cttcgcaggc acggcaggcg ccgcgggtgc tgccgcgtcc
     1981 gcggggggcg cagcttcctc gggcggagca gcgccgccgg ccgcgtctcc gctctcggca
     2041 gccgctgcgg gttcggccgg cgcgggcggc tcgatccgct tgcccgtcgc atcgacggcg
     2101 agccagaccg agcccacttg cagctgcatc ttgagcag
//
LOCUS       DASRJB010000014         8313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3671175, whole genome shotgun sequence.
ACCESSION   DASRJB010000014 DASRJB010000000
VERSION     DASRJB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671175"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFB80_00350"
     CDS             complement(<1..868)
                     /locus_tag="VFB80_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN32225.1"
                     /translation="MRLVLNLCEWQLVRMISAVALSLGAVLFFVVTVGLVLLPADVER
                     LRQPLYLSVIECRVQPQSERTLSLLWYMPRLDTKGWVHQLAWHDLRGERPQVEFAWPA
                     LEPTCTGRGAAPGELFVGCWDGTLWRVDLSQPKELPRRLPARLEMAPHVLSCSADGRW
                     LAALGPQTLEVIDLSTGQTAWMIGGGQVRTVAMHPREPRLVVARHTGEYAELEAATGA
                     NVRILASFEEPGIQLAFSPTGDRLAIVASGGRHHLLDWSTGRSAWPDGWIGPYSRSSI
                     VQFSPSGDRLITG"
     gene            complement(1157..2164)
                     /locus_tag="VFB80_00355"
     CDS             complement(1157..2164)
                     /locus_tag="VFB80_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN32226.1"
                     /translation="MKPTAGLPAAVSWLLLVGIVSAQDEILAPDAKPVLLQERGAGEG
                     PAWHPELGLLTSGGGHIYRRDREGAMSVYRENAGTNGLLFDREGRLVMCEPVRRRVSR
                     LEKDGQLTVLTDNYEGKKYNQPNDLTIDTKGRIYFSDPRYGDRSDMEIVDSAGRKIEG
                     VYRIDPDGAAGDHKVTRIITHEVDRPNGLVATPDDKYLFVADNNNNTVGGARKLYRFD
                     LKSDGTVDFASQKLIHDWKATRGPDGMKLDAAGRLFVAAGLNRQNPPLETQPEPTAGV
                     YVFAPEGKLLSFIPTPRDETTNCAFGGDGLKTLFITAGGSLWSVQVKTPGRPAWPVLA
                     K"
     gene            complement(2317..3696)
                     /locus_tag="VFB80_00360"
     CDS             complement(2317..3696)
                     /locus_tag="VFB80_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32227.1"
                     /translation="MLRIPLGPTARYCDGLSRRSFLQLGIAGMGAVGLGTLAAMREAS
                     AAAGSRKDTSVILLWLDGGPGHMDMYDMKPDAPPEYRGIWRPISTNVPGIEITELFPL
                     QAKVADKFSIVRSLHHDSGDHFTGGHWMLTGRNAGVGGGSQAGKFPFFGSIATKVLGS
                     RQPGMPPNVAVPYAMSIGLRPGYFAGNYLGPQENPFETEGDPNNDKFQVKNLDLANSL
                     TLDRLQERRSLIAHFDHLRRDCDRNGTLAAIDRFDQQAFDLVTGEKARKAFDLSQEDP
                     KTRERYGRHSWGQAALLARRLVEAGTTFVTCHFGGWDHHWNLQAGYESYLPRIDQLVA
                     ALFSDLAERGLDDKTLVMLCGEFSRTPRMNDGGNGGPPGSMGTPGRDHWGNSMFCLLG
                     GGGVKGGRIVGSTNRLGEAPQDRPVKPGDIHHTIFRVLGVDPHLHFLDHSGRPIPAID
                     HGAVIEELF"
     gene            4216..5604
                     /locus_tag="VFB80_00365"
     CDS             4216..5604
                     /locus_tag="VFB80_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN32228.1"
                     /translation="MTALYLSLVALAAPPSEAVLVEFGSQTCPHCQAMEPILARLAKE
                     GCPVQVIDVDRQPDVARQFKIEGVPTFVALAAGRETGRLKGAATYEMLVALYQKAGGK
                     LPQVVAGAGYQPADVRGQQSAASGAGQQSPQQAALAASVKLRVEDASGYGFGTGTIID
                     THDSEALVVTCGHLFRESRGQGKIMGEVVVAGAVQKVEGQMIGYDLDRDVALVSLRVP
                     GAVAPVAVAPPGHAVRPADAAFTVGCDKGADPTVRQTHITAVNKYQGRPNFTAAGQPV
                     DGRSGGGLFSADGKLIGICNAADPADDEGLYAGLASIHWQLDQVGLSEVYQRSGRAAV
                     AAAQPAQPRDSAVALASFEQPAREAAPLPQLPANPPGAAATEPSPVFNASDDSELVVI
                     VRSKRDPQQRSDVYLVDQAPPDLLARIAQAARTTAEQRAELARARTATAPPRQLPYQQ
                     PGSPPVVRGQHD"
     gene            complement(5671..7341)
                     /locus_tag="VFB80_00370"
     CDS             complement(5671..7341)
                     /locus_tag="VFB80_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32229.1"
                     /translation="MEEIKRQVGRAQRRLVFQQFLTVIGWSLFASLLVAVIGMAIPRL
                     WVLNVQQQTWDWSWMGGGLAAGLLMASIWTWLIRRSRLDAAIEIDRRFGLKERVSSTL
                     SLSSDELTTDIGQALMTDAVRRVERIDVREQFKVSPTWRFLLPVLPVIALIGLSLLPN
                     ATMKKAEASNEQTAEIKKQIKVATVKLQEKIRDSQKKAEELGLKDTDILKEINKELDK
                     LANKETADRKEALLKINDLAKEIEKRKQELGGAEKLKQQLDKLKDVEKGPADKMAEAM
                     KEGDFGKAKEEMQKLKEDLKAGKLSKEDQEKLANQMEQLKERIQQAMQDKKEAEQKLQ
                     EKIEQKLAEGKVGEAAKLQQQLDQMQQNAAQQEQMMQQLAEKLGQAAQAMKEGDANQA
                     MEQLDKLAKEMEGMQEQLDQIENLDELLDQLADAKEAMKCQNCQGQGCAECEGNKDGD
                     GKGKGKNKGKNGGKGLGEGQGEGDRPEEQTDKKFYDTRVAADPKPGESVRIGNAGGPN
                     KAGKTLEAVKQEIQAALAKEPDALEDVTLPRDQRENAKQYFDKIRKGE"
     gene            7639..8091
                     /locus_tag="VFB80_00375"
     CDS             7639..8091
                     /locus_tag="VFB80_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZN32230.1"
                     /translation="MATAVKSEGIHLPTPAENPAADIVIFDGHCKFCTGQVQNLAKWD
                     RGGKRLAFLSLHDPEVARRFPDLSYDQLMEEMYLVDQRGNRYAGAAAFRYLTTKLPRL
                     YALAPLMHIPFTMPLWRWGYKQIARRRYRIQGKTAEACEDGACRVHLK"
BASE COUNT         1326 a         2777 c         2640 g         1570 t
ORIGIN      
        1 cgcccgtgat cagccggtcg cccgacggcg aaaactgcac gatcgacgag cgggagtagg
       61 gcccgatcca gccgtccggc caggcggagc ggccggtgct ccaatcgagc aggtgatgcc
      121 tgccgccgga ggcgacgatc gccagccggt cgcccgtcgg gctgaacgcc agctgaattc
      181 ccggctcctc gaagctcgcc aggatgcgca cgttggctcc cgtggccgcc tcgagctcgg
      241 catactcgcc agtgtgcctg gccaccacca gccgcggctc gcgcggatgc atggcgaccg
      301 tgcggacttg cccgccgccg atcatccagg cggtctgccc cgtggaaaga tcgatgacct
      361 ccagagtctg cggtcccagg gcagccagcc agcggccgtc ggccgagcac gacagcacgt
      421 gcggggccat ttccagccgc gccggcagcc ggcggggcaa ctccttcggc tgcgacaaat
      481 ctacccgcca cagcgttcca tcccagcagc ctacgaacaa ctcgccgggc gcggcgcccc
      541 gcccggtgca cgtgggttcc agcgccggcc aggcgaattc aacctgcggc cgctcgccgc
      601 gcaaatcgtg ccaggcgagc tggtgcaccc agcccttcgt atccagccgc ggcatatacc
      661 aaaggagcga cagagttcgc tcggactgcg gctgcacccg gcactcgatc accgacaaat
      721 acaacggctg ccgcagccgc tccacatcgg ctggcagcag caccagcccg accgtaacga
      781 cgaagaacaa gacggcgccc agcgacagcg cgacggcgct gatcatgcgg acgagctgcc
      841 attcgcacag gttcaggacc agccgcatga cgcatttctc ctggcgggaa cgctactgac
      901 ttgcctcttt ggggccgatt atagacattc tgtcttaaat gtcaaggcgc agcaatttgc
      961 ctggatcctc gtagtggatt cgccggaatt gagacggcct tgggcggcgg ttggattctg
     1021 gcggcggcca ggattttcag gctgcttaga aactattcgt cagttgccgc ggggcattgg
     1081 tttcttgcag gcggtgtcag ttgtcagtgg tcagtcggaa gtcgtttgtt acaaacaact
     1141 gaccactgac aactggctac ttcgctagca caggccacgc cggccgcccg ggcgttttca
     1201 cctgcacgct ccacaggctg ccgccggcag tgatgaagag cgtcttcagg ccgtcgccac
     1261 cgaaggcgca gttcgtcgtc tcgtcgcggg gagtcggaat gaacgacagc agcttgcctt
     1321 ccggcgcaaa gacataaaca ccggccgtcg gttcgggttg cgtttccagc ggcgggttct
     1381 gacggttcag ccccgcggcc acgaacagcc ggccggccgc gtcgagcttc attccgtcgg
     1441 ggccgcgggt cgccttccaa tcgtggatca gcttctgcga ggcgaaatcg accgtgccgt
     1501 cggacttcag gtcgaagcgg tacagcttgc gagcgccgcc gaccgtgttg ttgttgttgt
     1561 cggccacgaa caggtacttg tcgtccggcg tcgcgaccag tccgttgggc cgatcgactt
     1621 cgtgcgtgat gatccgcgtc actttgtgat ctccggcagc cccatcggga tcgatgcgat
     1681 agacgccctc gatcttccgg ccggcgctat cgacgatctc catgtcgctg cggtcgccgt
     1741 agcgcggatc ggaaaagtaa atccggccct tggtgtcgat cgtcaggtcg ttgggctggt
     1801 tgtacttctt gccctcgtag ttgtcggtca gcacggtgag ctggccgtcc ttttccaggc
     1861 gcgacacacg ccgccgcacg ggctcgcaca tcaccagccg gccttcgcgg tcgaagagca
     1921 ggccgttggt gccggcgttt tcgcgataga cgctcatcgc gccctcgcgg tcgcggcgat
     1981 agatgtgtcc gccgccgctg gtgagcaggc ccaattccgg atgccaggcc ggcccttcgc
     2041 ccgccccgcg ctcttggagc agcacgggct tcgcatccgg cgcgaggatc tcgtcctgcg
     2101 ccgaaacaat ccccacgagc agcagccagc tgacggcggc cggcaatcca gcagtcggtt
     2161 tcatgagtgg tcctccagag cagctgacgc cgattgtaat cggccggcgc ctgtaggtcc
     2221 cgcctaccgg gcgggacctg gcagcgcgta caaccaagac cctgcggttg cggctggtcg
     2281 ctgcgcccct tgcggcagaa gggggcctac ccatgcttag aacaactctt cgatcaccgc
     2341 gccgtggtcg atggcgggga tcgggcggcc ggaatggtcg aggaagtgca agtgcggatc
     2401 gacgcccagc acgcggaaga tcgtgtggtg aatgtcgccc ggcttgaccg gccggtcctg
     2461 cggggcttcg cccaggcggt tggtcgagcc gacgatccgc ccgcccttca cgccgccgcc
     2521 gccgagcaaa cagaacatcg agttgcccca gtgatcgcgg ccgggagtgc ccatcgagcc
     2581 cggcgggccg ccgttgccgc cgtcgttcat ccgcggcgtg cggctgaact cgccgcagag
     2641 catgacgagc gtcttgtcgt ccagtccgcg ctcggccagg tcgctaaaca gggccgcgac
     2701 gagctggtcg atcctaggca ggtagctctc gtagcccgcc tgcaggttcc agtggtggtc
     2761 ccagccgccg aagtggcagg tgacgaaggt cgtgccggcc tcgacgaggc gacgggccaa
     2821 tagtgcagcc tggccccagc tgtgccggcc gtagcgctcg cgggtcttcg ggtcttcctg
     2881 cgacaggtcg aacgccttgc gggccttttc gccggtcacc aggtcaaacg cctgttgatc
     2941 gaagcggtcg atggccgcca gcgtgccgtt gcgatcgcag tcgcgccgca ggtgatcgaa
     3001 gtgcgcgatc aggctgcgcc gctcttgcag gcggtcgagc gtgagcgagt tcgccaggtc
     3061 gagattcttc acctggaact tgtcgttgtt cggatcgcct tcggtctcga acgggttttc
     3121 ctgcgggccc aggtagttgc cggcaaagta gcccggccgc aggccgatgc tcatggcgta
     3181 cggcacggcg acgttgggcg gcatacccgg ctgccgcgag ccgagcacct tcgtggcgat
     3241 cgacccgaag aacgggaatt tcccggcttg cgagccgccg ccgacgccgg cgttgcggcc
     3301 agtcagcatc cagtggccgc cggtgaagtg gtcgcccgag tcgtggtgca gcgagcggac
     3361 gatcgagaac ttgtcggcga ctttcgcctg gagcggaaac agctcggtaa tctcaatgcc
     3421 gggaacgttg gtcgaaatcg gccgccagat gccgcgatac tccggcggcg cgtcgggctt
     3481 catgtcgtac atgtccatgt gcccggggcc gccgtcgagc cagagcagaa tgaccgacgt
     3541 gtccttgcgc gaacccgccg ccgccgaggc ctcccgcatc gccgccaaag tgccgagtcc
     3601 gaccgcgccc attccggcga tgcccagctg caaaaagctg cgtcgcgaaa ggccgtcgca
     3661 atagcgcgcg gtcggtccta gggggatacg gagcatgaga accccaagcg gatgtggtgc
     3721 agacagtcct gtctgccatg tggcgggatg tacgccgttc ctgcggcgag gtgggagctg
     3781 ccctccggac aagtcgtcct tttcgcgaca acgtgtccga tctgcaactt tggatcggaa
     3841 ttgtaaatcg gtcaaatcca gaaagtcaat cgaaaagcga gttttcggcg cgccgcgtgc
     3901 aactctgttg cggccggcca gaaacgactt ttcgtcgctt ctggcccacg cagcgcaaag
     3961 ccttctgccg tcccgcgtta cggcagaagg gccaggaggc cggtttttgc ggccgcctat
     4021 cttcgcaaag cggaggaaag attgctcttg taacaatttc aactccgggc cagattgatc
     4081 ccgttgtggc gattccgcaa gctcgccacg gctgacccga aaacccgcga ccccgaatga
     4141 atcggggcag agcggcagcc ggcatcccgc ggccttgtgc cacgccggcc gccgccaact
     4201 tccaaggagg gaagcatgac agcgctctat ctttcgctcg tggctctcgc cgccccgccg
     4261 agcgaggccg tcctggtgga gttcggctcg cagacttgcc cgcactgcca ggcgatggag
     4321 ccgatcctgg cccgcctggc caaggaaggc tgcccggtgc aggtgatcga cgtcgatcgc
     4381 cagccggacg tggcccggca attcaagatc gagggcgtgc cgacgttcgt cgctctcgcc
     4441 gccggccgcg agaccggacg cctgaagggt gcggcaacgt acgaaatgct cgtcgccttg
     4501 tatcaaaagg cgggcggcaa gctgcctcaa gtcgtcgccg gcgcgggcta tcagccggcg
     4561 gatgtccgcg ggcagcagtc cgcggcgagc ggcgccgggc agcagtcgcc gcagcaagcg
     4621 gcgctggccg cctcggtgaa gctgcgggtc gaagacgcca gcggctacgg cttcggcacc
     4681 gggacgatca tcgacacgca cgacagcgag gcattggtcg tgacctgcgg gcacttgttc
     4741 cgcgagtccc gcgggcaagg caagatcatg ggcgaagtgg tcgtcgccgg cgcggtgcaa
     4801 aaggttgaag gccagatgat tgggtacgac ctggaccgcg atgtggccct cgtcagcctg
     4861 cgcgtgccag gagcggttgc gccggtcgcc gtcgcgccgc cgggacacgc cgtgcggccc
     4921 gccgacgccg ccttcaccgt cggctgcgac aagggagcgg atcccaccgt aaggcagacg
     4981 catatcactg cggtcaataa gtaccagggt cggccgaact tcaccgcggc cggccagccg
     5041 gtcgatggcc gcagcggcgg cggcctgttc tcagctgacg gcaaactgat cggcatctgc
     5101 aacgccgccg atccggccga cgacgaaggc ctgtacgccg gcctggcgtc gatccactgg
     5161 cagctcgacc aggtcggcct gtcagaggtg tatcagcgca gcggccgcgc ggcggtggct
     5221 gccgcgcagc ccgcgcagcc gcgggattcg gccgtcgctt tggccagctt cgaacagccg
     5281 gcccgcgagg ccgcgccgct gccgcagctg ccggctaatc cgccgggtgc cgccgccacg
     5341 gagccctcgc ccgtgttcaa cgccagcgac gacagcgagc tggtcgtgat cgtccgctcg
     5401 aagcgcgatc cgcagcagcg gagcgacgtc tatctcgtcg atcaagcgcc gccggacctg
     5461 ctggcccgga tcgcccaggc ggcccgcacg acggccgagc agcgggccga gctggcccgg
     5521 gcccgcacgg ccacggctcc gccgcggcag ctgccctatc agcagccggg ctcgccgccg
     5581 gtcgtccgcg ggcagcacga ctagcgacga cgcaagaaac gaaagcgccg cgctcgttgg
     5641 ggagcgcggc gctggtgaat tcgcgcgggt ctattcgccc ttgcggatct tgtcgaagta
     5701 ctgcttggcg ttctcgcgct ggtcgcgcgg cagcgtgacg tcttccaggg cgtcgggctc
     5761 tttggccagg gcggcctgaa tttcctgctt cacggcttcc agcgtcttgc cggccttgtt
     5821 cgggccaccg gcattgccga tgcggacgct ctcgccgggc ttcgggtcgg cggcgacgcg
     5881 ggtgtcgtag aacttcttgt cggtctgctc ttcgggacgg tcgccttcac cctggccctc
     5941 gccaaggcct ttgccgccgt tcttgccctt gttctttccc tttcccttgc cgtcgccgtc
     6001 cttgttgcct tcgcactcgg cacagccctg gccctggcag ttctggcact tcatcgcttc
     6061 cttggcgtcg gccagctggt cgagcagctc atcgaggttc tcgatctgat cgagctgctc
     6121 ctgcatgccc tccatctcct tggcgagctt gtcgagctgc tccatcgcct ggttcgcgtc
     6181 gccttccttc atcgcctgag cggcctggcc gagcttctcg gccagctgct gcatcatctg
     6241 ttcttgctgg gccgcgttct gctgcatctg gtcgagctgc tgctggagct tggcggcctc
     6301 gccgaccttg ccttcagcca gcttctgctc gatcttctct tggagcttct gctcggcttc
     6361 cttcttgtcc tgcatcgcct gctggatccg ctccttgagc tgctccatct ggttggcgag
     6421 cttctcctgg tcctccttgc tcagcttgcc ggccttgagg tcctccttga gcttctgcat
     6481 ctcctccttg gccttgccga aatcgccttc cttcatagcc tccgccatct tgtcggccgg
     6541 gcccttctcg acgtccttca gcttgtcgag ctgctgcttg agcttctcgg cgccgcccag
     6601 ctcctgcttc cgcttttcga tctccttcgc gaggtcgttg atcttcagca gggcctcttt
     6661 gcggtcggcc gtctctttat tggccagctt gtcgagctct ttgttgatct ccttgaggat
     6721 gtccgtgtct ttcaggccca gctcttcggc cttcttctgg ctgtcgcgga ttttctcctg
     6781 cagcttgacc gtcgcgacct tgatctgctt cttgatttcg gccgtctgct cattcgaggc
     6841 ttccgccttc ttcatcgtcg cgttcgggag tagcgacagg ccgatcagtg cgatcaccgg
     6901 cagcaccggc agcaggaacc gccacgtggg cgaaaccttg aactgctcgc ggacgtcgat
     6961 ccgctccacg cggcgaacgg cgtcggtcat cagggcctgg ccgatatccg tggtcagctc
     7021 gtcgctcgac agcgacagcg tgctggagac ccgctccttg agcccgaagc ggcggtcgat
     7081 ctcgatcgcg gcgtccagcc ggctgcggcg gatcagccag gtccagatgc tggccatgag
     7141 caggccggcc gccaggccgc cgcccatcca gctccagtcc cacgtctgct gctgcacgtt
     7201 cagcacccac agccgcggga tcgccatacc gatcaccgcc acgagcagcg aggcgaagag
     7261 cgaccagccg atcacggtga ggaactgctg aaacaccagt cgccgctgag cccgacccac
     7321 ttgccgtttg atttcttcca tggccgatcc ctttgacttg acgctgatta gtcgtgctgg
     7381 tcgctcgctg cggagccgag aattcgtccc cgaaatagga ttcgtcgcca gagccccggc
     7441 attatagacc ttttgtctgg ataaatcgcc agtgccctga aaatttttcc gcgaaagtgc
     7501 atcttcgccc tgacaataga gacggtcggt cggccgcttc cgcttttctc ccgcgactgc
     7561 cagctttacc cgcaggaaaa agccagcgtt tcacggcttt gggttgcccg ccgccggctg
     7621 ccgcggtcca atggatatat ggctaccgcc gtcaaatccg agggaatcca cctgccgacg
     7681 ccggcggaaa acccggccgc cgacattgtc atcttcgacg gccactgcaa gttctgtacg
     7741 gggcaggtgc aaaacctggc gaaatgggat cgcggcggga agcggctggc attcctgtcg
     7801 ctgcacgatc ccgaagtcgc aaggcgtttc ccggatttga gttacgatca gctcatggag
     7861 gaaatgtacc tcgtggatca gcgcggaaac cgctacgccg gcgccgcggc ctttcgctac
     7921 ctaactacca aattgccaag gctttacgcg ctcgccccgc tgatgcacat cccgttcacg
     7981 atgccgctct ggcggtgggg ctacaagcaa attgccaggc ggcgctaccg cattcagggc
     8041 aagactgccg aggcgtgcga ggacggcgcc tgccgggttc acctcaagta agcgaatatt
     8101 gcggcccctg gattggtggg tcggcgcacg cctgaaatca caatttgcat cgcaaacctt
     8161 cgctgactaa tgacttccgt cgagtgctca aaaatcggtt aagtagtact tattagcctt
     8221 agagaacggt ctctcgttcg tgtccgttgc ggcagctcac cagaatggca gccgttcgcg
     8281 ccaaaatgaa actatggtgc aaatagcgta caa
//
LOCUS       DASRJB010000015         4606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2543392, whole genome shotgun sequence.
ACCESSION   DASRJB010000015 DASRJB010000000
VERSION     DASRJB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543392"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(726..1778)
                     /locus_tag="VFB80_00380"
     CDS             complement(726..1778)
                     /locus_tag="VFB80_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZN32231.1"
                     /translation="MPTLAPPVLQTFAAKLLHAGGMSQGESALVAASLVDANLKGYDS
                     HGVMRVPYYVQAIKDGEVVSGGELKILEEGPARIVADANWGIGQVQAQRLIELLAAKA
                     RAGGLALGTMMHCGHIGRLGEYCELTAASGFVSMLMVNSHGGAVRVAPPGGKAPRLST
                     NPLAIGVPHGSAPLVLDFSTSATAEGKVRVKKIAGQPCPEGWLIDSEGRPTTDPSTLY
                     GNPPGSILPMGGAQAYKGFGLGLMIEILTGALSGGNVAKSVPYPKKGNCVFVLLIDPD
                     RFGGADHFQSEVSQLVEYVKSCPRVAGCDEIILPGDPERRLAAQRLSDGITLDVENWN
                     ALVKLGETLGVGSGRG"
     gene            complement(1850..2743)
                     /gene="rsmH"
                     /locus_tag="VFB80_00385"
     CDS             complement(1850..2743)
                     /gene="rsmH"
                     /locus_tag="VFB80_00385"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZN32232.1"
                     /translation="MPGAPIHVPVLAAEVLHWLDPQPGQVFVDGTLGGGGHTRLLAER
                     VAESPASKDRGFVLALDRDPAAIAAAERNLAGMPVQLAQANFRDVGGVLDELQIPAVD
                     GVLLDIGLSSDQLADARRGFSFDSAGPLDLRFNPNEGEPASQLLARLSERELADLIYR
                     FGEERLSRRIARQIVESRRARPIETAAGLAELVRRCVPRSADRLDPATRTFQALRIAV
                     NRELESLEDALRRIPDRLKRGGRLAIISFHSLEDRRVKEAFRGDERLEILTKKPIRPS
                     ADEIHRNPRSASAKLRVARRR"
     gene            complement(3057..3563)
                     /gene="mraZ"
                     /locus_tag="VFB80_00390"
     CDS             complement(3057..3563)
                     /gene="mraZ"
                     /locus_tag="VFB80_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZN32233.1"
                     /translation="MDQILLTGNYERALDDKLRLALPKQLREPLASAGQLVLTPGTDG
                     SLSLFPQQAFAALAEKLAARSPTGQDVRAFSRLLYSQSHSVEIDSQGRIRLPAEMAKL
                     AALEGEVMLLGVGDRVELWNKSRWEAYLADRAPQYDQLAEAALTDAAPAGSAPPIVET
                     PSRPMQPR"
     gene            complement(3753..>4606)
                     /locus_tag="VFB80_00395"
     CDS             complement(3753..>4606)
                     /locus_tag="VFB80_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN32234.1"
                     /translation="QQAELVQTFLFHANVIGSRAAVQVGVPFLTGLRVADARRWRTSL
                     ERWTTRAARRHVCVSQSVADFYRQRGFPSARLIVIPNGIDVAVWRDARPIDLTQLGVP
                     AGRRVFVFVGRLAEQKGLAPFFQELPAAFRELSDHDLLLVGDGPDRVQLAETAQRLGV
                     AERVHFAGWRPDVPQILAAADMLILPSHWEGMPNVILEAMAAARPVVASRAEGVAELL
                     GDGTGEQTVPTRSAVEFRERLTSLLAKRDSLKLMGSRNQERAIQQFSIENSAAAYERL
                     FSAIART"
BASE COUNT          812 a         1483 c         1558 g          753 t
ORIGIN      
        1 gccagcagga tcaagagcgt gcggaggcga tagcgcatgg cgttagttct cggacgactt
       61 cctgtccccc gcgaatccaa caagcacaac gaccacaccc accgcgacta cgagcagcac
      121 tagcccgcat ttctcacagc gcggaaggat gacgtccgaa ttggtggtgg ggcggggtgg
      181 tagccggcca ggggtttggt gcaggtgcgg tggggtggcg gtttctggcg gcggagtgtg
      241 tttcttgctc tgacggcggt cggtgcgcag tcggggagcg cggagatagt cgatggcgcg
      301 tacgggagcg gttagcgagt gaccttccag aaaaatccaa gattgcggga aaccacacgg
      361 tactttctat gtggacgggt gtttaataag aggcggtgcg cgcggccgcc tcgtgctgtt
      421 ctcaagttgg tggatggatg ttcctaaggg cggcgattgc gccgctgtgc aggccagtgg
      481 tggcgggggt aaggataatt cccaagagag ctctctaaca cttcaaaccc aactgaccag
      541 gcggcagtca ggcggcgaca taactgaagc gcggacaagc aattacgatc ggctccgcgg
      601 actcaggcga cttgcaaaaa cctggagcag cgggaattat ccttacccct gccaagcgtc
      661 aaatcggtgg gacgcacgag cggctaaggg cagcggcgta ggaatgtcaa acggggacaa
      721 tccagtcacc cccggcccga gccgacgccg agcgtttcgc ccagcttcac cagagcgttc
      781 cagttctcca cgtcgagcgt gataccatcg ctcaggcgct gggcggcgag ccgccgctcg
      841 ggatcgccgg gcaggatgat ttcgtcgcag ccggcgacgc gggggcagga cttcacgtat
      901 tcgaccagtt gcgagacttc cgactgaaag tgatcggccc cgccgaagcg gtccgggtcg
      961 atgagcagca cgaagacgca gttccctttt ttcggatagg gtacgctctt ggcaacgttg
     1021 ccgccggaga gcgcgccggt gaggatttcg atcatcaggc cgaggccaaa gcccttgtac
     1081 gcttgcgccc cgcccatcgg caggatcgaa ccgggcgggt tgccgtagag cgtgctcggg
     1141 tcggtcgtcg gccgcccttc gctgtcgatc agccagcctt cggggcaggg ttgcccggcg
     1201 atcttcttga cccgcacctt gccttcggcg gtggcgctgg tcgagaagtc gagcacgagc
     1261 ggcgcgctcc cgtgcggcac gccgatcgcc agcgggttgg tcgagagccg cggcgccttg
     1321 ccacccggcg gcgcgacgcg tactgcgccg ccgtgcgaat tgaccatgag catcgaaacg
     1381 aaaccggagg ccgccgtcag ctcgcagtac tcgcccagcc gtccgatgtg gccgcagtgc
     1441 atcatcgtgc cgagggccag tccgcccgcg cgggccttgg cggccagcag ctcgatcagc
     1501 cgctgggcct ggacctggcc gatgccccag ttggcgtcgg cgacgattcg cgcgggccct
     1561 tcctccagga ttttcagctc accgccggaa acgacttcgc cgtccttgat cgcctgcacg
     1621 taatacggca cgcgcatcac gccgtgcgag tcgtagcctt tcagattcgc atcgacgagg
     1681 ctggcggcca cgagggccga ctcgccctgc gacatgccgc cagcgtgcag cagtttggcg
     1741 gcgaaagttt gaagaacggg cggggcgagc gtgggcatgg gaactcgaat caggcgggag
     1801 ggaggcgcga tgcgaatgta cggtgcggcc gcccgaggcg tcaatgacgc taccgccgcc
     1861 gcgcgacgcg cagcttcgcc gaggcgctgc gtggattgcg gtggatttcg tcggcgctgg
     1921 ggcggatcgg ttttttggtg aggatctcca ggcgttcgtc gccgcggaag gcctccttga
     1981 cgcggcgatc ctcgagcgaa tgaaagctga tgatggccag ccggccgccc cgtttcagcc
     2041 gatcgggaat tcgccgcagg gcgtcttcca gcgactccag ctcgcggttg acggcgatcc
     2101 gcagcgcctg gaaggtccgc gtcgccgggt ccagccggtc ggccgagcgc ggcacgcagc
     2161 gccggacgag ctcggccagc cccgcagcgg tctcgatcgg ccgcgcgcgg cgcgattcga
     2221 caatctggcg ggcgatccgc cggctgagcc gctcctcgcc gaaccgatag atcaagtcgg
     2281 ccagctcccg ctcggagagt cgggccagga gctgcgacgc cggctcgcct tcgttgggat
     2341 tgaaccgcag atcgagcggg ccggccgagt cgaagctgaa gccgcgccgc gcgtcggcca
     2401 gttgatcgct ggagagtcca atgtcgagca gcacgccatc tacggccgga atttgcagct
     2461 cgtcgagcac gccgccgacg tcgcggaaat tggcctgcgc gagctgcacc ggcattcctg
     2521 ccagattccg ctcggccgcg gcgatcgcgg cgggatcgcg gtccagcgcc agcacaaagc
     2581 cgcggtcctt cgaggcgggc gattcagcga cccgctcggc cagaagacgc gtgtggccgc
     2641 cgccccccag cgtgccatca acgaaaacct gtcccggctg tggatcgagc cagtgcagca
     2701 cttcagccgc cagaacgggc acgtggatcg gcgcaccagg catccggccg aagatagctc
     2761 actcaccaaa gccgcgacca gtggtcgcgg tcctccattc accggcccgc gttcgcggtc
     2821 aagcgggtgg ggcttcgacc cacaggcgcc tccgtgcgcc tggggctggg tcgaggcccg
     2881 cgggatctgg ccggtgcgat cacggcacga tctcgcgttg gcggccggga gaatcgttct
     2941 cccggaacgg gcgcgcaggg tgctgccggc ggcaggtggc cgctggccgc cggcactctg
     3001 gtcgctcgct gccaaccacg gacggacgta ctcggtcgtg tctcctgttt cgaaaatcac
     3061 cgcggctgca tcggccgcga cggggtctcg acaatcggcg gtgcggagcc ggcgggcgcg
     3121 gcgtcggtca acgcggcttc cgccaactgg tcgtactgcg gcgcgcggtc ggccagatac
     3181 gcctcccacc gcgacttgtt ccacaactcc acgcggtcgc ccacccccag cagcatcact
     3241 tcgccttcca gtgccgccag cttcgccatc tcagccggca gccgaatgcg accctgcgag
     3301 tcaatctcga cgctgtgcga ctgggaataa agcaggcggc tgaacgcccg gacgtcttgc
     3361 cctgtcggcg atcgagccgc cagcttttcg gccaaagccg caaaagcctg ttgcgggaag
     3421 agcgagagcg aaccatccgt gcctggagta aggaccaatt ggccggccga ggcgagcggc
     3481 tcacgaagct gcttcggcaa ggccagccgc agcttgtcat cgagcgcccg ctcgtagtta
     3541 ccggtcagga ggatttggtc catttctccc cactctccac cacatggcac cacaacatac
     3601 gaatcgaaaa tgattcgtca acacggtctg gaggcggttt accgcatcgc gcgagatgct
     3661 ggtggctgaa aggcggccga tgaagcgcgg aattggggag ttcgaaaccg taaggcaaag
     3721 gaacccgccc ctgagaagaa attttgctcg gttcaggtgc gcgcgattgc ggaaaacagt
     3781 cgttcgtagg cagccgcaga attctcaatg gaaaattgtt ggatagcacg ttcttgatta
     3841 cggcttccca tcaacttcag ggagtcccgt ttcgccagca ggctcgtcag tcgctcgcgg
     3901 aactcgacgg cggatcgcgt gggcacggtt tgctccccgg tgccgtcacc gagcagttcg
     3961 gcaactccct cggcgcgaga tgcgacgacg ggcctggccg cggccatcgc ctcgaggatg
     4021 acgttcggca tgccctccca gtgcgagggc aggatcagca tgtcggcagc ggccaagatt
     4081 tgcggcacgt ccggccgcca gccggcgaag tggacccgct cggcaacgcc cagtcgttgc
     4141 gccgtttccg ccagctgcac ccggtcgggc ccatcgccga cgagcagcaa atcatggtca
     4201 gacaattcgc gaaatgcagc cggcaactcc tgaaagaacg gagcgagccc tttttgttcg
     4261 gccagccggc cgacgaagac aaagacgcgc cggccggccg ggacgccgag ctgcgtcaga
     4321 tcgattggcc tggcgtcgcg ccaaacagcc acgtcgatgc cattgggaat cacgatcagc
     4381 ctggcactag gaaagccgcg ctgccggtag aaatcggcga cgctttggct gacgcagacg
     4441 tgccgccggg cagcccgcgt cgtccagcgt tccaggctgg tgcgccagcg ccgcgcgtcg
     4501 gctacgcgca gcccggtgag aaacggcacg ccgacctgca cggcggcgcg ggacccgatc
     4561 acgttggcat gaaacaggaa cgtctgcacg agttcggcct gttgct
//
LOCUS       DASRJB010000016         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3672494, whole genome shotgun sequence.
ACCESSION   DASRJB010000016 DASRJB010000000
VERSION     DASRJB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3672494"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFB80_00400"
     CDS             complement(<1..431)
                     /locus_tag="VFB80_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN32235.1"
                     /translation="MKILLLGTAGYHPNERRHTACVMLPELGIVLDAGTGFFRVREHL
                     QTPTLDILLTHAHLDHVAGLTFLLDTAWERGLSRITVHGEAPKLAAIREHLLSELLFP
                     APLNCEWQPLGERPLAIAGLKVTHFPLEHPGGCVGYRLDWPG"
     gene            complement(448..1050)
                     /locus_tag="VFB80_00405"
     CDS             complement(448..1050)
                     /locus_tag="VFB80_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN32236.1"
                     /translation="MSSVPEPRLSPLEYLARERTAERKSDYFQGRSFAVAGASPNHHR
                     VVRNVLAQLDAQLKGGPCEIFPSDLRLSCPAGLMTYPDAMVICGPLEYRIGTDDTVTN
                     PKLIVEVLSKASEGYDRGQKFAFYREIPTLHEYLLAAWHAPLVERFQRQQGGGWLLTE
                     ARGMDALLRLPVIDCVLPLAEVYRDVVFPAVAGGLLVGDE"
     gene            complement(1128..2585)
                     /locus_tag="VFB80_00410"
     CDS             complement(1128..2585)
                     /locus_tag="VFB80_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN32237.1"
                     /translation="MSKPSAPVDQSPQTLPRILGPFDAVMIVVGSIIGSGIFLKVSTI
                     DNLVPSFGTIMAVWVVGGLATLCGSLSLAELAAMLPHAGGPYVYLREAYGRIAAFLWG
                     WAEFSIIRTGSLGSLACGTVIYLNKFLDSLETNGALPAFLADLVPLSHVAQAAATILA
                     VLTLTWINIIGTRSAARTQNITAVIKVGFLIVLMLGPLALGHWNTANLRPIAPPTIDL
                     NLFKAFGLAMVAVFWPYDGWINIGPVAEEVRQPERNVPLGLGLGVLVVTLVYCGANVG
                     YHLCLPLDKTMKSQAIAADVFETILGPYGVPLAAAGVMISMFGALNSNLMAGPRIYFA
                     MARDRLFPSAIQQIHPRFKTPTNAILAQSAWSVTQIVIAFALTKDPKAAFDDLTDFVV
                     LGGTIFYALVVAAIFVLRVKMPKAERPYRTWGYPITPALYLLTAALVVGSLVLEAFAP
                     DLEFYKRLKVPAVGLLMVIGVALYYFFRRLEQPKS"
     gene            2777..>3684
                     /gene="gcvT"
                     /locus_tag="VFB80_00415"
     CDS             2777..>3684
                     /gene="gcvT"
                     /locus_tag="VFB80_00415"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZN32238.1"
                     /translation="MTGPLHRTPLGDWHSAHGGRMVDFAGWSMPVQYGSIVAEHQATR
                     TAAGLFDVSHMGRLRLDGGGAEAFLDRLLTRKVVGQGPGKIRYSLVCNASGGILDDVL
                     VYHLQESRGGLYHLVVVNASNREKIVAWFRSQLSPADDVQLADRTQETAMIAVQGPAA
                     LRCVEPLVGADIGGLGYYTGTETTICGQRGVVSRTGYTGEDGCELIVPAAAAVEVWEK
                     VLAHGKSTGAMAAGLGARDTLRLEAAMPLYGHELSEQINPLQAGLSFAVNLEGRDFLG
                     RAALVAAKQNKEQPVRVGLELAGRRAA"
BASE COUNT          659 a         1160 c         1299 g          566 t
ORIGIN      
        1 cctggccaat ccagccggta gccgacgcag ccgccgggat gctccagcgg aaagtgcgtg
       61 accttcaggc ccgcgatcgc cagcggccgc tcgcccagcg gctgccactc gcaattcagc
      121 ggcgcgggaa agaggagttc gctgagcagg tgctcgcgga tcgccgccag cttcggcgcc
      181 tcgccgtgca cggtgatccg cgacaggccg cgctcccagg ccgtgtcgag caggaacgtc
      241 agccccgcca cgtggtcgag atgggcgtgc gtcagcagga tgtcgagcgt gggcgtctgc
      301 agatgctcgc ggacgcgaaa gaaccccgtg cccgcgtcga gcacgatccc cagctccggc
      361 agcatcacgc aggcggtgtg tcgccgctca ttgggatggt agccggcggt gcccaggagg
      421 aggattttca tcgctggcca aaatccctca ttcatcgccc acgagcaggc cgccagcgac
      481 tgcaggaaac acgacgtcgc gatagacctc ggccagcggc aggacgcagt cgatcaccgg
      541 cagccgcagc agagcgtcca tccccctggc ctcggtgagc agccagccgc cgccctgttg
      601 ccgctggaat cgctcgacca gcggcgcgtg ccaggcggcg agcaaatact cgtgcagcgt
      661 gggaatctcg cggtagaacg cgaatttctg gccgcggtcg tagccctcgc tcgctttcga
      721 gaggacctcg acgatcagct tggggtttgt caccgtgtcg tcggtgccga tgcgatactc
      781 cagcggcccg cagatgacca tggcgtcggg ataggtcatc aggcccgccg ggcagctcag
      841 ccgcaagtcg ctggggaaga tttcgcaggg cccgcccttg agctgggcgt cgagctgggc
      901 caggacgttg cggacgacgc ggtggtggtt gggcgaagcg cccgcgaccg cgaacgagcg
      961 tccctggaag taatcgcttt tgcgttccgc cgtccgctcg cgggccaggt attccagcgg
     1021 cgacagtcgc ggctcgggca ccgaagacat ggcggcaatc gaagagaagg acgtagcgtc
     1081 cttgagaata gtcgaacggc ggccgaaaag acaggaaccg aggcgaccta gctcttcggc
     1141 tgctccaggc ggcgaaagaa gtaatagaga gccacgccga tgaccatcag caatccgacg
     1201 gcgggcacct tgaggcgttt gtagaactcg aggtccggcg cgaatgcttc gaggaccaga
     1261 ctgccgacga cgagcgccgc ggtcagcagg tagagcgcgg gcgtgatcgg atagccccag
     1321 gtgcgatacg gcctctcggc cttgggcatc ttgacccgca atacaaagat ggccgccacc
     1381 acgagggcgt aaaagatggt cccacccagc accacaaagt cggtgaggtc atcgaaggcc
     1441 gctttgggat ccttcgtgag ggcgaaggcg atcacgattt gcgttaccga ccaggccgac
     1501 tgggccagga tcgcgttcgt gggtgtcttg aaccgcgggt gaatctgttg gatcgcgctg
     1561 ggaaacagcc ggtcgcgggc catggcgaag taaattcgcg gtccggccat caggttggag
     1621 ttgagcgccc cgaacatcga gatcatcacg cccgcagccg ccagcggcac gccgtaaggg
     1681 ccgagaatgg tctcgaacac gtcggcggcg atcgcctgcg acttcatcgt cttgtcgagc
     1741 ggcaagcaga ggtgataacc gacgttcgcg ccgcaataga cgagcgtgac gacgagcacg
     1801 ccgaggccga ggccgagcgg cacgttgcgc tccggctggc ggacctcttc ggcgacgggg
     1861 ccgatgttga tccagccgtc gtagggccag aagacggcga ccatcgccag cccgaacgcc
     1921 ttgaacaggt tgaggtcaat cgtcggcggc gcgatcggcc tcaggttggc cgtgttccag
     1981 tgccccagcg ccagcggccc gagcatcagg acaatcagga agccgacctt gatcaccgcg
     2041 gtgatgttct gggtgcgggc ggccgagcgc gtgccgatga tgttgatcca ggtcagcgtg
     2101 aggacggcca ggatcgtggc ggcggcctgc gcgacgtgcg acagcggcac gaggtcggcg
     2161 agaaatgcgg gcagagcgcc gttcgtctcg agcgaatcca ggaacttgtt gaggtagatg
     2221 accgtgccgc aggccaggct gccgagcgag ccggtgcgaa tgatcgaaaa ctcggcccag
     2281 ccccacagga aggctgcgat gcggccgtac gcttcccgca gatagacgta cggcccgccg
     2341 gcgtgcggca acatcgccgc cagctccgcc agcgagagcg agccgcaaag cgtggccaga
     2401 ccgcccacga cccagacggc catgatcgtg ccgaacgacg gcacgaggtt gtcgatcgtg
     2461 ctgaccttca gaaagatgcc ggagccgatg atcgaaccga cgacgatcat caccgcgtcg
     2521 aagggcccga ggatgcgcgg cagcgtttgg ggcgactgat cgacgggcgc tgaaggcttc
     2581 gacatgaggg ctccgggcgg ggcagaggca ggatagcgaa tcgcagccgg gtacgcgagg
     2641 gaaacgtgag cggcgttagt ttggttcttc ggtccttcag ttcttgagtt cttcggccgc
     2701 ttctccgcca ctggcacctc gccccggcgg cgatacaatg ccggtgttcc ttcgttcctt
     2761 cacaagttac tgcccgatga ccggcccgct ccacaggacg ccgctgggtg actggcactc
     2821 cgcccacggc gggcggatgg tcgatttcgc gggctggtcg atgccggtgc agtacggctc
     2881 gatcgtcgcc gagcaccagg cgacgcggac cgcggcgggc ctgttcgacg tctcgcacat
     2941 gggccgcctg cggctggacg gcggcggcgc ggaggcgttc ctcgaccgcc tgctgacccg
     3001 caaggttgtc ggccagggcc cgggcaagat ccgctattcg ctcgtctgca atgcgagcgg
     3061 cggcattctc gacgacgtgc tcgtctatca cctccaggag agccgcggcg ggctgtatca
     3121 cctggtcgtc gtcaacgcga gcaatcgcga gaagatcgtg gcctggttcc gctcgcagct
     3181 ttcgccggcg gacgacgtgc agctggccga ccgcacgcag gaaacggcga tgatcgccgt
     3241 gcaagggccg gccgccctgc ggtgcgtcga gccgctcgtc ggcgcggata tcggcggcct
     3301 gggctattac accggcacgg aaacgacgat ctgcgggcag cggggcgtcg tcagccgcac
     3361 gggctacacg ggcgaagacg gctgcgagct gatcgtcccg gcagccgcgg cggtggaagt
     3421 ctgggagaag gtgctggccc acggcaaatc gacgggtgcg atggccgctg ggcttggtgc
     3481 tcgcgacacg ctgcggttgg aagccgcgat gccgctgtat ggccacgagc tttccgagca
     3541 gatcaacccg ctgcaggccg ggctctcgtt cgcggtcaac ctcgaaggcc gcgattttct
     3601 aggccgagcg gcactggtcg ccgcgaagca gaacaaggag cagccggtgc gcgtgggact
     3661 ggagctggcg gggcgtcgcg cggc
//
LOCUS       DASRJB010000017         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_569086, whole genome shotgun sequence.
ACCESSION   DASRJB010000017 DASRJB010000000
VERSION     DASRJB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569086"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFB80_00420"
     CDS             <1..1065
                     /locus_tag="VFB80_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32239.1"
                     /translation="GVGEAGSLHLARTDKASEQDFAIAYVCLPDERVLHFIPGFVSAA
                     ELLAELEWAERCYADVAKLPAAEQPLAARRAHLAAIAKADLPEFARRFPSRWQEDSLP
                     EGESTVDLPNVVAAARATFESMLLMRLGPQWRRDAQAGGLAPLAAHGGLAGELAHFVL
                     SEYPLAALGDLAGPAYAASSGQRFFTYPKRRADLAAWWALERRANKPTLLVIGDDDYF
                     TASSYQPSIPPPRSLRSRMSARANLVESPSPPLAPAADAPPFEWKKAAAGLPDLSKFA
                     VQVVTLDELSLLLSDALLPSVSYVRGQPLRYLVFDARGFRIAQLSSDATPARLAAAMR
                     EALPPASAKQTIGVTRNAKP"
     gene            1052..2530
                     /locus_tag="VFB80_00425"
     CDS             1052..2530
                     /locus_tag="VFB80_00425"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZN32240.1"
                     /translation="MRSLNRCRWLCLPIALALVAPVARGDEEPSPVQEGRQLFERKFT
                     ARNAGNLGDGLGPLFNHVSCAACHVQGGLGGGGPVDVNVVLLSAGLRSSRPEKKQLLA
                     ALREQHPNFVSRSDDISATIILHRFSNDPAYEVFRSKFVLEPVPLDPTAGERRSLQQS
                     LARTAEAEVKTPPPLRLVTTQRNTTALFGAGLIDSIPDGMLRTLAQEQQSEVSGRVPP
                     IGFDRVGRFGWRGQIETLHDFVLGACANELGLEVPGHEQPRDPQRPKYRPVGLDLSAS
                     ECRSLTAFVAQLPAPRMVLPADPDKKALAEQGRDLFGKIGCAACHVERIGPVQGIYSD
                     LLLHDLGEALTDPAPAEATFVATGKRLPPTDESILGTTDGEALKSSIQPQPPRPRGYN
                     GGSQSLVAGPSVVLVPDKETRERIEFRAQPTPVESEWRTPPLWGVADSAPYLHDGRAA
                     SLVEAIALHGGEAEPCTKRFFAQSLPERLAVLEFLGCLRAPQ"
     gene            2635..2862
                     /locus_tag="VFB80_00430"
     CDS             2635..2862
                     /locus_tag="VFB80_00430"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN32241.1"
                     /translation="MTTATLNGSVSQIGEAAGAVWRTLSEHGPQSLAKIVERVGGNRD
                     LVMQGIGWLAREGKLEIAETRRGRIISLLPE"
     gene            3029..>3455
                     /locus_tag="VFB80_00435"
     CDS             3029..>3455
                     /locus_tag="VFB80_00435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911865.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZN32242.1"
                     /translation="MRVLVVGSGGREHALAWKIAASPRVERVFVAPGNAGTAAEAENV
                     PIAADDLPGLVKFVRDAAIDLTVVGPEGPLVAGIVDVFHREKLRVFGPSKAASQLEGS
                     KVFCKNVLHAADVPTAECRVFRDADGATRYITDRYQSDDE"
BASE COUNT          528 a         1170 c         1210 g          547 t
ORIGIN      
        1 ggcgtgggcg aggccggctc gctgcacctg gcccggaccg acaaagcctc ggagcaggat
       61 ttcgcgattg cctatgtctg cctgccggac gagcgggtgc tgcatttcat tcccggtttc
      121 gtctcagccg ccgagctgct cgcggagctg gagtgggccg agcggtgtta cgcggatgtg
      181 gcgaagctgc cggccgccga gcagccgctg gccgcgcggc gggcgcacct ggcggcgatt
      241 gcgaaggcgg acctgccgga attcgcgcgg cgcttcccgt cgcggtggca ggaggatagc
      301 ttgccggagg gcgaatcgac ggttgacttg cccaacgtcg tggcagcggc ccgggcgacg
      361 ttcgaatcga tgctgctgat gcggctggga ccgcaatggc gtcgcgacgc acaggccggc
      421 gggctcgcgc cgctggcggc tcacggcggc ctggccggcg agctggccca tttcgtcctt
      481 tccgagtatc cgctcgcggc gctgggcgac ctggccggcc ccgcctatgc ggcgtctagc
      541 gggcaacggt tctttaccta tcccaagcgg cgggccgacc tggccgcctg gtgggccctg
      601 gagcggagag cgaacaagcc gacactgctg gtcatcggcg acgacgacta cttcaccgcc
      661 tcgtcgtacc agccgtcaat tccgccgccg cgttccttgc gtagcaggat gtcggcgcgc
      721 gcgaacctgg tcgaatcgcc atctccgccg ctggcgccgg ccgccgatgc gccgccgttc
      781 gaatggaaaa aggcagcggc gggattgccc gacctctcca aattcgccgt gcaagtggtc
      841 acgctcgacg aactgagcct gcttctatcg gatgcgctct taccctcggt gagctacgtc
      901 cgcggccagc cgctgcggta cctcgtgttc gacgcgcgcg gttttcgcat cgcccagctt
      961 tcgagcgacg ccacgcctgc ccggctggcc gccgccatgc gagaagcgct tccgccggct
     1021 agcgcgaagc aaacgattgg agtaaccaga aatgcgaagc cttaaccgct gccgctggct
     1081 gtgcttgccg atcgccctgg cgctcgtcgc tccggtcgcc cgcggagatg aagagcccag
     1141 ccccgtccag gaaggccggc agctcttcga gcggaaattc acggcgcgaa acgccggaaa
     1201 tctgggcgac ggcctgggcc cgctcttcaa tcacgtctcg tgcgcggcct gccacgtgca
     1261 gggcggcctc ggcggcggcg ggccggtgga tgtgaacgtc gtgctcctct cggcgggcct
     1321 gcggtcctcg cggcccgaga agaagcaact gctcgccgcc ctccgcgagc aacatcccaa
     1381 cttcgtctcg cggagcgacg atatctcggc cacgatcatc ctgcaccgct tcagcaacga
     1441 ccccgcctac gaggtgttcc gctcgaaatt cgtgctcgag ccggtcccgt tggacccgac
     1501 cgccggcgag cgccggtcgc tgcagcagtc cctggcccgc actgccgaag cggaggtgaa
     1561 gacgccgccg cccctgcgcc tggtgacgac gcagcgcaac acgacggccc tgttcggcgc
     1621 ggggctgatc gattccatcc ccgacgggat gctccgcacc ctggcccagg agcagcagag
     1681 cgaggtttct ggccgcgtgc cgccgatcgg attcgacaga gtcggccgct tcggctggcg
     1741 gggacaaatc gagacgctgc acgacttcgt cctcggcgcg tgtgccaacg aactgggcct
     1801 ggaagtgccc ggccacgagc agccgcgcga tccccagcgg ccgaagtacc ggccggtggg
     1861 cttggacctt tcggcgagcg agtgccggtc gctgacggcg tttgtcgcgc agctgcccgc
     1921 gccgcgaatg gtcctgcccg ccgaccctga caagaaggcc ctggccgagc aagggcgcga
     1981 cctgttcggc aagatcggct gcgccgcgtg ccacgtggag cggatcgggc cggtgcaggg
     2041 aatttacagc gatctcctgt tgcacgacct gggcgaggcg ctgaccgacc ccgcgccggc
     2101 ggaggccacc ttcgtggcga cgggcaagcg cctgccgccg accgacgagt cgatcctggg
     2161 gaccaccgac ggcgaggcgc tcaagtcgtc gattcagccg cagccgccgc gtccgcgcgg
     2221 ctacaacggc ggcagccaat cgctggtcgc cgggccgagc gtcgtgctcg ttcccgataa
     2281 ggagacgcgg gaacggatcg aattccgcgc gcagccgacg ccggtcgagt cggaatggcg
     2341 gacgccgccc ttgtggggcg tggccgattc cgctccgtac ctgcacgacg ggcgggcggc
     2401 cagcctggtc gaggcgatcg cgctgcacgg cggcgaggcc gaaccctgca cgaagcggtt
     2461 cttcgcccag agcctgccgg agcggctggc ggtgctggaa tttctgggct gcctgcgcgc
     2521 gccgcagtag gtacggcagc gcgcactgcc atccgcttta cggcgagcgg ctggctcgct
     2581 cacaatggag gtgcggcggg cattcgtgga atgcacagga ggagcgagcg gcccatgacg
     2641 acagccacat tgaacgggag cgtctcgcaa atcggtgaag cggccggcgc ggtgtggcgg
     2701 acgctgagcg agcatgggcc gcagtcgctg gccaaaatcg tcgagcgcgt cggcggcaat
     2761 cgcgacctgg tgatgcaggg catcggctgg ctggcccgcg aaggcaagct ggaaatcgcc
     2821 gagacgcgcc gcgggcggat catctcgctg ctgcccgaat aaaaatctcg ggaggggcga
     2881 agctcctgct gagccttcgc ttttcgcgcg attcgcggct cggcgggagc cttgccctcc
     2941 ctgatcctcc ccattgggca acttccttgg tttgacgcgc tggcgtatat ttcagtcgta
     3001 ggaatctttc caccgacgtg gtgttttcat gagagtgctg gtcgtgggca gcggcgggcg
     3061 cgagcacgcg ctggcgtgga agatcgccgc tagcccgcgc gtggagcggg tcttcgtcgc
     3121 gcccggcaac gccggcacgg cggccgaagc cgaaaacgtg ccgatcgccg ccgacgacct
     3181 gccggggctg gtgaagttcg tcagggatgc ggccatcgac ctgaccgtcg tcggccccga
     3241 agggccgctg gtcgcgggca tcgtggacgt cttccaccgg gaaaaactgc gtgtttttgg
     3301 cccttccaag gccgccagcc agctcgaagg cagcaaggtc ttctgcaaga acgtgctgca
     3361 cgcggccgac gtgccgaccg ccgagtgccg cgtctttcgc gacgccgacg gcgcgacgcg
     3421 gtacatcacc gatcgctacc aaagcgacga tgaat
//
LOCUS       DASRJB010000018         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2262028, whole genome shotgun sequence.
ACCESSION   DASRJB010000018 DASRJB010000000
VERSION     DASRJB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262028"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFB80_00440"
     CDS             complement(<1..470)
                     /locus_tag="VFB80_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32243.1"
                     /translation="MPPLASLPRVAALLACTAAWPAAAADVAELDPAAVLAQHGLARV
                     DRVWVLADELRLRRDLAELPKRRERLLAVEHDLDQRIEKNRQRWQEAQPAIAALRQSL
                     ARLGSEDPQRQLLAQQIDALAAAAAEPTKIGAKSEVRSRVLVWIDERNSLASALA"
     gene            657..2546
                     /locus_tag="VFB80_00445"
     CDS             657..2546
                     /locus_tag="VFB80_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN32244.1"
                     /translation="MSGYRSLKRVLGESNLERKCRWLFGLCVGGLIILAFFSVGRIAE
                     NLIDDTAQYKGRGVARVYLYYVHWTGLTWRETKTEEEKKNLALQLKLQEDLVRKLDRE
                     ELQTRIIRTDDAQEPTRAGIRLEAPENDAERAILRSLRDEAEKRIVQGVQHGQPAGGA
                     ATPSAAAAFLESQQSRPLSRGFARPHEGKYIYYEVIDWTPYCISCHEGMHGSFAESAA
                     TSASYAGSVFDPRSTPFRVVRVTMPYAEIRQAVNRTRAILWSVGILTVFLAMVALYYV
                     VKYIVIKPLAHLRDVSDTVAKGDLEQRADIHTNDEFEELAASFNKMLRHLVETQSDLR
                     EANTELDGKVDELAHLNMQLHEMNRLKGEFLATMSHELRTPLNSIIGFSEVLQSLEAL
                     NDKQKRYALNIQVSGRKLLDMINDILDLAKMEAGKMDVRLSEFRIEALIAEQIDLVGA
                     LAEEKNIDLRTEIDRDLPLLYQDQAKVQQILTNLLSNAIKFTPEGGRITVGARGDSRG
                     RIEIWVADTGVGIPAAEKDIIFEKFRQGKAVLGRDNLTREYSGTGLGLSIVKELCKLL
                     GGEVTVESELGKGSTFRVILPWLRTETPLAASKLSAKLDDLTRPRRMEPKTDGAPEVA
                     ATGSP"
     gene            complement(2547..4967)
                     /locus_tag="VFB80_00450"
     CDS             complement(2547..4967)
                     /locus_tag="VFB80_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HZN32245.1"
                     /translation="MRFFDSRATAFLASVLVTLAGCGGPVSSPPAVTAVAAAAAPPPP
                     APAEAPLIGEPEAAPAELAPAVAEPAAPEPPSEPPTSDKELENALAAAEPQAAFQPPA
                     GPLKGEHPFRDRLRVPDFPAGMTWLNTKPLTKKDLAGKFVLLDFWTYCCINCMHILPE
                     LKKLEHEYPNDLVVIGVHSAKFEAEKITENIRSAILRNEIEHPVVNDKDHDIWNIYGI
                     SSWPTAILIDPQGMAVWGTRGEFKAADVKAKLDAALPFYRDNKLLDERPFKLDLESAK
                     EAAGPLSFPGKILADEKGQRLFISDSNHNRIVVAGLDGKLLDLIGAGPSGRSDGDFKT
                     ATFNHPQGCALVGNTLYVADTENHLLRKVDLQSKMVTTISGTGEQAASGFPGLDPAKT
                     AISREELPDRWVGPPQSTALNSPWALWVHKQDLYIAMAGPHQIWKMPLDEREIGPYAG
                     NGREDIVDGLLLPKVPYMLGFSSFAQPSGLSSDGTWLYVADSEGSSIRAVPLDPNPTK
                     RVRTVVGSAHLDSGRLFAFGDKDGAKAVAKLQHCLEVVYVGGPAASGKIYVADTYNHK
                     IKVVDAKTGETKTLAGNGQPGKADDPPQFHEPAGLAHVAGKLYVADTNNHLIRVIDLA
                     TGKVRTLTIAGLPAAGAASSSQPIAGGGGADAGADDPPPAPKAPAPKKPAFKGAHQEK
                     VPAATVQPVDGIVKLSVELKVPEGWHVNPLAPMSYWLDSPRAQGAADRAAFGRTKLAR
                     PVAKFEVPLKVAAAGEDEVQVSLDYYYCQDKDDGVCKVGSVVFTVPLKLATDGSKSPV
                     VLAHSILE"
     gene            complement(5064..>5396)
                     /locus_tag="VFB80_00455"
     CDS             complement(5064..>5396)
                     /locus_tag="VFB80_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32246.1"
                     /translation="WAAYLRTYDRIGNDPYVGHRLVSLLFEAGAKPRRNTWLFFGACA
                     GQPELLAAYVENLARILEGVREPILALGEFDAATFDSCLAGIRAWGLRPDAAYWYAVA
                     WAEGERPA"
BASE COUNT          900 a         1766 c         1818 g          912 t
ORIGIN      
        1 gccagggccg acgccaggct gtttcgttcg tcgatccaga cgaggacccg cgagcgaact
       61 tcgctcttgg cgccgatttt cgtcggctca gcggccgcag ccgccaacgc gtcgatctgt
      121 tgggccagca gttgccgctg cgggtcctcc gaccccagcc gggccagcga ctggcggagt
      181 gcggcaatcg ccggctgagc ttcttgccag cgctggcggt tcttttcgat ccgctggtcg
      241 aggtcgtgct cgacggccag tagccgctcg cggcgcttgg gcagttcggc caggtcgcga
      301 cgcagccgca actcgtcggc cagcacccac acgcgatcca cccgcgccag gccgtgctgc
      361 gccaggacgg cggcgggatc gagttcggca acgtccgctg ccgcggcggg ccaggcagcc
      421 gtacacgcca gcaaggcggc aacgcgcggc agactcgcga gaggaggcat ccctggaccc
      481 caacctggga cgagccgggc tagttcgatg ttccgacggt tgatcttagc cgctgaggcg
      541 ccagactgcc agcgctgctt ggacgggtgc ggcggtgacc gtctgctctg gctgcgccgg
      601 gggaatcgcc gtaaaatcaa gcggggccgg cagtttcttc caccaggcca gggctgatga
      661 gcggttatcg ttcgctaaag cgcgtgctcg gcgagagcaa cctggagcgc aagtgccgct
      721 ggctgtttgg cctgtgcgtg ggcggcctga tcatcctggc gttcttttcg gtcggccgca
      781 tcgccgagaa cctgatcgac gacacggccc agtacaaggg ccgcggcgtc gctcgcgttt
      841 acctgtatta cgtgcattgg acggggctca cctggcgtga aacgaagacc gaggaggaga
      901 agaagaacct ggccctgcag ctcaagctcc aggaggacct ggtccgcaag ctcgatcgcg
      961 aagaattgca gacgcggatc atccggaccg atgacgcgca ggagccgacg cgggccggga
     1021 tccggctcga agcgcccgag aacgacgccg agcgggcgat cctccgcagc ctgcgcgatg
     1081 aggcggaaaa gcggatcgtg cagggcgtgc agcacgggca gcccgcgggc ggcgcggcga
     1141 cgccgagcgc cgccgcggcg ttcctggagt cgcagcaatc gcggccgctg tcgcggggct
     1201 tcgcccggcc gcacgaaggc aaatacatct attacgaagt catcgactgg acgccctact
     1261 gcatttcctg ccacgaagga atgcacggca gcttcgccga gtcggccgcg acgagcgcat
     1321 cgtacgccgg cagcgtcttc gacccgcgga gcacgccgtt ccgcgtcgtc cgcgtgacga
     1381 tgccctatgc cgaaatccgt caggcggtga accggacgcg ggcgatcctc tggtcggtcg
     1441 gcatcctgac ggtcttcctg gcgatggtgg ccttgtacta cgtcgtcaag tacatcgtca
     1501 tcaagccgct ggctcacctt cgcgacgtga gcgacaccgt ggccaagggg gacctggagc
     1561 agcgggccga catccacacc aacgacgagt tcgaggagct cgccgcgtcg ttcaacaaga
     1621 tgctccgcca cctcgtcgag acgcagtcgg acctgcgcga ggccaatacg gagctggacg
     1681 gcaaggtgga cgagctggcc cacctgaaca tgcagctgca cgagatgaac cgcctcaagg
     1741 gcgagttcct ggccacgatg agccacgagc tgcgcacgcc gctcaatagc atcatcggtt
     1801 tctccgaggt gctccagagc ctggaggccc tgaacgacaa gcagaaacgc tatgccctga
     1861 atatccaggt ctccggccgc aagctgctgg acatgatcaa cgacatcctc gacctggcca
     1921 agatggaggc cggcaagatg gatgtccggc tctcggagtt ccgcatcgaa gcgctgatcg
     1981 cggagcaaat cgacctggtc ggcgcgctgg ctgaggagaa gaacatcgac ctgcggaccg
     2041 agatcgaccg cgacttgccg ctgctctatc aggaccaggc caaggtgcag cagatcctga
     2101 cgaacttgct gtccaacgcg atcaagttca cgcccgaggg cggccggatc accgtggggg
     2161 cccgcggcga ttcgcggggg cggatcgaaa tctgggtcgc cgacaccggc gtgggtattc
     2221 ccgcagccga gaaggacatc atcttcgaga agttccgcca gggcaaagcc gtgctcgggc
     2281 gcgacaacct gacccgcgaa tacagcggga cgggcttggg cctgtcgatc gtcaaggagc
     2341 tgtgcaagct gctcggcggg gaagtgacgg tcgagagcga gctgggcaag ggctccacgt
     2401 tccgcgtgat cctgccctgg ctgcggaccg agacgccact ggccgcttcc aagctcagcg
     2461 ccaagctcga cgacctcacg cgcccgcgac ggatggagcc caagaccgac ggcgcgccag
     2521 aggttgcggc caccggctcg ccgtagctac tccaagatcg aatgggccag cacgacgggc
     2581 gattttgacc cgtcagttgc cagctttaac gggacggtga acacgacgga gccgactttg
     2641 caaacgccgt cgtccttgtc ctggcaatag taatagtcga gcgagacctg gacctcgtcc
     2701 tcgcccgccg cggcgacttt tagggggact tcgaatttgg cgactggcct ggcgagcttc
     2761 gtccggccga aggcggcgcg gtcggccgct ccctgcgccc gcggcgagtc gagccaatac
     2821 gacatcggtg cgagtggatt gacgtgccag ccctccggca ccttcagctc cacgctgagc
     2881 ttcacgatgc catcgaccgg ctgaacggtc gcggccggga ctttctcctg gtgagcgccc
     2941 ttgaaggcgg gtttcttcgg cgcgggagcc tttggcgcag gcggcggatc gtcggcgcct
     3001 gcgtcagcgc cgccgccgcc ggcgatcggc tgcgacgaac tggccgctcc cgctgccggc
     3061 aggccggcga tcgtcagcgt cctgaccttg cccgtcgcca ggtcgatgac gcgaatcagg
     3121 tggttgttgg tatccgcgac atagagcttg cccgccacgt gggccagtcc cgccggctcg
     3181 tggaactggg gcgggtcgtc cgcttttccc ggctggccgt tgccggcgag tgtcttcgtc
     3241 tcgccggtct tggcatcgac gactttgatc ttgtggttat aggtgtcggc gacgtagatc
     3301 ttgccgctag cggccgggcc gccaacatag accacctcca ggcagtgctg gagctttgcc
     3361 acggccttcg cgccgtcttt gtcgccgaag gcgaacaggc ggccgctatc caagtgggcc
     3421 gagccgacga cggtccgcac ccgcttcgtc ggattcggat ccagcggcac cgcgcggatc
     3481 gagctgcctt cgctgtcggc gacgtacagc cacgtgccgt cggacgaaag accgctgggc
     3541 tgggcgaacg acgagaagcc cagcatgtac ggcaccttgg ggagcaagag gccgtccaca
     3601 atgtcctcgc ggccgttgcc ggcatagggg ccgatctcgc gctcatcgag cggcattttc
     3661 cagatttgat gtgggccggc catcgcgatg tacaggtcct gcttgtggac ccacagggcc
     3721 cacgggctgt tgagggccgt cgattgcggc gggccgaccc agcggtcggg caattcctcg
     3781 cgcgagatcg ccgtcttggc cgggtcgagc cccggaaagc cactcgccgc ctgttcgccg
     3841 gtgccggaga tcgtcgtcac catcttgctc tgcaagtcca ccttgcggag caggtggttc
     3901 tcggtatcgg cgacatacag cgtgttgccc acgagggcgc agccctgcgg atggttgaac
     3961 gtcgcggttt tgaagtcgcc gtcgctgcgg ccgctcgggc cggccccgat caggtcgagc
     4021 agcttgccgt cgagccccgc cacgacgatg cggttgtggt tgctgtcgct gataaacagc
     4081 cgctggcctt tttcgtcggc cagaatcttg ccgggaaagc tgagcggccc ggcggcctcc
     4141 ttggccgact ccaggtcgag cttgaagggc cgctcgtcaa gcagcttgtt gtcgcgatag
     4201 aagggcaagg ccgcgtccaa cttggccttc acgtcggcgg ccttgaactc gccccgcgtg
     4261 ccccacacgg ccatcccctg cgggtcgatc aggatcgccg tgggccagct gctgatcccg
     4321 tatatgttcc agatgtcgtg gtccttgtcg ttgaccacgg gatgctcgat ctcgttccgc
     4381 aagatcgccg agcggatgtt ctcggtgatc ttctcggcct cgaacttcgc agagtgcacg
     4441 ccgatcacca cgaggtcgtt cggatattcg tgctcgagct tcttcagctc cggcaggatg
     4501 tgcatgcagt tgatgcagca ataggtccag aagtcgagca gcacaaattt gcccgcgagg
     4561 tccttctttg tcagcggctt cgtgttgagc caggtcattc cggccggaaa gtccggcacc
     4621 cgcagccggt cgcggaacgg gtgttcccct ttgagcggcc cggccggtgg ctgaaatgcg
     4681 gcctgcggct ccgcggcggc gagagcgttt tccagttcct tgtcggatgt gggcggctcg
     4741 ctcggcggct ctggcgcggc aggttccgcc acggccggcg caagttcagc gggcgctgcc
     4801 tcgggctcgc cgatgagcgg ggcttccgcg ggggcaggcg gcggaggcgc tgccgcggcg
     4861 gccaccgccg tgacggccgg cggactgctg accggaccgc cgcagccggc aagtgtcacg
     4921 agcacgctgg ccaaaaacgc ggtcgccctg ctgtcgaaga acctcatgtc gcaacctcac
     4981 tcgagcgaaa attggcccca agttcgacct tttatcctac tgactggccg ggcgcggcga
     5041 aactcggatt cacggcaaaa gaactacgcc ggccgctcgc cctcggccca ggcgacggcg
     5101 taccagtacg cggcgtccgg ccgcaagccc caggcgcgga tgccggccag gcaggagtcg
     5161 aacgtggccg cgtcgaattc acccagggcg agaatcggct cccgcacccc ttcgaggatc
     5221 cgcgcgagat tctcgacata ggccgcgagc aattccggct gcccggcaca ggcgccgaag
     5281 aacagccacg tgttccgccg cggtttcgct ccggcttcaa acagcagcga gaccaggcgg
     5341 tggccgacgt acggatcgtt gccgatgcgg tcgtacgtgc gcaggtacgc ggccca
//
LOCUS       DASRJB010000019         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2827297, whole genome shotgun sequence.
ACCESSION   DASRJB010000019 DASRJB010000000
VERSION     DASRJB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827297"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFB80_00460"
     CDS             complement(<1..195)
                     /locus_tag="VFB80_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32247.1"
                     /translation="MSLLEFLLLLLIAGICGSVARAMAGYSHGGCLVSIALGFVGALI
                     GTWLARAANLPDPLTIQIGGH"
     gene            complement(291..965)
                     /locus_tag="VFB80_00465"
     CDS             complement(291..965)
                     /locus_tag="VFB80_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32248.1"
                     /translation="MPKFFQAGQQELQSNQWVSKHTADRPSFQCDINRGLFRSGHFQV
                     FAMPAGLLCLEMRHKDYGNIGQGGNNYAGMFFAMQFGAIGGLAAGLAAARDAERYGGT
                     PAQRWEKSFELMDESELLELARQRRKSFVCKLNEIRSVTINAPSLLSRTLGDNSLAGN
                     ITVRDKCMGKIVMQVRDQTHMAVAVEALAKRLGDRAKVNVEFDERTRRFRKRKGRSVV
                     GQSVGR"
     gene            complement(1119..1973)
                     /locus_tag="VFB80_00470"
     CDS             complement(1119..1973)
                     /locus_tag="VFB80_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32249.1"
                     /translation="MVGSRIWQLVLGCALILGLPAVSRGQALEELREDVRTNSPDPPR
                     EESPPAPRKPAYGCDHCEDDSDYGFGGLLLFAITAPFWGPPVWSGDSYSETGYFAHFP
                     YQHDDGYMIIGPALAEGELYRWSLRGRAEYGTDFRNLEWTGGHLLLDTSSRLGLEADF
                     RHVREDVPPGRFDSVWLGDGNIVFRFAQSELLVMRTGLGVTWLSDPADSNMGFNFTYG
                     GDFFPIQPWIFSGELDLGTLGHTSVVHFRLTTGVNLGMTEAFLGYDYYDVGRTQIAGL
                     VAGLRVWY"
     gene            complement(2054..3256)
                     /locus_tag="VFB80_00475"
     CDS             complement(2054..3256)
                     /locus_tag="VFB80_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32250.1"
                     /translation="MLHKSFFRSGHLPTLIAAFLYFDVSFMVWGLLGVLVTYIMAELP
                     LSPLEKGLLVATPTLGGALLRLPMGILADRWGGRRAGILGMGLTIVPLVLGWLWADSF
                     YELLLAGFLLGIAGASFAVALPLASRWYPLEYQGLALGIAGAGNSGTVVAALVAPRLA
                     EALGWHAVLAAAAGLVLVTLAAFYLLAKDSPNQPPPRSLADYARPLAHPDAWRFCLYY
                     CVTFGGFIGLASFLTLFFHEQYDLSRVSAGSLTALCVFSGSLLRPVGGWLADRLGGTK
                     LLVVVFAVVFVLMSGAATLPPLAVALTLIVVVMGLLGAGNGIVFQLIPQRFHREIGAM
                     TGIVGAAGGLGGFLLPSALGLAKGLTGSFSPGLAGFAAAALAAALLVWPAARAVLAEQ
                     RGLDAAAS"
BASE COUNT          653 a         1147 c         1149 g          482 t
ORIGIN      
        1 gtggccgccg atctggatcg tcagcgggtc gggcaggttg gccgcccggg ccagccaggt
       61 gccgatcagc gcgccgacga accccagggc aatcgacacc aggcaacccc cgtgcgagta
      121 gccggccatc gcgcgggcca cgctgccgca aatgccggcg atcaacagca acaggaggaa
      181 ttcaagtaac gacatgactg ggctcccaag aggcagacca gtcaggtgca agtggcgcgc
      241 caatccggtg agtcggtcga tcagtggtcg gtaggtcggt gtttggtcgt tcaccgacca
      301 accgactgac cgacaaccga ccgacctttc cgcttccgga accgccgcgt ccgctcgtcg
      361 aactcgacat tgaccttggc ccggtcgccc aggcgttttg ccaaggcctc gaccgctacg
      421 gccatatgcg tctggtcgcg aacctgcatg acaatcttgc ccatgcactt gtcgcggacg
      481 gtaatgttgc cggcgagcga gttgtcgccg agcgtgcggc tgagcaggct gggggcgttg
      541 atcgtgacgg agcggatttc gttcagcttg cagacgaagc tcttgcggcg ctggcgggcc
      601 agctccagca gctcgctctc gtccatcagc tcgaagctct tttcccagcg ctgggcgggc
      661 gtgccgccgt agcgctcggc gtcgcgggcg gcggccaggc ccgcggccag tccgccgatc
      721 gcgccgaact gcatcgcgaa gaacatccca gcatagttat tgcctccctg cccgatgttg
      781 ccgtagtcct tgtgccgcat ctccaggcag agcagcccgg ccggcatcgc gaacacctgg
      841 aaatgccccg agcgaaacag cccgcggttg atgtcgcact ggaacgatgg ccggtccgcc
      901 gtgtgcttgg agacccactg gttcgactgc agttcctgct ggccagcctg aaagaacttg
      961 ggcatgagcg gctcctcgaa tcgatgcgac ccgagtcaaa catagaacgc cgctttgggc
     1021 atgctacttc gaaatccgcg agcccgcgcg gacggcaaag tcggaaaata cggcggggcg
     1081 agccggtcgg tctgtcaccg actcaccgac cgaccggcct aataccacac ccgcaggccc
     1141 gccacgaggc cggcgatctg cgtgcgaccg acgtcgtaat agtcgtagcc gaggaaggct
     1201 tcggtcatgc ccagattgac gcccgtggtc aggcggaagt ggacgacgct cgtgtggccg
     1261 agcgtgccga ggtccagctc gccggagaaa atccagggct ggatcggaaa gaagtcgccg
     1321 ccgtacgtga agttgaagcc catgttcgag tcggccggat cggagagcca ggtgacgccc
     1381 aggcccgtcc gcatcacgag cagctcgctc tgtgcgaaac ggaacacgat attgccgtcg
     1441 ccgagccaaa ccgaatcgaa ccggcccggc ggcacgtcct cgcgaacgtg gcgaaagtcg
     1501 gcctccagcc ccagccgcga gctggtgtcg agcagcaggt ggccgccggt ccattccagg
     1561 ttgcggaagt cggtgccata ttcggcccgc ccacggagtg accagcgata cagctcgccc
     1621 tcggccagcg cggggccgat gatcatgtag ccgtcgtcgt gctgatacgg aaaatgcgcg
     1681 aagtagcccg tctcagagta gctgtcgccc gaccacaccg gcggacccca gaacggcgcg
     1741 gtgatcgcaa acaagagcag gccgccaaaa ccgtagtcgc tgtcgtcctc gcaatggtcg
     1801 cagccgtacg ctggcttccg cggcgccggc ggcgactcct cgcgtggcgg atcgggactg
     1861 ttagtccgca catcctcgcg cagctcctcg agcgcctggc cgcgcgagac cgccggcagg
     1921 ccgaggatca gcgcgcagcc gagcaccagc tgccagattc gactgcccac cataggaacg
     1981 ccccgcggtc tgccgctgcg aaaagcccgc ggacgatagt ccagacaggg cagtcggtca
     2041 aggtaagccg ctgctagctg gccgccgcat ccagcccgcg ctgttcggcg agaactgctc
     2101 gcgcggcggg ccagacgagg agagctgcgg cgagggccgc cgcggcgaat cccgcgaggc
     2161 ccgggctgaa cgagccggtg aggcctttgg cgaggccgag ggcgctgggg agcaggaagc
     2221 cgcccaggcc gccagcggcg ccgacgatgc cggtcatcgc gccgatctcg cggtgaaagc
     2281 gctgcgggat gagttgaaat acgatgccgt tgcccgcgcc taacaggccc atcacgacga
     2341 cgatgagcgt caaggcaacg gccagcggcg gcagtgtcgc cgcgcccgac atgagcacga
     2401 acactaccgc gaagaccacg accagcagct tcgtcccgcc gaggcggtcg gccagccagc
     2461 cgccgaccgg ccgcagcagc gagccgctga agacgcacag cgccgtcagc gacccggccg
     2521 acacccgact gaggtcgtac tgctcatgaa agaagagcgt caggaaactc gccaggccga
     2581 tgaagccgcc gaacgtcacg cagtagtaca ggcagaaccg ccaggcatca gggtgggcca
     2641 gcggccgcgc atagtcggcc aggcttctgg gcggcggctg atttgggctg tccttggcca
     2701 gcaggtagaa cgcggcgagg gtcaccagca ccagacccgc cgcggcagcc agaaccgcgt
     2761 gccagccgag cgcctcggcc agccgcgggg cgacgagcgc cgcgaccacc gtgccgctgt
     2821 tgcccgcgcc ggcgatgccg agtgccaggc cttgatactc gagcggatac cagcggctgg
     2881 ccagcggcag tgcgacggca aagctcgctc ccgcaatgcc cagcaggaag cccgccaaca
     2941 gcagctcgta aaacgaatcg gcccagagcc agccgagcac gagcggcacg atcgtcaggc
     3001 ccattcccag aatgcccgcg cgcctgccgc cccagcggtc agccagaatg cccatcggca
     3061 gccgcaacag cgcaccgccg agcgtgggcg tggcgacgag cagtcccttt tcgagcggcg
     3121 agagcggcaa ctcggccatg atgtacgtca cgaggacgcc cagcagcccc cagaccatga
     3181 agctgacgtc gaagtagagg aacgcggcga tgagcgtcgg caagtggccg gagcggaaga
     3241 aggatttgtg gagcatggga gtcggtggtc ggtggtcggt cggtcagaaa gtcggtggtc
     3301 ggtaagtcgg tggtcgagta gcctcacttg cctaccgact gaccgaccac tgactgaccg
     3361 accaccggtt ctacgctcac ggcgcagaac ttcagttccg gctgcttggc gacgcggccg
     3421 atggcgctga t
//
LOCUS       DASRJB010000020        10996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3391579, whole genome shotgun sequence.
ACCESSION   DASRJB010000020 DASRJB010000000
VERSION     DASRJB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391579"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..2083)
                     /locus_tag="VFB80_00480"
     CDS             complement(77..2083)
                     /locus_tag="VFB80_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN32251.1"
                     /translation="MAITRMLAFAWVCCATAAVLAQPYGQPDRGAPGDEMIQGYLARE
                     TAKLAAAYADDVKSLAAWEASRPEYLEQYYYMLGLSPRPERTPLEPKVTGTLKGDGYE
                     VDLMHYQSRPGLYVTGNLYRPAEVKPGERLPAVFYVCGHSQRGRDGNKVAYQSHGLWF
                     ARHGYICLVVDSLQLGEIAAIHHGTYHLNRWWWHSRGYTPAGVEAWNGVRGIDYLCSR
                     PDVDPQRIAVTGISGGGAATFWVAAADERVKVAVPVSGMADLESYVPNRVINGHCDCM
                     FLYNTFQWPWTRIANLVAPRPMLFVNSDKDAIFPMDANERISPRLERLYSLYGAGGDV
                     DAVVSVGGHAYRQDIRQAVYQFINAHLKGDARPITDSEIDIVSEGRDPGPYPIAPERL
                     RVFPTDADLPRDEVNTKIDQQFVPIAKNAAPKAGEFEAWKATLRAELRRVSFRYFPET
                     IPAAKKISDADALTERLESEEGVGFHLRFIGEKLPGPKNGMLLVVLGEEEAGTTPDWI
                     KNVPSDGRAVVLCEPRGIGATKWTRKNGPNYVERSHALLGRTVDTGRVWDVIAAAKFL
                     NVHYQSRPKIFVAGREGAGLIAAYAAALDDSIAGVTLIQPPATHMDSAAPQFLSVLRI
                     CDVPAALGLIAPRPLVVEGSPADVFAVTSAAYSAAGAGEKLSIK"
     gene            2234..5200
                     /locus_tag="VFB80_00485"
     CDS             2234..5200
                     /locus_tag="VFB80_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN32252.1"
                     /translation="MPAERTPHADDSLLTAPLLALTEWSLRAPLTVLFGAAALALLAV
                     AVTVNGLSFKTSRLDLLNPRSEYNRRWLAYLAEFGQRDDAVIVVRSDEAAALTAAIDD
                     LAKQLGPQPQFFESVFYRRDLSRLKDKALHYLPPEELAQLQERLRQAAAALPPAGQPA
                     DPAAALARLNDQLTHVSAATAEQRAAIQQQYARAIGPLVAALAGQGAAPPQSPAPTLP
                     AALNQFDPQYLQADNGRIGFVLTKLRQQPGESTPATAIAQLREIIRQAQSQHQRVWIG
                     LTGMPVIEYDEMQASQTDMVWTSVLSLGMVIALYLAAYGGLRHALLLNVVLVLATTYS
                     FGFATLAVGHLNILSSAFSAVLIGLGIDFAIHYVASYLKLRRQGLDEEESLLRTAVEV
                     GPGVVTGGVTTAAAFFMAAMTDFVGVRELGLIAGGSILLCVASAIVILPPLVLLVDRR
                     WPSRTTPEILPAGQWLQLPLCWPRMTLAASALATLVVGSGAVLLRYDHNLLNLQPRHL
                     ESADIERQLLNRMDDSVWFAVSVCSSRQELRERKAGFAALAGVAKTEEIGSLLPDASP
                     QQAERIAAVCRDLAAIPDRPPPPVPLDLAGLAREVTRAHQLLSQETPFETPATAALAQ
                     LRAALAAMQPAEADARLKQALPAMFAQVAGQLAPLRAIASPVPPRLDDLPPELVDRYV
                     GKNRTWLLKVYARGNIWNMQQLEEFVHAVESVDPRVTGHPVQTYYASRHMQSSYLWAG
                     LYALVAVLVLLWIDFRSLAHSLLAMLPLAVGVVLMCGTIGWLGLPLNPANMIVLPLIL
                     GIGVDHGVHLVHHWRQQSGRFELNDSTATAVLLTAATTTASFGALILARHQGLQSLGQ
                     VLTIGVTTCLAASIVMFPAVLAWLSEARQRGTRSAERGVAEEERWSDGEKERQSEDIV
                     AQAAPEPARAAPAPAEVPPLPLTPIPVTEEEIAALLDSALHPRLRQIAELPEDLPQAA
                     PLRRRAASQSNEGGVA"
     gene            complement(5256..8159)
                     /gene="uvrA"
                     /locus_tag="VFB80_00490"
     CDS             complement(5256..8159)
                     /gene="uvrA"
                     /locus_tag="VFB80_00490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN32253.1"
                     /translation="MDTSAAPAIPDDAVQGAEMLRVRGARTHNLRNIDLDIPRGRFVV
                     ITGPSGSGKSSLAFDTLFAEGQRQYIESLSTYARQFLSQLERPDVDSIDGLQPVICID
                     QRPGGANPRSTVATTTEIYDYLRLLFARLGEVSCHRCGQPIRQQSSQQIQDALMALPE
                     GTKVMLLAPLVRGRKGQHAEVFEQIRKAGQVRVRIDGAVVDLDAAGPLDGRKAHTIEA
                     VVDRIIIRPGTRSRLAESLELAIKQGEGVVVASYLNPAGEAAAKAAANGGENLWTDRL
                     FSTLYACPNCQLSVEEIEPRTFSFNSPYGACPTCEGLGYRLEFDPELLVPDLSRSIAG
                     GTVAAWRGNASGGRGSRRAANETGVKRQRQQIEQFLEAQKTPLDTPLSEMRPTVLEKL
                     FRGDGQEFPGLVTLLEQEYVTATDSERREQLESFRGHVSCAACGGTRLRPEALGVRIA
                     GRTIAEVCRLSIADARELFAGLAGQFSAEAAPVAEPILTEITARLGFLSKVGLEYLTL
                     DRPTDTLSGGELQRVRLATGIGSGLAGICYILDEPSIGLHPRDNQRLIDALRDLLARD
                     SSVLVVEHDEAMMRQADWLIDMGPGAGSRGGQVVAAGAPEEVVRNPDSITGRYLSGLE
                     RIEVPRERRKAAKTRSIVIEGVATNNLKSVAATFPLGLFIGVTGVSGSGKSSLINETL
                     GPALIRRLGGTAPKPGPHTALRGVSQIDKVIPIDQSPLGRSPRSNAATYTGAFDEIRK
                     VFAATKEARQLGFKASRFSFNAAGGRCEECQGQGQKRLEMNFLPDLFVRCPACEGRRF
                     NRQTLAVRYKGLSIADVLDLPIEEAVPLFENLPAIHRPLASLADVGLGYLRLGQAAPT
                     LSGGESQRVKLATELSRTDTGQTLYLLDEPTTGLHVGDIRLLLGVLQRLVDRGNTVIV
                     IEHHLDVLKCCDYLLDLGPGGGQHGGQLVATGTPEELAALPDNATGEFLRLVL"
     gene            8274..8429
                     /locus_tag="VFB80_00495"
     CDS             8274..8429
                     /locus_tag="VFB80_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32254.1"
                     /translation="MFVRPQSLIALALAFSASATALSADLLTIEEAQAKSKETGRPIL
                     AMGGSKT"
     gene            8445..9410
                     /locus_tag="VFB80_00500"
     CDS             8445..9410
                     /locus_tag="VFB80_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HZN32255.1"
                     /translation="MLERLSTDPSIATLVAQFVPLKVESDGPHWGEWSRKYSHEGNTI
                     PILFVIRADGEKLYGKSGAKQGDELPRFLLEHLRNAGTIFNDQQLATITTAVADADKA
                     LGEGDALTAVKRIESLRKLGTPGKFGSHATAAKQADDLYVKLVEQGRAALSDAQQKLD
                     GDDKFAGVFGVISANRIYGSLVELRKELASAERDLNKSPELKAHVSAAESLDRALTLA
                     SQKTPAVQKSGIAALGAIVSRSPDSPAAELAKARLAELGVQAPAPLPAKGRRTWTDAT
                     GKFKIEAELVRVADGKVELKKTDGTTISVPLDKLSAADQAIATGK"
     gene            9583..10851
                     /locus_tag="VFB80_00505"
     CDS             9583..10851
                     /locus_tag="VFB80_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZN32256.1"
                     /translation="MSTYPLIFRVRQTFQGPTLPIEQIPATVAAELSRLNLRQRIKPG
                     QSVAVTAGSRGVANIALIIKGAVDHLKSLGAAPFIVPAMGSHGGGTAEGQRELIEGYG
                     VTEQYCGCPIRASMETVVVCQAAEGFPVHFDKQAFGADHVLVVGRVKPHTNFVGDIES
                     GLMKMMLIGLGKHEGAKIYHRAILNYSFGQIVRSVAREVFSKCRIAAGIGIVENAYDK
                     TAKIQAVAPQEFEDREKELLVLAKEWIPRLPFKTCDILLLDEIGKNISGSGMDTNVVG
                     RKFNDHVAAEHEWPKIKRIIVRDLTEPTHGNATGIGMAEFCRTRCVERMDVRITRINC
                     LTGGHPTAAMLPLDYPSDRECLDAALPTIGLTEPPQARLLWAHNTLDVAEVECSVAYL
                     SEARERTDLEIIRDPRPLPLDAAGNLPDRV"
     gene            10859..>10996
                     /locus_tag="VFB80_00510"
     CDS             10859..>10996
                     /locus_tag="VFB80_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZN32257.1"
                     /translation="MKLSLLITCLGDVIRPDAGKAVVRILRRLGHEIDFPQAQTCCGQ
                     PM"
BASE COUNT         1788 a         3689 c         3691 g         1828 t
ORIGIN      
        1 caagtctagg tcactgcctg cccgccgctt tcggcagatt cgtcacaacc gatacagcgg
       61 gctgctgggg cgccgtttac ttgatcgaga gcttttcgcc agccccggcg gcggaatagg
      121 cggcggacgt gactgcgaat acgtcggcgg gcgagccttc gacgacgagc ggccgcggag
      181 cgatcaggcc gagcgccgcc ggtacgtcgc agattcgcag cacgctaagg aactgcggag
      241 cggcgctatc catgtgcgtc gcgggcggct gaatcagcgt gacgccggca atcgagtcgt
      301 cgagcgcggc agcataggcg gcgatcagtc ccgcgccttc ccgcccggcg acaaagatct
      361 ttggccggct ttggtaatgg acattgagga acttcgccgc tgcgatcacg tcccacacgc
      421 ggcccgtatc gaccgtgcgg cccaagagtg cgtgcgatcg ctcgacatag ttcggcccgt
      481 tcttgcgtgt ccacttcgtg gctccgatgc cgcgcggctc gcacaaaacg accgcccgcc
      541 cgtcggacgg aacgttcttg atccagtcgg gcgtcgtgcc ggcttcctcc tcgcccagta
      601 cgaccagcaa catgccattt ttgggtccgg gcagcttctc gccgataaac ctcaagtgga
      661 atccgacgcc ctcctcggat tcgagccgct ccgtcaaggc gtcggcgtcg ctgattttct
      721 tcgccgcggg aattgtctct gggaaatagc ggaagctcac gcgccgcagc tcggcgcgga
      781 gcgttgcctt ccacgcctca aactcgccgg ccttgggcgc cgcattcttc gcgatcggca
      841 cgaactgctg gtcgattttc gtgttgacct catcccgcgg cagatcggcg tcggtcggaa
      901 agacgcggag cctttccggc gcgatcggat agggcccggg gtcgcggcct tcgctgacga
      961 tgtcgatctc actgtcggta atcggccgcg cgtcgccttt caggtgcgcg ttgatgaact
     1021 ggtagaccgc ctggcggatg tcctggcgat aagcgtggcc gccgacgctg acgaccgcat
     1081 cgacgtcgcc gcccgcgcca tacaggctgt agagccgctc caggcggggg ctgatccgct
     1141 cgttggcgtc catcggaaag atcgcgtcct tgtcgctgtt gacaaacagc atcggacgcg
     1201 gagcgacgag gttggcgatc cgcgtccagg gccactggaa cgtgttgtag aggaacatgc
     1261 agtcgcagtg cccgttgatc acgcggttgg gcacgtacga ttcgaggtcg gccatgccgc
     1321 tgaccggcac cgcgaccttc acccgctcgt cggccgccgc gacccagaac gtcgccgcgc
     1381 cgccgccgct gatgccggtc acggcaatcc gctgcggatc gacgtccggc cggctgcaca
     1441 gatagtcgat cccgcggacg ccgttccagg cctcgacccc ggccggcgtg tagccccgcg
     1501 aatgccacca ccagcgattg aggtggtacg tgccgtggtg gatcgccgcg atctcgccca
     1561 gctgcagcga atcgacgacc aggcagatgt agccgtgccg cgcgaaccac aggccgtgcg
     1621 actggtaggc caccttgttg ccgtcgcgac cccgctggga atgtccgcag acataaaaca
     1681 cggccggcag cctctcgccc ggctttacct ccgccgggcg gtagaggttc ccggtcacgt
     1741 agagccccgg ccggctctgg tagtgcatca ggtcgacctc gtagccgtcc cccttgagcg
     1801 tgcccgtcac cttgggttcc agcggcgtcc gctcgggccg cggcgacagg ccgagcatgt
     1861 agtagtactg ctcgagatac tcgggccggc tggcctccca cgcggcgagc gacttgacgt
     1921 cgtccgcata ggccgctgcc agctttgccg tctcgcgggc gaggtacccc tggatcatct
     1981 cgtcgcccgg cgcgccgcgg tcgggctggc cgtagggctg tgccaacacg gcggcagtag
     2041 cacagcagac ccaagcgaat gcaagcatgc gggtgattgc catggctggt ctccggaaga
     2101 gcaggatggc tgatcgatgg gtgcggctca agtgctagca cgaattcgtc tttgccggag
     2161 cgtgccagcc tactaaatta cccgcacttt gtttcgcagc cccggcatga cgccggccga
     2221 tgcaggatct accatgccgg cggaaaggac tccgcacgct gacgattcgc tgctcacggc
     2281 gcccctcctg gcgctcacgg agtggtcgct ccgcgcgcca ttgacggtgc tcttcggggc
     2341 ggcggcgctg gcgctgctcg ccgtggccgt gacggtcaac ggcctctcat tcaagaccag
     2401 ccggctggac ctgctcaacc cgcggagcga atacaaccgc cgctggctgg cgtacctggc
     2461 cgagttcggc cagcgggacg acgcggtaat cgtcgtccgc agcgatgagg ccgcggcatt
     2521 gaccgcagcg atcgacgacc tggcaaagca gctagggccc cagccgcagt tcttcgaatc
     2581 ggtcttctac cgccgcgacc tgtcgcggct caaagacaag gcgctgcact atctgccgcc
     2641 ggaagagctg gcccagttgc aagagcggct ccgccaggcc gcggctgcct tgccgccggc
     2701 cggacagcca gccgatccgg cggccgcctt ggcccggcta aacgatcaac tcacacacgt
     2761 ctccgcggcg acggccgagc aacgggcggc gattcagcag cagtacgcac gcgccatcgg
     2821 gccgttggtt gcggcgctgg cagggcaggg ggcggcccca ccgcagtctc cagcgccgac
     2881 gctgcccgcc gccctgaacc aattcgatcc tcagtacctg caggccgaca atggccgcat
     2941 cggcttcgtc ctgacgaagc tccggcagca gcccggcgag tccacccccg ctaccgcgat
     3001 cgcccagctg cgcgagatca tccgccaggc gcagagccag caccagcggg tctggatcgg
     3061 cctcaccgga atgccggtga tcgagtacga cgagatgcag gccagccaga ccgacatggt
     3121 ctggaccagc gtgctcagcc tgggaatggt gatcgcgctc tacctggcgg cttacggcgg
     3181 gctgcggcac gccctactgc tcaacgtcgt cctggtgctg gccaccacat actcgttcgg
     3241 cttcgcgacg ctggccgtcg ggcacctcaa cattctctcc agcgcgttca gcgcggtgct
     3301 gatcggcctg gggatcgatt tcgcgatcca ctacgttgcc agctacctga agctccgccg
     3361 gcagggcctc gatgaggaag agtcgctgct gcggacggcg gtcgaggtgg ggccgggcgt
     3421 cgtcaccggc ggcgtgacca cggcggcggc gttcttcatg gcggcgatga ccgatttcgt
     3481 cggggtccgc gagctggggc tgatcgccgg cggcagcatc ctgctctgcg tcgccagcgc
     3541 gatcgtgatc ctgccgccgc tggtgctcct ggtcgatcgg cgctggccca gccgcaccac
     3601 gcccgagatt ctgccggcag gccagtggct gcagctgccg ctctgctggc cgcggatgac
     3661 gctggccgcc tcggcgctgg cgacgctggt cgtcggcagc ggcgcggtgc tgctccgtta
     3721 cgaccacaac ctgctcaact tgcagccgcg gcatctggaa agcgccgaca tcgagcggca
     3781 gctgctgaac cggatggacg acagcgtctg gttcgccgtg agcgtctgct cctcgcggca
     3841 ggagctgcgc gagcgaaagg ccggcttcgc ggcgctcgcc ggcgtcgcca agacggaaga
     3901 gatcggctcg cttctgcccg atgcctcgcc gcagcaggcc gagagaatcg ccgcggtctg
     3961 ccgcgacctg gcggcaattc cggaccggcc gccgccgccg gttccgctcg acctggcggg
     4021 cctcgcgcgc gaggttaccc gagcgcacca gctgctctcg caggagactc ccttcgaaac
     4081 gccggccacg gcggcgctgg cccagctgcg cgcggcgctg gcggcgatgc agccggccga
     4141 ggcggacgcg cgcctgaagc aggccctgcc cgccatgttc gctcaagtcg ccgggcagct
     4201 ggctccgctg cgggccatcg ccagcccagt gccgccgcgg ctggacgact tgccgccgga
     4261 gctcgtcgat cgctacgtcg gcaaaaaccg cacctggctc ttgaaggtct atgcccgcgg
     4321 caacatttgg aacatgcagc agctggaaga gttcgtccac gccgtcgaga gcgtcgatcc
     4381 gcgggtcacc ggccatccgg tgcagaccta ctacgcttcg cggcacatgc agagcagcta
     4441 tttgtgggcg gggctgtatg ccctcgtggc ggtgctcgtg ctcttgtgga tcgatttccg
     4501 cagcctcgcc cattcgctgc tcgcgatgct gccgctggcc gtgggcgtcg tgctgatgtg
     4561 cggcacgatc gggtggctcg gcttgccgct gaatccggcg aatatgatcg tgctgccgct
     4621 gattctgggc atcggcgtcg atcacggcgt gcacctggtg caccactggc gtcagcagag
     4681 cggccgcttc gagctgaacg attcgacagc gacggcggtg ctgctgacgg cggccacgac
     4741 gaccgcgagc ttcggggccc tgattctcgc ccgccaccag gggctgcaaa gcctcggcca
     4801 ggtgctgacg atcggcgtga cgacctgcct ggcggcgtcg atcgtgatgt tcccggcggt
     4861 gctggcgtgg ttgtcggagg cgcgccagcg cggaacgcgg agtgcggagc gcggagtggc
     4921 ggaggaggag agatggagcg atggagagaa ggagcgacag agcgaagaca tcgttgcgca
     4981 ggcggctccg gagcccgctc gggccgcgcc ggctccagcc gaagtgccgc cgctgccgct
     5041 gacgccgatt ccggttacgg aggaggagat tgcggcgctg ctcgactcgg cgctgcaccc
     5101 gcggctgcgg cagattgcag agctgccgga agacttgccc caggccgcac cgcttcgccg
     5161 ccgagcggcc tcgcaatcga atgagggagg ggtcgcctag tttctcaagg ccgtcccagg
     5221 cagcgtgggc ggggcaagcg gcgtgctcgc tcgttctaaa gcacaaggcg caggaattcg
     5281 ccggtggcgt tgtctggcaa ggcggcgagt tcttcgggtg tgccggtggc gacaagctgc
     5341 ccgccgtgct ggccgccgcc gggtccgagg tcgagcagat agtcgcagca cttcagcacg
     5401 tccaggtgat gctcgatcac gatcacggtg ttgccgcgat caaccaatcg ctggagcacg
     5461 ccgagcaaca aacggatgtc gccgacgtgc aggccggtcg tcggctcgtc gagcaggtag
     5521 agcgtctgcc cggtgtcggt gcgggacaat tcggtcgcta gcttcacccg ctgcgattca
     5581 ccgccgctca gcgtcggcgc tgcctggccc agccgcaggt agccgaggcc cacgtcggcc
     5641 aggctcgcca gcgggcggtg gatcgccggg aggttctcaa acagcggcac ggcctcttcg
     5701 atcggcaggt cgagcacgtc ggcaatcgag agccccttgt agcggaccgc gagcgtctgc
     5761 cggttgaagc ggcggccctc gcaggccggg cagcgcacaa acaagtccgg cagaaaattc
     5821 atctccagcc gcttctggcc ctgcccctgg cattcctcgc agcgcccgcc ggccgcgttg
     5881 aagctgaagc ggctggcctt gaatccgagc tgccgggcct ccttggtggc ggcgaagact
     5941 ttgcggattt cgtcgaacgc cccggtgtag gtcgcggcat tgctgcgcgg cgagcgtccc
     6001 agcggcgact ggtcgatcgg gattaccttg tcgatctgac tgacgccgcg cagggccgtg
     6061 tgcggtcccg gtttaggagc ggttccgccc agccggcgga tcagcgccgg cccgagcgtc
     6121 tcgttgatca gcgagctttt gcccgagccg ctgacgccgg tcacaccgat aaacaggccc
     6181 agtggaaaag tcgccgccac gctcttgaga ttgttggtcg cgacgccctc gatcacgatg
     6241 ctccgcgtct tggcggcctt gcggcgctcg cgagggactt cgatccgctc caggccgctc
     6301 aggtagcggc cggtgatcga gtccgggttc cggaccacct cttcgggagc tcccgcagcc
     6361 acaacctggc cgccgcggct gcctgcgccc ggccccatgt cgatcagcca atcggcctgc
     6421 cgcatcatcg cttcgtcgtg ctcaacaacc agcacgctgc tgtcgcgggc cagcaggtcc
     6481 cgcagggcgt cgatcagccg ctggttgtcg cgcgggtgca ggccgatcga cggctcgtcg
     6541 aggatgtagc agatgccggc caggccgctg ccgataccgg tcgccaggcg gacgcgctgc
     6601 agctcgccgc ccgacagcgt atcggtcggc cggtcgagcg tgaggtactc caagccgacc
     6661 ttgctcagga agccgagccg agccgtgatt tccgtgagga tcggctccgc caccggtgcg
     6721 gcctccgccg agaactgtcc ggctagccca gcgaacagct cgcgcgcatc ggcgatgctg
     6781 agccggcaaa cttcggcgat cgtccggccc gcgattcgca cgccgagggc ttcgggccgc
     6841 agccgcgtgc cgccgcaagc ggcacagctg acgtgcccgc ggaaggactc cagctgctcg
     6901 cgccgctccg agtcggtggc cgtgacgtac tcttgctcga gcagcgtcac gagcccggga
     6961 aattcctggc cgtcgccgcg aaagagcttc tccagcacag taggccgcat ttcggacagg
     7021 ggcgtatcga gcggcgtctt ctgcgcttcg aggaattgtt ctatttgttg ccgctggcgt
     7081 ttgacgccgg tctcgttggc ggctcggcgg gagcctcgcc ctcccgacgc gtttccgcgc
     7141 cacgccgcca ctgttccgcc ggcgatcgac ctggaaagat cgggcaccag cagctcggga
     7201 tcgaattcca ggcgatagcc caggccttcg cacgtcgggc aggcgccgta cggactattg
     7261 aaactgaagg tccgcggctc gatctcctcg acgctgagct ggcagttcgg gcaggcgtag
     7321 agcgtgctga agaggcgatc ggtccagaga ttctcgccgc cattcgcggc tgccttcgct
     7381 gctgcctctc cggccggatt gaggtagctg gccacgacca cgccttcgcc ctgcttgatc
     7441 gccagctcca gcgactcggc cagccgcgag cgggtgcccg gacgaatgat gatccgatcg
     7501 acgacggcct cgattgtgtg ggccttgcgg ccgtcgagcg ggccggcggc gtcgaggtcc
     7561 acgaccgcgc cgtcgatgcg gacgcggacc tgccccgcct tgcggatttg ctcaaacact
     7621 tccgcgtgct gccccttccg cccgcgaacc agcggcgcaa gcagcatcac cttcgtcccc
     7681 tccggcaggg ccatcagcgc gtcctgaatc tgctggctcg attgctgccg gatcggctgc
     7741 ccgcagcggt ggcacgaaac ttcccccagg cgggcaaaca gcagccggag gtagtcgtaa
     7801 atctcggtcg tggtcgcgac ggtgctccgc gggttcgcgc cgccgggccg ctggtcgatg
     7861 cagatcaccg gctgcaggcc gtcgatcgaa tcgacgtcgg gccgctcgag ctggctcagg
     7921 aactggcggg cgtaggtcga aaggctctcg atgtattgcc gctgcccctc ggcaaacagc
     7981 gtatcgaagg ccaggctgct cttgcccgag ccgctcggtc cggtaatgac gacgaaccgg
     8041 ccgcgtggaa tatccaggtc gatgttccgc aggttgtgcg tccgggctcc gcgaacgcgc
     8101 agcatttctg cgccctggac ggcgtcgtcg ggaattgcgg gtgctgcgga tgtgtccatg
     8161 cggcgccaat ctgcaaaggc caatcccgca gtatagccaa gctggaacgg tgacagcttt
     8221 gaagatgtga gggggtctgg tatagtcaat tcgccttttt caggagcgcg cctatgttcg
     8281 ttcgaccgca gtcactcatc gcactggcac tggccttcag cgcgtcggcc acggcgcttt
     8341 ccgccgacct gctgaccatc gaggaggcgc aggccaaatc gaaagaaacc ggccggccga
     8401 tcctggcgat gggcggctcc aagacgtgag ccccctgtca ggcgttgctc gaacggctca
     8461 gcaccgaccc ttcgatcgca acgctcgtgg cacagtttgt gccactcaag gtggaatcgg
     8521 acggtccgca ctggggcgag tggtcccgca agtacagcca cgaaggcaac acgattccga
     8581 ttctgttcgt gatccgcgcc gacggcgaaa agctctacgg caagagcggc gcgaaacagg
     8641 gagacgagct gccgcggttt ctgctcgagc acctgcgcaa cgccggcacg atctttaacg
     8701 accagcagct ggcgacgatc acgaccgccg tggccgacgc cgacaaggcg ctcggcgaag
     8761 gcgacgcgct aaccgccgtg aagcggatcg agtcgctccg caagctgggc acgcccggca
     8821 agttcggcag ccacgcgact gccgccaagc aggccgacga cctgtacgtg aagctcgtcg
     8881 agcagggcag ggcggcgctt tccgacgctc agcaaaagct cgacggcgac gacaaattcg
     8941 ccggagtgtt cggagtcatc tctgccaacc gcatctacgg ctcgctggtc gagctgcgca
     9001 aggagttggc ctcggccgag cgcgatctca ataagtcgcc ggagctgaaa gcccacgtat
     9061 cggcggccga gtcgctcgac cgggcgctca ccctggccag ccaaaagacg ccggccgtgc
     9121 agaagtcggg cattgccgcg ctgggtgcga tcgtttcgcg cagccccgac tcgcccgcgg
     9181 ccgagctcgc caaggcccgg ctcgccgaat tgggcgtgca agcgcctgcg ccgctgccgg
     9241 ctaaaggccg gcgcacctgg accgacgcca caggcaagtt caagatcgaa gccgagctgg
     9301 tccgcgtcgc cgacggcaag gtcgagctga agaagaccga cggcacgacc atctcggtgc
     9361 cgctcgacaa gctgagcgcc gccgatcagg ccattgcaac ggggaaatag gagcgaattc
     9421 ccgagtcgtg gcagcgaagg tcctgccgaa ctgcggcagc aataatctca cgcagaggca
     9481 cagagcattc tctcccttgt tgctcggtgt ttgttcctcc gtgccttggc gtcaaagagt
     9541 ttcgactgac agagatcgtc tgtgagctta taatcgagag ctatgtcaac gtatcctctc
     9601 attttccgcg tccggcagac ctttcagggg ccgacgttgc ccatcgagca gattcccgct
     9661 acggtcgccg cggaattgtc gcggctgaat ctgcggcagc ggatcaaacc gggacagtcg
     9721 gtggcggtca ccgccggcag ccgcggggtg gcgaatatcg ccctgatcat caagggggcc
     9781 gtggatcatc tgaagtcgct gggggccgcg ccgttcatcg tgccggcgat gggcagccac
     9841 ggcggcggca cggcggaagg gcagcgggag ctgatcgagg gttacggcgt aacggagcaa
     9901 tactgcggct gcccgatccg ggcgagcatg gaaacagtcg tcgtctgcca ggcggccgag
     9961 ggctttccgg tccacttcga caagcaggct ttcggcgcgg accacgtgct cgtcgtcggc
    10021 cgcgtgaagc cgcatacgaa tttcgtcggc gacatcgaat cgggcctgat gaagatgatg
    10081 ctcatcggcc tgggcaagca cgaaggagcg aaaatctatc accgggcgat tttgaattac
    10141 agctttggcc agatcgtccg cagcgtggcc cgcgaggtgt tctccaagtg ccggatcgcc
    10201 gcaggcatcg gcattgttga aaacgcctac gacaagacgg ccaagatcca ggctgtcgcg
    10261 ccgcaggagt tcgaggaccg cgagaaggag ctgctcgttc tggccaagga gtggattccg
    10321 cggctgccgt tcaagacctg cgacattctg ctgctcgacg agatcggcaa gaacatcagc
    10381 ggcagcggca tggacacgaa cgtcgtcggg cggaagttca acgaccacgt cgccgccgag
    10441 cacgagtggc ccaagatcaa acggatcatc gtccgcgacc tgaccgagcc gacccacggc
    10501 aacgcgacgg gcatcggcat ggccgaattc tgccgcacgc gctgcgtcga gaggatggac
    10561 gtgcggatca cgcggatcaa ctgcctcacc ggcggccatc cgaccgcggc gatgctcccg
    10621 ctggactatc cgagcgatcg cgagtgcctg gacgccgccc tgccgacgat cggcctgacc
    10681 gagccgccgc aggcccgcct gctctgggcc cacaacacgc tcgacgttgc cgaggtcgag
    10741 tgcagcgtgg cctatctgag cgaggcccgc gagcggaccg acctggaaat catccgcgac
    10801 ccccgtcccc tgccgctgga cgccgcgggc aacctgccgg accgggtgtg aggtccgagt
    10861 gaagctctcc ctcctcatca cttgcctcgg cgacgtcatc cggcccgatg ccggcaaggc
    10921 ggtggtgcgg attctgcggc ggctggggca tgagatcgac tttccgcagg cacagacctg
    10981 ctgcgggcag ccgatg
//
LOCUS       DASRJB010000021         6932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1981039, whole genome shotgun sequence.
ACCESSION   DASRJB010000021 DASRJB010000000
VERSION     DASRJB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981039"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFB80_00515"
     CDS             <1..671
                     /locus_tag="VFB80_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN32258.1"
                     /translation="LKLFLEEEDLHFYALIDASTSMEFGEPSKLQYAKQLAASLGFIG
                     LCRADRVKIESLGTSSRKPGPVLRGRASLWRMQEYLGGIESGENVSLLEGVKNFCLRN
                     SGKGILVLISDLMDKEGFDKALRYLVAQQMDVYVLHVMCPEELDPQVKGDLKLIDCED
                     EDVAEITVSRPLLDKYKRTLASFVDGAREFCTRRGMSYLMVSTETPVETLVAKYLRKR
                     GLVR"
     gene            786..2855
                     /locus_tag="VFB80_00520"
     CDS             786..2855
                     /locus_tag="VFB80_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HZN32259.1"
                     /translation="MLADLFIPMLTWWQWGLLGLIPPAIIALYFLKLRRQPLAVPSTY
                     LWSRAIEDLHVNSLWQRLRQSLLLFLQLLLIGLIAFTLLRPGQQGTELLGNRFIFLID
                     TSASMGATDVGPSRLDQAKKQAKNMIAQIKQGDTAMVISFSNVAKVEQSFTDNKKLLA
                     ARVDLIEQSFRPSDIGEALRAASGLANPGQSGDPNNPNDTPAAEAKPATMYMFTDGGF
                     APPKNFKLGNLEPIYLKQAGETQQNVAIVAFSTEVNPEKPGRVQAFGRFENHADAETT
                     IEASLFLNGTLLDAKKVSIGARDPKTKLPGVAGVNFDMQEIESGILKLQIDAKDHLAE
                     DNTAFAVVNLPRPVKVLVISPGTEALELALRTTEAQKIADITIAEPALLETKQYTDQA
                     AEGAYDLIVYDQCAPKTMPACNTLFIGRVPPGEELATPPPDGKTGAAKAPLKVPRWTA
                     GMRQGPPVIIDVDQVHPLTQLVQMGNVRVLEATPLKGPQGTVTLIDSDIGPLYAVGPR
                     AGYEDAVLGFDVVTHAPDGKAYANTDWPRRRSFPVFVMNVLKYLGGVRSSLAAPNILP
                     GEPAILRTATPVPTVLVSSPRGDRFTIPREVQNTFIFGRTDELGVYEVREGSGQKVAQ
                     QFAVNLFDTRESDLTPAEKLELGHTEVKAQETKQNARQELWKWLLLAAIGVLIFEWYI
                     YNRRVYL"
     gene            2958..4496
                     /locus_tag="VFB80_00525"
     CDS             2958..4496
                     /locus_tag="VFB80_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZN32260.1"
                     /translation="MLAKLHTFSLVGIEAVPVEVEVDVSPGALPKTILVGLPEAAVKE
                     STHRVERALANSGFIRPRDRVVVNLAPAELPKDAGSFDLPIALGTLAGSGQLASELFE
                     QYAVVGELALEGTTRPVKGALSMAIAAAKQRGLRGLVVPAESAGEAAVVEEIEAIPVA
                     SLAQAVGFFAGTLPIEPTPSRIGELFQELSKYDEDFGDVRGQEMAKRAIVVAAGGNHN
                     ILMLGPPGSGKTMLAKRVPTICPPLSAGESIETTRIYSSVGRLAPGQPLMARRPYRSP
                     HHTISEPGLVGGGNPPVPGEISLSHNGVLFLDELPEFNRRTLEVLRQPLEDGTVTISR
                     AMNATTFPADFMLVAALNPCPCGYRNDPRRECHCSIPQIEKYMAKISGPLLDRIDIHI
                     EVPAVPFKELSDDRAGTSSAQMREQVVAARVLQTARFANSRTRYNGQMTSRQIRQFCR
                     LSDDCQELLRASVNDLGLSARAHDKVLRVSRTIADLDRSEHIQASHLNEAINYRMLDR
                     QMWT"
     gene            4552..5166
                     /locus_tag="VFB80_00530"
     CDS             4552..5166
                     /locus_tag="VFB80_00530"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZN32261.1"
                     /translation="MPKRSSATSPYRCPWAVGDEYVAYHDLEWGVPVHDDRLLFEFLI
                     LEGAQAGLSWATILKKRQNYRQAFDDFDAAAVAKYGKRKQQTLLANPGIVRNRLKVAA
                     AIANAQAFLAVQEQHGSFDEYIWSFVGGQPLQNAWKSLKKVPARTPQSDAMSKELTRR
                     GFRFVGSTICYAFMQAVGMVNDHLVGCYRHAELRPQLPRRGRSP"
     gene            5205..5819
                     /locus_tag="VFB80_00535"
     CDS             5205..5819
                     /locus_tag="VFB80_00535"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HZN32262.1"
                     /translation="MNLLRSPWHIVFFVSFLIYLGIRSRYARQLRGVESVHRRVDGLE
                     VFLLVLVIGTSVLLPVLYLFTPLLAFADYRLPAFAPWCGAVLMIPALWLFWRSHADLG
                     RNWSATLELRKDHQLVTHGVYRLIRHPMYAAIFLFALAQAMLLSNWLAGWSAFVPFLL
                     MYVLRTPREERMMCEHFGQAYQDYMQRTGRLFPRLIGSREKRQE"
     gene            5874..>6932
                     /locus_tag="VFB80_00540"
     CDS             5874..>6932
                     /locus_tag="VFB80_00540"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN32263.1"
                     /translation="MLHRESILKRRDFFTTLLAGVALAQAQLSEARAQEAPREVSLER
                     PAEGRPHEGKVLLAVQAHSDDIPLTASGTVAKLIREGYTGYLVRATNDDMGDAPGLGT
                     PGTVGDHVLGNERDNAEVARVLGLKKVFDLNYPNHRMADVSQNELQCRLIFLIRLLKV
                     DTVVCWDPWAHDEENPDHWQIARGVEAACWMAGRAHDYPEQFAAGLAPHAVTQKYYFA
                     RRPEVTRVVDVSGVIEQVIDCNRANRAKGPAGVHGSRLKAELARRGQKLPLLGDDDVT
                     ADRNYIKEFLLTRRKNLGKQHGVIVDIRLHRRAPRRPAVDVVERLGVLHAVLFAEVLT
                     TSQQELLDVIAVGGDIVVA"
BASE COUNT         1284 a         2280 c         2196 g         1172 t
ORIGIN      
        1 acctgaagct ctttctggaa gaagaggatc tgcacttcta cgccctgatc gacgccagca
       61 cgagcatgga attcggcgag ccctcgaagc tgcaatatgc caagcagctg gcggcttcgc
      121 tgggcttcat cggcctgtgc cgggcggacc gcgtgaagat cgaatcgctg ggcactagca
      181 gccgcaagcc gggtccggtg ctccgcggcc gcgcgagcct gtggcggatg caggagtacc
      241 tggggggaat cgagtcgggc gagaacgtgt cgctgctgga aggggtgaag aacttctgcc
      301 tccgcaacag cggcaagggg attctcgttt tgatcagcga cctgatggac aaggagggtt
      361 ttgacaaggc cttgcggtat ctggtcgcgc agcagatgga cgtgtacgtg ctgcacgtga
      421 tgtgccccga agagcttgac ccgcaagtga agggggatct gaagctgatc gactgcgagg
      481 acgaggacgt agccgagatt acggtcagcc ggcccctgct cgacaagtac aagcgaacgc
      541 tggcctcgtt cgtcgatggg gcccgcgagt tctgcacccg ccgcggcatg agctatctga
      601 tggtcagcac cgagacaccg gtggaaacgc tcgtcgccaa gtacctgcgg aagcgcgggc
      661 tggtgcggtg acggaattca gaactcagaa tgccgaattc ggaaggatgc atttgctaca
      721 aacttttgtc agactggtgt aatctcttcg catctgaatt ctaaattctg cattctgtat
      781 tccccatgct cgccgacctc ttcatcccga tgctcacctg gtggcaatgg ggcctcttgg
      841 gcctcattcc gccggcgatc attgccctct atttcctcaa gctccgccgg cagccgctgg
      901 ccgtgcccag cacgtacctc tggagccggg cgatcgagga cttgcacgtc aactcgctct
      961 ggcagcggct gcggcaaagc ctgctcctct tcttgcagct actgctcatt ggcctgatcg
     1021 cgttcacgct gctgcggccc ggccagcagg ggacggagct gctcggcaac cggttcatct
     1081 tcctgatcga cacctcggcc agcatggggg ccaccgacgt cggcccgtcg cggctggacc
     1141 aggccaagaa gcaagccaag aacatgatcg cccagatcaa gcagggcgat acggcgatgg
     1201 tgatcagctt ctcgaacgtc gccaaggtcg agcagtcgtt taccgacaac aagaagctct
     1261 tggctgcgcg ggtcgatctg atcgagcaga gcttccggcc cagcgacatc ggcgaggcgt
     1321 tgcgggcggc ctcgggcctg gccaatccgg gccagagcgg cgacccgaac aatcccaacg
     1381 acacgcccgc ggccgaagcc aagccggcga cgatgtacat gttcaccgac ggcggcttcg
     1441 ccccgcccaa gaatttcaag ctgggcaacc tggagcccat ttatctcaag caggccggcg
     1501 agacgcagca gaacgtggcg atcgtcgcgt tcagcacgga agtcaatccc gagaagccgg
     1561 gacgcgtgca agccttcggc cggttcgaga accacgccga cgccgagacc acgatcgagg
     1621 cctcgttgtt tctcaacggc acactgctcg acgccaagaa ggtctcgatc ggggcgcgcg
     1681 accccaaaac gaagctgccc ggagtcgccg gcgtcaattt cgacatgcag gagatcgaga
     1741 gcggcatcct caagctgcaa atcgacgcca aggaccacct ggcggaggac aacacggcgt
     1801 tcgcggtcgt gaacctgccg cggccggtca aagtgcttgt gatctctccg ggcaccgaag
     1861 cgctggagct ggccctgcgg acgaccgagg cccagaagat cgctgacatc acaatcgccg
     1921 agccggccct cttggagacc aagcaataca ccgaccaggc ggcggaaggt gcttacgacc
     1981 tgatcgtcta cgaccagtgc gctcccaaga cgatgcccgc ctgcaacacg ctgttcatcg
     2041 gccgcgtgcc gccgggcgag gagctggcca cgccgccacc cgatggcaag actggagcgg
     2101 ccaaggcgcc gctgaaggtt ccccgctgga cggccggcat gcggcaaggc ccgccggtga
     2161 tcatcgacgt cgatcaggtc cacccgctga cgcagctggt gcagatgggg aacgtccgcg
     2221 tgctggaggc gacgccgctc aaggggccgc agggcaccgt gacgctgatc gattccgaca
     2281 tcggcccgct gtatgccgtc ggcccgcggg ccggctacga agacgcggtc ctcggcttcg
     2341 acgtcgtgac gcacgccccc gacggcaagg cctacgccaa caccgactgg ccccgccgcc
     2401 gcagcttccc ggtgttcgta atgaacgtgc tcaaatatct ggggggcgtc cgcagcagcc
     2461 tggccgcccc gaacatcctg cccggcgagc cggccatcct ccgcacggcg acgccggtgc
     2521 cgaccgtgct ggtcagctcg ccccgcggcg accgcttcac gatcccgcgc gaagtacaga
     2581 acacgttcat cttcggccgc accgacgagc tgggcgtgta tgaagttcgc gaaggctccg
     2641 ggcaaaaggt cgcccagcag tttgccgtca acctgttcga cacccgcgag agcgacctca
     2701 cgcccgccga gaagctggaa ctggggcaca ccgaggtcaa ggcccaggaa acgaaacaaa
     2761 acgcccggca ggagctgtgg aagtggctgc tgctcgccgc gattggcgtg ctcatcttcg
     2821 agtggtacat ctacaaccgg cgggtctatt tgtagagcct tccgtagccg aattcgccag
     2881 aattcggagt ggctgtgatc gtccgaactc tggcgagttc ggctacccac cttgatctgc
     2941 acccgtggac ggcccccatg ctcgccaagc tgcacacgtt ttcgctcgtc ggcatcgagg
     3001 ccgtgccggt cgaggtcgag gttgacgtct cgccgggagc cttgccgaag acgatcctgg
     3061 tcggcctgcc cgaagccgcc gtcaaagaga gcacgcaccg cgtcgagcgg gccctggcca
     3121 acagcggctt cattcgtcca cgggaccgcg tcgtggtaaa cctcgctccc gccgagctgc
     3181 ccaaagacgc cggctcgttc gacctgccga tcgcactggg cacgctcgcc ggcagcgggc
     3241 agctcgcgtc tgagctgttt gagcagtacg cggtcgtcgg cgaattggct ctcgaaggca
     3301 cgacccgtcc ggtgaagggc gcgctctcga tggcgatagc ggctgccaag cagcgcggcc
     3361 tccgcggact cgtcgtgccg gccgaaagcg cgggcgaagc ggcggtcgtc gaggagatcg
     3421 aggcgattcc cgtcgccagc ctggcccagg ccgtgggctt cttcgccggc acgctgccga
     3481 tcgagccgac gccctcgcgg atcggcgagc tgtttcagga gctctcgaaa tacgacgaag
     3541 atttcggcga cgtccgcggc caggaaatgg caaagcgggc gatcgtcgtc gcggcgggcg
     3601 ggaaccacaa catcctgatg ctcgggccgc ccggtagcgg caagacaatg ctggccaagc
     3661 gggtgccgac gatctgcccg ccgctctcgg cgggcgagtc gatcgagacg actcgcattt
     3721 acagctcggt cggccgcctt gcgccgggtc agccgctaat ggcccgccgg ccgtaccgct
     3781 cgccgcacca tacgatcagc gaaccggggc tggtcggcgg cggcaatccg ccggtgccgg
     3841 gcgaaatctc gctctcgcac aacggcgtcc tgtttctcga cgagctgccc gagttcaacc
     3901 gccgcacgct cgaggtcctg cggcagccgc tggaggatgg caccgtcacc atttcgcggg
     3961 cgatgaacgc cacgactttt cccgccgatt tcatgctcgt cgccgccctc aacccctgcc
     4021 cctgcggcta ccgcaacgac ccgcgccgcg aatgccactg ctcgatcccg caaatcgaga
     4081 agtacatggc gaagatttcc gggccgctgc tcgaccgcat cgacatccac attgaagtcc
     4141 ctgctgtgcc cttcaaggaa ctgtccgacg accgtgccgg aacctccagc gcgcagatgc
     4201 gcgagcaagt cgtcgccgcc cgcgtgctgc aaaccgcccg cttcgccaat agccgcacgc
     4261 gctacaacgg ccagatgacc agccggcaaa tccgccaatt ctgccggctt agcgacgact
     4321 gccaggagct gcttcgcgcg agcgtcaacg acctcggcct ctcggcccgg gcccacgaca
     4381 aggtcctccg cgtcagccgc acgatcgccg acctcgaccg gagcgagcac atccaggcgt
     4441 cgcacctgaa cgaagcgatc aactaccgca tgctggaccg gcagatgtgg acgtgaggcg
     4501 gcagcgacag acgcgcgacg aacgaactta agccgtgcca cggaaagagg tatgccaaaa
     4561 cgcagctcag cgacttcgcc ataccgctgc ccgtgggcgg tcggcgatga gtacgtcgcg
     4621 taccacgacc tcgaatgggg cgtgccagtg cacgacgatc ggctgctgtt tgagtttctg
     4681 atcctcgaag gggcgcaggc gggtttgagc tgggcgacga tcctgaaaaa gcgccagaac
     4741 tatcggcagg cgttcgacga tttcgacgcg gcggccgtgg ccaaatacgg caagcggaaa
     4801 cagcaaaccc tgctggcgaa tcccggaatt gtccgcaatc gcctgaaagt cgcggccgcc
     4861 atcgccaacg cccaggcctt tctcgcggtt caagaacaac acggctcgtt cgacgagtac
     4921 atctggagct tcgtcggtgg gcagccgctc cagaatgcct ggaagtcact gaaaaaagtg
     4981 ccggctcgca ctccgcagtc ggatgccatg agcaaggagc tgactcgccg cggcttccgg
     5041 tttgtcggca gtacgatctg ctacgcgttc atgcaagccg tgggtatggt caacgaccat
     5101 ctcgtcggtt gctaccggca cgcagaattg cggccgcaac ttccccgtcg cggaagatcg
     5161 ccctgacagc ccttgggcga tgctctcggg taaaacttga ctccatgaac ctccttcgat
     5221 ctccctggca catcgtcttc ttcgtttcct tcctgatcta cctgggaatt cgcagtcgct
     5281 atgcgcgcca actgaggggc gttgaaagcg tgcaccggag ggtagatggg ctggaggtgt
     5341 tcctgctggt gctggtgatc gggacaagcg tgctgctgcc cgtgctgtac ctgttcacgc
     5401 cgctattggc gtttgccgat taccggctgc cggcattcgc accgtggtgc ggggccgtat
     5461 tgatgattcc ggcgctgtgg ctcttctggc gctcgcacgc cgatttgggg cgtaactggt
     5521 cggcgacgct ggagctgcgg aaggaccacc aactcgtcac gcacggcgtt taccggctga
     5581 ttcgtcatcc gatgtatgcc gccatcttcc tgttcgccct ggcccaagcc atgctgctct
     5641 ccaactggct cgccggctgg tccgcgttcg tcccgttctt gctgatgtac gtgctccgca
     5701 cgccccgtga agagcggatg atgtgcgagc acttcgggca ggcctaccag gactacatgc
     5761 agcgtaccgg ccgcctgttc ccccgcctca tcggcagccg ggaaaaacgt caggaataaa
     5821 ctccgtggga tgacgcccgc gacttactct gccgcctgtt caaggagaat gccatgctcc
     5881 accgcgaatc gattctcaaa cgccgcgact tcttcacgac actgctggcc ggcgtggctc
     5941 tggcgcaggc gcagctcagt gaagcgcggg cgcaggaagc gccgcgcgaa gtgtcgctgg
     6001 aacgccccgc cgaggggcgg ccgcacgagg gaaaagtgct gctcgcggtc caggcgcact
     6061 cggacgacat tccgctcacg gcgtcgggga cggtcgcgaa gctgatcagg gagggctaca
     6121 cgggctacct ggtgcgggcc acgaacgacg acatgggcga tgctccgggg ctgggcacgc
     6181 cgggcaccgt cggcgatcac gtgctgggca acgagcgcga caacgccgaa gtggctcgcg
     6241 tgctggggct gaagaaagtc ttcgacctca actatcccaa ccaccgcatg gccgacgtct
     6301 cgcaaaacga gctgcaatgc cggctgatct tcctgatccg gctgctgaag gtcgatacgg
     6361 tcgtctgctg ggatccgtgg gcccacgacg aggagaaccc cgaccactgg cagatcgccc
     6421 gcggagtcga ggcggcctgc tggatggcgg gccgcgccca cgactatccc gagcagttcg
     6481 ccgccggcct cgcgccgcac gcggtgaccc agaagtatta ctttgcccgc cggccggaag
     6541 tcacccgcgt cgtcgatgtc agcggcgtga tcgagcaggt catcgactgc aatcgggcca
     6601 accgggccaa agggcccgcc ggggtgcacg gttcgcggct gaaggccgag ctggcccgcc
     6661 gcggccagaa gttgccgcta ctcggcgacg acgatgtcac cgccgaccgc aattacatca
     6721 aggagttcct gctgactcgt cgtaagaacc tcggcaaaca acacggcgtg atcgtcgata
     6781 tacgtctcca ccgccgagcg ccgcggcggc cggctgtcga tgtagtggaa cgcctcggcg
     6841 tactccacgc cgtattgttt gccgaggttc ttacgacgag tcagcaggaa ctccttgatg
     6901 taattgcggt cggcggtgac atcgtcgtcg cc
//
LOCUS       DASRJB010000022         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1134888, whole genome shotgun sequence.
ACCESSION   DASRJB010000022 DASRJB010000000
VERSION     DASRJB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134888"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFB80_00545"
     CDS             complement(<1..182)
                     /locus_tag="VFB80_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32264.1"
                     /translation="MPRPARSFLQATGRDVRGRKQFRYHNDWWQVRDEATFAGPRSPG
                     PCRESGGRWRPISRAAA"
     gene            complement(441..647)
                     /locus_tag="VFB80_00550"
     CDS             complement(441..647)
                     /locus_tag="VFB80_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32265.1"
                     /translation="MAYHPLRLHPDTVARQQMLARLLRRCESSQRSAADSDAADDSRP
                     ARSAAEATCEAGGTAIGWGPGREK"
     gene            complement(812..1678)
                     /locus_tag="VFB80_00555"
     CDS             complement(812..1678)
                     /locus_tag="VFB80_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZN32266.1"
                     /translation="MRIVVLCTIAISTLGLAVRGGAAEPAEIKAGLAKALTLHASFDK
                     SLEADFARGDKTCYVQQGTNLVAAAPTAEVRLAPDGGRFGGGLHFTKKNNFRPAFKDA
                     GVLGYNAKNWNATVSVWLRLDPDKDLEPGYCDPVQIVGDDSKKGFIFLEWSKDETPRH
                     FRYAIRPLFPIWNPDNLPWDQIPAQKRPMVQVERAPFSREKWTHAVFTLQNVNDKSQP
                     QSGRLYLNGQLQGTIEKWDLTFGWDPARVLLVLGASYVGYLDDLAVFNRTLTEEEVQQ
                     LFGLAKGAGELR"
     gene            complement(1708..2598)
                     /locus_tag="VFB80_00560"
     CDS             complement(1708..2598)
                     /locus_tag="VFB80_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN32267.1"
                     /translation="MNPAAREFVLDPAAYGLPTRDDLVQLRIWDMHYHGFYPGGIKQH
                     EENLFYVERMGIERMLSLDIAGTPTDPLGNSQSAEQQREIREYLEKHADRVSGLIPVD
                     PSQPVESCRKIEEWIRRGPCVGIKYHGGNPGGVVCSHPDNDAILRLAAELKALVYIHT
                     WMKVGGQPRRAGGDNQRGESTPQDVALLAARFPDVPLICGHSGGDWELGVRAVRPHDN
                     VYIEFSGSDPHSGQVDFTVRELGASRLIWGGHGPSRSYSTELSKVLDADLTREQRLQI
                     FGGNLRRIAAPIFREKGYKL"
     gene            complement(2617..>3061)
                     /locus_tag="VFB80_00565"
     CDS             complement(2617..>3061)
                     /locus_tag="VFB80_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32268.1"
                     /translation="LAAAQKRGLVVQIVMRLEDERVHHPAINVPLVNVAPLVGALKKA
                     SEAKVQLINSAGPLLGNKVADLVRQTQVTFDIAATEGNGGVGRLIEGKNYSYRGAIPA
                     ERLLFGSHAPYFPCESALLKLFESPLTREQLQSLMNANARRLLSQ"
BASE COUNT          486 a         1042 c          981 g          552 t
ORIGIN      
        1 gccgcggcgc gcgagatcgg ccgccacctt ccgccggatt cgcggcaggg ccctggcgaa
       61 cgcggtccgg cgaacgtcgc ttcgtcgcga acctgccacc aatcgttgtg atagcggaat
      121 tgtttgcggc cgcggacgtc acggccggtg gcttgcaaaa atgaccgcgc gggtcgcggc
      181 aaatccagac ctcttcccag gccggcggaa tggcgagcgt acggattcgc tccaagatct
      241 tccggtcgcg aacgacttgc ccgcgctgat tcaattagcg caacctccgg ccgtaccgct
      301 gccgccggat tcccgcctgc gagtcgctca cccaccgcag ccccatccgg cgcgcggccc
      361 gccgcggttc cgcggcgtca gatgccttcc tccgctccac aacgatcact ttccgctagc
      421 atccgtcccg cccaatggcc tcatttttcg cggcctggtc cccagccgat tgccgtgccg
      481 ccggcttcgc aggtggcctc agctgcggag cgggccggcc tcgagtcgtc ggccgcgtcg
      541 ctgtccgctg ccgaacgctg cgatgattcg cagcgcctca gcagccgggc cagcatctgt
      601 tggcgggcga cggtgtccgg atgaagccgc agcggatgat aagccattgc acttttttcg
      661 gggcgagcgg acatgggaca aagaacgata tccctcgcct tatgcaagca gcgtgccgga
      721 ggccggcagc ggaaaaatct cgcttgccgc tcagaaccaa aggacactcg ctgcgaccat
      781 cgcgacgatt cggcactgtg gcgtgccact gctacctcag ctcgccagcg cctttcgcca
      841 gcccgaagag ctgctgcacc tcttcctccg tgagcgtccg gttgaacacc gccaggtcat
      901 cgaggtatcc aacgtacgaa gcccccagca ccaagaggac gcgggccgga tcccagccga
      961 acgtcaggtc ccatttctcg atcgtgccct ggagctggcc attgagatag agccggccgg
     1021 actgcggctg gctcttgtcg ttgacgttct gcagcgtgaa gacggcgtgt gtccattttt
     1081 cacgggagaa cggcgctcgc tcgacttgca ccatcggacg tttctgcgcg ggtatctggt
     1141 cccacggcag gttatcggga ttccagattg gaaacagcgg gcggatggca tagcggaagt
     1201 gccgcggcgt ttcgtccttc gaccactcca ggaagatgaa gcccttcttg ctgtcgtcgc
     1261 ccacgatctg caccggatcg cagtagcccg gctccaggtc cttgtcggga tcgagccgca
     1321 gccagaccga aacggtcgcg ttccagtttt tggcgttata gcccagtacg ccggcgtcct
     1381 tgaacgccgg gcggaagttg ttcttcttcg tgaagtgcag cccgccgcca aaccggccgc
     1441 catcgggcgc gagccggacc tcggctgtgg gcgcggccgc gaccaggttc gtcccttgct
     1501 ggacgtagca cgtcttgtcg ccccgcgcga agtccgcctc caaggacttg tcgaacgagg
     1561 cgtgcagcgt caacgctttc gccagtccgg ctttgatttc cgcgggctcc gccgccccgc
     1621 cgcggaccgc caggccgagg gtgctgatcg cgatcgtgca gaggacgacg attctcatgg
     1681 tttggctccc aggcaaatcc tgccggctca caacttgtat cccttctccc ggaaaatcgg
     1741 cgctgcaatc cgccgcaggt tcccgccgaa gatctgcagc cgctgctcgc gcgtcaggtc
     1801 cgcgtccagg accttcgaca gctccgtcga gtacgaccgc gagggtccgt ggccgcccca
     1861 gatcagccgg ctcgcgccca attcgcggac cgtgaagtcg acctgcccgg agtgcgggtc
     1921 cgagccgctg aactcgatgt agacgttgtc gtgcgggcgg acagcccgca cgcccagctc
     1981 ccaatcgccg ccggagtggc cgcagatgag cggcacatcc ggaaaccgcg cggcgagcag
     2041 cgccacgtcc tgcggcgtcg attcgccgcg ctggttgtcg ccgccggccc gccgcggctg
     2101 cccgccgacc ttcatccagg tgtgaatgta gaccagggcc ttcagctccg cggccagccg
     2161 gaggatcgcg tcgttgtccg gatggctgca tacgacgccg ccggggttgc cgccgtggta
     2221 cttgatgccc acgcacggcc cgcggcgaat ccactcttcg atcttgcggc agctttcgac
     2281 gggctgcgaa ggatccaccg ggatcagccc ggacacgcgg tcggcatgct tctcgaggta
     2341 ttcgcgaatc tcgcgctgct gctcggccga ttgcgaattg cccaaaggat cggtcggcgt
     2401 gccggcgatg tcgagcgaga gcatccgctc gatccccatc cgctcgacgt agaacaggtt
     2461 ctcctcgtgc tgcttgatgc cgccggggta aaacccgtga tagtgcatgt cccagatccg
     2521 cagctggacc aggtcgtcgc gcgtcggcag gccgtaggcg gccggatcga ggacgaactc
     2581 gcgagcggcg ggattcatgg ggcgtgctct cggcggctac tggctcagca ggcggcgggc
     2641 gttggcgttc atcaggctct ggagctgctc gcgcgtgagc ggcgactcga acagcttcaa
     2701 gagggcgctt tcgcacggga aatagggggc gtgcgagccg aacagcagcc gctcggcggg
     2761 aatcgcgccg cggtagctgt agttctttcc ttcgatgagc cgccccacgc cgccgttgcc
     2821 ttcggtggcg gcgatgtcga aggtgacctg cgtctggcgg accaggtcag cgactttgtt
     2881 gcccagcagc ggcccggccg agttgatcag ctgcactttc gcctccgagg ccttcttcag
     2941 cgcgccaacg agcggcgcga cattgaccag cggcacattg atcgcgggat gatgaacgcg
     3001 ctcgtcttcc agccgcatca cgatctggac cacgaggccg cgcttctgtg ccgcagccaa
     3061 t
//
LOCUS       DASRJB010000023         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2263672, whole genome shotgun sequence.
ACCESSION   DASRJB010000023 DASRJB010000000
VERSION     DASRJB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263672"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFB80_00570"
     CDS             complement(<1..445)
                     /locus_tag="VFB80_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZN32269.1"
                     /translation="MSTAAHSRAAALAPAGGLRLTQPAALVAYLAVCGLAAWLVLPPD
                     PAALWTIAAGIVCNASCAILGCYLVLRRMSLLGDAISHAVLPGLAIGFLISGSLTSPW
                     ILVGAMVLGVLTSVLTQGISSLGRVSEDASLGVVFTSLFALGVILI"
     gene            complement(442..1767)
                     /locus_tag="VFB80_00575"
     CDS             complement(442..1767)
                     /locus_tag="VFB80_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZN32270.1"
                     /translation="MIDWLLQYARLNYNLLVVLAGTSLLGIGAGIIGAFAVLRKRALT
                     GDALSHAALPGLCLAFLIVRERDLIALLLGALASGVVGIAIISALGRWTRVKNDAAIG
                     TVLTVFFGLGVVLLKIIQNLPGVGSRAGLNSYIFGKTAGMTQGDVYVILAAAALCLAL
                     IVVFYKEFQLVSFDAEFAQSLGWPVYGVDLLLMSLVAAAVVIGLPSVGVILVSALLIM
                     PGAAARFWTDRLGTLLLLAGGFGFSMGFVGTLLSAKFADLPTGPIIVLTGAALFLVSL
                     LFGVRRGALARLLEHLRFQRQWHERQLLRTIYDVLEGSRTRQSSDALQGPNSGEFGYV
                     TPAALLARRVWTLPQLSGMLAAAAADNLLTVRGDQIALTPMGARQAVEVARGQRLWQE
                     FLTEYPEQAGSVVNLASASIADFVPAATVSALTQKLTAEGRWPREEHLA"
     gene            complement(1764..2528)
                     /locus_tag="VFB80_00580"
     CDS             complement(1764..2528)
                     /locus_tag="VFB80_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZN32271.1"
                     /translation="MTTILDIHDVTVAYHRRPVLWDVDLTLKQPQLAAIVGPNGAGKS
                     TLIKAVLGLVPMASGSVHVFGQPVARVRKRIGYVPQRESVDWDFPVSVLDVVLMGTYG
                     KLGWIRRPGAAERDWARKCLDKVGLVSYERQQIGQLSGGQQQRVFLARALAQKADIYF
                     MDEPMAGVDAATERMIFQVLEDLRQQGKTIIAVHHDLRTVPQYFDYVVLLNVRLVAAG
                     PRELVFTPENLRKTYGGRLAVLDAASEAVEAETRAL"
     gene            complement(2623..3531)
                     /locus_tag="VFB80_00585"
     CDS             complement(2623..3531)
                     /locus_tag="VFB80_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZN32272.1"
                     /translation="MSAVIAGCDSSGATTQVAAPKQFSGKYPIQVVCTTGMVADLAKN
                     IGGEHVAVTALMGAGVDPHLYTASPADVSQLSKADIILYSGLHLEGKLAELLGRMGQR
                     KPTTAVAEKIPHEKIISDPGGAHDPHVWFDVSLWSVAAESAADALIAFDPPHAETYRA
                     NLKAYQARLAELHAYAGEQMRSVPKEQRVLVTAHDAFHYFGNAYETEVRGIQGISTTS
                     EAGVRRIRELVDFLVERKIKAVFVETSVSDQNIRSLLEGAAARGHTVVIGGTLFSDAM
                     GDEGTPDGTYEGMVRHNVDTIVKALK"
     gene            3876..>4042
                     /locus_tag="VFB80_00590"
     CDS             3876..>4042
                     /locus_tag="VFB80_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32273.1"
                     /translation="MCRTRRCRHGFTVVEMLVVIAIIAVMAGLLLPAVQMAREAARRT
                     QCQNNLKQLALA"
BASE COUNT          736 a         1357 c         1294 g          655 t
ORIGIN      
        1 tgatcaggat cacgcccagg gcaaacagcg acgtgaatac gacgcccagg ctggcgtcct
       61 ccgaaacgcg gcccaggctg gagatgccct gcgtcagcac gctcgtcagc acgcccagca
      121 ccatggcgcc gacgagaatc caggggctgg tcagcgagcc ggaaatcaga aagccgatcg
      181 ccagccccgg caatacggca tggctgatgg cgtcgcccag caggctcatc cgccgcagca
      241 cgagatagca gcccaggatg gcgcagctcg cgttgcaaac gatgcccgcg gcgatcgtcc
      301 acagcgcggc gggatcgggc ggcagtacga gccacgccgc cagtccgcag accgccaggt
      361 aggccactag cgccgccggc tgcgtcaggc gcaggccgcc ggccggtgca agggcggcgg
      421 ctcggctgtg ggcggcggtg ctcatgcgag gtgctcctcg cgtggccagc ggccttcggc
      481 cgtgagtttt tgtgtcagcg cggagaccgt ggccgccggg acaaagtcag cgatcgaggc
      541 gctggccaga ttgacgacgc tgccggcctg ctcgggatac tccgtcaaga actcctgcca
      601 caatcgctgg ccgcgggcga cttcgacggc ctgtctcgcg cccattggcg tgagcgcgat
      661 ttgatcgcca cgcacggtca gcaggttgtc cgcggcagcg gcggccagca tgccgcttag
      721 ctgtggcagc gtccagacgc ggcgggcgag caaggccgcc ggcgtcacgt agccgaattc
      781 gccagaattc gggccctgga gcgcgtccga actctggcgc gttcggctac cctccagcac
      841 gtcatagatc gtccgcagca gctgtcgctc gtgccactgc cgctgaaaac gcaagtgctc
      901 cagcagccgg gccagcgcac cgcgccggac gccgaagagc agcgaaacca ggaacagcgc
      961 ggcgccagtc agcacgatga tcggcccggt gggcaggtcg gcgaatttcg cgctcagcag
     1021 cgtgccgacg aaacccatgc tgaagccaaa accgcctgcc aataacagca gcgtgcccag
     1081 ccggtcggtc cagaagcggg ccgccgcgcc gggcatgatc agcaaggccg aaacgaggat
     1141 cacgcccacg ctgggcaggc cgatcaccac ggccgccgcg accagcgaca tcagcaagag
     1201 atcgacgccg tacaccggcc agccgagcga ctgggcgaac tcggcgtcga acgagactag
     1261 ctgaaattcc ttgtagaaca ccacgatcag cgccaggcag agcgcggcgg cggccaggat
     1321 cacgtacacg tccccctgcg tcatgccggc cgtcttgccg aagatgtacg agttgaggcc
     1381 ggcccggctg ccgacgcccg gcaggttctg gatgatcttc agcagcacga cgcccaggcc
     1441 gaagaagacg gtcagcacgg tgccgatggc ggcgtcgttc ttgacgcgcg tccagcggcc
     1501 gagggccgag atgatcgcga tgccgacgac gccgctggcc agtgcgccca ggagtagcgc
     1561 gatcaggtcc cgctcgcgga cgatcaggaa ggccagacac aatccgggca gggccgcgtg
     1621 cgagagggcg tcgcccgtga gggcccgctt ccgcaggacg gcgaaggccc cgatgatccc
     1681 cgcgccaatt cccagcagac tggtccccgc caacacgacc agcaggttgt agttcaaacg
     1741 agcatattgg agcagccagt cgatcacagg gctcgcgtct ccgcttcgac ggcctcgctg
     1801 gccgcgtcga gcaccgccag ccgcccgccg taggtcttcc gcagattctc gggcgtgaac
     1861 accagctccc gcggcccggc ggccaccagc cgcacgttga gcagcacgac gtagtcgaag
     1921 tactgcggga cggtccgcag gtcgtgatgc acggcgatga tcgttttgcc ctgctggcgg
     1981 aggtcctcca gcacctgaaa gatcattcgc tcggtcgccg catcgacgcc ggccatcggc
     2041 tcgtccatga aatagatatc cgccttctgg gccaaggcgc gggccaggaa gacgcgctgc
     2101 tgctgaccgc cggaaagctg gccgatctgc tgccgttcgt agctcaccag gccgacctta
     2161 tccaggcact tccgggccca gtcgcgctcg gccgcgcccg ggcggcgaat ccagccgagc
     2221 tttccgtagg tccccatcag cacgacgtcg agcacgctca ccggaaaatc ccaatcgacg
     2281 ctttcccgct gcggcacgta cccgatccgc ttgcgaaccc tggccaccgg ctgcccgaag
     2341 acgtggacgc tgccgctggc catcggcacc agccccagca ccgctttgat cagcgtgctc
     2401 ttgcccgcgc cgttggggcc gacgatcgcc gccagttgcg gctgcttcaa ggtcagatcg
     2461 acatcccaca gcaccggccg gcggtgatag gcgacggtga cgtcgtgaat gtcgagaatc
     2521 gtggtcacga tcaaattcca agaaccaaag aaccaaagaa ccaaaagagc aggaacctcc
     2581 gcattggttc ttcggttctt cggttctttg gttctttgat tttcacttca gcgccttgac
     2641 gatcgtatcc acattatgcc tcaccattcc ctcatacgtc ccgtcgggcg tgccttcgtc
     2701 gcccatggcg tccgagaaga gcgtgccgcc gatgacgacg gtgtggccgc gggcggcggc
     2761 gccttcgagc agcgagcgga tgttctggtc cgagacgctg gtctcgacga agaccgcctt
     2821 gatcttccgc tcgacgagaa aatcgaccag ctcgcgaatc cgccgcacgc cggcctcgct
     2881 agtcgtgctg atgccctgga tgccgcggac ttcggtttcg taggcgttgc cgaaatagtg
     2941 gaaggcgtcg tgcgctgtga ccagcacgcg ctgctccttc ggcaccgacc gcatttgctc
     3001 gcccgcatag gcgtgcagtt cggccaggcg agcctggtac gccttcaggt ttgcgcgata
     3061 ggtctcggcg tgcggcggat caaacgcgat cagcgcatcg gccgctgact cggccgccac
     3121 gctccacagg gagacatcga accagacgtg cggatcgtgc gcgccgcccg gatcgctgat
     3181 gatcttctcg tgcggaatct tctccgccac cgccgtggtc ggcttgcgct gccccatgcg
     3241 gcccagcaat tccgccagct tgccctccag gtgcagaccc gaatagagaa tgatgtcggc
     3301 cttgctgagc tggctcacgt ccgccggcga ggccgtgtag agatgcggat cgacgccggc
     3361 gcccatcagc gccgtcaccg caacgtgctc cccgccgata ttcttggcca ggtcggcgac
     3421 catgcccgtc gtgcaaacga cttgaatcgg gtatttgccc gagaactgct tgggcgctgc
     3481 gacttgcgtg gtggcgccgc tcgaatcgca gcccgcgatg acggccgaca ctgctaacgc
     3541 cgccagcaaa caggaagagt acgtgcgcat aaatggagcc gccggccgga aggccgcgaa
     3601 agtttggttt ggcaaaacgt agattttgac tgctcaaaat tattgcccgc ggaaagcggc
     3661 tcgtcaagtg cgaaatccga tggtccctct ccccgtggga gagggttagg gtgagggtct
     3721 atcgttcacg gcgacagcgc ccttcgcgag tcatcagacc ctcaccccgg ccctctccca
     3781 agggagaggg agtagctcgg tccgcgcttg ttttgcgccg aaacatggat tatcagtaag
     3841 gagttctttt caccttcgcc gcgggaggct tggctatgtg tcgcactcgc cgttgtcgcc
     3901 acggtttcac ggtcgtcgag atgctggtgg tcatcgcgat catcgcagtg atggcggggt
     3961 tgcttctgcc ggcggtgcag atggcccgtg aagccgcccg ccgcacccag tgccagaaca
     4021 atctcaagca actggccctg gc
//
LOCUS       DASRJB010000024         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1699565, whole genome shotgun sequence.
ACCESSION   DASRJB010000024 DASRJB010000000
VERSION     DASRJB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699565"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2328
                     /locus_tag="VFB80_00595"
     CDS             <1..>2328
                     /locus_tag="VFB80_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN32274.1"
                     /translation="SLVPVARPPLPAATRHGWCRTPIDSFILARLEKEQLAPSPPADR
                     TTLCRRLYFDLIGLPPSPEEAIALAADETPDAFERLADRLLASPRYGERWARHWLDVV
                     RFAESHGFEMNQPRPNAWPYRDYVIRALNSDLPYDQFVRQQIAGDLLDANEATGFLVG
                     GPWDQVKSPDPVLTAQQRADELHDMIHTTGTTFLGLTVGCARCHSHKFDPIPQTDYYA
                     LKAVFAGVQHGERPRDAGEARQRSQQAEKVRQELAAVVGELDQFEPAADVHAPYETLR
                     APVSRGRNVDLFAPAEARFVRFTITATTDIEPCIDELEVFTAGPQPRNVALASAGTKA
                     TSSGNYPHSDIHKLEHLSDGQYGNSRSWISNERGKGWVQLEFAEPHSIDHVIWSRDRS
                     DPPRFADRVAKEYLIEVSLDGQQWQAVASSADRQPLVDAARSEIVRRQLSQEDKGRLD
                     DILAREKDLETRIRELTTVPAVYAGKFTEPEKTFRFHRGDPTQPREAIEPGTLTQIGT
                     PAALSAELSEQQRRQALADWIVAQENPLSARVIVNRLWQWHFGEGIVSTPSDFGVNGA
                     RPTHPELLDWLAAELVAGNWELKRIQRRIVASAVYGQAPVHGPQSTVHGRDSRLAASV
                     DSANRLLWHFPARRLEAEPLRDAILAVSGNLNLRMGGPGFDLFEPNTNYVKVYTSRKD
                     FGTDQWRRMVYQTKPRMQLDDTFGAFDCPDAGQVAPKRTRSTTPLQALNLLNSAFMVQ
                     QSEIFAKRIDAEIGSDVERQVQRAFALALQRQPAA"
BASE COUNT          441 a          760 c          744 g          383 t
ORIGIN      
        1 ggtcgctagt gccggtcgcc aggccgccgc tgccagcggc gacgcgccac ggatggtgcc
       61 gcacgcccat cgacagtttc attcttgcac gtctggagaa ggagcaactc gcgccgtcgc
      121 cgcccgccga cagaaccacg ctctgccgcc ggctctactt cgacctcatc ggcctgccgc
      181 cgtcacctga ggaagccatc gcactggcgg cggatgaaac accggatgcc ttcgagcgtc
      241 tggcggaccg gctgctcgct tcgccgcggt atggcgagcg gtgggcccgg cactggctgg
      301 atgtcgtgcg ttttgccgag agccacggct ttgaaatgaa ccagccgcgt ccgaacgcct
      361 ggccgtaccg cgactacgtc atccgcgcgc tgaacagcga cttgccctat gatcagttcg
      421 tgcggcagca gattgccggc gatctactgg acgctaatga agcgactggc tttctcgtcg
      481 gcggaccatg ggatcaggtg aagagtcccg atccggtgct cacggcacag cagcgggccg
      541 acgagttgca cgacatgatc cacacgacgg ggacgacgtt ccttgggctg accgtcggct
      601 gcgcccgctg tcatagccac aaattcgatc caatcccgca gaccgattat tacgcgctca
      661 aggcggtctt tgcgggagtg cagcacggcg agcggccgcg cgacgccggc gaagctcgtc
      721 agcgatcgca gcaagcggaa aaagtgcggc aagaattggc ggccgtcgtg ggcgaacttg
      781 accagttcga gccagcggct gatgtgcacg ctccctatga gacgctgcga gcgccggtca
      841 gccgcggcag aaacgtcgac ctttttgcgc cagccgaggc gcggtttgtg cgattcacca
      901 tcaccgccac aaccgacatc gaaccgtgca tcgatgagct ggaggtattc acggccggtc
      961 cgcagccgcg aaacgtcgcc ctggcatcgg ccggtacgaa ggcgaccagc tccgggaact
     1021 atccgcacag cgacatccac aagctcgaac atctaagcga cgggcagtac ggcaacagcc
     1081 ggagctggat ttccaatgag cgcggcaaag gctgggtgca gctcgaattt gccgagccgc
     1141 attcgatcga ccacgtgatc tggagccgcg accggagcga tccgccgcga tttgctgatc
     1201 gcgtggccaa ggagtatctc atcgaggttt cattggatgg ccagcagtgg caagcggttg
     1261 cctcgagcgc ggatcggcag ccgctggtcg atgccgccag gtcggaaatt gttcgccgcc
     1321 agctttccca agaggacaag gggcggctgg acgacattct ggcccgcgag aaggatctgg
     1381 aaacgcgaat ccgagaattg acgacggtgc ccgccgtcta tgccggcaaa ttcacggagc
     1441 cagagaagac ctttcgcttc caccgcggcg atcccacgca gccgcgcgag gcgatcgagc
     1501 cggggacatt gacgcaaatc ggcactccgg cagcgctatc ggccgaactt tccgagcagc
     1561 agcgccggca ggcgctggcg gattggattg tcgctcagga aaacccgctt agcgctcggg
     1621 tcatcgtcaa ccgactctgg cagtggcact ttggcgaagg gattgtcagc acgccgagcg
     1681 acttcggcgt caacggcgcg cggccgacgc atccagagct tctcgattgg ctggccgctg
     1741 agcttgtcgc cggcaattgg gagctcaaac gcattcagcg gcggatcgtg gcctcggcgg
     1801 tgtacggaca ggcaccagtc cacggtcccc agtccacagt ccacggtcgg gactcgcgac
     1861 tggccgcgtc agtcgactcc gccaaccgcc tcctctggca cttccccgcc cgccgcctgg
     1921 aggccgagcc cctgcgcgac gcgattctcg cggtcagcgg caatctcaat ttgcgtatgg
     1981 gcgggcccgg ctttgacctg ttcgagccca acacgaacta cgtcaaagtc tatacgagcc
     2041 gcaaggattt tgggacggac cagtggcggc gaatggtgta ccagaccaag ccgcggatgc
     2101 agctcgacga cacgttcggc gccttcgact gccccgacgc gggccaggtc gctcccaagc
     2161 gcacccgttc gacaacgccg ctccaggcgc tcaacctgct caacagcgcg ttcatggtcc
     2221 agcagtcgga gatttttgcc aaacggatcg acgcggagat cggcagcgac gtcgaacgtc
     2281 aggtgcagcg agcattcgcg ctggcgctac agcgccagcc ggcggccg
//
LOCUS       DASRJB010000025         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1418311, whole genome shotgun sequence.
ACCESSION   DASRJB010000025 DASRJB010000000
VERSION     DASRJB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418311"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFB80_00600"
     CDS             <1..1152
                     /locus_tag="VFB80_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN32275.1"
                     /translation="QDRLANRDDFQNRRDDIREDWHDRYDDFYDRHDDWHHGCWEGNA
                     GDWWHHMWDEHTALMAFGTTMWGLNRLAYGFGYWGYSNPYYSEPYPIDSGVVLDYSEP
                     IAVETAPATPTEQPAGTPTPGMSDFDAARQAFYQGDYAAALAASNKALAALPNDPIIH
                     EFRSLVLFAQGKYKEAAAGLYSVLSVGPGWDWTTLSSLYPSVEVYTKQLRALEAAVRQ
                     QPQAADARFVLAYQYLTMGDKNSAAMMLQVLYKQTPQDHLIQELLLMTAGPEALGAPS
                     TPAPNTAPAAAVPAMELVGTWSASGQGGTKFDLSLSQDGNFTWSFAQKGKTETVKGVY
                     AVDGNVLAMEPESGGTMLAEVTRPQAGSFSFALLGAPPGDPGLKFVKSR"
     gene            1170..2465
                     /locus_tag="VFB80_00605"
     CDS             1170..2465
                     /locus_tag="VFB80_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN32276.1"
                     /translation="MNTIQVRWLACGGLAGALILFAGSGCGSAAPASSSLPVPKVTVM
                     PVVSQETLDADEYIGKTEASEIVEVRSRVFGFLKTIEFQDGDSVTEGKVLFTIEPDEY
                     KAIHQQSVSRIALNDATLALAKAKHARNEVLVKTNAVTREEYEESLAAVKSSEAAISA
                     ARADADRTAVDLKYTEIKAPINGRIDRAMVSKGNLLTGGTGSGTLLTKIVNEQPMYVY
                     FDVDERSLLRYMRMRSESRTAPGSLRDAGIACSLQLADEKDFPHTGKLDFVETEVNPS
                     TGTARLRGVFANESRELASGLFVRVRIPVSKPYQAMLIPEQALATDQSIKFVYVVGDD
                     GTAKRRNVELGPERGEMRIITSGLQAGERVIVKGLQRVKPDQKVEAETAQASLPPSTM
                     PPAKAPTTVRKPVTPAPATKGNPGKASPANTEPRNSQER"
     gene            2472..>3478
                     /locus_tag="VFB80_00610"
     CDS             2472..>3478
                     /locus_tag="VFB80_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN32277.1"
                     /translation="MAKFFIDRPIFAAVISVIITLAGGIAVFVLSVAQYPEITPPTVQ
                     VSCTYPGASSRVVAETVAAPIEQQVVGVENMLYMSSQSTNDGGYNLTVTFEVGTNLDM
                     AQVLVQNRVNLALPSLPPEVKQTGVSVKKKSPNILLVVNLISPGGSYDQLYLSNYATI
                     QIRDELAQIKGVGDVTYLGQLDYSMRVWLDPEKMAARDLSASDIANALREQNVQVAAG
                     ALGRPPVPQGQAFQYTLSTLGRLTGPEEFGSIVVKSGDEGQLTRLRDVVTDVRTDEHG
                     RKFGGIQLGAKVEDTSCSLDGEPSIGLGIFQQPGSNALKTAEAIGTRMEELKRGFPPD
                     L"
BASE COUNT          669 a         1124 c         1152 g          533 t
ORIGIN      
        1 caggatcggc tggccaaccg cgacgatttt cagaaccgcc gagacgacat tcgcgaagac
       61 tggcacgacc gctacgacga cttctacgac cgccacgatg actggcatca cggctgctgg
      121 gagggcaacg ccggcgactg gtggcaccac atgtgggacg agcacaccgc gctaatggcc
      181 tttggcacga cgatgtgggg cctcaaccgc ctggcctacg gcttcggcta ctggggctac
      241 agcaatccgt actacagcga gccctatccg atcgacagcg gcgtagtgct cgattattcg
      301 gagccgatcg cggtcgagac cgccccggcc acgccgaccg agcagccagc cggcacgccg
      361 acgcctggaa tgtccgattt cgacgccgcc cggcaagcct tctaccaggg cgactacgcc
      421 gcggcgctgg cggcgagcaa caaggccctc gctgcgctgc cgaacgatcc gatcatccac
      481 gagttccgct cgctggtgct gttcgcccag ggcaagtaca aggaagcggc agcaggcctc
      541 tacagcgtgc tgtcggtggg ccccggctgg gattggacga ccctcagcag cctgtatccc
      601 agcgtcgaag tttacacgaa gcagctgcgg gccctggaag ccgcggtcag gcagcagccg
      661 caagcggccg acgcgcggtt cgtgctggcc taccagtatc tcacgatggg agacaagaat
      721 tcggcggcga tgatgctgca agtgctgtat aaacagacgc cgcaggatca cctgatccag
      781 gagctgctcc tgatgacggc gggcccggag gccttgggcg cgcccagcac gccggccccg
      841 aatacagccc cggcggcggc agttccggcg atggagctcg tgggaacgtg gagcgcctcg
      901 gggcaaggcg gtacaaagtt cgacctgagc ctgtcgcagg acggaaactt tacctggagc
      961 tttgctcaga agggcaagac cgagacggtc aagggcgtgt atgccgtgga cggcaatgtg
     1021 ctggcgatgg aaccggaaag cggcggcacg atgctggcgg aggtgacccg gccgcaagcc
     1081 ggcagcttca gctttgcgct gctcggtgcg ccgcccggcg atccggggct gaaattcgtc
     1141 aaaagccgct gatttgcgga ggactttcca tgaatacgat tcaggttcgc tggctggcgt
     1201 gcggaggctt ggcgggcgcg ttgatcctgt tcgccggctc cggttgcggc agcgcggcgc
     1261 cggcgtcgtc gtcgctgccc gttcccaagg tcaccgtgat gcccgtcgtt tcgcaggaga
     1321 ccctggatgc cgacgagtat atcggcaaga ccgaagcttc cgagatcgtc gaggtccgct
     1381 ctcgcgtctt tggcttcctc aagacgatcg aatttcagga cggcgactcc gtcaccgaag
     1441 gcaaggtgct gttcacgatc gaaccggacg aatacaaggc gatccatcag cagtcggtat
     1501 cgcgaatagc cctgaacgac gccactctcg ccttggccaa ggccaaacat gcccgcaacg
     1561 aagtgctggt caagaccaac gccgtcaccc gggaggaata tgaggagtcg ctggcggccg
     1621 tgaagtcgtc ggaagcggcg atctccgcag cccgggccga cgccgaccgc accgcggtcg
     1681 atctgaagta caccgagatc aaggccccga tcaacggccg catcgaccgg gccatggtca
     1741 gcaagggcaa cctgctcacc ggcgggaccg gctcgggcac gctgctgacg aagatcgtca
     1801 acgagcagcc gatgtatgtc tatttcgacg ttgatgagcg gtcgctgctg cggtacatgc
     1861 gaatgaggtc cgagtcgcga accgcgccgg gcagcttgcg ggatgcgggg attgcctgct
     1921 cgctgcagct ggccgacgag aaggacttcc cgcacactgg gaagctcgac ttcgtcgaga
     1981 cggaggttaa tcccagcacc ggcaccgccc ggctgcgagg cgtgtttgcc aacgagagcc
     2041 gggagttggc cagcggcctg ttcgtgcggg tccgcatacc cgtcagcaag ccctaccagg
     2101 cgatgctcat tcccgagcag gccctggcga ccgaccagag catcaagttt gtgtatgtcg
     2161 tcggcgacga cggcacggcc aagcggcgaa acgtcgagct gggcccggag cggggtgaaa
     2221 tgcggatcat cacgtccggc ctgcaagcgg gcgagcgcgt gattgtgaag ggcctgcagc
     2281 gcgtgaagcc cgaccagaag gtcgaggccg agacggccca ggccagtttg cctccttcga
     2341 ccatgccgcc cgcgaaagcg ccgacgaccg tccgcaagcc agtgacaccg gcgccagcga
     2401 ccaagggaaa tccagggaag gcttcgccgg cgaataccga gccgcgaaac tcgcaagaac
     2461 gctgaaaaca cgtggccaag tttttcatcg accgcccgat cttcgcggcg gtcatctcgg
     2521 tgatcatcac gctcgccggc gggatcgccg tgttcgtgct ctcggtggcg cagtatcccg
     2581 agatcacgcc gccgacggtg caggtcagct gcacctatcc cggggcgagc tcgcgggtcg
     2641 tcgccgagac cgtcgccgct ccgatcgagc agcaggtagt cggcgtcgag aacatgctct
     2701 acatgagctc gcagagcacg aatgacggcg gctacaactt gacggtcacg ttcgaggtcg
     2761 ggacgaacct cgacatggcc caggtgctcg tgcagaaccg cgtcaacctg gcgctgcctt
     2821 cgctgccccc ggaagtaaag cagaccggcg tgagcgtgaa gaagaagtcg ccgaacattc
     2881 tgctcgtcgt caacctgatt tcgcccggcg gcagctacga ccagctctac ctgagcaact
     2941 acgcgacgat ccagattcgc gacgagctgg cccagatcaa gggcgtcggt gacgtgacct
     3001 acctcgggca gctcgactac agcatgcggg tctggctcga ccccgagaag atggccgccc
     3061 gcgacctgtc ggccagcgac atcgccaacg ccctccgcga gcagaacgtg caggtcgccg
     3121 ccggcgccct cggccgtccg ccggtgccgc aggggcaggc ctttcaatac acgctcagca
     3181 cgctcggccg cctgaccggc ccggaggagt tcggctcgat cgtcgtgaag tccggcgacg
     3241 aaggccagct gacgcggctg cgggacgtcg tgaccgacgt ccgcaccgac gagcacgggc
     3301 ggaagttcgg cggcatccag ctcggcgcga aagtcgaaga cacgtcgtgc agcctcgacg
     3361 gcgagccctc gatcggcctg ggcatcttcc agcagccggg cagcaacgcc ctgaagaccg
     3421 ccgaggcgat cggcacgcgg atggaagagc tgaagcgcgg ctttccgccg gacctgga
//
LOCUS       DASRJB010000026        28376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_290091, whole genome shotgun sequence.
ACCESSION   DASRJB010000026 DASRJB010000000
VERSION     DASRJB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 28376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28376
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290091"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..974
                     /locus_tag="VFB80_00615"
     CDS             147..974
                     /locus_tag="VFB80_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN32278.1"
                     /translation="MHSDQPNALTAARALAWREIVRFFRQRSRIVGSIGTPLVFWLLF
                     GAGLSRSFRIGASGSEQSFLTYFFPGSLLLIVMFTAIFSSISIIEDRREGFLQGVLVA
                     PIPRWAMILGKVLGGTFVAVVQGLIFLLLGLTLPIDLSLVGAAQLVLLLAVAAAALTS
                     LGTFFAWKTDSSQGFHAVMNLVLMPLWLLSGGFFPAPAVAADASWSAWLLHAAMRANP
                     LSYAVGGVRRLLAPAAEYPGAWVPGLAASWLVTIVFAAVAFGLAVWISRERTAGDLQ"
     gene            971..1669
                     /locus_tag="VFB80_00620"
     CDS             971..1669
                     /locus_tag="VFB80_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZN32279.1"
                     /translation="MNRTIFYTLLAAFLASGALATVMGYRVIAGMHDGGSRSGTAREP
                     DPEYQRLPPDGATDWMTGFTLVERSGKEVQWKDFEGKVRVTNFFFASCPGTCLQQNRN
                     VQNIQQGYIGKDVVFLSITCDPDNDSPQRLREYAENLGADRNQWLFLTGRASYIERLG
                     QEMFDVSLGRSKEGPTHSERLIVADKWGNVRGAFAWNDYAQMTQLRMLVDQLLAETEP
                     PAKFRRAPEASASP"
     gene            1666..2124
                     /locus_tag="VFB80_00625"
     CDS             1666..2124
                     /locus_tag="VFB80_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZN32280.1"
                     /translation="MTELAPLLPHLNAALNALATVLLVAGFILIKQRREAAHKWTMLA
                     CFAVSILFLASYLTHHWYLKYVVKLPYGSHLFPAEYPVARAIYLGVLIPHVILAAAVP
                     FLAMGSIALGLADWRKAHRRLSWWTFPIWLFVSISGVIVYVMRYQLYPPH"
     gene            2146..2409
                     /locus_tag="VFB80_00630"
     CDS             2146..2409
                     /locus_tag="VFB80_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32281.1"
                     /translation="MKPAALLQTFALGLLLLALCSGAALGCPTCKDALSHDPATANLA
                     RGYAYSILFMLSMPPLIFGSLCAYFYWEVRRARARQTAVPSNS"
     gene            complement(2406..2771)
                     /locus_tag="VFB80_00635"
     CDS             complement(2406..2771)
                     /locus_tag="VFB80_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HZN32282.1"
                     /translation="MRFFTLLGRALALRCPLCGRGRLFRGWFAMHEKCPECGASLERE
                     PGFFLGSIYINYGLTGLIAVVAALVLRFRYGVPQNPLLVGGMAFTVVFPLLVFPWARS
                     LWLGFDQWRDPREGEIGTR"
     gene            2968..3447
                     /locus_tag="VFB80_00640"
     CDS             2968..3447
                     /locus_tag="VFB80_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32283.1"
                     /translation="MNSNRRAFLFIDREVQGALLLRTASYWLFCLISISLMVLCWNAL
                     TGPPRRFVDLTTEFYFRYGPALVASLIMLPIVMFDVLRMSNRFVGPVVRLRTGLNELA
                     EGRPAQPLNFRDDDFWRGLANEFNRAAARLAREGTELSVPTEEMSATEVERASSLSS"
     gene            complement(3457..4221)
                     /locus_tag="VFB80_00645"
     CDS             complement(3457..4221)
                     /locus_tag="VFB80_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32284.1"
                     /translation="MKEGRSRSSRYHSYCDDYYVNMNLNTEMELPQSRETVLHFFEQV
                     KKTYPGMKNFYSRERGEFVLEEDKDKGDYRWASIENRRVSSGYVNPPTVEEALKQHSL
                     VLDKIPYMLSVSPLDCESLNVMYGFDYTYRGNHNQLLVDALGVQPAFEKMTEIPGASI
                     LSNEPSIQFALDEECRTQCRLSIEARTSAYHVRMKEFPEEQLSVYFTARRYGSLDGEE
                     SFVEAMRKLADICREVIDGYVVENVLRPLQQTIAIS"
     gene            4354..4920
                     /locus_tag="VFB80_00650"
     CDS             4354..4920
                     /locus_tag="VFB80_00650"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN32285.1"
                     /translation="MSKATPQSHVFPSDLGWMALCWSGERLCRFTFGHPSAAAAAASL
                     AADEADAERADPPKWVAELAGRLQSFAAGRAELFDDVPLDLSHLSAFQGRVVKACRKI
                     GRGKVRTYGELAQLAGAPGAARAVGSVMARNRFPIIVPCHRVVGAGGSLGGFSARDGV
                     NLKRRMLALEGAVTGKPAQRSRFHASHA"
     gene            complement(4887..5621)
                     /locus_tag="VFB80_00655"
     CDS             complement(4887..5621)
                     /locus_tag="VFB80_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HZN32286.1"
                     /translation="MHPSPMLLLDLTLPTAAENVALDEALLDAAEAGEIDDEILRLWE
                     APAPLVVVGRSSRVTAEVNLDACRVAHVPVLRRSSGGAAIVAGPGCLMYAVVLRYAGR
                     EHLRLLDEAHRHVLSALAAALSTLVANVQPLGTSDLALGWRKFSGNSVRCKRDHLLYH
                     GTLLYDFDLTVVERLLLMPPREPEYRRGRQHGEFLTNLGIPAQVLRSVLARGFAAHKP
                     LVDWPRERTAGLVRERYACDAWNLER"
     gene            5789..6943
                     /locus_tag="VFB80_00660"
     CDS             5789..6943
                     /locus_tag="VFB80_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN32287.1"
                     /translation="MSLSSAAPTPPEGPRLPAAASPVHPSLPPVEAPSGTFILQLFLI
                     PLLIVSIVVVLWLMFSWMASMGRDDPAKLAESIQWGDEASWQRSYELADLLRSPDQKY
                     DSLRRDATTAAKLASFLNRDLNEPLEGTVKENQQRVMRRMYLCRALGSFSVPTALDVL
                     LRAATQEGKPVEIEVRYSAIEAIATLADNCGPEFMDDPALPKKVLPVLLAASEAQESG
                     EEIAAAPPVPEKDGKRTIYRPHAELRAVAAYALGVVGGEEATNRLKAMLYDTYPNARY
                     NAATGLARAGDAECERVLKEMLDPENEVAIRDERYKDDQARKRTTVLLNGVKGTLILA
                     ERNPQADLSRLKESLVRLSKSPLENIQIERNKVKRAASEALERLDPSAKR"
     gene            6948..9200
                     /locus_tag="VFB80_00665"
     CDS             6948..9200
                     /locus_tag="VFB80_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN32288.1"
                     /translation="MKRIKSGGGWRAILYSLQKSRQMGGLWGLWKAMRSKNACKTCAL
                     GMGGQKGGMVNEAGSFPEVCKKSLQAMAADMQGAIKPEFWSTYSVPQLQAFSPRELES
                     CGRLVQPVLLENGSQYYRPIEWDDALGRIVAKLTGTAPDETFWYFSGRSSNEAGFLLQ
                     LFARLYGTNNVNNCSYFCHQASGVGLSSSLGTGTATVTLEDVEHADLVFLIGGNPASN
                     HPRLMTTLKNVRRRGEVIVINPVIETGLVNFRVPSDPWSLLFGTKIATLYVQPHIGGD
                     LALMTGIAKRIDEMGAQDEAFLQEHCRHAEQWLAHLRQIPWAGIHAKSGVEQAEIDQI
                     AERYAAAKDVVFSWTMGITHHAHGVANVQAIANLALMRGMVGRPRAGLMPIRGHSNVQ
                     GIGSVGVTPQLKQAVFDALERELAVKLPTTPGLDTLGCIEEAHAGRLKAGFCLGGNLY
                     GSNPDAAFAGEALGRLDQIVYLNTTLNIGHAHGLARETIILPVLARDEEPQPTTQESM
                     FNYIRLSDGGPRRHAGPRSEVEIIAEMGERVLDSSPGYPPGGSAPRDADLTRSARATI
                     DWASMRSTGKIRQAIAKVVPGFAQLAQIDRTKQEFHIPGRTFHQPKFATADGKAILHL
                     HELPELAGGENTLRLMTVRSEGQFNTVVYEDEDLYRGQERRDVILLHPEDLRRLNLTE
                     NQRVTVSSAAGELRGILARSFPEIRAGNALMYYPEANVLVPRHADPHSRTPAFKSIVV
                     SVTAEIPVTV"
     gene            9346..11988
                     /locus_tag="VFB80_00670"
     CDS             9346..11988
                     /locus_tag="VFB80_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN32289.1"
                     /translation="MRHIHFLLLAAIITAGGTAARGQSPAESPRFSRHVVAVFSRLGC
                     NSGSCHGAVQGKNGFRLSLFGARPEDDYDQIVRQASGRRVNLLDVEQSLLLLKATGRL
                     PHGGGRRMEAGSLEYRVLRNWLAAGAVADDVGASQLVSLEVTPREQAARAVEKYELRL
                     AAKFADGSTEDVTALASFASLDAGVATVDAVGRAQAQGVGDTALVARFRADPAVSLVV
                     VAREGGGQLALPDPPSAIDGHILAKLRRLNLPPAELADDATFLRRARLDVTGQLPTAE
                     EVREFLADTSPDKRERKIDQLLAEPGHAALWTLKFCDLLKATDFGVYADGLDEQHEAP
                     RFQAWIRARLAENTPYDEFAARILTATSREGRSLEEWSAEVVALAEGYATPRKDLDLY
                     SRRQTLDAYWQRKDAVGVPGTLQVAHAFLGLRLECAQCHRHPHDVWQQDDLLSFANFF
                     MGVRAVGFQGENDKRYPDESQLAKGFEQEGKKLAEEVKQLKEGRGKELAEKAKQAQPE
                     INRLKNEIQKGGEAAALSEMQQQLDSLQTTIAENDQLQREIREKDQRSKLLTDAIPKR
                     IVHSQIMHQAGDEKSPRFAKVTSPLGTQTSQTFRLPGEREPVALGEGEDPRAKVVEWL
                     RRPDNPFFAKAIVNRVWAHYFGRGIVNPPDDLSPHNPATHPALLDDLWREFVAGGFDL
                     RRLHRAILCSRTYQQCSLPAAASAADRANYACFYPRRLPAEVLLDVLNQATGTRENLD
                     MRYDHWPDGMSTVEIPYKPRNGFVTFMLEQFGRPPRNSAVQCDCARQGDASLLQVLSL
                     ANHPRVRQKVADPSGRVASLIRETEDPQQRIEQLYLSVLSRLPDQLECSACCEHLATT
                     ATPEEGLQAVLWSLLNTREFILQH"
     gene            12003..13352
                     /locus_tag="VFB80_00675"
     CDS             12003..13352
                     /locus_tag="VFB80_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32290.1"
                     /translation="MIGCQTFRRHSRRDFLRIGGASLCGVSLLDMLRAQSQAAGAPAA
                     KAKSMIVCWMAGGPPHTDMFDMKPDSPTDYRGEFKPIKSKLSGLDVCELMPKLAERAD
                     KFTIIRSITTMNQPGDHSRAPMYWLTGNPRLPSGTAEYPMYGSAAAKFRPGPAELPTF
                     AVLGKIDHHIGNSIAGSFLGPAFNPFIFDPLEKKDDIAKMLAPQLELPAFTKNAELLK
                     ALDKRLRRQDGVDPLIAGLDAYQQTAFDMLRSPKLRAALDLSEESDETVARYKDHHLS
                     KYRYPSGDPLHFLLARRLVEAGVPIVHFSLGYWDWHGENFVAGRQQIPMFDAGLSSLL
                     DDLDERGLLDTTIVLALGEMGRKPKCGTEKNAGRDHWDYAQFVLAAGGGFKRGCIVGA
                     TDKLGEQVTDNFYKIESFGRTLYHLLGIDPDTTVYTPSNRPIRLIAEDAPLVKEALI"
     gene            complement(13368..14729)
                     /locus_tag="VFB80_00680"
     CDS             complement(13368..14729)
                     /locus_tag="VFB80_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN32291.1"
                     /translation="MRFTLLATAVLLSTAPLRATEPSAAAQRGYELLTQKAYLPPDFD
                     QETFDNVWRQWPEPLRSEAEKATADERRRMAFERYGLTPRPDDPTQPLQYVVDRQGWT
                     MNCFACHGGLIPDGKGGTQVWPGAPNSRFALQTLTEETRLVKAELTKPLSRMDVGGVF
                     VPLGSTDGTTNAVMFGVVLMGFRDADLVFHADRPVAKLTNHDMDAPPWWHFRRKKMLY
                     IDGFAEKGHRGLMQFMLVRSNGPEKFRAWEQEFRDVFAYLSSVQPPRYPYRIDQPLAE
                     RGRGVFNRTCAECHGTYGDGEGYPEKLVPIAEVGTDRVRLDALTPAHRDAYGQSWFAE
                     FGKKANKSDPGGYVAPPLDGIWASSPYFHNGSVPTLWHVLHPQERPAVWRRTGDEFDS
                     TRVGWQIETFDRLPSDAVFTGWKKRQHFDTHRFGKSAAGHDFPDALTEDEKRAVLEYL
                     KTL"
     gene            complement(15278..16426)
                     /locus_tag="VFB80_00685"
     CDS             complement(15278..16426)
                     /locus_tag="VFB80_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32292.1"
                     /translation="MLLLGFINIFGQQVGWPDLAVVALLVLLEGLLSIDNALVLGLLA
                     KRLPKSEQGKALWYGLAGAFVFRFLAVLLASLLLQWTFAKFLGGAYLVYIAVRHLFFE
                     SKEKADEKIQLDEHGHPVLVEERTGGPLTDEQEEVEIRGRMPVYMSAERRKQFGLASF
                     WPTVLVIELTDIAFAVDSILAAIAMVGAPPRGHPPNALHPKLWVVIFGGIIGLMLMRV
                     AAKWFIWLLEKFPRFELSAYLLVIVIGLKLLADWGVNSDWSFDRPEVVAQQLEKWGWK
                     KSFENFEKRRQGWAHSYEHWLRTKWPLGLEAQHVAAPEQPVQPAPPPPPGVPAAQPPP
                     AKENELQKLLHIPHVLNFHSWRRPEFILFWGLMFVCFCIGFLPPKRKH"
     gene            complement(16503..20231)
                     /gene="metH"
                     /locus_tag="VFB80_00690"
     CDS             complement(16503..20231)
                     /gene="metH"
                     /locus_tag="VFB80_00690"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZN32293.1"
                     /translation="MPAPVRTKAYEQLEALLAERILVLDGAMGTMIMRLGLDEAAKRG
                     ERFRDHCEGDPDIQLVNFSDLLCLTRPDDITQIHREYLAAGADIVETNTFGASLVGIQ
                     EFRFPREMVRELNFAAVACARQAVDEFNERTPERPRFVAGSIGPTTKQMAISTRVDDP
                     AWRAITFDQMVDSYYDQVAALVEAGVDILLPETFIDTLNLKACLFAIERCFQERGVRL
                     PVMASGTFGDGGGTFVSGQVVEAFWNAVNHVPLLTVGMNCALGPETMRPHIEELSHVA
                     TARVHCYPNAGLPDKVTGQYNLGPAAMARLVGEFIENRWLNIVGGCCGTTPEHIAALA
                     EVAGRVRPHEPAEQPQWLRLSGTLPFTQRPDSNFIMVGERTNVTGSKAFARLIRDGKY
                     EEAVEVARQQVAGGATIIDVNMDDALLDGEAAMTRFLRLIAGDPEVQKVPVMIDSSKW
                     SVIEAGLKCLQGKGIVNSISLKDGEEEFLRRAALVRRYGAAVVVMAFDENGQAVEVAD
                     KVRICERAFRVLTIKVGFPAEDIIFDPNILTVATGIEEHNNYAVNFIEATRLIKERCP
                     GCKISGGVSNISFSFRGNDVVREAMHSAFLYHAIRAGMDMGIVNAGQLALYEEIPKEL
                     LNHCEDVLLNRRPDATDRMLQLAERVKGKGKAAATEDLAWRDAPVEERLKHALIKGID
                     KFIEQDTEEARQQYPRCLSIIEGPLMAGMQVVGDLFGAGKMFLPQVVKSARVMKKAVG
                     YLLPFMEEEKRSAGLAAQKARGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVM
                     VPTEKILDTATQQGVDIVGLSGLITPSLDEMVYVAKEMQRRRMCLPLLIGGATTSAKH
                     TAVKIAPQYEQPTVHVLDASRCVGVVDKLLSPQHKPQLDRENRSLQQQLAESYRQRDV
                     KLVPLAQARARRFQTDWQEVDIPTPAFTGIRTLRDFPLAEIAKCIDWSPFFLTYEMKG
                     KYPRIFDDPAAGPHARALFEEAQQMLRRIIDERLLTAHGVYGFWPANSDGDDIVVQEP
                     VNPKSPIPNLKLHTLRQQWERQGQKDFRALADYVAPLGSGRQDYVGAFAVTTGVGCDE
                     LCARFDARHEIDNSILVKALADRLAEAFAELLHQRARRDWGYGLAERFSNDELIDEQY
                     RGIRPAPGYPSQPDHTEKKTLFDLLGAEEHTGIKLTESYAMWPAASVCGLYFAHPQAR
                     YFAVDRITREQVEDYAHRKGMSLAEAERWLAPNLGYEP"
     gene            20492..21778
                     /locus_tag="VFB80_00695"
     CDS             20492..21778
                     /locus_tag="VFB80_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32294.1"
                     /translation="MNLLNEPSTGEARPRLPVLFHLLDVSRQRPSRPPAEPSLASLSE
                     MAEPAAATAVAPPAADLSLATPAVPIETPTISIPPAAPEAAASKSSEPAKSSPPTPRS
                     EAERRRKAKRPTTEEWFAAHGKYIALVFVLALGATIYFARVNRRPPAVEEPPDMALNI
                     EPGDSARSTANSKPSTTLVGDSVPNPATSTGSPAKLIETASASAPQAELFPPTETQVA
                     ARNPSAAARPAASDLFPDKKSDSRVAVQDRGQDALANSSPTNSASPLASAAPAAPATA
                     SAPGAAPAAATASAMEPSYPHTGFPPTGLPAASYPTTHPPVTNYPPTNIPGHAVPAPP
                     AMPAQPPAWSHSPYQPSPYSPTQFQPPQSPTQFQPPQSPTQFQPAQRPPLGYQPVSPA
                     PGGSFAPPGGAIPPQYQPPFNSVPGTRYERSGPGLY"
     gene            21750..23441
                     /locus_tag="VFB80_00700"
     CDS             21750..23441
                     /locus_tag="VFB80_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32295.1"
                     /translation="MSALDQAFIKAYAKDQPADRAAAVASRGAPPRAVAGARVAAGAI
                     EQVYRDGSLYRVDLPARISPSAAAIPQPHVKLLPPTSPRRSVQRSLLRLLANQPVPPP
                     ADAELPPKVARKVIIRHIAHSIAPAPLGLLRPEVGEPLTLAEVDELPPETIIEWPPAP
                     AVAPPPAPIAAPQTAPLNEAHAIPQLVEERPIAVHGQWALAESAAGLVVVAEPAEFDE
                     AGSDGLVAVRLESLTLPEPPAAEPFAEESLVEAADFELHEAERTRFAGKSLDDADNGE
                     PAFRVDPPHAASIHRPHARFTAEPQPVDELTLAETPPEPQTQAEAAAPVESPAAEPAS
                     ATSVAPLWEVDRFHWPKTCEKLLSAEGSYFVHAGAKLVAAVKDGLRVLAITGTRRGEG
                     RTTLALCLARVAAQAGVQAAIMDADFARPQLASKIGLAIAHGWQDAALGKIPLSEAAV
                     KSLADQVTALPLEAAGAASRLSLADPRVTATIRAAAATFELLILDLGPVGPGEEVAFP
                     PGEKCPLDAAIVVRDMRFATLAESEAIGHGLSDAGVEAVGIAENFVVEEEPQYSS"
     gene            23470..24294
                     /locus_tag="VFB80_00705"
     CDS             23470..24294
                     /locus_tag="VFB80_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32296.1"
                     /translation="MYEAYWQLDARPFEHTLDERFHFAAESQQGAVLKLRYVLENRRG
                     AALLAGESGLGKTLVAQSLLAQLSPAHQPRLHIVYPQMPAEQLLAYLADQLTGQTAPL
                     AAGVDQCVLRIEQALRGGATEGRHAVIVIDEAHLLRQTQALESIRLLTNFQHEGRPLA
                     TFLLVGQTGLAVATERWPQLDERLAVKCILTRLSAAETRGYIEHRLSAAGAKRPIFSE
                     TAFATIHELTQGIPRRINRLCDLALLVGYGEELSILQPEHLESIHEELIGRAATAA"
     gene            complement(24319..27669)
                     /locus_tag="VFB80_00710"
     CDS             complement(24319..27669)
                     /locus_tag="VFB80_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN32297.1"
                     /translation="MDLKRFVAPFSPPTTTLIDSLRYWTEQQPQETAYYYTDGEDQEQ
                     ALTYEQFDRHARAIAARLTDLGLTGQRALLLYPQGLEFVVAWFGCLYAGVIAVPAFPP
                     RRNRNMERIKAIADDCQAQAALTEQDVLGRIEDLLEDAPSLKQLHWLATDRVSDTEGA
                     GWNPPRIDPASLAMLQYTSGSTGTPKGVMLSHANLMHNVQLICHSFEVHRKGSGLSWL
                     PTYHDMGLVGGTLFAMFFGRPAVLMSPMAFLQKPIRWLRGITKYGTTVSGGPNFAYDL
                     CTDKITDDQLAGLDLSAWEVAFNGAEPVRAETLRRFTERFGPYGFRAESHYPCYGMAE
                     TTLIVTGSYANRPKIVRSFDARQLDAKVVVPVAASAAGGRALVGCGHVLPDEHVKIVN
                     PLSRRELRPDKIGEIWVNSPSVAQGYWNNPADTEQTFRAHLAGDGGRAYLRTGDLGFF
                     DQGELFVTGRIKDLIIVRGVNRYPQDIEMTVERANPRIQPQAVGAFAVDLEGRERLII
                     VAEVERTRRDDWSDVIADVRKAVTAEHELPPDAVILVRFGSVPKTSSGKIQRHACREE
                     FLGGSLQIIQQWKAWEAGAEEQPAASAPAESAEGKSLADVDPAIAQIVMDHVRAIAKE
                     RAKVLALDSNIVTDLGLDSLERLQIANTLEETFGGRFPEDVLAEIETVREVAAAIATY
                     IGTEPRVQRHAAAGPTANWASAGEIPPECYDFSQMPEYKRLKQTMAMLGSTGVPNPYF
                     RAHEGATRDIARIDGRELISFATYNYLGMSGDPEIAQAAKVAIDQFGTSTSASRLVSG
                     EKTVHVELEREIARFHGLEDSICYIGGHCTNESTLGHLFGPGDLIVHDSLAHNSIIQG
                     SVLSGARRRPFPHNDWRAVDALLAEVRRDYHRVVIAIEGVYSMDGDFPELPPFVEIKK
                     RHKALLFIDEAHSLGVLGKTGRGLTEHYGVDPRDVDLLMGTLSKAMGSCGGYIAGTKE
                     LIEYLKYTSPGFVFSCGLSPPGAAAALASLHLLQRDPSRIERLQANSRLFLELARQRG
                     LNTGMSNNTAVVPIITGNSYHALLLSNALFDRGVSVQPILYPAVEEEKARLRFFITAA
                     HTEQQIRSTVAAVAEEIEKIDPRYVAGT"
     gene            complement(27796..>28376)
                     /locus_tag="VFB80_00715"
     CDS             complement(27796..>28376)
                     /locus_tag="VFB80_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32298.1"
                     /translation="GYGLVAADLTPVTTAQTAWNTGFAAQQTAQAAAEAATQTKDAQR
                     ESLVAAVRTVVRKIQATPAVTGSAKAAAGITVPDTTPTPSGPPTTAPVGQVDTSQRLQ
                     HTVSFVDSATPTSKAKPAGVRGCEIWMKIGTPPPAAAGDLVFVTLDTRTPHTIPFEGA
                     DGGKLVTYWLRWVSTRGETGPWSAAVGATVPG"
BASE COUNT         4884 a         9378 c         9192 g         4922 t
ORIGIN      
        1 cctgggcaag ccgacgctgg aggacgtatt catcattcgt acgggacatc gatttcagat
       61 ttaggatttc agatttcaga tttacgaacc aaatgaatct cgtgccaatc taaaactgaa
      121 atctgcaatc tgaaatagca ccaccaatgc actccgatca acccaacgcc ctcacggccg
      181 cccgcgccct tgcctggcgc gagatcgtcc ggttcttccg gcagcgcagc cggatcgttg
      241 gttcgatcgg cactccgctg gtgttttggc tgctgttcgg cgcagggctg tcgcggtcgt
      301 tccggatcgg cgcgagcggc agcgagcaga gttttctcac gtacttcttc ccgggcagcc
      361 tgctgctgat cgtgatgttc accgcgatat tctcgtcgat ttcgatcata gaggaccgcc
      421 gcgagggttt tttgcagggc gtgctcgtcg cgccgatccc gcggtgggcg atgattctgg
      481 gcaaggtgct gggtggaact tttgtcgccg tggtccaggg gttgatcttc ctgctgctgg
      541 gtctaacgtt acctatagac ctgtcgctgg tcggcgctgc gcagctggtg ctgctcctgg
      601 ccgtggcggc ggcggcgctg acgagcttgg gcacgttctt cgcctggaag accgacagct
      661 cgcagggctt tcacgcggtc atgaacctgg tgctgatgcc gctgtggttg ctgtcgggcg
      721 ggttctttcc cgcgcccgct gtggccgctg acgcctcgtg gagtgcgtgg ctgctgcacg
      781 ccgccatgcg ggcgaatccg ctcagctacg ctgtcggcgg cgtccgccgg ctgctggccc
      841 cggcggccga atatcccggc gcgtgggtgc caggacttgc ggcgagctgg ctggtgacga
      901 ttgttttcgc cgcggtggcc ttcgggctgg ccgtgtggat cagccgcgaa cgcaccgcgg
      961 gagacttgca atgaatcgga cgattttcta cacgctgttg gcagcgtttc tggccagcgg
     1021 agcgctggcc acggtgatgg gttaccgcgt gattgcgggg atgcacgacg gcggcagccg
     1081 ctcgggcact gcccgcgagc ccgatccgga ataccagcgg ctgccgcccg acggagcaac
     1141 agactggatg accggcttca cgctggtcga gcgaagcggg aaggaagtgc agtggaaaga
     1201 cttcgagggg aaggtgcgcg tgacgaactt ctttttcgcg tcgtgtcccg gcacctgcct
     1261 gcagcagaac cggaacgtgc agaacatcca gcagggctac attggcaagg acgtggtttt
     1321 tctcagcata acctgcgatc ccgataacga cagccctcag cggctccgcg aatatgcgga
     1381 aaatctcggc gccgaccgca atcagtggct gtttcttacc ggccgcgcga gctacatcga
     1441 gcggttgggg caggagatgt tcgacgtgtc cctcggccgg tcgaaggagg gtccaactca
     1501 ttccgagcgg ctcatcgtgg cggacaaatg gggcaatgtt cgcggcgcgt tcgcctggaa
     1561 cgactatgcc cagatgacgc aactgcgcat gctggtcgat cagctactcg ccgagaccga
     1621 gccgccggca aagttccggc gggctccgga ggcgtcggca tcgccatgac cgaattggct
     1681 ccactcctgc cgcacctcaa cgctgcgctg aacgccctgg cgacggtgct gctggtcgcc
     1741 ggattcatcc tgatcaagca gcgccgcgag gcagcccaca aatggacaat gctcgcctgt
     1801 tttgccgtgt cgattctctt tctggcgagc tatctgacgc atcactggta tctcaagtac
     1861 gtcgtcaagc tgccgtacgg cagccacctc tttccagccg agtacccggt cgcccgggcg
     1921 atctacctcg gcgtgctgat cccgcacgtg atcctggcgg ccgccgtacc ctttctggcg
     1981 atggggagca tcgctctggg cctggccgac tggcggaagg cgcaccgcag gctgagttgg
     2041 tggacgtttc caatctggct cttcgtgtca atcagcggcg tcatcgtcta tgtgatgcgt
     2101 tatcaactct atccaccgca ctgagcggcc gataaaatac ggacaatgaa accagccgcc
     2161 ctgcttcaga cattcgcgct cggcctgctc ttgctggcgc tgtgcagcgg ggcggctctg
     2221 ggctgtccga cttgcaaaga cgcgttgtcg cacgacccgg ccacggcgaa cctggcccgc
     2281 ggctatgcct acagcatcct gttcatgctc tcgatgccgc cgctgatctt cggcagtctc
     2341 tgcgcctatt tctactggga agtccggcgg gcgagggcgc ggcaaacggc ggttccctcg
     2401 aactcctagc gcgtaccgat ctctccttcg cgcggatcgc gccactggtc gaagccgagc
     2461 cacaggctgc gggcccaagg gaagaccagc agcggaaaga ccaccgtgaa tgccattccg
     2521 ccgacgagca gcggattctg cggcacgccg tagcggaacc gcagcaccag cgccgccacg
     2581 acggcgatca ggccggtcag tccgtagttg atgtagatcg agcccagaaa aaagcccggc
     2641 tcgcgctcca aggaagcgcc gcactcggga catttctcgt gcatggcgaa ccagccacgg
     2701 aatagccggc cccgcccgca tagtgggcag cgcagcgcga gcgccctgcc cagcaaggtg
     2761 aaaaaacgca tgccctcaat ttaccagcac agcgccaccg ttaatcacgc ccaccgttag
     2821 cgggggcgga gctctctggt gacggccgga gatttgcgcc agcccataca accggacggc
     2881 ccgcctcggt gccatttccg cgcgcaattt atgagctact ttgtcgtagt ggatcgcccg
     2941 ccagtcagat tccagggagc acctgcgatg aattccaacc gccgcgcctt cctcttcatc
     3001 gaccgcgagg tccaaggggc gctcctgttg cggacggcca gttactggct gttctgcttg
     3061 atttccatca gcctgatggt gctttgctgg aacgcactga ctgggccgcc gcggcggttc
     3121 gtggacctga cgaccgagtt ttatttccgc tacggcccgg cgctcgtcgc ctcgctgatc
     3181 atgctgccga tcgtgatgtt cgacgtgctg cgtatgagca atcgcttcgt cggtccggtc
     3241 gtgcgcctgc ggacgggcct gaacgagctg gccgagggcc gcccagccca gccgctgaac
     3301 ttccgcgacg acgatttctg gcgcgggctg gccaacgaat tcaaccgggc cgccgcccgg
     3361 ctggcgcgcg agggcaccga gctgagcgtg ccgacggaag agatgtcggc cacggaagtg
     3421 gagcgggcct cgagcttgtc gtcgtagttg accgtactac gagatggcaa tcgtctgctg
     3481 gagcggccgc aggacgttct ccacgacgta gccgtcgatg acctcgcggc aaatatctgc
     3541 cagcttccgc atcgcttcga cgaacgattc ctcgccgtcc aagctgccgt agcggcgggc
     3601 cgtgaaatag acgctgagct gctcctcggg gaattccttc atccgcacgt ggtaggcgct
     3661 ggtgcgggcc tcgatgctca ggcggcactg cgtgcggcat tcctcgtcca gggcgaactg
     3721 tatcgacggc tcgttggaga ggatggacgc gccgggaatc tccgtcatct tctcgaacgc
     3781 cggctgcacg ccgagggcat cgacaaggag ctggttgtgg ttgccgcgat aggtgtagtc
     3841 gaatccgtac atgacgttca gcgactcgca gtcgagcggg ctgactgata gcatgtacgg
     3901 gatcttgtcg aggacgaggg aatgctgctt gagggcctcc tcgaccgtcg gcgggttcac
     3961 gtaccccgac gagacccgcc ggttttcgat gctggcccag cggtaatcgc ccttatcctt
     4021 gtcttcctcc agcacgaact cgccccgctc gcggctgtag aagttcttca ttccgggata
     4081 ggtctttttg acttgctcga aaaagtgcag caccgtctcg cgcgactgcg gcagctccat
     4141 ctccgtattg aggttcatgt tcacgtagta gtcgtcgcag tacgaatgat agcgactcga
     4201 acgtgatcgg ccttccttca tggtcgtgcc tggctccttc tagggcccgc gaacgtttgt
     4261 acaatgtcag tataagcagc tgcgctattt cggtcaaagc agccgccggc cagcggcggc
     4321 ggccacttgt tgagatatcg ggatccgttt tccatgagca aggccacgcc acagtctcac
     4381 gtgttcccca gcgacctggg ctggatggct ctctgctgga gcggcgagcg gctgtgcagg
     4441 ttcacgtttg ggcatccctc ggccgccgcg gctgcggcat cgctcgctgc tgacgaagct
     4501 gatgccgagc gggctgaccc tcccaagtgg gtcgcggaac tcgccggcag gttgcagtcg
     4561 ttcgctgccg gacgcgctga gctcttcgac gacgtgccgc tcgacctctc gcacctctcg
     4621 gcgtttcagg gccgtgtggt gaaggcctgc cgcaagatcg gccgcggcaa ggtccggacg
     4681 tacggcgagt tggctcaact tgccggagct cccggagcgg cgcgggccgt cggcagcgtg
     4741 atggccagaa accgctttcc gatcatcgtg ccttgccacc gcgttgtcgg cgcgggcggt
     4801 tcgctagggg gattctccgc tcgggacggg gtcaatctga aacggcgcat gctcgcgctg
     4861 gaaggcgcgg tcacaggcaa acccgctcag cgctccaggt tccacgcgtc gcacgcatag
     4921 cgctccctta ccaggcccgc ggtccgctcg cggggccaat cgacgagcgg cttgtgcgca
     4981 gcgaagccgc gggccaggac gctgcggagg acctgtgcgg gaatgccaag gttggtaaga
     5041 aactcgccgt gctggcggcc gcggcgatat tccggctcgc ggggcggcat caagagcagt
     5101 cgctccacca ccgtcagatc gaagtcgtaa agcagcgtgc catgatagag caggtggtcg
     5161 cgcttgcagc gaacgctatt ccccgagaat tttcgccaac ccagggccag gtcgctcgtg
     5221 ccgaggggct gcacgttggc gaccagcgtc gacagagcgg ctgccagcgc cgaaagcacg
     5281 tgccggtggg cttcatcgag caaccgcagg tgttcgcggc cggcatagcg gagcaccacc
     5341 gcatacatca ggcagccggg ccccgcaacg atcgcggccc cgccgctgga gcggcgcagg
     5401 acgggcacgt gggcgaccct gcaggcgtcc aaattcactt cggctgtcac gcgggatgag
     5461 cggccgacca ccaccagcgg ggcgggagcc tcccacagtc gcaggatttc atcgtcgatc
     5521 tcaccggctt cggccgcgtc gagcaaagcc tcgtcgagtg cgacgttctc ggccgccgtg
     5581 ggcagcgtca agtcgagcaa tagcatgggc gatgggtgca tgcgagcctc gtgccgaggg
     5641 ggatctcacg aggtggccgg acggcccgcc gaccgcccaa accaagatgg caattcgact
     5701 tacgttgcct tcccgcccac caacgtcccg ttgacgcccg ataccccaaa cttacactga
     5761 attcgtatct gctgcagccg gagttcttat gtcgctttca tccgccgctc ccaccccgcc
     5821 tgagggtccg cggctgcccg ccgcggcatc accagtccat ccgtcgctgc cgccggtcga
     5881 ggcgccgagc ggaacgttca ttttacagct ctttttgatt ccgctcttga tcgtctcgat
     5941 tgtggtcgtg ctgtggctga tgttcagctg gatggccagc atgggccgcg atgatccggc
     6001 aaagctggcc gagtcgatcc agtgggggga cgaggccagc tggcagcggt cgtatgaact
     6061 ggccgacttg ctccgcagcc ccgaccagaa gtacgacagc ttgcgccgcg atgcgaccac
     6121 ggcggccaag ctggccagtt tcctgaaccg cgacctgaac gagccgctcg aaggtacggt
     6181 caaggagaac cagcagcgcg tgatgcggcg gatgtacctc tgccgggccc tggggtcatt
     6241 cagcgtgccg acggcgctcg atgtgctgtt gcgggcggcg acgcaagaag gcaagccggt
     6301 cgaaatcgaa gtccgctaca gcgcaatcga ggcgattgcg actctggccg acaactgcgg
     6361 cccggaattc atggacgatc ccgctctgcc gaaaaaggtg ctgccagtgc tcctggccgc
     6421 ttccgaggcg caggagagcg gcgaggagat cgctgcggct ccgccagttc cggaaaagga
     6481 cggcaagcgc acgatctatc gcccgcacgc cgaattgagg gccgtggccg cgtatgcctt
     6541 gggcgtcgtc ggcggcgagg aggcgacgaa ccggctgaag gcgatgctct atgacaccta
     6601 tcccaacgcc cgctacaacg ccgcgaccgg cctggcccgc gcgggggacg ccgagtgcga
     6661 gcgggtcttg aaggaaatgc tcgacccgga aaacgaggtg gctattcgcg acgagcgcta
     6721 taaggacgac caggcccgca agcggacgac cgtgctgctc aacggtgtca aaggcacgct
     6781 gatcctggcc gagcggaatc cccaggccga tctgtcgcgc ttgaaggaat cgctggtcag
     6841 gctctccaaa tcgccgctcg aaaacattca gatcgagcgc aacaaggtga agcgagccgc
     6901 gagcgaagcg ctggagcggc tggacccgag cgcgaagcgt tagcgagatg aaacgcatca
     6961 aaagcggcgg tggttggcgg gcgatcctct attcgctcca gaagtcgcgc caaatgggcg
     7021 gcctgtgggg cctctggaag gcgatgcgca gcaagaacgc ctgcaagacc tgtgcgctgg
     7081 gcatgggcgg ccagaagggc ggcatggtga atgaggccgg cagcttcccc gaggtctgca
     7141 aaaagtcgct gcaggcaatg gccgccgaca tgcagggcgc aatcaagccc gagttctgga
     7201 gcacgtactc ggtcccgcag ctgcaagcct tctcgccgcg cgaactggag tcgtgcggcc
     7261 gcctcgtgca gccggtgctg ctcgaaaacg ggtcccaata ctaccggccg atcgaatggg
     7321 atgacgcact gggccgcatt gtcgccaaac tgacggggac agcgcccgat gaaaccttct
     7381 ggtatttcag cggccgctcg agcaacgagg cgggcttcct gctgcagctc ttcgcccggc
     7441 tgtacgggac caacaacgtc aacaactgca gctacttctg ccatcaggcc agcggcgtcg
     7501 gcctttcgtc gtcgctgggc accgggacgg cgaccgtcac gctggaagac gtcgagcacg
     7561 cggacctggt cttcctgatc ggcggcaatc ccgccagcaa ccatccgcgg ctgatgacga
     7621 cgctgaagaa cgtccgccgc cggggcgagg tcatcgtcat caatcccgtg attgagacgg
     7681 gcctcgtcaa tttccgcgtg ccgtccgatc cctggagcct gctatttgga accaagatcg
     7741 ccacgctcta cgtgcagccg cacatcggtg gtgacttggc gctgatgacg ggcatcgcca
     7801 agcggatcga cgagatgggg gctcaggacg aggcgttttt gcaggagcat tgccggcacg
     7861 cggaacagtg gctggcgcac ctgcggcaga tcccgtgggc cgggattcac gccaaatcgg
     7921 gcgtcgagca ggccgaaatt gaccagatcg ccgagcgcta tgccgccgcg aaagacgtgg
     7981 tgtttagctg gacgatggga atcacgcacc acgcccatgg agtggccaac gtgcaggcga
     8041 tcgccaacct ggcgctgatg cgcggcatgg tcggccggcc gcgcgccggg ctgatgccga
     8101 ttcgcggcca cagcaacgtg caggggatcg gctcggtcgg tgtcacgccg cagctcaagc
     8161 aagcagtgtt cgacgctctg gagcgcgagt tggccgtcaa gctgccgacg actcccggcc
     8221 tcgacacgct cggctgcatc gaagaggcgc acgccggccg gctgaaggcg ggcttctgcc
     8281 tcgggggcaa cctgtatggc agcaatcccg acgcggcgtt cgccggggag gccctcggcc
     8341 ggctcgacca aatcgtctat ctcaacacga cgctcaacat cggtcacgcc catggcctgg
     8401 cgcgggagac gatcattttg cccgtgctgg cccgcgacga agagccgcag ccgacgacgc
     8461 aggaatcgat gttcaactac atccgcctct ccgacggcgg cccgcggcgg cacgccggcc
     8521 cgcggagtga ggtcgagatc atcgcggaaa tgggcgagcg ggtgctcgac agtagtccgg
     8581 ggtacccgcc tggcgggtcg gctccgcgag atgcggacct cacgcggagc gcgagggcta
     8641 ctatcgactg ggccagcatg cgcagcaccg gcaagatccg ccaggcaatc gccaaggtcg
     8701 tgcccggatt tgcgcagctc gcccagatcg accgcacgaa gcaagaattc cacattcccg
     8761 gccggacgtt ccatcagccg aaatttgcca cagccgacgg aaaggcgatc ctgcaccttc
     8821 acgagttgcc ggagttggcc ggcggcgaga acactctgcg gctgatgacg gtccgcagcg
     8881 agggtcaatt caatacggtc gtctacgagg acgaagatct gtatcgcggg caggagcggc
     8941 gcgacgtaat cctgctgcat ccggaagatc tgcggcggct gaatctcacc gagaaccagc
     9001 gggtcaccgt ttccagcgcc gcaggagagc tgcggggcat cctcgcccgc tcgttcccgg
     9061 aaatccgcgc cggcaacgcg ctgatgtact atcccgaggc caacgtactc gtgccccgcc
     9121 acgccgaccc gcattcgcgc acgccggcgt tcaagagcat cgtggtgagc gtcaccgcgg
     9181 aaattccggt gacggtctaa cgggaggccg aggctcccac gaagccgcgg cccaggcttt
     9241 cagcccgcca acgcagataa gattgtccgc cccaccaaat ccgttgccga gccagaccca
     9301 tcgggttatg ttgatgggac cacttggccg aagaacacgc tggcgatgcg ccacatacac
     9361 ttcctactcc tcgcagcaat catcaccgcc ggcggcactg ccgcgcgtgg ccagtcacca
     9421 gccgaatcgc cccgcttcag ccgccacgtc gtcgccgtct ttagccggtt gggttgcaac
     9481 agcggctcct gccacggcgc ggtgcagggc aaaaatggct ttcgcctgtc gctgttcggt
     9541 gccaggccgg aagacgacta cgaccagatt gtgcggcagg cgagcggccg gcgcgtgaac
     9601 ctgctcgatg tcgagcagag cctgctgctg ctcaaggcga ccggcaggct gccccacggt
     9661 ggcgggcggc ggatggaagc cggcagcctg gaataccggg tgctgcggaa ttggcttgcc
     9721 gccggcgccg tggccgacga tgtcggggcg tcgcagctgg tatcgctcga agtgacgcca
     9781 cgcgagcagg ctgcccgggc cgtcgaaaag tacgagctgc ggctcgcggc gaaattcgcc
     9841 gacggtagca cggaagacgt taccgcgctg gcctcgttcg cgtcactcga cgccggcgtc
     9901 gcaaccgtcg atgctgtggg ccgcgcgcaa gcgcagggcg tcggcgatac ggcgctcgtg
     9961 gcccgctttc gggccgatcc ggcggtgtcg ctcgtcgtcg tggcgcgcga gggcggcggg
    10021 cagcttgccc tgcccgatcc gccgagtgct atcgacggcc acatcctggc gaagctccgg
    10081 cgactgaact tgccgccggc ggaattggcc gacgacgcga cgttcctgcg gcgggcgcgg
    10141 ctcgatgtca ccgggcaact gccgacggcc gaagaggtcc gcgaattcct ggccgacacg
    10201 agtcctgata agcgcgagcg gaagatcgac cagctgctgg ccgagccggg ccatgccgcc
    10261 ctttggacgc tgaaattctg cgatctcctg aaggccaccg atttcggggt ctatgccgac
    10321 gggctcgacg agcagcacga agcgccgcgg tttcaagcct ggattcgggc ccgcctcgcc
    10381 gagaacacgc cctacgacga attcgcggcc cgcatcctga cggccaccag ccgcgagggg
    10441 cgtagtctgg aagagtggtc ggccgaagtc gttgcgctgg ccgagggcta tgcgacgccg
    10501 cgaaaggacc tggacctcta ttcccggcgg cagacgctcg acgcctattg gcagcggaag
    10561 gacgcggtcg gcgttccggg cacactgcag gttgcccacg cgtttctcgg gctgcggctg
    10621 gagtgcgccc agtgccaccg tcatccgcac gacgtctggc aacaggacga cctgctcagc
    10681 ttcgccaatt tcttcatggg cgtgcgggcc gtcggcttcc agggcgagaa cgacaagcgg
    10741 tatcccgacg agagccagct cgcaaaaggt tttgagcagg agggcaaaaa gctcgccgag
    10801 gaagtcaaac agctgaaaga aggccgcggc aaggagctcg cggaaaaggc caagcaggcc
    10861 cagcccgaga tcaaccgcct caagaacgaa atccagaagg gcggcgaggc ggcggcgctg
    10921 tccgaaatgc aacagcagct tgattctctg cagaccacta tcgccgagaa cgatcagctg
    10981 cagcgcgaga ttcgcgagaa agaccagcgg agcaagctgc tgaccgacgc gatccccaaa
    11041 cggatcgtcc acagccagat catgcatcag gccggtgatg agaagtcgcc tcgctttgcc
    11101 aaggtcacca gcccgctggg cacacagacc tcgcaaactt tccgtctgcc aggcgagcgc
    11161 gagcctgttg ccctgggcga gggcgaagat ccgcgggcca aggtcgtcga gtggctccgg
    11221 cggcccgaca atccgttctt cgccaaggcg atcgtcaacc gcgtttgggc gcactatttc
    11281 ggccgcggca tcgtcaaccc gcccgacgat ctctcgccac acaacccggc tacgcacccg
    11341 gcgctgctcg acgacctgtg gcgcgagttt gtcgccggcg gcttcgatct ccgccgtctg
    11401 caccgcgcga tcctctgcag ccgcacctat cagcagtgca gcttgccggc cgccgccagc
    11461 gccgccgacc gggcgaacta cgcctgtttc tatccccgcc ggttgcccgc ggaagtgctg
    11521 ctcgacgtcc tgaatcaggc gactgggacc cgggaaaacc tcgacatgcg ctacgaccat
    11581 tggccggacg gcatgtccac agtggagatt ccgtacaagc cgcggaacgg attcgtgaca
    11641 ttcatgcttg agcagttcgg gcggccgccg cgaaattcgg ccgtgcaatg cgattgcgcc
    11701 cggcagggcg acgcatcgct gctacaggtg ctgagcctgg ccaatcaccc gcgggtccgg
    11761 cagaaggtcg ccgatccgtc cggccgcgtg gcgtcgctga tccgggagac ggaagatccg
    11821 cagcagcgga tcgagcagct ctatttgagt gtgctctcgc ggctgcccga ccagctagag
    11881 tgctcggcct gttgcgagca cctcgcgacg actgcgacgc ccgaagaggg cttgcaggcg
    11941 gtattgtgga gcctgctcaa cacgcgtgag ttcatcctgc agcattaggc aaggaacgtg
    12001 acatgatcgg ctgccagacc ttccggcgac attcgcggcg cgacttcctg cggatcggcg
    12061 gggcatcgct gtgcggcgtg agcctgctgg atatgctgcg ggcgcagtcc caggcggccg
    12121 gcgcgccggc ggcgaaggcc aagagcatga tcgtctgctg gatggccggc gggccgccgc
    12181 acaccgacat gttcgacatg aagccggatt cgcccaccga ctatcgcggc gaattcaagc
    12241 cgatcaaatc gaagctgtcg gggctcgacg tgtgtgagtt gatgcccaag ctggccgagc
    12301 gagcggacaa attcacgatc atccgctcga tcaccaccat gaatcagccc ggcgaccaca
    12361 gccgggcgcc aatgtactgg ctgaccggaa atccgcggct gcccagcggc accgccgaat
    12421 atccgatgta cggcagcgcg gcggctaagt ttcggccggg gccggccgag ctgccgacat
    12481 ttgccgtgct gggcaagatc gatcatcaca tcggcaactc gatcgccggc agcttcctcg
    12541 gcccggcgtt caacccgttc atcttcgacc cgctggagaa gaaggacgac atcgccaaga
    12601 tgctcgcgcc gcagctggag ctgccggcct tcaccaagaa cgccgagctg ctcaaagcgc
    12661 tcgacaagcg gctgcgtcgg caagacggcg tcgatcccct gatcgccggc ctcgacgcct
    12721 accagcagac ggcgttcgac atgctccgct cgccgaagct gcgggcggcc ctcgacctgt
    12781 ccgaggagag cgacgaaacg gtggcccgct acaaggacca ccacctgagc aagtaccgct
    12841 atccctccgg cgatccgctg catttcctgc tggcgcggcg gctggttgag gcgggcgtgc
    12901 cgatcgtgca tttcagcctg ggctattggg actggcacgg cgagaatttc gtcgccgggc
    12961 ggcagcagat tccaatgttc gacgccgggc tctcgtcgct tttggacgat ctcgacgagc
    13021 ggggcctctt ggacacgacg atcgttctcg ccctgggcga gatgggccgc aagcccaagt
    13081 gcggcaccga gaagaacgcc ggacgcgacc actgggacta cgcccagttt gtgctggctg
    13141 cgggcggcgg cttcaagcgc ggctgcatcg tcggggccac cgacaagctc ggcgagcagg
    13201 tcaccgacaa cttctacaag atcgaaagct ttgggcgcac gctctatcac ttgctcggca
    13261 tcgatcctga cacgaccgtg tacacgccca gcaaccggcc gatcaggctg atcgcagagg
    13321 atgcgccgct ggttaaggag gccttgatct agcggcgatc gatcaggcta cagcgttttc
    13381 aaatactcga ggacggcccg cttctcgtcc tcggtcaggg cgtcggggaa atcgtggccg
    13441 gcggcgctct tgccaaagcg gtgcgtgtcg aagtgctgcc gtttcttcca gccggtgaag
    13501 accgcgtccg acggcagccg atcgaacgtt tcgatctgcc agccgacgcg cgtcgagtcg
    13561 aattcgtcgc cggtccgccg ccaaacggca ggccgctcct gcgggtgcag cacgtgccag
    13621 agcgtgggca cgctgccgtt gtggaagtaa gggctgctgg cccaaatccc gtccagcggc
    13681 ggggcaacgt agccgcccgg atcgctcttg ttcgccttct tgccgaactc cgcgaaccaa
    13741 ctctggccgt aggcgtcgcg gtgggccggc gtgagcgcgt caagccgcac gcggtcggtg
    13801 ccgacttcgg cgatcggcac gagcttctcg ggatagcctt cgccgtcgcc gtacgtgccg
    13861 tggcactcgg cgcaggtgcg gttgaagacg ccgcggccgc gctcggccag cggctgatcg
    13921 atccgatacg gatacctggg cggctgcaca gagctgaggt acgcgaagac gtcgcgaaac
    13981 tcctgctccc aggcgcggaa cttctcgggc ccattgctcc ggacgagcat gaactgcatc
    14041 agaccgcggt gccctttttc ggcgaagccg tcgatgtaga gcatcttctt ccgccggaag
    14101 tgccaccagg gaggcgcgtc catgtcgtgg ttggtgagtt tggcgaccgg ccggtcggcg
    14161 tggaacacga ggtccgcgtc gcggaagccc atcaggacga cgccgaacat cacggcattg
    14221 gtcgtgccgt cggtcgagcc gagcggtacg aacacgccgc cgacatccat ccgcgacagc
    14281 ggcttcgtta gctccgcttt caccaggcgc gtctcttcgg tcagcgtctg caaggcaaac
    14341 cgcgagttcg gcgctccggg ccagacctgc gtgccgcctt tgccgtcggg aatgagcccg
    14401 ccgtggcagg caaagcaatt catcgtccag ccttgccgat cgacgacgta ctgcagcggt
    14461 tgcgtgggat cgtccgggcg cggcgtcagg ccgtagcgct cgaaggccat ccgccgccgc
    14521 tcgtccgccg tcgccttctc ggcctccgac cgcagcggct cgggccactg ccgccagaca
    14581 ttgtcgaacg tctcctggtc aaaatccggc ggcagatagg ccttctgtgt gagcagctcg
    14641 tagccgcgct gggccgctgc ggagggctca gtcgcccgca gtggcgcggt tgacagcagc
    14701 acggcggttg ccaacagagt gaatcgcatg gtttgctcgc aaccgcaata tagccccgcg
    14761 ggcaacattt agccgccgca agtcagagct cgccaaagtt cgcggcccga atactcggcc
    14821 ccctggttgg gcaggggcgc gcgcggcttt tttcgcagtt ttcggtcgca actctcgaag
    14881 cgccagcgac ttgggtcacc agcacccaga ggccgttaag tactatttat gagtgttaga
    14941 gaactcgttc ggtcgccggc tgagctgctg cgacccaccc ggcctcgtcc gaatgtgaag
    15001 gcggtgagcg gatcggacct ccattggccc agggacgccc acgtgcttgc gacttgtctg
    15061 aatccgagct ctccgtggcg atctgacgca acctatccgg atggcttgtg tcgccgggcg
    15121 gttctcgcag cccgggcgaa ttgtaacaat ccgttcagta tctgtcaagc gcaacacaac
    15181 agattgtgtt tcgccgccgc ccaaccacaa ccggttgtgg tcgcgccttc taacgacccc
    15241 cgaaagaagg gcggcccaag tgccgccccc gatcccgcta atgctttctc ttcgggggca
    15301 aaaagccgat gcagaagcac acgaacatca gcccccagaa caggatgaac tccggccgcc
    15361 gccagctgtg gaaattgagc acgtgcggaa tgtgcaacag cttctgcagc tcgttttcct
    15421 ttgcgggcgg cggctgagcg gcgggcactc ctggcggggg aggcggcgct ggctgcacgg
    15481 gctgctccgg ggcggccacg tgctgcgctt cgagtcccag cggccacttc gttcgtagcc
    15541 agtgttcgta gctgtgcgcc cagccctgcc ggcgcttctc gaagttctca aagctctttt
    15601 tccagcccca tttttcgagc tgctgggcaa ccacctccgg ccgatcgaag ctccaatcgg
    15661 aattgacgcc ccaatcggcg agcagcttca ggccgatcac gatcacgagc aggtaggccg
    15721 acagctcgaa gcgcgggaac ttttccagca gccagatgaa ccacttggcg gcgactcgca
    15781 tcagcatcag gccgatgatg ccgccaaaaa tgacgaccca cagcttgggg tgcagcgcgt
    15841 tgggaggatg gccgcggggc ggtgcgccga ccatggcaat ggcggccagg atcgaatcga
    15901 ccgcgaaggc aatgtcggtt agctcaatga ccagcaccgt gggccagaag ctcgccaggc
    15961 cgaattgctt gcgcctctcc gccgacatat agaccggcat gcgccctcgg atttcgactt
    16021 cttcctgttc gtcggtcagc ggcccgccgg ttctttcctc gaccaggacc ggatggccgt
    16081 gctcgtcgag ttggatcttc tcatcggcct tttccttgga ctcgaagaac aagtggcgga
    16141 ccgcgatata gacgagatag gccccgccca gaaacttggc aaaggtccac tgcagcagca
    16201 ggctggccag cagcaccgcc aggaaccgga agacaaaagc gccggccagg ccataccaca
    16261 gggccttgcc ctgctcgctc ttgggcagcc gcttggccaa gaggcccagc accagggcgt
    16321 tatcgatcga gagcagtccc tcgagcagca ccaacagggc cacgaccgcg agatccggcc
    16381 agcccacctg ctggccgaat atgttgataa atcccaggag tagcatcaga ttggtgcggc
    16441 ggcggacaaa caaaaatggg tggcggttgc cacccaggac gcattcatct taaccgttgc
    16501 gactacggct catagcccag gttcggggcc agccagcgct cggcttccgc gagcgacatt
    16561 cctttgcggt gggcgtaatc ttcgacctgc tcgcgggtga tgcggtcgac ggcgaaatag
    16621 cgggcctgcg gatgcgcgaa atagagtccg cagacgctgg cggccggcca catggcgtag
    16681 ctttcggtca atttgatgcc ggtgtgctct tccgcgccga gcaagtcgaa cagcgttttc
    16741 ttctccgtat ggtccggttg cgagggatag cctggcgcgg ggcgaatgcc gcggtactgt
    16801 tcgtcgatca gctcatcgtt cgaaaagcgc tcggccaggc cgtaacccca gtcgcggcga
    16861 gctcgctgat ggagcaattc ggcaaatgcc tccgcgagcc gatccgccaa cgccttgacg
    16921 aggatcgaat tgtcgatctc gtgccgcgcg tcgaaccggg cgcacagctc gtcacacccg
    16981 acgcccgttg tgaccgcgaa cgccccgacg tagtcctggc gaccgctgcc gagcggcgca
    17041 acgtaatcgg ccagcgcgcg aaaatccttc tggccttgac gctcccattg ctgccgcagg
    17101 gtgtgcaatt tcagatttgg gattggggat ttgggattta ctggctcctg cacaacgatg
    17161 tcgtctccgt cactgttcgc cggccagaag ccgtagacgc cgtgggcggt cagcagccgt
    17221 tcgtcgatga ttcggcggag catctgctgg gcttcctcga acagggcgcg ggcgtgcggt
    17281 ccggcggcgg ggtcgtcgaa aatccgcggg tacttgccct tcatctcgta ggtcaggaag
    17341 aacggcgacc agtcgatgca tttggcgatc tcggccagcg ggaaatcgcg gagcgtgcgg
    17401 atgcctgtga aggcgggcgt ggggatatcg acctcttgcc aatcggtctg aaaacgccgc
    17461 gcgcgggcct gagccagcgg gacgagcttt acgtcgcgtt ggcggtacga ctcggccagc
    17521 tgctgctgca gactgcggtt ctcgcggtcg agctgcggct tgtgctgcgg gctgagcagc
    17581 ttatcgacca cgcccacgca ccgcgaggcg tccagcacgt ggaccgtcgg ctgctcgtac
    17641 tgcggggcga tcttcacggc cgtgtgcttg gcgctggtcg tcgccccgcc gatcaagagc
    17701 ggcaggcaca ttcgccgccg ctgcatttcc ttggccacgt aaaccatctc atcgaggctg
    17761 ggcgtaatca ggcccgacaa gccgacgatg tccacgccct gctgcgtcgc ggtgtcgaga
    17821 atcttctcgg tcggcaccat cacgcccagg tcgatcactt cgtaattgtt gcagccgagc
    17881 accacgccga cgatattttt gccgatgtcg tggacatcgc ctttgaccgt cgccagcagc
    17941 accttgccgc gggccttctg ggcagcgagt ccggccgagc gtttttcctc ttccatgaag
    18001 ggcagcaaat agccgaccgc cttcttcatc acgcgggcgc tcttgacgac ctgcggcagg
    18061 aacatcttgc cggccccgaa caggtcgccg accacttgca tgccggccat cagcggccct
    18121 tcgatgatcg acaggcagcg gggatattgc tggcgggctt cctccgtgtc ttgctcgatg
    18181 aacttgtcga ttcctttgat tagtgcgtgc ttcagccgct cctcaaccgg cgcgtcgcgc
    18241 caggcgaggt cctcggtcgc ggcggccttg cccttgcctt tgacccgctc ggcgagctgc
    18301 aacattcgat ccgtcgcgtc ggggcggcgg ttgagcagca cgtcctcgca gtggttcagc
    18361 agctctttcg gaatctcctc gtacagggcg agctggccgg cgttgacgat acccatgtcc
    18421 atgccggcgc ggatagcgtg atacaggaat gccgaatgca tcgcctcgcg gaccacatcg
    18481 ttgccgcgga aggaaaagct gatgttgcta acgccgccgc tgatcttgca gcccgggcag
    18541 cgctccttga tcagccgcgt ggcctcgatg aagttgaccg cgtagttgtt gtgctcctcg
    18601 atgccggtcg cgaccgtgag gatattcggg tcgaagatga tgtcttcggc cgggaagccg
    18661 accttgatgg ttaggacccg gaaggctcgc tcgcaaatcc gcaccttgtc ggcaacctca
    18721 acggcctggc cgttctcgtc gaacgccatt acgaccaccg ccgcaccgta gcggcggacc
    18781 agtgccgccc ggcgaaggaa ctcctcctcg ccgtccttga ggctgatcga gttgacgatg
    18841 cctttgcctt gcaggcattt cagcccggcc tcgatgacgc tccacttcga gctgtcgatc
    18901 atcaccggga ccttttgcac ttcgggatcg cccgcgatca gccgcaagaa gcgggtcatc
    18961 gccgcctcgc cgtcgagcag ggcgtcgtcc atgttgacgt cgatgatcgt ggccccgccc
    19021 gcgacctgtt gccgggcgac ctcgacggct tcctcgtatt tgccgtcgcg gatcagccgg
    19081 gcgaacgcct tgctgccggt gacgttggtc cgctccccca ccatgatgaa gttgctgtct
    19141 ggccgctgcg tgaaggggag cgtgcccgaa agccgcagcc actgcggctg ctcggcgggc
    19201 tcgtgggggc gcacgcggcc ggcgacttcc gccagggccg cgatgtgctc gggcgtggtc
    19261 ccgcagcagc cgccgacgat attcagccag cgattttcga tgaactcgcc gacgaggcgg
    19321 gccatcgcgg cggggcccag gttgtattgc ccggtgactt tgtcgggcag gcccgcgttg
    19381 ggatagcagt ggacgcgggc cgtcgcgacg tgcgacagct cctcgatgtg cggccgcatc
    19441 gtctccgggc cgagggcgca gttcatgccg acggtcaata gcggcacgtg attcacggcg
    19501 ttccaaaagg cctcgaccac ctggccggag acaaacgtcc cgccgccgtc gccgaacgtg
    19561 cccgacgcca tcaccggcag ccgcacgccc cgctcctgaa agcagcgctc aatggcgaac
    19621 aggcaggcct tgaggttgag cgtgtcgatg aacgtctcgg gcagcaggat atcgacgccg
    19681 gcctcaacga gcgccgcgac ttggtcgtaa tacgaatcga ccatctggtc gaaggtgatc
    19741 gcccgccacg ccggatcatc gacgcgtgtc gagatggcca tttgcttggt cgtcgggccg
    19801 atcgagccgg cgacaaaccg cggccgctcg ggcgtccgct cattgaattc gtccaccgcc
    19861 tggcgggcgc aggccacggc agcaaagttc agctcgcgga ccatctcgcg cggaaagcgg
    19921 aactcctgga tgccgaccag gctggccccg aaggtgttgg tctcgacgat gtccgccccg
    19981 gccgccaggt attcgcggtg aatctgcgtg atgtcgtccg gccgagtcag gcagagcaga
    20041 tccgagaaat tgacgagctg gatgtcggga tcgccctcgc agtggtcgcg aaatcgctcg
    20101 ccgcgcttgg cggcctcgtc cagccccagc cgcatgatca tcgtccccat cgcaccgtct
    20161 aggacgagga ttcgctcggc aaggagcgcc tcgagctgtt cataggcttt ggtgcggacg
    20221 ggtgctggca tgcgaggaaa cgggtatttc aaggggaacc tcacatcatc ttacgcacgg
    20281 cacgaggcaa caaggagcga taactctccc gaagagcgcc aatccgcgat tgcagctagg
    20341 cagccaagca ttttccatgc gcttcccggc ggccaaagcg gcaaagtttg cccagttctc
    20401 aagatcaata caacccctac cgataaagga tttgcggctt ggaccgcgca ggccaattgc
    20461 gcgctgccgc cgcagggaaa ggacgcggga catgaatctg ctcaatgaac cctccaccgg
    20521 tgaagcgcgc cctaggctgc cggtgctgtt tcacctgctc gacgtcagcc ggcagcgccc
    20581 ttcgcgtccg ccagcggagc cttcgctagc gtccctgtcc gaaatggcgg agcccgctgc
    20641 ggcaacggcg gtcgcaccgc cggcagctga cctgtcgctc gcgaccccag cggtgcccat
    20701 cgagaccccg actatttcca ttccacctgc cgccccggag gcggccgcct ccaaatcgag
    20761 cgagccggcc aagtcctcgc cgccaacgcc gcgctccgaa gccgagcggc ggcgcaaggc
    20821 caagaggccc accaccgaag aatggttcgc ggcccacggc aagtacatcg cgctagtctt
    20881 cgtgctggca ctcggtgcca cgatctattt cgcccgggtc aaccgccggc cgccggccgt
    20941 ggaggagccg cccgacatgg cactgaatat cgagcccggc gacagcgccc gctcgaccgc
    21001 aaacagcaag ccctcgacca cgctcgtcgg cgactcggtg ccgaatccgg ccacttccac
    21061 tggcagcccc gcaaaactga tcgagaccgc ctcggccagc gccccacaag ccgaactctt
    21121 tccacccaca gaaacgcagg tcgccgcaag gaatccgtcc gctgccgccc ggccggccgc
    21181 gagcgacctc tttccggata agaaatccga ctcgagagtt gccgtgcaag atcgcggcca
    21241 agacgccctc gccaacagct cgccgacaaa ttccgccagt ccgctggcgt cagccgcacc
    21301 agccgctcct gcgacggcat ctgcaccagg tgccgcaccg gcggctgcca cagcgtcggc
    21361 gatggaaccc tcctacccgc acacgggctt tccgccgacc gggttgcccg cggccagcta
    21421 ccccaccacc catccacccg taacgaacta cccgcccacg aacattccgg gccacgctgt
    21481 gccggccccg cccgcgatgc ccgcgcagcc gccagcgtgg tcgcactccc cgtatcaacc
    21541 gtcgccatac tcgcctaccc agttccagcc accgcaatca ccgacgcaat tccagccgcc
    21601 gcaatcgccg acgcaattcc agccggctca gcgtccgccc ttaggctacc aaccggtttc
    21661 gccggccccc ggcggcagct tcgccccgcc cggtggcgcg atcccgccgc agtaccagcc
    21721 tccattcaac tccgtccccg gaactcgcta tgagcgctct ggaccaggcc tttattaagg
    21781 cttatgccaa agatcagccg gccgatcggg cggcagccgt agcgagccga ggtgcgccgc
    21841 cgcgggcggt cgccggcgcg cgggttgcgg caggcgcgat cgaacaggtc taccgcgacg
    21901 gctcgctcta ccgcgtggac ttgcccgcgc ggatctcgcc gtccgctgcg gcgattcctc
    21961 agccgcacgt caaactgttg ccgccgacct cgccgcgacg cagcgtccag cggtcgctgc
    22021 tcaggctgct ggccaatcaa cccgtgccgc cgccggcaga tgccgagctt ccgccgaagg
    22081 ttgcgcggaa ggtcatcatc cggcacattg cccattcgat tgcaccggcg ccgctgggcc
    22141 tgctgcgccc cgaggtcggt gagccgctta cgctggcaga agtcgatgaa ttgccgccgg
    22201 agacgatcat cgagtggccg cccgcgccgg ctgttgcgcc gccacccgcg ccaattgccg
    22261 ccccccagac ggcgccgctc aacgaggcgc acgcgattcc ccaacttgtt gaggaacggc
    22321 cgatcgccgt tcacggtcag tgggcgttgg ctgaatcggc ggcggggctg gtcgtagtgg
    22381 ccgagccggc ggaattcgac gaggccgggt cggacggcct ggtagccgtg cgactcgaat
    22441 cgctcacgct ccccgagccg ccggctgctg aaccgtttgc agaggaatcg ttggtcgagg
    22501 cagcggattt cgaacttcac gaggcggagc gtacgcggtt tgcgggcaag tcgctggacg
    22561 atgccgataa cggcgagccg gccttccggg tcgatccgcc gcatgcggca tccattcatc
    22621 ggccgcacgc tcgtttcacg gccgagccgc agccagtgga cgagttgacg ctggccgaaa
    22681 cgccgccgga gccgcagacg caagctgagg cagctgcgcc cgtagagtcg ccagcggccg
    22741 agcccgccag cgctacgtcg gtcgcgccgc tgtgggaagt cgatcggttt cactggccga
    22801 agacctgcga gaaactgctg tcggccgagg gaagctattt cgtccacgcc ggcgcaaagc
    22861 tcgtggccgc agtcaaggac ggcctgcgcg tgctggcgat caccggcacg cggcggggcg
    22921 aaggccgaac gacgctggcg ctctgcctgg cccgcgtcgc cgcccaggca ggcgtgcagg
    22981 cggcgatcat ggacgcggac tttgcccggc cgcaattggc cagcaagatc ggcctcgcca
    23041 tcgctcacgg ctggcaggac gcggccctag ggaagattcc gctcagtgaa gccgccgtga
    23101 agtcgctggc cgatcaggtc accgcgctgc cgctggaggc ggccggcgcg gcttcgcgat
    23161 tgtcgctggc cgatccgcgg gtgaccgcga cgatccgggc cgccgccgcg acgttcgagc
    23221 tgctgattct cgatttgggt cccgtcggtc cgggtgagga agttgctttt ccgcctggcg
    23281 agaagtgccc gctggatgcg gcgatcgtcg tccgcgacat gcggtttgcc acgctcgcgg
    23341 agagcgaggc catcggtcac ggccttagcg acgccggcgt cgaggcggtc ggcatcgcgg
    23401 agaatttcgt ggtggaagag gaaccgcaat actcatcgtg attgctcgcg atgcagtccg
    23461 ccccgagcta tgtacgaagc ctactggcaa ctcgacgcgc ggccattcga gcatacgctc
    23521 gacgagcgtt tccattttgc cgccgagtcg cagcagggag cggtcctcaa gctgcggtac
    23581 gttctggaga accgccgcgg ggcggcgctc ttagccggcg aatcgggcct gggcaagacg
    23641 ctggtggccc agtcgctcct ggcgcagctc agccccgctc accagccgcg attgcatatc
    23701 gtttatccgc aaatgccggc cgagcaattg ctcgcctatc tggccgatca actgacgggg
    23761 caaaccgcgc ctctggcggc tggtgttgac cagtgcgtgc tgcgaatcga gcaagccctt
    23821 cgcggcggcg ctacggaggg ccggcacgcc gtcatcgtca tcgacgaggc tcacctgctc
    23881 cgccagacgc aagccctcga gtcgatccgc ctgctcacga acttccagca cgaggggcgg
    23941 ccgctggcga cgtttctgct ggtcggccag acgggcctgg ccgtcgccac ggagcgctgg
    24001 ccgcagctcg acgagcggct ggccgtcaag tgcattctca cacggctttc ggcggccgag
    24061 acgcggggct acatcgagca ccgcctgtcg gccgccggcg cgaagcgacc gatctttagc
    24121 gaaaccgcct tcgccacgat tcacgaactg acgcagggca ttccgcggcg cattaaccgg
    24181 ctctgcgacc tggcgctgct cgtggggtac ggcgaggagc tgtcgatcct ccagccggag
    24241 cacctggagt cgatccacga agagctgatc ggccgggcgg cgacagcggc gtagggcagc
    24301 cccgttgccc atggatgctc acgtccccgc gacgtaacgc ggatcgatct tctcgatctc
    24361 ctccgccacg gcggccaccg tgctgcgaat ctgctgctcc gtgtgcgcgg cagtgatgaa
    24421 gaaccgcagg cgggccttct cttcttcgac ggccggatac aggatcggct gcacgctgac
    24481 gccgcggtcg aacagggcgt tcgacaaaag cagcgcgtgg tacgaattgc cggtgatgat
    24541 cggcaccacg gccgtgttat tgctcattcc ggtattcagc ccgcgctgcc tggccagctc
    24601 cagaaacaac cgcgaattgg cctgtagccg ctcgatccgc gaggggtcgc gctgcagcag
    24661 gtgtagtgaa gccagcgccg ccgcagcgcc gggcggcgaa aggccgcagc tgaagacaaa
    24721 gccgggcgag gtgtatttga gatactcgat cagctccttg gtgccggcga tgtagccgcc
    24781 gcagctgccc atggccttgc tgagcgtgcc catcaggaga tcaacgtcgc gcggatcgac
    24841 gccgtaatgc tcggtcaggc cgcgacccgt ctttccaagc acgcccagcg agtgggcttc
    24901 gtcgatgaag agcaaggcct tgtgccgctt cttaatttcg acgaatggcg gcagttcggg
    24961 gaagtcgccg tccatgctgt aaacgccttc gatggcgatg accacgcggt ggtagtcgcg
    25021 gcggacctcg gccagcaatg cgtccaccgc ccgccagtcg ttgtgcggaa acggccgccg
    25081 gcgagccccc gagagaaccg aaccttgaat gatgctgttg tgggccagcg aatcgtgcac
    25141 gatcaggtcg ccggggccga acaggtgccc gagcgtcgat tcgttcgtgc agtggccgcc
    25201 gatatagcag atcgaatctt ccaggccgtg gaaccgcgcg atctcgcgct ccagctcgac
    25261 gtggaccgtc ttctcgcccg aaaccagccg gctggccgac gtgctggtcc cgaactggtc
    25321 gatcgccacc ttcgcggcct gggcgatctc cggatcgccg gacatgccca ggtagttgta
    25381 cgtcgcaaag ctgatcagct ctcggccgtc gatccgggcg atgtcgcggg tcgccccttc
    25441 gtgcgcccgg aaatacgggt tgggcacgcc ggtcgagccg agcatcgcca tcgtctgctt
    25501 cagccgcttg tattccggca tctggctgaa gtcgtaacat tccggcggaa tctcgcctgc
    25561 tgaggcccaa ttggcagtag gcccggccgc cgcgtgccgc tgcacgcgcg gctcggtgcc
    25621 gatataggtc gcgatcgccg cggcgacctc ccggacggtc tcgatctcgg ccagcacgtc
    25681 ctcaggaaac cgcccgccaa acgtctcttc cagcgtgtta gcgatctgca gccgctcgag
    25741 cgaatcgagc cccaggtcgg tgacgatatt tgaatccagc gcgagcacct tggcccgctc
    25801 cttggcgatc gccctgacgt ggtccatcac aatctgggcg atggcgggat cgacgtcggc
    25861 gagcgacttg ccttcggcgg attctgccgg agcggaggcc gcgggctgct cctccgcgcc
    25921 ggcttcccag gccttccact gctgaatgat ttgcagcgag ccgcccaaaa actcttcgcg
    25981 gcaggcgtgc cgctggatct tgccgctgga ggtcttgggc accgatccga accgtacgag
    26041 gatcaccgcg tccggcggca gctcgtgctc ggcggttacg gccttgcgaa cgtcggcgat
    26101 cacgtcgctc cagtcgtcgc ggcgggtccg ctccacctcg gccacgatga tcaatcgctc
    26161 ccggccttcg agatcgaccg cgaacgcccc caccgcttgc ggctgaatcc gcgggttggc
    26221 ccgctccacg gtcatttcga tgtcctgcgg atagcggttc acgccgcgga cgatgatcag
    26281 gtccttgatc cggccggtga cgaacaattc cccctggtcg aagaagccca gatcgcccgt
    26341 ccgcaaatac gcccggccgc cgtcgccggc cagatgcgcc cggaaggtct gctcggtgtc
    26401 ggccgggttg ttccagtagc cctgggcgac gctagggctg ttcacccaga tttcgccgat
    26461 cttgtccggc cgcaattcgc gccgcgacaa gggattgacg attttcacgt gctcgtcggg
    26521 caggacgtga ccgcagccga cgagggcccg cccgccggcc gcgctggccg ccacgggcac
    26581 gacgaccttg gcgtcgagct gccgggcgtc gaacgagcgg acgattttcg gccggttcgc
    26641 atagctgccg gtgacgatca gcgtcgtctc ggccatcccg tagcagggat agtgcgactc
    26701 ggcccggaag ccgtaggggc caaaccgctc ggtgaaccgc cgcagcgttt cggcccgcac
    26761 cggctcggcc ccgttgaagg ccacctccca cgcgctgagg tccaggccgg cgagctgatc
    26821 gtcggtgatc ttgtcggtgc acaggtcgta ggcaaaattc ggcccgccgc tgaccgtcgt
    26881 gccgtatttc gtgatcccgc ggagccagcg gatcggcttc tgcaaaaagg ccatcgggct
    26941 catcagcacc gcgggccggc cgaagaacat ggcaaacagc gtcccgccga ccaggcccat
    27001 gtcgtgatac gtcggcagcc acgacaggcc gctgcccttg cgatgcacct cgaacgagtg
    27061 gcagatcagc tgcacgttgt gcatcaggtt ggcgtgcgag agcatcacgc ccttgggtgt
    27121 gcccgtcgag ccgctggtgt attgcagcat ggccagcgag gccgggtcga tccgcggcgg
    27181 attccagccg gccccttcgg tgtcgctcac gcggtcggtc gccagccagt gcaactgttt
    27241 caggctcggg gcgtcttcca gcaggtcctc gatccggccc agcacgtcct gctcggtgag
    27301 ggcggcctgg gcctggcaat cgtcggcgat cgccttgatc cgctccatat tgcggttccg
    27361 ccgcggcgga aacgccggca ccgcaatcac gccggcatac aggcagccga accaggcgac
    27421 cacgaactcc agcccctgcg gatagagcag caaggcccgc tggcccgtca atcccaggtc
    27481 cgtgagccgg gccgcgatgg cccgcgcgtg gcggtcgaac tgctcgtagg tcagcgcctg
    27541 ctcctggtcc tcgccgtcgg tgtagtaata ggccgtttcc tgcggctgct gctcggtcca
    27601 gtagcgcagc gaatcgatga gggtcgtagt cggcggcgaa aacggcgcga cgaagcgttt
    27661 caggtccacg gaaccgcaat ctccagcggg gaacggggaa actgcgattc taacggggtc
    27721 agttgtcagt tgtcagtagt cagttggagc agagccaccc caactgacta ctgacaactg
    27781 acaactgacc actgactacc ccggcaccgt cgcgccgacg gccgcgctcc acggcccggt
    27841 ctcgccgcgg gtgctgaccc agcggagcca gtaggtgaca agcttgccgc cgtcggcccc
    27901 ctcgaagggg atcgtgtggg gcgtccgcgt gtcgagcgtg acgaagacca ggtcgccggc
    27961 cgcggccggc gggggcgtgc cgatcttcat ccagatctcg cagccccgca cgcccgcggg
    28021 tttggccttg ctggtgggcg tggcgctatc gacgaagctg accgtatgct gcaagcgctg
    28081 cgacgtatcg acctgcccga ccggcgcggt ggtcggcgga ccgctcggcg tcggcgtcgt
    28141 atcgggcacc gtaatccccg ccgccgcctt ggctgagccc gtgaccgccg gcgtggcctg
    28201 aattttcctc acgacggtcc gcacggccgc caccagcgac tcgcgctggg cgtccttggt
    28261 ctgcgtcgcc gcctcggccg cggcctgggc cgtctgctgg gccgcgaagc ccgtgttcca
    28321 ggccgtctga gccgtcgtca ccggcgtcag gtccgcggcc accagcccat atccgg
//
LOCUS       DASRJB010000027         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3393857, whole genome shotgun sequence.
ACCESSION   DASRJB010000027 DASRJB010000000
VERSION     DASRJB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3393857"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFB80_00720"
     CDS             complement(<1..911)
                     /locus_tag="VFB80_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZN32299.1"
                     /translation="MTTQIPSQLGYRWPAEWEPQASVWLSWPRNRNSWPNHFEPVPGE
                     FAQFVRLLADYEPVNILAGGREVMEQARSLVGDLKNVTLHDIPTNDAWCRDHGPTFLS
                     SPHAPREGDISRGEMATMPALVDWEYNAWGGKYPPFDLDNQVPAKIAALQGRRRFSPG
                     IILEGGAIDGNGAGTILTTESCLLNPNRNPQLGRADVESLLRDYLAAQKILWLTGGEI
                     AGDDTDGHIDQIARFVGPRTVVVSICDDEGDENFEPSQQNWRELQSLTDQEGRPLELV
                     PLRLPRALFCDGQRLPAGYCNFLIANGV"
     gene            1083..1487
                     /locus_tag="VFB80_00725"
     CDS             1083..1487
                     /locus_tag="VFB80_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN32300.1"
                     /translation="MAHFDRVIPVLTYQDIPAAHDFLVRAFGFAAGGVHRTPAGQPIH
                     GEVRAGGAAIWLHRVTAEHHLDSPLAADVANTGLVVHVDDVDAHYQQARAAGARIDSE
                     PVDQPYGQREYGARDLEGHRWWFATPVSKALV"
     gene            1579..2244
                     /locus_tag="VFB80_00730"
     CDS             1579..2244
                     /locus_tag="VFB80_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN32301.1"
                     /translation="MYELDHVFVWVDAGGAVADRLVSFGLKEGEPNVHPGQGTANRRF
                     FFRHAFFELLWVADAAEAQSQLVRPTQLYERWSLRNSGVSPFGVALRPTDRGPAEPPF
                     PAWQYRPPYLPSPLAIHVGQNVPLMEPWWFFVGFGRRPDDPRWPKPQPLAHAAGFREI
                     TQVRFTVPDLASPSLSAAAAVRTGAVALASGPEHLMEMTFDLGQQGQAKDFRPALPLL
                     FLW"
     gene            complement(2283..3173)
                     /locus_tag="VFB80_00735"
     CDS             complement(2283..3173)
                     /locus_tag="VFB80_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HZN32302.1"
                     /translation="MPYDHVRNRPVTVALVQMSCTEAKEPNVEKALAKIAEAAGKGAN
                     IVCLQELFTGPYFCQSEDHRWFAEAEPIPGPTSERLAAAAKQHGVVVVGSLFERRAPG
                     LYHNTAVLLDADGSQAGVYRKMHIPDDPLYYEKFYFTPGDLGFQSFDTKFGRVGVCVC
                     WDQWYPEAARLTALSGAEIIFYPTAIGWLPAEKEEFGASQHNAWETMMRSHAIANGVF
                     IAAPNRVGREGQLQFWGGSFVSDPNGNLLAKAGHEREEIVLVKCDLGLIDVVRTHWPF
                     FRDRRIDAYGELTKRYRDTP"
BASE COUNT          585 a         1140 c         1087 g          615 t
ORIGIN      
        1 acgccattgg caatcaggaa attgcagtag ccggctggca atcgctgtcc atcgcaaaag
       61 agcgcccgcg gcagccgcag cggcacgagc tcgagcggtc ggccctcctg gtcggtcagg
      121 gactgcagct cgcgccagtt ctgctgtgac ggctcaaagt tctcgtcgcc ctcgtcgtcg
      181 cagatggaga cgaccacggt ccttggcccg acgaaccgcg cgatctggtc gatgtgcccg
      241 tcggtatcgt cgccggcgat ttcgccgccg gtaagccaaa ggattttctg ggcggccagg
      301 tagtcgcgca agaggctctc gacgtcagcc cgcccgagct gcgggttgcg gttgggattc
      361 agcaggcacg actccgtcgt caggatggtg cccgcgccgt tgccgtcgat cgcgccccct
      421 tccaggatga tccccggcga aaaccgccgc cggccttgca gcgcggcgat ctttgccggc
      481 acctggttat cgaggtcgaa tggcggatac ttgccgcccc aagcgttgta ttcccaatcg
      541 accagtgcgg gcatagtcgc catctcgccc cgcgagatgt ccccctcacg cggagcgtga
      601 ggactactaa gaaacgtcgg cccgtgatcg cggcaccagg cgtcattcgt cggaatgtcg
      661 tgcagcgtga cgttcttcaa atcgccgacc agcgagcggg cctgctccat cacctcgcga
      721 ccccccgcca ggatatttac cggctcataa tcggccagca gccgcacgaa ctgggcgaac
      781 tccccgggta ccggctcaaa gtgattcggc cagctattgc gatttcgcgg ccacgagagc
      841 cagacgctgg cctgcggctc ccactccgcg ggccagcgat aaccgagttg gctgggtatt
      901 tgggtagtca tcgtcggatt gccaacgcac ctcactggct acaacttagc gagcccttgc
      961 ccaatctggc agcccgccaa aatggcgcga cgcgccgcga gattgccggc gtggtaaact
     1021 cggggcacgg cttccactgc caatctccat cgcggcgaat catactgccc tgaggtgcaa
     1081 ttatggccca cttcgatcgc gtcatcccgg tgctcacgta ccaggacatt ccggctgcgc
     1141 acgactttct ggtgcgcgcg ttcgggtttg ccgcgggagg tgttcaccga acgcccgctg
     1201 ggcagccgat ccatggcgaa gtccgcgccg gcggcgcggc gatttggctg caccgcgtca
     1261 ccgccgagca ccacctcgat tcacccctgg ccgccgacgt agcgaacacc gggctcgtcg
     1321 tccacgtcga tgacgtcgat gcccactacc agcaggcccg tgcggccggc gcccggatag
     1381 acagcgaacc ggtcgatcag ccctacggcc agcgcgaata cggcgcccga gacctggagg
     1441 gtcaccgctg gtggtttgcg actcccgtga gcaaagcact cgtttaacac ggagccggtg
     1501 gcgacggcgg ctgagggaca aggcgcgacg cggatggacg tcgatacctg aagtgcaagt
     1561 cccagcaagc gcagcgccat gtatgaactc gatcatgttt tcgtctgggt ggacgcgggt
     1621 ggcgcagtcg cggaccggct cgtttcgttt ggcctgaagg agggcgaacc gaacgtccac
     1681 cctggtcaag gcacggcgaa ccggcggttc ttctttcgcc acgcgttttt cgagctcttg
     1741 tgggtggcgg acgcagcgga agcccaaagc cagctcgtcc ggccgacaca gctttacgag
     1801 cgctggtctc ttcgaaattc gggagtgtct ccctttggcg tcgcgctgcg gccgacggac
     1861 cgcggcccgg cagaaccccc gttcccggcc tggcagtatc gcccgccata cctccccagc
     1921 ccgctcgcca ttcacgtcgg ccagaacgtc ccgctgatgg aaccctggtg gttcttcgtt
     1981 gggttcggcc gccggcccga cgacccgcgc tggcccaagc ctcagccact cgctcatgcc
     2041 gccgggtttc gtgagatcac gcaggtccgc ttcactgttc ccgatctggc cagcccttcg
     2101 ctgtccgcgg cggcagcagt ccgaacggga gccgtcgcgc tcgcctctgg gccggagcac
     2161 ctgatggaga tgaccttcga cctgggccag caagggcaag cgaaggattt caggccggcg
     2221 ctgccgctcc tattcctctg gtgatgagtg ctctcgtggc aggagccgga gcggtagcag
     2281 ggctacggcg tgtcgcgata tcgcttcgtc aattcgccgt aggcgtcgat acgccggtcg
     2341 cggaagaaag gccagtgggt gcggacgacg tcgatcaggc ccaggtcgca cttaactagg
     2401 acgatttcct ctcgctcgtg gccggccttg gccagcagat tgccgttggg gtcgctgacg
     2461 aacgagccgc cccagaattg cagctgccct tcgcggccga cccggttggg ggcggcgatg
     2521 aacacgccgt tggcgatcgc gtgcgaccgc atcatcgtct cccaggcatt gtgctggctc
     2581 gcgccgaact cttccttctc ggcaggcagc cagccaatcg ccgtgggata aaagatgatc
     2641 tcggccccgg agagggccgt cagccgggcg gcctcgggat accactggtc ccagcagacg
     2701 cagacgccga cgcgcccgaa cttcgtgtca aagctctgaa agccgagatc gccgggcgtg
     2761 aaatagaact tttcgtagta cagcggatcg tcggggatgt gcatcttgcg atagacgccg
     2821 gcctgcgagc cgtcggcgtc gagcagcacg gcggtattgt ggtacagccc cggtgcacgc
     2881 cgctcgaaga gcgagccgac gaccaccacg ccgtgctgtt tcgcggccgc cgccagccgc
     2941 tcgctggtcg gccccggaat cggctcggcc tcggcaaacc agcggtggtc ttcgctctgg
     3001 cagaaatagg gccccgtgaa caactcctgc aagcagacga tgttcgcccc cttgccggcc
     3061 gcctcggcga tcttggccag cgccttctcg acgttaggct ccttcgcctc ggtgcacgac
     3121 atctgcacca gggcgacggt taccggccgg ttgcgtacgt ggtcgtaggg catcggttca
     3181 ggagtcaggc gcaaagctca gcgaagtgtt tgatctcgcg gcgtagcatg caattattgc
     3241 ggaatagtgc tgggcgtaca tgggtgcgtc ctaaaccttg aactttgaac cttgaacccg
     3301 gaactcagaa ccccatgcct ctgacgccgg ccatccagac cgacgaactg cgccgccggg
     3361 cgcagcagct cggcttcggc aaaccagcgg tggtcttcgc tctggcagaa atagggcccc
     3421 gtgaaca
//
LOCUS       DASRJB010000028         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_855085, whole genome shotgun sequence.
ACCESSION   DASRJB010000028 DASRJB010000000
VERSION     DASRJB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855085"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFB80_00740"
     CDS             <1..331
                     /locus_tag="VFB80_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32303.1"
                     /translation="RNIFDLALVAAVMKSHDLPGQVGWHMTHFGPQGDYQPELGVAPK
                     EVESVINRRLIGGKHVVAAVSGGVTADPRSLAAREAVTIGNSGQMATYRDNSRPHQLP
                     RRAWWWD"
     gene            402..938
                     /locus_tag="VFB80_00745"
     CDS             402..938
                     /locus_tag="VFB80_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32304.1"
                     /translation="MKRVALFSVSALVIGAIALFATFQSLSTCSLCGCRGLSIEFQLP
                     FTDVTYCRIQNVQQTPLSQVGKQLALVSAHRHDWKLIHGSGNGIMCAIGRGADLDRNA
                     RSTQVASFVADTARYRDRVEAARWFKTALDARNSKAVFDWLLDYPEHDMGTSQDYEKW
                     RARADHDWPEFVERNRRP"
     gene            complement(935..1147)
                     /locus_tag="VFB80_00750"
     CDS             complement(935..1147)
                     /locus_tag="VFB80_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32305.1"
                     /translation="MNMETIREWLNRAPFEPFVLRMSNGEIHEVRHPENVALGKNRII
                     VVDPVADRAVHLSLIHVNSIEALQAA"
     gene            complement(1203..3788)
                     /locus_tag="VFB80_00755"
     CDS             complement(1203..3788)
                     /locus_tag="VFB80_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZN32306.1"
                     /translation="MSENRTRREFLHESAAVAATALAANAAVAAPAEKSTDFASRWEQ
                     SFDRVWLGPEYWANPLQDWHIRDGRIECINAAINRNVHLLTRDLGEQAGELRMSVTIG
                     RLGGGAVGDGQGSAGFRIGIRGPLSEYRNNLIFGQGLDAGFTAGGQLFIGNPASGVPV
                     DLKSESVELRLSVEPLGEMSRVALAALDKAGKELARVSRDDVATNSLAGNLALVANFG
                     AQGTSGGAANAAGKAKKKKAAAPPASAGRFWFAAWKVAGSRVQAHDDRAFGPILFNHY
                     TLHGGTLKMTAQMPPLGEEDSQTVSLEIEHEFTSEKVATAQIHPEAWTATFRVPNWQV
                     TRDVRYRVVYELVAQDGSRRTHAWPGTVRRDPAGGRELVVADVSCNIHEAFPNVRYTA
                     NMAALNPDLIAFVGDQFYESSGGYGVQRAPLPAAILDYLRKWYLHGWTWRELTRDRPS
                     ISIPDDHDVYQGNNWGEGGAPQTGTQEQGGYNMPAAWINVVHQTQTSHHPDPWDAAPA
                     KQGISVYSGPLTYGGVSFAILADRQFKSAPEGKVPPTGGRGDHVTDPNFDPKTADLPG
                     LQLLGPGQMKHLREWVRDWRGVQMKAVISQTIFTAMATTHGAEGRLVADYDANGWPQS
                     ERNAALREIRKVFAFHIAGDQHLPAIVHYGIDAHRDAGLAFAGPAVNTGYPRWFEPLK
                     PGENRAEGAAENTGDFRDSFGNPMTVLAVVNPAKQPRQPVLEKLHDKVSGLGLVRFDK
                     QSGRITVECWPFLADPTQPGTQFPGWPQTFAIEANYGRQATAHLPTLTITAEANPLLE
                     VMDEATGELIYARRIPRRGHQPGVFAEGKYTLRLSIPETGKVKELRGVEAMIRHTAVI
                     EVSIS"
BASE COUNT          640 a         1240 c         1276 g          726 t
ORIGIN      
        1 gcgcaacatc ttcgacctgg cgctggtcgc tgcggtgatg aagagccacg acctgcccgg
       61 ccaggtcggc tggcacatga cgcacttcgg cccgcagggc gactatcagc cggaactggg
      121 cgtcgccccc aaggaagtcg aatcggtcat caaccgccgc ctcatcggcg gcaagcacgt
      181 cgtggccgcg gtcagcggcg gtgtgaccgc cgacccgcgc agcttggcag cgcgcgaagc
      241 ggtgacgatc ggcaacagcg gtcagatggc cacttatcgc gacaacagcc ggccgcatca
      301 gttgccgcgg cgggcctggt ggtgggatta agtagtttcc ggcaacacat accggcggca
      361 ttcagccgcc gtgggagcac actactcccc gctgcacgtc catgaagcgt gtcgcactat
      421 tcagcgtgtc ggcgttggtg ataggtgcga ttgcgctttt cgccacattt caatccttgt
      481 cgacttgttc cctctgcgga tgtcgtgggc tttcgatcga gtttcagtta cccttcacag
      541 acgtcactta ttgccgaatt caaaacgttc agcaaacgcc gctgagccaa gtgggtaagc
      601 aattggcgct ggtgtccgct caccgccatg actggaagct cattcatggc agtggaaacg
      661 gcataatgtg cgcgatagga cgcggcgcgg atctggatcg caacgcgcga tcaactcaag
      721 tggcttcctt tgtcgctgat acggctcgct atcgcgatcg tgtcgaggcg gctcgttggt
      781 tcaaaaccgc actggatgca aggaatagca aggccgtgtt tgactggctc ttggattatc
      841 cagaacacga catgggtacg tcccaggact atgaaaaatg gcgcgctcgc gccgaccacg
      901 attggcctga gttcgtcgag cgcaatcgcc ggccctaagc cgcctgaagg gcttcgatgc
      961 tgttgacgtg tatcaaggat agatgcacgg cgcgatcggc gaccggatcg acaacgatga
     1021 tccggttctt tcccagcgcg acattctcag ggtggcggac ttcgtggatt tcgccgttgg
     1081 acatacggag gacaaacggc tcgaagggag cgcggttgag ccactcgcga atcgtttcca
     1141 tgttcatgtc aacaattcta cgttgcggga acatcgaagc caatttggtc cgctgaataa
     1201 cgtcaactga tgctcacctc aatgacagcc gtatgtcgga tcattgcctc cacgccgcgc
     1261 agctctttca ccttgccggt ttccgggatg cttagccgca gcgtgtattt gccttcggca
     1321 aaaacccccg gctgatggcc ccggcgcgga atgcggcggg cgtagatgag ttcgcctgtg
     1381 gcttcgtcca tgacctccag gagcgggttg gcctcggcgg tgatcgtcag cgtcggcagg
     1441 tgcgccgtgg cctgccggcc gtagttggcc tcgatcgcaa acgtctgcgg ccagccgggg
     1501 aactgtgtgc ccggctgcgt cgggtcagcc agaaagggcc agcattcgac ggtgatccgc
     1561 ccgctctgct tgtcgaaccg caccaggccc aggccggaga ctttgtcgtg cagcttctcc
     1621 aagaccggct gccgcggctg cttggccgga ttgacgacgg ccagcaccgt catcggattg
     1681 ccgaacgaat cgcggaagtc gccggtgttc tccgccgccc cctcagcgcg gttctcgccg
     1741 ggcttgagcg gttcgaacca acggggatag cccgtgttga cggcggggcc ggcgaaggcg
     1801 agcccggcgt cgcgatgggc atcgatgcca tagtgcacga tcgccggcag atgctggtcg
     1861 ccggcaatgt ggaaggcaaa caccttgcgg atttcgcgga gggcggcgtt gcgttcggac
     1921 tgcggccagc cgttggcgtc gtaatcggcg accaggcggc cctcggcgcc atgcgtggtg
     1981 gccatcgcgg tgaagatcgt ctgcgagatg acagccttca tctgcacacc gcgccagtcg
     2041 cggacccact cgcgaagatg cttcatctgg cccgggccga gcaactgcag cccgggcagg
     2101 tccgccgtct tcggatcgaa gttcggatcg gtgacgtgat cgccgcggcc gccggtcggc
     2161 gggactttgc cctcgggggc gctcttgaac tgccggtcgg cgaggatcgc aaagctgacg
     2221 ccgccatagg tgagcggccc ggaatagacg ctgatgccct gcttcgcggg cgcggcgtcc
     2281 cagggatcgg gatggtgcga ggtctgcgtc tggtggacga cgttgatcca ggccgcgggc
     2341 atgttgtagc ccccctgctc ctgcgtgccg gtctgcggcg cgccgccctc gccccagttg
     2401 ttgccctgat agacgtcgtg gtcgtcggga atcgagatac tcggccggtc gcgggtcagc
     2461 tcgcgccacg tccagccgtg gagataccac ttccgcaggt aatcgaggat tgcggctggg
     2521 agcggcgctc gctgcacgcc gtagcctccc gagctttcgt agaactggtc gccgacgaag
     2581 gcgatcaggt ccgggttcag cgcagccatg ttggccgtgt accgcacgtt cggaaacgcc
     2641 tcgtgaatgt tgcacgagac gtcggccacg accagctcgc ggccgccagc cggatcgcgg
     2701 cggacggtgc ccggccaggc gtgcgtccgg cggctcccat cctgcgcgac cagctcatac
     2761 acgacgcggt agcggacgtc gcgcgtgacc tgccagttcg gcacgcggaa cgtcgccgtc
     2821 caggcttccg ggtggatctg ggctgtcgcc actttctccg aggtgaactc atgctcgatc
     2881 tcgaggctga cggtctgcga atcctcctcg cccagcggcg gcatctgggc cgtcatcttt
     2941 agcgtgccgc cgtgcagcgt gtagtggttg aacaggattg ggccgaaggc gcggtcgtcg
     3001 tgggcttgca cgcggctgcc ggcaactttc cacgcggcaa accagaaccg gcccgcgctg
     3061 gccggcggag cggccgcttt tttcttcttt gccttgccgg cggcatttgc tgcaccgccg
     3121 ctggtgccct gagcgccgaa gttggcgacc agcgccaagt tgccagccaa cgagttggtg
     3181 gcaacgtcgt cgcgcgaaac tcgggccagc tccttgccgg ccttgtcgag cgccgccagc
     3241 gctacgcggc tcatctcgcc cagcggctcg accgacagcc gcagttcgac gctctcggac
     3301 ttgagatcga ccggcactcc gctggccgga ttgccgatga acagctggcc gcccgccgtg
     3361 aagccggcgt cgagaccctg gccgaagatc aggttgttgc ggtactcgct cagcgggccg
     3421 cggatgccga tccggaatcc agctgacccc tggccatcgc caacggcgcc gccacccagc
     3481 cggccgatcg tcacgctcat ccgcaactcg ccggcctgct cgcccaggtc gcgggtcagc
     3541 aaatgcacgt tgcggttgat cgcggcgtta atgcactcga tccgcccgtc acggatgtgc
     3601 caatcctgga gcggattggc ccagtactcc gggccgagcc agacgcggtc gaacgactgc
     3661 tcccagcggc tggcaaaatc ggtggacttt tccgccggcg ctgcgacagc cgcgttagcg
     3721 gcgagcgccg tcgcggccac tgccgccgac tcgtgcagga actcgcggcg cgtacgattc
     3781 tcggacatgg ttgagcctcc ggcacattgt cgatagacca aatgtatcgg tggattccat
     3841 tgccagggcg tggagagcgg cgccgacggt cggctgctgg cc
//
LOCUS       DASRJB010000029         7530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1136757, whole genome shotgun sequence.
ACCESSION   DASRJB010000029 DASRJB010000000
VERSION     DASRJB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136757"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFB80_00760"
     CDS             complement(<1..806)
                     /locus_tag="VFB80_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32307.1"
                     /translation="MTAALGVRAASGQYPPPPIPQQPQYQQPQYQQPPQYAQQPQQAA
                     YNLPPASPPVEVFKPAEIMARIGDKCIFYGDVGPTIDLMLDPQLSRIKQELLKKAAEQ
                     RLDPAETQEKLAALEREFLRQGRTVYAKPVLEQLVTSKMLYLEFDRQIRKNAKDKYAE
                     ARAGIDGKLRSHFEKQLEMCREFVPKAKPEELTALMNQVPVAAPLAALMHNHQLESMS
                     ELDAKLRTMGTSLEQQIVQFSEGTLGRETVNRNIDQKPTVTHQEMLDYYE"
     gene            complement(906..4484)
                     /gene="mfd"
                     /locus_tag="VFB80_00765"
     CDS             complement(906..4484)
                     /gene="mfd"
                     /locus_tag="VFB80_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZN32308.1"
                     /translation="MTAASPHAAATERMRQLPDVLQEYAGFAEAVRTLTSGRPASFDG
                     VWGSGCALVAAGLARQSRGPLLVVLPTDRAADDAAADLELFIGEEPSFFPAWETAPDE
                     RLVHDETFGERLRVLKALVRSRERPPWRSPAAATTAAIEKPRTGLSRFIKARGGETGV
                     TQTPESSEPPGTPQREFPTAPILVTSIQALLQPSPPKATIAAATRVLRKGERIEIEEL
                     LRWLIERGFHATSAVELPGEFSHRGGILDIFAPDWVRPVRIELFDDEIESLRSFEVST
                     QRSHESLEEIEVTVLPMASGKRELPDNNALQESPNDREADASRSPGHLTDYLPPDAWI
                     LLVDPDKLSEEGKNYLQRVEKPVDFHSVQAVLAECGKFAVATTAEIQQGTAGTHCHLP
                     FESVERFSGDIGRVRDELDTIAHGDEVFVISPTEAEVERLREILGATKLAAAGRLHFP
                     IGTLHAGFRLRPSSPPLRGGTTRDGVAAYGLLLLTASELFHRGELRRLPRRRLGKAID
                     SFLDLREGDLVVHLAHGIGRYRGLRMIEKQGQREEHLEIEFDGGTKIFVPAIKIDLVQ
                     KYVGGTKTRPTLAKVGGKTWLKQKQAAEGAVHDLAVEMLEMQALRMARPGIAFNPDSE
                     WQREFDSSFPYRETPDQLTAIAAIKADMHRPRPMDRLLCGDVGFGKTEVAMRAAFKAV
                     DSGYQVAVLVPTTILAEQHFHSFHERMAEFPFDIAKLSRFCTPEEEREALAGLKSGKV
                     DIVVGTHRLASKDVEFHNLGLVVIDEEQRFGVDVKERLKALRTMVDVLTLSATPIPRT
                     LHMSLVGLRDIANLETPPEDRLAVETKVSRWDDQLLRHAIQRELARGGQIYFVHNRVS
                     DIELIASRLRTLVPDIKLRIGHAQMPDDELEEVMVQFVEHKFDLLLSTTIVESGLDIP
                     NANTIFIDEADRYGLADLHQLRGRVGRYKNRAYCYLIVEQHKHLTPNAARRLRAIEEF
                     DELGAGFAIAMRDLEIRGAGNLLGTQQSGHIAAVGYELYCQLLENAVHILQKRPPKVR
                     LEVEIALPGDAYLPPDYVDDMRLKIDLYRRLTRVSRFEELDDFRSELIDRFGEPPRPV
                     ERLLELTGLKIDAATWRISAIYIENNFVVFKYDDGPRIEQLARLHKGKLRIVDEKSVY
                     LPLPTDLTETDRIWRIVKSVLRAPPAATTIPTARAGGHALRGVPGR"
     gene            4593..5501
                     /locus_tag="VFB80_00770"
     CDS             4593..5501
                     /locus_tag="VFB80_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZN32309.1"
                     /translation="MQALVTGATGFVGKRLLAKLQRPVVLSRDATKAAKKLEKLGANV
                     YAWEPQGGSPPAAAFEGIDTVIHLAGDPVAEGRWTAAKKARIRDSRVIGTRNLVAGMR
                     NLPVKPKVLVSASAVGIYGSRGEEELTESSSPASDFLAEVCQGWEREALAARELGIRV
                     VPVRVGIVLGEKGGALGKMLTPFKLGLGSPLGSGNQYMPWVHIDDLVEMMLFAAREPS
                     VTTPLNGVAPHPVTNREFTKTLGRVLGRPTFFPPVPGFMLKLMFGEFGQVLLASQKVL
                     PRAALAAGFPFKFAELEPALRDVLHR"
     gene            5565..6062
                     /locus_tag="VFB80_00775"
     CDS             5565..6062
                     /locus_tag="VFB80_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN32310.1"
                     /translation="MPRTYSFERTQLIRRPLAETFAFFAEPANLERITPEFLGFQILT
                     PQPVAMRAGTLIDYKLRLFGMPLGWRTLIDSFEPPFRFSDTQVRGPYRLWHHEHEFSE
                     APEGTWMVDRVWYQLPLGQLGRLAHGLFVGRTLKRIFDHRYQAIQRLLSPTAAALPET
                     PAGRW"
     gene            6148..6990
                     /locus_tag="VFB80_00780"
     CDS             6148..6990
                     /locus_tag="VFB80_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN32311.1"
                     /translation="MSSAQLGTLVTYNPWTIGATTLLADVTARFDELGIHHVAVVDSE
                     RHVIGILSESDLLRSRQAERTLAAVGGGPDTDDAPAVFARDVMSRQVVSVPLTATAAD
                     ALRVLLDKQIHALPVVQDRRLIGMISSRDFLREFSYGEVPASHEPIASLLGEPGPEPL
                     DPDATLDEAFLAMHESGVSRLAVAKDGCPVGILTQRDIIRERCRQMENEGGGLNSQPP
                     SDTIHSAVRSSPILRPGHRLCDAAAAMVDQRLPAVTIVNPSNRLLGLVTEDDILRVLY
                     DAQA"
     gene            6987..>7530
                     /locus_tag="VFB80_00785"
     CDS             6987..>7530
                     /locus_tag="VFB80_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN32312.1"
                     /translation="MKAWIDLNHRIVACSLCPRLRDHCRQVAADKRRAFAQWEYWGRP
                     VQNFGTAKAGLLIVGLAPAAHGANRTGRMFTGDRSGEWLYRALHRAGFANQAESSDPR
                     DGLKLIDCAITAVCHCAPPDNKPLPAEIANCRQWLIGTIDLVQPRVQVALGQIAWKAL
                     LAEARRRNWHAGALPKFGHAA"
BASE COUNT         1220 a         2532 c         2442 g         1336 t
ORIGIN      
        1 tgctcgtagt agtcgagcat ctcctggtgg gtcacggtcg gcttctggtc gatgttcctg
       61 ttcaccgtct cgcggcctaa ggtcccctcg ctgaactgca cgatctgctg ctcgagcgag
      121 gtgcccatgg tgcggagctt cgcgtcgagc tcgctcatcg attccagctg gtggttgtgc
      181 atcagcgcgg cgagcggggc cgccacggga acctgattca tcagggcggt gagctcctcg
      241 ggcttggcct tcggcacgaa ctcgcggcac atctcgagct gtttttcgaa atgtgaccgc
      301 agcttgccgt cgatcccggc ccgggcctcg gcgtacttgt ccttggcgtt cttgcggatt
      361 tgccggtcga actccaggta gagcatcttc gacgtcacga gctgttccag caccggcttg
      421 gcgtacactg tgcggccctg ccgcagaaac tcccgctcca gcgcggccag cttctcttgc
      481 gtttccgctg gatcgagccg ctgctcggcc gccttcttca gcagctcctg cttaattcgc
      541 gacagctgcg ggtcgagcat caggtcgatg gtcggtccca cgtcgccgta gaaaatgcac
      601 ttgtccccga tgcgggccat tatctccgcc ggcttgaaga cctcaaccgg aggcgacgcg
      661 ggcggcaagt tgtacgccgc ctgctgcggc tgctgcgcat actgcggcgg ctgctgatac
      721 tgcggctgct ggtattgcgg ctgctgcggg atcggcggcg gaggatattg cccgctcgcc
      781 gcgcgcacgc cgagcgcagc ggtcacgacc aacgccaagc gcgcagctcg cagactcaca
      841 acgtttggct ttcagcaaat ctctgtaggg aacgccttca gtggcgctcc tgcgggcgaa
      901 agaccctagc gtccgggcac gccacggagg gcgtgccctc cagcgcgggc ggtcgggata
      961 gtagtggcgg ccggcggggc tcgcaacacc gatttgacga tccgccagat gcggtcggtc
     1021 tctgtaaggt cggtcggcag cggaagatat acgctcttct catcgacgat ccgcagcttc
     1081 cccttgtgca gacgggccag ctgctcgatc cgcggcccgt cgtcgtattt gaagaccacg
     1141 aagttgttct cgatgtaaat cgccgaaatt cgccaggttg ccgcgtcgat cttcaggccg
     1201 gtcaactcca gcagccgctc caccggccgc gggggctcgc caaaccggtc gatcagctcg
     1261 ctgcgaaagt cgtccagctc ttcgaaccgg ctgacgcgag tcagccggcg ataaaggtcg
     1321 atcttcagcc gcatgtcgtc cacatagtcg ggcggcaggt aagcgtcgcc tggcagggcg
     1381 atctcgactt ccaggcggac tttcggcggc cgcttctgga gaatgtggac ggcgttctcc
     1441 agcagctggc agtagagctc gtagccgacg gcggcgatgt gcccgctctg ctgggtgccg
     1501 agcaggttgc cggccccgcg gatttccagg tcgcgcatcg cgatagcgaa cccggcgccg
     1561 agctcgtcga actcctcgat ggcccgcagg cggcgggcgg cgttgggtgt gaggtgcttg
     1621 tgctgctcga cgatcaggta gcagtacgcc cggttcttgt agcggccgac gcggccccgc
     1681 agctggtgca ggtcggccag gccgtaacgg tcggcctcat cgatgaagat cgtgttcgcg
     1741 ttggggatgt cgaggccgct ctccacgatc gtcgtcgaga gcaagaggtc gaacttgtgc
     1801 tcgacaaact ggaccatcac ctcttccagc tcgtcgtcgg gcatctgggc gtggccgatt
     1861 cgcagcttga tatcgggcac gagcgttcgc aggcggctag cgatcaattc gatgtcgctg
     1921 acgcgattgt gcacgaagta aatctgccca ccgcgggcca gctcgcgctg gatcgcgtgg
     1981 cgcaggagct ggtcgtccca gcgggagact ttcgtctcca cagccaggcg gtcttccggc
     2041 ggcgtttcca gattcgcgat gtcccgcagg ccgaccagcg acatgtgcag cgtccgcggg
     2101 atcggcgtcg ccgaaagcgt gagcacatcg accatcgtcc gcagggcctt cagccgctct
     2161 ttcacatcga cgccgaaccg ctgctcctcg tcgatgacga ccaggcccag gttgtggaac
     2221 tcgacgtcct tcgacgccag gcggtgcgtg ccgacgacga tatcaacctt gcccgacttc
     2281 aggcccgcga gcgcctcgcg ctcttcctcc ggggtgcaga accggctgag cttggcgatg
     2341 tcgaacggga attcggccat ccgctcgtgg aagctgtgaa agtgctgttc ggcgaggatc
     2401 gtcgtgggca cgagcaccgc gacctgatag ccgctgtcga ccgccttgaa cgcggcccgc
     2461 atggcgactt ccgtcttgcc gaagccgacg tcgccgcaaa ggaggcgatc catcggccgc
     2521 gggcggtgca tgtcggcctt gatggcggcg atggcggtga gctggtcggg cgtctcacgg
     2581 taagggaacg acgagtcgaa ctcgcgctgc cactcgctgt cgggattgaa ggcgatgccg
     2641 ggccgggcca tgcggagggc ctgcatttcg agcatttcga cggccaggtc gtggaccgcg
     2701 ccttcggcgg cctgcttctg cttgagccac gttttcccgc cgaccttggc cagcgtcggc
     2761 cgcgtcttgg tgccgccgac gtatttctgg acgaggtcga tcttgatcgc cggcacgaag
     2821 atcttggtgc cgccgtcgaa ctcgatctcc aggtgctcct ctcgctgccc ctgtttctcg
     2881 atcatccgca ggccgcgata ccggccaatg ccgtgcgcca ggtggacgac caggtccccc
     2941 tcgcgcaagt cgaggaacga gtcgatcgcc tttcccagcc gccgccgcgg cagccgtcgc
     3001 aattccccgc ggtggaagag ctcggaggcg gtgagcagga ggagaccgta ggccgcgact
     3061 ccgtcgcggg ttgtcccgcc acggagtggc gggctactcg gccgcaggcg gaagcccgcg
     3121 tgcagcgtgc cgatgggaaa gtgcagccgg ccggcggcgg cgagcttcgt ggcccccaaa
     3181 atctcgcgga gcctttcgac ttcggcctcg gtaggcgaga tgacgaaaac ctcgtcgccg
     3241 tgcgcgatcg tatccagctc gtcgcggacc cggccgatgt cgccgctgaa ccgctcaacc
     3301 gattcaaacg gcaagtggca gtgcgtgccg gccgtgcctt gctgaatctc ggcggttgtt
     3361 gcgacggcga acttgccgca ttcagccagc accgcctgga ccgaatggaa atccaccggc
     3421 ttctcgactc gctgcagata gttcttgccc tcttcgctca acttgtcggg atcgacgagc
     3481 agaatccacg cgtccggcgg caggtagtca gtcagatggc ccggcgagcg ggaggcgtca
     3541 gcctcccgat cattcggcga ctcttgtaac gcattgttat caggaagctc acgcttcccg
     3601 ctcgccattg gcaacaccgt cacctcaatc tcctcgagag actcgtggct ccgctgcgtg
     3661 ctgacttcaa acgaccgcag cgactcgatc tcgtcgtcga acaactcgat ccgcaccggc
     3721 cgcacccagt ccggcgcgaa gatgtcgagg atgccgccgc ggtgcgaaaa ctcgccgggc
     3781 aattcgaccg cactcgtcgc atgaaatccg cgctcgatca gccaccgcag cagctcctcg
     3841 atctcgatcc gctcgccttt tcgcagcacg cgcgtcgccg ccgcgatcgt cgccttcggc
     3901 ggcgaaggct gaaggagcgc ctgaatggac gtcaccaaaa tcggcgctgt agggaactcc
     3961 ctctgtggcg ttcccggcgg ttcggacgac tctggcgttt gggtgacacc cgtctcgccg
     4021 ccgcgggcct taatgaaccg cgacaaccca gtccgcggct tttctatggc cgcagttgtc
     4081 gcggccgccg gggaacgcca cggagggcgt tccctactcc gcaccaacgc cttcagcacc
     4141 cgcagccgct cgccaaaggt ctcgtcgtgg accagccgct cgtcgggagc ggtttcccag
     4201 gcgggaaaga acgagggctc ttcgccgata aagagttcca ggtcggccgc ggcgtcgtcc
     4261 gccgcccggt cggtcggcag aaccaccagc agcggtccgc gagattgccg ggcgagccct
     4321 gcagccacca gcgcgcagcc tgagccccag actccgtcga atgaggcggg tcgcccggac
     4381 gtgagtgtcc ggaccgcctc ggcgaagccc gcatattcct gcaaaacgtc tggtagctgc
     4441 cgcatccgct cggtcgcggc tgcgtgcggc gatgctgccg tcatggcagg ggaattctag
     4501 cggccagccg gccgcgggcg agaggccaaa atcacgagtc tggttaaaca ggctatgctg
     4561 ttgcggtttg gcgaaagcga gagaggatct ccatgcaagc actcgtcacc ggcgcgacgg
     4621 gattcgtggg caaacgcctg ctggcgaagc tgcagcggcc ggtcgtgctc agtcgcgacg
     4681 cgacgaaggc tgccaagaag ctggagaaac tcggggccaa cgtctatgcc tgggagccac
     4741 agggcggctc gccgccggcc gcggccttcg agggcatcga cacggtcatt cacctggcgg
     4801 gcgacccggt cgccgaggga cggtggaccg ccgcaaagaa agccaggatt cgcgacagcc
     4861 gcgtgatcgg cacgcggaac cttgtcgcgg gaatgcgcaa cctcccggtg aagcccaaag
     4921 tcctggtcag cgcatcggcc gtcggcatct acggctcgcg gggcgaagag gagctgaccg
     4981 agtcgagctc gccggcgagc gactttctgg ccgaggtgtg ccagggctgg gagcgcgagg
     5041 cactggccgc ccgcgagctg ggaatccgcg tcgtgccggt ccgcgtcggc atcgtgctcg
     5101 gcgaaaaggg cggggcactc ggcaaaatgc tgacgccgtt caagctcggc ctgggcagcc
     5161 cgctcggcag cggcaaccaa tacatgccct gggtgcatat cgacgacctc gtagaaatga
     5221 tgctgtttgc cgcccgcgag ccgtccgtga cgacgccgct caacggcgtg gccccgcatc
     5281 cggtgaccaa ccgcgaattc accaaaacac tcggccgcgt cctgggccgg cccacgttct
     5341 tcccgcccgt gcccggcttc atgctcaagc tgatgttcgg cgaattcggc caggtgctgc
     5401 tcgcgtcgca gaaggtcctg ccgcgggccg cactcgccgc cggctttccg ttcaagttcg
     5461 ccgagctcga gccggctctg cgcgacgtgt tgcatcggta accatcgcga ccactggtcg
     5521 cggagcccgg caaaccgcga ccactggtcg cggctttgag acgcatgccc cgtacctaca
     5581 gcttcgagcg cacgcagctc atacggcggc cgctggctga aacgttcgcg ttcttcgccg
     5641 aaccggcaaa cctcgagcgg atcacgcccg agttcctcgg ctttcagatc ctcacgccgc
     5701 agcccgtcgc aatgcgggcc ggaacgctga tcgactacaa gctgcggctg tttggcatgc
     5761 cgctgggctg gcgaacgctg atcgactcgt tcgagccgcc tttccgcttc agcgatacgc
     5821 aagttcgcgg cccttatcgg ctgtggcatc acgaacacga attcagtgaa gcacccgaag
     5881 gcacctggat ggtcgatcgc gtgtggtacc agctgccgct ggggcaactg ggacgcctgg
     5941 cccacggctt gttcgtcggc cggacgctca agcggatttt tgaccatcgg tatcaggcga
     6001 tccagcggct gctctccccc acagcggcgg cattgcccga aacgccagcc ggccgctggt
     6061 gagctacatt tttctgggcg ctttgcgcac catgaggtcg ggctgtaact gcccggccga
     6121 ttttgccgcc gttgcggaga tttccggatg tcgagcgcac aactgggtac gctggtcacc
     6181 tacaacccgt ggacgatcgg ggccacgacg ctgctggccg acgtcaccgc gcgcttcgac
     6241 gagctgggca tccatcatgt agcggtcgtc gatagcgagc ggcatgtgat cggcatcctg
     6301 tcggagtcgg acttgctgcg ctcccgccag gccgaacgga cgctggccgc agtcggcggc
     6361 ggcccggaca ccgacgacgc tccggccgtc ttcgcccgtg atgtgatgtc ccgccaggtc
     6421 gtctcggtcc cgctcacggc caccgcggcc gacgcgctgc gggttctgct cgacaaacaa
     6481 attcacgccc tgcccgtcgt gcaggaccgc cgtctgatcg gcatgatcag cagccgcgat
     6541 ttcctgcgcg agttctccta cggcgaagtg cccgcctcgc acgagccaat cgcgtcgctg
     6601 ctgggtgagc ccggcccgga gccgctcgat cccgacgcca cactcgacga agccttcctg
     6661 gcgatgcacg aatcgggcgt cagccgcttg gccgtggcca aggacggctg cccggtgggc
     6721 atcctgacgc agcgcgacat cattcgcgag cgctgccggc agatggagaa tgaagggggc
     6781 ggcctgaact cccagccgcc aagcgacacc atccactcgg ccgtccgcag ctcgcccatt
     6841 ctgcggcccg gtcatcggct gtgcgatgcc gcggccgcca tggtcgatca gcggctcccc
     6901 gcggtcacga tcgtcaaccc gtcgaaccgt ctgctgggcc tggtcaccga ggacgacatc
     6961 ctgcgcgtcc tgtacgatgc ccaggcatga aggcctggat cgatctcaac caccgaatcg
     7021 tcgcctgttc actctgcccg cggctgcgcg accattgccg gcaagtcgcg gccgacaagc
     7081 ggcgggcgtt tgcccagtgg gaatactggg gccggcccgt gcagaatttc ggcactgcca
     7141 aggccggtct gctaatcgtc ggactcgcgc cggcggctca cggagccaac cgcaccggcc
     7201 ggatgtttac cggcgaccgt agcggcgagt ggctctaccg ggcgctccac cgagccggct
     7261 tcgccaacca ggccgagtcg tccgatccac gcgacgggtt gaaactgatc gactgtgcga
     7321 ttaccgccgt ctgccactgt gcgccgcccg acaacaagcc gctgccggcg gaaatcgcca
     7381 actgccgtca atggctaatc gggacgatcg acctcgtgca gccgcgggta caggttgccc
     7441 tcggccagat tgcctggaaa gcactactgg ccgaagcccg ccgccgcaat tggcacgccg
     7501 gcgcgctgcc gaaattcggc cacgccgccc
//
LOCUS       DASRJB010000030         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_857889, whole genome shotgun sequence.
ACCESSION   DASRJB010000030 DASRJB010000000
VERSION     DASRJB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857889"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFB80_00790"
     CDS             <1..396
                     /locus_tag="VFB80_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-6-sulfatase"
                     /protein_id="HZN32313.1"
                     /translation="WPGRIPSGRVSDEFLTTLELLPTLASAAGMRPAENLPLDGFDML
                     STLAGKSPSPRKEMYWEFRGQKAARVGNNKWIEANNYRGLFDLTSDLGEKIDLTEKQP
                     ALAAEIAGKWAAWRKEMEEAEPRGPFRDY"
     gene            399..1304
                     /locus_tag="VFB80_00795"
     CDS             399..1304
                     /locus_tag="VFB80_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN32314.1"
                     /translation="MRLLVACRKCKLRYDATGRNVGQRFRCRCGNVVTIQRPKGHDAS
                     VVHCSTCGAAREEGSKACGYCGSDFTIHETDLETICPGCLARVSDRARYCHHCATPLA
                     ADVVAGEASKLICPVCPGRKLFSRRLPDAAVTVLECRVCAGMWIGLEAFHDLLNAEER
                     RKERLTVTHRPAAAAKQQGYRKCPDCAGLMIRRNLGRGKSGVVLDVCGEHGLWFDCDE
                     LSHALAWIRAGGLEDARQCVARLKRSPDVHRKRQMQETAADARPAGQPAAEGVFIEAH
                     RRGILVNLGLMTAALSAMFDLSQGD"
     gene            1361..2812
                     /locus_tag="VFB80_00800"
     CDS             1361..2812
                     /locus_tag="VFB80_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN32315.1"
                     /translation="MRWLWLSFCLALLTWCSSYLRVSSAADRPNILWLIAEDFSPDLG
                     CYGTSEVSTPNLDKLAGQGVRYTRFFTTAPVCSASRSAFMTGMYQTTIGAHNHRSHRD
                     DGYQLPDGVRVLTDWLRDASYFTANVRELPAEFGFRGAGKTDWNFTYTGQPFDSDKWS
                     DLAGHQPFYAQVNFQETHRAFRAPPHADPAKVVIPPYYPDHPVTRTDWAQYLDAATEL
                     DRKIGLILSQLEKDGLSDSTIVCFLGDHGQCHVRGKQFCYDSGLRVPLIIRWPKNFAP
                     PAHFKAGSVDDRLLASIDLSATTLALAGAKKPAKMQGEIFLGDTASPPRKYVFGARDR
                     CDETVFRFRTVRTERYRYIHNFTPERPFLQPNDYKERSYPVWNLLKQLDAEGKLTPLQ
                     KVLTAPSMPPEELYDVVADPYETKNLAGSPEHAAVLQELRGVVDRWIEESNDQGKQLE
                     PPELAAAEGRTKPLPPDAAEKKAKKQKAAKGAK"
BASE COUNT          547 a          952 c          955 g          484 t
ORIGIN      
        1 tggccgggca ggattccctc cggccgcgtc agcgacgaat ttctgaccac gctcgaactg
       61 ttgccgacgc tggcctctgc tgcgggcatg cggccggccg agaaccttcc gctggatggg
      121 tttgacatgc tctcgacgct cgccggcaaa tcgccttcac cgcggaagga gatgtattgg
      181 gaattccgcg gccagaaggc ggcccgcgtc ggcaataaca agtggatcga ggcgaacaac
      241 tatcgcgggc tgtttgacct gacgagcgat ctcggcgaga agatcgacct gacagagaag
      301 cagccggctc tcgccgccga aatcgccggc aagtgggcgg cctggcgcaa ggagatggaa
      361 gaagcagagc cgcgggggcc gtttcgggac tactaaccat gcgtctgctc gttgcctgcc
      421 gcaagtgcaa attgcgatac gacgccaccg gccgcaacgt cggccagcgg ttccgctgcc
      481 gctgcggcaa cgtcgtaacg atccagcgcc ccaagggcca tgacgcctcc gtcgtccatt
      541 gctcgacgtg cggcgcagcg cgcgaggaag gctcgaaggc ctgcggctac tgcggctccg
      601 actttacgat ccacgaaacc gacctggaga cgatctgccc cggctgcctg gcccgcgtga
      661 gcgatcgggc ccgctattgc caccactgcg cgacgccgct ggctgccgac gtggttgccg
      721 gcgaggccag caagctgatt tgtcccgtct gccccggcag gaagctgttc agccggaggc
      781 tgcccgatgc ggcggtaacg gtcctggagt gccgcgtctg cgccggcatg tggatcggcc
      841 tggaggcgtt tcacgacctg ctcaatgccg aagagcggcg gaaggagcgg ctgacggtga
      901 cgcatcgtcc cgcagcggcc gcgaaacagc agggctaccg caagtgcccg gattgcgcgg
      961 ggctgatgat ccgccgcaac ctgggacggg gcaaaagcgg cgtcgtgctg gacgtgtgcg
     1021 gcgagcacgg cctgtggttc gactgcgacg agctgtctca cgccctggcc tggattcgcg
     1081 ccggcggatt ggaggacgcc cgccaatgcg tggccaggct caaacgctcg ccggacgtcc
     1141 accgcaagcg gcaaatgcag gagacggcag ccgacgcgcg gcccgcagga caaccggctg
     1201 ccgaaggcgt gttcatcgag gcgcaccggc ggggaatctt agtcaacctc ggattgatga
     1261 cggcggcgct atcggcgatg tttgatctgt cgcagggcga ttgagtgccg cattcaattg
     1321 acacgaaggc acgaaggtca ccaagcaagg gagggttgca atgagatggc tgtggctttc
     1381 gttctgcctt gccttactta cttggtgctc ctcgtacctt cgtgtttcgt ccgccgctga
     1441 ccgcccgaac attctctggc tgatcgccga agacttcagc cccgatctgg gctgttatgg
     1501 cacgtcggaa gtctcgacgc cgaacctcga caagctggcg gggcagggcg tgcggtacac
     1561 gcggtttttc acgaccgcgc cggtttgttc ggccagccgc agcgccttca tgacgggcat
     1621 gtaccagacg accattggcg cccacaacca tcgctcgcat cgcgacgacg gctaccagtt
     1681 gcccgacggg gtgcgcgtgc tcaccgactg gctgcgcgac gccagctatt tcacggccaa
     1741 cgtccgtgag ctgccggctg agttcggctt tcgcggggca ggcaagaccg actggaactt
     1801 cacctacacg ggccagccgt tcgacagcga caagtggagc gacctggcgg ggcatcagcc
     1861 gttctatgcc caggtgaact ttcaggagac gcaccgggcg ttccgtgctc cgccgcacgc
     1921 cgacccggcg aaggtcgtga ttccgccgta ttaccccgat cacccggtga cgcggactga
     1981 ttgggcccag tatctcgacg cggcgactga gttggaccgc aagatcggcc tgatcctctc
     2041 ccagctcgag aaggacggcc tgtctgacag caccatcgtc tgcttcttgg gcgaccacgg
     2101 ccaatgccac gttcgcggca aacaattctg ctacgacagc gggctgcgcg tgccgctgat
     2161 catccgctgg cccaagaatt tcgccccacc ggcgcacttc aaagccggct cggtggatga
     2221 ccggctgctg gcctcgatcg acctgtcggc aacgacgctg gcgttggccg gggcgaagaa
     2281 gcccgcgaag atgcagggag aaatcttcct gggagacacg gccagcccgc cgcggaagta
     2341 cgtcttcggc gctcgcgacc gctgcgacga gacggtcttt cgcttccgca ccgtacgcac
     2401 ggagcgctat cgctacatcc acaactttac gccggagcgg ccgttcctgc agccgaacga
     2461 ttacaaggag cggtcgtacc ctgtgtggaa tctgctcaag cagctcgacg ccgaaggcaa
     2521 gttgacgccg ctgcaaaagg tcctcacagc gccctcgatg ccgcccgaag agctgtacga
     2581 cgtggtggcc gatccctacg agaccaagaa cctggccggc tcgccagagc acgccgccgt
     2641 ccttcaagag ctgcgcggcg tggtcgatcg ctggatcgaa gaatcgaacg atcaaggcaa
     2701 gcaactggag ccgccagagc tcgccgccgc cgaaggccga accaaaccgt tgcctcccga
     2761 cgctgccgaa aagaaggcga agaaacagaa ggcagcaaag ggagcaaagt agatcctaga
     2821 actcgtccag cgcgtcgatc agcgagtcta gctcgtcctt gggcgcagac tgcctggctt
     2881 cgtgccgctt cggcaggccc acgctcgcgc cgacggcctg gcggccggcc agtaagag
//
LOCUS       DASRJB010000031        14425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_10306, whole genome shotgun sequence.
ACCESSION   DASRJB010000031 DASRJB010000000
VERSION     DASRJB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10306"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="VFB80_00805"
     CDS             complement(<1..1997)
                     /locus_tag="VFB80_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN32316.1"
                     /translation="MAKRKPKPQAAGKLAGKRLALVGKFGYRDLVRKQYASQIQSAGG
                     AVVDGAKAAADYVVVGEGRGGKPPGDLAKLQKKWPQIQVLSPADFARLVLPDREELLR
                     EIGKGRRKEEDRFWEGLAWACHESGVPIDLRKADLRKADLYGAHLQGVDLTGSDLRGA
                     TLKYSHFGNLERVNFDDCKGESVYLQNLQQCTFRNAELEDTWMFDADAKRVEACDFTA
                     AKMRNTRFGRGTLVDCKFAGADLSDSELEAAVFERADFTKADLSRVHASTAKFAGCTF
                     AKANLSRADLRGAALAGADLRNANLREAVLSEADLTGANVAGADFQDAVLTGTKLQGL
                     DLSKARNFKPPVARAAGPKLQELAAAAAGAKEFVTSAEIDLGQDEFARLQVSVVGRFL
                     GAHSRYFRDGSDVSDRVSAPTFEQGLLNLADRWPKATLRLDSIRAKGSKTVRGPKLKE
                     LAAAAWAETFGVADLSPQQLQAQRASQHAAALSERDALMKKIRAQGVKAWNGIDFRLR
                     RRIELQGADLSGAKLDKVNMCGYDFKGANFSNSSLIEAELWNSEFPKGVFSGANLERC
                     SFENAVLNGANFQKANLTGANLANSKLLGADFTGAKLKDASFAAAQYDQATIFPKGFT
                     PPSDMTWKGDGPPPNAKKVKAAKAGSLDFDSFLAQLNTKVEV"
     gene            1996..2262
                     /locus_tag="VFB80_00810"
     CDS             1996..2262
                     /locus_tag="VFB80_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32317.1"
                     /translation="MESSRSIKALECVDGKTHTVAEGTRGEKHVGHSRVAARRLLFIP
                     ASSLGEQSMRKALDGQVIQEAALAAIVGVVGAVVMIALTYSMTQ"
     gene            2443..4347
                     /locus_tag="VFB80_00815"
     CDS             2443..4347
                     /locus_tag="VFB80_00815"
                     /inference="COORDINATES: protein motif:HMM:NF026034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HZN32318.1"
                     /translation="MASRIAIFVAVLGWLSALSGARALGESSLPAVVVTEDADSFTLT
                     NGPIAARVSKRSGDLTSLLYQGQEMLESKSGHAGGYWSHDTTGGKETISRITIDPKSN
                     GGERGEVSVKGVSGGIKMGHGPGAAAGGDFPADIEIRYSLGRGDSGIYTYCTFEHLAS
                     YPAATMTEARFCAKLAAMFDWMTLDAKRNQHFPADLREGDKYIYTAVQHEHPVYGWSS
                     MKEHVGFWLINPTVEYLSGGPTKVEFLCHRDTTPVAAACLLNYWRSSHYGGAVVAVGE
                     NEHWTKVIGPFFLYVNSGGGTQELWESAQVQAAKETSKWPYDWVQGVDYPRRAERTSV
                     RGQLKLADAERPGAKTPNLLVGLTHPTYAPPATGARGFGPPRQIDWQTDARHYQFWVR
                     GDEAGNFQIPQVRPGTYTLHAMADGVLGEFTKTDIEVTAGQPLDLGSLAWTPVRKGRQ
                     VWEIGVANRTGREFFQGENYSDPAISLKYATLFPEDVTFTIGKSDYKQDWFFQHIPHN
                     TDAAARVVPFSGIRGSGRATPYTIAFELPEAPRGTATLRVAICGTSARSLEVKVNGQA
                     VGQIERLLNDGAIPRHSIQGLWYERELAFSADLLKQGGNTLQLIVPAGPLNNGVIYDY
                     LRLELAEAAP"
     gene            4366..5472
                     /locus_tag="VFB80_00820"
     CDS             4366..5472
                     /locus_tag="VFB80_00820"
                     /inference="COORDINATES: protein motif:HMM:NF023145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HZN32319.1"
                     /translation="MVNPLRFSLAGLIGVILLVSVYLATLRTSSTSGAGLALSISVGV
                     LTAGLFWGLFRPGADRGFWAGFQLAGWGYLLAAVSGSSQSQLGNHLITTQLVGALYRQ
                     MPVTSTQGVMVEWHGTWYAAEVLRRSGARHYIHYTGYGANWDEWVGPDRIRGQFGPFL
                     QICHALLSLILALAGGAVGGCLGVVNRSRRWFWIPWGGCAAAAVGTSLLAMATDSDLA
                     AGAAWSLLLVLLVLAAIAAWGGPERRSSFALGFAIVGCGYLLLHFGPGLETTVGPNLL
                     STRLLQLVHDWLHPEAAQTVSPAWPYTGTIGSGRVSWAPTYVGIGYSTAVSICVLAGH
                     CLLATLLGLLGGFAALWLSRWRRPDQGRQDAAHA"
     gene            5465..5914
                     /locus_tag="VFB80_00825"
     CDS             5465..5914
                     /locus_tag="VFB80_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN32320.1"
                     /translation="MPESASAGLLLFRRSGGALELFLAHPGGPFWKHKDAGAWTIPKG
                     LIDRGEEPLASAIREFEEETGLPPRGPYLPLGTIRQKAGKTVHAWAFEGDADPANVKS
                     NLVQGSFPEIDRCAWFSPAEARQKLNPAQAAFVDRLEAALREQRQSD"
     gene            6885..7763
                     /locus_tag="VFB80_00830"
     CDS             6885..7763
                     /locus_tag="VFB80_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN32321.1"
                     /translation="MKYKITHTTIYNYTEAVPICHNEVHLTPREHKRQSCLSHRLRIR
                     PEPARIDTRIDAFGNHAGWFSIQEGHERLSVTAHSQVQVAEPDVPAPSTTPPWEEIRD
                     RFALGQPPWWVEARQFVYASPHVPISEQLRAYAQPSFPAGQPWLAGLLDLTRRINQEF
                     HYDPAATTVNTPVAKVLKDRRGVCQDFAHLQIACVRSLGLPARYVSGYLLTTPPPGQP
                     RLVGADASHAWVSAFCPEVGWIDFDPTNNQIPSTRHVTLAWGRDYSDVCPIKGVLVGG
                     GQHRMRVAVDVVPLEA"
     gene            7916..8581
                     /locus_tag="VFB80_00835"
     CDS             7916..8581
                     /locus_tag="VFB80_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32322.1"
                     /translation="MPTRFVFAALALLGLLALTDSASAFGRRWERRRAELYGSLNASL
                     SARVDAGVAQQAAAAEQRLSEANKAQIAAEAAKLNSEVAAALAQLKQQATDAVAAEAR
                     RFQDEAKAQFAALREAAQNELKAEFAKVRDEAKQAVVAEFNRTREELTKQVAAEAQKV
                     KDTMMPELKNAVAAEVKTALAAAKPAEEQPAQKQPEQPADKPAASAATTTTSEKKDAG
                     ESE"
     gene            8597..9340
                     /locus_tag="VFB80_00840"
     CDS             8597..9340
                     /locus_tag="VFB80_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN32323.1"
                     /translation="MPGDYPELDVIAVGAHPDDVEIACGGTLAKLVKQGYRVGIVDLT
                     DGEPTPLSPGPEIRLAEAQQAAATLGARVRVTLELPNRRLFDTFEARVLLAKEFRKYR
                     PKIVIGFGDKTPMASPDHWQAMQITDAAVFYSRLTKWDEHFDGLPVHAIQAQLYFRLA
                     FEAISGGNYPNQFTVDISDTLEQKLASVRCYATQFPPGKAHVFDRIRGAALIAGAASS
                     FAAGEVFISTRPLGTLDLVKTVLPRMQAT"
     gene            9396..10889
                     /gene="trpE"
                     /locus_tag="VFB80_00845"
     CDS             9396..10889
                     /gene="trpE"
                     /locus_tag="VFB80_00845"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZN32324.1"
                     /translation="MPHLPDFDAFRRLADGHQLVPVYRRLLSDALTPVSAFHLLDSGG
                     QACLFESVIGGEKVGRYSFLAVEPFWELIAHGKRVTVRGEGGQRNFESSDPLDELRQR
                     LNAFRAVHLPELPPFEGGAIGYAGYDVVRYVENLPNAPPDDRGLPDLSFAFYDRMVVF
                     DNVSKALFVIAMARLDKQPDARAAYDDACRRVDDLVLRLSQPTNLPPADIATGGTPQV
                     AWKSNLKQEEFENAVRKCVEYIRAGDIFQVVISQRLELELGCDPLELYRTLRVVNPSP
                     FMFFLRTPEVTLVGSSPEIMCRVVDKQATVRPLAGTRPRGATDEEDRKLAEELLADPK
                     ERAEHVMLVDLGRNDIGRVAKFGSVKLSDVMVIERYSHVMHITSNVTGKLRDECDAFD
                     ALKACLPAGTVSGAPKVRAMQIIDELEPHRRGPYAGAVGYIDYAGNMDTCIALRTLVV
                     QGQRAYIQAGAGIVADSVPASEYQETLNKARGLLKAIEVTRQRVKAE"
     gene            10941..11252
                     /locus_tag="VFB80_00850"
     CDS             10941..11252
                     /locus_tag="VFB80_00850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZN32325.1"
                     /translation="MIVVLATIELHPGKRPNFLTEFRQIVPKVRAEAGCIEYFPAIDT
                     ASGLPVQGPAREDVVVVVEKWESLAALETHLIAPHMMEYRPKVKDFVKKVSLQILTPA
                     E"
     gene            11340..12404
                     /locus_tag="VFB80_00855"
     CDS             11340..12404
                     /locus_tag="VFB80_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN32326.1"
                     /translation="MRDLLRHLLAALRSGRPVAWCRLVETRGSTPQKAGAAMLVFGDG
                     SQAGTLGGGCVEAEVKRRALVLVAENRPDVCAFQLDSDYGWDDGLICGGRMQIAVEPI
                     VGNGAAPYFERLSEILERAGGCTEAIVFDRQASGLAAPASFLFDASGAFLAAMRGESP
                     PPQVLDPLRPLSSRPRAYAAHGVAYLPVLPRCTLVIVGGGHVGKAVADLAAELEFDVW
                     IIDDRAEYVAETRFPQVERRISGPIDRVLPELAITGDTYCLIVTRGHSHDQEALFHLA
                     DRGARYVGLIGSRRKIKMIFENLAAEGVSREALAKVHAPLGIDIGSQTVPEIAVSICA
                     ELVAHRNLGKVPGRPRGVEF"
     gene            12406..13029
                     /locus_tag="VFB80_00860"
     CDS             12406..13029
                     /locus_tag="VFB80_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN32327.1"
                     /translation="MARHFAIIPACGQSTRMGRPKLLLPLEGKPLVWHALAAWRASSV
                     ASITAVVRRDDTELAAALAESGADVVLPSLPPADMKASVQAALSHITSRHAPSVDDSF
                     LVAPADIPRLSPAIVDALISRHDEQPGSILVPTLAGRRGHPVLFPWLLSEHVFRLQED
                     EGLNSLASRFPPVEVPCDGLVPAGSRPFADIDTPEQYQALGGEYRDE"
     gene            13211..>14425
                     /locus_tag="VFB80_00865"
     CDS             13211..>14425
                     /locus_tag="VFB80_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32328.1"
                     /translation="MALRSTPPEIRRDPAAARLPLSVVLLVALALSFVALPGCGGCWG
                     GGGANSAAAKKKKEEEEKKKKESKKKKLEKPKDDFEPIAIRMLPSSDPTQNVKNPAIF
                     AKPGHWIALSETLKANNFDFPGELATFAETPADNRPLVVEDTTQRLSIWSPAILPKGQ
                     AKRIESLIYLPRRSGPNLNRTYSLRSELHGVRGGRTEVFANSRNDALKDYQFLIVVLS
                     SNPAAYSQFAKLNSIYMGTVATPDTNELGQDTLRHYHILAPNSDRTVPLPSHPLALTT
                     TSHIFWDDINPAILTALQQEALLDWVHWGGQLVISGPGSLDKLRGSFLAPYLPADSSQ
                     TVKLEQSAFDELNSNYSLQRDPARAGQSPPPPKGEPLRRINVLPERPMLGVELKLHPA
                     AQPIPGTGGLVVE"
BASE COUNT         2551 a         4707 c         4603 g         2564 t
ORIGIN      
        1 acttcgacct tggtattgag ctgcgcgagg aaggaatcga agtcgagcga gcccgccttg
       61 gccgccttca ccttcttcgc attcggcggc ggcccgtcgc ctttccaggt catgtcgctc
      121 ggcggcgtga agcccttggg aaaaatcgtc gcctggtcgt attgcgccgc ggcgaagctg
      181 gcgtccttca gtttcgcgcc cgtgaagtcg gcgccgagga gtttcgaatt ggccaggttc
      241 gcgccggtca aattggcctt ttgaaagttg gcgccgttga ggacggcatt ctcgaaactg
      301 cagcgttcca agttcgcccc cgaaaagact cccttgggaa attcactgtt ccacagctcg
      361 gcttcgatca gcgacgagtt ggagaaattc gcgcccttga agtcatatcc acacatattt
      421 actttgtcga gcttcgcgcc gctcaaatcg gccccctgca attcgatccg ccggcgcagg
      481 cgaaagtcga tcccgttcca ggccttaacc ccctgcgcgc ggatcttctt catcagcgcg
      541 tcccgctcgc tcagcgctgc ggcgtgctgg ctggcgcgct gcgcctgcag ctgctgcggg
      601 ctcaggtcgg cgactccgaa ggtctcggcc caggccgccg ccgccagctc tttcaacttt
      661 ggtccgcgga ccgtcttgct gcctttggcc cgaatgctgt ccagccgcag ggtggctttc
      721 ggccagcggt cggccaggtt gagcagcccc tgctcaaacg tgggcgcgga aacccggtct
      781 gacacatcgc ttccgtcgcg gaaatatctc gaatgcgcac ccaggaagcg gccgacaacc
      841 gagacctgca gccgggcaaa ctcgtcctgg cccaggtcga tctcggccga ggtcacgaat
      901 tccttggccc ccgcggccgc cgcggccagt tcctgcagct tcggccccgc cgcgcgggcc
      961 acaggcggct tgaaattcct ggccttcgac aagtcgagtc cttgcagctt cgtccccgtc
     1021 aataccgcat cctgaaaatc ggcgccggcg acgtttgcgc ccgtgaggtc ggcttcgctg
     1081 agcaccgcct cgcgcaggtt ggcattccgc aagtccgctc ccgcgagcgc ggccccgcgg
     1141 aggtcggcgc ggctcaggtt cgccttcgca aacgtgcaac cggcgaactt cgccgtcgag
     1201 gcatgaacgc ggctcaggtc ggccttggta aagtcggccc gctcgaagac cgccgcttcc
     1261 agctcggagt cgctgaggtc cgcgccggca aacttgcaat cgaccagcgt cccccgtcca
     1321 aaacgggtgt ttctcatttt cgcggccgta aagtcgcacg cttccaccct cttcgcgtcg
     1381 gcatcgaaca tccaagtgtc ttccagctcc gcgttgcgga acgtgcactg ctgaagattc
     1441 tgcaggtaga cgctttcccc cttgcaatcg tcgaagttga ctcgctccag gttgccgaag
     1501 tgcgagtact tcagcgtcgc gccgcgcaag tcgctgcccg tgaggtcaac cccttgcaga
     1561 tgcgcgccgt acaggtccgc cttgcgcagg tcggccttgc ggaggtcgat cggaacgccc
     1621 gactcgtggc acgcccaggc gagtccttcc cagaagcggt cctcttcctt gcgccggccc
     1681 ttgccgatct cgcgcagcag ctcttcacgg tccggcagga ccagccgggc aaagtcggca
     1741 ggacttaaga cttgaatctg gggccatttc ttttgcagct tcgcgagatc gccaggcggc
     1801 ttgccgccgc gcccttcgcc tacgacgaca taatcggcgg ccgccttggc cccatcgacg
     1861 actgcgcctc cggcgctctg gatttgactg gcatattgct ttcgcaccag gtcgcgatag
     1921 ccgaacttgc cgaccagtgc cagccgcttg cccgccagtt tgccggccgc ctgcggcttc
     1981 ggttttcgtt tcgccatgga atcctcacgc agcatcaagg cgcttgaatg cgtcgatggc
     2041 aagacacaca ccgtagcgga gggcacacgc ggggaaaagc atgtcggcca cagccgtgta
     2101 gcggcgcgcc gcttgctctt tattcccgcc tcttctctcg gggaacagag tatgcgaaaa
     2161 gcacttgacg gacaggtcat tcaggaggcc gcgcttgccg caatcgttgg ggtggttggc
     2221 gcggtggtca tgatcgcgct gacgtactcg atgacccaat gacggcagcg tgtcggcttc
     2281 gtcctggcaa cgcgcgggtt gaaaccggcg cttgacggct gcgcaccggt tgcagtcggg
     2341 gctaggccgc gcgcctagct ccgtcaacga gtgcctggca aggcttctgg cggtatcatg
     2401 agcagcaccg tctgggtctc ttatcccctg cgaggtgcgg ccatggcgag tcgaatcgcg
     2461 atctttgtgg cggttcttgg ctggttgtcg gcgctgagcg gcgcgcgggc cttaggggag
     2521 tcgagccttc cggccgtcgt agtcaccgag gatgcggatt cgttcacgct gaccaacggc
     2581 ccgattgcgg cccgggtctc aaagcggtcg ggcgatttga catcgctgct ctaccagggc
     2641 caggagatgc tcgaaagcaa gtcgggccac gccggcggct attggtcgca cgacacgacc
     2701 ggcggaaaag agacgatctc gcgcatcacc atcgacccaa aatccaacgg cggtgagcgg
     2761 ggcgaagtct cggtcaaggg cgtctcgggc ggaatcaaaa tgggccacgg tcccggcgcg
     2821 gctgccggcg gcgattttcc ggccgatatc gagattcgct attcactggg gcgcggcgac
     2881 agcggaatct atacctattg caccttcgaa catctggcca gctatccggc ggcgacgatg
     2941 accgaggccc gcttctgcgc gaagctggcc gcgatgttcg actggatgac gctcgacgcc
     3001 aagcgcaacc agcattttcc cgccgacctg cgcgagggtg acaagtacat ctacactgcc
     3061 gtccagcacg agcatccggt ctacggctgg tcgagtatga aggagcacgt cggtttctgg
     3121 ctcatcaacc cgaccgtcga gtacctgagc ggcgggccga cgaaggtcga gttcctctgc
     3181 caccgcgaca cgacgccggt cgccgcggct tgcctgctga actactggcg gagcagccac
     3241 tacggcggcg cggtcgtggc cgtcggcgag aacgaacact ggaccaaagt gatcggcccg
     3301 tttttcttgt acgtgaattc cggcggcggc acgcaagagt tgtgggagag tgcccaagtt
     3361 caagctgcca aagagacctc caagtggccg tacgactggg tccagggggt cgattatccg
     3421 cgccgcgcgg aacgaaccag cgtccgcggg caactcaagc tggccgacgc cgagcggccc
     3481 ggagcgaaaa ccccgaatct gctcgtcggc ctgacgcatc ccacctacgc gccgcccgcc
     3541 actggtgccc gcgggttcgg tccgccgagg cagatcgatt ggcagaccga cgccagacac
     3601 tatcaattct gggtccgcgg cgatgaagcg ggcaattttc aaatcccgca ggtccgcccc
     3661 ggcacataca ccctgcacgc gatggccgac ggcgtgctgg gcgagtttac caagaccgac
     3721 atcgaagtaa cggccgggca gccgctggat ttgggatcgc tggcgtggac gcccgtgcga
     3781 aagggccggc aagtctggga gatcggcgtt gccaatcgga cgggccgcga gttcttccag
     3841 ggcgagaact actccgatcc ggccatctcg ctgaagtacg cgactctttt ccccgaggac
     3901 gtgaccttca cgatcggcaa gagcgactac aagcaagact ggttctttca acatattccg
     3961 cacaacaccg acgcggcggc ccgcgtcgtg cctttctccg gcattcgcgg cagcggccgg
     4021 gccacgccct acacgatcgc attcgagctg cccgaggccc cgcgcggcac cgcgacgttg
     4081 cgggtcgcga tttgcggcac cagcgcccgc tcgctcgaag tcaaggtgaa cggccaggcg
     4141 gtgggtcaaa tcgagcggct gctgaacgac ggcgcgatcc cgcggcattc gattcaagga
     4201 ctgtggtacg agcgcgagct cgcctttagc gccgatctgc tcaagcaggg cggcaacacc
     4261 ttgcagctca tcgtccccgc cggaccgctc aacaacggag tcatctacga ctacctgcgg
     4321 ctggagctgg ctgaggcagc tccctagcgc gctcgaagcg atccgatggt caatcccttg
     4381 cgattctcgt tggccgggct gatcggagtc atcctgctgg tctcggtcta tctggccacg
     4441 ctgcgaacgt cttccacgtc gggcgcaggg ctggcgctgt cgatttcggt cggcgtgcta
     4501 accgcgggcc tgttttgggg cctctttcgc ccgggggccg accgtgggtt ctgggccggc
     4561 tttcaactgg cgggctgggg ttacctgctg gcagccgtca gcggctcgtc gcagtcccag
     4621 ctggggaatc atctaatcac gacacaactc gtgggggcgc tgtaccggca aatgccggtc
     4681 accagcaccc aaggcgtgat ggtcgagtgg cacggcacct ggtatgccgc cgaggtgctc
     4741 cgccgcagcg gggcgcggca ctacatccat tacacgggct acggagcgaa ttgggacgaa
     4801 tgggtcgggc ccgaccgcat ccgcggccag ttcggcccct ttctgcaaat ctgccacgcg
     4861 ctcttgagcc tgatcctggc cctggcgggc ggcgcagtcg gcggctgcct gggggttgtt
     4921 aaccggtccc ggcgctggtt ctggatcccg tggggcggct gtgcggccgc agccgtgggc
     4981 accagcctgc tggccatggc gaccgattcc gatctggccg ctggcgcagc gtggagcctg
     5041 ctgttggtcc tgctcgtgct ggctgccatt gccgcctggg gcggtccgga gcgccgcagc
     5101 agtttcgcgc tgggctttgc catcgtcggc tgcggctact tgctgctgca cttcggaccc
     5161 ggcctggaga ctacggtcgg cccgaatctg ctgagcacac gattgctcca attggttcac
     5221 gactggctgc acccggaagc ggcgcaaaca gtgagtcccg cgtggccgta caccggaacg
     5281 attggttccg gcagagtctc ctgggcgccg acctacgtcg gcatcggcta ttcgactgcc
     5341 gtgagcattt gcgtgctggc cggtcattgc ctgctggcca cgctgcttgg gctgctggga
     5401 ggcttcgcgg cgctgtggtt gtcgcggtgg cgaagacccg atcaaggccg gcaggacgcg
     5461 gctcatgcct gagagcgcca gcgccgggct cttgctcttt cgccggtcgg gcggggcgct
     5521 ggagctgttt ctggcgcatc ccggcggccc cttttggaag cacaaagacg caggcgcgtg
     5581 gaccattccc aaggggctga tcgaccgcgg cgaggagccg ctggcttcgg cgatacggga
     5641 gttcgaggaa gagacgggcc tgccgccgcg cggcccctac ctgccgctgg gcacgatccg
     5701 tcagaaagcc ggcaagacgg ttcacgcctg ggcgttcgaa ggggacgccg atccggcgaa
     5761 cgtgaagagc aacctggtgc agggctcgtt tcccgagatc gaccgctgcg cctggttcag
     5821 ccccgccgaa gcgcggcaga aactgaaccc cgctcaagct gccttcgtgg accgactgga
     5881 agcggcgctc cgcgagcaaa ggcaatcgga ttgataggcg tggatgcttc aagctggaac
     5941 gtgcggcacc ggaattgcaa atgccttccc aagacctcac ggagcattgc catggcgact
     6001 aagaaagcga cccggcggaa ggcttcccgg cgcgagctca tcgacacggg gcgcgacaag
     6061 cgatacgtcc gccgcgatgc gcaagggcaa ttcaaggaaa gcgatgacgt ggggcgttcc
     6121 ttgtctcaag atcgcaagcg caaggcgaaa tccgtcgcca aatcgggaca gggggaccag
     6181 ggagaccgca aacggggggc atgatcatga atcggccgtc cgaccatcct ccttgcccgc
     6241 tttgcggcca cagtggtcgc gagcaaatca cttctcccga acccgcgcga ttcccacctg
     6301 cgagcctggt gcggctcagg gtcttcaagt gccaaagctg cgggaccatc tattccgagc
     6361 ggcagaccac cgaagaagtc gccgaggcca gcgaagtagc ggcgtcgtaa gcccccagcg
     6421 ccttgctttg cacatgattg acaattactg aacagacagt acaatcgggt cggcgatttg
     6481 agattctggt cgtgaaggaa ccagcggcag gaatcgtgca tacggcccgg cttctggcgg
     6541 cttaatgtgc ataaaatgca tacgtgcatg cggcgtaaat cgccgtaact gcttgcggcc
     6601 actcggttta tgacagatga aatcgcgcgg aaaaatgccc ggaatatgca tgcatatgca
     6661 ctcagcctct gaagagccac gtccttttcc cgctacgggt ttggcttggc cactgtctcg
     6721 attcttgcgt ccggaacctt ggtctgcata agttgcataa ccatgcattg aggaaatcgg
     6781 cgcaagtcgt tgacagcata tgacctacgc caagaaacat gcaccggaaa aaccagcgaa
     6841 tatgcgggat aaggagccgg cggcgttgag taggatgcgg ccccatgaag tacaagatca
     6901 cccacacgac gatctacaac tacaccgagg cggtgccgat ctgccataac gaggtgcatc
     6961 tgacgccgcg cgagcataag cggcagtcgt gcctgagtca tcggctgcgg attcgcccgg
     7021 agccggcccg gatcgacacc cgaatcgatg ccttcggaaa ccacgccggc tggttttcga
     7081 tccaggaagg gcacgagcgg ctgtcggtaa cagcccacag ccaggtgcaa gtggccgagc
     7141 ccgacgtgcc cgcccccagc accacgccgc cttgggagga aatccgcgac cgctttgccc
     7201 tgggacagcc gccgtggtgg gtcgaagccc ggcagttcgt gtatgcctcg ccgcacgtgc
     7261 cgatcagcga gcaattgcgg gcgtatgctc agccgtcgtt tccggcgggg cagccgtggc
     7321 tggcggggtt gctcgacctg acgcggcgaa tcaaccagga atttcactac gatccggcag
     7381 cgacgacggt caacacgccg gtggcgaagg tgttgaagga ccgccgcggc gtctgccagg
     7441 attttgccca cctgcagatc gcctgcgtgc ggtcgctcgg cttgccggcc cgctacgtca
     7501 gcggttattt gcttaccacg cctcctcccg ggcagccgcg gctggtcggg gcggatgcct
     7561 cgcacgcctg ggtgtcggcg ttttgcccgg aggtcggttg gatcgatttc gacccgacga
     7621 acaatcagat tccatccacg cggcacgtca cgctggcctg ggggcgcgat tacagcgacg
     7681 tctgtccgat caaaggggtg ttggtcggcg gcgggcagca tcggatgcgg gtggccgtgg
     7741 atgttgtgcc gctggaggcc tgaatggaca ggaaatcggc ctcgcggaac cactagtcga
     7801 atcgtgggac ctggattatg ttgactccat cacctccggc ggccacccga gcccaccttt
     7861 gccgccggac ctggcaggga cgccttccca cccgcccttc ccttgggaga aatccatgcc
     7921 gactcgtttt gtcttcgctg cccttgcctt gctgggattg ctggctctga ccgattccgc
     7981 ttccgcgttc ggccgccgct gggagcgccg ccgggccgag ttgtacggct cgctcaatgc
     8041 atcgctttct gcccgcgtcg atgcgggtgt cgcccagcaa gcggcagcag ccgagcagcg
     8101 gctgagcgaa gccaacaagg cccagattgc ggcggaagcg gcgaagctga actcggaagt
     8161 agcagccgcc ctggcccagc tgaaacagca agcgaccgac gccgtcgccg ccgaagccag
     8221 gcgatttcag gatgaagcca aggcccagtt cgccgccctg cgcgaggctg cccagaacga
     8281 actgaaggcg gaattcgcca aagtccgcga cgaggccaag caggcggttg tcgccgagtt
     8341 caaccggacg cgggaggagc ttacgaagca agtcgcagct gaggcccaga aggtcaagga
     8401 cacgatgatg cccgagctga agaacgcggt ggcagccgag gttaagacgg cgctagcagc
     8461 cgcgaagccc gccgaagagc agcccgcgca aaagcagccc gagcagcccg ccgacaagcc
     8521 ggctgcctcc gccgccacga caacgacttc agaaaagaag gacgcgggcg agagcgagta
     8581 agccagccca cccaacatgc ccggcgatta tcccgagctc gatgtgatcg ccgtcggcgc
     8641 gcatcccgac gatgtggaga ttgcctgcgg cggcacgctg gccaagctcg tcaagcaggg
     8701 ctaccgcgtc ggcatcgtcg atctgaccga cggcgagccc acgccgctgt cgcccggccc
     8761 ggaaatccgc ctcgccgagg cccagcaagc ggccgccacg ctgggggcgc gggtccgcgt
     8821 cacgctcgag ctgcccaacc gccggctgtt cgacacgttc gaggcccgcg tgctcctggc
     8881 caaggagttc cgcaagtatc ggcccaagat cgtcatcggc ttcggcgaca aaacgccgat
     8941 ggcctcgccc gatcattggc aggcgatgca gatcaccgac gcggccgtct tctattcgcg
     9001 gctgacgaaa tgggacgagc actttgacgg cttgccggtc cacgcgatcc aggcccagct
     9061 ctatttccgg ctggccttcg aggcgatctc gggcggcaac tacccgaacc aattcacagt
     9121 ggacatcagc gacacgctgg agcagaagct ggcctcggtg cggtgctatg cgacacagtt
     9181 cccgccgggc aaggcccacg tcttcgaccg gatccgcggc gcggcgctga tcgccggcgc
     9241 ggcgtccagt tttgccgccg gcgaagtgtt catcagcacg cggccgctgg gcacgctcga
     9301 cctggtcaag accgtcctgc cgcggatgca agcgacctaa gccgctcgcg cgaccgccct
     9361 tgtttcacgc tcgccagcca tgctaccgtt ggtccatgcc ccatttgcct gatttcgacg
     9421 cgttccgccg cctggccgac gggcatcagc tcgtgccggt ctatcgccgg ctgctgagcg
     9481 acgcgctcac gcccgtctcg gcgtttcacc tgctggattc aggcggtcag gcgtgtctgt
     9541 tcgagagcgt gatcggcggc gagaaggtcg ggcgctacag ttttctggcc gtcgagccgt
     9601 tctgggagtt aattgcgcac ggaaagcgcg tgacggtccg cggcgaaggg ggccagcgga
     9661 atttcgagtc gtccgatccg ctcgacgagc tgcggcagcg tttgaacgcc tttcgggccg
     9721 tgcatctgcc cgagctgccg ccgttcgaag ggggcgcgat cggctatgcg ggctacgacg
     9781 tcgtgcgtta tgtcgagaat ttgcccaatg ccccgcccga cgatcgcggc ttgccggacc
     9841 tgtcgttcgc gttctacgac cggatggtcg tgttcgacaa cgtcagcaag gcgctgttcg
     9901 tgatcgcgat ggcgcggctc gacaagcagc cggacgcgcg ggccgcctac gacgacgctt
     9961 gccggcgcgt ggatgatctc gtcctgcgcc tgtcgcagcc aacgaacttg ccgccggccg
    10021 acatcgcgac gggcggcacg ccgcaagtcg cctggaagtc gaaccttaag caagaagagt
    10081 tcgaaaacgc ggtccgcaag tgcgttgagt acattcgcgc gggtgacatc ttccaggtcg
    10141 tcatcagcca gcggttggaa ctggagctcg gctgcgatcc gctggagctc tatcgcacgc
    10201 tgcgggtggt gaaccccagc ccattcatgt ttttcctgcg gacgcccgag gtcacgctcg
    10261 tcggcagctc tcccgagatc atgtgccgcg tcgtcgataa gcaggccaca gtccggccgc
    10321 tcgccggcac gcggccgcgc ggcgcgacgg acgaagagga tcgcaagctg gccgaagagc
    10381 tgctcgccga ccccaaggag cgggcagagc atgtgatgct cgtcgatctg ggccgcaacg
    10441 atatcggccg cgtggcaaag ttcggcagcg tcaagctttc cgacgtgatg gtcatcgagc
    10501 gctacagcca cgtcatgcac atcacgtcga acgtgaccgg caagctgcgc gacgagtgcg
    10561 acgcgttcga tgccctgaag gcctgtctgc ccgcgggcac ggtcagcggc gcgccaaaag
    10621 tccgggcgat gcagatcatc gacgaactgg agccgcaccg ccgcggcccg tacgccggcg
    10681 cagtgggata catcgactac gcgggcaaca tggatacctg cattgccctg cgtacgctcg
    10741 tcgtccaggg gcagcgggcc tacatccagg ccggtgccgg catcgtcgcc gacagcgttc
    10801 cagccagcga ataccaggaa acgctcaaca aggcccgcgg gctattaaag gcaattgaag
    10861 tgacccgaca acgagtcaag gcggagtagc cgcatcttgc ttcattccgc attccgaatt
    10921 ccgcactccg catttcttcc atgatcgtcg tcctcgctac catcgagctc caccccggca
    10981 agcggccgaa tttcctgacc gagttccggc agattgtgcc caaggtccgg gccgaagcgg
    11041 gctgcatcga gtacttcccg gcaatcgaca cggccagcgg cctgccggtg caggggccgg
    11101 cccgggaaga cgtcgtcgtg gtcgtcgaga agtgggaaag cctcgcggcg ctcgagaccc
    11161 acctcatcgc gccgcatatg atggagtacc gaccgaaggt gaaggatttc gtgaagaagg
    11221 tatcgcttca gattctgact ccagccgaat agaaatgacc gaagaaccga agaaccgaag
    11281 aaccaagtca gtagcttttc acttttggta attcggttct ttggttcttt gattttccca
    11341 tgcgtgatct cctccgccat ctcctcgcag ccctccgctc cggccggcca gtggcctggt
    11401 gccggctggt ggaaacgcgc ggcagcactc cgcaaaaggc gggtgcggcg atgctcgtct
    11461 ttggcgacgg ttcgcaggcc ggcacgctcg gcggcggctg cgtcgaagcc gaggtcaagc
    11521 ggcgggcgct cgtgctcgtc gccgaaaacc gcccggacgt ttgcgcgttt cagctcgaca
    11581 gcgattacgg ctgggacgac ggcctgatct gcggcggccg aatgcagatc gccgtcgagc
    11641 caatcgttgg taatggtgcg gcgccgtatt tcgagcggct atcggaaatc ttggaacgcg
    11701 ccggcggctg caccgaggcc atcgtctttg atcgccaggc gagcggcctg gcagcgccgg
    11761 cgagttttct cttcgacgcc agcggcgcat ttctggcggc aatgcgcggc gagagtccgc
    11821 cgccgcaggt tctcgatccg cttcgcccgc tcagcagccg gccgcgggcg tatgcagcgc
    11881 acggcgtggc ttatctgccc gtgctgcccc gctgcacgct ggtgatcgtc ggcggcggcc
    11941 acgtcggcaa agcagtggcc gatctcgccg ctgagttgga gttcgacgtt tggatcatcg
    12001 atgaccgggc ggagtatgtc gccgaaacgc gctttccgca ggtcgagcgg cgtatcagcg
    12061 gtccgatcga ccgcgtgctg cccgagctgg ccatcacggg cgacacctac tgcctgatcg
    12121 tgacccgcgg ccacagccac gaccaggagg cgctgtttca cctggccgat cggggcgcgc
    12181 ggtatgtcgg cctgatcggc agccggcgga agatcaagat gatcttcgaa aacctcgccg
    12241 ccgaaggcgt gtcgcgcgag gccctggcca aggttcacgc tccgctgggg atcgacatcg
    12301 ggtcgcagac cgtccccgag atcgccgtca gcatctgcgc cgaattggtc gcccaccgca
    12361 acttgggaaa ggtgccgggg cggccgcgcg gcgtcgagtt ttagcatggc ccgccacttc
    12421 gcgatcattc ccgcctgcgg ccagagcacg aggatgggcc ggccgaagct gctcttgccg
    12481 ctggaaggca agccgctggt ctggcacgcc ctggcagcgt ggcgcgccag cagcgtggcc
    12541 tcgatcacag ccgtcgtgcg ccgcgatgac acggaattgg cggcagcgct agcggaatct
    12601 ggagccgatg ttgtgcttcc gtcactaccg ccggcggaca tgaaggcctc cgttcaggca
    12661 gctctttcac atatcacaag tcgccatgcg ccctccgttg acgactcctt cctggtcgcg
    12721 ccggccgaca ttccgcggct ctcgccagcg atcgttgatg ctctcatttc gaggcacgac
    12781 gagcagcccg gttcgattct cgtgccgacg ctggctggcc gtcgtggaca tcctgtgctc
    12841 ttcccctggc tgctgtcgga acatgtattc agattgcaag aagacgaagg gctgaactcg
    12901 ctcgcgagcc ggtttccgcc ggtcgaagtt ccgtgcgacg ggctcgtgcc tgccggcagc
    12961 cggccgttcg ccgacatcga cactccggag caatatcaag cgttaggcgg cgaataccgg
    13021 gatgaataac ccagcgcccg aagtggcgag agcccaggga ttccttggta ctttcgcact
    13081 ttgtgcattt ggttcttccg ccgcgcctcg ctgctccgta cactaaacag accgattgtc
    13141 tattccgctc cagggctgct tggccctgag agcattagga tgcggaactg agggaacgag
    13201 gaacttcgcg atggcactgc gcagcacgcc gccagaaata cggcgcgatc ctgccgcggc
    13261 ccgcctgccg ctttcggttg tgctgctggt tgcgctcgcc ctgagcttcg tggccttgcc
    13321 gggctgcggc ggctgctggg gcggaggcgg cgcgaattcg gccgccgcca agaaaaagaa
    13381 ggaagaggaa gagaagaaaa agaaagagag caagaaaaag aagctcgaaa agcccaagga
    13441 cgacttcgag ccgatcgcca tccgcatgct gcccagcagc gacccgacgc agaacgtcaa
    13501 gaacccggcg atctttgcga agcccggcca ctggatcgcg ctaagcgaaa cgctcaaggc
    13561 caacaacttc gacttccccg gcgagctggc gacgttcgcc gaaacgccgg ccgacaatcg
    13621 gccgctcgtc gtcgaagaca cgacgcagcg attgtcgatc tggtcccccg ccatcctgcc
    13681 caaagggcag gccaagcgaa tcgagtcgct gatttatttg ccaaggcgct ctggtccgaa
    13741 cctcaaccgc acctacagcc tgcgcagcga gctgcacggc gtccgcggcg gccgcaccga
    13801 ggtctttgcc aacagccgca acgatgccct caaggattat caattcctga tcgtggtcct
    13861 gtcgagtaac cccgcggcct actcgcagtt cgcaaagctg aattcgattt acatgggcac
    13921 ggtggccacg cccgacacca acgagcttgg ccaggacacc ctgcggcact accacatcct
    13981 ggcgccgaac tccgaccgca ccgtcccgct gccgagccat ccgctggccc tgacgacgac
    14041 ttcgcacatc ttctgggacg acatcaaccc cgcaatcctc accgccctgc agcaggaggc
    14101 tttgctcgac tgggtgcatt ggggcgggca gctcgtgatc agcggccctg gctcgctcga
    14161 caagctccgc ggctcgttcc tggctcccta cctgcccgcg gattcctccc agacggtcaa
    14221 gctcgaacaa tccgcgttcg acgagctgaa ctctaactac tcgctgcaac gcgatcccgc
    14281 gcgggctggg caatcaccgc cgccgccgaa gggagaaccg ctgcggcgga tcaacgtcct
    14341 gcccgagcgt ccgatgctcg gcgtcgagct caagctgcac ccggccgctc agccgatccc
    14401 cggcaccggc ggcctggtgg tcgag
//
LOCUS       DASRJB010000032         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_858852, whole genome shotgun sequence.
ACCESSION   DASRJB010000032 DASRJB010000000
VERSION     DASRJB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858852"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFB80_00870"
     CDS             complement(<1..353)
                     /locus_tag="VFB80_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32329.1"
                     /translation="MRPYLAIIKDSFREAIASRVLWILLSLIGLQLLLMAPAGIRLNL
                     TTDFAWGDIGGGPELVTKLRRATIADAPSPGKRIWSLLGSETQRKLYQLEQAEEGENR
                     DFFQAMEVLRTGLTTL"
     gene            complement(448..1443)
                     /locus_tag="VFB80_00875"
     CDS             complement(448..1443)
                     /locus_tag="VFB80_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN32330.1"
                     /translation="MQPAIETQDLRKTYVEGLFRRKRQEALKGVSLRVERGEIFGLLG
                     GNGAGKTTFIKTLLGIIRKTGGQASLLGFPAGDRRGRKLVGYLPENLRVPRHLTGLTA
                     LEFYGHLSNVPTSIIKQRRGPLLEKVGLAERAKDPVRTYSKGMLQRLGLAQAMLHDPE
                     LFILDEPTDGLDPLARSQVRGYLTELKRQGKTIFLNSHILQEVELICDRVAILDRGVL
                     RRVANVAEITSGRVSITEGGNGQPHASQEALELHLDLTGPELAIRQALGNYPINSWQP
                     YSATDFRVVTRVPDQPAVDDLIDRLRASGLSIVGLSRRRVSLEEAYLEIVAEAAD"
     gene            complement(1539..2237)
                     /locus_tag="VFB80_00880"
     CDS             complement(1539..2237)
                     /locus_tag="VFB80_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32331.1"
                     /translation="MRILSYLGGSVIAAMTLGGVALAQQPAPQVPLGPTTVQLPTFSF
                     FTVQTTVSVPDRGGAYLGGINRGRDGRIERGFGPLANRAIGGDRSVASTSVHARIIDF
                     EEIDQMLLAAAARRAGTTVDPADAKAAALTKHVNGVAVVSAAGHSSSSPSGPLPGSIA
                     GIRAQNAAAAQERAAEVAELFAKAQQAEVEGKPAVAKIFYQMVVRRDAGALKQQAQAR
                     LSALAGKSSATAQR"
     gene            2339..>3746
                     /locus_tag="VFB80_00885"
     CDS             2339..>3746
                     /locus_tag="VFB80_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN32332.1"
                     /translation="MLRSLTQMAIALAAGIGLTPSPAAGQPAIKVPEGYTVELAAGPP
                     LVAHPIMAGLDDRGRLFVAENAGVNLQRPLLEEQLPNSILRLEDSDGDGKFDKRTVFA
                     DKLTFPQGALWHEGWLYSAVSGAIWRFQDQDDDGVAETREKIVSSFGYTGNAADVHGC
                     FLGPEGRIYWCEGRHGHDIVDKNGKSISKGKAARIFSCRPDGSDVRTHCGGGMDNPVE
                     IDWTAEGEMLGTVNIMYGKRGDCLVHWMHGGVYPRADQPEVLAEFRRTGELLDAVHDY
                     GHVAVSGCCRIRGAGIPPAQNSAATFLVTEFNTHKVLRTDLFRNGATFRAETSEYLQA
                     ASDDFHPTDVLEDADGSILVVDTGGWFRIGCPTSQVAKPDVLGAIWRVRRTGARIDDP
                     RGLRLYNSNEFDPGRLIAHLGDERPAVRERALADLARQGNAALVFLNRALVDKSAAVR
                     QNSIWTLARIGTPEATGAV"
BASE COUNT          632 a         1241 c         1222 g          651 t
ORIGIN      
        1 agcgtcgtga gccctgttcg tagcacttcc atcgcctgga agaaatcgcg gttctcgccc
       61 tcctcggcct gttcgagctg atagagcttg cgctgcgtct cgctgcccaa aagcgaccag
      121 attcgcttgc ccggcgacgg cgcatcggcg atcgtcgccc ggcggagctt ggtcacgagc
      181 tccggtccgc cgccgatatc gccccaggca aaatcggtcg tcaggttcag ccgtatgccg
      241 gccggcgcca tcaagagcag ttgaagcccg atcagcgaca gcaagatcca cagcactcgc
      301 gaggcgatcg cttcgcggaa cgagtctttg atgattgcca ggtagggacg catgggaaaa
      361 ttccaagaac cgaagatccg aagaaccaaa gtggattagt cggcgtgatt tggttcttcg
      421 gtcctttggt tcttcgattt ttgatcttca atcagctgct tccgccacga tctccaggta
      481 agcctcttcc aggctgacgc gacgccgcga gaggccgacg atgctcaagc cgctggcccg
      541 caggcggtcg atcaaatcat cgaccgccgg ctgatccggc acgcgggtga cgacgcggaa
      601 atcggtggcc gaatagggct gccagctgtt gattggataa ttgccgagcg cctggcggat
      661 cgccagttcg gggccggtca ggtcgagatg cagctccaga gcttcctgcg aagcatgcgg
      721 ctgcccattg ccgccttcgg tgatcgagac gcggccggag gtgatctcgg cgacgttggc
      781 cacccgccgc agtacgccgc ggtccaggat cgccacgcgg tcgcagatca gctcgacttc
      841 ctggaggatg tggctgttga ggaagatcgt cttgccctgc cgcttcagct cggtcaggta
      901 gccgcggact tgcgagcggg ccagcggatc gaggccgtcc gtcggttcgt cgaggatgaa
      961 cagctccgga tcgtgcagca tcgcctgggc caggcccagc cgctggagca tgcccttcga
     1021 ataggtccgc accgggtcct tggcccgctc ggccagcccg actttttcca gcagcgggcc
     1081 gcggcgctgc ttgatgatcg aggtcggcac attactcagg tggccataaa actccagcgc
     1141 cgtcagcccg gtcaagtgcc gcggcacgcg caggttctcg ggcagatagc cgaccagctt
     1201 ccgcccgcgg cggtcgcccg cgggaaagcc cagcaagctc gcctgcccgc cggtcttgcg
     1261 aatgatgccc agcagcgtct tgatgaacgt cgtcttgccc gctccgttgc cgccgagcag
     1321 accgaaaatt tcgccccgct cgacgcgcag gctcacgcct ttgagggctt cctgccgctt
     1381 gcggcgaaag agtccctcga cgtaagtctt gcgaagatcc tgagtttcga tggccggctg
     1441 cacgcggctg attccgacgc ggttatttgg cgagtctgct cgtcaaagtg taccacgccg
     1501 cccgctggct ggtttcagcg cagcccgccg tcagaagctc agcgctgggc ggttgcggag
     1561 gacttgccgg ccaatgccga cagccgcgct tgggcctgct gcttgagcgc acccgcgtcg
     1621 cgccgtacga ccatttgata gaagattttg gcgacggccg gcttgccttc gacttcggcc
     1681 tgctgggcct tggcgaagag ctcggcgact tccgcggcgc gctcctgagc ggcagccgcg
     1741 ttttgagccc tgatcccggc gatgctgccg ggcagcggcc cactgggcga ggacgagctg
     1801 tggcccgctg cgctgaccac agccacgccg tttacatgct tcgttagcgc cgccgctttc
     1861 gcgtccgccg gatcgactgt ggtccccgca cggcgggcag ccgcggccag cagcatctgg
     1921 tcgatttctt caaagtcgat gatccgggcg tgcacgctcg tgctggccac ggaccggtcg
     1981 ccgccgatgg cccggtttgc cagcgggcca aaaccccgct cgatccggcc gtcgcggccg
     2041 cggttaatgc cgccgagata agcgccgccg cgatcgggca ccgagaccgt cgtctgcacg
     2101 gtgaagaagc tgaacgtcgg cagctgaacc gtcgtcggtc ccagcggcac ctgcggcgcc
     2161 ggctgctgag cgagtgcgac gccgcccagc gtcatcgcgg cgatcaccga gccgccaaga
     2221 tatgaaagaa tcctcatcgg gccgcctccg cttgccccag ccttctgttt agcagattct
     2281 ggcgggaagg gaaagcgaac tggtggtcgc agtttcattg gagcgccgta tgacgcccat
     2341 gcttcgttca ctgacccaaa tggcgatcgc actcgccgcc ggtatcggtt tgacaccgtc
     2401 gcccgcagcc ggccagcccg cgatcaaggt ccccgagggc tacaccgtcg agctcgccgc
     2461 tggccctccg ctagtggcgc atccgatcat ggcggggctc gacgatcgcg ggcggctgtt
     2521 tgtggccgaa aacgcgggcg tgaacctgca gcggccgcta ctggaagagc agctgccgaa
     2581 ttcgatcttg cgcttggaag acagcgacgg cgacggcaag ttcgacaagc gcacggtctt
     2641 tgctgataag ctcacgtttc cgcaaggggc gctgtggcac gaaggctggc tctactcggc
     2701 cgtcagcggc gcgatctggc ggtttcaaga ccaggacgac gacggcgtcg ccgaaacgcg
     2761 cgagaagatc gtcagcagct tcggctacac cggcaacgcg gccgacgttc acggctgctt
     2821 cctcggtccc gaaggccgca tctactggtg tgagggccga cacgggcacg acatcgtcga
     2881 taagaacggc aagtcgatca gcaagggcaa ggcggcccgc atcttctcgt gccggccgga
     2941 cggcagcgac gtgcggaccc actgcggcgg cggcatggac aaccccgtcg aaatcgactg
     3001 gaccgccgag ggcgagatgc tcggcacggt caacatcatg tacggcaagc ggggcgactg
     3061 cctcgtccac tggatgcatg gcggcgtcta tccgcgggcc gatcagccgg aggtcctggc
     3121 cgaattccgc cgcaccggcg agctcctgga cgccgtccac gactacggcc acgtcgccgt
     3181 cagcggctgc tgccggattc gtggggcggg catcccgcct gcccagaatt ccgccgccac
     3241 gttcctcgta accgaattca acacgcacaa agtcctccgc accgatttgt tccgcaacgg
     3301 cgcgacgttc cgcgccgaaa cgagcgaata cttgcaggcc gccagcgacg atttccaccc
     3361 gaccgacgtg ctggaagacg ccgacggcag catcctggtc gtcgatacgg gtggctggtt
     3421 ccgcatcggc tgcccgacgt cgcaagtcgc caagccggac gtgctgggcg cgatctggcg
     3481 cgtgcggcgg accggcgcaa ggatcgacga tccgcgtggg ctgcggctgt acaactcgaa
     3541 cgagtttgat cccggtcggc tgatcgcgca cttgggcgac gagcggccgg cggtccgcga
     3601 gcgggccctg gccgatctgg cgcggcaagg aaacgccgcg ctcgtgtttc tgaatcgagc
     3661 gctcgtcgat aaaagcgccg ccgtccgcca gaattcgatc tggacgctgg cccgcattgg
     3721 cacgccagag gccaccggcg ccgtgc
//
LOCUS       DASRJB010000033         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_858992, whole genome shotgun sequence.
ACCESSION   DASRJB010000033 DASRJB010000000
VERSION     DASRJB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858992"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VFB80_00890"
     CDS             <1..1125
                     /locus_tag="VFB80_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN32333.1"
                     /translation="MPIVGKVWLVTTYDAVNELLRNHEQFVQSPQAAGNRWMSGLMRW
                     LPRNLRPLTTHMLIRDEPDHRRLRSLVEQAFQRQSVEALRPRLESLADEALDHLAQRA
                     AGSPRSVDLVEHFARPFPLSVICELLGLPPEDRPNFTRWGSAFTNARTVLGIVRGVFE
                     LSKLMRYVREEIQRQSQRPRGGLLAALIQAEESGDRLSEEELLAMVFLLLVAGHETTL
                     HQIAGSVLILLDHPDQLRELTADWSLADSAVQELLRYLSFAQLTKPRFARQDVEFCGR
                     QIRRGQILMACLASANSDPSRFENPDRLDLRRPANRHVAFGAGIHVCLGAKLARVETE
                     IALQRLFTRLPRLSLAIPRDRVQFTRRFGARGLVTLPVRW"
     gene            1174..1971
                     /locus_tag="VFB80_00895"
     CDS             1174..1971
                     /locus_tag="VFB80_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32334.1"
                     /translation="MSAATISDAEPATSQPPAGTASRMRLSIAHLLLWMAGTALVVAL
                     FPKGWLAGPDEFGSREIFLQRIAQRQTLERLSVVAVAPCYGAALASLLVAGVRLLWRR
                     PGFPSLPGHWPLVNLGLWVLIVGAIVGNVPDYSPTYNLHVVVQDAGVPLVILLMLLLT
                     VAVHGVVSVRQPLRWRIALGCQLAAAVLPVIGMVLMSGLNPAQLAITARLILLCWYIA
                     ALVSVLAGLWDFICRERFDLFHWVGVVSLLGILAHPPLTWLIADIAF"
     gene            complement(1977..3470)
                     /locus_tag="VFB80_00900"
     CDS             complement(1977..3470)
                     /locus_tag="VFB80_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN32335.1"
                     /translation="MMHRNAHWSVLTVAALLALALRLPAAERPNVLLILVDDLKPSFG
                     AYGERWVHSPNLDALAARGMRFDRAYCNQAVCAPSRNSLLTASRCTSLGVYTLGRNFR
                     RAVPDAVTLPQYFKQHGYHTAGIGKVFHIGHGNVGDEISWSEPFQPDKVVDYVLPEST
                     GGRLTREEALFANRGAAGLPRGAAWERADVQDDAYADGRIAAVGIQRLKAYRESKQPF
                     FLALGFTKPHLPFCAPAKYWDLYDRQKLPLPRVTAPPAGAPVYAGKTLGELNQYSPVP
                     DAPPLTEDLTRTLIHGYYAALSYMDAQVGRVLDELARTELAKNTIVVLWGDHGYHLGD
                     HGMWTKHTNYEQANRIPVVIVAPGVTAAGSSSAALIESVDIYPTLCELASLPPPAGPQ
                     PIDGKSLVPVLKDPRASIRDHAYHCFPRGQRLGRAIRTDRYRLVEWKQPGADAAAAEL
                     ELYDYQTDPDETKNLAAEQPEVVASLRAILERSGEAKPLEGAARGAK"
BASE COUNT          525 a         1229 c         1155 g          606 t
ORIGIN      
        1 atgccgatcg ttggcaaagt ctggctggtc acgacttacg acgcggtcaa cgagctcttg
       61 cggaaccacg agcagttcgt gcagagccca caggccgccg gcaatcgctg gatgagcggc
      121 ctgatgcggt ggctgccgcg gaacctgcgc ccgctcacga cgcacatgct catccgcgac
      181 gagcccgacc atcgccggct gcgaagcctg gtggagcagg catttcagcg gcaaagcgtc
      241 gaggccctcc gcccccggct ggaatcgctc gccgacgaag ccttggatca tctggcccag
      301 cgggcggccg gctcgccgcg cagtgtcgat ttggtggagc acttcgcccg cccgttcccg
      361 ctctcggtaa tttgcgagct gctcggcctg ccgccggagg accggccgaa tttcacgcgc
      421 tggggatcgg cgtttacgaa cgcccgcacg gttttaggca ttgttcgcgg ggtattcgag
      481 ctcagcaagc tgatgcgcta tgtccgcgag gaaatccagc gccagtcgca gcggccccgc
      541 ggcgggctgc tcgcggcgct catccaggcg gaagaatccg gcgaccggct gagcgaggaa
      601 gagctgctgg cgatggtctt cctgctgctc gtcgcgggcc acgagacgac gctgcaccag
      661 atcgccggca gcgtgctgat cctgctcgat catccggacc agctccgcga gctgaccgcc
      721 gattggagcc tggccgactc ggccgtgcag gagctgctgc gttacctgtc gtttgctcag
      781 ctgaccaagc cccgcttcgc ccggcaggac gtggaattct gcggccgcca aatccgccgc
      841 gggcagattc tcatggcctg cctcgcgtcg gcgaacagcg atcccagccg gtttgagaat
      901 cccgaccgcc tggacctccg ccgcccggcc aaccgccacg tcgcctttgg ggccggcatt
      961 catgtctgcc tgggggcgaa gctggctcgc gtcgaaacag aaatcgccct gcagcggctc
     1021 ttcactcgct tgccgcggct gtcgctggcg attccgcgcg accgggttca attcacgcgc
     1081 cgcttcgggg cgcgcggcct ggtgacgctg cccgtgcggt ggtgacggac catcgcgaac
     1141 atcgcggagc gggccgtcgt ataaactggc gccatgagcg ccgcgacgat ctccgacgcc
     1201 gaacccgcga ccagccagcc gccggccggc acggcgagca ggatgcggct ctcgatcgcc
     1261 cacctgctgc tctggatggc cgggacggcg ctggtcgtgg cgctctttcc caaaggctgg
     1321 ctcgccggtc cggacgaatt cggcagccgc gagatcttcc tgcagcggat cgcccagcgg
     1381 cagacgctgg agcgattgag cgtcgtcgcc gtcgcccctt gctacggcgc agccctggcg
     1441 agcctgctcg tcgccggcgt gcggctgctc tggcggcggc ccggctttcc gtcgctaccg
     1501 ggccactggc cgctcgtgaa tctcggcctg tgggtgctaa tcgtcggcgc gatcgtcggc
     1561 aacgtgccgg actacagccc cacctacaac ctgcacgtcg tagtccagga cgcgggcgta
     1621 ccgctcgtga ttctgctgat gctgctgctc accgtagcgg ttcatggcgt cgtgtccgtc
     1681 cgccaaccac tgcgatggcg gatcgccctg ggctgccagc tagccgcggc tgtgctgccg
     1741 gtcatcggca tggtgctgat gagcgggctg aacccggcgc agctggcgat caccgcaagg
     1801 ctgatcctgc tctgctggta cattgcggcc ctggtttcgg tgctggcggg cctgtgggac
     1861 ttcatctgcc gcgagcgctt tgacttgttc cactgggtcg gcgtcgtcag cctgctcggc
     1921 atcctcgccc acccgccgct cacctggctg atcgccgaca tcgcgttttg aagcgcctat
     1981 ttcgctccgc gggccgcgcc ctccagcggc ttggcttcgc cggagcgctc gaggatcgcc
     2041 cgcaggctcg ccacgacctc cggctgctcg gccgccagat tcttcgtctc gtccgggtcg
     2101 gtttgatagt cgtacagctc cagttcggcc gcggcggcgt ccgccccggg ctgcttccat
     2161 tcgaccagcc ggtagcgatc ggtgcggatc gcccggccca gccgctggcc gcgcggaaag
     2221 caatgatagg cgtggtcgcg aatgctcgcc cgcgggtcct tgagcacggg cacgaggctc
     2281 ttcccgtcga tcggctgcgg gcccgccggc ggcggcagag acgccagctc acagagcgtg
     2341 ggatagatat cgaccgactc gatcagggcc gccgagctgc tgcccgcggc cgtcacgccc
     2401 ggcgcgacga tgacgaccgg aatccgattg gcctgctcgt agttcgtgtg cttggtccac
     2461 atcccgtggt cgcccaagtg gtagccgtga tcgccccaca ggacgacgat cgtattcttc
     2521 gccagctccg tccgcgccag ctcgtccagc acgcggccga cttgcgcgtc catgtagctc
     2581 agcgccgcgt agtagccgtg aatcagcgtc cgcgtcaggt cctcggtaag cggcggcgcg
     2641 tcgggcacgg gcgaatactg gttcagctcg cccagcgtct tgccggcata caccggagcg
     2701 ccggccggcg gcgcggtcac ccgcggcagc ggcagcttct ggcgatcgta gaggtcccag
     2761 tacttcgccg gcgcacaaaa cggcaagtgc ggcttggtga agcccagcgc caggaagaac
     2821 ggctgcttgg attcgcgata cgccttcagc cgctggattc ccacggcagc gatgcggccg
     2881 tcggcatagg cgtcgtcctg gacgtcggcc cgttcccagg cggctcctcg cggcagaccg
     2941 gctgcgccgc ggttagcgaa cagggcctcc tcgcgggtca gccggccgcc ggtgctctcg
     3001 ggcagcacgt aatcgacgac cttgtccggc tgaaacggct cgctccagga aatctcatcg
     3061 cccacgttgc cgtggccgat gtgaaagacc ttgccgatgc ccgcggtgtg atagccgtgc
     3121 tgcttgaaat actgcggcag cgtgaccgca tcgggcaccg cccggcgaaa gttgcggccc
     3181 agcgtataga cgccgagcga cgtgcagcgg gacgccgtca gcaggctgtt ccgcgacggc
     3241 gcgcagaccg cctgattgca atacgcccgg tcgaaccgca tcccccgcgc tgccagcgca
     3301 tccaggttcg gcgaatgcac ccatcgctcg ccgtacgccc caaacgatgg cttcaagtca
     3361 tccaccagga tcaacagcac attcggccgc tcggcggccg gcaatctgag tgctaatgcc
     3421 agcagagccg cgacagtcaa aaccgaccag tgggcgtttc gatgcatcat gccggcatct
     3481 tcatccactg ggatgcacga atggaagccc ccgca
//
LOCUS       DASRJB010000034         9659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_859278, whole genome shotgun sequence.
ACCESSION   DASRJB010000034 DASRJB010000000
VERSION     DASRJB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859278"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFB80_00905"
     CDS             <1..486
                     /locus_tag="VFB80_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32336.1"
                     /translation="IEKIVINVREQTRPERIKTGFEGFDKSKFTVFTAVLDVEGQGEI
                     WLYNRPDGEMLKLHPGDKFEVGSIKGTVAEIGEYEFLFDNGGKRLKLAHGENLFNAKV
                     VGDTPGALPPVTVETSTPAPAPPSPAEQPAPPSAEKPGEGDKAAEAAPAEAPASDAKA
                     G"
     gene            823..4587
                     /locus_tag="VFB80_00910"
     CDS             823..4587
                     /locus_tag="VFB80_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN32337.1"
                     /translation="MIALRRNEFHRWAASGAACLWLAAIAWGQAPGGPAPLPRPAGPF
                     PQPPAQASFAQPGVIPASAVGQIPLQATLPVQVPFPMQAAPPSGVMLTSAAPPAPPTP
                     RLVTQSWQLRNLNWREFENGLNRTWGTRLESSQDPAGDMGTFRFPERPAGSASFMVDH
                     RNNHVTITSPIQTAASWLRLAQVMDTRPQAGDERTAVASLDKADPRTVQKALALFQRG
                     ARPARSANHPGRRKEHIGEFVSMLFQQGAGGAQPGFQPVPGFQPPLPGFQPPGADVQG
                     DFLQVAPTPADTGVAGALQRLAGNVQIEILDDIVIVRGRREDVEKVMQLIEQIEQQSI
                     EFRPEVEIYYLKNIDSQQLNLVIEQVYATVFARQGLVTRVPLVDPNAILLIGRKENIP
                     AVVELIDKLDKPLPPTSQFKVFLLKYMSCIDAERTVKQFFSADPMASNLPVPRNGTGI
                     RPYVIAEFRANALIVQAGPRDMAEVERLIASLDIDTTPAPGEVRVFKLKNSLAETLAP
                     VLLEAIAGIGGAQQQQQQQPQAQQGGTSPARATERATSLQFLIPGGNELIRSGVLRDM
                     RITADIGSNSLIVVGPPSGMPLMEAIINQLDGLPPTEAQIKVFSIVNGDATALSTMLQ
                     TLFGQAQQGAQGQQGLNQSPTGAGESALVPLRFSVDQRTNTIIATGNPADLEVVRNII
                     LFLDVSGQTERINTVIRLHNAPALDVATAITNLLAQQRTLNQAAPELVTPYQQIEREI
                     LVEPELVTNSLIVSATPRYFERLQAIIKELDKRPPMVTIQVLIAEVTLNDDEQFGVEW
                     GLQDSLLFDRGIPPAAGAALQPGFAFNSIILPNQNSPQSLATRENVAGQALSNLALGR
                     IDPTLGFGGLVLTASNESVNVLLRALERSQRLQVISRPQVQTLDNQLAYVNVGALVPR
                     ITGIAQNQVGITNPQLEDISVGIILEVTPRTSPDGTIVMQVNATKSSVGPDATGIPVF
                     TDANGNVVRSPQIPLTTAQTTVSAKSGQTVILGGLITKSLTESTRRVPYLADIPVLGR
                     LFRFDTVSNQRTELLIILTPYMMQSAEQNEWLNQRESERMSWCLADIVNIHGPVAMSG
                     NPAYNMHSSEFIFPDLTPAGPQPTPATQPPLDPLMPGGTGPQPYYAPPAANPPSATPP
                     PPAPMLTPPMLPPPGQQPSSVIVPPPAGLTPLPPGEQQAFGDAAPAEPGFVPPPPLKG
                     LPPSPDQRPLTPQTLQPAVPPAGTQAALRQRQAAQQQFVVPANYQQPVVR"
     gene            4805..5791
                     /locus_tag="VFB80_00915"
     CDS             4805..5791
                     /locus_tag="VFB80_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32338.1"
                     /translation="MRPNSSLVALAAACLLAGCQSMPAPHWHAPEQPKVTESKFSRPV
                     RLAVIWSPALLNTPGGKPTRGFGGRIYFYDTDNNAVPVEGQLIVYGYNNKPGAGTKMP
                     DRKFAFTPEQFTAHFSPTDLGASYSVWLPWDEVGNEQVEVSLVPIFTAASGQLIVGQS
                     HGLLLPGPTTENAATQFDHRKLGMNEMVRRPYPGQPAMPGVQQASYLAPPVASNLPPS
                     ARERELTIKLSGSLAERIAAAPLPPGPQVEAAARERATAAILAALKTAQPAPSTAAQP
                     NAAPTAAPPAAPPRPWSPTNQPSARFLRPTLPDPSSPGLPPVAGRLPTRPSP"
     gene            6706..8463
                     /locus_tag="VFB80_00920"
     CDS             6706..8463
                     /locus_tag="VFB80_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32339.1"
                     /translation="MKRLISRSMLPMGVLAGWLLALAPAFAQRPVAPKLLPVETIAIL
                     RIADTPQLIERFRQTSLGKIGRDESVKPLVSSLYGSLKEAWGQIEEQVGLPLDQVLGI
                     PQGEVCVALVAPPEQRPGVVFFLDVKDRMAQVKKLLASGETFLQDNGGTRITEQIENQ
                     EVAIYSGRGNTQFYLIERDGSIVIATTKELARSVLIAWNGGAEKSLADNDKYNAVMSR
                     CAGSVTDPPQVTFFVDPIEGVKAFARGSFAATGLALLPVLGLDGLKGVGGSMTFATGE
                     FDEVQHFHLLLDNPRAGVIEAIAMKSGDSTPEVWVPSDVVSYTTLHWDFQRTFNVSAK
                     LYNGLMEENALQNEVKRRISDRLGVDFEKEILPLMAGRVTFSQWVEKPVRINSITTIF
                     GIKVKDPKAVQPVLDQVVARFGQNLEKQRFGSVSYWSIKVPQRQQDRPNLRQPSPCFG
                     VIGDCLVFSDSMGAFRECVLTSSDLSRGLANSLDYKLIASKIKRQPGGDAPGMVQFSR
                     PEEGMRFWYDLATADTTKQALASQAEDNRFFGSLDKALKDTPLPPFAVIAQYLAPGGG
                     MMVNDETGLHYSSFTLKGK"
     gene            8494..9357
                     /locus_tag="VFB80_00925"
     CDS             8494..9357
                     /locus_tag="VFB80_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32340.1"
                     /translation="MLTVFALASGRSGTHFLYELIRRNARSCAARHEPYGFNPSMFGR
                     PIYDRAVGERDYTRRLVARKARIIARCGQPAYVETSHAFLKSWSDLAAEYFPNLKLVH
                     LVREPLKVAKSEAYRETWLHRLHLPLRNYRGGDGRKYFRWALTGLEPIFQHFERAQLT
                     RLQWYLLQWIEIENRAMQFLAASGKQANCFTLHSPHELSDAGRVRELFEFLGLSLKHK
                     TLDLRGRQNRNPRPTVITDDDRRELADVVGRLPPEHLAIFQREPYVRFPWIESLRLGG
                     TGVSQVSGRVF"
BASE COUNT         1719 a         3276 c         3090 g         1574 t
ORIGIN      
        1 atcgagaaga tcgtcatcaa cgtccgcgag cagacccgtc cggagcgcat caagaccgga
       61 tttgagggct tcgataaatc caagttcacc gtcttcaccg ccgtgctcga cgttgaaggc
      121 cagggcgaaa tctggctcta caatcgcccc gatggcgaga tgctgaagct gcatcccggc
      181 gacaagtttg aggtcggctc gatcaaaggc accgtggccg agatcggcga gtacgagttc
      241 ctgttcgaca atggcggcaa gcggctgaag cttgctcacg gcgagaacct gttcaacgcc
      301 aaggtcgtcg gcgacacgcc gggggccttg ccgccggtga cggtcgaaac ctctacgccc
      361 gcccccgctc cgcccagtcc cgcggagcag cccgcgccgc cgtcagccga gaagcctggc
      421 gaaggggaca aagcagcgga agctgcgccg gctgaagcgc ccgcgtccga cgccaaagcg
      481 ggataaatca ggcagggatg ctggcactcg ctggccggca tggactccgg ggttaagcag
      541 cgcggcgatt tgacgcagca ctcccaatct gcagccgatc catcgccgca accttttttg
      601 ccgtctgatc ttgcgccgct tcgcgggccg ggctgttttc tctgaaacag ttcacgccga
      661 aggcaaactt tgccgattat tccagttgta ccggatattc cggcggccat gcgcgcgctt
      721 tgcgttcgct atcgccacgg agaattcgtt tggggctgtg agaccgagag gggcattgcg
      781 ccggccgcgg cagccgtggt cggaccaagg aagattcagc ccatgattgc actacgacga
      841 aacgaatttc accgctgggc ggcaagcggc gcggcgtgcc tgtggctggc agcgatcgct
      901 tgggggcaag cgcctggggg gccagcgccg ctgccgcgcc cagcggggcc atttccacaa
      961 ccgcccgcgc aggcctcgtt tgctcagccg ggcgtgatcc ccgcgtccgc cgtcggtcag
     1021 ataccgctgc aggctacgct gccggtgcag gttccctttc caatgcaggc cgcgccgcct
     1081 tcgggcgtaa tgctgacttc ggccgctcca ccggcgccgc cgacgccgcg actggtgacg
     1141 cagtcgtggc agctccgcaa cttgaattgg cgcgagttcg agaacggcct gaaccgcact
     1201 tggggcacgc ggctggagtc ctcgcaggac ccggccggcg acatgggcac gttccgcttt
     1261 ccggagcggc ccgccggatc ggccagcttc atggtcgatc accgcaacaa ccacgtaacg
     1321 atcacctcgc ccattcagac ggccgcctcg tggctgcggc tggcgcaggt catggacacg
     1381 cggccgcagg caggcgacga gcggacggcg gtggcctcgc tcgacaaagc ggacccgcgg
     1441 accgtgcaga aggcgctggc gctctttcag cgcggcgcgc ggccggcccg gtcggcgaat
     1501 catccgggac ggcgcaagga acacatcggc gaattcgtgt cgatgctctt ccagcagggc
     1561 gccggcgggg cgcagcccgg attccagccg gtgccggggt ttcagccgcc gctgccggga
     1621 ttccagccgc cgggggccga cgtgcaggga gacttcctgc aagtcgcgcc gacgcccgcc
     1681 gacaccggcg tcgccggcgc gctgcagcgg ctggcgggca acgtgcagat cgaaatcctc
     1741 gacgacatcg tgatcgtccg cggccgccgg gaagacgtcg agaaggtgat gcagctcatc
     1801 gagcagatcg agcagcagag catcgagttc cggcccgagg tggaaatcta ttacctcaag
     1861 aatatcgatt cgcagcagct caacctggtg atcgaacagg tctatgcgac cgtcttcgcc
     1921 cggcagggcc tggtgacgcg ggtcccgctg gtcgatccca acgcgatcct gctgatcggc
     1981 cgcaaggaga acatcccggc cgtcgtcgag ctgatcgaca agctcgacaa gccgctgccg
     2041 ccgacctcgc agttcaaggt cttcctgctg aagtacatgt cgtgcatcga cgccgagcgg
     2101 acggtcaagc agttcttctc ggccgacccg atggcctcca acctgccggt gccgagaaac
     2161 ggcacaggca tccggccgta cgtgatcgcc gagttccggg ccaatgccct gatcgtgcag
     2221 gccggccccc gcgacatggc cgaggtggag cggctgatcg cctcgctcga catcgacacc
     2281 actcccgcgc ccggcgaggt gcgggtcttc aagctcaaga attcgctggc ggaaacgctg
     2341 gcgccagtgc tcctggaagc gatcgccggc atcggcggcg cgcagcaaca gcagcaacag
     2401 cagccgcagg cccagcaggg cggcacttcg ccggcccggg ccacggagcg ggccaccagc
     2461 ctgcagttcc tgatcccggg cgggaacgaa ttgatccgct cgggcgtgct ccgcgacatg
     2521 cgaatcacgg ccgatatcgg tagcaactcg ctgatcgtcg tcggtccgcc cagcggcatg
     2581 ccgctgatgg aggcgatcat caaccagctc gacggtctgc cgccgaccga ggcccagatc
     2641 aaggtcttca gcatcgtcaa cggcgacgcg accgcgctct cgacgatgct ccagacgctg
     2701 ttcggccagg cccagcaggg cgcccagggc cagcagggtc tgaaccagtc gcccacgggc
     2761 gcgggtgaaa gcgcgctcgt gccgctgcgg ttcagcgtcg atcagcggac caacacgatc
     2821 atcgccacgg gcaacccggc cgacctggag gtcgtccgca acatcatcct gttcctggac
     2881 gtctccggcc agaccgaacg catcaacacg gtcatccgcc tgcacaacgc gccggccctc
     2941 gacgtggcca cggcgatcac gaacctgctc gcgcagcagc ggacgttgaa ccaggccgcg
     3001 ccggaactgg tcacacccta tcagcagatc gaacgcgaaa tcctggtcga gccggaattg
     3061 gtcaccaaca gcctgatcgt cagcgccacg ccgcgctatt tcgagcggct gcaggcgatc
     3121 ataaaggagt tggacaagcg cccgccgatg gtcacgatcc aggtgctcat cgccgaagtg
     3181 acgctgaacg acgacgagca gttcggcgtc gagtggggtc tgcaggactc gctgctgttc
     3241 gaccgcggca ttccgccggc cgcgggcgct gcgttgcagc cgggctttgc cttcaattcg
     3301 atcatcttgc cgaaccagaa ctcgccgcag tcgctggcca cccgcgaaaa tgtcgcgggc
     3361 caggccctct cgaacctggc cctgggccgc atcgatccga cgctgggctt cggcggcctg
     3421 gtgctcaccg ccagcaacga gtcggtcaac gtcttgctgc gggctctgga gcggtcccag
     3481 cggctgcagg tcatcagccg gccgcaggtg cagacgctcg acaaccagct cgcgtatgtc
     3541 aacgtgggcg cgctcgtgcc gcgaatcacg ggcattgcgc agaaccaggt cggcatcacc
     3601 aatccgcagc tggaagacat ttccgtcggc atcattctgg aagttacgcc gcggaccagc
     3661 ccggacggca ccatcgtgat gcaggtcaac gcgaccaagt cgtcggtcgg ccccgatgcg
     3721 acgggcattc cggtctttac cgacgccaac ggcaacgtcg tccgctcgcc gcagattccg
     3781 ctcaccaccg cccagacgac cgtctcggcc aagagcggcc agaccgtcat cctgggcggc
     3841 ctgatcacca agagtctcac cgagagcacc cgccgggttc cgtatctcgc cgacatcccg
     3901 gtgctcgggc ggctgttccg ctttgacacc gtcagcaacc agcgaacgga gctgctgatc
     3961 atcctcacgc cctatatgat gcagtcggcc gagcagaatg agtggctcaa ccagcgggaa
     4021 agcgagcgga tgagctggtg cctggccgac atcgtgaaca tacacggtcc ggtcgcgatg
     4081 tcgggcaacc cggcctacaa catgcactcc tcggagttca tcttcccgga tttgacgccg
     4141 gccgggccgc agccgacgcc ggcgacccag ccgccgctcg acccgctgat gccgggcgga
     4201 accggcccgc agccgtacta cgctccgccg gccgcgaacc cgccgtcagc cacgccgccg
     4261 ccgccagcgc cgatgctgac gccgccgatg ctgccaccgc ccgggcagca gccttcgagc
     4321 gtgatcgtgc cgccgcccgc tgggctgacg ccgctgccgc caggcgagca gcaggccttc
     4381 ggcgacgccg cgccggcaga gcccggcttc gtaccgccgc cgcccttgaa ggggctgccc
     4441 ccgtcgcccg accagcgccc gctgacgcca cagaccctgc agccggcggt tccgcctgcg
     4501 ggcactcagg ccgcgctccg ccagcggcaa gccgcgcagc agcagttcgt cgtgccggca
     4561 aattatcagc aaccggtggt gaggtgatac aacgaggccc ggagggccga aagtcgggga
     4621 ttcgtgcgaa aaaaaaggcc atcaggaggc ccggagggcc ggcaccggcg cgacaatgtc
     4681 gcttccgcca tggtgtcggc cctccgggcc ttccaatcaa taccgaattt ggcgtgtccc
     4741 ccgacctcac ggtcggggct actgcctgcc ggccctccgg gccttgagac caaggaacga
     4801 cgccatgcga ccaaactcgt ctctcgtggc actcgcggcg gcctgcctgc tcgccggctg
     4861 ccagtcgatg ccagcgccgc actggcacgc gcccgagcag ccgaaggtga cggagagcaa
     4921 gttctcgcgg ccggtgcggc tggcggtgat ctggtcgccg gcgctgctca atacgcccgg
     4981 cggcaagccg acccgcgggt tcggcgggcg gatctatttc tacgacaccg ataacaacgc
     5041 cgtgcccgtg gaagggcagc tcatcgtgta cggctacaac aacaagcccg gcgccggcac
     5101 caaaatgccc gaccggaaat ttgctttcac ccccgagcaa ttcacggccc acttcagtcc
     5161 gacggatctc ggcgcgagct acagcgtctg gctgccctgg gacgaagtcg gcaacgagca
     5221 ggtcgaggtc agcctcgtcc caatcttcac cgccgccagc gggcaactga ttgtcgggca
     5281 atcgcacggc ctgctgctgc ctggaccgac gacggagaac gcggcgacgc agtttgacca
     5341 tcgcaagctc gggatgaacg agatggtccg gcgtccttac ccgggacagc ccgcgatgcc
     5401 gggcgtgcag caagcgagct atctcgcacc accggtggcc agcaatttgc cgccgtcggc
     5461 tcgagagagg gaactgacaa tcaagctgtc cggctcgctg gcggaacgga tcgccgcagc
     5521 accgcttccg ccgggaccgc aagtcgaagc cgcagctcgc gaacgggcga ccgcggccat
     5581 cctggcggca ttgaaaaccg cgcagcctgc cccatcaact gccgcgcagc ccaacgccgc
     5641 gcctacggcc gcgccgccgg cagctcctcc gcggccctgg tcgccgacga atcaaccttc
     5701 agctcgtttc ttacggccaa cacttccgga tccatcttcg ccagggcttc cgccagtcgc
     5761 cggtcgtctt ccgacgcgac cgtccccttg agaacagccg tccgcccctc gagcattacc
     5821 tccggcgcac ccacaaagcg gactcccggc agcctcgtaa gccgcgtttc gaagttgcgg
     5881 acgtgtgtca gcgagacggg ctgcggctga aagcccatcc gcagcggcgt gcgaatcttc
     5941 gggcgggtgg cgcgctgatt ctgctggttg agctgctgca ggccctgcga gaacaggtta
     6001 cccaattgat tgaggccgct ggtattgctg cgcccagccg cacctccctg agcacctccc
     6061 tgaagcgcca tgaggctgcg ggcattcgtc acgcccgcgt cggaagagtc ggccccgacg
     6121 aacgcgcccc gcgtttgatt caaggtcact gccgaggctg cattttgcgc tccaatcgcc
     6181 acattaggag cgccggtaga agcggcagca gaaacgcccc cctgggtcgt tcccgtggtg
     6241 ccgccagcgc tggttcccgt gctggtgcgg gcgcccgttg aggccgtcgg cgtgcgcgat
     6301 gcggcactgc ccccgacgct ggtctgaccg aacatgccat ttcgggtcga ttgcccgaaa
     6361 gctgcgtccg ccagcccgaa cgcgaccacc acgcccgccg caatcagcca tgcagtacgc
     6421 atcaccgcac ctcttcagca aaggccgaca ataccctatt caccgtttcg tccgcggctg
     6481 agccggccgc gtacaatacc agcctgctgc accgaacatg ccggtcagcc aagctttgtc
     6541 ggcccaatat ccattttgag gccgcagccg gatgctggca agcgcgcagc agagtataaa
     6601 ccccaatcgg gcctgcaagt tgcggctgct tgaaacccgc taccgccgtt tggggtatta
     6661 accccgaatt ctgccaaaac tgcctttact ccgggggctt ttgccgtgaa acggttgatt
     6721 tcgcgttcga tgctgccaat gggcgtgctg gctggctggc tgctggcgtt ggcgccggcc
     6781 ttcgcccagc ggccggtggc cccgaagctg ctgccggtgg agacgatcgc catcctgcgg
     6841 atcgcggata cgccgcagct gatcgagcgg ttccgccaga cgtcgctggg caagattggc
     6901 cgcgacgaga gcgtcaagcc gctggtttcc agcctctacg gctcgctgaa agaagcctgg
     6961 gggcagatag aggagcaggt cggcctgccg ctggaccagg tgctgggcat cccgcaggga
     7021 gaagtctgcg tcgcgctggt cgcgcccccc gagcagcggc cgggcgtcgt ctttttcctg
     7081 gacgtcaagg accgcatggc ccaggtgaaa aagctgttgg ccagcggcga gacctttctg
     7141 caagacaacg gcggcacacg gatcaccgag cagatcgaga accaggaagt cgcgatctat
     7201 tcaggccgcg gcaacacgca gttctacctg atcgagcgcg acggctcgat cgtgatcgcc
     7261 accacgaaag agctggcccg ttcggtgctc atcgcctgga acggcggcgc cgagaaatcg
     7321 ctggccgaca acgacaaata caacgcggtg atgagccgct gcgccggctc ggtcaccgat
     7381 ccgccgcagg tcacgttctt cgtcgatccg atcgagggcg tgaaagcctt tgcccgcggc
     7441 tcgttcgcgg ccaccggcct ggcgctgttg ccggtgctgg gactcgacgg cctcaaagga
     7501 gtcggcggca gcatgacttt cgccaccggc gaattcgacg aagtgcagca ttttcacttg
     7561 ctgctggata atcctcgggc cggcgtgatc gaggcgatcg caatgaagtc gggcgactcg
     7621 acgcccgaag tttgggtgcc cagcgacgtc gtcagctaca ccacgctgca ctgggatttt
     7681 cagcggacgt tcaacgtctc ggccaagctc tacaacggac tgatggagga aaacgccctg
     7741 cagaacgaag tgaagcggcg gatcagcgac cgcctgggcg tcgatttcga gaaggaaatt
     7801 ctgcccctga tggccggccg cgtgaccttc agccagtggg tggaaaagcc cgtgcggatc
     7861 aacagtatca cgacgatctt cggcatcaag gtgaaggacc ccaaggcggt ccagccggtg
     7921 ctcgatcagg tcgtcgcccg cttcggccag aacctggaga agcagcggtt cggcagcgtc
     7981 agctactggt cgatcaaggt gccccagagg cagcaggatc ggccgaacct gcggcagccg
     8041 tcgccctgct tcggcgtgat cggcgactgc ctggtgttca gcgatagcat gggggcgttc
     8101 cgcgaatgcg tgctgacctc cagcgatctc agccgcggcc tggccaactc gctcgattac
     8161 aagctgatcg ccagcaagat caagcggcag ccgggcggcg atgcgccggg catggtgcag
     8221 ttcagccggc cggaggaagg gatgcgattc tggtacgatc tggccaccgc ggatacgacg
     8281 aagcaggcgc tagcgagcca ggccgaggac aatcggttct tcggctcgct cgacaaggcc
     8341 ctcaaggaca ctccgctgcc accgtttgcg gtaattgccc agtatctcgc tcccggcggc
     8401 gggatgatgg tcaacgacga gacgggcctg cactactcgt cgtttaccct gaaggggaag
     8461 tagcggcgga gatcgccatc aggccgccat tcgatgctca ccgtcttcgc tctcgccagc
     8521 ggccggtcgg ggacgcactt tttgtatgag ctgattcgcc gcaatgcgcg atcgtgcgcg
     8581 gcgcggcatg agccgtatgg gttcaatccg tcgatgttcg gccggccgat ttacgatcgc
     8641 gcggtcggcg agcgggatta cacccggagg ctggtggccc gcaaggcgcg gataatcgcc
     8701 cggtgcggcc aaccggcgta tgtcgagacg agccatgcct tcctcaagtc gtggtcggat
     8761 ctggccgcgg agtatttccc caacctgaag ctcgtacacc tggtccgcga accgctcaag
     8821 gtggctaaaa gcgaggcgta tcgcgagacc tggctgcacc gcctgcatct gccgctgcgg
     8881 aactaccgcg gcggcgacgg gcggaagtac tttcgctggg cgctcaccgg gctagagccg
     8941 atctttcagc acttcgagcg ggcacagctc acgcgcctgc agtggtacct gctgcagtgg
     9001 atcgagattg agaaccgggc gatgcagttt ctggctgcaa gcggcaagca ggccaattgc
     9061 ttcacgctcc actcgcccca cgaattgagc gacgccggcc gcgtgcggga gctgttcgag
     9121 tttcttggct tgagcctgaa gcacaaaacg ctcgacctcc gcggccggca gaaccgcaac
     9181 ccgcggccga cggtgattac cgatgacgac cgccgtgagc tagccgatgt cgtcggccgg
     9241 ttgccgcccg agcacctcgc catcttccag cgcgagccgt atgtgcgttt tccctggatc
     9301 gagtcgctgc gcttgggtgg cactggcgtt tcgcaagtgt ctgggcgggt cttttgagta
     9361 tcggttaccc tgcgaaccaa cccggcctgc gaattggcct catcgagaat atcccgtttg
     9421 agcggaagcc cgggcgctcg ctcttttccc cttcgatcgg cactggcgac acgccagtgc
     9481 cacccctcta ctcgccggcc tcgatggcgg ccagcagccg gcggcggatt tcggcgaggc
     9541 gcacttcgtc gtcgatcttg cggcccgtgt gctggtcgct gacgtagaac acatcgacga
     9601 cctggtcgag gtgcgtgccg atcttggcct tgctgaccga caggcccagc tcgaacaag
//
LOCUS       DASRJB010000035         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3397654, whole genome shotgun sequence.
ACCESSION   DASRJB010000035 DASRJB010000000
VERSION     DASRJB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397654"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFB80_00930"
     CDS             complement(<1..342)
                     /locus_tag="VFB80_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HZN32341.1"
                     /translation="MSSAVQLDAILPTAIGGSIVRTEGLAAAVAGLPAPVGAVVEIER
                     LSGRPVEAEVIGFRGRETIVFPLGEMAGVRHGHRVRLTRTRRMLRVGPELLGRVIDAR
                     GQAIDHLAPPVL"
     gene            complement(344..1000)
                     /locus_tag="VFB80_00935"
     CDS             complement(344..1000)
                     /locus_tag="VFB80_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HZN32342.1"
                     /translation="MAGIIKAASSSNVATSEPARAFQFDDMGQSYLGNVKAEAARIIS
                     EARQEAARIKAKATEEGKQAAIAAVEASLRTRLEQQLKSGLAALAQASQKITESRHVW
                     QRHWETHAVRLAAAIAERLCRRELARDPAITLTWVREALELAAGNAQLALRLNPGDHA
                     ALATQIERISQQIAGLAPVQIVADEAITPGGCRVETQFGSLDQQLEAQLARLTDEMLA
                     "
     gene            complement(1022..2026)
                     /locus_tag="VFB80_00940"
     CDS             complement(1022..2026)
                     /locus_tag="VFB80_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HZN32343.1"
                     /translation="MPPLSPTLRKAAILISALDDRTADALLDQIGPQDAAKVRSALVA
                     AGDIPADEQEAVLAEFLQTKGGAAADDVALEIDPARPESAPQHAAGPTHAESTAAGPL
                     AFLREVAPQALARVLCTEQPQAVAVVVSSLSAELAAELLERLPQELATDALERLAWLE
                     EPAAEALADLARTLRQQLGPHLGSAAANRQSRAHQNAIRTALAARRRQVGPALAVSIE
                     RYRLESAVESVQPAGLVEFNELAQFDEESLRRVFAAADPQLALVALTGADERLVARIL
                     RSLPSGDAAVLRQRLEHPGPIRLREIEHARQSLSAIAARLARAGEITLPQSIRFAAAA
                     "
     gene            complement(2033..3709)
                     /locus_tag="VFB80_00945"
     CDS             complement(2033..3709)
                     /locus_tag="VFB80_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32344.1"
                     /translation="MDFINKAWLQLAELFRSMSVGARVATAALLALVVMSFVYLFQYQ
                     VTGGDEFLLNGRPFSTSELKEIEAAFAKAGLGQSTVVGNCIRIPRGQKEVYLAALADG
                     NALPADFHNYFDQATTGDSPFASKQSLDLKRWNAKQKELALIIGKMHGIESATVQYDE
                     EVKRGLVQEKRQTAMVAVQSAGRLDEDQIRSIRNIVASAYAGLDRRQITITDLATNQT
                     HGGAIGPDGVPEDESLYASHKQKYERSWQEKISQQLSMIPGVVVGVNVELSPEIEHTS
                     DLLKLDPKPVTVLAGEYSKDSTIQGPGNGGRPGAQSNGVHGNTPVNLNLTASNGPQSQ
                     TTESRSNTHNVPGFEHQVQRNAALVPTRVTASIEVPASYYVSIWKQRNSQPEGQPPKK
                     PDPAELATIETETKNRIKETVHNLLPRVIQGTDPWPHINVQTYTDMPIVAAAAPTVAK
                     TATTWLAGNWQTLALVGLGLASLAMLRGMVRQAAGLPPAAPAAVSEEPRPRLTIHEPP
                     AAEEDPEPSRALKRRFTATGPDLKTELQEIVKENPDAAATILRAWIGEAV"
     gene            complement(3730..4032)
                     /gene="fliE"
                     /locus_tag="VFB80_00950"
     CDS             complement(3730..4032)
                     /gene="fliE"
                     /locus_tag="VFB80_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZN32345.1"
                     /translation="MTPVAGLPIAPPPLPTIGGQQPNLPAGAQPFKNVLVEALEQVNA
                     MQSQANQAVEQLVTGGDVNPAEVLTTLQKADMSFRMMLQIRNKLLQAYQEINNIRI"
     gene            complement(4049..>4289)
                     /locus_tag="VFB80_00955"
     CDS             complement(4049..>4289)
                     /locus_tag="VFB80_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZN32346.1"
                     /translation="IGVKVANVETEQVEPNYRHDPNHPYAIKSGPKKGYVAYPNINMT
                     AEMVDALVATRAYEANLGAIELSKNMAQQSLRILG"
BASE COUNT          613 a         1387 c         1456 g          833 t
ORIGIN      
        1 cagcacgggc ggcgccaagt ggtcaatagc ctggccgcgg gcatcgatca cgcggcccag
       61 cagctccggc ccgacgcgca gcattcgccg cgtccgcgtc aatcgcacgc ggtggccgtg
      121 tcgcacgccc gccatctcgc ccaacggaaa gacgatcgtt tcgcgcccgc ggaagccgat
      181 gacttctgcc tccactggcc gtcccgacag ccgctcgatc tcgacgacgg ccccgaccgg
      241 tgccggcagc cccgcgacgg cggcggcgag cccctcggtg cggacgatgc tgccgccgat
      301 cgccgtcggc agaatcgcgt cgagttgtac ggcgcttgac atgctaggcg agcatttcat
      361 ccgtgagtcg ggccagctga gcttcgagtt gctgatcgag cgaaccgaac tgcgtttcga
      421 cccggcagcc gccgggcgta atcgcctcgt cggccacaat ctgcaccggc gccaggccgg
      481 cgatttgctg gctgatgcgc tcaatctgcg tggccagcgc tgcgtggtcg cctggattca
      541 gccgcaaggc gagctgggcg ttgcccgcgg ccagctccag cgcttcgcgg acccaggtca
      601 acgtgatcgc cggatcgcgg gccagctcgc gtcggcagag ccgctcggcg atcgctgcgg
      661 cgaggcgcac agcatgggtt tcccagtgcc gctgccagac atgacgcgac tcggtgatct
      721 tctgcgacgc ctgggccagc gcggccaggc cgcttttcag ctgctgctcg aggcgagtgc
      781 ggagcgaggc ctcgacggct gcgatcgccg cctgtttgcc ttcttcggtc gccttggctt
      841 tgatccgcgc ggcctcctgg cgggcttcgg agataattct ggccgcctcc gctttgacgt
      901 tccccaggta cgactgcccc atatcgtcga actggaacgc ccgcgccggc tcgctcgtcg
      961 cgacgttgga gctgctcgcg gctttgatga ttccggccat gggatcggga cttctcggcg
     1021 gctaggcggc tgcggcgaaa cggatgctct gcggcagcgt gatctcccct gcgcgtgcca
     1081 gccgcgcggc gatggctgag agcgactgcc gcgcgtgctc aatctcccgc agccggattg
     1141 gccccgggtg ctccagccgc tgccgcagca cggcggcgtc gccgctgggc agcgagcgca
     1201 ggatgcgcgc cacgaggcgc tcgtcggcgc cggtgagcgc caccagggcc agctgcggat
     1261 cggctgcggc aaagacgcgg cggagcgatt cttcgtcgaa ttgcgccagt tcgttgaact
     1321 caacgagccc cgctggctgt acggactcca ctgccgactc cagtcgatag cgttcgatgc
     1381 tgacagccag cgcggggcca acttgccgtc ggcgggccgc gagggcggtg cggatagcgt
     1441 tctgatgggc gcgcgactgg cgattcgcgg ccgcactgcc aaggtgaggc ccaagttgtt
     1501 gtcgcagcgt acgtgccagg tcggcgagcg cttcggcggc cggctcttcg agccaggcta
     1561 gccgctccag ggcgtcagtc gccaattcct gcggcagccg ctccagcagc tcggccgcta
     1621 attctgccga caggctcgac acaacgacgg ccaccgcctg cggctgctcg gtgcacagca
     1681 cgcgggccag ggcctgcggc gcgacctcgc gcaggaatgc cagcgggcct gctgcggtgg
     1741 attcggcgtg cgtcggtccc gcggcgtgct gcggcgcgga ttcaggccgt gccggatcga
     1801 tctccaaggc cacgtcgtcc gctgctgccc cgccttttgt ctgcagaaat tcggccagca
     1861 cggcctcttg ttcgtcggcg ggaatgtcgc ccgccgcgac caaagcgctg cgtaccttgg
     1921 cggcgtcctg cggaccgatc tggtcgagca gcgcgtcggc cgtgcggtcg tcgagcgccg
     1981 aaatcaggat ggcggccttt cgcagtgtgg gggaaagtgg gggcatcgca ggttacaccg
     2041 cctcgccgat ccaggcgcga aggatcgtcg cggcggcgtc ggggttctcc tttacgatct
     2101 cttgcaactc ggtcttcaaa tcgggtccgg tcgccgtgaa tcggcgcttg agcgcccgcg
     2161 acggttcggg gtcttcttca gcggcgggcg gctcgtggat cgtcaagcgc ggccgcggct
     2221 cctcgctgac cgcggcggga gcagccggcg gcaggccagc cgcctgccga accatgcccc
     2281 gcagcatggc caggctcgcc aggcccagtc cgaccagggc tagcgtctgc cagttgccgg
     2341 ccagccacgt ggttgcggtt ttggcaactg tgggcgcggc ggcggcgacg atcggcatgt
     2401 cggtgtaggt ctgcacgttg atgtgcggcc agggatcggt gccctgtatc acccgcggca
     2461 gcagattgtg caccgtctcc ttaatgcggt tcttcgtctc ggtttcgatc gtggcgagct
     2521 cggccgggtc gggcttcttg ggcggctggc cttccggctg cgagtttcgc tgtttccaaa
     2581 tcgagacgta gtagctggcc ggcacctcaa tcgacgcggt gacgcgcgtc gggacgaggg
     2641 ccgcgttccg ctggacctgg tgctcgaacc ccggcacgtt gtgggtgttg gaacgggact
     2701 cggtcgtctg agactggggg ccgttggaag ccgtcaggtt caaattcacc ggcgtgttgc
     2761 cgtgaacgcc gttgctctgg gcgccgggtc gcccgccgtt ccccggcccc tggattgtcg
     2821 agtccttcga atactcgccg gccaaaaccg tcacgggttt ggggtcgagc ttgagcaggt
     2881 ccgaggtgtg ctcgatctcg gggctgagct cgacgttgac gccgacgacg acgccgggaa
     2941 tcatcgacag ctgctgggag attttctcct gccaggagcg ctcgtatttt tgcttgtgcg
     3001 aggcgtagag gctttcgtct tcggggacgc cgtcggggcc gatcgcgccg ccatgggtct
     3061 ggttggtggc gaggtcggtg atcgtgatct gccggcggtc gaggccggca taggccgagg
     3121 ccacgatgtt gcggatcgag cggatctggt cttcgtccag acggccggcc gattgcacgg
     3181 cgaccatcgc cgtctggcgt ttctcctgga cgagtccgcg cttgacctct tcgtcgtact
     3241 gcacggtcgc cgattcgatg ccgtgcatct tgccgatgat gagggccagc tctttttgct
     3301 tggcgttcca gcgcttcagg tcgagggact gcttgctggc gaacgggctg tcgccggtgg
     3361 tcgcctgatc gaaatagttg tgaaagtccg cgggcagcgc gttgccgtcg gccagcgccg
     3421 ccaggtagac ctctttctgg ccgcgcggaa tgcggatgca gttgccgacg accgtgctct
     3481 ggccgagtcc cgccttggcg aacgcggcct cgatctcctt caattcgctg gtcgaaaacg
     3541 gccggccgtt cagcaggaac tcatcgccgc cagtgacctg gtattgaaac agatagacga
     3601 agctcatcac taccagcgcg agcagcgccg ctgtcgccac acgcgcgccg acgctcatcg
     3661 agcggaacag ctcggccagc tggagccagg ctttattgat aaagtccatc cgtggaccct
     3721 cgcttcaact caaatccgga tgttgttgat ctcttgatag gcctgtaaca gcttgttgcg
     3781 gatttggagc atcatgcgga atgacatgtc ggctttctgc agggtcgtca gcacttccgc
     3841 cggattcacg tcgccgcccg tcacgagctg ttcgacggcc tggttcgcct ggctctgcat
     3901 ggcgttgacc tgctccaagg cctccaccag cacgttcttg aagggctgtg ctccggcggg
     3961 caaattcggc tgctgcccgc cgatggtggg cagcggcggc ggcgcgattg gcagtccggc
     4021 aactggagtc atttcataac ctcatcgctt agccgagaat tcgcagcgat tgctgggcca
     4081 tattcttgct cagctcgatg gcacccaaat tggcctcata cgcgcgagtt gccacgagtg
     4141 catcaaccat ctcggcggtc atattgatat tgggataggc cacatatcct ttcttgggcc
     4201 cgctcttgat ggcataaggg tgattggggt cgtggcgata attgggctca acctgttccg
     4261 tctccacgtt ggccactttg acgccgatg
//
LOCUS       DASRJB010000036         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3115792, whole genome shotgun sequence.
ACCESSION   DASRJB010000036 DASRJB010000000
VERSION     DASRJB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115792"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFB80_00960"
     CDS             complement(<1..849)
                     /locus_tag="VFB80_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN32347.1"
                     /translation="MAKSILSRYLDPDVLSVVADRHLDPKGLVIGNLAGAHKSPLAGF
                     AVEFAGHREYVPGDDPKHLDWRVYFTRDKYFVKQYELETNFVCHLVLDVSASMRYGDA
                     REQKLLYAAQMASTLGYSVIRQSDKVSLFTFDDRVRGIVTPSNSMSQVVRLTEHLDQI
                     DPVEKTNMPACLNEIAGRLGRREIVMIFSDFFTNVDELEPVLQRLRYARHEVVLFHVM
                     HHDELAFELDGMVKFVGLEDADEFLAQTDDLRRGYLQAVRQFSERLQEIVHRNGCERV
                     ICDTSRG"
     gene            complement(879..1277)
                     /locus_tag="VFB80_00965"
     CDS             complement(879..1277)
                     /locus_tag="VFB80_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32348.1"
                     /translation="MTSSLRVRWRFSLGSLLALVSVVAVCAALYAARHRALQAQERAF
                     RAIPAKGGLILVYPEGTQIILHPQYVVCAERATKVINPIEKPTDFTDADLHLLDHIIG
                     LNHVNFAGSAVTEKGQAEFTSRHKNCHTAQ"
     gene            complement(1287..2315)
                     /locus_tag="VFB80_00970"
     CDS             complement(1287..2315)
                     /locus_tag="VFB80_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN32349.1"
                     /translation="MTEKTPPDDIAAVQRCEQAYNRLRDELSKVIVGQNEVIEQVLVA
                     MFARGHALLEGVPGLAKTLLISSLAKSLHLTFKRIQFTPDLMPSDVTGTEVIQEDPET
                     RQRGYKFLPGPLFANMLLADEINRTPPKTQAAMLEAMQERQVSAGGTMHQLPAPFFVL
                     ATQNPLEQEGTYPLPEAQLDRFLLYIKVDYPSASEEWEVARRVTTGQLGEIEAVLSGE
                     EILQMQKLVTRVPVSDQVLGYAWALVRATRPGQKEAPEFVNRWVSWGAGPRGVLTLVT
                     CAKARAILYGRYHATVADVQAVAKPALRHRLNGNYAAQANGLNSEKLIDMLFEAVPAD
                     RKYEKPAA"
     gene            complement(2425..>3265)
                     /locus_tag="VFB80_00975"
     CDS             complement(2425..>3265)
                     /locus_tag="VFB80_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32350.1"
                     /translation="GKPSPQQIAAAQALQQAQRQFANAQQMTGEGAAEVSGQQEVANQ
                     PIREGLETASRLNQPLPGEPMPGDGTSPPGSPGEGQPMGQPMPPQGQGQGQPMPGQGQ
                     GQPMPGQGQGQGQPMPGQGQGQPMPGQGLGTGLVPNSPEVTAQQIAGPQANAAAAAAM
                     AAAAQAAQAQGQGQPMPGQGQQPMPGAAQTASKGGAVKGPPSQNPDQAKGELQVEPAA
                     DADSRGSLANQDAAAGGLKFKDEPWFAKLPPSLQAAIQAKARGKAPRGYEERLRRYFE
                     SVD"
BASE COUNT          511 a         1099 c         1040 g          615 t
ORIGIN      
        1 gccgcggctg gtatcgcaga tgacccgctc gcagccgttg cggtggacga tctcctgcag
       61 cctttcggag aactgccgca cagcctgcag atagcctcgc cgcaagtcgt cggtctgggc
      121 gaggaattcg tcggcgtctt ccagtcccac gaacttgacc atgccgtcga gctcaaaggc
      181 cagctcgtcg tggtgcatga cgtggaacag cacgacctcg tgccgggcgt accgcagccg
      241 ctggagcacg ggctccagct cgtccacgtt ggtgaagaaa tcgctgaaga tcatgacgat
      301 ctcgcggcgg cccaggcggc cggcgatttc gttcaggcag gccggcatgt tcgttttctc
      361 gaccgggtcg atctggtcga ggtgctcggt cagtcgcacg acctgcgaca tcgagttgct
      421 gggcgtgacg attccgcgga cgcggtcgtc gaacgtgaac agcgagacct tgtcgctctg
      481 ccggatcacc gaatagccga gcgtcgaggc catctgggcg gcgtagagca gcttctgctc
      541 gcgggcgtcg ccgtagcgca tcgacgcgct gacgtcgagc accaggtggc agacgaaatt
      601 cgtctccagc tcatactgct tgacgaaata cttgtcgcgc gtgaaataga ctcgccaatc
      661 caggtgcttg ggatcgtccc ccggcacgta ctcgcgatgc cccgcgaact ccaccgcgaa
      721 ccccgccagc ggcgacttat gcgcccccgc caggttgcca atcaccagcc ccttcggatc
      781 caaatgccga tccgccacga cgctgagcac gtcgggatcg agatagcggc tgaggatgga
      841 tttggccatg cgatgcttaa ggcgaaatct agactgtgtt attgcgcggt atggcagttc
      901 ttatgcctgc tggtgaattc cgcttggcct ttctcagtta ctgccgagcc ggcgaagttg
      961 acgtgattca agccgataat gtggtccagc aagtgcaggt ctgcgtcagt aaaatcggtt
     1021 ggcttctcta ttgggttgat gactttggtc gctcgctcgg cacacacgac atattgcgga
     1081 tgcaaaatga tctgcgttcc ctcgggatac accaagatca agccgccctt tgcgggaatt
     1141 gcgcggaagg cacgctcctg agcttgcaga gcgcgatgac gcgcggcata caacgcggcg
     1201 cagaccgcaa caacagacac caaagccagc agggagccca agctgaaccg ccagcgaact
     1261 cgcagcgaac tagtcatggg aagacctcac gccgccggct tctcgtactt ccgatccgcc
     1321 ggcacggctt cgaacagcat gtcgatcagc ttttcgctgt tgaggccgtt cgcctgggcg
     1381 gcgtagttgc cgttgaggcg gtggcggagg gcgggcttgg cgacggcttg cacgtcggcc
     1441 accgtggcgt ggtagcggcc gtagaggatc gcgcgggcct tagcgcaagt cacgagcgtc
     1501 agcacgccgc gggggccagc gccccagctg acccagcggt tgacgaactc cggggcctct
     1561 ttctgccccg gccgcgtggc ccgcaccagc gcccaggcat agcccagcac ctggtcgctc
     1621 accggcacgc gcgtgaccag cttctgcatc tgcaggattt cctcgcccga gagcaccgcc
     1681 tcgatctcac cgagctgccc cgtcgtcacg cggcgggcga cctcccactc ttcggaagcg
     1741 ctcgggtaat cgaccttgat gtagagcagg aagcggtcga gctgggcctc gggcagcgga
     1801 taggtgcctt cctgctccag cggattctgc gtcgccagca cgaagaatgg ggccggcagc
     1861 tggtgcatcg tgccccccgc gctgacttgc cgctcctgca tcgcctccag catcgccgcc
     1921 tgcgtcttgg gcggcgtgcg gttgatctcg tccgccagca gcatgttggc gaagagcggc
     1981 ccgggcagga acttgtatcc gcgctggcgc gtctccggat cctcctgaat cacctcggtg
     2041 ccggtcacgt cgctgggcat caggtcgggc gtgaactgaa tccgcttgaa agtcaggtgc
     2101 agcgacttgg ccagcgagct gatcagcagc gtcttcgcca gccccggcac gccttccagc
     2161 agcgcgtgcc cgcgggcgaa catcgccacc agcacctgct cgatcacttc gttctgcccg
     2221 acgatcacct tcgacagctc atcgcggagg cggttgtagg cctgctcgca ccgctgcacc
     2281 gcggcgatat cgtcgggtgg ggtcttctcg gtcattgcga tctccaaatt cggtagggaa
     2341 cgccaactgt ggcgttcccc gatctgcaac aaaaacgcaa ttgtcggttc cggcacgcca
     2401 cagagggcgt gccctaagtt gccgttagtc aacactctca aaataccgcc gcagccgctc
     2461 ttcgtagccg cggggcgctt tgccgcgggc cttggcctgg atggcggcct gcaagctcgg
     2521 cggcagcttc gcaaaccacg gctcatcctt gaatttcagc ccgcccgccg ccgcgtcctg
     2581 attggccagg ctcccccggc tgtcggcgtc ggccgccggt tcgacttgca actcgccctt
     2641 ggcctgatcg gggttctggg agggcggacc cttcaccgcg ccgcccttgc tcgccgtctg
     2701 ggcagcgccc ggcatcggct gttggccctg gcccggcatc ggctggccct gaccctgtgc
     2761 ctgcgctgcc tgcgcggcgg cggccatcgc agcggccgcg gcggcgttcg cctgggggcc
     2821 ggcgatctgc tgcgccgtca cctccggcga attcggcacc aggccggtgc ccaaaccctg
     2881 gccgggcatc ggctgacctt gaccctgacc cggcatcggc tggccctggc cttgcccttg
     2941 tccaggcatc ggctgcccct gaccctgtcc cggcatcggt tgaccttgcc cctgtccctg
     3001 gggcggcatt ggctgcccca tcggctgccc ttcacccggc gagcctggag gcgacgttcc
     3061 atcgccaggc atcggctcgc ctggcagcgg ctgattcagt cgtgaggcgg tttcgagacc
     3121 ctcgcggatg ggctggttcg cgacctcctg ctggccggag acttcggccg caccctcgcc
     3181 agtcatctgc tgggcgttgg caaactgtcg ctgagcctgc tggagggctt gggcggcggc
     3241 gatctgctgc ggcgacggct tgccc
//
LOCUS       DASRJB010000037         7487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_860605, whole genome shotgun sequence.
ACCESSION   DASRJB010000037 DASRJB010000000
VERSION     DASRJB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860605"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFB80_00980"
     CDS             complement(<1..1236)
                     /locus_tag="VFB80_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZN32351.1"
                     /translation="MTVPAVEGARTAAGEDRLLRLRRWLGLVGAPLAFGIVLACRFPG
                     LTPPAHRLAAIVAAVMVLWISEALPLPVTALAAAAACILLQVAPAKDVFLPFADPLIF
                     LFIGAFILARALFLHRLDRRLAFWVLALPWVGARPGRVLVALGAVTAFISAWVANAAT
                     TAMMYAIAISLIHSLADSRRGRPLVQPAYATGLLLMTTFAASVGGLATPVGAAPNLIG
                     LGFIRNRLGVNFSFLDFCTVGAPAAAVLFLFVAGYLALLCPAGVREIEGGRELFRSER
                     SKLGPWTWGQRSTLVACTLTIGLWLAPAALALTLGEQSPLYQYVDDAVPEAVAALLGA
                     VLLFLLPGDLLATGERGRAISWEDAARIDWGVILLFGGGLSLGLLASQTGLAQAIGRD
                     LAAVLPAHSPLGLVVLATVT"
     gene            complement(1233..2174)
                     /locus_tag="VFB80_00985"
     CDS             complement(1233..2174)
                     /locus_tag="VFB80_00985"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN32352.1"
                     /translation="MLTETIADQRAWTRATAGRPDHWYRPLPPGLAAALAEEVDRAPA
                     AQPLTELRLSDELHRTWEGSLAPARRDLAAGPGFVILEGLDGLPARTATGCYWLLGQL
                     LGQPFAQNVQGTLLYDVRDTGQDVAAGARFSVTSYESSYHTDNSFGDGVLDYVGLLCL
                     QTAKAGGLSQNVNAYAVHNVLLCEQPAALALLYQPWHVDRRGGFREGESPTVRRPIIT
                     WDGRELTVRYLRYWIEAGHEKAGQPLTADERRALDIFDAVLARPELRIEFALRPGEAY
                     FINNRWILHNRTAFTDYAEPERRRHLVRLWLRAGDGA"
     gene            complement(2186..3307)
                     /locus_tag="VFB80_00990"
     CDS             complement(2186..3307)
                     /locus_tag="VFB80_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN32353.1"
                     /translation="MPRLFATCLFVFFVAILPTAAQDDAQKAKDALIVRTLLRLPGID
                     LSAKPEAKAALLRHLETLKGTDQYVEIVEKFRLSETKEELLRLAVEKGDSSLGVKAAG
                     LLVKLDQKDAITKAIADPDPAKAAKFITALGLLADAKINDWLEPLVSDVQNPAATRTA
                     AVTALGRNAPGQKWLLATVEQGKLPEDCKFAAANALLASADEAIRTAAARHLSLPAVA
                     GGEPLPPIAELVKRRGDAARGRELFFGMTATCAKCHKVKGEGKEVGPDLSEIGTKLSK
                     EALYLSILDPSAGVSFNYETWLVRDFEGTILTGVLVSQTDAEIELKTAEAISHKLKRS
                     DIEQLKKSPLSLMPADLQKQLQAQDLVDIVEYLAALKKE"
     gene            complement(3340..6810)
                     /locus_tag="VFB80_00995"
     CDS             complement(3340..6810)
                     /locus_tag="VFB80_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN32354.1"
                     /translation="MRFWFVFSAVVVSLIPASTRAADEAKTIVFVAGRPSHGYGAHEH
                     NAGCTLLAHELRSALPKYTCHVHLNGWPQDKAAFDKADCIIMYCDGGGGHMVNAHLDE
                     VDELAKKGVGIVCIHYGVEVPKGKSGDKFLDWIGGYFEMNWSVNPHWTAKYENLPDHP
                     ICRGVQPFEINDEWYYHMRFREGLKGVTPILTALPPKETLSRGDGTHSGNPAVREAIA
                     KGEPQHMAWAAEREGGGRGFGFTGGHDHWNWGDPNFRKVVLNAIVWCAKGEVPSGGVE
                     SPVVTLKALEANMDPKNKGNFNRESIRQKLKLPPDEPAAGGAASAAPAANQAAEAQPA
                     ASNTAQPAARNTEQPRRGQRGKGGPSRPTYSSPLVSSATKNHSVEIDIDITGARRLWL
                     VVNDNGDGFGCDWADWAEPRLIGPSGEKKLTEISWKSATAGYGNVFVNKRCDGKEGLV
                     INGQKVEYGIGTHANSIIQFDLPQEGGYTRFKARGGLDEGGTRQGCGSTVQFQVYLNQ
                     PPEGVGRASGAVVAGSDSPVHAAASAVTNLDVAEGCEAALFASEPDISNITNIDIDHL
                     GRVWACEVKNYRKWNGSRPEGDRILVLEDTDRDGKCDKQAVFYQGRDIDSAHGVCVLG
                     KQIIVSAGDKVQVFYDENGDLKADAKRSLLFTGISGTQHDHGIHAFVFGPDGKLYFNF
                     GNSGNQLKDKDGKPVVDKAGNAVAANRKPYQQGMVFRCNLDGSQVETLGWNFRNNWEV
                     AVDSFGTLWQSDNDDDGNRGVRINYVMEFGNYGYVDEFTGAAWKTPRENMEKEIPQQH
                     WHLNDPGVVPTLLLTGAGSPTGICVYEGALLPKVFHGQVIHCDAGPSVCRAYPVTKDG
                     AGYKAETVDILSGARDNWFRPSDACVAPDGSLFVADWYDPGVGGHNQQEVDKGRIFRV
                     APPGAKYSVPIYDFATPAGCIQALASANLEARYLAFTGLMSQGDKAHHPVEEAIEKGT
                     EPRLKARLLWVLAQIPGEGEEAIELALADGDDQVRVTGVRIARQLGQDIAELSGKLAK
                     ESSKQVQRELAIGLRHSKSPKAPAAWAELAVQYDGQDRWYLEALGLAADKQWDAYLDA
                     YLSKTSEPWKTPAGRDILWRSRAKKTPELLVKIMKDPATKESDQPRYFRAFDFLNGPE
                     KEAALKSLLE"
     gene            complement(6891..>7487)
                     /locus_tag="VFB80_01000"
     CDS             complement(6891..>7487)
                     /locus_tag="VFB80_01000"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZN32355.1"
                     /translation="PLAGGRTALTIADVSGHGLGPSLLMASVRAALRTQLLDDSNPQS
                     LLEHLARALADDFEHGAFITMFLGLLDPTKHELQHSNAGHGPVLVYRPAARQFDELKS
                     TGVPLGVIVPTDYPLAPPVAFAPGDLLILVTDGIVESMDKRGEQFGVERLKSLVAKQH
                     ALPVQELARQIGRQVELHYVGDSPPDDLTVLLLRRTGP"
BASE COUNT         1177 a         2536 c         2494 g         1280 t
ORIGIN      
        1 ggtgacggtc gccaggacga ccaggcccag cggcgagtgc gccggcagga ccgcggccag
       61 atcgcggccg atcgcctggg ccaggcccgt ttgcgaggcc aagaggccca ggctcaggcc
      121 gccgccgaag agcaggatca cgccccagtc gatccgcgcc gcgtcttccc agctgatggc
      181 ccgcccccgt tcgcccgtcg ccagcaagtc gcccggcagc aggaagagaa ggactgcgcc
      241 cagcagcgcc gccaccgctt ccgggaccgc gtcgtccacg tattgataga ggggcgattg
      301 ctcgccgagc gttagcgcca gcgcggctgg tgcgagccag aggccaatcg tcagcgtgca
      361 ggcgacgagc gtggagcgct ggccccaagt ccatggtccg agcttgcttc gctccgagcg
      421 aaacagctcg cggccgcctt cgatctcgcg gacgccggcc gggcagagca gcgccagata
      481 gccggccacg aacagaaaca gcaccgccgc cgccggcgcg ccgacggtgc agaagtcgag
      541 aaacgagaag ttcacgccca gccggttgcg gatgaagccc agcccgatca ggtttggcgc
      601 ggccccgacc ggcgtggcca gcccgcccac cgaggcagca aaggtcgtca tcagtagcag
      661 gcccgtggca tacgccggct gcacgagcgg ccggccgcgg cgggaatcgg ccagggagtg
      721 gatcagtgag atggcgatgg cgtacatcat ggccgtcgtc gccgcgttgg cgacccaggc
      781 cgagatgaac gccgtcaccg cgcccagcgc gaccagcacg cgtcccggcc gcgcgccgac
      841 ccacggcagc gccagaaccc aaaacgccaa ccggcggtca agccggtgca ggaacagggc
      901 ccgcgccagg atgaacgcgc cgatgaacag gaagatcagc ggatcggcga agggcaggaa
      961 cacgtccttg gccggcgcga cttgcagcag gatgcacgcc gccgcggccg ccagcgccgt
     1021 caccggcagc ggcaacgctt cgctgatcca aagcaccatg accgcagcca caatcgccgc
     1081 cagccgatgc gccggcggcg tgaggccggg aaagcggcag gccaggacga tgccgaatgc
     1141 cagcggcgct ccgactagac cgagccagcg ccgcagacgg aggagtcgat cctcgcctgc
     1201 cgcggtgcga gcgccctcca cggccggcac ggtcatgccc catccccggc gcgcagccac
     1261 agcctgacca gatgccgccg ccgttctggt tcggcgtaat cggtgaacgc cgtgcggttg
     1321 tgcaagatcc agcgattgtt gatgaagtac gcctcgccag ggcggagggc gaattcgatc
     1381 cgcagctcgg gccgggcgag caccgcgtcg aagatgtcta gcgcccgccg ctcgtccgcc
     1441 gtgagcggct ggcccgcctt ctcgtggccg gcctcgatcc aataccgcag gtagcgcacc
     1501 gtcaactcgc ggccgtccca ggtaatgatc ggccgccgga ccgtgggcga ctcgccttcg
     1561 cgaaacccgc cccgccgatc gacgtgccac ggctgataaa gcagcgcgag cgcagcgggc
     1621 tgctcgcaga gcagcacgtt gtgcacggcg taggcgttga cgttctggct gagcccgccg
     1681 gccttcgccg tttgcaggca gagcaggccc acgtagtcga gcacgccgtc gccgaaagag
     1741 ttgtccgtgt gatagctgct ctcatagctc gtgacgctga accgcgctcc ggcggccacg
     1801 tcctgcccgg tgtcgcggac gtcgtagagc aacgtgccct gcacgttttg tgcaaagggc
     1861 tggcccagca actggccgag cagccagtag cagccggtcg ccgttcgagc cggcagcccg
     1921 tccaggcctt ccaggatcac aaaccccggg ccggccgcca agtcgcgccg cgccggagcg
     1981 agcgatccct cccacgtgcg gtgcagttcg tcgctcagcc gcaattcagt caacggctga
     2041 gcggcgggtg ctcggtcgac ctcctccgcg agcgccgccg ccaggcccgg cggcagcggc
     2101 cgataccagt ggtctggcct gccggcggtc gcccgagtcc aggcgcgctg gtctgcaatc
     2161 gtctccgtca gcatcgccac gacgctcact ccttcttcag cgcggccagg tactcgacga
     2221 tgtccaccag gtcctgggcc tggagttgct tctggagatc ggcgggcatc aacgagaggg
     2281 gagacttctt cagctgctcg atatcgctgc gcttcagctt atgggaaatc gcctcggcgg
     2341 ttttcagctc gatctcggcg tcggtctggc tgacgaggac gccggtcagg atcgtccctt
     2401 cgaaatcgcg aaccagccag gtctcatagt tgaagctcac gcccgcgctg ggatcgagaa
     2461 tcgacaggta gagcgcctcc ttcgagagct tggtgccgat ctcggataaa tcagggccga
     2521 cctccttgcc ttcgcctttg accttgtggc acttcgcgca ggtcgccgtc atgccaaaga
     2581 acagctcgcg tccgcgggcc gcgtccccgc ggcgcttgac cagctcggcg atcggcggca
     2641 gcggctcgcc accggcgacg gcgggcagcg acaggtgcct ggcggccgcg gtgcggatgg
     2701 cctcgtcggc gctggcgagc agggcgttgg ccgctgcgaa cttgcagtcc tcgggcaatt
     2761 tgccttgctc gactgtggcc agcagccact tctgccccgg agcattgcga cccagcgcgg
     2821 tcaccgccgc ggttcgagtc gcagcgggat tctgcacgtc ggaaaccagg ggctccagcc
     2881 agtcgttgat tttcgcatcc gcgagcaggc ccagcgccgt aatgaacttc gccgccttag
     2941 ccggatcggg atcggcgatg gccttggtga tcgcgtcctt ctggtccaac ttcaccagca
     3001 gccctgcggc tttgacaccg agcgagctgt cgcctttttc cacggccagc cgcaacagct
     3061 cctcctttgt ctcgctcagg cggaacttct cgacgatctc gacgtactgg tcagtcccct
     3121 tgagcgtttc caagtgcctg aggagcgccg ccttcgcctc gggcttggcc gacaggtcga
     3181 tccccggcag ccgcagcagc gtgcgaacga tcagcgcgtc cttggccttc tgcgcgtcgt
     3241 cttgcgcagc ggttggcagg atggccacaa agaacacgaa aaggcaagtc gcgaacaaac
     3301 ggggcatgtg tcagtcacaa tcgagagtgc actcaccact tactccaaca ggctcttcag
     3361 agccgcttcc ttctcgggac cattgagaaa atcgaacgca cggaaatatc gcggctgatc
     3421 gctttccttg gtcgcggggt ctttcatgat cttcaccagc agctcgggag tcttcttggc
     3481 ccggctgcgc cagaggatgt cgcggccggc gggcgtcttc cagggctcgc tggttttgga
     3541 gaggtaagca tcgaggtagg cgtcccactg tttgtccgcg gccagcccca gggcttccag
     3601 gtaccagcgg tcctggccgt cgtactgtac cgccaactcg gcccaagccg ccggggcctt
     3661 gggcgacttg ctgtggcgga ggccgatggc cagctcacgt tggacctgct tggacgattc
     3721 cttggccagc ttgccggaca actcggcgat gtcctggccc agctgcctgg cgatgcggac
     3781 gccggtcacg cggacctggt cgtcgccgtc ggccagcgcg agctcgatgg cttcctcgcc
     3841 ttcaccggga atctgggcca gcacccacag caggcgggcc ttcagccgcg gctcggtccc
     3901 cttttcgatc gcctcttcga ctgggtgatg ggccttgtcg ccctgggaca tcagccccgt
     3961 gaacgccagg tagcgggcct cgaggttcgc gctggccagc gcctggatgc agccggccgg
     4021 cgtcgcgaag tcgtatatcg ggacgctgta tttcgcgccc ggcggggcga cgcggaagat
     4081 gcggcccttg tcgacctcct gctggttgtg gccgccgacg cccggatcgt accagtcggc
     4141 gacgaacagc gagccgtcgg gcgcgacgca ggcgtcgctg ggccggaacc agttgtcgcg
     4201 agcgccggag aggatatcga cggtctccgc cttgtagccc gcaccgtcct tggtcaccgg
     4261 atacgcccgg cagacgctcg ggccggcgtc gcagtgaatg acctggccgt ggaacacctt
     4321 gggcaggagc gcgccttcat agacgcagat gcccgtcggc gagcccgcgc cggtgagcag
     4381 cagcgtgggc acgacgccgg ggtcgttcag gtgccagtgc tgctgcggaa tctccttctc
     4441 catgttctcg cggggcgtct tccaggccgc gcccgtgaac tcatcgacgt agccgtagtt
     4501 gccgaattcc atcacatagt tgatccgcac gccgcgattg ccgtcgtcgt cgttgtcgct
     4561 ctgccagagc gtgccgaacg aatcgaccgc cacctcccag ttgttgcgga aattccagcc
     4621 cagcgtctcg acctggctgc cgtcgaggtt gcagcggaag accattccct gctggtaggg
     4681 cttgcggttg gcggcgaccg cgttgcccgc cttatcgacg acgggtttgc catccttgtc
     4741 tttgagctga ttacccgagt tgccaaagtt gaagtacagc ttgccgtcgg ggccgaagac
     4801 gaaggcgtgg atgccgtggt cgtgctgcgt gccgctgatg ccggtgaaca gcaggctgcg
     4861 cttggcgtcg gccttcaggt cgccgttctc gtcgtagaag acctgcacct tgtcgccggc
     4921 cgaaacaatg atctgcttgc ccagcacgca cacgccgtgg gccgagtcga tgtcgcggcc
     4981 ctggtagaag accgcctgct tgtcgcactt gccgtcgcgg tcggtgtctt ccagcacgag
     5041 aatccggtcc ccttccggcc ggctgccgtt ccatttgcgg tagtttttca cctcgcaggc
     5101 ccagacgcgg ccgaggtggt cgatgtcgat gttggtgatg ttgctgatgt ccggctcgga
     5161 agcaaacagc gcggcctcgc agccctcggc aacgtccagg ttggttaccg cgctggccgc
     5221 ggcgtgcacc ggcgaatcgc tgccggcgac cacggccccg ctggcccgcc caacgccctc
     5281 cggcggctga ttgagataga cctgaaactg caccgtggag ccgcagcctt gcctggtgcc
     5341 accctcgtcg aggccgccgc gggctttgaa cctcgtgtag ccgccctcct gtggaaggtc
     5401 gaattgaatg atgctgttgg cgtgcgtgcc gatgccgtac tcgaccttct gcccgttgat
     5461 gacgagcccc tccttgccgt cgcagcgctt gttgacgaag acgttgccgt agccggccgt
     5521 cgccgacttc cacgagatct ccgtcagctt cttctcgccc gacgggccga tcagccgcgg
     5581 ctcggcccag tcggcccagt cgcagccaaa gccgtcgccg ttgtcgttca cgaccagcca
     5641 caggcgacga gcgccggtga tgtcaatgtc gatctcaacc gaatggttct tcgtagccga
     5701 cgagacgagt gggctggaat acgtcggccg cgacgggccg cccttgcccc gctgcccgcg
     5761 ccgcggttgc tcagtgtttc tggcggcggg ctgcgcagta ttgctagccg cgggctgcgc
     5821 ttcggccgct tgattggccg cgggcgccgc acttgccgcg ccgccagccg cgggctcgtc
     5881 gggcggcagc ttcagcttct ggcggatgct ctcgcggttg aagttcccct tgttcttcgg
     5941 gtccatgttg gcttcgagag ccttgagggt cacgacgggc gattcgacgc cgccggaggg
     6001 cacttcgccc ttggcgcacc agacgatcgc gttgagcacg accttgcgga agttcggatc
     6061 gccccagttc cagtggtcgt ggccgcctgt gaatccgaaa ccgcggccgc ccccttcgcg
     6121 ctccgcggcc caggccatgt gctgcggttc ccccttggcg atcgcctcgc gcacggccgg
     6181 attgccgctg tgggtgccgt cgccgcggct gagcgtttcc ttcggcggca gggccgtgag
     6241 gatcggcgtc acccccttca gcccctcgcg aaaccgcatg tggtagtacc actcgtcgtt
     6301 gatttcgaac ggctgcacgc cgcggcaaat cggatggtcc ggaaggttct cgtatttcgc
     6361 ggtccagtgc gggttcaccg accagttcat ctcgaagtag ccgccgatcc agtcgaggaa
     6421 tttatcgccg ctctttccct tgggcacttc cacgccgtag tggatgcaga cgatgccgac
     6481 gcccttcttg gccaactcat cgacctcgtc gaggtgcgca ttgaccatgt gccctcctcc
     6541 gccgtcgcaa tacatgatga tgcagtcggc tttgtcgaaa gcggccttgt cctgcggcca
     6601 gccgttgagg tgcacgtggc aggtgtactt cggcagggcc gaacgcagct cgtgggccag
     6661 cagcgtgcag ccggcgttgt gctcgtgcgc gccatagccg tggctgggcc ggccggcgac
     6721 gaagacgatc gtcttggctt cgtcggcggc gcgtgtcgag gcaggaatca gggagacaac
     6781 cacggcagaa aagacgaacc agaatcgcat ggcgagctcc aacgcagggg agggacgggc
     6841 agcgaaactc acgatagccg ggcactctgc gagcgtcaaa ccgacgtgcg tcaagggccc
     6901 gtgcggcgga ggagcaggac ggtgagatcg tccggcgggc tgtcgccgac gtagtgcagc
     6961 tcgacctggc ggccgatctg gcgggccagt tcctggaccg gcagggcgtg ctgtttggcg
     7021 accagcgatt tcaagcgctc gacgccgaac tgctcgcccc gcttgtccat cgactcgacg
     7081 atgccgtcgg tgaccaggat cagcaggtcc cccggcgcga aggccacagg cggcgcgagc
     7141 ggatagtcgg tcggcacgat gacccccagc ggtacgccgg tcgatttcaa ctcgtcgaat
     7201 tgccgcgcgg ccggccggta gaccagcacg ggtccgtggc cggcattcga gtgctgcagc
     7261 tcgtgctttg taggatcaag caggcccagg aacatcgtga tgaacgcgcc gtgctcgaag
     7321 tcgtcggcca gcgcgcgggc caggtgctcg agcagcgact gcgggttgga atcgtccagc
     7381 agctgcgtcc gcagcgcggc ccgcacggac gccatgagca ggctcgggcc cagcccgtgg
     7441 ccgctgacgt cggcgatggt cagcgctgtg cggccgccgg ccagcgg
//
LOCUS       DASRJB010000038        11886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3680812, whole genome shotgun sequence.
ACCESSION   DASRJB010000038 DASRJB010000000
VERSION     DASRJB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3680812"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..2432
                     /locus_tag="VFB80_01005"
     CDS             630..2432
                     /locus_tag="VFB80_01005"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZN32356.1"
                     /translation="MPLIVQKFGGSSVASPEKILRAARRAIRAQQEGNQVVMVVSAMG
                     DTTDDLLELAAKITDRPPAREMDMLLSTGEQVSVALMAIAIHSLGHKAVSLTGAQIGV
                     KTDASYRKARIKSIETTRLRKSLDEGHIVIAAGFQGIDDDFNITTLGRGGSDTTAVAL
                     AAVLRADACEIYTDVDGVYTTDPRVVPEARRVGRIGYDEMLELASLGAGVMHSRSIEF
                     GKKFDVPIHVRSSFTDQPGTMIVAEPERPDLPVSGAAITKDEALITVQGVPDRPGTSL
                     EIFARVAARNISVDMIVQNVGESGKADISFTVPRNELDVALEAVREAVKLVGADAITS
                     DDSVAKVSVVGLGMAKQTGVAQKMFRALAAAGINIQMITTSEIKISVLVSRERSQEAL
                     RIVHHAFGLESAAPDVARDSIAAARHGVAGYGNGTDAAAVVARLQSMNMEELTIDGVD
                     LDSSQARVTISGVPDKPGIAAKVFEDVAAAGVFVDMIVQSHPAAAGAAILSFTVPQNQ
                     LTKSVEAARRIAQSLGCQMVSSSPIIAKLSVSGVGLRTHVGVAIRMFKALAEAGINLE
                     MINTSEVRVNVVVDGAAGEKGLKQLQAAFADVLR"
     gene            2661..2900
                     /locus_tag="VFB80_01010"
     CDS             2661..2900
                     /locus_tag="VFB80_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32357.1"
                     /translation="MSPTLATVVEPFVDRRNYAGQAGAPTRERRQFTNSHEDLSPDAR
                     ELAQAIDSYKLVHRRRFITYEEMLSVFKSLGYKRD"
     gene            complement(2897..4105)
                     /locus_tag="VFB80_01015"
     CDS             complement(2897..4105)
                     /locus_tag="VFB80_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN32358.1"
                     /translation="MKRLTFVLAALLLAASALVAWQPAGSQSPAQKATLPGARPAIEA
                     IDYPSLQAAIDALPPTGGVVRLPAGTFEITEPLRISQEDVLIAGSGTATHIKNVNTSG
                     RPAIEIDSPKRKENPRATLWRVEIQNLRVTGNEKSGDGILAHHVDEIFLHGVTSSYHG
                     GHGIYLDQCYEDPRVCDCLLTYNKGAGLHLVVCHDIVVVGNQFEENQDAVHCFDGFNL
                     CMTGNNLDDHLRHGVVIENTYGSVVSGNMIEECNGTAIILDRDCYGVTLSSNVIAHEA
                     GGGIDLRDAHGCAVSGNTFTIVKQDALRIGPASGRITVTGNNFSSSYIGDGKDKRTTA
                     DRSAAGIVLAGTSDIAISGNTFSGLSTKALTLEGDASKRVLFSNNLLTEVQSDHPQLE
                     KAAASGNLDN"
     gene            complement(4145..4828)
                     /locus_tag="VFB80_01020"
     CDS             complement(4145..4828)
                     /locus_tag="VFB80_01020"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HZN32359.1"
                     /translation="MSKSTLLASRRPRKAKSVRNRAAASAAAIYAVHPGIAMVQKWID
                     ELPAKTGRSLDDWLAHIRKAGPRDEKASREWLMSEHRLGANTAWWLAEKAFHPDKLAE
                     DTPAGYLKLAPVFVERMYSGPKEHLRPIHDRLIELARELEGVRICPCETMVPLYRRHV
                     FAQIKPTTNKRVDLGLALAEEPFTSRLLDTGGRAKKDRITHRVALTSLDDIDLQVRRW
                     LKQAYERDA"
     gene            5074..6345
                     /locus_tag="VFB80_01025"
     CDS             5074..6345
                     /locus_tag="VFB80_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32360.1"
                     /translation="MLSIDLGAKSRYCDGISRRSLLKLGALGIGGLTLPGLLRAEAAA
                     GVRSSNKAIINIHLSGGPSHQDMFDLKPEAPREFRGEFNPIKTNVPGMEICEHMPLLA
                     KMADKFAVIRSLVGMFDDHSNFHTQTGFYRDNLRNVGGRPSIGSVVSKLLGPTGTGAP
                     CYIAYNDGPEGYLGPVHKPYRPQGGDLKLVGGMDETRLSTRTNLLTSLDRIRRDTDGS
                     GKMTALDSFTQRAVGVVTSGKVADALDLEKEDKAVRERYGRDDGSQFLRARRLIEAGV
                     RVVTFDWGGWDTHGDNFNQLRRQLPKLDQGMSALLTDLSERGLDKDVTVVMWGEFGRT
                     PRVNGGAGRDHWSRVAMCFLAGGGMRLGQAIGTSTAYAEEAKDRPVQLQEVFATLYHN
                     LGIDVRRQQLIDPAGRPQYLVEVREPIKELI"
     gene            complement(6364..7251)
                     /locus_tag="VFB80_01030"
     CDS             complement(6364..7251)
                     /locus_tag="VFB80_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN32361.1"
                     /translation="MKTCLFSVSYAGFWGQAALPLTEFIARAGRLGFSSVMIAGKRPH
                     LSPLDATPQLLREVQATLSRSGVTCEVLAAYTNLAPGAAEVPDAEMQIAYVESLARIA
                     AELGAKVVRVFTAYESGGHDPQTLWRRVASALQEMCDRAAQHGVTIAVQNHHDLALDT
                     AALLELLADVGRPNCKLGFDAWSPALRGEEMYAAAKLAAPHTAITTNADYIRVPRHRY
                     RPELVNYERTQPDWARAVPFGTGFIDYRAFFRGLRDGGFDGLATYEICSPIRGGGSLE
                     NLDASAATYLKWLRENELA"
     gene            complement(7248..8411)
                     /locus_tag="VFB80_01035"
     CDS             complement(7248..8411)
                     /locus_tag="VFB80_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32362.1"
                     /translation="MPLVRFSILTLSLLPAIAAAEEPRAAELRKKLVPFTQPPAEFTS
                     QFGDYRSPLVFADGSRVQSPADWARRRSEIVKLWHERLGAWPPLVEKPAVKRLESVEK
                     DGYTQHHVHVQVSPDGKVADGYLLIPKGNRPFPAVLVPFYEPLTSIGAGQKGQGTHDY
                     GLQLVKRGFVTLSIGTPGSVEKIGLETRQLLVEAGKEQRRQPLTLLAYVAANCHTALA
                     QMPEVIPERIGIIGLSYGGKWSMFASCLHEKFACAVWSDPGIVFDEKNSNVNYWEPWY
                     LGFDPNVQRKPGIPSDENPRTGLYKELREAGEDLVSLHALMAPRPVLDSGGTEDPPRN
                     WRALNHLIAVNEVLAHKQRVAMTARKTHVPTPEALEIELAFLEYWLKHALDEK"
     gene            8504..10489
                     /locus_tag="VFB80_01040"
     CDS             8504..10489
                     /locus_tag="VFB80_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZN32363.1"
                     /translation="MNRLNRRELLGLTLASSAGLVAAQQAAPAVDLHRQLLELAARQE
                     RQRRERFAAVKTAAELDALQKKLRADFLDLLGGLPSNDRPPSAHTTRAIEADGYRIEK
                     LVFESFPGYFVPALLYRPTSAGGPRPAVISPCGHSAVGKSAPPYQMLHVNLAMRGFIV
                     LTYDPVGQGERSQFWDAAKGASRFNLVCGEHAVLGNPLYLLGTSLARYRIWDGIRAID
                     YLASLSDVDPKRIGCVGNSGGGTLTAYIAALDSRVAAAAICCYITTLPRRMGNRIQQD
                     PDADPEQDIFGFVSAGIDHAGLLALRAPRPTLVGAALEDFFPIEGARESFAEARRLFE
                     VAGTADRIALAEAPGRHGLSLTLRNAVLRWFDRWLAGGKGDESIVEAPVEPRPAADLQ
                     VTESGQVNVSLQSRPLLPLAWEQFRAQPIAQRLPLREIVQPDVDSASFRLAELSAAKP
                     GNSTVVVLINGNESPDWREQKELLTALGGRGHAVVSLDPRGVGALRPKELRVGGRDYA
                     DPLAGVEENLAYNAFLVGKSLLGIRVSDVLAAVRELSAKSPRIVLCGRRDSALVACFA
                     AALEPKVHGVAVQEMPLSFLPLFSTEGTPINAASILPGVLRSFGDLPDVLAQIAPRKL
                     LVAGATGQRPQGLSGASFASEPFTKNSQLLLDWLAAG"
     gene            10580..>11886
                     /locus_tag="VFB80_01045"
     CDS             10580..>11886
                     /locus_tag="VFB80_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32364.1"
                     /translation="MLRLLLTSSLAMIVAGAGLPPSGRGGDWPQWRYDAQRSAASPDE
                     LPAQLRTHWVRELPRLKPAWPDQAKMQFDAAYEPVAAGQRLFVGSSFDNTVTAYDTRS
                     GRELWTFFADGPVRFAPLVWEDKLYFACDDGYLYCLKAADGSLIWRFRGGPSDRKILG
                     NERLISTWPARGAPVVADGKVYFAAGIWPFMGIFLHALDARTGEVIWTNDGDGSTYMK
                     QPHNADSFAGVAPQGPLAVVGERLLVPGGRSVPACYDRATGKLIYYQLAENAKKGGGS
                     AVAATDQLLFNGRAVFDLATEKYLGSAGEFVAFAGGRLYDGDEGKLREIDLQASGLSQ
                     EKSIDRTGKSATAAKWSIKQLGQAGTPKVTALIKAGSRLYVGSSGRVLAFALPLDTEG
                     DAKPIWEAELEGTPVSLVAADDRLFATTLEGRLYCFGVADRAPS"
BASE COUNT         2042 a         3813 c         3957 g         2074 t
ORIGIN      
        1 aagacgccac ctcccccggc ctcacccctc ggcatgccct ccatgccgtg agtcaattcc
       61 gccgcttggc ggaattgtga acattggtac ggtatttgca aaaaagtccc atttggcgca
      121 gcgggcctgt cattttggca gaaggtcgag ttcgcatctt gttgcgagtg ccaccagctg
      181 aggcggcagc aaatgtgcaa tatataactt gttaacgggt tggcgggtga tcagatgcaa
      241 gtcgatacgt cgtccaatct tgcggcgaaa tcggcgcaga aaggccagtt catacttacc
      301 tatccaggtg gtgcgtatgc ggcagggacc tccaacccca ttgggccgcg acggcagtgc
      361 gggctcccag atgacgccgc gggacattcg ctgaattccg ctgcctaagt tgcattaaac
      421 tgcataaccg acttgcatca tgaatgacac aactcgtgtc acaccaacag tttaaaccga
      481 ttcttgccgg ggagattttt ctttcggtat gcatgcatag tcgacggggc tggacggttt
      541 gcggggacgc agggagatta gcgacgtagc ggcggcttcc agccgcggag cgcggagcag
      601 gacccgctac gagcaaagta aggttggaca tgccactgat cgtgcagaag tttggcggct
      661 cttcggtcgc cagccccgag aaaatcctgc gggcggcgcg gcgggcgatc cgcgcgcagc
      721 aggagggcaa ccaggtcgtg atggtcgtca gcgcgatggg cgatacgacc gacgacctgt
      781 tggagctggc ggctaagatc accgaccggc cgccggcccg cgagatggac atgctcctct
      841 cgaccggcga gcaggtgagc gtggccctga tggcgatcgc gattcactcg ctgggccaca
      901 aggcggtcag cctgaccgga gcccagatcg gcgtgaagac cgacgccagc tatcgcaagg
      961 cccggatcaa gtcgatcgag accacgcggc tgcggaaatc gctggacgag ggccacatcg
     1021 tcatcgccgc cggcttccag gggatcgacg acgacttcaa catcacgacg ctcggccgcg
     1081 gcggcagcga tacgacggcg gtcgccctgg cggcggtgct gcgggccgac gcctgcgaga
     1141 tttacaccga cgtcgatggc gtctatacga ccgacccgcg ggtcgtgccc gaggcccgcc
     1201 gcgtcggccg gatcggctac gacgagatgc tcgaactggc cagcctgggc gcgggcgtga
     1261 tgcacagccg cagcatcgag ttcggcaaga agttcgatgt gccgatccac gtgcggtcga
     1321 gctttacgga tcagccgggg acgatgatcg tcgcggagcc ggagcggccg gatttgcccg
     1381 taagcggggc agcgatcacc aaggacgagg ccctgatcac ggtgcagggc gttcccgacc
     1441 ggccggggac gagcctggag atcttcgcgc gggtcgcggc caggaatatc tcggtcgata
     1501 tgatcgtgca gaacgtgggc gagtcgggaa aagcggacat ctcgttcacg gtgccgcgga
     1561 acgagctcga tgtggccctg gaagcggtcc gcgaagcggt caagctggtc ggcgcggacg
     1621 cgataaccag cgacgacagc gtggccaagg tctcggtcgt cggcctgggg atggcgaagc
     1681 agacgggcgt cgcccagaag atgttccgcg ccctggccgc tgcgggcatc aacatccaga
     1741 tgatcacgac cagcgagatc aaaatctcgg tgctcgtcag ccgcgagcga tcgcaggagg
     1801 cgctgcggat cgtgcatcac gcgttcggac tggagagcgc ggcgcccgac gtagcccgcg
     1861 attccatcgc ggctgcccgc cacggtgtgg cgggctacgg caacggcacc gacgccgcgg
     1921 cggtggtcgc ccggctgcag agcatgaaca tggaggagct gacgatcgac ggcgtcgatc
     1981 tggacagctc gcaggcccgg gtaacgatca gcggcgtgcc cgacaagccg ggcatcgcgg
     2041 ccaaggtgtt cgaggacgtg gccgccgccg gcgtattcgt cgatatgatc gtgcagagcc
     2101 atcccgcagc tgccggcgcg gcgattttga gcttcaccgt gccgcagaat caactgacca
     2161 agagcgtcga ggccgcccgc aggatcgccc agtcgctggg ctgccagatg gtgtcgtcga
     2221 gcccgatcat cgccaagctg tcggtgagcg gcgtcggcct gcggacgcac gtcggcgtgg
     2281 ccatccgcat gttcaaggcg ctggccgagg ccggcatcaa cctggagatg atcaacacca
     2341 gcgaagtgag ggtgaacgtc gtcgtggacg gcgcagctgg cgagaagggc ctcaagcagc
     2401 tgcaggcggc gtttgcggat gtgctgcggt aggagtgagg tcagtcgtca gttgtcagtt
     2461 gtcagtggga ggcaactgac aaccagccgt tccaatcgtg cggccgttaa tgcctttagc
     2521 gacggcattt accaatcgga cttcacccgc aggccaccgc gtttgaccgc gccaaacccg
     2581 tggcctacgt ttcggtgggt cgcatgaagc cccgccgcgc gacccgtgct tcggcccgtt
     2641 ttttcgttac aggtgttgcg atgtcaccca ctttggccac ggttgtggag ccattcgtcg
     2701 atcggcgcaa ttacgcgggg caagccggtg ctcccacccg ggagcggcgg cagttcacca
     2761 acagccacga ggatctctcg cccgacgccc gcgagctggc ccaggcgatc gactcgtaca
     2821 agctggtcca ccgccggcgg ttcatcacct acgaagagat gctctcggtg ttcaagtcgc
     2881 tggggtacaa gcgcgactag ttatcgaggt tcccgctcgc ggcggctttt tctagctgcg
     2941 ggtggtcgct ttggacctcg gtaagcaggt tgtttgaaaa gagcacccgc ttggaagcat
     3001 cgccctccag cgtcagggcc ttggtggata ggcccgagaa cgtgttgccg ctgattgcga
     3061 tgtctgaagt gccggccagg acgatgcccg cggccgagcg gtcggcggtc gtgcgtttgt
     3121 ccttgccgtc accgatgtag ctgctcgaga agttgttgcc cgtgacggtg atccgcccgc
     3181 tggccgggcc gatccgcagg gcgtcctgct tgacgatagt gaacgtgttg ccgctcaccg
     3241 cgcagccgtg ggcgtcgcgc aggtcgatgc cgccgccggc ctcgtgggcg atcacgttgc
     3301 tggagagcgt gacgccgtag cagtcgcgat cgagaatgat cgccgtgcca ttgcattcct
     3361 cgatcatgtt gccgctgacg acgctgccgt aggtgttttc gatcacgacg ccgtgccgca
     3421 ggtggtcgtc gaggttattg cccgtcatgc agaggttgaa gccgtcgaaa caatgcaccg
     3481 cgtcctgatt ctcctcgaac tggttgccga ccacgacgat gtcgtggcag acgaccaggt
     3541 gcagcccggc ccctttgttg taggtcagca ggcagtcgca gacgcgcgga tcttcgtagc
     3601 actggtcgag gtaaatgccg tggccgccgt ggtagctgct ggtcacgccg tgcaggaaga
     3661 tctcatcgac atggtgggcc aggatgccgt cgccgctttt ctcgttgccg gtgacgcgga
     3721 ggttctgaat ctcgacccgc cacagcgtgg cccgcggatt ctccttccgc ttgggcgaat
     3781 cgatttcgat cgccggccgc ccgctcgtgt tcacattctt gatgtgcgtc gccgtgccgc
     3841 tgccggcgat gagcacgtcc tcctgcgaaa tccgcagcgg ctcggtgatt tcgaacgtcc
     3901 cggccggcag cctcacgacg ccgccggttg gcggcagggc gtcgatcgcc gcttgcaggc
     3961 tcggatagtc gatcgcctcg atggccggcc gcgcgccggg aagtgttgcc ttctgggcag
     4021 gcgattggct tccggcgggc tgccaggcca cgagcgcgct cgcagccagc agcagcgcgg
     4081 caagaacgaa ggtcaagcgc ttcatcgggc agttctcctt gaatcaagag tttgagcggc
     4141 gacgtcaggc gtcgcgctcg taggcttgct tcagccagcg gcggacttgc aggtcgatgt
     4201 cgtccagcga ggtcagcgcg acgcggtgcg tgatgcggtc ctttttggcg cggccgccgg
     4261 tgtcgagcag acgactcgtg aacggctctt cggcaagggc caagcccaag tcgacgcgct
     4321 tattggtcgt cggcttgatc tgggcaaaga cgtgccgccg gtagagcggc accattgtct
     4381 cgcacggaca aatccggacg ccctcgagct cgcgcgccag ttcgatcaga cggtcgtgga
     4441 ttggtcgcag gtgttctttc gggccagagt acatccgctc aacaaagacg ggagcgagct
     4501 tgagatagcc ggcgggcgtg tcttcggcca gcttgtcggg atggaacgcc ttctcggcca
     4561 gccaccacgc ggtgtttgcg ccaagccggt gctcgctcat cagccattcg cggctggctt
     4621 tctcgtcgcg cggcccggcc ttgcggatgt gcgccaacca atcgtcgagc gaccggccgg
     4681 tcttggcggg caattcgtcg atccacttct ggaccatcgc gatgcccgga tgcacggcat
     4741 agatcgccgc cgccgaagcg gctgcgcggt tacggacaga ctttgccttt cgcggccggc
     4801 gagaggcaag cagcgtggat ttggacatgg acttcgttgc gaaggaagtg cgggaccccg
     4861 caggtcgcca tgagtttcgc acgctggccg ggcaaactca accaacgcaa tcccgccgat
     4921 ccccaaaaag tataaacgcc tattgatttg tagtcggccg gacgctgata atcgcgaaac
     4981 tggctcgcca gaccgtaacg tgccctgcac cagcggtcat agatgagcgg ccggcggagt
     5041 gccggcaaac atcccacctg tggaggatca cccatgctat cgatcgactt gggggcgaaa
     5101 tcgcggtatt gcgacggcat ctcgcggcgg agcctgctga agctcggggc cctgggaatc
     5161 ggcgggctga cactgccggg gctcttgcgg gctgaagcag cggcgggcgt gcgttcgtcg
     5221 aacaaggcga tcatcaacat tcacctgagc ggcgggccga gccaccagga catgttcgac
     5281 ctcaagcccg aggctccgcg cgaattccgg ggcgaattca atccgatcaa gaccaacgtg
     5341 cccgggatgg aaatctgcga acacatgccg ctgctcgcga agatggccga caagtttgca
     5401 gtcatccgct cgctcgtcgg catgttcgac gaccacagca acttccacac gcagaccggc
     5461 ttctatcgcg acaacctgcg aaatgtcggc ggccgcccga gcatcggcag cgtcgtcagc
     5521 aagctgctgg ggccgaccgg caccggggct ccctgctaca tcgcctacaa cgacggcccc
     5581 gaggggtatc ttggtccagt tcataagcca taccggccgc agggcggcga cctgaaactc
     5641 gtcggcggca tggacgagac gcggctgagc acgcggacca acctgctcac ctcgctcgac
     5701 aggattcgcc gcgacaccga cggcagcggc aagatgaccg cgctcgattc gttcacacaa
     5761 cgggccgtcg gcgtggtcac cagcggcaag gtagccgacg cgctggacct ggagaaggaa
     5821 gacaaggccg ttcgcgagcg gtacggccgc gacgacggca gccagttcct gcgggcccgc
     5881 cgcctgatcg aggccggcgt ccgcgtcgtg acgttcgatt ggggcggctg ggatacgcac
     5941 ggcgacaact tcaatcagct gcggcggcag ttgccgaagc tcgaccaggg catgagcgcc
     6001 ctgctcaccg acctgtccga gcgcgggctt gataaggacg tgaccgtcgt aatgtggggc
     6061 gaatttggcc gcacgccgcg cgtcaacggc ggcgcgggcc gcgaccactg gtcgcgggtg
     6121 gcgatgtgct ttctcgccgg cggcggcatg cggctgggcc aggcgatcgg cacgtcgacg
     6181 gcctacgccg aagaagccaa ggaccgcccg gtgcagctgc aagaagtctt tgccacgctg
     6241 tatcacaact tgggcatcga cgtgaggcgt cagcagctca ttgatcccgc gggccggccg
     6301 cagtacctgg tcgaagttcg cgagccgatc aaggaattga tctagccggc gacgcagcga
     6361 gcgtcacgcc aattcgtttt ctcgcagcca cttcaagtag gtggctgcgc tcgcgtcgag
     6421 attctcgagc gagccgccgc cgcggatggg cgagcagatt tcgtaagtcg ccaggccgtc
     6481 aaagccgccg tcgcgaaggc cgcggaagaa ggcgcggtag tcgatgaatc ccgtcccgaa
     6541 cggcacggcc cgcgcccagt cgggctgcgt gcgctcgtag ttgaccagct ccggccggta
     6601 gcgatggcga ggcacgcgaa tgtagtcggc gttggtcgtg atggccgtat gcggcgcggc
     6661 taacttagcc gcggcataca tttcctcgcc gcgcagcgcg ggcgaccagg cgtcaaatcc
     6721 aagcttgcaa ttgggccgcc cgacgtcagc gagcaactcc agcagcgcgg cggtgtcgag
     6781 ggccaggtca tgatggttct gcaccgcgat cgtcacgccg tgctgcgcgg cgcggtcgca
     6841 catttcctgc agcgcgctgg cgacgcgccg ccagagcgtc tggggatcgt ggccgcccga
     6901 ttcgtatgcc gtgaagacgc ggacgacctt cgctcccagc tccgccgcga tgcgggccag
     6961 cgattcgacg taggcgatct gcatctccgc gtccggcacc tccgcggcgc cgggcgcgag
     7021 attcgtgtac gccgcaagca cttcgcacgt cacgcctgag cggctgagcg tggcctgcac
     7081 ctcgcgcagc agctgcggcg tcgcatccag cggcgacaga tgcggccgct tgcccgcgat
     7141 catcaccgac gagaacccca gccgcccggc gcgggcgatg aactccgtca gcggcaaggc
     7201 cgcctggccc cagaaaccgg cgtagctgac ggagaacaaa caggtcttca tttttcgtcg
     7261 agtgcatgct tcagccagta ttccaaaaac gccagctcga tctcgagggc ctccggggtc
     7321 ggcacgtgcg tcttgcgggc ggtcatcgcg acccgctgct tgtgcgcgag cacctcattg
     7381 acggcgatca ggtggttcag cgcccgccaa ttccgcggcg ggtcttccgt gccgccggaa
     7441 tcgagcaccg gacgcggcgc catcagcgcg tgcaggctca ccaggtcctc gcccgcttcg
     7501 cgcagctcct tatagagccc cgtccgcgga ttctcgtccg acggaattcc tggcttgcgc
     7561 tgcacgttgg gatcgaagcc cagataccag ggctcccagt aattcacgtt cgagttcttc
     7621 tcgtcgaaga cgatgcccgg atcggaccag acggcgcagg cgaacttctc gtgcaggcac
     7681 gacgcaaaca tcgaccactt gccgccgtac gacaggccga tgatcccgat tcgctcgggg
     7741 atcacctccg gcatctgcgc cagcgccgtg tggcagttgg ccgcgacata agccagcagc
     7801 gtcagcggct gccggcgctg ttccttgccc gcctcgacga gcagctgccg cgtctcgagg
     7861 ccgattttct cgaccgagcc cggcgtgccg atcgagagcg tcacgaagcc gcgcttcacg
     7921 agctgcaggc cgtagtcgtg cgtgccctgc cccttctggc ccgcaccgat gctcgtcagc
     7981 ggttcgtaga atggaaccag caccgccggg aacggccggt ttcccttggg aatcagcagg
     8041 tacccgtcgg ctaccttgcc gtccggcgaa acctgcacgt gcacgtgatg ctgcgtgtag
     8101 ccgtctttct ccacggactc cagccgcttc accgccggct tctccaccag aggtggccac
     8161 gctcccagcc gctcgtgcca gagcttgacg atttcgctcc gccgccgggc ccagtcggct
     8221 ggcgactgca cgcggctgcc atcggcaaat accagcggcg agcggtagtc gccaaattgg
     8281 ctagtgaact ccgccggcgg ctgcgtgaag ggcaccagct ttttcctgag ctccgcggcc
     8341 cgcggttctt ctgccgcagc gatcgctggc aacagactca aagtcaaaat gctgaagcgc
     8401 acgaggggca tgggagcgac tccggcggca agcggctgca acaacaggcc ttagcatagg
     8461 cggctgctgc cggcttacaa tcgaccggcc aaggaaacac tcgatgaaca ggctcaatcg
     8521 ccgcgaactg ctcggtttga ccttggcctc gtcggctggt ctggtggccg cgcagcaggc
     8581 ggcccccgct gtggatctgc accgccagct gcttgaactg gcggcacggc aagagcgcca
     8641 gcgccgcgag cgctttgccg cggtgaagac ggccgccgag ttggacgcac tgcaaaagaa
     8701 gctgcgggct gactttctag atttgctcgg aggccttccc tcgaacgacc gccctccctc
     8761 cgctcatacc actcgggcca tcgaggccga cggctatcgc atcgagaagc tcgttttcga
     8821 gagcttcccg ggctactttg tgccggcctt gctctatcgc ccgacaagtg caggcgggcc
     8881 gcggccggcg gtaatcagcc cctgtggaca ctccgccgtc ggcaaatcag ccccgccgta
     8941 tcagatgctg cacgtgaatc tggcgatgcg gggcttcatc gtgctgacct acgaccccgt
     9001 gggccagggc gagcgtagcc agttctggga cgcggccaag ggcgcatcgc ggttcaacct
     9061 ggtctgcggc gagcacgccg tgctgggcaa cccgctgtac ttactgggaa ccagcctcgc
     9121 tcgctaccgg atttgggacg gcatccgggc gatcgactat ctggcctcgc tgagcgacgt
     9181 cgatccaaaa cggatcggct gcgtcggcaa ttcgggcggc ggaacgctaa ccgcttacat
     9241 cgcggcgctg gattcgcgcg tggccgcagc cgcgatctgc tgctacatca cgacgctccc
     9301 gcggcgaatg ggtaaccgca ttcagcaaga cccggacgcc gatcccgagc aggacatttt
     9361 tggcttcgtc agcgccggca tcgaccacgc cgggctcttg gccctgcgag cgccgcggcc
     9421 caccctcgtc ggcgccgccc tggaggactt tttcccgatc gaaggagcgc gcgagtcgtt
     9481 cgccgaagcg cggcgactgt ttgaagtggc cggcactgcg gatcgcatcg ccctggccga
     9541 agcgccgggc cggcacgggc tgtccctgac gctgcgaaat gcggtgctac gctggttcga
     9601 ccgctggctc gccgggggaa aaggggatga gtcgatcgtc gaggcacccg tcgagccgcg
     9661 gccggcggcc gacctgcagg tgaccgaatc cgggcaggtc aacgtctcgc tccaatcgcg
     9721 gccgctgctg ccgttggcct gggagcagtt ccgcgctcag ccaatagcgc agcggctgcc
     9781 gctgcgcgaa atcgtgcagc ccgacgtcga ttcagccagc ttccgcctcg cggaactgag
     9841 cgctgcaaag cccggcaatt cgactgtggt cgtgctcatc aacggcaacg agtcgcccga
     9901 ctggcgggaa cagaaggagc tactgacggc cctcggcggg aggggccacg cggttgtctc
     9961 gctcgatccg cgcggcgtcg gagcgctgcg gccgaaggag ctgcgcgtcg gtggccgcga
    10021 ctacgccgac ccgttagccg gcgtcgagga aaacctggcc tacaatgcct ttctggtggg
    10081 caagtcgctg ctcggaatac gagtgagcga cgttctggcc gcagtccgtg aattgtcggc
    10141 caaatcgccg cggatagtgc tttgcggccg gcgcgactcc gccctcgtgg cctgcttcgc
    10201 ggcagcgctc gagccaaagg ttcacggcgt cgccgtccag gaaatgccgc tgagttttct
    10261 gccgctattt tccacggagg gaacgccgat caacgccgcg agcatcttgc ccggcgtcct
    10321 gcggtcgttc ggcgacttgc cggacgtctt ggctcagatc gccccgcgca agctgctggt
    10381 cgcgggggcc actggccaac gtccgcaagg actgagcggc gcttcgtttg ccagcgagcc
    10441 cttcacgaag aattcgcaat tgcttctcga ctggttggcc gccggctgat tcattctgga
    10501 aataggcggg caggcgggat acgttgaaag ggctgcgatt gccctcccgc cataaccccg
    10561 ccggaaaacg gagtgcccca tgctgcgact gctgctgact tcctcgctgg cgatgatcgt
    10621 ggccggcgca ggattgccgc caagcggtcg cggcggcgac tggccgcaat ggcgctatga
    10681 cgcgcagcgg agtgcggctt cgccggatga attgccggct cagttgcgta cccattgggt
    10741 ccgcgagctg ccgcggctga agccggcgtg gcctgatcag gccaagatgc aattcgacgc
    10801 ggcgtacgag ccagtcgcag ccggccagcg cttgttcgtc ggctcctcct tcgacaacac
    10861 cgtcaccgcc tacgacacgc ggagcggccg cgaactctgg acgttcttcg ccgacggccc
    10921 agtccggttc gcgccgctgg tctgggaaga caagctctat ttcgcctgcg acgacggcta
    10981 tctctactgc ctgaaggccg ccgacggctc gttaatctgg cggtttcgcg gcggaccctc
    11041 ggaccgcaaa atcctgggca acgagcggct gatctcgacc tggcccgccc gcggcgcgcc
    11101 ggtcgtcgct gacggcaagg tctatttcgc cgccggcatt tggcccttca tgggcatctt
    11161 cctgcacgcg ctcgacgccc ggaccggcga ggtgatttgg accaacgacg gcgacggctc
    11221 gacctatatg aagcagccgc acaacgccga ttcgttcgcg ggcgtcgcgc cacagggacc
    11281 gctggccgtc gttggcgagc ggctgttggt tccgggcggc cgctcggtgc cggcgtgcta
    11341 cgaccgggcg acgggcaaac tgatctacta tcaactggcg gaaaacgcca agaagggcgg
    11401 cggctcggct gtcgcggcca ccgatcaact gctgttcaac ggccgcgcgg tgttcgacct
    11461 ggcgacagag aaatacctcg gctccgcggg cgagttcgtc gcgttcgcgg gcggccggct
    11521 gtacgacggc gacgagggca agctccgcga aatcgacttg caggcgtcgg gcctttcgca
    11581 agagaagtcg atcgaccgca cgggcaagag cgccaccgct gccaaatggt cgatcaagca
    11641 gttgggccag gcgggtacgc cgaaggtcac tgcgctgatc aaggccgggt cgcggctgta
    11701 tgttggcagc tccggccgcg tgctggcttt tgctttgccg ctggacaccg aaggcgatgc
    11761 gaagcccatt tgggaggccg aattggaagg cacgccggtc agcctggtag cggccgacga
    11821 ccgtctgttc gccaccacgc ttgaagggcg gctctattgc ttcggcgttg cggatagggc
    11881 tccttc
//
LOCUS       DASRJB010000039        20217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_577615, whole genome shotgun sequence.
ACCESSION   DASRJB010000039 DASRJB010000000
VERSION     DASRJB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577615"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFB80_01050"
     CDS             complement(<1..571)
                     /locus_tag="VFB80_01050"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN32365.1"
                     /translation="MASRLNPPLALLALLLAPPLYVAAQGPDAVPPPAGPPLVPLQRV
                     ELDARASAAAAVVPDAPLIHTAQLVPAAFRDGAAAQLDQLLLQLQALLRSRGSDLPHV
                     AKLNLYVAREELGGEVLRAAASRFQGEQQPAVSLVATALPHREALVALDAVALAPARS
                     APAVRRGADAAILPAGSRIYVSGQAEPGTL"
     gene            complement(805..1632)
                     /locus_tag="VFB80_01055"
     CDS             complement(805..1632)
                     /locus_tag="VFB80_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN32366.1"
                     /translation="MSLETKDGVTLKATYYGGFNKKESVPVILIHGWEGTRGEYHTLA
                     LGLQAKGNAVIAPDLRGHGQSLVQKHADGTTKELDPEKFRQAELDAMVLDIEACKKFL
                     MEKNNQGELNINALCVVGADFGCILALRWSALDLTAPPLLAFKQGQDVKAIVLLTPMQ
                     TFKGLNYREALSVPALRSELSMMIAAGKQDSKGSAEAKRLYNSLHNLHAKVNEDDPEE
                     VKKKKDLFLVQPETSLTGTKLLGSGLPVALNVRNFIEFRLVNKMAEFPWTDRKRGAD"
     gene            complement(2022..2681)
                     /locus_tag="VFB80_01060"
     CDS             complement(2022..2681)
                     /locus_tag="VFB80_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32367.1"
                     /translation="MPAARSFRPLALVLFAAAACASAPGCTVFANMAYWMKGQVVPAK
                     FDGLESKRVAVVCLDANSLGGPGGEADAVARAVNVMLARNVPKITVVRQSEIADWIDG
                     QNQEVTDYTDIGRGVKADIVVGIDLISFNIHEGQTLLRGKANVHVRVYDMKRGGVIVH
                     EEQPREIAWPENGARHVTENEANFRANFIQELSFKIARDFYDYEAVQDYSIDANHLGA
                     N"
     gene            complement(2736..3206)
                     /locus_tag="VFB80_01065"
     CDS             complement(2736..3206)
                     /locus_tag="VFB80_01065"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HZN32368.1"
                     /translation="MSYSPPPAEPAALPPRAWCLHWVQRVAMVLAGGVLVALLVTASR
                     LTPSPRWMGTHQQLGLPPCTMVQWFGLRCPSCGMTTSWAHLTRGHLLASLRANSGGTL
                     LGIASVVCGPWLLVSGLLGRWIVRPPSEWVTLAAGLAIIGVTLIDWAIRLTFLR"
     gene            3434..3751
                     /locus_tag="VFB80_01070"
     CDS             3434..3751
                     /locus_tag="VFB80_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32369.1"
                     /translation="MRAKLLTLAALAAMLVGGMLMPSSADARPWRGYYGYGPYYGSYY
                     YGRPAVGYYGAYPAYGYGYYPYRSYNYGYYSYPYRTYYGPGYYNPGGGVYIGARPGVY
                     LRF"
     gene            complement(3833..4663)
                     /locus_tag="VFB80_01075"
     CDS             complement(3833..4663)
                     /locus_tag="VFB80_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZN32370.1"
                     /translation="MRLLLIGDIVGKPGERIVTRAVAGLRKREGLDLIVANAENAADG
                     SGLSPSIYRRLIAAGVDAITLGDHIYRRSEIASILSSADNIVKPANFPAAAPGKTWCV
                     VKAADGTPCAVISLLGRVFMRPVDCPWQAIDRVLAELPADVKVRLVDFHGEATSDKQV
                     MGRYLDGRVSAVLGTHTHVTTADEQLFPGGTAFQCDVGMTGPHESILGRRIDRVSETT
                     ITFQPTQFDVAEGDVRLCGTIVDCDPSSGRATAIRRLVVTAAEADQLAATGPSSPPLM
                     "
     gene            complement(4739..6301)
                     /gene="rny"
                     /locus_tag="VFB80_01080"
     CDS             complement(4739..6301)
                     /gene="rny"
                     /locus_tag="VFB80_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZN32371.1"
                     /translation="MFSGSEIIVLIVAILVAAGLTFAIVKFLDRLRKVDAENAAKQII
                     QKADQEASSKLKEADLAIKERELAQKAASDRELSKARDDIRERERTLDKRQEGLSQQE
                     DDLRKQTKMVETNQRRLSERMEDANRRHEELGKLLEMQRQTLHSISGLSPGEASKRLL
                     DSLEQELSQEMGSIILRHEKRAAEMVEQKSRDMLLTAMQRYAAAHTAESTTSTVDIPS
                     DEMKGRIIGREGRNIRTFEKVTGVDVIIDDTPGVVIVSGFDPVRREVARQTLNKLIAD
                     GRIHPSRIEEIFTATNKEIEAFIQKKGEEAAQEVNTHGVNPKVLYMLGRLHFRTSYSQ
                     NVLRHSVEVSFLSGMLAEMIGLDGDIARRAGLLHDIGKAADHELEGGHPKIGADLLKR
                     HGEKTEIVHAAFGHHDEIIAEYPYTMLVATADACSASRPGARRESLERYIKRMEELES
                     IACGFPGVEQAFAIQAGRELRVIASSKDTDDFKAAKICRDIAKAFEEQLTYPGEIKVT
                     VVRESRFQEVAR"
     gene            complement(6414..6647)
                     /locus_tag="VFB80_01085"
     CDS             complement(6414..6647)
                     /locus_tag="VFB80_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32372.1"
                     /translation="MLLFLLRLLLNLLVPSGGDRLSLAARADGRVEQVPAEIATRDRW
                     TSRKRAAAARRATSAPPPRASGEAPPLTDPNPS"
     gene            6914..7891
                     /gene="tilS"
                     /locus_tag="VFB80_01090"
     CDS             6914..7891
                     /gene="tilS"
                     /locus_tag="VFB80_01090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZN32373.1"
                     /translation="MPRLPEALAAAWPPAKWQDVTVLVAASGGADSVALLRGLVEIRD
                     AGEGRLIAAHFNHRLRGAESDGDQAFVEQLGRELGVKVVTGQREGEGVAEEALREARY
                     EFLNQMSGVYGARYVATAHTADDQVETVLLNVLRGTGLAGLAGIPRIRQLAESTTLIR
                     PLLEVTRGQVLEYLAALGQPFREDASNRSLQYTRNRLRHELLPLLERDYNPQVREALR
                     RLSQLAGEADDELSGRAARYRDFMPRTVSGGVELMTAPLELLSDYMVRTLFQVIWGEQ
                     GWPLQDMTLERWQQLAQFARATAPPPQMFPGGIRAEKQGDVMRLTRNPT"
     gene            complement(7899..9677)
                     /locus_tag="VFB80_01095"
     CDS             complement(7899..9677)
                     /locus_tag="VFB80_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32374.1"
                     /translation="MIYIASNPEYIPLFSVVCGTLLSGVARAMKDSASSAPLAANAHR
                     LLWGGFMAILAAGVGFSIRGGFLDNWRLEFGFTDTDVGNINGMGFNGFCFGIIIGGII
                     CDKIGYGKLVIAAFVMHLLSAVITFLPDASWTKGNASTCLTLGSFIFALANGTLEAVA
                     NPLVATLFFRNRTHYLNILHASWPAGLVLGSASGWVLDDVYKLHWKYQFALFLIPTVL
                     YGIMFLGQHFPKSEASQKGLSLGQMFKDVGMLGGGVACFLIGQFFFTLVTPWLAMAVS
                     PDWAWKIGLVIGVSIGGALWLAIAMMTQFSLGSPLLFVLFLAHAMVGAVELGTDSWIQ
                     NITGNILTSGQGKGLFVFTSLLMFALRFCSEFIEKRIGLSPVGILLVCAVLACVGLNL
                     SSRIETFAFALVALTVYGIGKTFFWPTMLAVASDRFPRTGAIAISFMGGIGMMSAGLN
                     GSPGLGYAKDRFAGEALQKVDATAFEKYKAEKPSTFLPIFPPATGLDGKKLGAVQEKL
                     AKAREELAKAGKDPKAALGQLSTEEQTVLAASIAGDRKTLVADSFIPAAMAVLYLGML
                     AYFASIGGYKPVHIDSTGTELGTAES"
     gene            9834..11195
                     /locus_tag="VFB80_01100"
     CDS             9834..11195
                     /locus_tag="VFB80_01100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN32375.1"
                     /translation="MTGPRQLPFGRTLALILAVALVLRLAAGWWWQQRLPPGTQFGFG
                     DSAGYWELAGHIARGEPYEAGPDRLKIFRTPGYPVALAPLFLLRDNPPVLWARAEAAL
                     FATAAVGGVAWLGRLLFDQRTALLAAAIAAVYPEAIALGAFVLSEAAFTPLMVLHLGL
                     WVVAWQAGSTPKVAGCSLAAGIAGGLATLVRPSWLLFVPFALVVGITLSLRQRNATEG
                     RNATEGVPYSAWRRQLLIGGMMLAGLCLAMLPWWIRNYRVAGRFVPTSLQVGASLYDG
                     ISPQATGASHMEFVGRFATAQRAADAQPGADTRGTFEERLDRRFRDAALDFARRDPAR
                     VLQLAGIKLLRMWSPLPNAADFQSTTLRLILAVTYTPLIMLALVGAWRFARRDWPCFL
                     FVMPAVYFTLLHVVFVSSIRYRQPALLPLIVLAAAVIVQWRVQRSGFRVQANDKTASQ
                     PEP"
     gene            11209..14541
                     /locus_tag="VFB80_01105"
     CDS             11209..14541
                     /locus_tag="VFB80_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HZN32376.1"
                     /translation="MSAPPTNSQPGLVARSLTELKRLLVLAAVLGLVGVAGKYYCFDR
                     LDEEIRVRVERLLRQHYQGLAVSVKSARRVAGQGIEIRGVRIAEVGDGSACVLVEIDE
                     IFAVCDTQLPDFLTRTPQFTELRLRRLKLRAERKPSGVWNLAHLLPLPSNPGSTPPAA
                     TITDGALEIVDPSANPPCSWALRNLELTVQPERSSDSRIQLHIRGTLAGDHLDRVEID
                     GLLDPAGGNWDVRGAVDGLEFSPRLRAALPRELSDALSPLSSIRGRTYFGFHAQRAGR
                     PASGMRQLPDGAAPTSTQTQREAYASRSPDEAPIHFTVHGKISEGRIDDARLPEPLTD
                     VAARIRIDNHVVQIDELSARCGATQIELSATIRGYGRGPIDVSDLNVRQLQLERWPLA
                     SLPPQLLEAWRRFSPRGTVDVAGALRYDGRTWRPDVTVQCHDLSLEYDRFRYRVTDGT
                     GTIGVQPDRVKVQLQCIGGGQYVRCWADVANPGPEFTGSIDIHTEGPIPIDDKLLAAL
                     DPSLRKLVRSFQPRGSAAVTAHFQRSPGEAAMHRRVDIQLHDCFIKHDRFRYPIDRVS
                     GLLELIDDDWHFRRLAGQNDSAAIGGSGSYLAGPNGRELVLDFTASDVPLADELKLAL
                     PPGVQQLWANLRPRGNIDHLQVRLGYHSANQKWSIEVQGDKFPFPRNPEGPITLEPAW
                     FRYALDDLRGSFHYKDGQVELKDLHAVHGQAAVSAAGVCRVSPDGSCRLDLEQLSADR
                     VLVDQDLLAALPAGMSQSLGRFPLEGPLNVIGRLGLTVVPQPEIPPALDWNLSLVLAD
                     GRLATATPVESLHGGVRLRGRQGAEGLYCHGELQFDSAVVRGVQLTGVQGPFWFDGRR
                     LVFGSLADRSAEGRAPQKMTAKLLGGRLALDGELKLGDESQFQVQAKLDNADLAEVAR
                     QLAPQQRGVSGRVFGEASVSGTALGKHTWRGEGRVWLREANIYQLPLMVRLLSLLSIK
                     PPDRTAFTTSDIDFLIEGDDLTFTRIDFSGDAISLKGKGRINGQRQLDLKFYPIVGRD
                     EWQWPLIGPLVGQTGREFMLIEVTGTLDRTDLKRTVFPRFDERLQQLFPELVRGSPIE
                     PAMPVERAARQAFNPLQWLPRR"
     gene            14564..14890
                     /locus_tag="VFB80_01110"
     CDS             14564..14890
                     /locus_tag="VFB80_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32377.1"
                     /translation="MSIGSLGIVGGLAATPVSQRAAEADKTQQATTEQTRSADAAEQA
                     ELAAGIGQTSEDSEAADRDADGRRLWERPPQQKPPAESPPAGPLAAPVAKDPSGACGG
                     QLDLVG"
     gene            complement(14900..16453)
                     /gene="hemG"
                     /locus_tag="VFB80_01115"
     CDS             complement(14900..16453)
                     /gene="hemG"
                     /locus_tag="VFB80_01115"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZN32378.1"
                     /translation="MPAVAPSPPLYAAPSGLIRRVAVIGGGISGLAAAHRLTELSPQS
                     HVTLFEAGERLGGALQSVRRDGWLIERSADMFITREPEAVDLCRRIGLADELIGTNTQ
                     HRRSWVVRRGKLVPIPEGFTLMSPAKIWPILTTPLLSPLGKLRLAAEYLTPRRADTSD
                     ESLESFVCRRFGREAFERLIQPLIGGIYTADPAQLSMAATLPQFVAMERKHGSLIRGM
                     RAGVGSRESGVGSRELASGGRQPAEDAASGARYGLFVAPRLGMQQLVEAIAAKLPTGC
                     VRLSCLVVRIEPHRVADGPRSPCWRVSLGGGGAEEFDDLILAAPGAASARLLAGVDDE
                     LSALIGRIPHAGCSIAVLGIRKEQASHPLDGFGFVVPAIENRRIIAGSLASVKFAGRA
                     PDGKVLLRVFVGGALQPELGQLPEEEIRRLVLEELRELIGLSGEPEFFEVIRWQGMMP
                     QYHVGHLDLVSQIEARSAAIPHFALAGNAYRGVGIPLCIRSGEAAAARIVTGSGVRGR
                     ESTDHRPTD"
     gene            16607..19036
                     /locus_tag="VFB80_01120"
     CDS             16607..19036
                     /locus_tag="VFB80_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN32379.1"
                     /translation="MYRCLSLLATAALALAAANAAAAEVDFGREVQPVLAAKCVRCHG
                     PEKRENGLRLDAGRTILKGGDNGPAVVARKSAESLMILAVTGKTDLVSKMPPKGEPLT
                     EAELALIRRWIDEGATFSSEAVAATKSKHWSFQRLVRPSQPLLRQAAWPAGAIDAFLL
                     ARLEKEGLAPSPPAGRPTLIRRAYLDLIGLPPAPDAVDAFVNDERPDAYERVVDQLLA
                     SPHYGERWGRHWLDLARYADSNGYTRDFGRQIWKYREWVIAAINRNQPFDEFTIEQLA
                     GDMLPAATQEQLVATGFHRNTLFNEEGGTDPEQFRVEAVVDRVNTTGSVWLGLTVGCA
                     RCHTHKYDPLSQVEYYQLFALLNNCDEPTIEAPSSLQRERGELALRDEIRGQIKQLEL
                     RLEEERPDLEAAQRAWEATVTPQQRARLPGPVQVAYDMKFEKRDEKNRQLIEEYFRQA
                     DVAREQFPLLQEVFELREVEPKIPTTMILRERKEPRETFVHKRGDFLDRGPDVTGGVP
                     AVLPPLQSSGERATRLDFARWLVSAENPLTPRVVVNRDWLQFFGRGLVETEDDFGTQG
                     TPPTHPELLDWLAVEFSRGWDSKALHRRIVTSNVYRQSSAVRSDLSSRDPQNKLLARQ
                     ARLRLDAEIIRDVALSAAGLLTTQLGGASVFPPQPEGVFDFTQDPKPWLAAKGGDRYR
                     RGMYTHFWRSSPYPMLMAFDAPAANVTCTRRLRSNTPLQSLTLANDQAFYECAAALAE
                     RVCTATADPSSRANLAFRLCLSREPTAAEAELLGRLVERETTSTPSDPAAAWTRICRV
                     LLNLDETITRE"
     gene            19041..>20217
                     /locus_tag="VFB80_01125"
     CDS             19041..>20217
                     /locus_tag="VFB80_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32380.1"
                     /translation="MQNSALRIPNSALLSARRHFFQQCRVGLGAMALGSLVAGERSRA
                     AEASDPLAARPPHFAPRAKSVIFLFMAGGPSQLEMFEPKPDLQKLDGQVIPESFVKTK
                     RFAFIKQDAKLLGTKRTFAQYGESGQTISECLPHLAGVADDICTLRAVKTDVFNHGPA
                     KFFLNTGSPLFGRPSMGAWITYGIGSLSRDLPGFVVLQSGPRGPRGGAPNWGSGFLPT
                     TYQGVPFRSTGAPILNLASPEGTSPDDQRRFIESVNEFNRLRLADTGDGEIATRIAAY
                     EMAFRMQSSSPELMDLSGESAETLALYGAEAGKPSFAMNCLLARRLVERGVRFVQLYH
                     TDWDHHGNKGTELGESLDAICKQTDQPAAALVRDLKRRGLLDETLVVWGGEFGRTPQG
                     "
BASE COUNT         3360 a         6778 c         6713 g         3366 t
ORIGIN      
        1 ggagagtgcc gggctcggcc tggccggaaa cataaatccg gctgccggcg ggcaggatcg
       61 cggcgtcggc ccctcgcctg acggcgggcg cggacctggc cggtgcgagg gccacggcgt
      121 cgagggccac cagcgcctcg cgatgcggca gcgccgtggc caccaggctc acggcaggct
      181 gctgctcgcc ctggaagcga gacgccgccg cgcggagcac ttcgccgccc agctcttcgc
      241 gagcgacgta gagattcaat ttggcgacgt gcggcaggtc gctgccgcga cttcgcagca
      301 gcgcttgcag ctggagcagc agctggtcga gctgcgcggc cgcgccatcg cggaaagcgg
      361 cgggcaccag ctgcgcggta tgaatcagcg gcgcatcggg gacgaccgcc gccgcggcac
      421 tggcccgcgc gtccagctcc acgcgctgga ggggcactag cggcgggccc gcaggaggag
      481 gcacggcgtc cgggccttgc gctgccacgt agagcggggg agcgagcagc agcgccaaga
      541 gcgccagcgg cgggttcagt cggcttgcca cttcgttccc tttccgcagt taatcccgag
      601 gccgccgtga ccgtggttgc ccgccgcggc tggtggcact ggcgtctcgc cagtgcgcgg
      661 cgggccttct cggtaaccgt tgccctgcaa atcaacttgg cctggaagtt tggcctgttc
      721 gaaaatggcc attggaacgc gggtccaggc gcttgctgct gtcccttcgt tcggcactgg
      781 cggcacgcca gtgccacgcg cgggctagtc ggccccgcgc ttgcggtcgg tccaggggaa
      841 ctcggccatc ttgttaacca accgaaattc gatgaagttg cggacgttga gcgccacggg
      901 caggccgctg cccagcagct tggtgcccgt caggctcgtc tcgggctgca cgagaaacaa
      961 gtccttcttc ttcttgacct cctccggatc gtcctcgtta accttcgcgt ggaggttatg
     1021 cagcgagttg tacagccgct tggcttcggc cgagcctttg ctgtcctgct tgccggctgc
     1081 aatcatcatc gacagctcgc tccgcagagc cggtacggag agggcctcgc ggtagttgag
     1141 acccttgaag gtctgcatcg gagtgagcag aacgatggcc ttgacgtcct gcccctgctt
     1201 gaacgccaac agcggcggag cggtgaggtc gagggccgac cagcggagcg ccaggatgca
     1261 gccgaagtcg gcgccgacga cgcacagggc gttgatgttc aactcaccct ggttgttctt
     1321 ctccatcagg aactttttgc aggcttcgat gtcgaggacc atggcgtcca gctcggcctg
     1381 ccggaacttc tccgggtcca gttccttggt cgtgccgtcg gcgtgcttct ggaccaggct
     1441 ttggccgtgg ccgcggaggt ccggcgcaat gaccgcgttc cccttggctt gcaggcccag
     1501 cgccagcgtg tggtattcgc cgcgcgtccc ttcccagccg tggatcagga tgacgggcac
     1561 cgactctttc ttgttgaacc caccgtaata ggtcgccttg agagtgacgc cgtccttggt
     1621 ttcgagggac acgtcctcgg gttccggaat cttggggcct tttccgggcg gggccttcgc
     1681 agcggcagga ggcgcgcctt tggcggtagg aggcggcgcg cccttggcgg ccggaggggg
     1741 cgcgcccttc gcaccgggcg gaggcggggc cttggtcggc ggggcctgcg ccgcgagcca
     1801 atcggaagcc gcgataacca gaactaagac gctcaaaccg accccgcgcc gcccaatcat
     1861 gcctgcctgc ctcatggaac acccgtagag ttcggcccct ggtggaccgc taaaatgccg
     1921 caatccggcc gccctagctc atcgtagaag gaagggcagt ggccgtcaat taaatgccag
     1981 gggtgtgatg cgcgctcggc ctggccggcc tgcggccccg actagttcgc ccccaggtga
     2041 ttggcgtcga tcgagtagtc ctggacggcc tcgtagtcgt agaagtcgcg ggcgatcttg
     2101 aacgagagct cctggatgaa gttggcccgg aagttggcct cgttctcggt gacgtgccga
     2161 gcgccgtttt cgggccaggc gatttcccgc ggctgctctt cgtggacgat gacgccgccc
     2221 cgcttcatgt cgtacacgcg gacgtgcacg ttggccttgc cgcggagcag cgtctggccc
     2281 tcgtggatgt tgaacgaaat caggtcgatg cccaccacga tgtcggcctt cactccgcgg
     2341 ccgatgtcgg tgtagtccgt gacttcctgg ttctgcccgt cgatccagtc cgcgatttcc
     2401 gactgccgga cgaccgtgat cttcggcacg ttgcgggcca gcatgacgtt cacggcccgg
     2461 gccaccgcgt cggcctcgcc gcccggaccg ccgagggagt tggcgtcgag gcagaccacc
     2521 gcgacgcgct ttgactccag cccgtcgaac ttggcaggaa ccacctggcc cttcatccaa
     2581 taggccatgt tggcgaacac ggtgcagccc ggggcgctcg cgcacgcggc agccgcgaag
     2641 aggaccagcg ccagcgggcg gaaggagcgt gcagccggca tgaggcaatc ctttgctcaa
     2701 gggccgggct tcctgcccgg cagtttcccg cgatctcacc gcaaaaacgt cagccgaatc
     2761 gcccagtcga tcagcgtcac tccgatgatc gccaatccgg cggccagcgt gacccactcg
     2821 ctcggcggcc gaacgatcca ccgcccgagc agtcccgaca cgagcagcca gggcccgcaa
     2881 accaccgacg cgattcccag cagcgtcccg cccgagttcg cccgcagcga ggccagcagg
     2941 tggccccgcg tgaggtgcgc ccaggaggtc gtcatcccgc acgacgggca ccgcaggccg
     3001 aaccactgca ccatcgtgca cggcggcagg cccaactgct ggtgcgtgcc catccagcgc
     3061 gggctcggcg tcagccggct ggctgtgacg agcagcgcta cgagcacgcc gccggccagg
     3121 accatcgcca cgcgctgcac ccaatgcagg caccaggcgc gcggcggcag tgcggccggt
     3181 tcggcgggcg gcggagaata gctcacgagc gctggcctca caagagagaa atgcagtcta
     3241 acgcgagagc accgcgagcg gtcaggtgtc ccctgaattt tccgccatct gcggggcgca
     3301 agtcgtttga gaaccgcacc ttggggaatc gcgccctggc ctcgtcccgc cgcggcttta
     3361 ggcttgaagt agcggctggc gcggcccttc gcagccggct gcgaggcgta cacatttcgg
     3421 gaaggagaaa gtcatgagag ccaaattact gacacttgcg gcgctggccg cgatgctcgt
     3481 tggcggcatg ctgatgccga gttccgccga cgcgcggccg tggcgcggct attacgggta
     3541 cgggccatac tacgggagct actattacgg ccgaccggcg gtcggctact acggggcgta
     3601 cccggcgtat ggctacggat attacccgta tcgctcctac aactacggct actacagcta
     3661 cccgtaccgc acctactacg gaccggggta ctacaatccg ggcggcggcg tctacatcgg
     3721 cgcgcggccg ggcgtctacc tccggttcta ggtagggtgt gactaggccg ccgcgaccga
     3781 cggcacaccg aagcgaaggt gggccggcct cgctccgctc gctggtccca ccctacatca
     3841 agggcggcga gctcgggccg gtcgcggcga gttgatccgc ttccgccgcc gtcacgacga
     3901 ggcggcggat ggcggtggcg cggccgctgg agggatcgca atcgacgatg gtcccgcaca
     3961 ggcggacgtc cccctcggcg acgtcgaact gcgtgggctg gaacgtgatc gtggtttcgc
     4021 tgacgcggtc gatgcggcgg ccgaggatgc tttcgtgcgg gccggtcatg ccgacgtcgc
     4081 actgaaacgc cgtgccgccg ggaaagagct gctcgtcggc cgtggtgacg tgcgtgtggg
     4141 ttcccaacac ggcgctcacc cggccgtcga ggtagcggcc catcacctgc ttgtcgctgg
     4201 tggcttcgcc gtggaaatcg acgaggcgca ccttgacgtc agcaggaagt tcggccagca
     4261 cgcggtcgat ggcttgccag gggcaatcga cggggcgcat gaagacgcgc ccgagcaggc
     4321 tgatcacggc gcagggcgtg ccgtcggcgg ccttgacgac gcaccaggtt tttcccggtg
     4381 cggctgccgg gaagttggcc ggtttgacga tgttgtccgc gctgctcaag atgctggcga
     4441 tctcgctgcg gcggtagata tgatcgccca gcgtgatcgc atcgacgccg gcggcgatca
     4501 gcctgcgata gatgctgggc gacaggcccg agccgtcggc cgcgttctcg gcgttggcca
     4561 cgatgaggtc cagcccctca cgctttcgca agccggctac cgcgcgggtc acgatccgct
     4621 cgcccggctt gccgacgatg tcgccgatga ggagtaatcg catagtcggt tgtcagtgat
     4681 cggtgggtcg gtggtcggtt ggcctgcccc aacggactac tgacgacgga caactgacct
     4741 atctcgctac ttcctgaaac cgcgattcgc gcaccaccgt gaccttgatc tcgcccggat
     4801 acgtgagctg ttcctcgaac gccttggcaa tgtcgcggca gatcttggcg gctttgaagt
     4861 cgtcggtgtc cttgctgctg gcaatcaccc gcagctcgcg gccggcctgg atggcgaagg
     4921 cctgctcgac cccggggaag ccgcaggcga tcgactccag ctcctccatc cgcttgatgt
     4981 accgctccag cgactcgcgc cgcgccccag gccggctggc gctgcaggcg tcggccgtcg
     5041 cgaccagcat cgtgtaggga tattcggcga tgatctcgtc gtggtggcca aacgcggcgt
     5101 gcacgatctc ggtcttctcg ccgtgccgct tgagcaggtc cgcgccgatc ttcgggtggc
     5161 caccctcgag ctcgtggtcg gccgccttgc cgatgtcgtg caacaggccc gcccggcggg
     5221 cgatgtcgcc gtcgaggccg atcatctcgg ccagcatgcc gctgaggaag gagacctcga
     5281 cgctgtgccg cagcacgttc tggctgtagc tcgtgcggaa gtgcagcctg ccgagcatgt
     5341 acagcacctt gggattgacg ccgtgcgtat tgacttcctg ggccgcctcc tcgcccttct
     5401 tctggatgaa ggcctcgatc tccttgttcg tcgcggtaaa gatctcctcg atccgcgagg
     5461 ggtggatgcg gccgtcggcg atcagcttgt tgagcgtctg gcgggcgacc tcgcggcgga
     5521 cggggtcgaa gccgctgacg atcaccacgc cgggcgtgtc gtcaatgatc acatccacgc
     5581 cggtcacctt ttcgaaggtg cggatgtttc tcccttcgcg gccgatgatc cgccccttca
     5641 tctcgtcgct gggaatatcg acggtgctgg tcgtgctctc ggcggtgtgg gcggcggcgt
     5701 agcgctgcat cgccgtgagc agcatgtcgc gcgacttttg ctcgaccatt tccgctgccc
     5761 gcttctcgtg ccgcaggatg atcgagccca tctcctgcga cagctcctgc tcgagtgagt
     5821 ccagcagccg cttgctggcc tcgcccggcg agaggccgct gatcgaatgc agcgtctgcc
     5881 gctgcatctc gagcagcttg cccagctctt cgtgccggcg gttggcgtct tccatccgct
     5941 cggagagccg ccgctggttc gtctcgacca tcttggtctg cttgcggagg tcgtcctcct
     6001 gctggctgag gccctcctgc cgcttgtcga gcgtccgctc gcgttcgcgg atgtcgtcgc
     6061 gggccttcga cagctcgcgg tcgctggccg ccttctgggc cagctcgcgc tccttgatgg
     6121 ccaggtcggc ctccttcagt ttgctgctgg cctcctgatc ggccttctgg atgatctgct
     6181 tggcggcgtt ctcggcatcg actttccgca ggcggtccaa aaacttgacg atggcaaagg
     6241 tcagtcccgc cgcgaccagg atcgcgacga tcagcacgat gatttcagag ccagagaaca
     6301 cgccagccgc cttccgatgc aaagcgagcg gcgtctgtgg gcggcgaggc gctttcgagg
     6361 caggcctcgg ctagaagcaa cgtcgtgtcg gatggtgacc cgcgatcggc cggctatgac
     6421 gggttcggat cggtcaacgg aggcgcctcg ccgctggcgc gcgggggcgg cgcactggtg
     6481 gcgcgccggg cggctgcggc tcgcttacgg ctggtccacc ggtcgcgcgt ggcgatctcg
     6541 gcgggaactt gctcgacccg gccatcggcc cgggcggcca gcgacaagcg gtcgccaccg
     6601 gacgggacca gcagattgag caagagccgc aagagaaaga gaagcatcga agcactccac
     6661 tgtcagtcgt tcacgaaccc agttttccgg ccgaagtgcg cggcacatgc gccgcgcgcc
     6721 gacggccagc gaaatcaaca agcgacaaac gttggcctcc gcacggcgcg gccggccgcc
     6781 cacaaacaca atcgcgaata ggtgataatc aagtttgggc ggaagaccca tcgtccccgc
     6841 cgccagcgat atgttaccag agatggcggc cagttcaaca aggtaaacgg caatccgcgt
     6901 gccgaaggcc gccatgccca gactgcccga agcgctggcc gctgcctggc cccccgcgaa
     6961 atggcaagac gtgaccgtcc tggtcgccgc cagcggcggc gccgacagcg tggccctgct
     7021 gcgggggctg gtcgaaattc gcgacgccgg cgaaggccgg ctgattgccg ctcacttcaa
     7081 ccaccgcctc cgcggggccg agtcggacgg ggatcaggcg tttgtcgagc aattggggcg
     7141 ggagttgggg gtgaaggttg ttacgggtca gcgagaggga gagggcgtgg cggaagaagc
     7201 gctgcgcgag gctcggtacg agtttttgaa tcaaatgagc ggggtttacg gggcgcggta
     7261 tgtcgccacg gcacacacgg cggatgacca ggtcgagacg gtgctgctga acgtgcttcg
     7321 cggcaccggg ctggccggcc tggcggggat tccgcgaatt cgccagctgg ccgagtccac
     7381 gaccctcatc cgtccgctgc tcgaggtgac tcggggccag gtgctcgaat acctcgccgc
     7441 gctggggcag ccctttcgcg aggacgccag caaccgcagc ctccagtaca cccgcaaccg
     7501 cctccgccac gagctgctgc cgctattgga gcgcgactac aatccgcaag tccgcgaagc
     7561 actgcggcgg ctgagccagc tggcgggcga ggccgacgac gaattgagcg gccgagccgc
     7621 tcggtaccgg gacttcatgc ctcgcacagt atccggcggc gtcgaattga tgacagctcc
     7681 gctggagctt ctgtccgact atatggtccg cacgctcttc caggtcatct ggggggaaca
     7741 gggctggccc ttacaagaca tgacgctgga acggtggcag cagctcgctc aattcgcccg
     7801 cgccaccgca ccgccgcccc agatgttccc cggcggaatt cgcgcggaga agcagggcga
     7861 cgtgatgcgc ctgactcgaa atccgacctg actcaccgtc acgactccgc cgttcccagt
     7921 tctgtccctg tgctgtcgat gtgcaccggt ttgtagccgc cgatgctggc aaagtaggcg
     7981 agcattccta gatagagaac tgccatcgcc gccggaatga atgagtcggc caccagcgtc
     8041 tttcggtcgc ccgcgatgct ggccgcaagg accgtttgct cttcggtgga gagttggccc
     8101 agggcggctt tcgggtcctt gccggccttc gccaattctt cgcgggcctt ggccagtttt
     8161 tcctgcaccg cgccgagctt cttgccgtcc aggcccgtcg ccggcgggaa aatcggcaga
     8221 aacgtgctgg gcttttcggc cttgtacttc tcgaacgcag tcgcgtcgac tttttgcagc
     8281 gcttcgcccg cgaagcggtc cttggcatag cccagacctg gcgaaccatt gagtccggcc
     8341 gacatcatgc cgatgccgcc catgaacgag atcgcgatgg cgcctgtacg cggaaagcgg
     8401 tccgaggcca cggccagcat cgtcggccag aagaacgtct tgccgattcc atagaccgtg
     8461 agggccacca gcgcgaatgc aaaggtctcg atgcggctgc tcaaattgag ccccacgcag
     8521 gccaggaccg cgcagaccag gaggatgccg accggcgaga ggccgatgcg cttctcgatg
     8581 aactccgaac agaaccgcaa ggcaaacatc aagagcgaag tgaagacgaa cagccccttg
     8641 ccctgcccgg aagtcaggat gttgccggta atattctgaa tccagctgtc agtgccgagt
     8701 tcgaccgccc ccaccatcgc atgggccaaa aacaggacga acagcaatgg cgaaccgagc
     8761 gagaactgtg tcatcatcgc gatggccagc cacagcgctc caccgatact gacgccgatg
     8821 acgaggccaa tcttccaagc ccagtcgggc gacacggcca tggccaacca aggcgtgacc
     8881 aaggtgaaga agaattgacc gatcaaaaag cacgccacgc caccgcccag catgccgacg
     8941 tccttgaaca tctggcccag gctgagcccc ttctgcgatg cctccgattt cgggaaatgc
     9001 tggcccaaga acatgatgcc gtagagcacc gttggaatca gaaacaaggc aaattgatac
     9061 ttccaatgca gcttgtagac atcgtccagc acccaacccg aagcgctgcc cagcaccaac
     9121 cccgcaggcc agctggcgtg caaaatattg aggtagtggg tgcgattgcg gaagaacaac
     9181 gtggcgacga gcggattggc cacggcctcc agcgttccgt tggcgagggc aaagatgaac
     9241 gaccccagcg tcaagcacgt gctcgcattg cccttggtcc aactcgcatc gggaagaaaa
     9301 gtgatgaccg ccgacaggag gtgcatgacg aacgcggcga taaccagctt tccgtaaccg
     9361 attttgtcgc aaatgatccc gccgatgatg atgccgaagc agaagccatt gaagcccatg
     9421 ccgttaatgt tgccgacatc ggtgtcggtg aagccaaact ccagtctcca attgtcgaga
     9481 aacccgccgc ggatggaaaa gccgacgccc gcggcgagaa tcgccatgaa gccgccccag
     9541 agcaagcgat gggcattggc ggcaaggggg gcggaggacg ctgaatcttt catggccctg
     9601 gcaactccgg agagaagagt tccgcaaacg acggaaaaca gcggaatata ctcaggattg
     9661 ctagcaatgt aaatcaagac ttccgcagga atcttggacg atccgtcgca acaggacgcc
     9721 gcagcgcgtt caacgatccg ctagcggggc gctcgtcttg accgcgggcg ctagcccttc
     9781 gtaccatctg ccgccatttt ccctttttgc gtctttggcc cgcaaagcga ctcatgacag
     9841 ggcccaggca actgccgttt ggccgtacgc tggccctgat cctggccgtg gcgctggtgc
     9901 tgcgcttggc ggcgggatgg tggtggcagc agcggctgcc gccgggcacg cagttcggct
     9961 ttggcgatag cgcgggctac tgggaactgg ccggccacat cgcccgcggc gagccgtacg
    10021 aagccggtcc agaccggcta aaaatcttcc gcacgccggg ctatccggtg gccctcgcgc
    10081 cgctcttcct gctgcgcgac aatccgccgg tgctctgggc gcgggccgag gcggcgctct
    10141 tcgccacggc ggccgtcggc ggcgtcgcct ggctgggccg gctgctgttc gaccagcgga
    10201 cggcgctcct cgccgcggcc atcgcggcgg tttatcccga ggcgatcgcc ttaggggcct
    10261 ttgtcttgag cgaagccgcg ttcacgccgc tgatggtgct gcacctggga ctgtgggtcg
    10321 ttgcctggca ggcgggcagc acgcccaagg tcgctggatg ttcgctggcc gcggggattg
    10381 ccggcggcct ggcgacgctg gtgcggccga gctggttgct cttcgtaccg tttgcgctgg
    10441 tcgtcggaat aacgctttcc ttacggcaga ggaacgccac agagggccgg aacgccacag
    10501 aaggcgttcc ctacagcgcg tggcgcaggc agctactgat tggcggcatg atgctggctg
    10561 gcctctgcct ggcgatgctg ccgtggtgga ttcgcaatta ccgtgtggcg ggccgcttcg
    10621 ttcccacgtc gctccaggtc ggagcgagcc tgtacgacgg gatcagcccg caggccaccg
    10681 gcgcgagcca catggaattc gtgggccggt ttgcgaccgc ccagcgcgcg gccgatgccc
    10741 agcccggcgc ggacacgcgc ggcacgttcg aggagcgtct cgaccggcgg ttccgcgacg
    10801 ccgcgctcga cttcgcccgc cgcgatccgg cccgcgtcct gcagctcgcc ggcatcaagc
    10861 tgctccgcat gtggagcccg ctgcccaacg cggccgattt ccagagcacc accctccggc
    10921 tgatcctcgc cgtgacctac acgcccctga tcatgttggc gctggtcggc gcgtggcgat
    10981 tcgcccggcg cgactggccc tgcttcctct tcgtaatgcc cgccgtgtat ttcacgctgc
    11041 tgcacgtcgt gttcgtcagc tccatccgct atcgccagcc cgccctgctg ccgctgatcg
    11101 tcctggcggc ggccgtcatc gttcagtgga gggttcagcg ttcagggttc agggttcagg
    11161 cgaacgacaa gaccgcctca caacctgaac cctgaacccc gaaccccaat gtctgctccg
    11221 cccacgaaca gccagccggg cctcgtcgcc cgctcgctca ccgagctgaa acggctcctg
    11281 gtgctggcgg cggtcttggg actggtgggc gtggcgggca agtattattg cttcgaccgg
    11341 ctcgatgaag agatccgggt tcgcgtcgag cggctgcttc ggcagcacta ccaggggctg
    11401 gccgtctcgg tgaaatcggc ccgccgcgtg gccggacagg gaattgagat tcgcggcgtc
    11461 cgcattgccg aagttggcga cggctcagct tgtgtcctcg tcgaaatcga cgagatcttt
    11521 gccgtctgcg acacccagct gcccgatttt ctcacgcgca cgccgcaatt cactgagttg
    11581 cggctgagac ggctcaagct ccgcgccgag cgcaagccga gcggcgtctg gaacctcgcg
    11641 cacctgctgc cgctgccgtc aaacccgggc agcacgccgc cggccgccac gattaccgac
    11701 ggcgcactcg aaatcgtcga tccttcggcc aatccgccct gctcgtgggc gctgcgaaac
    11761 ctcgagctga cggtgcagcc cgagcgaagt agcgacagtc gaatccagct gcacatccgc
    11821 ggcacgctgg ccggcgacca tctggatcgc gtggagatcg acggcctgct ggatccggcc
    11881 ggcggcaact gggacgtccg cggcgccgtc gatgggctcg aattcagccc gcgcctgcgg
    11941 gccgcgctgc cgcgcgagct gagcgatgcg ctctctccgc tgtcgagcat ccgcgggcgg
    12001 acgtacttcg ggtttcacgc ccagcgtgcc gggcgtccgg cgagcgggat gcgtcagctt
    12061 cccgatggcg ctgcgccgac ctccacccag acccagcggg aggcttacgc ctcccgctcg
    12121 ccagatgagg cgccgattca cttcaccgtc cacggcaaga tttccgaggg ccggatcgac
    12181 gatgcccggc tgccggagcc gctgacggat gtcgccgcgc ggattcgcat cgacaaccac
    12241 gtcgtgcaga tcgacgagct ttcggcccgc tgcggagcga cacagatcga actctcggcg
    12301 acgattcgcg gctacggccg cgggccgatc gacgtgtcgg acctgaacgt ccgccagctg
    12361 cagctggagc gctggccgct ggcatcgctg ccgccgcagt tgctcgaggc gtggcgtcgc
    12421 ttttcgccgc ggggcaccgt cgatgtggcc ggggcgctgc gctatgacgg ccgcacctgg
    12481 cggcccgacg tcacggtgca atgccacgac ctgtcgctcg aatacgaccg cttccgctat
    12541 cgggtgaccg atgggaccgg caccatcggc gtgcaacccg accgcgtcaa ggtgcagctg
    12601 cagtgcatcg gcggcggtca gtacgtacgg tgctgggccg atgtggccaa tcccgggccg
    12661 gagttcacgg gttcgatcga catccacacg gaaggcccga tcccgatcga cgacaagctg
    12721 ctggcagccc tcgatccatc gctccggaag ctcgtccgct cattccagcc gcggggcagc
    12781 gccgccgtga ctgcccattt tcagcgctcg ccgggcgagg ctgcaatgca ccgccgagtg
    12841 gacatccagt tgcacgattg cttcatcaag cacgaccggt tccgctatcc gatcgaccgc
    12901 gtcagcggtc tcttggaact gatcgacgac gattggcact tccgccggct ggccgggcag
    12961 aacgacagcg cggcaatcgg cggcagcggc tcgtatctgg ccggacccaa cggccgggag
    13021 ctggtgctcg acttcaccgc ttcggatgtg ccgctggccg acgagctgaa gctggcgctg
    13081 ccgcccggcg tgcagcaact gtgggccaat ctgcggccgc gcggcaacat cgaccacctc
    13141 caggttcggc tcggatatca ctccgcgaac caaaagtggt cgatcgaagt ccagggggac
    13201 aagttcccct tcccacgcaa tcccgaaggg ccgatcacgc tggagcccgc ctggttccgc
    13261 tacgccctgg acgacctccg cggcagtttt cactacaagg acggccaggt ggagctcaag
    13321 gacctgcacg cagtgcacgg ccaggcggcc gtgagcgccg cgggcgtctg tcgcgtctcg
    13381 ccggacggga gctgccgcct cgacctcgag cagctcagcg ccgaccgcgt gctggtagat
    13441 caggatttgc tggcggcgct gccggccggc atgtcgcagt cgcttggccg ctttccgctg
    13501 gaaggccctc tgaacgtcat cggccggctg gggctgaccg tcgtgccgca gccggaaatt
    13561 ccacccgcgc tcgattggaa cctgtcgctg gtgctggccg acggccggct ggcgactgcg
    13621 acgccggtcg agagcctgca cggcggcgtg cggctgcggg gccgccaggg cgccgaaggt
    13681 ctgtattgcc acggcgagtt gcagttcgat tcggccgtcg tccgcggcgt gcagttgacc
    13741 ggcgtgcaag gcccgttctg gttcgacggg cggcggctgg tcttcggctc gctggccgac
    13801 cgcagtgccg agggccgcgc gccgcagaaa atgacagcga agctgctggg cggccgatta
    13861 gcgctcgacg gcgagctgaa actcggcgac gaaagtcagt tccaggtgca ggcgaagctc
    13921 gacaatgccg acctggcgga agtggcccgc cagctcgctc cgcagcagcg cggcgtcagc
    13981 ggccgcgtgt tcggggaggc cagcgtcagc ggcaccgccc ttggcaaaca cacctggcgc
    14041 ggtgaaggcc gggtctggct gcgcgaggcc aacatctacc agctgccgct gatggttcgc
    14101 ctgctcagct tgctcagtat caagccgccg gaccgcaccg cgttcaccac cagcgacatc
    14161 gacttcctga tcgaagggga tgacctgacc ttcacgcgaa tcgacttcag cggcgacgcg
    14221 atcagcctca aaggcaaggg ccggatcaac ggtcagcggc agctcgacct gaagttctac
    14281 cccatcgtcg gccgcgacga atggcagtgg ccgctgatcg gaccgctggt cggccagacc
    14341 ggccgcgagt tcatgctgat cgaagtcacg ggcacgctcg accgcaccga cctcaagcgg
    14401 acggtcttcc cgcggttcga cgagcggctc cagcagctgt tcccggagct ggtgcgaggc
    14461 agtccgatcg agccggcgat gccggtcgaa cgggcggcgc ggcaggcctt caatccgctc
    14521 caatggctgc cgcgaaggta gaattcgtca gaggacctct ggtatgagca tcggctccct
    14581 cggcattgtc ggcggcctgg cagcgacgcc cgtgtcgcag cgcgcggccg aagccgacaa
    14641 gactcagcag gcaacaaccg agcaaacgcg ctccgccgac gccgcggaac aagccgagct
    14701 ggcggccggc atcgggcaga cctcggagga ctccgaggcg gccgaccgcg acgctgacgg
    14761 ccgccggctc tgggagcggc cgcctcagca gaagccgccg gccgaatcgc cgccggccgg
    14821 tccgctcgcc gcgcctgtgg cgaaggatcc ctccggcgcc tgcggcggcc agctcgacct
    14881 cgtcggatag acagttcggt cagtcagtcg gtcggtggtc ggttgactcc cgacccctga
    14941 ctcccgatcc tgtcacaatc cgcgccgccg ccgcttctcc gctgcggatg cagagcggga
    15001 tgccgacgcc gcgataggcg ttgccggcca gagcgaaatg cggaatcgcc gcggagcggg
    15061 cttcgatctg cgagaccagg tccagatgcc ccacgtgata ctgcggcatc atcccttgcc
    15121 agcggatgac ctcgaagaat tccggctcgc ccgacaggcc gatcagctcc cgcagctctt
    15181 caagcaccag tcggcgaatc tcctcctcgg gcaattgccc cagctccggc tggagcgctc
    15241 cgcccacgaa cacgcggagg agcactttgc catccggggc tcgcccggca aactttacgc
    15301 tggccaggct gcccgcgatg atccgccggt tctcgatcgc cggcacgaca aacccaaagc
    15361 catcgagcgg atgcgacgcc tgttccttgc ggatacccag caccgcgatc gagcaacccg
    15421 catgcggaat ccgcccgatc agcgctgaca gctcgtcatc gacgccggcc agcagacgcg
    15481 cactggcggc tcccggagca gcgaggatca gatcatcaaa ctcctccgcg cctccgccgc
    15541 cgagcgacac tcgccagcat ggcgagcggg ggccgtcagc gacccgatgc ggctcaattc
    15601 gcaccaccag acaactcagc cgcacgcacc ccgtcggcag ctttgccgca atcgcctcca
    15661 ccagctgctg catccccagc cgcggcgcaa caaacaatcc gtagcgagcg ccgctcgccg
    15721 cgtcctcagc gggctgacgc ccaccgctcg ccaattcccg actcccgact cccgactccc
    15781 gactcccgac tcccgccctc atcccgcgaa tcaagctccc atgtttccgc tccatcgcca
    15841 caaactgcgg cagcgtcgcc gccatgctga gctgcgcggg gtcggccgtg taaatgccgc
    15901 cgatcagcgg ctgaatcagt cgctcgaacg cttcgcggcc gaagcggcgg cagacgaacg
    15961 actccaggct ttcgtcgctg gtgtccgcgc ggcggggcgt caaatactcg gcggccagtc
    16021 gcagcttgcc gagcgggctc aagagcggcg tcgtgagaat cggccagatt ttggcagggc
    16081 tcatcagcgt gaagccctcg gggatcggca ccagcttccc ccggcgaacg acccacgagc
    16141 ggcgatgctg cgtattcgtg ccgattagtt catcggcgag gccgatgcga cggcaaaggt
    16201 ccactgcctc cggctcgcgg gtgatgaaca tgtccgccga gcgttcgatc agccagccgt
    16261 cgcgccgaac ggattgcagc gcgccgccca gccgctcgcc ggcttcaaag agggtcacgt
    16321 gcgactgcgg cgaaagctcg gtcaggcggt gagccgccgc gaggccggaa atgcctccgc
    16381 cgatgacggc gacgcggcga atgaggccgg agggagcggc gtagagaggg ggggacggag
    16441 cgacggcggg catgatgcca ttctactttg gccggctgtc ggtgctgtcg tctgggacgc
    16501 agtgaaatca gttgccgaag tcggcggagc ggttgatact atgggatcag gcctggccgc
    16561 cgcaaaatgt ggcggtgcac ttcctcccgc cgactgcctc ccgccgatgt atcgttgcct
    16621 gagcctactt gcgacggccg ccctcgcgct cgctgctgcc aacgcggctg ccgcggaagt
    16681 ggatttcggc cgcgaagtgc agccggtgct ggcggcgaag tgcgtccgct gccacgggcc
    16741 ggagaaacgg gaaaacggcc tgcggctgga tgcggggcgg acgatactga agggaggaga
    16801 caacggcccg gcggtcgtcg cccgcaagtc ggctgagagc ctgatgatcc tggcggtgac
    16861 gggaaaaacg gacctcgtct ccaagatgcc gcccaagggc gagccgctga ccgaggcgga
    16921 gctcgcactc atccgacggt ggatcgacga gggggcgacg ttctccagtg aggcggtcgc
    16981 cgcgaccaag agcaaacact ggtcgttcca gcggctcgtg cggccgagtc agccacttct
    17041 ccgtcaggcc gcgtggcctg caggagcaat cgatgcgttt ctgctcgcgc gcctcgaaaa
    17101 ggaaggtctc gcaccatcgc caccggccgg ccggccgacg cttatccgcc gggcctatct
    17161 cgacctgatc ggcctgccgc ccgcaccgga tgccgttgat gcgttcgtga acgacgagcg
    17221 gcccgatgcc tacgagcggg ttgttgatca gctgctggct tcgcctcact acggcgagcg
    17281 ctggggccgc cactggctcg acctagcccg ctatgccgac agcaacggtt acacgcgcga
    17341 cttcggacgg cagatctgga agtaccgcga gtgggtcatc gccgcgatca accgcaatca
    17401 gccgttcgac gagttcacga tcgagcagct ggcgggggac atgctgcccg cggccacgca
    17461 ggagcagctc gtggcgaccg ggtttcaccg caatacgctg ttcaacgagg aaggtggcac
    17521 cgatcccgag cagttccgcg tcgaggcggt cgtcgatcgc gtcaacacaa ccgggtccgt
    17581 ctggctgggg ctgaccgtcg gctgcgcccg ctgccacacg cacaagtacg atccgctgtc
    17641 gcaggtcgag tactaccagc tgtttgccct gctgaataac tgcgacgagc cgacgatcga
    17701 agccccgtcg tcattgcagc gcgagcgagg ggagctcgcg ctccgcgacg agattcgcgg
    17761 ccagatcaag cagctggaac tgcgcctgga ggaagagcgg cccgacctgg aagccgcgca
    17821 gcgggcctgg gaggcgaccg tgacgccgca gcagcgggcc cgcctgcccg gccctgtgca
    17881 agtcgcttac gacatgaagt ttgagaaacg cgacgagaag aaccgccagc tcatcgagga
    17941 gtatttccgg caggccgacg tcgcccgcga gcagtttccg ctcctgcagg aggtattcga
    18001 gctgcgcgaa gtcgagccca agattccaac gacgatgatc ctccgagaac gcaaggagcc
    18061 gcgggaaacg ttcgtccata agcggggcga tttcctcgac cgtgggccgg atgtgaccgg
    18121 cggcgtgccc gcagtgctgc cgccgctgca atccagcggc gagcgggcca cgcggctgga
    18181 ctttgcccgc tggctcgtct cggccgaaaa cccgctcacg ccgcgagttg tcgtgaatcg
    18241 cgattggctc cagttcttcg gccgcggcct ggtcgaaacc gaggacgact ttggcacaca
    18301 gggcacgcca ccgacgcatc ccgagctcct cgactggctg gcggtcgagt tcagccgcgg
    18361 ctgggattcg aaagcacttc acaggcggat cgtcacgagt aacgtctatc gccagtcgtc
    18421 ggccgtgcgg agcgatctga gcagccgcga tccacagaac aagctgctcg cgcggcaggc
    18481 ccgcctgcgg ctggacgccg agatcatccg cgacgtggcc ctgtcggcgg cgggcctgct
    18541 gacgacgcag ctcggcgggg cgagcgtgtt tccgccgcag ccggaaggtg tgttcgactt
    18601 cacgcaggat cccaagccgt ggctggcggc caagggcggc gaccggtatc gccggggcat
    18661 gtacacgcat ttctggcgct cgagccccta tccgatgctg atggccttcg acgcgccggc
    18721 ggccaacgtg acctgcaccc gccgcctccg cagcaacacg ccgctccagt cgctgacgct
    18781 ggccaacgac caggcgtttt acgagtgcgc ggcggccttg gccgagcgag tttgcaccgc
    18841 aacggccgat ccgtcatcgc gcgcgaacct ggcgttccgc ctctgtctgt cccgcgagcc
    18901 aacggccgcc gaagcggaac tgctgggccg ccttgtggag cgcgagacga cgagcacccc
    18961 cagcgatccg gctgctgcct ggacccgcat ttgccgcgtg ctgctaaact tggatgagac
    19021 gatcacgcgc gaatagacgc atgcagaact ccgcactccg tattccgaat tccgctttgc
    19081 taagcgctcg ccggcacttc ttccagcagt gccgcgtggg cctgggcgcg atggcgctgg
    19141 gatcgctcgt ggcgggagag cggagcaggg cggcggaagc atctgacccc ctggccgcgc
    19201 ggccgcccca tttcgcgccg cgggccaaga gcgtgatctt cctgttcatg gccgggggcc
    19261 ctagccagct ggaaatgttt gagcccaagc cggacttgca aaagctggat gggcaggtga
    19321 ttcccgagtc gttcgtgaag accaagcggt ttgcgttcat caagcaggat gccaagctgc
    19381 tcggcacgaa gcgcacgttc gcccaatacg gcgaaagcgg gcagacgatc tctgagtgcc
    19441 tgccgcacct ggccggcgtc gccgatgaca tttgcacgct gcgggcggtg aagaccgacg
    19501 tcttcaacca cggcccggcc aagttcttcc tcaatacggg ctcgccgctg ttcggccggc
    19561 cgagcatggg agcctggatc acttacggca tcggcagcct gtcgcgcgac ctgcccggtt
    19621 tcgtcgtgct gcagtccggc ccccgcgggc cgcggggcgg tgcgccgaac tggggcagcg
    19681 gctttctgcc gacgacttat cagggcgttc ccttccgcag caccggcgcg ccgatcctca
    19741 atctggccag cccggaaggc accagcccgg acgatcagcg gcggttcatc gagtcggtca
    19801 acgagttcaa ccgcctgcgg ctggccgaca ccggcgacgg cgagatcgcc acgcggatcg
    19861 ccgcctacga aatggccttt cgcatgcaat ccagcagccc ggagctgatg gacctgtcgg
    19921 gcgaatcggc cgagacgctg gccctctacg gggccgaagc cggcaagcca tcgttcgcga
    19981 tgaactgcct gttggcccgc cggctggtcg agcggggcgt gcggttcgtg cagctctatc
    20041 acaccgactg ggaccaccac ggcaacaagg gcaccgagct tggcgaatcg ctcgacgcga
    20101 tctgcaagca gaccgatcag ccggcggcgg ccctggtccg cgacttgaag cggcggggtc
    20161 tgctcgatga gacgctcgtg gtctggggcg gcgaattcgg ccgcacgccg caggggg
//
LOCUS       DASRJB010000040         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2553079, whole genome shotgun sequence.
ACCESSION   DASRJB010000040 DASRJB010000000
VERSION     DASRJB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553079"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(669..2693)
                     /gene="glmS"
                     /locus_tag="VFB80_01130"
     CDS             complement(669..2693)
                     /gene="glmS"
                     /locus_tag="VFB80_01130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZN32381.1"
                     /translation="MCGIVGYVGPRRADEILLEGLRRLEYRGYDSAGLATLTPDGQFH
                     VAKSVGRIDALAEKIAAAPLAGTTGIGHTRWATHGAPTEANAHPHFGVARDSVAGSPA
                     VVAWSPDHATGATAGLQKVRGQETLAQPAALRDASISRSEMPTLALAHNGVIENYLPL
                     KEKLLAEGYQFSSSTDTEVIAHLIVRSLAKMEARRSEFPADEPYAVVLAAVTHALGKL
                     RGTYGLVILFREHPELIVAARQGSPLVVGVGQGEHFVASDASPLAGRTGKIVYLADHQ
                     IALVTADSLEVRHREHGRIEHLVHDLAASETDVDLGGYPHYMLKEIFEQPEAIENALR
                     GRLNDQHATAVFGGLNLTPQQLRGASRILLTACGTSWHAALVGEYLIEELARIPVEVE
                     YASELRYRNPPLEPGTLLFAITQSGETADTLAALREIKRKGHPTLALCNVVGSTIAQE
                     ADGGVYLHAGPEIGVASTKAYASQVFTLAMLALYFGRLRHLSFEAGQRIIAQLRQLPD
                     RLRQTLDVHAAVRRIAAKYCHATNFLYLGRQYNFPTALEGALKLKEISYIHAEGYPAA
                     EMKHGPIALVDEHTPSVFIMPQGSVYDKVMSNLEEIKARGGPVIAIAPEGDRAVARLA
                     DDVIYVPRVEEFLSPLVNIIPLQLLAYHIAILRGCDVDRPRNLAKSVTVE"
     gene            complement(2786..>3251)
                     /locus_tag="VFB80_01135"
     CDS             complement(2786..>3251)
                     /locus_tag="VFB80_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32382.1"
                     /translation="EPAVPQPSRAPLIIERPAAPMAVEPPAAKEPTPDPPEPPPPPAT
                     PPSPPENATPELEAGWLTIVNPPDTGGPVHLTVEGVVYRLAPGEYCQVAAGGARRVAY
                     HQGDDFGYARHDLARGTHAFEIGNTGWTLKPLTPAAARQLVEHCRLRTAERE"
BASE COUNT          494 a         1027 c         1121 g          609 t
ORIGIN      
        1 gcgtgtggat ttttcgcaag attgttccaa actcttgccg cgtcgggttt tgcgttgtcc
       61 agctcgggca atctttcgcc ctccaggccg gctatcccgc cgaacctggt gtataatcat
      121 gtccacgttg tgtctcgccg ccagcccgcc tgctcgccgg ttcggaaatg tagccgcgga
      181 tggacgcgga taaaacggat cggaaatagc tctgtctgat ccgtgtcatc cgcgttcatc
      241 cgcggctatt cttacccagc tatcgcggct cgttggtgaa tcaatccgga gccaaagcgg
      301 gaaagtcgcg gagtttctgc cgaatgtgtc caggatgttt caccacagtg attcggcgcg
      361 ggtctccgaa ggcctcaccc gcgctgcgac tctggcactc acactgggtg gcacggtcgg
      421 gacggaatgg tggcgaattg ttcgccacgc ggtttttgtc gcaagtgtta gcagggactc
      481 gacttgccgc caaaacgaat gagtttttcg ccagttcccg aggaatcgcg cctctttggc
      541 gatttattcg ccagtcaatg tgtgaaaaac ccctgagttt tcgcgaaagt gagccggaat
      601 ttttcgccac agagcctgtc catgcggcga acattgtggt ctgcagccag aggcgaccgc
      661 cccaccactc actccaccgt cacgctcttg gccaggttcc gcggccgatc cacgtcgcag
      721 ccgcggagga tggcgatgtg ataggcgagc agctgcagcg ggatgatgtt caccagcggt
      781 gagagaaact cttcgacccg gggaacgtag atcacgtcgt cggccaggcg ggccacggcg
      841 cggtcccctt cgggggcgat ggcgattacc gggccgccgc gggccttgat ctcctccagg
      901 ttcgacatga ctttgtcgta caccgacccc tgcggcatga tgaacacgct gggcgtgtgc
      961 tcgtccacca gggcgatcgg gccgtgcttc atctcggcgg cgggataccc ctcggcgtgg
     1021 atgtagctga tctccttgag cttgagcgcc ccttccaggg cggtcgggaa gttgtactgg
     1081 cggccgagat agaggaagtt cgtcgcgtgg cagtacttcg cggcgatccg ccggacggcc
     1141 gcgtgcacgt cgagcgtctg ccgcagccgg tcgggcagct ggcggagctg ggcgatgatc
     1201 cgctggccgg cctcgaagct gaggtgccgc agccggccga agtacagagc gagcatcgcc
     1261 agcgtgaaga cctgcgaggc ataggccttg gtcgaagcga cgccgatttc ggggccggcg
     1321 tgcaggtaaa cgccgccgtc ggcttcctgg gcgatggtgc tgccgacgac gttgcacagg
     1381 gccagcgtgg gatggccctt gcgcttgatc tcgcggaggg cggccagcgt gtcggccgtt
     1441 tcgccgctct gggtgatggc gaacagcagc gtgcccggct cgagcggcgg attgcggtat
     1501 cgcagctcgc tggcgtattc gacctcgacc ggaatgcggg ccagctcttc gatcaggtat
     1561 tcgccgacga gggccgcgtg ccagctcgtc ccgcaggccg tgagcaaaat gcggctggct
     1621 ccgcggagct gctggggcgt gagattcagg ccgccgaaca ccgcggtggc atgctggtcg
     1681 ttcaggcgtc cgcggagtgc gttctcgatc gcctcgggct gctcgaagat ctccttgagc
     1741 atgtagtgcg ggtagccgcc gagatcgacg tcggtctcgc tggccgccag gtcgtgaacc
     1801 agatgctcga tccggccgtg ctcgcggtgc cggacctcga gggaatcggc cgtgacgagg
     1861 gcgatttgat ggtcggccag gtaaacgatc ttcccggtcc gtccggcgag cggcgaagcg
     1921 tcgctggcga cgaagtgctc gccctgcccg acgccgacga ccagcgggct gccctgccgg
     1981 gcggcgacga tcagctccgg gtgctcgcgg aacagaatca ccaggccata agtgccgcga
     2041 agcttgccaa gagcgtgcgt caccgccgcg agcacgaccg catacggctc atcggccggg
     2101 aactcgcttc gccgggcctc catcttcgcc aggctgcgga cgatcaggtg ggcgatgact
     2161 tcggtgtcgg tcgagctgct gaactggtag ccttccgcga gcagcttttc cttgagcggc
     2221 aggtagttct cgatcacgcc gttgtgggcc agggccaaag taggcatctc gctccgcgag
     2281 atgctggcgt cacgcagagc cgccggttgt gcgagggtct cctgaccccg caccttttgg
     2341 agacctgcgg tcgcgccggt ggcatggtcg ggagaccatg ccacaacagc agggctgccc
     2401 gcgacggagt cgcgggctac gccaaaatgc gggtgggcgt tggcttcggt cggagcgccg
     2461 tgggtggccc accgcgtgtg gccgatgccg gtcgtgccgg ccagcggcgc ggcggcgatc
     2521 ttctcggcca gcgcgtcgat ccggccgacc gatttggcga cgtgaaactg cccgtccggc
     2581 gtgagcgtgg ccagcccagc gctgtcgtag ccgcgatact ccagccggcg gaggccctcc
     2641 agcagaatct cgtctgcccg gcgcggtccg acgtaaccta cgatgccgca catacaggac
     2701 gcctcctggt cttggcgatc aatcctttcg ccccgccgct atccgattct aggcgactct
     2761 ggcgcttggg ccagtgcagt ctgcactact ccctttccgc tgttcgcagc cggcagtgct
     2821 ccaccagctg acgggcggcg gccggtgtga ggggcttgag cgtccagccg gtgttgccga
     2881 tctcgaaggc gtgcgtgccg cgggcgaggt cgtgacgggc gtagccgaag tcgtctccct
     2941 ggtgatacgc gacgcgccgc gcgccgccgg cggcgacctg gcaatactca cccggcgcga
     3001 ggcgatagac gactccctcc acggtcaggt gcaccgggcc gcccgtatcc ggcggattga
     3061 cgatcgtcag ccatcctgct tccagctccg gcgtcgcatt ttccggcggc gatggtggag
     3121 tggctggcgg cggcggcggc tctggcggat caggagtcgg ctctttggcc gcaggcggtt
     3181 caacggccat gggcgccgct ggccgctcga tgatgagcgg cgccctggac ggctgcggga
     3241 cggccggttc c
//
LOCUS       DASRJB010000041        10592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_13868, whole genome shotgun sequence.
ACCESSION   DASRJB010000041 DASRJB010000000
VERSION     DASRJB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13868"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFB80_01140"
     CDS             complement(<1..405)
                     /locus_tag="VFB80_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32383.1"
                     /translation="MSSKLSYLLVLFALAGAVPLTAMAAGPASKSKEPANSVGLFEGM
                     DAGNIEVKIIPKDDKEGTITIKNKTDKPLTIKLPEAFAGVPVLAQGLLGGGNAGGGNN
                     NNGGGGNQGVGGGMMGGMGGGGMMGGGGFFNVE"
     gene            656..2818
                     /locus_tag="VFB80_01145"
     CDS             656..2818
                     /locus_tag="VFB80_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32384.1"
                     /translation="MAHRWHYLPIGLVISGVLAVCAAAAANHAALGWWQALIYAGCAA
                     AAGATLLFRRKLGLVEVELDQFRRRLAAEEDRLNEQRSQLEVLRRTVEDELQSQASRL
                     DKREQALADRLVTYHEWMEFPQPTELSQPAAASDAELAALARKDRELQELLKAETKSL
                     YDRILQNKYAPQGQVLLPVIRDDVLALVLRVARIYQPTIEQPLLEASLPRVLRGISRA
                     SLQLLVVLDELPVNIQGASLNTLYGYVRNAVSAWRMYKSAEPYWPYVNTAYYLGRFAL
                     GANPITLGAWWFVSSLGTRGAQAIAQHVINRQALELLSSLVRVIGYEVAGIYGGDIRH
                     RDANWIYAAELTELVSQFPLSRDSLAHALREVGTLQLRSEYDRAFLYRCLAGRKSAHP
                     EQYSPAKVLSMEERRAAATRLERFLEAFIHGKSADRVAQWKSAAEERLGVKLSVSLKV
                     SPATVRDQIVDAVRSLASFLVSIKQLEPPEAVKLLAESTVLAQLPAEERPKLLEELAA
                     GASYFFEHADLDPDGDLVEKYLDDLAALHAAVPPRGAGIEETLADVAAYLRRPPKKMH
                     ALAEKHYAARLAQRMIGDPARKIPPQVARAALDLLVDPAEPARLLLGPATLEWPDGGR
                     PETVAGGELWLLGVDQRLILFAAAEQPQVIWRGTPRDVHAEQARQLLGSSCRLTGGQW
                     QTNGRSQPLAIRLPAALMSSYAGQFRPLLEMLGNSPKH"
     gene            complement(2865..3635)
                     /locus_tag="VFB80_01150"
     CDS             complement(2865..3635)
                     /locus_tag="VFB80_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN32385.1"
                     /translation="MNGKTLKEKLVRGETVFGTFFQHAVTPAIVDFLPEGALDFVIVS
                     AEHNALELAEFLPIRYALASKGIACLARIHSREPDDVSKACDSFDGVVVPYVEDVEHA
                     KRLAAAAVYRPLKGKALATVLAGGAWPSDKTRQYVTEVKCAGTIFVPMIESVLAVQNL
                     DAICSIPGVSAVFVGPNDLTTNMGIPNEYDHADLVATLKTVIDTADRHHVAAGCWFGK
                     LEQMQRTIRQGARFNVFSNDSSLLQEAMQASFSVLRKK"
     gene            complement(3657..5138)
                     /locus_tag="VFB80_01155"
     CDS             complement(3657..5138)
                     /locus_tag="VFB80_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN32386.1"
                     /translation="MKPLLAIALAAALAAGRLPAAEARPNILWLIGENLSHDLGCYGA
                     KNVRTPHLDRLAAEGVRYTRVFATNPACAPSRSAFFTGMYQTSIDVHAMRSHRSDDFR
                     LPAGVRPLTHRLREAGYFTANLKTIGSLAIGTGKLDLNFVNEGPLYDEAAGDWSVLAA
                     RQPFFAVVNSHEAEYDIYDRRSAEKPRVEWVGEREHEPIATEGNVTPPPYYPDHPVVR
                     QEWARYLNSVSGMDRRIGWVLERLRADGLADSTVVIFFGDNGRLEPRGIHWCYDCGLR
                     VPLIIRWPPNFPAPPQLRPGSVDERIISLVDLTATTLWVAGLPQPPLMQGRPFLGSDA
                     APDRPFAFAARDRIDETVQRIRSVHDARFHYIRTYTAGPTFASLNRYKEKCFPILPLM
                     RQLHAAGKLSGPPAALLELRGPCEELFELASDPHEIHNLAGSAVPEHREALVRLRAAL
                     DAWIAETGDTGQRPEPAQVVAPFEREMHDWFGTPAWHRIPAAP"
     gene            complement(5147..6487)
                     /locus_tag="VFB80_01160"
     CDS             complement(5147..6487)
                     /locus_tag="VFB80_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN32387.1"
                     /translation="MSPDALSSRRRLLLGSLAGATAGWLASRLPAEDRPAITRPRATS
                     GDTVSEPDWNERLTITVGPQDADLVGTTDKVLQAAVDYVARLGGGTVKILPGTYRLRN
                     SVFLASKVRLLGSGSDSILIKEPSEVAKLAEDSDWFDQEITLEGGGGFRVGDGICLRT
                     KKPDGGGRDVLKRTLVAKDGNRFKLDRALRENFWLMGGATCASLFPILSGENIADLAI
                     ENLALDGNKERNENLDGNYAGCIFLQDCSRVAIRGVEARNYNGDGVSWQVCHDVTVEN
                     CHSHDHAGLGLHPGSGSQRPTIKSNRLERNDIGLFFCWGVKYGLAQGNQISDNRSYGV
                     SIGHRDTENLIVDNDVVRSGKVGVLFRPERGKDFAPHRNRVERNRIVDSGPESGTAVD
                     VQGETQEVAIVGNDIRESRGAAQRIGIRLGKSTKQIKVEENRFAGLMKEVEDLR"
     gene            6633..8009
                     /locus_tag="VFB80_01165"
     CDS             6633..8009
                     /locus_tag="VFB80_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32388.1"
                     /translation="MHRIHLAATLLLVMAMASLAAHAADAPAIRIATFDVDATPPVGS
                     AMAYDPVKRLDELTLRCRGIVLLGAGQPIVLCAVDWIGIANEGHDAFRDGLAEAAGTT
                     RDRVAVHTLHQHDAPGCDFTAERIVRELGLAEYGRFDGTFHRQVIQRAAAALKAALPA
                     AQEVTHCGFGAAEVKEVASNRRIKGPDGKVRATRYTATRDPALRAEPEGVIDPQVSLV
                     SFWNGEKLLAALSYYACHPQSYYRTGVPSPDFPGIARWMRGQAVPEALHVHFNGAGGN
                     IGAGKYNDGAKENRLILATRLADGMQRALAATQKQPLAAGDVGWQTVPVRLPPAPHLK
                     EADLLQALRTEPARGYIAKADQLAWLRRCQAGHAINVACLRLGSTRVLHLPGELFVEY
                     QLAAKAMRPDLHVALAAYGDYGPGYIGTAAAYPEGGYETGPGASNVAPEVEQVLTEAM
                     KRLLQATP"
     gene            8075..8656
                     /locus_tag="VFB80_01170"
     CDS             8075..8656
                     /locus_tag="VFB80_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32389.1"
                     /translation="MKSNTDKALLHSVERNDRALLFKARWDRGTARQVVPPLIRVLES
                     HDASLVLRALSAFLCIGPEAHSAARSIAKLLTVADERIFNTAAIALAAVSLKKPKLAV
                     SPLLEMSKDADRLKYAMFGLVELGPGAKSAGGVFVRAYDSRDSRIRRLALRGLKELGV
                     DARTAVPVLKRALSDSSQEIRKYAKKILATSYD"
     gene            8663..10054
                     /locus_tag="VFB80_01175"
     CDS             8663..10054
                     /locus_tag="VFB80_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32390.1"
                     /translation="MFTSPHFRLSRRACLRSLVGGSLLLPGILSELAAADAPPADPLT
                     PKQPHFPAKAKRVIFVFSSGGVSHMDTFDYKPELFKADGKITGAGGGLSNQQKPLVKP
                     RWEFRPGGECGTRVSDLFPHVRDCLDDLCVINSMKSDDNEHYQATLAMHTGSFFFTRP
                     SFGAWLSYGLGTVNQNLPSYVVLAPYFPYGGGQVWGNDFLPAYHQGTRVVPGADPIPN
                     LARRTKQAGLQELELDLAEALNKKHLAASGNDSDLAARIRSFETAFHMQAAAPEAFDI
                     SRESDETLGLYGLKRGDTESFAWQCLVARRLAERGVRVIELIDRGAADNWDAHQDMAQ
                     HEPLAKAIDRPLSGLVRDLKRRGMLDETLVVWTTEFGRTPGQDGVKGRGHHGACFSSW
                     LAGGGIKGGITYGQTDPIGATVAADLCHVHDFHATILHLLGIDHTRLTYRHGGRDYRL
                     TDVSGNVVTKILA"
     gene            complement(10337..>10592)
                     /locus_tag="VFB80_01180"
     CDS             complement(10337..>10592)
                     /locus_tag="VFB80_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HZN32391.1"
                     /translation="LGIGTAQTALAYGADDIDGTVRHELIYHDAGATTPELLTTEQIR
                     RLIVEAGREPIERDTLYRRIVRDPAQPARWTVGEELLVSA"
BASE COUNT         1777 a         3519 c         3525 g         1771 t
ORIGIN      
        1 ctcgacattg aagaagccgc cgccacccat catgccgccg ccgcccatgc cgcccatcat
       61 gccgccgccg acgccctgat tgccgccgcc gccgttgttg ttatttccgc cgccggcgtt
      121 gccgccgccg agcaggcctt gagccagcac gggaaccccg gcaaaggcct ccggcagctt
      181 gatcgtgagc ggcttgtcgg tcttgttctt gatcgtgatc gtcccttcct tgtcgtcctt
      241 gggaatgatc ttgacttcga tgttccccgc atccatgcct tcgaacagac cgacgctgtt
      301 agccggctct ttggacttgg acgcgggacc cgcggccatg gcggtcagtg gaacagcgcc
      361 ggccagggcg aagagcacca gtaaatagga gagcttgcta ctcatgagaa cgactccatg
      421 caatcagaca gatttcgccc gaagcaacga actgacagag tttgccggcc ccaattcgtt
      481 gcgcaccatc aggataatcg cccgcggccg cgtttccaag cagaaagaat agggaaattc
      541 tggggaaaac gcggagtgaa gccttcctcg gcctacccaa acccggcaat tcccaggccg
      601 aagagcgaag tacaatttgt gccggcggct ttccacgccg acggctgcta tcaccatggc
      661 gcatcgttgg cactacctcc cgatcgggct agtaatctct ggggtactgg cggtctgcgc
      721 ggccgcagcc gccaaccacg ccgcgctcgg ctggtggcag gcgctgatct atgccgggtg
      781 tgccgcggct gcgggggcga ccctgctctt tcgccgcaag ctcggcctgg tggaggtcga
      841 gctggaccag ttccgccgcc gtctggccgc cgaagaggac cggctgaacg agcagcggag
      901 ccagttggaa gtcctccgcc gcacggtcga ggacgagctt cagtcgcagg ccagccggct
      961 cgacaaacgc gagcaggcgc tggccgatcg cctggtgact taccacgagt ggatggagtt
     1021 cccgcagccg acggagttgt cgcagccggc tgccgccagt gatgccgagc tggcggcact
     1081 ggcccgcaag gaccgcgagc tgcaggagct gctgaaagcc gaaacgaaat cgctctacga
     1141 ccgcatcctg cagaacaagt acgccccgca gggccaggtg ctgctgccgg tgatccgcga
     1201 cgacgtgctg gcgctggtgc tccgcgtggc ccgcatctat cagcccacca tcgagcagcc
     1261 gctcttggag gcgagcctgc cgcgggtgct gcgggggatc agccgggcga gcttgcagct
     1321 gctggtcgtg ctcgacgagc tgccggtgaa cattcagggg gcgagcctga acacgctgta
     1381 cggctatgtc cgcaacgcgg tttcggcgtg gcggatgtac aagtcggccg agccctattg
     1441 gccgtatgtg aacacggcgt attacctggg ccgcttcgca ttgggggcca acccgatcac
     1501 gctgggggcc tggtggttcg tcagctcgct gggcacgcgc ggcgcccagg caatcgccca
     1561 gcacgtgatc aaccggcagg cgctggagct gctcagcagc ctcgtccgcg tgatcggcta
     1621 cgaagtcgca gggatctatg gcggcgacat tcgccaccgc gacgcgaact ggatttacgc
     1681 cgcggagctg accgagttgg tcagccagtt cccgctctcg cgcgacagcc tggcccacgc
     1741 cctccgcgag gtcggcacgc tgcagctccg cagcgaatac gaccgggcgt ttctctatcg
     1801 ctgcctggca ggccgcaaga gcgctcaccc cgagcagtat tcgccggcca aagtgctctc
     1861 gatggaagag cggcgggcgg cggccacgcg gctggagcgg tttctggagg cgttcattca
     1921 cggcaaatcg gccgaccgcg tcgcgcagtg gaagtcggcg gccgaagagc ggctgggcgt
     1981 gaagctgtcg gtgtcgctca aggtttcgcc agccaccgtg cgggaccaga tcgtcgatgc
     2041 ggtgcggtcc ctggccagct ttctggtttc gatcaagcag ctggagccgc ccgaggcggt
     2101 caagctgctg gcggaatcga cggtgctggc ccagttgccg gccgaggagc ggccgaaatt
     2161 gttggaggaa ctggcggccg gggcatcgta tttcttcgag cacgccgacc tcgatcccga
     2221 cggcgacctg gtcgagaagt acctggacga cctggcggcg ctgcatgccg ccgtgccgcc
     2281 gcgcggagcg ggaatcgaag agacgctggc cgatgtcgcg gcctacctcc gccggccgcc
     2341 gaagaagatg cacgccctgg ccgagaagca ctacgccgcg cggctggccc agcggatgat
     2401 cggcgatccc gcccggaaga ttccgccgca ggtggcgcgg gcggcgctgg acctgctcgt
     2461 cgatccggcc gagccggccc gactgctgct cggtcccgcg accttggagt ggcccgacgg
     2521 cggacgcccg gagaccgttg ccggcggcga attgtggctg ctgggcgtcg atcaacggct
     2581 gattctgttc gcagccgccg agcagccgca ggtgatttgg cgtggcacgc cgcgcgacgt
     2641 gcacgccgag caggcccggc agctgttggg cagcagctgc cggctgaccg gcggccagtg
     2701 gcaaaccaac ggccgctcgc agccgctggc gatccgcctg ccggctgcct tgatgagcag
     2761 ctacgccggg cagttccggc cgctgctgga aatgctcggg aattcgccca agcattgaca
     2821 acgccgagac atgccaatgg acttgcttgc gatgcactta gttttcactt tttgcggagc
     2881 acggaaaacg acgcctgcat cgcctcctga agcagcgacg aatcgttcga gaagacgttg
     2941 aaccgcgccc cctggcggat cgtccgctgc atctgctcga gcttgccgaa ccagcagccg
     3001 gcggcgacgt ggtggcggtc ggcggtgtcg atgaccgtct tcagcgtggc gaccagatcg
     3061 gcgtgatcgt attcgttggg gatgcccatg ttggtggtca ggtcgttcgg tccgacgaac
     3121 acggcgctga cgccggggat cgagcaaatc gcatccaggt tctgcacggc cagcaccgat
     3181 tcgatcatcg ggacgaagat cgtacccgcg cactttactt cggtgacgta ctgccgggtc
     3241 ttgtcgctgg gccacgcgcc gccggccagc accgttgcca gcgccttgcc tttcagcggc
     3301 cgatagacgg cggccgccgc cagccgcttg gcgtgctcga cgtcctcgac gtatggcacg
     3361 accacgccgt cgaacgaatc gcaggccttc gagacgtcgt ccggctcgcg gctgtgaatt
     3421 cgcgccaggc aggcgatccc cttcgacgcc agggcgtagc ggatcggcag aaactcagcc
     3481 agctccagag cgttgtgctc cgcgctgacg atcacgaagt cgagcgcgcc ctcgggcagg
     3541 aaatcgacga tcgccggcgt caccgcgtgc tgaaagaacg tgccgaacac ggtctcgccg
     3601 cggaccagct tctctttgag ggtcttgccg ttcatgcgtt ggctcctggc gacgatttac
     3661 ggtgctgctg ggattcgatg ccacgcgggc gtgccaaacc agtcgtgcat ttcacgctcg
     3721 aagggcgcga cgacttgcgc cggttcgggc cgctggccgg tatcgcccgt ttccgcaatc
     3781 caggcgtcca gcgcggcacg cagccgcacg agcgcttcgc ggtgttcggg gacggccgag
     3841 ccggcgaggt tgtggatttc atgcgggtcg ctcgccagct cgaacagctc ctcgcacggg
     3901 ccgcgcagct ccagcagcgc agcaggcggc ccggagagct ttccggcggc gtgcagctgg
     3961 cgcatcagcg gcaggatcgg aaagcacttt tccttgtagc ggttcagcga tgcaaacgtc
     4021 ggtccggccg tgtaggtgcg aatgtaatgg aaccgggcgt cgtggaccga gcggatccgc
     4081 tggacggtct catcgatgcg atcgcgggcc gcgaacgcga atgggcgatc gggcgcggcg
     4141 tcggaaccca ggaaggggcg gccctgcatc agcggcggct gcggcaggcc cgcgacccaa
     4201 agcgtcgtgg ccgtcagatc gacgaggctg atgatccgct cgtctaccga tcccggccgc
     4261 agctgcggcg gcgcggggaa gttcgggggc cagcgaataa tcagcggcac gcgcagcccg
     4321 cagtcgtagc accaatggat gccgcgcggc tccagccggc cgttgtcgcc gaagaagatc
     4381 accaccgtgc tgtccgccag gccgtcggcc cgcaaccgtt cgagcaccca gccgatgcgg
     4441 cgatccattc ccgagaccga gttgaggtag cgggcccatt cctgccgcac gacgggatga
     4501 tcgggatagt acgggggcgg cgtgacgttg ccctcggtgg cgatcggctc gtgctcgcgc
     4561 tcgccgaccc attcgacgcg cggcttttcc gccgagcggc ggtcgtagat gtcgtactcc
     4621 gcttcgtgcg agttcacgac ggcgaagaag ggctgccgcg cggccagcac agaccagtcg
     4681 cccgcggcct cgtcgtagag cgggccttcg ttcacgaaat tcagatcgag cttgcccgtg
     4741 ccaatcgcca ggctgccgat ggtcttgagg ttggccgtga agtatcccgc ctcgcgcagc
     4801 cggtgcgtca gcggccgcac gccggcgggc aggcgaaagt cgtcactgcg atgcgagcgc
     4861 atggcatgca cgtcgatcga cgtctgatac atgccggtga agaacgcgga acggctcggg
     4921 gcgcaggccg ggttggtggc aaagacgcgc gtgtagcgga cgccttccgc cgccaggcgg
     4981 tccaagtgcg gcgtgcggac gttcttcgcg ccatagcagc ccaggtcgtg cgacaggttc
     5041 tcgccgatca accagaggat gttcggccgc gcctcggcgg ctggcaaacg gccggcggcg
     5101 agtgcagctg ccagggcgat cgcaaggagc ggtttcatgg gctcggctac cgcaggtcct
     5161 cgacttcctt catcaggcct gcgaagcggt tttcttccac cttgatctgc ttcgtggact
     5221 tgccgaggcg gatgccgatc cgctgggcgg cccctcgcga ctcgcggatg tcgttgccga
     5281 cgatcgcgac ctcctgcgtt tcgccctgca catcaacggc cgtgccggac tccgggccgc
     5341 tatcgacgat gcggttccgc tcaacgcggt tgcggtgcgg cgcgaaatcc ttgccgcgtt
     5401 cgggccggaa gaggacgccc accttgccgc tgcggaccac gtcgttatcg acgatcaggt
     5461 tctcggtgtc gcggtggccg atcgagacgc cgtaactgcg gttgtcggag atctggttgc
     5521 cctgcgccag gccgtacttc acgccccagc agaagaacag gccgatgtcg ttccgctcca
     5581 gccggttgct cttgatcgtc ggccgctgcg agcccgaacc ggggtgcagc ccgagccccg
     5641 cgtgatcgtg cgagtggcaa ttctcgaccg tcacgtcgtg gcagacttgc cagctcacgc
     5701 cgtcgccgtt gtagttgcgg gcctccacgc cgcggatcgc cacgcggctg caatcctgca
     5761 ggaagatgca gccggcgtag ttgccgtcga ggttctcgtt ccgctccttg ttgccgtcca
     5821 gggcgaggtt ctcgatcgcc aggtcggcga tgttctcgcc gctgaggatc ggaaacagcg
     5881 acgcgcaggt cgcgccgccc atcagccaga aattctcgcg cagggcccgg tcgagcttga
     5941 agcggttgcc gtccttggcc accagcgtcc gcttgagcac gtcgcggccg ccgccgtccg
     6001 gctttttcgt ccgcaggcag atgccgtcgc ccacgcgaaa accgccgccg ccctcgagcg
     6061 tgatctcttg atcgaaccag tcgctgtctt cggccagctt ggcgacctcc gacggctcct
     6121 tgatgagaat gctgtcgcta ccgctgccga gcaatcgcac cttcgacgcc agaaacacgg
     6181 aattccgcag gcgatacgtg ccgggcagga ttttcaccgt gccgccgccg agccgcgcga
     6241 cgtaatcgac ggctgcctgc aggactttgt cggtcgtgcc cacgaggtcg gcgtcttgcg
     6301 ggccgaccgt gatggtcagc cgctcgttcc agtcgggctc ggacacggtg tcgccggaag
     6361 ttgcccgcgg ccgcgtgatc gcggggcgat cctcggcggg caggcggctg gcgagccagc
     6421 cggcggtagc accggccagt gatccgagga gcaggcgtcg tcgcgacgag agggcgtcag
     6481 gggacatgtg tggacctcca attacgattg gatcgactgc caagcagaat atctcagccg
     6541 tcggcaaaac acactaaccc gacgcgccag cgagggaata agatgggtgc aagtccaccc
     6601 aatggactcc ttcagcttgg atggccacgc ccatgcatcg aatccacctc gctgcgacgc
     6661 tcctgctcgt gatggcgatg gcgagtctcg ccgcgcatgc cgccgatgcc ccggcgatca
     6721 ggattgcgac gttcgacgtc gatgctacgc cgccggtcgg atcggcgatg gcctacgacc
     6781 ccgtgaagcg gctcgatgaa ttgacgctgc ggtgccgcgg gatcgtgctg ctcggcgcgg
     6841 ggcagccgat cgtgctctgc gccgtggact ggatcggcat cgccaacgaa gggcacgacg
     6901 cctttcgcga cgggctggcc gaagcggccg gcacgacgcg cgaccgcgtg gccgtgcaca
     6961 cgctgcacca gcacgacgct cccggctgcg acttcacggc cgagcggata gtgcgggagc
     7021 tgggcctggc cgaatacggc cgcttcgacg gcacgtttca ccgtcaggtg atccagcggg
     7081 cggctgcggc gttaaaggcc gcgctccctg cggcacagga ggtcacccac tgcggcttcg
     7141 gcgcggccga ggtcaaggaa gtcgcctcga accgccgcat caaagggccg gacggcaagg
     7201 tgcgagcgac cagatacacc gcgacgcgcg accccgcatt gcgggccgag cccgaaggcg
     7261 tcatcgatcc gcaggtctcc ctcgtttcgt tctggaacgg cgagaagctg ctcgcggcgc
     7321 tcagctacta cgcctgccat ccgcagagct actatcgcac cggcgtgccc agcccggact
     7381 ttcccggcat cgcccgctgg atgcgcgggc aggccgtgcc cgaggcgctc cacgtccact
     7441 tcaacggcgc gggaggcaat atcggcgcgg gcaagtacaa cgacggcgcg aaagaaaacc
     7501 gcctgatcct cgcgacgcgg ctggccgacg gcatgcagcg agcgctcgca gccacgcaga
     7561 agcaaccgct ggcggccggc gacgtcggct ggcaaaccgt gcccgtccgc ttgccgcccg
     7621 cgccgcattt gaaggaagcg gacctgctgc aagcgctccg cacggagccc gcgcggggtt
     7681 acatcgccaa ggccgaccag ctcgcctggc tgcggcgctg ccaggcaggc cacgcgatca
     7741 acgtggcctg cttgcggctg ggcagcacgc gcgtcctgca cctgccgggc gagctgtttg
     7801 tcgaatatca gcttgccgcc aaggccatgc gtcccgatct ccacgtcgcg ctcgccgcct
     7861 acggcgacta cggccccggc tacatcggca ccgcggccgc ctatcccgaa ggcggctacg
     7921 aaaccggccc cggcgcgagc aacgtcgcgc ccgaagtgga gcaggtgctc accgaggcga
     7981 tgaagaggct gctgcaagcc actccgtaag tggagcatct gctctcttcg cctttgggag
     8041 agcgccggac gatgtcgtgt acgcgaagtg atcgatgaaa tcgaatactg acaaggcgct
     8101 tctccattcc gttgagcgaa acgaccgagc gttgctgttc aaagcccggt gggacagggg
     8161 cacggccagg caagtcgtgc cgccgctgat tcgcgtactc gagtcacacg atgcgtcatt
     8221 ggtgctgcgt gcgctatcgg ccttcctctg tatcggaccg gaagcgcaca gtgctgctcg
     8281 ctcgattgcg aagttgctta ccgtcgccga cgagcgcatc tttaacacag cagcgatcgc
     8341 cctggccgcg gtatcattaa aaaaaccgaa gctggcggtg tcgcccttgc ttgaaatgtc
     8401 gaaagacgca gaccgactga agtacgcgat gttcggcctc gtggaacttg gtccgggggc
     8461 taaatcggct ggtggcgtct ttgtaagagc atacgacagc cgcgattcgc gaatacggcg
     8521 gctggccttg cgcggtttga aagaactggg cgtcgacgcg agaacggcgg tacccgtgct
     8581 caagcgcgcg ttgtcggaca gttcacagga aatccgcaag tacgccaaga agatcctcgc
     8641 aacaagctac gactagactc agatgtttac ttcaccgcac ttccgcctct cgcgccgcgc
     8701 ctgcctccgc tcgctcgtcg gcggctcgct gctcttgccc ggcattctct cggagctggc
     8761 agccgcagac gccccgccgg ccgatccgct gacgcccaag cagccgcact ttccggcgaa
     8821 ggccaagcgg gtgatcttcg tgttctcgtc gggcggcgtg tcgcacatgg acacgttcga
     8881 ctacaagccg gagttgttca aggcggacgg caaaatcacg ggggccggcg gcggactctc
     8941 caatcagcag aagccgctgg tcaagccgcg gtgggaattc cggccgggcg gcgagtgcgg
     9001 gacgcgggtc agcgacctct ttccgcacgt ccgcgactgc ctggacgacc tctgcgtcat
     9061 caattccatg aagagcgacg acaacgagca ttaccaggcc acgctggcga tgcacaccgg
     9121 ctcgttcttc ttcacgcggc ccagcttcgg cgcgtggctg agctacggcc tgggaaccgt
     9181 gaatcagaac ctgcccagct acgtcgtgct cgcgccgtac tttccgtatg gcggcgggca
     9241 ggtgtggggc aacgattttc tgcccgccta tcaccagggg acgcgagtcg tacccggggc
     9301 cgatccgatt ccgaatctcg cccggcggac gaaacaggcc ggcttgcaag aattggagct
     9361 ggacctcgcc gaagccctca acaagaagca cctggccgcc agcggcaacg actcggacct
     9421 ggcggcccgc atccgctcgt tcgagacggc cttccacatg caggccgccg cgcccgaagc
     9481 cttcgacatc agccgcgaat cggacgagac gctcggcctg tatggcctca aacgggggga
     9541 caccgagagc ttcgcctggc agtgcctcgt cgcccgccgg ctggccgagc ggggcgtgcg
     9601 ggtgatcgag ctgatcgacc gcggggccgc cgacaactgg gatgcccacc aggacatggc
     9661 ccagcacgag ccgctggcca aggcaatcga ccggccgctg tcgggcctcg tgcgcgacct
     9721 gaagcgccgc ggaatgctcg acgagacgct cgtcgtctgg accaccgagt tcggccgcac
     9781 gccgggccag gacggagtga agggccgcgg gcatcacggg gcctgcttct cgtcctggct
     9841 ggccggcggc ggcatcaagg gcggcatcac ctacggccag accgacccga tcggcgcgac
     9901 cgtcgccgcc gacctgtgcc acgtccacga ctttcacgcc acgatcctgc acctcttggg
     9961 catcgaccac acccgcctca cctaccgcca cggcggccgc gactatcggc tcaccgacgt
    10021 cagcggcaac gtggtcacga agatcctggc gtagcagttt ggcgcggaaa tccagaatgt
    10081 gaacatttgg tacaatattt gcaccgctct tgctgtttgc gcagcggctg ccaatttggc
    10141 ctgacccatt tcgcccgacg ccaagcgggc cagccgttct ctaaccctta tagtcctaag
    10201 gcgaaaaact ggcctggcgt gcgacgcaaa catttgctgc gacagcactt gcgtctaatg
    10261 cttatgcccg cagccggcgc gaaaaaccga ggctcaaacg cttatgcagg agcgcagtcg
    10321 ctgcccaaag aagtcgctag gcgctgacta gcaattcctc gccaacggtc cagcgggccg
    10381 gctgagccgg gtcgcggacg atccgccggt agagggtgtc gcgctcgatc ggctcgcggc
    10441 cggcttcgac gatcagccgg cggatttgtt cggtcgtgag cagctccggc gtcgtcgccc
    10501 cggcgtcgtg atagatcaac tcgtggcgga cggtgccgtc gatgtcgtcg gccccgtagg
    10561 ccagggccgt ctgggccgtg ccgatgccca gc
//
LOCUS       DASRJB010000042         5253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_861848, whole genome shotgun sequence.
ACCESSION   DASRJB010000042 DASRJB010000000
VERSION     DASRJB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861848"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..1122
                     /locus_tag="VFB80_01185"
     CDS             553..1122
                     /locus_tag="VFB80_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN32392.1"
                     /translation="MLRRVFLALTVVALCGPAYAAEAEVGAKAPDLKGLIGTDGKEYN
                     LADMKDKIVVVCFTCNACPVAVGYEDRFIEFQKKYGSKGVKFIALNANKKTEDIAVMK
                     ARAEEKGFNFPYVYDKTGESATAYGARVTPHIFVLDQDRKVAYVGSFDDSQNNPKKQY
                     VSDAVDALLAGKKVEFTNSKAFGCGIQNK"
     gene            1215..1742
                     /locus_tag="VFB80_01190"
     CDS             1215..1742
                     /locus_tag="VFB80_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN32393.1"
                     /translation="MRFWIRFAVLGLASALAGCDYNAPLGSSPTPVASASEIQLPQGD
                     AKALQAIIDKHKGEVVFVDFWATWCGPCVEGFPHTVELSKKYKGQGLATIAVSFDSLE
                     NEGAVREFLKKQGADFDHLLSKYDGVSQDAAVDFDVEALPQYRLYDRQGKLRHKWEGK
                     SEEIEPAIQTLLAEK"
     gene            complement(1743..2996)
                     /locus_tag="VFB80_01195"
     CDS             complement(1743..2996)
                     /locus_tag="VFB80_01195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZN32394.1"
                     /translation="MPQPLNSTTRSLAMPPQLPAVQLKSVGLHPLIYRKRLARVDREA
                     APGDLVQVQDAAGQPTGYGLFNPKSELSLRMLSLGEPPDEAWWQRRLADAVRLRHETL
                     RLNEVTDAYRVVHAEGDGLSGIVIDKLCNVLSAECFSVGMYQRAEAILALLAPLLGTQ
                     HTLIRGGPASLAQEGFEGEAVASPQLPSRVTIQEFGTRFRVDFSSGHKTGFFCDQRDN
                     RKLLASFCGGKTVLDLCCYTGGFAIQAMRLGGAKEAIGVDLDAEPLALAKENANLNQV
                     RVRFTQADAFAYIRDMAAGGRQFDVVVLDPPKLVRSRAELEEGARKHFDLNRLAMRLV
                     APGGLMVTCTCAGLITSDEFLRMIYASARQAGPEVSPATADRGARHAARHVQILAKTG
                     AAADHPIATNCPEGEYLQAVWLRLL"
     gene            complement(2996..3235)
                     /locus_tag="VFB80_01200"
     CDS             complement(2996..3235)
                     /locus_tag="VFB80_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32395.1"
                     /translation="MQRRVIPFLVGAVLLVFGFFCLNYTKADTEQRHKVFAARNNLPP
                     PSRPIFYGGALSLALGAGLIGFAVGRNPAAASRAS"
     gene            3349..3957
                     /locus_tag="VFB80_01205"
     CDS             3349..3957
                     /locus_tag="VFB80_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN32396.1"
                     /translation="MTSDSAISHWMLDLAAGDADAAQRLWQRYFAQLVELAARKLPPP
                     VRRVVDGEDVALSAIDSFCKGVERGRFPRLNDRDDLWRLLVVITARKAADATQHARRQ
                     RRGGGRVRGDSALGLPERDDGPGWEQVVGAEPTPEFSCQVREELDQRLAALGDPVLKE
                     IALSKLDGHTTDEIARRLNVVPRTIERKLALIRRRWSPEESP"
     gene            3954..>5253
                     /locus_tag="VFB80_01210"
     CDS             3954..>5253
                     /locus_tag="VFB80_01210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32397.1"
                     /translation="MSNSAADPFTTLPLADAERLDKVCDAFEAAWRDENGEPPRLDEF
                     LTPLDAEMQSAALLELLPIELAYLKQRGRPISPSDYRRRFPTAAPYLEQWLADYPQTA
                     ARQLACGERFAKYTITAFVDAGGMGELYRANDAVLGREVAIKVLLRRYRWSPEALARI
                     STEARALAALNHPHIVTVHELAEHEGVAYIAMEFLEGQTLADRLEEGPLPPDEALGIA
                     SALARALATAHEKQIIHRDLKPKNIFLTAAGPTKVLDFGLARLAEMQAEPAGSDTSPV
                     AASGNGTATGSRLGTTGYMSPEQIHGQSVDARTDIFSFGCVLFEMLLGSPAFKRGSSA
                     ETDEATLHAPLPQPASAAGGLAESLYGVCERCLRKSPAERYASALQLTQELAEIETSH
                     AQRRGRTLRHIEHSLLAAACILLVGLGIWWWQRSAEQARLT"
BASE COUNT          941 a         1719 c         1729 g          864 t
ORIGIN      
        1 ggccagcacc gcttgaccga ctttctccgc cgtcggctcg ccgcgaatcg cgcccggcga
       61 tggctgctca gcgctggcgc aggccgcgcc ggcggccagc agcgccgcgg cgtgttcgtg
      121 gaactgccgc cggctgatcc aaccaagggt cccgctcatc gaaattctcc tttcgcccat
      181 tggcagagcg tattgcattt gcaaaacgct cgccacaagg cattaccgcg gcaggctttg
      241 cgccctccgc ggagcccctg gcgcgcgaag tttccgggaa aatcggccat agcagctggt
      301 gccaccacca ggaatgattg acttcgcgcg gcccatcctt aaaatcggcc tgaccggcaa
      361 aaccccagcg gtttgcctac ttgcctctgc tagcccgcct ttccttagta gcgattctgc
      421 gtgaacaaaa aggagcggcg aggggcgaac caggtcctgc ccgcctcgta caagttgaac
      481 ccgcggagtc gcctcgtggc gaattcccgc ttcgctgttg tcccccatcg ttctgaatat
      541 cggaggaacc ccatgctgcg tcgtgttttt ttggccctga cggtcgtggc gctgtgcggc
      601 ccggcttatg ccgccgaagc ggaggtaggc gcgaaggccc cggacctgaa gggcctgatc
      661 ggaacggacg gcaaggaata caacctggcc gacatgaagg acaagatcgt cgtcgtttgc
      721 ttcacctgca acgcctgccc ggtggccgtg ggttatgaag accgcttcat cgagttccag
      781 aagaagtacg gcagcaaggg cgtgaagttc atcgcgctga acgccaacaa gaagaccgaa
      841 gacatcgccg tgatgaaggc ccgggccgaa gagaagggct tcaacttccc ctacgtctat
      901 gacaagaccg gcgagtcggc cacggcctac ggcgctcgcg tgacgccgca catcttcgtg
      961 ctcgaccagg accgcaaggt cgcctacgtc ggctcgttcg acgactccca gaacaacccg
     1021 aagaagcagt atgtctcgga cgccgtcgat gccctgctcg ccggcaaaaa ggtcgagttc
     1081 accaacagca aggcgtttgg ctgcggcatc cagaacaagt agccgccccg tctgccttga
     1141 aaaattgccc gccgcctccg cgctagtgcg ccgaggcggc gtttttgttc ccgccttcac
     1201 cccggggatt cgccatgcgt ttttggatta gattcgcggt cctcggcctc gccagcgcgc
     1261 tggccggttg cgactacaac gcgccgctgg gctcatcgcc cacgcctgtt gcgagcgcta
     1321 gcgagattca actgccacaa ggcgacgcca aggcgctgca agccatcatc gacaagcaca
     1381 agggcgaagt ggtcttcgtc gatttctggg cgacctggtg cgggccgtgc gtcgagggct
     1441 ttccgcacac ggtcgagctg agcaagaagt acaaggggca agggctggcg acgatcgccg
     1501 tcagcttcga ttcgctcgag aatgaaggtg cggttcgcga attcctgaag aagcaaggcg
     1561 ctgatttcga ccatctgctc agcaagtacg acggcgtgag ccaggatgcc gcggtcgatt
     1621 ttgatgtcga ggccctgccg caataccggc tctacgaccg gcaaggcaag ctgcggcaca
     1681 agtgggaagg caagtcggaa gagatcgagc cagcgatcca gacgctcttg gccgagaaat
     1741 agttacagca gccgcaacca caccgcttgc aggtactcgc cctcgggaca attggtggcg
     1801 atcggatggt cggccgcagc gccggtcttc gccaggattt gcacgtgccg cgcggcgtgg
     1861 cgtgcgccgc ggtcggcggt ggcgggcgag acctcggggc cggcctgccg ggcggaagcg
     1921 tagatcatcc gcaggaactc gtccgaggtg atgagtccgg cacaggtgca cgtcaccatc
     1981 aggccgcccg gcgcgaccag ccgcatggcg aggcgattca aatcaaagtg cttgcgtgcg
     2041 ccttcctcga gttcggcccg cgagcggacc agcttaggtg gatccagcac gacgacgtcg
     2101 aattgccggc cgccggccgc catgtctcgg atgtacgcaa aggcgtcggc ctgcgtgaat
     2161 cgcacgcgga cctggttgag attggcgttt tcctttgcca gtgccagggg ctcggcgtcc
     2221 aaatcgacgc cgatcgcctc tttcgctccg cccagcctca tggcctggat cgcgaagccg
     2281 ccggtatagc agcacaagtc gagcaccgtt ttgcccccgc aaaagctcgc cagaagcttg
     2341 cggttgtcgc gctggtcgca aaagaatccc gtcttgtgcc cgcttgaaaa atcgacgcgg
     2401 aaccgcgtgc cgaattcctg gatcgtgacc cgcgagggca gctgcggcga cgcgaccgcc
     2461 tcgccctcaa aaccctcctg ggccagcgac gccggcccgc cgcgaatcag agtgtgctgc
     2521 gtacccaaca gcggcgcaag cagcgcaaga atcgcctccg cccgctggta catgcccacc
     2581 gaaaaacact cggccgaaag aacgttgcac agcttgtcga tcacgatgcc ggagaggccg
     2641 tcgccttcgg catgcaccac gcggtaggcg tcggtcacct cgttcagccg cagtgtttcg
     2701 tgccgcaacc ggacagcgtc ggccaggcgg cgctgccacc aggcttcgtc cggcggctcg
     2761 cccaggctga gcatccgcag cgacagttcg gacttgggat tgaatagccc atagccggtg
     2821 ggctggcccg cagcgtcctg tacctggacc aggtcgcccg gcgctgcttc gcgatcgacg
     2881 cgggccaggc gcttccggta gatcagcggg tgcaggccca cggacttcag ctgcacggcg
     2941 ggaagctgcg gcggcatcgc cagcgagcgc gtcgtcgaat tcagcggttg aggcattacg
     3001 aagcgcggct ggcggctgct ggattccggc cgacggcgaa gccgatcagc cccgcgccca
     3061 gcgccaggga gagcgcgccg ccatagaaga tcggccggct cggcggcggc aggttgtttc
     3121 gggcggcgaa cactttgtgc cgctgctccg tgtcggcctt cgtgtagttg aggcagaaga
     3181 agccgaacac gaggagcacg gcccccacca ggaatggtat cacgcggcgc tgcatgcctt
     3241 ggattctagc cgcgagcggc cggctgcctc tagaccggca aattccattg gcccgcagcg
     3301 ggcgctgcgg ctagaatggc cctcggaaaa gtacgcaagt gcaagtggat gacttctgac
     3361 tcagcaatct cccactggat gctggacctg gccgcgggcg acgccgacgc ggcgcagcgg
     3421 ctgtggcagc gctacttcgc ccagctggtc gagctcgccg cccgcaagtt gccgccgccc
     3481 gtccgccgcg ttgtcgatgg cgaggatgta gcgctgagtg cgatcgacag cttctgcaag
     3541 ggcgtcgagc gcgggcggtt tccgcggctg aatgaccgcg acgacctgtg gcggctgctg
     3601 gtcgtaatca ccgcccgcaa agccgcggat gccacgcagc acgcccggcg gcagcggcgc
     3661 ggcggcgggc gggtgcgggg agattcagcg ctgggcctgc ccgagcgcga cgacggcccc
     3721 ggctgggagc aggtcgtcgg cgcagagccg acgcccgaat tcagttgcca ggtgcgcgaa
     3781 gagctcgacc aacggctggc cgcgctgggc gatccggtcc tgaaggagat tgccctgtcc
     3841 aagctcgacg ggcatacgac cgacgagatc gcccgccggc tgaacgtcgt tccgcggacg
     3901 atcgagcgca aattggccct catccgccgc cgctggtcgc cggaggaatc cccatgagca
     3961 attccgctgc ggatcccttc accacgctgc cgctggccga tgccgagcgc ctggacaagg
     4021 tctgcgacgc gtttgaggcg gcctggcgcg acgaaaacgg cgagccaccg cggctggatg
     4081 agtttctgac gccgctggac gcggaaatgc aatcggccgc gctgctcgag ctgctgccga
     4141 tcgagctggc ctatctgaag cagcgcggga ggccgatctc gcccagcgat taccgccgtc
     4201 gatttcccac ggctgccccg tacttggagc aatggctcgc ggactatccg cagacggccg
     4261 cgcggcaatt ggcctgcggc gagcggtttg ccaaatacac gattacggct tttgtggacg
     4321 ccggcggcat gggcgaactc tatcgcgcga acgacgcggt gctcggccgc gaagtggcga
     4381 tcaaggtatt gctgcgccgc taccgctgga gtccggaggc cctggcccgc atcagcaccg
     4441 aggcccgcgc actggccgcg ctgaaccacc cgcacatcgt gacggtccac gaactggccg
     4501 agcatgaagg ggtggcctac atcgcgatgg aattcctcga gggccagacc ttggcagacc
     4561 ggctggagga gggaccgctg ccgccggacg aggcgctcgg cattgcctcc gcgctggccc
     4621 gggccctggc gaccgcacat gagaagcaga tcatccaccg cgatctgaag ccgaagaaca
     4681 tctttctcac cgcggccggg ccgacgaagg ttctcgattt cggcctggcc cgactggcgg
     4741 agatgcaggc ggagcccgct ggttcggaca catcgcccgt cgccgcttcg ggaaacggga
     4801 cagcgaccgg atcgcgcttg ggcacgacgg gctacatgtc gcctgagcag atccacgggc
     4861 agagcgtgga cgcgcggacc gacatattct cgttcggctg cgtgctcttt gaaatgctgt
     4921 taggcagccc cgcgttcaag cgtggctcgt ccgcggagac cgacgaagcg acgcttcacg
     4981 caccgctccc ccaaccggct agcgccgccg gcggcctggc ggagtcgctg tacggcgtat
     5041 gcgagcgttg cctgaggaag tcgcccgcgg agcggtatgc cagtgccctg cagctcactc
     5101 aagaactggc cgaaatcgag acgtcacatg cccagcgtcg cggacggacg ctgcggcaca
     5161 tcgagcattc actgcttgcg gccgcgtgca ttctgctcgt cggattgggg atctggtggt
     5221 ggcagcgcag cgccgagcaa gcacggctta ccg
//
LOCUS       DASRJB010000043        14291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1990046, whole genome shotgun sequence.
ACCESSION   DASRJB010000043 DASRJB010000000
VERSION     DASRJB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990046"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFB80_01215"
     CDS             complement(<1..1185)
                     /locus_tag="VFB80_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32398.1"
                     /translation="MQRLLMLAEASATIQYKLARLQTLTEWWHWMVLLTLALAIGAFV
                     VWMYRRDSVELPRSLAITLCLLRLVAFVGILFFFFGLEKRAERNLVKNSRAILLIDTS
                     QSMGLRDSDSSNVPAAMMRVEHVAAELAGGAFIEKLREKHDVIAYRFDQGDNPIEIAT
                     YPRKATKAETAEAQISEEDRLRQIASQSRWLAYVAAGILVASIVAGLVYLIGGRKWST
                     RSASGQTARGARGLQKEEPTSWALLVCMTLLIAAAVVMATASLRGSEAGLLAILGIEE
                     PKPSAAAAAPEKKAEQVVPQVDWTEQLKPRGALTRIGDNLKYLIDKERGGPIAAVLLF
                     TDGGNNAGSDVKLAATAAGDATIPIYTVGLGSDKRPANVRVVDLEAPERVYPGDKFTL
                     TGY"
     gene            complement(1185..3503)
                     /locus_tag="VFB80_01220"
     CDS             complement(1185..3503)
                     /locus_tag="VFB80_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN32399.1"
                     /translation="MQFVHQALTWGFLLALVPLLIHLINMMRHRRVQWAAMEFLLASY
                     KKHRKWVWLKQLLLLLARMAAVALIVAMLAQLKTRDQWLAIFGGRVTHHYVLLDDSYS
                     MQDRVAGASAFDAAKQVMQAIVGRATQQDSPQKLTLLRYSQARGSATDGVAEQVADFN
                     AEPIDSKFDVTLERKIRVLEPTQLASGPLEALGMLRQLLTGAKDETSIVYVLSDFRTK
                     DWGSPTELRDLLGQLRRSQAEIHLVNCARSSEPNLGILSIEPADETRAAGVHLFVNVE
                     VKNFGAKPATKVQLKLQSTSYSSDNLAATPPDELKGQPDDLGTLLIDQIEPGEKITRR
                     VQVYFPQAGKHVVEATLPEDPVAADNHRWCVIDFPDGEHVLIVDGSDSQQQQYYLQVA
                     FQPLARSNTGIRTETKPAAFLRDATPEALNAYSAIYLLDVPRLDGKAAETLDAYVRGG
                     GGLAIFTGAGINAAYYNQTLYRDGQGLLPAPLGPDVELPPALDPSEPDLELTNHPIFA
                     FFMGETNTLIRGVKIDRYRKLADGWKPGPAQAVEIIARTRDRAPLAVEKKHGQGEVVM
                     YLTTLAPEWNDWAKNPSFVVVALKTQSYLATAHRLDDPRLVGTPLVLELPAADYRSDV
                     AFVAPGEQPGSRTKIDRQFSAQESPATTATASLGQTVVEGKPRGETDRAGIYEAWPIT
                     TKGDIDVRRWAFNVDPAEGDLAAVTAADLTNSLDPVKVNYHLADQYQQEDVASTGYNL
                     STLILFGLILLLIGEQVLAYSASYHVQPGAVR"
     gene            complement(3583..4485)
                     /locus_tag="VFB80_01225"
     CDS             complement(3583..4485)
                     /locus_tag="VFB80_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN32400.1"
                     /translation="MPGSKRFLHPEAIKRISRLEIRARHIVEGFLSGMHKSPYFGQSI
                     EFLQHREYVTGDDLRHVDWKVWARQDRLYVKQFEEETNLRCHLLVDLSRSMAYGNGPL
                     NKYEYACTLACSLAYLVLRQQDGVGCTTFDERIRVTVPVRTMHNHIFSIVDSLAVQEP
                     QDKTDMHAILRTVAESAPRRGVMVLVSDLLADRPGLFKGLRLLRQRGHDVLIFHVMDD
                     EELDFPFNGPTRFEGLEIPDYLNCNPRALREGYLAALQEYLDDIRHQAARSSCDYALV
                     RTSEPLDAALATFLSKRLAVMNRY"
     gene            complement(4454..5530)
                     /locus_tag="VFB80_01230"
     CDS             complement(4454..5530)
                     /locus_tag="VFB80_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN32401.1"
                     /translation="MTDGRREGDVVQATIAESAAIRRLNDAREKIVAQLSQVIVGQAD
                     VIEELLICLFARGHCMLEGVPGLAKTLMISTLARTLNLSFSRVQFTPDLMPADITGTE
                     ILEENRSTGSRERRFLEGPLFSNIILADEINRTPPKTQAALLEAMQERQVTVGRVRHP
                     LMDPFFVLATQNPIEQEGTYPLPEAQQDRFMFKVFVNYPNFNEEFEVARRTTALQTDT
                     IQAVLSGQEIIDLQRTVREVPVSDHIIRYALSLVRQTRVNQTGVPDFVNDQVGWGAGP
                     RAVQYLILGGKARALLRGRTHVSTDDIQALAKPVLRHRLVVNFAAQSDGVTADKIIDR
                     LIASTPTKEDELTSDARFKKIFAS"
     gene            5923..6741
                     /locus_tag="VFB80_01235"
     CDS             5923..6741
                     /locus_tag="VFB80_01235"
                     /inference="COORDINATES: protein motif:HMM:NF042726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HZN32402.1"
                     /translation="MRQQICGGLMALVLIGATGCGINKSRLATEQIVISDAVDRAVAS
                     IDFSPLSGRKVYFDTQYLDGVNMGPNGNIKYVVSSLRQQMLAYDCRLQEKAETAEYIV
                     EARVGALANDGHEVTYGVPGGAAMASASVLFSSPLTAPAMPELSLGRRNHHAGTAKLG
                     LFAYDRATREPVWQAGVTKATSEARDAWVLGLGPYQKRAAAPRGGLWWKKPEPRAPLD
                     PLAAYSRSATFQRALAAPAGSAPQPEIATASHLQPPAPIALPPPDKVLPLPQMP"
     gene            7041..8453
                     /locus_tag="VFB80_01240"
     CDS             7041..8453
                     /locus_tag="VFB80_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HZN32403.1"
                     /translation="MRNARLIWQAALAVAILAAAVEDSARGQNNNNNANNNNGGNVNV
                     GIIGNLPAGVVISPDGVLRVKQSPDPNGQLTKTRIAQARARLGNVAKVSELRKVSLNR
                     LEAALAAQLAAGKEPTDEMKYLAGLTRAQYVFYYPDSKDIVLAGPAEGFFLDPSGRVL
                     GMNSGRAVLELQDLVAALRAFPPEGKQAHVISVSIDPTQEGLARMAAWLKSIRPGPGD
                     AARIVAGLKENLGLQNVTVEGISPRTHFAQVMVEADYRMKLIGIGIEPPPVKMATYID
                     KATQNESLQRWFFTPNYDCVRVAEDSLAMELVGQGVKLIGESELVAADGARRTTGGNQ
                     SRASKAYCDAFTANYPQIAARNAVYAQLKNLIDLSIAAAFIQKQDYYGQATWKLETFG
                     DERKYAVETFEAPKTVETACTAVWKGNRLMTPVGGGVQIQPLEAMATSNLLSDDKGAV
                     KQAKAKTTLSGLANGQWWWD"
     gene            complement(8825..10585)
                     /locus_tag="VFB80_01245"
     CDS             complement(8825..10585)
                     /locus_tag="VFB80_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZN32404.1"
                     /translation="MNLLYIVLPSAAILLVAYFTYGKLLARLFQLNPQRKTPAYEMQD
                     GVDYEPLTSSALFPQHFSAIAAAGPIVGPILAGLTFGWLPALLWILIGSILIGGVHDF
                     GALVASIRHKARSIAEVVRENMSRRSYLLFLSFIWIALIYIIVAFTDITASAFVGPPT
                     ADSGGAGGGAVATSSLLYLVLPIVMGLLMRYGKLSLTWATVIFVPLVGVAIWVGKYIP
                     LDLQSLLGFSSDKIGEANARKVWDVLLLLYCLVAGVVPVWMLLQPRGHLGGFFLYAAL
                     GAGAIGLAIGGATAQYPAFTGLAVPKADGSLEMLFPMLFITVACGACSGFHSLIASGT
                     TSKQLKVETDARTIGYGAMLLEGMVAVVSLCCVMMFAAGSEQLRGTPNQIYAQGIGRF
                     LGVIGVERGFGIAFALMAFTTFVYDTLDVCTRLGRYILQELTGWRGNFGRWFATGLTA
                     GVPVFFLLRHPSDAAVPVWRIFWSLFGASNQLLAALTLLGVTIWLWRTRGAWWVWLVT
                     GVPTAFMYVMSTWALLAMTLPKFWTQTGWTMPADPVPWIGLVLIVLAALMLIEAIRIL
                     LALGSPPPAGRLEAAAAGAS"
     gene            10738..13518
                     /gene="uvrA"
                     /locus_tag="VFB80_01250"
     CDS             10738..13518
                     /gene="uvrA"
                     /locus_tag="VFB80_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN32405.1"
                     /translation="MSSKCIELRGVAVHNLKQVDLDIPHRKLVVLCGVSGSGKTSLAL
                     DTLYAEGQRRYIESFSLYSRQFLQRLDKPAAERIDGLPAAVAVTRHEAGRSSRATIGT
                     TTETLDYLRLLFAKIGRLICPTCSRPVERTSPEAAARQLGQLPEGRRLMVGFAAEQAA
                     GESREQHVARLNQAGFVRLAEADSGLLVVVDRLVAGQAAEQRLRDSLETAYQHGGGRA
                     LGLVEADPNAPDAVTIDDRPWQRLSFSSRLECPVCRRGFADAEPRRLSFSSPLGACPT
                     CEGFGNVVELDMKLIVPDPEKSIREGAIAPWNTPAYKHELEELLALAPDYGIPVHAPF
                     RELTAEHVRLIREGVPERNFGGLAGFFRWLERRKYKMHLRVFLNRWRTAKLCPTCRGS
                     RLNEEALAWRIGGKNIAELTQLKIDDARTFLAGLPLDEFEGRASRQLLSQVDSRLGFL
                     QQAGLGYLALDRTLKTLSGGEAQRAVLTSALGSSLVNMLYVLDEPSVGLHPRDVDRLV
                     AAIRGLCDRGNSVVVVEHEESLLRAADELIEIGPGAGDRGGEVAFQGTLDEMLQENAS
                     LTGEYLAGRRGISVPQRRRATNRGWIKLLGARGHNLRGETFEFPLNTLCLVTGVSGSG
                     KSTLVQDTLYGALCRRQRKECDEPLPYGDVVGCGQIDDVILVDQSPIGRSPRSNPVTY
                     IKAFDPIRAVFAEAVEARTHNYTAGHFSFNVEGGRCPACEGEGYTKVDMQFLADIYRR
                     CGQCGGTRYRKEILAVKYRGQNIADVLGMTVREAFGFFRGQAKVQARLKQLIDVGLDY
                     LQLGQPANTLSAGEAQRLKLAAHLSEASRKRTLFLLDEPTTGLHFADVVTLLDCFEAL
                     LSVGHSLIVVEHNLQLMRAADYLIDLGPGAGEDGGRIVAKGTPEEVAANLASHTGRFL
                     AQSLLIGQSSLD"
     gene            13511..>14291
                     /locus_tag="VFB80_01255"
     CDS             13511..>14291
                     /locus_tag="VFB80_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32406.1"
                     /translation="MTDLRELDVFFGDLTVNRAFVYARMPLPAEAGDWSLSGQVRGPR
                     CLTAQTLPATSLLEDLGPGPTLMARAAVPDPCFWSPDLPAIYDLTVNLRRGGQIVATG
                     SREVGLRALGVRGRHLALESKRWVLRGVCPESTHAALPRQWHEALACYVAVDPRVEVL
                     AEASQWGALAVVEVRGEAGSLLALLRELAKFPAVALAVVHGELPAEFKPSQAAPNLLL
                     AQAIGDGDELAVRPWTNVLWAETDDSLRIGRISTLAELPVVA"
BASE COUNT         2498 a         4712 c         4638 g         2443 t
ORIGIN      
        1 gtagcccgtg agcgtgaact tgtcgcccgg atagacccgc tcgggcgctt cgagatcgac
       61 gacacgcacg ttggccggcc gcttgtccga tcccaggccg acggtgtaga tcgggatcgt
      121 ggcgtcgccg gcggcggtcg ccgccagctt cacgtcgctg ccggcgttgt tgccgccatc
      181 ggtgaagagc agcacggcgg cgatcggccc gccccgttcc ttatcgatca ggtatttgag
      241 gttgtcgccg atccgcgtca gcgctccgcg gggcttgagc tgttccgtcc aatcgacctg
      301 cggcacgact tgctcagctt tcttctccgg cgctgcggcc gcggcgcttg gcttgggctc
      361 ttcgatgccg agaatcgcca gcaggcccgc ctcggagccg cgcaggctcg cggtcgccat
      421 caccaccgcc gccgcgatca agagcgtcat gcacaccaaa agcgcccagc tcgtcggttc
      481 ctccttctgc agtcctctgg ctccgcgagc ggtctgtcca ctcgcggagc gagtggacca
      541 ctttctcccg ccgatcaaat acaccaggcc cgcaacgatc gacgccacca gaatccccgc
      601 cgcgacataa gccagccatc gcgactggct cgcgatctgc cgcaggcggt cttcctcgga
      661 aatctgcgcc tcggccgtct cggccttcgt ggccttgcgc gggtacgtgg cgatttcgat
      721 cgggttgtcc ccctggtcga agcggtaagc gatcacgtcg tgcttttcgc ggagcttctc
      781 gatgaacgcg ccgccggcca gttccgccgc cacgtgttcc acccgcatca tcgccgcggg
      841 cacgttgctc gaatcgctgt cgcgcaggcc catgctctgg ctggtatcga tgagcaggat
      901 cgcccgcgaa ttcttcacca ggttccgctc ggcccgcttc tccagcccga agaagaagaa
      961 caggatgccc acgaacgcca ccagccgcag caggcaaagc gtaatcgcca agctccgcgg
     1021 cagctcgacg ctgtcgcggc ggtacatcca gaccacgaac gccccgatcg ccagcgccaa
     1081 ggtcagcagc accatccagt gccaccactc ggtgagcgtt tgcagccggg ccagcttgta
     1141 ttggatggtg gcggacgcct ccgccagcat cagcagtctc tgcatcagcg gacggctccg
     1201 ggttgcacgt ggtagctcgc ggaataggcc agcacctgct cgccgatcaa gagcaggatc
     1261 aagccgaaca gaatcagcgt gctgaggttg tagccggtcg aagccacgtc ctcctgctga
     1321 tactggtcgg ccaggtgata gttgaccttc acggggtcga ggctgttggt cagatcggcc
     1381 gcggtaacag cggccaggtc cccttcggcc ggatcgacgt tgaaggccca ccgccgcacg
     1441 tcgatgtcgc ccttggtcgt gatcggccag gcttcgtaaa tgcccgcgcg gtccgtctcg
     1501 ccgcggggct tgccttcgac gaccgtctgg cccagcgagg ccgtagccgt cgtggcggga
     1561 gactcctggg cggagaattg ccggtcgatc ttcgtccgcg agcccggttg ttcgcccggc
     1621 gcgacaaagg ccacgtcgct gcggtagtcg gctgcgggca gctcgagcac cagcggcgtt
     1681 cccaccagcc gcggatcgtc caggcggtgc gcggtggcca ggtacgactg cgtcttgagc
     1741 gccaccacga cgaagctcgg attcttggcc cagtcgttcc actcgggcgc gagcgtcgtc
     1801 aagtacatca cgacttctcc ctggccgtgc ttcttctcga cggccaaggg cgcgcggtcg
     1861 cgggtgcggg cgatgatctc gaccgcctgc gccgggccag gcttccagcc gtcagccagc
     1921 ttgcggtagc ggtcgatctt cacgccgcgg atgagcgtgt tggtctcgcc cataaagaat
     1981 gcgaagatcg gatggttcgt caattccaag tcgggctcgc tcggatcgag cgccggcggc
     2041 agttccacgt ccggtcccag cggcgcgggc aacaggccct gcccgtcgcg atagagcgtc
     2101 tggttgtagt acgccgcgtt gatccccgcg ccggtgaaga tcgccaggcc gccgccgccg
     2161 cgaacgtacg catcgagcgt ttccgccgcc ttcccgtcca gccgcggcac gtcgagcagg
     2221 taaatcgctg aataggcgtt gagcgcttcc ggcgtggcgt ctcgaaggaa cgccgccggc
     2281 ttggtctccg tccggatgcc cgtattcgag cgggccagcg gctgaaaggc gacctgcaga
     2341 taatactgct gctgctgcga atcggagccg tccacgatca gcacgtgctc gccgtcgggg
     2401 aagtcgatca cgcaccagcg gtggttgtcg gcggcgacgg ggtcttccgg cagcgtggcc
     2461 tcgacgacgt gcttgccggc ctgcgggaaa tagacctgca cgcggcgggt gatcttctcg
     2521 ccgggctcga tctggtcgat cagcagcgtc cccaggtcgt cgggctgccc cttcaattcg
     2581 tcgggcggcg tcgcggcgag gttgtcgctc gaatacgagg tcgattgcag cttgagctgg
     2641 accttcgtcg cgggcttcgc gccaaagttc ttgacctcga cattgacgaa caggtgtacg
     2701 ccggcggccc gcgtctcgtc ggccggctcg atcgagagga tgcccaggtt cggctcgctg
     2761 gagcgggcgc agttcacgag gtggatctcg gcctgcgacc gtcgcagctg gcccagcagg
     2821 tcgcgcagct cggtcgggct gccccagtcc ttggtgcgga aatccgacag cacatagacg
     2881 atactggttt cgtctttggc ccccgtgagc agctgccgca gcatgccgag ggcctcgagc
     2941 ggtccggacg ccagctgcgt cggctccagg acgcggattt tccgctccag cgtcacgtcg
     3001 aacttcgaat cgatcggctc cgcgttgaag tcggcgacct gctcggccac cccatcagtg
     3061 gccgagccgc gagcctgcga atagcgcagc agcgtcagct tctgcgggct gtcctgttgg
     3121 gtggcccggc cgacgatcgc ctgcatgacc tgcttggccg cgtcgaaggc gctcgcgccg
     3181 gccacgcggt cctgcatcga atagctgtcg tccaaaagca cgtaatggtg cgtcacgcgg
     3241 ccgccgaaga tcgccagcca ctggtcgcgc gtcttcagct gcgcgagcat cgccacgatt
     3301 agcgccaccg ccgccatccg tgccagcagc agcaacagct gcttcagcca gacccacttg
     3361 cggtgcttct tgtagctggc cagcaagaat tccatggcgg cccactgcac gcgccgatgc
     3421 cgcatcatgt tgatcaggtg gatcaacagc ggcacgaggg cgagcaggaa gccccaggtc
     3481 agtgcttgat ggacaaactg cattttatgt catttgtcat ttgtcacttg tcacttgtca
     3541 tttgagctct cctgcaaatg actactgacg agtgacaaat gactagtagc ggttcatcac
     3601 cgcaagtcgc ttgctaagga acgtcgccag cgccgcatcc agcggctcgc tcgtccgcac
     3661 cagcgcgtaa tcgcaactgc tgcgggcggc ctggtggcgg atgtcgtcga ggtactcttg
     3721 cagtgcggcg aggtagccct cgcggagcgc ccgcgggttg caattcaaat agtcgggaat
     3781 ctccaacccc tcgaaccgcg tcggcccatt gaacggaaag tccagctcct cgtcgtccat
     3841 gacgtggaag atcagtacat cgtggccccg ctgccgcagc agacgcaggc ccttgaacag
     3901 cccgggccga tcggccagca aatccgagac gagcaccatc acgccccgcc gcggcgcact
     3961 ttccgccacg gtccgcagga tggcgtgcat gtcggtcttg tcctgcggct cctgcaccgc
     4021 cagcgaatcg acgatgctga atatgtggtt gtgcatcgtg cggacgggta cggtgacgcg
     4081 gatccgctcg tcgaacgtcg tgcagccgac gccgtcctgc tgccgcagca ccaggtaggc
     4141 cagcgagcag gccagcgtgc aggcgtactc gtacttgttg agcgggccgt tgccgtaggc
     4201 catgcttcgc gacagatcga cgagcaggtg gcagcggagg ttggtctctt cctcgaactg
     4261 cttgacgtac agccggtcct gccgggccca gactttccag tccacgtgcc gcagatcgtc
     4321 gccggtgacg tattcgcgat gctgcagaaa ctcgatcgac tgcccgaaat acgggctctt
     4381 gtgcatcccc gaaagaaacc cttcgacgat gtgccgggcc cggatttcca gccgcgagat
     4441 ccgcttgatc gcctcaggat gcaaaaatct ttttgaaccg ggcatcgctg gtgagctcgt
     4501 cttccttggt gggagtgctg gcgatcaggc ggtcgatgat cttgtcggcg gtcacgccgt
     4561 cgctctgggc ggcgaagttg accaccaggc ggtgccgcag caccggcttg gccagggcct
     4621 ggatgtcgtc ggtgctgacg tgcgtgcggc cgcgcaacag ggcccgcgcc ttgccgccca
     4681 ggatcagata ctgcacggcg cggggaccgg cgccccagcc gacctggtcg ttcacaaaat
     4741 cgggcacgcc cgtctggttg acgcgcgtct gccgcaccag cgacagggcg tagcggatga
     4801 tgtggtcgct gaccggcacc tcgcgaaccg tccgttgcag gtcgatgatc tcctgcccgc
     4861 tcagcaccgc ttgaatggtg tcggtttgca gcgcggtcgt gcggcgggcc acctcgaact
     4921 cctcattgaa gttcgggtag ttcacgaaga ccttgaacat gaagcggtcc tgctgcgctt
     4981 cgggcagcgg gtaggtccct tcctgctcga tcgggttctg cgttgcgagc acaaagaacg
     5041 ggtccatcag cgggtgccgc acgcggccca ccgtcacttg ccgctcctgc atcgcttcca
     5101 gcagcgccgc ctgcgtcttg ggcggcgtgc ggttgatctc gtccgccagg atgatgttcg
     5161 agaacagcgg cccttccagg aaccgccgct cgcgcgagcc cgtcgagcgg ttctcttcca
     5221 ggatttccgt ccccgtgatg tccgccggca tcaggtcggg cgtaaactgc acgcggctga
     5281 acgacagatt cagcgtgcgg gccagcgtgc tgatcatcag cgtcttcgcc aggccgggca
     5341 cgccttccag catgcagtgg ccgcgggcga acaggcagat cagcagttcc tcgatgacgt
     5401 cggcctggcc gacgatcacc tgcgaaagtt gggcgacgat cttctcccgg gcgtcgttca
     5461 ggcggcgaat tgcggctgat tccgcaatcg tcgcttgcac cacgtcgccc tcgcggcggc
     5521 cgtcagtcat acaaggcctt tcgttggttt ttggtgcagg tttacagcgc agcggaagcg
     5581 taaaccattg cgttctaata gtttacgtta cgcggggcga attgtggcgc tctccggcga
     5641 ttcggcgggc aggtcctaag tccaaggccg gcttagggtt ggtgcatcca aggggcaggt
     5701 tggttcaaaa gccgcccgcg gccgcaccac aggccgcgag cgggcacgca ctaacgatag
     5761 tcgcttccta acattttcct agcagggagg ttgtgacgat tgcaaagatt ttgcggcccg
     5821 aaaggctacc aggttactgc atttctgcta gcagattccg cgttggtgac gcggaacctg
     5881 ccggctacac tcccggccct cgaatactgg cagaattgca agatgcgaca gcagatatgc
     5941 ggcggcctga tggccctcgt tttgattggc gcgacgggct gcgggattaa caagtcgcgg
     6001 ctggcgaccg agcagatcgt catttccgac gcagtcgacc gggcggtcgc ctcgatcgat
     6061 ttcagcccgc tgtcggggcg caaggtctat ttcgatacgc agtacctcga cggcgtgaac
     6121 atggggccga acggcaacat caagtacgtc gtcagctcgc tgcggcagca gatgctggcc
     6181 tacgattgcc gcctgcaaga gaaggccgaa acggccgagt acatcgtcga ggcccgcgtc
     6241 ggggccttgg ccaacgacgg ccacgaagtg acctacggcg tgcccggcgg cgcggcgatg
     6301 gcttctgctt cggtgctgtt ttccagcccg ctcaccgcac cggcgatgcc ggaattgtcg
     6361 ctcggccgcc gcaaccacca cgcgggaact gccaagctgg gcctgtttgc ctatgaccgg
     6421 gcaacgcgcg agccagtctg gcaggcaggc gtcaccaagg ccactagcga agcccgcgac
     6481 gcctgggtgc tgggccttgg cccttatcaa aagcgggcgg ccgcgcctcg cggcggcctg
     6541 tggtggaaga aacccgagcc gcgagcaccg cttgatccgc tggccgccta tagccggagc
     6601 gcgacgttcc agcgggccct ggccgcaccg gccggctccg ctccgcagcc agagattgcg
     6661 acggcctccc atctccagcc tcctgctccg atcgccctgc ctcctccgga caaagtgctg
     6721 cctctgccgc aaatgcccta ggccgcctgc ctgtgtacgc gtgtttatcg ggactgccag
     6781 cgcagccaat tcccgcgtgt cgctgccagc ctccggctcc agggctaaac gacacggcaa
     6841 ttccttgcgt gagcccggca aggcaattaa catctaatag gtagcaaaaa gccgaagcag
     6901 gctgagccga gctattccgc gcagaaagcc tgcctcgtgg acgattcaac aaccgctgcc
     6961 gcgacgaccg ccccatcgcg ggacgttgcg gcagcgcatc ggacaagcgg tcaggggcga
     7021 agatagacga ggaacgagcc atgcgtaacg cacgattgat ttggcaggcc gctttggccg
     7081 tggcaatcct ggccgctgca gtggaagact ccgctcgcgg ccagaacaac aacaacaacg
     7141 ccaacaacaa caacggcggc aacgtcaacg tcggcatcat cggcaacctg ccggccggcg
     7201 tcgtcatcag cccggatggc gtgctccggg tcaagcaatc ccccgatcca aacgggcagc
     7261 tcaccaaaac gcgcatcgcc caggcccgcg cccggctggg caatgtggcc aaggtcagcg
     7321 agctccgcaa ggtctcgctc aaccgcctgg aggcggcgct ggcggctcaa ctcgccgcgg
     7381 gcaaggagcc gacggatgag atgaagtacc tcgccggcct gacgcgcgcg caatacgtct
     7441 tctactatcc ggactcgaag gacatcgtgc tggccggccc cgccgagggc ttcttcctcg
     7501 atccgtcggg ccgcgtgctg ggcatgaact cgggccgggc cgtgctggag ctgcaagacc
     7561 tggtcgcggc gctgcgggcg ttcccgccgg aaggcaaaca ggcgcacgtg atctccgtct
     7621 cgatcgatcc gacccaggaa ggcctcgccc gcatggcggc gtggcttaag tcgatccgtc
     7681 ccggcccggg tgacgccgcc cggatcgtcg ccggcctaaa ggagaacctc ggcctgcaaa
     7741 atgtcacggt cgagggcatt tcgccccgga cccacttcgc ccaggtcatg gtcgaggccg
     7801 attaccgcat gaagctgatc ggcatcggca tcgagccgcc gccggtgaag atggcgacct
     7861 acatcgacaa ggcgacgcag aacgaatcgc tccagcggtg gttctttacg cccaattacg
     7921 actgcgtccg cgtcgccgag gacagcctgg cgatggagct ggtcggccag ggcgtgaagc
     7981 tgatcggcga gagcgagttg gtggcggccg acggcgcgcg gcgcacgacc ggcggcaacc
     8041 agagccgggc cagcaaggcg tactgcgacg ccttcacggc caattatccg cagatcgccg
     8101 cccgcaacgc ggtgtatgcc cagttgaaga acctgatcga cctgtcgatc gccgcggcct
     8161 tcatccagaa gcaggactat tacggccagg cgacctggaa gctcgagacc ttcggcgacg
     8221 agcggaagta cgccgtcgag acattcgaag cccccaagac cgtcgaaacc gcctgcacgg
     8281 ccgtctggaa gggcaaccga ctgatgacac ccgtcggcgg cggcgtgcag attcagccgc
     8341 tggaggccat ggcgacctcc aacctcctca gcgacgacaa gggcgcggtg aagcaggcca
     8401 aggccaaaac cacgctgagc ggcctggcaa atggccaatg gtggtgggac tagagcacga
     8461 cgccgatctt cttctcgcaa gcggccatcg tgaacattgg tatgctattt gcagtatagt
     8521 tcccgttgcg cggcgatcct gccattttgg ccgccgcacc cagctcgatc gcatttggtt
     8581 gctcacgtcg agggcagaat cgccgccctt ggcgattcgc tgcgagggta aggataattc
     8641 cgccgccggt tctctagtgc ttcaaatcgt aagccatgcc agtccgcagg agcgcgtcgg
     8701 aagtgcttga cctgccatgt acttgcggcg agatcggcgg cgaggcaagg cgcgaaaaat
     8761 ctcggcactg cgggaattat ccttaccttt gcggacgatc gcttagggca ttgggcggcc
     8821 gcaatcagga tgcccccgcc gcggctgctt cgaggcgtcc cgctggcggc ggcgagccca
     8881 gtgccagcag aatgcggatt gcctcaatca acatcaaggc ggctaggacg atgagcacca
     8941 gaccgatcca gggcaccggg tcggccggca tcgtccagcc cgtttgcgtc cagaacttgg
     9001 gcagcgtcat cgccagcaga gcccaggtgc tcatcacgta catgaaggcc gttggcacgc
     9061 ccgtaaccag ccacacccac cacgcgcccc gcgtgcgcca cagccagatt gtcacgccga
     9121 gcagcgtgag cgccgccagc agttgattgc tcgcgccgaa caggctccag aaaatccgcc
     9181 agactggaac cgccgcatcg ctggggtggc gcaacaggaa gaacaccggc acgccggcgg
     9241 tcagcccggt ggcgaaccaa cggccgaaat tgccgcgcca gcccgtcaac tcctggagaa
     9301 tgtagcgtcc cagccgcgtg cagacgtcga gtgtgtcgta gacgaaggtc gtaaacgcca
     9361 tcaaggcgaa ggcgattccg aaaccccgct ccacgcctat gacgccgagg aaccggccga
     9421 tgccctgggc atagatctgg ttcggagtgc cccgcagctg ctccgatccg gcagcgaaca
     9481 tcatcacgca gcagagcgag acgaccgcca ccatcccctc cagcagcatc gccccatagc
     9541 cgatcgtcct ggcgtcggtt tccaccttca gttgtttcga ggtcgtcccg ctggcaatga
     9601 gcgagtggaa tcccgagcag gccccgcagg ctacggtgat gaacagcatc gggaacagca
     9661 tctccagcga gccatcggcc ttcggtacgg ccaggccggt gaacgccggg tattgggccg
     9721 tcgccccgcc gatagccaaa ccgatggccc ccgcgccgag agccgcatac aggaagaagc
     9781 cgcccaagtg gccccgcggc tgcaacagca tccagaccgg aaccacgccg gccacgaggc
     9841 aatacaacag cagcagcaca tcccacacct tccgggcgtt ggcctcgccg atcttgtcgc
     9901 tcgagaaccc gagcagcgat tgcaagtcga gcggaatgta cttacccacc cagatcgcga
     9961 ctccaacgag aggcacgaaa atgacggtag cccaggtcag cgaaagcttg ccataccgca
    10021 tcaagagccc catgacgatc ggcaggacca gatacagcag cgacgacgtg gcaactgctc
    10081 cgccgccagc gccgcccgaa tcggcggtcg gcgggcccac gaacgccgag gcagtaatgt
    10141 ccgtgaacgc gacgatgatg tagatcagcg cgatccaaat gaaggacaaa aagagcaggt
    10201 acgaccgccg gctcatgttc tcgcggacca cctcggcgat cgaacgtgcc ttgtggcgaa
    10261 tcgatgcgac gagtgcaccg aagtcgtgaa cgccgccgat gaggatcgac ccgatcaaga
    10321 tccagagcag cgccggcagc cagccgaacg tcagtcccgc gagaatcggc cccacgatcg
    10381 gccccgccgc ggcaattgcc gaaaagtgct gtggaaacag ggccgacgag gtcagcggct
    10441 cgtaatcgac gccgtcctgc atctcgtagg ccggcgtttt gcgctgcggg ttgagctgaa
    10501 acagcctggc cagcagcttg ccgtaggtga aataggcgac cagcaaaatg gccgccgagg
    10561 ggagcacgat atagagcaga ttcatggcag tgccgggggg agaaatgcgt tcgccagcga
    10621 actttagcct acgtcagacc ggccgctgcc tgcaatcgta tcccatggtc gcgccgatgc
    10681 gttgcgaact aaaatgcagg attcgacttc gtccaatctt gcgagctagc cgagcctgtg
    10741 tcgtcaaagt gcatcgagct tcgcggcgtc gcggtccaca acctcaagca ggtggacctc
    10801 gacatcccgc accgcaaact ggtcgtgctg tgcggggtca gcggcagcgg caagacgagc
    10861 ctggccctcg acacgctcta tgccgaaggg cagcgccgct acatcgagag cttctcgctc
    10921 tacagccggc agttcctcca gcgcctcgac aagccggcgg ccgagcggat cgatggcctg
    10981 cccgccgccg tcgccgtcac caggcacgag gccggccgct ccagccgggc cacgatcggc
    11041 acgacgaccg agacgctcga ctacctgcgg ctgctctttg ccaagatcgg ccggctgatc
    11101 tgcccaacct gcagccgacc cgtcgagcgg accagcccgg aggcggcagc gcggcaattg
    11161 ggacagctgc ccgagggccg ccgcctgatg gtcggattcg ctgccgagca agcggccggc
    11221 gagagccgcg agcagcacgt cgcccggttg aatcaggcag gattcgtgcg cctggctgag
    11281 gccgacagcg gtttactggt cgtcgtcgat cggctggtcg ccgggcaagc cgccgagcag
    11341 cggctgcggg actcgctgga aacggcctac cagcacggcg gcgggcgagc cctgggcctg
    11401 gttgaggcgg atccaaatgc accggacgcg gtcacgattg acgatcgccc ttggcagcgg
    11461 ctgagtttca gcagccggct ggaatgcccg gtgtgccggc ggggattcgc ggacgccgag
    11521 ccgcggcggt tgagcttcag cagcccgctg ggggcgtgcc cgacctgcga aggctttggc
    11581 aatgtcgtcg agctcgatat gaagctgatc gttcccgatc cggagaagtc gattcgcgaa
    11641 ggggcgatcg cgccctggaa cactccggcc tacaagcacg agctcgagga gctgctggcg
    11701 cttgcgcccg actatggaat cccggtccac gcgccatttc gcgagctgac cgcggagcac
    11761 gtccggctga tccgcgaagg agttcccgag cggaatttcg gcggcctggc cggtttcttc
    11821 cgctggctcg agcggcggaa atacaaaatg cacctccgcg tgtttctgaa tcgctggcgc
    11881 accgcgaagc tctgcccgac ttgccgcggg tcgcgcctca acgaagaggc cctggcctgg
    11941 cggatcggcg gcaagaacat cgcggagcta acgcagctca agatcgatga cgcccgcacg
    12001 ttccttgcgg gcttgccgct ggacgaattc gagggccggg ccagccggca gctgctgtcg
    12061 caggtcgatt cgcggctggg cttcttgcag caagcgggcc tcggctatct ggcactcgat
    12121 cgcacgctga agaccctgag cggcggcgag gcccagcggg ccgtgctcac ctccgccctc
    12181 ggcagcagcc tggtgaacat gctctacgtt ctcgacgagc cctcggtcgg gctgcacccc
    12241 cgggacgtcg atcgcctggt cgccgctatc cgcgggctct gtgaccgcgg caacagcgtg
    12301 gtcgtcgtgg agcacgaaga gtcgctgctg cgcgcggcgg atgagttgat cgagatcggc
    12361 cccggagcgg gcgaccgcgg cggcgaagtc gcctttcagg gaaccttgga cgagatgctt
    12421 caggagaacg ccagcctgac gggtgagtat ctcgccggcc gccgcggcat ctcggtcccg
    12481 cagcgccggc gcgccacgaa tcgcggctgg atcaagctgc ttggggcgcg gggccacaac
    12541 ctgcgcggcg agacattcga gtttccgctg aatacgttgt gcctggtgac gggcgtcagc
    12601 ggctcgggca agagcacgct cgttcaggac acgctctacg gcgcgctctg ccgccgccag
    12661 cgcaaggagt gcgacgaacc gctgccgtat ggcgacgtgg tcggctgcgg ccagatcgac
    12721 gacgtgatcc tggtcgatca gagcccgatc gggcgctcgc cgcggagcaa cccggtgacg
    12781 tatatcaagg ctttcgatcc gatccgcgcg gtgtttgccg aggccgtgga ggcccgcacc
    12841 cacaactaca cggcgggcca cttcagcttc aacgtcgagg ggggccgctg cccggcgtgc
    12901 gagggcgaag gctacacgaa agtcgatatg cagttcctgg ccgacatcta tcgccgctgc
    12961 ggccagtgcg gcggcacccg ctatcgcaag gagatcctcg ccgtcaaata ccgcggccag
    13021 aacatcgcgg acgtgctggg gatgaccgtc cgcgaggctt ttggattctt ccgcggccag
    13081 gcgaaggtgc aggctcgcct caagcagctc atcgacgtgg gcctggacta tttgcagctc
    13141 ggccagcccg ccaacacgct ctcggccggc gaggcccagc ggctgaagct cgcggctcac
    13201 ctttccgagg cgtcgcggaa gcggacgctg ttcctcctcg acgagccgac gacggggctg
    13261 cacttcgccg acgtggtaac gctgctcgat tgcttcgagg cgctgctctc ggtcggccat
    13321 tcgctgatcg tcgtcgagca caatttgcag ctgatgcggg ccgccgatta cctcattgat
    13381 ctcggccccg gcgcgggcga ggatggcgga aggatcgtcg ccaaggggac gccggaagag
    13441 gtcgctgcca acctggcttc gcacacgggg cgatttctcg cccagtcact cctgattggt
    13501 caatcgtcac ttgactgacc tgcgtgagct cgacgtcttt ttcggcgacc tgacggtcaa
    13561 ccgggcgttt gtctatgcgc gcatgccgct gccggcagag gcaggcgatt ggtcgctttc
    13621 cggacaagtg cgcgggccgc gatgcctgac ggctcagacg ctgcccgcca cttcgctgct
    13681 ggaggacttg gggcccggtc cgacgctgat ggcgcgggcc gctgtgcccg atccctgctt
    13741 ctggtcgccc gatctgccgg cgatctacga cctcacggtg aacctgcggc gcggaggcca
    13801 aatcgtcgcc accggcagcc gcgaagttgg gctgcgggct ctgggcgttc gcggccgaca
    13861 tctggccttg gaaagcaagc ggtgggtgct ccgcggcgtc tgcccggaat cgactcacgc
    13921 cgcgttgccg cgtcaatggc atgaagcgct ggcctgctat gtggcggttg atccgcgcgt
    13981 ggaagtgctg gcggaagcgt cgcagtgggg ggcgctggcg gtggttgaag tccgcggcga
    14041 agccgggtcg ctgctggcgc tcttgcgcga gctggcgaag tttcctgcag tcgcacttgc
    14101 ggtcgtgcac ggggagttgc ctgcggagtt caagccctcg caggccgcgc ccaatctgct
    14161 cctggcacag gccatcggcg acggcgacga actggcggtg cgcccttgga ccaacgtctt
    14221 gtgggccgag acggacgact cgctccgcat cggccggatt tccacgctgg ccgagttgcc
    14281 ggtcgtcgcc t
//
LOCUS       DASRJB010000044         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_14890, whole genome shotgun sequence.
ACCESSION   DASRJB010000044 DASRJB010000000
VERSION     DASRJB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14890"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1047
                     /locus_tag="VFB80_01260"
     CDS             58..1047
                     /locus_tag="VFB80_01260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZN32407.1"
                     /translation="MLLLLLALLAPRLAAAQASFAEVAAQTQPKIVKIFGAGGLRSLE
                     AYQSGFLISAEGHILTVWSYVLDSDGVTVYLSDGRKLTATVVGMDPRTEIAVLKIDAD
                     NLPYFNLDEAVPLAAGAKVLAFSNLYGVALGNEPASVLHGIVAAKTDLAARRGAFETN
                     YRGQAYVLDAMTNNPGSAGGALTDRRGRLAGLLGKELRSSTSNIWLNYALPISELATT
                     AHEIVAGKFRPAPRGQEVKRPKEFHTLAALGIHLVPDFLPRTPPFVEMVRAGSPAAKA
                     GVKPDDLILFVNGRVVPSSKLLADELTFIDRLDAVQLTIQRGQDLIELSLTLE"
     gene            1056..>2490
                     /locus_tag="VFB80_01265"
     CDS             1056..>2490
                     /locus_tag="VFB80_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN32408.1"
                     /translation="MARTHLLAAAALLLVPASAPAQVDLQEQEEAAVRAAVEKVAPSV
                     VKIETIGGLERVGKVLVSTGPTTGLAVGEDGYVISSAFNFVQLPSSILVTLPSGKRSA
                     AKIVARDRSRMLVLLKVNTTEKLAVPVAAPRGEMTVGQWAIAVGRTYEQPEPNISVGV
                     ISATNRIWSRAIQTDAKISPANYGGPLIDIQGRVLGVLVPLSPQGAGEIAGAEWYDSG
                     IGFAVPLADINTRLETLKSGQDLHPGQHGLTLKSGDIFSLPAVVAASQAGSPAYKAGL
                     RAGDTIVELDGQKIDRQAQFRHALGPRYAGDKVPVAWLRGKEKQERLQGELELAEKLI
                     PYETPFLGLLPLRAAPGGGVVVRYVYPGSPAAAAGLAAGDRITALGDKAVADAAELRA
                     AVANLEPKTKAILKVERAGQTLTPELTPTKLPTEIPGELPSAVGEPPAPPADKPATGL
                     IEIKLPEETSECVAYIPETYQGNVPHGV"
BASE COUNT          430 a          860 c          832 g          368 t
ORIGIN      
        1 cacgatccag ctccagcagc tcgagctctc cgccgcggag gccaagcctt gaaaagaatg
       61 ctcctgcttt tgctggcgct cctggcgccg cgcctcgccg ccgcgcaggc ctccttcgcc
      121 gaagtcgcgg cccaaacgca gcccaagatc gtcaagatct tcggggccgg cggactccgc
      181 agcctggaag cctatcagag cggttttctg atcagcgccg aggggcacat tctcacggta
      241 tggagttacg tgctcgacag cgacggcgta acggtctatc tgagcgacgg ccgcaagctc
      301 acggccacgg tcgtcggcat ggacccgcgg acggaaattg ccgtgctgaa gatcgacgcc
      361 gacaacctgc cttacttcaa tctcgacgaa gccgtgccgc tggcagccgg agccaaagtg
      421 ctcgccttca gcaacctgta tggcgtggcg ctgggcaacg agccggcgag cgtgctgcac
      481 ggcatcgtcg cggccaagac cgacctggcg gcccgccgcg gcgcgttcga gacgaactat
      541 cgcggccagg cctatgtcct cgacgcgatg accaacaatc cggggtccgc gggcggcgcg
      601 ctgaccgacc gtcgcggccg gctggccggt ctgctcggca aagagctgcg cagctcgacc
      661 agcaacattt ggctgaacta cgccctgccg atttccgagc tggctacgac ggcccacgag
      721 atcgtcgccg gcaagttccg gcccgccccg cgcggccagg aagtgaagcg cccgaaagag
      781 ttccacacgc tggcggccct cggcattcac ctcgtgcccg atttcctgcc gcggacgccg
      841 ccgttcgtcg agatggtccg cgccggctcg cccgccgcca aggccggcgt caaacccgac
      901 gacctgatcc tgttcgtcaa cgggcgcgtc gttccgtcca gcaagctgct ggccgatgag
      961 ctgaccttca tcgaccggct cgacgccgtg caattgacga tccaacgcgg gcaggacctc
     1021 atcgaattat cgctcacact ggaataatcc cgcgcatggc gagaactcat ctgcttgctg
     1081 ctgccgccct gctcctggtg ccggccagcg ctccggccca ggtcgatttg caggagcagg
     1141 aagaggcggc ggtgcgcgcg gcggtggaaa aggtcgcccc ctcggtggtg aagatcgaga
     1201 ccatcggcgg gctggagcgc gtcggcaagg tgctggtcag cacgggaccg acgacgggcc
     1261 tggccgtcgg cgaagacggc tacgtgatct ccagcgcttt caacttcgtc cagctacctt
     1321 cgtcgatcct ggtgacgctg cccagcggga aacgctccgc ggcgaaaatc gtcgcccgcg
     1381 accgcagccg gatgctggtt ctgctcaagg tcaacacgac cgagaagctg gccgtgcctg
     1441 tggccgcgcc gcgcggcgag atgaccgtcg gccagtgggc gatcgccgtg gggcggacct
     1501 acgagcagcc ggagccgaac atctccgtgg gcgtcatcag cgcgacgaac cggatctgga
     1561 gccgggcgat ccagaccgac gccaagattt cgccggcgaa ttacggcggc cccctgatcg
     1621 acatccaagg acgcgtgctg ggcgtgctcg tgccgctgtc gccgcaaggc gccggcgaga
     1681 tcgccggcgc ggaatggtac gactcgggga tcggctttgc cgttccgctc gccgatatca
     1741 acaccaggct ggagacgctg aagagcggcc aggacctgca tccgggccag cacggtctga
     1801 cgctcaagtc gggcgacata ttctccctgc cggcagtggt cgccgcgtcg caggccggct
     1861 caccggccta caaggcgggc ctccgcgcgg gtgacacgat tgtcgagttg gacggccaga
     1921 agatcgatcg gcaggcgcaa ttcaggcacg ccctcggccc gcgctatgcc ggcgacaagg
     1981 tgccagtcgc ctggctgcgc ggcaaggaaa agcaagagcg gctccagggc gagctggagc
     2041 tggccgagaa gctgattccc tacgagacgc cgttcctggg cctgctgccg ctgcgggccg
     2101 cgcccggcgg cggagtcgtc gttcgctacg tctatcccgg cagccccgcg gctgccgcgg
     2161 gcctggcggc cggcgatcgc atcacggccc tcggcgacaa ggcggtggcc gacgccgcgg
     2221 agcttcgcgc ggccgtggcc aacctggagc cgaaaaccaa ggccattctg aaggtcgagc
     2281 gggccggcca gacgctgacg cccgagctta caccgacaaa gctgcctacc gagattccgg
     2341 gcgaattgcc gtcggccgtc ggcgagccac ccgcgccgcc cgccgacaag ccggcgacgg
     2401 gactgatcga gatcaagctg cccgaagaga cgagcgagtg cgtcgcctac attcccgaaa
     2461 cgtaccaggg caacgtgccg cacggcgtgg
//
LOCUS       DASRJB010000045        13037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3682821, whole genome shotgun sequence.
ACCESSION   DASRJB010000045 DASRJB010000000
VERSION     DASRJB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3682821"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..946)
                     /locus_tag="VFB80_01270"
     CDS             complement(122..946)
                     /locus_tag="VFB80_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32409.1"
                     /translation="MESTALGPPDLSESLVACSQPFVGRWNKLVSTTNWEKGRIIVQW
                     REALIAQGLAVSEYSDEAWSRLVGGVTGQHAGRLRRVTQRFGAAAEQYPGLYWSHFQA
                     ALDWSDAEMWLEGARQSGWSVAQMREQRWQTLGALEADRPRPEDEIAGETDEDFEPAR
                     RQEPIPQTITGEYDDVSGPRHEGPDFGDDERAAAPGGTALEWEVPVTGEAAPLVRPFE
                     NLPELPDDLAEAFDAMKLAILHHKQDGWQRTSAGDVLRTLDALKALVTAPSDAAPF"
     gene            complement(1177..1575)
                     /locus_tag="VFB80_01275"
     CDS             complement(1177..1575)
                     /locus_tag="VFB80_01275"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HZN32410.1"
                     /translation="MPTPIAIAVVEWQGQFLVGQRPPGVPLAGLWEFPGGKIEPGESP
                     EDAAIRECREEAGLRVEPVSRYPECVQQYDHDRLTLHFIACRPAASAALEPRPPFRWI
                     PRGELSQYEFPRGNRGVLEIIAAGEASTGS"
     gene            complement(1679..2503)
                     /locus_tag="VFB80_01280"
     CDS             complement(1679..2503)
                     /locus_tag="VFB80_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZN32411.1"
                     /translation="MGMFDGKKGLILGVANDRSIAWAIAQVILNEGGVCGFNHLPDKP
                     DDERQRNRRRVTQLTDPFGDKVKFVLPLDVQKDDDIAAFMAKAKAELGQIDFLLHSIA
                     FATLEDLKKETTATSREGFKLAMEISAYSLLAVVNAAREIFSPQSSVLTLTYYGGERI
                     VPGYNVMGICKAALDACMKYAAFDLGPKGVRVNALSAGPLKTLASSAVGAKGMEELYR
                     EVSPMARNVTHDEVGRVGAFLLSPMSDGITAEILHVDAGYNAMGSPGRLLDKLKTL"
     gene            2646..4565
                     /locus_tag="VFB80_01285"
     CDS             2646..4565
                     /locus_tag="VFB80_01285"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZN32412.1"
                     /translation="MNRPATSPDALPADAAARLYYLTALDLEPAALLRQAIPIACEAL
                     ALDYLAVVQGEKGQWRILESSQPQQRLPEALLADALDQGQPIAKGEWYAAPLEPGGGE
                     LLAAQRKKGSWQDRGLLDALRQWLARALALSRGRQRDKQQIARQAAILEIAAQWQQTL
                     EMDALLTRMAEASTKLLAAERASIFLWDKAAKTLVGRPALGAPGNELRIPDDAGVVGQ
                     VVQTGQPRRVDSDVAAEQRDIDRRVDQKLKFQTRSIVCVPLRGKKGDLFGAFEVLNKR
                     GGNFTPDDEQALLELAAHAAVALENTQQWEQLLAARKQMADQAEQGVQLIGESPAVAA
                     LRSTIHRVADTELAVLVLGENGTGKEVVAQMIHYKSRRRQEPFIAVNCAALTETLLES
                     ELFGHEKGAFTDAREMRRGKFELAASGTLFLDEIGDMSLGGQAKLLRVLEEKVVVRVG
                     GSTPIPTNTRVIAATNQNLAELVRSRRFREDLYFRLNVVTLDLPPLRERGEDVLLLAE
                     HFLKHFAAQARRKSLKFTAAAKKRMLAHSWPGNVRELRNMMERLAYLAPEEQDKIDAP
                     DLAFILSPDASSAGPLSLDAPLAEATRQFQSEYIQRHIERLRGNMTAAAEKLGLHRSN
                     LYRKMRQLGMEVAEE"
     gene            complement(4651..5643)
                     /gene="fliG"
                     /locus_tag="VFB80_01290"
     CDS             complement(4651..5643)
                     /gene="fliG"
                     /locus_tag="VFB80_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZN32413.1"
                     /translation="MPDIRKAAILLMSLPEEQAAQLMSKLTPKQVEQVSIEIAKLGRL
                     GADEQEQVIHEFADSNPAALGIDGGGIDLAKSLVERALGNKAGQTLDVVRQSIEALPF
                     GFLKKVDPQNLMTFILDEHPQTIALVLSHLPPAYGAEIIKGLPAERQLAVIRRIAHMG
                     QTNPEVIQEVERGLETRMSSLMNQSFEKAGGVNSVAEILNVSDRAVERVLLESLSQED
                     PELVEEIRRLMFVFDDLCKLSDRDIQTVLKNVETAQWAMGLKGASAELKEKILGNMSQ
                     RASALLVEEMEYLGAVKLSEVEAVQQQIVDIVRRLEDAGEITVHAADQSEQMIT"
     gene            complement(5728..6483)
                     /locus_tag="VFB80_01295"
     CDS             complement(5728..6483)
                     /locus_tag="VFB80_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32414.1"
                     /translation="MNRLTASTLLASPAPAVARAQRPQLHKDHSAPPHCLLAPQHYEK
                     NYAYPLVVWLHPTGGDELELRQVMPLISLRNYASAAVRGPAVLRAGYDWPQTLGGIGM
                     AETRVAEAASQAKLRFNVHDGRVYLLGYEAGGTMAIRLALRNPRLYAGAVSLNGPFPA
                     GHAPLARLPQLRQTRILISHCRDSETYTTDRICRELALFHAAGMSATLRQYPCSDELT
                     TQMLSDVDGWLMEQITGERSDAEPQDALPGEWN"
     gene            complement(6877..7827)
                     /gene="mdh"
                     /locus_tag="VFB80_01300"
     CDS             complement(6877..7827)
                     /gene="mdh"
                     /locus_tag="VFB80_01300"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZN32415.1"
                     /translation="MGRAKITIVGAGNVGATCAHWCAAAELGDIVLLDIPQTGDMPKG
                     KGLDLMQAGPIMGFDSNIVGCTDYGPSAGSDVVVITAGIARKPGMSRDDLLSTNAKIV
                     SSVAESVKATSPKAVVIVVSNPLDAMVQRAFQVTGFPPQRVCGQAGVLDTARYRTFLA
                     MELGVSVEDVSALLMGGHGDTMVPMPSCTSVGGIPVRRLLSEDKLNAIVDRARSGGAE
                     IVALLKTGSAYYAPAAATAQMVEAIVRDKKRLIPCAAYCDKEYGVGGYYVGVPVILGA
                     GGVEKIVELQLDPQERSAFDNSVSAVKSLVKTMGELLAKV"
     gene            8061..9515
                     /locus_tag="VFB80_01305"
     CDS             8061..9515
                     /locus_tag="VFB80_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HZN32416.1"
                     /translation="MFSFFARRRRQKLLRQPLPAAWREILERNVAVYSRLSSVERSRL
                     EAALQVIASERRFVGCGGLVITDEVKVTIAAQAALLLLGEEGYYFDRVPTIFVFPRPR
                     ADKVGRNVQHGSAFSAGAHVVEEGVIVEGQAYAQGEIRLAWSEVLAGGRDAGDGENVV
                     LHELAHHLDGLDGEMGGTPPLPDEAARIHWQQVFDAELVQLRRDLATDRPALFHDAAA
                     DSRTELFAYATEVFFEQPIELGEEHAELFECLRDFYKVDPTRWFAAQPADAPFTRPFE
                     AAVPRVPLPSEDDDWEEQEEPAAELPPLETADQYFTRGLESFEREDFEAAEADFDRAV
                     KLAPQDQEAVLYRGRARMYLSDTQAALADAERACRLAPDDLEAIALRGICQVALGEFA
                     EGLADLENAADALDGDLDLLYSLGVARAETGQTAAAIAAFTRLIQLDPKDAEAWHERS
                     QCHAELGDKSAAERDLAQARALGWEEGQKDGETE"
     gene            9707..10453
                     /locus_tag="VFB80_01310"
     CDS             9707..10453
                     /locus_tag="VFB80_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32417.1"
                     /translation="MLTRRQFAQSTLGSLLTYSLLETLIAQDAFGADLKVQAAKWLKD
                     VNDWSQDIKGAKLPQVEWQKKVDELLGQVDLSDTLKLLDFEKLTANLKLKDQGELSLH
                     ATLPKVEGLPTSLVFGHQVFALGKDRSVVPHGHNNMATAFLILKGSFRGRHYDRLEDD
                     GQFMIIRPTIDRSFTTGEHSTVSDFKDNIHWFTATSEQAFIFNIHVNGVDPKRKDTGR
                     VYLDPNGEKLSDGRIRAKRVTSGEVYKLYG"
     gene            10614..11399
                     /locus_tag="VFB80_01315"
     CDS             10614..11399
                     /locus_tag="VFB80_01315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN32418.1"
                     /translation="MQLHLASQVAVVVGGGRGIGRAIADFFAAEECRVAIVDRELSAA
                     PAGPRTTCLQADATDYAAVQAAAKQIEQALGKVQHVVYAAGCGSGKFGFPFWNLQPAD
                     WDKVLRVNLVGAVNAAHAFAPTLVEQQRGTLLFISSVAGQIGSQTDPPYSAAKAGLIN
                     FAQCAAKDLAPYSVRVNTICPGMVKTDLNRGIWQAWHDQQPAELRRSYDEWALEKIRK
                     VCPLGRWQTPEEIAALAVFLASDRACNITGQTMNVDGGFVMHW"
     gene            11534..12589
                     /locus_tag="VFB80_01320"
     CDS             11534..12589
                     /locus_tag="VFB80_01320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN32419.1"
                     /translation="MGIEDRDYTRDSSDYTGTLTGFGLDYIPPVVKWIIIANVVVFVA
                     QIFVVRERTPADAKAALDRLPKKQRELVEKQRDAQRKMYEKEGLDPADYEDVAWNGEN
                     NVSMIQEWFQLEARQVARGQVWRLLTYAFCHDRFGLWHILFNMLLLYWCAVTLESMLG
                     PREFLFFYLVAAVFAGLVEFAFNLYLGSSIPVVGASGAVMAVGMLYAIHYPRTTIRLF
                     WFWPIEARWVVLFYVLYNLHPLLLALGGTQYFTGSAFACHLGGLAFGFLYYKLNLNLE
                     RWWDLLPKPRGSFAWSGSGARRPPRRASARREPSIDDQVDEILRKIHESGRESLTDAE
                     RRLLDRASQRYKDRRDV"
     gene            12722..>13037
                     /gene="tnpA"
                     /locus_tag="VFB80_01325"
     CDS             12722..>13037
                     /gene="tnpA"
                     /locus_tag="VFB80_01325"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HZN32420.1"
                     /translation="MSTYICNLVHFVWGTANREPLLQKSWRDRLHGYIGGILENKRAK
                     LLAAGGIADHIHVLASLPATLSLADAANAMKANSSRWIHENAPQSKGFDWQEGYGAFS
                     VSK"
BASE COUNT         2258 a         4286 c         4314 g         2179 t
ORIGIN      
        1 aggaatggga tgaagattag gatcagaaag tctaatctta atcgtaatcc taatcctctc
       61 gctggccgac cctggccctc tcccactgcg gattaagata ggattgagat tacgattagg
      121 atcagaacgg cgcggcgtcc gacggcgcgg tgacgagggc cttgagggca tcgagcgtcc
      181 gcagcacgtc gccggcgctg gtccgctgcc agccgtcctg cttgtggtgc aggatcgcca
      241 gcttcatcgc gtcaaaggcc tcggccaggt cgtcgggcag ctccggcaag ttctcgaacg
      301 ggcggaccag cggggccgct tcgccggtga cgggaacctc ccactcgaga gccgtcccgc
      361 cgggcgctgc tgctcgctcg tcgtcgccga agtccgggcc ttcatggcgc gggccgctga
      421 cgtcgtcgta ttcgccggtg atcgtctgcg ggatcggctc ctgccgccgc gccggttcaa
      481 agtcctcgtc ggtctcgccg gcgatctcgt cctcgggccg cgggcggtcg gcttcgagcg
      541 cgcccagcgt ctgccaccgc tgctcgcgca tctgggcgac cgaccagccg ctctgccgcg
      601 cgccctccag ccacatctcg gcgtcgctcc agtcgagcgc cgcctgaaag tggctccagt
      661 acagccccgg atactgctcg gccgccgcgc cgaaccgctg ggtcacgcgc cgcagccggc
      721 ccgcgtgctg tccggtgacg ccgccgacca gccggctcca ggcctcatcg gaatactcgc
      781 tgacggccag gccctgtgcg atcagggcct cgcgccactg cacgatgatc cgccccttct
      841 cccagttcgt cgtgctgacc agcttgttcc agcggccgac gaacggctgc gaacaggcga
      901 ccagcgattc actcagatcc ggcggcccaa gagcggtcga ttccacggag gcgcttccct
      961 gctaggacgt ttcggcggag gagctaacgt agcaatttcc gccgcgaggg aaacccctca
     1021 cgatctcgcc gtgaaaagcg gccctggccg actcgccggt ggggacaacc tgtcgatgca
     1081 gtcatttgtc acttgtcatt tgtcatttgt catttgacgg tggaaacaca tgacaaatga
     1141 ccaatgacca gtgaccgatg acgaaacgac tcattcctaa gatcccgtgc tcgcttcgcc
     1201 tgcggcgatg atctccagga cgccgcggtt gccgcggggg aattcgtact gggaaagctc
     1261 gccccgcgga atccagcgaa aaggcggccg cggttcgagc gcggcgcttg cggcgggccg
     1321 gcaggcaatg aagtgcagcg tcaggcggtc gtggtcgtat tgctggacgc actcgggata
     1381 acgcgagacc ggctcaactc gcaaacccgc ctcttcgcgg cactcgcgga tcgcggcgtc
     1441 ctccggcgat tcgcccggct cgatcttgcc gccgggaaac tcccacagac ccgcgagcgg
     1501 cactccgggc ggccgctggc ccacgagaaa ctgcccctgc cactcaacga cggcgatcgc
     1561 aatcggcgtg ggcatctcaa agccgcgacc attggtcgcg gtttacaata tgggccggcg
     1621 gcgccaatag accgcgacca ctggtcgcgg ctttgaggac cgccacggag ggcattccct
     1681 acagcgtctt cagcttatcc agcagccggc cgggcgaacc cattgcgtta tagccggcat
     1741 cgacgtgaag gatttcggcg gtgatgccgt cggacatcgg agagagcaag aacgccccga
     1801 cgcggcccac ctcgtcatgc gtcacgttgc gggccatggg cgagacttcc cgatacagct
     1861 cttccatccc cttcgcaccg accgccgaac tggccagcgt cttcagcgga ccggccgaga
     1921 gtgcgttgac ccgcacgccc ttggggccca ggtcgaacgc ggcgtacttc atgcaagcgt
     1981 cgagcgccgc cttgcagatg cccatcacgt tgtagcccgg cacgatccgc tccccgccgt
     2041 aatacgtgag cgtcagcacc gacgactgcg ggctgaaaat ctcccgggcc gcgttcacga
     2101 ccgccagcag gctgtacgcg ctgatctcca tcgccagctt gaagccctcg cggctggtcg
     2161 cggttgtctc tttcttgaga tcctcgagcg tggcgaacgc gatcgaatgc agcaaaaagt
     2221 cgatctgccc gagctcggcc ttggccttgg ccatgaaggc cgcgatgtcg tcgtccttct
     2281 ggacgtcgag cggcaggacg aacttcactt tgtcgccaaa cggatcggtg agttgcgtca
     2341 cccggcggcg gttgcgctgc cgttcgtcgt cgggcttgtc gggcaggtgg ttgaagccgc
     2401 agacgccccc ctcgttcagg atcacttgcg caatggccca ggcgatcgag cggtcgttgg
     2461 ccactcccag aatcagcccc tttttcccgt cgaacatccc catcggtggt ctcctcagcg
     2521 tgccatgaag atatcacgcc gcccggcctc ggaccacgcc agccaggtgt cgcaaaatcg
     2581 atacacgccg gggaagcggg tcgtataatc tgatagcgct ccgcgatcaa ttagacgaca
     2641 gtgccatgaa ccgccctgca acctcgcccg atgccctccc cgccgacgct gcggcccgac
     2701 tctactacct gacggcgctc gacctggagc cggcggcact gttgcggcaa gcgatcccaa
     2761 tcgcctgcga agcactggcg ctcgattatt tggccgtcgt ccagggcgaa aaggggcaat
     2821 ggcggatcct cgagagttcc cagccgcagc agcggctgcc ggaggcgctc ttggccgacg
     2881 cactcgatca gggccagccg atcgccaagg gagagtggta cgctgccccg ctggagccgg
     2941 gcggcggcga attgctcgcc gcgcagcgaa agaaaggaag ctggcaggac cgcggcttgc
     3001 ttgatgcgct gcggcagtgg ctggcccgcg cgctggccct cagtcgcggg cggcagcgcg
     3061 acaagcagca gatcgcgcgg caggcggcga ttctggagat cgcggcgcag tggcagcaga
     3121 cgctggagat ggacgccctg ctcacgcgga tggccgaggc ttcgacgaag ctgctggcgg
     3181 ccgagcgggc cagcattttt ctgtgggata aggccgcgaa aacgctggtc ggccggccgg
     3241 cgctgggcgc cccagggaac gagctgcgaa tcccggacga cgcgggcgtc gtggggcagg
     3301 tcgttcagac ggggcagccg cggcgggtcg atagcgacgt ggccgcggag cagcgcgata
     3361 tcgaccgaag agtcgatcag aagctcaagt tccagacgcg gtcgatcgtg tgcgtgcccc
     3421 tgcgcgggaa gaagggggac ctgttcggcg cgttcgaggt gctcaacaag cggggcggca
     3481 atttcactcc cgacgacgag caggcgctgc tcgagctggc cgcgcacgcc gcggtcgcgc
     3541 tggaaaacac gcagcagtgg gagcagctgc tggcggcgcg gaagcaaatg gccgaccagg
     3601 ccgagcaggg cgtgcagctc atcggcgaga gcccggccgt cgcggccctc cgctcgacga
     3661 tccaccgcgt ggccgacacg gaactggcgg tgctggtgct gggcgaaaac ggcaccggca
     3721 aggaagtcgt ggcccagatg atccactaca agagccggcg gcggcaggag cccttcattg
     3781 ccgtcaattg tgcggcgctg accgagactt tgctggagag cgagctgttc gggcacgaaa
     3841 aaggggcctt caccgacgcc cgcgagatga ggcgcggcaa gttcgagctg gcggccagcg
     3901 gcacgctgtt tctggacgag atcggcgaca tgagcctggg cggccaggcg aagctcctcc
     3961 gcgtgctgga agagaaagtc gtcgtccgcg tcggcggcag cacgccgatc ccgaccaaca
     4021 cccgcgtcat cgcggcgacg aaccagaacc tggccgagct ggtccgctcg cggcggttcc
     4081 gcgaggacct ctacttccgg ctgaacgtgg tcacgctcga cctgccgccc ctgcgcgagc
     4141 ggggcgagga cgtgctgctc ttggccgagc atttcctcaa gcacttcgca gcgcaggccc
     4201 gccgcaagtc gctcaagttc acggccgccg ccaagaagcg gatgctggcg cactcctggc
     4261 ccggcaacgt ccgcgagctg cggaacatga tggagcggct ggcgtacctc gcgccggaag
     4321 agcaggacaa gatcgacgcg ccggacctgg cgttcatcct ttcgcccgac gcctccagcg
     4381 ccgggccgct atcgctcgac gcgccgctgg ccgaagcgac gcggcaattt cagagcgaat
     4441 acatccagcg gcacatcgag cgcctccgcg gcaacatgac ggccgccgcc gagaagctcg
     4501 gcctgcatcg cagcaatctg taccgcaaga tgcggcagct ggggatggaa gtggcggagg
     4561 agtagatcgg tggtcggtca gtcggtcggt cggttagtcg gtgcatttga cctactgacc
     4621 aaccgacgga ctgaccgacc gactgaccgg ctacgttatc atctgctcgc tctggtccgc
     4681 cgcgtggacc gtgatctcgc cggcgtcttc gaggcggcgg acgatatcga cgatctgctg
     4741 ctggacggct tcgacttcgg agagcttgac cgcgcccagg tattccattt cctcgaccag
     4801 gagggcactg gcccgctgcg acatgttgcc caggatcttt tccttcagct cggcgctggc
     4861 gcccttcagg cccatcgccc actgggcggt ctcgacgttc ttcagcaccg tctgaatgtc
     4921 cctgtcgctg agcttgcaga ggtcgtcgaa gacgaacatc aggcggcgga tttcctcgac
     4981 cagctcaggg tcctcttgcg acagcgattc cagcagcacc cgctccacgg cccggtccga
     5041 gacgttgagg atctccgcca cgctgttgac gccgccggcc ttttcgaacg actggttcat
     5101 caggctcgac atgcgggtct ccaggccgcg ctcgacttcc tggattacct cgggattcgt
     5161 ctggcccatg tgggcgatgc ggcggatcac cgccagctgc cgctcggcgg gcaatccctt
     5221 gatgatctcc gctccgtaag ccggcggcag gtgcgagagc accagggcga tcgtctgcgg
     5281 atgctcgtcg aggatgaacg tcatcaggtt ctgcggatcg acttttttca ggaagccgaa
     5341 cggcaaggct tcgatcgact ggcggacgac gtcgagcgtc tggccggcct tgttgccgag
     5401 ggccctttcg acgagcgact tggccaggtc gatcccgccg ccgtcgatgc cgagcgccgc
     5461 cgggttgctg tcggcgaact cgtggatgac ctgctcctgc tcgtcggcgc ccaagcggcc
     5521 cagcttggcg atttcgatcg agacctgctc gacctgcttg ggcgtgagtt tcgacatcaa
     5581 ctgcgccgcc tgctcctccg gcaggctcat cagcaggatc gcggctttgc ggatatcggg
     5641 catagggggc cagggcggga ccaggagcca ggggccgcgc gcggagtatc cgcgaaagag
     5701 agcgaccctg tcgaggtcag atgcgcgcta gttccactcg ccaggcagcg cgtcctgcgg
     5761 ctcggcgtcg ctccgctcgc cggtgatctg ctccatcagc cagccatcga cgtcgctgag
     5821 catctgcgtc gtgagctcgt ccgaacaggg atactggcgg agcgtggccg acatgccggc
     5881 cgcgtgaaac agggccagct cccggcagat gcggtcggtc gtataagtct ccgaatcgcg
     5941 gcaatgcgag atcaagatcc gcgtctggcg aagctgcggc agccgggcaa gcggcgcatg
     6001 cccggcaggg aacgggccgt tgagcgaaac cgcgccggcg tacagccgcg ggttgcggag
     6061 ggccaggcgg atggccatcg tgccccccgc ttcgtagccg agcagataga cgcggccgtc
     6121 gtgaacgttg aaccgcaatt tggcctggct ggcggcctcg gccacgcgcg tttcggccat
     6181 accgatgccg ccgagcgttt gcggccagtc gtagccggcg cgcagcacgg cgggtccgcg
     6241 gacggcggcc gacgcataat ttcgcaggct gatgagcggc atcacctgcc gcaattcgag
     6301 ctcgtcgccg cctgtggggt gcaaccagac aacgagcgga taggcatagt tcttttcgta
     6361 atgttgcgga gcgagcaggc agtggggcgg ggcggagtgg tccttatgaa gctgcggccg
     6421 ctgtgctcgg gcgaccgcgg gggcggggct ggccagaagc gtggaagcag tgagtcggtt
     6481 cataaactct gggtcacgag ggaggaaaga gcgccgcgca cgggttggga cccgcgcgag
     6541 caccagtccg cgtgcagcag tttgcaagcg gcgcgccgcg gccaatgcca gccgaaagcg
     6601 ggacggccgg caatcgccgg gaagtgggac cgcgagtgga ttcgctggcg gtcaaaggac
     6661 ggattccgtc gaggtgagga gcgacgcggg ggcggccggc actggtgggt atgatggcgg
     6721 gctgccaggc gtcgtgtcgc cgggccggga ctccaatccg ggagccgggc cagacgagcg
     6781 ggttgtatcg gggcctatgc atcctgtcaa cgccaagggc ggattgccgc acgctagcgg
     6841 tgctagcacg ctgattggcg cgcggcatgc agcgcactac actttcgcca acagctcgcc
     6901 catcgtcttg acgagcgact tcacggcgct gacgctgttg tcgaacgctg accgctcttg
     6961 cgggtcgagt tggagttcga caatcttttc aaccccgccg gcgcccagaa tgaccggcac
     7021 gccgacgtaa tagccgccga cgccgtactc tttgtcgcaa tacgccgcgc agggaatcag
     7081 ccgcttcttg tcgcggacga tcgcctcgac catctgggcc gtcgcggcag cgggagcata
     7141 ataggcgctg ccggtcttga gcagcgcgac gatctcggcg ccgccgctgc gggcccgatc
     7201 gacgatcgca ttcagcttgt cctccgagag cagccgccgg acgggaatgc cgccgacgct
     7261 ggtgcagctc ggcatcggca ccattgtatc gccgtggccg cccatcaaga gcgcggagac
     7321 gtcctccacg ctcacgccca gctccatcgc caggaacgtg cgatagcggg ccgtgtcgag
     7381 cacgcccgcc tggccgcaga cccgctgcgg tggaaaaccc gtcacttgaa acgcccgctg
     7441 caccatcgcg tccagcggat tgctgaccac gatgaccacc gccttgggac tggtggcctt
     7501 cacgctctcg gccaccgacg agacgatctt cgcattggtg ctgagcagat cgtcgcggct
     7561 catgcccggc ttgcgggcga tgccggccgt gatcacgacc acgtcgctgc cggccgacgg
     7621 gccgtaatcg gtgcagccga cgatgttcga atcgaagccc atgatcgggc cggcctgcat
     7681 caggtcgagg cccttgccct tgggcatgtc gcccgtttgc ggaatgtcga gcaggacgat
     7741 gtcgcccaac tcggccgcgg cgcaccagtg ggcgcaggtc gcccccacgt tgcccgcgcc
     7801 cacgatcgtg atcttggctc ggcccatagg cggctccttc gaggacaacc gaatatgagc
     7861 taccggcgcg gagcgtcaag ggggcgggag tcggtcagtc ggtgatcggt cagtcggtgg
     7921 tcggcgcgtg caggcgctgg gtcgctcgct gcgtactctg tactcagtac tcggtactga
     7981 gtaccaagta cgaagcgacg gcctccgcga aacaactcac cgactcaccg accgaccgac
     8041 caccgaccac tcactccccc gtgttctcct tcttcgcccg ccgccgccgc cagaaactgc
     8101 ttcggcagcc gctgcccgcg gcgtggcgcg agattctcga aagaaacgtc gcggtctatt
     8161 cgcggctgtc ctcggtggaa cgctcgcggc tggaagcggc gctgcaggtg atcgcgagcg
     8221 agcggcggtt cgtgggctgc ggcggcctgg tgattaccga cgaagtgaag gtcacgatcg
     8281 cggcccaggc agcgctgctg ttgctcggcg aggaaggcta ctacttcgac agggtgccga
     8341 cgattttcgt ctttccgcgg ccgcgggccg acaaggtggg gcggaacgtg cagcacggct
     8401 cggcgttttc ggccggggca cacgtcgtcg aagagggtgt catcgtcgag ggccaggcct
     8461 acgcgcaggg tgaaatccgc ctggcgtgga gcgaagtgct cgcgggcggg cgcgacgccg
     8521 gcgacggcga gaacgtcgtc ctgcacgaac tggcccacca tctcgacggg ctggacggcg
     8581 aaatgggggg cacgccgccg ctgccggacg aagcggctcg cattcattgg cagcaggtgt
     8641 tcgacgcgga gctggtccag ctccgccgcg acctggcgac ggatcggccg gcgctgtttc
     8701 acgacgcggc ggccgacagc cgcacggagc tcttcgccta tgcgaccgag gtattcttcg
     8761 agcagccgat cgaactgggc gaggagcacg ccgagctgtt cgagtgcctg cgggactttt
     8821 acaaggtcga tccgacgcgc tggtttgcag cgcagccggc ggacgcgccc ttcactcggc
     8881 cgtttgaggc cgcggtcccc cgcgtgccgc tgccgagcga ggacgacgat tgggaggaac
     8941 aggaagaacc ggcggcggaa ctgccgccgc tggagacggc cgaccagtat ttcacgcggg
     9001 gactggagtc gtttgaacgg gaggactttg aggcggccga agctgacttc gaccgggcgg
     9061 tgaagcttgc gccgcaggac caggaagcag tgctctaccg cggccgcgcg cgaatgtatc
     9121 tcagcgacac gcaggcggcg ctggccgacg ccgagcgggc ctgccgcctg gcgccggacg
     9181 acctggaagc gatcgccctc cgcggcatct gccaggtcgc cctgggcgag tttgccgagg
     9241 gcctcgccga cttggagaac gccgccgatg cgctggatgg cgacctcgac ttgctctatt
     9301 ccctgggcgt ggcccgcgcc gaaacgggcc agaccgccgc cgccatcgcc gccttcacgc
     9361 ggctgatcca gctcgacccc aaggacgccg aagcctggca cgagcggtcg cagtgccatg
     9421 ccgagctggg ggataagtcc gctgccgagc gggatttggc gcaggcgcga gcgctgggat
     9481 gggaagaggg acagaaggac ggagagacgg agtgaaagag agagggaaag agggagtcct
     9541 aatcctaatc ttaatcgtaa tcctaatccc tccgccgctg cccacgttcc acggaccgag
     9601 caggattagg attaagatta cgattaggat taggatccgt cactgcagct tgactgttcc
     9661 cggcgccact tggatcatac aggcacctca accaggagcc cgcgcaatgc tcacccgccg
     9721 ccagttcgct cagagcacgc ttggatcgct gctcacctat tcgctgctcg aaacgctgat
     9781 cgcccaggat gcgttcggag cggacctcaa ggtccaggcg gcgaagtggc tcaaggatgt
     9841 gaacgactgg agccaggaca tcaaaggcgc gaagctgccg caggtcgagt ggcagaagaa
     9901 ggtcgatgag ctgctggggc aggtggacct gtccgacacg ctcaagctct tggacttcga
     9961 gaagctcacc gccaacttga aactgaagga ccagggggag ctgagcctgc acgccacgct
    10021 gccgaaggtc gaagggctgc cgacgagcct ggtcttcggc caccaggtct tcgccctcgg
    10081 caaggaccgt tcggtcgtgc cgcacggcca caacaacatg gccacggcgt tcctgatcct
    10141 caagggctca ttccgcggcc ggcactacga ccgcctggag gacgacgggc agttcatgat
    10201 catccgcccg acgatcgacc ggagcttcac gaccggcgag cactcgacgg tgtccgactt
    10261 caaggacaac atccactggt tcaccgccac cagcgagcag gccttcatct tcaacatcca
    10321 cgtcaacggc gtcgatccca aacgcaagga caccggccgc gtctatctcg accccaacgg
    10381 cgaaaagctg tccgacggcc gcatccgcgc caaacgcgtc accagcggcg aggtctacaa
    10441 actctacggc tgaagcggag ccgggaccaa tttcggcctg cagccgtgcg acaatcccga
    10501 aaatgctcac acagaggcac aggggcacag agcagcaggc agcgctactg gggttcagcc
    10561 tctcctctgt gcctccgtgc ctctgtgtga gatgaatatg cgttgttgat gacatgcaac
    10621 tccacctcgc cagccaggtc gccgtggtcg tcggaggcgg gcgggggatt gggcgggcga
    10681 ttgcggattt ctttgcggcg gaagagtgcc gcgtggcgat cgtcgatcgc gagctgtccg
    10741 ctgctccggc cgggccgcgg accacctgcc tgcaggcgga tgcgaccgac tacgccgccg
    10801 tgcaagcggc agctaagcag atcgagcagg ccctcggtaa ggtgcagcac gtggtctatg
    10861 ccgcgggctg cggcagcggc aaattcggct ttccgttctg gaacctgcag ccggcggatt
    10921 gggacaaggt cttgcgggtg aacctggtcg gtgccgtaaa cgcggcgcat gctttcgcgc
    10981 cgacactggt cgagcagcag cgcgggacgc tgctcttcat ctcgtcggtc gccgggcaga
    11041 ttggctcgca gaccgatccg ccctacagcg ccgcgaaggc cgggctgatc aacttcgcgc
    11101 agtgtgcggc gaaagacctc gcgccgtaca gcgtccgcgt caacacgatc tgccccggca
    11161 tggtgaagac cgacctcaac cgcggcatct ggcaggcctg gcacgaccag cagcccgccg
    11221 agctgcgccg cagctacgac gagtgggccc tggaaaaaat ccgcaaggtc tgcccgctcg
    11281 gccgctggca gacgcccgag gagatcgccg ccctggccgt gttcctcgct tccgaccgcg
    11341 cctgcaacat caccggccag acgatgaacg tggatggtgg gtttgtgatg cactggtagg
    11401 agtgagtggt cggtggtcgg tggcggtggt cggtagtaag aaatcggttg gccggcaccg
    11461 actgaccgac cgactgaccg actaaccggc ctaccactca ccactcacca cttacgcgac
    11521 ctacaatttc cccatgggca tcgaagaccg cgattacacc cgcgactcgt cggactacac
    11581 cggcacgctg accggcttcg gcctcgacta catcccgccg gtcgtcaagt ggatcatcat
    11641 cgccaacgtg gtcgtcttcg tggcccagat cttcgtcgtc cgcgaacgta cgccggccga
    11701 cgctaaggcc gcgctcgacc gccttcccaa gaagcagcgc gagctcgtgg agaagcagcg
    11761 cgacgcgcag cggaagatgt atgagaagga ggggcttgat cccgcggact acgaagacgt
    11821 cgcctggaac ggcgaaaaca acgtctcgat gatccaggag tggtttcagc tcgaggcgcg
    11881 gcaggtcgcc cgcgggcagg tctggcggct gctcacgtac gcgttctgcc acgaccgctt
    11941 cgggctgtgg cacatcctct tcaacatgct gctgctttac tggtgcgcgg tcacgctcga
    12001 gtcgatgctt ggcccgcgcg agttcctgtt cttctatctg gtggcggcgg tctttgcggg
    12061 cctcgtcgaa ttcgccttca acctctatct cggcagctcg attcccgtcg tcggcgcgtc
    12121 cggggccgtg atggccgtgg gcatgctcta cgccatccac tatccgcgga cgaccatccg
    12181 gctgttctgg ttctggccga tcgaggcccg ctgggtcgtc ttgttctacg tgctctacaa
    12241 cctgcacccg ttgctcctgg ccctgggcgg gacgcagtac ttcaccggct cggccttcgc
    12301 ctgccacctc ggcggcctgg cgtttggctt cctgtattac aagctcaatc tcaacctcga
    12361 acgctggtgg gacctgctcc ccaagccccg cggctccttc gcctggtccg gaagcggcgc
    12421 ccgccgaccg ccgcgccgcg ccagtgctcg ccgcgagccc agcatcgacg accaggtcga
    12481 tgaaatcctc cgcaagattc acgaatccgg ccgcgaatcc ctcaccgacg ccgagcgccg
    12541 cctcctcgac cgcgccagcc agcgctacaa ggaccggcgg gacgtctgac gtagggcagg
    12601 ctgccagcct gccttaggcc cggagggccg gcaggtaata gccccgaccg tcaggtcggg
    12661 gtatcgtgtc gccaaatacc ttgtgaggtc cggaggaccg acacctgccg atgccgccat
    12721 catgtccacc tacatttgca atctggtgca cttcgtttgg ggaaccgcga accgcgaacc
    12781 cttactgcaa aagtcctggc gcgaccggct gcacggatac atcggcggca tacttgagaa
    12841 taaacgcgct aagctactgg ccgccggcgg catcgccgat cacattcacg ttctcgcctc
    12901 gctcccggcc acgctctccc tggcggatgc cgccaatgcg atgaaagcca actccagccg
    12961 ctggattcat gagaacgcgc cgcagtccaa gggatttgac tggcaggagg gttatggcgc
    13021 gttttccgtg agtaagt
//
LOCUS       DASRJB010000046        10739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1990901, whole genome shotgun sequence.
ACCESSION   DASRJB010000046 DASRJB010000000
VERSION     DASRJB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990901"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFB80_01330"
     CDS             <1..1294
                     /locus_tag="VFB80_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32421.1"
                     /translation="NYGNFSGTNANEQGLAPPLRMRWARRLEGTVKHLPVCGGGRLYT
                     HTAEGQIIAVEQDTGRLLWRRYWPDVYLSFTSPLYHNEKLLIPQAGIKQSKMRCLDAA
                     TGKLVWESPFTGSPSWSRQFPPVVHGNVAIYASGSGQYAFQGTEKPYTFKGTPQPAEG
                     GAEVMAFIYSNDNPYYPKDNRPLLWAWDLNSGKVVWEKDFSDQGRGGNDCGICLLDGK
                     LYYSTFFGYSASQRVRRGLPPENNGLTACLDPQTGDVIWQTNKYFVTAKCTLSAKDGR
                     LYIGGYNPAREETKDRFVWCLDAKDGSLIWQSDPVTSALNVVTIGDRFIFSNALRGRG
                     NVFDKETGKVVGGVDHNYACCRFTMSGSYVLGANMDMIDLAAGGKLVSTGPAIDSREC
                     LGAVVSNGRIFYTSQASGFVVSQTFGEASKNLPAVWER"
     gene            1347..2066
                     /locus_tag="VFB80_01335"
     CDS             1347..2066
                     /locus_tag="VFB80_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32422.1"
                     /translation="MSFFTSLIFYRPRTPPLLTANDLAQFVARLRDTTLLVDGGLRSL
                     KIKFGDAVDQDDKSTTWNEPLGGGRLFVTREIEWDQHLSDPTIAEMVECLTGDGRRIY
                     RAWISFGALSDEVLQPITRLNSPENDRDFCPDGLSLEVGPIEIYSLGTELIAHVGWIG
                     LSISGSGYLFPWTIQEVVERAATAPAIQRLTDVCRASWPVTPERPEPRIAEARKQLGE
                     LWPYADLHKTWDWYWGVCETG"
     gene            complement(2096..2629)
                     /locus_tag="VFB80_01340"
     CDS             complement(2096..2629)
                     /locus_tag="VFB80_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN32423.1"
                     /translation="MHWFVFLTLAVCGCQSRTAPAPAAAVNAKLASWAETEQLIASHK
                     GKVVVLDVWSTWCAPCVAEFPGLAKLHQQYPGKVACISLNANYTGGDEPPGADREQIE
                     AFLAKSRATFDNLICTDPDERLYQSLEAAAVPVVRVYDRTGQLRKQFDNDENEFGDEG
                     FSYERHVGPLVEKLLKE"
     gene            complement(2664..4508)
                     /locus_tag="VFB80_01345"
     CDS             complement(2664..4508)
                     /locus_tag="VFB80_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32424.1"
                     /translation="MRTLAAWIAMCCLGGAAVQAGDSNSLLDISRDAKLLACANRDSG
                     TVTIVELATHAKRAEIAVGRHPEGVSFVGDSHQLAVALYDDDCVAILDAESGAIVRRI
                     DVFDEPYGIVSSADGRRLFVTLDYPGQVLEIDPAAGTIVRTLEAGDFARGIAIAPDQS
                     RLLVTQYYSGEVLAIDLPGGKIADRWPGISNDNLARQIAVHPKRGKAYLPHIRSRVTA
                     VHGEGSIFPYVSVIDTVSKDTTAGDGQRRRRIPMDAFLANLVTANPWEAAISADGRQF
                     YVVFAGTDDMFACDVIDDDYREIGYRKLVRLGRNPRAVRIAPDGQTLYVYNALDFNVV
                     AYDTRSLTPQTTIAVTENPLGEEKLRGKVLFYSAQQPMVGRRWISCSSCHPDGQQDGR
                     TWHNPEGLRNTQWLAGMAWTHPIHWSADRDEVQDFEHTIRGPLMQGRGLIRGAVQKEL
                     GTPNKGLSADLDALAAYSNSHTWPLSPYAKQGLSAAARRGQAIFISAETLCATCHSGP
                     LFTDSSVKSMAMHDVGTGADDASEKLGPKFDTPTLLGVYRTAPHLHHGQAATLRDVLT
                     TKNAGDRHGKTSHLSSGQIDDLVEYLKALPYEDPQPAAAAAGLTKVDR"
     gene            complement(4599..4964)
                     /locus_tag="VFB80_01350"
     CDS             complement(4599..4964)
                     /locus_tag="VFB80_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32425.1"
                     /translation="MSIFAREITDDLASLVKQVDEQVGKNSSKQMKAFVVLLAEDADA
                     AAPKLEELAKKQGIKNVPLTIFDGASGPPSYKIAKDADVTVLLWRGQTVKANHAFGKG
                     GLDKKGTEAIIADAPKILE"
     gene            complement(4986..5150)
                     /locus_tag="VFB80_01355"
     CDS             complement(4986..5150)
                     /locus_tag="VFB80_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32426.1"
                     /translation="MRRIWMPSIALAVAAAGVVIAADVQSGLKVGEPAGAFNVKDITG
                     PNKGKSLCYR"
     gene            complement(5273..5749)
                     /locus_tag="VFB80_01360"
     CDS             complement(5273..5749)
                     /locus_tag="VFB80_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN32427.1"
                     /translation="MRTGLAVALLMAVPLIRIARCEEHAQKISLHPPDQLKWQDGPPS
                     LPKGAKIAVLEGDPNKEGPFVFRVKAPDGYRIPPHTHPKMERVTVISGTFHIGMGDKF
                     DEQAAEAMPAGTYGYWPPGMKHFVWVKGETVVQFHGLGPWSIQYVNPADDPRNQPK"
     gene            complement(5778..6764)
                     /locus_tag="VFB80_01365"
     CDS             complement(5778..6764)
                     /locus_tag="VFB80_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZN32428.1"
                     /translation="MSQQRTSAVAIVLLFAGIPAMAAEPASSPHQRQFRLTYAVTVTG
                     LEAGQAARLWMPVPPDNDEQRVQVVEQSLPGASQQAREPKYGNDILYVAAAADRAGQV
                     TARTVYGVTREEARGTPAVMMKDNELAALFLKPDARVPVGGKPLTLLAGKSLPMDQMR
                     LGRVLYDVVNGHMRYSKEGTGWGLGDAVWACDSKYGNCSDFHSLFISLARSQGVPAKF
                     EIGFPLPPARGRGEIAGYHCWAWFQPRGFGWVPVDISEANKNPELREYYFGNLTENRV
                     TLSTGRDLVLAPPQAGPPLNFFVTPYVEVAGKPYPAEKVQLKLQYEDVEGRP"
     gene            complement(6784..9681)
                     /locus_tag="VFB80_01370"
     CDS             complement(6784..9681)
                     /locus_tag="VFB80_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN32429.1"
                     /translation="MTAEKARLLSSAVIFAGCLFFGLAAEGQNPPARQPWTTSRVQGS
                     PVAPEPFRLAPAFANLRFQLPTSVEESPSGRLLVTQRDGKIFSFPNQADVRSADLVAD
                     LAAGLTGELAAKGVSLFDAELHPKFVENRFLFVCYVHPDGGGQTRLSRLTLTADDAPR
                     VAPGSEKVVLTWPSGGHNAGCLEFGTDGFLYIATGDGTGPNPPDGLTTGQTVTDLLGA
                     ILRIDVDRTSGELAYVVPADNPFVEMKGARPEIWAYGLRNPWKFGVDPKTGNIFAADN
                     GWESWEMVHQIVRGGNCGWPVMEGRAALRTEVPVGPTPIRPPVMDHSHTEANSVIGGP
                     VYRGNKLADLDGSFVYGDYITGTIWAIRPNPAGGYGVRTLVDTDQRIVAFTQTSRGQL
                     LVLDYDFTGQIYELLPSDVPDTSATFPRRLSETGLFASTAKLEPAAGVVPYRVKVAPW
                     NDGAAAQRWVAIPGTGQIELSAGEQPASYPEGTVLVKHLSLPQASGDSIRLETQLLHL
                     ERGTWRPYSYLWDEAGQDAQLVDPAGASRPLKPAGLAERTWRVNATNECKLCHNAGPR
                     FVLGFVPNQLNMTPLVAAGVFAKPAQPPADDPLRLVDPHDPTQPLEDRARSYLHVNCA
                     MCHHPGGNAIVSFFLRRDLPFEKLNTNKGTGIGTFGMPEAKIIAAGDPYRSVLLYRMA
                     KLGYARMPYIGSQVVDSDGVLLIEKWIRSLPAEAGGSVSTPLAANSQEAGALKLVTAD
                     RRTDAMFDTAVQTLLKSTPASLALIARLHGSNLSEQGAQSAIGYGSAVNSADVRGLFD
                     TFLPENKRRVTLGASPDPQKILSLTGDRARGQLIFASDGARCRNCHDASDRGQSIGPT
                     LQEINKKYAKRDELLQHVLQPSLKVEDAFAAHAALTNDGRTVTGLVAEQSDQEVVLKT
                     AEKQLVRIRRAEIAELRKSPKSLMPDQILSDLTAQQAADLLEYLRSLGSGP"
     gene            complement(10023..>10739)
                     /locus_tag="VFB80_01375"
     CDS             complement(10023..>10739)
                     /locus_tag="VFB80_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32430.1"
                     /translation="ILRPLRGARNYPRRFATTYAVETEPGIHALVTRLSATSHLSRPP
                     QDPRPATLYVSHHSADVELRSEPLIKELLTDEDAPFFACDLRGIGESRPDTCGSDQFL
                     NPYGSDYFYSSYSLMLGRPYVGQKTLDLLRVLDWLASLGHREVHLAARGWGAIPAAYA
                     AVLRPAVTQVTLKQGLASYAAIAESENYNWPLSALTPGILARFDLPDCYRELDQTRKL
                     QQLDPQGAVASIVERASQAK"
BASE COUNT         1856 a         3495 c         3413 g         1975 t
ORIGIN      
        1 gaactacggc aacttctccg gtaccaacgc caacgaacag gggctcgccc cgccgctgcg
       61 gatgcggtgg gcccgccgcc tggaaggtac cgtcaagcac ctgcccgtct gcggcggcgg
      121 ccggctctac acgcacaccg ccgagggcca gatcatcgcc gtcgagcaag acaccggccg
      181 tctcctttgg cggcgctatt ggcccgacgt ctatctctcg ttcacctcgc cgctctacca
      241 caacgagaaa ttgctcattc cgcaggccgg catcaagcag tcgaagatgc gctgcctcga
      301 cgccgccacc ggcaagctgg tctgggagtc gcccttcacc ggctcgccaa gctggagccg
      361 gcagttcccg ccggtcgtac acggcaacgt cgcaatctat gcctccggct ccggccagta
      421 tgccttccaa ggcacggaga aaccctacac gttcaagggc acgccgcagc cggcggaggg
      481 cggggccgag gtcatggcct tcatctactc gaacgacaac ccgtattatc cgaaggacaa
      541 ccggccgctg ctctgggcct gggacctgaa tagcggcaag gtcgtttggg aaaaggattt
      601 ctcggatcag ggccgcggcg gcaacgactg cggcatctgc cttttggacg gcaagctcta
      661 ctactcgacc ttcttcggct actcggccag ccagcgcgtc cgccgcggcc tgccgccgga
      721 aaataacggc ttgaccgcct gcctcgatcc gcagaccggc gatgtgatct ggcagacgaa
      781 caagtacttc gtcacggcca agtgcacgct ctcggcgaag gacggccggc tctatatcgg
      841 cggctacaac ccggcccgcg aggagaccaa ggaccgcttc gtgtggtgcc tcgacgctaa
      901 ggacggctcg ctgatctggc agtccgaccc agtcacctcc gccctgaacg tcgtcacgat
      961 cggcgaccgg ttcatcttca gcaacgccct ccgcggccgt gggaacgtct tcgacaaaga
     1021 gacagggaag gtggtcggcg gcgtcgatca taactatgcc tgctgccggt tcacgatgtc
     1081 cggctcgtac gtgctcggcg cgaacatgga catgatcgac ctggccgccg gcggcaagct
     1141 ggtctcgacc ggcccggcaa tcgactcgcg cgagtgcctg ggcgccgtcg tttccaacgg
     1201 ccggatcttc tacacgtcgc aggccagtgg cttcgtcgtc tcgcagacct tcggcgaggc
     1261 gtcgaaaaac ctgcccgcgg tttgggagcg gtagcatggc tatgcgcact gaaattgcct
     1321 acgagggatc ttgatggccc ggacccatgt cgttcttcac gagcctcatc ttttatcgac
     1381 ccaggactcc accgctgctc accgcaaatg atctcgcgca gtttgtggca cggctccgcg
     1441 acacgactct gctagtggat ggcggcttac ggtcattgaa aatcaagttc ggagacgccg
     1501 ttgaccagga tgacaagtcc acgacgtgga atgaaccgct aggagggggt cgcctgtttg
     1561 ttacgcgtga aattgaatgg gatcaacacc tctcagatcc gacgattgcc gaaatggtgg
     1621 agtgcctaac cggcgatgga cgccgcatct atcgagcgtg gatctcgttc ggggctctga
     1681 gtgacgaagt gctgcagccg attactcgct tgaattctcc agagaacgac cgagacttct
     1741 gccccgatgg cttatcgctg gaggtgggcc cgatcgagat ttacagcctt gggacggaac
     1801 tcatcgcgca cgtcggctgg atcggtctga gtatcagcgg ctccggttat ctatttcctt
     1861 ggacgattca ggaggtcgtc gagcgcgccg caaccgctcc ggcgattcag cgacttaccg
     1921 atgtttgtcg cgcgtcctgg ccggtaactc ctgagcgacc cgagccgcgc attgccgagg
     1981 cacgcaagca acttggagaa ctgtggccgt atgccgacct ccacaaaacg tgggattggt
     2041 actggggtgt gtgcgagacc ggatgactcg cttcgccggc ttgagctgcc tgcggctatt
     2101 ctttgagcag cttctcgacg agcggtccga catgccgctc gtaggagaat ccttcgtcgc
     2161 caaactcgtt ctcgtcgttg tcgaattgct ttcgaagctg gcccgtgcgg tcgtagacgc
     2221 ggacgaccgg caccgcggcg gcttcgagtg actgatagag ccgctcgtcg ggatcggtgc
     2281 agatcaggtt gtcgaacgtt gctctcgact tggccaagaa cgcctcaatt tgctcccggt
     2341 cggcgcccgg cggctcgtcg cccccggtgt agttggcatt gagcgagata caggcgactt
     2401 tccccggata ctgctggtgg agcttggcca gcccgggaaa ctcggccacg caaggtgcgc
     2461 accaggtgga ccagacgtcg agcacgacga ccttgccctt gtggctggcg atcagctgct
     2521 ccgtctccgc ccacgaggct aatttcgcgt tgactgccgc ggccggcgcc ggcgcggtcc
     2581 gcgactggca gccgcaaacg gcaagcgtca ggaaaacgaa ccaatgcata tgccggaaca
     2641 taaccatcca cgaaatgaga acgtcagcgg tcaaccttcg tcaggcccgc ggctgccgcg
     2701 gccggctgcg gatcttcgta ggggagcgct ttgaggtact ccacgaggtc gtcgatctga
     2761 cccgatgaca ggtgactggt cttcccatgc cgatcgcctg catttttggt cgtcagcacg
     2821 tcgcgtaagg tcgccgcctg gccgtggtgc aagtgcggcg cagtgcgata gacgcccaac
     2881 aaagtcggag tatcgaattt cggcccgagc ttttcgctgg cgtcatccgc gcccgtgccc
     2941 acgtcgtgca tggccatcga cttgaccgac gaatcggtga agagcggccc gctgtggcac
     3001 gtcgcgcaca gcgtctcggc agaaatgaag attgcctggc ctcgccgggc ggcggcgctc
     3061 aggccctgct tggcgtacgg actgagcggc cacgtgtgcg agttcgagta ggcagccagc
     3121 gcgtcgaggt cggcggagag tcctttgttg ggcgtcccca gctccttctg caccgcgccg
     3181 cgaatcagcc cgcggccctg catcagcggt ccgcggatcg tgtgctcaaa gtcctgcacc
     3241 tcgtcgcgat cggccgacca gtggatcggg tgcgtccacg ccatcccagc cagccactgc
     3301 gtgtttcgca gcccctcggg attgtgccag gtccgcccgt cctgctgccc gtcggggtgg
     3361 cagctcgagc agctgatcca gcgccggccg accatcggct gctgggccga atagaacagg
     3421 acttttccgc ggagcttttc ttcgcccagc ggattttcgg tgacggcgat cgtcgtctgc
     3481 ggggttagcg atcgcgtgtc gtaggccact acgttgaaat cgagggcgtt atagacgtac
     3541 agagtctggc cgtccggcgc gattcgcacc gcccgcggat tgcgccccag ccgcaccagc
     3601 ttgcgatagc cgatctcgcg atagtcgtcg tcgatcacgt cgcaggcgaa catgtcgtcg
     3661 gtgccggcga acacgacata gaactgccgc ccgtcggccg agatcgccgc ctcccacgga
     3721 ttggcggtca ccagattggc caggaaggcg tccatcggaa tccgccgccg ccgctgccca
     3781 tcgcccgcgg tagtgtcttt cgaaacagtg tcgatgaccg atacgtacgg aaagatcgat
     3841 ccctcgccat gcacggcggt cacgcgcgag cgaatgtgcg gcagataggc cttgccgcgc
     3901 ttgggatgca cggcgatctg ccgcgcgagg ttgtcgttgc tgatccccgg ccagcggtcg
     3961 gcaattttgc cgccgggcag atcgatcgcc agcacctcgc cgctgtagta ctgcgtcacc
     4021 agcaagcgcg actgatcggg cgcgattgca atgccgcggg caaagtcgcc ggcctccagc
     4081 gtccgcacga tcgttcccgc ggccgggtcg atctccagga cctggcccgg atagtcgagc
     4141 gtgacgaaca gcctccggcc gtcggcgcta ctcacgattc cgtagggctc gtcgaagacg
     4201 tcgatccgcc gcacgatcgc gccgctctcg gcatcgagaa tcgcaacgca gtcgtcgtcg
     4261 tacagggcga cggcgagctg gtggctgtcg ccgacaaagc tgaccccttc gggatgcctg
     4321 ccgacggcaa tctccgcccg cttcgcgtgc gtcgccagct cgacgatcgt caccgtgccg
     4381 ctgtcgcggt tggcgcaggc cagcagcttc gcgtcgcgcg aaatatccag caggctgttg
     4441 ctgtcgccgg cctggacggc agcgccgccg agacagcaca tggcgatcca ggccgctaac
     4501 gtgcgcatgg atgtgtttgc aagagaaatg agaaaggccc gccccgcgaa agtgcgggac
     4561 gggccgggtg cttgcgcgga attgggtgtt acgcgaaact actcgaggat cttcggcgcg
     4621 tcggcgatga tcgcctcggt gcccttcttg tcgagcccgc ccttgccaaa ggcgtggtta
     4681 gccttgacgg tctgcccccg ccacagaagc acggtcacgt ccgcgtcctt ggcgatcttg
     4741 tagctcggcg ggccggaagc gccgtcaaag atggtcagcg ggacattctt gatgccctgc
     4801 ttcttggcga gctcctcgag cttgggagcc gcagcgtcgg cgtcctcggc gaggagcacg
     4861 acgaaggcct tcatctgctt gctgctgttc tttccaacct gctcatccac ttgcttgacc
     4921 aaactggcca agtcgtccgt gatctcgcga gcaaaaatgc tcacgaccgg acggtctgca
     4981 tacgctcatc gatagcagag cgatttgccc ttgttcgggc cggtaatgtc cttcacgttg
     5041 aacgccccgg caggctcgcc gaccttgagc cctgattgca catcggctgc aatcacgacc
     5101 ccggctgcgg cgaccgccaa ggcgatcgac ggcatccaaa ttcgtcgcat cgactggctc
     5161 ctatgaaatg aaaaacctgg aaacgcgcaa cacccaatac ttatctactg tatcggctct
     5221 caagccgcaa ccgcgagacc cactatcttg gcctctttca ggctgctgcg gctcattttg
     5281 gctgatttct gggatcgtcg gccggattga cgtattgaat cgaccacggc cccaagccgt
     5341 gaaattgcac gaccgtctcg cccttgaccc aaacgaagtg cttcatgccc ggaggccagt
     5401 agccgtaagt tccggcgggc atcgcctcgg cggcctgctc gtcgaacttg tcgcccatgc
     5461 cgatgtgaaa cgtaccggag atgacggtca cgcgctccat cttgggatgc gtatgtggcg
     5521 ggatgcggta gccgtcgggc gccttgacgc gaaacacgaa gggcccttcc ttgttcgggt
     5581 ctccttcgag cacggcaatc ttcgctccct tgggcaacga cggcgggccg tcctgccact
     5641 tgagctgatc cggcggatga agcgagatct tctgggcatg ctcttcgcag cgcgcgatcc
     5701 gaatcagcgg cactgccatc agcagcgcca cagcgaggcc agtgcgcatc tctgtttctc
     5761 ccaaaaagtg aagcgaacta cggacgcccc tccacgtctt cgtattgcag cttgagttgc
     5821 actttctcgg ccgggtatgg cttgcccgcg acttctacat acggcgtcac gaagaagttg
     5881 agtggcgggc cggcctgcgg cggcgcgagc accaggtcgc ggccggtgct gagcgtcacc
     5941 cggttctccg tgaggttgcc gaaatagtat tcgcgcagct ccgggttctt gttggcctcg
     6001 gagatatcga ccggcaccca gccgaagccc cgcggctgaa accaggccca gcagtgatag
     6061 ccggcgatct ccccgcggcc ccgcgcgggc ggcagcggaa agccgatctc gaacttggcg
     6121 gggacgccct gcgagcgggc cagcgagatg aacaggctgt ggaagtcgga gcagttgccg
     6181 tacttgctgt cgcaggccca gaccgcgtcg cccaggcccc agccggtacc ttccttgctg
     6241 taccgcatgt ggccgttgac cacgtcgtac agcacgcggc ccagccgcat ctgatccatc
     6301 ggcaggctct tgccggccag cagcgtcagc ggcttcccgc ccacgggcac gcgggcgtca
     6361 ggcttgagaa acagtgcggc tagttcgttg tccttcatca tgactgccgg cgtgccgcgg
     6421 gcttcctcgc gagtcacccc atacaccgtg cgggcggtga cctggcccgc gcgatcagcc
     6481 gccgccgcga cgtacagaat gtcgttgccg tatttcggct cgcgggcctg ctgggacgct
     6541 cccggcagcg attgctccac gacctgcacg cgctgctcgt cgttgtccgg cggcaccggc
     6601 atccacagtc gcgccgcctg gccggcctcc aggcccgtga ccgtcacggc atacgtgagg
     6661 cggaactgcc gctgatgcgg cgacgatgct ggctcggcag ccatggcggg gatgccggcg
     6721 aatagcagga caatcgcaac ggcgctggtt ctctgctggc tcatcgttgg tcacctgatg
     6781 gaattagggg ccactcccca gcgatctaag atactctaac aagtcggctg cctgctgggc
     6841 cgtcaggtcg ctgagaatct ggtcgggcat cagcgacttg ggactcttcc gcaattcggc
     6901 gatctcggcg cggcggatcc ggacgagctg cttctcggcg gtcttgagga ccacttcctg
     6961 gtccgattgc tcggctacca gtcccgtcac cgtgcggccg tcgttggtca gggccgcatg
     7021 agccgcgaag gcgtcttcga ccttcagtga cggctgcagc acatgctgaa gcagctcgtc
     7081 ccgcttcgcg tacttcttgt tgatctcctg aagcgtcgga ccgatcgatt gcccgcggtc
     7141 ggacgcgtcg tggcaattgc ggcagcgagc gccgtcgctc gcgaaaatca gctggccccg
     7201 cgcgcggtcg ccggtcaggc tgaggatctt ttgcggatcg ggactggcgc cgagcgtcac
     7261 ccgccgcttg ttttcgggaa gaaacgtgtc aaacaggccg cggacgtccg cggagttgac
     7321 cgccgacccg tagccgatgg ccgactgagc tccctgctcg gacaaattgc tcccgtgcag
     7381 cctggcgatc aacgccagcg acgcaggcgt ggatttgagc agcgtctgca ctgccgtgtc
     7441 gaacatcgca tcggtccggc gatcggcggt aaccagcttg agcgcgccgg cttcctggga
     7501 atttgccgcc agcggcgtcg aaacgcttcc gcccgcttcc gccggcagcg agcgaatcca
     7561 cttctcgatc agcaagacgc cgtcgctatc gacgacctgc gagccgatat agggcatccg
     7621 cgcgtagccg agcttcgcca tccgatacag cagcacgctg cggtaaggat cgccggccgc
     7681 gatgattttg gcctcgggca tgccgaacgt tccgatgccg gttcccttgt tcgtgttcag
     7741 cttctcgaac ggcagatcgc gccgcaggaa gaacgacacg atcgcgttgc cgcccggatg
     7801 gtggcacatc gcacagttca cgtgcaggta cgaccgggcc cggtcctcga gcggctgcgt
     7861 tggatcgtgc ggatcgacca gccgcagcgg atcgtcggcc ggcggctgcg ctggcttcgc
     7921 gaatacgccg gctgcgacga gcggcgtcat gttcagctga ttgggcacga agcccagcac
     7981 gaaccgcggc ccggcgttgt ggcagagctt gcattcgttg gtcgcgttca cgcgccacgt
     8041 ccgctcggcc aggccagccg gcttcagcgg ccgacttgcg ccggccggat cgaccagctg
     8101 cgcatcttgg cccgcttcgt cccacaaata gctgtacggc cgccaggtgc cgcgctcgag
     8161 gtgcaagagc tgcgtctcta gccggatgga atcgccgctg gcctgcggca gcgataagtg
     8221 cttcaccagc accgtccctt ccgggtagct cgccggctgc tcgcccgcgg acaattcgat
     8281 ctggccagtg ccaggaatcg ccacccagcg ctgcgccgct gcgccgtcat tccacggggc
     8341 gaccttcacg cggtacggca cgacgcccgc cgcgggctcc agctttgcgg tcgaggcaaa
     8401 cagtcccgtc tcgctcaatc gccgcggaaa cgtggccgag gtgtcgggca cgtcggaggg
     8461 cagcagctcg taaatctgac ccgtgaagtc gtagtcgagc accagcagct ggccgcggct
     8521 ggtctgcgtg aaggccacga tccgctgatc ggtatcgacg agcgtccgca cgccgtagcc
     8581 gcccgcggga ttcggccgga tcgcccagat cgtgccggtg atgtagtcgc catagacgaa
     8641 cgagccgtcc aggtcggcga gcttattgcc gcgatagacc gggccaccga tcaccgagtt
     8701 ggcctcggtg tgcgaatggt ccatcaccgg cgggcggatt ggcgtcggcc cgacaggcac
     8761 ttccgtccgc agtgccgcgc ggccttccat cacgggccag ccgcagttgc caccgcgcac
     8821 gatctgatgg accatttccc aggattccca gccattgtcg gcggcgaaaa tattgccggt
     8881 cttgggatca acgccgaact tccacgggtt ccgcaggcca taggcccaaa tctccggtcg
     8941 cgcgcctttc atttccacga acggattgtc ggccggcacg acatacgcca actccccgct
     9001 agtgcgatcg acgtcgatcc gcaggatggc gccgagcagg tcggtcaccg tctgtcccgt
     9061 cgtcagcccg tccggcggat tcgggcccgt gccgtcgccg gtggctatgt agaggaagcc
     9121 gtccgtcccg aattccaagc agccggcgtt gtggccgcca gagggccagg tcaggacgac
     9181 tttctcgctg ccaggcgcca cccgcggtgc gtcgtccgcc gtgagcgtca gccgcgagag
     9241 gcgcgtttgc ccgccgccgt ccgggtggac gtagcagacg aagagaaacc gattctcgac
     9301 gaatttcgga tgcaattccg cgtcgaaaag cgaaactccc ttggcggcca attctccggt
     9361 caatccggcg gccagatcgg ccacgagatc ggctgaccgg acgtccgcct ggttcgggaa
     9421 gctgaaaatc ttgccgtcgc gctgggtcac cagcaggcgg ccgctcggag actcttcgac
     9481 gctggtcggc aactgaaacc gcaggttggc aaacgcgggg gcgaggcgaa aaggttccgg
     9541 cgcgaccggc gagccctgca cgcgggaggt cgtccaaggc tggcgggcag gcgggttctg
     9601 cccctcggcg gccagcccaa agaacagaca gccagcaaag atgaccgctg aactgagcag
     9661 tcgcgccttt tcggcggtca tcgccgcgac tcgctgaaca ggtcgcctcg aaaagtctga
     9721 acagtcccct tccgggcggt tttggctcgg agtgcagcgg cgacttcttg gaggcaattg
     9781 ttgcataagt cttttcccaa tatgtacttg cgtaacactc cctgcatgag agatagaagc
     9841 actagagact gcttccgtcg cggtctgaac agggcgcgcg cagcgcgaga atcgcaatcc
     9901 acgcgaatta cgagccacca acttgccaat gagcgagagg ttgcgccgac gcgcggagat
     9961 tcctccagac ctcatatgta tagatgcgca cgttgaggaa tttattgccc gcatcttcca
    10021 ctttattttg cttgactcgc tcgctccaca atgctcgcca ccgcgccttg cggatcgagc
    10081 tgctgaagtt tgcgggtctg gtccagttcg cggtagcagt cgggcaggtc gaaccgggcg
    10141 aggatgccag gcgtcagcgc ggagagcggc cagttgtagt tctcgctctc ggcgatcgcg
    10201 gcgtaggacg ccagcccctg cttgagcgtc acctgggtca cggccggccg gaggacggcg
    10261 gcgtaagccg cggggatcgc gccccagccc ctggcggcca ggtgcacctc gcggtggccc
    10321 agcgatgcga gccaatcgag cacgcggagc agatccaggg tcttctggcc gacgtacggc
    10381 cgacctagca tcagcgaata cgacgagtag aagtagtcgc tgccgtaggg attgaggaac
    10441 tgatcgctgc cgcaggtgtc gggccgtgat tcgccgatgc cccgcaggtc gcaggcaaag
    10501 aacggcgcgt cctcgtcggt caacaactcc ttgatcagcg gctcgctgcg gagctccacg
    10561 tcggccgaat ggtgcgacac atagagcgtc gccggccgcg gatcttgcgg cgggcgggag
    10621 aggtgcgacg tggcgctcaa gcgcgtcacc agcgcgtgaa tgcccggctc ggtctcgacg
    10681 gcgtacgtcg tggcaaagcg gcggggataa tttcgggccc cccgcagcgg acggagaat
//
LOCUS       DASRJB010000047         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2556294, whole genome shotgun sequence.
ACCESSION   DASRJB010000047 DASRJB010000000
VERSION     DASRJB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556294"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3545
                     /locus_tag="VFB80_01380"
     CDS             <1..>3545
                     /locus_tag="VFB80_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZN32431.1"
                     /translation="DGGIVECPLKHTDVQADIAGFVARVRVTQTFHNPTKEKVEAVYV
                     FPLPHESAVDEMTMVLGDRKIVGLIKRRMEARQIYEAALRAGQTAALLEQERPNIFTQ
                     SVGNIEPGQEVKIEIGYVDVLRYDMGTYEFHFPMVVGPRYIPGTPSGGAPQQPANLQG
                     KVSPPVADTDRVPDASKISPPVLKPGVRNGHDISLSVKLDAGVPIQNLGSNSHDAGID
                     RRGDRQATIKLSPADSIPNKDFVLRYGVIGKKPEMAVLGHTGNYSGDSQKLGNGYFML
                     MIQPKEDERLTKSPPREIVFLVDVSGSMSGEPTNKVIQAMQGMLKLCREKDTIQVITF
                     ASQANKLFDKPVPVNDENIGKALGFTSGLRGSGGTEMLKGVQMAIDEPIDKERLRIVV
                     MLTDGYIGNEAEIIEHVGKHCGDQIRFWCVGIGSAPNMFLVDGVAKQGGGMGKKLNLP
                     DDAQPLVQEIMTRIQRAQLAKLQIDWGDLKVSETYPAKLPELWAGRPVIVYGRYSGGA
                     AKDGSFQSQITVKGNVEGEAVSWPLTVALPQEQKDHDALAKVWARQKIEDLMHQTYYQ
                     GSPAVEESVTAIALDYKLMSQYTSFVAVDSSKAPDESAAATPPRRMLVPVPLPAGTQW
                     EGFFGPEGDAEEVAALAIRFDRFALKKEQESLKEAKASLSVARRPLGESTPRLRASRF
                     FAPTSGPMPAARTPDGGGFGGVSAGLGFAPAGGPGFGRGGFRAGKQVAQGIDGRLGRG
                     RGLSNYFENGKAGEAKGRGWGLDGLTRLHDSLGDVAERLKDVSDGEFYYAASALAPQG
                     GKLAEAARAAWGAANNKEKPVQGDALRRLIAKAVLLDAAAANLGQSDGSIGSQALEKL
                     SELRQADIKAWSEKQPLLNTKLDLVLRDQSLEQALAAVAKAAKIDIRLLEGSAADAAA
                     LTGTSPRISYLDLRHATVAQALDWILQPERLSWWPDVARDSVAGGPPRSGGLQIVAGS
                     ERRRGEAGWTYDVSAIALPLEEDLKKLGEEAKMVAEMQKAAEEFIKAVRGRLKVDEAS
                     LVWFAPGHLLLFGTPEQHEIMAGLIPALQSATQRPRELPVALAEVTRQRYAARKERLE
                     KAAAARRTLDVAMSHDHFSWQLLSAAAGGKLDLEALTELQIAWKATQTAQLLAGQSRP
                     LMLRSLWTVCEASRALPAEKELAALATAARAAAAK"
BASE COUNT          674 a         1160 c         1209 g          502 t
ORIGIN      
        1 gatggcggca tcgtcgaatg cccgctgaag cacacggatg tgcaagccga catcgccggc
       61 ttcgtggccc gcgtccgcgt gacgcagacc ttccacaacc cgaccaagga gaaggtcgag
      121 gccgtctatg tctttccgct gccgcacgag tcggccgtcg atgaaatgac gatggtcctg
      181 ggagatcgaa agatcgtggg cctgatcaag cggcgaatgg aagcccggca gatctatgaa
      241 gcggccttgc gggccggcca gacggcggcc cttttggaac aggagcggcc gaacatcttc
      301 acgcagtcgg tcggcaacat cgagcccggc caggaagtga agatcgagat cggctacgtc
      361 gatgtgctcc gctacgacat gggcacctac gaatttcact tcccgatggt cgtcggcccg
      421 cgctacattc ccggcacgcc cagcggcggc gctccgcagc agccggcgaa cttgcagggc
      481 aaggtctcgc cgccggtcgc cgacaccgac cgcgtgcccg acgccagcaa gatcagcccg
      541 ccggtcctga agccgggcgt ccgcaatggc cacgatattt cgctcagcgt aaagctcgac
      601 gccggcgtgc cgatccagaa cctcggcagc aacagccacg acgcgggcat cgaccgccgc
      661 ggcgaccgcc aggcgacgat caagctctcg ccggccgact cgatcccgaa caaggacttt
      721 gtcctccgct acggcgtgat cggcaaaaag cccgaaatgg ccgtcctggg ccacaccggc
      781 aactactcgg gcgacagcca gaagctcggc aacggctact tcatgctcat gatccagccc
      841 aaggaagacg aacggctgac caagagcccg ccccgcgaga tcgtgttcct ggtcgatgtc
      901 agcgggtcga tgtcgggcga gccgacgaac aaagtgattc aggcgatgca gggcatgctg
      961 aagctctgcc gcgaaaagga cacgattcaa gtcatcacct tcgccagcca ggcgaacaag
     1021 ctgttcgaca aaccggtgcc cgtcaacgac gagaacatcg gcaaggccct gggcttcacc
     1081 agcggcctcc gcggcagcgg cggcaccgaa atgctcaaag gcgtgcagat ggccatcgac
     1141 gagccgatcg acaaggagcg gctgcggatc gtcgtcatgc tcaccgacgg ctacatcggc
     1201 aacgaagccg agatcatcga gcacgtcggc aagcactgcg gcgaccagat tcgcttctgg
     1261 tgcgtcggca tcggctctgc gccgaacatg ttcctggtcg atggcgtcgc caagcaaggc
     1321 ggcggcatgg gcaagaagct caacctgccc gacgacgccc agccgctggt gcaggaaatc
     1381 atgacccgca ttcagcgggc gcagctggcg aagctgcaaa tcgattgggg cgacttgaag
     1441 gtgagcgaga cctatccggc aaaactgccc gaattgtggg ccggccggcc ggtcatcgtt
     1501 tacggccgct acagcggcgg cgcggcgaaa gacggctcgt tccagtcgca gatcaccgtc
     1561 aaaggcaacg tcgaaggtga agctgtgagc tggccgctga cggtcgctct gccgcaggag
     1621 cagaaggacc acgacgccct ggccaaggtc tgggcccggc agaagatcga agacctgatg
     1681 caccaaacgt attaccaggg atcgcccgcc gttgaagaat cggtcacggc gatcgccctc
     1741 gattacaagc tgatgagcca gtacaccagc ttcgtcgccg tcgatagcag caaggccccc
     1801 gacgagagcg cggccgccac gccgccgcgg cggatgctgg tgcccgtgcc gctgcccgcg
     1861 ggcacgcagt gggaaggctt cttcggcccg gagggcgatg cggaggaggt cgcggcactc
     1921 gcaatccgct tcgatcgctt cgccctcaag aaggagcagg aatcgctcaa ggaagcgaag
     1981 gcatcgctca gcgtggccag gcgtccacta ggtgaaagca cgccgcgctt gagggccagc
     2041 aggttctttg cgcccacgtc cgggcccatg cctgcggcgc gcacccctga cggcggcgga
     2101 ttcggcggcg tcagtgccgg cttaggcttc gcgcccgccg gcggccccgg ctttggccgc
     2161 ggcggcttta gggcaggaaa gcaggtggcc cagggcatcg atggccgcct tggtcgcggg
     2221 cgcggtctct cgaactactt cgaaaacggc aaggcgggcg aggccaaggg ccgcggctgg
     2281 ggactcgacg gcctgacgcg actccacgat tcgctgggag acgtggccga gcgactaaag
     2341 gatgtgagcg acggcgagtt ctactatgcc gccagcgcgc tggccccgca gggcggcaag
     2401 ctggccgaag cggcccgcgc cgcttggggc gccgccaata acaaagagaa gcccgtccag
     2461 ggcgatgccc tccggcggct gatcgccaag gctgtgttgc tcgacgcggc cgcggccaat
     2521 ctaggccagt ccgacggcag catcggctcg caggccctgg agaagctgag cgaactgcgc
     2581 caagccgaca tcaaggcctg gagcgagaag cagccgctgc tgaacacaaa gctcgacctg
     2641 gtcctccgcg accagtcgct ggaacaggcg ctggcggccg tcgccaaggc cgccaagatc
     2701 gacattcgcc tgctcgaagg cagcgcggcc gacgcggccg ccttgacggg cacctcgccg
     2761 cggatcagct atctcgattt gcggcacgcc accgtggccc aagcgctcga ctggatcctc
     2821 cagcccgagc ggctgagttg gtggccggat gtagcccgcg actccgtcgc gggtggcccg
     2881 ccacggagtg gcgggctaca gattgtcgcg ggcagcgagc gccgccgcgg cgaagccggc
     2941 tggacgtacg acgtttcggc gatcgctctg ccgctggaag aggatctgaa gaagctgggc
     3001 gaggaggcca agatggtggc cgagatgcag aaggcggctg aggagttcat caaggccgtg
     3061 cgcggccgcc tcaaggtcga tgaagcgtcg ctggtctggt tcgctcccgg ccacctgctg
     3121 ctgttcggca cgcccgagca gcacgagatc atggcgggac tgatcccggc gctgcagtcc
     3181 gcgacgcagc ggccgcgcga gctgccggtc gcgctggccg aggtaacccg ccagcggtac
     3241 gccgcccgca aggagcggct ggagaaggcg gcggcggccc ggcggacgct ggacgtcgcg
     3301 atgagccacg accatttcag ctggcagctg ctttcggctg cggctggcgg caagctcgac
     3361 ctggaagcgt tgaccgagct gcaaatcgcc tggaaagcga cgcaaaccgc ccagctgctg
     3421 gctggccagt cgcgtccgct tatgcttcgc tcgctgtgga cggtctgcga ggcttcgcgg
     3481 gccctgccgg ccgagaagga actggctgcg ctggcgacag cggcgcgcgc ggcggcggcc
     3541 aagga
//
LOCUS       DASRJB010000048         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3402549, whole genome shotgun sequence.
ACCESSION   DASRJB010000048 DASRJB010000000
VERSION     DASRJB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3402549"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFB80_01385"
     CDS             complement(<1..426)
                     /locus_tag="VFB80_01385"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZN32432.1"
                     /translation="MATILDQIVATKREEVARAKATVPEGELRARLRDAPSVQSFFEP
                     LAAGGAIKLIAEVKKASPSQGVIRADFDPVAIARTYEAHGATCLSVLTDGHYFQGSLD
                     YLRQVRQAVGIPVLRKDFILGTYQLLEARAAGADAVLLIA"
     gene            complement(466..894)
                     /locus_tag="VFB80_01390"
     CDS             complement(466..894)
                     /locus_tag="VFB80_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZN32433.1"
                     /translation="MIVSAKTEYACIAVLELAMRHGGGEPVRIREISAAHGIPSRFLV
                     QILLQLKSAGLVQSVRGAAGGYNLAREPDEITLLDVMTVIDSQVGHTASNATRSTGPI
                     RILQRTWKNVAEKERELLEGVTFADLAEQLKGAAEGMYHI"
     gene            complement(891..1703)
                     /locus_tag="VFB80_01395"
     CDS             complement(891..1703)
                     /locus_tag="VFB80_01395"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZN32434.1"
                     /translation="MRSPSAVAELQTELPLLRATSSPLAANPSDIAAGDSPLELTDEL
                     AAEIRIASEKLENAEPVEILKWATDRFAPRFTMATAFGPEGMVLIHMLAEIAPQTPIF
                     NLDTGYQFNETLELREHVLRRYGIAVEMKRAETTVEQYERLHGGPLYRTNPDQCCLDR
                     KVKVLHQAVIGMHAWASAIRRDQSPDRAKAPIVGWDKKFNLVKVSPLANWTKQKVWGF
                     IAQHDIPYNPLHDQGYTSIGCWPCTRAVLLGEDERSGRWSGFAKTECGLHSQ"
     gene            1948..2808
                     /locus_tag="VFB80_01400"
     CDS             1948..2808
                     /locus_tag="VFB80_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN32435.1"
                     /translation="MSGQLSISFILSLLAASPAFGIDDYALGPDSMRQPEVPQGKVTE
                     HDWKTSKVYPGTERKYWIYVPAQYDGSKPASLMVFQDGEGFASETGAYRVPVVFDNLI
                     HKKEMPVTIAVMINPGVIPPSGDQSRPRRNRSYEYDRVTGDYATFLLDEVLPEVAKTY
                     KLDGDPNQRAICGNSSGGICAFSVAWHRPDAFRKVVSHIGSFTNIQGGHVYPALIRKT
                     ERKPLRVFLQDGSSDLDNMFGNWPLANQEMAAALKYSGYDYKFVYGDGGHTGKHGGSI
                     FPDTLRWLWR"
     gene            3345..>4028
                     /locus_tag="VFB80_01405"
     CDS             3345..>4028
                     /locus_tag="VFB80_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZN32436.1"
                     /translation="MGKRGKSQAAGRVGWETVAIVGVGLIGGSIGLALRERRLASRVV
                     GIGRRPASLAAAKKLGCVTETTTSLPRGVRRANLVVICTPVELIAGQVAEAGRHCPAG
                     CLITDAGSTKAALVSAAEAALDGRQPLPFVGSHPIAGSEKTGPEAARADLFVDRVVVV
                     TQSSASDKGVADAISDFWQSLGAQVIRMSPEAHDAALARTSHLPHLIASALAAATPDE
                     VLPLTAGGWL"
BASE COUNT          704 a         1357 c         1234 g          733 t
ORIGIN      
        1 ggcgatgagc agcacggcgt ccgcgccggc ggcgcgggcc tcgagcaatt gatacgtgcc
       61 caagatgaag tccttccgca ggaccggaat gccgaccgcc tgccgcacct gccgcaagta
      121 gtcgaggctg ccctgaaaat agtgcccgtc ggtcaggaca ctcaggcagg tggctccgtg
      181 cgcctcatag gtcctggcga tcgccaccgg gtcgaagtcg gcccgaatga ccccttggct
      241 ggggctggct ttcttcactt cggcgatcag cttgatcgcg ccgcccgcgg ccagcggctc
      301 aaagaagctt tgcacggatg gtgcatcgcg caaccgcgcc cgcagctccc cttccggcac
      361 agtcgccttg gcgcgcgcga cctcctcccg cttcgtcgca acaatctggt ccaaaatcgt
      421 agccacgcgc taccactcac cactcaccac tcacgcacca ctcacctaaa tatgatacat
      481 cccctccgcc gcgcccttca gttgctcggc caggtcggcg aacgtcacgc cttcgagcag
      541 ctcgcgctct ttctcggcga cgttcttcca ggtccgctgc aatatacgaa tcgggcccgt
      601 gctgcgggtg gcattgctgg cggtgtggcc cacctgcgaa tcgatgaccg tcatcacgtc
      661 cagcagcgtg atctcgtcag gctcgcgggc caggttgtat ccgcccgccg cgccgcggac
      721 gctctgcacc agccccgcgc tcttcagctg caacagaatc tgcaccagaa accgcgaggg
      781 aatgccgtgg gcggcagaga tttcgcgaat ccgcaccggc tcgccgccgc cgtgccgcat
      841 cgccaactcc agcacggcaa tgcaggcgta ttcggtcttg gcggagacga tcattggctg
      901 tgcagcccgc actcagtctt ggcaaaaccg ctccagcggc ccgatcgctc gtcctcgccc
      961 aggagcaccg cccgcgtgca cggccagcac ccgatgctgg tgtaaccctg gtcgtgcaag
     1021 gggttatacg ggatgtcgtg ctgcgcaata aagccccaga ccttctgttt cgtccagttg
     1081 gccagcgggc tgaccttcac caggttgaac ttcttgtccc agccgacgat cggggctttc
     1141 gcccggtcgg ggctctggtc acggcgaatc gcgctggccc aggcgtgcat gccgatcact
     1201 gcctggtgga gcaccttcac cttgcggtcg aggcagcact ggtccgggtt cgtccgatag
     1261 aggggcccgc cgtgcagccg ctcgtactgc tcgacggtcg tctcggcccg cttcatttcg
     1321 accgcgatgc catatcgccg caacacgtgc tcgcgcagct ccaacgtctc gttgaattgg
     1381 tatccggtgt cgagattgaa gatcggcgtt tggggcgcga tctccgccag catgtggatc
     1441 agcaccatcc cctcgggacc gaacgccgtc gccatcgtga accgcggggc aaaccggtcc
     1501 gtcgcccact tcaagatctc gaccggctcg gcgttctcca gcttctcgct ggcaatgcga
     1561 atctcagccg ccagctcgtc ggtgagctct agcggcgaat ctcccgccgc tatgtcagac
     1621 ggattggccg ctagcggcga cgaagttgcc cgtaacaggg gcagctccgt ttgtagttcg
     1681 gcgaccgctg atggcgaacg catggcagta aaccctctgg gacttccctc tcccagtgca
     1741 attctacggc gaatgttaca aatgttgcta accaaagtca acaatcgccg attcctccct
     1801 gtttcatcga ccgctggcgt tccgtctcta cagtttgtaa cggttagata agccgcggct
     1861 gcttgctact gccgcgctgc aacgttaccg taacattcga ctatcaaagc acttccggca
     1921 attgggcgac gatcttggag ccgcgtcatg tctgggcaac tttcgatttc cttcatcctc
     1981 agtttgcttg ccgcttcgcc tgcattcggg atcgacgact acgcacttgg ccccgactcg
     2041 atgcggcagc ctgaggttcc gcagggcaag gtcaccgaac acgactggaa aacgagcaaa
     2101 gtctatccgg ggaccgagcg gaagtattgg atctacgttc cggctcagta cgacggcagc
     2161 aaacccgcca gcctcatggt ctttcaggac ggggagggct ttgccagcga gacgggcgcc
     2221 taccgagttc cggtagtgtt cgacaacctg attcataaga aagaaatgcc ggtcacgatc
     2281 gccgtgatga tcaacccggg cgtcattccg ccgtcgggcg accagtcgcg gccgcggcgg
     2341 aaccgcagct acgagtacga tcgcgtgacg ggcgattacg ccacgtttct gctcgatgaa
     2401 gtcctaccgg aagtcgccaa gacctataag ctcgatggcg atccgaacca gcgggcgatc
     2461 tgcggcaaca gctcgggcgg catctgcgcc ttcagcgtcg cctggcaccg gcccgacgcc
     2521 ttccgcaagg tcgtcagcca catcggcagc ttcaccaaca tccagggcgg gcacgtctat
     2581 ccggccctca ttcgcaagac cgagcggaag ccgctccgcg tcttcctcca ggacggctcg
     2641 agcgacctgg acaacatgtt cggcaactgg ccgctggcca accaggagat ggccgccgcc
     2701 ctgaaatact ccggctacga ctacaagttc gtctatggcg acggcggcca caccgggaag
     2761 cacggcggct cgatctttcc cgacacgctt cggtggcttt ggcggtagga aggcgaggag
     2821 cgcaggatcc gacttttgcc gctgcgctgg gcgggggcgc ctcggcgggc acgtcgcgtg
     2881 aatcatttcg agactcccga gacagattgc aacccgaggc aaggcgaccg aatcgccgtc
     2941 ctttggcggc ccccggctgg gtgggctgcg cgcgcctctg tggccctcat tcttggcgca
     3001 acgcctgtcg cagaatcaac ttgtgtcggg gcgggcgaaa gacccataac tactacttaa
     3061 aacagttaga gaaagcctcc acgtgccgcc cgttgtgtgg cgcggcgcgg agatgcctcg
     3121 tcaaaaatgg caaccctcgc gccaaatgga acctacatgc aaatagcgta ccaatgttca
     3181 cagcgccaaa tcgccggtca ttaccagccg gcaccgcctc ccgccgccat gcgcggcctc
     3241 gaccagacca gcgcccccag ccgtagccga ttcgcgggct gtttgggcga agggccggtc
     3301 agcgcccttg gcatctgcca gttgtttgtg ttttgatgct ccgcatggga aagcgcggca
     3361 aaagccaggc tgccggccga gtgggctggg aaacggtcgc gattgtcggt gtcgggctga
     3421 tcggcggctc gatcgggctg gccctccgcg agcggcggct ggcatcgcgc gtcgtcggaa
     3481 tcggccggcg gccagcttcc ttggcagcgg ccaagaaact gggctgcgtc actgaaacga
     3541 cgaccagcct cccccgcggg gtcaggcggg cgaacctggt cgtgatttgc acgcccgtcg
     3601 aattgatcgc tggacaggtg gcggaggccg gccgccattg cccggccggg tgcctgatca
     3661 ccgatgccgg cagcaccaag gccgcgctag tgtcggcggc ggaggcggcg ttggacggcc
     3721 gccagccgct cccctttgtc ggcagccacc cgatcgcggg cagcgaaaag accggccccg
     3781 aagcggcgcg ggccgacttg ttcgtcgatc gtgtggtcgt ggtgacgcag tcgagcgctt
     3841 cggacaaggg cgtagcggac gcgatcagcg acttttggca gtcgctgggc gcccaggtca
     3901 tccgcatgtc gcccgaagcc cacgatgcgg ccctggcccg cacgagccac ctgccgcacc
     3961 tgatcgcctc ggccctggcg gctgccacgc ccgacgaagt gttgcccctg accgccggcg
     4021 gctggctc
//
LOCUS       DASRJB010000049         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3120831, whole genome shotgun sequence.
ACCESSION   DASRJB010000049 DASRJB010000000
VERSION     DASRJB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120831"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /gene="uvrB"
                     /locus_tag="VFB80_01410"
     CDS             complement(<1..1682)
                     /gene="uvrB"
                     /locus_tag="VFB80_01410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZN32437.1"
                     /translation="MTFKLHSPFPPAGDQPQAIEQLVRGIQAGKRAQCLLGVTGSGKT
                     FTMANVIAHFDKPTLVISHNKTLAAQLYGEFKEFFPKNAVHYFVSYYDYYQPEAYIPQ
                     RDIYIEKDASINEELDRLRLATTSSLVSRRDVIIVASVSCIYGLGSPQDYKSMMVPLR
                     VGETIDRDEMLMRLVDIQYDRNDIAFERGKFRVRGDCVEIWPSYEEYAFRLELWGDEV
                     EKLSIINPVSGETLTVQNEFFIYPAKHFVMPEDRIAQAIDTIKQELDERLTQLRQAGK
                     LLEAQRLSARTRFDIEMLMETGHCPGIENYSRPLSGRPPGSMPDTLHDFFPKDFLMIV
                     DESHVTTPQIRAMYNGDKARKTVLVEHGFRLPSALDNRPLKFEEWEQKIGQVVFVSAT
                     PSDYELEKTAGEVVEQIIRPTGLLDPIIEVSPARGQVPHLLEQIRERAKVGERVLVTA
                     LTKRLAEDLSAYLVEQGVACKWLHSELDAFERVELLRDLRQGRFEALVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRAERSLIQTIGRAARHINGMAILYADKVTDSMQKA"
     gene            1809..2924
                     /locus_tag="VFB80_01415"
     CDS             1809..2924
                     /locus_tag="VFB80_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HZN32438.1"
                     /translation="MHYQHVCIEALGYSLPSEVLTSEEIERRLAPLYRRLKLPEGRLE
                     LMSGIRERRFWPVGTRPSDGSIASGRMALEAAGLDPAEIGCLIHGSVSRDQLEPATAC
                     RVHHELGLPRECQVYDVSNACLGMLSGMIQVANLIELGQIRAGLVVGSEDSRPLLEGT
                     IAALNGNQSLTRASVKPAFASLTIGSASAAVLLVHRDISRTGNRLTAAAARAHTAHHG
                     LCAGDHSAAEQMLMETDSEELLLQGLATGAATFADFLEATDWSPGEIDKSICHQVGST
                     HQRQMLEAIGLPLARDYATYPWLGNTGSVALPVTLALAVERGFIGEQEQVALLGIGSG
                     INSLMIGVDWQRSPVQSPAALPRPHFAELAAPHSPAP"
     gene            complement(2906..>3344)
                     /locus_tag="VFB80_01420"
     CDS             complement(2906..>3344)
                     /locus_tag="VFB80_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32439.1"
                     /translation="QFASLLGVGIGGWWADRWMRRSDRGRIYVSAIGMSFFLPALFGV
                     GNAGSLGMAIGFLILFGLGWGFFDCNNMPILCQVARPELRATGYGVMNLVSISCGGFA
                     DWGFGALRDRHVPLNVIFGVFAAVALLSIAIVLCIRPRYGAGE"
BASE COUNT          589 a         1068 c         1094 g          593 t
ORIGIN      
        1 gctttttgca tcgagtccgt taccttgtcg gcatagagaa tcgccatgcc gttgatgtgg
       61 cgcgccgcgc ggccgatcgt ttggatcagc gatcgttcgg cgcggagaaa accttccttg
      121 tcggcatcga ggatcgccac cagcgacacc tccggcagat cgagcccttc acggagcagg
      181 ttcacgccga ccagcgcctc gaaccgcccc tgccgcagat cgcgtagcaa ctctacccgc
      241 tcgaaggcgt ccagctcgct gtgcagccat ttgcaggcga cgccttgctc gaccagatac
      301 gccgacaggt cttccgccag ccgcttggtc agagcggtca ccagcacgcg ctcgcccact
      361 ttggcccgct cgcgaatctg ctcgagcaaa tgcggcacct gcccgcgggc cggcgagact
      421 tcgatgatcg gatcgagcaa gcctgtgggg cggatgatct gctcgaccac ctcgcccgcc
      481 gttttttcca gctcatagtc actcggcgtc gcgctgacga acacaacctg gccaatcttc
      541 tgttcccatt cttcgaactt gagcggccgg ttgtcgagag cgctcggcag gcgaaatccg
      601 tgctcgacca gcacggtctt ccgggccttg tcgccgttgt acatcgcccg aatctgcggc
      661 gtggtcacgt gcgactcatc gacgatcatc agaaaatctt tggggaagaa gtcgtgcagc
      721 gtatcgggca tcgagcccgg gggccggccc gacagcggcc ggctgtagtt ctcgatcccc
      781 gggcagtggc ccgtttccat cagcatttcg atgtcgaacc gcgtgcgggc actgagccgc
      841 tgggcctcca gcagcttgcc ggcctgccgg agctgggtca gccgctcgtc gagctcttgc
      901 ttgatcgtgt cgatggcctg ggcaatgcgg tcctcgggca ttacgaagtg cttggccggg
      961 taaatgaaga actcgttttg caccgtcagg gtctcgccgc tgacgggatt gatgatcgag
     1021 agcttctcga cctcgtcgcc ccacagctcc aggcgaaacg cgtattcctc gtagctcggc
     1081 cagatttcga cgcagtcgcc gcggacgcgg aacttgcccc gctcgaaggc gatgtcgttg
     1141 cggtcgtatt gaatgtcgac cagccgcatc agcatctcgt cgcggtcgat cgtctcgccg
     1201 acccgcagcg ggaccatcat cgacttgtaa tcctgcggcg agcccaagcc atagatgcac
     1261 gacacgctgg ccacgatgat cacgtcccgc cggctgacca gcgagctggt cgtcgccagc
     1321 cgcagccggt cgagctcctc gttgatcgag gcgtccttct cgatatagat gtcgcgctgc
     1381 gggatgtagg cttccggctg ataatagtcg tagtagctga cgaagtagtg gacggcgttc
     1441 ttggggaaga attccttgaa ctcgccgtag agctgagccg cgagcgtctt gttgtgcgag
     1501 atgaccagcg tcggcttgtc gaagtgggcg atgacgttcg ccatcgtgaa cgtcttgccc
     1561 gacccggtca cacccaagag gcactgggcc cgcttccccg cctgaattcc acggacgagc
     1621 tgctcaattg cctgcggctg atcgccggcg ggaggaaacg ggctgtggag cttgaaggtc
     1681 actgggaagg gttcggggtt cagggttcag gaaggcggga cacctacact gtacgcaact
     1741 ggaaggttcc cagacgatgg gtcaacacgc cggtttacga gaaatgttgt gccccctatc
     1801 ttgaaggcat gcactaccag catgtctgca ttgaagcgct cggctattcg ctccccagcg
     1861 aggttttaac cagcgaagag atcgaacgcc gcttggcgcc actctatcgg cggctgaagc
     1921 tgcccgaggg gcggctggaa ttgatgagcg gtattcgcga gcggcggttc tggcctgtgg
     1981 gcacgcggcc gagcgatggc agcatcgcca gcggccgaat ggcgctcgaa gcggcggggc
     2041 tcgatcctgc ggagatcggc tgcctgattc acggctcggt cagccgcgat caattggagc
     2101 cggcgacggc ttgccgcgtg catcatgagc tgggcctgcc gcgcgagtgc caggtctatg
     2161 acgtgtcgaa cgcctgcctc ggcatgctca gcggcatgat ccaggtggcg aacctgatcg
     2221 agctgggcca gattcgtgcg gggctggtcg tcggctccga ggacagccgg ccgctcttgg
     2281 aaggcacgat tgcggcgctg aacggcaacc agtcgctgac gcgagcgagc gtcaagccgg
     2341 cgttcgcctc cctgaccatc ggctcggcca gcgcggcagt gctgctcgtc catcgggaca
     2401 tcagccgcac gggcaatcgc ctgacggcgg cagcggcccg ggcccacacg gcgcatcacg
     2461 ggctctgcgc cggcgatcac tcggccgccg agcagatgtt gatggaaacc gattccgagg
     2521 agctcttgct gcagggcctg gcaacgggtg cggccacgtt cgcggacttt ctggaggcga
     2581 cggactggtc gcctggcgag atcgacaaat cgatctgcca ccaggtgggg agcacgcacc
     2641 agcgacagat gctggaggcg atcggcctgc cgctggcccg cgactatgcc acctatccct
     2701 ggctagggaa caccggctcg gtggcactgc cggtcacgct ggcgctggcg gtggagcgcg
     2761 gcttcatcgg cgagcaggag caggtcgcgc tcctgggcat cggcagcgga atcaactcgc
     2821 tgatgatcgg cgtcgattgg cagcgctcgc ccgtgcagtc gccagcggct ttgccgcgcc
     2881 cgcattttgc ggagttggcc gcgcctcatt cgcctgcgcc gtagcgcggc cggatgcaga
     2941 gcacgatggc gatggacagc agggcgaccg cggcaaagac gccaaaaatg acgttcagcg
     3001 gcacgtgccg gtcgcggagg gcgccaaagc cccagtcggc aaagccgccg cagctgatgc
     3061 tcaccaggtt catcacgccg tagccggtcg cccgcagctc gggccgcgcc acctggcaga
     3121 ggatcggcat gttgttgcag tcgaagaagc cccagcccag gccgaagagg atcagaaagc
     3181 cgatcgccat gcccagcgag ccggcgttgc ccacgccaaa cagcgccggc aggaagaacg
     3241 acatgccgat cgcgctgacg tagatgcggc cgcgatcgga acggcgcatc cagcgatcgg
     3301 cccaccagcc gccgatgccg acaccgagca gcgaagcgaa ctgg
//
LOCUS       DASRJB010000050         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2839500, whole genome shotgun sequence.
ACCESSION   DASRJB010000050 DASRJB010000000
VERSION     DASRJB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839500"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="VFB80_01425"
     CDS             <1..2415
                     /locus_tag="VFB80_01425"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HZN32440.1"
                     /translation="PPLVDVPPPDIDATAPPLRPTLFVGLGGCGGQVLRLLKRRLDDR
                     LPAELRMLAPILILDSDARELNATQGEEGSPLKPEEMLAMPLRRSQDYREDSRRILEW
                     LSRRWLYNIPRSQQTEGLRPLGRLALVDHAPAATARLKQAIGKLHEQTSALQLTPRVV
                     IVASPAGGTGGGMVTDIAFLIRQLLDDLPEGKGYEALAVLIHGTTRNPQQQELAAANT
                     VATLTELSQFHRPGAIFPGDHACGLKARGAGEGALSNVYLVHAGEELSPTQIEAASDR
                     VAEFLMLDAVTPAGTVLEAARCEEGDTLGLRLRSFGLHEFGFTHDRLLDDSVHRICRA
                     VISRLRGPQQMPEQKKAPRLLPSSTVTAVEDRPAQDPLLELDQRAAALARTMGLEVEP
                     LMQVVQQFSAADMGGDAEAFFRKLMVVGSPGQRPPVDKWVSTACDLFGRHHSDTSIEA
                     QPGELAQALDHRIGPWIAQVGTGLREWVEAIVEDPNCRVTGAKRAAKWFQSYLKSLVD
                     RIGEARGRLARDTQAVSQVLAATDPKGKGPRRTPAELTNAFMQYCRLRMFELCALRAG
                     QIAHSLQSHAVAAHDSLVDLHRELDHLASQFAVSDETGPQLGAAEVATMRSSVADQLR
                     HSEEPLSRQIEELLIQGLFTKHGGLRAVVSTGGEVREQLISTIRTAARQAALGKVQSI
                     DLASLLLANQSGDSPLATCLTNAEPWLQRCGGRRRLLFVIPQQMAGQYSPAALAAQMG
                     TSAFRQMPGVAAGAASDLVVIFELGDVSVAHAAAHLIDFRRDLAEAAGRLQTRSDVTW
                     TPVFVF"
     gene            complement(2515..3249)
                     /locus_tag="VFB80_01430"
     CDS             complement(2515..3249)
                     /locus_tag="VFB80_01430"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN32441.1"
                     /translation="MPLNAKLVVVGGDVKTTEIKLRLPSTVGRGRGTTIMLPHPLVSR
                     QHCELFENNGQLMVRDLGSLNGTFVNNQRITESPLPPGELLTIGTVTFRAMYEAQPDS
                     QPPTGAGPQMKPRRSETDVAGLPPGAIPAAPQDGPIAFEEAVVPVEESAAPAAGPLHV
                     TKRMETTLEDRSGVPKTESVASAKTKPAPAKAATPAPAKDTAKPAAKGDVAVDFSKLA
                     DDPAETEPANDEDDGDLGDFLKSLKK"
     gene            complement(3494..3634)
                     /locus_tag="VFB80_01435"
     CDS             complement(3494..3634)
                     /locus_tag="VFB80_01435"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZN32442.1"
                     /translation="MGLTTGYVPKFVRQYADLKSIIQDAVSRYTDDVRSGKFPAAEHG
                     FQ"
BASE COUNT          581 a         1231 c         1257 g          575 t
ORIGIN      
        1 ccgccgctgg tcgatgtgcc gcccccggac atcgacgcca ccgccccgcc gctgcggccc
       61 acgctgttcg tcggcctggg cggctgcggc ggccaggtgc tccgcctgct caagcggcgg
      121 ctggacgacc gcctgccggc cgaactgcgg atgctcgcgc cgattttaat cctggactcc
      181 gacgcccgcg agctgaatgc gacgcagggc gaggaggggt cgcccctcaa gcccgaggaa
      241 atgctggcga tgccgctgcg gcggtcgcag gactatcgcg aggactcgcg gcggattctg
      301 gagtggctga gccggcgctg gctgtacaac attccccgct cgcagcagac cgaggggctg
      361 cggccgctgg gccgcctggc gctggtcgat cacgctccgg ccgcgacggc ccgcctcaag
      421 caggcgatcg gcaagctgca cgagcagacg tccgccctgc agctgacgcc gcgggtggtg
      481 atcgtcgctt cgccggcagg cggcacgggc ggcggcatgg tcaccgacat cgctttcctc
      541 attcggcagc tgctcgacga cctgcccgag ggcaagggct atgaagcgct cgcggtgctc
      601 atacacggga ccacccgcaa tccgcagcag caggagctgg cggcggcgaa caccgtcgcc
      661 acgctgaccg agctgtccca gttccaccgg ccgggcgcga tcttcccggg cgaccatgcc
      721 tgcggcctga aggcccgcgg agccggtgaa ggggcgctga gcaacgttta cctggtgcac
      781 gccggcgagg agctgtcgcc gacccagata gaagccgcca gcgaccgcgt cgccgagttc
      841 ctgatgctcg acgccgtcac gccggccggc acggtgctcg aggcggctcg ctgcgaggag
      901 ggtgacacgc tgggcctgcg gctgcggagc ttcggcctgc acgaattcgg cttcacgcac
      961 gaccggctgc tggacgattc ggtccaccgc atttgccggg ccgtgatctc acggctgcgc
     1021 ggaccgcagc agatgccgga gcaaaagaaa gcgccgcgcc tgctgccgag ctcgacggtc
     1081 accgcggtcg aggaccggcc ggctcaggac ccgctgctgg agctcgacca gcgggcggcg
     1141 gccctggccc gcacgatggg cctggaagtc gagccgctga tgcaggtggt gcagcagttc
     1201 tcggcggccg atatgggcgg cgacgccgag gccttcttcc gcaagctgat ggtcgtgggc
     1261 tcgccgggac agcgtccgcc ggtcgacaag tgggtcagca ccgcctgcga cctattcggc
     1321 cgccaccatt cggacacttc gattgaagcc cagccgggcg agctcgctca agcgctcgat
     1381 catcgtatcg ggccgtggat cgcgcaggtc ggtacgggct tgcgggagtg ggtcgaagcg
     1441 atcgtcgaag atccgaactg ccgcgtgacc ggcgccaagc gggccgcgaa gtggtttcaa
     1501 agttacctca agtcgctggt ggaccggatc ggcgaggccc gtggccggct ggctcgcgat
     1561 acgcaggctg tctcgcaagt gctcgcggcg accgatccga agggcaaggg tccgcggcgc
     1621 acgcccgcgg agctgaccaa cgcattcatg cagtattgcc gcttgcggat gttcgagctg
     1681 tgcgcgctgc gggctgggca aatcgcccac tcgctgcaga gccacgccgt cgccgcacac
     1741 gactcgctgg tcgatctgca ccgcgagctg gaccacctgg cgtcgcaatt cgccgtgtcc
     1801 gatgagaccg gcccgcagct gggggcagca gaagtcgcca cgatgcgctc gtcggtggcc
     1861 gatcagctgc ggcattcgga agagccgctc tcgcgccaaa tcgaggaact gctcatccag
     1921 gggctgttca cgaagcacgg cggcctgcgg gcggtcgtca gcaccggcgg cgaagtccgc
     1981 gagcagctga tctcgacgat ccgcactgcc gcccgccagg cggcgctggg caaggtgcag
     2041 tcgatcgacc tggcttcgct gctcctggcc aaccagagcg gcgacagccc gctggccacg
     2101 tgcctgacca acgccgagcc ctggctgcag cgctgcggcg gccgccgccg gctgctgttc
     2161 gtgattccac agcaaatggc cgggcagtac agcccagccg cgctcgcggc ccagatggga
     2221 acctccgcgt tccgccagat gcccggcgtc gccgccggcg cggccagcga cctggtcgtg
     2281 atcttcgagc tgggcgacgt gagcgtggcc cacgccgcgg cccacctgat cgacttccgc
     2341 cgcgacctgg ccgaagccgc cggccgcctg caaacgcgga gcgacgtcac ctggacgccg
     2401 gtgtttgtgt tctagagtcc taatcctaat cctaatcccg ctcgcgctct cgctctcgcc
     2461 gtcggggcga acccgaacgg gattaagatt aagattagga ttaacggcga gcggctactt
     2521 cttcaagctc ttcaagaaat cgccgagatc cccgtcgtcc tcgtcattgg cgggctcagt
     2581 ttcggccggg tcgtcggcca gcttggagaa atcgacggcg acgtctccct tggccgcggg
     2641 cttcgcagtg tccttggcag gcgcaggagt cgcggccttg gccggggctg gcttggtctt
     2701 ggccgaggcc acggattcgg tcttgggcac gccggaccgg tcctcgagcg tcgtttccat
     2761 ccgcttcgtg acatgaaggg ggcccgccgc cggagcggcg ctctcttcca cgggaacgac
     2821 cgcctcttcg aaggcaatcg ggccatcctg cggcgctgcc gggatcgccc ccggaggcag
     2881 gccggcgacg tccgtctcgc tccgccgcgg cttcatttgc ggccccgcgc cggtcggcgg
     2941 ctgcgagtcg ggctgagcct cgtacatcgc ccggaacgtg acggtgccga tcgtcagcaa
     3001 ttcgcccggc ggcagcggcg actcggtgat ccgctggtta ttcacgaacg tgccgttgag
     3061 cgagccgagg tcgcggacca tcagctgacc gttgttctca aacaactcgc aatgctgccg
     3121 gctgaccagc ggatgcggga gcatgatggt cgttccccgc ccgcggccga ccgtcgaggg
     3181 cagccgcaac ttgatctccg tggttttcac gtcgccgcca acgacgacga gcttggcatt
     3241 gagaggcatt tcaagatccg gccagcgaag ccatggggag gggacttcga cctgtggcaa
     3301 ggtgcggaga atgcagcaaa ggatggtcgc aactgccgtg ccgcaagcgt ctagctctgc
     3361 tcgcggcgcg gaacaaacaa cccaggaaga tggataatag gaatagtcta actctggcca
     3421 tccagaagaa caacggtcga gcagccgccc ccagaagccg ccaaggtcag agcagccgcc
     3481 cgtgccactg ccgctactga aaaccatgct cggcagcggg gaatttgccg ctgcggacgt
     3541 cgtcggtgta acggctgacg gcgtcctgaa tgatgctctt gaggtcggcg tattggcgga
     3601 cgaacttggg gacgtagccg gtcgtgaggc ccacgaggtc gctg
//
LOCUS       DASRJB010000051         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1428814, whole genome shotgun sequence.
ACCESSION   DASRJB010000051 DASRJB010000000
VERSION     DASRJB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428814"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFB80_01440"
     CDS             <1..198
                     /locus_tag="VFB80_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN32443.1"
                     /translation="PFVARLPSRTEFVFRQRSEGRTLAEIALDMGITKEAVRQHELKA
                     IKLLRAAVAGLLPSQASQKSA"
     gene            complement(270..614)
                     /locus_tag="VFB80_01445"
     CDS             complement(270..614)
                     /locus_tag="VFB80_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32444.1"
                     /translation="MQEHAGYHCANCGRLTMHVRDVKRCNHLVHALVTLFLCGLWLPV
                     WIIAACSEQLGPWLCSQCGFSGGVSPFQQPQQQHLANAQAPIVAQEPPIAAPAKLTPT
                     RSGKLPPPLPRS"
     gene            941..1165
                     /locus_tag="VFB80_01450"
     CDS             941..1165
                     /locus_tag="VFB80_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN32445.1"
                     /translation="MIAQEELRPVIGGNVERLRKAKGMSQEELAEAVGISRVFLNRIE
                     NGKSSPGAEVLYGLADVLKVTADNLRQLPS"
     gene            1565..2650
                     /locus_tag="VFB80_01455"
     CDS             1565..2650
                     /locus_tag="VFB80_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32446.1"
                     /translation="MQRFLSQQKPQLRLLKPDDLDSLVVEAGGLRLSEFYFSWFEPNA
                     IEGESKSAATRRGYRQTVKLWIELTGDPIGFDQFTISLFAAELHNYEYTRARSRDGRA
                     VPGAAYRKLSADRVNIHLRNLRAILNRMGPQRRPDTPTASVVAQPLAVKVPKLHPEPK
                     DCFELSRARAVVAAARHFDRPQVPGITPWEFWRGYCSWLYVTDVREGTLWGFEWLMLL
                     RREQGWWLKLPRALVSKTGKGKWLGLPDWCVASVEHWPRDRATIFPQPYHRDWLRELF
                     YELQRLAGVPTAEQLPPQAWRRMHAREMQRLGADFAIEVSRRSLDHGDRSTTTSHYAD
                     ITEELRRRLPPLWDLASENPRQRRLFD"
BASE COUNT          452 a          916 c          919 g          423 t
ORIGIN      
        1 cccttcgtgg cccggctccc ctcccgcacc gagttcgtgt tccggcagcg gagcgagggc
       61 cgcacgctgg ccgagatcgc cctcgatatg gggatcacca aggaggccgt ccggcagcac
      121 gagctgaagg ccatcaagct cctgcgggcc gccgtcgccg gcctcctgcc ctcccaggcc
      181 tcccagaaaa gcgcctagcg gccccgccgc ccccgcgggc cttccacctc cccggccctc
      241 cgaggccgcc agcgggcctc ctgcgctttt caggagcggg gcagcggcgg gggcagcttg
      301 ccgctccggg tcggcgtcag cttcgcgggc gccgcgatcg gcggctcctg ggccacgatc
      361 ggggcctggg cgttggccag gtgctgctgc tggggctgtt gaaagggcga cacgccgccg
      421 ctgaagccgc actgcgagca gagccacggc ccgagctgct ccgagcaggc cgcgatgatc
      481 cagaccggca gccacagccc gcacaagaac agggtcacca gggcgtgcac caggtggttg
      541 caccgcttca cgtcgcggac gtgcatcgtc agccgcccgc agttggcgca gtggtagccg
      601 gcgtgttcct gcatgccaat gacccccagg cgagcagtgt aaatcgccgc gcggcgaaaa
      661 gcacctgcgg cttcccgcaa tccgcagacc ttgcccgacc ggacgagtcg gcgctgttgg
      721 gcctccgaag ccgaaggtta caggttcgag ccctgtcggg gatattcgcc gccgaaaccc
      781 aggcggtgca aggagttgat ggcaactgac gacccagctt gccgcgtcgc acttctgggg
      841 ctcagaaagc cgctgatttg cgacggggca ggcccggcaa gtgcggcaac tcccgtttca
      901 cgagagtcaa acaaaggtta ctatccagga gatggaagcc gtgatcgcac aggaagagct
      961 tcgccccgtg atcggtggca acgtcgagcg actccgtaag gcgaagggga tgagccagga
     1021 ggagctggcc gaggcggtcg ggatttcccg cgtctttctc aaccgcatcg agaacgggaa
     1081 gtcgtcgcct ggcgctgaag tgctctacgg gttggccgat gtactcaagg tgaccgccga
     1141 caatttgcgg caactgccct cttgacggca tgtagtacat attttacatt cctcggccgc
     1201 tgaaatgatc agcggcattc ttcgggcctg ctgcccgccg agtgccagga cagcctgggt
     1261 cgcaccagga gcatcgtgcg ttgcgacggc gtggagggag aggccacgac gacttcgcac
     1321 ggtctccctg gtgcggcccc ggctcatgga aggcacggcc ggaggccacc tctccccatg
     1381 caggcttcgc tgactgttac cgccgacaca tctcccctcg cggctttccg ccttctagcg
     1441 gatgtcgcgc tctcccgagc cggcgagaat ttgctgcgcg cacaattgcc atggctcatg
     1501 gaggagtttg gtcgcacggg ggcgaacgga cgacaggcga gccaccggcg acggagtcaa
     1561 cggtgtgcaa cgtttcttgt cccagcaaaa accacagctc cgactcctca agccggacga
     1621 cctcgacagc ctcgtcgtcg aggcgggcgg gctgcggctg tcggagttct acttcagttg
     1681 gttcgagccc aacgccatcg aaggcgagag caaaagtgcg gcgacgcggc ggggataccg
     1741 ccagaccgtc aagctgtgga tcgaactgac gggcgacccg atcggcttcg accagtttac
     1801 gatcagcttg tttgcggcgg agctccacaa ttacgagtac acgcgggcca ggtccaggga
     1861 cgggcgggcc gtcccgggag ccgcctatcg caagctctcg gccgaccggg tcaacatcca
     1921 cctgcgaaac ctccgcgcga tcctgaatcg aatgggcccg cagcgccggc cggacacgcc
     1981 cacggcttcg gtcgtggcgc agccgctggc ggtgaaggtg cccaagctgc atcccgagcc
     2041 gaaggactgc ttcgagctct cgcgggcgcg cgcggtcgtc gccgcggccc ggcacttcga
     2101 tcggccgcag gtgcccggaa tcacaccctg ggaattctgg cggggctact gcagctggct
     2161 ctacgtgacc gacgtgcgcg aagggacgct gtggggcttc gagtggctga tgctcctgcg
     2221 ccgggaacag ggctggtggc tcaagctgcc gcgagcgctc gtctcgaaga cgggcaaggg
     2281 caagtggctg ggtttacccg actggtgcgt cgcgtcagtc gagcactggc cgcgcgaccg
     2341 agcgacgatc ttcccgcagc cctatcaccg cgactggctg cgggagctgt tctacgagct
     2401 gcaacgcctg gcgggagtgc ccaccgcgga gcagctgccg ccccaggcct ggcggcggat
     2461 gcacgcccgc gagatgcagc gcctcggggc ggacttcgcg atcgaggttt cccggcgctc
     2521 gctggaccac ggcgaccgga gcacgacgac cagccactac gccgacatca ccgaggagct
     2581 gcggcgccgg ctgccgccgc tgtgggactt ggcgagcgag aatcctcggc agcggcggct
     2641 gttcgattga cacacgccca gccggcactc gcagcgacgt cgctgcgtcg tgctggcgtc
     2701 tccccggcca
//
LOCUS       DASRJB010000052         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_300617, whole genome shotgun sequence.
ACCESSION   DASRJB010000052 DASRJB010000000
VERSION     DASRJB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300617"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFB80_01460"
     CDS             <1..551
                     /locus_tag="VFB80_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32447.1"
                     /translation="TYDREVIKLDQQRTAALHKKLYLPPPKEVVVVPTSRESAQTWRY
                     TTEKPAQGWEMAAFDDVSWKEGPGGFGEPTTPGSKVRTSWKTPDIWLRSSFELADAKP
                     EGLALLIHHDEDAEIYLNGTLIATVKGFVTDYSLIPLDEKAAAALKPGKNTFAVHCHQ
                     TGGGQYIDIGLSRLIETNSASP"
     gene            592..807
                     /locus_tag="VFB80_01465"
     CDS             592..807
                     /locus_tag="VFB80_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN32448.1"
                     /translation="MRRPSCSGVCALLLLSLAGSAWGQARQPREGKLSVGDAAPDFSV
                     KDVHGKESVRLADLKGQPVVLIFGSCT"
     gene            820..1218
                     /locus_tag="VFB80_01470"
                     /pseudo
     CDS             820..1218
                     /locus_tag="VFB80_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
     gene            complement(1231..>2941)
                     /locus_tag="VFB80_01475"
     CDS             complement(1231..>2941)
                     /locus_tag="VFB80_01475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32449.1"
                     /translation="VHRDLKPGNILLDDAGQPHITDFGLAKRAQDVEITAQGEILGTP
                     AYISPEQARGEAHLADARTDVYAVGVMLFELLTGDLPFRGNVLMLTHHAVHTEPPSPR
                     SLNRSISRDLETICLKCLEKDPSRRYASAADLAAELRRYLAGEELVARPITRVERLWR
                     WGRRNPRIPTLSAALLAVVLVAYALAWWQLAAILKRTEAAVSSGALANLKFTAESVAT
                     TAGQEIEQMFALAEQAAADLKTPLKELENDPQTQALSKSLSDPNWPVENNPARDAARW
                     QLENHPERQAVQRWAEQFNKDLASKGLKVFATFVQLSDGLQIARDPLSDGGEKTFGIN
                     YSWRPYFNGEAAIRKKSWRAEPGQHIQATYLSPPFLTEHTNEWVVVVSAPVVVVGDDG
                     ADQFCGVVGIMIKLGSFAKLPGQSPAASPAAAADRSFAVLIDSREANRGQVLQHPLIN
                     TPSDPAAAKADEGRRWKMIHDAQQYRISQNSWKSPDYFVDPLGKLDARYDQRYLAADE
                     PVSFGGRDAGVSVVVQESYEQAIGQPLAELRRALVLLSLITFGLSAAVIVPLWAVILR
                     LVR"
BASE COUNT          514 a          968 c          958 g          501 t
ORIGIN      
        1 tgacgtacga ccgcgaagtg ataaagctcg accagcagcg gacggccgcg ctgcacaaga
       61 agctctacct gccgccgccg aaggaagtcg tcgtcgtgcc cacgtcgcgc gagtcggcac
      121 agacctggcg ctacacgacg gagaagccgg cccaaggctg ggagatggcg gcattcgacg
      181 atgtttcctg gaaggaaggc ccgggcggct tcggcgagcc gacgacgccc ggcagcaaag
      241 tccgcaccag ctggaagacg cccgacatct ggctccgcag cagcttcgag ctggccgatg
      301 caaagccgga gggcctcgcg cttctcatcc accacgacga agacgccgag atctatctca
      361 acggcacgct gatcgcgacc gtgaagggtt ttgtcaccga ctattcgcta atcccgctcg
      421 acgaaaaggc ggccgccgcc ctgaagcccg gcaagaacac gttcgccgtc cactgccacc
      481 agacgggcgg tggacaatat atcgacatcg gcctgtcgcg gctcatcgag acaaactccg
      541 cgtcgccgta gatataatcc caataggcga gtccgtttcc ggagggcgtt tatgcgtagg
      601 cccagttgct cgggggtgtg cgcgctgttg ctgctctcgc tggccgggtc ggcctggggc
      661 caggcgcggc aaccgcgcga ggggaagctg agcgtcggcg acgcggcccc ggatttctcc
      721 gtcaaggacg tccacgggaa agagagcgtc cggctggcgg atctcaaggg ccagccggtg
      781 gtgctgatct tcggcagctg tacgtgaccg ccgtttctca gctcggtcgg ttcgatcgag
      841 aaactgtatc aaacgtacaa aggccaggcc cacatctacg tgctctatat ccgcgaggcg
      901 catccgctcg gcggcccgcg gcgcactccc aaggagtttc cggtcgccga tccgaaaact
      961 ctcgccgagc ggcagaaggt ggcggaggat ttcgccagca aagttcggct ctccgtgccg
     1021 attctggtcg atacgatcga cgaccaggtg gagcaggcct acgccggctg gcccgaccgg
     1081 atctacattc tcgatcgtga cggcaagatc gctcacaagg gccgccccgg ccccggcggt
     1141 ttcgccccgg cggtgaagga tacgcccgcg atcctggaca agctgctgaa ggatgcgccc
     1201 gcaacgccgc cgcggtagtg cgcgagggcc tcaccgcacc agccgcagga tgaccgccca
     1261 tagcgggacg atgaccgcgg cggacaaacc gaacgtgatc aggctgagca gtaccagggc
     1321 gcggcgcagt tcggcgagcg gctggccgat cgcctgctcg tagctttcct gcacgaccac
     1381 cgagacgccg gcgtcgcgac cgccgaaact aaccggctcg tccgcggcga gatatcgctg
     1441 atcgtaccgg gcgtcgagtt ttcccagcgg atcgacaaag taatcgggac tcttccagga
     1501 attttgcgaa atgcggtatt gctgggcgtc gtggatcatc ttccagcgcc tgccttcgtc
     1561 cgcttttgca gccgccggat cgctgggcgt gttgatcagc ggatgctgga gcacctgccc
     1621 gcggttcgcc tcgcgtgaat cgatcagcac ggcaaagctg cggtcggccg ctgccgccgg
     1681 actggcggct ggcgactggc ccggcaattt ggcaaacgag ccgagcttga tcatgatccc
     1741 caccacaccg caaaactgat ccgctccgtc gtcgccaaca acgacgacgg gcgcggacac
     1801 gacgacgacc cactcgttcg tgtgttcggt caagaaggga ggcgagaggt acgtcgcttg
     1861 gatgtgctgc cctggctcgg cccgccatga ctttttgcga attgccgctt cgccgttgaa
     1921 gtaggggcgc cacgaatagt tgatgccgaa cgtcttctcg ccgccgtcgc tgagcggatc
     1981 gcgggcgatt tgcagcccgt cggacaactg cacgaacgtc gcaaagacct tgagcccctt
     2041 gctcgccaga tccttgttga attgttcagc ccaccgctgc acggcttgcc gctccggatg
     2101 gttctccaac tgccagcgcg cggcgtcgcg cgccgggttg ttttcgaccg gccaattcgg
     2161 atcgctcagc gacttcgaca gggcctgggt ctgtggatcg ttctcgagtt ctttcagcgg
     2221 cgttttcaga tcggcagcag cttgttcggc gagcgcgaac atctgctcga tttcctggcc
     2281 ggccgtagtg gccaccgatt cggccgtgaa cttgaggttc gccagcgcac cggagctaac
     2341 cgccgcctcc gtccgcttca ggatcgccgc cagctgccac caggccagcg cgtacgccac
     2401 gagcacgacg gccaagagag ccgccgagag cgtcggaatc cgcgggttgc gtcggcccca
     2461 gcgccacagc cgctcgactc gcgtgatcgg ccgcgcgacg agctcctcgc ccgccaggta
     2521 gcgccgcaac tccgccgcca ggtcggccgc cgaggcatac cggcggctgg ggtccttctc
     2581 caggcacttc aggcaaatcg tctccaggtc gcgggaaatg ctgcggttaa ggctccgcgg
     2641 gctgggcggc tcggtgtgca ccgcgtggtg cgtgagcatc agcacgttgc cgcgaaacgg
     2701 caggtccccc gtcagcagct cgaacagcat cacacccacg gcatagacgt cggtgcgggc
     2761 gtcggccaga tgcgcttcgc cgcgggcctg ctcgggcgag atgtacgcgg gcgtgcccag
     2821 aatttccccc tgggccgtga tctcgacgtc ctgagcccgc ttggccaggc cgaagtcggt
     2881 gatgtgcggc tggccggcgt cgtcgagcag gatgttgccc ggcttgagat cgcgatggac
     2941 g
//
LOCUS       DASRJB010000053         8810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_865770, whole genome shotgun sequence.
ACCESSION   DASRJB010000053 DASRJB010000000
VERSION     DASRJB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865770"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFB80_01480"
     CDS             complement(<1..804)
                     /locus_tag="VFB80_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN32450.1"
                     /translation="MLVEWKTITLLWPEVILVAAASSIFVAGAFTRNRAWWALMAIVA
                     CLTAAGALAAQGPPWDAAKAVFTGPVTFDPMSLGLRWLALVVGLVFILIAARLAEPEL
                     AGEFFGCILLVAAGIMLAASANELVLLFLGLELISIPTYVLLFLGRKDRASGEATMKY
                     FFLSILSSALLLYGFAFLYGLGKTTLIAGTPAMPGIREALASPKLLAASPLAPLTGLA
                     LALIAAGLGFKLAIVPLQFYAPDVYQGTTNANAGLLAVAPKIAGVAGFIR"
     gene            complement(807..2420)
                     /locus_tag="VFB80_01485"
     CDS             complement(807..2420)
                     /locus_tag="VFB80_01485"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZN32451.1"
                     /translation="MTSQQLLLTIFLPLAGALAMWVVAPLGRSAARVTALVVTLLTLF
                     CAAQLAFSYPAGKDFAVTDVAWLAGGSATVDIRFSVGLDGLGVWLFGLSALLFVTSVL
                     VSWEAIADRAPLFYSMLLLLEFGCLGVFAARDIILFYVFFEFTLIPLFFLIGIWGSEE
                     RRYAAVKFFLFTLAGSVLTFLGLLAIVLWDYQHNGENTLRFSIASLTQGLAAHPLHPR
                     WQLAIFLALFAGFAIKVPLFPLHTWLPLAHVQAPTAGSVILAGILLKIGVYGFLRFNL
                     PMLPHATAACLPAMMWLAVAGIIYGALVALAQTDMKRLIAYSSVSHLGYCMLGLFALN
                     ASGVQGSVLQMVNHGLSTGALFAMVGMLYERYHTRQIADLSGLAARMPWLAFFMVLFT
                     FSSIGVPGLNGFAGEFLVLIGAFQRAWADAPPLWRMQLIAIAVLSVSGVVLGAWYMLW
                     LVQRVFFGPLKEPHAAGDEPASDLSLREILALAPLAVFVVWIGIRPDDFLSRMAPTLD
                     AARAPAVAALEKSQTDTAVPVAALGNERR"
     gene            complement(2428..4857)
                     /gene="nuoL"
                     /locus_tag="VFB80_01490"
     CDS             complement(2428..4857)
                     /gene="nuoL"
                     /locus_tag="VFB80_01490"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZN32452.1"
                     /translation="MTDTIRNCLILIPALPLAAAVLVALLGAKVLRGQSHWPVVLALL
                     GSFVCSLILLREVNQRQDSATPGGFEQVVTLWKWADIANAYYLKVNPPAVQPEEAGWR
                     NLRIEVALRADALTAIMLAMVTFVSALVAVYAAGYMAGDRGYWRFFAYIGLFVFSMTM
                     LVSVSNFVLLFVFWEAVGVCSYLLIGFWYQKPEAAAAGVKAFLVNRVGDFAFTIALFL
                     IWCTYGTLNYHDSLADGTTDPAAIAAANTPDAARQEPRPPAMIRGVLGQTRIAASGYV
                     TGALATAICLLLLLGACGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYMVAR
                     CTPLFAVAPAAQLVVATIGGFTALLAGLIALTQFDLKRVLAYSTISQLGYMFLALGAG
                     TFAGVTGGMYHLFTHAFFKALLFLGAGSVMHAMGGVIDMRQFSGLRRLMPWTYVTFFF
                     GCVALAGVPPFAGFWSKDAVLAAVHDKVHAIEHELDHRHQPPHGEAAAARPLHNWSEA
                     ELTRSRLVYQWLYYGAVATAFLTAFYTFRAFALTFHGPLQIPAQAGHHAHESPPVMVT
                     PLAVLAVCSILVGMYCIADRRNFGTNILVDFLSHTPSLAAGAISLTKVEPAFHVQVAA
                     VSTIAAIAGIGLALFFYLGERSEARFLSRLFDLQGADRWTDPQWVTQLERMPWIAAVT
                     RLFRQARLGWMVTLLGYALGTLSIVLSVPLVVGRFLSPYRLSRDKFYFDELYAALVVW
                     PLSVLAAVLAWVDRWIVDGLVNLAGRVPVAIGSLMRPMQMGLVQFYALAMVLGMLMLI
                     LVTARLLWASG"
     gene            complement(4867..5310)
                     /gene="nuoK"
                     /locus_tag="VFB80_01495"
     CDS             complement(4867..5310)
                     /gene="nuoK"
                     /locus_tag="VFB80_01495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZN32453.1"
                     /translation="MNELSLLYNYLAVGGVLFALGLIGFLVRRNMIVTFLCAEMMLQG
                     VSVSLVAFGRYHNDWGGQMLVIFIITVAACEAGIAMALILMLAQRAGSLDTAAWQDLR
                     EEGQPAFLDRQVPEERTEDRLWPRLTPAGLEPRRDPDEELHRPRV"
     gene            complement(5307..6107)
                     /locus_tag="VFB80_01500"
     CDS             complement(5307..6107)
                     /locus_tag="VFB80_01500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZN32454.1"
                     /translation="MTAALQRLWAQLPGPAQHALACPTFWGLLLGGIGVWMLLPARLR
                     YGRVLGSLLVAAGGALLACDLPLLGNWTSQGVFWLLSAITLGGAVATIVSRSPVYSAI
                     WFALSLLGTAGLFLFQGAQFLAGATIVVYAGAIVVTFLFVIMLAQPEGHSPYDRLTWG
                     GLPKILGVATAGLLVGILTFMLGPTKREISHPPQVASAAPSPQARAAAAGINSSQHVA
                     NLGRHLFGEHLVSVEIAGTLLLIALVGAVAIAIQGKPRLAQPIEEALR"
     gene            complement(6104..6652)
                     /locus_tag="VFB80_01505"
     CDS             complement(6104..6652)
                     /locus_tag="VFB80_01505"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZN32455.1"
                     /translation="MKADDPHIKWIPEPQLGLTGKMYLPLLAQGLTTTLKHISRSLRG
                     NVVTVSYPDEEPQIANPQIYRGVHRLNKDAAGRVKCVACFLCATACPAHCIDIIAEES
                     PWPDREKYPQSFTIDELRCIYCGMCEEACPVDAIELTSLYNLTGRSRDEMVFDKEKLL
                     SVYDLTKDKEPMQSPALGGTAT"
     gene            complement(6649..7863)
                     /gene="nuoH"
                     /locus_tag="VFB80_01510"
     CDS             complement(6649..7863)
                     /gene="nuoH"
                     /locus_tag="VFB80_01510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZN32456.1"
                     /translation="MLEPLIKIGLLVGGLMTAAAYFVLLERRMAAWTQDRIGPNRVGI
                     PLTNIRLWGLGQPLADGLKFILKEEYTPGHVDRRLYILAPLTILAAALATYAVIPFGS
                     LIPPIHGGQPIPLVVAPGVDVGMIYVFALSSVAVYGVILGGWASNSKYSFLGGLRSSA
                     QLIAYELPMGLGILGVVLAAGSLNLETIIVEQAKQGWFAFAQPLGFVVFVVAAFAEAA
                     RLPFDLPECEQELIGGYHTEYSGMKLIIYLIAEFLHMITASFLIVILFLGGWHLPILT
                     GTPEAQSIGWVTAILRILVLQGKIFAVIVFFMWVRWSWPRFRYDQLMNLAWKVMLPLG
                     LVNLVALAVLYELRHQNDPYGQSIAWTAGIIAASWLVFVVSWTATALANPLVTDNRPR
                     RDIVPRLIDPQI"
     gene            complement(7871..>8810)
                     /locus_tag="VFB80_01515"
     CDS             complement(7871..>8810)
                     /locus_tag="VFB80_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32457.1"
                     /translation="VCAGCSTGCSIKIEENQDQVYRLKPRENPHVNQWWMCDEGRYGW
                     KHVHDADRLTQLRWRVKDAYENVEWSEFAAEIDQKLRKAGRLAAVISPQLTVEEAYLL
                     AKYVRAIDPAAVLAVGFIPVDGAEERFPNGFTIRAEKCPNRKGVEAICSRLGGGLLNW
                     GDFLSQADSFGAVWVTGGYQASWHDAPVVDRLAGVKTLIVQDCLPSPLWDAATYQVPG
                     ATFAEREGSYVNAQDRLQSFRWAIRPPAGVRTEGQLYWQLLKQPGLYRASEVLAEIAR
                     EIGYFSAAARGVPDVGVDLKVTQIADAAPLAPSPTA"
BASE COUNT         1726 a         2850 c         2969 g         1265 t
ORIGIN      
        1 gcggatgaat ccggcgacgc cggcgatctt cggcgcgacg gccaggagac ccgcgttggc
       61 gttggtcgtg ccctgataaa cgtccggcgc gtagaactgc agcgggacaa tcgccagctt
      121 gaatcccaag cctgcggcga tgagagccag cgccagcccg gtcaacggcg cgagcgggct
      181 ggcggcgagc agcttcgggc tggccagcgc ctcgcgaatg cccggcatcg cgggtgtgcc
      241 ggcgatcagc gtcgtcttcc ccaggccata cagaaacgcg aatccgtaca gcagcagcgc
      301 cgacgagagg atgctgagga agaagtactt catcgtggcc tcgcccgagg cccggtcttt
      361 gcggcccagg aacaagagca cgtaggtcgg gatggagatc agctccagac ccaggaacag
      421 caggaccaac tcgttggcgg atgccgcgag catgatgccg gccgcgacca gcaggatgca
      481 gccgaagaat tccccggcca gctccggctc ggcgaggcgg gcggcgatca ggatgaagac
      541 caggcccaca accagcgcca gccagcggag gcccaggctc atcgggtcga acgtcacggg
      601 cccggtgaac acggccttgg cggcgtccca cggcggacct tgagccgcca gcgcaccggc
      661 cgcggtgaga caggccacga tcgccatcag cgcccaccag gcccggttgc gcgtgaacgc
      721 cccggcaacg aagatcgacg acgctgcggc cacgagaatg acctcgggcc agagcagggt
      781 gatcgttttc cactcgacga gcacagctat ctcctctcgt tccccagggc ggccacggga
      841 acggccgtat cagtctgcga tttctccaac gcggccacgg ccggtgcccg agcggcgtcc
      901 agcgtcggcg ccatccggct gaggaagtcg tccgggcgga tgccgatcca gacgacgaac
      961 acggccagcg gcgcgagggc gagaatttct cgcagggaaa ggtcgctggc cggctcgtcg
     1021 ccggcggcgt gcggctcttt gagcgggccg aaaaagaccc gctgcacaag ccacagcatg
     1081 taccaggctc cgaggaccac acccgagacg gacaggacgg cgatcgcaat caattgcatc
     1141 cgccacagcg gcggcgcatc ggcccaggcc cgctggaacg cgccgatgag tacgaggaac
     1201 tcgccggcga aaccgttgag gcccggcacg ccgatgctcg agaacgtgaa cagcaccatg
     1261 aagaacgcca gccacggcat gcgggcggcc aggccggaca gatcggcgat ctgccgcgtg
     1321 tggtagcgct cgtagagcat gccgaccatc gcgaacagag cgcccgtgga cagaccatga
     1381 ttcaccattt gcagcacgct gccttgcacg ccggaggcgt tgagcgcgaa cagccccagc
     1441 atgcaatatc cgaggtggct gacgctggaa taggcgatca gccgcttcat gtctgtctgg
     1501 gccagggcga cgagcgctcc gtagatgatg ccggccacgg cgagccacat catcgccggc
     1561 aggcaagcag cggtcgcgtg gggcagcatt ggcaggttga accgcaggaa gccgtacacg
     1621 ccgatcttca gcaggatgcc ggccaggatc acgctgcccg ccgtcggcgc ttgcacgtgg
     1681 gccaagggca gccaggtgtg cagcggaaac agcggcacct tgatggcaaa gcccgcaaac
     1741 aacgccagga agatcgccag ctgccaccgc ggatgcagcg gatgcgcggc gagaccttgc
     1801 gtgagcgagg cgatcgagaa ccgcagcgtg ttctcgccgt tgtgctggta gtcccagagc
     1861 acgatcgcga gcaggccgag gaaggtcagc acgctgccgg cgagcgtgaa caggaagaac
     1921 ttgacagcgg cgtagcgccg ctcctcactg ccccagatgc cgatcaggaa gaagagcggg
     1981 atcagcgtga actcgaagaa gacgtagaac aggatgatgt cgcgggccgc gaatacgccg
     2041 aggcagccga actcgagcag gagcaacatc gaatagaaca gcggggcgcg gtcggcaatt
     2101 gcctcccagc tgaccagcac cgaggtgacg aacagcagcg cggaaaggcc gaacagccac
     2161 acgcccaaac cgtcgagtcc gacgctgaag cggatatcga cggtcgcgct gccgccggcc
     2221 agccaggcca catcggtcac ggcaaaatcc ttgcccgcgg gatagctgaa ggccagttgg
     2281 gcggcgcaga acagcgtgag cagggtcacg accagcgccg tcacgcgggc cgcggaccgg
     2341 cccagaggcg cgacgaccca catcgccagc gccccggcaa gcggcaggaa aatcgtcagt
     2401 aggagttgct ggctggtcat tgttccacta gccgctggcc cacagcagcc gggccgtgac
     2461 gaggatgagc atgagcatgc ccagcaccat cgccagggcg tagaactgca cgaggcccat
     2521 ttgcatcggc cgcatcagcg acccgatcgc gaccggcacg cggcccgcca gattcaccag
     2581 gccatcgacg atccagcgat cgacccaggc cagcacggcg gccagcaccg agagcggcca
     2641 gacgaccagc gccgcgtaca attcgtcgaa gtaaaacttg tcccgcgaca ggcggtaggg
     2701 cgaaaggaag cggccgacca ccagtggcac cgacagcacg atcgagagcg tgccgagagc
     2761 gtagccgagg agcgtcacca tccagcccag gcgggcctgg cggaagagcc tcgtcactgc
     2821 ggcgatccag ggcatgcgct ccagctgcgt cacccactgc gggtcggtcc accgatcggc
     2881 gccttgcagg tcgaacagcc gactgaggaa ccgggcttcg ctccgctcac ccagatagaa
     2941 gaacagcgcc aggccgatgc cggcgatcgc ggcaatcgtg ctgacggcgg cgacttgcac
     3001 gtgaaacgct ggctcgacct tcgtgagcga gatcgcgccg gcggcgagcg agggagtatg
     3061 gctcaggaaa tcgacgagga tatttgtgcc gaagttccgc cggtccgcga tacagtacat
     3121 gcccacgagg atcgagcaga ccgccaggac ggccagcggc gtgaccatca cgggaggcga
     3181 ctcgtgggcg tggtggccgg cctgggcggg aatttgcagc gggccgtgga aggtgagggc
     3241 gaaggcccgg aaggtgtaga acgccgtcag gaaggccgtt gccaccgcgc cgtagtaaag
     3301 ccattgatag acgagccggc tgcgcgtgag ctcggcctcg ctccagttgt gcagcggccg
     3361 ggctgcggcc gcttcaccgt gcggcggctg atggcgatgg tccaactcgt gctcgatggc
     3421 gtggaccttg tcgtggaccg cagccagcac cgcgtccttg ctccagaagc cggcaaatgg
     3481 cggcacgcca gcgagtgcca cgcagccgaa gaagaatgtt acgtaagtcc agggcatgag
     3541 cctccgcagg ccgctgaact gccgcatgtc gatgacgccg cccatggcgt gcatcacgct
     3601 gcccgcgccg aggaacagga gggccttgaa gaaggcgtgc gtgaagaggt ggtacatgcc
     3661 gccggtgact ccggcgaagg tgcccgcgcc gagggccaga aacatgtagc cgagctggct
     3721 gatcgtcgaa taggccagca cgcgcttcag gtcgaactgc gtcagggcga tcaggccggc
     3781 gagcagcgcc gtgaagccgc cgattgtcgc gaccacgagc tgcgccgcgg gtgcgacggc
     3841 gaagagcggc gtgcagcggg cgaccatata gacgccggcg gtgaccattg tcgcggcgtg
     3901 gatcagcgcg ctgaccggcg tcgggccctc catcgcgtcg ggaagccaga cgtgcagcgg
     3961 aaactgggcg ctcttgccgc aagctcccag gagcagcagc aggcagattg ccgtggccaa
     4021 ggccccggtg acgtagccgc tcgccgcgat gcgcgtttgg ccgagcacgc cacgaatcat
     4081 ggcgggaggg cgaggttcct gccgagccgc atccggcgta ttggccgcgg cgatcgcggc
     4141 cggatcggtt gtgccatctg ccagcgagtc gtgatagttc agcgtcccgt aggtacacca
     4201 aatcaggaac aaagcgatcg tgaaggcgaa gtcgccaacg cggttgacga ggaacgcctt
     4261 tacgcctgcc gcggccgctt cgggtttctg gtaccagaag ccgatcagca ggtaggagca
     4321 gacgccgacc gcctcccaga atacgaacag cagcacgaag ttgctcaccg agacgagcat
     4381 cgtcatcgag aatacgaaca ggccgatgta ggcgaagaat cgccaataac cgcggtcgcc
     4441 cgccatgtag cccgcggcat agacggcgac gagcgccgag acgaacgtga ccatcgccag
     4501 catgatcgcg gtcagggcgt cggcccgcag ggcgacttcg atccgcaagt tccgccagcc
     4561 ggcctcctcg ggctgcacgg cgggcggatt gaccttcaga tagtacgcat tggcgatgtc
     4621 ggcccatttc cagagcgtga cgacctgttc gaagccgccg ggtgtcgcgg aatcctgccg
     4681 ctggttgact tcgcgcagca ggatgaggct gcacacgaac gagcccagca gcgccagcac
     4741 gacgggccag tggctctggc cgcggagaac cttcgcgccc aggagcgcga ccagcaccgc
     4801 tgccgccagc ggcagcgcgg gaatgaggat caggcaattg cgaatcgtgt cggtcacagt
     4861 gtcatttcaa actcgcgggc ggtgcagttc ctcgtccgga tcgcggcgcg gctccagccc
     4921 agcgggagtc agccgcggcc agaggcggtc ctcggtccgt tcctcgggaa cttgccggtc
     4981 gaggaacgcg ggctggccct cctcgcgaag gtcctgccag gcggcggtgt cgaggctgcc
     5041 cgcccgctgg gcgagcatca ggatcagggc catcgcgatg cccgcctcgc aggcggcaac
     5101 cgtgatgatg aagatcacca gcatctgccc gccccagtcg ttgtgatagc ggccgaaggc
     5161 gaccaggctc accgacacgc cctgcagcat catctcggcg cagaggaagg tgacgatcat
     5221 gttccgccgc accagaaagc cgatcaggcc cagtgcgaac agcacgccgc ctaccgcgag
     5281 atagttgtag agaaggctca gctcgttcat cgcagcgcct cctcgatcgg ctgggccagc
     5341 cgcggcttgc cctggatcgc gatcgccacg gcgccgacga gggcgatcag cagcagcgtg
     5401 ccggcgatct cgacgctgac caggtgctcg ccgaacaggt ggcggccgag attcgcgacg
     5461 tgctggctgg aattgatgcc ggccgctgcc gcgcgggcct gcgggctggg cgcggccgag
     5521 gccacttgcg gcgggtgcga gatctcccgc ttcgttgggc cgagcatgaa cgtcaggatg
     5581 ccgacgagca gacccgcggt ggcgacgccc agaatcttcg gcagcccgcc ccaagtcagg
     5641 cggtcgtagg gcgagtggcc ttccggctgg gccagcatga tgacgaacag aaacgtcacg
     5701 acgatcgcgc cggcatagac gacgatcgtc gcgccggcga ggaactgagc gccctggaac
     5761 aggaacagcc ccgccgtgcc gagcaacgag agggcaaacc agattgccga gtagacgggg
     5821 ctgcgcgaga cgatcgtggc gaccgccccg cccagcgtga tcgccgaaag cagccaaaac
     5881 acgccctggc tggtccagtt gcccaggagc ggcaagtcgc aggccagcag tgcgccgccc
     5941 gcggcgacga gcaggctgcc cagcacgcgg ccatagcgca ggcgagccgg caagagcatc
     6001 cagacgccga ttccccccag caacaggccc caaaacgtgg ggcaggccag tgcgtgctgc
     6061 gcggggcctg ggagctgcgc ccagagtcgc tgcagagcgg ccgtcatgtg gcggtccctc
     6121 ccagtgccgg cgactgcatc ggctccttgt ccttcgtgag gtcgtacacg gagagcagct
     6181 tttccttatc gaagaccatt tcgtcgcggc tgcggccggt gagattgtag aggctggtca
     6241 gctcgatggc atcgacgggg caggcctctt cacacatgcc gcagtagatg cagcgcagct
     6301 cgtcgatcgt gaagctctgt ggatatttct cgcggtcggg ccaggggctt tcttcggcga
     6361 tgatgtcgat gcagtgggct gggcaggcag tcgcgcacag aaaacaggcc acgcacttca
     6421 cgcggcccgc ggcgtccttg ttcagccgat gcacgccgcg gtagatctgc ggattggcga
     6481 tctgcggctc ttcgtccgga taggacaccg tcaccacatt gccgcgcagc gagcggctga
     6541 tgtgtttgag cgtggtcgtc aggccctggg caagcagcgg cagatacatc ttgccggtca
     6601 ggcccagctg gggctcgggt atccacttga tgtgcgggtc gtcggctttc atatctgcgg
     6661 atcgatcagc cgaggcacaa tgtcccgcct cgggcggttg tcggtcacca gcgggttggc
     6721 cagcgcggtc gccgtccacg agaccacgaa cacaagccag gacgccgcga tgatgccggc
     6781 cgtccaagcg atgctctggc cgtacggatc gttttggtgg cgaagctcgt acagcaccgc
     6841 caaagccacc aggttcacca gtcccagcgg gagcatgacc ttccaggcca ggttcatgag
     6901 ctgatcgtag cggaaccgcg gccagctcca gcggacccac atgaaaaaca cgattacggc
     6961 gaatatcttg ccctgcagca cgaggatccg caggatcgcg gtcacccagc cgatcgattg
     7021 tgcttccggc gtgccggtca aaatcggcag gtgccagccg ccgaggaaca ggatcacgat
     7081 caggaacgac gccgtgatca tgtgcaggaa ttcggcgatc agataaatga tcagcttcat
     7141 gcccgagtat tcggtgtgat agccgccgat cagctcttgc tcgcattcgg gcaggtcgaa
     7201 cggcaggcgg gccgcttcgg caaacgccgc gacgacgaac acgacgaacc ccagcggctg
     7261 cgcgaaggcg aaccaaccct gcttagcctg ctccacgatg atcgtctcga gattgagcga
     7321 gccagcggcc agcacgacgc ccaaaatgcc caggcccatc ggcagctcgt aggcgatcag
     7381 ctgcgcgctg gaacggaggc cgcccagaaa gctgtatttg ctgttgctgg cccacccgcc
     7441 gaggattacg ccgtacaccg ccacgctcga caaggcgaag acatagatca tgcctacgtc
     7501 cacgcccggc gcgacgacca gcggaatcgg ctggcctccg tgtatcggcg gaatcaggct
     7561 gccgaaggga atgaccgcgt aggtggccag cgccgcggcg agaatcgtca gcggggcgag
     7621 gatgtacagc cgccgatcga cgtgacccgg cgtgtactct tctttgagaa taaacttgag
     7681 tccgtcggcc agcggctgcc ccaagcccca gaggcggata ttggtgagcg gaatgccgac
     7741 gcgattgggg ccgatgcggt cctgcgtcca ggccgccatc cgccgctcga gcagcacgaa
     7801 ataggccgcc gcggtcatca gaccaccgac gagcaggccg attttgatga ggggttcgag
     7861 catgagaggg ctaggcggta gggctggggg ctagaggtgc agcatccgcg atctgagtaa
     7921 ccttcagatc cactcctaca tctggtaccc cgcgggctgc ggccgagaag tagccgatct
     7981 cgcgggcgat ctccgccagc acttcgctgg cgcggtacag ccccggctgc ttgagcagct
     8041 gccaatagag ttgtccttcg gtccgcacgc ccgccggcgg gcggatcgcc cagcggaacg
     8101 actggaggcg atcctgggca ttgacatacg agccttcgcg ttcggcgaac gtcgcgccgg
     8161 gaacctggta ggtcgcggca tcccacagcg gcgagggcag gcaatcctga acgatgagcg
     8221 tcttcacgcc ggccagccga tcgaccaccg gcgcgtcgtg ccacgacgcc tgatagccgc
     8281 ccgtcaccca caccgccccg aacgaatcgg cctggctgag aaaatcgccc cagttgagca
     8341 gccccccgcc gagccggctg caaatcgcct cgacgccctt gcgattgggg catttttcgg
     8401 cgcggatcgt gaagccgttt gggaacctct cctcggctcc atccaccgga atgaagccaa
     8461 ccgccagcac ggccgcggga tcgatcgcgc gaacgtactt ggcgagcagg tatgcctctt
     8521 ccaccgtaag ctgcggcgaa atcacggccg ccagtcgccc ggctttccgc agtttttgat
     8581 caatctcggc cgcgaattcc gaccactcga cgttctcgta ggcgtccttc acgcgccacc
     8641 gcagctgcgt caggcgatcc gcgtcgtgca cgtgcttcca gccataccgg ccttcgtcgc
     8701 acatccacca ctggttgacg tgggggttct cgcggggctt gaggcgatag acctggtcct
     8761 ggttttcttc gattttgatc gagcagcccg tggagcagcc ggcgcacacg
//
LOCUS       DASRJB010000054         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_865818, whole genome shotgun sequence.
ACCESSION   DASRJB010000054 DASRJB010000000
VERSION     DASRJB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865818"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..453)
                     /locus_tag="VFB80_01520"
     CDS             complement(103..453)
                     /locus_tag="VFB80_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32458.1"
                     /translation="MKGSVLVAGKPAARATVIFHPKAPAADALAEKAPPRPTGEVQAD
                     GSFTLSTYTAGDGAPAGEYTVTISWPSGSSPIGGDADSGPDRLGGRYLNPQTSRLSAK
                     VDAAATEVPRFELK"
     gene            complement(691..1689)
                     /locus_tag="VFB80_01525"
     CDS             complement(691..1689)
                     /locus_tag="VFB80_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN32459.1"
                     /translation="MRHSTFAFGLLVALSAGRPLPAAENETYAGYRTTTLEYKAPDGS
                     ERKRSVTLWYPTAAKAERHSYRPQVGFVAADADVLGRKNPLILFSHGFLGAADQTIFL
                     TEELARQGYIVAGVNHADSLRERSDGPQPRPNFAQPQTWDDTKYRDRRDDVVALLDHL
                     LALSGEKDSFLFQRVNPQQVGALGHSLGGYTVLGLAGGWKAWHEPRIKATVALSPYSL
                     PYVKSGKDNVKAINIPIMFQGGTLDIGVTPFLPPLYDQLAAPKYLLTLKNENHFGWTN
                     FASFTKTTTDTVQQGNPQLITDYTVAFFDHHLRRLPAAELLTKENERLEAFKYAAK"
     gene            1881..2504
                     /locus_tag="VFB80_01530"
     CDS             1881..2504
                     /locus_tag="VFB80_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZN32460.1"
                     /translation="MPYTLPPLPYAYNALEPHIDARTMEIHHTKHHQAYINNVNGAIA
                     GTELENLPVEKLIADLGKAPEDKRMVVRNNGGGHANHTLFWTILGPKAGGSPAGDLAA
                     AISGSFGSFDTFKEQFGKAATTRFGSGWAWLVVQGGKLAVGSTANQDSPLMGQAVAGI
                     AGTPILGLDVWEHAYYLNYQNRRPDYITAFWNVVNWNEVAKRFAAAK"
BASE COUNT          519 a          959 c          927 g          521 t
ORIGIN      
        1 gccgatgatg gcgatcacga cgagcagctc aaccagcgtg aagcccccgc gccggcccgg
       61 cgcggggaaa ggctcagcaa tcatcaaggg cgcctcctca ggttacttga gttcaaaacg
      121 cgggacctcg gtggccgcgg cgtcgacctt cgcgctcagg cgggaggtct gcggattgag
      181 atacctgccg ccgagccggt ccgggccgga atcggcgtcg ccgccgatcg gcgagctgcc
      241 cgacggccag gaaatcgtta ccgtgtattc cccggccggt gcgccgtcgc ccgccgtgta
      301 cgtgctcagc gtaaagctcc cgtcggcttg cacttcgccc gtcggtcgcg gtggcgcttt
      361 ttcggccagg gcatccgcgg ctggggcctt ggggtggaag atgaccgtcg ctctggcagc
      421 cggctttccg gccaccagga cgctgccctt cacggggaaa atcggaactc tttgctcctt
      481 tgtcccgcag ccccaagccg acagtgcgca ggccagaatc caacagccag cgccgaccct
      541 aatcctgcac atccttttct cacttccacc cccgcgagcc ccaattgtac ttcgttttgt
      601 tggctttgtc aggcaccgat gcgccgcttt gccagcggcg tccggcgctc agtttctcgc
      661 tcaactccta caagctctgg cgaattcgag ctatttggca gcgtacttga acgcctccag
      721 tcgctcgttt tccttggtca gcagctcggc ggccggcagg cggcggaggt gatggtcgaa
      781 gaacgcgacg gtgtagtcgg tgatcagctg cggattgccc tgctggacgg tgtccgtggt
      841 ggtcttggtg aagctggcga aattcgtcca gccgaagtgg ttttcgttct tgagcgtcag
      901 cagatacttg ggcgcggcga gctggtcgta gagcggcggc aggaacggcg tgacgccaat
      961 gtcgagcgtg cccccctgga acatgatcgg gatgttgatt gccttgacat tgtccttgcc
     1021 gctcttgacg tacggcagcg agtagggcga cagcgcgacc gtggccttga ttcgcggctc
     1081 gtgccacgct ttccagccgc cggccaggcc gagcaccgtg tagccgccga gcgagtgccc
     1141 cagcgcgccc acctgctgcg gattgacccg ctggaagagg aacgagtcct tctcgccgct
     1201 cagcgcgagc aggtggtcga gcagtgcgac gacgtcgtcg cggcggtcgc ggtacttcgt
     1261 atcgtcccac gtctggggtt gagcgaagtt gggccgcggc tgcggcccgt cgctgcgctc
     1321 gcggagcgaa tcggcgtggt tgacgcccgc gacgatgtag ccctgccggg ccagctcttc
     1381 ggtcaggaag atcgtctggt ccgccgcgcc cagaaagccg tgcgagaaga ggatcagcgg
     1441 gttcttgcgt cccaatacgt cggcatccgc cgcgacgaag ccgacctgcg ggcgatagct
     1501 gtgccgctcg gccttggccg ccgtcggata ccagagcgtc acactgcgtt tccgctcgct
     1561 gccgtccggc gctttgtatt cgagcgtggt cgtccggtag ccggcgtaag tttcgttctc
     1621 ggccgccggc agcgggcgac cagcagacag ggcgacaaga agtccgaaag caaacgtcga
     1681 atggcgcatg gcggcggcct cgagcggacg cggattacat cgatttaacc ccggaattgg
     1741 cccgagagtt tcacacgacc accttcggca cgtgcttcgc ggcgagtggc ccgattgcga
     1801 ccttcgccaa gctggagtat cttcgcgcga agctgaccac cactcaccac cgaccactca
     1861 ccactcactg gagttacccc atgccctata ccctccctcc cctgccctac gcctacaacg
     1921 ccttggagcc ccacatcgac gcgcggacga tggagattca tcacaccaag caccaccagg
     1981 cgtacatcaa caacgtcaac ggcgcgatcg cggggacaga gctggagaac ctgccggtcg
     2041 agaagctcat cgccgacctg ggcaaggcgc ccgaggacaa acggatggtc gtccgcaaca
     2101 acggcggcgg ccacgccaac cacaccctgt tctggaccat cctggggccg aaggccggcg
     2161 gctcgccagc gggcgacctg gccgcggcta tcagcggctc gttcggcagt ttcgacacct
     2221 tcaaggaaca gttcggcaag gcggcgacca cgcggtttgg cagcggctgg gcctggctgg
     2281 tcgttcaggg gggcaagctg gccgtcggtt ccaccgccaa ccaggacagc ccgctgatgg
     2341 gccaggccgt ggccggaatc gccggtacgc cgatcctggg cctcgacgtc tgggaacacg
     2401 cctactacct caactaccag aaccgccgcc cggactacat cactgcattc tggaatgtcg
     2461 tgaactggaa cgaagtcgcc aagcggtttg cagcggcgaa gtagcggcgg gggcgcccta
     2521 aacgaacgcc ggcgagggta aggataattc cgcagagcgc gagtttcttt cgcgcccctc
     2581 tgcgccgcca gctcgccaca actgcctgcc agttgaggac ttccggcgga atgccggcgg
     2641 ttggcggcca ttgcgaacat aagcactaga gagaccgacc gggaattatc cttaccctat
     2701 tggaggtgaa cgtagcggcg gcaattctgc cccagatgcc gacgacccaa ttgcgagaga
     2761 gcgtaatcgg ctggcagcgg gcagccaaaa tggcacccgc cgcgccaacg ggaactatac
     2821 tgcaaatagc ataccaatgt tcactgctcg ctaagtcccg cggccgcgtt tttcttccgc
     2881 gtagctcgtt cgcaggtaga ccggagccag cttccagaaa tcgtcc
//
LOCUS       DASRJB010000055         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2557752, whole genome shotgun sequence.
ACCESSION   DASRJB010000055 DASRJB010000000
VERSION     DASRJB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557752"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFB80_01535"
     CDS             complement(<1..415)
                     /locus_tag="VFB80_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32461.1"
                     /translation="MVPRRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMKCAN
                     NLRQIGLAIHNHEDTYKYMPPGIVQTPTTTPIPGLDDYLATTPKTPARLYANHGFLSI
                     MLPYLEQANVLVNAGGGYNFHLDWDEGANRTAAAIR"
     gene            604..963
                     /locus_tag="VFB80_01540"
     CDS             604..963
                     /locus_tag="VFB80_01540"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HZN32462.1"
                     /translation="MAAAATTPTVEMHFEKSAPEVRATYEAILKASRALGKVAIEPKK
                     TSIHLVRESAFAGIATRKDALILTLKSAAEIRSRRVAKAEQASAKRWHMEFRLTSPAE
                     VDRELREWLERSWELSD"
     gene            complement(1062..1880)
                     /locus_tag="VFB80_01545"
     CDS             complement(1062..1880)
                     /locus_tag="VFB80_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32463.1"
                     /translation="MTGTVASTQTESQTTNYFSNYLPPADPNRIYRVVGFMEYSKALE
                     SLDALAALATNYELAAIDEIVKSEPEFTIYTNLARRLERGRSNLAEKPAGENDDFARK
                     GFRWVTALARVEVGAMLAGFSSHPQPFAAVAGTGYDLAEYKELLLDGAKMHFWALTND
                     PGFTAIMRKFNPSGTTLAYLRRLNVASAFVSALVASAGREFNPAQVQEVKLYATKLEG
                     SRGDIRLSIENYLQQVMASTKTNLVGRQDNILLEACYKNVCQQYSRDVAALPRQ"
     gene            complement(1899..3194)
                     /locus_tag="VFB80_01550"
     CDS             complement(1899..3194)
                     /locus_tag="VFB80_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32464.1"
                     /translation="MSPIGLRAGVFSLLAVAAASLSTALAQESPPAKSAQPAAKAAQP
                     PARLTPKQLAFVKNELLPAWRAGDPLHLLRLVGEQVAKQSEQQNAALDRALAEEKAPS
                     LGRMLTDARMNLVRLDAGSQAPEATLAEIVLMVGELERQIQEKIATAKKVELFADPLP
                     SPATLTEFRDQLWKAHVQNNELINAAFLALQGRALLNSPLIAKVKNPTPGQEAAFAID
                     FVKYQGEAQELQRDLNERAIELRVNRIAFALKLLEGKNDLKERFLAAYAVGVDGEILI
                     QGLKNDKVRFRREKLSSPTLADELRASVDRGKTLAGDLIKKSELLFAGLHWWRRGRYG
                     RGSELHGLLKPEAALNNPAAQIGLLMPRTSPAPTDPAKGGRPSPDFDRRHHWTWAWED
                     RQFETVGTGSHTSEPVTKLVSTETQRMRHWDGIVDDRFY"
BASE COUNT          552 a         1033 c         1091 g          599 t
ORIGIN      
        1 tccggatggc ggcggcggtc cgattcgcgc cttcatccca gtccaagtgg aaattgtagc
       61 cgccacccgc attgaccagc acattggcct gctcgagata cggcagcatg atcgacagaa
      121 agccatgatt ggcatagagg cgagcgggag ttttgggcgt tgtggcaagg tagtcgtcca
      181 aacctggaat tggcgtggtg gtaggggtct ggacgatccc gggcggcatg tacttatacg
      241 tgtcctcgtg attgtgaatg gccaggccga tctgccgcag attgttggcg cacttcatgc
      301 ggcgggcggc ttcccgcgcg gcttgcacgg cgggcaacag cagggccacc agaaccccaa
      361 tgatggcgat gacgaccagc aactccacca gcgtaaaagc acgcctggga accatagaga
      421 aacctctcgg aaacgggcca atggcggact gtggcgcaga ccgccacaga ctcctcgtgc
      481 tgagggagag atcaagcgca gctgattgtg cgtggactcg gccagatcgt caagcaatcg
      541 gtgttaagat ttggttattg ccggaatgtg cccgcaacat gctcgtgaca atggggggct
      601 gcgatggcag ctgcagcgac gacgcctacg gtcgagatgc acttcgagaa gtcagcgccc
      661 gaggtgcggg ctacgtacga ggcgatcctc aaggcgtcgc gggcactggg aaaggtggcc
      721 atcgagccga agaaaacgtc gattcacctg gtgcgggaaa gcgccttcgc cggcattgcg
      781 acgcgaaagg acgcgctcat tctgaccctg aagtcggcag cggaaatccg cagccggcgg
      841 gttgcaaagg ccgagcaagc gtcggccaaa cgctggcaca tggaattccg gttgacctcg
      901 cccgcggaag tggaccgcga actacgcgaa tggcttgagc ggtcctggga gctgtccgac
      961 tagaaggtcc cgcctgccgg gcgggacctc ggatctttca acgtcgctgc gcttccctca
     1021 aggccccttc cggcagaagg ggcctacctg gctgaagggg cctactgtct cggcagggcc
     1081 gccacgtcgc ggctgtattg ctggcagacg ttcttgtagc aggcctccag caggatgttg
     1141 tcctgccggc ccacgaggtt cgtcttcgtc gaggccatca cctgttgcag gtagttctcg
     1201 atcgacaggc gaatgtcgcc gcggctgccc tcgagcttcg tggcgtagag cttcacctcc
     1261 tgcacctggg ccgggttgaa ctcgcggccg gctgaggcga ccagcgccga gacgaaggcc
     1321 gaggcgacgt tcagccgccg caggtaggcg agcgtcgtgc cgctcgggtt gaacttccgc
     1381 atgatggccg tgaagcccgg atcgttggtc agggcccaga agtgcatctt cgcgccgtcc
     1441 aggagcagct ccttgtactc ggccaggtcg tagcccgtgc cggccaccgc ggcgaacggc
     1501 tgcggatggc tgctgaaccc ggccagcatc gcccccacct cgacgcgggc cagcgcggta
     1561 acccagcgga agcctttgcg ggcgaagtcg tcgttctcgc cggcgggctt ttcggccagg
     1621 ttcgagcggc cccgctccag ccgccgcgcg aggttcgtat agatcgtgaa ttccggctcg
     1681 gacttcacga tctcgtcgat cgcggctagc tcgtagttcg tggccaaggc cgccagcgcg
     1741 tcgaggcttt ccagggcctt ggaatattcc atgaacccga cgacgcggta gatccggttg
     1801 ggatcggccg gcggcaggta gttgctaaaa tagttcgtcg tttgcgattc ggtctgggtg
     1861 gatgcgaccg taccggtcac cccaggcggc agccgcactc agtagaagcg gtcatcgacg
     1921 atgccgtccc agtgccgcat ccgctgcgtt tcggtcgaga cgagcttggt gacgggctcg
     1981 ctggtgtggg agccggtgcc gacggtctcg aactgccggt cctcccaggc ccaggtccag
     2041 tggtggcggc ggtcgaagtc gggcgagggc cgcccgccct tggccggatc ggtcggcgcg
     2101 gggctcgtgc ggggcatgag cagcccgatc tgggcggcgg gattattcag ggcggcctcg
     2161 ggcttgagca ggccgtgcag ctcggagccg cggccgtagc ggccgcgccg ccaccagtgc
     2221 agtcccgcaa agagcagctc gctcttcttg atcaggtcgc cggccagcgt cttgccgcga
     2281 tcgacgctgg cccgcagctc gtcggccagc gtcggcgagc tgagcttctc gcggcggaac
     2341 cgcactttgt cgttcttcag gccctggatc aggatttcgc cgtccacgcc cacggcatag
     2401 gccgccagga accgctcctt caaatcgttc ttgccttcca gcagcttcag ggcgaacgcg
     2461 atgcggttga cgcgcagctc gatcgcccgc tcgttcaagt cccgctgcaa ttcctgggct
     2521 tcgccttgat acttcacgaa gtcgatggcg aaagccgcct cttgacccgg cgtcggattc
     2581 ttcaccttcg caatcaacgg cgaattcaag agggcccggc cctgcagcgc cagaaacgcc
     2641 gcattgatca gctcgttgtt ctggacatgg gccttccaga gctgatcgcg gaattccgtc
     2701 agcgtcgccg ggctggggag cggatcggca aacagctcga ccttcttggc cgtggcgatc
     2761 ttttcctgaa tctgccgctc gagctcgccg accatcaaca cgatctcggc gagcgtcgcc
     2821 tcgggggcct ggctgccggc atccagccgc accaggttca tccgggcgtc ggtcagcatc
     2881 cggcccaagc tcggcgcctt ttcctcggcc agcgcccggt cgagcgcagc gttctgctgc
     2941 tccgattgct tggcgacctg ctcgcccacc agccgcagca ggtgcagcgg atctcccgcg
     3001 cgccaagccg gcagcagttc gttcttgacg aaggccaact gcttcggcgt cagcctggct
     3061 ggcggctgag cagctttggc tgcgggctgc gcggatttgg ccggcggcga ttcttgtgca
     3121 agagcggtcg agagactggc cgccgcaact gccaagaggg agaaaacgcc ggcgcgcagg
     3181 ccaatgggcg acatgttcgc acccttctct attcgagagg ctgccgcccc ggttgcagcc
     3241 ggagggtatt gctacccacc actcgcatgg tagga
//
LOCUS       DASRJB010000056         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1712259, whole genome shotgun sequence.
ACCESSION   DASRJB010000056 DASRJB010000000
VERSION     DASRJB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712259"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFB80_01555"
     CDS             complement(<1..221)
                     /locus_tag="VFB80_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN32465.1"
                     /translation="MPANAIKVARPDHPVLPVIAERWSPYAYDPRPVEREKLLSCLEA
                     ARWAASSYNEQPWTYILAERADAAEFAKVL"
     gene            588..959
                     /locus_tag="VFB80_01560"
     CDS             588..959
                     /locus_tag="VFB80_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN32466.1"
                     /translation="MIHLDSNFLIDAFVPGTAQETQFRQWIATAAALNLSAVAWGEFL
                     CGPLTPAAENLARTLFPVPESFGASDAELAATLFNVGGRRTRSFADCCIAAVAIRCGA
                     GLATSNRGDFAPFAAQGLIFA"
     gene            complement(971..1528)
                     /locus_tag="VFB80_01565"
     CDS             complement(971..1528)
                     /locus_tag="VFB80_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32467.1"
                     /translation="MKQTSPLDLIQIASPCHVSWDEMTGDERTRFCRHCKLHVYNISE
                     MDRAEAEGFIASREGRACVRLFQRADGTVLTRDCPVGVRYLRQRFARAVAAVAGVCVA
                     LLSGTLFGGALSRLAQGAIKAPADAFASWIDPQPMQFQMVMGEICLSNPPPSAPPGSV
                     QDTAETPLPQPTPEQLQAIQQRLEQ"
     gene            complement(1525..2748)
                     /locus_tag="VFB80_01570"
     CDS             complement(1525..2748)
                     /locus_tag="VFB80_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32468.1"
                     /translation="MDYRRASRIAEWTLAAAVVLLHLAIFLWPEAGGQPQFLIQAAHD
                     AQLLSGIVLIVGWCILGPGSPWLRGGALPVLAILWFLPWNERIMPRETTAAFQFTLAI
                     TAAAVIVLLRLLGFRMQRVDPAAGPERSAQFSLFGLLIATTLIAAAIGGLEALRPAMT
                     QDRSPDFLFVLGELPRRPQQVREIVLAAAVALSAIGGLWVVARPGAAWFRLGGIALAL
                     PVAAVYLAYMTGEADQRTSVAVNLGLGLALVAGLTGLSVLPLRMLDFRLKRPAAAGAR
                     SVAAAPKLGQLASRGAAIAVVVALVGLLPFSKPLTRSLMGGLSPPATAFAAWIQPRDR
                     LALQKSYRLRVVQSLRLRSIRLGFELKQPASPLPPRPLEIPSFREIDGWEYRTYSGPL
                     MDGRSIIRSTGEDEP"
BASE COUNT          554 a          935 c          953 g          468 t
ORIGIN      
        1 agcaccttgg caaactcggc ggcgtcggcc cgctcggcca ggatgtaggt ccacggctgc
       61 tcgttgtagc tcgacgctgc ccaccgcgcc gcctccaggc acgagagcag cttctcccgc
      121 tccacgggcc gcgggtcata ggcgtacggg ctccaccgtt cggcaatgac cggcagcacg
      181 ggatgatcgg gcctggcgac tttgatggca ttggcgggca tagactcctc cgtttctgat
      241 tcgcaccagt aggcagataa actagaatgg agagaggtga aggtacgcta ttctagccgg
      301 agcaggagtg gaggactctc gtgacttgcc tcatccccaa agagttggag gagcgcttgg
      361 cggcgcgtgc cttggagcgc caaacgacgg tcgaccaaat cgtatccgaa gcgatatcga
      421 gcttcttggc aagaactgaa gcaaggagtg cggaggagca ccaagccgat cggttgcgga
      481 cctatcgcga gttgcagcag gccctggcgc tgacgccgca aaaggcagcc gagtggaaag
      541 cggcgatcca agatggccgg cggtaatgct tgctgtacgc ctcggccatg attcacttgg
      601 attcaaactt tttgattgac gcctttgtgc ccggcaccgc gcaagaaact cagttccgac
      661 aatggatcgc gaccgcggcg gcgctaaatc tctccgcagt tgcctgggga gagttccttt
      721 gtgggccact gacgccagcg gccgagaatc tggcgcgaac tctcttcccc gtgccagaat
      781 cttttggcgc gtcggatgct gaattagcgg cgacgctctt caacgtcggc ggccgacgta
      841 ctcggagttt cgcggattgt tgcatcgctg cggtcgccat tcgttgcggc gcaggcctgg
      901 caacgagcaa ccgaggcgac tttgcccctt ttgcggcaca aggcttgatc tttgcataag
      961 ccgccggggc tcactgctcc agccgctgct gtatcgcttg cagctgctcg ggcgtcggct
     1021 gcggcagtgg cgtttcggcc gtgtcttgca cagagccggg cggagcggag ggcggcggat
     1081 tggacaggca gatctcgccc atcaccatct gaaattgcat cggctgcgga tcgatccagg
     1141 aggcaaaggc gtcggccggg gccttgatcg cgccctgggc cagccggctg agcgctccgc
     1201 cgaagagcgt gccgctgagc aaggccacgc aaacgcccgc cacggccgcg accgctcggg
     1261 caaaccgctg ccgcagatag cgcactccga ccgggcaatc gcgcgtgagc accgtgccgt
     1321 ccgcgcgctg aaacagtcgc acgcaggcgc ggccctcgcg gctggcgatg aagccttcgg
     1381 cctcggcgcg gtccatttcg ctgatgttat agacgtgcag cttgcagtgc cggcaaaacc
     1441 gcgtccgctc gtcgccggtc atttcgtccc acgacacatg gcagggcgag gcgatctgga
     1501 tcaggtcgag tggagaagtt tgcttcatgg ctcatcctcg ccggtcgagc gaattatcga
     1561 gcggccgtcc attagaggcc ccgagtaggt cctgtattcc caaccatcaa tctcccgaaa
     1621 tgacggaatc tcaagtggcc gcggcggcaa gggcgaagcc ggctgtttca attcgaagcc
     1681 taaccggatc gatcgcagcc gcagcgactg gactacacgc aatcggtagg atttctgaag
     1741 agccagccgg tctcgcggct gaatccaggc ggcaaaggcg gtggccggcg gcgaaaggcc
     1801 gcccatcaag ctgcgggtga ggggcttgga aaatggcagg agacccacga gcgctacgac
     1861 gacggcaatc gccgccccgc gcgacgccag ttggccaagt tttggcgcgg ccgcgacgct
     1921 gcgagcaccg gctgctgcgg gccgcttcaa gcgaaagtcg agcattcgca gcggcagcac
     1981 gctcaagcca gtcaaaccgg ccaccagggc cagccccagg ccgaggttga ccgccacgct
     2041 cgtccgctgg tccgcctcgc cggtcatata cgccaggtag acggcggcca cgggcaacgc
     2101 cagggcgatc ccaccgagcc ggaaccacgc tgcgcccggc cgtgcgacga cccacagccc
     2161 gccgatcgcc gacagcgcca cggcggccgc cagcacgatc tcgcgaactt gctgcggcct
     2221 gcgggggagt tcgcccagta cgaacaaaaa atcgggtgag cggtcttggg tcatcgccgg
     2281 ccgcagggcc tcgagcccgc cgatggcggc ggcaatcagt gtggtcgcga tcagcagccc
     2341 gaatagggag aattgagcgc tccgctcggg tccggctgcc ggatcgaccc gctgcatgcg
     2401 gaagcccagc agccgcagca ggacgatgac cgccgcggca gtaatcgcca aagtgaattg
     2461 aaatgcggcg gtcgtttccc gcggcatgat ccgctcgttc cagggcagga accagaggat
     2521 cgccagcacg ggcaaagccc cgccccggag ccagggagag cccggaccca aaatacacca
     2581 gccgacgatc agcacgattc ccgacagcag ctgcgcgtcg tgagccgcct ggatcagaaa
     2641 ctgcggctgc ccgcccgctt cgggccacag gaagatggcc aggtggagca acacgaccgc
     2701 ggccgccagc gtccactcgg cgatgcggct tgcgcggcgg tagtccacgg cggccctccg
     2761 gtgataagac gccccgcaat cggccctggt tctgcaggtt ttcgcagtat aatcgacaga
     2821 aatcacacgc tcttatgttc cggatcaagg tctgcggcat cacgacgagc gacgacgcgc
     2881 tgctagccgc cgagtgtggc gcggacgcga
//
LOCUS       DASRJB010000057         7740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_867323, whole genome shotgun sequence.
ACCESSION   DASRJB010000057 DASRJB010000000
VERSION     DASRJB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867323"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /locus_tag="VFB80_01575"
     CDS             complement(<1..2325)
                     /locus_tag="VFB80_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HZN32469.1"
                     /translation="MAGQGRPQFRARRLARGLVPLALAILIPAAAAAGDVDARIRLAW
                     GGGEARAWQGAIQISEGTLSEVTPLGLEADAPGSLLPVDPRTVRVFPRVPRNYDCCDL
                     RVQAPENARLLVQLWTAGSPPPAPVEVPLAKVLRGFSQFELDDRKNRLVVQRSPGDAL
                     RVVVRRDTLIFAPEEPLELEIQPSVPDLTPGATYLLATSLETARLDEQLWNKDVDVRV
                     DSSGRAAAVQLSVPLPAAEGVYDVKLYLYPKRLTSSLVRGKPLASRRVQLVVVEPVKM
                     LDRGNAAWQSLLEFDPTSPKWWERLAPRLPAWTGLPSLPQPVESGPAKVKSHQSRSWV
                     ELPPKAWQAYPLSVSAPGTPHILEVEYPSDIEQTLAISLVEPNVAGQVGPIGLDSGVD
                     VPAPHPGHQPQIRKHRLVCWPQTKTPYVLVVNRRDDRSALVGRIELLAGPRELPPAKI
                     PQPNFPTRLLAAYYDKPLVAENFSATEALDPVSRRHLDDWTTFYEAGQRLVQTLQHGG
                     YNALVLTVACEGSAIYPSRLLEPTPRYDTGVFFESGQDAIRKDVLELLMRLCDRSGIQ
                     LIPAVQFSGPLPQLEALRKAGGPAAVGLEPIGPDGRTWLARRNLAGSVQRDPEGGLGV
                     WYNALDPRVQQAMQAVVGELSDRYAHHPCFGGVAVHLSSESYALLPDETGSFDDATYA
                     RFLEETQTQPPQFAADLAPLAAREQFLCGEGEAAWIKWRTERLTKMYAQMHRDIARRR
                     GGARLYLTTADLLGGRQIQQALRPTLPPRPEPHRL"
     gene            complement(2462..3664)
                     /locus_tag="VFB80_01580"
     CDS             complement(2462..3664)
                     /locus_tag="VFB80_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN32470.1"
                     /translation="MLSRHVVVVVIDRLGAGWLGPYGNTWLETPHFNRLAASALLCET
                     AIADSPELLAAYRSYWTGRHAFAPDGPDAESLPKLASAEGHKTILVTDDGRIAAHPQS
                     ADFGEIRRVSAEPRAAAATSIDGTGLFQLTTAALEALAESAERSLVWIHSRGMDGPWD
                     APLELRNQFADEDDPEPPQLVQPPALVLPENYDPDALLGLTQAYAGQVALADECLGLL
                     LEVLGERRPDETLLIVTSPRGYPLGEHRRVGDADPALYAELLQVPLLVRFPATVGALV
                     RAQTIVQPQSVYATIVGAKGSGDLASLVAGESLPGGEAAVSVAEGQRSIRTPAWFLRE
                     SQRERETERELYAKPDDRWEANEVASRCGETADLLAARMDEFQSAARAGSLAGLPPLT
                     QVLADGWR"
     gene            complement(3669..4292)
                     /gene="lexA"
                     /locus_tag="VFB80_01585"
     CDS             complement(3669..4292)
                     /gene="lexA"
                     /locus_tag="VFB80_01585"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZN32471.1"
                     /translation="MASSLLDQLTSRQAAVFEFIREKVQTRGYGPTVREIGEHFQISS
                     PNGVMCHLRALERKQLLRRVRKHDRAIARAIELNPEIMAEDKGMPLVGTVAAGLTTLA
                     WEQGDRVDFGQMFNKKNLFSLKVKGDSMIEAQIADGDYVVCKKQKTAQPGQMVVAQTP
                     EGEATLKYWHPEANRIRLQPANSSMQPIFVKDASVVGVVVGVVRNVN"
     gene            4609..5856
                     /gene="proB"
                     /locus_tag="VFB80_01590"
     CDS             4609..5856
                     /gene="proB"
                     /locus_tag="VFB80_01590"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZN32472.1"
                     /translation="MSVGSFLPTPPAAIRPATPRPHVPHLASAVTEKSPSAADMRQPV
                     IAGAHTVVVKVGTRVLTQADGTLDLPRIELLAEEIHTISSGGRRVVLVSSGAVGAGLS
                     LLGLKGRPTDLAKLQAVAAVGQTHLIQAYDETFARHGRHAAQVLLTLEDVDDRVRYLN
                     VRNTLLSILEFGAIPVVNENDTVSVDELKTTFGDNDRLAAMVTNLIRAPLMIVLSDIE
                     GLYDGDPGLKASKLVSTVERIDEQVLAYVRDRKTGLSKGGMSSKLEAARIVTSAGENM
                     IIASGRRTDVLTQLMAGEPLGTLFLAQGKGISPFKRWLGFSAQVRGRIQLDEGARHAI
                     LGKGRSLLAAGIVGTQGEFQKGDAVALCDNEGTVVARGLTNYSSAEIERIKGLKSEKI
                     AQVLGSRPYEEVIHRDNLAIMPK"
     gene            6129..6674
                     /locus_tag="VFB80_01595"
     CDS             6129..6674
                     /locus_tag="VFB80_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZN32473.1"
                     /translation="MKCQQCEKPATFHITELTGQEPQELHLCEGCAKSYLMKDESATP
                     VAPTLANVLAKQLQIGKAAEELAKLDQRACPVCGITFFEFRNQGRLGCPHDYVFFERE
                     LTPLIANIHGESRHVGKHPPGHAGASDQQTELIRLRREMEDAKTKEHYERAAKIRDQI
                     RELEKQVKPKNRPQAEGQGTA"
     gene            6689..>7740
                     /locus_tag="VFB80_01600"
     CDS             6689..>7740
                     /locus_tag="VFB80_01600"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HZN32474.1"
                     /translation="MSTDKLNDLAIRCGEWLRGSGPQSEIVMSSRIRLARNLAEYPFI
                     RRCTESDRLAIEKSFQDAIGSVGDWKSLLFCDVAALPTVDRQLLVERQLISRELSETN
                     GARCVYIDPQEQFSLMVNEEDHLRMQVMHSGLDMETAWEQINKMDDLLGERLNFAFHE
                     RLGYLTACPTNVGTGMRVSVMLHLPALVITQQIEKVFRSLQKMGLAVRGLYGEGSQAM
                     GDFYQISNQITLGRSELDLVKQVGEVIPVIIDYERQARAFLVKESRKDLHDRVSRAHG
                     ILCTAQTISSEETLHLLSSVRMGVYLGLIQDLEIPTINKLFIHTQPAHLQKLRGAELP
                     SADRNVERAMYLQAHLR"
BASE COUNT         1373 a         2563 c         2542 g         1262 t
ORIGIN      
        1 caggcggtgc ggctccggcc ggggcggaag cgtcggccgc agggcctgct ggatctggcg
       61 gccgcccagc aagtccgccg tggtgagata caaccgagcg ccgccgcgcc ggcgggcgat
      121 gtcacgatgc atttgggcgt acattttcgt cagccgctcg gtgcgccact tgatccaggc
      181 ggcctcgcct tccccacaga ggaattgctc gcgcgcggcg agcggggcga gatcggccgc
      241 gaactgcggc ggttgcgttt gcgtctcctc caggaaccgg gcgtaagtcg catcgtcgaa
      301 gctgccggtt tcgtccggca gcagggcgta gctctcgctg ctcaggtgca cggccacgcc
      361 gccgaagcag ggatggtgcg cgtagcggtc ggacaattcg ccgacgacgg cctgcatcgc
      421 ctgctgcacc cgcggatcga gggcgttgta ccagacgccg aggccgcctt ccgggtcgcg
      481 ctgcacgctg cccgcaagat tccgccgggc cagccaggtg cggccgtcgg ggccgattgg
      541 ctccaggcca accgccgccg gccctccggc ctttcggagc gcttccaact gcggcagcgg
      601 cccgctgaat tgcacggcgg ggatgagctg aatgccgctg cggtcgcaga gccgcatcaa
      661 tagttccaac acgtctttgc ggatcgcgtc ctgcccgctc tcgaagaaca cgccggtgtc
      721 gtaccgcggc gtgggctcca gcagtcggct gggatagatc gcgctccctt cgcaggcgac
      781 cgtcagtacc agggcgttgt aaccgccgtg ctgcagcgtc tgcaccagcc gctggcccgc
      841 ttcgtagaag gtcgtccagt cgtccagatg ccgccggctg accgggtcga gcgcttcggt
      901 cgcggagaaa ttctcggcca ccagcggctt gtcgtagtag gcggccagca gtcgcgtggg
      961 gaaattcggc tgcgggatct tcgccggcgg caactcgcgc gggccagcca gcagctcgat
     1021 acggccgacg agcgccgagc gatcgtcgcg gcgattgacg accagcacat acggcgtctt
     1081 cgtctgcggc cagcagacga ggcggtgctt gcgaatttgt ggctgatggc cgggatgcgg
     1141 cgcgggaaca tcgacgcccg aatccaggcc gatcggtccg acctgcccgg cgacattagg
     1201 ctcgaccagg ctgatggcca gcgtctgctc gatgtcgctg ggatattcga cctcgagaat
     1261 gtgcggcgtg cccggcgcgc tgaccgagag cgggtaggcc tgccaggcct tgggcggcag
     1321 ttcgacccac gaacgcgact ggtggctttt gacttttgcc ggcccgcttt ccacgggctg
     1381 aggcaagctg ggcaggccgg tccaggcggg cagccgcggg gccagccgct cccaccactt
     1441 ggggctcgtc ggatcgaatt ccaggaggct ttgccacgcc gcgtttccgc ggtcgagcat
     1501 cttgacgggc tcgacgacga ccagttgcac ccggcgggaa gcgagcggtt tgccgcggac
     1561 gagcgacgag gtcagccgtt tgggatagag atagagcttg acgtcgtaaa cgccttcggc
     1621 ggccggcaac gggaccgaca gctgcaccgc cgccgcccgg cccgagctgt cgacgcggac
     1681 gtcgacgtct ttgttccata gctgctcatc caggcgggcg gtttcgagcg atgtcgccag
     1741 caggtaggtc gcgcccggcg tcaggtcggg gacgctcggc tgaatttcca gctccagcgg
     1801 ctcctcgggc gcgaagatca gcgtgtcgcg gcgaacgacc acgcgcaggg cgtcgccggg
     1861 cgagcgctgg acgaccaggc gattcttgcg gtcgtcgagc tcgaactgcg agaagcctcg
     1921 gaggactttg gccaggggga cctcgaccgg cgcggggggc ggcgagccgg cggtccacag
     1981 ctgcacgagc agcctggcgt tctccggcgc ttgcacgcgc aggtcgcagc agtcgtagtt
     2041 tcgaggcacg cggggaaaga cgcggaccgt cctgggatcg actggcagca gcgagccggg
     2101 cgcgtcggcc tccagcccca gcggcgtcac ttccgacaga gtgccttcgc tgatttgaat
     2161 cgcgccctgc cacgcgcggg cttcgccgcc gccccaggcc aggcggatgc gcgcgtcgac
     2221 gtcgccggca gcggctgccg ccggaatcag gatcgccagg gccagcggca ccaggccgcg
     2281 ggcaagccgg cgagcgcgaa attgcggacg tccttgtccg gccatgttgc gcgcgaaaat
     2341 tcggctcacc gcgcaggctg cctcctgccg ccagcgcgcg gtgagattcg ccggggatta
     2401 tatgtctggg gccgctgcga cccctacaga gaattgcggc agggccacgc gccgcgcggc
     2461 cctagcgcca gccgtcggcg agaacttggg tgaggggcgg aaggccggca aggctacccg
     2521 cgcgggcagc ggactggaac tcgtccatcc gcgctgctag caggtccgcc gtctcgccgc
     2581 agcgggaagc gacttcgttt gcctcccagc gatcgtcggg cttggcgtag agttcgcgct
     2641 cggtttcacg ctcgcgctgc gactcgcgaa gaaaccaggc cggcgtgcgg atcgaacgct
     2701 ggccctcggc cactgacacc gccgcctcac cgcctggcag cgactcgccg gcgaccaggc
     2761 tggccagatc gccgctgcct ttggctccca cgatcgttgc atagacggac tgcggctgga
     2821 cgattgtctg cgcccgtacc agcgcgccta ctgtcgctgg aaaacgcacg agcagcggca
     2881 cttgaagcaa ttcggcgtac agcgccggat cggcgtcgcc gacgcggcgg tgctcgccta
     2941 gtggatagcc ccgaggcgag gtgacgatca gcagcgtttc gtcgggccgg cgctcgccga
     3001 gcacctccag caacaggccc aggcactcgt cggcgagcgc cacttgcccg gcataggcct
     3061 gcgtaagccc cagcagcgca tcgggatcgt agttttcggg caggacgagc gccggcggct
     3121 gcacgagctg cggcggctcc gggtcatcct cgtcggcgaa ctggttgcgc agttccagcg
     3181 gtgcgtccca cgggccgtcc atcccgcggg agtgaatcca aacgagcgac cgctccgccg
     3241 attcggccaa agcttccagg gcggcggtcg tgagctgaaa aagcccagtc ccatcgatgg
     3301 aggtggctgc ggcggcgcgc ggttcggccg aaacgcgccg aatctcgccg aaatccgcag
     3361 actgcggatg cgcagcgatc cgcccgtcgt ccgtgacgag gatcgtcttg tgcccctccg
     3421 ccgacgccag cttcggcagc gattccgcgt cgggcccatc gggcgcaaag gcgtgccgcc
     3481 cggtccaata actgcgatac gccgccagca gctcgggcga gtcggcgatt gccgtttcgc
     3541 aaagcagtgc cgaggccgcc agccggttga agtgcggcgt ctccagccac gtgttgccgt
     3601 acggcccgag ccagccggcg ccgagccgat cgatgacaac gacaaccaca tgccgggaca
     3661 gcatgctcct aatttacgtt ccggaccacg cccacgacca cgcccacgac gctcgcgtcc
     3721 ttgacgaaga tcggctgcat cgaggagttg gcgggctgca ggcggatgcg gttggcttcg
     3781 ggatgccagt atttgagcgt ggcctcgccc tccggcgtct gggcgacgac catctggccg
     3841 ggctgcgccg tcttctgctt cttgcagacg acataatcac cgtcggcgat ttgggcctcg
     3901 atcatcgagt cgcccttgac cttcaaggaa aacaggttct tcttgttgaa catttggccg
     3961 aaatcgacgc ggtcgccctg ctcccaggcg agcgtggtca ggccagcggc gacggtgccc
     4021 acgagcggca tgcccttgtc ttccgccatg atctcggggt tcagctcgat ggcccgggca
     4081 atcgcccggt cgtgcttccg cacgcggcgc agcagctgct tgcgctccag tgcccgcaag
     4141 tggcacatga cgccgttggg cgagctgatt tgaaagtgct cgccgatttc gcggaccgtc
     4201 gggccgtagc cgcgggtctg cactttctct ctaatgaatt caaacaccgc ggcctgccgg
     4261 ctggtaagct gatccaaaag cgaagacgcc atagcgatcc tcctgagtga acgaaactga
     4321 agtatcgtga attgtaatac atgggtgaac acggcggcaa ccacatttgt acagaaattc
     4381 tgaagttcag tggccaaaag ttggccttcc cccgacccac agatcaggag ttgtgccgta
     4441 acttgtgcaa cgccataggg ttatggctgg ctttgccggc gcaagactag gggttgcgga
     4501 aaaaccggtg aatctcctaa attcccgccc tggcgatcta ccgccagaca cggccgaccg
     4561 ttactcgctg ccggaccgcg cgtccacccc cgcaacctgg aagttatgat gtctgtcggt
     4621 tcgttcttgc ccacacctcc cgccgcgatt cgccccgcta cacctcgtcc ccacgtcccg
     4681 catctggcct ccgctgtgac cgagaaatcg ccctccgcag ccgacatgcg tcagccggta
     4741 atcgctggcg cgcacaccgt cgtggtcaag gtcgggacgc gggtgctgac acaagccgac
     4801 gggacgctcg atttgccgcg gatcgagctg ctggccgaag agatccacac catcagctcg
     4861 ggcggccggc gcgtggtgct ggtcagcagc ggcgcggttg gcgcggggct gagcctactc
     4921 gggctgaaag gtcggcccac cgacttggcg aagctacagg ccgtcgccgc cgtcggccag
     4981 acgcacctga tccaggccta cgacgaaacc ttcgcccggc acggccgcca cgccgcccag
     5041 gttctgctga cgctcgagga cgtggacgac cgcgtaaggt acctcaacgt gcggaacacg
     5101 ctgctctcga ttctcgaatt cggcgcgatt ccggtcgtga atgaaaacga caccgtcagc
     5161 gtcgatgaat tgaagaccac cttcggcgac aacgaccggc tggccgcgat ggtcaccaac
     5221 ctgatccgcg ctccgctgat gattgtgctc tccgacatcg aaggcctgta cgacggcgat
     5281 ccgggcctga aggcctcgaa gctggtctcg acagtcgaac ggatcgacga gcaggtgctc
     5341 gcgtacgtcc gcgaccgcaa gacgggccta agcaaggggg gcatgtcgag caagctggag
     5401 gccgcccgca tcgtcacctc ggccggcgag aatatgatca ttgccagcgg ccggcggacc
     5461 gacgtgctga cgcagctcat ggctggcgag ccgctgggga cgctcttcct cgcgcagggc
     5521 aagggcatca gcccgttcaa gcgttggctc gggttctccg cccaggtccg tgggcggatt
     5581 caactcgacg agggcgcgcg ccacgcaatc ctgggcaaag gccgcagcct gctggcagcc
     5641 ggcatcgtcg gtacgcaggg agagtttcaa aagggggacg ccgtggccct ctgcgacaac
     5701 gaaggcaccg tagtcgcccg gggattaacg aattattcct ccgccgaaat cgagcggatc
     5761 aaagggctca agtccgagaa aatcgcccag gtcctcggca gccgcccgta tgaggaagtg
     5821 atccaccgcg acaacctggc gattatgccg aagtaagtgc ggttctactc gcgctcgtca
     5881 tctgatatcc cgatcgccgc accgccgtcg tcggccgaac aggagcagca gcaggattaa
     5941 gattacgatt aggatttgga ttaggacgga agtgcttcgc cagccgcgtg gtctcctaac
     6001 cttgacactc cccccgccct tcctagaatc caatttggga agctaactct tgctgcgaaa
     6061 caacttgcac gccgtcgggt gaatcccgcc gcgggcaagg ccgaggccgc agcggcaagt
     6121 gggaacgtat gaaatgccag cagtgtgaga agcccgcaac gttccacatc accgagctga
     6181 ccggccagga gccgcaggaa ctgcacctct gcgagggctg tgccaagtcg tacctgatga
     6241 aggacgagtc cgccacgccc gtcgcgccaa cgctggccaa cgtgctggcc aaacagctgc
     6301 aaatcggcaa ggctgcggaa gagttggcca agctcgacca gcgggcctgc ccggtctgcg
     6361 gcatcacgtt ctttgagttt cgcaaccagg gccgcctcgg ctgtccgcac gactacgtat
     6421 tcttcgaacg tgagctgacg ccgctgatcg ccaacatcca cggcgagtcg cggcacgtcg
     6481 gcaagcatcc gcccggccat gcgggagcga gcgaccagca gaccgagctg atccgcctcc
     6541 gccgcgagat ggaagacgcc aaaaccaaag agcactacga acgggcggcc aagattcgcg
     6601 accagatccg cgaactagag aaacaggtca aacccaaaaa ccggccgcag gccgaaggtc
     6661 aaggaacggc ctagtcggcc tcacgaccat gtccacggac aagcttaacg acctcgcgat
     6721 tcgctgcggc gaatggctcc gcggctccgg cccgcagagc gagatcgtga tgtctagccg
     6781 catccgcctg gcccgcaacc tggccgagta tccgttcatc cgccgctgca ccgaaagcga
     6841 ccgcctggcc atcgaaaaga gctttcagga cgcgatcggg tcggttggtg attggaagag
     6901 cctgctgttt tgcgacgtcg cggccctgcc cacggtcgat cggcagttgc ttgtcgagcg
     6961 gcagctcatc agccgcgagc tgtccgagac caacggcgcc cgctgcgtct atatcgaccc
     7021 gcaggagcag ttcagcctga tggtcaacga ggaggaccat ctgcggatgc aggtcatgca
     7081 cagcggcctc gacatggaga ccgcctggga gcagatcaac aagatggacg acctgctcgg
     7141 cgagcggctg aacttcgcat tccacgagcg gctgggctat ctcaccgcct gcccgacgaa
     7201 cgtgggcacg gggatgcggg tgagcgtaat gctgcacctg ccagccctgg tcattacgca
     7261 gcagatcgaa aaggtcttcc gcagcctgca gaagatgggc ctggcggtcc gcggcctgta
     7321 cggcgagggc tcgcaggcga tgggcgactt ctaccagatc agcaaccaga tcacgctcgg
     7381 ccgtagcgag ctcgacctcg tcaagcaggt cggcgaggtc attcccgtca tcatcgacta
     7441 cgagcggcag gcccgcgcgt ttttggtgaa ggagagccgc aaagacttgc acgaccgcgt
     7501 cagccgggcg catggcatcc tctgcaccgc ccagaccatc tccagcgagg agacgctgca
     7561 cctcttgtcc agcgtgcgga tgggcgtcta tctggggctg atccaggatc tcgagattcc
     7621 tacaatcaac aagctcttca tccacacgca gcccgcccac ctgcaaaagc tgcgcggcgc
     7681 cgagctgcct tcggccgacc gcaacgtcga gcgggccatg tatctgcagg cccacctccg
//
LOCUS       DASRJB010000058         6131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2559094, whole genome shotgun sequence.
ACCESSION   DASRJB010000058 DASRJB010000000
VERSION     DASRJB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559094"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..423)
                     /locus_tag="VFB80_01605"
     CDS             complement(130..423)
                     /locus_tag="VFB80_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32475.1"
                     /translation="MRYRLRTLLILLAILPPLLAVSWWKYSEWKAEQERQRAERELVQ
                     MLRTGRAGIVGVQPPLPEPTMLQPTRRQPAFTPLPVTVPPDQPPLPLAPRETP"
     gene            complement(428..616)
                     /locus_tag="VFB80_01610"
     CDS             complement(428..616)
                     /locus_tag="VFB80_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32476.1"
                     /translation="MQYSLRMLVLLTAIGPPAIALVWFAWWPLLLLALCIAALTLWIA
                     GTLTICRFVAALFVSVMG"
     gene            complement(680..970)
                     /locus_tag="VFB80_01615"
     CDS             complement(680..970)
                     /locus_tag="VFB80_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32477.1"
                     /translation="MRYRLRTLLILLAIMPPLLWIGWTNYEAWKAEQERQAARERLLR
                     ERRDLTGIRIRLWQVNTGRLFFPTAPPQPMESPERAEAAERADAPAAPRGVR"
     gene            complement(970..1104)
                     /locus_tag="VFB80_01620"
     CDS             complement(970..1104)
                     /locus_tag="VFB80_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32478.1"
                     /translation="MFAWPHIEKFMPMWVCFGLTFAVVAAFLALLIVLASTARRQESD
                     "
     gene            complement(1190..2032)
                     /locus_tag="VFB80_01625"
     CDS             complement(1190..2032)
                     /locus_tag="VFB80_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32479.1"
                     /translation="MNLLVGLVAPAAIAWAAVWLLRRTLPAKTDDRGLLASAAAAAVF
                     IGYVLLPDWAALKPERHWQWLPYVGVAAAAVSALAPTSRGLWALRWALLAAAALIAAW
                     KLVPDWESLYPPRPISIVLVAAGMVALAAALEALPDRLCRPTLVALLAATAGGVMVAL
                     AVSVSLKFGQLAGLTASAIVGCAIACLRREAGPAVVRGLAPVAALLLVGVAYAGCIEP
                     DPPVNPLLFIPAAPLALWLCAAGPLSRLQGRPSIAAQVAVVAAPLLVAIIIACLSPPA
                     ADSW"
     gene            complement(2029..2619)
                     /locus_tag="VFB80_01630"
     CDS             complement(2029..2619)
                     /locus_tag="VFB80_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN32480.1"
                     /translation="MKRTLSLVVVLALALRAAAAEPVPVAGGKATLSPDNTRINFIGT
                     HAAPKKPDPRTGGFAKFKGQAAVDGKALKSVSVDIETESLWTEVGGRLTNHLKSADFL
                     EVREFPTAKFASTKIEPAADGKTKITGNLTLHGKTKEISFPATVAVTDKGLTLASEFT
                     IDRTDWGINYEPGQVVKQVTLKVAIGEKTQPTSGGR"
     gene            complement(2647..3693)
                     /locus_tag="VFB80_01635"
     CDS             complement(2647..3693)
                     /locus_tag="VFB80_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32481.1"
                     /translation="MLRVGLVGVGFMGWIHYLAYQKAKGVKLAAVCTRDRTRLSGDWR
                     GIQGNFGPPGEAVDLSEVAKYSALPALLADKSIDLVDLCLPPHLHAEATIAALKAGKH
                     VFVEKPMGLTAAECDKMVAAAKKAKRQVLVGHVLPFLPEYAFAWKAIESGKYGKLLGG
                     HFKRVISDPSWLKDFFNPDKVGGPLVDLHVHDAHFIRLVFGMPQAVTSQGRLRGGVVE
                     YCVSQFQFKNKSLVVSSASGVVNQQGRSFTHGFEIHLEKATLYFDLAVLAGGTLQITP
                     LTLLDSKGHAEQPKLPAVDPMLAAFDYEIQEVARSIASKKPSALLGGDLARDAIVLCH
                     KQTEAVKSGKTVRI"
     gene            complement(3786..5333)
                     /locus_tag="VFB80_01640"
     CDS             complement(3786..5333)
                     /locus_tag="VFB80_01640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HZN32482.1"
                     /translation="MPDASQVYLAVDLGASSGRVVAGLFDGTRLALEEVYRFDNGGVQ
                     AAGRMHWDVLNLWQQTLRGLKAAAKVYGSHVASVGVDTWGVDFALLSKSDELLSNPCH
                     YRDRRTVGILDKAFAIVPREEIFAATGLQFMEFNTLYQLLAMKQANSPLLQMAHSWLM
                     IPDLFHWLLTGVKANEYTNATTTQFLNPKTGKWATELLAKFGIRTDILGPIVQPGTKL
                     GKLRPEVAAETNLTTAEVILPGTHDTASAVAAVPAASRPLLPEGASGPARQAGPTVPD
                     WCYISSGTWSLMGVETPQPVINDRVYQLNFTNEGGIGGTTRLLKNIAGLWLIQECRRA
                     WKEAGRDYAWEELSRRASDRAPLAALINPDHPSFVAPENMPAAIVDFCTKTGQSAPVT
                     DGAIIRCALESLALRYRMVLGFLEELIGGRIETIHIVGGGAQNRLLCQMAADATGRRV
                     VAGPVEATAIGNLMVQAVACGAIKDIAQSREIIRTSFEVQEYRPKPSADWDEAYSRFE
                     QLVLAKG"
     gene            complement(5429..5932)
                     /locus_tag="VFB80_01645"
     CDS             complement(5429..5932)
                     /locus_tag="VFB80_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32483.1"
                     /translation="MPRYRVAVFPNNAASGATIGYYGTMLGICSTRRCSYWVYLPALD
                     DIIEVRGIDLLVVGPSALEPISEAEQEWLISERRWDVTFYTPIAEDNAELCGSYRIGT
                     RERGTFKFQKRDQSAPTWQFTIPTYASPCRAHLRYLIPARHALNRDTVMAALAAVLDV
                     EPPEDVA"
BASE COUNT         1111 a         2027 c         1956 g         1037 t
ORIGIN      
        1 cgccgtggca gggcgctaat cagattctat gcgccgtgcc gcagcccgca aggcgaaggg
       61 cgggaagggc ggatctttgc cacgaatcgg ccctgggcat cggacggtcg tcgcgtccag
      121 cgtagcagcc taaggcgttt cgcgcggagc aagcggcaaa ggcggctggt ccggcggcac
      181 agtgaccggg agtggggtaa acgccggttg cctcctcgtc ggctggagca tagtcggctc
      241 aggcagcggc ggctgcacgc cgacaatacc ggctcgaccc gttcgcagca tctgcaccag
      301 ctcgcgctcg gcccgctgcc gctcttgctc tgctttccac tctgagtatt tccaccagct
      361 caccgccaac agcggcggca ggatggccag caggatcaag agcgtgcgga ggcgataacg
      421 catgccgtca acccatgacc gaaacaaaca gggcggcgac gaaacgacag attgtcaatg
      481 tccctgcgat ccagagcgtt agcgcagcga tgcacaaagc aagcaacagc agcggccacc
      541 acgcgaacca gaccagagca atggcggggg gcccaatcgc tgtcagcagg accagcatgc
      601 gcaggctgta ttgcatgccc gcattttacc gcagcacgaa aaggccccgc cagtcggacc
      661 agcgatcggt cggtcgcgac taccgcacgc cccgtggcgc tgccggcgcg tcggcacgtt
      721 cggcagcctc cgcgcgctcg ggcgactcca ttggctgagg aggggctgtc ggaaagaaca
      781 gccttccggt gttcacttgc cacaagcgaa tgcgaatccc cgtcagatcg cgcctctcgc
      841 gcagaagtcg ctcccgtgct gcctgccgct cttgctccgc tttccacgcc tcgtaattcg
      901 tccagccgat ccacaacagc ggcggcataa tcgccagcag gatcagcagc gtgcggaggc
      961 ggtaacgcat caatcgcttt cctgccggcg ggcggttgac gccagaacga tcaagagcgc
     1021 gagaaacgca gcgaccaccg cgaacgtcaa tccgaagcag acccacatcg gcatgaactt
     1081 ctcgatatgc ggccaggcga acatgcttca aattctaccc gcacacgaaa tcgtcccgcc
     1141 agttgaacag cgggctggcc cgcaaggcga ggggcgggaa cggccgacct taccacgaat
     1201 cggcagccgg cggcgacagg caagcgatga tgatagccac cagaagcggc gcggctacga
     1261 cggcgacttg tgcggcaatc gatggtcggc cctgcagccg ggatagggga ccggcggcac
     1321 acagccacag cgccagcggc gccgcgggga tgaacagcaa tgggttgact ggcgggtccg
     1381 gctcgatgca gccggcgtag gcgacgccca ccagcagcag ggccgcgacg ggtgccagcc
     1441 cgcgcacgac cgcggggcca gcttcacgcc gcaaacaggc gattgcgcag ccgacaatcg
     1501 cgctggcggt cagcccggcc agttgcccga acttgaggct cacggaaact gccagcgcca
     1561 ccattacgcc gccggctgta gcggcaagca gggcgacgag cgtcgggcgg cagaggcgat
     1621 ccggcagcgc ctccagagca gccgccagcg cgaccatccc ggcggctacc agcacgatcg
     1681 agatcggccg cggcggatag agcgattccc aatccggcac gagcttccaa gccgcgatca
     1741 gcgccgccgc tgccaatagc gcccaccgca gtgcccacag gccgcggctg gtcggcgcga
     1801 gggcactcac cgcggctgcc gccacgccca cgtacggcag ccactgccaa tgccgctccg
     1861 gcttgagcgc cgcccagtcc ggcagcagca cgtagccgat gaatacggcc gccgcggctg
     1921 ccgaggccaa caggccgcgg tcgtcggtct tggcaggcag cgtccggcgc agcagccaga
     1981 ccgccgccca ggcgatcgcc gccggcgcaa ccaggccgac gagcaggttc atcgtccgcc
     2041 gctcgtgggc tgcgtcttct cgccgatggc caccttgagc gtcacctgct tcacgacctg
     2101 gcccggttcg tagttgatac cccagtcggt gcggtcaatg gtgaactcgc tggccagcgt
     2161 cagccccttg tcggtgacgg ccaccgtcgc cggaaagctg atctccttgg tcttgccgtg
     2221 cagagtcagg ttgcccgtga tcttggtctt gccgtccgcg gccggctcga tcttagtgga
     2281 ggcgaatttg gccgtcggaa actcgcgaac ctcgaggaag tcggcgctct tgagatggtt
     2341 ggtcagccgg ccgccgactt cagtccacag cgattcggtt tcgatatcga ccgagaccga
     2401 cttgagggcc ttgccgtcca cggccgcttg gcctttgaac ttggcaaagc cgccggtccg
     2461 cggatcgggc ttcttcggtg cggcgtgcgt gccgatgaag ttgatccgcg tgttgtcggg
     2521 cgaaagcgtc gccttgcctc ccgcgaccgg cacgggttcc gccgcagccg cccttaacgc
     2581 cagggccaga acgacaacca aagacaacgt ccgtttcatg cgcgaactcc gtaattgggg
     2641 ggattgtcag attctcaccg tcttgccgct cttgactgct tccgtctgct tgtggcagag
     2701 cacaatcgcg tcgcgggcca ggtcgccgcc gagcagggcc gacggtttct tgctcgcaat
     2761 cgaccgcgct acctcctgga tctcgtagtc gaacgccgcc agcatcgggt ccacggccgg
     2821 caatttcggc tgctccgcgt ggcctttgct gtcgagcagc gtcaagggcg tgatctgcag
     2881 cgtgccgccc gcgaggaccg cgagatcgaa atacagtgtc gccttttcga ggtgaatttc
     2941 aaacccgtgg gtgaacgagc ggccttgctg gttgacgacg ccgctggccg aggaaacgac
     3001 cagcgacttg ttcttgaact ggaattggct gacgcagtat tcgaccacac ctcctcgcag
     3061 gcggccctgg ctcgtcacgg cctgcggcat gccgaatacg agacggatga agtgggcgtc
     3121 gtggacgtgc aaatcgacga gcgggccgcc gactttgtcc gggttgaaaa agtccttgag
     3181 ccacgagggg tccgagatca cccgcttgaa atggccgccg agcaatttgc catacttgcc
     3241 gctctcgatt gccttccagg cgaacgcata ctccggcaga aacggcagca cgtgtcccac
     3301 cagcacttgc cgtttggcct tctttgcggc ggcgaccatc ttgtcgcatt cggccgccgt
     3361 cagccccatc ggcttttcga cgaacacatg cttgccggct ttgagggccg caatggtggc
     3421 ttcggcgtgc aagtggggcg gcagacagag atcgacgagg tcgatcgact tatcggccaa
     3481 gagcgcgggc agcgcggagt acttggccac ctccgacaga tcgaccgcct cgcccggcgg
     3541 gccgaaattg ccctggatgc cgcgccagtc gccggacagt cgcgtgcgat cgcgcgtgca
     3601 gaccgccgcg agcttcacgc ccttggcctt ctgataggcc aggtaatgaa tccaacccat
     3661 gaaaccgacg ccgaccaatc cgacgcgcag catgaggaaa ggctagggct aggtgctagg
     3721 gctagggacg caagcaacgc ggtacatcgt aatcagactt ccgaccctag ccctagcccc
     3781 cagccctagc cctttgccaa aaccagttgc tcgaaccgcg aataggcctc gtcccagtcg
     3841 gcggagggct tgggccggta ctcttgcact tcgaagctcg tccggatgat ctcgcgggac
     3901 tgggcgatat ccttgatcgc gccgcaggca acggcttgca ccatcaggtt gccgatggcg
     3961 gtggcctcga cgggtccggc gacgacgcgg cggccggtcg cgtcagcggc catctggcag
     4021 agcaggcggt tctgcgcccc gccgcccacg atgtggatcg tctcgatccg cccgccgatc
     4081 agctcttcca aaaagcccag caccatgcgg tagcgaaggg cgaggctctc cagtgcacac
     4141 cgaatgatcg cgccgtcggt gaccggcgcg gattgtccgg tcttagtgca aaagtcgaca
     4201 atggccgcgg gcatgttctc cggggcgacg aatgacgggt gatcgggatt gatcagcgcc
     4261 gccagcggcg ctcggtcgct ggcccgccgg ctcaactcct cccaggcgta gtcgcggccg
     4321 gcctctttcc aggcccgccg gcactcctga atcagccaca ggcccgcaat gttcttgagc
     4381 agccgcgtgg tgccgccgat gccgccttcg ttcgtgaaat tgagctgata gacgcggtcg
     4441 ttgatcaccg gctgcggcgt ctcgaccccc atcagcgacc acgtgccgct gctgatgtag
     4501 caccaatcgg gcacagtagg tcccgcctgc cgggcgggac ctgaggcccc ttccggcaga
     4561 aggggcctac tggccgccgg caccgccgcc acggcgctgg ccgtgtcgtg cgtgcccggc
     4621 aagatcacct cggcggtggt caaattcgtc tcggccgcca cttccggtcg gagcttgccc
     4681 agcttcgtcc ccggctgcac gatcgggccc aggatgtccg tccgaatgcc gaacttcgcg
     4741 agcagctccg ttgcccactt gcccgtcttg gggttgagga attgcgtggt tgtggcgttg
     4801 gtatattcgt tggccttgac gccggtcagc aaccagtgaa acaggtccgg gatcatcagc
     4861 cacgaatggg ccatttgaag cagcggcgaa ttcgcttgct tcatcgccag cagctggtag
     4921 agcgtattga actccatgaa ctgcaagccg gtcgccgcaa aaatctcctc gcgcgggacg
     4981 atcgcaaacg ccttgtcgag aatgccgacc gtgcggcggt cgcgatagtg gcacggattg
     5041 ctcagcagtt cgtcgctctt gctcagcagg gcaaaatcga cgccccacgt atcgacgccc
     5101 acgctcgcca cgtggctgcc atagaccttc gccgcggcct tgagcccgcg gagcgtctgc
     5161 tgccacaggt tcaggacatc ccagtgcatc cgccccgccg cctgcacgcc gccgttgtcg
     5221 aagcgataga cctcctccag cgccagccgc gtgccatcga acagcccggc cacgacgcgc
     5281 ccgctggaag cgccaagatc gacggcgaga tagacctgag aagcgtctgg catatcaatc
     5341 gtagagtaag cgtcctcgct tgctcagatc gaacaagcgt gctcattcta cgcgattcaa
     5401 tatccaccgc gtggggcgac atttgcccct atgccacgtc ttcaggcggc tccacgtcga
     5461 gaacggcagc cagggcggcc attaccgtgt cgcggttgag cgcgtgtctg gcgggaatta
     5521 ggtaacgcag gtgtgctcga caagggctgg catacgtcgg aatggtgaac tgccaggtcg
     5581 gggcggattg atcgcgcttt tggaacttga aggtccctcg ttcgcgcgtg ccaatccggt
     5641 agcttccgca aagctccgcg ttgtcctcgg caatcggcgt gtaaaaagtg acgtcccacc
     5701 ttcgctcgct gatgagccat tcctgctcgg cctcggagat cggctccagc gcactcgggc
     5761 cgacgacgag tagatcgatg ccgcggactt cgatgatgtc gtcgagtgcc ggcaagtaga
     5821 cccagtacga gcaccgacgc gtgctgcaga tgccgagcat cgtgccgtag tagccgatcg
     5881 tcgcgccgct ggcggcattg ttgggaaaca cggctacgcg gtaacgcggc acgaccgttc
     5941 cgttgaataa tgctgggcga ggcaggagcc tcgccgcgtg cgttcccagg caggagcctg
     6001 ggaacgagaa caatacctgg accccatcgc aggttagcgg ttggccaggc taagggccaa
     6061 ttcgcggcgc tggcggcgct cggacagctc ggtgctgcgt cgctgttgcc aattcgcttt
     6121 gcgccgggcc t
//
LOCUS       DASRJB010000059         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2276818, whole genome shotgun sequence.
ACCESSION   DASRJB010000059 DASRJB010000000
VERSION     DASRJB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276818"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..>2079)
                     /locus_tag="VFB80_01650"
     CDS             complement(86..>2079)
                     /locus_tag="VFB80_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN32484.1"
                     /translation="IYEEYHLRRTAGQAVVSRDYYERFPKSAAALRRLMGTDQLTNTT
                     QLVAAKQIKTYSAGQRLDDFDLITEVGKGAFASVFLARQVSMQRMVAVKVSADKGSEP
                     QTLGQLDHPNIIRVFDQRRLPDQHVKLMYMQFAPGGTLADVVKEVRGTKPEARSGTIL
                     AKAVTKAIEKSGAVVGDEAAWKRRLAGASWPETVCRLGIQLAYALDYAHHQGILHRDV
                     KPANVLLSADGLPKLADFNISFCSQLDGASPAAYFGGSVAYMSPEQLEACNPNHDRKP
                     EDLDGRSDMYALAVLLWELLHGERPFLDEEGAGGWLGMLDAMTQRRRALFPAGDVQPV
                     APKGPRDPITIRLEKALLKTLSPDPANRPADGAAMAREMTLCLNPRAWDLVNDLRSGW
                     RDWARRHPRWALFFVNLPPFFLAGALNLWYNQEYLVGKFVDPRMVTAFWWMVPFVNGL
                     LYPLGIYLILQMAEPMVRGLARLEAGDTQAEPLRLARHRAIVLGHRIAIVGMSLWLIA
                     GISFPIGIHLISGRFELDVYFKFILSMFACGIISCCLPFLATTWLTIRVFFPLLLANS
                     TPEPNEQKKLMTLSWYAGIYFAVSPVAPLIAVLLILMSSTLVPRLAIAGLIGTSILGF
                     FAAYLVWQRIRGDLAALAVVTRPPDQIGTTSQTTDSFLA"
BASE COUNT          396 a          652 c          688 g          343 t
ORIGIN      
        1 tggccggcga cctgcacgtc tatcgcaggc gaatgcgcgg gaagcaaaag aacgcggata
       61 cgctcgtgtg agacgatgac tggcgctacg ccaaaaatga gtccgtcgtc tggctcgtgg
      121 tgccgatctg atccggcggg cgggtgacga cggccagtgc cgccaggtcg ccccgaatcc
      181 gctgccagac caggtaggcg gcgaagaagc caaggatgct agtgccgatc agtccagcga
      241 tggccagcct gggaacaagt gtgctgctca tcagaatgag cagcacggca attaggggcg
      301 cgaccggtga cacggcgaaa tagatgcccg cgtaccaact gagcgtcatc agcttcttct
      361 gctcgttcgg ctcgggcgtg ctgttggcca agagcaatgg aaaaaagacg cggatcgtca
      421 gccaggtcgt cgccagaaac ggcagacagc acgagatgat tccgcaagca aacatggaaa
      481 ggatgaactt gaaatacaca tccagctcga agcgccctga gatcagatgg atgccgatgg
      541 gaaaggaaat gcccgcaatc aaccacagcg acattcccac gatggcgatg cggtggccga
      601 gcacgatggc ccgatgccgg gccaatcgca gcggttccgc ttgcgtgtca cccgcctcca
      661 gtcgcgccag gccgcgaacc atcggctcgg ccatctgcaa gatgaggtaa atccccagtg
      721 gatagagcag cccgttcacg aacggcacca tccaccaaaa ggcggtcacc atccgcggat
      781 cgacgaattt gccaacgagg tattcttggt tgtaccaaag gttcaatgcg ccggccaaaa
      841 agaatggtgg caggttgacg aagaacaacg cccagcgcgg atgccggcgg gcccagtcgc
      901 gccagccgct gcgcaggtcg ttgaccaggt cccaagcccg cgggttgagg cagagcgtca
      961 tctcgcgggc catcgccgca ccgtcggcag gccgattggc cggatcgggc gacagcgtct
     1021 tcagcagcgc cttctccagt cgtatcgtga tcggatcgcg ggggcctttc ggcgcgacgg
     1081 gctgcacatc gccggccgga aatagcgcgc ggcggcgctg ggtcatggcg tcgagcatgc
     1141 ccagccagcc gcccgcgccc tcttcatcga ggaacggccg ttccccgtgc agcagctccc
     1201 acagcaggac cgccagagca tacatgtcgc tgcggccgtc gaggtcttcc ggcttgcggt
     1261 cgtgattggg gttgcaggcc tcgagctgct cgggcgacat ataggccacg ctgccgccga
     1321 aataggccgc gggactcgct ccgtcgagct gcgagcagaa gctgatgttg aagtcggcca
     1381 gcttcggcaa gccgtcggcc gagagcagca cgttcgcggg cttcacgtcg cgatgcagga
     1441 tgccctggtg gtgggcgtag tcgagggcgt aggccagctg aatacccagg cggcagaccg
     1501 tctcgggcca gctcgcgccg gccagccgcc gcttccaggc ggcttcgtcg ccgacgaccg
     1561 cgcccgactt ttcaatcgcc ttcgtcaccg ccttggccag gattgtgccg ctgcgggcct
     1621 cgggcttcgt gccgcggact tccttgacga cgtcggccag cgtgccgccc ggcgcgaact
     1681 gcatgtacat cagcttgacg tgctgatccg gcagccgccg ctgatcgaac acgcggatga
     1741 tgttcgggtg atcgagctgg cccagcgtct gcggctcgct gcccttatcg gccgagacct
     1801 tgacggccac catccgctgc atcgatacct gccgggccag gaagacgctg gcaaaggctc
     1861 ccttgccgac ttcggtaatc aaatcgaagt cgtccagccg ctggccggcc gaatacgtct
     1921 tgatctgctt ggcggcgacg agttgcgtcg tgttggtgag ctggtcggtg cccatcagcc
     1981 ggcggagggc ggcggccgac ttggggaagc gctcgtaata gtcgcgggag acgacggcct
     2041 gcccggccgt gcggcggagg tgatactcct cgtagatca
//
LOCUS       DASRJB010000060         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3406797, whole genome shotgun sequence.
ACCESSION   DASRJB010000060 DASRJB010000000
VERSION     DASRJB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406797"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFB80_01655"
     CDS             <1..332
                     /locus_tag="VFB80_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32485.1"
                     /translation="GHRPASYSSGGGGSSNMYATIRSYDCQVDLPAKLRGQFMANYRE
                     HIRATLAKLADRVGGAGSISDGRGLKGFDYEYGKGRVHGTVVVRSTSGDDGLSLFIFV
                     HEYDSRR"
     gene            complement(444..4001)
                     /locus_tag="VFB80_01660"
     CDS             complement(444..4001)
                     /locus_tag="VFB80_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32486.1"
                     /translation="MKARTPVAALAACLAALVLSPLRAADWPQFMRSAEHTGDAADEE
                     LRLPLGLAAQVKLNDAVLTSPAIVAGRAYVVDQMGTAYCIDPAAQKIVWQASPDGVAA
                     MGSNTSSPCVAGGRVFFGTTAGRLHILNSEDGSVYKTLAIGSPILSAPTLANDSIYSQ
                     ALDAVLRCIDLDGNERWQWDHYQRYQEPPEITKSQERERGHPGSYDRPHYGGGDVAVS
                     GRKVVTSFGWDIVCLEDAGREARIVWCNRAPSGRDGSSPMSSSISGDWIYNAGMGADG
                     HLALTRIALADGKVAKNEGGRREAYPWITPAVRGAAAVLRDSSYGKNGIQLFDFDSRR
                     AIASWRDEKDSTPIASSHVLTKNHLVATTLRGEVLAIELNGKPGTDAFRYLMTNAKGI
                     ASTPAVVGGKIYFGSDDGYFYMLAADADTPGPPDALVAAEPQPPPAGRHWPSTYGNAG
                     NTSFQDDPAIAPPLRVRWASRGFGHQLAPCVASGGDLFTVTLTGLITCQEQATGRLRW
                     RVQMPGPEWSTSSGMLAEGGRLFVPRPTFGRMDGEFYCLDQRTGRLLWSARIGGRYIW
                     ERAAPVAAAGMVAFGFGTPAPGDKPDGDTSPTGTLIQAWDQATGQPAWQVELNVAGTR
                     SGSIAGCTDGETMYFTAGAEAWNWRQEGSKQRGETVAIEARTGKVLWRTHELFGASYP
                     VLAQGKLLLSADGLSCVSAADGKLVWNRPAPGYTRFSVGDDYIVMRGYGGHGEKIFLA
                     DGKEDLTCRELGGATHACSSVALTSRSRSAASMSATCGPANCCGNRPVSRRGAASTPS
                     WPTAASSGPAPPAAWSIAGSRSARNEHSRPGGLRVPVEIDPGPRRVGGVVLDERIHAK
                     RPDRHGGHVPDLVVGDARRGKDRRLTGLLLPLISRHLIDIPDAGLAEDQEVATDRIGG
                     DYGLHRAYADRGSFQRGDFARSVELIQVARAVAAAQQHDFVDRAVLGEVVIDAHRQVL
                     GQRGPLLALLAERKDVSLAAAAAGEDQRLQLREPGQPDAAGQVLVRVDRRQRGPFLRL
                     DVVAVNEAPLAAERPVVEDAPLGVEGVVVVAVRRRLAEPLRLRQSSPGVAVGGIGDEP
                     RRIVVVAAQIQNLPRRRIPHRSFVVAKDRAAVALSLERGHDVLRLQLGPLASLQRVGQ
                     FAQLGQLTPGGQGQTEAEGKDAGSRFHRIAPFRENSGVR"
     gene            complement(4015..>4345)
                     /locus_tag="VFB80_01665"
     CDS             complement(4015..>4345)
                     /locus_tag="VFB80_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32487.1"
                     /translation="NTWQNKQPPVQPAGVHGAGFVYEPFQKVLLLQSGKRETQYGGGS
                     DSITWTYDANTNTWTDLNVPSGPGNPWVGAMDFDPEHNVLVLFNHRDRQVWAYRYKQV
                     AVGTRAE"
BASE COUNT          708 a         1470 c         1434 g          733 t
ORIGIN      
        1 tcgggcatcg cccggcgtcg tactcgtcgg gcggcggtgg ctcgtcgaac atgtacgcca
       61 cgatccgttc gtacgattgc caggtcgatc tgccggcgaa gctgcggggc caattcatgg
      121 ccaactaccg cgaacacatc cgcgccacgc tcgccaagct ggccgatcgc gtcgggggag
      181 ccggctcgat aagcgacggc cgcggcctga aggggtttga ttacgagtac ggtaaaggcc
      241 gcgtccacgg caccgtcgtt gtccgctcga ccagcggcga cgatggcctg tcgctgttca
      301 tcttcgtcca cgaatacgac agccggcgat aacaacccga agcgtaagcg cgtaagcgag
      361 gggttttcta agttcgggcc gcttcgacaa agtccctcgc tcacgcttcg ggtttgtgtt
      421 aggagctcta aagcggggac gagtcaccgc acgccagagt tttccctaaa gggggcaatt
      481 cgatgaaaac ggctgccggc atctttgcct tctgcctcgg tctggccctg cccgcctggg
      541 gtcaattgcc cgagttgggc aaactggccc actcgctgca ggctggccag tgggccgagc
      601 tgaagacgga gaacatcgtg cccacgctcg aggctcaggg caacagcggc gcgatctttg
      661 gctacaacga aggagcggtg tgggatccgg cgtcgcggca gattctgtat ctgggcggcg
      721 accacaacga tccggcgcgg ttcgtcgcct atgccgccga cggcaacgcc tggcgaactc
      781 tgccgcagcc ggagtggctc ggcaagacgg cgacgcacgg ctacgaccac aacgccctcg
      841 accccgagcg gggcgtcttc tactaccggc cgttcagcag caaggggggc ctcgttcacc
      901 gctacgacat caaggcggag aaatggacct cgctgccgcc gatcgacacg gacgagtact
      961 tggcctgctg cgtcgggctg gcctggttcc cggagctgaa gtcgctggtc ttcgccagcg
     1021 gcggcggcgg caagggagac gtctttgcgt tcagcgagga gagccagaag tggtccacgc
     1081 tggccaagga cctgccgatg ggcgtctatc acaacttcgc cgagtacagc ccggtccaca
     1141 aagtcgtgct gctgggcggc ggcaacggct cgcgcgactt gtataagctc gacgctgcgg
     1201 gcaaagtcac cacgctgaaa gctgccccga tcggcatagg cacgatgcag accatagtca
     1261 ccgccgatcc ggtcagtggc gacttcctgg tcttcggcaa gaccggcgtc tggtatgtct
     1321 atgaggtgac gcgagatgag tggaaggagc agcccggtca ggcgccgatc tttgccccga
     1381 cgcgcgtcgc cgacaacaag gtctggcacg tgaccgccgt gccgatcggg acgtttggcg
     1441 tgaatgcgtt catcaagtac tacgccaccg acacgccgcg ggcctgggtc tatctctaca
     1501 ggcactcgca gcccgccagg ccgtgaatgc tcatttcggg ccgaccggct cccagcaata
     1561 gaccacgccg ctggcggcgc tgggccagaa gacgcggccg tcggccagga cggcgttgac
     1621 gcagccccgc ggcgcgaaac cgggcgattg ccacagcaat tcgccggtcc gcacgtcgcg
     1681 gacattgagg ccgccgaccg tgatcgcgaa gtcagcgcta ccgagctgca agcgtgcgtc
     1741 gccccgccca gctcacggca cgtgagatct tccttgccgt cggcgaggaa gatcttctcg
     1801 ccgtgtccgc catagccccg catcacgatg tagtcgtcgc cgacgctgaa ccgcgtgtag
     1861 ccgggcgcag gccgattcca gaccagcttg ccgtcggccg cgctgacgca agagaggccg
     1921 tcggcgctga gcagcagctt gccctgggcc agcacgggat acgaagcgcc gaacagctcg
     1981 tgcgtccgcc acagcacctt gccggtgcgg gcctcgatgg ccaccgtttc cccgcgctgc
     2041 ttgctccctt cctgccgcca gttccaggct tcggcgccgg cggtgaaata catcgtttcg
     2101 ccatccgtgc agccggcgat cgagcccgac cgtgtgccgg cgacgttgag ctcgacctgc
     2161 cacgccggct gaccggtggc ctggtcccag gcctgaatca gcgtgccggt cgggctcgtg
     2221 tcgccgtcgg gtttgtcgcc gggcgcgggc gtgccgaagc cgaaggcgac catgccggcg
     2281 gcggcgaccg gcgcggcccg ctcccagatg tagcgcccgc cgatcctcgc cgaccagagc
     2341 agccggccgg tccgttgatc gaggcaatag aattcgccgt ccatgcggcc gaaggtgggc
     2401 cgcggcacga acagccggcc cccttcggcc agcatgcccg atgacgtgga ccattccggc
     2461 cccggcatct gcacccgcca ccgcaggcgt ccggtcgcct gctcctggca ggtaatcagc
     2521 cccgtcagcg tgacggtgaa caggtcgccg ccgctggcca cgcagggcgc gagctgatgg
     2581 ccgaagccgc ggctcgccca ccgcacgcgc agcggcggcg cgatcgccgg atcgtcctgg
     2641 aacgacgtgt tgccggcgtt gccataagtc gaaggccagt gccttcccgc gggcgggggc
     2701 tgcggctccg cggcgaccag cgcgtcgggc gggcccggcg tatcggcgtc cgcggccagc
     2761 atatagaagt agccgtcgtc ggaaccaaaa tagatcttgc cgcccacgac cgctggcgtg
     2821 gaggcaatcc ccttcgcgtt ggtcatgagg tatcgaaatg cgtcggtgcc tggcttgccg
     2881 ttgagctcga tcgccagcac ctcgccgcgg agcgtcgttg ccaccaggtg gttcttcgtc
     2941 aggacgtgcg agctggcgat tggcgtggag tccttctcgt ctcgccagga agcgatcgcg
     3001 cggcgggaat cgaagtcgaa cagctggatg ccgttctttc cgtacgacga gtcgcgcagc
     3061 acggccgccg ctccgcgcac ggccggcgtg atccagggat aggcctcgcg gcggccgcct
     3121 tcgttcttcg ccacctttcc gtcggccagg gcgatccgcg tgagggccag gtgcccgtcg
     3181 gcgcccatgc cggcgttgta gatccagtcg cccgagatgg aggacgacat gggagatgag
     3241 ccgtcgcggc cgctcggagc gcggttgcac cacacgatgc gcgcctcgcg gccggcatcc
     3301 tccaggcaaa cgatgtccca gccaaagctc gtcacgacct tccggccaga cacggccacg
     3361 tcgccgccgc cgtagtgcgg ccggtcgtag ctgcccggat ggccccgctc gcgctcctgg
     3421 ctcttcgtga tttccggcgg ctcctggtac ctctggtagt ggtcccactg ccaccgctcg
     3481 ttgccgtcca ggtcgatgca gcgcagtacg gcgtccagcg cttgagaata gatcgagtcg
     3541 ttggccagcg tcggcgcgct caagatcggc gagccaatcg ccagcgtctt gtataccgat
     3601 ccatcctcgc tattcaggat gtgcagccgg ccagccgtcg tgccgaagaa gacgcggccg
     3661 ccggcgacgc agggcgagct ggtattcgag cccatcgccg ccacgccgtc gggcgaagct
     3721 tgccagacga tcttctgagc cgccggatcg atgcagtagg ccgtgcccat ctgatcgacg
     3781 acgtaggccc gcccggccac gatcgccggc gaagtcagca ccgcgtcgtt cagcttcacc
     3841 tgggcggcga ggcccagcgg caggcgcagc tcttcatcgg cggcatcgcc cgtatgctcg
     3901 gcgctgcgca tgaactgtgg ccagtcggcc gctcgcagtg gcgagagtac gagcgccgcc
     3961 aggcatgcag ccagcgctgc gacgggtgtt cgagctttca tctggaattg ctcctcattc
     4021 agcacgcgtg cccacggcga cctgtttgta gcgataggcc cacacttgcc ggtcgcggtg
     4081 gttgaacagc accaagacgt tgtgctcggg atcgaaatcc atcgccccga cccatggatt
     4141 accggggccg ctcggcacgt tcaggtcagt ccaggtattc gtgttggcgt cgtaggtcca
     4201 ggtgatcgaa tcgctgccgc cgccgtattg cgtctcgcgc ttgccgcttt gcagcagcag
     4261 caccttctga aacggctcat agacaaaccc cgcgccgtga actcccgccg gctgcacggg
     4321 cggctgcttg ttctgccacg tatta
//
LOCUS       DASRJB010000061        15222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3970713, whole genome shotgun sequence.
ACCESSION   DASRJB010000061 DASRJB010000000
VERSION     DASRJB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15222
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3970713"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..285
                     /locus_tag="VFB80_01670"
     CDS             67..285
                     /locus_tag="VFB80_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32488.1"
                     /translation="MLALLMANSALPAQDALTPAEFQKLHDELTPEAAAWEAVPWRLS
                     VLEACAVAAKEKKPVYMLVRSGHPLGCV"
     gene            394..1188
                     /locus_tag="VFB80_01675"
     CDS             394..1188
                     /locus_tag="VFB80_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32489.1"
                     /translation="MQDAEGQIFAKVLRQAGLGLEGGSQGVFCFTADGKLLSHANVAE
                     AEPERRLLTGVLRKFDPAAALAAEVLTPVAGDKPPLPQPPPGALIVDVTSKVLGGYPE
                     TKDRRETFAQKSLGRDHLWIRADEAAALAEGKFPDSLARRLARYHLVDNTRGEPPMWE
                     ANEVKRLDLKIADGRISGTVHLETRRGDRGYTADIAGVIETSENKVTRLDLVAKGQFW
                     GEGPFTWGAPPGKFPFAVACRLATEDCAATRVPPQAARLGSRDYLE"
     gene            1298..2224
                     /locus_tag="VFB80_01680"
     CDS             1298..2224
                     /locus_tag="VFB80_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32490.1"
                     /translation="MNRRGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMQCQNH
                     LKQLALGLHNYHDTFNRLPPGQWNNFYSNDVPWIRGCWVQPLLPYIEQKNLYEIYEAS
                     LRTNGNWALLCPNKDTLIRTLICPSDGNSPKIQTRDTNTVNGVAGTMQGLHTNYVVCS
                     GSTAYGTGGLSLNGMFYTKSFLRLAEVTDGLSNTLMLSEICVVRDTTANDLRGRYCNS
                     WEGNSWFTTLNPPNTSVRDTQNYQGVSIKQAPQTTVANNGTQALYARSYHASGVNASL
                     GDGSVRFVSNSIHAAVYQSLGTRNLGETTGDF"
     gene            2244..2633
                     /locus_tag="VFB80_01685"
     CDS             2244..2633
                     /locus_tag="VFB80_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32491.1"
                     /translation="MLHKTLRAGIIFIALAAASCAPENPLLTIGGSVTLDGAPLPDGD
                     IVFTPTDPQFGSEGGKIKDGVYQAAVHKGVSKVQIRATRPVPGKKGPMGEQLIEDYIP
                     ARYNDASTLTIDASASQLKHDFALKSK"
     gene            complement(2650..3432)
                     /locus_tag="VFB80_01690"
     CDS             complement(2650..3432)
                     /locus_tag="VFB80_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZN32492.1"
                     /translation="MRPYILSEVNYAYTKANPYEVAVLPLGATEPHNLHLPYGTDMYE
                     GTIIGEKICEEAHKRGAKVLLLPTIAYGTETNMSRFPLAINVDPTTLYRFVTDIVKSC
                     INSGVRKIVLLNSHGGNEMKPLLRELADKIDGQLFLCNWYNVLDHEAVGKIFEHADDH
                     AGEMETSFILAFYPHLVAKTKDGKLAADDGQTAKTRFEAVNRGWVSITRPWHLLTTNS
                     GSGYPHSASADKTHAMLKILVDRLATFLTELSAAKMDERFPY"
     gene            complement(3460..5295)
                     /locus_tag="VFB80_01695"
     CDS             complement(3460..5295)
                     /locus_tag="VFB80_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32493.1"
                     /translation="MGRKLQEPKNRRTEIWFFGSLVLWFFSLPAAADELQALAAKCNQ
                     LGLAEQAAITQKWNIPRHPGRQYLFIPPASDPAAPQSGAAMVVRQWYDKFLTIRRERA
                     AALYAGARQASDERQPARAYQLLHETLREDPDHAEARRVLGYVKDARGQWTTPEWEKL
                     VVETAKTNHPQTGWRAGSYQRLDTPHFHIATNHTKSEALEAGRELENLHTLWRQIFFR
                     YWSTPEALAARLAGGNQPLAPPRPAKMQVVLFKNRQEYTAKLSQVERLAAATLGYYSS
                     ESKVAYFFAGDTSVYPTWYHEAAHQLFQESVPGAVALPGADRNFWALEGAALYMESLA
                     RHDGYWTAGGCEADRLQLARYRALSGDFLVPMQRLSAMGRAALQQDADLRKLYSQSAG
                     LAHFLIDGAEGRHREAFVDLLAVIYRGEDLPDYLAKATAEPLAALDEQYLKFLNVTDA
                     DLAGIPDPSRLKNLSLGRTAITDAGLANMAGCKELLFLDLSATAASDAGLAHFAAASK
                     LDQLFLEGSKVTAASLPLIGGFKQLTQLDLARLPLTDESLAPLAGLKNLQRLYLHGTP
                     LSDASLVHLRGLKQLETLDTAGTKITPEGRKRLQIVLPKLKLASP"
     gene            complement(5299..6543)
                     /locus_tag="VFB80_01700"
     CDS             complement(5299..6543)
                     /locus_tag="VFB80_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32494.1"
                     /translation="MSYRLAILGIALAALPLAAQDNWPQFRGPQGHGTSKSTGLPTTW
                     SEKEHVKWKTPLHDRGWSSPVIWGDQIWVTAATEEGKTDFAICIDKNTGKIVHDLKLW
                     DNEKVYPLGNALNSYASCTPAIEEGRVYVHFGSYGTACLDTASGKTLWQRRDLPCEHF
                     RGPGSSPILFENLLIFHMDGYDFHYIVALDKQTGNTVWKMDRNIDYGTTNGDLMKSFC
                     TPLVIEAAGKTQLISPSSKACMSYDPRTGREFWRVRYASHSGAAMPLFGHGLVYVNTG
                     FGAADLLAVKPDGEGDVTTTHVVWASKKSIGSKPSQVLVGDLIFNVDDNPGVATCIDA
                     KTGEELWSKRLSGEFSASPLYADGKIYYCGQDGITTVVKADREYTELAKNQFADGFMA
                     SPAVTGKALILRTRSAIYRVEE"
     gene            complement(6617..8083)
                     /gene="lpdA"
                     /locus_tag="VFB80_01705"
     CDS             complement(6617..8083)
                     /gene="lpdA"
                     /locus_tag="VFB80_01705"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZN32495.1"
                     /translation="MHSRLVVLGGGPGGYAAAFLAADEGLEVTLVEADSRLGGTCLLR
                     GCIPSKALLHVARVISEVEELRHDWGIEYPRPKLDVNKVRERKEKVILALSGGLAQIA
                     KRRKVRVIRARGVFENGTTLRLDGDDASIPSDRKLTFDHCIVATGSTPAMPPAFNIGS
                     DRVMDSTGALALAEIPQTLLVIGGGYIGLEMGTVYANLGSQVSVVELLDGLLPGADRD
                     LVKPLHKRLNTLFGERIFLNTKVGSLGLRDNKVEVAFEGPGKFGTEQYDRVLVSVGRR
                     PNSRGFGLENTQVEIDAKGFVKVDRSMRSADPHILAIGDVAGEPMLAHKATHEGRVAA
                     EVVAGKPVAFDRLAIPAVVFTDPEIAWCGLTEEQAKQENRPYETGVYPWAASGRAIAL
                     GRTEGLTKILIEPETERVLGVGIVGPGAGELIAEAVLAIEMGCEVTDITESIHAHPTL
                     SETLMNAGEAFFGTATEIYKPKRSPPPETQGKPAEGAA"
     gene            complement(8193..8807)
                     /gene="rpsD"
                     /locus_tag="VFB80_01710"
     CDS             complement(8193..8807)
                     /gene="rpsD"
                     /locus_tag="VFB80_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZN32496.1"
                     /translation="MARYNGPKARINRRLKTLVYETAGAARALERRDNPPGMHVKGGR
                     KMSNWGLALFEKQKIKHHYGLMEKQLRRYYRMVVAKKGDTGAQMLALCERRLDNVVRR
                     GGLTKTRPQARQGIVHGHFYVNGIKVTSPSYQLRPGDVITIRPKEQIYGLYKGVVGDG
                     NNVQTPDWITTDPTSLRITMVGLPGPGDFSLPVDVNIVVEFLAR"
     gene            9000..10889
                     /locus_tag="VFB80_01715"
     CDS             9000..10889
                     /locus_tag="VFB80_01715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32497.1"
                     /translation="MPLERLGPYKLEKVLGRGGMGAVYVGLNDETGERAAVKVLSGHL
                     ADDTNFRERFKQEIETLKRLLHPNIVQLFGYGEEDGHLFYVMELVSGRSLQEEIAAGR
                     RFSWREVARIGVGVAQALKHAHDRGIIHRDLKPANLLMDASDHVKLTDFGIAKLYGGT
                     NVTAEGGVLGTADYMAPEQAEGKQPTSRCDLYALGSVMYALLTGRPPFVGRTIYEVMS
                     ALQKEPPTPVRRLAPDTPAEFENIILQLLHKDPHQRIPTALALANRLKAMEHALSLET
                     RVLDPLDDQPEVAEPLPDEALPSSAKTASANVNANPTLPIELAQSDEYRLASDAPTLA
                     TDARGGQSNLTRLGTQADKRTGAGESGRKTDSDQPAAPSNTAAAALARFTTVSEAELR
                     GARETDETPVRQWLLVGGLLALAAVLIAGVALLATRPPSADRLFASVQAAADSGPDEL
                     VLHESDLVQFLQHHSSDPRAAEVRAWQDDMEQYQLQKQLERRARRPSGAEGLTPAERA
                     YLAALRMESSDPEAALARYEAIASVYGGPPDPALNPIQQRISQQCIALARQAAERLRP
                     TAAKLNAEEKQAVRRQLERADKLATTDRAAAEKVWRGIDTLYASQPWASDLVAEARAK
                     LAESP"
     gene            11050..12099
                     /locus_tag="VFB80_01720"
     CDS             11050..12099
                     /locus_tag="VFB80_01720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN32498.1"
                     /translation="MKRRALLVVAVLGLLALGWLAESHFAAGQNEAAATIKGWKAGVG
                     WGWIWGKDDEVGALNAMTDQSRAAALKLAATGKVYDLGIAYSRRSYKWPGHSPGEIMS
                     FRSPAGEEAQKESVVNPMGNSSVTRWHSNALFMNDNVATQIDGLGHITISADHHWYNG
                     FKEAEHGGDFGIQKCDNVSIPPIVARGVLLDIAAARKVDELPTGYPISPADVDAALAQ
                     QKVSLRPGDVVLVRTGTLRHWGTDGDNHAKIGQHDSAGITLQTAKYLVERHGAMLIAS
                     DTSGLEVNPAPAGSNSFIPVHKYLLVEQGVHIGEFHYLEDLARDKVYEFCYVATVNKI
                     QGAVAGFTLRPLALR"
     gene            complement(12207..13496)
                     /locus_tag="VFB80_01725"
     CDS             complement(12207..13496)
                     /locus_tag="VFB80_01725"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN32499.1"
                     /translation="MIAVSEPVPPDASVFAAFVGLDWADQKHDLVLYDPRGGKPEHSQ
                     LPHTPEAIDEWACSLCERFGGRPVAVCFEQTRGAIAYALLKYDFLVLFPLPPARLKSY
                     RESFSTSGAKDDPTDAALLADYLLRHRDQLRVWRPDSPATRELLWLAEARRDAVNQRT
                     RLSNQLTAVLKRYYPQALVLVGEDVDTPLACDFLSKWPTLTAVQAALPATLRKFYYAH
                     SCRSEERIAERLELARSAVPLTTDAALLGAGTLQVQCLVAQLRALHKAIAKYEARLAE
                     LMEAHPDAALFRALPGAGPALAPRLLAAFGTDRERFQSAAELQTLSGVAPVTERSGKR
                     STVRRRWACPKFLLQTFHEFAYCSSKRSVWARAFYLQQRAVGKGHHAALRTLAFKWIR
                     ILFRCWKTNTPYHEQRYLDALRRHSAPLLTFLPTASS"
     gene            14273..15139
                     /locus_tag="VFB80_01730"
     CDS             14273..15139
                     /locus_tag="VFB80_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32500.1"
                     /translation="METLPEFAGRLLPAVLFACLALYFLIRGATAGPGSRRLANLALF
                     CGLTGLVLPEISLGLSPAANSALFLASGAIRIFLGVAGITLAAAALWTRGDGGVGIAR
                     PVTAAGFSFLHAFIGAGLVLFSSFTWASTPWDFQSPDGAFRLRLPSQQWKQAPVDDRA
                     DAIAFVCSSPRMQARVVAVRQEQTAADFALAVEALRARLDSLPTRRDTAEDREGTTTS
                     GGSYRYVTFMDSTPDGNPVFVAYSVTWCPRQQTVIEVLVEGLPRLLSQTGKAGEMHRF
                     QKSAETICLSVE"
BASE COUNT         2639 a         4910 c         4956 g         2717 t
ORIGIN      
        1 gaaatgcgcc tttagcggct tccgattttc atgaatactt gccttcgcag catttcgacg
       61 cgcgccgtgc ttgcgctgct gatggcaaat tcagcgcttc cggcccagga cgccctgacg
      121 ccagccgagt ttcagaagct gcacgacgag ctgactccgg aagcggctgc gtgggaggcg
      181 gttccctggc ggctgtcggt gctcgaagcg tgcgcggtcg cggccaagga gaaaaagccc
      241 gtctatatgc tggtccgcag cggccacccc ttgggctgtg tctgaaacaa cggcatcgcc
      301 gaccgtgcgt cggctctgca agacgaggaa atcctgcgcc tgctgcaaac gcagttcatc
      361 cccgtcgccg tcgatcagca cattcatcgc cgcttgcagg acgccgaggg gcagatcttc
      421 gccaaggtgc tgaggcaagc cggcctgggc ctggaagggg gttcgcaagg cgtcttctgc
      481 ttcacggccg acggcaagtt gctgagccac gccaacgtgg ccgaggccga gcccgagcgc
      541 cggctgctga cgggcgtgct gcgcaagttc gatcccgccg ccgccttggc cgcggaggtg
      601 ttgacgcccg tagccggcga caaaccgccg ctgccacagc cgccgcccgg cgcgctgatc
      661 gtcgatgtca cgagcaaggt gctcggcggc tacccggaaa cgaaggaccg gcgggaaact
      721 ttcgcgcaga agtcgctcgg ccgcgatcac ctctggattc gcgccgacga agcggccgcg
      781 ctggccgagg gcaaattccc ggacagcctt gcgcggcggc tggcccgcta tcacctggtc
      841 gataacaccc gcggcgagcc gccgatgtgg gaagccaacg aagtgaaacg cctcgacctg
      901 aagatcgccg acggacggat cagcggcacc gtgcacctgg aaacgaggcg cggcgaccgc
      961 ggctacacgg cggacatcgc cggcgtcatc gaaacctccg agaacaaggt cacccggctg
     1021 gacctggtgg ccaaggggca gttctggggc gagggcccgt ttacatgggg cgcgccgccc
     1081 ggcaagttcc cgttcgcggt ggcctgccgc ctggccaccg aggactgcgc cgccacgcgc
     1141 gttccgcccc aggccgcccg gctcggttcg cgggactatc tcgagtagac ccgcgcgcgg
     1201 aaaccgcacc gttgttcttg ctcgccttct caaaccgact agactctatc gcgatcgtcc
     1261 ggtcggtcgc acaggagatc gtggtatgga gtcactcatg aatcgccgag gctttacgct
     1321 ggtcgagttg ctggtggtga ttgccattat cggcgtcctg gtagcgttgc tgctgcctgc
     1381 cgtgcaggcc gctcgcgagg ctgcccggcg gatgcagtgc cagaaccacc tgaagcagct
     1441 cgccttgggt ctgcacaatt accacgacac attcaaccgc ttgccgccgg gacagtggaa
     1501 caacttttac tccaatgacg tcccttggat tcgcggctgc tgggtccagc cgctgttgcc
     1561 ttacatcgag caaaagaacc tgtacgagat ctacgaagcc tcgctccgca ccaatggcaa
     1621 ctgggccctc ttgtgcccga acaaggacac cctgatccgc acgctgattt gcccctccga
     1681 tggcaacagc cccaagatcc agacgcgcga caccaacacg gtcaatggcg tcgccggcac
     1741 gatgcaaggg ctgcacacca actacgtcgt gtgctccggc tcgacggcct atggcacggg
     1801 cggcctgtcg ctcaacggca tgttctatac gaagtcgttt ttgcgactgg ccgaagtgac
     1861 cgacgggctt tcgaatacgc tgatgctcag cgagatctgc gtcgtgcgcg ataccacggc
     1921 caacgacctg cgcgggcggt attgcaactc gtgggaaggc aacagctggt ttacgacgct
     1981 caatccgccg aatacttccg tccgcgacac gcagaactat cagggcgttt ccatcaagca
     2041 ggcgccgcag acgacggtgg ccaacaacgg gacgcaggct ctgtatgccc gcagctacca
     2101 cgcctccgga gtcaatgcga gtctgggaga cggttcggtg cggttcgtca gcaattccat
     2161 ccatgcggcc gtctatcaat cgctgggcac gcgcaacctg ggcgagacta ccggggactt
     2221 ctagcctcct gcagcccgag aacatgctcc acaagactct tcgagccggc atcatcttca
     2281 tcgcactcgc ggcggcttcc tgcgcgcccg agaaccccct gctgacgatc ggcgggagcg
     2341 tcacgctgga cggcgctccg ctgcctgacg gcgacattgt gttcacgccc accgatccgc
     2401 agttcggctc cgagggcggc aaaatcaagg acggcgtcta tcaggccgcc gttcacaagg
     2461 gcgtcagcaa agtgcagatc cgcgccactc gccccgtccc cggcaagaag ggcccgatgg
     2521 gcgagcagtt gatcgaagac tatatcccag ccaggtacaa cgacgcttcc acgctcacca
     2581 tcgacgcctc cgccagccag ctgaagcacg acttcgcctt gaagtcaaag tgagggccag
     2641 ccggaatcgc tagtacggaa accgctcgtc catcttggcg gcggatagtt cggtgagaaa
     2701 cgtcgccagc cgatcgacaa ggatcttgag catggcgtgc gtcttgtcgg cgctggcgga
     2761 gtggggatag cccgagccgc tgttggtggt cagcaggtgc caggggcggg tgatgctgac
     2821 ccagccgcga ttgacggctt cgaaccgcgt cttggcggtc tggccgtcgt cggcggccag
     2881 cttgccgtct ttcgtcttgg cgaccaggtg cggatagaag gccagtatga acgacgtctc
     2941 catctcgccg gcgtggtcgt cggcgtgctc gaagattttc ccaactgcct cgtggtccag
     3001 cacgttgtac cagttgcaga gaaacagctg cccgtcgatc ttgtcggcga gttccctgag
     3061 cagcggcttc atctcgttgc cgccgtggct gttgagcagc acgattttcc gcacgccgct
     3121 gtttatgcag ctcttcacga tgtccgtcac gaagcggtag agcgtcgtcg gatcgacgtt
     3181 gatcgccagc gggaagcggc tcatgttcgt ctcggtgccg taggcgatcg tcggcagcag
     3241 caggaccttc gctccccgct tatgcgcctc ttcgcaaatc ttctcgccga tgatcgtccc
     3301 ctcgtacatg tccgtgccgt agggcaaatg gaggttgtgc ggctccgtcg cccccagcgg
     3361 cagcacggcg acttcgtacg gattggcctt cgtgtaggcg tagttgactt cggagaggat
     3421 gtagggacgc atgcggagag ccagggctgg ggagttgggc tagggcgacg ccagcttgag
     3481 ctttggcagc acgatttgca atcgcttacg gccttctggc gtgatcttgg tgccggcggt
     3541 gtcgagcgtc tcgagctgct tgaggccgcg gaggtggacg aggctggcat cgctcagggg
     3601 tgtgccgtga aggtagagcc tctgcaggtt cttcagtcct gccagcggcg cgagcgactc
     3661 gtcggtcagc ggcagccggg ccaggtcgag ctgcgtcagc tgcttgaagc cgccgatcag
     3721 cggcagcgat gccgcagtca ctttgctccc ttcgaggaag agctgatcga gcttgctcgc
     3781 ggcggcgaag tgcgcgaggc ccgcgtcgct ggcagccgtg gcggatagat cgaggaacag
     3841 cagctctttg cagccggcca tgttggccag tcctgcgtcg gtgattgccg ttcgcccgag
     3901 gctgaggttc tttagccgcg agggatcggg gatgccggcg aggtcggcgt cggtgacatt
     3961 gagaaacttg agatactgct cgtcgagcgc ggcgagcggc tcggccgtgg ccttggcgag
     4021 gtaatcgggc aaatcttcgc cccgatagat caccgccagc aaatcgacga aggcttcgcg
     4081 atgccggccc tctgcgccgt cgatcaaaaa gtgcgccagc cccgcggact ggctatagag
     4141 cttgcggaga tcggcgtctt gctgcagcgc cgcgcggccc atggcgctca gccgctgcat
     4201 cggcacgagg aagtcgcccg acagagcgcg gtagcgggcg agctgcaggc ggtcggcctc
     4261 gcagccgccg gcggtccaat agccgtcgtg gcgggcgagt gattccatgt agagcgccgc
     4321 cccttccagg gcccagaagt tgcgatccgc gcccggcaga gcgaccgcgc ccggcacgga
     4381 ttcttgaaag agctggtgcg ccgcctcgtg ataccaggtg ggatagaccg acgtgtcgcc
     4441 ggcaaagaaa taggcaacct tactttcgct cgagtagtag cccagcgtcg ccgcggccag
     4501 ccgctcgacc tggctgagct tcgcggtgta ttcctgccgg ttcttgaaca ggacgacctg
     4561 catcttggcc ggccgcggcg gcgcgagcgg ctgattgcct ccggccagcc gcgcggcgag
     4621 ggcctcgggc gtgctccagt agcgaaagaa gatctgccgc cagagcgtgt ggaggttttc
     4681 caattcgcgg ccggcctcca gggcctcgct cttggtgtgg ttcgtcgcga tgtggaagtg
     4741 cggcgtatcg agccgctggt agctgcccgc ccgccagccg gtctgcggat ggttggtctt
     4801 ggccgtttcg acaacgagct tctcccactc gggggtcgtc cactggccgc gggcgtcttt
     4861 gacgtagccc aacacgcggc gggcctcggc atgatcgggg tcttcgcgga gcgtctcgtg
     4921 cagcagctga tacgcgcggg ccggctgccg ctcatcgctg gcctgcctcg cgcctgcata
     4981 cagcgctgcg gcccgctcac gacgaatggt caggaacttg tcgtaccact ggcgaacaac
     5041 catcgccgcg ccggactggg gcgcggccgg atcgctcgcc ggcggaatga acaaatactg
     5101 ccgccccgga tgccgcggga tgttccactt ctgcgtaatc gcggcttgct cggccaggcc
     5161 caactggttg cacttggccg ccagcgcttg aagctcgtcg gcagcggcgg gaagagaaaa
     5221 gaaccaaaga accaaagaac caaagaacca gatttcggtt cttcggttct ttggttcttg
     5281 gagtttccgc cccatccctc actcctccac ccggtagatc gccgagcggg tgcgcagaat
     5341 gagggccttg ccggtgacgg cggggctggc catgaagccg tcggcaaact ggttcttggc
     5401 cagctcggtg tactcgcgat cggccttcac gacggtggtg atgccgtcct ggccgcaata
     5461 gtaaatcttg ccgtcggcat aaaggggcga agcgctgaac tcgccgctca gccgcttgct
     5521 ccacagctcc tctccggtct tggcgtcgat gcaagtggcc acgccgggat tgtcatcgac
     5581 gttaaagatc aagtcgccga cgaggacctg cgagggcttg ctgccgatgc tcttcttgct
     5641 ggcccacacg acgtgcgtcg tcgtgacgtc gccctcgccg tcgggcttga cggccaacag
     5701 gtccgccgcg ccgaagcccg tgttgacgta gaccaggccg tggccaaaga gaggcatcgc
     5761 cgcgccggag tggctcgcat agcggacccg ccagaattcg cggccggtcc gcgggtcgta
     5821 cgacatgcaa gccttgctcg aggggctgat cagctgcgtc ttgccagccg cctcaatcac
     5881 cagcggcgtg caaaagctct tcatcaaatc gccgttggtc gtgccgtagt cgatgttgcg
     5941 gtccatcttc cagacggtgt tccccgtctg cttgtcgagg gccacgatgt agtggaagtc
     6001 gtagccgtcc atgtggaaga tcagcaggtt ctcgaacagg atcggcgagc tgccggggcc
     6061 gcggaagtgt tcgcaaggga ggtcgcggcg ctgccagaga gtcttgccgc tggccgtatc
     6121 gaggcaggcg gtgccgtagc tgccaaagtg cacatagacg cggccttcct cgatcgccgg
     6181 cgtgcaggag gcgtagctgt tgagggcgtt gcccagcgga tagaccttct cattgtccca
     6241 cagcttgagg tcgtggacga tcttgccggt gttcttgtcg atgcagatgg cgaagtcggt
     6301 cttgccctct tcggtcgccg cggtgaccca aatctggtcg ccccagatga ccgggctcga
     6361 ccagccgcgg tcgtgcagcg gcgtcttcca cttcacgtgc tccttctcgc tccaggtcgt
     6421 cggcaagccc gtgcttttcg aggtgccgtg gccctgcggc ccgcggaact gcggccagtt
     6481 gtcctgggcg gcgagcggca aggcggccag ggcgatgccg agaatggcaa gtcgatacga
     6541 catgagcgct ccggtcagaa atgggcaatg gggcgggagc ctgattatag tcaaacgctc
     6601 gacgccggac gacgcgctag gccgcgcctt cagcgggctt cccctgtgtt tctggcggcg
     6661 ggctgcgctt cggcttgtag atctcggtcg cggtgccgaa gaacgcctcg ccggcgttca
     6721 tcagcgtttc gctgagcgtc ggatgcgcgt ggatcgactc ggtaatatca gtcacttcgc
     6781 agcccatctc gatcgccagc acggcctcgg cgatcagttc gcccgcgccc ggcccgacaa
     6841 tgccgacgcc gagcacgcgc tcggtctcag gctcaatgag gatcttggtc agcccctcgg
     6901 tgcggcccaa ggcgatcgcc cggccgctgg cggcccaggg atagacgccc gtttcgtacg
     6961 ggcggttttc ctgcttggcc tgctcttcgg taagcccgca ccaggcgatc tccggatcgg
     7021 tgaagacgac cgcgggaatc gccagccggt cgaaggccac gggcttgccg gccacgacct
     7081 cggcggcgac gcggccctca tgcgtcgcct tgtgggccag catcggctcg cccgccacgt
     7141 caccgatcgc caggatgtgc ggatcggctg agcgcatcga acggtctacc ttcacaaatc
     7201 ccttggcgtc gatctcgacc tgcgtgttct ccagcccgaa cccgcggctg ttgggccggc
     7261 ggccgaccga gacgagcacg cggtcgtatt gctcggtgcc gaacttgccg gggccttcga
     7321 aggcaacttc caccttgttg tctcggaggc cgagcgagcc gaccttcgta ttaaggaaga
     7381 ttcgctcgcc gaagagcgta ttcaggcgct tgtgcagcgg tttgaccagg tcgcggtccg
     7441 cgcctggcaa gaggccgtcg agcagctcga cgacgctcac ctgcgagccg agattggcat
     7501 agaccgtgcc catctccagg ccgatgtagc cgccgccgat caccaggagc gtctgcggga
     7561 tctccgccag cgctagcgcg ccggtcgaat ccatcacgcg gtccgaaccg atattgaacg
     7621 cgggcggcat cgcgggcgtc gaaccggtgg cgacgatgca atggtcgaac gtgagtttgc
     7681 ggtccgacgg aatcgaggcg tcgtccccat ccaggcggag tgtcgtgccg ttctcaaaga
     7741 ctccgcgggc gcggatcacc cgcaccttgc ggcgcttggc gatctgagcc agtccgccgg
     7801 agagcgccag gatgaccttc tccttccgct cgcggacttt gttgacgtcg agcttcgggc
     7861 gcggatattc gatgccccag tcgtgccgca gttcctcgac ctcgctgatc acgcgggcca
     7921 cgtgcagcaa tgccttcgac ggaatacagc cccttagcag gcaggtgccc cccagccgcg
     7981 aatcggcctc gacgagcgtg acctccagcc cctcatcggc cgccaaaaat gccgccgcat
     8041 agccgccggg accaccgccg agaacgacga gacgcgaatg catggaagtc aaaagtcaaa
     8101 gaaccgaaaa accaaagaac caaatagggt tcctcagctc ttcggttctt tcgttcttcg
     8161 gttctttggt tctttggaac tttggttctt tgtcaccttg ccaggaactc gacgacgatg
     8221 ttcacatcga ccgggaggct gaagtcgccg gggccgggca ggccgaccat ggtgattcgc
     8281 aggctcgtcg ggtcggtcgt gatccagtcg ggagtctgca cgttgttgcc gtcgccgacg
     8341 acacccttgt atagtccgta gatctgctcc ttcggccgga tcgtgatcac gtcgccgggc
     8401 cggagctgat agctggggct ggtgaccttg atgccattga cgtagaagtg gccgtgcacg
     8461 attccttggc gggcctgcgg gcgggtcttc gtcaggccgc cgcggcggac gacgttgtcc
     8521 agccgccgct cgcagagggc cagcatctgg gccccggtgt cgcccttctt ggccacgacc
     8581 atgcggtaat agcggcgaag ctgcttctcc atcaggccgt agtggtgctt gatcttctgc
     8641 ttctcgaaca gcgccagacc ccagttcgac atcttccgcc cgcccttgac gtgcatgccc
     8701 ggcgggttgt cgcggcgctc cagggcccgg gccgcgcccg ccgtttcata aaccagcgtc
     8761 ttgaggcggc ggttgatccg cgccttggga ccgttgtaac gagccatgcc agtgcagctc
     8821 cctgactgct aaatctcttg ccggcaacaa cttccgcggc tgccattcag ccgcaaagcg
     8881 attggtggaa gctgcttatg attgcggaat cgcgcctccg tggcaagggg tgccccgcga
     8941 ccgtcgcggc cttcgcggct gcatcgtaaa atgagccggc ccaatcactt ggaaccgcca
     9001 tgcccctgga acgcctcgga ccgtacaagc tcgaaaaagt cctcggccgc ggcggcatgg
     9061 gggccgtcta tgtcggcctt aatgacgaga ccggcgagcg ggcagccgtc aaagtcctct
     9121 ccggacacct ggccgacgac accaacttcc gtgaacgctt caagcaagaa atcgagacgc
     9181 tcaagcggct gctgcacccg aacattgtgc agctcttcgg ctacggcgag gaagacggcc
     9241 acctgttcta cgtgatggag ctggtcagcg gccgcagcct gcaggaagag atcgccgccg
     9301 gccgccggtt ctcgtggcgc gaggtcgcgc gaatcggcgt gggcgtcgcc caggccctca
     9361 agcacgccca cgaccgcggc atcatccacc gcgacctcaa gccggccaac ctgctcatgg
     9421 acgcgagcga ccatgtcaag ctcaccgact tcggcatcgc caagctctac ggcggcacga
     9481 atgtcacggc cgaaggcggc gtgctgggaa ccgccgacta catggccccc gagcaggccg
     9541 aggggaagca gccgacgagc cgctgcgacc tgtacgccct gggcagtgtg atgtacgcgc
     9601 tgctcacggg ccggccgccg ttcgtcgggc ggacgatcta tgaggtgatg tccgcgctgc
     9661 agaaggagcc gcccacgcca gtccgccggc tcgcgcccga cacgcccgcg gagttcgaga
     9721 acatcatcct gcagctcttg cacaaggacc cgcaccagcg aattcccacc gcgctggccc
     9781 tggccaaccg cttgaaagcg atggagcacg ccctgtcgct cgaaacgcgc gtgctcgacc
     9841 cgctcgacga tcagccggag gtcgcagagc cgctgcccga cgaggcgctg ccgtcgagcg
     9901 ccaagacggc gtcggcaaac gtgaacgcca acccgacgct gcccatcgag ctggcccaga
     9961 gcgacgaata ccgcctggcc agcgacgcgc cgacgctcgc gaccgacgcc cgcggcggac
    10021 agtcgaacct cactcggctc ggcacgcaag ccgataaacg caccggcgcg ggcgaaagcg
    10081 gccgcaagac cgactctgac cagccggccg ctccgagcaa cactgcggcc gcggcgctgg
    10141 cccgcttcac gaccgtcagc gaggccgagc tccgaggcgc gcgcgagacc gacgaaacgc
    10201 cggtgcggca gtggctgctc gtgggcgggc tcttggcgct ggcggcggtg ctgatcgcgg
    10261 gggtggcgct gttggcgacg cggcccccgt cggccgatcg gctgtttgcc tccgtgcaag
    10321 cggcggccga cagcggcccg gacgagctgg tcctccacga aagcgatttg gtgcagttcc
    10381 tgcagcacca ttcgtccgat ccgcgagcag cggaagtccg cgcctggcag gacgacatgg
    10441 agcaatacca gctgcaaaag cagttggagc ggcgggccag acgccccagc ggcgcggaag
    10501 ggctgacgcc tgccgagcgg gcttacctgg cggcgctgcg gatggagtcc agcgacccgg
    10561 aggctgcgct cgcccgctac gaggcgatcg ccagcgtcta tggcggcccc cccgatccgg
    10621 cactcaaccc gatccaacag cgcatcagcc agcagtgcat cgcattggct cggcaagccg
    10681 cagagcggtt gcggccaact gccgccaagc tgaacgccga ggagaaacag gccgtccgtc
    10741 gtcagttgga gcgggccgac aaactcgcca ccaccgaccg cgccgccgcc gagaaagtct
    10801 ggcgcggcat cgacacgctc tacgccagcc agccctgggc cagcgacctt gtcgccgagg
    10861 cccgcgccaa actcgccgaa tcgccgtaga tcgatcagcg gaaccacgga acatacggag
    10921 tacacggaaa ggcgatctga atttacttcc gtgttttccg tggttcgtct cttgcgccag
    10981 tagcgacccg ctgccgccgt ttgctctaat cggctcgtca tctgtcgccc atctccagga
    11041 gcctcttcca tgaaacgtcg tgcgctgctc gttgtggccg tcctcggttt gctcgctctc
    11101 ggctggctcg ccgaatcgca ttttgccgcg gggcagaacg aagcggcggc gacgatcaaa
    11161 ggctggaagg cgggcgtggg ctggggctgg atctggggca aagacgacga ggtcggggcg
    11221 ctgaacgcga tgaccgacca gtcgcgggcc gcggccctga aattggcggc caccgggaag
    11281 gtttacgacc tgggcatcgc ctacagccgg cggagctata agtggccggg ccactcgccg
    11341 ggcgagatca tgtcgttccg ctcgccggcg ggcgaagagg cgcagaagga gagcgtggtc
    11401 aatcccatgg ggaattccag cgtgacccgc tggcacagca acgctctgtt catgaatgac
    11461 aacgtggcga cgcagatcga cggcctgggc catatcacga tcagcgcgga ccaccactgg
    11521 tacaacggct tcaaggaagc cgagcacggc ggcgactttg gcattcagaa gtgcgacaac
    11581 gtgtcgattc cgccgatcgt cgcgcggggc gtcttgctcg acatcgcggc cgcccgcaag
    11641 gtggacgagc tgccgactgg ctatccgatc tcgccggctg acgtcgatgc cgcgctggcc
    11701 cagcaaaagg tttcactgcg gcccggcgac gtcgtgcttg tccgcaccgg caccctgcgg
    11761 cactggggaa ccgacggcga caaccacgcc aagatcggcc agcacgattc ggccggcatc
    11821 acgctccaga ctgccaaata cctcgtcgag cggcacggcg cgatgctcat cgccagcgac
    11881 accagcggcc tggaggtcaa ccccgcccct gccggcagca actcgttcat ccccgtgcac
    11941 aagtacctgc tggtcgagca gggcgttcac atcggcgagt tccactacct ggaagacctc
    12001 gctcgcgaca aggtctacga attctgctac gtcgccaccg tcaacaagat ccaaggcgcc
    12061 gtcgccggct tcacccttcg gccgctcgcg ctgcgctaat gactatcccg ccgtccgcca
    12121 ggtggccagg cactggcgac acgccagatg tctgaggtgg tccgtcaagc atagcgggct
    12181 cctgtgatcg acaggttttg aacgggttag gaactggcgg tgggaaggaa ggtgagcagc
    12241 ggggcgctgt ggcgacggag cgcgtcgagg tagcgttgtt cgtggtaggg cgtgttggtc
    12301 ttccagcagc ggaacaggat gcgaatccat ttgaaggcga gcgtgcgcag ggcggcatga
    12361 tggcctttgc cgacagcccg ttgctggaga tagaacgccc gggcccagac cgagcgtttg
    12421 ctggagcagt aagcgaactc gtgaaaggtc tggagcagga acttgggaca ggcccagcgg
    12481 cggcggaccg tagagcgctt gccgctgcgc tcggtcaccg gcgcgacgcc ggagagggtc
    12541 tggagctcgg ccgcggattg gaaccgctcg cggtccgtgc cgaaggccgc cagcagccgc
    12601 ggcgcgagcg ctgggccggc gcccggcagg gcgcggaaca aggccgcgtc ggggtgggcc
    12661 tccatcagct cggccaggcg ggcttcgtac ttggcgatcg ccttgtgcag agcgcggagc
    12721 tgggccacca ggcactgcac ctgcagcgtg ccggccccga ggagcgccgc gtcggtggtc
    12781 agcggcaccg cggaccgggc cagctccagg cgctcggcga tccgctcctc gctgcgacag
    12841 gaatgggcgt aatagaactt gcggagcgtc gcaggcagag ccgcttgcac cgcggtcagc
    12901 gtgggccact tgctgaggaa gtcgcaggcc agcggcgtat cgacatcctc cccgaccagc
    12961 accagagcct gtgggtaata ccgcttcagc acggccgtga gctggttgga gaggcgcgtc
    13021 cgctggttga cggcatcgcg gcgggcctcg gccagccaga gcagctcgcg cgtcgctggc
    13081 gaatcgggcc gccacacgcg gagctgatcc cggtgtcgca gcagatagtc cgcgagcagg
    13141 gccgcatcgg tcgggtcgtc cttggcccca ctcgtggaga acgactcgcg gtagcttttc
    13201 agccgggccg gcggcaaggg gaacagcacc aggaagtcgt acttcagcag cgcgtaggcg
    13261 atggccccgc gcgtctgctc gaagcagacc gccaccggcc ggccgccgaa ccgctcgcac
    13321 aggctgcagg cccactcgtc gatggcttcc ggcgtgtgtg gaagctgcga gtgctcgggc
    13381 tttccgcccc gcggatcgta aagcaccagg tcgtgcttct ggtccgccca gtccaggccg
    13441 acaaaggcgg cgaacaccga ggcatccgga ggaacaggtt ccgagacggc gatcatggtt
    13501 tttcactcca gcgggatggg gggcacgcag gaaggaacac gctggctttg gctgggcgaa
    13561 gtacttatag atagacgtgc cgggtgtggc cgtctctgtc tgagcgattc gttacgagcc
    13621 aaagcgcatc gccccgcgcg aagaatctgg cgtaaaacgt gcgaccgttg gtgccactcg
    13681 ttcgtcgcgg ggcgatgcgt gtttcctccg gcgcatcaac ctagcgatgg cgaccagcca
    13741 cctggtgcgg atccgccaaa tctcatccgc aaatttctcc cgcgccctgc cttccggtga
    13801 cttgggaaga gtccctccag gcaggccggg gagaactttg aggaactata agtgccacca
    13861 gccgcgtgcg gtcgtcggca tagccgagca catcggcttt gatggccacg tcgccggctg
    13921 tcctaaaggt gtgcgtcatc tttggagcaa agtcctcggc agcagtacca cctgcccaaa
    13981 ggccaagcac caccaagacg attgccactc gcgtcgcagc ctcccgcaaa ttgcggcgaa
    14041 caggaacgtc gttgtaccgc cttccgttct tgtcgtcact tcgtcactgc gctaagtccc
    14101 acatcctgcc ggcactctca atctatgatg tacatgaaat acaatgtagg cagtttcgga
    14161 ctagggttgc caccacgatg cgttactgcc ttcgcacgct actggcgtgc agtgtaatcg
    14221 cgccgattct gattcagggc ttttgttact ttcttggctg gatttcaatt tgatggaaac
    14281 tctgccggaa ttcgccggac gcttgcttcc agctgttcta ttcgcgtgtc tggcgttgta
    14341 cttcctcatt cgaggagcaa cagccggacc gggctcgcgc cgcttggcaa accttgcgct
    14401 cttctgcggg ttgacaggat tggtcctgcc ggagatttcg ctcggccttt cgcccgctgc
    14461 taactcggcg ttgtttctgg cgtctggtgc gattcgaatc tttttgggag tggcgggcat
    14521 cacgctagcg gcggcagcgc tctggacgcg gggtgacggc ggcgtaggca ttgctcgacc
    14581 cgtgaccgca gccggtttca gttttttgca tgccttcatt ggcgcgggtc tggtgttgtt
    14641 tagctccttt acgtgggcct cgacgccctg ggactttcag tcgccggacg gcgcattccg
    14701 tcttcgtctt ccctcacagc agtggaagca agcgccggtt gacgaccgcg cggatgccat
    14761 cgccttcgtt tgttcatcac ctcggatgca agctcgcgta gtggcagtaa ggcaagaaca
    14821 aaccgcggcc gactttgccc tggccgtcga ggcgttgcgt gcgcggcttg acagcttgcc
    14881 cacacgacgc gacacggccg aagaccggga aggtacgaca acgtcaggcg ggtcctatcg
    14941 ttacgtcaca tttatggact cgacgcccga cggcaatccg gtgttcgtgg cctactccgt
    15001 aacttggtgt ccccggcagc aaaccgtaat cgaagtgctc gtcgaaggcc tacccaggtt
    15061 gctatcgcaa acaggcaagg caggcgagat gcaccgcttc cagaaatccg ccgagacaat
    15121 ttgcctgtct gtggaataaa ggtttgccca agattgcttc cggccaatga atgaaagagg
    15181 ctgtacctag ctacagcagc tcggggatcg gcgtgcccga ag
//
LOCUS       DASRJB010000062         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3407423, whole genome shotgun sequence.
ACCESSION   DASRJB010000062 DASRJB010000000
VERSION     DASRJB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3407423"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFB80_01735"
     CDS             complement(<1..790)
                     /locus_tag="VFB80_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32501.1"
                     /translation="MLRHVVAASLFIFAALAAGCSCNSGVGPAPVGTNGGRVPVAGDP
                     DVIVVEHTDYRLPPAADDGLADDRLEDKKTAFDPELVDRRPEAGWSINQSEAVVRLDV
                     PLVRPDQEQDLLTLRPSYAAALQAARQTRSWIPALPSVNLLDGKAKQFDDGLYAAIDQ
                     AWFEGVEAKVQSHLSLVERLLQKVGPDSAAAPHLAAALTLGGKETSVADNPARDALIQ
                     KFNANPALAKPIAFYTWNPTLQRCWAFMRFLQQSQKESDPAVAAI"
     gene            1180..2181
                     /locus_tag="VFB80_01740"
     CDS             1180..2181
                     /locus_tag="VFB80_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HZN32502.1"
                     /translation="MKYAAVGPIAVHLPERVETNAQLKAEFPGWDMDLIYEKTGIRSR
                     HIAAEGECASDLAAAAAQKLFQQYDVDPASIDFLLLCTQTPDYPLPTTACLLQSRLGL
                     KTSVGALDFNLGCSGYVYGLSLADGLIRSGAARRVLLLTAETYSKYIHPTDRSLRTIF
                     GDGAAATLVEAASEPTLTAFQFGTDGTGADTLLVTKGGARPPEQAIQPRHRQRWESAL
                     YMDGPSLINFTVVAVPQLVENILAAARLSDKDIDLYLFHQATLKMLEQLRERMRVKPE
                     RLPMVLEHCGNTVSSTIPILIEHLRKNGQLKRGMRSMLVGFGVGWSWAGGIWRETFS"
     gene            2287..2427
                     /locus_tag="VFB80_01745"
     CDS             2287..2427
                     /locus_tag="VFB80_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32503.1"
                     /translation="MKVGEFDYRNAKAVLQATVPEHLQEIYSILQRPEHNLDLMKGQQ
                     QR"
BASE COUNT          420 a          799 c          794 g          418 t
ORIGIN      
        1 cgatcgccgc cacggccgga tcggattcct tttgcgattg ctgcaggaac cgcatgaagg
       61 cccagcagcg ctgcagcgtc gggttccagg tgtagaacgc gatcggcttg gccagcgccg
      121 ggttggcgtt gaacttctgg atcagcgcgt cgcgggccgg gttatcggcg acggaggtct
      181 ccttgccgcc gagcgtcagg gccgccgcca ggtgcggcgc ggccgcgctg tccgggccga
      241 ctttttgcaa gagccgctcc acgagcgaca ggtggctttg caccttggcc tcgactccct
      301 cgaaccaggc ctggtcgatg gctgcataga gcccgtcgtc aaactgcttg gccttgccat
      361 cgagcaggtt gaccgacggc agcgccggaa tccaggagcg agtctgtctc gctgcttgta
      421 gagcagccgc atacgacggc cgcagcgtca gcaggtcctg ctcctgatcg ggccggacga
      481 gcggcacgtc gagtcggacg acggcttcac tctggttgat gctccagccg gcttccggcc
      541 gccgatcgac cagctccgga tcgaacgccg tctttttgtc ttctagccgg tcatcggcca
      601 ggccgtcgtc ggccgccggc ggcagccggt aatcggtgtg ctcgaccacg atcacgtcgg
      661 gatcgcctgc aaccggcacg cggccgccgt ttgttcctac gggcgctgga cctacgccgg
      721 agttgcagct gcagcccgcg gccaatgcgg caaaaatgaa gagtgaggcg gcaacaacat
      781 ggcgcagcat ggcacgtctc cgaagcgtga acgaggcttc cgaccgattg agcccggctg
      841 gcaacgaggt attccccaaa aaacggccga tggtctgtca gtcggtcggt ggtcagtagg
      901 tccgtggtca gtgcgtcgga tcattccacc cagcgcactg agtactgagt acggagtacg
      961 gagtcggtaa ggatgctgcg tacgaagccg cgtttcaccg accaccgacc gaccgactta
     1021 gcgaccaccg atctaccgct gtcgccagcg ccaattgacc cgcgccgcgg cccccgttag
     1081 gctaaacttt ttgctcatcc agcccaggcg gcgcgggacg gagcccgaac aagcgctccc
     1141 tcacgatgcc gcgggccttt cccgacaggt ccggcgtccg tgaaatacgc agcggtcggc
     1201 cccatcgccg tccatcttcc cgagcgcgtc gagaccaacg cccagctcaa ggcggaattc
     1261 cccggctggg acatggacct gatctacgaa aagacgggca ttcgctcccg ccacatcgct
     1321 gccgaggggg aatgcgcgag cgacctggca gccgcggccg cccagaagct gttccagcaa
     1381 tacgacgtcg atccggcgtc gatcgatttc ctgctgctct gcacgcagac gcccgactat
     1441 ccgctgccca ccaccgcctg cctgctgcag agccggctgg gtctgaagac cagcgtcggg
     1501 gcgctggact tcaacctcgg ctgctcgggc tatgtctatg gcctgtcgct ggccgacggg
     1561 ctgatccgca gcggcgcggc ccgccgggtg ctcctgctga cggccgagac ctattcgaag
     1621 tacatccatc ccacggaccg cagcctgcgg acgatctttg gcgacggggc ggctgcgacg
     1681 ctggtcgagg ccgccagcga acccacgcta accgcctttc agttcggcac cgatggcacg
     1741 ggcgccgata cgctgctggt caccaagggg ggcgcgcggc cgccggagca ggcgatccag
     1801 ccgcggcatc gccagcggtg ggagagcgcg ctgtacatgg acgggccgag cctgatcaac
     1861 ttcacggtcg tggcggtccc gcagctcgtc gagaacatcc tcgcggccgc caggctgagc
     1921 gataaggaca tcgacctcta tctgttccac caggccacgc tgaagatgct ggagcagctc
     1981 cgcgagcgga tgagggtcaa gccggagcgg ctgccgatgg tcctggagca ctgcggcaac
     2041 acggtcagct cgacgatccc gatcctgatc gagcacctcc gcaagaatgg ccagctgaag
     2101 cgcgggatgc ggagcatgct cgtcggcttc ggcgtcggct ggtcctgggc cggcggcatc
     2161 tggcgcgaga cgttctcgta gcattggcgc ccccgtttgt gccctccgtt tccgcgggcc
     2221 gtattccgac gctgccgtcc ggcagatcga ggggaactgg gggcggtttg gctcttggag
     2281 acgtggatga aggtcggcga attcgattat cgaaacgcaa aagcagtcct tcaggcgaca
     2341 gttccggagc atctacagga gatctattcg atcctccagc gtccggagca caatctcgac
     2401 ttgatgaagg ggcaacagca gcgatagctt t
//
LOCUS       DASRJB010000063         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2843562, whole genome shotgun sequence.
ACCESSION   DASRJB010000063 DASRJB010000000
VERSION     DASRJB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843562"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFB80_01750"
     CDS             complement(<1..437)
                     /locus_tag="VFB80_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32504.1"
                     /translation="MSRLVALEWDAKEARVVVGRKRGTALVVEQAFAAPLPQKAEGSA
                     AEADIGGAIAKALSERGLSRVEALVAVGRASIELKFLSTPPVPPEELPELVRFQALRQ
                     FTSLGDDWPLDFVPLGPTADGGTNVLAAAISADLVGQMKKTCSA"
     gene            complement(516..2039)
                     /locus_tag="VFB80_01755"
     CDS             complement(516..2039)
                     /locus_tag="VFB80_01755"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32505.1"
                     /translation="MKPTRRGLILVLVLIVIAFLSLGAYSFTDLMLAQHDAAIQYGRQ
                     AAARGLVDTGAAAVQIFLSQPEADRANAGGIFDNAERFQGVTVMEDADPKLRGCFSVM
                     SPNIDSNGNLAGVRHGLEDESTRLNLNTLLAVDKLLPGSGKTFLMALPGMTEDIADAI
                     MDWLDTDEEPRELGAEIEYYSGLSPPYAPKNGPLETVEELLLVRGVTPELLFGVDVNR
                     NGQLDTHESQQGGDVDPTAFRGWSAYLTLHSLEWNVTPEGEPRIYINNNDLTALHEQL
                     SLVFPPEWADFIIAYRQMGPVTSTSQVTTTSSSSVKIDLSLPAKQPLTQVLDLVGASV
                     RYNQTDGAGNSIVAVMPSPFGATLGQMAGYMPKLMDYVTVNPAATIPGRININQAPVT
                     ILNGIPGMRPEVAEQIVSSRDVESKNDSRRHETWLLQEGIVTLEEMKTMMPFVTCGGD
                     VYRGQIVGYFQGGAASSRAEIIFDATTPLPRLLFWRDISHLGRGYAVETLGVDFAEP"
     gene            complement(2036..>2629)
                     /locus_tag="VFB80_01760"
     CDS             complement(2036..>2629)
                     /locus_tag="VFB80_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32506.1"
                     /translation="ISRLPRVDQYDPQYAAGGEVAATDIPSDVKTVVYFLADEGMPPD
                     AKVIGPQDVLPSATGMGKGLMRRELDRAVNSYAESNGDTTSIYSGARLVSDKVVGIQF
                     QYFDGSAWLPEWDSDASGGLPRAVEIVLTVQPTFGMTEEEIAELAPDQVPPVRTYRQV
                     MKIPTASLAPPATTEATTETGTAAPASGTPSTQGATP"
BASE COUNT          435 a          834 c          886 g          474 t
ORIGIN      
        1 gccgagcagg ttttcttcat ctggccgacg aggtcggccg agatggcggc ggccagcacg
       61 ttggtgccgc cgtcggcggt cggacccagc ggcacaaagt cgagtggcca gtcgtcgccc
      121 agcgaggtga actgccgcag ggcctggaac cgcaccagct cgggcagctc ctcgggcgga
      181 accggcggcg ttgaaagaaa tttcagttcg atgctggccc gtcccaccgc gaccagcgcc
      241 tcgacccgcg acaggccgcg ctcggagagg gccttggcga tcgccccgcc gatgtcggct
      301 tcggccgcgc tgccttccgc cttttgcggc agaggggcgg cgaaggcctg ctccacgacg
      361 agagccgtgc cgcgcttgcg gccgacgacc acgcgggctt ccttggcgtc ccattccagg
      421 gcgactagac gcgacatcaa cgcaccctta caggctcaga ttcccaagca ttcaaccatc
      481 gcgggcggcg aaagttccgc aacatgttgc gggaactatg gttcggcaaa gtccacgccc
      541 agcgtctcga ccgcatagcc gcggcctaga tggctgatgt ctctccagaa tagcaggcgg
      601 ggcagcgggg ttgtagcatc aaagatgatc tccgcccgac tggaggctgc gcccccctgg
      661 aagtagccca cgatctggcc gcgatagacg tcgccaccgc aggttacaaa cggcatcatc
      721 gtcttcattt cttcgagcgt gacgatcccc tcctggagca gccaggtttc gtgccggcgg
      781 gagtcgttct tcgactccac gtcgcggctg gagacgattt gctcggcgac ctccggccgc
      841 atgccgggaa tgccgttcag aatggtcacc ggggcctggt tgatgttgat gcggccggga
      901 atcgtggccg ccgggttgac cgtgacgtag tccatcagct tcggcatgta accggccatc
      961 tggccgagcg tcgcgccgaa gggggagggc atcacagcca caatcgagtt gcccgcgccg
     1021 tcggtttggt tgtagcggac cgacgcgcca acaagatcca agacttgcgt cagcggctgc
     1081 ttggcaggca gcgacaggtc gatcttcacg gaactgctgc tcgtcgtcgt gacctggctg
     1141 gtggaggtaa ccggccccat ctgccggtag gcgatgatga agtcggccca ctctggcgga
     1201 aagacgagcg acagttgctc gtgcagggcc gtcagatcgt tgttgttaat gtaaatccgc
     1261 ggctcccctt cgggcgtgac gttccactcc aggctgtgca gcgtgaggta ggcggaccag
     1321 ccgcggaagg ccgtcggatc gacgtcgcct ccctgctggc tctcgtgagt gtcgagctgg
     1381 ccgttacggt tgacatcgac accaaacagc agctcgggcg tgacgccgcg gaccagcagc
     1441 aattcctcga ccgtctccag cgggccattc ttcggcgcgt agggcggact caagcccgag
     1501 tagtattcga tctccgcgcc cagctcgcgc ggctcttcgt cggtgtcgag ccagtccatg
     1561 atcgcgtcgg cgatgtcctc ggtcatgccg ggcagagcca tgaggaaggt cttgcccgag
     1621 ccgggcaaca gcttatcgac ggccaggagc gtgttgaggt tgagacgtgt cgattcgtct
     1681 tccaggccgt gccgtacgcc cgccaggttg ccgttgctgt cgatattggg cgacatcacg
     1741 ctgaagcagc cgcggagctt cgggtcggcg tcttccatca cggtgacacc ctggaaccgc
     1801 tcggcgttgt caaagatgcc gccggcgttg gcccggtcgg cctccggctg cgaaaggaag
     1861 atttgcaccg cggccgcgcc ggtatcgacc aggccccggg cggcggcctg ccggccgtat
     1921 tgaatggcgg cgtcgtgctg ggccagcatc aagtcggtga acgagtacgc gcccagcgac
     1981 aggaacgcga tcacgatcag cacaagcacg agaatcaggc cgcggcgggt cggcttcatg
     2041 gcgtggcccc ctgtgtgctg ggcgtgccgg aagcaggagc agcagtcccg gtttccgtcg
     2101 tcgcctcggt ggtcgccgga ggggccaggc tggcggtcgg aatcttcatg acctggcgat
     2161 aggttcgcac gggcggcacc tggtcgggcg cgagctcggc aatttcttct tcggtcatgc
     2221 caaacgtcgg ctgcacggtg agcacgattt cgactgcccg gggcaggccg ccggaggcgt
     2281 cggaatccca ttctggcagc cacgccgagc cgtcgaagta ctgaaactga atcccgacca
     2341 ctttgtcgga gacgaggcgg gccccggaat agatcgaggt ggtgtcgccg ttggactcgg
     2401 cgtaggaatt gacggcccgg tccaattccc ggcgcatcag gcccttgccc atgcccgtgg
     2461 cgctgggcag gacgtcttgc gggccgatca ccttcgcgtc gggcggcatg ccttcgtcgg
     2521 ctagaaaata gacgactgtc ttcacgtcgc tgggaatgtc cgtggcggcg acctcgcccc
     2581 ccgcggcgta ttgcggatcg tattgatcga cccgcggcag ccggctgat
//
LOCUS       DASRJB010000064         6020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2561145, whole genome shotgun sequence.
ACCESSION   DASRJB010000064 DASRJB010000000
VERSION     DASRJB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561145"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFB80_01765"
     CDS             <1..333
                     /locus_tag="VFB80_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32507.1"
                     /translation="ALALLARQESQRLESALARAGSVEAPAPRYTLAAIESLGDAARL
                     DDEERLPQLAAQISLAPGIRGELFDDNRDLVAVANLADQGLSAPEIARRLSLPIGDVE
                     LLLNLRSA"
     gene            complement(330..701)
                     /locus_tag="VFB80_01770"
     CDS             complement(330..701)
                     /locus_tag="VFB80_01770"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZN32508.1"
                     /translation="MPRSWLDKFRDAFRGLWLAVRSERSFVVHLPMVAAVGVLAFLLR
                     VSLVEACLLGLCVAIVLAAELFNTALERLARAIDCEENDHLAAALDIASSAVLIAALG
                     AAGVGGAIFAYRAGALAGWWP"
     gene            862..3285
                     /locus_tag="VFB80_01775"
     CDS             862..3285
                     /locus_tag="VFB80_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN32509.1"
                     /translation="MACCRLFALPVVAALLLSRVALAQAPPAEAKPAETRPAEAKPAE
                     AKPDEAKPDEKKPEDKKPEEKKPDDKPAESPTTAAGPLPGHSYHGEVFDEGPRQKAYL
                     MEGMPKIHFPVTTKNPEVQKFIEQGVGQIHGFWYYEAERSFRQAATLDKDCALAYWGM
                     ALANIDNANRAKKFMAECVKHKAGISDRETMYIDAADAYFKADNNKRKERNEAYTKAL
                     ERILYKYPDDIEARAFLALQLWKNRDGGTPIASFLAIDALLDQVFKADPMHPAHHYRI
                     HLWDTEKAENALGSAALGGQSSPGVAHMWHMPGHIYSRTKRYEDACYQQEASARVDHA
                     HMMRDRVLPDQIHNFAHNNEWLIRDMIFVGRMRDAIDLAKNMCELPRHPKYNTPNRGS
                     ANFGRLRLFESLTRFEAWDELIALAETPYLDATDNDQEQLKRQRHLGMAHYRKGDVAG
                     GDAQLAALEQKLADQKAAQEKAGADAEQKAKDDKKDDKAVEKAKNDARKPFDQKINNL
                     TKAVEEVKGHQAVAQGEFKSGLELLRKAGGIDADYLAWVQFKAGDTDKAIEAAQGAVR
                     SRKNEVIPLARLVELQWLAGKKDDAKQSLDQLRELSNSIDKSAPLYARLAPIAQELGF
                     SEDWKATKAPLPDTGVRPSLDSLGPFRWQPSPALSWTLKDDHGADHSLADFHGKPVVL
                     LFFLGHGCLHCAEQVQAFGKAAKDFSESGIELIGISSDDAAGLKQSIENYKDGPVPFP
                     LVSDNALETFKTYRCYDDFEQLTLHGTFLIDGAGLVRWQDISYEPFQDTKFLLTEARR
                     LLGQSADGK"
     gene            3492..3662
                     /locus_tag="VFB80_01780"
     CDS             3492..3662
                     /locus_tag="VFB80_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32510.1"
                     /translation="MFRWSLRSLFILLAVCGVASRVASEAVKSQDQQRILEKLQKLGP
                     LLDEKTLLLAIT"
     gene            3741..3926
                     /locus_tag="VFB80_01785"
     CDS             3741..3926
                     /locus_tag="VFB80_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32511.1"
                     /translation="MRLFHRPTRLALEGPVFTDAAVSELSRLTALKSLTLYRTKVSSQ
                     GLVRLRQALPDCQIERK"
     gene            4118..4285
                     /locus_tag="VFB80_01790"
     CDS             4118..4285
                     /locus_tag="VFB80_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32512.1"
                     /translation="MCRWSLRTLFVVVAICGLASRVAREAALAQEQERVLEKLRGIVP
                     DLRDSRLKCFL"
     gene            4307..4552
                     /locus_tag="VFB80_01795"
     CDS             4307..4552
                     /locus_tag="VFB80_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32513.1"
                     /translation="MTVEFQGPHFLRRPAEAVGIRIFHRPTRLFLRGPDYTDAAALHL
                     ARLTSLQSLTLDGTNISAAAVERLRHALPHCQIEHRH"
     gene            4725..>6020
                     /locus_tag="VFB80_01800"
     CDS             4725..>6020
                     /locus_tag="VFB80_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN32514.1"
                     /translation="MAWIRSCDLDAGRDERPPIPTRIASNEEFIPPPQSPEQREVELR
                     LWAIAERCAGKLGLSRRDFLRTSCGMAAALLAMNEVFGECYAVEPDEPVNPEKVKEKW
                     PKDQFIFDVQTHHVDVRGEWYDDTPDGRWFKNFVRSLRPGGTFDSSLERLNVEHYVKE
                     IFFDSDTVMAIISGVPTREWNKNPLPPDQMAATRDYVNKLAQSQRVLAHGLLRPNLGA
                     KELDEMERQVKDLKISAWKMYTGAEIGEAAWWLDDEKVAYPFWERTQKLGVKNICVHK
                     GLPLGVFNEEHCKPRDVVKAAKDFPDLNFIIYHSGFRGLSLLSRPREAQEIPWISDLL
                     RDLKANDKVKNVYFELGSTFNQLSSYSPETCLHMLGQMIQIAGADHILWGTDSIWGGS
                     PQSQIERLRRLKMTDALIEKFGYAPLTDEVKNQIFGLNAA"
BASE COUNT         1182 a         2029 c         1871 g          938 t
ORIGIN      
        1 gccctggccc ttctcgcgcg gcaggaatcg cagcgcctgg aatcggcatt ggctcgcgcc
       61 gggagtgtgg aagctcccgc gccgcgctac acgttggccg ctatcgaaag cctgggcgac
      121 gccgcgcggc tcgacgatga agagcggctg ccgcaactgg ccgcccagat atcgctggcg
      181 cctggcatcc ggggcgaact gttcgacgac aaccgcgacc tcgtcgccgt cgccaacctc
      241 gccgaccagg gcctgtccgc ccccgaaatc gcccgccgcc ttagcctgcc catcggcgac
      301 gtcgaactgc tgctgaacct ccgctcggcc tagggccacc agcccgcgag tgctccggct
      361 cgataggcga aaatcgcccc gcccacgccg gctgcgccca gcgcggcgat cagcacggcg
      421 ctgctggcga tgtcaagagc ggcggcgagg tggtcgttct cttcgcaatc gatcgcgcgg
      481 gccagccgct ccagggccgt gttaaagagc tcggccgcca ggacgatcgc cacgcacagg
      541 cccagcaggc aggcctcgac caggctcacg cgcagcaaga aggccagcac gcccacggct
      601 gccaccatcg gcaagtgcac cacgaaactc cgttcgctgc ggaccgccag ccacaggccg
      661 cggaaggcgt cgcgaaattt gtccagccag gagcgaggca tggggggaaa ttccacgaac
      721 caaagaaccg aagaaccaag tggagcagcg ttgggttctt cggttcttcg gttctttggt
      781 cctttggctt tttccttgcg gcggcttaga atactggctt gaccgttccc caccaagtta
      841 gccccgcctg gatgcctcct catggcctgc tgccgcctct ttgccctccc tgtcgtcgct
      901 gcgctcctgc tcagccgtgt ggcactggcc caggcgccgc ctgcggaagc caagccggct
      961 gaaaccaggc ccgctgaagc caagccggcg gaagccaagc cggacgaggc gaagcccgac
     1021 gaaaagaagc cggaagacaa gaagcccgag gaaaagaagc ccgacgacaa gccggccgag
     1081 tcgcccacga ccgcggccgg cccgctgccg ggtcattcct accacggcga agtcttcgat
     1141 gaaggcccgc ggcagaaggc gtacctgatg gaagggatgc cgaaaatcca cttccccgtg
     1201 accaccaaga accccgaggt ccagaagttc atcgagcaag gagtcggcca gattcacggc
     1261 ttctggtact acgaagccga gcgctcgttc cgccaggcgg cgacgctcga caaggactgc
     1321 gccctggcct actggggcat ggcgctggcg aacatcgaca acgccaaccg ggccaagaag
     1381 ttcatggccg agtgcgtcaa gcacaaggcg ggcatcagcg atcgggagac gatgtacatc
     1441 gatgccgccg acgcctactt caaggccgac aacaacaagc ggaaggagcg caacgaagcc
     1501 tacacgaagg ccctggagcg gattctctac aagtacccgg acgacatcga ggcccgcgcc
     1561 ttcctggccc tgcagctctg gaagaaccgc gacggcggca cgcccatcgc cagcttcctg
     1621 gcgatcgacg ccctgctcga ccaggtcttc aaggccgacc cgatgcaccc ggcccaccac
     1681 taccgcattc acctgtggga caccgagaag gccgagaacg ccttgggctc cgcggccctg
     1741 ggcgggcaat cgtcgccggg agtggcccac atgtggcaca tgccgggcca catctattcg
     1801 cggaccaagc gctacgagga cgcctgctac cagcaggagg cctcggcccg cgtcgatcac
     1861 gcccacatga tgcgcgaccg cgtgctgccc gatcagatcc acaacttcgc ccacaacaac
     1921 gagtggctga tccgcgacat gatctttgtc gggcggatgc gcgacgcgat cgacctggcc
     1981 aagaacatgt gcgagctgcc gcggcacccc aagtacaaca cgcccaatcg cggcagcgcg
     2041 aatttcggcc gcctgcgcct cttcgagtcg ctcacccggt tcgaagcctg ggacgagttg
     2101 attgccctgg ccgagacgcc ctatctcgac gccaccgaca acgaccagga gcagctcaag
     2161 cggcagcggc atctgggcat ggcccattac cgcaagggcg acgtggccgg cggcgacgcg
     2221 cagctggccg ctctggagca aaaactcgcc gaccagaagg ccgcgcagga aaaggccggc
     2281 gccgacgcgg agcagaaggc caaggacgac aagaaagacg acaaggccgt cgaaaaggcg
     2341 aagaacgacg cccgcaagcc gttcgatcag aagatcaaca acctcacgaa ggccgtggaa
     2401 gaggtcaagg gccaccaggc ggtcgcccag ggcgaattca agtccgggct ggaactgctc
     2461 cgcaaggccg gcggcatcga cgccgactat ctggcctggg tgcagttcaa ggccggcgac
     2521 accgacaagg ccattgaagc cgcgcaaggc gccgtccgct cgcggaagaa cgaagtcatc
     2581 ccgctggccc ggctggtgga attgcagtgg ctggccggca agaaggatga cgccaaacag
     2641 tcgctcgacc agctccgcga gctctcgaac tccatcgaca agtccgcgcc gctctatgcc
     2701 cgccttgcgc cgatcgccca ggagctgggt ttctcggagg actggaaggc cacgaaggcc
     2761 ccgcttcccg acaccggcgt gcgaccgagc ctcgattcgc tagggccctt ccgctggcag
     2821 ccgtcgccgg ctctctcttg gacgctcaag gacgaccacg gcgcggacca ctcgctggcc
     2881 gattttcacg gcaagcccgt cgtcttgctc ttcttcctcg gccacggttg cctgcactgt
     2941 gccgagcagg tgcaggcctt cggcaaggcg gccaaggatt tctcggagtc cggcatcgag
     3001 ctgatcggca tcagcagcga cgacgccgcc gggctcaagc agtcgatcga gaactacaag
     3061 gacggccccg tgccgttccc gctcgtctcc gacaatgcgc tcgagacatt caaaacctac
     3121 cgctgctacg acgacttcga gcagctcacc ctgcacggca ctttcctcat cgacggggcc
     3181 ggcctggtcc gctggcagga catcagctac gaacccttcc aggacaccaa attcctgctc
     3241 accgaagcca ggcgtctgct tggccagtcg gccgatggaa aataggtcgg ccttcggagg
     3301 gtggctgtga accggcggag ggccgtctgc gtatcaccgc ttaccccgga atcctaccgc
     3361 gagatcggcg agcggccggc gtaagccggc cgataactct gccccggaga gacgtcgtca
     3421 tcgggaggcg tacgcctccc gctcgcctgg gggccggggc taagcgaacg aagcatcgct
     3481 ggaggcgtgc catgttccgc tggagcttgc ggagtctgtt catcctcctg gctgtctgcg
     3541 gcgttgcgtc gcgggtggcc agcgaagcgg tgaagtcgca ggatcagcag cgcatcctgg
     3601 agaagctgca gaagttgggc ccgctgctgg acgagaagac gctgctactg gcgattacgt
     3661 gaacgccgaa tagcagcggc cgcgtgacgg tcgaatatca aggtccagaa ttcttgcggc
     3721 gtcccgtgga ggcattgggc gtgcgccttt tccaccggcc caccaggctc gccctggaag
     3781 gtccggtctt caccgacgcc gccgtgagcg agctcagccg cctgaccgct ctaaagtcgc
     3841 tgacgctcta ccgcaccaaa gtctccagcc agggcctggt tcggctgcgg caagcattac
     3901 ccgactgtca gatcgagcgc aaatagcggc tgaaccgctg cgggaccggc tgcgtactcc
     3961 cgcttagccc aggcctccta ccgcgagatc ggcgagcggc ctgcgtaagc cggcagataa
     4021 ctctgccctg agagacgtca tcgggaggct tacgcctccc gctcgccttg cggcaagctg
     4081 cccgccgggg ttcagcgaat caagcgaggg ctattccatg tgtcgctgga gcttgcggac
     4141 tctgttcgtc gtggtggcca tctgcggcct cgcctcgcgg gtggcccgcg aagcggccct
     4201 ggcccaggag caagagcggg tgctggaaaa gctgcgcgga atcgtcccgg accttcgtga
     4261 ttcgcggctc aagtgcttct tgtgaccgtc cgatagcagc ggccgcgtga cggtcgaatt
     4321 tcaaggcccc cacttcctgc ggcgtcccgc ggaggccgtg gggatacgca tcttccaccg
     4381 gcccaccagg ctcttcctgc gaggaccgga ttacaccgac gctgccgcct tgcacctcgc
     4441 gcgcctgacc agtctccagt cgctcacgct ggatggaacc aacatctccg ccgccgccgt
     4501 ggagcggctg cggcacgcct tgccccactg ccagatcgag catcgccatt gagaagaacc
     4561 aaagaaccaa agatccaaaa aaccgaagac cgaactggag agcttataaa cattcagtcg
     4621 ggcctattcc cagcacttgc tgcactccgc agaatactca ctggcactcg ccctttcggt
     4681 tcttcggatc tttggttctt cggttctttt ccccggagct tcccatggcc tggatccgct
     4741 cctgcgatct cgatgccggc cgcgacgagc ggccgccgat tccgacccgc atcgccagca
     4801 acgaggagtt catcccgccg ccccaatcgc ccgagcagcg cgaagttgag ctgcggttgt
     4861 gggcgatcgc cgagcggtgc gcgggcaagc tgggcctctc caggcgagat ttcctgcgta
     4921 ccagctgcgg catggcggcg gccctgctgg cgatgaatga agtctttggc gaatgctacg
     4981 cggtcgagcc cgacgagccg gtgaatcccg aaaaggtcaa ggagaagtgg cccaaagacc
     5041 agttcatctt cgacgtgcag acgcaccacg tcgatgtccg cggtgagtgg tacgacgaca
     5101 cgcccgacgg ccgctggttc aagaatttcg tccgctcgct ccgccccggc ggcacgttcg
     5161 acagcagcct cgagcggctg aacgtcgagc actacgtcaa ggagatcttc ttcgacagcg
     5221 acaccgtgat ggcgatcatc agcggcgtgc ccacgcgaga atggaacaag aacccgctgc
     5281 cgccggacca gatggccgcc acgcgcgact acgtcaacaa gcttgcccaa tcgcagcggg
     5341 tgctggccca cggcctcttg aggccgaatc tcggggccaa ggagctcgac gagatggagc
     5401 ggcaggtcaa ggacctcaag atcagcgcct ggaagatgta caccggggcg gagatcggcg
     5461 aggcagcctg gtggctcgac gacgagaagg tcgcgtatcc cttctgggag cggacgcaga
     5521 agctcggcgt caaaaacatc tgcgttcata aggggctgcc gctgggcgtg ttcaacgaag
     5581 agcactgcaa gccgcgggac gtggtcaagg cggcgaagga ctttccggac ctcaacttca
     5641 tcatctacca ctcgggcttc cgcggcctgt cgctgctgag ccggcccaga gaagcgcaag
     5701 aaattccctg gatcagcgat ctgctgcggg acctgaaggc taacgacaag gtcaagaacg
     5761 tctactttga gctgggcagc acgttcaacc agctctcctc ctactcgccg gaaacctgcc
     5821 tgcacatgct cggccagatg atccagatcg ccggcgcgga ccacatcctg tggggcaccg
     5881 attcgatctg gggcggcagc ccgcagagcc agatcgagcg gctgcggcgc ctcaagatga
     5941 ccgacgccct gatcgaaaag ttcggctacg cgccgctcac tgacgaagta aagaatcaga
     6001 tctttggcct gaatgccgct
//
LOCUS       DASRJB010000065         7161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3971471, whole genome shotgun sequence.
ACCESSION   DASRJB010000065 DASRJB010000000
VERSION     DASRJB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3971471"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2788)
                     /locus_tag="VFB80_01805"
     CDS             complement(86..2788)
                     /locus_tag="VFB80_01805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZN32515.1"
                     /translation="MPAHRIEPGQLAAALGSDIARGLDPSQVSALRSRYGANQLEQAP
                     PVPMWKRFLGQFKEIVIWILIVAAIIAAAMGEVADAIAILAIVLLNGIVGFIQEEKAE
                     QALAALRKLSSPNAKVVRGGKAQQIPAAELLPGDYIELEAGDYVPADARLISAASLQT
                     QEAALTGESSPVEKDPGVTLPEQTPLAERSNSVYSGTVVTKGKAAALVVATGMQTELG
                     NIAGLLARQPPEPTPLQRRLAELGRVLVVVCLALVAVVFVLQLVRGGELLQVFLVSVS
                     LAVAAVPEGLPAVVTLALALGLQRMAARNALVRKLPSVETLGSVTVICTDKTGTLTRN
                     EMTVRRLVAGDEEFEVTGSGYKPEGEFRRAGAGESIKAADQPGLRMLLSVGVRCNGAR
                     LQRSEKENQSWQVIGDPTEGALLVAAGKAGLPFARDDFQVASEIPFDSNRKAMSVIVR
                     ESDGSELICTKGAPEVVLAMCSRELRGGQEQPLDERRRQEVLRKNAQLADNALRVLAL
                     AYRQRQPGERELAEKELVLAGLAGMLDPPRDEARVAVKACRSAGIRPIMITGDHPATA
                     QAIARDLGILNEGDTVVAGQELQATSDETLTNGVHRTSVYARVSAEHKLRIVKAWQRR
                     GEVVAMTGDGVNDAPAVKAAEIGIAMGITGSDVTKEASDMVLTDDNFRSIVSAVEEGR
                     CIFDNVRNIVHYLLSCNAGEVLFMFFAALVGWKTPLAAIQILWINLVTDGLPALALAM
                     ERPDRDVMQRPPRPPHEPVLTRRRGRLIVTHGLLVAAATTVGFWLVYGGRTERFDEAR
                     TVAFCILAFGQLFFAFACRSEQRTLPELGPFSNPYLLGAIAVSGLLQLAVVTLPFARK
                     VFKVDADLTSGDWLLVALLSLAPVTVVEVTKLVRAALRSHAPNS"
     gene            complement(2874..4103)
                     /locus_tag="VFB80_01810"
     CDS             complement(2874..4103)
                     /locus_tag="VFB80_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN32516.1"
                     /translation="MKSAIHLLGMLGILVAAIGSAVGQQPSAPRLNMVGPYGPWLSQT
                     VLGDGPARLSFRTGKWKTLEEWRAKGRERLWECMAPVDLGGRPEVRVESTHEFDGLHI
                     ERLSWQLPGGPRTEAVFLKPAGAKGPLPGILALHDHGGNKFLGWGKIARIDDKPGEVV
                     ADHQQRYYGGVAWANEIAKRGYAVLVHDTFPFASRRVRVDAVPERLRQNGIDPQPDDA
                     EGIKRYNAFAAAHEHIMEKSLLSAGTTWPGVYVVEDQRALDVLCARPEVDAKRVGCAG
                     LSGGGMRTVFLGGLDDRIRCAVAVGFMTTWREFLLDKCFTHTWMAYVPLLPRDLDFPE
                     ILALRAPAATMVLNCTEDALYTVPEMRRADAMLRETFERGGAADKYRCNFYPGGHKFD
                     LEMQKDAFEWFDRHLKP"
     gene            complement(4233..5519)
                     /locus_tag="VFB80_01815"
     CDS             complement(4233..5519)
                     /locus_tag="VFB80_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN32517.1"
                     /translation="MQRRTFLAASSAALAGLTQLGRAQDDPARFPRPAALPFRDEKSD
                     LQITAIRAVNLEPLRPLPKYQPAAGYWNTTDVEIANPLSIYPRFKPRRSLFYADDLGP
                     LTVAVETNKGITGYGYGGPGAAFVAEKHLPKLLVGEDPFQVERLWDIMWRGTLYYGRK
                     GAVVHGISAVDNALWDIVGKALQKPVYELLGAGQASRERVPCYCTGNNIEQAVEFGFQ
                     KLKLAIPHGPADGEAGLKANEALVERARKLLGDGGEVMLDCWMAFTEEYTEQLAARLA
                     PYRVYWMEECLMPDDYAGMGRLNAKIRSTRMVTGEHEATRWGFELLAHFKAASIWQPD
                     MHWCGGLTELRWIDALARREKIPVIPHGGWRGGSAHFVFANDNTPWCEMFLPAPGGPQ
                     EAYQQFEEEHQITRGPEGIYTRPPARPGFGFELKPA"
     gene            complement(5530..>7161)
                     /locus_tag="VFB80_01820"
     CDS             complement(5530..>7161)
                     /locus_tag="VFB80_01820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN32518.1"
                     /translation="LEVANEFAHGGFNHAILKTPRGQAELLALAKKTAPDLLVATSGM
                     GSGAVPQEVAAASDFLLIHFNSTKLEDIPTRIAALKKYGKPIVCNEDDKVGAAGAKAA
                     ELSVAAGASWGFMHSAVNQYFPKLSFSGPADDPVVYAALKRLTTKSDAFFPPPDAEGG
                     WRTLADAASIRRVAGMDKERLDEAFAFIQGSTKNGGLLVVRRGWLVYEDYFGLGHREA
                     TPNLASCGKSFTSIAVGILLAERPERFPDGLDQRVFTPDFFPPEAFPLSDPRKKDIRL
                     GQLLAFTAGIRGNNPSYVRGKPTTIDPVGPDGWQAKVEAFAFGKEDGKTGQTPFSTRA
                     LWCDPGEGYSYASSSIHLASVMLRHVTGTELQDYLQQRVAGPLGWGRWGFGYKDHPEV
                     AHTPGGGGIALRATDMLRFGYLLLNEGRWNGRQIVPAEFVRHCSRTSPYNPHFPYSLQ
                     FNVNTAAGAPELPRDAFWKSGSGGHAFYVVPSLDLVVWKLGGRDQQYSPANTGLPASP
                     APAEKVAARRDWKQTVDDQAALRKTLELVIKSIQP"
BASE COUNT         1142 a         2457 c         2354 g         1208 t
ORIGIN      
        1 ttaagggagt aaattggaca acagaagcag tttcgaccca cccaaccggg ggcctgataa
       61 ttgggtccat tgccgatcct gggaatcacg aatttggcgc gtgactgcgc agagccgccc
      121 gcaccagctt ggtcacttcg acaaccgtga ccggcgccag ggagagcagc gcgacgagca
      181 accaatcgcc ggacgtaaga tcggcatcca ctttgaagac cttgcgggca aacggcagcg
      241 tgacgacggc cagctgcaag aggccactga cggcgatcgc cccgagcaga tacgggttgg
      301 aaaacgggcc caattccggc agcgttcgtt gctcgctgcg gcaggcgaat gcaaagaaca
      361 gttgcccgaa agcgagaatg cagaaggcca cggtgcgggc ctcgtcgaac cgctccgtgc
      421 ggccgccata gaccagccag aagccgacgg tcgtcgccgc ggccaccaag aggccgtgcg
      481 tgacgatcag ccggccgcgc cgccgcgtga ggactggctc gtggggaggc cgcggcggcc
      541 gctgcatcac gtcgcggtcc ggccgctcca tcgccagggc cagggccggc aggccgtcgg
      601 tgaccaggtt gatccacagg atctggatcg cggccagcgg cgtcttccag cccaccagcg
      661 cggcgaagaa catgaacagc acctcgccgg cgttgcacga gagcaagtaa tgcacgatgt
      721 tccggacgtt gtcgaagatg cagcgaccct cctcgaccgc actcacgatc gagcggaagt
      781 tgtcgtccgt gagcaccatg tcggaggcct ccttggtcac gtccgacccc gtgataccca
      841 tcgcgatgcc gatttcggcc gccttcaccg ccggcgcgtc gttcacgccg tcgccggtca
      901 tcgccacgac ttcgcctcgg cgctgccagg ccttcacgat ccgcagcttg tgctcggccg
      961 aaacgcgggc atagacgctg gtccgatgga cgccgttggt cagcgtttcg tcgctcgtcg
     1021 cttgcagctc ctggcccgcg acgaccgtgt ccccttcatt caggatgccc aggtcgcggg
     1081 cgatggcctg cgcggtcgcc ggatggtccc ccgtgatcat gatcggccgg atgccggcgc
     1141 tcctacacgc cttcaccgcc acgcgagcct cgtcccgcgg tggatcgagc atgcccgcca
     1201 gccccgcgag caccagctcc ttttcggcga gctctctctc gcccggctgc cgctggcgat
     1261 acgccagggc cagtacccgc agggcgttgt cggccaactg ggcattcttg cgcaggacct
     1321 cctgccgccg ccgctcgtcg agcggctgct cctggccgcc gcgcagctcg cggctgcaca
     1381 tcgccaggac gacttcgggc gcacccttgg tacagatcag ctcgctgccg tccgactcac
     1441 gcacgatgac cgacatcgcc ttgcggttgg aatcgaacgg aatctcgctg gcgacctgaa
     1501 agtcgtcgcg cgcgaaaggc aggccggctt tgcccgcagc gacgagcaaa gcgccttcgg
     1561 tcggatcgcc gattacctgc caggactggt tctccttttc gctgcgctgg agccgagcgc
     1621 cgttgcagcg aacgccgacg gacagcagca ttcgcagacc gggttgatcg gctgctttga
     1681 tcgattcgcc tgcgccggcg cggcggaact ctccctcggg cttgtagccg ctgccggtga
     1741 cctcgaattc ttcgtcgcca gcgaccagcc ttcgcacggt catctcgttg cgggtcaacg
     1801 tgcccgtctt gtcggtgcag atcacggtga ccgagccgag cgtctccacg ctgggcagct
     1861 tgcggaccag ggcattgcgg gcggccatcc gctgcaggcc cagcgccagc gcgagcgtta
     1921 cgaccgccgg cagcccttcg ggcaccgcgg ctaccgccag gctcaccgag accaggaaga
     1981 cttgcagcaa ctcgccgccg cgaaccagtt gcaacacgaa cacgaccgcc accagcgcca
     2041 ggcacaccac caccagcacg cggcccagtt cggccagccg ccgctgcagc ggagtcggct
     2101 ccggcggctg ccgggcgagc agccccgcga tgttgcccag ctcggtttgc atgcccgtcg
     2161 ccacgaccag cgcggccgcc ttgcctttgg tgacgacggt gccggaatag acgctgttgc
     2221 tgcgctcggc cagcggcgtc tgttccggca atgtcacgcc cgggtctttt tcgaccggcg
     2281 acgactcgcc ggtcagcgct gcctcctgcg tttgcaggct ggcggccgaa atcagccggg
     2341 cgtcggcggg aacgtagtcg cccgcctcca gctcgatgta gtcgccagga agaagctcgg
     2401 ccgcgggaat ctgctgggct ttgcccccgc ggacgacctt ggcgttgggc gaggagagct
     2461 tccgcaaagc tgccagcgcc tgctcggcct tctcctcctg gatgaagccg acgatcccgt
     2521 tgagcagcac gatcgccagg atggcgattg cgtccgccac ttcacccatg gccgcagcga
     2581 tgatcgccgc gacgatcaga atccagatca cgatctcctt gaattggccc aggaaccgct
     2641 tccacatggg aaccggcggg gcctgttcca gttgattcgc accgtaccgc gaccgcagcg
     2701 ccgagacttg cgacggatcg agtcccctcg cgatatcgga acccaaagca gccgccagct
     2761 ggcctggttc gatgcgatgc gcgggcaccg gcgctcgatc cccagaagct tgttccggct
     2821 gcttcctggc atcattcatc tgatggatca ggcccagagc ggctcgagtt gcgctacggt
     2881 ttcaagtgcc ggtcgaacca ctcgaaggcg tccttctgca tctccaagtc gaacttgtgg
     2941 ccgcccggat agaaattgca gcgatacttg tcggccgcgc cgccgcgctc aaatgtctcc
     3001 cgcagcattg catccgcccg gcgcatttcc ggcacggtgt agagcgcgtc ctccgtgcaa
     3061 ttgaggacca tcgtggccgc cggcgctcgc agggcgagga tttccggaaa gtccaggtcg
     3121 cgcggcagca gcggcacgta ggccatccag gtgtgcgtaa aacacttgtc gagcaggaat
     3181 tctcgccagg tcgtcatgaa gccgacggcg acggcgcagc ggatgcgatc gtcgagcccg
     3241 cccaggaaca ccgtccgcat gccgccgccc gacaagccgg cgcacccgac ccgcttcgca
     3301 tcgacttccg gccgggcgca cagcacgtcg agcgcccgct gatcttcgac cacatacacg
     3361 ccgggccagg tcgtccccgc ggaaagcagg ctcttttcca tgatgtgctc gtgggcggcc
     3421 gcaaacgcgt tgtagcgttt gattccctcc gcgtcgtccg gctgcgggtc gatgccgttt
     3481 tgccgcagcc gctcgggcac ggcgtccacc cgcacgcggc ggctggcaaa cgggaacgtg
     3541 tcatggacga gcaccgcgta gccgcgtttg gcgatctcgt tcgcccaggc gactccgccg
     3601 taatagcgct gctgatggtc ggcgacgacc tcgcccggct tgtcatcgat gcgggcgatt
     3661 ttgccccagc ccaggaactt gtttccgccg tgatcgtgca gggcgagaat gccgggcaac
     3721 gggcccttag cgccggccgg tttcaagaag accgcctcgg ttcgcggacc gccgggcagc
     3781 tgccacgaca accgctcgat gtgcaggcca tcaaattcgt gcgtggattc cacgcggacc
     3841 tccggcctgc cgccgagatc gaccggcgcc atgcactccc agagtcgctc gcgtcccttg
     3901 gcccgccact cctccagcgt tttccatttg ccggttcgaa acgagagccg cgccggtccg
     3961 tcgcccagca ccgtttgcga cagccacggc ccgtacggcc cgaccatgtt caggcgcggc
     4021 gcactcggct gttgacccac ggcgctgcca attgccgcga ccagtattcc caacatcccg
     4081 agcaggtgga tcgcagattt catgagcttg actcccgttt gtgtccgccg cccgcacgca
     4141 ggccattgtg ctgggccgtg ccaggggatg caatttgggc cggtctgcta cggcgtggac
     4201 tcgctcgagg cagtcgccgg caaattgcgg cctcaggccg gtttgagctc gaagccaaaa
     4261 ccgggcctgg cgggcgggcg ggtgtagatg ccctcggggc cgcgggtgat ctggtgctct
     4321 tcctcgaact gctggtaagc ctcctggggc ccgccagggg ctggcaggaa catctcgcac
     4381 cagggcgtat tgtcgttggc gaagacgaaa tgggccgagc cgccgcgcca gccgccgtgc
     4441 gggatgacgg ggattttttc gcggcgggcc agggcgtcga tccagcgcag ctcggtcagc
     4501 ccgccgcacc agtgcatgtc gggctgccag atgctggcgg ccttaaagtg ggccaggagc
     4561 tcgaagcccc accgcgtcgc ctcgtgctcg ccggtgacca tccgcgtcga acgaatcttg
     4621 gcgttcaggc ggcccatgcc cgcgtagtcg tcgggcatca ggcactcctc catccagtaa
     4681 acgcggtagg gcgcgagccg cgcggcgagc tgctcggtgt actcctccgt gaaggccatc
     4741 cagcagtcga gcatcacctc gccgccgtcg ccgagcagct ttcgcgcacg ctcgacgagc
     4801 gcctcgttgg ctttcaggcc cgcttcgccg tcggccgggc cgtgcggaat ggccagcttg
     4861 agcttctgga acccaaactc cacggcctgc tcgatgttgt tgccggtgca gtaacaaggg
     4921 acgcgctcgc ggctcgcctg gccggcgccg agcagctcgt acaccggctt ctgcagcgcc
     4981 ttgccgacga tgtcccacag ggcgttatcg acggcgctga tcccgtgcac gaccgcgcct
     5041 ttgcggccgt agtagagcgt gccgcgccac atgatgtccc acagccgctc gacctgaaac
     5101 ggatcttcgc cgacgagcag cttgggcaga tgcttctcgg cgacgaaggc cgcgcccgga
     5161 ccgccgtagc cgtagcccgt gatgcccttg ttcgtttcga ccgcgacggt gagcgggccc
     5221 aggtcgtcgg catagaacag cgagcgccgc ggcttgaacc gcggatagat cgagagcggg
     5281 ttggcgatct cgacgtcggt cgtgttccaa tagccggccg ccggctgata cttgggcagc
     5341 ggccgcagcg gctccaggtt caccgcgcgg atggccgtga tctgcagatc gctcttttcg
     5401 tcgcggaagg gcagcgccgc gggccgcgga aagcgagccg ggtcgtcctg agcccggccg
     5461 agctgtgtga gtccggccag tgccgcgcta ctggcagcca agaaggtccg gcgctgcatc
     5521 gggggctcct tacggctgaa tggacttgat caccagttcc agcgtcttgc gcagcgcggc
     5581 ctggtcgtct acggtctgct tccagtcccg ccgcgcggcg accttttccg ccggggccgg
     5641 cgaggccggc aagccggtgt tggccggcga gtattgctgg tcgcggccac cgagcttcca
     5701 cacgaccagg tcgagcgacg gcacgacgta gaaggcgtgg ccgccggaac cgctcttcca
     5761 gaaggcgtcg cgcggcagct cgggagcgcc agccgccgtg tttacattga actggaggct
     5821 gtacggaaag tgtgggttgt agggcgacgt gcggctgcag tggcggacga actcggcggg
     5881 gacgatctgc cggccgttcc agcgcccttc attgagcagc agatagccga atcgcagcat
     5941 gtcggtcgcc cgcagcgcga tgccgccgcc ccccggcgtg tgcgccacct cgggatggtc
     6001 cttgtagcca aaaccccagc gcccccagcc cagcggccca gccacgcgct gctgaaggta
     6061 gtcttgcagc tccgtgcccg tgacgtgccg gagcatgacc gacgccaggt ggatcgacga
     6121 cgaggcgtag gaatagcctt cgcccggatc gcaccacagc gcccgcgtgc tgaacggcgt
     6181 ctggcccgtt ttgccgtcct ctttgccgaa ggcgaacgcc tcgactttcg cctgccagcc
     6241 atcgggcccg acgggatcga tggtggtggg cttgccgcgg acgtacgacg ggttgttgcc
     6301 gcggatgccg gcggtgaagg ccagcaattg gccgaggcgg atgtctttct tgcgcggatc
     6361 gctgagcggg aacgcctccg gcgggaaaaa gtcgggcgtg aatacgcgct ggtcgagccc
     6421 gtcggggaac cgctccggcc gctcggcgag caggatgccc acggcgatgc tcgtaaaact
     6481 cttgccgcac gaggcgaggt tcggcgtcgc ctcgcggtgg cccaggccaa agtagtcctc
     6541 atagaccagc cagccgcggc gcacgacgag caggccgccg tttttcgtgc tgccctggat
     6601 gaaggcaaag gcctcgtcga gccgctcttt gtccatgccg gcgacgcggc ggattgaagc
     6661 ggcgtccgcc agcgtccgcc agccgccctc ggcgtccggc ggcggaaaga acgcgtccga
     6721 cttcgtcgtc agccgcttta gcgcggcata gaccacggga tcgtcggccg ggccgctgaa
     6781 cgagagcttg gggaaatact gattgaccgc gctgtgcatg aagccccacg acgcgccggc
     6841 ggcgacgctc agctcggcgg ctttcgcgcc cgccgcgccc accttgtcgt cctcgttgca
     6901 aacgatcggc ttgccgtact tcttcagcgc ggcgatgcgg gtggggatgt cttccagctt
     6961 cgtggagttg aagtggatca gcaggaagtc gctggccgcc gcgacctcct gcggcaccgc
     7021 gccgctcccc atgccgctgg tcgcaacgag caggtccggc gcggtcttct tggccagcgc
     7081 gagcaactcg gcctggccgc gcggtgtctt cagaatggcg tggttgaaac cgccatgggc
     7141 gaactcgttg gcgacttcca g
//
LOCUS       DASRJB010000066        18430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3689016, whole genome shotgun sequence.
ACCESSION   DASRJB010000066 DASRJB010000000
VERSION     DASRJB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 18430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3689016"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..7497
                     /locus_tag="VFB80_01825"
     CDS             22..7497
                     /locus_tag="VFB80_01825"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZN32519.1"
                     /translation="MPFLYLRALDAPLGTLALGLAVSFAPAVALAVSLANALATRLLP
                     PRVLPKMAFDGGIPSDASTAVVVPALLTSVEDATVLARRLEIHYLANPDASLRFALLT
                     DLTDADEEERPDDREIVAQAVSRVRALNRRYGKDGHRPFHLLHRARRWNPAEGCWMGW
                     ERKRGKLMEFNRLLAGAADTTYAMQEGDLASLKPTGQHGSVQFVMTLDADTELPFGAA
                     RKLIGTLAHPLNRPHFDPHRESITAGYAVLQPRVSVHLGSANRTWFSRLYANHPGIDP
                     YTTAASDVYQDLFGEGSFTGKGIYDLRAFERSLAGRFPENHILSHDLIEGCHARVALV
                     SDIEVIDGFPARYDADARRQHRWVRGDWQILPWLFPWAPTASGWGRNRLSLLSRWKIL
                     DNLRRSLLGPAVLLLLFCGWWFWPALASLTTGAGLLVVAFPLLTQIGLALRGWPREGS
                     WWLHFQRVASELGRSLALAVLSLAFLPHQAVLMLDAIGRTLVRLLITRRKMLEWETAA
                     AAERRLSKSRWSTLWHLWYVPLFALAALLAGPSARAWALPIAALWLAAPLLAHWISLP
                     VAARKGSLTDEQRSWLRRAARRTWAFFETYVGAEDHWLPPDNLQEYPQEKLAHRVSPT
                     NEGLLLASALVARDFGYLGLSAAADLWERSWHQWLQLDRLRGHFFNWYDTLTLQPLFP
                     RYVSTVDSGNLAACLLTVREGIDELRQAPILGGELWRGLQDTATMAREACEALHPRGA
                     RLVDSPLNELGRAIDAIAARGGPPEGPLAWHTLLKSLQQDAAALRTSLEDFRASRQFP
                     PTDADPKVECLLRWIHEAHRDCRTLFPWTDVAQPALPPSSAGQTQQSWQRLLKLCQER
                     SSLAGLIDLPAAVGPHLADIRHCAAAELHGESLSAVKRRVEELAAAIDAGAQAALALS
                     QRLERLAAQMEQAALEMDFRFLYNPGRRLFSIGFNLEDEKLDRSHYDMLCSEARLTSY
                     LAIAKGDVEPKHWFQLGRQLTQTAGQAVLLSWGGTMFEYLMPPLFQRQYAQSLLSDTC
                     RGAVARQREYGRQCGVPWGVSESAFGALAVNSDYHYRSFGVPGLGLKRGLGEDLVVAP
                     YATMLALEVAPPAAIENLQRLEREGALGPWGLYDAVDYTPQRLPAGKRSMVVRCFMAH
                     HQGMSLLAMANLLLDGCVRRRFLGHPLARSTDLLLQERVPAAAPLLEPHADEAAAVEM
                     PAPTDELVSRRLVGVETATPRTHLMGNGQCSVMVTNSGGGYMLWRDIAVTRWRPDTTC
                     DGWGPFIYLRDVRSDQVWSATYQPTCATPDSYEVIYSIDKAEYLRRDGDLDTLLEVTV
                     SPENNAEVRQLKLTNHGSTPRQIEITSYAEVALCTPAADVAHPAFQKLFVETEYIAEE
                     TALLARRRPRDARQQPLWAVHVISGGDGGSIQYETSRQAFLGRGRSPRRPAALDPEVR
                     LSGQVGAVLDPVFSLRTTVTIRSHESATIGLTTALATSRDEALVLADQYHDARGVQRA
                     FELSWAYHQVQLRHLKLSPAKAHLFQQIAAAIIYPDPTRRGAKELLLANRLGQAGLWR
                     HGISGDLPIVLIHVTQPEHVGQVRDLLSAHAYWTSHGLGVDVVILNDYPGSYLDALQE
                     QLVALLSEALRVPNQPGGLFLLRAAQLPAEDKALLEAAAAVVLHGERGTIAKQWEAVR
                     EAAVPPPPLLSAGARKRQAAALAREVQDVRPGAQPDGRHYEFWNGTGGFTAEGREYRV
                     LLAAGNSTPLPWSNVIANPVFGTLVTESGGGYTWFANSRENKLTTWSNDPISDSLSEA
                     LYLRDETTGQIWSPLTGPARAAGDYWIHHGQGYSRFIHQAAGIDHEVEISIASGDPVK
                     FIRVRLANGGSASRELSLTYFVEWVLGVCREQTQLHVQTEIDAATGALLARNPYHPEL
                     GGQTALLHVLADNRTCTGDRTEFIGRNGDLRCPQALLRAKLSGACGAGLDPCGAVQTR
                     LRLAAGERAEVVFLLGAGENREQAQQLLAKYASPNAVEQACRQTRQQWDEILGAVQVK
                     TPNRALDLLVNRWLLYQTLSCRVWGRSAFYQASGAYGFRDQLQDCLALVYSRPDAVRE
                     HLLRAAARQFEEGDVQHWWHPPAGRGTRTRFSDDLLWLPFAAAHYVRTTGDASVLDEP
                     VPFLRSPPLAAHEQERYELPAVSDERGSLYEHCRRAIAKGFTTGPHGLPLIGCGDWND
                     GFNRVGEAGRGESVWVGWFLLVLLREFVPLMRTRGDGRLASEWETQADALRHALEQTA
                     WDGRWYRRAYFDDGTPLGSVQNDECRIDSIAQSWAVMAGAPQQRTDQALEAVMEQLVR
                     RDEKLVLLFTPPFDRTVLDPGYIKGYLPGIRENGGQYTHPAAWLIHALTQMGRIDDAL
                     AIFDLISPIHHAAAANAAKYQVEPYVVAADVYGAAPHVGRGGWTWYTGSASWLYRVAV
                     ESLLGLELRGNRLVLRPHLPSDWPGFELTLRRQGTTWKFVVSRNAAAPAVAEVELVDD
                     GRTHVVQVASG"
     gene            complement(7715..8833)
                     /locus_tag="VFB80_01830"
     CDS             complement(7715..8833)
                     /locus_tag="VFB80_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32520.1"
                     /translation="MLTIRGLSLTNLPRVALAGWRPLPRPLHVLICVADHFEPQWQRP
                     PASVAHDRAARWRNEYPRLAGRYKDSRGQSPQHTFFFPAEEYQPDHVEALAELCRLGL
                     GDVEVHLHHDNDTSDRLRQTLGEFTSALHQEHGLLARDAAGGITYGFIHGNWALDNSR
                     PDGRWCGVNDELTVLRQTGCYADFTMPSAPAACQTTTINSIYYATDDPLRPKSHDRGV
                     AARVGVRAPDGSLLMIQGPLVLDWRQRKHGMLPRIENGDLTALRPPSLDRLWSWLAAG
                     VYVAGREDWRFIKLHTHGAQEANSLMLLGEPMERFHSDLARFAAEHDWFHFYYVTARQ
                     MAELVHAAEQGRSGSPAEALTQQTEAKLPADLGQPCPS"
     gene            complement(8854..9999)
                     /locus_tag="VFB80_01835"
     CDS             complement(8854..9999)
                     /locus_tag="VFB80_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN32521.1"
                     /translation="MLWGSLGAVVYAYAIYPLLIYLLARAWGRQILAPQLNEDELPPV
                     SLLIAALNEEKWIADRVLNALHQDYPAGRLRVVVASDGSTDRTCQIVRDLADRYPGRV
                     VLFDFPVRRGKATVLNSALPKLSTEFVVLSDANTFFERSAVRNLVRWFRNPEVAAVCG
                     KLNLVDAATGRNVDGVYWRYENFLKGREALLGALLGANGAVYAIRRRDYVAIPGDTII
                     DDFVIPLQIKLRTRGRIVYDAAAVATEETPPHVSDEFRRRARIGAGGFQSAVRLWPLV
                     LPTIGWTSLAFVSHKLLRWCAPLFLLLALAANVLLLYRPEYQLLFAMQAGFYALAAVG
                     ALVPGNSAAIRGLRLTTLFASMNAALAVGFWRWLAGWQQGTWQRTAR"
     gene            complement(9881..10660)
                     /locus_tag="VFB80_01840"
     CDS             complement(9881..10660)
                     /locus_tag="VFB80_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZN32522.1"
                     /translation="MKLLELLAADLRAKAQWCYGNTRWRDLLKTLLTDGTAAMVWYRL
                     MQAAGRWRLAPLEMVCNKLNAVCCNCIIGRGADFGPGFVLIHSTGVVINGRVRAGSHV
                     YLEHQVTIGAEQRLSPVLGDRVFIGAGAKIVGPVTIGDDCRIGANAVVVRDIPSGATA
                     VGIPARVVRMYGKPPSLQATGGEAQSLLGTVATDSTDSGCAPHSLDADWHGFTADDCP
                     GDGALGLAGGRSIRLRDLPVADLPAGAGVGATDPGAAAKRG"
     gene            complement(10657..11883)
                     /locus_tag="VFB80_01845"
     CDS             complement(10657..11883)
                     /locus_tag="VFB80_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN32523.1"
                     /translation="MTTALATPILTQQAVADSDDGLRGRDIVCFSHDWAGDPLSKTHL
                     MRLLARHNRVLWVNSIGYRMPSANRADLGRIWKKLAAARQPVREVEPNIFVLNPLAIP
                     AYGSSVARRLNQWWLRVQIRGAMRRLDFRRPINWTFNPVAAVVAGMLGEEHLIYQCVD
                     EYSALAGVTPRAIRDLEARLLKEADLVIVSAQRLLQSKRCINPHTVMVRHGVDFELFR
                     RALEPQTIVPAEIARLPRPVLGFFGLLSEDWIDLELLVKLAQRYASGSLVLLGLVRMD
                     LSRLALPNVHVLGRQPYESLPAWCKGFDVGLIPFVQSELTLNANPLKAREYLAAGLPV
                     VSTDLPEVAVLPVCRIGRSHEGFLEQVEAALQQSGPRPERSEQMRSESWQARLEEIEA
                     HWRCRQGNAARRSRSP"
     gene            complement(11880..13091)
                     /locus_tag="VFB80_01850"
     CDS             complement(11880..13091)
                     /locus_tag="VFB80_01850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN32524.1"
                     /translation="MRILAITNLYPNPQQPHRAPYNRHQFRMLGELHPLRVIAPIAWT
                     DEWSARRRGLPALPSRRLQHDGLTVDYPRYWFTPKILRGLYGRFYLASIRATFRRVRD
                     EFQPEIIFAPWAYPDGHAAVQLARLSGLPVVVQVHGSDIRELDRFAARVPGTIAAVRD
                     ADGVIAVSQDLAERVIGLGAAPRRVRVVIDGVDKRTFHPGPKAEAQRRVGLSPAAKHL
                     LFVGNLVPVKGLDVLLEACRRLPGPRSQWQLHVVGAGPLDESLRSTAESLGLADSVSF
                     HGPIAHAELPDWFRAADLFVLASRSEGVPNVLLEAAACGLPFVASRVGGIPEIASIGA
                     SRLVPPEQPAVLAEAISGMLADPGPAGDGPRDRRDAVAEIAQFLESFVRPARPAPPAQ
                     TQSHPLSTTRA"
     gene            complement(13088..14278)
                     /locus_tag="VFB80_01855"
     CDS             complement(13088..14278)
                     /locus_tag="VFB80_01855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN32525.1"
                     /translation="MLTHSLPTAEQSTAVPSAPLHVVHGVLSLDCGGLERLVVNLVRE
                     GRRRQQRVSVVCIERPGRLSPAVEAAGAEVLSLDKPPGRSAAAVRQAAQLLQRLQPDV
                     IHTHQIGALWYLGQAARPQRIPVLHTEHSDHVAHARGWLAKLKIRWQWRTTARYARRF
                     CCVSDDIARSVRRYGTVPRKKVEVVPNGIDLAGYDSAAGSAEIRAELGLPAGTLVIGT
                     VGRLVEVKRQDLLIASFAQLRQRGRHPQTRLLIVGDGPERGRLEALARRLGVHEQTVF
                     AGYQAQPERMLRAMDLFVLTSRHEGMPLALLEAWAAGLAVVSSAVGSIPQTVMHGISG
                     MLFPSGDVAALTDTLERLLDAPWIISQLGRRGRARVESRYSLERMAAAYRQHYEALVA
                     KVQP"
     gene            complement(14313..15068)
                     /locus_tag="VFB80_01860"
     CDS             complement(14313..15068)
                     /locus_tag="VFB80_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZN32526.1"
                     /translation="MSLLTRLKRGEGPIWSRLKPFARQVLAFHLPVNGLTRPAWRGLY
                     AVHVGLREGWIWARRFFWHEPLFRSQCAAVGPGLWMEELPYIQGQGRITLGRHVRLSG
                     RPEIAFSNRHADNPELTIGDETFLGHLCNLRIARSVRIGSHCLIAGGVTIADFDGHPL
                     DAAARRAGATLTAGDIRPVEIGNDVWIGQSAVILKGVRIGDRAVVGAQAVVTRNVPAD
                     CVVAGNPARVVRNLEQRVQAERPAVQRELVAAE"
     gene            complement(15065..15967)
                     /locus_tag="VFB80_01865"
     CDS             complement(15065..15967)
                     /locus_tag="VFB80_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZN32527.1"
                     /translation="MKVSVIIPTFNSGRLVVQAIDSALAQTHAAAQIVVVDDGSTDDT
                     CDRIRPYLDRLEYVQQPNRRVAAARNAGLARVTGELIAFLDADDAWHPHKLARQVDVL
                     AQRPEIGVLATGLTDWPGPLAAELPPAPGTIAAVRLVEMLLFNPLATSSVVIRRELLE
                     RVGRFDTELFGPEDYDLWLRCAQQSQVAILREPLTGYRDTCGSLGKQAETMRQGLLRI
                     HAKLDAAGVWPNPWLRRKCRAHLDYTTGYMFFAGGKARQATRLLLQSLAGYPWPLSPP
                     DVRCHWARVRLLFRSLTSRKQVPS"
     gene            complement(15964..17172)
                     /locus_tag="VFB80_01870"
     CDS             complement(15964..17172)
                     /locus_tag="VFB80_01870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN32528.1"
                     /translation="MKTFRVAVAASGLGHVSRGIEAWAQDLGHALASRGVDTLLCKAA
                     GNPENEHERVIGCWTRESPQARRLVRRLPRFLGWRLGLGSGYGVEQTTFAWNLLGLLR
                     RERIDLLHVQDPQLALLVQRANRLGLVRTRSLLAHGTEEPLDFQRRITYLQHLAPWHL
                     EESRAAGVYKPTWTAIPNFIDTERFAPRDAKTESKALALRSELDIPPSALVVLSVSAI
                     KQHHKRIDYVLREFAAVRRKRPDLPVWLVIAGGWEQDTDELIRYGERCLGDRVRFLVR
                     FPRASMPELYRAADLFVLASLKEMMPIALLEATSSGLPSLVNQHPVMQWMIGAGGRAI
                     DMATEGSLAAELQPLLADDALRRQLGQQAREHCIANFSEDAVVEQILAYYEQILSPAP
                     QAAAALAAST"
     gene            complement(17169..18362)
                     /locus_tag="VFB80_01875"
     CDS             complement(17169..18362)
                     /locus_tag="VFB80_01875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN32529.1"
                     /translation="MTASPLTIGFDVRPGNSGPARYVAALARGLSDAGWNAVFLDSTE
                     PASAVPMEHTTNVPARRTAISFRRAVPAFARCWAGFHRESSRLASRLRSTSVDILHTQ
                     NTGCEEMPVAARWAGLPRVLGTFHVDSTYDLERVRSGLTHRVLEWYSNRSLHRAIAVS
                     EATKRDWMRRTGIAAKRVVTIHNGIDPDDFRRRQPIESARGQLGLPRDAVVLGGLGRL
                     DPAKGFGDLVEALALLHTEFPQLYVAIAGAGPLRESLAALAAERSVASRFILLGFQPD
                     VNLVLDACDVFVLPSLCEALPFALLEAMAHELPAVGTGVGGVPEVIVPGETGFVTPPR
                     DPQALAAALRPLVASAQLRERLGRAGRERVQRHFHERDMIRKTVDVYRELVRGQRMQR
                     TPAIA"
BASE COUNT         3097 a         6220 c         6150 g         2963 t
ORIGIN      
        1 gggacggcgg gcgccctcgc cgtgcccttc ctgtacctgc gcgcgctcga cgctccgctc
       61 gggaccctcg cgctcgggct ggcggtctcg ttcgcgcccg ccgtagcgct cgcggtctcg
      121 ctcgccaacg cgctcgcgac gcgcctcctc ccgccgcgcg tcctcccgaa gatggccttc
      181 gacggcggga tcccctccga cgcgagcacc gcggtcgtgg tcccggcgct cctgacgagc
      241 gtcgaggacg cgaccgtgct ggcccgccgc ctggagatcc actacctcgc caaccccgac
      301 gcctcgctcc gcttcgccct cctgaccgac ctcaccgacg ccgacgagga agagcggccc
      361 gacgaccgcg agatcgtcgc ccaggccgtc tcccgcgtgc gggcgctcaa ccgccgctac
      421 gggaaggacg gccaccgccc cttccacctg ctccatcgcg cgcggcgctg gaaccccgcc
      481 gagggctgct ggatgggttg ggagcggaag cgcggcaagc tgatggaatt caacaggctg
      541 ctggccggcg ccgccgacac cacgtatgcc atgcaggagg gggacctcgc cagcctgaag
      601 ccgaccggac agcacggctc cgtgcaattc gtcatgacgc tcgacgccga caccgagttg
      661 ccgttcggcg ctgcccgcaa gctgatcggc acgctggccc atccgctcaa ccggccgcat
      721 ttcgacccgc atcgcgaatc tattaccgcc ggctacgccg tgctccagcc gcgggtcagc
      781 gtccacctgg gcagcgcgaa ccgcacgtgg ttttcgcggc tatacgccaa tcatccgggc
      841 atcgatcctt acacgacggc ggcgtcggac gtttaccagg atttgttcgg cgaggggagc
      901 ttcactggca agggaatcta cgacctgcgg gcgttcgagc ggtcgctcgc cggccgtttt
      961 ccggaaaatc acattctcag ccacgacctg atcgagggct gccacgcccg cgtggcgctg
     1021 gtcagcgaca tcgaagtgat cgacggcttc cccgcccgct acgacgccga tgcccggcgg
     1081 cagcatcgct gggtccgcgg cgactggcag atcctgccgt ggctgtttcc ctgggctccc
     1141 accgccagcg gctgggggcg taaccggctg tcgctgctca gccggtggaa gattctcgac
     1201 aacttgcggc gcagcttgct cgggcccgca gtgctgctcc tgctcttttg tggctggtgg
     1261 ttctggccgg cgctggcctc gctgacgacg ggagcgggtc tgctggtcgt ggcgtttccg
     1321 ctgctgacgc agattggcct ggcgctgcgc ggctggccgc gcgaaggatc gtggtggctg
     1381 catttccagc gcgtggcatc cgagttgggc cgctcgctgg ccctggcggt actctcgctg
     1441 gcgttcttgc cgcaccaggc cgtcctgatg ctcgatgcca tcggacgaac gctcgtgcgg
     1501 cttctgatca cacgccgcaa gatgctggag tgggaaaccg cggctgcggc cgagcggcga
     1561 ctatccaaga gccggtggtc gacgctatgg catctttggt acgtgcccct cttcgcgctg
     1621 gcagcgctat tggccggtcc gtccgcccgc gcttgggccc tgccgattgc agcgctgtgg
     1681 ctggccgcgc cgcttttggc gcactggatc agcttgccag tcgccgcgcg gaagggcagc
     1741 ctgacggacg agcaacggtc gtggctgcgg cgggcagcgc gccgcacgtg ggcctttttc
     1801 gagacctacg tcggcgcgga ggaccactgg ctgcctcccg acaatctcca ggagtacccg
     1861 caggaaaagc tcgcccaccg ggtttcgccg acgaacgaag ggctgctgct ggcttcggcg
     1921 ctggttgccc gcgacttcgg ctacctcggc ctctcggccg ccgcggacct ttgggagcgc
     1981 agctggcacc agtggcttca gctggaccgc ctccgcggtc actttttcaa ttggtacgac
     2041 acgctcacgc tccagccgct ctttccgcgg tacgtttcga cggtcgatag cggaaacctg
     2101 gccgcgtgcc tattgaccgt gcgcgagggc atcgacgagt tgcggcaagc gccgatcctc
     2161 ggcggggagt tgtggcgcgg tttgcaagat acggcaacga tggcccgcga ggcctgtgaa
     2221 gccctgcatc cccgcggggc gcggctggtc gattcaccgc tcaacgaact cggccgggcg
     2281 atcgacgcca tcgccgcccg tggtggaccg cccgagggcc cgctggcgtg gcatacgctg
     2341 ctgaagagct tacagcagga cgcggcggcc ctgcggacca gcctggagga ctttcgcgca
     2401 tcgcggcaat tcccgcccac cgacgccgat ccgaaagttg aatgtctgct gcggtggatc
     2461 cacgaggctc accgcgactg ccggacgctg ttcccctgga cggatgtggc ccagccggcg
     2521 cttccgccgt cgagtgccgg ccagacacaa cagtcgtggc agcgcctgct caaattgtgc
     2581 caggagcggt cgagcctggc gggcctaatc gatttgcccg cagccgtcgg gcctcatctg
     2641 gcggacatcc gccactgtgc agcggcggag ctgcacggcg agagcctgtc cgcggtgaag
     2701 cggcgcgtcg aggagctggc agccgcgatc gacgcgggcg cccaggctgc actcgcgctc
     2761 agccagcgcc tggaacggct cgcggcccag atggaacagg ccgcgctgga gatggatttc
     2821 cggttcctct acaaccccgg ccgccgactg ttctcgatcg gcttcaatct ggaggacgaa
     2881 aagctcgacc gctcgcacta cgacatgctc tgctccgagg cccgactgac gagctacctg
     2941 gcgatcgcga agggggacgt tgagccgaag cactggtttc aactaggccg ccagctgacg
     3001 cagactgccg ggcaggccgt gctgctctcc tggggcggca cgatgttcga atacctcatg
     3061 ccgccgctct tccagcggca atacgcgcag tcgctgctga gcgacacgtg ccgcggggcc
     3121 gtcgcccggc agcgcgaata cgggcggcag tgcggagtgc cgtggggcgt gtcggagtcg
     3181 gcgttcgggg ccttggcggt gaactcggac tatcactacc gctcattcgg cgtgccgggg
     3241 ctaggcttga agcgcgggtt gggggaggac ctggtggtgg cgccttatgc gaccatgctc
     3301 gcgctggaag tcgcgccgcc ggccgcaata gagaatctgc agcgcctgga gcgtgaaggg
     3361 gccctcggac cttggggact gtatgacgcg gtcgattaca cgccgcagcg gctgccggcc
     3421 ggcaagcggt cgatggtcgt ccgctgcttc atggctcatc accagggcat gagcctgctg
     3481 gcgatggcca acctgctctt ggacggttgc gttcggcggc ggtttctcgg ccatccgctg
     3541 gcccgttcga ccgacttgct ccttcaggag cgcgtgccgg ccgccgcccc gctgctcgag
     3601 ccgcacgccg acgaggcggc cgccgtcgag atgcccgcgc cgaccgacga gttggtcagc
     3661 cggcggctcg tcggcgtcga gacggcgacg ccgcggaccc acttgatggg caacgggcaa
     3721 tgctcggtga tggtcaccaa cagcggcggc ggctacatgc tgtggcggga catcgcggtg
     3781 acgcgttggc ggcccgatac gacgtgcgac ggctgggggc cgttcatcta cctccgcgac
     3841 gtccgctcgg atcaggtctg gtccgcgact taccagccga cgtgcgcaac gccggactcc
     3901 tacgaggtga tctactcaat cgacaaggcc gagtacctcc gccgcgacgg cgatctcgac
     3961 acgctcctgg aggtaacggt ctcgcccgag aacaacgccg aggtgcggca gctcaagctc
     4021 accaaccacg gctcaacgcc aaggcaaatc gaaatcacaa gctacgccga agtggcgctg
     4081 tgcactcccg cggccgacgt cgcgcatccc gcctttcaaa agctgttcgt cgagaccgag
     4141 tacatcgcgg aagagacagc cctcttggcc cgccggcggc cgcgcgacgc ccggcaacag
     4201 ccgctatggg cagtgcacgt catttccggc ggcgacggcg gctcgatcca gtacgagacg
     4261 agccggcagg cgtttttagg ccgcggacgc tcgccccgcc gtccggcggc gctggatccc
     4321 gaggtccgcc tgagcgggca ggtcggcgcg gtgctcgatc cggtcttctc gctgcggacg
     4381 acggtaacga ttcgctcgca cgagtcggcg acgatcggct tgaccacggc cctggccacc
     4441 agccgtgacg aggccctcgt gctggccgat caatatcacg atgcgcgcgg cgtgcagcgg
     4501 gcgttcgagt tgtcttgggc ctatcaccag gtgcagctgc gacacctgaa gctctcgccg
     4561 gccaaggccc acctctttca gcagatcgcc gcagcgatca tctatcccga cccgactcgc
     4621 cgcggcgcga aagagttgct cctggccaat cggctcggcc aggctgggtt gtggcggcac
     4681 ggaatctcgg gagacttgcc catcgtgctg attcacgtca cgcagccaga gcacgtcggc
     4741 caagtccgcg atttgctttc ggcgcacgcc tattggacgt cgcacgggct gggcgtcgat
     4801 gtggtgattc tcaacgacta tcccggcagc tatctcgatg cgcttcagga acagctggtc
     4861 gcgctgctga gcgaggcgct gcgcgtgccc aatcagcccg gcggcctgtt cctgctgcgt
     4921 gccgcgcagc tgccggccga ggacaaggcg ctattggagg cggccgctgc ggtcgtgctc
     4981 cacggcgagc gaggcacgat cgccaagcag tgggaggccg tgcgcgaagc agccgtgccg
     5041 cccccgccgc tgctctcggc cggcgcgcgc aagcgccagg ctgccgcgct ggctcgcgag
     5101 gtccaggacg ttcgtcccgg cgctcagccc gacggccggc attatgaatt ctggaacggc
     5161 accggtggct ttacggcgga agggcgcgag taccgcgtgt tgcttgcggc gggaaactcg
     5221 acgccgctgc cctggtcaaa cgtcatcgcc aatcccgtgt ttggcacgct cgtcacggag
     5281 tcgggcgggg gttacacgtg gttcgccaac agccgcgaga acaagctcac tacgtggtcg
     5341 aacgacccga taagcgattc gctctcggaa gccctttatc ttcgcgacga aacgacggga
     5401 caaatctggt cgccgctcac cgggccagcc cgcgctgcgg gagactactg gattcaccac
     5461 ggccagggct attcgaggtt catccaccag gccgccggca tcgatcacga agtcgagatc
     5521 tcgattgcct ctggcgaccc ggtcaagttc atccgcgtgc gtttggccaa cggtggttcg
     5581 gccagccgcg aactgtcgct gacctatttc gtcgagtggg tactgggagt ctgccgcgag
     5641 cagacgcagc tgcacgtaca aaccgagatc gacgccgcga ccggcgctct attggcccgc
     5701 aatccctatc atcccgaact ggggggccag acggcgctac tgcacgtact ggcggacaac
     5761 cgcacctgca cgggcgaccg cacggagttc attggccgca acggcgatct gcgttgcccg
     5821 caggcactcc tgcgggcgaa gctctcgggc gcgtgcgggg cgggactcga tccgtgcggg
     5881 gccgtgcaga ccaggcttcg gctcgcggcg ggcgagcgcg cggaagtcgt ctttctgctc
     5941 ggcgcgggcg agaatcgcga gcaggcgcag caattgctgg cgaagtacgc ctcgccgaat
     6001 gccgtcgagc aggcctgccg ccagacgagg cagcagtggg acgaaatcct cggcgcggtg
     6061 caggtcaaga cgcccaaccg ggccctcgac ctgctcgtga accgctggct gctctatcaa
     6121 acgctgagct gccgggtgtg gggccgctcg gcgttctacc aggccagcgg cgcatatgga
     6181 tttcgcgacc agttgcagga ttgtctggcg ctggtctaca gccggccgga cgcggtccgc
     6241 gagcatttgc tgcgggcggc ggcgcggcag ttcgaagagg gggacgtaca gcactggtgg
     6301 catccgcccg ctggccgcgg cacgcggacg cgattctcgg acgacttgct ctggctcccc
     6361 ttcgcggcgg cacattacgt ccgcacgacg ggcgacgcga gcgtgctgga cgagcccgtg
     6421 cccttcctgc gctcgccgcc gctggccgcg cacgagcagg agcgatatga gttgccggcc
     6481 gtcagcgacg agcgcggctc gctctacgag cactgccgcc gggcgattgc caagggcttc
     6541 accaccgggc cgcacgggct gcccctcatc ggctgcggcg actggaacga cggcttcaac
     6601 cgggttggcg aggccggccg aggcgagagc gtgtgggtcg gctggttcct gctggtgctg
     6661 ctccgcgagt tcgtgcccct gatgcggacc cgcggcgacg ggcggctggc cagtgaatgg
     6721 gaaacacagg ccgacgcgct gcggcatgcc ctggagcaga cggcgtggga tggccgctgg
     6781 tatcgccggg cgtatttcga cgacggcacc cccctcggct ccgtacagaa cgatgagtgc
     6841 cggatcgact cgattgcgca gtcctgggcc gtcatggccg gcgcgccgca gcagcgaacc
     6901 gaccaggcgt tagaagccgt gatggagcag ctcgtccgcc gcgatgaaaa actggtgctg
     6961 cttttcacgc ctcccttcga tcgcacggtc cttgatccgg gctacatcaa gggctatctg
     7021 ccggggattc gcgagaacgg cggccagtac acgcaccccg cggcctggct gattcacgcg
     7081 ctgacgcaaa tgggccgcat cgatgacgca ctggcgatct ttgaccttat cagccccatt
     7141 caccacgccg cagcggcgaa tgccgccaag taccaggttg agccctacgt cgtcgccgcc
     7201 gacgtctatg gcgctgcgcc ccacgtcggc cgcggtgggt ggacctggta caccggctcg
     7261 gcttcgtggc tgtatcgcgt ggcggtggag tccttacttg gtctggagct gcgcggcaat
     7321 cgcttggtcc tacggccgca cctgcccagc gattggccag gtttcgagct cactttgcgc
     7381 cgccaaggca ccacctggaa gttcgtcgtc agtcgcaacg ccgccgcacc ggctgtcgcc
     7441 gaagtcgaac tcgtggacga cgggcggacg cacgtcgtac aggtcgccag tggatagtcg
     7501 gccgccgcac tcctggcatt cccacatcga ctcggccgag cgcagccagc aagcaatcca
     7561 gccggcgatc catagcccgc ccgtgagcag gctcgctagt gcatgaaaac ggtgttcggg
     7621 caaggccaag cggcgcgaaa acagcaccag gcggccgcaa tggtggcagc ggccggaagc
     7681 tatctcgcgg cagtggatgg cggggagcat gttctcagct ggggcaaggc tggcccaggt
     7741 cggcagggag ttttgcctcg gtctgctgtg tcaaggcctc ggcgggcgaa cccgagcgtc
     7801 cttgctcagc ggcatgcacc agttcggcca tctgccgcgc ggtgacgtag taaaagtgaa
     7861 accagtcatg ctcggcggca aagcgcgcca ggtcggagtg aaagcgttcc atcggctcgc
     7921 cgagcagcat cagcgagttg gcctcttgtg caccgtgcgt atgcagtttg atgaaccgcc
     7981 aatcctcccg gccggcgacg tacacgccgg ccgccagcca actccacagc cggtcgagcg
     8041 agggcggacg cagggcagtg aggtcgccat tctcgattcg gggcagcatg ccgtgcttgc
     8101 gctggcgcca gtcaagcacg agcggccctt gaatcatgag gagcgagcca tcgggcgcgc
     8161 ggacgcccac ccgcgccgcc acaccccggt cgtgtgactt cggccgcagc gggtcgtcag
     8221 tcgcgtaata gatgctgttg atggtggtcg tctggcaggc cgccggcgct gaaggcatcg
     8281 tgaaatcggc gtagcagccg gtctggcgca gcacggtcag ctcgtcgtta acaccgcacc
     8341 agcggccgtc gggccggctg ttatcgagtg cccaatttcc gtgaatgaaa ccataagtaa
     8401 tgcctcccgc cgcatcgcgg gcgagcagcc cgtgttcctg atgaagtgcg gacgtgaatt
     8461 cgccgagcgt ctgccgcagg cgatcggacg tgtcgttgtc gtggtgcaga tgcacctcga
     8521 cgtcgcccag gccgagccgg cacaactcgg ccagggcctc gacgtggtcc ggctgatatt
     8581 cctccgccgg aaagaaaaag gtgtgctggg gcgactggcc acggctgtcc ttgtagcggc
     8641 ccgccagccg tgggtattca ttccgccatc gcgcggcccg gtcgtgggcc acactggcag
     8701 gcggacgctg ccactgcggc tcaaagtggt cggcgacgca gatcaggaca tggagagggc
     8761 gcggcagcgg cctccatcca gcgagggcga cgcgcggcag gtttgtgagc gacaacccgc
     8821 gaatggtcag catggtcgca cactcgcggc tgcttagcgg gccgtccgct gccaggttcc
     8881 ctgctgccag ccggcaagcc accgccagaa gccgacggcc agtgcagcat tcattgaagc
     8941 gaatagcgtc gtcaacctca gcccacgaat ggcggcgctg ttgcccggca ccagtgctcc
     9001 gacggcggcc aaggcgtaga aacccgcctg catcgcaaat aacagctgat attccggccg
     9061 gtagagcagc aacacgttcg ccgccaatgc cagcagcagg aacagcgggg cgcaccaccg
     9121 cagcagctta tgcgagacga aagccagcga cgtccaacct atcgtgggta gcactagcgg
     9181 ccacagtcgc acggcgctct gaaaaccccc cgcgccgatt cgtgcccggc ggcggaattc
     9241 gtcggacacg tgaggcggcg tttcctcggt cgcgacggct gcggcgtcat agacgatccg
     9301 cccccgtgtt cgcagcttga tttgcagcgg aatcacgaag tcgtcgatga tcgtatcgcc
     9361 ggggatggcc acgtaatcgc ggcggcgaat ggcatagacg gctccattcg cgccgagcag
     9421 ggcaccgagc agggcctcgc gccccttgag gaaattctcg taccgccagt acacaccgtc
     9481 cacgttgcgg ccggtcgccg catcgacgag attcagcttt ccacagacgg ccgccacctc
     9541 cggattacga aaccagcgga caaggtttct gacagcagat cgctcgaaga acgtgttcgc
     9601 atcggacaaa acgacgaatt ccgtggacaa cttcggcagc gcggagttga gcacagtcgc
     9661 cttgccgcgg cggacgggaa aatcgaacag caccacgcgg ccaggatagc ggtcggccag
     9721 gtcccgcacg atctggcacg tgcggtcggt gctgccgtct gacgccacca cgacccgcaa
     9781 tcggcccgcg gggtaatcct ggtgcaatgc gttgagcacg cggtcggcga tccatttctc
     9841 ctcgttcagt gccgcgatca gcaagctgac cggcggcagc tcatcctcgt ttagctgcgg
     9901 cgccaggatc tgtcgccccc acgcccgcgc cagcaggtag atcagcaacg ggtaaatcgc
     9961 gtaggcgtat actacggccc ccagcgagcc ccagagcacc gtcgccaggg caatcgtcag
    10021 cggtgaagcc atgccaatcg gcgtccaacg agtgcggcgc gcagcccgag tctgtcgaat
    10081 cggttgctac ggtgccgagc aaactttgtg cctcgccgcc ggtcgcttgc agcgacggcg
    10141 gctttccata catccgaacc acgcgagctg ggatccccac tgccgtcgca ccggacggaa
    10201 tgtcgcggac cacgacggca ttggcgccaa tccggcagtc gtcgccgatc gtgaccgggc
    10261 cgacgatttt cgcgcctgcg ccgatgaata cgcgatctcc cagcacggga gatagccgct
    10321 gctcggcccc gatcgtcacc tgatgctcca gataaacgtg gctgccggct cgcacacggc
    10381 cgttgatgac aacgcccgtc gagtggatca gcacgaagcc gggtccaaaa tccgccccgc
    10441 ggccgatgat gcagttgcag cagacagcgt tgagcttgtt gcagaccatt tccagcggcg
    10501 ctagtcgcca gcgccccgcc gcctgcatca ggcgatacca aaccatggcc gccgtgccgt
    10561 cggtcagcag cgtcttgagc aggtcccgcc agcgcgtatt gccatagcac cactgggctt
    10621 tggcccgcag atctgccgcc agcaattcca gcagcttcat ggcgagcggc tcctgcgggc
    10681 agcgttgcct tggcggcaac gccaatgcgc ttcaatctct tccaagcggg cctgccagct
    10741 ctcgctccgc atttgctcac tacgctcggg ccgtggtccg gactgctgga gtgcggcttc
    10801 gacttgctcc aggaacccct cgtgcgagcg gccgatccgg catacgggca gcaccgcgac
    10861 ctccggcaga tcggtcgaca ccacgggcaa gcccgcggcc agatattcgc gagctttcag
    10921 cgggttggcg ttgagcgtca attcgctctg tacgaacgga atcagcccga cgtcaaaacc
    10981 cttgcaccag gccggcagcg actcgtacgg ctggcggcca agcacgtgca cgttgggcag
    11041 cgccaggcgc gacaaatcca ttcgcaccag acccagcagg acgagcgagc cgctcgcata
    11101 ccgctgggcc agcttgacca gcaactccaa atcgatccag tcctccgaca aaagaccgaa
    11161 gaaacccagc acgggccgcg gcagccgggc gatttcagcc ggaacgatcg tctgcggctc
    11221 cagagcgcgg cgaaacaatt cgaaatcgac gccgtggcgc accatcaccg tgtgcgggtt
    11281 gatgcagcgt ttggactgga gcagccgctg ggcggaaaca atgacgagat ccgcctcttt
    11341 gagcaaccgt gcttccaggt cgcggatggc ccgcggcgtg acgcccgcca gcgccgagta
    11401 ttcatcgacg cactgataga tgaggtgttc ctcgccgagc atccccgcga cgactgcagc
    11461 cacggggttg aacgtccagt tgatcgggcg gcgaaaatcc agccggcgca tagcgccgcg
    11521 aatctgcacg cgcaaccacc actggttcag gcggcgggcc acgctggaac cataggcggg
    11581 gatcgccagc gggttgagca cgaagatgtt cggctcgacc tcgcgcaccg gctgccgggc
    11641 agccgccagc tttttccaga tgcggccgag atcggcccta ttggcgctcg gcatccggta
    11701 accgattgag ttgacccaca gcacccggtt atgcctggcc agcagccgca tcaaatgagt
    11761 cttggaaagc ggatcgccgg cccaatcgtg actgaagcag acgatatcgc gtccccgcag
    11821 gccgtcgtcc gaatcggcaa cagcctgttg cgtcaagatc ggagttgcca gggcggtcgt
    11881 catgctcttg tcgtggaaag gggatgggac tgcgtctgcg caggaggcgc cggtcgcgcg
    11941 gggcggacga aggactccag gaactgggcg atttcggcca cggcgtcgcg tcggtcgcgc
    12001 ggtccgtcgc cagccggtcc ggggtctgcc agcattccgg atatcgcctc ggccaggacc
    12061 gccggctgct cgggaggaac gagccggctg gcgccgatgc tggcgatctc cggaatgccg
    12121 ccgacgcggc tagccacgaa cggcaggccg caggccgcgg cttccaggag cacgttgggc
    12181 actccctccg agcggctggc gagcacgaac aggtcggcgg cgcggaacca atcgggcaat
    12241 tcggcgtggg cgatcgggcc atgaaacgag acgctgtctg ccagccccag cgactcggcc
    12301 gtgctccgga gcgactcgtc gagcggcccc gcgccgacca cgtgcagctg ccactggctg
    12361 cgaggcccag gcaggcgccg gcacgcttcc agcaagacgt cgagtccctt gacgggaacg
    12421 aggttcccca caaagagcag gtgtttggcc gcgggcgaaa ggccgactcg ccgctgggct
    12481 tcggccttcg gccccggatg aaacgtccgc ttgtccacgc cgtcgatgac gacgcggacc
    12541 cgccgcggcg ctgcgcccag cccgatgacc ctctcggcca aatcctgaga cacagcgatc
    12601 acgccgtcgg catcgcggac tgcggcgatc gtacctggga cgcgcgcggc aaagcgatcg
    12661 agctcacgaa tatcagagcc gtgtacttgc acgaccaccg gcagcccgga cagccgagcc
    12721 agttgcacgg cggcgtggcc atcgggatag gcccagggcg cgaagatgat ctcgggctgg
    12781 aactcgtccc gcacgcggcg gaaggtcgcg cggattgagg ccaggtaaaa ccggccatac
    12841 aagccgcgga gaatcttggg cgtgaaccag tagcgcgggt aatcgacggt cagcccgtcg
    12901 tgctgaaggc ggcgagacgg cagcgccggc aaaccccgcc gccgggccga ccattcgtcg
    12961 gtccaggcga tgggcgcgat gacccgcagc ggatgcagtt cccctagcat tcggaactga
    13021 tgccggttgt agggcgcgcg gtgcggctgt tgcgggttgg gatataggtt ggtgattgcg
    13081 agaattctca cggctgaacc ttggcgacga gcgcctcgta atgctggcga taagcggcgg
    13141 ccattcgctc cagcgaatag cgggattcga ctcgagcgcg gccgcggcgg ccgagctggc
    13201 tgatgatcca cggagcgtcg agcagccgct caagcgtgtc ggtgagcgcc gccacgtcgc
    13261 cgctgggaaa cagcattccg ctaatgccgt gcataactgt ctggggaatc gatcccacgg
    13321 ccgatgacac gaccgccagt cctgccgccc aggcttccaa gagggccagc ggcatcccct
    13381 catgccggct agtaagtacg aacaggtcca tcgcccgcag catccgctcc ggctgagcct
    13441 ggtagcccgc gaacaccgtt tgctcgtgga cgccgaggcg gcgggccagg gcctccagcc
    13501 ggccacgttc cggtccgtcg ccaacgatga gcagccgcgt ctgcggatga cgaccgcgct
    13561 ggcgcagttg agcgaaggag gcaatgagca gatcctggcg tttgacctcc accaaccggc
    13621 cgacggtgcc tatgacgagc gttcccgccg gcaagcccag ctcggcgcgg atctcggccg
    13681 aaccggcagc ggagtcatag ccagcgaggt cgatgccgtt ggggacgact tcgactttct
    13741 ttcgcggtac cgtgccgtag cggcgcacag accgggcgat gtcgtccgag acgcagcaaa
    13801 accgccgggc ataccgggcc gtggtccgcc attgccagcg gatcttgagc ttggccagcc
    13861 aaccccgcgc gtgggcaacg tggtcggagt gttcggtgtg gagcacaggg atccgctgcg
    13921 gccgcgccgc ctggcccagg taccaaagcg ccccgatctg atgcgtgtgt atgacgtcag
    13981 gctgcaagcg ctggagtagc tgcgcggcct gccgaacggc ggcggcgctg cgtccgggcg
    14041 gcttgtccag gctcagtact tcggcgcccg cagcttcgac tgccggactc agtcgccccg
    14101 gccgctcaat gcacacgacc gagacgcgct gttggcgccg ccggccttcg cgaaccagat
    14161 tcaccaccag gcgttctagc ccgccgcaat ccagtgacag gacgccgtga acgacatgca
    14221 agggcgcgga gggaacagcg gtcgattgtt ccgcggtcgg cagcgaatgg gtcaacatgt
    14281 cgcgaggccc tcccaaggtt tgtgcctgcc cctcattcgg ccgcgaccag ctcccgttgc
    14341 acggccgggc gttccgcctg cacacgctgc tccagattac gaacgactcg cgccgggttg
    14401 ccggcgacga cgcaatcagc cggcacgttg cgagtcacaa cggcctgcgc accgacaacg
    14461 gcccggtcgc caatccgaac gcccttgagg atcaccgcgc tttgtccgat ccagacgtcg
    14521 ttgccgattt ccacgggccg gatatcgccg gccgtcaatg tcgccccggc ccggcgcgct
    14581 gctgcgtcga gcggatgtcc atcgaaatcg gcgatcgtta cgcctcccgc aatcaggcag
    14641 tgcgagccga tgcggaccga gcgggcaatc cgcaagttgc agaggtgccc gagaaacgtc
    14701 tcgtcaccga tcgtcagctc cggattgtcg gcgtgtcggt tgctgaacgc gatttccggc
    14761 cggccagaga gcctcacgtg gcggccgagc gtgattcggc cttgcccctg aatatagggc
    14821 agctcctcca tccacagccc cggtcccacg gcggcgcatt ggctgcgaaa tagcggctcg
    14881 tgccagaaaa atcggcgggc ccaaatccag ccctcgcgga gtccgacgtg tacggcgtat
    14941 aggccccgcc acgcgggccg cgtcaggccg ttgaccggca ggtgaaacgc caacacctgc
    15001 cgcgcgaacg gcttcaggcg gctccagatt gggccttcgc cccgtttcag acgagttagc
    15061 aggctcatga cggcacttgc ttccgggagg tgagcgagcg aaacagcagc cgcacccgcg
    15121 cccaatggca gcggacatcc ggcggcgaca ggggccaggg atagccggcg agcgactgca
    15181 agagcagccg cgtggcctgc cgcgccttgc cgccggcaaa gaacatgtat ccggtcgtgt
    15241 agtcgagatg ggcgcgacat ttccgccgca gccaagggtt gggccagact ccggccgcgt
    15301 cgagtttggc gtgaatccgc aacaggccct gccgcatcgt ctcggcctgc ttgccgagac
    15361 tgccgcacgt gtcgcgatag ccggtcagcg gctcgcgcag aatcgcgacc tgcgactgct
    15421 gcgcacagcg gagccagagg tcgtaatcct cggggccgaa cagctcggtg tcgaatcggc
    15481 ctacgcgctc cagcagctcc cggcgaatga caaccgacga cgtggccagt ggattgaaga
    15541 gcagcatttc caccagcctc acggccgcga tcgtgcccgg tgcgggcggc agttcggcag
    15601 ccaacgggcc cggccagtcc gtcaggcccg tcgccaacac gccgatctcc ggccgctgcg
    15661 cgagcacatc cacttgccgc gccagcttgt gcgggtgcca ggcgtcgtcg gcgtcgagaa
    15721 acgcgatcag ctcgccggta acccgcgcca gtcccgcgtt gcgggccgcc gcaacgcggc
    15781 gattgggctg ctgcacgtac tcgaggcgat cgaggtaagg ccgtatccga tcgcaggtat
    15841 cgtcggtcga gccgtcatcg accaccacga tctgcgcggc ggcatgcgtc tgcgccaagg
    15901 cgctgtcgat cgcctggacg accaggcggc cgctgttgaa ggtcggaatg atcacgctga
    15961 ctttcatgtg ctcgccgcca gggccgcagc cgcctgcggc gccggtgaga ggatttgctc
    16021 gtaataggcc aggatctgct cgacgactgc gtcctcgctg aagttggcga tgcagtgctc
    16081 gcgagcttgc tgtccgagtt ggcggcggag cgcgtcgtcg gccagtagcg gctgcagttc
    16141 ggcggccagg ctgccctccg tggccatgtc gatcgcgcgg ccgcccgcgc cgatcatcca
    16201 ctgcatgacg gggtgctggt tcaccaggga cggcaagccg ctcgaggtgg cttccaacag
    16261 agcgatcggc atcatctcct tgaggctggc gagcacgaac aggtccgcgg ctcgatagag
    16321 ctccggcatc gaagcgcgcg ggaaccgcac caggaaccgg actcgatcgc caaggcatcg
    16381 ttcgccgtag cggatcaact catcggtgtc ttgctcccaa ccgccggcga tgacaagcca
    16441 caccggcaaa tccggccgct ttctgcgcac cgccgcgaat tcgcgaagca cgtagtcaat
    16501 gcgcttgtga tgctgcttga tcgccgaaac acttagcacc acgagtgccg acggtggaat
    16561 gtccaactcg ctccgaagtg ccagcgcctt gctctctgtt ttcgcatcgc ggggagcgaa
    16621 tcgctcggtg tcgatgaaat tgggaatcgc cgtccaggtc ggcttataga cgcccgccgc
    16681 ccgcgactct tccaggtgcc acggcgccaa gtgttggagg taagtgatgc gccgctgaaa
    16741 atcgagcggc tcttcggtcc cgtgagccag tagcgaccgc gtgcgcacga ggcccagccg
    16801 attggcacgc tgaacgagca gggcgagttg cgggtcctgc acgtgcaaca ggtcgattcg
    16861 ttcgcgccgc agcagtccga gcaaattcca ggcaaacgtc gtctgctcga cgccgtagcc
    16921 gctgcccagc cctagccgcc agcccaagaa tcgcggcagc cgccggacca ggcgccgcgc
    16981 ctgcggcgat tcgcgtgtcc agcagccgat cactcgctcg tgctcgttct ctgggtttcc
    17041 ggcggccttg cagaggagcg tgtccacgcc tcggctggcg agcgcatggc ccaagtcctg
    17101 agcccatgct tcgatccccc gggagacatg tcccaggccc gaagcggcaa cggcgacgcg
    17161 aaaggtcttc atgcgattgc aggcgtcctt tgcatgcgct ggccgcgcac caactcgcgg
    17221 tagacgtcca ccgtcttgcg gatcatgtcg cgctcgtgaa agtgccgctg gactcgctcg
    17281 cggccggccc ggcccaggcg ctcgcggagc tgcgctgacg ccacgagtgg gcgcagggcc
    17341 gccgccaggg cctgcggatc tcgcggcgga gtcacgaatc ccgtctcgcc cggcacgatc
    17401 acctccggca ctccaccgac acccgtaccg actgccggca gttcgtgggc catcgcttcg
    17461 agcagggcaa acggcagcgc ctcgcatagc gacggcaaga cgaacacatc gcacgcgtcc
    17521 agcaccaggt ttacatcggg ctgaaagccg agcaggatga aacggctagc gacgctgcgt
    17581 tcagcggcca gcgcggccag cgattcgcgg agcggcccgg ccccggcgat ggccacatac
    17641 agctgcggaa actcggtgtg caggagcgcc agcgcctcta ccagatcgcc gaaccccttg
    17701 gccggatcga gccgtcccag accgccgagg acgaccgcgt cccgcggaag accgagctgc
    17761 ccacgcgccg attcgatcgg ttgccgtcgg cgaaagtcgt cgggatcgat tccgttgtga
    17821 attgttacga cgcgcttggc ggcgattccg gtccgccgca tccagtcgcg cttggtggct
    17881 tcgctgacgg cgatcgcccg gtgcaaactg cggttgctgt accactccag cacgcggtgc
    17941 gtcaggccgc tgcgaacgcg ctccaggtcg tacgtcgaat cgacgtggaa cgtgcccagc
    18001 acccgcggca agccggccca acgagcagcg accggcattt cctcgcaacc ggtgttttgc
    18061 gtgtgcagaa tatccaccga ggtcgagcgc aacctgctcg ccaggcggct ggattctcgg
    18121 tgaaagccgg cccagcaacg ggcaaaggct ggaacggcgc ggcggaacga gatcgcggtc
    18181 cgccgcgcag gcacgttggt ggtgtgctcc atcggcacag ccgaggcagg ctctgtcgaa
    18241 tccaggaaga ccgcattcca gccggcgtcg gagagtccgc gcgccagggc agctacatag
    18301 cgggctggcc ccgagttgcc gggccgcacg tcgaagccga tggtgagggg cgatgcggtc
    18361 atgatgctcg ctaagggacg gttgccagct cgggccgctg cgccggcgaa tgcgtgcggc
    18421 agcgggcgta
//
LOCUS       DASRJB010000067         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3408065, whole genome shotgun sequence.
ACCESSION   DASRJB010000067 DASRJB010000000
VERSION     DASRJB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3408065"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFB80_01880"
     CDS             <1..210
                     /locus_tag="VFB80_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32530.1"
                     /translation="ALALDGEPCGEQPLDLYNYPDVLASGEVELGRRSLTAGPHRLTV
                     TIDGANAAATRAYLVGIDYLRLVPR"
     gene            223..1848
                     /locus_tag="VFB80_01885"
     CDS             223..1848
                     /locus_tag="VFB80_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN32531.1"
                     /translation="MRPIAFAMLWILLAAAAATGQERDVRRASDEIDRLIDRGLAEAK
                     LTSAPPASDAEFLRRVTLDITGRVPAYEETVTFLESSDPAKRRLAIDRLLDSPAYGQH
                     FATIWNELITPRDKGSTKAVRDPFTPWLAEQFNRNRGWDAIVTELLTAAGKLRDNPQT
                     GFIAANCENFEPQPNLLADSTARLFLGIQLRCAECHDHPFAPWKQTDFWEMAAFFSHL
                     RKGLTSGKNPAGWTFTESPPEDEMNERFSVWHVAPGESGAALVVPQTGGKLAGQVVRA
                     RFLRAPQHFAGTQPPYRPQFAEWVVSRENPWFAANAANRMWAGLFGRGLVEPLDSFSA
                     DRAPAESAVLEFLARELAESGFDLKHLIRSITSSRAYRRTSQALSQNEADTDRFGRMA
                     VKPLRPEVLYDSLSVVLYPVLPKPGAKPSTAQPLAALPKTPREEFVRAFAMRPDETVG
                     SQVNTGVPQFLKLLNGDLLNRETPGLSRLLKGDPTPAEAIESIYLAALSRRPSAEDRL
                     WMLEYVEGAGDRRAAYQDVLWTLLNSGEFVLNH"
     gene            1882..3204
                     /locus_tag="VFB80_01890"
     CDS             1882..3204
                     /locus_tag="VFB80_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32532.1"
                     /translation="MSQIPNPQSPIPNSFSRRELLRLAAAGVGSASLSGWFGALAARA
                     AEPSNAKRRTRSCILLWMDGGPSHIETFDPKPDAPESIRGDLAAIATSVPGIQVGEKF
                     PQVAQLMQHGAILRGMSTREADHGRARIYMHTGYRPGAGGVTYPGLGSTVSAELGDAA
                     AELPNFVVTGSPLNKYDFLRDPGYRGPLHQPVVLNDLGRGLENSEPAVPPAEFDRRTA
                     LLERVEGAFARQYASPPAAAHQAGLASALKLIRSDKRGCFDLSQEPDSSRRKYGESDF
                     GRGCLLARRLIEAGVAFVEVYLSNWDSHFRDVAAQTRTLMSQVDSGLSSLVGDLHERG
                     LLDSTLVIWMGEFGRTPRVNTTGGRDHYAKAWSSVLFGGGIPGGQTVGATDAQGAEVI
                     DRPIAASDFMATICRILGIDFTKEIVAPGGRPIRIVEKDERLIRELLG"
     gene            complement(3460..4245)
                     /locus_tag="VFB80_01895"
     CDS             complement(3460..4245)
                     /locus_tag="VFB80_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HZN32533.1"
                     /translation="MLAHVHVMKTAGQTVREILRQSFLGRHCDLRAVGEVATAADLRW
                     ALRVYPGLKSIAGHQVRLYGDLDAVGPPLSYFTFLRDPVTRCVSHFQFGRRNGSQATL
                     EEFLTCHADHQTRHLAGTSDAQAAIELLEERRVFVGLMERFNESLVLLERWSGEALQL
                     PYRSQNIAASSELKRRLLADERALARIRECHQQDLELYQYVRDVIYPRQLQRFGSELA
                     AEVAELEAALPAPTILSFGRLLAKAKRDLLYKPLTRSPHRRAA"
     gene            complement(4345..5670)
                     /gene="nusA"
                     /locus_tag="VFB80_01900"
     CDS             complement(4345..5670)
                     /gene="nusA"
                     /locus_tag="VFB80_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZN32534.1"
                     /translation="MNPADMLRIVDSLHREKNIDKEIVFLAIEAALVTAAKKHYGELE
                     DIQVTINRETGEVTGLHNGLPMDQAEVSGRIGAQTAKQVIIQKIREAERDSLLGEYGE
                     QIGTMVNGVVTRNEGPATIVSLGTIEAILPRSEQIPGETHHPNERVRAIIFEVKPQGS
                     RVKVVLSRTRPQLIQRLFEQEIPEIADGVITINAVAREPGHRSKVAVSSSDQRVDCVG
                     ACVGVRGNRIKNIVDELAGERIDIVRYNDDAETLIRASLQPAEVDQVLLCDMIGRAIV
                     LVREDQLSLAIGRKGQNVRLASKLCGWDIEIMTNEELEQQIDRAVGGFMQIEGMNEEV
                     AQRLVEQGYLSYDDLSVIEPDALMEMGGLTEEQVAHIVEQADVLAQQAEKAADEERRR
                     KKQEAAAAEAAAAAGGAAAPATATPPAEAAAPPAPAAEPTASKDESQPA"
     gene            complement(5701..>6326)
                     /locus_tag="VFB80_01905"
     CDS             complement(5701..>6326)
                     /locus_tag="VFB80_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN32535.1"
                     /translation="IRLEGKDLAGVPPHRRNVALVFQNLALYEHLTARDNLAFGLHDK
                     GGQQVTETAKLLGIEHLLTRFPAELSGGEQQRVALGRAIVRQPAALLLDEPLSSLDGP
                     ARRDLRRELKRLQRQLGVPTIYVTHDQAEALLLGDRIAVIDKGRLQQMGPPEEIYQHP
                     ANRFVTEFFDLQEIHALAEKFRRSDRSTEPLTTDNSPAPAANHIPNP"
BASE COUNT         1015 a         2091 c         2091 g         1129 t
ORIGIN      
        1 gcactggcac tcgacggcga gccctgcggc gagcagccgc tcgacctcta caactatccc
       61 gacgtgctcg ccagcggcga ggtggagctc ggccggcgga gcctgactgc cggcccgcat
      121 cggctgaccg tgacgatcga cggcgccaac gccgccgcga caagagcgta cctggtcggt
      181 atcgattact tgcggctcgt tccgcgctag gaggctgcgc cgatgcggcc gattgccttc
      241 gcgatgctgt ggatcctcct ggcggcagcc gcggccaccg ggcaagagcg cgacgtgcgg
      301 cgcgccagcg acgaaatcga ccggctgatc gatcgcggcc tggccgaggc gaaactcacg
      361 tccgcaccgc cggccagcga cgctgaattc ctccggcgcg tcacgctcga catcaccggc
      421 cgcgtgccgg cctacgaaga aaccgtcaca tttctggaga gcagcgatcc cgccaagcgc
      481 cggctggcga tcgatcggct gctcgactcg ccggcctacg ggcaacactt cgccacaatc
      541 tggaacgagc tgatcacgcc tcgcgacaag ggcagcacca aggcagtccg cgatccattc
      601 acgccctggc tcgcggagca gttcaatcgc aatcgcggct gggacgcgat tgtcaccgag
      661 ctgctgaccg ccgcaggcaa gcttcgcgac aacccgcaga ccgggttcat cgcggccaac
      721 tgcgagaact tcgagccgca gccgaacctg ctggccgatt cgacggcccg gctgtttctg
      781 ggcattcagc tgcggtgcgc cgaatgccac gatcatccgt tcgcgccgtg gaagcagacg
      841 gacttctggg aaatggcggc gttcttcagc catctgcgaa aggggctcac cagcggcaag
      901 aatcccgccg gctggacgtt caccgagtcg ccgcccgagg acgagatgaa cgagaggttc
      961 agcgtctggc acgtcgcgcc gggcgagagc ggcgcggccc tcgttgttcc ccagaccgga
     1021 ggcaagctgg ctggccaggt cgtgcgggcc cgcttcctta gggcgccgca gcattttgcc
     1081 gggacgcagc cgccctatcg accgcaattc gccgagtggg tcgtcagccg cgaaaacccc
     1141 tggtttgcgg ccaacgccgc caaccgaatg tgggccgggc tctttggccg cggactcgtc
     1201 gagccgctcg attcgtttag tgccgatcgg gcgccggcgg aaagcgccgt gctcgaattt
     1261 ctggcgcggg agctggcgga gtccggattc gacctgaagc acctgattcg ctcgatcacg
     1321 agcagccggg cgtatcggcg cacaagccag gcgctgtcgc agaatgaggc tgacactgat
     1381 cggttcggcc gcatggcggt gaagccgctg cggccggagg tgctctacga ctcgctgtcg
     1441 gtggtgctct atcccgttct gcccaagccg ggggcgaagc cgagcacagc gcagccgctt
     1501 gcggccctgc cgaagacgcc tcgggaggag tttgtgcggg ccttcgcgat gcggcccgac
     1561 gagacggttg gcagccaggt gaacaccggc gtcccgcagt tcctgaagct gctcaacggc
     1621 gacctgctga atcgcgagac gcccggcctg tcgcggctgc tcaagggcga tcccacccca
     1681 gcagaggcga tcgagtcgat ctatctggcg gccctctcgc gtcggcctag cgcggaagat
     1741 cgcctctgga tgctggaata cgtcgagggc gccggcgacc ggcgggcggc ctatcaggac
     1801 gtcctctgga ccctgctcaa tagcggtgaa tttgtcctca atcactaggc ggagtgagtg
     1861 gtgagtggta gagcaagagt catgtcccaa atccccaatc cccaatcccc aatcccaaat
     1921 agtttctccc gccgcgagct gctgcggctg gcggcggccg gagtcggcag cgcgtcgctt
     1981 tcgggctggt tcggcgcgct ggcggcgcga gccgccgagc cgtccaatgc caaacggcgg
     2041 accagatcgt gcatcctgct ctggatggac ggcggtccga gccacatcga gacgttcgat
     2101 cccaagcccg acgcgcccga gagcatccgc ggcgatctgg ctgcgatcgc cacgtccgtg
     2161 cccggcattc aagtcggtga gaaattcccg caggtcgcac agctcatgca gcatggggcg
     2221 atcctccgcg ggatgagcac ccgcgaggcc gaccacggtc gggcccggat ctacatgcac
     2281 accggctacc ggccgggcgc gggcggcgtg acgtatccgg gcctgggctc taccgtctcg
     2341 gccgagctgg gcgatgccgc ggcggagctg ccgaacttcg tcgtcaccgg ctcgccgctg
     2401 aacaagtacg acttccttcg cgatcccgga tatcgcgggc cgctgcacca gcccgtcgtc
     2461 ttgaacgatc tgggccgtgg tctcgaaaac tccgagccgg ccgtaccgcc ggcggagttc
     2521 gaccgccgca cggcgctctt ggagcgagtc gaaggcgcct tcgcgcggca gtacgcttcg
     2581 ccgccggccg cggcgcacca ggccgggctg gccagcgctc tgaagctcat ccgctccgac
     2641 aagcgcggct gcttcgacct ctcgcaggag ccggacagct cgcgccgcaa atatggcgag
     2701 agcgattttg gccgcggctg cctgttggcc cgccggctga ttgaggcggg cgtggctttc
     2761 gtcgaagtct acctgtcgaa ctgggactcg cacttccgcg acgtggccgc ccagactcgc
     2821 acgctgatgt cgcaagtcga tagcgggttg tcgtcgctgg tcggcgacct gcacgagcgc
     2881 gggctgctcg attcgaccct cgtgatctgg atgggcgagt tcggccgcac gccgcgggtc
     2941 aatacgaccg gcggccgcga ccactatgcc aaggcctggt cgagcgtgct gttcggcggc
     3001 gggattcccg gtgggcaaac ggtcggcgcc accgacgctc aaggggccga agtcatcgac
     3061 aggccgatcg ccgccagcga cttcatggcc acgatctgcc gaattctggg catcgatttc
     3121 acgaaggaga tcgtcgcgcc gggcggccgc ccgatccgca tcgtggaaaa ggacgagcgg
     3181 ctgattcgcg agctgctcgg ctaggaactc tcagctgcgg ggcccctggt tgggcgggct
     3241 gcgccagccc ttcgaacgaa atctcggctg caacgtctgt ggcagcaatg atttacgccg
     3301 atcgcaacga aataccttaa gtagtactta ttagccttag agaacgccca gtttgtgcgg
     3361 tcccgcgctg gttctgttgc cgaaatggca ggcaggcgcg ccaatgggaa ctatactgca
     3421 aatagcgtac caatgttcag actgcggccg ggtttcgcct cacgccgcac gccgatgcgg
     3481 gctgcgggtc agtggcttgt agagcaggtc ccgcttcgcc ttggccagga gccggccgaa
     3541 cgaaaggatg gtcggggcgg gaagcgcggc ctcgagttcc gccacttcgg cggccagctc
     3601 gctgccgaat cgctggagct gccgcggata gatcacgtcg cggacgtact ggtaaagctc
     3661 cagatcttgc tgatggcatt cgcgaatacg cgccagcgcc cgttcgtctg ccaggagccg
     3721 ccgtttcagc tcgctgctgg cggcgatgtt ctgcgaacgg tagggcaact ggagtgcctc
     3781 gccgctccag cgctcgagca ggacgagcga ctcgttgaac cgctccatta gccccacgaa
     3841 aacgcggcgc tcctcgagca gctcgatcgc cgcttgggcg tcgctggtcc ccgccaggtg
     3901 ccgcgtttga tggtcggcgt ggcaggtgag gaattcctcg agcgtcgcct gcgaaccgtt
     3961 ccgccggccg aactggaagt gcgagacgca gcgcgtgacc gggtcgcgga ggaacgtgaa
     4021 atagctcagc ggcgggccga ctgcgtccag gtcgccatac aggcgaacct ggtgccccgc
     4081 gatgctcttc agccccggat agacgcgcag cgcccagcgc aaatccgccg cggtcgccac
     4141 ttcgcccacg gcccgcaagt cgcagtggcg gccgagaaac gactggcgca ggatttcccg
     4201 caccgtctgt ccggcggttt tcatcacgtg cacgtgcgcc agcatcgcga tcctcgtgcg
     4261 gccattatcg gctggaagca cgcagaatca tcagactttt tcgattcgtt gctggccgag
     4321 gccgccgctg cagctagcag ccagctaggc cggctgcgac tcgtccttgg aagcggtcgg
     4381 ttcggcggct ggcgcgggtg gtgcggcagc ctcggcgggc ggcgtcgcgg tggcgggcgc
     4441 agcggcgccc ccggctgcgg cggcggcttc ggcggcggcg gcttcctgct tcttgcggcg
     4501 gcgttcttcg tcggcggcct tttcggcttg ctgggccagc acgtcggcct gctcgacgat
     4561 gtgggcgacc tgctcctccg tgaggccgcc catttccatc agggcgtcgg gctcgatcac
     4621 cgagaggtcg tcgtacgaca gatacccctg ctcgaccagc cgctgggcga cctcctcgtt
     4681 catgccttcg atctgcatga agccgcctac ggcgcggtcg atctgctgct ccagctcctc
     4741 gttggtcatg atctcgatgt cccaaccgca gagcttgctg gccaggcgga cgttctgccc
     4801 cttgcggccg atggccaggg agagctggtc ctcacggacg agcacgatcg cccggccgat
     4861 catgtcgcag agcagcacct gatcgacctc ggccggctgc agcgaggccc ggatcagcgt
     4921 ctcggcgtcg tcgttgtagc ggacaatgtc gattcgctcg ccggccaact cgtccacgat
     4981 gttcttgatg cggttgccgc ggacgccgac gcaggccccc acacaatcga cccgctggtc
     5041 gctgctggac acggcgacct tgctgcggtg gccgggctcg cgggcgaccg cattgatcgt
     5101 gatcacgccg tcggcgattt cggggatttc ctgctcgaac agccgctgaa tgagctgcgg
     5161 ccgcgtgcgg ctaagcacga ccttcacacg gctgccctgc ggcttgactt cgaagataat
     5221 cgcccgcacg cgctcgttgg ggtggtgcgt ctcaccggga atctgttcgc tgcggggcag
     5281 gatcgcctcg atcgtgccga gcgagacgat cgtcgcggga ccctcgttcc gcgtgacgac
     5341 gccgttaacc atcgtgccga tctgctcgcc gtattccccc aggagcgaat cgcgctcggc
     5401 ctcgcggatt ttctggatga tgacctgttt ggcggtctga gccccaatcc ggccgctgac
     5461 ttcggcctgg tccatcggca ggccgttgtg caggccggtc acctcgcccg tctcgcggtt
     5521 gatcgtgacc tggatgtctt ccagctcgcc gtagtgcttc ttggcagcgg tgaccagggc
     5581 tgcctcgatg gccagaaaga cgatctcctt gtcgatgttc ttctcgcggt ggagcgaatc
     5641 gacgatgcgg agcatgtcag cggggttcat ggtcaggccc tcgggcaagt tgcgggcaaa
     5701 tcagggattc gggatatggt tagccgcggg tgcgggtgag ttgtcggtgg tcaggggttc
     5761 cgttgatcga tcgctgcgtc gaaatttctc tgcgagggcg tggatttctt gaagatcaaa
     5821 aaactctgtc acgaaacgat ttgcggggtg ctgataaatt tcttccggcg gacccatctg
     5881 ctgcaaccgt cctttatcga tcactgctat acgatcgccg agcagcaagg cttccgcctg
     5941 gtcatgggtg acgtaaatcg tcggcacgcc gagttgtctt tgcagacgct tcagctcccg
     6001 ccgcaggtcg cgccgcgccg gcccatcgag gctcgacagc ggctcgtcca gcagcaaagc
     6061 cgctggctgc ctgacgattg cccggcccaa ggccacccgc tgttgctctc caccggaaag
     6121 ctccgccgga aagcgcgtca gcaggtgctc gatccccagc agcttcgccg tttcggttac
     6181 ctgttgtcct cccttgtcgt gcaatccaaa cgccaaattg tcgcgagccg tcaggtgttc
     6241 gtacaacgcg aggttctgaa aaaccaaggc cacgttccgc cgatggggcg gaacgccggc
     6301 caggtccttc ccctcgagcc gtattg
//
LOCUS       DASRJB010000068         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_869720, whole genome shotgun sequence.
ACCESSION   DASRJB010000068 DASRJB010000000
VERSION     DASRJB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869720"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFB80_01910"
     CDS             <1..1135
                     /locus_tag="VFB80_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN32536.1"
                     /translation="MKYFFLSILSSALLLYGFAFLYGLGKTTLIAGTPAMPGIREALA
                     SPKLLAASPLAPLAGLALALIAAGLGFKLAIVPLQFYAPDVYQGTTNANAGLLAVAPK
                     IAGVAGFIRLVVVAMPGAADLAWQVALVLAIVTMTIGNVCALWQKNVRRLMAYSSIAH
                     GGYLLIGLAAAAAAASAGQPTIRGGVTSMLFYVLVYSLATMGTFAALSFLGSVRRDVS
                     GVDELAGLARSQPLVAGALAVFMFSLAGLPPLAGFWGKLNLFSAAVQLATTDLKGGVP
                     LCFAVLAVAGALNAAIAAAYYLRIVAVMYFQSAAQPAAAAGGRTAWLATVLCALLVVG
                     VGALPGRLMQAAVNSESVLRPGIQTVQQQPPAPQIAATAAAPQ"
     gene            1202..2632
                     /locus_tag="VFB80_01915"
     CDS             1202..2632
                     /locus_tag="VFB80_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN32537.1"
                     /translation="MPRPLSPGRLLLRTLEETATPLFVLDSQRRIVFASRSLGEWAGV
                     DAEKLVGLRCDYHTGGDVPLTSVAAALCPPPEAFTGTVASGSVGRPALANRPSEQRPA
                     RFVTLSGKSAAEGGLLLVVVQPPQELPAAETLAAPTPERLHALLADLRGQLGKRFSIG
                     QLIGESHVMHAVREQVRVAAETRARVLVVGPQGSGREHVARTIHHARQAPGPLVPIAC
                     ALADAEQMQRLLAGVLRQAEPGEQPAEALLLDVDRLSKEAQQELAGFLQFPHIELHTL
                     ATAGRPLSRLVTKGKFRADLALALGTLTIRLPGLARRRRDIPLLAQHFLEEANAAGGP
                     QRAGFLPAALERLLALPWPGNIDQLAAAVREACERAAGPRIAPADLPDWIEAAESAAQ
                     RAPRDDESIRLDELLEQIEREILERALRKARGNKSKAAELLGVNRNRLLRRLAQFGLI
                     EPAAAEEPVVFEPLPEQEPAPPSQKS"
     gene            complement(2620..3834)
                     /locus_tag="VFB80_01920"
     CDS             complement(2620..3834)
                     /locus_tag="VFB80_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HZN32538.1"
                     /translation="MAATSPLQFPQADTPGADMVFAPYEGKSPCGEFDNPFNNYYGDT
                     RTVRSLVLGRAWAEMEYLAWASKPTHLPALISTSPSGTDPADAGILGPNGSVLFGADT
                     TQNTLRSGARFNFGYWFTPQHIDGVETRLLWVGGERINFHAIGGDDLILATPFIDATT
                     DLPSAVLISFPGLLDGEARVRSNMQLFGLEVLGRHMLGCEACSRLDVIGGWRYLRLED
                     TVSADSIFLSLDDASGFDTDTIVARSDSFSSKNDFHGGEFGLVYRWWNCCWAVNLTGK
                     VALGGTRTIAQVRGTTTTISPTNDVTVTEGGVFAQPSNIGQYERKDFAAVGDVGVKLE
                     YAFTPQLRFTLGYNWMAWSSVARIGESIDTVVDPSQLAPNSNPAATRPGFAFRDNSFW
                     SHGLTLGGYYDF"
BASE COUNT          639 a         1454 c         1446 g          727 t
ORIGIN      
        1 gatgaagtac ttcttcctca gcatcctctc gtcggcgctg ctgctgtacg gattcgcgtt
       61 cctgtatggc ctggggaaga cgacgctgat cgccggcaca cccgcgatgc cgggcattcg
      121 cgaggcgctg gccagcccga agctgctcgc cgccagcccg ctcgcgccgt tggccgggct
      181 ggcgctggct ctcatcgccg caggcttggg attcaagctg gcgattgtcc cgctgcagtt
      241 ctacgcgccg gacgtttatc agggcacaac caacgccaac gcgggtctcc tggccgtcgc
      301 gccgaagatc gccggcgtcg ccggattcat ccgcctggtc gtcgtggcga tgccgggagc
      361 cgccgacctg gcgtggcaag tggcgctggt gctggcgatc gtcaccatga cgatcggcaa
      421 cgtctgcgcc ctgtggcaga aaaacgtccg ccgcctgatg gcctattcgt cgatcgccca
      481 cggcggctac ctgctgatcg gcctggcggc ggcggcagct gcggcatcgg ccgggcagcc
      541 gacgatccgc ggcggcgtca cgtcgatgct gttctacgtc ctggtctatt cgctggcgac
      601 gatgggcacg ttcgccgcat tgtcgttcct cggctcggta cgccgcgacg tgagcggcgt
      661 cgatgagctg gccggcctgg cccgctcgca gccgctggtc gccggggcgc tggccgtctt
      721 catgttctct ctggccgggc tgccgccgct ggccggcttt tggggaaagc tcaacttgtt
      781 cagtgctgca gtgcagctcg ccacgaccga tttgaagggc ggcgtgccgc tctgcttcgc
      841 ggtgctggca gtcgccggcg cgctcaatgc cgccatcgcc gccgcctatt acctgcggat
      901 cgtcgccgtc atgtacttcc agagcgcggc ccagccggcc gccgcggctg gtggccggac
      961 ggcctggctg gccaccgtgc tgtgcgccct gctcgttgtc ggcgtcggcg cacttcccgg
     1021 ccggctgatg caggccgccg tcaactccga gagcgtgctg cggccgggaa tccagaccgt
     1081 tcagcagcag ccgccggcac cacaaatcgc cgccacggct gccgcgccgc aatagcggcg
     1141 ggcgaagtat aatgacggct ggcccacgtt ttctcgcgtt ccagccgctg gccgttcgct
     1201 catgccacgc ccgctttctc ccggccgcct gttgttgcga acgctcgagg agacggcaac
     1261 ccccctcttt gtgctcgaca gtcaaaggcg gatcgtcttc gccagccgat cgctgggcga
     1321 gtgggccggc gtggatgcgg aaaagctcgt cggactgcgg tgcgattacc acaccggcgg
     1381 cgacgtgccg ctgacttcgg tcgccgctgc gctctgcccg ccgccggaag ccttcacggg
     1441 cactgtcgcc agcggatccg tgggcaggcc ggcgctggcc aatcgcccgt ctgagcagcg
     1501 gccggcgcga ttcgtgacgc tgagcggaaa gtccgccgcg gaaggcggat tgctgctggt
     1561 cgtcgtgcaa ccgccccagg agctgccggc ggccgagact ttggcagcgc cgacgcctga
     1621 gcgactgcac gccctgctgg cggatttgcg cgggcagctt ggcaagcgat tttctatcgg
     1681 ccagctcatc ggcgagagcc acgtgatgca cgcggtgcgg gagcaagtcc gcgtcgctgc
     1741 agaaacccgc gcccgcgtgc tggtcgtcgg cccgcaaggc agcggccgcg agcacgtcgc
     1801 ccggacgatc caccacgcgc gccaggcgcc gggtccactc gtgcccatcg cttgcgcgct
     1861 ggccgatgcc gagcagatgc agcggttgct cgccggcgtc ctccggcaag ccgagccggg
     1921 cgagcagccc gccgaggcgc tgctgctgga cgttgatcgg ctgagcaaag aggctcagca
     1981 ggaattggcg ggctttctgc agtttccaca tatcgagttg cacacgctgg ccactgcggg
     2041 ccggccgcta tcgcggctgg tgaccaaggg caagttccgc gcggacctgg ccctggccct
     2101 gggcacgctc acgatcaggc tgcccgggct ggcccgtcgc cgccgcgata ttccgctatt
     2161 ggcccaacac tttctcgagg aagccaacgc ggcgggcggg ccgcagcggg cgggcttcct
     2221 gccggcggcg ctggagcgcc tgctggcact gccctggccg ggcaacatcg accagctggc
     2281 cgccgcggtc cgcgaggcct gcgagcgagc ggccggcccg cggatcgccc cggccgattt
     2341 gcccgactgg atcgaggcgg ccgagagcgc ggcgcagcgc gccccgcgcg acgacgagtc
     2401 gattcggctg gacgaactgc tcgagcaaat cgaacgcgaa atcctggagc gggccttgcg
     2461 gaaggcccgc ggcaacaagt cgaaggcggc ggagctgctc ggtgtcaacc gcaaccggtt
     2521 gctgcgccgc ctggctcaat tcggcctcat cgagccggcc gccgccgaag aacccgtcgt
     2581 cttcgagccg ctgccggagc aagagcccgc gccgccgtct caaaaatcgt agtagccacc
     2641 cagcgtcagg ccgtgcgacc agaagctgtt gtcccggaaa gcgaaaccgg ggcgcgtggc
     2701 ggcggggttg ctattggggg cgagctgcga aggatcgacg accgtgtcga tcgactcgcc
     2761 gatgcgggcc acgctcgacc aggccatcca gttgtagccc agcgtgaagc ggagctgcgg
     2821 cgtgaaggcg tattcgagct tgacgccgac gtcgccgacg gccgcgaagt cttttcgttc
     2881 gtactggccg atgttcgagg gctgagcgaa cacgcccccc tcggtcaccg tgacgtcgtt
     2941 ggttggcgag atggtcgtcg tcgtgccgcg gacctgggcg atcgtccgcg tgccgccgag
     3001 ggcgaccttg cccgtcaggt tcaccgccca gcagcagttc caccagcgat agaccaggcc
     3061 gaactcgccg ccgtgaaagt cgttcttgga gctgaagctg tcgctgcggg caacgatcgt
     3121 atcggtgtcg aagccgctgg cgtcgtcgag cgacaggaaa atgctgtcgg cgctgaccgt
     3181 gtcttccagt cgcagatagc gccagccgcc gatcacgtcc agccgcgagc aggcctcgca
     3241 gccgagcatg tgccgcccga ggacttccag cccgaacagc tgcatgttcg aacgcacgcg
     3301 ggcttcgccg tccagcaggc ccggaaacga gatcagcacc gcgctgggca aatcggtggt
     3361 ggcatcgatg aacggcgtcg ccaggatcag gtcgtcgccg ccgatcgcat ggaagttgat
     3421 ccgctcgccg cccacccaca agagccgcgt ctcgacgccg tcgatgtgct ggggcgtgaa
     3481 ccagtagccg aagttaaacc gcgcgccgga gcggagcgtg ttctgcgtcg tgtcggcccc
     3541 gaataacacg gagccgtttg gaccaaggat tcccgcgtcg gccggatcgg tgccgctcgg
     3601 gctggtgctg atcagcgccg gcaggtgcgt cggcttgctg gcccaggcca ggtattccat
     3661 ttcggcccac gcccgcccca gcaccaacga ccgcacggtg cgggtgtcgc cgtagtagtt
     3721 gttgaagggg ttgtcgaact cgccgcaggg gctcttgccc tcgtagggcg cgaagaccat
     3781 gtccgcgccg ggcgtgtcgg cctgcggaaa ctgaagcggg gaggtcgccg ccatttcggg
     3841 ctcagcgagc gcgggcgcgg catgctgtgc cgccgcccca ctggccgcgc acagtagcca
     3901 tccgattgcc gcaatccgct tgagattcac ctagagcaag cctcccgcaa agtcggcgca
     3961 gtccgcaaat cctctatcgg cggaaaagtc ggcccagccc taacaatttg tctcaggcga
     4021 tccaactcat gctggctgtc gagcctggga gggtaaggat aattcccgcc ctccggcccg
     4081 attttcacac ctcccggagg ctataggcag ttcgcaactt ctttctcaca ttgagcttgc
     4141 gctcaatacc ctgtcgccag atggctgttt ggatttgaag cactagagag cccctttggg
     4201 aattatcctt acccggtttc agcctggggt tctgtcggtc ccgcctgccg ggcgggacct
     4261 cggcag
//
LOCUS       DASRJB010000069         7320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1715176, whole genome shotgun sequence.
ACCESSION   DASRJB010000069 DASRJB010000000
VERSION     DASRJB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715176"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFB80_01925"
     CDS             complement(<1..807)
                     /locus_tag="VFB80_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN32539.1"
                     /translation="MTAAFAIRARWVFPVDRPPIDGGVVSIREGRIEAVGQNTSGQPP
                     RDLGDVALLPGLINAHTHLQFSLLRHPLGRPGIPFADWIRQVIDFLRQSLPAGNDSAL
                     EAGLAESRAAGVAAVGEIAIRDWPSDLPQPPADMSVVVFRELLGLSEERVGPLADLAA
                     AHVEQSRAGQVAGLSPHAPYTVHPEVLRRACELSAARQVPVAMHLAETREELELLESH
                     SGPLVELLGSLGAWQPTALSRGLRPLDYLLTLATAHRALVIHGNYLAADEI"
     gene            911..1879
                     /locus_tag="VFB80_01930"
     CDS             911..1879
                     /locus_tag="VFB80_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZN32540.1"
                     /translation="MLKRILVTGGAGFLGSYLCERLVEAGHDVICLDNFFTSQKTNVA
                     HLLGRPNFDLVRHDITQPIYLEVDEIYNLACPAAPGHYQFNPIKTMKTSVLGAINVLG
                     MARRCKAKVLQASTSEVYGDPTEHPQSETYRGNVNPIGPRACYDEGKRAAEALFFDYH
                     RMNRINIRVVRIFNTYGPRMHPFDGRVVSNFIRQALGGDDITLYGDGQQSRSFCYRDD
                     LVEGIIRMMNGPDGFHGPCNLGNPVEFTVRQLAELVIELTGAKSKIVHEPLPADDPVR
                     RQPDIALARQHLGWEPATPLEGGLKKTIDWFRTIDFGDYRPPTPNY"
     gene            1919..2446
                     /locus_tag="VFB80_01935"
     CDS             1919..2446
                     /locus_tag="VFB80_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN32541.1"
                     /translation="MSNPKSQIPNPKFEHQRHKPPAPLSRPRDVVLCLAPMRSNVNLS
                     NIVRTAGCCGLTRLIACGSPKIDKTIARDGADQVAIEVHRTLPPVLKELKAAGFTLVG
                     LEQTTNSVSLHEFRFQRRTALVIGNERLGLTDEELRLLDAVVEIPVWGLPYAYNVANA
                     TAMALYEYCRQWPSG"
     gene            complement(2487..5003)
                     /locus_tag="VFB80_01940"
     CDS             complement(2487..5003)
                     /locus_tag="VFB80_01940"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN32542.1"
                     /translation="MSQHNGDHQSSEPAAEALTALVLNQLHGEERTAAEAQLAAGGEA
                     AQREVHEIQALSAALTAARSSQPLPQRSAELRQTIDNRLGQVELPPKRQLPWSVVAGV
                     SAVALASVLYILMLPAAQSAREGSRRVAALNRVRQAEVEAAAERQLATDSSAPVDINQ
                     WVTRDPASAPQPDATTLQSSVGSRRTKDGYLIAPPKTTNELHTQAPGNGQTVQLPPLA
                     FADKPASVDDYGKFPSVGGVAIDHDAIKFHATKRSDAGVVEAELARHIGDDATLKPAD
                     PKAAKSLTMMVTPRIIVQESEEAKLGIDLPDGTHRVQVWLDNGQSVYVTGTELAELVK
                     RTKERLLRQAEGRGTEQYERIIENAFLSPRQQPLSTFSIDVDTASYANVRRFLTGGSL
                     PPVNAVRIEELVNYFKYDYPQPKAGEPFSVNMEVAECPWQTGHLLLRVGLKGKEVAKQ
                     ERPPSNLVFLLDVSGSMSDENKLPLLKSAMKLLVGELGENDRVSIVTYAGEAGLRLPP
                     TRGHEQSKINAAIDSLSAGGSTNGSAGINLAYEQAAAYFAKGGTNRVILCTDGDLNVG
                     VTSDDALVELIKQKAAGGTFLTVLGFGEGNLKDAKLEKLADNGNGLYAYIDGLREARR
                     VLVEQLTGSTITIAKDVKIQIEFNPQEVAAYRLLGYENRVLAAEDFNNDEKDAGEIGA
                     GHTVTALYELVPAGGAEEPAPAAREQLKYQEPGARRQAPVTDHRSPTTDHRLTEAAKS
                     GELLTLKLRYKEPEGTESRLLEHPLKDRGGTFHAASRDMQFAASVASFGMILRGSQHR
                     GSGNLPAVLEIASTSLGDDTGGYRAEFVDLVRKAQSLGAR"
     gene            complement(5070..5654)
                     /locus_tag="VFB80_01945"
     CDS             complement(5070..5654)
                     /locus_tag="VFB80_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32543.1"
                     /translation="MEREQNRREWVLAALEQYEGRLLRYAQRLLGDLEAARDVVQFAF
                     LRLCDAQQADVEDRLAPWLFTVCRNKALDGRVAVVREANHSEAALCVDQLSREVDPAS
                     AAEDAELHGLLLLLIETLPPSQREALDLWAEGFSYTEIGGILDRQEGHIRVLVHRGLK
                     ALREHPQVRELLLEDDDSRSQAPLGTASAEAPLR"
     gene            complement(6139..7047)
                     /locus_tag="VFB80_01950"
     CDS             complement(6139..7047)
                     /locus_tag="VFB80_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32544.1"
                     /translation="MSFLVVRSHLARSRRSRRYGFTLVELLVVIAIIGVLIALLLPAV
                     QYARESGRRTQCQNNLRQMVIGMHNHADVHGFFPSAYEATSTNPGWGWGTAILPFVEQ
                     KNLYDAADVTRLRFGGGANPALPNTHTERKLSVYRCPSDPAPDKNPERLFHGMSNYRA
                     VMGPTESPLFFVDFDYGGVMYQNSRTRIARITDGTSNTLLIGECILDVKTGKRAALWA
                     GMTGLRAAPGTTTVSIWISDVMWPLDDVSANVNGPAPQAFSSRHPGGAFFAFCDGSVR
                     FFYNGGEKSLVKWMAGRDDGQVVQSP"
     gene            complement(7162..7320)
                     /locus_tag="VFB80_01955"
     CDS             complement(7162..7320)
                     /locus_tag="VFB80_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HZN32545.1"
                     /translation="MNGSAPQAFSSRHPGGAFFAYCDGSVRFFADTADMSLAKWAAGR
                     DDGNVTSP"
BASE COUNT         1258 a         2444 c         2309 g         1309 t
ORIGIN      
        1 gatttcgtcg gcagcgaggt aatttccgtg aatcaccagg gcgcggtggg ccgtggccag
       61 cgtcagcaga taatcgagcg gccgcaggcc gcgcgaaagt gcagtaggct gccaggcccc
      121 cagcgagccg agcaattcca cgagcggtcc ggaatgcgat tccagcagct ccaattcctc
      181 gcgcgtctcg gccaggtgca ttgctaccgg aacttgcctg gcagctgaca gctcgcaagc
      241 ccgccgcagt acctcggggt gcaccgtgta cggagcatgc ggactgagtc cggcgacctg
      301 ccctgccctg ctctgctcga cgtgggcagc cgcgaggtcg gcgagcggtc cgacgcgctc
      361 ttctgacagt cccagcagct cgcgaaacac gactacgctc atatccgcgg gcggctgcgg
      421 caaatcgctc ggccagtcgc ggatcgcaat ctctcccacg gcagccacgc ccgccgcccg
      481 cgactcggcc aggccggcct ctagtgccga atcgttcccg gcgggtaggg actgccgcag
      541 aaagtcgatg acctggcgaa tccaatcagc gaagggaatt cccggccggc ccagcgggtg
      601 ccgcagcagg ctgaactgca ggtgcgtgtg ggcgttgatc agccctggca ggagggccac
      661 gtcacccagg tcgcgcggcg gttgtccgct cgtattctgg ccgacggctt cgatccggcc
      721 ctcgcgaatc gacacgacgc cgccgtcgat cggcgggcga tcgaccggaa agacccagcg
      781 ggcgcgaatc gcgaaagcgg cagtcatggc accagcttag ggccgaggca aacaggcggc
      841 gatgacctat attctgattg ttaggtcact cacgactcgg gacaggccag aggcctatcc
      901 tacggaaatc atgctcaaac gcattctggt taccggcggc gcgggtttcc tcggctcgta
      961 tctctgcgag cggctggtcg aggccgggca cgacgtgatc tgcctcgaca atttcttcac
     1021 cagccagaag accaacgtcg cccacctgct gggccggccg aatttcgacc tcgtgcggca
     1081 cgacatcacg cagccgatct acctggaggt cgatgagatc tacaacctgg cctgcccggc
     1141 ggctcccggg cactatcagt tcaacccaat caagacgatg aagacctcgg tgctgggagc
     1201 catcaacgtg ctggggatgg cccgccgctg caaggccaag gtgctgcagg cttcgaccag
     1261 cgaagtctac ggcgacccga ccgagcatcc gcagagcgag acctaccgcg gcaacgtgaa
     1321 tccgatcggc ccgcgggcct gctacgacga aggcaagcgg gcggccgaag ccctgttctt
     1381 cgattatcac cgcatgaacc ggatcaacat ccgcgtcgtg cggattttca atacctacgg
     1441 gccgcggatg caccccttcg acggccgcgt cgtctcgaac ttcatccgcc aggccctcgg
     1501 cggcgacgac atcacgctct acggcgacgg ccagcagagc cgctcgttct gctaccgcga
     1561 cgacctggtc gaaggcatca tccgcatgat gaacggcccc gacggttttc acggcccgtg
     1621 caacctgggc aacccggtcg agttcaccgt ccgccagctc gccgagctgg tgatcgagct
     1681 caccggtgca aagtcgaaaa tcgtccacga gccgctgccc gccgacgatc ccgtccgccg
     1741 ccagcccgac atcgccctgg ccagacagca cctcggctgg gaacctgcca cgccgctcga
     1801 gggcggcctg aaaaagacca tcgactggtt ccgcaccatc gacttcggcg actaccggcc
     1861 gccaacgccg aactactgat tggggacttg ggattttcag atttgggatt tattgcacat
     1921 gtcaaatccc aaatcccaaa tcccaaatcc caaattcgaa caccagcgtc acaagccgcc
     1981 ggcgccgctc tcgcgaccgc gagacgtcgt cctctgcctc gcaccgatgc ggagcaatgt
     2041 caacctctcg aacatcgtcc gcaccgccgg ctgctgcggc ctcacccggc taatcgcctg
     2101 cggcagcccg aagatcgaca agaccatcgc ccgtgacggc gcggaccagg tagcaatcga
     2161 agtccaccgc acgctgccgc ccgtgctcaa ggagctgaaa gccgccggct ttacgctcgt
     2221 cgggctggag cagacgacca actccgtcag cctgcacgaa ttccgcttcc agcgccgcac
     2281 ggccctcgtc atcggcaacg agcgcctcgg cctgaccgac gaggaactac ggctcctgga
     2341 cgccgtcgtt gaaatcccgg tctggggcct gccgtatgcc tacaacgttg ccaacgcgac
     2401 ggcgatggcg ctgtatgagt attgccggca gtggccgtcg ggttagccgg agcgagagaa
     2461 ggagagtacg cctctgtcgc tccgtcctat ctcgccccca gcgactgcgc cttgcggacg
     2521 agatcgacga actccgcccg atagccgccg gtgtcgtcgc cgagcgaggt gctggcgatc
     2581 tccaggacgg cgggaaggtt gccgctgccg cgatgctggc tgccgcggag gatcatgccg
     2641 aagctggcga ccgaggcggc gaactgcatg tcgcgcgagg ccgcgtggaa cgtgcccccg
     2701 cggtccttca gcgggtgctc cagcagccgg ctctcggtcc cctcgggttc tttgtagcgt
     2761 agcttgagcg tgagcagctc gccggacttg gcggcttcgg tgagtcggtg gtcggtggtc
     2821 ggtgatcggt ggtcggtgac tggcgcctgg cgcctggctc ctggctcctg gtacttgagt
     2881 tgctctcgcg ccgcgggggc cggctcctca gcaccgccgg ctggcaccag ctcgtacagc
     2941 gcggtgaccg tgtggcccgc gccgatctcg ccggcgtctt tctcgtcgtt gttgaaatct
     3001 tcggcggcca gcacgcggtt ctcgtagccg agcaggcggt aggcggcgac ttcctgcggg
     3061 ttgaactcga tctgaatctt cacgtccttg gcgatcgtga tcgtgctgcc ggtgagctgc
     3121 tcgacgagca cgcggcgggc ctcgcggagg ccgtcgatgt aggcgtaaag gccgttgccg
     3181 ttgtctgcca gcttctccag cttggcgtcc ttcaggttcc cttcgccgaa gccgagcacg
     3241 gtcagaaacg tgccgccggc ggccttctgc ttgatcagct cgaccagggc gtcgtcgctg
     3301 gtgacgccga cgttcaggtc gccgtcggtg cagaggatca cgcggttcgt cccgcccttg
     3361 gcgaaatagg ccgcggcctg ctcgtaggcc aggttgatcc ctgcgctgcc gttggtcgag
     3421 ccgccggccg agagcgaatc gatcgccgcg ttgatcttcg actgttcgtg gccgcgcgtc
     3481 ggcggcagcc gcaggcccgc ctcgccggcg taggtgacga tcgacacgcg gtcgttctcg
     3541 cccagctcgc cgacgaggag cttcatggcc gacttcagca gcggcagctt gttctcgtcg
     3601 ctcatcgagc ccgacacgtc gagcaggaac accaggttgc tcggcggccg ctcttgttta
     3661 gccacttcct tgcccttgag gccgacccgc agcagcaggt gccccgtctg ccacgggcac
     3721 tcggccactt ccatgttcac cgagaacggc tcgcccgcct tcggctgcgg atagtcgtat
     3781 ttgaaatagt tcaccagctc ttcgatccgc accgcgttca cggggggcag gctgcccccg
     3841 gtcaaaaacc gccgcacatt ggcatacgag gccgtatcca cgtcgatcga gaacgtcgag
     3901 agcggctgct gccgcggcga gaggaacgcg ttctcgatga tccgctcata ctgctccgtt
     3961 ccgcggcctt cggcctgtcg gaggaggcgc tccttggtcc gcttgacgag ctcggcgagc
     4021 tcggtgcccg tcacgtacac gctctgccca ttgtccaacc acacttgcac cctgtgcgtt
     4081 ccatcgggca ggtcaattcc cagcttcgcc tcctcgctct cttgaacgat gattctaggc
     4141 gttaccatca tggtcagcga tttggccgcc ttcgggtccg ccggcttgag ggtcgcatca
     4201 tccccgatgt gcctggccaa ttccgcctcg accacgcctg cgtcagaacg tttcgtcgcg
     4261 tggaatttga tggcatcgtg gtctattgcc acgccgccga cggatgggaa cttcccatag
     4321 tcgtcaacgg acgcaggctt gtccgcgaag gcaagcggcg gcagctgcac cgtctgccca
     4381 ttgccaggcg cctgagtatg caactcgttc gtcgtcttcg gcggggcgat caaataaccg
     4441 tctttggtgc gccggctgcc gacggaagat tgcagagttg ttgcatcagg ctgaggcgca
     4501 gaggcgggat cgcgggtaac ccactggttg atgtccacgg gtgcagagct gtccgtggcc
     4561 aattgccttt cagccgcggc ttccacttcg gcttggcgca cccgattgag cgccgccaca
     4621 cgccgcgatc cctcgcgggc tgattgcgcc gctggcaaca tcagaatgta gaggaccgac
     4681 gccagcgcga cagccgacac gccggccacg acgctccaag gcagttggcg cttcggcggc
     4741 agctcgacct ggcccagccg attgtcgatt gtctggcgga gctcggccga tcgctgcggc
     4801 agcggctggc tgctgcgggc ggcagtgaga gcggcggaga gggcctggat ttcgtggact
     4861 tcgcgctggg cggcttcgcc gccggccgcg agctgcgcct cggctgcggt tcgctcttcg
     4921 ccatgcagct ggttcaggac cagcgccgtg agcgcctcgg ccgccggctc gctcgactgg
     4981 tgatcgccgt tgtgctggga catgagtatc tctcctcgtt cccaagctct gcttgggaac
     5041 gcacatctgg aagctctgct tccaggtctt tagcgaagcg gagcttcggc acttgcggtc
     5101 ccaagcggag cttgggaccg agaatcgtcg tcctccaaaa gcaattcccg cacctgcgga
     5161 tgctcgcgga gggccttcag gccgcggtgc acgagcacgc ggatgtggcc ttcctgccgg
     5221 tcgaggatgc cgccgatctc ggtgtagctg aacccctcgg cccacaggtc tagggcttcc
     5281 cgctggctcg gcggcagcgt ttcgatcagc agcagcaaca agccgtgcag ctcggcgtct
     5341 tccgcggccg atgccggatc gacctcgcgg ctcaattggt cgacacacag tgccgcttcg
     5401 ctgtggttcg cttcgcgaac tacagctacc cgtccatcca gcgccttatt gcggcagacg
     5461 gtgaacagcc acggggccag ccggtcttcc acgtcggcct gctgcgcgtc gcacagccgc
     5521 aggaaggcga actgcacgac gtcgcgggcc gcctcgaggt cgccgagcag gcgctgggca
     5581 tagcgcagca gccgaccctc gtattgctcg agggcggcca gcacccattc ccggcggttt
     5641 tgctcacgtt ccatcgcagc tcattcccgg cccaccaata caacgacgcg gcgacgtttt
     5701 cgttacgaga cagaggcaac atggccccct gaattggtcg tccgaacttg ccgttcgctc
     5761 gcgagttttg ccacaaggct ttgcagagca aagacttgtg acgcgggttt gaaaaaccct
     5821 taagtagtac ttattaggct ttgagacagg ctgtctttgt cgcgcgccgg ggagcggcga
     5881 agccggcgca aaccggtagc gcgaaatggc gcatcgcgcc ccgttatcgc gaccgcggcg
     5941 cgatgcgccg tcgcctgctt cgaatttcca aagaacctgc cgcccgccaa cggaccggcc
     6001 agccagttag aattgtacat ttccgtccag tatatgtcaa gttaaccaca acagattgtg
     6061 gtcgccttcc gccagaccac aatgggttgt ggtcggcgcg cgcagaggcc cccgaaagag
     6121 ggcccgccgg cttgctccct acggcgactg gaccacctgg ccgtcgtcgc ggccggccat
     6181 ccacttgacg agcgactttt cgccgccgtt gtagaagaac cgcaccgagc cgtcgcagaa
     6241 cgcgaaaaag gccccgccgg ggtgccggct gctgaaggcc tgcggagcgg gaccattgac
     6301 gttggcgctg acgtcgtcca ggggccacat cacgtcgctg atccagatcg aaacggttgt
     6361 ggtgccaggg gctgcgcgca atcccgtcat gcccgcccac agcgcggccc gcttccccgt
     6421 cttcacgtcg agaatgcact cgccgatcag cagtgtgttt gaagtgccgt ccgtgatccg
     6481 ggcgattcgc gtgcggctgt tctgatacat cacgccgcca tagtcgaaat cgacgaaaaa
     6541 cagaggagac tcggtcggac ccatcacggc ccggtagttc gacatcccgt ggaacaagcg
     6601 ctcgggattc ttgtcgggcg cgggatccga cgggcagcgg tacacggaca gcttgcgctc
     6661 ggtgtgcgta ttgggcaggg cggggttggc gccgccgccg aacctgagcc gcgtgacgtc
     6721 cgcggcgtcg tacagattct tctgttcgac aaatggcaga atggccgtgc cccagcccca
     6781 gccgggattg gtgctggtcg cttcgtacgc cgaggggaaa aagccatgga cgtcggcatg
     6841 gttatgcatg ccgatgacca tctgccgcag gttattctgg cactgggttc gccgccccga
     6901 ctcgcgggcg tactgcaccg cgggcagcag cagagcgatc agcacaccga taatggcgat
     6961 gacgacgagc agctcgacca gcgtaaatcc gtagcggcgg ctccgcctgg agcgcgcaag
     7021 atgcgatcgc accacaagga atgacatgtt gtgagccccc aaggaagatc gtgttgcctc
     7081 acctcccctc ccctttgccc gctccgaaga taatggaacc agctggcaca atcaagcgaa
     7141 aacaagccgg aacgccactt ttcagggcga ggtcacattg ccgtcgtccc gcccggccgc
     7201 ccacttcgcc agcgacatgt ccgcggtgtc cgcgaagaac cgcaccgagc cgtcgcaata
     7261 ggcgaagaag gcgccgcccg gatgccggct ggagaaggcc tggggagcgg agccgttgat
//
LOCUS       DASRJB010000070         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2844269, whole genome shotgun sequence.
ACCESSION   DASRJB010000070 DASRJB010000000
VERSION     DASRJB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844269"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFB80_01960"
     CDS             <1..882
                     /locus_tag="VFB80_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32546.1"
                     /translation="SIQLRRPGEYAGWLGRGYDPLTTAIGKRDKNDNPYFRDCTDEEL
                     NYQIDGLALPGDITLDRAARRQTLLAQFDEQLRAAENAPALAALDKFQSRAFQLATSH
                     ETRQALDIRQEPAALRDRYGRNLFGQSVLLARRLVEAGVRYVTVHFDAVDGYSWDSHV
                     HSDDVKKFLLPALDNALASLLEDLAQRGLLDETLVVCLGEMGRTPQGTDRWGRGHWST
                     LFPAVLAGGGIRGGIVYGASDKDAAYPVDRPTKPEDLAATIYEALGIDHHLQLPNAQG
                     RPVTIVEGGRPLTELFG"
     gene            complement(968..2152)
                     /locus_tag="VFB80_01965"
     CDS             complement(968..2152)
                     /locus_tag="VFB80_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN32547.1"
                     /translation="MNLTSRVLAGCLMATLASTARAADLPGLVDQQPASGRFVKTDRG
                     FMVPYQTMIPGTDVTFQMQPIPGGTFRLGSPAGEKGHKPVEEPQIEVKIEPFWMGAHE
                     VTWAEYKNYMSMHDLFKKLQAAKLHPVTDANKHLIVTAPSNLYDSTFTFKLGSEPNLP
                     AVTMSQYAAKQYSKWLSGLTGQVFRLPTEAEWEYACRAGTSTAYSFGDDPAQLGDYGW
                     FFDNSGERMHQVGQKKPNPWGLFDMHGNVGEWVLDAYTPDGYARLKDKGVVNWDGAIG
                     WPKKLYPRVVRGGGFEDDAPGCRSAARRKSDDDDWREEDPNFPQSPWWFTSQPALSVG
                     FRLIRPLVEPQLAERGKYWDADLAQLQEDIDHRIDNEGRGARGVASPELPDAIKRLSG
                     PK"
     gene            2337..2723
                     /locus_tag="VFB80_01970"
     CDS             2337..2723
                     /locus_tag="VFB80_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZN32548.1"
                     /translation="MTAIDPNYPFEAGDYAAAAAVRPDDDLEMAERLQRLPAYSRSLL
                     RIAVPVVVTLAMTRRPVSSVLNLAPGTILQFSKPCDEPLTLSVGGCDVAVGDTVKVGE
                     KFGLKITSMVMPVEKFETVQQPRERR"
     gene            complement(2732..>3557)
                     /locus_tag="VFB80_01975"
     CDS             complement(2732..>3557)
                     /locus_tag="VFB80_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32549.1"
                     /translation="DVYNQLPPLRVNNNRATWFVLIMPYMEQENIERLWRYDQDYSNA
                     ANVAGRALQVKSYYCPSRRGPQTSPNISRQEDVRPGDNGPPPDFTGPFTDARFFGTNN
                     PPGALGDYAGNVGNVNNWPFAPTDVSWASIRANGAIVQAATFDTASGRWTSGTAFRTV
                     TDGLSNTYLAGEKHVPAGMFGRAKVGDGSIYNGVWSTYSGRVAGPEDPPAKGPKDISA
                     STTADAFFARKFGSYHPGVTQFAFCDGSVRATKITINLDNYRRLACRHDGEAVTED"
BASE COUNT          574 a         1154 c         1224 g          605 t
ORIGIN      
        1 tcgatccagc ttcgccgccc cggcgaatac gccggctggc tgggccgcgg ctacgacccg
       61 ctgacgacgg cgatcggcaa gcgcgacaaa aacgacaacc cgtacttccg cgactgcacc
      121 gacgaggagc tgaactatca gatcgacggc ctggcgctgc cgggcgatat cactctcgac
      181 cgcgccgcac ggcggcagac gctgctggcc cagttcgacg agcagctccg cgcggccgaa
      241 aacgcgccgg ccctggcggc cctcgacaag ttccagtcgc gggcctttca gctggccact
      301 tcgcacgaga cgcggcaagc gctcgacatc cgccaggagc cggcggcgct gcgcgacagg
      361 tacggccgca acctgttcgg ccagtcggtg ctgctggccc gccggctggt tgaggccggc
      421 gtacgctacg tcaccgtgca cttcgacgcg gtggacggct acagctggga ttcgcacgtc
      481 cacagcgacg acgtgaagaa gttcctgctg ccggcgctcg acaatgccct ggcgtcgcta
      541 ctggaggacc tcgcgcagcg cggccttttg gacgagacgc tcgtcgtctg cctgggcgag
      601 atgggccgca cgccgcaggg caccgaccgc tggggccgcg gccactggag cacgctcttt
      661 cccgcggtcc tcgccggcgg cggcatccgc ggcggcatcg tctacggcgc cagcgacaag
      721 gacgccgcct atccggtcga tcggccgacg aagcccgaag acctggccgc gacgatttac
      781 gaggccctcg gcatcgacca tcacctgcag ctgccgaacg cgcagggccg ccccgtcacg
      841 atcgtcgaag gcggccggcc gctgaccgag ctgtttgggt aactggactc gtttaacccg
      901 tagccggagg cgacggcgcc ctggggaaag gcctgcgcct ccggcttcgg gttaaacgac
      961 attggcgcta cttcggcccg ctcagccgct tgatggcgtc gggcagttcc gggctggcga
     1021 cgccgcgagc gccgcggcct tcgttgtcga tgcggtgatc gatgtcctcc tgcagctgcg
     1081 ccaaatcggc gtcccaatac ttgccgcgct cagccaactg cggctcgacc agcgggcgaa
     1141 tcagccggaa gccgacggag agcgcgggct ggctggtgaa ccaccagggg ctttgcggaa
     1201 agttcgggtc ttcctcgcgc cagtcgtcgt cgtcgctctt gcggcgggcg gccgagcggc
     1261 agccgggggc atcgtcctcg aagccgccgc cgcggacgac gcggggatag agcttcttcg
     1321 gccagccgat cgcgccatcc cagttgacga cgcccttgtc cttgaggcgg gcgtagccgt
     1381 cgggcgtgta ggcgtcgagg acccactcgc cgacgttgcc gtgcatgtcg aagaggcccc
     1441 acggattggg cttcttctgc ccgacctggt gcatccgctc gccggagtta tcgaagaacc
     1501 agccatagtc gccgagctga gcggggtcgt cgccgaagga atacgcggtg gacgttccgg
     1561 cgcggcaggc atactcccat tcggcctcgg tgggcaggcg aaagacctgc ccggtgagcc
     1621 cgctaagcca cttgctgtac tgcttggccg cgtactggct catcgtcacg gcaggcaggt
     1681 tcggctccga gccgagcttg aacgtgaacg tcgagtcgta gaggtttgag ggggccgtga
     1741 cgatcaggtg cttgttcgcg tcggtgacgg ggtgcagctt ggccgcctgc agcttcttga
     1801 acaggtcgtg catcgacatg tagtttttgt attccgccca ggtcacctcg tgcgcgccca
     1861 tccagaacgg ctcgatcttg acttcgatct gcggctcttc gaccggtttg tggcccttct
     1921 cgccggcggg gctgccgagc cggaacgtgc cgcccgggat cggctgcatc tggaacgtca
     1981 cgtccgtccc gggaatcatg gtctggtagg gcaccatgaa gccgcggtcg gttttgacga
     2041 accggccgct agcaggctgc tggtccacga ggccgggcag atcggcggcg cgggcagtgc
     2101 tggccagagt cgccatcagg cagccggcca ggacgcggga agtaagattc atgagagccg
     2161 ctcccggaga tttgccgcca agaattgcgt tcattttagt ctgcggcccc aaaccagcga
     2221 aggcgggcaa gctggcgaaa accaacgcgg cgggcgaagt gcggcgggcg aaactacgca
     2281 aagtttgccg ctgccagcgg ccgataatga cgccgagcgc ccgcgaacag ccaaccatga
     2341 ccgccatcga tcccaactat ccgttcgaag ccggcgacta tgcagcagcg gcggcggtgc
     2401 gcccggacga cgacctggaa atggccgagc ggctgcagcg gctgccggcc tattcgcgga
     2461 gtttgctgcg gattgctgtg ccggtggtcg tgacgctcgc catgacgcgc cggccggtga
     2521 gcagcgtcct gaatctggcc ccgggcacga tcctgcagtt cagcaagccc tgcgacgagc
     2581 cgctgacgct ctcggtcggc ggctgcgacg tggccgtggg cgacacggta aaggtcggcg
     2641 agaaattcgg cctgaagatc acctcgatgg tcatgcccgt cgagaagttc gaaaccgtgc
     2701 agcagccgcg agaacggcgg tagcccacgg ctcaatcttc ggtcacggct tctccgtcgt
     2761 ggcgacaggc caaccggcgg tagttgtcga ggttgatcgt gattttcgtg gcccgcacgc
     2821 tgccgtcgca aaacgcgaac tgcgtgacgc cagggtggta gctgccgaac ttgcgggcaa
     2881 agaacgcgtc ggccgtcgtg ctggctgaaa tgtccttcgg gcccttggcc ggcggatctt
     2941 ccggccccgc gacgcggccg gagtaggtgc tccacacgcc gttgtagatc gagccgtcgc
     3001 ccactttggc gcgcccgaac atgccggccg gcacgtgctt ctcgccggcg aggtaggtgt
     3061 tgctgagccc gtcggtgacg gttcgaaagg cggtgccgct ggtccagcgc cccgacgcag
     3121 tgtcaaacgt ggccgcctgc acgatagccc cgttggcgcg gatcgaagcc caggagacgt
     3181 cggtcggcgc aaacggccag ttgtttacgt tgccgacgtt gccagcgtag tcgccgagtg
     3241 ctcccggcgg attgttcgtg ccaaaaaaac gggcgtcggt gaaaggccct gtgaaatccg
     3301 gcggcggacc gttatcgcct ggccgcacat cctcctgccg gctgatgttg ggactggtct
     3361 gcgggccgcg gcggctgggg cagtagtacg atttgacttg aagggcgcgg cccgctacgt
     3421 tcgccgcgtt ggaatagtcc tggtcgtagc gccacaggcg ctcgatattc tcctgctcca
     3481 tgtagggcat gatcagcacg aaccaggtgg cgcggttgtt gttcacgcgg agcgggggca
     3541 gctgattgta gacgtcg
//
LOCUS       DASRJB010000071         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3125941, whole genome shotgun sequence.
ACCESSION   DASRJB010000071 DASRJB010000000
VERSION     DASRJB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125941"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFB80_01980"
     CDS             <1..912
                     /locus_tag="VFB80_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32550.1"
                     /translation="SAAVRRSRAGLQDQHRPIGSFLFLGPTGVGKTELCKALAEVMFD
                     SDQSVVRIDMSEFGEKHNVSRLIGAPPGYIGYEEGGRLTEAVRRRPYSVVLLDEIEKA
                     HRDVFNVLLQVLDDGRLTDGQGRTVDFTNTIIVMTSNIGSQMIQQIASEGGKAEEIEE
                     AVREILKEKFLPEFLNRIDETIVFHPLTREQIGKIVELQLKRLEATLAERDLRVEITD
                     AAKKAIANEGYDPIYGARPLKRVIQQRLQNPLSTELLKGQIPEHGGVRIDHDGDEFVF
                     KPLPPAAPSSGKSDGKGRTKVETVGVR"
     gene            982..1248
                     /locus_tag="VFB80_01985"
     CDS             982..1248
                     /locus_tag="VFB80_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32551.1"
                     /translation="MRPEELTQMLRRQPFTPFRIHVTTGQTYEIRHPELVWVRRQCAD
                     VALDPDPKTGVIEHTERVSLLHIVRIEDVEAPVGPAKGNGEASA"
     gene            1331..2332
                     /locus_tag="VFB80_01990"
     CDS             1331..2332
                     /locus_tag="VFB80_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32552.1"
                     /translation="MRRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMSCANNL
                     KQIGLGFHNYESTFRVLPPQTMATSTGFHGATAFVFVLPQIEQGPLIDRLSGVGFGSQ
                     ISYWMGSGSAKTALVRPILDGYQVNAYRCPSTQLPRFRDVPFNGVNFRYMVPSYVMIA
                     GSNLSSTTDTNGPNGGHCSGGGIFPGSVARRFAEISDGTSNTMMLAEQSNWVARQLNN
                     FRTAFEGSGPWMGVKNARLPNGPGTWSATGTHDSNGIDTDMRSYAMTTVRDGPNPKGT
                     AGYMNNVACNTPLASAHPNGVQAALADGSVRFIPDSIDLTTLKRLADRDDGNALGDF"
     gene            2337..2732
                     /locus_tag="VFB80_01995"
     CDS             2337..2732
                     /locus_tag="VFB80_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN32553.1"
                     /translation="MRAARVCFLAALALAGCSSDGPARFAVTGKVTFQGQPVEEGQIT
                     FEDSAAGQVNSGKLGSGGSYSVELPAGDYRVSVAPPLVETPGTRDSPGDMVPKKVKNI
                     PKKHWVPESSRLTAKVNSSARSFDFELGP"
     gene            2823..>3340
                     /locus_tag="VFB80_02000"
     CDS             2823..>3340
                     /locus_tag="VFB80_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HZN32554.1"
                     /translation="MTNQPPNAERRRLADANRGIVRWRYWGPYLSERQWGTVREDYSP
                     EGTAWDYFPHDHARSRTYRWGEDGLAGISDERGRLCLALALWNGRDPILKERLFGLTN
                     GEGNHGEDVKELYYYLDATPTHSYLKMLYKYPQAEFPYERLIEENRRRGRDQPEFELL
                     DTGLFDGDRYFDV"
BASE COUNT          640 a         1101 c         1076 g          523 t
ORIGIN      
        1 agcgccgcgg tccgccgcag ccgggccggc ctgcaggacc agcatcggcc gatcggctcg
       61 ttcctgttct tgggtccgac cggcgtgggc aagaccgagc tgtgcaaggc cctggccgag
      121 gtgatgttcg actccgacca gtccgtggtc cgcatcgaca tgagcgagtt cggcgagaag
      181 cacaacgtca gccgcctgat cggcgctccg ccgggctata tcggctacga agaaggcggg
      241 cgcttgaccg aggcggttcg ccgccggccg tacagcgtgg tcctgctcga cgagatcgaa
      301 aaggcccacc gggacgtatt caacgtgctg ttgcaggtgc tcgacgacgg ccggctgacc
      361 gacggtcagg gccgcaccgt cgatttcacg aacacgatca tcgtgatgac cagcaacatc
      421 ggcagccaga tgatccagca gatcgccagc gagggcggca aggccgaaga gatcgaggaa
      481 gcggtgcggg aaatcctcaa ggagaagttc ctgccggaat tcctgaaccg gatcgacgag
      541 acgatcgtat tccatccgct cacccgcgag cagatcggca agatcgtcga gctgcagctc
      601 aagcggctgg aggccacact cgccgagcgc gacctccgcg tggagatcac cgacgccgcg
      661 aaaaaggcca tcgccaacga gggctacgat ccgatctacg gcgctcgccc gctgaagcgc
      721 gtgatccagc agcggctgca aaacccgctc tccaccgagc tgctcaaggg ccagatcccc
      781 gagcacggcg gcgtgcgcat cgaccacgac ggcgacgaat tcgtcttcaa gcccctcccg
      841 cccgccgctc ctagcagcgg caagtcggac ggcaaggggc ggacgaaagt cgagacggtc
      901 ggcgtgcggt agcgtagtgc tcgcggaagc gcgtggattg ccattcaagc cccgtctaga
      961 atgaggcgag gaggccaaca cgtgcgcccc gaagaactga cccagatgct ccgccggcag
     1021 ccgttcacgc cgttccgcat tcatgtcacg acgggacaaa cttacgagat tcgccatcca
     1081 gaactggttt gggtgcggcg gcaatgcgcc gatgtggctc tcgatcccga tccaaagacg
     1141 ggcgttatcg aacacaccga gcgagtgtcc ctcctgcaca tcgtgcggat cgaggacgtg
     1201 gaagcacccg tcggacccgc caaaggcaac ggcgaagcat cggcctgaat ggctttgcat
     1261 ggccgctccg cgtccgccgc tttatgataa tggcgatagc gctgcggatt ctggagtggg
     1321 agacatcgcc atgcgccgag catttactct ggtcgagttg ctggtggtga tcgcgatcat
     1381 cggcgtgctg gtcgcgctgc tgttgccggc ggtgcaggcg gcccgcgagg cggcgcggcg
     1441 gatgagctgc gcgaacaacc tcaagcaaat cgggctgggc tttcacaact acgagagcac
     1501 gttccgcgtc ttgcccccgc agacgatggc cacgagcacg ggctttcacg gcgcgacggc
     1561 cttcgtcttc gtcctgccgc agatcgagca aggcccgctg atcgaccgcc tgtccggggt
     1621 cgggttcggc tcgcaaatca gctattggat gggctcgggc agcgccaaga cggcgctggt
     1681 gcggccgatc ctcgacggct atcaggtgaa cgcttaccgc tgcccctcga cgcagttgcc
     1741 gcggttccgc gatgtgccct tcaatggcgt gaacttccgc tacatggtgc cctcgtacgt
     1801 gatgattgcc gggagcaacc tcagcagcac gaccgacacc aacggcccca acggcggcca
     1861 ttgctcgggc ggcggcattt tccccggcag cgtggcccgg cggttcgccg aaatcagcga
     1921 cggcacctcc aacacaatga tgctcgccga gcagtcgaac tgggtcgcca ggcagctcaa
     1981 caactttcgc acggccttcg aaggcagcgg tccctggatg ggcgtcaaga acgcccgcct
     2041 gcccaatgga cctggcacct ggtccgcgac gggcacgcac gactccaacg gcatcgacac
     2101 cgacatgcgt tcgtacgcga tgacgacagt ccgcgacgga cccaatccca aggggacggc
     2161 cggctacatg aacaacgtgg cgtgcaacac gccgctggcc tcggcccatc ccaacggcgt
     2221 gcaggccgcc ctggccgacg gcagcgtgcg cttcataccc gattccatcg acctgaccac
     2281 gctcaagcgc ctcgccgacc gcgacgacgg caacgccctg ggagactttt agccgcatgc
     2341 gggccgcacg cgtttgcttt ctggccgcgc tggcactcgc cggctgctcg tccgacggtc
     2401 ccgcgcgctt cgccgtgacg ggcaaggtga cattccaggg acagccggtg gaagaaggcc
     2461 aaatcacgtt cgaagattcc gcggccggcc aggtgaactc cggtaaactt gggtcgggcg
     2521 gcagctactc cgtcgaattg cccgcggggg attatcgcgt cagcgtcgcg ccgccgctgg
     2581 tcgaaacgcc gggtacccgc gattcgcccg gcgacatggt gccgaaaaaa gtaaagaaca
     2641 tccccaaaaa acactgggtt cccgagtcgt cgcgcctcac cgccaaggtg aattccagcg
     2701 cacgatcgtt cgacttcgaa cttgggccct agctccgcca gccatggata cgatcggcgg
     2761 caagctatga ttgtggagcg gaaggaccgc ggccgaatcc cgatgccgcg cgccagaccc
     2821 cgatgacaaa tcaacctccg aatgccgagc gacgccgcct ggcggatgcc aaccgcggca
     2881 tagtgcgctg gcggtattgg gggccgtatt tgtcggagcg gcagtggggg acggttcgcg
     2941 aggattacag cccagagggc acggcctggg actatttccc gcacgaccac gcccgcagcc
     3001 ggacgtatcg ctggggcgag gacggactgg cgggcatctc ggacgaacgg ggccggctct
     3061 gcctggcgct ggccctctgg aacgggcgcg acccgatcct caaggagcgg ctgttcggct
     3121 tgaccaacgg cgaaggcaac cacggcgagg atgtcaagga gctctactac tatctcgacg
     3181 ccacgccgac gcactcgtac ctgaagatgc tctacaagta tccgcaggcc gagtttccct
     3241 acgagcggct gatcgaggag aatcgccgcc gcggccgcga tcagccggag ttcgagctgc
     3301 tcgacacggg cctgttcgac ggcgaccgct acttcgacgt
//
LOCUS       DASRJB010000072         6612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3689786, whole genome shotgun sequence.
ACCESSION   DASRJB010000072 DASRJB010000000
VERSION     DASRJB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3689786"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VFB80_02005"
     CDS             <1..1527
                     /locus_tag="VFB80_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HZN32555.1"
                     /translation="QMMLGRGEYGGARILSPLTVAAMTRGYRLAAGSRSTMEGVPANP
                     PAYFRGPGWDKRTGYSINRGELLSDAAFGHGGFTGTVLWIDPELDLFFIFLSNRVHPD
                     GKGLVNPLAGRIATVAAAAIRDVEKRTAAGTVLTGIDVLKRDGFKQLAGRKLGLITNH
                     TGKSREGETTIKLLHEAPNVELRALFSPEHGFEGKLDVSKIGDTRDAATGLKVFSLYG
                     ETRKPTPEMLAGIDTLVFDIQDIGARFYTYTSTMGEALKACAEHGKRFVVLDRPNPIG
                     GTKVAGPMLDAGKESFVGFHKLPVRHGMTIGELARLFQEELKLQVDLQVIQCEGWKRA
                     DAFDATGLTWVNPSPNMRSLTQAFLYPGIGLLETTNLSVGRGTDTPFEVIGAPWLEGQ
                     KLAAELSARGIPGVGFVPIEFTPTSSKFANQKCGGVNILITDRSRFEPLRTGFEIAAQ
                     LRKLYPDKWEAKGYDRLLGNEKTLQALLEGKPAEELEAVSRVGVAEFVRSRSRVLIYD
                     "
     gene            1679..3721
                     /locus_tag="VFB80_02010"
     CDS             1679..3721
                     /locus_tag="VFB80_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN32556.1"
                     /translation="METRNLLATFFVANTGNDGNDGGSAAPWLTLQKAANSVQAGDTV
                     IVRAGTYAGFDLRTDGTAANRIVFQGEAGATITSRNPITPDGINLEGADYVTIEGFKV
                     VGMPRTGIRSVLNHHVIIRNNECDLNQSWGILTGFSDDILIENNITTRSVVEHGIYFS
                     NSGDRPIIRNNVSWGNRANGIHMNGDVSLGGDGIISNALVEGNVIYGNGVGGGSGINC
                     DGVQNSTIRNNLIYDTHASGISLYQIDGGGGSSGNLVVNNTVIVAADGRWALNIQSGS
                     TGNTVRNNILYTAHSFRGSIDISADSLAGFSSDYNVLMNRLTTNGGDSVQTLAQWQAS
                     SGQDAHSLVATPSQLFVNAAGFDFHLSAASAAVDAGTTQFAPLIDFEGHARPSGSGID
                     IGADELSGGPPPPPPPPTNHAPTNVALSGSTIKETAAAGSVVGTLSVADPDAGDTHTL
                     LLVNDAGGRFALSGNQLALAGPINYEAAASHSIVVRATDAGGLSFDKTLVVGVTNVNE
                     LVRIDVQKGAVQRSYVRYVDLIFESSAGLSQLISEGRINLTRFTLAGASPVNVALAGK
                     LKVSGNRIIADFGTNGIGGNRNTATGNGYYRFGVDADRNGTKETQKHFYRLFGDTNGD
                     RTVNATDQANVNADRGKTGSNLRSDLNGDGFVNATDLTYVKAQLGRTLWSALPLDD"
     gene            3964..4641
                     /locus_tag="VFB80_02015"
     CDS             3964..4641
                     /locus_tag="VFB80_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZN32557.1"
                     /translation="MRFPKLVLLLCAAAWAITPQAQVQGGIFTLTNATSGIFDASSGT
                     RSVTVTGLEVGFDGGVLTNITVAIDYLKADGEAADPPPPPPLPGSPFFNEIHFRLDRA
                     ANPSTELIAAGHYGVGGAGDFFSGIQTFATGGAIIPAGGSPVAGTFAPTGPGSLAAYN
                     GASALGVWTLFVEDTVGLDALRFRSVTFTFTTESAAVPEPSSCAILGAGVVGLLGFCR
                     RRRQLAA"
     gene            4733..5467
                     /locus_tag="VFB80_02020"
     CDS             4733..5467
                     /locus_tag="VFB80_02020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN32558.1"
                     /translation="MSKLNGKVALITGAGRGIGRGIALAFAGEGAVLVLTARSREQLE
                     SVAREAAQLSKAPVIVQPADVSDEQQVESLFARVKAEFGRLDILVNNAGAFDGGPLDQ
                     LSTEAWDRVINTNLRGPFLCTRAALRMMKPVGSGRIINIGSIAAQRVRPNSAPYAASK
                     HGLWGLTQATALEGREYGVTCGAVHPGNVMVERRAGSGRGEDDEPMMTPAEIAEVALL
                     MATLPPHVELLEAIVLPHRQHYVGRG"
     gene            5511..6578
                     /locus_tag="VFB80_02025"
     CDS             5511..6578
                     /locus_tag="VFB80_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32559.1"
                     /translation="MASPIRLAFLGIDHPHGAHWRQQLANFTGEVEIVAFVPSFGGGT
                     TSLEERYAELPRFESVSALLKGAQFDAAFVGLANRDGPPAIVELAAAGKHILAEKPVA
                     ASAADARPMVAAVEKSGVAYQAGYMWRYDDIANRLKRMLQQDAFGKLISVEMTFATSD
                     VARRGPEHYLFDPAASGAGFFSWLACHWLDTLFFVTGRAVVGVTARTGVFGAVPTKVE
                     DGGAAILELDGGGLATFVGGYWHPRWAGESRWAIRGSQRWVHWDANRKGTNGALEIHG
                     PQPQWHAMEDVFSTPEDKSPGYGGRRGFLLVQDWLDCIRTCRRDNRATAHGTLATLEL
                     IDAIFQSSREGKRIECRIEPA"
BASE COUNT         1178 a         2176 c         2111 g         1147 t
ORIGIN      
        1 cagatgatgc tcggccgcgg cgaatacggc ggcgcgcgaa ttctgtcgcc gcttaccgtc
       61 gctgccatga cccgcggtta ccgcctggcg gccggctcgc ggagcacgat ggagggcgtt
      121 cccgccaatc cgccggccta tttccgcggc ccggggtggg acaagcgcac cggttactcg
      181 atcaatcgcg gagagttgct ttccgacgcg gccttcggcc acggcggctt caccggcacg
      241 gtgctctgga tcgatccgga gctggatctg tttttcattt tcctgagcaa tcgcgtgcat
      301 cccgacggca agggcctggt gaatcccctc gccggccgca tcgccaccgt ggccgccgcg
      361 gcaattcgcg acgtggaaaa acgcactgcc gccggaacgg tcctgaccgg catcgatgtg
      421 ctgaagcgcg acggcttcaa gcagctggcc ggccgcaagc tcggcctgat taccaatcac
      481 accggcaaga gccgcgaagg cgaaaccacg atcaagctgc tccacgaggc gccgaatgtc
      541 gagctaaggg cgctgttcag cccggaacac ggcttcgagg gcaagctcga cgtcagcaaa
      601 atcggcgaca cgcgcgacgc cgccaccggc ttgaaagtct tcagcctgta cggcgagacc
      661 cgcaagccca cgcccgagat gctggccggc atcgacacgc tggtcttcga cattcaagac
      721 atcggcgcgc ggttctatac ctacacctcg accatgggcg aggccctgaa ggcctgtgcc
      781 gagcatggca aacgtttcgt cgtcctcgat cgacccaacc cgatcggcgg caccaaagtc
      841 gccggaccga tgctcgatgc cggcaaggaa tcgttcgtcg gcttccacaa gctgcccgtg
      901 cggcatggca tgacgatcgg ggagttggcc cggctgttcc aggaggagtt gaagctccaa
      961 gtcgatttgc aggtgatcca gtgcgaaggc tggaaacggg cggacgcctt tgacgcgacg
     1021 ggtctgacgt gggttaatcc gtccccgaat atgcgcagcc tgacgcaggc ctttctctat
     1081 ccgggcattg gcctcttgga aacgacgaac ctctccgtgg gccgcggtac cgacacgcct
     1141 tttgaagtca tcggcgcgcc ttggctcgaa gggcagaaac tcgccgccga gctttccgcc
     1201 cgcggcattc ccggcgtcgg cttcgtcccg atcgagttca cgcccaccag cagcaaattc
     1261 gcgaaccaga agtgcggcgg cgtcaacatc ctgatcaccg accgcagccg cttcgagccg
     1321 ctccgcacgg gattcgaaat cgctgcccag ctccgcaagc tctatcccga taagtgggaa
     1381 gccaagggct acgatcggct gttggggaac gaaaagacgt tgcaagcgct cctggaaggc
     1441 aagcccgcag aggaattgga ggcggtgtcg cgggtagggg tggcggagtt tgtgcgtagt
     1501 aggtcgcgcg tgttgattta tgactgaagg agcattcgcc gcgcttcatt tggcgaattc
     1561 ccatttgtcc gctatggttt agcagccgat tcccctgcgg ctccaacctc gtcattgtgg
     1621 gagactcgtc gtgtcacgcc gccggtctgg ccctgcgcgc acattgggcc tggaatcgtt
     1681 ggaaacaagg aatctgctgg caaccttttt tgtcgccaat acgggcaacg acggaaatga
     1741 cggcggttcg gctgcaccct ggctgacgct gcaaaaggcc gccaattcgg tgcaggccgg
     1801 agacacggtc atcgtgcggg ccggcaccta cgcgggcttc gacctcagga ccgacggcac
     1861 ggccgccaat cggatcgtct ttcagggcga ggctggcgcg acgatcactt cgcggaaccc
     1921 gatcaccccc gacggcatca acctcgaggg ggccgactat gtgacgatcg agggtttcaa
     1981 ggtcgtcggc atgccgcgga cgggcatccg ctccgtgctg aaccaccacg tcatcattcg
     2041 caacaacgag tgcgacctga atcagagttg gggcattctc acgggcttca gcgacgacat
     2101 cctcatcgag aacaacatca ccacgcggtc ggtcgttgag cacggcatct acttcagcaa
     2161 cagcggcgac cggccgatca tccgcaacaa cgtctcgtgg ggcaaccggg ccaacggcat
     2221 ccacatgaac ggcgacgttt cgctgggcgg cgacgggatc atcagcaatg ccctggtcga
     2281 aggcaacgtg atctacggca atggcgtggg cggcgggtcg ggaatcaact gcgacggcgt
     2341 gcagaattcc acgatccgca ataacctgat ctacgacacg cacgccagcg gcatctcgct
     2401 ctaccagatc gacggcggcg gcggctcctc gggcaacctc gtggtcaaca acacggtgat
     2461 cgtcgccgcc gacggccgct gggccctcaa catccagagc ggcagcacgg gcaacacggt
     2521 ccgcaacaac atcctctaca ccgctcattc gttccggggc agcatcgaca tcagcgccga
     2581 cagcctggcg ggtttttcga gcgactacaa cgtgctgatg aaccgcctga ccaccaacgg
     2641 cggcgacagc gtccagacgc tcgcccagtg gcaggcttcc agcggccagg acgcgcactc
     2701 gctcgtggcg acgcccagcc agctgttcgt gaacgccgcc ggtttcgact ttcacctctc
     2761 ggcggcgagc gccgcggtcg atgccggcac gactcagttc gccccgctca tcgatttcga
     2821 ggggcacgct cggcccagcg gcagcggcat cgacatcggc gccgacgagc tgagcggcgg
     2881 cccgccgccg cctccgcccc cgccgacgaa tcacgcgccg accaacgtcg cgctcagcgg
     2941 gagcacgatc aaggagactg ccgctgccgg ctcagtggtc ggcacgctct ccgtcgcgga
     3001 tcccgacgcg ggcgacactc acacgctgtt gctggtcaac gacgcgggcg ggcgattcgc
     3061 cctgtcaggc aaccagctcg cccttgcagg tccgatcaac tatgaggccg cggcgagcca
     3121 ttcgatcgta gttcgggcaa ccgacgctgg cggcctgtcg ttcgacaaga cgctggtcgt
     3181 cggcgttacg aacgtcaacg agctagtgcg gatcgacgtg cagaaagggg ccgtgcagcg
     3241 gtcgtacgtc cgctacgtcg atttgatctt cgaaagctcc gcgggcctgt cgcagctcat
     3301 cagcgaaggt cgcatcaact tgacacggtt caccctggcc ggcgcgagcc cggtcaacgt
     3361 cgccctggcc ggcaagctga aggtcagcgg caaccggatc atcgccgact tcgggacgaa
     3421 cggcatcggc ggcaatcgca acaccgccac cggcaacggc tactaccggt tcggcgtcga
     3481 tgccgatcgc aacggcacga aggagacgca gaagcacttc taccgcctgt tcggcgacac
     3541 gaacggcgac cggaccgtca acgccaccga ccaggcgaat gtcaacgccg accgcggcaa
     3601 gaccggcagc aacctccgct ccgatctgaa cggcgacggc ttcgtgaacg cgaccgacct
     3661 gacctacgtc aaggcccagc tcggccgcac gctctggtcg gcgctgccgc ttgacgactg
     3721 atcgcgtctc atcgctcgtt tatcgccctg ctctagcgag ccgcgatcgc tgacgcaagc
     3781 ctttgattcg cctcatgttg cggcgagttc ttgcaccagt ttctgcatgt ccgcaatctt
     3841 gtttgcagca agcgtattca gttcattttg agcgcttgtc aggtttctcc ttcgaccggc
     3901 atatggagat agaaggtctc ctgcgcgagg gattggcaca acgacttcag aaaggactga
     3961 gaaatgcggt ttccaaagct cgttttgcta ctctgcgccg ctgcctgggc gatcacgcca
     4021 caggctcagg tccagggcgg gatattcacc ctgacgaacg cgacctctgg catcttcgac
     4081 gcgagttccg gcacccggtc cgttactgtc accggcttgg aagtgggctt cgatggcggc
     4141 gtcctcacca acatcaccgt cgccattgac tacctcaagg cggacggtga ggctgccgac
     4201 ccgccaccgc cgcctccgct gccgggctca ccattcttca acgagattca ctttcgcctc
     4261 gaccgtgccg ccaatccgag caccgaactg attgcggcgg gccactatgg cgttggtggt
     4321 gccggggact ttttttccgg gattcagacg tttgcgaccg gcggggccat aatcccggca
     4381 ggtggcagcc cggtagccgg gacgttcgcg ccaaccgggc ccgggtcgct tgccgcctac
     4441 aatggcgcct cggctctggg agtctggaca ctctttgtcg aggacacagt cggcctcgac
     4501 gccctgcgat tccgttcggt gacttttacg ttcactaccg agagcgccgc ggtgccggag
     4561 ccgagcagct gcgctattct tggtgccgga gtggtcggac tgctgggctt ttgccgtcgc
     4621 cgccgtcagc tggccgcctg aggcacaagt gaaaacctcg aattgcgggc cgcgagctct
     4681 cccgcgaagc cccggcgctt gacgagtgac ggccgcacgg tacaacggcg gcatgtccaa
     4741 gctcaacggc aaggtggcgc tgatcacggg cgcgggccgc gggatcgggc gcggcatcgc
     4801 cctggccttc gccggcgaag gggcggtact ggttttgacc gcgcggtcgc gcgagcagct
     4861 ggagtccgtc gcccgcgagg cggcgcagct cagcaaagcc ccggtaatcg tgcaaccggc
     4921 ggacgtgtcc gacgagcagc aggtggagtc gctgttcgcg cgcgtgaagg ccgagttcgg
     4981 ccgcctcgac attctcgtca acaacgccgg ggccttcgac ggcgggccgc tcgaccagct
     5041 ttccacggaa gcctgggacc gcgtcatcaa cacgaacctc cgcgggccgt ttctctgcac
     5101 gcgggcggcc ctacggatga tgaagccagt tggcagcggc cggatcatca atatcggctc
     5161 gatcgcggcg cagcgggtac ggccgaattc cgcgccctat gccgccagca agcacggcct
     5221 gtggggcctg acgcaagcga cggcgctgga aggccgcgag tacggcgtga cctgcggggc
     5281 tgtgcatccc ggcaacgtca tggtcgagcg ccgagccggc agcggccgcg gcgaagacga
     5341 cgagccgatg atgacgcccg ccgaaatcgc cgaagtcgcc ctgctgatgg ccacgctgcc
     5401 gccgcacgtg gagctgctgg aggcgattgt gctgccgcac cggcagcact acgtcggacg
     5461 cgggtagttg tcatttgtca cttgccattc gtcatttgta gtccgttctg atggcttctc
     5521 ccattcgact cgcgtttctc ggcatcgatc atccgcacgg cgcgcattgg cggcagcagc
     5581 tggcgaactt taccggcgaa gtcgagatcg tggccttcgt gccgtcgttc ggcggtggaa
     5641 cgaccagcct ggaagagcgc tatgccgagc tgccgcggtt tgaatccgtg tcggcgctgc
     5701 tcaagggggc gcagttcgac gcggcatttg tcggcctggc gaatcgcgat gggccgccgg
     5761 cgatcgtcga gttggccgct gccggcaaac acatcctggc cgagaaaccg gtggcggcta
     5821 gcgccgcgga cgcccggccg atggtcgccg cggtcgaaaa gagtggcgtc gcttatcagg
     5881 cgggctacat gtggcgctac gacgacatcg ccaatcgcct gaagcgaatg ctgcagcagg
     5941 atgcgtttgg caagttaatc agcgtcgaaa tgacctttgc caccagcgac gttgctcgcc
     6001 gcggccccga gcattacttg ttcgatccgg cggccagcgg cgcgggcttt ttcagttggc
     6061 tcgcctgcca ctggctcgac actttgttct tcgtgaccgg ccgcgcggtc gtcggcgtca
     6121 ccgcccgcac gggcgttttc ggcgccgtgc caacgaaagt cgaagacggc ggcgcggcga
     6181 tcctcgagct cgacggcggc ggccttgcga cgttcgttgg cggctactgg catccgcggt
     6241 gggccggcga gagccgctgg gccatccgcg gcagccagcg gtgggtccac tgggacgcca
     6301 atcgcaaagg caccaacgga gcgctcgaaa tccacggccc gcagccgcag tggcatgcga
     6361 tggaagacgt cttttccact cccgaggaca agtcgcccgg ctacggcggc cgccgcggct
     6421 ttttgctcgt gcaggactgg ctcgactgca tccgcacctg ccgccgcgac aaccgggcaa
     6481 ccgcccacgg cacgctggct acgctggagc tgatcgatgc gatcttccag agcagccgcg
     6541 agggcaagcg gatcgaatgc cggatcgagc cggcgtaggg ttattgagcc ctgcatagcc
     6601 aatgtcgtct aa
//
LOCUS       DASRJB010000073         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1150125, whole genome shotgun sequence.
ACCESSION   DASRJB010000073 DASRJB010000000
VERSION     DASRJB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150125"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /gene="infB"
                     /locus_tag="VFB80_02030"
     CDS             <1..1460
                     /gene="infB"
                     /locus_tag="VFB80_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZN32560.1"
                     /translation="NVVAGEAGGITQHIRAYLIETKDGRHVSFVDTPGHEAFTEMRAR
                     GANVTDIAVLVVAADDGIMPQTEEAISHAKAAEVPIVVAMNKIDLPSRDEQKVLQQLA
                     AANLLPSEWGGEVEVVRTSAITGQGIDTLLETLLVTAELHEYKANPNKAARGMCLEAE
                     QEPGRGVIAKLMVQAGTLKEGDVIVCGGGHGRVKAMYDTLRPRQKLKKAGPSTPVNVT
                     GLDMAPQAGDKFYVLEDIAEARALADQRQNAVREQALSGISTKVSFEDFQQRLAEGRL
                     GPDEEAATLNLIIRADVRGSIEAILKELAKFEHPEVKVKVLQASVGGVTVADVTLAQA
                     SDAVIVGFNVIPDEAARALADARHVEIRRYDIIYKLTDDIKALVEGKLKPEERVVELG
                     AALVKQVFVISRVGAVAGCQVIRGAIERGCRIRVNRDSRTIGDYAMESLKREKDDAKE
                     VARGMECGIKLANFNDIKEGDVLEAYKVEEVARTL"
     gene            1555..1947
                     /gene="rbfA"
                     /locus_tag="VFB80_02035"
     CDS             1555..1947
                     /gene="rbfA"
                     /locus_tag="VFB80_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZN32561.1"
                     /translation="MSRRLLKAASAIREVVSMAILTELRDPRVKNVTVVGVEVLPDMK
                     SAKVLVSIMGDEKQQQLSLSGLQNSAGFLQKRIAERIETRYTPRLTFVLDKGVKNSLE
                     VARILHEVLPPKPVEDGGEEQDSQQEGE"
     gene            2056..3018
                     /locus_tag="VFB80_02040"
     CDS             2056..3018
                     /locus_tag="VFB80_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32562.1"
                     /translation="MTTNRTALISKVYKVLKKHYKPSAPPDDRTVLEHLLYACVLENA
                     RYEAADEAFAKLKELYFDWNEIRVTTVTELAEGMTTLPDASQAAQRVKKALQSVFETG
                     YSFDLEALKKQNIGKSEKDLEKISGTTPFVRSYVTQNALSGHSIPVSKGALDVLYAVG
                     IVTDAEADKGQAPGLERAIPKNKGVEFGSLLQQAGADLVAAPGSTKLKAALAEMDGQY
                     KERLAQRQARLAEQAAADAAHAKEARDKARAEARLAAAEARPQPKLAPKEAPRLPAPS
                     SAAEAKAREQDKAKPTAPPARKKEDAAKPLRPSAAKGLAKKKPR"
     gene            3064..5319
                     /locus_tag="VFB80_02045"
     CDS             3064..5319
                     /locus_tag="VFB80_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN32563.1"
                     /translation="MTRPALCLLALLWPATRLPAQEQATDAAPRANPLAGVEKLPPEA
                     TRGEADDDRVTFTVHLLQARLAQGRGERAEALRHYQRAWRYAPEQTSLLREIVALAFA
                     LKRNDEALRYAALSAQAEGADPLLLRRLALVATSRRQWAQAVKLFESGQRLDEKARQP
                     GEKLDQAALTVKLEMGRLYFLAGDYERSAASFAVVRAALADPQSGLSDEARQMLLDKP
                     AETYALWAESFLAAGKTDDAEALFRQADEAMSDPATLAFQLARVEAARGNPSSARREL
                     EEYFATKSTSSGIEPYNLFAEMVAKHQPDPVQARKTVIDRFEKRAAQDPENLPLKLSL
                     AGQYLAANQLDKAEPLLVAVVKVMPTAAAYEKLTEIYHRQNKFDKLLAIAAEVAKRTT
                     SLDLLGEAGQKVAASKGTMQRLIDGVRLRRRTRPDSLAPDEVLAVGLLALAAKEYNAA
                     DELLAAAAEQQPEKAETILLRWGFGMMLADQYPRAASAFGKLIDRQPPDEQLAQGYYY
                     LAGALELAGQTDAALAAAKSGAEKRPDDPRFAGRAAWVLYHAKRTSEAKAAYAEFVAK
                     FDKLPEETAVRETAREARLVLSNLENVAGDFNAATEWLEQVLDEFPEDVGASNDLGYL
                     WADKNAHLQRAQRLIELAVNAEPENHAYRDSLGWVYFRQGKFAEAVVELEKAAANEKA
                     DGVILEHLGDALGKAGRTSEARAAWLRAEAAFQKDRAADKLKRVREKLEEPKNQAGKE
                     PKNQRTEEPKK"
     gene            5364..>5838
                     /locus_tag="VFB80_02050"
     CDS             5364..>5838
                     /locus_tag="VFB80_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN32564.1"
                     /translation="MAGHSPWANIKHRKAAVDKKRAKLWTKLSKAIIVAAQMGGGDPD
                     SNLRLRYAIADAKAVSMPKDNIERAIKRGTGELEGGKVEEVLYEGYGPGGAAILCEIM
                     TDNRNRTAPEIRKVFETHDGKLGATNCVAWMFERKGVFLIAADKVSEEKLMEVVLE"
BASE COUNT         1210 a         1854 c         1937 g          837 t
ORIGIN      
        1 ccaacgtcgt ggccggcgaa gcaggcggca tcacgcagca catccgggcc tacctgatcg
       61 agaccaagga cggccggcat gtatcgttcg tcgatacgcc gggccacgaa gccttcaccg
      121 agatgcgggc ccgcggagcc aacgtcacgg acatcgccgt gctggtcgtc gccgccgacg
      181 acggcatcat gccccaaacg gaagaggcga tcagccacgc caaggccgct gaagtgccga
      241 tcgtcgtggc gatgaacaag atcgacctgc cgtcgcggga cgagcagaaa gtcctgcagc
      301 agctggcggc ggcgaacctg ctgcccagcg agtggggcgg cgaagtcgaa gtcgtgcgga
      361 ccagcgccat caccggccag gggatcgaca cgctgctgga aacgctgctg gtcaccgccg
      421 aactgcacga gtacaaggcc aacccgaaca aggcggcccg cggcatgtgc ctcgaagccg
      481 agcaggagcc cgggcgcggc gtgatcgcca agctgatggt gcaggccggc acgctgaaag
      541 aaggcgacgt gatcgtctgc ggcggcggcc acggccgcgt caaggcgatg tacgatacgc
      601 ttcgcccgcg gcagaagctc aaaaaggccg ggccgagcac gccggtcaac gtcaccggcc
      661 tcgacatggc tccgcaggcc ggcgacaagt tctacgtgct ggaagacatc gccgaggccc
      721 gggccctggc cgatcagcgg caaaacgccg tccgcgagca ggccctctcg ggcatctcga
      781 ccaaagtctc gttcgaagac ttccagcagc ggctggccga aggccgcctg ggcccggacg
      841 aagaggccgc caccctcaac ttgatcatcc gggccgatgt ccgcggctcc atcgaggcga
      901 tcctcaagga gctggcgaag ttcgagcacc cggaagtcaa ggtcaaggtg ctccaggcct
      961 cggtgggcgg cgtgaccgtc gccgatgtga cgctggctca ggccagcgac gcggtcatcg
     1021 tgggcttcaa tgtcattccc gatgaagccg cccgcgcgct ggccgacgcg cggcacgtcg
     1081 aaatccgccg ctacgacatc atctacaagc tgaccgacga cattaaggcc ctcgtcgaag
     1141 gcaagctcaa gcccgaagag cgggtcgtcg agctcggcgc cgccctggtc aagcaggtct
     1201 tcgtcatcag ccgcgttggc gccgtcgccg gctgccaggt catccgcggc gcgatcgaac
     1261 gcggttgccg gatccgcgtc aaccgcgaca gccgcacgat cggcgactac gcgatggagt
     1321 cgctgaagcg tgaaaaggac gacgcgaagg aggtcgcccg cggcatggaa tgcggcatca
     1381 agctggcgaa cttcaacgac atcaaggaag gcgatgtcct cgaagcgtat aaggtcgagg
     1441 aagtggcccg cacgttgtag agatgtcatc tgtcacttgt cactggtcat ttgaagtgca
     1501 gcccgcgatt caaatgacaa gggacgagtg accaatgaca accgaccagc acccatgtcg
     1561 cgccgattac tgaaagccgc ttccgccatc cgcgaggtcg tcagcatggc gatcttgacg
     1621 gagctgcgcg atccgcgggt gaagaacgtg acggtcgtgg gcgtggaagt gctgccggac
     1681 atgaagtcgg ccaaggtgct ggtctcgatc atgggggacg agaagcagca gcagctcagc
     1741 ctgtcggggc tgcagaattc cgccggcttc ctgcagaagc ggatcgctga gagaatcgag
     1801 acccgctaca cgccgcggct gacgtttgtg ctggataagg gcgtgaagaa ctcgctggaa
     1861 gtggccagaa ttttgcacga agtgctgccg ccgaagccgg tggaagatgg cggcgaagaa
     1921 caagactcgc aacaagaggg agaatagccg tcatttgtca gcggtcattg gtcactggtc
     1981 actagtcatt tgagttagtc gctcaaatga ccaatgacca atgaccaatg actagtgacc
     2041 agtgacaaat gaccaatgac aacgaaccgc accgccctca tctccaaagt ctacaaagtg
     2101 ctgaagaagc actataagcc cagcgcgccg cccgacgacc ggacggtgct ggagcacttg
     2161 ctgtatgcct gcgtgctgga aaatgcgcgg tacgaggcgg ccgacgaggc ctttgccaag
     2221 ctcaaggaac tttacttcga ctggaacgaa atccgcgtga cgaccgtgac ggagttggcc
     2281 gagggaatga ccaccctgcc cgacgcctcg caggccgccc agcgggtgaa gaaggcgctc
     2341 cagtcggtgt tcgagacggg atattcgttc gacctggagg cgctcaagaa gcagaacatc
     2401 ggcaagtccg aaaaggacct ggagaagatc agcggcacga cgcccttcgt gcggtcgtac
     2461 gtgacgcaga acgccctctc cgggcacagc ataccggtca gcaagggcgc gctcgacgtg
     2521 ctctacgccg tgggaatcgt gaccgacgcc gaggccgaca aggggcaagc cccgggcctg
     2581 gagcgggcca tccccaagaa caagggcgtc gagtttggct cgctcttgca gcaggccggg
     2641 gccgatctcg ttgccgcccc aggttccacg aagctcaagg ccgccttggc cgagatggac
     2701 ggtcagtaca aggagcggct ggcccagcgg caggcccgcc tggccgagca ggccgcggcc
     2761 gacgcggccc acgccaagga agcccgcgac aaggcccgcg ccgaggcccg cctggccgcg
     2821 gccgaagccc ggccgcagcc gaagctcgcg ccgaaagaag ctcccaggct tcccgctccc
     2881 tcttcggcgg ctgaggccaa ggccagagag caagacaagg cgaaacccac cgctccgccg
     2941 gctcggaaga aagaggatgc cgcgaagccg ttgcggccgt cggccgccaa aggcctggca
     3001 aaaaagaagc cgcgctaggg cccgcctcgc ggtagaatcg agcgctgggg acggaggagt
     3061 tccatgacac gtcccgcgct ctgtttgctg gcgttgcttt ggccggcgac tcgtttgcct
     3121 gcgcaagagc aggccacgga cgccgctccg cgcgccaatc cgctagccgg cgtcgagaag
     3181 ctgccgcccg aggccacccg cggcgaagcc gacgacgacc gcgtcacgtt caccgtgcac
     3241 ttgctgcagg cccggctggc ccagggccgc ggcgagcggg ccgaggcgct gcggcactat
     3301 cagcgggcct ggcgttatgc ccccgagcaa acttcgctgc tgcgcgagat cgtcgcgctc
     3361 gcattcgccc tgaagcggaa cgacgaggcg ctgcgctatg ccgccctgtc cgcccaggct
     3421 gagggagccg atccgctcct gctccgccgg ctggccctgg tcgcgaccag ccgccgccag
     3481 tgggcccagg ccgtgaagct cttcgaaagc ggccagcggc tggacgagaa ggcgcggcag
     3541 ccgggtgaga agctcgatca ggccgcactc accgtcaagc tggaaatggg ccggctctac
     3601 tttctggccg gagactatga aaggtcggcc gcgtcgtttg ccgtcgtccg cgccgcgttg
     3661 gccgatccgc agtcgggcct gtcggacgag gcccgccaga tgctgctcga taagccggcg
     3721 gagacatacg cgctatgggc cgaatcgttc ctcgccgccg gcaagaccga cgacgccgaa
     3781 gccctcttcc gccaggccga cgaggccatg agcgatccgg cgacgcttgc ttttcagctg
     3841 gctcgcgtcg aggccgcccg cggcaatccc tcgtccgccc gccgcgagct cgaagagtac
     3901 ttcgcgacga aatcgacttc gagcggcatc gagccgtaca acctgttcgc cgagatggtc
     3961 gccaaacacc agcccgatcc ggtccaggcc cgcaagacgg tgatcgacag gttcgagaag
     4021 cgggccgccc aggatccgga aaacctgcca ctcaaattga gcctggccgg tcaatacctg
     4081 gcggccaatc agctcgacaa ggccgagccg ctgctagtcg ccgttgtcaa agtcatgccg
     4141 accgccgcgg cctatgaaaa gctgaccgag atctaccacc gccagaacaa gttcgacaag
     4201 ctgctggcga tcgctgccga ggtcgccaag cgcacgacgt cgctcgacct gctgggcgaa
     4261 gccggccaga aagtcgccgc cagcaagggc acaatgcaac gattgatcga tggcgtccgc
     4321 ctgcggcggc gcacgcggcc cgattcgctc gcgccggatg aagtgctggc agtgggcctg
     4381 ttagcgctgg ccgcgaagga gtacaacgcg gccgatgaat tgctggccgc cgcggccgag
     4441 cagcagccgg agaaggccga gacgattctg cttcggtggg gttttgggat gatgctcgcg
     4501 gaccaatatc cgcgggcggc ttcggcgttt gggaagctga tcgaccgcca gccgccggat
     4561 gagcagctgg cgcagggtta ttactacctg gccggcgccc tggagttggc gggccagacc
     4621 gacgcggcgc tggccgccgc gaaaagcgga gccgagaagc gcccggacga tccgcgcttc
     4681 gcgggccgtg cggcatgggt cttgtatcac gccaagcgca ccagcgaggc caaggctgct
     4741 tatgccgagt tcgtggcaaa gttcgacaaa ttgccggaag aaaccgccgt tcgcgagacg
     4801 gcccgcgaag cccggttagt gctctccaac ctggaaaatg tggctggcga tttcaatgcg
     4861 gcgacggagt ggctcgagca ggtgctcgac gaattccccg aggatgtcgg cgcgagcaac
     4921 gacctgggct acctctgggc cgacaagaac gcacatctgc aacgtgccca gcggctgatc
     4981 gagctggcgg tgaacgccga gccggaaaac cacgcctatc gcgacagcct gggctgggtc
     5041 tacttccggc aagggaagtt tgccgaagcc gtcgtcgagc tcgaaaaggc cgccgccaat
     5101 gagaaggccg acggcgtgat cctggagcac ctgggcgacg ccttgggcaa ggccggccgc
     5161 accagcgaag cccgcgccgc ctggctgcgg gccgaggcgg cattccagaa agaccgcgcc
     5221 gcggataagt tgaaacgggt cagggaaaaa ctcgaagaac caaagaacca agcaggcaaa
     5281 gaaccaaaga atcaaagaac cgaagaacca aagaagtaga actgttcttc ggttcttcgg
     5341 atcttggaat ttgaaccccc cctatggccg gccactcccc ttgggcgaac atcaagcacc
     5401 gtaaggccgc cgtcgataag aagcgggcca agctctggac caagctatcc aaggccatca
     5461 tcgtcgcggc ccagatgggg ggcggcgatc ccgattcgaa cttgcggctg cggtatgcga
     5521 tcgccgacgc caaagcggtg agcatgccca aggacaacat cgagcgggcg atcaagcgcg
     5581 gcacgggcga attggaaggc ggcaaagtcg aggaggtcct gtacgaaggc tatggtcccg
     5641 gcggcgcggc gatcctgtgc gagatcatga ccgacaaccg caaccgcacg gccccggaaa
     5701 tccgcaaggt ctttgagacc cacgacggca agctcggtgc gaccaattgt gtcgcctgga
     5761 tgttcgagcg gaagggcgta ttcctgatcg ccgccgacaa agtcagtgag gagaagctga
     5821 tggaggttgt gctggagg
//
LOCUS       DASRJB010000074         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2562116, whole genome shotgun sequence.
ACCESSION   DASRJB010000074 DASRJB010000000
VERSION     DASRJB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562116"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="pgk"
                     /locus_tag="VFB80_02055"
     CDS             <1..1305
                     /gene="pgk"
                     /locus_tag="VFB80_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZN32565.1"
                     /translation="IPRLDSLANVARGTPVLVRGDVDCKPGPEVGQEDIRLRSMKETL
                     EFGRRQGWKQIVFGHLGRKQPDKPIGSLAKVAARLGQILGCDVPLVEDWLDEASGTIK
                     PQVTQKVAAAAAGGVLMLQNVRAYDIETVLWKAKENALPQIAAKLAAMANSLAEKVAG
                     VYVNEALSAGSLDASSTIVPLAMQRVALGAYVAREFEGPMQDCLQAELVVFSGIKIDK
                     LDDLEAMIARGTIRTIISAGSLAMSLKKAAALLAGTNFSLGVSEDAGHKDKPYYIPPE
                     RIEQARRMVAEAQRLGIELVLPVDFKLGDGRVVETLAPGDQQFDIGPKTISHFEKKIG
                     EFIAAHRGKPAVVFHNGVFGMFEDPRFEEGTKAFIQQLKRLKDAGMRVYIGGGEGGTA
                     LEKYGRPEWVTHCFTAGGTVLNALGAEPVPYLVALRAAVKGL"
     gene            1623..2105
                     /locus_tag="VFB80_02060"
     CDS             1623..2105
                     /locus_tag="VFB80_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32566.1"
                     /translation="MKSRLLAAVIAAGLFCLCAQANAFEFLRMMSGGYGCGCDNVCGC
                     DHHAKCGCDNVCGCDRGCRSHCHHRNRCGCGCDNVCGHDKGCGCAAPTCAAAAPTCAA
                     APSCGCETKCGCDRGCHRDRCCKDRCHRDRCCKDRCHHHRGCGCDNVCGHDKGCGCGG
                     "
     gene            complement(2230..>2944)
                     /locus_tag="VFB80_02065"
     CDS             complement(2230..>2944)
                     /locus_tag="VFB80_02065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN32567.1"
                     /translation="TLKEATLAQAQRPLEFEPGSKWAYCNAGIDTLGRIIEAKSGRPY
                     EDFLRDRVFQPLDMEDTSFYPSEEQLSRLAGLYGQKDGQLVFADYQLIGPTKDARHPI
                     PAGGLFSTGSDLAALYQAMLHRGWHGVTKAGDKRLLSEAAFRQMTQLQTGDLQCGFTP
                     GMGFGFGWAVIKEPQGVHAMLSKGTFGHGGAFGTQGWIDPAQDLFVILLIQRTGLANS
                     DASDLRKELQRLAVAAIRR"
BASE COUNT          500 a          959 c          989 g          496 t
ORIGIN      
        1 attccgcggc tggactcgct ggcgaatgtc gctcgcggca cgccggtgct ggtccgcggc
       61 gacgtcgatt gcaagccggg cccggaagtt ggccaggaag acatccgctt gcggtcgatg
      121 aaggagacgc tcgaattcgg ccggcggcaa ggctggaagc agatcgtctt cggccacctc
      181 ggccgcaagc agccggacaa gccgatcggc tcgctggcca aagtcgcggc ccggctgggg
      241 cagatcctgg gctgcgacgt gccgctagtc gaggactggc tcgacgaagc cagcggcacg
      301 atcaaaccgc aggtgacgca gaaggtcgcc gccgcagcag ccggcggcgt gctgatgctg
      361 cagaacgtgc gggcgtacga tatcgagacc gtcctctgga aggccaaaga gaacgcgctg
      421 ccccagatcg cggcgaaact ggcggctatg gccaattcgc tggccgagaa agtcgcgggc
      481 gtgtacgtga acgaagccct ctcggccggc agcctcgatg cctccagcac gatcgtgccc
      541 ctggccatgc agcgggtcgc tttgggggcc tacgtcgccc gcgaattcga aggcccgatg
      601 caggactgcc tgcaggcgga gctggttgtc ttcagcggca tcaagatcga caagctcgac
      661 gacctggagg cgatgatcgc ccgcggcacg atccgcacca tcatcagcgc cggctcgctg
      721 gccatgtcgc tgaagaaggc cgcggcgctg ctggccggca cgaacttctc gctgggggta
      781 agcgaagacg cggggcataa ggacaagccc tattacattc cgcccgagcg gatcgagcag
      841 gcccgccgga tggtggccga agcccagcgc ctcggcatcg agctggtgct gccggtcgat
      901 ttcaagctgg gggacggccg ggtggtcgag acgctcgccc cgggcgatca gcaattcgac
      961 atcgggccca agaccattag tcatttcgaa aagaaaatcg gcgagtttat tgcggcccac
     1021 cgcgggaagc cggcggttgt cttccacaac ggcgtcttcg gcatgttcga ggatccgcgg
     1081 ttcgaggagg gaaccaaggc gtttatccag cagctcaagc gcctgaagga cgcagggatg
     1141 cgggtctaca tcggcggcgg cgaaggcggg acagccctcg agaagtacgg ccggcccgag
     1201 tgggtcaccc actgcttcac cgccggcggc accgtgctga acgccctcgg ggccgagccc
     1261 gtgccctacc tggtcgccct gcgagcggcg gtgaaggggt tgtagcaact gcctgcctgc
     1321 gatttggtcg tttacaggcc gcattcctgg cacgcccggc aaacctcgtg caagcggccc
     1381 ggcaagctgg caaaaatccg catgaccctg ccgaccagtc aagtgcggca aattgagcag
     1441 acgatagcac tgcgtagatt cagccgggac tgcgggagtg cagcccgctg attcgtcgct
     1501 gaacatcctt gatacggctg ccgcaatgtg ccgagtgcca cgcggggccg gcggttccgg
     1561 cgacacgtgt ccgttgtttc cgtttccgtc acccttcgtg tttggaaaga ggagtctctc
     1621 caatgaagag ccgtctgttg gccgccgtca tcgcggccgg tttgttctgt ctgtgtgcgc
     1681 aagcgaatgc gtttgagttt ctgcgaatga tgtccggtgg ttatggctgc ggctgcgaca
     1741 acgtctgcgg ctgcgaccac cacgccaagt gcggctgcga caacgtctgc ggctgcgacc
     1801 gcggctgccg ctcgcactgc catcatcgca atcgttgcgg ctgcggctgc gacaacgttt
     1861 gcggtcacga caaaggctgc ggctgcgccg ccccgacttg cgccgccgct gctccgacct
     1921 gcgccgccgc tccgagctgc ggttgcgaga ccaagtgcgg ttgcgaccgc ggctgccacc
     1981 gcgaccgttg ctgcaaggac cgctgccacc gcgaccgctg ctgcaaggac cgctgccatc
     2041 accaccgggg ctgcggctgc gacaacgtct gcggccacga caaaggctgc gggtgcggcg
     2101 ggtaatcgac tgccattagc tcggggcctc cggccgcata cgcctctgcg gacggaatgc
     2161 acccgagaac gagatacggg cgcagcctcc ggccgatcac cggaggcttc gctgctttct
     2221 tgaggcgaat cagcggcgaa tcgctgccac cgccagccgc tgcaattcct tgcggaggtc
     2281 gctggcgtcg ctgttcgcca ggcctgtccg ctggatgagc aggatcacga acaggtcctg
     2341 cgccggatcg atccagcctt gcgtgccgaa cgcgccgccg tggccgaacg tccccttgct
     2401 cagcatcgcg tgcacgccct gcggctcctt gatgacggcc cagccgaagc caaagcccat
     2461 gcccggcgtg aagccgcatt gcaggtcgcc ggtctgcagc tgcgtcatct gccggaaggc
     2521 ggcctcactg aggagccgct tgtccccggc cttggtgacg ccgtgccacc cgcgatggag
     2581 catcgcctgg tagagcgcgg ccagatcgct gccggtcgaa aacaggccgc cggcggggat
     2641 tggatgccgg gcgtcctttg tcgggccgat cagctggtaa tcggcgaaaa ccagctggcc
     2701 atccttctgc ccgtacaggc cggctaaccg ggacagttgc tcttcgctcg gataaaacga
     2761 cgtgtcctcc atgtcgagcg gctggaacac gcggtcgcgg aggaaatctt cataaggccg
     2821 gccgctcttc gcctcgatga tccggccgag cgtgtcgatg ccggcgttgc agtaggccca
     2881 tttgctgccc ggctcgaact ccagcggccg ctgggcctgg gccagcgtgg cctccttcag
     2941 cgtg
//
LOCUS       DASRJB010000075         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2280238, whole genome shotgun sequence.
ACCESSION   DASRJB010000075 DASRJB010000000
VERSION     DASRJB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280238"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1264
                     /locus_tag="VFB80_02070"
     CDS             359..1264
                     /locus_tag="VFB80_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32568.1"
                     /translation="MTFLQELQRRWRDSTIAGRFVFALLVVVVLGFVSMAWAPAAWAL
                     AALASGALLGFLFAIPRVVQSDAAGQEPYRQKVNSNLEEISDWLTKIIVGIGLFELSQ
                     VPPRFESLARFLVGGTKYTPAFAGTVLVLFSAVGFLLFYLLTRLYLSRAFSEADRAAN
                     DFNRQVAEALAPAARESIGGLESMATGAGDWLEASPKQAVLDAWDRLQNAAQLAIARR
                     APSQSVPSGELQLAQKLVELQLADQTQARTFLQLFKLRNTAAQAIAAGLSQAAVREYV
                     QNAAALAAHLESASRSPNTGSTSGT"
     gene            complement(1739..2977)
                     /locus_tag="VFB80_02075"
     CDS             complement(1739..2977)
                     /locus_tag="VFB80_02075"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZN32569.1"
                     /translation="MDVAAIRRDFPILETTLDDGWPLVYLDNAASTQRPKQVIDAIVE
                     AYEHYYSNVHRGGHALAAESTVRYEAAREAVRALVNARSTTEIVFTPGTTAGINLVAR
                     SWGDANIKAGDEIVLTLMEHHSNIVPWQQLAQRTGCTIRWTPITDDYQLDLDAFQKLL
                     SPRTKLVAVTAISNVLGTINPLEKIIPLAHAAGAVVLVDAAQAAPHEPLDVRAWNADF
                     VAFSGHKMMGPSGVGVLYGREDLLDRMQPFLGGGSMIKQVTTAGFTPADLPYRFEAGT
                     PVIVPAIGLGKAIRYLQKLSMESVRAHELALTRQAHSLLADIPGLRILGPEPERKGGI
                     VTFAIEGIHPDDISKVLDVQGIAVRAGHHCAMPLHERLGVSASTRASFYLYNTPEEVA
                     RLAAGLREGVRILGRKSGEA"
     gene            complement(3158..3547)
                     /locus_tag="VFB80_02080"
                     /pseudo
     CDS             complement(3158..3547)
                     /locus_tag="VFB80_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
     gene            3721..>4010
                     /locus_tag="VFB80_02085"
     CDS             3721..>4010
                     /locus_tag="VFB80_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32570.1"
                     /translation="MLTRAVIGLIAAISLAVPLAAQETAKEFSGPQAGEKVTPFKFRQ
                     VLGDAVGKEIDLVEAAGGKPLLLLFVHEVNRPSVGLARLIGDYAATRKADGLT"
BASE COUNT          659 a         1288 c         1359 g          704 t
ORIGIN      
        1 ctccagcggt tccagcagca accggcgctg gcggcgggca cgggacgaac gcaacgcgga
       61 cgcggggcgc gagggcatgg ggaggctccg ggcggcagca ctccctcgcc catccgcgga
      121 aacaccagtt gtggatcatg agccgccggc ggcttgggcg gccgcggcag cgggcggatt
      181 gtaaccagcg ccggcccgaa ttgccactag cggcctggaa ttcgatgcgc ctcaagaatc
      241 tgcaccggca cagggcacgg aaaaggctgt atcgcgaaac tctgcaccat ggagcgttgc
      301 actggtcagg cgaaaatgat ggacacgcga tgggcggcga agcacaatca ttttgcccat
      361 gactttcctt caagagctgc agcggcggtg gcgcgattcg acgatcgcag ggcggttcgt
      421 ctttgcgctc ctcgtcgtcg tggtcctggg gtttgtgtcg atggcttggg cgccggcggc
      481 ctgggccttg gccgcactcg cctcgggcgc actgctcggc ttcctgttcg cgattccacg
      541 cgtcgtgcag tccgacgcgg ccggccagga accctaccgg caaaaggtga actccaacct
      601 ggaggagatt tcggactggt tgacgaaaat catcgtcggc attgggttgt ttgagctgtc
      661 gcaagtgcct ccgagattcg agtcgctggc ccgatttctg gtggggggca ccaagtacac
      721 cccggccttc gcgggcacgg tcctggtctt gttttctgca gtcgggttct tgctcttcta
      781 tctcttgacc cgcctgtatt tgtcgcgggc cttcagcgag gcggaccggg cggccaacga
      841 tttcaatcgg caggttgccg aggcgctcgc gccggccgct cgcgaatcga tagggggcct
      901 ggaatcgatg gcgactggcg caggcgactg gttggaagcc tcgcccaagc aggcggtcct
      961 cgacgcttgg gatcgactgc agaacgcggc tcagctcgcc attgcgcggc gggccccctc
     1021 tcaaagcgtc cccagcggag agctccagct tgcccaaaag ctcgtcgagc tccagcttgc
     1081 cgaccagact caagcccgca ccttcctgca gctgttcaag ctgcgaaata ccgccgccca
     1141 agccatcgca gcgggacttt cccaagccgc tgtccgcgag tacgtgcaaa acgcggccgc
     1201 cctggcggcg cacctggagt cggcatcgcg cagcccaaat accggctcga catccggaac
     1261 ctaagcccgg ccgaacggct ttgaagctcc gcttgcgaac cagccgcgac ttcgcgacgg
     1321 tttcgtacgc cgccgtgggc aggctcaaga atctctgaaa aatgcgggaa accgcacggt
     1381 actttctatg tggacgggtg tttataatga ggcggcggtg accgccgtgg ggttggtggt
     1441 ggaagttcgc aagggcgaaa tgtacgcctg tgcttataat ggcgcgccgg ttaggataat
     1501 tccgcgaagg gttctctagt gcttctatcg ctcgccgcat tgccgcgctt tcgcgcggcg
     1561 caagtcgcgg cgcggcagaa ggttgtgtca gggcagcagg agatggggcc gtgaaaaatc
     1621 gccagcccga tgggaattat ccttacctcg ccgcgcaaat gcttgcggcg tcttgtgcgg
     1681 cattgccgtt ggtaattcga gtggcaagcc gggagcggcc agaggccccg ccgcaccgtc
     1741 acgcctcgcc gctcttgcgg ccgaggatcc gcacaccctc gcgcaggccc gcggccagcc
     1801 gggcgacctc ttccggcgtg ttgtagagat agaagctcgc ccgggtgctg gccgaaacgc
     1861 ccagccgctc gtgcaacggc atcgcacagt ggtgcccggc ccggacggcg atcccctgca
     1921 cgtcgagcac tttggagatg tcgtcgggat ggatgccctc gatcgcgaac gtgacaatcc
     1981 cgcccttccg ctcgggctcc gggccgagaa tgcggagccc gggaatatcg gccagcaggc
     2041 tgtgggcctg ccgcgtcagg gccagctcgt gggcccgcac gctttccatg ctgagctttt
     2101 ggagatatcg aatcgccttg cccagtccga tcgccggcac aatcaccggc gtgccggcct
     2161 cgaaccggta gggcaggtcg gccggcgtga agcccgcggt cgtgacctgc ttgatcatgc
     2221 tgccgccgcc cagaaacggc tgcatccggt cgagcaggtc ctcgcggccg tagagcacgc
     2281 cgacgcccga aggacccatc attttatgcc cgctgaacgc cacgaagtcc gcattccagg
     2341 cccgcacgtc cagcggctcg tgcggcgcgg cctgggcggc atcgaccagc acgaccgcgc
     2401 ccgcggcgtg ggccagcggg atgattttct cgagcgggtt gatcgtgccc agcacgttcg
     2461 agatggcggt gaccgcgacc agtttcgtcc gcggagaaag cagcttttga aaggcgtcca
     2521 ggtcgagctg gtaatcgtcg gtgatcggcg tccagcgaat cgtgcagccg gtgcgctggg
     2581 cgagttgctg ccagggcacg atgttgctgt ggtgctccat cagcgtgagc acgatctcgt
     2641 cgccggcttt gatgttcgcg tcgccccagc tgcgggcgac gaggttgatc cccgcggtgg
     2701 tgcccggcgt gaaaacgatc tccgtcgtgc tgcgggcgtt gacgagcgcc cgcacggcct
     2761 cgcgggccgc ttcgtaacgc acggtactct cggctgccag ggcgtgcccg ccgcgatgca
     2821 cgttgctgta gtaatgttcg taggcctcga cgatcgcgtc gatcacctgc ttgggccgct
     2881 gcgtgctggc agcgttgtcg agatagacca gcggccagcc gtcatcgagc gtcgtctcca
     2941 ggatcggaaa atcccggcgg atcgccgcca catccagggg cgaggcagcc gccggctgcc
     3001 cggtgatcac ggtcgccggc agggcgggcc gcggcttgcc gttggcgccg ccggccgggc
     3061 gctcgcggac tttgaacggc accagcggca gcgacgtcag agccggcgtc gcgcttccct
     3121 gctgatcggc ggccaggcgg gccagcgtct gaatgcgctg caccatcgaa tacaggccgt
     3181 tgctccgcat cggcgtgatg aactgcttga gcttcagcgt ctgaaacagc tcctcgatcg
     3241 gaaaggcgag aatctcctgc ggggtgcggc ccgagtaggc cgccagcagg atggcaatca
     3301 gcccgcggac catcggcgcg tcgctgtcgg cggtgaagtc gagcgtcggc ggatcgccgg
     3361 ggacctgctc cgccaccagc cagaccatgc tctggcagcc gagcacgcgg ttttcctcgg
     3421 tcttgaggtg cggcaccagc ggcggcaggg tctggccgat ctcgaccaga taggtcatcc
     3481 ggtcgttggg gtcgtcgagc tcggcgaact cggcgcggat ctcgtgcagc ggaacggcaa
     3541 gcggcatgat ggtcgcaatc ggaggacgcc ccggccgcga aaggttcgca ggcgaggcca
     3601 catacctatt ataagcgcca gtccgccccg cgtctggaac gagcaaatcg caacagggcg
     3661 gcggccgtgc tatgatcggc ccatcctgcc gctgaccagt tcctgcctgg agaactatcg
     3721 atgttgactc gcgccgtgat tggactgatt gccgccatta gcctggctgt gccgcttgcg
     3781 gcccaggaaa ccgccaagga attctccggc ccgcaggccg gcgagaaagt cacgcccttc
     3841 aagttccggc aggtgctggg cgacgccgtg ggcaaggaaa tcgatctggt ggaagcggcc
     3901 ggcggcaagc cgctgctgtt gctcttcgtc cacgaggtga accgcccctc ggtcggcctg
     3961 gcccggctga ttggcgacta cgcggcgacc cgaaaggccg acgggctgac
//
LOCUS       DASRJB010000076        10230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3973238, whole genome shotgun sequence.
ACCESSION   DASRJB010000076 DASRJB010000000
VERSION     DASRJB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973238"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFB80_02090"
     CDS             <1..548
                     /locus_tag="VFB80_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN32571.1"
                     /translation="RSQPAAVSRRRPCRPRTERDRRRMKLDSLPVTCEATIPESYLDV
                     MGHMNVMWYTHLFGKAAVGLFRLAGMDRQYFLDHRAGTFALAQVFRYRKEVRVGEHVA
                     IRSRLIARSERQFHVMHFMTKGDEQILAATGEFLAAHIDMQTRRTAPFAPHIAQNLDR
                     LLAEHSALDWAAPVSGAIEVR"
     gene            558..1769
                     /locus_tag="VFB80_02095"
     CDS             558..1769
                     /locus_tag="VFB80_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN32572.1"
                     /translation="MFPEPSPRATELRQRLVAFMDEHIYPSEAQLGAHDQSADRWQPP
                     QLLEQLKAEAKAAGLWNLFLPDRRHGAGLTNLEYAPLCEIMGRSPIAPEVFNCSAPDT
                     GNMEVLARYGTPEQQERWLAPLLAGEIRSCFAMSEPDVASSDATNIRSRIERRGDEYV
                     INGRKWWTSGAGDPRCQLCIFMGQTDPAAAVHAQQSMILVPMDAPGVKVQRMLTVFGY
                     DHAPHGHAEIVFDNVRVPAANILLGEGRGFEIAQGRLGPGRIHHCMRLIGVAERSLEA
                     MCRRVLTRVAFGKPLAEQGTIRAEIADSRIEIEQARLLTLKAAHLMDTVGNKEARAEI
                     AMIKVACPNMALRVIDRAIQAHGGAGVSADFGLAAAWAMTRALRLADGPDEVHREAIA
                     KLELKRHKSQI"
     gene            1796..2557
                     /locus_tag="VFB80_02100"
     CDS             1796..2557
                     /locus_tag="VFB80_02100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN32573.1"
                     /translation="MSVSISLTGKVALVTGGGRGIGKAITQHLAAAGANVVIASRKLS
                     NLQAAAEELSSLPGKVVPIECHVGRLDQLQSLVRETEARLGPVEILVNNSATNIGQGP
                     SLDVTDEMLDKMIEINVKAALRLVRLTVPKMLERQSGAIINIASIAGLHPQPQSLLYS
                     FTKAGLIMMTRSWAREFGPQGVRVNAIAPGLIQTDFSEYYWKDDERRRQIESRQPIAR
                     IGQPEEIGHLALYLASSEASYVTGQVFVVDGGATA"
     gene            complement(2567..3472)
                     /locus_tag="VFB80_02105"
     CDS             complement(2567..3472)
                     /locus_tag="VFB80_02105"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN32574.1"
                     /translation="MRCCIRELVLMIAPLLAAAAAAQETPYTQQENVVYGETHGVGLL
                     MDVFTPSGRSNGLAIVDVISGAWHSDRGKIRDHLRAQTFDILCRKGYTVFAIRPGSIT
                     KFSAPEMLANLNSGIRWVKDHAKDYNIDADRLGMMGASAGGHLACLAAVTAEAEKAEA
                     GSQKSTRVKAVAVFFPPTDFLDYGGKPIDVRSDDRLGQMVRRLAVGDGPIPQTADEAT
                     QKVTQISPARLVTPQAPPFLLIHGDADPLVPLQQSQAMLAALKKAGVPAELIVKAGGS
                     HPWPTLHEEVRVIADWFDTQLAARN"
     gene            3719..4636
                     /locus_tag="VFB80_02110"
     CDS             3719..4636
                     /locus_tag="VFB80_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32575.1"
                     /translation="MSRNLNPRYIRVHGVLLTGVMALLALGLTLPAQAQPPVGPTDVA
                     AADKIGVTLYQANGFSGKIPLRLVFEGNPQTDMGHYELKFASDNMNTKMDWKAKFKMP
                     AGMIFKSFELGTANGPIQVTPAGADSWDGQTLIDTVSLSPWNLNRVLQQCIDHLKQPD
                     GSFKDSATFDLQADLTELVRGKGICAAPNAPPGNASSYSGQATPKTRVTAYIVNPLKK
                     ATVDGRPDRLSLPRPARLPPSDSSIGGAGRQPASGSLVLPTGPRLPQPASAGRLIAEP
                     KRTEAKETFKRTKPHVNVGTVPGRLSPQP"
     gene            complement(4721..5941)
                     /locus_tag="VFB80_02115"
     CDS             complement(4721..5941)
                     /locus_tag="VFB80_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32576.1"
                     /translation="MPLLRRSIAQRLALAAACLLSVLCCGQPARAQTPFFGLNWLQPA
                     LVSDWLARFWADDEEQEGTRLATLPDMFGDSFGRGGVLTAASSVPMVTTTSDVLLAGG
                     SRGLKVAEHNKAIPMNRVYLVYNHFHNAAEFSIAAPPPALATTNLPIDRYTLGAETRF
                     GCGLWSLELQMPFTGDLDVVDPGGLAEFHSGQVGNLSAILKRLVYYDGLTSVVLGLGV
                     ETPTGSDFKSRTAVVSYLISNDAVHLHPYLGVLNSPSDSLFFHGFLQLDVATHGNLVT
                     FEDTTGPGNVAGRFNDQTLLYVDVGGGYWLWRDPCGPLVTGLAALLEFHYTATVNDTD
                     SLLATPVGVGGGGHVMSLTNPGNRVDVVNVTAAVHAELDQYTSLRVGGVFPLRGADNR
                     FFDAEVAAQLIRRF"
     gene            complement(6065..8857)
                     /locus_tag="VFB80_02120"
     CDS             complement(6065..8857)
                     /locus_tag="VFB80_02120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32577.1"
                     /translation="MTSKMNAGPAPELAIDESLRRRFEAAWRAGEPLPIEDCLPPRDS
                     AAYLPTLEELVCIELEFAWKSWSAGRASQTWMRDSAPTARPPLLEQYLQRFESLNQPQ
                     VVARLAQQEFLVRTQFGDRPEWEEYRRRFGELAALPDAETIQARRDRHETSRQPLGDG
                     NGDTVAALPARRFGNYELLDELGRGGMGVVYRARQLSADRIVALKVIRSDQLIGLSAR
                     QRAAAVERFHHESLATARLAHEHIVTVYEVGEVNGEPFFSMRYVEGQSLAELLRARPI
                     EGRRAAAYLESVARAVAAAHEAGILHRDLKPQNILVEARSDRALVADFGLAKLLEQPS
                     ELTRAGEVMGTPAYMSPEQARDSSQLTVRSDVYSLGATLYHMLVGRAPFQAATAVETL
                     RQVQHDAPAAPSRLNRAVDRDLETICLTCLQKEPQRRYASAAALADELARYRGGFPIF
                     ARPAGPVERSLRWCRRNPVLAGAIALAATFLGAALVATSVGYATTSAALAGKTRALQE
                     SERSYQQSREVVNQFFTLVSEDVLLNQPGAQPLRSELLQLALKHYQRFLAERGTDPAL
                     LEEIARTHYRVALIQQAIQPEAAAGLPSLEEALRIQRELLAASPANMQLQEALGDTLN
                     ALGQAHQSRSESKTALTLFEESRQFRQRLADRPASGLEPRRKLANTVMNVGLIQKQLS
                     QFDQAARSLDDAQRGRQAALQGRRIEDANSQELLVWRDLARGHYNQANLLAAMKGPAA
                     AEEELPPAIAILEQIRRMAPSDLRNRERLAGCYHLSARLRYERGDLAESSRRYEQAIA
                     VLDDLHRDSPQMVEFQERLALVQMDYGRLQRRRGNLEAALAAYTRACELLQPLVPPPD
                     GTEHPERRRNYAVALRERARAQIDLRQPADAEVLLQLSEQYLTRLVEQAADAPSRKRF
                     ESDLDETRRLRESASN"
     gene            complement(8854..9456)
                     /locus_tag="VFB80_02125"
     CDS             complement(8854..9456)
                     /locus_tag="VFB80_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32578.1"
                     /translation="MPERESMDAWVCALIDGDEAAVAEFWREYGDRLNRLADRHLSPK
                     LQRRVGSEDVIQSVCRTFFRRAREGQFDLPDAEALWRLLCVITLTKCRMLARFHGRER
                     RGLAREQPLDGPAGADGAAPHEPAAADPSPEEAAELADELARLLSGLDAEEQQLVELK
                     LQEQSNEEIAERMNCSQRTVRRVLQRVQSRLRQILNESSS"
     gene            complement(9459..>10230)
                     /locus_tag="VFB80_02130"
     CDS             complement(9459..>10230)
                     /locus_tag="VFB80_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN32579.1"
                     /translation="LWHHHFGRGLVPTPNDFGKQGQPSSHPELLDYLALRFIESGWSL
                     KAMHRMIVTSRTYQLAGQGSGVRGQESGDRAQAELFGRFRRRRLDAESLRDTLLVLGG
                     NLDESVGGEHPFPPQTKWDFTQHKPFKAVYDTNRRSVYLMTQRIQRHPLMAIFDGPDT
                     GASTGSRITSTTTLQALYFLNDPFVHEQAARLAARLQQERSGDAERIERAYQLPFARP
                     PTTDEAQLGLSYLAKAGDAAWESYIRSLLRTNEFVYVE"
BASE COUNT         1739 a         3437 c         3360 g         1694 t
ORIGIN      
        1 tccgatcgca gccggctgca gtcagccgcc gccgcccctg ccgcccgcga accgagagag
       61 accgccgccg catgaaattg gattcgctgc ccgtcacctg tgaagccacg atccccgagt
      121 cgtatctcga tgtgatgggg cacatgaacg tgatgtggta cacgcacctg ttcggcaagg
      181 ccgctgtcgg gctgttccgc ctcgccggaa tggatcggca gtacttcctg gaccaccggg
      241 ccggcacctt tgccctggcc caggtttttc gctatcgcaa ggaagtgcgg gtaggcgagc
      301 acgtcgcgat ccgcagccgg ctgatcgccc gctcggagcg gcagtttcac gtgatgcact
      361 tcatgaccaa gggggatgag cagatcttgg ccgcgaccgg cgagtttctg gcggcgcaca
      421 tcgacatgca aacccgccgc acggccccct ttgccccaca catcgcccag aacctggatc
      481 gcctcctggc cgagcactcg gccctcgatt gggccgcccc ggtcagcggc gcgatcgagg
      541 ttcgctagaa gccgcttatg ttccccgagc cttcaccgcg cgccacagag cttcgccaac
      601 gactcgtggc gttcatggac gagcacatct atccgagcga agcgcagctg ggcgcgcacg
      661 atcaatcggc cgaccgctgg cagccgccac agttgctgga gcagctcaag gccgaggcga
      721 aagccgcggg gctgtggaac ctgtttctac cggaccgccg gcacggcgcg gggctgacca
      781 atctagagta cgcgccgctc tgcgagatca tgggccgctc gccgatcgcg cccgaagtct
      841 tcaactgctc ggcgcccgac accggcaaca tggaggtcct cgcccgctat ggcacgccgg
      901 agcagcagga gcgctggctc gcgccgcttc tggccggcga aatccgctcc tgctttgcca
      961 tgtccgaacc cgacgtcgcc tcgtccgacg cgacgaacat ccgctcgcgg atcgagcgcc
     1021 gcggcgacga atatgtcatc aacggccgca agtggtggac ctccggcgcg ggcgacccgc
     1081 gctgccagct gtgcatcttc atgggccaga ccgatccggc ggcggcggtg cacgcgcagc
     1141 aatcgatgat cctggtgccg atggacgcac caggcgtcaa agtccagcgg atgctgacgg
     1201 ttttcggcta cgaccacgct ccgcacggcc atgcggagat cgtcttcgac aatgtgcgcg
     1261 tgccggccgc gaatatcctg ctgggcgagg gacgcggctt cgagattgcg caggggcggc
     1321 tggggccggg gcgaattcac cactgcatgc gattgatcgg cgtggccgag cgctcgctgg
     1381 aggcgatgtg ccggcgcgtt ttgacgcgag ttgcgttcgg caagccgctc gccgagcagg
     1441 gcacgattcg cgccgagatc gccgactcgc ggatcgagat cgagcaggcc cgtttgctga
     1501 cgctcaaggc ggcacatctg atggacacgg ttggcaacaa ggaagcccga gccgaaatcg
     1561 ccatgatcaa ggtcgcctgc ccgaacatgg ctctccgcgt gatcgaccgc gccatccagg
     1621 cccacggcgg agcgggcgtc agcgccgatt tcggcctcgc cgccgcctgg gccatgaccc
     1681 gcgcgttgcg cctggccgat gggccggatg aagtccatcg cgaggcgatt gcaaaactgg
     1741 agctgaagag gcacaaatcc caaatctgaa atctgaaatc tgaaatggaa tcgccatgag
     1801 tgtatcgatt tccctcaccg gcaaagtcgc cctcgtcacc ggcggcggcc ggggcatcgg
     1861 caaggcgatc acccagcacc tggccgccgc cggcgcgaat gtcgtcatcg ccagccgcaa
     1921 gctgtcaaac ctccaggcgg cagccgaaga actttcgtcg ctgccgggca aggtcgtccc
     1981 gatcgagtgc cacgtcggcc gcctcgacca gctgcagagt ctggtccgcg aaacggaggc
     2041 ccgcctgggg ccggtcgaga tcctcgtcaa taacagcgcc acgaacatcg gccagggccc
     2101 ctcgctcgac gtcaccgacg aaatgctcga caagatgatc gagatcaacg tcaaggcggc
     2161 gctgcggctg gtgcggctga ccgtgcccaa gatgctcgag cgccagagcg gggcaatcat
     2221 caacatcgcg tcgatcgccg gtctgcaccc gcagccgcag agcctgttgt acagctttac
     2281 gaaggcgggt ctgatcatga tgacccgcag ttgggcccgc gaattcgggc cgcagggcgt
     2341 gcgggtgaat gcgatcgcgc cgggcctgat ccagaccgat ttcagtgagt actactggaa
     2401 ggacgacgag cgccgccggc agatcgaatc gcggcagcca atcgcgcgaa tcggccagcc
     2461 ggaggagatt ggccacctgg cgctgtacct ggcgtcgagc gaagcgtcgt acgtcaccgg
     2521 gcaggtgttt gtcgtcgatg gcggcgcgac cgcgtagcaa gcggcgtcaa ttcctggccg
     2581 ccagctgcgt gtcgaaccaa tcggcgatga cgcggacttc ttcgtgcagc gtcggccagg
     2641 gatggctgcc gccggccttc acgatgagct cggccggcac gcccgctttc ttcagcgcgg
     2701 caagcatcgc ctgcgactgc tggagcggga cgagcggatc ggcgtcgccg tgaatgagca
     2761 ggaagggcgg cgcttgcggc gtgaccaggc gggcgggtga aatctgcgtg actttttgcg
     2821 tcgcttcgtc ggcagtctgg ggaattggcc catcgcccac cgcgagtcgc cggaccatct
     2881 gccccagccg gtcgtcgctc cgcacgtcga tgggcttgcc gccatagtcc aggaaatcgg
     2941 tgggaggaaa gaacacggca acggccttca cgcgcgtgct cttctggctg ccggcttccg
     3001 ctttctcggc ttcggccgtc accgcggcga ggcacgccag gtggccgccg gccgaagccc
     3061 ccatcatgcc caggcggtcg gcatcgatgt tgtagtcctt cgcatggtcc ttcacccagc
     3121 ggatgccgct attcaggttg gccagcattt cgggtgcgct gaacttcgtg atcgagcccg
     3181 ggcgaatcgc gaagacggtg tagcccttcc ggcacaggat gtcgaacgtc tgcgcccgca
     3241 ggtgatcgcg aatcttgccg cggtctgaat gccacgcccc gctgatcacg tccacgatgg
     3301 ccagcccgtt gctccggccg ctgggcgtga agacgtccat cagcagtccg acgccgtgcg
     3361 tttcgccata cacgacgttc tcctgctgcg tgtagggcgt ctcctgcgcc gcggcagccg
     3421 ccgccagcag cggcgcgatc atcaacacca actcgcgaat acagcacctc acggctgatc
     3481 tcctttgtga gtcgggaaat gtgcgggcat tgtacccaaa ctggctgccc accagggttt
     3541 agccaaattc cttgcagaat tttgtccgcc tccgcggcgg gacggcaata caggggcaga
     3601 gcagcaggcg accagccgaa cttgatggcg ggccgccagc ttcacaagac cagcgcctcg
     3661 gttgcggcgg cccgcgctga tcgattccat tctccccgcc gaatgaaagt cttcagatat
     3721 gtctcgcaac ctcaaccctc gttacattcg cgtgcacgga gtcctgctga ccggagtgat
     3781 ggccctactg gccctcggac tgacattgcc agcccaggcg cagccgcctg tgggaccgac
     3841 cgacgtggcc gccgcggaca agatcggcgt gacgctctat caggccaacg gcttcagcgg
     3901 aaagatcccg ctgcggctgg tctttgaggg aaacccgcag accgatatgg ggcactacga
     3961 gctgaaattc gcctccgaca acatgaacac taagatggat tggaaggcca agttcaagat
     4021 gcccgccgga atgatcttca agagctttga gctggggacc gccaacgggc cgatccaggt
     4081 cactccggcc ggagccgact cgtgggacgg gcagacgctg atcgacacgg tctcgctctc
     4141 gccctggaac ctgaaccgcg tgcttcagca gtgcatcgat catctgaagc agcccgacgg
     4201 cagcttcaag gactccgcga cgttcgatct gcaggccgac ctgaccgagt tggtccgcgg
     4261 caagggcatt tgcgccgctc ccaacgcccc gccgggcaac gcctcgagct actccggcca
     4321 ggcgacgccc aagacccgcg tcaccgccta catcgtcaac ccgctgaaga aggcgactgt
     4381 tgacggccgc cccgaccggc tctcgttgcc gcggcccgcc cgtctgcctc cgagcgattc
     4441 aagcatcggc ggcgctggcc gtcagccggc ctcgggttcg ctggtgctgc cgaccggccc
     4501 ccgcctgccg cagcccgcgt cggctggccg cctgatcgcc gagccgaagc ggacggaagc
     4561 gaaggagacg ttcaagagga ccaagccgca cgtcaacgta gggacggtgc ccgggaggct
     4621 gagcccgcag ccgtagggcc gtgagggagg agaggcgagg cgattcgcct gggaatgccc
     4681 ggacttgttc cgggcattct ctgtttcttg ccttccgcga tcagaaccgg cggatgagct
     4741 gggccgcgac ttcggcgtcg aagaagcgat tgtcggcgcc ccgcagcggg aagacgccgc
     4801 cgacgcgaag gctcgtgtac tggtccagct ctgcatgaac ggccgcggtc acgttgacga
     4861 cgtccacgcg attgcccgga ttggtgagcg acatcacgtg cccgccgccg ccgactccga
     4921 ccggcgtggc gagcagcgag tcggtgtcgt tcacggtggc cgtgtagtgg aattccagca
     4981 gagctgccag cccggtgacg agcggcccgc aggggtctcg ccacagccag tagccgccgc
     5041 ccacatcgac atagagcagc gtctggtcgt tgaacctgcc cgcgacgttg cccggtccgg
     5101 tagtgtcctc gaacgtgacg aggttgccgt gcgtcgccac gtcgagctgc aggaagccgt
     5161 ggaagaaaag gctatcgctg ggtgagttga gcacgcccag gtacggatgc agatgaaccg
     5221 cgtcgttgga gatcaagtag ctgacgacgg ccgttcgcga cttgaagtcc gatccggtcg
     5281 gcgtctccac gcccaggccc agcacgacgg aagtcaggcc atcgtaatag acgagccgct
     5341 tgagaatcgc cgacaggttg ccgacctggc cgctgtggaa ctccgcgagt ccgccgggat
     5401 cgaccacgtc gaggtcgccc gtgaagggca tctgcaactc gagcgaccac agcccgcagc
     5461 cgaaccgggt ttccgcgccg agcgtgtagc ggtcgatcgg caagtttgtc gtggcgaggg
     5521 ccggcggcgg cgctgcgatg ctgaactcgg cggcgttgtg aaaatggtta tagaccaggt
     5581 agacgcgatt catgggaatc gccttgttgt gctctgcgac cttcaggccc ctcgagccgc
     5641 cggccagcag cacgtcgctg gtcgtggtca ccatcggcac tgagctggcg gccgtcagga
     5701 cgccgccgcg gccaaagctg tcgccgaaca tgtcgggcag ggtcgccagc cgcgtgccct
     5761 cttgctcctc gtcgtcggcc cagaatcgcg ccagccagtc cgacaccaat gccggctgca
     5821 gccagttgag gccgaaaaac ggcgtctggg cgcgcgccgg ctggccgcag cacaggaccg
     5881 acagcaggca tgccgccgcc agcgcgaggc gctgagcgat gctgcggcgc aaaagcggca
     5941 tgatgactcg accggaacga gtgaggctgt gcgggcgcac ttctcaactg gcatcggcac
     6001 gggttcgcgc cgcgaagcac tacgaacgct acaaccctca cgccttgccc agcgcccgca
     6061 gctttcagtt gctagcggac tctcgcagtc gcctggtttc gtccaggtcg ctctcgaaac
     6121 gcttccgcga gggtgcgtcc gccgcttgtt cgaccagccg cgtcaaatac tgctcggaca
     6181 attgcagcag aacctcggcg tcggccggct gccgaaggtc gatctgcgct cgggcccgct
     6241 cgcggagcgc gacggcgtag ttgcggcggc gctcggggtg ctcggtcccg tcgggcggcg
     6301 gaacgagcgg ctgcagcagc tcgcaggcgc gggtgtaagc cgccagcgcg gcctccaagt
     6361 ttccccgccg tcgctggagc ctgccgtagt ccatctgaac gagggccagc cgctcctgaa
     6421 actcgaccat ctgcgggcta tcgcggtgca ggtcgtcgag caccgcgatc gcctgctcgt
     6481 atcgccgcga cgattctgcc aaatcgcccc gctcgtaacg cagccgggcc gagaggtggt
     6541 aacacccggc caggcgctcg cgattgcgca agtcgctggg cgccatgcgc ctgatctgct
     6601 ccagaatggc aatcgccggc ggcagttcct cctcagcggc ggccgggccc ttcatggcgg
     6661 ccagtaggtt ggcctgattg tagtgacccc tggccaggtc gcgccacacg agcagctcct
     6721 gggagttcgc gtcctcgatc ctgcggcctt gcagcgccgc ctggcggccc ctctgagcat
     6781 cgtcgagcga ccgcgccgcc tggtcgaact gcgacagctg cttttgaatc aggccgacat
     6841 tcataaccgt attggcgagc ttgcggcgag gttccaggcc actggctggc cgatccgcca
     6901 agcgctggcg gaactggcgc gactcttcga ataacgtcaa cgccgtcttg ctctcgctgc
     6961 ggctctgatg cgcttggccc agggcattaa gcgtgtcgcc cagggcctcc tggagctgca
     7021 tattcgcggg cgacgcggcg agtagctccc gctggattcg tagcgcctcc tccagtgacg
     7081 gaagaccggc ggccgcctcg ggttgaatcg cctgctggat caaagctacg cgatagtgcg
     7141 tgcgggcgat ctcttccaag agcgccggat ctgtgccgcg ctcggccaga aaccgctgat
     7201 agtgcttcag cgccaactgg agcagctcac tgcggagcgg ctgagccccc ggctgattga
     7261 gcagcacatc ctcgctcacc agcgtgaaga actgattcac tacctcgcgc gattgctgat
     7321 aactccgttc cgactcttgc agggcccgcg tcttgccggc gagcgcggcc gacgtcgtgg
     7381 catagcctac gctggtggcg acgagtgccg cgcccaggaa agtcgccgcc agggcaatcg
     7441 ccccggcgag cacgggattg cggcggcacc accgcagcga gcgttcgacc ggccccgcgg
     7501 gtcgggcgaa aatcggaaag ccgccgcgat accgcgccag ttcgtcggcc agggcggcgg
     7561 ccgacgcata tcgtcgctgc ggctctttct gcaggcaggt caggcagatc gtctccagat
     7621 cgcgatcgac ggcccggttc agccgcgacg gcgcggccgg cgcgtcgtgc tgcacctgcc
     7681 gcagggtctc gactgccgtc gcggcctgaa acggcgcgcg gccgacgagc atgtgataca
     7741 gcgtcgcacc gagggaatag acgtcgctgc gcactgtgag ctgcgacgag tcgcgggcct
     7801 gctccggcga catataggcc ggcgtaccca tgacttcgcc ggcccgcgtc agctcgctcg
     7861 gctgctccag cagcttggcc aggccgaaat cagcgaccag ggcccggtcg ctccgcgcct
     7921 cgaccaggat gttctgcggc ttcaggtcgc gatgcaaaat acccgcctcg tgggccgcgg
     7981 cgactgcgcg ggcaacggat tccaggtacg cggctgcccg gcggccttcg atcggacggg
     8041 cccgcagcaa ttccgccagg ctctgtcctt cgacgtagcg catcgaaaag aacggctcgc
     8101 cattgacctc gcccacttca tagaccgtga cgatgtgctc gtgcgcgagg cgggcggtgg
     8161 ccagcgattc gtggtggaac cgctcgaccg ccgcggctcg ctgccgggcg gaaaggccga
     8221 tcaactggtc gctgcgaatc accttcaagg cgacgattcg gtcggcggaa agctgcctgg
     8281 cccgatagac cacgcccatt ccgccgcggc cgagctcgtc gagcagctcg tagttgccga
     8341 agcggcgcgc cggcaaagcg gccacagtgt cgccattccc atcgccgagc ggctggcggc
     8401 tggtttcgtg acggtcgcgg cgggcttgaa tggtctcagc gtcgggcagc gccgccagct
     8461 cgccgaatcg ccgccgatat tcttcccact cgggccgatc gccgaattgc gttcggacaa
     8521 ggaattcctg ctgggccagg cgggcgacga cctgcggctg attcagcgat tcgaagcgct
     8581 gcaagtactg ttcaaggagc ggcgggcggg ccgtgggagc gctgtccctc atccacgtct
     8641 gactcgcgcg ccccgccgac caggacttcc aggcaaactc caactcgatg caaaccagct
     8701 cttccaacgt cggcaggtac gccgcgctat cgcgcggagg aaggcaatcc tcgattggca
     8761 gcggctcgcc ggcccgccag gcagcttcga agcgccgccg cagcgattcg tcgatcgcca
     8821 gttcgggcgc cgggccggca ttcattttcg aggtcacgat gacgattcgt tcaaaatctg
     8881 tcgcaagcgc gactgaaccc gctggagcac gcgccgcacg gtgcgttgcg agcaattcat
     8941 ccgctcggca atttcttcgt tgctctgctc ctgcaacttc agctcgacga gctgctgctc
     9001 ctcggcgtcc aagcccgaca gcagccgcgc cagctcgtcg gccagctccg cggcctcttc
     9061 cggcgatgga tcggccgccg ccggctcgtg cggcgccgcg ccgtccgcgc cggccggccc
     9121 atccagcggc tgttcccgcg ccagaccgcg ccgctcgcgg ccgtgaaagc gggccagcat
     9181 ccggcacttg gtcagcgtga tgacgcagag cagccgccag agcgcctccg catccggcaa
     9241 atcaaattgg ccttcccgtg cgcggcggaa gaaggtccgg cagaccgact ggatcacgtc
     9301 ttccgatccg acccggcgtt gcagcttcgg tgaaaggtgc cgatcagcca ggcggttcag
     9361 ccgatcgccg tactcgcgcc aaaactcggc aactgccgct tcgtcaccgt cgatcagtgc
     9421 gcaaacccag gcgtccatcg attcacgctc gggcatggct actccacgta cacaaactca
     9481 ttcgtccgta gcagcgatcg gatgtagctt tcccaggcgg cgtcgccggc cttggccagg
     9541 tagctcagac cgagctgggc ttcgtccgtc gtcggtgggc gagcgaaggg cagctgatag
     9601 gcgcgctcga tccgctccgc atcgcccgag cgttcctgct gcaagcgggc ggcgaggcgg
     9661 gcggcctgct cgtgaacgaa ggggtcattg aggaagtaaa gggcctggag cgtcgtggtg
     9721 ctggtgatcc ggctgccggt gctggcgccg gtgtcggggc cgtcgaagat cgccatcagc
     9781 ggatgccgct gaatccgctg cgtcatcaaa tagacgctgc ggcggttggt gtcatagacg
     9841 gccttgaatg gcttgtgctg ggtgaaatcc cacttcgtct gtggcggaaa cggatgttcg
     9901 ccgcccaccg actcgtcgag attgccgccc agcaccagca gcgtgtcgcg gagcgactcg
     9961 gcgtccagcc gccggcggcg gaaccggcca aagagctccg cctgtgctct gtcgcctgac
    10021 tcctgacccc tgactcctga cccctggcct gcgagctgat acgtccgcga cgtgacgatc
    10081 atccggtgca tggccttgag cgaccagccg ctctcgatga accgcaaggc cagatagtcg
    10141 agcagctcgg ggtgcgagga gggctggccc tgcttgccga aatcgttggg cgtgggcacc
    10201 aggccgcggc cgaagtgatg atgccagagg
//
LOCUS       DASRJB010000077         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1717024, whole genome shotgun sequence.
ACCESSION   DASRJB010000077 DASRJB010000000
VERSION     DASRJB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717024"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFB80_02135"
     CDS             <1..1336
                     /locus_tag="VFB80_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HZN32580.1"
                     /translation="LTCRGGVSSHAALVARQMGKVCVAGAGDIQIDYHAGKLTCGGVT
                     LREGDAISINGSTGEVFHGAIDTADSELKQVLIGKTLKAGDSKVFQYYNSLMKLADKY
                     RTLGIRTNADQPDQVVNAVAFGAEGIGLCRTEHMFFEGDRIDSMREMILADNEADRKA
                     ALAKLLPHQQKDFEGIFRALDGLPACIRFLDPPLHEFLPHEHGQQMALAGKLHLSPEK
                     ISQRVHELHEFNPMLGFRGCRLGIKFPEISEMQARAVFQAAAAVMKEGIKVKPEIMIP
                     LVGFKKELDLQVEIVHRVAKEVMKETGKKIPYTVGTMIEVPRGALTADEIAETAEFFS
                     FGTNDLTQTCLGMSRDDSGSFLPAYSGAEIIKTNPFASIDTTGVGQLMKIGVDKGRSR
                     RGELKIGICGEHGGDPASVVFCHKIGLNYVSCSPFRVPIARLAAAQAALKGN"
     gene            complement(1439..2281)
                     /locus_tag="VFB80_02140"
     CDS             complement(1439..2281)
                     /locus_tag="VFB80_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32581.1"
                     /translation="MAIVALSTNLASAAEPTRLTTDGLLKQRPAWSPDGTQLAFTRHQ
                     GATIFLFIRSADGSERRLTKSKDPEFDAHWSPDGKRLVFAFDKTVPNQGDMEVYSIAA
                     DGSDLQPVLTSPGKLTHEEWPSWSPDGTRIALSSTRDGNQEIYTVQPDGKDLKRLTND
                     PAIDAHPCWSPDGQRVAFATNRWGDLEIAVVNADGSNLVRLTDNRGFDDYPAWSPDGK
                     TIAFTSYRDGNPEIYAIDPDGKNPRNLTQHPALDNFAAWSPAGELTFVSNRGGGSDIY
                     KQQP"
     gene            complement(2413..>3697)
                     /locus_tag="VFB80_02145"
     CDS             complement(2413..>3697)
                     /locus_tag="VFB80_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32582.1"
                     /translation="TLADLLALEARAAPNLAQPRAKSVILLWLWGGPSHLDTFDMKPK
                     APLEYRGPYAPVPTSVPGIQICELLPQLASRADRYAIVRSLHSTSNDHGIAGTIGLTG
                     SEAGGASLGGQVQPGQVQPNHGAIVSRVLGFDPATPRFVTLGGHLHQGKKPIRGEGGG
                     TVGPLHDPFRLDYDPEAGVKIPQLDLLAGLSADGLVSRSQLRAALDDLTRQLDGSPQL
                     ERLDQFRRQAFSLLTSPAAREVYDLSREPDELRTKYGRFRFGQCCLMARRLIEAGVRF
                     VQVNWSSHVEPVEDTGDGGWDMHDRNFQQMQDRHAWMLDQSLAALLDDLQDRGRLSET
                     VVVAVGEFGRTPKINEKAGRDHWQQCYSGLIAGGGIQVGQVIGESDAKAEFPANRPVT
                     PADLFRTALHQIGIGTTQLTAAGLTPLGEVIEELV"
BASE COUNT          642 a         1246 c         1199 g          610 t
ORIGIN      
        1 cctcacctgc cgcggcggcg tttccagcca cgcggccctg gtcgcccggc agatgggcaa
       61 ggtctgcgtc gccggcgcgg gcgacattca gatcgactat cacgccggca agctgacctg
      121 cggcggcgtc acgctcaggg aaggggacgc gatcagcatc aacggctcga ccggtgaggt
      181 ctttcacggc gcgatcgaca cggccgacag cgagctgaag caggtgctga tcggcaagac
      241 gctcaaggcc ggcgacagca aggtctttca gtactacaac tccctgatga agctcgccga
      301 caagtaccgc acgcttggca tccgcaccaa cgccgatcag ccggaccagg tcgtcaacgc
      361 ggtcgccttc ggcgcggaag gcatcgggct ctgccgcacc gagcacatgt tcttcgaggg
      421 cgaccgaatc gactcgatgc gggagatgat cctggccgac aacgaggccg accgcaaagc
      481 ggccctagcg aaactgctcc cgcaccagca gaaggatttt gaggggatct ttcgcgccct
      541 ggacggcctg ccggcctgca tccgcttcct cgatccgccg ctgcacgagt tcctgccgca
      601 cgaacacggg cagcagatgg ccctggccgg caagctgcac ctctcgcccg aaaagatctc
      661 ccagcgcgtc cacgagttgc acgagttcaa cccgatgctt ggcttccgcg gctgccggtt
      721 gggcatcaag ttccccgaaa tctcggagat gcaggcccgc gctgtcttcc aggccgcggc
      781 ggcggttatg aaggagggga tcaaggtcaa gcccgagatc atgatcccgc tggtcggctt
      841 caagaaggag ctcgacctgc aagtcgagat cgtgcatcgc gtggccaagg aagtgatgaa
      901 agagaccggc aagaagattc cgtacacggt cggcacgatg atcgaggtgc cccgcggagc
      961 attgacggcc gacgagatcg ccgagaccgc cgagttcttc agcttcggca ccaacgacct
     1021 gacgcaaacc tgcctgggca tgagccgcga cgacagcggc tcgttcctgc cggcctacag
     1081 cggcgcggag atcatcaaga ccaacccgtt cgcctcgatc gacacgaccg gcgtcgggca
     1141 gctcatgaag atcggcgtcg ataagggccg cagccggcgg ggcgagctga agatcggcat
     1201 ctgcggcgag cacggcggcg atcccgcctc ggtcgtgttc tgccacaaga tcggcctgaa
     1261 ctacgtcagc tgctcccctt tccgcgtccc catcgcccgc ctcgccgccg ctcaggcggc
     1321 gctaaaggga aattaaccgc ttagccccgg cccccaggcc ggttgccggg gtcgaagacc
     1381 cacgcagcac cggcgcagcc caaggtgggc ctgcgctcgg ggactcgctt gacccaccct
     1441 acggctgctg tttgtagatg tcgctgccgc cgccgcgatt ggagacaaac gtcagctcgc
     1501 ccgcgggcga ccaggcggcg aagttgtcga gggcgggatg ctgcgtgaga ttccgcggat
     1561 tcttcccgtc gggatcaatc gcatagatct ccgggttgcc gtcgcgatag ctcgtaaagg
     1621 cgatcgtctt gccgtccggc gaccaggcgg gatagtcgtc gaagccgcgg ttgtcagtga
     1681 ggcgtaccag gttgcttcca tccgcattga cgacggcaat ctccaggtcg ccccagcggt
     1741 tcgtggcgaa ggccacgcgc tgcccgtcag gcgaccagca ggggtgggcg tcgatcgccg
     1801 gatcgttcgt cagccgcttg aggtccttgc cgtccggctg cacggtgtaa atttcctggt
     1861 tcccgtcgcg ggtcgagctg agcgcgatcc gcgtgccgtc gggcgaccag ctcggccact
     1921 cctcgtgcgt gagcttgccg gggctcgtca gcacgggctg caaatcgctg ccatccgcgg
     1981 cgatcgagta gacttccatg tcgccctggt tcggcacggt cttatcgaag gcaaacacca
     2041 gccgcttgcc atccggcgac cagtgggcgt cgaactccgg atctttggat ttcgtcagcc
     2101 gccgctcgct gccatccgcg gaacggatga acagaaagat tgtcgcgccc tgatggcgag
     2161 tgaaggcgag ctgcgttcca tccggcgacc aggcgggccg ctgtttgagg agaccgtcgg
     2221 tggtgaggcg ggtgggctcg gcggcggacg cgagattcgt gcttagagcg acaatcgcca
     2281 acaggcaatt gcaaaatgcg aattgcaaat tgcaaattgc aaaatgagcc ggtcgcttcg
     2341 gcaatttgca ctttgcactt tgcatttttc aatttgcaat cgctgcagaa agagcgccgc
     2401 acattcgacc ggctaaacca gctcctcaat cacctcgccc agcggcgtca ggccggcggc
     2461 ggtcagctgc gtcgtgccga tgccgatctg gtgcagcgcg gtgcggaaca ggtcggcggg
     2521 cgtcaccgga cggttcgcgg ggaactcggc cttggcgtcg ctttcgccga tcacctggcc
     2581 gacctggatg ccgcccccgg cgatcagccc cgagtagcac tgctgccagt ggtcgcggcc
     2641 ggctttttcg ttgatcttcg gcgtgcgtcc gaattccccc accgcgacga cgacagtctc
     2701 cgacaagcgg ccgcggtcct gcaaatcgtc caagagcgcc gccagcgact ggtcgagcat
     2761 ccaggcgtgg cggtcctgca tctgctgaaa gttgcggtcg tgcatgtccc agccgccgtc
     2821 gccggtgtcc tcgaccggtt cgacgtgcga gctccaattc acctgcacga accgcacgcc
     2881 cgcctcgatc agccggcggg ccatcaggca gcactgcccg aagcggaagc ggccgtactt
     2941 cgtccgcagt tcgtccggct cgcgcgacag gtcgtacacc tcgcgggccg ccggcgaggt
     3001 gagcagcgaa aaggcctgcc ggcggaactg gtccagccgc tcgagctgcg gcgagccgtc
     3061 gagctgccgc gtcaggtcgt ccagagccgc acgcagctgg ctccgcgaca ccagaccgtc
     3121 ggccgaaagc ccggccagca gatcgagctg aggaatcttc acgcccgctt cggggtcgta
     3181 gtccagccgg aagggatcgt gcaaggggcc gacggtgccg ccaccttcgc cgcggatcgg
     3241 cttcttgccc tggtgcaggt gtccgcccag cgtgacgaac cgcggcgtcg ccggatcgaa
     3301 acccagcact cgcgacacga tcgccccgtg gttcggctgc acctgccccg gctgcacctg
     3361 cccgcccagg ctcgcccccc cggcctccga accggtgagc ccgatcgtgc cggcgatgcc
     3421 gtggtcgttc gaggtcgagt gcaacgagcg gacgatcgca tagcggtcgg cccggctggc
     3481 cagctgcggc agcaattcgc agatttgaat ccccggcacg ctcgtcggga ccggagcata
     3541 cggccctcga tattccagcg gcgccttggg cttcatgtcg aacgtgtcga ggtggctcgg
     3601 cccgccccac agccagagca aaatcaccga cttcgcccgc ggctgcgcca gattcggcgc
     3661 cgcgcgggcc tccagcgcga gcaaatcggc cagggtc
//
LOCUS       DASRJB010000078        10242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3409813, whole genome shotgun sequence.
ACCESSION   DASRJB010000078 DASRJB010000000
VERSION     DASRJB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409813"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFB80_02150"
     CDS             <1..422
                     /locus_tag="VFB80_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32583.1"
                     /translation="IADRAGNRRVLILVTLLIAGGPLAALVCIYAPEFGKRAFPLVFT
                     LVGLTPVAQKTFNNYTLEITTPENHPRYLSTLSLSMAAPICASPLVGYAIELVGFEVV
                     YLGVVGLLLVGWLMSFGLTEPRAGGRPVVLAEDSITE"
     gene            560..763
                     /locus_tag="VFB80_02155"
     CDS             560..763
                     /locus_tag="VFB80_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32584.1"
                     /translation="MDGSIDERRRAELLSLWKSNPHRVLSLYRQVVDLDELAPLPAGT
                     TVTHLIDVILEKAACDDSLSLQP"
     gene            839..1138
                     /locus_tag="VFB80_02160"
     CDS             839..1138
                     /locus_tag="VFB80_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32585.1"
                     /translation="MLPDSTPPCPACGEPNAATPAWQLVDEEGRELLRCGCGHVFAVE
                     PAQNLSVKEVIDRARTAQLAFRLEYQLHEDLHREFEQLKRRFRHGRGRGITPPAD"
     gene            complement(1145..1414)
                     /locus_tag="VFB80_02165"
     CDS             complement(1145..1414)
                     /locus_tag="VFB80_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN32586.1"
                     /translation="MVLAPGAEEGGADNRHRGADQWLFVVSGAGRATINRRRHRLREG
                     SLLLIERGDTHLIQNTSRKRQLVTLNIYVPPAYTPSGDERAAGKP"
     gene            1613..1819
                     /locus_tag="VFB80_02170"
     CDS             1613..1819
                     /locus_tag="VFB80_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32587.1"
                     /translation="MAVRGCLVRCADSDTPLTSLAEFAEELRRLGWDKKSIRDVEFEV
                     IQVLGRRDGRDRRNQHRQKNSDSA"
     gene            complement(1852..4839)
                     /locus_tag="VFB80_02175"
     CDS             complement(1852..4839)
                     /locus_tag="VFB80_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZN32588.1"
                     /translation="MAGGKKSSKKGLVQGRSPARLRTAPSAADDDLEFTPTVVGVGAS
                     AGGLEAFSQLLRALPADTGLALVFVQHMAPHHESALASLLASSTSLPVIQPTEDSRLE
                     PDHVYVIPPNRGLEVQNGRLIIQPRPTDRTQYLPIDHFFQSLAKAYGSDAVGVILSGT
                     GSDGVEGMHAIKAAGGTTLAQDPATAKQPGMPQAVISTGKIEYALSPAELAQELARIA
                     RNRAESPAESRRAAEAALLDPEHLQQIFEILRRVSGVDFGQYKLPTVQRRIQRRMALH
                     KVGALEQYVSVLRENQAEANNLYQDILIHVTRFFRDPDSFRQLATETIPAILAARQGE
                     APLRIWVPACSTGEEAYSVAMTIMEHVEERNERVPIQIFATDISETAIEQARAGVYPP
                     NVVEELSPERLRRFFTKTDGQYQINKPIRDLCVFARQDLTRDPPFSKLDLIVCRNVLI
                     YLGQPLQHRLIGVFHYALKPTGYLMLGSAETIGPHGELFAVCDKKHHIYRKKLGETPQ
                     NVQFTVPYTRPTFGRAARAGEEARGGGNVQSEANRLILDRYSPPGVIVNDDLRIMQFR
                     GQTGRFLEPAPGDASLNLLKMCREGLLHGLRTALQTAKKKDAPVRREGLRVKTNGDFV
                     NVNVDVVPLTPSAEGRHYLILFQGVAKSTAPPLKIASVGKKGTGKQAEEVERLQKELV
                     ASREYLQSVIQDLEAANEELQSANEEILSSNEELQSTNQELDTAKEELQSTNEELNTV
                     NEELHFRNDELARINSDLINLINSVQIAIVIVAGDLRIRRFTPMAERILNLIPSDIGR
                     PISQIKPNINGPELEKLILEVIDTISVQQRDVLDSQGRTLSLTIRPYKSVDNRIDGAV
                     LTLFDVDEIRRHELQAQEAREYAEAVFNTVREPLLVLDGKLRVQTVNPAFCRVFGVTL
                     DDVRGRLLYELGAGAWNNPRLRNLLEDVLPSDSHFEDFEIRQEFAGVGMKQIRLNARR
                     ISGSDHRPALILLAMEGDKQPAS"
     gene            5052..5306
                     /locus_tag="VFB80_02180"
     CDS             5052..5306
                     /locus_tag="VFB80_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32589.1"
                     /translation="MSREMEDFWMHIHRLSSALDDAGITRDERVRHLLQQLEQLPPLV
                     RRELLLELTQVAADILDLQPLAVAAMNKLEEPAVQSRQMS"
     gene            complement(5355..5540)
                     /locus_tag="VFB80_02185"
     CDS             complement(5355..5540)
                     /locus_tag="VFB80_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32590.1"
                     /translation="MKSTKQKPTRARRKPQPRDKEIVFSCSPYEAALSDMPLNAIQNV
                     QVNHIVRAVACRGGPCR"
     gene            complement(5556..6224)
                     /locus_tag="VFB80_02190"
     CDS             complement(5556..6224)
                     /locus_tag="VFB80_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HZN32591.1"
                     /translation="MLFRNRVEAGRRLAAELTNYADRPDVLVLALPRGGVPVGYEVAR
                     ALHAPLDVFLVRKLGVPGRPELAMGAIASGGTRVLNEELLGMLSVPPRRIAEVAQREQ
                     RELDRRERLYRGSRLPPEIREKTVILVDDGLATGSTMRAAVLALRQLQPARIVVAVPV
                     GAKQTCAEFESEADETICATTPEPFHAVGAWYDNFDPTSDDEVRRLLARASEELRAAV
                     PAAT"
     gene            complement(6342..6776)
                     /locus_tag="VFB80_02195"
     CDS             complement(6342..6776)
                     /locus_tag="VFB80_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN32592.1"
                     /translation="MLVRELMTRGVQCVSPGESIAGAAEKMKRLNVGSLPICGEDDKL
                     AGMITDRDITVRATAECCEPRSTTVGEVMTPGVVYCFDDQPIDEVAGLMEKHQIRRLP
                     VINREKRLVGIVSLGDLAVKHRDDRLSGEALERISEPAVPLR"
     gene            complement(7983..8168)
                     /locus_tag="VFB80_02200"
     CDS             complement(7983..8168)
                     /locus_tag="VFB80_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32593.1"
                     /translation="MLRLAIVDSDGKHMDAKEIASAVDLVSIAEQARTIIRDCEQLAR
                     DKRPRQTAGTGRVPYST"
     gene            complement(8580..8852)
                     /locus_tag="VFB80_02205"
     CDS             complement(8580..8852)
                     /locus_tag="VFB80_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HZN32594.1"
                     /translation="MKLCLLAQAAGDGVGAAGGALALGGGMMLVMLILGLLCLVLWIW
                     ALIDAIQNPGLNQTERLIWVLVILLTNTLGAILYLLIGRSRRAMTT"
     gene            9025..9498
                     /locus_tag="VFB80_02210"
     CDS             9025..9498
                     /locus_tag="VFB80_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN32595.1"
                     /translation="MFSISGLVESPRSLTFADLAAIGAEHQVADVSRLVPGRKGDAVR
                     LAGILELVRPKEAAKWLGLHAAADNFHASIPLDAVADKALVIYRLDGQPLPAKAGGPV
                     RFFIPDFAACHTHEIDECANVKFVDRIELTADKGFDNRPHDGDQHAALHAKEQSK"
     gene            9756..>10242
                     /locus_tag="VFB80_02215"
     CDS             9756..>10242
                     /locus_tag="VFB80_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HZN32596.1"
                     /translation="MIVVTSLKGSGPGSLQEACAAEGPRTIVFATSGVIERDVVIEHG
                     ELTILGQTAPGAGITIAGSLKTRYRARQPIDDLVVRFLRVRSANARGASGDTLQFSEN
                     KRVILDHVSAAWGTDETVDIFGAQDVTLQWCSIEESQTTGHPEGPHNYGLINGPQGAR
                     VS"
BASE COUNT         1764 a         3334 c         3243 g         1901 t
ORIGIN      
        1 cgatcgccga ccgggccggc aaccgtcgcg tgctgatcct cgtcacgctg ctgatcgccg
       61 gcggtccgct ggcggccctg gtttgtatct acgcgcccga atttggcaag cgggcgttcc
      121 cgctggtctt tacgctcgtc ggcctgacgc ccgtcgcgca aaagacgttc aacaactaca
      181 cgctcgaaat cacgaccccg gagaaccatc cccgctatct cagcactctc agcctgagca
      241 tggccgctcc catctgcgcg tcgccgctcg tcggctacgc cattgagctg gtcggcttcg
      301 aggtcgtcta tctgggcgtc gtcggcttgc tgctcgtcgg ctggctgatg agcttcggcc
      361 tgaccgaacc ccgcgccggc ggccggccgg tggtgctcgc cgaggactcc atcacggagt
      421 gatgtcgaat cgaatgggac tgatgagact tagggaaccg atgggccggc gggcttctgc
      481 tagcgtgcgg ttttccgtcc gaaaccttac gatcccttca gggtttttca gccgtctcat
      541 cgaacgagga gggggaacca tggacgggtc gattgacgaa aggcggcgcg cggagctgct
      601 ctcgctttgg aagtccaatc cccatcgggt cttgtcgctc taccggcagg tggtcgattt
      661 ggacgagctt gcaccgctgc ccgccgggac aaccgtcacc catttgatag acgtcatcct
      721 cgaaaaggct gcttgcgacg actcgttgtc tctgcagcca taggcgccgg cggctggcac
      781 cgaacttgca ctctgccctt acgcgccaca agaatggaag cgcctggagg gcagggctat
      841 gcttcccgac tccacaccgc cgtgtccggc ctgcggcgag ccaaacgccg cgacgcccgc
      901 ctggcaactg gtcgatgaag aaggacgcga gttgctccgt tgcggctgcg gccacgtctt
      961 tgcggtggag cccgcgcaaa atctgagcgt caaagaagtg atcgaccgtg caaggacggc
     1021 gcagttggcc tttcgcctgg aataccaatt gcatgaggat ctgcatcggg aattcgaaca
     1081 actgaaacgg cgcttccgtc acggccgtgg ccgtggcatc acaccgcctg cagactgaac
     1141 cgattcaagg ctttccggcc gctcgctcgt cgccgctcgg cgtataagcg ggcggcacat
     1201 agatgttgag cgtgaccagt tgccgcttgc ggctggtgtt ttgaatcagg tgcgtgtcgc
     1261 cgcgctcgat gagcagcaag ctgccttccc gcaatcgatg acgccgtcgg ttgatggtcg
     1321 cccggcccgc tcccgagacg acgaacagcc actggtccgc tccacgatgc cggttgtctg
     1381 cgccgccctc ttctgcgccc ggcgcaagaa ccatctcagc tgcctgggag cggcggttgc
     1441 caatgagaac gccaaacgta ctgcgaaacc gcactcgatg acttttcatg tcccttattt
     1501 gccgggagtc ctgtgtggcg gggcccaaag cggggtatca gatttgcacg aacgctgctt
     1561 accggctaat agaggtcgcc ccgtgcccgc aaaacccgag ccaatcgtgc gcatggccgt
     1621 tcgcggctgt ctggttcgct gtgcggacag cgacactccg ctgacgtctt tggccgaatt
     1681 cgccgaggaa ttacggcgct tgggctggga taaaaagagc attcgcgacg tggaatttga
     1741 ggtgattcag gtcctgggcc gacgcgacgg ccgcgaccgc cgcaaccagc accggcaaaa
     1801 aaactctgat agcgcttgac cgttcgcgac agcggtgcga cggcatgtca actaactggc
     1861 cggctgcttg tcgccctcca tggccagcag aatcagggcc gggcggtgat cggagccgct
     1921 aatccggcgg gcgttcagcc gaatctgctt cattcccacg cccgcaaact cttgccggat
     1981 ttcgaaatcc tcaaaatggc tgtcgctggg cagcacgtct tccagcaggt tccgcagccg
     2041 tgggttgttc caagcgccag cgcccagctc gtacagcagg cggccgcgga cgtcgtccag
     2101 cgtgactccg aacacgcggc agaacgcggg gttcaccgtc tgcacgcgca gtttgccgtc
     2161 gagcacgagc agcggctcgc ggaccgtgtt gaatacggcc tcggcatatt cgcgggcctc
     2221 ttgggcctgc agttcgtggc ggcggatttc atcgacgtcg aacaaagtca gcaccgcgcc
     2281 gtcgatgcgg ttatcgacgc tcttgtaggg gcggatggtg agcgatagcg tccgcccctg
     2341 cgagtccagc acgtcccgct gctggaccga aatcgtgtcg atcacctcca ggatcagctt
     2401 ctccaactcg ggaccgttga tgttgggctt gatctggctg atcggccggc cgatgtcgct
     2461 gggaatgaga ttgaggatcc gctcggccat cggcgtaaac cgcctgatcc gcaagtcgcc
     2521 ggcgacgatc acgatggcga tctgcacgct gttaatcagg ttgatgagat cgctgttgat
     2581 gcgggccagc tcgtcgttgc ggaagtgcag ctcctcgttg acggtgttca gctcctcgtt
     2641 ggtgctctgc agctcttcct tggcagtgtc gagctcctgg ttggtgctct gcagttcctc
     2701 gttgctggaa agaatctcct cgttggccga ctgcagctcc tcgttggccg cttccaggtc
     2761 ttgaatgacc gactgcaagt actcgcggct ggcgaccagc tctttctgca agcgctccac
     2821 ctcttcggcc tgtttgccgg tgcccttttt tcctactgag gcgatcttca gcgggggagc
     2881 ggtcgatttg gcgacgccct ggaacaggat caggtaatgc cggccttcgg cgctgggcgt
     2941 cagcggcact acgtccacgt tgacgtttac gaagtctccg ttggtcttga cccgcagtcc
     3001 ttcgcggcgc accggcgcgt ccttcttctt ggccgtttgc aaagccgtgc gcaagccgtg
     3061 cagcaggccc tcgcggcaca tcttgagcag gttcaggctg gcgtcgcccg gcgccggctc
     3121 caggaaccgc cccgtctggc cgcggaactg catgatccgc aggtcgtcgt tgacgatcac
     3181 gcccggcggc gaatagcggt ccaggatcag ccggttggcc tcgctctgca cgttgccgcc
     3241 gccgcgggct tcctcgccgg cccgcgcagc ccggccgaag gtcggccgcg tgtaaggcac
     3301 cgtgaattgc acgttctgcg gcgtctcacc cagcttcttg cgataaatgt ggtgcttctt
     3361 gtcgcatacc gcgaacagtt cgccgtgcgg accgattgtc tcggcgctcc ccagcatcaa
     3421 atagccggtc ggcttgaggg cgtagtggaa cacgccgatc agccggtgct gcagcggctg
     3481 gcccaggtag atgagcacgt tccggcagac gatcaggtcg agcttcgaaa acggtggatc
     3541 gcgcgtcagg tcctgccggg caaagacgca caaatcacgg atcggcttgt tgatctggta
     3601 ctggccgtcg gtcttcgtga agaaccgccg cagccgctcg ggggacagct cctcgacgac
     3661 gttgggcggg taaactcccg cgcgggcctg ctcgatcgcc gtctcgctga tgtccgtggc
     3721 gaaaatctgg atcgggacgc gctcgttgcg ctcctcgacg tgctccatga tcgtcatggc
     3781 caccgagtag gcttcttcgc ccgtcgagca ggccgggacc cagatgcgca gcggggcctc
     3841 gccttgccgg gcggccagga tcgcgggaat cgtctccgtg gccaattgcc tgaacgaatc
     3901 cggatcccga aagaaccgcg tgacgtggat caggatgtcc tggtagaggt tgttggcttc
     3961 ggcctggttt tcgcgcagaa ccgagacgta ttgctccagc gcgccgacct tgtgcagcgc
     4021 catccgccgc tgaatccgcc gctgcacggt cggcagcttg tattgtccga aatcgacgcc
     4081 gctgacccgc cgcagaatct cgaagatctg ctggaggtgc tccggatcga gcagggcggc
     4141 ctcggcggcg cggcgcgatt cagccggcga ctccgcccgg ttgcgggcga tgcgcgccag
     4201 ctcctgggcc agttccgcgg ggctcagcgc gtattcgatc ttgccggtgg aaatgaccgc
     4261 ctgcggcatg cccggctgct tggccgtcgc cggatcctgg gccagcgtcg tgccgccagc
     4321 agccttgatg gcgtgcatcc cctcgacgcc gtccgagccc gtgccggaga ggatcacgcc
     4381 gaccgcgtcg ctgccgtagg ccttggccag cgactggaag aagtggtcga tcggcaagta
     4441 ttgcgtgcgg tcggtgggcc gcggctggat gattagccgg ccgttttgca cctccagtcc
     4501 tcgattgggc ggaatgacat agacgtgatc cggctccaga cgcgagtcct ccgtgggctg
     4561 gatgacgggc aggctggtcg aagaggccag cagcgaggcg agcgcgcttt cgtggtgcgg
     4621 ggccatgtgc tgcacgaaga ccagggccag tccggtgtct gccggcaggg ccctgagcaa
     4681 ctggctgaac gcctccaggc cgcccgccga cgcgccgacg ccgacaaccg tcggagtgaa
     4741 ctcaagatca tcgtcggctg cactgggcgc ggtgcgaagc cgggcgggcg agcggccttg
     4801 cactaggcct tttttcgagc ttttcttgcc gcctgccatg ccagtccctc ggccaaccgt
     4861 cgttcgttgc tgctacacgc gcgagcttaa cgcgtcggcc tgccattcgc caccgggctc
     4921 aacgacccac tttcgactgc catctgcgcg attccgcgcc gtcaaataga cgagcgtatt
     4981 acaaaatggt gtgctggtgt tgaagttggg ccgacttgtg gtccaatcga actttcgttg
     5041 ggggacgatc gatgagccgg gagatggaag acttctggat gcacatccac cggctctcgt
     5101 cggcactcga cgacgccggc atcacccgcg acgagcgcgt gcggcacctg ctgcagcaac
     5161 ttgaacagtt gccgccgctg gttcgccgcg agctgttgct cgaattgacg caggtcgcgg
     5221 ctgacattct ggatctgcag ccgctggccg tggccgcgat gaacaaactc gaagaaccgg
     5281 ccgtgcagtc gcgacaaatg agctaaaggg gctgcacgtc gctcgtcgct tcggatcggg
     5341 caggcggcgg ggcctcaccg gcaaggcccg cctcggcagg cgacggcccg aacgatgtga
     5401 ttgacctgga cgttctggat cgcgttcagc ggcatgtcgg acagcgccgc ttcgtagggg
     5461 ctgcacgaga agacgatctc tttgtcgcgc ggctgcggct ttcttcgcgc gcgagtcggt
     5521 ttttgtttcg tagatttcat cgccccacac gggcgtcagg tcgccgcggg cacggctgcc
     5581 cgcagctctt cgctggcccg cgcgagcagc cggcggactt cgtcgtcgct cgtcggatcg
     5641 aagttgtcgt accacgcgcc caccgcatgg aacggctccg gcgtcgtcgc gcagatcgtc
     5701 tcgtcggcct ccgactcgaa ctccgcgcag gtctgcttcg ctccgaccgg aacggcgacg
     5761 acgatccttg ccggctgcag ctgccgcagc gccaacaccg cggcccgcat cgtggagccg
     5821 gtcgccaacc cgtcgtccac caggatcacc gtcttctcgc gaatttccgg cggaaggcgg
     5881 ctgccgcgat agagccgctc gcggcggtcc agctcgcgct gctcccgctg agccacttcg
     5941 gcgatccgcc gcggcggcac gctcagcatg cccagcagct cttcgttaag cacccgtgtg
     6001 ccgccggaag caatggcccc catggccagc tccggccggc cgggcacgcc cagcttgcgg
     6061 accaggaaga catcgagcgg tgcgtggagc gcccgcgcca cttcatagcc gacaggcacg
     6121 ccgccgcggg gcagcgcgag tacgagcacg tccggccggt ccgcgtagtt cgttagttcc
     6181 gcggccagcc gccgtccagc ctccacgcga ttgcgaaaca acatcggtcg actccttcgc
     6241 acgccccagg gacaggtctt aagcgcgcct tccttgcctc gcccaaagac gctatcgagg
     6301 tcatggcttc cgttccttta cgcgccgtgc tagctgcgga gctagcgtag cggcactgcc
     6361 ggctccgaaa ttcgctctaa ggcctcgccg ctcagccggt cgtcgcggtg cttcaccgcc
     6421 aggtcgccca gcgagacaat gccgaccagc cgcttctcgc ggttgatgac cggcagccgg
     6481 cggatctggt gcttctccat cagcccggcc acctcgtcga tgggctgatc gtcgaagcaa
     6541 tacacgacgc cgggcgtcat aacttccccg acggtcgtgg agcgcggctc gcagcactcg
     6601 gcggtggcac gtaccgttat gtcgcggtcg gtaatcatac cggccagctt gtcgtcctcc
     6661 ccacagatcg ggagcgagcc gacattgagc cgcttcatct tctccgcggc accggcaata
     6721 ctctcgccgg gcgacacaca ttgcacgccg cgggtcatca attcacgcac caacatggcc
     6781 agttccctcc ccgcactgcg gggcatccat tgctgagccg cgctgtgcag taaggaaata
     6841 atgcaggtaa cgtgccaatg catcgcggct gctaactcaa ctatcactaa cccgacgctt
     6901 aagcgagggg ttttctaagt tcgggccgca tcgataaagt cccctcgctc acgctaccgg
     6961 tttgtattag gagctctaag ggtgccgcga taaccgttgg gagcaaaccc atacgcctcc
     7021 aatggcaatt gaaatcgcat aacggaaact ctcccgcgtc ggaacttttt tcttcgctcg
     7081 tatttggcaa gtgcccgccg gaaacccctt ctgtacaact gcttagatcg acgaatgtgg
     7141 tccgcggatg ccatgacgaa cataagcatt tgagcttgcg ctcgcgagtt ttcgttatat
     7201 ctgcggaacg agttgcggct gtgttcagca gtcgctcaca gggaagaata gccgccttag
     7261 ccggacggtc agttccccag gcggcggcgt ccaaaatggc agtcgccgcg caaacggaac
     7321 gatactgcaa atagcgtacc attgttcact tatcaatggg cgagtgtcag tgtcgctcgc
     7381 cggacgcaga aggcgtcgtc acccgaaaag accaggtgca gctcgcgacc gtcggcactc
     7441 tgtccgaatt cggcgaactt ccagtcggcg acttgccgga cgtttagcgg gcccgaccgg
     7501 gccgcgtgat gttccggccg aagccggctg ggcaggaacg ggcgtgctcg tcggcagcgt
     7561 catgagctcg ccgctcagtt tctggcactt ccgcctccgg tggcacgtcg ccgaattgcc
     7621 agcagtccag gcaatgccac cggccgccgt ctgtcgagac gctgatcttc agccttacgc
     7681 cattgacccc ggtgaacgcg aattcttgag aaacaggaac catcggtcct cgaatcagtg
     7741 cggacgcagc ctgccgacaa aaactaccaa gcggtcacga gcacggtcca ggccgtccac
     7801 aacagagtca ccccgcaaag caacgctgcg atcaaaaaga tcggtcgcac gccgagcacg
     7861 aggccgctcg cgggcggaat gtgccggcgt gctgcgtatc ggttgtgtgc catgctcttt
     7921 ggtccgggcg agaaggtttt gaggcgcgcg ggcggcagtt tggccccgtc gtaggaacga
     7981 cgtcaggtgg aatagggcac ccgtcccgtc ccggcggtct gccggggccg cttgtccctg
     8041 gccaattgct cgcagtcgcg aattatcgtc cgcgcttgct cggcaatgct caccagatcg
     8101 acggccgacg cgatctcctt ggcatccatg tgctttccat ccgaatcaac aattgccagc
     8161 cgaagcatga caggcctctc taagaaagat gacttcgaac cgcaggtcag ttttcggttc
     8221 gtccgcttgt tcggcggaac ggacctcctc acgcggcgcg cgaggacgac acgcattgag
     8281 ccgccaccaa cacgtcagcc gcgccgtacg gccggagagc catgcggcgc tgcctcagcc
     8341 acggtgtgcc gccagcacgg cctcaacgac gatccatttc cgcccgtata ggaattcggg
     8401 gcgagggtcc aaaagaaggg cggacgccga gaatgccaaa tagaactacc ctaattattg
     8461 cacgatcctc accaaaagca agcccaaatc caccccgcga ggcggtagcc cgccgacgtt
     8521 aagcgtgacc cagtccagtt cccaccaggc ccgaggccaa cgccaatacc tcgcggccgt
     8581 caagttgtca tggcccgccg gctgcggccg atcagcagat agaggatcgc tccgagcgtg
     8641 ttcgtcagca aaatcaccag cacccagatc agccgctcgg tctggttcag cccgggattc
     8701 tggatcgcat cgatcagcgc ccagatccac agcaccaggc acagcaaccc gaggatcagc
     8761 atgaccagca tcatgccgcc gccgagagcg agcgcaccgc ccgccgcacc cacaccgtcg
     8821 cctgccgcct gagccaagag acagagtttc attgcttgtt ccttttgcaa ttcgtttaac
     8881 ccggggagcc ggaggctctt cgcggtagcc gccgtcgcct ccggcccggg ttcaacgagc
     8941 aatcgccgtg ccctcgccgc ggccaacttt cgcggcgagt ggtgctatga tgcggccatc
     9001 gcgacgcgta cttccgagga accaatgttc tccatatccg gcctggtcga atcgccccgc
     9061 tcgctcacgt ttgccgacct ggccgcgatt ggcgcggagc accaggtcgc ggatgtcagc
     9121 cggctggtgc cgggtcggaa gggagacgct gtcaggctgg cgggaatcct ggagctggtg
     9181 cggccgaagg aggccgcgaa gtggctgggc ctgcacgcgg cggccgataa ttttcacgcc
     9241 agcattccgc tcgacgccgt cgccgacaag gcgctcgtca tctaccgctt ggacgggcag
     9301 ccgctgcccg cgaaggccgg cggtccggtg cggttcttca ttcccgattt cgcggcctgc
     9361 cacacgcacg agattgacga gtgcgccaac gtgaagttcg tggaccggat cgagctgacg
     9421 gcggacaagg gcttcgacaa ccgcccgcac gacggcgacc agcacgcagc gctgcacgcc
     9481 aaggagcagt cgaaatgagc acgtgccctc ggtcccaagc tccgcttggg accgcaagtc
     9541 cgcgaagctc tgcttcgcga actggacacg aagcggagct tcggcggcgt gcgtgcccaa
     9601 gcggagcttg ggcacgagga gcttgggcac gagtcgttgc ggtcgcgacg atctgtgcgg
     9661 tcggttcatt atccgccctg gcagccgagc cgccgccggc gtttcccgga gccgagggct
     9721 ttggtgcggt cgccaagggc ggacgcggtg ggcgggtgat cgtcgtcacg agcctgaagg
     9781 gcagcgggcc gggcagcttg caggaagcct gcgcggcgga aggcccacgg acgattgttt
     9841 tcgccacgag cggcgtgatc gaacgggacg tcgtgatcga gcacggcgag ctgacgattc
     9901 tcggccagac cgcgccggga gcgggaatca cgatcgccgg cagcctgaag acgcgctacc
     9961 gcgcccggca gccgatcgac gacctggtgg tgcggttcct ccgcgtccgc tcggcgaatg
    10021 ctcgcggagc ctcgggcgac acgctgcagt tcagcgagaa caagcgggtg atcctcgatc
    10081 acgtctcggc cgcctggggc accgatgaga cggtcgatat cttcggcgcg caggacgtga
    10141 cgctgcagtg gtgcagcatc gaggagtcgc aaacgacggg gcatcccgag ggcccgcaca
    10201 actacgggct gatcaatggt ccgcagggcg cgcgggtctc ga
//
LOCUS       DASRJB010000079         6973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1151675, whole genome shotgun sequence.
ACCESSION   DASRJB010000079 DASRJB010000000
VERSION     DASRJB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151675"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFB80_02220"
     CDS             complement(<1..524)
                     /locus_tag="VFB80_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32597.1"
                     /translation="MLSQHASSLLVLAGVGLAVYLLLRSSAKRRQEQADEDQRPLAKA
                     STAAADAPADVLRWQIELYETARDLKAEIDTKFAALQALALLARQESQRLETALARVA
                     EVTTPHARHSLAAIESLGDSAQLDNRERLPAVAAQMPTHNDLRGALFDDNRDLVAVAN
                     LADQGLSAPEIARRL"
     gene            complement(641..1558)
                     /locus_tag="VFB80_02225"
     CDS             complement(641..1558)
                     /locus_tag="VFB80_02225"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN32598.1"
                     /translation="MSVTKVAPGTTRLGWIGTGVMGQSMCSHLLAKGFAVTVYNRSRA
                     KAQGLLDKGATWGASPKQVAQQSDVVFAIVGFPRDVREVFLGGEGALAGAQAGNVLVD
                     MTTSEPSLAVEIYTAAKSKGVAAIDAPVSGGDVGAREARLSIMIGGDRDAVDALSPCW
                     EAMGKTIIHQGGPGAGQHTKMVNQTLIASNMIGVCEALLYAYKAGLDLPTVLQSVAPG
                     AAGSWSLSNLGPRIIANNFDPGFFVEHFIKDMGIALDEAKRMNLALPGLALAHQLYIS
                     LKAKGHGRDGTHALTLALAELSGIDWRRR"
     gene            complement(1672..2199)
                     /locus_tag="VFB80_02230"
     CDS             complement(1672..2199)
                     /locus_tag="VFB80_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32599.1"
                     /translation="MPDEVEEFLRRVAQMRAQAEAQARAQQQRPKPQPAPPPPRPAAP
                     PPPARLVPARQEMVPLEPVEVEIVDAELAEIGDSVGRHVTQHLRGTEEIAEHTRHLGE
                     EVDQADDKLESHLHQVFDHQLGKLKKTASTTAAAPYDSPASDMSELLQMLRSPKSVRD
                     AIVMAEVLRRPDDRW"
     gene            complement(2296..3354)
                     /gene="floA"
                     /locus_tag="VFB80_02235"
     CDS             complement(2296..3354)
                     /gene="floA"
                     /locus_tag="VFB80_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120891.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HZN32600.1"
                     /translation="MSILLAQAELVPIIGIAAAIFVLFFMLIVVAVFANYFRLWIQSF
                     LTGAGITIWDLIGMTFRKVNKDVIVKSKIMAVQAGLGEEGEITSKALEAHALAGGNVP
                     LVIRSLIAAAKAKIIRLSFREATAIDLAGRNVLEAVQTSVYPKVIDCPGRGSGTLDAV
                     ARNGIQLKVRARVTVRANLQRVIGGATEETIIARVGEGIVSAIGKADDHMQVLENPDQ
                     IAAEVLGRQLDANTAFEIVSIDIADIDVGENVGARLRADQAEADTRVARARAEGRRAA
                     AVASEQEQYARIEESRAKLVEAEAEVPKAMADSFRTGRLGILDYYKLRNVQADTDMRR
                     AIAGSGAAGGSAGQATRQ"
     gene            3594..4094
                     /locus_tag="VFB80_02240"
     CDS             3594..4094
                     /locus_tag="VFB80_02240"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN32601.1"
                     /translation="MQFKQKITPCLWFDTQAEEAANFYVGIFENSRITTIARYGKAGT
                     EVHGKPAGSVMTVAFELEGLPFTALNGGPIFKFNEAISLQIYCQSQEEVDHFWNKLSA
                     GGDPKSQQCGWLKDKFGVSWQVVPTALLDMITSPDAARSQRAFGAMMQMKKLDIAALQ
                     RAFDGG"
     gene            complement(4145..4585)
                     /locus_tag="VFB80_02245"
     CDS             complement(4145..4585)
                     /locus_tag="VFB80_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32602.1"
                     /translation="MWHRAMVRLVSLGCLLLIAGCSLPQAQNTRLLKSWTAKCKEASD
                     LLATVKDAPSAKAASPKIIKLMKELDKLNEQLDESYDPSDVSLVEMPGTTKHLGEGIG
                     EMQRLMQESVRIGQDRELRAALGEAWQYLPAAPLVDAEGNLIQG"
     gene            complement(4677..5117)
                     /locus_tag="VFB80_02250"
     CDS             complement(4677..5117)
                     /locus_tag="VFB80_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN32603.1"
                     /translation="MTRTLQPWTNRLPRFFETEFPKWMGEVFGPEMEFGREERFLPAA
                     NLSETDKAFELAVELPGMKPENVKVEMHDGNLVVTGDKHEEQEEQGKTFHRIERRSGS
                     FRRVFALPAAVDENAIDAHFAHGVLKVTLPKTPEAAPKKIEIKG"
     gene            complement(5303..5782)
                     /locus_tag="VFB80_02255"
     CDS             complement(5303..5782)
                     /locus_tag="VFB80_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32604.1"
                     /translation="MNVTLPGFVLGVLLASAGPALADEQQPVKPPAVPPLPGVALPPG
                     YVWVLEGGLQPRVEGRDQPPTWLAEKLAKTKVPTMKEARQIAAEKVRPTAHIYDAITL
                     TSVVGIFELGRKVAKFGEEGDFVWVVRFVRKGGVTQELSISSSTGEVQMLLPAKVEE"
     gene            complement(5862..6677)
                     /locus_tag="VFB80_02260"
     CDS             complement(5862..6677)
                     /locus_tag="VFB80_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32605.1"
                     /translation="MQLHLACVLAAVALGQAPQGKTRLDAEAERKRQLEEFAADAVKY
                     EVALATPQPARLELLAQPVLNWDGSAFVWLKEGRPEVIGAFWKTLDARTGKLLYGHAF
                     HSLSDQPITATYSGQTIWSPKAAGLEFRPVEGAEAVAEDSRRRLVQLRSLSRDFSAVQ
                     WKGARSQLRMLTQPIYRYEPRSGACKDGAIFAFTNTEFGTDPDALLILEARQVEGKLR
                     WEYAFVRFHYAELSGNHQDQEVWRVKDTWQERKRHKFGADPGRENVYYSVILP"
BASE COUNT         1148 a         2338 c         2181 g         1306 t
ORIGIN      
        1 aggcggcggg cgatttcggg ggcggacagg ccctggtcgg cgaggttggc gacggcgacg
       61 aggtcgcggt tgtcgtcaaa gagtgcgccg cggaggtcgt tgtgcgtggg catctgcgct
      121 gcgaccgctg gcagccgctc ccggttgtcg agctgcgcgg aatcgcccag gctttcgatg
      181 gcggcgagcg agtggcgcgc atggggcgtg gtcacctccg cgacgcgagc cagggcggtc
      241 tccaggcgct gcgattcctg ccttgcgaga agggccaggg cctgcaaggc tgcgaacttc
      301 gtgtcgatct cggccttcag atcgcgggcc gtttcgtaga gctcgatctg ccagcgcagt
      361 acatccgccg gcgcgtcggc cgcggcggtg ctggccttcg ccaagggccg ctgatcctcg
      421 tcggcttgct cttgacgccg cttggccgac gagcggagca gcagatacac cgcgaggccc
      481 acgccggcga gcacgagcag cgagctagcg tgttgtgaca gcacaaaaac tccaagaacg
      541 aaggatcaga agaaatccaa gaaccaaaga atcgacgacc caaataaggt cctattggtt
      601 cttcggttct ttggttcttc ggttcttggg ttttacccgg ctaccgccgg cgccaatcga
      661 tccccgacaa ttccgccagg gccagcgtca gggcgtgcgt gccgtcgcgg ccgtggccct
      721 tggctttcag cgaaatgtag agctggtggg ccaaggcgag gccggggagg gcgaggttca
      781 tccgtttggc ttcgtcgagg gcgatgccca tgtccttgat gaagtgctcg acgaagaagc
      841 ccggatcgaa attattggcg atgatccgcg gcccgaggtt cgagagcgac cagctgcccg
      901 ccgcaccggg ggcgaccgac tggagcacgg tcggcaggtc gaggccggcc ttgtaggcgt
      961 acagcagggc ctcgcagacg ccaatcatgt tcgaggcgat cagcgtctgg ttgaccatct
     1021 tcgtgtgctg accggccccc gggccgccct ggtggatgat cgtcttgccc atcgcttccc
     1081 agcagggcga cagggcgtcc accgcgtcgc ggtcgccgcc gatcatgatc gacagccggg
     1141 cctcgcgagc acccacgtcg ccgcccgaga ccggcgcgtc gatcgcggcg acccctttgg
     1201 atttggcggc ggtgtaaatc tcgacggcca gcgacggctc gctggtcgtc atgtccacaa
     1261 gcacgttgcc cgcctgcgcc ccggccagcg cgccttcgcc gcccaggaag acctcgcgaa
     1321 cgtcgcgagg gaagccgaca atggcaaaca cgacgtcgct ctgctgggcc acctgcttgg
     1381 gcgatgcccc ccaggtcgca cccttgtcca agagcccctg ggcctttgcc cgcgagcggt
     1441 tataaaccgt gacggcaaag cccttggcca gcaggtgcga gcacatgctc tgccccatca
     1501 cgccggtccc gatccatccc agccgcgtgg tgccgggggc gactttcgta acggacatgg
     1561 caagctacct cgtacgagtc aatggtgcgg ctcagtgtaa tcaaagccaa agaaccgaag
     1621 taccaaagtc aatgggttct ttggttcttt ggatcttcgg ttcttgacct gctaccaccg
     1681 atcgtccggc cggcggagca cttcggccat cacgattgcg tcgcggaccg atttcggcga
     1741 gcggagcatc tgaagcaact cgctcatgtc ggacgcaggg gagtcgtaag gcgccgcggc
     1801 ggtggtgctg gcggtcttct tcagcttgcc gagctggtgg tcgaagacct ggtgaagatg
     1861 cgattccagc ttgtcgtcgg cctgatcgac ttcttcgccc aagtgccgcg tgtgctcagc
     1921 aatctcctcg gtcccgcgga ggtgctgcgt gacgtgccgg ccgacggagt cgccgatctc
     1981 ggccaactcg gcatcgacga tttcgacttc gaccggctcc agcggaacca tctcctgccg
     2041 cgcgggaacc aggcgcgctg gcggcggcgg agcggccggc cgcggcggag gcggcgcggg
     2101 ctgcggcttg ggacgctgct gctgggcccg cgcctgggct tccgcctggg cccgcatctg
     2161 ggcgacgcgg cggaggaatt cttcgacttc gtcaggcatg ggtcgtgctg gtggctagtg
     2221 ctggtggcta gtgaaaggaa aactgattct ggcactagcc actagcacta gccactagca
     2281 ccggttacta gcacgttatt gcctcgtcgc ctggccggcg ctgccgccgg ctgctccgct
     2341 gccggcgatg gcgcggcgca tgtcggtgtc ggcctgcacg ttacggagct tgtaatagtc
     2401 cagaatgccc agccggccgg tccggaacga atcggccatc gccttgggca cttcggcctc
     2461 ggcctcgacc agcttggctc ggctctcttc gatgcgggcg tactgctcct gctcggaagc
     2521 cacggcggcg gcccgccggc cttcggcccg ggcgcgggcc acgcgcgtat cggcctcggc
     2581 ctggtcggcc cgcaagcgag cgccgacgtt ttcgcccacg tcgatgtcgg cgatgtcgat
     2641 cgagacgatc tcgaacgcgg tgttggcgtc gagttgccgg ccgagcacct cggcggcgat
     2701 ctggtcgggg ttctccagca cctgcatgtg gtcgtcggcc ttgccgatcg cgctcacgat
     2761 gccctcgccg acgcgggcga tgatcgtctc ctccgtagcg ccgccgatga cccgctgcaa
     2821 gttggcccgc accgtcacgc gggcccgcac cttgagctga atgccgttgc gggccaccgc
     2881 gtcgagcgtg ccgctgccgc ggcccgggca gtcgatgacc ttgggataga cgctcgtctg
     2941 cacggcctcg agcacgttgc ggccggccag gtcgatcgcc gtcgcctcgc ggaacgagag
     3001 gcgaatgatc ttggccttgg cggcggcgat cagcgatcgg atcaccagcg gcacgttgcc
     3061 gcccgccagg gcgtgggcct ccagcgcttt gctggtaatc tcgccttcct cgcccaggcc
     3121 ggcctggacg gccatgatct tgctcttgac gatgacgtcc ttgtttacct tgcggaaggt
     3181 catcccgatc aggtcccaga tcgtgattcc cgcgccggtc aggaacgact gaatccacag
     3241 ccggaagtag ttggcaaaaa ccgccaccac gatcagcatg aagaacaaga cgaaaatggc
     3301 cgccgcgatc ccgatgatcg ggaccaactc ggcctgggcg agcagtatgg acatggcaga
     3361 atccatcggg ggaatcgtgc ggggattttc cgtccccaga gcttccggga ccagcggcag
     3421 ccacggccgc gccgatccat gcacgttcgt atcccactcc cagcagattg tcaagcaagc
     3481 gagtcaggcc ggccacgtcg ccaacttttt tggcgttctg tcgatttgga ctttcggcat
     3541 acgactagct gtagatcgtt cgtctcccag ctgcgttttc caggagagcc gccatgcagt
     3601 tcaagcagaa gatcactccc tgtctgtggt tcgacacgca ggccgaagag gccgccaact
     3661 tttatgttgg aattttcgag aattcacgga tcaccacgat cgcccgttac ggcaaggccg
     3721 gcaccgaggt tcacggcaaa cccgccggct cggtgatgac cgtcgctttc gagctggagg
     3781 ggctgccctt caccgccctc aacggcgggc cgatcttcaa gttcaacgag gccatctcgc
     3841 ttcagatcta ttgccagtcg caggaagagg tggatcactt ctggaacaag ctctccgcag
     3901 gcggcgatcc caaatcccag cagtgcggct ggctgaagga caagttcggc gtttcctggc
     3961 aggtcgttcc cacagccctg ctcgacatga tcacgtcccc cgacgccgcc agatcccagc
     4021 gggcgtttgg cgccatgatg caaatgaaga agctcgacat cgcggcgctg caaagggcct
     4081 tcgacggcgg gtagcgtggg ctgtgcccgc cgcacgagca ccggtctggt gggcagagcc
     4141 cacgtcatcc ctgaatcaga ttcccctcgg cgtccacgag cggcgcggca ggcagatact
     4201 gccatgcctc gcccagggcc gcgcgcagct cgcggtcctg gccgatccgc acgctttcct
     4261 gcatcagccg ctgcatttcg ccgattcctt cgccaaggtg cttcgtggtg ccgggcattt
     4321 cgaccaggct gacgtcgctc ggatcgtacg attcgtcgag ttgctcgttc agcttatcca
     4381 gctctttcat gagcttgatg atcttcggac ttgcggcctt ggccgagggc gcatccttga
     4441 ccgtcgccag caagtcggac gcctccttgc acttcgcggt ccaggacttc agcagccgcg
     4501 tattctgggc ctgcggcagc gagcagccgg cgatgagcag cagacagccg aggctgacga
     4561 gtcgaaccat tgcgcgatgc cacatgagat gttcctcacg aggcccgcat tctagccgcg
     4621 caatactcaa cttgcagcct ggtctcgcgt acggcgacgg cgaccacctc ctgcaattag
     4681 cccttgatct cgatcttctt gggcgcggcc tccggcgtct tcggcagggt gaccttcagc
     4741 acgccgtggg caaagtgggc gtcgattgcg ttttcatcga cggcggcggg caatgcgaag
     4801 acgcggcgaa agctgccgct gcgccgctcg atccggtgga acgtcttgcc ctgctcctcc
     4861 tgctcctcgt gcttgtcgcc cgtaacgacg aggtttccgt cgtgcatctc gaccttcacg
     4921 ttctcgggct tcatcccggg caattcgacc gccagctcaa acgccttgtc ggtctcgctc
     4981 aagttcgccg cgggcaggaa ccgctcctcg cggccgaact ccatctcggg gccaaacact
     5041 tctcccatcc acttggggaa ctccgtttca aagaaccgcg gcaggcggtt ggtccagggt
     5101 tgcaatgtgc gcgtcatgac ttcgctcctc aatttcgagt cacgccgcgc cggccccggc
     5161 gcgcgccgca gaaacgccgg cgcggcccaa caatatcgat ggctcttctc ttcctacgcc
     5221 cgtcacccgc cgccggatcg ccagaaatag ggatgcctct agggaatgcc ctctgtggca
     5281 ttccggcacg ccgcaaatcc ccttattcct cgactttcgc cggcagcagc atctgcactt
     5341 cgccggtcga gctgctgatc gacagctcct gcgtgacgcc gcccttgcgg acgaaccgca
     5401 cgacccagac gaagtcgccc tcctcgccga atttcgccac cttccggccc aattcgaaga
     5461 tgcccacgac gcttgtcaac gtaatcgcat cgtaaatgtg agccgtcggc cgcacctttt
     5521 cggcggcgat ctgccgtgct tctttcatcg tcggcacttt tgtcttggcc agcttctccg
     5581 ccagccacgt cggcggctga tctcgccctt ccacgcgcgg ctgcaggccg ccctccagaa
     5641 cccacacgta tcccggcggc agcgccaccc ccggcagcgg cggcaccgct ggcggcttga
     5701 ccggctgctg ctcgtcggcc agagccggcc cagccgacgc cagcaaaact cccagcacga
     5761 acccgggcaa agtgacattc atggtcggcc tccctggacc atagattacg acgcgtttcg
     5821 tcccgctgcg caacaatcgt gtaactattg cgtcatcgtg cctacggcag gatcaccgag
     5881 taatagacgt tctcgcggcc ggggtccgcg ccaaacttgt gccgtttgcg ttcttgccag
     5941 gtgtctttga cccgccagac ttcctggtcc tggtgattgc cgctgagctc ggcgtaatga
     6001 aatcgcacga aggcgtactc ccaccgcagc ttgccctcca cctgccgggc ctccaggatg
     6061 agcagggcgt ccgggtcggt gccgaactcg gtattcgtga aggcgaagat cgcgccgtcc
     6121 ttgcaagcgc ccgagcgggg ctcgtagcga tagatcggct gcgtcagcat gcggagctgc
     6181 gagcgggccc ctttccactg aaccgcggag aagtcgcggg aaagcgaccg cagctgaacc
     6241 agccgccgcc gcgaatcctc cgcgacggct tcggctcctt ccacgggccg aaattccagg
     6301 cccgcggcct tcggcgacca gatcgtctgg ccgctatagg tcgccgtgat cggctgatcg
     6361 ctgagcgaat gaaaggcgtg gccgtagagc agtttgccag tgcgggcgtc gagcgttttc
     6421 caaaacgcgc cgatcacttc cggccgcccc tccttgagcc acacaaacgc cgaaccatcc
     6481 caattcagca ccggctgcgc aagcaattcc agccgcgccg gctgcggcgt cgcgagcgcg
     6541 acctcatact tcaccgcatc cgccgcgaac tcctccaatt gccgcttccg ctcggcctcc
     6601 gcgtccagcc gcgtcttgcc ctgtggcgct tgcccaagcg ccactgctgc caggacacag
     6661 gccaaatgaa gttgcatcgc cgcgtcctcc cagctgtcga ggtccggaag cgcctctgcg
     6721 caggataaga cactatgtct ggaactgcaa gcgacgcagg gatgcggtgg caatcgcttg
     6781 tgactgctgc cccgcgttct tatagtacga gcgctgccct cgccccaatt tcaccgccgc
     6841 gccatccctg gaggacaagt ccgtgaaaac caccctgacg ctggtcgttg ccgctggcct
     6901 gagtttgctg gttgggctgg tccgtgcgga ggacaagccg cacgcggctg ccgccgggct
     6961 ggagcgcttc aag
//
LOCUS       DASRJB010000080         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3410057, whole genome shotgun sequence.
ACCESSION   DASRJB010000080 DASRJB010000000
VERSION     DASRJB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410057"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VFB80_02265"
     CDS             complement(<1..1479)
                     /locus_tag="VFB80_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZN32606.1"
                     /translation="MVPLYQQHLAALRSAFPAPVSDPVHDGYVVFSLLRTLDRVDALK
                     SQAPILGTPREPDYAAAAEARLGDGPQTLEAVIPQLVGVLDGMLITGHPRSQVNVVAH
                     PSIASIIGVILPSMYNPNLCSEESGRGFSAAEVRVASIASRLVGYDPARAGGVFTFGG
                     TGTLLYGIKIGLEKALPGTFAKGLARHSVAGSRDGVAGHRAVILCSQQGHYACSSVAA
                     WLGLGKDNVIRVPSTRDNAIDIAACEAEAREQLAAGNTIAAIVATMGTTDAFGIDDLA
                     GLAAMRDRLAAEFKLPYKPHLHADAVIGWAWSVFSDYSFAENSLGFRGRTVRALAAAV
                     SRIRHLGLADSLGIDFHKTGFAPYISSLFLLRDRADFAAVVRQRETMPYLYHTGEYHP
                     GMFTLETSRSATGVMAALANLLLLGKDGFRTLIGHAVEMAEVLRELIISRPELSVLND
                     DNYGPVTLFRAYPPGVDTFQAVERERSDPSYAGELAQHNELNR"
     gene            complement(1582..>2153)
                     /locus_tag="VFB80_02270"
     CDS             complement(1582..>2153)
                     /locus_tag="VFB80_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32607.1"
                     /translation="RRGRGEGRGESSEMLPPRRQEEAPPPELESEFDLDRDEELDLER
                     ALDEEFPDQPRLPEERESPPAGDREEREPRRRRRGGRRRGGRDRDEAAAERSSGFGAP
                     EADEQRPAARPVQDSEPADQLDEHDDLDSAEGDEEHVSGVPTHKKIPTWEEAVGLLIE
                     SNMAGRASGPDRDPYRGRGRGGRGRGGRR"
BASE COUNT          332 a          741 c          725 g          355 t
ORIGIN      
        1 gcggttgagc tcgttgtgct gggccagctc gccggcgtag gacgggtcgc tccgttcgcg
       61 ctcgacggct tgaaaggtat cgacgccggg cggataggcg cgaaacagcg tgaccggtcc
      121 gtagttgtcg tcgttcagta cgctcagctc cggccggctg ataatcagct cgcggagcac
      181 ctcggccatc tcgaccgcgt ggccgatcag cgtgcggaag ccgtccttac cgagcagcag
      241 cagattcgct aaagccgcca tcacgccggt ggcactccgc gaggtctcca gcgtgaacat
      301 gccaggatga tactcgcccg tgtgatacag gtagggcatc gtctcgcgct gccgcacgac
      361 ggcggcgaaa tcggcccggt cgcggagcag aaacagcgac gaaatgtaag gagcgaatcc
      421 cgtcttgtga aagtcgatcc ccagcgaatc ggccagcccc aagtgccgga tgcggctgac
      481 ggccgcggcc agagcccgca cggtccggcc gcgaaaaccc agcgagtttt cggcaaaact
      541 gtagtcgctg aacaccgacc aggcccagcc gatgaccgcg tcggcgtgca ggtgcggctt
      601 gtacggcagc ttgaactccg cggccaggcg gtcgcgcatc gcggccaggc cggcgaggtc
      661 gtcgatcccg aaggcgtcgg tggtgcccat cgtggcgacg atcgctgcga tcgtgttgcc
      721 cgcggcgagc tgctcgcggg cctccgcctc gcaggctgcg atgtcgatcg cattgtcgcg
      781 cgtcgagggc acgcgaatga cgttgtcctt ccccaggccg agccacgctg cgacgctgga
      841 acaggcataa tgcccctgct gcgagcaaag gatgaccgct cggtggcccg cgactccgtc
      901 gcgggaaccc gccacggagt ggcgggctaa gcccttcgca aacgtgcccg gcagcgcttt
      961 ttccaggccg attttgatgc cgtacagcag cgtgccggtg ccgccaaagg tgaacacgcc
     1021 gccggcccgg gcgggatcgt agccgaccag ccggctggcg atcgaggcga cgcggacctc
     1081 cgcggccgag aacccgcgac cgctttcctc gctgcacagg ttcgggttgt acatgctcgg
     1141 cagaatgacc ccgatgatgc tggcaatgga cggatgggcg acgacgttta cctggctccg
     1201 cgggtggccg gtgatgagca tgccgtccag cacgcccacg agctgcggaa tcaccgcctc
     1261 cagcgtctgc ggcccgtcgc ccagcctggc ctcggccgcg gcggcatagt ccggctcgcg
     1321 cggcgtgccg aggatcgggg cctgcgactt cagggcatcg acgcggtcca gcgttcgcag
     1381 cagcgaaaac acgacgtagc cgtcgtgcac cgggtccgag accggcgcgg ggaaagccga
     1441 acggagggcc gccagatgct gctgatagag aggaaccacg gaggaaagct ccaagaacca
     1501 aagaaccgaa gaaccaaaac tgtggctgtc aacctgtctt tggttctttg gttctttggt
     1561 tcttcggttc ttggtgtttt cctatcgccg cccgccgcgg cctcttcccc cgcgccccct
     1621 tccgcgataa gggtcgcggt cggggccgct ggcccgcccg gccatattgg actcgatcag
     1681 caggccgacg gcctcttccc aggtgggaat cttcttgtgc gtgggcacgc ctgagacgtg
     1741 ctcctcgtcg ccttcggccg aatccaaatc gtcgtgctcg tcgagctggt ccgccggctc
     1801 gctgtcctgg acgggccgag ccgcggggcg ctgctcatcc gcctctggtg cgccaaaacc
     1861 cgaggatcgc tcggccgcgg cctcgtcgcg gtcgcgcccc ccgcgccggc gaccgccccg
     1921 gcgacggcgg cgcggttcgc gctcttcgcg gtcgcctgcc ggcggcgatt cgcgttcctc
     1981 gggcagccgc ggctgatcgg gaaactcttc gtcgagcgcc cgctccaggt ccaattcctc
     2041 gtcgcgatcc agatcgaact cggattccag ctccggcggc ggcgcctctt cctggcggcg
     2101 gggcggcagc atctcgcttg actcgccgcg cccttcgccg cggccccggc ggc
//
LOCUS       DASRJB010000081        14111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2846372, whole genome shotgun sequence.
ACCESSION   DASRJB010000081 DASRJB010000000
VERSION     DASRJB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846372"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFB80_02275"
     CDS             complement(<1..831)
                     /locus_tag="VFB80_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN32608.1"
                     /translation="MNLIVGFVRNPVKVTVGVILVLLFGVLAMISMPMQLTPEVEIPT
                     LSIETVWPGASPQEVEREIIQEQEEQLKSVEGVRKISSESMDSMGRVTLEFPVGTDMG
                     EAMLKVNTKLAQVPDYPEDAKEPVINTSNAADRPIAWFILSQRVPPRADADQLVAQHP
                     ELKAEIDRAYIAKSEGLKMHRMRALAAKDPRVRAWLPDVGDVSTKRKFAEDFIEAALE
                     RVEGVSNSNVFGGQEEELQVVIDPERLAARGLTINDVRQALRSQNEDTSGGDYWEGKR
                     R"
     gene            complement(841..2145)
                     /locus_tag="VFB80_02280"
     CDS             complement(841..2145)
                     /locus_tag="VFB80_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN32609.1"
                     /translation="MPILTARRGGAIILAIALAAGGWLLLSTGQSPAQPPKGGGKFGP
                     PPAPKVLVTAARGDTIAEPRSFVGTVKPIRRSLVGSAVAGRVEEYLINDGDPVKAGQP
                     IAHLRRGVIQAELNVAKGDLQVREAELGELQKSFQEEVEQAQAKLANAQATLTYRENK
                     MQRSRALGPSVAKEIYEEDLSLYTQAASSAREAQAALRLLTHGAREMKTQQAVARVES
                     QKAEVARLQEQFDRHTMIAPFDGWVSAEHTEVGQWVMQGDPVAEIVELNEVDIEIAVV
                     EDFIGNLHTAVEGAVEVPALPKLHLTGRVAIINPQADARARTFPVKVRVQNQIESGQP
                     VLKAGMFARVTLPVGKPTACVLVPKDAVVLGGPTPVVFAVATAGGKSSVRPVSVTLGP
                     AQGTWISAIGELKEGDSVIVEGNERVRPGQEVRPEPKTVAYP"
     gene            complement(2189..2644)
                     /locus_tag="VFB80_02285"
     CDS             complement(2189..2644)
                     /locus_tag="VFB80_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN32610.1"
                     /translation="MLEYDFENSVGFWVCQASHAIQRDFNEELAPQGVTYRQAQVLGC
                     LALEGRLSQTDLAERMRIEPPTLVGILDRMEQGGWIRRDADKSDRRRKFIQPTLAAKP
                     VWSKIVAAAKRVRTRAARGLSAAQLAQLKKLLGLVQANLKQAAAQREAG"
     gene            2812..2895
                     /locus_tag="VFB80_02290"
     tRNA            2812..2895
                     /locus_tag="VFB80_02290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2846..2848,aa:Leu,seq:gag)
     gene            complement(2915..4321)
                     /locus_tag="VFB80_02295"
     CDS             complement(2915..4321)
                     /locus_tag="VFB80_02295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN32611.1"
                     /translation="MTSNSSSPPPLKIAYLTAGAAGMICGSCLHDNTLARALIAQGHD
                     VQLVPLYTPIRTDEEDVSGRRIFYGGVNMYLQQKLPLFRRLPKWLDRWLDQPWLINWA
                     SGRSVKIDPQQVADLTLSILRGTAGFQRKEVERLAEWLAGDLHPQVIVFSNILTAGCV
                     PEIKRRMNVPVVVTLQGDDIFLRGLPQPHQSQALAEIRRLAGQIDGFIANTRFYADAM
                     AEYLSLPRERIEVVPLGIDTRDFVSERSGVRVQGSEANGPPKIGYLARLAPEKGLHVL
                     AEAFIALRRMPGMENTRLQIGGWMGEKNRSYAEGVFARLREAGLGAAFEYIGEVDRRG
                     KLELLASLDVLAVPTTYREPKGLFVLEALAAGVPVVQPDHGAFPEVLAETGGGLLHRP
                     EDPQHLAERLHELLANDELRRALAAKGRDSVLVSRSAETMALSTAQALRITIEWHRSR
                     GESKRPEAAIAREDAAVT"
     gene            complement(4373..5125)
                     /locus_tag="VFB80_02300"
     CDS             complement(4373..5125)
                     /locus_tag="VFB80_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32612.1"
                     /translation="MQRRARLCMLYSPRPLESSSPDAAPPEILAAASRCPAARVSIMG
                     PLLTKWTIRLALACYVAYLAGWLTAKSERWPGTARWLWTGGCALFVVHVACAFHFYHG
                     WSHAAAWQTTADETRRMLGFAFGDGIYGSYLFLIAWVADVVWLWTGSRPAAAGSCATS
                     VPRPAWPWGQTPAWRVGVHVFLLFIAFNGAIVFESGPTRWFGMAACLALGFLAVRRTY
                     NALGSSAQRVELPRLQEISKDRRTEEPRQMVH"
     gene            5341..5772
                     /locus_tag="VFB80_02305"
     CDS             5341..5772
                     /locus_tag="VFB80_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZN32613.1"
                     /translation="MKRNEFLKQVMRTLVARRDALRTTLAGDAAVLRAGQDAGVGDEI
                     DAALASEQAEMRSQMASYESRELAQIDAALERIKAGSYGRCDNCGDPIASLRLKTLPY
                     ASECIGCARGAERRSARSVNRIAGYDIVPGPARDESLEEIG"
     gene            5889..6395
                     /locus_tag="VFB80_02310"
     CDS             5889..6395
                     /locus_tag="VFB80_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32614.1"
                     /translation="MVRSFTASLLLVALLAQAVPGQEPAKSSPTPEMIKKLQDDLTNV
                     KLIGSFTIAGKEDQTPKPEEYTISSALKLPDGDVWLIKARIKYGDKDATVPIPLEIKW
                     AGDTPVITLTNLEIPGLGTFSSRVVIYEGRYAGTWQHGKVGGHLFGKLEKATANDPAK
                     TEPKPADK"
     gene            6464..7123
                     /locus_tag="VFB80_02315"
     CDS             6464..7123
                     /locus_tag="VFB80_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZN32615.1"
                     /translation="MFELVDFVLQFDEHLDKLAGDFGVWTYAILFAIVFCETGLVVTP
                     ILPGDSLLFAVGAFCARPNQPLEIWTATILLTIAAILGDTVNYHIGKYLGPKVLSGKL
                     SRWLNPKYLERTQWFFDRYGGKTIVLARFVPIVRTFAPFVAGVGAMHYGRFLAYNIFG
                     GIAWVVICTQAGFWLGNIPVVRERFELVVLLIVAISLLPALIEYCLHRFGRKPAAEKA
                     L"
     gene            complement(7152..8207)
                     /locus_tag="VFB80_02320"
     CDS             complement(7152..8207)
                     /locus_tag="VFB80_02320"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZN32616.1"
                     /translation="MSEHVVIIGSGPAGWSAAIYASRANLHPLVFEGAETEDNRVAGT
                     LPLGQLNLTTEVENYPGFPSGHLHEYIRSAVAKDRVDTYLEPLHEHPHAVTGPELMNL
                     MRQQALNFGARIITDDVVSVDLSKQPYKLTTLEGKTHEALTIIIASGARANYLGLASE
                     EKYKNRGVSACAVCDGALPRFRNKPLVVVGGGDSAVEEATYLTKYASAVHMLMRRDVF
                     RASKIMQDRAQANPKIQIHYFREVDEVLGDEKSGVTGIRVRNNREGGTHDLAASGLFL
                     AIGHTPNTAFLRGQLELNAMGYIKYTTPLRTFTSKEGVYAAGDVADDYYRQAITSAGT
                     GCMAALDAERYLAAKGL"
     gene            complement(8277..9053)
                     /locus_tag="VFB80_02325"
     CDS             complement(8277..9053)
                     /locus_tag="VFB80_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZN32617.1"
                     /translation="MDLYDTHAHLSDEQIAADVAGVVERATAAGVSRILAVGTTAESS
                     RQCLELARRFAGARSSAGIHPNHASEAQPGDWDEIVRLSGEPEVVALGETGLDLYWKD
                     TPLAVQQDYFDRHIRLSQQSGLALVIHLRETAAEILAMLRQARERGPLRGVMHSFTGT
                     AQQAAEFLDVGMHISFAGMLTYKKSDDLRAVAATVPAERLLVETDSPYLSPEPFRGKR
                     PNEPARVVHTAQCLAQVRGVSIADLASQATANAIALFRRP"
     gene            complement(9068..12508)
                     /locus_tag="VFB80_02330"
     CDS             complement(9068..12508)
                     /locus_tag="VFB80_02330"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HZN32618.1"
                     /translation="MPKITKLLVANRSEIAIRVFRAAHELGIRTVAIYSHEDRYALHR
                     FKADEAYLVGKAGEPIRSYLNIANIIAVAKEHGIDGIHPGYGFMSENPELARACEQAG
                     ITFVGPHEAALTQLGDKTFARSIASRVGVPVLGGSSAIASAKEGLKIAGKLGYPVILK
                     AAKGGGGRGMRVVQSESEFAAAFEQAQRESLAAFGSPDVFLEKFITRASHIEVQILGD
                     QQGHLVHLFERDCSVQRRHQKVVEIAPAPNLDPSVRQALLDAAIKIGRAVKYENAGTV
                     EFLVDRDTNQHYFIEVNPRIQVEHTVTEEVTGIDLVKSQILVAQGVPLSDPEIGLPSQ
                     DAVQTTGYAVQCRVTTEDPTNNFLPDYGRIAHYRSASGMGIRLDAGTAFSGATVNPFY
                     DSLLVKVTARGRRFIDACARMERCLQEFRVRGVKTNIQFLIRLVTNPTFLEGQVTTRF
                     IDETPDLFALPRRRNRATRLLTYLGEIVVNGHPLVKGLPKAVRRAAAPVPACDLNAPP
                     PPGTRDKLQELGPKKFGEWILKQKPLLLTDTTFRDAHQSLLATRFRTRDLLNIADVYA
                     RNASGLFSLEMWGGATFDTALRFLKEDPWRRLTDLRERIPNILFQMLFRAGNAVGYAS
                     YPDNVVREFCKESAEAGMDIFRIFDSLNWVPNMRVAMEAVLKTGAICEPAICYTGDIL
                     NPQRSKYDLKYYVELAKELEKLGAHILAIKDMAGLCKPYAAQLLVKTLKQEIDIPIHF
                     HTHDTAGIQAGSILEAAEEKLDIADGAMSAMSGGTSQPNLNAVVEALRFTKRDTGLDG
                     HKLAEISDYWQAVREFYLPFDSNLLPATAELYSHEMPGGQYTNLFQQARALGLADRWQ
                     EVCRAYADANQLLGDIVKVTPSSKAVGDLALFMVANGLSAKDIAEGDRELAYPESVID
                     LLSGMMGQPPGGWPKGLQKRILKDRQPVKGRAGGSLPAADFDAALERVRGMIAREPNR
                     RDALSYLLYPKVYQEFAAHAKAYSDTSVLPTPVFFYGQEVGEEISVEIEEGKTLIIKF
                     LTVSEPHADGTRTVFFELNGQPRDVSVVDNSLEAAGNKRIKADPADPKQVGAAMPGMV
                     VTVVVKAGDKVTRGQKLFTLEAMKMETTVSSEVAGRVADVLVKPGSRIETGDLLLRIE
                     "
     gene            complement(12621..13814)
                     /locus_tag="VFB80_02335"
     CDS             complement(12621..13814)
                     /locus_tag="VFB80_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN32619.1"
                     /translation="MTPQCAGPPGLRFYPVYTIPDLTVGAIACRPSGPRFAAVLILLV
                     AAANLAGQETATRGTLLAPKAFRAAAAKVQPSLVRIEGFGGIAPGVGGGGYQAAGEGP
                     TTGIILSPDGYIITSTFNFLRKPPIITVVMEGGRRHVAQLLGRDETRKICLLKVDGLA
                     DLPVPQLAPRDELKVGQWAVAIGVGFGAQEPALSAGIISATSRISTKAIQTDANTSPA
                     NYGGPLVDLEGRVIGVCVPLSPGSREEAAGAEWYDSGIGFAIPLEGLSEIIGRLKAGE
                     TLKHAFLGVASEPFGEPPSGAEIKEVVPDSPAAKAGLEKGDKILKVGGAEIMDVTHLR
                     SILGRHFAGDRVEVLVRRGEDETIVTAELAVPPPPKPREAPMPMRAKPGEPQPMPPKP
                     PRPPM"
BASE COUNT         2333 a         4645 c         4561 g         2572 t
ORIGIN      
        1 tcgccgtttg ccttcccaat agtcgccgcc ggaggtgtcc tcgttctggc tgcggagggc
       61 ctgccgcacg tcgttgatcg tcagcccgcg ggccgccagc cgctccggat cgatgacgac
      121 ttgcagctcc tcttcctggc cgccgaagac gttagagttc gagactccct cgacgcgctc
      181 cagtgcggct tcgatgaagt cctcggcgaa cttgcgcttg gtggaaacgt cgccgacgtc
      241 ggggagccac gcccgcacgc gggggtcctt cgcggccagg gcccgcatcc ggtgcatctt
      301 tagcccctcg ctcttggcga tgtaggcccg gtcgatctcg gccttcagct cggggtgctg
      361 cgccacgagc tggtcggcat cggcgcgggg cggcacgcgc tggctcaaaa tgaaccaggc
      421 aatcggccgg tcggcggcgt tcgacgtgtt gatgaccggc tctttggcgt cctccggata
      481 gtcgggcacc tgggcgagct tggtgttgac cttgagcatc gcctcgccca tgtcggtgcc
      541 gaccgggaac tccagcgtga cgcggcccat cgagtccatg ctctccgacg agatcttgcg
      601 gaccccttcg acgctcttga gctgctcttc ctgctcctgg atgatctcgc gctcgacctc
      661 ctgcgggctg gccccgggcc agacagtctc gatcgacaac gtcggaatct ccacctccgg
      721 cgtaagctgc atcggcatcg agatcatcgc cagcacgccg aacagcaaca caagaatcac
      781 gccgaccgtg accttcaccg gattgcgcac gaagcctacg atcaaattca tggcgaacgc
      841 ctacgggtat gcgactgttt tcggttcggg gcggacctcc tgtccggggc ggacgcgttc
      901 gttgccttcg acgattaccg agtcgccttc cttcagctca ccgatcgcgc tgatccaggt
      961 gccttgagcc gggccgagcg tcacgcttac cggccgcacg ctgctcttgc cgcccgcggt
     1021 cgcgacggcg aacacgaccg gcgtcggccc gccgagcacg acggcgtcct tcggcacgag
     1081 cacgcaggcc gtcggcttgc ccacgggcag cgtgacgcgg gcgaacattc ctgccttcag
     1141 caccggctgc ccgctctcga tctggttttg cacgcggacc ttgacgggaa aagtgcgggc
     1201 ccgcgcgtcg gcttgcgggt tgatgatggc gacgcggccg gtaaggtgca gcttgggcag
     1261 cgccggcact tcgaccgcgc cttcgacggc ggtgtgcaag ttgccgatga agtcctcgac
     1321 cacggcgatc tcgatgtcca cttcgttcag ctcgacgatc tcggccaccg gatccccctg
     1381 catcacccat tggccgactt ccgtgtgctc ggccgagacc cagccatcga acggggcgat
     1441 catcgtgtgc cgatcgaatt gttcctgaag ccgtgctacc tctgctttct gcgactcgac
     1501 gcgggccacg gcctgctggg tcttcatctc ccgcgcgcca tgcgtgagca ggcggagggc
     1561 ggcctgggcc tcgcgagcac tcgacgcggc ctgcgtgtag agcgacaggt cctcttcgta
     1621 gatctccttc gccaccgacg ggcccagggc ccgcgaccgc tgcatcttgt tctcgcgata
     1681 ggtgagcgtg gcctgagcgt tggctagctt cgcctgcgcc tgctcgactt cctcctgaaa
     1741 cgacttttgc aactcgccca gttcggcctc gcggacttgc aggtccccct tggcgacgtt
     1801 caactccgcc tgaataacgc cgcgccgcaa gtgggcgatc ggctggccgg ccttcaccgg
     1861 gtcgccatca ttgatgaggt attcctcgac gcgaccggcc acggcgctgc cgacgaggct
     1921 gcggcggatg ggctttaccg tgcccacgaa cgagcggggc tcggcgatcg tgtcgccgcg
     1981 ggccgcagta accagcacct tgggagcggg cggcgggccg aactttccac cacccttcgg
     2041 aggctgggcc ggcgactgtc ccgtagacag cagcaaccat ccgcccgccg ccagggcaat
     2101 cgccagaatt atggccccgc cgcgccgggc tgtcagaatt ggcatggcaa gttccctagg
     2161 ttctttggtt cttcggtccc ttgccgccct agccggcttc ccgctgggcc gccgcttgct
     2221 tgagattcgc ttgcaccagg ccgagcagct ttttgagttg ggccaattga gccgcggaca
     2281 ggccccgcgc ggcgcgggtt cgcacccgtt tggcggcggc gacgatcttc gaccagacgg
     2341 gcttggcggc gagcgtgggc tgaatgaact tgcggcggcg gtcggacttg tcggcgtcgc
     2401 ggcgaatcca gcccccctgt tccatgcggt cgagaattcc taccagggtc ggcggctcaa
     2461 tccgcatccg ctcggccagg tcggtctgcg acaggcgccc ctcgagggcc aggcagccca
     2521 gcacctgggc ctgccgatag gtcacgccct gcggtgccag ctcttcgttg aagtcccgct
     2581 ggatcgcgtg cgacgcctgg cagacccaga agcccacgct gttttcaaag tcgtattcga
     2641 gcacgtgccc cttagcctcc aaatagttag actacgaatt attaggtcgc aatgtatcgt
     2701 acccgctagc ggcccgcaat caagctgccg gctgccccga acctgccgcg ccgcggccgt
     2761 gtctcttgac ggcagccggc ggcggcctac attagatgtt gccgttttcc cgcggtcgtg
     2821 gcggaattgg cagacgcacc agcttgaggg gctggcgccc gcaagggcgt ggaggttcaa
     2881 gccctctcga ccgcacttta gtccgtgtgc cgctttaagt tacggctgcg tcttctcggg
     2941 caatggccgc ctcaggccgc tttgactcac cgcggctgcg gtgccactca atcgtaattc
     3001 gcagtgcctg agcggtactc agggccattg tttcggcgct gcggctgacg agaacgctgt
     3061 ctcttccttt ggcagccagc gcgcggcgca gttcgtcgtt cgcgagcaat tcgtgcagcc
     3121 gctcggccag gtgctgcggg tcttccggcc ggtgcagcag gccgccgccg gtttcggcca
     3181 gcacttcggg gaatgccccg tggtcgggct ggacgactgg aactcccgcg gcgagcgctt
     3241 ccaagacaaa cagccccttg ggctcgcggt aggtcgtggg aaccgcgagc acatcgagcg
     3301 acgccagcag ctccaacttg ccgcggcgat cgacttcgcc gatatattcg aacgcggcgc
     3361 cgagccccgc ctcgcgcagt ctggcaaata caccctcggc ataagaccgg ttcttctcgc
     3421 ccatccagcc gccaatctgc aaacgggtgt tctccatccc cggcattcgc cgcagggcaa
     3481 tgaacgcctc cgccaggacg tgcagtccct tttccggggc gagccgagcc aggtaaccaa
     3541 tcttgggagg gccattcgcc tctgatcctt gaaccctgac ccctgatctc tcgctcacaa
     3601 agtccctggt atcaatcccc agcggcacga cctcgatccg ctcccgcggc aggctcagat
     3661 actccgccat cgcgtcggca tagaaccggg tgttggcgat gaagccgtcg atctgcccgg
     3721 cgaggcggcg aatctcggcc aaggcctgcg actggtgcgg ctgcgggagt ccccgcagga
     3781 agatgtcgtc gccttgaagg gtcacgacga cgggcacgtt catccgccgc ttgatctccg
     3841 gcacgcagcc cgcagtgagg atgttgctga acacaatcac ttgcgggtgc aaatcgcccg
     3901 cgagccactc ggccagccgc tcgacctctt tgcgctggaa tccggccgtg ccgcgaagga
     3961 tcgagagcgt caggtcggcg acttgctgtg gatcgatctt cacgctgcgg ccggaggccc
     4021 aattgatcag ccaaggctga tcaagccagc ggtcgagcca ttttggcagc cggcgaaaga
     4081 gcggcagctt ctgctgcagg tacatattga cgccgccgta gaagatgcgg cggccggaga
     4141 catcttcttc gtcggtacgg atcggcgtgt agagcggaac gagctgcacg tcgtggccct
     4201 gcgcgatcaa ggcgcgggcg agcgtgttgt cgtgcaggca gctgccgcag atcattcccg
     4261 ccgcgccggc cgtgagatag gcgatcttga gcggcggcgg gctggaagag ttggatgtca
     4321 tcgggaacca aggaaccgaa gaaccaaaca aacgaaaatc caaagaactt agctagtgca
     4381 ccatctgtct tggttcttcg gttcttcggt ctttcgagat ttcctgcaac cgcggcaatt
     4441 caaccctttg cgcgctgctt cccagagcat tgtacgtccg ccggacggcc agaaagccaa
     4501 gtgccagaca ggcagccatc ccgaaccagc gcgtcggtcc gctttcgaaa acaatggccc
     4561 cattgaaggc gataaacagc aggaaaacgt gaaccccaac ccgccaggct ggtgtctgcc
     4621 cccacggcca ggctgggcgc ggcacgctgg ttgcacaact gccggcagcg gccggccgac
     4681 tgccggtcca caaccagact acatccgcaa cccacgcgat aagaaacaag tagctgccgt
     4741 agatgccgtc gccaaaggca aaacccagca tccgccgggt ctcgtcggcg gtggtctgcc
     4801 acgcggcggc atgactccag ccgtgataga agtgaaacgc acacgccacg tgaacgacga
     4861 acagggcaca gccgccggtc cagagccagc gcgcggtccc cggccagcgc tcgctcttgg
     4921 ctgtcagcca tccggccagg tacgcgacgt aacaggccag agccagacgt atcgtccact
     4981 tggtgagcag cggacccatg atcgacactc gagcggcagg acagcggctg gcggcggcaa
     5041 gaatttcagg cggggctgcg tccggcgagg aagactcaag cggtcgcggc gaatacagca
     5101 tacacagacg tgcgcgccgc tgcacggagc ggctgcgaag acattcgcgt ccgctttggc
     5161 agaacccagc aggtcgcgac agcgtagtga gcagtggacc ggattggtcc aggtaatatc
     5221 gaacgaatcg agtatttccc aatatcaaac ctcggcaagg agaacagcac gcccagcatc
     5281 tcaagcgata aagtcttcgc ctcggcggac agaccaaagc ggagacggga ggaggcaacc
     5341 atgaaacgca acgaattcct gaaacaggtc atgcgcacgc tcgtcgcccg gcgcgacgcg
     5401 ctgcgaacga cgctcgcggg tgatgcggcg gtacttcgcg ccggccagga tgccggtgtc
     5461 ggcgacgaga tcgacgccgc cctggccagc gagcaggccg agatgcggtc gcagatggcc
     5521 agctacgagt cgcgggagct ggcccagatt gatgccgcac tggagcggat caaggcgggc
     5581 tcctacggcc gctgcgacaa ctgcggtgac ccgatcgcct cgctgcggct aaagaccttg
     5641 ccgtacgcca gcgagtgcat cggctgcgcc cgcggggcgg agcgccgcag tgcccgctcg
     5701 gtcaatcgca tcgcgggata cgacatcgtg cccggcccag cgcgcgatga aagtctggag
     5761 gagatcggct aattcggcag aaacggtctg caatgaaact cggggcggcg ctcgacgcag
     5821 gttgggcgcc gccttttgcg ttatattagg cggcaggtga gctcagtttg actctgagga
     5881 gtaccgccat ggtccgcagt ttcaccgctt cgctgctatt ggtcgcgctg ctcgcccagg
     5941 ccgtgcccgg ccaggaacca gccaagagca gccccacgcc ggaaatgatc aagaagctcc
     6001 aggacgatct gactaatgtg aagctcatcg gcagcttcac gatcgccggc aaggaagacc
     6061 agacgccgaa gcccgaggaa tacacgatct ctagtgcgct caagctgccc gacggggacg
     6121 tctggctgat caaggcccgc atcaaatacg gcgacaagga cgcgaccgtt ccgatcccgc
     6181 tggagatcaa gtgggccgga gatacgccgg tgatcacgct caccaacctc gagattcccg
     6241 gcctgggcac gttttcctcc cgcgtcgtca tttacgaagg ccgctatgcc ggcacgtggc
     6301 agcacggcaa agtcggcggc cacctgttcg gcaagttgga gaaggccacc gcgaacgatc
     6361 cagccaaaac cgagcccaaa ccggccgaca aatgagctgg cggcgatggc gctcgtgagc
     6421 tagagttgcc cgccagtctt tccaaatctc tagccccgcc gccatgttcg aactggtcga
     6481 ttttgtcctt caattcgacg agcacctcga caagctcgcg ggcgacttcg gcgtttggac
     6541 ctatgcgatc ctgttcgcaa tcgtcttctg cgagacgggc ctggtggtaa cgccgatcct
     6601 gccgggcgac tcgctgctgt tcgccgtggg ggcgttctgt gcccggccga accagccgtt
     6661 ggaaatctgg actgcgacca tcttgctcac gatcgcggcg atcctcggtg acacggtcaa
     6721 ttaccacatc ggcaaatacc ttgggcccaa ggtgctcagc ggcaagctct cgcgctggct
     6781 caaccccaag tacctggagc ggacgcagtg gttcttcgac cgctacggcg gcaagacgat
     6841 cgtgctggcc cgcttcgtgc cgatcgtgcg gaccttcgcc cccttcgtgg cgggcgtggg
     6901 cgcgatgcac tatggccgct tcctggccta caacatcttc ggcggtattg cctgggtcgt
     6961 gatctgcacg caggccggct tctggctcgg gaacatcccc gtcgtccgcg aacgattcga
     7021 gctggtcgtg ctgctgatcg ttgctatctc gctgttgccg gcgctgatcg agtactgcct
     7081 gcaccggttt gggcggaagc cggcggcgga aaaggcgctg tagtgaacag cctctgtggc
     7141 gttcccctgc ttcataaccc cttcgctgcc agatagcgct cggcgtcgag ggcggccatg
     7201 cagccggtgc cggcagacgt gatcgcctgg cgatagtagt cgtcggccac gtcaccggcg
     7261 gcatagacgc cctccttgct ggtgaaggtt cgcagcggcg ttgtgtattt gatgtagccc
     7321 atggcattca gctcgagctg cccgcggagg aacgccgtgt tcggcgtgtg gccgatcgcc
     7381 aggaacaggc cgctggccgc gaggtcgtgc gtgccccctt cccggttgtt gcggacccgg
     7441 atgccggtga cgccgctctt ctcgtcgccg agcacttcgt ccacttcgcg gaaatagtgg
     7501 atctggatct tgggattcgc ctgcgcccgg tcctgcatga tcttgctggc ccggaagacg
     7561 tcgcggcgca tcagcatgtg gaccgccgag gcgtacttcg tcaggtaggt cgcttcttct
     7621 actgccgagt cgccgccgcc gaccacgacc agcggcttgt tgcggaatcg cggcagcgcc
     7681 ccgtcgcaaa cggcacaggc gctgacgccg cgattcttgt acttctcctc cgaggccagt
     7741 ccgaggtaat tcgcccgcgc tccgctggca atgatgatcg tcagcgcctc gtgcgtcttg
     7801 ccttccagcg tggtcagttt gtacggctgc ttgctgagat cgacgctgac gacatcgtcg
     7861 gtgatgatcc gcgcgccgaa attcagtgcc tgctgccgca tcaggttcat cagctcgggg
     7921 ccggtgaccg catgcggatg ctcgtgcagc ggctccaggt acgtatcgac gcgatcctta
     7981 gcgacagcgc tgcggatgta ttcgtgcaag tggccggacg ggaaaccggg atagttctcg
     8041 acctccgtcg tgaggttgag ctgccccaaa ggcaacgtgc cggcgacccg gttgtcctct
     8101 gtctccgctc cctcgaagac gagcgggtgc aagttcgcgc gggaagcata gatcgcggcg
     8161 gaccagccgg cgggtccgct gccgatgatg acgacatgtt ctgacataaa ctccatccag
     8221 cgtgcaaagt tgaatcgccc gaattctagc agcgagcggc gggccgtaaa cgggcttcac
     8281 ggccggcgaa acagggctat ggcgttggcc gtcgcttgac tggccaggtc ggcgatggac
     8341 actccgcgga cttgggccag gcactgggcc gtatgcacga cgcgggccgg ttcgttcggc
     8401 cgcttgccgc gaaagggctc gggcgaaagg tagggcgaat cggtttctac cagcaatcgc
     8461 tcggcgggaa cggtggccgc aacggcccgc aggtcgtccg acttcttgta ggtcagcatg
     8521 ccggcaaagc tgatgtgcat tccaacgtcg agaaactccg ccgcctgctg ggccgtgccg
     8581 gtgaacgagt gcattacgcc ccgcagcggt ccgcgctcgc gggcctggcg gagcatggcc
     8641 agaatctccg ccgccgtctc gcggaggtga atgaccaggg ccagcccgct ctgctgcgac
     8701 aggcgaatgt ggcggtcgaa atagtcctgc tgcacggcca gcggcgtgtc cttccaataa
     8761 aggtccagcc ccgtctcgcc cagggcgacg acctccggct cgccgctcag ccgcacgatc
     8821 tcgtcccaat cgcccggctg cgcctcggac gcgtgattgg gatgaatgcc cgccgacgag
     8881 cgagcgccgg caaagcggcg ggccagctcc aggcactgcc ggctggactc ggcggtcgtg
     8941 ccgacggcca gaatccgcga aacgcccgct gcggtggctc gctccacgac gccggccaca
     9001 tcggccgcga tctgctcgtc ggaaaggtgg gcgtgcgtgt cgtacaggtc catggcggat
     9061 tgcttcgcta ttcgatccgc agcagcagat ccccggtctc gatgcggctg ccgggcttga
     9121 ccagcacgtc ggccacgcgc ccggcgacct cgctgctcac ggtcgtctcc atcttcatcg
     9181 cttcgagcgt gaagagcttt tggcccctgg tcaccttgtc gccggccttg accacgaccg
     9241 tgacgaccat gccgggcatc gccgcgccga cctgcttggg atcggccggg tcggccttga
     9301 tgcgtttatt gcccgcggcc tccagcgagt tatcgacgac gctcacgtcc cgcggctgac
     9361 cgttgagctc gaagaacacc gttcgcgtgc cgtcggcgtg tggctcgctg acggtgagga
     9421 acttgatgat cagcgtcttg ccctcttcga tctccacgct gatctcttcg ccgacctcct
     9481 ggccgtagaa aaagaccggc gtcggcagca cgctggtgtc ggagtacgct ttcgcgtggg
     9541 cggcgaactc ctgatagacc ttcggataga gcagatacga aagcgcgtcg cggcggttgg
     9601 gctcgcgggc aatcatcccg cggactctct ccaaggcggc gtcgaaatcg gccgccggca
     9661 gacttccccc ggcgcggccc ttcaccggct gccggtcctt gagaatccgc ttttgcaagc
     9721 ctttgggcca gccgccaggc ggctgcccca tcatgccgga caagaggtcg atgaccgact
     9781 cgggatacgc cagctcgcgg tctccttccg cgatgtcctt ggccgagagc ccgttggcca
     9841 ccatgaacag ggccaggtcg ccgacggcct tactgctggg cgtgaccttg acaatgtcgc
     9901 ccagcagttg attggcgtca gcataggccc ggcagacttc ctgccagcgg tcggccaggc
     9961 ccagggcccg cgcctgctga aacaggttcg tgtactgccc gccgggcatc tcgtggctgt
    10021 acagctcagc ggtcgccggc agcaggttgc tgtcgaacgg caggtagaac tcgcggactg
    10081 cctgccagta gtcggaaatc tcggccagct tgtggccatc caggcccgtg tcgcgcttag
    10141 tgaaccgcag ggcttcgacg accgcgttca ggtttggctg gctcgtgccg cccgacatgg
    10201 ctgacatggc cccgtcggcg atgtcgagct tctcttcggc cgcttccagg atcgaccccg
    10261 cctggatgcc cgccgtatcg tgcgtatgga agtggatcgg gatgtcgatc tcctgcttga
    10321 gcgtcttcac cagcaactgc gccgcgtacg gcttgcacaa tccggccatg tccttgatcg
    10381 ccaggatatg cgccccgagc ttctccaatt ctttggccag ctccacgtag tatttgaggt
    10441 cgtacttgct ccgttgggga ttgaggatgt cgcccgtgta gcagatcgcc ggttcgcaga
    10501 tcgcgccggt cttgagcacg gcctccatcg cgacccgcat gttcggcacc cagttcagcg
    10561 aatcgaagat gcggaagatg tccatccccg cctcggccga ctccttgcaa aactcgcgga
    10621 cgacgttgtc gggatagctg gcatagccga cggcgttgcc ggcgcggaac agcatctgga
    10681 agaggatgtt cggaattctc tcgcggagat cggtcaagcg ccgccacgga tcttccttga
    10741 gaaaccgcag ggccgtatcg aacgtcgcgc cgccccacat ctcgagcgaa aacaggccgc
    10801 tcgcattgcg ggcgtagaca tcagcgatgt tgagcaggtc cctggtgcgg aaccgcgtgg
    10861 ccaagagcga ctggtgcgcg tcgcggaagg tcgtgtccgt aagcagcagc ggcttctgct
    10921 tcaaaatcca ctcgccgaac ttcttcgggc cgagctcctg cagcttgtcg cgcgtgcccg
    10981 gcggcggcgg cgcgttcaaa tcgcaggccg gaacgggcgc ggcggcacgg cgcaccgcct
    11041 tgggcagccc cttcacgagc ggatggccgt tgaccacgat ttcgcccagg taggtcagca
    11101 gccgcgtggc ccggttccgc cgccgcggca gggcgaacaa gtcgggtgtc tcgtcgatga
    11161 accgcgtagt gacctgcccc tcgaggaagg tcgggttggt gaccagacgg atcaggaact
    11221 ggatgttcgt tttgacgccg cggacgcgga actcctgcag gcagcgctcc atgcgggcgc
    11281 aggcgtcgat gaaccgccgg ccgcgggccg tgactttcac cagcagcgaa tcgtagaacg
    11341 ggttgaccgt cgcgccgctg aacgccgtgc cggcgtcgag gcggatgccc atgccgctgg
    11401 ccgaacggta gtgggcgatc cggccatagt cgggcaggaa attgttggtc ggatcttcgg
    11461 tcgtcacgcg gcactgcacg gcgtaaccgg tcgtctgcac ggcgtcctgg ctgggcaggc
    11521 cgatctcggg atcggatagc ggcacgccct gggcgacgag gatttgcgac ttgaccaggt
    11581 cgatgccggt gacctcctcg gtcaccgtgt gctcgacctg aatccgcggg ttgacctcga
    11641 tgaagtaatg ctggttggtg tcgcgatcga cgaggaactc gaccgtgccg gcgttctcgt
    11701 atttgacggc ccggccgatc ttgatcgcgg cgtcgagcag cgcctgccgc acgctgggat
    11761 cgaggttcgg tgcgggggcg atctcgacga ccttctggtg tcgccgctgc accgaacagt
    11821 cgcgctcgaa gaggtgcacc aggtggccct gctggtcgcc gagaatctgc acctcgatgt
    11881 ggctggcgcg ggtgatgaac ttctcgagga agacgtccgg gctgccgaac gcggcgagcg
    11941 attcgcgctg agcttgctca aaggcagccg caaactcgct ctccgactgc acgacccgca
    12001 tcccgcggcc gccgccccct ttcgccgctt tcaggatcac gggatagccg agctttccgg
    12061 cgatcttgag cccttccttg gccgaggcga tcgcgctgga tccgcccagc acgggtacgc
    12121 cgacccggct ggcgatgctg cgggcaaaag tcttgtcgcc gagttgcgtc agggccgctt
    12181 cgtgcgggcc gacgaaggtg atgccggcct gctcgcaggc gcgagccagt tccgggttct
    12241 ccgacatgaa gccgtagccc ggatgaatgc cgtcgatgcc gtgctccttg gccacggcga
    12301 tgatgttggc gatgttcagg tacgagcgga tcggctcgcc cgcttttccg acgaggtagg
    12361 cttcgtccgc cttgaatcgg tgcagggcat agcggtcttc gtgcgaatag atcgctacgg
    12421 tgcggatgcc cagctcgtgg gcggcgcgaa aaacgcggat cgcaatctcg ctgcggttgg
    12481 cgaccagcag cttggtgatc ttgggcataa cggaccgcaa gcgggcgaaa caggatttcc
    12541 agggcggctg ccctggcgcg atccgcctga ccgccgggga gaggtgagtt tacactccga
    12601 gccccgccgc gcaacagggc ttacatcggc ggacgcggcg gcttgggagg catcggctgc
    12661 ggctcgccgg gcttagcgcg catcggcatc ggcgcttctc tgggcttggg cggcggcggg
    12721 acggcgagct cggccgtcac gatcgtctcg tcctcgccgc ggcggacaag cacctcgacg
    12781 cggtcgccgg cgaaatggcg gccgaggatc gaccgcaggt gcgtgacgtc cattatctcc
    12841 gcgccgccga ctttcaggat tttgtccccc ttttccaggc cggccttggc ggcgggagag
    12901 tcgggcacga cttctttgat ctctgcgccc gaaggcggct cgccgaaggg ctcgctggca
    12961 acgcccagga aggcatgctt gagcgtctcg ccggccttta gccggccgat gatctccgac
    13021 aacccttcga gcggaatggc gaagccgatg cccgagtcgt accattccgc gccggcggct
    13081 tcctcgcgcg agccgggcga cagcggcacg cagacgccaa tcacacggcc ctccagatcg
    13141 accagcggcc cgccgtagtt ggccgggctg gtgttggcgt cggtctgaat cgccttggtc
    13201 gagatgcggc tggtggccga gatgatccct gccgaaaggg ccggctcttg tgcgccgaag
    13261 cccacgccga tcgccaccgc ccattggccc accttcagct cgtcgcgcgg cgctagctgc
    13321 ggcaccggca agtcggcgag cccatcgact ttcagcaggc agattttgcg cgtctcgtcg
    13381 cggcccaaga gctgggccac gtgccgccgg ccgccctcca tgaccacggt gataatcggc
    13441 ggcttgcgca ggaaattgaa cgtgctggtg atgatgtagc cgtcgggcga gaggatgatc
    13501 cccgtcgtcg gcccctcgcc ggccgcctgg tatccgccgc cgcccactcc tggcgcgatg
    13561 ccgccaaacc cctcgatccg caccagcgac ggctgcacct tcgccgccgc tgcgcggaag
    13621 gccttcgggg cgagcagcgt gccgcgggtc gcggtttcct ggcctgcaag attcgcggcc
    13681 gccacgagaa gaatcaggac ggcggcgaat cgaggcccgg agggccgaca ggcaatcgcc
    13741 ccgaccgtca ggtcggggat cgtgtagacc ggatagaatc gaaggcccgg agggccggca
    13801 cattgcgggg tcatggttgg ctcaatctgc gccgaatcgg atgtgtcggt cttccggacc
    13861 ttcacgatcc gcttcgatag ctatccccga cctgacggtc ggggctgtta ccggccgggc
    13921 ctccggccct ggctcacttc tccgccaccg gcgtcagctt cacgcttacc aaatcgttct
    13981 tccgcttcac ttcaatcacc acctcttgtc caatcggcag ggcttcggcc agtcgcttgt
    14041 agtcactggc gtcgcggacg acctggccgg cgatcgtgac gaccaggtcg tcggtcttca
    14101 ggccggcccc c
//
LOCUS       DASRJB010000082        10475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_307574, whole genome shotgun sequence.
ACCESSION   DASRJB010000082 DASRJB010000000
VERSION     DASRJB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307574"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1406)
                     /locus_tag="VFB80_02340"
     CDS             complement(99..1406)
                     /locus_tag="VFB80_02340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN32620.1"
                     /translation="MSRRVVITGIGLVSPLGNSPERLWSALAAGQSGVRELKSIPVEA
                     FPTRFGAEALEFTGAIEDFGPLEKAKARNVKKNLKAMCREMQFGIAVAQLALADAKFP
                     LDSADLDRVGCVYGSDYMLTVPQEFTSGMRKSLDGQETFDFRNWADKGLPEVEPLWLL
                     KYLPNLPASHISIFNDLRGPNNSLTMREASSNLAIGEAYCTILRGHADAMLAGATGAR
                     VHPARTIHVVTQEEIAVEGDDPAKLSRPFDKGRRGSVMGEGAAAIMLEELAYAQARGA
                     KILGEVIGFGSSAVSDRRGVADYKAAFTNVLKQSLASARLGPDDVGHINAHGLSSRRC
                     DADEAQAIAAVFAGRKQPLGVVAAKSYFGNLGAASGLVELIGSLLAQQHGRLFRTLNY
                     ETPDPECPVHVVAADGSAPGDSFIKLSITPQGQASGVAIRRFS"
     gene            1582..2016
                     /locus_tag="VFB80_02345"
     CDS             1582..2016
                     /locus_tag="VFB80_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32621.1"
                     /translation="MSIGPMDGESSTQLLADQFLRRTLPKSAWTHQAHLRVGLWHVLH
                     FAPDEALARLRTGIRALNESHGTPNTDSGGYHETITRFYVCWIASFLSQAGSDRPIDD
                     LAHELVHAAGDSQLPLQYYSRERLFSVQARQTWVEPDLQPLP"
     gene            complement(2019..2444)
                     /locus_tag="VFB80_02350"
     CDS             complement(2019..2444)
                     /locus_tag="VFB80_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32622.1"
                     /translation="MRRDCQTTPCLGRASAIVWLSLFAAAGCGSQTLVPVSGKVTYKK
                     DGQPVTAGLVIFEPLNQKISARGEIQADGTFQLGTHGDNDGAMEGEYKVLIAPPPLPE
                     EGKRLRSPISSKYQNLDSTPLRFTVTRDRNKNKVNLEVE"
     gene            complement(2496..3455)
                     /locus_tag="VFB80_02355"
     CDS             complement(2496..3455)
                     /locus_tag="VFB80_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32623.1"
                     /translation="MWRNRSAFTLVELLVVIAIIGVLVALLLPAVQAAREASRRTKCA
                     NSLKQMGLAVHGFEDVHKVLVPARMENYGGVTWVVFILPFMEQGNFYNQWDINRWYYD
                     QGPSGNAIRQTQLPIFYCPTRRKPGGLSKNNDVPEIPFPGAPAGVNVPGALGDYATCN
                     GDTDADFIIAPNGVLIQAEVIYTSGQTNPTPSGQVLCSTPPCVVRSWKSRTRLANVTD
                     GTSNSFLIGEKHVRITSLGSNEDTALYNADRPDPTLRCAGPRFLLARTPNEAYNRQFG
                     SLHPGTCQFLFLDGSVRQLAVVTSGTVLGLLANRADGQAIPDF"
     gene            3922..5397
                     /locus_tag="VFB80_02360"
     CDS             3922..5397
                     /locus_tag="VFB80_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN32624.1"
                     /translation="MPRQNLFVLFVAAIACAVCYQRAARNRYAQAMTKAMNVVAENYI
                     DEVEPRVLFEGAMEGMVGQLDPYSAYTSPEEFAQFQQTMDGEFTGIGIMVDTDAATGR
                     LKVLDALIGKPAYVKGIRAGDILIEIDGKDTKNVPIREAVKYIRGKPGSRLSVVVQHA
                     GRPEPVTYELERTLIPLESILGDGRNEDGQWNFHLASHPEIGYVRIVNFGERTGEEFR
                     EALASLRQPAAIEGLIIDIRYNAGGLLTGATEVCDALLDEGLIVTTLGRHKVLMERYD
                     AAAGTDLPNSVPVVVLVDRLSASASEIVAACLQDHHRAAIAGQRTWGKGTVQNVVLLE
                     GGRSAIRLTVGSYHRPSGEKIHKWKGDKDSDPWGVRPDPGLEALVTSHENGLIVAARR
                     FRDLVPWNDLRAAASSANNTAPPAVTLPPMPPVPPQPGDAPPVPTPQPDDGQAAEQTP
                     PLEAEAAAKSKLDPAMIDPQLRTALEHLKRQTNKSQQPSAL"
     gene            complement(5825..6895)
                     /locus_tag="VFB80_02365"
     CDS             complement(5825..6895)
                     /locus_tag="VFB80_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32625.1"
                     /translation="MRTLLTFILLASPALGGTIDTVAGTGQAANNGDSGQALKTNVGQ
                     PFGVEIGPDGALYICEVQNHRVLRLDLKSGELKTVAGNGTKGYAGDGGPATKAQLNEP
                     YEVRFDRAGNMLFVEMQNQLVRKVDRQTGTISTVAGTGQKGFSGDGGQAGAATFSSPH
                     SIALDAQDNLFIADIGNHRIRRVDAKTGVVTTIAGNGERQQPADGQTAAGKPLVGPRA
                     LIVDGNTLWIALREGHAIWTMDLAAGTLRHIAGTGKPGYSGDGGSAKTATFNGPKGIA
                     LGPDKCLYVVDTENHAIRKFDPASGTINAVAGVFPAKKGGDGDGGPATAATLDRPHGI
                     CVAEGGTLYIGDTNNHRVRRVK"
     gene            complement(6935..8995)
                     /locus_tag="VFB80_02370"
     CDS             complement(6935..8995)
                     /locus_tag="VFB80_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN32626.1"
                     /translation="MLAYLVIREGTKWTDVYRLVPGRTVTIGRAGTNQIVIKDERTSR
                     NHAEIFVTRGAWTLRDLDSRNGTFVGERQVRGDYALQAGDVVRIAQCHLAFVQDLTQA
                     FPDPITGSPGLAQLPIGEETVSSALASETGEIEDPASVLDEAHEPTTITHRRNQTRFL
                     VPGGQNDVAVPRLGQAAAKLCRLAFELASQPDATSVAHLALNGLFDSTHVDAGALLLA
                     RDADSEPTPADLQVVASRTDIEPSYHRASGFLASTVLRDGEAVLARNIEGDSNLATKD
                     SKGEFHATGAICAPIRQDRKVIGLIHLYSTSTERVPDPEDLEFTLAVADNVALALKNI
                     ARQQELAENLNQTRSEILELRKQLGAESELVGSSPVLANVQQQIARAAPSRATVLIRG
                     ESGVGKELVARAMHYASPRKKGPFVCLNCAALTETLLESELFGHERGAFTGATERKIG
                     KFEQAHQGTLMLDEIGEMSPQIQAKFLRVLEGHPFERVGGSQPIKVDVRTIAATNRDL
                     EKAVAEGNFRRDLYFRLRVVEMFVPPLRKRPEDIIELANYFLDRYIAETGRKIRGFTP
                     EALHVMQKYRWPGNVRELKNVIERAVVLARADFIEPDDLNLSSIATVGESGDVASPKS
                     TFEPMSLDEIERRHIHATLRATGWNKSRCAAILGVERSTLDRKIRRYDLKPPHARADE
                     WQAE"
     gene            9311..>10475
                     /locus_tag="VFB80_02375"
     CDS             9311..>10475
                     /locus_tag="VFB80_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HZN32627.1"
                     /translation="MSISVGRKSAVRWSVFCLAAIAGLLLAADVGFAQCGFGRFPSVG
                     GVHIDANGALKSPDPADVNALAVAMAKAAKPVAGELNEKVALRKVSLRAIEAELAKAG
                     QPNAAELPEEIRFLAGIQRIQYIFVDPDHHDIVLAGPGEGWKLDGRGNYVGVTTGLPV
                     LRLEDLIVALRNVENARRGGITVSIDPTEEGRKKFEQFMKTQTRYTPAVLEGMATALG
                     NQTITITGVPDTSRFARMLAASDYKMKRIAMELEASPIRELPSFLSMLQKGGAKLGNL
                     MPRFWMACNYEPMGRSEDGLAWELRGKGVKVMTEDDYVAGGKATGTGKANPLAQKWSD
                     LMTEHYDKLAVKEPVFGDLRNLMDLCVVAALIHKEGLLERAILQIPTMQDKDSK"
BASE COUNT         1816 a         3283 c         3462 g         1914 t
ORIGIN      
        1 ttgacaggcg gcccggcgtc gctcgtttaa cccgtagtcg gaggcgcagg cgctttcccg
       61 gagccgccgt cgcctccggc tccgggttaa acgaagagtc aagagaaccg ccggatcgca
      121 acgccgctgg cctggccctg gggcgtgatg ctgagcttga taaagctgtc gccgggagct
      181 gagccgtccg cggcaacgac gtggacgggg cactcgggat cgggcgtttc gtaattcagc
      241 gtgcggaaca gccggccgtg ttgctgggcc aggaggctgc cgatcagctc gaccaggccg
      301 ctggctgcgc cgaggtttcc gaaatagctc ttggccgcaa cgacgcccag cggctgcttg
      361 cggccggcga ataccgcggc gatggcctgg gcctcgtcgg cgtcgcagcg gcgcgagctc
      421 aggccgtgag cgttgatatg cccgacgtcg tcaggcccga gccgcgcgct ggcgagcgat
      481 tgcttcagga cgttcgtgaa ggccgccttg tagtcggcta cgccgcgccg gtcgctgacc
      541 gcggacgagc cgaagccgat gacttcgccg aggatttttg ccccgcgagc ctgggcgtag
      601 gcgagttcct cgagcatgat cgccgcggcc ccttcgccca tcaccgaacc gcggcggccc
      661 ttgtcgaacg gccggctgag tttggccggg tcgtctccct cgacagcgat ttcctcctgc
      721 gtgacgacgt ggatcgtgcg ggccggatgc acgcgagcgc cggtcgcgcc ggcgagcatc
      781 gcgtcggcgt gtccgcgaag gatcgtgcag taggcctcgc cgatcgccag gttgctcgag
      841 gcctcgcgca tcgtcagcga attgttcggc ccgcgcaggt cgttgaagat gctgatgtgg
      901 ctggccggca ggttgggcag gtatttgagc agccagagcg gctcgacttc ggggaggccc
      961 ttgtcggccc agttgcgaaa gtcgaacgtt tcctgaccat cgagcgattt gcgcatgccg
     1021 ctggtgaatt cctgcggcac ggtgagcatg tagtccgaac catagacgca accgacgcgg
     1081 tcgagatcgg ccgagtcgag cgggaatttc gcgtcggcca gggcgagctg ggccacggcg
     1141 atgccgaact gcatctcgcg gcacatcgcc ttgaggttct ttttgacgtt gcgggccttg
     1201 gccttttcga gcgggccgaa atcctcaatc gcgcccgtga attcgagcgc ctcggcgccg
     1261 aagcgggtgg gaaaagcctc caccgggatc gacttcagct cgcggacgcc gctttggccc
     1321 gcggcaaggg ccgaccagag ccgctcgggc gaattgccca gcggcgagac gaggccgatc
     1381 cctgtaatga cgacgcggcg tgacacgcaa gctgccaaaa accggaatgc cacggagggc
     1441 actccctacg gcgttccgga agctgggaaa tccgggccaa gcctgctggc ggtcccgatc
     1501 ccaggaccgg cattctattt aggtggcgaa gttccgtcga gtcaccctga atgcagggcc
     1561 tgaaccctgg tcgtgaagaa gatgtccatc ggacccatgg acggcgaatc cagcacgcag
     1621 ctcctggcgg accagtttct ccgccgcact ctgccgaaat cggcttggac acatcaggcg
     1681 cacctccgcg tcggcctctg gcacgtgctg cacttcgctc ccgacgaagc gctggcccgg
     1741 ctgcgaacgg gaatccgcgc gctgaacgaa tcgcacggca cgcctaacac cgacagcggc
     1801 ggctatcacg agacgatcac gcggttctac gtctgctgga tcgcgagttt tctgagtcag
     1861 gctggtagtg atcggcctat cgacgacctg gcgcacgaac tggtccacgc agccggcgac
     1921 agccagttgc cgctgcagta ttacagccgc gagcggctgt tctcagtcca ggcccgccaa
     1981 acctgggtcg agccggactt gcagccgctg ccctgaattc attcgacctc aagattgacc
     2041 ttgttcttgt ttcggtctcg agtcaccgta aagcgaagag gagtggagtc gaggttctga
     2101 tacttggaac tgatcggaga gcggagtcgc ttgccctcct ccggcagggg cggcggggcg
     2161 atcagcactt tatattcgcc ttccatagcg ccgtcgttgt cgccgtgcgt gcccagttga
     2221 aaggtgccgt cagcctgaat ttctccccgc gcgctaatct tctggttcag gggctcgaag
     2281 atcaccaggc cggcggtgac cggctggccg tccttcttgt atgtaacttt cccgctcacc
     2341 ggaaccagcg tttgactgcc gcagcccgcg gcggcaaata agctcaacca aacaatcgcg
     2401 ctggcccgcc ccaggcacgg agtggtttgg caatcgcggc gcatcgtgga cctcccagca
     2461 aatgaaatcg gcccgccgta aataagcggc cgcgattaga agtcgggaat ggcctggccg
     2521 tcggcgcgat tggccagcaa tcccaggact gtcccgctgg ttacgacagc cagttgccgc
     2581 accgagccgt cgaggaacag gaactggcag gtccccggat ggaggctgcc gaattgccga
     2641 ttgtaggcct cgttcggagt tcgcgccaga agaaagcgcg ggccggcgca gcggagcgtg
     2701 ggatcggggc gatcggcatt gtagagcgca gtgtcttcat tgcttccgag cgaggtgatc
     2761 cgcacgtgtt tttccccgat caggaaggaa ttgctggtgc cgtcggtgac gttcgccagc
     2821 ctggttctcg atttccagct ccgcaccacg cacggcggcg tcgagcagag cacctgcccg
     2881 ctgggtgtgg gattggtttg cccggatgtg tagatgactt ccgcctgaat cagcactccg
     2941 ttgggcgcga tgataaagtc ggcgtcggtg tcgccgttgc aggtggcgta atctcccaaa
     3001 gcgccgggca cattcacgcc ggcgggcgct ccgggaaacg gaatctccgg cacgtcgttg
     3061 ttcttgctca ggccgccggg cttccggcga gtcgggcaat agaagatggg gagctgcgtt
     3121 tggcgaatcg cattgccgct gggcccctgg tcgtaatacc agcgattgat gtcccactgg
     3181 ttgtagaaat tgccctgctc catgaacggc aggatgaaga ccacccaggt cactccgccg
     3241 taattctcca tgcgggccgg gacgaggacc ttgtgaacgt cttcgaaccc gtgcaccgcc
     3301 agccccatct gcttcaggct gttggcgcac ttcgttcgcc gcgaggcttc gcgggcagcc
     3361 tggaccgccg gcaacagcag cgcgacgagc actccaatga tcgcaatgac gacgagcagc
     3421 tcgacgagcg taaaggcgct ccgattccgc cacataacgc acccccccag cgcagaatgt
     3481 gatcggcccc cagcgacaac catccggttc ttctcgccct ccagcaccct aaagcattcg
     3541 caagttggga gtcaaccttg tccgcttcag atgtaatttg cgagacgtcc ggactggata
     3601 acgcgggcct gcgatgaagc acttgacata tagtggacgg ataaatgaga atccgctcca
     3661 caaccgcgcc cgcagatggc acgccgcctg gtagccgtcg ttccgtagca aggaatgaac
     3721 ttgcatcaac tgcatacgtg cataaaaacg caagcgacgt aagttatttc cagaacgtca
     3781 cttgcaccgc taaatgcagc ctgcaaaatc gcgcgattat gcagaatatg gatgctcgcc
     3841 ttcagcgtgt tgcccgccgg cggacggatt cgccacaatg acctttggtt cttcggttct
     3901 ttggttcttg gattttgccc gatgcctcgc caaaacttgt tcgtgctgtt cgtcgcggcg
     3961 atcgcctgtg cggtctgtta ccagcgggcg gcgcggaacc gctatgccca ggcgatgacc
     4021 aaggccatga acgtggtcgc ggaaaattac atcgacgagg tggagccgcg ggtgctgttt
     4081 gaaggggcga tggaagggat ggtgggccag ctcgatccct attcggctta caccagcccc
     4141 gaggagttcg cccagttcca gcagacgatg gacggcgagt tcaccggcat cggcatcatg
     4201 gtcgataccg acgccgccac cggccggctg aaggtgctcg acgccctgat cggcaaaccg
     4261 gcctatgtga agggcatccg ggccggcgac atcctgatcg agattgacgg gaaggatacg
     4321 aagaacgtcc cgatccgcga ggcggtcaag tacatccgcg gcaagccggg gtcgcggctc
     4381 tccgtcgtcg tgcagcacgc cgggcggccg gagcccgtga cgtacgagct ggagcggacg
     4441 ctgattccgc tggagtccat cctgggggac ggccggaatg aggacggcca gtggaatttc
     4501 catctcgcct cccatcccga gatcggctac gtccgcatcg tcaatttcgg cgagcggacc
     4561 ggcgaggaat ttcgcgaggc cttggccagc ctgcgccagc ccgccgcgat cgaaggcctg
     4621 atcatcgaca tccgctacaa cgccggcggc ctgttgaccg gggcgactga agtctgcgac
     4681 gcgctgctcg acgaggggtt gatcgtcacg acgctcggcc gccacaaggt gctcatggag
     4741 cgctacgatg cggcggccgg aacggacctg cccaatagcg ttccggtcgt ggtgctcgtc
     4801 gatcgcttga gcgccagcgc cagcgagatc gtggcggcct gcctccagga tcatcaccgg
     4861 gcggcgatcg ccggccagcg gacgtggggc aaaggcaccg tgcagaatgt ggtcctgctc
     4921 gaagggggcc gcagcgcgat ccggctgacc gtcggcagct accaccgccc cagcggcgag
     4981 aagattcaca aatggaaggg cgacaaagat agcgatccct ggggcgtgcg gcccgatcca
     5041 ggtctggaag cgctggtgac cagccacgaa aacggcctga ttgtcgccgc ccggcgtttt
     5101 cgggatctgg tgccctggaa cgacttgcgg gctgccgcta gctccgccaa caacactgcg
     5161 ccgcccgcag ttacgctgcc tccaatgccg ccggtgccgc ctcagccggg cgatgcgccg
     5221 ccggtcccca cgccgcagcc ggacgacggc caggcggccg agcaaacgcc gcctttggaa
     5281 gccgaagccg ccgcgaagtc gaagctcgat cccgcgatga tcgacccgca gctgcgcacg
     5341 gcgctggagc atttgaaacg ccagaccaac aagtcgcagc agccatcggc gctgtgatcc
     5401 gcgaccattg ggaggcgaaa gaggctttca gaaaatctgg attctggcgg gaataaatgg
     5461 ccgggttggc gcatctatac aatagtggca atgcgccgtc gaatacgatg gtgcggtgcg
     5521 ggtctttggc aagtcggtcg tcgaggattg caagggagta atgtacgcct gtaaatataa
     5581 tggcgcgagg gtaaggataa ttccgcgggg ggttcactaa cgcttctaac gtgagggcga
     5641 ttgctggcag tgattcgcgg cgcaagtcgg ttggcagcag cgagatgtga cgcggccgcc
     5701 agacgaatgg gccgtgaaaa tctcgacccc aatgggaatt atccttacct cgccgcgcca
     5761 actacttgcg gcgtcttgtg cggcattggg tcaggagcca ggggccagtg gtggcgggcc
     5821 tgggtcactt cacgcggcgg acgcggtggt tgttggtgtc gccgatgtag agcgtgccgc
     5881 cctcggcgac gcagatgccg tgcgggcggt ccagcgtggc ggccgttgcc gggccgccgt
     5941 cgccgtcgcc gccttttttg gcgggaaaaa cgccggcgac cgcattgatc gtgccgctgg
     6001 ccggatcgaa cttgcgaatc gcatgattct cggtatccac gacatagagg cacttgtccg
     6061 gtccaagggc gattcccttg gggccattga acgtcgcggt cttggccgag ccgccatcgc
     6121 cgctatagcc gggcttgccc gtgccggcga tgtgccgcag cgtgcccgcg gccagatcca
     6181 tcgtccagat cgcgtgccct tcgcggagcg cgatccagag cgtattgccg tccacgatca
     6241 gcgcccgcgg cccgaccagc ggcttgccgg cggccgtctg tccgtcggcc ggctgctgcc
     6301 gctcgccgtt gccggcgatc gtggtgacga cgcccgtctt ggcatcgaca cggcgaatgc
     6361 ggtggttgcc gatgtcggcg atgaagagat tgtcctgggc atccagggcg atgctgtgcg
     6421 gcgagctgaa cgtggccgcg ccagcctgac cgccgtcgcc gctgaagccc ttctggccgg
     6481 tgccggcgac ggtgctgatc gtgcctgttt ggcgatcgac cttgcggacg agctgattct
     6541 gcatttcgac aaacagcatg ttgcccgctc tatcgaatcg cacttcgtac ggctcgttga
     6601 gctgcgcctt cgtcgccggc ccaccgtcgc ccgcatagcc cttcgtgccg tttcccgcga
     6661 ccgttttcag ctcgccgctc ttgagatcca ggcgcagtac gcgatggttc tgcacttcgc
     6721 agatgtaaag cgcgccgtcc gggccgatct cgacgccgaa cggctgcccg acgttggtct
     6781 tcagcgcctg cccgctgtcg ccgttattgg ccgcctgccc ggtgcctgcc accgtgtcga
     6841 tcgtgccgcc cagggccggc gatgccagga ggatgaacgt cagcaacgtg cgcatcggaa
     6901 tctccttgtg cgatggcctc actggcgagc gcgctcattc cgcctgccat tcgtcggcgc
     6961 gggcgtgagg cggcttcagg tcgtagcggc ggatcttgcg gtcgagcgtg gagcgttcga
     7021 cgcccaggat cgcagcgcag cggctcttgt tccagccggt ggcccgcagc gtggcgtgaa
     7081 tgtgccgccg ctcgatctcg tcgagcgaca tcggctcgaa cgtcgatttc ggcgaagcca
     7141 cgtcgcccga ttcgccgacg gtggcgatcg acgacagatt caaatcgtcc ggctcgatga
     7201 aatcggcccg ggccagcacg acggcccgct cgatcacatt cttcagctcg cggacgttgc
     7261 cgggccagcg gtatttctgc atcacgtgca aggcctcggg cgtaaagccg cggatcttgc
     7321 gtccggtctc ggcgatgtag cggtccagga aatagttggc cagctcgatg atgtcctcgg
     7381 gccgcttgcg aaggggcggc acgaacatct cgacaacgcg gaggcggaaa tagagatcgc
     7441 ggcgaaaatt gccctcggcc accgcctttt ccaggtcgcg gttcgtggcg gcgatcgtcc
     7501 gcacatcgac cttgatcggc tggctgccgc cgacccgctc gaacggatgc ccctccagca
     7561 cgcggaggaa cttggcctga atttgcggcg acatttcgcc gatttcgtca agcatcagcg
     7621 tgccctggtg ggcctgctcg aatttgccga tcttccgctc ggtcgcgccc gtgaatgcgc
     7681 cccgctcgtg gccaaacagc tcgctctcca gcagcgtttc ggtgagcgcc gcgcagttga
     7741 ggcagacgaa cgggcccttc ttccgcgggc tggcatagtg catggcccgc gccaccagtt
     7801 ccttgccgac gccgctttcg ccgcggatca gcacggtcgc tcggctgggc gcggcgcggg
     7861 cgatttgctg ctgcacgttg gccaggaccg ggctgctgcc gaccagctcg ctctccgcgc
     7921 caagctgttt gcgcagctcc aggatttcgc tccgcgtctg gttgaggttt tcggccagtt
     7981 cctgctgccg ggcaatgttc ttcagggcca gcgccacgtt gtcagccacg gccagcgtga
     8041 actccaaatc ctccggatcg ggcacgcgct cggtgctggt cgaatagagg tggatcaggc
     8101 cgatgacttt gcggtcctgg cggatcgggg cgcagatcgc gccggtcgcg tggaattcgc
     8161 ccttgctgtc cttcgtcgcc aggttgctgt cgccttcgat gttgcgggcc agcaccgcct
     8221 cgccgtcgcg gagcaccgtc gaagccagga agcccgaagc ccggtggtag gagggctcga
     8281 tgtcggtccg cgacgcgaca acctgaagat ccgcgggggt cggctcgctg tcggcgtcgc
     8341 gggccaagag cagcgctccc gcatcgacat gcgtgctgtc gaacagaccg ttcagcgcca
     8401 agtgggccac cgaggtcgca tcgggctggc tcgccagttc gaacgccagc cggcagagtt
     8461 ttgccgcagc ctggcccagc cgcggaacgg cgacgtcgtt ctgccctccc ggaacgagga
     8521 accgggtctg gttgcggcgg tgcgtgatcg tcgtcggctc gtgcgcctcg tcgagcacgc
     8581 tcgccgggtc ttcaatctcg cccgtctccg aagccagcgc gctggagacc gtctcttcgc
     8641 cgatcggcaa ctgggccagg cccggcgagc cggtgatcgg atcgggaaag gcctgcgtca
     8701 gatcctgcac gaacgccagg tggcactgtg cgatgcggac gacgtcgccc gcctgcagcg
     8761 cgtagtcgcc gcgcacctgc cgctcgccca cgaacgtgcc gttgcggcta tcgaggtcac
     8821 gcagcgtcca cgcgccgcgg gtgacgaaaa tctcggcgtg attgcggctg gtccgctcgt
     8881 ccttgataac gatctggtta gtgccggccc ggccgatcgt gacggtccgc cccggcacaa
     8941 gacgatacac gtcggtccat ttcgtacctt cgcggatgac gagataggcc agcatcggcg
     9001 cggcggagag aaggatcgaa cactttgctg cgcgaaaatg cggaggggga tttaccccta
     9061 tgcaaaatcg ctcacgccgg ccgccctcag ggccgacccg cggctgcccc agccaccaac
     9121 ttaccttgga acgcaaactg caagcaatag ccgccgtttg ttgcaaagta ttgcccccgc
     9181 agttattctg aagaccgata cgattctcgt caggggcgac agttcgggca gactcgcttg
     9241 ccgcgcgcgg aaccagaaga ggccgccagc cggctgggtg cggttttgcc caagatcgag
     9301 gagttgcgtc atgtcgattt ccgttgggcg gaaaagtgca gtccgctgga gcgttttctg
     9361 cctcgccgcg attgcgggcc tgctgctggc tgccgacgtg ggctttgccc aatgcggctt
     9421 tggccggttc ccgtcggtgg gcggcgtcca tattgacgcc aacggcgccc tgaagagtcc
     9481 ggacccggcc gacgtgaatg ctttggccgt cgcgatggcg aaagcagcta agccggtcgc
     9541 cggcgaactc aatgaaaagg tcgccctgcg aaaggtttcg ctgcgggcga ttgaggcgga
     9601 gctggccaag gcaggtcagc cgaacgccgc ggagctgcct gaggagatca ggttcctggc
     9661 cggcattcag cgaattcaat acattttcgt ggatcccgac catcacgata tcgtgctggc
     9721 cggccccggc gagggctgga agctcgacgg ccgcggcaat tacgtcggcg tgacgaccgg
     9781 cctgcccgtt ctgcggctgg aagacctgat cgtagctttg cgaaatgtcg agaacgcgcg
     9841 ccgcggcggg attacggtct cgatcgatcc gacggaggaa ggccgcaaga agttcgagca
     9901 gttcatgaag acgcagactc gctacacgcc cgcggtgctc gagggcatgg cgacggcgct
     9961 cggcaatcag acaatcacga tcacgggcgt gccggacact agccgatttg cccggatgct
    10021 ggccgcgagt gactacaaga tgaaacggat cgccatggag ctggaggcgt cgcccatccg
    10081 cgagctgccg agctttctgt cgatgttgca gaaaggcggc gcgaagcttg gcaacctgat
    10141 gccgcggttt tggatggcct gtaactacga gccgatgggt cgcagcgagg acggcttggc
    10201 atgggagctg cgcggcaagg gcgtcaaggt gatgactgag gacgattacg ttgctggcgg
    10261 caaggccacc ggcacgggca aggccaaccc gctcgcgcaa aagtggtctg acctgatgac
    10321 cgagcactac gataagctag ctgtgaagga gccggtgttc ggcgatttgc ggaacctgat
    10381 ggacctgtgc gtcgttgcgg cgttgatcca caaggaaggc ctgctggagc gggccatcct
    10441 gcagattccg acgatgcagg acaaagacag caaat
//
LOCUS       DASRJB010000083         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3410332, whole genome shotgun sequence.
ACCESSION   DASRJB010000083 DASRJB010000000
VERSION     DASRJB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410332"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..753
                     /locus_tag="VFB80_02380"
     CDS             418..753
                     /locus_tag="VFB80_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32628.1"
                     /translation="MTCQELADRVQRLQPEASARDVARVCLLLSNSVDDVSKLDDDDL
                     LTAAWREAGLRLEAATDQHAAMTEELESLARSDPRQFTPDQIWILIRAIKVQGQILQL
                     YVGEPVLDA"
     gene            886..2028
                     /locus_tag="VFB80_02385"
     CDS             886..2028
                     /locus_tag="VFB80_02385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32629.1"
                     /translation="MLTTTRHDVYPTITHGGRPVPTEPNELVAQYQEILTERRLNWTN
                     HLQLIKLLGEGGQGVVYQTELRGTDDFTLPAALKIFSPERFENVRAYDEAMQRVARAS
                     ARIAQIQHDNLLYVYNFVDRHRVRMMVMELIDGYDLARLLSMNMLDRVRDRVSNRRWE
                     YINQVIVTAGPNQPRMKAGVAVAIVRDCLNALAALHRHGIVHGDIKPSNIMLKRTGIA
                     KIIDIGSAFELRDPPHARNCTLAYAAPEVLENRETTPRSDLCSLGYVLIELLAGRQVF
                     SSSNDLRELLEAKRSTAQRLHEILPEEVLRCELLCAFIRGMIAADPIRRFPSAEAAEL
                     LKEGAAAFHRQLVMGNLASEYDNDIRLWLEELKELDAQDDIASSAA"
     gene            2060..2413
                     /locus_tag="VFB80_02390"
     CDS             2060..2413
                     /locus_tag="VFB80_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32630.1"
                     /translation="MPAVVFLSSDLMFSSRVAGAAGTLDVPLQLVAQPANLAAKLTAD
                     CRLVLVDLAIAGLDLPVAIATVRGAAPQARIIAFGAHVDEAALAAAQSAGCDLVLSRG
                     QFHKQYVELLKSVGE"
     gene            complement(2422..3135)
                     /locus_tag="VFB80_02395"
     CDS             complement(2422..3135)
                     /locus_tag="VFB80_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN32631.1"
                     /translation="MSYLVQPGEKLVWWGQPSRILYAKYALLMLGLNSVVYVGLLSFV
                     ASRADPQSVSLSAVTIGVWALSLLVSSGPIVGTLAWHWSCAGRTLYLLTTERAIRYEP
                     RWMSGYKIVEARPEDLEGGLVFDALWNNKQVRRLKEETLAPKLAERLKHRDGRIRRQA
                     IVTAAALAKQNRIPFSVLVDALWSEDRFIRKQAAVGLGKLRKDGRLAVRDLRRLVFDE
                     DRAVSQAALHSLEAITRAA"
     gene            complement(3187..4518)
                     /locus_tag="VFB80_02400"
     CDS             complement(3187..4518)
                     /locus_tag="VFB80_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN32632.1"
                     /translation="MSKTISRRTVLRGLGCALGLPLLEAMRPARGFAADAANRPPVRL
                     AFISFPNGAIMDAWRPKGEGESFEFGPTMQPLADVKSQITVFSGLAQENGFAKGDGAG
                     DHARSSASLLTGAHPVKTSGANIRVGQSVDQAAAERIGHLTRLPSLEIGIERGRDAGS
                     CDSGYSCAYSNNISWKNATTPMAKEINPRLVFERLFGNSLSGGQTRRSRVRQSVLDVV
                     SEQAADLKNRLGQTDRQKIDQYFASVREIELRITRAATAPPAEPPKDFAVPTGLPGEV
                     QEHIRLMYDLMVLAFQTDTTRVITFMTANEGSNRPYRMVGVNNGHHELSHHRSEQDKV
                     DQLKKIDLFLVTEFARFLKTLQATPDGGGTLLDNCLVLYGSGLSDGNRHRHDDLPIIL
                     AGKGGGTIPAGRHIHVGEAPLNNLFLSLLDRMDAGVPSLGDSTGRLKAIDG"
     gene            complement(4515..>4747)
                     /locus_tag="VFB80_02405"
     CDS             complement(4515..>4747)
                     /locus_tag="VFB80_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HZN32633.1"
                     /translation="LELARVLDKRRGEFARCLAEKMLTFGLGRELSPPDRCAVDKIVD
                     EVESRDFRFSALVSAVVDSDPFRKRRGEGAAP"
BASE COUNT          839 a         1586 c         1483 g          839 t
ORIGIN      
        1 cggataacac ggatcggaaa tgatttgtct gatccgtgtt atccgcgtcc atccgcggcc
       61 attcttacac aacccagctc gagtcccaac cccggcggtc gcctagggct gcgccagagt
      121 agcggcaatt cccccggatt ccgattctga acgcagtggt gacatgccct tgcggcccct
      181 tgcggcttcg gccccaagtt cgaaattgca ttttggcatc ttcgtcgcca aaacgccaca
      241 agttcggccg gcaaaaacgt caagccagaa ttccgggcgg gtttgtggac ttaacggatt
      301 tttcagtcct tctgcaacac acgctcacga ttttgtcgct cgttttgcaa atgcgagcta
      361 aagtttttgt agcggtggcc gcgggtgccg ccacgagtag tggagcttgg ggaacgtatg
      421 acttgtcagg aacttgccga tcgtgtgcag cggcttcagc ccgaggcttc cgcgcgcgac
      481 gtggcccgcg tctgtctgct cttgagcaac tccgtcgatg acgtcagcaa gctggacgac
      541 gacgacttgc tgacggccgc ttggcgcgag gccggcctgc ggctggaagc cgccaccgac
      601 cagcacgccg cgatgaccga agaattggag tcgctggccc gcagcgaccc gcggcaattc
      661 acgcccgatc agatctggat cctgattcgc gccatcaagg tgcaggggca aatcctgcag
      721 ctctacgtgg gcgagcccgt gctggatgcc taggcgagcc gtggtcggcg ggtaaaacct
      781 ctttacgccc caaaggcgcg gggcgtacat tgatgacggt ttcgtaaggc cgcgcgacgg
      841 gccgctcgcg ccggcaggat tcgcactcgt tcatggagcg gacgcatgct cacgacaacg
      901 cggcacgacg tctatcccac catcacgcac ggcggccggc cggtcccgac agagcccaac
      961 gagctcgtcg cccagtacca ggaaatcctc accgagcggc ggctgaactg gaccaaccac
     1021 ctccagctca tcaagctgct cggcgaagga gggcagggcg tcgtctacca gaccgagctg
     1081 cggggcacgg acgatttcac gctgccggcg gccctgaaga tcttctcgcc cgagcggttc
     1141 gagaacgtgc gggcctacga cgaggcgatg cagcgcgtgg cccgggcctc ggcccgcatc
     1201 gcccagatcc agcacgacaa cctgctctac gtttacaact tcgtcgatcg ccaccgcgtg
     1261 cggatgatgg tgatggagtt gatcgacggc tacgacctgg cccggctgct aagcatgaac
     1321 atgctggatc gtgtccgcga ccgcgtcagc aaccgccgct gggagtacat caaccaggtc
     1381 atcgtgacgg cggggccgaa tcagccgcgg atgaaggccg gcgtggcggt ggcgatcgtc
     1441 cgcgactgcc tgaacgccct ggcggccttg caccggcacg gcatcgtgca tggcgacatc
     1501 aagccgtcga acatcatgct caagcggacg ggcatcgcca agatcatcga catcgggtcg
     1561 gcgttcgagc tccgcgatcc gccgcacgcc cgcaactgca cgctggccta tgccgcgccg
     1621 gaggtgctgg agaaccgcga gacgacgccc cgcagcgacc tctgctcgct gggctacgtg
     1681 ctgatcgagc tgctcgccgg gcggcaggtg ttttccagtt ccaacgacct ccgcgagctg
     1741 ctggaggcca aacgctcgac ggcccagcgg ctgcacgaaa tcctgcccga ggaggtcctc
     1801 cgctgcgagc tgctgtgcgc gttcatccgc ggcatgatcg ccgccgaccc gatccgccgc
     1861 tttcccagcg ccgaggccgc cgagctgctc aaggagggcg ctgccgcctt ccaccggcag
     1921 ctcgtgatgg gcaacctggc cagcgaatac gacaacgaca tccgcctctg gctggaagag
     1981 ctgaaagagc tcgacgccca ggacgacatc gccagcagcg ccgcctgacc gtggggcaga
     2041 cattcctgtc tgccacaaaa tgcccgccgt ggtcttcctc tcctccgacc tgatgttctc
     2101 cagccgcgtc gccggcgcgg ccggcacgct cgacgtgccg ctgcagctcg tcgctcagcc
     2161 cgccaatctt gctgcgaagc tgaccgccga ctgccggctc gtcctggtcg atctggccat
     2221 cgccgggctc gacctgcccg tcgcgatcgc caccgtccgc ggggctgcgc cgcaggcccg
     2281 gatcatcgcc ttcggcgctc acgtcgatga agccgccctc gccgccgctc aatccgccgg
     2341 ctgcgaccta gtcctcagcc gcgggcagtt ccacaagcag tatgtggagt tgctgaaaag
     2401 cgtcggggag tgacctagcg gtcacgcggc cctcgtgatc gcttccaacg agtgcagggc
     2461 ggcctgagag acggctcgat cttcgtcgaa gaccagcctg cgtaggtcgc gcacagccag
     2521 tcgaccatcc tttctcagct ttcccagtcc aacggctgcc tgcttgcgaa tgaaacgatc
     2581 ctcgctccac agcgcgtcca cgagcacgga gaaaggaatt cgattctgct ttgcgagcgc
     2641 ggcggcagtc acaatcgcct gccgccgaat tctcccatcg cgatgtttca ggcgttccgc
     2701 gagcttgggc gccagcgttt cttccttaag gcgtctaact tgcttgttgt tccacagtgc
     2761 atcaaacacc aatccgcctt cgagatcttc tgggcgagcc tccacaatct tgtatccgct
     2821 catccaacgg ggctcgtagc gaatagctcg ctctgtcgtc aggaggtata gcgtgcggcc
     2881 agcgcaagac caatgccaag ccaaggtgcc gactatcggt ccgctagata cgagcagaga
     2941 aagcgcccaa accccaatcg tgaccgcaga caagctgaca ctctgcggat cggcgcgaga
     3001 agccacaaaa cttaacaggc caacataaac aacggagttc aggccgagca tcaggagcgc
     3061 gtacttagcg tagagtatgc ggcttggctg accccaccag accagcttct cgccaggctg
     3121 gaccagataa ctcaccagcg actgaaacct acgcggaacc ggctggggtt ctacgcacat
     3181 cggtctctac ccgtcaatcg cctttagccg accggtgctg tcgccgaggc tgggaacgcc
     3241 ggcgtccatc cggtcgagga gggagaggaa gaggttgttg agcggcgctt cgccgacgtg
     3301 gatgtgacgg ccggccggaa tcgtgccgcc gcccttgccg gcgaggatga tcggcaggtc
     3361 gtcgtgccgg tggcggttgc cgtccgaaag gccgctgccg tagagcacca ggcaattgtc
     3421 gagcagtgtg ccgccgccgt cgggcgtcgc ttgtagcgtc ttgaggaagc gggcgaattc
     3481 cgtcaccagg aacaggtcga tcttcttcag ctggtccacc ttgtcttgct cgctgcggtg
     3541 gtgcgacagc tcgtggtggc cgttgttcac gccgaccatg cgatacggcc ggttggaacc
     3601 ctcgttggcc gtcatgaacg taatgacccg cgtcgtatcg gtctggaagg ccaggaccat
     3661 caggtcgtac atcaggcgga tgtgctcttg cacttcgccc ggcaggcccg tcggcacggc
     3721 gaagtccttc gggggctcgg ccggcggcgc ggtcgccgcg cgggtgatcc gcagctcgat
     3781 ctcgcggacg ctggcgaaat actggtcgat cttctgccgg tccgtctggc ccaggcggtt
     3841 cttcaggtcg gcggcctgct ccgaaacgac gtcgagcacg ctctgccgca cgcggctgcg
     3901 gcgggtctgg ccgccggaga gcgagttgcc gaacaaccgc tcgaacacca gccgcgggtt
     3961 gatctccttc gccatcggcg tcgtggcgtt cttccaggag atgttgttgg aataggcgca
     4021 gctatagccg ctgtcgcagc tgccggcgtc gcggccccgc tcgattccga tctcgagcga
     4081 aggcaggcga gtgagatggc cgatccgctc ggccgcggcc tgatcgacgc tctggccgac
     4141 gcggatgttc gcgccggacg tcttgaccgg atgcgcgccg gtcagcagcg acgcgctgga
     4201 gcgggcgtga tcgccggcgc cgtcgccctt ggcaaagccg ttctcctggg ccagaccgga
     4261 gaagaccgta atctgcgact ttacgtcggc cagcggctgc atggtcgggc cgaactcgaa
     4321 cgactccccc tctcccttcg gacgccaggc atccatgatc gcgccgttgg ggaacgagat
     4381 gaacgccagt cgcaccggcg gcctatttgc cgcgtcggcg gcaaaaccgc gggccggcct
     4441 cattgcctcg agaagcggca gccccagcgc gcaacccagg cctcgcagca ccgtccgccg
     4501 gctgatcgtc ttgctcacgg tgctgctcct tcgcctcggc gcttccggaa ggggtcgctg
     4561 tctacgacgg ccgaaacgag cgcggaaaac cgaaagtccc ggctctcgac ctcatccacg
     4621 attttatcga ccgcgcagcg gtcggggggc gacaattcgc ggcccaagcc aaaagttaac
     4681 attttttccg ccagacagcg agcgaattcc ccccgccgct tgtcgagcac gcgggccagc
     4741 tccagcg
//
LOCUS       DASRJB010000084        14464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1999359, whole genome shotgun sequence.
ACCESSION   DASRJB010000084 DASRJB010000000
VERSION     DASRJB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999359"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFB80_02410"
     CDS             complement(<1..227)
                     /locus_tag="VFB80_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32634.1"
                     /translation="MTLRYKLRTLLIALAVLPPLLWIGWTKYEAWKAEQTRLKALREV
                     QQQTIQFRSFFRPGSSAPPQYVPPTTSFIPA"
     gene            complement(231..488)
                     /locus_tag="VFB80_02415"
     CDS             complement(231..488)
                     /locus_tag="VFB80_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32635.1"
                     /translation="MFRFAIRDVLWLTVVVALSVGWWLDNRRNGFDRVRAAQTEANLR
                     EDKFWAEQRAIKLYEQRVQILRAAADRLPPTDFRGVAAEAN"
     gene            complement(499..687)
                     /locus_tag="VFB80_02420"
     CDS             complement(499..687)
                     /locus_tag="VFB80_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32636.1"
                     /translation="MRFRLRTLLIVLALAQVAIAVGAFNLARVVMAARERARHTQCNN
                     TLRLAIPIYTGPRPPPSP"
     gene            complement(1100..1873)
                     /locus_tag="VFB80_02425"
     CDS             complement(1100..1873)
                     /locus_tag="VFB80_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32637.1"
                     /translation="MSTAVPRSRRPWPRWIAVTFWTVLGLLAAALLVVMLTITLGAVH
                     GIEFSPERFERRSYSFYELPFVGIQVRAVRREDLTTVAETFITSNKYIPPPPPVGQET
                     WHIVIGSRGTRLMRRGDASILVQYLDAQDTKAMHRWVKWSEDHPQLAKVFWPAVQQLA
                     RHELYVFLPELFEMTKVHSDPVALQAALNAQVVQRLLFLARRLQDRDAHKQALAVLAD
                     ALALDPANQELQRAQNTSRAALPPAEAPPAKQPAAPTKK"
     gene            2040..2630
                     /locus_tag="VFB80_02430"
     CDS             2040..2630
                     /locus_tag="VFB80_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32638.1"
                     /translation="MRFLSLRAMFLLAFVIAMPVLALPPVARWLDEVIHGPSPTDFGR
                     PPGVSDAAPTEPIVAESVAPAGHFENSPAQSPQGLDTASAAAPPLAPPLAFAPVVPPT
                     SPPEPDPVIEKMALARLQQIRERLEELGADYVVVDSLDGAAGYRCLCRMLVDPRSRFT
                     RPFEATAADPLTAGELVLREVEAWHMASVQKRAAPR"
     gene            2713..3528
                     /locus_tag="VFB80_02435"
     CDS             2713..3528
                     /locus_tag="VFB80_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HZN32639.1"
                     /translation="MLSRAQTWWMILRIALQERLVYRGDFALGTLMRFLPIVTQIFLW
                     MAVFAARQGDQLAGYSYRDFVAYYLLTMVARAFSSMPGLSSGIAAQIRSGEVKKFLIQ
                     PVDLLGFLLLGRVAHKLAYYTVATLPFALVFFLCRGFFPGWPSATALAAGVLSLVLCF
                     LLGYFLEAALGLVGFWWLEVSSLLFVYMLFSFFLSGHMFPLDMLPQPWHTLVELTPLQ
                     YLAYFPAAVLLGKIEGWDLVRGLCIEAGWVLFFALLARVLWHYGTRHYSAYGG"
     gene            3530..4387
                     /locus_tag="VFB80_02440"
     CDS             3530..4387
                     /locus_tag="VFB80_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HZN32640.1"
                     /translation="MSPRPDYFRVFLTFAGNSLVREMMFHVNFLIECVSSVTWTMMNL
                     GFYLLIFHHTDSIGYQTGWGKWEFFAFLATTMLVNSIVQAFFMPNCEDISELIRTGGL
                     DFALLKPIDTQFLISLEKIDWSSLANFAAGLVLLIASLIQMTTRAENPLTLHPLSLVL
                     YVFYVLCGVAILYSLMIALSATSIWLGQNRSLYDFWFYITNFSRYPMEIYEGRLGTPL
                     RWFCTYIVPVLIVVNVPAQIIARPISPDGSTSVWPLAGFTVIATAASLIASRWVFQAS
                     LRSYRSASS"
     gene            5004..5498
                     /locus_tag="VFB80_02445"
     CDS             5004..5498
                     /locus_tag="VFB80_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN32641.1"
                     /translation="MANHAPSAAGRKQGRRFDSPQQEVYLSLWRTYDRLRMIEDELFL
                     QHDLTAQQYNALRLLKAEHPGTLPTLVLAGRLVSHAPDITRLVDRLQDRGLVARQRMP
                     DNRRVVQVGITDAGLALLKELEQPVRECHLKQLGHLPPAQLQSLVELLRIARQPHEDE
                     GSPW"
     gene            complement(5502..6674)
                     /locus_tag="VFB80_02450"
     CDS             complement(5502..6674)
                     /locus_tag="VFB80_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN32642.1"
                     /translation="MSYESHPPGRQPRSLTSLVPLLVVLVAALVVLQLWGLFNRGGLP
                     RFALDPAAEPRPIAPAGDLAADEKATISLFKQSSQSVVHITTATLGRDFALNIEEIPR
                     GSGSGFLWDEKGHIVTNYHVVENSNRFKVTLADQSTWDAVGIGAAADKDLAVLRIDAP
                     AERLRPLLVGTSADLEVGQKVFAIGNPFGLDQTLTTGVISGLGRQIQARTGRTIDGVI
                     QTDAAINPGNSGGPLLDSRGRLIGVNTAIFSPSGTSAGIGFAIPVDTVQRIVPQILRQ
                     GDVRRPSLAAEFFPDPLVRQLRIKGALVSRIAPSGPAAQAGLRPSRATSDGDVNWGDL
                     IVAVDGKPVETSEELLAAIESRDVGQRVQLSVVRGLGTGRQQTLDVPVTLAPEPGE"
     gene            6811..9606
                     /locus_tag="VFB80_02455"
     CDS             6811..9606
                     /locus_tag="VFB80_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZN32643.1"
                     /translation="MLKVRRGARPSWIWPTLLLATAPLIAHAQPASNAPPRVIGFERF
                     YAPAGSDAEAAGNLLLGELNCTSCHQTDTGRTPYIHKKQAPVLDSVCSRVKPQYLLKF
                     LADPQATKPGTTMPGVLAGVPEGERGPIVESLVHFLATTGTVLHSNPMRQQVARGEML
                     FHSAGCVACHDPRKEPALQPLATSIPIGTPSRKYTLPGLTQFLENPLAVRPGGRMPHL
                     NLSSAEARDIASFLLNDLDIASGLQYAYYEGSWDELPDFAKLTPKATGDAANFDVSVA
                     KRRDGFGLRFDGTINLPKDGDYLFLIGSDDGSRLLIDEKVVVDNGGIHPFQQKRKRVK
                     MTAGLHTVAVEYFEQGGDEALQVDFEGPGMPQMPLATLLAAPVTTKGTASPLEEFTVD
                     AAKAARGREHFAALGCAACHALQIGGQAVAPTKTAKPLAGLSLTQRTGASCLSETANG
                     TPRYALSPRQKEVLTAAIAASKQQPRDLGAGQIVQQTLVRFNCYACHDREQVGGVEEA
                     RNAFFQSDMPEMGDEGRLPPHLTGVGAKLQVEWLNAVLENGAKDRPYMFTRMPEFGLA
                     NVGTLVAGLAKADAGAIRPAPETKVAPGDEEKRLKAAGRRLVGSQGLSCIKCHTFAGK
                     RSTGIQALSLTTMTRRLRPEWFHHYLQNPLAYRPGTRMPTSFPDGKAVLSSVLEGSVE
                     KQIAAMWSYLEAGDQAILPVGLVTGKIELMAFDEAVIYRNFIEGAGTRAIGVGYPDKL
                     NLAFDANESRLAMLWHGGFIDANKHWNGRGAGFEGPLGDQILRLPDGPPLAALDDTAA
                     EWPKRPAQELGYQFRGYRLVEKRNPVFLYSASGLEVEDYPRPVGSQDVFVMQRTITIA
                     APPRGEKPPANLWLRAIRAGQIEPAGDNFKIDGKWDLKIASTTKPHIREQGGQSELLV
                     PIAFDGNTAKIELTYDW"
     gene            9686..11182
                     /locus_tag="VFB80_02460"
     CDS             9686..11182
                     /locus_tag="VFB80_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32644.1"
                     /translation="MRRYASLISLLLVLALCATALAQRGRRGERDGSPEDEYYRLIRF
                     PMSDQIVLEAGALEWLPDGKLAVATRRGDIYLVDQPLTDKPDEATFKQYASGLHEILG
                     LAWKDGWLYCVQRCEVTRMKDDDNDGRADRFETVSDGWGITGDYHEYAFGSKFDKGGN
                     LWVTLCLTGSFTSDAKYRGWCLRITPEGKVIPTTSGLRSPGGVGANAEGDMFYTDNQG
                     PWNGACALKWLRPGGFVGHPIGNRWYDVAGEVMGPRPAEPLSGSRMMVEAKKIPQLEP
                     PAIYFPYNKMGQSAAGIACDLSSGKFGPFGKQLFVGDQTHSTVMRVALEKVQGHYQGA
                     CFPFRAGFGSGSLSLLFAPDGSLFVGGTNRGWGSRGSQPYSLDRLVWTGKIPFEIHEM
                     HARPDGFELTFTEKVDPATAGDVKSYSASTYTYIYQSAYGSPEVDATTPQITKIDVRP
                     DGQGVRLFLDKLQEGHVHELHLDGLRSASGAPLLHPAAYYTLNYIPDP"
     gene            11328..12833
                     /locus_tag="VFB80_02465"
     CDS             11328..12833
                     /locus_tag="VFB80_02465"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZN32645.1"
                     /translation="MHPAPAGAEVSLRDILPKARFLAHSDVRAASCSADWQACRPGDI
                     YFALTTADDDGHEHAPAAIERGAVAVVAERMLPVETPQVLVRDTRAAFARVCQALAGH
                     PSRELTTIGVTGSSGKTVTSILLASVLEAAGLSAGVLSSIGHSDSVVQRVAPSGTPTA
                     AEFAAWLRRMKSAGSQSAVLELSSRALAERRTSGLALDAAVLTNIQTEHLDEHNSPRA
                     YAQIKRRIFRHLKPGGVAVVCADDHRCRNLLPLLGGAALTYALHAEADITGQVLERSL
                     CEQTFMITAGGDSAPVRTRMIGDHHVANCLAATAVGLSLGLELQDIVRGLEAVERVPG
                     RMERLECGQPFGVFVDAAATPETLSRSIATLRQVTPGRVLVVFGPRESADGARRAMLG
                     RVLERGCHLPILTSGEPGQTAPLAGIHELLDGFERPHKAQNLPDRASAIRFALSQAQA
                     GDAVLIAGRGERGSELKGRQKLTHDDREIACAWLYEPQPPENCRPRFRVVG"
     gene            12908..13363
                     /locus_tag="VFB80_02470"
     CDS             12908..13363
                     /locus_tag="VFB80_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32646.1"
                     /translation="MHLCLLDLLEFTGGRLRLSETPPHAGDLATIERICLAADDVQPG
                     DVYWCLGAGPCDVELAFLRGALGVVAATACQPWPGRFSLAVEDTVFALERLVDGLRRA
                     AAGEQESSAGSPELKDLQLPATESAANFPPTCGRLGESRSAARCRRIAA"
     gene            13714..>14464
                     /locus_tag="VFB80_02475"
     CDS             13714..>14464
                     /locus_tag="VFB80_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32647.1"
                     /translation="MKRTLSIAAIFAILMPATALAATLRGEIRDEASGELIAARIYIQ
                     SDKGEWFLARSAAADGKAVTYKRSRGDSTEVHTALSAHPFEAELPAGKYTITVEKGKE
                     YLAHEEQVTLADAGAKLDVRLRRLVNMADRGWYSGDTHVHRPLDELPTLMLADDLNVA
                     FPLTYWVTKSDTPPSQGDKNQPAVAAKLIEVAPRHVIWPLNTEYEIFTVGERRHTLGA
                     VFGLGHQTVLQRGAPPVRPIAEQVHAEGGLLD"
BASE COUNT         2476 a         4637 c         4699 g         2652 t
ORIGIN      
        1 tctgccggga taaaggaagt cgtcggcggg acatactgcg gcggggcact cgacccaggt
       61 ctaaaaaatg atcggaattg aatcgtctgc tgctgcacct cgcgaagcgc cttgagccgt
      121 gtttgttccg ccttccacgc ctcgtacttc gtccagccga tccacagcag cggcggcagg
      181 accgccaagg cgatcagcag cgtgcgaagt ttgtagcgga gggtcatcgt ctagtttgcc
      241 tccgccgcca cgccgcgaaa gtccgtggga ggcagcctat cggccgccgc tcgcagaatc
      301 tgtacgcgct gctcataaag cttaattgcc ctttgttcgg cccagaattt gtcttcacgg
      361 agatttgcct cggtctgggc agctcgcact cggtcaaagc cgttccttcg attgtccagc
      421 caccacccca ccgacagcgc caccaccacc gtcaaccaca gcacgtcgcg gatcgcgaat
      481 cggaacatgg gtgggttcct agggggatgg tggcggtcgc gggccggtgt aaatcggaat
      541 agccagtcga agcgtgttgt tgcactgcgt gtgcctggct cgctcgcggg ccgccattac
      601 gacgcgtgcg agattgaatg ccccaacagc aatcgcgact tgggccagcg ccaagacgat
      661 cagcagcgtg cggaggcgga aacgcatcag gtcagcctat cgctttcgcc ggtcgggcgt
      721 caaacgaggc tggcggcggg tagtgtccta atttgatttc ttgagcttgt agtggatgcc
      781 gtcaggtatg aactcgatct cggtgttttt gttactgcca ttaattcccg gcgctccaga
      841 ttcaagttgt tgtgcagaat caggatccgc gcatgagccg cgggggcgcc ccctggcagc
      901 tccacgcgcc cgcgaatcag aaggggcgga gaagcagcgt gtgccgccac gccggtaact
      961 agcagcaagc cggcgccgat caaccagtgg acgcggctag tcataattat ccaaagtcca
     1021 aagtcgaagg tccgaagtca agtgtgtgaa tcgtctgcgg gagccggttc gttggtaggg
     1081 ggaatgggcg cagcggcagt cacttcttgg ttggcgctgc gggctgtttg gccggcggcg
     1141 cttcggcagg cggcagggca gcgcgggagg tgttttgggc ccgctggagc tcttggttgg
     1201 cggggtcgag cgccagtgca tcggccagga cggcaagggc ctgcttgtgg gcgtccctgt
     1261 cttgcaggcg ccgggcgaga aacagcagcc gctggacgac ttgagcgttg agtgctgcct
     1321 gaagagcgac gggatcggag tggaccttcg tcatttcgaa cagctcgggc agaaacacgt
     1381 acagctcgtg ccgcgcgagc tgctgcacgg ctggccagaa gaccttggcc agttgcgggt
     1441 ggtcttccga ccacttcacc cagcggtgca tcgccttggt gtcttgggca tccaggtatt
     1501 ggaccaggat gctggcgtct ccgcgccgca tcagccgcgt gccgcgcgag ccgatcacga
     1561 tgtgccaggt ctcctgaccc acaggcggcg gcggagggat gtacttgttg gaggtgatga
     1621 atgtctcggc aaccgtggtc aggtcctcgc ggcgaacggc tctcacctga atgccaacaa
     1681 acggcagctc gtaaaaggaa taggagcggc gctcgaatcg ctccggcgag aactcgattc
     1741 cgtggaccgc ccccagcgtg atcgtgagca tcacgaccag cagcgcagcg gcgagcaacc
     1801 ccagcaccgt ccaaaaggtg acggcaatcc agcggggcca ggggcgtcgg ctgcgcggga
     1861 cggcagttga catcgcttcg ctcgcggaag attggatcgg caactatatg ttcgctttcc
     1921 gtgccagcgg gggcaacgcc tcgtgctagc agaagcgtgt tcgcctttgc actggcggga
     1981 acctggacta gaatcctgcc gcactggaac gccgaattgc cactgccagg gaaggcctga
     2041 tgcgatttct ttcgctgcga gcgatgtttt tgctggcgtt tgtgatcgcg atgccggtgc
     2101 tggcgctgcc gccggtcgcg cgctggctgg acgaagtcat ccacggcccg tctccgacgg
     2161 attttggccg gccgcccggg gtcagcgacg ccgcccccac ggaaccaatc gtagccgaga
     2221 gcgtggcccc cgcgggacac ttcgaaaaca gcccggcgca gtcgccgcag ggcttggaca
     2281 ccgcctccgc tgccgcaccg ccgctggctc cgcccctggc ttttgctccg gttgttccac
     2341 cgacgagtcc cccagaaccg gatccagtca ttgaaaagat ggcgctggcc cgcttgcagc
     2401 agattcgcga gcgactggag gaactgggcg ccgactacgt cgttgtcgac agcctggatg
     2461 gcgcggccgg ttatcgctgc ctgtgccgca tgctcgtgga tccgcggtcc cgattcacgc
     2521 ggcccttcga ggcgacggcc gccgatccgc tgaccgccgg cgagctggtc ctgcgcgaag
     2581 tggaggcgtg gcatatggcg agcgtccaga agcgtgccgc gccgcggtaa ctcggttatc
     2641 agtcaaccac ctcgggcgga aggctgccat gcccactttc cagcgcctga cgagcggcta
     2701 gcatcaagcc tgatgttgtc ccgcgctcag acctggtgga tgatcctccg catcgcgctg
     2761 caagagcggc tggtgtatcg gggggacttc gcgctcggca cgctgatgcg gttcctgccg
     2821 atcgtcacgc agatttttct gtggatggcg gtctttgcgg cgcggcaggg agatcaacta
     2881 gcgggctaca gctaccggga cttcgtggcc tactacctgc tgacgatggt ggcccgcgcg
     2941 ttttcgagca tgccggggct ctcctcagga atcgccgccc aaattcgcag cggcgaagtc
     3001 aaaaaattcc tgatccagcc cgtggatttg ctgggctttc tgctgctggg ccgcgtcgcc
     3061 cataagctgg cctattacac cgtggccacg ttgccgtttg ccctggtctt ttttctgtgc
     3121 cgcgggtttt tccccggctg gccgagcgcc acggcgctgg cggcgggagt gctttcgctc
     3181 gtcctctgtt ttctgctcgg atatttcctg gaggctgcgc tggggctggt tgggttctgg
     3241 tggctggagg tcagctcgct gctgttcgtc tacatgctct tcagcttttt cctttccggg
     3301 cacatgttcc cactggatat gttgccccag ccctggcaca cgctggtgga gctgacgccg
     3361 ctgcaatacc tggcttactt tcccgcggcg gtgttgctgg gcaagattga gggttgggat
     3421 ttggtgcggg gcttgtgcat tgaggcgggc tgggtgctct tcttcgctct gctcgcccgg
     3481 gtcttatggc actacggaac gcggcattat agcgcctatg gaggctgata tgtcgccgcg
     3541 ccccgattat ttccgcgtgt ttctaacatt cgcgggcaac agcctggtgc gggagatgat
     3601 gtttcatgtc aatttcctca tcgaatgcgt tagcagcgtg acctggacga tgatgaactt
     3661 gggcttctac ctgctcatct ttcatcatac cgattcgatc ggctatcaaa ccggctgggg
     3721 caaatgggag ttctttgcgt tcctggcgac gacgatgctg gtcaacagca tcgtgcaggc
     3781 cttcttcatg ccgaattgcg aggatatcag cgagctgatc cggacgggcg ggctggattt
     3841 cgccctgctc aagccgatcg acacgcagtt tctgatctcc ctcgagaaaa tcgattggtc
     3901 gagcctggcc aattttgccg ccggcctggt gctattaatt gccagcctga ttcaaatgac
     3961 gacgcgggcc gagaatccac tcaccttaca tcccttgtcc ctggtgctgt atgtcttcta
     4021 tgtactctgc ggcgttgcca tcctatacag tttgatgatc gcactgtcgg cgacgagcat
     4081 ttggttgggg cagaatcgct cgctctatga tttctggttc tatatcacga atttctcgcg
     4141 ctacccgatg gagatctatg aagggcggct gggtacgccg ctgcgctggt tctgcaccta
     4201 tatcgttccg gtgctgattg tggtcaacgt gccggcccag atcattgccc ggccgatttc
     4261 gcccgatgga tcgacgtccg tctggccctt ggccggcttt acggtgattg ccacagccgc
     4321 cagcctgatc gcatcgcggt gggtctttca ggcctcgctg cggagctatc gcagcgccag
     4381 ctcctagtgc caccgctcga cttgcgcccg cgagttcttc gccggatcgg gcagctcggt
     4441 ggcgaacaat tcggccgcgg ttggcgaaat tcgcaggctt ttccgcgttt tgggccgcga
     4501 attgttcgcc aatctgcctc gataccagcg gatctggcga tcaattcact gccgccggcc
     4561 gcaagtgcct tgcggcatca gcagttacga gcgaaatgcg ggtggcgaac aattcgccgc
     4621 acgctcgccc tgcaaaccgc cgcgagtatc agcgattggc ctctgtgagt gccgctgccg
     4681 gcttgcccgc agtgccgacc atttctcgca ggccacccac gccaatgctc gcgaccggct
     4741 tgcgcgtcgc ccgccacgaa tttccaaaga acatcccgcc gtcacacagc gacagcgagt
     4801 tgcacttgta tctgtcagtc catcatctat caagtcagtc aagaatcgcg gtgatctcta
     4861 gtgaacagat tgtagcatcc gacccccgcc tcgagccctc agtagagggg tcccggcgtg
     4921 aagattttcc aacttcgcct gggagacgca cgacgcctct agtttgtctc cacaatgctt
     4981 gttcgaccaa ctaaactggc gacatggcca atcacgcccc gtccgccgcc ggccgcaagc
     5041 agggccgccg tttcgattcg ccccagcaag aggtctacct cagcttgtgg cggacctacg
     5101 atcgcctgcg gatgatcgag gatgagctgt ttcttcagca cgatctgacg gcccagcaat
     5161 acaacgcctt gcggctgctc aaggcagagc accccggcac gctgccaact ttggtgctgg
     5221 ccgggcggtt ggtgtcgcac gccccggaca tcacgcggct ggtcgatcgg cttcaggacc
     5281 ggggactcgt tgccaggcag cgcatgcccg acaaccgccg cgtcgtgcaa gtcggcatca
     5341 ccgacgccgg gctggcgctg ctcaaggagt tggaacagcc ggtgcgcgag tgccacctga
     5401 agcagctcgg gcacttgcct cccgcgcagc tgcaatcgct ggtcgagcta ttgcggattg
     5461 cccgccagcc gcacgaagac gaggggagcc cgtggtgaga gtcattcgcc gggttcgggc
     5521 gcgagcgtga ccgggacgtc cagcgtttgc tggcggccgg tgcccaagcc gcggacgacg
     5581 ctaagctgca cgcgctggcc gacgtcgcgg ctctcaatgg ctgcgagcag ctcttcggac
     5641 gtctccacgg gtttgccatc gaccgcgacg atcaagtcgc cccagttaac gtcgccgtcg
     5701 gacgtagcgc gcgaggggcg cagcccggct tgagcggccg gcccactcgg agcaattctg
     5761 ctgaccagcg cgcccttgat gcgcaattgg cggactagcg gatcggggaa gaattcggcg
     5821 gccagcgagg gccgccgcac gtcgccttgg cgcagaatct gcggcacgat ccgctgcacg
     5881 gtatcgaccg ggatggcaaa gccgatgccg gcgctcgtgc ccgacgggct gaagatcgcc
     5941 gtattgactc cgatcagccg gccgcggctg tcgagcagcg gtccgccgga gtttccaggg
     6001 ttgatcgcgg cgtcggtctg aatcacgccg tcgatcgtgc ggccggtgcg ggcctggatt
     6061 tgccgaccta atccgctgat tacgccggtg gtgagcgtct gatcgagtcc aaacgggttg
     6121 ccgatcgcaa agaccttctg cccgacctcc agatcggccg aagtgccgac gagcagcggc
     6181 ctcagccgct cggccggcgc gtcgatccgc agcacggcca ggtccttgtc ggccgccgcc
     6241 ccgattccca ccgcatccca ggtgctttga tcggcgagcg tcaccttgaa acgattggag
     6301 ttttccacga cgtggtagtt ggtgacgatg tgccccttct cgtcccacaa aaaaccgctg
     6361 cccgagccgc gggggatttc ctcgatgttg agtgcgaaat cgcggccgag cgtggccgtg
     6421 gtgatatgga ccaccgactg cgacgactgc ttgaacaggc tgatcgtcgc cttttcatcc
     6481 gcggccaggt cgccggccgg cgcgatcggc cgcggctcag ccgccggatc gagcgcgaac
     6541 cgcggcaacc cgccgcggtt gaacaacccc cagagttgca gcacgacgag cgccgccacg
     6601 agcacgacca gcagcggcac gagcgacgtg agcgaccgcg gctgccggcc gggcggatgc
     6661 gactcgtagg acatggtttt tctcgccttg tatccgattc gtctaatcta atgctgcgac
     6721 cgttgacatt gacaccccac gcaatccata aaggaaacct gcattttttc tgcccgccat
     6781 ttgcccgccg ctggttgctg gagtctgtcg atgctcaagg ttcgccgggg tgctcgcccc
     6841 tcttggattt ggccgactct gctcctggcg accgcaccgc taattgctca tgcgcagccc
     6901 gcgagtaacg ctccgccgcg tgtcatcggc ttcgagcggt tctatgcgcc ggccgggagc
     6961 gacgccgagg cggccggcaa cctgctcttg ggcgagctga attgcacgag ctgccatcag
     7021 acggacaccg ggcggacgcc ctacatccac aaaaagcaag cgccggtgct cgacagcgtg
     7081 tgcagccgcg tcaagccgca atatctgctc aagttcctgg cagatcctca ggccactaag
     7141 ccgggcacga cgatgcccgg cgtgctggcg ggcgtgccgg agggggagcg tgggccgatc
     7201 gttgaatcgc tcgtacattt tctcgcgacg accggcacgg tgctgcactc caacccgatg
     7261 cggcagcaag tggcccgcgg cgagatgctg tttcactccg cgggctgcgt ggcctgccac
     7321 gatccgcgga aggagccggc gttgcagccg ctggcgacgt cgattcccat tggcacgccg
     7381 tcgcggaaat acacgctgcc cggactgact cagttcctgg agaatccgct cgccgtgcgt
     7441 ccgggcggcc ggatgccgca tttgaatctg tcgtcggcgg aagcccgcga catcgcctcg
     7501 ttcctgctga acgacctcga catcgcctcg ggcctgcaat acgcctacta cgagggctcg
     7561 tgggacgaat tgccggattt tgcgaagctg acgccgaagg cgacgggcga tgcggccaat
     7621 ttcgacgtca gcgtggccaa gcgccgcgac ggctttgggc tgcgattcga cggcaccatc
     7681 aacctgccca aggacggcga ctacctgttc ctcatcggtt cggacgacgg ctcgcggctc
     7741 ttgatcgacg agaaggtcgt cgtggataac ggcggcatcc atccgttcca gcagaagcgg
     7801 aaacgggtga agatgacggc cggcctgcac acggtcgcgg tcgagtactt cgagcagggc
     7861 ggcgacgaag cactgcaagt cgatttcgaa gggccaggga tgccgcagat gccgctggcg
     7921 acgctgctgg ccgcgccagt tacgacgaag ggcaccgctt cgccgctgga ggagtttacc
     7981 gtcgatgccg cgaaggccgc ccggggccgt gagcacttcg cggcgctcgg ctgtgccgcg
     8041 tgccatgcgc tccagatcgg cggccaggcg gttgccccga cgaagacggc caagcctctg
     8101 gcggggctgt cgctgaccca gcgcaccggc gcgagctgct tgagcgagac agccaatggc
     8161 acgccgcgct acgcgctcag cccgcgacag aaggaagtgc tcacagcagc gattgccgct
     8221 tccaagcagc agccccgaga cctgggcgcg ggccagatcg tgcagcagac actggtgcgt
     8281 ttcaactgct acgcgtgcca cgatcgcgag caggtcggcg gcgtcgaaga ggcccgcaac
     8341 gcgttcttcc agtccgacat gccggagatg ggggacgaag gccgcctgcc gccgcatctg
     8401 actggcgtcg gtgcgaagct ccaagtggaa tggctcaatg ccgtattgga aaacggggcg
     8461 aaagaccggc cgtacatgtt tacgcgaatg ccggaattcg gactcgccaa cgttggcacg
     8521 ctggtcgccg gcttggcgaa ggccgacgct ggtgcgatca ggcccgcgcc ggagactaag
     8581 gttgcccccg gagacgaaga aaaacgcctg aaagccgccg gacgccggct ggtgggatcg
     8641 cagggtctct cgtgcatcaa gtgccacacg ttcgccggca agcgctcgac cggcattcag
     8701 gccctcagcc tgacgacgat gaccaggcgg ctgcgaccgg aatggttcca tcattatttg
     8761 cagaacccgc tggcctaccg gccgggcacg cggatgccca cgtcgtttcc cgatggcaaa
     8821 gcggtgctgt cttcggtgct ggagggaagc gtcgagaagc agatcgcggc catgtggagc
     8881 tacctcgagg ccggcgacca ggcaattctc cccgtgggac tggtcaccgg aaaaatcgag
     8941 ttgatggcct tcgacgaggc ggttatctac cgcaacttca tcgaaggtgc aggcacgcgg
     9001 gcgattggcg tcggctatcc cgacaagctc aacctggcgt tcgacgccaa cgaaagccgc
     9061 ctggcgatgc tctggcacgg cgggttcatc gacgccaaca agcactggaa cggccgcggc
     9121 gcgggcttcg agggcccgct cggcgatcag atcctccgcc tgcccgacgg accgcccctg
     9181 gccgcgctgg atgacacggc cgcggagtgg ccgaaacgcc cggcccaaga actcggctat
     9241 caattccgcg gctaccgcct cgtcgaaaaa cgcaacccgg tgttcctcta ctccgctagc
     9301 ggcttggaag tggaagatta cccgcggccc gtcggttctc aggacgtctt cgtcatgcag
     9361 cggacgatca cgatcgccgc gccgccgcgc ggcgagaagc cgccggccaa tctctggctc
     9421 cgcgccatcc gcgccggcca gatcgagccg gcgggcgaca acttcaagat cgacggcaaa
     9481 tgggacctaa agatcgcctc gaccacgaag ccgcacattc gcgagcaagg cgggcagtcg
     9541 gaactgctgg tgcccatcgc cttcgacggc aacaccgcaa agatcgaact cacatacgac
     9601 tggtaaccct gactctcgtt cccaggctcc gcctgggaac gcacttccgc gaggctcccg
     9661 cctcgtgccc tggagtagat ccagaatgcg acgatacgcc tcactgatct ccctgcttct
     9721 cgtccttgcg ctctgcgcca ctgccctggc tcagcgcggc cgccgcggcg agcgcgacgg
     9781 cagtcccgag gacgaatact accgcttgat ccgctttccg atgtcggacc agatcgtcct
     9841 ggaggccggc gcgctcgaat ggctgcccga tggcaagttg gcggttgcca cgcggcgcgg
     9901 cgacatctac ctggtcgatc agccgctgac ggacaagccc gacgaagcga ccttcaagca
     9961 atatgccagc ggcctgcacg aaatcctcgg actggcgtgg aaggacggct ggctgtactg
    10021 cgtgcagcgg tgcgaagtca cgcggatgaa ggacgacgac aacgacggcc gcgccgaccg
    10081 cttcgagacc gtcagcgacg gctggggcat cacgggcgac tatcacgagt acgccttcgg
    10141 aagcaaattc gataagggcg gcaatttgtg ggtgacgctc tgcctgaccg gctcgttcac
    10201 cagcgacgcc aaataccgcg gctggtgctt gcggatcacg ccggagggca aagtcattcc
    10261 cacgaccagc ggcctgcgtt cgccgggcgg cgtcggcgca aatgccgagg gggacatgtt
    10321 ctatacggac aaccaaggtc catggaacgg cgcgtgtgcc ctcaagtggc tgcgtcccgg
    10381 cgggttcgtc ggccatccga tcggcaaccg ctggtacgac gttgcgggcg aggtcatggg
    10441 cccgcggccc gccgagccgc tcagcggcag ccggatgatg gtcgaagcca aaaagatccc
    10501 gcagctcgag ccgccggcga tctattttcc ctacaacaag atggggcaat cggcggcggg
    10561 aatcgcgtgc gacttgagta gcggcaaatt cggcccgttt gggaagcagc tcttcgtcgg
    10621 cgatcagacg cacagcacgg tgatgcgggt cgccctcgaa aaggtgcagg gccactacca
    10681 aggcgcgtgc tttccgttcc gcgccggctt cggcagcggc tcgctgtcgc tgttgtttgc
    10741 gcccgacggc tcgctcttcg tcggcggtac caatcgcggc tggggctcgc gcggctcgca
    10801 gccctactcg ctcgaccggc tggtttggac cggcaaaata ccgttcgaga ttcatgaaat
    10861 gcacgcccgg ccggacggct tcgagctgac gttcaccgag aaggtcgatc cggcaaccgc
    10921 gggcgatgtg aagtcctact cggcgagcac ctacacgtat atctatcagt ccgcctacgg
    10981 cagccccgag gtcgatgcca ccacgccgca gatcaccaag atcgacgtgc ggccggacgg
    11041 acagggcgtg cggctatttc tcgacaagct ccaggagggg cacgtccacg agttgcatct
    11101 ggacggcctg cgttcggcct ccggcgctcc gctgctgcat cccgcggcct attacacgct
    11161 gaattacatc cccgatccgt agttgcgcca attcgtcttg acgcgcggag cgcgatggga
    11221 acaattctcg tcgcgtgtgg ccgccaggag ggcggcgggt cagctagcgg gctgttcgct
    11281 agcggcctct cgcgcgaagc atccatccgc ggcaaggagg cccgaagatg cacccagccc
    11341 cagccggcgc agaagtctcg ctgcgcgaca tccttcccaa ggcccgcttt ctggcccaca
    11401 gcgacgtgcg ggccgcttcc tgctcggccg actggcaggc ctgccggccc ggcgatatct
    11461 acttcgcttt gactacggcc gacgacgacg ggcatgagca tgccccggcg gcgatcgagc
    11521 gcggcgcggt cgccgtcgtc gcggaacgga tgctgcccgt cgaaacgccg caagtgctcg
    11581 tccgcgacac gcgtgcggcg ttcgcccgcg tctgccaggc cctggccggc catcccagtc
    11641 gcgaattgac gacgatcggc gtcacgggct cgtccggcaa gaccgtgacg agcattttgt
    11701 tggcctcggt gctcgaagcg gccggcctga gcgccggcgt gctctccagc atcggccaca
    11761 gcgactcggt cgtgcagcgc gtggctccca gtggcacgcc gacggccgcg gagttcgccg
    11821 cgtggctgcg gcggatgaag tctgccggca gccagtcggc cgtattggaa ttgagcagcc
    11881 gggcgctggc cgagcgccgc accagcgggc tggccctcga tgcggccgtg ctgacgaaca
    11941 ttcagaccga acacctggac gagcacaact cgccgcgggc ctatgcccag atcaagcgcc
    12001 gcatattcag gcaccttaag cccggcggcg tcgcagtcgt ctgcgccgac gaccatcgct
    12061 gccggaactt gctgccgctg ctgggcggcg cggcgctgac ctacgccctg cacgccgagg
    12121 ccgacatcac cggccaggtc ttggaacgga gcctctgcga gcagactttt atgattaccg
    12181 cgggcggaga ctccgcaccc gtccgcacgc gaatgatcgg cgatcatcac gtcgccaact
    12241 gcctggccgc gacggcagtc ggcctgtcgc tgggcctgga actgcaggac atcgttcgcg
    12301 gcctggaggc ggtcgaacgc gtgccggggc gaatggagcg actggaatgc gggcagccct
    12361 tcggcgtgtt cgtcgatgcc gcggcgactc cggagacgct gtcgcggtca atcgccacgc
    12421 tgcggcaggt gaccccgggg cgggtgctgg tcgtgttcgg cccgcgggaa agcgccgacg
    12481 gcgctcgccg agcgatgctc ggccgcgtcc tggagcgcgg ctgccacctg ccgattctca
    12541 ccagcggcga gccggggcag actgctcctc tggcaggcat tcacgaactg ctcgacggct
    12601 ttgagaggcc gcacaaggcg cagaacctcc ccgaccgcgc ctccgcgatt cgctttgccc
    12661 tgtcgcaggc ccaggcaggt gatgccgtgc tgatcgccgg ccgcggcgag cgtggcagcg
    12721 agctgaaggg ccgccagaag ctcacgcacg acgaccggga gattgcctgt gcatggctgt
    12781 atgagccgca gccgccggaa aactgtcgtc cgcggtttcg agtcgttggt tgatcgaatt
    12841 tccaagaacc gaagaaccaa agaaccaaat tcggtagttc ggttcttggg ttctttgagc
    12901 atttcccatg cacctctgcc ttctcgatct tttggaattc accggcggcc ggttgcggct
    12961 gagcgagact ccgcctcatg cgggcgacct ggcgacaatc gagcgcatct gcctggcggc
    13021 cgacgatgtg cagcccggcg acgtctattg gtgcctgggg gctggtccgt gtgacgtcga
    13081 gctggcgttt ctgcgcggag cgctgggcgt ggtggccgcc acggcctgcc agccttggcc
    13141 ggggcgattc agcctggccg tcgaggacac ggtttttgcc ctcgagcggc tggtggatgg
    13201 gttgcggcga gcggcggcgg gcgagcaaga atcttcggca ggttccccgg aattgaaaga
    13261 tttgcaattg ccggccacgg agagcgccgc taatttcccg cccacctgtg gccgactggg
    13321 cgaaagccgc tcggccgcca ggtgccggag aatcgcggcg taagagagct tgccgcattt
    13381 gcctccggcc tcatcaccca atcaatgagg agcttcaccg gaggcagttg gtttgcggga
    13441 accggctgct gccctccctc ctgggttgaa gcatttaact tgccggcgga acttctgggt
    13501 cagttccgcc ggtgagtgct tcccggtgaa gctttttact gtgcgccggg gcggcgtcaa
    13561 aaccgctccg gcgccaggga acaacacgcg gggctgcgat cgcaccggtc gcagccccgc
    13621 tgttttttta ggcggccagc gtttcttggc cgattgccgg cgcggccgct gggggctacc
    13681 gtgagcgcca tggagcgctc cgggaggccg ctgatgaaac gcactttgag catcgccgcg
    13741 atctttgcaa tcctcatgcc ggctacagcg ctagccgcaa ccctccgcgg ggaaatccgc
    13801 gacgaagctt ccggcgagct gatcgccgcc cggatctata ttcaatccga caaaggcgaa
    13861 tggttcctcg ccaggtcggc cgcggctgac ggcaaggccg tgacctacaa gcgttcccgc
    13921 ggcgacagca cggaagtcca cacggcgctc tcagctcatc cgttcgaggc ggagctgccc
    13981 gccggcaaat acaccatcac cgtcgaaaag gggaaggagt atctcgcgca cgaagaacag
    14041 gtcacgctgg ccgacgcggg cgcgaagctg gacgttcgcc tgcggcggct ggtgaacatg
    14101 gcggaccgcg gctggtattc gggcgatacg cacgtccacc gtccgctgga cgaactgccg
    14161 acgctgatgc tggccgacga cctcaacgtc gcctttccgc tgacctattg ggtcacgaag
    14221 tctgacacgc cgccgagcca gggcgacaaa aaccagccgg cggtcgccgc gaagctaatc
    14281 gaggtcgccc cgcggcacgt catctggccg ctcaacacgg agtacgagat ctttacggtc
    14341 ggcgagcggc ggcacacgct gggcgcggtg tttggcctgg ggcatcagac ggtactgcag
    14401 cgcggcgcgc cgccggtgcg gccgattgcc gaacaagtgc atgccgaggg cggcctcctc
    14461 gaca
//
LOCUS       DASRJB010000085         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3128552, whole genome shotgun sequence.
ACCESSION   DASRJB010000085 DASRJB010000000
VERSION     DASRJB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128552"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFB80_02480"
     CDS             <1..907
                     /locus_tag="VFB80_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HZN32648.1"
                     /translation="GNWLDFRRFESHNSVDRDRFPQFTSELREAMFEEPIRFFLDNVQ
                     HDRSVLDSLYGKHTFVNPILAEHYGIDGVRGGADDWVRIDDATSYGRGGLLPMAVFLT
                     QNAPGLRTSPVKRGYWVVRRLLGERIPPPPPNVPELPADEAKLGELTLRETLARHRDH
                     ASCAGCHQRFDSIGLAFEGFGPVGERRLKDLGGRPIDTRASFPPVARHSLGETRDGVV
                     GYEGSGVEGLQHYLRTRREQEFVDNLCRKLLSYALGRSLLPSDDALVDTMRRRLAADN
                     HRFGVLVESIVTSRQFLTKRGNTDE"
     gene            900..2237
                     /locus_tag="VFB80_02485"
     CDS             900..2237
                     /locus_tag="VFB80_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN32649.1"
                     /translation="MSSRTLKNWHLSRRTILRGAGVAMALPWLESLPVFGAEPAADGA
                     PAVPRRFAALFMACGINSDHWWAKGSGEAMELGTTLEPLAPLKSKLNVISGLFNKAAT
                     GVGIHPGQTGNILSGAALQKGAVLRGGITMDQVLANAIGHQTVQPSLVLGCEQPITGY
                     HETNFSMAYSSHISWQNETSPVPMEVYPALAFDSLLENRGSRRNKSVLDRVMGEAASL
                     SRQVSAADKAKLEEYLQSVREVEQRVGRTRAGQIKAENRAADKSRPLVAMSRPDNGLP
                     EDIREHMRLMCDIVALAFQTDKTRVATLLLCRDISGLFYPFLDARKAHHSASHDDLSD
                     EYERISRYYVSQLAYLAQRLDAMPEAGGTVLDHSCLIFLSNMWSGSKHDSTKLPLVQV
                     GGLGGTIETGRVLDYRDKNDDDRKLCSLYLSVMDRMGCPLPRFGDAESRLAGL"
     gene            2250..3110
                     /locus_tag="VFB80_02490"
     CDS             2250..3110
                     /locus_tag="VFB80_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN32650.1"
                     /translation="MQNSEGRSSVSGASVPRFLYGTAWKEDRTRKLTELALAQGFRGI
                     DTANQRRHYHEAAVGEAIRAAMERGEVTRGELFLQTKFTFRGGQDHRLPYDPATAIAA
                     QVEQSFASSLAHLATDYIDSYVLHGPTQRSGLAAADWEAWRAMEALHAGGRAKLLGTS
                     NVSLEQLTRLCDQARVQPTFVQNRCYASRLWDRPVREFCTANGIVYQGFSLLTANREV
                     LAHPFVAEIARSYGRTAAQIVFRFATQIGMLPLTGTSDAHHMREDLAIFDFQLAPDEI
                     KRIETLAAGS"
     gene            complement(3118..4572)
                     /locus_tag="VFB80_02495"
     CDS             complement(3118..4572)
                     /locus_tag="VFB80_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32651.1"
                     /translation="MSIERLCLLTRRELLRRSGTGLGMVALAGVLSDAGVLRAATPPA
                     AGEEGYKNPLAERHPHFAPKAKRMIHLFMNGGPSHVDTFDPKPSLTKYAGQALPMPNL
                     ATERKTGAAFASPFQFQKYGESGTDVSELFHHTAQHIDDIAVIRSMHADVPNHEPSLM
                     LMNCGESRLVRPSLGSWLTYGLGTENLNLPGFIAMVPGGYPIVETQNWQSAFLPGVYQ
                     GTYVNTQFAEIEKLVENVRNHWLPAEAQRRQLDALAALNQHHKQQRGFDPALDARIHS
                     FELAYRMQREATEAFDVSREPQHVLDAYGEGTQARQILIARRLLERGVRFVQVWHGQG
                     QPWDNHDDIAVNHKKLAGQCDKAIGALIADLKRLGLFEETLILWGGEFGRTPTVELPQ
                     AGANAGKINGRDHNHWGFTCWLAGGGVKGGQTYGATDEFGFKAVEKPVHVHDLHATIL
                     KLMGFDHERLTYRYAGRDFRLTDVHGRVIPELIA"
     gene            complement(4562..>5397)
                     /locus_tag="VFB80_02500"
     CDS             complement(4562..>5397)
                     /locus_tag="VFB80_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN32652.1"
                     /translation="PFRDGSGRLELARAITAPANPLTRRVIANRLWMHHFGEPLALSP
                     SDFGIRSEPPSHPELLDELATRLLDADWSLKALHREIVNSAAYRQVSGDRPECRATDP
                     ENRLLWRMNRRRLELEAVRDTLLASASQLDTAMHGRPVELTKSPFPHRRAVYGFIDRQ
                     DLPNLFRVFDIASPDQSSPRRPRTTVPQQALFLMNSPFVIEQAQALSARPEIAAAADS
                     SAQVAALYRLVLHRPPDSEELAIGRDFLIAAQAQPDTKLAPLAQYAQLLLLTNEVMYV
                     D"
BASE COUNT          890 a         1750 c         1845 g          912 t
ORIGIN      
        1 cggcaactgg ctcgacttcc gccgcttcga gtcgcacaac agcgtcgatc gcgaccgctt
       61 cccgcaattc acgagcgagc tgcgggaggc gatgttcgaa gagccgatcc gcttcttctt
      121 ggacaacgtc cagcacgatc gctcggtgct cgactctctg tacggcaagc acacgttcgt
      181 caatccgatt ctcgccgagc actacggcat cgacggtgtt cgcggcggag cggacgactg
      241 ggtacggatt gatgatgcga cgagctacgg tcgcgggggc ctcttgccaa tggccgtctt
      301 cctgacgcag aacgcgccag gtctgcgcac cagcccggtc aagcgcggct actgggtcgt
      361 ccgccgcctg ttgggcgagc ggattccgcc gccgccgcca aacgtgccgg aattgccggc
      421 cgatgaggcc aagctcggcg agctgacgct ccgcgagacg ctcgccaggc atcgcgacca
      481 cgccagctgc gccggctgcc accagcggtt cgactcgatc ggcctggcct tcgaaggctt
      541 tggcccggtc ggcgagcggc ggctcaagga cctgggcggc cggccgatcg acacgcgagc
      601 cagctttcct cccgtagccc gccattccct gggggaaacg cgcgacggag tcgtgggcta
      661 cgaaggatcc ggcgtcgaag gcttgcagca ttacctccgc acgcggcgcg agcaggagtt
      721 tgttgataac ctttgccgca agctgttatc ctacgcgttg ggccgatcgc tcttgccttc
      781 cgacgacgct ttggtcgata ccatgcgtcg ccggctcgcc gcggataatc atcgcttcgg
      841 cgttttggtc gagtcgatcg tcaccagccg gcagtttttg accaaacggg gcaacaccga
      901 tgagtagccg cacgctcaaa aactggcacc tgtcccgccg cacgatcctc cgcggggccg
      961 gcgtcgccat ggcgctcccc tggctggaat cgctgccggt attcggcgcg gagccggcgg
     1021 ccgacggcgc cccggcggtt ccccggcggt ttgccgcgct gttcatggcc tgcggcatca
     1081 actccgacca ctggtgggcc aagggctctg gcgaggccat ggaactgggc acgacgctgg
     1141 agccgctcgc cccgctgaag agcaagctca acgtgatcag cggcctcttt aacaaggcgg
     1201 ccaccggcgt cggcattcac ccgggccaga cgggcaacat tctctccggc gcggccctgc
     1261 agaaaggcgc ggtgctccgc ggcggcatca ccatggacca ggtgctggcc aacgccatcg
     1321 ggcaccagac cgttcagccc agtctcgtcc tcggctgcga gcagccgatc accggctatc
     1381 acgagacgaa tttctcgatg gcctacagct cgcacatctc ctggcagaac gagacgtcgc
     1441 ccgtgccgat ggaagtgtac cccgcgctgg ccttcgacag cctgctggag aaccgtggca
     1501 gtcggcgaaa caagagcgtg ctcgaccgcg tcatggggga ggccgccagc cttagccggc
     1561 aggtgagcgc cgccgacaag gccaagctcg aggagtacct gcagagcgtc cgcgaagtcg
     1621 agcagcgcgt gggccgcacc cgcgcgggcc aaatcaaagc ggagaatcgc gctgcggaca
     1681 agagccggcc gctagttgcc atgtcgcggc ccgacaacgg tctgccggag gacatccgcg
     1741 agcacatgcg gctgatgtgt gacatcgtgg cgctggcgtt tcagaccgac aagacccgcg
     1801 tggccacgct gctcttgtgc cgcgacattt ccgggctgtt ctatccgttc ctggatgccc
     1861 gcaaggccca ccactcggcc tcgcacgacg acctctccga cgagtacgag cggatttcgc
     1921 gctattatgt cagccagctc gcgtaccttg ctcagcggct ggacgcgatg ccagaggcgg
     1981 gcggcacggt actcgaccac tcgtgcctga tcttcctgtc gaacatgtgg tcgggcagca
     2041 agcacgacag cacgaagctg ccgctcgtgc aggtcggcgg cctcggcggc acgatcgaaa
     2101 ccggccgcgt gctcgactat cgcgacaaga acgacgacga ccggaagctg tgcagcctgt
     2161 atctctcggt gatggaccgc atgggctgcc cgctgccgcg gttcggcgac gccgagagcc
     2221 ggttggccgg gctgtgagcg gaatgcagaa tgcagaattc agaaggacgg agctccgttt
     2281 ccggcgcaag tgttccgcgg ttcctgtatg gcacggcctg gaaggaagac cgcacgcgga
     2341 aattgactga gctggcgctt gcgcagggct tccgcggaat cgacacggcc aaccagcggc
     2401 ggcactatca cgaggccgcg gtcggcgagg cgatccgcgc ggcgatggag cgcggcgaag
     2461 tgacgcgcgg tgagctgttc ctgcagacca agttcacgtt tcgcggcggg caggaccacc
     2521 gtttgccgta cgaccccgcg acggcgatcg ccgcgcaggt cgagcagtcg ttcgccagct
     2581 cgttggcgca tctggcgacc gactacatcg actcgtacgt cctgcacggg cccacccagc
     2641 ggagcggcct ggcggctgcc gactgggaag cctggcgggc gatggaggcg ctgcatgccg
     2701 gcggccgcgc caagctgcta ggcacgagca acgtcagcct ggagcagctg acgcggctgt
     2761 gcgaccaggc ccgcgtgcag cccacgttcg tgcagaaccg ttgctacgcg tcgcggttgt
     2821 gggaccggcc agtccgcgag ttctgcactg ccaatggaat cgtctatcaa ggcttttcgc
     2881 tgctcaccgc caaccgcgaa gtgctggcgc atcccttcgt ggcggagatc gcgcgaagtt
     2941 atggccgcac tgcggcgcag atcgtgttcc gcttcgccac gcagatcggc atgttgccgc
     3001 tcaccggcac cagcgacgcc caccacatgc gggaggacct ggcgatcttc gacttccagc
     3061 tcgcgcccga tgaaatcaag cggatcgaga cgctggccgc cggttcgtag ccggcgttca
     3121 ggcgatgagc tctgggataa cccggccgtg cacgtcggtc aggcggaagt cgcggccggc
     3181 gtagcggtag gtcagacgct catggtcgaa gcccatgagc ttcaggatcg tggcgtgcag
     3241 atcgtggaca tggaccggtt tttcgaccgc tttgaaaccg aattcgtcgg tcgcgccgta
     3301 ggtctggcca cctttgacgc cgccgcccgc gagccagcag gtgaagcccc agtggttatg
     3361 gtcgcggccg ttgatcttac ccgcgttcgc tccggcctgc ggcaactcga cggtcggcgt
     3421 gcggccgaac tcgccgcccc agaggatcag cgtttcctcg aacaggccca gccgcttgag
     3481 gtcggcgatg agggccccga ttgccttgtc gcactggccg gcgagcttct tgtgattgac
     3541 ggcgatgtcg tcgtggttgt cccagggctg gccctgcccg tgccagacct ggacgaaccg
     3601 cacgccccgt tccagcaggc ggcgggcgat gaggatttgc cgggcttgcg tcccctcgcc
     3661 gtaggcgtcg agcacgtgct ggggctcgcg gctgacgtcg aaggcttcgg tggcctcgcg
     3721 ctgcatgcgg taagccagct cgaaactgtg gatccgcgcg tcgagggccg ggtcgaagcc
     3781 gcgctgctgc ttatggtgct ggttgagagc ggcgagagcg tcgagctgcc gccgctgggc
     3841 ttcggccggc agccagtggt tgcggacgtt ttcgacgagc ttctcgatct cggcgaactg
     3901 cgtgttgacg tacgtgccct gatagacgcc cggcaggaac gccgactgcc agttttgcgt
     3961 ctcgacgatc ggatatccgc cggggaccat ggcgatgaac ccgggcaggt tgaggttctc
     4021 cgtgcccagg ccgtaagtca gccaggagcc gaggctcggc cgcacgagcc gcgactcgcc
     4081 gcagttcatg agcatcagcg agggctcgtg gttgggaacg tcggcatgca tcgaacggat
     4141 gacggcgatg tcgtcgatgt gctgagccgt gtggtgaaac agctcgctga cgtcggtgcc
     4201 gctctcgccg tacttctgga actggaaagg cgaggcgaat gccgcacccg tcttccgctc
     4261 ggtggccagg ttcggcatcg gcagcgcctg gccggcgtat ttcgtcaggc tcggcttggg
     4321 atcgaaggta tcgacgtgcg aagggccccc gttcatgaac aggtggatca tccgcttcgc
     4381 cttcggcgcg aagtgcggat gccgctcggc cagcgggttc ttgtaaccct cctcgcccgc
     4441 cgcgggcggc gtcgccgccc gcagcactcc ggcgtccgac aacacgcctg ccagcgcgac
     4501 catacccagg cctgtacccg agcggcggag cagctcgcgg cgggtgagca agcagagtcg
     4561 ctcaatcgac atacatcacc tcattcgtga gcagcagcaa ttgagcgtat tgggccagcg
     4621 gcgcgagctt tgtgtccggc tgagcttggg ccgcgatcag aaagtcgcgg ccgatggcga
     4681 gttcctcgct gtcgggcggg cggtggagga ccaggcgata gagcgcggcg acctgggcgg
     4741 agctgtcggc ggccgcggcg atttcgggac gggcggagag ggcctgcgcc tgctcgatga
     4801 caaagggcga gttcatcagg aacagcgcct gctgcgggac agtcgtccgc ggtcggcggg
     4861 ggctgctttg atcgggactg gcgatgtcga aaacgcggaa caggttgggc aagtcctggc
     4921 ggtcgatgaa gccatagacg gcgcggcggt gcggaaacgg cgacttcgtc agctcgacgg
     4981 ggcggccatg catcgccgtg tcgagctggc tcgccgatgc cagcagcgta tcgcgaacag
     5041 cctccagctc caggcggcga cggttcattc gccacagcag gcggttttcc ggatcggtcg
     5101 cgcggcactc cgggcggtcg ccgctcacct gccggtaggc agccgaattg acgatctcgc
     5161 gatgcagggc cttgagcgac caatcggcgt ccaagagccg cgtcgctagt tcatcgagca
     5221 gttcaggatg gctgggcggc tcgctgcgaa tgccgaaatc gctgggcgaa agcgccagcg
     5281 gctccccgaa atgatgcatc cacagccgat tggcgatgac ccgccgcgtc agcggattgg
     5341 ccggtgcagt gatcgcgcgg gccagttcca atcggccgct gccgtcgcgg aacggcg
//
LOCUS       DASRJB010000086         7001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1152284, whole genome shotgun sequence.
ACCESSION   DASRJB010000086 DASRJB010000000
VERSION     DASRJB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152284"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFB80_02505"
     CDS             <1..483
                     /locus_tag="VFB80_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZN32653.1"
                     /translation="FYVVALLFIIFDVEVAFFFPWAAVFGKSTNLMDPQFQVVQRAEN
                     GAVTLTKPAADLYKELGVRNPTAPQEVPPSLAAEVTVADDLARADQVIHLGARQLAWV
                     TVVDIAAFFAVLLIGFAYVWRRGDLDWVRAVGQAHAAAPPALPGSDDPLEQEAAALSA
                     "
     gene            523..1053
                     /locus_tag="VFB80_02510"
     CDS             523..1053
                     /locus_tag="VFB80_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZN32654.1"
                     /translation="MAGPTLLNRLQQKFGDKIAGANNEAIDPWIEVKPEGLVEVCRYL
                     KTEPDLSFELLNCISGVDYFEPDPKKAAKAAFQPHTEVVYHLSSVTKKHSLVLKVILP
                     RWKNDEEGELPEVPTVSGVWSGANWHEREVFDLSGVRFTGHPDLRRILCPEDWEGYPL
                     RKDYEMPLEYHGIRGR"
     gene            1137..1547
                     /locus_tag="VFB80_02515"
     CDS             1137..1547
                     /locus_tag="VFB80_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZN32655.1"
                     /translation="MARSNFENLRVYRLSEEIADAIWDVANGWRPFARDTVGKQLVRA
                     ADSIGANIAEGDGRGSFQEHRRFIRIARGSLNETKHWLRRAFRRKLLSQRQIEQLRPL
                     LDELGPRLNAYLKSIGRSRPAASTRSSPPLTTDN"
     gene            1570..2775
                     /locus_tag="VFB80_02520"
     CDS             1570..2775
                     /locus_tag="VFB80_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZN32656.1"
                     /translation="MPLTLDDPRVVEFDVRTDEMLVNMGPQHPSTHGVLRLVLRTDGE
                     IVSETQPHIGYLHRSAEKIGENLTPRQWIPYTDRMDYLAGMSMNLGWSLVVEKLLNYQ
                     IPEKARHLRVIIAEMQRIASHLVGMGAYGLDLGTFSPFLYAFREREKILDLFEEACGA
                     RLTYSYLTPGGATADLPRGWLQKCSAFLDQFEPVIQEYHALLTTNAIFVKRTANIGLL
                     SPQMALDYGCTGPVLRGSGIDYDLRRDGEERYTEMYDGYAFEIIVQRNGHYPKDHDYP
                     RVPKETVLGDCWHRFYVRMLEVVQAVDLVRQAIDRYSTAQGPWGEPIKLTQKLPKGEA
                     YLETEAPRGQMGFYIVSDGSSIPWRARARSSCFCNLSVTHELCRGCLIADVPAIVGSL
                     DIVMGEIDR"
     gene            2851..3300
                     /locus_tag="VFB80_02525"
     CDS             2851..3300
                     /locus_tag="VFB80_02525"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32657.1"
                     /translation="MEAVILIGLQGAGKSTFYRQRFAPSHAHVSLDVLKTRHRERTLL
                     AECIAQRRPLVVDNTNPARADRAAYIALAMQAGYRALGYYFHSRVENCHRRNLQRPAE
                     QQIPLAGLLGTAGRLELPAYDEGFDELHYVRITSEGAFAVQEWRDEV"
     gene            3290..4066
                     /locus_tag="VFB80_02530"
     CDS             3290..4066
                     /locus_tag="VFB80_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HZN32658.1"
                     /translation="MKFDELDARLRVFETAADQWVLPRLFLVARLDGRGFTRLTKETH
                     AFAAPFDERFRDLMLATAEHLMTASGFRFLYGYTESDEISLLFAPGEDKFGRKHRKLL
                     SILSGEASARFSLLLGSVAVFDCRLSQLPTADLVVDYFRWRSEDAHRNALNAHCYCLL
                     RRQGANAAAATARLERLSTAAKNELLFQEGVNFNNLPLWQRRGVGLAWETYERPATNP
                     RTGAATVAVRRRLRPLLELPMKEAYSEFLRCLLADSAVVC"
     gene            4112..4879
                     /locus_tag="VFB80_02535"
     CDS             4112..4879
                     /locus_tag="VFB80_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN32659.1"
                     /translation="MWDADQYLKFADERSRPFFDLLAQVRHEQPKLVVDLGCGPGHLT
                     RAAADRWPRGLFIGVDNSANMLAQARPLTVAGHLEFELADIESWTPPQPVDVILSNAA
                     LQWVRHHDLLLPRLAGMLAPGGTLAVQVPDFFHMPAHAAIDAAASEPEWADKLREAGV
                     PRDSVQPLDWYVHRLIELGFAVNAWQTTYYHVLRGENAVLEWFKGSALRPFLALLDDE
                     SAAEFLGEVNRRLKAAYPITNGVTLLPFVRLFFVATR"
     gene            complement(4928..>7001)
                     /locus_tag="VFB80_02540"
     CDS             complement(4928..>7001)
                     /locus_tag="VFB80_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN32660.1"
                     /translation="CILAQPIRAQTAYPMLMSVRPVAVQVGQTAEVEVKSRYSMHGAY
                     QVLVSGERVSGEVILPEVKQDDPAKKPPADKPLEKIKVTFTAAADALPGVRDVRLATP
                     QGVSTVGQLVVVRDPVIVESGNNDTPPQAQQIALPAAICGAIEKNEDVDYFRFQAEAG
                     ASVAFLVRCARLEDRIHDLQTHADPIITLRTAAGATLAASDNTSYHADPVLAYKFEQA
                     GEYLLEIRDVRYQGNQYWEYVIEAGSRPLVEVTWPLAVRPGEEKQLQPVGLLIAADAN
                     LAFAVPADARPGLADFQLPVGGELTNPIPLLVSDLPLVSESDAPNDAPDAAVAVNVPA
                     GINGRVERDGDVDCFAFDAKKGEAFSLEVVARRARSALDAHLRVLDPKGKQLALNDDL
                     RLGKRGSADSWIENWTAPADGRYVAEVRDVNLRGGPDFPYFLKITRSEPYFELFLDTD
                     KTQVCPGGCGAIFVRIERKNGFTGEVQLAMDGLPPGITASCGRILEGKGQDGCIVLEA
                     AADAKLAVANVVVTGKGIHQADGKELELTADAVPYQETYQPGGGRGHWPVAAHTVAIS
                     EPADIRGITLSTYDITLQPGESQKIEITIDRGPGFAANVTLDMLMRHLNSTYANTLPP
                     GVTLNDKDAKSLLAGSATQGFLTLTAAKDAAPVEKQQAVVVAHVALNFVMKWTYASRP
                     VTITVARP"
BASE COUNT         1181 a         2290 c         2280 g         1250 t
ORIGIN      
        1 ttctacgtgg tggccctgct gttcatcatc ttcgacgtcg aggtcgcgtt cttctttccg
       61 tgggccgcgg tcttcggcaa atcgacgaac ctgatggatc cgcagttcca ggtggtccag
      121 cgggccgaaa atggcgcggt caccctgacg aagccggcag ccgacttgta caaggaactg
      181 ggcgtccgca atccgaccgc gccgcaagag gtgcccccct cgctcgcggc ggaagtgacg
      241 gtggccgacg acctggcccg ggccgaccag gtgatacatc tgggagcgcg gcagctggcc
      301 tgggtgacgg tggtcgatat cgcggcgttt ttcgccgtgc tgctcatcgg ctttgcctac
      361 gtctggcggc gcggcgacct cgactgggtc cgggccgtcg gccaggccca cgccgccgca
      421 ccgcccgcgc tgcccggcag cgacgacccg ctggagcagg aagccgcggc gctctccgcg
      481 taagttttcg acgtttcttc gacattcgac attgcctcga tcatggctgg cccaaccctc
      541 ctcaaccgtc tccagcagaa gttcggcgac aagatcgccg gagcaaacaa cgaggccatc
      601 gatccctgga tcgaagtcaa gccggaaggg ctcgtggaag tctgccggta tctgaagacc
      661 gagccagact tgagcttcga gctgctgaac tgcatttcgg gcgtcgatta cttcgagccc
      721 gatccgaaga aggccgccaa ggctgcgttt cagccgcaca cggaggtcgt ctatcacctg
      781 tccagcgtga ccaagaagca ctcgctggtg ctgaaggtga tcctgccgcg gtggaagaac
      841 gacgaggagg gcgaattgcc cgaagtgccc accgtcagcg gcgtctggtc cggcgcgaac
      901 tggcacgagc gtgaagtgtt cgacctttcc ggcgtccgct tcaccggcca ccccgacctg
      961 cgaagaattc tctgtcccga agactgggaa ggctatccgc tgcggaaaga ctacgagatg
     1021 ccgctggagt atcacgggat acgcggcagg taggctattg tcagttgtcc gtggtcagtg
     1081 gtcagttgga gggacgcgga gggtgaatct gaatctgctt ctgacgaagg cacatcgtgg
     1141 cacgctccaa ctttgaaaac ttgcgcgtct atcggttgtc cgaggagatc gcagacgcca
     1201 tttgggatgt ggctaacggc tggagacctt tcgcgcggga taccgtcgga aagcaactag
     1261 ttcgcgcggc tgacagcatt ggagcgaaca tagccgaagg cgacggtcgc ggctcctttc
     1321 aagaacatcg gcgattcatt cgcatcgccc gcggctcgtt gaatgaaaca aagcactggc
     1381 tgcgccgagc gtttcgaaga aaactgttat cgcagcggca gatcgagcaa ctcaggccgt
     1441 tgctcgacga gcttggcccg cgcctgaatg cctatttgaa gtcgataggc cgatcccggc
     1501 cagccgcgtc aacgcgatcg tcgcccccac tgacaactga caactgacca ctgaatccgc
     1561 cccagagcca tgcccctcac gttagacgat ccgagagtag tcgaattcga cgtccgcacc
     1621 gacgagatgc tcgtcaacat gggaccgcag catcccagca cgcacggcgt gctgcggctg
     1681 gtgctgcgga ccgacggcga gatcgtctcc gagacgcagc cgcacatcgg ctacctgcac
     1741 cgcagcgccg agaagatcgg cgagaatttg acgccgcggc agtggattcc ctacaccgac
     1801 cggatggact acctggccgg gatgagcatg aatctcggct ggtcgctggt ggtcgagaag
     1861 ctgctcaact atcagattcc cgaaaaagcc cggcacctgc gggtgatcat cgccgagatg
     1921 cagcggatcg ccagccacct ggtcggcatg ggggcctacg gcctggacct gggcacgttc
     1981 agcccgtttc tgtacgcctt ccgcgaacgg gagaagatcc tcgacctgtt cgaagaggcc
     2041 tgtggcgccc ggctgaccta cagctatctg acgcccggcg gcgcgacggc cgatctgccc
     2101 cgcggctggc tgcagaagtg ctcggcgttt ctcgaccagt tcgagccggt gatccaggaa
     2161 tatcacgccc tgctcacgac caacgcgatc ttcgtcaagc ggaccgcgaa catcggcctg
     2221 ctttcgccgc agatggctct cgactacggc tgcacgggcc cggtcctccg cggcagcggc
     2281 atcgactacg acctgcgccg cgacggcgaa gagcggtaca cggagatgta cgacggctac
     2341 gccttcgaga tcatcgtgca gcgcaacgga cactatccca aggaccacga ttacccgcgg
     2401 gtgccgaagg aaacggtgct gggcgactgc tggcaccggt tctacgtgcg gatgctggag
     2461 gtcgtgcagg cggtggatct ggtgcggcag gcgatcgacc gctacagcac ggcgcagggc
     2521 ccctggggcg agccgatcaa gctcactcag aagctgccca agggcgaggc ctacctggag
     2581 accgaagctc cgcgcggcca gatgggcttc tacatcgtca gcgacggcag ctcgattccc
     2641 tggcgggccc gcgcccgcag ctcctgcttc tgcaacctct cggtcaccca cgagctctgc
     2701 cgcggctgcc tcatcgccga cgtgcccgcc atcgtcggct cgctcgacat tgtgatgggc
     2761 gagatcgacc gctagccgta gggcgggcct ccggcctgtc ctacaaaagc cgctgcgaac
     2821 caacctttgc agccggctgt cctgccgtcc gtggaagccg tcatcctgat cggcctgcaa
     2881 ggggcgggca aatcgacgtt ctatcgccag cgtttcgcgc cgtcgcacgc tcacgtcagt
     2941 ctggacgtgc tgaagacgcg gcatcgcgag cgaacgctgc tggccgagtg catcgcccag
     3001 agacggccgc tggtcgtcga taacacgaac cccgcccgtg cggatcgggc ggcttacatc
     3061 gccctggcga tgcaggccgg ctatcgcgct cttggctact acttccattc gcgcgtggag
     3121 aattgccatc gccgtaatct gcagcggccg gccgagcagc agattccgct ggccggcctg
     3181 ctgggaacgg ccgggcggct cgaactgccc gcctacgacg agggcttcga cgagttgcac
     3241 tacgtcagga tcaccagcga gggggccttt gcagtccagg agtggcgcga tgaagtttga
     3301 cgagctggat gcccgcctgc gcgtgtttga gaccgccgcc gaccagtggg tgctgccgag
     3361 actgttcctg gtggcccgac tcgatggccg cggcttcacg cggctgacca aagaaacgca
     3421 cgcgtttgcc gctccgttcg acgagcggtt tcgcgacctg atgctggcga ccgcggagca
     3481 cctgatgacg gcctccggct tccgcttcct ctacggctac accgagagcg acgaaatttc
     3541 gctgctcttc gcgccgggcg aagacaaatt cggccgcaag caccgcaaat tgctgtcgat
     3601 cctctcgggc gaagccagtg ccaggttctc gctgctgctg ggatcggtcg ccgtcttcga
     3661 ttgccggctt tcccagttgc caaccgcgga cctggtcgtg gactacttcc gctggcgcag
     3721 cgaagacgcc caccgcaacg cactgaacgc ccactgttat tgcctcctgc gccggcaagg
     3781 cgccaatgcc gccgcggcca cggcccggct ggagcggctc agcactgcgg cgaaaaacga
     3841 actgctgttt caagagggcg tcaacttcaa caacctcccg ctgtggcagc gccgcggcgt
     3901 cggcctggct tgggaaacat acgagcggcc cgccacgaac ccgcgcaccg gcgcggccac
     3961 cgtcgccgtg cgccgccgtt tgcggccatt gctcgagctg ccgatgaagg aagcctattc
     4021 ggagtttctc cgctgcctgc ttgcggattc agcagtcgtt tgctgaactc catgccatcg
     4081 tcagaactgt ttcaacggtc gaggactggg gatgtgggac gccgatcaat acctgaaatt
     4141 cgccgacgag cgctcgcggc cgttctttga tttgttggct caagttcggc acgagcagcc
     4201 gaagctggtc gtcgatctgg gctgcgggcc ggggcatttg acgcgcgcgg ctgcggaccg
     4261 ctggccgcgg gggctcttca tcggcgtcga taactcggcg aatatgctgg cccaggcgcg
     4321 gccgctgacc gtggccggac acctggagtt cgagctggcg gatattgagt cctggacgcc
     4381 gccgcagccg gtcgatgtga tcctcagcaa cgcggcgctg cagtgggtcc ggcaccacga
     4441 cctgctgctg ccgcggctgg ccggaatgct cgcgccgggc ggcacgctgg cggtgcaagt
     4501 tcccgacttc ttccacatgc cggcccacgc cgcgatcgac gccgcggcca gcgagccgga
     4561 gtgggcggac aagctacgcg aggccggcgt gccgcgcgat tcggtgcagc cgctcgactg
     4621 gtacgtccac cggctgatcg agctgggctt tgcggtcaac gcctggcaga cgacttacta
     4681 tcacgtgctg cgcggcgaga acgcggtgct ggagtggttc aagggctcgg cgctgcgacc
     4741 gttcttggcg ctcttggacg acgagtcggc ggcagaattc ctgggcgagg tgaatcgccg
     4801 gctgaaggct gcgtatccga tcacgaacgg cgtcacgctg ctgccgtttg tgcggctgtt
     4861 ctttgtggcg acgcggtgac tcgtagccga cgctgccagc gtcggcaatc gatgctggca
     4921 gcatcggcta cggcctggcc acggtgatcg tcaccggcct gctggcgtag gtccacttca
     4981 tcacgaagtt cagcgccacg tgggccacga cgacggcctg ttgcttctcc accggagcgg
     5041 cgtctttggc ggcggtgagc gtcaggaagc cttgcgtcgc gctgccggcc agcagcgact
     5101 tggcgtcctt gtcgttcagg gtcacgcccg gcggcagcgt attggcatag gtcgagttga
     5161 ggtgccgcat cagcatgtcc agcgtgacgt tggcggcgaa gccggggccg cggtcgatgg
     5221 tgatttcgat cttctgcgac tcgccgggct ggagcgtgat gtcataagtg ctgagcgtga
     5281 tgccgcggat atcggccggc tcgctgatcg cgaccgtgtg agccgcgacc ggccaatggc
     5341 cgcgtccgcc gccgggctgg taggtctcct gatagggaac ggcgtcggcc gtcagctcca
     5401 gctccttgcc gtccgcctga tgaatgcctt tgccagtcac gacgacattc gccacggcca
     5461 gcttcgcgtc ggccgcggcc tccagcacga tgcagccgtc ctggcctttg ccttcgagaa
     5521 tccgcccgca actcgcggtg attcccggcg gcagcccgtc catggcaagc tgcacctcgc
     5581 ccgtaaaccc gttcttgcgc tcgatgcgga cgaagatcgc gccgcagccg ccagggcaca
     5641 cctgcgtctt gtcggtgtcg agaaacagct cgaagtaggg ctcgctgcga gtgatcttca
     5701 ggaaataggg gaaatcgggt ccgccgcgga ggttcacatc gcggacttcg gcgacgtagc
     5761 ggccatccgc cggcgcggtc cagttctcga tccaggaatc ggccgagccg cgcttgccca
     5821 agcgcaggtc gtcgttcagg gccagttgct tgcccttggg gtcgagcacg cgcaggtggg
     5881 catcgagcgc cgagcgggcc cggcgggcga cgacttccag cgaaaacgct tcgcccttct
     5941 tggcgtcgaa ggcgaagcaa tcgacgtctc catcgcgctc gacgcggcca ttgattcccg
     6001 ccggcacgtt gacggcaacg gcggcatccg gcgcgtcgtt cggagcatcg gactcgctca
     6061 ccagcggcaa gtcggacacg aggagcggga ttggatttgt cagctcgccg ccgacgggca
     6121 actgaaagtc cgccaggccg ggccgcgcat cggcaggcac agcgaacgcc aaattcgcgt
     6181 cagccgcaat gagcaaaccc accggctgca gctgcttctc ttcgcccggc cgcactgcca
     6241 gcggccaggt cacctccacc agcggccgac tgccggcctc gatcacgtat tcccagtact
     6301 gattgccctg ataccgcacg tcgcggatct ccagcaggta ctcgccggcc tgctcgaact
     6361 tgtaggccag caccggatcg gcgtggtagg aggtgttgtc gctggcggcc agcgtcgcgc
     6421 cggcggcggt gcggagcgtg atgatcggat cggcgtgggt ctgcaggtcg tggatgcggt
     6481 cctccaggcg ggcgcagcgg acgaggaagg ccacggaagc tcccgcctcc gcctggaacc
     6541 tgaaataatc gacgtcttcg ttcttctcga tcgcgccgca gatcgccgcc ggcagggcga
     6601 tttgctgggc ctgcggcggc gtgtcgttgt ttccgctctc gacgatcacc gggtcgcgaa
     6661 cgacgaccag ctgccccacc gtgctcaccc cctgcggcgt cgccagccgc acgtcgcgga
     6721 ctcccggtag ggcgtcggcc gccgccgtga acgtgacttt gattttctcg agcggcttat
     6781 cggcgggcgg cttctttgcc ggatcgtcct gcttgacctc aggtaggatg acctcgccgc
     6841 tgacgcgttc gccgctcacc agcacctggt aggccccatg catcgaatac cgcgacttga
     6901 cctccacctc cgcggtctgc ccgacctgca ccgcaaccgg acgcacgctc atcagcatgg
     6961 gataggcggt ttgggcgcga atcggctgcg cgaggatgca a
//
LOCUS       DASRJB010000087         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3410673, whole genome shotgun sequence.
ACCESSION   DASRJB010000087 DASRJB010000000
VERSION     DASRJB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410673"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VFB80_02545"
     CDS             complement(<1..1237)
                     /locus_tag="VFB80_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HZN32661.1"
                     /translation="MRYLGGLAACSLFVLSAVGQAHEGGAHSAAAVIRSMKSGPWSAA
                     ETWEGGRVPAAGARVLIRPGHQVTYDMKSDAAIRTVQVGGTLAFARDRDTLLNVGLLR
                     VSPGESFSEEGFDCDAHLPAKTAEADRPALLVGTAAEPIHAGKSAVIRLTYHDGMSRD
                     SCPAIVCCQGRMEFHGVPIAHTWTKLVAPAAAGDTAIATLDPVSDWRVGNRIILTATT
                     RQNKLKKTFQPTLRGGAVQTEERLIAAVDGNQLTLDKPLAFAHTAEGDYRADVANLSR
                     NVVVESADPAAARGHTMYHRDSTGSIGYAEFRHLGKEGVLGRYSIHFHLVGDTMRGAS
                     VVGASVWDSGNRWITIHGTNYLVVRDCVGYQSVGHGFFLEDGTEAFNVLDHNLAVQAY
                     LGKPLPKQVLPYDKNDGSGF"
     gene            complement(1305..2072)
                     /locus_tag="VFB80_02550"
     CDS             complement(1305..2072)
                     /locus_tag="VFB80_02550"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZN32662.1"
                     /translation="MSDRFFANEPITGLRAALAGDEARHLVGVMRAKTGDEVVLFDGS
                     GAEFACRVLKVGKQQVELEIIERREVSRELSVAVTLAVALPKGERQKWLVEKATELGV
                     TRIVPLTTQRGVAQPTDAALARLRRTVIEASKQCGRNRLLEIGSPADALQFFASAPTA
                     ALRLLAEPGAAPLGEVAARSQEIVAAVGPEGGLTPHERSAALAAGWQPVSLGPAILRV
                     ETAAIAIAAWAATADRLNAKEQGGKDAKQDADKGRSL"
     gene            2211..>2754
                     /locus_tag="VFB80_02555"
     CDS             2211..>2754
                     /locus_tag="VFB80_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32663.1"
                     /translation="MYEAYWGFARSPFSARAVRESLAASPVHAEALARLDFLLSSRSP
                     LGLLLGECGSGKSAVLAVFAERATRAGALACRIAAAAAGEEHVLPALAEALGVAESGE
                     RLVLWRRLVDRLAELRFEGVSAAVLLDDLDRAAPTTLALVERLLAVVDAPLTIVASAR
                     PDAAPRIGRRLLEQVALRIEL"
BASE COUNT          392 a          899 c          985 g          478 t
ORIGIN      
        1 aaaacccgga gccgtcgttt ttgtcgtacg gcagcacctg cttgggcagc ggcttgccca
       61 ggtaggcctg cacggcgagg ttgtggtcga gcacgttgaa ggcctcggtg ccgtcctcga
      121 ggaagaagcc gtggccgacg ctttggtagc cgacgcagtc gcggacgacg aggtagttgg
      181 tgccgtgtat cgtgatccag cggttgccgc tgtcccagac cgaggccccg accacgctcg
      241 ctccccgcat cgtgtcgccg accaggtgga agtggatgct gtagcggccg agcacgccct
      301 ccttgccgag gtggcggaac tcggcgtagc cgatcgagcc ggtcgaatcg cggtgataca
      361 tcgtgtggcc gcgagcggcg gcgggatcgg ccgattcaac gaccacgttg cggctcaggt
      421 tcgccacgtc cgcccggtaa tcgccctcgg cggtatgggc aaaggccagc ggcttgtcga
      481 gcgtcagctg gttgccatcc actgccgcga tcagccgctc ttcggtttgc acagctccgc
      541 cgcggagcgt cggctggaag gttttcttga gcttgttctg ccgcgtcgtg gccgtgagga
      601 tgatccggtt gccgacccgc cagtcgctga cgggatcgag cgtggcgatc gccgtgtcgc
      661 ccgcggcggc gggtgcgacg agcttggtcc aggtgtgggc gattggcacg ccgtgaaact
      721 ccattcgccc ctggcagcag acgatggccg ggcaggagtc ccggctcatc ccgtcgtgat
      781 acgtcaggcg gatgacggcc gacttgccgg catgaatcgg ctcggcggcc gtgccgacca
      841 gcagcgccgg gcggtcggcc tcggccgtct ttgccggcag gtgggcgtcg cagtcgaaac
      901 cctcttcgct gaacgattcg ccgggcgaaa cccgcagcag gccgacattg agcagcgtgt
      961 cgcggtcgcg ggcaaacgcg agcgtcccgc cgacctgcac cgtgcggatc gccgcgtcgc
     1021 tcttcatgtc ataggtgacc tggtgtcccg ggcgaatcag cacccgcgcg ccggccgccg
     1081 ggaccctgcc gccttcccac gtctcggcag ccgaccaggg gccgctcttc atcgagcgga
     1141 tcacagcggc agcggaatgc gccccgcctt cgtgggcctg acccaccgcg gacaagacga
     1201 acaacgagca tgccgccaga ccaccgagat atcgcatcga cagacctttc tgaaacgcgg
     1261 cgcctattcg attaacgcaa aggcgcggag ggaaccaaga cagattatag gctgcgtcct
     1321 ttgtctgcat cttgctttgc gtctttgcct ccttgctcct ttgcgttaag gcggtcggct
     1381 gtcgccgccc aggccgctat ggcgatggca gcggtttcga cgcggaggat ggccggtccc
     1441 agcgagaccg gctgccagcc ggcagccagc gcggcggatc gttcgtgcgg cgtgaggccc
     1501 ccttctgggc cgacagcggc gacgatctcc tggctgcggg cagcgacttc accgagcggc
     1561 gcagcgcccg gctcggcgag caaccgcagg gcggcggtgg gagcagaagc aaagaactgc
     1621 agggcatccg ccgggctgcc gatttccagg agccggttgc ggccgcattg cttggaggct
     1681 tcgatcacgg ttcgccgcag ccgggcgagc gcggcgtcgg tcggctgggc gacgccccgc
     1741 tgggtggtca gcgggacgat tcgcgtgacg cccagctcgg ttgccttctc gacgagccat
     1801 ttttgccgct cccctttcgg cagtgccacg gcgagcgtaa cggccaccga cagctcgcgc
     1861 gagacttcgc ggcgctcgat gatctccagc tcgacttgct gcttgccgac cttcagcacg
     1921 cggcaggcga actccgcgcc gctgccgtcg aagagtacga cctcgtcgcc cgttttggcc
     1981 cgcatcacgc ccaccaggtg ccgggcctcg tcgccggcga gtgcggcgcg gaggccggtg
     2041 atgggctcat tggcgaagaa gcggtcggac atgtgcaggt ggtcggtcgg tcggtggtcg
     2101 gttcgtcggt gggtcgtacc caccgaccac cgacccgcct tcaagttcgg cggcggttct
     2161 gccgatgggt aacaagatac gctgaagtct gcgcggataa aggactggcc atgtacgagg
     2221 cttattgggg ctttgcccgt tcgccatttt ccgcaagggc ggtgcgcgag tcgctggcgg
     2281 ccagcccggt gcatgccgag gcgctggccc gactcgattt tctgctgagc agccgcagcc
     2341 cgctgggctt gctcttgggt gagtgcggca gcggcaagtc ggcggtgctg gcggtctttg
     2401 ccgaacgggc gacccgcgcc ggcgcgctcg cctgccgcat cgcggccgcg gcagccggcg
     2461 aagagcacgt gctgcccgcg ctggccgaag cgctgggggt cgccgagagc ggcgagcggc
     2521 tcgtgctgtg gcggcggctg gtcgatcgcc tggcggagct gcgtttcgag ggagtctcgg
     2581 cggcggtgct gctggacgat ctcgatcgcg ccgcgccgac gacgctggca ttggtcgagc
     2641 gattgctggc ggtggttgat gccccgctga ccatcgtggc gtccgcgcga ccggatgcag
     2701 cgccgaggat cggccgccgc ttgctcgagc aggtcgcact gcggatcgaa ctga
//
LOCUS       DASRJB010000088         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3693006, whole genome shotgun sequence.
ACCESSION   DASRJB010000088 DASRJB010000000
VERSION     DASRJB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693006"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="panB"
                     /locus_tag="VFB80_02560"
     CDS             complement(<1..604)
                     /gene="panB"
                     /locus_tag="VFB80_02560"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZN32664.1"
                     /translation="MAKRSSRVTVPEFIAFKTAGRRISMLTAYDYPTAALLDGAGIDA
                     ILVGDSMSMVVQGHDTTLPVTLDEMIYHAEMVGRAVERALVVVDLPFPTYHLGVHRAI
                     ESAGRILKEARCQAVKLEGGADQADVIRGLVSAGIPVMAHVGLRPQSVHQMGGYRVQR
                     DAQRLMADAAAAQEAGAFAIVVECIPASAAAEITRSLKIPT"
     gene            706..2433
                     /gene="lnt"
                     /locus_tag="VFB80_02565"
     CDS             706..2433
                     /gene="lnt"
                     /locus_tag="VFB80_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN32665.1"
                     /translation="MLKAAPRRSLARPSAAAPQEAEPAARWRPQFLTTLGCGAAGALL
                     LWAAFPPLNWPLLAWLAPVPWLWLIRLPALPGWRPYFMLWLAGSFHWLLVLQGIRLAH
                     PALYGGWIALSLYLAVYLPVFIGLTRVAVHRLKAPLVASAPVVWVGLELLRGHLVTGF
                     SNGLLAHTQAEIPLLIQVADLAGGYTLSFVLMFVAACLAMLIPVAGACGPRSGPQPAR
                     TWSVVWPPLLIAAVMLATLLYGNWRLAQKTAAGPTAKVALLQGALDTVFGEDRSAETF
                     EHYRQLTDQAVAGRADLDLVVWPESSFPAPEVLIEEPLAPLPNMSVSQLRSNADVAQR
                     QLRDLLAGEALRANANTNSGAAGTRLLVCGTSFVYGPAQPRMYNAALVIDPRGDMACR
                     YYKTHPVMFGEYIPFGEAFPWLYKIQPLGMGLSRGDGPRVFEAGGLRMSPNICFESTV
                     PHLVRGQLRELSRRGTPADVIVNVSNDGWFWGSAILDLHFRSNIFRTVENRRPLLVAA
                     NTGISAWIDSSGVVRQRGQKRTPQVLYAEIQADGRASPYSLIGDWPAWICAAACAGLA
                     LAGFRRRAE"
     gene            2688..3512
                     /locus_tag="VFB80_02570"
     CDS             2688..3512
                     /locus_tag="VFB80_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32666.1"
                     /translation="MTLVGKIFTVLILVMSIMFMAFSMMVFATHKQWKDHAGKLTKEL
                     AELKTAQNDAKALEQRLRNELAQEQAARKSVLAALQQRATKAETDLVAKQQELDNISA
                     QHTKTVAEAEAAQQRLLALETETKKLRDELRTVQRDLDVQFNRVVKLTDDLNQAESLK
                     QILDERLREATFHMAKMKEVMTKNGLTETSLVDHIPPRVEGIVLEVSDKDLIEISIGN
                     DDGLKIGHTLDIYRDNTYLGRIVIRKTGPDRAVGQVQKEFQRGQIKRGDRVTTKFS"
     gene            3490..3666
                     /locus_tag="VFB80_02575"
     CDS             3490..3666
                     /locus_tag="VFB80_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32667.1"
                     /translation="MSLPSSANQPIAEKPGTNVYTVMLILSFCAIVVACTLLYVELTR
                     FGNYPWWQTGAGGS"
     gene            3824..>4664
                     /locus_tag="VFB80_02580"
     CDS             3824..>4664
                     /locus_tag="VFB80_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32668.1"
                     /translation="MSLAARWIESGEQPADFYELAGQARFHPRAEEVVAALSALATEA
                     RGYQGHWRAELGARSQQVQERIQQAIGVFSSPQTRQHYDEQLWQQIRVAFTKEFSALG
                     PAASGNAVRRWLRMMQHVHPSRLEESVVRLTRGGPAPAAASKSATSTVACAKDATQPA
                     PPKAAPAPAESPPAAAKPRPPAFRGQAKPESPPDDYGAIWIVGSAILTTIVAVVALLY
                     FFGNRFGSQPSVAQVQPLQPVPPQPAPPRAPAPAATPAPPIPAPTPPPPAPEPEPAPT
                     PPPP"
BASE COUNT          786 a         1592 c         1508 g          778 t
ORIGIN      
        1 tggtcggaat tttcaagctg cgcgtaattt ctgccgcggc cgacgccggg atgcactcga
       61 cgacgatcgc gaacgcaccg gcctcctggg ccgcggcggc gtcggccatc agacgctgcg
      121 cgtcgcgctg gacgcggtag ccgcccatct ggtggacgct ctgcggccgc aagccgacgt
      181 gcgccatgac gggaatgccc gcgctgacca gaccgcggat gacgtcggcc tggtcggcgc
      241 cgccttcgag cttgacggcc tggcagcggg cctccttgag aatccgcccg gcactctcga
      301 tggcccggtg gacgcccaga tggtaggtcg ggaagggcag atcgacgacg accagggccc
      361 gctcgaccgc gcggccgacc atctcggcgt gatagatcat ctcgtcgagc gtgacgggca
      421 gcgtggtgtc gtgcccctgc acgaccatcg acatgctgtc gccgacgaga atcgcgtcga
      481 tccccgcccc gtcgagcagc gccgccgtag gataatcgta ggccgtgagc atgctgatcc
      541 gccggcctgc ggttttgaag gcgatgaact ccggaacggt cacgcgggac gagcgttttg
      601 ccataggagc ggccggcggc ggagcattac agtaaagaac tcactattgc attgtgactg
      661 gcggcgtccg gagtgacaac gagtgggcgc ggccgaagat ccgacatgtt gaaagcagcg
      721 ccacgccgat cgctcgcacg ccctagcgcc gcagcgccgc aggaggcaga gcccgcggcg
      781 cgctggcggc cgcagttttt gaccaccctg ggctgcggcg cggcgggggc gctgctgctc
      841 tgggccgcgt ttccgccgct caactggccg ctgctcgcct ggctcgcgcc ggtgccttgg
      901 ttgtggctca tccggctgcc ggcgctgccc ggctggcggc cctacttcat gctctggctg
      961 gcgggcagtt tccattggct gcttgtgctg cagggcattc gcctggccca tccggcgctg
     1021 tacggcggct ggattgccct gtcgctctac ctggccgtct atctgcccgt gttcatcggg
     1081 ctgacgcggg tggccgttca ccgcctgaaa gcgccgctcg tcgccagtgc acccgtcgtg
     1141 tgggtcgggc tggagctgct ccgcgggcat ctggtcacag gattctcgaa cggactcttg
     1201 gcccacaccc aggccgagat tccgctgctc attcaagtgg ccgatctggc cggcggctac
     1261 acgctgagct tcgtcctcat gttcgttgcc gcctgcctgg cgatgctgat tccagttgcg
     1321 ggtgcctgtg gtccgcgcag cggcccacag cccgcgcgga cgtggagcgt tgtctggccg
     1381 ccgctgctca tcgccgccgt gatgctcgcc acgctgctct acggcaactg gcggctggcg
     1441 cagaaaacgg ctgccggtcc aaccgccaag gttgcgctcc tccaaggagc gctggacacc
     1501 gtatttggcg aggatcgcag cgccgaaacg ttcgagcatt atcgccagct gacggaccag
     1561 gccgtcgcag gcagggcgga cctcgacctg gtcgtctggc ccgagtcgag ctttcccgcg
     1621 cccgaagtgc tgatcgaaga gcccctcgca ccgctcccga atatgtcggt ctcgcagttg
     1681 cgttcgaatg cggacgtcgc ccagcggcag ctgcgcgacc tgctcgccgg cgaagcgttg
     1741 cgggccaatg ccaatacgaa ctcgggagcg gcgggcacgc ggctgctggt ttgcggcacg
     1801 agcttcgtct atgggccagc gcagccgcga atgtacaacg cggccctcgt gatcgatccg
     1861 cgcggcgaca tggcttgccg ctattacaaa acgcatccgg tgatgttcgg cgagtacatt
     1921 cccttcggcg aggcgtttcc ctggctctac aagatccagc cgctgggcat gggcctgtcg
     1981 cgcggcgacg ggccgcgggt gttcgaggcc ggcggcctgc ggatgagccc caatatttgc
     2041 ttcgagagca cggtccccca cctggttcgc ggccaattgc gggagctgtc gcgccgcggc
     2101 acgccagctg atgtaattgt caacgtcagc aacgacggct ggttctgggg ctcggcgatc
     2161 ctcgacctgc acttccgctc gaacatcttc cgcaccgtcg aaaaccgccg cccgctactg
     2221 gtcgccgcga acaccggcat ctccgcctgg atcgactcca gcggcgtcgt ccgccagcgg
     2281 ggccagaagc gcacgcccca ggtcctatat gcggagattc aggcggacgg ccgcgccagc
     2341 ccgtacagcc tgatcggcga ctggccagcc tggatttgcg ccgcggcatg tgccggcctg
     2401 gcactcgcgg gatttcgccg ccgggcggaa taaccggact gctcggcatc gaataaatgg
     2461 gcagcggcca agattgagca gcccgcaggc gagtaccctc ccaagtcacg cagactgccg
     2521 attttgccgc gattcgttga cttttctttt ggcccggaat tatactgcgg gggtaacttc
     2581 ggaccggctt ctctcggcct gcgggcccac ccgcagccat cctaactgct tgctgccaga
     2641 gaagtttcgc tcgaagtgcc tatcgaccat tcaggggacg gtgagccatg acgcttgtcg
     2701 gcaagatctt cacggtgctc attctggtga tgagcatcat gttcatggcc ttttccatga
     2761 tggtcttcgc cacgcacaag caatggaagg atcacgcggg caaactcacg aaggaactgg
     2821 cggaactaaa gaccgcgcaa aacgacgcca aggcgctcga gcagcggctc aggaatgaac
     2881 tggcccagga acaggccgcc cgcaagtcgg tgctggctgc cctgcagcag cgggcaacga
     2941 aggccgagac cgacctcgtc gccaagcagc aggagctcga caacatctcg gcccagcaca
     3001 ccaagaccgt cgccgaggcc gaagcagccc agcagcgctt actggcattg gagaccgaga
     3061 ccaagaagct ccgcgacgaa ttgcggaccg tgcagcgcga cctggatgtg cagttcaacc
     3121 gcgtcgtcaa gctgacggac gacctcaatc aggccgaaag cctgaagcag attctcgacg
     3181 agcggctgcg cgaggcgacc ttccacatgg ccaagatgaa ggaagtgatg accaagaacg
     3241 gcctcaccga gacgagcctg gtcgatcaca ttccgccgcg ggtcgaaggc atcgtcctgg
     3301 aagtcagcga caaggatctg atcgagattt cgatcggcaa cgacgacggc ctgaaaatcg
     3361 gccacacgct cgacatctat cgcgacaaca cctatctcgg ccggatcgtc attcgcaaga
     3421 ccggtcccga ccgcgccgtc ggccaggttc agaaagagtt ccagcgcgga cagatcaaac
     3481 gaggcgaccg tgtcactacc aagttcagct aatcagccga tcgcagagaa gcccggcacg
     3541 aacgtctaca cggtcatgct gattctctcg ttctgcgcga tcgtcgtggc ctgcacgctg
     3601 ctgtacgtgg agctcacgcg gttcggcaac tatccctggt ggcagaccgg ggcgggcggc
     3661 tcgtaagcgt aggccggatt gttaatccgg ccgagcgtgg acggattgac aatccgtccc
     3721 acagcaactt gcattcggcc gggccgtgcc gagaatcggg atataggcgg cttgcggccc
     3781 agcggctgcg cggcctctca tcctgctttc ccgcgcgatt cccatgagcc tcgctgcacg
     3841 ctggatcgag tcgggcgagc agccggcgga tttctatgag ctggccgggc aggcccgctt
     3901 tcatccccgc gcggaggagg tcgtcgccgc gctgagcgca ctggccaccg aggcccgcgg
     3961 ctatcagggg cactggcggg cggagctggg cgcgcggtcc caacaagtgc aggagcgaat
     4021 ccagcaggcc atcggagtct tctcgtcccc ccagacccgc cagcactacg acgagcagct
     4081 ctggcagcaa atccgcgtcg cctttaccaa ggagttctcc gccctcgggc cggccgccag
     4141 cggcaacgcc gtgcgccgct ggctgcggat gatgcagcac gtccacccct cgcggctgga
     4201 ggaatcggtc gtccggctga cgcgcggcgg cccagctccc gcggccgctt caaagagcgc
     4261 gacgagcacg gtcgcctgcg ccaaggacgc gactcagccc gctccgccca aggccgctcc
     4321 cgcgccggcg gaaagcccgc cagccgccgc caagccgcgg ccgccggcgt ttcgaggcca
     4381 ggcgaagcca gagtccccgc cggacgatta cggcgcgata tggatcgtcg gctcggcaat
     4441 tctgacgacg attgtggccg tcgtcgcgct gttgtacttt ttcggcaacc gtttcggcag
     4501 ccagccgagc gtggcccagg tccagccgct gcagcccgtg ccgccacagc ctgcgccgcc
     4561 aagggcgccc gcgccggcgg ctacaccggc gccgcccatt ccggctccga ctccgcctcc
     4621 ccccgctccc gagcctgagc ccgcgcccac tccaccgcca ccag
//
LOCUS       DASRJB010000089         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_874161, whole genome shotgun sequence.
ACCESSION   DASRJB010000089 DASRJB010000000
VERSION     DASRJB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874161"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFB80_02585"
     CDS             complement(<1..591)
                     /locus_tag="VFB80_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN32669.1"
                     /translation="MKRPAMLVDCITSPDDPLLDRFCAKLAELAPQLEKLGAWPGEQL
                     RLCGEAGVHEWFVGSPHGGQGWSEADVLRGYVKLAAACLTTTFVLTQRTGAMTRIAAG
                     DSDVARERLLFDLVEGRSFATVGISHLTTSRQHVAPVMRAEERGEDFILDGYSPWVTG
                     AEHAQHIVLGATLADRRQILVALPTDLAGVRIPPAPE"
     gene            726..2417
                     /locus_tag="VFB80_02590"
     CDS             726..2417
                     /locus_tag="VFB80_02590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZN32670.1"
                     /translation="MTHRPMLTIDDLKAERPQYDAVAAKYKALEAELAVATQAAAAIG
                     VVERWDQLRRELETWSSLVYLRFNQDTADPQFKADREYCDEITPRLTDLEVRIKRLLL
                     ASPHRKDLERKFGPQAFALWEADVMAFDPKIEQHLVRESKLQAEYSELTAAAQVEFRG
                     EKHNLSSIVKFREDADRQTRHDAERVRWQWFADNRPALDRIFDDLVKLRHDMARQLGF
                     ADYIGLGYKRMKRVDYSQADVERFRAEVREHLVPLAAEIRRQQAAALGVERLMYWDDA
                     VHDPRGNPLPHGDHDWMVERAQKMFDAMSQSLGGFFQLLRQSRLTDLKNRDRKSPGGF
                     CTAFPSYGLPFIFANFNGTKHDVEVFTHEMGHAYQAYASRQQPLLDYLWPTYESCEIH
                     SMGLEFLTWPHMEQFFEADAERFRKLHLTQGLLFIPYGVAVDHFQHLVYARPDAAPAE
                     RHAMWQEMERLYLPWRDYGDLPHVGSGGFWQFQRHIYLSPFYYIDYTLAQTCALQLWV
                     RAQRDPQGTLAAYDRLCARGGEAPFQDLARSAGLVSPFSPGCLRDVVAQARAALA"
     gene            complement(2438..>3509)
                     /locus_tag="VFB80_02595"
     CDS             complement(2438..>3509)
                     /locus_tag="VFB80_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32671.1"
                     /translation="GVCIEDFDAGVLSIATGNDSDDVRVRDAGIGTLVITTGSGHDSV
                     SVGEVDIEFAASATTGAGDDDVEFIEFNVGLGLTILTENGLDDVTVEAASIGGSLVIQ
                     TGNDADEVSIEGIDVAGAIVVNTAGGNDEVHLQSFDAQSVVIDTGAGNDGSPTGDSPI
                     TVENAEIDFGLSIFTGDGRDLVLVQDSVALIVVINSGNHDDDVDFYNSDTTLDLIINT
                     GSGVDFVDIQGGEGGFVDIGRDLIVDTGAHNDELEVSDTNVRRNAAISLGAGNDELFV
                     TTVHVRGNAVIDAGAGADFVQIDESDVDKLLAVYMGAGNDELTIRNSSAKKALLFGGP
                     GLDDLLLDNVDADESHVFEFED"
BASE COUNT          611 a         1154 c         1144 g          600 t
ORIGIN      
        1 ctccggcgcg ggcggaatcc gcacgccggc caggtccgtc ggcaacgcca cgagaatctg
       61 ccgccgatcg gccagcgtcg cgcccaagac aatatgctgg gcgtgctccg ccccggtcac
      121 ccaaggcgaa tagccgtcca aaatgaaatc ctcgccgcgc tcctccgccc gcatgacggg
      181 cgcgacgtgc tgccggctgg tcgtcaggtg cgaaatgccg accgtggcga acgagcgccc
      241 ctcgaccagg tcaaacagca gtcgctcgcg ggcaacatcg ctgtcgccgg ccgcaatgcg
      301 ggtcatcgct ccggtgcgct gcgtgagcac gaacgtcgtg gtcaagcagg ccgccgccag
      361 cttcacatag ccgcggagca cgtccgcttc gctccaaccc tgcccgccgt gcgggctgcc
      421 gacgaaccac tcgtgaacgc ccgcctcgcc acacagccgc aattgctcgc ccggccaagc
      481 ccccagcttc tccaactgtg gcgctaactc cgcgagctta gcgcaaaacc tatccagcag
      541 cggatcgtcg ggcgaggtga tgcagtcaac taacatcgca gggcgcttca ttggctacat
      601 tatatcgact gtagggtgtg accaggcggg caccgccgcg aagcggtggt cggcggccga
      661 cggcacacca tgagggcgct gatggtgggc cggcgctcgc tgtcgctcgc tcgtcctacc
      721 ctacagtgac gcaccgacct atgctcacca tcgacgacct gaaggccgaa cgtccgcagt
      781 acgatgcagt ggccgcgaag tacaaagcgc tggaggcgga actggcggtg gccacgcagg
      841 cggcggcggc gatcggcgtg gtcgagcggt gggaccagct gcggcgcgaa ttggaaacgt
      901 ggtcgtcgct ggtttatctg cgattcaatc aggacaccgc cgacccgcag ttcaaggccg
      961 accgcgagta ttgcgacgaa atcacgccgc ggctgacgga cctggaagtg cggatcaagc
     1021 ggctgctgct ggccagcccg caccgcaaag atctggagag gaagttcggc ccgcaggcgt
     1081 ttgctttgtg ggaggcggac gtgatggcgt tcgatcccaa gatcgaacag cacctggtgc
     1141 gggagtcgaa gctgcaagcc gagtacagcg agctgaccgc ggccgcacag gtggaattcc
     1201 gcggcgagaa gcacaacctg tcgagcatcg tcaagttccg cgaggacgcc gaccgccaga
     1261 cgcgccacga tgccgagcgc gtccgctggc agtggttcgc agacaaccgg ccggctctcg
     1321 atcggatttt cgacgacctg gtgaagctgc gccacgacat ggcccggcag ctgggctttg
     1381 cggactacat cggcctgggc tacaagcgga tgaagcgggt cgattattcg caggccgacg
     1441 tcgagcggtt tcgggccgag gtccgcgagc acctggtgcc gctggcggcc gaaattcgcc
     1501 ggcagcaggc cgcggcgctg ggcgtcgagc ggctgatgta ttgggacgac gcagtccacg
     1561 acccgcgcgg caatcctctc ccgcacggcg accacgactg gatggtcgag cgggcccaga
     1621 aaatgttcga cgcgatgagc cagagcctgg gcggattctt ccagctcttg cgccagtcgc
     1681 ggctgacgga cctgaagaac cgcgaccgca agagccccgg cggcttttgc acggcgtttc
     1741 ccagctacgg gctgccgttc atcttcgcca acttcaacgg caccaagcac gacgtggagg
     1801 tcttcacgca cgagatgggc cacgcctatc aggcgtatgc cagccggcag cagccgctgc
     1861 tggactatct gtggccgacg tacgagtcgt gcgagattca ttcgatgggc ttggagttcc
     1921 tgacctggcc gcacatggag cagttcttcg aggccgacgc ggagcggttc cgcaagctgc
     1981 acctgacgca gggcctcttg ttcattccct acggcgtcgc ggtcgatcac ttccagcacc
     2041 tggtctatgc gcggccggac gccgcgccgg ccgagcggca cgcgatgtgg caggagatgg
     2101 agcggctcta tcttccgtgg cgcgactacg gcgacctgcc gcacgtgggc agtggcgggt
     2161 tctggcaatt ccagcggcac atctacttgt cgccgttcta ctacatcgac tacacgctgg
     2221 cgcagacctg cgccttgcag ctctgggtgc gggcgcagcg ggacccgcag ggaacgctcg
     2281 cggcgtacga caggctctgc gcccgcggcg gcgaggctcc gttccaggac ctcgctcgct
     2341 cggccggcct ggtcagcccc ttctctcccg gctgcctgcg cgacgtggtt gcgcaggccc
     2401 gcgcggcgct ggcttagttc gcaaagagcg gttcggccta gtcctcgaac tcgaagacgt
     2461 gggattcatc cgcgtccacg ttgtcgagta gcaggtcgtc gaggccgggg ccgccgaaca
     2521 gcagggcctt cttggcggag ctgttccgga tggtcaactc gtcgttgccc gcgcccatgt
     2581 agacggcgag cagcttatcc acgtcggatt cgtcgatttg gacgaaatcg gcaccggcgc
     2641 cggcgtcgat cacggcattg ccgcggacat ggacggtggt aacaaacagc tcgtcgttgc
     2701 ctgcgcccaa ggagattgcc gcgttccgcc tgacgttcgt gtccgagact tccagttcgt
     2761 cgttgtgggc ccccgtatcg acaatcaggt ctcggccgat atcgacgaag ccgccctcgc
     2821 cgccctggat atcgacgaag tcaacgccgc tgccggtatt gatgatcaag tcgagcgtcg
     2881 tatcgctgtt gtagaagtcg acgtcgtcgt catgattacc gctattgatg accacgatca
     2941 gggccacgct gtcctgaacc agcacgaggt cgcggccgtc gccggtgaag atggacagcc
     3001 caaagtcgat ctcggcattc tcgacggtga tcgggctgtc gccggtcggc gagccgtcgt
     3061 tgccggcgcc ggtgtcgatg acgaccgact gggcgtcgaa cgattgcaga tggacctcgt
     3121 cgttgccgcc ggctgtgttc acgacgatcg cgccggcgac gtcgatgccc tcgatcgaga
     3181 cctcgtcggc gtcgttgccc gtctgaatca ccagcgagcc gccgatgctg gccgcctcaa
     3241 cggttacatc gtcgaggccg ttttcggtga ggatcgtcag ccccaacccg acattgaact
     3301 caataaactc cacatcgtcg tcgccagcac cggtggtggc cgaggcggca aattcgatat
     3361 cgacttcgcc gaccgacacg ctgtcgtggc ccgagccggt ggtgatcacg agcgtgccga
     3421 tgcccgcatc gcggacgcgc acatcgtccg aatcgttgcc ggtcgcgatg ctcagcacgc
     3481 ccgcgtcgaa atcctcgatg cagacgccg
//
LOCUS       DASRJB010000090        14948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3976250, whole genome shotgun sequence.
ACCESSION   DASRJB010000090 DASRJB010000000
VERSION     DASRJB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3976250"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(554..1618)
                     /gene="purM"
                     /locus_tag="VFB80_02600"
     CDS             complement(554..1618)
                     /gene="purM"
                     /locus_tag="VFB80_02600"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZN32672.1"
                     /translation="MAKATYKDAGVDLDVYDEAMTRLPRLMHRTYSPRVLKLDGGFAG
                     LFQLDFASRLFERKYENPILVSCTDGVGTKLKVATLVDKHDTVGIDLVAMSVNDALCC
                     GAEPLFFLDYVAMSHDDPERLEQLVRGVSDGCIEADCALMGGETAIMPDIYARGEYDL
                     AGFCVGVVERKHLIDGRSISPGDLVLGIASSGLHSNGYSLIRKVVFDLAGLGVHDQVP
                     ELGTTVGEALLTPTRIYSRPIRKILQYYKVKNVVHGIAHITGGGLRDNLERILPENTQ
                     AVLEKGSWPIPPVFTWLQSLGGIEDAEMERVFNLGIGLVLVISPYYEATISSMLADAG
                     LDHWKLGEMKTGPKSVVWRQ"
     gene            complement(1984..2598)
                     /locus_tag="VFB80_02605"
     CDS             complement(1984..2598)
                     /locus_tag="VFB80_02605"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZN32673.1"
                     /translation="MPGEPTTIYSAANSQQAYLLKGLLEERGITAWVVNDALQIAGGE
                     LPLGWTAAARVVVSSSDAAAAREFALQFDRQTAHEPAPDDSDQEPPPAEWANWPTCPK
                     CQERRSAHCPVCGASGTRFPLADTQQTEGGERVLLYCQSCDDHMLPQWHRLCPRCGHD
                     FGDGLDLNRPPAKWSLNARSWLVIAGLSAGVAIFVAYCVWLFSR"
     gene            2733..3776
                     /locus_tag="VFB80_02610"
     CDS             2733..3776
                     /locus_tag="VFB80_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZN32674.1"
                     /translation="MPFDGSPRFLIARMSAIGDTVLTLPVLCALRQAFPQAFIAWVVE
                     RAAAPMVQGHDALDELIVLPRLWLKSPLEIWRLRRRLRAMRIDTTIDPQSINKTAIAC
                     WLSGAKQRIGLGGFYGKELSTWLNNVRLVNRQSHITDRSLELLEPLGVRPSPAQFRVP
                     HNAPAEATIAAFLAASELAHGFAVVNPGASWDSKLWPSERYVEVVRHLCAKHNLRSVA
                     VWAGDRERAWAEQIVARLPASALLAPKTDLLELTALVRRARIFVGSDTGPMHTAAAVG
                     TPCVALYGTTRPEDCGPYGPQHIALQSRYDDGTRSQRRKATNEAMSLITAAQVTAACD
                     QILKRPAQPRAAA"
     gene            3802..4422
                     /locus_tag="VFB80_02615"
     CDS             3802..4422
                     /locus_tag="VFB80_02615"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZN32675.1"
                     /translation="MSMLELAALRETTLISEPFEHLIVPRFVKAECLEAIERDFPPIA
                     SGGSFPLNSLHYGPAFEELTDELLGDDVRQVFAEKFAMDLSERPATLTVRGRTRLKDG
                     QIHIDSKTKLITVLLYLNRHWTAGGGRLRLLRSEHDLEDVVAEVPPDQGALVAFRCRP
                     NAWHGHHPFDGERRSMQLNYVVSETASRWSSLRHSVSALFKSRRAA"
     gene            complement(4473..6023)
                     /gene="rpoN"
                     /locus_tag="VFB80_02620"
     CDS             complement(4473..6023)
                     /gene="rpoN"
                     /locus_tag="VFB80_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZN32676.1"
                     /translation="MRLSFGLEHRQLQVQKLAPRMIQSMEILQLPLQELQERIEQELV
                     ENPTLELQERDPTLPDEPVEQENPLEKPIEDKPLVVDEAHHNADDFERLLNLDRDVPE
                     YFEESTRRSSNRIEEDSERAHDVISNVEERPESLQDYLVHQLGEMELPPELFALCERI
                     ISTLDPRDGGYFRASLRDLLPPDAAQEQLQTAEKALSIVQSLDPPGIAARDLRECLLA
                     QLDPDMDFYEELHTLISNHLEDLRDNRLPLIERVTGLSIEEIQEAWSELRKLNPKPGA
                     RFADAVVPTVTPDVTVERSDDGQYKVHVDESRTPTLYISRHYRERLASGTATPEEREY
                     IKRKINAAQWLIESIEQRRNTLTRVSQAIVDHQTRFLDDGPEFIEPLKMQQIADKVGV
                     HVTTVSRAVDDKYIQTPRGIFPLKRFFVGGTKSDEGEDVAWDIIRIRLQEIVDKEDKT
                     KPYSDDELVEELKKQGLVVARRTVTKYRQKMDIPSSRQRRDWSKTAAAPSVHPAIPPE
                     AVVAPATH"
     gene            complement(6060..6659)
                     /gene="recR"
                     /locus_tag="VFB80_02625"
     CDS             complement(6060..6659)
                     /gene="recR"
                     /locus_tag="VFB80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZN32677.1"
                     /translation="MTQITQSVGRLIDELAKLPGIGRKSAERLAYHLLRVHQNEALAL
                     ADAIRDVRSNVHYCSICFNLAEGERCAICTDPKRDPTQICVVEQPRDLMALEQAATFR
                     GHYHVLLGRIAPLDGVGPEQLTIDRLVERVREGGVREIIMATNPTVEGDGTALHISNM
                     LAEYPVQITRLARGITTGSILEYTNREVLADALSGRQRF"
     gene            complement(6700..8409)
                     /gene="dnaX"
                     /locus_tag="VFB80_02630"
     CDS             complement(6700..8409)
                     /gene="dnaX"
                     /locus_tag="VFB80_02630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZN32678.1"
                     /translation="MADREGAGQYIVVARRYRPQDFAQLVGQGQVAQALANAITTNRV
                     GHAYLFTGARGVGKTSTARIFAKALNCVRGPTTEPCGECDVCQGIASGDDVDVIEIDG
                     ASNRGIDEIRQLRSTVNVRPSRARFKIYIIDEVHMLTTPAFNALLKTLEEPPEHVKFI
                     FCTTEADKIPITVLSRCQRFDFAPIEMRSIVERLQHICASEGVSAEPEALQIIARRAA
                     GSMRDSQSLLEQLLSFGGKQITVADVHAMLGTAHSARLTALAQCVARRDAAAALAEVE
                     AAVVEGVDLGAFTEQLLGYFRDCLAALVGCTPELMLHTAAADHPEVKKLGETLGLETL
                     LAMAQVLDHTLTRLRQSTHVRTLVEVALVRLCKLEDLDALPGLVAALRDGAPPPDAGT
                     KTTAKHTPTAEADPPSAQKKTSENGDAPGVVAVESTPVLDASTAVSAWKQTLAEIGDM
                     TADFAGRADGVAISGPNRLVVRFRKAYTQALQYCERPDKRQRLEQTLSRITGRNIRID
                     FALVQEESAAPAPEPPRPINRRQRQQELMRHPLIRKAAELFDTEIVAVLEPPPAESGP
                     AEA"
     gene            complement(8430..8525)
                     /gene="ffs"
                     /locus_tag="VFB80_02635"
     ncRNA           complement(8430..8525)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFB80_02635"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8606..10156)
                     /locus_tag="VFB80_02640"
     CDS             complement(8606..10156)
                     /locus_tag="VFB80_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN32679.1"
                     /translation="MSTFRWPLQAPILAAILAVLLAPRFADAADRAAAQAALASITKE
                     ELKSHVDVLADDTFEGRETGSRGGRAAGNYVLKAFETLGLKPAGEDGTYLQSFGSSRN
                     ILGLVEGGDPQLKSQFVVLGAHYDHVGYGRSTNSYGPWGYIHNGADDNASGVAGLLEI
                     GDALKRLPAVPRRSILLACWDGEEAGLLGSRHWISRPTVPLARVAATINLDMIGRMQD
                     GKLEVLGSRTAAGLRRLVSESNGEARATVDFDWSLKADSDHWPFYEQKIPFLMFHTGL
                     HADYHRPSDDAHRINHAGLLGATQIAFQTVLALADAEQLPAFREAVRRESSWGRVALE
                     QPAAAQPPRFGIPFKVESADPLKIVLTGLTPGSPAESAGLKAGDELLQLQGRPIESEQ
                     LFRLHLLAAAGETAFLVKRPGSDTPLLIKVTPRGDPIRVGLSWRFDDAEPGSALVTHV
                     ISSSAAQLAGLKTRDRIYAVGGHSFQSEADFVNLLSTVPSPMDLAVERDGKVEMRKLE
                     LLDFAPAP"
     gene            complement(10251..11342)
                     /locus_tag="VFB80_02645"
     CDS             complement(10251..11342)
                     /locus_tag="VFB80_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32680.1"
                     /translation="MTIGFGIIGCGMISRFHAKAVADIKGAQVAACFDSFTSAADKFG
                     AEIGCQVYHDLDAMLADKSVDVVTICTPSGVHMEPAVAAAKAGKHIIVEKPLDITLKR
                     CDAMIAAADKAGVVLSTIFPSRFHESSQLLKGAIDKGRFGKLTMGDAYVKWFRTQEYY
                     DSGAWRGTWALDGGGALMNQAVHSVDLLLWFMGPAVEVTAYTATLAHERIEVEDVAVA
                     TVKFASGALGVIEASTATWPGELKRIEISGNAGSVVLREEDLAVWKFAKETKADDAIR
                     EKMGSRTHTGGGAADPKAIGHHGHTLQFQDVLNAIKKGKQPLIDGPEGRRAVELILAI
                     YKSAESGKPVQLPLAADPVLKGRSKGKKL"
     gene            complement(11406..12551)
                     /locus_tag="VFB80_02650"
     CDS             complement(11406..12551)
                     /locus_tag="VFB80_02650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN32681.1"
                     /translation="MLTPAVAGLTAVIVVGLFACGWCWIIAGLRAGLTLGWITGDAAD
                     AVLLVLRPLRVPPRLPLVEWSPRRPVPWALFDLFALVGIWLGSQLAIAGLLRDTGWIP
                     RGSDLKTLTLDQRQAFIVANVAVSLAILAIGLPLISLRTGAKTRDFGFSLTDVPRDIG
                     RGLIGLVMLAPPVYVLQAILVTVWKPSSHLLVELFKGTPNPVFFIVLFVAAAIVAPLF
                     EELMFRVILQGFLERAFTHRGPLWELLFDRPRGLPLAVAAEPLPKSLEPPVAAVLAPS
                     TGGLNPYDSPQDVSEPEFVAEVVDDQQPELRGPAAWLPIGISAIVFALMHWSHGPDWV
                     PLTFLAAGMGYLYQRTHRLVPSLIVHAGLNALSMWMLWVHVFEGVKV"
     gene            complement(12553..13146)
                     /gene="pgsA"
                     /locus_tag="VFB80_02655"
     CDS             complement(12553..13146)
                     /gene="pgsA"
                     /locus_tag="VFB80_02655"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZN32682.1"
                     /translation="MSQSNVIFNVPNILTALRLALSIGVFVLIPLEQFLAATIVFVLA
                     AGTDWIDGWWARKYSQVTKLGRIFDPFVDKILICGTYIYLAAIPRSGILPWMAVVVMG
                     REMLVTVIRSFIEEQGGDFSAKMAGKLKMVFQCVAAVASLVALRHFELAGSTSAALPP
                     WLSWTLTISVWLAVISTVYSGLEYVFAAGRILSQQGR"
     gene            complement(13197..14627)
                     /gene="rimO"
                     /locus_tag="VFB80_02660"
     CDS             complement(13197..14627)
                     /gene="rimO"
                     /locus_tag="VFB80_02660"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZN32683.1"
                     /translation="MKNSKKSAKDSKGTYAFVSLGCPKNLVDSERMLGLLQIDGYELV
                     SEPAGADFVVVNTCGFIERARAESFQAIDEMLELKKQGGTRGVIVSGCLAERQREQLL
                     EERPDIDHLVGVFGREEITRVADRLVGRLEEQRTVFRPAPIRALSDRDRLRITPRHFA
                     YLKISEGCDRLCTFCAIPKMRGKHASKPMEEVVLETEQLAADGARELIVVAQDTTYYG
                     LDLYGKPRLAELLRRLEGVAGIDWIRLMYFYPMYIDDELINTIADGPKILPYIDIPLQ
                     HASDSMLKRMSRRVTRPETEDLLGRLRSRIKDLTLRTTFITGFPGETDDDFAQLCDFV
                     ERHRFERMGVFTYSLEPDTPAAKLPDHVPAAVMEERREELMAIQQQIAFERNAAQVGR
                     TLDAILDQPVEGEKNVWLGRTAADAPDVDGLVFVTGDQHKLRAGDIVPVEIVATQDYD
                     LIGVAAGRPKARHAPPSKAAAGLVQV"
     gene            14763..>14948
                     /locus_tag="VFB80_02665"
     CDS             14763..>14948
                     /locus_tag="VFB80_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZN32684.1"
                     /translation="MNDALGILEVTGLTPAMAALDAMDKTAGVRLLQTESNDFYGVVL
                     KVTGAQANVAAAIAAGKA"
BASE COUNT         2610 a         4819 c         4765 g         2754 t
ORIGIN      
        1 gccgagcgca ggaagggcac ttccaagagc gtgatgctca cgcctccgga ctcgccagtc
       61 agctctggaa tcgtgaccgt gtcgaacgtc ccctttctcg gcataagcct ctccggtgtt
      121 ggtagcctga cccccttccg cttgtacccc cttccgcgtg gcaccttgtg accccttccg
      181 cttcgcccct tccgcttcgc atcgtggtga tgctatccat ggcgtttccg tgagtctgtt
      241 agatcattgt ttcgttcgat gtcgatagtc tttaacccgc gcttctgaag aatagtcgcg
      301 acgattgcaa tgacgcccga aagaagcgta atcaatagcg ccggggctac cacacctgtg
      361 atgtcctctc ctgtcccacg agccaaaaac attactgtag caattcctgc caccatcaag
      421 ccacaaacag cgatgccagc gtatctcacc gtctttagaa caagagttga atcttctctc
      481 attttgcacc tcacaatggg tcaggctccg ttattctcat tcttggctcg tcagtctggg
      541 tcgctcacgg cggctactgc ctccaaacca cggatttggg gccggtcttc atttcgccga
      601 gcttccagtg atcgaggccg gcgtcggcga gcatgctgct gatggtggct tcgtaatagg
      661 ggctgatcac gagcaccagg ccaatgccga ggttgaagac gcgctccatc tcggcgtcct
      721 cgatgccgcc caggctttgc agccaggtga agaccggcgg aatgggccag gagcccttct
      781 cgagcaccgc ctgcgtgttc tcgggcagaa tccgctccag gttgtcgcgc agaccgccgc
      841 cggtgatatg ggcgatgccg tggacgacgt tcttcacctt gtagtactgc aggatcttgc
      901 ggatcggccg cgagtaaatt cgcgtcggcg tgagcagagc ctcgccgacc gtcgtgccca
      961 gctctggcac ctggtcgtgc acgcccaggc cggccaggtc gaacacgacc ttgcggatca
     1021 ggctgtagcc gttgctgtgc aggccgctgc tggcgatgcc caggacgagg tcgccgggcg
     1081 aaatcgagcg gccgtcgatc aggtgcttgc gctcgacgac gccgacgcag aacccggcca
     1141 ggtcgtattc gccgcgggcg tagatgtcgg gcatgatcgc cgtctcgccc cccatcaggg
     1201 cgcagtccgc ctcgatgcag ccgtcgctca cgccccggac gagctgctcc agccgctccg
     1261 gatcgtcgtg cgacatggcg acgtaatcga ggaagaacag cggctccgcc ccgcagcaga
     1321 gggcgtcgtt cacgctcatc gccacgaggt cgattcccac cgtgtcgtgc ttatcgacga
     1381 gcgtcgccac cttgagcttc gttcccacgc cgtcggtgca ggagacgagg atcgggttct
     1441 cgtatttccg ctcaaacagc cggctggcaa agtcgagctg aaacagcccc gcgaagccgc
     1501 cgtccagctt caggacccgc ggcgagtacg tgcggtgcat cagccgcggc agccgggtca
     1561 tggcctcgtc atagacgtcg agatcgacgc cggcgtcttt gtaagtggct ttggccattg
     1621 actttacgga aaatggacgg cgactggcag ccctcgcggc ggggagtgca acggccgatt
     1681 gtcgatggca cttgtcgagt tgtcaacgca cgggcgtaac ccattgagat cagtagtgta
     1741 cattggtatg aaatttgcac cattgttctg tctggcgcgg cggactgccg tttcggcgga
     1801 tgtcgcctgc ccagacgccg ccgaatgacg cgttctctaa ggctaataag tactatttaa
     1861 ggtggggacg agagttttga cgcaagtctt taagcggcaa tggcttgcaa tcacaaaaaa
     1921 gcgttttgag gcctttgcaa gccacccaac caggggccgc cttattagcg ccaaccaggt
     1981 cgatcaccgg gagaacagcc acacgcagta ggccacaaag atcgcaacgc cggccgacaa
     2041 gccggcgatc accagccacg agcgggcgtt gaggctccac ttggccggag gtcggttcag
     2101 gtcgaggcca tcgccgaagt cgtggccgca ccgcgggcag agacgatgcc actgcgggag
     2161 catgtggtcg tcgcagctct ggcagtagag aagcactcgc tcgccgccct cggtctgctg
     2221 ggtgtcggcc agcggaaaac gcgtcccgct ggctccgcaa accgggcagt gggccgagcg
     2281 ccgctcctga cactttgggc acgtgggcca gttggcccac tcggccggcg gcggctcctg
     2341 gtcgctgtcg tcgggcgcgg gttcgtgcgc cgtttggcgg tcgaactgga gggcgaattc
     2401 gcgcgccgcg gcggcgtcgc tgctgctcac gacgacgcgg gccgcggcgg tccagcccag
     2461 cggcagctcg ccgccggcga tctggagcgc gtcgttgacg acccaggccg tgatgccgcg
     2521 ctcctcgagc aggcctttga gcagataggc ctgctgcgag ttggcggccg aatagatcgt
     2581 ggtgggttcg cccggcatgc cggtttggcc cgaatgaaga acgaatggcg cgacgctagc
     2641 agcggccgtc gtggcaggca agttgacctt gccggctccc aggtgctggc atactctacc
     2701 gcgcccaaaa atcctgccca gcgaggatcg cgatgccgtt tgacggatcg ccccggttcc
     2761 tgatcgcccg catgagcgcc atcggcgaca cggtgctgac gctgccggtg ctttgcgcgc
     2821 tgcggcaggc gtttccccag gccttcatcg cctgggtcgt ggagcgggcg gccgccccga
     2881 tggtgcaagg gcacgacgcg ctggacgagt tgatcgtgct gccgcggctc tggctcaaga
     2941 gcccgctgga aatctggcgg ctgcgacgac gcctgcgggc aatgcggatc gatacgacga
     3001 tcgacccgca gagcatcaac aagacggcga ttgcctgctg gctgtcgggc gcgaagcagc
     3061 ggatcggctt gggcggcttc tacggcaagg agttgagtac gtggctgaac aacgtccgtc
     3121 tcgtgaaccg gcagagccac atcaccgatc gctcgctgga attgctggag ccgctaggcg
     3181 tacgtcccag tccggcgcag tttcgcgtgc ctcataatgc cccggcagag gcgacgattg
     3241 cggcgttctt ggccgcatcc gaattggcgc atggttttgc ggtcgtcaat cctggggcga
     3301 gctgggactc aaagctctgg ccgagcgagc ggtatgtcga ggtcgtccgc catctttgtg
     3361 caaagcacaa cctgcggtcg gtcgccgtct gggccggcga ccgcgagcgg gcctgggctg
     3421 agcagattgt cgcccggctc ccggcgagcg cactgctcgc gccgaaaacc gatttgctgg
     3481 agctgacggc gctcgtccgc cgggcgcgga tctttgtcgg ttccgatacc gggccgatgc
     3541 acacggccgc agccgtcggc acgccctgcg tcgcgctgta cggcacgacg cggccggaag
     3601 actgcggccc ttacggcccg cagcacatcg cgctgcagtc gcggtacgac gacggcacgc
     3661 ggagccagcg gcgcaaagcc acgaacgagg ccatgagcct gattacggct gcgcaagtga
     3721 ccgccgcctg cgatcagatc ctcaagcggc cggcgcagcc gcgggcggca gcgtagagca
     3781 ggcaatccca ggaggcagaa catgtcgatg ctcgaactgg cggcgcttcg cgagactacg
     3841 ctgatttccg agccgttcga gcacctgatc gtgccgcgct tcgtgaaggc ggagtgcctg
     3901 gaggcgatcg agcgggactt tccgccgatc gccagcggcg gcagctttcc gctcaactcg
     3961 ctgcactatg ggccggcgtt cgaggaattg acggacgaat tgctcggcga cgacgtacgg
     4021 caggtatttg ccgagaagtt tgcgatggac ctcagcgagc gaccagccac gctcaccgtc
     4081 cgcggccgca cccgcctcaa ggacggccag attcacatcg acagcaagac caagttgatc
     4141 accgtcctgc tttatctcaa tcgccactgg acggcgggcg gcgggcggct gcggctattg
     4201 cgcagcgaac atgacttgga agacgtcgtc gccgaagtgc cacccgatca aggcgccctg
     4261 gtcgctttcc gctgccggcc caatgcctgg cacggccacc atccgttcga cggcgagcgg
     4321 cgctcgatgc aactgaacta cgtcgtgagt gaaaccgcca gccgctggtc gtcgctgcgg
     4381 cattccgtgt cggcgctgtt caagtcgcgc cgggcggctt agcgcgtggc ggaggccacg
     4441 gcggcttagt gcgtggcagg cgccacggcg gcttagtgcg tggcaggcgc cacgacggct
     4501 tcggggggaa tcgctggatg gacgctgggg gccgctgccg tcttactcca atcccgccgc
     4561 tggcgcgagc tggggatgtc cattttctgg cggtacttcg tgaccgtgcg gcgggccacg
     4621 accagcccct gctttttcaa ttcctccacc agctcgtcgt cggaataggg cttcgtcttg
     4681 tcttccttgt cgacgatctc ctgcagccgg atgcggatga tgtcccaggc aacgtcctcg
     4741 ccttcgtcgc ttttggtgcc gccgacgaag aaccgcttca gcgggaatat gccgcgcggc
     4801 gtctgaatgt acttgtcgtc caccgcccgg ctgacggtcg tcacgtgcac gccgactttg
     4861 tcggcgatct gctgcatctt cagcggctcg atgaactccg ggccgtcgtc gagaaagcgg
     4921 gtctggtggt ccacgatcgc ctgcgaaacg cgggtcaacg tgttgcggcg ctgctcgatc
     4981 gactcgatca gccactgggc ggcgttgatc ttccgcttga tgtactcgcg ctcttcgggc
     5041 gtggccgtgc cgctggccag ccgctcgcga tagtgccggc tgatgtagag cgtcggcgtg
     5101 cggctctcat cgacgtgcac tttgtattgc ccgtcgtcgg agcgctccac cgtcacgtcg
     5161 ggcgtgaccg tgggtacgac ggcatcggcg aaacgcgcgc cgggcttggg attcaacttt
     5221 cgcagctcgc tccaggcctc ctggatttct tcgatcgaca agccggtgac tcgctcgatc
     5281 agcggcagcc ggttgtcgcg caggtcctcc aagtggttgg agatgagcgt atgcagctct
     5341 tcgtagaagt ccatgtccgg gtcgagctgc gccagcaggc attcgcggag gtcgcgggcg
     5401 gcgatgcccg gcgggtcgag cgattgtacg atcgacaggg ctttttcagc cgtctgcaat
     5461 tgctcctgcg cagcatcggg gggcagcaag tcgcgcaggc tggcgcgaaa atagccgccg
     5521 tccctcggat cgagcgtgct gataattcgc tcgcagaggg cgaagagctc tggcgggagc
     5581 tccatctcgc ccagttgatg gacgaggtag tcctggagcg attccggccg ctcctcgacg
     5641 ttggaaatca cgtcgtgcgc ccgctcggag tcttcctcga tgcggttcga ggagcgccgc
     5701 gtggactctt cgaaatactc gggaacgtcg cggtcgaggt tcagcaaacg ctcgaagtcg
     5761 tcggcgttgt ggtgggcctc atcgacgacg agcggcttgt cttctatcgg cttttccagc
     5821 gggttttctt gctcgaccgg ctcatcgggc aaagttggat cccgctcctg caactcgagc
     5881 gtagggttct cgaccagctc ctgctcgatc cgctcctgca attcctgcaa aggaagctgc
     5941 aagatctcca tcgactggat catccgcggc gccagttttt gcacctggag ctggcggtgt
     6001 tcgaggccga aggagagacg cataggactt actttttcga gttgcttttc agtcgggcat
     6061 caaaaccttt gtcgtccgct gagcgcgtcg gccagcactt cccgattggt gtattccagg
     6121 atgctgccgg tggtaatgcc gcgggccagc cgcgtgatct gcacaggata ttccgcgagc
     6181 atattggaga tgtgcagggc cgttccatcc ccttcgacgg ttggattggt tgccatgatg
     6241 atctcccgca cgccaccctc ccgcacacgc tcgaccagcc ggtcgatcgt cagctgttcg
     6301 gggccaaccc cgtccagcgg tgcgatccgg cccagcagca cgtggtaatg accgcggaaa
     6361 gtggcggcct gttccaaggc catcaggtcc cgcggctgct ccaccacgca gatctgcgtc
     6421 ggatcgcgtt tagggtctgt gcaaatcgcg caccgttcgc cttccgcgag gttaaagcag
     6481 atcgagcaat agtggacatt cgaacgaacg tcgcggatgg cgtcggccag ggccaaagcc
     6541 tcgttctgat gcactcgcag caggtgatac gccaatcgct cggccgattt gcgcccgatg
     6601 ccgggcagtt tggccaattc gtcgatcagg cggcccaccg actgcgtgat ctgcgtcatc
     6661 gccgcgtgcg ttccgtcttg cgtaccgttc gccgcgactc taggcctccg ccggcccaga
     6721 ttcggcaggc ggcggctcga ggaccgcgac gatctccgtg tcgaaaagtt ccgctgcctt
     6781 gcgaatcagc ggatgccgca tcagttcctg ttgccgctgc cgccggttga ttgggcgggg
     6841 cggttccggc gccggtgcgg ccgattcctc ctgcactagg gcaaagtcga tccggatgtt
     6901 gcgccccgtg attcgcgaga gcgtttgctc cagcctttgg cgtttgtccg gacgctcgca
     6961 atactgcagc gcttgagtat aggcttttcg gaaccgaacg accagtcggt ttggcccgga
     7021 aattgctaca ccgtctgccc ggccagcgaa gtcggccgtc atatctccaa tctcggcaag
     7081 ggtttgcttc catgctgaga cggccgtgct ggcatccaga accggagtcg attcgacggc
     7141 cacgacacct ggagcgtcac cgttttccga agtttttttt tgagccgaag gcggatcggc
     7201 ctcagcagtg ggggtgtgct tcgccgtggt cttggtgcca gcatccgggg gcggcgcgcc
     7261 gtcccgaagt gccgctacca ggcccggcag cgcatcgagg tcttccagct tgcacagccg
     7321 gaccaaagcc acctccacga gcgtccgcac atgcgtgctt tgacgcaagc gcgtcaatgt
     7381 atggtcgaga acctgagcca ttgcgagcag cgtttccaga ccgagcgtct cgcccagttt
     7441 tttgacctcc ggatgatcgg cggcggccgt atggagcatc aactcgggcg tgcagccgac
     7501 cagcgctgcc aggcaatcgc ggaagtagcc gagcaattgt tcggtgaacg cgcccagatc
     7561 gacgccctcg accacggcgg cttcgacttc ggccagggcc gccgccgcgt cgcgccgggc
     7621 gacgcactga gccagcgcgg tcagccgggc ggaatgagcg gtgcctagca tcgcgtgaac
     7681 gtcggccacc gtgatttgct tgccgccgaa cgagagcagt tgctccagca gcgactggct
     7741 gtcgcgcatc gagccggcgg cgcggcgggc gatgatttgc agcgcctcgg gctcggccga
     7801 aacgccctcg ctggcgcaga tgtgctgcaa ccgctccacg atcgaccgca tttcaatcgg
     7861 tgcgaaatcg aatcgctggc accgcgacaa aacagtgatc ggaatcttgt cggcttcggt
     7921 cgtgcaaaag atgaacttca catgctccgg cggctcttcc agcgtcttga gcagcgcgtt
     7981 gaacgccggc gtcgtcagca tgtggacttc gtcgatgatg taaatcttga agcgggcccg
     8041 gctggggcgg acgtttacgg tgctccgcag ctgccggatt tcgtcgatgc cgcggttgct
     8101 ggccccatcg atctcgatca catcgacgtc gtcgccgctg gcgatcccct gacagacgtc
     8161 gcactcgccg cagggctcgg tcgtcggacc gcggacgcaa ttcagagcct tggcgaagat
     8221 gcgggccgtc gaggtcttgc cgacgccgcg ggcgcccgtg aagagatagg cgtggccgac
     8281 gcgattggtc gtgatggcat tggccagggc ctgggcaacc tggccctggc cgacgagctg
     8341 cgcgaaatcc tgcggccgat accgccgggc gacgacgatg tactggccgg ctccttcccg
     8401 atctgccatg ttcgcccgcc ttccactgcc ctcgaagcgg cccccgcaca aggaaagtat
     8461 cgcttatggc tgctccggtt aaggcctgac caggttcacg gcttcccctc gcgagggccg
     8521 cttcgaacgc agtgggcgaa cgcgccgcct aggtatttgt atgcgagacg cggcgggtcg
     8581 tcaaacgggc caattgccgc cgagattacg gcgccggagc gaaatccagg agctcgagct
     8641 tgcgcatttc gactttaccg tcgcgctcga cggccaaatc catcgggctg ggcacggtgc
     8701 tcagcaagtt gacgaaatcg gcttcggact ggaacgaatg cccgccgacc gcgtaaatcc
     8761 ggtcgcgagt tttcaggccg gcgagctgcg ctgcagagct cgagatcacg tgcgtgacga
     8821 gtgccgagcc tggctcggca tcgtcaaacc gccacgaaag tccgacgcgg atcggatcgc
     8881 cgcggggcgt gaccttgatc aaaagcggtg tatcgctgcc cggccgcttg acgagaaacg
     8941 ccgtctcgcc cgctgcggcc agcagatgca gccgaaacag ctgctcgctt tcgatcggcc
     9001 ggccttgcag ctggagcaat tcgtcgcccg ctttcagccc ggccgactcg gccggcgagc
     9061 ctggcgtgag tcctgtcagc acgatcttga gcggatcggc cgactcgacc ttgaacggaa
     9121 tgccgaaccg cggcggctgc gcggcagcgg gttgctccag cgcaacgcgt ccccatgacg
     9181 attcccgccg cacggcctcg cgaaaggccg gcagctgctc ggcatcggcg agggccagca
     9241 ccgtctgaaa cgcaatttgg gtggcgccca gcagcccggc gtggttgatc cggtgggcgt
     9301 cgtcgctcgg gcggtggtag tcggcgtgca agcccgtgtg gaacatcagg aacggaatct
     9361 tctgctcgta aaatggccag tgatcgctgt cggccttcag cgaccagtcg aagtcgacgg
     9421 tcgcgcgggc ttcaccgtta gactcactga ccagccgccg aaggccggcg gcggtccgcg
     9481 agccgagcac ctccagcttg ccgtcctgca tccggccgat catgtccagg ttgatcgtgg
     9541 ccgcaacgcg ggccagcggc acggtcggcc ggctgatcca atgccgcgag ccaagcagcc
     9601 ccgcctcttc cccgtcccag caggccagca ggatcgagcg ccgcggcacc gctggcagac
     9661 gcttcagagc gtcaccgatt tccaggaggc ctgccacgcc gctggcgttg tcgtcggccc
     9721 cgttgtggat gtagccccac gggccatagc tgttggtcga gcggccgtaa cccacgtgat
     9781 cgtaatgagc gccgagtacg acgaactggc tcttgagttg cggatcgccg ccctcgacca
     9841 ggcctaggat gttgcgggag ctgccgaacg actgcaaata ggtcccgtct tctccggccg
     9901 gcttcaatcc cagcgtctcg aacgccttga gcacgtagtt gccggcggca cgaccgccgc
     9961 ggctgccggt ttcgcggccc tcgaacgtgt cgtcggccag cacatccacg tgcgacttca
    10021 gttcctcctt ggtgatcgag gccagcgcgg cttgcgcggc agcccggtcc gcggcgtccg
    10081 cgaaacgtgg ggcgagcaaa accgcaagaa tcgcagccaa gattggcgct tgcagcggcc
    10141 agcggaacgt agacatatcg agtgctcaga cagagaagag acgcgctcca tctcgactat
    10201 acgtcaattc gccgccggac gacggcggaa tgtgcccgga cttcatccgg ctagagcttt
    10261 ttccccttgc tgcggccctt caaaaccgga tcggccgcca gcggcagttg gaccggcttg
    10321 ccgctctcgg ccgacttgta aattgccagg atcaactcca ccgctcgccg cccctcgggg
    10381 ccgtcgatca gcggctgctt gccctttttg atggcattga gcacgtcctg gaactgcagc
    10441 gtgtgtccgt gatgcccgat ggccttggga tcggccgcgc cgccgccagt gtgcgtgcgg
    10501 ctgcccattt tttcgcggat ggcatcgtcg gctttcgtct ccttagcgaa cttccagacg
    10561 gccagatctt cctcgcggag cacgacacta ccggcgttgc cgctgatttc gatccgcttc
    10621 agctcgccgg gccaggtcgc cgtgctggcc tcgatcacgc cgagcgcgcc ggacgcgaat
    10681 ttgaccgtgg cgaccgcgac gtcttcgacc tcgatccgct cgtgggcgag cgtcgccgtg
    10741 tacgctgtga cttccacggc cgggcccata aaccacaaga gcagatcgac gctgtgcacg
    10801 gcctggttca tcagtgcgcc gccgccgtcg agggcccagg ttccgcgcca cgcgccgctg
    10861 tcgtaatact cctgcgtgcg aaaccacttc acgtaggcgt cgcccatcgt gagcttgccg
    10921 aaccggccct tgtcgatagc tcccttgagc aactgcgacg attcatgaaa ccgcgacgga
    10981 aagatggtgg agagcacgac gccggccttg tcggcggcgg cgatcatcgc gtcgcaccgc
    11041 ttgagcgtga tgtccagcgg cttctcgaca atgatgtgct tgccggcctt cgcagcggca
    11101 accgccggct ccatgtgcac gccgctgggc gtgcagatcg tcaccacatc gaccgacttg
    11161 tcggccagca tcgcgtccag gtcgtgatag acctggcagc cgatctccgc gccgaacttg
    11221 tcggccgcgg aggtgaacga atcgaaacag gccgcgacct gggcgccttt gatgtccgcg
    11281 acggctttgg cgtgaaaccg cgaaatcatg ccgcagccga tgatgccgaa accgatcgtc
    11341 atgtgtcagg gctcctggtg gaagccacaa gtttgttgtc agcggccgga cttcgccagg
    11401 ggtcgctaga ctttcacgcc ttcgaaaaca tggacccaca gcatccacat cgacagggcg
    11461 ttgagaccgg cgtgtacgat caggctgggg accagccgat gcgtgcgctg atacaggtag
    11521 cccatgcctg cggccaggaa cgtcaacggc acccaatcgg gtccgtgcga ccaatgcatg
    11581 agggcaaaga cgatcgcgct gatcccgatc ggcagccacg ccgcggggcc acgcagctcc
    11641 ggctgctggt cgtcaacgac ttcggcgaca aattcgggct cgctcacatc ctgcggcgaa
    11701 tcgtagggat tcaacccgcc ggtggacggc gcgaggacgg cggcgacggg tggctcgagc
    11761 gatttcggca gcggctctgc ggcgacggcc agcggaaggc cccgcggccg atcgaagagc
    11821 agctcccaca acgggccgcg atgcgtgaag gcccgctcaa gaaaaccctg caagatcacc
    11881 cgaaacatca actcctcaaa cagcggtgcg acaatcgccg ccgccacgaa caacacaatg
    11941 aagaagaccg ggttcggtgt tcctttgaag agctcgacta gcagatgcga gctgggtttc
    12001 cagaccgtga ccaggattgc ttgcagtaca tagaccggcg gggcgagcat caccaggccg
    12061 atcagcccgc ggccgatgtc gcgcggcaca tcggtcagcg agaacccaaa atcgcgcgtc
    12121 ttggctccgg tccgcagcga gatcagcggc aacccgatcg ccaggatcgc cagcgacacc
    12181 gcgacattgg caacgatgaa cgcttgccgc tggtcgaggg tgagcgtttt caggtcgctg
    12241 cccctcggaa tccaacccgt atcgcgcaat agccccgcga tggccaattg cgatcccaac
    12301 cagatgccaa cgagtgcgaa caggtcgaac agcgcccacg gcaccggtcg ccgcggcgac
    12361 cattcgacca gcggcagccg cggcgggacg cgcagcggcc ggagtacgag caacacggcg
    12421 tccgcggcgt cgcccgtgat ccagccgagg gtcaggcccg cgcgcagtcc ggcaataatc
    12481 cagcaccagc cgcaggcaaa caggccgacg acgatcaccg cggtcagccc ggcaacagcg
    12541 ggagtgagca cgttagcgtc cctgctggct gagaatgcgc cccgcggcga agacgtattc
    12601 cagtcccgaa tacaccgtcg agatcaccgc caaccagacg gaaatcgtga gcgtccaact
    12661 gagccaggga ggcagcgcag ccgacgttga accggcgagc tcgaaatgcc gcagggcgac
    12721 gaggctggcg acggctgcca cgcactgaaa gaccatcttc agcttgccgg ccatcttggc
    12781 cgagaaatcg cctccttgct cttcgatgaa gctgcgaatc accgtcacga gcatttcgcg
    12841 ccccatcacg acgacggcca tccacggcag gattccgctc cgcggaatcg ccgccaggta
    12901 gatgtacgtc ccgcaaatca ggatcttatc gacgaatgga tcgaagatcc ggccgagctt
    12961 cgtcacctgg ctgtacttgc gggcccacca gccgtcgatc caatccgtcc ccgccgccag
    13021 cacgaacacg atcgtcgccg ccaggaactg ctctagcgga atcagcacga acacgccgat
    13081 cgacagcgcc aagcggagcg ccgtgagaat gttgggaacg ttgaaaatga cgttggactg
    13141 actcacgggc gcaagccatg cgatgcgggt cggtgcagac agggcaattg gcggacttac
    13201 acctggacca ggccagccgc tgctttgctc ggtggtgcgt gcctggcctt gggccgtccc
    13261 gccgcaacgc cgatcaaatc gtagtcctgc gtagcgacga tctcgaccgg gacgatgtcg
    13321 ccggcgcgga gcttgtgctg gtcgccggtg acgaacacca ggccatcgac gtcgggcgcg
    13381 tcggcggccg tgcggcccag ccagacattc ttctcgccct ccaccggctg atccaggatc
    13441 gcgtccagcg tgcggcccac ctgcgcggcg ttccgctcga aggcgatttg ctgttgaatc
    13501 gccatcagct cttcgcggcg ctcttccatc acggcggccg gcacgtgatc aggcagcttc
    13561 gccgccggcg tatcgggctc gagcgaatac gtgaacacgc ccatccgctc gaagcggtgc
    13621 cgctcgacga aatcgcaaag ctgggcgaaa tcgtcgtctg tctcgcctgg aaatccggtg
    13681 atgaacgtcg tccgcagcgt cagatccttg attcgagatc gcaatcggcc cagcaggtct
    13741 tcggtttcag gccgcgtgac gcgccgggac atccgcttca gcattgaatc gctggcgtgc
    13801 tggagtggga tgtcgatata cggcagaatc ttgggcccat ctgcgatcgt gttgatcagc
    13861 tcgtcgtcga tatacatcgg atagaaatac atcaagcgaa tccagtcgat gccggccact
    13921 ccttccagcc gacgcagcag ctcggcgagc cggggcttgc cgtacaggtc cagcccgtaa
    13981 taggtcgtgt cctgggcgac gacgatcagc tcccgcgcgc cgtcagcggc cagctgctcc
    14041 gtctccagga cgacctcctc catgggctta ctggcgtgtt tgccgcgcat cttgggaatt
    14101 gcacagaacg tgcagagccg gtcgcagcct tcggaaatct tcagataggc aaagtgccgc
    14161 ggcgtgatcc gcagccggtc gcgatcggac agcgcccgga tcggcgcggg gcgaaagacc
    14221 gtgcgctgct cttccagccg gccaacgagg cggtcggcga cgcgcgtgat ctcttcgcgg
    14281 ccaaagacac ccacgagatg atcgatgtcc gggcgctctt cgagcaattg ctcgcgctgc
    14341 cgctcggcga ggcaccctga gacgatcacg ccgcgggtgc cgccttgttt tttcagctcc
    14401 agcatctcat cgatcgcctg gaacgattcc gcccgcgccc gctcaatgaa gccgcaggtg
    14461 ttgacgacga cgaaatcggc gcccgccggc tcgctgacca gctcgtaacc gtcgatttgc
    14521 aacagcccga gcatccgctc gctatcgacg aggttcttcg ggcagccgag gctgacaaag
    14581 gcgtaagtgc ctttggagtc cttggccgat ttcttcgaat tcttcatcac tggcctgact
    14641 gggcgattca taaactattg attttacagc acttatggca cgcttgcgag gggcgattct
    14701 cgccgctgaa aacgtgcgag ctatattggc caatcgccgc ttgtcccttc tcggccacgt
    14761 ccgtgaacga cgcactcgga atattggaag tcaccggctt gacgccagcg atggcggcgc
    14821 tcgacgcgat ggataagacc gccggcgtgc ggctgctcca gaccgagtcg aacgatttct
    14881 acggcgtggt gctgaaggtc acaggcgctc aggcgaatgt cgcggccgcc atcgcggccg
    14941 gcaaagca
//
LOCUS       DASRJB010000091         5430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2283238, whole genome shotgun sequence.
ACCESSION   DASRJB010000091 DASRJB010000000
VERSION     DASRJB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283238"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFB80_02670"
     CDS             <1..196
                     /locus_tag="VFB80_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32685.1"
                     /translation="WGGSVLNSTLHFALYQPIEGANYPAAIEMLIAAGADVSIAGPFP
                     TGNAVLDELLERHGVRPGPA"
     gene            complement(210..1589)
                     /locus_tag="VFB80_02675"
     CDS             complement(210..1589)
                     /locus_tag="VFB80_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32686.1"
                     /translation="MLHRRDAMIRLGQVGLGSLTIPGLLAAQEAAQANRRPTAKSCIL
                     VYLWGGPPQQDTFDLKPQAPDGVRSQFQPIATAVPGIQISDQLPQIARHTDKLAIIRS
                     YTHPSNQHEVGVYHTLTGKVDNTLVVPRNRRNRRDFPTMGSVVSYFKPPGDLPTAVTI
                     PRPIGHDGVIYTGTYAGFLGPQHDPLELQAPAEVQERAAHSLELPQGWDAHRLQSQVG
                     LLKTLEEYDRLNQRQAHKLQGLGQFREQAFRMLTSPSAKAAFDLTKETDQLRDRYGRN
                     EYGEAFLLARRLIESGVRLVTFVWYYVCKDGNVANVWDNHGGTGSLGGITGYQMLKEN
                     YCLPPLDQGYSALIEDLTQRGLLDETLVAMLGEFGRTPKINNTAGRDHWGACQSVVLA
                     GGGVRGGQVYGASDKTAAYPTDRPVRPEDLIATVYSALGISPEALLYDPQNRPHRASE
                     GEPLGELFG"
     gene            complement(1704..3815)
                     /locus_tag="VFB80_02680"
     CDS             complement(1704..3815)
                     /locus_tag="VFB80_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN32687.1"
                     /translation="MSETNYFWQGGRKIEIKKDDTQVTIQAADAADAHAVAERAGVEL
                     REATTAAPGLVRAEVVGDRENAVSRLREEEVVHHVYRAKDDPQQDYLITESFFVKFKP
                     DTPEDRILAYIQAEGLRVEKDLGNKTYLMRVTDDTGRNPIRAANAAATRDDVEYAEPN
                     LVRRLTRFAFIPSDPGFAQQWHLHAPADDPPDLVKGAGIFAPEAWDLTRGRRDIVIAV
                     ADDAFDLTHPDFQGQGKIVAQLNATPGNNGAIAWDSNVSPRPGDYHGTPCLGVAVAEG
                     NGTGVVGVAPGCALVAVRFPLSMEEAHFVTMFQKISALADVVSCSWGVGPADAPLSTA
                     FRDSLALLSRTGGRRGKGLVICVAAGNNNCPVKDLANTRTYRFRDSFGTIRSYSGPID
                     RWIASHPDVITVSAATSRKTRSAYSSWGREIAVCAPSDNWDDLGLTSPAGRGIMTTDN
                     EGFGSGSDFTINSRFTDRFGGTSSATPTVAGVCGLVLSRNAALSAVEVKKLLQDTADK
                     DLTIFSDTPVNQPGDFDASGRSLWFGSGKVNAFRAVQAAGGPPAEAAMLDERVTANLN
                     IPDVGAAVFSQTNVTAAGTVSDVRVSVDISHTYIGDLRVELIAPDGSSVALHNHTGGS
                     ANDIRKTYSATDTPALRSLDGKPARGTWKLRVIDTFRLDVGKLNSWRLVARLAGSPVQ
                     PTSPPATERPSRRDRARATAG"
     gene            4110..>5430
                     /locus_tag="VFB80_02685"
     CDS             4110..>5430
                     /locus_tag="VFB80_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN32688.1"
                     /translation="MSISIADFWKVLEASRLLAPEQVQQLAAEFGHIKGAGEQASGKT
                     AAEWLVTRNVLSRYQATILRAGRPGPFYYGEYKVYDRVEGGRLSGQFRAIHAPTGHPV
                     LLRFLTGPVVGDPQRWAAAAYEAQGLAGIVSPHVARIFETVDLQTFKFFVTEDLRGEA
                     LEQRLAVGRFQPAEAARIARLAAIGLAQMHQCGRTYGDVRPANVLLEPAPNHPGNVKL
                     LLDPTQPPIALDFSQQQAGSRLAQQADYLAPELATPGRAPDPLTDVYALGCTLYTMLA
                     GQPPFAGGDIAQKMARHAAEAIRPLEMWGVPQPLAQIVAYLMAKNPAVRFQSAAQAAE
                     QLAALVDPAIVYAQPPLPPATLASFEYYVRQRQSQPAEQAPPPASPPVAPGALAPAAE
                     PHPLDFRSNRRNLGALVSAAAESPSPASPAVSVTPAAAVAVKAPPRPA"
BASE COUNT          833 a         1837 c         1876 g          884 t
ORIGIN      
        1 ctggggaggc agcgtgctca actcgacgct gcacttcgcc ctctaccagc cgatcgaggg
       61 cgcgaactac ccggccgcga tcgagatgct gatcgccgcc ggagcagacg tgagcatcgc
      121 cggcccgttt cccacgggaa atgccgtact cgacgagttg ctcgagcgcc acggcgtccg
      181 acctggtcca gcctagaagc ctcgaagcgc tagccaaaca actctcccag cggctccccc
      241 tcgctggcgc ggtgggggcg gttctgcggg tcgtagagca gggcttcggg tgaaatgccc
      301 agggcggaat agaccgtggc gatcaggtct tcgggccgca cgggccggtc ggtgggatac
      361 gcggcggtct tgtcgctcgc accatagacc tgcccgccgc gaacgccgcc cccggcgagc
      421 acgaccgact ggcacgctcc ccagtggtcg cggccggcgg tgttgttgat cttcggcgtg
      481 cggccgaact cgcccagcat cgccaccagc gtctcgtcca agaggccccg ctgcgtaagg
      541 tcctcgatca gcgcggaata gccctggtcg agcggcggca ggcagtagtt ctccttgagc
      601 atctgatagc cggtgattcc gcccagcgag ccagtgccgc cgtggttgtc ccagacattc
      661 gccacgttgc cgtccttgca gacgtagtac cacacgaacg tcaccagccg cacgcccgac
      721 tcgatcagcc ggcgggccag caggaacgcc tcgccatatt cgttgcggcc gtagcggtcg
      781 cggagctggt cggtctcctt ggtcaaatcg aaagccgcct tggccgaggg gctcgtaagc
      841 atccggaagg cctgctcgcg gaactgcccc aggccttgca gcttgtgggc ctgccgctgg
      901 ttcagccggt cgtattcctc cagcgtcttc agcaggccga cttgcgactg gaggcgatgg
      961 gcgtcccagc cctgcggcaa ttccaggctg tgggccgccc gctcctgcac ttcggccggg
     1021 gcctgcagct ccagcgggtc gtgctgcggc ccgagaaagc cggcgtaggt cccggtgtag
     1081 atgacgccgt cgtgcccgat gggccgcgga atcgtcaccg ccgtcggcag gtctcccggc
     1141 ggcttgaaat acgagaccac gctgcccatc gtcggaaaat cgcggcggtt gcggcgattg
     1201 cggggcacga ccagcgtatt gtctaccttg ccggtcagcg tgtgatagac gcccacctcg
     1261 tgctggttgc tggggtgcgt gtacgagcgg atgatcgcca gcttgtcggt atgccgggcg
     1321 atttgcggca gctgatcgct gatctggatt ccgggaacgg ccgtcgcgat cggctgaaac
     1381 tggctgcgga cgccatcggg ggcctgcggc ttgagatcga acgtgtcctg ctgcggcggg
     1441 ccgccccaca ggtaaaccag gatgcaggac ttggccgtgg gccggcgatt ggcctgggcc
     1501 gcttcttgcg ccgccagcag cccgggaatc gtcagcgatc cgagcccgac ctggcccagc
     1561 cggatcatcg catcgcgccg gtgcagcatg catcgtctcc ctggcgaaaa ccgccagcga
     1621 tattggagca aacgccgcgg ggagattcca gcggaaaggc accctgaccc ctctccctct
     1681 ctcaaaggga gagggaccgg cactcacccg gcggtcgccc gtgcgcgatc ccggcggctg
     1741 ggcctttccg tcgcgggcgg cgacgtcggc tgcacgggac tgccggcgag gcgggcgacg
     1801 agccgccagg aattgagctt ccccacatcg agccggaacg tgtcgatgac gcggagcttc
     1861 caggtgccgc gggcgggttt gccgtccagc gaccgcaggg cgggagtgtc ggtcgcggaa
     1921 taggtcttcc tgatgtcgtt ggccgagccg cctgtgtggt tgtgcagggc gaccgacgag
     1981 ccatcgggcg caatcaactc gacccgcaag tcgccgatgt aggtgtgcga aatatcgacg
     2041 ctgacccgca cgtcgctgac agtgcccgca gcggtcacgt tagtctggct gaagaccgcc
     2101 gcgccgacgt cgggaatgtt caggttggcg gtgacgcgct cgtccagcat cgccgcctcg
     2161 gcgggcggac cgcctgcggc ctgcacggcg cggaaggcgt tgaccttgcc gctgccgaac
     2221 cacaagctgc ggccgctggc atcgaagtcg cccggctggt tcaccggcgt gtcgctgaag
     2281 atggtgaggt ccttgtcagc cgtatcctgg agcagcttct tgacctccac cgcgctcagc
     2341 gcggcgttgc gcgagagcac caggccgcag acgccggcga cggtcggcgt cgcgctagag
     2401 gtgccgccaa agcggtcggt gaaccgcgag ttgatcgtga agtccgagcc ggagccaaag
     2461 ccctcgttgt cggtcgtcat gatgccgcgg ccggcgggcg aggtcaggcc cagatcgtcc
     2521 cagttgtccg acggggcgca gacggcaatc tcccggcccc acgacgaata ggccgaccgc
     2581 gtcttccgcg acgtggccgc gctgaccgtg atcacgtccg gatgggaggc gatccagcga
     2641 tcgatcggcc cgctgtacga gcgaatcgtg ccgaacgagt cgcggaagcg gtaggtccgc
     2701 gtattggcca ggtccttcac cgggcagttg ttgttgccgg cggcgacgca gatgaccagc
     2761 cccttgcccc gccggccgcc ggtgcgcgac agcaaggcca gcgagtcccg gaaggccgtg
     2821 ctcagtggcg catcggccgg gccgacgccc cacgagcacg acaccacgtc ggccagggcc
     2881 gagattttct ggaacatcgt cacgaagtgc gcctcttcca tgctcagcgg gaaccgcacg
     2941 gcgaccagcg cgcagccggg agccacgccg acgacgccgg tgccgtttcc ctcggcgacg
     3001 gccacgccca ggcagggtgt gccgtgatag tcgcccggcc gcgggctgac gttgctgtcc
     3061 caggcgatcg ccccgttgtt gccgggcgtg gcgttcagtt gggccacgat cttgccctgg
     3121 ccctgaaaat ccgggtgcgt caggtcgaag gcgtcgtcgg cgacggcgat cacgatgtcg
     3181 cggcggccgc gggtcaggtc ccaggcttcg ggcgcgaaga tcccggcccc tttcaccagg
     3241 tccggcggat cgtcggccgg cgcgtgcagg tgccactgct gcgcgaagcc gggatcggac
     3301 gggatgaacg cgaaccgagt cagccgccgc accaggttgg gctcggcgta ctccacgtcg
     3361 tcacgcgtgg cggcggcgtt ggctgcgcga atcgggttgc ggccggtgtc gtcggtcacc
     3421 cgcatcaggt aggtcttgtt ccccaggtcc ttctcgaccc gcaggccctc ggcctggatg
     3481 taggccagga tgcggtcctc gggcgtgtcc ggcttgaatt tgacgaagaa gctctcggtg
     3541 atcaggtaat cttgctgcgg atcgtctttg gctcggtaga cgtggtgcac gacctcctct
     3601 tcccgcagcc gcgacacggc attctcgcgg tcgccgacga cctcagcccg gaccaggccc
     3661 ggtgcggcgg tcgtcgcctc gcggagctcg accccggccc gttcggcgac cgcgtgggca
     3721 tcggcagcgt ccgccgcctg aatggtcacc tgcgtgtcgt ccttcttaat ttcgatcttg
     3781 cgaccgccct gccagaaata gttggtctcg gacatgagcc aggccctcct gcggagtcaa
     3841 cccttcccca accaatagtc gcaatccgcg ccgcgcggcg ggcgaaaaga aaaggactag
     3901 cgcccgcgaa aatcggctct caatccaggg taagggacaa ggccgcgacg taaagcattc
     3961 atcgaacgcg acttatggcc gctgcggatc gcggcagtga tttcctttca ccaggccgga
     4021 tggggcgata gactggagtt tggttcccgc gggcgatggg ccgcgggccg cagcgaagag
     4081 gggcgtcttg gaaggggtga ttcgcggctg tgagcatctc catcgcggac ttctggaaag
     4141 tgctggaagc cagccgcttg cttgcgccgg agcaagtgca gcaacttgcc gcggagttcg
     4201 ggcacatcaa gggggcgggc gagcaggcca gcggcaagac cgctgcggag tggctcgtca
     4261 cccgcaacgt cctcagccgc tatcaggcga cgattctgcg ggccggccgc ccgggcccgt
     4321 tctattacgg cgagtacaaa gtctacgacc gcgtggaagg cgggcggctg agcggacagt
     4381 tccgggccat tcacgcgcca accggccatc cggtgctgct gcggtttttg accggcccgg
     4441 tcgtcggcga tccgcagcgc tgggcggcag cggcctacga ggcgcagggc ctggctggca
     4501 tcgtttcccc gcacgtcgcg cgcatcttcg agaccgtcga tctgcagacg ttcaagttct
     4561 ttgtcaccga agacctgcgc ggcgaggcgc tcgagcagcg gctggccgtc gggcgctttc
     4621 aaccggcgga ggccgcccgc atcgcgcggc tcgcggcgat cggcctggcc cagatgcatc
     4681 agtgcgggcg gacttacggc gacgtccgcc cggccaatgt gctgctggag cccgccccca
     4741 atcaccccgg caacgtcaag ctgctgctcg atccaacaca gccgccgata gcactcgatt
     4801 tctcgcagca gcaggcgggc agccgcctgg cgcagcaggc cgattacctc gcaccggaat
     4861 tggcgacgcc cggccgcgcg cccgatccgc tgaccgatgt ctatgccctg ggctgcacgc
     4921 tgtacacaat gctggcgggt cagccgccgt tcgccggcgg agacatcgcc cagaagatgg
     4981 cccgccatgc ggccgaggcg atccggccgc tagagatgtg gggcgtgccg cagccgctgg
     5041 cgcagatcgt ggcctacttg atggccaaga atccggcggt gcggttccag tcggccgcgc
     5101 aggcagccga gcagttggcg gcgttggtcg atccggcgat cgtctatgcc cagccgccgc
     5161 tgccgccggc cacgctggcg agcttcgagt actatgtgcg gcagaggcag tcccaaccgg
     5221 ccgagcaagc cccaccgccg gcttcaccgc cggtcgcgcc gggtgcgctg gcaccagccg
     5281 ccgagccgca tccgctcgac tttcgctcca accggcgcaa tctgggcgcg ctggtcagcg
     5341 ccgcggccga gagcccttcg cccgcaagtc ccgcggtgag cgtgacgcct gccgcggccg
     5401 tggccgtgaa ggctccgccg cggccggccg
//
LOCUS       DASRJB010000092         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3694304, whole genome shotgun sequence.
ACCESSION   DASRJB010000092 DASRJB010000000
VERSION     DASRJB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694304"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFB80_02690"
     CDS             <1..646
                     /locus_tag="VFB80_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HZN32689.1"
                     /translation="TLSPEYSFVPRWRPPFISALAAEDRCHLNGLAMVDGRPAIVTAL
                     GTTDVRDGWRADKPHGGCIIDIPSGEFVTRGLSMPHSPRWHNGQLWVLESGTGGVVLV
                     DRATGKRETVAALPGFTRGVALAGPYAFVGLSKIRPTSAMDGVPLAARRDQLKCGIGV
                     IDLRNGQTVAFLEFQTAVEEIFDVQILPGIRFPEVLGFQNDSIHHTFVVPPTAP"
     gene            799..2148
                     /locus_tag="VFB80_02695"
     CDS             799..2148
                     /locus_tag="VFB80_02695"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HZN32690.1"
                     /translation="MKSAQLFLILGTACIVVAWLVFSPGPARLKPAGPLDDLLIGEPI
                     VFQNLAVFPVSSRTPRQSDRFITLDEGLRTGKVEILERSAAENAAPSADAPPAQPAPP
                     QGQTAAEPVAQPAQEPEIGSQQPGQQSARGGGRAGNSVNELLVANHSDKPLYLMPGEI
                     IIGGDQDRTIAEEIVVAPGQKPIPISVFCVEHGRWGGRDRSEYARIAQAAASDATQAL
                     AVTDGGSPAANPDQSAAQIAMQANSGKFVGSVGSLNKQVRLAVQAGEGQSKVWDEVAN
                     QNMKNAVGGQTGTFAANYSESQAAQRLTPFLDKLQAPVTGTPNIVGVVVAIDGKVESM
                     DVFESTPLFQKLWPKLLKSYALDATSSQPAAGDTKPATVGDAGRFMRDLASAQRKSDD
                     ANGDLALSRSESDAVLAFSAHERRGPAPAAQPAAKMNDSGGGFLGSAVHSAGYSKEK"
     gene            2298..3248
                     /locus_tag="VFB80_02700"
     CDS             2298..3248
                     /locus_tag="VFB80_02700"
                     /inference="COORDINATES: protein motif:HMM:NF045440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HZN32691.1"
                     /translation="MAGTTINGPHQSIPVSGGREKFCAELFDVLWDRYRQRVSYVQTY
                     EKVIREAKATFFNDHIAFRTFAAQQPQAGISTVSRIFEALGYRAAGNYHFDDKQLSAT
                     HFQHANQQFPKLFISELKIWELPAAARGIIEKSVRSHRPAIDADVLAALAGLDKDASP
                     AAELLPQVATQFHEVPWLLPAREDVAALNKVSQYAAWVLVHGYNVNHFTSLINSHGVP
                     ALDDIEKTIAALQKAGVPMKAEIEGERGSKLRQTATEAVTIDVAVHDRGVPAKMPWTY
                     AYFELAQRDGVVDPATGKRVRFEGFLGPQATNLFEMTRVK"
     gene            3309..>3876
                     /locus_tag="VFB80_02705"
     CDS             3309..>3876
                     /locus_tag="VFB80_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN32692.1"
                     /translation="MRVLSRSGVIAAAALAAAFIPAAPLRADERAEADLARKVAALVD
                     ADEKRLLDIFLDLHRHPELGFQEVRTAALVAQELKQLGYETHTGIGKTGVVGILKNGP
                     GPVIMFRSDMDALPVLEETGLEYASRATGAAANGSLVPVMHACGHDAHVTFLIGVAKV
                     MVELKANWSGTLVLIAQPAEELIEGARAM"
BASE COUNT          705 a         1289 c         1255 g          627 t
ORIGIN      
        1 aacgcttagt cccgaataca gttttgtgcc tcgctggcga ccgccgttca tctcggcgct
       61 cgccgccgag gaccgctgcc atttgaatgg tctggcgatg gtggacggcc gaccggcgat
      121 tgtcacagcg ctcggcacca ccgacgtccg cgacggctgg cgggccgaca agccgcacgg
      181 cggctgcatc atcgatattc cgtcgggcga gttcgtgacg cgcgggctgt cgatgcctca
      241 ttcgccgcgc tggcacaacg gccaactctg ggtgctcgaa tcgggcaccg gcggcgtggt
      301 gctcgtcgat cgggccacgg gaaagcgcga aacggtggcc gcgcttcccg gcttcacccg
      361 cggcgtggct ctggccggcc cttacgcatt cgtcggcctc tccaaaatcc gccccacgtc
      421 ggcgatggac ggcgtgccgc tggccgctcg gcgcgaccag ctcaagtgcg gcatcggcgt
      481 tatcgacctc cgcaacggcc agaccgtcgc cttcctcgaa ttccagacag ccgtggagga
      541 gatcttcgac gtgcagatct tgccgggcat ccggtttccc gaagtcctcg gctttcagaa
      601 cgactcgatt caccacactt tcgtcgttcc accgactgcg ccctgagccg caccgcgcgt
      661 tacgaactcg ttacccggcc gagtgcgaaa tcaattgctc ggccgctctg cgccgcctac
      721 gatggtccga tgtggggctc gagaaacctc cgtgccggcg gccagttgcc gacttttccg
      781 tgcggagagg agattgacat gaaaagcgcc caattgtttc tgatcctggg aacggcttgc
      841 attgtggtgg cctggctggt cttttcgccc ggcccggcaa ggctcaagcc cgccggcccg
      901 ctcgacgacc tgctgattgg cgagcccatt gtctttcaaa atctggcagt attcccggtg
      961 tcgtcgcgaa ctccccgcca gtccgatcgc ttcatcacgc tcgatgaggg tctgcggacc
     1021 ggcaaggtcg aaatcctgga gcggagcgct gcggaaaacg ccgcgccttc cgcggatgcc
     1081 cctcccgcgc agccggcgcc gcctcaaggt cagacggcgg ccgaaccggt tgcccagccc
     1141 gcccaagagc ccgagatcgg cagccagcag ccggggcagc agagtgcccg cggcggcgga
     1201 cgggcaggca actccgtcaa cgagctactg gtcgcgaacc actcggacaa gccgctatac
     1261 ctgatgccgg gcgagatcat tatcggcggc gatcaggacc gcacgatcgc ggaggagatc
     1321 gtcgttgcac cgggccagaa gccgatcccg atcagcgtgt tctgcgtcga gcacggccgc
     1381 tggggcggcc gggaccggtc ggaatacgcc cggatcgcgc aagcggccgc gagcgacgcc
     1441 acgcaggccc tggccgtgac cgacggcggc tcgccagctg cgaaccccga ccagagcgcc
     1501 gcgcagatcg caatgcaggc caattccgga aagttcgtcg gcagcgtggg cagcctcaac
     1561 aagcaggtcc gcctggcggt acaggccggc gagggccaaa gtaaagtttg ggacgaagtt
     1621 gccaaccaga acatgaaaaa cgccgtcggc gggcagaccg ggacgtttgc cgccaactac
     1681 tcggaatccc aggccgcgca gcgtctgacg ccgtttctcg acaagcttca ggccccagtc
     1741 accggcacgc cgaacatcgt gggcgtggtc gtggcgatcg acggcaaagt cgagtcgatg
     1801 gacgttttcg agtccacgcc gctctttcag aagctctggc ccaagctgct gaagagctac
     1861 gccctcgacg ccaccagcag ccagccggcc gcgggcgata cgaagccggc caccgtcggc
     1921 gacgctggcc gcttcatgcg tgatctcgcc agcgctcagc gaaagagcga cgacgccaac
     1981 ggagacctgg cactctcgcg cagcgagagc gacgccgtcc tggccttttc ggctcacgaa
     2041 aggcgaggcc ccgccccggc tgcccagccc gccgccaaga tgaacgactc cggaggcgga
     2101 ttcctcggca gtgctgtcca cagtgctgga tacagcaaag agaaatgaca attggatggc
     2161 gccgcacagc ggcgcgggaa tgcgatcccg cgccgttatc tttgctagcg gataagtcgg
     2221 ctgattccgg cttcgggcaa ttggcggcga gcgtctataa tcggatagat cgcccaccca
     2281 ctcgctgtga gcaagtcatg gctggcacca cgatcaatgg cccccatcag tcgattcccg
     2341 tctccggcgg gcgagagaag ttctgtgcgg agttgttcga cgtgctctgg gaccgctatc
     2401 ggcaacgggt gtcgtacgta cagacgtacg agaaggtgat tcgcgaggcc aaggcgacgt
     2461 tcttcaacga ccacatcgcc ttccgcacgt tcgccgcgca gcagccgcag gcggggatct
     2521 cgacggtgtc gcggatcttc gaggccctgg gctaccgggc ggcggggaac tatcacttcg
     2581 acgacaagca gctctcggcc acgcactttc agcacgccaa ccagcagttt cccaagctgt
     2641 tcatctcgga gctgaagatc tgggagctgc cggcggcggc ccgcggaatc attgaaaagt
     2701 ccgtgaggag ccaccggccg gcgatcgatg ccgacgtgct ggccgcgctg gcgggcctcg
     2761 acaaggacgc ctcgccggcc gcagagctgc tgccgcaagt cgcgacccag ttccacgaag
     2821 tgccttggct cttgccggcg cgggaggacg tggcggcgct taacaaggtc agccagtacg
     2881 cggcctgggt cctcgttcac ggctacaacg tcaaccactt cacgtcgctg atcaactcgc
     2941 acggcgtgcc ggcgctcgac gacatcgaga agacgatcgc cgccctgcag aaagcgggcg
     3001 tgccgatgaa ggccgagatc gaaggcgagc gcggcagcaa gctccgccag accgcgaccg
     3061 aagcggtgac gatcgacgtc gccgtccacg accgcggcgt gccggccaag atgccttgga
     3121 cctacgccta tttcgagctc gcccagcgcg atggcgtcgt cgatcccgcc accggcaagc
     3181 gggtccgctt cgaaggcttc ctcggcccgc aagcgacgaa cctgttcgag atgacgcggg
     3241 tcaagtagca gactcaatca ctaaacacga atacctgcgt ttacttgctc ggcgaggacc
     3301 atatgcatat gcgagtgttg tccaggagtg gcgtcatcgc cgcggccgcg cttgccgcag
     3361 ccttcatccc ggctgcaccg ctgcgtgccg atgaaagagc ggaggccgat ctggcgcgga
     3421 aggtcgctgc cctcgtcgat gcggatgaga agcggctcct ggacatcttc ctggacttgc
     3481 accggcatcc ggagcttgga ttccaggaag tgcggacggc cgcgctggtg gcgcaggagc
     3541 tgaagcaact cggctacgaa acgcacacgg gcatcggcaa gacgggcgtg gtcggcatcc
     3601 tgaaaaacgg ccccggcccc gtgatcatgt tccgcagcga catggacgcc ctgcccgtgc
     3661 tcgaggaaac cggcctcgag tacgccagcc gggccacggg cgctgccgcc aacggcagcc
     3721 tcgtgccggt catgcacgcc tgcggccacg acgcgcacgt tacgttcctg atcggcgtcg
     3781 ccaaggtgat ggtggaattg aaggccaatt ggtcggggac gctcgtcctg attgcccagc
     3841 cggccgagga attgatcgaa ggcgcgcggg cgatgg
//
LOCUS       DASRJB010000093        16324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1437890, whole genome shotgun sequence.
ACCESSION   DASRJB010000093 DASRJB010000000
VERSION     DASRJB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437890"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFB80_02710"
     CDS             <1..927
                     /locus_tag="VFB80_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32693.1"
                     /translation="CRVVLGFFEAGHWPCALITTQRLLSREDRSLGNSVLQSGASIGA
                     VLTPAIVWMLLTDQPGSWRSPFVIVGIAGMLWVLPWLGMVGASRELAGAQHDSAAAGP
                     SFWVSLQELLAPDRLRMLAILLAVVIPINITWQYFRAWMPLYLQEEHGVSEGRTFWFG
                     VAFYLVADVGCLAVGAAVKLLTLRGWSTHAARAATFFACSALTSLSVAAALLPPKSPA
                     AALPLLLFISFGALGLFPTFYALSQDVSQRHQGLVTGMLGFSTWVVTSFMQEWFGTRI
                     DATKSYADGLIWAGLVPLAACAALLFWRRETK"
     gene            940..2490
                     /locus_tag="VFB80_02715"
     CDS             940..2490
                     /locus_tag="VFB80_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN32694.1"
                     /translation="MDDKPVLILGAGINGAAVARELALNGIGVVVVDTADLAFGATAY
                     SSRLIHGGLRYLEYGEFSLVKESLEERARLLTLAPQLVQPLRLFIPSENYLGGLVQSA
                     ARFVGLKFGSKPAPRGSLLVRFGLAMYDAYARDPRLPKRSLHQLGEPGTPRGLNPRRS
                     SWLSAYSDCQIVHAERIVVAFLADAQAAAQQHGQSARMLTYHRAALEQGRVVIRPVDG
                     GGEPLVLEPAAIINATGAWVDDTLARLPLPSRRLMGGTKGSHFITWHAGLRDQIGEAG
                     IYAEAADGRPVFMLPFPFGTLVGTTDLPFAGDPAAVVATEEEISYLLSVVNDLFPSIG
                     LSRGDVALHYCGVRPLPFVDAKTPAAITRRHHLVWNDAAPVPLVSLVGGKLTTCRSLA
                     EETAATVLTRLGRRVAATSRERPIPRLAFDEHLPGRDLRAAGEPAAQLPAAAARQVIR
                     DEWVTHLDDLVERRLLLHFHPGLSRPVLRELAAAMVAENKLAAAAGDAAVQATIDRLR
                     SHFGRRIE"
     gene            2573..4066
                     /gene="glpK"
                     /locus_tag="VFB80_02720"
     CDS             2573..4066
                     /gene="glpK"
                     /locus_tag="VFB80_02720"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZN32695.1"
                     /translation="MTKYILSLDQGTTSSRAILFGHDGRGVATGQEEFPQILPSPGHV
                     EHDPEAIWSSQLRSAKAAIQQAKINTADIAAIGVTNQRETTVLWDKATGKPVHNAIVW
                     QSRISSGICERLKGQGAEATFRDKTGLVLDPYFSGTKIAWLLENVSGLRSRAEKGEIL
                     FGTVDSFLIWRLTGGKVHVTDVSNASRTLLLNIKTLAWDDELLKLLSIPRAILPQVRS
                     SSEIYGETDASHFGQSVPIAGNAGDQQAATFGQACFEPGSAKNTYGTGCFMLLNIGNQ
                     PRPSQHKLLTTVGWQIGGKTTYCLEGSVFVAGAVVQWLRDGLGFITTSGEVEKLARSV
                     PDTGGVILVPAFVGLGAPYWDPYARGALLGITRGTTPAHIARAAVESIAYQSRDLLDA
                     MRSDAGLPLNVLKVDGGASANDGLMQFQADIVGATVRRPVVRETTALGAAYLAGLAVG
                     YWSDLKDIARNWALDREFTPQMAAAEREQRYATWKKGVERALAWAEP"
     gene            4089..4319
                     /locus_tag="VFB80_02725"
     CDS             4089..4319
                     /locus_tag="VFB80_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32696.1"
                     /translation="MRQWDCIVGGMAIALGVVIAASAIGNARWLMDLGRPKWLAEKIG
                     RGPARGVLAAIGLACVALGLVIVSGWRPPWAQ"
     gene            complement(4394..5644)
                     /locus_tag="VFB80_02730"
     CDS             complement(4394..5644)
                     /locus_tag="VFB80_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32697.1"
                     /translation="MRRRQFLQTSAAASFLAAAPLFVPSRAFGANERVITGHIGVGGR
                     GTDNLKGFLANAAAVCDVDQSRAAAAAKKVEEAGSKCEVFADYRRLLDRQDLDAVVIS
                     TPDHWHALTTIHACQAGKDVYCEKPLSLTIAEGRKMVEAARANKRVVQTGSQQRSTPE
                     FRRACELVRNGALGQVHTILVGIPECNHAGPPVADTDPPSGFDYDFWLGPAPQRPYNK
                     NRVHYYFRFFWDYSGGQMTNFGAHHLDIAQWALGMDNSGPVATEGTGEFHPQKWHEVT
                     EKCRVTHNYASGTKVNVGQLDPDIPVGVTFIGSQGKLFVNRGKLVAEPKELADYQLKD
                     GDVRLYESSDHYQNFLSCVKSREKPICDVEIGHRSATVCHLGNIAIRIGRKLAWDPAK
                     EQFVGDDEANTWLSRPYRAPWTLA"
     gene            5813..7315
                     /locus_tag="VFB80_02735"
     CDS             5813..7315
                     /locus_tag="VFB80_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZN32698.1"
                     /translation="MADAVKVYLAADLGASGGRVVAGIFDGQQLSLEEVYRFDNGGVA
                     AAGRLQWDLLSQWSHVQRGLRAAGKIYPGQVKSVGVDTWGVDFGLIGRNDELLGNPYH
                     YRDRRTAGIFEKAFAIVPREEIFAHTGLQFMEFNTLYQLLAMKLARSPLLDVAERLLL
                     IPDLYHWLLTGQKANEFTDASTTQFYNPKTKTWASELLTKFGLPTNILGEIAQPGTRL
                     GKLLPAVADETGLAGVEVVLPGTHDTASAVMAVPAASTPGAKPNWCYISSGTWSLMGV
                     ETPEPVISDRCYQLNFTNEGGVGGTVRLLKNIGGLWLVQECRRIWKADGHEYGWDELT
                     RRASQSPPLVSLIDPDDPSFTAPKDMPAAIRDYCRRTNQPAPDSEGAVIRCALESLAL
                     RYRMVLGMLEELIGGRLETIHIVGGGTQNRLLNQMAADATSRRVVAGPVEATAIGNLM
                     MQAVADGAVGDIAQAREIISRSFAVQQFLPKNPRPWDAAYETFQRLLEKT"
     gene            complement(7335..7562)
                     /locus_tag="VFB80_02740"
     CDS             complement(7335..7562)
                     /locus_tag="VFB80_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32699.1"
                     /translation="MKMTPREGCYYAGLIFGMIVGVLGARALGQSSIVGLIVGIVIGV
                     GCGFVSERIYTALTGAEDKNSKPPPDKQQWP"
     gene            complement(7573..8349)
                     /locus_tag="VFB80_02745"
     CDS             complement(7573..8349)
                     /locus_tag="VFB80_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32700.1"
                     /translation="MRLPRVAGSCALRLGGITAPLCRGGWWIAWAIAIGILPVMISFA
                     FGIPGHQWFSALLLAALCLGLVREDAWFRGMGLIGLTFAAHSVVVIALSTAAPQRTSA
                     ILPHANAYWQKQQTWIKTGEDPEYRLEAWVPAHLQLLAASSLFTFTSLGTVTFGQGFY
                     EVDLMNFYNAQLLARSQSLPLALLLGWHVWSVLRGLGYLVLTFEIISLSLAVWSGKRL
                     SSWRTRAGRWGAGLGLLAADGVAKFLLLDVVRQQLEINLL"
     gene            complement(8448..8576)
                     /locus_tag="VFB80_02750"
     CDS             complement(8448..8576)
                     /locus_tag="VFB80_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32701.1"
                     /translation="MSDDDEGGSGLMGWVIFILIFGVGNAILYFTTGIFIIPIPRR"
     gene            complement(8647..9252)
                     /locus_tag="VFB80_02755"
     CDS             complement(8647..9252)
                     /locus_tag="VFB80_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32702.1"
                     /translation="MTKAEMRTLRFQLQLTGLLFAVSVACAVTIVCCRGLFERWLFEK
                     HAATYDSVPKRLQETVDLLRRGEPAAEALRKMTASDQESIYQCCLAENADIRATMARS
                     LCQARPEFFVARAERTVVVGSRDQKLRAVEFLECSQCSSACQTVGELGKWARLRQRPE
                     LSQRLEAAEKKLKPFAKVEKSGQDSSSQGERGQERAKTNDA"
     gene            complement(9249..10934)
                     /locus_tag="VFB80_02760"
     CDS             complement(9249..10934)
                     /locus_tag="VFB80_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32703.1"
                     /translation="MTLAGENTPLSRAFDALLTRLSIEPRMFRALVRAFVLMDFRNQQ
                     FARSTATGPKAVISPLFWVLGQNLIVGLICAVVMFARVDAFFFALVCLGISCLMTATS
                     LVVEFNEVVLTPDDLDVVGHLPVPLRTYAAARMANLLVYVLVVLASLNLYPAIVGAGL
                     RDAPWWWLPAYSAAALVSGLVTGGIVILIYAVVMRGKDYSQSQELLAWVQVVMILVLF
                     YGGQLALRDAQQGIQMAAYRPPEFVHWLPTTWLARAVAADVRWPVQSHVGVIGGFLLA
                     AVVLSACVAAELTQLYGRLQPGSATWRPTRLRPLAPPGALSAPLATWLTRPGEERTGY
                     WLAWTMLRRDVNLTMRCWPSFGIVLAALALGYFSSQLANPFLGASTKGVLSLAAIYLL
                     APCLPPIFQHLNASREHAASWVLRSAPLANRFAFAEGIRKAVTYRLAVPVLLLLSIVF
                     IIAWRDPVAGVLHVVPAWLTVWAAGCVCQATVLARIPFSQPLVRGESFGPIALVCGAF
                     SAVGTGLAVVHCFLAPSLPGYAAYLLALTLAALIVRWMAARRLAPPRLHGAAV"
     gene            complement(10931..11785)
                     /locus_tag="VFB80_02765"
     CDS             complement(10931..11785)
                     /locus_tag="VFB80_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN32704.1"
                     /translation="MIRVEQLTKTYGEKEALRGVTFAIEPGQVCGYLGPNGAGKSTTV
                     RILTGVMPPTSGAASVAGFDVAQEALEVKRRIGYVPESAAAYSTLSVEEYLALVGALH
                     HMDPADVDQRSRRTLELFGIKDSAQSRIDTLSKGMRQKVVITAALIHDPEVLLFDEPL
                     SGLDVNAARVVKDVVRGLADRGKTVLYCSHVLDVVERLCDRVIILDHGRIVADGTPAQ
                     LKDAAQRETLESVFRSLTSSENQDEIASDLVEAVSGTPAATSAATTAREPAPRPESKK
                     PHGKGKRR"
     gene            11994..13037
                     /locus_tag="VFB80_02770"
     CDS             11994..13037
                     /locus_tag="VFB80_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32705.1"
                     /translation="MRIGLVGIGFMGWIHYLAYQKVKGIKLAAVCTRDRKRRAGDWTG
                     IQGNFGPPGEKVNLDSVAKFSQLDELLADESVDVVDLCLPPHLHEPASIAALKAGKHV
                     FVEKPMALTAAGCDRMVKAALKAGKQILVGHVLPFLPEYAAARRLIDSGKYGSVLGGH
                     FKRVISDPSWLKDFFDPEKVGGPLVDLHVHDAHFIRLLFGMPTAVTSQGRLRSDVVEY
                     CNTQFRFADPKLAVSATSGVINQQGRPFTHGFEIHLEKGTIFFDFAVMADGKNTSLPL
                     TVLTSKGKVEYPQLPPGDPMLAAFEAEIKEVGTSCASGRPSKLLGGELARDAIVLCHK
                     QSESVRKGKTVAV"
     gene            complement(13078..13932)
                     /locus_tag="VFB80_02775"
     CDS             complement(13078..13932)
                     /locus_tag="VFB80_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32706.1"
                     /translation="MAFAAPPARAQTTLAWKLAAGERFDLHVEQQTASTVTVVNKTTK
                     TTIEMTLDSTWAIESVEDGIAKIRQTIRRAQVKVQAADSPPVVYDTAAPGQPSGAEKD
                     LATAVGPLVDPDSSILLTMNERGEIAAAELSPRLAELWAAGKPAAAAKKQPAQQSLDA
                     LLKQPLVILPAKAVARGEKWETTRELTTPTGKFSQQIDYTYDGRRDVAGKSSDAISFS
                     ATLAPAAPGKFKIKEQSQTGTIHFDGEAGRMISGEQKQMLVTETPYRDATITVVVESQ
                     VSRSLTPR"
     gene            complement(14019..14708)
                     /locus_tag="VFB80_02780"
     CDS             complement(14019..14708)
                     /locus_tag="VFB80_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN32707.1"
                     /translation="MPTELTWLGHATWQLKTGEHTILVDPFLDDNPAAPAAAKNINAT
                     HILVSHGHFDHIADAAEIANRCAATVIANYEITTWLAKKHVVQNTVGMNLGGGVVVPF
                     GRVKMTIAFHSSQLPDGEYGGNPCGFLLTLKEGTKIYFACDTALFGDMEFIGNGGLDV
                     AVLPIGDLFTMGPDDAIEATRLLSPRIVIPSHYNTWPPIEQDAARWAERIKAETGARP
                     VVLKPGETFEV"
     gene            complement(14711..16144)
                     /locus_tag="VFB80_02785"
     CDS             complement(14711..16144)
                     /locus_tag="VFB80_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZN32708.1"
                     /translation="MATAPDRRLLTFDPAGVFHPLHGLQARDLAKLAPSLERAREEVL
                     VEDARHYRDGTAPPEKQPLDHGFFELPERLLADFRKDRQQSMLGHILATAERLKKLVD
                     KVVVLGIGGSYMGAKALLEACCRPYFNDLSRANRGNCPRIYFEGNNIDNDASRGLLDL
                     FGRGRADSFDKRWAIVVISKSGETLETAVAFRQFASALRESVGGDGKQLAQLIVPVTG
                     ARGRLADLAKALGCPPNETFPVPDGVGGRFSVLSAVGLVPAALMGLDVVALLEGAAAM
                     NEHFRQAPPADNVVLKYVGTAHLLEARRGANVRVLSVWSKGLEYAGLWYDQLLAESLG
                     KRELGATPLTTVNTRDLHSRAQQHQEGRRDKLFTNVIVDRWRSQPLPVGRSDRDEDKL
                     NDIAGRTLPELMSAAIQGTNEAYREDGRPTADLHLPASDEWSLGQFFQMLMLATVVEG
                     RLLGINPYGQPGVQKYKDNMKRILGRE"
BASE COUNT         2883 a         5297 c         5281 g         2863 t
ORIGIN      
        1 tgccgcgtgg tgctcggatt cttcgaggcc gggcactggc cgtgtgcact gatcacgacg
       61 cagcggctgt tatcgcgcga ggaccgctcg ctgggcaaca gcgtgctgca aagcggcgcg
      121 tcgatcggcg cggtgctgac gccggcaatt gtctggatgc tcctcaccga ccagcctgga
      181 agctggcgca gcccgttcgt catcgtggga attgccggca tgctgtgggt cctgccctgg
      241 ctgggaatgg tgggcgcgag ccgcgagctg gccggcgccc aacatgatag cgccgctgcg
      301 ggaccttcgt tctgggtttc gctacaggag ttgcttgcgc ccgaccggct gcggatgctg
      361 gcgatcctgc tggccgtggt gatccccatc aacatcacct ggcagtactt ccgcgcctgg
      421 atgccgctct acttgcagga agagcacggc gtcagcgaag ggcggacctt ctggttcggc
      481 gtggcgtttt acctggtggc ggacgtcggc tgcctggccg tgggtgcggc ggtcaaactg
      541 ctcacgctcc gcggctggag cacgcacgcg gcccgcgcgg cgacgttttt cgcctgctcc
      601 gcgctcacgt cgctgagcgt cgcggccgcg ctattgccgc cgaagtcgcc ggcggccgcg
      661 ctgccgttgc tcctattcat cagcttcggg gcgctggggc tgtttcccac gttttatgcg
      721 ctctcccagg acgtctccca gcgacaccag gggctggtga ccggaatgct cggtttcagc
      781 acgtgggtcg tgacgtcgtt tatgcaagaa tggttcggga cgcggatcga cgcgacgaaa
      841 tcgtatgccg acgggctgat ctgggcgggt ctggtgccgc tggcggcgtg cgcggcgctg
      901 ctgttctggc ggcgggagac gaagtaggaa ttcgcgacga tggacgacaa gccggtgctg
      961 atcctcggag ccggcatcaa cggcgcggcc gtggcgcggg agctggcgct caacggcatc
     1021 ggcgtggtcg tcgtcgatac ggcggacctg gcgttcggcg cgacggccta ctcatcgcgt
     1081 ctgatccacg gcggcctgcg gtatctcgag tacggcgagt tttcgctggt gaaggagtcg
     1141 ctggaggagc gagcgcggct actcacgctg gcgccgcagc tggtgcagcc gctacgcctg
     1201 ttcatcccct cggaaaacta tctgggcggc ctggtgcagt cggccgcgcg atttgtgggc
     1261 ctgaagttcg gcagcaagcc ggccccgcgc ggatcactgc tagtccgttt cggcctggcg
     1321 atgtatgacg cgtacgcgcg cgatccgcgg ttgcccaaac ggtcgctgca ccagctcggc
     1381 gaaccgggca cgccgcgggg actgaatccg cggcgttcga gctggctttc ggcgtattcc
     1441 gattgccaga tcgtgcatgc cgaacggatc gtcgtggcgt ttctggccga tgcgcaggcc
     1501 gcggcgcagc agcacggtca atccgcgcgc atgctcacgt accatcgcgc cgcgctcgag
     1561 caggggcggg tcgtcatccg gccggtcgat ggcggcggcg agccgctggt gctggaaccg
     1621 gcagcgatta tcaacgccac cggcgcctgg gtcgatgaca cgctggcccg actgccgctg
     1681 ccgtcacggc ggttgatggg agggaccaag gggagccact tcatcacctg gcacgccggc
     1741 ctgcgtgacc agatcggcga ggccggcatt tatgccgagg ccgccgacgg ccggcctgtg
     1801 ttcatgttgc cgtttccctt cggcacgttg gtggggacca ccgatctgcc gtttgcgggc
     1861 gatccagcgg cggtggtcgc gacggaggag gaaatctcct acttgctttc ggtcgtcaac
     1921 gatctgtttc cgtcgatcgg gttgtcgcgg ggcgacgtcg ctctgcacta ttgcggcgtg
     1981 cggccgctcc cctttgtcga tgccaagacg cctgccgcca tcacgcggcg gcatcatctc
     2041 gtttggaacg atgccgcgcc ggtgccgctg gtgtcgctcg tcggcggcaa actgaccact
     2101 tgccggtcgc tggcggagga aacggcggcg accgtgctaa cgcgacttgg ccgaagagtt
     2161 gccgcaactt cgcgcgagcg gccgattccg cggctggcat ttgatgagca cctgccaggc
     2221 cgcgaccttc gtgccgcagg ggagccggcg gcgcagcttc ccgccgctgc cgcgcgccag
     2281 gtcatccgcg acgaatgggt tacgcacctg gacgacctgg tcgagcggcg gctgctgctc
     2341 cacttccatc ccggcttgtc gcgcccggtg ctgcgcgagc tggccgcggc gatggtcgcg
     2401 gagaacaagc tggctgccgc cgcaggcgac gccgccgtgc aagcgacgat cgaccgactc
     2461 aggtcgcact tcggccggcg gatcgagtaa gatgctcgac aataagtagc aggcacactc
     2521 cgtgtgccgt tgctgacccc tacggcacac ggagtgtgcc tactacagat ttatgaccaa
     2581 atacatcctc tctctcgacc agggcaccac ttccagccgg gcgattcttt tcggccacga
     2641 cggccggggg gtggcgacgg ggcaggagga gtttccgcag attctgccgt cgccaggaca
     2701 tgtcgagcac gatcccgagg ccatctggtc gtcgcagttg cgttcggcca aggcagcaat
     2761 ccagcaggcg aaaatcaata ccgccgatat tgccgccatc ggcgttacca accagcgcga
     2821 aacaaccgtc ctatgggaca aagcaaccgg taagccggtc cacaacgcga ttgtctggca
     2881 aagccgcatc agcagcggca tttgcgagcg gctcaagggg cagggggccg aagcgacgtt
     2941 ccgcgacaag acggggctgg tcctcgatcc gtatttctcg gggacgaaga tcgcctggct
     3001 gctggaaaac gtcagcggcc tgcggagccg cgccgagaaa ggggaaatcc tctttggcac
     3061 cgtcgattcg ttcctcatct ggcggctgac cggcggcaaa gtccacgtca ccgatgtcag
     3121 caatgccagc cgcacgctgc tgctcaatat caagacgctg gcctgggacg atgaactgct
     3181 caagctgctg agcatcccgc gggcgattct accgcaggtc cgctcgtcga gcgaaatcta
     3241 cggcgaaacg gacgcatcgc atttcggcca gagcgtgccg atcgcgggca acgccgggga
     3301 tcagcaggcg gcgacgtttg gacaagcctg cttcgagccg ggaagtgcca agaacaccta
     3361 cggcaccggc tgcttcatgc ttctgaacat cggcaatcag ccgcggccca gccagcacaa
     3421 gctgctgacg accgtcggct ggcagatcgg cggcaagacg acttactgcc tggaaggttc
     3481 cgtgttcgtc gccggcgcgg tggtccaatg gctccgcgac ggcctggggt tcatcaccac
     3541 gtccggcgaa gtcgagaagt tggcccgcag tgttcccgac accggcggcg tcattctcgt
     3601 gccggccttc gtcggcctcg gcgcaccgta ctgggacccg tatgcccgcg gcgcgctgct
     3661 cggcatcacc cgcggtacga cgccggccca catcgcgcgg gccgcggtcg aatcgatcgc
     3721 ctaccagtcg cgcgacctcc tcgacgccat gcgcagcgac gcggggctgc cgctgaacgt
     3781 actcaaggtg gacgggggcg ccagcgccaa cgacggcctg atgcagttcc aggccgacat
     3841 cgtcggcgca acggtccggc ggccggtcgt gcgcgagaca acggcactcg gtgcggcgta
     3901 cctggccggg ctggcggtcg gctactggag cgacctcaag gatattgccc gcaactgggc
     3961 gctcgaccgc gagtttacgc cgcagatggc cgctgccgag cgcgagcagc gttatgcgac
     4021 ctggaaaaag ggggtcgagc gggcgctggc gtgggcggag ccgtaaactt gccgacagga
     4081 cgaaaccgat gcggcaatgg gactgtatcg tggggggcat ggcaatcgcg ctgggagtcg
     4141 tcattgccgc cagcgccatc ggcaacgcgc gatggctgat ggatctcggg cggcccaagt
     4201 ggctggcgga aaagatcggc cgcggtccgg cgcgcggcgt gctagccgcg atcgggctgg
     4261 cgtgcgtcgc gctgggcctg gtgatcgtca gcggttggcg tccgccgtgg gcccaataag
     4321 tactaggcac actccgtgtg ccgtcgcccg ctgaaaacga agagaacggc acacggagtg
     4381 tgcctgctac tgattacgcc agcgtccacg gcgcgcgata gggccgcgag agccaggtgt
     4441 tggcctcgtc gtcgccgacg aactgctcct tggcagggtc ccaggcgagt ttgcggccga
     4501 tccgaatggc gatgttcccc aagtgacaaa ccgtcgccga gcgatggccg atctccacgt
     4561 cgcagatcgg tttctcgcgg ctcttcacgc acgacaggaa gttctggtaa tggtcgctgc
     4621 tctcatatag ccgtacgtca ccgtctttca attggtaatc cgccaactcc ttcggctcgg
     4681 ctaccagctt gccgcggttg acgaacagtt ttccctgtga gccgataaac gtcacgccca
     4741 ccggaatgtc agggtccaac tggccgacgt tcaccttcgt gccgctggca tagttgtgcg
     4801 tcacgcggca tttctcggtc acttcgtgcc acttctgcgg atggaactcg ccggtccctt
     4861 cggtcgccac cggtccgctg ttgtccatgc caagggccca ctgggcgatg tcgagatgat
     4921 gggcgccgaa gttggtcatc tgcccgccgg aatagtccca gaaaaagcgg aagtagtagt
     4981 gcacgcgatt cttgttgtac ggtcgctgcg gcgcgggccc gagccagaag tcgtagtcga
     5041 aaccgctggg cggatcggtg tcggccaccg gcgggccggc gtggttgcac tcgggaatgc
     5101 cgacgaggat tgtgtggacc tggccgagcg ctccattgcg aacgagctcg cacgcgcggc
     5161 ggaactccgg cgtgctgcgc tgctgcgatc cggtctggac gacccgcttg ttggcgcggg
     5221 ccgcctcgac catcttccgc ccctcggcga tcgtcagcga cagcggcttc tcgcagtaca
     5281 cgtccttgcc cgcctggcag gcgtgaatgg tggtcagcgc gtgccagtgg tcgggcgtgc
     5341 tgatcacgac ggcatcgagg tcctggcggt cgaggagccg gcggtagtcg gcaaaaactt
     5401 cgcacttgct ccccgcttcc tcgacctttt tcgccgccgc tgcggctcgc gactggtcca
     5461 catcgcacac cgccgcggca ttggccagga atcccttgag gttgtcggtg ccgcgcccgc
     5521 cgacgccgat gtgaccggtg atcacccgct cgttggcccc aaaggcccgc gacgggacga
     5581 acagcggagc cgcggccaga aacgacgccg cggcggacgt ttggaggaat tgacggcgac
     5641 gcatggtgga cctcgctgcg tagatcgatc ggtgccgact caatgtacca tccgggcggc
     5701 gtgctcgcac atttgtcggc ttacgctccg cgaaagcatc ttgactctcc ccgcggcgcg
     5761 tgtcacgatc caactgcggt ctgggggacg cttctggcct tggcagtcat ctatggcgga
     5821 tgcagtaaaa gtctatctcg cggccgatct gggcgcttcc ggcggccggg tcgtggccgg
     5881 catcttcgac ggccagcagc tttcgctgga agaagtctat cgcttcgaca acggcggcgt
     5941 tgccgcggcc ggccggctgc agtgggacct cctcagccag tggagccacg tgcagcgcgg
     6001 gctgcgagcc gccggcaaaa tctatcccgg ccaggtcaaa agcgtcggcg tcgatacctg
     6061 gggagtcgat ttcggactca tcggacgcaa cgacgagctg ctcggcaatc cctaccacta
     6121 tcgcgaccgc cgcacggcag gcatcttcga gaaagccttc gccatcgtgc cgcgcgaaga
     6181 aatcttcgcg cacacgggcc tccagttcat ggagttcaac acgctctacc agctcctggc
     6241 gatgaagctg gcccgctcgc cgctgctcga cgtcgccgag cggctgcttt tgatccccga
     6301 cctctaccac tggctcctca ccggccagaa ggctaacgag tttaccgacg cttccacgac
     6361 gcagttctac aaccccaaga cgaaaacctg ggccagcgag cttttgacca agttcggctt
     6421 gccgacgaac atcctggggg aaatcgccca gccggggacg cggctcggca agctcctgcc
     6481 cgccgttgcg gacgaaaccg gcctggcagg agtcgaagtc gtccttcccg gcacgcacga
     6541 cacggccagt gccgtcatgg ccgtccccgc ggccagcacg cccggcgcga agcccaactg
     6601 gtgctacatt tccagcggca cctggtcgct gatgggtgtg gaaacccccg agccggtgat
     6661 cagcgaccgc tgctaccagc tcaacttcac caatgaaggg ggcgtgggcg gcaccgtgcg
     6721 gctgctgaag aatatcggcg gcctgtggct ggtgcaggaa tgccggcgga tctggaaagc
     6781 cgacggtcac gagtacggct gggacgagtt gacgcggcgg gccagccagt cgccgccgct
     6841 cgtcagcctg atcgaccccg acgatccgtc attcacggcc cccaaggaca tgccggcggc
     6901 catccgcgac tattgccggc ggaccaatca gccggcgccc gacagcgagg gggcggtcat
     6961 ccgctgcgcc ctcgagagcc tggcgctccg ctaccgcatg gtgctgggga tgctcgagga
     7021 gctgatcggc ggccggctgg aaacgatcca catcgtcggc ggcggcacgc agaaccgcct
     7081 cctcaaccag atggcggccg acgccacgag tcgccgcgtt gtggccggac cggtcgaagc
     7141 gacggcgatc ggcaacctga tgatgcaggc agtggccgac ggcgccgtcg gcgatatcgc
     7201 ccaggctcgc gaaattatct cccggagctt cgcggtccag cagtttctgc ccaagaaccc
     7261 gcgaccgtgg gatgcggcgt acgagacatt ccagcggctg ctcgagaaga cttgatttcg
     7321 accgccaaat ggttctacgg ccattgctgc ttgtctggcg gcggcttgga attcttatcc
     7381 tcagcaccag tgagcgccgt ataaatccgc tcgctcacaa aaccgcagcc gacaccaatc
     7441 actatcccga cgatcagacc tacaatgctc gactggccga gcgcgcgtgc gccgagcacg
     7501 cccacgatca tgccgaaaat caggccggca taataacagc cttcccgcgg ggtcatcttc
     7561 ataacgatgt cctcagagga ggttgatttc gagctgctgg cgaacgacat ccagcaacag
     7621 gaacttggcc acgccgtccg ccgccaaaag tcccagtccg gccccccagc ggccagctcg
     7681 cgtccgccag gatgacagtc gctttcccga ccatacggcc agcgagaggg agataatctc
     7741 gaacgtgagc acgagatatc ccagtcccct cagcaccgac cagacgtgcc agcctagcag
     7801 cagcgctaag ggtaggctct ggctgcgggc cagcaactgg gcgttataga agttcatcag
     7861 atccacttcg tagaatccct ggccgaaggt cacggtcccc agcgacgtga acgtgaacag
     7921 cgagctagcc gccagcaact gcaagtgggc cggcacccag gcctccaggc ggtactccgg
     7981 atcctctccg gtcttgatcc aggtctgttg cttctgccag tatgcgttgg cgtgcggcag
     8041 gatcgcactg gtgcgctgcg gcgccgctgt tgacagagcg atcacgacga ctgagtgcgc
     8101 ggcgaaagtg agaccgatca ggcccatgcc gcggaaccag gcgtcctccc ggactagacc
     8161 caagcacagc gccgccagca gcaaggccga gaaccactga tgaccaggaa tgccaaaggc
     8221 gaaggagatc atcaccggca atatgccgat ggcgatcgcc caggcgatcc accagccgcc
     8281 gcggcacaaa ggcgcggtga tgcctcccag ccgaagcgcg cagctcccgg ccactcgcgg
     8341 cagtcgcatc aacttggata ggaaagtcgg cgcaggcttg accgcgtggc gcggtgccgc
     8401 gatcggctcg cgcgccggaa gcgtcgcctg gcaccgatcg gtgtcgatca tcggcgtggg
     8461 atcggaatga tgaagatgcc tgtggtgaaa tacaggatcg cattcccaac gccaaaaatc
     8521 aggatgaaaa tcacccagcc catcaggccg ctgccccctt cgtcgtcgtc actcatggcg
     8581 agttcccttc gtcacgggtt tatggaagta ggaaaagcgg cccagccacc aggcgcgggc
     8641 cgcacgtcac gcatcgttag ttttcgcgcg ctcctggccc cgttcgcctt gggaagacga
     8701 atcttggccg gatttctcga ctttggcaaa tggtttcagc tttttttcgg cggcttccag
     8761 cctttgggat agctctggac gctgccggag gcgggcccac ttgcccaatt ccccaaccgt
     8821 ctggcaggcc gacgaacact gggaacactc caggaattcc accgcccgca gcttctggtc
     8881 gcggctgccg accacgacgg tccgctcggc ccgggccacg aaaaactccg gtctcgcctg
     8941 gcacaacgag cgggccatcg tcgcccggat gtcggcattc tccgccagac agcattgata
     9001 aatcgactct tggtcgctgg ccgtcatctt ccgaagagct tccgcggccg gttcaccccg
     9061 gcgcaaaaga tcgacagtct cctgaagcct tttcggcacg gagtcgtagg tggccgcgtg
     9121 tttttcaaac agccaccgct caaacaggcc ccggcagcag acgatcgtga cggcgcacgc
     9181 gacgctaacg gcaaacaaca gccccgtgag ctgaagctga aaacgcaggg tccgcatctc
     9241 ggccttggtc atacggcggc tccgtgtagt ctcggaggcg ccagccggcg ggcagccatc
     9301 cagcggacaa tcaatgccgc cagcgtcaga gccagcaggt atgccgcata cccaggcagc
     9361 gacggggcca aaaagcaatg gacgacggcc aacccggtgc cgacggcaga aaacgcgccg
     9421 cagacgagtg cgatggggcc gaacgattca ccgcggacga ggggctggga aaagggaatc
     9481 cgcgccagca ccgtggcctg gcagacgcag ccggcagccc ataccgtcag ccacgcagga
     9541 accacgtgca gcactccagc taccggatcc cgccaggcaa tgatgaacac gatcgatagc
     9601 agcagcagca cgggcacggc cagcctatac gtgaccgctt tgcgaatccc ttcagcaaag
     9661 gcgaaacgat tggccagcgg agcgctgcgc agcacccacg aggcagcatg ctcccggctg
     9721 gcattgaggt gctgaaagat cggcggcaga cagggcgcca acaggtaaat agcggccagc
     9781 gaaagaactc ccttggtgct tgcgcccagg aacggattgg cgagctggct gctgaagtag
     9841 cccagggcca gtgctgccag cacgatcccg aagctgggcc agcagcgcat ggtcaagttc
     9901 acatcgcggc ggagcatcgt ccaggcaagc cagtatccgg tccgttcttc acccggcctg
     9961 gtcagccacg ttgccaacgg agcgctaagt gcgccgggcg gtgcgagcgg ccgcagcctg
    10021 gtagggcgcc aggttgccga tcccggctga agccttccgt aaagttgcgt tagctcggcg
    10081 gcgacgcagg ccgaaaggac gaccgccgcc agtaagaacc ccccgatgac gccgacgtgg
    10141 gactgcaccg gccagcggac gtcagcggcc acggctcggg ccagccaggt cgtgggcagc
    10201 cagtggacga actccggcgg tcgataggcc gccatctgga ttccctgctg cgcatcccgt
    10261 agcgcgagct ggccgccgta aaacaggacc aggatcatca cgacttgcac ccaggcgagg
    10321 agttcctggg actgcgaata gtccttgccg cgcatcacga ccgcgtaaat gagaatcacg
    10381 atgccgccgg tcaccagccc ggaaaccagc gcggcggcgc tgtaggccgg cagccaccac
    10441 cacggcgcat cccgcaaacc agccccgacg atggccgggt acaaattgag cgaggccagc
    10501 accaccagca cgtagaccag caggtttgcc atccgcgccg cggcataagt tcgcagaggc
    10561 actggcaggt ggccaacgac gtccagatca tcgggcgtga gcacgacctc gttgaattcc
    10621 accaccagcg atgtggcggt catcaggcac gagatgccca ggcagacgag cgcgaagaag
    10681 aacgcatcga cgcgcgcgaa catgaccacg gcgcagatca gccccacgat gaggttctgt
    10741 cccagcaccc agaacaacgg cgagatgacc gccttgggac cggtggccgt cgagcgggcg
    10801 aactgctggt tgcgaaaatc catcagcacg aaagcccgca ccagcgcgcg gaacatccgc
    10861 ggctcgatcg acagcctggt cagcaaggcg tcgaaggcgc gggaaagggg tgtattctcg
    10921 ccggccagcg tcatcgccgc ttcccctttc catggggttt tttgctctcc ggccgcggtg
    10981 ccggctctct ggcggtcgtg gccgccgagg tcgccgcagg agtgccgctg acggcttcga
    11041 ccaggtcgct ggcgatctcg tcctggttct cgctgctggt gagcgagcgg aacaccgatt
    11101 cgagagtctc gcgctgggcc gcgtccttga gctgggccgg ggtgccgtcg gccacgatcc
    11161 gcccgtgatc gaggatgatc acccggtcgc acaagcgctc caccacatcc agcacgtgcg
    11221 agcagtacag cacggttttg ccgcggtccg ccagcccccg caccacgtcc ttgacgacac
    11281 gggccgcatt gacatccaga cccgagagcg gctcgtcaaa cagcagcact tccggatcgt
    11341 gaatcagcgc cgccgtgatg accaccttct gccgcatccc tttgctgagc gtgtcgatgc
    11401 ggctctgggc ggagtccttg atgccaaaca gctcaagcgt ccgccgcgat cgctggtcca
    11461 cgtcggccgg gtccatatga tgcagcgcgc cgaccagcgc cagatactcc tcgacgctca
    11521 gggtcgagta ggcggcggcc gattccggca cgtaaccgat ccgccgcttg acctccagcg
    11581 cctcttgcgc gacgtcaaac ccggccacgc tggctgcgcc ggatgtgggg ggcattacac
    11641 ccgtgagaat gcggacggtg gtcgacttgc ccgctccgtt gggtcccagg tagccgcaaa
    11701 cctgccccgg ctcgatggcg aacgtgacac cgcggagggc ctccttctcc ccataggtct
    11761 tcgtcaattg ctcgacccga atcatgtcgt gaacgatagc tccgcgcggc tatggcgagt
    11821 ctgatgtttg gcgctcgcgt ggcggtatcg taatgctccc gccatggaac tgcaagtttt
    11881 tccttggtac tggacgcttc gatgttgaaa ctaatgctcg cggcggataa accagggact
    11941 gcactcaagt ccttcaaggc ggcaaagcct ggcaatccct ggagagcagc accatgcgca
    12001 ttggtctggt cggcatcggc tttatgggtt ggattcacta tctcgcctac cagaaggtga
    12061 aggggatcaa gctggcagcg gtctgcacac gggatcgaaa acggcgggcc ggcgattgga
    12121 ccgggattca ggggaatttt gggccgccgg gggaaaaggt gaatctggat agcgtggcca
    12181 aattttccca gcttgacgaa ctgctcgccg acgagagcgt ggacgtcgtc gatctctgcc
    12241 ttccgccgca cctgcacgag ccagcgtcga ttgccgccct caaggctggc aagcacgtct
    12301 tcgtggaaaa gccgatggct ctcacggccg ccggctgcga ccgcatggtc aaggcagcac
    12361 tgaaggcggg caagcagatt ctggtgggac acgtcctgcc gtttctgccc gagtatgcgg
    12421 cggcccgccg gctgatcgac agcggcaaat acggcagcgt gttgggaggc catttcaaac
    12481 gcgtcatctc cgacccgtcc tggctgaagg attttttcga tccggaaaaa gtgggcgggc
    12541 cgctggtgga cctgcacgtg cacgacgcgc acttcattcg cttgctcttt ggaatgccga
    12601 cggcagtcac gagtcagggg cggctgcgga gcgacgtggt cgaatattgc aacacgcagt
    12661 tccgcttcgc tgatcccaag ctggcggtca gcgcgaccag cggggtgatc aaccagcaag
    12721 ggcgaccgtt cacacacggc ttcgaaattc acctggagaa ggggacgatc ttcttcgact
    12781 tcgcggtcat ggccgacggg aagaacacgt cgctgccgct gaccgtgctg acgagcaagg
    12841 ggaaggtgga gtatccgcag cttccgccgg gcgatccgat gctggctgcg ttcgaggcgg
    12901 agatcaagga agtcggcacc agttgcgcaa gcggccggcc ctcgaaactg ctggggggcg
    12961 agttggcccg cgacgcgatc gtgctgtgcc acaagcagtc ggagtcggtg cggaaaggga
    13021 agacggttgc agtttgagcg gcggccacgg tggtgcactc gcgccgcgag cggactacta
    13081 tcgcggcgtc aggctccgcg aaacctggct ttcgacgacg accgtgatcg tcgcgtcgcg
    13141 gtaaggcgtc tcggtgacca gcatctgctt ctgctcgccc gagatcatcc gccctgcttc
    13201 gccgtcgaag tgaatcgtgc cggtctgcga ctgctctttg attttgaact tgcctggagc
    13261 agcgggagcg agtgtggccg aaaacgagat cgcatcgctg gatttcccgg ccacgtcccg
    13321 gcggccgtcg taggtgtaat cgatctgttg tgagaatttc ccggttggag tggtcagctc
    13381 gcgcgtggtc tcccacttct cgccccgggc gaccgccttg gccggcagga tcaccagcgg
    13441 ttgtttgagc agcgcatcga gactttgctg tgcaggctgc ttttttgccg cggcggcggg
    13501 cttgccggcg gcccagagct cggccagccg cggcgaaagt tccgccgccg caatctcgcc
    13561 gcgttcgttc atcgtcagca ggatcgacga atcgggatcg accagcggcc cgacggctgt
    13621 cgccagatct ttctcggcgc cgctgggctg ccctggagca gccgtgtcgt atacgactgg
    13681 cggactatcg gccgcctgga ccttgacctg ggcgcggcgg atcgtctgcc ggattttggc
    13741 gattccgtcc tcgacgcttt ctatggccca cgtgctgtcg agcgtcatct cgatcgtcgt
    13801 tttggtggtc ttgttcacca cggtcacggt cgaggcggtc tgctgctcga cgtgcagatc
    13861 gaatcgttcc cccgccgcca gcttccaggc gagcgtcgtc tgggcccgcg ccggtggcgc
    13921 agcgaaagcc actgcggcaa atagaatcgt tattcccacc cttcgcattt ctccctcact
    13981 ctcatcgccg gctttcgcag agcgaaagcc gacattggct acacctcaaa cgtttccccc
    14041 ggcttgagca ccaccggcct ggccccagtc tcggccttga tccgctcggc ccagcgcgcc
    14101 gcgtcctgct cgatcggcgg ccaggtgttg tagtgagatg ggatcacgat ccgcggcgaa
    14161 agcagcctcg ttgcctcgat tgcgtcgtcc ggccccatcg tgaacaggtc gccgatcggc
    14221 agcaccgcca cgtccaaccc gccgttgccg atgaactcca tgtcgccgaa caaagccgtg
    14281 tcacaggcga agtagatctt cgttccctcc ttcagcgtca gcaagaagcc gcacggattg
    14341 ccgccgtact cgccgtcggg gagttgcgag ctgtgaaagg caatcgtcat cttcacccgc
    14401 ccgaatggta cgaccacgcc gccgccgagg ttcatgccga ccgtattttg cacgacgtgt
    14461 ttcttggcca gccaggtggt gatctcgtag ttggcgatca ccgtcgccgc gcagcggttg
    14521 gcgatctccg cggcatcggc gatgtggtcg aagtggccgt gcgagacgag gatgtgcgtc
    14581 gcgtttatgt tcttggccgc ggccggggcc gccgggttat cgtccaggaa cggatcgacg
    14641 aggatcgtat gctcccccgt cttgagctgc cacgtggcgt ggccgagcca ggtgagttct
    14701 gtgggcatgg ttactccctc cccaagatcc gcttcatgtt gtctttgtac ttctgcacgc
    14761 ccggctgccc gtacgggttg atgcccaaca gccgcccctc gacgacggtg gccagcatca
    14821 gcatctggaa gaactgcccc agcgaccact cgtcgctggc gggcaggtgc aggtcggccg
    14881 ttggccgccc atcctcacgg taggcctcgt tcgtcccctg gatggccgca ctcatcagct
    14941 ccggcagcgt ccgcccggcg atgtcattga gcttgtcttc gtcccggtcg ctacggccga
    15001 ccggcagggg ctgggagcgc cagcgatcga cgatgacatt ggtgaagagc ttgtcgcgcc
    15061 gtccttcctg gtgctgctgg gcccgcgaat gcagatcgcg agtgttgact gtcgtcagcg
    15121 gcgtggcccc cagctcgcgc ttgcccaggc tctcagccag caactggtcg taccacagtc
    15181 cggcgtactc cagtcccttc gaccacacgg acaacacccg cacgttcgcg ccgcggcgtg
    15241 cttccagcag atgggcagtt ccaacgtact tcagcacgac gttgtccgcc ggtggagcct
    15301 ggcgaaaatg ctcgttcatg gcggcggccc cttccagcag ggccaccaca tccagtccca
    15361 tcagcgccgc cggaaccagg cctacggccg acaggactga aaagcggccg ccgacgccgt
    15421 cgggtacggg aaacgtctcg ttcggcgggc agccgagggc tttcgccaaa tccgccagcc
    15481 ggcccctggc gccagtgaca ggcacgatga gctgcgccag ttgctttccg tcgccgccca
    15541 cgctctcacg cagggcgctg gcaaactggc gaaaagcgac tgccgtttcg agcgtttcgc
    15601 cgctcttgct gatcacgacg atcgcccacc gcttgtcgaa cgaatcggcc cggccgcggc
    15661 cgaacagatc tagcaggccg cgcgacgcat cgttgtcgat attgtttcct tcgaaataaa
    15721 tccggggaca attgccgcgg ttagcccgcg acagatcatt gaagtagggc cggcagcatg
    15781 cttccagaag cgctttagct cccatgtacg atccaccgat ccccagcacg accaccttgt
    15841 ccacgagttt cttcagccgc tcggcagtcg ccaggatgtg gcccagcatg ctctgctggc
    15901 gatccttccg aaaatcggcg agcagccgtt cggggagttc gaaaaagccg tggtcgagcg
    15961 gttgcttttc cggcggcgcg gttccgtcgc gataatgccg ggcgtcctca accagcactt
    16021 cctcgcgagc acgctccagg gacggcgcca gcttcgccaa atcgcgcgcc tggagcccat
    16081 gtagcggatg aaagacgccc gccggatcga atgtgagcag gcggcgatcg ggtgcggtgg
    16141 ccatggcagg gattcaatcg gaaacggatg aggcagagaa agaccgcgac tggtcagaat
    16201 accggagacg gagccggtgc ggaatgtgga ggtgcctttt gccctcgcct gccgactatg
    16261 atggactccg ccgaatcaag ccgaggttct catgcaactc aatcgacgcg cgctattgga
    16321 gctg
//
LOCUS       DASRJB010000094        13016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2284508, whole genome shotgun sequence.
ACCESSION   DASRJB010000094 DASRJB010000000
VERSION     DASRJB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13016
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284508"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFB80_02790"
     CDS             complement(<1..605)
                     /locus_tag="VFB80_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32709.1"
                     /translation="MVSQSKGASTRRGFTLVELLVVIAIIGVLVALLLPAVQAAREAA
                     RRSQCQNHLKQLGLGVHNFEDIYKSLPHTRMDNRYTWSVEIMPFIEQKNLFEQWNLSK
                     SYYAQTAPARLTAVPIYFCPSRRSHKLGTSGSISVPDNDIQDNTTNAPVPGALSDYAA
                     SVGSTGRDYWWDGMGSSGGDNTLLKCRGAFRMMNNWSTVTP"
     gene            685..1179
                     /locus_tag="VFB80_02795"
     CDS             685..1179
                     /locus_tag="VFB80_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN32710.1"
                     /translation="MSTWWDAPNLAADLRFGLAAKHLPRTRDWGAFVALVLLAACGCN
                     TSGLYPVEGKVVDAQGNPIPGIHESNYVMFSQTGGGLTSSMGEIAADGSFRMFTETPG
                     DGVPAGEYHVHLPRKHLDPEHTAPQVIDGRFESLENSGWTKTVEAKRNSFVLEVTPPR
                     RKGR"
     gene            complement(1186..1434)
                     /locus_tag="VFB80_02800"
     CDS             complement(1186..1434)
                     /locus_tag="VFB80_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32711.1"
                     /translation="MRILLLVATAVTLFLAAKPAEAAGRRFFKNRPPVYLTDQGAWGR
                     EFYPQYYGGIHARNMQNIGIPNGDIGIRGNGGVTPYPW"
     gene            complement(1552..2985)
                     /locus_tag="VFB80_02805"
     CDS             complement(1552..2985)
                     /locus_tag="VFB80_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN32712.1"
                     /translation="MAKLLVIDDEPAICWGLAELGKRLRHEVLTAPSAEAGLELAARE
                     RPDAVLLDVRLPGMDGVAALPKLKAHAPTAPVVIMTAHGDLSTAVEAVRQGAFDYVAK
                     PFDLKQIERLIERALAQPVAASPALIEVQADGLVGRTQAMQEVFKRIALVAPSEACVL
                     LAGASGTGKELVARAIHRYSRRSGGPFVAVNVAALSPTVAESELFGHVRGAFTGADQP
                     RTGLLVEADGGTLFLDEVADIPLAVQVKLLRALEHGEVLPVGSGRTVQTNFRLITATH
                     QDLPARVRSGEFRHDLYFRLSTFSIELPPLAQRAGDIPLLVDFFAQRLSGHSAGGAPP
                     RVSQGALEDLVSRDWHGNVRELRNAVEHALILAQGGVLLPEHLPAPLPPLDERPAAAP
                     TGAPTADPLGALRELVQRWARERLRSGEPPAALYDEFLALVEPPFLEAALKKHHGQCA
                     AAARALGIHRTTLKKKLDQYGIAGDDS"
     gene            complement(2986..4449)
                     /locus_tag="VFB80_02810"
     CDS             complement(2986..4449)
                     /locus_tag="VFB80_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN32713.1"
                     /translation="MRWPLRRQILLPMVGILLLTIGVVSALNAWLATAGLQRQLQAQL
                     ASVAQTFSRTNIPLTASVLQQTRGLTGAEFVATDLRGQPVAASDYQLVPAASGGSQRA
                     GQLDLASSIEVGSRRFIHAVLPLDRRAAGGGEFLLHVFYPEAQWREARWQALWPPLAI
                     GGAAVLLVAVAAFLVAAHVTQPIERLRSQVNAIGQGRFDPVPLPKRDDEIRELAAAVN
                     QMARQLDQYEDGLRQGERLRTLGTLGGGIAHQIRNAATGCRIALDLHQRDCPLGEANG
                     HADEPLSVAIRQLGLIETHIQRFLTLGRPPAAQKREVDLAVLVEQAIDLVRPSAAHLG
                     VALEFASPAERLVLHADCESLVQMVVNLLVNGIEATAQARLDAHRVESSGKAVRIRLH
                     QNGSLCELAVIDSGPGPAPLIQPRLFEPFATDKPGGTGLGLAVVRQIAEDHGGSVRWE
                     RRGAQTWFLVELPHSSHLAPRDEIPHAEREDYCGRST"
     gene            4645..5289
                     /locus_tag="VFB80_02815"
     CDS             4645..5289
                     /locus_tag="VFB80_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HZN32714.1"
                     /translation="MTRNRLSLLLLLAALGSAGCRSPYYADRGALFGGLTGAGVGALV
                     GEANGNAGAGAVIGSAVGAVTGAVVGEGIDADLARSRAEIEARMGRQMQGAVTVPDVI
                     AMTQAGLSDDVITTHIRASGVAQPLAVGDLIQLRNMGVRDAVINAMQQTPPRGTQPVA
                     AYPAYPAYPAGGPVIVEHHYSPWGPPVWCPPPRPHFHYHQHRPPGRVSWGVSMW"
     gene            5396..7087
                     /locus_tag="VFB80_02820"
     CDS             5396..7087
                     /locus_tag="VFB80_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32715.1"
                     /translation="MSAPTVSTPPVPFRSLTPRLPGRIIIGVALTAIVVTRVLMRLEE
                     PPRPFNDVALCNLVTMGLGFIILLTLWGWFSFRSTYPRLVRLLGLLSPIVPIVIFAPG
                     VGVLRVEEVNGDMIPTRFAFRWTLRHDQALAQVETSPAAPAIDLKTTTPDDFPQFLGP
                     ARSAWLPGPKLAREWKSQPPKLLWKKPIGAGWSAFAAVNGYAVTQEQRGESEWVACYE
                     IATGNPVWGHSIIARHENPLGGIGPRATPTIHQGCVYTLGATGMLHCLDGGTGRVIWQ
                     EDLLARFGGGMTQAEFEALVQWGYSGSPLIVDSRVVVPGGGKTAEAKNLVAFQAETGK
                     VVWEATCPRPLGGTEQIAYASPALATVAGVRQILIVNEGTASGHDAATGQCLWSHEWP
                     GHSNTDASSSQAVAVGEDRVLLSKAYSGGAELLRLTPAADSKELTLESVWKNPRVLQT
                     KFTNLVIYQDHAYGLSEGILECVSLADGQRRWKRTRGRYGNGQILGVGDLLLVISEQG
                     RLALVELNPAEFKELDTIQALDGKSWNNLCLYGNRLLLRNSQEAACYELPLEARQ"
     gene            7243..8322
                     /locus_tag="VFB80_02825"
     CDS             7243..8322
                     /locus_tag="VFB80_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZN32716.1"
                     /translation="MAAAPLAAPGPLSPPEQLRLARQIVRTEGQTLVALAERLGDEFC
                     RATDLILGSRGSVIVSGMGKAGLIGQKIAATLASTGTRSHFVHPAEAIHGDLGRVHAD
                     DVALVLSFSGQTEEVVRILPPLAQLGVPIIALTGQPASVLGRAAAVILDLGAIREACP
                     LGLAPTASTTAMLALGDALALVLSRLRQFSPADFARYHPGGSLGRKLSRVEEAMRPLA
                     QCRLACASQTLRQTLVEQSRPGRRTGAVMVTDDAGKLVGIFTDSDLARLLEANRDAAI
                     DGPLADVMTRTPTTVASGTLLTAACELLSRRKLSELPVVDADGHPLGLIDITDVLGEQ
                     VQGSKLKVQSQRKSGRRSLKVHQPG"
     gene            8420..8941
                     /locus_tag="VFB80_02830"
     CDS             8420..8941
                     /locus_tag="VFB80_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZN32717.1"
                     /translation="MDLTDRCRRIELILSDVDGVLTDGGIWYDNQGVELKGFHIRDGL
                     GIHLWRRAGFRFGVLTARTSHIVKVRAGELGIDIVRQGFEDKLPVAQEIAKSCGLTPE
                     QVCYIGDDLTDLPVIRFAGLGAAVADAVAEVRTAAALVTRLPGGRGAVRELIETILQA
                     KGRWEDAIQRYVN"
     gene            9010..12147
                     /locus_tag="VFB80_02835"
     CDS             9010..12147
                     /locus_tag="VFB80_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32718.1"
                     /translation="MLHLRRYFAALAVLSLLCGTYWLAAAPWVAAPKLGAPRPAAPAV
                     PVASSPLAKDELTRLFAANSWELNPTTKIVETDQCTLLIQDYLPTPDGLLKLTPCTLI
                     FYAKTAAGRRPIVLQAPAGALLEFDRALDVTKVSFGRLLGGKLIGEIRIFSPPSSEGA
                     RDALLMTTRDVHLGGDEIVSNYPIDFQFGDSRGRGSELKIVLQHAEAGLDGKKKRGAL
                     GGIQSLTLNKLEFARIETAGDGLLGEALPGQKKSAQSAPLEVKCRGPFTFDVPAQVAR
                     FEYQVEVERILPGSPPDKLTCDELLLTFLLRAPENAEGTTPAAKPADPMAGRLRRIVA
                     LGTPATLEAPSSGVSAKAAWMEYELARRRITLRPDNPQTKPQRVTQVSLRQYEQQFTA
                     RELQYEMPERSVADGGLRSRLGTMWAAGPGEIKLLQGRGAARQTVTARWEKELWVRPQ
                     DRNQVISLVSAASIVADPLGRFDADELHLWVREVPAEPPPAEQPPQSEQPAGKQPRTT
                     LIPDRMLAKGNVRLASPQLDAQTGELQAWFMHLPPGPEQQSPRPLVPEAPLREPVARV
                     GFQQVVPAGAPAGAIRNVIRPPNLQKFRVIGGRIQMQLAVQGRQFDLEDLTIEHQATI
                     DETRTPEPGQEPIHVAGDLLKLRHGTTPQAKVEVSGQPAEVAGRGVSLRANMINVHRG
                     ENRLWIDGPGEATLPVPAGESLPLVPGGDRGPPAPRNVTATKPAEKLHLVWQKTLDFD
                     GTLIQIEGDVHGRTRLQTVAAQRLEATLSERLDFQAPTSREQPELKRIRLSGPSGVYL
                     TNRGENEFGEQVSFDQAQFPNLTIDLDRAAGTLHADGPGWVSTVRRTAGLPGAPPAAG
                     ASAAPAPIAQPAYPPVPTNQQPLTSIHISYQRALVGRLDKREVEFQREVRTTYSPASA
                     FTDRIVIVTAADMTDKSMLMTSDKLIATEMVTPVQRWIEMQATGRTILEGQTVTVNTP
                     GRLSYSSATEFVTIEGDGRADALIYLRSAPGEQVSPQTIRSARYNIRTGQFDGEVKRL
                     EFFVPAGKGVKLPGR"
     gene            12234..>13016
                     /locus_tag="VFB80_02840"
     CDS             12234..>13016
                     /locus_tag="VFB80_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZN32719.1"
                     /translation="MLLSTICDFLDQFAPPVLAAEWDNVGLLVGDRTQKVERVLTCLT
                     ITPAVAAEAIRERADLIVTHHPLPFRPLKRLTADEPAGRLLLDLIRAGVAIHSPHTAF
                     DSAAAGINQQLAEGLGLVEIAPLVPAKDELAHVGTGRRGRFPQPQSLGQVAARLKQFL
                     KIAGLHMVGQPQQPIERVAVGCGSAGEFLADALQHNCQLLVTGETRLHTCYDAAARGV
                     ALLLSGHYASERFGVERLAAVLSTQFPQLTIWPSRDERDPLAW"
BASE COUNT         2169 a         4366 c         4411 g         2070 t
ORIGIN      
        1 tgcggcgtga ccgtgctcca gttgttcatc atgcggaacg ccccgcggca cttcagcaac
       61 gtattgtcgc cgcccgagga gcccatgccg tcccaccagt agtcgcggcc cgtcgagccg
      121 acggaagccg cgtagtcgga caaggccccc ggtactggcg cgttggtcgt gttgtcttga
      181 atgtcgttgt cgggaacgct gatcgagccg ctggtgccca gcttgtggct gcgccgcgac
      241 gggcagaaat agatcggaac cgccgtcaac cgcgccggcg cggtctgggc gtaatagctc
      301 ttgctcaggt tccactgctc gaacaggttt ttctgctcga tgaagggcat gatctcgacc
      361 gaccaggtgt agcggttgtc catccgcgtg tgcggcagcg acttgtagat gtcctcgaag
      421 ttgtggacgc ccagaccgag ctgcttgagg tggttctggc actgggaccg gcgggccgcc
      481 tcgcgcgcgg cctgaaccgc gggcaggagc agcgccacga ggacgccgat gatggcgatg
      541 acgaccaaca actcaactag cgtgaagccg cggcgggttg atgcaccctt agactgcgat
      601 accatggaat ggtccgtggc gagtgaaaga aaccccaagc taggcgggac gcaactcctt
      661 catagtattt ggcttaggtg cggaatgtca acttggtggg acgcgccgaa tctggcggcc
      721 gatcttcgct ttggcttggc ggccaagcat ctgccccgca ctcgagattg gggagcgttt
      781 gttgcccttg tgctcttggc cgcctgtggc tgcaacacgt cgggtctcta tccggtcgag
      841 ggaaaagtcg tcgatgccca gggcaatccc attcccggca ttcacgaatc caactacgtg
      901 atgttctcgc aaaccggcgg cggactcacg agcagcatgg gcgagatcgc agccgacggc
      961 tcgtttcgca tgttcaccga aacgcccggc gatggagtgc cggccggcga gtatcacgtc
     1021 cacttgccgc ggaagcatct cgatccggaa cacacggccc cgcaggtcat cgacggcagg
     1081 ttcgagagcc tcgaaaactc cggctggacg aagacggttg aagcgaagcg caacagtttc
     1141 gtcctggaag tcacgccgcc gcggcggaaa gggcgctagg gacagttacc acggatacgg
     1201 cgtcacgccg ccgttgccgc ggatgccgat gtcgccgttg gggatgccga tgttctgcat
     1261 gttgcgggcg tggatgccgc cgtagtactg cggatagaac tcgcggcccc acgcgccttg
     1321 gtcggtgaga tagacgggcg ggcgattctt gaagaaccgc cggcctgcgg cctccgcggg
     1381 ctttgcggcc aggaaaagtg taacggcggt agcgactagc aggaggatgc gcatggtagg
     1441 aagccttttc aaggtgggcg cgcggcgggc tatcaaacat agcacacaat ttggtgcgca
     1501 ttcgttcgct cgcttcgcga gcgggcgacc tcacgcggag cgtgagggct actatgagtc
     1561 gtcgccggcg atgccgtact ggtcgagctt cttcttgagc gtcgtccggt ggatgccgag
     1621 tgcgcgggcg gcggcggcgc actgcccgtg gtgctttttc agcgcggcct ccaggaaggg
     1681 cggctccacc agggcgagga actcgtcgta cagcgccgcc ggcggttcgc cgctccgcag
     1741 ccgctctcgc gcccagcgct gaacgagctc gcgcaaggcc ccgagcggat cggccgttgg
     1801 cgcgccagtc ggcgcggccg cggggcgctc gtccaagggc ggcagcggcg cgggcagatg
     1861 ctcgggcagc agaactccac cctgagcgag gatcagcgcg tgctccacgg cgttgcgcag
     1921 ctcgcggaca ttgccgtgcc agtcgcggct gacgagatcc tccagcgctc cttgcgagac
     1981 tcgcggcggc gcgccgccgg cgctgtggcc ggagagccgc tgggcgaaga aatcgaccaa
     2041 gagcggaatg tcgcccgcgc gctgagccag cggcggcagc tcgatcgaaa aggtcgagag
     2101 gcggaaatag aggtcgtggc ggaattcgcc gctccgcacc ctggccggca aatcctggtg
     2161 cgtcgcggtg atcaggcgaa agttcgtttg caccgtgcgt cccgagccga ccggcagcac
     2221 ctcgccgtgc tccagcgccc gcagcagctt gacctgcacg gccagcggaa tgtcggccac
     2281 ttcgtccaga aacagcgtcc cgccgtcggc ctcgaccagc aggcccgtcc gcggctgatc
     2341 cgccccggtg aacgctccgc gaacgtggcc aaacagctcg ctctcggcca ccgtcgggct
     2401 gagggcggct acgtttacgg cgacgaacgg tccgccgctc cgccggctgt agcgatggat
     2461 cgcccgcgcg accagctcct tgcccgttcc gctggcaccg gccagcagca cgcaggcctc
     2521 cgacggcgcg accagcgcga tccgcttgaa gacctcctgc atcgcctgcg tccggccgac
     2581 gagcccgtcg gcttgcacct cgatgagcgc cggcgacgcg gccaccggct gggccagcgc
     2641 ccgctcgatc agccgttcga tttgcttcag gtcgaacggc ttggcgacgt agtcgaacgc
     2701 gccctggcgg actgcttcca cggcggtgct gaggtcgccg tgggcggtca tgatgacgac
     2761 gggcgcggtg ggggcgtgag ccttgagctt cggcagggcc gcgacgccgt ccatgccggg
     2821 cagccgcacg tcgagcagca cggcgtcggg ccgctcgcgg gcggcgagct ccaggccggc
     2881 ctcggccgac ggcgcggtaa gcacttcgtg ccgcagccgc ttgccgagct cggccagccc
     2941 ccagcagatc gcgggttcgt cgtcgatgac gagcagcttg gccattcagg tactccggcc
     3001 acagtagtcc tcacgctccg cgtgaggaat ttcatctcgc ggagcgagat gactactatg
     3061 cggcaattcg acaaggaacc aggtctgggc gccgcgccgc tcccagcgaa ccgagccgcc
     3121 gtgatcctcg gcaatctgtc ggaccaccgc cagccccagc ccggtgccgc ccggcttgtc
     3181 cgtggcgaag ggctcgaaca aacggggctg gatgagcgga gccggtccag ggccggaatc
     3241 gatgacggcc agctcgcaga gcgatccatt ctggtgcagc cgaatgcgga cggcctttcc
     3301 gctcgactcg actcggtggg cgtcgagccg cgcctgggcc gtggcttcga tgccgttcac
     3361 cagcaggttc acaaccatct ggacgagcga ctcgcagtcc gcgtgcaaaa ccagccgctc
     3421 cgctggggac gcgaattcca gcgcgacccc caggtgcgcc gcgctcggcc gcacgagatc
     3481 gatcgcctgc tcgaccagca ccgcgaggtc cacttcgcgc ttctgagccg ctggcggccg
     3541 gcccagggtc aggaaccgct ggatgtgcgt ctcgatcagg ccgagctggc ggatcgcgac
     3601 ggacagcggc tcgtcggcgt ggccgttcgc ttcgcccagc gggcagtcgc gctggtgcag
     3661 gtccagcgcg atccggcagc cggtggcggc gttgcgaatc tggtgggcga tgcctccgcc
     3721 gagcgtgccc agcgtccgca gccgctcccc ctgccgcagg ccatcttcgt attggtcgag
     3781 ctggcgggcc atctgattca cggcggcggc caattcgcgg atttcgtcgt cgcgcttggg
     3841 cagcggcacg ggatcgaacc gcccctggcc aatggcgttg acttgcgacc tcagacgctc
     3901 gatcggctgc gtgacatgcg cggcgaccag gaacgcagcg actgccacga gcaaaactgc
     3961 cgcaccgccg atggcaagtg gcggccacag cgcctgccag cgggcctcgc gccactgggc
     4021 ctcgggataa aagacgtgca gcaggaactc gccgccgccc gccgcacgcc ggtcgagcgg
     4081 caggacggcg tggatgaacc ggcggctgcc gacctcgatg gaggaagcga ggtcgagctg
     4141 gccggctcgc tggctgcctc cggaagcggc aggcactagc tggtagtcgc tagcagctac
     4201 gggctgtccc cgcaggtcgg tagcgacgaa ctccgccccg gtcaggccgc gggtctgctg
     4261 caggacgctg gcggtcagcg gaatattggt ccgggaaaac gtttgggcga cactggccag
     4321 ctgcgcctgg agttgcctct gcaggccggc agtcgccagc caggcgttga gcgcgctgac
     4381 caccccgatg gtcagcagta ggatgccgac catgggcagc agaatctgac ggcgcagcgg
     4441 ccagcgcatc gcacggctcc gaagggtgag ctttcatcat agttgatggt gtcccgcgta
     4501 gccgaactcg ccagagttcg gatgggcagc agaaatccga attctggcga attcggctac
     4561 gtgatcctca gccggctcga tcgctcttga cggggccagc agcggcggcg aacatccgcg
     4621 gccccgcact acggaaacga caggatgacc cggaatcgac tttctctgct gctcctgctt
     4681 gccgcgcttg gttcggctgg ctgccgttcg ccctattatg ccgatcgcgg cgcgctcttc
     4741 ggcggcttga ccggcgcggg ggtcggggcg ctcgtgggcg aggcgaacgg caacgccggc
     4801 gcgggcgcgg tgatcggctc cgcagtgggc gccgtgaccg gagcggttgt cggcgaaggg
     4861 atcgacgccg atttggcccg ctcgcgggcc gagatcgaag cccggatggg ccggcagatg
     4921 caaggcgcgg tgaccgtgcc cgatgtgatc gccatgacgc aggccggcct ctccgacgac
     4981 gtgatcacga cgcacatcag ggcgagcggc gtcgcacagc cgctggccgt cggcgatttg
     5041 attcaactgc ggaacatggg cgtccgcgac gccgtgatca acgccatgca gcagacgccg
     5101 ccgcggggca cgcagccggt cgcggcctac ccggcgtatc ccgcatatcc agccggcgga
     5161 ccggtcatcg tcgagcatca ctacagtccg tggggcccgc ccgtctggtg cccgccgccg
     5221 cggccgcact tccattacca ccagcaccgt ccgccgggcc gcgtgagctg gggcgtctcc
     5281 atgtggtagc ggggaagcgc caagaaaccg aagaaccgaa gaaccaaaag gtatgttgta
     5341 gagccttgcg ttctttggtt tctttggttc ttcggttctt tgctacgata ctgttatgtc
     5401 tgcaccgacg gtctccacgc ctcctgtccc cttccgctcg ctgactcccc ggctgcccgg
     5461 gcggatcatc atcggtgtgg cgctgacggc gattgtcgtc acgcgggtgc tgatgaggct
     5521 ggaggagccg ccgcggccct tcaacgacgt cgccctgtgc aacctcgtca cgatggggtt
     5581 ggggttcatc attctgctga cgctttgggg ttggttcagc tttcgcagca cctacccgcg
     5641 cctggtgcga ttgctgggac tgctcagccc gatcgtgccg atcgtcatat tcgcgcctgg
     5701 ggtcggcgtg ctgcgtgtgg aggaggtcaa cggtgacatg atcccgacgc ggttcgcctt
     5761 tcgctggacc ctccggcacg accaggcgct ggcccaagtg gaaacgtcgc ccgccgcacc
     5821 ggcgatcgac ctgaagacga ccacgcccga tgactttccg cagttcctgg gcccggcccg
     5881 cagcgcgtgg ctgcccggac cgaagctcgc ccgcgagtgg aagtctcagc cgccgaagct
     5941 gctttggaag aagccgatcg gcgccggctg gtcggccttc gcggcggtga atgggtacgc
     6001 ggtcacgcag gagcagcgcg gcgagagcga gtgggtcgcc tgctacgaga ttgccacagg
     6061 caatccggtg tggggtcact cgatcatcgc gcggcacgag aatccgctcg gcggcatcgg
     6121 tccgcgggcc acgccgacga tccaccaggg ctgcgtctac acgctcggcg cgaccggcat
     6181 gctgcactgt ctggatggcg gtaccggcag ggtgatctgg caggaagacc tgctcgcgcg
     6241 ttttggcgga ggaatgaccc aggccgagtt cgaagccctc gtgcagtggg gctactccgg
     6301 atcgccgctg atcgttgaca gccgggtcgt cgtgccgggc ggcgggaaga cggcggaggc
     6361 caaaaacctc gtggcgttcc aggccgagac gggcaaggtc gtttgggaag cgacttgccc
     6421 ccgcccactc ggcggcaccg agcagattgc gtatgcctcg ccggccctgg cgacggtcgc
     6481 cggcgtgcgg cagattctga tcgtcaatga agggaccgcc tcgggacacg atgcggctac
     6541 cggccaatgc ctctggtccc acgaatggcc cgggcacagc aacacggacg ccagctcctc
     6601 gcaggctgtg gccgtcggcg aggaccgcgt gctgctcagc aaggcctaca gcggcggcgc
     6661 ggagctgttg cggctaacgc cggccgcgga cagcaaggag ctgacgcttg aatcggtctg
     6721 gaaaaacccg cgcgtgctgc agacgaaatt cactaacctc gtcatttatc aagatcacgc
     6781 ctatggactg tccgagggca tcttggaatg cgtctcgctg gccgacggcc agcgccgctg
     6841 gaagcgcact cgcgggcggt acggcaacgg ccagattctg ggcgtgggag acttgctgct
     6901 cgtcatctcg gagcaaggcc ggctggcact cgtcgagctg aatccagcgg aattcaagga
     6961 actcgatacg atccaggcgc tcgacggaaa aagctggaac aacctatgcc tgtatggcaa
     7021 ccggctgctc ctccgcaaca gccaggaggc cgcctgctac gagttgccgc tcgaagccag
     7081 gcaatagcgt caaagtgccg ctgctagcga ggccggacgc tgacgggttt cgcattgttc
     7141 gcctggcccg cgccgcctac aatccgcggc cgggtgcaat tgccctcgcc tgctgaagct
     7201 gcccgcaaca gcgccagcag tccgcgtcaa ggatgacgac ccatggccgc tgcgccgctc
     7261 gccgctcccg gtccgctgag cccgcccgaa cagctccgcc tggctcgcca gatcgtgcgc
     7321 accgaaggcc agacgctggt ggccctggcc gaacgcctgg gcgacgagtt ttgccgggcg
     7381 accgacctga tcctgggcag ccgcggcagc gtgatcgtca gcggcatggg caaggccggt
     7441 ctgatcggcc agaagatcgc cgccacgctg gcctccaccg gcacgcggag ccactttgtc
     7501 catccggccg aggcgatcca cggcgacttg ggccgcgtgc atgccgacga cgtggccctg
     7561 gtcttgtcgt tcagcggcca gaccgaagag gtcgtgcgga ttctgccgcc gctggcgcag
     7621 ctcggcgtac cgatcatcgc cctgacggga cagccggcga gcgtgctggg ccgggcggcg
     7681 gcggtcattc tcgacctggg agcgatccgc gaggcgtgcc cgctcggcct cgccccgacg
     7741 gccagcacga ccgcgatgct ggcgttgggg gacgcgctgg ccctggtgct gagccgcctg
     7801 cggcagttct cgccggccga tttcgcgcgg tatcatccgg gaggcagcct cggccggaag
     7861 ctgtcgcggg tggaggaagc catgcggccg ctggcgcagt gccggctggc ctgcgcgtcg
     7921 caaacgctgc ggcagacgct ggtcgaacaa agccggcccg gccgccgcac cggcgcggtg
     7981 atggtcacgg acgacgccgg caagctggtc ggcatcttca ccgacagcga cctggcccgc
     8041 ttgctggagg ccaaccgcga tgcggcgatc gacgggccgc tggcggacgt gatgacccgc
     8101 acgccgacca ccgtcgccag cggcacgctc ctgaccgccg cctgcgagct gctttcccgc
     8161 cgcaagctaa gcgaactccc cgtcgtcgat gccgacggcc acccgctcgg attgatcgat
     8221 attaccgacg tgctcggcga acaagttcaa ggttcaaagc tcaaagttca aagtcagcgc
     8281 aagagtggac gacgatcatt gaaggtacat cagcccggtt gatcggtcgg tcggtgatcg
     8341 gtaggtcggt cagtgagttg cttgaccgac ctaccgacca ccgaccaccg actgaccgac
     8401 caccgaccta ccgacgccca tggacctcac cgaccgctgc cgccgcatcg agctgatcct
     8461 gtccgacgtg gacggggtgc tgaccgacgg cggcatctgg tacgacaacc agggcgtcga
     8521 gctgaagggt tttcatatcc gcgacggcct ggggatccac ctctggcggc gggccgggtt
     8581 ccgcttcggc gtgctgaccg cccgcacgtc gcacatcgtg aaggtccggg ccggggagct
     8641 cggcatcgac atcgtccgcc agggtttcga agacaagctg ccggtggcgc aggagatcgc
     8701 caagagctgc ggattgacgc ccgagcaagt ctgttacatc ggcgacgacc tgaccgactt
     8761 gccggtgatc cgcttcgccg gcctgggcgc tgcggtggcc gacgccgtgg ccgaggtccg
     8821 caccgccgcc gccctcgtca cccgcctgcc cggcggccgc ggcgcagtcc gcgagctgat
     8881 cgaaacgatc ctgcaagcca aggggcgatg ggaggacgcc attcaacgct acgtcaacta
     8941 atgcggaatg cggaattcgg agtgagagca ggtaactccg cattccgcgc tccgcattcc
     9001 gcattttcca tgcttcacct ccgccgctat ttcgccgccc tggccgtgct gtcgctcctc
     9061 tgcggcacgt actggctggc ggctgcgccc tgggtcgctg cgccgaagct cggagctcca
     9121 cgtccggctg cgcccgcggt tccagtcgcc tcgtcgccgc tcgccaagga tgaattgacg
     9181 cggctgtttg cggccaactc ctgggagctg aaccccacga cgaagatcgt cgagaccgac
     9241 cagtgcacgc tgctgatcca ggactacctg ccgacgcccg acggcctgct caagctcacg
     9301 ccctgcacgc tgatcttcta tgccaagacg gccgccggcc gccggccaat cgtcctgcaa
     9361 gcgcccgccg gcgccttgct ggaattcgac agagccctgg acgtgaccaa ggtcagcttc
     9421 ggcaggctgc tcggcggtaa gctgatcggc gagattcgta tcttcagccc tcccagcagc
     9481 gaaggcgctc gcgacgcgct gctaatgacg acccgcgacg tccatctcgg cggcgacgag
     9541 atcgtcagca actacccgat cgattttcag ttcggcgaca gccgcggccg cggcagcgaa
     9601 ctgaagatcg tgctgcagca tgccgaagcg gggctggacg gcaagaagaa gcgcggcgca
     9661 ctgggaggca tccagtcgct gactctcaac aagctcgaat tcgcgcggat tgaaacggct
     9721 ggcgacggcc tgctcggcga ggcgctgccc ggccagaaga agtcggctca aagtgcgccg
     9781 ctggaagtca aatgccgcgg gccgttcacg ttcgacgtgc cggcccaggt ggcccggttc
     9841 gagtaccagg tcgaagtcga gcgaatcctg cccggctcgc cgcccgacaa gctgacctgc
     9901 gacgagctgc tcttgacctt cctgctccgc gcgcccgaga acgcggaggg gacgacgccg
     9961 gccgccaaac cggccgatcc gatggccggg cggctgcggc ggattgtggc cctgggcacg
    10021 ccggcgacgc tcgaagcacc ctccagcggc gtctcggcca aggcggcctg gatggaatac
    10081 gagttggctc ggcggcggat cacgctgcgg cccgacaatc cgcaaacgaa gccgcagcgc
    10141 gtgacgcagg tctcgctgcg gcagtacgag cagcaattca ccgcccgcga attgcaatat
    10201 gaaatgcccg agcgcagtgt tgctgacggc gggctgcgca gtcgcctcgg cacgatgtgg
    10261 gccgccgggc cgggcgagat caagctgctg caaggccgcg gcgcggcgcg gcagacggtc
    10321 accgcccgct gggagaaaga gctgtgggtg cggccgcaag accgaaatca ggtcatctcg
    10381 ctcgtctctg ccgccagcat cgtcgccgat ccgctgggcc gcttcgacgc cgacgaattg
    10441 cacctgtggg tccgggaagt gcccgccgaa ccgccgcccg ccgagcaacc gccccaaagc
    10501 gagcagccag ccggcaagca gccgcgaacc acgctcattc ccgaccggat gctggccaaa
    10561 ggaaacgtgc ggctagcgtc gccgcagctc gacgcccaga ccggcgagct gcaagcctgg
    10621 ttcatgcacc tgccgcccgg gccggagcaa cagtcgcctc gtccgctcgt gcctgaggcg
    10681 ccgctccgcg agccggtggc tcgcgtgggc ttccagcaag tcgttcccgc tggcgctccg
    10741 gccggcgcga tccgcaacgt catccgcccg ccgaatctgc agaagttccg cgtgatcggc
    10801 ggcaggattc agatgcagct tgcggtgcag gggcggcagt tcgacctgga ggatctgacg
    10861 atcgagcatc aggccacgat cgacgagacg cgtacgcccg aacccgggca ggagccgatt
    10921 catgtcgccg gcgacctgct gaagcttcgg cacggcacga cgccgcaggc gaaggtcgaa
    10981 gtcagcggcc agccggcgga agtcgccggc cgcggcgtat cgctgagggc gaacatgatc
    11041 aacgtccatc gcggcgagaa ccggctctgg atcgacggcc ctggcgaggc gacgctgccc
    11101 gtgccggccg gggagtcgct gccgctcgtg cccggaggag accgcgggcc gcccgctccg
    11161 cgaaacgtaa cagcaacgaa gcccgcggag aagctgcacc tggtgtggca gaaaacgctc
    11221 gacttcgatg gaacgctcat tcagattgag ggcgacgttc acggccgcac gcgcttgcag
    11281 acggtcgccg cgcagcgcct cgaggcgacg ctcagcgagc ggctggattt ccaggcgcca
    11341 acctcccgcg agcagccgga attgaaacga atccgcctca gcggtcccag cggcgtctat
    11401 ctgaccaatc gcggcgaaaa cgagttcggc gagcaggttt ccttcgacca ggcgcagttt
    11461 ccgaacctga cgatcgacct ggaccgcgcg gcgggcacgc tgcacgccga cgggcccggt
    11521 tgggtcagca cggtccgccg caccgcgggt ctgcccggag cgccgccggc cgccggagca
    11581 agcgcggcgc ctgcgccgat cgcgcagccg gcatatccgc cggttcccac gaaccagcag
    11641 ccgctgacga gtattcacat ttcgtaccag cgggcgctcg tgggccgcct ggacaaacgc
    11701 gaggtcgagt ttcagcgcga ggtgcgcacg acctattcgc cggccagcgc gttcacggat
    11761 cggatcgtca tcgtcacggc tgccgacatg acggacaaat ccatgctaat gacgagcgac
    11821 aagctgatcg ccacggaaat ggtcacgccg gtgcagcggt ggatcgagat gcaagccacc
    11881 ggccggacaa tcctcgaagg gcagaccgtt accgtgaata cgcccggccg cttgagctat
    11941 tccagcgcca cggagtttgt cacgatcgag ggggacggcc gggccgatgc gctgatttac
    12001 ctgcggtctg cgcccggcga gcaagtcagc ccgcaaacga taaggtccgc ccgctacaac
    12061 atccgcaccg gccaattcga cggcgaggtc aagcggctgg agttcttcgt ccccgccggt
    12121 aagggcgtca agctgccggg gcggtaaccc gcgagttcaa agttcaaggt tcaaggttca
    12181 aagttgattt gattgtgcta actttgaacc ttgaacttgg aacattgaac taaatgctgc
    12241 tctccaccat ctgcgatttt ctggaccagt tcgcgccgcc ggtgctggct gccgagtggg
    12301 acaacgtcgg cctgctggtt ggcgaccgga cacagaaggt ggagcgcgtt ctgacttgtc
    12361 tgacgatcac gccggcggtc gcggccgagg cgatccgcga gcgggcggac ctgatcgtca
    12421 cgcaccatcc actccccttc cggccgctga aacgacttac ggccgacgag ccggctggcc
    12481 ggctgctgct ggatctgatc cgcgcaggag tcgcaattca cagcccgcac accgcgttcg
    12541 actcggcggc tgccggcatc aaccagcagc tcgccgaggg actaggcctc gtcgagatcg
    12601 cgccgctcgt gcccgccaag gacgaactcg cacatgtcgg aaccggacgc cgcggccgct
    12661 ttccgcagcc gcagtcgctc ggccaggttg ccgcgcggct caagcagttc ctgaagatcg
    12721 cgggcctgca catggtgggc cagccgcagc agccgatcga gcgcgtggct gtcggctgcg
    12781 gcagcgcggg cgaattcctg gccgacgcgc tgcagcacaa ttgccagctg ctggtcacgg
    12841 gcgaaacgcg gctgcacact tgctacgacg ccgccgcccg cggcgttgct ctcttgctct
    12901 ctggccacta cgccagcgaa cgcttcggcg tcgagcgcct cgccgccgtc ctttcgaccc
    12961 agtttccgca gctcacgatc tggcccagcc gcgacgagcg ggatccgctg gcgtgg
//
LOCUS       DASRJB010000095        15862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1721244, whole genome shotgun sequence.
ACCESSION   DASRJB010000095 DASRJB010000000
VERSION     DASRJB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721244"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFB80_02845"
     CDS             <1..997
                     /locus_tag="VFB80_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32720.1"
                     /translation="AVKPPDDVAMLSTIPSDDGRLLAATADWLAGVGELNVEALAQLA
                     ADQEIDFATAAAYSHVCDSPKHGEFVRRIEAMEDGEPPALGSPAFRVAIVPGAFYREH
                     PHTGANGRLLAEELSRLGYQTEVLPLASFGRLANGADELRQWLSRQDGQPLVLVSLSK
                     GGSEVKLALAQGHDAFQSVIAWIDLSGILEGTALVDWLYRHPFRYLVARCILWWRKYP
                     AAGLEDLRRGTGMLLAGPLELPAGLTAIHVVGFPLKKYLCSPWAERAHARLKPLGPND
                     GGGILLGDAIARPGFVYPVWGADHYLRPAWDIRRLVARFVRYVEEEALCRTAARA"
     gene            973..2451
                     /locus_tag="VFB80_02850"
     CDS             973..2451
                     /locus_tag="VFB80_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN32721.1"
                     /translation="MSHCGQSVSGQRLRIQLLSPAGEIHRGESGIFKTALRYAPLTLT
                     TLAALVPPELDAQITIQDEGVQPLDLDFPPSLVGITAITGTSLRAYDIADRLRAKGHT
                     VVIGGVHATLLPDEAARHADAIVLSYAEKSWPQLVRDYAAGKLQSRYFARTGTRLEGV
                     PIARRDLLKKKKYATINSIEATRGCPHRCDFCAVPAAWSGIYAHRPVEEVVAELRTFE
                     GRTALFIDLSPVEDVNYAKSLYRAMIPLGIRWVGLATTRIAEDAELLDLAAKSGCKGL
                     LIGFESVSQDTLNETRKGFHKAQNYGDVVKRLHDHGIGIQGCFVFGFDSDDVSVFERT
                     VEFVDRTRIDLPRYAVMTPFPGTGYFRRLESSGRLLHKNWSLYDVEHVVFQPKQMSPE
                     RLQEGLEWAWRQSYSWRSIFARLRGAPWSILPLWLSLNFGYRYYAHHLHEKTGPVVYR
                     DQKYMEQAALELSPLAELPTATRSCSTTSAAAELLSLSVGRE"
     gene            2457..3818
                     /locus_tag="VFB80_02855"
     CDS             2457..3818
                     /locus_tag="VFB80_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN32722.1"
                     /translation="MRVTLVYPSVGRKENTPYVRAWQMEPLSMALLASLMPPDVEVSF
                     YDDRLETIPFDEPTDLVAISVETFTALRAYKIARQFRARGIPVVLGGYHVTLLPDEAQ
                     QEADAIVVGDAEPVWPQILGDARRGTLQERYQGQGRTTLSGLRPRREIFAGKKYQNIT
                     LVEFARGCNFRCDFCSITAFHHASQSHRPAREVAAEMEATGRRRFFIVDDNIVSQPAQ
                     ARELCRALIPLGISWVGQASIHIASDSELLDLMVKSGCRGVLIGMESLDPENLRDMGK
                     EWNLAGGSYADSLRRFRDHGLAVYGTFVFGYDRDDRDVILRSVEFAREQKLFLAAFNH
                     LVPFPGTPLYRRLEEQGRLLKPRWWLDPGSRVGEVTFRPKLMEPEELEEACLEARRRF
                     YSFGSIWQRMWDRQANVSSPLMLGVYLGLNLESHFDIDRRQGLRLGAGLGQWEEAHEP
                     VPV"
     gene            3799..4911
                     /locus_tag="VFB80_02860"
     CDS             3799..4911
                     /locus_tag="VFB80_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32723.1"
                     /translation="MSRFRFELATEADDADLRRVLAETPMEGRIAVAFRREPSWFVAA
                     AIDGHQRQVVACRDVESGRIVGFGCRSIRTLYVDGRPQCVGYLSSLRLLEAHRNRGLI
                     ARGYAFFRRLHNDGQTPYYLTTIADGNVTALNVLTSGRAGLPHYIPAGLYHTLAIPLV
                     QYNGVAATPGVSIRRGVPADVPAIVRFLAAHGPRRQFFPLVQEGDLRDPSGPWRGLAI
                     EDLWLAFCGAELAGVLGTWDQHAYKQSVVTGYHGTLAWLRPALNAWWSLRGYPQLPAP
                     GDPLRFRVAALPVVRDDSPAIFAALLCRAVADLAGGNCTHLLVGLHERDPLLPAVERR
                     SATRFTTHLYLVSWDDDDEIRAIISRRVPYLEPGTL"
     gene            4930..5664
                     /locus_tag="VFB80_02865"
     CDS             4930..5664
                     /locus_tag="VFB80_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32724.1"
                     /translation="MRLSARLIQRTRLTQQDRAAMLALMQTHYENVQPAVFFRDLAEK
                     RWVIVVREPTGGRLCGFSTQTILEAECDGQLIRALFSGDTIIDREHWGDPALSHVWGQ
                     FALALIDRYDDAPLYWLLLSQGYRTYRFLPLFFHEFYPRHDAATPEGLERFLDTLAER
                     RYGAAYDRKAGVVRAHAWQYRLRRGIADLSPERLRDEHVRYFAERNPGHDRGDELCCL
                     APLTRENFTRAAYRVIGQPEPALELT"
     gene            5666..7354
                     /locus_tag="VFB80_02870"
     CDS             5666..7354
                     /locus_tag="VFB80_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HZN32725.1"
                     /translation="MLARWLNAAWRWKCAGEAARFHRAMTRVAETQRDLLLSMLAANR
                     ETALGRKHVFASIKTVGDFQRQVPIATYDDYEPYIARIAAGEPGVLTSERVLLLEPTS
                     GSTAGEKLIPYTAALRQQFQRGIAAWIADLFRQRPRAARGRAYWSISPTLGPRRRTPG
                     GIPIGFDEDAAYLGLFEQWALRHLLAVPSAVAQLPDIDSVRYATLLFLLAADDLSLIS
                     VWNPTFLTSLLAALPPWADRLAADLRLGRFSPPAPVAPHVAASLARWLRPHPRRADKV
                     KSLLRDAGDDVPGEALWPRLALISCWTDAAASHFVPALARLFPQVEIQPKGLLATEGF
                     VSLPLVGQEGAALAVRSHFFEFAETLDRSRCALAHELELGGRYRVILTTGGGLYRYDL
                     GDEVEVVGFVGQCPTLRFRGKLTRTSDLVGEKLSEAHVQASLDDVTRQLNLRPTFLLL
                     TPLLDSPPHYRLYVQGVSVDALPLLTSRFHERLQENPYYRHAIAFEQLAPLDAVLLKP
                     SAAAWSLYERQCLARGQRAGNIKPAVLDRWTGWPEIFTGATAESPTPEHAPIYS"
     gene            complement(7345..8298)
                     /locus_tag="VFB80_02875"
     CDS             complement(7345..8298)
                     /locus_tag="VFB80_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN32726.1"
                     /translation="MTASPERRQRPAEMPITSTLNVALVAVVFAAATGLLWLASHAQA
                     WYLTVLAGVLFSYVLLTNYALLHEASHRNLHSDERVNYLLGVVTGLLFPMPFSMIHVT
                     HQGHHLRNRTDHEMFDMYYPTDNRLLRVLQWYSILCGLFWPVIPLGALLFAVCPRVLR
                     TRLFQRERSSSYLLGDIRNAEVNWIRLEVALIVGWFALAFWLLALDWRAVLICYACFS
                     FNWSTRQYIGHAFSKRDVIEGAWNLKHNGLMTWVLLHGEYDLNHHRRPEVPWFYLPRV
                     SPADEPRPSYVWQYWRQWLGPRAATEPAPESLQKLPLSVHE"
     gene            8442..9962
                     /locus_tag="VFB80_02880"
     CDS             8442..9962
                     /locus_tag="VFB80_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN32727.1"
                     /translation="MMTDVLARLGISRENSGVYAGGWIDRPAGEMIESINPATGEVLA
                     RVAAAGADDYARAAESSAEKFRTWQLVPPPQRGEIVRQIGQALREYKSDLGLLVTLEA
                     GKIRSEGLGEVQEMIDMADFAVGLSRQLYGLTIATERANHRMLEQWHPLGPVGVITAF
                     NFPVAVWAWNAMIAAVCGDTCLWKPSELTPLTALAVQKIVNRVLEPQGLAGLFNLVIG
                     GRDIGQQLAADARLPLVSATGSCAMGRSVATTVAGRLGRSLLELGGNNGLILAESANQ
                     DLALRAVLFAAVGTAGQRCTTLRRLIVHESIAEDFTQRLAKAYQSIQPGLPWEDGVLL
                     GPLIRQRAVEQFEAAIAAAQKQGGQVVCGGSRIKRPGFYVQPTIVRAKPEMPIVKQET
                     FAPILYVMTYKTLDEAIAIHNGVPQGLSSAIFTDGLADAERFLSPVGSDCGIANVNIG
                     TSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTCTINYGRDLPLAQGVKFDIGP"
     gene            10014..11894
                     /locus_tag="VFB80_02885"
     CDS             10014..11894
                     /locus_tag="VFB80_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN32728.1"
                     /translation="MNVRLALWVAIVAAVIVGTGLLAWTQPWRGGDADRAAAQAKGQK
                     LPPWFEDFTDKSGLNFRHWCGDGGKYFFPEVMGSGIGLIDFDRDGDLDIFVVQGMPAG
                     GAGEKKPPAGLEPAPTSRLYRRGEDGRHEDVTEEAGLIDHEPYGMGVAIGDVNNDGWP
                     DLYVSKYGQDRLYLNREGRFEDITDAAGIDNRRWGTSACFVDYDRDGWLDLFVVNYVD
                     YFPSHRCVQPNGQIDYCHPGQFTDAPAKLYRNVTGDESPKAKVQSPKSEPGGKGQGSA
                     ERGQESEVRGQGEELQVRFRDVSLEAGIDAKPGPGLGVIPGDFNGDDWIDLYVANDAK
                     ANFLWINQQGKSFRDEAIVAGAAVDIAGRPQSSMGVASGDVNGDGKSDLFMTHLDGEY
                     STLYLQLEDGVFEDRTVAAGLAAATIPYTGFGTALVDLDLDGDLDLPIANGRVRRPDE
                     MRTAADNPTEFWKQYAERNQLFLGNGDGTFTEAAPGGDPFYSRPNVARGLAIGDLDGD
                     GDLDMVTSEINGPARIYLNVAPRQGNWLSVRAVDPRHGGRDAYGALIAVVAGTQRWSR
                     DINPAYSYLSSGDPRAHFGLGKISAIDRIEVRWPDGTKESFPGGGVNREVTIQPGEGK
                     SP"
     gene            12023..13225
                     /locus_tag="VFB80_02890"
     CDS             12023..13225
                     /locus_tag="VFB80_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN32729.1"
                     /translation="MPVVSTTGMEPEVAEIIEAGRHLVEADEKSAQSWGEYGAVLRAH
                     ELGPEANVCFRNAETLDPTDYRWPYLLGVSLMTTDADAGLEAFRRAAALAEDLSHVQL
                     RLAETLINSGRYAEAAPIVDRVLKGRTLEPRAELAKARLLFEEGKLEESRTWAKKSAA
                     AAPDKRATQILLAQICRRLGNKEGEQRAQAAAAELPDIPTPWDDPDVESVKSLRRDRA
                     WQRLVGERQADSGQIAEVVELLEELTLADLAARAAIEPVRRHLLQGNFAEAEKLLREI
                     VRQHDENEKLHYQLGVLCFQQERYDAAVEEFRRAVSHKPDHVDAQYNLGHALRKMGSD
                     TEAKEAFAAAVRLSPSHARARANLAELLFDAGEPEEARQHLAVAAELAPQDPKVRELL
                     ARDRSAKD"
     gene            13274..14245
                     /locus_tag="VFB80_02895"
     CDS             13274..14245
                     /locus_tag="VFB80_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZN32730.1"
                     /translation="MNIAWVLLAYCILLIAASLAGGWLPLLTKLTHRWMQLAMSTVGG
                     FMLGVALLHLVPHSFAVLGNLDLTVGWTLAGLLAMFLLIRVFHVHAHEHGDTGHEHDH
                     EHEAKCDHPDHEHHPHPDPEHACTHDGTRERAGSGRHQFSWIGLALGLTLHSVIDGLA
                     LGAAVMADADEGNAPKGLALFGLGTFLAVVLHKPLDSLSITSLMMAGGWSRRSAVLAN
                     VVFAVICPLGAVGFVLGLNQLSSQQHVVVGCALAFAAGVFLCISLADLLPELTFHAHD
                     RLPLTAALFVGVALAWAIGYFEPPHAHGPHERGPHDHREAEHGDHGH"
     gene            14262..15089
                     /locus_tag="VFB80_02900"
     CDS             14262..15089
                     /locus_tag="VFB80_02900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN32731.1"
                     /translation="MLCIRPFADADVQFGMRLKEQAGWNQLEGDWRRMLDMQPDGCFV
                     AQFDGAAAGTCMTCIFGDVAWIAMMLVEQSLRGKGIGRALMEHALGFLDGRGTGTIRL
                     DATPMGQPLYEKLGFQAEYSLARYAGKPTGCQRPSGAHRHTRDCDELVYSLDRSVTGC
                     DRRKLLKRLIDERDCWVVGGSPLRGYLLTRAGSRATQIGPCIADDIAGPVLLNHAFDQ
                     HSESPIYIDIPLSHQPAAALAREEGLTAQRQLLRMCRGRRVDEQVERLWASSGPELG"
     gene            complement(15171..>15862)
                     /locus_tag="VFB80_02905"
     CDS             complement(15171..>15862)
                     /locus_tag="VFB80_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HZN32732.1"
                     /translation="FRGEWLLDLNGNQRWDGDDLWAQLGSQSDRPVVGDWDGDGKDDI
                     GIYGPEWPGDRQHLANEPGLPDPQNAVRMRPKNVPPDERHATKGERLMRLTSSGQERS
                     DLIDHVFEFGSGGDVPVSGDWNGDGIGSIGIFKNGQWRFDTDGDGRFSHGDEIAQFGE
                     EGDQPIVGDFNGDGIDEIGIYRAGRWIIDTNGNHQIDPEDQQLDLGDATQTPVVGDFN
                     GDGTDEPGLYK"
BASE COUNT         2737 a         5006 c         5279 g         2840 t
ORIGIN      
        1 cgcggtcaaa ccgccggatg acgttgccat gctctccacc atccctagcg acgacggccg
       61 attgctcgcg gcgacggccg attggctcgc gggcgtgggc gaattgaacg tcgaggcgct
      121 ggcacagctc gccgccgacc aggaaatcga tttcgccacg gccgcggctt atagccacgt
      181 gtgcgactca cccaagcatg gggaattcgt gcgccggatc gaggccatgg aggacggcga
      241 gccgccggcg ctgggatcgc cggcgtttcg cgtggccatc gtgccgggag cgttttatcg
      301 cgaacatccg cacactgggg cgaacgggcg attgctggcg gaagagctga gccgcctcgg
      361 atatcagacg gaggtgctgc cgctggccag ctttggccgg cttgccaacg gcgctgacga
      421 gcttcgtcag tggctgtctc ggcaagatgg ccagcccctg gtgctcgtct cgctcagcaa
      481 aggagggagc gaagtcaagc tcgcgctggc gcagggacat gatgcattcc agtccgtgat
      541 tgcctggatc gacctttccg gcatcctgga ggggacggcg ctggtcgact ggctgtaccg
      601 ccatccgttt cgttacctgg tcgcccgctg catcttgtgg tggcgaaaat atccggcggc
      661 gggactggaa gacctgcgcc gcggtacagg catgctgctg gccggcccgc tcgagctgcc
      721 agcgggactg accgcgattc acgtcgttgg ttttccgctc aaaaagtacc tctgttcccc
      781 gtgggcagag cgggcgcacg ccaggctgaa gccgctcggc cccaacgacg gcggcggcat
      841 tttgctgggg gatgcgattg cacggccagg attcgtctat cccgtgtggg gcgccgacca
      901 ctacctgcga ccggcgtggg acatccgccg gctggtcgcc cgcttcgtcc gctatgtgga
      961 agaggaggcg ctatgtcgca ctgcggccag agcgtgagcg gacagcggct acgaattcaa
     1021 ctgctctccc ccgctggcga aattcaccgc ggcgaaagcg ggattttcaa gaccgcgctc
     1081 cgttacgcgc ccctgacgct caccacgctt gcagccctgg tgcctcccga gctggatgcc
     1141 cagatcacaa tccaggacga aggggtgcag ccgctggacc tcgattttcc gcccagcctc
     1201 gtcggcatca ccgccatcac cggcacgtcg ctgcgggcct acgacattgc cgatcggctg
     1261 cgggccaagg gacacacggt cgtgatcggc ggtgtccacg ccacgctcct ccccgacgaa
     1321 gcggccaggc acgctgacgc cattgtgctg agttatgcgg aaaagtcgtg gccgcagctg
     1381 gtgcgcgatt atgccgcggg aaaattgcag tcgcggtact tcgcccgcac cggcacacgg
     1441 ttggagggag tgccgatcgc ccgccgcgat ctcctcaaga aaaagaagta cgccacgatc
     1501 aatagcatcg aggccacgcg cggctgcccc caccgctgcg acttctgcgc cgtccccgcg
     1561 gcgtggagcg gcatttacgc gcatcgaccc gtcgaagagg tcgttgccga gctgcgaaca
     1621 ttcgaggggc gcacagccct gttcatcgac ctcagcccag tcgaggacgt gaactatgcc
     1681 aagtcgctgt atcgggcgat gattccgctc gggatacggt gggtcggctt ggcgacgacg
     1741 cggattgccg aggacgcgga gcttttggac ctggcggcca aaagcggctg caaagggctg
     1801 ctgattggct tcgagtcggt cagccaggac acgctcaatg aaacccgcaa gggatttcac
     1861 aaggcgcaaa actacggcga cgtcgtcaag cggctgcacg accacggcat cggcattcaa
     1921 ggctgcttcg tgttcggctt cgacagcgac gatgtgagcg tgttcgagcg gacggtcgaa
     1981 tttgtggacc gcacgcggat tgatctgccg cgctacgccg tgatgacgcc ttttcccggc
     2041 acgggctact tccgccgcct ggaatcttcc ggacgcctgt tgcacaaaaa ctggtcgctg
     2101 tacgacgtcg agcacgtcgt gtttcagccc aagcagatga gccccgagcg gctccaggag
     2161 ggactcgagt gggcctggcg gcaaagctac tcctggcggt cgatttttgc gcgacttcgc
     2221 ggagcaccgt ggagcattct gccgctgtgg ctgtcgctca atttcggcta ccgctattac
     2281 gctcaccacc tgcacgaaaa gacggggcca gtcgtctatc gcgatcaaaa gtatatggaa
     2341 caggccgcgc tcgaattgtc gccgctggcc gaattgccga cggcgacgcg ctcgtgcagc
     2401 accacgagtg cggcggccga actcctttcc ctctccgtcg gacgggagtg acggccatgc
     2461 gggtgacgct cgtctatccg tccgtcggcc gcaaggagaa cacgccttac gtgcgcgcct
     2521 ggcagatgga gccgctctcc atggccttgc tggcgagcct gatgccgccg gacgtggaag
     2581 tcagcttcta cgacgaccgc ctggagacga ttcccttcga cgaaccgacc gacctggtgg
     2641 cgatcagcgt ggaaacgttc accgccctgc gggcctacaa gatcgctcgg caattccgcg
     2701 cccggggcat tcccgtcgtg ctcggtggct atcacgtcac cttgctcccc gacgaggcgc
     2761 agcaggaggc ggacgcgatc gtcgttgggg atgccgagcc ggtctggccg cagattcttg
     2821 gcgatgcccg ccgcggcaca ctccaagagc gctaccaggg acaaggccgc acgaccctca
     2881 gcggcctgcg cccgcggcgc gagatttttg ccggcaagaa gtaccagaac atcacgctgg
     2941 tggaattcgc ccgcggctgc aactttcgct gcgacttctg tagcatcacc gcgtttcacc
     3001 acgccagcca gagccataga ccagcccgcg aagtggctgc cgagatggag gccaccggcc
     3061 ggcggcggtt cttcatcgtg gacgacaaca tcgtcagcca gccggcccag gcccgcgaat
     3121 tgtgccgggc gctgatcccg ctgggcatca gttgggtcgg ccaggcgagc attcatatcg
     3181 cgagcgacag cgagctgctc gacctgatgg tgaaaagtgg ctgccgtggc gtgctgattg
     3241 gcatggagtc gctcgatccg gaaaatctcc gcgacatggg caaggaatgg aacctcgccg
     3301 gcggaagcta cgccgacagc ttgcggcgct ttcgcgacca cggcctggct gtatatggca
     3361 cgttcgtctt cggctatgac cgcgacgacc gtgacgtgat tctccgcagc gtcgaatttg
     3421 cacgggaaca gaaattgttc ctggccgcat tcaaccatct ggttccgttc cccggcacgc
     3481 cgctctaccg ccggttggaa gagcaaggcc ggctgctcaa gccgcgttgg tggctcgatc
     3541 cgggcagccg cgtcggcgag gtgacgtttc gccccaagct gatggagccg gaagaactcg
     3601 aagaagcctg cctggaagcc cggcggcggt tctatagctt cggctcgatc tggcagcgga
     3661 tgtgggatcg ccaggccaac gtcagcagcc cgctcatgct cggcgtctac ctgggcctga
     3721 acctggaatc gcatttcgac atcgaccggc ggcaagggct gcggctggga gctgggcttg
     3781 gccagtggga ggaggcacat gagccggttc cggtttgagc tggccaccga ggccgacgac
     3841 gccgacctcc gccgcgtgct ggccgaaacg ccgatggagg ggcggatcgc ggtcgccttt
     3901 cgccgcgagc cgagctggtt tgtcgcggcg gccattgacg gccaccagcg gcaggtcgtt
     3961 gcctgccgcg acgtcgagtc tgggcgcatc gtcggctttg gctgccgctc gattcgcacg
     4021 ctgtacgtcg atggccggcc gcaatgcgtg ggctacctca gcagcctgcg gctcctggag
     4081 gcgcaccgca atcggggcct gattgcccgc ggttatgcgt tctttcgccg gctgcacaac
     4141 gatggccaga cgccgtatta cctcaccacg attgccgacg ggaacgtcac ggcgctgaac
     4201 gtgctcacct cgggccgcgc gggactgccg cattacatcc ccgccggcct gtatcacacg
     4261 ctggccatcc cgctggtgca gtacaacggc gtcgccgcga cacccggcgt ttcgattcgc
     4321 cgcggggtgc ccgccgatgt gccggccatc gtgcgattcc ttgccgcgca tggcccgcga
     4381 agacagttct ttccgcttgt gcaggaaggt gacctccgcg atccgtccgg accgtggcgc
     4441 ggcctggcga tcgaggactt gtggctggcc ttttgcggtg ccgaactggc gggcgtgctc
     4501 ggcacttggg atcagcatgc gtataagcag agcgtcgtga ccggctatca cgggacgctc
     4561 gcctggctgc ggccggctct taacgcctgg tggtcgctgc gcggctaccc tcagctcccg
     4621 gccccgggcg acccactgcg gtttcgcgtg gcggcgctgc cggtcgtgcg cgacgactcc
     4681 cccgcgatct tcgcggcgct cttgtgccgc gccgtcgctg atttggccgg cggcaattgc
     4741 actcacttgc tcgtcgggct gcacgagcgc gatccgctgc tgccagcggt cgagcggcgc
     4801 tcggcaactc gcttcacgac gcacctgtat ctcgtctcgt gggacgacga cgatgaaatc
     4861 cgagcgatca tcagccgccg cgtcccgtat ctggaaccag gcacgctcta ggtcacaagg
     4921 aagccacgca tgcgactgtc ggcacgtttg atccagcgca cgagacttac gcagcaagac
     4981 cgggccgcca tgctggccct catgcagacg cattacgaga atgtgcagcc ggcagtattt
     5041 ttccgcgacc tggcggagaa gcggtgggtg atcgtcgttc gcgagccgac cggcgggcgg
     5101 ctgtgcggct tctcgacgca gacgatcctg gaagcggagt gcgacggcca gcttattcgc
     5161 gccctgtttt ccggagacac gatcatcgac cgcgagcact ggggcgatcc ggccctgtcg
     5221 cacgtgtggg gacagtttgc cctggcgctc atcgatcgct acgacgacgc cccgctctac
     5281 tggctgctgc tctcgcaggg ctatcgaacg tatcgcttcc tgccgctgtt ctttcacgag
     5341 ttctatccgc gtcatgacgc ggcgacgccc gagggtctgg agcggtttct cgacacgctg
     5401 gcggaaaggc gctacggggc cgcctacgac cgcaaggccg gcgtcgtccg cgcgcacgcc
     5461 tggcaatatc ggctgcggcg gggcattgcc gacctgtcgc ccgagcggct ccgcgacgag
     5521 catgtgcgct acttcgccga gcgcaatcct ggtcatgacc gcggcgacga gctatgttgc
     5581 ctggctccgc tcacgcgcga gaattttact cgcgcggcgt atcgcgtcat cggacaacct
     5641 gaaccggctc tggagctgac gtaacatgct cgcgcgctgg ctgaatgccg cctggcggtg
     5701 gaagtgcgcc ggcgaagcgg cacgctttca ccgagcgatg actcgcgtcg ccgagacgca
     5761 gcgagatctg ctcctgtcga tgctggcggc caaccgcgag acggcactgg gccgcaagca
     5821 tgtcttcgcg tcaatcaaaa cagtgggcga ctttcagcgg caagttccca tcgctacgta
     5881 cgacgactac gagccctaca ttgctcgcat tgccgccggc gagcccggcg tactcaccag
     5941 cgagcgggtg ctgctcttgg agccgaccag cggcagcacg gccggcgaaa aactgattcc
     6001 ttatacggcc gcgctacgac aacaatttca gcgcgggatc gcggcgtgga ttgccgattt
     6061 gttccggcag cggccacggg cggctcgcgg tagggcttac tggtccattt cgccaacgct
     6121 cggaccgcgc cgcagaaccc ccggcggcat tcccattggc tttgacgagg acgcggccta
     6181 tctgggtctg tttgagcagt gggcgctgcg gcatttgctg gctgtgccca gtgccgtggc
     6241 ccaattgccg gatatcgaca gcgttcgcta tgcgacgctg ctgttcctgc tggcggcgga
     6301 tgacctgagc ctgatttcgg tgtggaatcc cacgtttctg acgtccctcc tggccgccct
     6361 gccgccatgg gctgatcggc tggcggccga tctgcgtctg gggcgattct cgccgccggc
     6421 gccggtggcg ccgcatgtcg cggcgagcct ggcgcgctgg ctgcggccgc accccaggcg
     6481 ggccgacaaa gtcaaatcgc tactgcggga cgcaggggac gacgtccctg gcgaagcctt
     6541 gtggccgcgg ttggcgctca tcagttgctg gaccgatgcc gcggccagtc actttgtgcc
     6601 tgcgctcgcg cgcctctttc cgcaggtcga aatccagccc aaggggctgc tggccaccga
     6661 gggtttcgtg tcactgccgc tggtcggaca ggaaggtgcg gcgctggcgg tgcggtcgca
     6721 cttcttcgag tttgccgaaa cgctcgatcg gtcgcggtgc gctctggctc acgagttaga
     6781 gctcggcggc cgctatcgcg tcatcctcac gaccggcggc ggactttatc ggtacgacct
     6841 gggggacgaa gtggaagtcg tcggcttcgt cggccagtgc ccgactttgc gcttccgcgg
     6901 caagctcacc cgcacgagcg acctcgtcgg cgagaaactc tccgaggccc acgtgcaggc
     6961 gtcgctcgat gacgtcacgc ggcagttgaa tttgcggccc acatttttgc tgctgacgcc
     7021 actcctcgat tcgccgcccc attatcggct ctacgtccag ggagtctctg tcgatgcgct
     7081 gccattactg accagccgat ttcacgagcg gctccaggag aatccgtatt accgccacgc
     7141 gatcgcgttt gagcagcttg ccccgctcga cgccgttttg ctaaagccct cggctgcggc
     7201 ctggtcgctg tacgagcggc agtgcctggc tcgcggccag agagccggaa acatcaagcc
     7261 tgccgtcctc gatcgctgga ccggctggcc ggagattttc actggcgcaa ccgcggaaag
     7321 tcccacgcct gagcacgcgc caatctattc gtgaacactt agcggcagct tctggagcga
     7381 ctcgggcgcg ggctcggtag ccgcgcgcgg cccgagccac tgccgccagt attgccacac
     7441 gtagctgggg cgcggctcat cggcgggcga aacccgcggc agatagaacc aaggcacttc
     7501 cggccgccga tgatggttga ggtcgtattc cccgtgtagc agcacccagg tcatcagccc
     7561 gttgtgcttg aggttccagg ccccctcgat cacatcgcgc ttggaaaacg cgtggccgat
     7621 gtactgccgc gtggaccagt tgaacgaaaa gcaggcatag cagatcagca cggcccgcca
     7681 gtcgagggcc agcagccaga atgccaacgc aaaccagccc acgatgaggg cgacttccaa
     7741 gcggatccag ttgacctcgg cattgcgaat gtcccccagc aaatagctcg aggaccgctc
     7801 ccgctgaaag agccgcgtcc gcagcacgcg cgggcagacg gcaaacagca gagcgcccag
     7861 cggaatcacc ggccagaata ggccgcacag gatgctgtac cactgcaaga cacgcagcag
     7921 ccggttatcg gtcggatagt acatgtcgaa catttcgtga tcggttcggt tccgcaggtg
     7981 atgcccctga tgcgtcacgt ggatcatgct gaaaggcatg ggaaacaaga ggccggttac
     8041 cacgcccagc aaatagttaa cccgctcgtc gctgtggaga ttgcggtgcg acgcctcgtg
     8101 caaaagcgcg tagttggtga gcagcacgta ggagaacagc acgccggcca gaaccgtcaa
     8161 ataccacgcc tgtgcgtgcg acgccagcca cagcagaccg gtggcggcgg caaataccac
     8221 cgccaccagc gcaacattga gtgtgctcgt gatcggcatt tccgccggcc gctggcgacg
     8281 ctccgggctg gctgtcactg ggcaccccgc tttcctgcgg cttgtattta tatcctgttg
     8341 gctttcaggc aaggtgaggg gagacgaaaa gccaggccaa tcgccataat ggctttcggg
     8401 tagattcgtt accggaggtt cgcttgcggg aggagtagcc catgatgacg gacgtcttgg
     8461 ctcgcttagg catcagccgg gagaacagcg gcgtctatgc cggcggctgg atcgaccgcc
     8521 ctgccggcga gatgatcgag tcgatcaacc cggccacggg tgaagtgctg gcccgcgtgg
     8581 cggcggccgg agcggacgat tatgcccggg cggccgaatc atccgccgaa aagtttcgca
     8641 cctggcaatt ggtcccgccc ccccagcgcg gcgagatcgt gcggcagatt ggccaggcct
     8701 tgcgggagta caagagcgac ctggggttgc tggtgaccct cgaagccgga aagattcgct
     8761 ccgaagggct cggcgaagtc caggagatga tcgacatggc cgactttgcc gtcggcctgt
     8821 cgcgtcagct ttacggcctg accattgcga ccgaacgggc caaccatcgc atgctcgagc
     8881 agtggcaccc gctggggccg gtcggcgtga tcaccgcctt caatttcccc gtggcagtct
     8941 gggcctggaa cgcgatgatt gccgccgttt gcggcgatac ctgcctgtgg aagccgtcgg
     9001 agctgacgcc gctgaccgca ctcgcggtgc agaagattgt caatcgcgtg ctcgagccgc
     9061 aggggctggc ggggctgttc aatctggtga tcggcggccg cgacatcggg cagcagctcg
     9121 ccgccgacgc ccgacttccg ctcgtttccg cgaccggaag ctgcgctatg gggaggagcg
     9181 tggccaccac ggtggctggc aggctgggcc ggtcgctcct ggagttgggt ggcaataacg
     9241 gactgatcct cgccgagagc gccaatcagg acttggcgct gcgggccgtg ctgtttgccg
     9301 ccgtcggcac ggcgggccag cggtgcacga cgctgcggcg gctgatcgtg cacgagtcga
     9361 ttgccgagga tttcacgcaa cgtttggcca aggcgtacca gagcattcag ccgggactgc
     9421 cgtgggaaga cggagtgttg ctgggaccgc tgatccggca gcgggccgtg gaacagttcg
     9481 aggcggcgat tgccgcggcg cagaagcaag gcggacaggt cgtctgcggc ggcagtcgca
     9541 tcaaacggcc cggcttttat gtccagccga cgattgttcg agccaaaccc gaaatgccga
     9601 tcgtcaaaca agagacgttt gccccgatcc tgtacgtgat gacatacaaa acgctcgacg
     9661 aggcgattgc cattcacaac ggcgtgccgc aaggcttgtc gagcgcgatt ttcaccgacg
     9721 ggctggcgga tgccgagcgg ttcctctcgc cggtggggag cgactgcggc attgcgaacg
     9781 tgaacatcgg cacgagcggg gcggagatcg gcggcgcgtt tggcggcgag aaggagaccg
     9841 gcggcggccg cgaggcgggc agcgacgcct ggaaggcgta catgcggcgg cagacatgca
     9901 cgatcaatta cggccgcgac ttgccgctgg cccagggggt gaagtttgat atcggaccgt
     9961 agcgctgcga gaatcgccaa tcacaagcaa caagcaatca tctggaattg tacgtgaacg
    10021 ttcgacttgc tctttgggtg gccatcgtgg cggcggtcat tgtcggcacc gggctgctgg
    10081 cgtggacgca gccttggcgc ggcggtgatg cggaccgggc ggcggcccag gccaaggggc
    10141 aaaagctgcc tccctggttt gaagacttca cggacaagtc cggtctgaac tttcgccatt
    10201 ggtgcggcga cggcggcaaa tacttctttc cggaagtcat gggctctggg atcgggctga
    10261 tcgatttcga tcgcgacggg gacctggaca tttttgtcgt ccagggaatg ccggccggag
    10321 gcgccgggga gaaaaagccg ccggctggtt tggagcccgc gccaacgagc cggctctatc
    10381 gccggggaga ggacggccgc catgaggacg tgaccgagga agcgggactc atcgaccacg
    10441 aaccctacgg catgggggtt gccatcggcg acgtgaacaa cgacggctgg ccagatttgt
    10501 acgtctccaa atatggccag gatcggctgt atctcaaccg cgaaggtcgc ttcgaggata
    10561 tcaccgacgc ggccggcatc gacaatcggc gatggggaac cagcgcctgc ttcgtggact
    10621 acgaccgcga cggctggctc gatctgttcg tcgtcaacta cgtcgattac tttccatcgc
    10681 accgctgcgt gcagcccaac ggacagatcg actattgcca tccggggcag tttacggacg
    10741 cgccggcgaa gctctatcgg aatgtaacgg gggacgaaag tccaaaggcc aaagtccaaa
    10801 gtccaaagtc ggagccgggg gggaagggcc aggggtcagc ggagagaggt caggagtcag
    10861 aggtcagagg tcagggggag gagttgcaag tgcgttttcg cgacgtcagt ctcgaggccg
    10921 gcatcgatgc gaaaccgggg ccgggcctgg gcgtgattcc gggcgatttt aacggcgacg
    10981 attggatcga cctgtatgtc gccaacgacg ccaaggctaa tttcctgtgg atcaatcagc
    11041 agggaaagag ctttcgcgat gaagcgattg tggccggtgc ggcggttgac attgccggcc
    11101 ggccgcagtc gagcatggga gtggccagcg gcgacgtgaa tggcgacggc aagagcgacc
    11161 tgttcatgac gcatctcgac ggcgagtaca gcaccctcta tctgcaactc gaagacggcg
    11221 tcttcgaaga ccgcacggtt gcggcggggc tggcagccgc cacgattccc tacacgggct
    11281 tcggcacggc actcgtcgat ctggacctcg acggcgatct ggatttgccg attgccaacg
    11341 ggcgcgtccg ccgacccgat gaaatgcgca cggcggcgga caatccgacg gaattctgga
    11401 aacagtacgc cgagcgcaac cagctctttc tcggcaacgg cgatggcaca tttacggaag
    11461 cggccccagg gggtgacccg ttttacagcc ggccgaacgt ggcacgcggc ctggctatcg
    11521 gggacctcga cggggacggc gatctggaca tggtcactag cgaaatcaac gggccggcgc
    11581 ggatctattt gaatgtagcg cctcggcagg gtaactggct gagcgtgcga gcggtcgatc
    11641 cgcggcacgg cggccgcgac gcatacggcg cactgatcgc cgtcgtcgcc ggcacgcagc
    11701 gctggtcgcg cgacatcaac ccggcataca gctacctctc atccggcgat ccacgcgctc
    11761 acttcgggct gggaaaaatc tcggcgattg atcgaatcga agttcgctgg cctgacggaa
    11821 ccaaggaatc gtttcccggc ggcggcgtga accgcgaagt gacaattcag ccgggcgagg
    11881 ggaagtcgcc atgaaccggc gcgacaataa atcgtcagtg cgccgaatgc cgcggtgggt
    11941 ggtctggctg ttgcttttgc tgggtgcggc ggcgattgcc gccaatgtcg cctggttcag
    12001 gcgcagttcg cgcctgcttc cgatgccggt ggtatcaacc actggcatgg agccggaagt
    12061 agcggagatc atcgaggcgg ggcgacacct ggtcgaagcg gacgagaaat ccgcacagtc
    12121 gtggggagag tatggcgctg tgctgcgggc ccacgaactg ggcccggaag ccaacgtctg
    12181 ttttcgcaat gccgagacgc tcgacccgac ggactaccgc tggccctacc tgctgggcgt
    12241 cagcttgatg acgaccgatg cggacgccgg gctggaggcc tttcgccgtg cggcagccct
    12301 ggcggaggac ctgtctcacg ttcagctccg cctggccgaa acgctcatca acagcggacg
    12361 gtatgcggaa gcggcgccta ttgtcgatcg ggtgctcaaa ggcagaacgc tcgagccgcg
    12421 ggcggaattg gccaaggcgc ggctgctgtt tgaagaggga aaactcgagg agagccgcac
    12481 ctgggcgaaa aaaagcgcgg ccgccgcgcc cgacaagcgg gcgacgcaga tcctgctggc
    12541 gcagatctgt cgccgactgg ggaataagga aggggagcag cgggcacaag cggcagcggc
    12601 ggagcttccc gatattccta ctccctggga cgacccggac gtggaaagcg taaaatccct
    12661 gcgccgcgat cgggcgtggc agcgcctggt tggcgagcga caagccgaca gcggtcagat
    12721 cgccgaggtt gtcgagctgc tggaagaatt gacgctggcc gacctggcag cccgtgcggc
    12781 catcgagccg gtgcgcaggc acctcctgca gggaaatttc gccgaggccg agaaactcct
    12841 tcgcgaaatt gtgcggcagc acgacgagaa cgaaaagctg cattaccagc taggtgtgct
    12901 ctgcttccag caggagcgct acgatgccgc ggtggaggaa ttccgccgcg cggtgtcgca
    12961 caagccggac catgtggacg cgcagtacaa cctgggccac gcgctccgca agatgggtag
    13021 cgacacggag gcgaaagagg cgttcgcagc cgccgttcgt ctcagcccca gccacgcacg
    13081 agcgcgggcc aatctggccg agctgctgtt tgacgccggt gagcccgagg aggcgcggca
    13141 gcatctggcg gtggctgccg agctggcccc gcaagatccc aaagttcgcg agctgctggc
    13201 gcgcgatcgc agcgcgaagg actagctgcc gtggtcgcct tgcccagcgg ctggcttcgt
    13261 ctatgatccg tccatgaaca tcgcttgggt cctgctcgcc tattgcattc tactcatcgc
    13321 cgcctcgctg gccggcggct ggctgccgct gctcaccaaa ctcacgcacc gctggatgca
    13381 attggccatg agcaccgtcg gcgggttcat gctgggcgtg gccctgctcc atctcgtgcc
    13441 gcactcgttt gccgtgctgg gaaatctgga tttgacagtc ggctggacgc tggccggcct
    13501 gctggcgatg ttcctgctga tccgcgtgtt ccatgtccat gcccacgagc acggcgacac
    13561 ggggcacgaa catgaccacg agcatgaggc caagtgcgat catccggatc acgagcacca
    13621 tccgcatccc gatcccgagc atgcctgtac gcacgacggc actcgcgaac gggccggcag
    13681 cggccgacac caattcagtt ggatcggtct ggcgctgggc ctgacactgc attccgtcat
    13741 cgacggtttg gcgctaggcg ccgcggtcat ggcagatgcc gacgaaggca atgcgccaaa
    13801 agggctggca ttgttcggct tgggaacgtt tctggccgtg gtgctgcaca agccgctcga
    13861 ttcgctgtcg atcacctctc tgatgatggc cggcggctgg tcgcggcgtt cggcagtgct
    13921 ggccaatgtg gtatttgcgg tgatctgccc cctcggcgcc gtgggctttg tgctcgggct
    13981 gaaccagctt tcctcgcagc aacacgtcgt cgtcggctgt gcgctggcgt ttgccgcggg
    14041 cgtgttcctg tgtatttcgc tggcggatct gcttccggag ctgacgtttc acgcccatga
    14101 tcgcttgccg ctgactgccg cgctgttcgt gggcgtggcc ctggcctggg cgattggtta
    14161 cttcgagccg ccgcacgcgc atggtccgca cgagcgcggg ccgcacgatc atcgcgaggc
    14221 tgagcacggc gaccacgggc actagacgtt tcggtactgc catgctctgc atccgcccct
    14281 ttgccgacgc cgacgttcaa ttcggcatgc gactgaagga gcaggccggc tggaaccagc
    14341 ttgagggaga ctggcggcgg atgctggaca tgcagcccga tggctgcttc gtagcccagt
    14401 tcgacggcgc cgcggctgga acgtgcatga cctgcatctt cggcgacgtc gcctggatcg
    14461 ccatgatgct ggtcgagcag tcgctccgcg gcaaaggcat cggccgggcg ctgatggaac
    14521 atgcgctcgg atttctcgac ggccgcggca ctggcacgat ccgcctggac gccacgccga
    14581 tgggccagcc tctctacgag aagctcggat ttcaggcgga gtattcgctg gccaggtatg
    14641 cggggaagcc gactggatgc cagcgacctt caggagcgca tcggcacacc cgcgactgcg
    14701 acgaattggt ctattcgctc gaccgctcgg tcaccgggtg cgaccgccgc aagttgctaa
    14761 aacgcctgat cgacgagcgc gactgctggg tcgttggcgg cagccccctc cgcggttacc
    14821 tgctgacacg tgccggctca agagcgaccc aaattggccc gtgcatcgcc gacgacattg
    14881 ctggcccggt cttgcttaat cacgccttcg accaacattc tgaaagcccg atctacatcg
    14941 acatcccgct ttcgcaccag ccggccgccg cgcttgcccg cgaggaagga ttgaccgcgc
    15001 agcgccaatt gctgaggatg tgccgcggaa ggcgcgtcga cgagcaagtc gaacgtttgt
    15061 gggccagctc ggggccggag ctggggtaag taggccgcga ctccgtcgcg tgccgggaag
    15121 ttcttaaggc ctggtctgct gtagaagctg gcgcgccgcg gagcggcagc ctacttgtac
    15181 agccccggct catcggtccc atcgccgttg aagtcgccca cgaccggcgt ctgcgtcgca
    15241 tcccccaggt cgagctgctg gtcctcgggg tcgatctggt ggttgccgtt ggtgtcgatg
    15301 atccagcggc cggcgcggta gatgccgatc tcgtcgatcc cgtcgccgtt gaagtcgccg
    15361 acaatcggct ggtccccttc ctcgccgaac tgggcaattt cgtccccgtg ggagaatcgt
    15421 ccgtcgccgt cggtgtcgaa ccgccactgc ccgtttttga agatgccgat ggagccgatg
    15481 ccgtcgccgt tccagtcgcc agatactggc acgtcgccgc cgctgccaaa ctcgaacacg
    15541 tggtcgatca ggtcggagcg ctcctgcccg ctggaggtga gccgcatcag gcgctctccc
    15601 ttggtcgcgt gccgctcgtc gggcggcaca ttcttgggcc gcatgcggac ggcattttgc
    15661 ggatcgggca gacctggctc gttggccagg tgttggcggt cgccgggcca ctcggggccg
    15721 tagatgccaa tatcgtcctt gccgtcgccg tcccagtcgc cgaccaccgg tcggtcgctt
    15781 tggctgccga gctgggccca caggtcgtcg ccatcccagc gctgattgcc gttgaggtcc
    15841 agcagccatt cgccgcggaa gt
//
LOCUS       DASRJB010000096         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1721252, whole genome shotgun sequence.
ACCESSION   DASRJB010000096 DASRJB010000000
VERSION     DASRJB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721252"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VFB80_02910"
     CDS             complement(<1..1371)
                     /locus_tag="VFB80_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32733.1"
                     /translation="MKASVPLLTGCVALGLTLPATLCQAAHPAAGALTITAASFGQLG
                     FGGARSGSASGDVRRKSEDLLNQARKAIKQGDFVAAESFIAEAEKLDAKYDVLTERFV
                     DTPAKIRKLLAEERVKAGASGRLPAQPAAAPIPGDPYALRQVANPQAPNLLSPESAAE
                     RLLDDGKSKAQSHLKDARAALAAGDKFAALAAWQRAAAIPATYAPGEDSPQTVAQELA
                     RAGVDTSRLKPPMPPAPYALRPSDVESQSDRVPPFSANPNAIPAAAVSGNLSPYNLPP
                     ETNPLAASPASPAGGGQFQPPDQSAPQAPGQFTPIGGYPEPQRLNPGAAQRLPVGDSG
                     AKSQAASLVAQARLALDKGDLRAAQQFADQAEALNIPDTAFEAGETRPWQISLEISRA
                     MYRREGVQPASATGPAAVEPKYPVAQGMYNPATDTTKIIPANSQGSVLARTQATGEAS
                     SGARLYQ"
     gene            complement(1819..3243)
                     /locus_tag="VFB80_02915"
     CDS             complement(1819..3243)
                     /locus_tag="VFB80_02915"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZN32734.1"
                     /translation="MTTYRIEKDSMGEVQVPAQAYYSAQTQRAVENFPISGWRLPHPL
                     IRALGLVKLACATANRDLGKLTQTGKYRLSDQQVEQLLAACREVADGRFDGEFPIDVF
                     QTGSGTSSNMNTNEVIANRAIELLGGDRFQKEKPVHPNDHVNMGQSTNDIFPTAIHVA
                     VAVEIQKQLIPALQRLHEVLAKKAAEWDQVIKIGRTHLADATPLRLGQEIGGFSRQIE
                     LSIERARRAIVPVLELPAGGTAVGTGINTHPEFGARVAAVLADETGLAFVEAKDHFEA
                     NAQRDGLVESHAQLRTIAMTLFNVSNNIRWLGSGPRCGFYEVKLPDRQPGSSIMPGKV
                     NPVMCESMMQVCARVLGNDQTIVFSGANGGQFQLNIMMPVMGHAALESVHLLAAATRA
                     FVNFCVLEMEANPEACEASVEKSLSMVTALNPHIGYERAAAIAKEAFKTGKTIREICR
                     EQGILPESTLREALDPMSMTQPHE"
     gene            3391..4548
                     /locus_tag="VFB80_02920"
     CDS             3391..4548
                     /locus_tag="VFB80_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN32735.1"
                     /translation="MAAPPDIQFRDSPVVDAELVAPSGKPCAACATPVEPLDKFCPAC
                     GTANPDYRPPNSSPGHPPGGSAPREERISRSEMTTVSKHFKCQQCGAEVATDPNQRSY
                     VCPFCDSTYVLEFSPQETGRQQPEFVIGFAITPEQAQEAFRKWLGDNSWYRPGDLATA
                     SIADKLKGIYLPFWAFTMLAQSQWRAQIGEHWYRTETYTETDSKGNTVTKTRQVQETE
                     WWPLAGRHHRYYSGYLVSGSKGLAHDQSLRIQPFQLPALKRYEPSFLAGWLAEEYSVE
                     QGAALAICQQEFQRQEQRNIGSFLPGDTHSGLAVETRFSSVTSDLCLLPIYILSYRYQ
                     DKLYRFLLNGQTGKLAGDKPVSGTRIGIAVAVGLAIVLLVVLIGVILANWR"
     gene            4724..>5607
                     /locus_tag="VFB80_02925"
     CDS             4724..>5607
                     /locus_tag="VFB80_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32736.1"
                     /translation="MSDVLTKCSICQALLDEEDLFCANCGAEAPQGEERRQAGRTLLA
                     THNFECQGCGASMSYDASAQKLRCPFCGSEKLEEQRDVKTLRPQWVVPFAISQADALA
                     RLRQWLGSSFWRPGDLSTRAEVTTLTQVYVPYWVFAARVFTFWTADTSQTPGGARGDW
                     FPLAGENRASYSGLIVGASSVLTPAETSAISPYDLSQAVPPEQVDLDNVVYEQFRVQR
                     KYARPLAQQGLENLESQACRQFVKGNCRNLKVNVRVEGLTGEPVLLPAWIMAYRYRDQ
                     VYRFLVNGQSGRCTGTAPT"
BASE COUNT          912 a         1817 c         1901 g          977 t
ORIGIN      
        1 ctgatacagc cgcgcgcccg acgaggcctc gcccgtcgct tgcgtacgcg cgagcaccga
       61 gccctgcgaa ttggcgggga tgatcttggt cgtgtcggtc gccgggttgt acatgccctg
      121 cgcaaccggg tacttcggct ccacggccgc cggaccggtg gcgctggccg gctgcacgcc
      181 ctcgcggcga tacatggccc ggctgatttc aagcgagatc tgccaggggc gcgtttcgcc
      241 cgcctcgaag gccgtatcgg gaatgttcag tgcctccgcc tggtcggcga attgctgcgc
      301 cgcgcggaga tcgcccttgt cgagcgccag gcgcgcctgg gccaccaggc tggccgcctg
      361 agacttcgcg cccgaatcgc ccacgggcaa gcgctgcgct gcaccggggt tcaatcgctg
      421 cggctcggga tagccgccga tcggcgtaaa ctgccccgga gcttgcggtg cggactgatc
      481 gggcggctgg aactggccgc cgccggccgg actggcaggg ctggccgcca gcggattcgt
      541 ttccggcggc agattgtacg gagaaaggtt gcccgagaca gccgccgcgg ggatcgcatt
      601 gggattggca ctaaacggcg gaacgcggtc ggactgcgac tcgacgtcgc taggacgcag
      661 cgcgtagggc gcgggcggca tcggcggctt gaggcggctg gtatccacgc ccgcgcgggc
      721 cagctcctgg gccacggtct gcggcgagtc ttcaccgggg gcgtaggtcg caggaatcgc
      781 ggccgctcgc tgccatgcgg ccagcgcggc gaacttgtct ccggcagcca gggccgcgcg
      841 ggcgtccttg aggtgcgact gggccttgga tttgccgtca tcgagcagcc gctcggccgc
      901 gctctcgggc gacagcaggt tgggcgcttg cggattggcg acttgccgca gcgcgtaggg
      961 atcgccgggg atcggcgcag cggcgggctg cgccggcagg cggccgctcg cgccggcctt
     1021 cacgcgctcc tcggccagaa gcttgcgaat cttggccggc gtatcgacga atcgctcggt
     1081 gagcacgtcg tacttcgcgt cgagcttttc ggcctcggca atgaacgact cggcggccac
     1141 gaagtcgccc tgcttgatcg ccttgcgggc ctggttgagc aggtcttccg attttcgccg
     1201 gacgtcgccg gaagcactgc ccgagcgagc gccgccaaaa ccgagctggc cgaaactggc
     1261 ggcggtgatc gtcagggcgc cggccgccgg gtgggcagcc tggcacaggg ttgcaggcag
     1321 cgtcagacca agggcgacgc atccggtcag cagtggcacg ctcgctttca aagcgattct
     1381 cctggtacgc atggcatctt gcgccgtatg gcgctcccgc ggatttttgc gctgcgaagg
     1441 cggccgctcg aaccggcgca tggtcgccgc agcgctcccg cagcgccgac acctggcgac
     1501 cagcacgatt tggcccattc agccgctgcc acgtcccaag cggaacatcc ggcaagcgga
     1561 caatcccggc caagacttgt ggcggtagat gagaggcggg taattaccgg ccgacccaga
     1621 gcgggtcaag agcagtttga gcgagctcgg cgaaattttt tcggaattcg gcgcagaacg
     1681 cgagcggcag ccgcgaagcg actcgcggtg aaaatgccga acccgcttga atcggcaatt
     1741 agcgcggcgg accttagcga aactcgccga acccgacaag atcgctctgt ttccctccca
     1801 gaggagaggg agagcgtgct actcgtgcgg ctgcgtcatg ctcatcgggt cgagggcttc
     1861 gcggagcgtc gattcgggca ggatgccctg ctcgcggcag atctcacgga tcgtcttgcc
     1921 ggttttgaag gcttccttgg cgatggcggc ggcccgctcg tagccgatgt gcgggttgag
     1981 ggcggtgacc atcgagaggc tcttctcgac cgacgcttcg caggcctcgg ggttggcctc
     2041 catctccagg acgcaaaaat tgacgaaggc ccgcgtcgcg gcggcgagca ggtgcacgct
     2101 ttccagggcg gcgtggccca tcacgggcat catgatattg agctggaact gcccgccatt
     2161 ggccccgctg aacacgatcg tctgatcgtt gcccagcacc cgcgcgcaga cctgcatcat
     2221 gctttcgcac atgacgggat tgaccttgcc gggcatgatc gagctgcccg gctgccggtc
     2281 ggggagcttc acttcgtaaa agccgcagcg cgggccgctg ccgagccagc gaatgttgtt
     2341 gctgacattg aacagcgtca tggcgatcgt ccgcagctgg gcgtgcgatt cgaccaggcc
     2401 gtcccgctgg gcgttggctt cgaagtggtc cttggcctcg acaaaggcca gccccgtttc
     2461 gtcggccaaa accgcggcca cgcgtgcgcc gaactccggg tgcgtgttga tgccggtgcc
     2521 gaccgccgtg ccgccggcgg gcagttccag gaccggcacg atggcccgcc gggcccgttc
     2581 gatcgacagc tcgatctgcc gcgaaaaccc gccgatctcc tggcccagcc gcagcggcgt
     2641 ggcatcggcc agatgcgtgc ggccgatctt gatgacttgg tcccactcgg ctgctttctt
     2701 ggcgagcact tcgtgcagcc gctgcagggc gggaatcagc tgtttttgaa tctcgaccgc
     2761 gaccgcgacg tgaatcgccg tggggaagat gtcgttggtg ctctgcccca tgttgacgtg
     2821 atcgttggga tggaccggct tctctttctg gaagcggtcg ccgcccaaga gctcgatcgc
     2881 ccggttggcg atcacctcgt tggtgttcat gttgctcgac gtgccgctgc cggtctggaa
     2941 cacgtcgatc gggaattcgc cgtcaaagcg gccgtcggcg acttcgcggc aggctgccag
     3001 cagctgctcc acctgctggt cgctgaggcg gtacttgccg gtctgggtca gcttgcccag
     3061 gtcgcggttg gccgtcgcgc aggccagctt gaccaggccc agggcccgga tgagcgggtg
     3121 cggcaggcgc cagccggaga tcgggaagtt ctcgacggcc cgctgcgttt gcgcgctgta
     3181 gtaggcctgg gcgggaacct ggacttcgcc catcgagtcc ttttcaatgc ggtaggtcgt
     3241 cataggtcaa gatcgcgaga agaattgcga atactggcaa actgagaatc gtagccgcga
     3301 ctcccctggt gggtcaaagg cttgctgcgc ggctcggcgg cgttacaact actcgataca
     3361 tcattctatc gcgacgcgca gaaccacgcc ttggccgctc ctcccgacat tcagtttcgc
     3421 gattcgcccg tggtggatgc cgagttagtc gcgccctcgg gcaaaccctg cgcggcttgt
     3481 gcgaccccgg tcgagccgct cgacaagttc tgcccggctt gcggaacggc caatccggat
     3541 taccggccgc caaatagtag tccggggcac ccgcccggcg ggtcggctcc gcgtgaggaa
     3601 cgcatctcgc ggagcgagat gactactgtg agcaagcact tcaagtgcca gcagtgcggc
     3661 gcggaagtcg cgacggaccc gaaccagcgg tcgtatgtct gcccgttctg cgactcgacc
     3721 tatgtgctgg agttctcgcc gcaggaaacc ggacggcagc agccggagtt cgtcatcggc
     3781 ttcgccatca ctcccgagca ggcgcaggag gcgtttcgga agtggctggg cgacaacagc
     3841 tggtatcggc ccggcgacct ggcgacggcg tcgatcgccg acaagctgaa gggcatctat
     3901 ctgcccttct gggcgttcac gatgctggcc cagagccagt ggcgggcgca gatcggcgag
     3961 cattggtatc gcaccgagac ctacaccgag acggattcga aaggcaacac ggtcaccaag
     4021 acgcggcagg tgcaagagac tgaatggtgg ccgctggccg gccgccacca ccgctactac
     4081 agcggctacc tggtcagcgg cagcaaaggc ctcgcccacg accagtcgct gcggattcag
     4141 ccgttccagc tgccggcgct caagcgctac gagccgtcgt ttctggccgg ctggctggcc
     4201 gaggaatact cggtcgagca gggcgcggcg ctggccatct gccagcagga atttcagcgg
     4261 caggagcagc gcaatatcgg ctcgttcctg cccggcgaca cgcacagcgg cctggcagtg
     4321 gaaacgcgct tcagcagcgt gacctccgac ctctgcctgc tgccgatcta catcctcagc
     4381 tatcgctacc aggacaagct ttaccggttt ctgcttaacg gccagaccgg caagctggcc
     4441 ggcgacaagc cggtgagcgg cacgcggatc ggcatcgcgg tcgcggtcgg tttggcgatt
     4501 gtgctgctcg tcgtcctgat tggggtgatc ttggcgaatt ggagataggg ggtcgggggt
     4561 cgggcgtcgg gagtcgggaa agaagtgcgg cctcgattcc tgctacgtat tccgtactca
     4621 gtaccgagta cgaagtaaca aaccagcgtt tctcctattc ctgacctctg aactctgagc
     4681 cctcctcgac tcccgacccc cgatcctcga cccccgactc ccgatgtccg acgtcctgac
     4741 caaatgctcg atctgccagg ccttgctcga cgaggaggac ctgttctgcg cgaactgcgg
     4801 ggcggaagcg ccgcaaggcg aggagcgccg gcaagccggg cgcacgctgc tggccacgca
     4861 caacttcgag tgccagggct gcggggcgag catgagctac gatgcctcgg cccagaagct
     4921 ccgctgcccc ttttgcgggt cggagaagct cgaggagcag cgagacgtca agacgctgcg
     4981 gccgcaatgg gtcgtgccgt tcgctatttc gcaggccgat gcactggcgc ggctgcggca
     5041 gtggctgggg agcagcttct ggcgaccggg cgatctctcg acgcgggcgg aggtgaccac
     5101 gctgacgcag gtctatgtgc cctactgggt ctttgcggct cgcgtcttca cgttctggac
     5161 cgccgacacc agccagacgc ccggcggcgc gcgcggcgac tggttcccac tggcgggcga
     5221 gaaccgcgcg agctacagcg gcctgatcgt cggggccagc agcgtgctca ctcccgccga
     5281 gaccagcgcg atctcgccct acgacctgtc gcaggcggta ccgccggagc aggtcgatct
     5341 ggacaacgtg gtctatgagc agttccgcgt ccagcgcaaa tatgccaggc cgctggccca
     5401 gcagggactg gaaaacctgg aaagccaggc ctgccggcag ttcgttaagg gcaactgccg
     5461 caacctcaaa gtcaacgttc gcgtcgaagg actcaccggc gagccggtcc tgttgcccgc
     5521 ctggatcatg gcctaccgct accgcgatca ggtctatcgc ttcctcgtca acggccaatc
     5581 cggccgctgc accggcaccg cgccgac
//
LOCUS       DASRJB010000097         9373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1439037, whole genome shotgun sequence.
ACCESSION   DASRJB010000097 DASRJB010000000
VERSION     DASRJB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439037"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFB80_02930"
     CDS             complement(<1..1096)
                     /locus_tag="VFB80_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32737.1"
                     /translation="MANRDSLGGIIHTYQKYDPVNLPAPTQPPPDVVSPALEHFLMFG
                     DARGLTDEELARAVRLDPSQIAGLGPSLDALVAMLLERKRKILAKYETESVQGLARKE
                     FQRSGDKIDPPPNLKKRFQQAFQDEQLRDLEMLWYSAEGERSRFARQIVALVEHLGNK
                     YQVDELASKYAFSGRTPLTIPEALAVKEELEKIDELLKQLEEARKHATIGIIDLELLE
                     HFTEPGDLARLEELQRLIENYVREMAERQGLERDGKGFRLTPKAYRLFQGRLLERIFA
                     QLQESRSGRHQGPIVGEGAVEMSQTKPYEFGDSLAHMDIPQSFVNAMLRTGGGLPVKL
                     GQEDIVIHRTRNSPKCATAVIMDMSGSMRYD"
     gene            complement(1113..1487)
                     /locus_tag="VFB80_02935"
     CDS             complement(1113..1487)
                     /locus_tag="VFB80_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32738.1"
                     /translation="MGSTGRGYPLGALFVLVTASAVLVAGIAPLVRLVAKGDAVNTGQ
                     LVMGVLAGINSGCLIGAALGLLQFRKLLGTALGATVGTLIGAAAGGLAMLPASQLITA
                     AIPMTAGCGLIVGVALLMRRTA"
     gene            complement(1490..2443)
                     /locus_tag="VFB80_02940"
     CDS             complement(1490..2443)
                     /locus_tag="VFB80_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32739.1"
                     /translation="MKRTAFTLVELLVVIAIIGVLVALLLPAVQAVREAARRSSCSNR
                     LSQLIIAVHNYEMAHGCYPAGTLDAAGPVANAPVGYHHNWLIQILPYLEEQNAWKAID
                     KKVGVYAPQNAAVAASPPPNLTCPSGTVPRNANLSCYAGVHHDLEQPIDATNNGVFIL
                     NKCIRYDDVTDGASHTLYIGEKVPDAWDLHWMSGTRATLRNTGMPLNWWTYSSGLPEP
                     GESTEGIQLPMGYDEAVGAPAAAPGSAPADPAAAAAPAAPKPAAPGSPLYVGGFGSFH
                     PNGTQFARGDGGVQWLSNSIAPTVYQQLGHRRDGKLLPRDW"
     gene            complement(2466..3455)
                     /locus_tag="VFB80_02945"
     CDS             complement(2466..3455)
                     /locus_tag="VFB80_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32740.1"
                     /translation="MTRRLRAFTLIEMLAVIGIIGALVALLLPAVQSAREGARRFQCQ
                     NHLSQLILAVHNYESLHGIYPPGTIDAKGPILNAQVGYHHNWIVQILPYCESTPLWNS
                     IDKRVGVYHARQLAALGSPPKFLDCPSCSAPQGNGCYVGVHHDTEKPIDAKDSGMFFL
                     NSRLRIEDVSDGLTHTLFLGEKLPDGWDLHWMSGTRATLRNTGSGINELNYRNGLPRA
                     RSIDDLTEPGVDWGDPAAMPGLGEIPGVETSPTPQNPDEPEFPTLPLAPALAAGGPGN
                     PLWVGGFASDHAVGANFAFGDGSVRLLSNETDLTVLQRLANRKDGQLARLQER"
     gene            complement(3452..3838)
                     /locus_tag="VFB80_02950"
     CDS             complement(3452..3838)
                     /locus_tag="VFB80_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32741.1"
                     /translation="MKIKRRAAALVIALVTLMVVMLIAGTVARSLVGSHRQSRRAGDE
                     VQAQWLAEAALARGLVKLRSQADYSGETWRPEVTPANVGVAEIRVERPADQPARIVVQ
                     ARYPDDPWQRASVHREVPLAPKEDAP"
     gene            complement(3835..4332)
                     /locus_tag="VFB80_02955"
     CDS             complement(3835..4332)
                     /locus_tag="VFB80_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN32742.1"
                     /translation="MTNLRRAKTLVEVMVIITILSVVLAASTTTLAALLKLERQFRRD
                     AEQSTMLARLGAQFRGDVHQAKDAAVGEDCVLTWPDGRAVRYAAGDREITREVRRGDA
                     VEHRDSFRLPAPAAVNLAKVEQPGGSLVRLTIRPDGSPATGSPPIATTTIDAALALQQ
                     KEALP"
     gene            complement(4329..4736)
                     /locus_tag="VFB80_02960"
     CDS             complement(4329..4736)
                     /locus_tag="VFB80_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN32743.1"
                     /translation="MNSRSRQRGMTVVELTVSLVVLGVAMAALVQLVALAASQRRAMA
                     QRRIALQEIANQAERIALLPWSETAPDKLTGWQPSAELSEVLPAAQCSIAVSEEPAAP
                     ARKIRLEVAWTNSVGQPMEPVGLSIWRFGEAAP"
     gene            complement(4764..6041)
                     /locus_tag="VFB80_02965"
     CDS             complement(4764..6041)
                     /locus_tag="VFB80_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN32744.1"
                     /translation="MNEPLGPLTLVLLAIPALALRLAVRALYGQRRLAVADPMQSLLS
                     MASTIMFLMAGLGLLVGIMGFWFLIIPLPFIAVVLLLMIIDRLRHSEHRALVWALAAA
                     AQKGIPLSETARAYADETLGDTGVRSLALAEAIERGEPLAQAVHTARLRLGTAMKLAV
                     RLGERLGMLGPAMRQQLENSHEVDMALQTALARFFYLGTVTWVLSGVTTFTMLKIVPV
                     FQKMFEEFGLKLPAMTRLVVNASSWFVEVGWVFLLPFLTLAMPLFFLIAILYYIGWLP
                     RNIPGWWLLFRRFDGALVMRGLGLAVRQGRPLPEALRMVADCYPLSIVSGRLRRAAER
                     VEAGQDWCDALRQTGLIGWTDAALLSAAERTGNLEWGLAEMADSAMRRQAHKIQALVN
                     VLFPVILVVLGMFAALFTIGMFLPLISLIQGLA"
     gene            complement(6054..7118)
                     /locus_tag="VFB80_02970"
     CDS             complement(6054..7118)
                     /locus_tag="VFB80_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN32745.1"
                     /translation="MITIVDEQYSSDVPVLSAAAAEAVLDRAAQITAAGMPLAAGLRA
                     SAAECDSWRLSRGLKSIANEVERGRSLEDCLAISIRRLPPHLVGLIQAAQRSGAIAPM
                     LVEWLQNRRAARAHWRAIVAALAYPVFSLLMAIGVYILFAVEVVPPFKQMFEEFGLRL
                     PAMTVQFLRSCEVAPPLLAFLGVGIVATAVTTRVFGGRAGWSWLISSLPLIGNAWHWT
                     GVAEMLRCLGLLVEQRVPLPEALRLTAGGISDAYVGGQCQALARQVEQGTSLTMSLVK
                     LRTLPLSIVPLVHWGENHDALGLGLRSAAEMLEGRLNLRANLLGQVLPPILFITVTMA
                     IASGAISLFLPLISLIQGLS"
     gene            complement(7115..8185)
                     /locus_tag="VFB80_02975"
     CDS             complement(7115..8185)
                     /locus_tag="VFB80_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN32746.1"
                     /translation="MTISATLARPPLTLEQLAALSDEIGALVRAGVPLDQGLREMARE
                     LPGRLGRFAGEMGKRIDAGQPLPQVVAELGSSLPPAYQTLIAAGLRAGRLPAALEGVA
                     RTARRISQLRRAIGLALIYPVVLLLLTWGLGLFVLTKLAPVFARMLDEFDVTRLPVGQ
                     WVDRAAESSWVWGPLVPALITVWLVVAWYRSGRVAYGYELHPLLAFGAVGTLARMQRA
                     GRMASLAELLGLLLHHSVPLAEAVELATGACGSGNLARGGKQLAQQLRRGERIDEPPA
                     GFSPLLAWTVASGQSQPQLCRTLARTAEVYREEFDRRGQWLTVYVPLALTVGLCGTVA
                     SVYAFVALGPWIAIMMRLAQPY"
     gene            complement(8257..>9373)
                     /locus_tag="VFB80_02980"
     CDS             complement(8257..>9373)
                     /locus_tag="VFB80_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HZN32747.1"
                     /translation="AQQRVQVRPHLSKHAAGFPPVENLLACIQSGNRERGIAQIGAGR
                     DTAGSAWIIRAADRADSRPLNVTIWGGQTDFAQALWRVREDRGPDGVRSFIKKLRVYD
                     INDQDKIQSWLFENFPDLFYVLASAEPGRDKREGVYRGMYLGGDESLTSLAWLDEHVR
                     QNHGPLGALYPAKTWTAPNPHSALKEGDTPSWFYFRPSGLQDPSEPGWGGFGGRFKQA
                     ERGLWRDAADFVDGKSDVRAAVWRWRDIWQRDFQARMDWCVQETKNANHAPAVVIDGA
                     AAARPLDRKAVAGQPLTVSASASDPDGHRVQYKWWIYADAGTYRGLVKVRSELTAAAT
                     VEIPPDAAGKTLHLILEATDNGTPPLTTPVRIVIRVD"
BASE COUNT         1611 a         3183 c         3092 g         1487 t
ORIGIN      
        1 cgtcgtagcg catcgagccg ctcatgtcca tgatcacggc ggtcgcgcac ttgggcgagt
       61 tccgcgtgcg gtggatgacg atgtcttcct gccccagctt caccggcagc ccgccgcccg
      121 ttcgcagcat cgcgttcacg aacgactgcg ggatgtccat gtgggccagc gagtccccga
      181 actcgtaggg cttcgtctgc gacatctcga ccgctccctc gccgacgatc ggtccttgat
      241 gccggccgga ccgcgactcc tgcagctggg cgaaaattcg ctcgagcagg cggccctgaa
      301 agaggcggta cgctttgggt gtcaggcgaa agcccttgcc atcccgctcc agcccctgcc
      361 gctccgccat ttcgcggacg tagttctcga tcagccgctg caactcctcg aggcgggcca
      421 ggtcgcccgg ctcggtgaag tgctccagca gctccagatc gatgatcccg atcgtggcgt
      481 gcttgcgagc ctcttccagc tgcttcaaaa gctcgtcgat cttttccaac tcttccttga
      541 cggccagggc ttccggaatc gtgagcggcg tacggccgct gaacgcgtat ttggacgcca
      601 gctcatcgac ctggtacttg ttccccaggt gctcgaccag cgcgacgatc tgccgcgcga
      661 aacgcgatcg ctccccctcg gccgagtacc agagcatctc caggtcgcgg agctgctcgt
      721 cctggaaggc ctgctgaaac cgcttcttca ggttcggcgg cggatcgatc ttgtccccgg
      781 agcgctggaa ctccttgcgg gccagcccct gcaccgattc cgtttcgtat ttggccagga
      841 tcttgcgctt ccgctccagc agcatcgcca cgagggcgtc caggctcggc cccaagccgg
      901 cgatctggct cggatccagc cgcacggccc gcgccagctc ttcgtcggtc agcccgcggg
      961 cgtcgccgaa catcaggaag tgctcgagcg ccggcgaaac cacgtcgggc ggcggctgcg
     1021 tcggcgcggg caggtttacc gggtcgtact tctggtacgt gtgaatgatc ccgccgaggc
     1081 tgtcgcgatt ggccatgctg gcattttacc gattacgcgg tgcgtcgcat cagcagggcg
     1141 acgcccacga tcaggccgca gcccgcggtc atcggaatgg cggcggtgat cagctggctg
     1201 gccggcagca ttgcgagtcc gccggctgcc gcgccgatga gcgtgccgac cgtagccccc
     1261 aaggccgtgc cgagtagctt gcgaaactga agcaggccca gcgccgcgcc gatcaggcag
     1321 cccgaattaa tgccggccag cacgcccatg acgagctgcc cggtgttgac ggcatctcct
     1381 ttggcgacca gccgcaccag cggcgcaatg cccgccacga gcacggccga ggccgtgacc
     1441 agcacaaaaa gtgcaccgag cggatagccg cggccagtcg atcccatcgt caccagtccc
     1501 gggggaggag tttgccgtcg cgacgatggc ccagttgctg atacacggtc ggcgcgatgc
     1561 tgttcgataa ccactgcacc ccgccgtcgc cgcgggcaaa ctgtgtccca ttcggatgga
     1621 agctgccaaa tccgccgacg tagagcgggc tgcccggggc ggccggcttt ggcgccgcgg
     1681 gagcggcagc agccgcaggg tcggctggtg cgctgcccgg cgcggctgcc ggcgcgccaa
     1741 ctgcctcgtc gtagcccatc ggcagctgga ttccctccgt cgattcgccc ggctcgggca
     1801 ggccgctgct gtaggtccac cagttgagcg gcattccggt atttcggagc gtggcccgcg
     1861 tgccgctcat ccaatgcagg tcccaggcat cgggcacctt ctcgccgata tacagcgtgt
     1921 gcgaagcgcc gtcggtcacg tcgtcgtagc gaatgcactt gttgagaatg aaaacaccat
     1981 tgttcgtggc gtcgatcggc tgctccagat cgtgatgcac gccggcgtag cagctgaggt
     2041 tggcatttcg cggaacggtc ccggacgggc aagtcagatt cggcggcggc gacgccgcaa
     2101 cggccgcgtt ctgcggggcg tagacgccga ccttcttgtc gatggccttc caggcgttct
     2161 gttcctcgag gtagggcaga atctggatca gccaattgtg gtgatagccg accggggcgt
     2221 tggcgaccgg gccggcggcg tcgagcgtgc cggccgggta gcagccatgg gccatctcgt
     2281 agttgtgcac cgcgatgatg agctggctga ggcgattgct gcaactggag cggcgagccg
     2341 cctcgcggac ggcctgcacg gccgggagga gcagcgcgac gagcacgccg atgatggcaa
     2401 tcacgacgag caactcgacg agcgtaaagg cggtgcgttt catcggaggg gctccggaat
     2461 gttgtttatc gttcttgcag ccgcgccagc tggccgtcct tgcggttggc caatcgctgg
     2521 aggaccgtca ggtcggtctc gttggaaagg agtcgcacgc tgccgtcgcc gaaggcaaag
     2581 ttggccccga ccgcatggtc gctggcaaag ccgccgaccc acagcggatt gcccggtcca
     2641 ccggcggcga gggccggagc cagtggcaac gtcggaaatt cgggctcgtc aggattctgc
     2701 ggtgtcggcg atgtctcgac gccgggtatt tcccccaggc cgggcatcgc ggcgggatcg
     2761 ccccagtcaa cgcccggttc ggtgaggtcg tcgatgctgc gggcccgcgg caagccgttt
     2821 ctgtagttga gctcgttgat gccggagccg gtgttgcgga gtgtggcccg cgtaccgctc
     2881 atccaatgca ggtcccagcc gtcgggcagc ttctcgccca ggaaaagtgt gtgcgtgagc
     2941 ccgtcggaga cgtcctcgat ccgcagccgg ctgttgagaa agaacatgcc gctgtctttg
     3001 gcatcgatcg gcttctcggt atcgtggtgc acgccgacgt agcacccgtt cccctgcggg
     3061 gccgagcacg acgggcagtc caggaatttt ggaggcgatc ccaaggcggc cagctgccgg
     3121 gcgtggtaca cgcccacccg cttgtcgatc gaattccaga gcggcgtgga ttcgcaatac
     3181 gggagaatct ggacgatcca gttgtggtga tagccgacct gggcgttgag gatcggcccc
     3241 ttggcgtcga ttgtgccggg cgggtagatg ccgtgcagcg attcgtagtt gtggacggcc
     3301 aggatcagct ggctcaggtg attctggcac tggaaccggc gggccccctc gcgggccgac
     3361 tgcacggccg gcaagagcag cgcgaccagc gcgccgatga tgccgatcac cgccagcatt
     3421 tcgatgagcg tgaaggctcg caaacggcgg gtcatggagc gtcctccttc ggtgccagcg
     3481 gaacctcgcg atgcacagag gcccgctgcc aggggtcgtc cgggtagcgg gcctggacca
     3541 cgatcctcgc cggctgatcg gcgggccgct cgacgcgaat ctcggcgacg ccgacattcg
     3601 ctggcgtgac ttcgggccgc caggtctcgc cggaataatc cgcctgcgac cgcagtttca
     3661 ccaggccgcg ggccagggcc gcttcggcca gccactgggc ctgcacctcg tcgccggcgc
     3721 ggcgcgactg gcgatggctg ccaaccagcg agcgggccac cgtacccgcg atcagcatca
     3781 cgaccatcag agtgaccagg gcgatgacca gcgccgcggc gcggcgtttt atcttcacgg
     3841 cagcgcctcc ttttgctgca gcgccagggc ggcgtcgatg gtggttgtgg cgatgggcgg
     3901 tgagccggtc gccggggaac cgtcggggcg aatcgtcagc cgcacaaggc tgccgcctgg
     3961 ctgctcgact ttggcgaggt tcactgcagc gggagcaggc aggcgaaagg agtcacgatg
     4021 ctccaccgca tcgccgcggc ggacctcgcg ggtgatttcg cggtctccgg cggcatagcg
     4081 aaccgcgcgg ccatcgggcc aggtcaggac gcaatcctca ccgacggcgg cgtccttcgc
     4141 ctggtgaacg tcgccgcgga actgtgcgcc gagccgggcc agcatggtgg actgttccgc
     4201 gtcgcggcgg aactggcgct ccagcttcag cagcgccgcc agcgtcgtcg tgctggcagc
     4261 cagaacgacc gacaggatgg tgatgatgac catcacctcg actagcgttt tcgcccggcg
     4321 gaggttcgtc atggcgcggc ctccccgaat cgccagatag aaagccccac cggctccatg
     4381 ggttgcccca ccgaattcgt ccaggcgact tccaggcgga tcttgcgggc cggcgctgcc
     4441 ggctcctcgc tgacggcgat cgagcactgg gcggccggca ggacttcgct caactcggcc
     4501 gacggctgcc agccggtgag cttatcgggc gcggtctcgc tccagggcaa cagcgcgatc
     4561 cgctcggcct ggttggcgat ctcctgcaag gcgatgcggc gctgcgccat cgcgcggcgc
     4621 tggctggcgg ccagcgcgac gagctgcacc agcgccgcca tcgccacgcc cagcacgacc
     4681 aggctcacgg tcagttcgac gacggtcatt ccgcgttgtc gcgatcggct gttcatgcgt
     4741 gccaggtccc cccctccgtt actctatgcc aggccttgaa tgagcgaaat cagcggcagg
     4801 aacatgccaa tcgtaaacaa ggcagcgaac atgcccagga cgacaaggat tacaggaaac
     4861 aggacgttca caagggcctg aatcttgtgc gcctgccgac gcatggcgct gtcggccatt
     4921 tccgccagcc cccattcgag gttgcccgtc cgctcggccg ccgacaagag cgccgcgtcg
     4981 gtccagccga tcaatccagt ctgccgcagg gcatcgcacc agtcctgtcc ggcttcgacc
     5041 cgctcggctg cccgccgcaa gcggccggag acgatcgaca ggggatagca atcggcgacc
     5101 attcgcagcg cttccggcag cggacgtccc tggcggacag cgaggcccag gccgcgcatg
     5161 acgagcgccc cgtcgaaccg ccggaacagc agccaccacc cggggatgtt ccgcggcagc
     5221 cagccgatgt agtacagaat cgcgatcaga aagaacagcg gcatcgccag cgtcaggaaa
     5281 ggcagcagga acacccagcc gacttccacg aaccagctgg aggcattgac gacgagcctg
     5341 gtcatcgccg gcagcttcaa tccaaattct tcgaacatct tctggaacac gggcacgatc
     5401 ttgagcatcg taaaagtcgt cacgccgctg agcacccagg tcacggtgcc aagatagaaa
     5461 aaccgggcca gcgcggtttg cagcgccata tcgacttcgt gcgagttttc gagctgttgc
     5521 cgcatcgccg gtccgagcat ccccagccgc tcgcccagcc gcacggccag cttcatcgcc
     5581 gttcccagcc gcaggcgggc ggtgtggacg gcctgggcca ggggctcgcc gcgctcgatg
     5641 gcttcggcca gcgccagcga gcggacgccc gtgtcgccca gggtctcgtc ggcataggcc
     5701 cgcgcggtct cggacagcgg gatgcccttc tgggctgcgg ccgccagcgc ccagaccagc
     5761 gcccggtgct cggaatgccg cagcctgtcg ataatcatca ggagcagaac caccgcgatg
     5821 aaaggcaacg ggatgatcag gaaccagaag cccatgatgc cgaccagcaa tcccaggccg
     5881 gccatcagga acatgatcgt gctggccatc gacagcaagg actgcatcgg atcggcgacc
     5941 gccagccgcc gctggccata gagcgctcgc acggccaggc ggagggccag cgccggaatg
     6001 gccagcaaga cgagcgtcag cggtcccagc ggttcgttca cgtcggcctt tcgttagctc
     6061 aaaccttgaa tcaagctgat cagcgggagg aacaacgaaa tcgcgccgga ggcgatcgcc
     6121 atggtcacgg tgatgaacag gatcggcggc agcacctggc ccagcaggtt ggcccgcagg
     6181 ttgagccgcc cttcgagcat ttcggctgcc gagcggaggc ccaggccgag cgcgtcgtga
     6241 ttctcgcccc agtggaccag cggcacgatc gacagcggca gcgtccgcag cttcaccagc
     6301 gacatcgtga gcgaggtgcc ctgctcgacc tggcgggcga gcgcttggca ttggccgccg
     6361 acgtaggcgt cgctgatgcc gcccgcggtg aggcgcaaag cctcgggcag cggcacgcgc
     6421 tgctcgacga gcagtcccag gcagcggagc atctcggcca cgccggtcca gtgccaggcg
     6481 ttgccgatca gcggcagact ggaaatcagc cacgaccagc cggcccggcc gccaaacact
     6541 cgcgtggtga cggcggtggc cacgattcct acccccagga acgccagcag cggcggggcg
     6601 acctcgcacg accgcaggaa ttgcacggtc atcgccggaa gcctaagtcc aaattcctcg
     6661 aacatctgct tgaacggggg gacgacctcc accgcaaaca ggatatagac gccgatcgcc
     6721 atcagcagcg aaaagacggg ataggccagg gccgccacga ttgcccgcca gtgtgcccgg
     6781 gccgcccggc ggttctggag ccactccacg agcatggggg caatggcccc gctgcgctgc
     6841 gccgcctgaa tcaggccgac caggtgcggc ggcaggcggc gaatcgagat ggccaggcag
     6901 tcttcgagcg agcgaccgcg ctcgacctcg tttgcgattg acttcaggcc gcgcgacagc
     6961 cgccacgagt cgcactcggc ggccgacgcc cgcagacccg ccgccagcgg catgcccgcc
     7021 gcggtaatct gcgccgcgcg gtccagcacc gcttccgccg ccgcagcgga tagtacgggc
     7081 acgtcgctgg agtattgctc gtccacaatc gtgatcaata cggctgagcc aggcgcatca
     7141 tgatggcgat ccaagggccg agcgcgacaa acgcgtagac cgacgccacg gtaccgcaga
     7201 ggccgaccgt gagggccagc ggcacataaa ccgtcagcca ctggccgcgg cggtcgaatt
     7261 cttcccgata gacctccgcc gtgcgggcca gcgtgcggca gagctgcggc tgcgactggc
     7321 ccgaggcgac cgtccaagcc aatagcggcg agaaaccggc gggcggctcg tcgatccgct
     7381 caccgcggcg gagctgctgg gccaattgct tgccgccgcg agccagattg cccgagccgc
     7441 aggcgccagt ggccagctcc accgcttcgg ccagtggcac gctgtggtgc agcagcaaac
     7501 caagcagctc ggcgagcgac gccatccgcc cggcccgctg catgcgggct agcgtgccga
     7561 ctgcgccgaa ggccaacagc gggtgcagct cataaccgta cgccacgcgc cccgaacgat
     7621 accaggcgac gaccagccag acggtgatga gcgccggcac gagcgggccc cagacccagg
     7681 agctttcggc cgcccgatcg acccactggc ccaccggcag gcgggtgacg tcgaactcgt
     7741 cgagcatccg ggcaaagacc ggtgcgagct tggtgaggac aaacaggccc aggccccagg
     7801 tcagcagcag cagcacgacg ggataaatca gcgcgaggcc gatcgcacgc cgcagctggc
     7861 tgatccgccg cgccgtgcga gcgacacctt cgagggccgc gggcaatcgc ccggcacgca
     7921 gccccgccgc gatcagcgtt tgatacgcgg gcggcagcga gcttcccaac tcggcgacga
     7981 cctgcggcaa gggctggccc gcgtcgatcc gcttgcccat ctcgcccgcg aatcgcccca
     8041 atcggcccgg cagctcgcgg gccatttccc tcaggccctg atcgagcggc acaccggcgc
     8101 gaaccagcgc cccgatttcg tcagagagcg cagccagctg ctcgagcgtc agcggcggcc
     8161 tggcaagcgt agcggagatt gtcacaaccc gcagttccgg cgaagaggag attgcgccca
     8221 gactaaccga ctctctggcg gatcgcaaat cggccgtcaa tccactctga tcacgattcg
     8281 gaccggcgtc gtcagaggcg gcgtgccgtt gtcggtggct tccagaatca ggtgcagcgt
     8341 cttgccagcc gcgtccggcg ggatttccac cgtggcggcg gccgtcaact cgcttcgcac
     8401 cttgaccaaa cctcgataag ttccagcgtc cgcataaatc caccacttgt attgcacgcg
     8461 gtgtccatcg ggatcgctcg ccgacgcaga aactgtcagc ggctggccgg cgaccgcctt
     8521 gcggtcgagc gggcgagctg cggccgcgcc atcgatcacg acggctggcg catgattggc
     8581 gttcttggtt tcctggacgc accaatccat ccgggcctgg aagtcgcgct gccagatgtc
     8641 ccgccaccgc cagaccgccg cccgcacgtc gctctttccg tcaacgaaat cggccgcatc
     8701 gcgccataat cctcgctcgg cctgcttgaa ccgcccgcca aatccgcccc agccgggctc
     8761 gctcggatcc tgcaagccac tgggccgaaa gtagaaccac gaaggcgtgt ccccctcctt
     8821 cagcgcgctg tgcggattgg gcgcggtcca agtcttggcc ggatagagag cgcctagcgg
     8881 accatgattc tgccgtacgt gctcgtcgag ccacgcgagg ctggtcagcg attcgtcgcc
     8941 gcccaggtac atcccgcgat agacgccctc gcgcttgtcg cgcccgggct ctgcgctggc
     9001 cagcacataa aacaaatcgg ggaagttctc gaaaagccag gactgaattt tgtcctggtc
     9061 gttgatgtca tacacgcgca gctttttgat gaaggaccgt acgccgtccg ggccgcgatc
     9121 ttcgcgcacg cgccatagcg cctgcgcgaa atcggtttgg ccgccccaga tggtgacgtt
     9181 cagcggtcgc gaatcggccc ggtctgcggc gcggatgatc cacgccgagc cggcagtgtc
     9241 gcgtcccgcg ccgatctggg cgataccgcg ctcgcggttt cccgactgga tgcaggccag
     9301 caggttctcc acgggcggaa atcccgcagc atgcttcgac aagtgcggcc gaacttgaac
     9361 tcgttgctgt gca
//
LOCUS       DASRJB010000098         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1156309, whole genome shotgun sequence.
ACCESSION   DASRJB010000098 DASRJB010000000
VERSION     DASRJB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156309"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1156
                     /locus_tag="VFB80_02985"
     CDS             101..1156
                     /locus_tag="VFB80_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32748.1"
                     /translation="MMSLAGIALVYAPTRLEGLRRRWGTKGQAKFRLQQAHAHELALR
                     EGTTAAANQMQRGRKATVKKATAKQVAANQAHVQQQAEFEDYELEDQLQRDVIDELED
                     QLQFGLPVKRVERSFVPNFDFYGCEAVVVVGQDGLVANTAKYVGDLPIVAINPDPQRI
                     DGVLLPFQVKQARTIVRRVLDGKHKVRAVTLAEAELNDGQKLLAFNDFFVGAASHVSA
                     RYVLEAGGRSEPQSSSGILVSTGAGSTGWLSSVFNMAGGLAEFLGGEPLPRPQLAWED
                     RRLAWVVREPFLSKRSQASLVAGWLDEGEELIVESLMPDGGVIFSDGIESDFLPFSSG
                     AIARLRVSPQRANLVVG"
     gene            complement(1169..2353)
                     /locus_tag="VFB80_02990"
     CDS             complement(1169..2353)
                     /locus_tag="VFB80_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32749.1"
                     /translation="MQCIRLTRVLGLVAALALGSATIALACNVPVFRFALERWRPDPY
                     RFVLYHRGPLSAADLETIRPLEEQQDKALANVVLRAIDVAELDKPAAEGDEQSALDRR
                     LFAALGEVSLPRLAVHYPEHLRIDQPVWSGAPGREAIASLLDSPVRQELIKRLAEGQT
                     AVWLLLEPAAGGAASATLLETELNKLEQELKLPELTSAPEDTLLVNTPLKVDFSLLRV
                     RRDDPAEQELVAMLIHSEPDLAERSDAMVFPIFGRGRALLALIGAGITAKNIHDTASF
                     LVSACSCEVKELNPGFDLLLAADWEKRLSADGVPIAAPAAAAAPAGPPVLVPIPTGAA
                     QATVSSPTPVVEHPVIGVTFLNIRAWLIAGGSLLAALILVGAIVVFAQFRASSNLDDR
                     AR"
     gene            complement(2357..>4798)
                     /locus_tag="VFB80_02995"
     CDS             complement(2357..>4798)
                     /locus_tag="VFB80_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32750.1"
                     /translation="CFGAADQAPVAPHKHALAAQPLPADAAAKSKADSILRSSGVGEG
                     YCVVVGVGSATLITALAQQATQLRMIVIESDAHTANEFRGLLRISGIGAERVSVIVAD
                     PARVDLPPYLASLAIAEALPAAAPARAALMARLYTALRPFGGVACLPVPGEHAGSLAA
                     SLKEHGIAGADWTAADWKPAGDMTLLTRAGPLPDSADWSHEHADAANTRVSKDKRVKA
                     PLGLLWFGGPSNEAILPRHGHGPQPQVVEGRLFIEGVNILRAMDIYTGRLLWEASLPG
                     IGKHFDNTAHQPGANGSGTNYIATPSGIYVAYGRSCVVLDPATGQRTDEFRLPAEAGA
                     KEPPLWGYINVAGEFLVGGAEPLVDAGVVKSRGDNDDYSSSERLVVMDRSSGKVLWTA
                     TAKSGFRHNAICVGGGRLYCIDRLSGPELSRLKRRGETPANQPRLLVFDLASGRELWS
                     TEQDVFGTWLSYSAERDVLVEAGRPASDTISDEPKGMRTYHADRGGVLWENKSFSGPA
                     MLHHDTILMAGKACDLYTGAPRMREHPLSGEPVEWTWSRNYGCNTPLASEHLLTFRSG
                     AAGYFDMCNDGGTGNFGGFRSSCSNNLIVAGGVLTAPDYTRTCVCSYQNQTSIAMVHM
                     PEIETWTFFGAQTPKERVRSLGLNLGAPGDRKDERGTLWLEYPSVAGSSPAISVKIEP
                     EQPQWFRRHSSQISGPGLPWVAASGATGLTSIRIKLADPGSQPRAYTLRLHFAEPDAV
                     AAGERVFNVTVQGKPALTDLDVLKEAGGRYRAIVKAVAGVQVADELQIELAPAKDARI
                     QSSVLCGIEIQAEGW"
BASE COUNT          808 a         1558 c         1646 g          786 t
ORIGIN      
        1 tcgctggcga agaataaatg accgaagaac caaagaaccg aaaatccatg gtgatccgcc
       61 ctttggttct tcggatcttc ggttcttgga atttgctccc atgatgtccc tagccggaat
      121 cgcgctggtc tacgctccga cccggctaga gggcctgcgc cgccgctggg gcacgaaggg
      181 gcaggccaag tttcgcctgc agcaggcgca cgcacacgag ctggccctcc gcgagggaac
      241 tacggccgca gcgaatcaga tgcagcgcgg ccggaaggcc acagtgaaaa aggccacagc
      301 gaaacaggtc gcggccaacc aggcgcacgt gcagcagcaa gcggagttcg aagactacga
      361 gctggaggac cagctgcagc gcgacgtaat tgacgaattg gaggaccagc tgcagttcgg
      421 cctcccagtc aaacgcgtcg agcgctcgtt cgtgccgaat ttcgactttt acggctgcga
      481 ggcggtcgtc gtcgtcggcc aggacggcct ggtcgccaac acggccaaat acgtcggcga
      541 tctgccgatc gtcgcgatca atcctgaccc gcagcggatc gacggcgtgc tgttgccgtt
      601 ccaggtcaaa caggcccgga cgatcgtccg gcgcgtgctc gacggcaagc acaaggtgcg
      661 ggccgtgacg ctggccgaag cggagttgaa cgacgggcag aagctgctgg cattcaacga
      721 cttctttgtc ggggccgcgt cgcacgtctc ggcccgctac gtgctggaag ccggcggccg
      781 cagcgagccg cagtcgagca gtggcatcct ggtctccacc ggcgcaggtt cgacgggttg
      841 gctgtcgagc gtgttcaaca tggctggcgg actggccgaa tttctgggcg gcgagccact
      901 gccgcggccg cagctcgcct gggaagaccg ccgcctggcg tgggtcgtcc gcgagccgtt
      961 cctcagcaag cgctcgcagg cctcgctggt cgcgggctgg ctcgacgaag gcgaagaatt
     1021 gatcgtcgaa tcgctgatgc ccgacggcgg cgtcatcttc tcggacggca tcgagagcga
     1081 ctttctgccg ttcagcagcg gcgcgatcgc ccgcctccgc gtcagcccgc agcgggcgaa
     1141 cctggtagtc ggctgacggc cgatcgcgtc atcttgcccg gtcgtccaag ttcgatgacg
     1201 cgcggaactg ggcgaagacg acgatggcgc cgaccaggat cagcgccgcc aacagactac
     1261 cgccggcaat gagccaggca cggatgttga gaaaggtgac gccaatgacg ggatgttcca
     1321 caacgggagt cggagagctg actgtggctt gagccgcgcc ggttggaatc gggacgagca
     1381 cgggcggccc agcgggcgca gccgcggcgg cgggcgcggc gatcggcacg ccgtccgccg
     1441 agagccgttt ttcccagtcg gcggccagca gcaagtcgaa gccgggattc agctctttga
     1501 cctcgcacga gcacgcgctg accaggaagc tggcggtgtc gtgaatgttc ttggcggtga
     1561 tccccgcgcc gatgagcgcc aagagggcgc ggccgcggcc gaagatggga aaaaccatcg
     1621 cgtcgctgcg ctcggccaga tcgggctcgc tgtggatcag catcgcgacg agctcctgct
     1681 cggccgggtc gtcgcggcga acgcgcagca gcgagaagtc aaccttcagc ggcgtattga
     1741 ccagcagcgt gtcttcggga gcagaggtga gctccggcag cttgagctcc tgctcgagct
     1801 tgttcagctc cgtttccaag agcgtggcgc tggctgcgcc gcccgcagca ggctccagca
     1861 gcagccagac ggcggtctgg ccttcggcga gccgcttgat tagctcttgc cgaacgggcg
     1921 agtcgagcag gctggcgatc gcctcgcggc caggcgcgcc cgaccaaacg ggctgatcga
     1981 tgcgcaggtg ctctggatag tggacggcca gccgcggcaa cgacacctcg cccagggcgg
     2041 caaacagccg gcggtctagt gctgactgct cgtcgccttc ggccgccggc ttgtcaagtt
     2101 cggcgacgtc gatcgcgcgg agcacgacgt tggcgagtgc cttgtcttgc tgttcttcca
     2161 gcgggcggat ggtctccagg tcggcggccg agagtgggcc ccgatggtaa aggacgaaac
     2221 gatagggatc gggccgccag cgctccagcg cgaagcggaa caccgggacg ttgcaggcca
     2281 gggcaatcgt ggcactgccc agcgccaggg cagcgaccag gccgagtacg cgggtcaaac
     2341 gaatgcactg catgaatcac caaccttccg cctgaatctc gatgccgcat agcacgctgg
     2401 actggattct tgcgtccttg gcaggcgcaa gttcgatctg cagctcgtcg gcgacctgca
     2461 cgccggcgac tgccttgacg attgcccgat aacgcccacc ggcctcctta agcacgtcga
     2521 ggtcggtcag cgccggcttg ccttgcacgg taacgttgaa gacccgctcg ccggcggcga
     2581 ctgcgtccgg ttcggcaaaa tgcagccgca gcgtgtaggc ccgcggctgc gacccggggt
     2641 cggccagctt gattcgaatc gaggtcaagc ctgtcgcgcc cgaagccgcg acccacggca
     2701 ggccggggcc ggaaatctgc gagctgtgcc ggcggaacca ctggggctgc tccggttcga
     2761 tcttgacgct gatcgcgggc gacgagccgg ccacgctggg gtattcgagc cagagcgtgc
     2821 cgcgctcgtc cttgcggtcg ccaggagcgc cgagattgag ccccaggctc cgcacgcgct
     2881 cctttggcgt ctgtgctccg aagaaggtcc aggtctcaat ctccggcatg tgcaccatgg
     2941 cgatcgaggt ctggttttga taactgcaca cgcaggtccg cgtgtagtcg ggagcggtta
     3001 gcacgccgcc ggcgacgatc aggttgttgc tgcaactcga acggaagccg ccaaaattcc
     3061 ccgtgccgcc gtcgttgcac atgtcgaaat acccggccgc tcccgagcgg aatgtcagca
     3121 ggtgctccga ggccagcggt gtgttgcagc cgtagttccg cgaccaggtc cactcgacgg
     3181 gctcgccact gagcgggtgc tcgcgcattc gcggcgctcc ggtgtacagg tcgcaggcct
     3241 tgccggccat caggatcgtg tcgtggtgca gcatggcagg gccgctgaac gatttgttct
     3301 cccacagcac gccgccacga tcggcatggt acgtgcgcat gcctttgggc tcgtcgctga
     3361 tcgtgtccga tgccggccgc ccggcctcga cgagcacgtc tcgctcggcc gagtagctga
     3421 gccacgtgcc gaacacatcc tgctcggtgc tccacagctc cctgccgctc gccaggtcga
     3481 aaaccagcag ccgcggctga ttggccggag tctcgccgcg gcgtttgagg cgcgagagtt
     3541 ccggacccga gaggcggtcg atgcaataca gccggcctcc accgacgcaa atggcgttgt
     3601 gccggaagcc gctcttggcg gttgccgtcc agagcacctt gccgctactg cggtccatca
     3661 cgaccagtcg ctcgctcgac gagtagtcgt cgttgtcacc gcggctcttg acgacgccgg
     3721 catccaccag cggctccgcg ccgccgacga gaaattcgcc ggcgacgttg atatagcccc
     3781 acagcggcgg ctccttcgct ccggcttcgg ccgggagcct gaattcgtcg gtcctttggc
     3841 cggtcgctgg atcgagcacg acgcaggagc ggccgtaggc gacatagatg ccgctgggcg
     3901 tggcgatgta gttcgtgccg ctgccgttgg cccccggctg atgggcggtg ttgtcgaagt
     3961 gcttgccgat gcccggcagc gaagcctccc aaagcagtcg gcccgtgtag atatccatcg
     4021 cccgcaagat gttgacgcct tcgatgaaga gccggccctc gacgacctgc ggctgaggcc
     4081 cgtgcccgtg ccggggcagg atggcctcgt tggacgggcc cccaaaccag agcaatccca
     4141 gcggggcttt gacccgcttg tctttcgaga cgcgggtgtt ggcggcgtcg gcgtgctcgt
     4201 gactccaatc ggcagaatcg ggcagcgggc cagcgcgcgt gagcagggtc atgtcgccgg
     4261 cgggcttcca atcggcggca gtccaatccg cgccggcgat tccatgctcc ttgaggctcg
     4321 cggccagcga gccggcgtgc tcgcccggga ccggcaggca agcaacgccg ccgaacgggc
     4381 gcagcgcggt gtacagccgg gccatcagcg cggcacgggc gggcgcggcg gcgggcaagg
     4441 cttctgcgat tgcgaggctg gccagatagg gcggcagatc gactctcgct ggatcggcaa
     4501 cgatcaccga tacgcgctct gcgccaatgc cggagatccg cagtaggccg cggaactcgt
     4561 tggccgtgtg ggcgtcggac tcgatcacaa tcattcgcag ttgtgtcgcc tgctgcgcca
     4621 gcgcggtgat cagcgtggcc gagcccacgc caacgaccac gcaatatccc tcgccgacgc
     4681 cgctgctgcg gagaatggaa tccgccttcg acttcgcagc cgcgtcggcg ggaagcggct
     4741 gagccgccag cgcgtgcttg tgcggcgcga cgggggcctg atccgcagcg ccgaagca
//
LOCUS       DASRJB010000099         7207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3133119, whole genome shotgun sequence.
ACCESSION   DASRJB010000099 DASRJB010000000
VERSION     DASRJB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3133119"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1144
                     /locus_tag="VFB80_03000"
     CDS             131..1144
                     /locus_tag="VFB80_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32751.1"
                     /translation="MFRSLVGVFLVSAIAFPAAAQEQLKVGIIGLDTSHAIAFTKELN
                     SETAAADVAGCPVIAVYPKGSPDIESSVSRVPDYTVQIEKLGVKIVGSIDELLKQVDC
                     VCLETNDGRPHLEQALPVFQAGKPVFIDKPVAGTLADAIAIYKAAAKYNCPMFSSSSL
                     RYGKGAQELRSGALGKILGCDAYSPCSLEPTHPDLYWYGIHGCETLFTVMGPGCESVT
                     RVSTPDFDLAVGKWKDGRIGTFRGIRSKGGSAYGGTAFGEKGVREIGSYGGYRPLIVE
                     IVKFFKTKKSPIDPAETLDLYAFMEAADESKRKGGAPATIEEVMKKAEGEAEKRLAAL
                     GVK"
     gene            complement(1280..2470)
                     /locus_tag="VFB80_03005"
     CDS             complement(1280..2470)
                     /locus_tag="VFB80_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32752.1"
                     /translation="MIDCILARWRRFISLTLLIFSAAAGSQAAADETAAREALWQKLE
                     KHCQPPAEFAGKFGDYKSPLVFADGSRVKSPEDWARRRKEILDTWHERLGAWPPLVEK
                     PTVKTLETVERDGFSEQHVQVQISPEGQVADGYLLVPPGKGPFPAVLVPFYEPLTSIG
                     RGEKGRGTHDYGLQLVKRGFVTLSIGTPGSVENIGLDTRQLLVDAGKKQRRQPLTLLA
                     YVSANCHTALAQMPNVNPERIGIIGLSYGGKWSMFSSCLHDKFACAVWSDPGIVFNEK
                     NANVNYWEPWYLGYDPAVQRKEGVPSEAKPRTGLYKELVEQGEDLVSLHALMAPRPVL
                     ASGGTEDPPPNWQALNHLIAVNELLGHKNRVAMTARKTHVPTPEALEVELDFLEYWLK
                     HGGK"
     gene            2726..4045
                     /locus_tag="VFB80_03010"
     CDS             2726..4045
                     /locus_tag="VFB80_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32753.1"
                     /translation="MAYRLTIFIILLSGVPALAAPADIEPASASGSAAGDVAALAAQL
                     ASPQFRLRQQAFERLLDAGKDALGPVAEVARSPHREARSRALSILAALAAAKNEDLAA
                     AAFQTLRDLESNQDQTIVHEVRAALAEVRVARAYRAIAAIRAGGGMVSSALGDEPTAG
                     SDLRIEIGSVWKGGNAGLKALAELRTTTYLSLEGAPIDDAALAYVGQLESLEYLYLKQ
                     TRVRGSGLSQLKLSSLTHLSLRELPIDDAALASLPTLPLLAHLGLDHTQVTDAGLGEL
                     ARYPQLDTLWLDYAPITDAGLAKLAALPKLRTLCLTGTKATGPGLASLRDLRALHYVS
                     LKEVSLSPDTTRHLAKVEQLEILGLDHTNVNDEQLADLAPLVNLKTLWLSKTPVTDAG
                     IAHLAKLKSLQMVYFHGSQVTAEGRKALRDALPNCRVEPDDDRPGRP"
     gene            4095..7058
                     /gene="uvrA"
                     /locus_tag="VFB80_03015"
     CDS             4095..7058
                     /gene="uvrA"
                     /locus_tag="VFB80_03015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN32754.1"
                     /translation="MPDTLSPPNLAAATLGGDDAIRIRGARTHNLKNVALDIPRGRLV
                     VITGPSGSGKSSLAFDTLFAEGQRQYIESLSVQARQLFNQLERPDVDAIDGLSAVICI
                     DQRPGHPNPRSTVATSTEIYDYLRLLFARLGEVSCHQCGAPIRQQSLAQIEESLQALP
                     EGTKVMLLAPIVRGKRGDHAEALAAVRKSGLVRVRIDGQVYELDEVPPLDPRKSHTIE
                     AVVDRLVIRKGAGGRLAESLALAARHGEGVVIASYQSPGEPGGTKTNSNGAWSERQFS
                     TVYACPQCQTALTELEPRTFSFNSPYGACPHCQGLGVREEFDPELVLPDLSRSVREGA
                     VVPWRSEPRGSSPRPGEAEKRTEGEEEKDAGFVTRHSKKRNVAADPFPSVSDFLTNHG
                     ASDQTPLEQLPPDVFEKLLCGESKAKFPGLLTLLEAEYATATDARRKEELEAFRATVP
                     CTVCGGARLRPEALAVRLGGKNIHEVTSLPVDAAAEFFRCLRFASDDALIAEPLVKEI
                     VSRLSFLEKVGLGYLTLQRPTDTLSGGERQRVRLATGIGSGLTGILYILDEPSIGLHP
                     RDNARLIAALRELQEQGNTVLVVEHDEAMMRAADWLIDIGPGAGIHGGQVMSEGTPAD
                     VAADPQSITGGYLSGRLLIEMPAKRRRVAKTRAIVLEGASLNNLKNVTATIPLGVFVS
                     VTGVSGSGKSSLINETLTRAILRKLGGQSARPGPYTSLRGVSQIDKLIEIDQSPLGRS
                     PRSSAATYTGLFDEIRKVFAATKEARQLGFKASRFSFNVAGGRCEECQGHGQKKIEMN
                     FLPDLYVTCPACHGARFNQQTLAVKYRGKSIAQVLEMPVEEALNFFENIESIRRTLDS
                     LAGVGLGYLPLGQPATTLSGGEAQRVKLATELARTDTGQTLYVLDEPTTGLHFDDIRL
                     LLGVLHRLVDRGNTVLVIEHNLEVVKAADWVLDLGPGGGAAGGQIVAAGTPEEVAAME
                     ENFTGRFLRETLARS"
BASE COUNT         1317 a         2342 c         2296 g         1252 t
ORIGIN      
        1 aaacactcac caaacgcagc cctgccttca ttttgcaaat tgtccattgc aaattgaaaa
       61 cagtcgcgac gccgtgtttc ctcagttaca attccaccgg ttatctgcct ccgcctcgaa
      121 aggtcccgcc atgtttcgct ctctcgtcgg agtcttcctc gtgtctgcga tcgctttccc
      181 cgctgccgcc caggagcaac ttaaggtcgg catcatcggc ctcgatacgt cgcacgccat
      241 cgccttcacc aaagaactca acagcgaaac ggcggcggcc gatgtcgccg gctgcccggt
      301 gattgccgtc tatcccaagg gaagcccgga tatcgagtcg agcgtttccc gcgtgcccga
      361 ctacacggtg caaatcgaga agctgggagt gaagatcgtc ggctcgatcg atgagctgct
      421 caagcaggtg gattgcgtgt gcctggaaac gaacgacggc cgaccacacc ttgagcaggc
      481 gttgccggtg ttccaggcgg gcaagccagt gttcatcgac aagccggtgg ccggcacgct
      541 ggccgacgcg atcgcgattt acaaggcggc ggcgaaatac aactgcccca tgttctcgtc
      601 gtcttcgctg cggtacggga agggtgccca ggaactccgc agcggcgccc tcggcaaaat
      661 cctcggctgc gatgcctaca gcccctgctc gctggagccg acgcatcccg acctgtactg
      721 gtatggcatt catggctgcg agacgctgtt taccgttatg ggccccggct gcgagtcggt
      781 cacccgcgtt tcgacgcccg acttcgacct ggccgtcggc aagtggaagg acggccgcat
      841 cggcacgttc cgcggcattc gcagcaaggg cggcagcgcc tacggcggta ccgccttcgg
      901 cgagaaaggg gtccgcgaga tcggttccta cggcggctac cggccgctga tcgtcgagat
      961 cgtcaaattc ttcaagacca aaaagtcgcc catcgacccg gccgagacgc tcgatctgta
     1021 cgccttcatg gaagcagccg acgagagcaa gcgcaaaggc ggcgcgcccg cgacgatcga
     1081 agaagtgatg aagaaagcgg aaggcgaagc cgagaagcgg ctggcggcgc tgggggtgaa
     1141 gtgagcgaac ccggagatca ttggaacggg acacggaaaa cacggaagac acggactatc
     1201 taacgggccg atcattcagt tccgtgtatt ccgtgttctc cgtgtccgat cacaggcgct
     1261 acccactcaa gtgcaggctt catttcccgc catgcttcag ccagtattcc agaaaatcca
     1321 gctccacttc cagtgcctcg ggcgtcggca cgtgcgtctt gcgggccgtc atcgccacgc
     1381 ggttcttgtg gcccagtagt tcattcaccg cgatgaggtg attgagcgcc tgccagttgg
     1441 gcggcggatc ttcggtgccg ccggacgcca gcaccggccg cggcgccatc agcgcgtgca
     1501 ggctcaccag gtcttccccc tgctcgacca gctccttgta cagccccgtc cgcggcttgg
     1561 cctcgctcgg aactccctct ttccgctgca cggccgggtc gtaccccaga taccacggct
     1621 cccagtaatt cacgttggcg tttttctcat tgaacacgat gcccgggtcg gaccagacgg
     1681 cgcaggcgaa cttgtcgtgc aggcacgagg agaacatcga ccatttgcca ccgtacgaca
     1741 ggccgatgat gccgatccgc tcgggattca cattgggcat ttgggccagg gccgtgtggc
     1801 agttcgcgga gacataggcc aagagcgtca gcggttgccg ccgctgtttt ttgcccgcat
     1861 cgaccaggag ctgccgcgtg tccaggccga tattctccac cgagccggga gtgccgatgg
     1921 agagggtgac aaagccgcgc ttaacgagct gcagtccgta gtcatgagtc ccgcggcctt
     1981 tttcgccgcg cccgatgctg gtgagcggct cgtagaaggg aacgagcacg gcggggaacg
     2041 gccctttgcc gggcggcacg agcaggtagc cgtcggcgac ctgtccttcc ggcgaaatct
     2101 gcacctgaac gtgctgctcg ctgaagccgt cgcgctcgac ggtctccagc gtcttgaccg
     2161 ttggtttttc aacgagcggt ggccaggccc cgagccgctc gtgccacgtg tccagaatct
     2221 ccttccgccg ccgcgcccaa tcctcgggtg acttgactcg cgacccgtcg gcaaacacca
     2281 gcggcgattt gtagtcgcca aattttccgg caaactccgc cggcggctgg cagtgctttt
     2341 cgagcttctg ccacagcgcc tcgcgtgccg cggtttcatc ggccgcggcc tgactgcctg
     2401 ccgctgcgga gaagataagg agagtgagcg aaatgaagcg gcgccatcga gccaggatgc
     2461 aatcgatcat cggtttctcc tcgccagcgg aaggtcaggc cgccacgata acgcacgctg
     2521 cggccgccgg ctatctgagg gggtcgggag tctttttcgg ccgaagcctt tggtgatagc
     2581 cggacgatgg ccagaaaatg tctcccgacc cctttgggct agccccagcc accgctcgat
     2641 tctgcttgac cttgcgtcgc ctgcggcgca ccattccatt agcggtccac ctgtcgccgc
     2701 gacgttttcc gctgccgaga cccacatggc ctatcgcctg accatattca tcatcctctt
     2761 atcgggcgtc cccgctctgg cggccccggc cgacattgag ccggcctcgg cctccggttc
     2821 ggccgcggga gacgttgccg ccctggccgc ccagcttgcc agcccccagt tccggctccg
     2881 gcagcaggcg ttcgagcgac tgctggatgc cggcaaggac gcattggggc cggtcgccga
     2941 agtggcccgc tcgccccacc gcgaagcccg cagccgcgcc ctgtcgattc tggctgcgct
     3001 ggccgccgcg aagaacgagg acctcgccgc cgcggcattc cagacgctcc gcgacctgga
     3061 atcgaaccag gatcagacca tcgtccacga ggtccgcgct gcactggcag aggtccgcgt
     3121 ggcccgcgct taccgggcga tcgcggccat tcgcgccggc ggcggcatgg tgtcgtcggc
     3181 actcggtgac gagccgacgg ctggctccga cctgcgcatt gagattggca gcgtctggaa
     3241 aggaggcaat gccggcctca aggcactggc tgaattgcgg acgacgactt acctgtcgct
     3301 ggaaggcgcg ccgatcgacg atgccgcact cgcttacgtc ggccagctcg agagcctcga
     3361 atacctctac ctcaagcaga cgcgcgtccg cggcagtggc ctttcgcaac tgaaactctc
     3421 cagcctcacg cacttgtcgc tgcgggagct gccgatcgac gatgcggcac tggccagtct
     3481 gccgacattg ccgctgctgg ctcacctggg cctcgatcac acgcaagtga ccgacgcggg
     3541 gctgggggag ctggcgcgct atccgcagct cgacacgctg tggctcgact acgcgccgat
     3601 caccgacgcg ggcctggcaa aacttgccgc attacccaag ctgcgaacgc tgtgtctgac
     3661 cggcaccaag gcgaccgggc ccgggctggc cagcctgcgg gaccttcgtg cgctacatta
     3721 cgtttcgctc aaggaagttt cgctcagtcc cgacacaacg cggcacctgg ccaaagtcga
     3781 gcaactggaa atcctgggcc tcgatcacac gaacgtcaac gacgagcagc ttgcggacct
     3841 ggcgccgctc gtgaatctca agacgctgtg gctctctaag actccggtga ctgacgccgg
     3901 catcgcgcac ctggcgaagc tcaagtcgct gcaaatggtc tactttcacg gctcgcaggt
     3961 gacggccgag gggcgcaaag ccctgcggga cgcgctcccc aattgtcgcg tcgagcccga
     4021 cgacgaccgg ccgggaaggc cgtgaaggag gcggggagct tgtcgtctgc ggccaggaat
     4081 ctcaaattgg catcatgcct gatactcttt cgccgcccaa tctagccgcc gcgacactcg
     4141 gcggcgacga cgccatccgc atccgcggcg cgcggacgca caatctcaaa aacgtcgctc
     4201 tcgacatccc ccgcggccgc ctcgtcgtga ttacgggccc cagcggctct ggcaaaagca
     4261 gcctcgcctt cgacacgctt tttgccgaag ggcagcggca atacatcgag agcctgtcgg
     4321 ttcaggcgcg gcagctcttc aaccagctcg agcggccgga cgtcgatgcg atcgacgggc
     4381 tctccgccgt gatctgcatc gaccagcggc cgggccaccc taacccgcgg agcaccgtcg
     4441 ctacgtcgac ggagatttac gactacctgc ggctcctgtt tgcccgcctg ggagaagtct
     4501 cttgccacca gtgcggcgcg ccgatccggc agcagtcgct ggcccaaatc gaggagtcgc
     4561 tccaggccct ccccgagggg accaaggtca tgctgctggc gccgatcgtc cgcggcaaac
     4621 ggggagacca cgccgaagcg ctggccgccg ttcgcaaaag cgggctggtg cgggtccgca
     4681 tcgacggcca ggtctacgag ctggacgaag tcccgccgct ggatccgcgc aaaagccaca
     4741 cgatcgaggc cgtggtcgat cggctggtga ttcgcaaggg cgccggcggc cggctggccg
     4801 agtcgctcgc cttggcggcc aggcacggcg aaggagtggt gatcgccagc taccagtcgc
     4861 cgggcgaacc gggcgggacg aagacgaaca gcaacggtgc atggagcgag cgtcaattct
     4921 ccactgtcta tgcctgccca cagtgtcaga ctgcgctgac ggagctggag ccgcgtacgt
     4981 tcagcttcaa cagcccttac ggcgcgtgcc cgcattgcca ggggcttggc gtgcgcgagg
     5041 agtttgatcc ggagctggtg ctgccggatt tgtcgcgctc ggtccgcgag ggggcggttg
     5101 tgccgtggcg tagcgagccg cggggttcat ccccgcggcc cggagaggca gagaagagga
     5161 cagaaggaga ggaggagaag gacgcgggtt ttgtgacgag acacagcaag aaacgcaatg
     5221 tcgcggccga tccatttcct tccgtaagcg actttcttac caatcacgga gccagcgatc
     5281 agacccccct cgaacaattg ccgccggacg tgttcgagaa gctgctctgc ggcgagtcga
     5341 aggcaaaatt ccccgggctg ctcacgctgc tggaggcgga atacgccacg gcaaccgacg
     5401 cgcggcgcaa agaggaatta gaagcctttc gcgccacggt cccttgtacc gtctgcggcg
     5461 gggcccggct gcggcccgag gccctggccg tgcggctcgg cggaaagaac atccacgagg
     5521 tgacgtcgct gccggtcgat gcggccgccg aattcttccg ctgcctgcgc tttgccagcg
     5581 acgatgccct catcgcggag ccgctcgtca aggaaatcgt cagccggctg tcgtttctcg
     5641 aaaaggtcgg cctgggttat ctcaccctcc agcgccccac cgacacgctc tccggcggcg
     5701 agcgccagcg cgtccgtctg gccaccggca ttggctcggg actcaccggc attctttata
     5761 ttctcgatga gccgtcgatc ggcctgcacc cgcgcgacaa cgcccgcctg atcgcggcgc
     5821 tccgcgagct tcaggagcaa ggcaatacgg tgctggtcgt cgagcacgac gaagccatga
     5881 tgcgcgcggc cgactggctg atcgacatcg gccctggcgc cggcattcat ggcggccagg
     5941 tgatgagcga aggaacgccg gcagatgttg ccgccgatcc gcagtcgatc accgggggct
     6001 acctttccgg gcgcctcttg atcgaaatgc cggccaagcg ccgccgcgtg gccaaaacgc
     6061 gggccatcgt gctggaagga gcgtcgctca acaacctgaa aaacgtcacg gccacgattc
     6121 cgctgggcgt gttcgtgtcg gtcaccggcg tgagcggctc cggcaaaagc tcgctgatca
     6181 acgagacgct gacgcgggcc atcctccgca agctcggcgg ccaatccgcc cggcccgggc
     6241 cgtacaccag cctccgcggg gtgagccaga tcgacaagct gatcgaaatc gatcaaagcc
     6301 cgctgggacg ctcgccgcgg agcagtgccg ccacgtacac cggcctgttc gacgaaatcc
     6361 gcaaggtgtt tgccgccacc aaggaggcgc ggcagctcgg cttcaaggcc agccggttca
     6421 gcttcaatgt cgccggcggc cggtgcgagg aatgccaggg acacggccag aaaaagatcg
     6481 agatgaattt cctgcccgat ctgtacgtca cctgcccggc ctgccacggc gcacgcttca
     6541 accagcagac gctggcggtc aagtatcgcg gcaaaagcat tgctcaggtg ctggaaatgc
     6601 cggtggaaga ggcgctgaat ttttttgaga acatcgaatc gatccggcgg acgctggaca
     6661 gcctcgccgg cgtgggactg ggatacttgc cgctggggca gccggcgacg acgctttccg
     6721 gcggcgaggc gcagcgagtc aagctggcta ccgagctggc ccgcaccgac accggccaga
     6781 cgttgtacgt cctggatgag ccgacgaccg gtttgcattt cgacgacatc cggctgctgc
     6841 tcggcgtgct tcaccggctg gtagaccgtg gcaatacggt gctcgtgatc gagcacaacc
     6901 tggaggtggt gaaggcagcc gactgggtgc tcgaccttgg tcccggcggc ggcgctgcgg
     6961 gcgggcagat cgttgccgcg gggacgccgg aggaagtggc ggcgatggag gagaatttca
     7021 ccgggcggtt cttgcgggag acgcttgcgc ggtcgtgagg gggagaaaga gtaggggtag
     7081 gataaagatt aggaagagga ttaggagcag gagcaagagt aagagtaaga gtaagagaag
     7141 gaatgaggcg gctgaatttc ctaatctttc tcttactcca aatcttactc ttcttcctac
     7201 tcttaat
//
LOCUS       DASRJB010000100        11567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_878173, whole genome shotgun sequence.
ACCESSION   DASRJB010000100 DASRJB010000000
VERSION     DASRJB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878173"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFB80_03020"
     CDS             <1..304
                     /locus_tag="VFB80_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32755.1"
                     /translation="GQPLVGALVEFETTVDGKRVTAHGETQEDGSYKLESPGLGEGAV
                     AGQHKIIVSPPGREPGGRPPPAFDRRFQSYATTPLSFTVKPEGPFVYDFEVTAPRR"
     gene            complement(301..912)
                     /gene="rdgB"
                     /locus_tag="VFB80_03025"
     CDS             complement(301..912)
                     /gene="rdgB"
                     /locus_tag="VFB80_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZN32756.1"
                     /translation="MPGLLVLGSHNRKKLGELAELLAPHGLELKTLADFPAAIAVEET
                     GQTFAENARLKATQQARHLKAWVLGEDSGLSVDALGGAPGVLSARFSDPGATDERNNQ
                     LLLQKLASVPLEKRTAHYTCYAALSDPDGNLRAESEGICRGRILLSAAGSGGFGYDPL
                     FEIVECHRTFGELSPAVKSVLSHRSRAIRQIVPKIVALVAGPS"
     gene            complement(976..2106)
                     /locus_tag="VFB80_03030"
     CDS             complement(976..2106)
                     /locus_tag="VFB80_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32757.1"
                     /translation="MLRANWLTAVLLLVTGLAASWPASAQAAPPWATLIPFKKIEADP
                     NKSYELEEKHGPWIIMAASFAGAAAEQQAHDLVMELRQKFKLEAYTFRQSFDFSKPVE
                     GNGLSKYGGRRRMRNLYNNRFDEIAVVVGNFQTLDDPQLDKTLERLKYARPQVFQREE
                     NKSAQRMNTIRDFYRVVVNDPSRKSKGPMGAAFATRNPLLPKELFTAKGLDPFVAGMN
                     RDLPHSLLNCPGRYTVRVATFRGVDTMKPAEFERLTTQERKYSKIDEAALKASELCKQ
                     LRQQGVEAYEFHDRTESIVTIGSFAEVGQPRIDGKIEINPAIHRIMEAYGPIKQLKPG
                     TNMEEVYARVQNGLRFDPQPLPVEVPKQSIAAAYNPENSPYR"
     gene            complement(2179..4104)
                     /locus_tag="VFB80_03035"
     CDS             complement(2179..4104)
                     /locus_tag="VFB80_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32758.1"
                     /translation="MVGRAFLGIGSLLLCVGGTSWGQQPTPTPLPSLADAARAAAANF
                     KPVQASEVTAARSVLAATVSDLDRFLLTGASYKRPRWQTYLQWNDLVGLLQQDPPSPE
                     TSAAVLAKFRANVKGLELPQFANVATALERYMAASAAATSSNLKDEYAKQMVDLVTHL
                     EAYEKDPTAGDAALAIGRSLAWLERNGQAAPLIADIRRTYGKQNLFGYASKRLAAAGI
                     DGDVSQLTAVHDNILGTSLHGTAHLIGRTSVVMNENPRAASLTILLGGTAYSNTVGYN
                     GPVTIYSNGATNISGQKTIEMTAAGLIGYRALASAATNSRINAIQAKHAFVERIAWKR
                     AGQQKGQTEAIASQRAAGRAAGQMDSEAGRMVAEQNARYVEKFRDPLLKRGEFPEEML
                     FSSTSEQAQVRVLQASPALLAAPTVPPEQSADFDLALRTHESTVTNFGQGLLGGYELT
                     DLRLEKLIKDDLKGELPDELRVTLPDGTLDETKDPWSILFAKELPVRAKFSGGGVWIA
                     IRADGFTRGEGDTQGKYKPALTELVEISANYKIEKTEKGATLRRDGDVQVRFPARANP
                     EQITLRDSPIVTFIRRKFRSMFKEEFVGEGLALKGRWEKAGKLQLQEVQSDKAWLALG
                     WKLPAQAPPPPAAAGAE"
     gene            4379..5212
                     /locus_tag="VFB80_03040"
     CDS             4379..5212
                     /locus_tag="VFB80_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32759.1"
                     /translation="MAILLAAPRLSRGQIPIWYGDYAAAYKAAASDHRLLLLWFCDPE
                     QADANQRIETGVLGQSSIAAAIERRYVAAKLPLDAAFESESGLQRLLDHPSLAEMRGQ
                     SGLAVVDLTDPESPHYRLVVSVYPLARGPLSAEKLAVLLELPRGSLTQRTLIFAVRTH
                     PERPASTDGHLSPLLAGEAESHAAHQAAIHLQGHHHWEQRFHSINAQLKGGLLAQEIC
                     AESWPGQHLVEAAEECVDSWRQSPGHWDAVSRRHALFGYDMQRGANGVWYATGIFARQ
                     N"
     gene            complement(5230..7077)
                     /locus_tag="VFB80_03045"
     CDS             complement(5230..7077)
                     /locus_tag="VFB80_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32760.1"
                     /translation="MPPRSAWVRGFLLLALLLAGGCYQVPPPPPPPVPPVVATPVVAP
                     PVAASSDEPEDETPAIVETPPAPPPPTFARERIVLIAPVNPIIIEFQLSIDGQPHTAA
                     LTRLVTEVLALVDPDRDGRVTWKELCASKRVKYGQFGNLAIEGDNAEKQVIERYDIDK
                     DGVVDDTELPRFLTRNAGGSRPFSIRGNVDYRGSSARAAPTWQVIDADGDGAISAAER
                     AAAAAELIARDTNDDEILRSGDLNPRLLTADPEMMSERRRAGPDPAARLLGPHADWSD
                     VQRSLEQRYAGSSLLREGCFPLTPDLFAQLDANKDGRLGRGEFEKLNEVEPHLVVAVE
                     FGRPAANLLSQETDEPWARDREPIDGPAKKEPVKTEPRLRIVRVAPALAGDSPQIVEQ
                     SGRLTLTIGGVLLTLYTNDAVANEDFAARAAQAIAMYDNNKDGYLVKEEVPETLQAQF
                     GRFEAIDTDEDGKAFAGEIEEFLTQQQAGLRSQVHARASDREDPLFAALDADRDERLD
                     SREIEAASQRLASLDKNGDGEVTADELPELMVIGLARGSLENAEATFTPPPVISRGPA
                     GDAPRWFAAMDANGDGAISRREFLGESEKFAQLDKDGNGLLELHEAMNP"
     gene            7838..8929
                     /locus_tag="VFB80_03050"
     CDS             7838..8929
                     /locus_tag="VFB80_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32761.1"
                     /translation="MSPANQKQSRGFTLVELLVVIAIIGVLVALLLPAVQAAREAARR
                     MKCVNHLKQFGLALHNYADVYGVFPARRYGTTSTVASVWLHNNVRINAHVALLPYIEQ
                     GNMFNRIQAGDMTRTPDPIGPGGPRGDQNWAVWNAPPAILRCPSDAGAPRHLRNHSYV
                     FSAGDQVANVNTITLSRGLFGRTSWRRFAEISDGTSNTIAMSEILSHGPEFGGQFGLT
                     STAGQVKIGLGLANNIPGVVNSPSVCRTVSNGTHYVAGTNVRGRRGINWTDAPATLCM
                     FTTVLPPNSPTCAEGGDHGDQDNIVLPPVSSHPNGVNGVMCDGSVRFITNNINTGNLG
                     IRQPQDGGSVYGVWGAMGSIAGGEASPTE"
     gene            8940..9404
                     /locus_tag="VFB80_03055"
     CDS             8940..9404
                     /locus_tag="VFB80_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN32762.1"
                     /translation="MKRFATLLAIAAISAGCYGSGRPPTYPVSGTVTWQGKAVEGARV
                     VFVPTGSQESAAGITDAAGKYQLTTFVAGDGAEPGEYRVKVAKYDIKQPTKEELEKFI
                     TAEEEQKIVFAQDEKPTPPAKNLMPKKYESEITSGITHTVTTSPTTLDIKVE"
     gene            complement(9425..9727)
                     /locus_tag="VFB80_03060"
     CDS             complement(9425..9727)
                     /locus_tag="VFB80_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32763.1"
                     /translation="MGWAAVYIEQLRAGQTVSFRPRGNSMAGKIESGQLCTVAPVDPA
                     TLQVGDIVLCKVNGREYLHLVKARQGPRFQIGNNRGRINGWITASAIYGKCIRIEA"
     gene            9912..10526
                     /locus_tag="VFB80_03065"
     CDS             9912..10526
                     /locus_tag="VFB80_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32764.1"
                     /translation="MPSRCRLWFAACVVAGLLLSASSAQAQSSWSFSIGTGGYGHHHH
                     CHDGWCGPYWGPYYGPGFGVVYAAPPPVVRERVVYVQPQQPAWTAPANVTAAPTANAL
                     PANAAEDDRIVIRNMAGARLPVAFMVDGQDVELTDGATRTFVGRTRRMVTYDRGGRYG
                     STEQQLTAGHYEFRITPSGWDLVRRPDVAPASRTAVRSNALPER"
     gene            10655..11359
                     /locus_tag="VFB80_03070"
     CDS             10655..11359
                     /locus_tag="VFB80_03070"
                     /inference="COORDINATES: protein motif:HMM:NF039735.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HZN32765.1"
                     /translation="MRLSAIVLLAGILAGCGPSATQKKLEQYFVDWLKNHGEKQVVVD
                     SGGVGLAGNATRLKASIYGSKRHDGGHSVETEFRVKLPGGGEIVEFVAGMGDTHEAAI
                     DDSLVNFTLSTYHVVYKCFLNDADPHQPLKKLAIGGVERDVAFGDLFVRGSAGEKHNL
                     GELQPAIEQAIAGLSLGDGPHWIKIVYGQANGEVIVVAATLDNGDANALTETVRQMNW
                     PKSESFYMAKQFIVIK"
BASE COUNT         1882 a         3813 c         3799 g         2073 t
ORIGIN      
        1 gggacagccg ctcgtgggtg cgctagtcga gttcgagacg acggtggacg gcaagcgggt
       61 gacggcccac ggcgaaacgc aggaggacgg cagctacaaa ctcgagtcac ccggcctggg
      121 cgagggcgcg gtcgccgggc agcacaagat catcgtctcg ccgccgggcc gggagccggg
      181 cgggcggccg ccgccggctt tcgaccgccg gtttcagagc tatgcgacga cgccgctcag
      241 cttcacggtg aagcccgaag ggccgttcgt ctacgacttc gaagtcaccg cgccgcggcg
      301 ctagcttggg ccagccacca gtgcaacgat ctttggcacg atctgccgga tggcccgcga
      361 gcggtggctc agcaccgact tcaccgcggg cgagagctcg ccgaatgtgc ggtggcactc
      421 gacgatctcg aacaagggat cgtagccgaa gccgccgctg ccggcggccg agagcagaat
      481 cctgccgcgg cagatgccct cactttcggc ccttaagttg ccgtcgggat cggaaagcgc
      541 ggcgtagcag gtgtagtgcg ccgtgcgttt ctccagcggc acactcgcca gtttttggag
      601 cagcaattga ttgttccgtt catccgtcgc gcccggatcg gagaaccgcg ccgagagcac
      661 gcccggcgcg ccgccgaggg cgtccacgga cagcccgctg tcctcgccca gcacccaggc
      721 cttcaagtgc cgcgcctgct gcgtggcctt gagtcgcgcg ttctcggcaa atgtctggcc
      781 ggtctcctcc acggcgattg ccgcgggaaa gtcggccagc gtcttcagct ccaggccgtg
      841 cggagcgagc agctcggcca gttcgccgag cttcttgcgg ttgtgcgagc cgagcacgag
      901 cagtccgggc atgagagccg cctttccgcc gagagtttcc gcgcggcgga gcggcccgtc
      961 aagcggccgc agcgcttacc ggtacggcga attctccggg ttgtaagccg cggcgatcga
     1021 ctgcttgggc acctcgaccg gcagcggctg cggatcgaac cgcaggccgt tctgcacccg
     1081 ggcatacacc tcttccatgt tcgttcccgg cttcagttgc ttgatcgggc cgtaggcttc
     1141 catgatccgg tggatggccg gattgatctc gatcttcccg tcgattcgcg gctggccgac
     1201 ctcggcgaac gaaccgatgg tgacgatgct ctccgtacgg tcgtggaact cgtaggcctc
     1261 gaccccctgc tgccgcagct gcttgcagag ctccgaggcc ttgagcgccg cttcgtcgat
     1321 cttcgaatac tttcgctcct gcgtggtcag ccgctcgaac tcggcgggct tcatcgtatc
     1381 gacgccgcgg aaggtggcga cgcggacggt gtagcggccc gggcagttga gcaggctgtg
     1441 cggcaggtcc cggttcatgc cggcgacgaa cgggtcgagg cccttggcgg tgaacagctc
     1501 cttgggcagc agcggattgc gggtggcgaa cgcggcgccc atcgggccct tgctcttgcg
     1561 cgagggatcg ttcacgacga cgcggtagaa atcgcggatc gtattcatcc gctgcgcgga
     1621 cttgttctct tcgcgctgaa agacctgcgg gcgggcgtac ttcagccgct ccagcgtctt
     1681 gtcgagctgc gggtcgtcga gcgtctggaa gttgccgacc acgacggcga tctcgtcgaa
     1741 ccgattgttg tacaggttcc gcatccgccg ccgcccgccg tacttgctca ggccgttccc
     1801 ctcgaccggc ttgctgaagt cgaacgactg gcggaaggtg taggcctcca gcttgaactt
     1861 ttgccgcagc tccatgacca ggtcgtgggc ctgctgctcg gcggctgccc cggcgaacga
     1921 agcggccatg atgatccacg gcccgtgctt ctcttccagc tcgtagctct tgttcgggtc
     1981 ggcctcgatc ttcttgaacg gaatcagcgt cgcccagggc ggcgcggcct gcgcgctggc
     2041 cggccagctc gccgccagac ccgtcacgag aagcagcacc gccgtcagcc agttcgcgcg
     2101 cagcatgtcc gactcctttc gccaggggca agggtgcgga ccgtatcaaa ttcacgagcg
     2161 ggcggccaga gcgattcgtc actccgcgcc agcggcggca ggcggcggcg gtgcttgcgc
     2221 cggcagcttc cagccgagcg ccagccaggc cttgtcggat tggacttcct ggagctgcag
     2281 cttgcccgcc ttttcccagc ggcccttgag ggccaggccc tcgccgacga attcttcctt
     2341 gaacatgctg cggaacttgc ggcggatgaa ggtcacgatc gggctgtcgc ggagcgtgat
     2401 ctgctccggg ttggcccgcg ccggaaagcg gacctgcacg tcgccgtcgc gccgcagcgt
     2461 cgcgcccttc tcggtcttct cgatcttgta gttggcggag atttcgacca gctcggtgag
     2521 ggccggcttg tacttgccct gcgtgtcgcc ttcgccgcgc gtgaaaccgt cggctcggat
     2581 ggcgatccac acgccgccgc cgctgaactt cgcccgcacc ggcaattcct tggcgaagag
     2641 gatcgaccag gggtctttgg tctcgtcgag cgtgccgtcg ggcagcgtca cccgcagctc
     2701 gtcgggcagc tcccctttca ggtcgtcctt gatcagcttc tccagccgca ggtcggtcag
     2761 ctcgtagccg cccaggagtc cctggccgaa gttggtgacg gtcgattcgt gggtccgcag
     2821 cgccaggtcg aagtcggccg actgctcggg cgggacggtc ggcgcggcga gcagcgcggg
     2881 cgacgcctgg agcacacgca cctgggcctg ctcgctggtc gagctgaaca gcatttcctc
     2941 ggggaattcg ccgcgcttga gcagcgggtc gcgaaacttc tcgacgtagc gggcgttctg
     3001 ctcggcgacc atgcggccgg cttcgctatc catctggccg gcggcgcggc cggcggcgcg
     3061 ctgcgaagcg atggcctcgg tctgcccctt ttgctggccg gcccgcttcc aggcgatccg
     3121 ctcgacgaag gcgtgcttcg cttgaatggc gttgattcgc gaattggtcg cggccgaggc
     3181 cagcgcgcgg tagccgatca gccccgcggc cgtcatctcg atcgtctttt gccccgagat
     3241 gttcgtcgcg ccgttgctgt agatcgtgac cgggccgttg tagccgacgg tgttcgaata
     3301 ggctgtgccg ccgagcagga tcgtcaggct ggccgcccgc ggattctcgt tcataacgac
     3361 cgacgtgcgg ccgatcaggt gcgccgtgcc gtgcagcgag gtgccgagga tgttgtcgtg
     3421 caccgcggtg agctggctca cgtcgccgtc gatgcccgcc gcagccagcc gcttcgaggc
     3481 atagccgaac aggttctgct tgccgtaagt ccgccgaatg tcggcgatga gcggcgccgc
     3541 ctgtccgttg cgctccagcc aggccagcga gcggccgatc gccagagccg cgtcgcccgc
     3601 ggtcggatcc ttttcatacg cttccaggtg agtcaccaaa tccaccatct gtttggcgta
     3661 ttcgtccttc aagttgctgc tggtcgctgc agcgctcgcg gccatgtacc gctccagcgc
     3721 ggtcgcgacg ttggcaaact gcggcagctc caggcccttg acgtttgcgc ggaacttggc
     3781 gagcaccgcg gccgacgtct ccggcgaagg cgggtcctgc tggagcaagc cgaccaggtc
     3841 gttccactgc aaataggtct gccacctggg ccgcttgtag gacgccccgg tgagcagaaa
     3901 ccggtcgagg tcactcacag tggcggcgag gacgcttctc gccgcggtca cttcgctggc
     3961 ctgcaccggc ttgaagttcg ccgccgcagc gcgggccgcg tccgccagcg aaggcagcgg
     4021 cgtcggcgtg ggctgttgcc cccacgacgt tcctcccacg cagagtaaca gcgacccgat
     4081 ccccaagaat gcccgcccga ccatattccc gctccttgct tgagataaga aagtcggcga
     4141 ctgtggattc cttcgcaggg ctgcccggct cgattggccg ctgactagca cgcagaaaag
     4201 agtgcctttt gccccatctt ccgccgaatc gctgttttat gcaagcgaaa gattctgcct
     4261 gatcggacta cttgacgcca tcggaacaat cgtcctatgt tgtgcccgca aaagtcgtca
     4321 tagccctcgc aatctgctcc gtttttccat gtttcacagg ctgctaactc tcgctgtctt
     4381 ggctattctt ctggccgcgc ctcggctctc caggggacag atacccattt ggtatggcga
     4441 ctatgcggcg gcgtacaaag cggcggcctc cgaccaccgg ctactgctcc tctggttctg
     4501 cgatccagag caagcggacg caaaccagcg catcgagact ggcgttctcg gtcaatcgtc
     4561 catcgccgcg gcgatcgagc ggcgctatgt ggcggcaaag ttgccgctcg atgcggcgtt
     4621 cgaatcggag agcggcctgc agcgcttgct cgaccaccca tcgctggccg aaatgcgcgg
     4681 gcaaagcggt ctggcagtcg ttgatctcac cgacccggag agcccgcact atcgcctggt
     4741 cgtttcggtc tatccgctgg cgcgcgggcc gctctccgcc gagaagctcg cggtgctgct
     4801 cgagttgccg cgcggctcgc tgacgcagcg gacgctgatc ttcgccgtac ggacgcatcc
     4861 cgagcggccg gcgagcaccg acgggcacct cagcccgctg ctggcgggcg aggccgagag
     4921 ccacgcggcg catcaggcgg caatccatct gcaagggcac catcactggg agcagcggtt
     4981 tcactcgatc aacgcgcaac ttaagggcgg cctgctcgcc caggaaatct gcgccgagag
     5041 ttggccgggc cagcacctgg tcgaagcagc cgaggaatgc gtcgatagct ggcggcaatc
     5101 gcccggccac tgggacgccg tcagccggcg gcacgcgttg tttggctacg acatgcagcg
     5161 cggggccaac ggcgtgtggt acgcgacggg gattttcgcg cgtcaaaact gacggcgatt
     5221 ccccgcgcgt tatggattca tcgcttcgtg gagctcgagc aggccgttgc cgtctttgtc
     5281 gagctgggcg aatttctcgc tctcgccgag gaactcgcgg cgactaatcg cgccgtcgcc
     5341 gttggcatcc atcgcggcga accaccgcgg ggcgtcgccg gccgggcccc gggaaatcac
     5401 cggcggcggc gtgaacgtgg cctccgcatt ctccaggctg ccgcgggcca ggccgatcac
     5461 catcagctcc ggcagctcgt cggccgtcac ctcgccgtca ccgttcttgt ccagcgacgc
     5521 gagacgctgc gaggccgctt cgatctcgcg gctgtcgagc cgttcgtcgc ggtcggcgtc
     5581 gagggcggca aacagcggat cttcgcggtc gctggcccgg gcgtgaacct gggaccgcag
     5641 gcccgcttgc tgctgcgtca gaaactcctc gatctcgccc gcgaacgcct tgccgtcctc
     5701 gtcggtgtcg atcgcttcga agcggccgaa ctgggcctgc agcgtctcgg gcacctcctc
     5761 cttcacgaga tagccgtcct tgttgttgtc gtacatcgcg atggcctggg cggcgcgggc
     5821 ggcaaaatcc tcgttggcga cggcgtcgtt cgtatagagc gtcagcagca cgccgccgat
     5881 ggtcagcgtc aggcggccgg actgctcgac gatctgcggc gagtcgcccg ccagggcagg
     5941 cgcgacgcgg acgatccgca gccgcggctc ggtcttcacg ggctccttct tggccgggcc
     6001 gtcgattggc tcgcggtcgc gggcccacgg ctcgtcagtt tcctgcgaca gcaggttcgc
     6061 ggccggcctg ccgaactcga cggcgaccac gaggtgtggc tcgacctcgt taagcttctc
     6121 gaattcgccg cggcccaggc ggccgtcctt gttggcgtcg agctgcgcga acaagtcggg
     6181 cgtcagcggg aagcagccct cacggagcag gctcgagccc gcgtaacgtt gctcgagcga
     6241 gcgctgcacg tcgctccaat cggcgtgcgg cccgagcagc cgagcggcgg gatccggccc
     6301 cgcgcggcgg cgctcgctca tcatctccgg atcggctgtc agcagccgcg ggttcaggtc
     6361 gccgctccgc aggatttcgt cgtcgttggt gtcgcgagcg atcagctcgg cggctgccgc
     6421 ggcgcgttcg gcggcggaga tcgcgccgtc gccgtcggca tcgatcactt gccaggtcgg
     6481 cgcggcgcgg gcgctagaac cgcgataatc gacgttgccg cggatcgaga acggccgcga
     6541 gccgccggcg ttgcgggtca aaaaccgcgg cagctcggtg tcgtccacga cgccgtcctt
     6601 atcgatgtcg tagcgctcga tgacttgctt ctcggcgttg tccccctcga tcgcaaggtt
     6661 gccgaactgg ccatatttga ctcgcttgct ggcgcacagc tccttccaag tcacgcgccc
     6721 gtcgcggtcc ggatcgacca gcgccagcac ttcggtcacc aggcgagtca gtgccgcggt
     6781 gtgcggctgg ccgtcgatcg agagctgaaa ttcgatgatg atcggattca cgggcgcgat
     6841 gaggacgatc cgctccctgg caaacgtcgg aggcgggggc gcgggcggcg tctccacaat
     6901 ggccggcgtc tcgtcctccg gctcgtcgct agacgcagcg accggcggcg caacaaccgg
     6961 cgtggcaacg acgggcggaa ccggtggcgg cgggggcggc ggcacctgat agcagccgcc
     7021 cgccaggagc agcgctagca acaggaatcc acggacccaa gcgcttcgag gtggcatgag
     7081 actgcctccg gcgggacggc acccaagaca accgccccgg ctgacgattc cggcattgta
     7141 ttcgatcaaa agggctgatg ctcttggccg ggcctccggc aatataacga aaactcgagt
     7201 cacagctggc gtttggcgaa gcactgttgg ccgcggcccc gttcgcaact cgttactccg
     7261 gcgtctgttg cgtcggtttt ttgtcctcct gatcgcatta gagtttgaag ggtttgaagc
     7321 gcttgtcggg aattatcgtt atcgcgctgg cgctaggttg cagcgggtga gttcgcacac
     7381 caagggcgcg tcagcgaggg tgggtcagcg ccctcgccac ttcctcgccg gcatgtgggg
     7441 cgggcatgtc cgccttcgtc cacccagcac tgattcgctc gagatcttcc tatattgcca
     7501 caagaggaca ttatatcaca ttcgtccccg ttttttgtcg tttgggctgc aatctggatg
     7561 gagaatcgcg gcgcaacatc ttggctggct tgggcttgca acaattctca agaaaatgga
     7621 cgcgtaatcg agctttctcc agctgccaac tggcagatgc tgcaaactga ttgacgtatc
     7681 aaatactgtt ggctagcttc aattggagcc ttagttgcgg tacttcttac ctcgggtggg
     7741 ttgaggacca aaggtattcg gtcgcgaacg ggccgttgga gcaaacggac ccattggtat
     7801 tcgcgtccat tcttcggcag ccggaaggag cgaaccgatg tcgccggcga accaaaaaca
     7861 atcgcgtgga tttacgcttg tggagctgct ggtggtcatc gcgatcatcg gcgtgctcgt
     7921 ggcgctgctc ttgcccgcgg tgcaagcagc ccgcgaggcc gcgcggcgga tgaagtgcgt
     7981 caatcacctc aagcagttcg gcctggcgct gcacaactac gcggacgttt acggcgtctt
     8041 ccccgcccgc cgctacggaa cgacttcaac ggtcgcaagc gtctggctcc acaacaacgt
     8101 gcggatcaac gcgcacgtcg cgctcttgcc gtacatcgag caagggaaca tgttcaaccg
     8161 gatccaagcc ggagatatga cgcgcacccc tgacccgatc ggacccgggg gtccgcgcgg
     8221 cgatcagaat tgggccgtct ggaacgcacc ccccgccata cttcgatgcc cctccgatgc
     8281 cggcgcacca cggcatctcc gcaaccactc ttatgtcttc agtgcgggcg accaggtcgc
     8341 caacgtcaat accatcacgc tcagccgcgg cttgtttggc cggacgagct ggcgcaggtt
     8401 cgccgagatc agcgacggca cgagcaacac gatcgcgatg agcgagattc tgagccacgg
     8461 gccggaattc ggcggccagt ttggcctgac ttccaccgcc ggccaggtca aaatcggcct
     8521 gggactcgcc aacaacattc ctggagtcgt gaattcaccg tcggtctgcc ggaccgtttc
     8581 caacggcacg cactacgtcg cggggacaaa cgtccgcggc cgccgcggca tcaattggac
     8641 cgacgccccg gccacgctgt gcatgttcac cacggtgctg ccgcccaatt cgccgacctg
     8701 cgccgaaggc ggcgaccacg gcgaccagga caacatcgtg ttgccgccgg tcagctcgca
     8761 tcccaacggg gtcaacggcg tgatgtgcga cggctccgtc cgcttcatca ccaataacat
     8821 caacaccggc aacctgggca tcaggcagcc gcaagatggt gggagcgttt acggcgtctg
     8881 gggcgcaatg gggtcgatcg ccggcggaga agcgtccccg acggaatgag accattccca
     8941 tgaagcgctt cgcaacgctg ctggcgatcg ccgcgatttc ggccggctgc tatgggtcgg
     9001 gccggccgcc gacttatccg gtaagcggca ccgtcacctg gcaagggaag gccgtcgaag
     9061 gcgcgcgggt cgtcttcgtg ccgacgggca gccaggaatc ggccgcggga atcactgacg
     9121 ccgccggcaa gtatcagctc acgaccttcg tggccggcga cggcgcggaa ccgggcgagt
     9181 accgggtgaa agtggccaag tacgacatca agcagccgac gaaggaggag ctggagaagt
     9241 tcatcacggc cgaggaagag cagaagatcg tctttgccca ggacgaaaag cccacgccgc
     9301 cggccaagaa tctgatgcct aagaagtacg agagcgagat cacctcgggc atcacgcaca
     9361 ccgtgacgac cagccccacg acgctggata tcaaggtcga gtaaggccgc ggtgaagcgc
     9421 ggcgtcaggc ctcgatgcgg atgcacttgc catagatcgc gctggcggtg atccagccgt
     9481 tgattcgccc gcggttgttg ccgatctgga accgcggacc ttgacgggcc ttgaccaggt
     9541 gcaggtactc gcggccgttg accttgcaga gcacgatgtc gccgacctgc agcgtcgccg
     9601 gatcgaccgg ggcgaccgtg cacagctgcc cggattcaat cttgcccgcc atcgaattgc
     9661 cgcggggacg gaaagacacg gtctggcccg cccgcagctg ctcgatgtaa accgccgccc
     9721 agcccatccg gaatctccgc gcgcgcaatt gtgacgtcgg ccagacatct ggatctgccg
     9781 attggttcgc tgccgccggc cggtcgttcc agatgctagc aaaccggccg cccgcgggcg
     9841 gcatttcgca tgtccggcca gccgccaggc cgatgaatag gaatagcgca cctatttcca
     9901 ggagttttcg aatgccgagt cgttgccggc tgtggttcgc ggcctgtgtc gttgccgggc
     9961 tgctgctttc cgcttcctct gcgcaggccc agtcgagctg gtcgttttcg atcggcaccg
    10021 gcggctacgg ccatcatcac cattgccatg acggctggtg cgggccctat tgggggccgt
    10081 attacggacc gggtttcggc gtcgtctatg ccgcgccgcc gccagtggtg cgggagcgcg
    10141 tggtctatgt gcagccgcag cagccggcgt ggaccgcgcc ggccaatgtg acggccgcgc
    10201 cgacggccaa cgcgctgccc gccaacgcag cggaagacga ccggatcgtg atccggaaca
    10261 tggcgggcgc acggctgccg gtggcgttca tggtggatgg ccaggacgtc gagctgaccg
    10321 acggcgcgac gcggacgttc gtcggccgca cccgccgcat ggtgacgtac gaccgcggcg
    10381 gccgctacgg cagcaccgag cagcagctca ccgccgggca ctatgaattc cggatcacgc
    10441 ccagcggctg ggacctcgtc cgccggcccg atgtggctcc cgcgagccgc accgcggtgc
    10501 gcagcaatgc gctgcccgag cggtaatgct caggccactc gctccgcgaa cggtcagcga
    10561 ctcgcgatgc gggtcgccca caattgctgc cgccctcgag cgccagtacg attgcagccg
    10621 tcgtgatcgc cctccactcg tgcggaaggc ctctatgcgc ttgtctgcga tcgtgctgct
    10681 cgcgggaatt ctggcaggct gcgggccatc ggccacgcag aagaagctgg agcagtattt
    10741 cgtcgattgg ctgaagaacc acggcgagaa gcaagtcgtg gtcgattccg gcggcgtcgg
    10801 cctggccggc aacgccacgc ggctgaaggc ctcgatttac ggctccaagc ggcacgatgg
    10861 gggccactcg gtcgagacgg agtttcgcgt caagctgccc ggcggcggcg agatcgtcga
    10921 attcgtggcg ggcatgggcg acacgcacga ggcggccatc gacgacagcc tggtcaattt
    10981 cacgctgagc acctaccacg tcgtttacaa gtgctttctc aacgacgccg acccgcacca
    11041 gccgctgaag aagctcgcaa tcggcggcgt ggagcgcgac gtggcctttg gcgacttgtt
    11101 tgtccgcggc agcgcggggg aaaagcacaa cctgggcgag ctgcagccgg cgatcgagca
    11161 ggcgatcgcg ggactctcgc tgggtgacgg cccgcactgg atcaagatcg tctatggcca
    11221 ggccaacggc gaagtcatcg tcgtcgctgc cacgctcgac aacggcgacg cgaacgcact
    11281 gacggaaacc gtccggcaga tgaactggcc caagagcgag agtttttaca tggccaagca
    11341 gttcatcgtc atcaagtagc ggccttaagc gctatccgcg agctccggga gcaggttgct
    11401 cccgtctaag ggagccgcgc gtcaaaaccc gcacaatcgc caccggagtc aagctccgcc
    11461 ccagcgcgaa gcatggcgta gatttggccg tcgattctgc gcactggcta ccaccactca
    11521 ccagactcac ttcccaacca tggtcgtcat tgtcggctcc atcgtcg
//
LOCUS       DASRJB010000101         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_30563, whole genome shotgun sequence.
ACCESSION   DASRJB010000101 DASRJB010000000
VERSION     DASRJB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30563"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="VFB80_03075"
     CDS             <1..80
                     /locus_tag="VFB80_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZN32766.1"
                     /translation="DGKIFVSDLKDVVRIRTGEQGEDAL"
     gene            173..>2787
                     /gene="glnD"
                     /locus_tag="VFB80_03080"
     CDS             173..>2787
                     /gene="glnD"
                     /locus_tag="VFB80_03080"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZN32767.1"
                     /translation="MSRLSPAVLAARETLAAGRDKLRRQHDAGSPGVQVCARWTDLLE
                     GVVVQLFREALAELSESDRAAAEPGLAVVAHSGFGRREMAPYSDIDVMLLHARQARDR
                     GSQAVRRFSQNLYDTGMDIGFAARTPEEACSLAMSDATVLTSLVEGRQIAGDTDLYAA
                     FDGRFRRMTKRRWRRLLAAVEQARREERRKFGETVFLLEPNVKRTRGGLRDLQLIRWI
                     GFIRYGEGDVDGLAQAGRLTKEEQQQLRQARDFLLWLRNDLHFTASAATDVLDRTEQL
                     RITELRNYPQVAGLLPVEQFMREYFQHTSRVREIAGHLAEGARPVALSRWLFDGLFTH
                     RFEGEFRVGPATIAVTPKGLEQLQGDLAEVLRLLDLANRTDKRIAYPTWQAIRTSMQE
                     RGPADPNQPLPRAASERFLSLMSQPARLGELLRKLHELRVLEQLVPGMTHARGLLQFN
                     AYHRYTVDEHSIRVVEHLASLASDPGRPGEVYRGLQNKELLHLAALLHDLGKGYPQDH
                     SEVGATLAAQTASRLGLPPHDAELLQFLVLKHLRMSHLAQQHDITDDQVVVQFAVEVG
                     SPEVLKMLYVLTIADLAAVGPGVLNEWKQQLLTDLYDHTLRLLSSDSPADAASARQRQ
                     RREEVLSLARKHEGQGWWETQILSLPSSVLFSGPPAQIVEELDRIRTHSRLDAQAWGR
                     HLPARNVVEYTVGTYEEIAPGIFHRLTGALTSLRQQIFSADINTLAEGVVLDRFWVRD
                     QDFSGPPPAERLDEVCAALVSALKDHSGKPPVFRKVWGQKPTAQQSAIQHLPTRVTFD
                     NTTAERFTIVATFAYDRMGLLYAIARTLFELGLSVSKAKIGTHLDQVVDVFYVSDQHT
                     GRKIDDEVRLAEIRRR"
BASE COUNT          449 a          987 c          927 g          424 t
ORIGIN      
        1 gcgacggcaa gattttcgtc tccgacctca aggacgtcgt tcgcatccgc accggcgagc
       61 aaggcgaaga cgcgctgtag ggctagggct gggggctagg gcgcggctgg aggccgactt
      121 cagcgcccct ttgcattcac cctagcccca gccctagccc ctagcccttc ccatgtcccg
      181 cctcagcccg gccgtgctcg ccgcccgcga aacgctggcg gcggggcgcg acaagctccg
      241 ccgccagcac gatgccggct cgccgggcgt gcaggtctgc gcccgctgga ccgatttgct
      301 cgaaggcgtc gtcgtgcagc tctttcgcga ggcgctggcc gagctgagcg agagcgaccg
      361 ggcagccgcc gagccgggcc tggccgtcgt ggcccacagc ggcttcggcc gccgcgagat
      421 ggcgccttat tccgacatcg acgtaatgct gctccacgcg cggcaagccc gcgatcgcgg
      481 cagccaggcg gtgcggcggt tctcgcaaaa cctgtacgac accggcatgg acatcggctt
      541 cgccgcccgc acgccggaag aagcgtgcag cctggcgatg tccgacgcga ccgtgctgac
      601 ctcactggtg gaaggccgcc agatcgccgg cgacacggac ctgtatgccg ccttcgacgg
      661 ccgcttccgc cgcatgacga agcgccgctg gcggcggctc ctggccgcgg tcgagcaggc
      721 ccgccgcgag gagcgccgca aattcggcga aacggtcttc ctgctggagc ccaacgtcaa
      781 acggacccgc ggcggactgc gcgacttgca gctgatccgc tggatcggct tcatccgcta
      841 cggcgaaggc gacgtcgatg gactcgcgca ggccggccgc ctgaccaagg aggagcagca
      901 gcagctgcgc caggcccgcg acttcctgct ttggctgcgc aacgacctgc acttcaccgc
      961 cagcgcggcg accgacgtgc tcgaccgcac cgagcagctc cgcatcaccg agctgcggaa
     1021 ctatccgcaa gtcgccggcc tgttgcccgt cgagcagttc atgcgcgagt acttccagca
     1081 taccagccgc gtccgcgaga tcgcggggca cctggccgag ggtgcgcggc cggtggcgct
     1141 gtcgcggtgg ctgtttgacg ggctcttcac gcatcgcttc gaaggcgagt tccgggtcgg
     1201 gccggcgacg atcgccgtca cgccgaaggg cctggagcaa ttgcagggtg acctggccga
     1261 ggtgctccgc ctgctcgacc tggccaaccg cacggacaag cgcatcgcct atcccacctg
     1321 gcaggcgatc cgcacctcaa tgcaggagcg cgggcccgcc gacccgaatc agccgctgcc
     1381 ccgcgctgcc agcgagcggt tcctgtcgct catgtcgcag ccggcgaggc tgggcgaatt
     1441 gctccgcaag ttgcatgagc tgcgggtgct ggagcagctg gtgccgggca tgacgcacgc
     1501 ccgcggactc ttgcagttca acgcctacca ccgctacacc gtcgatgagc actcgatccg
     1561 cgtggtcgag cacctggcgt cgctggcatc cgatcccggc cggccgggcg aggtctatcg
     1621 cgggctgcaa aacaaggagc tgctgcacct ggcggctctg ctgcacgact tgggcaaggg
     1681 ctatccgcag gaccacagcg aggtcggggc cacgctcgcg gcccagaccg cctcgcggct
     1741 gggcctgccg ccgcacgacg ccgagctgct gcagttcctc gtgctcaaac acttgcgaat
     1801 gagccacctc gcccagcagc acgacatcac cgacgaccag gtcgtcgtgc agttcgccgt
     1861 cgaggtgggt tcgcccgaag tgctgaagat gctgtacgtg ctcacgatcg ccgatctggc
     1921 ggccgtcggc ccgggtgtgc tgaacgagtg gaaacagcag ctgctcaccg acttgtacga
     1981 ccacacgctg cggctgcttt cgagcgattc cccggccgac gcggccagcg cccggcagcg
     2041 gcagcggcgc gaggaagtgc tctccttggc ccggaagcac gaggggcaag gctggtggga
     2101 gacgcagatc ctgtcgctgc cgtcgagcgt gctcttctcc ggtccgccgg cacagatcgt
     2161 ggaggagctc gatcgtatcc gcacccactc gcggctcgac gcccaggcct ggggacggca
     2221 tctgcccgcg cggaacgtcg tcgaatacac cgtcggcacc tacgaggaaa tcgcgccggg
     2281 catcttccac cgcctgaccg gggcgctgac gagcctgcgg cagcagatct tctcggccga
     2341 catcaatacg ctggccgagg gcgtggtgct cgaccggttc tgggtccgcg accaggactt
     2401 ttccggtccg ccgccggccg agcgtctcga cgaggtctgt gcggcgctgg tcagtgcact
     2461 gaaagaccat tccggcaagc cgccggtttt tcgcaaggtc tggggccaga agcccacggc
     2521 ccagcaatcg gcgattcagc acctgccgac gcgggtcacg ttcgacaaca cgaccgccga
     2581 gcgattcacg atcgtagcga ccttcgcgta cgaccggatg ggcctgctct acgcgattgc
     2641 ccgcaccttg ttcgagctgg gcctgtcggt cagcaaggcc aagatcggca cgcacctcga
     2701 ccaggtcgtc gatgtgttct acgtcagcga ccagcacacg ggccgcaaga tcgacgacga
     2761 agtgcgcctc gccgaaatcc gccgccg
//
LOCUS       DASRJB010000102         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1157609, whole genome shotgun sequence.
ACCESSION   DASRJB010000102 DASRJB010000000
VERSION     DASRJB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157609"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFB80_03085"
     CDS             complement(<1..311)
                     /locus_tag="VFB80_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32768.1"
                     /translation="MCFFLTVAVPKKFAGRMGEAFGRGFQSCRTANPGITSALPLNFE
                     ARLILSGMCSCDLCTRPKRDSVDPVERLRQRYAQRGWSRAKTERAIAQSVAKASRRVK
                     QP"
     gene            complement(361..1032)
                     /locus_tag="VFB80_03090"
     CDS             complement(361..1032)
                     /locus_tag="VFB80_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN32769.1"
                     /translation="MDALLRAIWNDDRARASALLRADRSLATRLIDEARLYEGRIFHW
                     IYAGDTALHLAAGGYRVEIGRALLAAGADPNSAANHRRSGPLHYAADGYVSGPAWDAQ
                     RQVKTIRCLLDAGADLQAQDKNGATPLHRAVRTRCADAVECLLAAGSDPTRKNKSGST
                     PFHLAVQDTGRGGAGSDQARAGQRQIVQLFLAIGVSTGLKDGRGKTVLDCARSPAVQA
                     MLRGD"
     gene            complement(1080..2153)
                     /locus_tag="VFB80_03095"
     CDS             complement(1080..2153)
                     /locus_tag="VFB80_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN32770.1"
                     /translation="MLRGEGIIMPARNRQILLASRPTGAPRLDNFRLVESDVPRPGPG
                     QMLLRSIYLSLDPYMRGRMNAGKSYARPVEIGEVMEGRTVAEVVESNLPSYQPGDIVF
                     APCGWQEFAVSDGQGVRKLDPALRPLSYALGILGMPGMTAYTGLLNIGQPKPGETLVV
                     AAASGAVGSLVGQIGRLKGCRVIGIAGGLEKCRFVTEELGFDECLDHREPNLADKLAA
                     ACPQGIDIYFENVGGAVFDAVLPLLNNFARIPVCGLIAHYNATDLPPGPDRVPLVMHQ
                     ILVKRLTFRGFIVWDFASQLPQFLADMSAWLREGRLKYKEDITDGLENAPRELIGLLR
                     GTNFGKKLIRVSPEPRAPSAAPG"
     gene            complement(2307..>2609)
                     /locus_tag="VFB80_03100"
     CDS             complement(2307..>2609)
                     /locus_tag="VFB80_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32771.1"
                     /translation="VGGTFSAIPGGDTVPRIRFSLSTALLLVVIVAQQLAIGGLLWDR
                     RQTDLSDEAARRDADRQRLTMRAAIEDADRQQMAMRSVIEELQRRIEMLESERPIC"
BASE COUNT          454 a          856 c          846 g          453 t
ORIGIN      
        1 ggctgcttta ctcgcctaga ggccttcgcc accgattgtg cgatggcacg ctctgtcttg
       61 gcccggctcc agccgcgctg tgcataccgt tggcggagac gctcgacggg gtccacgctg
      121 tctcgtttcg gcctggtgca cagatcgcac gagcacatgc cgctgagaat aagccgcgcc
      181 tcgaagttca aagggagcgc cgatgtgatg cctgggttgg cggtccggca cgactggaag
      241 ccgcggccaa atgcttcgcc catccggccg gcaaacttct tgggcacagc gacggtcagg
      301 aagaagcaca tggatcggcc catccgccaa ggggcgacgc cgcggtcaga gctgcgtgcc
      361 tcagtcgccc cgcagcatcg cttgtaccgc agggctccgt gcgcagtcca acaccgtctt
      421 cccgcggcca tctttaaggc ccgtgctcac gccaatcgcc aggaacaact gaacgatctg
      481 ccgctgcccg gcacgggcct ggtccgagcc cgcgccgccg cggccggtgt cctgcacggc
      541 gaggtgaaac ggcgtggacc cggacttgtt cttccgtgtc ggatcgctcc cagctgcgag
      601 caggcactcg acggcgtccg cacagcgcgt gcgaacggcg cgatgcagcg gcgtagcgcc
      661 attcttgtcc tgggcctgca agtcggcccc cgcgtccagc aggcagcgga tggttttcac
      721 ttgccgctgc gcgtcccacg ccgggccgct cacgtagccg tccgccgcat agtgcaaggg
      781 accgctgcgg cgatgattcg ctgcggagtt ggggtcggcc ccggcggcca gcaaagctcg
      841 cccgatttcc acgcggtatc cgccagcggc caggtgcagc gccgtatcgc ccgcatagat
      901 ccagtgaaag atccggcctt cgtagagtcg tgcttcatcg atcaggcgag tggccaggct
      961 gcgatccgcc cggagcaggg cgctggccct cgcccggtcg tcgttccaga tggcccgcag
     1021 cagagcatcc atcgtcgttc tgcaagtggt aggagatcga gcgaaagctc tgtcggccat
     1081 caaccgggcg cagcggacgg ggcgcgcggc tcggggctga ctcggataag tttcttgccg
     1141 aagttcgtgc cgcgcagaag gccaatcaat tcgcgcggcg cgttctccag cccgtccgta
     1201 atatcctcct tgtatttaag gcggccttcc cgaagccagg cactcatatc ggcaaggaac
     1261 tgaggcagtt gcgaggcaaa gtcccaaacg atgaagcccc ggaaggtcag ccgtttgacg
     1321 agaatctggt gcatgaccag tggcacgcgg tcgggaccgg gaggcaggtc ggtggcgttg
     1381 tagtgtgcaa tgagcccgca caccggaatg cgggcgaagt tgttgagcag gggcagcacc
     1441 gcatcgaaca cggccccacc cacgttctcg aagtaaatgt cgatgccttg cgggcacgct
     1501 gccgccagct tgtcggccag attcggctcg cgatggtcca agcactcgtc gaatccgagc
     1561 tcctccgtga cgaagcggca tttctctagg ccaccggcaa tgccgatgac ccggcagccc
     1621 ttgaggcgcc cgatttgacc gacgagtgag ccgacagcgc cggaggcggc cgccacgacg
     1681 agcgtctcgc ccggcttggg ctgaccgata ttcagcagac ccgtgtaagc cgtcattccc
     1741 ggcatcccga gaattcccag ggcgtaggac agcgggcgca gcgcgggatc gagctttcgc
     1801 actccctggc catcggacac cgcgaactcc tgccagccgc agggagcgaa gacgatgtca
     1861 ccgggctggt agctgggcag attcgactcg acgacttccg cgacggttcg cccctccatg
     1921 acctcgccga tttccacggg ccgggcgtag gatttgcccg cgttcatgcg gccgcgcatg
     1981 taggggtcga gcgacaagta gatcgaccgc agcagcattt ggccggggcc cggcctggga
     2041 acatcactct ccacgaggcg aaaattgtcg agcctgggcg cgcccgtcgg ccgcgaggcc
     2101 aggaggatct gccggtttct ggcaggcatg attattccct cgccgcggag caaagcattc
     2161 cagtcgagcg agcgcgtgaa ctagcctcaa agtaccacat cctcgcattt ccagaagcga
     2221 gcggcgcaga accaggcagt ggcctgctgc tgccagcccc aaccgcgctc gtgggacttg
     2281 tcctgttggg cgctaattct ggcgacctag cagattggcc gctccgattc cagcatctcg
     2341 attcggcgct ggagttcttc gatcaccgag cgcatcgcca tttgctggcg gtcggcgtct
     2401 tcgattgccg cccgcatggt cagccgctgg cggtcggcgt cgcgcctggc cgcttcgtcg
     2461 ctcaaatcgg tctgtcggcg atcccagagc agaccgccga tggccagttg ctgggcgacg
     2521 atgacgacga gcagtagcgc ggtggacagc gaaaatcgga ttcgcggcac ggtatcgcct
     2581 cctggaattg cactgaaggt gccgccaac
//
LOCUS       DASRJB010000103         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3698677, whole genome shotgun sequence.
ACCESSION   DASRJB010000103 DASRJB010000000
VERSION     DASRJB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698677"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..250)
                     /locus_tag="VFB80_03105"
     CDS             complement(35..250)
                     /locus_tag="VFB80_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32772.1"
                     /translation="MPSQPSAAITAAASRCLAKLSLGDAPLLALAECLGELRELGWDQ
                     DSVNAVDFAVRQVLKELVHAEEGPTFA"
     gene            complement(388..1311)
                     /locus_tag="VFB80_03110"
     CDS             complement(388..1311)
                     /locus_tag="VFB80_03110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN32773.1"
                     /translation="MINILPRTYRLEWWHYLALLLAVAAAALYVTVQLRPGPAGLVAW
                     YLGSDFILWWGALFLLAAAAGITSHYRRPFFTRWRFGGALLLLLVGSTPFLYRSYPSS
                     YDDKPSAVRFRLPLDGPITVGWGGPTADVNYHVVAPDQRWAYDLLVTRDGKSHAGSGE
                     RAEDYYCYGLPVLAPADGTVHATFIDARDMPIGQLGGTPTGGNQIVLEVAPRQFVFLC
                     HLQPGSLAVKPGDKVAAGQVLARVGNSGNTSEPHLHIHLQDTEQNAFGEGIPLYFHGY
                     RSGGKLVERGLPTGGFKDGQLIGEIVEHVGE"
     gene            2181..2675
                     /locus_tag="VFB80_03115"
     CDS             2181..2675
                     /locus_tag="VFB80_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32774.1"
                     /translation="MSAESALAPAPSEAAGEELSVVAAEAATEVEAAVAVSDAAPVAA
                     EGGQAGSWPYEERQKTAERIRSSALPPAMRECLARVVLASGDAAGGQTRVPVDELVRA
                     VEEAVPDFLKAPVRRGTPAEHPAGDVFFSGDGLVTDAQAEEIAREQLAKSGLLRGQRV
                     RVAE"
     gene            2809..3588
                     /locus_tag="VFB80_03120"
     CDS             2809..3588
                     /locus_tag="VFB80_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32775.1"
                     /translation="MSEFTTGNAPGFQAATEAAENEVTWSGRAGQDLIATKKITIVST
                     AVDAGNTPTTTLRGGNLLAIVAATGKIDTYSPDANDGRQIAAGILEKAQDMLVLGVAT
                     DRFTQMLVHGLVREGELLNLDPRARQQLGRRFTFDRETSPQAGVLMHPRGVYRKSANY
                     TVTADDNGLLFLADAAVTFTLPTKANGLAFRFAQTANQNLVIAGSADIVHKNSAAASS
                     VTFSTANEKIGSHVLVECLYTAAGTLRWLVSNLGGTTATVA"
     gene            3719..4840
                     /locus_tag="VFB80_03125"
     CDS             3719..4840
                     /locus_tag="VFB80_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HZN32776.1"
                     /translation="MTLSTAQLLAPQTIRKAISQLDLPGTSLQNLFGWGLGGGNTVRQ
                     AGRNFSYDIFNVSRQVATARVPAQAMARTAPQSVKNITATFPRCAERITLLDEDLLNR
                     RQIGGDSLDRGPGGGGEVFITRQEAYLAQRFANVIEFQTAAMLRGTYVFLDDGDLLRH
                     GFAGSGTTVDFKVPAGNKGQLNMLGSTILNADWALAGTDIPAHLHAINAAMIQLTGFG
                     LAHVILTSTGWQYIVNNTKVQAQGGSSGPVFESLQRTSPGEFTAVLRGLPWLTFHVID
                     YGLEIWNGSSLVFTKLIENDHAAFLPEPSPRWVQYLEGSETVTEGPGGEKHERFGFHA
                     YAYPTHDPSGWELCAVLNGLPALYTPSAVAYGLITGGAY"
     gene            5031..5453
                     /locus_tag="VFB80_03130"
     CDS             5031..5453
                     /locus_tag="VFB80_03130"
                     /inference="COORDINATES: protein motif:HMM:NF018704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HZN32777.1"
                     /translation="MTQTYCLKADIEAIWPPADLLASVDDDTSGTLSATELGYITRAI
                     ERAANLMNARLELRYTLADLAANTWCRDANAAISAYWLATRRGLAAPAHLTQQYAAYL
                     ADLAEIANGKLKVPGALESFDQTPSVTNFAVNMNREPD"
BASE COUNT         1022 a         1899 c         1825 g          852 t
ORIGIN      
        1 agcctggtcg caacgtgcgc agcgacaaac tgtctcaggc gaaggtcggg ccttcctcgg
       61 cgtgtaccaa ttccttgagc acctggcgta ccgcaaagtc cactgcgttc accgaatcct
      121 ggtcccaccc cagttcgcgc aattctccca ggcattccgc caatgctagc agcggcgcat
      181 cgccgagcga cagcttagcc aggcagcggc ttgcggccgc ggtaattgca gcagatggtt
      241 gactcggcac ggcaggcggc tcgcaggtgg taagacgcgc tcggcacggt ggcgtacaag
      301 caaagccaat gccgaagtcc gctagcggct agtgctagtg gctagtgttg atgcgaattc
      361 gccaggcacc agccaccagc accagcacta ctcccccacg tgctcaacga tttcgccgat
      421 cagctggccg tccttgaagc cgccggtggg caggccgcgc tcgacgagct tgccgccgga
      481 gcggtagccg tgaaagtaga gcgggatgcc ttcgccgaag gcgttctgct cggtgtcctg
      541 caagtggatg tgcaggtgtg gctcgctggt gttgcccgag ttgccgacgc gggccagcac
      601 ctggccggcg gcgaccttgt cgccgggctt cacggccagc gagccgggct gcaggtggca
      661 caggaatacg aactgccgcg gggcgacttc cagcacgatc tgattgccgc cggtgggcgt
      721 gccgccgagc tggccgatgg gcatatcgcg ggcgtcaatg aacgtcgcgt gcacagtgcc
      781 atcggccggc gcgagcacgg gcaggccgta gcagtaatag tcttccgccc gctcgccgct
      841 gccggcgtgc gacttgccgt cccgcgtgac gagcaggtcg taggcccacc gctgatcggg
      901 cgcgacgacg tggtaattga cgtcggccgt cggcccgccc cagccgacgg tgatcggccc
      961 atccagcggc aggcggaagc ggacggcgct cggtttatcg tcgtacgacg aagggtagct
     1021 gcggtacaga aagggcgtcg agccgacgag gagcaatagc agggctccgc cgaagcgcca
     1081 gcgcgtgaag aacggccggc ggtagtggga cgtgattccc gcggccgcgg cgagcaggaa
     1141 gagggcgccc caccacaaga tgaagtcgct gccgagatac caggcgacga gccccgccgg
     1201 ccccggccgc aactgcaccg tgacatagag cgcggccgcc gccaccgcca gcagcaaggc
     1261 caggtagtgc caccattcga gccggtacgt gcgaggcagg atattgatca tcgagcttcc
     1321 caagagtggc tagtgctggt ggctagtgga cttggaatgg ccaccggccg tcaacctcag
     1381 ccgccgggat tgcagtcgtg ccgaaggtcc gcttgggaac taccgaccag gtgggccgca
     1441 ccaatccgga ggcgcgccag gagagaggcc aggactgcgg cccgaagggc cgacctaaca
     1501 tagcccaggg catcgccctg ggtaggcgcc gctcgaagca gcgctcagcc ccaacggggc
     1561 gccacaggca cgacgcaggc cgctcgcgcc gcatctttcg ccctttcagg gctgggaatt
     1621 tcctcattac atctacccag ggctcacggc tgcgccgcag cgccctgggc tatggcaggt
     1681 cggcccgttg ggccggcagg ctcgcacagg cgaggctgaa tcgcgggcgt ttcccatagc
     1741 cgccgtcgcc tccggctccg ggttaaacga gggcaaaggt ggccgcccgg tcggaaatcg
     1801 gtttttgggg gtcgaaaaag gggttccaga atggaccaca ctagtccatt ccgctagcag
     1861 gcccgaaaaa aacttacgtc gcggcgcggc ggcggaagtg gctgccgctc ctggggttgc
     1921 gccgcggccg attgacgcaa gtcagcatgt actgaacaaa atgtgtacaa gttgcttgcc
     1981 agcctcggat ggcgttccta aaacgggctc attcaagaca cccgtacaca tgcggccggc
     2041 tgatgccatc gtagccgaac acgccagagt tcggatggct gctcggagca ccgaattctg
     2101 gcgaattcgg ctacatgcag cgagcggccg gcccaaccgc gggtgaagcg gttctttctc
     2161 taacttcttg gaggcccaag atgagcgctg aaagtgcgct ggcgcctgcg ccgagtgagg
     2221 cggcgggcga ggaattgagc gtggtcgcgg cggaggccgc aacggaggtg gaggcggcgg
     2281 tggcggtgag cgatgccgcg ccggtggcgg ccgaaggcgg gcaagctggc agctggccct
     2341 acgaggagcg gcaaaagact gccgagcgga ttcgctcgtc ggcgctgccg ccggcgatgc
     2401 gggagtgctt ggcgcgggtc gtgctggcca gcggcgacgc agccggcggc cagacgcgcg
     2461 tgccggtgga cgagctcgtt cgcgccgtcg aagaggcggt gccggacttc ctcaaggccc
     2521 ccgttcgccg cggcacgccc gcggaacatc cggcgggcga tgtgttcttc tccggcgatg
     2581 ggctcgtcac cgacgcccag gccgaggaga tcgcccgcga gcaattggcc aagagcggtt
     2641 tgctccgcgg acagcgggtg cgcgtggcgg agtgaaggag agacggagag agggagggac
     2701 ggagggacag agtcgttttt cgctccgtct ctcatcgctc cgtctctccg tcgtgagcca
     2761 gttccagcgt tgtttttgtt cccatttcac acggcgagga aacgaactat gtcagaattc
     2821 acgaccggca atgcgcctgg atttcaggcc gcgaccgagg cggcggagaa cgaagtgacc
     2881 tggagcggcc gcgcgggcca ggacctgatc gcgaccaaaa agatcacgat cgtcagcacc
     2941 gcggtggacg ccggcaacac gcccacgacc acgctccgcg gcggcaacct gctggcgatc
     3001 gtggccgcga cgggcaagat cgacacctac tcgcccgacg ccaacgacgg ccggcagatc
     3061 gccgccggca tcctggagaa ggcccaggac atgctcgtgc tgggcgtcgc caccgaccgc
     3121 ttcacgcaga tgctcgtgca cggcctggtt cgcgagggcg agctgctgaa cctcgacccg
     3181 cgggcccggc agcagctcgg ccggcgattc acgttcgacc gcgagacctc gccgcaggcg
     3241 ggcgtgctga tgcacccgcg gggcgtctat cgcaagagcg cgaactatac ggtgacggcc
     3301 gatgacaacg gcctgctctt cctggccgac gccgccgtga ccttcacgct gccgacgaag
     3361 gccaacggcc tggcgttccg cttcgcgcag accgcgaacc agaacctggt gatcgccggc
     3421 tcggccgaca tcgtgcacaa gaacagcgcc gccgcgagca gcgtcacgtt cagcaccgcc
     3481 aatgaaaaga tcggcagcca cgtgctggtc gagtgcctct acacggccgc cggcacgctg
     3541 cggtggctgg tcagcaattt gggcggcacg acggcgaccg tggcctagcc agcagagtgg
     3601 gagaggggga gagaggggga gggggagatt gacttccttc tcccactctc ccactcaccc
     3661 actctccccc tcgaacaacc aacatttcca ttccatatcc aagaccggag aaccacgtat
     3721 gaccctcagc accgcccaac tcctcgcgcc gcagacgatc cgcaaggcga tctcgcagct
     3781 cgatctgccc ggcaccagcc tgcagaacct gttcggctgg ggcctcggcg gcggcaacac
     3841 cgtccgccag gccggccgca acttctcgta cgacatcttc aacgtctcgc ggcaggtcgc
     3901 gacggcccgc gtgccggccc aggcgatggc ccgcaccgcg ccgcagtcgg tgaagaacat
     3961 caccgccacg tttccgcgct gtgccgagcg gatcaccttg ctcgacgagg acctgctcaa
     4021 ccggcggcag atcggcggcg acagcctgga ccgcggccca ggcggtggcg gcgaggtctt
     4081 catcacccgg caggaagcct atctcgccca gcggttcgcc aacgtcatcg agttccagac
     4141 cgccgccatg ctccgcggca cgtacgtctt cctcgacgac ggcgacctct tgcggcacgg
     4201 ctttgccggc agcggcacca ccgtcgattt caaggtgccc gcgggcaaca aggggcagct
     4261 caacatgctc ggcagcacca ttctcaatgc cgactgggcg ttggccggca ccgacatccc
     4321 ggcccacctg cacgccatca acgccgcgat gatccagctc accggcttcg gattggcgca
     4381 cgtgatcctg accagcaccg gctggcagta catcgtgaac aacacgaagg tgcaggccca
     4441 gggcggctcg tccggcccgg tcttcgagtc gctccagcgg accagccccg gcgaattcac
     4501 cgccgtcctc cgcggcctgc cgtggctcac cttccacgtc atcgactacg gcctggaaat
     4561 ctggaacggc tcgtcgctgg tcttcacgaa gctgatcgag aacgaccacg cggcgttcct
     4621 ccccgagccc agcccgcggt gggtgcaata tctcgaaggc tccgaaaccg tcaccgaagg
     4681 ccccggcggc gagaagcacg aacgctttgg cttccacgcc tacgcctatc ccacgcacga
     4741 tcccagcggc tgggaactgt gcgccgtcct caacggcctc cccgccctct acacgccctc
     4801 ggccgtcgcc tacggcctga tcaccggagg ggcgtactaa caaagccgcg accattggtc
     4861 gcggtctgac tgaaacccgg tctgactgaa acaccgagga gccaggagcc agggaccagt
     4921 tgccagccgg acatgtgcga aacgccgtcg gctgcctggc ccgctccggc tcctcctttt
     4981 cgatattcaa cattcccgaa taccgcgacc actggtcgcg gctttgaaac atgacgcaaa
     5041 cctactgcct caaggccgac atcgaggcga tctggccgcc ggcggatttg ctggccagcg
     5101 tcgatgacga tacgagcggc acgctctcgg cgaccgagct gggctacatc acgcgggcca
     5161 tcgagcgggc cgcgaacctg atgaacgccc gcctggagct gcgctacacg ctggccgacc
     5221 tggccgcgaa cacctggtgc cgcgacgcca acgccgcgat ctccgcgtat tggctcgcga
     5281 ctcgccgcgg cctggccgcc ccggcgcatc tcactcagca gtacgccgcc tacctcgccg
     5341 acctggccga gatcgctaac ggcaagctca aagtccccgg cgcgctcgaa agcttcgacc
     5401 agacgcccag cgtcaccaac ttcgccgtga acatgaaccg cgagccggac taacaaagcc
     5461 gcgaccattg gtcgcggtct gacttaacca cagaggagcc aggagccagg gaccagttgc
     5521 cagtcggaca ggtgcgatat gccgtcggcc gcccggcccg ctccggctcc tcaatttttt
     5581 gaacttttga acttctaa
//
LOCUS       DASRJB010000104         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_879440, whole genome shotgun sequence.
ACCESSION   DASRJB010000104 DASRJB010000000
VERSION     DASRJB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879440"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2832)
                     /locus_tag="VFB80_03135"
     CDS             complement(<1..2832)
                     /locus_tag="VFB80_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HZN32778.1"
                     /translation="MHLGAARSRNSRLRRFDRERLRPLRFEPLEDRRLLANFAVGDNL
                     RNYRLAIAATAEYTSFFGGQAQAFAAIQSFVADLNTVFEREMAVHLNLVSNQSLVFVA
                     ADPYTNGNPAAMLNENQNFFNANVLASTYDIAHVFGTFPGGGAGLSSLGVVGQNGFKA
                     QGASTSSTPVGSDWLKLVAHEIAHQFGAPHSFNANSFEACFNNRSAPHAVEPASGSTL
                     MSYAGICGPDSLQPDPDGYFHSASFEQMEGFIRTVPGAGPSSTTPTGNSVPTVNAGPN
                     LTIPARTPFALAAAGSDANAGDTLTYTWEQLDPGSAPQGLPVSDTGFGPLFRSFPPAT
                     NPTRTFPRLTDLLNNINTAPLGEALPVSTRSLNFRATVRDGRGGVNSDDVLLNVFDTG
                     IPFVVTAPNTAVAWLGGTLQNVTWNTGNTNFAPINAANVNVRLSTDGGQTFPFLLASG
                     PNNGSMLITVPNINTTQARIKVEAAGNVFFDVSNANFTINASGTVPGIVQVESDGSTR
                     PVEGGPGDSYLIGPTTFPAGSVTVAVTADSQTVISSDGISFFPTLNLVFTAALPQTVF
                     VRAVNDAVNEGPHTSLITHAVTVSTSAAYPVGMLINNITAQVMDDELPPVVGVDFDRI
                     VSGGQPPPNWTIFDKAPTFAAETLSNLIRDDGVPTSIDVSLQYDPVGFASLSATAGAN
                     EGTKPLHNPSLAHIDGFNAADVPITITWSDLVPGGTYGVYVFGLSNDPGPAPYGQNVT
                     VTGANVVSYSQTLVDGQLRINDELGTSTRTLKSFEDLVIANAAGRIVVRVVPQIGGLP
                     YGLGGIAIREIPPPAVPGFTVTQTGGNTQVSEAGTTDTFSVVLDAPPASNVVINVFSL
                     DLTEVTVNPPLPLTFTPVNWSVPQTVTVTGVDDALLDGNQVSSVVLSIDDPLSDNAYD
                     PLPDQIVSVTTLDNDVAVSTLVQLVAGSVQ"
     gene            complement(2975..4108)
                     /gene="hemW"
                     /locus_tag="VFB80_03140"
     CDS             complement(2975..4108)
                     /gene="hemW"
                     /locus_tag="VFB80_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZN32779.1"
                     /translation="MTGDCRSAYIHVPFCRHRCGYCNFTLVAGRDDLIDSYLDALARE
                     LSWLAEPRQVETLFFGGGTPTHLPPQQLERLFEIVWKWLPMTVGGELSVEANPIDLDE
                     PKAELLNAAGVTRISLGVQSFNPRKLELLERDHRRTEIQRACQLSRRIANSVSLDLIF
                     GVPGESLDDWRSDLQAALALAPDHVSTYGLTFEKGTQFWSRLSRGELARVDEDLEREM
                     YERAIETLTQAGYEHYEVSNFARPGHRCRHNENYWLGGPYYAAGPGAARFIAGRREVN
                     HRSTTTYIRRVLAGHSPVAESEQLGPEDAARERLVFALRRLEGIDTSQFAAAAGFSAE
                     QLGGEAFKQFLHQGLLEHSGGRLRLTRRGLLVSDALWPKFLRR"
     gene            4201..4743
                     /locus_tag="VFB80_03145"
     CDS             4201..4743
                     /locus_tag="VFB80_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32780.1"
                     /translation="MQRLLPSFVFLALSLAAGWAAAQAPSPAADQPKEESSEPFDSRT
                     TGAVELKGDTKDWFEVLKDGKRAYRGAPPLLNNTVELSPGEYEIAINRTTRKIKIEKG
                     KKLVLLTGTLVVEGKKANFYAPYQGTERKVSANPRKLNTPMALLPGTYRVELNVEVNR
                     QVVLADGVNVVAGKKTVLKE"
     gene            4767..>5308
                     /locus_tag="VFB80_03150"
     CDS             4767..>5308
                     /locus_tag="VFB80_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN32781.1"
                     /translation="MTTSATATTTPLLTPQLLAQLERLELVSRKVFRGRMKGERKSKR
                     KGQSVEFADFRQYVPGDDLRFIDWNLFARLERLYLKLFLEEEDLHFYALIDASTSMEF
                     GEPSKLQYAKQLAASLGFIGLCRADRVKIESLGTSSRKPGPVLRGRASLWRMQEYLEG
                     IESGENVSLLEGVKNFCLRN"
BASE COUNT          955 a         1618 c         1829 g          906 t
ORIGIN      
        1 ctgcacgctg ccggcgacca gctgaacgag cgtggacacg gccacgtcgt tgtcgagggt
       61 cgtgacgctg acgatttgat ccggcaaggg atcgtaggcg ttgtcggaaa gcgggtcgtc
      121 gatcgacagg acgacgctgg aaacctggtt gccgtccagg agcgcgtcgt ccacgccggt
      181 cacggtcacc gtctgcggca cgctccagtt gaccggcgtg aaggtcagcg gcagaggcgg
      241 attgaccgtc acctcggtca ggtcgaggct gaagacgttg atgacgacgt tcgaggccgg
      301 cggcgcgtcg agcacgacgc tgaacgtgtc cgtcgtgccc gcttccgaaa cttgcgtgtt
      361 gccgccggtc tgggtgacgg tgaagccggg aaccgccggt ggaggaatct cgcgaatcgc
      421 gatgcccccc agcccatagg gcagaccccc gatctgcggc acgacgcgca cgacgatccg
      481 gccggcggca ttagcgatga ccagatcctc gaaggacttg agcgtccgcg tgctcgtgcc
      541 gagctcgtcg ttgatccgca gctggccatc gacgagcgtt tgcgagtacg agacgacgtt
      601 ggccccggtg accgtcacat tctggccgta gggcgccggt cccggatcgt tcgacagacc
      661 aaacacgtaa acgccgtaag tcccgccggg aaccagatcg ctccaggtga tcgtgattgg
      721 gacgtcggcg gcgttgaacc cgtcgatgtg cgccagcgac ggattgtgca gcggcttggt
      781 gccttcgttc gcgccggccg tggcgctgag cgaggcaaaa ccgacgggat cgtactgcag
      841 cgaaacgtcg atgctcgtgg gtacgccgtc gtcgcggatc agattcgaaa gcgtctcggc
      901 ggcgaaagtg ggcgctttgt cgaagatcgt ccagttgggc ggcggctggc cgccggaaac
      961 gatgcggtcg aaatcgacgc ccacgaccgg cggcagttcg tcgtccatca cctgggcggt
     1021 gatgttgttg atcagcatgc ccacgggata agcggcgctg gtgctgacgg tgacggcgtg
     1081 ggtgatcagg ctcgtgtgcg ggccctcgtt caccgcgtcg ttgaccgccc gcacgaagac
     1141 cgtctgcggc aacgccgccg tgaacacgag attcagcgtg gggaagaagc tgatcccgtc
     1201 gctgctgatg accgtctgcg aatcggccgt gacggcgacc gtcaccgagc cggccgggaa
     1261 cgtcgtgggg ccgatcaggt acgaatcgcc cgggccgcct tcgaccggcc gcgtgctgcc
     1321 atccgattcc acctgcacga tgcccggcac ggtcccgctg gcgttgatcg tgaagttcgc
     1381 attggaaacg tcgaagaaga cgttgcccgc ggcttcgacc ttgatccgcg cctgcgtggt
     1441 gttgatgttg ggaacggtga tcaacattga cccattgttg gggccgctcg ccagcaggaa
     1501 gggaaacgtc tggccgccat ccgtcgagag ccggacgttg acgttcgccg cattgatcgg
     1561 agcgaagttc gtgttgcctg tgttccaagt gacgttttgc agcgtgccgc ccagccacgc
     1621 gaccgccgtg ttcggcgcgg tgaccacgaa cggaattccg gtatcgaaga cgttcagcag
     1681 cacgtcgtca gaattgacgc cgccgcggcc gtcccgcacc gtcgcgcgga agttcaggct
     1741 gcgggtggag acgggcagcg cctcgccgag cggcgcggtg ttgatgttgt tgagcaggtc
     1801 ggtcagccgc ggaaacgtcc gggtggggtt cgtcgcggga ggaaacgaac ggaagagcgg
     1861 gccgaaaccc gtgtcgctca ccggcaggcc ctgaggagcc gagcccggat cgagctgctc
     1921 ccaggtgtaa gtcagcgtgt cgccggcgtt ggcgtcggaa ccggcggcgg cgagcgcaaa
     1981 cggcgtgcgg gcggggatcg taagattggg gccggcgttg accgtgggga cgctgttgcc
     2041 cgtcggcgtc gtgctgctgg gcccagcgcc cggcacggtg cggatgaagc cctccatctg
     2101 ctcgaagctg gcggaatgaa agtagccgtc gggatcgggc tgcaagctgt cggggccgca
     2161 gatgccggcg tagctcatca gcgtgctgcc gctggccggc tcgacggcgt gcggggccga
     2221 gcggttgttg aagcaggcct cgaagctgtt ggcgttgaag ctgtgcggcg cgccgaactg
     2281 gtgcgcgatc tcgtgggcca ccagcttgag ccaatccgaa ccgaccggag tcgagctcgt
     2341 cgatgcgccc tgggccttga agccgttctg gccgacgacg ccgagcgacg acagccccgc
     2401 tccgccgccg gggaaagtgc cgaacacgtg agcgatgtcg taggtgctgg cgaggacgtt
     2461 ggcattgaag aagttctgat tctcgttcag catcgcggca ggattgccgt tggtgtacgg
     2521 gtccgccgcg acgaaaacca gactctggtt cgacaccagg ttgaggtgga cggccatttc
     2581 ccgttcgaac acggtattca ggtccgcgac gaacgactga atcgcggcaa acgcctgcgc
     2641 ctggccccca aagaaactcg tgtattccgc cgtggcggcg atcgccaggc ggtagttgcg
     2701 aaggttgtcg cccaccgcga agttggccag gagccgccgg tcctccagcg gctcaaaccg
     2761 cagcggtcgc agccgctccc gatcgaaacg gcgcagccgc gaattcctgc tccgggcagc
     2821 tcccaaatgc atgcgccgat cccttacgcg tcaaagacac tgagtgaggg gctttcgtga
     2881 attccgattt cacagtgaac tagacggccg atcgattttc aagtgatttt tccgcgaaac
     2941 caacgcctgg cccggcgcgg ggcggcggca ggcttcatcg ccgcagaaat ttcggccaga
     3001 gcgcgtcgct gaccagcaag ccgcggcgag tcagtcgcaa tcggccgccg ctgtgctcca
     3061 gcaacccctg atggagaaac tgcttaaatg cctcgccgcc gagttgctcg gcgctgaagc
     3121 cggcagcggc tgcgaattgg ctcgtgtcga tgccttccag acgccgcagg gcaaagacca
     3181 gccgttcgcg ggcggcgtct tcggggccga gctgctcgga ctcggcgacc ggcgaatggc
     3241 cggctaggac gcggcggatg taggtcgtcg tgctgcggtg gttgacttcc cgccggcccg
     3301 caatgaaccg cgccgcgccg gggccggcgg catagtatgg cccgccgagc cagtagtttt
     3361 cgttgtgccg gcagcggtgg ccgggccggg caaaattgga gacttcatag tgctcgtagc
     3421 cggcctgggt aagcgtctcg attgcccgct cgtacatttc ccgctccagg tcctcgtcca
     3481 cgcgggccaa ttcgccgcgc gacagccgag accagaactg cgttcccttt tcaaacgtca
     3541 ggccgtaggt ggagacgtgg tcgggcgcga gggcaagcgc cgcctgcagg tcgctgcgcc
     3601 aatcgtcgag tgactcgccc ggcacaccga agatcaggtc gagcgagacg gaattcgcaa
     3661 tgcggcgcga tagttggcag gcccgctgga tttcagtccg ccggtgatcg cgctccagca
     3721 gctccagctt ccgcgggtta aacgactgca cgcccagact gatgcgcgtc acgcccgccg
     3781 cgttcagcag ctctgccttt ggctcgtcca gatcgatcgg attggcctcg acactcagct
     3841 cgccgccgac cgtcatcggc agccacttcc acacgatctc gaacaagcgc tccagctgtt
     3901 gcggcggcag gtgcgtcggc gtgccgccgc cgaagaacag cgtctcgact tgccgcggct
     3961 cggcaagcca ggagagctcc cgcgcgagtg cgtcgaggta cgagtcgatc aggtcgtcgc
     4021 ggccggcgac aagcgtgaag ttgcaatacc cgcagcgatg ccgacagaac ggaacgtgga
     4081 tgtaggccga gcggcagtcg ccggtcatgg ttctcgcagc cgtttgcacc gagcttcctg
     4141 ggccattcaa cacgaattga tagtatgatg gcgtttccat gatcggcgaa ggagattgcg
     4201 atgcagaggt tgcttccatc gttcgtattt ctggcgctgt cgctggctgc cggctgggcc
     4261 gcggcccaag cgccgagccc ggccgccgat cagccgaaag aagagtcgag cgagccattc
     4321 gacagccgga cgaccggcgc cgtggagctc aagggggaca ccaaggactg gttcgaagtg
     4381 ctcaaggacg gcaagcgggc ctaccgcggc gcaccgccgc tgctcaataa cacggtggaa
     4441 ctgtcgccgg gcgagtacga gatcgcgatc aacaggacga cccgcaagat caagatcgag
     4501 aagggaaaga agctggtgct gctcaccggc acgctcgtcg tcgaggggaa aaaggccaat
     4561 ttctatgctc cctaccaagg aacggagcgc aaggtcagcg ccaatccgcg gaagctgaac
     4621 acgcccatgg cgcttttgcc ggggacgtat cgcgtggagc tcaacgtcga ggtgaaccgg
     4681 caggtcgtcc tggccgacgg tgtgaatgtt gtcgcgggga agaaaactgt tttgaaagaa
     4741 tagccgccaa ccggcgaatt acgacgatga caactagtgc gacggcaact acgacccccc
     4801 tgctcacgcc tcaacttctg gcccagctcg agcggctgga gctcgtcagc cgcaaggtgt
     4861 ttcgcgggcg gatgaagggc gagcggaaga gcaagcggaa ggggcagagc gtcgagttcg
     4921 ccgacttccg gcagtacgtg cccggcgacg acttgcggtt tatcgactgg aatctcttcg
     4981 cccggctgga gcggctgtac ctgaagctct ttctggaaga agaggatctg cacttctacg
     5041 ccctgatcga cgccagcacg agcatggaat tcggcgagcc ctcgaagctg caatacgcca
     5101 agcagctggc ggcttcgttg ggcttcatcg gcctgtgccg ggcggaccgc gtgaagatcg
     5161 aatcgctggg caccagcagc cgcaagccgg ggccggtgct ccgcggccgc gcgagcctgt
     5221 ggcggatgca ggagtacctg gaggggatcg agtcgggcga gaacgtgtcg ctgctggaag
     5281 gggtgaagaa cttctgcctc cgcaacag
//
LOCUS       DASRJB010000105         8636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2570427, whole genome shotgun sequence.
ACCESSION   DASRJB010000105 DASRJB010000000
VERSION     DASRJB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570427"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1441
                     /locus_tag="VFB80_03155"
     CDS             59..1441
                     /locus_tag="VFB80_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32782.1"
                     /translation="MSDAFSLARTCGDFRRQPDVSRRAFVRAGALGAAGLSLPALLRS
                     EARAAAAHKQQNNVILLWMRGGPSHIDMWDPKPEAPAEFRGEFGTMNTSVTGIQLTDM
                     LPKCAAVMDRWSIVRSLHHGDAGHSTGDQICFTGYGPGINPDQNIHPSCGSIVARQLS
                     RLNPELPAYVMIPRQVPGTGSAYLGVACNPFETGADPANNGPFQVQNFQLAGGLSVDR
                     LGSRRDLLGSLDRLRAGVDASGRMEGLDQFGQKAWEMLSSPAAQKAFDLGSEPQSMRE
                     RYGLMPAFDPGAANRCGAPNWSQRMLLARRLVEAGVRLVTVDLRWWDTHVQGFDSLRR
                     GFLPRFDQAYAALISDLHERGLLSRTLVVAWGEFGRTPRVNNDAGRDHYPNVFSAALA
                     GGKVKGGRVVGSSDSKGAFPKDNPKSPQDVLATIYDYLGVDVGQHYQNESGRPIRVLP
                     SGEPIRELFS"
     gene            complement(1524..2885)
                     /locus_tag="VFB80_03160"
     CDS             complement(1524..2885)
                     /locus_tag="VFB80_03160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZN32783.1"
                     /translation="MDEILAYLEKNAGRFEDQLSELLRIPSISADPARGDDVRRAAAW
                     VEEQFRNLGLLTEIVQTSGQPLVVAQSPPVPGKPVVLVYGHYDVQPVEPLEEWKSPPF
                     EPTKRSGNIYARGATDDKGQMLTHVLSTTAWLKTRGKLPVQLKFLIEGEEEVGSKGLY
                     EYLATPAAKDKLKCDVVVISDTSQYGPGQPAITYGLRGIAFFELKVTGPKQDLHSGTF
                     GGSVTNPINAICQLLASLIDEKGRVQVPGFYDAVAPLSDRERQQFAALPFTDQAYMEQ
                     LGVKALAGETGYSTLERRWVRPTFDVHGIWGGQQVSKTAIPARAGAKFSFRMVPDQDP
                     DKIAAATREFLYARKPPGVEVELITSHGAKAVVVPLDSPYVAAASRAIESGFGKKPVF
                     IREGGSIPIVTTFKERLGIDTLLLGWGLDDDNTHSPNEKFNLGDFHRGIKSSAYLWQE
                     LAS"
     gene            3010..3378
                     /locus_tag="VFB80_03165"
     CDS             3010..3378
                     /locus_tag="VFB80_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HZN32784.1"
                     /translation="MSSEKSAVAEPEVADPVPAQKKKPKPKRKPPRKMPMYHVILWND
                     NDHSFDYVIEMMRKLFGHTRERGYLIAKEVDSSGKAICVTTTLEHAELKRDQIHAFGP
                     DKLIERCKGSMSATIEAAPQ"
     gene            3406..3903
                     /locus_tag="VFB80_03170"
     CDS             3406..3903
                     /locus_tag="VFB80_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32785.1"
                     /translation="MRIAGIVLLVAGALMIATSVLKLAVGNRGTDGDGLSESQLRMDA
                     VVPGAIVGVIGFALVVLSRPKPVVTAVAAEDWAPVKLSEAAADFAKRAMVERKFAPET
                     ALRIMEDSESGSWSVKFDLQSDGDEDVVGEDQGVIVVVEKSLAPRVAGTLIDIEGGTF
                     LVRLS"
     gene            4027..6885
                     /locus_tag="VFB80_03175"
     CDS             4027..6885
                     /locus_tag="VFB80_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN32786.1"
                     /translation="MSRRFSFRPCRIIRATRQTLLHLERLEERTFLAGDINLVGNGNA
                     IVNGDTTPRTADNTDFGSVTAFGAVSVSKSFTVQNTGADPLNLTGAPTIAISGSAAAD
                     FTVTAFPSTPVAGAGSTTFTVTFDPSTTGTRAATITILSDDPDESPYSFAIRGAGSGP
                     DIVVLGGTTLIADGDSTPSTADLTDFGGTALEGGTIVRTFTIGNAIPFTTLNLTGSPD
                     RVTISGANASDFVVSVQPAASVTSPLSPTSFQITFNPSGPGLRTAVVSIANNDPDENP
                     FDFAIQGIGGFPDLDVAVGEVSIADGDVTSLPGDVTDFGIADLSGDSFTKTFTIRNTG
                     DNFLNLGSPPAVTITPFSPLPGVELPGAGDFTVILLPELSTIGPGGSTTFEITYNPSP
                     LPAGGINTSPLNPDLSSPNYNSVGSTDLFRFATVNIASDDTDENPYNFVVSGTAIVGT
                     VEATLAGGVLTVSDIHKTGRNNGFVLSVVGEDLIVSDALSLETFTVASAANTGGTLSL
                     VNKVLTIPLAGITTVIVNGNGGSDTFNLGLLLGLTAVTLNGGDGDDTFGSNTLGNGLD
                     NIIPFPTTRITINGGGASPAPGGDRLFLDLSTVPSLPAIWLGTAPTTQITTLRALPYQ
                     PFAYSQVEIVALRDGNEPTNFAAGDFYARGGDLIPDRLEMRPNATVNTAKFYYTGLNI
                     GTFLVPGKVLFYGRGGGDTLVSTYNGHAAELYGEDGNDWLSGANAADKAVGGAGNDNI
                     NAGEGHNVVWGDKDPVEVGLSDTDANRELLANDVAGAASAFHATGTFVDTIRTGGGND
                     VIYCGPGNDGSLGLITSGGGNDYVHGGEGNDQLDLGAGHDRGYGGPGNDTLYGRDGND
                     FLGGGDGNDICFGGNGHDIISGGAGNDLLFGDAGTDRVYQGSLTVTVTTPPLVGTDQS
                     KRKDDLADQAMLALLNDWADNQILGNVSVATDAALSVG"
     gene            complement(6892..8418)
                     /locus_tag="VFB80_03180"
     CDS             complement(6892..8418)
                     /locus_tag="VFB80_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32787.1"
                     /translation="MNERILVRGELSVWVHVGRALGALAFFLGLILGATAGPWGWAIA
                     VGGLFIWIILEVVAFQARRVRTWLTLHPDGIEVESPAGHRAIHDSQVSAVALETKKNL
                     SNGELASISRKFTLWAADQPEPVLMENKIKVNAPDPLADLINRLLDRLRGRFEQDLAK
                     GGTVSGDNWHLSRTALTLGRPPHDQQIPVSEITAIETSGGEMCVWRKGSDVAAAKLPL
                     SSRNVYLLPSLVQPFLPEEKADLGGADSASGLGRILFEKRPNRPTVMTVAVAGLIMLA
                     VGIVMLAAGVRPGGNNEAPFIAALVMLVLGPILTLTGTAMAYMNFRCHERGVWQSNLL
                     GQKTLRYTDVGSFQYGATRHYHNGVYTGTHLSLQFRPISADRGPTIRYGTTTRGDDDD
                     LDNLRDFISRAIAARMAEQFNAKQPVPWTANLEFLPEGIRYKPDGFLGRKAAQLLPYS
                     DYGGYDMQQGVFYLFARENKKYVATEQASADNFFPGFFLLLLLLHSSAEEEPAEAAEA
                     "
BASE COUNT         1509 a         2864 c         2773 g         1490 t
ORIGIN      
        1 cgccgatata taaattgtgg tgcatgctta gcaccatgac ttgggggcgg ctcgacaaat
       61 gagcgatgcg tttagcctgg cgcggacttg cggtgatttt cgccggcagc cggatgttag
      121 ccgtcgggct ttcgtgcggg ccggagcgct gggcgcggcg ggattgtcgc tgccggcgct
      181 cttgcggagc gaagctcggg ccgcggcggc ccacaagcag cagaacaacg tgatcctgct
      241 ctggatgcgc ggtggcccga gccacatcga catgtgggat cccaagcccg aagcgccggc
      301 cgagtttcgc ggcgaattcg ggacgatgaa caccagcgtc acgggcattc agctcaccga
      361 catgctgccc aagtgcgccg cggtgatgga ccgctggtcg atcgtccgca gcctccatca
      421 cggcgacgcc ggccactcga ccggcgacca gatttgcttc accggctatg gccccggcat
      481 caatcccgac cagaacatcc atcccagctg cggctcgatc gtggcccggc agctcagccg
      541 cctgaatccg gagttgccgg cctatgtgat gattccccgg caggtgccgg gcacgggctc
      601 ggcctatctg ggcgtggcct gcaatccgtt cgaaaccggg gcggacccgg cgaacaatgg
      661 cccgtttcag gtgcagaact ttcagctggc cggcggcttg tcggtcgatc ggctcggctc
      721 acggcgcgac ctgctgggca gcctcgatcg gctgcgggcc ggcgtcgatg ccagcggccg
      781 catggagggg ctcgatcaat tcggccagaa ggcctgggag atgctcagct cgcccgccgc
      841 ccagaaggcg ttcgacttgg gcagcgagcc gcaatcgatg cgcgagcggt acggcctgat
      901 gccggcgttc gaccccggcg cggccaaccg ctgcggcgcg ccgaactgga gccagcggat
      961 gctgctggcc aggcggctgg tcgaggccgg cgtgcggctg gtcacggtcg atctgcggtg
     1021 gtgggatacg cacgtgcagg gcttcgattc attgcggcgc gggtttctgc cgcggttcga
     1081 ccaggcgtat gcggcgctga tttccgatct gcacgagcgg ggcctgctct cgcggacgct
     1141 ggtggtggcc tggggcgaat ttggccgcac gccgcgggtg aacaacgacg ccgggcgcga
     1201 ccactatccc aacgtgttca gcgctgcgtt ggctgggggc aaagtcaagg gcggtcgcgt
     1261 cgtcggctcg tccgacagca aaggcgcatt tcccaaggac aatccgaagt caccgcagga
     1321 cgtgctggcg acgatctacg actacttggg cgtcgacgtt ggtcagcact accagaacga
     1381 atcgggccgg ccgattcggg tgctgcccag cggcgaaccg atccgcgagc tgttctcgta
     1441 gagcaagcgt cctcgcttgc tcacgtctct atcctggccg ggtgccaccg tgaatcagcg
     1501 ctgcacaagc gaggacgctt atgctacgag gccagctctt gccaaaggta cgcgctggat
     1561 ttgatcccgc ggtggaagtc gcccaagttg aatttctcgt tggggctgtg cgtgttgtcg
     1621 tcgtccaggc cccagcccag cagcagcgta tcgatgccca gccgctcctt gaacgtggtg
     1681 acaatcggta tcgagccgcc ctcgcggata aacaccggct ttttgccgaa gccgctctcg
     1741 atcgcccgcg aggcggccgc gacgtacggg ctgtcgagcg gcacgacgac ggccttcgcg
     1801 ccgtgcgagg taatcaactc cacttcgaca cccggcggct tgcgagcgta gagaaactcg
     1861 cgggtggccg cggcgatttt gtcggggtcc tggtcgggca ccatgcggaa gctgaacttc
     1921 gcgccggccc gggcgggaat cgcggtcttg ctgacctgct ggccgcccca gatgccgtga
     1981 acgtcgaagg tcggccgcac ccagcggcgc tccagcgtgc tgtaccccgt ttcgccggcc
     2041 agcgccttta cgcccagctg ctccatgtag gcctgatcgg tgaagggcag ggccgcgaac
     2101 tgctgccgct cgcggtccga gagcggcgcc acggcgtcgt agaagccggg cacttggacg
     2161 cggcccttct cgtcgatcag cgaggcgagc aactggcaaa tcgcgttgat cggattggtc
     2221 acggagccgc cgaacgtacc gctgtgcagg tcctgcttgg ggccggtcac cttcagttcg
     2281 aagaacgcga tcccccgcag accgtaggtg atcgccggct gccccgggcc gtattgcgag
     2341 gtgtcgctga tgacgaccac gtcgcacttg agcttgtcct tggccgccgg cgtcgccagg
     2401 tattcgtaca accccttgct gccgacttct tcctccccct cgatcaggaa cttcagctgc
     2461 acgggcagct tgccgcgcgt tttcagccag gcggtggtgc tgagcacgtg cgtcagcatc
     2521 tgccccttgt cgtcggtcgc gccgcgggca tagatgttgc cgctgcgctt ggtcggctcg
     2581 aacggcgggc tcttccactc ctccagcggc tccactggct gcacgtcgta gtgcccatag
     2641 accagcacta ccggcttgcc aggcacgggc ggcgactggg cgacgaccag cggctgtccc
     2701 gaggtctgga cgatctcggt gagcaggccc agattgcgga actgctcctc gacccaggcc
     2761 gccgcgcggc ggacgtcgtc gccccgcgct gggtcggcgc tgatgctggg aatccggagc
     2821 agctccgaga gctggtcctc aaaccgcccg gcgttctttt ccaggtaggc gagaatctcg
     2881 tccatcgaat ccgtccctgc gcgagtgaaa gcggcacgtg gccggcactt tcctccgcca
     2941 atataatgcg gcaggtgcgc cgccgcctat cgtgggccgc ggcgccgcga cgtgcgccag
     3001 gagccccccg tgagcagcga aaagtccgcc gtggccgaac ccgaagtggc cgaccccgtg
     3061 ccggcccaga agaaaaagcc caaaccaaaa cgcaagccgc cgcggaagat gccgatgtat
     3121 cacgtcatcc tgtggaacga caacgaccat tcgttcgact acgtgatcga gatgatgcgg
     3181 aagctgtttg gccatacgcg cgagcggggc tacctgatcg ccaaggaggt cgattcgagc
     3241 ggcaaggcga tctgcgtcac gacgacgctg gagcacgccg agctcaagcg cgaccagatc
     3301 cacgccttcg gccccgacaa gctgatcgag cgctgcaaag gctcgatgag cgcgacgatc
     3361 gaagccgcgc cgcagtagtc ctgccgggag ttgtgaggcg aatccatgcg aatcgcgggc
     3421 attgtactcc tcgtggcggg cgcgctgatg attgcgacca gtgtgttgaa actggccgta
     3481 ggcaatcgcg gtacggacgg agatggattg tccgaatcgc agttgcggat ggacgcggtc
     3541 gttccgggcg ctatcgtcgg cgtcattggc tttgccctgg tggtcctttc gcggccaaag
     3601 ccggttgtca cggccgtggc ggcggaagat tgggcaccag tcaagctctc ggaggccgcc
     3661 gccgacttcg cgaaacgtgc aatggtggaa cgcaaattcg cgcctgaaac agccctccgc
     3721 atcatggaag actccgaatc cggttcgtgg agcgtcaaat tcgatttgca gtcggatggc
     3781 gacgaggatg tcgtggggga agaccaaggc gtgatcgtcg tcgtggaaaa atcgctcgcc
     3841 ccgcgtgtcg ccggcacgct tattgatatt gaaggcggta cattcttggt ccgcctttcg
     3901 tgagtcgcta catccaccgg tagcccggtc ttgcgactgg cagagcggaa cgattacgct
     3961 ctctgcctga catctccact cttcgcagct tgacttcgca cccagtcgca gttggagtgc
     4021 atccctatgt cgcgccggtt ctcgtttcgc ccttgccgga tcattcgagc cactcgccaa
     4081 acgttgctgc acctggagcg cctcgaagag cgcaccttcc tggccggcga catcaacctg
     4141 gtcggcaatg gcaacgctat tgtcaacgga gacaccacgc cacgaaccgc ggataacacc
     4201 gacttcggct cggtgacagc gttcggcgcg gtcagcgttt ccaagagctt caccgtccag
     4261 aacaccggcg ccgatccgct caacctcacg ggcgctccca cgattgcgat cagcggctcg
     4321 gcggctgccg attttaccgt caccgcattt ccttccacgc ccgtggctgg cgccggcagt
     4381 acaacattta cggtcacgtt cgaccccagc acgaccggca cgcgggcggc gacaatcacg
     4441 attctcagcg atgacccgga cgaaagcccc tacagctttg cgattcgcgg tgccggcagc
     4501 ggtccggaca tcgtcgtgtt gggaggaacg acgctcatcg ccgacggcga ttccacgcct
     4561 tcgactgccg acctgaccga ttttggcgga accgcattgg aaggcggcac gattgtccgc
     4621 acgtttacga tcggcaatgc gattcccttt acgacgctca atctcacggg ctctcccgac
     4681 cgggtgacca tcagtggggc caacgccagc gactttgtag tgagcgtgca gcccgcggcc
     4741 agcgtcacca gcccgctttc cccgaccagc tttcaaatca ccttcaaccc cagcgggccg
     4801 ggcctgcgaa cggcagtcgt cagcattgcg aacaatgatc ccgacgaaaa cccgttcgac
     4861 tttgcgatcc agggtatcgg cggatttccc gatctcgacg ttgccgtggg tgaagtgtcg
     4921 atcgccgatg gcgacgtgac gtcgctgccc ggcgacgtca ccgacttcgg catcgccgac
     4981 ctgtcgggcg attcgttcac caagacgttc acgatccgca atacgggcga caactttctg
     5041 aacctgggca gcccgccggc cgtgacgatc actcccttct cgccgctgcc gggcgtcgag
     5101 ctgcccgggg ccggcgactt tacggtcatt ctgctgcccg agctttcgac catcggcccg
     5161 ggcggcagca cgacctttga aatcacctac aaccccagcc cgctgcctgc cggcggaatc
     5221 aacacgagtc cgctcaatcc cgacctcagc tcgccaaact acaactccgt gggctcgacc
     5281 gacctgttcc gctttgccac cgtcaacatc gccagcgacg acaccgacga gaacccctac
     5341 aacttcgtcg tttcgggcac ggcgatcgtc ggtacggtcg aggccacgct ggccggcggc
     5401 gtgctgaccg tctccgacat ccacaaaacc ggccgcaaca acggtttcgt gctgagcgtt
     5461 gtcggcgaag acctgatcgt cagcgacgcc ttgagcctcg agacattcac ggtggcctcg
     5521 gcggccaaca ccggtggcac gctgtcgctg gtcaacaagg tgctcacgat tccgctggcc
     5581 ggcattacaa ctgtcatcgt caacggcaat ggcggcagcg acacgttcaa tctcggcctg
     5641 ctgctcggcc tgaccgcggt cactctcaac ggcggcgacg gcgacgacac gttcggcagc
     5701 aatacgctgg gcaacggtct ggacaacatc atcccgtttc ccacgacgcg gatcaccatc
     5761 aacgggggcg gcgcctcgcc ggctcccggc ggcgatcggc tgttcctcga tctgtccacc
     5821 gtgccctcgc tgcccgcgat ctggctgggc acggcgccca cgacgcagat cacgacgctc
     5881 cgggccctgc cctatcagcc cttcgcgtac tcgcaggtcg aaatcgtggc cctccgcgac
     5941 ggcaatgagc cgacgaattt cgcggccggc gacttctacg cccgcggcgg cgacttgatc
     6001 ccggacaggc tcgaaatgag gccgaacgcg acggtcaaca cggccaagtt ttactacacg
     6061 gggctcaata tcggcacgtt cctggttccc gggaaggtgc tcttctacgg ccgcggcggc
     6121 ggcgacacgt tggtcagcac ctacaacggc cacgcggccg agctgtatgg cgaggacggg
     6181 aacgactggc tctccggcgc caacgcggcc gacaaggccg tgggcggcgc tggcaacgac
     6241 aacatcaacg ccggcgaggg gcacaacgtc gtctggggcg acaaagaccc cgtcgaggtc
     6301 ggcctgagcg ataccgacgc caaccgcgag ttgctcgcca acgacgtggc gggagcggcg
     6361 tcggcctttc atgccaccgg cacgtttgtc gatacgatca ggaccggcgg cggcaacgac
     6421 gtgatctact gtggacctgg caacgacggc tccctgggcc tgatcacctc cggcggcggc
     6481 aacgactatg tccatggcgg agagggcaac gaccagctcg acctgggcgc gggccacgac
     6541 cgcggctacg ggggcccggg aaacgacacg ctctacggcc gcgatggcaa tgacttcctc
     6601 ggcggcggcg acggcaacga catctgcttt ggcggcaacg ggcacgacat catttccggc
     6661 ggagccggaa acgacctgct ctttggcgac gctggcaccg accgcgtcta tcagggcagc
     6721 ctgaccgtca cggtgacgac ccctccgcta gtcggcacgg accagagcaa acgcaaggac
     6781 gacctggccg atcaggccat gctcgccctg ctcaacgact gggccgacaa ccagattctc
     6841 ggcaacgtct cggtcgcgac cgacgcagcg ctttcggtcg ggtagccgct attatgcttc
     6901 cgccgcttcg gccggctctt cttccgccga gctatgcaag agcaatagca gcaggaagaa
     6961 gcccggaaag aaattgtcgg ccgaggcctg ctcggtggcg acgtacttct tgttctcgcg
     7021 cgcgaacaga tagaagacgc cttgctgcat gtcgtagccg ccgtagtcgg aatagggcag
     7081 cagctgcgcc gccttgcggc ccaggaagcc atcgggcttg tagcggatgc cttcgggcag
     7141 gaactccagg ttggcggtcc agggcacggg ctgcttggcg ttgaattgct cggccatccg
     7201 ggcagcgatt gcccggctga tgaagtcgcg gagattgtcg aggtcgtcgt cgtcgccgcg
     7261 ggtggttgtg ccgtaacgga tggtcggccc gcggtcggct gaaatcgggc ggaattgcag
     7321 cgagaggtgc gtgcccgtat agacgccgtt gtggtagtgc cgcgtcgcgc cgtactgaaa
     7381 gctgcccacg tcggtatagc gcagcgtctt ttgacccagc agattgcttt gccagacacc
     7441 ccgctcgtgg cagcggaaat tcatgtaggc catggccgtg ccagtgagcg tcaggattgg
     7501 gcccagcacg agcatcacca gcgccgcgat aaagggcgcc tcgttgtttc cgcccggcct
     7561 caccccggcg gcgagcatca cgattcctac ggccagcatg atcaagccgg ccacggccac
     7621 cgtcatgact gtcgggcggt tcggccgctt ctcgaagagg atccgcccca ggcccgacgc
     7681 cgaatcggcc ccgcccagat cggccttctc ctccggcaaa aacggctgca ccaggctcgg
     7741 cagcagatag acattgcgcg acgaaagcgg cagtttggcg gccgcgacat cgctcccctt
     7801 ccgccacacg cacatctcgc cgccggaggt ttcgatcgcg gtgatctcgc tcaccggaat
     7861 ctgctggtcg tgcggcggcc ggccgagagt cagggccgtg cggctgaggt gccaattgtc
     7921 gccgctgacg gtgccccctt tggcgaggtc ctgctcgaag cggccgcgca aacggtccag
     7981 cagccgattg atcaagtcgg ccagcggatc gggggcattg accttgatct tgttctccat
     8041 cagcaccggc tcgggctgat cggcagccca cagggtgaac ttgcgcgaga tcgacgcgag
     8101 ttcgccgttg ctcaagttct tcttggtctc cagggcgacg gccgaaacct gcgaatcgtg
     8161 gatcgcgcgg tggccggcgg ggctctcgac ttcgatgccg tcgggatgca gcgtcagcca
     8221 ggtccgcacg cggcgagcct gaaaggcgac gacctccaag atgatccaga tgaacaagcc
     8281 gccgacggcg atcgcccagc cccagggccc cgccgtcgcc ccgagaatca gacccaggaa
     8341 gaaggccagc gcgccgagcg cccggccgac gtgcacccac accgaaagct cgccccggac
     8401 cagaatccgc tcgttcatag aacagcctcc gctggccaat atcctgaccg gcgaaatagc
     8461 aactggcaat cctgccaggc ctgcggccag ctgccgatgg cctaacgcgg cgcggcctcg
     8521 gcggcgacgg tcgcagcggc ctcccgcggc tccagcaggg ccaggagcgg cgaggtggcg
     8581 atcgtcgtga cgagggccat gaccaccatc atcgcgaaca gcgccggcga gatgac
//
LOCUS       DASRJB010000106         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_32147, whole genome shotgun sequence.
ACCESSION   DASRJB010000106 DASRJB010000000
VERSION     DASRJB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32147"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..530
                     /locus_tag="VFB80_03185"
     CDS             48..530
                     /locus_tag="VFB80_03185"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN32788.1"
                     /translation="MNALPSPTTFMQEAIRLAIDGAQQGRGGPFGAVVVREGQIVGRG
                     CNCVTSTNDPTAHAEVIAIRDACRQLGVFHLAGCELYASCEPCPMCLAAIYWAHLDRF
                     YYGSTAAEAAAAGFADDFIRRELTRDPEQRAIPSARLLAAEAAAAFAAWQAKADRIAY
                     "
     gene            757..1386
                     /locus_tag="VFB80_03190"
     CDS             757..1386
                     /locus_tag="VFB80_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32789.1"
                     /translation="MLQVRGGDAGAFEELVLRYQHRLLTVLEHLVGNREQAEDLTQEV
                     FLRVFRARDRYEPGAKFSTWLFTIANNVASNANRSRSRRREVGVPEGSNGSESAPLQL
                     DQLAKAASSFMPTRALDKAEQAQMVRHAVAALSERQRLALLLAKFEGMSYQDIAETMG
                     LSVQAIKSLLSRARVNLKEILTPYVEQGTPPDENKPPPQGGSKPPADDE"
     gene            1432..1941
                     /locus_tag="VFB80_03195"
     CDS             1432..1941
                     /locus_tag="VFB80_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32790.1"
                     /translation="MSDENPSLAEASTIDEELVAYLDGELNTDQISRIERRLLDDPQY
                     RARLNQLERTWDLLDTLNRSQADDDFAHSTVEMVAVQAVEEAKTETLRTIRRRNLGWV
                     AIVAAVLALTGVTYGVIQNRLAQPNRELVRNLPLIERVDDYRNIDSLEFVKELEKANL
                     FAAEVDSGD"
     gene            2069..3070
                     /locus_tag="VFB80_03200"
     CDS             2069..3070
                     /locus_tag="VFB80_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32791.1"
                     /translation="MAFGLPAAAVADETEAERAARINQMSTEDKALLAGKKKRFDELK
                     PPELAHLQGLYEAISSDAEAQKHEETIRRYNKWLADLSSGQRGKILDIKDPKERIDKI
                     KELMRKQEEERFQQFVELDVTNADRDTVYAWLEAFIVQHEDQIMTRLREEARRRIQEA
                     PDEAARRKALIDRWQSARWYVPDMPFPTKDDFNQLFAKLSDEAKTRITAGANTDEQEQ
                     RLTDLMRAAIVSRVFSQPSREDLQKFYANMKPDDPRRERLEGLDREKLYQQLRAMYYR
                     ERFPAGFRGRGGPGSRGGGPPFAPPGEGGGQRPPPKGERPPAEGNASGAPPPAGKQP"
     gene            3102..4622
                     /locus_tag="VFB80_03205"
     CDS             3102..4622
                     /locus_tag="VFB80_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN32792.1"
                     /translation="MGDTGRLLVRFFLSVLLLAGAASAAERPNIVLIYADDLGYGDVG
                     CYGAEWVKTPHIDRLAREGLRFTDGHCASATCTPSRYALLTGQYPWRKAGTGILPGDA
                     AAIISPGAPTIASTLKAAGYKTGVVGKWHLGLGSGNLDWNGEIKPGPLEIGFDYCFLI
                     PATGDRVPCVYVENHRVVGLDPADPIRVSYGAKIGSDPTGRENPELLKMKLSRGHDQT
                     IVNGISRIGFMTGGRAARWVDEDMADTITGKAVKFVEENRRGPFFLFFSTHDIHVPRV
                     PHRRFAGQTFLGPRGDAIEQFDWCVGELLAALDRLKLAENTLVILSSDNGPVLDDGYA
                     DDAVEKLGAHQPAGPLRGGKYSLFEGGTRVPFLARWPARIKPGVSDALVSQVDFLATF
                     AELTGQTVAADAGPDSLPQLAALIGESKAGRDHLVEHANRLAIRKGQWKFFEPMPASG
                     KKAKPGKLATAAPEGALFNLKSDLGETKDVSAANPSVVSELTELLKKIRDGGRTRP"
BASE COUNT          889 a         1504 c         1538 g          747 t
ORIGIN      
        1 cgacgctgcg gctcgcgaag ttttgctaag ctgggtgaac tttgacgatg aacgcactgc
       61 cgtccccgac gaccttcatg caggaggcca tccgcctggc gatcgacggc gcccagcagg
      121 gacgcggcgg gccgttcgga gccgtcgtcg tgagggaggg gcagatcgtc ggtcgcggtt
      181 gcaattgcgt cacctcaacc aatgatccga cggcccacgc cgaggtcata gcgattcgcg
      241 acgcctgccg gcagctcgga gtctttcacc tggcgggctg cgagctctac gccagctgcg
      301 agccctgccc gatgtgcctg gctgccattt attgggcgca cctcgaccgc ttctattacg
      361 gcagcacggc ggcagaggcg gccgccgcgg ggtttgccga cgacttcatt cgccgagagc
      421 tgacgcgcga tccagagcag cgggcgattc ccagcgcccg cctgctagcc gccgaagcgg
      481 cggcggcgtt tgccgcctgg caggcgaagg ccgaccggat cgcatattga cgaaactcca
      541 gccgccgggc ggggttagag tgtcaggacc gcaacatgct cggccgcata ggacaggcgg
      601 caaagactgt tcctaggtgc cgagttggct tagatttaag gaaacgcgcc gcacggagcg
      661 accgcccacc tttgcacgcc ggagcgagcc gctgctttgg cactcaccca gtcccagcct
      721 atcaattacg aactgcacga tcctgacgtg cggctgatgc tccaagtccg cggcggcgac
      781 gccggggcct ttgaggaact ggtgctgcgc tatcagcacc ggctgctaac ggtgctggag
      841 cacctcgtgg ggaatcgcga gcaggcggag gatttgacgc aggaggtgtt tctgcgggtc
      901 tttcgggccc gcgaccggta cgagccggga gcgaagttca gcacgtggct gttcacgatt
      961 gcgaacaatg tcgccagcaa cgccaaccgc agccgatcgc gccgccgcga ggtaggcgtg
     1021 ccggagggga gcaacggctc ggaaagtgcc ccgctccagc tcgatcagct tgccaaggcg
     1081 gccagcagct tcatgccgac gcgggccctg gataaggccg agcaggccca gatggtgcgg
     1141 cacgcggtcg cggcgctgag cgagcggcag cggttggcgc tgctcttggc caagttcgag
     1201 gggatgagct accaggacat tgccgaaacg atgggcctgt cggtgcaggc gatcaagtcg
     1261 ctgctctcgc gggcccgagt gaacctgaag gagattctga cgccgtacgt cgagcagggt
     1321 accccgcccg acgaaaacaa gccgccgccc cagggcggct ccaagccccc ggccgatgat
     1381 gaatgacgcg acctcacgca tcgtacgaat agagagtcga gtgagccctt tatgagtgac
     1441 gaaaatcctt ccctcgccga agccagcacg atcgacgaag agctggtcgc ctatctcgac
     1501 ggcgagctca acaccgatca gatttcgcgc atcgagcggc ggctgctgga cgatccgcag
     1561 tatcgcgccc ggctgaacca gctggagagg acctgggacc tgctcgatac gctcaatcgc
     1621 tcgcaggccg acgacgactt cgcccacagc accgtcgaga tggtggccgt acaggcggtc
     1681 gaagaagcca agaccgagac gctgcggacg atccggcggc ggaacctggg ctgggtggcg
     1741 atcgtggccg cggtgctggc cctcacgggc gtgacttacg gcgtcattca gaatcgcctc
     1801 gcgcagccca accgcgagct ggtccgcaac ctgccgctga tcgagcgcgt ggacgactac
     1861 cgcaacatcg acagcctgga atttgtcaag gagctggaaa aggccaacct ctttgccgcg
     1921 gaggtggaca gtggggactg agtggatccg cggaacttct tccgcaggac ggcccttctg
     1981 ggccatcatt gagggcccag aagggccgtc ctacggcttg aatcaatgcg cgcggccact
     2041 tagctggatt gcgccgatcc tggcgctgtt ggctttcggg ctgcccgccg cggcggtcgc
     2101 cgacgagacc gaggccgagc gggctgctcg catcaaccag atgtcaaccg aggacaaagc
     2161 gctgctggcg ggcaagaaga agcggttcga cgagctgaag ccgccggagc tggcccacct
     2221 gcagggcctg tacgaagcca tctcaagcga cgcggaagcg caaaagcacg aagagacgat
     2281 ccgccgctac aacaagtggc tggccgacct atcgtcgggc cagcgcggca agatcctgga
     2341 tatcaaagat cccaaggagc ggattgacaa gatcaaggag ctcatgcgca agcaggagga
     2401 agagcgcttc cagcagttcg tcgagctgga cgtcaccaat gctgatcgcg ataccgttta
     2461 cgcctggctg gaggcattta tcgttcagca cgaggaccag atcatgacca ggctgcgcga
     2521 ggaagcgcgg cggcgcattc aggaggcgcc tgacgaagcc gcccgccgca aggccttgat
     2581 tgacaggtgg caaagtgcga gatggtatgt ccctgacatg ccattcccca cgaaggacga
     2641 cttcaatcaa ctcttcgcga agctctcgga cgaagccaag acgcggatca ccgccggcgc
     2701 taacaccgac gagcaagagc agcgactgac tgacttgatg cgggctgcta tcgtcagccg
     2761 cgtattttcg cagcccagtc gcgaggactt gcaaaaattc tatgcgaaca tgaagcccga
     2821 cgacccgcgc cgcgagagac tggaaggatt ggatcgcgag aagttgtacc agcaactacg
     2881 agccatgtat taccgggagc ggttcccagc cggctttcgc ggtcgcggcg gtcctggctc
     2941 gcgcggaggc ggaccgccgt tcgcgccgcc cggcgaaggt ggcggccagc ggccgccgcc
     3001 gaaaggcgag cggcctccgg ccgaaggcaa tgcctccggc gccccgccac cggctggcaa
     3061 gcagccctga agcgatcgat ttcttgcctg ggagcggatt catgggcgac accggtcggc
     3121 tgctcgtgcg attttttttg agtgtcttgc tgctcgcggg ggctgcctcg gccgctgagc
     3181 ggccgaatat cgtgctgatc tacgccgatg acctcggcta tggtgacgtc ggctgctacg
     3241 gcgcggagtg ggtcaagacg ccgcacatcg accggctggc gagggaaggc ctgcggttca
     3301 ctgacggcca ctgcgcgtcg gccacctgta ctccgtcgcg gtacgcgctg ctcaccggcc
     3361 aatatccctg gcgaaaagcc ggcaccggca tcctgcccgg cgacgccgcg gcgatcattt
     3421 cgcctggcgc cccgacgatc gccagcacgc tgaaagccgc cggctacaaa acgggcgtgg
     3481 ttggcaagtg gcatctgggc ctgggcagcg gcaatctcga ctggaacggc gagatcaagc
     3541 cgggaccgct agagattggc ttcgactatt gcttcctgat tcccgcgaca ggcgatcgcg
     3601 tcccctgcgt ctatgtcgag aaccatcgcg tcgttggcct cgatccggcc gacccgatcc
     3661 gcgtcagcta cggcgcgaag atcggcagcg atccgaccgg ccgcgaaaac cccgagctct
     3721 tgaaaatgaa gctgagccgc ggccacgacc agacaatcgt gaacggaatc agccggatcg
     3781 gcttcatgac cggcggccgg gcggcccgct gggtcgatga agacatggcc gacacgatca
     3841 ccggcaaggc cgtgaagttc gtcgaggaaa accgccgcgg gccgttcttc ctcttcttct
     3901 cgacgcacga catccacgtg ccccgcgtgc cgcaccggcg gtttgccggc cagacgtttc
     3961 ttggcccgcg cggcgacgcg atcgagcaat tcgactggtg cgtcggtgag ttgctcgctg
     4021 cgctcgaccg gctgaagctc gccgagaaca cgctggtgat tctcagcagc gacaacggcc
     4081 cggtgctgga cgatggctat gcggacgacg ccgtggaaaa actcggcgct caccagccgg
     4141 ccggaccgct tcgcggcggc aagtacagcc tcttcgaagg cggcacgcgg gtgccgtttc
     4201 tcgcccgctg gcccgcgcgg atcaagcccg gcgtctccga cgccctcgtc agccaggtcg
     4261 atttcctggc aacgtttgcc gaactgactg gccaaacggt cgccgccgat gccggccccg
     4321 acagcctgcc gcagctcgcg gcgctcatcg gcgaatcgaa ggccggccgc gaccacctgg
     4381 tcgagcacgc caaccgcctg gcgatccgca aggggcagtg gaaattcttc gagccgatgc
     4441 cagccagcgg caaaaaggcc aagcctggca agctcgcgac ggctgcaccc gaaggcgcgc
     4501 tgtttaatct gaagtccgac ctcggcgaga cgaaggacgt ttcggccgcg aatccctcgg
     4561 tcgtttcgga actgacggag ctgctcaaga agattcgtga cggcggccgc acccggcctt
     4621 agccgcctac caggaattca acaatgatgc gcctgtttgc cgccgcgatc gtgtccct
//
LOCUS       DASRJB010000107         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1724915, whole genome shotgun sequence.
ACCESSION   DASRJB010000107 DASRJB010000000
VERSION     DASRJB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724915"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFB80_03210"
     CDS             <1..435
                     /locus_tag="VFB80_03210"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32793.1"
                     /translation="AELMVTLAPQLNGLDIFRKNGFEFTAHPGSYVPPPLVGIWARWP
                     YFHNNSVPNLDELLKPAAERVSIYFVGTADNLQHDYDAAAVGFPAGAKTPAEWRTAGR
                     LFDTRRGGLSNSGHDEGIFVTGGKSDLAPEEKAALIEFLKTL"
     gene            complement(432..4076)
                     /locus_tag="VFB80_03215"
     CDS             complement(432..4076)
                     /locus_tag="VFB80_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZN32794.1"
                     /translation="MAGLLRAEPPKTAAEASDYQRTSTYAEVTAFCDELAKQSPRVRL
                     GTLGTSQEGRKLPLVIVADPPVATAAEAASSRKLVVFALANIHAGEVDGKESLLMLAR
                     DLALAPDKSLLDNLVIVFAPIFNADGNEKLGPHRPAQAGPTQVGTRENAAGLDLNRDF
                     VKLESPEARALVKFIGAWNPAVTIDCHTTNGSHHRYTLTYEGGRHPAGDPRIVQFTRE
                     KLLPEAGRLLKEKTGFESYFYGNFAADHTRWETILPLARYGTHYIGLRNRIAVLSESY
                     SYAPFKDRVRASQEFVRGVLQAASENRTEIRRLLAQPESAARIPLQYKAAPLGEPADI
                     PGFALEEKDGRRVATTTPRDYKAQYLGGTESTLVVARPFAYLLPASLTKVVENLQRHG
                     LTVEELREDLDLEIEAYRIDRVSRGRAFQRHELVELEATVRKETRRIAAGTVLVKTAQ
                     PLGSLAATLLEPQSADGLATWNFLDEQLKEGGEFPILRLPAAAVMTAGRVRLLAEDRT
                     FNKPITYDAVYGGGFGRGLSFGGSPASIQAWLDDGEHFLQQKEGQLYRVEARTGKASK
                     YIDEDALARGLAKLPAMSQQAAQGLSRGAWQRLTPQRDAALFNHQGDLYYCKLDGSGA
                     VRLTRTPAAEELASFSPDGKLVAFVQANNLFVVDVATQTVRPLTTDGSATITSGKADW
                     VYYEEIFNRDYRCYWWSADSSRIAFLRLDDGPVRNFTIVDQSSARPEPEVTPYPKAGA
                     PNPLASLGLVAVAGGEPQFVDLSNYTPTASLVIRAGWLPDNQTAYFYVQDRAQTWLDF
                     CTAAPGGETKCLFRETTKTWVNDPGPPHFLKDGSFLLTSERDGWRHYYHFAADGKLIR
                     PLTSGEWEARTLHTVDEAGGWVYFSGTRDSHLGANLYHVKLDGTQLERLTAGGGTHTC
                     SVSPKSNLFVDVWSDHQTPTQVRLCESDGKLARTLDTNPVYSLEEYRRGSYRLVQIPT
                     SDGYTIEASLLVPPDFDSTRRYPVWFTTYGGPHAPTIGDSWAGGRVRDEMLAQLGFVV
                     FKADPRSASGKGECSTWTAYRRLGVQELKDVETAIGWLKQQPGIDGERIGMSGHSYGG
                     FLTAYCLTHSKLFAAGIAGAPVTDWKSYDSIYTERYMNTPQENPQGYSDTSVVRAARN
                     LHGKLLILHGLIDDNVHVQNSLQLIDALQRADKDFEVMFYPKARHGIGGAHYNRLQVE
                     FMKRALKVAP"
BASE COUNT          643 a         1361 c         1395 g          732 t
ORIGIN      
        1 gccgagctga tggtcacgct cgccccgcag ctcaacgggc tcgacatctt ccgcaagaac
       61 ggctttgagt tcacggctca tcccggctcg tacgtcccgc cgccgctggt aggcatctgg
      121 gcccgctggc catacttcca caacaattcg gtgccgaacc tcgatgagct gctcaagccg
      181 gccgccgagc gggtgagcat ctacttcgtg ggcacggccg acaacctgca gcacgactac
      241 gacgcggcgg ccgtcggctt cccagccgga gcaaagacgc ccgccgagtg gcgcaccgcc
      301 ggccgcctgt tcgacactcg ccgcggcggc ctttccaaca gtggccacga cgaaggcatc
      361 tttgtcaccg gcggcaagag cgacctggct cccgaggaaa aggcggcgct gattgagttc
      421 ctgaagacgc tttagggcgc gactttgagt gcccgcttca tgaactccac ttgcagccgg
      481 ttgtagtggg ccccgccgat gccgtggcgg gccttgggat agaacatgac ctcgaaatcc
      541 ttgtcggctc gctgcagggc gtcgatgagc tgcaggctgt tctgcacgtg gacgttgtcg
      601 tcgatcaggc cgtgcaggat cagcagcttc ccatgcaggt tccgcgccgc ccggacgacc
      661 gacgtgtcgc tatagccctg cgggttttcc tgcggcgtgt tcatgtaccg ctcggtgtag
      721 atcgagtcat agctcttcca gtcggtgacc ggagcgcccg cgatgccggc ggcaaacagc
      781 ttgctgtgcg tcaggcaata ggccgtaagg aagccgccat agctgtggcc gctcatgccg
      841 atccgctcgc cgtcgatgcc cggctgctgc ttgagccagc caatcgccgt ctcgacgtcc
      901 ttcagctcct gcacgccgag gcggcggtag gccgtccagg tcgagcactc tcccttgccg
      961 ctggcgctgc gcggatcggc cttgaacacg acgaacccaa gctgggcgag catctcgtcg
     1021 cgcacgcggc cgccggccca gctgtcgccg atcgtcgggg cgtgggggcc gccgtaggtc
     1081 gtgaaccaga ccggatacct ccgggtggag tcgaagtccg gcggcaccag caggctggct
     1141 tcgatggtgt agccatcgct cgttggaatc tgcaccagcc ggtagctgcc gcggcgatat
     1201 tcctccagcg aatagaccgg gttggtgtcg agcgtgcggg ccagcttgcc gtcggactcg
     1261 cagaggcgga cctgtgtcgg tgtctggtgg tcgctccaga cgtcgacgaa caggttgctc
     1321 ttggggctga cgctgcacgt atgagtgccg ccgccggccg tcagccgctc gagctgcgtt
     1381 ccgtcgagct tgacgtggta gagattcgcc ccgagatggc tgtcgcgggt gccggagaaa
     1441 taaacccagc cgccggcttc atcaacggtg tgcagcgtgc gggcctccca ctcgccgctg
     1501 gtcaggggcc ggatcagctt cccatccgcc gcgaagtgat aatagtgccg ccagccgtcg
     1561 cgctcgctgg tcagcaggaa cgaaccgtcc ttcaggaaat gaggcgggcc gggatcgttg
     1621 acccaggtct tggtcgtctc gcggaacagg cacttcgtct cgccgccggg cgcggccgtg
     1681 cagaagtcga gccaggtctg ggcccggtcc tgtacgtaga agtaagcggt ctggttgtcg
     1741 ggcagccagc cggcccgaat caccagtgag gccgtcggcg tgtaattcga cagatcgacg
     1801 aattgcggct cgccgccggc aacggccacc aggcccagcg aagccagcgg gttgggcgct
     1861 ccggccttgg gatagggcgt gacctccggc tcgggccggg cggacgactg atcgacgatc
     1921 gtgaaattcc ggaccgggcc gtcgtccaac cgcaggaacg cgatccgcga ggaatcggcg
     1981 ctccaccagt agcagcggta gtcgcggttg aaaatctctt cgtaatagac ccagtcggcc
     2041 ttgccgctag tgatcgtcgc gctgccgtcg gtcgtcagcg ggcggacggt ctgtgtggcg
     2101 acatcgacca cgaacaagtt gttggcctgc acgaaggcca cgagcttgcc gtcggggctg
     2161 aaggacgcca gctcttcggc ggcaggcgtc cgcgtgaggc ggaccgcacc gctgccgtcg
     2221 agcttgcagt agtagaggtc gccctggtgg ttgaagaggg ccgcgtcgcg ctgcggcgtg
     2281 agccgttgcc aggccccgcg ggaaagcccc tgggccgcct gctggctcat ggccggcaac
     2341 tttgccagcc cgcgagcgag cgcgtcctca tcgatatact tgctcgcctt gccggtgcgg
     2401 gcctcgaccc tgtagagctg cccttccttc tgttgcagga agtgctcgcc gtcgtcgagc
     2461 cacgcctgga tcgacgcggg cgagccaccg aagctcagcc cgcggccaaa gccgccgcca
     2521 tagacggcgt cataggtgat cggcttgttg aacgtgcggt cctcggccag cagccgcacg
     2581 cgaccggcgg tcatgacggc agcggccggc agccgcagaa tggggaattc gccgccttcc
     2641 ttcagctgct catccaggaa attccaggtc gccaggccgt cggccgattg cggctccagt
     2701 agcgtcgcgg ccaggctgcc cagcggctga gctgttttca ccaggacggt gccggcggcg
     2761 atccgccgcg tttccttgcg aacggtggct tccagctcga ccagctcgtg ccgctgaaag
     2821 gcccgcccgc ggctgacgcg gtcgattctg taggcttcga tctcgagatc caaatcctcg
     2881 cgcagctcct cgacggtcag gccgtgccgc tgcaagttct cgacgacttt ggtcaggctc
     2941 gcgggcagca ggtaggcaaa cggccttgcg acgaccagcg tagattccgt tccgccgagg
     3001 tattgggcct tgtagtcgcg cggcgtggtc gtcgcgactc gccggccgtc cttttcctct
     3061 agggcgaagc cggggatgtc ggccggctcg ccaagcggcg cggctttgta ttgcagcggg
     3121 atccgcgcgg ccgattccgg ctgggcgagc agccggcgaa tctcggtccg gttctcggac
     3181 gccgcctgaa ggacgccgcg gacgaactcc tgcgaggccc gcacgcggtc tttgaacggc
     3241 gcatagctgt acgactcgga cagcacggcg atacggttcc gcaagccgat gtagtgcgtg
     3301 ccgtagcggg ccagcggcag gatcgtctcc caccgcgtgt ggtctgccgc aaagttgccg
     3361 tagaaatacg actcgaagcc ggtcttctcc ttcagcaggc ggccggcctc tggcaggagc
     3421 ttctcgcgcg tgaactggac gatccgcgga tcgcccgccg gatgccggcc gccctcatag
     3481 gtcagcgtgt agcgatggtg cgagccgttg gtcgtgtggc agtcgatcgt gacggccggg
     3541 ttccaagcgc cgatgaactt gacgagcgcc cgcgcctcgg gcgactcgag cttcacgaag
     3601 tcgcggttga ggtccagccc cgcggcgttt tcccgcgtgc cgacctgcgt cgggccggcc
     3661 tgcgcgggcc ggtgcggacc gagcttctcg ttgccgtcgg cgttgaagat cggcgcgaat
     3721 acgatcacca ggttgtccag cagcgacttg tccggcgcga gggccaagtc gcgggccagc
     3781 atgagcagtg attccttgcc atcgacttcg cccgcgtgga tgttggccag tgcgaagacc
     3841 acgagcttgc ggctactggc cgcctcggcc gccgtcgcca cgggcggatc ggccacgatc
     3901 accagcggca gcttgcgtcc ctcctggctc gtgcccagcg tgcccaggcg gacccgcggc
     3961 gattgctttg ccagctcgtc gcaaaacgcg gtcacttcgg cataagtcga agtccgctga
     4021 tagtcgctcg cctccgccgc ggtcttcggc ggctcggccc gcaagaggcc agccatcaac
     4081 aacaatgcca gcaaagcagt gaaatgtcgc acgcggttgg ctccggtgag g
//
LOCUS       DASRJB010000108         6186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_880804, whole genome shotgun sequence.
ACCESSION   DASRJB010000108 DASRJB010000000
VERSION     DASRJB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880804"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFB80_03220"
     CDS             complement(<1..917)
                     /locus_tag="VFB80_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN32795.1"
                     /translation="MVVWLFTWSWDADEHVVLALWVCSIVAGFARDRWWNRHGVIVAL
                     LLGCALPPAGYLACHFIVGLVWHVGSDAVRQWLLFTLVVLAFCGGTSAAIAVLFLRFM
                     RWIAGSGSRMRSVAFAGLGIVALALAGVGVLVFRAPPAWMPILVIPPIDVQGYDPYLP
                     YAISDDGRFLAIGQSPPLQTSGGTGWLRIWSVESGREITPPGLKTPLASALCLSPDGR
                     MLAIVHGGISIYDTASGRLIESLQNPGTHIWAPQPCRFSPDGKSLVMCTADMRDRRVF
                     VWNTTDWQLLKEQRLSKPSRPVIAKGRLWL"
     gene            complement(1047..1358)
                     /locus_tag="VFB80_03225"
     CDS             complement(1047..1358)
                     /locus_tag="VFB80_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32796.1"
                     /translation="MYRRYEVLIPPRFNDGNAVPADLIATTLAELEQQFGAFSCETQN
                     IQGRWSYGGQTYKDDLVRIFVDVPDVPANHQFFVDFKERLKTRFQQLDIWITTHAIEI
                     L"
     gene            complement(1391..1795)
                     /locus_tag="VFB80_03230"
     CDS             complement(1391..1795)
                     /locus_tag="VFB80_03230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN32797.1"
                     /translation="MATKRAAASPEAATPAEPSQKTDFTNLRNSQRYSFRGRASAVFL
                     PQTPDGSAEEWEVVTTDLSRSGVSIMHRKKLAQGQQIMLILNEAKQFVEVCWCCQVWP
                     NLYAAGCRFIDEPAGAAEHTMAADEQSGDGDS"
     gene            complement(1955..4957)
                     /locus_tag="VFB80_03235"
     CDS             complement(1955..4957)
                     /locus_tag="VFB80_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32798.1"
                     /translation="MHRFICVLLVVLLSSIAAAADWPTYRADAERSGFTREKLPAELS
                     PAWTYRPLHGPQPAWPRDDRMLFDRAFDVAVADGKLYFGSSADCRVMALDAATGQEKW
                     SYFTGAPVRFAPAVWKDRVYAASDDGYLYCLSAADGSLVNRWRGGPSDEMILGNGRMV
                     SRWPARGAPAIKDGIVYFACGIWQTDGVYLLALDAESGRVLWRNDEAGRIYMPQPHGG
                     ANAESGVSAQGYLVATADRLLVPTGRAVPAGFLLGGGKFLYYHLQANGHVGGTQTVAA
                     GGSFYNGGNAFNVATGATEAKLGAGSFAAFPGAVVHATKREVRVLKAVEKTAPDRKGV
                     PAKVLEHEVVWSLPGVDGSAAAIVAGETIFVGGGTTVTAVDAKSRQAVWQTEVDGVPY
                     GLAAAGSRLYVSTDRGTIYCFDGSRHAAPAVHSQSKQPSAIDSRTAAAADEIVRQSGV
                     KEGYCLDLDCGDGSLALALAERTNLQIYALARAAEDASRARQKLLAAGLYGVRVTVHQ
                     PDALKPPYGKYFADLVVSSASLASGPLAMSGENLPPALRPGGGVACTGRPGAMQVFTR
                     PKLAKTGSWTHQYSDLGNTSCSADELVQGQLRALWFRDVDLTMPQRHGRGHAPLFFEG
                     RMFVEGLGAVRAVDAYNGRNLWEFKLPGIQDAFNADHLAGTAVTGSNFCVASSGVYIH
                     DKQRCYRLDPATGEKLAEFAPPAGASGQSGAWGYIAASGSTLFGSLANPDHKVRYAYV
                     RADMSEMWGESKTFFALNTESGKLRWRYDAEHSIRNNAIAIGKGRVFLIDRPIAKEDE
                     WNPADASRKLPAVTKVEQPAGRVIALDAATGKQLWKSDASAFGTMLAYSAPHDALLMA
                     YQSTRFKLPSEVGGKLAVFRGATGQQLWEKDAKYVTRPLINDRTIYAQGGAWDLVTGD
                     SKPFVFERSYGCGQLAGSKHMLLFRSATLGYLDLTRGSGVENFGGIRPGCWINALPVG
                     GLVLVPDASAGCQCSYQNRSWMALAGDE"
     gene            5026..5325
                     /locus_tag="VFB80_03240"
     CDS             5026..5325
                     /locus_tag="VFB80_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32799.1"
                     /translation="MNRERDGNEGGGLAVIVVAGFLVVLLALCAGGGFYYVRHVQVLQ
                     MLEAQRAMLAAKEAEARRAEATAAAAAAEAQLRAIDPEVQSLERLERQEGIALPK"
     gene            5776..6045
                     /locus_tag="VFB80_03245"
     CDS             5776..6045
                     /locus_tag="VFB80_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32800.1"
                     /translation="MFRFTIREMLLLTVSIALGLGWIVERVHSIKLQSDVTHYEREAN
                     LSKMAIKTMHEDLDRIERALPPHGLTLVWSRDMRPSVEKLAPAGP"
BASE COUNT         1138 a         2002 c         1990 g         1056 t
ORIGIN      
        1 agccagaggc ggcccttggc aattaccggc cgggatggct ttgaaagtcg ctgctctttg
       61 agcagttgcc aatccgtggt gttccagaca aacactcgcc gatccctcat gtcggcagtg
      121 cacatgacaa gcgacttacc atccggggaa aagcgacaag gctgcggtgc ccaaatatgc
      181 gtgcctggat tctggagaga ctcgatcagg cgtccgctcg ccgtgtcgta gatcgagatc
      241 ccgccgtgga ctatggcgag cattcggccg tcgggggaaa ggcatagcgc gctggccagc
      301 ggggtcttga gtccaggagg cgtgatttcc ctccccgact cgacgctcca gatgcgcagc
      361 cagccggtgc caccgcttgt ctgcagaggc ggtgactggc caattgccag gaaccttccg
      421 tcgtcggaga tcgcataggg aaggtagggg tcgtatcctt gaacgtcgat tggaggaatg
      481 acaaggattg gcatccaggc tggcggtgct cggaagacca gcactccgac cccggcgagc
      541 gcaagtgcaa caatgccgag cccggcaaaa gcgaccgatc tcattcgaga tccgctacca
      601 gcaatccaac gcatgaagcg aaggaaaaga actgcgattg ctgccgaagt tccgccacaa
      661 aaggcaagca ccacgagcgt aaataacaac cattgcctaa ccgcgtccga cccaacgtgc
      721 cagaccaggc caactatgaa gtggcaagcc aaatagcccg ccggcggaag cgcgcagcca
      781 agcaacaaag caacaatcac accatgacgg ttccaccagc gatcacgagc aaagcctgca
      841 acaatgctgc acacccatag tgccagcacg acgtgttcgt cggcatccca cgaccaggta
      901 aacagccaga ccaccatcag gaccaaagct gtcgccagaa gcaggccaag caatgagaaa
      961 cgccaaggcg acaatctgcg agcggcgttt ggagattcca tagccaaacc gccgaatcaa
     1021 gattggctgg aagcaaatca gcttttctac aatatctcaa tcgcgtgcgt cgtgatccag
     1081 atgtcgagct gctggaagcg ggttttgagg cgctctttga aatcgacgaa gaactggtgg
     1141 ttggcgggca cgtcggggac atcgacgaag atccggacga ggtcgtcctt gtaggtctgc
     1201 ccgccgtagc tccagcggcc ctggatattt tgcgtttcgc aggaaaacgc gccgaactgc
     1261 tgctccagct cggcgagcgt cgtggcaatc aagtccgcgg gcacggcatt cccgtcgttg
     1321 aaccgtggcg gtatcagcac ttcgtagcgg cggtacatga ggccagccta ccgcgggcca
     1381 tcgcccacta ctacgagtcg ccgtcgcctg actgctcgtc cgcggccatg gtatgttcgg
     1441 cggccccggc gggctcgtcg atgaagcggc agccggcggc gtagaggttc ggccagacct
     1501 ggcagcacca gcagacctcg acgaattgct tggcctcgtt gaggatcagc atgatctgct
     1561 gcccctgcgc cagcttcttg cggtgcataa tgctgacgcc gctccgcgac aggtcggtcg
     1621 tcacgacttc ccattcctcc gccgagccgt ccggcgtttg aggcaggaac acggcgctcg
     1681 cgcggccccg gaaggaatac cgctgcgagt tgcgcaagtt cgtgaagtcc gtcttctggc
     1741 tgggttccgc gggcgtcgcc gcttcgggcg aggcagcggc acgtttggtg gccatcgtgc
     1801 tgctcctgga ttggattgcc acgataaacc tacgccatgc ggaccggagc gcgacaggcc
     1861 cgcggaaaac cgcaggctcc aggcgcgcgc cggccgtgcc ggaaatgcgg tctgtagcgg
     1921 ctcggcacgc cacagagggc gtgccctaca gcgcttactc gtcgcccgcc aacgccatcc
     1981 aggaccggtt ctgatagctg cactggcagc cggccgaggc gtcgggcacc agcaccaggc
     2041 cgccgacggg cagggcgttg atccagcagc cggggcggat gccgccaaaa ttctcgacgc
     2101 ccgagccgcg cgtcaggtcg aggtagccca gcgtcgccga gcggaacagc agcatgtgct
     2161 tcgagcccgc cagctggccg cagccgtagg atcgctcgaa gacaaagggc ttcgaatcgc
     2221 cggtgacgag gtcccatgcg cccccctggg catagatcgt gcggtcgttg atcagcggcc
     2281 gcgtaacata cttggcgtcc ttttcccaga gctgctggcc ggtcgcgccg cggaagacag
     2341 ccagcttgcc gccgacctcc gaggggagct tgaagcgcgt cgattgatag gccatcagca
     2401 gcgcgtcgtg cggcgcgcta taggcgagca tcgttccaaa cgcgctggcg tccgacttcc
     2461 agagctgctt gccggtcgcg gcgtcgaggg cgatcacgcg tcccgccggc tgctcgacct
     2521 tcgtcacggc gggcagcttg cgggaagcgt cggccgggtt ccactcgtcc tccttggcga
     2581 tcggccggtc gatcaagaac acgcggccct ttccaatcgc gatggcgttg ttgcggatcg
     2641 agtgctcggc gtcgtatcgc cagcgaagct tgccgctttc agtattcagg gcgaagaacg
     2701 ttttcgattc gccccacatt tcgctcatgt cggcccgcac ataggcgtag cggactttgt
     2761 ggtcgggatt cgcgagcgag ccaaagagcg tcgagccgct agcggcgatg tagccccacg
     2821 cgccgctctg gccgctggcg ccggcgggcg gagcgaactc ggcgagcttt tcaccggtcg
     2881 ccggatcgag ccggtagcag cgctgcttgt cgtggatata gacgccgctg ctggcgacac
     2941 agaagttgct gccggtgacg gccgttccgg ccaggtggtc ggcgttgaag gcgtcctgaa
     3001 ttcccgggag cttgaactcc cacaggttgc ggccgttgta ggcatcgacg gcccgcacag
     3061 cgcccaggcc ctcgacgaac atccgcccct cgaagaacag cggcgcatgt ccgcggccgt
     3121 gccgctgcgg catcgtcaga tcgacgtcgc ggaaccagag cgcccgcagc tggccctgca
     3181 ccaattcgtc ggccgagcag ctcgtgttgc cgaggtcgga gtactgatgc gtccagctgc
     3241 cggtcttggc gagcttgggg cgcgtgaaga cctgcatcgc gccgggccgg cccgtgcagg
     3301 cgacgccgcc gcccggccgc agcgccggcg gaagattctc gccgctcatc gccaggggtc
     3361 cactcgccag cgacgctgag gaaacgacga ggtccgcgaa gtacttgccg tagggcggct
     3421 taagggcgtc tggctgatgc acggtcaccc gcacgccgta gaggccggcc gccagcaatt
     3481 tctggcgagc acgcgacgcg tcctcggccg ccctggccag cgcgtagatt tgtaagttcg
     3541 tccgctccgc gagggccagc gccaggctgc cgtcgccgca gtccaggtcg aggcaataac
     3601 cttccttgac gccgctctgg cggacaattt catcggcagc agcagccgtg cgactgtcta
     3661 tggcgctggg ctgcttgctc tgcgagtgaa ccgccggcgc ggcgtgccgg ctgccgtcaa
     3721 agcaatagat cgtgccgcga tccgtgctga catacagccg gctgcccgcg gctgccaggc
     3781 cgtagggaac gccatcgacc tccgtctgcc acactgcctg gcgtgatttc gcatcgacgg
     3841 ccgtcacagt cgtgccgccc cccacgaaga tcgtctcacc ggccacgatc gccgcggcgc
     3901 tgccgtcaac gcccgggagc gaccagacga cctcatgctc cagcactttg gccggcacgc
     3961 ccttgcggtc gggcgcggtc ttttcgaccg ccttgagcac gcggacctcg cgcttcgtcg
     4021 catgcacgac ggcgcccgga aacgccgcga agctgcctgc gccgagcttg gcctcggtcg
     4081 cccccgtggc gacgttgaac gcgttgccgc cgttgtagaa gctgccgccg gcggcgacgg
     4141 tctgcgtgcc gccgacgtgg ccattggcct gcaagtggta atagaggaac ttgccgccgc
     4201 ccaagaggaa cccggcgggc accgctcggc ccgtcggcac cagcagccga tcggccgtcg
     4261 ccaccaggta gccctgggcc gagacgccac tctcggcgtt cgcaccgccg tgcggctgcg
     4321 gcatgtagat tcggcccgcc tcgtcgttcc tccacagcac gcggcccgat tcggcatcca
     4381 gggccagcag gtagacgccg tcggtctgcc agatgccgca ggcgaaatag acgatgccgt
     4441 ccttgattgc cggcgcgccg cgggccggcc agcgcgagac catccggccg ttgccgagga
     4501 tcatctcgtc cgacgggccg ccgcgccagc gattgacgag cgagccgtcg gccgccgaca
     4561 ggcaatacaa atagccgtcg tcgctggcgg cgtagacgcg gtccttccag accgccggcg
     4621 cgaaccgcac gggagccccg gtgaagtagg accacttttc ctgtcccgtg gccgcgtcga
     4681 gcgccatgac gcggcaatcg gccgagctgc cgaagtacag cttgccgtcc gccacggcga
     4741 catcgaaggc ccggtcgaac agcatccggt cgtcgcgcgg ccacgccggc tgcggcccgt
     4801 gcagcggccg gtaagtccac gccggcgaaa gttcggccgg cagcttctct cgcgtgaaac
     4861 cgctccgctc ggcgtcggcg cgatacgtcg gccagtcggc ggcagccgcg atcgatgaca
     4921 ggaggacgac tagcaagacg caaatgaatc gatgcatggt gcgtgagtct ggcttgactg
     4981 tggctttcca atcaggtgaa tcttacactg accggggagg gctttatgaa ccgcgagcga
     5041 gacggcaacg aaggcggcgg gctggcggtg atcgtcgtgg cgggctttct ggtggtgctg
     5101 ctggcgcttt gcgccggcgg cggattctat tatgtccggc acgtccaggt gttgcagatg
     5161 ctggaagccc aacgggcaat gttggcggcc aaggaagccg aggctcggag ggccgaggca
     5221 accgcggcgg ccgcggctgc tgaggcccaa ttgcgggcaa tcgaccccga agtccagtcg
     5281 ctcgagcgtc tggaacggca ggagggcatc gccctgccga agtaatcgcc ccgttcccaa
     5341 ccgcctgttc acgcgcaaaa tgctgaacag gccctaatcg ggtggtgtcg gggcaatgcc
     5401 gtgctccggc cctggaccaa gaagcatcga cgcaagtcat tgtatcaaaa caacttatca
     5461 ccaattacca gtctcgtcat tgacgttagg aacataagcc tttgaaggct cgggtttcgc
     5521 gtttgaacag ggcgctttcg cgcagttttt tgcgcacgga gggacggaaa tcgagagccc
     5581 agcaatgccc agtccagcag tcgccgctag tcgctcattt ggagccaatc ttcacttctt
     5641 tctaaacacc tgtccacaca taaggtttcg gccgaattcc aagaatcttg gctgcaaaga
     5701 gaattctctg gaaatggtcg cgacctggct cgcgcctccg gctgcgggtt aagcgatcga
     5761 gcaggagccg ctcccatgtt tcgctttacc atccgcgaaa tgctgctgtt gaccgtgagt
     5821 atcgccctgg ggttgggctg gatcgtcgag cgcgtgcaca gcatcaagct ccaaagcgat
     5881 gtcacgcact acgaacggga agccaatctg tcgaagatgg cgatcaagac catgcacgag
     5941 gacctcgacc ggatcgagcg ggccctgccg ccgcatggcc tgacgctggt ctggtcccga
     6001 gacatgcgcc ccagcgtcga aaagctcgcg cccgccggac cctgaacccg ggcgggctta
     6061 acccactatc cgccctcgtc gttagtgtcc ctggggcgtg aacgtaatgt cgtcgaccgt
     6121 cgcgttgacg cggacctttt cgactccttg ttccatgagc ggggctggat cgaagaaggc
     6181 gatggt
//
LOCUS       DASRJB010000109         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2289036, whole genome shotgun sequence.
ACCESSION   DASRJB010000109 DASRJB010000000
VERSION     DASRJB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289036"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFB80_03250"
     CDS             complement(<1..356)
                     /locus_tag="VFB80_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZN32801.1"
                     /translation="MRFCVRLIAAVLICCCLPAMADDLFPDKNLEEAVRQEVFEKRGK
                     KEPLVEADVLNISQVRGKGKKIANLAGLEKCKSLALLELDNNEISDLAPIKDLKLLQA
                     VYLANNKIASIEPLAGL"
     gene            637..2184
                     /locus_tag="VFB80_03255"
     CDS             637..2184
                     /locus_tag="VFB80_03255"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32802.1"
                     /translation="MGKLSTKAQIPYSLCPAQQRALDWFASALGVGSIFHFWSRAGLG
                     RTTVLRQLHAQLGGALVTVQDFVEAARLGHPLALEDALYQVLASALQKHDRVIVDDLH
                     IAMAVMSGCHFYPRSGLFDSHLEVLAALAAESGKKLIVGTNGQLADPLRERAFGFGID
                     AFAPEDYRHLCEVFLGPPAAELDFGKVHRFAPKLNAHQLKSACAWQAQAGELATDSFI
                     DYLRSQELTSNVALGEVTQVELRELQGVDDVIRSLEANIVLPLENDEVATRLGLKPKR
                     GVLLVGPPGTGKTTVGRALAHRLKGKFFLVDGTFISGTEQFYRMIHQVFQYAKDNAPS
                     VIFIDDSDVIFESGQEHGLYRYLLTMLDGLESKSAGRVCVMMTAMDVGNLPPALIRSG
                     RIELWLEMRLPDEAARAGILAQHLAVAPTPLDQADIEQLAAATGGFTGADLKRTVEDG
                     KALYAFDLVAGSAIRPITEYFLAAAEAVQASKSRYAEAEARANANRPTRPVWFNPFSS
                     FDLADDN"
BASE COUNT          401 a          787 c          740 g          419 t
ORIGIN      
        1 aggccggcca gcggctcgat gctggcgatc ttgttattcg ccaggtagac cgcctggagc
       61 agcttgaggt ccttgatcgg cgccagatcg ctgatctcgt tgttgtccaa ttccagcagg
      121 gccagactct tgcacttttc caggccggcc aggttggcga tcttcttccc cttgccgcgg
      181 acctgcgaga tgttcagcac gtccgcctcg accagcggct ctttcttccc gcgcttttca
      241 aagacctcct gccgcacggc ttcttccagg ttcttgtcgg ggaacagatc gtcggccatc
      301 gccggcaggc agcagcaaat cagcacggcg gcaatcaatc gaacgcaaaa cctcatcgga
      361 agcctcaaca ggtgggagag cggcggggaa acggcgacca acctgcctca gattactttc
      421 ccccgccgcc atcttccacc cccgcacgcg tgtattgagg cctggattgc cgctatcgtt
      481 taacccggag ccggaggcga caggggccaa tcgggaagtt gcctgggcct ctggaccaag
      541 ccacaaccag gccctattgc cgcgaaccag tcctgccgcg gcgtgtctgg catcggactc
      601 ctttgcggcg gcttccgccg cttaactgac cgattaatgg gaaagctatc taccaaagca
      661 cagattccgt attcgctttg ccccgcccag caacgggcgc tggactggtt cgcgtcggcg
      721 ctcggcgtgg gcagcatctt tcatttctgg tcgcgggctg gcctggggcg gacaacggtc
      781 ctgaggcagt tgcacgcgca gctcggcggg gccctcgtca cggtgcagga cttcgtcgaa
      841 gccgcccgcc tggggcatcc gctggccctg gaagacgccc tctaccaggt gctcgccagt
      901 gccctgcaaa agcacgaccg cgtgatcgtc gatgacctgc acatcgccat ggccgtgatg
      961 agcggctgcc atttctatcc gcgctccggt ctgttcgatt cgcacctgga ggtgctggcg
     1021 gccttggcgg ccgagtcggg caagaaactg attgtgggca ccaatggcca actcgccgat
     1081 ccgctccgcg agcgggcgtt cggcttcggg atcgatgcgt tcgccccgga ggactaccgc
     1141 catttatgcg aggtctttct gggccctccg gcggcggagc tcgacttcgg caaggtgcac
     1201 cggttcgcgc cgaagctcaa cgcccaccag ctgaagagcg cctgcgcatg gcaggctcaa
     1261 gcgggcgagc tggcgacgga ttcgttcatc gattacctcc gctcgcagga gctgacgagc
     1321 aacgtcgccc tgggcgaggt aacgcaggtc gagctgcggg agctccaagg cgtggacgac
     1381 gtgatccgca gcctggaagc caacatcgtc ctgccgctgg agaacgacga agtggcgacg
     1441 cggctgggcc tcaagcccaa gcggggcgtg ctgctcgtcg gcccgccggg cacgggcaag
     1501 acgaccgtcg gccgggcgct cgcccatcga ctcaagggca agttcttcct ggtcgatggc
     1561 acgttcatct ccggcaccga gcagttctac cgtatgatcc accaggtctt tcaatacgcc
     1621 aaggacaatg ctccctcggt catcttcatc gacgacagcg acgtgatctt cgagagcggc
     1681 caggaacacg gtctgtaccg ctacctgctg acgatgctcg acggcctgga aagcaagagc
     1741 gccggccgcg tctgcgtgat gatgacggcg atggacgtcg gcaacctgcc accggccctg
     1801 atccgctccg ggcggatcga gctctggctg gaaatgcggc tgcccgacga agcggctcgc
     1861 gcgggcatcc tggcccagca cctggccgta gctccgacgc cgcttgacca ggccgacatc
     1921 gagcagctcg ccgcggccac cggcggcttc accggcgcgg acctgaaacg cacggtcgag
     1981 gacgggaagg cgctgtacgc cttcgacctc gtcgcgggga gtgcgatccg cccgatcacc
     2041 gaatattttc ttgcagccgc cgaggccgtc caagccagca agtccaggta tgccgaggcg
     2101 gaagcccggg ccaacgccaa tcggcccacg cggccggtct ggttcaatcc cttctcgagc
     2161 ttcgacctcg ccgacgataa ttagcgggcg aaccgctcgc tcacggcggt tgtcctgccg
     2221 ctcgccatgt tcccgctgcg catccgcctg aagaagaaca ccgacggcag ctcggcccag
     2281 agctgcacgc gggtcgatgg ttcggtcacc tggcagggct tgcggggcgg cacggcggcg
     2341 ttcttca
//
LOCUS       DASRJB010000110         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3982489, whole genome shotgun sequence.
ACCESSION   DASRJB010000110 DASRJB010000000
VERSION     DASRJB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3982489"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFB80_03260"
     CDS             complement(<1..973)
                     /locus_tag="VFB80_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32803.1"
                     /translation="MTPPAKSRWTVAIAAVLALSGGLAAQQPRSEEQRLAELARQLDA
                     DEFLSREHATESLLKAGPLAFPALRSVLKDGSLEATSRAFYILRELGLAAKFEENDEA
                     WSLLNELAARKEVPTIARRAEATLAELTRRRSAQAQSELEALGAKIGRNDLNGLRADE
                     PISFVEIGPEFRGQAQDLWRLKWLIEVPQVVFAGEKATDEWIKRAAAMPGLEELHVYG
                     GRLTDAALAPLADHATLDQLGIYYTPLGDAALSVLEKLPKLSFVKLYGTQVTAKRAGE
                     LTAAAPGLAVDFRRGAFLGVGCSRLDGTCTISSVHLGSPAQKAGLAES"
     gene            complement(1076..2824)
                     /locus_tag="VFB80_03265"
     CDS             complement(1076..2824)
                     /locus_tag="VFB80_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HZN32804.1"
                     /translation="MSEPGKPSPTEGLKADSRYLRGEMAAELTDGLDHFGKSSETLLK
                     FHGTYQQDDRDERGAGGLDSDGKKKKSFIFMVRSKIPGGKLTSEQLLAELDLCDEVGN
                     TTLRITSRQGLQLHGVVKGNLKRAISRINEIQLSTLGACGDVNRNVMCCPAPYQRNIY
                     RQTQALADQLAAHFAPRTKAYHELWLTDSATGEKQLVGGGSQNGDGFDVEPIYGRVYM
                     PRKFKMAIGYTFDNCVDLYANDLGLLAVTQGDEIVGYNLLAGGGMGMTPSAKKTFPAV
                     AKRLCYVSPNEVLKAVEAVFKVQRDFGNREDRKLARLKYLIANWGVEKFKAKVEEYYG
                     GPLAPFRTDADVAGFNDHMGWDEQGDGRLFYGLNIENGRIKDTPEMRLKSAVREVCQT
                     LKPSVRLTSHQSLIFADVQPHDRSLLESILRAHGVKLTKEVSTVRRWSMACVAWPTCG
                     LSITESERALPGIIDQIEAELARLGLPNERFTIRMTGCPNGCARPYNCDVGLVGKARG
                     KYTVYLGGRLLGDRLNFVYKDMVPEEEVASTLAPIFAYFKHARENGETLGDFCLRKGA
                     ADLAAWSEANAAALVR"
     gene            3431..3754
                     /locus_tag="VFB80_03270"
     CDS             3431..3754
                     /locus_tag="VFB80_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calmodulin-binding protein"
                     /protein_id="HZN32805.1"
                     /translation="MIRRMLLAAAAVIVLGGLASTAGAQDRAYGRVWGGQYGRTDWER
                     FYHYPYVWYPQNFWGNEYYRSSEDLYFRYPPEMRVPVYNRQWHNYYPEGRRYHQGHHF
                     ILDVF"
     gene            complement(3828..4856)
                     /locus_tag="VFB80_03275"
     CDS             complement(3828..4856)
                     /locus_tag="VFB80_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZN32806.1"
                     /translation="MSESREEQRGAPSFNRRAWLIGGGAAAALAGAGILLDRFRGKEG
                     VCICKGQNYAGDLVTTINDALLAVGVDPESMPGKRVLLKPNLVEPTRDCPQMTTHPAM
                     ILAAADVFRRWGATVTVGEGPGHVRDTDMALAESGVGEALSSEKLPFADLNYQDVAFV
                     KNRGRRSKLDGFFFPQSVVHADLIVSMPKMKTHHWVGVTAAMKNLYGTIPGCKYGWPK
                     NVLHYRGIPETVVDINASLPKTIAIVDGIDCMEGDGPIMGSLKHMGLVIVGANPVAVD
                     ATVCRLMQVDPYAVPYLRLADGRLGPLNERLIEQVGEAWQPLASPFAVLDKPHLKDLV
                     RNRGVLVT"
     gene            complement(4853..>5590)
                     /locus_tag="VFB80_03280"
     CDS             complement(4853..>5590)
                     /locus_tag="VFB80_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32807.1"
                     /translation="IAALALLVHGRVEAARRAVAELAKMQGASGAVGIRAGEDAPGWP
                     TSLAIVAWSKVAAAGYAAHISRGASWLLANRGEVIARTPEFGHNTQLVGWAYAEQTHS
                     WVEPTALAVLALKAAGRSSDPAAREGVAVLLDRQLPGGGLNYGNTYVLGQLIRPHVQP
                     TGIALVALAGEQDSSGRLAKSAAWLRRSIGPETTPQSLAWALLGLRAQGALPSQADEW
                     LANAAGQSVTNTFKLALLALAAKGWPK"
BASE COUNT          931 a         1885 c         1764 g         1010 t
ORIGIN      
        1 cgctttccgc caggcccgcc ttctgggccg ggctgcccag gtggaccgag gagatcgtgc
       61 aggtgccgtc gagccgcgag cagccgacgc ccaggaacgc cccgcggcgg aaatcgacgg
      121 ccaggcccgg tgcggcggca gtcagctcgc cggcccgttt ggccgtcacc tgcgtgccgt
      181 acagcttgac gaagctcagc ttcggcagct tctcgagcac gcttagcgcc gcatcgccta
      241 gcggcgtgta atagatgccg agttggtcga gcgtggcgtg atcggccagc ggcgccaggg
      301 ccgcatcggt aagccggccg ccatagacgt gcaactcttc cagtcccggc atcgcggctg
      361 cccgcttgat ccactcgtcg gtcgctttct cgccggcgaa gaccacctgc ggcacttcga
      421 tcagccactt cagccgccac aggtcctgcg cctggccgcg gaattccgga ccaatctcca
      481 caaagctgat cggctcgtcg gcccgcagac cgttcaggtc gttgcgaccg atcttcgcgc
      541 cgagcgcctc taattccgat tgcgcctgag ccgaacgacg ccgcgtcaac tcggccagcg
      601 tggcctccgc ccgccgggcg atcgtcggca cttctttgcg agccgccagt tcgttgagca
      661 gcgaccaggc ctcgtcgttc tcttcgaact ttgccgccag ccccagctcg cggaggatat
      721 aaaacgcccg cgaagtcgct tccaggctgc cgtccttcag cacgctccgc agcgccggaa
      781 acgccaacgg tcccgccttg agcaggctct cggtggcgtg ctcacggctc aggaactcgt
      841 cggcgtcgag ctgccgggcc aattccgcca gccgctgctc ttcgcttcgc ggctgctgag
      901 ccgccagccc gccgcttagc gcaaggactg ccgcgatcgc aacggtccat cggcttttgg
      961 cgggtggagt catgtctcta atataacccc tggaaacgaa caccgcctcg tttgaccagg
     1021 gagcccgggc gacggctgta cgcgaaaccg cccaaggctc caggttagac gaagtctaac
     1081 gcaccagggc cgccgcattc gcttcggacc aggcggccag gtcggcggcg cctttgcgga
     1141 ggcagaaatc gcccagcgtc tcgccgtttt cgcgcgcgtg cttgaaatac gcgaatatcg
     1201 gggcgagcgt gctggcgact tcttcctcgg gcaccatgtc cttatagacg aagttgagcc
     1261 gatcgccgag caatcggccg ccgaggtaaa ccgtgtactt gccgcgggcc ttgccgacca
     1321 ggccgacgtc gcagttgtag ggccgggcgc agccgttggg gcagccggtc atgcggatgg
     1381 tgaagcgctc attgggcagc cccaagcggg ccagctccgc ctcgatctgg tcgatgatgc
     1441 cgggcagggc ccgctcgctc tcggtgatcg agaggccgca cgtcggccac gcgacgcacg
     1501 ccatgctcca gcggcggacc gtcgaaacct ccttggtcag cttcacgccg tgggcccgca
     1561 agatcgactc cagcagcgag cggtcgtgcg gctgcacgtc ggcgaagatc aggctctggt
     1621 gcgaggtgag gcggaccgat ggcttcagcg tctggcagac ttcgcggacc gccgatttca
     1681 gccgcatctc gggcgtgtcc ttgatccggc cgttctcgat attcaggcca tagaacaagc
     1741 gtccgtcgcc ctgctcgtcc cagcccatgt gatcgttgaa gccagccacg tctgcgtcgg
     1801 tgcggaacgg ggccagcggc ccgccgtagt attcctcgac cttggctttg aacttctcga
     1861 cgccccaatt ggcgatgagg tatttcaggc gggccagctt gcggtcctcg cggttgccga
     1921 aatcgcgctg gaccttgaac accgcctcca cggccttgag cacttcgttg ggcgagacgt
     1981 agcacagccg tttggcgaca gcgggaaacg tcttcttggc cgagggggtc atgcccatgc
     2041 caccgccggc cagcaggttg tagccgacga tctcgtcccc ctgggtgacc gcgagcaagc
     2101 ccaggtcatt ggcatacaga tcgacgcagt tgtcgaacgt gtagccgatg gccatcttga
     2161 atttccgcgg catataaacc cggccgtaga tcggctcgac gtcaaagccg tccccgttct
     2221 gggagcctcc gcccacaagc tgtttctcgc ccgtcgcgga gtcggtcagc cacaattcgt
     2281 ggtacgcctt cgtccgcggc gcgaagtgcg cggcgagctg gtcggccagg gcctgcgtct
     2341 ggcgatagat gttccgctgg tacggcgcgg ggcagcacat cacgttgcga tttacgtcgc
     2401 cgcaggcccc gagcgtactg agctggatct cgttgatccg cgatattgcc cgcttgaggt
     2461 tccctttcac aacgccgtgc agctgcaacc cctgccgcga cgtgatccgc agcgtcgtgt
     2521 ttcccacttc atcgcacagg tccagctcgg ccaggagctg ctcgctggtc agcttgccgc
     2581 ccggaatttt cgagcggacc atgaagatga acgacttttt cttcttgccg tcggagtcga
     2641 gaccgcccgc gccgcgctcg tcacggtcgt cctgctggta ggtgccgtgg aatttcagca
     2701 gcgtctcgct ggacttaccg aagtgatcga ggccgtcggt cagctccgcg gccatctccc
     2761 cgcgcagata tcggctgtct gctttaaggc cctcggtggg gctgggcttt cctggctcgg
     2821 acatcggcgg cgctatcctc gcggcaaaaa catcaccaag ttgactatca ttgtcaccta
     2881 tttatcggcg cagtcaagcg agtatagtga catgcccaac cgatccccac atcaaatcgc
     2941 ccagctcgcg gcgggcgttt cattcgacca aacttatcgc gaccaaccca cccgaaagca
     3001 ttgccgcgat cgcgcctcat ttgcgagaat cccgccgcaa ccccagacgg ctcaagggct
     3061 tgcggcgcga cgctccacca ccccataagt agttttaaat gagcgttaga gaacgccttg
     3121 gtgccacctc acgtgggtcg ctgccccagc ccccggcgcc aaactggcag gccgcggcgc
     3181 aaccgaaact taggtgcaaa tagcatacca atgttcagat tcgccgaagc taccgccggc
     3241 aaagtcccgg ctgcttcgcc agtctccccg attcgccacc gcgcccagca tgcgcaaggt
     3301 gccggttttg acggatattc cctcaatgcc ggatgccggg ccggtcgatg gttccgctag
     3361 ggcatgtgca tcgattccgc cctgattggc ctccgcaagg ccacgggcga ttgacccagg
     3421 ggaccatgcg atgatccgtc ggatgctgct cgcggccgcg gccgtgattg tgctgggagg
     3481 gttggcttca acagccggcg ctcaagaccg ggcctacggc cgggtgtggg gcgggcaata
     3541 cggccgcacc gactgggagc ggttctacca ctatccgtac gtctggtacc cacaaaactt
     3601 ctggggcaac gaatactacc gcagcagcga ggacctctat ttccgctatc cgccggaaat
     3661 gcgcgtcccg gtctacaacc ggcagtggca caactactac cccgaaggcc gccggtatca
     3721 ccaggggcac cacttcatcc tggatgtgtt ctaggcactt gtcagtgatc agtagtcagt
     3781 tgtcagtggg attcagctcc aactgacgac tgacaacgga ccactgacta agtcaccagc
     3841 acgccgcgat tccgcaccag atccttcagg tgcggcttgt cgagaacggc aaaggggctc
     3901 gccagcggct gccacgcttc accgacctgc tcgatcagcc gctcgttgag cgggccgagc
     3961 cggccgtcgg cgagccgcag gtagggcacg gcgtagggat cgacctgcat cagccggcag
     4021 accgtggcat cgacggccac gggattcgcg ccgacgatca ccagccccat gtgcttgagc
     4081 gagcccatga tcggcccgtc cccctccatg cagtcgattc cgtccacgat ggcgatcgtc
     4141 ttggggagcg aggcgttgat atcgacgacc gtttcgggaa tcccccgata gtgcagcaca
     4201 ttcttgggcc agccgtattt gcagccgggg atcgtgccgt agaggttctt catcgccgcg
     4261 gtgacgccga cccagtggtg agtcttcatc ttgggcatcg agacgatcag gtcggcgtgg
     4321 acgacgctct gcggaaagaa gaagccgtcc aactttgagc ggcggccgcg gttcttgacg
     4381 aaggcgacgt cctgatagtt gaggtcggcg aacggcagct tctcggagga cagggcctcg
     4441 ccgacgccgg actcggccag ggccatgtca gtgtcgcgga cgtggccggg gccttcgccg
     4501 acggtgacgg tcgcgcccca gcggcggaac acgtcggccg cggccaggat catcgcaggg
     4561 tgcgtcgtca tctgcgggca atcgcgggtc ggctccacga ggttcggctt cagcagcact
     4621 cgcttgcccg gcatcgactc cggatcgacg cccacggcta atagtgcgtc gttgatcgtg
     4681 gtgaccaggt cgccggcgta gttctggccc ttgcagatgc agaccccttc cttgccgcgg
     4741 aagcgatcca gcagaattcc cgcgccggcc agcgccgcag cagcaccgcc gccgatcagc
     4801 caggcccggc ggttgaacga gggcgcgcct cgctgctctt cgcgcgattc gctcattttg
     4861 gccagccttt cgcggcgagc gccagcaggg caagtttgaa tgtattcgtc acgctttgac
     4921 cggctgcatt ggccagccac tcgtcggctt gcgaaggtag cgcgccttgc gctcgcaggc
     4981 cgagcagcgc ccaggcgagc gactgcggcg tggtttccgg gccgatgctg cggcgcagcc
     5041 aggcggcgga cttcgccagc cggccgctgg aatcctgctc ccccgccaag gcaacgaggg
     5101 cgatccccgt cggctgcacg tgcgggcgga tcaattggcc gaggacatag gtgttgccat
     5161 agttaaggcc gccgccgggc agctggcggt cgagcagcac ggcgacgcct tcgcgagccg
     5221 ccggatcgct ggatcggccg gccgctttca gcgcgagaac agccagcgcc gttggttcga
     5281 cccaagagtg cgtctgctcg gcataggccc agccgacgag ttgcgtgttg tggccgaact
     5341 ccggcgtccg cgcgattacc tcgccgcggt tggccagcag ccagctggcg ccgcggctga
     5401 tatgcgcggc gtaaccggcg gcggcaacct tgctccaggc gacgatcgcc aaactcgtcg
     5461 gccagcccgg cgcgtcctcg ccggcgcgga tgccaactgc gccgctggcg ccctgcattt
     5521 tcgccagctc cgcgacggcc cggcgagcgg cttcaactcg gccatgcact agaagcgcca
     5581 atgccgcgat
//
LOCUS       DASRJB010000111         5641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_33868, whole genome shotgun sequence.
ACCESSION   DASRJB010000111 DASRJB010000000
VERSION     DASRJB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33868"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..493
                     /locus_tag="VFB80_03285"
     CDS             14..493
                     /locus_tag="VFB80_03285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN32808.1"
                     /translation="MLQRMLAGVACCTLVLLLSEVALAQDKEVPFPTLPKGAGKIDDG
                     APKKFTPTASGLKYRILRKGTGAMPKATDKVEVNYHGWLDDGKKFDSSYDRGESISFG
                     LNQVIKGWTEGMQLVGEGGMIELEIPSDLGYGDRGSPGAIPPKARLHFLVELIKVQK"
     gene            527..2035
                     /locus_tag="VFB80_03290"
     CDS             527..2035
                     /locus_tag="VFB80_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HZN32809.1"
                     /translation="MQHALVAGAGLCCALLLAANPLAAEDAFTFQHDNVLGTSLDLRV
                     FAAERERAERAEARVLAEIDRLAAIFSTYSDASEFSRWQRTPVGQSSRVSRELLDVLA
                     ASDKYRKLSGGAFQPAAQVLTALWSRCEDQQRLPLADELKSAVAGAQGPHWRLDFQKQ
                     IAERLSDAPLSLNAIATGYIIDRASEAALADGEISGIILNIGGDVRFCGPAAQLAEVA
                     DPLGADNAAPLARILVQDKAVVTSGGERRGFDILGRHYSHIFDPRTGQPAMHVLSSTV
                     VAPRTIDADALATICSVLPADESLALIDRLPDTACLLVTTDGRRLVSREWASLKPEFA
                     SAQAAESARPAFNGGMELKIDFEINNPGGGRYHRPYVAVWIEDADGFPVRTLTLWVAG
                     SRWVPDLRRWYKSDQVRRQVDKFDLVARAGSTRKPGQYSAIWDGRDGEDKLCPPGEYT
                     VYIEAAREAGTYQLIRQKIALGKEPLSQKLTGNIEIKAASLDYRRAAADKPQ"
     gene            1998..2699
                     /locus_tag="VFB80_03295"
     CDS             1998..2699
                     /locus_tag="VFB80_03295"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZN32810.1"
                     /translation="MTTAAPPPTSPSDDAEETDFGPGELDPRCPRPRRGFGQQFAYWI
                     RWLHIYLSLLAFTALLFFAVTGLTVNHPDWFGASTVVATTAEGTLPAGLVPSSGDGDA
                     GIDKLALVEFLRSQHAVRGALAELRADDSECTVAFKGPGYAADVTIDRASSHYDLQVS
                     RYGLVAILNDLHKGRDTGGGWSLLIDVAAVAMTVVSLSGLVLLFYLKRRLLPGLITAV
                     VGTVLVIVVYAMFVS"
     gene            complement(2814..4259)
                     /gene="dnaB"
                     /locus_tag="VFB80_03300"
     CDS             complement(2814..4259)
                     /gene="dnaB"
                     /locus_tag="VFB80_03300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZN32811.1"
                     /translation="MSTITRRADAPQRKPLLELTDRQPPFDREAEIGVLGSVVLLPDV
                     LDDIVQIVRPDDFYDDAHRKLFLHMTALHESNKKIDPTLLVDRLKGAGDFEAVGGMAY
                     LSKVINAVPNAAHATYYAEIVREKSTFRGLIYAATEILRDAYDQTQEAAHLLGQAEQK
                     IFSILDSRSDSRVQSLRDVVLDAMERLDARMAGTHAAGGCDYGYRELDGKTAGLHQGE
                     LVILAARPSMGKTALALNIAENVALGQKVPALFVSLEMSSLELADRLLCSVARVNGHR
                     LRNGTVSQEDRLKLVDTAEVLSRAPLFVDDSPSRTVTEIAAAARRIIRRHGGLGLIVV
                     DYLQLIEPDNPKDPRQEQVAKIARRLKGLAREMKVPIMCLAQLNRQTEAGKDNIPRLS
                     HLRESGAIEQDADVVLFVHREEYYSRGEEREQVAGQAQIIIAKQRNGPIGEVQLEWLR
                     DFTRFQDPVPERLKDFDQFNDRQMVDPAAGF"
     gene            complement(4299..4946)
                     /gene="rplI"
                     /locus_tag="VFB80_03305"
     CDS             complement(4299..4946)
                     /gene="rplI"
                     /locus_tag="VFB80_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZN32812.1"
                     /translation="MHRIKTQRIRREGTFKPLPKAGNGVQLLLIQSVDHLGKQGDVVT
                     VKRGYALNYLLPQGLATVANDHHKRMVEKHKARLHEIEKARLASLRQLSEAIGKQSVT
                     IEANANEEGHLYGSVNASEIAAALKAAGFHITTDQVRLEGPLKELGLYTVKIHLHAEI
                     DAELKVWVVPTVVNEPTTAEKTDKAEKTDKPKAEKGEKAKADKTEKAKPEKAEKK"
     gene            complement(5042..5527)
                     /gene="ssb"
                     /locus_tag="VFB80_03310"
     CDS             complement(5042..5527)
                     /gene="ssb"
                     /locus_tag="VFB80_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZN32813.1"
                     /translation="MASFNRVILLGNLTRDIEVRYLQSGMAVADVGLAVNDRRKNQAG
                     EWVEETTFVDVTMWGRTAEVAGEYLSKGSPILIEGRLKLEQWEKDGQKRSKLSVVCER
                     MQMVGSKGGGGGGRGQNQNYDSGGEDGDAPAGPPARSGGQRASSGPPAPPPPTEDEIP
                     F"
BASE COUNT          860 a         1831 c         1894 g         1056 t
ORIGIN      
        1 acggagacgt ggcatgctac agcgcatgtt ggccggcgtc gcatgttgta cattggtcct
       61 tctgctcagc gaggtcgcct tggctcaaga caaagaagtc ccgtttccga cgctgcccaa
      121 aggggcgggc aagatcgacg acggtgcgcc gaagaaattc acccccacgg cgtccggcct
      181 gaagtaccgc attctccgta agggcacggg ggcgatgccc aaggccacgg acaaggtcga
      241 ggtcaactat cacggctggc tcgacgacgg caaaaagttc gacagctcct acgaccgcgg
      301 cgagtcgatc tcgttcggcc tcaatcaggt gatcaagggc tggaccgagg ggatgcaact
      361 cgtcggtgaa gggggcatga tcgagctcga gattccctcg gacctgggat acggcgatcg
      421 cggctctccg ggcgcgatcc cgcccaaggc gaggctgcat ttcctcgtcg agctgatcaa
      481 ggtccaaaag tagagtagct ggcgcacctg gtgtgccgtc ccgcatatgc aacacgccct
      541 ggttgccggc gctggcctgt gctgcgcgct gctgctcgcc gccaatccgc ttgcggcgga
      601 agacgcgttc acgtttcagc acgacaacgt gctgggcacg tcgctcgacc tccgcgtttt
      661 tgccgccgag cgcgagcgag ccgagcgggc cgaggcccgc gtgctggccg aaatcgaccg
      721 gctggcggcg atcttcagca cctattccga tgcgagcgaa ttcagccgtt ggcagcggac
      781 gccagtcggc cagagcagcc gtgtctcccg cgagctgttg gacgtgctgg ccgccagcga
      841 caagtatcgc aaactttctg ggggggcatt tcagcccgcg gcgcaggtgc tgacggcgct
      901 ttggtcgcgg tgcgaggatc agcagcggtt gccgctggcg gatgagctga aatcggccgt
      961 cgccggagcc cagggcccgc actggcggct ggatttccag aagcaaatcg ccgagcgatt
     1021 gtccgatgcc ccgctcagcc tgaacgcgat tgccacgggc tacatcattg atcgcgcgag
     1081 cgaggcggcg ctggctgacg gcgagatcag tgggatcatc cttaatatcg gcggcgacgt
     1141 gcggttttgc gggccggccg cgcagctcgc agaagtcgcc gatccgctgg gcgccgacaa
     1201 cgccgcgccg ctggcgagga ttctcgtcca ggataaggcc gtcgtgacca gcggcggcga
     1261 gcggcgcggc ttcgacattc tgggccgcca ttactcgcat atcttcgacc cgcgcaccgg
     1321 ccagccggcg atgcacgtgc tcagctcgac ggtcgtcgcc ccgcgcacga tcgacgccga
     1381 cgcgctggca acgatttgca gcgtgctccc ggctgatgag agtctggcgt tgatcgatcg
     1441 cttgccggac accgcatgcc tcttggtcac gaccgacggc cggcgactgg tcagccgcga
     1501 atgggccagc ctgaagcccg agttcgccag cgcgcaggcg gcagaatcgg cccggccggc
     1561 gttcaacggc gggatggagc tgaagatcga cttcgagatc aacaatcccg gcggcgggcg
     1621 ctaccaccgg ccctacgtcg cggtgtggat cgaagacgcg gacgggtttc cggtccgcac
     1681 gctgaccttg tgggtggccg gctcgcggtg ggtgcccgac ttgcggcggt ggtacaagag
     1741 cgaccaggtg cggcggcagg tcgataagtt cgacctcgtg gcccgcgccg gctcgacccg
     1801 caaaccgggg cagtattcgg cgatctggga cgggcgggac ggcgaagaca agctttgccc
     1861 gccgggcgag tacaccgttt atattgaagc cgcccgcgag gcgggcacct accagttgat
     1921 ccgccagaaa atcgcgctcg gcaaagagcc gctgtcgcag aagctcaccg gcaatatcga
     1981 gatcaaggcc gcttcgcttg actaccgccg cgccgccgcc gacaagcccc agtgacgacg
     2041 cggaggaaac ggatttcggg ccgggcgaac tcgatccgcg ctgcccgcgg ccgcggcgcg
     2101 gcttcggcca gcagttcgca tactggattc gctggctgca catctatctc tcgctcctgg
     2161 cgttcacggc gctgctcttc ttcgccgtga ccggcctgac cgtgaatcac cccgactggt
     2221 tcggcgcgag cacggtcgtc gcgacgactg ccgagggcac gctgccggcc ggcctggtgc
     2281 cctccagcgg tgacggcgac gcgggcatcg acaagctggc actggtcgaa tttctccgca
     2341 gccagcacgc cgttcgcggc gcgctggccg aattgcgggc cgacgattcg gagtgcaccg
     2401 tcgccttcaa gggcccgggc tatgcggccg acgtcacgat cgaccgcgcc agctcgcact
     2461 acgacctgca agtgtcgcgc tacggcctgg tcgccattct caacgacctg cacaagggcc
     2521 gcgacaccgg cggagggtgg tcgctgctca tcgacgttgc ggccgtcgcc atgaccgtcg
     2581 tgtcgctcag cggcctcgtg ctgctcttct atctcaagcg ccggctgctg cccggcctga
     2641 tcacggccgt ggtcggcacg gtgctcgtga ttgtcgtgta tgcgatgttt gtttcgtgac
     2701 ttgtaggtcc cgcctgccgg gcgagacctg agcagcgcgg tgcgttaaaa attctcggga
     2761 acgtttctgg tcgctgcgct tccccaaagg ccccttccgg cagaaggggc ctactaaaag
     2821 cccgccgcgg gatcgaccat ctgccggtcg ttgaactggt cgaagtcctt cagccgctcg
     2881 ggcaccggat cctggaagcg agtgaaatcg cggagccatt ccagctgcac ttcgccgatg
     2941 gggccgttgc gctgcttggc gatgatgatc tgagcctgtc ccgcgacctg ctcgcgctcc
     3001 tcgccgcggc tgtagtattc ctcgcggtgc acgaacaaga cgacgtcggc gtcctgctcg
     3061 atcgcgcccg attcgcggag gtgcgaaagc cgcgggatgt tgtccttgcc cgcctcggtc
     3121 tggcggttga gctgggccag gcacatgatc ggcactttca tctcgcgggc cagccccttc
     3181 aggcggcggg cgatcttcgc cacctgctcc tgccgcgggt ccttgggatt gtccggttcg
     3241 atgagctgca agtagtcgac gacgatcagc cccagtccgc catggcggcg gataatccgc
     3301 cgggccgcgg cggcgatttc ggtcaccgtg cggctgggcg agtcatcgac gaacagcggc
     3361 gcgcgggaga gcacctccgc cgtatcgacc agcttgaggc ggtcctcctg cgagacggtg
     3421 ccgttccgca ggcggtggcc gttgacgcgg gccacgctgc atagcagccg atcggccagc
     3481 tctagcgacg acatctccaa gctgacgaac agcgccggca ccttctgccc caaggcgacg
     3541 ttttcggcga tgttcagcgc cagggcggtt tttcccatgc tcggccgggc cgcaaggatg
     3601 accagttccc cctggtgcag gccggccgtc ttgccgtcca gctcgcgata gccgtagtcg
     3661 cagccgccgg cggcgtgcgt gccggccatg cgggcgtcga gccgctccat cgcatccagg
     3721 acgacgtcgc gaagcgattg cacgcggcta tcgctgcggc tgtcgaggat cgagaagatc
     3781 ttctgctcgg cctgccccag caggtgggcg gcttcctgcg tctggtcgta ggcgtcgcgg
     3841 agaatctcgg tcgcggcgta gatcaggccg cgaaaggtcg atttctcgcg gacgatctcg
     3901 gcgtagtacg tggcgtgggc ggcgttagga accgcgttga tcaccttgct caggtaggcc
     3961 atgccgccga cggcttcgaa atcgccagcg cccttcagcc gatcgaccaa cagcgtggga
     4021 tcgatcttct tgttgctctc gtgcagggcc gtcatgtgca ggaagagctt gcggtgggcg
     4081 tcgtcgtaga agtcgtccgg ccgcacgatc tgcacgatgt cgtccagcac gtcgggcagc
     4141 agcacgacgc tgcccaggac gccgatctcc gcctcgcggt cgaacggcgg ctggcggtcg
     4201 gtcagctcta agagcggctt cctttgcggc gcgtcggcgc ggcgcgtgat cgttgacatg
     4261 cgatcctccg tgacgtgcag agctggaatc gaaggccgct acttcttctc ggccttttcc
     4321 ggcttggcct tctcggtctt gtcggccttg gccttttcgc ccttttccgc tttcggcttg
     4381 tcggtcttct cggccttgtc ggttttctcg gccgttgtcg gctcgttgac gacggtgggc
     4441 acgacccaga ccttgagctc ggcgtcgatc tcggcgtgca ggtgaatctt gaccgtgtac
     4501 aggccgagct ccttgagcgg gccttccagg cgaacctggt cggtcgtgat gtggaagccg
     4561 gcggccttga gggcggcggc gatctcgctg gcgttcacgc tgccgtagag gtgaccctct
     4621 tcgttggcgt tggcttcgat cgtgacgctc tgcttgccga tcgcctcgga cagctgccgc
     4681 agcgaggcca ggcgggcctt ttcgatttcg tgcaggcggg ccttgtgctt ctcgaccatc
     4741 cgcttgtggt ggtcgttggc gaccgtcgcc aggccctgcg gcagcaggta attcagggcg
     4801 tagccgcgct tgacggtgac gacgtccccc tgcttgccga gatgatcgac tgactggatg
     4861 agcaacagct gcacgccgtt gcccgccttg ggcaggggtt tgaaagtgcc ttcgcggcgg
     4921 atgcgctggg ttttgatgcg gtgcatgggg aggagtgagg ggtgagtggt cggtgaggta
     4981 gccgaactcg ccagagttcg gtccgaattc tggcgaattc ggctaacggc aattgagttt
     5041 tctaaaacgg aatttcgtct tcggtcggag gcggcggggc aggggggccg ctgctcgcgc
     5101 gctggccgcc gctgcgtgcc gggggcccag cgggcgcgtc gccgtcctcg ccgccgctgt
     5161 cgtaattctg gttctggccg cggccgccgc ctccgccccc cttgctgccg accatctgca
     5221 tccgctcgca aaccacgctc aacttggaac gcttctggcc gtccttttcc cactgctcga
     5281 gcttcagccg gccttcgatc aagatcggcg agcccttgct caaatattcg cccgcgactt
     5341 cggccgtgcg gccccacatc gtcacgtcca cgaacgtcgt ctcttcgacc cattcgccgg
     5401 cctggttctt gcggcggtcg ttgacggcga ggcccacgtc ggccacggcc atcccgctct
     5461 gcaaataccg gacttcgatg tcgcgggtca agttcccgag aaggattacg cggttgaaac
     5521 tggccatcgg tcacctcgct ggcggcggct gcaaacagtg aacaaacgat acaacgaagg
     5581 ctaacgcgcc gcgctgcctg ccgcaagcta cgccggctgg ctcgcggggg cggccggggc
     5641 c
//
LOCUS       DASRJB010000112         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2572132, whole genome shotgun sequence.
ACCESSION   DASRJB010000112 DASRJB010000000
VERSION     DASRJB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572132"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFB80_03315"
     CDS             <1..1275
                     /locus_tag="VFB80_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZN32814.1"
                     /translation="SVSAVRTDAGLIEAKSGPIAICVLTRNNKDQRWTEENAAEVLTS
                     KIARAVYDYFEPVARDSVASHELRQGANGELVQALQRTLNARAKPSPELSVDGDFGPG
                     TEAAVKKFQASNGLEASGVVGPETFKALGALIFADAPQPSPEEVNSKQLDKEPRESLA
                     GPPLTTCKSWIVADAKSGKTLFGKQETQKLDFASTTKMMTAYIVCELAKDDAKVLAEE
                     LTFSEAADKTSGSTADVRAGEKLSVGELLYGLLLPSGNDAATAFAEHFGPRFQEPGAS
                     GQEPAKSFIAEMNRRAAELGMTDTHYENPHGLPARGHQSTCRDLATLAKAAMANPHFR
                     EYVNTRQHGSKVTGPGGYERNIVWKNTNQLLGIEGYAGVKTGTTNAAGACLVSWGERD
                     GKELIVVVLGSTSGDARYTDARNLFRYAWSQP"
     gene            1277..1516
                     /locus_tag="VFB80_03320"
     CDS             1277..1516
                     /locus_tag="VFB80_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HZN32815.1"
                     /translation="MSALLRVPDLDLPGVSVRVSAWHVAVGQRVAEGERVVELTAGDV
                     TIDLPAPVSGVLIQHCANVDLPLAAGQTLARFRPQ"
     gene            complement(1431..2564)
                     /locus_tag="VFB80_03325"
     CDS             complement(1431..2564)
                     /locus_tag="VFB80_03325"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN32816.1"
                     /translation="MTAPSPMLHEAVDRHMDVPQPEQPAAEPSAPAESIPHEPAVPTS
                     LELPAEVQPLRIAWGYTTSIIGIHVLSLLALSPWYFSWTGVILGFLGLPGYGLLGITL
                     CYHRMLTHQGLVCPKWLEHTLAIIGVCNLQDTPARWVAIHRLHHRYADRRPDPHSPLV
                     NFFWSHMGWVMVEHREHSTVMVFEKYARDVLRDRFYLALERNLAWFWVYAAHALAYVV
                     IGTAVGRLLFGDWGRAVQFGGSLLVWGVFVRTVVAWHCTWSVNSFTHILGYRNYNTHD
                     DSRNHWLVAIVAHGEGWHNNHHADQRAAAHGHRWWEYDLTWVVIRLLEMVGLARHVVR
                     PRTWSDAHSGRIDPPATGGESGPASGRPPAAGQRWRSAGSAPH"
     gene            complement(2776..>3642)
                     /locus_tag="VFB80_03330"
     CDS             complement(2776..>3642)
                     /locus_tag="VFB80_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN32817.1"
                     /translation="SGISDADTGEVKLPNTEDVVKPKYPGGAAPDADGLGTRREQLAI
                     WMASRDNPYLPRAGTNRVWWHLFGRGLVEPVDDLAPLNPASHPELFVELTKYFVQSGF
                     DLRELYRTLANTSAYQRTSQWTEKAVPPEMYVHMPVKSLTAEQLYDSVNRVLHRKPTS
                     MMPNVNVDSPLLDPQRQAFLARLDTRGRNALEYQAGVLQALALLNGKDLAEATDPVNS
                     PLLGSIQAPFFGDDDRLKTLFLAALARNPTDEELKLCRDQLAKYSESDRGKAFSDILW
                     SLLNSAEFALNH"
BASE COUNT          643 a         1212 c         1206 g          581 t
ORIGIN      
        1 tcggtttccg cggtccgcac cgacgccggc ctgatcgagg ccaagagcgg cccgatcgcc
       61 atctgcgtcc tgacgcggaa caacaaggac cagcgctgga ccgaagagaa cgccgccgag
      121 gtgctcacga gcaagatcgc ccgcgcggtc tacgactact tcgagcctgt agcccgcgac
      181 tccgtcgcga gccacgagtt gcggcaaggc gctaacggcg aactggtgca agccctgcag
      241 cgcacgctga acgcccgcgc taagccctcg cccgaattgt cggtcgatgg cgatttcggt
      301 ccgggcaccg aagcggcggt gaagaaattc caggcgtcta acgggctcga agccagcggc
      361 gtcgtcgggc cggaaacgtt caaggctttg ggggcgctga tctttgccga tgcgccgcag
      421 ccgtcgcccg aggaagtcaa cagcaagcaa ctcgacaagg agccgcgcga gtcgctggcc
      481 ggtccgccgc tgacgacctg caagtcttgg atcgtcgccg acgccaaaag cggcaagaca
      541 ctcttcggca agcaggagac gcagaagctc gacttcgcca gcacgaccaa gatgatgacg
      601 gcctacatcg tctgcgagct ggcaaaggat gacgccaagg tgctggccga ggagctcacc
      661 ttctccgaag cggccgacaa aaccagcggc tcgaccgccg acgtccgcgc gggcgaaaag
      721 ctctccgtgg gcgagctgct gtatggcctg ctgctgccgt cgggcaacga cgcggccacg
      781 gcctttgccg aacactttgg accgcgattc caggagccag gtgccagtgg ccaggagcca
      841 gccaagagct ttatcgccga gatgaaccgg cgggcggcgg agctgggcat gaccgatacg
      901 cattacgaaa atcctcatgg cttgccggcc cgcgggcatc aatcgacctg ccgcgacctg
      961 gcgacgctgg ccaaggccgc gatggccaat ccgcacttcc gtgaatatgt caacactcgt
     1021 cagcacggca gcaaggtcac cggccccggc ggctacgagc ggaacatcgt ttggaaaaac
     1081 accaaccaac tactaggcat cgagggctac gcgggtgtaa agaccggcac gaccaacgcc
     1141 gccggcgcgt gcctggtctc gtggggcgaa cgcgacggca aggagctgat cgtcgttgtc
     1201 ctcggctcca cctccggcga cgcccgctac accgacgccc gcaacctctt ccgctatgcc
     1261 tggtcgcagc cttgaaatgt ccgcactcct tcgcgtgccc gatctggacc tgcccggcgt
     1321 gagcgtccgc gtctcggcgt ggcacgtggc ggtcggtcag cgggtcgcgg agggcgaacg
     1381 cgtcgtcgag ctcacggcag gcgacgtgac gatcgacctg cccgctccgg tcagtggggt
     1441 gctgatccag cactgcgcca acgttgacct gccgctggcg gccggccaga cgctggcccg
     1501 ctttcgcccc cagtagccgg cggatcgatc cgcccgctgt gggcatcgct ccaggtccgc
     1561 ggccgcacga cgtgccgcgc caggcccacc atttccagca gccggatgac gacccacgtc
     1621 aggtcgtact cccaccagcg gtgcccgtgg gccgccgccc gctgatcggc gtggtggttg
     1681 ttgtgccagc cctcgccgtg ggccacgatc gccaccagcc aatggttgcg gctgtcgtcg
     1741 tgcgtgttgt agttgcggta gccgaggatg tgcgtgaacg aattgaccga ccaggtgcag
     1801 tgccaggcga cgaccgtccg cacgaacacg ccccacacca gcaggctgcc gccgaactgc
     1861 accgcgcggc cccagtcgcc gaagagcagc cggccaaccg ccgtgccgat cacgacgtag
     1921 gccagggcgt gggcggcgta aacccagaac caggccaggt tccgctccag ggccagatag
     1981 aaccggtcgc ggagcacatc ccgggcgtat ttttcaaaaa ccatcacggt gctgtgctcg
     2041 cggtgctcga ccatcaccca acccatatgc gaccagaaga aattgaccag cggcgaatgc
     2101 ggatcgggcc gccggtcggc atagcggtgg tgcaggcggt gaatggcgac ccagcgggcg
     2161 ggcgtatcct gcaagttgca gacgccgatg atcgccagcg tatgctccag ccacttcggg
     2221 cagaccagcc cctgatgcgt gagcatccgg tgatagcaga gcgtgatgcc gagcaatccg
     2281 tagcccggca atcccaggaa cccgaggatc acgcccgtcc agctgaagta ccagggcgac
     2341 agcgcgagca ggctcagcac gtggatgccg atgatgctcg tcgtatatcc ccaagcgatg
     2401 cggagcggct gcacctccgc cggcaattcg agggaggtgg gaacggccgg ctcgtgggga
     2461 attgactccg ccggcgctga gggctccgcg gcgggctgtt ccggctgcgg gacatccatg
     2521 tgcctgtcga ctgcctcgtg cagcattggg ctgggcgcgg tcatcgactg tatcggcaac
     2581 ccgggctagc gggctggctt tgagtggcgg atgttccagt tgtagcgcgc cacgacgacg
     2641 cgcctttgcg accggattcg cagcgggcaa ccagaaaact gccggcgaac acatggcccc
     2701 gctacgaatt acctaactta tcccccgccc ggccccgccg ccagggtcaa aactgacatt
     2761 catcggcggt gtttgctagt ggttcagcgc gaactctgca ctattgagca gcgaccagag
     2821 gatgtcgctg aacgccttgc cccggtcgct ttctgaatac tttgccagct ggtcgcggca
     2881 gagcttcagc tcctcgtcgg tgggattgcg ggccagggcg gcgaggaaca gcgttttcag
     2941 gcggtcgtcg tcgccgaaga acggggcctg gatcgagccc agcagcgggc tgttcaccgg
     3001 gtcggtcgcc tcggccaggt ccttgccgtt gagcagggcc agggcctgca gcacgcccgc
     3061 ctggtactcc agggcgttgc ggccccgcgt gtcgagccga gccagaaacg cctgccgctg
     3121 cgggtcgagc agcgggctgt ccacgttgac gttgggcatc atgctcgtcg gcttgcggtg
     3181 caacacgcgg ttgaccgaat cgtagagctg ctccgcggtg agcgacttca ccggcatgtg
     3241 cacgtacatc tccggcggca cggccttctc ggtccactgg ctcgtccgtt gataggccga
     3301 ggtgttcgcc agcgtacgat acagctcgcg caggtcaaag ccgctctgga cgaagtactt
     3361 cgtcagctcg acgaacagct cgggatggct ggccgggttc agcggcgcca ggtcatctac
     3421 gggctcgacc agcccgcggc cgaaaaggtg ccaccagacg cggttcgttc cggcccgcgg
     3481 caggtacggg ttgtcgcgcg aggccatcca gatggcgagc tgctcgcggc gcgtgcccag
     3541 accgtccgcg tccggagcgg ccccgccggg atacttgggc ttcacgacgt cttcggtgtt
     3601 gggcagcttc acctcgccgg tgtcggcgtc gctgatgccg ct
//
LOCUS       DASRJB010000113         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2572309, whole genome shotgun sequence.
ACCESSION   DASRJB010000113 DASRJB010000000
VERSION     DASRJB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572309"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..336
                     /locus_tag="VFB80_03335"
     CDS             163..336
                     /locus_tag="VFB80_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32818.1"
                     /translation="MLMWRRLAMVIIGGSLCLALLIAVLWDRKAVIVNSSDSNADIRE
                     QMLLHTPKGTSGP"
     gene            complement(333..1019)
                     /locus_tag="VFB80_03340"
     CDS             complement(333..1019)
                     /locus_tag="VFB80_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZN32819.1"
                     /translation="MTPTASILADPFYCRPEAYSYFLEQLPSLHETDSLVRAAVAVSL
                     HALDDVNPDRIAQRLAVLGLRVQERARSRTDSAILANLHAVLFEEEGFAGNLERYYHA
                     LNSYLPVVLALIYKAVGEHAGLTIEGLNAPGHFLVRVRCDHRWMIVDPFFGGQLLTRD
                     EAFDRLDRVAGKKLPRYDEYLAPATHHHWLARLIGNLRQLFATEGRRDDLAAMTELSH
                     ALAGECLRAA"
     gene            1400..>4146
                     /locus_tag="VFB80_03345"
     CDS             1400..>4146
                     /locus_tag="VFB80_03345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN32820.1"
                     /translation="MEPAGSTIRPTPALLGEERPGSSVSPGVSRAASEIQSSGRVVVP
                     FAQLPPDASPDDQTVISKPAVTPPAGSAPLKTMAALGAALVGQRLEHYDLLEFVGGGG
                     MGAVFRALDTRLGRTVAVKVLSKDQTDDETIRRFRNEAQSAARLDHPNIARVYYVGED
                     RGWNFIVFEFIEGTNLREIVEKRGPLPLEDALHYTLQVAEALAHSSGRDVVHRDIKPS
                     NVLVTAGDQVKLVDMGLARLHQVQSSSDDLTASGVTLGTFDYISPEQARDPRSADVRS
                     DIYSLGCTLYYMLTGRPPFPDGTPVQKLIRHNSDEPPDVRTYRPDLPPRAAVLVSKML
                     AKRPSQRQQSAAELCAEILALGQQLGLPKVTQHGQVIVAAGPAEGQWRAAAWQVAAAV
                     AVLAASVILVDAYWPSPPATNASLPPPRLEAPSPAPGGNTSANSAAPAAPTPRVATTP
                     KSTQGTPNDAAAAAPTAAANSAAIGSASPDAVASLTSGLALPALEGAIGVEPIEAEVS
                     ISPEAARNGLSPPAAPAKISRLFVVRETPALVEPESEYVTSLAEACRRAAAHGLTEIE
                     LAFSGRLLERPLDILNQRLTLRAAPGARPVVVFQPPTTEQRQMLRLAGSSTSRLKAQG
                     IEFRMELPDEVSSGWSLAAINTGQTLELYDCVLTVDASRAPGQDQVAMIAVQRRRAAD
                     TMAMADPQMAMAQAAGIVMERCIARGDATLVAMNDETPLSLSWTQGLLATSRRLIESG
                     GSDSTARKYWETISIDLDSVTALCPQGLYLMQRRLGRSSQFAASVNTKQCILMTDPGV
                     PLIEYIGVRDLTSDDLECNGELNRYPRADMVFLRTVSGVAGDPPVEAPLNKGLSSEKR
                     AAISIPWLHPLRTDVPLHELTKSDFALDAAMSGGGGAGFDLGQLPDAAGPQSEDPAPP
                     P"
BASE COUNT          678 a         1367 c         1439 g          662 t
ORIGIN      
        1 aggcccgcgg atcgcacggc tgctggctgc ggcggccgcc gaccacagcc ggggctgcga
       61 ggagacgatg ggaatggaag cgtgctcagc gcggtgtcgc gccagcgaac cactgcctga
      121 tccggcacgg cggttgcaca atcgcctcgc ggaggacgat cgatgctcat gtggcgacgg
      181 ctcgcgatgg tgatcattgg cggctcgctg tgtctcgcat tgctcatcgc cgtcctgtgg
      241 gacaggaagg cagtgatagt taattcgtcc gattcaaatg cggacattcg cgagcagatg
      301 ctgcttcata cgcccaaggg gaccagcgga ccttaagccg cccggaggca ttcgccggcg
      361 agggcgtggc tgagctcggt catggcggcg aggtcgtcgc ggcggccttc ggtggcgaag
      421 agttgacgca ggttgccgat caggcgggcc agccagtgat ggtgtgtggc gggggcgagg
      481 tactcgtcgt agcgcggcag ctttttgccc gcgacgcggt ccagccggtc gaaggcttcg
      541 tcgcgggtga gcaactggcc gccgaagaac ggatcgacga tcatccagcg atgatcgcac
      601 cgcacgcgga ccagaaagtg gcccggcgcg ttgagccctt cgatcgtcag ccccgcatgc
      661 tcgcccaccg ccttgtagat cagggccagc acgaccggca ggtagctgtt gagcgcgtgg
      721 tagtaccgct ccaggttccc ggcaaaaccc tcctcctcga acagcaccgc gtgcaggttg
      781 gccaggatcg ccgaatcggt ccggctgcgg gcccgttcct gcacccgcag gcccagcacc
      841 gccagccgct gggcgatccg atcggggttc acgtcgtcca gggcgtgcag cgacaccgcc
      901 acggccgccc gcaccaggct gtccgtctcg tgcaggctcg gcagctgctc caggaaatag
      961 gaataggcct cggggcggca atagaacggg tcggcgagga tgctagcggt cggcgtcatt
     1021 tggcggttat ccggtggtgc gcgtagttcg ggcgggccgg tccgatcggc agcgccgtct
     1081 cttcataagt cgggaggccc gcaaaaatcc gggtaataaa tccttacgga atttcctttg
     1141 ccaatttgaa cacttgcgtc ggaacgtgcc gtaactgatt gtcggcattg actttaattt
     1201 gttgactgtg ggctggcggg ggactagaat cggcaaggcg ttgaaacgcc atctcccgtt
     1261 cgggcgattg tttttgggca atgatgtgcc cggtagacgt tcgtcagccg ttggagagta
     1321 aggaagctcc gcgaaaatcc gacccagcgc tgcttcgtcc ggggtttccg gtacggagcg
     1381 gctacgcgcc agaagccgta tggaacccgc tggttcgaca attcgcccta cgccggccct
     1441 cctcggggag gagcggccag gctcttcggt ttcgcccggc gtttcgcggg ccgctagcga
     1501 gattcaatcc agcggccggg tcgtggtgcc gtttgctcag ttgccgcccg acgcctcgcc
     1561 cgacgaccag acggtgatct ccaagccggc cgtcacgccg ccggcgggtt ccgccccgct
     1621 gaagacgatg gccgcgctcg gcgcggcttt ggtgggccag cggctggagc actacgacct
     1681 cttggagttt gtcggcggcg gcggcatggg cgcggtcttt cgggccctcg acacgcggct
     1741 gggccgcacg gtggcggtca aggtgctctc gaaggaccag accgacgacg agacgatccg
     1801 ccgcttccgc aacgaggccc agagcgcggc ccgcctggac cacccgaaca tcgcccgcgt
     1861 ctattacgtg ggagaagacc gcggctggaa cttcatcgtc ttcgagttca tcgaggggac
     1921 aaacctccgc gagatcgtcg agaagcgcgg gccgctgccc ttggaggacg cgctgcacta
     1981 cacgctgcaa gtcgccgaag cgctggcaca ttcatccggc cgcgacgtcg tgcaccgcga
     2041 catcaagccg tcgaacgtgc tggtcaccgc gggggaccag gtgaagctgg tcgatatggg
     2101 gctggcccgg ctgcaccagg tccagtcgtc gtccgacgac ttgaccgcca gcggagtgac
     2161 gctcggcacc ttcgactaca tctcgcccga gcaggcccgc gacccgcgct cggccgacgt
     2221 gcggagcgac atctactcgc tgggctgcac gctgtattac atgctcacgg gccggccgcc
     2281 gtttcccgac ggcacgccgg ttcagaagct gatccgccat aattcggacg agccgccgga
     2341 cgtgcggacg taccggcccg atctgccgcc gcgggccgcg gtgctcgtgt ccaagatgct
     2401 ggccaagcgg ccctcgcagc ggcagcaaag cgcggccgaa ctgtgcgccg agattctcgc
     2461 gctgggccag caactcgggc tgcccaaggt cacccagcac ggccaggtga tcgtggcggc
     2521 cggaccggcc gaggggcagt ggcgggcggc ggcgtggcaa gttgcggctg cggtcgcggt
     2581 tctcgctgcg tcggtcattt tggtggatgc ctactggccc agtccgccgg caacgaatgc
     2641 ctcgctcccg ccgccgcgct tggaggcgcc cagcccagcc ccgggcggaa ataccagcgc
     2701 caattcggct gccccggccg cgcccactcc gcgcgtggcg acgacaccga aatcaactca
     2761 gggcacgccg aatgacgcag ctgcggccgc tccgacggcc gccgcgaatt cagcggcgat
     2821 tggcagcgcc tcacccgatg ccgtcgccag cctcaccagt ggtttggcgc tgccggcgct
     2881 cgaaggcgca atcggcgtcg aaccaatcga ggccgaagtc tcgatcagcc cggaggccgc
     2941 ccgcaacgga ctttcgccac ccgccgcgcc ggcgaagatc agccgcttgt tcgtcgtgcg
     3001 cgagacgccc gcgctggtcg agccggagag cgagtatgtg acttcgctcg ccgaggcctg
     3061 ccgccgggct gcggcgcacg gcttgacgga aatcgaactg gcgttcagcg gccggctgct
     3121 cgaaaggccc ctcgacattt tgaaccagcg actgacgctg cgggccgcgc cgggcgccag
     3181 gccggtcgtc gtctttcagc cgcccaccac ggagcagcgg cagatgctcc ggctggcggg
     3241 cagcagcacc agccggctga aggcccaggg gatcgagttc cggatggaat tgcccgacga
     3301 agtctcgtcg ggctggtcgc tggcggcgat caacacgggc cagacgctgg aactgtacga
     3361 ctgcgtgctg acggtcgatg cgtcgcgagc gccgggacag gaccaggtgg cgatgatcgc
     3421 cgtgcagcgg cggcgggcgg ccgacacgat ggcgatggcc gatccacaga tggcgatggc
     3481 ccaggcggcg ggcatcgtga tggagcggtg catcgcccgc ggcgacgcga cgctcgtggc
     3541 gatgaacgac gaaacgccgc tgtcgctcag ctggacgcag ggactcttgg ccacgtcgcg
     3601 gcggctgatc gaatcgggcg gcagcgattc gaccgcccgc aagtactggg agacgatcag
     3661 catcgacctc gattcggtca cggccctctg cccgcagggc ctgtatctga tgcagcggcg
     3721 gctgggccgc agcagccagt tcgcggcgag cgtcaacacg aagcagtgca tcctgatgac
     3781 ggacccgggc gtgccgctga tcgaatacat cggcgttcgc gatttgacct ccgacgacct
     3841 ggagtgcaac ggcgagctga accgctatcc gcgggccgac atggtcttcc tgcggacggt
     3901 ctcgggcgtg gccggcgatc caccggtcga ggctccactc aacaaggggc tctccagcga
     3961 aaagcgggcg gcgatcagca ttccctggct ccacccgctg cggaccgacg ttccgctgca
     4021 cgagctgacc aagtcggact ttgccctcga cgccgccatg tcgggcggcg gaggcgcggg
     4081 cttcgattta gggcagctcc cagatgccgc cgggccgcag tccgaggacc cagcgccgcc
     4141 gccgga
//
LOCUS       DASRJB010000114         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3418184, whole genome shotgun sequence.
ACCESSION   DASRJB010000114 DASRJB010000000
VERSION     DASRJB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418184"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VFB80_03350"
     CDS             complement(<1..2045)
                     /locus_tag="VFB80_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN32821.1"
                     /translation="MTHPTIVAAAFALWAATGFAADAIPIEAPLRSKPVDYAGEIAPL
                     FRAHCVACHNEKKAEGSLNLETPAAMRRGGENGPAIVSGKGAESLLLKVAAHQQETFM
                     PPADNAVGAVPLSPKELGVLKLWIDQGATGAAGARSVSFQPPAAGYQPILAVAVTPDG
                     QYAAASRGNQIHVYHLPTAKLVAKLVDPALGEAAHQDIVRCLVFGATGELLASGGFRE
                     VKLWRRPKLTPLAGEGEAPPAEADATTAKSPDGKTVATIVDGKAQLQDEDGKLLFELA
                     PDPRLLDQLRLLDAQIAFTKSAIELAKQDIKAYEGPQRRTMTTAEEVKKAEMEVMKAE
                     KTRDEKLAALEKAKADSKNVENAEKAAEDAKTAVNVAMTVVERAKAVAERAVKKLAEA
                     EQDVTAREERLKSLDADKTALQEKIKNAPASARSVAFWGEGRFVAIGTDEGAIHTYLA
                     ETGQPTETLVTGSGAVRALECAGNTLIATANEKRLRLDADPTWRLVRTIGGAAEPALL
                     VDRVLTVDFSPDGKLLATGGGVPSRSGELKIWNTTDGQLVREFAAPHADTVNAVRFAP
                     DGQQLASAGADRMVKVFDAADGELLKVMGGHTAAVTSLAWHSGGKQLVSAGSDQLLKQ
                     WDAETGLPTSTLKGTTYQIGAYKREVSAAAFVGDSEQFVAASGDGTVRLHRTTSDND"
     gene            complement(2125..>2354)
                     /locus_tag="VFB80_03355"
     CDS             complement(2125..>2354)
                     /locus_tag="VFB80_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32822.1"
                     /translation="TCYTTILAGGGVQRGIILGASDPLGELPKERPIGIQDVYATMYH
                     LLGIDYTKSYQNEANRPVQIINYGEPIAEIL"
BASE COUNT          312 a          798 c          796 g          448 t
ORIGIN      
        1 atgtcgttgt cgctggtcgt gcggtgcagc cggacggtgc cgtcgccaga ggccgcgacg
       61 aactgctcgc tgtcgccgac gaacgccgcg gctgagacct cgcgcttgta cgcgccgatc
      121 tgataggtcg tgcctttcag cgtggaagtc ggcaggccag tttcggcgtc ccactgcttg
      181 agcagctggt cgctgccggc gctgacgagc tgcttgccgc cgctgtgcca ggcgaggctc
      241 gtcacggccg cggtgtggcc gcccatgact ttcagcagct cgccgtccgc ggcatcaaat
      301 accttgacca tgcgatccgc gccggctgag gccagctgct ggccatcggg cgcgaagcgg
      361 acggcgttga ccgtgtcagc gtgcggcgcg gcgaactcgc gcacgagctg gccgtcagtg
      421 gtattccaga tcttcaactc gcccgaccgc gaaggcacgc cgccgccggt cgccaggagc
      481 ttgccgtccg ggctgaaatc caccgtcagc acgcggtcga ccaggagcgc cggctccgct
      541 gcgccgccga tcgtgcggac cagccgccag gtgggatctg catccagccg caggcgcttc
      601 tcgttcgctg tagcgatgag cgtgttgccg gcgcactcca gcgctcgcac ggcgccggag
      661 ccggtcacca gcgtctcggt gggttggccg gtttcggcca gataggtatg aatagcgcct
      721 tcgtctgtgc cgatggcgac gaatcgtccc tcgccccaaa aggcgaccga gcgggcactg
      781 gcgggagcat tcttgatttt ctcttgcagg gccgtcttgt cggcgtcgag cgacttcagc
      841 cgctcttcgc gggccgtcac gtcctgctcg gcctcggcca gctttttgac ggcccgctcg
      901 gcgacggcct tggcccgctc gacgacggtc atggcgacgt tgaccgcggt cttggcgtcc
      961 tcggcggcct tctcggcatt ctcgacgttt ttgctgtcgg ccttggcctt ctccagcgct
     1021 gccagctttt cgtcccgcgt cttctcggcc ttcatcactt ccatctcggc cttcttcacc
     1081 tcctcggccg tcgtcatcgt ccgccgctgc gggccctcgt aggccttgat gtcctgcttc
     1141 gccagctcga tcgcgctctt ggtgaaggca atctgcgcat cgagcagccg caactggtcg
     1201 agcagtcgcg ggtcgggggc gagctcgaag agcagcttgc cgtcttcgtc ttgaagctgg
     1261 gccttgccgt ccacgatggt cgcgacggtc ttgccgtccg gcgactttgc ggtcgtcgcg
     1321 tcggcttcgg ccggcggcgc ttcgccttcg ccagcgagcg gcgtcagctt tggccgccgc
     1381 cagagcttta cttctcggaa gccgcccgag gcgagcagct cgcctgtggc gccaaacacc
     1441 agacagcgga cgatgtcctg atgggcagcc tcgcccagtg ccggatcgac gagcttcgcc
     1501 accagcttgg ccgtcggcag gtgatagacg tgaatctggt tcccgcggct ggcggcggcg
     1561 tattggccgt cgggcgtcac ggccacggcg aggatcggct gatagccggc ggccggcggc
     1621 tgaaagctga cgctgcgggc cccggcggcg cccgtcgcgc cctggtcgat ccagagcttg
     1681 aggacgccca gctccttggg cgaaagcggc acagcgccga cggcgttgtc cgccggcggc
     1741 atgaaggtct cttgctgatg ggccgcgact ttcagcagca ggctctcggc acccttaccg
     1801 gaaacgatcg cggggccgtt ttcgccgccc ctgcgcatcg ccgcgggcgt ttccaggttg
     1861 aggctcccct cggccttctt ctcgttgtgg caggccacgc agtgcgcgcg gaagagcggg
     1921 gcgatctcgc cggcgtaatc gacgggtttg ctgcgcagcg gcgcttcgat cggaatcgcg
     1981 tcggcggcga agccggtcgc ggcccagaga gcgaatgcag ccgcaacaat cgtagggtgg
     2041 gtcaagcgag tccgcgagcg ctgacccacg cggcactgac gaggctcccg cgtgggtctt
     2101 cggccgcagc ggcctcgacc caccctacag aatctcagca atcggctcgc catagttgat
     2161 gatctggacg ggccggttgg cctcgttctg gtagctcttc gtgtagtcga ttcccagcag
     2221 gtgatacatc gtggcataga cgtcctgaat gccgatcggc cgctccttgg gcaattcgcc
     2281 cagcgggtcg ctggcgccga ggatgatccc gcgctggacg ccgccgccgg cgaggatcgt
     2341 cgtgtagcaa gtcg
//
LOCUS       DASRJB010000115         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2009415, whole genome shotgun sequence.
ACCESSION   DASRJB010000115 DASRJB010000000
VERSION     DASRJB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009415"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="miaB"
                     /locus_tag="VFB80_03360"
     CDS             complement(<1..1020)
                     /gene="miaB"
                     /locus_tag="VFB80_03360"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZN32823.1"
                     /translation="MTRRLYIETVGCQMNVLDSEMVVADLRKRGYELAADLAAADVIL
                     FNTCSVREQAENKTYSALGRLRSFKQRHPEKIIGVMGCMAQKDQQLVFSRAPYVDLVV
                     GPGQLARIPDLIEAVGHGKGRQMAVSLGRTDGPVAEIRRSHETFDPLRDPTMRPTPFQ
                     AYLRIQIGCDKFCTYCIVPMTRGPEQGRPPEQILAEARVLADQGCKEITLIGQTVNSY
                     KHASGGRTTRLSDLLYSLSEIGGVQRLKFVTNYPKDMTDDLLHAVRDLPQCSPYLHVP
                     VQSGSNAVLARMKRGYTVEDYREMLGRIRATIPHAAVTSDFIVGFCGETEADFQQTYE
                     LVKESR"
     gene            complement(1024..1221)
                     /locus_tag="VFB80_03365"
     CDS             complement(1024..1221)
                     /locus_tag="VFB80_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32824.1"
                     /translation="MPNMDVWSWVLLVTGAFVAVTTLVGLMRRRRDEVLAELTAQARE
                     EQHKKRLAEQEESRKKKKRAA"
     gene            complement(1309..>2964)
                     /locus_tag="VFB80_03370"
     CDS             complement(1309..>2964)
                     /locus_tag="VFB80_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32825.1"
                     /translation="ARIEQLEQDLANTGSETSRSWSEWLDLPRLQAEAHSPAPDVPAL
                     EAIAERFYCGQAGLEAEPFVAVRRELRGFLAAREYAAADDPPDLYRRRVLDLSESLAR
                     LGTAANNDDVHRAGLRLAWLEPLSDEGSRAAQAVRARFCTTNGVIQASGRAANLLLRR
                     DVAQQKNISEIVLGNYTWGVAFMQGHVSVAIVPSAASGALEIRLQGQAICPANVAERR
                     RVSVQSSAATSISASKRMSITDLGLQLAPASAACWTSVQIQDVDAPRRLLERIAWRRA
                     NRVVPDAEAAASRRAESQSAAQLDGEAQELLRGINDLYYQKVRAPLIRGDALPPLFQF
                     WSDASHLRLALAQYNQAQLAPASPPPLLPPELDLGGSAHHSLLNNLSESLLGGKTIED
                     QIWHDMLQILLGTPPRPLWIHDRAERWSVTLARQRPLDVRFESDRVHAVLRLARVTRG
                     AEQLEVPVEIEASFAPQITRDGPLLVRDGDLTIRCDDPDLHKFLRRKFGAILPPELAF
                     SGLAPPAGGTLGKLRQLKLVEFRSAGGWLSVGYQLTPAAAPGP"
BASE COUNT          451 a         1022 c         1001 g          490 t
ORIGIN      
        1 ccgcgactct ttaaccagtt cgtaagtctg ctgaaaatcg gcctccgtct cgccgcagaa
       61 gccgacgatg aagtcgctgg tcacggccgc gtgcggaatg gtggcgcgga tgcggccgag
      121 catctcgcgg taatcttcca ccgtgtagcc gcgcttcatc cgggccagca cggcgttgct
      181 gccgctctgc accggcacat gcaggtaggg cgagcactgg ggcaagtcgc ggaccgcgtg
      241 cagcaagtcg tcggtcatgt ccttcggata attcgtgacg aacttcagcc gctggacgcc
      301 gccgatttcg ctgagcgaat aaagcaggtc ggacagccgc gtcgtgcggc cgccgctcgc
      361 gtgcttgtag ctgttgaccg tctggccgat cagcgtgatc tccttgcagc cctggtccgc
      421 gagcacgcgg gcctcggcga gaatctgctc cggcggccgg ccttgctccg ggccgcgggt
      481 catgggcacg atgcagtacg tgcagaactt gtcgcagccg atctggattc gcaggtaggc
      541 ctgaaacggc gtcggccgca tcgtcggatc gcggagcggg tcgaacgttt cgtggctccg
      601 ccggatttcg gccaccgggc cgtcggtgcg gcccaggctc accgccatct gccgcccttt
      661 gccatggccc accgcttcga tcaagtccgg aatccgcgcc agctgccccg ggccgacgac
      721 caggtccacg tacggcgccc ggctgaagac gagctgctgg tccttctgag ccatgcagcc
      781 catcacgccg atgattttct ccgggtgccg ctgcttgaac gaccgcagcc ggcccaaggc
      841 cgagtaggtc ttgttctcgg cctgttcgcg gaccgagcag gtgttgaaca ggatcacgtc
      901 ggcggccgcc aggtcggcgg ccagctcgta gccccgcttc cgcaggtcgg cgacgaccat
      961 ctcgctgtcc aggacgttca tctggcagcc gacggtctcg atataaagtc gccgggtcat
     1021 cacctacgcc gctcgtttct tcttcttccg cgattcttcc tgctcggcca gccgcttctt
     1081 gtgctgctcc tcgcgggcct gggcggtcag ctcggcgagc acttcgtcgc gccgccgccg
     1141 catcaggccg accagcgtcg tcaccgcgac gaaggccccc gttaccagca ggacccagct
     1201 ccaaacatcc atgttcggca taggacaccc aatcgcgcta aaaaatcgcg gacccgttat
     1261 cttagcactc cgcggcgcga ctcggcgagg gcagattggc ggcggatttc acggaccggg
     1321 cgcggccgcg ggcgtcagct gatagcccac gctcagccag ccgccggccg agcggaactc
     1381 gaccagcttc agctgccgca acttgccgag cgtgccgccg gcgggagggg cgaggccgct
     1441 aaaggccaat tcgggcggaa ggatcgcgcc gaacttgcgc cgcaggaact tgtgcaggtc
     1501 cggatcgtcg cagcggatgg tcaggtcgcc gtcgcgcacc agcagcggtc cgtcgcgggt
     1561 gatctgcggc gcgaagctgg cctcgatttc aacggggact tcgagctgct ccgccccgcg
     1621 cgtcacgcgg gccaggcgga ggaccgcgtg aacccggtcg ctctcgaagc gcacgtccag
     1681 gggccgttgc ctggccaagg tgacactcca gcgctcggcc cggtcgtgaa tccacagcgg
     1741 ccgcggcggc gtgcccagga gtatctgcag catgtcgtgc cagatctggt cttcgatcgt
     1801 cttgccgccg agcagcgact cgctcaggtt gttcagcagc gagtggtgcg cgctgccgcc
     1861 caggtcgagc tcgggcggaa gcagcggcgg cggcgaagcc ggggcgagct gcgcctggtt
     1921 gtactgtgcc agggccagcc gcagatggct ggcgtcggac cagaattgaa acaggggcgg
     1981 cagggcgtcg ccgcggatca gcggcgcacg gaccttctgg tagtacaagt cgttgatgcc
     2041 cctgagcagc tcctgggcct cgccgtcgag ttgcgccgcg gactggcttt cggcccgccg
     2101 cgaagcagcc gcttcggcgt cgggaacgac gcggttggcc ctccgccagg caattcgctc
     2161 gagcagccgg cggggagcat ccacgtcctg aatctgcacg ctggtccagc aggcggcgct
     2221 ggccggggcg agttgcaatc ccaggtcggt gatcgacatc cgcttgctgg ccgagatcga
     2281 cgtcgcggcc gagctctgca ccgacacgcg gcggcgctcg gcgacgttgg cggggcagat
     2341 cgcctggccc tgcaaacgga tctccagcgc gccgctggcg gcgctgggca cgatggccac
     2401 cgagacgtgg ccctgcatga acgctacgcc ccaggtgtag ttgccgagca cgatctccga
     2461 gatgtttttc tgctgcgcca cgtcgcgccg gagcagcaaa ttcgccgccc gccccgaggc
     2521 ctggatcacg ccgttggtgg tacagaaccg cgcccgcacc gcctgcgccg cgcgagagcc
     2581 ctcgtcgctc agcggctcca gccaggccag ccgcagtccc gcccggtgga cgtcgtcgtt
     2641 gttggccgcc gtgcccagcc gcgccagact ttcactcagg tccagcacgc ggcggcgata
     2701 caagtcggga ggatcgtcgg cggccgcata ctcgcgggct gccaggaaac cgcgcaattc
     2761 gcggcggacg gcgacaaagg gctctgcctc caggccagcc tggccgcaat agaaccgctc
     2821 cgcgatcgcc tccaaggccg gcacatccgg cgcgggcgaa tgagcttcgg cttgcagccg
     2881 cggcaggtcg agccactcgc tccaggagcg ggaggtctca ctgccggtgt tcgccaggtc
     2941 ctgctcgagc tgttcgatcc gcgc
//
LOCUS       DASRJB010000116         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2573391, whole genome shotgun sequence.
ACCESSION   DASRJB010000116 DASRJB010000000
VERSION     DASRJB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2573391"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VFB80_03375"
     CDS             <1..1451
                     /locus_tag="VFB80_03375"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN32826.1"
                     /translation="VATALGDEKNGKLLDETLAGLLARHGFTGRIEEQLEARLGRRVN
                     PELAEIGRNLFFDPIMALRGDNSCAGCHAPQFGFADSQSIAIGIRNNNVVGHGRTGPR
                     NQRRSPTLLNSAFFPALMWNARFFAPTSDPFDNTLGFVFPLPEGVTRFPPGDDRFQTL
                     LAAQGHLPQTELVEMTGFAGVTGAEDIDESFYPFDDGVGIAVPGPDASGFRNEPIRSR
                     VLVELNDSAYGGLFGRVFPSVRRGEPIDFAMVGAAIAEFEFTLTFADAPLDRFARGQR
                     SAMTAAEKRGAVLFFGQANCVACHRASGLANEMFSDFANHRLGVPQLAPRFGLNSGNV
                     HFDGPHADEDFGAEQISGRKSDRYKFRTSPLRNVALAPTFFHNGCFTRLEDAIAHHLD
                     PVASAKGYDPNVAGVALDLHLSQTPVGKVLQDLDPLLELPPRLTAGEFRDLVQFVRTG
                     LLDERATPEQLLLMVPRKLPSGLPLHDFERGD"
     gene            1696..2424
                     /locus_tag="VFB80_03380"
     CDS             1696..2424
                     /locus_tag="VFB80_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32827.1"
                     /translation="MKRKLLALGLVLAAGTLLASGGHTILIDDFNDRDSEGWVETDFT
                     GGRGSFSAHTRSYVLKTGPIAVDDPSVGTLDADWEPSEDNPLYANGTLRARIRANTYG
                     TTVGLLMRDTHELEADYGFYGSTSFGTFYIEYFNFSENPDAPQTILAMADPEEFPFEA
                     DVDYILEARVVGDKLSLKAWREGDSEPSEPMLVVKDNRLTADVATGLATIAFFDPAPL
                     IEQGVERVRVNATVDDITFTPQGH"
     gene            complement(2460..>2657)
                     /locus_tag="VFB80_03385"
     CDS             complement(2460..>2657)
                     /locus_tag="VFB80_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32828.1"
                     /translation="ERVHSIKLQSDVTHYEREAGLSKLAIKTMHEDLDRIERALPPHG
                     LTLVWSRDMRPSVEKLAPAGP"
BASE COUNT          428 a          934 c          863 g          432 t
ORIGIN      
        1 ccgtcgcgac tgcgctgggc gacgagaaga acggcaagtt gctggacgag acgctcgccg
       61 ggctgctcgc gcggcacggc ttcaccggcc ggatcgaaga gcagctcgag gcgcggctgg
      121 gccggcgagt caatcccgag ctggccgaga tcggacgcaa tctgttcttc gacccgatca
      181 tggccctccg cggcgacaac tcctgcgccg gctgccacgc cccgcagttc ggctttgccg
      241 attcgcagtc gatcgccatc ggcatccgca acaacaacgt cgtcggccac ggccgcacgg
      301 gtccgcgcaa ccagcgccgc tcgccgacct tgctcaactc ggcgttcttt ccggccctga
      361 tgtggaacgc gcgattcttc gccccgacca gcgacccgtt cgacaacacg ctcggcttcg
      421 tctttccgct gcccgagggc gtgacccgct tcccgccagg cgacgatcgc ttccagaccc
      481 tgctcgccgc gcaggggcac ctgccgcaga ccgagctggt cgaaatgacc ggcttcgcgg
      541 gagtgactgg cgcggaggac atcgacgaga gtttctatcc attcgacgac ggcgtgggga
      601 tcgccgtccc cggccccgac gcgagcggct tccgcaacga gccgatccgc agccgcgtgc
      661 tggtcgagct gaacgacagc gcttacgggg ggctcttcgg ccgcgtcttt ccgtcggtcc
      721 gtcgcggcga gccgatcgac ttcgcgatgg tcggcgcggc gatcgccgag ttcgagttca
      781 cgctcacctt cgccgacgct ccgctcgacc gctttgcccg cggccagcgc agcgcgatga
      841 ccgcggccga gaagcgcggg gccgtgctgt tcttcggcca ggccaactgc gtcgcctgcc
      901 accgcgccag cggcctggcc aacgagatgt tcagcgattt tgccaaccac cgcctgggcg
      961 tgccgcagct cgcgccgcgg ttcggcctga atagcggcaa cgtccacttc gacgggccgc
     1021 acgccgacga ggattttggc gccgagcaga tcagcggccg caaaagcgac cgctacaagt
     1081 tccgcacctc gccgctgcgg aacgtcgccc tggcccccac gttcttccac aacggctgct
     1141 tcacacggct ggaagatgcg atcgcgcacc acctcgatcc ggtcgcctcg gccaagggct
     1201 acgatccgaa cgtcgccggc gtggcgctgg acctgcacct gagccagacg cccgtgggca
     1261 aggtgctcca ggacctcgat ccgctcctgg aactgccgcc gcggctgacg gctggcgaat
     1321 tccgcgacct ggtgcagttc gtccgcaccg ggctgttgga cgagcgggcc acgcccgagc
     1381 agctgctgct catggtgccg cggaagctgc ccagcggctt gccgctgcac gacttcgagc
     1441 gcggcgacta gcgccgccgc cttctgtttg cattctgccc gcaagtaggc cccttctgcc
     1501 ggaaggggcc tggagggagg gcagcgacca agaatgttgt ctggaattcg aactcaccgc
     1561 cctgtccagg tcccgccggg caggcgggac ctacatgcgg gaaccgccgc cggcgctcgg
     1621 tttgcgcttc cggtgggcgg ctggactgcc atcctgcaaa cccgccgcgc cgcgatggcg
     1681 caccggagaa tagtcatgaa acggaaactg ctggccctgg gcctggtcct cgccgccggt
     1741 acgctgctgg cgtcgggcgg ccacacgatc ctgatcgacg acttcaacga ccgcgactcc
     1801 gagggctggg tcgagaccga cttcaccggc gggcggggct cgttcagcgc ccacacgcgg
     1861 tcgtacgtgc tcaagaccgg gccgatcgcg gtcgatgacc ccagcgtcgg cacgctcgat
     1921 gccgactggg agccgtcgga agacaacccg ctgtacgcca acggcacgct gcgggccagg
     1981 attcgcgcca acacctacgg cacgacggtc ggactgctga tgcgcgacac gcacgagctg
     2041 gaagccgatt acggcttcta cggcagcact agcttcggca cgttctacat cgagtacttc
     2101 aacttcagcg agaatcccga cgcgccgcag accatcctgg caatggccga tccggaggag
     2161 ttcccgttcg aggccgacgt cgattacatc ctggaagccc gggtggtggg cgacaagctc
     2221 tcgctgaagg cctggcggga aggcgactcc gagccgtccg agccgatgct cgtcgtgaaa
     2281 gacaatcggc tcaccgcgga tgtggccaca ggcctggcga ccatcgcctt cttcgatccc
     2341 gccccgctca tcgagcaagg tgtcgaaaga gtccgcgtca acgcgacggt cgatgacatc
     2401 acgttcacgc cccagggaca ctaacgacga gggcggatag tgagttaagc ccgcccggtt
     2461 caaggtccgg cgggcgcgag cttttcgacg ctggggcgca tgtctctgga ccagaccagt
     2521 gtcaggccgt gcggcggcag ggcccgctcg atccggtcga ggtcctcgtg catggtcttg
     2581 attgccagct tcgacagccc ggcttcccgt tcgtagtgcg tgacatcgct ttggagcttg
     2641 atgctgtgca cgcgctc
//
LOCUS       DASRJB010000117         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3984141, whole genome shotgun sequence.
ACCESSION   DASRJB010000117 DASRJB010000000
VERSION     DASRJB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984141"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFB80_03390"
     CDS             complement(<1..888)
                     /locus_tag="VFB80_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZN32829.1"
                     /translation="MARRKLGLWLIGAKGGVATTVTLGLLALRRGLTKTTGLVSALPQ
                     FAGLDFAEWGDFTIGGHEIRETTLFAEAQQLVRGNHALDAELVGKLKGDLDKLDKNTR
                     SGTLVNVGATIEGLADPALRKQKDSPRAAIERVQADLSDFIKKHSLDQLLVVNLASTE
                     PAPEEELPAKWKDLSKLLDKRKCPLPASSLYAIAALDLGCPYVNFTPSLGSAPPALDE
                     LARERGTCHMGHDGKTGETLLKSVLAPMFAARHLEVQSWVGHNIFGNLDGKVLDDPAN
                     KRTKVRSKDRLLHQILGYSP"
     gene            1047..2240
                     /locus_tag="VFB80_03395"
     CDS             1047..2240
                     /locus_tag="VFB80_03395"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZN32830.1"
                     /translation="MLSNLPQAPPDAILGLNEAFKLDPNPRKINLSAGVYKDEQGNTP
                     ILACVKEAERRLFASEKSKGYLSIEGHPQYASRVQELLFGTGHEVLQSQRAVTAQTPG
                     GTGSLRVAADFLRKHFPQAKVWVSTPTWANHAAIFQAAGQTVSTYPYIDKEGRRLDFP
                     AMLAALRQAPAGDIVLLHACCHNPTGIDPTPTQWLAIAAVLRERGLLPLVDFAYQGFG
                     DGLAEDAVGLAALAQPGQELLVCSSFSKNFGLYGERVGALSVVAADSAAAQRALSQVR
                     VAIRTNYSNPPTHGAAIVAEVLGDAALRKSWEQELAAMRSRIHQMRKLFVETMKQKAP
                     KHDFSFLAEQRGMFSFSGLTSMQVDELRSKHAVYVVGSGGRINVAGMTRENLDPLCTA
                     IAAVL"
     gene            complement(2250..2885)
                     /locus_tag="VFB80_03400"
     CDS             complement(2250..2885)
                     /locus_tag="VFB80_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN32831.1"
                     /translation="MDEIAVNGQPRAGEIRILDDDREVLAFLLRAAGGLGRAVRGFDD
                     PDQFLQVDGPPPACLLVDWQLRGRDGLEVIAQCQRQWPDAPVILITGHATIPVAVTAM
                     RQGVVGVLEKPVLPAHLVREIQFALHQSQCRSAAEVERCRARRLIESLNEQELTVLKL
                     VVGGTPNKNIAGQLRVAMRTAEKYRRSVFDKLGVDSAPEATRIWVLANQEP"
     gene            3049..3483
                     /locus_tag="VFB80_03405"
     CDS             3049..3483
                     /locus_tag="VFB80_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZN32832.1"
                     /translation="MQLPRSWFVPALLLVAAGGLAVGYSLGQERKVNTRLYELRTYTT
                     LEGRLPALHKRFAEHTLKLFEKHGMKNEMYFVPTDPARKDNTLIYIVSHDNQEAADKS
                     WKAFQADPEWAKARDASEKDGKIVAKVERVFMTMAEYSPGSR"
     gene            3572..>6508
                     /locus_tag="VFB80_03410"
     CDS             3572..>6508
                     /locus_tag="VFB80_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32833.1"
                     /translation="MPGSAREPLEATVLPDLPAARGRSGARGWLLACGAFLLFVVCFA
                     LLLWGLGQLLARAGGARPSRSGSLLDHWETTDERLAAVSAALEAEQTGLNSAQHRELR
                     RFFDKVRDAIQPLNGTAFTALVDMEAAADRVLLHPASSRRSRSERRLVEQQMQLRFRG
                     PSGWNRLIIVHGERSADGEEALVYTIATGGDQPPAPLRWWLKRDGRSWRVCDWERIDK
                     GFSETARWALEGAIGNSAEMAAFHQALEGVHNAQTSIEAGLVALGSRDLLDAQSLDLP
                     PVVNDVVQLELAWAWERCARSDQVLAACDRIRDSRASPGVHYLRALAHWRREDFAAVL
                     ADAQQYRSIAGPHPHLLEVEADALEALGRRAEAAECWWKRLALTPDDADALAEYCRLA
                     GESASRAVGILRRAASPEEFALGAGRTAIYRDDDATLGVIERFFRELDPQAAALESLA
                     AWRCEQRQEHPLAAAHYRLAAERSADEQDRQRHFAQFLDAMAYAGRAAAGYQAADDPR
                     AALAHLTAGLEYDEAPIVIDDLPRLIALHAQRAPQDPRLKYLEGLVAMADGQFAAAEA
                     KFRSAAKAPAADPPEGHLAELARSKLVEAVYRQNRLAAAYQTATSPPGEVFRELALLC
                     QQDRNWAALEELIALHRTRAPADPWIDYFLARRAQAQGNAEAGLAAVQRAEAASDETL
                     RPTLGWLKNELLIQSGGIGQAYQGSSDQREAFRRLVTHLASEEDWDGVLELATLHAAA
                     APRRSATLYWRAAAHWHRGEYQELVDNLSPWPEDRVGRLEPGQLTELCDLLVRSLLRL
                     GKLPEAQAAADRARDEYGVEPPMVALKLAQGDKAAVLELIEHPRICRELFGRQLYRDR
                     ELAPLLSDGEVAALRRQQALALPSDHWSRRTALVLFFAEPLPAEQLDECAAGATDAAI
                     RISGRPDQKSLLWNLGSETVVLTAGSGPYCSCGQLPETLGADDPRRAALEQHSSWIAL
                     DLLPAAQ"
BASE COUNT         1070 a         2116 c         2288 g         1034 t
ORIGIN      
        1 cggcgagtag ccaagaatct ggtgcagcag ccggtccttg ctgcggacct tggtgcgctt
       61 gttggccgga tcgtcgagga ccttgccgtc gaggttgccg aagatgttgt ggccgaccca
      121 gctttgcacc tcgaggtggc gcgcggcgaa catcggcgcg agcacgctct tgagcagcgt
      181 ctcgccggtc ttgccgtcgt gccccatgtg gcaggtgccg cgctcgcggg ccagttcgtc
      241 gagggccggc ggcgcggagc cgagcgacgg ggtgaagttt acataggggc agccgaggtc
      301 cagcgccgcg atggcgtaca gcgagctggc cggcagcggg cacttcctct tgtccagcag
      361 tttcgaaagg tccttccact tcgcgggcag ctcttcctcc ggcgcgggct cggtcgaggc
      421 gaggttgacg acgagcagct ggtcgaggct gtgcttcttg atgaagtcgg agaggtccgc
      481 ctgcacgcgc tcgatggcgg cgcggggaga atccttctgc ttgcgaagcg ccggatcggc
      541 gaggccttcg atggtggcgc cgacgttaac gagcgttccg ctccgcgtgt tcttgtcaag
      601 cttgtcgagg tcgcccttga gcttgccgac gagctcggcg tcgagggcgt gattgccgcg
      661 gaccagctgc tgggcctcgg cgaagagcgt cgtctcgcga atttcgtggc cgccgatcgt
      721 gaagtcgccc cactcggcga aatcgagccc ggcaaactgc ggcagggccg ataccaggcc
      781 ggtcgttttg gtcagcccgc gccgcagggc caagaggccc agggtgacgg tcgtcgcgac
      841 gcccccctta gcgccgatga gccacaggcc gagtttgcga cgagccatca gacgtatctt
      901 gcgccgaggg tgggagaggc gggcctgttc gagcgggcag cggcggcgtt aggataattc
      961 gctttgccag tagtgtctag gagcgagccc gatcgccagc cgcagtgtcc tgccgactcc
     1021 cgctccccgc ttcccgaccc cgacccatgc tctcgaacct tccccaggct ccgcccgacg
     1081 ccatccttgg cttgaacgaa gcgttcaagc tcgaccccaa cccgcggaag atcaacctca
     1141 gcgccggcgt ctataaagac gagcagggca acacgccgat cctcgcctgc gtgaaggagg
     1201 ccgagcggcg gcttttcgcc agcgagaaga gcaagggcta tctctcgatc gaagggcatc
     1261 cgcaatatgc cagccgagtg caggagctgc tcttcggcac ggggcacgag gtcctgcaaa
     1321 gccagcgggc ggtgacggcc cagacgccgg gcggcaccgg ctcgctccgc gtcgcggcag
     1381 actttttgag gaagcacttt ccgcaggcga aggtctgggt cagcacgccg acctgggcca
     1441 accacgccgc gatcttccag gccgcagggc agaccgtgag cacctatccg tacatcgaca
     1501 aggagggccg gcggctggac ttcccggcga tgctcgccgc cctgcggcag gcgcccgcgg
     1561 gcgacatcgt gctgttgcac gcctgctgcc acaatccgac cggcatcgat cccacgccga
     1621 cgcagtggct ggcgatcgcc gcggtgctgc gcgagcgggg cctcttgccg ctggtcgatt
     1681 ttgcctatca aggcttcggc gatggcctgg ccgaagatgc ggtgggcctg gcggcgctgg
     1741 cccagccggg acaggagttg ctcgtttgca gctcgttctc aaaaaacttc gggctgtacg
     1801 gcgagcgggt gggagccctc agcgtcgtgg ccgccgactc cgcggcggcg cagcgggccc
     1861 tctcgcaagt ccgcgtggcg attcgcacga actacagcaa tccgcccacg cacggcgcgg
     1921 cgatcgtcgc ggaagtgctg ggcgatgcag cgctccgcaa gtcctgggag caggagctgg
     1981 ccgcgatgcg ctcgcggatc caccagatgc gaaaactgtt cgtcgagacg atgaagcaga
     2041 aagcgcccaa gcacgatttc tcattcctgg ccgagcagag gggaatgttc tcgttttccg
     2101 gtctgacgag catgcaggta gacgagctgc gcagcaagca cgcggtttac gtcgtcggca
     2161 gtggcgggcg gatcaacgtc gccggaatga cccgcgagaa tctggacccg ctctgcaccg
     2221 cgatcgcggc ggtgctgtag cgatgcaccc tacggctcct ggttcgcgag cacccagatg
     2281 cgcgtggctt cgggcgcgga atccactccc aatttgtcga agacggaccg ccggtatttt
     2341 tcggcggttc gcatcgcgac tcgcagctgc ccggcgatgt tcttgttggg agtgccgccg
     2401 acgaccagtt tcagcaccgt caactcttgc tcgttgagcg attcgatcag ccgccgggcc
     2461 cggcagcgtt ccacctccgc tgccgagcgg cactgcgact gatggagggc gaactgtatc
     2521 tcgcggacga gatgggccgg gagcaccggc ttttcgagga ctccgaccac gccttgccgc
     2581 atcgcggtga ccgcgaccgg aatcgtcgcg tggccggtga tcaggatcac cggagcgtct
     2641 ggccactgcc gctggcactg ggcgatcact tccagcccgt cgcgtccgcg cagctgccaa
     2701 tccacgagca ggcaggccgg cggcgggccg tctacttgca ggaattgatc ggggtcgtcg
     2761 aagccgcgaa ccgcacggcc cagtccgccc gcggcgcgca gcagaaaggc gagaacttcg
     2821 cggtcgtcgt cgagaattcg aatttcgcct gcccgcggct ggccgttgac cgcgatttca
     2881 tccatgtcca aactcccccc gcaaaccgcg ctcctgcggc ggtcgatcgg ccaatataac
     2941 atcggggcgg cgattggcta cccgccgtgt ggcagggaat tgcaaccggt gccgcgggcg
     3001 gcgataacag ccagagtgat ttcttccttc tgatcaggag caccctccat gcagctaccc
     3061 cggagctggt ttgtgcccgc gttgttattg gttgccgcgg gcggcctggc cgtcggctat
     3121 tcgctgggac aggagaggaa agtgaacacg cgactgtatg agctgcggac ctatacgacc
     3181 ttggagggcc ggctgccggc gcttcacaag cggttcgccg agcacaccct gaaactgttc
     3241 gagaagcacg gcatgaagaa cgagatgtac ttcgtcccca ccgatccggc gcggaaagac
     3301 aacacgctga tttacattgt ctcgcacgac aaccaggagg cggcggacaa gagctggaag
     3361 gcctttcagg ccgatcccga gtgggccaag gcccgcgacg ccagcgaaaa ggacggcaag
     3421 atcgtggcca aagtggaacg agtgttcatg acgatggccg agtattcgcc ggggagccgg
     3481 taggtcggta agtcggtggt cggtggatcg gtgagtccaa ccgaccgact gactgaccga
     3541 ccgaccgacg gacccgcttg ggggaacccg tatgcctgga tcggctcgcg aaccgctgga
     3601 agccaccgtg ctgcccgacc tgccggcggc ccgcgggagg agcggcgccc ggggatggct
     3661 gctggcttgc ggcgcgttcc tgctgttcgt tgtctgcttt gctttgctgc tgtgggggct
     3721 gggacagctg ctggcccgcg ccggcggcgc gcgacccagc cgcagcggct cgctgctgga
     3781 ccactgggaa acgaccgacg agcggctggc ggcggtgagt gccgcgctgg aagccgagca
     3841 gaccggcctg aattcggccc agcaccgtga attgcggcgg ttcttcgaca aggtccgcga
     3901 cgccattcag cccctgaatg gcacggcgtt caccgccctg gtcgatatgg aagctgcggc
     3961 ggaccgcgtt ctgctgcatc cggcatccag ccgccgctcg cgcagcgaac ggcggctggt
     4021 cgaacagcaa atgcagctcc gcttccgcgg gcccagcgga tggaaccggc tgatcatcgt
     4081 ccacggcgag cgttctgcag acggcgaaga agcgctggtc tatacgattg ccacgggcgg
     4141 ggatcagccg ccggcgccgc tgcggtggtg gctgaagcgc gacggccgca gctggcgagt
     4201 ctgcgactgg gaacggatcg acaagggctt tagcgagacg gcgcggtggg ccctggaggg
     4261 ggccatcggc aactcggccg agatggccgc ctttcaccag gcgctggagg gagttcacaa
     4321 cgcccagacg agcatcgagg ccggcctggt ggcgctgggc tcgcgggacc tgctcgacgc
     4381 ccagtcgctc gacctgccgc cggtagtgaa cgatgtcgtg caattggagt tggcctgggc
     4441 ctgggaacgg tgcgcccgtt cggaccaggt gctggcggct tgcgatcgca tccgcgattc
     4501 gcgggccagt cccggagtgc attatctgcg ggcgctggcc cactggcggc gcgaggactt
     4561 tgccgccgtg ctggccgacg ctcagcagta ccgctcgatc gcgggcccgc atccacactt
     4621 gctcgaagtg gaggccgacg ctctggaggc tctgggacgg cgggcggagg cggcggaatg
     4681 ctggtggaag cggctggccc tgacccccga cgacgcggac gccctggccg aatactgccg
     4741 cctggcaggg gagtcagcgt caagggctgt cggcatcttg cgccgcgctg ctagcccgga
     4801 agaatttgcc cttggcgcgg ggcgaacggc catttatcgc gacgacgacg cgacgttggg
     4861 cgtcatcgaa cggtttttcc gcgagctaga cccgcaggcc gcggcgctcg agtcgctggc
     4921 ggcgtggcgg tgcgaacaac gccaagagca cccactggca gccgcccact atcgtctagc
     4981 agccgagcgc tccgcggacg aacaggaccg gcagcggcac ttcgcgcagt ttctggacgc
     5041 gatggcctac gccggccgcg cagctgcggg ctaccaggcc gccgacgatc cgcgagccgc
     5101 cttggcgcat ctcaccgcgg gactggaata tgacgaggcc cccatcgtca ttgacgatct
     5161 gccgcggctg atcgcgctgc acgcgcagcg agcgccccag gatccgcggc tgaagtacct
     5221 ggaagggctg gtggccatgg cggacggcca gttcgcggca gcggaagcca aattccggtc
     5281 agcggccaag gctccagcag ccgatccgcc ggaagggcac ctggcggaac ttgcccgcag
     5341 caagctggtc gaggcagtct acaggcagaa tcggctcgcg gcggcctacc aaacggcgac
     5401 cagcccgccc ggcgaagtct tccgagaatt ggcgctcctc tgccagcagg accgcaactg
     5461 ggccgcgctg gaggagctga tcgcgttgca ccgcacccgt gcgccggccg atccatggat
     5521 cgactacttc ctcgcgcggc gggcccaggc tcaggggaac gcggaggccg ggctcgccgc
     5581 cgtgcagcgg gcggaggccg caagcgacga gaccctgcgg ccgacgttgg gctggctcaa
     5641 gaacgagctg ctgatccaga gcggaggcat cggccaggcc tatcaaggca gtagcgacca
     5701 gcgggaggcg tttcgccggc tcgtcacgca cctggcgagt gaggaggact gggacggcgt
     5761 gctggagctg gccacgctgc acgcggcggc agcgccgcgg cggtccgcca cgctgtattg
     5821 gcgggcggca gcccattggc atcgcgggga gtaccaggaa ctggtggaca acctcagtcc
     5881 ctggcccgag gaccgcgtcg ggcgcctgga gccgggacaa ctcaccgagc tgtgcgacct
     5941 gctcgtgcgc agcctgctgc ggctgggcaa gctgcccgaa gcacaggcgg cggccgaccg
     6001 ggcccgcgac gaatacggcg tggagccgcc catggtcgcg ctcaagctcg cgcagggtga
     6061 caaggccgcc gtgctggaat tgatcgagca cccgcggatt tgccgggagc tgtttggccg
     6121 gcagctgtac cgcgaccgcg agctggcgcc gctgctatcc gacggcgaag tcgctgcgct
     6181 ccgccggcag caggccctgg cacttcccag cgaccactgg tcgcgccgca cggcgctcgt
     6241 cctgttcttc gccgagccgc tgccggccga acagctggac gagtgtgcgg caggagcaac
     6301 ggacgccgca attcggatca gcgggcggcc ggaccagaaa tcgttgctct ggaacctggg
     6361 atcggaaacg gtagtgctca ctgccgggag cgggccctat tgttcgtgcg gccaactgcc
     6421 cgagacgctc ggtgccgacg atccgcgacg cgcggcgctg gagcagcaca gcagctggat
     6481 cgcactcgat ttgttgccgg cggcccag
//
LOCUS       DASRJB010000118         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2574194, whole genome shotgun sequence.
ACCESSION   DASRJB010000118 DASRJB010000000
VERSION     DASRJB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574194"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VFB80_03415"
     CDS             complement(<1..1502)
                     /locus_tag="VFB80_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32834.1"
                     /translation="MPGFYPVPSTRTTGMLAQAQLLQQLHHDQLDIQRLTQQISTGYR
                     ISNPSEDAPAAQRGQTLQRMLELKAQARINLQTSQSYLDASDNTLGNVAATLNDVRAT
                     ALEAVSDTSTDAMRQTAAEQVANALEQLVATANQNFRGRYLFGGSRTNGPPFVYDGTH
                     VVYQGDEGLLESFVDVDLPYATNATGAEVFGAYSIEVRGTVDLNPALSADTPLAALFG
                     GRGVSLGSIVIGDGTSKSTIDLSSAATIGDVKELLEANPPTGRTITATITATGLTVDI
                     DDAGGGNLTIRDLPDGNTAQQLRILSPLGTGVTPVVGGDLNPRLQLTTPLASLQTGGP
                     PLDLASGVQVTNGGQTYTIDTSTAATVEDLLVAFNSSPANVLAQIDAGGDRLVIRSRL
                     SGTDFSIGENGGTTAAQLGIRSNTVDTPLTALNHGLGVSEKAGIDFSIVRKDGVQLDI
                     DIAGAATLGDVIDLINNHPANLVPGTQVVARLAPGGNGVELFDGNVGGAG"
     gene            complement(1527..3224)
                     /gene="flgK"
                     /locus_tag="VFB80_03420"
     CDS             complement(1527..3224)
                     /gene="flgK"
                     /locus_tag="VFB80_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZN32835.1"
                     /translation="MTLLGTIQNANNALIAAQLGLQVAGNNIANANTPGYIRQRLVQS
                     PAPLQKVGTLLLGLGVQVDGVQQVIDKFLAERLRNATSDLAAGEAQANTYAQLESLIN
                     ELGDNDLSTALTSFFGSLHDVLNQPESAAVRNVAVQEGRALAEQIRRLDQQVRTAHQD
                     VNQQIAGMANEINDLLAEIADLNLKIVSAEGGTVSPSDAVGLRDQREIALAKLAEITD
                     IRSIEQPTGDVTVFSGGEFLVNLGIHRTIEVVHSADDGLGIAELQIQDIGYPLTAGGG
                     KLSGLMTSRDTILRGFLDGLDQFTRTLTFEFNKVFSGGQGLSGYSSLTSEFGVSNAAL
                     ALDQAGLEFTPENGQFQIQVTNSQTGVTRTFDIRVDLNGVGTETTLNDLAAQLDAADG
                     ISASVTADGELQITADAPEITFAFAADTSGALAALGINTFFSGSGASTIGISQVLRSD
                     PKKLAMSSGGVAADTKNGLVLANLITEPLASRNGESLATLYDRLTNDVAQNAQTTRGA
                     TDGFRVFQQALEAQHQAISGVNLDEEAVRMIALQRVFQASARVIKTVDEMLEILVNL"
     gene            complement(3233..3736)
                     /gene="flgN"
                     /locus_tag="VFB80_03425"
     CDS             complement(3233..3736)
                     /gene="flgN"
                     /locus_tag="VFB80_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HZN32836.1"
                     /translation="MSTTAAAATDWEAEIGGLLAELSSVQSELLEVLSRKRDALAAAD
                     LEALAELQPQAEALAARLEHCQARRAELLEHACEAGLPGGSVSKLATRVSGGKSGKLG
                     QQVKGTAARLRLLQHHSLANWVLAQRSLLHVSQLLEIIATSGRMQPTYGDKELVHGCG
                     GLVNQQA"
     gene            complement(3790..4077)
                     /locus_tag="VFB80_03430"
     CDS             complement(3790..4077)
                     /locus_tag="VFB80_03430"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HZN32837.1"
                     /translation="MSSISSVTGLAGAGRAPAASAEFRGAFTDFVGQTFFGELLKQMR
                     ETVDKPKFLHGGMGEDIFQSQLDQILVERMTDASGPSFSGPMFDLLMARRS"
     gene            complement(4090..5166)
                     /locus_tag="VFB80_03435"
     CDS             complement(4090..5166)
                     /locus_tag="VFB80_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZN32838.1"
                     /translation="MQIKPRPLAVAILILFCVAAPALAYHRIGDVARIKGQEENVLHG
                     MGLVVGLKGTGDGDNKVMLRALAHYMELLGHRLASSPSGQQQLDELKNVKNVALVFVT
                     AIVPSGGAQQGDTLECLVSSAFSAKSLDGGTLMFTELYGPIPQDKTVYGIAKGLISID
                     DLGKPQSGRIPLGCQLEKTVRNEFVKNGKLLLVMNKDHATFQTTAEIESQINSQRDFT
                     PETGGQQVARAIDQVQIEVTIPPNYKDNPALFASLLLETSLAPPQVDTLVIINERKQA
                     LIIGEEVEIGPVAVMHKNRLIQIGDLQVNEAIGLNPLGDSSKTKLAALVDALNSLKVP
                     TADVIDIIKMLKHKRALFGELIIE"
BASE COUNT          819 a         1651 c         1837 g         1076 t
ORIGIN      
        1 tggcccgcgc cgccgacgtt gccgtcgaag agctcgacgc cgttgcctcc cggtgcaagt
       61 cttgccacga cttgagttcc gggcacgagg ttcgccgggt gattattgat caggtcgatg
      121 acgtcgccga gcgtcgccgc accggcgatg tcgatgtcga gctgcacgcc gtccttgcgg
      181 acgatgctga aatcgatgcc agccttttcg ctgacgccga ggccgtggtt cagggcggtc
      241 agcggagtat cgacggtgtt ggagcggatg cccagctggg ccgccgtcgt cccgccgttt
      301 tcgccgatcg agaagtccgt tccgctcagc cgcgagcgga tgacgaggcg atcgcctccg
      361 gcgtcgatct gggccaggac gttggccggc gagctgttga acgcgacgag cagatcttcg
      421 acggtggcgg ccgtgctggt gtcgatcgtg taggtctggc cgccattggt gacctgcacg
      481 cccgaggcaa gatccagcgg cgggccgccg gtctgcaagg aggctagcgg cgtcgtcaat
      541 tgcagccgcg ggttgaggtc gccgccgact acgggcgtga cgccggtgcc gagcgggctg
      601 agaatccgca gctgctgggc cgtgttgccg tccggcagat cgcgaatcgt caggttgccg
      661 ccgccggcgt cgtcgatatc gacggtcagg ccggtcgcgg tgatcgtggc cgtgatggtg
      721 cgtcccgtcg gcggattggc ctcaagcagc tccttcacat cgccgatcgt cgccgccgag
      781 ctgaggtcga tcgtgctttt gctcgtgccg tcgccgatca cgatgcttcc cagcgagacg
      841 ccgcggccgc caaacaatgc cgccagcggc gtatctgccg agagcgccgg attcaaatcg
      901 accgtgccgc ggacctcgat ggaataggcc ccgaagactt ccgcccccgt cgcgttggtt
      961 gcatagggca gatcgacatc gacaaagctc tccagcaggc cttcgtcgcc ttgatagacg
     1021 acgtgcgtgc cgtcgtagac gaacggcggg ccgttggtcc gcgagccgcc gaacaggtac
     1081 cgcccgcgaa agttctgatt ggccgtcgcg acgagctgct ccagggcgtt ggccacctgc
     1141 tccgccgccg tttgccgcat cgcgtcggtg ctggtatcgc tgacggcctc tagcgccgtc
     1201 gcccgcacgt cgttcagcgt cgcggcaacg ttgcccaggg tgttgtcgga ggcgtcgaga
     1261 tacgactgac tcgtctgcag gttgatccgg gcctgggcct tcagctccag catccgctgc
     1321 agcgtctggc cacgctgcgc ggccggggcg tcctcgctgg gattggagat ccgatagccg
     1381 gtgctgatct gctgcgtcag gcgctggatg tcgagctggt cgtggtgcag ctgctggagc
     1441 agctgggcct gggccagcat gccggtcgtg cgggtcgaag ggacgggata gaagccgggc
     1501 atgtcggttg cttgccgagg ggcggactac aggttgacca ggatctccag catttcatcg
     1561 accgttttga tcacgcgggc tgaggcctga aacacgcgct ggagggcgat catccgcacc
     1621 gcctcttcgt cgaggttgac cccgctgatc gcctggtgct gggcttccag ggcctgctga
     1681 aagacgcgga agccgtcggt cgcaccgcgc gtcgtctgag cgttctgggc gacgtcgtta
     1741 gtcagtcggt cgtagagcgt tgccagcgac tcgccgttcc gcgaggccag gggctcggtg
     1801 atcaagttgg ccagcacgag gccgtttttc gtatccgccg ccacgccgcc gctgctcatc
     1861 gccagcttct tcgggtcgct gcggaggacc tggctgatgc cgatggtgct ggcgccggag
     1921 ccgctgaaga acgtgttgat tcctagcgcc gccagcgcgc cgctggtgtc ggcggcgaag
     1981 gcgaacgtga tctcgggcgc gtcggcagtg atttgcagct cgccgtcggc ggtgaccgag
     2041 gcggagatgc cgtcggcggc gtcgagctgc gccgccaggt cattgagcgt cgtttccgtg
     2101 ccgacgccat tcagatcgac gcggatatcg aacgtgcgag tgacgccggt ctgcgaattc
     2161 gtcacctgaa tttgaaattg gccgttctcg ggtgtgaatt ccagcccggc ctggtcgagg
     2221 gccagcgcgg cattggaaac tccaaactcg ctcgtcagcg acgaatagcc ggagagtccc
     2281 tggccgccgg aaaagacctt gttgaattcg aacgtcagcg tccgcgtgaa ctggtccaga
     2341 ccatccagga agccgcggag gatcgtgtcg cgcgacgtca tcagccccga cagcttgccg
     2401 ccgcccgccg tgagcggata gccgatgtcc tggatttgca gctcggcgat tccgaggccg
     2461 tcgtcggccg agtggacgac ctcgatcgtc cggtggatgc ccaggttgac caggaactcg
     2521 ccgccgctga acacggtgac gtcgcccgtg ggctgctcga tcgaacggat gtcggtgatc
     2581 tcggccagct tcgcaagcgc gatctcccgc tgatcgcgca ggccgacggc gtcgctgggc
     2641 gaaacggtgc cgccttcggc cgaaacgatc ttcaggttca agtcggcgat ctcggccagg
     2701 aggtcgttga tctcgttcgc catgccggcg atctgctggt tcacgtcctg gtgggcggtt
     2761 cgcacctgct ggtccaggcg gcgaatctgc tcggccagcg cccgcccttc ctgcaccgcg
     2821 acgttgcgga ccgcggcact ttcgggctgg ttgagcacgt cgtgcaggct gccgaagaag
     2881 ctggtcaggg cggtgctgag gtcgttgtcg cccagctcgt tgatgagcga ttcgagctgg
     2941 gcgtaggtgt tggcctgtgc ttcgccggcg gccaggtcgc tggtggcgtt ccgcagccgc
     3001 tcggccagga acttgtcgat gacctgttga acgccatcga cctgcacccc caggcccagc
     3061 agcagcgtgc cgaccttttg cagcggagcg ggggactgga ccagccgctg gcggatgtag
     3121 cccggcgtgt tcgcgttggc gatgttgttg ccggcgacct gcaggccgag ctgcgccgcg
     3181 atcagggcgt tgttggcgtt ctggatggtg ccgagaagcg tcatcgcgtg ggtcaggcct
     3241 gctggttcac cagcccgccg cagccgtgga cgagctcctt atctccatat gtcggctgca
     3301 tccggccgct ggtggcgata atctccagca gctgcgagac gtgcaggagg gagcgctgtg
     3361 ctagcaccca gttggcgagg ctgtggtgct gcaaaagccg caagcgggcg gccgtgccct
     3421 tcacctgctg gcccagtttt cctgattttc cgccgctgac tcgcgtggcc agtttgctca
     3481 cgctgccgcc gggcaggcct gcttcgcagg cgtgctcgag gagctctgcc cggcgcgcct
     3541 ggcagtgctc caggcgagcg gcgagggctt cggcttgcgg ctgcaactca gccagcgcct
     3601 ccagatcagc cgcggccagc gcgtcgcgtt tgcgactgag gacttcgagc agctcgcttt
     3661 ggacgctcga cagctcggcc aagagcccgc cgatttcggc ttcccaatcg gtcgcggcgg
     3721 cagcggttgt tgacacagtg ggctcccggt caggagttca aggttcaaag ttcaaggtca
     3781 aagttcaggt caacttcggc gggccatgag cagatcgaac atgggtccac tgaacgacgg
     3841 gccgctggcg tcggtcattc gctcgacgag gatctggtcg agctgcgact gaaaaatgtc
     3901 ctcgcccatt ccgccgtgca ggaacttcgg cttatcgacc gtctcgcgca tctgcttgag
     3961 cagctcgccg aagaaggtct ggccgacgaa atcggtgaag gcgccgcgga attccgcgct
     4021 ggcagccggc gcgcggcccg cgccggccag gccggtgacg ctgctgatgc tggacatggg
     4081 cggtctccgt tactcgataa tcagctcgcc gaacagggcc cgcttgtgct tgagcatctt
     4141 gatgatgtcg atcacgtcgg ccgtcggcac cttgagcgag ttgagggcat cgaccagcgc
     4201 ggcgagcttg gtcttcgagc tgtcgccgag cgggttgagg ccgattgctt cgttgacttg
     4261 caggtcgccg atctggatca gccggttctt gtgcattacg gcgacgggac cgatctcgac
     4321 ttcctcgccg atgatgaggg cctgcttccg ctcgttgatg atcacgagcg tatcgacctg
     4381 cggcggagcg aggctggtct ccaggagcag cgaggcgaac agggccgggt tgtccttgta
     4441 gttcggcggg atcgtgactt cgatctgcac ctggtcgatc gcccgcgcga cttgctggcc
     4501 gccggtttcg ggagtgaagt cgcgctggct attgatctga ctttcgatct cggcggtcgt
     4561 ctggaacgtg gcatggtcct tgttcatgac caagagcagc ttgccgtttt tgacgaactc
     4621 gttgcggacg gttttttcca gttggcaacc aagcggaatg cgacccgact gcggcttgcc
     4681 gaggtcgtcg atcgaaatca accccttggc gattccgtag acggttttgt cctgcgggat
     4741 ggggccgtag agctcggtga acatcagcgt gccgccgtcg aggctcttgg cgctgaacgc
     4801 gctgctgacg aggcactcca gtgtgtcgcc ttgctgcgct ccgccggagg gcacaatcgc
     4861 ggtgacgaag acgagggcca cgtttttgac gttcttcagc tcgtccagct gctgctggcc
     4921 gctggggctc gaggcgaggc ggtggccgag caactccatg taatgggcca gggcccgcag
     4981 catcaccttg ttgtcgccgt cgccggtccc cttcagcccg acgaccaggc ccatgccgtg
     5041 cagcacgttc tcttcctgcc ccttgatccg cgccacgtcg ccgatgcggt gataggccag
     5101 cgcgggcgcg gccacgcaga acaaaatcag aatggctacg gcgaggggcc gtggtttgat
     5161 ttgcatgagt gcctcggtgc ggcttgattt cagatttcag atttcagatt tcaaatctgg
     5221 ggattgagcg gcttgatcct tcgtcaaatc tgaaatctga aatcctaaat ctgaaattct
     5281 taaaacggcg taaactggtc ccacagccgc gtcagccagc cgcgtttgta ggtgtcgcga
     5341 atctggccga agtcgcgctt atcgaccttg aggttggcga tgt
//
LOCUS       DASRJB010000119        10240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1162584, whole genome shotgun sequence.
ACCESSION   DASRJB010000119 DASRJB010000000
VERSION     DASRJB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162584"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="dprA"
                     /locus_tag="VFB80_03440"
     CDS             complement(<1..841)
                     /gene="dprA"
                     /locus_tag="VFB80_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZN32839.1"
                     /translation="MSALEPDGDNHAAELLLALTPGIGPRLRKSLLDHFGSAAAAVSA
                     SARELCDVPGIGRKLSENIVQERSRVDVRAELERCRASGVRIVAESGEGYPEVLRKIH
                     DPPGVLFVRGEILPSDGLAVAIVGTRHATQYGVTQGERLAAGLARAGYTIVSGLARGI
                     DAVAHRGALKAGGRTLAVLGSGVLNIYPPEHEQLAAEVIAKGALLSENPPDSPPLPGA
                     FPQRNRIVTGLALGVIVVEAADRSGALISARHAMEQGREVFAVPGRVDSRMSRGCHQL
                     IRDG"
     gene            complement(1106..1744)
                     /locus_tag="VFB80_03445"
     CDS             complement(1106..1744)
                     /locus_tag="VFB80_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN32840.1"
                     /translation="MGEPTAEPIIYVVDDDAAARQSVVALVSFKGLAARGFASAEEFL
                     SQFDPRQKGVLVTDVRMAGMSGLELQQALNERQASLPVVLITGYGDVPMAVRAMQAGA
                     VTFLEKPCHEQELWRGIMLALEREQTQHASRKLKAEIESRLESLSPDEREVFRRLLEG
                     QANKRIATDLDVGLRTIELRRSKIMKKMQAESLPDLVRMAILAGFLKAPASP"
     gene            complement(1962..3044)
                     /gene="aroB"
                     /locus_tag="VFB80_03450"
     CDS             complement(1962..3044)
                     /gene="aroB"
                     /locus_tag="VFB80_03450"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZN32841.1"
                     /translation="MVTTIRVALSERSYDIEIGRGLLSTASEFVRRPVAPSRAIVITD
                     SNVRSPHAERVAQSLAMGGIPVDLLSVPAGEASKSVAEAERLWNELVRLKADRKSVVV
                     AVGGGVVGDLAGFVAATFARGLSFVQVPTTLLAQVDSSVGGKVGINLPAAKNIVGAFW
                     QPAGVLIDLDVLATLPAREYRSGLAEVVKYGVILDASFFEYLENNVAALNARQPAVLE
                     QVVARSCRLKADVVQQDEREVSGLRAVLNYGHTFCHAIETVTGYGQFLHGEAVAIGMV
                     CASRLAVLLGRIDGSLTDRQAKLLAQLGLETSLPSLDHDALLAAMQHDKKTESGRLRF
                     VLPARLGLVELVSGVDPLLVRQALHR"
     gene            complement(3053..3277)
                     /locus_tag="VFB80_03455"
     CDS             complement(3053..3277)
                     /locus_tag="VFB80_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32842.1"
                     /translation="MADQFDPYREALVMEFNTIWPAEYENLSAAEKAKLEEKLHADAK
                     ACSQLEYIRTHTGFCRQITATPADVQRVGG"
     gene            3432..4226
                     /locus_tag="VFB80_03460"
     CDS             3432..4226
                     /locus_tag="VFB80_03460"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZN32843.1"
                     /translation="MQAYLDLLRHILDHGTPKSDRTGTGTRSIFGYQMRFDLAAGFPL
                     LTTKKLHLKSIAYELLWFLRGETNVQWLQERGVTIWDEWADEQGELGPVYGSQWRSWP
                     TPGGGRIDQISDVIDRIRSTPDSRRLIVSAWNVADIEQMALPPCHLLFQFYVAGGRLS
                     CQMYQRSADVFLGVPFNIASYALLTLLFAQVTGLAPGEFIHTFGDAHLYNNHVEQARL
                     QLSRQPRPLPKLSINPAVKSIFDFQYEDFRLTDYDPHPHIPAPVAV"
     gene            4223..4720
                     /locus_tag="VFB80_03465"
     CDS             4223..4720
                     /locus_tag="VFB80_03465"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZN32844.1"
                     /translation="MTRPSLIVAMTRRGVIGRGGTLPWRLSADLRRFKSLTMGHCLLM
                     GRKTFESLGRVLPGRTSIVLTRQPDYRPPPGVLVAASLDAALALTNQDAEPFVIGGGE
                     IFQLALPRAERLYVTWVEADVAGDTSFPAWDPTEWQLREQQRQGADDRNEFDTTFCIY
                     DRLPR"
     gene            4784..5026
                     /gene="yidD"
                     /locus_tag="VFB80_03470"
     CDS             4784..5026
                     /gene="yidD"
                     /locus_tag="VFB80_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZN32845.1"
                     /translation="MVEKPPPLILRLPALFLIGMVRCYQFTLSPIFGRQCRFQPTCSH
                     YMIGAVEKYGAWRGFWRGIWRILRCNPFCRGGYDPP"
     gene            5283..5618
                     /locus_tag="VFB80_03475"
     CDS             5283..5618
                     /locus_tag="VFB80_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HZN32846.1"
                     /translation="MAHCEYCDKKPAMGNQIETRGKAKYLGGVGTKVTGISRREFKPN
                     LQRVKITTPNGTTRYAKVCTRCLKKGLIRKAIKQAPFKLPVSAQKVAAAAAAAKPAAK
                     PAKAAKPAK"
     gene            5690..5980
                     /gene="gatC"
                     /locus_tag="VFB80_03480"
     CDS             5690..5980
                     /gene="gatC"
                     /locus_tag="VFB80_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZN32847.1"
                     /translation="MSLTRADVEKVSLLGRLRLSEAELDKMTEQLARVVGYVEQLREV
                     NTEGVQPMAHALDMHNVFRDDALAASLPRDAALANAPKRDSECYRVPAVLGE"
     gene            5987..7522
                     /locus_tag="VFB80_03485"
     CDS             5987..7522
                     /locus_tag="VFB80_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZN32848.1"
                     /translation="MTARSAAELIADLNARKVSSAELTKGYLDQIAKHDGRVKAFLLV
                     DPAAALARAKEIDDRRAAGKPVGKLGGLPVAIKDVICTQGVRTTCASRMLENFVPPYD
                     ATVVTKLKSADAVLIGKTNMDEFAMGGSTENSAYFKTTNPWDTSRVPGGSSGGAAACL
                     AAGMAPLSIGTDTGGSIRQPAALCSVTGLKPTYGRVSRYGLVAFASSLDQIGPLGRSA
                     EDCAVLLEVLAGHDPRDSTSANLPVPEYSKTVNQPLAGLKLGLVREYFGAGLDAEIEA
                     AVREAVKVYQSLGATVKEVSMPHSKYGIATYYIIAPSEASSNLARYDGVHYGYRTEEK
                     AMLAELAAEQQKLEAAKDQKKLADLDTPLVRLYRQTRAEGFGPEVKRRIMLGTYTLSA
                     GYYDAYYLKALKVRRLIRGDYDQAFEQVDFVVGPTTPSPAFKIGEKSSDPLAMYLEDL
                     YTVTANLAGIGGISIPCGFTKSGLPIGLQMQCRPFDEERLLRAAAMYQRATDWHTKRP
                     KLG"
     gene            7535..9013
                     /gene="gatB"
                     /locus_tag="VFB80_03490"
     CDS             7535..9013
                     /gene="gatB"
                     /locus_tag="VFB80_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZN32849.1"
                     /translation="MPDHTYDLIVGLETHVQLSTETKLFCGCSTEFGAQPNTQTCPVC
                     IGMPGSLPVMNREALQLALKTAIALNCEIPEFTKWDRKQYYYPDLPKGYQISQYDLPM
                     SQKGWLEIHDPKGRFQPKKVGIIRAHLEEDAGKSLHDEAAGKADSRIDLNRTGTPLLE
                     IVSAPDIRSTEETRAYLTELKLLLTYLGVSDCNMQEGSLRVDANINLHIHTPQGKVPT
                     PIVEIKNLNSFRFIERALAYEAKRQLEVWQQTGRKLGEAGTFKQTRGWDDAQGVTFAQ
                     REKEESSDYRYFPDPDLCPVVTTQAEVEQVRAGLAESPRAIRERLEKAHGITPYDSDV
                     IAAQGRPTVDYYEAVAKAAGDGKKAANWVTQEVLRVLNEQSWTLSQFPLTAASLADLV
                     KRVIAGEIEITRAKEVFNDMVANKRSAADSLQALGITKVDLSELEAICKELVAANPKI
                     VADVKGGKQQAVGALVGQAKKRNPNANPNDVRRICLELIEKM"
     gene            complement(9025..>10240)
                     /locus_tag="VFB80_03495"
     CDS             complement(9025..>10240)
                     /locus_tag="VFB80_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN32850.1"
                     /translation="QNSISRHNSPARGRGPLPLVAFSFALIVAPPLHAAEPNQLTPEQ
                     IADGWISLFDGETLFGWKAHSKADWAVKDGAIRVGSGERGLLCTSVQFDDYVLKADFR
                     AAKGTNSGIFLRTAPVVGMDDIKTKCYELNIAPPDNPFPTGSFVGRLKGKEVPERAGE
                     WQTFEATIDGGKCSVKLNGETVLEYTDPAPAHRGYIGLQLNTGEVEFRNIKLKPLGLE
                     SLFNGKDLAGWKNHPDSKSTFTVTPQAEIHVTSTGRGVLESEKRFGDFVVQLEAISHA
                     ANLNSGLFFRSIPGEMANGYESQIHNGFKNGDRTQPVDCGTGGIYRRVNARRVVPNDQ
                     QWFTKTIVADGPQIAVWVNGYQVTDWTDDRPPNDNPRSGLRTAAGTLQLQGHDPTTDL
                     SFRNIRAKELAK"
BASE COUNT         1881 a         3385 c         3257 g         1717 t
ORIGIN      
        1 ctccgtcgcg gatcagctgg tggcagccgc gggacatccg gctatccacg cgtcctggaa
       61 cggcaaacac ctcgcggcct tgctccatcg cgtgcctggc ggagatcagc gcgccggagc
      121 gatcggcggc ttccacgaca atgaccccca gcgcgagccc ggtgacgatg cgatttcgct
      181 gcggaaaggc gccaggcagc gggggggaat cgggcgggtt ctcgctcaac agcgcgccct
      241 tggcaatcac ttctgcggcg agctgctcgt gctcgggcgg atagatgttg agcacgccgc
      301 tccccagcac ggccagcgtc cggccgcctg ccttcagcgc gccgcgatga gcgacggcgt
      361 cgatgccgcg tgccaggccg ctgacgatcg tgtagcccgc gcgggccagg cccgccgcca
      421 ggcgttcgcc ctgggtcacg ccgtattgcg ttgcatgccg agtgcccaca atcgctaccg
      481 ccaggccgtc cgacggcaag atttcgccgc gaacgaacaa cactcccggc ggatcgtgta
      541 tcttccgcag gacttccgga tatccttcac ccgactccgc gacgatccgg acgccgctgg
      601 cgcggcagcg ctccagctcg gcgcgcacgt cgactcggct gcgttcctgg acgatgttct
      661 cgctcagctt gcggccgatt cccggtacgt cgcacagctc gcgcgcggag gcgctgacgg
      721 cggccgcggc cgagccaaag tgatccagca gcgatttccg cagccgcggg ccgatgccgg
      781 gagtcagggc gagcagcagc tcggctgcgt gattgtcgcc gtctggttcc agggcggaca
      841 tggcacaaag catagcgggc ccgtgtggcc gccgcacgcc gcgccccctg gaggatcagg
      901 aaatgcggcc ccagccatac cgcgggcaac ctgccattgc ctttgcggag ctggggccgc
      961 atctagccga gaaatcggcg gatggcggtg ggctcagcat acggaaggcc gcaatcggcc
     1021 ggcgattgat cgccggggtg cgcccgcgct cccgattgca gcggtgctgc ggaccaggcc
     1081 gagcgtcgct gtcagccact tggagctatg gactcgctgg ggcctttaag aacccagcca
     1141 gaatggccat tcgcaccagg tcgggcagcg actcggcctg cattttcttc atgatcttgg
     1201 agcgccgcag ctcaatggtc cgcaggccca cgtctaaatc ggtcgcgatc cgcttgttgg
     1261 cctgtccctc gagcagccgc cgaaacactt cccgctcatc cgggctcagc gactcgagcc
     1321 gggactcgat ctcggccttc aacttgcggc tggcgtgctg cgtctgctcc cgttccaagg
     1381 ccagcatgat tccccgccac aattcctgtt cgtggcaggg cttctccaga aaggtcacgg
     1441 ccccggcctg catcgcccgc acggccatcg gcacgtcgcc atagccggtg atcagcacga
     1501 cgggcaacga cgcctggcgt tcattcaagg cttgctgcag ctcgagtccc gacattccag
     1561 ccatgcgtac gtcggtaacc aatacgcctt tctggcgcgg gtcgaactgc gacaggaact
     1621 cctcggcgga agcaaagccg cgagccgcca gccccttgaa cgaaactaag gccaccaccg
     1681 actggcgcgc ggcggcatcg tcatcgacga cgtagataat tggttcagcg gtaggctcgc
     1741 ccacagaaag gcgtccagtt tctgtcttca ctgcattccc cgcggcggac cgtcgcccga
     1801 gcatacaaac ccgaagcgta agcgaggggc tttgtgaaga caccctggct cagaaagtcc
     1861 ctcgctgacg cttcgggtta ggaaaatcaa tcgccgctta tctattggat tcgccccact
     1921 ttgccaagtg ggccgccagt gcggcttgcc gcaaaatctg ctcaacgatg gagcgcctga
     1981 cggaccagca aaggatccac gccgcttacc agttcgacga gacccaagcg ggcgggcagc
     2041 acgaatcgca gcctcccgct ctcggtcttt ttgtcgtgct gcatcgccgc caatagagcg
     2101 tcgtgatcca agctcggcag cgatgtttcc aaccccaatt gcgcgagcag cttcgcctgc
     2161 cgatcggtga gcgacccgtc gattcgtccg agcagcaccg ccaggcgcga ggcgcagacc
     2221 atgccgatcg ccacggcttc cccgtgcaag aactggccgt acccggtgac cgtctcgatc
     2281 gcatggcaaa acgtgtggcc gtaattcagc acggcccgca agcccgacac ttcgcgctca
     2341 tcctgctgga cgacatccgc cttcagccgg caactccggg cgaccacctg ctcgagcacg
     2401 gccggctgcc gggcattgag cgccgcgaca ttgttctcca ggtactcgaa aaagctcgca
     2461 tcgaggatca ctccgtactt caccacttcg gccagtcccg agcgatattc ccgcgcgggc
     2521 aacgtggcga gcacgtccag gtcgatcagc acgcccgccg gctgccagaa cgcgccgacg
     2581 atattcttag ccgcgggcag gttgatgccg actttgccgc ccacgctgct atcgacctgg
     2641 gccagcagcg tggtcggaac ctgaacgaaa gagaggccgc gggcaaacgt cgccgccacg
     2701 aacccagcca gatcgccaac gacgccgccg ccgacggcca ccacgaccga cttgcgatcg
     2761 gcctttagcc ggaccaactc gttccacagc cgctcggcct cggccaccga cttgctcgct
     2821 tcgcccgcgg gaaccgacag caaatcgaca ggaattccgc ccatagcgag cgactgggcg
     2881 acgcgctcgg catgcggcga gcgcacgttg ctgtcggtga tgacgatcgc ccggctgggc
     2941 gccactggcc ggcgaacaaa ctcactcgcc gtagagagca ggccgcggcc gatctcgatg
     3001 tcgtagctgc gctcgctcag ggcgacgcgg atcgtcgtga ccatggcgta aatcagccgc
     3061 caacccgctg cacgtcggcc ggcgtggcag tgatctggcg gcaaaagccg gtgtgcgtgc
     3121 ggatgtattc gagctgcgag caagccttgg cgtcggcgtg cagcttctcc tccagcttcg
     3181 ccttctctgc ggcggagagg ttctcgtact ctgccggcca gatggtgttg aattccatca
     3241 ccagcgcttc gcggtaagga tcgaactggt cggccacgtc gggctccgga ggcaaacgga
     3301 taaactgggt tgtcggcatt gtagacagcg ttcctcggcg cgggaatgag tgaaagaggg
     3361 acggagcgac ggagagaatc gctcggtctc tccgtcctcc ctccctccgc cccgtctgct
     3421 acaacagcaa aatgcaagcc tacctcgacc tgctgcggca catcctcgac cacgggacgc
     3481 ccaagagcga ccgcacggga acgggcacgc gcagcatctt cggctaccag atgcggtttg
     3541 acctggcggc cgggtttcca ctgctgacga ccaaaaagct gcacctgaag tcgatcgcct
     3601 acgagctgct ctggttcctc cgcggcgaga cgaacgtgca gtggctccag gagcgcggcg
     3661 tcaccatctg ggacgaatgg gccgacgagc aaggcgaact cggcccggtc tacggcagcc
     3721 aatggcgctc gtggcccacg cccggcggcg gtcgcatcga ccagatcagc gacgtcatcg
     3781 acaggattcg cagcacgccc gactcccgcc ggctgatcgt cagcgcctgg aacgtcgccg
     3841 acatcgagca gatggccttg ccgccgtgcc acctgctgtt tcagttctac gtggccggtg
     3901 ggcggctctc gtgccagatg taccagcgga gcgccgacgt ctttctgggg gtgccgttca
     3961 acatcgcgtc gtacgccctg ctgacgctgc tcttcgccca ggtgaccggc ctcgcgccgg
     4021 gcgagttcat ccacacgttc ggcgacgccc acctctacaa caaccacgtc gagcaggccc
     4081 ggttgcagct ctcgcgccag ccgcggccgc tgcccaagct gtcgatcaat cccgccgtga
     4141 agtcgatctt cgacttccag tacgaggatt ttcggctgac cgattacgac ccgcatccgc
     4201 acattccagc gccggtggcg gtgtgacgcg acccagcctc atcgtcgcca tgacccgccg
     4261 cggcgtgatc ggccgcggcg gaacgctgcc ctggaggctg tcggccgatc tgcggcggtt
     4321 caagtcgttg acgatgggcc actgtctgct gatgggccgc aagacattcg agtcgctcgg
     4381 ccgcgtgctg ccgggacgca cgtcgattgt cctcacgcgc cagccggatt atcgcccgcc
     4441 gcccggggtg ctcgtggccg cgagcttgga cgcagcgctg gccctgacga atcaagacgc
     4501 cgaaccgttc gtgatcggcg gtggcgagat ctttcaactc gccctccccc gcgccgagcg
     4561 gctgtatgtc acctgggtgg aggccgatgt ggccggcgac acgagctttc cggcctggga
     4621 tcccaccgag tggcaactgc gcgagcagca gcggcagggg gccgatgacc ggaacgagtt
     4681 cgacacgacc ttctgcatct acgatcgcct gccgcgatga tgactgcgtt gtgctattga
     4741 acactttcgc aaataacata caatatcggg tgaagaacga gcgatggtcg agaaaccgcc
     4801 cccgctgatc ctccgcttgc ccgctttgtt cctcatcggg atggtgcgat gctaccagtt
     4861 caccctcagc ccgatattcg gacggcaatg ccgctttcaa ccgacctgca gccattacat
     4921 gatcggggcg gtcgagaaat acggggcgtg gcgcgggttc tggagaggca tttggcgcat
     4981 cctgcggtgc aacccgttct gccggggcgg ctacgacccg ccgtaacatg catcgcgggg
     5041 cttaacgtgc ataaaatgca taatcgcatg ccatccgttg agctacaaat ccttgtggat
     5101 aaatgactta cgacaaaatg catatccaga aaaatggtgc attatgcatg catcgtggcc
     5161 gccgaggcgg agcagcggcc gcgttggcgc ttgtggggtg actgcttata ttgtgtgatt
     5221 ccctctccgc cagaagagcc ggcgtgtggg cgcaagtccc gccggcgcaa ggaaatcgag
     5281 cgatggcaca ctgcgagtac tgcgacaaaa agcccgccat gggcaaccag atcgaaaccc
     5341 gcggcaaggc gaaatacctg ggcggcgtcg gtaccaaggt caccggcatt tcccgtcgcg
     5401 aattcaagcc gaacctccag cgggtcaaga tcacgactcc caacggcacc acgcggtatg
     5461 ccaaagtttg cacgcgctgc ctgaagaagg gcttgatccg caaagcgatc aagcaggcgc
     5521 ccttcaagct gccggtctca gcccaaaagg tcgccgccgc ggccgcggct gccaagcccg
     5581 ccgccaaacc ggccaaggcc gccaagccgg cgaagtaacg tgggcgggcc agtgcccgcc
     5641 ctacgaacct ctccgcgccg ctgctcgtcc actggaagga ggttcgcaca tgtcgctaac
     5701 ccgggccgat gtcgagaagg tatcgctgct gggccggctg cggctctcgg aggccgagct
     5761 cgacaagatg accgagcagc tggcgcgcgt cgtcggctac gtcgagcagc tccgcgaggt
     5821 gaacaccgag ggcgtgcagc cgatggcgca tgccctcgac atgcacaacg ttttccgcga
     5881 cgacgccctc gccgctagcc tgccgcgcga cgcggcgctg gccaacgcgc ccaagcgcga
     5941 cagcgagtgc tatcgagtgc cggccgtgct gggagagtaa ccgccaatga ctgcccgctc
     6001 cgccgccgag ctgatcgccg acctgaacgc gcgcaaggtt tcgtctgccg aattgacgaa
     6061 gggttatctc gatcagatcg cgaagcacga cggcagagtc aaggcattct tgctggtcga
     6121 tcccgcggct gcattggcgc gggccaaaga aatcgacgac cgccgcgcgg ccggcaagcc
     6181 agtgggaaaa ctgggcggtt tgcccgtggc gatcaaggat gtgatctgta cgcaaggcgt
     6241 gcggacaacc tgcgccagtc ggatgctaga gaacttcgtg ccgccctatg acgcgacggt
     6301 ggtgacgaag ctcaagtccg ccgatgccgt gctgatcggc aagacgaaca tggatgagtt
     6361 cgcgatgggt ggctcgaccg agaacagcgc gtacttcaag acgaccaacc cctgggacac
     6421 gtcgcgcgtg cccggcggct ccagcggcgg tgcggcggcc tgtctggcgg ccggcatggc
     6481 gccgctctcg atcggcaccg acacgggcgg ctcgatccgg cagccggcgg cgctgtgcag
     6541 cgtgacgggc ctcaagccga cctatggccg tgtcagccgc tatgggctcg tcgccttcgc
     6601 cagctcgctc gaccagatcg ggccgctggg gcgctcggcc gaagactgcg ccgtgctgct
     6661 ggaagtgctg gccggccacg atccgcgcga ttcgacgagc gccaacctgc ccgtgcctga
     6721 atactcgaag acggtgaacc agccgctggc ggggctgaag ctgggcctcg tgcgcgagta
     6781 tttcggcgcg ggcctggacg ccgagatcga ggccgccgtg cgcgaggcgg tgaaggtcta
     6841 tcagtcgctc ggcgctaccg taaaagaagt ctcgatgccc cacagtaaat acggcatcgc
     6901 aacgtattac atcatcgccc ccagcgaggc ctccagcaat ctggcccgct acgatggcgt
     6961 gcactacggc taccgcaccg aggagaaagc gatgctcgcg gagctggccg ccgagcagca
     7021 gaagctcgaa gccgcgaagg accagaagaa gctcgccgat ctcgacacgc cgctggtgcg
     7081 gctctatcgc cagacgcggg ccgaaggttt tggccccgag gtcaagcggc ggatcatgct
     7141 cggcacctac acgctgagcg ccggctacta cgacgcctac tatctcaagg cgctcaaggt
     7201 ccgccggctg atccgcggcg actacgacca ggccttcgag caggtcgatt tcgtcgtcgg
     7261 ccccacgacg ccctcgccgg cgttcaagat cggcgaaaag tcgagcgacc cgctggcgat
     7321 gtacctggag gacctgtaca ccgtcaccgc caacctcgcc ggcatcggcg gcatcagcat
     7381 cccctgcggc ttcaccaaat ccgggctgcc gattggcctg cagatgcagt gccgcccctt
     7441 cgacgaagag cgcctgctgc gagccgcggc catgtatcag cgggcgaccg attggcacac
     7501 gaagcggccc aaactcggct gacaacgact ttacatgccc gaccacacct acgacctgat
     7561 cgtcggcctg gaaacgcatg tccagctctc gacggaaacg aaactgttct gcgggtgcag
     7621 cacggagttc ggcgcgcagc cgaatacgca gacgtgcccg gtttgtatcg ggatgccggg
     7681 gagcctgccg gtgatgaacc gcgaggcgct tcaactggcg ctcaaaacgg cgatcgcgct
     7741 gaactgcgag attcccgagt tcaccaagtg ggaccgcaag cagtattact atcccgactt
     7801 gcccaagggc tatcagatca gccagtacga cctgccgatg tcgcagaagg gttggctcga
     7861 gattcacgat cccaagggcc gctttcagcc caagaaggtc ggcatcatcc gcgcccactt
     7921 ggaagaagat gcgggcaaga gcctgcacga cgaagcggcg ggcaaggccg actcgcgaat
     7981 cgacctcaac cgcacgggca cgccgctcct ggaaatcgtc agcgccccgg acattcgcag
     8041 caccgaagag acgcgggcct acctcacaga gctgaagctg ctgctcactt acctgggcgt
     8101 ctcggactgc aatatgcagg agggctcgct gcgggtcgat gccaacatca acctccatat
     8161 ccacactccg cagggcaaag tccccacgcc gatcgtcgag atcaagaacc tcaacagctt
     8221 ccggttcatc gagcgggccc tggcctacga ggcgaagcgg cagctcgaag tctggcagca
     8281 gaccggcaga aagctgggcg aggcgggcac gttcaagcag acccgcggct gggacgacgc
     8341 gcagggcgtc acgttcgccc agcgcgagaa agaagaatcg agtgactacc gctattttcc
     8401 cgatccggac ctctgcccgg tcgtgacgac gcaggcggaa gtggaacagg tccgcgccgg
     8461 cctggccgag tcgccgcggg cgattcgcga gcgactggaa aaggcgcacg gcatcacgcc
     8521 ctacgacagc gatgtcatcg ccgcgcaggg caggccgacg gtcgattact acgaggccgt
     8581 cgccaaagca gccggtgacg gcaagaaggc cgccaactgg gtcacgcagg aagtgctccg
     8641 cgtgctcaac gagcagagct ggacgctgtc ccagttcccg ctgacggctg cgagtctcgc
     8701 cgacctggtg aagcgggtca tcgccggtga gatcgagatc acgcgggcca aggaagtctt
     8761 caacgacatg gtggccaaca agcgatcggc cgccgactcg ctgcaagccc tgggcatcac
     8821 gaaagtcgat ctttcggagc tggaggcgat ctgcaaggaa ttggtcgcgg ccaatccgaa
     8881 aatcgtcgcc gacgtcaagg gcggcaagca gcaagccgtc ggcgcgctcg tcgggcaggc
     8941 caagaagcgc aaccccaacg ccaaccccaa cgacgtgcgg cggatttgcc tggagctgat
     9001 cgagaagatg tagttagatc gggcttattt cgccagctcc ttcgcccgga tgttccggaa
     9061 cgacaggtcg gtcgtcgggt cgtgcccttg tagctgcagc gtgccggcgg cggttcgcag
     9121 gccgcttctg gggttgtcgt tcggcgggcg gtcgtcggtc cagtcggtga cctgatagcc
     9181 gttgacccag acggcgatct gcggcccgtc ggcgacgatg gtcttggtga accactgctg
     9241 gtcgttgggc actacgcggc gcgcgttcac gcggcgatag atgccgccag tcccgcaatc
     9301 gaccggctgg gtacggtcgc cgtttttgaa gccgttgtgg atctggctct catagccgtt
     9361 ggccatttcg cccggaatgc tgcggaagaa gagtccgctg ttgaggttgg ccgcatgact
     9421 gatcgcctcg agctgcacga cgaagtcgcc gaaccgcttt tccgactcca gcacgccgcg
     9481 gccggtgctg gtgacgtgga tctcggcttg cggcgtgacc gtgaacgtgc tcttgctgtc
     9541 gggatggttc ttccagccgg ccaggtcctt gccgttgaac agcgattcca ggcccagcgg
     9601 cttgagcttg atgttccgga actcgacttc gccggtattg agctggaggc cgatatagcc
     9661 gcggtgcgcc ggcgcggggt cggtgtattc gagcaccgtt tcgccgttga gcttgacgct
     9721 gcacttgccg ccgtcgatcg tggcctcgaa cgtttgccac tcgccggccc gctcgggcac
     9781 ttccttgcct ttcagacggc cgacgaagct gcccgtgggg aacggattgt ccggcggagc
     9841 gatgttcagc tcataacact tcgtcttgat gtcgtccatg ccgacgaccg gcgcggtccg
     9901 cagaaagatg ccgctgttgg ttcccttcgc cgcgcggaag tcggccttca gaacgtagtc
     9961 gtcaaattgc acgctggtgc agagcaggcc gcgctcgccc gagccgacgc ggatcgcgcc
    10021 gtccttcacg gcccagtcgg ccttgctgtg ggccttccag ccgaagagcg tttcgccgtc
    10081 gaacaggctg atccaaccgt cggcgatttg ctccggagtg agttggttcg gctcggcggc
    10141 atggaggggc ggggcgacga taagcgcgaa cgagaaagcc acgagcggaa gcggccctct
    10201 cccccgcgcg ggggaattat gtcgcgaaat cgaattttgg
//
LOCUS       DASRJB010000120         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3984887, whole genome shotgun sequence.
ACCESSION   DASRJB010000120 DASRJB010000000
VERSION     DASRJB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984887"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFB80_03500"
     CDS             <1..458
                     /locus_tag="VFB80_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN32851.1"
                     /translation="SVPSAELGTPFNEEVEHLRRLVDIATLHGVRVGLKSQLGRLSQD
                     PDTVMVLCDNVKGLGLTLDPSVYIFGPYQGRSIDKVMKYVYHLQLRDTNKKAFQVRVG
                     QGDIEYGRLISQLQKLHYHRALSVNIKEQPDVDHVGELRKLRLLLESLL"
     gene            560..2353
                     /locus_tag="VFB80_03505"
     CDS             560..2353
                     /locus_tag="VFB80_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32852.1"
                     /translation="MTRKLTLGLVLSLGSLLLAGCSKSPPPTAPTAAALPPAPPPPPV
                     LTPAAPLVPPPAVAPASPAPPDTPVPASETPPAPAASAPPAAAPRAPQARSGRTKSKS
                     GNGASEGQPLGPGALEALAGRAKHPLANAQVGDWADFRASMPDLGLGGLARAQGDIEM
                     RMKVASRTDTTVTLSREGTAPGGRPLRTGGDITYDLTQPWDPTKAALMREPGEENQVK
                     LVGRTAETVRISDFVMPTEKFRYQITAGGGDLKIESEVSAWFAAEAPLTGMVAMEMDL
                     LGFKVKMQLTSWGPFHEGTLREVPGAKLTKFDHSRVTFAVGQQSFEMMWLGFGQGPSV
                     IDQAGQEHFGSNAIRYLAPGNVFRVRTGLNSNAEMLGSLVLEQGGPASLAQLVAHLPR
                     DNRNFEFSPPIQPAVWERTLARAKVGDWVVWKEYENGRSKYREVTGVSPNEVTGWEVY
                     NLEKSFSHALRYKLDEPKPPKAKNTAAATPPATKKTTAKSTSKKPSTTKKPSTSKKPA
                     PKKPDDDDPPEPKPEAAEPPPPQPVVVGTETVTVMGRPLTCEVVEVPHDSGRGVALKL
                     WISAEVPLDGVVRREEKGKLVYLLEEFGRAK"
     gene            2428..3213
                     /locus_tag="VFB80_03510"
     CDS             2428..3213
                     /locus_tag="VFB80_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZN32853.1"
                     /translation="MLRFDLVDAFTAEPFAGNPAAVYLLEDWQPNAWLQRVAREMNQS
                     ETAYLVRTSDGFELRWFTPLVEVDLCGHATLASAHALWQRGIAKGDQIAFSTRSGTLT
                     ATRAGDEIELDFPLLVEQSASPPPGLSEALGITPLYIGQSRHDLVVELGNEEQVRQLR
                     PDFSRLAKVATRGVIVTARSSDPAFDFVSRFFAPAAGIDEDPATGSAHCCLADFWQKR
                     LHKTSFRAFQASPRGGVLSVRVQGQRVILSGRAITVARGELLA"
     gene            3291..>3548
                     /locus_tag="VFB80_03515"
     CDS             3291..>3548
                     /locus_tag="VFB80_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32854.1"
                     /translation="MRRTNRYLLSALLLLATAATAHAQPTGYIISFAHYVDGISYGSG
                     FYPGQALSVDQDSSVYNQFYRVYFEAVAGVYRSRPYSGVHTI"
BASE COUNT          639 a         1216 c         1122 g          571 t
ORIGIN      
        1 tcagcgtccc ctcggccgag ctgggcacgc cgttcaacga agaggtcgag cacctccgcc
       61 ggctggtcga tatcgccacg ctgcacggcg tgcgggtcgg cctgaagagc cagctcggcc
      121 ggctgtcgca ggaccccgac acggtgatgg tcctctgcga caacgtgaag ggcctgggcc
      181 tgacgctcga tcccagcgtc tatatttttg gaccctacca gggccgcagc atcgacaagg
      241 tgatgaagta cgtctatcac ctgcagctcc gcgacacgaa caagaaggcc ttccaggtcc
      301 gcgtcggcca gggcgacatc gagtacggcc gcctcatctc gcagctgcag aagctgcact
      361 accaccgcgc tctctcggtc aatatcaagg aacagcccga cgtcgatcac gtcggcgagc
      421 tccgtaagct gcggctgctg ctggagagcc tgctctaagt atttcaaaat gagagaaccg
      481 gccctccggg cctgattgaa ccgcgcgttt gcattcatgt ttgcgacgac tacaatcggc
      541 gcgaactgcg aggtgctgca tgacgcgcaa gctcacactg ggcctggttc tttcattggg
      601 gtcgctgctg ctcgcgggtt gttccaagtc gccgcccccc accgcgccga ctgcggcggc
      661 cttgcctccc gctccgccac cgccgcctgt cttaacaccc gcagctccac tcgttccgcc
      721 gccggccgta gcacccgcgt cgccggctcc gcccgatacg ccggtgccgg cgtccgaaac
      781 gccgccggcg ccggccgcaa gtgctccgcc ggccgcggcg ccgcgagcgc cgcaagcccg
      841 ttcgggcagg accaagtcca agtccggcaa cggggcttcc gagggccagc cgctggggcc
      901 cggagcgctg gaggccctgg ccgggcgggc gaagcacccg ctggccaatg cgcaggtggg
      961 cgactgggcc gatttcaggg ccagcatgcc cgatctgggc ttgggcggcc tggcccgcgc
     1021 tcaaggcgac atcgagatga gaatgaaggt cgcgtcgcgg accgacacga ccgtgacgct
     1081 gtcgcgcgaa ggaaccgcgc cgggcggccg cccgctgcgc acgggcggcg acatcaccta
     1141 tgacctgacg cagccttggg atcccacgaa ggccgccctg atgcgggagc cgggcgaaga
     1201 aaaccaggtg aagctcgtcg gccgcaccgc ggagacggtg cgaatctcgg atttcgtaat
     1261 gcccaccgaa aagtttcggt accagatcac cgccggaggc ggcgatctga agatcgagtc
     1321 ggaagtttcg gcctggttcg cggccgaagc gccgctcacg ggtatggtgg cgatggaaat
     1381 ggacctgctg ggcttcaagg tcaagatgca gctgaccagc tgggggccgt tccacgaggg
     1441 gacgctccgc gaggtgcccg gagcgaagct cacgaagttc gaccactcgc gagtcacgtt
     1501 cgcggtcggc cagcagagct tcgagatgat gtggctcggc ttcggccaag ggcccagcgt
     1561 catcgaccag gccggccagg agcacttcgg ctccaatgcc atccgctacc tggcccccgg
     1621 caacgtgttt cgcgtccgga ccggactgaa ctccaacgcc gagatgctgg gcagcctggt
     1681 gctggagcaa ggcggtcccg cctccctcgc gcagctcgtg gcccacttgc cgcgcgataa
     1741 tcgcaacttc gaattctcgc cgccgatcca gccggctgtc tgggagcgga cgctggcccg
     1801 tgcgaaggtc ggcgactggg tcgtctggaa ggaatatgag aatggccggt ccaagtaccg
     1861 cgaggtgacc ggcgtcagtc cgaacgaagt cacgggctgg gaggtctaca acctggaaaa
     1921 gtcgttctcg cacgccctgc gctacaagct cgacgaaccc aagcccccca aggcaaagaa
     1981 tacggccgcc gcgactccac ccgcgaccaa aaagacgacg gccaagtcca ccagcaagaa
     2041 gccgtcaacc acgaagaaac cgagcacatc aaagaagccc gcccccaaaa aacccgacga
     2101 cgatgacccg ccggaaccga aacccgaggc cgccgagccg ccgccacccc agccggtcgt
     2161 cgtcggcacc gaaacggtga cggtgatggg ccgcccgctg acgtgcgagg tcgtggaagt
     2221 cccccacgac agcggccgcg gagtggcgct gaagctctgg atttcagccg aggttcccct
     2281 tgacggcgtc gtccgccgcg aggaaaaggg gaagctcgtc tacctgctcg aagaattcgg
     2341 ccgcgcgaag taggtgggcg aacccgtgcc cgcggcaggt gtccagagta gacctcacgc
     2401 tccgcgtgag gccgccggag cctccccatg ctacgcttcg acctcgtcga tgccttcacc
     2461 gcagagccct tcgcgggcaa ccccgcggct gtctatctcc tggaggactg gcagcccaat
     2521 gcctggctcc agcgggtggc ccgcgagatg aaccagtcgg agacggccta tctcgtgcgg
     2581 accagcgacg gctttgagct ccgctggttc acgccgctgg tcgaggtcga tctgtgcggc
     2641 cacgcgacgc tggcctcggc ccatgcactc tggcagcgcg gcatcgccaa aggcgatcag
     2701 attgcctttt cgacgcgcag cggcacgctc acggccacgc gcgcaggcga cgaaatcgag
     2761 ctcgattttc cgctattggt cgagcaatca gccagcccgc cgccggggct gagcgaggcc
     2821 ctaggcatta cgccgctcta tatcggccag agccggcacg acctggtcgt ggagctcggc
     2881 aacgaagagc aagtccgcca gctgcggccc gacttttcgc ggctggcgaa agttgccacg
     2941 cggggcgtga tcgtcacggc ccgctccagc gacccggcct tcgatttcgt ctcgcggttc
     3001 ttcgctcccg ccgcgggaat cgacgaagac cccgccacgg gctcggccca ctgttgcctg
     3061 gccgacttct ggcagaagcg gctgcacaag acgtcgttcc gcgcctttca agcctcgccc
     3121 cgcggcggcg tgctctcggt ccgcgtccag ggccagcggg tgatcctgag tggccgcgcc
     3181 atcaccgtcg cccgcggcga gctattggcg tagagcaagc gtccccgctt gctcagggtg
     3241 aacaagcttc tacggggcga gtcttgcttt cgcgctgcga aacggcgaca atgcgacgca
     3301 ccaaccgcta tctactctcc gcgctgctac tgctcgcaac cgcagcgacc gcccacgccc
     3361 agccgaccgg ctacattatt tccttcgcgc attatgtgga cggcatttca tacggctcag
     3421 gattctatcc cggccaggca ctttctgtag atcaagactc cagcgtttac aaccaatttt
     3481 atcgggtgta ttttgaggcc gttgccggtg tttataggtc acgaccgtac agcggagtgc
     3541 atactatt
//
LOCUS       DASRJB010000121         7276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2858101, whole genome shotgun sequence.
ACCESSION   DASRJB010000121 DASRJB010000000
VERSION     DASRJB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858101"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFB80_03520"
     CDS             complement(<1..1015)
                     /locus_tag="VFB80_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN32855.1"
                     /translation="MSAAFVRDSAPRRHSASSQRYSARQLFLEPLEGRCLLTAGMIGG
                     AVFEDIPGDGITPDDLPLADWGVQLYADDGDGVFDAADPLVAEQTTDGGGGYAFGGLP
                     AGKYFVQQQLPLAWIQAEEVEPHEIEVIPPDASLPLVAERNDTISMATATGLSSATPG
                     TYIARGTIGDNKRIDPLLDVDMFQVQLNAGDRLRIDVDTWEFGSELASMLRLFDASGA
                     QLLGHDGGGPAGPFLEFVAKADGLYYAGLASFANGLYDPFVEGSGDIVEDTGDYTIEI
                     EVGPRPGAEPVAVTLGVDEQLGGIDLAAWHLGSIVGQMYDDTNGNGIQDPGELGLDNE
                     VLLV"
     gene            complement(1086..1415)
                     /locus_tag="VFB80_03525"
     CDS             complement(1086..1415)
                     /locus_tag="VFB80_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32856.1"
                     /translation="MLAGGSVNLLQDGSAGTERMDMACASSVSGSLEIRQLGSERADQ
                     VSADFQFFGTGSITVHINLLGGDDLLEASLLLLGKFPVFFALADMGDGLDIARVSPAI
                     EVRNGEE"
     gene            1737..2318
                     /locus_tag="VFB80_03530"
     CDS             1737..2318
                     /locus_tag="VFB80_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32857.1"
                     /translation="MTAPFADLLERARRGDSNALAALASQYEPEVRVMARVLLGPALR
                     PYLDSLDLVQSVHRSLLAGLRHGKIDLTNPQRLVALALTMVRRKIARQWRRHQRQERP
                     VGGDAETLADRLATLCSAEDDPARRAERNDSLRLVCRQLTETERRLIELRLNGCSTIE
                     AAGQLGLDADVLRVRLSRLRQRLQQAGILEEFV"
     gene            complement(2315..3262)
                     /locus_tag="VFB80_03535"
     CDS             complement(2315..3262)
                     /locus_tag="VFB80_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZN32858.1"
                     /translation="MPRKVIIDCDPGIDDAVALALALFDPRLEVVAVTAVAGNIPADR
                     ATQNVQAIVERLDPPRYPRLGAAAPPDDAPYADGRHLHGPDGLGGAGLAVSQLARQHP
                     SEKLMADECRIAPGDVTIVCLGPLTNVARALTRDPDFVNQAGRLVIAGGSVRCVGNAT
                     PCAEFNIHADPISARAVFKSPLTKTLIPLDVTDQVVWSFDFLDKLPDETTRAGKLLRL
                     ALPYLFRTHRQHLGLESIRLPDAVAMAAVLHPELFRMEELAGDVETNGEITMGATIFD
                     RRPNCPHRADIEVALEIDPAAVADAILRGLAEAGRQSAS"
     gene            3361..4371
                     /gene="trpD"
                     /locus_tag="VFB80_03540"
     CDS             3361..4371
                     /gene="trpD"
                     /locus_tag="VFB80_03540"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZN32859.1"
                     /translation="MITSQLGRIAAGVDLTQDEMTEVIDQLLTGAVPQNEIAVLLTAL
                     RAKGETADEIAGAARAMRRHMTPIRTSRTGLVDTCGTGGIGSDLFNISTAAAIVAAAA
                     GVPIAKHGNRSVTSKSGSADVLRELGVNVDAPVPVVERCLDELGICFCFAPQLHPAMK
                     HVAPVRKQLGFATIFNLLGPLCNPAGATRQLLGVGRRELHRTMADVTARLGTDHTLVV
                     HGTDGIGEVSLSAPTEVIAVSREGQQSIAWRPEDFGLTTAGRESLRVSSPQESAALIR
                     RIFSGEPGPPHDVVVINAAAALLAAEAQREARRAAEQAAAAIDSGAAARLLSAWAEMS
                     FD"
     gene            complement(4368..5447)
                     /locus_tag="VFB80_03545"
     CDS             complement(4368..5447)
                     /locus_tag="VFB80_03545"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN32860.1"
                     /translation="MRVLTAALLTIALLLCTADAGHAQPARPGTFTEAAQAGIDYVLQ
                     GEYAGSLFIPGYGFEPAGLQVVALGGGKFDAVCYRGGLPGVGWDRATKAGCSGEAVDG
                     RLTLSGPAGEFVVDGTRALSFDAAGRLLGRLGKVERSSPTMGLAPPPGAIVLFGGGST
                     EQLQGAKVTPDGLLLAGVLTKMPVSAFHLHLEFRTPFMPAARGQARGNSGVYIQQRYE
                     LQILDSFGLEGVENECGGLYRQTRPDVNMCLPPLAWQTYDIWFTPPVFAAGGQTKLAD
                     ARLTALHNGVPIHWHRAITAKTGGGKVEGPEPFPINLQDHGNPVAFRNIWIVPGEGDQ
                     QLTGAQAGPGYTRAQRVRLRWLQAR"
     gene            complement(5603..6532)
                     /locus_tag="VFB80_03550"
     CDS             complement(5603..6532)
                     /locus_tag="VFB80_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN32861.1"
                     /translation="MIHRSFAVAAAGWLLAVAAASAADPIPGIGPAGEVKKLHTGLQF
                     TEGPASDGQGNLYFSDVAGNKLHKVDATGELTTVLDPSKHTNGLMVNAAGNIVACEMD
                     GRLIEVNPKTKEVKALAEGYEGKRFNAPNDLVIDKEGGVYFTDPHFRAPMPLPQGVRA
                     FYYRAADGKVTRLGVIENAPNGVKLSPDEKTLYVIPSLQAEMLAYPVEGLGKLGAQRT
                     FCTLKQAPGKTAGGGDGMTIDSKGNLYIATALGLQVFSPDGKALGIIELPEQPANCTF
                     GGRDNKTLYVTARTSLYAVPMTATGHQYPAGKR"
     gene            complement(6604..>7276)
                     /locus_tag="VFB80_03555"
     CDS             complement(6604..>7276)
                     /locus_tag="VFB80_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 10"
                     /protein_id="HZN32862.1"
                     /translation="EEVVKRYRGKVNLWHVAAGMNLDGALEFSEEQRLRLVVEAVDRV
                     RAVDPRTPLVMSFAQPWGEYIVRKDQELTPLHFADTLVRGELGLAGVGLEIHYGYWPG
                     GTLPRDPLEIARQIDRWSQIGIPLMVFLSAPSSAVADPQARHPGRPLIDLRPGGPTTA
                     WQHVLLQWVLPLLLAKQSVQAIIWDAWQDNQPHEMAHSGLCDKSGAAKPALSTLLELK
                     RAYVG"
BASE COUNT         1191 a         2447 c         2443 g         1195 t
ORIGIN      
        1 gaacgaggag cacctcgttg tcgagcccga gctcgccggg atcctgaatt ccgttgccgt
       61 tggtatcgtc atacatttgc ccgacgatcg agccaaggtg ccacgccgcc aggtcgatgc
      121 cccccagctg ctcatccaca ccaagcgtaa cggccacggg ctcggcgccc ggccgcggac
      181 ccacttcgat ttcgatcgtg tagtcccccg tgtcttccac gatatcgccg ctcccttcga
      241 caaatgggtc gtaaaggcca ttggcgaaac tggcaaggcc ggcgtagtac aggccgtcgg
      301 ccttggcgac gaattcgagg aatggaccgg ccggcccgcc gccgtcgtgc cccagcagtt
      361 gcgcgccgct ggcgtcgaac agccgcagca tggaagccag ctccgagccg aattcccagg
      421 tatcgacgtc gatccgcagc cggtcgccgg cgttgagctg gacctgaaac atgtccacgt
      481 ccagcagcgg atcaattctc ttgttgtcgc cgatggtgcc gcgggcgata taggtgcccg
      541 gtgtggcgga cgacaggccg gtggccgtcg ccatggaaat cgtatcgttg cgttcagcca
      601 ccagcggcag cgatgcatca ggcgggatga cctcgatctc gtgcggctcc acctcttccg
      661 cttggatcca cgccagcggc agctgctgct gaacgaagta cttgccggcc ggcaggccac
      721 cgaaggcata accgccgccc ccgtcggtcg tttgctcggc taccagcggg tcggcggcat
      781 cgaatacgcc atccccgtcg tctgcgtaaa gctggacacc ccagtcggcc agcggcagat
      841 catccggggt gatgccatcg ccggggatgt cttcgaaaac agcgccgccg atcatgccgg
      901 cggtcagcag acagcgacct tccagcggct ccagaaaaag ttgccgggcg ctgtagcgct
      961 ggctgctggc ggaatgacga cgtggcgcag aatcacgaac aaaggcagcg ctcatgtttt
     1021 cctcgtgcaa agaatggagt ttgctgaacg tgtcgctgcg gcgacagccg ccgccacggc
     1081 gtcgatcatt cctcgccatt gcggacttcg atcgccgggc tgacgcgggc gatatcgagt
     1141 ccgtccccca tgtcggcgag ggcgaagaac acagggaact tccccagcag caacagcgaa
     1201 gcctccaaga ggtcgtcgcc gccgagcagg ttgatgtgga ccgttatcga accggtgcca
     1261 aagaactgga aatcggcgct cacctggtcc gcccgctcgc tgccgagctg acggatttcg
     1321 aggcttccgc tgaccgaact cgcgcacgcc atgtccattc gctcggtccc tgcgctgccg
     1381 tcctggagca ggttgaccga tccgccggcc agcacgaccc aaatcgccga tttgctccat
     1441 cgtgcagagg taagctctcg gctccaggcc ttcgagacgg ggacggaaca aggggggccg
     1501 ccggcggccg cgggacgagc gctgcatggc aagttccttc ggggaggcag gtaatgtgat
     1561 tgacccgaat tgcatgccgg gcccgaattc aagtgccgcc agacggtgtc aacgaaacac
     1621 gcatcccgag atttcctgac ggcccaggta ctgtcgcctg gacaatggca cgccgcggtc
     1681 cgctcaacta cagtggacgg ccgctcctgt gccgcacagc cttggtagcc gtggggatga
     1741 ccgctccttt tgccgacctg ctcgaacgtg ctcgccgggg cgattccaac gcgctggctg
     1801 cgctggccag ccagtacgag ccggaggtgc gagtgatggc ccgcgtcctg ctcggaccgg
     1861 cgctgcggcc gtatctcgat tcgctggacc tcgtgcaatc ggtccatcgc agcctgctgg
     1921 ccggcctccg ccacgggaaa atcgatctca cgaacccgca gcggctggtg gccctggccc
     1981 tgacgatggt ccgccgcaag atcgcccgtc agtggcggcg gcaccagcgg caggagcggc
     2041 cggtcggcgg cgatgccgag acactggccg atcggctggc caccctatgc agcgccgaag
     2101 acgatcccgc tcgccgtgcc gagcgaaacg actcgctccg gctggtgtgc cggcagctca
     2161 ccgagaccga gcggcggcta atcgagctgc gattgaacgg gtgcagcacg atcgaggccg
     2221 caggtcagct gggccttgac gccgatgtgc tccgcgtccg tctcagccgc ttgcggcagc
     2281 gccttcagca agctggaata cttgaggagt tcgtctagct cgccgactgg cggccggctt
     2341 cggccaggcc gcggaggatg gcgtcggcga cggcggcggg gtcgatctcc agggcgactt
     2401 cgatatcggc ccggtggggg caattcgggc ggcggtcgaa gatcgtcgcg cccatggtga
     2461 tttcgccgtt ggtttcgaca tcgccggcca gctcctccat gcgaaacagc tccgggtgca
     2521 ggacggcggc catcgccacc gcatcgggca ggcggatgct ctccaggccc aggtgctgcc
     2581 ggtgggtgcg aaacaggtag ggcagcgcta gccggagcag cttgccggcg cgggtcgttt
     2641 cgtcgggcag cttgtcgagg aaatcgaacg accagacgac ctgatcggtc acatcgaggg
     2701 gaatcagcgt tttggtcagc ggcgacttga agaccgcccg ggcgctgatc gggtcggcgt
     2761 ggatgttgaa ttcggcgcaa ggcgtcgcgt tgccgacgca gcggacgctg ccgcccgcga
     2821 tcacaagccg gccggcctgg tttacgaaat ccgggtcgcg ggtcagcgcg cgggccacgt
     2881 tcgtcagcgg gccgaggcag acgatcgtca cgtcgccggg ggcgatgcgg cattcgtcgg
     2941 ccatcagttt ttcggagggg tgctggcggg ccagctgcga gacggccagc cccgcgccgc
     3001 ccaggccgtc ggggccgtgc aagtgccggc cgtcggcgta gggtgcgtcg tcgggcgggg
     3061 cggcagcgcc cagccgcgga tagcgcggcg gatcgagccg ctcgacgatc gcctgcacgt
     3121 tttgcgtcgc ccggtcggcc ggaatgttgc cggcgacggc cgtgaccgca acgacctcca
     3181 gccgcggatc gaagagcgcc agggccaggg ccacggcgtc gtcgattccc gggtcgcagt
     3241 cgatgatgac tttccgcggc atgcaactcg ttcgccggat aggaagaatc cttagcgcga
     3301 aaacgatttt agtccctggt cgcggcgcgt cacaaggcgt gcgcgattac aatcgcgcct
     3361 atgatcactt cccagctcgg ccgcatcgcc gccggcgtcg acttgacgca ggacgagatg
     3421 acggaagtca tcgatcagct gctgaccggc gcggtgccgc agaacgagat cgccgtcttg
     3481 ctgacggcct tgcgggccaa gggcgaaacg gccgacgaga tcgccggggc ggcccgggcg
     3541 atgcggcggc acatgacgcc gatccgcacg tcgcggaccg gcctcgtcga tacctgcggc
     3601 accggcggca tcggcagcga cctgtttaac atcagcacgg cggcggccat cgtcgccgcg
     3661 gcggccggcg tgccgatcgc caagcacggc aaccgcagcg tcaccagcaa gagcggctcg
     3721 gccgacgtgc tccgcgagtt gggcgtcaat gtcgatgctc cagtgccggt cgtggagcgc
     3781 tgcctggacg agctgggcat ctgcttttgc ttcgcgccgc agctgcaccc ggccatgaag
     3841 cacgtcgcgc ccgtccgcaa gcagctgggc ttcgcgacga tcttcaacct gctcggtccg
     3901 ctctgcaatc cggccggggc gacgcggcag cttttgggcg ttggccggcg cgagctgcat
     3961 cggaccatgg ccgatgtcac ggcccggctg ggcacggacc acacgctcgt cgtccacggc
     4021 accgacggga tcggcgaagt ctcgctcagc gccccgaccg aagtgatcgc cgtcagccgc
     4081 gagggtcagc agtcgattgc ctggcggccg gaggatttcg ggctgaccac cgccggccgc
     4141 gagtcattgc gcgtcagcag ccctcaggag agcgccgccc tgattcgccg catcttcagc
     4201 ggtgagccgg gcccgcccca cgatgtggtc gtgatcaacg ccgccgcggc gctcctggcc
     4261 gccgaagccc agcgcgaagc tcggagagcc gccgagcaag ccgccgcggc gatcgacagt
     4321 ggagcggcag cgcggctgct tagcgcatgg gcggagatga gttttgatta gcgggcctgg
     4381 agccatcgaa gccgcacccg ctgcgcgcga gtgtagcccg ggccggcctg ggcgcccgtg
     4441 agctgctggt ccccttcgcc cggcacgatc cagatgttgc ggaaggccac gggattgccg
     4501 tggtcctgca ggttgatcgg aaacggctcg gggccttcga cttttccgcc gccggttttg
     4561 gcagtgatcg cccggtgcca atgaatcggt acgccgttat gcagcgcggt gaggcgggca
     4621 tcggcgagct tcgtctggcc gccagccgcg aataccggcg gcgtgaacca gatgtcgtag
     4681 gtttgccagg ccagcggcgg caggcacatg ttcacgtcgg gccgcgtctg ccgatacagc
     4741 ccgccgcatt cgttctcgac cccttccagg ccgaacgaat cgagaatctg caattcgtac
     4801 cgctgctgga tgtaaacgcc gctgttgccg cgggcctggc cgcgggccgc gggcatgaac
     4861 ggcgtgcgga attccaggtg caggtggaag gcacttaccg gcatcttggt tagcacgccc
     4921 gccagcaaga ggccatccgg cgtgaccttg gccccttgca actgctcggt cgagccgccg
     4981 ccaaacagca cgatcgcacc gggcggcggc gccagcccca tggtcgggct gctccgctcg
     5041 actttaccca gccggccgag cagccggcct gcggcatcga agctcagggc ccgcgtgccg
     5101 tccacgacaa actcgccggc cggtccgctc agcgtgagcc tgccatccac cgcctcgccc
     5161 gagcagccgg ctttcgtcgc ccgatcccag cccacacccg gcaaaccgcc gcgataacaa
     5221 accgcgtcaa acttaccgcc gccgagcgcg acgacctgca ggcccgccgg ttcaaacccg
     5281 tagccgggaa tgaagagcga cccggcgtat tcgccctgca agacgtagtc gatgcccgcc
     5341 tgggccgcct ccgtgaaggt ccccggacgg gcgggctgcg catgcccggc gtcagccgtg
     5401 cataacagca atgcgattgt caggagagcg gcagtcagga cgcgcatgga tcactccggc
     5461 ggaaaccact ctagttaaac gtcagagaaa atgccgtgga aaaacatacg accggccccg
     5521 tgcagggcga gccggctcat tcggcgggcc gtggttcgag cgagcgaacc acggccagtc
     5581 ttgccgatta ctccgcgcaa gactaccgct tgccggccgg gtattgatgc ccggtcgccg
     5641 tcatcggcac cgcgtacagc gacgtacggg cggtcacata cagcgtcttg ttgtccctcc
     5701 cgccgaacgt gcaattcgcc ggctgctcgg gcagctcgat gattcccagc gctttgccgt
     5761 cgggactgaa gacctgcagg cctagagccg tcgcgatgta gaggttgccc ttggagtcga
     5821 tggtcattcc gtcgccgccg ccagccgtct tgcccggggc ctgcttgagc gtgcagaacg
     5881 tccgctgggc ccccagcttg ccgaggcctt cgaccggata cgccagcatc tcggcctgca
     5941 gcgagggaat gacgtagagc gtcttctcat ccggcgagag cttcacgccg ttgggggcat
     6001 tctcgatgac gcccagccgc gtgaccttgc cgtcggccgc gcggtagtag aacgcccgca
     6061 cgccctgcgg cagcggcatc ggggcgcgga agtgcggatc ggtgaaatag acgccgccct
     6121 ccttgtcgat caccaggtcg tttggcgcgt tgaaccgctt gccttcatag ccctcggcga
     6181 gcgctttgac ctccttggtc ttcgggttga cctcgatcag ccgcccgtcc atttcgcagg
     6241 cgacgatgtt gccggcggcg ttgaccatca ggccgttggt gtgcttcgac ggatcgagga
     6301 ccgtcgtgag ctcgccggtc gcatcgacct tgtggagctt gttgccggcc acgtcgctga
     6361 aatagagatt gccctggccg tccgaggccg ggccctccgt gaattgcagg ccggtgtgca
     6421 gcttcttcac ttcgccggcc gggccgatgc cgggaatcgg atcagcggcc gaagcggcgg
     6481 cgaccgccag cagccagccg gctgcggcaa ctgcgaatga gcgatgaatc attggctgtt
     6541 ctcctgcgag ttgctgggat ggaatcttcc gccggccatg ggcgggcggc ggcgtgcctc
     6601 attctatccc acgtacgcgc gcttgagttc cagcagcgtc gagagcgccg gcttggccgc
     6661 accggacttg tcgcagaggc cgctatgggc catttcgtgc ggctgattgt cctgccaggc
     6721 gtcccagatg atcgcctgca ccgactgctt ggcgagcaac agcggcagca cccattgcaa
     6781 gagcacgtgc tgccaggcgg tggtcgggcc gccgggccgc aaatcgatga gcggccggcc
     6841 cggatgccgg gcctgcggat cggccaccgc gctgctcggc gcggacagaa acaccatcag
     6901 cggaatgccg atctgcgacc agcggtcgat ctggcgggcg atctccagcg gatcgcgggg
     6961 cagcgtgccg ccgggccaat agccgtagtg aatctccagc ccgacgccag ccaggcccag
     7021 ctcgccgcgg accagcgtgt cggcgaagtg cagcggcgtc agctcctggt ccttgcggac
     7081 gatgtactcg ccccagggct gcgcgaagct catcaccagc ggcgtccgcg gatcgacggc
     7141 ccgcacgcga tcgaccgcct ccacgaccag ccgcagccgc tgctcttccg agaattcgag
     7201 cgccccatcg aggttcattc cggccgcgac gtgccacagg ttgaccttgc cgcggtagcg
     7261 tttcacgacc tcctca
//
LOCUS       DASRJB010000122         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2292850, whole genome shotgun sequence.
ACCESSION   DASRJB010000122 DASRJB010000000
VERSION     DASRJB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292850"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VFB80_03560"
     CDS             complement(<1..1564)
                     /locus_tag="VFB80_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN32863.1"
                     /translation="MIRPAIVIGLAAAAWFAGVHRAAAYPVGPSLSLEELIAQADFVG
                     KVTVLESKPLSDAWFDKVAAFEPTETRLKVLATYKGKAGGETIAFRHYGPANESQPYM
                     YMPQFYRFERGRTYILFAAKTEQEGVFRQLWKQHKAQEDQGVFLAADTEPRVDLSIKE
                     AIFAELTGLLKSAAAADVKYGLSHLDLLSGGTYHGQKDFDREEVLDHAKRLLNHKDNE
                     VVVAAVRVMGSNNPYMSSEYIPGWLATIGRGDIPGFGAWDRSQENLGGRLYWKQLAAI
                     ADSPAPAATRGLAIRALGRAEEPAILPLAQRWTADADPQVRQAATVLLGDFKGDFDPA
                     VFAKLAADAQPAVRVGAAQAIGLGQFREQIPTLGKLLADGDPDVQAAAALSLLSFSTA
                     DSGDVLRANLKHPQFHPLFVNALARADAGRYLEELAEIVKKKKEPESFWGGRIPWGVS
                     WELLFFYVQRQPPAQVRGGQLDKVLDALEYPASGDPKGPSYYSSSEPRDLYALYLQRG
                     MTERAAKFRALAK"
     gene            1752..2165
                     /locus_tag="VFB80_03565"
     CDS             1752..2165
                     /locus_tag="VFB80_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32864.1"
                     /translation="MTRLQDFVAKKLSDRKICDAEVPAIRERLYADGQLNLDDVKLLI
                     QLYCDADHRAPAFEKLFFSVLEEVLLEDGEIQPAEQFYLLKMLYSDRKICEPEREFLR
                     NVRRKQKVRVPEFEALCKTALAASDKNWSVGGRSR"
     gene            2339..3481
                     /gene="aroC"
                     /locus_tag="VFB80_03570"
     CDS             2339..3481
                     /gene="aroC"
                     /locus_tag="VFB80_03570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZN32865.1"
                     /translation="MTLRYWTAGESHGKTLLAVVDGFPAGVQIDTGPIDVELRRRQGG
                     YGRGGRQRIETDTVELLSGVWRNVTIGSPIGLQVVNKDYKLERLSDLARPRPGHGDLT
                     GAIKYLGPIRGILERASARETTVRVAAGALCKQLLAVFGITAIGYVVELGGVKLDVPE
                     LPLAERVKLRDASDIYALDAAREQQVKDLIDETGKAGDTLGGVVEVRVEGLPFGLGTH
                     AQWDLKLDGRIAQAVMAVQAIKGVEIGLGFEAARRRGSQVHDPIHFDAAKRDTKTLGY
                     ERPTNNAGGLEAGMTNGQPLVVRAAKKPISTLRKPLDSINLQTKQPEPAEYERSDICA
                     VPAASVIVENVVAFEIARALVEKFGNDSLAEMQARYELFLKMARER"
     gene            3478..>4012
                     /locus_tag="VFB80_03575"
     CDS             3478..>4012
                     /locus_tag="VFB80_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32866.1"
                     /translation="MTASTRILLCRIGCVALCLVPTALVAGWTCWRASASFAVAQRAD
                     WERELTSRFGLVAEIGGVSYPSPGLARLDNFRLLDPESRQLVLSAAIVEVAAHEGGWQ
                     VELWQSQVQASGLGSLARAIEDRILRSPESLARCEISARELSIVRGSAAQSLVQIAAQ
                     LSPRPEGSLLTCEFHLPA"
BASE COUNT          674 a         1347 c         1323 g          668 t
ORIGIN      
        1 tcttggccag cgcccgaaat ttggcggccc gctcggtcat cccgcgctgc aggtagaggg
       61 catacaggtc gcgcggctcc gacgagctgt agtagctcgg acccttgggg tcgccggagg
      121 cggggtactc gagcgcgtcc aagaccttgt cgagctgccc gccgcgaacc tgcgcgggcg
      181 gctgccgctg cacgtaaaag aacagcagct cccagcttac gccccaggga atccggccgc
      241 cccagaagga ttcgggctct ttcttcttct tgacgatctc ggccagctcc tcgagatacc
      301 gaccggcgtc ggcgcgggcc agcgcgttga cgaagagcgg atggaactgc ggatgcttga
      361 ggttggcccg cagcacgtcg ccgctgtccg cagtcgagaa cgagagcagg ctcagcgccg
      421 cggcggcctg tacgtccgga tcgccgtcgg ccagcagctt gccgagagtc ggaatctgct
      481 cccggaattg acccaggccg atcgcctgcg ccgcgcccac ccgcaccgcc ggctgcgcgt
      541 cggcggcgag cttcgcgaac accgcggggt cgaaatcacc tttgaaatcc cccagcagca
      601 cggtcgctgc ctgccgcacc tgcggatcgg cgtcggccgt ccagcgctgc gccagcggca
      661 aaatcgccgg ctcctcggcc cgacccagcg cgcgaatggc aagcccgcgg gttgccgcgg
      721 gggccgggct gtcggcgatg gccgccagct gcttccagta cagcctgccg cccaggtttt
      781 cctgcgagcg atcccacgcg ccgaagcccg gaatgtcgcc gcggccgatc gtcgccagcc
      841 agccgggtat gtattcgctc gacatgtacg ggttgttcga tcccatcacg cgcactgccg
      901 ccaccacgac ttcattgtct ttgtgattca gcaggcgctt ggcgtggtcc agcacctctt
      961 cgcggtcaaa atccttctgg ccgtgatagg tgccgccgct gagcagatcg aggtgcgaca
     1021 ggccgtactt cacgtccgcc gccgccgcgc tcttcagcag gccggtcagc tcggcgaaga
     1081 tcgcctcctt gatcgacagg tccacgcgcg gctccgtatc ggccgcgagg aacaccccct
     1141 ggtcctcctg cgccttgtgc tgcttccaga gctggcgaaa gacgccctcc tgttccgtct
     1201 tcgcggcgaa gaggatgtag gtccgcccgc gctcgaaacg gtagaactgc ggcatgtaca
     1261 tatacggctg cgactcgttc gcggggccgt aatgccgaaa ggcgatcgtc tcaccgcccg
     1321 ctttgccctt gtaggtcgcc agcaccttga gccgcgtctc ggtcggctcg aacgctgcga
     1381 ccttgtcgaa ccaggcgtcc gagagcggct tcgattccag caccgtgact ttgcccacga
     1441 aatccgcctg ggcgatgagt tcctcgagcg acagcgacgg cccgaccgga taggctgcgg
     1501 cgcggtggac cccggcgaac caggccgcgg ctgccagacc gatgacgatt gccggtcgga
     1561 tcatgggaag gctccgtgtc agcgcgagag gagtttcaaa attagacgat tttgctctgc
     1621 cgacggatct gccggagcgg ttgtagcgat cgtatcagcc ttcccacgga cagcccacct
     1681 ttgggcatgg cgtaacctaa ggtttcccgt tgccggatcg ttccaaccca ttcgacgcgg
     1741 gagccccgac gatgacccga ttgcaagact ttgtggccaa gaagctgtcc gatcgcaaaa
     1801 tctgcgatgc tgaagtgccc gcgatccgcg agcggctcta tgctgacggc cagctcaacc
     1861 tcgacgacgt caagctgctc atccaactct attgcgacgc cgaccaccgc gccccggcct
     1921 tcgagaagct gttcttctcg gtgctggagg aggtgctgct cgaggacggc gagatccagc
     1981 ccgccgagca gttctatctc ctgaagatgc tctactcgga tcggaagatc tgtgagccgg
     2041 agcgggaatt cctgcggaac gtccgccgca agcagaaggt gcgggtgccg gagttcgaag
     2101 ccctctgcaa gaccgccctg gccgctagcg acaagaactg gagcgtcggc ggccgcagcc
     2161 gctaaacggc acatcgatcc gctcgtctcg gcccatatca acccgctccc aacgtctcgt
     2221 gggagcgggc gtctgccggc tgctctatgg ccgaaggccg ctgagaatta gaattcgagt
     2281 tggctgacca ggccggtcgc ggctcgggag tgctaatctg cgcaagtatt gaattgcaat
     2341 gaccttacgt tattggacgg ccggtgaatc ccacggcaag acgctgctag cggtcgtcga
     2401 tggttttccg gctggcgtcc agatcgatac gggaccgatc gacgtcgagc tgcggcggcg
     2461 gcaaggaggc tatggccgcg ggggccggca gaggatcgaa accgacacgg tcgaattgct
     2521 gagcggtgtc tggcgcaacg tgacgatcgg cagccccatc ggcctgcaag tcgtcaacaa
     2581 agactacaag ctcgagcggc tgagcgacct ggcgcgtccg cggcccggcc acggcgacct
     2641 caccggcgcg atcaagtacc tggggccgat ccgcggcatc ctggagcggg ccagcgcccg
     2701 cgagaccacc gtccgcgtcg ccgccggtgc gctctgcaag caactgctcg cggtgtttgg
     2761 catcacggcg atcggctacg tcgtcgagct gggcggtgtc aagctggacg tgccggagct
     2821 gccgctggcc gagcgcgtca agctgcgcga cgccagcgac atctacgcgc tcgacgcggc
     2881 ccgcgagcag caggtgaagg acctgatcga cgaaaccggc aaggcgggcg acacgctcgg
     2941 cggcgtcgtc gaggtccgcg tcgaagggct gccgttcggc ctgggcacac acgcccagtg
     3001 ggacctcaag ctcgatggcc gcatcgcgca ggccgtcatg gccgtacagg cgatcaaggg
     3061 cgtcgagatc ggcctgggct tcgaggccgc ccgccgccgc ggctcacagg tccacgatcc
     3121 gatccatttc gacgccgcga agcgcgacac caagacgctg ggctacgagc ggccgacgaa
     3181 caacgccgga ggactcgaag ccggcatgac caacggtcag ccgctcgtcg tccgggccgc
     3241 gaagaagccg atcagcacgc tccgcaagcc gctggattcg atcaacctcc agacgaagca
     3301 gcccgagccg gccgagtacg agcggagcga catctgcgcc gtgccggcgg ccagcgtgat
     3361 cgtcgagaac gtcgtggcct tcgagatcgc ccgcgctctg gtcgaaaaat tcggcaacga
     3421 cagcctcgcc gagatgcagg cccgctacga gctgtttctg aaaatggcga gggagcgatg
     3481 acggcctcca ctcgcatcct gctctgccgc atcggctgcg tcgcgctctg cctcgtcccg
     3541 acggcgctgg tcgccggctg gacctgctgg cgggcttccg caagtttcgc cgtggcccag
     3601 cgagcggatt gggagcgcga gctgaccagc cgcttcgggc tcgtcgccga aatcggcggc
     3661 gtcagctatc ccagtccagg cctggcgcgg ctcgacaact tcaggctgct cgatccggaa
     3721 tcgcggcagc tcgtgctatc ggcggcgatc gtggaagtcg cggcgcatga gggaggctgg
     3781 caagtcgaat tgtggcagtc gcaggtgcag gcgtccgggc ttggctcgct ggctcgcgcg
     3841 atcgaggacc gcatcctgcg ctcgcccgag tcgctggccc gctgcgaaat ctccgcccgc
     3901 gagctgtcga tcgtccgcgg ttcggcggct cagtcgctgg tgcaaattgc ggcgcagctt
     3961 tcgccgcggc cggagggctc gctgctgacg tgcgaatttc acctgccggc cg
//
LOCUS       DASRJB010000123         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_36712, whole genome shotgun sequence.
ACCESSION   DASRJB010000123 DASRJB010000000
VERSION     DASRJB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36712"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1217)
                     /locus_tag="VFB80_03580"
     CDS             complement(201..1217)
                     /locus_tag="VFB80_03580"
                     /inference="COORDINATES: protein motif:HMM:NF042558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6666 family protein"
                     /protein_id="HZN32867.1"
                     /translation="MTGRAIAAAIAILAAAGSAAAAGWMPSIDTDQSFTDLTAPADVS
                     PWGECCNDCIGNWWDNTALFFASDAWRTRADDDRPGNFGFRTGFNTGFGGWWDRPIRM
                     QLGASFAGYDLTGRDGDFGADPDTNASFEQQIFATFGFYKRSDVCNGERFAWAVAVDL
                     LYDNHLGEEAEEVFNRQARGYLGYAWDEANEFGTWFAFQLNWARYVSNTRGRTRVRGL
                     DQVNFFWHHNWDFGGDTWFYLGGAEEPGEWTLGLLGQAPLNPSVALFGGFTYVIPSAP
                     SGDPFPGLNQHYAEEYWNVSFGIVWYPGCKAANPSVSGQRGLPLLPLADNGSFLLKAG
                     TGDL"
     gene            complement(1385..1852)
                     /locus_tag="VFB80_03585"
     CDS             complement(1385..1852)
                     /locus_tag="VFB80_03585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZN32868.1"
                     /translation="MKSIAGQEVDLAQYKGKVVMIVNVASKCGLTPQYNELEALYEKY
                     KDKGVVVLGFPCNQFGKQEPGTDAEIAEFCSETYKVKFPMFSKIDVNGKDAAPLYKLL
                     TSSETQPKGKGDISWNFEKFLIGKDGQIAARFAPRTEPDAKEVVAKIEAELAK"
     gene            complement(2071..2955)
                     /locus_tag="VFB80_03590"
     CDS             complement(2071..2955)
                     /locus_tag="VFB80_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32869.1"
                     /translation="MTTQLAWKALSLVSHLHAAEAVARQRQLADERLAAALAEPAALL
                     AGEIRIAHLPAGRFWRHLIPLAATLDSRRQLVETAVVKVAGRDSRTEPAVVPITAALA
                     AVETAAGAALPKFAEEIALRERPLREQWEARGPGLLTQIGWLTDPALLPESCPVLLVH
                     PTLGGGGAAHLSYNSVRIEAVLANPIPELPEVVRLAWLIAQLQLDLPMWSEALHAERL
                     PHVAQYAMLPATLAAAESVELARLTPETLRLAVDSWNLSVPKGVDASTLIFDWWQTYI
                     ADRPPFRVALAALDQMFG"
     gene            3053..4156
                     /locus_tag="VFB80_03595"
     CDS             3053..4156
                     /locus_tag="VFB80_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HZN32870.1"
                     /translation="MTSFPPLGTPKDELDTPALCIDLDALEANIADAAALCRNCGVAW
                     RPHAKCHKSPDIARRLLEAGAIGLTCAKLGEAEVFAAAGIADLLIANFIAGPRKIERL
                     VELRRRADPLICLDHVDQAGPISRAFAAANMKVRALLEIDIGMQRAGVQPGKPALELA
                     KRVAELPGIELAGVMGYEGHLLLIEDQGEKAARICAALGQLVETKERLEQAGIACPIV
                     SCGGTGSLTFAARQPGITEVQAGGLIFMDAFYRHKCRVEGYRYALTLLTTVVSRPAAD
                     RAIIDAGRKTMNIELHLPIVMDREGIEVRALSAEHGILQLAPAARDLKIGDRLELIPG
                     YGDFTTVLHNQFYVLHHNRLIDIWRLAARGRLQ"
     gene            complement(4169..4756)
                     /locus_tag="VFB80_03600"
     CDS             complement(4169..4756)
                     /locus_tag="VFB80_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN32871.1"
                     /translation="MSVDIALRIDDAEGMHRLAAYVSWVLIAAGLAGCSGGASPPKKV
                     IATSPDGPAKVLPLTAAAKPMTWPTSTVAPAAYEEPLAVRPAAIAVPVVKPLAQWTEQ
                     DVAADALGRIGAAAVPALIEGLRSPDPAVRLKAVEVLGRMGPDAQDAVPDLVRLLDDP
                     DEAIRKAATRTLGRIGPPAQEAVPALVRTLLQPTP"
     gene            4863..5489
                     /locus_tag="VFB80_03605"
     CDS             4863..5489
                     /locus_tag="VFB80_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32872.1"
                     /translation="MFGFTGFHRSSSPFEQTGSVELNTNYCTRRKPLWLIALKFLGTA
                     AMIAVGAAIAVPTLLEFQVPLWEAVGYTAAGMLVYTLIAFFFRPEPNTDNMGWGGGLG
                     NDPFQSSDNVNRFLWRLHCVLGPGRFTAETFLDALVFIGLLRGDAEEECAPPGQSFAA
                     SGQADGFDATRPIAPLDPDRFAHTSANSVTERIQHARLSSSNPPAATR"
     gene            5599..>6350
                     /locus_tag="VFB80_03610"
     CDS             5599..>6350
                     /locus_tag="VFB80_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32873.1"
                     /translation="MGSAFALCRRPVRAAFTLVELLVVIAIIGVLVALLLPAVQAARA
                     AARRMSCQNNVKQMGLALHNHHDVYGTFPAGGVTNGPCCGTESGGTWTIFILPYIEQM
                     NLYERYNFTQTNENAANAFVRTQHVKPYSCPDDPLIRQLMIPASGPGANANLNYATGS
                     YRAMAGVTDGLGWFDAECGRGYPLGQRGILHSTSDPKSPPAYQAGYVAPPYDKERMAN
                     ITDGTSNTLMVGEYYTGPKSTPRRSTFWAYTYT"
BASE COUNT         1063 a         2157 c         2074 g         1056 t
ORIGIN      
        1 tgctccaccc gcgtgctgta gacgcggagg gtcggacccg ccggacccac cgtgccgacg
       61 ctgccacaca gcacgtacgc ggcggagtaa atcgcaagca ggaacaaaaa ggcaactacc
      121 gcgacgagcg ggcctgaaac ggagctgcgc gagtcttcca tcgctggatt gtaagcggcc
      181 ggcagccgcg gacgccaatt ttacaggtcg cccgtgccgg ctttcagcag gaagctgccg
      241 ttgtcggcca gcggcaagag cggcaggccg cgctggccgg aaacgctggg attggcggcc
      301 ttgcagccgg gataccagac gatgccgaag ctgacgttcc agtattcctc ggcgtagtgc
      361 tggttcagtc cggggaaagg atcgcccgag ggggcgctgg ggatgacgta ggtgaagccg
      421 ccgaacaggg ccaccgacgg gttcagcggg gcctggccca gcaggccgag cgtccactcg
      481 ccgggctctt ctgccccgcc gagatagaac caggtgtcgc cgccgaaatc ccaattgtgg
      541 tgccagaaga aattgacctg gtcgaggccg cgaacgcgag tgcggccgcg cgtgttgctg
      601 acgtagcggg cccagttgag ctgaaacgcg aaccaggtcc caaattcgtt ggcctcgtcc
      661 caggcgtagc ccaggtagcc gcgggcctgg cggttgaaga cctcttcggc ctcctcgccg
      721 aggtgattgt cgtagagcag atcgaccgcg accgcccagg caaaccgctc gccattgcac
      781 acgtcgctgc gtttgtagaa gccgaaggtc gcgaagatct gctgctcgaa cgaggcgttc
      841 gtgtcgggat ccgcgccgaa gtcgccgtcg cggcccgtga gatcgtagcc ggcgaagctc
      901 gcgcccagct gcatgcggat gggtcggtcc caccagcccc caaagcccgt gttgaagccc
      961 gtgcggaagc caaagttgcc gggacggtcg tcgtcggccc gcgtccgcca ggcatcggag
     1021 gcaaagaaga gcgccgtgtt gtcccaccag ttgccgatgc agtcgttgca gcattcgccc
     1081 caaggcgaga cgtcggccgg cgcggtgaga tcggtgaagc tctggtcggt gtcgatcgac
     1141 ggcatccagc ccgctgccgc agcgctgccg gctgccgcca ggatcgcaat cgccgctgct
     1201 atcgctcgcc cggtcatcga cgccctccct tggcttccgc tgggatcgac ccgtcagaac
     1261 cggcaaatcc atttcacgcg gcacgctcgg cagggatgcc gcaggcggca aggtttgcca
     1321 gcgcatgaaa agccccgcgc caattgggcg gggcaggttc gctagcgagg gtgagcaagc
     1381 gtggttactt ggccagctcg gcttcgatct tggcgacgac ttccttcgcg tcgggctcag
     1441 tccgcggcgc gaaacgggcg gcgatctgac cgtccttgcc gatcaggaat ttttcaaagt
     1501 tccaagagat gtcgcccttg cccttgggct gtgtctcgct cgaggtgagc agcttgtaga
     1561 gcggcgcggc gtccttgccg ttcacgtcga tcttcgagaa catcgggaac ttcaccttgt
     1621 atgtctccga gcagaactct gcaatctcgg catcggtccc cggctcctgc ttgccaaact
     1681 ggttgcaggg gaagccgagc accacgacgc ccttgtcctt gtacttttcg tagagggcct
     1741 ccagctcgtt gtactgcggt gtgaggccgc acttgctcgc cacattgacg atcatcacca
     1801 ccttgccctt gtactgggcc agatcaactt cctgcccggc aatcgacttc atcttgaagg
     1861 cgagcggacc ggctttctcg tccgccttcg ccgctgtcac gcaggcggcg acgatgccgg
     1921 ccattaagag ggtgctgccc catttcataa cgctgactcc tgttccgatt gactggtggg
     1981 tgggagaggc cgcggcgctc gcctgcgggt gaccttaaga tagccgaata aacgccgatt
     2041 tccaggggcg ctgcgggctc cggcatccgc ctagccgaac atctgatcga gcgccgccaa
     2101 cgccacgcgg aacggcgggc ggtcggcgat atacgtctgc caccagtcga agatcagcgt
     2161 ggaggcatcg acacccttcg gtacggacag attccacgaa tcaaccgcca gccgcagggt
     2221 ctctggcgtc agccgggcca actcgacgct ttccgcggcg gccagcgtcg ccggcagcat
     2281 cgcatactgg gcgacgtgcg gcagccgctc ggcgtggagg gcctcgctcc acatgggcag
     2341 gtcgagctgc agctgggcga ttagccaggc caggcgcacc acctcgggca attcggggat
     2401 cggattggcc agcaccgctt cgatgcggac cgagttgtac gacaggtgcg ccgcgccgcc
     2461 gccgccgagc gtcggatgaa ccaggagcac cggacagctc tcgggcaaca gcgccggatc
     2521 ggtcagccag ccgatctgcg tcagtagtcc ggggccgcgg gcttcccact gctcacgaag
     2581 cggccgctcg cgcagcgcga tctcttcggc gaacttcggc agcgccgcgc cggcggccgt
     2641 ttccactgcg gcgagcgccg ccgtgatcgg cacgaccgcc ggctcggtgc gcgagtcgcg
     2701 gccggcgacc ttgaccacgg ccgtctcgac cagctgccgc cggctgtcga gcgtggcggc
     2761 cagcggaatc aggtgccgcc agaaacgccc cgcgggcaag tgggcgatcc ggatttcgcc
     2821 cgccaacagc gcggcgggct cggccagcgc cgcggccagc cgctcgtcgg ctagctgccg
     2881 ctggcgagcg accgcctcgg ccgcatgcaa gtggctgacg agcgaaaggg ccttccaggc
     2941 gagttgggtc gtcatggcgg atttccgcga tcggagcgac agaattcacc cgtccggcta
     3001 tcataaaccc gacgaataca cgtcaactct agcttgggct agcacggcct gcatgaccag
     3061 ttttccgccg ctaggcacgc cgaaagacga gctggacacg ccggccctgt gcatcgacct
     3121 ggacgcgctg gaagccaaca tcgccgatgc ggccgctctc tgccgtaact gcggtgtcgc
     3181 ctggcggccg cacgccaagt gccacaagtc gcccgatatc gcccgccggc tgctcgaagc
     3241 cggagcgatc ggcctgacct gcgccaagct aggcgaggcc gaggttttcg ccgccgccgg
     3301 aatcgccgac ctgctcatcg cgaactttat tgccgggccg cggaaaatcg agcggctggt
     3361 cgagctgcgc cgccgggccg atccgctgat ttgcctggac cacgtcgacc aggccgggcc
     3421 aatcagccgg gcattcgccg cggcgaacat gaaggtgcgg gccctgctgg agatcgatat
     3481 cggcatgcag cgggcgggcg tccagcctgg taagccggcg ctggagctcg cgaagcgcgt
     3541 cgccgagttg cccggcatcg agttggctgg cgtcatgggc tacgaggggc atttgctgct
     3601 catcgaggac cagggcgaaa aggcagcccg gatttgcgcc gcgctcggcc aactcgtcga
     3661 gacgaaggag cgcctggagc aggcgggtat cgcctgcccg atcgtctctt gcggcggcac
     3721 tggctcgctg acgttcgccg cgcgccagcc ggggatcacg gaggtccagg ccggcggcct
     3781 gattttcatg gacgcgtttt accggcacaa atgccgcgtc gaagggtacc gctatgccct
     3841 cacgctgctg accacggtgg tcagccggcc cgccgcggac cgggcgatca tcgacgccgg
     3901 ccgcaagacg atgaacatcg aattgcacct gccgatcgtg atggaccgcg agggaatcga
     3961 ggtccgcgcc ctctcggccg aacacggcat cctgcaactc gcccccgccg cacgcgacct
     4021 gaagatcggc gaccgcctgg agctgatccc cggttacggc gatttcacga ccgttctgca
     4081 caaccagttc tacgttctcc accacaaccg ccttatcgac atctggcggc tggcggcccg
     4141 ggggcgactg caatagcggt ggacgaacct aaggcgtcgg ctgtagcagc gtccgcacca
     4201 gggcgggcac cgcttcctgg gccggcgggc cgatgcggcc gagcgtccgc gtcgcggctt
     4261 tgcggatggc ttcatccgga tcgtcgagca gccgcacgag atcgggcacg gcgtcctgcg
     4321 cgtcgggtcc catccgcccg agcacttcga ccgccttcag ccgcacggcg ggatcgggac
     4381 tgcggagccc ttcgatcaaa gccgggacgg cggcggcgcc gatccggccc agggcatccg
     4441 ccgcgacatc ctgctcagtc cactgcgcca gaggcttcac gacgggcacg gcgatcgctg
     4501 cgggcctcac cgcgagcggt tcctcgtagg ccgcgggtgc gaccgtgctc gtcggccagg
     4561 tcatcggctt cgccgccgcg gtcagcggaa gcaccttcgc cgggccgtcc ggcgacgtgg
     4621 cgatgacctt cttcggcgga ctcgcaccgc cgctacagcc cgcgaggccg gccgcaatca
     4681 aaacccagga gacataagct gcgagtcggt gcatcccttc agcatcgtcg atccgcaggg
     4741 ctatgtccac agacacgaac cctgttgcct ttcggcaaca tggcaaaacc cttgcggaat
     4801 tccgcccccg tgcagcgctg cagccgcggc gcgtgctagg cttccgaggg ggagggtaag
     4861 gcatgtttgg attcacggga tttcatcgca gcagcagtcc cttcgagcag acgggctccg
     4921 ttgagctcaa caccaattac tgcacccgca ggaagccgct ctggctgatc gccctcaagt
     4981 ttctggggac ggccgcgatg attgcggtgg gcgccgccat cgccgttccg acgctgctgg
     5041 agttccaggt tccgctgtgg gaggcggtcg gatacaccgc cgccggaatg ctcgtctaca
     5101 cgctcatcgc gttcttcttc cgcccggagc cgaacaccga caacatgggt tggggcggcg
     5161 gcctgggcaa cgacccgttt caatcgtccg acaacgtgaa ccgcttcctg tggcggctgc
     5221 actgcgtcct cggtcccggt cggttcaccg ccgagacctt cctcgacgcc ttggtcttca
     5281 tcggcttgct ccggggcgat gcggaggagg agtgcgctcc gcccggccag tcgttcgcgg
     5341 ccagcggcca ggccgacggc ttcgacgcca cgcggccgat cgcgccgctt gatcccgacc
     5401 gcttcgccca cacgtcggcc aattcggtga ccgagcggat tcagcacgcg cggctctctt
     5461 ccagcaaccc gcccgcggca acgcgctagc tgcgtcgcgc tggttcgcca ggctgatttc
     5521 ttgtatttcg ccggctggtc cgataggatc gcaagcacta gtcttgcgcg cacgttcgtc
     5581 ttgccaggag ccctgctcat gggtagcgcg tttgctttgt gccggcggcc agtccgcgcg
     5641 gcgtttacgc tggtcgagct gctggtcgtg atcgccatca tcggcgtgct ggtcgcactg
     5701 ctgctgccgg cggtgcaggc cgcccgcgcg gccgcgcggc ggatgagctg ccagaacaac
     5761 gtcaagcaga tgggcctggc gctgcacaat caccacgatg tctacggcac attccctgcg
     5821 ggcggtgtca ccaatggccc ttgctgcggc accgagagcg gcggcacctg gacaatcttc
     5881 atcctgccct acatcgagca gatgaacctc tacgagcgat acaacttcac ccagacgaac
     5941 gagaacgccg ccaacgcgtt tgtacggacg cagcacgtca aaccctatag ctgtccggac
     6001 gacccgctga tccggcagct gatgattccg gccagcggtc ccggcgctaa cgcgaacttg
     6061 aactatgcca ctggatccta ccgcgcaatg gcgggtgtca ccgacggcct gggctggttc
     6121 gacgccgaat gcgggcgcgg ctatccgctg ggccagcgcg gcatcctgca cagcacgtcc
     6181 gacccgaagt cgccgccggc ttatcaggcc ggctacgtcg ccccgcccta cgacaaggag
     6241 cggatggcga acatcaccga cggcacctcc aacacgctga tggtcggcga gtactacacc
     6301 ggccccaaat cgacgccgcg ccgctcgaca ttctgggcct acacctacac
//
LOCUS       DASRJB010000124         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_319800, whole genome shotgun sequence.
ACCESSION   DASRJB010000124 DASRJB010000000
VERSION     DASRJB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319800"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFB80_03615"
     CDS             complement(<1..1316)
                     /locus_tag="VFB80_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN32874.1"
                     /translation="MPALALPPLVSRLREAVGVENVLSAQSDLLVYECDGFTIEKNSP
                     DVVVFPRSTADVARVVKIASELGVPFLPRGAGTSLAGGCLPVGGGVMIVLTRMKRILE
                     INLRDRYAVVEPGVVNVWLTNALKGTGYHYAPDPSSQGACTIGGNVATNSGGPHTLKY
                     GVTVNHVLGVEAVLADGRVVRLGGPAGDPLGLDLVGAIVGSEGTLAIVTKVWVRLTRS
                     PEGHRTMLGVFESVDDATAAISEIIGAGIVPAALEMMDQGILVAVEAAFGFGFPLDAQ
                     AILLIEVDGLEAGLDQQRDQIIALCQKCGAREVRLAGDEKERLKLWKSRKQAFGAIGR
                     LSPSYCTQDGVVPRTQLPHILRKIIEIGRRYDIRIVNVFHAGDGNIHPILLFDERNAE
                     QVRRPRSPPGQRPQAAGQALEVPQAGVRGGRPAEPQLLHARRRGAA"
     gene            complement(1395..2600)
                     /gene="lhgO"
                     /locus_tag="VFB80_03620"
     CDS             complement(1395..2600)
                     /gene="lhgO"
                     /locus_tag="VFB80_03620"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HZN32875.1"
                     /translation="MTANRFDVAIVGGGIVGLATAWQLLQRYPRLRPLVLDKEPTVAA
                     HQTGHNSGVLHSGIYYKPGSLRAVNCRSGKRAMEEFCAAHGVPFEICGKVIVATSQSE
                     LPQLEKIYDRGQQNGIRCELVGPERLKELEPHAPGVKAIHVPEAGIVNYAAVSRKLAE
                     LITVGGGEVRCLARVLAVKENGDQIALESTAGEFAADYVVTCAGLHSDRVAKLTGIQP
                     EAQIVPFRGEYFELKHESQHLCRNLIYPVPDPSFPFLGVHFTRMIEGGVECGPNAVLA
                     FAREGYRKSDVNLGDLCETLAFPGFRKLAWKYWRTGAGEMWRSLSKSAFVRALRHLVP
                     EITSEDLVPARSGVRAMAVAPDGAMVDDFVIQPRGRIVNVLNAPSPAATSALNIGKLV
                     VEKLAERLG"
     gene            complement(2597..2911)
                     /locus_tag="VFB80_03625"
     CDS             complement(2597..2911)
                     /locus_tag="VFB80_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HZN32876.1"
                     /translation="MVLLEFSMAPLEKGPSVGNYVARSLEIIERSGLDYRLHAMGTIL
                     EGEIDDVLGVLKQCLEAMAADCDRITCSAKLDFRRGHSGRLSAKVASVEAHLGRELKK
                     VE"
     gene            3133..4113
                     /locus_tag="VFB80_03630"
     CDS             3133..4113
                     /locus_tag="VFB80_03630"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HZN32877.1"
                     /translation="MRVERWLPVVLAALVAWSPPARAQLESPIAPGFEPLPAGETIAP
                     GHIEPTGPPLDYFDLDAWAATQSGPTVPQGDWSWQILPDGIIYRAYLANPKESRIGTT
                     IFWETDEGQWWDSTLGGRAGILRYGTRDTAWPQGWQLDIEGSVQVRLDPEEDRDLRSS
                     DYRFGVPLTYGYGRHRLKIGYYHHCSHLGDEFLEDHPTFLHVNFVRDVFTLGYAYFVT
                     DNLRLYGEIGYGFYVDVSKEWELQFGLEYAPALPTGLRGAPFFAVHCHVREELNYSGN
                     FVAQAGWAWRGPYSSHLIRAGLHYYNGLSDQYSFFRNFEQQIGIGFWYDF"
     gene            4218..5585
                     /locus_tag="VFB80_03635"
     CDS             4218..5585
                     /locus_tag="VFB80_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN32878.1"
                     /translation="MSLRPLCLALVSFVSFVPCLAEEPVTPKPSATKPTDDAQLLRLF
                     ADTLDQVERNYVKPVDRRELLEAAIRGMLSKLDPHSTYIPPADLDRFKTSVENEFGGI
                     GVTVSAADGVLTIVTPLLGTPAYKAGLRGGDTIVEINGESAAGISVDDAVLRVKGPVG
                     TSVKLKIKHAASSETEVVEVGREIVRVESVLGDRRKPDDTWNFFLDEEKKIGYLRIVN
                     FGRHTADELRSALGDLKRSGMKGLILDLRYNPGGLLTAAIEVSDLFVSQGRIVSTAGR
                     TGQQRKWDAHESGTFEGFPMVVLVNRLSASASEIVAACLQDHRRAAIAGQRTWGKGSV
                     QNVIELEGGQSALKLTTGGYLRPSGQNIHRFEGAAEKDDWGVRPDDGLEIVLSADESE
                     AYQTSRRKRDAIVAHPDNAPSPAADKLDKQLQAALDHLQKLIDKDSAQPADNKTAQKS
                     PAEQP"
     gene            5601..>5753
                     /locus_tag="VFB80_03640"
     CDS             5601..>5753
                     /locus_tag="VFB80_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZN32879.1"
                     /translation="MRILTIETTCDETAAAVVADDLTVLSSVVASQDELHRRFAGVVP
                     EIAARAH"
BASE COUNT          991 a         1935 c         1844 g          983 t
ORIGIN      
        1 tccgcggcac cacgccgtct tgcgtgcaat agctggggct cagccggccg accgccccga
       61 acgcctgctt gcggcacttc cagagcttgg cccgctgctt ggggtcgttg gcctggcgga
      121 cttcgcgggc gccgcacttg ctccgcgttc cgctcgtcga atagcaaaat cgggtggata
      181 ttgccgtcgc cggcgtgaaa gacgttgacg atccggatgt cgtagcggcg gccgatctcg
      241 atgatctttc gcaggatgtg cggcagctgt gtccgcggca ccacgccgtc ttgcgtgcaa
      301 tagctggggc tgagccggcc gatcgccccg aacgcttgct tgcggctctt ccagagcttg
      361 agccgttctt tttcatcccc ggccagccgc acttcgcgag cgccgcattt ctggcataac
      421 gcgataatct gatcgcgctg ctggtcgagg ccggcctcca gcccgtccac ttcgatgagc
      481 aggatcgcct gggcgtccag cggaaagcca aacccgaagg ccgcttccac cgcgaccagg
      541 atgccctgat ccatcatctc cagcgccgcc ggcacgattc ccgcgccgat gatttcgctg
      601 atcgccgccg tggcgtcatc gaccgattcg aagacgccga gcatcgtgcg gtggccttcc
      661 gggctgcggg tcagtcggac ccagactttg gtgacgatcg ccagcgtccc ttcgctgccg
      721 acaatcgccc cgacgaggtc cagccccagc ggatcgccgg ccggcccgcc gagccgcacc
      781 acgcggccat cggccagcac ggcttccacg cccagcacgt gattgaccgt cacgccgtac
      841 tttagcgtgt gcggcccgcc ggaattcgtc gccacgttgc cgccgatcgt gcaggccccc
      901 tggctcgaag gatcaggcgc gtagtgatag ccagtgccct tgagggcatt ggtcagccag
      961 acgttgacga cgcccggctc gacgacggcg tagcggtcgc ggagattaat ctccagaatc
     1021 cgcttcatcc gtgtcaggac gatcatcacg ccgccgccga ccggcaggca gccgccggcc
     1081 aggctcgtgc ccgccccccg cggcagaaac ggcacgccca actcgctagc gatcttcacg
     1141 acccgcgcga catcggccgt cgagcgcgga aacacgacga catccgggct gttcttctcg
     1201 atcgtgaagc cgtcgcactc atagaccagc aggtcggact gtgccgagag cacgttctct
     1261 acgcccaccg cttcgcggag gcgactgacg agcggcggca gggcgagggc gggcatgagg
     1321 cggttgggcc gatgcgcgaa gtggaggtcc aattatattt cgccgcgctg ctggaggaag
     1381 ccggcggacg atgcctatcc caatcgctcg gccagtttct ccaccaccag cttgccgata
     1441 ttcagcgcgg aagtggcggc gggcgagggg gcattgagga cgttgacaat tcgcccgcgg
     1501 ggctggatga caaagtcatc gaccattgcg ccgtccggtg cgacggccat cgcccgaact
     1561 ccggatcgcg ccggcacaag gtcctcgctc gtgatttccg gaacgagatg ccgcagtgcc
     1621 cggacgaacg ccgatttgct gagcgagcgc cacatctcgc cggccccggt ccgccagtat
     1681 ttccaggcca gcttacgaaa gccgggaaag gccagcgtct cgcagaggtc ccccaggttc
     1741 acgtcgctct tgcggtagcc ctcgcgggcg aaggccagca cggcattggg cccgcactcc
     1801 actcccccct cgatcatccg cgtgaagtgc actcccaaaa acgggaagct cggatcggga
     1861 accggataga tcaggttgcg gcacaggtgc tgcgactcgt gcttcagctc aaaatactcg
     1921 ccgcgaaagg gcacgatctg cgcttccggc tgaatgcccg tgagcttcgc cacgcggtcg
     1981 ctgtgcaggc cggcacaggt cacgacgtaa tccgccgcaa actcgccagc ggtggactcg
     2041 agcgcgatct ggtcgccgtt ttccttcacg gccagcacgc gagccaagca gcggacttcg
     2101 ccacccccga cggtgatcag ctccgccagc ttgcggctga cagccgcgta attgacgatc
     2161 cctgcctcgg ggacgtggat cgccttcacg cccggcgcgt gcggctccag ttccttcaac
     2221 cgctcgggac ctacaagctc gcagcgaatg ccgttctgct gaccgcggtc atagattttc
     2281 tcgagctgcg gcagctccga ctggctcgtc gcgacgatga ccttgccgca aatctcgaac
     2341 ggcacgccgt gcgccgcgca aaactcctcc atcgcccgct tgccgctgcg gcagttgacc
     2401 gcccgcagcg agcccggctt gtagtagatg cccgaatgca atacgcccga attgtgcccc
     2461 gtctggtgag cggcgaccgt gggctctttg tcgagcacca ggggccgcag ccgcggatag
     2521 cgctgcaaca gctgccaggc ggtcgccagg ccgacgattc cgccgccgac gatggccaca
     2581 tcgaagcgat tggcggtcat tcgactttct tcagctcgcg gccgagatgg gcttcgacgc
     2641 tggcgacctt ggcgctgagc cggccggaat gcccgcggcg aaagtcgagc ttggcggagc
     2701 aagtgatccg gtcgcagtcg gccgccatcg cttccaggca ctgcttgagg acgcccagca
     2761 cgtcgtcgat ctccccttcc agaatcgtcc ccattgcgtg caggcgatag tcgagcccgc
     2821 tccgctcgat gatttccagg ctgcgggcca cgtagttgcc gacactcggc cccttttcca
     2881 gcggcgccat gctgaattcg agaagcacca tcggttgagc cctccctaaa gctttggcat
     2941 gacgagagtt gcgcccgggc ggattggcct acgtcgggcc ttgccggctg ggtaaacttc
     3001 gccgcctgtc ggcattatgc ccgctggccc ggttatagcg ggaaatcgca aggctccgaa
     3061 gacgccgagc gggcatggtc gaaactcctt gagaaggtgc gggttatccg gcctctagtg
     3121 atagtacgcc ccatgcgcgt ggagcgatgg ttgccggttg tgctggcggc gctggtcgcc
     3181 tggtcgcccc cggctcgcgc gcaactggaa tcgccgattg cgccgggatt cgaaccgctg
     3241 cccgctggcg agaccattgc acccggccac atcgagccga ccggaccgcc cctggactac
     3301 ttcgatctcg atgcctgggc agccacgcag agcggaccga ccgtcccgca gggcgactgg
     3361 tcgtggcaaa tcctgccgga cgggatcatc taccgggcct acctcgccaa tcccaaggag
     3421 tcgcggatcg gcacgacgat tttttgggag accgacgagg ggcagtggtg ggactcgacg
     3481 ctgggcggca gggcgggcat tctgcgctac ggcacgcgcg atacggcctg gccgcagggt
     3541 tggcagctgg acatcgaagg ttccgtccag gtgcggctgg atcccgaaga ggaccgcgac
     3601 ttgcggagtt ccgactaccg attcggggtg ccgctgacct acggctacgg ccgccatcgc
     3661 ctcaagatcg gctactacca ccactgctcg catctgggcg acgagttcct ggaggaccat
     3721 ccgacctttc tccacgtcaa cttcgtccgc gacgtgttca cgctcggcta cgcgtacttc
     3781 gtgaccgaca acctgcggct ctacggcgag atcggctacg gattctacgt cgatgtgagc
     3841 aaggagtggg agctgcagtt cggcctggaa tatgctccgg ccctgccgac cggtcttcgc
     3901 ggcgcgcctt ttttcgcggt ccactgccac gtgcgtgaag agctgaacta cagcggcaac
     3961 ttcgtcgcgc aggcgggctg ggcctggcgc gggccctaca gcagccatct aatccgcgcc
     4021 ggcttgcact attacaacgg cctctccgac caatactcat tcttccgcaa cttcgagcag
     4081 caaatcggaa ttggcttctg gtacgacttc taagggttgc ccgcaacttt cgttgttccg
     4141 cgcgggcggc aatcgtaaga tgagttcggc agccgccgct tcggccggcc gccgccacct
     4201 tccccgccag gcttgccatg tcgctccgcc cgctctgtct tgcactcgtc tcattcgtct
     4261 cctttgtccc ctgcctggcc gaagaacccg tcaccccaaa gccgtccgcc accaagccca
     4321 ccgacgacgc ccagctgctg cggctgtttg ctgacacgct cgaccaggtg gagcggaact
     4381 acgtgaagcc ggtcgaccgc cgcgagcttt tggaggcggc gattcgcggc atgctatcga
     4441 agctcgaccc gcactcgact tacatcccgc cggcggacct ggaccgcttc aagaccagcg
     4501 ttgaaaacga gttcggcggc atcggcgtta cggtctcggc ggcggacggc gtgctgacga
     4561 tcgtgacgcc gctgctgggc acgccggcct acaaggcggg cctgcggggc ggcgatacga
     4621 tcgtggagat caatggcgag tcggcggccg gaatctccgt cgatgacgcg gtgctgcggg
     4681 tgaaggggcc cgtgggcacg agcgtcaagc tgaagatcaa gcacgcggcc agcagcgaaa
     4741 cggaagtggt ggaggtcggc cgcgagatcg tccgcgtcga atcggtgctg ggcgaccggc
     4801 ggaaacccga cgacacctgg aacttctttc tggacgagga aaaaaagatc ggctacctcc
     4861 gcatcgtgaa tttcggccgc cacaccgccg acgaattgcg ctcggcattg ggcgacctga
     4921 agcgcagcgg gatgaaaggg ttgattctcg atttgcggta caaccccggc gggctgctca
     4981 cggcggccat cgaggtcagc gacctgttcg tgtcgcaagg acggatcgtc agcaccgccg
     5041 gccgcaccgg gcaacagcgc aagtgggacg cccacgagag cggcacgttt gagggctttc
     5101 ccatggtcgt gctggtcaac cgcttgagcg ccagcgccag cgagatcgtc gcggcctgcc
     5161 tccaggacca ccgccgggcg gcgatcgccg gccagcggac gtggggcaag gggagcgtgc
     5221 agaacgtgat cgagctcgaa ggcgggcaga gcgcgctcaa gctcactacc ggcggatact
     5281 tgcggcccag cgggcagaat atccatcgct tcgaaggcgc tgctgagaag gacgactggg
     5341 gcgtgcgtcc ggatgacggg ctggagatcg tcctgtcggc cgacgaaagc gaagcctacc
     5401 agacatcgcg ccgcaagcgg gacgcgatcg tggctcatcc ggacaatgcc ccttcgcccg
     5461 cggccgacaa gctcgacaag cagctccagg cggcgctcga ccacctgcag aaactgatcg
     5521 acaaggattc tgcgcagccc gccgacaaca agactgccca gaagtcgccc gcggagcagc
     5581 cgtagcggcg gatcggctcg atgagaatcc tgacgatcga aaccacgtgc gacgagacgg
     5641 cagcggccgt cgtcgcggat gacctgaccg tgctgtcgtc ggtcgtggcg tcgcaggacg
     5701 aactgcaccg ccggtttgcc ggcgtcgtgc cggagatcgc ggcccgcgcg cac
//
LOCUS       DASRJB010000125        10108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3703536, whole genome shotgun sequence.
ACCESSION   DASRJB010000125 DASRJB010000000
VERSION     DASRJB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703536"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFB80_03645"
     CDS             complement(<1..736)
                     /locus_tag="VFB80_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32880.1"
                     /translation="MPTLSELPPHYRLIVVLSPIWLLNWLILAAVDPDGPGSGRGWEY
                     VILGNLIGSMFAHGTLAAAWCAWGPGWLAARLPLSLAWIAASLGAVAINIGINGGPGD
                     AVPILAACMVAQWLLVQVPLWCIALGYGLQIRATDSRYSTPSRQDLQFGIRQLMILTA
                     IVAVMLGLARFLVMWLGARWSNWGGDTVAFIFLAAAAVIMTLPLLLAALLPRRALPAT
                     LVVLLLIAGATAAESPLFHRLPQAGGG"
     gene            830..1150
                     /locus_tag="VFB80_03650"
     CDS             830..1150
                     /locus_tag="VFB80_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32881.1"
                     /translation="MQPHALPMSEASCQRVPELLRSLGFSVEPHHDINEFKMLRCARE
                     GVGVLLSMNKPAHWAAASDERKNLVMVAAIGESMFRFWRIPRESRIAREVIDLLRPLE
                     WRPH"
     gene            complement(1147..2751)
                     /locus_tag="VFB80_03655"
     CDS             complement(1147..2751)
                     /locus_tag="VFB80_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN32882.1"
                     /translation="MAALWAQEKSAPPLFDGLGDFGRKITTTSPQAQRYFNQGLCWLY
                     AFNHDEAIRSFHHATTLDPQAAMPWWGIAVASGPHINNPVVSTEKAKSAWTALAKAKE
                     RSAGASKIERALIEALASRYADPQPDDRKPLDEAYATAMRKVWQANPTDADVGALFAE
                     SLMDLRPWDQWQPAGKPQPGTEEVVNTLQAVLTQTPNHPLALHLYIHAVEASPHPELA
                     AAPADRLRNLQPGLGHLVHMPSHIDVRLGHWAAAIEANRKAIEADGKYREQVPQQYFY
                     RVYIAHNHHMLAFAAMMRGQSRLAIDEIDAMVKGIPPQWLKDNATAADGFASMPLEVL
                     VRFGRWDEVLAAPEPPEHLPLSRALRHVSRGIAFAAKGQSDTARAEQQAFLAARKLVP
                     GNVTFGNNKAAELMNVAEKLLAGEILWRDGKNDAAFSALREAIKLEDQLRYSEPPDWI
                     HPVRHALGATLLAAGRAAEAEAVYREDLAKLPSNGWSLYGLAECLKQQGKLAESRELE
                     ARFREVWKDADIRITSSCYCQPGKQN"
     gene            complement(2843..4243)
                     /locus_tag="VFB80_03660"
     CDS             complement(2843..4243)
                     /locus_tag="VFB80_03660"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HZN32883.1"
                     /translation="MGLAVIAGAAGAADADPALREQAAAAMRKAAEFYRGQVATRGGY
                     VYYYSPDLTKRLGEGVAGPTQIFVQPPGTPTVGLAYVRAYEATGDRFYLEAARETATA
                     LIYGQLKSGGWTQTVDFDPRGTKVALYRNGQGRGANNSTLDDNITQSAIRFLMHLDRA
                     TQFKDPQVHEAVSIALDALLKAQFPCGAFPQVWTGPVLPQPLARGNFPTHDWRTEGKI
                     KEYWNMYTLNDGLAGTVSATLLDAFEIYKDDKYKAAAAKLGDFLIAAQIPDPQPAWAQ
                     QYNYAMQPIWARRFEPPAITGGESQDVLETLLKIYRATGDAKYLEPIPRALAYLKRSH
                     LEDGRLARYYELQTNKPLYMSRRGDEYTLTYSDADLPDHYGWKVASRLEAIEADFAAA
                     KAGRTTQKPPTSAELADKARRIIADLDSQGRWISAYAGEPLYGQPKFKMGEQYLHSGV
                     FAANVETLSACLKATR"
     gene            4416..5117
                     /locus_tag="VFB80_03665"
     CDS             4416..5117
                     /locus_tag="VFB80_03665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZN32884.1"
                     /translation="MLLRNRKLQGLVGGTFFEWLGIDIVARLEMFALAFHKDWTKLKV
                     LRRARRERWSMVTNQEMFIIQSIASGMVKVPGEYAEVGIYQGSTAKVICEAKGDKPLH
                     LCDTFEGLPEPGEHERQVEKKGRFACSLESIQKYLGSYPNLHYHKGYCPQSVEGVLDD
                     KRFAFVHLDVDLYASTKQCLEYFWPRLNPGGIVISHDYSILEGVRRAFTEFTATTREQ
                     VIELPTTQCMMVKAG"
     gene            complement(5122..5619)
                     /locus_tag="VFB80_03670"
     CDS             complement(5122..5619)
                     /locus_tag="VFB80_03670"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZN32885.1"
                     /translation="MQQPTIAAKPPLTVVGMERSFIHSQSPDANNLQVLGELWREFPP
                     RAREIPNRVGHAMYGVIYERPPAERKHRHELQYIAGVAVSAGDHIPAGLVARTIPGAT
                     FAVFLHRGPINQIAATVREIYREWLPASPYQHAGIADIELYDDRFCADGPDSEMEYWI
                     SIQPK"
     gene            complement(5698..8580)
                     /locus_tag="VFB80_03675"
     CDS             complement(5698..8580)
                     /locus_tag="VFB80_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HZN32886.1"
                     /translation="METLETRAVPTSGLLYSNMAQEIMEQAPKLHPGTAYYLQFMRSD
                     GSFRDLNYSGKSDASAGDLQGHGKRLETLTLSLKWNDPSNPYYNSPWLQSQILKGWNY
                     VAYKGGSVNAPNWWWKAIGVPQGMAQGLILMRDEMSPAVRSQVLKKYFGSVWSPSKYD
                     GANVTSQAPLAMIDGLLRGNTSRIREVVNRVSAELNAYGGEGIQRDLSFQQHRLNGKL
                     NYYSGHYGLLLAKDSAQIMRWTGGTDFAFGGAAIHQELRFLLDHLQWLTRGDTFDVAS
                     MGRIATSHSALSNAAGTLNDALVDMLTLGSRTDELTSAIERYTLGSSDANSLSGSKAL
                     WRSDGMAWQRPNMLATLRMLSNRTLRPETAAGQNARGFFEGDGFTLLVQDGDELGGSG
                     ESSILPVWDWQRLPGTTVQHNGWIPNLDMFKTSTNSTGGSNLVGSASDGNYGVALMDY
                     RRGGVSLTARKSWFFFDDEIVALGSDIDDAQASAPVFTSLNQVLLDGPVTVQDAAGRR
                     TFELGGSASLSGGAWIHHDDLGYVVLDASSRTTVQAQMQWGGGLSLPVFSAWVDHGAR
                     PQNQTYAYAVVPGVSPDELDAYTGQLPIQILNNTPTIQAVRHLGLNQTQIAFYSAGAT
                     MIDDTTWIAVSQPALVIVRQVGDDLVITASDPRQQATSLTIDVNRQLIGPGAKWQPDA
                     QTTRITMALPALPYQGASATATYFGLPADPIDEPAPVNAVAVPAQADAAVRTGSYADT
                     PDGNSAELAIQNFTTGYTRESFVRFDTSAIVGEIVSAEVRLTAVSGANPVLISAAPTL
                     DDAWQESSITWNSKPASGPEIGRASVSQGQVAAFDVTSLVREAVGSDDLFSLRLASPT
                     QGYYLVGFASREHADVALRPVLEVITRPSTIESSASAEGESRTGLSLTLGDYLSLATS
                     TSSAGPVLGPTMAGPSTSDRRMLRIGAASDGDVWDPSVVDLALGQY"
     gene            complement(8854..9021)
                     /locus_tag="VFB80_03680"
     CDS             complement(8854..9021)
                     /locus_tag="VFB80_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32887.1"
                     /translation="MDGKAKKRLEVIQPKLQLLRQKLAGAKKQNDEPGEVRKLEAEIA
                     ALEAEVAKLKA"
     gene            complement(9041..9727)
                     /locus_tag="VFB80_03685"
     CDS             complement(9041..9727)
                     /locus_tag="VFB80_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32888.1"
                     /translation="MNERAQAIEVLREAKAVLAVRLTEKVLEQAEDILADARGDSYMN
                     EIDTVYEQVGLKLAHVSQMLANLPPEADTSRGSVPGEGDTFTVATEPAPSADAVIENT
                     IPALTGPLYVAAPALPAPQTRTTGSSRVASAALQAFAAQIQAGDLLAAGRTLAALFEI
                     EETRGVACAATFAQRVRHEAAFFRKVMELRGELHSANPQRALLLLLDCFGLVRGEAAE
                     VLRNLQRRRT"
     gene            complement(9827..>10108)
                     /locus_tag="VFB80_03690"
     CDS             complement(9827..>10108)
                     /locus_tag="VFB80_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32889.1"
                     /translation="QMIVGIKFLLSLPIFFIASVLAGRSDLAKRFQFSAKFWMNVNLT
                     LAVLMVLIGGGLRFVGRTLKAPATPPAPAAAIVPQDRPNASLRLRGQIE"
BASE COUNT         1676 a         3252 c         3362 g         1818 t
ORIGIN      
        1 cgccgccgcc ggcctgaggc aaccggtgaa acaagggact ttcggccgcg gtagccccgg
       61 cgatcagcag cagcacgacc agcgtcgccg gcaaggcccg ccgcggcagc agggcggcga
      121 gcaacagggg cagcgtcatg atgactgccg ccgccgccag aaagatgaag gcgacggtgt
      181 cgccgcccca attggaccag cgagcgccga gccacatcac caggaacctc gccagcccca
      241 acatcacggc cacgatggct gtcaggatca tgagctggcg gatgccaaac tgcagatcct
      301 gccgtgacgg cgtcgagtag cgcgaatcag tcgcccggat ctgcaagccg tagcccagcg
      361 cgatgcacca caagggaact tgtactagca gccactgcgc caccatgcag gcggccagaa
      421 tgggaacggc gtcgcctggc ccgccgttga tgccgatgtt gatcgccacc gcgcccaggg
      481 aggccgcaat ccaagccagc gacagcggca agcgggccgc cagccacccc ggtccccacg
      541 cgcaccaggc tgccgccaag gtgccatgag cgaacatcga gccaatcagg tttcccagga
      601 tgacgtattc ccagccgcgg cccgaaccgg gtccatccgg atcgaccgcc gccaggatca
      661 gccagttcag cagccagatc gggctgagaa ccacgatcag ccgatagtgc ggcggcaatt
      721 ctgacagggt tggcataagg cacacggccg gagcgggcgc taggggcttg ttcgccgccg
      781 cacggccgat ctttcaagat ccccgtgacg cacggcctat atttcaacca tgcagcccca
      841 tgccctgccg atgtccgaag cgtcctgcca gcgagtgccg gagttgctcc gctcactcgg
      901 gtttagtgtg gagccgcatc acgacatcaa cgaattcaaa atgctccgct gcgcccgcga
      961 gggcgttggc gtcttgctct ccatgaacaa gccggcccac tgggccgccg cctccgacga
     1021 gcgcaagaac ctggtcatgg tcgcggccat cggcgaatcg atgtttcgct tctggcgaat
     1081 tccccgcgag agccgcatcg ctcgcgaagt gatcgacctc ttgcggccgc tggagtggcg
     1141 gccgcactag ttctgcttgc ccggctggca atagcacgac gaagtgatcc ggatgtcggc
     1201 gtccttccag acttcgcgga agcgggcctc cagttcgcgc gactcggcga gcttgccttg
     1261 ctgcttgaga cactcggcca ggccgtacag cgaccagccg ttgctcggca gcttcgccag
     1321 gtcctcgcga tagaccgcct cggcttcggc cgcgcggccc gcggccagca gcgtcgcccc
     1381 cagcgcgtgg cggacgggat gaatccaatc cggcggctcc gaataccgca gctgatcctc
     1441 cagcttgatc gcctcgcgca gggcggagaa cgctgcgtcg ttcttcccgt cgcgccacag
     1501 gatttcgccg gccagtagct tctcggcgac attcatcagt tcggcggcct tgttgttgcc
     1561 gaaggtgacg ttgcccggca cgagcttgcg ggcggcgagg aaggcctgct gctcggcgcg
     1621 ggccgtgtcg ctctggccct tggcggcgaa ggcgatgccg cgcgagacgt gccgcagcgc
     1681 ccgcgacagc ggcagatgtt ccggcggctc tggggccgcc agcacttcgt cccagcggcc
     1741 gaaccgcacg agcacttcca gcggcattga ggcaaagccg tcggcagccg tggcgttgtc
     1801 cttcagccac tgcggcggga tgccctttac catcgcgtcg atttcgtcga tggccagccg
     1861 gctctggccg cgcatcatcg ccgcgaaggc cagcatgtgg tggttatggg cgatgtagac
     1921 gcgatagaag tattgctgcg gcacctgctc gcggtacttg ccgtcggcct cgatcgcctt
     1981 gcgattcgcc tcgattgccg cggcccaatg tcccagccgc acgtcgatgt gcgacggcat
     2041 gtgaaccagg tggcccaggc ccggctgcag gtttctcaag cggtcggccg gggcggcagc
     2101 caattccgga tgcggcgaag cctcaaccgc gtggatatag aggtgcagag ccagcgggtg
     2161 attaggtgtc tgagtgagta cggcctggag cgtgttgacg acctcctcgg ttcccggctg
     2221 cggtttgccg gctggctgcc actggtccca cggccggagg tccatgagcg attcggcgaa
     2281 cagcgctccg acgtcggcgt cggtcgggtt cgcctgccag actttccgca tcgcggtcgc
     2341 gtaggcttcg tcgagcggct ttcggtcgtc gggctgcgga tcggcatagc ggctggccag
     2401 ggcttcgatc agggcccgct cgattttgct cgcgccggcc gatcgctctt tcgccttggc
     2461 gagtgcggtc caggcgctct tggccttctc ggtcgagacg accgggttgt tgatgtgcgg
     2521 cccgctggct acggcgattc cccaccaggg catcgccgcc tgcggatcga gcgtggtcgc
     2581 gtgatggaac gaccggatcg cctcgtcgtg gttgaaggca tacagccagc acaggccctg
     2641 gttgaaatac cgctgcgcct gcggcgaggt agtcgtgatc ttccggccaa aatcgcccag
     2701 accgtcaaac agcggcgggg ccgacttctc ttgtgcccaa agtgcggcca atggccacga
     2761 gaagcacaaa aagcacaaga gagctcttaa atgagatttc gcccacattc ttttgcgcct
     2821 cttcgtgttt ttcgtggcaa tcctaccgag tcgccttcaa gcaagcgctc aacgtctcaa
     2881 cgttcgcggc gaacaccccg ctgtgcaagt attgctcgcc catcttgaac ttcggctgac
     2941 cgtacagcgg ctcgccggca tacgcgctga tccagcggcc ctggctgtcc aggtcggcaa
     3001 taatccgccg ggccttgtct gccaattccg cactcgtcgg cggcttctga gtcgtgcggc
     3061 ccgccttggc cgcggcgaaa tctgcctcga tcgcctcgag ccgcgaggcg accttccagc
     3121 cgtagtggtc gggcaggtcg gcgtcgctgt aggtcagcgt atactcgtcg ccgcggcgtg
     3181 acatgtagag cggcttgttc gtttgcagct cgtagtagcg ggccagcctt ccgtcctcca
     3241 agtgcgagcg tttcaaatac gccagggccc gcgggatcgg ttccaggtat ttggcgtcgc
     3301 ccgtcgcccg gtaaatcttc agcagtgtct cgagcacgtc ctgcgactcg ccgcccgtga
     3361 tcgccggcgg ctcgaaccgc cgggcccaga tcggctgcat cgcatagttg tattgctggg
     3421 cccatgccgg ctgcggatcg ggaatctggg cggcgatcag aaaatcgccc agcttcgccg
     3481 cggcagcctt gtacttgtcg tccttgtaga tttcgaaggc gtccagcagc gtcgccgaaa
     3541 ccgtgcccgc caggccgtcg ttcagcgtgt acatgttcca atattccttg atcttccctt
     3601 ccgtccgcca gtcgtgcgtt gggaagttgc cgcgggctag gggctgcggc agcacgggcc
     3661 cggtccagac ttgcggaaac gcgccgcacg ggaattgcgc cttgaggagc gcatccaggg
     3721 caatgctgac ggcctcgtga acctgtgggt ctttgaactg cgttgcccgg tcaaggtgca
     3781 tcaagaagcg gatcgccgac tgggtgatat tgtcgtccag cgtcgagttg ttcgcgccgc
     3841 ggccttgccc gttgcggtag agcgcgacct tcgtgccccg cggatcgaaa tcgacggtct
     3901 gcgtccagcc gccggacttg agctggccgt aaatcagcgc cgtggccgtc tcgcgggcgg
     3961 cctcgagata aaaccggtcg ccggtcgcct catacgctcg cacgtacgcc aggccgaccg
     4021 tcggcgtccc cggcggctgc acgaaaatct gagtcggacc ggcgactccc tcgcccagcc
     4081 gcttcgtcaa atcgggcgaa tagtaataga cgtagccgcc gcgggtggcg acctggccac
     4141 ggtaaaattc ggctgctttt cgcatggccg ccgcggcctg ctcgcggagg gcgggatctg
     4201 cgtcggccgc gcccgccgcg ccggcaatta cggcgagacc caaccaggcg aaatgccgat
     4261 atgtctggaa aatggtggtt tctcctactc aacggcgagt gagccaacca gaatggttcg
     4321 tcggacccgc ttagccccgg ccgccaggcc gggcgtcagt cgaagtgcaa ccggcctggg
     4381 ggccggggct aagcgaggtc aaaaggaaac gattcatgct gctccgcaat cgcaagctcc
     4441 aaggcctggt cggcgggaca ttcttcgaat ggctggggat cgatattgta gcccggctgg
     4501 agatgttcgc gctggcgttt cacaaggact ggaccaagct caaggtcctg cggcgggccc
     4561 gccgcgagcg gtggtcgatg gtcaccaacc aggagatgtt catcatccaa tcgatcgcca
     4621 gcggcatggt caaggtgccc ggcgagtacg cggaggtcgg catttaccag gggagcacgg
     4681 ccaaggtgat ctgcgaggcc aagggggaca agcccttgca cctgtgcgac acgttcgaag
     4741 ggctgcccga gccgggcgag cacgagcggc aggtcgagaa gaagggccgc ttcgcctgca
     4801 gcctggagtc gattcagaaa tacctcggct cgtatccgaa cctgcactac cacaaaggct
     4861 actgcccgca gtcggtcgag ggcgtgctcg acgacaaacg attcgccttc gtccatctcg
     4921 acgtcgatct ctacgccagc accaagcagt gcctggagta tttctggccg cggctgaatc
     4981 ccggcggcat cgtgatctcg cacgactatt ccatcctgga aggggtccgc cgggccttca
     5041 ccgagttcac cgccacgacc cgcgagcagg tcatcgagct gcccacgacg cagtgcatga
     5101 tggtcaaggc ggggtgagcc gctacttggg ctggatggag atccagtact ccatctcgga
     5161 gtcggggccg tccgcgcaga agcggtcgtc gtagagctcg atgtcggcaa ttcccgcatg
     5221 ctggtagggc gaggccggca gccactcgcg gtagatttcg cgaaccgtgg cggcgatctg
     5281 gttgatgggt ccgcggtgca ggaagacggc gaacgtcgcg ccagggatcg tgcgggccac
     5341 caggccggcc gggatgtgat cgccggcgct gacggcgacg cccgcgatgt actggagctc
     5401 gtggcggtgc tttcgctcgg ccggcggccg ctcgtaaatg acgccgtaca tcgcgtggcc
     5461 cacgcgattt ggaatctcgc gggctcgcgg agggaactcg cgccacagct cgcccagcac
     5521 ctgcaggttg ttggcgtcgg gcgactgcga gtgaatgaag gaccgctcca tccccacgac
     5581 ggtcagcggc ggcttggcgg caatggtcgg ctgctgcatg tccatacctc cggtcgcgac
     5641 cggagtcatt ctaacagggc agcgctacct caagatgtcc ctcgctgacg ctttgcgtta
     5701 gtattggccc agcgccagat cgaccaccga tggatcccaa acatcgccgt cgctggcagc
     5761 gccgatccgc agcatgcgcc ggtcgctggt tgacggtcca gccatcgtgg gaccaagaac
     5821 cgggcccgcg gagctcgtgc tggtggccag gctgagatag tcacccaagg tcagcgacag
     5881 tccggtccgc gattcccctt cggccgaagc ggacgattcg atcgtgctgg gccgcgtgat
     5941 gacctccagc accggccgca acgcgacatc ggcatgctcc cgcgaggcaa agccaaccag
     6001 gtaatagccc tgcgttgggc tcgccagccg tagcgagaac agatcgtcgc tgcccacggc
     6061 ctcgcggacg aggctggtca cgtcgaacgc ggccacctgc ccctggctga cgctcgcgcg
     6121 gccgatctcg ggcccgctgg cgggcttgct gttccaggtg atgctcgact cctgccatgc
     6181 gtcgtcgagc gtgggagccg cggagatcag gacgggattc gccccgctga ccgccgtcag
     6241 ccgcacttcg gccgagacga tttcgcccac gattgcgctg gtgtcgaacc gcacgaagct
     6301 ctcgcgggtg tagccggttg tgaaattctg gatggcgagc tccgcggaat tgccgtccgg
     6361 ggtgtcggca tagctgccgg tgcgaaccgc ggcgtcggcc tgggcgggaa cggccaccgc
     6421 attgaccggc gcgggttcgt ctatgggatc ggcaggcagg ccgaagtagg ttgccgtcgc
     6481 gctggcgcct tggtacggca aggcgggcag ggccatcgtg attcgcgtcg tttgcgcgtc
     6541 cggttgccac ttcgctccgg ggccaatcaa ctggcgattc acgtcgatcg tgagactggt
     6601 ggcctgctgc cggggatcgg aggcggtgat caccaggtcg tcgccgacct ggcggacgat
     6661 caccagcgcg ggctggctga cggcgatcca cgtcgtgtcg tcgatcatcg tcgcgccggc
     6721 ggaatagaac gcgatctgcg tctgattcag gcccaagtgc cgtacggcct ggatcgtcgg
     6781 cgtgttgttc agaatctgga tgggcagctg gccggtgtag gcgtccagct cgtccggcga
     6841 gacgcccggc acgacggcat aggcatacgt ctggttctgc ggccgagcgc cgtgatcgac
     6901 ccaggcgctg aacaccggca gcgacaggcc gccgccccac tgcatctggg cctggacggt
     6961 cgtgcggctg ctggcgtcca aaacgacgta gcccaggtcg tcgtggtgga tccacgcgcc
     7021 gccgctgagc gaggcagagc caccgagctc gaacgtccgc cggccggcgg cgtcctgcac
     7081 ggtgaccggg ccatcgagta gcacctggtt gagcgacgtg aaaacgggcg cggaggcctg
     7141 ggcgtcgtcg atgtccgagc ccagggcaac gatctcgtcg tcgaagaaga accacgactt
     7201 gcgggccgtt agcgagacgc cgccgcggcg atagtccatc agggccacgc cgtagttgcc
     7261 gtcgctggcg ctgcccacga ggttgctgcc ccccgtggag ttggtcgagg tcttgaacat
     7321 gtcgaggttc ggaatccagc cattatgctg cacggtcgtt cccggcaagc gctgccaatc
     7381 ccagaccggg aggatgctgc tctcgccgct cccacccagc tcgtcgccgt cctgcacgag
     7441 cagcgtgaag ccgtctccct cgaagaaccc gcgggcgttc tgcccggccg ccgtttcggg
     7501 ccgcagcgtg cgattcgaga gcatccgcag cgtggccagc atgttgggcc gctgccaggc
     7561 catgccgtcg gaccgccaca gcgccttgct gcccgacagg gaattggcgt cgctcgagcc
     7621 cagcgtgtag cgctcgatcg cggaggtcag ctcatcggtc cggctgccca gcgtcagcat
     7681 atcgacgagg gcgtcgttca gcgtgccggc cgcgttgctc aaggcgctat gggaagtggc
     7741 gatgcggccc atcgaggcca cgtcgaacgt gtcgccgcgg gtcagccact gcaaatggtc
     7801 gagcaggaac cgcagctcct gatggatggc cgctccgcca aaggcgaagt cggtgccgcc
     7861 ggtccagcgc atgatctgcg ccgagtcctt ggcgagcagg agcccgtaat ggccgctgta
     7921 gtagttcaac ttgccgttca gccgatgctg ctgaaacgag agatcgcgct gaatgccttc
     7981 gccgccgtag gcattgagct cggccgagac gcgattgacg acctcccgga tgcggctcgt
     8041 attgccgcgc aagagtccgt cgatcatcgc cagcggcgct tgagaggtca cgttcgctcc
     8101 gtcgtacttc gacggcgacc agaccgagcc gaagtacttt ttcaggactt gcgagcggac
     8161 ggccgggctc atctcgtcgc gcatcaggat cagcccctgg gccatcccct gcggcacgcc
     8221 gatcgccttc caccaccagt tgggtgcgtt gaccgacccg cctttgtagg cgacatagtt
     8281 ccagcccttc aagatttgcg actgcagcca ggggctgttg tagtagggat tgctcgggtc
     8341 attccacttc agcgagagag tcagcgtctc cagccgcttg ccgtggcctt gaaggtcgcc
     8401 cgcgctggcg tcggacttgc cgctgtagtt gaggtcccga aagctgccgt cactccgcat
     8461 gaattggagg taataggcag tccccgggtg gagcttcggg gcctgctcca tgatctcctg
     8521 ggccatgttg ctgtaaagca atcctgacgt cggcaccgct cgcgtttcga gcgtttccat
     8581 ggtcagctgc cgcggcttgc gcggttgctg caacttatgg tgcggattgg acttgcgatc
     8641 agatacagtg gaacgagtcg ggggaaaccc aatcacagtt gcggctcatg gtgcggttgg
     8701 cgatcatcct tggtgaccct tcttcctggt gagtgccaag ttaatcaacc tgaacacgag
     8761 ccgctagact gcgggcttta gaaaagccag agaaaccggg gtgaatagtc cggtttggag
     8821 aactttaacg gagacgaccg ccaacagtgg gcgtcaggcc ttcagcttcg ccacttcggc
     8881 ctccagcgcg gcgatttcgg cttcgagctt gcggacttcg cccggctcgt cattctgctt
     8941 cttcgcgccg gccagtttct gccgcaggag ctggagcttg ggctggatga cttccagtcg
     9001 ttttttggct ttgccgtcca ttgaggtcgt ccgtgcgatt ttacgtgcgg cggcgctgca
     9061 gattgcggag cacttcggcg gcttcgcccc gcaccaggcc gaagcaatcg agcaacagca
     9121 gcagcgcccg ctgcgggttg gcgctgtgca attcgccgcg gagctccatc accttgcgga
     9181 agaacgcggc ttcgtggcgg acgcgctggg cgaaggtcgc ggcgcaggcg acgccgcgcg
     9241 tttcttcgat ctcgaatagc gcggccagcg tgcggccggc cgcgagcaga tcgccggcct
     9301 gaatctgggc ggcgaacgcc tgcagggcgg ccgacgcgac gcgggaactc cctgtggtgc
     9361 gagtctgcgg cgcgggcagg gccggcgcgg cgacgtacag tgggccggtg agcgcgggta
     9421 tggtgttctc gatgacggcg tcggcgctgg gtgcgggctc ggtggcgacg gtgaacgtgt
     9481 cgccttcgcc cgggacggag ccgcgggaag tatccgcctc cggcggcagg ttcgccagca
     9541 tctggctgac gtgggccagc ttcaggccga cctgctcgta aaccgtgtcg atctcgttca
     9601 tgtacgagtc gccccgggcg tcggccagga tgtcctcggc ctgctccagg accttctcgg
     9661 tcaggcgcac cgcgagcacc gccttggcct cgcggagaac ctcgatcgct tgggcacgct
     9721 cgttcatggc tgtagttgaa ttgtttaacc cggagccgga ggcgcaggct cttcctcggg
     9781 gcgccgtcgc ctccggctcc gggttaaacg aggcctacct ccccgttcac tctatttggc
     9841 cgcggaggcg aagggaagcg tttgggcgat cctgcggtac gatcgcggca gccggagcag
     9901 gcggcgtcgc cggtgccttc agagtgcggc caacgaaccg caagccgccg ccgatcagga
     9961 ccatgagcac ggccagcgtg agattcacat tcatccagaa cttcgcgctg aactggaatc
    10021 gcttggcgag gtcggagcgt ccggccagga cgctggcaat gaaaaagatc ggcagggaca
    10081 agagaaactt gatgccgacg atcatctg
//
LOCUS       DASRJB010000126         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2859128, whole genome shotgun sequence.
ACCESSION   DASRJB010000126 DASRJB010000000
VERSION     DASRJB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2859128"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..785)
                     /locus_tag="VFB80_03695"
     CDS             complement(108..785)
                     /locus_tag="VFB80_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32890.1"
                     /translation="MGPEHSSQPAMEAAPLAPPAVPAELDQAVEAIVYDVHPALAQRD
                     YPYGRPVTNSARDLDQPLTQLLEAASGGVASVIESMSRKPDYVVPRRFGMSAILGIMT
                     ALAAIFGALHWLSAEPVFYAFFAIQSLTICIAQMLHGQTPRKASIIAGAVILPLFTLI
                     AANFSEQPVDGAFCLIIPFIPCGAVLGYLTGTCAAGIFLVMDKAEAYFKGESLDAPLH
                     APPSPVP"
     gene            1089..1688
                     /locus_tag="VFB80_03700"
     CDS             1089..1688
                     /locus_tag="VFB80_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HZN32891.1"
                     /translation="MSLAAPHYLLVAETTVSAGAPAVGGRWQFRLEQPGGQTALEAAD
                     VEPAAGPERLELLAIIRGLEALDQPSRVTLVSASRSVQAGLDFGLAAWRTNDWQWERF
                     GQLTAVKNADLWQRLDRLLEIHRVECRASRHKPADDLAAPPVRPQPQRRTRSGRLLRI
                     DPGPVAAAKIKEPKNQRTKRSRGSALSGFGSSVLRFLGF"
     gene            1752..>2233
                     /locus_tag="VFB80_03705"
     CDS             1752..>2233
                     /locus_tag="VFB80_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HZN32892.1"
                     /translation="MRSTVSLAGITALLDANLGRPTEVLGPQTIWREGRKMLAVRAFL
                     PGSRQAWVMDAATAAARPMRRLHPAGFYEAICPLDESPACESRPYPAYQLRVADHEGQ
                     MTTLHDPYAFPTTLTDFDLHLFGEGKLLRGYDKFGAQPRTIDGVKGVNFAVWAPNART
                     V"
BASE COUNT          418 a          718 c          731 g          366 t
ORIGIN      
        1 ggcgacgcga gccaccaccg tgccgccggc gtcaggccgc gcgacgttca ccacgcagtt
       61 gaaaaccggc ggcggatcga gccggctgac ggagtgaggc gcttcgctca tgggactggg
      121 ctcggcggag cgtgcagggg cgcatccagc gactcccctt tgaaataggc ctcggccttg
      181 tccatcacca ggaaaatgcc cgccgcgcac gtgccggtga gatagcccag cacggccccg
      241 cacggaataa acggaatgat caggcagaag gcgccatcga ccggctgctc gctgaagttg
      301 gcggcgatca gtgtaaacag cggcagaatc accgcgccgg cgatgatcga ggccttccgc
      361 ggcgtctggc cgtgcagcat ctgggcgatg cagatcgtca gcgactgaat cgcgaagaac
      421 gcgtaaaaaa ccggctccgc gctgagccag tgcagcgcgc cgaaaatggc ggccagcgcg
      481 gtcatgatgc ccaagatggc cgacatgccg aaccgccgcg gcaccacgta gtcgggcttg
      541 cggctcatcg actcgatgac gctcgcgaca ccgccgctcg ccgcttccaa caactgggtc
      601 agcggctggt ccagatcgcg ggcggaattc gtcacgggac gaccgtacgg ataatcccgc
      661 tgcgccagcg ccggatgcac gtcgtagacg atcgcctcga ccgcctgatc caattcggca
      721 ggcacggctg gcggcgcgag cggcgcggct tccatggccg gttgtgacga gtgttccggg
      781 cccatgccct gattctgact ggcggggagc cgcagggaaa gaaccaacca atcaaagaac
      841 cgaaacacca aggaaccaaa gaaccgaagc aagggtgtcc agatttggtt cttcggttct
      901 ttggatcttt ggttcttccg tctggcaagc tgtgccgtac agataagctg gagaggcgct
      961 attagcgcgg acgatagaag acacgtcggg tctcccaccg acagtctgcg gcgcaggatg
     1021 ctccgtttgg cggaacggat tcgcagtgga agttgacggc ccttgccccc aagtgcccac
     1081 ttgctgcgat gagtctggct gcacctcact atctcctggt cgccgagaca acggtctcgg
     1141 ccggcgctcc tgccgtgggc ggccgctggc aattcaggct cgagcagccc ggcggccaaa
     1201 ccgcgctcga agcggccgac gtcgagcccg ccgccggccc cgagcggttg gaactgctgg
     1261 ccattatccg cggcctggag gctctcgatc agccttcgcg ggtgacgctc gtctcggcca
     1321 gccgcagcgt gcaagccggg ctcgacttcg gtctggcggc ctggcggacg aacgactggc
     1381 agtgggagcg cttcggccag ctcaccgccg tcaaaaacgc cgacctgtgg cagcggctcg
     1441 atcggctgct cgagattcac cgcgtcgaat gccgcgctag ccgccacaag cccgccgacg
     1501 atctcgccgc gccgcccgtg cgcccacagc cgcagaggcg tacgcgcagc ggacgcttgc
     1561 tgaggattga ccccggaccg gtggcggcgg cgaaaatcaa agaaccaaag aaccaaagaa
     1621 ccaaaaggag tagagggagc gcactgtccg gttttggttc ttcggttctt cggttcttgg
     1681 gattttgatt ctggttcttg ggattttgat tcgcgcctcg atcgacaacc aggacttaag
     1741 gagaaggatt cgtgcggagc accgtatcgt tggcgggaat tacggcgttg ctggatgcga
     1801 acctgggccg ccccacggaa gtgctggggc cgcagacgat ttggcgggaa ggacgtaaaa
     1861 tgttggctgt gcgggcgttc ctccccggca gccggcaagc ctgggtcatg gacgccgcca
     1921 ccgccgccgc gcgccccatg cggcggctgc atcctgcggg tttttacgag gcgatctgcc
     1981 cgctggacga atcccccgcc tgcgaaagcc gcccgtaccc cgcctatcaa ctgcgagtcg
     2041 ccgaccacga ggggcagatg accaccctgc acgatccgta cgcctttccc acaacgctga
     2101 ccgattttga cctgcacctc ttcggcgaag gcaagctgct tcgcggctac gacaagttcg
     2161 gggctcagcc gcggacgatc gacggcgtga agggggtcaa cttcgccgtc tgggcgccga
     2221 acgcgcggac ggt
//
LOCUS       DASRJB010000127         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1729523, whole genome shotgun sequence.
ACCESSION   DASRJB010000127 DASRJB010000000
VERSION     DASRJB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729523"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFB80_03710"
     CDS             complement(<1..980)
                     /locus_tag="VFB80_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN32893.1"
                     /translation="MKRASLRSLLLSLLIASTAWAKPNVIVILADDLGNADLGVQGGK
                     DIPTPNIDSLAKSGVRCTQGYVSCPYCSPTRAGLNTGRYQTRFGHEFNEPGMAGRETF
                     GLPLDQKTIANRFKDLGYATGAIGKWHLGFSSDRRPTARGYDEFYGTLANTAFIKPAN
                     FVDSRKSSEVQVIDDPSFYTTDAYAERAEQFIAAHKDGPFFLYLPFNACHVPSEAPQK
                     YRDRFPGITEERRNLYAGMLSAMDDAVGRVLKAVRDAGQEENTLIVFLSDNGGPMTKM
                     GFNGSNNGVLRGQKGDTWDGGIHIPFFVQWKGQLPAGKVYEQPVISLDILP"
     gene            complement(1096..2535)
                     /locus_tag="VFB80_03715"
     CDS             complement(1096..2535)
                     /locus_tag="VFB80_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN32894.1"
                     /translation="MNAFRFLLVAALCTLGAAAQAAERPNFVFIYTDDQRWDALGVVQ
                     REQGERARFPWFSTPNLDKLAAEGVRFRNAFVVNSLCAPSRSVYLTGRYSHHNGVANN
                     HTPFPAENTATTWSNLLRQAGYATGYVGKFHHGQMPGKRPGFDYSASFIGQGRYTDCP
                     FEVNGVNTPTTGWVDDVSTDFAIDFIKKSKEKPFAVVVGFKAAHGPFDPPPRLTDKYA
                     GKEARPVPNQGLPPPYAGKFTAGKVKTATPEPKKKKAQAKAKANGRTMLQGYFGCLAA
                     VDENVGRILTTLDEQKLAPNTVVIFASDNGFYLGEHNLGDKRSAYDESLRIPLLVRAP
                     MLGEAARGKLVDRIALNLDLAPTLLDLAGAAIPGTMQGRSWRPLLTGDPARAEWRSAF
                     FYEYFFERNFNIPTVLAVRTDKAKLIKYPGHDDWTELFDLAADPYETKNLVNDPASKE
                     LLAAMQSEFDKQAKAVEFRIPPFADAVPD"
     gene            complement(2628..>2901)
                     /locus_tag="VFB80_03720"
     CDS             complement(2628..>2901)
                     /locus_tag="VFB80_03720"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN32895.1"
                     /translation="GMNVQAAGDAARVAHAGSATPTGLPADGVGTVTVERGVSEETID
                     HLRARGHRVGRARGGFGGYQGILIDWEHGILQGATETRKDGVAAGY"
BASE COUNT          472 a          917 c          978 g          534 t
ORIGIN      
        1 ggcaggatgt cgagcgagat gaccggctgc tcgtagacct tgcccgctgg cagttgcccc
       61 ttccactgga cgaagaatgg gatgtggatg ccgccgtccc aggtgtcgcc cttctgaccg
      121 cggaggacgc cgttgttgga gccgttaaag cccatcttgg tcatcggccc gccattgtcg
      181 ctaaggaaga cgatcagcgt gttctcttct tgcccggcat cacgcacggc tttcagcacg
      241 cggcccaccg cgtcgtccat ggccgagagc atgcccgcgt agaggttgcg gcgttcttcg
      301 gtgattcccg ggaaacggtc gcgatatttc tgcggggcct ccgacggcac gtgacaggcg
      361 ttgaacggca ggtagaggaa gaacgggccg tccttgtggg ccgcgatgaa ctgctcggcc
      421 cgctcggcgt aggcgtcggt cgtgtagaag ctcggatcgt cgatcacttg cacttcgctc
      481 gacttccgcg aatcgacgaa attcgccggc ttgatgaacg ccgtgttggc gagcgtgccg
      541 tagaactcgt cgtagccgcg agccgtcggc cgccgatcgg acgagaagcc caggtgccac
      601 ttgccgatcg cgccggtggc atagcccagg tccttgaagc ggttggcgat ggtcttctgg
      661 tcgagcggca ggccgaacgt ttcccggcca gccatgcccg gctcgttgaa ctcgtgtcca
      721 aaccgcgtct ggtagcggcc ggtgttcagg ccggcccgcg tcgggctgca ataggggcag
      781 gagacatagc cctgcgtgca gcggacgccg ctcttggcga gcgagtcgat gttcggcgtg
      841 ggaatgtcct tcccgccctg cacgccgaga tcggcgttgc ccaggtcgtc ggccaggatc
      901 acgatcacgt tgggcttggc ccaggcggtg ctggcaatca gcagactcag cagtagcgaa
      961 cggagagagg cgcgcttcat aacgttactc cgaggatggt tgcaactcgt gcccaagctc
     1021 tgcttcgagt gcctggaagc ggagcttcca gacgtgcgtt cccaagcgga gcttgggaac
     1081 gaggatcaac gagaactaat cgggtactgc gtccgcgaac ggtggaatgc gaaactcgac
     1141 ggctttggcc tgcttgtcga attcggactg catcgcggcg agcagctctt tcgacgcggg
     1201 atcgttcacc aggttcttcg tctcgtacgg atcggccgcg aggtcaaaca gctcggtcca
     1261 gtcgtcgtgg ccggggtact tgatcagctt ggccttgtcg gtgcgaacgg cgaggaccgt
     1321 cggaatattg aagttccgct cgaagaaata ctcgtagaag aacgcgctac gccactcggc
     1381 ccgcgcggga tcgcccgtaa gcagcggccg ccagctgcga ccctgcatcg tgccgggaat
     1441 cgccgcgccg gccaggtcga gcagcgtagg cgcgaggtcg aggttcagcg cgatgcgatc
     1501 aaccagcttg ccgcgggcgg cctcgcccag catcggtgca cgcaccagca gcgggatccg
     1561 cagcgactcg tcgtacgccg agcgcttgtc gcccaagttg tgctcaccca ggtaaaagcc
     1621 gttgtcgctg gcgaagatca cgaccgtatt cggcgctagc ttctgctcgt cgagcgtggt
     1681 gagaatccgc cccacgttct catcgacggc tgccaggcag ccgaagtaac cctgcagcat
     1741 cgtgcggcca ttggccttcg ccttggcctg ggccttcttc tttttcggct ctggcgttgc
     1801 cgtcttcacc ttgcccgccg tgaacttgcc ggcatagggc ggcggcaggc cctggttggg
     1861 aaccggccgg gcttccttcc ccgcgtattt gtcggtcagc cgcggcggcg gatcgaacgg
     1921 gccatgggcc gccttgaagc cgacgacgac ggcgaaaggc ttctctttgc tcttcttgat
     1981 aaagtcgatc gcgaagtcgg tcgagacgtc atcgacccag ccggttgtag gcgtgttcac
     2041 gccgttgacc tcgaacgggc agtcggtgta gcggccctgg ccgatgaagc tggccgagta
     2101 atcgaagccg ggccgcttgc cgggcatctg gccgtggtgg aacttgccga cgtagccggt
     2161 ggcatagccg gcttgccgca acaggttcga ccaggtggtc gccgtgttct cggccggaaa
     2221 gggcgtgtgg ttgttggcca cgccgttgtg gtggctgtag cggccggtga gataaacgct
     2281 gcgactggga gcacacagcg aattgacgac aaacgcgttg cggaaccgca cgccctcggc
     2341 cgcgagctta tcgagattcg gcgtgctgaa ccacggaaag cgggcccgct cgccctgctc
     2401 ccgctgcacg acgcccagcg cgtcccaccg ctggtcgtcg gtgtaaatga aaacgaagtt
     2461 cggccgctcg gccgcctggg cggcagctcc gagcgtgcac agggcggcca ctagcaagaa
     2521 tcggaaagca ttcatcggca gcgcgcggca ggagaatcgg ggcaaacgcc cgcgattgta
     2581 cgatgcgcgg gacgccggcg aaatgcgcgg cggctcaccc gcgacgatta ataccctgcg
     2641 gccacgccgt ctttgcgggt ctcggtcgcg ccttgcagga tgccgtgctc ccagtcgatg
     2701 aggatcccct gatagccgcc gaagccgccg cgggcccggc ccacgcggtg gccgcgggct
     2761 cgcaggtggt cgatcgtctc ctcggacacg ccccgctcga ccgttactgt gccgacgccg
     2821 tccgcgggca ggcccgtcgg cgtcgcgctg ccggcgtgtg ccacgcgggc cgcgtcgccg
     2881 gccgcctgta cgttcatccc a
//
LOCUS       DASRJB010000128         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2293877, whole genome shotgun sequence.
ACCESSION   DASRJB010000128 DASRJB010000000
VERSION     DASRJB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293877"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..148
                     /locus_tag="VFB80_03725"
     CDS             11..148
                     /locus_tag="VFB80_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32896.1"
                     /translation="MKNFYAEKVLLEQPFVKENSLSVGKYAESKGMQVKKFVHWELGK
                     G"
     gene            227..970
                     /gene="pyrH"
                     /locus_tag="VFB80_03730"
     CDS             227..970
                     /gene="pyrH"
                     /locus_tag="VFB80_03730"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZN32897.1"
                     /translation="MSSTPVLPYKRVVLKLSGESFSHGGERGLGMQEVVQIASQVKQA
                     ADAGCQIGLVIGGGNILRGSHFKNANAGIQEATAHYMGMLATVINALALQDALESLGC
                     ETRVMTAIHMAGVAEPYIRRRAKRHLEKGRIVILAAGTGSPFVTTDTAAAQRAMELDA
                     EILLKATRVDGVYSDDPERNPHAMLYSELDYATVRERNLRVMDSTAIAHCMEHNMPIL
                     VFNFRKDGNILRAVRGEKVGTRISSKPTA"
     gene            1068..2192
                     /gene="glf"
                     /locus_tag="VFB80_03735"
     CDS             1068..2192
                     /gene="glf"
                     /locus_tag="VFB80_03735"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HZN32898.1"
                     /translation="MAFDYVIVGAGLFGSVLARRVAEAGRTVLLVERRPHIGGNCYSE
                     SIGGIEVHRYGAHIFHTDSVRVWEFVNRFTRMNHYRHRGVVRHGERLFSFPINLLTLH
                     QLWGVLTPAEAEARLARERLPIDRPQNLEEWILSQVGPELYETFIRGYTAKQWGRDPA
                     ELPAAIIRRLPIRLTWDDSYFSDRYQGIPEQGYTRMFENLLDHRGIRVQTGVDFLTAR
                     NELLGAGRRLVYSGQIDQFFDYRYGPLEYRSLRFAIEHRSGDFQGAAVVNYAAADVPY
                     TRIIEHKHFALETSDTTVLTCEYPQPFAPGGEAYYPIRDAKNSELFDRYARLAAEEAP
                     QVLFGGRLGSYQYYDMHQVIGAALAAADTELGLTAARRAA"
     gene            2199..2975
                     /locus_tag="VFB80_03740"
     CDS             2199..2975
                     /locus_tag="VFB80_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32899.1"
                     /translation="MTHRNLVISPVGDDSQHATWLAAAAEREFDLALVYFGQQPGRFR
                     ADADCYLAERGFKFHLIERMLGELGPRLDEYEFIWLPDDDIASTTHDINRLFAVAREY
                     LLAIAQPAIASGDVSYRVVRQQAGLLLRYTQFVEVMCPLLSRAALDRVRPTLTENKSA
                     WGVDWAWTRLIPPHKMAIIDAVGVHHTRPLHSGAGYQRMRDHGVDPHRDLLEMAARYG
                     VTRRQRRQAKHASRRVRAVTLDGRPTWAGRPWWQFWRRAA"
     gene            complement(2991..3938)
                     /locus_tag="VFB80_03745"
     CDS             complement(2991..3938)
                     /locus_tag="VFB80_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32900.1"
                     /translation="MRVVSVTPAGRRRYLAALVPHLLRQRHVIAEHHWWLNTSDEADV
                     RYIREITARHPDFFKICDLPGDPQLNIGQSIWRFFRHYVQPGTLYIRFDDDIVYMADD
                     AVVNLVRYRLTHREPLLVVGNIVNNAVCAHFQQRAGLIPLAWGKVQNECLDRNAWRRG
                     PFPRKLHQLFLNDLLQGREDRWKQIPMRIDGRRRFSINVISWMGDDLRQVPELASDRI
                     EEEPFLTETLPARMNRPNAACPSALFAHFAYYSQRPYLEWTWPELIGHYQRIASQLSP
                     EPLRELPLTLLRHAAWRMSRPVGRLREKLLMRWRGQQAA"
BASE COUNT          862 a         1493 c         1509 g          823 t
ORIGIN      
        1 cgaaggccgg ctgaagaact tctacgccga gaaggtcctg ctcgagcagc cgttcgtgaa
       61 ggagaacagc ctctccgtcg gcaagtacgc cgagagcaag ggcatgcagg tcaagaagtt
      121 cgtgcactgg gagctgggca agggctagga atttcagatt tcagatttgc gatttgaaat
      181 ttgaaatctg aaatctgaaa tcccaaatct gaaattgcgt accgaaatgt cttccactcc
      241 tgtgttgccc tataagcgcg tcgtcctgaa gctgtccggc gagagcttca gtcatggcgg
      301 cgagcgcggc ctcggaatgc aggaggtcgt gcagatcgcc agccaggtca agcaggccgc
      361 cgacgccggc tgccagatcg gcctggtgat cggcggcggc aacattctcc gcggctcgca
      421 tttcaagaat gccaacgccg gcatccagga agccaccgcc cattacatgg ggatgctggc
      481 gaccgtcatc aacgccctgg ccttgcagga cgcactggag tcgctcggct gcgagactcg
      541 cgtgatgacc gcgatccaca tggccggcgt ggctgagccc tacattcgcc ggcgggccaa
      601 gcggcacctg gaaaaaggcc gcatcgtgat cctcgccgcc gggaccggca gcccatttgt
      661 gacgacggac accgcggccg cccagcgggc gatggagctc gacgccgaaa tcctgctcaa
      721 ggcgacccgg gtcgatggcg tctacagcga cgacccggag cgcaacccgc acgcgatgct
      781 ctacagcgag ctcgactatg ccaccgtccg cgagcgaaac ctccgcgtga tggacagcac
      841 cgcgatcgcc cactgcatgg agcacaacat gccgatcctg gtcttcaact tccgcaagga
      901 cggcaacatc ctccgcgccg tccgcggcga gaaggtcggc acccgcatca gcagcaagcc
      961 gacagcttga tactgcttgc gttgcctgcg cggttgctct ttgaaaccga tgccgacgcg
     1021 gctatcttcc gcccgcatgg gggagcgaac tccagcggtg gatcgccgtg gcctttgact
     1081 acgtcatcgt cggcgcgggt ctgtttggca gcgtcctggc gcggcgcgtg gccgaggcgg
     1141 gccgcaccgt gctcctcgtc gagcgccggc cgcacatcgg cggcaattgc tactccgaat
     1201 cgatcggcgg catcgaggtc caccgctacg gcgcgcacat cttccacacc gacagcgtcc
     1261 gcgtctggga gttcgtcaac cgctttacgc ggatgaacca ctatcgccat cgcggcgtgg
     1321 tgcggcacgg cgagcgactg ttctcgttcc cgatcaacct gctcacgctg catcagctct
     1381 ggggcgttct cactccggca gaggccgaag ccaggctggc tcgggagcgc ctgccgatcg
     1441 acaggccgca aaacctggaa gagtggatcc tgtcacaagt cggccccgag ctgtacgaaa
     1501 ccttcatccg cggctacacc gccaagcaat ggggccgcga cccggccgag ttgcccgcgg
     1561 cgatcatccg ccggctgccg atccgcctca cctgggacga cagctatttc agcgaccgct
     1621 accagggcat tcccgagcag ggctacacgc gaatgttcga gaacctgctc gaccaccgcg
     1681 gaatccgggt ccaaacgggc gtcgatttcc tgacggcgag gaacgagctg ctcggtgccg
     1741 gccggcggct ggtctattcc gggcagatcg accagttctt cgactatcgc tacggcccgc
     1801 tcgagtatcg ctcgctgcgc ttcgcgatcg agcaccgcag cggggatttc caaggcgcgg
     1861 cggtggtcaa ttacgcggca gcggacgtgc cctacacccg catcatcgag cacaagcact
     1921 ttgcgctaga aacctcggat acgacggtgc tgacctgcga gtatccgcag cccttcgcgc
     1981 ccggcggcga ggcctattat ccgatccgcg acgccaaaaa ctcggagctc tttgaccgct
     2041 atgctcgcct ggcggcggaa gaagcgccgc aggtcctgtt tggcggccgg ctgggcagct
     2101 accagtacta cgacatgcac caggtgatcg gcgcggcgct ggcggctgcg gacaccgagc
     2161 tcggcctgac ggcggcgcgg cgcgccgctt gacgaaccat gacgcatcgc aacctggtca
     2221 tttcgcccgt cggcgacgat tcgcagcacg cgacgtggct tgccgccgcg gccgagcggg
     2281 agtttgacct ggcgctcgtt tactttggcc agcagccggg gcggtttcgg gcggatgccg
     2341 attgctatct ggccgagcgg ggcttcaagt tccatttgat tgagcgcatg ctcggcgagc
     2401 ttggaccgcg acttgacgaa tatgaattca tctggctgcc ggacgacgac atcgcgagca
     2461 cgacgcacga catcaaccgg ctgttcgcgg tggcccgcga atatctcctg gccatcgccc
     2521 agccggcgat cgccagtggc gatgtttcgt atcgagtcgt gcggcagcag gccggcctgc
     2581 tgttacgcta cacgcagttc gtcgaggtga tgtgcccgct gctgtcccgc gcggcgctcg
     2641 atcgcgtgcg gccgacgctg accgagaaca agtctgcctg gggcgtcgat tgggcgtgga
     2701 cgcgcctgat tcctccgcac aaaatggcga tcatcgatgc ggtcggcgtc caccacaccc
     2761 ggccgctgca ctcgggcgcg ggctatcagc ggatgcgcga ccacggcgtc gatccgcacc
     2821 gcgacctgct ggagatggcc gcacggtacg gcgtgacccg gcgacagcgg cggcaagcca
     2881 agcacgccag ccgccgcgta agggccgtaa cgctcgatgg gcgtccaacc tgggccggcc
     2941 gcccgtggtg gcaattctgg cggcgggcgg catgagtcac aggcgtgcgc tcaggccgcc
     3001 tgctggccgc gccagcgcat taagagcttt tcccgcagcc ggccgaccgg gcggctcatt
     3061 cgccacgcgg catgtcgcag cagcgtcagc ggcaattcgc gcagcggttc gggcgacaac
     3121 tgcgaggcaa tgcgctgata gtgcccgatc agctcgggcc aggtccattc gagatagggc
     3181 cgctgcgagt agtaggcgaa atgggcgaag agcgcgctag ggcacgcggc gttgggccgg
     3241 ttcatgcgtg ccggcagcgt ctcggtcaga aagggctcct cctcgatccg gtcgctcgcc
     3301 aattcgggca cttgtcgcag gtcgtcgccc atccaggaaa tcacgttgat tgaaaatcgc
     3361 cgccggccgt cgatccgcat tggaatctgc ttccaccgat cttcgcgacc ctgaagcaag
     3421 tcgttcagga acagctgatg cagcttccgc gggaacggtc cgcgccgcca cgcgttgcgg
     3481 tccaggcact cgttctggac ctttccccag gccagcggaa tcagtccggc ccgctgctga
     3541 aaatgggcgc agaccgcgtt gttgacgatg ttccccacga cgagcagcgg ctcgcgatgc
     3601 gtcagccggt agcggacgag gttcacgacg gcgtcgtcgg ccatgtagac gatgtcgtcg
     3661 tcaaagcgga tatagagcgt gccgggctgg acatagtggc gaaagaaccg ccagatgctc
     3721 tggccgatat tcagctgcgg atcgcccggc aagtcgcaga tcttgaaaaa gtcgggatgc
     3781 cgcgcggtga tctcgcggat gtaccgcacg tcggcctcgt ccgacgtgtt cagccaccag
     3841 tgatgttcgg caatgacgtg ccgctgccga agcaggtgcg gcaccagggc agccagatat
     3901 cgccgcctgc cggcgggcgt gacgctgacg acgcgcatgg ctctctcccg aacgtggtcg
     3961 ggcggagtct aagggcgggc acctccacgg cgaagcccaa ctgcgagggc gaatcttgtt
     4021 ttgcctgaat cttgtgcagc agccgttcgg cgacgggcat tttcgggtgg ctcggcatgg
     4081 tgtgtacgcg aagcgtcaaa gtttgttgat gtcctaacgc attggccgca aaagagttct
     4141 gaacgttgct aatcactaga gccagcacac cggccccttt ggcacggagt ctgcaaatag
     4201 gggtctcacc gccggggacg agaaaaaacg agacggcaac gaaagcttgt tcagacgtta
     4261 acgctcgccc ggtctaagga gaatcgttcg ctccgcatga ttggcgccgc agcttgccgg
     4321 ccagttcctg ctggccagtg tctcctgcgg ctgaaccagg attgcagacg agaaacgccc
     4381 gttgctgccc ggcgcttgaa tctcagggaa gggagaggtc cttgggattc gggtgtccgg
     4441 gcgcaccggg gccggaattg aagcggcgcc gccgccgacg atatacaggc cgccccaccc
     4501 cgcccgaagc ccgcgggtcg attttcgagt cgatccgcgg gctttctcat ttcttggaaa
     4561 agacgtagcg tctggggaat aatgaaagcg cagtgcgcat ctatacaagc ggatcataat
     4621 ccgcgccgcg caacgcgatt tgtgaacatt ggtacgctat ttgcatgaca gttccatttg
     4681 gcgcgaa
//
LOCUS       DASRJB010000129         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_600724, whole genome shotgun sequence.
ACCESSION   DASRJB010000129 DASRJB010000000
VERSION     DASRJB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600724"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFB80_03750"
     CDS             <1..279
                     /locus_tag="VFB80_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN32901.1"
                     /translation="QGELPIADYIARWRERIVNVHIEDMRAGVHEHLMFGEGQMHFPP
                     IIEALDRTGYKGGLHVELSRHSHEAPTAAKKAFDFLQPLIESQLTTDH"
     gene            295..1617
                     /locus_tag="VFB80_03755"
     CDS             295..1617
                     /locus_tag="VFB80_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HZN32902.1"
                     /translation="MSDHVILLSIPGLRSQDLAAMPNLSRLTAGGDRATLAPSFPAVT
                     WPVQANMLTGKLPRDHGVVANGFYWRDKHEVEMWTAWNEKIQQPQIWDLLHKHDPGLK
                     SAVWFPMLSKGCGADYVCMPAPIHNPDGSESLWCYTKPTELYGTLRDTFGHFPLMNFW
                     GPMAGIKSTAWIAETAAWAMQQYRPSFFYIYLPQLDYAAQKTGPDSPAAQKAVQELDE
                     VLGKLIAAGSAAYQGQPLWLIASEYVITPVDHVTYPNRVLREAGLLQVKNDGAGELID
                     FANTPTWALVDHQFSHVFVKNRDAATIARVAALFQGRAGIADVLSGAGRAKYTLDHER
                     SGDVILISTPNSWQAYYYWQDDKNAPKFARTVDIHNKPGYDPVELHFDFATKSIPLDA
                     TLIKGSHGAPATTDAQRGVLLSSQKGTFDTRPHADADICELVLRQFGI"
     gene            1627..2901
                     /locus_tag="VFB80_03760"
     CDS             1627..2901
                     /locus_tag="VFB80_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HZN32903.1"
                     /translation="MAMEPITLFSRNVDPAGVARRLRELAKSVQIDGPDDRWQNAVVT
                     FKERGKAKTLTLTHDPDYYSEPNWSEQMNGMRGYFSRFPESDRKQPVLTLTTSLKFSL
                     GTLFEPDFDAAGDPRLDVVFSIAEFLDGVLFTPSSLRDAQGRILLSAGGEDEEDPEAV
                     WPRVMGEVDVADPMGAQMHEISRPKQAGEDDDEPEAPTAQRVARRALVLTAVTARAIL
                     EQDAGDPQAKATYKRLLDWIKQLSIDDEFEPDEWRVVQRPLGKLEQQDQINATWRLEG
                     LVVLAWALNRYEIPPHDELVRLDPLWESLGFMDDAASRRLLENPTLRSRDELRVQRNQ
                     LLALHWRLRNFYLNQEILDFAEFARTCWFGPLDVTGLSLVDSDLALQGARLDRAAPED
                     FSTAHSAALERHRAANWLWEGPACYSEASEAT"
BASE COUNT          588 a         1054 c          963 g          475 t
ORIGIN      
        1 cagggcgagc tgccgatcgc cgactacatc gcccgctggc gcgagcggat cgtcaacgtg
       61 cacatcgaag acatgcgggc cggcgtccac gagcacctga tgttcggcga aggccagatg
      121 cactttccgc ccatcatcga agccctcgac cgaaccggct acaaaggagg tctgcacgtc
      181 gagctctcgc gccacagcca cgaagcgccg accgccgcca agaaggcgtt cgactttctg
      241 cagccgctga tcgaaagcca actgaccact gaccactgac aactgaccac ggacatgtcc
      301 gaccacgtca tcctcctttc catccccggc ctccgctcgc aagacctggc cgcaatgccg
      361 aacttgtcgc ggctgacggc gggcggcgat cgggcgaccc tggcgcccag ctttcctgcg
      421 gtcacgtggc cggtgcaggc caacatgctc acgggcaagc tgccccgcga ccacggggtc
      481 gtcgccaacg gcttctactg gcgcgacaag cacgaagtcg aaatgtggac cgcctggaac
      541 gagaagatcc aacagccgca gatttgggat ctgctgcaca agcacgatcc tgggctgaag
      601 tcggcggtgt ggttcccgat gctgagcaag ggctgcggcg cggactatgt ctgcatgccc
      661 gcgccgatcc acaaccccga cggcagcgaa tcgctgtggt gctacacgaa gccgaccgag
      721 ctgtacggca cgctgcgcga cacgttcggg cattttccgc tgatgaactt ttgggggccg
      781 atggcgggaa tcaaatcgac cgcctggatc gccgagaccg ctgcctgggc gatgcagcaa
      841 taccggccga gcttcttcta catctatctg ccgcagctcg actacgccgc ccaaaagacc
      901 ggccccgaca gccccgccgc acagaaggcc gtgcaggagc tggacgaagt gctgggcaag
      961 ctgatcgcgg ccggatccgc agcgtaccag ggccagccgc tctggctgat cgccagcgaa
     1021 tatgtcatca cgccggtcga tcacgtcacc tatcccaacc gcgtcttgcg cgaggccggc
     1081 ctgctccaag tgaaaaacga cggggcaggg gagctgatcg acttcgccaa tacccccacc
     1141 tgggccctgg tcgatcacca attttcgcac gtgttcgtca agaatcgcga cgccgccacg
     1201 attgcgaggg tcgccgcgct gttccagggc cgcgccggca tcgccgacgt cctgagcggc
     1261 gcgggccgcg cgaaatacac cctcgaccac gagcgctccg gcgacgtaat cctcatctcc
     1321 acgccgaaca gctggcaggc ctattactac tggcaagacg acaagaacgc ccccaagttt
     1381 gcccgcaccg tcgatatcca caacaagccc ggctacgacc cggtcgaatt gcacttcgac
     1441 ttcgccacca agagcatccc gctcgacgcc acgctgatca aaggctccca cggcgctccc
     1501 gctacgacgg acgcccagcg cggcgtcctg ctcagctcgc aaaaagggac gttcgacacc
     1561 cggccgcatg ccgatgccga catctgcgag ctggtgctgc ggcaatttgg aatctgaggg
     1621 tgtgccatgg cgatggagcc catcacgctg ttctcccgga atgttgatcc ggccggcgtc
     1681 gctcgccggc tgcgggagct ggccaagagc gttcagatcg acgggccgga cgaccgttgg
     1741 caaaacgcgg tcgtcacgtt caaggagcga gggaaagcga aaacgctgac ccttacgcac
     1801 gacccggact actactcgga gcccaactgg tccgagcaaa tgaatgggat gcgcgggtat
     1861 ttctcacgct tccccgagag cgatcgcaag cagccggtgc tgacgctgac cacgagcttg
     1921 aagttctcgc tgggcacgct gttcgagccg gacttcgacg ccgccggcga tccaaggctg
     1981 gatgtagtct tctcgatcgc ggaattcctg gacggcgtgc tgttcacgcc ttcctcgctg
     2041 cgcgacgccc agggccgcat cctgctcagc gccggcggcg aggacgagga agaccccgag
     2101 gcggtctggc cgcgggtcat gggagaagtg gacgtcgccg acccgatggg tgcccagatg
     2161 cacgaaatct cccggcccaa gcaggcgggc gaggacgatg acgagcccga agcgccgacc
     2221 gcgcaacgcg tcgcccggcg ggcgctggtc ctgactgccg tgactgcgcg ggccattctg
     2281 gagcaggacg ctggcgatcc ccaagcaaag gcgacgtaca agcgcctcct ggattggatc
     2341 aagcagctct ccatcgacga cgagttcgag cccgacgaat ggcgcgtcgt gcagcgcccg
     2401 ctcggcaagc tcgagcagca ggaccagatc aacgccacct ggcggctgga agggctggtc
     2461 gtgctggcct gggccctgaa tcgctacgag attccgccgc acgacgagct ggtgcggctc
     2521 gatccgctct gggaaagcct cggcttcatg gacgacgcag ccagtcgccg gctgctggaa
     2581 aacccgacgc tgaggtcgcg cgacgagctg cgcgtgcagc gcaaccagtt gctggccctc
     2641 cattggcggc tgcggaactt ctatctcaac caggagatcc tcgatttcgc cgagttcgcc
     2701 cgcacctgct ggttcggccc gctcgacgtg acggggctgt cgctcgtcga tagcgacctt
     2761 gcccttcaag gcgcgcggct cgaccgcgct gcgcccgaag acttctccac cgcccacagc
     2821 gccgccctag agcggcaccg cgccgccaac tggctctggg aaggccccgc ttgctattcg
     2881 gaagccagcg aggcgacgtg aagccgtgga cgcgactctg caattccttt ggaaccgccg
     2941 ctccgcgggc cgcccggccg atgaagcgtc caaatgggcg acgacgttgc tcgagcaggg
     3001 ccgcgagtca gaggcgatcc ggcgcttggc cggcaacaca aatctggagc gcgagacggc
     3061 cgatcagctc gtgaagcagg
//
LOCUS       DASRJB010000130         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2012368, whole genome shotgun sequence.
ACCESSION   DASRJB010000130 DASRJB010000000
VERSION     DASRJB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012368"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..2224
                     /locus_tag="VFB80_03765"
     CDS             290..2224
                     /locus_tag="VFB80_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZN32904.1"
                     /translation="MCAQPRVLSFILAALGLVSLGSAAPAAKPTPAEALKLVPVQREI
                     DYDVPAAKEIEICIVDVETVGGITGWVVKTPGGQILRRFLDTNGDNRVDQWCYFKDGI
                     EIYRDIDVNFNNKADQYRWLGIAGTRWGLDDDENGRIDSWKVISPEEVTAEVVAAFRD
                     RDTARFQRLLLTADDLKSLGLNQKHVTDLTEKVATATRTFAGVADKQRVVTPQSEWIH
                     FGASKPGVIPAGTDGSTKDIVVYDNVTAVVETAGAAAAGAAKQHGQLIVGTLVKVGDA
                     WKLFDLPKNLTGNQTAAAGGYFFLLGAGARAETDVPSAAGGVSPEMQKLTGDLEQLDK
                     RLASATTPAEQARLNATRADLLDKIIAAAPADQRGLWVRQYTETVSAAVQSGAYPDGV
                     GRLQSLLTKVTNLPDGAELIPYVKQRLLTAEYNRDIAVKDADFEKVNTRYIEQLEQFV
                     KEYGTSPDAAEAMLQLAIDAEFTKQREKAIAWFGRIATDFPKSELAEKAAGAKFRLVS
                     VGKSLGLKGKTLDGRNFDTATANGKLVLIHYWTTTCGPCKDDMELIKSLLAKYGGQGF
                     YPVGINLDNDVKDASNFLKSKPLSWPQLYETGGLDSRLATQYGILSLPTMILIGKDGR
                     VIDANIQSGELEAEVKKQLR"
     gene            2285..>2853
                     /locus_tag="VFB80_03770"
     CDS             2285..>2853
                     /locus_tag="VFB80_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN32905.1"
                     /translation="MSLPTLADIEYPESDGQPMGETDLHRLWMIRLYELLRWRYADQQ
                     VYIGSDLLVYYEEGKPQRFCVPDVFVVLDCPSGHRRVFKTWAEGRVPDVVIEVTSLSS
                     RREDEVFKPKTYSRIGVKEYFLFDPTNDYLSPALIGFRLSGDETQRILPDASGSLHCA
                     TLGVRLWLEGDELVLADAATGQRLLTEAEA"
BASE COUNT          557 a          885 c          942 g          469 t
ORIGIN      
        1 gctagatccg tggcaatccg tgcaaatccg cggctcattc ccggtcgccc gcgacggact
       61 accgcctact gacggcagag cacgcataat gcgcataatc ggcaacccgc attttctgcc
      121 gcaaagcgtt ggtggtacag cacttgcaac gcaaactgac tggcggagaa atactttgga
      181 tacgcgcgca tggccgacgg gtgccgacga gttttggtgt cccctcgtgg cggggctgcc
      241 gataaaatca gcgccggtta agttgcccat tttgaaggaa acggaagcca tgtgtgctca
      301 accccgcgtc ttgtcgttca tcctggccgc cctggggctg gtctcgctcg gatcggccgc
      361 gccggccgcc aagccgacgc cggccgaggc cttgaagctc gtcccggtcc agcgggaaat
      421 cgattacgac gtgccggccg ccaaggagat cgagatttgc atagtggacg tcgagacggt
      481 cggcggcatc acgggctggg tcgtaaaaac gccgggcggc cagattctgc ggcggttcct
      541 cgataccaac ggcgacaacc gcgtcgatca gtggtgctat ttcaaggacg gcatcgagat
      601 ctaccgcgac atcgacgtca acttcaacaa caaggccgat caatatcgct ggctgggaat
      661 cgccggcacg cggtggggcc tggatgacga cgagaacggc cggatcgatt cgtggaaggt
      721 catctcgccc gaagaggtga cggcggaagt cgtcgccgcc ttccgcgacc gcgacaccgc
      781 ccgctttcag cggctgctgc taaccgccga tgacctgaag tcgctcggcc tcaaccagaa
      841 acacgtcacc gatctgaccg agaaggtggc cacggccact cgcacgttcg ctggcgtcgc
      901 cgacaagcag cgggtcgtga cgccgcaatc ggagtggatt cacttcggcg cgagcaagcc
      961 gggcgtcatt cccgccggca ccgacggctc gaccaaggac atcgtcgtct acgacaacgt
     1021 gaccgccgtc gtcgagacgg ccggcgctgc cgcggctggc gcagccaagc agcacgggca
     1081 gctcatcgtc ggcacgctgg tcaaagtggg cgacgcctgg aagctgttcg acctgcccaa
     1141 gaacttgacg ggcaatcaga cggcggccgc aggcggctac ttcttcctgc tcggcgccgg
     1201 tgcgcgggcc gagacggatg tcccctcagc cgccggcggc gtcagccccg agatgcaaaa
     1261 gctgaccggc gacctggagc agctcgacaa gcggctggct tcggccacca cgccggcgga
     1321 gcaggcccgc ttgaacgcga cccgggccga tctgctcgac aagatcatcg ccgccgcgcc
     1381 ggccgatcag cgcgggctgt gggtgcgaca atataccgag acggtttcgg ctgccgtgca
     1441 gtcgggggcg tatcccgacg gcgtcggccg cttgcagtcg ctgctgacca aggtgacgaa
     1501 cctgcccgac ggcgcggagc tgattcccta cgtcaagcag cggctgctca cggctgaata
     1561 caaccgcgac atcgcggtaa aggacgccga ctttgagaag gtcaacacgc ggtacatcga
     1621 gcagctcgag cagttcgtga aggaatatgg caccagcccc gacgcggccg aggcaatgct
     1681 ccagctcgcg atcgacgccg agttcaccaa gcagcgcgag aaggcgatcg cctggttcgg
     1741 gcggatcgcc acggattttc ccaagtcgga gctggccgaa aaagcagccg gcgcgaagtt
     1801 ccggctggtc tcggtgggca aatcgctcgg cctgaaaggc aagacgctcg acgggcggaa
     1861 cttcgacacg gccaccgcca atggcaagct ggtgctgatc cactactgga cgacgacctg
     1921 cgggccctgc aaagacgaca tggagctgat caagtcgctg ctggccaagt acggcggcca
     1981 gggcttttat ccggtcggca tcaacctcga caacgatgtg aaggacgcga gcaatttcct
     2041 gaagtccaag ccgctctcgt ggccccagct gtacgaaacc ggcggcctcg acagccggct
     2101 ggccacgcag tacggcatcc tctcgctgcc gacgatgatc ctgatcggca aggacggccg
     2161 cgtgatcgac gccaacatcc agtccggcga gctggaggcc gaggtgaaaa aacaattgcg
     2221 ataggacggc ggcggatata atgacgatgc gcggaaggaa gtgggtccgc gcgggattcg
     2281 tgccatgagc ctgccaacgc ttgccgacat cgagtatccg gaatcggacg ggcagccgat
     2341 gggcgagacc gacctgcatc ggctgtggat gatccggctg tacgagctgc tgcgctggcg
     2401 gtacgccgac cagcaggtct atatcggcag cgatttgctc gtgtattacg aagagggcaa
     2461 gccgcagcgg ttctgcgtgc cggacgtgtt cgtcgtgctc gattgtccgt cgggccatcg
     2521 gcgggtcttc aagacctggg cagaaggtcg cgtgccggac gtagtcattg aagtgacctc
     2581 gctgagctcg cgccgcgagg acgaggtgtt caagccgaag acgtacagcc gcatcggcgt
     2641 gaaggagtat ttcctgttcg acccgacgaa cgactacctt tcgcccgcgc tcattggatt
     2701 ccggctatcg ggcgacgaga cgcagcggat cttgccggat gcctcgggtt cgctgcactg
     2761 tgcgacgttg ggggtgcggc tgtggctgga gggtgacgag ttggtgcttg ccgacgcggc
     2821 caccggtcag cgactactga cggaagccga agc
//
LOCUS       DASRJB010000131         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_320759, whole genome shotgun sequence.
ACCESSION   DASRJB010000131 DASRJB010000000
VERSION     DASRJB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320759"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1868)
                     /gene="amt"
                     /locus_tag="VFB80_03775"
     CDS             complement(237..1868)
                     /gene="amt"
                     /locus_tag="VFB80_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN32906.1"
                     /translation="MSRNRIWIACGLAALAALTALLALGSSLTAQDAPKTEPAAAAPA
                     ETPKVAEPAAPAPAAEPAKEEAKETAAPAPVGPTNVDLKVMADTLWVMVTAMLVFFMN
                     LGFATVESGMCRSKNCVNILSKNFIVFAVTSIGFLLLGWGIMFGNGNGFIGTSGLWMV
                     GGADNSPATGSAYAGDYSAISWTGVPLWAKFFFQLVFAGTAATIVSGAVAERIKYHTF
                     IVFSFIMAVAIYPVVGHWIWGGGWLAAKWNFFDFAGSTQVHSIGGWAALTGAAILGPR
                     LGKYGKDGKINAIPGHSLMAATIGCFVLWFGWFGFNPGSTMAVDVNAMAEIAVTTNTA
                     AAAAALSATVACWFLLGKPDLGMTLNGCLAGLVAITASCAFVTVGASLIIGLIAGVLV
                     VLSVIAFDKIKIDDPVGATSVHLVNGVFGTLCVGLFAEPSRVAARCLAPANAWKGLFY
                     GGGFDQVIGQLVGIGATAVYVLLLSSAAWLVLKYTMGLRVSAEEELEGLDIGEHGHYA
                     YPPQLVVDGYGSGAPVPAPAGTAFPAHAKPSTESV"
     gene            complement(2265..>3490)
                     /locus_tag="VFB80_03780"
     CDS             complement(2265..>3490)
                     /locus_tag="VFB80_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN32907.1"
                     /translation="CDSCCGCDFLSLWPCGCPLECLGEACKLWEPCCEDSPWTGAGWL
                     AAGFVWNPYSPVDRFNGPMTWNDRANEGQLNELFYYFGRTANTEGCGWDWGARVDALG
                     GTSYRWNTSAGFESSWGINGKFYGIAIPQLYGELAYNDLSVKVGRFYSPVGYYVIGTA
                     NNYFPVLPYTFQYGEPFTHTGVLATYKAGDDLVLGGALTHGWDSSDNTGNPHAGGLAM
                     ATLTIDEQRSLAYVGVFGNEPNFSGVNLRSNGFGYTARYLQTLVYINKISDDVTAVLQ
                     SDFGRQHDAVVPGEVASWYGVNGYLYWNQTCRLQWGLNGEWFRDDGGFRVGQVLPSFG
                     SPNARGYARSGFDGSFYRLMFGPKYFFTPNIYGRAAFAADWYAGDRNAINEVPFDDGT
                     KNHQQVIVFDLIATF"
BASE COUNT          667 a         1101 c         1142 g          580 t
ORIGIN      
        1 cttgtggggc tggatgatgg cgatgatgag cttcataggt cgagttcaga gttcaaagtt
       61 caaggttcaa agttagtgga ccatcggcgt ggttgcaact ttgaactccg aactcgcaac
      121 tcgaaactca aagaggaata ccccggcctg ggcgagtgag gcggacactc gcccaggcca
      181 ttggggtatc cttcaggccc aaaagagccg aagtgctttc gcgtggaagc tgagcctcaa
      241 actgactccg tgctcggttt ggcgtgagcc gggaaggcgg tcccggccgg ggcgggcacc
      301 ggagcgccgc tgccatagcc atcgacgacg agttgcggcg ggtaggcgta gtggccgtgc
      361 tcgccgatgt ccaggccctc gagttcttcc tccgcggaga cccgcaggcc catcgtgtac
      421 ttcaggacca gccaggcggc cgacgacagc aggagcacgt acaccgcggt cgcgccgatg
      481 ccgaccagtt gcccaatgac ctgatcgaag ccgccgccat aaaacaaacc cttccaggca
      541 ttggccggcg ccaggcaacg ggcggcaacc cggctcggct cggcaaacag gccgacgcag
      601 agcgtgccga acacgccgtt gaccaggtgc accgatgtag cacccacggg gtcgtcgatc
      661 ttgatcttgt cgaaggcgat gaccgacagc acgacgagca cgcccgcgat gaggccgata
      721 atcagcgacg caccgacggt gacaaacgcg cacgacgcgg tgatcgccac gaggccggcc
      781 aggcagccat tcagggtcat tcccaggtcg ggcttgccaa gcaggaacca gcaagccacc
      841 gtcgcgctga gggcggcggc ggctgccgcg gtatttgtcg ttacggcgat ttcggccatc
      901 gcgttgacat cgacggccat cgtgctgccg gggttgaagc cgaaccagcc gaaccagagg
      961 acgaagcagc cgatcgtggc ggccatcaag ctgtggccgg gaatcgcgtt gatcttgccg
     1021 tccttgccgt acttgccgag gcgcggcccg agaatcgccg cgccggtaag tgccgcccag
     1081 ccaccgatgg agtgaacctg cgtggagccg gcgaagtcga agaagttcca cttggcggcc
     1141 agccagccgc cgccccaaat ccagtgcccg acaaccggat agatggcgac ggccatgatg
     1201 aagctgaaaa cgatgaacgt gtgatacttg atccgttcgg cgaccgcgcc cgagacgatt
     1261 gttgccgccg tcccggcgaa gacgagctgg aagaagaact tggcccacag cggcacgccg
     1321 gtccagctga tcgccgagta gtcgccggcg taggcgctgc cagtggccgg gctgttatcc
     1381 gcgccgccga ccatccacaa gccgctggtt ccgatgaagc cgttgccgtt gccgaacatg
     1441 atgccccagc cgagcagcag gaagccgatc gacgtgacgg cgaacacgat gaagttcttc
     1501 gataaaatgt tgacgcagtt cttcgaccgg cacatgccgg attcaaccgt cgcgaagccg
     1561 aggttcatga agaacacgag catggccgtg accataaccc acaaggtgtc ggccatgacc
     1621 ttcaaatcca cattggtcgg cccaacggga gcaggagcag ccgtttcctt cgcttcttcc
     1681 ttggcgggct cggcagctgg agccggtgcg gcgggctcag cgaccttggg cgtttccgcc
     1741 ggggcagccg cggcaggttc ggttttcggt gcatcctggg cggtcagcga gctgcccagc
     1801 gccaggagcg cggttagcgc ggcgagcgcc gcgagtccgc atgcgatcca gattcgattt
     1861 cgactcattg gaaagggcct tgaaaaacag ggtgaaggat ctcgaacctg ctgccggcgc
     1921 gcagacacac tcgcctgcca cgacccgcgc agcaccgcta tggccggttg catccgcagg
     1981 cgtaggcctt ccgcctgcgg gcgaaccacg aggtgccgct ccccattgca agcaccatgc
     2041 cagcacttgg atatggcgcg ccggagcggc cgaccgtgcg ccgcagatga tgaaggcgca
     2101 aagacttagg gaattcgcag tgcgcttcgt ctacgctgcc gacgcgtatg cgctgcccga
     2161 tgagcgcgca ggcacggctg gatcgttggg cagcgccaag aaacgactca ggcccgcacg
     2221 gcgtcgcggg cctgagatgt tcagtcctat tagcgctacc ctttttagaa cgtcgcgatc
     2281 aggtcgaaca cgatgacctg ctggtggttc ttcgtgccgt cgtcgaaggg gacttcgttg
     2341 atcgcgttgc ggtcgccggc gtaccagtcg gcggcgaagg cggcccggcc gtaaatgttc
     2401 ggcgtgaaga agtacttggg accgaacatc aagcggtaga agctgccgtc gaagccgctg
     2461 cgggcgtagc cgcgggcgtt ggggctgccg aagctgggca acacctggcc gacgcgaaag
     2521 ccgccgtcat cgcggaacca ctcgccgttg aggccccact gcagccggca ggtctggttc
     2581 cagtagaggt aaccgttgac gccgtaccag ctggccacct cgcccggcac gaccgcatcg
     2641 tgttgccggc cgaagtcgct ttgcaggaca gccgtcacgt cgtcactgat tttgttgata
     2701 tagacgagcg tctgcagata gcgggccgtg tacccgaagc cgttgctccg caggttgacg
     2761 cccgagaagt tcggctcgtt gccaaacacg ccgacgtagg ccagcgagcg ctgctcgtcg
     2821 atcgtgagag tcgccatcgc caggccgccc gcgtgcgggt tgcccgtgtt gtcgctgctg
     2881 tcccagccgt gcgtcagagc gccgcccagc accaggtcgt cgccggcctt gtacgtcgcc
     2941 agcaccccgg tgtgagtgaa cggctcgccg tattggaagg tgtaaggcag gacggggaaa
     3001 tagttgttcg ccgtgccgat cacatagtag ccgaccggcg agtagaaccg gccgaccttc
     3061 acgctcaagt cgttgtaggc cagttcgccg tacagctgcg ggatcgcgat gccgtagaac
     3121 ttgccgttga tgccccagga ggactcgaag ccggccgacg tgttccagcg gtagctggtg
     3181 ccgcccaggg catcgacccg cgcgccccag tcccagccgc agccttcggt gttggcggtg
     3241 cggccgaagt aatagaagag ttcgttcaac tgcccttcat tggcccggtc attccaggtc
     3301 attgggccgt tgaagcgatc gaccgggctg tacggattcc agacgaatcc cgcggccagc
     3361 caccccgccc cggtccaggg gctgtcctcg cagcagggct cccaaagctt gcaggcctcg
     3421 ccgaggcatt ccagcgggca gccgcagggc caaaggctca aaaagtcgca gccgcagcag
     3481 gagtcgcacg
//
LOCUS       DASRJB010000132         7437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_321345, whole genome shotgun sequence.
ACCESSION   DASRJB010000132 DASRJB010000000
VERSION     DASRJB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321345"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFB80_03785"
     CDS             complement(<1..920)
                     /locus_tag="VFB80_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZN32908.1"
                     /translation="MSDYPKIAQLKSVAAFRARLVELGLELPVDDSPLSAAEGSPLAQ
                     PIQLGPLKVGNRWCIHPMEGWDANRDGTPSELTLRRWRNFGKSGAKLIWGGEAAAVQP
                     DGRANPNQTLATPSNKTGLAALLQQCLTAHRESFGTTNDLAVGLQLTHSGRFCKPTSG
                     DYAPRIAYHHPLLDAKFGIDSSDNSVVWTDDDLERLIDTFVAAAGLAREVGFQFVDIK
                     ACHGYLLHEFLSARTRPGRFGGDLAGRARLLLTIVSRVREACPNLHVMVRLSIFDTLP
                     FETSREIGQPMQHALPYRFGFGVSESRPLE"
     gene            complement(1143..3470)
                     /locus_tag="VFB80_03790"
     CDS             complement(1143..3470)
                     /locus_tag="VFB80_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN32909.1"
                     /translation="METMLDTTGQDAAHESLTTAQLAAVTHVDGPLLILAGPGSGKTR
                     VVTHRVAHLLNQGINGRQILALTFTNKAADEMKARLARLVPLPTVWMGTFHRFCARLL
                     RMYGSVVGLGENYSIYDTDDSKRMLAEAILDSGVELTHASPDAVQHAISWAKNNLMGP
                     DEYVPRRGHALGGIVQRVYPAYQKRLLQANAVDFDDLLLHAARLLKENPDIRARLDER
                     YRYILVDEYQDTNLAQYAIVRALSIDFPNLAVTGDPDQSIYGWRGANLSNILEFEKDF
                     PSVKVVRLEQNYRSTKAILRVADELIKHNVRRKAKRLFTENPEGAAVRLTAYPTQRDE
                     ADDIASRIAAAIRGPNRRPRDFAIFYRTNALSRQLEHALRDHVIPYQIVNGLEFYQRK
                     EIKDILAYLNLLNNPRSDVALLRIINTPTRGIGKATVDRLRDHARLKDLTLLEAARRA
                     GVVDGISKKSAVSVAKFVSMFDRLSLAVHGNIEEIVGLVLSETGYREVLENSESEEEE
                     ERLANLEELLTAAREFDVKHPEANSLEQFLEESALVADTDAWENETDRVTLMTLHAAK
                     GLEFPVVFIVACEEGLLPHDRSRLDDEKLEEERRLLFVGITRAREELALTYAQYRSFR
                     GQSCPTVASSFLMELPRHEMEVSDSVSRRMTPDWEAGFEPGAHLDDDASQESSGDDFS
                     QEIPRKSRAKPSQVQLTSGLVTGADLLAKNERPRISPNAFRHGMMVSHPSYGAGTIIA
                     LSGDGPKRTATVRFFQDNAERKFRLLHSELTPAET"
     gene            complement(3515..4093)
                     /locus_tag="VFB80_03795"
     CDS             complement(3515..4093)
                     /locus_tag="VFB80_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HZN32910.1"
                     /translation="MRQRLRLFVVLMLVSGCSAKNNGTQSISIDTPEGKVMAHGVEQP
                     STPQQVTALSGNELTSITMPATRAGEFLQTYLPNSNPENLKDYDRAFREWQTSQSPKH
                     SSEEVVQILGAYLGNRCVADLDMEWVKVNDEYGTDYAVRSKTQDVMAFPFSTVQKRVD
                     DREYDFLNGVYYLLKQKLEAGDSKLLVPAPAK"
     gene            4328..4609
                     /locus_tag="VFB80_03800"
     CDS             4328..4609
                     /locus_tag="VFB80_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HZN32911.1"
                     /translation="MSRTRIPLSRQDEQARLMKERLEMSTAEIGLTVRTTNCLEERGI
                     FTVRDLLNSSPDQLLAIANFGEKTLEEVYKALERIGFYRASKQPKAALV"
     gene            complement(4712..5650)
                     /locus_tag="VFB80_03805"
     CDS             complement(4712..5650)
                     /locus_tag="VFB80_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN32912.1"
                     /translation="MNTNRRAWLASTVAIGAAAAIPAGQAAGQAGKGEKAVKYSLNMS
                     TVRGQKLSPPDQVELAAKAGYDAIEPWINELRQYQQGGGNIDDLRKLLSDRGLAVPSA
                     IGFAEWIVNDDAKRAAGLETAKRDMELIRSIGGTHIAAPPAGATRETNLNLLTAAERF
                     RALIDVGKSLDVVPQLEVWGFSTTLSKLGETMLVATECKHSKACVLLDVYHLYKGGSD
                     FAGLRLLSGAAMNCFHMNDFPAAPPRETIKDADRVYPGDGIAPLTQILKDLLATGFRG
                     TLSLELFNPTYWKDDALNVARTGLEKMKESVAKAAA"
     gene            5925..6449
                     /locus_tag="VFB80_03810"
     CDS             5925..6449
                     /locus_tag="VFB80_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32913.1"
                     /translation="MRTSWIWAGFVAAAALAAGAAARGADGCAAAPTCGCAAPPACGC
                     DAGDACGGCGGCGGLLGNLCCLGGHHGKHHKGPRYEGLEPGFNCGCNGSYKFPVPPLY
                     TYHWPGMWQAQLMTDYHSPWRFPPLKPYVDEVPPAALGLEEVYPQLRSVSYQAEAPSA
                     GHSVFSRHLDSMPR"
     gene            6794..>7437
                     /locus_tag="VFB80_03815"
     CDS             6794..>7437
                     /locus_tag="VFB80_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32914.1"
                     /translation="MANSSWAFQRRWAAAVCTASILGGLCAANAEDAPPAESPPQTII
                     NQYAPQKVSAPASLKPVGVEPAKAADTLEQLAPVITAPQKTTPQDSATASDLPPVVTA
                     RSLPKSVLAPIVSAGVTSKLPPIVSTGDLPKHDNESPASESLPPIVNAGARPKSAPQE
                     PAAESKLPAIVTARELPKGKPQVSIHSEAAPPVARVKATPAQGRVASADFSQQPP"
BASE COUNT         1251 a         2381 c         2443 g         1362 t
ORIGIN      
        1 tattccagcg gccggctctc gctcacgccg aagccgaacc ggtaaggcag cgcgtgttgc
       61 atcggctgcc cgatctcgcg gctggtttcg aacggcagcg tgtcgaagat cgacagccgc
      121 accatgacat gcagattggg acacgcctcg cggacgcggc tgacgatcgt cagtagcaat
      181 cgagcccggc cggccaggtc gccgccaaag cgtcctggcc gcgtgcgagc gctcagaaac
      241 tcgtgcagca ggtagccgtg gcaggccttg atatcgacga actgaaagcc gacctcgcgg
      301 gccaggccgg cggcggcgac gaacgtgtcg atcagccgct ccaggtcgtc gtcggtccag
      361 acgacgctgt tgtcgctgga gtcgatgccg aatttcgcgt cgagcagcgg atggtgatag
      421 gcgatccgag gcgcgtagtc gccgctggtg ggcttgcaga agcggccgga gtgcgtgagc
      481 tgcaggccga cggccagatc attcgtcgtg ccgaacgact cgcgatgcgc cgtcaggcat
      541 tgctggagca gcgcggcgag gccggttttg ttcgatggcg tcgccagcgt ctgattggga
      601 ttcgcccgcc cgtcgggctg cacggcggca gcttcgccgc cccagatcag cttcgcgccg
      661 ctcttgccaa agttccgcca ccgccgcagc gtcaactcgc tcggcgtgcc gtcgcgattg
      721 gcgtcccagc cctccatcgg atggatgcac cagcggttgc cgaccttcag cggcccaagc
      781 tggatcggct gcgccagcgg agatccttcc gcagccgaca gcggcgagtc atcgaccggc
      841 agctccagcc cgagctccac cagccgggcg cgaaacgccg ccacgctttt gagctgggcg
      901 atcttgggat agtcggacat accgcggtgg aatcgtggaa atcggcaacc aagcgggtgg
      961 gtgcggtatg tggcgggagc cgccattcta gcagacgcgg cacggttgta acggccactc
     1021 caggcctggt tcccaagctc tgcttgggaa cccacgtctc gaagctctgc ttcgcccgcg
     1081 gcacaggaag cggagcttcc agacttgcgt tcccaagcgg agctcgggaa cgagacgcgt
     1141 gcctacgtct ccgctggtgt cagctcgctg tgcagcaggc ggaactttcg ctcggcgttg
     1201 tcctggaaga agcggacggt ggcggtgcgc ttggggccgt cgccggagag ggcgatgatc
     1261 gtcccagcgc cgtagctggg atgcgacacc atcatgccgt ggcggaaagc attgggcgat
     1321 atccttggcc gctcgttttt ggcgagcaaa tcggcgccgg tgaccaggcc gctggtgagc
     1381 tgcacctgcg atggcttggc ccgcgatttc cgtgggatct cctgcgaaaa gtcgtcgcca
     1441 ctggattcct gcgaggcatc gtcgtccagg tgcgcccccg gctcgaagcc ggcttcccaa
     1501 tcgggcgtca tccgccggct gacgctgtcg gagacttcca tctcgtgccg cggcaattcc
     1561 atgaggaacg agctggcgac ggtcgggcag gactggccgc ggaacgagcg gtactgggca
     1621 taggtcagcg ccagctcctc gcgggcccgc gtgatgccga cgaagagcag ccgccgctct
     1681 tcctcgagct tctcgtcgtc cagccggctg cggtcgtgcg gcaacaggcc ctcttcgcag
     1741 gcgacgatga acacgacggg aaactccagc cccttggcgg catgcagcgt catcagcgtc
     1801 acgcggtcgg tctcgttctc ccaggcgtcg gtgtcggcca ccagggccga ttcctccagg
     1861 aactgctcca gcgaattggc ctccgggtgt ttgacgtcga actcgcgggc ggcggtgagc
     1921 agctcctcga ggttcgccag ccgctcttcc tcttcctccg actcgctgtt ctccagcacc
     1981 tcgcgatagc cggtctcgct gagcacgagg cccacgatct cctcgatgtt gccgtgcacg
     2041 gccaggctga ggcggtcgaa catggacacg aacttcgcga ccgagacggc ggactttttc
     2101 gagatgccgt ctacgacgcc cgcccgccgg gccgcctcga gcagcgtcag gtccttcagg
     2161 cgggcgtggt cgcgcagccg atcgaccgtc gccttgccga tgccgcgggt gggcgtattg
     2221 atgattcgca gcagcgctac atcgcttcgc ggattattga gcaggttcag atacgccagg
     2281 atgtccttga tttccttgcg ctgatagaac tccaggccgt tcacgatctg gtacggaatc
     2341 acgtggtcgc ggagcgcgtg ctccagctgc cgcgacaggg cgttggtgcg atagaagatc
     2401 gcaaagtcgc gcgggcggcg attggggccg cggatggcgg cggcgatccg gctggcaatg
     2461 tcgtcggctt cgtcgcgctg cgtcggatag gccgtcagcc gcaccgccgc gccttcgggg
     2521 ttctcagtga acagccgctt ggccttgcgg cggacgttgt gcttgatcag ctcgtcggcg
     2581 acgcgcagga tcgccttcgt ggagcgatag ttctgctcca gccgcacgac cttgacgctc
     2641 ggaaaatcct tctcgaactc caggatgttg ctgaggttgg ccccgcgcca gccgtagatc
     2701 gactggtcgg ggtcgccggt gacagcgagg ttgggaaagt cgatcgagag ggcccgcacg
     2761 atcgcgtact gggccaggtt cgtgtcctgg tattcatcga cgaggatgta gcgatagcgc
     2821 tcgtcgaggc gggcgcggat atccgggttc tctttcagca gcctggccgc gtgcaggagc
     2881 aggtcgtcga aatcgacggc gttggcctgg agcagccgct tttgataggc gggatagacc
     2941 cgctgcacga tgccgcccag ggcgtggccg cggcggggca cgtattcgtc cggccccatc
     3001 aggttgttct tggcccagct gatcgcgtgc tgcacggcat cgggcgaggc gtgggtcagc
     3061 tcgacgccgg aatccaaaat cgcctcggcc agcattcgct tgctgtcgtc ggtgtcgtag
     3121 atcgagtagt tctcgcccag ccctaccacc gagccataca tccgcaagag ccgcgcgcaa
     3181 aagcggtgga acgtgcccat ccagaccgtc ggcagcggca ccagccgggc caggcgggct
     3241 ttcatctcgt cggccgcctt gttggtgaaa gtcagggcga gaatctgccg gccgttaatg
     3301 ccctggttca gcaggtgggc cacgcggtgc gtgaccacgc gggtcttgcc gctgccgggg
     3361 ccggcgagga tcaggagtgg gccatcgacg tgggtgacgg ccgccaactg cgccgtcgtt
     3421 agagattcat gagcggcgtc ttgcccggtg gtgtcgagca tcgtttccat acggttatcg
     3481 gattcgattc gtggctccct cgctccattg tgactcattt ggctggcgcc ggaacgagta
     3541 gcttgctatc acccgcctcc agcttttgct tcagcaagta gtagacgccg ttcaggaagt
     3601 cgtattcgcg atcgtccact cgcttttgca ccgtcgagaa gggaaacgcc ataacgtcct
     3661 gagtcttgct gcgcacggcg taatccgttc cgtattcgtc gttgactttg acccactcca
     3721 tgtccaaatc ggcgacgcac cggttcccca aataagcgcc cagaatctgc acaacctcct
     3781 cggaggagtg tttgggcgat tgactcgttt gccattcgcg aaaagctcga tcgtagtcct
     3841 tcaagttttc cggattggag ttgggaagat aagtttgcag aaactcccca gcgcgtgtag
     3901 cgggcatggt aatcgacgtc agctcgttgc cggaaagggc tgtgacttgc tgtggcgtcg
     3961 aaggttgctc aacaccgtga gccattacct ttccttccgg cgtgtcgatc gaaatggatt
     4021 gagtcccgtt gttcttggcc gagcagccac tgacgagcat caggaccacg aagagtcgaa
     4081 gccgttgtct catctcagcc tcctgcgcga ttgaactatt gtagggtttg cggttcagtg
     4141 gccgtctacg ctagcgtagc attgctagca cttgcgccaa aattcttcag aaataaccgc
     4201 gcaagacggt agtaaatcga aaacggttgg ttatactttc cgcccaagtt gcaagtgggg
     4261 caggcatgct tgcctgcctg gcagacagga atgtccgccc cacattccac cgctcacgga
     4321 gggagctatg agccgtaccc gcattccgct cagccgtcag gacgaacagg cccgcttgat
     4381 gaaggagcga ctggagatga gcacggccga aatcggcctc acggtccgca ccaccaactg
     4441 cctcgaagag cgcggcatct tcaccgtccg cgatctcctg aacagctcgc ccgaccagct
     4501 cctggcgatc gctaacttcg gcgagaagac gctggaggag gtctacaagg ccctggaaag
     4561 aatcggcttc taccgagcct cgaagcagcc caaggccgcg ctggtgtgac ccgcgtgggg
     4621 gaaggggtcg ggagtctttg tcgctggcga ttgtcggtgt ggagagtcga ttgccgacaa
     4681 agactcccga ccccggcatt ggcccgagag atcaggctgc cgctttggcg accgactcct
     4741 tcatcttctc caggccggtg cgggcgacgt tcagcgcgtc gtccttccaa taggtcgggt
     4801 tgaagagctc cagcgagagc gtgccgcgaa agccggtcgc gagcaggtcc ttcaggatct
     4861 gcgtcagcgg ggcgatgccg tcgccgggat agacgcggtc ggcgtccttg atcgtctcgc
     4921 gcggcggcgc ggccggaaaa tcgttcatgt gaaagcagtt catcgccgcc ccggaaagca
     4981 gccgcaggcc ggcgaaatcg ctgcctccct tgtagaggtg atagacgtcg agcaggacgc
     5041 aggccttgga atgcttgcac tcggtggcga cgagcatcgt ctcgcccagc ttgctgagtg
     5101 tcgtcgagaa gccccaaacc tcgagctgcg gaacgacgtc caggctcttg ccgacgtcga
     5161 tcagcgcgcg aaaccgctcg gcggcagtca gcaggttgag attcgtctcg cgggttgccc
     5221 cggccggcgg ggcggcgatg tgcgtgccgc cgatcgagcg gatcagttcc atatcccgct
     5281 tcgcggtctc caggccggcg gcccgcttcg cgtcgtcgtt gacgatccac tcggcgaagc
     5341 cgatcgcgct gggcacggcc agcccgcggt cggagaggag cttccgcaag tcgtcgatat
     5401 tgccgcctcc ttgctgatac tgccgaagct cgttgatcca gggttcgatg gcgtcgtagc
     5461 ccgccttggc cgccagttcg acctggtccg gcgggctcag cttctgcccg cggacggtgc
     5521 tcatgttcag cgagtacttg actgccttct cgcccttgcc agcttgccct gcggcctggc
     5581 cggccggaat tgccgccgcc gcgccgatcg ccacggtact ggccaaccac gctcgccgat
     5641 tggtattcat cgctgctgac tcctgggtcc acggctcatt gccgccattt tcaccacgct
     5701 agcgcgccac cgcaatcatg gtgggtgcca gcgacttgct gaccccgatc cccgaactct
     5761 catgtcaatt ccggcaaact cggcaagcgc cgcgctttcg ccagaggccg ttcgtttttc
     5821 gcggccggcg cggccgatga cagccacggg ccagcacgcc ggtgccggtc cctgcgcggc
     5881 cgcgctcgcg gcttgccaaa ggcgtggcca ccggagatgc aatcatgcgg acgagctgga
     5941 tctgggcggg tttcgtggct gccgcggcgc tggcggccgg agcggcagcg cgcggcgcgg
     6001 acggctgcgc cgccgctccc acttgcggtt gcgcggcacc cccggcttgc ggctgcgacg
     6061 cgggcgatgc gtgcggcggc tgcggaggct gcggtggact tctcggcaac ttgtgctgcc
     6121 tcggcggcca ccacggcaag caccacaaag gcccgcggta cgaagggctc gagcccggct
     6181 tcaactgcgg ctgcaacggc agctacaagt tcccggtgcc gccgctgtat acgtatcact
     6241 ggcccggcat gtggcaggcg cagctcatga ccgattacca cagcccgtgg cggttcccgc
     6301 cgctgaagcc ctacgtcgat gaagttccgc cggcggcgct cggcctggag gaggtctatc
     6361 cgcagctgcg atcggtctcg taccaggcag aagcgccttc ggcagggcac agcgtgttca
     6421 gccgtcatct ggattcgatg cctcgatagt aacaaaagat catatagtgg atcacgcggg
     6481 ccggcgggct gcaaacccgc cggccgcgtt cgtttcttgg cgggcatact tggcagccgg
     6541 caaggtctgc cagaatgccg cccatacagt ccctacgcgt ggaattaccg gcagcatttc
     6601 ttcaattggc cgtgcgggtt gctccgattg ctccaagggg gacgcctggc cggatcgtgg
     6661 gggccagccg ctcacgagtg gttggcggga acaggtgact tccacaggcg cgcgaattag
     6721 tttggaactt tgtcgccggc aggctcgggg aaaagccggc ggccgagtgc agcagaaagc
     6781 aaggggaacg gcgatggcaa attcgtcatg ggcatttcag cgccgctggg ctgcggccgt
     6841 ttgcacagca agcattttgg gcggactctg cgcggcgaac gccgaagatg cgccaccggc
     6901 cgagagccca ccgcaaacta tcatcaatca atacgcgccg cagaaggtct ccgcgccggc
     6961 cagcctgaag ccggtgggcg tggagccggc caaggcagcg gacacgctcg agcagctcgc
     7021 gcccgtcatc accgcaccgc agaagaccac gccgcaagac tcggccaccg ccagcgacct
     7081 gccgcccgtc gtgaccgccc gctccctccc caagagcgtc ctggcgccga tcgtcagcgc
     7141 cggcgtcacg tcgaagttgc cgcccatcgt gtccacgggc gatctgccga aacacgacaa
     7201 tgagtcgccg gcgagcgaaa gcctgccgcc cattgtgaat gccggcgcta ggccgaagag
     7261 cgcaccgcag gaaccggccg cggagagcaa actgccggcc atcgtgacgg ctcgcgagtt
     7321 gcccaaaggc aagccgcaag tttcgatcca tagcgaagcc gcgccgcccg tggcccgcgt
     7381 caaggcgacg ccggcgcaag gccgcgtggc gtcggccgat ttttcccagc agccgcc
//
LOCUS       DASRJB010000133         8383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_887085, whole genome shotgun sequence.
ACCESSION   DASRJB010000133 DASRJB010000000
VERSION     DASRJB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887085"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2942)
                     /locus_tag="VFB80_03820"
     CDS             complement(<1..2942)
                     /locus_tag="VFB80_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32915.1"
                     /translation="MQKGPESLARKVLDLAAEQGLLEPKVIAELRQRVSESKFIITPE
                     AIAKVLVDHGHLTPFQARKLVGQALGPEPEPEPRRRSHEPEEELTFADPADLAEQAAN
                     RAPAEEDEIVELEAVAPPPQPAKPAGKVSVRRQAPAAPSTAPAEPVKAKKKERAPAPA
                     VGDDDLAGLGPAEPSGKWKGESRTSASARPAAKSAVQPAAKTTAAPAAAPAGVAELDE
                     GLMPLDAMESTGAEQSAGPLAPVGKKAKKNVWDSPLILVGGGLLGVLMIAFLALLYAL
                     TRGTAAEAFNKVEEEYKSGNYGVAIEGYQRFLDQYSSNPNASLARVRLGLAQLHQVTD
                     DGRNPGLALQTAKEVLPEIEKEQKSFGEARPELAAMLPEIADGFASQAERAGETARKE
                     ELVKLAGEAMQLVNNPSYLPASLRKEREGRIAAILDKLKAAQRSIDQDKELITAIGKI
                     KGAAEKGNAREAYSVRDTLVRTYPALEVNPQLIAAIAGVGERERQLVKVASGGEPPTN
                     DEPPAAAISRVIVAVREGPATAASAGQPVFVLVEGSVYGLDGANGRVLWRRFVGYETV
                     IGPTPLPAGDSADALIVDSRSRELLRVGGATGQLVWRQALNDSATPPLVLGERVVVTT
                     RGGRVIALEAATGNVLHAAQLPQQASVAPGVGGQSRLYQLGEHSTLFVLNADTLECTE
                     TVYLGHKAGAVFVPPVSVFGMVLFVESPADDYSLVHVLAPDAKTKRLAAVGRPFRLSG
                     RVLAPPALKGRGRVAVVTDLGQIAVYEADAANAQQPVRLLAGQQASETAPLMLHLAFD
                     GNRLWTAGKRCVSLEVQGALQQLNRKWSANQDDLFIGPLHVQGETLVHCRRTPGGGAV
                     LVEGVSAETGRPQWTTHLASPIVALAVSESRRTVDVLTGEGRLFSLSAEQLRAGYVDS
                     PTFSPPPGSGGAILPEASLSPDQSQLVWTEARPGGSVYGYNVAGGGSPSSAPLPTGAQ
                     AAAAAQPLG"
     gene            complement(3093..3998)
                     /locus_tag="VFB80_03825"
     CDS             complement(3093..3998)
                     /locus_tag="VFB80_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32916.1"
                     /translation="MSAPPRPAEFHPPDSSLRVSRYDQVSGLLVTVLLALGTITLLMF
                     LVWLSSRTAWTVPAVPVTVLEDVGGGGSGNMAPAERELVEPPPEEVQDIAEPPVAASL
                     EAISTIVSVEAAQLDAIVTAVDGSTGTGHGKGSGVGDGTGPGPGGPGTSDGIPAWERW
                     EVRMTANNDVEYSKQLDFFKVELAVAGGGSPDVEYISNLSSPKPTVRRGDPKKENRLR
                     FMHRSGELRQADRRLAAKAGVKTDGRVVFQFYDQATYKALLTLENAKMGKRRIREVRR
                     TVFGVKGSGGRYEFYVIDQQYIGGA"
     gene            4151..4783
                     /locus_tag="VFB80_03830"
     CDS             4151..4783
                     /locus_tag="VFB80_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN32917.1"
                     /translation="MNVNDLAQRLGDACYGFLALNFFWGLYSVIMGWRRVRQLSFKNQ
                     EQQKAFLEELLQLLHGGKFEAAAEMCENDQRALPQLAHLAIVNRGIGFEPLRLLVTEA
                     LQRDVLGSLEYRTNWIATTIKSGPLLGLFGTVLGMMAAFGRIGTGAKIGPAQIADEIS
                     IALICTAMGLSTAIPFNFLLASIQVRVRRLQDSLAAGVTRVLDYFKRNPV"
     gene            4790..5299
                     /locus_tag="VFB80_03835"
     CDS             4790..5299
                     /locus_tag="VFB80_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN32918.1"
                     /translation="MQASSAPSSSSSAAAAGDLLGALSEREDPQRHAEFDITAMIDLV
                     FMMNIYFLVTFIGAASEEVNLPTAMHCAPLDGELATVITVVAGHDAQSVNVYVGDGPK
                     GTAITDPEQQAERITQAVEQGVAAGKTAVLIKAEKGVRLREMGRLAAAAAQEGVTLHL
                     GVMEKDVAE"
     gene            5296..5937
                     /locus_tag="VFB80_03840"
     CDS             5296..5937
                     /locus_tag="VFB80_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN32919.1"
                     /translation="MSVRFSCPQCGKQLKAREADAGRTTACSGCGTKIVIPSAEGAAP
                     DAEAAAGLPETAAGEDDEPMLPMPPRRPAEDLIDMTAMVDIVFFLLIFFLVTSLQAVL
                     AVINLPTPQAAVGTAASRSIADLTNDPDYIVVRIEDDDSIWVEDAQAFNDQEIRVKLR
                     SAADELGRPPSILVIGNADASNGAAVRVFDACAYAKVKNVSFLVQEGDVEQAP"
     gene            complement(5978..7600)
                     /locus_tag="VFB80_03845"
     CDS             complement(5978..7600)
                     /locus_tag="VFB80_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32920.1"
                     /translation="MTASWTNPHGAVLAAVALAAGLCLPGGLGAVTPESPEVKAAIGK
                     ALAFLEKQDDERLGGKCLIGLCFFKAEKGPNHPKIAAALAACEKALGAPTNEDNYSVG
                     LALIFLCEVDPSRHRLLAERYVKILLERQQEGGGWGYPGNDHGDTSQTQYPVLGLWLA
                     ANSGIDVPNTAIEKACGWLLRTQDPYGAWGYQGEDPGKYQRVPQNEVRASLGAAGLGA
                     LYISCEFLGITTAPVTAAGEAAPALPAALKVVEDAQPVARRKTYVTRVIDHALARRAM
                     TDGDNWMQKNLHGDAGQWFHYYLYALERYHSFRESALKLNDQEPKWYNEMFAKLQKEQ
                     QAAGNWEGNDNAVIATAFGTLFLLRSARKTLAHVAQRSSEGVLLGGMGLPKSTADLRE
                     RDGKIVETPFAGTVDELIALISNPDHPDFANLSDTTGAVALDSDVTKRSGQIAQLRAI
                     VTAGTVDSRLIAIRTLGRVRELDNVPVLIHAFTTNEPRLVRATDQALRFISRKFGGVG
                     LPDEPQEGDIKAAAAAWKAWYLSIRPTAEFLD"
     gene            7719..8144
                     /locus_tag="VFB80_03850"
     CDS             7719..8144
                     /locus_tag="VFB80_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZN32921.1"
                     /translation="MNFPRYKLWLIAGAIFGCLAVALGAFGAHGLELRLKESTSLSAA
                     EREHALAVWDTAAQYQMYHALALLAVGLLAARRCGLAIHLAGAALTLGTLIFSGCLYA
                     LVLTGEKWLGAVVPIGGSLLIVGWACLTVAGLKAPQSTE"
BASE COUNT         1332 a         2883 c         2718 g         1450 t
ORIGIN      
        1 cccagcggct gtgcggcggc tgccgcctga gcgccggtgg gcaagggggc ggaactgggc
       61 gagccgccgc cagccacgtt gtagccatag acgcttcccc cgggccgtgc ttcggtccac
      121 accagctgcg actgatcggg cgacaggctc gcctcgggca gtatggctcc gccactgccc
      181 ggcggcggcg agaacgtcgg cgaatcaacg taaccggcac gcagctgctc ggccgaaagc
      241 gagaacagcc ggccttcacc cgtcagcacg tcaaccgtcc ggcggctctc gctgacggcc
      301 agcgccacaa ttggcgaggc taggtgcgtc gtccactgcg gcctgcccgt ttcggcgctc
      361 accccttcca cgaggacagc gccgccgccg ggcgtgcggc ggcaatggac gagcgtttca
      421 ccctgcacgt gcagcggccc gatgaacagg tcgtcctgat tggccgacca cttgcgattg
      481 agctgctgca gcgccccttg cacctccagt gagacgcagc gcttgccagc cgtccacagg
      541 cggttgccgt cgaaggcgag gtgcagcatc agcggggccg tctcgctggc ctgctgtccc
      601 gccaagagac gcaccggctg ctgggcattg gcggcatcgg cttcgtagac ggcgatctgc
      661 cccaggtcgg tcaccaccgc cacgcgacct cgccccttca gggccggcgg cgcgagcacg
      721 cggccgctga gccgaaacgg ccggccgaca gcggccaggc gcttggtctt ggcatcgggc
      781 gctagcacgt gaaccaggga ataatcgtcc gccgggctct ccacgaatag caccatgccg
      841 aacacggaga ccggcggcac aaagaccgcg ccggccttgt ggcccagata gaccgtctcc
      901 gtgcactcca gcgtgtccgc attcaacacg aagagggtgg agtgctcgcc gagctgatac
      961 agccgcgact gcccgccgac gccgggggcg accgacgcct gctgcggcaa ctgagcggcg
     1021 tgcagcacat ttccggtcgc agcctccagg gcgatgaccc ggccgccgcg cgtcgtgacc
     1081 acgactcgct cccccagcac cagcggcggc gtggccgagt cgttgagcgc ctgccgccag
     1141 accagctgcc cggtcgcgcc gccgacgcgc agcaactcgc ggctgcggct atcgacaatc
     1201 agcgcatcgg ccgaatcgcc cgcgggcagc ggcgtcggcc cgatcaccgt ctcatagccg
     1261 acgaatcgcc gccacagcac gcgcccgttg gctccgtcca gcccatacac ggagccctcg
     1321 acgagcacga acaccggctg acccgcgcta gctgcagtag ctggcccttc gcggacggcc
     1381 acgatgacgc gcgagatggc ggcagcgggc ggctcgtcgt ttgtcggcgg ctcgccgccg
     1441 gaggcgactt tcaccagctg tcgttcgcgc tcgcctacgc cggcgatcgc cgcgatcaac
     1501 tggggattga cctccagcgc gggataggtc cgcacgagcg tgtcgcgcac gctgtaggcc
     1561 tcgcgggcgt tccccttttc ggcagcaccc ttgatttttc cgatggcggt aatcagctcc
     1621 ttgtcctgat cgatgctccg ctgggccgcc ttcagcttgt cgagaatggc ggcgattcgg
     1681 ccttcgcgct ccttgcggag cgaagccggc agataggacg ggttgttgac gagctgcatg
     1741 gcttcgcccg ccagcttgac cagctcctcc ttgcgggcag tctcgccggc ccgctcggcc
     1801 tggctggcaa acccgtcggc aatttccggc agcatcgccg ccaattcggg ccgggcctcg
     1861 ccgaacgact tctgctcctt ttcgatctct ggcaggacct ctttcgcggt ctgtagcgcc
     1921 agccccgggt tccggccgtc gtcggtcacc tggtgcagct gggccagccc caagcggacg
     1981 cgggccaggc tggcgttggg gttgctcgaa tactggtcga gaaaccgctg atagccctcg
     2041 atggcgacgc catagttgcc gctcttgtat tcttcttcga ccttgttgaa ggcctcggcg
     2101 gcggtgccgc gcgtcagcgc atagagcagc gccaggaagg cgatcatcag cacgcccaag
     2161 aggccgcctc ctacgaggat cagcggcgag tcccacacgt ttttcttcgc cttcttgccc
     2221 accggggcaa gcgggccggc cgattgctcg gcgccggttg actccatcgc gtcgagcggc
     2281 atcaagcctt cgtccaattc ggcgacgccc gccggagcag cggctggggc cgcggtagtc
     2341 ttggcagcgg gctgcacggc actttttgcg gcgggtcttg cggaggcgct tgttcgcgat
     2401 tcgcctttcc acttcccgct cggctccgcc gggcccaggc cggccaggtc atcgtccccc
     2461 acggcgggcg caggcgcgcg ctccttcttc ttcgctttga caggctccgc cggcgccgtg
     2521 ctcggggcgg ccggagcctg acgacgcaca gatactttcc cggcaggttt cgccggctgg
     2581 ggaggcggcg caacggcttc cagctccaca atctcatctt cttccgccgg cgcacggttg
     2641 gcggcctgtt ccgcgaggtc tgccggatcg gcgaaggtca gctcttcctc gggctcgtgc
     2701 gatcgccggc gaggctccgg ctcgggctcc ggcccgaggg cctggcccac gagcttgcgg
     2761 gcctggaacg gcgtcaggtg cccgtgatcg acaaggacct tggcgatcgc ttccggggtg
     2821 atgatgaact tcgactcgga gactcgctgc cgcagttcag cgatcacctt gggctcgagc
     2881 agcccctgct ctgcagccag atcgagaacc ttgcgggcca acgactcggg gcctttttgc
     2941 atgccagcgg tgtcctgctc gcgccgctcc ccgcgcccaa ggtctcgcta cggtgctcat
     3001 cgccagctag agcgcagacg agctggcaac ccagtagatt ctacttgcca cgccacgaga
     3061 acacagcagg cgcggctccg atccaagttc ggtcatgcgc cgccgatata ttgctggtcg
     3121 atgacgtaga actcgtaccg gccgccggag ccttttacgc caaaaacggt ccgccggact
     3181 tcgcggatgc ggcgttttcc catcttcgcg ttttccagcg tcaagagcgc cttgtaggtc
     3241 gcctggtcgt agaactggaa cacgacccgg ccatcggtct tgacgcccgc cttggccgcc
     3301 aggcggcggt cggcctgccg caactcgccc gagcggtgca tgaaccgcag gcgattttcc
     3361 ttcttcgggt cgccccggcg gaccgtcggc ttcggcgagg agaggttgga gatgtattcc
     3421 acatcgggac tgccgccgcc ggccacggcc agctcaacct tgaagaaatc gagctgtttg
     3481 gaatactcca cgtcgttgtt cgccgtcata cggacttccc accgctccca ggcgggaatg
     3541 ccatccgagg tgcccgggcc gccggggcca gggcccgtgc cgtcgccgac gcccgagccc
     3601 ttgccgtggc cggtgcccgt gctgccgtcc accgcggtca ctatggcatc gagctgcgcc
     3661 gcttcgaccg agacgatcgt cgaaattgcc tccagcgacg cggccaccgg cggctcggcg
     3721 atgtcctgca cctcttccgg cggcggttcg accagctcgc gctcggcggg ggccatgttg
     3781 ccgctgccgc cgccgccaac gtcttccaat accgttaccg gcacggccgg cacggtccag
     3841 gccgtgcggc tggagagcca gaccaggaac atcagcagcg taatcgtgcc cagggccaac
     3901 agcacggtga ccagcaggcc ggacacctgg tcgtagcgcg agacgcggag gctgctgtcc
     3961 ggtggatgga attcggcggg gcgaggaggg gcagacatgg cttctccatt aaagaacgga
     4021 tgcggcgttg aagcaagccg atgcttgcgg ccgccgcacc gcctagccag aatgaagccg
     4081 tcgcagcccg ccggatttgc gatctagcgc gcagggaaca gccggcggcg tccgaccgca
     4141 gcaggacaac atgaacgtca atgatcttgc ccaacgactg ggcgatgcgt gctatggatt
     4201 cttggccctg aatttctttt gggggctcta ctccgtgatc atgggctggc ggcgcgtgcg
     4261 ccagctgagc ttcaaaaacc aggagcagca gaaggcgttt ctcgaagaac tcctgcagct
     4321 cttgcacggc ggcaagttcg aggccgccgc cgaaatgtgc gaaaacgacc agcgggccct
     4381 gccgcagctg gcccacctgg cgatcgtcaa ccgcggcatc ggcttcgagc cgctgcggct
     4441 cctggtcacc gaggccctgc agcgcgacgt gctgggcagc ctggaatacc gcacgaactg
     4501 gatcgcgacg acgatcaaaa gcggtccgct gctcggcctg ttcggcacgg tgctggggat
     4561 gatggcggcg ttcggccgca tcggcaccgg ggcgaaaatc ggcccggccc agatcgccga
     4621 cgaaatcagc atcgccctga tctgcacggc gatgggcctg tccacggcga tccccttcaa
     4681 cttcctgctg gcctcgatcc aggtgcgggt gcggcggctg caggattcgc tggccgccgg
     4741 cgtgacccgc gtgctcgact atttcaagcg caatccggtg taggcggtca tgcaggcttc
     4801 gtcagctccc tcctcctcgt cgtcagcggc cgcggcgggc gaccttttgg gcgccctgag
     4861 cgagcgggag gatccgcagc ggcacgccga gttcgacatc acggcgatga tcgacctcgt
     4921 gttcatgatg aatatctatt tcctcgtcac gttcatcggc gcggcgagcg aggaagtcaa
     4981 cctgccgacc gccatgcact gcgccccgct cgacggcgaa ctggccaccg tgatcacggt
     5041 cgtggccggc cacgacgccc agtcggtcaa cgtctacgtc ggcgatggcc ccaagggcac
     5101 tgcgatcacc gatcccgagc agcaggccga gcggatcacc caggccgtcg agcaaggcgt
     5161 ggccgcgggc aaaaccgccg tcctgatcaa ggccgaaaag ggcgtgcggc tgcgtgaaat
     5221 gggccggctg gcggcggctg cagcccagga aggcgtgact ttgcacctgg gcgtcatgga
     5281 aaaggacgtg gcggaatgag cgtgcgcttt tcctgcccgc agtgcggcaa gcagctcaaa
     5341 gcccgcgagg ccgatgccgg ccggaccacc gcctgcagcg gctgcggcac aaagatcgtc
     5401 attccctctg ccgagggcgc agcgccagac gccgaggccg ccgctggcct gccggaaact
     5461 gcagctggcg aggacgacga gccgatgctg cccatgccgc cccggaggcc cgccgaagac
     5521 ctgatcgaca tgacggccat ggtggacatc gtcttcttcc tgctgatctt cttcctcgta
     5581 acttcgctcc aggcggtcct ggcggtcatc aacctgccca ccccgcaagc ggccgtcggc
     5641 accgccgcca gccgcagcat cgccgacctg acgaacgatc ccgattacat cgtcgtccgg
     5701 atcgaagacg acgattcgat ctgggtcgaa gacgcccagg ccttcaacga ccaggaaatc
     5761 cgcgtgaagt tgcgcagcgc ggccgacgaa ttgggccgtc cgcccagcat cctcgtgatc
     5821 ggcaacgcgg acgccagcaa cggcgctgcg gtgcgcgtgt tcgacgcctg cgcctacgcc
     5881 aaggtcaaga acgtcagctt cctcgtccag gaaggcgacg tcgaacaagc gccgtaatct
     5941 accgcggagt ccctcgctga cgcttcgggt tggtgtgtca atccagaaac tccgccgtcg
     6001 ggcgaatcga caaataccac gccttccacg cggcggccgc agccttgatg tctccctctt
     6061 gcggctcgtc gggcaggccc acgccgccaa atttgcggct gatgaaccgc agcgcctgat
     6121 cggtcgcgcg aactagccgc ggttcgttgg tggtgaaggc gtgaatcaag acgggcacgt
     6181 tgtccagctc gcggacgcgg cccagcgtgc ggatagcgat cagccgcgaa tcgacggtcc
     6241 ccgccgtcac gatcgcccgc agctgggcga tttgtccgga tctcttggtg acgtcgctgt
     6301 ccagagcgac ggccccggtc gtatcgctca ggttcgcaaa gtcgggatga tccgggttgg
     6361 agatcagcgc gatcagctca tcgacggtgc cggcgaacgg cgtttccacg atcttgccgt
     6421 cgcgctcgcg gaggtcggcc gtggacttgg gcagccccat gccgcccagc agcacgccct
     6481 cgctggaccg ctgggcgacg tgggccagcg tcttgcgggc gctgcgcagg agaaacagcg
     6541 ttccaaaggc cgtggcaatc accgcgttgt cgttcccttc ccagttgccc gccgcctgct
     6601 gctccttttg cagtttggcg aacatctcgt tgtaccactt cggctcctga tcgttcagct
     6661 tcaaggccga ctcgcggaag ctgtggtacc gctccagggc gtagaggtaa tagtggaacc
     6721 attggccggc gtcgccatgg aggttcttct gcatccagtt gtccccgtcg gtcatggccc
     6781 ggcgggccag cgcgtgatcg atgacccgcg tgacgtacgt cttgcggcgg gcgactggct
     6841 gagcgtcttc gaccaccttc agcgccgccg gcagcgcggg cgcggcttcg cccgctgcgg
     6901 tgaccggtgc ggtggtgatg cccaagaatt cgcagctgat atagagcgca cccaggccgg
     6961 ccgcgcccag cgaagcgcgg acttcattct gaggcacccg ctggtatttg ccgggatcct
     7021 caccctggta accccaggcg ccgtagggat cctgcgtccg cagcagccaa ccgcaggcct
     7081 tctcgatcgc cgtattggga acgtcgatgc cgctgttggc agccagccac aagcccagta
     7141 ccggatactg cgtttgcgac gtgtcgccgt ggtcgttgcc gggatatccc cagcctccac
     7201 cctcctgctg ccgctcgagc agaatcttga cgtaccgctc ggccagcaag cggtgccgag
     7261 agggatcgac ttcgcacagg aagatcagcg ccaggccgac gctgtaattg tcttcgttcg
     7321 tgggagcgcc caaggccttt tcgcaagccg ccagcgccgc ggcgatcttg ggatggttcg
     7381 gacccttttc ggccttgaag aagcagaggc cgatcaggca cttgcccccc agccgctcgt
     7441 cgtcctgctt ttccaggaag gccagggcct tgccgatcgc cgccttcact tccgggcttt
     7501 ccggcgtgac ggcccccagg ccgccgggca ggcacagccc cgcggccaag gcaaccgccg
     7561 ccagtacggc cccgtgggga ttggtccagc tggctgtcac ggcggctctc cgggtgaaaa
     7621 tcgactcgta ctagcgcgat gctaacccgg gctgctggaa gcgtcaacaa actgcgtgat
     7681 aatgcaggaa cactcgccgt atcgatttta ccgccagcat gaacttcccc cgctacaaac
     7741 tgtggctaat tgccggagca atcttcggct gcctcgccgt ggccttgggt gcgttcggcg
     7801 cgcacggtct ggaattgagg ctcaaggaga gcacttcgct gtccgcagcc gagcgcgagc
     7861 atgcgctggc agtctgggac acggccgccc agtaccagat gtatcacgcc ctggcgctgc
     7921 tggccgtcgg cctgctcgcc gcccggcgct gcggattggc gatccacctg gccggcgctg
     7981 ccctgacgct gggcacgctc attttctccg gctgcctgta tgccctcgtg ctgaccggcg
     8041 agaagtggct cggcgccgtc gttccgatcg gcggcagcct gctgatcgtg ggctgggcgt
     8101 gtctgacggt cgcggggctg aaggcgcccc aatccaccga gtgactatgc agcgtttgcg
     8161 cggtagctcg acgaggcgca cgcgcggttc ccgatgcggg tgctggcgta ttgccttatc
     8221 acgagcatta tcgcggcagc ggacacgtct ggcagcctga ccccttttct acgccctcta
     8281 ggtttggcaa gttatggtac tcggcctcgc ccgcaaacgt aaacgcgtac gttccatcag
     8341 ccaaccgcga aacgattacc gattggcccg gatcgagctt cgg
//
LOCUS       DASRJB010000134         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2577125, whole genome shotgun sequence.
ACCESSION   DASRJB010000134 DASRJB010000000
VERSION     DASRJB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577125"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFB80_03855"
     CDS             complement(<1..626)
                     /locus_tag="VFB80_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN32922.1"
                     /translation="MADSADLLALLHSGDEEAARAIFEQYAERLTRLARSRLSASLAA
                     RVDAEDIVQSAWRSFFTAARAGQFQLEHGGDLWRLLVEVTLHKLYRQAARHQAQLRSV
                     RREQALGDSAAAAAVEPTPAEAAAAADELDAVMRQLPPRGRQALALRLEGYEHAEIAE
                     QLGCHERTVRRDLAEARRVLTARAGGDFIPTAARRAPPRSLQPRREVS"
     gene            757..1035
                     /locus_tag="VFB80_03860"
     CDS             757..1035
                     /locus_tag="VFB80_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZN32923.1"
                     /translation="MAHVVTSPCFGCKYTDCVVVCPVDAFREGDQMLYIDPETCIDCG
                     LCVPECPVEAIFIDEDVPESQRDFIVLNAEMSRTCPPIYERRQPLASQ"
     gene            1083..1538
                     /locus_tag="VFB80_03865"
     CDS             1083..1538
                     /locus_tag="VFB80_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN32924.1"
                     /translation="MTKPVPKPIDPAQVRESALAVIAADRFPMLASIDGDQPRLRPVS
                     PVRTDGFVVYVANLRLYHKTAEIAANPRVELCYMDDGHNQVRLTGLAEIVTERPLLEE
                     IWNANPLMRQFLGSLDNPQLIIYRIRPTQVRYMQEWALEYWQVPLEATA"
     gene            complement(1535..>2530)
                     /locus_tag="VFB80_03870"
     CDS             complement(1535..>2530)
                     /locus_tag="VFB80_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32925.1"
                     /translation="GPAKLFMNTGFPAPGRPALGAWAIYGLGSESKSLPGFVVLQSGP
                     RGPRGGASLWASGFLPTSFQGVPFRSKGDPILNLKSPEGLTPSAERDFYDTVGQLNQQ
                     RLKQTGDPEILTRINAYEMAFRMQTSSPELMDLSGESAETLDLYGAEAGKPSFATNAL
                     LARRLVERGVRFVQLYHTNWDSHGGPGENLNDDFEKVCKDADRPSAALVLDLKRRGLL
                     KDTLVVWGGEFGRTPMLQGTDADDNPAARPGRDHHKDAFSIWMAGGGVKSGFTYGRTD
                     DLGYTITEDPMHINDFHATLLHLLGLDHERLTFKFQGRQYRLTDVAGKVATKLLA"
BASE COUNT          407 a          843 c          855 g          425 t
ORIGIN      
        1 tgcgaaactt cgcggcgcgg ctggaggctg cgcggcgggg cgcggcgggc cgcggtgggt
       61 atgaaatcgc cgccggcgcg ggccgtcagc acgcgccgcg cttcggccag gtcgcgccgc
      121 acggtccgct cgtggcagcc gagctgttcg gcgatctccg cgtgctcata gccttccagc
      181 cgcagggcca gcgcctgcct gccgcgcggc ggcaactgtc gcatcactgc atccagctcg
      241 tccgccgcgg cagccgcttc ggccggcgtg ggctcgaccg ccgcggcggc tgccgagtcg
      301 ccaagagcct gctcgcggcg aaccgagcgc agctgagcct gatggcgggc cgcctggcgg
      361 tagagtttgt gcagcgtgac ctccaccagc agccgccaca aatcgccgcc gtgctcgagc
      421 tggaactgac cggcgcgggc ggccgtaaaa aagctccgcc aggccgattg cacgatgtcc
      481 tcggcgtcca cccgcgccgc caaagacgcc gacagccggc tgcgggccag ccgcgtcagc
      541 cgctcggcgt attgctcgaa gatggcccgc gcggcttctt cgtcgcccga atgcagcagc
      601 gcgagcaggt cggccgagtc agccatgctg gcaagtctat ggcggagcac gagttgcgag
      661 aagtcgcgaa aatcgcggac cgcgttgtcc ggtttgccgc ggcaatctgc atgaactagt
      721 agcggcggct tcccgccgga aatgaggaga gtgcctatgg cccacgtcgt caccagtccg
      781 tgctttggct gcaaatacac cgattgcgtc gtcgtttgcc cagtcgatgc gttccgcgag
      841 ggggaccaga tgctgtacat cgaccccgaa acctgcatcg actgcggcct gtgcgtcccg
      901 gaatgccccg tcgaggcgat cttcatcgat gaagacgttc ccgaaagcca gcgcgatttc
      961 atcgttttga atgccgagat gtccaggact tgtccaccca tctatgagcg ccgtcagccg
     1021 ctcgcctcgc agtagccgcg ttcgattgac gacgccggcc agtcggccga aaatccaggc
     1081 cgatgaccaa accggttccc aagccgatcg atcccgcgca ggtccgcgaa tcggccctgg
     1141 ccgtgatcgc cgccgaccgc tttcccatgc tggcgtcgat cgacggcgat cagccgcggc
     1201 tgcggccggt ctcgcccgtg cgaacggacg ggttcgtggt ctacgtcgcc aacctgcggc
     1261 tgtaccacaa gaccgccgag atcgcggcca atccgcgggt cgagttgtgc tacatggacg
     1321 acgggcacaa ccaggtccgc ctgaccgggc tggccgagat cgtcaccgag cggccgctgc
     1381 tcgaggaaat ctggaacgcc aacccgctga tgcggcagtt cctcggcagc ctcgacaacc
     1441 cgcaactcat catctatcgc atccgcccca cgcaagtccg ctacatgcag gagtgggcgc
     1501 tggaatactg gcaggttccg cttgaagcga ccgcctaggc cagcagcttg gtggcgacct
     1561 tgccggcgac gtcggtgagc cggtactggc ggccctggaa cttgaacgtc agccgctcgt
     1621 ggtcgaggcc gagcaggtgc agcagcgtgg cgtggaagtc gttgatgtgc atcgggtcct
     1681 cggtaatcgt gtaaccgagg tcgtcggtgc ggccgtaggt gaagccgctc ttcacgccgc
     1741 cgccggccat ccagatgctg aaggcgtcct tgtgatgatc gcgtccgggc cgcgccgcgg
     1801 ggttgtcgtc ggcgtcggtc ccctgcagca tcggcgtgcg gccgaattcg ccgccccaga
     1861 cgaccagtgt gtccttgagg agtccgcggc gtttgaggtc gagcaccaga gcggcgctgg
     1921 gtcgatcggc gtccttacac accttttcga aatcgtcgtt gagattttcg ccggggccgc
     1981 cgtggctgtc ccagttggtg tggtagagct ggacgaagcg gacaccgcgc tcgacgaggc
     2041 ggcgggcgag caaggcgttg gtggcgaacg agggcttgcc ggcttcggcg ccgtagagat
     2101 cgagcgtttc ggccgattcg ccggagaggt ccatcagctc ggggctgctg gtctgcatgc
     2161 ggaaggccat ctcgtaggcg ttgatccgcg tgaggatctc ggggtcgccc gtctgcttca
     2221 gccgctgctg gttgagttgg ccgaccgtgt cgtaaaagtc gcgctcggcc gacggcgtca
     2281 gcccttcggg actcttcaaa ttcaggatcg gatcgccctt gctgcgaaaa ggcacgccct
     2341 gaaacgaggt gggcagaaag ccgctggccc acaacgacgc gccgccgcgc ggcccgcgcg
     2401 gacccgattg caagacgacg aaaccaggca gactcttcga ttcgctcccc aggccgtaga
     2461 tcgcccaggc ccccagtgcc ggccggcccg gcgcgggaaa gcccgtgttc atgaacagct
     2521 tcgcgggccc
//
LOCUS       DASRJB010000135         7251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2295524, whole genome shotgun sequence.
ACCESSION   DASRJB010000135 DASRJB010000000
VERSION     DASRJB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295524"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFB80_03875"
     CDS             complement(<1..612)
                     /locus_tag="VFB80_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZN32926.1"
                     /translation="MANHPDVFQRLVGLETEYALHSSASGGQQLPSRYALFSEVLAAL
                     KRKIPCARARTMKEGAFHAAGGAVWFETERPALGGGLIEGATPECRSPRQVLAWQRAQ
                     DELLAEAASAAFGGALKLIKNDRDAAGNVYGAQENYDAEFAAGWRLVAWRTALVLLAP
                     LAIVTWIVLWMLDLLAAGYAIVATLIYLTAERFLPRPEWLARLL"
     gene            complement(617..790)
                     /locus_tag="VFB80_03880"
     CDS             complement(617..790)
                     /locus_tag="VFB80_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32927.1"
                     /translation="MNSPAVPSPANSHVKPAAPVRPLDPRAVAAVLAGIRTDSQREPE
                     TFLRDTEVPYGGE"
     gene            complement(919..1353)
                     /locus_tag="VFB80_03885"
     CDS             complement(919..1353)
                     /locus_tag="VFB80_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HZN32928.1"
                     /translation="MSQFPSQPGAPNPFAASPGFQQPPKKSRAWLWILLGVGGTMLVV
                     CCGCVGLFALGWNEIGRQITAQLNADPTAQQHLGTVSSATMDLVATGEEAQQNPGKNE
                     QRLVFQVKGDKGSGKVSAKQVPGGFNDATLILPSGERIKLGF"
     gene            1609..2355
                     /gene="recO"
                     /locus_tag="VFB80_03890"
     CDS             1609..2355
                     /gene="recO"
                     /locus_tag="VFB80_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZN32929.1"
                     /translation="MASEKTRAIVLRLVEFSETSLVVTLFTEDFGKVGALAKGARRPK
                     SPLEGALDLLALIRIVFLRKSSESLDLLTEARLERRFRSAERELARLYAGYYVGELLS
                     ELTDTGDPHPALFAAGDQTLAAIDRGEPLAETVLRFELAALKLVGHWPSLAACVGCGR
                     PVDGGEERVPLSMISGGVLCGDCRPGRRGVVGVSSAVLAALRTAGEEQQPLPESWSLA
                     PDVRGELRAVMNNYLTHLVGHRLRMPPYLQ"
     gene            2423..3688
                     /gene="bamD"
                     /locus_tag="VFB80_03895"
     CDS             2423..3688
                     /gene="bamD"
                     /locus_tag="VFB80_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZN32930.1"
                     /translation="MLVLVSGCASLQPGGGESASSRTAAPGEAQPASYEEPAENKLPF
                     EDFSIDNLGKTVKKLTGNGPNREVAKQIFREADDIFRQALAAEPEKRAQIFEIAAPKY
                     AAAAERWPDSALAMDALFSAGECYFFADNYPQASAQYEKLVKAFPNNRYQDTVDQRRF
                     LIAKYWLALHHESPEPIYYYNWFNKSRPWRDARGNGLRIFDKIRIDDPTGRLADDATL
                     AAGNEHFLAGKFYKADEYYSDLRKAYPTSEHQFLAHFVGLKAKLNCYQGPEYGGTLLD
                     EAEKLVKQIRRQFPQEAQQEREFLDRAAAEIRYLKAQRLMFLAEYYDQRSQYGAAHHY
                     YDQVAQQFSDTPLAQRAGARIGQIAGLPAKPAQVAPWLVGMFPESDKVKPLLAATERA
                     RQAGAQQGTEQAAPDEQRLADRPAATANY"
     gene            3738..4247
                     /gene="lptE"
                     /locus_tag="VFB80_03900"
     CDS             3738..4247
                     /gene="lptE"
                     /locus_tag="VFB80_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZN32931.1"
                     /translation="MQFAICILLFLPGCAGYQIGQRSLYRPDIRTIHVPVVYSDSFRR
                     HLGERLTEAIVKEIELKTTYKVVDADSADSVLSVRLASDSKRILAENRFDEPRDIETD
                     LFAQISWVDRRGDLIMSHDDVPAAPLLLNIGQSANFVPEGGQSLATAQQETIQRMAEQ
                     IVGQMELAW"
     gene            4419..6503
                     /locus_tag="VFB80_03905"
     CDS             4419..6503
                     /locus_tag="VFB80_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN32932.1"
                     /translation="MEAQGPSGDLLSPSRANVLLVDDIPANLLALRVTLEDLGQNLVE
                     ASSGQEALAHLQTDEFAVILLDVLMPGMSGFETAKHIRASDRSRHTPIIFLTASDIEP
                     PELEEAYSLGAVDFLVKPLLPLILQGKVRGLVELFHDKQRARIQGDQLQLLVQGTTEY
                     AIFMLDPDGRVVTWNTGAERLKGYKAEEIIGEHFSRFYPPDANEKDWPKHELEVARSK
                     GRFEDEGWRVRKDGTRFWANVVIAALRDAAGTLRGFSKITRDMTERKQSEENARRLVA
                     EAAARRAAEENARLIQEQRERLRVTLASIGDAVISTDAEGHITFVNPIAENLVGWNSD
                     DAAGRTLAEVFRIINEDTRQPVENPAMRALRQGAIVGLANRTVLIARDGTERPIDDSA
                     APIRDAAGKVIGSVLVFRDITERRRNEARVREQQEWLRVTLASIGDAVIAADKQGCVT
                     FLNGVAEKLTGWTQQDAKGQRLEAVFPILNEQTRMPVENPVARVLRDGAVVGLGNHTV
                     LSGKDGTERPIDDSAAPIRDAAGKLIGVVLTFRDVTEQRRISSALRQSEAESRRMLEF
                     HEAVMQNMGEGLYAVDTQGLVTYMNPAAERLFGWRSSELLGRKMHEMTHYKHPDGSPF
                     PIEECAGFRALGEGKVLRNHDDVFIRKDGTFFPVVYSSSPLVTQGRVCGLVVVFREAT
                     RAPPNQRRRLIS"
     gene            7015..>7251
                     /locus_tag="VFB80_03910"
     CDS             7015..>7251
                     /locus_tag="VFB80_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32933.1"
                     /translation="MSATLDDYQWLVSDAAQPWLTRLQPELERLATPAMLQRLRKDLS
                     AERVHLVVEQVELRQRAREKFSLADRMFFTRKGLE"
BASE COUNT         1344 a         2279 c         2372 g         1256 t
ORIGIN      
        1 gagcagccgg gcgagccatt ccggccgcgg caggaagcgt tcggcggtca gatagatgag
       61 tgtggcaaca atggcgtagc ccgcagcgag cagatcgagc atccacagca cgatccaggt
      121 cacgatcgcc agcggcgcca gcagcaccag cgccgtccgc caggcgacca agcgccagcc
      181 ggcggcgaac tcggcgtcgt agttttcctg cgcgccgtac acgttgcccg cggcgtcgcg
      241 gtcgttcttg atcagcttga gcgcgccgcc gaacgcggcg ctggcggctt cggccagcaa
      301 ttcatcctgg gccctctgcc aggcgagcac ctgccggggc gagcggcatt cgggcgtcgc
      361 gccttcgatc agcccgccgc ccagggcggg ccgctcggtc tcgaaccaga ccgcgccgcc
      421 ggccgcgtga aaggcgcctt ccttcatcgt ccgcgcgcgg gcgcagggga tcttccgctt
      481 cagggccgcg aggacctcgc tgaacagcgc ataacggctg ggcagctgct ggccgccgct
      541 ggccgacgag tgcagcgcgt attcggtctc cagccccacg aggcgctgga agacgtcggg
      601 atggttcgcc actgatttat tcgccgccgt aaggcacttc cgtgtcgcgc aggaaggtct
      661 cgggctcgcg ctggctgtcg gtgcgaatgc ccgccaggac cgcggccacg gcccgcgggt
      721 cgagcggacg aaccggggcg gcaggcttca cgtgactgtt ggcgggcgag ggaacagcgg
      781 gggagttcat tgcgggggca cccaacgcga gcggagtagg gctgccggcg gatgaggccg
      841 gcgtggattc atctatggta gtcctcggcc gccgcggcag caagcgcggc gcggcattcg
      901 ccgaattccc gcagccgatc agaacccgag cttgatccgt tcgccgctgg gcaagatcaa
      961 cgtggcgtcg ttgaatccgc cgggcacttg cttggcgctg actttgccgc tgcctttatc
     1021 tcccttgacc tggaacacca gccgctgctc gttcttgccg gggttttgct gcgcctcttc
     1081 gccggtcgcg acgaggtcca tggtggccga gctcacggtg cccaggtgtt gctgcgcggt
     1141 cgggtcggcg ttgagctgcg cggtgatctg ccggccgatc tcgttccagc ccagcgcgaa
     1201 cagcccgacg cagccgcagc agacgaccag catcgttccg ccgaccccca gcaggatcca
     1261 cagccaggcc cgcgacttct tcggcggctg ctgaaacccc ggcgaagctg cgaacgggtt
     1321 gggcgcgccc ggctgactgg gaaactgcga catggatggc gtcctcggga tgcgattggc
     1381 ggcagtatgg tcgtttatct tagggtccgc tggctctcga tgcagcaacg ggaccgcgga
     1441 tttcggggaa cggcggaacc gcggattcgg cggaggaaga accgcggatt tcgcgaatgg
     1501 cgcggatgag agtgaagact tgcatgcctt atctgcgtca tccgcgttat ccgcggttca
     1561 ttccgcttcc gcggtccttg ccatccgcca gcaagatacg atgaaaggat ggcgagcgag
     1621 aaaacgcggg cgatcgtctt gcggctggtg gagtttagcg agacgagcct ggtggtgacg
     1681 ctgttcaccg aggatttcgg caaggtcggg gctttggcga agggtgcgcg gcggccgaag
     1741 agcccgctgg agggcgctct tgacctgctc gcgctgattc gcatagtgtt cctccgcaaa
     1801 tcgtccgaat ccctcgacct gctgaccgaa gccaggctcg agcggcggtt tcgcagcgcc
     1861 gagcgcgagc tggcgcgtct gtacgccggc tactacgtgg gcgagttgct ctccgaactg
     1921 acggacacgg gcgacccgca tcccgcgctg tttgccgccg gcgaccagac gctggccgcg
     1981 atcgaccgcg gcgagccgct ggccgagacc gtgctgcggt tcgagctggc ggcgctaaag
     2041 ctggtggggc attggccctc gctggcggct tgcgtcggct gcgggcggcc ggtggatggc
     2101 ggcgaggagc gcgtgccgct ctcgatgatc agcggtggcg tcttgtgcgg cgactgccgg
     2161 ccgggacgcc gcggcgtggt cggcgtcagc tcggcggtgc tggcggccct ccgcaccgcc
     2221 ggcgaggagc agcagccgct gccggagagc tggtcgctag ctccggacgt gcggggcgaa
     2281 ttgcgagcgg tgatgaacaa ctacctgacg cacctggtcg gccaccggtt gcgaatgccg
     2341 ccgtatttgc agtgatttgg tgcttcgcag catgacacgg atgtcgagcc ccaccctgcg
     2401 accatctcac gtagccagcg ccatgttggt gctcgtcagc ggctgcgcta gcttgcagcc
     2461 ggggggcggg gaatcggctt ccagccgaac tgcggcgccc ggcgaggccc agccagcgtc
     2521 gtacgaggaa cctgccgaaa acaagctgcc gttcgaggac ttttcgatcg acaacctggg
     2581 caagacggtc aaaaagctga ccggcaacgg acccaatcgg gaagtcgcca aacagatctt
     2641 ccgcgaggcg gacgacatct tccgccaggc cctggccgcc gaacccgaga agcgggcgca
     2701 gatcttcgaa atcgccgcgc ccaagtatgc cgccgccgcg gaacgctggc ccgactcggc
     2761 cctggcgatg gacgcgctgt ttagcgcggg cgaatgctac ttcttcgccg acaactatcc
     2821 gcaggccagc gcccagtacg agaagctggt caaagcgttt cccaacaacc gctatcaaga
     2881 caccgtcgat cagcggcggt tcctgatcgc caaatactgg ctcgcgctcc atcacgagag
     2941 tcccgagccg atctactact acaactggtt caacaagtcg cggccctggc gcgacgcccg
     3001 gggcaacggc ctgcggattt tcgacaagat tcgcatcgac gacccgaccg gccgcctggc
     3061 cgacgacgcg acgctcgccg ccggcaacga gcacttcctg gccggcaagt tctacaaggc
     3121 cgacgagtat tacagcgacc tgcggaaggc ctatcccacc agcgagcacc agttcctcgc
     3181 gcactttgtc ggcctgaagg ccaagctgaa ctgctaccag ggaccggagt acggcggcac
     3241 gctgctcgac gaagccgaga agctcgtcaa gcaaatccgc cggcagttcc cccaggaagc
     3301 ccagcaggaa cgcgagttcc tcgaccgggc cgccgccgaa atccgctacc tcaaggccca
     3361 gcggctgatg ttcctggccg agtattacga ccagcggtcg cagtacggcg cggcccacca
     3421 ctattacgac caggttgcac agcagtttag cgacacgccc ttggcccagc gggcgggcgc
     3481 tcggatcggc cagatcgccg gattgcccgc caagccggcg caagtcgccc cctggctcgt
     3541 cggcatgttc cccgaaagcg acaaggtcaa gccgctcctc gccgccacgg agcgagcgcg
     3601 gcaagccgga gcgcagcaag gcacggagca agctgccccg gacgagcagc ggctggccga
     3661 ccgccccgct gccacggcga actactaggt ccagcgcgga gcgggcaatg aggaccggga
     3721 acccatcaat ttgcaatttg caatttgcaa tttgcatttt gctgttcctc cccggctgcg
     3781 ccggctatca gatcggccag cgctcgctct atcggccgga catccgcacg atccacgtgc
     3841 cggtcgtcta ttccgacagc ttccgccggc acctgggcga gcggctgacc gaggcgatcg
     3901 tcaaggagat cgagctgaag acgacctaca aggtcgtcga tgccgactcg gccgacagtg
     3961 tgctctccgt gcggctggcc tccgacagca agcggatcct ggccgagaac cgcttcgatg
     4021 agccgcgaga catcgagacc gacctgttcg cccagatcag ctgggtcgat cgccgcggcg
     4081 acctgatcat gagccacgac gacgtccccg ccgcgccgct gctcctcaac atcggccaat
     4141 cggccaattt cgttcccgaa ggcggacaat ccctggccac cgcccagcag gaaaccatcc
     4201 aacgcatggc cgagcagatc gtcgggcaga tggagctggc gtggtgacct tcactcgatc
     4261 atctagcatg tctggggctg ccgcttcagc agcgctggcc ccgccgtctg gctctgttgg
     4321 acaccttggc cttcgctaaa atggtcacgc cgcatcgaga ctcccgcaac ggcattgccc
     4381 gataagcggg tactcgaagg caggacaagg ccgattccat ggaagcccaa ggcccgtccg
     4441 gagacctgct ctctccgtcg agagcaaacg tgctcctggt ggatgacatc ccggccaatc
     4501 ttctcgccct acgagtgacc ctcgaagacc tcggccagaa cctcgtggaa gcgagttctg
     4561 gccaggaagc gctcgcgcac ctgcaaaccg acgagttcgc cgtcatcctg ctcgacgtgc
     4621 tgatgcccgg catgagcggc tttgagacag ccaaacatat ccgggccagt gatcggtctc
     4681 gacacacacc catcattttt ctcaccgcca gcgatattga acctccggaa ttggaagagg
     4741 cgtattctct cggggcggtg gatttcttgg tgaagccact gttgccgctc attctccagg
     4801 ggaaggtgcg gggactcgtt gaactatttc acgacaaaca acgagcgagg attcaaggcg
     4861 atcagctaca gctgcttgtt cagggaacga cggagtacgc gatcttcatg ctcgatccgg
     4921 acggccgtgt tgtcacctgg aacactggcg cggagcgact caaagggtac aaggctgaag
     4981 aaatcatcgg cgagcatttt tctcggttct atccgcctga tgccaacgag aaggattggc
     5041 ccaagcacga attggaagtc gcgcgctcca aggggcggtt cgaagacgaa ggctggcggg
     5101 tccggaagga cggcacgcga ttctgggcca atgtcgtcat cgcggcacta cgggacgcag
     5161 ccgggacact gcgcgggttc tccaaaatca cgagggacat gacggaacga aagcaatccg
     5221 aggagaatgc ccgtcgcctt gtggcagaag cagcggctcg tcgggctgcg gaggagaatg
     5281 cgcggctcat tcaggagcag cgggagcgac tgcgagtgac gctggccagc atcggagatg
     5341 cagtgatcag caccgatgcc gaagggcata tcacgtttgt caaccccatc gctgagaact
     5401 tggtcggctg gaattcggac gatgcggcgg gacgaacact ggccgaagtg ttccgcatca
     5461 tcaatgagga cacgcgccag ccggttgaaa accctgcgat gcgagcactc cggcaaggtg
     5521 cgatcgtcgg gcttgcaaac cgcaccgttc tgatcgcgag ggacggaacg gagcgcccga
     5581 ttgacgattc cgccgctccc atccgggacg ctgcgggcaa ggtcatcggc agtgtgctgg
     5641 tctttcggga cattactgaa aggaggcgga acgaggcgcg ggtgcgcgag cagcaggagt
     5701 ggctgcgggt gacgctcgct agtattggcg atgcggtgat cgctgccgac aaacaaggat
     5761 gtgtgacgtt tttgaacggc gttgccgaaa aattgacggg ttggacgcag caagatgcga
     5821 aagggcaacg cctcgaagcg gtattcccca ttctcaatga gcagacccga atgccggtcg
     5881 agaacccggt cgcgagggtg ctgcgggacg gagccgtcgt tggcttggga aaccatacgg
     5941 tcctaagcgg taaagacggg accgagcggc cgatcgacga cagcgccgct ccaatccggg
     6001 atgccgcggg caaattaatc ggcgtcgtgc tcaccttcag agatgtcacc gagcagcgtc
     6061 gaatatcgtc ggcattgcgg cagagtgaag cggagtctcg ccgcatgctg gagttccatg
     6121 aggccgtcat gcaaaacatg ggggaggggc tgtacgccgt cgatacacag ggattggtaa
     6181 cgtacatgaa cccagctgcg gaaagactct tcgggtggag gagcagcgag ctcctgggcc
     6241 gcaagatgca cgagatgacg cattacaagc accccgacgg cagccccttc ccgatcgagg
     6301 agtgcgcagg gtttcgagct ctcggcgagg gaaaggtgct gcggaaccac gacgacgtgt
     6361 ttatccgaaa ggatggcaca ttttttcccg tcgtgtatag ctcgtcgccg ctcgtcacgc
     6421 agggccgggt ctgcggcttg gtagtcgtct tccgcgaggc gacccgggcg ccgccaaatc
     6481 agaggcgacg cctgatcagt tgaagtgatc ttcggcggtt ctattagggt gagggtattc
     6541 actccggcga attgacactt gtctacttct gcgtataatt ttctaattgg aatcctcgcc
     6601 gcggatgcgg tgagcgacgt tctttgaaaa ttcatctctg acgtcagcgg ctggcgcatt
     6661 tgcagcgcgc ataatgcgca tattcatgcg cgctcaagaa tcgacgtaac ccgcttcgcg
     6721 gcagccactt gcggccattt ttggcgtccg cagctttttc cgcgttttgc gcgcatagcg
     6781 gccacgcgtg ctggcggctc gttaaccagt agttggacgc gcgggctttt tccgcgagga
     6841 cgccgtcgcc tctggctccg ggttaaacga acggcggcaa gccctagcgg tgcccggcgc
     6901 gcataatgcg cataatcgct ggagtcgaga aacggtcgca agtcttgccg cagcaggcag
     6961 ttgcgtccgt tttcctggcc caagtctttt ggttcgatat gcgcgcatag tcccatgtcc
     7021 gccaccctcg acgactacca gtggctcgtg tccgatgcgg ctcagccgtg gctgacacgc
     7081 ttgcagccgg agctggagcg cctcgcgacg ccggcgatgc tccagcggct ccgcaaggac
     7141 ttgtccgccg agcgagttca cctcgtcgtc gagcaagtcg agctccgcca gcgggcccgc
     7201 gaaaagttct cgctcgccga ccggatgttc ttcacacgca aagggctgga g
//
LOCUS       DASRJB010000136         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3142102, whole genome shotgun sequence.
ACCESSION   DASRJB010000136 DASRJB010000000
VERSION     DASRJB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142102"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFB80_03915"
     CDS             complement(<1..800)
                     /locus_tag="VFB80_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN32934.1"
                     /translation="MERPAYVRDLGDLLGRGDIDGFVRRCQENIQLVEGQLADAYPET
                     AYVREDLARAYYELDDYFSAESQFRRALEIHRQHGQVVSDDAARCLGMLGRLALWTGR
                     TAEAELLFCEALTLLDHVPADKQTSRGFVLIDLAQLHTWEGEYETAERLLHEAARQHL
                     RSMGYCPGRLAFVYLHLSRAYLGQGRTSAADKTLNKAIRYCIRGREVESANFAALLTE
                     RGLLLERQQKVTNARGWHSAALRILQRVRPHGCKLLERVQGRLARLAET"
     gene            complement(812..2230)
                     /locus_tag="VFB80_03920"
     CDS             complement(812..2230)
                     /locus_tag="VFB80_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32935.1"
                     /translation="MLSVSRRHFLHDCGIGLGKIAAASLLAGSAARVSAAPVQPSPLA
                     PRQPHFPAKAKAVIHLFMAGAPSQLDLFDYKPKLAEYEGKPIPPEVVGSQRYAFIRPD
                     AAVLGPRFKFSRYGQCGAELSEMLPHLAKVVDEIAIVKSVRTDQFNHAPAQLFLNSGF
                     AQPGRPCLGSWVLYGLGAETSELPAFVVMSTGSGISGGAANWSSGFLPTVYGGVRLRN
                     TGDPILDVTSPAGIDPRAQRDTLDLVGELNRQRLAALGDAEIGTRIASYEMAYRLQTS
                     APELMDLKQEDQKTLDLYGIDPAKPSFARACLLARRMVERGVRFINIYNEGWDAHSDV
                     SGNLKKNCGATDQGAAALVADLKQRGMLDETLVIWGGEFGRTPMVETNTALGRSLGRD
                     HHPQAYTMWLAGGGIRPGLTYGKTDDLGFHVVENPVHTHDLQATILHVLGLDHEKLTF
                     RHAGRDFRLTDVHGHMVMDILA"
BASE COUNT          378 a          736 c          743 g          415 t
ORIGIN      
        1 gtctcagcga gtctcgccag tcggccttgg acgcgctcga gcaatttgca cccatgaggt
       61 cggactcgtt gcaggattcg caaggcagct gaatgccaac cgcgcgcatt cgttactttc
      121 tgctgccgct cgagtaagag acctcgctcc gtgagcaatg cggcaaaatt agcactctca
      181 acttccctac cgcgaatgca ataccggatc gccttgttta gcgtcttgtc ggccgcggag
      241 gtcctgcctt gtcccaagta ggcgcgactg aggtgcaagt agacgaacgc gagacgaccg
      301 gggcagtagc ccatgcttcg caagtgttgc cgggccgctt cgtgcaacaa gcgctcggcc
      361 gtctcgtact ctccttccca ggtgtgcagc tgcgcaagat cgatgaggac gaaaccgcgc
      421 gacgtctgct tgtcggctgg cacatgatcg agcagggtca gcgcctcgca gaacaagagt
      481 tcggcctccg cagtccgtcc cgtccagagt gccagccggc ccagcattcc caagcacctc
      541 gccgcgtcgt cgcttaccac ttggccatgc tggcggtgga tttcgagtgc ccgccggaac
      601 tgcgattccg ctgagaaata atcgtcgagc tcatagtacg cccgggccaa gtcctcgcgc
      661 acgtaggccg tttccgggta ggcgtctgcc agctgccctt cgacgagctg aatattctcc
      721 tggcagcggc gcacgaaacc gtcgatgtcc ccgcggccga gcaggtcgcc gagatccctg
      781 acatatgcag gtcgctccat gttgctacag tctaagcgag gatgtccatt accatatgac
      841 catgcacgtc ggtcaggcgg aagtcgcggc cggcgtggcg gaaggtgagt ttttcgtgat
      901 cgaggccaag aacgtggagg atggtggctt gcaggtcgtg cgtgtggacg gggttctcga
      961 cgacgtggaa gccgaggtcg tcggtcttgc cgtaggtcag gccggggcgg atgccgccgc
     1021 cggcgagcca catcgtgtag gcctgcggat ggtggtcgcg gccgagcgag cggccgaggg
     1081 ccgtgttggt ctcgaccatc ggcgtgcggc cgaactcgcc gccccagatg acgagtgtct
     1141 catcgagcat cccccgctgc ttcaaatcgg cgaccagggc cgccgcgccc tgatcggtcg
     1201 cgccgcagtt cttcttcaag ttgcccgaga cgtcgctgtg ggcgtcccag ccttcgttgt
     1261 agatgttgat gaaccgcacg ccccgctcca ccatccgccg ggccagcagg caggcccggg
     1321 cgaacgacgg cttcgccgga tcgatgccgt acaggtcaag cgttttctgg tcttcctgtt
     1381 tcagatccat cagctccggc gcggaggtct gcagccggta ggccatctcg tacgaggcga
     1441 tccgcgtgcc gatttccgcg tcgccgagtg ccgccagccg ctggcgattg agctcgccga
     1501 cgaggtcgag cgtgtcgcgc tgggcccgcg gatcgatgcc cgcggggctg gtgacgtcga
     1561 ggatcggatc gcccgtgttc cgcagccgca cgccgccgta aaccgtcggc agaaagccgc
     1621 tggaccagtt ggccgccccg ccgctgatgc cgctgcccgt gctcatgacg acaaacgccg
     1681 gcagctcgct cgtttcagcg ccgaggccgt agagcaccca cgatcccagg cagggccgtc
     1741 ccggctgcgc gaagcccgag ttgagaaaga gctgggccgg ggcgtggttg aactgatcgg
     1801 tgcggacgct tttgacgatc gcgatctcat ccacgacctt ggccaggtgc ggcagcatct
     1861 ccgacagctc cgcgccgcat tggccatacc ggctgaactt gaaccgtggc ccgagcaccg
     1921 ccgcatccgg gcggatgaag gcgtatcgct gcgagccgac gacctctggc ggaatcggct
     1981 tgccctcgta ttccgccagc ttgggcttgt agtcaaacag gtcgagctgg ctcggcgcgc
     2041 cggccatgaa caggtgaatc accgccttgg ccttcgccgg aaagtgcggc tgccgcggcg
     2101 cgagcgggct gggctgcacc ggcgctgcac tgacgcgcgc cgccgagccg gccagcagcg
     2161 aggccgcggc gatcttgccc agtccaatgc cgcagtcgtg caggaagtgg cggcgggaaa
     2221 cactcagcat ggctattcct tcgtgatcgc ttcgtcgaga ttgagaatcg cc
//
LOCUS       DASRJB010000137         5304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3706152, whole genome shotgun sequence.
ACCESSION   DASRJB010000137 DASRJB010000000
VERSION     DASRJB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706152"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..2269
                     /locus_tag="VFB80_03925"
     CDS             92..2269
                     /locus_tag="VFB80_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN32936.1"
                     /translation="MNSSTDSRLPTPDSLDQEPERYELRQPPRYSFGPDRRDFLKIAG
                     GGIAVCLLTGPARAQQRGKGGGRRGGGFGGGGASQVMGAWLHIGEDGQITLYSGKTEV
                     GQNVRTSVTQALAEELRVKPEAIRVVLADTDLCPNEGGTSGSRSTPATIPQVRRVGAA
                     AREMLIDLAAEQLKAPRGELTAGDAKVTHGASDRSLKYGELTKGQKLTKTVAGDAPLT
                     PTKDWKVLGTSTPKVGGQEIVTGTHQYASDIQLPGMLYGKLLAIPKNSPHGKVGGLMP
                     AGAERVQTSIDATESYLQVLGPSAFEVDLAAAHFRGRLQAPPADQQSSHKTLFADLKR
                     GARVEESPGIEAALKAADHKLEATFTIAYIAHAPLEPRAAVAEWKDGKVTVWTGTQQP
                     SRVKSDLMQAFGLAADKVRVIVPDTGAGYGGKHTVDTAVEAAKLAKAAGKPVKVVWTR
                     EEEFTYAYFRPAGVIDVRAGMTKDGRVTAWDFHNYNSGQSAVRGLYDIPQQRAQFHNA
                     AAPLKQGSYRALASTANTFAREAAMDELAHKVGMDPLAFRMLNLKDERLRAVFQAAAE
                     KFGWGKIKPAENHGLGIAGGSEKGSFVASCAEVVRDPASGKIRVTRVVTAFECGTVLN
                     PDLVTNQIEGAVIQGLGGALFEEIQFEGTKIHNASFSSYRVPRFADTPELETVLVNRP
                     DLAPIGAGETPIIAVAPAIAQAAFQVTGIRAHSLPLAASGIRS"
     gene            complement(2305..3801)
                     /locus_tag="VFB80_03930"
     CDS             complement(2305..3801)
                     /locus_tag="VFB80_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32937.1"
                     /translation="MTLSRRRFVQTAAAASAAFPLFTIAGTKASGKVIGANDTVRIGV
                     AGAGGRGGNHVTEWTSQEKVQITYLIDPDTKNSGSKAVTVEGRQKTRPKTVQDIRQAL
                     EDKELDAVSVATCNHWHSLITIWACQAGKDVYVEKPISHNVFEGRKCVEAAKKYGRVV
                     QHGTQSRSSRSFAKAIAAVHSGKYGELLVSHGSSSKPGSGRSSLGFRPYSTPPSNFDF
                     NLWLGPAPLQPFHANLHPYNWHWFWDTGNGEIGNQGVHQMDIARWGIKSGNLPTKVYS
                     LGGRFLHNGPDQGQAPNMQLAVMEFGQAILLFEVRGLTGKHPDYPADVSNRWVTTEGV
                     IKEGGGRGGRGGGYMFFPKNGGEPQPLVAEEPKITVADAFPAFINAMRTRKPEDNNCD
                     AEVAHYSAACIHVSNASYRLGSGDPKNYEKAEAMVAGHHPEVKAVLERIRDNCKALGM
                     PMDEATWTIGPALTFDPATEKFTGDNAEAANALITREYRKPFVVPEQV"
     gene            complement(3980..>5304)
                     /locus_tag="VFB80_03935"
     CDS             complement(3980..>5304)
                     /locus_tag="VFB80_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN32938.1"
                     /translation="LSIVPMPSEEQLKKYTDQVYRNCPYSDKQLTLAPSDRKTAIPTK
                     VGEASPIKHVIYIIKENRTYDQVFSDIPRGRRDPSLLMFGEEVTPNHHKLANEFVLLD
                     NLYCNGHVSADGHPWSTMAYNTDYIARNWALTYSKRLGIDDDDDGDLSRAPSGYIWDS
                     CKRAGLSYRSYGEYGGRVSQPDGTFRMEGRVPGLVGHVSPKYDLRVEGRPRMRDTDNV
                     AVFLEEFREYEKNGDLPRFIVMSLGEDHTQGTTPGAPTPQACVASNDLALGQLVEAVS
                     KSKYWPQIAIFVIEDDAQNGPDSVDAHRTIGLVISPYVRKGALDSTQYATVSMLRTME
                     LILGLPPLSQFDASANPMFNSFTDQPDLTPYQHVQPVKINLSARNERTAYGAERSQKM
                     DFTEYDKIDDFELNEILWRSIKGADAPIPPSVRRAIAYRGVEVPEAKR"
BASE COUNT          842 a         1705 c         1819 g          938 t
ORIGIN      
        1 tcatcgaggg catgaacggc aacatctgcc gctgcggcac ctacacgcgg atcatcgctg
       61 ccatccagct ggcggcgaag ggaggtgcga aatgaactca tctacggact cccgactccc
      121 gacccccgac tccctcgacc aagagcccga gcgctacgag ctccgccagc cgccgcgcta
      181 ctcctttggc cccgaccggc gcgactttct gaagattgcc ggcggcggca tcgcggtttg
      241 cctgttgacc gggcccgcgc gggcgcagca gcgcggcaag ggcggcgggc ggcgcggcgg
      301 cggatttggt ggcggcggcg cgtcgcaagt gatgggcgcc tggctgcaca ttggcgagga
      361 cggccagatc acgctctaca gcggcaagac cgaggtcggc cagaacgtcc gcacttcggt
      421 gacgcaggcg ctggccgaag agctgcgggt caagcccgag gcgattcgcg tcgtcctggc
      481 ggatacggat ctgtgcccca atgaaggcgg cacttccggc agccgctcga cgccggcgac
      541 gattccgcag gttcgccgcg tcggcgcggc cgcccgcgaa atgctcatcg acctggccgc
      601 ggagcagctc aaggcgccgc gcggcgagct gaccgcgggc gacgccaagg tgacgcacgg
      661 ggcgagcgat cggtcgctga aatacggcga gctgaccaag ggccagaagc tgaccaagac
      721 cgtcgccggc gacgctccgc tcacgccgac gaaggactgg aaggtgctgg gcacctcgac
      781 gccgaaagtc ggcggtcagg agatcgtgac cgggacgcac cagtacgcgt cggacattca
      841 gctgccgggg atgctttatg gcaagctcct cgccattccc aaaaactcgc ctcatggcaa
      901 agtgggcggc ctgatgccgg caggggccga acgagttcag acttccattg acgccaccga
      961 aagttatctg caagtgctcg gaccgtcggc gtttgaggtc gatctggccg cggcacattt
     1021 tcgcggtcgt ctgcaagcgc cgcccgcgga tcagcagtcc tcgcacaaga cgctcttcgc
     1081 cgatctgaaa cgtggggcac gcgtggaaga gtcgccgggt atcgaggccg cgctcaaggc
     1141 cgcggaccac aagctggagg cgaccttcac gatcgcctac atcgcccatg cgccgctgga
     1201 gccgcgggcg gctgtggcgg agtggaagga cggcaaggtg acggtctgga ccggcacgca
     1261 gcagccgtcc cgcgtgaaga gcgacctgat gcaggcgttt ggcctcgcgg cggacaaggt
     1321 gcgggtgatc gtgcccgaca ccggcgcggg atacggcggc aagcacacgg tcgatacggc
     1381 tgtggaggcg gcgaagctgg ccaaggcggc cggcaagccg gtgaaggtcg tgtggacccg
     1441 cgaagaggag ttcacctacg cctacttccg cccggcgggc gtgatcgacg tgcgggccgg
     1501 catgacgaag gacggccgcg tcacggcttg ggacttccac aactacaact cgggccagtc
     1561 ggccgtgcgc ggcctctatg acattccgca gcagcgggcc cagttccaca atgcggccgc
     1621 gccgctcaag cagggctcgt accgcgcgct ggcttcgacg gccaacacgt tcgcccgcga
     1681 agcggcgatg gacgaactgg cccacaaggt cggcatggac ccgctggcct tccgcatgct
     1741 gaatctcaag gacgagcggc tgcgagccgt gtttcaggcg gcggccgaga aattcggctg
     1801 gggcaaaatc aaaccggccg agaatcacgg cctcggcatt gccggcggct ccgagaaagg
     1861 gagcttcgtc gccagctgcg ccgaagtcgt ccgcgacccg gccagcggca agatccgcgt
     1921 gacgcgggtc gtcacggcct tcgagtgtgg gacggtcttg aatcccgatc tcgtcaccaa
     1981 ccagatcgag ggcgcggtca ttcagggcct gggcggggcg ctgttcgagg agatccagtt
     2041 cgaggggacg aaaatccata acgcgtcgtt ttcttcctac cgcgtgccgc ggtttgccga
     2101 cacgccggag ctggagacgg tcctcgtcaa ccggcccgac ctcgcgccga tcggcgcggg
     2161 cgagacaccg atcatcgccg tcgccccggc gatcgctcag gccgcgttcc aggtcaccgg
     2221 catccgcgcc cactcgctgc cgctggccgc gagcgggatc cgatcgtagc ccgccactcc
     2281 gtggcgggct acacggagcg tggcctaaac ctgctccggc acgacgaacg gcttgcggta
     2341 ttcgcgggtg atcagggcgt tggcggcttc ggcgttgtcg cccgtgaact tctccgtggc
     2401 ggggtcgaac gtcagcgccg ggccgatcgt ccaggtcgcc tcgtccatcg gcatgcccag
     2461 cgctttgcag ttgtcgcgga ttcgctccaa aaccgcttta acctccgggt gatggccggc
     2521 gaccatcgct tcggccttct cgtaattctt cggatcgccg ctgcccagcc gatacgacgc
     2581 gttcgacacg tggatgcagg cggccgaata gtgagccacc tcggcgtcgc agttgttgtc
     2641 ttccggcttg cgggtccgca tcgcgttgat gaacgcgggg aacgcgtcgg ccacggtgat
     2701 cttcggctcc tcggccacca gcggctgcgg ctcgccgccg ttcttgggga agaacatgta
     2761 gccgccgccc cggccgccgc gaccgccgcc ttccttgatg acgccctcgg tggtgaccca
     2821 gcggttggaa acgtccgcgg gataatcggg gtgcttgccg gtcaggccgc ggacttcgaa
     2881 cagcaggatc gcctggccga actccatcac cgcgagctgc atgttcggcg cctggccctg
     2941 gtcggggcca ttgtgcagga accggccgcc caggctatag accttcgtcg gcaggttgcc
     3001 gctcttgatg ccccagcggg cgatgtccat ctggtgcacg ccctggttgc caatctcgcc
     3061 gttgcccgtg tcccagaacc agtgccagtt gtacgggtgc aggttggcgt gaaacggctg
     3121 cagcggggct ggcccgagcc agaggttgaa gtcaaagttg ctgggcggcg tcgaataagg
     3181 gcggaagccg aggctggagc gcccgctgcc cggcttgctg gagctgccgt ggctgacaag
     3241 caattcgccg tatttgcccg aatgcaccgc cgcgatcgcc ttggcgaacg accggctgct
     3301 gcggctctgc gtgccgtgct gcacgacgcg gccgtacttc ttcgccgcct cgacgcattt
     3361 gcggccctcg aagacgttgt ggctgatcgg cttctcgacg tacacgtcct tgccggcctg
     3421 gcaggcccag atcgtgatga gcgagtgcca gtggttgcag gtcgcgaccg agaccgcgtc
     3481 gagttctttg tcctccaggg cctggcggat gtcctgtacc gtcttgggcc gcgtcttttg
     3541 ccggccttcg accgtgaccg ccttcgagcc cgaattcttc gtatcggggt cgatcaggta
     3601 cgtgatctgg actttctcct ggctggtcca ttcggtgaca tgattgccgc cgcggccgcc
     3661 ggcgccggcc acgccgatgc ggaccgtgtc gttggccccg atcactttgc ccgaggcctt
     3721 cgtcccggca atcgtaaaca gcggaaaagc ggcgctggcg gcagccgcgg tttgtacgaa
     3781 tcggcggcgg gagagggtca tggcagggct cctgttgcgg acggttggag gacaggcccg
     3841 aattacaaca tggccggtgg gcaaagtcca gcgcgggcgg gagtggtgag tcggtaggtc
     3901 ggtggtcggt aagtcggtgg cacgtgctct gcgtaccgac tgaccgacta accgaccaac
     3961 cgattgaccg gcataccatt caccgcttcg cctccggcac ttcgacgcct cgataggcga
     4021 tcgcccggcg caccgatggc ggaatcggcg cgtccgcgcc tttgatcgag cgccagagga
     4081 tttcgttgag ctcgaagtcg tcgattttgt cgtactcggt gaagtccatc ttctgcgagc
     4141 gctctgcgcc gtacgccgtc cgctcgttgc gggcgctcaa gttgatcttc acgggctgca
     4201 cgtgctgata aggcgtcagg tcgggctggt cggtgaacga gttgaacatc gggttggccg
     4261 aggcgtcgaa ctgcgagagc ggcggcaggc ccaggatcag ctccatcgtc cgcagcatgc
     4321 tgaccgtcgc gtactgcgtg ctgtcgagcg cgcccttgcg gacgtagggg ctgatcacca
     4381 ggccgatcgt gcgatgggca tcgaccgagt cggggccgtt ctgggcatcg tcctcgatca
     4441 cgaagattgc gatctgcggc cagtacttgc tcttgctgac cgcttcgacg agctggccga
     4501 gggccaggtc gttgctggcg acgcaggcct gcggggtcgg tgcgccgggc gtcgtgccct
     4561 gggtatggtc ctcgcccagg ctcatcacga tgaaccgcgg caggtcgccg tttttttcat
     4621 actcgcggaa ttcctccagg aacacggcga cattgtcggt gtcgcgcatc cgcggccggc
     4681 cttcgacgcg caggtcatac ttcggcgata cgtgcccgac gaggcccggc acgcggccct
     4741 ccatccggaa ggtcccgtcg ggctggctca cgcggccgcc gtattcgccg tagctgcggt
     4801 acgacaggcc ggcccgcttg cacgaatccc agatgtagcc gctaggggcc cgcgacaggt
     4861 cgccgtcgtc gtcgtcatcg atccccagtc gcttcgaata ggtcagggcc cagttgcggg
     4921 cgatgtagtc ggtgttgtag gccatcgtgg accagggatg cccgtcggcc gagacgtgcc
     4981 cattgcagta caggttgtcg agcagcacga actcattcgc cagcttgtgg tggttcggcg
     5041 tgacctcctc gccgaacatc agcaggctcg ggtcgcggcg gccgcgcggg atgtcgctga
     5101 aaacctggtc gtaggtgcgg ttctccttga tgatgtagat cacgtgcttg atcggcgagg
     5161 cctcgccgac cttcgtggga atcgccgtct tgcggtcgct cggcgcgagc gtgagctgct
     5221 tgtcggagta cgggcagttg cgatagacct ggtcggtgta cttcttcaat tgctcttcgc
     5281 tgggcatcgg cacgatcgac agcg
//
LOCUS       DASRJB010000138         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2296015, whole genome shotgun sequence.
ACCESSION   DASRJB010000138 DASRJB010000000
VERSION     DASRJB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296015"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..891
                     /locus_tag="VFB80_03940"
     CDS             70..891
                     /locus_tag="VFB80_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN32939.1"
                     /translation="MKLGLINSAWVQANQPTVYGLRKTKELGFDTVDIFVDPLDIDVR
                     ERRLIKDECDRLELPIVSIACVAVGLIDFNPSVQRFHLDRVKRYLDLAFEYEARNVLL
                     VLGEYIWNQEVIPPAEQWKTGVENCRRLGDYASQLGVQIALELEPFHLSLVNSVDSMV
                     RFLDDCNHPAVRANIDVSHLLLAKVAPEELRRLKGKAIHVHISDCDGQKHGDLPPGRG
                     VVPFEPYLREIKALGIDGAISIELEYSPQPDKITEWVAEAYQATDRLMRQAGLRG"
     gene            complement(909..1874)
                     /locus_tag="VFB80_03945"
     CDS             complement(909..1874)
                     /locus_tag="VFB80_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN32940.1"
                     /translation="MRLTTYILAVLLAAASWSIGTGQPPPEPALQAEVARLIRQLDAD
                     RYEQREQASQALRQIGTPALAALRQARQHASMEVRWRARAIVDELTVGVRRRELVAFC
                     SQSDERLDLEQGMWLIARIIDPEVQRSEITRQLDALAARVRERLGKGVDPAAADPQAA
                     VAALRDVLFVQEGFTGNADDYSNPDNNAVHRVLTTHKGLPILLSHVTIAVARRLRIPI
                     AGVPVSPYIVKYDGARAPAGYAKDDIYFHPYEQGRLLSREDRVKDFAGEDPDRMEPEG
                     SRREILLRMLRNLISAYSNRGQGEKLEEAEELKSLLEAFAKPAEP"
     gene            complement(1982..>2727)
                     /locus_tag="VFB80_03950"
     CDS             complement(1982..>2727)
                     /locus_tag="VFB80_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32941.1"
                     /translation="KAAKMAATAKRLGFPLMAGSSLPVTWRVPQVELPRASPLVEGVC
                     TFGYDRGLAEIYLFHGLEVLQCMLERRRGGETGAKTVTCLEGDAVWKACDEGRISWPL
                     VEESIGRCPSANVGPIRENVLKPLAILIEYADGTRGAALNLIEAVSEFGFAGRLKDQQ
                     EPVSCLFHLPEPPGARFFDPLTWHIEQHFYRRPTYPVERTLMTSTVLDLALHSLKDGS
                     RPISSEALDIHYQPPASSGFFRGPVMDRH"
BASE COUNT          439 a          937 c          894 g          457 t
ORIGIN      
        1 gtcagttggc gtgcttcacg actcaccact caccactcac cactcaccca ctaactacta
       61 accacaccca tgaaactcgg actcatcaac tccgcctggg tgcaagccaa tcagccgacc
      121 gtctatggcc tccgcaagac caaggagctg ggcttcgaca cggtggacat cttcgttgat
      181 ccgctcgaca tcgacgtccg ggagcggcgg ctgatcaagg acgagtgcga ccgcctggag
      241 ctgccgatcg tctcgatcgc ctgcgtggcc gtcggcttga tcgatttcaa ccccagcgtg
      301 cagcggtttc atctcgatcg ggtgaagcga tacctcgacc tggctttcga gtacgaggcc
      361 cgcaacgtgc tgctcgtcct cggcgagtac atctggaacc aggaagtgat tccgccggcc
      421 gagcagtgga agacgggcgt ggagaattgc cgccggctgg gcgactatgc cagccagctc
      481 ggcgtgcaga tcgcgctgga gctggagccg ttccacctgt cgctggtcaa cagcgtcgat
      541 tcgatggtcc gcttcctcga cgactgcaat catccggcgg tgcgggccaa catcgacgtc
      601 tcgcatctgc tcctggccaa ggtcgcgccg gaggagctgc gccgcttgaa gggcaaggcg
      661 atccacgtac acatttccga ctgcgacggc cagaagcacg gcgatctccc gcccggccgc
      721 ggcgtcgtgc ccttcgagcc ctacctccgc gagatcaagg ccctcggcat cgacggcgcg
      781 atctcgatcg agctcgaata ctcgccccag cccgacaaga tcaccgagtg ggttgccgag
      841 gcgtatcaag ccaccgaccg gctaatgcgg caagcaggcc tgcgcggcta gctgtcgctg
      901 gtggccgtct acggctctgc gggcttcgcg aacgcttcca gcagcgactt cagctcttcc
      961 gcctcttcca gcttctcgcc ctggccgcgg ttcgagtagg ccgagatcag gttgcggagc
     1021 atgcggagca ggatttcgcg gcgcgagcct tccggctcca tccggtcggg atcttcgccg
     1081 gcgaagtcct tgacgcggtc ctcgcggctg agcaggcggc cctgctcgta gggatggaaa
     1141 tagatgtcgt ccttggcata tcccgcgggc gcgcgggcgc cgtcgtactt cacgatgtac
     1201 gggctcacgg gcacgccggc gatcggaatc cgcagccgcc gggcgacggc gatcgtgacg
     1261 tgcgagagca ggatcggcag gcccttgtgc gtggtcagca cgcggtgcac ggcgttgttg
     1321 tcggggttgg aatagtcgtc cgcattgccg gtgaagcctt cctggacgaa gagcacgtcc
     1381 cgcagcgcgg cgaccgcggc ctgcggatcg gcggccgcgg gatcgacgcc cttgcccaga
     1441 cgctcgcgga cgcgggccgc cagcgcatcc agctgccggg tgatctccga ccgctgcact
     1501 tccggatcga tgatccgcgc gatcagccac atcccctgct ccaggtccag ccgctcgtcg
     1561 ctctgcgagc agaacgccac cagctcgcgc cgccgcactc ccacggtcag ctcgtccacg
     1621 atcgcgcggg cccgccagcg aacctccatg ctcgcgtgct gccgcgcctg gcgcagcgcc
     1681 gccagcgccg gcgtgccgat ctggcggagg gcctgcgagg cctgttcgcg ctgctcgtag
     1741 cggtcggcgt ccagctgccg gatcagccgg gcgacttcgg cctgcaaggc cggttccggg
     1801 ggcggttgac cggtgccgat ggaccacgaa gccgccgcca agaggaccgc gaggatatac
     1861 gtcgttagtc gcataaggag ctgcgcgtgc acccattcgg agcatagcct tccagccccg
     1921 cgcgggccag cctgacggca attcgtgtag tcctctcgcc gagcgagcgg actacacttg
     1981 cctaatgccg atccatgacg ggtccgcgga aaaagccgct gctggccggc ggctgatagt
     2041 ggatatccag cgcctcgctg gaaatcggcc ggctgccgtc cttgagcgag tgcagggcca
     2101 ggtcgagcac ggtgctggtc atcagggtgc gttcgaccgg gtacgtcggc cgcctataga
     2161 agtgctgctc gatgtgccag gtgagcgggt cgaagaatct cgccccgggc ggctcgggca
     2221 gatggaacag gcagctcacc ggctcctgct ggtccttcag gcggcccgcg aagccgaact
     2281 ccgagacggc ctcgatcagg ttgagggccg ccccccgcgt gccgtcggcg tattcgatca
     2341 ggatggcgag cggcttcagg acgttctcgc ggatcgggcc aacgttggcg ctggggcagc
     2401 ggccgatcga ctcctcgacg agcggccagc tgatgcggcc ctcgtcgcag gccttccaga
     2461 cggcatcccc ctctaggcag gtcaccgtct tggctccggt ctccccgccc cgccgccgct
     2521 ccagcatgca ctggagcact tccaggccgt ggaacaggta gatctcggcc aggccgcggt
     2581 cgtagccaaa cgtgcagacg ccctcgacca gcggcgatgc gcggggcagc tcaacctgcg
     2641 gcactcgcca ggtcaccggc agcgacgacc cggccatcaa cggaaagccg aggcgtttgg
     2701 ccgtggcggc catcttggcg gccttct
//
LOCUS       DASRJB010000139         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1732121, whole genome shotgun sequence.
ACCESSION   DASRJB010000139 DASRJB010000000
VERSION     DASRJB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732121"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..564
                     /gene="gspG"
                     /locus_tag="VFB80_03955"
     CDS             115..564
                     /gene="gspG"
                     /locus_tag="VFB80_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HZN32942.1"
                     /translation="MTAARNRPRADDYRGFTLPELVFVLSFIGVAIVVFVSASSFVKR
                     SECTPHNSVKSVVSILEEATNMYVLNIGSCPTAKQGLDALVVPPADLADPTKWMGPYL
                     QKPAVPLDAWNNPYHYQALGGGRFRIWSSGPNGKNEQCRGDDIATSL"
     gene            575..1183
                     /locus_tag="VFB80_03960"
     CDS             575..1183
                     /locus_tag="VFB80_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN32943.1"
                     /translation="MRRRDGYTLMEMLLVLAILAAIAAISVPALQGVMKRAQLTSAGD
                     TVRIELTKAHVKAMKTGRIQVFTFEVNGRKYRTQPWIGDDDELERAGDVQDGGSGKGL
                     STAPEDTAPEKELPEGTMFAAGEAAEESRGMAASEAAQAGGLSDWSRPILFYPDGSSS
                     DAYVVVADEFERGMRIDLIGLTGTAKVGQVTALESLTTGTGP"
     gene            1180..2058
                     /locus_tag="VFB80_03965"
     CDS             1180..2058
                     /locus_tag="VFB80_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN32944.1"
                     /translation="MTRRGLSLLEVILAIAILGMALATIGQLIRIGARNAVMARDLST
                     AQLYCESTLNEVACGAIEPTAGSGQLDDLGDWTYELTVEPTDQENMLSVKVVVTQDAE
                     QYSRPVSFTMTRWMIDPVFVEEAAAKEAEMKALFAQAQQAATSTQSTATGGLASDLSA
                     AAAAGVDASIVGGMIGGGAPAGQGGQGSQGGARGRGGKGGDQAGGGRGQGGRGFGGRG
                     GGQQGGGREQGGGQSRGQGGGQGGRGGGPGGQGGGPFGGGQGGGGFGQGGQGGGGQGG
                     GGFGQGGFGQGGGRGP"
     gene            2048..>2687
                     /locus_tag="VFB80_03970"
     CDS             2048..>2687
                     /locus_tag="VFB80_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32945.1"
                     /translation="MDLSQFCTRTTAFRRERRRGLTLLELILALSLTVLVLMTIGMAI
                     DMHFRMLDVRRTNVEEAVEIRSILNIISKDVGGVMLYVPPDTSGLDAVTGNSATAAAN
                     LAEVAAADPNAAAAAISGLAASAGGSGGGQPAGGTSQVASQTITGGGSISTASTTTGG
                     SGGGPGGGPGGGGGAGGVVAAASGAAAPASSGSSTDPAAATEATAAAPASMVG"
BASE COUNT          476 a          852 c          954 g          405 t
ORIGIN      
        1 ggaaccacga ctaccagtac gaggagctcg acagcggcca gttccgcatc tggtcgagcg
       61 gccccaatgg agtctcgggc gacaacgacg atattcaaac gacgctctag ccagatgacc
      121 gccgcgcgaa accgacctcg agcagacgac taccgcggct tcacgctgcc ggagctggtg
      181 ttcgtgttgt cgttcattgg cgtggcgatc gtggtgtttg tgtcggcgtc tagctttgtg
      241 aagaggagcg aatgcacccc tcacaattcg gtcaaaagcg tggtctccat tttggaagag
      301 gccaccaaca tgtacgtcct caacatcggg tcttgcccaa cagccaagca gggcttggat
      361 gcgttggtag tgccaccggc ggacctggct gatcctacca agtggatggg gccatatctg
      421 caaaagcctg cagttccact tgatgcctgg aacaacccgt accactatca agccctcggc
      481 ggaggcaggt tccgcatatg gtccagcggc ccgaacggta agaatgagca gtgtcgtggc
      541 gatgacatcg ccacctcgct gtgaggaggc cgccgtgcga cgccgtgacg gctatacgct
      601 gatggaaatg ctgctggtgc tcgccatcct ggcggccatc gcagccatct ccgtgccggc
      661 cctgcaaggc gtgatgaagc gggcccagct caccagcgcc ggagatacgg tgcggatcga
      721 gctgaccaag gcccacgtca aggcgatgaa gacggggcgg attcaggtct tcaccttcga
      781 ggtgaacggc cgcaagtatc gcacgcagcc ctggatcggc gacgatgacg agctcgagcg
      841 tgccggcgac gtgcaggacg gcggcagcgg aaagggcctt tcgactgctc ccgaggacac
      901 tgccccggag aaggagttgc ccgaagggac gatgttcgcg gccggcgagg ccgccgagga
      961 aagccgcggc atggcggcca gcgaggcggc tcaggcaggc ggcctcagcg actggtcgcg
     1021 gccgatcctg ttctatcccg acggctccag ctccgacgcc tacgtcgttg tcgccgacga
     1081 gtttgagcgc ggcatgcgga tcgacctcat cgggcttacg ggcactgcca aggtcggaca
     1141 ggtcacggct ctggaatcgc tgaccacagg aactggccca tgacacgccg cggtctatcg
     1201 ctgctggagg tcatcctggc gatcgccatc ctgggcatgg ccctggcgac aatcggccag
     1261 ctgatccgca tcggcgcgcg gaacgcggtc atggcccgcg acctgtccac agcgcagctt
     1321 tactgcgaga gcacgctcaa cgaagtcgcc tgcggggcga tcgaaccgac ggccggctcc
     1381 ggccaactcg acgacctggg ggattggact tacgagttga ccgtggagcc gactgaccag
     1441 gagaacatgc tctcggtgaa ggtcgtggtc acgcaggacg ccgagcaata ctcccggccg
     1501 gtctcgttca cgatgacccg ctggatgatc gacccggtgt tcgtcgaaga ggccgcggcg
     1561 aaggaagccg agatgaaggc gctcttcgct caggcccagc aggcagccac ctccacgcaa
     1621 tcgacggcta ccggcggcct ggccagcgat ctgtccgccg ccgctgctgc cggagtggat
     1681 gccagcatcg tcggcggaat gatcggcggc ggcgccccgg caggtcaggg cggtcaaggc
     1741 agccagggcg gcgctcgcgg gcgcggcggc aaaggcggcg accaggccgg cggcggacgc
     1801 ggccagggcg gtcgcggttt tggcgggcgc ggcggaggcc aacagggcgg tggtcgcgag
     1861 caagggggtg gtcaaagtcg cggtcaaggg ggtggtcaag gcggtcgtgg tggcggcccc
     1921 ggcggtcaag gcggcggccc attcggcggc ggacagggcg gcggtggatt cggccaaggt
     1981 ggccagggcg gcggcggaca gggcggcggc ggcttcggtc aaggcggctt tggccaggga
     2041 ggcggccgtg gaccttagtc aattctgcac ccgcaccact gccttccggc gagagcgccg
     2101 gcgcgggctc acgctcctgg agctgatcct tgcgctctcg ctgacggtgc tggtgctgat
     2161 gacgatcggc atggcaatcg acatgcactt ccgcatgctc gacgtccgcc gcaccaacgt
     2221 cgaagaggcg gtggaaatcc gctcgatcct caacatcatc tccaaagacg tgggcggcgt
     2281 gatgctctat gttccgcccg acaccagcgg tttggacgcc gtgaccggca actccgcgac
     2341 tgcagccgcg aacctggccg aagtggcagc tgccgacccc aatgcagccg cggccgcgat
     2401 ctctgggctg gcggccagcg cgggcggatc cggcggcgga cagcctgcgg gcggaacgtc
     2461 gcaggtcgcc agccagacga ttaccggcgg cggatcgatc tcgactgctt cgaccacgac
     2521 gggcggctca ggaggcggcc caggaggcgg tcctggtggc ggcggaggcg caggcggtgt
     2581 agttgccgct gccagcggcg cggccgcacc agcctcgagc ggcagcagca ccgaccccgc
     2641 tgcggcaacc gaagcgacgg cggcagcccc agccagcatg gtcggtc
//
LOCUS       DASRJB010000140        12936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2578483, whole genome shotgun sequence.
ACCESSION   DASRJB010000140 DASRJB010000000
VERSION     DASRJB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578483"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFB80_03975"
     CDS             <1..315
                     /locus_tag="VFB80_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN32946.1"
                     /translation="VIVQVHKRQGDWVEPGDKMLRLIRLDRLRVEGFLDAAQVKASLE
                     GRPVALTLDPPASPGVAFSGKLIFVSPEIDPINRQVRVLAEIENPNFALQPGSRGTLT
                     IR"
     gene            321..2531
                     /locus_tag="VFB80_03980"
     CDS             321..2531
                     /locus_tag="VFB80_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HZN32947.1"
                     /translation="MLASATVDRPLLLRQRRDLVVREQWFAGQRHFVLHDPLALTYAY
                     LTEIEHAVWALLDGRTSSAEVLAQFAARYAPRQLTQHELQSFLGQLHRQGLVVSDAPG
                     QGTQLAERHERKERFNLLRLAEKVLALRWRGINPQRLLDWLEPKLGWLFSGWAVALWT
                     AIVAWAAVLAATQGSELARRLPDARAWLSGGNLLWLSAALILVKTLHELGHALAARRV
                     GCRCREIGVQLFFLLPCLYTNVSDVWLVPSKWRRMAVSAAGIYVELLLAAIAMLIWWQ
                     AEPGVLSSLCLNVMLVASVGTLVLNGNPLLRYDGYYLLSDLVEVPNLEQTSRQQLQAL
                     LTRWCLGADLPRVDQFSWSKRLGLAVYAAAALAYRAVLLVGIYFALRSLLAPYRLQPL
                     GDVLLALAVMGMVVPLAIGAGQSIGQARRRRELSPLRLGLTAAILLGLLLAACYIPLP
                     QRLAAPAVIEPQNAVYVYVTVPGTLVSALTPGAAVERGQTIAQLASPELERDHVRLVS
                     EAERQRLHIAALEAGRGDDPAALAALPAARQSLADLQQRLTQVRELIGRLRLTAPQDG
                     IVLPPPQQRHAAKSQQLDRWLGTPLEPANRGSFLESGTLACIVAQPGQVEALAVVQQG
                     DVPLVQSGSSVSIAIPQHPQGALRGVIESVSQIDAAELPIHLTAAGLIPQKLDAQGRP
                     QALDTVYQVRIALDRPPPELRPGATCQVLLTAAPQTLAARLSRWLGQTFRFRRA"
     gene            complement(2559..3425)
                     /locus_tag="VFB80_03985"
     CDS             complement(2559..3425)
                     /locus_tag="VFB80_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32948.1"
                     /translation="MLRTTRLPVGASRLCLALALAALSASGCITPAKIASVEIDTTLP
                     APAANPLKPILVATAPQVADPAQEQTILAPADALPPPTIVTAQPLENAPAPIGPLVPG
                     QAREALPPAPPPAAPPRRADAAALAQIKPIRQISLDITLPVDADPMLNRLAPPTNYAA
                     EALPQMAAQNPFYRGDLIDYGMYDNTPLPPVGLDMCYQPLYFQELNAERYGRSWGCLQ
                     PAVSVVQFYGRIPMLPYMAFAWPARRCTYHAHWALPGYRIPGKEPYPIVISPAGGAAQ
                     AAAMLGVILLIP"
     gene            3894..>12936
                     /locus_tag="VFB80_03990"
     CDS             3894..>12936
                     /locus_tag="VFB80_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32949.1"
                     /translation="MLSWMTGWGRALMKGARRKPGKNRRDHSRDLYPTLQVRMLEERR
                     VFHAGAIVAPTETATPEPPPPTTTVTLDAGQNLLVQDSSADGQNDQLSVRLDAANGRY
                     EIYDPGQLLQTDIAGAEGNGTNRLYIPVEVVTGDKLIFQTGAGDDSLAVDLSGGLGGK
                     SLVFDGGAGENDSLTISGGSYSAVGYVFMGDGSTQIQAGNSALQLTGVEPIFDYLAAD
                     TRQFTIGAGVSGLALGDDGIAANGLSRLETSLGTSVTFASPWHSLTLGAEDSAGQPSL
                     AVSGLDNNFAADLTIDAGDSGRVELSGQIHLHGSDLRASGDTIQVSGSIITGEATIDL
                     SARRQLTVTAEGVLENSAGQVLLDAGGEGTLQFSGRIDASGLAAGQTGGTVHLLGWQV
                     EVLGTASIDASGMSGGGTVLVGGDYQGANPLIRNATNTLVQSETSISANALENGNGGR
                     IIVWADEAAVVAGSGNLQARGGASGGDGGFIETSGKQYLEVRDAADASSPFGNAGTWL
                     LDPTNLTVVAGTGGSLPGGSFTSSPGNSTIGATTITNALQGGTNVTLNTGASGSEAGN
                     ITVNAPISVAVGNGNTRTLTLNAHAVIDVNSAITATSGSLNVVLNAGGAITLDANITT
                     RGGNLTTQGTTFENVGATIDTRGPGALVGGNITLTHTGLVQIGANMLLDEGTANITGV
                     GIQFVGASGLISGRVATLNAGTSDITSGGATSDITVSGAGAAVSLQGTAIGTSSNRLV
                     VNLGAAGTLGLNATSGDIYADLAGGSFSTSRITTLSAGGTGADIDLQVTSGNLTIDNV
                     AFADSVADNTANDDFVLTSTSGNVVLSNSSALVAATVKLSANGTLTATTSGPHLNTSG
                     TGTAITLMAGGTIGTSANRIGVQAGTGLVTATTTSNTATDGVFLASTGRLSLGAIATG
                     TAIQSVNLTTSGTTPDVDISAVNSGNDNWTINSAGAIAFSGLGSLAANSLALTAASAI
                     NLGTASLTSTGAANLTATAGNISFGAAGSITAASATLLAGGDILGSSANATDINTSGS
                     NGNITLSADSLGASGQPLQLNPGDGILNLTATTGGVFVQLQAGDLLFSKLSLNVQAAN
                     QQIVLSTLNGDIRLDNLGGLVMTADDDWTLTTGGNNRDIAFTASAALFSDALTLSATR
                     NIGSSVNPFLMQPSGPISASAGGNVFLRKATGNLDTGDFTLLESTQSGGIVSIAAANG
                     SVNVDDVAGLSVANDTLRINASGGIAFSTTLTANRVELTAGGNIASATGPGVDIAAVG
                     GISLVAGGIGASGERLKVNPGSAAISAATSGSGAAGSIYLESSAALDLAAFTTDTGTA
                     QTVDLLTTAGDLTVSAITSNDNWILSATGDIVFQGTLTAASVGLNAGGNITSAPGNGV
                     DISAAGGIALVAGGGIGASGQRLIVNPGSSALSASTSGANAAGSIYLQSSSALDLNAF
                     TTAAGSVQTVDLRTTAGDLTISTAINSNDNWILSATGDIVFQATLTAASVSLDADGNI
                     TSATGAGVDISAAGGISLTAGGGIGASGERLKVNPGSGAISANTSGDAADGSIYLESS
                     AALNLNAFTTDAASTQTIDLLTTAGDLTISTTINSNDDWFLNSTGNITFGAATLTARH
                     VTLDAGGNITSGTAAVDVIATGNAVTGGVTLIAGGGIGASGERLIVNPGSGALSATTS
                     GVGLDGSIFLESSSGLDLSAFTTVDGSTQTVDLRTTGGNLTISAAIDTDDDWFLDATG
                     NIIFDATGSLKVNSANLTAGGTITSLGSGTDIDTSGVGGDITLSADSLGASGQPLQLN
                     PGAGDLDLTATTGGVFVQLQAGSLLFSRMSLDVQAANQQIVLSTLDGHITLDNLGGLV
                     NTANDDWTLIAGGTDRDITFTSATALTSDALTLSATRNIGSSVNPFLMQPSGAIGAIS
                     ATAGGDIFLRKASGDLLTSNFAALTSTKAGGGFVSIGTKDGAVNVNSIAAGVDLDDDT
                     LEIVTGGDAGDTHAITLNTTLAAGAVRLTALGAIIGNAGDTTDIVTTSATGITLSGTS
                     IGASGTPLEVNSGAGQLDVTAKTGGVFIEFDGATLQTSLVSLDVAGSGQTIFLSTGSG
                     SIAVDGVDGDPLDGTDGFDVNTRDDHFTLRTGGSNQAIAFSGATTLTGATVTLDSSGA
                     ITSGAATDVVSLAGDIILLANGNIGAAGNELLLQSAANMQVSGDANIHVRQATGNLLF
                     GRFTVLTSSADDATVSVGTVSGNVSVDLIQPGVAVGANRLEIAAGGAGNITFGTTLTA
                     RHVTLDAGGNITSGPAAVDVVATGTALTGGVTLTAGGGIGASGDPFTVQAGAGQVTAT
                     TLGGVPAGNIYLTSSGPLRLGAIATAGGTHDIRLEIVGGGLTIDAASVTTDNWMITAG
                     GNVSFVGSGRIEAHEVRITAAGTVTGSPLDVADIDTSSASGLILLSGTSLGGPLQPLQ
                     LDSGGGEIDLRASAGDIFAEIENAGGFQTSKLKLLVSETGRLISLDVSGELVFDDVGG
                     FTSTGDDNFVVRTVDGPYDIHLNNAVPFTAANIELDSSGSIVNHDAAVSSDAPVLTPT
                     GLLRLTAAEDIGAGGQPLVVNPGGQLIVSAGNDIYLRVPGSLATSKFLALTSTAVDGF
                     VSLSSGAVTVDDASGFDLDNDTLEIISTVPVTGDITINSSLSAGSVLLQAAGSIVSST
                     GGPDVTTTDGGTGGDIVLFASRGIGASGNAIVLNPSAAGDITAETAGINTPGDPTAGD
                     IYLSSSSSFRLSGIVTDLSSTQIVSLETTGGSFTIAEASNTNDHWILKSSAAIAFSGD
                     GTIRAHEVELDATGAITSTSTAPVDIDTSAGGPTGTGGDIKLSGTALGASGDSLVLTP
                     GLGNLEATATTGGIFLEFPQNLSLSQFTKLDLQGTSGTLDLSVTSGDLVLDKSVGDST
                     KSQTYSLSAAGNITFEDEAANDPPTLTANIINLTAGADILNESTQPDLPPPENPFQSY
                     QPTANLQATTSITLVADNLGQASDPLQVAPGTGNLTLVASGDTWVNVVGGGIDATKLS
                     VQGSQTVGLSTS"
BASE COUNT         2246 a         4373 c         4275 g         2042 t
ORIGIN      
        1 gtcatcgtgc aggtccacaa gcgccaggga gactgggtcg agcccggcga caaaatgctg
       61 cggctgattc gcctcgaccg tttgcgggtg gaaggattcc tcgacgccgc ccaggtcaag
      121 gccagcctcg aaggccggcc cgtcgctctg acgctcgacc ctccggctag ccccggcgtg
      181 gcgttcagcg gcaaattaat cttcgtcagc cccgagatcg atccgatcaa ccgccaggtc
      241 cgcgtgctgg cggaaatcga gaacccgaac ttcgcgctcc agccgggctc gcgcgggacg
      301 ctgacgatcc ggtagccaac atgctcgcca gcgcgactgt cgatcgtcca ctgctgcttc
      361 ggcagcgccg cgacctggtg gtgcgcgagc agtggttcgc cggccagcga cactttgtcc
      421 tccacgatcc gctggcgctg acgtacgcgt acctgaccga gatcgagcac gcggtgtggg
      481 cgctgctcga cgggaggacg agctcggccg aagtcctggc gcaattcgcg gcccggtatg
      541 ctccgcggca gctcacgcaa catgagctgc agtcgttcct cgggcagctg caccgccagg
      601 ggctcgtggt cagcgacgcg cctgggcaag gcactcagct ggccgagcgg cacgagcgca
      661 aggagcggtt caacctgctg cggctggcgg aaaaagtgct cgcacttcgc tggcgcggaa
      721 tcaaccccca gcggctcctc gactggctcg agcccaaact gggctggctc ttttcaggct
      781 gggcagtggc gctgtggacg gcgatcgtcg cctgggcggc ggtgctcgcc gccacgcagg
      841 ggagcgagct ggcgcggcgg ctgccggatg ctcgcgcctg gctgtccggc ggcaacctgc
      901 tctggctctc cgccgcgctg atcctggtca aaacgctgca cgagctgggg cacgccctcg
      961 ccgcacgccg cgtcggctgc cgctgccgcg agatcggcgt gcagctgttc ttcctgctcc
     1021 cgtgcctgta cacgaacgtt tcggacgtgt ggctcgtgcc cagcaaatgg cggcggatgg
     1081 ccgtgtcggc ggcgggcatc tatgtcgagc tgctgctggc agccatcgcg atgctgatct
     1141 ggtggcaggc ggagccggga gtgctctcga gcctgtgttt gaacgtgatg ctcgtggcct
     1201 ccgtgggcac gctggtgctc aacggcaatc cgctgctgcg ctacgacggc tactacctgc
     1261 tgtccgacct ggtggaggta ccgaatttgg agcagacctc gcggcagcaa ttgcaggcgc
     1321 tgcttacccg gtggtgcctc ggggctgact tgccacgcgt ggatcaattc tcgtggagca
     1381 agcggctggg cctcgccgtt tatgccgccg cggctttggc ttatcgggcg gtgctcctgg
     1441 tcggcattta tttcgcgctc cgctcgctgc tcgcgcccta ccggttgcag ccgctaggcg
     1501 acgtactgct cgccctggcc gtcatgggga tggtagttcc gctggcgatc ggcgcgggac
     1561 aatcgatcgg acaagctcgc cgacgtcgcg agctatcccc cctccggctt gggctgacag
     1621 cggcgatcct cttgggcctg ctcctggcgg cctgttacat tccgctgccg cagcgactgg
     1681 ccgcgcccgc ggtcatcgag ccgcagaacg cggtctacgt ttatgtcacg gtccccggaa
     1741 cgctggtcag tgcgctgacg ccgggagcgg cggtcgagcg cggccagacc atcgcccagc
     1801 tggccagccc cgaattggaa cgcgaccacg tgcggctggt aagcgaggcg gagcggcagc
     1861 ggctgcacat cgccgcgctc gaagcggggc ggggcgacga tcccgccgcg ctggcagccc
     1921 tgcccgccgc tcggcagtcg ctggccgatt tgcagcagcg attgacgcaa gtccgcgagc
     1981 tgatcggccg cctgcggctg accgcgccgc aggacggcat cgtgctgcct ccgccccagc
     2041 agcgccacgc ggccaaatcg cagcagcttg accggtggtt gggaactccg ctggagccgg
     2101 ccaatcgcgg cagctttctg gagtcgggga cgttggcctg catcgtcgcg cagccaggcc
     2161 aggtggaggc gctagccgtc gttcagcagg gggacgtgcc gctggtgcag tcgggcagta
     2221 gcgtcagcat tgccatcccg cagcatcccc aaggcgcgct tcgcggcgtc attgaatccg
     2281 tgtcgcagat cgacgcggcc gagttgccga tccatttgac cgccgcgggc ctgatcccgc
     2341 agaagctcga cgctcagggc cggccgcagg cgctggacac tgtttaccag gtgcgaatcg
     2401 cgctcgatcg cccgccgccg gagctgcggc ccggcgctac atgccaggtg ctgctgacgg
     2461 ccgcccccca aacgctcgcc gcgcggctct cgcgctggct ggggcagaca ttccgttttc
     2521 gacgggcgtg aggccgtgga cttgaagccc ctagcgcgct acggaatcaa caggatcact
     2581 ccgagcatcg ccgctgcctg ggccgcgcca cccgccgggc tgatgacaat cgggtacggc
     2641 tctttccccg gaatgcgata gccgggcagt gcccagtgag cgtgatacgt gcagcgccgc
     2701 gccggccagg caaaggccat gtacggcagc atcggaatcc ggccgtagaa ctgcacgacc
     2761 gagaccgccg gctgcaagca gccccacgag cggccatacc gctcagcatt cagctcctgg
     2821 aaatagagcg gctgataaca catgtcgagc ccgaccggcg gcagcggcgt gttgtcgtac
     2881 attccgtagt cgatcaggtc gccccggtag aacgggttct gcgccgccat ttgcggcaag
     2941 gcttccgccg cgtagttcgt gggcggagcg agacggttga gcatcgggtc ggcatcgaca
     3001 ggcagcgtga tatcgagcga gatctgccgg atcggcttga tttgggccag cgcggcggcg
     3061 tccgcacggc gaggcggtgc agccggcgga ggcgccgggg gcagggcctc gcgcgcctgg
     3121 ccgggcacca gcggtccaat cggagccggc gcgttctcca gcggctgggc ggtcacgatc
     3181 gtcggcggcg gcagtgcgtc ggccggcgcg agaattgtct gctcctgggc aggatcagcg
     3241 acctgcggcg ctgtagccac caagatcggc ttgagcggat tggcggccgg agccggcagc
     3301 gtcgtgtcga tctcgacact cgcgatcttc gccggcgtga tgcagccgct cgcgctcaga
     3361 gccgccagcg ccaaggccag gcacagccgc gaagcgccaa ccgggagtcg ggtcgttcgc
     3421 aacatgcctg gaattccacg ataaagggct ggcgcggatc agccgcgagt agtgatgttg
     3481 tcggccccgt caattcggca tctcgaaatt tcgcagcagt cgcagtgcat tccgagtcac
     3541 cgttatgcga caaagtaccc gaacaaccgg cacggccggc tgtgcgggcg atgccagacg
     3601 tagccgctgc aaaagcgctg cggggtcgct ctgggccgat ccgatagaga agtcggtgaa
     3661 caggcgaagc tggtcgcggg cctccgtgtg cacggcatct gcgtttattg cggattggag
     3721 cggctgaaca tggcgattcc ccccttccgc gggtgtgggg ttcgggcaaa aaatcgctcc
     3781 cggcatcccc caggccatcg ggctagattc gaaatagcgg tatcatcagg ggagatactt
     3841 cgccggccga ttttcagcgg gtccatcggc tgcgcggacc agaggagcgc agcatgttga
     3901 gttggatgac ggggtggggc cgcgcgctga tgaagggcgc gcgccgcaag ccagggaaga
     3961 accgccgcga tcattcgcgg gatctctacc cgacgctcca agtgcggatg ctggaggagc
     4021 ggcgcgtctt tcacgcgggg gcgatcgttg ccccgaccga gacggcgacg ccagagccgc
     4081 ctccgccgac cacgacggtc acgctcgacg ccggccagaa cctgctcgtt caagactctt
     4141 cagccgacgg ccagaacgat caactatcag tccgcctgga cgcggcgaac gggcggtacg
     4201 aaatctacga tcccggccag ctgctccaga cggacattgc cggcgcagag ggcaatggca
     4261 cgaaccggct ctacattccg gtcgaagtcg tgaccggaga caagctgatc ttccagactg
     4321 gggcgggaga cgattcgctg gcggtcgatc tgtcgggcgg gctgggcgga aaatcgctgg
     4381 tgtttgatgg cggggcgggt gagaacgaca gtctgacgat cagcggcgga tcgtattccg
     4441 ccgtcggcta cgttttcatg ggtgatggct caactcagat tcaggccggc aattcggcgt
     4501 tgcaactgac gggcgtcgag cccatcttcg actacctcgc ggccgatacg cggcagttca
     4561 cgatcggcgc gggagtgagc ggcctggcgc tcggcgacga cggcatcgcg gccaacgggc
     4621 tgtcgcgcct ggagacttcg ctgggaacga gcgtcacgtt tgcgtccccg tggcactcgc
     4681 tcacgctcgg cgccgaggat agcgccggcc agccgtcgct ggcggtaagc ggcctggaca
     4741 acaacttcgc cgccgatctt acgatcgacg cgggcgacag cggccgggtg gagttgagcg
     4801 gccagatcca cctccacggc agcgacttgc gcgcctcggg cgacacgatc caggtcagcg
     4861 gctcgatcat caccggcgag gccacaatcg atctgagcgc ccgtcgccag ctcaccgtca
     4921 cggccgaggg cgtgctggag aattccgccg ggcaagtgct gctcgacgcc ggcggggaag
     4981 gcacgctgca gttctccggg cggatcgacg ccagcggcct ggcagcgggc cagaccggcg
     5041 gcaccgtgca cctcttgggc tggcaggttg aagtgctcgg gaccgcgagt atcgacgcct
     5101 cgggcatgag cggaggcggc accgtgctcg tgggcggaga ctatcagggc gctaacccgc
     5161 tgatccgcaa cgcgacgaac acgctcgtgc agtccgagac gtcgatcagt gccaacgccc
     5221 tcgaaaacgg caacggcgga aggatcatcg tttgggccga cgaggccgcg gtagtcgccg
     5281 gctcgggcaa cttgcaggcc cgcggcggcg caagcggggg cgacggcgga ttcatcgaga
     5341 cttccggcaa gcagtatctc gaagtccggg acgcggccga cgcttccagc cccttcggca
     5401 atgccggcac gtggctgctg gacccgacca acctcaccgt cgtggctggt acgggcgggt
     5461 cgctcccggg aggatccttc acctcctctc ccggcaactc gacgattggg gccacgacga
     5521 tcactaacgc cttgcagggc ggcacgaacg tcacgctcaa caccggcgct agcggttccg
     5581 aggccggcaa tatcacagtc aatgcgccga tttcggtggc ggtgggaaac ggcaataccc
     5641 ggacgctcac gctcaacgcc cacgccgtga tcgacgtgaa ttcggccatc acggccactt
     5701 cgggcagcct gaatgtcgtg ctcaacgccg gcggcgcgat cacgctcgac gccaatatca
     5761 ctacccgcgg gggcaatctg acgactcagg gcacgacgtt cgaaaatgtc ggggcgacga
     5821 tcgacacgcg cggtccgggt gctcttgtcg gcggcaacat cacgctgacg cacaccggcc
     5881 tcgtgcagat cggcgccaac atgctgctcg acgagggcac cgcgaacatt accggcgtcg
     5941 gaatccagtt cgtgggcgct agcggattga tctcggggag agtggccacg ctcaacgccg
     6001 ggacgagcga catcaccagc ggcggcgcca cgagcgacat tacggtcagc ggcgccggcg
     6061 cggccgtttc cctgcagggc accgccatcg gtacgagcag caaccggctc gtcgtcaatc
     6121 tgggtgccgc gggcacgctc ggattgaacg ctaccagcgg cgacatttac gccgatctgg
     6181 ccggcggaag cttctcgacc agccgcatca ccaccctcag cgccggcgga accggggcgg
     6241 acatcgatct gcaggtcacg tcgggcaatc tgaccatcga caacgtggcc tttgccgact
     6301 ctgttgccga caacaccgcc aacgacgatt ttgtgctgac ctcgacctcg ggcaatgtgg
     6361 tgctctcaaa cagttccgcg ctggttgccg cgacagtcaa actctcagct aacggcactc
     6421 tcacggccac aacctccggt ccgcacttga acacctcggg cacgggcacc gcgatcacgc
     6481 tcatggccgg cggcacgatc ggcacgagcg ccaaccgcat cggcgtgcaa gccggcacgg
     6541 gcctggtcac cgcgacgacg acgagcaaca cggcgaccga cggcgttttt ctggcttcca
     6601 ccgggcggct ctcgctgggg gcgatcgcga cgggcacggc gattcaatcg gtgaacctga
     6661 ccaccagcgg gaccacgccg gacgtcgaca tctcagccgt caatagcggg aacgacaact
     6721 ggacgatcaa ctcggccggc gcgatcgcat tcagcggtct cggcagcctg gcggcgaact
     6781 ccctggcgct cacggctgct agcgcgatca atctcggcac ggcgagcctg acctccaccg
     6841 gcgctgccaa tttgaccgcc accgccggca acatcagctt cggcgcggcg gggagcatca
     6901 ctgccgcctc cgcgacgctg ttggccggcg gcgacatcct gggcagcagc gccaacgcca
     6961 ccgacatcaa cacgagcggc tcaaacggca acattacgct ctcggccgac agcctgggag
     7021 cgtccggcca gccgctgcag ctcaatccgg gtgacggcat cctcaacctg acggcgacca
     7081 ccggcggcgt gtttgttcag ctccaggccg gcgacctctt gttcagcaaa ttgtcgctca
     7141 acgtacaggc cgcgaatcag cagatcgtcc tctccaccct gaatggcgac atcaggctcg
     7201 acaacctggg cggcctcgta atgacggcgg acgacgactg gacgctcacc actggcggca
     7261 ataaccgcga catcgctttc accgcctccg cggcgctgtt cagcgacgcg ctcacgctct
     7321 cggccacgcg caatatcggc tccagcgtca accccttctt gatgcagccc agcggcccga
     7381 tcagcgcgtc cgccggcggc aacgtcttcc tgcgaaaggc gacgggcaac ctggacacgg
     7441 gcgattttac gctgctggag tcgacccagt ccggcggcat cgtatcgatc gcggcggcca
     7501 acggctcggt caacgtggac gatgtggcag gattgtcggt agcgaacgac accttgcgga
     7561 tcaacgcttc gggcggcatt gcattttcca ccacgctcac cgcgaaccgc gtggaactca
     7621 ccgccggagg caacattgcc agtgcaaccg gccctggcgt cgatatcgcc gccgtgggcg
     7681 gcatctcact ggtagcaggc ggcatcggcg ccagcggcga gcggctgaag gttaatccag
     7741 gctcggcggc gatttcggcg gcaaccagcg gtagcggcgc ggccggcagc atctatctgg
     7801 agtcgagcgc ggccctcgac ctggccgcgt tcaccacgga caccggtacc gcacagaccg
     7861 tcgatctgct gacgactgcc ggcgatctga cggtctcagc gatcacttcc aacgacaatt
     7921 ggattctaag cgccacgggc gacatcgtgt tccagggcac gctgaccgcg gccagcgtgg
     7981 gactaaacgc cgggggcaac attaccagcg cgcccggcaa tggcgtcgat atcagcgctg
     8041 ccggcgggat cgcgctggtg gccggcggcg gcatcggcgc cagcggacag cggctcatcg
     8101 tcaatccagg ttcatcggcg ctttcggcga gcaccagcgg cgctaacgcg gccggcagca
     8161 tctacctcca gtcgagtagt gccctggacc tgaacgcctt taccacggcc gccggctctg
     8221 tgcagaccgt cgatctgcgg acgaccgccg gcgatctgac gatctccacg gcgatcaatt
     8281 ccaacgacaa ttggattctg agtgccacgg gcgacatcgt gtttcaggcg acgctcaccg
     8341 cggccagcgt cagcctcgac gccgacggca acattaccag cgcgaccggc gccggcgtcg
     8401 atatcagtgc cgcgggcggc atttcgctga cagcaggcgg cggcatcggc gctagtggcg
     8461 agcggctgaa ggtcaatccg ggctcggggg cgatttcggc caacacgagc ggcgacgctg
     8521 cagacggcag catctacctg gagtcgagcg ccgccctgaa tctgaacgcg ttcaccacgg
     8581 acgccgcttc cacgcaaacc atcgatctgc tgacgacggc cggcgatctg acgatctcca
     8641 cgacgattaa ttccaacgac gactggttcc tgaactcgac gggcaacatc acgttcggcg
     8701 cggctacgct aaccgcccgg cacgtgacgc tcgacgcggg cggcaacatc accagcggaa
     8761 cggcggccgt tgatgtgatc gccaccggca acgccgtcac cggcggcgtc accttaatcg
     8821 ccggcggcgg catcggcgcc agcggcgagc ggctgatcgt caatcccggc tcgggggcgc
     8881 tttcggcgac cacgagcggc gtcggcttag acggcagcat tttcctggag tcgagcagtg
     8941 gcctcgacct gagcgcgttc acgacggttg acggttccac gcagaccgtc gatctgcgga
     9001 cgaccggcgg caatctgacg atctccgcag cgatcgatac cgacgacgat tggttcctgg
     9061 acgccacggg caatatcatc ttcgacgcta cggggagcct gaaggtcaat tcggccaacc
     9121 tgacagccgg cggcacgatc accagtttgg gaagcggaac cgacatcgac accagcggcg
     9181 ttggcggcga tatcaccctg tcggccgaca gcctgggagc gtccggtcag ccgctgcagc
     9241 tcaatcccgg cgcgggcgac ctcgacctga cggcgaccac gggcggcgtg ttcgttcagc
     9301 tccaggcggg cagcctcttg ttcagccgca tgtcgctcga cgtgcaggcc gcgaaccagc
     9361 agatcgtcct ctccacgctg gacggccaca tcacgctcga caacctgggc gggctggtga
     9421 ataccgctaa cgacgactgg acgctgatcg ccggcggcac cgaccgcgac atcacgttca
     9481 ccagcgccac ggccctgacc agcgacgcgc tcacgctctc ggcgacgcgc aacatcggca
     9541 gcagcgtcaa tccattcttg atgcagccga gcggcgcgat cggcgccatt agcgcgaccg
     9601 cgggcggcga catcttcctg cggaaggcgt ccggcgacct gctcacgagt aacttcgcgg
     9661 cgctgacttc gactaaggcg ggaggtgggt tcgtctcgat cggcacgaag gacggtgcgg
     9721 tcaacgtaaa ttccatcgcc gccggcgtgg atctggacga cgacacgctg gagatcgtca
     9781 cgggcggcga tgccggcgac acccacgcca tcacgctgaa cacgacgctc gcggcgggcg
     9841 cggtccgcct gacagcgctc ggcgcgatta ttggcaacgc gggcgacacg accgacatcg
     9901 tcaccaccag tgccaccggc atcacgctgt ctggcacgag cattggagcg tccggtacgc
     9961 cgctggaggt caacagcggc gccggccagc tcgacgtgac cgcgaagacg ggcggcgtat
    10021 tcatcgagtt cgatggcgca acgctgcaga ccagcctggt gtcgctcgat gtcgcgggca
    10081 gcggtcagac gatctttctt tcgacgggct cggggtcgat cgcggtcgac ggcgtggacg
    10141 gcgaccctct cgacggaacc gacggattcg acgtcaacac gcgggacgac cacttcaccc
    10201 tgcgaaccgg cggctctaac caggccatcg ccttctccgg ggcgacgacg ctcaccggcg
    10261 ctacggtgac gctggattcc tcgggcgcaa tcaccagcgg cgcggccacc gacgtggtca
    10321 gcctggccgg cgacatcatc ctgctggcca atggcaacat cggcgcggcg ggcaacgagc
    10381 tgctgctgca atcggcggcc aacatgcagg tgagcggcga cgccaacatc cacgtgcggc
    10441 aagcgacggg caatctgctc ttcggccgct tcacagtgct gacttcgtcg gccgatgacg
    10501 ccactgtgtc ggttggcacg gtcagcggca acgtgagcgt cgatctcatc caacccggcg
    10561 tcgccgtggg cgccaaccgg ctggagattg ccgctggggg agcgggcaac atcacgttcg
    10621 gcacgactct gaccgcccgc cacgtgacgc tcgacgcggg cggcaacatc accagcgggc
    10681 cggccgccgt ggacgtggtc gccaccggca ctgcccttac cggcggcgtg accttgacgg
    10741 ccggcggcgg cattggcgcc agcggcgacc cctttacggt ccaggcgggc gcagggcagg
    10801 tcactgcgac gaccttgggc ggagtcccgg caggcaacat ctacctgact tccagcggcc
    10861 cgctgcggct gggcgcgatt gccactgcgg gcggcacgca tgacatccga ctcgaaattg
    10921 tgggcggcgg gctgacgatc gatgccgcca gcgttaccac cgacaactgg atgatcaccg
    10981 ccggcggcaa cgtcagcttc gtcggcagtg gccggatcga ggcccacgag gtgcgcatca
    11041 ccgccgcggg aacggtcacc ggcagcccgc tcgacgtggc cgacatcgac accagcagcg
    11101 ctagcggcct gatcctcctc agcgggacga gtctcggcgg gccgctgcag ccgctgcagc
    11161 tcgactcggg cggcggcgag atcgacctgc gggcctcggc aggcgacatt ttcgccgaaa
    11221 tcgaaaacgc cggaggcttt cagacgagca aactcaagct cctcgtcagc gaaactgggc
    11281 ggttgatctc gctcgacgtc tccggcgagc tggtgttcga cgacgtcgga ggttttacct
    11341 cgacgggcga cgacaacttt gtcgtgcgga ccgtggacgg gccttacgac attcacctca
    11401 acaatgccgt gccgttcacg gccgccaaca tcgagctgga ctccagcggc agcatcgtca
    11461 accatgacgc cgccgtcagc tccgatgcgc cggtcctgac gccgaccggc ctcctcaggc
    11521 tgaccgcggc cgaggacatc ggcgcgggcg gacagccttt ggtcgtcaat ccaggcggcc
    11581 agctaatcgt ttcggcgggc aacgacattt acctccgcgt cccgggcagc ctggcgacga
    11641 gcaagttcct ggccctcacg tcgaccgccg tcgacggctt cgtctcgctc agctccggcg
    11701 ccgtcacggt ggatgatgcg agcggcttcg atctcgataa tgatacgctc gagatcatct
    11761 ccacggtccc ggtgaccggc gacattacga tcaattcgtc gctctccgcc ggcagcgtgc
    11821 tcctgcaagc ggccggctcg atcgttagca gcacgggcgg gcccgacgtt acgacgaccg
    11881 atggcggcac gggcggcgac atcgttctgt ttgccagccg cggcatcggt gccagcggca
    11941 acgcgatcgt gctcaatccc agcgccgcgg gcgacattac ggctgagacc gccggcatca
    12001 atacccccgg cgacccgacg gcgggcgaca tctacctgag ctccagcagc agcttccggc
    12061 tgagcgggat cgtcactgac ctgagctcga cgcagatcgt ctcactcgag accaccggcg
    12121 gtagtttcac gatcgccgag gcgagcaaca ccaacgacca ctggattctc aagtcgtccg
    12181 ccgcgattgc gttcagcggc gacggcacga ttcgcgccca cgaagtggaa ttggacgcga
    12241 ccggcgcgat taccagcacc tctaccgcgc ccgtcgatat cgacaccagc gcgggtgggc
    12301 cgacgggcac gggcggggat atcaagttga gcggcacggc cctgggcgcc agcggcgatt
    12361 ccctggttct gactcccggt ttgggcaatc tggaagccac cgcgactacc ggcggcatct
    12421 tcctggagtt tccgcagaac ctgtcgctga gccagttcac caagctcgac ttgcagggta
    12481 ctagcggcac gctcgacctg agcgtgacca gcggcgacct ggtgctcgac aagtcggtcg
    12541 gcgattcgac aaaatcgcag acatacagcc tctctgcggc cggcaatatc actttcgagg
    12601 acgaagctgc caatgaccca ccgacgctga ccgcgaacat cattaacctg acggcggggg
    12661 ccgacatcct caacgaatcg acgcagccag atctgccgcc gcccgagaat cccttccagt
    12721 cctatcagcc gacggcgaac ctgcaggcca cgaccagcat caccctcgtg gccgacaacc
    12781 tgggccaggc gagtgacccc ctgcaagtcg cgcccggtac cggcaatctg actctcgtcg
    12841 ccagcggcga cacctgggtg aacgtcgtgg ggggcggcat cgatgccacc aagctcagcg
    12901 tgcaggggag ccagaccgtc ggactgtcca ccagcg
//
LOCUS       DASRJB010000141         7598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3143097, whole genome shotgun sequence.
ACCESSION   DASRJB010000141 DASRJB010000000
VERSION     DASRJB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143097"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFB80_03995"
     CDS             complement(<1..264)
                     /locus_tag="VFB80_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32950.1"
                     /translation="MSDLTDALEHAAWMRRAHRWTLGLAVQLAVNCAMVALALGGFGA
                     SRPEFPAAIPSAAPVTESAPPALRPAPTEDPTPTQPLTPAGEPA"
     gene            445..1575
                     /locus_tag="VFB80_04000"
     CDS             445..1575
                     /locus_tag="VFB80_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32951.1"
                     /translation="MNRDDQTQPLLTVPFAPREPARVTEPLPTKTEPARPGTITETHL
                     TQPLATEAFVTQPLPTVRLLPRPEATSQAPSTLTELAGELPQLSPPSLTEPVCETSQG
                     SPPAPLIAAETAPLTEPASVALALEEPAHEAPVSEGPAAAEPQPAIRDPFDSEPLREL
                     ERLSAQIEQELRKSNPAYRPVPVQPEPPAEAAEEPLELYLERFMERVTGKKLDTPTTP
                     AAPPVAATPSPVAPQAAPEPAARRTALPAKDLVPTTKHTERPAIASPAPPAAAALPPR
                     ESSRPPERREQLAAMRDLANESTRSAFAEHAGRKQWQEAHIAFMTAMAASIFSSLYAV
                     WHMATGAPGAIQSAVCAGLLAALLACRFFLRCRQLCGTTRKN"
     gene            1773..2441
                     /locus_tag="VFB80_04005"
     CDS             1773..2441
                     /locus_tag="VFB80_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32952.1"
                     /translation="MKPLPTHFLFRSALACCAVSLACSATSAGEPLYTVAPLAEFPAP
                     PASDLAVAVGPAEPFSPPPIPPWPGADLWEDYHRPIAGRGLYGPPCKTCRGHQQPLVS
                     LLEGFLSLLTFPSRKAAAHRHGHSKALADCGCQPPATPPADWNPDQPPRNVIPRPVPP
                     PEGEGEKPSAEPQRLYPGRAPVVELAPDPVLPPSSVQPPQNEIPTPGDRPPRNVIPRR
                     PNRP"
     gene            complement(2529..2807)
                     /locus_tag="VFB80_04010"
     CDS             complement(2529..2807)
                     /locus_tag="VFB80_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32953.1"
                     /translation="MRTLAILFAVLLCSCVQATEPEKLTAENFKKIKLGASYEFVYTL
                     IGEESVSEYEYGTTRCLVWRVKEQDHTQKYIRLMFERDKVTGKWQVGL"
     gene            complement(2917..3870)
                     /locus_tag="VFB80_04015"
     CDS             complement(2917..3870)
                     /locus_tag="VFB80_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HZN32954.1"
                     /translation="MKSARLAFLLLGVFAAQARHLVAAADGLQVAAHDDRVVITHGEQ
                     PVATWFQGGDTGPVGPYFAHVHAPGGISVTRHFPPRAGIDAADHDTMHPGIRLSFGDL
                     DGVDFWRNKAGVRRVGSPERPAVKDGVASFAATNVYYQGPRTVCREECRYTVRVLGEG
                     YLLTMHSRFSPAGEMPLAFGDQEEMGLGVRVATPLTVKAGGTITNSLGGKNEKEVWGK
                     QADWCDYSGTASGRRVGMLLIPHRDNFRRSWFHARDYGFVAANPFGQQAFTRGDKSRV
                     EVKPGEHFDLRFGVWVYSTPAATPPDLAAIAAEYQRLAPGR"
     gene            complement(3905..4045)
                     /locus_tag="VFB80_04020"
     CDS             complement(3905..4045)
                     /locus_tag="VFB80_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32955.1"
                     /translation="MTAILITAMVSPLVNCPRLLAWPKLYRRPPQNANPRRAVAIGAR
                     RL"
     gene            4097..4357
                     /locus_tag="VFB80_04025"
     CDS             4097..4357
                     /locus_tag="VFB80_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32956.1"
                     /translation="MASRGWQHDEAAAVVEEETIKLTFKHERANVKVELRQGSKEVRV
                     SLDCENLQFTGTQATEKVELAAGTVWGIIALPGGGCLPVQLY"
     gene            complement(4373..5098)
                     /locus_tag="VFB80_04030"
     CDS             complement(4373..5098)
                     /locus_tag="VFB80_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32957.1"
                     /translation="MRRHVASLRAVSRPAQGGESPLALAATPVLRYSDPGGITTDASV
                     WVWGGPGRPAIVAGIFFLTQEGRDPKWSCELLSLADAGVAVHSRVGWSWTPDKSDLAW
                     QTLEDRPGDSDRQRLRQMKEIALRFEVSTFEGTNKTQLRLMVQPLCRYADEQRGLLGG
                     AIFSFAAGTNPEVLLLVECRKNGEALQWHAAFTRFGANPCQARRGEAVVWERPAIQRW
                     DPKEPYFSQFGPVERVFEAAAAK"
     gene            complement(5216..6040)
                     /locus_tag="VFB80_04035"
     CDS             complement(5216..6040)
                     /locus_tag="VFB80_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32958.1"
                     /translation="MATTLLACVLVLAQADAPAREAAAKEQQRKWNEYYLKVAADYEI
                     KRGKEQAQKLALKPGAVLFWSNPIRPGETNGSVFVWTYEGQAELVGTIFSHLVRGSTD
                     TKIIAHEFQSLTRQPLAASRPGAPGTWSLVGDGLEARPIPDAPPPASTAAGRLSQMRN
                     LAREFSATTNLEGVEQELRLLTQPIHRNEKSVGEILDGALFTFVTGTDPELMLVIEAR
                     PAAGGDKPAWHYAAGRFTDLTLKLRHNGRELWTFERGTTPDPYTCLRPETLPKIMP"
     gene            6140..6976
                     /locus_tag="VFB80_04040"
     CDS             6140..6976
                     /locus_tag="VFB80_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32959.1"
                     /translation="MKDTKWLAVGIVLGLVAPLRALPAQDDEAAVSRKRLALMEQTIA
                     SFVVKSDQIAAADRLKFPGVPLLRYSDPTRGTTEENVLIDATVWRLGAQGRPTGLVTL
                     EIYRSTAETGILAYEFSSFVSDRFSLSGKTSAAVVWEAAGSALTLRPLGGAPPPARTA
                     ATRLVQLRQLARRFTVRETLKGDTIECRLLTAPLDRYQASADGIEDGALFAFANGTNP
                     EVVVALECGPKSWTYGLARLSAAETAVHLDGREVMHYDKVDNARLRGDFTSRIERIEL
                     PK"
     gene            6998..>7598
                     /locus_tag="VFB80_04045"
     CDS             6998..>7598
                     /locus_tag="VFB80_04045"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN32960.1"
                     /translation="MQRANFIAFVVLNLLLNSARADEPQPAPEGFEAIFNGKDLSGWE
                     GSADYWSVTGGCLTGVTDGSLKYNRFIMWRGGTLKNFELRVQVKVTPGGNSGLNYRSK
                     HRPDLGDGVVTGYQCDVVPGRADYNGMLYEERGRRILAHTGERVIIDTEGQPWVVDKL
                     PTREFKAGEWHDFRVLVEGNHHQHWIDGHQTVDVIDLDEK"
BASE COUNT         1278 a         2564 c         2499 g         1257 t
ORIGIN      
        1 ggccggttcc ccggctggcg tcagcggctg cgtcggcgtt gggtcttctg tgggcgcggg
       61 acgcaaggcc ggcggagcgg attcggtcac cggcgcggcg gaggggatgg ccgcgggaaa
      121 ctccggccgc gatgcgccga agccgcccag ggccagcgcg accatggcgc agttgaccgc
      181 cagctggacg gccaggccga gcgtccagcg gtgcgcgcgg cgcatccaag cggcgtgttc
      241 cagggcgtcg gttagatcgc tcatcgtcgg tcaatggcag gaggatcgtc gcactgtgta
      301 aacgtaggtt gccccccaag agttgccggg ggccagtcgc acaatcggca ataccggttg
      361 tgacggtcgc agcagctcat tggacggaga cccggtcgcg atgtagatgt tggcagacac
      421 tctacgcaca cgacgaagtc tgtcatgaac cgcgacgacc agacccagcc tttgctcaca
      481 gtaccttttg ctccccgaga gccggcgagg gtcaccgagc cgctgcccac gaagaccgag
      541 ccggcgcgtc cgggaaccat tacggagacg cacttgacgc agccgctcgc gacggaggcc
      601 ttcgtcacgc agccgctccc cacggtgcga ttactgccac ggcccgaggc tacctcgcag
      661 gcgccctcca cgctcaccga gctggctggc gaattgccgc agctgtctcc gccttcgctt
      721 accgaaccag tctgcgaaac atcccagggc agccctcctg cgccgctaat cgctgccgag
      781 acggccccgc tgaccgaacc ggccagcgta gcgcttgccc tcgaagagcc tgcccacgaa
      841 gcaccggtga gcgaagggcc agcggctgct gagccccagc cggcgattcg cgaccccttc
      901 gattccgagc cgctgcgcga actcgagcgg ctgagcgcac agatcgagca ggagctgcgg
      961 aaatccaacc ccgcgtaccg gccggtgcca gtgcagccag agccgccggc ggaggctgcg
     1021 gaggagccgc tggagttgta tctggagcgg ttcatggagc gggtgacggg caagaagctc
     1081 gacacgccga ccacgccggc tgctccgccg gtcgctgcga ctccttcgcc agtcgcgccc
     1141 caggcggctc ctgagcccgc cgcccgacgg acagcgctgc ctgccaaaga cctggtgccg
     1201 acgacgaaac atacagaacg gcccgccatc gccagccccg cgccgcctgc ggcggcggca
     1261 ttgccgccgc gcgagtcctc gcggccgccg gagcgccgag aacagttggc cgccatgcgc
     1321 gacctggcca acgagagcac ccgctccgcc tttgcggagc atgccgggcg gaagcagtgg
     1381 caggaggcgc acatcgcctt tatgaccgcg atggccgcgt cgatcttttc gtcgctgtac
     1441 gcggtctggc acatggcaac cggcgctccc ggcgcgatcc agagcgccgt ctgcgcgggg
     1501 ctgttggcgg cgctcctggc gtgccggttt ttcctgcggt gccggcagct ctgcgggaca
     1561 acccgcaaga actgagcgac cggaagagcg cctcggggcg gttagtagcg tggctccaga
     1621 tttgcctgcg gcgctcaaag tgccttcgct agcggttgtt tcgcgaggcc ccaagttaat
     1681 ggtggtgcgg cgggccggca gacgatgatt tgtccgcacg gatggtgcga tcagcgcggc
     1741 ggctgatcgc ggctcgactg acaggctcac ggatgaagcc tctacccacc cactttctgt
     1801 ttcgcagtgc gctcgcgtgc tgcgcagtat cgctggcctg ctccgcgacc agcgccggcg
     1861 agcccttgta cacagtggcc ccgctggccg agtttcccgc acccccggcg agcgacctgg
     1921 cggtggcggt ggggccagcg gagccctttt ccccaccccc gatcccgccc tggcccggcg
     1981 cggacctgtg ggaagactac caccggccga tcgccggccg cggcctgtac ggaccgccct
     2041 gcaagacctg ccgcggccac cagcagccgc tggtctcgct gctggaaggc tttctgtcat
     2101 tgctcacgtt tccctcccgc aaagcggcgg cgcatcggca cggccacagc aaggcgctgg
     2161 ccgactgcgg ctgccagccc ccggcgacgc ctccggcgga ttggaatccc gaccagccgc
     2221 cgcggaatgt gattccccgg cccgtgccgc cgccggaagg cgagggcgag aaaccatcgg
     2281 ccgagccgca gcggctctat ccgggccgcg cgcccgtggt cgagttggcc cccgacccgg
     2341 tcctgcctcc cagctcggtt cagccgcctc aaaacgagat tcccacgccg ggcgaccggc
     2401 cgccccggaa cgtgatcccg cggagaccga acaggcccta accgggtgag ttttgaattg
     2461 aggtctttgg cctcatgtcg aatcagacgc gcgtggctcc tcttccagga tgcgcgaacg
     2521 atacgcgctc agaggccgac ctgccacttg cccgtgacct tgtccctttc gaacatcagc
     2581 cggatgtatt tctgggtgtg gtcctgttct ttgacgcgcc agacgaggca cctggtcgtg
     2641 ccgtactcat actcgctgac cgactcttcg ccgatcagcg tatagacgaa ctcgtagctc
     2701 gctccgagct tgatcttttt gaagttctcg gccgtaagct tttccggctc cgtcgcctgg
     2761 acgcaggagc agagcagcac ggcgaacaga atagccaggg tgcgcatagg tacctccgga
     2821 ttcatgcatc ctagttcgag ttcgccaccc gagggcggag gtatctgccg aatattcgga
     2881 acgactggcg cttctgcccc agcccctaaa ggcagatcat cgccccggcg cgagccgctg
     2941 gtattcggcc gcgatggccg cgaggtcggg cggcgtggcg gcgggtgtgg aatagaccca
     3001 gacgccgaac cgcaggtcga agtgctcgcc cggcttgacc tcgacgcggc ttttatcgcc
     3061 gcgggtgaag gcctgctgtc caaagggatt cgccgcgacg aagccatagt cgcgggcatg
     3121 aaaccacgag cggcggaagt tgtcccggtg gggaatcagc agcatgccca cgcggcggcc
     3181 actggcggtt ccgctgtagt cgcaccaatc ggcttgcttc ccccacactt ctttctcgtt
     3241 cttgccgccc aggctgttgg tgatcgtgcc gcccgccttg actgtcagcg gcgtcgccac
     3301 gcgcactccc aggcccatct cttcctgatc cccaaaggcc agcggcattt cgcccgccgg
     3361 ggaaaatcgg ctgtgcatcg tgagcaggta gccctcaccg agcacgcgca cggtgtagcg
     3421 gcactcctcc cggcagaccg tgcgtggccc ctggtaatag acgttcgtcg cggcaaaact
     3481 tgccacgccg tccttcaccg ccggacgctc cgggctgccg acgcggcgca cgcctgcctt
     3541 gttccgccag aaatcgactc catccaggtc gccgaacgag aggcggatgc cggggtgcat
     3601 cgtgtcgtgg tccgcggcat cgatgcccgc ccgcggtgga aaatgacgcg tgaccgaaat
     3661 gccgcccggc gcgtgcacgt gggcgaaata aggcccgact ggacccgtat cgccgccctg
     3721 aaaccaggtc gccaccggtt gctcgccgtg cgtgatgacc acgcgatcgt cgtgagcagc
     3781 gacttgcagg ccatcggccg cggcgaccag gtggcgcgct tgcgcggcga aaaccccgag
     3841 cagcaggaag gccaggcggg cggatttcat gcggcaatct cctggtggca tggcagccga
     3901 taagctacag ccgccgggcc ccgatcgcca cggctcggcg cggattggcg ttttgcggcg
     3961 gccgccgata caacttgggc caggcgagta gccgcgggca attcacgagg ggactcacca
     4021 tggctgtgat caggatggcc gtcataggga tcgcgctggg cctcgtgttt gccgcggcag
     4081 ggccgggcag aaggagatgg cctcccgcgg atggcagcac gacgaggcag ccgcggtggt
     4141 cgaagaggag acgatcaagc tgacgttcaa acacgagcgg gcgaacgtga aagtggagct
     4201 gcggcaagga tcgaaggagg tgcgggtcag cctggattgc gaaaaccttc agttcaccgg
     4261 gacccaagca accgagaagg tcgagctcgc cgcaggcacg gtgtggggca tcatcgcgct
     4321 gcccggcggc ggctgcctgc cggtgcaact ctactgagaa gatccggcac tgctatttcg
     4381 cggcagctgc ctcgaacacg cgctcgaccg gtccgaactg cgagaagtac ggttccttgg
     4441 gatcccaccg ctggatggcg ggacgttccc acacgacggc ctcgccccgg cgagcctggc
     4501 agggattcgc gccaaaacgg gtgaaggcgg cgtgccactg gagtgcttcc ccgtttttgc
     4561 ggcactccac cagcagcaga acttcggggt tcgttcccgc agcgaacgag aagatcgcgc
     4621 cgcccaagag cccccgctgc tcgtcggcgt agcgacagag cggctgcacc atgagccgca
     4681 gttgcgtctt attagtgccc tcgaacgtgg agacctcgaa ccgtagagcg atctctttca
     4741 tctgcctcag gcgctgccga tccgaatctc cgggccgatc ttccagcgtc tgccacgcga
     4801 gatcgctctt gtccggcgtc caactccagc cgacgcgcga gtggacggcc acgccggcgt
     4861 cggccagcga caacagttcg cagctccact tcgggtcgcg gccttcctga gtcagaaaga
     4921 agatccccgc gacgatcgcc ggccggcccg ggccgcccca gacccagacc gaggcgtcgg
     4981 tcgtgatgcc gcccggatcg ctgtagcgga gcaccggagt cgcagctagc gcgagcggtg
     5041 actcgccgcc ctgagccggg cgactgacgg cgcgcagcga cgccacatga cgccgcatgg
     5101 tctccagggt ctgacttgaa acgtccctct cttgggccgc tgccgaccac gcgcagacca
     5161 acccccaggc aactgacagg acgccgagct ggaatcgtcg catgggaaat ccctgctagg
     5221 gcatgatctt tggcagcgtc tccgggcgga ggcacgtgta gggatcgggc gttgttccgc
     5281 gctcgaacgt ccacagttcc cgcccgttgt gccgcagctt gagcgtcagg tcggtgaacc
     5341 gcccggcggc gtagtgccag gccggcttgt cgccgccggc ggcaggccgg gcctcgatca
     5401 ccagcatcaa ctccgggtcg gtgccggtga caaaggtgaa tagcgcgcca tcgaggatct
     5461 cgccgacgct cttttcatta cggtgaatcg gctgggtcaa aagccgcagc tcttgctcga
     5521 cgccctccag gttcgtcgtg gccgagaatt cgcgggccag gttccgcatt tgcgacaagc
     5581 gcccggcagc cgtgctcgcc ggcgggggag cgtccgggat cggcctggcc tcaaggccgt
     5641 cgcccacgag cgaccacgtg ccgggcgctc ctggccggct ggcggccagc ggctggcgcg
     5701 tgagcgactg aaactcgtgg gcgatgatct tggtgtcggt gctgccccgg accaggtgcg
     5761 agaaaatcgt ccccaccaac tccgcttgcc cttcgtaggt ccagacgaac actgatccgt
     5821 tcgtctcgcc cggccggatc gggttgctcc agaagagcac cgccccgggc ttcagcgcga
     5881 gcttttgcgc ctgctccttg ccccgcttga tctcataatc cgccgccacc ttcagatagt
     5941 actcgttcca cttccgctgc tgttccttgg cagccgcctc tcttgcgggc gcatccgcct
     6001 gggccaaaac gagcacgcaa gcaagcaagg tcgttgccat ggtcgcctcc actctagact
     6061 ggcgagtata gacgtagcgt ctggatttgc aaccggcgct tggggtatat tgcgctcgtc
     6121 gtattgggag ggttgcacca tgaaggacac gaaatggctt gctgtgggca tcgtgctggg
     6181 cctcgtcgca ccgctgcggg ctctgccggc acaagacgat gaggcggccg tcagccgcaa
     6241 acgcctggcg ctcatggagc agacgatcgc gtcgttcgtg gtgaagtcgg atcagatcgc
     6301 cgctgccgac cggctgaagt ttcccggcgt gccgctgctg cggtacagcg atccgacgcg
     6361 cggcacgacg gaagaaaatg tgctgatcga cgccacggtc tggcggctgg gagcgcaggg
     6421 ccggcccacg ggcctggtga cgctggagat ctatcgcagc acggccgaga cgggcatcct
     6481 ggcttacgag ttctcgtcgt ttgtttcgga ccggttttca ctctcaggca agacgagcgc
     6541 ggcggtcgtc tgggaggctg ccggctcggc gctcacgcta cggccgttgg gcggagcccc
     6601 gccgccggcg cggaccgccg cgacccggct ggttcagctg cggcaattgg cccgccgctt
     6661 caccgttcgc gagacgctca agggggacac gatcgagtgc cgcctgctca ccgccccgct
     6721 cgaccgctac caggcctcgg ccgacggcat cgaagacggc gcgctcttcg cctttgccaa
     6781 cgggaccaat cccgaggtcg tcgtggcgct cgaatgcggc ccgaagagct ggacctacgg
     6841 tttggcccgt cttagcgcgg ccgaaaccgc cgtccatctc gacggccgcg aggtcatgca
     6901 ctacgacaag gttgacaacg cccgcctccg cggcgacttc acctctcgta tcgagcgcat
     6961 cgagctgcca aagtgagagg ctttccggag cattgtcatg cagcgagcca acttcatcgc
     7021 gttcgtcgtc ttgaatctcc tgctgaattc tgcccgggcc gacgagccgc agccggcgcc
     7081 cgagggcttt gaggcgatct ttaacggcaa ggacctcagc ggctgggaag gcagcgcgga
     7141 ctattggagc gtcactggcg gctgcctgac cggcgtcacc gacggctcgc tgaagtacaa
     7201 ccggttcatc atgtggcgcg gcggcacgct gaagaatttt gaattgcggg tgcaggtaaa
     7261 ggtcacgccc ggcggcaata gtggcctgaa ctatcgcagc aaacaccgcc cggacctggg
     7321 cgacggcgtg gtcaccggtt atcagtgcga tgtcgtcccc ggccgcgccg actacaacgg
     7381 aatgctctac gaagagcgcg gtcgccgtat cctggctcac accggcgaga gagtgatcat
     7441 cgacacggaa ggtcagccgt gggttgtcga taagcttcct acaagagaat tcaaggccgg
     7501 cgagtggcac gacttccgcg tgctcgtgga gggcaatcac caccagcact ggatcgacgg
     7561 ccaccaaacc gtcgatgtca tcgacctcga tgaaaaga
//
LOCUS       DASRJB010000142         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3707195, whole genome shotgun sequence.
ACCESSION   DASRJB010000142 DASRJB010000000
VERSION     DASRJB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707195"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFB80_04050"
     CDS             <1..588
                     /locus_tag="VFB80_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HZN32961.1"
                     /translation="EMTSQVVRKRDPLERVGNVASVGGKVQVIEYSDLPDEFARRTNA
                     DGSLKLWAGNLAVHAMDVAFLARATAQKNALPFHLAKKKVPYVGEDGQIVEPDKPNAI
                     RFERFIFDLLPLAARALVVEADPAEAFAPVKNSDDEPTDNPRLARAAMMAQARRELRS
                     RGAKIADNVPVELNPLWDGQLPPGTVITQPTYFAR"
     gene            657..1742
                     /locus_tag="VFB80_04055"
     CDS             657..1742
                     /locus_tag="VFB80_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN32962.1"
                     /translation="MLHAVIMAGGSGTRFWPLSRMVRPKQLLDLAGGRTMIQATVDRL
                     GNLAPAERTWIITNRSLASAIAEQLPQLARERIVGEPCKRDTAPAVGLSALLVAASDA
                     DATLVVMPSDHLIEPAAAFQAALQQAAAMVEERPQTLVTFGIKPTHPAESFGYIERGK
                     ASGRREPPDSAAYEVARFREKPARSVAEQYLASGNFYWNSGIFVWKAKTILAALAKFE
                     PAIYERLVAIGAAIGKPDFEPTLDREFTAIRGKSIDFAVMEHYQPVVVIEAPFTWDDV
                     GSWQALARARGVDDAGNTAVGKHLGIRTSGSIIRTTPDHLIVTLGLKDTIVVHTEGAT
                     LVADKSQEEAIREVVKALEERGWREFL"
     gene            complement(1793..2185)
                     /locus_tag="VFB80_04060"
     CDS             complement(1793..2185)
                     /locus_tag="VFB80_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32963.1"
                     /translation="MQFKAAAVGLLAASLLAGCGDSGPPRYHLAGKVTFRGKPIPAGI
                     IYFDPDLAGGHDGPQGFASIKDGAFDTRSGGQGHAGGKCVVRIFGTDGIPAPEANMGK
                     NLFSEYALNVELPTQDSTKDFVVPPRGR"
     gene            complement(2234..3190)
                     /locus_tag="VFB80_04065"
     CDS             complement(2234..3190)
                     /locus_tag="VFB80_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN32964.1"
                     /translation="MTCPLRARRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRM
                     SCQNNLKQIGIAIHNYIDVVNAFPPGAVKSNTTSWHVHVLPFLEQKNLYDQFDFTQGD
                     YIASGGVGRANLGLNRVNTYLCPSSAAVKMMLPDPPNNVNPPDLIPANTGVPPYTTHY
                     YGNMGPKPPSLPGTGYTFRNVGQGGFSQHGLFEVNSRIRLAEVTDGTSNTILVGENSR
                     HVQSLGSRFRNWMRGADYNSNQNSAGTDHICGCRNIQLGINTPTPALSTTFNDIAMAS
                     HHPNGANFVLCDGAVKFLTQNIELGNYKALASRDGNETTQVD"
     gene            3461..>4828
                     /locus_tag="VFB80_04070"
     CDS             3461..>4828
                     /locus_tag="VFB80_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN32965.1"
                     /translation="MLRIHSSPKNLCGGMSRRDWLRVGGLGLAGLTLPQVLLLQEAAA
                     AENVPRERSFGRAKSIILIHLYGSPSQLEWVDPKPDAPVEIRGQFGVIPSSLSGCNVC
                     DLFPQMAKVMDRTTVLRSLTHPYPIHGVAYALTGVPAIDVAMELSPHDSRHWPYFGSV
                     VEYLASGKRELPDIPVPRAPPTHREAHASRSPATLPTNIALPFRFSTQRIGEVPRAGP
                     YAAFLGSEFDPLWADFRGTPTKGITKTLRDMTYTDHDPYVGVADGSYFIVPDASQLQP
                     ELTLDRLDTRKSLLSQLNDGLPSLVASTAGRALTKHQQAALSLLESPRLAAALDLRKE
                     DAATRELYGQSLFGQSCLAARRLVEAGSKVVSVFWDEYGLAGSGWDTHWDHFNRMKLE
                     LSPGFDQGWYGLITDLDRRGLLNDTLVVCTSEHGRTPQINKAQGGGRDHWSRVYSSLV
                     AGGGTA"
BASE COUNT          815 a         1564 c         1612 g          837 t
ORIGIN      
        1 gagatgacgt cccaggtcgt acggaagcgc gatccgctgg aacgcgtggg aaacgtcgcg
       61 tcagttggcg gcaaggtgca ggtcatcgag tacagcgact tgcccgacga attcgcccgg
      121 cggaccaatg ccgacggcag cctgaagctg tgggcaggca acctggcggt gcacgcgatg
      181 gacgtggcct tcctggcccg cgcgacggcc cagaagaacg ccctgccgtt tcacctggcg
      241 aagaagaagg tgccctacgt cggcgaggac ggccagatcg tcgagccgga caagccgaac
      301 gcgatccgct tcgagcggtt catcttcgac ctgctgccgc tggccgcgcg ggcgctggtc
      361 gtcgaggccg atccggccga ggcgtttgcc cccgtgaaga actccgacga cgagccgacc
      421 gacaacccgc ggctggcccg cgctgcgatg atggcccagg cccgccgcga gctgcgctcc
      481 cgcggcgcga aaatcgccga taatgtgccc gtcgagctca acccgctgtg ggacggccag
      541 ctgccgcccg gcacagttat tacgcagccg acgtattttg ccaggtagcc gaattcgcca
      601 gaattcggag tgcatcacca acccgaattc tggcgaattc ggctacggaa cgtgcaatgc
      661 tccacgcagt catcatggcg ggcggcagcg gcacgcggtt ctggccgctc agccggatgg
      721 tccgtccgaa gcagctgctc gacctggcgg gcggccggac gatgatccag gcgacggtcg
      781 atcgcctggg caacctcgcg ccggccgagc ggacgtggat catcaccaac cgcagtctcg
      841 caagcgccat cgccgagcag ctgccgcagc tggcccgcga gcggatcgtc ggcgagccct
      901 gcaagcgcga caccgcgccg gccgtcggtt tgtccgcgct gctggtggcg gccagcgacg
      961 ccgatgccac gctggtcgtg atgccgtcgg atcacctgat cgagccggcc gcggcctttc
     1021 aggcggcact gcagcaggcg gcggcgatgg tcgaagaacg cccccagacg ctcgtcacgt
     1081 tcggcatcaa gccgacgcat cccgcggagt cgttcggcta catcgaaagg ggcaaggcga
     1141 gcgggcggcg tgagcctccc gatagcgctg cctacgaggt cgcccgcttc cgcgaaaagc
     1201 ccgcccgttc ggtcgccgag cagtacctcg ccagcggcaa cttctactgg aacagcggca
     1261 tcttcgtctg gaaggccaag acgattctcg cagcgcttgc caaattcgag ccggcaatct
     1321 acgagcggct ggtcgcaatc ggcgcggcga ttgggaagcc agacttcgag ccgacgctgg
     1381 accgcgaatt tacggccatc cgcgggaagt cgatcgactt cgcggtgatg gagcactatc
     1441 agcccgtcgt ggtgatcgaa gctccgttca cctgggacga tgtcggcagt tggcaggccc
     1501 tggcccgcgc ccgcggcgtc gatgacgccg gcaacaccgc cgtgggaaaa cacctgggca
     1561 tccgcaccag cggctcgatt atccgcacga cgcccgacca cttgatcgtg acgctcggcc
     1621 tgaaagacac gatcgtcgtc cacacggagg gcgcgaccct cgtcgccgac aagagccagg
     1681 aagaggccat ccgcgaagtc gtcaaagccc tcgaagaacg gggctggcgg gagttcttgt
     1741 agtggggcag acagttctgt ctgccatagc aagctgaagc ccgccccacg cttcagcgtc
     1801 cgcgtggcgg aacgacgaaa tccttcgtcg agtcttgcgt tggcaattcg acgttgagcg
     1861 cgtattcaga aaagaggttc ttgcccatgt tggcttccgg cgcgggtatt ccgtcggtgc
     1921 caaagatccg caccacgcac ttgccaccgg cgtgtccttg gccgccgctc cgcgtgtcga
     1981 aggctccatc cttgatcgag gcaaaccctt gaggcccgtc atgtccgccc gcgaggtcgg
     2041 ggtcgaaata gatgatgccc gccgggattg gcttgccgcg gaaggttacc ttgccggcca
     2101 ggtgataccg cggcgggccg gagtctccgc agccggcgag caggctcgcc gcgagcaggc
     2161 cgacagccgc cgctttgaat tgcatggatc gtggatcggg caaggcgttg tttgacgggg
     2221 gaccgcgatg gactcaatcc acctgcgtcg tttcgttgcc gtcgcggctg gccagcgcct
     2281 tgtaattgcc cagctcgata ttctgcgtga gaaacttgac tgccccgtcg cacagcacga
     2341 agttcgcacc gttcggatga tggctggcca tcgcgatgtc gttgaaggtc gtgctgagcg
     2401 cgggagtggg cgtgttgatt ccgagctgga tgttgcggca gccgcagatg tggtcggtcc
     2461 cggcgctgtt ctgattggag ttgtaatcag cgccccgcat ccagttgcgg aatcgcgagc
     2521 cgaggctctg cacgtgccgg gagttctcgc cgacgaggat cgtattcgac gtgccgtcgg
     2581 tgacttcagc caggcggatg cggctgttaa cttcgaacag gccgtgctgg gaaaagccgc
     2641 cctgcccgac attgcggaac gtgtagcccg tgcccggcag cgacggcggc ttaggtccca
     2701 tgttgccgta gtagtgagtc gtatagggcg gcacgccggt gttcgccgga atcaggtccg
     2761 gcggattcac gttgttgggt ggatcgggca gcatcatttt gacagccgcc gaactgggac
     2821 acagataggt gttgacccgg ttgagcccca ggttcgcgcg gccgacgccg ccgctagcga
     2881 tgtagtcgcc ctgcgtgaag tcgaactggt cgtacaggtt cttctgctcc aggaacggca
     2941 gcacgtggac atgccagctg gtcgtattcg acttcaccgc tccgggagga aacgcgttga
     3001 cgacatcaat gtagttgtgg attgcgatac cgatttgctt caggttgttc tggcagctca
     3061 tccgccgtgc cgcttctcgg gcggcttgga cggcgggcag cagcagggcg accaggacac
     3121 cgatgatggc gatgacgacg agcagctcga cgagcgtgaa cgcccgccgg gcgcggagag
     3181 gacaggtcac gagggactcc cggggtgtgc agaggcggcc tcaatcaatt cctgggattt
     3241 cattagaatc cagcgcatgt gggtatttca accctcgctg gacctgcgag tttcactcat
     3301 tcttgcggca tttcgcaagg gtttgcagcg tcgcggcgac cgcctccaag ggggcggact
     3361 ttgccccaag attcttcgcg ggacaactgg caatccgggg cgcgttttcc gataatcaac
     3421 cggcgtcgat gattgcccgt cccgcgaggc gattgaccgt atgcttcgca tccattcctc
     3481 gccgaagaac ctgtgcggcg gcatgtcgcg gcgcgactgg ctgcgcgtcg gcgggctggg
     3541 actggccggg ctgacgctgc cgcaggtgtt gctgctgcag gaagcggcgg cggccgagaa
     3601 cgtcccgcgg gaacggtcgt tcgggcgggc caagtcgatc atcctgattc acctctatgg
     3661 ctcgcccagc cagctggaat gggtcgatcc gaagcccgac gccccggtgg aaatccgcgg
     3721 gcagttcggc gtgatcccgt cgtcgctgag cggctgcaac gtctgcgacc tgtttccgca
     3781 gatggcgaag gtgatggacc gcacgacggt gttgcggtcg ctcacgcatc cgtatccgat
     3841 ccacggcgtg gcctatgccc tcacgggcgt gccggcgatc gacgtggcga tggagctctc
     3901 gccgcacgat tcgcgccact ggccgtattt tggatcggtg gtggagtatc tggcgagcgg
     3961 gaagcgtgag cttcccgata tcccagtacc ccgggcgccg ccgacgcatc gggaggctca
     4021 cgcctcccgc tcgcctgcca ctctccccac caacatcgcg ctgcccttcc gcttcagcac
     4081 gcagcggatc ggcgaggtgc cgcgggccgg gccgtatgcg gcgttcctcg gcagcgagtt
     4141 tgatccgctc tgggccgatt tccgcggcac gccgaccaag ggcatcacca agacgctccg
     4201 cgacatgacg tacacggacc acgatccgta cgtcggcgtc gcggacggtt cgtacttcat
     4261 cgtgcccgat gcgtcgcagc tgcagcccga gctgacgctc gaccggctcg acacgcgcaa
     4321 gagcctgctc tcgcagctca acgatggcct gccgagcctc gtcgcctcga ccgccggccg
     4381 ggcgctgacc aagcatcagc aggccgcgct gtcgctgctc gaatcgccgc ggctggccgc
     4441 ggcgctcgac ctgcgaaagg aagacgccgc cacgcgcgag ctctacggcc agtcgctgtt
     4501 cggccagtcc tgcctcgccg cccgccgctt ggtggaggcg ggcagcaaag tcgtcagcgt
     4561 cttctgggac gagtatggcc tggccggcag cggctgggat acgcactggg accacttcaa
     4621 ccggatgaag ctggagctct cgcccggctt cgaccagggc tggtacggcc tgatcaccga
     4681 cctcgaccgc cgcggacttt tgaacgacac cctcgtcgtc tgcaccagcg agcacggccg
     4741 cacgccgcag atcaacaagg cccagggagg cggccgcgat cactggtcgc gggtctattc
     4801 gtcgctcgtc gccggcggcg gcacggcc
//
LOCUS       DASRJB010000143         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_41176, whole genome shotgun sequence.
ACCESSION   DASRJB010000143 DASRJB010000000
VERSION     DASRJB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41176"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFB80_04075"
     CDS             <1..1320
                     /locus_tag="VFB80_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HZN32966.1"
                     /translation="IVWQNLHPLLEDQPGAFLQEVAKLDLGKSPAVTAILPRATERIL
                     ARKKFEAAPVVELFAALRDRAPQSAKQCLELLAGRIQSGELKGEQLAQLKTAFTAPLT
                     PLLTNPESHPVGLDAALVAVSWKDLRAIDYVRRTFTTRGLDPALRLRALAALISAGDE
                     KLLTSVGAALAENNRSREFAGQILAALGRLDSPEVAGVVLASYPKLSADAQPKAIELL
                     TQRAGWAKALMESMAAGKIAPAVLNVNQAQKLIALNDKDLTAAVARHWGTVRTTRDPA
                     REQFVGQMRRLVAGTPGDARRGIAVFNKVCGQCHKIHGQGQDVGPDITANGRASFEQL
                     LSNVFDPSLVIGAAYQARTVRTADGRVITGLLVEDSDQRIVLKVQGGKLETVARGDVD
                     AVKISELSLMPEGLEKQLKPEEIADLFAFILLDKHPDDPAAKRIPDR"
     gene            1357..2541
                     /locus_tag="VFB80_04080"
     CDS             1357..2541
                     /locus_tag="VFB80_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZN32967.1"
                     /translation="MSPTVQTGLERCLLSPPEILRGRRFGLLMNQASVDHRLRYACDL
                     LAEKFPGQLQALFSPQHGLWGEEQANMIESPHGRYQPLDLPVYSLYCETRRPTPEMLR
                     GLDCLVIDLQDVGTRVYTFIWTVQQCLIACAAAGIPVVLLDRPNPIGGQIAEGPLLEP
                     GFESFVGGATIPLRHGLTIGELALLLNSEQQIGASLEVVPLTGWLRGMAFDSTGLPWV
                     WPSPNMPRTTTALLYPGQVLLEGTNLSEGRGTTLPFEVVGAPFIDPHRLLRELEPLDF
                     PGLRLRPIRFVPTFDKWKGQRCGGVALHVEIPFAIRSVTATLQILAAVHRLYRADFAW
                     LPPPYEYERKKMPIDILFGSPALRETFASDSVLAPATIAHLVRLDRHAWSHRAKPYLL
                     KG"
     gene            2552..>5038
                     /locus_tag="VFB80_04085"
     CDS             2552..>5038
                     /locus_tag="VFB80_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN32968.1"
                     /translation="MFRLSFLALAMSCVSPVLGQQPTITAPLGDHNFTLPDGFAIELA
                     AGPPLIDRPVHIDFDEQGRLYATEMNGEITKPDVAAQKPMHRVLRLEDSDGDGRFDKR
                     TVFADQLPFPEGAMWYAGSLYVAAPPKIWKFTDTDNDGVADNRDVWFDAKTLTGCAND
                     LHGPYLGPDGWIYWAKGAFAEQTYTLPSGKTFKTRASHIFRARPDGSGIEPVMTGGMD
                     NPVDVVFTPGGERIFTTTFLQRPAGGNRDGLIHAIYGGIYGKDHDVVYDPVHKWTSPD
                     FMPVLTHLGPAAPCGLHRLESHQFGREFQDNLFCCQFNLRKISRHVLKPSGSTFTTED
                     SDFLVSDNTDFHPTDVIEDADGSLLVVDTGGWYKLCCPSSQLVKGDVLGAIYRIRRVG
                     SHQVADPRGRRIDWEKSSPADLAALLSGERPAVRRRAIERLASLGKAGVEPLAKIVAN
                     PKSPPVAARAGISVAARIDDNFARRLIHEAMRHEDESVVQLAIHAASVNRDATAATQL
                     ARLLDEGSPANRRGAAEALGRMGDQTQVLNLIVAHSRASGDLALEHSLTYSLIELGDG
                     RDVFRYGLTNKSLRTQAAALMALAQMNAELTPPTVLSALESTDPRLRDAACWVAARHS
                     AWGGELSAFFFRRLTERPLAAAEREQLIDLLAKMSRGEAIQRLIGEAAGDFNPEVAKL
                     GLESMAASGFKELPSSWTASLPARLLANSPLLTSALTVVRTIPPAKADADKLAAALTK
                     LADSDGSAEIRLTALVAVPGGLKSVSPSQLELLLAHIDRDQAPTLRGLAAEAISKAKL
                     TGEQLHQLVDKLPRAAPMELDRLLTPFAQST"
BASE COUNT          869 a         1712 c         1582 g          875 t
ORIGIN      
        1 atcgtctggc agaacctgca tccgctcttg gaggaccagc cgggggcgtt tttgcaggaa
       61 gtagcaaagc tcgatctggg caagtcgccg gctgtgaccg ccattttacc gcgggcgacc
      121 gagcggattc tggcgcggaa gaaattcgaa gccgcaccgg tggtcgagct gttcgccgct
      181 ctccgcgacc gcgcgccgca gtcggccaaa cagtgtttgg agctactggc cgggcgaatc
      241 cagtcgggcg agctcaaggg cgagcagttg gcgcaactga agaccgcgtt caccgccccg
      301 ctgacgccgc tgctgactaa tccggaatcg caccccgtcg gcctcgacgc ggcgctcgtc
      361 gccgtttcgt ggaaagactt gcgcgcgatc gactacgtcc gccgcacgtt caccacgcgc
      421 ggtctcgatc cggcgctgcg attgcgggcg ctggcggcgc tgatttcagc cggcgacgag
      481 aagttgctga cgagcgtcgg cgcggcgctc gccgagaaca accgctcgcg cgaattcgcc
      541 gggcagattc tggcggctct cggccggctc gacagccccg aggtcgccgg cgtggtcctc
      601 gcctcgtatc ccaaattgtc agccgacgct cagccgaagg cgatcgagct gctcacgcag
      661 cgagcgggct gggccaaagc cctcatggag tcgatggccg ccggcaagat tgcgccggcg
      721 gtgctgaacg tgaatcaggc ccagaagttg atcgcgctca acgacaagga tctgaccgcc
      781 gcggtggcca ggcattgggg cacggtccgc acgacccgcg acccggcccg cgagcagttc
      841 gtcggccaga tgcggcggct ggtggccggc acaccgggcg acgcccgccg cgggatcgcg
      901 gtgttcaaca aggtctgcgg ccagtgccac aagatccatg gccagggcca ggacgtcggc
      961 cccgacatca ccgccaacgg ccgggcctcg ttcgagcagc tcctctcgaa tgtgttcgat
     1021 cccagtctgg tcatcggcgc ggcgtatcag gcccgcacgg tccgcacggc cgacggccgg
     1081 gtcatcaccg gcctgctcgt cgaagacagc gaccagcgga ttgttctcaa agtgcaaggc
     1141 ggcaagctcg agacagtcgc ccgcggcgac gtcgatgccg tcaagatcag cgagctgtcg
     1201 ttgatgcccg aaggactcga gaagcagctc aagccagaag aaatcgccga cctgttcgcg
     1261 ttcattctgc tcgacaagca ccccgacgat ccggcggcga agcgaattcc tgatcggtag
     1321 tgaaacgcgg cgcaggtctt ctgtccgtcc aaaccaatgt cgccgaccgt ccaaaccggc
     1381 ctcgaacgct gcctgctcag cccgccggaa atccttcgcg gacggcgttt tggcctgctg
     1441 atgaaccagg cctcggtcga tcaccggctg cggtacgcct gcgacctgct ggccgagaag
     1501 tttccggggc aactccaggc gctcttttcg ccgcaacacg gtctgtgggg cgaagagcaa
     1561 gcgaacatga tcgagtcgcc gcacgggcgg tatcagccgc tggatttgcc ggtctacagc
     1621 ctgtactgcg aaacgcggcg gccgacgcca gaaatgctcc gcgggctgga ctgcctggtg
     1681 atcgacttgc aggacgtcgg cacgcgggtc tatacgttca tttggacggt ccagcagtgc
     1741 ctcatcgcgt gcgccgcggc aggcatcccg gtcgtcctgc ttgaccggcc caatccgatc
     1801 ggcggccaga ttgccgaagg gccgctgctg gagcccggtt ttgagagttt cgtcggcggc
     1861 gccaccattc cgctgcgcca cggcctgaca attggcgaac tggcgctatt gctcaatagc
     1921 gagcagcaga ttggcgcatc gctcgaggtc gtaccattga ctggctggct ccgcggcatg
     1981 gcctttgaca gcaccggatt gccctgggtg tggccatcgc ccaacatgcc gcggaccacg
     2041 accgctctcc tctatcccgg ccaggtgctg cttgaaggca ccaacctctc cgaaggtcgc
     2101 ggcacgacgc tgccgttcga ggtcgtcggt gcgccgttca tcgaccccca tcggctgctc
     2161 cgcgagctgg aaccgctcga ctttcccggc ctgcggctgc ggccgattcg cttcgtgccg
     2221 acgttcgaca agtggaaggg acagcgctgc ggcggcgttg ctttgcatgt cgagattccc
     2281 ttcgcgattc gctcggtgac cgcgacgctg caaatcctgg ccgcagttca caggctctat
     2341 cgggccgact ttgcctggct gccgccgcct tacgaatacg agcggaaaaa gatgccgatc
     2401 gacatcctct tcggctcgcc cgctctgcgc gagacgtttg catcggattc ggtactcgcc
     2461 cctgctacaa tcgctcatct ggtcaggctc gaccgccacg cctggtcgca ccgcgctaag
     2521 ccctacctgc tcaaaggctg agaggctatt catgttccgc ctttcttttc tggcactggc
     2581 aatgtcctgc gttagtcccg tgttgggcca gcagccgacg atcaccgccc cgctcggcga
     2641 ccacaacttc acgctgcccg acggctttgc gatcgagctt gccgccggcc cgccgctgat
     2701 cgaccggccg gtgcacatcg acttcgacga gcagggccgg ctgtacgcca ccgagatgaa
     2761 cggcgagatc accaagcccg acgtggccgc tcaaaaaccc atgcaccgcg tcctgcggct
     2821 ggaagactcc gatggcgacg gccgcttcga caaacggacg gtgttcgccg atcagttgcc
     2881 gtttcccgaa ggggcgatgt ggtacgccgg ctcgctctat gtcgccgccc ctcccaagat
     2941 ttggaagttc accgacaccg ataacgacgg cgtcgccgac aatcgcgatg tctggttcga
     3001 cgccaagaca ctcaccggct gtgccaacga cctgcacggg ccgtacctcg gccccgacgg
     3061 ctggatctac tgggccaagg gagcgttcgc cgagcagacc tacacgttgc ccagcggtaa
     3121 gaccttcaag acgcgggcct cgcacatctt ccgagcccgg cccgacggca gcggcatcga
     3181 gccggtgatg accggcggga tggacaaccc agtcgatgtc gtgtttacgc ccggcggcga
     3241 gcggatcttc acgacgacct ttctgcagcg gcccgccggc ggcaatcgcg acggcctgat
     3301 ccacgcgatc tacggcggca tctacggcaa ggaccacgac gtggtctatg acccggtaca
     3361 caaatggacc agccctgact tcatgcccgt gctcacgcac cttggcccgg ctgccccttg
     3421 tggcctccac cggctggagt cgcatcaatt cggccgcgag tttcaggaca acttgttctg
     3481 ctgccagttc aacctgcgga agatcagccg gcatgtcttg aagcccagcg gctcgacgtt
     3541 cacgaccgaa gattccgact ttctcgtcag cgacaacacc gattttcatc cgaccgacgt
     3601 gatcgaagac gccgacggca gcctgctcgt cgtcgatacc ggcggctggt acaagctctg
     3661 ctgccccagc tcgcagctcg tcaaaggcga cgtgctgggt gcgatctatc gcattcggcg
     3721 cgtcggctcg catcaggtcg ccgatccgcg cggccgaagg atcgattggg aaaagtcgtc
     3781 gcccgccgac ttggctgcct tgctctccgg cgaacgcccc gccgttcgcc ggagagccat
     3841 cgagcggctg gcaagcctcg gcaaggcggg agtggagccg ctggccaaga tcgtcgccaa
     3901 tccgaagtcc cctccggtcg ccgcccgcgc cggaatctcc gtcgccgccc gcatcgacga
     3961 caactttgcc cgccggctga tccacgaagc catgcggcac gaagacgaat cggtcgtgca
     4021 gctggccatc catgccgcca gcgtcaatcg cgacgccacg gccgcaacac agctcgcgcg
     4081 attgctggac gaaggttcgc cagccaatcg ccgaggcgcc gctgaagcgc tcggcaggat
     4141 gggcgaccag acgcaggttc tcaacctgat cgtggcccat tcgcgcgcga gtggcgacct
     4201 tgcgctcgaa cattcgctga cctattcgtt gatcgagctc ggtgacggcc gcgacgtctt
     4261 ccggtacggc cttaccaaca agagccttcg cacgcaggcc gcggcactga tggcgctcgc
     4321 gcagatgaac gctgagctga cgccgccgac ggttctcagc gctttggaga gcaccgaccc
     4381 tcggctgcgc gatgcggcgt gttgggtcgc agcccgtcac tctgcctggg gcggcgagct
     4441 ttcggcgttc ttcttccggc ggctgaccga acggccgctg gccgccgcgg aacgcgagca
     4501 attgatcgat ctcctggcca aaatgtcgcg cggcgaagcg atccaaagac tgatcggcga
     4561 agccgccggc gatttcaatc cggaggtcgc gaaactcggc ttggagtcga tggcggcgtc
     4621 gggtttcaag gagctgccga gctcctggac cgcgagcttg cctgctcggc tactcgccaa
     4681 ttccccattg ctgacatccg ctttgaccgt cgtgcgcacg attccgccag ccaaagccga
     4741 cgccgacaag ctcgcggcgg cgctcaccaa gctggccgat agcgacggat ctgccgagat
     4801 tcgcctgacg gccctggtgg ccgtgccggg aggattgaaa tcggtttcgc cgagccagct
     4861 ggagttgctg ctcgctcata tcgaccgcga tcaagcgccc acgctccgcg gcctggcggc
     4921 ggaggcgatt tcgaaggcca agctcaccgg cgagcaactg catcagctgg tggataagct
     4981 gccgcgggca gcgccgatgg aactggatcg gctgctgacg ccgttcgctc agtccacc
//
LOCUS       DASRJB010000144         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3707821, whole genome shotgun sequence.
ACCESSION   DASRJB010000144 DASRJB010000000
VERSION     DASRJB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707821"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2189)
                     /locus_tag="VFB80_04090"
     CDS             complement(<1..>2189)
                     /locus_tag="VFB80_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN32969.1"
                     /translation="SDGRQVYVIDELGYAAVQNVTVNQVLVFGRGQRVPNNGWTKSHN
                     LLVALDLARQGYQVWAVGGTTADNPALAGAFFLGPPLPAGDQLFALAEFSGEIRLLCL
                     DPRTGGLLWKQQLAVLDEGQHIVWDSRRRLAGSSPSLAEGILICPTSAGALIAVDLAT
                     RTLRWGYQYGRSDITVTSSRVIRTTLNNEGTRWLDATATIADGCVVLTPVEEQRLHCL
                     DLLTGKAKWPPQARDDMLFVACVHDGKIVLVGQKRMKAISLADGKSAWPSPLELGGES
                     IVGRGYYSDHFYYLPVTGQQLLKIDLDTGKVEAKAQTEVELGNLVCYKDQLISQSPQT
                     VASFVLHSESLQKQLDERLAKNPQDIEALLLRAQILLQEGKANDSLKLLREAYKIAPE
                     KPTVRGLLAKVMLVMLRQDFAANLELTEELDKLVTDPAQRREVLRFRVQGLARTNRTW
                     EALAALLELADQELESAADRATVAGELQTVDRDLSTRSDRWLQGQMRTLLRGADSTIK
                     DRLSGEIKLRLERAIQSGSASQLRALLNLFGFDDSSTTARLALAEKLIAADALLEAEM
                     RLGEVLESGAGEHAGTVRAALAALYEKARRPELAARWYQELAASYAGVVCRDGLTGKQ
                     LAERAAANSAVQLHLAHAWPHGQVEVKDADANTNFDRLATFQRQQAYPVPMTRFAGAA
                     PRGLRVTYDLNQYLVSIRSDLGQTLATTSLRNTDGTAARRYYPHQYSL"
BASE COUNT          333 a          740 c          734 g          382 t
ORIGIN      
        1 agcgagtatt gatgtggata atagcgccgc gcggccgtgc cgtcggtgtt gcggagcgac
       61 gtggtggcca gggtctggcc caggtcgctg cggatcgaga ccaggtattg attgaggtcg
      121 taagtgacgc gcagcccgcg cggagcagcc ccggcaaacc gcgtcatcgg caccggatag
      181 gcctgctgcc gctgaaacgt cgccaggcgg tcaaaattcg tgttggcgtc ggcgtctttc
      241 acttcgacct ggccgtgcgg ccaggcgtgc gccaggtgca gctgcacggc agaattcgcc
      301 gccgcccgct cggccagctg ctttcccgtc aagccgtccc ggcagacgac gccggcgtag
      361 ctggccgcca gttcctgata ccaccgcgcc gctaattccg gccggcgagc tttctcatag
      421 agcgcagcca gcgccgcgcg gacggttccc gcgtgctcgc ccgcgccgct ttccaggact
      481 tcgcccagac gcatctcggc ctccagcagc gcgtcggcgg cgatcagctt ctccgcgagg
      541 gccaggcggg cggtcgtgct gctgtcgtcg aatccgaaca ggttcagcag cgcccgcagc
      601 tggctggcgc tgcccgactg aatcgcccgt tccaggcgca gcttgatctc gcccgacagc
      661 cgatctttga tcgttgaatc ggcgccgcgg aggagcgttc gcatctgccc ctgcagccag
      721 cggtcggacc tcgtggacag atcgcgatcg accgtctgca gctcgccggc caccgtcgcg
      781 cggtccgcgg ccgactccag ctcctgatcg gccagctcga gcagcgcggc cagggcttcc
      841 cacgtgcggt tggtgcgggc cagcccttgc acgcggaacc gcagcacctc gcggcgctgc
      901 gccggatcgg tgaccagctt gtccagctcc tcggtcaatt ccaggttggc cgcaaagtcc
      961 tgccgcagca tcacgagcat caccttcgcc aggagtccgc ggaccgtcgg cttttcggga
     1021 gcgatcttgt aggcctcgcg gagcagcttc agcgagtcgt tcgccttgcc ttcctggagc
     1081 aggatttggg cccgcaagag cagggcctcg atgtcctgcg gattcttcgc caggcgctcg
     1141 tcgagctgct tctgcagcga ttcgctgtgc agcacgaatg aagccacggt ctgcggcgac
     1201 tgggagatga gctggtcctt gtagcagacg aggttgccca gctcgacttc cgtctgggcc
     1261 ttggcttcga ccttgccggt gtcgagatcg atcttcagca gctgctggcc ggtgaccggc
     1321 aggtaataga aatggtcgct gtagtagccg cggccgacga tcgactcgcc gccgagctcc
     1381 agcgggctcg gccaggccga cttgccgtcg gccaggctga tcgccttcat ccgcttctga
     1441 ccgacgagga cgatcttgcc gtcgtggacg caggcgacaa acagcatgtc atcgcgagct
     1501 tgcggcggcc acttggcctt gcccgtgagc aggtccaggc agtgcagccg ctgttcttcg
     1561 acgggcgtga gcaccacgca gccgtcggcg atggtggctg tggcgtcgag ccagcgcgtg
     1621 ccttcgttgt tgagcgtcgt gcgaatgacg cgcgacgatg tgacggtgat gtcggagcgg
     1681 ccgtattgat agccccagcg cagcgtccgc gtcgccagat cgacggcgat cagggcgccg
     1741 gccgacgtcg ggcaaatcag gatgccctcg gccagcgacg gcgacgaacc ggccagccgc
     1801 cgccggctgt cccaaacgat gtgctggcct tcgtccagca cggccagttg ctgcttccag
     1861 agcaggccgc ccgtgcgcgg gtcgaggcaa agcagccgga tttcgccgct gaattccgcc
     1921 agggcgaaca gctggtcgcc ggccggcagc ggcggcccca ggaaaaatgc gccggccagc
     1981 gctgggttgt cggcggtcgt gccaccgacg gcccaaacct ggtagccttg ccgcgccaaa
     2041 tccagggcca cgagcagatt gtgcgacttg gtccagccgt tgttggggac gcgctgtccg
     2101 cggccaaaga ccagcacctg gttgaccgtc acgttctgca ccgcggcgta gcccagctcg
     2161 tcgatgacat aaacctgccg cccgtcgct
//
LOCUS       DASRJB010000145        23774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3424690, whole genome shotgun sequence.
ACCESSION   DASRJB010000145 DASRJB010000000
VERSION     DASRJB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 23774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23774
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424690"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5525
                     /locus_tag="VFB80_04095"
     CDS             <1..5525
                     /locus_tag="VFB80_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZN32970.1"
                     /translation="VVVEHDEETMWAADQIIDFGPGPGIRGGQLVVQGPPGEVIEHAG
                     SLTGKYLSGEKQIEIPKTRRPGSGNMLRILGATHNNLKNIDVEIPLGKFVCVTGVSGS
                     GKSSLVSDIIVETLRRDLNKGEGTPGAHRAIEGLEHLDKLIAIDQTPIGRTPRSNPGT
                     YIKVFDEIRDLFCQLPEAKRRGYKPGRFSFNVEGGRCEACEGNGSTKLEMDFLADVWV
                     TCPVCEGKRFSRETLQVKFKDKSIADVLEMDIQQLLVLFENVPHVRHKLQTLHDVGLD
                     YIKLGQPSPTLSGGEAQRIKLARELVKKSTGKTLYLLDEPTTGLHFADIQLLLKVLHD
                     FAIAGNTVLVVEHNLDVIKTADWVIDLGPEGGEGGGEVVFCGTPEELVAFSAGRGRRD
                     GEKERRREGVAARNHETPVSPSLLPSVSPSLPLRCHTGESLAPVLDGTYAAAMREAAR
                     KLNGKAGGKPRRGGQAELATHIVVRGARQHNLKSVDARIERDKMTIFCGKSGSGKSSL
                     AMDTIYAEGQRRYVESLSAYARQFVAQMQKPAVDSLDGLSPAIAIEQKNLGHTPRSTV
                     GTVTEIYDYLRILMARLGQLHCPQCEIPVGTQTVDQVVDSLLALPAGQRLYLMAPVEI
                     DVGQQYTALWEQLRATGYTRVRIDGETHSLDQAPQIDRRRKHKVEVVIDRVVAKPDAR
                     SRIAGTVENALALGKGVMHVAISQENVPESRWETIVHNQHLACKQCGRSFEPLTPHSF
                     SFNSPLGWCKTCEGLGVQIGANPAALLRDPKLSLPEGALELWPNLEQPLARAMLEALC
                     RGAGIPLDLPFERLAAKHRRMILHGTGEQWFEVGHNLGLGIGDLGLERKNTRSKIPNP
                     KSPIPNSPTFRFQFKGLYPAIEEASRLAPSLRVKLEHLVGEVDCSSCDGSRLRDDAAA
                     SRFRGRTIGELGKLPLGELARAIGGWNLTKTEQKIAGELLREITGRLQFLLDVGLDYL
                     SLSRPAASLSNGEAQRIRLASQLGSGLVGVLYVLDEPTIGLHPRDNTRLLRALQKLRD
                     LGNTLVVVEHDREVIQNADHVLDFGPGAGRLGGQIVADATPDKLGKAAGSVTGPYLSG
                     KKAIPVPTNRRISDLRFQISDLKNGKRKAAPKSEIRNLKSEMAPRPMLEVLGARHNNL
                     KNINVAIPLGTLTAITGPSGSGKSTLVDDILYSAIARKLHRASLVPGKHDAIRGLEHI
                     NKVIRVDQQPLGNSPTSNPATYTGAFELIRTLFSQLPEAKLRGYSARRFSFNVPGGRC
                     EACEGNGQRRIEMHFLPDVWVECETCRGKRYNPETLAVTFHGRSISDVLDMTCGDAVQ
                     LFDNIPKIRRILQTLCDVGLDYLTLGQPAPTLSGGEAQRVKLAAELSRPDTGRTLYLL
                     DEPTTGLHFDDLARLLEVLQRLVDIGNTVVIIEHNLDIIKSCDWVIDLGPEAGEAGGN
                     VVAAGTPEMVAERASETGRSGGEKERRREKAKAVAPSLRPSVSPSLPLRSHTAEALAP
                     TLAAGPFVERKPFNPAEQFAKREGDLEITEVGKTAKMPWEVDGRAWHTANRVGRTGEP
                     CRWDGRILAQIVDRIQELGEFSETDWSERSVVEICAPRKTDGWFFHAITGETWLLKLK
                     FRVYRGTFQRDELQQRIELKTLNELADLPIYGNEPRVKVKTLRGPWQEVEIRVHSLEE
                     IDTTEFWSFLDEAIAGFVKFAEKAAVNPEDISPWKVLGQKWHFLRKGFPPGKTVAWSV
                     ELLEELCELLRAAEPQGQFLWSNQQLVHFMVPQQREPWATIQTKKPSALLLHLIGPKG
                     RFGLGRLLELGADRDLDDTRDGHDIVRLQFVEEEHLHRGDLEKFLAEHLASLGKGN"
     gene            5609..6262
                     /locus_tag="VFB80_04100"
     CDS             5609..6262
                     /locus_tag="VFB80_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HZN32971.1"
                     /translation="MSSALISPALLFSFAAPCRYADEKWTPQGIQLGEQYRLPCFAEL
                     SGTKSFADIRLGWNEAGLAVNVAVRGKEQPPWCRDSRIEDSDGVQLFIDTRNTQNIHR
                     AGRFCHRFALMPIGAGQRLDEAVAVLLAINRAKESPREIDPRTLKVKSERLRDGYRLE
                     AFLPAAALTGYSPAEQPAIGFSYAVIDRELGRQTFSVANDLPFVEDPSLWGTLELVR"
     gene            6396..7622
                     /locus_tag="VFB80_04105"
     CDS             6396..7622
                     /locus_tag="VFB80_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32972.1"
                     /translation="MCCLVTSFAAAALAEDEPLPAPRGGLPAHRIVVRFSDALFNSLM
                     AGGIDRETTVHDNILGTSVYGRARVVAQPAVKLADCPDQATFQLVGEGTAYSRSTGYN
                     RQAVIYSHTVTSFRATKQVTFDPGRGFYSDPPQVVARAHTTIDGTGSLRGGIIGQIVR
                     RRAAREAAALRPQAEEIARQKAELRIAAAIDRYVEPRLARLNRAAEIRALVVSVLRPL
                     GSPEPNYACCSTPQHLQIATHFGEGGPAIELPVKGPASQAAAPIEVWVHRSLIGEPLA
                     ATLELLNDKARVSDLLVAARSAARNVDRRSSDGQWGDLWPMEWPVKLVQMRDWFVVEL
                     DYRADDRLDIARAPVARAPVAQPTANSLSPPGRGRQPPEPRRPAQTTENSLPPWHRPV
                     GGDIAAGSIPAARISR"
     gene            7695..8306
                     /locus_tag="VFB80_04110"
     CDS             7695..8306
                     /locus_tag="VFB80_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32973.1"
                     /translation="MSVAEPPPAGGQVNFPLDLAMIDPESSEKKPPYLVMFETGVDQN
                     DKDVRGPLGRLEALRGPSKMFPESNKDDGLPVVLRGLRFHRLLRSDGTLEGYEVELQG
                     EFNMVKVPVNAGEMKKFLSGERVSFSLTGRANYGVYAYASAIKMEVQLRGAELFIYKV
                     DGDFSFREGFTTYTSKTKKLSPPAGRDYIYRGETGQLPALPSI"
     gene            complement(8316..9644)
                     /locus_tag="VFB80_04115"
     CDS             complement(8316..9644)
                     /locus_tag="VFB80_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HZN32974.1"
                     /translation="MLWSVAIALLVLGLLAAGAVGLLLRGGGAERWLGLVPIRRDGRS
                     AVLFYAASHGELLLLKQLLPRLARERPDVESIIIVPRPSVLRLARDRLPHYRSHCVTP
                     LSPLAHGRVLRRLAAELFVIVEFARIPLWARAARRLGVPLTIVNGRINQRNLRACRRW
                     PWLFRPLLYGCGRILVQTAADAERFRQQGAQAAAIEITGAMKYEAAVGDRQARATRRL
                     AALARIAPGDVVLLGGSTTGHEEETLAEVFEQLAPTYPQLRLILVPKNRRRFASVAQM
                     LERRGLKFQRRSELETAGAERAARVLLVDTVGELAWWWGVARIGFVGASLVPRGGHNM
                     IEPAALGVATCFGPHTDDFRDVVAALSSYDAAVPVANSAELRTFVRRCLEEPAFAASL
                     GQRAQIVCRSQTGATLQTCQQLVQFLDQQHGQAVLTLPAAAAPSGRLRAA"
     gene            9871..10593
                     /locus_tag="VFB80_04120"
     CDS             9871..10593
                     /locus_tag="VFB80_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZN32975.1"
                     /translation="MRQRTSPFDPYLPQVGCWFVVLALAAVCLMPLVMLRVMEDALRH
                     LHLPGWLATLTVFGIFIGSLINLPLLRIDRGAEQPVMRGYMVPGLGWVPLPRKSEQTI
                     LAVNVGGCIIPVVLALFEASYVIYDMPPARWALAVAAGVNIAVCYFLARPIPGVGIAI
                     PAFVPPLAAIGLAWLLLSGEQFNEIRAPVAFVAGVAGPLIGADLLHLRRFADLRTGIL
                     SIGGAGTFDGIVISGILAAFFT"
     gene            10842..11909
                     /locus_tag="VFB80_04125"
     CDS             10842..11909
                     /locus_tag="VFB80_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN32976.1"
                     /translation="MSAVSRVARGLVGFALASHLAWAAAQEPPTLAKQNRWVTSAAFV
                     EGGARLVSGGGESLLYRAGDLKIWDAKSGALAASLEGQPTVVWSVAASADGKTLITSG
                     YDGKIILWNVAEKKPQQTIEKKGWIRRVALSPDGKHFAAAGEDGSVILFETDGGKEKK
                     TFKAHDSAVYDVAFSPDGAVLATCSTDKLAKLWDWNAETPAEKAKLEGHGDAVWSIAW
                     AKDGSLATASADRKIKLWNAEGKEQATLDGHKDWISSVTFSPDGNTLASCSHDRTVRL
                     WDVKEKKETASLGPHGSTCWSVAFSPDGALLAVGTHGQGVRLWNLGSKAEVFPAPAEK
                     KPEEAKPEAKKAEEKKPEEKK"
     gene            11959..12438
                     /locus_tag="VFB80_04130"
     CDS             11959..12438
                     /locus_tag="VFB80_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32977.1"
                     /translation="MLRHSIAMVGTFLALPLVGCRGEGGTMNDFSTPEKAIVSLENAY
                     RAGDLEAAIRCKDFKIEARLMLESISKLPKDQIDDELVGKTAEVLELGYRTEIKGKGL
                     PDMRGVRSTFPKQEPGGKNIVIVTEVCHYPDGGTSSQKILVAKTEQGWRVLNPVNDR"
     gene            complement(12462..13169)
                     /locus_tag="VFB80_04135"
     CDS             complement(12462..13169)
                     /locus_tag="VFB80_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32978.1"
                     /translation="MKTWPIVLLAVLLVSSPALAQRDSPVFSSYLPSIAVFDERGTLD
                     LDLLFCKAGGPIEHTEHQMYVLGYLEKDEAKILELAADKKLTSKKQEGGKLLMDLLEE
                     QKLATILDTKVAKREVVPPDQQDARRPGKGGHGFKYAFSLDNRSLFKAIGGLKNFKVD
                     MEMGRRSYNDKFKLLIFVPVNDCKYATKVPKELRDMYDFAHFYDSDTILLYFKPLPYR
                     FQFKPPDEQGAVELYVD"
     gene            13295..14626
                     /locus_tag="VFB80_04140"
     CDS             13295..14626
                     /locus_tag="VFB80_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN32979.1"
                     /translation="MRNLAAIGCLFLWACAGPASLPAEDLERAFRAARPGIVQQMKDR
                     DAETRVAAVRKLREFPIVEAARLILLQGCTSQHADVQRESFQTLVAISPQAEVRAHLQ
                     AEITREAKRGGSEFRSAAIACVLLCSDDAAAQASVKPWLEQMLKEKRGPALLVAMVDE
                     LAALRDEASLRALVQLGELPLIQKQFGPRRAWIQALIQVRQKDAVTLLLRRLPALHGE
                     TRSDILRHLTSLKGGRLDTDDQWLAWWMEQQATFKFPAAGDAPAQIAARQGEASYYGL
                     PLYGTKIVFILDTSGSMSGPRILAAQRELVSAIAALPEGVEFNVLAFNSAVAVWQSQL
                     VPASAATKEAAARFVMAQVAREQTASFDALDAAFVFDAEAIYFLTDGAPTTGKIKQPD
                     QIVAALSGPNRVRRITVNCLGIGVGQAGGPFDKFLNSLATQNFGLYRRVDQ"
     gene            14661..15740
                     /locus_tag="VFB80_04145"
     CDS             14661..15740
                     /locus_tag="VFB80_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN32980.1"
                     /translation="MRVTSVSVTVVEVPQTAPLAPYRSHLRSSSTTQSAIARVETDAG
                     LVGWGEHNINFLPNLSGRQMTRAAQWLVGRDARNISRFHQECPLETRLKSGLELALWD
                     ICGQAAGLPVAALLGGVIRERVEVAACMGIQSYERAKEMAAWYVEQGFGTLKTKAGSD
                     MREDLEMVRGVRDAVGDRLKLRIDPNRAYSPQQAAELGRQLEQYSLEYFEQPIPAEPL
                     ADAAWLRRQTRTPIALNESVTDPASVLAILRADAAAFLLPDTHIAGGIWPCVAIGQLC
                     AAAGIPCIMHCGHDLGPKTAAMLHVAAACPAYTLANDTTYYGLEDDIITERLSIERGT
                     MAVPARPGLGVAGDPERIARYAIDC"
     gene            15763..17112
                     /locus_tag="VFB80_04150"
     CDS             15763..17112
                     /locus_tag="VFB80_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN32981.1"
                     /translation="MQPADKPTNVRWVVFSLACGTSWLLYLHRYSFALFKPELVKQWE
                     LSKTELGLLDGAFSIGSIGFQIPFGVAADAVGVRLVLTCLLVLGSVGLGMHAWAPSPK
                     YLWPARLVFGIGQSAVYACLSRIAQTWFPPRIRTALQSTAAITFGRLGGLTANLILGG
                     VVLGLYGVDWRVATNVMAAGGVIFAIAVALVFRNSPREQPLANEAEADLIDPPQAATA
                     DTPPRMSVSQMLRSLRPAAVPNFVWLNVQTILSTFADNIYSHWIPLFLWEVHQLKFGP
                     LGLYSSLPLLGGAIAGILGGMLNDGCIAASGNRRWSRRVVAICGKGLAAVLLLAALAW
                     YHNPYVFCWFLFAVKFFGDWSLATSWGVVTDIGGRATASVFAVNNTVAGIGLFAAPIV
                     FGFVADHWGWPAVFVAVAVAYILCALSWLGIDSTQPLLDDRKPLSPGPQAGQIGQVG"
     gene            17270..18340
                     /locus_tag="VFB80_04155"
     CDS             17270..18340
                     /locus_tag="VFB80_04155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZN32982.1"
                     /translation="MKVYAEPGRFGGWPANHGVWSWGDEILVGFSAGYAKDNGPGRHA
                     IDHDRPEEHLLARSRDGGATWSIENPAKTGALIPTGAALHGVTPPGLKERPWEDCPGG
                     IDFTHPDFALTVRMTDVNAGSSRFYYSTDRGHAWLGPFRLPLFEQPGIAGRTDYVVLG
                     KHDCLLMLTAAKRNGREGRPLAVRTTDGGRSWQRLGWLGDEPAGYAIMPSTVLLGSAR
                     LGDARLGENELLSAVRCREGARSWIDLYRSRDLGRSWLLAGAAVAETGEGNPPSLIRL
                     QGGRLCLTYGYRAEPFSIRARISSDGKTWSPERTIRGAGGGRDIGYPRSVQRPDGKVV
                     TIYYFHDQPASDRYIAATIWTP"
     gene            18626..19333
                     /locus_tag="VFB80_04160"
     CDS             18626..19333
                     /locus_tag="VFB80_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32983.1"
                     /translation="MRAENRWPDERQAGPFLCHADFALAPQQPLLDELSALRQDLQAI
                     LGTPESADPVHLFLFQEKPTYQAYLKQHFPRVPYRRALFIKGRGPGMVFAFQGTDFQI
                     DVRHECTHALLNAWLPRVPLWLDEGLAEYFEVERDKRSAGNPHHQVVRAMFQSGQRPR
                     LEELEAIETLDAMGRDEYRDAWAWVHFMLHGPRPAHDELVRYLGELESGGESGRLSDR
                     LKRRLPDLDRRLDEHFR"
     gene            complement(19385..19855)
                     /gene="ruvX"
                     /locus_tag="VFB80_04165"
     CDS             complement(19385..19855)
                     /gene="ruvX"
                     /locus_tag="VFB80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZN32984.1"
                     /translation="MSDARPDDLPRTGRLAGIDFGTVRIGVAITDPDQRLASPHENYT
                     RNGLVADAAWIKRLAASERVVGFVVGLPVHGSGEESQKSREAGQFAAWLAKETKLPVR
                     LFDERYTTAHAEAALLEAGLTKKRRRERLDKLAAQILLAAYLESSRSDAEPGAL"
     gene            complement(19852..20841)
                     /locus_tag="VFB80_04170"
     CDS             complement(19852..20841)
                     /locus_tag="VFB80_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HZN32985.1"
                     /translation="MRAHLFVFLAFAPAFAAPLRGEDPPPVNVPLPTLGGEQFWSDEL
                     VYQGWRIQQNVLTQHYRLLDPRDVRRAWGTAEQCRAAFAEFKRHENLPPLSGRAVVTL
                     HGLIRSRDHMDGLGKFLAEEGKYHVVNVSYASTRRSLDDHALSLSRVLAGLEGVEEID
                     FVGHSLGNLVVRRYLGEASQSEPRWQVDPRIKRMVMLGPPNNGARLAVLFRNNELFGL
                     VTGPSGKQLARSWDECQKTLATPSFPFGILAGGSGNDRGLNPLVPGDDDLIVSVEETR
                     LAGAADFRLLHCRHALLMDDPAARACVLAFLQHGYFTAEGERQPIVVAAKAAP"
     gene            complement(21618..22175)
                     /locus_tag="VFB80_04175"
     CDS             complement(21618..22175)
                     /locus_tag="VFB80_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32986.1"
                     /translation="MSVGTLLRSLYLLHFSQPASDRAVYRAVKSRPIRSIVEIGIGSG
                     GRTQRLLEVAAWRAASLPLRYTGIDLFEARPAGSPGHSLKEAFALFQRPGVKVQLVPG
                     EADVALRRVANALAGTELLLISAGLDAESLARAWTWLPRMLTAQSSVFLEEPAKAGAT
                     GWRKLTIADVQQRAGAASRALRRAA"
     gene            22548..>23774
                     /locus_tag="VFB80_04180"
     CDS             22548..>23774
                     /locus_tag="VFB80_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN32987.1"
                     /translation="MQRQGEAERERGWKFETFEERLALSAEPLADFWYDLDPETLSEP
                     SSAVVQPLAAEGHGWTDVAGVREQFGLRGGDQTVAIIDSGIAYDHIALGGGLGKAYRV
                     VGGWDFAENDANPYDDGPAGFHGTHVAGIVGAMDGKYPGVAPGVDLVALRVFNDQGGG
                     NFAWVEQALQWVHTNRTAFDNPITTVNISLGTEWNSAALPQWATLEEELAQLARDGIF
                     ISVAAGNSFLAYGTPGLSYPAVSQNVTPVASVDGSGNLSRYTQRSERVLAAPGERIMS
                     TLPDHFYGGDGVKNDWGATSGSSMAAPYVAGASVLVREAMQDLGVTNITQGTIYDLLR
                     RTADTIYDAATSASYKRVNLTRALETLVGVDDYGSTAEAATSLGRLATSQRVSGTIGR
                     VGDEDFFQITAGSTGQL"
BASE COUNT         4138 a         7863 c         7894 g         3879 t
ORIGIN      
        1 ttgtcgtcgt cgagcacgac gaagagacga tgtgggccgc ggaccagatc atcgacttcg
       61 gccccggtcc gggcatccgc ggcggccagc tcgtcgtgca aggcccgccc ggcgaggtca
      121 tcgagcacgc cggcagcctc accggcaagt acctctcggg cgagaagcag atcgagatcc
      181 cgaaaacccg cagaccgggc agcggcaaca tgctgcgcat tctcggcgcg acgcacaaca
      241 acctgaagaa catcgacgtc gagatcccgc tcggcaaatt cgtctgcgtc acgggcgtca
      301 gcggcagcgg caagagctcg ctggtcagtg acatcatcgt cgagactctg cgtcgcgacc
      361 tgaacaaggg cgagggcacg cccggcgctc accgggccat cgaagggctg gagcatctcg
      421 ataagctgat cgccatcgac cagacgccga tcggccgcac gccgcgctcg aaccccggca
      481 cctacatcaa ggtcttcgac gaaatccgcg acctgttctg ccaattgccc gaggccaagc
      541 gccgcggcta caagcccggc cgcttcagct tcaacgtcga agggggccgc tgcgaagcct
      601 gcgaaggcaa cggcagcacg aagctggaaa tggatttcct cgccgacgtg tgggtgacat
      661 gtccggtgtg cgaaggcaaa cggttcagcc gcgagacgct gcaggtcaaa ttcaaagaca
      721 agtcgattgc cgatgtgctg gagatggaca tccagcagct gctcgttctg ttcgagaacg
      781 tgccgcacgt gcggcacaag ctgcaaacgc tgcacgacgt cggcctcgat tacatcaagc
      841 tcggccagcc ctccccgacg ctctccggcg gcgaggccca gcggatcaag ctggcccgcg
      901 agctggtgaa gaagagcacc ggcaagacgc tctacctgct cgacgagccg acgaccggcc
      961 tgcacttcgc cgacatccag ctgctgctca aagtcctgca cgacttcgcc atagccggca
     1021 acaccgtcct cgtcgtcgag cacaacctcg acgtgatcaa gacggccgac tgggtaatcg
     1081 atcttggtcc ggagggcggc gagggcggcg gggaggtggt gttttgtggg acgccggagg
     1141 agttggtggc gttttctgcg ggtcggggaa ggagagacgg agagaaagag cgacggagag
     1201 aaggagtggc ggcgagaaac cacgaaacac cagtctctcc atccctcctt ccctccgtct
     1261 ctccgtctct ccctctccgc tgccacaccg gcgagtcgct ggcaccggtg ctggacggca
     1321 cgtacgccgc cgccatgcgc gaggcggcgc ggaagctgaa cggcaaggcc ggcggcaagc
     1381 cgcgccgcgg cgggcaggcc gagctggcca cgcacatcgt cgtccgcggc gccaggcaac
     1441 ataatctcaa gtcggtagat gcccgcatcg agcgggacaa gatgacgatc ttctgcggca
     1501 agtccggcag cggcaaatcg tcgttggcga tggacacgat ctatgccgaa ggccagcggc
     1561 ggtacgtcga atcgctctcg gcctacgccc ggcagttcgt cgcccagatg cagaagccgg
     1621 ccgtcgattc gctcgacggc ctgtcgccgg cgattgccat cgagcaaaag aacctcggcc
     1681 acacgccccg ctcgaccgtc ggcaccgtca cggaaatcta cgattacctc cgcatcttga
     1741 tggctcgcct gggccagctc cactgtccgc agtgcgagat cccggtcggc acgcagaccg
     1801 tcgatcaggt cgtcgatagc ctgctggccc tgccggccgg ccagcggctc tatctgatgg
     1861 ccccggtcga gatcgacgtc gggcagcagt acaccgccct ctgggagcag cttcgcgcaa
     1921 ccggctacac gcgcgtgcgg atcgacggcg agacgcactc gctcgaccag gcgccgcaga
     1981 tcgaccgccg ccgcaagcac aaggtggagg tggtcatcga ccgggtcgtc gccaagcccg
     2041 acgcccgctc gcgaatcgcc ggcaccgtcg aaaacgcgct ggccctgggc aagggcgtaa
     2101 tgcacgtcgc gatctcccag gaaaatgtgc ccgaaagccg ctgggagacg atcgtccaca
     2161 accagcatct ggcctgcaag cagtgcggcc gcagcttcga gccgctcacg ccccacagct
     2221 tctcgttcaa cagcccgctc ggctggtgca agacgtgcga aggcctcggc gtgcagatcg
     2281 gcgcgaaccc ggccgcgctg ctgcgcgatc ccaagctttc gctgcccgag ggcgcgctgg
     2341 agctctggcc caacctcgag cagccgctgg cccgcgcgat gctcgaagcg ctctgccgcg
     2401 gcgcgggaat cccgctcgat ctgcccttcg agcgcctggc cgccaagcac cgaaggatga
     2461 ttttgcatgg cacgggggag cagtggtttg aggtcgggca caatttggga ttggggattg
     2521 gggatttggg attggagaga aagaacaccc gctcaaaaat cccaaatccc aaatccccaa
     2581 tccccaatag cccgacattc cgcttccagt tcaaaggcct ctaccccgcc atcgaagaag
     2641 cttctcgcct tgcgccgagc ttgcgggtga agttggaaca tctggtgggc gaggtcgatt
     2701 gctcgtcgtg tgacggcagc cggctgcgcg acgatgcagc ggccagccgc tttcgtgggc
     2761 ggacgatcgg cgagctggga aagctgccgc tgggggaact ggcccgcgcg atcggcggct
     2821 ggaacctcac caagacggag caaaagattg ccggcgagct gctgcgcgag atcaccggcc
     2881 gcctgcaatt cctgctggac gtgggcctcg attatctctc gttgtcgcgg ccggcggcga
     2941 gcctttccaa cggcgaggcc cagcggattc gcctggccag ccagctcggc agcggcctgg
     3001 tcggcgtgct ttacgtgctc gacgagccga cgatcggact gcacccgcgc gacaacacgc
     3061 ggctcttgcg cgccctgcaa aaactccgcg acctgggcaa cacgctggtg gtcgtcgagc
     3121 acgaccgcga ggtgatccag aatgccgacc acgtgctcga cttcggcccc ggcgcgggcc
     3181 gcctcggcgg ccagatcgtc gccgacgcca cgccggacaa gctcggcaaa gctgccggca
     3241 gcgtcaccgg gccgtacctc agcggcaaga aggcgatccc cgtgccgacg aaccggagaa
     3301 tttcagattt gcgatttcag atttcagatt tgaagaacgg caaacgcaag gctgctccca
     3361 aatctgaaat ccgaaatctg aaatctgaaa tggccccgcg gcctatgctc gaagtcctcg
     3421 gcgcccggca caacaacctc aaaaacatca acgtcgccat cccgctcggc acgctgacgg
     3481 cgatcacggg accgagcggc agcgggaaga gcacgctggt cgatgacatc ctctattcgg
     3541 cgatcgcccg caagctgcac cgggcgtcgc tggtgcctgg caagcacgac gcgattcgcg
     3601 ggctggagca catcaacaag gtgatccgcg tcgatcagca gccgctgggc aactcgccga
     3661 cgagcaaccc ggcgacctac accggggcgt tcgagctgat ccgcacgctc ttctcgcagc
     3721 ttcccgaggc caagctccgc ggctattcgg cccggcggtt cagcttcaac gtgccgggcg
     3781 ggcggtgcga agcctgcgag gggaacggcc agcggcggat cgagatgcac ttcctgcccg
     3841 atgtgtgggt cgagtgcgag acctgccgcg gcaagcgcta taaccccgag acgctggcgg
     3901 tcacgttcca cggccgctcg atcagcgacg tgctcgacat gacctgcggc gacgccgtgc
     3961 agctgttcga caacatcccc aaaattcgcc gcatcctgca aacgttgtgc gacgtggggc
     4021 tcgactacct cacgctcggc cagcccgcgc cgacgctttc cggcggcgaa gcccagcggg
     4081 tgaagctcgc ggccgaattg tcgcggcccg acaccggccg cacgctctac ctcctcgacg
     4141 agccgacgac ggggttgcac ttcgacgacc tcgcccggct gctggaagtg cttcagcggc
     4201 tcgtggatat cggcaacacc gtcgtcatca tcgagcacaa cctcgacatc atcaaaagct
     4261 gcgactgggt gatcgacctg ggaccggagg cgggagaagc aggcgggaat gtggtggcgg
     4321 cgggaacgcc ggagatggtg gctgagcggg cgtcggagac aggacggagt ggcggagaga
     4381 aagagagacg gagagagaag gcaaaggcag tcgctccgtc cctccgtccc tctgtctctc
     4441 cgtcgctccc tctccgttca cacacggccg aggctcttgc gcccaccctc gccgccggcc
     4501 cgttcgttga gcgcaagccg ttcaacccgg ccgagcagtt tgccaaacgc gaaggcgacc
     4561 tggagatcac cgaggtcggc aagaccgcga aaatgccgtg ggaggtggat gggcgggcgt
     4621 ggcatacagc aaaccgcgtg ggccgcaccg gcgagccctg ccgctgggac ggccgcattc
     4681 tggcccagat cgtcgatcgg attcaggagc tgggcgaatt ctccgaaacc gattggagcg
     4741 aacggagcgt cgtcgaaatt tgcgctccca ggaagaccga cggctggttc ttccacgcga
     4801 ttaccggcga gacctggctg ctgaagctca agttccgcgt ctatcgcggc acgttccagc
     4861 gcgacgaatt gcagcagcgg atcgagctca agacgctcaa cgagctggcc gacctgccga
     4921 tctatggcaa cgagccgcgg gtgaaggtga agacgctccg cgggccgtgg caggaagtcg
     4981 aaatccgcgt ccactcgctg gaggagatcg acacgaccga gttctggtcg ttcctggacg
     5041 aagcgattgc cggcttcgtg aagttcgccg agaaggcggc cgtcaacccg gaggacattt
     5101 cgccctggaa ggtgctcggc cagaagtggc acttcctccg caaaggcttt ccgcccggca
     5161 agacggtcgc ctggagtgtc gaattgctgg aagaactgtg cgagctgctc cgggccgccg
     5221 agccccaggg acagttcctc tggagcaacc agcaactcgt ccatttcatg gtgccgcagc
     5281 agcgcgagcc ctgggccacg atccagacga aaaagccgag cgccctgctc ctgcacctga
     5341 tcggccccaa aggccggttc ggcctcggcc gcctgctgga gctcggcgcc gaccgcgacc
     5401 tcgacgacac ccgcgacggc cacgacatcg tgcggctgca attcgtcgag gaggagcacc
     5461 tgcatcgcgg cgacctggag aagtttctgg ccgaacacct ggcgtcgctg gggaagggga
     5521 attgagggtc taggaggcaa gttcaaggtt caaagttgga tcgcgacatc gaatgcccaa
     5581 aatctgaaat ctgaaatccc aaatcgaaat gtcttccgcc ctcatctctc ccgcgctcct
     5641 attcagcttc gccgcgccgt gccggtatgc ggacgaaaag tggacccccc agggaattca
     5701 gctgggcgag caataccggc tgccttgctt cgccgagctg agcgggacga agtcgtttgc
     5761 cgacatccgg ctgggctgga atgaggcggg gctcgcggtg aacgtggcgg tgcgcggcaa
     5821 agagcagcct ccttggtgcc gcgattcgcg gatcgaggac agcgatgggg tgcagctctt
     5881 catcgacacc cgcaacacgc agaacatcca ccgtgcgggc cgcttctgcc accgcttcgc
     5941 gctgatgccg atcggcgcgg gccagcggct ggatgaggcg gtggcagtcc tgctggccat
     6001 caaccgcgcc aaggaaagcc cccgtgagat cgatccccgc acgctcaagg tgaagagcga
     6061 gcggctgcgg gatggctacc gcctggaggc gtttctgccg gcggccgccc tcacgggcta
     6121 cagtccggcc gagcagccgg cgatcgggtt ctcgtacgcg gtgattgacc gcgagctggg
     6181 ccggcagacg ttcagcgtcg ctaacgacct gccgtttgtc gaagatccca gcctgtgggg
     6241 gactctggag ctggtacgtt agcgcgccaa agcgggcgtt gctcgggctc cggcacggaa
     6301 cctgcaccct ccgtgtgcgt tttactgaac tctcacgggt gaggcgggcc atgaacgggc
     6361 aacgtcatgc caggcgccgc gcctggactt acgcagtgtg ctgcctcgtc acctcgttcg
     6421 cagcggccgc gctggcggag gacgaaccac ttcccgcccc gaggggcggt ctgccggcgc
     6481 atcgcatcgt cgtacgtttc tccgacgcgc tgttcaactc gctgatggcc ggcggcatcg
     6541 atcgcgagac tacggttcac gacaacattc tgggcacatc ggtctatggg cgggcgcggg
     6601 tcgttgctca gccggcggtc aagttggcgg actgtccgga ccaggcgaca tttcagctag
     6661 tcggggaggg gacggcctat tcgcgctcca caggctacaa ccgccaggcc gtcatttaca
     6721 gccacacggt gactagcttc agggccacta agcaggtcac gttcgacccc ggccggggct
     6781 tttactcgga cccgccgcaa gtcgtggccc gcgctcacac aacgatcgac ggcaccggct
     6841 ccttgcgcgg cgggatcatc gggcaaatcg tgcggcggcg agccgcgcgc gaggctgccg
     6901 ccctgcggcc ccaggccgag gagatcgcgc ggcaaaaggc ggagctgcga attgccgccg
     6961 ccatcgaccg ctacgtcgag ccgcggctgg cccggctcaa ccgggcggcg gaaattcgcg
     7021 cgctggtcgt gtcggtgctg cgccccttgg ggagcccgga accgaattac gcctgctgtt
     7081 ccacgccgca gcacctgcaa atcgcgacgc acttcggcga gggcggcccg gcgatcgagc
     7141 tgccggtcaa gggcccggcc agccaggcgg cggccccgat cgaagtctgg gttcaccgct
     7201 ccctgatcgg cgagccgctt gccgcgaccc tcgagctgct gaacgataag gcgcgagtga
     7261 gcgacttgct ggtcgccgcg cggtcggccg cgcggaacgt cgatcgccgc agcagcgatg
     7321 gccagtgggg tgacctgtgg ccgatggagt ggccggtcaa gctggtccag atgagggact
     7381 ggttcgtcgt cgagttggac taccgcgccg acgaccgact ggacattgcc cgagcccccg
     7441 tcgcccgagc ccccgtcgcg cagccgaccg caaactcgct ctctccgcct ggccgcgggc
     7501 gacaaccccc cgaaccgcgt cggccggcgc agacgaccga aaactcattg cctccctggc
     7561 accgcccagt gggcggcgat atcgctgcgg gcagcatccc cgccgcccgt atttcgagat
     7621 agaattgcgg catgaaacgg actttatgct tcgtttgcgt cgccagtgca ctctttctgg
     7681 tggcacgacc agcggtgtcg gttgctgaac ctccgcctgc cggcggccag gttaactttc
     7741 cgctcgatct ggcgatgatc gatccggaat cgtccgagaa gaagccccct tacctcgtga
     7801 tgttcgagac gggcgtcgat caaaacgaca aggacgtccg cggcccgctc ggccggctgg
     7861 aagccctccg cgggccctcg aagatgtttc ccgaaagcaa caaggacgac ggcctgcccg
     7921 tcgtgctccg cgggctgcgg ttccaccggc tgctccgctc cgatggcacg ctggaaggct
     7981 acgaggtcga gctgcagggg gaattcaata tggtcaaggt gccggtcaac gcgggagaga
     8041 tgaagaagtt cctgagcggc gagcgggtca gcttctcgct caccggccgg gccaattacg
     8101 gcgtttatgc ctacgcctcc gcgatcaaga tggaagtgca gctccgcggt gcggagctct
     8161 tcatctacaa agtggacggc gacttctcgt tccgcgaagg cttcacgacc tacaccagca
     8221 agacgaagaa gctcagcccg ccggccggcc gcgactatat ctaccgcggc gagaccggcc
     8281 agctgccggc gctgccctcg atctaagtct gggcgctacg ccgcgcggag ccgcccgctc
     8341 ggcgcggccg cagccggcag cgtgagaacc gcctgcccgt gctgctgatc gaggaactgg
     8401 acgagctgct ggcacgtttg cagggtcgcg cccgtttgtg agcggcagac gatttgcgcc
     8461 cgctggccca acgatgcggc gaaagccggc tcctcgaggc agcggcggac gaacgtccgc
     8521 agctcggcgg aattggcgac cggcacggcg gcgtcatagc tggacagcgc ggcgacaacg
     8581 tcgcgaaagt cgtcggtgtg cggcccaaaa caggtcgcca cgcccagcgc cgccggctcg
     8641 atcatgttgt ggccgccgcg cgggaccagg ctcgccccga cgaagccgat gcgggccact
     8701 ccccaccacc aggccaattc gcccacggta tcgaccagca agacgcgggc tgccctttcc
     8761 gcgccggccg tctcgagctc cgagcgccgc tgaaacttca gcccgcggcg ctcgagcatt
     8821 tgcgccacgc tggcgaatcg ccgccgattc ttgggcacca ggatcagccg caattgcgga
     8881 taggtcggcg cgagctgctc aaacacctcg gccagcgttt cctcttcgtg gccggtcgtg
     8941 ctgccgccga gcagcaccac gtcgcctggt gcgatcctgg ccagtgccgc cagtcggcga
     9001 gtggcccgcg cttggcgatc gccgaccgca gcctcgtact tcatcgcgcc cgtgatttcg
     9061 atggcggctg cctgggctcc ttgctgccgg aagcgttcgg cgtcggccgc cgtctgaacc
     9121 aggattcgcc cgcagccgta cagcaggggc cgaaacaacc acggccagcg gcggcaggcc
     9181 cgcaagtttc tctggttgat ccgcccgttc acgatggtca gcggcacgcc cagccgccgc
     9241 gccgcgcggg cccacagcgg aatgcgggcg aattcgacaa tgacgaagag ctccgccgcc
     9301 agccgccgca gcacgcggcc gtgcgccagg ggcgagagcg gcgtcacgca gtgcgaccgg
     9361 taatgcggca ggcggtcccg ggccaaacgc aggacgctcg gccgcggcac gatgatgatc
     9421 gactcaacgt cgggccgctc gcgcgccagc cgcggcagca gctgcttcag caagagcagt
     9481 tcgccgtggc tggcggcata gaacagcacg gcggacctgc cgtcgcgccg gatcggcacc
     9541 agaccgagcc agcgctcggc cccgccgccg cggaggagca gcccaacagc gccagcggca
     9601 agcaggccca ggaccaatag cgcgatggcg acggaccaca gcatggtgcg gaactgtcag
     9661 caaattcgcg attttgggca agcacagttt tctcaccgct gctagcagcg cctggtttga
     9721 ctttcccctg ccgcggcatc ccaatagtgg gcgactcgct ccgcgagtcg ccggagcggc
     9781 gtggctgcca cggacacggc agtgctccgg tcgattcata atagaaagcg gttttggtca
     9841 ctcgcggagc gagtggccca caatgacgcc atgcgccagc ggaccagccc cttcgatccc
     9901 tacctgccgc aggtcggctg ctggttcgtg gtgctggcgt tggcggcggt ctgcctgatg
     9961 ccgctagtca tgctgcgggt catggaagac gccctgcggc acctgcacct gccgggctgg
    10021 ctggcgacgc tgacggtctt cggaatcttc attggcagcc tgatcaatct gccgctcctg
    10081 cggatcgacc gcggcgctga gcagccggtg atgcgcggct acatggtgcc gggcctgggc
    10141 tgggtgccgc tgccgcggaa aagcgagcag acgatcctgg cggtgaacgt cggcggctgc
    10201 atcatcccgg tggtgctggc gctgttcgag gcgagctacg tcatctacga catgccgccg
    10261 gcccgctggg cgctggcagt ggcggcaggc gtgaacatcg ccgtctgcta tttccttgcc
    10321 cggccgattc cgggcgtagg cattgcgatc ccggcgttcg tgccgccgct ggcagcgatc
    10381 ggcctggcgt ggctgctcct gagcggcgag cagttcaacg agatccgcgc gccggtcgcc
    10441 ttcgtggccg gagtcgccgg gcctctgatc ggagccgacc tccttcacct gcgacggttc
    10501 gccgacctgc ggacgggcat tctctccatc ggcggcgcgg gcaccttcga cggcatcgtc
    10561 atctccggca tcctggccgc gttctttacc tagggagcat tgcagtggcc aaagccgcca
    10621 caaaatcggc cgtcgctgca tggctcgctc ctcggtcttg cgacaccaac ccgaagcgta
    10681 agcgagggac attctgagtt cgtcccgaat gaccctcgct tacgcttcgg gttacgattg
    10741 caccaattaa gacgttatgt cttagaagtc aaaccttgca ggcgcaggcg cgcagaacgt
    10801 agggtattac atcgccttga attccaccgg ggaggtcttt catgtccgcc gtttcgcgcg
    10861 tcgcccgtgg tttggttggt ttcgccctcg cttcccacct tgcttgggcc gcggcccagg
    10921 agccgccgac gctggcgaag cagaatcgct gggtcacgtc ggcggcgttc gtcgagggcg
    10981 gggccaggct cgtcagcggc ggcggcgaga gcctgctgta tcgcgcgggc gatttgaaga
    11041 tctgggacgc caagagcggc gctctggccg ccagcctgga ggggcagccg acggtcgtct
    11101 ggtcggtcgc ggcctcggcc gacggcaaga cgctgatcac cagcggctac gacggcaaga
    11161 tcattctctg gaacgtcgcc gagaaaaagc cgcagcagac gatcgagaag aagggttgga
    11221 ttcgccgggt ggccctttcg ccggacggca agcactttgc cgccgccggt gaggacggca
    11281 gcgtaatcct cttcgagacc gacggcggca aggagaagaa gactttcaag gcccacgact
    11341 cggccgtgta cgacgtggcg ttttcgcccg acggggcggt cctggcgacc tgctcgaccg
    11401 acaagctggc gaagctctgg gattggaatg ccgaaacgcc ggcggagaag gccaagctgg
    11461 agggacacgg cgatgcggtc tggtcgatcg cctgggcgaa agacggctcc ctggccaccg
    11521 ccagcgccga ccgcaagatc aagctctgga acgctgaggg caaggagcag gccacgctgg
    11581 atgggcacaa ggactggatc agcagtgtca cgttttcgcc cgacggcaac accctggcca
    11641 gctgcagcca cgaccgcacg gtccgcctct gggacgtcaa ggaaaagaag gaaaccgcgt
    11701 cgctagggcc ccacggcagc acctgctggt cggtcgcctt ctcgcccgac ggcgcgctct
    11761 tggccgtcgg cacgcacggg cagggcgtcc gcctctggaa cctcggcagc aaggccgaag
    11821 tctttcccgc cccagcagag aagaagccgg aagaggccaa accggaggcc aagaaggccg
    11881 aagagaaaaa gcccgaagag aagaagtagc gcgtgaggcg tgacttcctc aatccgaaag
    11941 gctacggcca agacactcat gctgcgtcat tcaatcgcca tggtcgggac attccttgcg
    12001 ttgcctcttg tcggatgccg aggcgagggc ggcacaatga atgatttctc aactcctgaa
    12061 aaggcaatag ttagcctgga gaacgcctat cgcgccggag atttagaggc ggcgatcaga
    12121 tgcaaggact tcaaaatcga agcgcgactg atgttggaga gcatctccaa gttgcccaaa
    12181 gatcagatcg acgacgaact ggtgggcaaa accgcagagg tgctggagct tggctaccgc
    12241 acggaaataa agggaaaggg attaccggac atgaggggcg tgcgttcgac atttccgaag
    12301 caggagccgg gcggcaagaa tatcgtgatc gtcacggagg tctgtcacta cccggacgga
    12361 ggtacgtcca gtcaaaaaat tctggtcgcg aagaccgagc agggctggcg agtacttaac
    12421 ccggtcaatg atcgctgagg ttacctctac cgctgcaggg ctcaatccac gtaaagctcc
    12481 accgcccctt gttcgtccgg cggcttgaat tgaaagcggt aggggagcgg cttgaaatag
    12541 agcaggatcg tgtccgagtc gtagaagtgg gcgaagtcgt acatgtcccg cagttccttg
    12601 gggaccttgg tcgcatactt gcagtcgttg acgggcacga agatcagcag cttgaatttg
    12661 tcgttgtagg agcggcggcc catctccata tcgactttga agttcttgag gccgccgatc
    12721 gccttgaaga gcgaacggtt gtccagactg aacgcgtact tgaagccatg gccccctttt
    12781 cctggccggc gcgcgtcttg ctggtccggc ggcacgactt cgcgcttggc cactttggtg
    12841 tcgagaatgg tcgccagctt ttgctcttcc aaaagatcca ttagcagctt tccgccctcc
    12901 tgcttcttgc tggtgagctt tttgtcggcc gccaactcca gaatcttggc ctcatctttc
    12961 tccaggtagc ccaggacata catctggtgc tcggtgtgtt cgattggccc gccggccttg
    13021 caaaacagca agtccaggtc gagcgtcccg cgctcatcga agaccgcgat gctcggcaga
    13081 taggagctga aaaccggcga atcccgttga gccaacgccg gggatgaaac caataacacc
    13141 gccagcagca cgatcggcca ggttttcatg gaggcaccgt aggttggagg cgcagtcgtt
    13201 ctatcacacg cggcgcggcc ggacaaatct ccgcccacgt cggatcatgg cgcatgacct
    13261 ggaggccagc gcgccgcgcg gtagaatccc gcgtgtgaga aatcttgctg ccatcggctg
    13321 cctgtttctg tgggcctgtg ccgggccggc gagcttgccc gccgaggacc tggagcgggc
    13381 gtttcgcgcg gcgcggccgg gaatcgtgca gcagatgaag gaccgcgacg ccgagacgcg
    13441 cgtcgctgcc gtccgtaagc tgcgggagtt tcccatcgtc gaagcggccc ggctgatcct
    13501 gctgcagggc tgcaccagcc agcacgccga cgtgcagcgc gagtcgtttc agacgctggt
    13561 cgccatcagc ccgcaggctg aagtgcgggc gcacctgcaa gccgagatca cccgcgaggc
    13621 aaaacgtggc ggatcggagt tccgctcggc ggccatcgcc tgcgtgctgt tgtgcagcga
    13681 cgatgcggcg gcgcaggcca gcgtcaagcc gtggctggaa cagatgctga aggagaagcg
    13741 cgggccggcc ctgctggtgg cgatggtcga tgagctggcg gccttgcgcg acgaagcgag
    13801 cctgcgggcg ctagtccagc tcggcgagct gccgctcatt caaaagcagt tcgggccgcg
    13861 gcgggcctgg attcaggccc tgatccaggt ccggcaaaag gacgctgtca cgctgctgct
    13921 gcggcggctg ccggccctgc acggcgagac gcggtccgac atcttgcggc acctgacgag
    13981 cctcaagggc gggcggctgg acaccgacga ccagtggctc gcctggtgga tggagcagca
    14041 ggcgacgttc aagtttccgg cagccggcga cgcgcccgcc cagatcgcgg cgcgtcaggg
    14101 cgaggcgagc tattacggcc tgccgctgta tggcacaaag atcgtgttca tcctcgatac
    14161 gtccgggagc atgagcgggc cgcggattct ggcggctcag cgggagctcg tttcggcgat
    14221 cgccgccctg cccgagggcg tggagttcaa cgtgctggcg ttcaactcgg cggtcgcggt
    14281 gtggcagagc cagctcgtgc ccgcctcggc ggccaccaag gaggcggcgg cccgcttcgt
    14341 catggcgcag gttgcccgcg aacagacggc gtcgttcgac gccctggatg ctgccttcgt
    14401 gttcgatgcc gaggcgatct acttcctcac cgacggtgca ccgaccaccg gcaagatcaa
    14461 gcagcccgac cagatcgtcg ccgccctcag cggccccaac cgtgtgcggc ggatcaccgt
    14521 caattgcctg ggaatcggcg tcggccaggc gggcggcccg ttcgacaagt tcctgaactc
    14581 gctcgccacg caaaacttcg gcctctatcg gcgcgtcgat caatgacgca ggtgaagcga
    14641 gcaatgcagc cgagaccctt atgcgtgtga cgtcagtgag cgtgactgtg gtggaagtgc
    14701 cgcagacggc gccgctagcg ccgtaccggt cgcacctgcg gagcagctcg acgacgcaga
    14761 gcgcgattgc ccgcgtcgaa accgatgcgg gtctcgtcgg ctggggcgag cacaacatca
    14821 acttcctgcc caacttgagc ggccggcaga tgacccgcgc ggcccagtgg ctcgtcggcc
    14881 gcgacgcgcg gaacatcagc cgcttccacc aggaatgccc gctcgagacg cggctgaagt
    14941 cggggctgga gctggccctg tgggacatct gcggccaggc cgcggggctg cccgtggcgg
    15001 cgctgttggg cggcgtgatt cgcgagcggg tcgaggtcgc cgcctgcatg ggcatccaaa
    15061 gctacgagcg ggccaaggaa atggccgcgt ggtacgtcga gcaaggcttt ggcacgctca
    15121 agaccaaggc cggctcggac atgcgcgagg acctggagat ggtccgcggc gtccgcgacg
    15181 cggtgggcga ccggctgaag ctgcggatcg atccgaaccg ggcctactcg ccgcagcagg
    15241 ccgcggagtt gggccgccag ctggagcagt acagcctcga gtatttcgag cagccgatcc
    15301 ccgcggagcc gctggccgat gcggcctggc tgcggcggca gacgcggacg ccgatcgccc
    15361 tgaacgagag cgtgaccgat ccggccagcg tgctggcaat tctgcgggcc gatgccgcgg
    15421 cgttcctcct gccggacacg cacattgccg gcggcatctg gccctgcgtt gcgattggcc
    15481 agctctgcgc ggcggccgga attccctgca tcatgcactg cggccacgac ctggggccga
    15541 agacggccgc catgctgcac gtcgccgcgg cctgtccggc atatacgctg gccaacgaca
    15601 cgacgtatta cggcctggaa gacgacatca tcaccgagcg gctctcgatc gagcgcggga
    15661 cgatggccgt gcccgcgcgg cccggcctgg gcgtcgccgg cgaccccgag cgaatcgccc
    15721 gctacgcgat cgattgctga cggctcacca gcgcctcgca tcatgcaacc cgccgacaag
    15781 ccgacgaacg tccgctgggt cgtcttctcg ctggcttgcg gcacgtcgtg gctgctctat
    15841 ctgcaccgct acagcttcgc gttattcaaa cccgagctgg tcaagcagtg ggagctcagc
    15901 aagaccgaac tgggcctgct cgacggcgcg ttctcgatcg gctcgatcgg ctttcagatt
    15961 cccttcggcg tcgcggccga tgccgtgggc gtgcgactgg tgctgacctg cctgctggtc
    16021 ctgggcagcg tcgggctggg aatgcacgcc tgggcgccct cgccaaaata cctctggccc
    16081 gcgcggctgg tcttcggcat cggccaatcg gccgtctatg cctgcctcag ccgcatcgcc
    16141 cagacctggt ttccgccccg gattcgcacg gcgctgcaat cgacggcggc gatcacgttc
    16201 ggccggctgg gcgggttgac ggcgaatctg atcttgggcg gagtggtgct ggggctgtac
    16261 ggagtcgatt ggcgtgtggc cacgaatgtg atggccgccg gcggcgtaat cttcgcgatc
    16321 gcggtggcgc tggtcttccg caattcaccg cgggaacagc cgctggccaa cgaggccgag
    16381 gccgacctga tcgatccacc gcaggcggcg accgccgaca cgccgcccag aatgtccgtg
    16441 agccaaatgc tgcggtcgct gcggccggcc gcggtgccga atttcgtctg gctcaacgtg
    16501 cagacgatcc tgagcacgtt tgccgacaat atctactcgc attggattcc gctctttctc
    16561 tgggaggtgc atcaactgaa gttcgggccg ctgggcctct attcttcgct gccgctcctg
    16621 ggcggcgcga tcgccggaat cctcggcgga atgctcaacg acggctgcat cgccgcgagc
    16681 gggaaccgcc gctggtcgcg ccgcgtcgtc gcgatctgcg gcaagggcct ggccgccgtt
    16741 ctgctcctgg ccgcgctggc ctggtaccac aatccctacg tcttctgctg gttcctgttc
    16801 gcggtgaaat tcttcggcga ctggagcctg gcgaccagct ggggcgtcgt caccgacatc
    16861 ggcggccggg ccacggcttc ggtcttcgcc gtgaacaaca ccgtcgccgg cattgggctc
    16921 ttcgccgctc cgattgtctt cggtttcgtc gccgaccact ggggctggcc ggctgtgttc
    16981 gtcgccgtcg ccgtggccta tattctgtgc gcgctgtcgt ggctcggcat cgacagcacg
    17041 cagccgctgc tggacgaccg gaagccgctt agccccggcc cccaggccgg tcagatcggt
    17101 caggtcgggt aaatctgtcc aagcgcaccg gcgtgggcgc cggggctaag cttcccgaac
    17161 gagggacatc atgcgagcaa ttctcttcac cgtatctcta gcggcgggct gcgctgtatg
    17221 tctcctagcc atcgcgaggc tgctccccgc ggacgacgca cccacgcacg tgaaggtcta
    17281 cgccgagccg ggccgcttcg gcggctggcc ggcgaatcac ggcgtctggt cgtggggcga
    17341 tgaaatcctc gtcggcttca gcgcgggcta tgccaaggac aacggccccg gtcgccatgc
    17401 catcgatcac gaccggccgg aggagcatct gctcgctcgc agccgcgatg gcggcgcgac
    17461 ctggagcatc gagaacccgg ccaaaaccgg cgcgctcatt cccaccgggg cggcgctgca
    17521 cggcgtcact ccaccgggat tgaaagagcg gccgtgggaa gattgcccgg gcggcatcga
    17581 ctttacccat cccgatttcg ccctgacggt gcgcatgacc gacgtaaacg ccggctcgtc
    17641 gcggttctat tactcgaccg accgcggcca tgcctggctc gggccgtttc gactgccgct
    17701 gttcgagcag ccgggaatcg ccgggcggac ggactatgtc gtgctcggca agcacgactg
    17761 cctgctgatg ctgaccgcgg ccaagcgcaa cggccgcgag gggcggccgc tggccgtacg
    17821 cacgaccgac ggcggacgct cgtggcagcg cctcggctgg ctcggcgacg agccggcggg
    17881 ctatgcgatc atgccttcga ctgtgctgct aggaagtgcg cggctaggag atgcgcggct
    17941 gggcgaaaac gagctgctct cggcggtccg ctgccgcgaa ggagcgcgga gctggatcga
    18001 cctctaccgc tcgcgcgacc tgggccggag ctggctgctg gccggcgcgg cagtcgctga
    18061 gacgggcgag ggtaatccgc cgagcctgat ccggctgcag ggcggccggc tctgcctcac
    18121 ctacggctac cgtgccgaac ccttcagcat ccgcgcccgc atcagctccg acggaaagac
    18181 ctggagccct gagcggacga tccgcggcgc aggcggcggc cgcgacatcg gctatccgcg
    18241 cagcgtccag cgcccggacg gcaaagtcgt gacgatctac tacttccacg accagcccgc
    18301 cagcgaccgc tacatcgccg cgacgatttg gacgccataa cgcggcgtta aacgcggccg
    18361 aagttgcgat tgaccctgcc agcggccggc cgctatagtc cgcggccttc taccccccgt
    18421 tacgacgagc cgcctacagg aatccgtggt gcgccgagtc cctacgcaac ttgttcccaa
    18481 gctccgcttg ggaacccgcg caatcgaagc tctgcttcga ggccgacgaa gcggagcttc
    18541 gcggacgggc gttcccaagc agagcttggg aacgaggtgg cgaatccttg ctgtgttgct
    18601 ggttgtcgta ccctgcgcct catcgttgcg cgccgagaac cgctggcccg acgagcggca
    18661 agccgggccg ttcctctgcc atgccgattt cgccctcgcc ccgcagcagc cgctgctcga
    18721 cgagctgtcg gcgctgcggc aggacttgca ggcgattctc ggcacgccgg agtcggccga
    18781 cccggtgcat ttgttcctgt tccaggagaa gccgacttac caggcatacc tcaagcagca
    18841 tttcccccgc gtgccctacc ggcgggcgct gttcatcaag ggccgcggcc cgggaatggt
    18901 gttcgcgttc caggggaccg acttccagat cgacgtccgc cacgagtgca cgcacgcgct
    18961 cttgaacgcc tggctgccgc gggtgccgct gtggctcgac gagggcctgg ccgagtattt
    19021 tgaagtcgag cgcgacaagc gctccgccgg caatccccat caccaggtcg tccgggccat
    19081 gttccaaagc ggccagcgcc cgcggctgga agagctggag gcgatcgaga ctctggacgc
    19141 gatgggccgc gatgaatatc gcgacgcctg ggcctgggtg cacttcatgc tccacggccc
    19201 gcggccggcg cacgacgagc tggtccgcta tctgggcgag ctcgaatccg gcggcgaaag
    19261 cggccggttg agcgaccgcc tcaaacgacg gctgccggat ctcgaccgcc ggctcgatga
    19321 gcactttcgc tagtagtcct cacgctccgc gtgaggccca catctcgcgg agcgagatga
    19381 ctacctatag cgcccccggt tcggcgtcag atcgtgacga ttcgagatac gccgccagca
    19441 gaatctgcgc ggcgagcttg tcgagccgct ccctgcgccg cttcttcgtc aggccggctt
    19501 cgagcaaggc ggcctcggcg tgggccgtcg tgtaacgctc gtcgaacagc cgcacgggca
    19561 acttcgtctc tttcgccagc caggcagcga attgccctgc ctcgcgggac ttctggctct
    19621 cttcgccgct gccgtgcacc ggcaggccga cgacgaaacc aacgactcgc tcgctcgcgg
    19681 ccagccgctt gatccaggcg gcgtcggcga ccaggccatt gcgggtgtaa ttctcgtgcg
    19741 ggctggccag gcgctggtcg gggtccgtaa tcgccacgcc gatccgcacc gtgccgaagt
    19801 cgatgcccgc caggcggccg gtccgcggca ggtcatcagg ccgcgcgtcg ctcatggcgc
    19861 tgccttggcg gccaccacga tcggctgccg ctcgccctcg gcggtgaaat agccgtgctg
    19921 gaggaacgcc aggacgcacg cgcgggcggc gggatcgtcc atgagcaggg cgtggcggca
    19981 gtgcaacagc cggaagtcgg ccgctccggc cagccgcgtt tcctcgaccg agacgatcag
    20041 gtcgtcgtcg ccggggacga gcgggttgag gccgcggtcg ttgccgctgc cgccggccag
    20101 gatgccgaac ggaaagctcg gcgtggcgag cgtcttttgg cattcgtccc agctgcgggc
    20161 gagttgcttg ccgctgggcc cggtgaccag gccgaacagt tcgttgttgc ggaatagcac
    20221 ggcgagccga gccccattgt tgggcggccc gagcatcacc atccgcttga tccgcggatc
    20281 gacctgccaa cggggctcgc tctggctggc ctcgcccagg tagcggcgga cgacgaggtt
    20341 gcccaggctg tggccgacga agtcgatttc ctcaacgccc tccagcccgg cgagaacgcg
    20401 cgacagggag agtgcgtggt cgtcgagcga gcgccgcgtg ctggcatagg agacgttgac
    20461 gacgtggtac ttgccttcct cggccaggaa tttgcccagg ccgtccatgt ggtcgcgcga
    20521 gcggatgagc ccgtgcagcg tgacgacggc ccgaccgctc aaggggggca ggttctcgtg
    20581 ccgcttgaac tcggcgaatg ctgcccggca ctgctcggcc gttccccagg cccgccgcac
    20641 gtcgcgcggg tcgagcaggc gatagtgttg cgtcagcacg ttctgctgaa tccgccagcc
    20701 ctgataaacc agctcgtcgc tccagaactg ctcgccgccg agcgtcggca gcggcacgtt
    20761 gaccggcggc ggatcctcgc cgcgcagcgg cgccgcgaac gctggtgcaa aggccaggaa
    20821 tacgaacaag tgcgcacgca tggcactcat tatatcgaac gagcgacgcg tggcactggc
    20881 gtctcgccag tgctctgcgg gtcttctgga taacggttac actgcggcct gcaagtttgc
    20941 cctgctccaa aatggcccat tggtgcggga gccccagcgc tcgcgcttgc tccttcgctc
    21001 ggcactggcg acacaccagt gtcacgcggc cagaatggac cacactagtc cattccgcta
    21061 gcgggggcga aaaaaagttg cgtcacgggc cagcgacgga agtaacggcg ggcattgact
    21121 ttgcagccag gccaatcgac gcaagttgcc atttactgaa caatatgtgt acaagttgct
    21181 tgccaaccgc gcctcggcgc ggataatggc acactcggag ggcgaagctc ctgctgagct
    21241 tgatggtcgc ggctccacgc gcacctcgcc ctcccttcga gattgtccgt tctttctcaa
    21301 ttcggaccga gtcaacattc agcctaagcg gcggcggacg attttttcgc catggcgaaa
    21361 aaatcaaagc cagacgcaga ccgcacttgc gccaatggga gcgaattatt cgccagatcc
    21421 ccaggtatcg ggcctcaatg gcgaattatt cggggccatt gcgaggaagg cgaaagtcac
    21481 aagtcgagtc cgcggcgtac tttgcatcaa cgcacctggt cggcatccgc ccgattaatt
    21541 caccacggag cttgccgaag cggtgaataa cggcagggcc aaagccagcg ccgctgcgcg
    21601 gccaacttca gctggcgtta cgccgcgcgg cggagcgcgc gactggcggc gccggcacgt
    21661 tgctgcacgt ccgcgatcgt cagcttgcgc caaccggttg cacccgcctt ggccggctcc
    21721 tccaggaaca cggacgattg ggcggtgagc atccgcggca gccaggtcca ggcccgcgcc
    21781 agcgactcgg cgtccaggcc ggcggaaatg agcagcagct ccgtgcccgc tagcgcgtta
    21841 gcgacgcgcc gcagcgcgac gtcggcttcg ccaggcacta gctgcacctt tacgcccggc
    21901 cgctggaaca gcgcgaacgc ctccttgagg gagtgtccgg gactgccggc cgggcgggcc
    21961 tcgaacaggt cgatccccgt atagcgcaga ggcaaagagg ccgcccgcca ggcggcgact
    22021 tccagcagcc gctgcgtgcg gccgccggag ccgatgccga tttcgacgat cgagcggatc
    22081 ggccggcttt tgacggcccg gtagaccgcg cggtcgctag cgggctggga aaaatgcagc
    22141 aggtacagac tgcggagcag cgtgccaacg ctcaaggtcg cattcctttg cggccggaga
    22201 ggttcactcc ctatctttcg tcggccaggc ctgatgcctt cattccgcat tccgcactcc
    22261 gcattccgca tttgcccgct accggttctc caggtcgcgc cggtcgcgca ggccgcaaac
    22321 tcccgcgcct gccaccacgg aaaccgccgc tggtccgctc gcgtaggaca tgacctagat
    22381 ttgctggctt cagccgcacg gacgattccc ggctgctcaa cccctcgtgc ccggttccgg
    22441 gcgaagcggc tgcgagcgac aggctccccc tgttggttcc agtttcgcgc tgaaggtgcg
    22501 cgccgctgtc cggaccggtt tcctcgcacg tacacgaggc aggtccgatg cagcgacagg
    22561 gcgaagccga gcgcgagcgc ggctggaagt tcgagacgtt cgaagagcgg ctggccctct
    22621 ccgccgagcc gctggcggac ttctggtacg acctcgatcc agagacactc agcgagcctt
    22681 cgtcggccgt tgttcagccg ctggccgcgg aagggcacgg ctggaccgac gtcgccggcg
    22741 tccgcgagca gttcggcctg cggggcggcg accagacggt ggcgatcatc gacagcggca
    22801 tcgcctacga ccacatcgcg ctgggcggcg ggctgggcaa ggcctaccgc gtggtcggcg
    22861 gctgggactt tgccgagaac gacgccaacc cctacgacga cggcccggcg ggctttcacg
    22921 gcacgcacgt ggcgggaatc gtcggggcga tggacggcaa gtatccgggc gtcgcgccgg
    22981 gcgtcgatct tgtcgcactc cgtgtgttca acgaccaggg gggcgggaac tttgcctggg
    23041 tcgagcaagc gctgcagtgg gtccatacga accgcacggc gtttgacaac ccgatcacga
    23101 cggtcaatat ctcgctgggc accgagtgga attccgccgc gctcccccaa tgggcgacgc
    23161 tggaagaaga gctggcgcag ctggcccggg acggcatctt catctcggtc gcggcgggga
    23221 attcgttcct ggcttacggc actcccggtt tgtcgtatcc cgccgtcagc caaaatgtca
    23281 cgcccgtggc cagcgtcgat ggcagcggca acctcagccg ctacacgcag cggagcgagc
    23341 gggtgctggc cgcgccgggc gagcgaatca tgagcacgct gcccgatcac ttctacggcg
    23401 gcgacggcgt gaagaacgat tggggagcga ccagcggcag cagcatggcc gcgccctacg
    23461 tcgccggggc gagcgtgctc gtccgcgagg cgatgcagga tctgggcgtc acgaacatca
    23521 cgcaagggac gatctacgac ctgctgcgac gcacggccga cacgatctac gacgcggcga
    23581 ccagcgccag ctacaagcgc gtgaacctga cgcgggcgct tgagacgctg gtgggtgttg
    23641 acgattacgg ctcaaccgcc gaagccgcca catcgctggg ccgtctggcg accagccagc
    23701 gcgtcagcgg cacgatcggc cgagtgggcg acgaggactt tttccaaatc accgccggct
    23761 cgaccggcca gttg
//
LOCUS       DASRJB010000146         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3425254, whole genome shotgun sequence.
ACCESSION   DASRJB010000146 DASRJB010000000
VERSION     DASRJB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425254"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1911)
                     /locus_tag="VFB80_04185"
     CDS             complement(217..1911)
                     /locus_tag="VFB80_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN32988.1"
                     /translation="MPESMFDAYRPLSRRTVLASLAGLGLGTAAFQRALAAQAEQAGQ
                     VTPEMITQAEWIAGITLTEEDRKSVAGAVQRDRRRYETLRAVALANSVPPALAFFAAP
                     PQQAGAEVRRGDVRPIESAAPERPPADEDLAFLPVTELAALVRTKKVSSVELTKLYLS
                     RLKRYDDLLKCVVSLTEEVAMEQAERADREIAAGRYRGPLHGIPWGAKDIISWPGYKT
                     TWGAGHYQEQKLDTKATVARRLEEAGAVLVAKMTVGSLALGDQWFGGMTRNPWNPEEG
                     SSGSSAGSTSAVVAGLVGFALGTETLGSIVSPCRRCSASGLRPTFGRVSRAGLMTLSW
                     SMDKVGPIARSIEDCALVFGAIHGSDPDDITAVDRPFAWPPAQDVRSLRVGFFEGERP
                     PEEKRVLDRLKDLGVKLVPIKLPDKYPVGPLTLILNTEAAAAFDDITRAGVRDGIGRW
                     GTTFRQGQFVPAVEYLRACRIRTLVMREMEEAVAGVDAYVGGDDLTITNMTGHPTVVV
                     PDGDRRNSMTGQPDTITFTGRLFGESALLGLAHAYQQASGAHLRRPPLEKLLAAKS"
BASE COUNT          361 a          785 c          761 g          367 t
ORIGIN      
        1 cagccacctg ctggccacga tcatgcacac gctgttcgac ctgggcaagc tgcggctcga
       61 ccagagcctg ccccgcgacg tcgccagcgt gatcctcggc ggcgagccca tcgggccgct
      121 gctgtagggt gggtcgaggc cgctgcggcc gaagacccac gcggctgaat actcgcctcc
      181 gcgtgggtct gcgctcgcgg actcgcttga cccaccctac gattttgccg ccagcaactt
      241 ctccagcggt ggccggcgga ggtgagcgcc gctggcctgt tgataggcgt gggccaggcc
      301 cagcagcgcc gattcgccga acagccggcc ggtgaacgtg atcgtgtcgg gctggccggt
      361 catgctgttg cggcggtcgc cgtcgggcac gacgacggtc ggatggccgg tcatgttggt
      421 gatggtcagg tcgtcgccac caacataggc atcgacgccg gcgaccgcct cttccatctc
      481 gcgcatgact agcgtgcgga tccggcaagc gcggagatac tcgactgcgg gcacgaactg
      541 cccctggcgg aaggtcgtgc cccagcggcc gatgccgtcg cggacgcccg cccgcgtgat
      601 gtcgtcgaac gcggcggcgg cttcggtgtt gaggatcagc gtcagcggcc ccaccggata
      661 tttgtcgggc agcttgatcg gcaccagctt gacgcccaag tccttcagcc gatcgagcac
      721 ccgtttctct tcaggcgggc gctcgccttc gaagaagccg accctgagcg aacgaacgtc
      781 ctgcgccggc ggccaggcga acggccgatc gaccgctgtg atgtcgtccg gatcgctgcc
      841 gtgaatcgcc ccaaacacga gcgcacagtc ctcgatcgag cgggcaatcg gcccgacctt
      901 atccatgctc caggagagcg tcatcagccc cgcccggctg acgcggccaa acgtgggccg
      961 caggccgctg gccgagcagc ggcggcaagg cgagacgatg ctgcccagcg tttcggtgcc
     1021 gagtgcaaag ccgacgagcc ccgcgacgac ggcggaggtc gagcccgcgg atgagccgct
     1081 ggagccttcc tccggattcc agggattgcg ggtcatgccg ccgaaccact ggtcgccaag
     1141 ggccagcgat ccgacggtca tcttggcgac gagcaccgcc ccggcttctt ccaggcggcg
     1201 ggcgacggtc gccttggtgt cgagcttctg ctcctgatag tgccccgcgc cccaagtcgt
     1261 cttgtagccc ggccaagaga tgatgtcctt cgctccccag ggaatgccgt gcagcggccc
     1321 gcgatagcgg ccggcggcaa tctcgcgatc ggcccgctcg gcctgttcca ttgccacctc
     1381 ctccgtgagc gagacgacac acttgagcag gtcgtcgtag cgtttcagcc gcgagaggta
     1441 gagcttcgtc agttcgaccg aagagacctt tttcgtccgc acgagggccg cgagctccgt
     1501 caccggcaga aaagccaggt cttcatccgc cggcggccgt tccggcgctg ccgactcgat
     1561 tgggcgcacg tcgccgcggc ggacttccgc gccggcctgc tgcggcggcg cggcgaagaa
     1621 ggccagcgcc ggcggcacgc tgttggccag cgcgaccgcg cggagcgttt cgtacctccg
     1681 ccggtcgcgc tgcaccgcgc cggcgaccga cttgcggtcc tcctcggtga gcgtgatccc
     1741 ggcgatccac tcggcctgcg tgatcatctc cggcgtgacc tggcccgcct gctcggcttg
     1801 cgcggccagg gcccgctgaa acgcggcggt tcccaggccc agccccgcca gcgaagccag
     1861 gaccgtgcga cgcgaaagcg gtcgataagc gtcaaacatg gattcaggca tggtgcgatt
     1921 ctcgcggctg gtggatgcga atgccacgat tgttcgtcgc cgctgggccc atcgcaaggg
     1981 tccgattagc gggaagccca gaatcgctca agagtctggc cgcaacagaa tgacgatcgt
     2041 gtcgccaccg cgggcttcgc agcggaggcc gagctcatcc aagagcaggg ccagcacgtg
     2101 cttgaaaggg agattcttca agcttttgct cacccgcggg gcccgaatcc cgcgcgtctc
     2161 gagtccaact ccaaatcgcc ggcgtggaca ccggggctaa gcggtgacca ggccggttgg
     2221 ccgaattttg gctccgccag cggctgccag aatggaccac actagtccat tccg
//
LOCUS       DASRJB010000147         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1734103, whole genome shotgun sequence.
ACCESSION   DASRJB010000147 DASRJB010000000
VERSION     DASRJB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734103"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFB80_04190"
     CDS             <1..862
                     /locus_tag="VFB80_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN32989.1"
                     /translation="LILRDTMRRTGRVFQVGTQRRNLPHFAFACELARTGKLGKLQRI
                     YAHPRGMLALTSGWFPAEPEPDKRVTDWDMWLGPAAWRPYNSRLVDGFNFEKGGGMIG
                     GFEFDAMGRKTIGGGVLEWGSHCVDLCQWAVDDCPPPVEYNPPKDGQIVCKYQNGVEL
                     IFREEGWIPLGSCPVRFEGETGWVEAGDSGQIRLSSPALLAGRTVEEIGGYPATFHVR
                     DFLDCVKTRGVPKGNAEAACNAHIVCHAANIALYLQRQVKYDIAKNEFIGDEPANRLR
                     SEALREPWRL"
     gene            973..2838
                     /locus_tag="VFB80_04195"
     CDS             973..2838
                     /locus_tag="VFB80_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN32990.1"
                     /translation="MNTRRISIVLALLVLAIARPGAAQQISAEKERALIAILKSDAPA
                     AEKAIACKRLSVDGSSEAVPELARLLGNEQLASWARTALEAIPGAASDEALRKATDSL
                     QGRLLVGTINSIGVRRDAGAAQLLAGRLEDNDADVAAAAAVALGRVGNAAAAESLRKA
                     LAAAPPAVRSAVAEGLVLCAESALAKGDAAGATKLYDEVRSAANVPRQRMLEATRGAI
                     LARGQEGIPLLVEQLRSSDKGLFQIALGTAREFPGRDIDKALAAELDRAPPERAVLVV
                     LAMADRKQTVDLPAVLKAASSSAKPVRLAAIGALARVGDATCLVPLLESAAGDDAEIA
                     AAAKSALAALPGQAVDREIASRLARAEGKTHPVLIELVGQRRIEATEALLSALKHSDK
                     AVRTSALTALGNTVTDKHLPVLIAQVVTPTDSESAPVAQAALKTAAVRMPDREACATE
                     LAAALSRAPAGTKIVLLDILAAVGGTKALAAVGAAGKSPDNALQDASTRLLGEWTTID
                     AAPVLLDLAKTNGSYQTRALRGYIRIARQFTMTDPQRVDMCKSALDAARQPAEKKLVL
                     EVLKRYPSVEMLKLAASAAQAAELKADAADAAKAIAEKLPKTDEVRQLLSKAGVP"
     gene            2947..4110
                     /locus_tag="VFB80_04200"
     CDS             2947..4110
                     /locus_tag="VFB80_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN32991.1"
                     /translation="MIATLAMFAVAGLMADDVTVKLVPSGVTEKVGGYSPLRAEMNGK
                     AELVKKAPEGLTAPKYGTIKIGTKGWTFILDEPEGQPAKLYVDANGDGDLTNDPATTW
                     DSSTTNGLTQYRGKAQVDLGEGKLVGLGLYRFDPKDTGRAQVKNTLMYYTDFGYEVTL
                     DLDGKPHTSFFSGDAAKVRMLWVDRDGNGKQSYKLETIQVGKPFNYTGTTYVLNAGAG
                     GLKLEKSSESLPKAPLPPVLTIGKQALPFEMASTSGNKIVFPKAYAGKLVMLDFWATW
                     CGPCIAELPNVKKAYADWHDKGFEIVGISFDRADMGEKLKKFTTENDMPWEQIYEGKY
                     WDTSLGELYDVGGIPFVLLVDGDTGEILANAEQLRGPGCSEYIGKALAKKNGK"
BASE COUNT          769 a         1338 c         1400 g          625 t
ORIGIN      
        1 cctgatcctc cgcgacacga tgcgccgcac gggccgcgtg ttccaggtcg gcacgcagcg
       61 gcggaacctc ccgcactttg cgttcgcctg cgagctggcc cgcaccggca agctcggcaa
      121 gctccagcgg atctatgctc atccgcgcgg catgctggcg ctcaccagcg gctggttccc
      181 cgccgagccc gagccggaca agagagttac tgattgggac atgtggctcg gcccggccgc
      241 ctggcggccg tacaactcca ggcttgtgga cgggttcaat ttcgagaagg gcgggggcat
      301 gatcggcggc ttcgagttcg atgcgatggg ccgcaagacg atcggcggcg gcgtgctcga
      361 atggggttcg cactgcgtgg acctgtgcca gtgggccgtc gatgactgcc cgccgcccgt
      421 cgaatacaac ccgcccaagg acggccaaat cgtctgcaaa taccaaaacg gggtggagct
      481 gatcttccgc gaggagggtt ggattccgct cggctcctgc ccggtacggt tcgagggcga
      541 gacgggctgg gtggaagcgg gcgacagcgg ccagattcgc ctcagctcgc cggcgctgtt
      601 ggccggccgg accgtcgaag agatcggcgg ctacccggcg acgtttcacg tccgcgattt
      661 cctggactgc gtgaagaccc gcggcgtgcc gaagggcaat gccgaggcgg cctgtaacgc
      721 gcacatcgtc tgccacgccg ccaacatcgc gctctacctc cagcggcaag tgaaatacga
      781 catcgcgaag aacgaattca tcggcgacga gccggccaat cggctgcgca gcgaggcgct
      841 gcgcgagccg tggcggctgt aaatggttgg tcagtagtca gtgggccgtt gtcagttgga
      901 aggatcccaa tcgactttgc caactgacca ctgaccactg acattcagca aacaactcat
      961 gtgggttcaa ccatgaatac tcggcgaatt agcatcgtcc tggctctcct ggtccttgcc
     1021 atcgcccggc ccggggcagc acaacagatt tcggcggaga aggaacgggc gctgatcgcc
     1081 atcctgaagt ccgacgcgcc ggcggcggag aaggccattg cttgcaagcg tctatccgtg
     1141 gacggctcca gcgaggcggt tccagaactg gccaggctgc tgggaaatga acagctcgcc
     1201 tcgtgggcgc ggaccgctct ggaggcgatt cccggcgcgg cgtccgacga agcgctccgc
     1261 aaggcgaccg actcgctcca gggcaggctg ctggtcggta cgatcaactc gatcggcgtc
     1321 cgccgcgacg cgggcgccgc gcagctgctg gctggtcgtc tagaagataa cgacgccgac
     1381 gtcgcggctg ccgccgcggt ggccttgggc cgcgtcggca atgccgcggc ggccgaatcg
     1441 ctccgcaaag cgctggccgc cgcgccgccg gcggtccgct cggcagtcgc ggaagggctc
     1501 gtgctgtgcg ccgaaagcgc cttggccaag ggagacgccg ccggcgctac gaagctctac
     1561 gacgaagtcc gcagcgcggc gaacgtgccg cggcagcgca tgctggaggc gacgcgcggg
     1621 gcgatcctgg cccgcggcca ggaaggcatc ccgctgctcg tcgagcagct tcgttcttcc
     1681 gataagggac tgttccagat tgccctcggt acggcccgcg agttccccgg ccgcgacatc
     1741 gacaaggccc tcgcggcgga gttggaccgc gccccgcccg agcgggcggt gctggtcgtc
     1801 ttggcgatgg ccgaccgcaa gcagacggtc gatctgccgg ccgtgctgaa agccgcgtcg
     1861 agcagcgcca agcctgtccg cctggcggcg atcggcgcgc tggcccgtgt cggcgatgcg
     1921 acctgcctcg tgccgctgct ggaatcggcc gccggagatg acgccgaaat cgccgctgcc
     1981 gccaagagcg ctctggctgc gctgccgggc caggccgtgg accgggaaat cgccagccgc
     2041 ctggctcgcg cggaaggcaa gacccatccg gtgctgattg agctggtcgg gcagcggcgg
     2101 atcgaagcga ccgaggcgct gttgagcgcg ctcaagcact ccgacaaggc ggtccgcacc
     2161 tccgctctca ccgcgctggg taacacggtt accgataagc atctgccggt gctgattgcc
     2221 caggtggtga ctcccaccga ttcggaaagc gcccccgtcg ctcaagcggc cttgaagacc
     2281 gccgccgtcc ggatgccgga ccgcgaggct tgtgcgacag agctggcagc cgcactgtcc
     2341 cgcgcacccg ccgggaccaa gatcgtcctg ctggatatcc tggcggcggt cggcggaacc
     2401 aaggcgctgg cggcggtggg cgccgcggga aagagccccg acaacgcgct ccaggacgcc
     2461 agcacgcggt tgctcggcga atggaccacg atcgacgccg ctcccgtgct gctcgacctg
     2521 gccaagacca acggcagcta tcagacccgc gcccttcgcg gctacatccg catcgcgcgg
     2581 cagttcacga tgaccgatcc gcagcgggtg gacatgtgca agagcgccct ggatgccgcg
     2641 cggcaacccg ccgagaaaaa gctcgtgctg gaagtgctga agcgctaccc cagcgtcgaa
     2701 atgctgaagc tggcagccag cgcggcgcag gcggccgagc taaaggccga tgcggccgac
     2761 gctgcgaagg ccatcgccga aaaactcccc aagacagacg aagtgcgcca gctactctcc
     2821 aaggctggcg tgccctagcc gtcgcaaaag tcgcggattg accctgccct gagcgggcgg
     2881 tatggtctag tagtgtcctg tcgcttgtag aaaaaacgtg cccgtctatt tccggagtcc
     2941 gaatcgatga tcgccaccct cgcaatgttc gccgtggccg gcctgatggc cgacgacgtg
     3001 actgtgaaac tggtgcccag cggcgtcacc gaaaaggtcg gcggctacag cccgctgcgg
     3061 gccgagatga atgggaaggc cgagctggtg aagaaggccc cggaagggct gaccgcgccg
     3121 aagtacggca cgatcaagat cgggaccaag ggttggacgt tcatcctgga tgagcccgag
     3181 ggccagccgg cgaagctcta tgtcgatgcc aacggcgacg gcgacctgac gaacgaccca
     3241 gcgacgacgt gggactccag cacgacgaac ggcctcacgc aatacagggg caaggcgcag
     3301 gtagacctcg gcgaaggcaa gttggtcggg ttgggcctct atcgcttcga tcccaaggat
     3361 accggtcggg cgcaggtcaa gaacacgctg atgtactaca ccgacttcgg ctacgaggtc
     3421 acgctggacc tcgacggcaa gccgcatacg tcgttcttct cgggcgacgc ggcgaaagtc
     3481 cgcatgttgt gggtcgatcg cgacggcaac ggcaagcaga gctacaagct ggagacgatc
     3541 caggtcggca agccgttcaa ctacaccggc acgacctacg tcctgaacgc tggcgccggc
     3601 gggctcaagc tcgagaaatc gtccgagtcg ctgcccaagg cgccgctgcc gccggttctg
     3661 acgatcggca agcaggcgct gccctttgag atggcctcga ccagcggcaa caagatcgtg
     3721 ttccccaagg cctacgccgg caagctggtg atgctcgact tctgggcgac gtggtgcggt
     3781 ccgtgcatcg ccgagctgcc caatgtgaag aaggcctacg ccgactggca cgacaaagga
     3841 tttgaaatcg tgggcatcag ctttgaccgt gccgacatgg gcgagaagct gaagaagttc
     3901 acgaccgaaa acgacatgcc gtgggagcag atttacgaag gcaagtactg ggacaccagc
     3961 ctcggcgagc tgtacgacgt cggcggcatt cctttcgtgc tgctcgtgga cggcgacacg
     4021 ggcgaaatcc tggccaacgc ggagcagctc cgcgggcccg gctgctcgga atacatcggc
     4081 aaggccctgg ccaagaagaa cggcaagtag cgctaccgca gctcgacggg aa
//
LOCUS       DASRJB010000148        11500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1452354, whole genome shotgun sequence.
ACCESSION   DASRJB010000148 DASRJB010000000
VERSION     DASRJB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452354"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFB80_04205"
     CDS             complement(<1..594)
                     /locus_tag="VFB80_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN32992.1"
                     /translation="MFVRFASVALCACLATASLAAEEAFSNAKDAGPDYAVQGEYKGG
                     AGDQVWGAQVIALGDGKFDVVGFRGGLPGDGWKRGDQTRRGKGETKDGTVQVQGDTWT
                     ATIKDGVFTVMQDGATIGELKKVERKSPTLGAKPPAGALVLFDGSTADSFENGKIIED
                     NLLGATGCVSKKAFGDHSLHIEFRTPFVPKGRGQGRGN"
     gene            899..1432
                     /locus_tag="VFB80_04210"
     CDS             899..1432
                     /locus_tag="VFB80_04210"
                     /inference="COORDINATES: protein motif:HMM:NF039424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32993.1"
                     /translation="MSFAGFLAALLEHGRVKVAHPQEHVPSDEIAAAGRQFQERAALV
                     AAEFPGQPPALDVAVAQWAALQMYRACQLAIYRELDAGAIDELLAPACPPAEPASRHW
                     SADLAFVFLPDLIRQAQAAAANDPLVTRLREWAADWPLSSVGVAQVEPRNVTEIAGHP
                     GLLRLYVDRILSKKDWP"
     gene            1531..2685
                     /locus_tag="VFB80_04215"
     CDS             1531..2685
                     /locus_tag="VFB80_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN32994.1"
                     /translation="MTDPRPHLARRLATDVLASLKNAFVGKDEIIDLMGVCLAGGENL
                     FLLGPPGTAKSALVQQLARRIDGRVFDYLLTRFTEPNELFGPFDIRKLREGELITNTE
                     GMLPEAAVVFLDELLNANSAILNSLLMVLNERVFRRGRETRSLQLLMVVGASNRLPED
                     DALAALFDRFLLRVRCDNVPEDRLGEVLTAGWKLDRGLTGEHATLSVEEIAAVQQLVF
                     EVDLSPIQRPYVELVHKLRQAGIPVSDRRAVKLQRLMAASAALCGRTAADTTDLWILR
                     YIWDVEEQREVIAAIVNQAVEASPPEAQATSHPRAKQSSGPDPEELARDLVRIGQRLA
                     DPALAAAERAVLRDQLGLLAGRCQWVVSRQQRDFLDQQVATLWTQFGVRP"
     gene            2682..2993
                     /locus_tag="VFB80_04220"
     CDS             2682..2993
                     /locus_tag="VFB80_04220"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32995.1"
                     /translation="MSGTWALAAPRESLPAIARLRQVPGLAVCEESQRLWLRGPELDD
                     ALAAALKLIPGGCQFAVLQDGQLIAAGKLVPSGHLPAGPWQLLADWLTLELPPLQPPG
                     V"
     gene            complement(3012..3695)
                     /locus_tag="VFB80_04225"
     CDS             complement(3012..3695)
                     /locus_tag="VFB80_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HZN32996.1"
                     /translation="MMGTADTATLRTPQDARRRVDRLLHSREEREQRSIDLAKKFTEL
                     NAYLDIAGDVTVALEQLSDQLFQQLLVTVQEKLTIALREILEQPIEFKAAADFKRGQA
                     TVEFWVERGGDKEDILRGQGGSVANILSVGLRMFALATLDPSRHRRFLVLDEQDCWLR
                     PDLVPRLVKIVHEAGRALGFQVLMISHHDVALFERYADRIYQFEPGGDGGVAVRLLNA
                     PAADEDAAG"
     gene            complement(3692..4012)
                     /locus_tag="VFB80_04230"
     CDS             complement(3692..4012)
                     /locus_tag="VFB80_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN32997.1"
                     /translation="MANKAASNGQQSIEELQERYARLHTKKIQADTNLLHARNQLEQL
                     RKEAREKYGTDDLAELRKKLGQMQSENEAARAGYQQELERIEADLAAVEQKFAETKSA
                     EKPA"
     gene            complement(4002..5003)
                     /locus_tag="VFB80_04235"
     CDS             complement(4002..5003)
                     /locus_tag="VFB80_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN32998.1"
                     /translation="MPSYKQNTLTLTLSQGEREPEGAYTGLLVIGDPHLEGRQPGFRK
                     DDYPSVILEKLAWCLDYAAAHRLLPAILGDLFDKPRDNPNWLIARLLDLLRGEVIGLF
                     GNHDCANPQLSDDDSISLLVKSGRLRLIDAEHPWIGTLGGREAVIGGSSYRQPIPKVF
                     EPPAAAGRQPLVIWLTHHDVLIPGYDEGRIQPCEIQGVELLINGHIHRRLEEVRVGAT
                     RWLTPGNITRRSRNEATRMHVPAALRIDVSPASYKLTFVEVPHRPFDDVFHEAVADLP
                     ASAAGSAFVAGLAELQARRTGSGAGLRPFLEQNLAQFPPAVAAEIMNLASEVLSDGE"
     gene            complement(5014..6372)
                     /locus_tag="VFB80_04240"
     CDS             complement(5014..6372)
                     /locus_tag="VFB80_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN32999.1"
                     /translation="MLTRIELTNFMAHAHTVIEPAAGLTVLVGPNNCGKSAVVAALQI
                     LCHNDPSTYVLRHGEKECSVQVTTDDGHTALWKRKKTGASYEIDGEKFDRLRGADVSE
                     PLHRALRLPLVEGSGEADFDVHFGTQKSPIFLLNSSSATAARFFASSSDASRLVEMQS
                     RHKARLAERQREKNRLEAESKQLTAELETLQPAVDLDKRLKAAEQQHAELLRRSGELE
                     QAQRAAAALDAKQAEAAQVAALAASLEPLAPPPKLPPTQPLADLIDALQRAAAAHEQA
                     AARAQQLAPLAAPPAMSDVAALARTADELAALDRAAARAEAERQRLSAICEPPRLADS
                     AALAKLIENLFAAAAQAMRWESAASVAGIEAPRPQLELAPLRDFAGKFEAALALVAES
                     AREEAAALAELNAAATELRAAADGEVCPTCGVPLDPERLLAHAATGNRNATEGVPYRG
                     PR"
     gene            6573..8102
                     /locus_tag="VFB80_04245"
     CDS             6573..8102
                     /locus_tag="VFB80_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN33000.1"
                     /translation="MSSPVSTAKPLTFTQWMICTMAAIGFAFDIYELLMLPLIIRPSL
                     LELTGATPGTPTFNYWGGMLFYVPAVAGGIFGLMGGYLTDRLGRRRVLTWSILLFAFS
                     KFFAGFSTSVWVLLILRSTAFVGVCVEFVAAVAWVAELFPDPKQREKALGYTQAFSSL
                     GGLMIAIANGLCIQFANQLPPIFIPEALTGIFGTIEVAHQHEPWRYTLISGLIPAIPL
                     IIIRPFLPESPVWAEKRKAGTLKRPTILALFSPELARTSIVTAIGFACTLGIAFGAIQ
                     QMPQIVPGLPEVQAGAKKASDAAVAKAKAKGEANADTLAAVARGVGGAYRQKIASEYT
                     KMQEVGGLIGRFLIALVLAAGVLRWGNALRLFQLPGLILVPLVFWFFLRAPNRTFFEI
                     PLEAVYLGTVPVTTMSVGMFLVGMFVVAQLSFWGNYLPHVYPIHLRGTGESFAANIGG
                     RLIGTFAAMLTWSLSGLLPYKDGPLQFSTAAAIVGTTIAVIGFLASFWLPEPHPKGGD
                     E"
     gene            complement(8252..8404)
                     /locus_tag="VFB80_04250"
     CDS             complement(8252..8404)
                     /locus_tag="VFB80_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33001.1"
                     /translation="MLADLLMLAMALGIVLVPVIWMTRPLRLVNPYSQCGPRLPRYPL
                     LTEATL"
     gene            complement(8660..9052)
                     /locus_tag="VFB80_04255"
     CDS             complement(8660..9052)
                     /locus_tag="VFB80_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33002.1"
                     /translation="MKSLTILSLLAGLLIAAAATPALAVHPHHGYHAIRADVMARYYA
                     SQRPWHGQWAYTPWGMPTALVVPPNSKMHTSYAWGVGQSQMIPIYHQFFRDYPGELAV
                     GGGAEIYATPYWPSHTDQFGVYPVRGPW"
     gene            complement(9102..9683)
                     /locus_tag="VFB80_04260"
     CDS             complement(9102..9683)
                     /locus_tag="VFB80_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33003.1"
                     /translation="MLRSILGSAALLALLASAAHAQKIETPANSEYYAGADVQSDGSC
                     PDGYGANGGHGGVFGRHGRVDAAPLSIMSGYPDGVTNYSDRAYARAWAGPGGCRTCNN
                     GNCQYRFYGAPDLFYNYYAWPSCTGLGAELYLSPRPVPPHVGHTYITYQPLYPHEFLY
                     THHRTYHQYYNGGQGLNRTKVHWTWSPFGQIGY"
     gene            10118..>11500
                     /locus_tag="VFB80_04265"
     CDS             10118..>11500
                     /locus_tag="VFB80_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN33004.1"
                     /translation="MDAAANATRSNGAANGQPHADAQSPAAHEEVDHGRGKRRATAES
                     MAAKQRDISVSEFFAKNRHLLGFDNPRKALLTTVKEAVDNSLDACEEAGIVPEIWVTI
                     EATGNNRFKVSVQDNGPGIVKKQIPLIFGKLLYGSKFHRLRMSRGQQGIGISAAGMYG
                     MLTTGKPVKIISKISPKKPAHYFELQIDTKRNHPEIINGKGEGVDFSPGEKGHQEMLK
                     QGVDWVSHYPGAQGKEPQEVKSGTRVTIELEGRFIRGRGSVDEYLEQTAIANPHVTLH
                     YKDAEGNESSYRRSTTQLPPEPKEIKPHPYGIELGRLVTMLKDSTAGTMSAFLTESFS
                     RVSSGVARKICEEAKITTRSNPRRIGRQEADSLYQAIQTTRISSPATDCLVPIGEQLI
                     LKGLHQVVPGEFYCAATRPPAVYRGNPFLIEVGLAYGGVSTAQKVPLDTLTEMLGETD
                     ARTLRQFLMNS"
BASE COUNT         1850 a         3697 c         3859 g         2094 t
ORIGIN      
        1 gttgccgcgc ccttgcccgc ggcccttagg cacaaacggg gtgcggaact cgatgtgcag
       61 cgagtggtcg ccaaaggcct tcttgctgac gcagccagtg gcccccaaca gattgtcttc
      121 gatgatcttg ccgttctcaa agctgtcggc cgtgctgccg tcgaacagca ccagcgcgcc
      181 ggctggcggc ttggcgccga gcgtcgggct tttgcgctcg acctttttca gctcgccgat
      241 cgtcgcgccg tcctgcatga cggtgaagac gccgtccttg atcgtggcgg tccaggtgtc
      301 gccttgcacc tgcaccgtgc cgtccttagt ctcacccttg ccgcggcgag tttgatcgcc
      361 ccgcttccag ccgtcgccag gcaggccgcc gcggaaaccg acgacgtcga acttgccgtc
      421 gcccagtgcg atcacctgcg ccccccacac ctgatcgccc gccccgccct tgtactcgcc
      481 ctgaaccgcg tagtccggcc cggcgtcctt ggcattgctg aaggcctcct cagccgcgag
      541 cgaggcagtg gccagacagg cgcagagcgc gacactggcg aatcgaacga acatgtcgaa
      601 atctcccgtt gcgttggacc cgttacgtga atcaccaata ccctagccgc ccgccgcggc
      661 gaggaaaata ggctggcttg ctcctggatt gcgcatctta atccttctcc tgctcttgct
      721 cttaatcctc ttcctaatcc tactcttcct cctaatcttt ctcctgccgg ccgcggccga
      781 acggggagga gaaagagaaa gagcaagatt aggtgacctt ccccacgaac cgtggggaag
      841 gtcataggag gcaaatgcgg cctcgctccg caaccgggtt ttttcctaca atcgccgcat
      901 gagcttcgct ggatttcttg ccgccctgct ggagcacgga cgcgtcaagg tcgcgcatcc
      961 gcaggagcat gtgccgtcgg atgagatcgc ggcggccggg cggcagttcc aggagcgggc
     1021 cgccctcgtg gcggcggagt ttcccggcca gccgccggca ctggatgttg cggtggcgca
     1081 gtgggcggca ctgcagatgt accgcgcgtg ccagctggcg atttaccgcg aactggatgc
     1141 cggcgcgatc gacgagctgc tcgcgcccgc ttgtccgccg gccgagccgg ccagccggca
     1201 ctggtcggcc gatctggcgt ttgtcttcct gcccgacctc atccgccagg cgcaggcagc
     1261 tgcggcgaac gacccactcg taactcgctt acgcgagtgg gcggcggact ggccgctgtc
     1321 gagcgtcggt gtggcgcagg tcgagccgcg aaatgtgacg gagatcgccg gccatccggg
     1381 attactgcgg ctgtacgtcg atcgcatttt gagcaagaag gattggccgt gactgtccga
     1441 tcccgcagtt cgcgccgcgg cccaggagtc gattggcgct tttgtttccc agtggccgga
     1501 agcggccaag cactttcaga cagccgcccc atgaccgatc cccgcccgca cctggcccgc
     1561 cggctggcga ccgacgttct ggcgtcgctc aaaaacgcgt tcgtcggcaa ggatgagatc
     1621 atcgacctga tgggcgtttg cctggccggc ggcgagaacc tcttcttgct tgggccccca
     1681 gggacggcca agagcgccct ggtgcagcag ctcgcgcggc ggatcgacgg ccgcgtgttc
     1741 gattacctgc tcacgcggtt taccgagccc aacgagctct ttggcccgtt cgacatccgc
     1801 aagctccgcg aaggggagct gatcacgaac accgaaggca tgctgcccga ggcggcggtg
     1861 gtgtttctcg acgagctact caacgctaac agcgcgatcc tcaacagcct cttgatggtg
     1921 ctcaacgagc gggtcttccg ccgcggccgc gagacccgca gcctgcagct gctcatggtc
     1981 gtcggtgcca gcaaccgcct gccggaggac gacgcgctgg cggccctctt cgaccgcttc
     2041 ctgcttcgcg tgcggtgcga caacgtcccc gaggaccgcc tgggtgaagt gctcaccgcc
     2101 ggctggaagc ttgaccgcgg cctgaccggc gagcacgcca cgctgtcggt cgaagaaatc
     2161 gcggcggtgc agcagctcgt gtttgaggtg gacctttcgc cgatccagcg gccgtacgtc
     2221 gagctggtcc acaagctgcg ccaggcggga attcccgtct cggaccgccg cgcggtgaag
     2281 ctgcagcggc tgatggcggc cagcgccgcg ttgtgcggcc ggaccgccgc cgacacgacc
     2341 gacctctgga ttttgcggta catctgggac gtcgaggagc agcgcgaagt gatcgcggcg
     2401 atcgtcaacc aggccgtcga ggccagcccg cccgaggccc aggccacgag ccatccgcga
     2461 gccaaacagt cgtccggccc cgatcccgaa gagctcgccc gcgacctcgt gcggattggc
     2521 cagcggctgg ctgatccggc gctcgcggcc gccgaacggg cggtgctgcg cgatcagctc
     2581 ggcttgctgg ccggccgctg ccagtgggtc gtcagccggc agcagcgcga cttcctcgac
     2641 cagcaggtcg ccacgctgtg gacgcagttc ggggtgcgac cgtgagcggc acctgggcgc
     2701 tggccgcgcc gcgcgagtcc ctgccggcga tcgcccgcct gcggcaagta ccgggactgg
     2761 cggtctgcga agaaagtcag cggctgtggc tgcggggtcc ggagctcgac gatgcgctgg
     2821 ccgccgcgct gaaactaatt cccggcggct gccagttcgc ggtccttcaa gacggccagc
     2881 tcatcgccgc cggcaagctc gtccccagcg gccacctgcc cgcgggcccc tggcaacttt
     2941 tggccgattg gctgacgctg gagctgccgc cgctgcaacc accgggcgtg taggcccctt
     3001 ctgccggatg ctcacccggc ggcatcctcg tcagcggcgg gcgcgttcag caggcggacg
     3061 gcgacgccac catcgccgcc cggctcgaat tgatagatgc ggtcggcgta gcgctcgaac
     3121 agggccacgt cgtggtggct gatcatcagc acttgaaaac cgagggcccg cccggcttcg
     3181 tgcacgatct tcaccagccg cggaaccagg tcgggccgca gccagcagtc ctgctcgtcg
     3241 aggaccagga atcggcggtg cctgctgggg tcgagcgtgg ccagggcgaa cattcgcagg
     3301 ccgacgctga ggatgttggc caccgagccg ccctggccgc ggaggatgtc ctccttgtcg
     3361 ccgccgcgct cgacccagaa ttcgaccgtc gcctggccac gcttgaaatc ggccgccgcc
     3421 ttgaactcga tcggctgctc caggatttcc cgcagggcga tcgtgagctt ctcctgcacc
     3481 gtgaccagca gctgctggaa gagctggtcg ctgagctgct ccagggccac cgtcacgtcg
     3541 ccggcgatat cgaggtacgc gttcagctcg gtgaattttt tggcgagatc gatggagcgc
     3601 tgttcgcgct cctcgcggct gtgcagcagt cggtcgactc gccggcgggc gtcctgaggc
     3661 gtgcgcagcg tggcggtatc ggcggtaccc atcatgccgg cttctccgcc gactttgtct
     3721 cggcaaactt ctgctcgacg gcagccaggt cggcctcgat tctctcgagt tcctgctggt
     3781 agccggcccg agcggcttcg ttctcggact gcatctggcc gagcttcttc cgcagctcgg
     3841 ccaggtcgtc ggtcccgtac ttttcgcggg cctccttccg cagctgctcc agctggttgc
     3901 gggcgtgcaa caggttcgtg tcggcctgga tcttcttcgt gtgcagccgg gcataccgct
     3961 cctgaagctc ttcgatgctc tgctgcccgt tgctcgccgc cttattcgcc atcgctcagg
     4021 acctcgcttg ccaggttcat gatctcggcc gccaccgctg gcgggaattg cgccaggttc
     4081 tgctccagga acggccgcaa gcccgctcca ctgcctgttc gccgcgcttg cagctcggcc
     4141 aaacctgcca cgaacgccga gccggcggct gaggccggca gatcggcgac ggcctcgtgg
     4201 aatacgtcgt cgaacggccg gtgcgggacc tcgacgaacg tcaacttgta gctcgccggc
     4261 gacacgtcaa tccgcagcgc ggccggcacg tgcattcgcg tggcttcgtt ccgcgagcgg
     4321 cgagtgatgt tgcccggcgt cagccagcgc gtcgctccga cccgcacttc ctccagccgg
     4381 cggtgaatgt ggccgttgat cagcagctca acgccctgaa tctcgcaggg ctggatgcgg
     4441 ccctcgtcgt agcctggaat tagcacgtcg tgatgcgtca gccaaatgac gagcggctgg
     4501 cgccccgcgg cggcaggcgg ctcaaaaacc ttgggaatcg gctgccggta ggacgatccg
     4561 ccgatgaccg cctcccgccc gcccagcgtg ccgatccagg gatgctcggc gtcgatcaga
     4621 cgcaggcgcc ccgacttcac caggaggctg atagaatcgt cgtcggatag ctgcgggttg
     4681 gcgcagtcgt ggttgccgaa caggccgatg acttcccccc gcagcaggtc gagcagtcgg
     4741 gcgatcagcc agttggggtt gtcgcgcggc ttgtcgaaca ggtcgcccag gatcgccggc
     4801 aagaggcgat gggccgcggc ataatcgagg caccaggcga gtttttccag aatgacgctg
     4861 ggataatcgt ccttgcgaaa tcccggctgc cggccctcca ggtgcggatc gccgatgacg
     4921 agcaggccgg tgtaagcgcc ttctggctcc ctctcccctt gggagagggt cagggtgagg
     4981 gtgttttgtt tgtagcttgg catttctaga ccctcaccgc ggccctctgt agggaacgcc
     5041 ctctgtggcg ttccggttcc cggtcgccgc gtgggccagt aaccgctcgg ggtcgagcgg
     5101 cacgccgcac gttgggcaga cctcgccgtc cgcggcggca cggagttccg tcgcggcggc
     5161 attcagctca gccaaggcag cggcttcttc gcgggcggac tcggcaacga gagcgagcgc
     5221 ggcttcgaat ttgccggcga agtcgcgaag cggagcaagt tccagctgcg gccgcggcgc
     5281 ttcgattcca gcgactgatg cggccgattc ccatcgcatc gcttgggcgg cggcagcgaa
     5341 gaggttctcg atcagcttcg ccagcgccgc ggaatcggcc aaccgcggcg gctcgcagat
     5401 ggcagacaat cgctggcgct cggcctcggc ccgcgcggcc gcccggtcca gcgccgcgag
     5461 ctcgtccgcg gtgcgggcca gcgcggcgac gtccgacatc gccggcggcg cggcgagcgg
     5521 cgctagctgc tgcgctcggg cggcggcttg ttcgtgcgcg gcggcggcgc gctggagggc
     5581 gtcgatcaag tcggccagcg gctgcgtcgg cggtaatttc ggcggcgggg cgagcggctc
     5641 gaggctcgct gcgagggcgg ccacttgcgc cgcctcggct tgctttgcgt ccagcgctgc
     5701 ggcggcccgc tgggcctgct ccagctcgcc gctgcggcgg agcagctcgg cgtgctgctg
     5761 ctcggcggcc ttgagccgct tgtccaggtc gaccgctggc tgaagagttt ccagctcggc
     5821 ggtgagctgc ttcgactcgg cctccaggcg gttcttttct cgctgccgct ccgccaggcg
     5881 agccttgtgc cggctttgca tctcgaccag ccggctggcg tccgacgagg aagcgaagaa
     5941 gcgggcggcg gtggccgagg agctgttcag caagaagatg ggggacttct gcgtgccgaa
     6001 gtgcacgtcg aagtcggctt caccgctgcc ttcgaccagc ggcaagcgga gggcacggtg
     6061 caggggctcc gaaacgtccg cgccgcggag gcggtcgaac ttttcgccgt cgatctcgta
     6121 gctcgcgccc gtttttttcc gcttccaaag ggccgtgtgc ccgtcgtccg tcgtcacctg
     6181 aacggagcat tccttttcgc cgtgccgcag gacgtaagtg gaggggtcgt tgtggcagag
     6241 aatctgcaga gccgctacga cggcgctctt gccgcaattg tttggaccga cgagcacggt
     6301 cagccccgcc gccggctcga tgaccgtgtg ggcgtgggcc atgaagttcg tcagctcgat
     6361 gcgggtcagc atcaggagtc aggggtcagg agtcagtagg tgtgaacagt ggaccaacgt
     6421 gcattgtggg gggagtgcgg cgttttgtca gcgggaggcg gacacaggct gtccgtgctg
     6481 ccagcgttgg cggtgctcgt tcacggcgcg gcgggcgcgt gttacgatgg ttgccctgcc
     6541 cctccctacc ttcccggccg ccggagttcc tgatgtccag ccccgtctcc accgcaaaac
     6601 ccctgacgtt tacgcagtgg atgatctgca cgatggcggc gatcggcttc gcgttcgaca
     6661 tctacgagct gctgatgctg ccgctgatta tccggccgag cctgctggag ttgaccggcg
     6721 ctacgcccgg cacgccgacg ttcaactatt ggggcgggat gctgttctat gtgccggccg
     6781 tggccggcgg catttttggc ctgatgggcg gttatttgac tgaccgcctg gggcgccgcc
     6841 gcgtgctcac ctggagcatc ctgctgttcg ccttttccaa gttcttcgcc ggcttctcga
     6901 cgagtgtttg ggtcctctta atcctccgct cgacggcgtt cgtgggcgtg tgcgtcgagt
     6961 tcgtcgcagc ggtggcttgg gtcgcggagt tgtttcccga tccgaagcag cgcgagaaag
     7021 ccctgggcta tacgcaggcg ttctcgtcgc tcggcggctt gatgatcgca attgccaacg
     7081 gcctgtgcat ccagttcgcc aatcagctgc cgccgatctt cattcccgaa gcgttgacgg
     7141 gcattttcgg gacgatcgag gtggctcacc agcacgagcc gtggcgctac acgctgatct
     7201 cgggcctgat tcccgccatt ccgttaatca tcatccggcc gttcttgccc gagtcgccgg
     7261 tgtgggcgga aaagcggaag gcgggaacgc tcaagcggcc cacgattctg gctctgttct
     7321 cgcccgagct ggcccgcacg tcgatcgtca cggccatcgg attcgcctgc acgctgggca
     7381 ttgccttcgg cgcgatccag cagatgccgc agatcgtgcc gggcctgccg gaagtgcaag
     7441 cgggcgcgaa gaaagccagc gacgcggcgg tcgccaaagc caaagcgaag ggcgaggcca
     7501 acgccgacac gctggccgca gtcgccagag gcgtcggagg agcgtaccgc cagaagatcg
     7561 cctcggaata tacgaagatg caagaagtgg gcgggctgat cggccggttc ttgatcgcat
     7621 tggtcctcgc ggccggcgtt ttgcgttggg gcaacgcgct gcggttgttt cagcttcccg
     7681 gcttgatttt ggtcccactg gtgttctggt tcttcctccg tgcgccgaac cggactttct
     7741 tcgagattcc gctggaggcc gtgtatctgg ggaccgtgcc cgtaacgacg atgtcggtcg
     7801 gcatgttcct ggtggggatg ttcgtcgtcg cgcagctcag cttctggggc aactatctgc
     7861 cgcacgtgta ccccattcac ctccgcggga cgggtgagag ctttgccgcc aatatcggcg
     7921 gccgcctgat cggcacgttc gcggccatgc tcacctggtc gctgtcgggg ctgctccctt
     7981 ataaggacgg accgttgcag ttctcaacgg ccgcagccat cgtcggcacg acgattgccg
     8041 tcatcggctt cctcgccagt ttctggctgc cggagccgca tcccaagggc ggagacgagt
     8101 agcggcgtcg gcttcgagtt acaacccaaa ccgccggctg aagcggcggc tgcggtggag
     8161 ggcgtaccag cggcgacggg cggcgttgcg gctgaagcgg cgtttgcgtc gtccgtccgg
     8221 catggctgac tcctcccatt gagtcagacg cctacaaggt cgcctccgtc aacagcggat
     8281 aacgaggcag ccgcgggccg cactgggaat agggattcac caggcggagc gggcgcgtca
     8341 tccagatcac ggggaccagc acaatcccga gtgccattgc cagcatcagg aggtcggcaa
     8401 gcatctgagt ttctttctta agcgtgcgaa acggggtcgc tgctactcaa gaaatgcaac
     8461 gagcgtgccg agcggccaaa accgccggga attcagcggt tgaagatcgt tctgacaaac
     8521 tttgcgggcg cgcctggtgc cgccctgtca tcgaattgac gatgccagcg tcgcggggcg
     8581 gacaaactca aagaaccgaa gaaccaaacg gcagcccttt ggaatttcgg tcctttggtt
     8641 ctttgatttt tctgcacttc taccacggtc cacggaccgg atagacgccg aactggtcgg
     8701 tgtggctggg ccagtagggc gtggcgtaga tctccgcgcc gccgccgacg gccagttcgc
     8761 cgggatagtc gcggaagaac tggtgataga tgggaatcat ctgcgactgg ccgacgcccc
     8821 aggcgtagct ggtgtgcatc ttcgagttcg gcggcacgac cagcgccgtc ggcatgcccc
     8881 acggcgtgta ggcccattgc ccgtgccagg gccgctgcga ggcgtagtag cgagccatca
     8941 cgtcggcccg gatcgcgtga tagccgtgat gcggatgaac cgccagcgcc ggcgtcgccg
     9001 cagccgcaat caagaggccg gcgagcaacg agaggatggt aagggatttc acggggggga
     9061 actccgcttg gttcttggtt cttcggtttg gttctttggc cttagtagcc gatctgcccg
     9121 aacggcgacc aggtccaatg caccttggtc cggttcaggc cctggccgcc gttgtagtac
     9181 tggtggtacg tgcggtgatg cgtgtacagg aactcgtgcg gatagagcgg ctgataggtg
     9241 atgtaggtgt ggccgacgtg cggcggcacg ggacggggcg agaggtagag ctcggccccc
     9301 aggcccgtgc agctcggcca ggcgtagtag ttgtagaaga ggtccggcgc tccgtagaac
     9361 cggtactggc agttgccgtt gttgcaggtg cggcagccgc cggggccggc ccacgcgcgg
     9421 gcgtaggcgc ggtcgctgta gttagtcacg ccgtcgggat agccgctcat gatcgacagc
     9481 ggagcggcat cgacgcggcc atggcggccg aatacgccgc cgtgaccgcc attggcgccg
     9541 tagccgtcgg ggcagctgcc gtcggactgc acgtcggcgc cggcgtaata ctcggaattc
     9601 gcgggggtct cgatcttctg ggcgtgcgcc gctgaggcca gcagcgcgag gagagcggcc
     9661 gagccaagga tggaacgcag catgaacaac tcctgcggat aagctccaag aaccgaagag
     9721 ccaaaaaacc aaagacgccg cctacttttg gttcttaggt tcttaggttc ttaggttctt
     9781 aggttcttgg ttttttgact cctttccgtt ttctcggaaa gcgccgcgcg tgcgggtgag
     9841 tctttatgcg cctttggcgc cagttgcccg gcgcgtgccg gcagcggcgt tagcaccgtc
     9901 aaacccgcct gggactgttg cgggaaaaac ggtataaaac gtacagtttt acaggtgggc
     9961 ggaagatcgt gccgtcaggg agggcgaggc tcctgccgag ccttttcgcg gccgcggctc
    10021 ggcggaagca tcgccctccc attgctgctc gaggtaaagg aatgcccctt tgactgccgt
    10081 gaaaactcga tcctctcccg gccagaagtc gcttcctttg gatgccgctg cgaacgccac
    10141 caggtcgaac ggcgcggcca atggccagcc gcacgccgat gcacaaagcc cggcggccca
    10201 cgaagaagtc gatcatggcc gcggcaaacg gcgggcgacc gccgagtcga tggcggccaa
    10261 acaacgggac atctcggtca gcgagttttt tgccaagaac cgccacctgc tggggttcga
    10321 caatccgcgg aaggccctgc tgacgacggt caaggaagcc gtcgataact cgctcgacgc
    10381 ctgcgaagag gcggggatcg tcccggagat ctgggtcacg atcgaagcga ccggcaacaa
    10441 ccgcttcaag gtcagcgtgc aggacaacgg gccgggcatc gtcaagaagc aaatcccgct
    10501 gatcttcggc aagctgctct acggcagcaa gttccaccgc ctgcggatga gccgcggtca
    10561 gcagggcatc ggcatctccg ccgccggcat gtacggcatg ctcacgacgg gcaagccggt
    10621 gaagatcatc agtaagatct cgcccaaaaa gccggcccac tatttcgagc tgcagatcga
    10681 caccaagcga aaccacccgg agatcatcaa cggcaagggg gaaggggtcg atttttcgcc
    10741 cggcgagaaa gggcatcagg agatgctcaa gcagggcgtc gattgggtca gccattaccc
    10801 cggcgcccag gggaaggagc ctcaagaggt caaaagcggc acgcgagtga cgatcgagct
    10861 ggaggggcgg ttcatccgcg gccgcggctc cgtcgatgaa tacctggagc agacggcgat
    10921 cgccaacccg cacgtcacgc tgcactacaa agacgccgaa ggcaacgagt cgagctatcg
    10981 gcgctccacg acgcaattgc cgccagagcc gaaggagatc aagccgcatc cgtacggcat
    11041 cgagctcggc cggctggtga cgatgctcaa ggactcgacg gcggggacga tgtcggcgtt
    11101 tttgactgaa tcgttttcgc gggtcagctc aggggttgca cggaagattt gcgaagaggc
    11161 caagatcacc acgcggtcga acccgcggcg gatcggccgg caggaggccg attcgctcta
    11221 ccaggcgatc cagaccacgc ggatcagctc gccggcgacc gactgcctcg tgccgatcgg
    11281 cgagcagctc atcctgaagg gcctgcacca ggtcgtgccg ggcgagtttt actgtgcggc
    11341 gacgcggccg ccggccgttt atcgtggcaa cccgttcttg atcgaggtcg ggctggcata
    11401 cggcggcgtc tcgacggcgc aaaaagtgcc gctcgacacg ctgaccgaga tgctgggcga
    11461 aacggatgcc cggacgctgc ggcagttttt gatgaactcg
//
LOCUS       DASRJB010000149         7532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2580065, whole genome shotgun sequence.
ACCESSION   DASRJB010000149 DASRJB010000000
VERSION     DASRJB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580065"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFB80_04270"
     CDS             complement(<1..842)
                     /locus_tag="VFB80_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33005.1"
                     /translation="MPRLLATCLVGLTAALALVATASGETNWPRWRGPQQNGHTTETN
                     LPVNWSAQNIAWKTQLPGVGQSSPIIWGDRIFLTAELGKGQERLVFCVDRQSGKILWQ
                     QTAWKGTPEKSHDMNGWASASCATDGEIVVAFFGVGGIHAYTVEGKPLWSKDLGTFAG
                     PWGTAACPVIVDQLVIQNCDADTNANLIAFNKKTGQEAWRTKRKDYRGWSTPIVVDAG
                     KRRELVLNGHEGVQAYDPATGRELWFCSSPVGRGEPTVTPVGDLLCVVNGLKGGNFYA
                     VRPGG"
     gene            1274..2566
                     /locus_tag="VFB80_04275"
     CDS             1274..2566
                     /locus_tag="VFB80_04275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN33006.1"
                     /translation="MSFSELQLSDPLRRAVESAGYHTATPIQLEAIPPLLAGRDLVGC
                     AQTGTGKTAAFALPTLQRLQQLGKNQPGQPRSRHIRTLVLAPTRELATQIGESFRTYG
                     RFTGLRHTVIFGGVGQNPQVKALAHGVEIVVATPGRLLDLMNQGFVDLSRVEILILDE
                     ADQMLDMGFIRDLRRIVSRVPRQRQTLLFSATMPAEIRGLADEWLRDPVSVSVVPHST
                     PAERIEQSVYHVEKRAKPQLLAQYLREQAARRTLVFTRTKHGADKLVKSLVQSGIRAE
                     AIHGNKSQNARQRALAQFKSQHPPVLVATDVAARGLDIDEVSHVVNFDLPMTPELYVH
                     RIGRTARAGAAGIAVTFCDREERGMLREIERLTRQRLPVAGGNAPPAEADLEPAGQSQ
                     QPRRFQHSPQQHRSGRQGQRPFRGFQGKPKSGRRFSRA"
     gene            2612..4594
                     /locus_tag="VFB80_04280"
     CDS             2612..4594
                     /locus_tag="VFB80_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33007.1"
                     /translation="MTGSIGTGHRGMAASNPPPKIACPHCQALIKSPALPTGSQVNCP
                     KCGQGFRLGQEAGGGSRESGVRGQAPEARSQEPGGRGQIAGAGVPTQTPRPAPAAAAP
                     SRPPRKLDNSVDPKVAPLNPGNVAPTRPLAPLPPLEDDGFVPLDGSEPKRGQQPAGKR
                     PPPVPAEVSAAALHAAAGVVPLPQAPRTEPIDPNLLPPPPPRKKEKVTHVPVVCRLCG
                     TRMHAPLEKIGQTLQCPDCHTVNEIKPLKVREEKKIPKGPSLDNAEEFGLDVTVERPA
                     YRPLQAPRGDYAILAAMDPDSAPPGWQPPDPRAPPVAAAASAAPAARASAPVAEAEPA
                     GTSEAEPAPESAAPEYGEVEEEEDFVISEPVERIEYKPPPPRLPPPEPNAEMYDGRYT
                     DDHFGTEIDRGAAGAWRRAPFRVGVIEFLFHRDALLRWILYGMGAAALVALANAARPS
                     ETDDLVAKALEQGQQYVYAFLFGLGFMAWVAPFSCCLFAIVEDTANGSDQVTSWPDYN
                     ILEWFLKASYFPVAGIVAGFPGLVFGSFIVSTGAAPVFALPLVILASWVFLFPLVLSS
                     MLAESSVVAFYSPSTYRSLSVAADSWLIFYLLQILLGFLAALALVLAQVRFFPVSSVG
                     AAALVVLAFLFCRLMGRMMWIAQEKLAKAPAAESED"
     gene            complement(4591..7056)
                     /locus_tag="VFB80_04285"
     CDS             complement(4591..7056)
                     /locus_tag="VFB80_04285"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HZN33008.1"
                     /translation="MAQFAQRRLLSRMAAAARELAGEWSGRDFGGASPDTVAQAAVCA
                     APILASGRNDHAALNWLCRQLAPWESFALEQALRPSLAPSLPLSPSPSHLYDAFLHHH
                     ARDRRKRHGVFFTPQPIADYIVRRIDAALRNDFGLPGGLADDSAFRTPHSAFLLDPAC
                     GTGVFLLAVIDFLHSRLADCWNELVPALLPRLIGIELLPAAAFLAKLNIALQLAETGY
                     DFRQPGSIQIHTADALDPNLQFAICNLQSAIPVLLGNPPFSSLSTNTNDWIAGLVRGD
                     GQTRGYLRAGDERLGERKTWLHDDYVKFIRLAQWHVEQAGRGIVGLVTSSGYLSNATF
                     RLMRQELLRVFPRIEILDLHGSRKAHEVAPDGGPDENVFGLAQGVAIGIFSRPATPTR
                     SVSEGLPEHRLTSGDLWGSRDAKLAALSNGHPVTTATAALAPPDFRFTSSPHHAGHPE
                     YAAAWSLAEAMPLNATAPVTARDHFVVAFTQDELRQRVAEFRDLAIPDDEIRRRYFQR
                     TRSARYETGDTRGWKLAAARRAVAADADWETRIVRCLYRPFDWRHVFWHPAMIDWPRA
                     EVTQHLLLQNGEPNGIYEWARGRGGVRERALLNAPSLPLSPSPPLVLIARRQQLPTQP
                     CTFFWIADGLALDGVIRSDNRGSESLFPLYVRELSGWRANFAPQFVAQAERAADLGYI
                     PLGCGDLARAFGPEDLLAYIYALFHAPTYRARYAAALVTGFPRVLLAGGECFRTLARC
                     GRELIDLHLLRTTFASNGEPASLPQVAGFRAGGYVALRKWLQPRHRSPADPQYGQIAA
                     AIARTLELMGEIDGALGLPQPGA"
     gene            complement(7059..>7532)
                     /locus_tag="VFB80_04290"
     CDS             complement(7059..>7532)
                     /locus_tag="VFB80_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZN33009.1"
                     /translation="FIVGFCGETEADFQQTYELVKESRFKNSFIFKYSERPGTKGAEL
                     YADDVPFEVKNRRNNELLELQNRLSEQDNQQFLGRRVEVLVEGASDRAKSRGEDAGES
                     VQLMGRTPDDRIVVFDGQPRLIGQIIPLAIYDVAAHTLFGAVVTEHVGPPLSVLA"
BASE COUNT         1220 a         2472 c         2615 g         1225 t
ORIGIN      
        1 ccgcctggcc gcacggcgta gaaattgccc cccttgagcc cgtttacgac gcacagcaag
       61 tcgccgacgg gcgtcaccgt cggctcgccg cggcccaccg gactgctgca gaaccacagc
      121 tcgcggccgg tagccgggtc gtaggcctgc acgccttcgt gcccgttgag gaccagctcg
      181 cggcgcttgc ccgcatcgac gacgatcggt gtgctccagc cgcgatagtc cttgcgcttc
      241 gtccgccagg cttcctggcc ggtcttcttg ttgaaggcga tcagattagc gttcgtgtcg
      301 gcgtcgcagt tttgaatcac gagttgatcg acgatcacgg ggcaggccgc ggtgccccac
      361 ggacccgcga acgtgcccag gtccttcgac cagagcggct tgccttccac ggtgtaagcg
      421 tgaatgccgc ccaccccgaa gaacgccacg acgatctcgc cgtcggttgc gcaggaggcc
      481 gaggcccagc cgttcatgtc gtgactcttt tccggcgtcc ctttccaggc cgtctgctgc
      541 cagagaatct tgccgctctg gcgatcgacg cagaacacga gccgctcctg gcccttgccc
      601 agctcggcgg tcaggaaaat ccggtcgccc cagatgatcg gcgacgattg gccgacgccg
      661 ggcagctgcg ttttccaggc aatgttctgg gccgaccaat tcaccggcag attcgtctcg
      721 gtggtgtgcc cgttctgctg cgggccgcgc cagcggggcc agttggtttc gccgctcgcg
      781 gtggcgacca gggccagggc agcggtcagt ccgacgaggc aagtggcaag caagcgcggc
      841 atgggtcggt cctggaaagg cgggtttctg aggcgggata cggacaatat ggcccaaagg
      901 cagggctctc acaaccgtat ttgcccttga cgggcttcct gctggtgcta cccttgagag
      961 ctgaatgaag gtgcgagacc agtttgagtt tgctccatcg agttgactgg actgtggtca
     1021 cttgcttcgt ctgttagggg ctgaggcgct caatcagcag cttcggcatc tgcccattcg
     1081 accgcgattg atgatcttgc tcctaggcgc tgttccgctg aagcgcgaac gttttagcgc
     1141 cggatgagaa gacgacggtc gtctttttgt agccgaattc gcgagaattc gggcccgcgg
     1201 gtgcatccga attctggtga attcggctac ggcaccgatt cacgtctttg cacaaggttc
     1261 gtaaaaggtt ctcatgagtt tctccgaatt gcaactgtcc gatcccctcc gccgcgccgt
     1321 ggaaagcgcc ggctaccaca cggccacgcc gattcagctc gaggcgatcc cgccgctgct
     1381 ggccggccgc gacctcgtcg gctgcgccca gaccggcacc ggcaagacgg ccgcgttcgc
     1441 cctgcccacg ctccagcgcc tgcagcagct gggcaaaaat caacccggcc agccgcgcag
     1501 ccgccacatc cgcacgctcg tcctcgcccc gacccgcgaa ctggccacgc agatcggcga
     1561 gagcttccgc acttacggcc gcttcaccgg tttgcggcac accgtcatct tcggcggcgt
     1621 cggacagaac ccgcaggtga aggcgctcgc gcacggcgtc gagatcgtcg tggccacgcc
     1681 cggccggctg ctcgacctga tgaaccaggg cttcgtcgat ctgtcgcgcg tcgaaatcct
     1741 gatcctcgac gaagccgacc agatgctcga catgggcttc atccgcgatc tgcggcggat
     1801 cgtcagccgc gtgccccgcc agcggcagac gctgctgttc tcggccacga tgccggcgga
     1861 aatccgcggg ctggccgacg agtggctgcg cgatccggtg agcgtcagcg tggtgccgca
     1921 ttcgacgccc gccgaacgga tcgagcagag cgtctatcac gtcgagaagc gggccaagcc
     1981 gcagctcttg gcccagtacc tgcgcgagca agccgcccgg cggacgctcg tcttcacccg
     2041 caccaagcac ggggccgaca agctggtcaa gagcctcgtg cagtccggca tccgggccga
     2101 ggcgatccac ggcaacaaga gccagaacgc ccgccagcgg gccctcgcgc aattcaagtc
     2161 gcagcatccg ccggtgctcg tggcgaccga cgtcgccgcc cgtggcctgg acatcgacga
     2221 agtgtcgcac gttgtcaatt tcgacctgcc gatgacgccc gaactctatg tccaccgcat
     2281 cggccgcacc gcgcgggccg gggccgcggg catcgccgtc accttctgcg accgcgagga
     2341 gcgcggcatg ctgcgcgaga tcgagcggct gacgcggcag cgcttgcccg tcgctggcgg
     2401 aaacgctccg ccggccgagg cggacctcga acccgccggc cagtcgcagc agccgcggcg
     2461 atttcagcac tccccgcagc aacatcgatc cggccggcaa ggccagcggc cattccgcgg
     2521 ctttcagggc aagcccaaga gcgggaggcg attctcgcgc gcctagccaa tagttcgtct
     2581 cgcggggcga atactggcgg ccggtgatac aatgacgggg tccatcggaa cggggcaccg
     2641 gggaatggcc gccagcaatc cgccgccgaa aatcgcctgc ccgcactgcc aggcgctgat
     2701 caagtcgccg gcgttgccca cggggtcgca ggtgaattgc cccaagtgcg gccagggctt
     2761 tcggttggga caggaggcgg gaggcgggag tcgggagtcg ggagtcaggg gccaggcgcc
     2821 agaggccagg agccaggagc caggaggccg gggccagatt gcaggtgcgg gcgtaccaac
     2881 acagacgccg cggcctgctc ctgctgctgc cgctccaagc cggccgccgc ggaagctgga
     2941 caactcggtc gatcccaagg tcgcgccgct caatccgggg aacgtcgcgc cgaccaggcc
     3001 gctggcgccc ttgccgccgc tggaagacga cggcttcgtg ccgctcgacg gcagtgagcc
     3061 gaaaagagga cagcagcccg cgggcaagcg cccgccgccg gtgcctgccg aggtttccgc
     3121 cgcggcattg cacgccgcgg cgggcgtcgt ccccctgccg caggcgcctc gaaccgagcc
     3181 gatcgatccg aacctgctgc ctccgccgcc gccgcggaag aaggagaagg tgacgcacgt
     3241 gccggtcgtc tgccggctgt gcggcacgcg gatgcacgcc ccgctggaga agatcggcca
     3301 gacgctgcag tgtcccgact gccacacggt gaacgagatc aagccgctga aggtccgcga
     3361 ggagaagaag attcccaagg ggccgagcct ggacaacgcc gaggaattcg ggctggacgt
     3421 caccgtcgag cggccggcct atcgcccgct ccaggccccg cgcggcgact acgcgattct
     3481 ggccgcgatg gaccccgata gcgcaccgcc cggctggcag ccgcccgatc cgcgtgctcc
     3541 gccggtggcc gcggccgcca gcgcagcgcc cgcggcccgc gcgagcgccc cggtcgcgga
     3601 agcggagccc gcgggcacaa gtgaagcgga gcccgccccc gaatccgcgg cccccgaata
     3661 tggcgaggtg gaggaggaag aggattttgt catctccgag ccggtggagc ggatcgagta
     3721 caagccgccg ccgccgcggc tgccgccgcc ggagccgaac gcggagatgt acgacggccg
     3781 ctacaccgac gaccatttcg gcaccgagat cgaccgcggt gcggccgggg cctggcggcg
     3841 agccccgttc cgggtcggcg tgatcgagtt tctgttccac cgggacgcgc tgctccgctg
     3901 gattctgtac ggcatgggtg cggccgcgct cgtcgccctg gccaatgccg ccagaccgag
     3961 cgagaccgac gacctcgtcg ccaaggcgct cgagcagggg cagcagtacg tctatgcgtt
     4021 tctgttcggt ctgggcttca tggcctgggt cgcgccgttc tcgtgctgtc tgttcgcgat
     4081 cgtcgaggac acggcgaacg gctcggacca ggtgacgagc tggccggact acaacatcct
     4141 ggagtggttc ctcaaggcgt cgtattttcc ggtcgcgggg atcgtcgccg ggtttccagg
     4201 gctggtcttc ggctcgttca tcgtctccac cggcgccgcg ccggtatttg ccctgccgct
     4261 ggtgatcctg gcgagctggg tcttcctgtt tccgctggtg ctctcctcga tgctcgcgga
     4321 aagctcggtc gtcgccttct actcgccctc gacctatcgc agcctgagcg tggcggccga
     4381 cagctggctg atcttctatc tgctgcagat cctgctcggc tttctggccg cgctggcgct
     4441 ggtcctggcg caggtccggt tctttcccgt cagctcggtg ggcgcggcgg cgctggtcgt
     4501 gctggccttc ctgttctgcc gcctgatggg ccgcatgatg tggatcgctc aggagaagct
     4561 ggccaaggcg ccggccgcgg agtcggagga ttaagcgccg ggctgcggaa gccccagcgc
     4621 gccatcgatc tcgcccatca gctccagcgt tcgcgcaatt gcggcggcga tctggccata
     4681 ctgcggatcg gccggcgagc gatgccgcgg ctgaagccac ttccgcaggg cgacatagcc
     4741 gccggcccga aaccctgcga cctgcggcag cgacgccggc tcgccattgg acgcaaaagt
     4801 agtccgcagc aggtgcaggt cgatcagctc gcggccgcag cgggccagcg tgcgaaagca
     4861 ctcgcccccg gccagcagga cgcgaggaaa gccggtcacc agcgccgccg cgtatcgcgc
     4921 gcgataggtc ggcgcgtgaa acagcgcgta gatatacgcc agcaggtcct cggggccgaa
     4981 cgccctagcc aggtcgccgc agcccagtgg aatgtagccc aggtccgccg cccgctccgc
     5041 ctgtgcgacg aattgcggcg cgaagttggc ccgccagccg ctaagctcgc gcacgtagag
     5101 cggaaacagc gattcgctgc cccggttgtc gctgcggatc acgccgtcga gcgccaagcc
     5161 gtcggcgatc cagaagaacg tgcacggttg cgtgggcaac tgctgccggc gagcgatgag
     5221 cactagaggg ggcgaggggg agagtgggag agagggagcg ttcaatagtg ccctctccct
     5281 cactccccct ctccctctcg cccactcgta aataccattc ggttctccat tttggagaag
     5341 caagtgctgc gttacttccg cccgcggcca gtcgatcatc gcggggtgcc agaagacgtg
     5401 ccgccagtcg aaagggcgat agaggcagcg gacgattcgg gtttcccagt cggcgtcggc
     5461 ggcgacggcg cgacgggcgg cggccagctt ccagccgcgg gtgtcgccgg tttcgtagcg
     5521 agccgaccgc gtccgctgga aatagcggcg gcggatttcg tcgtcgggga tcgccaggtc
     5581 gcggaactcg gcgactcgct gccgcagctc gtcctgagtg aaggcaacga cgaagtggtc
     5641 gcgggccgtc accggcgcgg tcgcgttcag cggcattgcc tcggccagcg accaggcggc
     5701 ggcgtattcg ggatgcccgg cgtggtgcgg cgaggatgtg aagcggaaat cgggcggggc
     5761 gagagcggcg gtggcggtcg ttaccggatg accgttggac agcgcggcga gcttggcgtc
     5821 gcgcgagccc cagaggtcgc cggaggtgag gcggtgttcc ggaagtccct cgcttacgct
     5881 gcgggttggt gtggccgggc gcgaaaaaat gccgatcgcg actccctgcg cgaggccgaa
     5941 gacgttctcg tccgggccgc cgtccggggc gacttcgtgg gccttgcggc tgccgtgcag
     6001 gtcgaggatc tcgatccgcg gaaagacgcg gagcagctcc tgccgcatca ggcggaacgt
     6061 ggcattcgac aggtagccgc tgctcgtgac cagtcccacg attccgcggc cggcctgctc
     6121 gacgtgccac tgggcgagtc ggatgaactt cacgtagtcg tcgtgcagcc aggttttccg
     6181 ctcccccagc cgctcgtcgc cggcccgcag gtagcctctg gtctggccgt cgccgcgaac
     6241 gagtccggcg atccagtcgt tggtgttggt ggagaggctg gagaagggag gattgccgag
     6301 gaggacggga attgcagatt gcaaattgca aattgcaaat tgcaaattgg gatcaagcgc
     6361 gtcggccgtg tggatttgga tgctaccggg ctgtcggaag tcgtagccgg tttcggccag
     6421 ctggagggcg atgttgagtt tggccaggaa agcggcggcg ggaagcagtt cgatgccgat
     6481 cagcctcggc agcaaggcgg ggacgagttc gttccagcag tctgccaggc gggagtggag
     6541 gaagtcgatg acggcgagca ggaagacgcc ggtgccgcag gccggatcga gcagaaatgc
     6601 ggaatgcgga gtgcggaatg cggaatcgtc ggccaaaccg ccgggcaggc cgaagtcgtt
     6661 tcgcagcgcc gcgtcgatgc ggcggacgat gtagtcggcg atcggctgcg gcgtgaagaa
     6721 gacgccgtgc cgtttgcggc ggtcccgagc gtggtggtgg aggaaggcgt cgtagaggtg
     6781 gctgggcgag ggggagagag ggagagaggg agcgagggag ggacggagag cttgctcgag
     6841 ggcaaaactc tcccaaggcg ctagttgcct gcacagccaa ttcaaggctg cgtgatcgtt
     6901 gcgtccggat gccaggatcg gcgcggcaca gactgcggcc tgagcgaccg tgtcgggtga
     6961 agcgccgccg aagtcgcggc cggaccattc gccggccagc tcgcgggccg ccgcggccat
     7021 ccggctgagc aggcggcgct gggcgaattg agccatcgtc aggcgaggac cgacagcggc
     7081 gggccgacgt gctcggtcac caccgcgccg aacagcgtgt gggccgcaac gtcatagatc
     7141 gccagcggaa tgatctggcc gatcagccgc ggctgcccgt cgaacacgac gatccggtcg
     7201 tcgggggtgc ggcccatgag ctgcacgctc tcgcccgcgt cttcgccgcg gctcttggcg
     7261 cggtcgcttg ccccctcgac cagaacttcg acacgccggc cgaggaactg ctggttgtcc
     7321 tgctcgctga ggcggttctg cagctccagc agctcgttgt tgcggcggtt tttgacctcg
     7381 aagggcacgt catcggcgta cagctccgcc cccttcgtgc ccggccgctc gctgtatttg
     7441 aagatgaagc tgttcttgaa ccgcgactct ttaaccagtt cgtaagtctg ctgaaaatcg
     7501 gcctccgtct cgccgcagaa gccgacgatg aa
//
LOCUS       DASRJB010000150         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3991384, whole genome shotgun sequence.
ACCESSION   DASRJB010000150 DASRJB010000000
VERSION     DASRJB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991384"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFB80_04295"
     CDS             <1..386
                     /locus_tag="VFB80_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33010.1"
                     /translation="SQAFVEGLETFRHLKAAGDLAERLRQASLELRRDVVMANEGTAT
                     FIGETLLTGTPDRAAAADLRARYEAICAAALDLESRLREVEQQTSNPAARRLLGRTLD
                     ALQGVKLSAATMIKLLDLIAPPQRD"
     gene            complement(397..1224)
                     /locus_tag="VFB80_04300"
     CDS             complement(397..1224)
                     /locus_tag="VFB80_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33011.1"
                     /translation="MKPFVAVLRGALLLALLAALARAQATPEKQELTRREWTIGDTPR
                     EALLYVPAAAKTTPSPVVFAFHGHGGTMQRAAVMFHYHDVWPEAIVVYMQGLNTPGRL
                     TDPEGKRPGWQNAAGILGDRDLKFFDAVLASLQADYKVDQRRIYSTGHSNGGGFTYLL
                     WRTRGDVFAAMAPSAAAGPGAAWTSRLADLKPKPVMHLAGENDQLVKYEWQQATIEAL
                     RKLNGCEPTGSEWAKGCTLYESKSGTPVVTFIHPGAHNFPPEAPALFVKFFQQHARP"
     gene            complement(1293..>2428)
                     /locus_tag="VFB80_04305"
     CDS             complement(1293..>2428)
                     /locus_tag="VFB80_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HZN33012.1"
                     /translation="GIHLAYKIAQENLIRGGVNRVILCSDGDFNVGTTSTGDLVRLAI
                     EKSRGGVHLSVLGFGIGNHNDAMLEQIANKADGNYAFIDSDREAQKVFVEQMQGTLVT
                     IAKDVKIQVEFNPAEVAAYRLIGYENRRLADRDFNDDTKDAGDIGAGHSVTALYQVVP
                     VGVESELGRPGVDPLKYQETAASGRREPPDGVPLTEAAKSGEMLTLKLRYQPPAGGTS
                     TLLTFPVKDSGQRFGSASPDFQFAAAVASFGQLLRNSKYKGDVSFAAVLETASAAKGQ
                     DKHGLRAEFLELVKAAQRLSGDRVGAAIPPAWHVTARPVLATLPAPTLSASATGLKGE
                     HIFALGLAAGALISFATLVICIFLARSRTAAISFGPAPLARLK"
BASE COUNT          405 a          796 c          829 g          398 t
ORIGIN      
        1 tcagccaggc gtttgtggaa gggctcgaga cgttccgcca cctcaaagcg gctggcgacc
       61 tggctgagcg gctgcgtcag gcgagcctcg agctccggcg cgacgtggtg atggccaacg
      121 aaggcaccgc caccttcatc ggcgaaacgc tgctgaccgg cacgcccgac cgcgccgccg
      181 cagccgattt gcgggcccgc tatgaagcga tctgtgccgc ggctttggat ttggaatctc
      241 gtctccgcga ggtggaacaa cagacttcca acccggccgc ccgccgcttg ctcggccgga
      301 cgctggatgc gctccagggc gtaaagctaa gcgccgcgac gatgatcaag cttctcgacc
      361 tgatcgcgcc gccccaacgc gactaagcct gagggcttaa ggcctggcat gctgctggaa
      421 gaacttgacg aacagggccg gggcttcagg cggaaagttg tgagcgcccg ggtggatgaa
      481 ggtgacgacg ggcgtgccgg acttcgactc atacagggtg cagcctttgg cccactcgga
      541 gccggtcggt tcgcagccgt tgagcttgcg gagcgcttcg atcgtcgcct gctgccactc
      601 gtacttgacg agttggtcat tctcgccggc caggtgcatc accggcttgg gcttgaggtc
      661 ggcgaggcgg ctggtccacg cagcgccggg gccggcagcc gcggagggcg ccatcgcggc
      721 aaagacgtcg ccgcgcgtcc gccagagcag gtaggtgaag ccgccgccgt tggagtggcc
      781 ggtggaatag atgcggcgct ggtccacctt gtagtcggcc tggagcgacg cgaggacggc
      841 atcgaagaac ttgagatcgc gatcgcccag tatcccggcc gcattttgcc agccgggccg
      901 cttgccctcc ggatcggtca gccgaccggg cgtgttcagg ccctgcatgt aaacgacgat
      961 cgcctcgggc cagacgtcgt ggtagtggaa catcacggcc gcccgctgca tcgtgccgcc
     1021 gtggccgtga aaggcgaaga cgaccggcga cggcgtggtc ttggcggctg cgggaacgta
     1081 gagcagcgcc tcgcgcggcg tatcgccgat ggtccactcg cggcgggtca gctcctgctt
     1141 ctcgggcgtt gcttgagcgc gggccagcgc ggcaaggagt gccagcagca acgcaccacg
     1201 cagaaccgcg acgaaaggtt tcacagtgca gctccgaagg ccaaacggga agctgcaccg
     1261 cagtatcgca gcggcaggcc acggctggca agctacttga gcctggccag cggcgcggga
     1321 ccgaaggaga tggcggctgt gcgggaccga gcaaggaaga tgcagatcac caaggtggca
     1381 aaggagatca aggctccggc ggcgaggccg agcgcgaaga tgtgctcgcc tttcaggccc
     1441 gtagccgacg ctgacagcgt cggcgctggc agcgtcgcca acactggacg cgcggtgaca
     1501 tgccacgcgg gcggaatcgc cgcgccgact ctgtcgcccg aaagccgctg ggccgctttg
     1561 accagctcca gaaactccgc ccgcaggccg tgcttgtcct ggcccttcgc ggccgacgcc
     1621 gtttccagca ccgcggcgaa cgaaacgtct cccttgtact ttgagttgcg aagcaattgc
     1681 ccgaacgacg cgaccgccgc ggcaaactga aaatcgggcg acgccgagcc aaaccgctgg
     1741 ccgctgtcct tcaccggaaa cgtcagcagc gtgctggtcc cgccagccgg cggctggtag
     1801 cgcagcttca gggtgagcat ttcgcccgac ttcgcggcct cggtgagcgg aacgccatcg
     1861 ggaggctcac gcctcccgct cgccgctgtc tcctggtact tcagcggatc gacgcccggc
     1921 cgccccagct cgctctcaac gccgacaggc acgacctgat acagggccgt gaccgagtgc
     1981 cccgcgccga tgtcgccggc gtccttcgtg tcgtcgttga agtcgcggtc ggccaggcgg
     2041 cggttctcgt agccgatcag ccgataggcg gcgacctcgg ccggattgaa ctcgacctgg
     2101 atcttcacgt ccttggcgat cgtgaccagc gtgccctgca tctgctcgac gaagaccttc
     2161 tgggcttcgc ggtcgctgtc gatgaaggcg tagttgccgt cggccttgtt ggcgatctgc
     2221 tcgagcatcg cgtcgttgtg gttgccgatg ccgaagccga gcacgctcag gtggacgccg
     2281 ccccgcgatt tctcgatcgc cagccgcacc aggtcgcccg tcgaggtcgt gccgacgttg
     2341 aagtcgccgt cggagcagag gatcacgcgg ttgacgccgc cccggatgag attctcctgg
     2401 gcgatcttgt acgccaggtg aatgccct
//
LOCUS       DASRJB010000151         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2299019, whole genome shotgun sequence.
ACCESSION   DASRJB010000151 DASRJB010000000
VERSION     DASRJB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299019"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFB80_04310"
     CDS             <1..685
                     /locus_tag="VFB80_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN33013.1"
                     /translation="FAHHRRRCPAIANGPADVRNNVVYNFRDGFHHDNPPKGGGYNVI
                     GNYYKRGPSDPKIFPFCFQGTTEYFLRDNYIDGVGAVQDPWAESGKLAGLAYYAAKGK
                     KAERETAVPKVTTHSPQEAYELVLQQAGGLPRDAVTARIVAEVKNGTGSWGKKPQDDL
                     LAGLTPGKPLADSDSDGLPDDWEATHGLDPKNGSDHSRAMPSGYPAIEEYANELAAAL
                     IKAAQTERP"
     gene            complement(689..1987)
                     /locus_tag="VFB80_04315"
     CDS             complement(689..1987)
                     /locus_tag="VFB80_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN33014.1"
                     /translation="MTVVALALAGCGPAAVAQPQAAPAAGGALEVVQAGNPTRKTLTL
                     TTAQPARIEALEQTPIHSKLAAYVGEVLVDYGDRVKPGQPLVKLTAPELDAELAQKRA
                     LLEQARAEIAQAEAARAATEAAVLASRALVDQAEAATLRSQADVARWKSEYARIEQLA
                     TSGAVNRQLVDETQQKYRAAEADQKEALSAVESAKAALVQSQSQVTKAAADVTAAQAR
                     QRVAEANVAAALAQLSYLSLNAPFAGVVTHRRVDPGHFVQPGANNSPPLLVVARADKL
                     RAFIAVPEAEAAYVDVGDAVTLDVQSLRGAEFPGQVSRTGFALSEGSRSLETIVDLDN
                     AAGKLRPGLYATARIVLQEQKDALVLPSAAVVRQNKEAFCYRLIGGQATKTPIQLGIR
                     VADEWEIAAGLTERDLVILNKAAALKDGQPVERLKAEAKK"
     gene            complement(2121..>4523)
                     /locus_tag="VFB80_04320"
     CDS             complement(2121..>4523)
                     /locus_tag="VFB80_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN33015.1"
                     /translation="YALVNGRRAVYILATKRADASTLSVIHAIKRALPEMQKKLPGDI
                     HVSFEFDQSPYVTRAVAGVVTEGTLGAVLVGLMVLVFLWDWRSALVVVLNIPLALMSA
                     VCALWICGYTINVMTLGGLALAIGVLVDEATVEIENIHAQMSHTPSIARAVQLGNAVT
                     ALPRLLAMLCVLAVFIPAFFMEGAARALFAPLALAVGFSMIASYVLSSTFVPVMSVWL
                     LRYSKPRPVVAVSAVPATTGRGFDRLRRGYERLLGGLVAFRWILVPAYLAACAAIVLA
                     VLPRLGREIFPLVDTGAFRLRLRAPDGTHIGKSEETAKKALDIVAQTVGPGNVALSLG
                     YVGMIPSTFPVNAVYQWSRGPEEAILYVAFKDGARIDVERLKETLREKFTAELPGVRA
                     SFEPADIINDVMSFGSPTPIEISVSGGNFAESRRHAEKIRDELAKLPYLRDLQFGQSL
                     DYPTIEVNVDREKAGLAGLTASDVSRSLVTATSSSRFTVPNYWADPKTGIAYQVQVEI
                     PRPVVRSPDGIDPTNSPEEIGQIPLAKNGDGLVLIRDVAEIRQGTMVGQYDRYNMKRQ
                     VTLTANRSGVDLGTLTREVAAAVKRAGEAPQDAKIEIRGQIPPLNEMSSGLAAGLIVS
                     VITVFLLLTANFQSVRLAVVAVSSVPAVVAGVVVTLWLTGTTLNIQSFIGAIMAIGVA
                     MANAILLVTFAESRRREGERSLAAAVSGAGSRLRAILMTSCAMIAGMTPMALAIGEAG
                     AQNAPLGRAVIGGLAAATLATLLVLPAIFALLARRDDFQTASLDPDDPESRHYLIPAD
                     GA"
BASE COUNT          760 a         1543 c         1541 g          679 t
ORIGIN      
        1 gttcgcccac caccggcggc gctgcccggc gatcgccaac ggcccggccg acgtccgcaa
       61 caacgtggtg tacaacttcc gcgacggttt ccaccacgac aatccgccga agggcggcgg
      121 ttacaacgtc atcggcaact actacaagcg cgggcccagc gaccccaaga tctttccgtt
      181 ctgctttcag ggcacgaccg agtacttcct gcgggacaac tacatcgacg gcgtcggcgc
      241 cgtgcaggac ccgtgggccg agagcggcaa gctcgccggc ctggcctact atgccgccaa
      301 ggggaagaag gccgagcggg agacggccgt gccgaaagtg acgacccact cgccgcaaga
      361 ggcttatgag ctggtgctcc agcaagccgg cggcctgccg cgcgacgccg tcacggcgcg
      421 gatcgtcgcc gaggtcaaga acggcaccgg ctcctggggc aaaaagccgc aggacgattt
      481 gctggctggc ctgacgcccg gcaagccgct ggccgattcg gacagtgacg gcctgcccga
      541 cgactgggag gccacccacg ggctcgatcc caaaaacggg tccgatcact cacgggcaat
      601 gcccagcggc tatccggcga tcgaggagta cgccaacgaa ctggcggccg cgctgatcaa
      661 ggcggcccag accgagcggc cctagcagct atttcttcgc ttcggccttc agccgctcga
      721 cgggctggcc gtccttgagg gcagcggctt tattgaggat cacgaggtcg cgctccgtca
      781 ggccggcggc gatctcccac tcgtcggcga cgcggatgcc gagctgaatc ggcgttttcg
      841 tcgcctggcc gccgatcagg cggtagcaga acgcctcctt gttctgccgg acgacggcgg
      901 cggagggcag caccagcgcg tccttctgct cctgcaggac gatccgcgcc gtggcgtaga
      961 gccccggccg gagcttgccg gcggcgttgt cgagatcaac gatcgtttcc agcgaacggc
     1021 tgccttcgct gagggcgaaa ccggtgcgcg agacctgccc cgggaattcc gcgccgcgga
     1081 gcgactgcac gtcgagcgtg acggcgtcgc cgacatcgac ataggccgct tcggcctccg
     1141 gcacggcaat gaacgcccgc agcttgtcgg cccgggcgac gaccaggagc ggcggcgaat
     1201 tgttggcgcc gggctgcaca aaatgccccg gatcgacgcg gcggtgcgtg acaacgccgg
     1261 caaacggcgc attaagcgac aggtacgaca gctgggccag ggccgcggcg acattggcct
     1321 cggccacgcg ctggcgggcc tgcgcggcgg tcacgtcggc cgcggccttg gtgacttgcg
     1381 actgcgactg aacgagcgcc gccttggcgg attcgaccgc cgacagggct tccttctggt
     1441 cggcctcggc cgcgcggtat ttctgctgcg tctcatcgac gagctgccgg ttgaccgcgc
     1501 cgctagttgc gagctgctcg atgcgggcgt attccgattt ccagcgggcg acgtcggcct
     1561 gcgagcggag cgtggccgct tcggcctggt ccaccagcgc ccggctggcc agcacggcag
     1621 cttcggtggc cgcgcgggcc gcctcggcct gcgcgatctc cgcgcgagcc tgctccagca
     1681 gggcccgttt ctgggcgagc tcggcgtcga gctccggcgc ggtgagcttc accagcggct
     1741 gccccggctt cacacggtcg ccataatcga ccagcacctc gcccacgtag gccgccagct
     1801 tcgagtggat cggcgtctgc tccagcgcct cgatccgcgc cggctgagcc gtcgtgagcg
     1861 tcagcgtttt gcgggtcgga ttgccggcct gaacgacctc cagcgcgccg cccgccgcgg
     1921 gagcggcttg cggctgggcg acagcagccg gcccgcagcc ggcgagggcc agcgcgacga
     1981 ccgtcatcgc gcgctgtagg gaacgccctc tgtggcgttc cggcgtcgcg agtggacgat
     2041 cgatcccagg gcacggaatg ccacggaggg cattccctac agaacccacg ctcgagctag
     2101 gggcgggtct ttgtctcatc tcaggctccg tccgcgggga ttagataatg gcggctctcc
     2161 gggtcgtccg gatcgagcga cgccgtctgg aagtcgtccc gccgggcgag cagcgcgaag
     2221 atcgcgggca gaaccagcag cgtggccagc gtggccgcgg cgagcccgcc aatgacggcg
     2281 cggcccaggg gggcgttttg tgcgccggcc tcgccgatgg ccagcgccat cggcgtcatg
     2341 ccggcgatca tggcgcagct ggtcatcagg atcgcccgca gccgcgagcc cgcgccggac
     2401 acggccgctg ccagcgagcg ctcgccttcg cggcggcggc tctcggcaaa cgtgaccaac
     2461 agaatcgcat tggccatcgc cacgccgatc gccatgatcg cgccgatgaa cgactggatg
     2521 ttcagcgtcg tgccggtcag ccagagcgtc accacgacgc ccgccacgac cgccggcacg
     2581 gacgaaacgg cgaccacggc cagccgcacg gactgaaagt tggccgtcag cagcagaaac
     2641 accgtgatga ccgagacgat caggccggcg gccaggccac tgctcatttc gttcaggggc
     2701 ggaatctggc cgcggatttc gatcttcgcg tcctggggcg cttcgccggc ccgcttgacc
     2761 gccgcggcta cctcgcgggt gagcgtgccc agatcgacgc ccgagcggtt cgcggtgagg
     2821 gtcacttgcc gcttcatgtt gtagcggtcg tactggccca ccatcgtgcc ctggcggatc
     2881 tcggcgacgt cgcggatcag caccagcccg tcgccgttct tcgccagcgg aatctggccg
     2941 atctcttcgg gcgagtttgt agggtcgatg ccgtccggcg agcggacgac cggccgcggg
     3001 atctcgacct gcacctggta ggcaatgccc gtcttcgggt cggcccagta attcggcacc
     3061 gtaaaccggc tgctggaggt cgccgtgacc agcgagcggc tgacgtcgga ggccgtcagg
     3121 ccggccaggc ccgccttctc gcgatcgacg ttgacctcga tcgtcggata gtcgagcgac
     3181 tggccgaact gcaaatctcg caggtagggc agctttgcca gctcgtcgcg aatcttctcg
     3241 gcgtgccgcc ggctttcggc aaaattgccg ccgctgacgg aaatctcgat cggcgtcggc
     3301 gagccgaagc tcatcacgtc gttgatgatg tcagctggct cgaagctggc tcgcacgccg
     3361 ggcagctcgg cggtgaactt ctcacggagt gtctctttga gccgctcgac gtcgatgcgg
     3421 gcgccgtcct tgaaggcgac gtaaaggatc gcctcctccg gtccgcgcga ccattgatag
     3481 acggcgttca ccggaaaagt gctggggatc atgccgacgt agcccaacga cagggcgacg
     3541 tttccggggc cgaccgtctg ggcgacaatg tcgagcgcct tcttggccgt ctcttcgctc
     3601 ttaccgatgt gcgtgccgtc gggcgctcgc agccgcaggc gaaaggcgcc ggtatcgacc
     3661 agcggaaaga tctcgcggcc cagccgcggc agcaccgcca gcacgatcgc ggcacaggcc
     3721 gccaggtaag ccggcacgag aatccagcgg aaagccacca ggccgccgag aagccgttcg
     3781 tagccgcggc ggaggcgatc aaagccgcga ccagtggtcg cgggaactgc agaaaccgcg
     3841 accactggtc gcggctttga gtaccgcagc agccagaccg acatcaccgg cacgaacgtg
     3901 ctcgacagca cgtacgacgc gatcatcgaa aaaccgacgg ccagggccag cggggcgaac
     3961 agcgcgcggg ccgcgccttc catgaagaac gccggaatga acacggccag gacgcagagc
     4021 atggccagaa gccgcgggag cgcggtcacg gcgttgccca gctgcacggc ccgggcgatc
     4081 gacggcgtgt gactcatttg cgcgtggatg ttctcgatct cgacggtcgc ttcatcgacg
     4141 agcacgccga tcgccagcgc caagccgccg agcgtcatca cgttgatcgt gtagccgcaa
     4201 atccagaggg cgcagaccgc cgacatcagg gccagcggga tattgagcac gacgaccagg
     4261 gcactgcgcc aatcccacag gaaaacgagc accatcaggc cgaccagcac cgcgccgaga
     4321 gtgccctcgg tcacgacgcc ggcgaccgcc cgcgtgacgt agggggactg gtcgaactcg
     4381 aagctgacgt ggatgtcgcc gggcagcttc ttctgcatct cgggcagagc ccgcttgatc
     4441 gcatgaatga ccgagagcgt cgaggcatca gcccgcttgg tcgccagtat atagacggcg
     4501 cggcggccgt tgaccagcgc gta
//
LOCUS       DASRJB010000152         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3426205, whole genome shotgun sequence.
ACCESSION   DASRJB010000152 DASRJB010000000
VERSION     DASRJB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426205"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1522)
                     /locus_tag="VFB80_04325"
     CDS             complement(74..1522)
                     /locus_tag="VFB80_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33016.1"
                     /translation="MTNFCGRTRREFLWQAGAGFTGVALAGMLEGDFFARQATAADGV
                     TKFANPLAPKKPHLPAKAKSVIFLYMYGGPSHMDTFEYKPAMVGMDNKTVNVKTFGRG
                     GHKNQGRIVEPRWKFKQYGQCGHWVSDLFPHLAQHVDDIAFLHSMTADSPIHGSAMLQ
                     MNTGKILSGSPCLGSWVNYGLGSENENLPGFVVMLDPRGGPISGAKNWSSGYMPAHYE
                     GTMFRSQGTPILDLDRPADMTEASQRRILDSLRSYNEEHAARQGGNQALTSRIASYEL
                     AFKMQATAPEATDLADEPQSIRDLYGLDDKQTSAVARQCILARRLVERGVRFVQIYSG
                     GAHNDDNWDAHGDLLANHNLHAGETDKPIAGLIADLRQRGLLDSTLIVWGGEFGRQPT
                     AEYEKGTGRDHNAYGFTMWLAGGGVKGGVSVGTTDELGSAAVEKPFHVRHLHATILHQ
                     LGIDPERLTYFYAGLDQKLVGVEGASPIREIL"
     gene            complement(1531..>2334)
                     /locus_tag="VFB80_04330"
     CDS             complement(1531..>2334)
                     /locus_tag="VFB80_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN33017.1"
                     /translation="FGYQGTPPTHPELLDWLASEFVHPTASGGRPASGGRKPTEWSLK
                     SLHRLILLSSAFQMSGQASPAAAKIDPENDLLSHFDLRRLTAEELRDSILAACGNLNV
                     KKEDGPSVYPEIPREVLAGQSMPGHNWGRSSPEEAASRSVFVHIKRSLALPILAAFDA
                     PDPDAPCPVRFTTTQPTQALALINSSFLAQQSRLLAKLAADKAGGETAAQVEFVLRRV
                     LQREPTAAEIDRGVKFIVQAQEAEKLAHEEALRQFCLVALNLNEFVYLE"
BASE COUNT          352 a          770 c          785 g          427 t
ORIGIN      
        1 ccgccaggag aacgccaagg gcggcggcca ccgttcgcat catcgcacgc tctccatgcg
       61 gggacgtggc gggtcagagg atttcgcgga tggggctggc gccctcgacg ccgacgagct
      121 tctggtccag ccccgcgtag aagtacgtca gccgctccgg gtcgatgccc agctggtgca
      181 ggatcgtcgc gtgcaggtgg cggacgtgga acggcttctc cacggcggcg gagcccagct
      241 cgtcggtcgt gccgacgctg acgccgccct tgaccccgcc gccggccagc cacatcgtga
      301 agccgtaggc gttgtggtcg cggccggtgc ccttttcgta ttcggccgtc ggctggcggc
      361 cgaattcgcc gccccacacg atcagcgtgc tatccaagag gccccgctgc cgcaaatcgg
      421 cgatcaggcc cgcgatcggc ttgtcggttt cgccggcgtg gaggttgtgg ttggccaaga
      481 ggtcgccgtg ggcgtcccag ttgtcgtcgt tgtgcgcgcc gccggaatag atctgcacaa
      541 aacgcacgcc gcgctcgacc agccgccgcg cgaggatgca ctgccgggcc acggcgctgg
      601 tctgcttgtc gtccagaccg tacaggtcgc ggatgctctg cggctcgtcg gccaggtcgg
      661 tcgcttccgg cgcggtggcc tgcatcttga acgccagctc gtagctggcg atccgcgacg
      721 taagcgcctg attgccgccc tgcctggcgg cgtgctcttc gttgtagctg cgaagcgagt
      781 ccaatatccg ccgctggctg gcctcagtca tgtccgccgg ccggtcgagg tcgaggatcg
      841 gcgtgccttg tgagcggaac atcgtgcctt cgtagtgggc cggcatgtag ccgctggacc
      901 agttcttggc gccgctgatc ggcccgccgc gggggtcgag catgacaacg aaccccggca
      961 gattctcgtt ctcgctgccc aggccgtagt tcacccacga gccgaggcac gggctgccgc
     1021 tcaggatttt gcccgtgttc atctgcagca tggccgagcc gtgaatcggc gagtcggcgg
     1081 tcatcgagtg gaggaacgcg atgtcgtcca cgtgctgggc caggtgggga aacagatcgc
     1141 tgacccagtg gccgcactgg ccgtattgct tgaacttcca ccgcggctcg acgatgcggc
     1201 cctggttctt gtgtccgccg cggccaaacg tcttcacgtt caccgtcttg ttgtccatgc
     1261 cgaccatcgc cggcttgtac tcaaaggtgt ccatgtgcga cgggccgccg tacatgtaca
     1321 ggaagatcac gctcttggcc ttagccggca ggtggggctt cttcggcgcc agcgggttgg
     1381 cgaacttcgt cacgccgtcg gcggcggtcg cctgccgcgc gaagaaatct ccctccagca
     1441 ttcccgccag cgccacgccg gtgaatccag cgcccgcctg ccacaagaat tcccgccgcg
     1501 tacggccgca gaagttggtc ataatgcgac ttactccaaa tacacaaact cgttcaggtt
     1561 caatgccacg aggcaaaact gccgcagggc ctcttcgtga gccagcttct cggcctcttg
     1621 ggcctggacg ataaacttca cgccgcggtc gatctcggct gccgtcggct cgcgctgcag
     1681 gacgcggcgc aggacgaact ccacttgcgc cgcggtttcg ccgccggcct tgtcagccgc
     1741 cagtttggcc agcagccgcg actgctgggc caggaagctg ctgttgatca gcgccagcgc
     1801 ctgtgtcggc tgcgtggtcg tgaaccgcac cgggcagggc gcgtcgggat cgggtgcgtc
     1861 aaacgccgcc aggatcggca gggccagcga ccgcttgatg tgtacgaaga cgctgcggct
     1921 ggcggcttct tccggcgacg agcggcccca gttgtgcccc ggcatcgact gcccggccag
     1981 cacctcgcgg ggaatttccg gatagacgct ggggccgtct tccttcttga cgttcagatt
     2041 gccgcaggcg gcgagaatcg aatcgcgcag ttcctcggcc gtcagtcgcc tcaggtcgaa
     2101 gtgtgagagc aagtcgttct ccggatcgat cttggcggca gccgggctgg cctggccgga
     2161 catctgaaag gcgctggaaa ggaggatcag gcggtggagg gacttcagcg accactcagt
     2221 gggcttacgc ccaccgctcg ccggcctgcc accgctcgca gtcggatgaa cgaactcgct
     2281 ggccagccag tcgagcagct ccgggtgcgt gggcggggtg ccttgatagc cgaa
//
LOCUS       DASRJB010000153         8574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3992116, whole genome shotgun sequence.
ACCESSION   DASRJB010000153 DASRJB010000000
VERSION     DASRJB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992116"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..906
                     /gene="rpsO"
                     /locus_tag="VFB80_04335"
     CDS             637..906
                     /gene="rpsO"
                     /locus_tag="VFB80_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZN33018.1"
                     /translation="MTVTKERKKELIQQYRRADSDSGSPEVQIAILTVRINNLTEHMR
                     THEKDYASRRGLLAMVSRRRTLLDYLRRVNPQGYVDIIGKLGIRK"
     gene            1079..3379
                     /gene="pnp"
                     /locus_tag="VFB80_04340"
     CDS             1079..3379
                     /gene="pnp"
                     /locus_tag="VFB80_04340"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZN33019.1"
                     /translation="MKVKVEKQIGSGVLSFESGLLAKQASAAVIARFGETVTLNTAVS
                     GPGRPGIDFFPLTCDYRERTAAAGKFPGGFLKRESRPTTKETLTSRLCDRPIRPLFPK
                     GFKEEVQCQSFVLASDKQNDGDVIAMNGVAAALFISPLPFQGPIASVRVGRIAGKFIP
                     FPTHDELEDSDLDLIVSGTADSVAMIEGFARELPENDMFEAILFAHATIREIIDLQRE
                     FARQLGVVKPEFVAPPDPGVYDRLKSRFYDEFKSAKQTSGKQNRAEAVKAIKERALKE
                     IIPDPLAADAVKPEVFSTAWHDLEERVVRDLILSGTRPDGRDYRSLRNIECLVDVLPR
                     VHGSALFQRGETQALITVTLGTTRDEQRVDGLFDEYSKKFMLDYNFPSFSVGEVRPIR
                     GPGRREIGHGALAERSVNPVLPASEEFPYTIRVISDILESNGSSSMASVCGATLGLMA
                     AGVPISNPVAGISVGLVKESDTQWVLLTDIIGDEDHFGDMDFKISGTQNGITGIQLDL
                     KIEGISPEIIRATFDQSREARIQILRKMLTAISRPKSDISEHAPRLIRTTIDPLKIGM
                     LIGPGGKNIRGIQEATGTVIEVEDDGTVTIASTNREWAEAALAQVEACTATVQIGKIY
                     NGRVTSIKDFGAFVEILPGRDGLVHVSELSTGYVSSVMDVCRIGDEMKVLVIDVDEHD
                     RVKLSRKRAHEELGIPDEMASQGVPAGGPPRDGEGGGDYRGGGDRGEYRGGGRGGDRG
                     GRGGRGGGRGGDRGGRGGGGGYRDRR"
     gene            3474..4232
                     /locus_tag="VFB80_04345"
     CDS             3474..4232
                     /locus_tag="VFB80_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN33020.1"
                     /translation="MSESTPLSTEQALDQLRERYAELAALAGSLAHEIKNPLSVIRMN
                     MDLLAEDFAQPQTPRERRALGKIDMVGRQCTRLETLLNDFLHFNKVGQLELRLGSLNE
                     QIDRVLDLFEPQARDTHSEIVRYLDPDLPSILLNGETLQAALVNLVKNALEAMPDGGM
                     LTVRTRVTRQGVALDLIDTGCGMDERTAMKMFDAFFTTKSGGSGLGLPTARRIIEAHG
                     GRISVHSELGRGTQFTLEFPTPPRLGDGQGQSEE"
     gene            complement(4234..4641)
                     /locus_tag="VFB80_04350"
     CDS             complement(4234..4641)
                     /locus_tag="VFB80_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN33021.1"
                     /translation="MLTSLDPALWPSPALLAFAKRQAAQQQLHLGAERRAEERLLYAM
                     PVVTYGVDEHIRPVADPQAMVVRDHSAKGLGLVYERPFSHRKIVVELTYPEEGKFLAA
                     EVLWSKPLGPFYLLGCEIIAKLDAFPAARTLTA"
     gene            4891..6327
                     /locus_tag="VFB80_04355"
     CDS             4891..6327
                     /locus_tag="VFB80_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN33022.1"
                     /translation="MADERTTEAAEPAAQALRLLVVDNDEALARAMEESLVKVGYECV
                     VATSGPEGARKIETDTFDIVVTDLVMNDVDGMEILGRSRQRLPDAEVILVTGHATVPK
                     AVEAMQQGAFNFLEKPITPAKLRAVCEKAADAIRLKRQNTELRERLDERFGFEGIIYA
                     SSQMQTVIDRLKRIAPTDATVLIAGESGTGKELIAQAIHQNSPRKGKRIVALNCAAVA
                     ENLVESELFGHVKGAFTDAHSDRIGAFEFANGGTLFLDEVGDMPISTQIKLLRVLEEH
                     QITRVGDNKSIRVNVRVVSATNRPLEDLVAAGKFRNDLYFRLKVVTVPLPALRERRDD
                     IIPLADHFRRQFAKRHHKSIKGITPAATKRLFGFDWPGNVRQLRNALETMVVLDLDGT
                     LDIDDLPPELADAPPAAATSAAGGPTELIGRSMEEIERWAIEETLKLTGGNREEAAKI
                     LAIGARTLYRKLEKYQTGAASTDEDDGQ"
     gene            6412..8352
                     /gene="mutL"
                     /locus_tag="VFB80_04360"
     CDS             6412..8352
                     /gene="mutL"
                     /locus_tag="VFB80_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZN33023.1"
                     /translation="MPQIRQLPPSVVNKIAAGEVIERPASVVKELLENAVDAGARRID
                     VALGQGGTDLVRVVDDGCGIAAEDLPLAIASHATSKIADADDLFRVATFGFRGEALAS
                     IAEVSHLTLRSRPAAEGSGHEIEVGGGTRREIAPCGCPIGTTIEVRNLFYNTPVRRKF
                     LRASQTEIGHCTEAFTRLALAQPQIHFTLRHNDKTLFDLPPTEVWRERIVALVGRELE
                     PALIPLEGRDGDVRLTGFVADPEHSRANNRLQYMLLNGRFIRDRALQHALGEAYRGLL
                     LGGRFPIAFLRLEVPATAVDVNVHPTKLEVRFADSGRIYALLLGTIRKKFLATDLTAH
                     VRAPQGTEQPGAEAADAAAAQEHRRSLVAWATGALPASALPASAPATGAATALAEGDE
                     SQALLELRFAPPAGPPLEIHRLDRAWPAAPLSRERHEAAEYAGSRTAQAPPPAPPRLD
                     PPEPPRRSHLGFQIHNRYLVTENDEGMVVVDQHALHERILYEQLREKVLAGGMETQRL
                     LVPEPVTLPPADAAAALAAAETLGKLGIEVEPFGGDTVLVASYPAMLANLSPAEMLRQ
                     VVELLVTSPKTPERRDLLDDLLHMIACKAAIKAGDRLSPEEVTALLEQRHHFQDSHHC
                     PHGRPTALVFTREELDRRFKRT"
     gene            complement(8363..>8574)
                     /locus_tag="VFB80_04365"
     CDS             complement(8363..>8574)
                     /locus_tag="VFB80_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZN33024.1"
                     /translation="GVDPLAPALAATFGSGLGFMLPVSTPCNAIVYSSGLVPLRKMMA
                     YGLVLDIVGCAVIIAAVQLLVPLTR"
BASE COUNT         1608 a         2827 c         2858 g         1281 t
ORIGIN      
        1 tctgcgttac gcataatatg caacttaagc ggtgtcgtcg caagtcattg cgcacacagt
       61 agttgcgatg atgcatgtgc gggaaaaaat gcggcggcat gcatatgcag cataaggggt
      121 gagctgtggc tgaggctgag aactcgcagc ggtaaaccgc gaccactggt cgcggctttg
      181 gagcgcgcat aatgcgcata attggtatcg ggcgtttttc gtcgtaagac attgcagcaa
      241 ttggagttgc gtcaccgatt gccgtttgga gaattatctc cgataagcgc gcatacccgt
      301 cgccgatacg tgcgtcccga gcctgcactc cctcgcctgc gcgccctgtt ggcattcaaa
      361 cacggtggtc ggaagtcgtg gcggcggcga ggcttgcggc gagtcgcgta cagctcacag
      421 cggcagtttg gctgcccttg caatccccca gcagactgat acattgtgtg gcttgtcgta
      481 gtttttggct tcgtgtcaaa gaaaacagtt ggaagttcag gtaaccacag tcccgcaaga
      541 gcagcagtag tcgaaggaga ttcgtcgcac ccaggcggcc gtccgcgaca aaaacaagtg
      601 gggcggacgc accgcgggca gtctcggcga gaagcaatga cagtcaccaa agagcggaaa
      661 aaggaattga tccagcagta tcggcgggcg gactctgact ccgggtcgcc ggaagtgcag
      721 attgcgattc ttacggtccg gattaacaac ctgaccgagc acatgcggac gcacgaaaag
      781 gattatgcca gccggcgagg ccttttggcc atggtcagcc gccgccgcac tttgctggac
      841 tatctgcgcc gcgtcaatcc acaggggtat gtggacatca ttggcaagct cggtatccgc
      901 aagtaggcct gtcccggcgg caaacggttg ccgccggccg gcgcaagagt gccggaaatt
      961 cttcttggcg agtttacgcc cggccgcatg cgcgtgactg gcacgcgcgg cagctttcgt
     1021 gcacgctcca ccggcaactg acgttcgcag caggacccag cgtaaaggca ggacaatagt
     1081 gaaggtcaaa gtcgaaaagc aaattggcag cggcgttctc tcgttcgaaa gcggcctctt
     1141 ggccaagcag gccagcgccg cggtgatcgc ccgcttcggc gaaaccgtca cgctcaacac
     1201 ggcggtcagc ggccccggcc gcccggggat cgatttcttt ccgctcacct gcgactatcg
     1261 cgaacggacc gcggccgccg gcaaattccc cggcggcttc ttgaagcggg agagccgccc
     1321 aaccaccaag gagacgctca cttcgcggct ctgcgaccgg cccatccgcc cactgtttcc
     1381 caagggattt aaggaggagg tgcagtgcca gagcttcgtg ctggccagcg acaagcagaa
     1441 cgacggcgac gtgatcgcca tgaacggcgt ggccgcagcc ctgttcatct cgccgctgcc
     1501 gttccaggga ccgattgcca gcgtccgcgt cggccgcatt gccggcaagt tcatcccgtt
     1561 ccccacgcac gacgagctgg aagacagcga cctggacctg atcgtctcgg gcacggccga
     1621 ctcggtggcg atgatcgagg gcttcgcccg cgagctgccc gaaaacgaca tgttcgaggc
     1681 gatcctgttc gcccacgcca caatccgcga gatcatcgac ctgcagcgcg agttcgcccg
     1741 ccagctcggc gtcgtcaagc ccgagttcgt ggccccgccc gatcccggcg tttacgaccg
     1801 gctcaagagc cgcttctatg acgagttcaa atcggccaag cagaccagcg gcaagcagaa
     1861 ccgggccgag gcggtcaaag caatcaaaga acgggccctg aaggagatca ttcccgatcc
     1921 gctggcggcg gacgccgtca agccggaggt gttctcgacc gcctggcacg acctggaaga
     1981 gcgggtcgtc cgcgacctga tcctcagcgg cacgcggccc gacggccgcg actatcggag
     2041 cctgcggaac atcgaatgcc tggtcgatgt gctgccgcgg gttcacggct cggccctgtt
     2101 ccagcggggc gagacgcagg cgcttattac ggtcacgctg ggcacgaccc gcgacgagca
     2161 gcgggtggac ggcctgttcg acgagtacag caagaagttc atgctcgact acaacttccc
     2221 gtcgttctcg gtcggtgaag tgcgaccgat ccgcgggccg ggccgccgcg aaatcggcca
     2281 cggggcgctg gccgagcgaa gcgtcaaccc ggtgctgccg gccagcgaag agtttcccta
     2341 cacgatccgc gtgatctcgg acatcctcga gagcaacggc agctcgagca tggccagcgt
     2401 ctgcggcgcg actctcggcc tgatggcggc gggcgtgccg atcagcaatc ccgtggccgg
     2461 gatctcggtt ggcctggtca aggagtccga cacgcagtgg gtcctgctca ccgatatcat
     2521 cggcgacgag gaccatttcg gcgacatgga tttcaagatc tccggcacgc aaaacggcat
     2581 caccggcatc cagctcgacc tgaagatcga aggcatcagc cccgagatta tccgggccac
     2641 gttcgaccag tcgcgcgagg ctcgcatcca gattctccgc aagatgctga cggcgatttc
     2701 gcggcccaag agcgacatct cggagcacgc tccgcgcctg atccgcacga cgatcgaccc
     2761 gctcaagatc ggcatgctga tcggccccgg cggcaagaac atccgcggca tccaggaggc
     2821 gactggcacc gtcatcgagg tcgaggacga cggcaccgtc acgatcgcca gcaccaaccg
     2881 cgagtgggcc gaggccgccc tggcccaggt cgaggcctgc accgccaccg tgcagatcgg
     2941 caagatctac aacggccgcg tcacgagcat caaggatttc ggcgcgttcg tcgaaatcct
     3001 gcccggccgc gacggcctgg tgcacgtcag cgagctctcg acgggctacg tcagctcggt
     3061 gatggacgtc tgccggatcg gcgacgagat gaaggtgctc gtgatcgacg ttgacgagca
     3121 cgaccgcgtg aagctcagcc gcaagcgggc ccacgaagag ctggggatcc ccgacgagat
     3181 ggcctcgcag ggcgtgccgg ccggcggccc gccgcgggac ggcgagggcg gcggcgacta
     3241 tcgcggcggt ggcgaccgcg gcgagtatcg cggcggaggc cgcggcggcg accggggcgg
     3301 acgcggcggt cgcggcggcg gacgtggggg cgaccgcggt ggccgcggcg gtggcggtgg
     3361 ttatcgcgac cggcggtagc ctcttttgcc aggaccaaac tggtcccgga tcgcctggat
     3421 agacgcttga tccgcagttc gccgggacca ggttggtccc ggctgagaac agcatgtctg
     3481 aatccacgcc gttatcgacc gagcaggctc tcgaccagct ccgcgagcgc tatgccgagc
     3541 tggcggcgct ggccggctcg ctggcccacg agatcaagaa cccgctctcg gtcatccgca
     3601 tgaacatgga tctgctggcc gaggacttcg cccagccgca gacgccgcgc gagcggcggg
     3661 ccctgggcaa gatcgacatg gtcggccggc agtgcacgcg gctggagacg ctgctcaacg
     3721 actttttaca cttcaacaag gtcggccagc tcgaattgcg gctgggcagc ctgaacgagc
     3781 agatcgatcg ggtgctggac ctgttcgagc cgcaggcccg ggacacgcac agcgaaatcg
     3841 tccgctacct ggacccggac ttgccgagca tcctgctcaa cggcgagacg ctgcaggcgg
     3901 cgctggttaa cctggtgaag aacgccctgg aggcgatgcc cgatggcggc atgctgaccg
     3961 tgcggacgcg cgtcacgcgt caaggcgtgg ccctggacct gatcgacacg ggctgcggca
     4021 tggatgagcg gacggcgatg aaaatgttcg acgcgttctt cacgacgaag agcggcggct
     4081 cggggctggg cctgcccacc gcccggcgga tcatcgaggc ccatggcggc cggatcagcg
     4141 tgcacagcga gctcggccgc ggcacgcagt tcaccctcga attccccacg ccgccgcgcc
     4201 tcggcgacgg tcagggacag agcgaggagt gaatcaggcg gtcagcgtgc gggctgctgg
     4261 aaacgcatcg agcttggcga tgatctcgca gcccaggagg tagaaggggc cgagcggttt
     4321 gctccacagc acttcggcgg cgaggaactt gccctcttcg ggataggtca gctcgacgac
     4381 gattttgcgg tgcgaaaagg gccgctcgta gaccaggccc agccctttcg cggaatgatc
     4441 gcggacgacc atcgcctgcg ggtcggcgac gggccggatg tgttcgtcca cgccgtaagt
     4501 gacgacgggc atggcgtaga gcaggcgctc ctcggcacgg cgctcggcgc ccaagtgcag
     4561 ctgctgctgc gcggcctggc gcttggcgaa cgccaaaagc gcgggggacg gccacaaggc
     4621 cggatcaagt gaagtcagca tgggtccgct tctgatcgag tgaaccgctc atgcgcggca
     4681 gaaaccatac aaacggccca gccgcggcaa tgcggccggc cgcaatcgtt tcgacgagaa
     4741 gtgggatggg tagcggcgcc gctgaaaacg atgccgccaa aagcaaccgt aggccgaaac
     4801 cgtaccgtag tcaaatcgcc actttgtcca cgttttgctg gcgattctcg cccctcgcgg
     4861 ctggaaaccg cagtcgctat catcgccctg atggccgacg aacgaaccac ggaagcggcg
     4921 gagcccgcgg cgcaggccct gcggctgctc gtcgtggaca acgacgaagc cctggcccgg
     4981 gcgatggagg agagcctggt caaggttggc tacgaatgcg tcgtggccac cagcgggccg
     5041 gaaggggcgc ggaagatcga gaccgacacg ttcgacatcg tcgtcaccga cctggtgatg
     5101 aacgacgtgg acgggatgga gatcctcggc cgcagccggc agcgactgcc cgacgccgag
     5161 gtgatcctgg tcaccggcca tgcgacggtg cccaaggccg tcgaggcgat gcagcagggg
     5221 gcgttcaact ttttggagaa gccgatcacg cccgcgaagc tgcgggccgt ctgcgaaaag
     5281 gcggcggacg cgatccgcct caagcggcag aacaccgagc tccgcgaacg gctcgacgaa
     5341 cggttcgggt tcgagggaat catctacgcc agcagccaga tgcagacggt catcgaccgg
     5401 ctgaagcgga tcgccccgac cgacgcgacg gtgctgatcg ccggcgaaag cggcaccggc
     5461 aaagagctga tcgcccaggc gattcaccaa aacagcccgc ggaagggcaa gcggatcgtc
     5521 gccctcaact gcgcggccgt cgccgagaac ctggtggaga gcgagctgtt cggccacgtg
     5581 aagggcgctt tcaccgacgc ccacagcgac cggatcggcg cgttcgagtt cgccaacggc
     5641 ggcacgctgt tcctcgacga ggtcggcgac atgccgatca gcacgcagat caagctgctc
     5701 cgcgtgctcg aggaacacca gatcacccgg gtcggcgaca ataagtcgat ccgcgtcaac
     5761 gtccgcgtgg tctccgccac gaaccggccg ctggaagatc tggtcgccgc cggcaagttc
     5821 cgcaacgacc tctatttccg cctgaaggtc gtcaccgtcc cgctgccggc ccttcgcgag
     5881 cggcgcgacg acatcatccc gctggccgac catttccgcc gccagttcgc caagcggcac
     5941 cacaagtcca tcaagggaat cacgcccgcc gcgaccaaac ggctgttcgg cttcgactgg
     6001 cccggcaacg tgcggcagct ccgcaacgcg ctggagacga tggtcgtcct cgacctcgac
     6061 ggcacgctcg acattgacga cctgccgccg gaattggccg atgcgccccc ggccgcggca
     6121 acctcagcgg ccggcggccc gaccgagctg atcggccgct cgatggagga gatcgagcgg
     6181 tgggccatcg aagaaactct caagctcacc ggcggcaacc gcgaggaggc cgcgaaaatc
     6241 ctggccatcg gcgcgcggac gctgtatcgg aagctggaga agtaccagac gggggctgcc
     6301 tcgaccgacg aggacgacgg acaatagtca gttgtcattg gtcacctgtc atttgtcatt
     6361 tggggagagc cagcaaatga caaatgacaa gtgacatcca cggagggttc gatgccgcag
     6421 attcgtcaat tgccgccgag cgtcgtcaac aagatcgccg cgggcgaagt gatcgagcgg
     6481 cccgcgagcg tggtgaagga gctgctggag aacgcggtcg atgccggggc gcggcggatc
     6541 gatgtcgcgc tgggacaggg aggcacggac ctcgtgcggg tcgttgatga cgggtgcggg
     6601 atcgcggccg aggacctgcc gctggcgatc gccagccatg ccacgagcaa gattgccgac
     6661 gccgacgact tattccgcgt cgccacgttt ggttttcgcg gcgaagcgct ggcctcgatc
     6721 gccgaagtca gccacctcac gctccgcagc cggcccgcgg ccgaaggcag cggccacgag
     6781 atcgaagtcg gcgggggaac gcggcgcgag atcgctccct gcggttgccc gatcggcacc
     6841 acgatcgaag tccgcaatct gttttacaac acgccggtgc ggcgcaagtt cctgcgggcc
     6901 tcgcagaccg agatcggcca ctgcaccgag gccttcacgc gcctcgccct ggcccagccg
     6961 cagattcact tcacgctgcg gcacaacgac aagaccctgt tcgacttgcc gccaaccgaa
     7021 gtctggcggg agcggatcgt ggccctggtc ggccgcgagc tcgagcccgc cctgatcccg
     7081 ctggagggcc gcgacggcga cgtgcggctg acgggattcg tcgccgaccc cgagcacagc
     7141 cgggccaaca accgtctgca atacatgctg ctcaacgggc ggttcatccg cgaccgggcc
     7201 ctgcagcacg cgctcggcga ggcctaccgc ggcctcctgc tgggcgggcg ctttccgatc
     7261 gccttcctgc ggctggaagt gccggccacc gccgtggacg tcaacgtgca tccgaccaag
     7321 ctggaggtgc ggttcgctga cagcgggcgg atttacgcgc tgctgttggg caccatccgc
     7381 aagaagttcc tggcgaccga cttgacggcg cacgtccgcg cgccgcaggg aaccgagcag
     7441 cccggcgctg aagccgccga tgccgccgcc gcccaggagc atcgccgcag cctcgtcgcc
     7501 tgggccaccg gcgcactgcc cgccagcgca ctgcccgcca gcgcgccggc caccggcgcg
     7561 gcgaccgccc tggccgaggg tgacgagtcg caagcgctgc ttgagctgcg attcgcgccg
     7621 ccggccgggc cgccgctgga aatccaccgg ctcgaccgcg cctggcccgc cgcgcccctc
     7681 tcgcgagagc ggcacgaggc ggccgaatac gccggcagcc gcacggctca agcaccgccg
     7741 ccggcgccgc ctcggctcga tccaccagag ccgccacggc gctcgcacct gggttttcag
     7801 atccacaacc gctacctggt cactgagaac gacgagggaa tggtcgtcgt cgatcagcac
     7861 gccctgcatg agaggattct ctacgagcag ctccgcgaga aagtcctcgc gggcggcatg
     7921 gagacccagc ggctgctcgt ccccgagccg gtgacgctgc cgccggccga cgcggccgcg
     7981 gcgctggccg ccgccgaaac gctgggcaaa ctggggatcg aggtcgagcc cttcggcggc
     8041 gacacggtgc tcgttgccag ctatccggcg atgctcgcca atctcagccc cgccgagatg
     8101 ctgcggcagg tcgtcgaatt gctggttacc agccccaaga cgccggagcg ccgcgacctc
     8161 ttggacgacc tgctccatat gatcgcctgc aaagcggcga tcaaggccgg cgaccggctc
     8221 tcgcccgagg aggtcacggc actgctggag cagcggcacc acttccagga ctcgcaccac
     8281 tgcccgcacg gccggccgac ggccctggtc ttcacccgcg aagaactgga tcggcggttc
     8341 aagcgaacct gagagctgcg gctcaccgcg tcagcgggac cagcagttgt accgcggcga
     8401 taatcaccgc gcagccgaca atatcaagca cgaggccata ggccatcatc ttccgcagcg
     8461 gcaccaggcc cgagctgtag acgatggcgt tgcagggcgt cgagaccggc agcatgaagc
     8521 ccaggccgga gccgaacgtc gcggccaagg ccggcgcgag cggatcgacg ccgg
//
LOCUS       DASRJB010000154         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2015146, whole genome shotgun sequence.
ACCESSION   DASRJB010000154 DASRJB010000000
VERSION     DASRJB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015146"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="VFB80_04370"
     CDS             complement(<1..2264)
                     /locus_tag="VFB80_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN33025.1"
                     /translation="MRSLGAISGWLVAALLASLLAAPLAAEDAEAELAQRFAETIGPL
                     VKTHCHGCHGETKQEGKLKLSRFTSAAAVGKDHRVWEMVRQRLAAGEMPPEEAARKLA
                     DGDRKTLLAWIDAFREFEAERHAGDPGEVLARRLNSAEFDYTIRDLTGVDIRPTKEFP
                     VDPANEAGFDNSGESLAMSPALLQKYLAAARQVADHAVLLPEGFRFAPHAAVTETDRD
                     KYCVHRIIEFYKRHEVDLADYFLAAWRYRHRAALGRPEQRLADVAADARLSGKYLERI
                     WRLLTDAELPAAGPVAAIRTQWQELPAPASRDAAAPQEECESLRVEALWLRKELATEP
                     PKLSVKGISDGTQPFVLWRNRQGAERHTKYLGNAAADAERLRQKYVGTGHKLAAVFAV
                     DPSDAAAVKQFAAACEQFCEGIPDAFVVSDRGPYFDPKQAGKGRFLTAGFHLMQGYYR
                     DDQPLADLILTDAERRELDGLWQELNFITQVPLRQYQDFIFFERAEPPRFMYDAQFDF
                     ARSEDKDAASPEKMRRLQELHLALAKKKGAGPDALAAIETYYANIEAEIRWVERARLA
                     AEPSHLQALADFAERAYRRPLTAAEREELLAFYRRLREKDRLPHEEAFRDTLASVLLS
                     PHFCYRFDLAPKAGGMQPLNDFALASRLSYFLWSSMPDAELMSHAAAGDLRQPEVLLG
                     QTRRMLRDPKVRGLAVEFGGNWLDFRRFEEHNAVDRERFPSFDSDLRQAMFEEPVRLL
                     TDALQNNRSVLDVL"
     gene            2325..>4008
                     /locus_tag="VFB80_04375"
     CDS             2325..>4008
                     /locus_tag="VFB80_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33026.1"
                     /translation="MKGPLLRFAPALVAAVTAVMGVRAAEPPDFEREIAPLLINHCLG
                     CHQPNKRSGGLDFSSRASLIAGGESGPALSPGKPEDSLLHQRASSREMPPPEAKESQP
                     LSAAEVALLKTWIAAGASWPAERVLGIHERTVDLAAAREFWSFQPVNRPPIPIVKNQG
                     RVSNPIDAFVWEKLEAAGLEMSPPAAPADLLRRLSLDLHGLPPPADLASGRREPPDDN
                     SLPARSERYREADASRSPLLIEYELLVDQLLADPAYGERWARHWLDLVRYADSNGYER
                     DDAKPSVWRYRDYVIAALNSDKPFDRFVLEQIAGDELPDASDETLIATGFHALGAWQD
                     EVDPLEAAQYRADELDDMVRTTSQTFLGLTLGCARCHNHKFDPLTMVDYYSLVAILAP
                     LKRPNKGRLDRDIPLGTPEQVAALNARNEELGKLDAKVLEIITKKPADAKEQQAAIRQ
                     RQQELRAQTPDRPGAYRCFEDSPEAPPTYLLLSGRASNPGPLMQPRMPAVLVGNALRG
                     VPAGQPEFPAPAAKSTLRRLTLARWIASEANPLTARVIVNRVWQHHFGTGLVA"
BASE COUNT          675 a         1394 c         1271 g          668 t
ORIGIN      
        1 tcgagcacgt ccagcaccga gcggttgttc tgcagggcgt cggtcaatag ccgcaccggc
       61 tcctcgaaca tcgcctgtcg caggtcgctg tcgaaactgg ggaaccgctc gcgatccacc
      121 gcgttgtgct cttcgaagcg gcggaagtcg agccagttcc cgccgaattc gaccgccagt
      181 ccgcggactt ttggatcgcg cagcatccgc cgcgtctgcc cgagcagcac ctccggctgc
      241 cgcaaatcgc ccgcggcggc gtgggacatt agctccgcat cgggcatgct cgaccaaagg
      301 aagtagctca gccggctggc cagcgcgaaa tcgttcagcg gctgcatgcc accggctttc
      361 ggcgcgagat cgaagcggta gcagaaatgt ggtgacagca aaacgctggc cagcgtgtcg
      421 cgaaaggcct cctcgtgcgg caggcggtcc ttctcgcgga ggcggcgata gaacgcgagc
      481 aactcctcgc gctccgccgc agtgagcggc cgccgatagg cccgctcggc aaaatcggcc
      541 agcgcctgca aatggctcgg ctcggcggct agccgcgccc gctcgaccca gcggatttca
      601 gcctcgatgt tcgcgtaata cgtctcgatc gctgccaggg cgtccggtcc ggcgcctttc
      661 tttttggcca gcgccaggtg cagctcttgc agccgccgca tcttctcggg cgacgcggcg
      721 tccttgtctt ccgagcgggc gaaatcaaac tgggcgtcat acatgaaccg cggcggctcg
      781 gcccgctcga agaagatgaa gtcctgatac tgccgcagcg gcacctgcgt gatgaagttc
      841 agctcctgcc acaggccgtc gagctcccgc cgctcggcat ccgtgagaat caagtcagcg
      901 agcggctgat cgtctcggta gtagccctgc atcaagtgaa aaccggccgt cagaaatcgg
      961 cctttcccgg cctgcttggg atcgaaatac ggcccgcggt cggaaacgac gaaggcatcg
     1021 ggaatgccct cgcaaaattg ctcgcaggcc gccgcgaatt gcttcaccgc agccgcgtcg
     1081 gacggatcaa ccgcgaagac cgccgccagc ttatgccccg tgccgacgta tttttggcgc
     1141 aatcgctcgg cgtcggcggc cgcattaccc aggtatttcg tgtgccgctc ggcgccctgc
     1201 cggttccgcc acaaaacgaa cggctgcgtg ccgtcggaga tgcccttcac gctcagtttc
     1261 ggcggctcgg ttgccagctc tttccgcagc cagagcgcct cgacgcgcag actctcgcat
     1321 tcctcctgcg gcgcggcagc atcgcggctc gccggcgcgg gcagctcttg ccattgcgtg
     1381 cgaatcgcag ccactggccc ggccgccggc aattccgcat cggtcagcag ccgccagatg
     1441 cgctccaggt acttgccgct caagcgggcg tccgcggcga cgtcggccag cctctgttcc
     1501 ggccggccca aggccgcccg atgccgatag cgccaggcgg ccaggaaata gtcggccaga
     1561 tcgacttcat gccgcttgta gaactcgatg atccggtgga cgcagtactt gtcgcggtcg
     1621 gtttcggtca ctgcggcgtg cggtgcaaag cgaaaaccct ctggcaagag caccgcgtga
     1681 tcggccactt gccgtgcggc ggcgaggtac ttctgcaaca gggccggcga catggccagc
     1741 gattctcccg agttgtcgaa gccggcctcg ttggccggat cgaccgggaa ctccttcgtc
     1801 ggccggatat cgactcccgt caggtcgcgg atcgtgtagt caaactcggc gctgttcagc
     1861 cggcgggcca gcacttcgcc cggatcgccg gcgtgccgct cagcctcgaa ttcacgaaag
     1921 gcgtcgatcc aagccagcag cgtcttgcgg tcgccatcgg ccagcttccg cgcggcctct
     1981 tccggcggca tctcaccggc ggccaaccgc tgccggacca tctcccacac gcggtggtcc
     2041 ttgccgaccg cggccgcgct cgtgaaccgg ctgagcttca gcttcccttc ctgcttcgtt
     2101 tcgccgtggc agccgtggca atgggtcttg accagcggcc cgatcgtctc ggcaaaccgc
     2161 tgggccagct ccgcctcggc gtcttccgcc gccagcggcg cagccagcag cgaagccagc
     2221 agcgccgcga ccaaccagcc tgaaatcgca cccagggacc gcatatttgc aatgatagcc
     2281 gactgcggat ggattggcga ttcgcagttg gtatgctggg ttcgatgaag ggccctctcc
     2341 tgcgctttgc gccggcactg gtcgcggctg tgacggctgt gatgggagtc cgtgccgccg
     2401 agccgcctga ttttgagcgc gaaattgctc cgctgctcat caaccactgc ctgggctgcc
     2461 atcagccgaa caagcgcagc ggcgggctgg atttctcgtc gcgggccagc ctgatcgccg
     2521 gcggcgaatc ggggccggcg ctttcgccgg gcaagccgga ggacagcctg ctgcaccagc
     2581 gcgcgagcag ccgcgaaatg ccgccgccag aggcgaaaga gtcgcagccg ctgtcggccg
     2641 cggaagtcgc gctgctcaaa acctggatcg ccgccggggc aagctggccc gcggagcgcg
     2701 tgctgggcat tcacgaacgc acggtcgatc tggccgccgc ccgcgagttc tggtcgtttc
     2761 agccagtcaa tcgtccgccg attccgatcg tcaaaaacca aggccgcgtt tccaatccga
     2821 tcgacgcctt cgtctgggag aagctggaag ccgcaggcct ggaaatgtcg ccccccgccg
     2881 cgccggccga cttgctgcgg cggctgagtt tggacttgca tggcctgccg ccgccggccg
     2941 acctggcgag cgggaggcgt gagcctcccg atgacaacag cttgccggcc cgcagtgaac
     3001 gctatcggga ggctgacgcc tcccgctcgc cgctattgat tgaatacgag ttactcgtcg
     3061 atcagctcct tgccgatccc gcttacggcg agcgctgggc ccggcactgg ctcgacctgg
     3121 tgcggtacgc cgacagcaac ggctatgagc gcgacgatgc caagccgagc gtctggcggt
     3181 atcgcgacta cgtgatcgcg gcgctcaaca gcgacaagcc gttcgaccgg ttcgtgctgg
     3241 agcagattgc cggcgacgag ctgccggacg ccagcgatga aacgctgatc gccaccggct
     3301 ttcacgctct gggagcctgg caggacgagg tcgatccgct cgaagcggcg cagtaccggg
     3361 ccgacgagct ggacgacatg gtccgcacga cgtcgcagac attcctcggc ctgacgctcg
     3421 gctgtgcccg ctgccacaac cacaagttcg acccgctgac gatggtcgat tactacagcc
     3481 tcgtggcgat cctcgcgccg ctgaagcggc cgaacaaggg ccgcctcgac cgcgacattc
     3541 cgcttggcac gcccgagcag gtcgccgcgc tcaatgcccg caacgaagag ctgggcaagc
     3601 tcgacgccaa ggtcctggaa atcatcacca aaaaacctgc cgatgccaaa gagcagcaag
     3661 cggcgatccg ccagcggcaa caggaattgc gggcccagac gccggaccgg cctggcgctt
     3721 accgctgctt cgaagacagc cccgaagctc cgcccacgta tttgctgctc tccggccggg
     3781 ccagcaatcc cggcccgctc atgcagccgc gaatgccggc ggtgcttgta gggaacgccc
     3841 tccgcggcgt tccggctggc caacccgaat ttcccgcgcc cgctgcgaaa tccaccctgc
     3901 gacgcctgac gctggcccgc tggatcgcca gcgaagccaa tccgctgacg gcgcgggtca
     3961 tcgtcaaccg cgtctggcag caccattttg gcacggggct ggtcgcca
//
LOCUS       DASRJB010000155         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3145905, whole genome shotgun sequence.
ACCESSION   DASRJB010000155 DASRJB010000000
VERSION     DASRJB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3145905"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2365
                     /locus_tag="VFB80_04380"
     CDS             <1..2365
                     /locus_tag="VFB80_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33027.1"
                     /translation="TVGLTLALWPASRPAYMRQLAQAFAKFDAGDAKEARRLAADLLS
                     NPNARFDDQGGAYFILGALTVAEADQQISAEKRRLLFLVAARYLDEARIRGVPQTRDR
                     QALWLHCRALHDGGRYARSVPVLREALAADSNPDPRLHALLADSYLRLAPPRLAEALA
                     HNRQYLAAAGAQLSPLDQDAAQLIAGQILLAQKDYAAAAETADRIQGASELKPAAIVL
                     RGRAQLALLKPPSGDPAKRQALVDELRLLSAREGLPPAVLAGAQLLLGLLYEQQGDDR
                     AAQAQLDRTRRAFFGQPEALAATVILADLVRRENPEQAVALYQRAIGQAGVAESYDNV
                     WLPAEELQKRLEQAIDDLADRGHLAEARELAGALVPLFPEPTALLRQAAIDRAWARHL
                     EARARTENSLAQETTLAEARQHWRAAGVLTRKLADQRITTRFYLEDLTRAAEDLRRGQ
                     GFEQAAAVYADLLRQDPREGEPEALVGSGESLLALGRIDESLAALVHCLQIYPRHPMA
                     YQARILASQAHQEKGELAPARELLIDNLYRFSLAPQSAEWRDSLFALGSLLYRQGVEL
                     EAKSRQAGVDRPDPAGRKDGLALLEQSHAAFDEALRTLSEAVQRYPTLPQAAVARYRM
                     AEAHRQSAKWPRKRLPTVTIETSRAALVRQMHEQLQLAVTDYSTLITQLSEQPDATRS
                     PIEGAILRNCYFGRADALFDQGNYEEAIQAYSAATNRYQHDPESLEAYVQIASCYRRL
                     GRAGEARGTLEQARIVLARIRADADFLRTTRLTRQDWTQLLDWLRTL"
     gene            2375..2848
                     /locus_tag="VFB80_04385"
     CDS             2375..2848
                     /locus_tag="VFB80_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33028.1"
                     /translation="MPTPATPAVSPTGTERLAALIAAKRQVLELLTRLSRRQLELIAG
                     GDTPALLKLLTAKDKVLAQLQLLERQLDPFRSENPDERVWSSPAARTACQQEADRASA
                     LLAEAMQLERQAEAAMIGRRDAAAAAVASAQVASDAAAAYAAGAISSPASLQVEG"
     gene            2859..3761
                     /locus_tag="VFB80_04390"
     CDS             2859..3761
                     /locus_tag="VFB80_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN33029.1"
                     /translation="MDEPTILKFPTRELSAAPLTDRSAPPPADQLDLQTILAAWNEAT
                     DRLQRTHETLRSEVSRLSDELEAKNRELARKNRLADLGQIASHVAHEVRNSLVPMKLY
                     LSLLRRRLAGDGGSLDVLDNVLSGFTALEATVGDLLHFSSQRDPQRRPVSMSVLLAEV
                     CDSLAPQLAAQNIRAAIDCPPDLVWEADGDMLRRALLNLVLNALDVLPAGGEIVLTAV
                     ATSRGLEVEVADSGPGIPPPLIDRLFEPFFTTKPSGTGLGLAIVERIAAAHGGQISAA
                     NCPEGGAAFTLQLPRASRALEKAA"
     gene            3758..>4644
                     /locus_tag="VFB80_04395"
     CDS             3758..>4644
                     /locus_tag="VFB80_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN33030.1"
                     /translation="MTATIAHSGSWTARKPAARPLAAAPAIPELTGRVLVVDDNARAR
                     QSMVEVLSAAGHDVAAAAGAIEALRLVVEQDFDLIITDLQMPGMDGLAFIRTLAERKS
                     ESQIVMVTAFASVASAVEAMRYGAFDYIEKPFDVEQLESLVARALRHGAKLGRRSSVP
                     PPAADWAGIMVGASPAMQQLHRRIAQIAPTDETVLITGESGTGKELVARCLHAASRRS
                     GRALVGLNCPALSPQLMESELFGHERGAFTSADAPRVGRFELADGGTILLDEVTEIDM
                     PLQAKLLRVLQERTYERVGS"
BASE COUNT          746 a         1623 c         1582 g          693 t
ORIGIN      
        1 cacggtgggg ctgactttag cgctgtggcc cgcgagccgg ccggcctaca tgcggcagct
       61 ggcccaagcg ttcgcgaaat tcgacgcagg ggatgcgaag gaggcccgca ggctggctgc
      121 cgacttgctc tccaatccca atgcccgctt cgacgaccag ggaggcgcct atttcatcct
      181 cggagcactg acggtggccg aggccgatca gcagattagc gccgagaagc ggcggctctt
      241 gttcctcgtg gccgcccgct atctggacga agctcgcatc cgcggcgtgc ctcagacgcg
      301 cgatcgccag gctttgtggc tgcactgccg cgcgctgcac gacggcggcc gctacgcgcg
      361 aagcgttcca gtgctccgcg aggccctggc ggctgattcg aatcccgatc cgcgtctgca
      421 cgccctgttg gccgacagtt acttgcgtct ggcgccgccg cgactggccg aggcgctcgc
      481 gcacaatcgg cagtacctgg ccgcggcggg agcgcagctc tcgccgctgg accaggacgc
      541 ggcgcaattg attgcgggac agattcttct cgcccagaaa gattatgccg cggcggcgga
      601 aacggccgac cgcattcagg gtgcgtcgga gctcaagccc gccgcaatcg tcctgcgagg
      661 ccgggctcag ctggccctgc tcaagccgcc ctccggcgat cccgccaagc gccaggcgct
      721 cgtggacgag ttgcggctgc ttagcgctcg cgaagggctt cctccggccg tcctggccgg
      781 cgcgcagctg ctgttgggac tcttgtacga gcagcaaggc gacgaccgcg cggcccaggc
      841 gcagctggac cgcacgcggc gggccttctt tggacagccg gaagccctgg cggcgacggt
      901 gatcctggcc gacctggtgc ggcgagagaa tccggagcag gccgtggctc tctatcagcg
      961 ggcgatcggt caggccggag tggccgagtc ttacgacaac gtctggctcc ctgccgagga
     1021 gctgcaaaag cggctggagc aggccatcga cgacctggcc gaccgcgggc atcttgcgga
     1081 ggcgcgtgag ctggccggtg cactggtgcc gctctttccc gaaccgaccg ctttgctacg
     1141 gcaggccgcg atcgatcggg cctgggcgcg gcacctcgaa gcgcgggcga ggaccgaaaa
     1201 ctccctggcg caggaaacga cgctggcgga agcccgccag cattggcgag cggccggcgt
     1261 cctgacccgc aagctcgccg atcaaaggat cacgacgcgg ttctacctgg aggacctgac
     1321 ccgcgcggcc gaggatttgc gccgcggtca aggtttcgaa caggcggctg cggtctacgc
     1381 cgatctgctg cggcaagacc cgcgcgaagg cgagcccgag gccctcgtcg gcagcgggga
     1441 atcgctgctg gcgctcgggc gcatcgacga gtcgctggcc gcactcgtgc actgcctgca
     1501 gatctatccg cggcatccga tggcctatca ggcccgcatc ctggcgagcc aggcccatca
     1561 ggaaaaggga gagctggctc cggccaggga gctgctaatc gacaacctgt accgcttttc
     1621 actcgctccg cagagtgccg agtggcgcga ctcgctgttc gcgctcggga gcttgctgta
     1681 tcgccaaggc gtggagttgg aagccaagag ccggcaagcc ggcgtcgatc ggcccgatcc
     1741 ggcgggccgc aaggacggcc tcgcgctcct ggagcaaagc catgcggcct tcgacgaggc
     1801 gctgcggacg ctgagtgagg ccgtgcagcg ctatccgacg ctgccgcaag ccgcggtggc
     1861 ccgctatcgc atggccgagg cccaccgcca gagcgcgaag tggccccgca agcgcctgcc
     1921 gaccgtgacg atcgaaactt cgcgggcggc cctcgtgcgg cagatgcacg agcagctgca
     1981 actggcggtg accgactaca gcacgctgat cacgcagctc agcgagcagc ccgacgccac
     2041 gcgctcgccg atcgagggcg cgatcctgcg gaattgctac ttcggccggg ccgacgctct
     2101 gttcgaccag ggcaactacg aggaggcgat ccaggcctac tcggcggcca ccaaccgcta
     2161 ccagcacgac cccgaatcgc tcgaagccta cgtgcagatc gccagctgct accgccgctt
     2221 ggggcgggcc ggcgaggccc gcggcacgct cgaacaggcc cgcatcgtct tggcccgcat
     2281 ccgcgcggac gccgatttcc tccgcaccac gcgactgacg cggcaggatt ggacgcagct
     2341 tttggattgg ctccgcaccc tgtaactcca gaccatgccg acgcccgcta cacctgcagt
     2401 ttcgccgact gggaccgagc gtctggccgc gctcatcgcg gccaagcgcc aggtcctgga
     2461 attgctgacg cgcctgtcgc ggcggcagct cgagctgatc gccggcggcg acacgccggc
     2521 gctgctcaag ctgctgacgg ccaaggacaa ggtgctggcc cagctgcagc ttttggagcg
     2581 gcagctcgat ccgtttcgca gcgaaaaccc tgatgagcga gtctggtcgt cgccggcggc
     2641 gcggacagcc tgccagcagg aggccgatcg tgccagcgcc ctgctggcgg aagccatgca
     2701 gctggaacgc caggccgaag cggccatgat cggccggcgc gacgccgccg cagcagccgt
     2761 ggcgtcggct caagttgcca gcgatgcagc cgcggcgtat gccgccggcg cgatttccag
     2821 tccggccagc ttgcaagtcg aaggatagga gcccaggcgt ggacgaaccg accatcttga
     2881 aatttccgac ccgcgagctt tccgccgcgc cgctaacgga ccggtccgcg ccgccgccag
     2941 ccgaccagct cgacttgcag acgatcctcg cggcctggaa tgaagcgacc gaccgtttgc
     3001 agcggacgca cgaaacgctc cggtccgagg tcagccgact gtcggacgag ctggaagcca
     3061 agaaccgcga gctggcccgc aaaaaccgcc tcgcggactt ggggcagatc gcctcgcacg
     3121 tggcccacga agtccgcaac agcctggtgc cgatgaaact ctacctcagc ttgctgcgcc
     3181 gccggctcgc cggcgacggc ggcagcttgg acgtgctgga caatgtgctc tcgggcttca
     3241 ccgccctgga ggcgacggtc ggcgatctgc tgcacttctc cagccagcgc gatccgcagc
     3301 ggaggccggt ctcgatgtcc gtgctcttgg ccgaagtctg cgattcgctg gccccgcagt
     3361 tggccgcgca gaacattcgc gcggccatcg attgcccgcc ggacctcgtt tgggaggccg
     3421 acggcgacat gttgcggcgg gcgctgctca atctagtgct gaacgcactc gatgtgctgc
     3481 ccgcgggcgg cgaaatcgtc cttacggctg tggccacttc ccgcggactg gaagtcgagg
     3541 tcgccgacag cggcccggga attcccccgc cgctcatcga ccgcctgttc gagcccttct
     3601 tcacgaccaa acccagcggc acggggctgg gcctggcgat cgtcgaacga atcgccgccg
     3661 cgcatggcgg ccagatcagc gctgcgaatt gccccgaagg gggcgcagcc ttcacgttac
     3721 aactgccgcg cgcgtcgcgc gcgctggaga aagccgcatg accgcaacca ttgcccattc
     3781 cggttcgtgg actgctcgca agcctgcggc gcgtccgctg gccgccgcac ccgccattcc
     3841 cgagctcacc ggccgcgtgc tcgtggttga cgacaacgcc cgcgcccgcc agtcgatggt
     3901 cgaagtgctc tcggccgccg gccacgacgt ggccgcggcg gccggcgcga tcgaagcgct
     3961 gcggctggtc gtcgagcagg atttcgatct gatcatcacc gacctgcaaa tgcccgggat
     4021 ggacggcctg gcgttcatcc gcaccctcgc cgagcggaag agcgaatcgc agatcgtgat
     4081 ggtcacggcg tttgcctcgg tcgcctccgc cgtcgaggcg atgcggtacg gcgcattcga
     4141 ttacatcgag aagccgttcg acgtcgagca gctggaatcg ctggtggccc gggccctccg
     4201 ccacggcgcg aagctgggcc gccgctcgag cgttccgccg cccgcagccg actgggcagg
     4261 gatcatggtg ggcgcgagcc cggcgatgca gcagctccac cgccgcatcg cgcagattgc
     4321 gccgaccgat gagaccgtcc tgattacggg cgaaagcggc acgggcaagg agctggtcgc
     4381 gcggtgcctg catgctgcca gccgccgcag cggccgggca ctggtcgggc tgaactgccc
     4441 ggcgctgtcg ccgcagctca tggagagtga gctgttcggc cacgagcgcg gggcgttcac
     4501 cagcgccgac gctccccgcg tcgggcgctt cgagctggcc gacggcggca cgatcctgct
     4561 cgacgaagtc accgagatcg acatgccgct ccaggccaag ctgctgcgcg tgctccagga
     4621 gcggacctac gagcgcgtcg gctc
//
LOCUS       DASRJB010000156         8277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_326554, whole genome shotgun sequence.
ACCESSION   DASRJB010000156 DASRJB010000000
VERSION     DASRJB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326554"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFB80_04400"
     CDS             complement(<1..870)
                     /locus_tag="VFB80_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZN33031.1"
                     /translation="MVGSSSKRRNRARQFWTATLCAAGFVTGQWLFGQQPAPSAGTEP
                     TPQSITNPFLPARSASSARPSAAGPRPVEIEPVSDVPAAAPASVEQPRLVEARPLVVL
                     NPTVSQPAQKVAQTSTSQPQKKPAVAQAIDQEETLAPIVPAWAMPAKGNRAPASKIQK
                     AAMTAADAPRELIPDAEASVISAPRRGVATAEFDALQPAAQPPAPDASGILPPGSPFE
                     AIPQSGEILLRVRRSLLLRTKVDVYRTAIVDEAVCDIVQFTPREVSIIGRSLGQTHVT
                     FWFDDPATPPLTYL"
     gene            1257..2696
                     /locus_tag="VFB80_04405"
     CDS             1257..2696
                     /locus_tag="VFB80_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33032.1"
                     /translation="MTATWSWAQLPPSAVRSTADVRPRIPDEHDRPYNRETQSHRGWE
                     IREPQYTVFATTSPDDARWAASHVAAAWNNASRLAGRWTAVQQNPDFGLSSLQVVIDG
                     EPLRDRDSPLTTINVVGIQTQVQLNVAPGQPPLKQQLVRLREGTALAMLHTAGLDSAA
                     PPWVVAGIAAASGRQGLTDEQLRAEAASPSTAHFGGQQWRFAREGQDVLAYRRLDHDA
                     AAAQVAFLLTGSDAQHAPEFLSALNQATREAARTAAEGGAFRALPGDRQPAGADTPFD
                     SLLAERHTQFKEWQQEPLSGQPVFEAPADADPELIAAEREMLVLLKLQRRLAATSSDP
                     QRSFAAGVPRTKVVTFDPAKGASSRPGRSKPTIATFSDLAARLSDPAQGVWATLDVDG
                     SLLLSTDTERVQALLATAENRYSLETSSGRTTLVRQLDGNKALRGWLAENTEKKSRPL
                     AKFEVASAGQKRPANAADQAPRQVRFEQR"
     gene            complement(2693..3460)
                     /locus_tag="VFB80_04410"
     CDS             complement(2693..3460)
                     /locus_tag="VFB80_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN33033.1"
                     /translation="MARRAIDGLRVVVTGGSSGIGRALVLELIRQGASVVALARRADR
                     LQELGALVADEQRYRWLAGDVTRREDRAAALALAQREFGGLDALVNNAGIGALGRFDA
                     ADEGRLRRIFEVNFFAAAEFTREALPLLRAGNKPIVVNIGSVLGHRAVPEKSEYCASK
                     FALHGLSDALRIELAPLGIDVLLVSPSTTQSEFFESAGAKQTRGRGMPAETVARRTVA
                     AMRAGRREIILSAGGNLLVWLDRLCPPLADWLIARWA"
     gene            complement(3463..4755)
                     /gene="hflX"
                     /locus_tag="VFB80_04415"
     CDS             complement(3463..4755)
                     /gene="hflX"
                     /locus_tag="VFB80_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZN33034.1"
                     /translation="MRVILNGQAAGDDPLDEITGLAQTAGAQVVSGLVQRRDVPDVTT
                     YLGKGKVDELKSLVAHHDADVVLFDNELAPSQTRNLEKAVGVKVLDRTELILDIFAGS
                     ARTYEARLAVELAQLEYSLPRLKRMWTHLSRIKAGIGMRGPGEKQIEEDRRLVEKRIH
                     ELRSELYQIERRKERLVASRGDRLTVSLVGYTNAGKSTLMNALTGAGVYAADKLFATL
                     DTRTRKWMLPSWGPVLLSDTVGFIRDLPHGLIASFKATLEEARQADLLLHVADAANPA
                     VLDQISAVYEVLRELGIEEKDTLLVLNQIDRLADRSELAKVQQRYPNAVPISARTGEG
                     LDKLHAAASAALSRSFVDLDVETSVANGKLLAYLAAHGEILDRKYDGDRALIHVRIPQ
                     RHLGPLYQSGTTFRPHPQFAAVPDGEAERAAAPIEEVA"
     gene            complement(4902..5123)
                     /locus_tag="VFB80_04420"
     CDS             complement(4902..5123)
                     /locus_tag="VFB80_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HZN33035.1"
                     /translation="MPARFEYAFVRLEQDKNWWTGLPGPSMEAREGYQKVVRQHAAEG
                     WRLVQIFAPDLGFHGSALYFELIFERPLD"
     gene            complement(5133..5747)
                     /locus_tag="VFB80_04425"
     CDS             complement(5133..5747)
                     /locus_tag="VFB80_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN33036.1"
                     /translation="MSQTPTAAEIAAIDAALRLIDREIWIVTAAAGGRRGGLVATWVS
                     SASIDRERPVLMAGIAPNHFTAELIDASGALAAHLLRPDQVELAWNFARVSGRHCDKL
                     AGLATNNSQSGPPILADCLASFDCRVFARYDAGDRLFYWADVVAASAAGAPLAALREQ
                     AFIRALSDEQRQRLAADRESDVVRQRPGHEKWRFANLSSARPSK"
     gene            complement(5755..>8277)
                     /locus_tag="VFB80_04430"
     CDS             complement(5755..>8277)
                     /locus_tag="VFB80_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN33037.1"
                     /translation="ADGLIRDVTREAFIESGNIEIIEANTSGVLTTLRRGEAPVLVRY
                     EGAYAATTIVCMGDRTGFAWQPQPVHNYIDPLVDKKLQAVKVLPSELCTDEEFVRRVY
                     LDLTGLAPSAAQTREFLANPAPSKAKREALVDQLIGSREYVEHWTNKWADMLQVNRKF
                     LGEEGSAALRNWIKDRIAQNQPYDQFARDVITASGSNMENPPAAYWKVNRDPAAAMEN
                     TTHLFLAVRFNCNKCHDHPFERWTQDQYYHLAQYFAQVSRKEDATFSGQKVGGSAVEG
                     AVPLVEVVYDGTSGDVKHDRTGQVVAPSFPYTHADLSPATAPRREQLAHWITSKDNQY
                     FAKSYVNRLWGYLFGVGIIEPIDDIRAGNPPTNPELLDALTKDFIASGFDAQHILRTI
                     CKSRTYQLSVVSNKWNEDDTINYSHAIPRRLPAEALYDAIFLATGATERLPGVPAGFR
                     AAQLPDAGASVPFLDDFGKPVRETACECERSSGMVLGPILKLINGPTVADALADPASE
                     LNQLVASQPDDAKLIEEVFVRFLARKPSATELKLGVEALQAAAADQAKAAAALAEYEK
                     QLPAKQVAWEASFGKPVVWQPLDASETKSAVGATFAKQEDKSIVVTGNLEKDVYTIVA
                     KTNLKGITGIKLEALNDASLAAGGPGRAANGNFVVSEFKVSLAAAGAGESAPVELAGA
                     SADFNQDGWHVSGAIDGSDQTGWAVSPEFGKPHEAIFETKADAGGDGGSTLTITISQQ
                     YPDGKHCLGKFRLSVTDGARPLTKAKLPGLVAAALAVPKDQRTPDQAAMIAAHYKSLD
                     AEHARLSAEVQRTAEQAKSARAIGVQDLGWALINNPAFLFNR"
BASE COUNT         1326 a         2744 c         2791 g         1416 t
ORIGIN      
        1 caggtacgtc agcggcggcg tggccgggtc gtcgaaccag aaggtgacgt gggtctggcc
       61 cagcgagcgg ccgatgatcg agacctcgcg gggcgtgaac tgcacgatgt cgcagaccgc
      121 ttcatccacg atcgccgtgc gatacacatc caccttcgtc cgcaacaaca gcgaccgccg
      181 cacccgcagc aggatctccc ccgactgcgg aatcgcctca aacggcgacc ccggcggcag
      241 aatcccagac gcgtccggag cgggcggctg tgctgcgggc tgcaaggcgt caaactctgc
      301 cgtcgccacg ccgcggcgcg gcgcactgat cacgctggct tccgcgtccg gaatcagctc
      361 acgtggcgca tccgcagccg tcatcgcagc cttttggatt ttactagcgg gagctcgatt
      421 gcctttcgcc ggcatggccc acgccggcac gatgggtgcg agcgtctctt cctggtcgat
      481 tgcctgggcc accgccggtt tcttctgcgg ctggctggtg gaggtttgtg ccaccttctg
      541 tgccggctgc gaaaccgtgg ggttgagcac gacgagcggc cgggcctcga cgaggcgcgg
      601 ctgctctaca ctggccggcg ccgcggcagg cacatcgctc accggttcga tctcgacggg
      661 ccgcggcccg gcggcgctgg gacgggcgct actggccgac ctggccggca agaacggatt
      721 ggtaatcgat tgcggcgtgg gctccgtgcc cgcggagggc gctggctgct ggccgaacag
      781 ccattgtccc gtgacaaagc cggcggcaca aagcgtggcg gtccagaact gtcgcgcgcg
      841 gttgcggcgc ttcgatgacg aacccaccat gaagtccccc cttggcgatg ccgaatcgct
      901 gcggctggca gcgaggcgac acgctatctg cggtgtattc cagcgggcca gggtgcgcgc
      961 cgcatacggc acgaacccgt accctgtctg ttcgtatcgg gactgccgca cgatccggtt
     1021 gaggaaaatg tgcgggtaat tcgcattctg cccggcgtac cgatcgcacc ggaattgccg
     1081 cggataccgt ccgagtcagc gccgtacaat ccgaataatc ggctcagccg gcctgtagcg
     1141 acccgaggga gacgagccga tacacttcgg gacactccca ctgcgccact ggatgcgagg
     1201 aacgcatgtc acgactagca aggcgaacgg tgctggcaag ctgcgggcta gctctggtga
     1261 cagcgacttg gtcgtgggca cagctgccgc cgtcggcggt ccgcagcacg gccgacgtcc
     1321 ggccgcgaat ccccgacgag cacgaccggc cctataacag agagacccag tcgcaccgcg
     1381 gctgggaaat ccgcgagccg caatacacgg tcttcgcgac gaccagcccg gacgacgccc
     1441 gctgggccgc gtcgcacgtc gccgcggcct ggaacaatgc ctcgcggctg gcgggccgct
     1501 ggaccgcagt tcagcagaac ccggatttcg gactgagcag cctgcaagtc gtcatcgatg
     1561 gtgagccgct ccgcgatcgc gactccccgc tgacgacgat caacgtcgtc ggcatccaga
     1621 cacaagtgca gctcaacgtc gcgccgggcc agccgccgct gaagcagcaa ctcgtgcggc
     1681 tgcgggaagg gacggcgctg gcgatgctgc acaccgccgg gctcgattcg gccgctccgc
     1741 cgtgggtcgt ggcgggaatc gccgctgcgt cgggaaggca ggggctgacc gacgagcaac
     1801 ttcgagccga ggctgcttct ccgtccacgg ctcatttcgg cggccagcag tggcggtttg
     1861 cgcgcgaggg tcaggacgtc ctggcctacc ggcggctgga ccatgacgcg gccgccgccc
     1921 aggtggcgtt tctgctgacg ggcagcgacg cacagcatgc gcccgaattc ctgtctgcgc
     1981 tgaaccaggc gacccgcgag gcggctcgca cggccgcgga gggtggcgcg tttcgcgcgc
     2041 tgccgggcga caggcagccc gccggcgccg acactccgtt cgactcgctg ctcgccgagc
     2101 gccacaccca gttcaaagag tggcagcagg agccgctgtc cgggcaaccc gtgtttgagg
     2161 ccccggcgga cgccgatccc gagttgattg cggccgagag ggaaatgctg gtgctgctga
     2221 agctccagcg gcgactggca gcaacttcga gtgatcccca gaggagtttc gcggccggcg
     2281 tcccgcgcac caaagtcgtt acgttcgacc ccgccaaagg ggcctcctcg cggcccggcc
     2341 gctccaagcc gacgattgcc actttctcgg atctggccgc gcggctgagc gatccggcgc
     2401 aaggggtctg ggcgacactc gatgtggatg gcagcctgct gctgtccacc gacacggagc
     2461 gggtgcaggc actgctcgcg accgccgaga accggtattc gctggaaacc agcagcggac
     2521 ggaccacact cgttcgccaa ctggatggaa acaaggctct gcgcggctgg ctggccgaga
     2581 ataccgagaa gaagtcgcgt ccgctggcga agttcgaggt cgcgagcgcc gggcaaaagc
     2641 gtcccgccaa tgccgctgac caggcgccgc ggcaagtccg cttcgagcag cgctaggccc
     2701 agcgggcgat gagccagtcg gccaggggcg ggcagaggcg gtcgagccaa acgagcaagt
     2761 tgccgccggc ggagagaatg atctcgcggc ggccggcccg catcgccgcc accgtacggc
     2821 gggcgacggt ctcggccggc atgccgcgcc ctcgcgtctg tttcgctccc gcggattcga
     2881 agaactcgct ctgggtcgtg ctgggactga cgagcagcac gtcgattccc agcggcgcaa
     2941 gctcgatccg cagcgcgtcg gacaggccgt gcagcgcaaa cttgctggcg cagtactcgc
     3001 tcttctccgg aacggcccgg tggcccagca cggagccaat attgacgacg atcggcttat
     3061 tgccggcgcg gagcagcggc agcgcctcgc gggtgaattc cgcagccgcg aagaagttca
     3121 cctcgaaaat ccgccgcagg cggccttcgt ccgccgcgtc gaatcgcccc agggcgccaa
     3181 tgccggcgtt gttcaccagc gcgtcgaggc cgccgaattc gcgctgagcc agcgcgagtg
     3241 ccgctgcgcg gtcttcgcgc ctggtaacgt cgccggccaa ccagcgatag cgctgttcgt
     3301 cggcgacgag cgcgcctaac tcttgcagcc gatcggcccg ccgcgccagg gcgacgacgc
     3361 tcgcgccctg gcgaatcaat tccagtacga gagctcggcc gatgccgctg gagcccccgg
     3421 tcaccaccac gcgcagcccg tcgattgcgc gccgcgccat cgctaggcca cttcttcgat
     3481 gggcgccgca gctcgctcgg cctcgccatc gggcacagcc gcgaattgcg gatgcggccg
     3541 gaacgtggtg ccgctctggt ataacggccc cagatgccgc tgcggaatcc gcacgtggat
     3601 cagggcccgg tcgccgtcgt acttccggtc gagaatctcg ccgtgtgcgg ccagatacgc
     3661 cagcagtttt ccgttagcga cgcttgtctc cacgtccaaa tccacgaacg accggctgag
     3721 cgctgcgctg gcggcggcat gcagcttgtc cagcccttcg ccggtgcgag cgctgatcgg
     3781 cacggcattg gggtagcgct gctggacctt ggccagctcg ctgcggtcgg cgagccggtc
     3841 gatctggttc agcaccagga gcgtgtcctt ctcctcgatg ccgagctcgc ggagcacctc
     3901 gtagacggcg ctgatctgat ccagcaccgc ggggttggcg gcgtcggcca cgtgcagcag
     3961 cagatccgcc tggcgggctt cctcgagcgt ggccttgaag ctggcaatca ggccgtgcgg
     4021 cagatcgcgg atgaagccga ccgtgtccga gagcagaacc ggcccccagc tcggcagcat
     4081 ccacttgcgg gtccgcgtgt cgagggtggc aaacagcttg tcggcagcgt acacgccggc
     4141 ccccgtcaag gcgttcatca gcgtgctctt gccggcgttg gtatagccga ccagcgagac
     4201 cgtcagccgg tcgccgcgcg aggcaacgag ccgctccttg cggcgctcga tctggtacag
     4261 ctcgctgcgc agctcgtgga tccgcttctc gacgaggcgg cggtcctcct cgatctgctt
     4321 ttcgccgggg ccgcgcatgc cgatcccagc cttgatgcgg gagaggtgcg tccacatccg
     4381 cttcagccgc ggtagcgagt actcgagctg ggccagttcg acggccaggc gggcttcgta
     4441 cgtgcgggcg ctgccggcga agatgtccag gatcagctcg gtgcggtcga gcaccttgac
     4501 gccgacggcc ttttccaggt tgcgcgtctg gctgggagcc agctcgttgt cgaagaggac
     4561 cacgtcggca tcgtggtggg caacgaggct cttcagctcg tcgaccttcc ccttgcccag
     4621 ataggttgtg acgtcgggaa cgtcccgccg ctgcaccagc ccgctgacaa cctgcgctcc
     4681 ggcggtctgg gccaggcccg tgatttcgtc cagcggatca tcgcccgcgg cctggccgtt
     4741 cagaatgacg cgcacgagaa cggccttctc gctcgctacg ccgtcggctc gttcgatttc
     4801 gtacactgtg cggggctgcc tccggggacg actcgaattt tgactgggcg gcggcgtgag
     4861 gggaagagaa gaagcggaca atccattcta ctccaggaat gttaatcgag cgggcgctcg
     4921 aaaatcagct cgaaatacag ggccgaaccg tgaaagccca ggtcgggggc aaaaatctgc
     4981 accagccgcc agccttcggc ggcatgctgg cggacgactt tctggtagcc ttcgcgggct
     5041 tccatcgatg gcccgggcaa gcccgtccac cagtttttat cctgctccag gcggacgaag
     5101 gcgtattcga agcgggctgg catgggtgct cctcacttag acggtcgggc ggaggaaagg
     5161 ttcgcgaagc gccatttttc gtggccagga cgctggcgga caacgtccga ctcgcggtct
     5221 gccgccaggc gctgccgctg ctcgtcgctc agcgcgcgaa tgaacgcctg ctcgcgcaag
     5281 gcagcaagcg gcgcgcctgc cgcgctggcc gcgacgacgt cggcccagta aaacagccgg
     5341 tccccggcat cgtagcgggc aaatacgcgg cagtcgaacg acgccaggca gtcggcgagg
     5401 atcggcggtc cgctttggct gttattcgtc gccaggccgg ccagcttgtc gcaatggcgg
     5461 ccgctgacgc gcgcgaaatt ccaggccagc tccacctggt cgggccgcag caggtgggca
     5521 gccaacgcac cgctggcatc gatcagctcg gccgtgaagt ggttcggcgc aatgccggcc
     5581 atcagcaccg gacgctcgcg gtcgatcgaa gcgctggaga cccaggtggc caccaggccg
     5641 ccgcgccgcc cgccggccgc ggccgtgacg atccagattt cgcggtcgat caaccgcagg
     5701 gcggcgtcga tcgcggcaat ctcggcggcg gtgggagtct ggctcaattc gcaactagcg
     5761 gttgaacaga aacgccggat tgttgatgag tgcccagccc aggtcctgta cgccgatcgc
     5821 gcgggcgctc ttggcttgct cggctgtccg ctgcacttcc gccgacaggc gggcatgttc
     5881 cgcgtcgagc gatttgtagt gggcggcgat catcgcggcc tggtcaggtg tccgctggtc
     5941 cttgggcacg gccagcgcgg cggcaacgag ccctggcagc tttgccttcg tcagcggccg
     6001 agcgccgtcg gtgacgctga ggcggaactt tcccaggcag tgcttgccgt cgggatattg
     6061 ctgtgaaatg gtgattgtca gcgtgctgcc gccatcgccg ccggcgtccg ccttggtctc
     6121 gaagatcgct tcgtgcggct tgccgaactc gggcgagacg gcccagccgg tctgatcgct
     6181 gccgtcgatc gcccccgaaa cgtgccagcc atcctggttg aagtcggccg atgcgcccgc
     6241 cagctcgacc ggcgcggatt ctcccgcgcc ggcagccgcg agcgacacct tgaattcgct
     6301 cacgacaaaa ttgccgttgg cggcgcggcc ggggccgccg gcggcaagcg aggcgtcgtt
     6361 caaggcctcc agtttgatcc cggtgatgcc cttgaggttc gtcttggcga cgatcgtata
     6421 gacgtctttc tccaggttgc ccgtaacgac gatcgatttg tcttcttgct tggcaaacgt
     6481 cgctccaact gcggatttcg tttcgctggc atcgagcggc tgccacacga caggcttgcc
     6541 gaacgacgcc tcccaggcga cttgtttggc cggaagctgc ttctcgtact cggccagggc
     6601 cgccgccgcc ttggcctgat cggcggccgc ggcctgcagc gcctccacgc ccagcttcag
     6661 ctccgtcgca ctcggcttgc gggcgaggaa ccgcacgaag acttcctcga tcaacttcgc
     6721 gtcatccggc tgcgaagcca cgagctggtt cagctcgctc gccgggtcgg ccagcgcgtc
     6781 agccacggtc gggccgttga tcagcttgag gatcggcccc agcaccatgc cgctggagcg
     6841 ctcgcactca caggccgtct cgcggaccgg cttgccgaag tcgtcgagga acggcaccga
     6901 cgcaccggca tcgggcagct gcgccgcgcg gaagccagcg ggaacgccgg gcagcctttc
     6961 ggtggcgccc gtcgccagga aaatcgcatc atacagggcc tccgccggca gccgacgggg
     7021 gatggcgtgc gaatagttga tcgtatcgtc ctcgttccat ttgttgctga cgaccgaaag
     7081 ctgatacgtc cgcgacttac agatcgtccg caggatgtgc tgggcgtcga agccgctggc
     7141 gatgaagtct ttggtcaagg cgtcgagcaa ctcgggattg gtcggcgggt tgccggcgcg
     7201 gatatcgtcg atcggctcga tgatgccgac gccgaacaaa tagccccaca gtcggttgac
     7261 gtagctcttg gcgaaatact ggttgtcctt ggacgtaatc cagtgcgcca attgctcgcg
     7321 gcgcggggca gtcgccggcg agaggtccgc gtgcgtgtag gggaacgaag gtgctactac
     7381 ctgccccgtg cgatcgtgtt tcacatcgcc gctcgtgccg tcgtaaacca cctcgacgag
     7441 cggcaccgct ccttcgactg cgctgccgcc gaccttctga ccgctgaacg tcgcgtcttc
     7501 cttgcggctg acctgagcga agtactgggc caggtgatag tactggtcct gggtccagcg
     7561 ctcgaacgga tggtcgtggc acttgttgca gttgaagcgc actgccagga agaggtgcgt
     7621 ggtgttttcc attgcggcgg ccgggtcgcg attgaccttc caataagcag cgggcggatt
     7681 ctccatgttc gagccgctgg cggtgatcac gtcgcgggca aattgatcgt agggctgatt
     7741 ctgcgcgatc cgatccttga tccaattcct cagggccgcc gagccttctt ctcccagaaa
     7801 cttacggttg acttgcagca tgtcggccca cttgttcgtc cagtgctcga cgtactcgcg
     7861 gctgccgatg agctgatcga cgagggcctc gcgcttggcc ttgctcggcg cgggattggc
     7921 caggaattcg cgggtctgcg ccgcgctggg ggccaagccc gtcaggtcga ggtacacgcg
     7981 gcggacgaac tcctcgtcgg tgcagagttc gctgggcagg accttgaccg cctggagctt
     8041 cttatcgaca agcggatcga tgtagttgtg caccggttgc ggctgccagg cgaagcctgt
     8101 gcggtcgccc atgcagacga tcgtcgtcgc ggcataagcg ccctcgtaac ggacgagcac
     8161 cggagcctcg ccgcggcgaa gcgtcgtgag tacgcccgag gtattagcct cgatgatctc
     8221 gatgttgccg ctctcgatga aggcttcgcg ggtgacgtcg cggatcagtc cgtcggc
//
LOCUS       DASRJB010000157         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2581675, whole genome shotgun sequence.
ACCESSION   DASRJB010000157 DASRJB010000000
VERSION     DASRJB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581675"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFB80_04435"
     CDS             <1..966
                     /locus_tag="VFB80_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN33038.1"
                     /translation="VPHWTIPIAICLGNCLILKTSEKVPLTAQRTAELLTEAGLPAGV
                     FSSIHGDGRVVEAICDHERIAAVTFVGSTPVARQVYARAAGKLKRVLALGGAKNHLVV
                     LDDADVPQTAVNVTASMSGCAGQRCMAAATLVAIGHVEQIIEQICHEARRIVPGQTLG
                     AVISRQSKERIERLISEAEAAGAKVLVDGRGAVVRGCENGFYIGPTVLDRVRPDMRIA
                     QEEVFGPVLAIIRARDIDEALAIETASPFGNAAAVYTRDGQAARHFVQRASSGMIGVN
                     VGVPVPREPFGFGGWNDSKYGVGDITGKSSIGFWTQAKKITTRWD"
     gene            972..2183
                     /locus_tag="VFB80_04440"
     CDS             972..2183
                     /locus_tag="VFB80_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN33039.1"
                     /translation="MNPGTIIFRGNVILPDGILAGGEVQVAGGRIIGVGQSTGAPATG
                     TVIDAGDGYVAPGFVDLHVHGGSGADFMDGTDEAFSTALAAHARHGTTSMAVTTTVAR
                     HDQILTVLETTRRFRERPAPRGARVLGAHFYGPYFRYEARGAHPGGPIRPPVAEEYLQ
                     YLEFADLLVTCTVAPEVLGAREFALACADRGIRTNVGHSWATFEQMAQAVQWGVRHVD
                     HLFCAMSDKTRLRQFQMYPMQGGVLEATLFFDELTTEVIADGKHLSADLLLLALKIKG
                     PDRLALVTDCNRALDMPDGNYMMGPLDGGEPMISRDGVGMMPSGTGLASSIQGMDHMV
                     RTFQALTGRPLWEVVRMASLTPARIAGWDCELGSIAAGKRADLVVLGSDMTVRRVFRD
                     GEEIYRSQSSA"
     gene            complement(2196..>4230)
                     /locus_tag="VFB80_04445"
     CDS             complement(2196..>4230)
                     /locus_tag="VFB80_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN33040.1"
                     /translation="DLALGPDGLIYAIHGDSVDVPSEQVTDFTSPFREARRGQRTSEG
                     HLIRTDRDGQKWEVLCGGLRNPFGVAFSPLGDAFTYDADAEFDMGSSWYRPTRIVQLA
                     AGADYGWRGVTGQWPPYFPDHADNALPVLDIGKGSPTAVAFGTETNFLSGEYQGALFV
                     LDWAYGRILAVHLALRGGGYRAQAETFLKGRPLNVTDLAIGPDKAMYIVTGGRKTQSA
                     LYRIAVSLNAFLEQPTSGHEQACQTHADKARVLRRMLESRHRGIGAEAVAFAWPHLDS
                     PDPVIRHAARIVVEHQPLETWRERALHEERTTAALTGLLALARSRDSSSLPAVLDRLG
                     AFSLEQLDVGQTLSLLDAYALCLEQTPQAMEERKQRIVAQLAPHYPHPAAQRHAACPL
                     GTGTHVQRKLARLLVHLEAPGAVEKTVRSLLESSVQEDRIHALLVLRSARSGWTIPLR
                     RAYFAALNEGATFVAGEGMPKFLTQLRDDASKTLSDAERKELADVLSVAAPPADETPP
                     PVRAVVKKWTLDDLAPMLSDASRKGDAAKGAGVFRDALCVRCHRVGARGPAVGPDLTH
                     VSGRFSRRDMLASILTPSKVVAENYRNVQVSTTDGRNILGRVLLEGDYRSEKLRIATD
                     PLRPSVIVELSKQEIEQYRETETSPMPDGLVDTFRAEEVLDLIAYLEGGARQP"
BASE COUNT          718 a         1398 c         1405 g          709 t
ORIGIN      
        1 gttccgcact ggacgattcc gatcgcgatt tgcctgggca actgcctgat tctgaagacc
       61 tcggagaaag tgccgctgac ggcgcagcgg acggcggagc tgctgaccga ggctgggctg
      121 ccggcgggcg tcttcagctc cattcacggc gacggccgcg tggtggaagc gatctgcgat
      181 cacgagcgga tcgcggccgt gacgttcgtc ggctcgacgc cggttgcccg ccaggtctac
      241 gcgcgggccg ccggcaagct caagcgcgtg ctggccctcg gaggcgccaa gaaccacctc
      301 gtcgtgctcg acgatgcgga cgtgccgcag acagccgtca acgtgaccgc gtcgatgtcg
      361 ggctgtgcgg ggcagcggtg catggccgct gcgacgctgg tggcgatcgg ccacgtcgag
      421 cagatcatcg agcagatctg ccacgaggcc cgccgcatcg ttcccggtca gacgttgggg
      481 gccgtgatct cgcggcagtc gaaggagcgc atcgagcggc tgatcagcga ggcggaggcg
      541 gcgggggcca aggtgctcgt ggacggccgc ggcgcggtcg tgcgcggctg tgaaaacggt
      601 ttctacattg gaccaacggt gctcgatcgc gtccgccccg acatgcggat tgctcaggaa
      661 gaagtgttcg gccccgtgct ggcgatcatt cgtgcccggg acatcgacga ggcgctggcg
      721 atcgagaccg cctccccctt cggcaatgcg gccgccgttt acacccgcga cggccaggcg
      781 gccaggcact tcgtccagcg ggcctcgagc ggcatgatcg gcgtgaacgt cggcgtgccg
      841 gtgccgcgcg agccgttcgg cttcggcggc tggaatgact caaagtacgg cgtcggcgac
      901 attaccggca agagttccat cggcttttgg acgcaggcca agaaaatcac gacgcggtgg
      961 gattaagcgc gatgaaccca ggcacaatca tctttcgcgg aaacgtcatc ttacccgacg
     1021 gcattctggc aggcggcgaa gtgcaagttg ccggcggccg gatcatcggc gtcggccaat
     1081 ctaccggcgc acctgcgacg ggcaccgtca tcgatgccgg cgatggttac gtcgcccccg
     1141 gctttgtcga tttgcacgtg cacggcgggt ccggggccga cttcatggac ggcaccgacg
     1201 aggcgttttc cacggcactg gcggctcatg cccgccacgg cacgacgagc atggcggtga
     1261 caacgaccgt cgcccgccac gatcagatcc tcaccgtcct ggaaacgacg cggcggtttc
     1321 gcgagcgtcc cgcgccgcgg ggggcccgcg tgctgggtgc acacttctac ggcccgtact
     1381 tccgctacga agcccgcggc gctcatccgg gcggcccgat ccgcccgccg gttgccgaag
     1441 aatacctcca gtacctcgaa ttcgccgatt tgctggtaac ctgcaccgtc gcaccggagg
     1501 tgctaggggc cagggagttc gccctggcct gcgctgaccg cggcattcgc accaacgtcg
     1561 gccactcctg ggccacgttc gagcagatgg cccaggctgt gcagtggggc gtgcggcacg
     1621 tcgatcactt gttctgcgcg atgtcggaca aaacgcggct gcggcaattc cagatgtatc
     1681 ccatgcaagg gggcgtgctg gaggcgacgc tctttttcga cgagctgacg accgaagtga
     1741 ttgccgacgg caagcacctg tcggccgatc tgctcttgct cgcgctgaag atcaaagggc
     1801 ccgatcggct agcgctggtg accgactgca accgggcact cgacatgccc gacgggaatt
     1861 acatgatggg gccgctcgac ggcggcgaac cgatgatcag ccgcgacggc gtggggatga
     1921 tgccgagcgg caccgggctg gcttccagca tccaggggat ggaccacatg gtgcgcactt
     1981 tccaggcgct gaccggccgg ccgctatggg aggtggtgcg gatggcgtcg ctgacgccgg
     2041 cgcggattgc cggctgggat tgcgagctgg gcagcattgc cgccggcaag cgggccgacc
     2101 ttgtggtact cggtagcgac atgacggtgc ggcgcgtgtt ccgcgatgga gaggaaatct
     2161 atcgcagcca atcctctgct tgataacaag caaggctacg gctggcgagc gcccccctcg
     2221 agatatgcga tcaaatcgag cacctcctcg gcgcgaaacg tgtccacgag accatcgggc
     2281 atcggcgagg tttccgtctc gcgatactgc tcaatttcct gcttggaaag ctcgacgatg
     2341 accgatggac gcaagggatc ggtggcgatg cggagctttt cggagcggta atctccttcc
     2401 aagagcaccc gccccaggat gttccgtccg tcggtcgtgc tgacctgcac gttgcgataa
     2461 ttctcggcga ctactttgga cggcgtgaga atcgatgcga gcatgtcccg gcggctgaag
     2521 cggccgctga cgtgcgttag gtccggaccg accgccggtc cccgcgcgcc aacgcgatgg
     2581 cagcggacgc acagcgcatc gcgaaacacg cccgcgcctt tcgcggcgtc ccctttgcgc
     2641 gaagcgtcgg aaagcatggg cgccaagtcg tccagcgtcc actttttgac caccgcgcgg
     2701 accggaggcg gcgtttcatc tgccggcggc gcggcgacag agagcacgtc cgccagctcc
     2761 ttccgctcgg catcgctgag ggtcttggag gcgtcgtcac gcaactgggt cagaaacttc
     2821 ggcattccct cgccggcgac gaaggttgca ccttcgttaa gcgcggcgaa ataggccctg
     2881 cgcagcggaa tggtccagcc ggagcgcgcg ctcctcagca cgagcagcgc gtgaatccgg
     2941 tcctcctgca cgctactttc cagcagcgag cggaccgtct tctcgaccgc cccgggcgct
     3001 tccaggtgca ccagcagccg cgcaagcttt cgctgcacgt gcgtccccgt ccccagcggg
     3061 caagcggcat gtcgctgggc cgccggatgc ggatagtgcg gtgcgagctg cgcgacgatc
     3121 cgctgcttgc gctcctccat cgcctgcggc gtttgctcca ggcatagcgc gtaagcatcg
     3181 agcagcgaca gcgtctgacc gacatcaagc tgttccagcg aaaacgcacc caatcgatcc
     3241 agcaccgccg gcagcgacga cgagtcgcgg ctgcgggcca gggccaacag tccggtcagt
     3301 gcagccgtcg tgcgctcctc gtgcagggcc cgttcccgcc atgtttccag cggctggtgc
     3361 tcgacgacaa ttcgcgccgc gtggcggata accgggtcag gcgaatcaag atgcggccag
     3421 gcaaacgcca ctgcctcggc gccgatgccg cggtggcgac tctccagcat ccgccgcagc
     3481 acgcgggcct tgtcggcatg ggtctgacat gcctgctcgt gtcccgaagt cggctgttct
     3541 agaaaagcgt tgaggctgac cgcgatgcgg tatagggccg actgcgtctt ccgtccgccg
     3601 gtgacgatgt acatcgcttt atctggacca atggccagat cggtcacatt cagcggccgc
     3661 cccttgagaa acgtctccgc ttgcgcccgg taaccgccgc cgcgcagcgc caggtgcacc
     3721 gccagaatgc gtccataggc ccagtcgagg acgaacagcg caccctgata ctcgcccgaa
     3781 agaaaattcg tctccgtccc aaaggcgact gccgtcggcg agcctttgcc gatgtcgagc
     3841 accggcagcg cattgtcggc atggtcggga aagtacggcg gccactgccc ggtcacgccc
     3901 cgccagccgt aatcggcgcc cgccgcaagc tgcacgatcc gcgtcggccg ataccagctc
     3961 gatcccatgt cgaattcggc gtcggcatcg taggtgaacg catctccaag cgggctgaag
     4021 gccacgccga atggattccg caacccgccg cacagcactt cccatttctg cccgtcgcgg
     4081 tcggtgcgga tcagatgccc ttcgctggtc cgctggccgc ggcgcgcctc gcgaaacggg
     4141 gacgtgaaat cggtgacctg ctcgctgggg acgtccaccg aatccccgtg gatcgcgtag
     4201 atcaaaccgt ccggccccaa ggccagatcg
//
LOCUS       DASRJB010000158         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_893578, whole genome shotgun sequence.
ACCESSION   DASRJB010000158 DASRJB010000000
VERSION     DASRJB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893578"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFB80_04450"
     CDS             <1..267
                     /locus_tag="VFB80_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33041.1"
                     /translation="SRDGKLFAGDGGDPGQVAFAVDGQWIYLYRPQADGTLAWERLVD
                     LSKHNYRLEPNVTITPDSRWVVFRSNMHGPTHVYAVEVAAASGR"
     gene            446..2941
                     /locus_tag="VFB80_04455"
     CDS             446..2941
                     /locus_tag="VFB80_04455"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN33042.1"
                     /translation="MPDRSTDRTLPLLLLLFVGSGCAALIYEIVWFQLLQLVIGSSAV
                     SLGVLLGTFMGGMCLGSLLLPRVIGRDRHPLAAYAYLELGIGAIGIAALFVVPLIGRL
                     YTAWAGYGIAGYFLRGAVAALCLLPPTVLMGATLPAIARWVEATPRGVSWLGFFYGGN
                     IAGAVLGCLLAGFYLLRIYDMATATYVAAAINLAVALVGLALAARTPYAAPARHREAA
                     TSAPVASANWTVLISIGLSGLAALAAEVVWTRLLSLMLGGSVYTFSIILSVFLIGLGI
                     GSSIASWLARGSVSPRVLLGGCQLLLAATIAWAAFQLARSLPYWPIDPSQASSPWHTF
                     QLDVLRCAWAILPSAVLWGASFPLALAAASGMRQLPDNHREANTSRSPDPGRLVGAVY
                     AANTIGAILGAVGTSVWLIPAAGTQYTQQLLILISAGSAVVALAPLLIGGETSSEASV
                     SRRPGLAGALALLAGLFAAWWLSRHVPPTPGELIAEGRYLATRHWEYPPLGSTQVQYQ
                     GEGMNSSVAVTLKGESTLSFHVSGKVEASTQPQDMRLQRMLGHIPALVHPNPRTVLVV
                     GCGAGVTAGSFLAHPEVERIVICELEPPIPKEIAPRFGRQNYDVVKDPKVQIVYDDAR
                     HYILTTRETFDIITSDPIHPWVKGAATLYTQEYFELCKARLNPGGVITQWVPLYESSM
                     DVVKSEVATFFAVFPEGTVWSNDSGGAGYDTVLLGHVGPLTIDVDRLEARVLSDDLLA
                     WSLEEVGFLSGLDLLATYAGTAQDLAPWTKDAQLNRDRNLRLQYLAGLSSHNYREAAI
                     YNAMVKHRTYPDKLFKASETNRVALMIKLGMQP"
     gene            2950..>3538
                     /locus_tag="VFB80_04460"
     CDS             2950..>3538
                     /locus_tag="VFB80_04460"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZN33043.1"
                     /translation="MLALTQTPSLRMDLGVRTYVGRTEIDFSRALDQHAEYCRLLAGC
                     GAAVRVLETNAHFPDGVFLEDTAVVLDEVAIVASMGTSARRGELPGIELVLREYREVE
                     RIELPALLEGGDVLRIDRKLLVGLSDRTDAAGIAALAALAGRYGYSLATAVARGCLHL
                     KTACTALPDGRLLVNPDWLDVAALKGSEWIAVPEQE"
BASE COUNT          512 a         1211 c         1180 g          635 t
ORIGIN      
        1 tcgcgtgacg ggaagctgtt tgccggtgac ggcggcgatc ccggccaggt cgccttcgcc
       61 gtggacggcc agtggattta tctctaccgg ccgcaggcgg acggcacgct cgcctgggag
      121 cggctggtcg acctgtccaa gcacaactac cgcctggaac cgaacgtcac catcactccc
      181 gacagccgct gggtcgtctt ccgctccaac atgcacgggc cgacgcacgt gtatgcggtg
      241 gaagtggcgg ccgcgagcgg ccggtgagtg gtgagtggtt ggtggtgagt ggtattactc
      301 gtttccaagc tccgcttggg aacgcaagct cccgaagctc tgcttcgtgt gcccggaagc
      361 ggagcttccg aacgtgggtt cccaagcgga gcttgggaac cagtccctga ccgaccgacc
      421 accgaccaac cgaccaccga ctcccatgcc cgaccgttcg accgaccgca cgctgccgct
      481 tctcttactc ctctttgtgg gcagcggctg cgcggcgctg atctacgaaa tcgtctggtt
      541 tcaactcttg cagctcgtga tcggctcgtc cgcggtgtcg ctgggagtac tgctcggcac
      601 attcatgggc ggaatgtgcc tggggagcct gttgctgccg cgggtgatcg gccgggaccg
      661 gcatccgctg gcggcgtacg cgtacctgga gctgggcatc ggcgcgatcg gcatcgcggc
      721 gctgtttgtc gtgccgctga tcggccggct ctacaccgct tgggccggtt acggcattgc
      781 cggctatttc ctccgcggcg cggtcgcggc gctctgcctc ttgccgccga cggtgctgat
      841 gggggccacg ctgccggcga ttgcccgctg ggtcgaagcc acgccgcggg gcgtttcctg
      901 gttggggttc ttttacggcg gcaacatcgc cggcgcggtc ctcggctgcc tgctcgccgg
      961 cttctatttg ctccgcattt acgacatggc gaccgccacg tacgtcgccg cggcgatcaa
     1021 cctcgcggtc gcgcttgtcg gcctggcgct ggcggcccgg acgccctatg ccgcaccggc
     1081 ccgccatcga gaggcggcga cttccgctcc ggttgcgagc gccaactgga ccgtcttgat
     1141 ttcgatcggc ctttccggcc tggcggccct cgccgcggag gtcgtctgga cccgcctcct
     1201 gtcgctgatg ctgggcggct cggtctatac gttttcgatc attctgtccg tgttcctgat
     1261 cggcctgggg atcggcagca gcatcgcctc gtggctggcc cgcggcagcg tttcgccgcg
     1321 cgtcttgctc ggcggctgcc agctcctgct tgccgcgacg atcgcctggg ccgcgttcca
     1381 gctcgcgcgg tcgctgcctt actggcccat cgatccctcg caggccagca gcccctggca
     1441 cacgttccag ctcgacgtgc tccgctgtgc ctgggcgatt ctgccctccg ccgtgctctg
     1501 gggggcgagc tttccgctgg ccctggccgc ggcgagcggg atgcgtcagc ttcccgataa
     1561 tcatcgggag gctaacacct cccgctcgcc cgaccccggc cggctggtcg gcgcggtcta
     1621 tgccgcgaac acgatcggcg cgatcctggg cgcagtcggc acgagcgtct ggctgattcc
     1681 cgcggccggc acgcaataca cccagcagct cctgatcctg atttcggcgg gctcggcagt
     1741 cgtcgcgctc gcgccgctgc tcatcggcgg ggaaacttcc agcgaagcca gcgtcagccg
     1801 gcgccccggc ctggccggag cgctggcctt actggccggc ctcttcgccg cctggtggct
     1861 cagccggcac gtgccgccga ctcccggcga attgatcgcc gagggccgct acctggccac
     1921 ccgccactgg gaatatccac cgctgggctc gacgcaggtc cagtaccagg gcgaagggat
     1981 gaactcgtcg gtcgcggtca cgctcaaggg cgaaagcacg ctcagcttcc atgtcagcgg
     2041 caaggtcgag gccagcacgc agccgcagga catgcggctg cagcggatgc tgggccacat
     2101 tccggcgctg gtgcatccga acccgcggac ggtgctggtc gtcggctgcg gggccggagt
     2161 gacggccggc tcgttcctgg cccatccgga ggtcgagcgg atcgtgatct gcgaactgga
     2221 gccgccgatc cccaaggaaa tcgccccgcg attcggccgg cagaactacg acgtggtgaa
     2281 ggaccccaag gtccagatcg tctacgacga cgcccgtcac tacatcctca cgacccgcga
     2341 gacgttcgac atcatcacct ccgacccgat ccatccctgg gtcaaaggtg cggcgaccct
     2401 ctatacgcag gaatacttcg agctctgcaa ggcccggctg aatccgggcg gggtgattac
     2461 gcagtgggtg ccgctgtacg aaagcagcat ggacgtggtc aagagcgaag tggcgacgtt
     2521 tttcgccgtc ttccccgaag ggacggtatg gagcaacgac tccggcgggg cggggtacga
     2581 taccgtgctg ctggggcacg ttgggccgct gacgatcgac gtcgatcggc tggaggcccg
     2641 cgtgctgagc gacgacctgc tggcctggtc gctggaggag gtcggcttcc tctcggggct
     2701 cgacctgctg gcgacctatg ccggcaccgc ccaggacctg gccccctgga ccaaagacgc
     2761 ccagcttaac cgcgaccgca acctgcggct gcaatacctg gcaggcctca gctcgcacaa
     2821 ctaccgcgaa gccgccatat acaatgccat ggtcaagcac cgcacctatc ccgataagct
     2881 gttcaaggcg tccgagacca accgcgtagc gctgatgatc aagctgggga tgcagccgta
     2941 gggtcagcca tgctcgcgct cacccaaacg ccgtcgctgc ggatggattt gggcgtccgg
     3001 acgtacgtcg gacggacgga gatcgacttc tcgcgggccc tcgaccagca cgccgagtat
     3061 tgccgtttgc tggccggctg cggagcggcg gtccgcgtgc tggagaccaa cgcccacttt
     3121 cccgacggtg tattcctgga agacacggcc gtcgtgctcg atgaagtcgc catcgtggcc
     3181 tcgatgggca catccgcccg ccgcggcgag ttgcccggga ttgaactcgt gctccgcgaa
     3241 tatcgcgagg tcgagcggat cgagctgccg gcgctgctgg aagggggcga cgtgctgagg
     3301 atcgaccgca agctgctggt tggcctttcg gaccggaccg acgccgcggg aattgccgcc
     3361 ctggccgccc tggccggccg ttacggctac tcgctggcga ccgccgtcgc ccgcggctgc
     3421 ctccatctga agaccgcctg cacggcgctg cccgacggcc gcttgctggt gaatccggat
     3481 tggctcgatg tggcggccct caaaggcagc gagtggatcg ccgtgcctga gcaggagc
//
LOCUS       DASRJB010000159         5637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2300726, whole genome shotgun sequence.
ACCESSION   DASRJB010000159 DASRJB010000000
VERSION     DASRJB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300726"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFB80_04465"
     CDS             complement(<1..329)
                     /locus_tag="VFB80_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33044.1"
                     /translation="MPDIVLENRRVLSTWRVPLSRTYVARFGAPVVTHVDPLIFLRKF
                     FAYCMDCDFCHDSCCRFGCDTSLATVENLETKFGNDLEQYTGTSRADWFEPEVYADRD
                     FPGGRFRS"
     gene            complement(395..1456)
                     /gene="aroF"
                     /locus_tag="VFB80_04470"
     CDS             complement(395..1456)
                     /gene="aroF"
                     /locus_tag="VFB80_04470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZN33045.1"
                     /translation="MNALLPTFTGIRELFSMIVVMEQGATAEQIRHMAERVESLGLKA
                     HVIQGTERTVIAAIGEKRDGTRESLASGPGVESVMPILAPYKVASREVKKEPTQVAVR
                     SLRVGAGHVGIIAGPCSVESEEQILASARAVKAAGGTALRGGAFKPRTSPYSFQGLKE
                     EGLKLLALARQETGLAIVTEVMSTEEVELVAQYADVLQVGARNMQNYRLLESVGQARK
                     PVLLKRGPSATIEELLLAAEYILNEGNPEVMLCERGIRTFEAHTRFTLPLATVPYLHH
                     KTHLPIVVDPSHGTGHTYLVPDMAVASIAAGADGLILEVHPDPERALSDGYQSLNFPQ
                     FLDTIARCRAVAAALGKTL"
     gene            complement(1776..3185)
                     /locus_tag="VFB80_04475"
     CDS             complement(1776..3185)
                     /locus_tag="VFB80_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33046.1"
                     /translation="MTRTASRRQFLQTSAAAAAAASVPYFGWSAQTIAQDAKTESKND
                     RPLLGCIGTGDRWNGVGPQAMNHADCVAVCDVDANHMEKGKQRALDSNSKKGRERTVE
                     AYEDYRKLLDRKDIEVVTIVTPDHWHSKIAIEAMKAGKDVYCEKPLTLTIDEGKQILK
                     VLKETGRVFQVGTQQRSEMQAERGKFPQQFLTAVALCREGRLGKIQKVECRIGGSTKS
                     PELPKVPVPTGLNWEMWLGQAPLVDYVSSPTGSSNRGYPASRCHYEFRWWYEYSGGKM
                     TDWGAHHVDIATWAIGMENSGPTSVEGVMAVHPVDFKDGWPTADNQYNAATKFDVKCL
                     YPNGVELHILSDGENGIQITGDKAELFVSRGNLRGTAVDALKDMPLPDETIAKLYKGK
                     KPGNHMANFFECCQDRTQPISDVPTHHRAMTTCHLANIAIRLGRKLTWNPETEQIVGD
                     EAAAAFCKREQRKGYEIVT"
     gene            complement(3304..3762)
                     /locus_tag="VFB80_04480"
     CDS             complement(3304..3762)
                     /locus_tag="VFB80_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33047.1"
                     /translation="MKLIELMPLLARGEEGFFVVAIVVALGIALAIRLLAGTLDHDRI
                     RQYVQQRGGSVRSIQWSPFGHGWFGDKHHRIYEVAYDDADGNPHVATCKTSLWSGVYW
                     TEDQVAGRQPFARLPRPPANVFEDPAQLAAENQRLHEEVQRLKQELKHRG"
     gene            complement(3815..>5637)
                     /locus_tag="VFB80_04485"
     CDS             complement(3815..>5637)
                     /locus_tag="VFB80_04485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN33048.1"
                     /translation="KAKAVTADKHRGIVSYFWAYTNKHIVYTQDKNGDEDDHVYCVDL
                     ESGQTRDLTPIAKIAAEIDTVSEKFPSEIVVGINDRGERQFHDLYRINILTGERKLLQ
                     ENPGMAGFLCDDDYRVRFALNITEQGGQELLKPAAGGKWEPMIKIDALDAMTTSPAGF
                     DKTGEHLFFLDSRNRDTTALTTIDLKSGEQKLLAADPRADLSGALTHPTEKNVQAVSF
                     TYARTEWKILDESIRGDLDYLKTVADGEVQITGRTLDDKRWTVAYLMDNGPVRFYIYD
                     RQPAKKTTFLFTSDREQEKLPLVKMHPQTIPSRDGLQLVSYLTLPKWTDPDGDGRPDR
                     PLPLVLNVHGGPWARDDWGYDPEHQLLANRGYAVLAVNYRGSTGFGKDFINKANREWA
                     GKMHDDLIDAVNWAVAEKIAAKDKVAIMGGSYGGYATLVGLTFTPEVFACGVDIVGPS
                     SLITLAENPPPYWFSFLPIMKLRVGDWTTPEGRKFLESRSPLFKANKIVRPLLIGQGA
                     NDPRVKQSEADQIVKAMNDKKIPVAYALFHDEGHGFARPENRFAFYAITEAFLAKHLG
                     GRAEPIEGAFKGADFSMPSGKDEVPGAAEALAKQKERVEK"
BASE COUNT          875 a         1791 c         1848 g         1123 t
ORIGIN      
        1 gagcgaaacc gtccgccggg gaagtcgcgg tcggcgtaga cttccggctc gaaccagtcg
       61 gcccgcgacg tgcccgtgta ttgctccagg tcgttgccga atttcgtttc gagattctcg
      121 accgtcgcca ggctcgtgtc gcagccgaag cggcaacagg agtcgtggca gaagtcgcag
      181 tccatgcagt acgcgaagaa cttgcgcagg aagatcagcg gatcgacgtg ggtcaccacc
      241 ggcgcgccga accgcgcgac gtaggtccgt gagagcggca cccgccacgt gctgaggacg
      301 cggcggtttt ccaggacgat atcgggcatc cggccactcc ggttggcggc gctagtacat
      361 tcggcagacc ggaatgtctg ccccatgtgc acgtttatag cgtcttgccc agcgcggccg
      421 ccacggcccg gcagcgggca atcgtatcga gaaattgcgg aaaattgagc gactgatagc
      481 cgtcggagag ggctcgttcg ggatcgggat ggacctccag gatcaagccg tcggcgccgg
      541 cggcgatcga ggcgacggcc atatcgggca cgagatacgt gtggccggtg ccgtggctgg
      601 gatcgacgac gatcggcagg tgcgtcttgt gatgcaggta gggcaccgtc gccagcggca
      661 gcgtgaatcg cgtgtgggct tcgaaggtgc ggatgccgcg ctcgcagagc atcacctcgg
      721 gattgccttc gttgaggatg tactcggcgg ccaggagcag ctcttcgatc gtcgcgctgg
      781 ggccgcgctt gagcagcaca ggcttccgcg cctgcccgac cgactccagc aggcgatagt
      841 tctgcatatt ccgcgcgccg acctgcagca cgtcggcgta ctgggcgacg agctcgacct
      901 cttcggtgct catcacttcg gtcacgatcg ccaggccggt ttcctggcgg gcgagggcca
      961 gcagcttgag gccctcctct ttgaggccct ggaagctgta ggggctggtc cgcggcttga
     1021 acgctccgcc gcggagggcg gtgccgccgg cggccttgac cgcgcgggcc gaggcgagaa
     1081 tctgctcttc gctttcgacc gagcaggggc cggcgatgat gccgacgtgc cccgcaccga
     1141 cccgcaggct ccgcacggcg acctgcgtcg gctctttctt cacctcgcgg ctggcgacct
     1201 tgtacggcgc gaggatcggc atcacgcttt ccacgcccgg gccgctggcc agcgactcgc
     1261 gcgtgccgtc ccgcttttcg ccgatggcgg cgatgacggt ccgctcggtg ccttgaatga
     1321 cgtgggcctt caggcccagg ctctcgaccc gctcggccat gtgccggatc tgctcggccg
     1381 tggccccctg ctccatgacg acgatcatcg aaaacaactc ccggattccg gtaaatgtgg
     1441 gtagtagggc gttcatggcg ggtggtcagt ggtcagcggt cagttgcaga tcgggcttcc
     1501 aagcaccact gacaactgac taccgacaaa gccccaaccg aaaaacgccc gccaggttca
     1561 acaaaaaacc ccgagcgacc ttccggtcct cggggctgta gttggctgaa atgcggcggt
     1621 tcttagcgac gcacctcccg cggccccggc ggcccgaagg taaagctaaa ccaccaaaag
     1681 taaccgcggc tgcgtcgcat gggacgtatt atggtccgaa aatgcagtgt tgcaagtagt
     1741 ggttggtcag tcgttagttc cctactggcc actgactaag tgacgatctc ataccccttc
     1801 cgctgctcgc gcttgcagaa ggcggcggcg gcttcgtcgc ccacgatctg ctcggtctcg
     1861 gggttccagg tgagcttgcg gcccaggcga atcgcgatgt tcgccaggtg gcaggtcgtc
     1921 atcgcccggt ggtgcgtagg cacgtcgctg atcggctgcg tgcggtcctg gcagcactcg
     1981 aagaaattcg ccatgtggtt gccgggcttc ttgcccttgt agagcttggc gatcgtctcg
     2041 tcgggcagcg gcatgtcttt tagcgcatca acggccgtgc cgcggaggtt gccgcggctg
     2101 acaaacagct cggccttgtc gccggtgatc tgaatgccgt tctcgccgtc cgacagaatg
     2161 tgcagctcga ccccgttggg atagaggcac ttcacgtcga acttcgtggc cgcgttgtat
     2221 tgattgtccg cggtcggcca gccgtccttg aaatcgacgg gatgcacggc catgacccct
     2281 tcgacgctgg tcgggccgct gttctccatg ccgatcgccc aggtggcgat atcgacgtgg
     2341 tgcgcgcccc agtcggtcat cttgccgccc gagtattcgt accaccagcg gaattcgtag
     2401 tggcagcggc tggcggggta gccgcggttc gagctgcccg tcggcgatga gacgtaatcg
     2461 accagcgggg cctgccccag ccacatctcc cagttgaggc ccgtcggcac cggcaccttc
     2521 ggcagctcgg ggctcttggt cgagccgccg atccggcact cgaccttttg aatcttgccc
     2581 agccgtccct cgcgacagag ggccacggcg gtcaggaact gctgcgggaa cttgccccgc
     2641 tcggcctgca tctcgctccg ctgctgcgtg ccgacctgga acacgcggcc ggtttctttc
     2701 agcaccttga gaatctgctt gccttcgtcg atcgtcaggg tcagcggctt ctcgcaatag
     2761 acgtccttgc cggccttcat cgcttcgatc gcgatcttgc tgtgccagtg gtcgggcgtg
     2821 acgatcgtca ccacctcgat gtccttgcgg tcgagcagct tgcgatagtc ttcgtacgct
     2881 tcgacggtcc gctcgcggcc cttcttgctg ttggaatcga gggcccgctg cttgcccttc
     2941 tccatgtgat tcgcatcgac gtcgcacacc gccacacagt cggcatggtt catcgcctgc
     3001 ggcccgacgc cattccagcg gtcgcccgtg ccaatgcacc cgaggagcgg ccgatcgttc
     3061 ttgctctcgg tcttggcgtc ctgggcgatg gtctgtgccg accagccgaa ataggggacg
     3121 ctggcggcgg cagccgcggc ggcggaggtc tgcaggaact gacggcggga agcggtgcgt
     3181 gtcatgggag ggtctttcgt gcaaaaaagc gacaagaaga gccggattgg cgcggagaac
     3241 ggctgcccaa ggcgctggtc agtgtagcgg ggcggcaacg cgatttccag ctttcgagtt
     3301 gttctacccg cggtgcttta gctcctgctt caggcgctgc acttcctcgt gcaaccgctg
     3361 attctcggcg gcgagctgcg cggggtcttc gaagacgttg gcagggggcc gcggaagcct
     3421 ggcaaacggc tgccggccgg cgacctggtc ctcggtccaa tagacgccgg accagagcga
     3481 cgtcttgcag gtggcgacgt gcgggttgcc gtcggcgtcg tcgtaggcga cttcgtatat
     3541 ccgatggtgc ttgtcaccga accagccgtg gccgaagggc gaccactgaa tgctccgcac
     3601 cgagccgcct cgctgctgga cgtattggcg aatccggtcg tggtcgagcg ttcctgccaa
     3661 gaggcggatc gccagggcga tgcccagcgc gacgacaatc gctacgacga aaaagccttc
     3721 ctcgccgcgg gcgagcagcg gcatcagttc aatgagtttc ataatcgcct ttgcgacttc
     3781 tgcagctttg caactttgcg ttaaagcccg agtcctattt ttcgacccgc tccttctgtt
     3841 tcgcaagcgc ctccgccgct cccggcactt cgtccttccc gctgggcatg ctgaagtcgg
     3901 cgcccttaaa ggcgccctcg atcggctcgg cgcggccgcc gaggtgtttg gccaggaagg
     3961 cctcggtgat ggcgtagaac gcaaagcggt tctcgggtcg ggcaaagccg tgcccttcgt
     4021 cgtggaacag ggcgtaagcc accggaatct tcttgtcgtt catcgccttg acgatctggt
     4081 ccgcctcgga ctgcttgacc cgcgggtcgt tcgctccctg gccgatgagc agcggccgca
     4141 cgatcttgtt ggccttgaac aggggcgatc gcgattcgag aaacttgcgg ccctcgggcg
     4201 tcgtccagtc gccgacgcgg agcttcatga tcggcagaaa gctgaaccaa tagggcggcg
     4261 ggttctcggc cagcgtgatc aggctcgacg gcccgacgat atcgacgccg caggcaaaga
     4321 cctcgggcgt gaacgtcagg ccgaccagcg tcgcatagcc gccgtagctg ccgcccatga
     4381 tcgccacctt gtccttcgcg gcgatctttt cggccaccgc ccagttgacc gcgtcgatca
     4441 ggtcgtcgtg catcttgccg gcccactcgc ggttggcctt gttgatgaag tccttgccga
     4501 agccggtcga gccccggtag ttgaccgcca gcaccgcgta gccgcgattg gccagcagct
     4561 gatgctcggg atcgtagccc caatcgtcgc gagcccacgg gccgccgtgc acattcagca
     4621 ccagcggcag cggccgatcg ggccggccgt cgccgtccgg atcggtccac ttgggcagcg
     4681 tcagatagct gacgagctgg aggccgtcgc gggacggaat cgtctgcggg tgcatcttca
     4741 ccagcggcag cttctcctgc tcgcggtcgc tggtgaacag gaatgtggtc ttcttggccg
     4801 gctgccggtc gtagatgtag aaccgcaccg ggccgttatc catcaggtag gccacggtcc
     4861 accgcttgtc gtcgagcgtg cggccggtga tttgcacctc cccgtcggcg acggttttga
     4921 gatagtccag gtcgccgcgg atcgattcgt cgaggatctt ccactcggtg cgggcgtagg
     4981 tgaagctcac cgcctgcacg ttcttctcgg tcggatgcgt cagggccccg ctgagatccg
     5041 cccgcggatc ggcggccagc agcttctgct cgcccgactt caggtcgatc gtcgtcaggg
     5101 cagtcgtgtc gcggttgcgg ctgtcgagaa agaacaggtg ctcgcccgtt ttgtcgaagc
     5161 cggcggggct ggtggtcatg gcgtcgaggg cgtcgatttt gatcatcggc tcccacttgc
     5221 cgccggcggc cggcttgagc agctcctggc ccccctgctc ggtgatgttc agggcgaacc
     5281 gcacgcggta gtcgtcgtcg caaaggaacc cggccatgcc gggattttcc tgcaagagct
     5341 ttcgctcgcc ggtgaggatg ttgatccggt acaggtcgtg aaactgccgc tcgccgcgat
     5401 cgttgatgcc cacgacgatc tcgctgggaa atttctcgct gaccgtgtcg atttccgcgg
     5461 cgatcttggc gatcggcgtc aggtccctgg tctggcccga ttccaggtcc acgcaataga
     5521 cgtgatcatc ttcgtcgccg ttcttgtcct gcgtgtagac gatgtgcttg ttcgtgtagg
     5581 cccagaagta gctgacgatg ccgcggtgct tgtcggcggt caccgccttg gccttcg
//
LOCUS       DASRJB010000160        17570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3994127, whole genome shotgun sequence.
ACCESSION   DASRJB010000160 DASRJB010000000
VERSION     DASRJB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994127"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1102)
                     /locus_tag="VFB80_04490"
     CDS             complement(50..1102)
                     /locus_tag="VFB80_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN33049.1"
                     /translation="MPPDDDIQAVHRLKEAYQQITTELAKVVIGQKQVIEELLVAMFA
                     RGHCLLVGVPGLAKTLMIRTVADALALQFSRIQFTPDLMPSDITGTEVIQEDKATGHR
                     EFRFVSGPIFGNVILADEINRTPPKTQAALLEAMQEHQVTVGGRKHLLPEPFFVLATQ
                     NPIEQEGTYPLPEAQLDRFMFHVWVDYPSAEEELEIIKRTTADIEVKITPTLHAEDIA
                     ALTKIVRKVPVADHVARYAMQLARLTRRQEPDAPEFVREYVQWGAGPRASQYLVLGAK
                     ARAVLLGRFFVTHDDIRAIAPPVLRHRLKMNFNADAAGVSADEVIRRLLAHVPFAADE
                     APARGIPDVYRAANAG"
     gene            complement(1146..3545)
                     /locus_tag="VFB80_04495"
     CDS             complement(1146..3545)
                     /locus_tag="VFB80_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33050.1"
                     /translation="MRQIQQLTLDHRPPPTALLVLALVLLLARATQAQILERSPALPP
                     AEVDLLDAASAAHLENAQRFLAEKQWAEAVEAIRRVQETDASRLVKVGLMRPVGGFER
                     YVPASEYCQWRLASLAATAPEALAHYRRLVDSLAEGWLQQGAKNNDEALLRRVVQQAF
                     ASRAADDALLKLGDLALSRGEPALAREFWERLGPNLTVPPQAAAALRTPAGSPLWLPL
                     RNFDFAGRGPELAALLQVADPPAAGIYPDTDLDLAAVRARLVLASLLEGSRRRAEIEL
                     AILRAVSPTAEGTLAGRQGRYADLLGSLLEESASWPPPRQSTDWPTFGGNEARSKTVA
                     ADLDPAGKPLWTYQLPRLTSDRDWLGTGRLRVGDDMKSLLSYHPVVVGQKVYVRLDAR
                     GNSYVAALDLKTGQPVWQVDYRRGLSPQRDDAAGPWEVSDAHAGLVRHIGVARYTLSA
                     GGGKLFARMGSPLTAPRERMNQPLLAKEQGFLLGLDLATQGKPLEGFPIRPESLDWSF
                     EGTPLAAGGTIYVAMRKLEGARTQLHLAAFELQTTSLGVVDDRAEDARPAGRMRWRTR
                     ICSALSPGGGDFDCLSHLLVTLDSGRLYLNTNCGAVAAVDAADGRLLWLVKYPRAEIR
                     LNDPDRSSAHLFRDVTPCLAWKDLIIVAPSDCDRLFALYASSGQLAWTSEVDAVHLLG
                     VEGDVLLASGDALYWIDVNTGRQLAQFPPGKLGGAEQAAASPRGLGRGVVAGGHVWFP
                     TRESIFVFETQPAAGDFGQQPRLVRTIPLFPRGVTGGNLVIAEGILLIAGGDKLTAFA
                     Q"
     gene            complement(3566..3775)
                     /locus_tag="VFB80_04500"
     CDS             complement(3566..3775)
                     /locus_tag="VFB80_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33051.1"
                     /translation="MVYSFGRLLQAAGLAILPLAMVMQLTGGLRAPTGNVSVSVMLLL
                     MVFGVVLFSLGRVVEGYAGKKLPGE"
     gene            complement(3830..4981)
                     /locus_tag="VFB80_04505"
     CDS             complement(3830..4981)
                     /locus_tag="VFB80_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33052.1"
                     /translation="MTDTIRRISWRDLFPWLILLRTFRIAISPPLLALATAAVLIAPL
                     GWRLAGLVFQPKFESGTTNLPVYQAAGENSFIAQWTPPAVREYLPGAQTAFLEAYQQL
                     AEPLARLFRMRITLREMAYYAFGFLWTLALWSFPGAVISRHAVVNLAAESPPGIVETA
                     RFAARRWHWYVLAPLYPLVPVLIVALGIAILGLPIQFAPGAGSILAVLAWIFVAIASL
                     GVLWLFGGLLFGFPLMWPTISAERDGDAFEAFSRSYSYVYGKPLHYFFYVVVAALFGA
                     LCWAVVSGAATLLQEFGFWALAWGGTAAKVEPLRFAALQFALGLPAPPNQGWAFSLGT
                     VLIGLVLCVIHGVVAGFRFTYFFTVASAIYLLLRQDVDEKEMDEVFIST"
     gene            complement(5076..5264)
                     /gene="yacG"
                     /locus_tag="VFB80_04510"
     CDS             complement(5076..5264)
                     /gene="yacG"
                     /locus_tag="VFB80_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZN33053.1"
                     /translation="MSLLRCPICDRQFDPAASQAMPFCSDRCRQIDLGRWLREAYSVP
                     VERDPDEEAEESPPQDER"
     gene            complement(5279..11554)
                     /locus_tag="VFB80_04515"
     CDS             complement(5279..11554)
                     /locus_tag="VFB80_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZN33054.1"
                     /translation="MRSTFILRLAAAGLFLVGLVAVASSQNPTPAELRTSSQTLMRNG
                     NFKEAYDGFRRLCLNPNAGAAQVSQDLTNAVQCLNRLGRVKEFDELVESTVKTHQGNW
                     RLLQTAAQLYQQTQHQGFRIAGQYERGPHRGGGEMINSVERDRVRALQLMTQAMPLAQ
                     KDDRKDEVSQFFLNYGELLLNNRGYYEAWRLQYLTDLTTLPDFDEGYFYYRDYNGAPV
                     DAEGKPNYHIVPASWEASKTDGQRWRWVLSQAIENSPTRLNAVRYHLAQFFEQQFGVQ
                     SMAQGRRGFGRGFFAPQSDDDTKKDESGTYALHTLKENETIAKLASGIKRFELPDEFN
                     HIKLYQQIIAEPRTGLEEQSLQQLAEVFENRRQYPRAAEYWKTSIAKFGPGQNNWKQL
                     RSEQIVGNWGTFEATNSQPAGQGATVDFRFRNGKKVKFDARAIKVDLLLEDLKAYLKS
                     DPANRLDWNKINLGNIGYRLVQQNETKYLGEAAAAWELDLEPRPNHFDRRITVSTPLQ
                     KAGAYLLTGSMEGGNVSKIVLWVADTAIVHKQLSGKNLYFVADAAGGAPIPEINVEFF
                     GWQQRSLGGNRYQVVTTNFAELTGPDGLLIPNPIDLKPEFQWLVVARGKGATPRLAFL
                     GFMGVWTGQYYDPEYNQVKIFTMTDRPVYRPNQKVQFKLWVERAQYDNDKSDFAGTTL
                     PIAIFNPKGEKILSKTLMADAFGGMEGEYDLPADAVLGQYMIQLDEGHNLPLQAIEGN
                     HFRVEEYKKPEFEVTIKAPTEPVMLGEKITAKIEAKYYFGSPVTKATVKYKVLRTDYS
                     QDWYPLARWDWCYGPGYWWFAYDYPWYKGFRDWAGCLRPTPIWWPRGNVTPPEVVAEV
                     EREIGPEGTIEVEFDTQVAKELHGNTDHRYTITAEVRDESRRTIVGEGKVLVARKPFK
                     VFSWVDRGYYRVGDTIAASFSAQTLDQKPVKGEGVLKLLKISYDDKQQPIETPLQQWP
                     LATDAEGRAAQQLKASAKGQYRLSYSLTDQQKHTIEGGYIFTIIGDGFDGSEYRFNNV
                     ELMPDKAEYSPGESVKLQLNTNRADATVLLFLRPTNGIYLEPKVLRMKGKSSLQEIAV
                     IKKDMPNFFVEAVTVFGGDVHSETKEIVVPPEKRVLNVAVLPSKEQYKPGEKGRVKIH
                     LTDVNGENFVGSTVLTMYDKSVEYISGGSNVGDIKKFFWEWRRHHHPSHQDSLAKWSY
                     NLALPGKQSMAFIGVFGASVADDLDAGDNMQLAKADGNANGLANRGGGLGGFGGGGRQ
                     ALLRGMAAEGAPMAAAPPGAPLGEDKAADRANFAAEGLFSEQRKRAGEAEFQQGQQQG
                     GPLVEAAVRTKFADTAKWVGSLTTDKTGVGEIEVDMPENLTAWKIRVWAMGHGTKVGS
                     GEADVVTRKNLIIRLQAPRFFVQKDEVVLTANVHNYLAAEKNVSVMLEVPGGVLQPLD
                     GVNSTQVVKVPANGEARVDWRCKVLNEGEAIVRMKALTDEESDAMEMKLPSYIHGMLK
                     TESWAGTIRPDKDSSKLAISVPAERRVDQSQLEIRYSPSLALAMVDALPYLAEYPYGC
                     TEQTLNRFLPSVITQKTLMRMNLNLAEIRDKRTNLNAQEIGDDRERAKQWKRFDHNPV
                     FDEAELSRMVKEGVKALTEQQISDGGWGWFSGVGERSWPHTTAVVVHGLQIAKANDVA
                     LVPGVVEKGVEWLQRYQAEQLQWLKNFEQKLENVPKKQYADALDAMVYMVLVDAASDN
                     ALMRDRLYRDRIELPVYAKAMFGVALDKVGDKEKLAMIVQNVSQFLVEDPENESAYLK
                     LPENNWWWYWYGSEVEANAYYLKLLAKVDPKGEKAPRLVKYLLNNRKHGTYWNSTRDT
                     ATCVEAFADYLQAAGETEPDMFVEVWLDGAKLKEVQITKENLFTYDNKFVLSGAEVKN
                     GAHEIELRRRGKGPVYFNTYLTNFTLEDHITKAGLEVKVERQYYKLVPVDKTVKVEGT
                     RGQPLDQKVEKYERQLLKEGDTLKSGDLVEVELVIESKNDYEYLLFEDMKAAGFEAVE
                     QRSGYGTKGLPAYVEYRDNRVTFFVRWLARGKHSVNYRLRAEVPGKFAALPTRASAMY
                     APELKGNSDEIKLNIVD"
     gene            12867..14015
                     /locus_tag="VFB80_04520"
     CDS             12867..14015
                     /locus_tag="VFB80_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33055.1"
                     /translation="MGGAASPLKVASPAQPSSTVLALERKRHHPILISAACIGVVLIT
                     AVVFVVVLANQRRDATLAQVAPPAPAPEGPKPEPRLGESAKTGQPKPPPEVEQPVARP
                     NPTPMPPEPPPRPMPESPPTPVATPTPAPMPSPPPMPAPNPTPPVTPQPMPTPAPMPT
                     PPPMPAPPPPPPAPMATKAEITALIKALAAAKGALADQDFAEADQQLARAENLAKLPK
                     HQDAVARLKLVGAYVKQFRDAVATAVSNFEAGSVFMVGSSTQVAFVEGFPDRVTLRIA
                     GMNKTYPFRDMPLGLALAIADQRLLESDPVNRVVKGSYVLVHKRADSESHEKAKTWWT
                     EAQTGGVDMSPLMPFLTDNYAEFLKDASDAKPAASGDAKPAASPPAAQ"
     gene            14114..16276
                     /locus_tag="VFB80_04525"
     CDS             14114..16276
                     /locus_tag="VFB80_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZN33056.1"
                     /translation="MHSLASTRLSRGLSLGFGFVRRNLWLWPLLAAAMLFVAGHWIRN
                     SVESAAQMEMAEGLNATLTSNVEALKIWLDSEESRAREAALDPGVRRAAGELARLASS
                     SGDDPIALAQSPLQEELKAALRTFTSGQRYDGYVLLNKDQLVLASNRPELIGQVRTTN
                     YDLTARTLDKGALVTPPFPSVLLSTGPDGVARAGQPTMFALARQLDLTGDPIGVVGLR
                     IRPDRDFVRILGIARPGSSGETYAFDKDGLMLSESRFTPDLIRMGLVPDQPGARSQLT
                     LSIRDPGVDQTTGARPAKTRAEQPLTVAAASAVLGQSGYNVQGYRDYRGVPVVGAWTW
                     LGEYGIGVVTEKDVAEAYHSLYLLRRAFWSLFGLLAAAAGLIFVFTLAMDRLNRAAQN
                     AALAAKELGQYQLEEKLGEGGMGVVYRGRHRMLRRPTAIKLLHPDKTTDEAIARFERE
                     VQLTCQLNHPNTIAIYDFGRTPEGIFYYAMEFLDGITLDTLVARFGPQPAGRVVHILR
                     QVCGSLAEAHEIGLVHRDIKPANIILSCRGGEADVAKVLDFGLVKAVDSKKNASITSA
                     GAVLGTPLFMSPESIQSPETIDVRSDLYALAAVGYFLVTGQPPFSGASVVEICVHHAR
                     TPPMRPSEKLGLPVPADLEDVLLKGLAKKPEDRFSTAREFAEALAACADAAAWKSSTA
                     DAWWRTNYPGNAAATISIDQTNSIHGEQTLITTAPALK"
     gene            complement(16290..17219)
                     /locus_tag="VFB80_04530"
     CDS             complement(16290..17219)
                     /locus_tag="VFB80_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33057.1"
                     /translation="MFTSIRRHAAAVAILLTAACPLAAEEWPADTIDLSAVTVEQPAN
                     ASIWLLGGQMPAGEEAPLVPIGGEHPPYPTFPPCWCKEWKPWYVGVEGGWTIPLNVTP
                     DSNGVGAFFDGVAFGTQLHGGENFTLRLGLRLSPRWRADVSYTNIHGDYQWRTAFFGG
                     AQIAGFDSDATSNIVLGNAYWHFFPPAEHCNRPRFDPYTGVGLGVAFNTLAATAEFPD
                     GNPAGQYATVFGDTTACLATRFALGTHFWIWPNLAMDMSFAVLYVGDFQTDDFRTTGG
                     IVQPIGRYDFHNNWLGTFQLGFVWFPQPGFYRR"
BASE COUNT         3082 a         5793 c         5511 g         3184 t
ORIGIN      
        1 gacgtagccc tccacgatgt ggcgagcccg caggcggagg cccttgagct tagccagcgt
       61 ttgcggctcg atagacgtcg gggatgccac gtgcgggagc ctcgtcggcg gcgaaaggaa
      121 cgtgtgccag caggcggcgg atcacctcgt ccgccgaaac gcccgccgca tcggcgttga
      181 aattcatctt cagccgatgc cgcagcaccg gcggggcgat cgcccggatg tcgtcgtggg
      241 tcacgaagaa ccggcccagc agcaccgcgc gggccttcgc gccgagcacg aggtactggc
      301 tggcacgcgg gcctgcgccc cactgcacgt attcgcggac gaactccggc gcgtcgggct
      361 cttgccgccg tgtcaggcgg gcgagctgca tcgcgtagcg ggcgacgtga tcggccaccg
      421 gcaccttgcg gacgattttg gtcagtgcgg cgatgtcttc ggcatgcagc gtcggcgtga
      481 tcttgacctc gatgtcggcc gtcgtccgct tgatgatctc cagttcttcc tcggccgacg
      541 ggtaatcgac ccagacgtgg aacatgaagc ggtcgagctg ggcctcgggc agcgggtacg
      601 ttccttcctg ctcgatcggg ttctgcgtcg ccagcacgaa gaacggctcc ggcagcagat
      661 gcttgcggcc gccgacggtg acctggtgct cttgcatcgc ctcgagcagc gcggcctgcg
      721 tcttgggcgg cgtgcggttg atctcgtcgg ccaggatcac gttgccgaag atcggcccgc
      781 tcacgaagcg aaactcgcgg tggcccgtgg ccttgtcttc ctgaatgacc tccgtccccg
      841 tgatgtcgga gggcatcaag tccggcgtaa actgaatccg gctgaactgc agcgccagcg
      901 cgtcggccac ggtgcggatc atgagagtct tggccaggcc gggtacgccc acgagcaggc
      961 agtggccgcg agcgaacatc gccaccagca gctcttcaat cacctgcttt tggccgatca
     1021 cgaccttcgc cagctccgta gtgatctgct gataggcctc tttgaggcgg tgaacggctt
     1081 gaatgtcgtc gtcgggcggc atggcggaag gtagatcggt ggtcagtggt cggtaagtcg
     1141 gtgggctact gggcgaacgc ggttagttta tcgccgcctg ctattaatag tattccctcg
     1201 gcgatcacca ggttgccgcc ggtgacgccg cgggggaaga gcggaatcgt gcggaccagt
     1261 cgcggctgct ggccgaaatc gccggccgca ggctgagtct caaagacgaa aatgctctcc
     1321 cgcgtcggga accagacgtg gccgcccgcg accacgcctc ggcccagtcc gcgcggcgaa
     1381 gcagcggctt gctccgcgcc gccgagcttt cccggtggaa actgggcgag ctggcggccg
     1441 gtgttgacgt cgatccaata cagcgcgtcg ccgctggcca gcagcacgtc gccctccacg
     1501 cccagcaggt gcacggcgtc aacctcggag gtccaggcga gctgtccgga ggaagcgtac
     1561 aaagcaaaca gtcgatcgca gtcgctcggc gcgacgatga tcagatcttt ccaggccagg
     1621 cagggagtca catcgcgaaa caggtgagcc gacgaacgat cggggtcgtt caggcgaatc
     1681 tcggcccgcg gatacttcac gagccagagc aggcggccgt ctgcggcatc gacggcggcg
     1741 accgcgccgc agttcgtatt gaggtacagc cggccggagt cgagcgtcac gagcaggtgc
     1801 gagaggcaat cgaaatcgcc gccgcccggc gagagtgcag agcaaatgcg ggtccgccat
     1861 ctcatgcggc cggcggggcg ggcgtcttcc gcgcggtcat cgaccacgcc gagcgaagtc
     1921 gtctgcaatt caaacgccgc caggtggagt tgcgtccgcg cgccctccag cttgcgcatc
     1981 gccacataga tcgtcccgcc cgccgccagc ggcgtaccct caaacgacca atcgaggctc
     2041 tccggacgaa tcggaaatcc ctccagcggc ttgccctgcg tcgccagatc gaggccgagc
     2101 aaaaagccct gttccttcgc caatagcggc tgattcatcc gctcgcgagg cgcagtcagc
     2161 ggcgagccca tgcgggcgaa caatttgccg ccgccggccg agagcgtgta acgggccact
     2221 ccgatgtgtc ggaccagccc ggcgtgcgcg tcgctgacct cccacgggcc cgccgcgtcg
     2281 tcgcgttggg gcgacaggcc gcgccgataa tcgacttgcc agaccggctg ccccgtcttg
     2341 aggtccaggg ccgccacgta cgaattgccc cgagcgtcga gccgcacata gaccttttgg
     2401 ccaacgacga ccgggtgata actgagcagc gacttcatat cgtcgccgac gcgcagccgg
     2461 ccggtgccca gccagtcgcg gtccgacgtc agccgcggaa gttgataagt ccagagcggc
     2521 ttgccggcgg gatcgaggtc cgcggcgacg gttttgctgc gggcctcgtt gccgccaaaa
     2581 gtcggccaat cggtggactg ccgcggcggc ggccaggacg cggattcttc caacaagctg
     2641 cccagcaaat cggcgtagcg gccttgccgg ccggccagcg ttccctccgc cgtgggcgat
     2701 acggctctta ggatggccag ctcgatctcc gcccgccgcc gcgaaccctc cagcaagctg
     2761 gccagaacca gccgcgcgcg cacagccgcc aggtcgagat ccgtgtcggg atagatgccc
     2821 gcggccggcg gatccgcgac ttgcagcaat gccgccagct cgggtccgcg ccccgcgaag
     2881 tcgaaattcc ggagtggcag ccagagcgga ctccccgcgg gcgtgcgcaa cgccgcggca
     2941 gcctgcggcg gtacggtcag gttcgggccg aggcgttccc agaactcgcg ggccagcgcc
     3001 ggctcgccgc gcgacaaggc cagatcgccc agcttcagca gcgcgtcatc ggccgcccgg
     3061 ctagcaaagg cctgctgcac gacgcgccgg agcaacgcct cgtcattgtt tttcgctccc
     3121 tgctggagcc acccctcggc caacgaatct acgagccggc gatagtgggc gagcgcttcg
     3181 ggcgcagtcg cagccaggct cgccagccgc cattggcaat attcgctggc gggcacatag
     3241 cgttcgaaac cgccgacggg ccgcatcagg ccgaccttca ccagccgcga agcatcggtc
     3301 tcttgcacgc ggcggatcgc ctcgacggct tccgcccatt gcttctcggc cagaaagcgc
     3361 tgggcattct cgagatgggc cgcactggcg gcgtcgagaa ggtccacctc cgccggcggc
     3421 agagcgggcg agcgctccag gatctgcgcc tgggtagcgc gggccagcag caataccagc
     3481 gctagtacta atagcgcggt aggcgggggt cggtgatcga gagtcagctg ttgaatctgg
     3541 cgcatcgtca ccactcacca ccgactcact cgccaggcag ctttttccca gcatacccct
     3601 caaccacacg ccccagcgag aacagcacca cgccgaagac catcagcagc aacatcacgc
     3661 tgacgctgac gttgccggtc ggtgcgcgga ggccaccggt caactgcatg accatcgcca
     3721 gaggcagaat cgccagccct gcggcctgca acagccggcc aaaggaatac accatctccc
     3781 gattgtacgc tgcggagcgg tcgcggccac ggcgcgtcgc ctgaaaccgt caagtagaga
     3841 taaagacctc gtccatctcc ttctcgtcta cgtcctgcct cagcagcaaa tagatggccg
     3901 atgccacggt gaagaaatag gtgaagcgga agccggccac gacgccgtga atgacgcaca
     3961 gaaccagtcc gatcaagacc gtccccaacg aaaaggccca gccttgattg ggcggcgcgg
     4021 gaagacccag ggcgaattgc aatgcggcga agcggagggg ctcgactttg gccgccgtcc
     4081 cgccccaggc cagcgcccaa aagccgaact cttgcaagag cgtcgccgcc ccggaaacga
     4141 ccgcccagca cagcgcccca aacagcgcag ctacgacgac atagaagaaa tagtgcagcg
     4201 gtttaccgta cacgtacgaa tacgaacggc tgaaggcctc gaaggcgtct ccgtcgcgct
     4261 cggcgctgat cgtgggccac attagcggaa agccgaacaa caagccgccg aacagccaca
     4321 agacgcccaa gctggcgatg gcgacgaaga tccaggccag gacggccagg atgctgcccg
     4381 cccccggggc aaattggatt ggcaggccga ggatggcgat ccccagcgcg acgatcaaca
     4441 cggggaccag cgggtagagc ggcgccagca cgtaccagtg ccagcggcgg gccgcgaagc
     4501 gggccgtctc gacgatcccc ggcggcgatt ccgcagcaag gtttacgact gcgtgtcgcg
     4561 atatcactgc gccggggaac gaccacagcg ccagcgtcca caagaagccg aacgcatagt
     4621 aggccatttc gcggagcgta attcgcatgc gaaacagccg agccagcggc tcggccaatt
     4681 gttgatacgc ctcaagaaat gcggtctgtg cgcctggcag gtactcccgc acagccggtg
     4741 gcgtccactg cgcaatgaac gaattctctc cggccgcctg gtacaccggc agatttgtcg
     4801 ttccgctttc gaacttcggc tgaaacacca gccccgccaa gcgccagccc agcggcgcga
     4861 tcaacaccgc tgccgtcgcc agcgcgagca gaggcgggga aatcgcgatc cggaacgtcc
     4921 gcagcaggat caaccaggga aacaggtcac gccaggagat gcggcggatg gtgtccgtca
     4981 tgggctggat gcctggcgcc tagagctcgt gtttggacat ctcaaaacac caaacacgaa
     5041 acactaaaca cactgccgcc cgattgtcca tcccattatc gctcgtcctg gggcggcgac
     5101 tcctccgctt cttcgtctgg atcccgctcg accgggaccg aataagcttc ccgcagccag
     5161 cggccgaggt cgatctgccg gcagcggtcg ctgcaaaacg gcatcgcctg gctcgcagcc
     5221 ggatcgaatt gccggtcgca gatcgggcag cggagcaggg acatgtcagg cagcccgttc
     5281 aatcgacaat gttgagcttg atctcgtcgg agttgccttt caattcgggt gcgtacatgg
     5341 ctgacgcgcg cgtgggcagg gcggcgaatt tgccggggac ttcggcccgc aggcgatagt
     5401 tgaccgaatg cttgccgcgg gcgagccagc ggacgaagaa agtcacgcgg ttgtcgcggt
     5461 attcgacgta ggccggcaag cccttggttc cgtagccgct ccgttgctcg accgcctcga
     5521 agccggccgc tttcatgtcc tcaaagagga ggtattcgta gtcgttcttc gactcgataa
     5581 ccagctcgac ttccaccagg tcgccgctct tgagcgtgtc gccctctttc agcagttgcc
     5641 gctcgtattt ctcaaccttc tgatccagcg gctgcccacg cgtgccttcg accttgacgg
     5701 ttttgtccac ggggacgagc ttgtagtact gccgctcgac tttcacctcc aggccagctt
     5761 tcgtgatgtg gtcctccagc gtgaagttgg tcaggtaggt gttgaaatag accgggccct
     5821 tgccgcggcg gcggagctcg atctcgtgag cgccgttctt cacctcggcc ccactcagca
     5881 cgaacttgtt gtcgtacgta aacaagttct ccttggtgat ctgcacctcc ttgagcttcg
     5941 cgccgtcgag ccagacctcg acgaacatgt ccggctcggt ctcgcccgcg gcctgcaaat
     6001 agtcggcaaa ggcctcgacg cacgtcgcgg tgtcgcgagt gctgttccag tacgttccgt
     6061 gcttgcggtt gttgagcaga tacttgacca gccgcggggc tttctcgcct ttgggatcga
     6121 ccttggccag cagcttcagg taataggcgt tggcctcgac ctcgctgccg taccagtacc
     6181 accaccaatt gttctccggc agcttgaggt acgccgattc gttctcgggg tcctcgacca
     6241 ggaactggct gacgttctgc acgatcatcg ctagcttctc cttgtcgccg accttgtcga
     6301 gcgcgacgcc gaacatcgcc ttggcataga cgggcaactc gatccggtcg cgatacagcc
     6361 ggtcgcgcat cagggcgttg tcgctcgcgg catcgaccag gaccatgtag accatggcgt
     6421 cgagcgcgtc ggcatattgt ttcttgggta cattctcgag cttctgctca aagtttttga
     6481 gccactggag ctgctcggcc tgatagcgct ggagccattc gacgcctttc tccacgacgc
     6541 cggggacgag ggctacgtcg ttggccttgg cgatttgcag gccgtgtacc acaacggcgg
     6601 tcgtgtgcgg ccagctcctc tcgccgacgc cgctgaacca gccccagccg ccgtcgctga
     6661 tctgctgctc ggtgagggct ttcacgccct ccttcaccat gcggctgagc tcagcctcgt
     6721 caaacacggg gttatggtca aaacgcttcc actgcttggc ccgctcgcgg tcgtcgccga
     6781 tctcctgggc gttgaggttc gtccgcttgt cgcggatttc agccaagttg aggttcatcc
     6841 gcatcagcgt cttctgcgtg atgacgctcg gcaggaagcg gttgagcgtc tgctcggtgc
     6901 agccgtaggg atactcggcc aggtacggca gagcatcgac catcgccagg gccagcgaag
     6961 gggagtagcg gatttcgagc tgcgactgat cgacgcggcg ctcggccggg acgctgatcg
     7021 caagcttcga cgaatccttg tctggtcgga tcgtgccggc ccaactttcg gtcttgagca
     7081 tgccgtggat gtagctcggc agcttcatct ccatcgcgtc ggactcttcg tcggtgaggg
     7141 ccttcatgcg gacgatcgcc tcgccctcgt tcagcacctt gcaccgccaa tcgacgcggg
     7201 cctcgccgtt cgccggcacc ttgacgactt gcgttgaatt cacgccgtcc agcggctgca
     7261 aaacgccgcc ggggacttcg agcatgacgc tgacgttctt ctccgccgcc aggtagttgt
     7321 gcacgtttgc cgtcagcacg acttcgtcct tctggacgaa gaaccgcggc gcttgcaggc
     7381 ggatgatgag attcttgcgg gtgaccacgt cggcttcgcc gctgccgacc ttcgtgccgt
     7441 ggcccatcgc ccagacccgg atcttccagg ccgtcaggtt ctcgggcata tcgacttcga
     7501 tttcgccgac gccagtcttg tcggtcgtga gcgagccgac ccacttggcg gtgtcggcaa
     7561 atttcgtgcg gacagccgct tcgactagcg ggccgccctg ttgctggcct tgctggaact
     7621 cggcttcgcc ggcgcgcttg cgctgctcgc tgaagaggcc ttccgccgca aagttcgctc
     7681 ggtcggccgc tttgtcctcg ccaagcggcg cgccgggtgg cgcagcggcc atgggggctc
     7741 cttcggcagc cattccgcga aggagagcct ggcggccgcc tccgccgaag ccaccgaggc
     7801 ctccgccgcg attggccaag ccgttcgcgt tgccgtcggc cttggcaagt tgcatgttgt
     7861 caccggcgtc caaatcgtcg gctacgctcg ctccaaaaac gccgatgaag gccatgctct
     7921 gcttgccggg cagagccagg ttgtacgacc atttcgccag gctgtcctgg tgcgagggat
     7981 ggtggtgccg tcgccattcc cagaagaact tcttgatgtc gccgacattg ctgccgcccg
     8041 agatgtattc gacggacttg tcatacatcg tgagaaccgt cgagccaacg aagttctcgc
     8101 cgttgacgtc agtgagatgg attttgactc tgcctttctc gccgggctta tactgctcct
     8161 tcgacggcag gactgccacg ttaaggacgc gcttttccgg cgggacgacg atctctttcg
     8221 tctcgctgtg aacgtcaccg ccaaagaccg tcactgcttc gacgaagaaa ttgggcatgt
     8281 ccttcttaat gacggcaatc tcttgtagtg agctcttgcc cttcatacgc aggactttgg
     8341 gctccagata aatgccattc gttggccgca aaaagagcaa gaccgtggca tcggcccggt
     8401 tggtgttgag ttgcagtttg actgactcgc cgggcgagta ctcggccttg tctggcatta
     8461 actcgacgtt gttgaagcga tattccgagc cgtcgaagcc gtcgccgatg atcgtaaaga
     8521 tatatccgcc ttcgatggtg tgcttctgct gatcggtcaa cgaataagaa aggcgatatt
     8581 ggcctttggc agacgccttg agttgctgcg cggccctgcc ttccgcatcg gtagccagcg
     8641 gccactgctg gaggggcgtt tcgatcggtt gctgcttgtc gtcgtagctg atcttcaata
     8701 gcttcagcac gccctcgccc ttgaccggct tctgatcgag cgtctgggcc gagaaactgg
     8761 ccgcgatcgt gtcgccgacg cggtagtagc cccgatcgac ccacgaaaac accttgaacg
     8821 gtttgcgggc cacgagcacc ttgccttcgc caacaatcgt ccgccgggat tcatcgcgga
     8881 cctcggccgt gatcgtataa cgatggtcgg tattgccgtg tagttccttg gcaacctgcg
     8941 tgtcgaattc gacctcgatc gtgccttcgg ggccgatctc acgctcgacc tcggctacca
     9001 cctcgggcgg cgtaacgttg ccacgcggcc accagatcgg cgtcggccgc aggcagcctg
     9061 cccaatctct gaaccccttg taccagggat agtcgtaagc gaaccaccag tatcccgggc
     9121 catagcacca gtcccagcgg gcaagcggat accagtcttg tgaatagtcc gtgcggagaa
     9181 ccttgtattt caccgtcgcc ttggtgaccg gcgacccaaa atagtatttc gcctcgatct
     9241 tggcggtaat cttctcgcca agcatcaccg gctcggtcgg cgctttgatc gtgacttcaa
     9301 attccggctt cttatattcc tcaacgcgga agtgatttcc ctcgatggct tggagcggca
     9361 gattgtgtcc ctcgtcgagc tgaatcatat attgaccgag cacggcgtcc gccggcagat
     9421 catactcgcc ttccatcccg ccaaacgcat cagccatgag cgtcttgctg agaatcttct
     9481 ctccctttgg gttgaagatc gcgatcggca acgtcgtgcc ggcgaagtcg ctcttgtcat
     9541 tgtcatattg cgcccgctcg acccacagct tgaattgcac cttttgattg ggacgataga
     9601 ccgggcggtc ggtcatcgtg aagattttga cctggttata ttccgggtcg taatactggc
     9661 ccgtccagac tcccatgaag ccgagaaacg ccagccgcgg cgtcgctccc ttgccgcgag
     9721 cgacgacgag ccactgaaac tccggcttga gatcgattgg gttgggtatg agcaggccgt
     9781 cggggcctgt gagctccgca aagttggtcg tgacgacctg ataacggttg ccccccaagc
     9841 tccgctgctg ccagccgaag aactcgacgt tgatctccgg gattggtgcg ccgccggcgg
     9901 catcggcaac gaagtagagg ttcttgccgc tgagctgctt gtgcacaatc gccgtgtcgg
     9961 cgacccacag cacgatcttg ctgacgttgc ccccctccat gctccccgtg agcaggtagg
    10021 ccccggcttt ctggagcggc gttgagactg tgatccgccg gtcgaaatga ttcggccgcg
    10081 gctccaggtc cagttcccag gcggcggccg cttcgccgag atacttggtc tcgttttgct
    10141 ggacgagccg atagccgata ttgccgaggt tgatcttgtt ccagtcgagc cggttggccg
    10201 ggtccgactt gagataggcc ttcaggtcct ccaggagcag atcgaccttg atcgcccgcg
    10261 cgtcgaactt caccttcttg ccgttgcgga accggaaatc gacggtcgcg ccctggcccg
    10321 ccggctgcga gttggtcgct tcgaacgtgc cccagttgcc gacgatctgc tcgctgcgaa
    10381 gctgcttcca gttgttctgg cccggaccga acttggcgat gctcgtcttc cagtactcgg
    10441 ccgcccgcgg atattgccgc cggttttcga acacttccgc cagctgctgg agagattgtt
    10501 cttccagtcc cgttcgcggc tcggcgatga tctgctgata gagcttgatg tggttgaatt
    10561 cgtcgggcag ctcgaaccgc ttgatgccgc tggcgagttt ggcaatcgtt tcgttttcct
    10621 tcagcgtgtg aagagcgtac gtaccactct cgtccttctt ggtgtcgtcg tccgattgcg
    10681 gcgcgaagaa gccgcggccg aagccgcggc ggccctgcgc catcgattgc acgccgaatt
    10741 gctgctcgaa gaactgcgcc aagtgatagc gcacggcgtt cagccgcgtg ggcgagttct
    10801 cgatcgcctg cgacaagacc caccgccaac gctggccgtc ggtcttcgag gcttcccagc
    10861 tggccggcac gatgtgataa ttcggcttcc cctcggcatc gacgggcgcg ccgttgtagt
    10921 cgcgatagta gaaatagccc tcgtcgaaat cgggcagcgt ggtcaggtcc gtcaaatact
    10981 gaagccgcca ggcttcgtaa tagccgcgat tattgagcag cagctcgccg tagttgagga
    11041 agaactgcga gacttcgtcc ttgcggtcgt ccttctgggc caatggcata gcctgcgtca
    11101 tcaactggag cgcacgtacg cggtcgcgct cgacggagtt gatcatctcg ccgccgccgc
    11161 ggtgcggccc gcgctcgtac tgaccggcga tgcgaaagcc ctggtgctgc gtctgctgat
    11221 agagctgcgc cgcagtctgg agcaatcgcc aattgccctg atgcgtcttg accgtgctct
    11281 cgaccagttc gtcaaactcc ttgacgcgcc ccaaccggtt caggcactgc acggcgttgg
    11341 ttaagtcctg gctgacctgc gccgcgccgg cgttggggtt gagacacagc cggcggaagc
    11401 cgtcgtaggc ctctttgaag ttgccgtttc tcatgagcgt ttgggagctg gtccgcagct
    11461 cggcgggcgt cggattctgc gacgaggcca ctgccaccag gccaacgaga aataaccccg
    11521 ctgccgccag ccgaagaatg aacgtgcttc gcattgccga ttgccttgca gaagagacga
    11581 tggcactcaa attccccgct gcccaagtgc cgccgagtag ccgcgaaggg gggacgagat
    11641 tgagacgagc cggcgggctg cttggatttc cgcaagtatt gtcctggcag ggactttccg
    11701 ccagcgggcc acgcccagtc ctcccacccg gagggggccg taagtacaat cgccgccgca
    11761 agtcgtttga aacagagaaa cgagcacagc cagttttcgt aagccgttgt ctgccaatac
    11821 ctttcggcgt attctggtat tggcagtgtg gcaactgaaa tctgcaggaa aggatcggcg
    11881 tggcggaacc gttcaatccg tatcgagagt ggctcggctg gcacggtgaa tccgcgcccg
    11941 atcattacca gttgctctcc ctctcccagt ttgaaacgaa tgccgaggcg atcgcggtcg
    12001 cgggcgatcg ggctgcgagc aaggtccgca gctttcgccc gggccctcag gcccgtgatt
    12061 ggtcgcgact actcgatgag gttcagcgtg caaaagagtg cctgctcgat ccatcgagca
    12121 aggcccgcta cgacgctcag ctccgcggga attccagtcc gccgccaacc gcggcacagt
    12181 ccgaaccacg aatgtcggga cggccgacca ctggggataa cgacctctat ccgccgggaa
    12241 tgggccggtc ggcaagccaa tcctcgaagc agcaaccgcg ggcgttcgct gagccttccg
    12301 cgcgaaatct ggagccagtc gaatcccccg cctcgccaac cacagcctcg gcgccggccg
    12361 aaattgaacc agcgctccca ccggcggcct attcgcccgc ctatggtgcg cagctcatgc
    12421 aggccacgcc tggcggttgg gcggcaggtc cgtcaccggc acaccaggcg gcaccgccag
    12481 gctatccagc gcctcagtac ggctatgctg ctccagcgtc gatgccggtt gccgggttgc
    12541 cgatggcact tccggccagc gcgtattcac cctatgcagt gcccgcacca gcgacgcctt
    12601 acgcggctca atacgcgggc ggttttcctg ccgcatatcc gacgactgca ccgccagccg
    12661 tcgcaccgcc gcccttgggc agtcttccgg caagctatgg ctcctacggg ggcgcagctg
    12721 atccgatggc acccgtggcg attcaccagc cggcgatgcc cgcggcgatt ccgtcagcaa
    12781 gcctgatccc ggcatattcg ccagcaagcc cggcacctcc gcccgcaacc acggcaattc
    12841 cctttggacc agccgcaact ggggcggtgg gtggtgcggc ttcaccgctc aaagttgcga
    12901 gcccggcgca gccctcctcg acggttctcg ccctggaacg caagcggcac catccgattc
    12961 tcatttcggc cgcctgcatc ggcgtcgtgc tgatcacggc ggtcgtattc gtcgtcgtcc
    13021 tggccaatca gcggcgtgac gccactcttg cccaggtagc gccgcccgcc ccagcacctg
    13081 aagggccgaa gcccgagccg cgcctcggtg agagcgcgaa aacgggccaa ccgaagccgc
    13141 cgcccgaagt tgagcagcca gtcgccaggc caaatccgac gccaatgccg ccggagccgc
    13201 cgccgcggcc catgccagaa tcgccgccga ccccggttgc gacgcctact cccgcaccga
    13261 tgccatcacc gccgccaatg ccggcgccca atcccacgcc gcccgtgacg ccgcagccga
    13321 tgccgactcc ggcgcccatg cccacaccgc ctccgatgcc agcgccgcct ccgccgccgc
    13381 cagcgccgat ggccaccaaa gcggaaatca cggcgctcat caaggcgctc gcggcggcaa
    13441 agggggcgct ggctgaccag gacttcgccg aagccgacca acaattggcc cgcgccgaaa
    13501 acctggcgaa gttgcccaag catcaagacg ccgtcgcccg cctcaagctc gtgggggcct
    13561 acgtcaagca gtttcgcgat gcagtcgcta ctgcggtgtc caacttcgag gccggcagcg
    13621 tgttcatggt gggcagcagc acgcaagttg ccttcgtcga gggctttccc gatcgcgtca
    13681 cgctgcggat cgcgggcatg aacaagacgt atcccttccg cgacatgccg ctgggactag
    13741 ccttggccat cgccgatcag cggctgcttg aatccgaccc ggttaaccgc gtggtgaagg
    13801 gttcctacgt gctcgttcac aagcgcgccg acagcgagtc gcacgagaag gccaagacct
    13861 ggtggaccga ggcccagacg ggcggtgtgg acatgtcgcc gctcatgccg tttctgacgg
    13921 acaactacgc cgagtttctg aaggacgcca gcgacgcgaa gcccgctgcc agtggcgacg
    13981 cgaagccggc ggcgagcccg cccgccgcgc agtaggccgc cgcgattgac tctccgccct
    14041 gctgccttag agtgaggatt cagagcacct ccttcggcaa gcattctctg tggattcccc
    14101 ctccagagtc gccatgcact cgctggccag cacgcgcctc tcgcgcgggc tgtcgctagg
    14161 tttcggcttt gtgcggcgga atctgtggct ctggccgctg ctggctgcgg cgatgctgtt
    14221 tgtcgcgggt cactggattc gcaactcggt cgagtcggcg gcccagatgg aaatggccga
    14281 agggctgaat gccacgctca cctcgaacgt cgaggcgctc aagatctggc tcgacagcga
    14341 agaaagccgc gctcgcgaag cagcgctcga tccgggagtt cgcagggcgg cgggagagct
    14401 ggcccgcctg gcgagctcgt ccggcgacga tccgatcgcc ctggcccaat cgccgcttca
    14461 ggaggaattg aaagccgccc tgcggacgtt tacctctggc cagcggtacg acggctatgt
    14521 cctgctcaac aaggatcagc tcgtccttgc ttcaaaccgg ccggagctga tcggccaggt
    14581 gcggaccacg aattacgacc tcaccgcgcg gacgctcgac aaaggagcgc ttgttacccc
    14641 gccgtttccc agcgtgctgc tctcgacggg tcccgacggc gtggcccgag ccggccagcc
    14701 gacgatgttt gccctggccc ggcagctcga tctgacgggc gatccgatcg gcgtggtggg
    14761 cctgcgaatc cggcccgacc gcgatttcgt tcgcatcctg ggaatcgccc ggcccggcag
    14821 ctcgggcgaa acgtacgcct tcgacaaaga cggcctgatg ctctcggaga gccggtttac
    14881 gccggacctg atccgcatgg ggctcgtccc ggaccagccc ggagcccggt cgcagctcac
    14941 gttgtcgatt cgcgatccgg gcgtcgatca gacaaccggc gcgcgtcccg ccaagacccg
    15001 cgccgagcag ccgctgactg tcgcggcggc cagcgccgtc ctgggacagt cgggctacaa
    15061 cgtccagggc tatcgcgact accgcggcgt gcccgtcgtc ggggcctgga catggctggg
    15121 cgaatacggg attggcgtcg tcaccgaaaa ggacgtggcc gaggcctatc actcgctcta
    15181 tcttttgcgg cgggcgtttt ggagcctgtt cgggctgctc gctgccgcgg ccgggttgat
    15241 ctttgtcttc acgctcgcga tggatcgctt gaaccgggca gcccagaacg cggcgttggc
    15301 ggccaaggaa ttgggccagt atcagctcga agaaaagctc ggcgagggcg gaatgggcgt
    15361 cgtctatcgc gggcggcacc gcatgctgcg gcggccaacc gcgatcaagc tgctgcaccc
    15421 cgacaagacc accgacgagg cgatcgcccg cttcgagcgc gaagtgcagc tcacttgcca
    15481 gctcaatcat ccgaatacca tcgcgattta cgactttggc cgtacgccgg aaggcatctt
    15541 ctactacgcc atggaatttc tggacgggat cacactggat acgctggtgg cgcgattcgg
    15601 gccgcagccg gcgggacggg tggtccacat cctgcggcag gtgtgcggct ccctggccga
    15661 ggcccacgaa atcggcctgg tccatcgcga catcaagccc gcgaacatca tcctcagctg
    15721 ccgcggcggc gaggccgacg tggcgaaggt cctggacttc ggcctggtca aggcggtcga
    15781 ttccaagaag aacgcctcca tcacttcggc cggcgctgtg ctgggcacgc cgctcttcat
    15841 gtcgcccgag tcgattcaat cgcccgagac gatcgacgtc cgcagcgacc tctacgcgct
    15901 cgcggccgtc ggctatttcc tggtcacggg ccagccgccg ttttccggcg cgagcgtcgt
    15961 ggaaatctgc gtccaccacg cccgcacgcc gccgatgagg ccgagcgaga agctgggcct
    16021 gcccgttccc gccgatctgg aggatgtact gctcaagggg ctggcgaaga agccggaaga
    16081 tcggttctcc acggctcgcg aattcgccga ggcgctcgcg gcctgcgccg acgcagccgc
    16141 ctggaaatcc agcaccgccg acgcctggtg gcgcacgaac tatcccggca acgcggccgc
    16201 caccatcagc atcgaccaga ccaacagcat tcacggcgag cagacgctga tcacgaccgc
    16261 gccggcgctg aaataaattc gttgatctac tatctgcggt agaaccccgg ctgcgggaac
    16321 cagacgaagc cgagctggaa cgtgcccagc cagttgttat ggaagtcgta gcggccaatg
    16381 ggctggacga tgccgcccgt ggtgcgaaag tcatcggtct ggaagtcgcc gacgtagagc
    16441 acggcaaacg acatgtccat cgccaggttg ggccagatcc agaagtgcgt gcccagggca
    16501 aagcgggtcg ccaggcaggc cgtcgtgtcg ccgaagaccg tggcatattg ccccgccggg
    16561 ttgccgtcgg ggaattcggc cgtggcggcc agcgtgttga acgccacgcc gaggcccacg
    16621 cccgtgtagg gatcgaaccg cggacggttg cagtgctcgg ccggcgggaa gaagtgccag
    16681 tacgcgttgc ccagcacgat gttggaggtg gcgtcgctgt cgaagccggc aatctgcgcc
    16741 ccgccgaaga aggcggtccg ccactgataa tcgccgtgga tgttcgtgta gctcacgtcg
    16801 gcccgccagc gcgggctcag gcgcaggccc agccgcagag tgaaattctc gccgccgtgg
    16861 agctgcgttc cgaaagccac gccgtcgaag aacgcgccca cgccgttgga gtcgggtgtc
    16921 acgttcagcg gaatggtcca gccgccctcc acgccgacat accaaggctt ccactccttg
    16981 caccaacagg gcggaaacgt cggataggga ggatgttcgc cgccgatcgg cacgagcggc
    17041 gcctcctcgc ccgccggcat ttggccgccc agcaaccaga tgctcgcgtt tgccggctgc
    17101 tcaacggtca ctgcggacag atcgatcgtg tcggcgggcc attcttccgc cgccaagggg
    17161 caggccgccg tcagcaggat ggcgaccgcg gcggcgtggc ggcgaatgga tgtgaacatg
    17221 taacggtccc gcagacattc ccggcacgag aaccgcgccg cccggcacag ccgctgcgcg
    17281 cggcgcatgc cagagtatcg gaactcacgg cacgaccgcg ccagcaccct cggtttccgc
    17341 ggcacaaacc ggaaagcccg cagagtcggc caaagccgcg accagtggtc gcggcctcgg
    17401 cgcgcgacca atggtcgcgg ctttggatta ctttcgcagc gaagccagcg cggcgcggac
    17461 gctggccagc tgctcctgga gctgggccag gctgtcgcgc tcggcctgga cgacgttggc
    17521 cggggcccgg ctgacgaagc tctcgttggc cagcttgcct tccttggcct
//
LOCUS       DASRJB010000161         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2015685, whole genome shotgun sequence.
ACCESSION   DASRJB010000161 DASRJB010000000
VERSION     DASRJB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015685"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VFB80_04535"
     CDS             complement(<1..1121)
                     /locus_tag="VFB80_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN33058.1"
                     /translation="MQLRLPLPILQWLPKYERAWLRFDVVAGVTLAAYAIPVSLAYAS
                     LAGLPPQMGLYCYLVGGLGYVLFGTSRQLAIGPTSAISLLLGVSLAELAGGDLVRLAH
                     LASLTALLMAGVFVLAWLLRFSVLVNFISESVLTGFKAGAALVIAATQLPKLLGVKGG
                     GDDFFERIWLLVGQLNATHLLTLAVGLAAILLLVAGSRLLPGRPVALVVVFLALLAVW
                     LGGLDQRGVKIVGDIPAGLPQFQWLGGELAALPASAVRQLVRLAAACFLLSYIESVSA
                     ARTFALAHQYEVNPRQELLGLGAASLLAGLFQGFPVAGGLSQSAVNDRGGARSPLSLV
                     FASAAICCVLLFLTGLFRTLPDAVLAAVVLVAVSGLIDIP"
     gene            1319..2257
                     /locus_tag="VFB80_04540"
     CDS             1319..2257
                     /locus_tag="VFB80_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN33059.1"
                     /translation="MTRSFLAPLSLALLTVLLGAASSLAQPPRPKAQPAISADDQQVR
                     QGVVAFVQQFNAHKADAVAALFMPDARMTLRDGTQFDGRDEIKKSFEAAFAESPKTAI
                     SVVVDSIRFLTPDVAVEEGSTSSFPDGETLTARSGYTVVHLKKDGRWLMQSVRVVEEE
                     SLSAYGDLQPLEWLIGEWIDEGRAESVHTTFRWDDNKSFLLEEFQVVREGSVVLKGSQ
                     RIGWDPQAKQIRSWTFDSAGGFGEATWTPVEEKWICKARGITADGASASATRTLTRAT
                     ADRVIWTATDRVAGGEQLPDLAVTMVRKPPKAQIQQ"
     gene            2301..>2967
                     /locus_tag="VFB80_04545"
     CDS             2301..>2967
                     /locus_tag="VFB80_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33060.1"
                     /translation="MSRELKQATSVLLIVLMTLGPTSFSYGRGGGRGGGGGGRGGGGG
                     GGRVGGGGGARVGGGGMARPSSPSFSRPSMPASRPAGGISRPSPGPRPSNPIARPSPG
                     LTPGSRPGTVDFGRPGSPGLTPGTRPSIGGPGISQLPSTRPGVGAPGVGNPGIGSRPG
                     VGAPGISQLPAGRPGTGGRLPGGSTALLPGMGNRPGAGGAGRPSQLPNRTPQERREGL
                     QDRL"
BASE COUNT          555 a          989 c         1004 g          419 t
ORIGIN      
        1 ggaatgtcga tcaaacctga cacagcgacg agcacgaccg ccgcgagcac ggcatctggc
       61 agagtgcgaa atagcccggt cagaaatagc agcacgcagc agatcgccgc ggacgcaaaa
      121 accagcgaca gcggcgagcg agcgccgccg cggtcgttga ccgccgactg cgacaggccg
      181 cccgcgaccg gaaagccctg aaagaggccc gccagcaagc tcgccgcgcc cagccccagc
      241 agctcctgac gcggattgac ctcgtactgg tgcgcaagtg cgaaggtccg agcggccgag
      301 acgctctcga tgtaggagag caggaagcag gccgcggcca ggcgcacgag ctggcgcacg
      361 gcggacgccg gcaaagccgc cagctcgccg cccagccatt gaaactgcgg caggccggca
      421 ggaatgtcgc cgacgatctt gacgccccgc tggtcgagtc cgcccagcca gaccgccaga
      481 agcgccagga agacgacgac cagcgcgaca ggcctgcccg gcagcagacg cgagccagcc
      541 acgagcagca ggatcgcggc cagcccgacg gccagcgtga gcaagtgcgt ggcattcagc
      601 tgcccgacga gcagccagat ccgctcgaag aagtcgtcgc cgccgccctt cacgcccagc
      661 agcttcggca gctgcgtcgc cgcgatgacg agggctgccc cggctttgaa gccggtgagc
      721 acgctctcgg agatgaagtt gacgagaacg ctgaaccgca gcagccacgc cagcacaaac
      781 acgccggcca tcagcagggc cgtcagcgac gcgagatgcg cgagacgtac caggtcgccc
      841 ccggcaagct ccgccagcga gacgccgagc agcagcgaaa ttgccgacgt gggaccgatc
      901 gccaactgcc gcgacgtgcc aaacaacacg taacccaggc cgcctaccag gtagcaatag
      961 agtcccatct gcggcggcag accggccagc gaggcgtagg ccagcgaaac cggaatggca
     1021 taagccgcca gcgtcactcc cgcgaccacg tcgaacctca gccacgcccg ctcatatttc
     1081 ggcagccact gaaggatcgg cagcggcagg cggagttgca tgttcatcgc gacttaagaa
     1141 atttcaggag cttgtcaatt atcgttggcg cgcgtgtccg atgcgattgc cagccacgtg
     1201 caattcgagt tgccgggcat tagactggga acactctttg atgcaattcc cggctgtcga
     1261 gttgctcttc tctcgctgcc tggtgatacg acgaccaaga ctcttctgga gcatgtccat
     1321 gactcgctcg tttctggcgc ccctatcgct tgcgctgctc acggtcctgc tcggggcagc
     1381 cagcagcctc gcccaaccac ctcgtccgaa ggctcagccg gcgatttctg ccgacgacca
     1441 gcaggtccgg cagggagtcg tggcctttgt gcaacaattc aacgcgcaca aggcggatgc
     1501 cgtggcggcg ctcttcatgc ccgatgcccg gatgacgctt cgcgatggaa cgcagttcga
     1561 cggtcgcgac gaaatcaaaa agagcttcga ggcggcgttc gccgagagcc cgaagaccgc
     1621 gatcagcgtc gtggtcgatt cgatccggtt tctcacgcca gacgtcgccg tcgaagaggg
     1681 cagcacgagc tcgttccccg acggcgagac gctgacggcc cgcagcggct acaccgtcgt
     1741 gcatctaaag aaggacggtc gctggctgat gcaatcggtg cgggtcgtgg aggaagaatc
     1801 gctttcggcg tacggcgact tgcagccgct ggagtggctg atcggcgaat ggatcgacga
     1861 gggccgcgcg gaatcggtgc acacgacgtt tcgctgggac gacaacaaga gcttcctgct
     1921 ggaagagttt caggtggtcc gcgaggggag cgtcgtcctg aagggatcgc agcggatcgg
     1981 ctgggatccg caggcgaagc agattcgatc ctggacgttc gattcggccg gcggcttcgg
     2041 cgaagcgacc tggacgccgg tcgaagagaa gtggatctgc aaggcccgag ggatcactgc
     2101 cgacggcgcg tcggcgtcgg cgacccgcac gctgacgcgg gccactgccg accgcgtcat
     2161 ctggaccgca acggatcgcg tggccggcgg cgagcaactg cccgacctgg cggtgacgat
     2221 ggtccgcaag ccgcccaagg cgcagatcca acagtgacaa tcgcctcacc acaaacaaat
     2281 ctctcaccgg aaaatcggcc atgtcacgcg aactgaaaca agccacgagc gttttgttga
     2341 tcgtgctcat gacgttggga ccgacttcct tttcgtacgg ccgtggcggc ggccgcggcg
     2401 gcggcggtgg tggccgcggc ggcggcgggg gcggcggccg cgtcggtgga ggtggcggcg
     2461 cgcgggttgg cggcggcggt atggcgcggc cttcttcgcc gtcgttcagc cggccgtcaa
     2521 tgcccgcctc tcgcccagcg ggcggcatct cccgtccttc gcctggcccg cggccgagca
     2581 atccgattgc ccgaccctcg ccgggactga cgcccggcag ccgaccggga acggtcgatt
     2641 tcggccgtcc cggttcgccc ggtctgacgc cgggaacgcg gccttctatc ggcgggcctg
     2701 gcatcagcca gttgccgtcc acacggccgg gagtcggcgc tcctggtgtc ggcaatcctg
     2761 gcatcggcag ccggccgggc gttggcgcac cggggatcag ccaactgccg gccgggcgcc
     2821 ccggcacggg cgggcggctc cccggcggca gcacggcgct gcttccggga atgggtaatc
     2881 gccccggcgc aggcggcgcg ggccggccga gccaactgcc gaatcgcacg ccgcaagaac
     2941 ggcgggaggg tcttcaggat cggctgg
//
LOCUS       DASRJB010000162        25119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2582796, whole genome shotgun sequence.
ACCESSION   DASRJB010000162 DASRJB010000000
VERSION     DASRJB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582796"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFB80_04550"
     CDS             complement(<1..318)
                     /locus_tag="VFB80_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33061.1"
                     /translation="MAARAEISSADEILSFRERYRREMSCQVVHDSIHRRPGWMVTYR
                     LASGGAAAGYGSVAVGGPWQDKPTLLEFYVLPEFRRRAFELFEALVAASSARLIEMQS
                     NDAL"
     gene            complement(329..1156)
                     /locus_tag="VFB80_04555"
     CDS             complement(329..1156)
                     /locus_tag="VFB80_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33062.1"
                     /translation="MTGTCSRRLAWLAFFAVVSELAALGQPPPAAEDPAKANEASLAT
                     MKAMAGRYELTAGAKNQFKLDRTAEPVQRWTNPVRGQPDGCLYLWTLAGRPQAALTIY
                     PRLDGTAWNHEFQSLATTPLVAKYDGEEAWTPDQPGVELKAVPGASAPADSAARRLVQ
                     MRAIARGFAATVDVRGDKSALRLLTAPIYRYEGGSGDPLDGAVFVFAQATDPEMLLLL
                     ETRAGGGKSQWHYALARSTLWPLSATYESQPVWSVEKWNRATAHIKQPYLDRPRPEK"
     gene            1394..2509
                     /locus_tag="VFB80_04560"
     CDS             1394..2509
                     /locus_tag="VFB80_04560"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZN33063.1"
                     /translation="MDGNSRNRMGISAIATYEPPWLLANDWFDGVIPRKFVHHTGIEA
                     RPVSQEDEVTMAVRAVKNLQREVGCDLSACAAVVFASPSFIPPAAAHRLGGEQVARQE
                     RLRVAGRQLVRRLGIASCPVAAINWFCSGYSRAVALATRRFAPRLKLQPHQYLLVVTS
                     SRISRITDYSCPQTSALFGDMATVTMLAPLSNQRHPVHFELLYADARKQPADGVFFEF
                     HLRQNVPAPTEEGGREIAAERLVFSLDGMSIADAAPRAMAGALAEALTATGLRPEDLK
                     FVVPHQAGAGIVRLTAMKLDQLGVRAEVINGLTQRVGNISSGSIPYGLKQTWNKLTGT
                     IACPAAAVGSPGVKEVSQGCLLFRATPYHDQIALAVA"
     gene            complement(2510..3016)
                     /locus_tag="VFB80_04565"
     CDS             complement(2510..3016)
                     /locus_tag="VFB80_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN33064.1"
                     /translation="MKAVSRLMLFAGLILLGRTASAVDDVPAKAAPAAVKPVYSVETY
                     DPKRDAAADLKLTIEKAKGKRILVQVGGDWCGWCHLMNKYFHENEKVAAALAKDFVIQ
                     KVNYSQENQNKEFLSKYPAIKGYPHIFVLESDGTLLHSQNTEELEEGKGYSERAVLEF
                     LAKWAPKR"
     gene            complement(3013..5049)
                     /locus_tag="VFB80_04570"
     CDS             complement(3013..5049)
                     /locus_tag="VFB80_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HZN33065.1"
                     /translation="MIDIAHILGPGGRIAARLPNYEHRPQQLEMAAAVEQALFDGQHL
                     VAEAGTGVGKSFAYLAPAIAYVTAEPPAREDDDSDDEDTRPRRRIVISTHTISLQEQL
                     LQKDLPLLRSVIPDEFSAVLVKGRGNYLSLRRMNLAGERSVSLFSQEEELRELRKVIA
                     WSKKTGDGSLSDLSFRPRPSVWDEVASDSSNCLGRKCGTYAKCFYFRARRRIVNAQLL
                     IVNHALFFSDLALRRAGASILPNYDAVIFDEAHTLEAVAGDHLGLGVTSGQVQFLLNK
                     LFNDRTHKGLLASPLYHEAQDLTVECHTRADELFDDAAALTQRPGNRTGRIHESNQLD
                     NRLSPALLKLARELRRAAAEIENESERLDFTSAHDRLTALAGELSQWIEQGAADSVYW
                     IETAASRSGRTRVALSAAPIDVGPVLRDELFAKTKCVVMTSATLAVGRPPQFDFFKSR
                     VGLTSCQTLRAGSPFNYREQAQLILVRGLPDPTAAKEEFERRSLVVIQRYIERTDGHA
                     FVLFTSYDALRRAAAGLSRWLAQQDLALYSQAEGTPRSQLLAQFKQNPRGVLLGTDSF
                     WQGVDVPGDALTNVIIPKLPFSVPDQPLLEARLEAIRAAGGNPFRDYQLPEAVIKLRQ
                     GFGRLIRTQRDRGMVVLLDPRLHTKPYGRIFLQSLPDCTIVEESAAEAKPGVSS"
     regulatory      5321..5413
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5726..5911
                     /locus_tag="VFB80_04575"
     CDS             5726..5911
                     /locus_tag="VFB80_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZN33066.1"
                     /translation="MNKFLKFARNFLVNEDGPTAVEYAVMLALIIVVCLTAIQAIGTN
                     ANATFNSVAGVLPGGAS"
     gene            6196..6762
                     /locus_tag="VFB80_04580"
     CDS             6196..6762
                     /locus_tag="VFB80_04580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN33067.1"
                     /translation="MDLATDYAAVIAEHWTIWLVSVVLIVAAVIDGYELRVPNLITYP
                     FIIAGWVYSTYAFGLQGLGWSLAGTALGMSILMAFHAIGGMGAGDVKLMGGVGAWIYW
                     THTWYCIIGFALVGAVMAIVMIVVSGKWKHHYNQFWKIVNEITTIKDPAKLSALAAER
                     KPNMMLLPYGIPIAIATISYFLWTGMLV"
     gene            6759..7274
                     /locus_tag="VFB80_04585"
     CDS             6759..7274
                     /locus_tag="VFB80_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZN33068.1"
                     /translation="MSKLCTPAIANLLSTVWQAASFGASLPPAKDKLERSCRIFRRKR
                     RAAAAVEFAIVAPVFFLLVFGMIEYGRMVMVQQVLTNASREGARVAVLDGATVATVQT
                     RVNDYLAAGSITGATITVAPNPLSGAEFGDPVTVTVSIGFNQVSWLPSPMYLGGKTMT
                     ASTVMRRETVQ"
     gene            7364..8398
                     /gene="cpaB"
                     /locus_tag="VFB80_04590"
     CDS             7364..8398
                     /gene="cpaB"
                     /locus_tag="VFB80_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZN33069.1"
                     /translation="MRMKSLILIFIALGCGLVAAVGISQVMSGGGSASTLETDQILVT
                     LTDIDIGTKFDAQNVKLEDWPKNKIPDGAVRRFDDVKDKYAASRFYKGEPLHASRVSD
                     MLNNPAVKVPPGFRAMPVKVEEDTVLKAIGPGDRVDVIVFLRRNPPEIMETGAFPILR
                     NVRVFAVNTNTERAADAKGETTNFRTVSLLVKEAHAGELVVAAKMGNIFLTLRRPDEV
                     DSAEGEEVTPIKEVLAGPNRLQSEPGSRGPAPSTPTTLDFVRNATAAPAGGRDPGARP
                     EWSMVLMSPSDWKQFDWADAKGGLPTESRLSAAPPVSAEPLDKTSGSKPEGSDNDNPG
                     TPPPDGHKTN"
     gene            8538..10175
                     /locus_tag="VFB80_04595"
     CDS             8538..10175
                     /locus_tag="VFB80_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZN33070.1"
                     /translation="MSHPSLKVAMLAALGCVLAGAIPAFAQQPAPPGGVKFQVRGPAE
                     RLEMTVNTSRVVEFPFDVPRMLVNNPDLVRVVPISPKSVQLSAVKAGITQVNIWDIDG
                     NVTTLDLIILGDVGELDLTLKSLFPEASLRLRPLNSSLYISGFVPKAEMVGSVIRVAQ
                     DYFPSIINDMTVGGVHKILLHVKVMEVSRTKLRAVGFDWDLVSSGGFVTQSVSQLVPG
                     VFPINADAFPTVRFGITSGDQFRGFLQLLRQYDLAKLLSEPTLTTLDGRPASFNVGGQ
                     IPIPIQQSLGVTTIQWREFGTRIDFVPVVLGNGTIRLEVRPDISQVDSSLRDSVTGTV
                     GFRSRRADTAVEMKAGQTLAIAGLVESRTEATNRGIPILADLPWLGAPFRRVQERNNE
                     IETLIFVTPEFCEAMDPCEVPPCGPGQLTTSPTDCELYGRGYIEVPKNCPTPGPGLMG
                     PSGYEVLPGAGAPVNGGAPLAPPIPPPSPSAGTTGTSRRALAVGTGAAGRPASSGRVS
                     DLPSSSGTSYYSGAGTQSPAPVVKPALIGPAGYDDLR"
     gene            10308..11528
                     /locus_tag="VFB80_04600"
     CDS             10308..11528
                     /locus_tag="VFB80_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HZN33071.1"
                     /translation="MSNVLRLAIVDPNDNNRTQLKNMLLGMDIVWLEAECSRYEFFSD
                     VIGQTKPDIGVVALDNNPEKALKLIEQIRDIAPDCSLLVVSSSTDGQVILKAMRSGAK
                     EFLTQPIKVEDLIGALGRISSAKFGAGDGRNRTCHVITVCGATGGVGATSLAVNLGSI
                     LAKDEKNSVVLVDLDLALGDADVFLDTIPDYTLVDVAQNIARLDFTLLKRSLTKHSSG
                     LYLLPRPVQLQDSSQITADDLTRVLGLLKASFTHMIIDLSKGYTPIDNVALKSARDIL
                     LVTQLDLPCLRNVVRLMLSFNEDEAIKDKVKIVINRVGLDNGSISLKKAQETIGREIF
                     WQLPNDFKTMVEIRNNGVPLIEQAPRAGITQALVALAERLVGEGRSKDEADSNKGAKN
                     TSTGWLNFLGGKKK"
     gene            complement(11535..12740)
                     /locus_tag="VFB80_04605"
     CDS             complement(11535..12740)
                     /locus_tag="VFB80_04605"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZN33072.1"
                     /translation="MDPHLLTRELHTKNDSKIVLLVADGLGGLPMTPGGKTELETARK
                     PNLDALAKIGVQGGSIPVFPGIAPGSGPGHLGLFGYDPIKYLIGRGALEATGIGFHMQ
                     PGDVAARGNFCTLDAAGKISDRRAGRIPTEESSPLAVRLREVKTPGYETFVEPVKEHR
                     FVVVFRGPGLAGNVKDTDPQATGVPPLAAAAQDDASRKTAEIANQWIAAATKLLAGEK
                     KANGLTLRGFAARPNLPSYEEVYGLKAAAIAVYPMYKGLARLVGMEIVGKSQTLSEQI
                     DTLAANWSKYDFFFIHFKYTDSTGEDGNFDAKVKRIEELDAIVPRITALKPTVLIVTG
                     DHSTPSFLKSHSWHPVPTLLVSNCCRTDGHATFGENTAVRGGLGQFEAKYLMTLALAN
                     AGRLSKYGA"
     gene            complement(12749..13567)
                     /gene="trpA"
                     /locus_tag="VFB80_04610"
     CDS             complement(12749..13567)
                     /gene="trpA"
                     /locus_tag="VFB80_04610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZN33073.1"
                     /translation="MSRIDALFAELRKSGRKAFMPFVTAGDPDLAFTADVIRELDRCG
                     ATLCEVGIPYSDPIADGPVIQASYTRALAKKIKLPAIFEMLRGVSGQVKMPLVTMVSY
                     AIVHRYGLKKYVEGAQRAGASGAIVPDLLVEEAGELAAICKAADFSLIQLVTPTTPRE
                     RALQIARASTGFLYYVSIAGITGERTELPPSLVENVGWLKSQTDLPLCIGFGISQPAH
                     VKLLAPVADGLIVGSAIVRRIAEAATKPREIVLTGIGEYAAQLMDALPSPSPQP"
     gene            complement(13575..14789)
                     /gene="trpB"
                     /locus_tag="VFB80_04615"
     CDS             complement(13575..14789)
                     /gene="trpB"
                     /locus_tag="VFB80_04615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZN33074.1"
                     /translation="MTAPATVPTANAVPDSSGRFGAFGGRYVPETLTRALDELTAEYN
                     KARLDARFQADLDELFEHYVGRPSPLYFARRLTAECGGAQIWLKREDLNHTGAHKINN
                     TLGQALLTLRMKKRRVIAETGAGQHGVATATACAHFGLDCVVYMGEEDIRRQAPNVNS
                     MKLLGAEVRPVSSGSRTLRDAVNEALRDWMASVETTHYIIGSVIGPHPFPRIVRDFQA
                     VIGREARRQSLERIGKLPDLVVACVGGGSNAAGMFYPFIGDQSVRLLGVEAGGRGPQP
                     GDHASPLSYGSPGVLHGSYSYVMQDEDGQTCDVHSISAGLDYPGVGPEHSYWKDIGRV
                     TYTFCEDKDALAGFDALARNEGILPALESSHAIFRAMEQARQMRKGESIVVCLSGRGD
                     KDAAEIARLRSG"
     gene            complement(14864..15796)
                     /locus_tag="VFB80_04620"
     CDS             complement(14864..15796)
                     /locus_tag="VFB80_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33075.1"
                     /translation="MSPHSMIAACLCGTLFAAAAWAGDIDRDPPPQESLQLVLDRIHT
                     HAGGAAWKERNWTDAAIEGWIDKLLKQLGEATGKELKAPVRLKDVKPAGDTDQLLRGH
                     LKIGKHVKQSSVYDSIVLADGNAELSTVKNSIVIARSVATTSSVENSLIIAGRYFRCS
                     SDRVVNANPTSSILISRGHGEITIARGTIICAPLGIEVSLAENVTFVNCKVNRALNQQ
                     NVQMVNGKPLPLGEPPVSALEKQLELLGYMPSPPGALFRFQGRRLFAEKDQPIVDELG
                     APVKELAGWSLSHVDLRMALFTSGEQEACLIRAR"
     gene            15872..16150
                     /locus_tag="VFB80_04625"
     CDS             15872..16150
                     /locus_tag="VFB80_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33076.1"
                     /translation="MTDSRPAKARLGCGFLLVSAALTCVMLAINGLIVTNLVAAYSPG
                     PLPLENQRVAQAAVFLGPVVLLFVEWWIFDVALDWLRPQRAAQGRPKA"
     gene            16169..17692
                     /locus_tag="VFB80_04630"
     CDS             16169..17692
                     /locus_tag="VFB80_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN33077.1"
                     /translation="MQPVRCLLTALVALVGSAALAAEPAARPNVVFILTDDQRWDTIS
                     LHPKSVVKTPHIDRLGKEGVYFKSSFCTTSLCSPSRASILSGLYAHTHGVTNNFTEYP
                     NDLPSFPKALQAAGYATAYIGKYHMGEENDSPRPGFDYFVTHKGQGNYFDTEFNVAGE
                     RKVLPGYYTHVVTDLAEKWLRSVSGGGLRGAAGDKPFLLMLGHKAPHSFYFPEPKYEH
                     AFDGEKIEYPRSAFQLEDKPKWFLARLDTWHGIYGPLFEYRKQFPDRRPEAVKDFAAM
                     TRGYWSTILSVDDSVGRLYAVLKELGELDNTLFIFTGDNGLLNGEHGMVDKRTMHEPS
                     IRTPLVVRLPNTLTRRASEGVPRVIDEQVLTIDFAPTILDICGVAPLDKTHGRSWKQL
                     AQGDAAGWRKSWYYEYNYEKQFPYTPNVRGVRTDRWKLIRYPHGDGGPDRHLAELYDL
                     QADPEETVNLVAKPELAASVAELNAELTRLMEAAGGLPDKMPLDEGIKSGLPEKAIR"
     gene            18327..19190
                     /locus_tag="VFB80_04635"
     CDS             18327..19190
                     /locus_tag="VFB80_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HZN33078.1"
                     /translation="MIRRLAQPLGVLALAACVLLTALGGLSHWRGILMGRIGPQHTVL
                     IRSTYGKAQIDIHRTDAREPTLWIWSTWPHEFAFREPNLGGWWRHGGFGLAQGGTRGG
                     NTVQQITLPYWSLTLISLLLASGLLLAGPKQAQQQGVRDWKQSEFADHCNAASTASSL
                     DVELVKRTETKLSRKLPLGYVARMCGDNGGAVAAARDKWSLYPIFDDSDKKRLERTYN
                     DIVRETAEARQCPEFPPDAVAIGHNSGGDKLVFMADAEAGRYADAVYRWDHKTGKLKQ
                     VAAEFEQLKPA"
     gene            19302..19997
                     /locus_tag="VFB80_04640"
     CDS             19302..19997
                     /locus_tag="VFB80_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33079.1"
                     /translation="MLTDDQRKVWEAYEAAERRGPRAEMLQSLKAFLDSLATSPPADW
                     FPWARSLAEQVVDHGLDFYIRQPLFERAVFPALLAGYQARLPGSARWLAGLCQNIYRS
                     PACRQQLPPEESTELGMLWAALRHDPGDRRARVRLIKSIADRLRYSLHELPAGVLYGM
                     DGASPEQCLELERELQEFCGLVAQEDMHETYANLIRDCGFHFRGYRDYLLHRERYQNY
                     GEYIAHNDEMAGG"
     gene            20056..20874
                     /locus_tag="VFB80_04645"
     CDS             20056..20874
                     /locus_tag="VFB80_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33080.1"
                     /translation="MEASLAIHTAARRYCLERYACWAERYAEIVRHGRDRGHDRYHYT
                     SEALATFPRYNVLNAIRVELERIDAGELSDFEAVKSQVLRAGETANDEFTLKPIGAID
                     QRATEEERAAFCSYVARLSPADLYGVEPLPFRRVLAADESESLWSRLRKRWGIGAGCW
                     YPLGECTLPGVEAFDASAFDKAAPPECLRGILSDRRIERVWELREFGPEYELAVALFD
                     PHYDGAEGYWSSGEMDWIVYASHERSVTVGGWLLKEVMSLWPAWQAHLWRGDRT"
     gene            complement(21158..24025)
                     /locus_tag="VFB80_04650"
     CDS             complement(21158..24025)
                     /locus_tag="VFB80_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33081.1"
                     /translation="MLRPLALTILVSAYALAGPASAAPNIASASPRGLQIGQPTTLLI
                     TGSDLPADARVLIAGKIAGQTIKPGAKSDRVEIEVTLDPATSPGLYPIRVAGSGGISN
                     PIVIGVDRLPQVAFETEPKTLPIALHGAVGAAQVLKMTLAGKKGQRLVLDVEAQRLGA
                     GLKPVLRLYDARGTQIAWSPPRAIIGGDARVEALLAADAQYTVELHDQLYRPAGPGYF
                     RLKIGDLQFADLALPLGATTGSKQAVRFASSNIPDVVELDLTANPAPGETASLLPAAE
                     RYSGAAPRIAVSDFPELVENPAAGGQLQELPAAPVAISGLLSAANEEDRFLLNVTPGQ
                     KLRVEVTARRFGSPLDAVLSIRNEQGGSLASGDDRPGSSDPMVEYTVPAGVNRIVLAI
                     KDLLGRGGSDFVYRMVAADTSRPDFSLSLATDRINVPSGGTQVIPIQVTRTNYAGPIA
                     LSLEGQPAELQLQGSVIPAGATIGLLTLSAAPGSPQATLSRLIGQSASQTPPVRRAAT
                     FGDFPGAKYQPRVKTELGLAITQPAPLGLVWLPGDNDQLFLGGKLPARVQLTRAEGTK
                     GKTRLKLLTTQPTPKKTVKENNQDKVVDDLDRALRLEGDATFADDQKDVTAQILVPGD
                     LPRQPWDLVLVAELLSADGKTVVASIAAPVRTLSPVAPFTLALTGPNAAEGKAGAGEP
                     GKLVGKIERSPGYTQSVVVTLDGLPKGYSAPQVIVPADKSEFELPLTFAFGSKAGELK
                     GAKLVGIAAPVVATSVRSNTLDVAINVVAGEKPAAEPPKEIFEDDEKFAALLTEGEGR
                     AIPDQRDKYSGTYSLRITGDQKFNPNLPNLGMKIRENPGPGEYRYIRFAWKKAGGNSI
                     CLQLNHDGTWGPGGTGREGAKFRYHAGPGGECYGASLTIDEKLPAPRFVVVTRDLFAD
                     FGEFTLTGLAFSPVDGQAALFDHLYLARQPEDFSLLKVEKQ"
     gene            24096..>25119
                     /locus_tag="VFB80_04655"
     CDS             24096..>25119
                     /locus_tag="VFB80_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33082.1"
                     /translation="MPRQAGDGPRQAGSVNGLTEQARAREPALRVWAARQTGHPPEQP
                     ALEPARALEQREPSASAAPQTDRRLAVQERARALALVPAREPEQGQPERALEQGPALA
                     SAAQRTGHRPEPRASARALQELALESLAWAARRTGQQPVLQVPARGPVLVRQPALEVY
                     QTDRPARVQAREPGQALAVFQMDPLVQVLEPAPVPVQAQEALQMDPQQAPVRALELVL
                     ARVLVLAREPAEFQTDRLALEPARVQGPALAVYQTGQQGRAPEPEQARVPGQALAQEF
                     QTDPRRAPERALGPVPGWGPGPVLEFLMGQRPVLEPELGQARAPEQVPEPAQVYQTGP
                     RPEWPAR"
BASE COUNT         4315 a         8110 c         8314 g         4380 t
ORIGIN      
        1 gagcgcgtca ttcgattgca tctcgatcag ccgcgcactg ctggcagcca cgagcgcctc
       61 gaacaactcg aaggcccgcc ggcgaaactc cggcagcacg tagaactcga gcagcgtcgg
      121 cttgtcctgc cacgggccgc cgaccgccac cgagccatag cccgcggccg cgccgcccga
      181 tgccagtcgg tacgtcacca tccagcccgg ccggcggtgg atggaatcat gcaccacctg
      241 gcagctcatc tcgcggcggt agcgctcgcg gaaggagaga atctcatcgg ccgacgatat
      301 ctccgcgcgg gctgccatgc tgcaaggctt acttctccgg cctcgggcga tcaagatagg
      361 gctgcttgat gtgggcagtc gcccgattcc acttttccac ggaccagacc ggctgcgact
      421 cgtacgtcgc cgacagcggc cagagcgtcg agcgggccag ggcatagtgc cactgcgact
      481 tgccgccacc ggcgcgggtt tcgaggagga gcagcatttc cggatcggtg gcttgcgcaa
      541 aaacgaagac ggccccatcg agtggatcgc cgctgccgcc ctcgtagcga tagatcggag
      601 cggtcagcaa ccgcagcgcg gacttgtcgc cccgaacatc gacggtcgcc gcaaagccgc
      661 gggcgatcgc ccgcatctgg acgaggcggc gggcggccga atcggcgggc gctgacgctc
      721 cgggaacggc cttgagttcc actcccggct gatccggcgt ccaggcctct tccccgtcgt
      781 acttcgcgac caggggcgtc gtggccagcg actgaaattc gtgattccag gcagtgccgt
      841 cgagccgcgg ataaatcgtc agtgcggcct gcggacgtcc cgcgagcgtc cagagataga
      901 ggcagccgtc gggttggccg cggaccggat tggtccagcg ctggacgggc tcagcggtgc
      961 ggtcgagctt gaattgattc ttcgcgcccg cggtcagctc gtatcgcccg gccatcgctt
     1021 tcatcgtcgc caggctggct tcgtttgcct ttgccgggtc ctccgcggct ggcggcggtt
     1081 gacccagggc ggcaagttca gaaacgaccg caaagaacgc caaccaggcc aatcgccgag
     1141 agcaggtgcc agtcatttcg accctcccgc tacgcgtaag aatgcgggat ttaggataca
     1201 gacaaaatgt ctatgttgca aacgggccgt tctagcgcca ggcttgtaag gcgataaagt
     1261 ctatctattt ttaccagttt gccccttttc acaggggtga actcggatca aagtggaatc
     1321 tgtggaaggt gctcttaaca caccggtcca gggaggttat cagtccgcct cggatctccc
     1381 cgaggagcgc gccatggacg gcaactctcg taaccggatg ggaatttcgg ccattgcgac
     1441 ctatgagccg ccgtggctgc tcgcgaacga ttggttcgac ggggtgattc cccgcaagtt
     1501 cgtccaccac acgggcatcg aggcccggcc ggtttcgcag gaagatgaag tcacgatggc
     1561 ggtccgcgcg gtcaaaaatc tccagcggga ggttggctgc gatttgagcg cctgtgcggc
     1621 ggtcgtgttt gcctcgcctt cgttcattcc gccggccgcg gcacaccggc tcggcggcga
     1681 gcaagtggcg cggcaggagc ggctgcgggt ggccggccgg caactcgtcc gccgcctggg
     1741 catcgcctct tgccccgtgg cggcgatcaa ctggttctgc agcggctaca gccgggcggt
     1801 cgcgctggcg acgcggcggt tcgcgccgcg gctgaagctt cagccgcatc agtacctgct
     1861 ggtcgtcacg tcgagccgaa tcagccggat cacggattat tcctgcccgc agacttcggc
     1921 cctgttcggc gacatggcga cggtgacgat gctggcgccg ctgtccaatc agcggcatcc
     1981 ggtacacttc gaattgctgt atgccgatgc ccgcaagcaa cccgcggacg gcgtgttctt
     2041 cgaatttcat ttgcggcaga acgtacccgc gccgaccgaa gaaggcgggc gggagatcgc
     2101 tgccgagcgg ctggtgtttt cgctcgacgg catgtcgatc gccgatgccg cgccgcgggc
     2161 gatggccggg gcgctggccg aggcgctgac cgccacgggc ctgcggcccg aagacctcaa
     2221 attcgtcgtg ccgcaccagg caggtgccgg aatcgtgcgc ttgaccgcca tgaagctcga
     2281 ccaattgggc gttcgggcag aggtgatcaa cggcctgacg cagcgggtcg gcaatatcag
     2341 ctccggctcg attccctacg gactgaagca gacctggaac aagctcacgg gcacgatcgc
     2401 ctgtcccgcg gctgccgtcg gctcgcccgg cgtaaaggaa gtttcgcagg gctgcctgct
     2461 cttccgcgcg acgccctacc acgaccagat cgcgctggcg gtggcgtagc tacctcttgg
     2521 gcgcccactt ggccaggaac tcgagcacgg cgcgctcgct gtagcctttg ccctcttcta
     2581 gctcctcggt gttctgcgaa tggagcagcg tgccgtcgga ctcgaggacg aaaatgtgcg
     2641 ggtaaccctt gatcgccgga tacttggaga ggaactcctt gttctggttc tcctggctgt
     2701 agttgacctt ctgaatgacg aagtccttgg ccagggcggc ggcgactttc tcgttttcgt
     2761 gaaagtactt attcatcagg tggcaccagc cgcaccaatc gccgccgacc tgcacgagga
     2821 tccgcttgcc cttggctttc tcgatcgtga gcttcaaatc ggccgcggcg tcgcgcttgg
     2881 gatcgtaggt ctcgaccgaa tagacgggtt tgacggcagc tggcgcggcc ttggcgggga
     2941 catcgtccac cgcactcgcg gtccggccaa gaaggatcaa tccggcgaat agcatcagac
     3001 gggaaacagc tttcatgacg agactcctgg ttttgcttcc gccgccgact cttcgacgat
     3061 ggtacagtcg ggcagcgatt gcagaaagat gcggccgtag ggcttggtat gcaggcgcgg
     3121 atcgagcaac acgaccatcc cgcggtcgcg ctgggtgcgg atcaggcggc caaagccctg
     3181 gcggagcttg atgaccgcct cgggcagctg atagtcgcga aacgggttgc cgccggcggc
     3241 ccggatcgcc tccaggcggg cttcgagcag cggctgatcg ggcacgctga aggggagctt
     3301 gggaatgatg acgttggtca gcgcgtcgcc gggcacatcg actccctgcc agaagctgtc
     3361 ggtgcccaaa agcacgcccc gcggattctg cttgaactgc gccagcagct gcgagcgcgg
     3421 cgtgccttcc gcctggctat agagcgccag gtcttgttgg gcgagccagc gcgacaggcc
     3481 cgcagcggcc cggcggagcg cgtcgtagct ggtgaagagg acgaaggcat ggccgtcggt
     3541 ccgctcgata tatcgctgga tgaccaccag cgaccggcgc tcgaattcct ccttggcggc
     3601 ggtcggatcg ggcaggccgc ggaccaggat cagctgggcc tgctcgcgat agttgaacgg
     3661 gctgcccgcg cggagcgtct ggcagctcgt caggcctact cgggacttga aaaagtcgaa
     3721 ctgcggcggg cggccgacgg ccagggtggc gctggtcatc accacgcact tcgtcttcgc
     3781 gaaaagttcg tcccgcagga ccgggccgac gtcaatcggc gcggccgata gcgccacgcg
     3841 ggtgcggccc gaacggctcg ccgcggtttc gatccaatag accgaatcgg cagcgccctg
     3901 ctcgatccac tgggaaagct cgccggccag cgccgtcagc cggtcgtggg cggaagtgaa
     3961 gtccagccgc tccgattcgt tttcgatctc ggccgccgcg cggcgcagct cgcgagccag
     4021 cttcagcagt gccgggctga gccggttatc gagctgattc gactcgtgaa tccggccggt
     4081 gcgattgccc gggcgctgcg tgagggcggc cgcatcgtcg aacagctcgt cggcccgcgt
     4141 gtggcattcg accgtcaaat cctgcgcctc gtgatacaag ggcgacgcga gcaagccttt
     4201 atgagtgcgg tcgttgaaga gcttgttcag caggaactgc acctggccag aggtgacgcc
     4261 caggcccagg tggtcgccgg cgaccgcttc cagcgtgtgg gcctcgtcga aaatgacggc
     4321 gtcgtagttc ggcaggatgc tcgccccggc ccgccgcaga gccaggtcgc tgaagaacag
     4381 cgcgtgattg acgatcagca gctgcgcatt gacgatccgc cgccgcgcgc ggaaataaaa
     4441 gcacttggcg tacgttccgc atttgcgccc caggcagttg ctgctgtcgc tggccacttc
     4501 atcccacacc gacgggcgcg ggcggaacga aagatcgctc agcgacccgt caccggtttt
     4561 cttggaccag gcgatcacct tgcgcaactc gcgcagctcc tcctcctgcg aaaacaggct
     4621 gacggaacgc tcgccggcca ggttcatccg ccgcaggctg agataattgc cgcggccctt
     4681 gaccagcacc gcggagaatt catcggggat cacgctccgc aaaagcggca ggtccttctg
     4741 cagcagttgt tcctgcaggc tgatcgtgtg cgtggagatg acgatccggc ggcggggccg
     4801 ggtgtcttcg tcgtcagaat cgtcgtcttc tcgcgccggc ggttctgcgg tgacataggc
     4861 aatggccgga gcgagatagg caaagctctt gccgacaccc gttccagcct cggccaccag
     4921 gtgctggccg tcaaagagcg cctgctcgac ggccgcggcc atctccagtt gctgcgggcg
     4981 gtgctcgtaa ttcggcaggc gggcggcgat tctgccgccg gggccaagaa tgtgggcgat
     5041 gtcgatcacg cgcttacgat accgcggcgg ccgtcactgc cgctacaggc cgcaccaggc
     5101 gaacgccgtg agcagtgttc gcaacaaaaa tcatccagat tccctcaagc atgaagtttg
     5161 acagggggcg gaatcgacct agggttgtct cggaagggag cggcggccgc aaggtcgcgg
     5221 ctcctgactg actggtacga ttctcgtctt tcgtcattcc gccgcgttcg cggcttacat
     5281 ctcggatcgc gagaactgct ggtcggcctg ctgccttgca tcctcgaaaa ggcaaaccgg
     5341 ctgtaatggc cggggcgcaa agctataggg tcgctgctgg tgcaacgcca gcggcggcag
     5401 cctggctgcc gaaggggtgg accgcgtctt cggatgcggt tgaccgattc agcgtgcgag
     5461 atgacgaggc gaatgggaag cggagtctag cgcccgccgc gaggcaggcc agcggctagt
     5521 gcgagccgcg gcggcgggcg gactcgaacg gaggcggtgg ccagttgcac caaattggct
     5581 gccggcggct tcggccgcag ccggagcgac tcgttcgctc tcgtggaaca cggcacggtg
     5641 gttggcaaca cggaagagaa agcactggtg gggtccgcac gaccccgtgt ttagctcgtc
     5701 tcgtcacttt ttcagagaga ttgcaatgaa caagtttctc aagtttgccc gtaacttcct
     5761 ggtcaacgaa gatggcccga ccgcggtgga atacgccgtg atgctggccc tcatcatcgt
     5821 cgtgtgcttg acggcgatcc aggcgatcgg caccaacgcc aatgcgacgt tcaacagcgt
     5881 tgccggcgtt ctgcctggcg gtgcttcgta agccagcttg tgctgatttc gcagtttaaa
     5941 caggccaccg caccaaaagt gcggtggctt gttgctgccg ggaccagttt ttcgacctag
     6001 gtttgagaac ccggacagca cgcaaggaga gacgccatga cgcccatcct tcccagcgaa
     6061 ttgctggccg gcagttggga gctgatttgc tgcgcctgca tgggctttgc cgctctgttc
     6121 agctatttgc tggcggcgcg gtagccgctg ctaaacgcag cgccgacgaa cacacacggg
     6181 aagaaagcac ctggtatgga tctcgctact gactatgccg ccgtcattgc ggagcactgg
     6241 accatctggc tggtcagcgt cgtgctgatc gtcgccgcgg tcatcgacgg ctacgagctc
     6301 cgcgtaccga acctgatcac ctacccgttc atcatcgccg gctgggtcta cagcacctat
     6361 gccttcggcc tgcaagggct gggctggagc ctggcgggca ccgccctggg catgagcatc
     6421 ctgatggcct ttcacgcgat cggcgggatg ggcgcaggcg acgtcaagct catggggggc
     6481 gtgggagcct ggatctactg gacgcacacc tggtactgca tcatcggctt cgccctggtc
     6541 ggggcggtga tggcgatcgt gatgatcgta gtgagcggca agtggaagca ccactacaac
     6601 cagttctgga agatcgtcaa cgagatcacg accatcaagg acccggccaa gctgtcggcc
     6661 ctggccgccg agcggaaacc gaacatgatg ctgctgccct atggaattcc gatcgcgatt
     6721 gcgaccatca gctacttttt gtggactggg atgctcgtat gagtaagctt tgcacccctg
     6781 ctatcgccaa tttgctgtca acggtctggc aagctgcgtc ctttggcgca agtctgccgc
     6841 cggcgaaaga caagttggaa cgatcttgcc ggatattccg aagaaaacga cgggccgcgg
     6901 cggcggtgga gtttgccatc gtcgccccgg tgtttttcct gctggtcttc ggaatgatcg
     6961 agtacggacg gatggtcatg gtccagcaag tcctgaccaa cgcctcgcga gaaggagcgc
     7021 gagtcgcagt gctggacggg gccaccgtcg ccacggtcca gacgcgggtc aacgactacc
     7081 tcgccgctgg ctcgattacg ggggcaacga taacggttgc accgaatccg ctcagcggtg
     7141 cggaatttgg cgaccctgtg acggttaccg tctcgattgg cttcaatcaa gtaagctggc
     7201 tgccatcgcc aatgtacctt gggggaaaga ccatgacagc atcgaccgtg atgcgtcgtg
     7261 agacggtgca ataattaacc cctggattcg gcacgccggt tgaaacctga ccgaacactg
     7321 tcccggaaaa caccttcgcc cgcgtttgta aaggaatcga ccgatgcgta tgaaatcact
     7381 gatcctgatt ttcatcgcgc tgggctgcgg cctggtagcc gcggtgggta tcagccaggt
     7441 catgagcggc ggcggctcgg cttctacgct ggagaccgac cagatcctgg tcacgctgac
     7501 cgacatcgac atcggcacca agttcgacgc ccagaacgtc aagctcgaag actggcccaa
     7561 gaacaagatt cccgacggcg cggtccgccg cttcgacgac gtcaaagata aatacgccgc
     7621 ctcgcggttc tacaagggcg aaccgctaca cgccagccgc gtcagcgaca tgctgaacaa
     7681 cccggccgtg aaggttccgc ccggattccg cgcgatgccg gtcaaggtcg aggaagacac
     7741 ggtgctcaag gccatcggcc cgggcgaccg cgtagacgtg atcgtcttcc tccgccgcaa
     7801 tccgccggaa atcatggaga cgggagcctt cccgattctc cgcaacgtcc gcgtgtttgc
     7861 cgtcaacacg aacaccgaac gggccgccga cgccaagggc gaaacgacca acttccgcac
     7921 ggtctcgctc ctggtcaaag aggcccacgc cggcgagctc gtcgtcgccg ccaagatggg
     7981 caacatcttc ctcaccctcc gccgtcccga cgaagtggac tcggccgagg gcgaagaagt
     8041 tacgccgatc aaggaagttc tggctggccc gaaccggctc cagagcgaac ccggttcgcg
     8101 cgggccggcg ccttccacac ccacgacgct cgacttcgtc cgcaacgcga ccgcggctcc
     8161 ggccggtgga cgcgacccgg gcgcccggcc cgagtggtcg atggtgctga tgagcccgag
     8221 cgattggaaa cagttcgatt gggccgacgc caagggcggg ctgcccaccg agagccggct
     8281 cagcgccgct cctccggttt cggcggaacc gctcgacaag acgagcggca gcaagccgga
     8341 aggcagcgac aacgacaacc cgggcacgcc gccgccggac ggccacaaga ccaactagca
     8401 caacgcacac acctgtgccg ggcgaccgtc cggcacacta acgaaacgcc gcagttcgac
     8461 aggtacgaat cgccactcac ggatttttca cggatcggga ttactcgcgt taacccagaa
     8521 cgcaaggaag cacacggatg tctcacccat cgcttaaggt ggccatgctg gccgctctcg
     8581 gttgcgtcct cgcgggcgca atccccgctt tcgcccagca gccggccccg cccggcggcg
     8641 tcaagttcca ggtccgcggc cccgccgagc gcctggagat gaccgtcaat accagccggg
     8701 tcgtcgaatt cccgttcgac gtgccgcgaa tgctggtcaa caatcccgac ctcgtgcgag
     8761 tcgtgccgat ctcgcccaag tcggtgcagc tctccgccgt gaaagccggc atcacccagg
     8821 tcaacatctg ggacatcgac ggcaacgtca cgaccctgga cctgatcatc ctgggcgacg
     8881 tcggcgagtt ggacctgacc ctgaagagcc tgttcccgga ggcctcgctc cgcctgcggc
     8941 cgcttaacag cagcctctac atctcgggct tcgtgcccaa ggcggaaatg gtcggcagcg
     9001 tcatccgcgt ggcgcaggac tatttcccga gcatcatcaa cgacatgacg gtcggcggcg
     9061 tgcacaagat cctgctgcac gtcaaggtca tggaagtctc gcggaccaag ctgcgggccg
     9121 tcggcttcga ctgggacctc gtctccagcg gcggcttcgt gacgcagagc gtctcgcagc
     9181 tcgtgccggg cgtgttcccg atcaacgccg acgccttccc gacggttcgg ttcggcatca
     9241 ccagcggcga ccagttccgc ggcttcctgc agcttttgcg gcagtacgac ctggccaagc
     9301 tgctctccga gccgacgctg accacgctcg acggccgccc ggccagcttc aacgttggcg
     9361 gccagattcc gattccgatc cagcagtcgc tgggcgtgac cacgatccag tggcgcgaat
     9421 tcggcacgcg gatcgacttc gtgccggtcg tgctgggcaa cggcacgatc cgcctggaag
     9481 tccgccccga catctcgcag gtcgatagct cgctccgcga cagcgtgacg ggcaccgtcg
     9541 gcttccgctc gcggcgggcc gacacggcgg tcgagatgaa ggccggccag acgctggcga
     9601 tcgccggcct ggtcgagtct cggacggaag ccaccaaccg cggcattccg atcctggccg
     9661 acctgccctg gctgggtgct cccttccgcc gcgttcagga gcgcaacaac gaaatcgaaa
     9721 cgctgatctt cgtcacgccg gaattctgcg aggcgatgga tccctgtgaa gtcccgccgt
     9781 gcggaccggg ccagctcacc accagtccca cggactgcga gctgtacggc cgcggctaca
     9841 tcgaagtgcc caagaattgc cccacgcccg gccctggctt gatgggcccc agcggctacg
     9901 aagtgctgcc gggcgctggt gctccggtca atggcggtgc tccgctggct cccccgattc
     9961 cgccgcccag ccccagcgcc ggcacaaccg gcacatcccg ccgggctctg gcagtcggca
    10021 caggcgctgc cggccgaccg gccagcagcg ggcgggtgtc ggacctgccc agcagcagcg
    10081 gcacttcgta ttattcgggc gctgggacgc aaagtccggc cccggtcgtc aagccggcgt
    10141 tgatcgggcc ggctggatat gacgatctaa ggtaaacggg cgccgaccgc ggcgctcgta
    10201 aactgcgacc tacagttaaa gggcaggaag ccacctcctg ccctttactt tttaccgcgg
    10261 agcccccgct ccgcccgccc ctcccgcctg acgaatcact cgctgcgatg agcaacgtcc
    10321 ttcgcctggc catcgtcgat cccaacgaca acaaccgcac gcagctcaag aacatgctgc
    10381 tggggatgga catcgtgtgg ctcgaggcgg agtgctcgcg gtacgagttc ttctccgacg
    10441 tgatcgggca gactaagccc gacatcggcg tggtggccct cgacaacaac cccgagaagg
    10501 ccctcaagct catcgagcag atccgcgaca tcgcgcccga ttgctcgctg cttgtggtca
    10561 gcagctcgac cgacgggcaa gtgatcctca aggccatgcg gagcggggcc aaggaattcc
    10621 tcacgcagcc gatcaaggtc gaagacctga tcggggccct cggccggatc agctcggcga
    10681 agttcggcgc cggcgacggc cgcaaccgga cctgccacgt gatcaccgtt tgcggcgcga
    10741 ccggcggcgt cggcgcgacg agcctggcgg tgaacctggg cagcatcctg gccaaggacg
    10801 agaagaacag cgtcgtgctg gtcgatctgg acctggccct cggcgacgcc gacgtgttcc
    10861 tcgacacgat ccccgactac acgctggtgg acgtcgccca gaacatcgcg cggctcgact
    10921 tcacgctgct caagcgctcg ctcaccaagc acagctcggg cctgtacctg ctgccgcggc
    10981 cggtgcagct ccaggactct tcgcaaatca ccgccgacga cctgacccgc gtgctgggcc
    11041 tgctcaaggc ctcgttcacg catatgatca tcgatctgtc gaagggctat acgcccatcg
    11101 acaacgtggc cctcaaatcg gccagggaca tcctgctggt cacccagctc gacctgccct
    11161 gcctgcggaa cgtcgtgcgg ctgatgctct cgttcaacga agacgaggcg atcaaggaca
    11221 aggtcaagat cgtgatcaac cgcgtcggcc tggacaacgg gtcgatcagc ctgaagaagg
    11281 cccaggagac gatcggccgc gagatcttct ggcagttgcc gaacgacttc aagacgatgg
    11341 tcgagatccg caacaacggc gtgccgctga tcgagcaggc cccgcgggcc ggcattacgc
    11401 aggcccttgt cgccctggcc gagcgcctcg tcggcgaggg ccgctccaag gacgaggccg
    11461 acagcaacaa gggcgccaag aacaccagca cgggctggct gaacttcctc ggcggcaaga
    11521 agaagtagca gccgtcaagc gccgtatttg ctcagccggc cggcgttggc caaagccagc
    11581 gtcatcaggt acttggcttc gaactggccc aggccgccgc gaacggcggt gttctcgcca
    11641 aaggtcgcgt ggccgtccgt ccggcagcag ttgctgacga gcaaggtcgg cacgggatgc
    11701 cagctgtggc tcttgaggaa gctcggcgtg ctgtggtcgc cggtgacgat caggaccgtc
    11761 ggtttcaaag cagtaattcg cggcacgatc gcgtcgagct cttcgatccg cttcaccttg
    11821 gcgtcaaaat tgccgtcctc gccggtgctg tcggtgtatt tgaagtggat gaagaagaag
    11881 tcgtacttgc tccagttggc cgccagcgtg tcgatctgct cgctcagcgt ctgtgatttg
    11941 ccgacgatct ccatgcccac gaggcgggcc aggcccttgt acatcggata gacggcgatc
    12001 gccgcggcct tgaggccata aacctcttcg tagctcggca gattcgggcg ggctgcaaaa
    12061 ccgcggagcg tcaggccatt ggccttcttc tcgccggcca gcagcttcgt cgccgcggcg
    12121 atccactggt tggcaatttc ggccgtcttg cggctcgcat cgtcctgggc cgcggccgcc
    12181 agcggcggca cccccgtcgc ctgcggatcg gtgtccttca cgtttcccgc cagtccaggt
    12241 ccacgaaaga ccacgacgaa gcgatgctcc ttcaccggct cgacgaacgt ctcatagccg
    12301 ggtgtcttca cctcgcgcag ccgcaccgcc agcggggagc tctcctcggt ggggatgcgg
    12361 ccggcgcggc ggtcgctgat cttgcccgcg gcatcgagcg tgcaaaagtt gccacgggcc
    12421 gctacatcgc caggctgcat gtgaaagccg atgcccgtgg cctccagggc gccgcggccg
    12481 atcaggtact tgatcgggtc gtagccgaat aggcccaaat ggcccggccc gctgcccggg
    12541 gcgatgccgg gaaaaacggg gatgctgccg ccctgcacgc cgatcttggc cagcgcgtcg
    12601 aggttgggct tgcgcgctgt ttccagctcg gtcttgccgc cgggcgtcat cggcagccct
    12661 cccaagccgt cggcgacgag cagcacgatc ttggaatcgt ttttggtgtg gagctcgcgg
    12721 gtgagaaggt gggggtccat ggcagaggct agggctgggg gctagggctg gggagtgcat
    12781 ccattagctg cgcagcgtat tcgccgatac ctgtgaggac gatttctcga ggtttcgtgg
    12841 cggcctcggc aatccggcgc acgatggcgg agccgacgat caagccgtcg gcgaccgggg
    12901 caaggagctt tacgtgagcc ggctggctga tgccgaagcc gatgcagagc ggcaagtcgg
    12961 tctgcgactt cagccagccg acgttctcca cgagcgacgg cggcagttcg gttcgctcgc
    13021 cggtgatgcc cgcgatcgag acgtaataca agaagccggt gctggcccgg gcgatttgca
    13081 gggcccgctc gcggggcgtg gtcggcgtga ctagctggat caggctgaaa tcggccgcct
    13141 tgcagatcgc cgccagctcg cccgcttctt ccacgagcaa gtccgggacg atcgcgccgc
    13201 tcgcaccggc ccgctgcgcg ccctcgacgt atttcttgag tccgtaccga tggacgatcg
    13261 cgtaactgac catcgtcacc agcggcatct tcacctgccc gctcacccca cggagcatct
    13321 cgaagatcgc cggcagcttg atcttcttcg ccaaagcccg cgtgtacgag gcctggatca
    13381 ccgggccgtc ggcgatcgga tcgctgtaag gaattcccac ttcgcagagc gtggccccgc
    13441 agcggtccaa ttcgcggatc acgtcggccg tgaacgccag gtcggggtcg ccagccgtca
    13501 cgaacggcat gaaagctttg cggccggatt tgcgaagctc ggcgaagagg gcgtcgatgc
    13561 gggacatgtt gacttcaccc gctcctcagc cgcgcaatct ccgcggcgtc tttgtcgccg
    13621 cggccggaga ggcagacgac gatcgactcg cctttgcgca tctgccgcgc ctgctccatg
    13681 gcgcgaaaga tcgcgtgcga gctctccagc gcgggcaaga tgccttcgtt ccgcgccaat
    13741 gcatcgaaac cggccaacgc gtccttatcc tcgcaaaacg tgtaagtcac gcggccgatg
    13801 tccttccaat agctgtgctc cggtccgacg ccgggatagt ccaggccggc tgaaatcgag
    13861 tgcacgtcgc aggtctggcc gtcttcgtcc tgcatcacgt agctgtagct gccgtgcagt
    13921 acgcccggcg agccgtacga cagcggcgag gcgtggtcgc cgggctgggg gccccggccg
    13981 ccggcctcga cgccgagcag ccgcacgctc tgatcgccga tgaacggata gaacatgccg
    14041 gccgcattgc tgccgccgcc gacgcaggcc acgaccaggt cgggcagttt gccgattcgc
    14101 tcgagcgact ggcggcgggc ctcgcggccg atcaccgctt gaaaatcgcg gacgatccgc
    14161 ggaaagggat gcggcccgat caccgagccg atgatgtaat gcgtggtctc gacgctggcc
    14221 atccagtcgc ggagggcttc gttcaccgcg tcgcggagcg tccgcgagcc gctgctcacc
    14281 ggccgcactt ccgcgcccag cagcttcatg ctgttgacgt tcggcgcctg gcggcggatg
    14341 tcctcctcgc ccatgtacac gacgcagtcg aggccgaagt gggcgcaggc cgtcgcggtc
    14401 gccacgccgt gctggcccgc gccggtctcg gcaatgacgc ggcgcttctt catccgcagc
    14461 gtcagcaggg cctggcccag cgtgttgttg atcttgtgcg cgccggtgtg gttcaggtcc
    14521 tcgcgcttca gccagatctg cgccccgccg cactcggcgg tcaggcggcg ggcgaaatag
    14581 agcggcgatg gccggccgac gtaatgctcg aatagctcgt ccaggtcggc ctgaaacctc
    14641 gcgtccagcc gggccttgtt gtactcggcc gtcagctcgt cgagggcccg ggtcagcgtc
    14701 tcgggcacgt acctgccgcc gaacgcgccg aatcgcccgg agctgtcggg aacggcgttg
    14761 gcagtaggaa cagtggcggg cgcggtcatg cagtgccgga tttgcgggga atcttcgatt
    14821 gtcagtgggc tttcccacat ggtcaaggcc ggcgggcgcg tcgctaccgc gctcgaatca
    14881 agcatgcttc ctgctcgccg ctggtgaaca gggccatccg cagatcgacg tgcgagagcg
    14941 accaccctgc cagctccttc accggtgcgc cgagctcgtc gacaatcggc tggtccttct
    15001 cggcaaacag ccggcggccc tgaaagcgga acagcgcgcc cggcggcgaa ggcatgtagc
    15061 ccaatagctc cagctgcttt tccagcgcgg aaacgggtgg ctcgcccagc ggcagcggct
    15121 tgccgttaac catctgcacg ttctgctggt tgagggcccg gttcactttg caattgacga
    15181 aggtcacgtt ctcggccagc gacacctcga tccccaaagg agcgcagatg atcgtccccc
    15241 gggcgatcgt gatctcgccg tgcccgcggc tgatcaggat gctgctggtc gggttagcgt
    15301 tgacgacgcg gtccgaggag cagcggaagt agcgcccggc gatgatcagc gaattctcga
    15361 ccgagctcgt cgtggccacg ctgcgggcga tcacgatgct gttcttgacg gtcgaaagct
    15421 cggcgttgcc gtcggccagc acgatcgaat cgtagacgct ggactgcttg acgtgcttgc
    15481 cgatcttgag gtgcccgcgg agcagctggt cggtgtcgcc agccggtttg acgtccttga
    15541 ggcggaccgg ggccttcagc tccttgccgg tggcctcgcc cagctgcttg agcagcttgt
    15601 cgatccagcc ttcgatggcc gcgtcggtcc agttccgctc cttccaggcc gccccgccgg
    15661 cgtgcgtgtg aatgcggtcc agcaccagtt gcagcgattc ctgcggcggc ggatcgcggt
    15721 cgatgtcgcc cgcccaggcg gccgccgcga agagcgtgcc gcacaggcag gcggcgatca
    15781 tcgagtgcgg gctcatagtt gtcctccgcg ggcgggcagc agatgatgat aagctaagct
    15841 tcgccccaga cacaaacacg cgtccgtatt catgaccgac tcacggcccg ctaaagcgcg
    15901 gctgggctgc ggctttctgc tcgtctcggc cgcgctaacg tgcgtgatgc tggcgatcaa
    15961 cgggctgatc gtgacgaacc ttgttgccgc ctattcgccc ggcccgctgc cgctggaaaa
    16021 ccagcgggtg gcgcaggccg ccgtgttcct cggccctgtc gtgctgctgt tcgtcgagtg
    16081 gtggattttc gatgtggccc tggactggct ccgcccgcag cgtgcggccc agggccggcc
    16141 gaaagcctga ctcgaaagga aattcggcat gcagcctgtg cgttgcttgt tgacggcact
    16201 cgtggcgctc gttggcagtg cggcgctggc cgccgagccg gcggcgcggc ccaacgtcgt
    16261 cttcatcctc accgatgacc agcggtggga cacgatcagc ctgcatccga agtcggtcgt
    16321 caagacgccc cacatcgatc ggctggggaa agagggcgtc tattttaaga gcagcttttg
    16381 cacgacctcg ctctgctcgc ccagccgggc gtcgatcctc agcggcttat atgcccacac
    16441 gcacggcgtg acgaacaact tcaccgagta tcccaatgac ctgccgagct ttcccaaggc
    16501 cctgcaggcc gccggctatg ccaccgccta catcggcaag taccacatgg gcgaagagaa
    16561 cgattcgccg cggccgggct tcgactattt cgtcacgcat aagggccagg gcaactactt
    16621 tgacaccgaa ttcaatgtgg ccggcgagcg gaaagtgctg cccggctatt acacgcacgt
    16681 cgtcaccgac ctcgccgaaa aatggctccg cagtgtttct ggcggcgggc ttcgcggcgc
    16741 cgcgggcgac aagccgttcc tgctgatgct cggccacaaa gcgccccaca gcttctactt
    16801 cccggagccg aaatacgaac acgccttcga tggcgagaag atcgagtatc cgaggagcgc
    16861 gtttcagctc gaggacaagc ccaaatggtt cctggcccgg ctcgatacgt ggcacgggat
    16921 ctatgggccg ctcttcgaat accgcaagca gttccccgac cggcgcccgg aggcggtgaa
    16981 ggattttgcg gccatgaccc gcggctattg gagcaccatc ctctcggtcg atgacagcgt
    17041 cggccggctg tatgctgtgc tgaaggagct gggcgagctc gacaacacgc tgttcatctt
    17101 cacgggcgac aacggcctct tgaacggcga gcacggcatg gtcgataagc ggaccatgca
    17161 cgagccgagc atccgcacgc cgctcgtcgt gcggctgcca aacacactaa cccgacgcgc
    17221 cagcgaggga gtgccgcgcg ttatcgacga acaagttttg accatcgact tcgcgccgac
    17281 catcctcgac atctgcggcg tggccccgct ggacaagacc cacggccgct cctggaagca
    17341 gctcgctcaa ggcgacgccg ccggctggcg caaaagctgg tactacgagt acaactacga
    17401 aaaacagttt ccttacacgc ccaacgtccg cggcgtgcgg accgatcgct ggaagctgat
    17461 ccgctatccg cacggcgacg gcggaccgga ccggcacctg gcggagctgt acgacctgca
    17521 ggccgacccg gaagagacgg tcaatctggt tgcgaagccc gagctggccg ccagcgtggc
    17581 cgagctgaac gcggagctga cgcgcctcat ggaagccgcc ggcggcctgc ccgacaagat
    17641 gccgctggac gagggcatca agagcggctt gcccgagaag gcgattcgct gactctcgtc
    17701 ctgctctgcc gagcggctgt cgggcaattt actgccagac tttggactgg acgctcgtgg
    17761 caacttattc ggcggctggc cgcgctgcgt tcaactcgca agtcattgcc gcaccgctgg
    17821 ttggggtttt tggctcggtc tccaaagcgg cgaatttgtt gccaaaaacg gcgctcatgg
    17881 gggtttgtgg caatttattt ggcaatcaat tcggtttagc ggggtttcgc gcgacccgaa
    17941 tcgtgggaaa gtccgttgtc catgatgctg cacccggacg aagcgcgaca aaaacaactc
    18001 gtgcgagaca cgggccgagc acgaaacact aagcacacga agactcagct cattccattc
    18061 gtggccttcg tttctttgtg cttaacttgc tcggaccttc cgcaatttga cacaattgtc
    18121 cacacccgtt tgcggctgtc aatatacttg tacacatttt gttcattgga tgccaacttg
    18181 cgtcaaatgc ccgccgcgca agtccaaggc cagccagact ttccgctacg acgccagcgc
    18241 ctaacttttt ttcgacgctg ccggcgaaat ggactagtgc ggtccattcc gccgcgcatg
    18301 ggctacactt tctagagggc gatcccatga tccgccgttt agctcaaccg ctgggagtgc
    18361 tggccctggc cgcgtgcgtc ctgctgacgg ccctgggcgg gctgtcgcat tggcgcggaa
    18421 tcctgatggg ccgaatcggg ccacagcaca ccgtgctgat ccgctcgacc tacggcaagg
    18481 cccaaatcga catccaccgc accgacgccc gcgagccaac gctgtggatc tggtccacct
    18541 ggccgcacga attcgccttc cgtgagccga acctcggcgg ctggtggcgg cacggcggct
    18601 tcggcctggc ccaaggtggc acgcgcggcg gcaacactgt gcagcagatt acccttcctt
    18661 actggtcgct cacgctgatc agcctgctgc tggccagcgg gttgttgctc gccggtccga
    18721 aacaggctca gcagcagggt gtccgggatt ggaaacaatc cgaattcgcc gaccattgca
    18781 atgctgcatc gacggcatca tcactcgacg tcgagctggt gaaacgcacc gagacgaagt
    18841 tgagtcggaa gctgccgctg gggtacgtcg ccaggatgtg cggcgacaac ggcggagctg
    18901 tcgccgcggc aagagataaa tggtctttgt acccgatctt cgatgacagc gacaaaaagc
    18961 gcctcgagcg aacttacaac gacatcgtcc gcgagaccgc cgaagctcgc cagtgccccg
    19021 aattcccccc ggatgccgta gccatcggcc acaatagcgg cggcgacaag ctggttttca
    19081 tggccgacgc cgaagccggt cgctacgcgg acgcagtcta ccggtgggac cacaagaccg
    19141 gcaagctgaa gcaagtcgcc gctgaattcg agcagttgaa gccggcttag ggattaggca
    19201 tcgctcgcgg tgcgatagac cggcttgaat ctcctgcggg gcgggcaatg tagggagtcc
    19261 gctttgagct gcccgagcaa caaataaccc tcgtggttgc gatgctcacc gacgaccaac
    19321 gcaaggtatg ggaggcctat gaagcggcgg agcgtcgcgg cccgcgcgcg gaaatgctgc
    19381 aatcccttaa ggcgtttctt gattcccttg cgacttcgcc gccggccgac tggtttccgt
    19441 gggcgagatc gcttgccgag caggttgtag accacggcct ggacttctac atccgccagc
    19501 cgctgtttga gcgggctgtg tttcctgcgc tgctggccgg ctatcaggca cgtctgccag
    19561 gaagcgcccg ctggctcgcc ggcctatgcc agaacatata tcgatctccg gcatgccggc
    19621 agcagcttcc accggaagag tccacagagc tgggcatgct gtgggcagcg cttcggcatg
    19681 atccgggtga tcggcgcgct cgtgtccgtc tgatcaagag cattgctgac cggttgcgct
    19741 attccctcca cgaacttccg gcgggcgtcc tttacggcat ggacggcgca tcgccagaac
    19801 aatgcctgga gcttgaacgt gagctgcaag agttctgtgg cctcgtcgca caggaggata
    19861 tgcatgagac ctacgcgaat ctgattcgcg actgcggctt tcactttcgc ggctatcggg
    19921 actatctctt gcaccgggag aggtaccaga attacggcga gtacatcgcg cacaacgacg
    19981 agatggctgg gggctagcgc tggcgggcgg cttcaactta ttcgttcccc agcattcgat
    20041 tgcgggaggt gaggtgtgga agcgagtttg gctatacata ccgccgctcg tcggtactgc
    20101 ctggagcgat atgcgtgctg ggcggagcgc tatgccgaga tcgtccgcca cggacgagat
    20161 cgcgggcacg accgctacca ttacacctct gaagcgctgg ccacgtttcc ccgttacaac
    20221 gtcctcaatg ccatcagggt tgaacttgaa cgaatcgacg ctggtgagct gagtgacttc
    20281 gaggctgtca aatcgcaagt gctccgcgcg ggtgaaacgg cgaacgatga attcacgctg
    20341 aagcctatcg gggcgatcga ccagcgcgcg accgaagagg agcgcgcggc gttttgcagt
    20401 tacgtcgcaa ggctgtcacc tgcggatctg tatggagtcg agccgctccc atttcgccgt
    20461 gtcctggcag cggatgaatc ggagtcgctt tggtcgcggc ttcggaagcg ctgggggatc
    20521 ggtgcaggtt gctggtatcc gctaggagag tgcacgctgc ccggcgtgga ggccttcgac
    20581 gcctctgcat tcgacaaagc cgcgccgcca gaatgcctgc gaggcatctt gtcggatcgc
    20641 aggatcgagc gcgtctggga gctgcgagaa ttcgggccgg agtacgaact ggccgtcgcg
    20701 ctgtttgatc cgcattacga cggggccgaa gggtactggt cgtcgggcga aatggactgg
    20761 atcgtgtacg cctcgcacga gcgttccgta accgttggtg gatggctgct aaaagaagtc
    20821 atgtcgcttt ggccagcgtg gcaggcacac ctctggcgcg gcgaccgaac ttaacgctgc
    20881 agcggaccgg tcagtaagcg ttggtccgca gctactgcct ctccaccctc agcagcgaaa
    20941 aatcctcggg ctggcgggcg aggtagaggt ggtcgaagag ggcggcctgg ccgtcgacgg
    21001 gcgagcaggc cggtgagcgt gaactcgccg aagtcaccgt agggtgggtc aagcgagtcc
    21061 gcgagcgcag acccacgcgg aagttggcat tcaccaggtg ggtcatcggc cgcggcggcc
    21121 tcgacccacc ctacgaaatt ccccaagccc atcacaacta ctgcttctcc accttcagca
    21181 gcgaaaagtc ctccggctgc cgggcgaggt agaggtggtc gaagagggcg gcctggccat
    21241 cgacgggcga gaaggcgagg ccggtgaggg tgaactcgcc aaagtcggca aacaggtcgc
    21301 gggtgacgac gacgaaccgc ggggcgggca acttctcgtc gatcgtcagc gacgcgccgt
    21361 agcactcgcc gccggggccg gcgtggtagc ggaacttcgc cccttcgcgg ccggtcccgc
    21421 cgggccccca ggtgccgtcg tggttgagct gcaggcagat cgaattgccg ccggccttct
    21481 tccaggcgaa gcggatgtag cggtactcgc ccgggccggg gttctcgcga atcttcatgc
    21541 ccaggttcgg caggttcggg ttgaacttct gatcgcccgt gatgcggagg ctgtacgtgc
    21601 cgctgtactt gtcgcgctgg tcgggaatcg cccggccctc gccctcggtc aagagcgctg
    21661 cgaacttctc gtcgtcctcg aaaatctcct tgggcggctc cgcggccggc ttttcgcccg
    21721 ccaccacatt gatcgccacg tccagcgtgt tgctccgcac cgaggtcgcg accaccggcg
    21781 cggcgatccc caccagcttg gcccccttca gctcgccggc cttgctgccg aaggcgaacg
    21841 tcagcggcag ctcgaactcg ctcttgtccg ccggcacgat gacctgcggc gcggaatagc
    21901 ccttgggcag cccgtcgagc gtcaccacga cggactgcgt gtagcccggc gaccgctcga
    21961 tcttgccgac cagcttgccc ggctcccccg cgcccgcctt gccctcggca gcattcggcc
    22021 cggtcagcgc cagcgtaaac ggcgcgaccg gcgacagcgt ccgcaccggc gcggcgatcg
    22081 aagccacgac agtcttgccg tcggcggaaa gcagctccgc gaccagcacc aggtcccacg
    22141 gctgccgcgg caggtcgccc ggcaccagaa tctgggccgt cacgtccttt tggtcgtcgg
    22201 caaacgtcgc gtccccttcc agccgcagcg cgcggtcgag gtcatcgacg accttgtcct
    22261 ggttattctc cttcacggtc ttcttgggcg tcggctgcgt ggtcagcagc ttcagccgcg
    22321 tcttgccctt cgtgccttcg gcgcgggtca attgcacgcg ggccggcagc ttgccgccca
    22381 gaaagagctg atcgttgtcc cccggcagcc agacaaggcc cagcggcgcg ggctgcgtaa
    22441 tcgccaggcc cagctcggtc ttcactcgcg gctgatactt cgcgccgggg aagtcaccaa
    22501 aggtcgcagc ccggcggacg ggcggcgttt ggctggccga ctgcccaatc agccgcgaaa
    22561 gcgtggcctg cggtgagcct ggcgcggcgc tcagcgtcag caggccgatg gtcgcaccgg
    22621 ccggaatgac gctcccctgc aactgcagct ccgccggctg cccttccagc gacagcgcga
    22681 tggggccggc gtagttcgtg cgcgtgacct ggatgggaat gacctgagtg ccgccggagg
    22741 ggacgttgat ccggtcggtg gccagcgaga gcgagaaatc gggccggctc gtatcggccg
    22801 ccaccatgcg atagacgaag tcgctgccgc cgcggccgag caggtccttg atcgccagca
    22861 cgatgcgatt gacgcccgcg ggcaccgtgt actcgaccat cgggtcgctg ctgcccggcc
    22921 ggtcgtcgcc cgaggcgagc gagccgcctt gctcgttgcg aatcgacagc accgcatcga
    22981 gcggcgagcc aaaccgccgc gccgtcacct cgacgcgcag cttctggccc ggcgtcacgt
    23041 tgagcaggaa cctgtcttcc tcgttggccg ccgacaacag gccgctgatc gcgacgggcg
    23101 cggccggcaa ctcctggagc tggccgcccg cggcaggatt ctcgaccagc tcgggaaaat
    23161 cgctgacggc gattcgcggg gccgcgccgc tatatcgttc agccgccggc agcagcgatg
    23221 ctgtttcgcc gggtgccggg ttagcggtca ggtccaactc gacgacgtcc ggaatgttcg
    23281 acgaggcaaa ccgcacggcc tgcttgctgc ccgtcgtcgc ccccagcggc agggcgaggt
    23341 cggcgaactg caggtcgccg atcttcaggc ggaaatatcc cgggccggca ggccggtaga
    23401 gctggtcgtg caattcgacg gtgtactgcg catcggcggc cagcagggcc tcgacacggg
    23461 cgtcgccgcc aatgatcgcc cgcggcgggc tccaggcgat ctgcgtcccg cgggcgtcgt
    23521 acagccgcag caccggcttc agccccgcgc ccagccgctg ggcctcgacg tccagcacca
    23581 gccgctggcc ctttttgccg gccagcgtca tcttcaggac ctgcgccgcg ccgacggcgc
    23641 cgtgcagggc gatgggtaaa gtcttgggct cggtctcgaa ggcgacttgc ggcaagcgat
    23701 cgacgccgat aacgatcgga ttcgagatgc cgccgctgcc ggccacacgg atcgggtaca
    23761 ggccgggcga cgttgcgggg tcgagcgtga cttcgatctc gacgcggtcg ctcttcgctc
    23821 ccggcttaat cgtctggccg gcgattttgc cggcgatcag cacgcgggcg tcggcgggca
    23881 agtcgctgcc ggtgatgagc agcgtcgtcg gctggccgat ctgcagcccg cgcgggctgg
    23941 cgctggcgat gttgggcgcg gcgctcgccg ggccggcaag cgcataggct gagacgagaa
    24001 ttgtgagggc cagcgggcgg agcatggcat ctccttcaaa cgttggaccg ccctgaagct
    24061 gcgatcagcc gcagggcggt cgagtttcat tcttcgtgcc gcggcaggcg ggcgatgggc
    24121 cgcggcaggc gggatcggtc aatggtctta cggagcaggc gcgggcgcgg gagccggcgc
    24181 tgcgggtttg ggcggctcgg caaacgggtc atccgccgga gcagccggcg ctggagccgg
    24241 cgcgggcgct ggagcagcgg gagccttcgg cttcggcggc tccgcaaacg gatcgtcggc
    24301 tggcggtgca ggagcgggcg cgggcgctgg cgctggtgcc ggcgcgggag ccggagcagg
    24361 ggcagccgga gcgggcgctg gagcaggggc cggcgctggc ttcggcggct cagcgaacgg
    24421 gtcatcggcc ggagccgcgg gcttcggcgc gggcgctgca ggagctggcg ctggagtcgc
    24481 tggcttgggc ggctcggcga acgggtcagc agccggtgct gcaggtgccg gcgcgggggc
    24541 cggtgctggt gcggcagccg gcgctggagg tgtaccaaac ggatcggccg gcgcgggtgc
    24601 aggcgcggga gccggggcag gcgctggcgg tgttccaaat ggatccgctg gtgcaggtgc
    24661 tggagccggc gccggtgccg gtgcaggcgc aggaggcgct ccaaatggat ccgcagcagg
    24721 cgccggtgcg ggcgctggag ctggtgctgg cgcgggtgct ggtgctggcg cgggagccgg
    24781 cggagttcca aacggatcgg ctggcgctgg agccggcgcg ggtgcagggg ccggcgctgg
    24841 cggtgtacca aacgggtcag caggggcggg cgccggagcc ggagcaggcg cgggtgccgg
    24901 ggcaggcgct ggcgcaggag ttccaaacgg atccgcggcg ggcgccggag cgggcgctgg
    24961 ggccggtgcc ggggtggggg ccggggccgg tgctggagtt cctaatgggt cagcggccgg
    25021 tgctggagcc ggagctgggg caggcgcggg cgccggagca ggtgccggag ccggcacagg
    25081 tgtaccaaac gggtccgcgg ccggaatggc cggcgcggg
//
LOCUS       DASRJB010000163         8286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2867839, whole genome shotgun sequence.
ACCESSION   DASRJB010000163 DASRJB010000000
VERSION     DASRJB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867839"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..344
                     /locus_tag="VFB80_04660"
     CDS             132..344
                     /locus_tag="VFB80_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33083.1"
                     /translation="MRRKSRRRENIAVCAHQAEAASRGRMGAGEVVVGWEATWKIRTG
                     SLASSGKVRPQRGHWAGFPGLETTLR"
     gene            582..1949
                     /locus_tag="VFB80_04665"
     CDS             582..1949
                     /locus_tag="VFB80_04665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN33084.1"
                     /translation="MQLAILAAVLAALAAAESGGGPVEGLGWRLLAIAAVTLVAPLAA
                     FLGGQRLALDVAALDRSERSIARLQSIVVGLWMSAVLIVLFIAQWPRIVRGNWQLAGW
                     PLLDELAILTPVIAPLLLVWAGLYRLDRAAQVAAFRARELEPPPAQLGRYLWLHVRHH
                     LGLVLLPALAVVGAFELLGMFNLTRARPDAAWWFALPLLATMLLLMPLAVRRVWRTTP
                     LPVGPLRDELEAICRRERCLVREILVWHTDGYMANAAVVGLSRWLRYVLLTDALLARL
                     KTAEIVAVLRHELAHLQRWHLPLRLALLALPLAWWLAINQVWPHLDAALHYAMVAAGW
                     PAPLVTAVMLPVCMLAYAVIVVGWYSRLLEHEADLAACLADDGQFDSLRAADFGRALV
                     LLIGRSRESRLGQWLHPSLPDRLRFLRGAMERASRCAFERRLVWIKLSIAAGYLAALA
                     VALLG"
     gene            complement(1952..2488)
                     /locus_tag="VFB80_04670"
     CDS             complement(1952..2488)
                     /locus_tag="VFB80_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZN33085.1"
                     /translation="MPADRPLAGRRILVFVGDIYEDLELWYPKLRLIEAGADVVVAGE
                     RSRHKYAGKNGYPCVSDAAIAEQRAKDFDGVVVPGGFMPDKLRRENDVLQLVRDFAAG
                     GKLVAAICHGGWIPISAGVYRGVRVTGSPGIKDDLINAGALWEDASVVIDRHFVSSRK
                     PDDLPDFCRGILQVLAHS"
     gene            2569..3327
                     /locus_tag="VFB80_04675"
     CDS             2569..3327
                     /locus_tag="VFB80_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HZN33086.1"
                     /translation="MSSESSPASESPAGPKWQPLTARQRRVFGVLVEKAKTTPDAYPM
                     TINGLIAGCNQKSNREPQMNLSAEDVEQTLDELRGLGAVTEVQGSGRVAKYRHHAYEW
                     LGVEKTELAVMTELLLRGEQTLGELRGRAARMEPIADLATLKPVVDSLVKRGLMVELT
                     APGRGQVVSHHLYKERELAEIKTQFANYVPPAGSSDDPPAIRERPAPAAAPAVPTADQ
                     LAELTVEVAELRAEVLRLRDQVQGLESKLQAVMT"
     gene            3350..4024
                     /locus_tag="VFB80_04680"
     CDS             3350..4024
                     /locus_tag="VFB80_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN33087.1"
                     /translation="MPELIVEHVSKSFPTRGEPLVVLRDASLTLAAGQNAAILGPSGS
                     GKSTLLFIIGTLDSPSTGSVRLNGTDPFSMREAELADFRNCNIGFVFQDHYLLPQLTV
                     LENVLVPALAAGTPPPATVERARDLLARVGLAERLEHRPAELSGGERQRVGVARALLL
                     KPALLLADEPTGNLDRKSAAAIGELLAELPRQEQAMLLVVTHSLELAQRFERRLELDD
                     GRLRDA"
     gene            4026..7553
                     /locus_tag="VFB80_04685"
     CDS             4026..7553
                     /locus_tag="VFB80_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN33088.1"
                     /translation="MTLLRVITGSLRYHWRIHVAVALGVLAATAVLTGALAVGDSVRY
                     SLRRLALDRLGRIDEVLVVDRFLRQELAGELASQPKFKEHFASATPAILFPSATAESA
                     GEKRRLAANVQVLGVEPAFWDLSVGHSRPKQSPADGEVILNAPLASELNVKVGDSLVL
                     RFGKADQVPADSPLGRRSGQVASLPELTVSEIIPAEGLGRFSLQPSQLAPRSAFVSLA
                     AMQGALDVAGKANTIFVGGLNADVPAPPAASGELAQLLTPRLTDDGLALKHVKMTFGE
                     GQAARTIYEYYSLTSDRMLLDTATERAVQRAFAAERPQPLLTYLANSIQKLTDGAADD
                     KARIPYSTITAIDPAPGGPLVDADGKPLPPLADDEIVLTSWAAEDQQAKVGDKIRVTY
                     FDPETVHGAERESSAEFRVAAIVPLTEPASPFTRRTPAVYEQPPTPANDPDLTPEVKG
                     ITDQASIANWDPPFPFERGRIRPQDDEYWENHRTTPKAYVSLRTGQRLWGSRFGSVTS
                     FRIPANSGVSVESLEKQFLAEVGQSRERLGLDFNPIKRQSLVASAGTTPFDVLFLLLS
                     MFIIAAALMLVWLLFRLGVEQRASEIGLLLALGWSRPQVRRLLLLEGSVVAAIGAALG
                     VLGGIGYAWLMIVGLRTWWVGAIASPFLLLHVTLLSATVGFAAGLIVSLVTIWFSLGR
                     VKNAVVRSLLAGEIVPSAKNESPALRTPRSAFRIQAAAAVLFLAAVGLAFYAATLGGE
                     AQAGAFMGAGAALLTALLLFTVAQLRSAGGRSTLIGGAALARLAFRNASRNVGRSAAT
                     IALMAFAAFLIVAVSAFRMSPTDTGVGGFDLLAQSSQPIYEDLNSDEGRRQLLAGKTA
                     NVAGCTILSLRLKAGDDASCRNLYQPSQPQILGVTPQVIEYFNDSKLPARFAWSASAA
                     KSEAEKANPWRLLATPTPPKEPVPVVLDQNTAMYSLRLYRGIGETFDVTYDDGTKVTF
                     RVAGLLANSVLQGSLLIGEADFERLFPQISGYRYFLIRTPAGKAADVAGVLEDQLSDQ
                     GFDATSARERLTDLLAVQNTYISTFQSLGALGLVLGTFGLAAVQLRSVFERRKELALL
                     RAAGFRRRRLGQMVLLENLLLLLGGLLLGTIAALVAVLPQMLFGGARVPLIDLALMLG
                     VVLVVGISTGLIAVRATLHAPLVGALRGE"
     gene            complement(7557..>8286)
                     /locus_tag="VFB80_04690"
     CDS             complement(7557..>8286)
                     /locus_tag="VFB80_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HZN33089.1"
                     /translation="AVRTFGQAQGTFVPLQFDERFFALRSNLQGSVSSPVEIADDLTA
                     LRLGINQRWQTKRGLPGQQRIIDWIVLDVQAVLFPDSARDNFGAAVGLVDYDFQWHVG
                     DRLTLLSDGFYDGYDQGLRQATVGALISRPEYGNVYLGFRSTEGPISSNIATGSLSYR
                     MSEKWIATAGAMWDFGSTGNIGQNFALTRIGESFLIRLGFNFDASRNNIGATFGIEPR
                     FLPNARLGRVGGVQIPPAGALGLE"
BASE COUNT         1271 a         2693 c         2927 g         1395 t
ORIGIN      
        1 gagcgaaaag aaatcgttgg cgttatgcgc gcatatccaa tctcaggcgg acggtcgaat
       61 gtcgccggcg cgcataatgc gcataatcgc ggatgcgcaa tgcgcgtcgc aaaccactct
      121 tgctgaacga cttgcggcga aaatcgcgcc gccgagaaaa catcgcggta tgcgcgcatc
      181 aagccgaagc cgccagccgc gggcggatgg gcgcggggga ggtcgtcgtg ggctgggaag
      241 cgacctggaa gattcgcacc ggcagcctgg cgtcttccgg caaggtgcgt ccgcagcggg
      301 ggcactgggc gggttttccg gggttggaga cgaccctgcg gtgagcatcg caagaccagc
      361 agtaataaac gtttacgtcg agcatggcag ccggcttccc gcggcgagca cgtggatagg
      421 agacaagtat cggcccacac ccttcgcagc ttgatcggcc gatcgttacg acccgaaaaa
      481 tcggcccgcg caagcagtag cttgccagca ggcgtttgcc gcctgcggag gggctgacta
      541 gtctgagcgt aaggcgggct gttctgttac caggcgaaaa aatgcaactg gccatactgg
      601 cggcggtgct ggcggcactc gcggcagccg agtccggcgg cggtccggtg gagggcctcg
      661 gctggcgctt gctggcgatt gcggccgtca ccctcgtcgc gccgctggcg gctttcctcg
      721 gcgggcagcg gctggcattg gacgtcgcag cgttggatcg cagcgagcgc tcgatcgcgc
      781 gattgcagtc gatcgtcgtc ggcctgtgga tgtcggccgt gctgatcgtg ctgttcatcg
      841 cgcagtggcc gcggatcgtg cgcggcaact ggcagctggc cggctggcca ctgcttgatg
      901 agctggcgat tctgacgcca gtgatcgcgc cgctgctgct cgtgtgggcc ggcctttatc
      961 ggctcgaccg cgcggcgcag gtagccgcgt tccgcgctcg cgagctggag ccgccgccgg
     1021 cgcagctcgg ccgctacctg tggctgcacg tgcggcatca cttggggctg gttttgctgc
     1081 cggcgctggc ggtcgtcggc gcattcgagc tgctgggcat gttcaatctg accagggcgc
     1141 ggccggacgc agcctggtgg ttcgcgctgc cgctcttggc gaccatgctg ctgctcatgc
     1201 cgctggcggt gcggcgcgtg tggcggacga ctccgctgcc ggtcggcccg ctgcgcgacg
     1261 agctggaggc tatctgccgc cgcgagcgct gcctggtgcg ggagatcctg gtgtggcata
     1321 ccgacggtta catggccaac gcagcggtcg tcggtctgtc gcggtggctg cggtacgtgc
     1381 tgctgaccga cgcgctcctc gcgcggctga aaactgccga gatcgtcgcc gtcctgcgcc
     1441 acgagctggc gcacctgcag cgctggcact tgccgctgcg gctagcgctc ttggcattgc
     1501 cgctggcctg gtggctggcg atcaatcaag tctggccgca cctcgatgcg gcgctgcact
     1561 atgcgatggt cgcggccggc tggcccgcgc cgctagtgac cgccgtcatg ctgcccgtct
     1621 gcatgctggc gtatgccgtg atcgtcgtgg gctggtactc gcggcttttg gagcacgaag
     1681 ccgacctggc ggcctgcctg gccgacgatg ggcaatttga ttcgctgcgg gccgccgatt
     1741 ttggccgcgc cctggtgctg ctcatcggcc gcagccgcga gagccgcctg ggccagtggc
     1801 tgcatccgtc cttgcccgac cggctgcggt tcctccgcgg tgcgatggag agggccagcc
     1861 gctgcgcgtt tgagcggcgg ctggtgtgga tcaagctgtc gatcgccgcc ggttatctgg
     1921 cggcgctcgc cgtcgcgctg ctgggctgat ttcaggaatg agccagaact tgcaggatgc
     1981 cgcggcagaa gtcgggcagg tcgtcgggct tgcggctgct gacaaagtgg cggtcgatga
     2041 cgacggaggc gtcttcccag agtgcgccgg cgttgatcag gtcgtccttg attcccggcg
     2101 agccggtgac gcggacgccg cgatagacgc ccgcggaaat cggaatccag ccgccgtggc
     2161 agatggcggc gacgagcttg ccgcccgcgg cgaaatctcg gacgagctgc aggacatcgt
     2221 tttcgcggcg gagtttgtcg ggcatgaaac cgccaggcac gacgacgccg tcaaagtcct
     2281 tggcccgctg ctcggcgatc gccgcgtcgc tgacgcaagg atagccgttt tttccggcgt
     2341 acttgtgccg cgagcgctcg ccggcgacga cgacgtctgc cccggcctcg atcaaccgga
     2401 gcttggggta ccacagctcc aggtcctcgt agatgtcacc cacgaacacg agaatgcggc
     2461 ggccggcaag cgggcgatcg gcgggcatgg gaggtttcct tcgctacggg gagttggtgt
     2521 aattcgttgt agttggttgc ccactgtgga aacaagccgc cgccaagcat gtcctctgaa
     2581 tcatcgcccg cgtccgaatc gcccgctggt cccaagtggc agcccctcac cgcgcggcag
     2641 cggcgggtgt ttggcgtgct ggtcgaaaag gccaagacga cgcccgacgc ctatccaatg
     2701 acgatcaacg gactgatcgc cggctgcaac cagaagagca accgcgagcc gcagatgaac
     2761 ctgtcggcgg aggacgtcga gcagacgctg gacgagctgc ggggcctcgg cgcggtgacc
     2821 gaagtgcagg ggagcggccg cgtggccaag taccggcatc acgcctacga gtggctgggc
     2881 gtggaaaaga cggaactggc cgtgatgacc gagctgctgc tgcgcggcga gcagacgctg
     2941 ggcgagctgc gcgggcgggc cgcgcggatg gaaccaattg ccgacctcgc gacactcaag
     3001 ccggtcgtcg attcgctcgt gaagcggggc ctgatggtcg agctgaccgc tcccggccgc
     3061 gggcaagtcg tcagccacca cctctacaag gagcgcgagc tcgcggagat caaaacgcag
     3121 ttcgccaact acgtcccgcc cgcgggctcg tcggacgatc cgccggcgat ccgcgagcgg
     3181 ccagcgcccg ccgcggcacc cgccgtcccg acggccgatc agctggccga gttgacggtc
     3241 gaggtcgccg aattgcgggc cgaggtgctg cggctgcggg accaggtgca aggtctcgag
     3301 tcgaagctgc aggccgtgat gacgtgagcc gccctctcgc gagctgtcca tgccggaact
     3361 gatcgtcgag cacgtgagca agtcgtttcc gacgcgaggc gagccgctgg tcgtgctccg
     3421 cgatgcctcg ctcacacttg cggcgggaca gaacgcggcg atcctcggac ccagcggctc
     3481 gggcaagagc acgctgctgt tcatcatcgg cacgctcgat tcgcccagca ccggcagcgt
     3541 acgactaaat ggcaccgacc cgttctcgat gcgcgaggcc gagctggccg acttccgcaa
     3601 ctgcaacatc ggcttcgtgt ttcaggatca ttacctgctg ccgcagctca ccgtgctgga
     3661 gaacgtgctc gtgccggccc tggccgcagg aacgccgccg ccggcgaccg tggagcgggc
     3721 ccgcgacctg ctggcgcggg tcggtctggc cgagcggctg gagcaccgcc cggcggagct
     3781 gtccggcggc gaacggcagc gggtcggcgt cgcgcgggcc ctgctcctca agccggcgct
     3841 gctgctggcc gacgagccta cgggcaacct cgaccgcaag agcgccgctg cgatcggcga
     3901 gctgctcgcg gaattgccgc ggcaggaaca ggccatgctg ctggtcgtca cgcacagcct
     3961 ggagctggcc caacggttcg agcggcggct ggaactggac gacggtcggc tgcgagatgc
     4021 ctgatatgac tttgctccgc gtcatcacgg gcagcctgcg ctatcactgg cgaatccacg
     4081 tcgccgtggc gctcggcgtt ttggccgcga cggcggtgct gaccggcgcg ctggccgtcg
     4141 gcgattcggt gcgctatagc ctgcggcggc tggcgctcga ccggctcggc cggatcgatg
     4201 aagttctggt ggtcgatcgc ttcctgcggc aggagctggc cggcgaactg gcttcgcagc
     4261 cgaaattcaa ggagcacttt gcctcggcga cgccggcgat cctgtttccc agcgcgaccg
     4321 ccgaatccgc gggcgaaaag cggcggctgg cggcgaacgt gcaagtcctc ggtgtcgagc
     4381 cggcgttctg ggacttgagc gtgggccact cgcggccgaa gcagtcgccc gcggacggcg
     4441 aggtgattct caacgcacca ttggcgagcg aattgaatgt gaaggtcggc gattcgcttg
     4501 tgttgcggtt cggcaaggcc gaccaggtgc cggccgacag cccgctcggc cgccgcagcg
     4561 gacaagttgc ctcgctgccg gagctgacag tcagcgagat catcccggcg gaaggcctgg
     4621 gccggttcag cctgcagccg agccaactcg cgccgcggag tgcatttgtc tcgctcgccg
     4681 ccatgcaggg tgcactcgac gtagcgggaa aagccaatac gatcttcgtc ggtggattga
     4741 atgccgatgt tcccgcgcca cctgcggcca gcggcgagct ggcgcagctg ctcacgccgc
     4801 ggctgaccga cgatggcctg gccctcaagc acgtgaagat gacgttcggc gaaggccagg
     4861 cggcccggac catctacgag tactacagcc tgacgagcga ccggatgctg ctcgacaccg
     4921 ccacggagag ggcggttcaa cgagccttcg cggcggagcg tccgcagccg ctcttgacgt
     4981 acctggcgaa cagcattcaa aagctcacgg atggcgcggc ggacgacaag gcgcgcattc
     5041 cctactcgac gattaccgcg atcgatcccg cgccgggcgg gccgcttgta gacgccgatg
     5101 gaaaacccct gccgccgctg gcagacgacg agatcgtgct gaccagctgg gcggcggagg
     5161 accagcaggc caaggtcggc gacaagattc gcgtgaccta tttcgacccg gagacggtcc
     5221 acggggcgga gcgcgaaagc tcggccgagt ttcgcgtggc ggcgatcgtg ccgctgacgg
     5281 aacccgccag cccgttcacg cgccgcacgc ccgccgtcta cgagcagccg ccgacgccgg
     5341 cgaacgatcc ggatctgacg ccggaagtga aggggatcac cgaccaggcg tcgattgcga
     5401 attgggatcc gccatttcca ttcgagcggg ggcgaattcg cccgcaggac gacgagtatt
     5461 gggagaatca ccgcacgacg cccaaggcct acgtctcgct gcggaccggc cagcggctgt
     5521 ggggaagccg cttcggcagc gtgacgagct tccgcattcc cgcaaactcg ggcgtcagcg
     5581 tggaatcgct cgagaagcaa ttcctggccg aagtcggcca aagccgcgag cggctggggc
     5641 tcgacttcaa cccgatcaag cggcagagcc tggtcgcgtc cgccgggacg acgcccttcg
     5701 acgtgctgtt cctgctcctc agcatgttca tcatcgcggc cgccctgatg ctggtctggc
     5761 tgctgttccg cctgggtgtc gagcagcggg ccagcgagat cggcctcttg ctggcgctgg
     5821 gctggtcgcg gccgcaagtc cgccggctgc tgctcctgga gggcagcgtg gtggccgcca
     5881 tcggagcggc gctgggcgtg ctgggcggca tcggctacgc ctggctgatg atcgtgggcc
     5941 tccgcacctg gtgggtcggg gcgattgcct cgccgttcct cttgctgcac gtcacgctgc
     6001 tgagcgcgac cgtcgggttc gcggcgggtt tgatcgtgtc gctcgtcacg atctggttca
     6061 gcctggggcg agtaaagaac gccgtggtga ggagcctgct ggcgggcgaa atagttccca
     6121 gcgcgaaaaa cgaatcgccg gcacttcgca ctccgcgctc cgcattccgc attcaggccg
     6181 ccgccgccgt cctcttcctc gccgccgtcg gcctggcctt ctatgccgcc acgcttggcg
     6241 gcgaggccca ggccggagca ttcatgggcg cgggcgcggc gctgcttacg gcgctgctgc
     6301 ttttcacggt ggcccagctt cgctcggccg gcggccgcag cacgctcata ggcggcgcgg
     6361 ccctggcccg gctggccttt cgcaacgcca gccgcaacgt cggccgcagt gcggcgacga
     6421 tcgccctgat ggccttcgcc gcgtttctga tcgtagcggt cagcgccttt cgcatgagcc
     6481 cgacggacac tggcgtcggc ggcttcgacc tcctggccca aagctcgcag ccgatctatg
     6541 aggacctcaa ttccgacgag ggccggcggc agctgctggc cggcaagacg gccaacgtcg
     6601 ccggctgcac gatcctctcg ctgcggctga aggccggcga cgacgccagc tgccgcaact
     6661 tgtatcagcc gtcgcagccg cagattctgg gcgtcacgcc gcaggtgatc gagtatttca
     6721 acgattcgaa actgccggcg cgctttgcct ggagcgccag cgcggcgaag tccgaggccg
     6781 aaaaggccaa tccctggcgg ctgctggcga cgccgacacc gcccaaagag ccggtgccgg
     6841 tcgtgctgga ccagaacacg gcgatgtaca gcctgcggct ctatcgcggc atcggcgaga
     6901 cgttcgacgt gacctatgac gacggcacga aggtgacctt ccgagtggcc ggtctgctgg
     6961 ccaacagtgt gctgcaaggg agcctgctga tcggcgaggc cgacttcgag cggctctttc
     7021 cgcagattag cggctatcgc tatttcctca tccgtacgcc ggcaggcaag gcagccgatg
     7081 ttgccggggt cctggaggac cagctcagcg accagggatt cgatgccacc agcgcccgcg
     7141 agcggctgac ggacctcctc gctgtgcaga acacctacat cagcacgttt cagagcttgg
     7201 gagcgctggg cctggtgctg gggacgtttg gcctggctgc ggtgcagctc cgcagcgtct
     7261 tcgagcgccg caaggagctg gcgctcttgc gggccgccgg ctttcgccgc cgccgcctgg
     7321 gccagatggt cttacttgag aatctgctgc tgcttcttgg cggcctgttg ctgggcacga
     7381 tcgccgcgct ggtcgccgtc ctgccgcaaa tgctcttcgg cggcgcgagg gtgccgctga
     7441 tcgacttggc gctcatgctc ggcgtcgtgc tcgtcgtcgg catctccacc ggcttgatcg
     7501 ccgtgcgggc gacgttgcat gcgccgctcg tcggagcact gcgcggggag tgaaggctac
     7561 tccagcccca gcgctccggc cggcgggatc tgcacgccgc cgacgcggcc gaggcgggcg
     7621 ttgggcagga agcggggctc gatgccgaag gtggccccga tgttgttgcg gctggcgtcg
     7681 aagttgaatc ccaggcgaat caggaacgat tcgccgatcc gcgtgagggc gaagttctgg
     7741 ccgatgttgc ccgtcgagcc gaaatcccac atcgcgccgg ccgtggcgat ccatttctcg
     7801 ctcatgcgat agctcagcga gccggtggcg atgttcgagc tgatcgggcc ttcggtcgag
     7861 cggaagccga gatagacgtt gccgtattcc ggccggctga tcagcgcccc gacggtcgcc
     7921 tggcggaggc cctggtcgta gccgtcgtag aagccgtcgg agagcagcgt cagccggtcg
     7981 ccgacgtgcc attggaagtc gtaatcgacc aggcccaccg cggccccgaa gttgtcgcgg
     8041 gccgagtcgg gaaagagcac cgcctgcacg tcgagcacga tccagtcgat gatccgctgc
     8101 tggccgggca ggccgcgttt ggtctgccat cgctggttga tgcccagccg cagggcggtg
     8161 aggtcgtcgg cgatctccac cggcgagctg accgagccct ggaggttgct ccgcagggcg
     8221 aagaaccgct cgtcgaactg gagcggaacg aaggtgccct gggcctggcc gaacgtgcgg
     8281 acggcc
//
LOCUS       DASRJB010000164         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3429774, whole genome shotgun sequence.
ACCESSION   DASRJB010000164 DASRJB010000000
VERSION     DASRJB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3429774"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2307)
                     /locus_tag="VFB80_04695"
     CDS             complement(<1..2307)
                     /locus_tag="VFB80_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN33090.1"
                     /translation="MKTRAIFLALLLLANLAVADDLVLNKGDHICLVGNTLAERMQYF
                     GGLESRLISRFADHDLVFRNLGYSADELTVRLRSRDFGTPDQWLAGEAPIPQPEKLDP
                     NALARPNRFELTNTKADVIFAFFGYNESFAGADGLPKFKQDLDAWIKHTLGQKYNGRS
                     APRLVLFSPIAHQNLKTPNLPTGEENNARLKLYTAAMAEIAKANGVLFVDLFAPTLEL
                     FRTSPAPLTINGIHLNEAGDALVAAIIEDALFKSGPKLKRDAAALERLRLAINDKNFY
                     WFHRYRTTDGYSTYGDRAFLKFTAGQSNYEVVQRELEVLDIETANRDRGVWSVARGGE
                     YKPADDNLPPFIPVVTNKPGPLPGGKHLFLEGEAAIDKMTVHPGMKVNLFASEEQFPE
                     LASPVQMAFDTKGRLWVAAWPTYPHWKPTTPMNDKLLIFEDTNRDGRADKVKVFAGDL
                     HNPTGFEFWNGGVIVAMAPNLLFLKDTDGDDQADQRTMLLHGLDTADTHHTSNSFTLD
                     PGGALYFQEGTFHHTQVETAWGPPRRVANGAVFRYEPRAQKFDVYVSHGFANPHGHVF
                     DRWGQDIVVDGTGAVPYHGALFSGHVDFPNKHPKPPQVYQQRMRPCSGLEILSSRHFP
                     EEMQGDLLVPNVIGDQGILRYKVLDKDASFTAVEQQPIVLSSDPNFRPADVELGPDGA
                     IYFTDWHNPIIGHMQHNLRDPNRNKTYGRVYRVTYEGRPLLTPAKIAGEPVAKLLDLL
                     KEPEDRTRYRARIELSGRPTGEVLAATKS"
     gene            2483..>2892
                     /locus_tag="VFB80_04700"
     CDS             2483..>2892
                     /locus_tag="VFB80_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZN33091.1"
                     /translation="MPTLAEMRQHLYSAVVCDALDAIGLTHQSPRVPLRPLTASGVLV
                     GRCKTTLWADMYHADPRPYELELVAVDSCRPDDVLIAAAGGTLRSGLWGELLSTAAQR
                     SGCVGVIVDGAVRDVAKMTAMGFPVFARATCLYD"
BASE COUNT          465 a          896 c          988 g          543 t
ORIGIN      
        1 gctcttcgtt gccgcgagca cttcgcccgt gggccggccg ctcagctcga tgcgggcgcg
       61 gtaccgcgtg cggtcctctg gctctttgag caggtcgagc agcttcgcaa ccggctcgcc
      121 cgcgatcttg gcgggcgtaa gcagcggccg gccttcgtag gtcacgcgat agacgcggcc
      181 ataggttttg ttacggttgg gatcccgcaa gttgtgctgc atgtggccga tgatcgggtt
      241 gtgccagtcg gtgaagtaga tcgccccatc cggccccagc tcgacgtccg ccgggcggaa
      301 gttcggatcg ctggagagaa cgatcggctg ctgctcgacc gccgtgaaac tggcgtcctt
      361 gtcgagcacc ttgtagcgca ggatgccctg atcgccgatc acgttgggca ccagcaagtc
      421 gccctgcatc tcctcgggaa agtgccggct gctgaggatt tccaggccgg aacagggtcg
      481 catccgctgc tgatagacct gcggcggctt ggggtgcttg ttcggaaaat cgacatgccc
      541 tgagaaaagc gcgccgtgat aggggaccgc tcccgtgccg tccacgacga tgtcctgccc
      601 ccagcggtcg aagacgtggc cgtgcgggtt ggcgaagccg tgcgagacgt agacgtcgaa
      661 cttctgggcc cgtggctcgt agcggaagac ggccccgttg gccacgcgcc gcggcggtcc
      721 ccacgccgtt tcgacctgcg tgtggtgaaa cgtgccttcc tggaaataga gcgccccgcc
      781 gggatcgagc gtgaagctgt tggaggtgtg atgcgtgtcg gccgtgtcca ggccgtgcag
      841 cagcatcgtc cgctgatcgg cctggtcgtc gccgtcggtg tctttcagga agagcaggtt
      901 gggggccatc gccacgatca cgccgccgtt ccagaattcg aagcccgtgg gattgtggag
      961 atcgccggca aagaccttga ccttgtcggc ccggccgtcg cggttcgtgt cctcgaagat
     1021 cagcagcttg tcgttcatcg gcgtcgtcgg cttccagtgc ggataggtcg gccaggcagc
     1081 gacccacaag cggcccttgg tgtcgaaggc catctgcacg gggctggcca attcagggaa
     1141 ctgctcttcc gaggcgaaca ggttgacctt catgcccgga tgcaccgtca tcttgtcgat
     1201 cgcggcttcg ccctccagga agaggtgctt gccgccgggg agcgggccgg gcttgttggt
     1261 cacgacgggg atgaacggcg gcaggttgtc atcggccggc ttgtattcgc cgccgcgggc
     1321 gacggaccac acgccgcgat cgcggttggc cgtttcgatg tccagcactt ccagctcgcg
     1381 ctgcacgacc tcatagttcg actgcccggc ggtaaacttc aagaacgccc gatcgccgta
     1441 ggtcgaatag ccgtccgtcg tgcggtagcg gtggaaccag tagaaattct tgtcgttgat
     1501 ggccagccgc agacgctcca gcgccgcggc gtcgcgcttc agcttgggtc cgctcttaaa
     1561 cagggcgtcc tcgatgatcg cggccaccag tgcgtcgccc gcttcgttca ggtgaatgcc
     1621 attgatcgtc agcggcgctg gcgacgtgcg gaacagctcc agcgtcggcg cgaagagatc
     1681 gacaaacagc acgccgttgg ccttggcgat ctcggccatc gccgcggtgt atagcttcag
     1741 gcgggcattg ttctcttcgc ccgtgggcag gttgggcgtc ttcaggttct ggtgggcgat
     1801 gggcgagaac aggaccagcc ggggggccga gcggccgttg tatttctggc cgagcgtgtg
     1861 cttgatccag gcgtccaggt cctgcttgaa tttcggcagg ccatccgcgc cggcgaacga
     1921 ctcgttgtat ccgaagaagg caaagatcac gtcggccttc gtgttcgtca gctcgaagcg
     1981 gttcggccgg gccagggcat tggggtcgag cttctccggc tgcgggatcg gcgcttcacc
     2041 ggccagccac tggtccggcg taccgaagtc gcgggaccgc aggcggaccg tcagctcgtc
     2101 ggccgagtag ccgaggttgc gaaacacgag gtcgtgatcg gcaaagcggc tgatcagccg
     2161 cgactccagg ccgccgaaat actgcatccg ctcggccagg gtgttaccga cgaggcagat
     2221 gtgatcgccc ttgttcagca ccaaatcgtc ggccacggcc aggttggcca gcagcaacag
     2281 cgccaggaaa atcgcccgcg tcttcatgaa catcactccg cagagagtcg cccgtcaggt
     2341 cgaaccaagt gtcgtttgtc agaagacaaa aaggaagggg ccggggaagt ggctagtgct
     2401 gggaagtggc tagtgctagt ggctagtgac taggctagtg ggtccccgtc actagccacc
     2461 agcactagcc accagcactt ccatgcccac gctcgccgaa atgcgccagc acttgtacag
     2521 cgccgtcgtt tgcgatgcgc tcgatgcgat tggcctgacg caccagtcgc cgcgcgtgcc
     2581 gctgcgcccg ctcacggcca gcggcgtgct cgtcggccgc tgcaagacga cgctctgggc
     2641 cgacatgtat cacgctgatc cgcggccgta cgagctggag ctggtggccg tcgattcctg
     2701 ccggccggac gacgtgctga tcgcggcggc cggcggcaca ctgcggtcgg gcctgtgggg
     2761 cgagctgctt tcaacggccg cccagcgcag cggctgcgtg ggggtgatcg tcgatggcgc
     2821 ggtccgcgac gtggcgaaga tgaccgcgat gggctttccc gtcttcgccc gggcaacgtg
     2881 cctgtacgac ag
//
LOCUS       DASRJB010000165         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_329865, whole genome shotgun sequence.
ACCESSION   DASRJB010000165 DASRJB010000000
VERSION     DASRJB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329865"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFB80_04705"
     CDS             <1..786
                     /locus_tag="VFB80_04705"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN33092.1"
                     /translation="GMVSELLKLTADERRDLAQRLVEFTAGRCPVFMGVGAESTRQAL
                     AYARDAATAGCDAVMATPPITGRVSESGLVSYFRALADGVNVPVIVQDASGYVGQSIP
                     LRVYVELLERYGPDKILFKPEASPIGPNLSALRDATGGQARIFEGSGGIFLIDSFRRG
                     IAGTMPAVEMADGIVAVWRALARGDEEFAYRAAAPIGAIVALQLQAGLDGFLAIEKHI
                     LVKRGVLPSSRRREPFGWELDAETAAEVERLLNQLLRVLDERR"
     gene            773..2200
                     /locus_tag="VFB80_04710"
     CDS             773..2200
                     /locus_tag="VFB80_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33093.1"
                     /translation="MNAVSWPQTRCRAGAAACDITPPVGIYHRMWGAAAHDRSTGVHQ
                     PLVATLLWLEPAEGDPSQALVIASLDHCLLDAADVELLRNSIAIQTGISPQQVQLAVT
                     HTHAAGLMSRSRADLPGGELIGPYLDGLAEKLGAVATQARERCQLASIVYGTGHCNLA
                     QNRDFYDEARQQFVCGFNPAGRADDTLLVAKVTAEGGQPLAVVVNYACHPTTLAWQNT
                     LISPDYVGGLRQTVEQHAHTPCLFLQGASGDLGPREGFVGDTAVAERNGRQLAWAALA
                     VLESLPPTGTRYVYTGPVVSGATIGVWRHEPLESPPTAWCVEKLEITLPYRAELPTLS
                     ATEAELAQARAAEAAANFAGDALLARDRRARCERLSRQLWKLKALPGGPAFPLRIALA
                     RLGDAMWLFVPGEHYQALQTTLRARFPKRPLVVATICNGWQPGYIPASAAYGQGIYQE
                     QIAVVAAGSAEAIVESLASRLASLV"
     gene            complement(2205..2651)
                     /locus_tag="VFB80_04715"
     CDS             complement(2205..2651)
                     /locus_tag="VFB80_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33094.1"
                     /translation="MRWLIVSAVLVAAGCGGPRLGKVAGKVTVAGRPVTTGTIMFTPD
                     SGPSAVGAIGPDGGYTLTTVKSGDGALVGKHRVTIESTTVGPGSLADPASFEEELKLA
                     SQPTKILVPGKVVRVVPDKYSQLSTTDLKADVAAGSNAIDFDLPRP"
     gene            complement(2681..3598)
                     /locus_tag="VFB80_04720"
     CDS             complement(2681..3598)
                     /locus_tag="VFB80_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33095.1"
                     /translation="MSSSLRRSRSAFTLVELLVVIAIIGVLVALLLPAVQAAREAARR
                     MRCQNNLRQTGLAQHNFHDVRLHFPEGMQVDVTRHCAADCRGNTMWLTALPYMEQGNA
                     ENLYNYNLGWPGAATTLTNMVQPIYICPSNGKYLAFPNRRDYFGIAGGRERHSHGWRG
                     DVFQDGLFTFNLKRRFADVTDGTSSTLAVGESMHPQRWGAGPGYGDPNIGGPVLWLMG
                     GACMQPNCKIADASYGRDYRNAKFPINATIPLLPDNENDTPFGSRHPGGANFLFADGH
                     VSFLPQSLNLLTFQNLATIGGGETIDASY"
     gene            complement(3689..>4882)
                     /locus_tag="VFB80_04725"
     CDS             complement(3689..>4882)
                     /locus_tag="VFB80_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33096.1"
                     /translation="AEVGDGTMTCFLDGDGVLRWRPGSAWQAAMPAGRRGLRRGAAFA
                     GEVVDQFVFERLGLNDITTFLTGWDEKLTPDQGLRILGDGGLAPFQPDGKPLKRVLLL
                     IHGTFSNSENFAGALASVPDTDGQSFLEWARDKYDVILAFNHPTLSLPPLLNALRLES
                     LLGDLPGKIDIVCHSRGGIVARWWYEVTCRRPERLGKVVFAGSPLAGTSLAAPDKVRD
                     VIEYFTNVGRVLKYGLGAGSLVFPFLSVAQGLMSLVCSATSFLASVPLADAVIGLVPG
                     FTAQSRVANNLELVELRNRARPMPQYYAITSDFRPTDPGWYFWRHFTRLNAASLAAGL
                     IFPKPHDLVVDHEAMTELGRTPQGKEVLIPAAHRLDYGRNGKIHHVNYFSDLKSVQFL
                     KKSLA"
BASE COUNT          792 a         1611 c         1664 g          815 t
ORIGIN      
        1 gggatggttt cggagctgct caagctgacg gcggacgagc ggcgcgacct ggcacagcgg
       61 ctggtggaat tcaccgcggg tcgatgccca gtgtttatgg gcgtcggggc cgaaagcacg
      121 cggcaggcgc tcgcgtacgc gcgggacgcc gctacggcgg gctgcgatgc cgtgatggcc
      181 acgccgccga tcaccggccg ggtaagcgag tcgggcctcg taagctattt ccgggcgctg
      241 gccgacggcg tgaatgtccc tgtgatcgtg caggatgcgt cgggctatgt cgggcagtca
      301 attccgctgc gggtgtacgt ggagctgctg gagcgctacg ggccggacaa gatcttgttc
      361 aagccggagg cttcgccgat tggtccgaac ctgtcggccc tgcgcgacgc gacgggcggc
      421 caggcccgca tcttcgaggg ctccggcgga atctttctga tcgacagctt ccgccgcggc
      481 atcgcgggga cgatgccggc cgtcgaaatg gccgacggca tcgtggctgt ctggcgggcg
      541 ctcgcgcgcg gcgacgagga gttcgcttat cgtgcggctg ctccgatcgg cgcgatcgtg
      601 gccttgcaac tccaggccgg cctcgacggc tttctcgcca tcgagaagca catcctcgtc
      661 aagcgcggcg ttctgcccag ctcgcgccgc cgcgagccgt ttggctggga attggacgcc
      721 gagacggccg cggaagtcga acggctgctg aaccaactgc tgcgggtgct cgatgaacgc
      781 cgttagctgg ccgcagaccc gctgccgggc gggcgcggcg gcctgcgaca tcacgccgcc
      841 ggtcggcatc tatcaccgga tgtggggcgc ggccgcgcac gaccgctcca ccggtgtgca
      901 tcagccgctg gtggcaacgt tgctttggct ggagcccgct gagggcgatc cttcgcaagc
      961 gctggtaatc gcctcgctcg accactgcct cttggacgcc gcggatgtcg aacttttgcg
     1021 aaactcgatt gcaatccaaa ccggcatttc gccgcaacaa gtgcagctcg ccgtcacgca
     1081 cacgcacgcg gcgggcctga tgtcgcgctc gcgggctgat ttgccggggg gcgaactgat
     1141 cgggccctat ctcgacggcc tggcggagaa gctaggcgct gtggcgaccc aggcccgcga
     1201 acgctgccag ctggcgagca tcgtctatgg cacgggccac tgcaatctgg cccagaatcg
     1261 agatttctac gacgaagccc gtcagcaatt cgtctgcgga ttcaacccag cgggtcgggc
     1321 cgacgacacg ctgctggtgg ccaaagtcac ggcggaaggc ggccagccgc tcgccgtggt
     1381 cgtgaactac gcctgccatc cgacgacgct cgcctggcag aacacgctga tcagcccgga
     1441 ctatgtgggc gggctgcggc agaccgtcga gcagcacgcg cacacgccgt gcctgttcct
     1501 gcagggcgca tcgggcgacc tcggtccgcg cgagggcttc gtcggcgaca cggccgtcgc
     1561 cgagcggaac ggccggcagc tcgcctgggc cgcgctggca gtgctcgaat cgctgccgcc
     1621 gacggggacg cggtatgtgt acaccgggcc ggttgtctcg ggcgcgacga tcggcgtctg
     1681 gcggcatgag ccgctcgaat cgccgccaac ggcttggtgc gttgagaaat tagaaattac
     1741 cctgccttat cgagccgaac ttccgaccct ctcggccacc gaggcggagc tggcgcaagc
     1801 gcgggccgcc gaggccgccg caaattttgc tggcgacgca ctgctggccc gagaccgccg
     1861 ggcccgttgc gagcggctct cgcggcagct ctggaagctg aaagctctgc cgggcggtcc
     1921 ggcgtttccg ctccgcatcg cgctggctcg cctcggcgac gcgatgtggc tgttcgttcc
     1981 gggcgagcat taccaggcgc tgcaaaccac gctgcgcgct cgattcccca agcggcccct
     2041 cgtcgtggcg acgatttgta acggctggca acccggctac attccagcga gcgcggccta
     2101 cggccagggc atctatcagg agcagatcgc cgtcgttgcc gccggcagcg ccgaggccat
     2161 cgtcgagtcc ctggcgagcc ggctggcgtc gctggtctga accgttacgg ccggggcaaa
     2221 tcgaagtcga tcgcgttgct gcccgccgcg acgtccgcct tcaggtcggt cgtgctcagc
     2281 tggctgtatt tgtccgggac cacgcggacc actttgccgg ggacgaggat cttggtcggc
     2341 tgactggcga gctttagttc ttcttcaaag ctggccggat ctgccagcga gccggggccg
     2401 acggtcgtgg actcgatcgt gacccgatgc ttgccgacca gcgcgccgtc gcccgacttg
     2461 accgtcgtta gcgtgtagcc gccgtcgggg ccgatcgcac cgacggcgga ggggccgctg
     2521 tccggcgtga acatgatcgt cccggtagtc accggacggc cggcaacggt cactttgccc
     2581 gcgacctttc ccagccgcgg tccgccgcag ccggctgcga cgagaacggc gctcacgatc
     2641 aaccaccgca tgctgagcat tctcgattcc gcctgttcaa tcagtagctc gcatcgatcg
     2701 tttcaccgcc gccgatcgtc gccagattct ggaaggtgag cagattcaac gactgcggca
     2761 ggaagctcac gtgcccgtcg gcgaacagga aattcgcccc gcccggatgc cggctgccaa
     2821 agggcgtgtc gttttcgttg tcgggcagga gcggaatggt agcattaatg ggaaacttgg
     2881 cgttgcggta gtcgcggccg tacgaggcgt cggcaatctt gcagttcggc tgcatgcagg
     2941 ccccgcccat cagccagagg acgggcccgc cgatgttcgg gtcgccgtag cccggccccg
     3001 cgccccaccg ctgcgggtgc atcgattcac ccaccgccag cgtgctcgag gtgccatcgg
     3061 tcacgtcggc gaaccgccgc ttgaggttga aagtgaacag cccgtcctgg aatacgtcgc
     3121 cgcgccagcc gtggctgtgc cgctcgcggc caccggcgat gccgaaatag tcgcgacggt
     3181 tgggaaacgc caggtacttg ccgttgctgg ggcagatata gatcggctgc accatgttgg
     3241 tcagggtcgt cgccgcccca ggccagccca ggttgtagtt gtagaggttc tcggcgttgc
     3301 cctgctccat gtacggcagc gcggtgagcc acatcgtgtt gccccggcag tcggcggcgc
     3361 agtgcctggt cacatcgacc tgcatccctt cgggaaaatg cagccggacg tcgtgaaaat
     3421 tgtgctgggc cagccccgtt tgccgcaggt tgttctggca gcgcatgcgg cgggccgcct
     3481 cgcgggccgc ctgtaccgcc ggcagcaaca gcgcgaccaa tacaccgata atggcgatca
     3541 cgaccagcag ctcgaccagc gtgaatgcag accgcgagcg gcgaagagaa cttgacatgg
     3601 cagctttcag gtgggaccga acctaggcgg gcaaagtgcc aaactagcgg atttcgctag
     3661 acatgtcaaa ggcgggtagc ggaacgaatc aggccagcga tttcttgaga aactgcacgc
     3721 tcttcagatc gctgaagtag ttgacgtggt ggatcttgcc gttgcggccg tagtcgagcc
     3781 ggtgggcggc cggaatcagc acttcctttc cctgtggcgt gcggcccagt tcggtcatgg
     3841 cttcgtggtc caccaccagg tcgtgcggct tgggaaagat cagcccggcg gcgagcgacg
     3901 ctgcattcag ccgcgtgaag tgccgccaga agtaccagcc cggatcggtc gggcgaaagt
     3961 cggaggtgat cgcgtagtac tgcggcatcg gccgggcccg gtttcgcagc tccaccagct
     4021 ccaggttgtt cgccacgcgc gactgggcgg tgaagcccgg caccaggccg atcacggcgt
     4081 cggccagcgg cacgctggcc aggaagctgg tagccgagca caccagcgac atcagcccct
     4141 gggcgaccga caggaacgga aagaccaggc ttcccgctcc cagcccgtac ttcagcacgc
     4201 ggccgacgtt cgtgaaatac tcgatcacgt cgcggacctt gtcgggagct gccaggctcg
     4261 ttccggctag cggcgagccg gcgaagacga ccttgcccag ccgctccggc cgccggcaag
     4321 tcacctcgta ccaccaccgc gcgacgatcc cgccgcggct gtggcagacg atgtcgatct
     4381 tgccgggcaa gtcccccagc agcgactcga gccgcagggc attcagcagc ggtggcagcg
     4441 acagcgtggg atgattgaag gccaggatca cgtcgtactt gtcgcgggcc cattcgagga
     4501 acgactggcc gtcggtgtcc ggaacgctgg ccagcgcgcc ggcgaaattc tcgctattgc
     4561 tgaaggtgcc gtgaatgagc agcagcaccc gcttcagcgg cttcccatcg ggctgaaacg
     4621 gcgccaggcc gccgtcgccc agaattcgca ggccctggtc gggcgtcagc ttctcgtccc
     4681 agccggtgag gaacgtggtg atgtcgttaa ggcccagccg ctcgaagacg aactggtcca
     4741 ccacttcgcc ggcgaaagcc gcgccgcggc gcagcccgcg ccgaccggcg ggcatcgcgg
     4801 cctgccaggc ggagcccggc cgccagcgga gcacgccgtc gccgtccaga aagcaggtca
     4861 tcgtgccgtc gccgacctcc gc
//
LOCUS       DASRJB010000166         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3996629, whole genome shotgun sequence.
ACCESSION   DASRJB010000166 DASRJB010000000
VERSION     DASRJB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996629"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2564)
                     /locus_tag="VFB80_04730"
     CDS             complement(<1..>2564)
                     /locus_tag="VFB80_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN33097.1"
                     /translation="RSCVVFCARKFFSAVCAHYPARLLAAVLLLACGTAWADDFPQPA
                     NQGAGAGERPLSAEQAAAGFRLPPGFKATVFAAEPDVQNPVAMTWDTRGRLWVAECYT
                     YATAQVRYDLGLRDRVLIFTDKDGDGRADERKVFCDNVQRLTSVEVGLGGVWLICLPR
                     LLFIPDRNGDDVPDGPPEVVLDGWSINSENYHNVASGLKWGPDGWLYGRCGASSPGLM
                     GPPGAPDSERVPIFGGMWRYHPKRKLYETLCHGTTNPWGHDWDAHGELFFTNTVTGHL
                     FHVLPGAHYTRSATLTVNPRVYEPIDSHADHFHFDTGRGLRDHERNGSLGGGHAHCGA
                     LIYQGRRWPAEYQGRLLTLNLHGRRINVDRLEREGAGFAARHEPDIAFAEDKWFRGID
                     LTAGPDESIYVIDWSDTGECHEKDGVHRLSGRIFRISYGEAKWPASVDLTRWPKAVLL
                     QDFENEWFVRAARRELANRAAADPWLDLSSITGNLQRWAEQADLVEVQRLRSLWTLHT
                     LGATNRASLIGLLDDRSEHIRVWALRLLVENLPLDAVFGSPGARPQALEPALVEKLLS
                     MARKDNAGLVQLTLCSILQQLPLEQRPALAMALAAHEEYAADHSLPKLLWYGLAPLAA
                     ENSAALIPIATEGKFPLTRNWISRALAEDPAKNAAALDKLLAEATGKSEDVRADVVRG
                     VAAGLAGRRKAEPPAAWAAFAASLDAAAPEVKDLVRGVSVVFGDGRALEEVRAIVLND
                     RLKVPERRAALETLIEAKPDDLRQICERLLRVRFLNATAVKGLALFDDPAIGQQLARS
                     YNSFHPVERPAVIEALVTRPGFAAALLDQIEASRIPATELSAAQARQIRSFGDEQL"
BASE COUNT          426 a          883 c          831 g          424 t
ORIGIN      
        1 tgagctgctc gtcgccgaag ctgcgaatct gccgagcctg cgccgcgctc aactcggtgg
       61 cgggaatccg gctggcctcg atctggtcca gaagcgccgc ggcaaagccg ggccgcgtga
      121 cgagagcctc gatcaccgcc ggccgctcga ccggatggaa cgagttgtag ctgcgggcga
      181 gctgctggcc gatcgccgga tcgtcgaaca gcgccaggcc cttcacggcc gtagcgttga
      241 ggaaccgcac ccgcagcagc cgctcgcaga tttgccgcag gtcgtcgggt ttggcttcga
      301 tgagcgtttc cagcgccgcc cgccgctcgg gcaccttcaa gcgatcgttg agcacgatcg
      361 cgcggacttc ttccagcgcc cggccgtcgc cgaagacgac gctgacgccg cgcaccaggt
      421 ctttcacctc gggcgcggcc gcatccagac tcgccgcaaa agccgcccaa gccgccggcg
      481 gctctgcctt gcgccgcccc gccagtccgg ctgccacgcc gcgcacgaca tcggcccgca
      541 cgtcttcgct cttgccggtc gcctcggcca ggagcttgtc gagcgccgcc gcgttcttcg
      601 ccgggtcctc ggccagcgct cggctaatcc agttgcgggt cagcgggaat ttgccctccg
      661 tcgcgattgg aatgagcgcc gcggaattct cggccgccag cggcgcgagt ccataccaga
      721 gcagcttcgg caagctgtgg tcggcggcgt attcctcgtg cgcggccagc gccatggcca
      781 gagccggccg ttgctcgagc ggcagctgct gcaggatcga gcaaagagtc aactgcacga
      841 ggcccgcatt gtccttcctc gccatcgaca ggagcttttc cacgagcgcg ggctccagcg
      901 cctgcggcct ggctcccgga gagccgaaga cggcgtccag cggcaaattc tcgacgagca
      961 gccgcagggc ccagacgcga atgtgttcgc tgcgatcatc gagcaacccg atcaacgacg
     1021 cacggttcgt ggcgcccagc gtatgcagcg tccacagcga ccgcagcctc tggacttcca
     1081 ccaggtcggc ctgttccgcc catcgttgca ggttccccgt gatactggac aagtccagcc
     1141 aaggatcggc ggctgcacga ttcgccagct cgcggcgagc agctcggaca aaccactcat
     1201 tctcgaagtc ctgaagcagt acagccttcg gccatctggt cagatcgacg cttgccggcc
     1261 atttggcttc gccatacgag atgcgaaaga tgcggcccga caggcggtgg acgccgtctt
     1321 tctcgtgaca ctcgccggtg tcgctccagt cgattacata gatgctttcg tcggggccgg
     1381 cggtcaggtc gattccgcgg aaccatttgt cctcggcgaa ggcgatgtcc ggctcgtgcc
     1441 gcgcggcgaa gcccgcgcct tcccgctcca ggcgatcgac gttgatccgc cggccgtgca
     1501 ggttgagcgt cagcagacgg ccctgatatt ccgccggcca acggcgtcct tgatagatca
     1561 gagccccgca gtgggcgtgg ccaccgccga ggctgccatt gcgctcgtgg tcgcgcagcc
     1621 cgcggccggt gtcgaagtgg aaatgatcgg cgtgcgagtc gatcggctca tagacccgcg
     1681 ggttgactgt aagcgtcgcg ctccgcgtgt agtgtgcccc gggcaacacg tgaaacaggt
     1741 ggccggtgac cgtgttcgtg aagaatagtt cgccgtgcgc gtcccagtcg tgtccccaag
     1801 ggtttgtcgt gccgtgacag agcgtctcat agagcttgcg ttttggatga tagcgccaca
     1861 ttccgccgaa gatcggcacg cgctcgctat caggcgcgcc aggcggaccc atcaacccgg
     1921 ggcttgacgc gccgcagcgg ccatagagcc agccgtccgg gccccacttc aggccgctgg
     1981 cgacattgtg atagttctcg ctgttgatgc tccagccgtc gagcacgacc tcgggcggac
     2041 catcgggcac gtcgtcgccg ttgcggtcgg gaatgaagag cagccgcggc aagcaaatca
     2101 gccagacgcc gcccaggccg acttcgacac tcgtcagccg ctgcacgttg tcgcagaaga
     2161 cctttcgctc gtcggcccgg ccgtcgccgt ctttgtccgt aaagatcagc acgcggtcgc
     2221 gcaggccaag atcgtaccgc acctgcgcgg tggcgtaggt gtagcactcg gcgacccaca
     2281 agcggccgcg cgtgtcccag gtcatggcca cgggattctg cacgtccggc tcggcggcaa
     2341 agaccgtggc cttgaagccc ggcggcaggc ggaaccccgc ggctgcctgc tcggccgaaa
     2401 gcggccgctc gccagccccc gctccctggt tcgccggctg cggaaaatcg tcggcccagg
     2461 cagtcccgca ggcgaggagg aggacggccg ccagcagacg ggcaggataa tgcgcgcata
     2521 cggcggaaaa aaacttccga gcgcaaaaaa cgacgcaact tctt
//
LOCUS       DASRJB010000167         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1173686, whole genome shotgun sequence.
ACCESSION   DASRJB010000167 DASRJB010000000
VERSION     DASRJB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173686"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2981)
                     /locus_tag="VFB80_04735"
     CDS             complement(<1..2981)
                     /locus_tag="VFB80_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN33098.1"
                     /translation="MPARHLSLGIAIAVLAAGGAAANAAPRLPRYAALLAGGQRLEGD
                     KLTDWHDTKAMPRLEGQPLLEPSNPLRWLRDRSQRLAEPPASYIEFHNGDRLPGTVID
                     YRTGQELPFDPQPAHFVVRVGVSFEPPENRAVSEIRVAAQWVRRIVWQHRGLAPSQPG
                     SAIYRDGRSLDFRAVRFHAGETHLLLADGDRRIPWSDLAELHLPAADPWMAHFDQLAT
                     LCPTLETRLIEIETAGGLLATASLARLAARFEGNSGDADRWVHGLQPAWSLDILWVPF
                     REIAYYRTWLPREVPLCRIAPRLVRQGGGVGGRRGVALNRSAVGTPLGSKSLDFGFGL
                     GAWGGSELTFDLPLGVRSLRLWVCLDRAAGTGGCIRPRIYANEAKGGPLWEGPLLVGS
                     EQVADTGAIGLAGPAGGQKAIVLQVDPIATGKPAGADPLDIRDYADWCDPLLELDPTV
                     VQAHLDQRLPRRFAAWRDWHVQIGGGTPAEAGLETTILRDERRPPPGEFLAAVQVKQR
                     PLVLSRELTIGPRDQWLLIAATRPLNRGQEPKLEVRIGGEAVAEFNVPERQADPNDNR
                     PLAVSLLGYADQQTRQIEVEIRQNAIADSAPVQYRALWTAEQLPTLFRVFEERAELAA
                     VDPTQPGTALVSGSDRHYGQHSIQVTPAGRFQLALPVRVRIRERPGWGEARFIRFAVK
                     KQGGGRVAVELEDAQPRDVAARYDLGPGKPSYGSAVRIWQDNLPKDWVVMTRDLFADF
                     GSLDAQALVVGAPDGEAAFIDHVYLARNQADFELIPAAPAAALVNEKARHELLQPLIA
                     RATPATVRIEFEGGRLASGVIVQEQGEILTAGHAVIGPNRDCRVTFADGTTAKARTQG
                     VARDFDLGLVRIQPDGRYPRLDVHTPADLPQNQPYLALMHGSRIGEEHAAAAELVELR
                     RVFRSTVWTDLGADDWLAGGPLLDQGGRLVAVQVSKSRFGGVLASRLNEAWQHAGRMR
                     NGEVFGVWPVGSEPLMDLAGIA"
     gene            3157..4059
                     /locus_tag="VFB80_04740"
     CDS             3157..4059
                     /locus_tag="VFB80_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN33099.1"
                     /translation="MSLLVVGSVALDSVQTPTHSRERILGGSATYFSYSASFFTPVRL
                     VGVVGEDFPDEHVELLRGRNIDTAGLHVVSGGKTFFWKGKYQPNMNDRETLEVQLNVF
                     GDFRPKLPEAFRRSDFLFLANGSPVTQMSVLEQAVGPRLVVADTMDLWIREQHADLMA
                     LLKRIDGLVMNDSEAKLITGDENLVAAGHKILKLGPRFVVIKKGEHGAMFFSAHETYV
                     LPAYPTERVIDPTGAGDSFAGGMMGHLAAQGNFEPKTLKEAMAYGILVASYTVEDFSL
                     DRLQSITRADLDQRLLKYRKMLSF"
     gene            4163..4486
                     /locus_tag="VFB80_04745"
     CDS             4163..4486
                     /locus_tag="VFB80_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZN33100.1"
                     /translation="MQYEEVSAEDERIATIVVDAAMKVHRALGPGLLESVYEVCLAHE
                     LRKRGLTVRRQDPIPINYDGVRFEEGFRFDLLVEERILCEIKSVQTLHPICTAQVLTY
                     LKFTN"
BASE COUNT          822 a         1488 c         1476 g          753 t
ORIGIN      
        1 gcgatgcccg ccaggtccat aagcggctcg ctgccgacgg gccagacgcc gaacacttcg
       61 ccgttccgca tccttccggc atgctgccag gcctcgttca gccgcgaggc cagcacaccg
      121 ccgaaacggc tcttggagac ttggacggca accaggcggc cgccctggtc gagcagcggg
      181 ccaccggcga gccagtcatc cgcccccagg tcggtccaga ccgtgctgcg gaagacgcgc
      241 cgcaattcca ccagctccgc cgccgcggcg tgttcttcgc cgatgcggct gccgtgcatc
      301 agggccaggt acggctgatt ctgtggcagg tcggccggcg tatggacgtc gagccgcgga
      361 tagcgaccgt caggttgaat gcgcacgagc cccaggtcga agtcgcgggc cacaccttgc
      421 gtgcgggcct tggccgtcgt gccgtcggcg aaagtcacgc ggcaatcgcg gttgggaccg
      481 attaccgcgt ggccggcagt caaaatttca ccctgttcct gcacgatgac gccgctcgcc
      541 agccgcccgc cttcgaattc gatccgcacg gttgccggag tagcgcgggc aatcagcggc
      601 tggagcagct cgtggcgggc cttttcgttc acgagtgccg cggccggcgc cgcggggatc
      661 aattcgaagt ccgcctggtt gcgggcgagg taaacatggt cgatgaacgc cgcctctccg
      721 tcaggcgcgc cgacgacaag cgcttgcgcg tcgaggctgc caaagtcggc aaacaggtcg
      781 cgagtcatga cgacccaatc tttgggcaga ttgtcctgcc agatgcgaac cgcgctgccg
      841 tacgacggct tccctggccc caagtcgtac cgtgcggcga catcgcgggg ctgagcgtcc
      901 tccagctcca cggccactcg cccgccgcct tgcttcttga ccgcaaagcg gatgaatctt
      961 gcctcgcccc agccaggccg ctcccgaatc cgcacccgca caggaagcgc tagctgaaag
     1021 cggccggcgg gtgtgacctg aatcgaatgc tgtccatagt ggcggtcgct gccgctcacc
     1081 agggcagtcc cgggctgggt cggatcgacg gcggcaagct ccgcccgctc ctcgaacacg
     1141 cgaaacaggg tcggcaactg ctcggccgtc cacagagccc ggtattgcac cggagcacta
     1201 tccgcgatgg cgttctggcg gatttcgacc tcgatctgtc gcgtttgctg gtcggcatag
     1261 ccgagcagcg acaccgccag cggacgattg tcgttcgggt cggcctgccg ctccggaacg
     1321 ttgaattcgg cgactgcttc gccgccgatt cgcacctcga gcttgggttc ctgcccgcgg
     1381 ttcagcggcc gcgtcgccgc gataagcagc cactgatcgc gcggcccgat cgtcaactcg
     1441 cggctgagca ccagcggccg ctgcttcacc tgcacggctg ccaggaattc gcccggcggc
     1501 ggccgccgct catcgcggag gatcgtcgtc tccagccccg cctccgccgg tgtgccgccg
     1561 ccgatttgca cgtgccagtc gcgccaagcc gcaaaacgcc gcggcaggcg ttgatcgaga
     1621 tgcgcttgca cgacggtggg atcgagctcc agcagcggat cgcaccagtc ggcatagtcg
     1681 cggatgtcga gcggatcagc gccagcaggc ttgcctgtcg cgatcggatc gacttgcagg
     1741 acgatggcct tttgtccgcc ggccgggccg gcgagaccga tcgcgccagt gtcggcaacc
     1801 tgctcgctcc cgacgagcag tgggccttcc cacagtggtc cgcccttcgc ctcattcgcg
     1861 taaatccgcg gccgaatgca gccgccggtg cccgcggcgc ggtcgagaca gacccacaac
     1921 cgcagcgagc ggacgcccag cggcaagtca aaggtcagct cgctgccgcc ccacgctccc
     1981 aggccaaagc cgaagtcaag cgacttgctg cccagcggcg taccgacggc gctgcgattg
     2041 agcgccacgc cgcgccgccc gccaacgccg ccgccctggc ggacgaggcg cggggcgatg
     2101 cggcagagcg gcacttcccg cggcagccag gtgcgatagt aggcgatctc ccggaacgga
     2161 acccacagaa tgtccaggct ccaggccggc tgaaggccgt gcacccagcg gtcggcgtcg
     2221 cccgagttgc cttcgaaacg cgccgcaagc cgggccagcg aagccgtcgc caacaggccg
     2281 cctgccgttt cgatttcgat cagccgcgtc tcaagtgtgg gacagagcgt cgctaactgg
     2341 tcaaagtgcg ccatccacgg atcggccgcg ggcaagtgca gctcggccag atcgctccac
     2401 ggaattcgcc gatcgccgtc ggccaagagc aagtgcgtct cgccggcgtg aaaccgcaca
     2461 gcgcgaaagt cgagcgagcg gccgtcgcgg taaatggccg agccaggctg agacggcgct
     2521 agcccgcggt gctgccagac gatgcgccgg acccactgcg cggcgacgcg aatctcgctc
     2581 accgcgcggt tctctggcgg ctcgaaactt acaccgacgc ggaccacgaa atgcgccggc
     2641 tgcggatcga agggcaactc ctgccccgtg cggtagtcga tgaccgttcc gggcaggcga
     2701 tcgccgttgt ggaactcgat gtagctcgcc ggcggctcgg cgagccgctg cgagcggtcg
     2761 cggagccagc ggagcggatt ggacggctcc aaaagcggct gcccttccag ccgcggcatg
     2821 gccttggtgt cgtgccagtc ggtgagcttg tccccctcca atcgctggcc gccggcgagc
     2881 agcgccgcat agcgcggcag ccgcggcgct gcatttgccg cagcgccgcc cgccgcgagc
     2941 acggcaatgg cgatccccag cgagagatgt cgagcgggca tcatgcaaca gtactgtagg
     3001 gagtcccgat tctgttacgc cgctcgcggc cgtgcaagct tgtgtgccgc agctggtgcg
     3061 aatccttgtg gaaatccggc cgccaccgat aatgcagcga accataggcc agcgaccggc
     3121 gctggcggcc cgcccttcat tgcgaattga ttcagcatgt cgctcctggt cgtcggttcc
     3181 gtcgctttgg acagcgttca aactcccacg cacagccgtg agcgaatcct cggcggctca
     3241 gcgacttact tttcgtattc ggcgagcttc tttacgccgg tgcgattggt gggcgtggtc
     3301 ggcgaggatt ttcccgacga gcacgtcgag ctgctgcggg ggcggaacat cgacacggcg
     3361 gggctccacg tcgtgtccgg cggcaagacg tttttctgga agggcaaata ccagccgaac
     3421 atgaacgacc gcgagacgct ggaggtgcag ctcaacgtgt tcggcgactt ccggcccaaa
     3481 ctgcccgagg cctttcgccg cagcgatttc ctgttcctgg ccaatggctc gccggtgacg
     3541 cagatgagcg tgcttgagca ggccgtcggt ccgcggctgg tggtggccga cacgatggac
     3601 ctctggattc gcgagcagca cgccgacctg atggccctgc ttaagcggat cgacgggctg
     3661 gtgatgaacg acagcgaggc aaaactgatt acgggcgacg agaacctcgt cgccgcgggt
     3721 cacaagatcc tgaagctcgg tccgcggttc gtggtcatca agaagggcga gcacggggcg
     3781 atgtttttct ccgcgcacga gacctatgtc ctgccggcgt atccgaccga gcgggtcatc
     3841 gacccgaccg gcgcggggga cagctttgcc ggcggcatga tgggccacct cgccgcgcag
     3901 ggaaacttcg aacccaagac gctcaaagag gccatggcct acggcatcct ggtcgccagc
     3961 tacacggtcg aggatttcag cttggaccgg ctgcagagca tcacccgcgc cgacctcgac
     4021 cagcggctct tgaaataccg gaagatgctg agcttttaag aggcgggcaa ggggcagcaa
     4081 gctaaacacg aaggacacga agatcacgaa gaagagatga aattgacaac tgcggacatc
     4141 ttgatgtgaa agggcaaggc gcatgcagta cgaagaagtt tcggccgagg atgagcggat
     4201 tgcgacgatc gttgtggatg cggcaatgaa ggtccatagg gcgctggggc ctggattgct
     4261 ggagagcgtg tacgaggtct gcctagctca tgaattgcgg aagcgaggct tgaccgtccg
     4321 acgacaagat ccaattccta tcaactatga tggcgtgcga tttgaagaag gattcagatt
     4381 cgatttgtta gtggaagaac gcatcctctg tgaaatcaag tccgtacaga cgctgcaccc
     4441 aatctgcacc gcccaagtcc tcacctatct taagttcacg aattgagatt ggcgttcctc
     4501 atcaacttca acgaactgtt actaaaagag ggactgcat
//
LOCUS       DASRJB010000168         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3149452, whole genome shotgun sequence.
ACCESSION   DASRJB010000168 DASRJB010000000
VERSION     DASRJB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149452"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFB80_04750"
     CDS             <1..1156
                     /locus_tag="VFB80_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN33101.1"
                     /translation="VRRLISEEGIWTGKPATNFITATANPGQENQPDPIRLAGRTARA
                     FLGMRIDCLQCHDDKLGNILVGSPGNIHDGTQADFHQLAAFFGATRISLVGVQEADQQ
                     YKTKYLKADAEEVVPAKVPFAREIFDGHGGRREQLARWVTHRENKPFARATVNRVWAL
                     LFGRPLVEPIDDIPLVGQYPPGMQLLADDFVAHKYDLQRLIRLIAASEAFRRDSRADF
                     EITEKHERAWAAFPLSRLRPEQVAGSLIQASSLTTLNGDAHILLQLAKFGQTREFVER
                     YGDLGEDEFVGRGSTIPQRLLLMNGQLVKERTQPNPLVSGSTRIAALGGSPQKQVEAA
                     YLTVLTRRPTSDELAHFTRRLADREGRNQPEALEDLYWALVNSTEFTWNH"
     gene            1246..>2351
                     /locus_tag="VFB80_04755"
     CDS             1246..>2351
                     /locus_tag="VFB80_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33102.1"
                     /translation="MGVSGLAWLTPVAERLARAAEEAPQGAPARSLIVLWMGGGPSQL
                     ETFDPHAGSPIAHQETKAIPTAARGIEIADGLPRVAEQMDKISLVRSLVSKEGDHERA
                     TYNVKTGFRPDPTLVHPSLGAIVCHQTRDNVEIPRHVSILPGAWPARGGYLGDQYDAF
                     KTYDPIGSIPDVTARVSPQRAARRLDDLQNVVEANFARGRIKNLNERTLQSTSIAAAR
                     RMMSSDQLKAFEISQAPQALRDEFGDTPFGRACLAAVQLIEVGVRCVEVELNGWDTHA
                     NNEAFVRSQCGILDPAYAALLKHLAERKLLDSTLVLWAGEFGRTPRINPAGGRDHWPH
                     GFTAALAGGGIRGARVIGETSPTPKLAENKWTEDV"
BASE COUNT          409 a          816 c          765 g          361 t
ORIGIN      
        1 cgttcgccgg ctgatctcgg aagaagggat ttggaccggc aagccggcga cgaacttcat
       61 cacggcgacc gccaatcccg gccaggagaa ccagcccgat ccgattcgcc tcgccggccg
      121 caccgcgcgg gcctttctgg gcatgcggat cgactgcctg cagtgccatg acgacaagct
      181 cggcaacatc ctcgtcggct cgcccggcaa cattcacgat ggcacacagg ccgacttcca
      241 tcagctcgcc gcgttcttcg gtgcgacccg gatttccctg gtcggcgtgc aagaggccga
      301 ccagcagtac aagaccaagt atctgaaggc cgacgcagag gaagtcgtgc cggcgaaggt
      361 gccgttcgcc cgcgagattt tcgacggcca cggcggccgc cgcgagcagc tcgcccgctg
      421 ggtcacgcac cgagagaaca aacccttcgc gcgggccacc gtcaatcgcg tctgggccct
      481 cctctttggc cggccgctgg tcgagccgat cgacgacatc ccgctggtcg ggcaatatcc
      541 gccgggcatg cagcttttgg ccgacgactt cgtcgcgcac aagtacgatt tgcagcggct
      601 gatccgcctg atcgccgcca gcgaggcatt ccgccgcgat agcagggcgg actttgaaat
      661 taccgagaaa cacgaacggg cctgggcggc gttcccgctg tcgcgcctcc ggccagagca
      721 ggtcgccggc tcgctgatcc aggcctcgtc gctgaccacg ctgaacggcg acgcccacat
      781 cctgctgcag ctggccaagt tcggccagac ccgcgagttc gtcgaacgct acggcgacct
      841 gggcgaggac gaattcgtgg gccggggcag cacgatcccg cagcggctgc tgctgatgaa
      901 cggccagctc gtgaaagaac gcacgcagcc caacccgctg gtctctggct ccacgcggat
      961 cgccgccctc ggcggctcgc cccagaagca ggtcgaggca gcgtacctga cggtcctcac
     1021 gcggcggccg actagcgacg agctggctca cttcacgcgc cgcctggcgg accgcgaagg
     1081 ccgcaatcag ccggaggcgc tagaggattt gtattgggcg ctggtcaaca gcaccgagtt
     1141 cacttggaac cactagccgg ctgcgccggg aattccatga ccaagaacca agcaccaaac
     1201 tgcgggtcgc ccgctcacct ttctcgccgt tcattgttgg gcgcggtggg cgtttccggt
     1261 ctggcttggc tgacgccggt ggcggagcga ttggcccgcg cagcggagga ggcaccgcag
     1321 ggcgcgccgg ccaggtcgct gatcgtgctt tggatggggg gtggaccgag ccagttggaa
     1381 acgttcgacc cgcacgctgg ctcgccaatc gcccaccagg agacgaaggc gattccgacg
     1441 gccgcccgag gcatcgagat tgccgacggc ctgccgcggg ttgccgagca gatggacaag
     1501 atttcgctcg tccgctcgct cgtcagcaag gaaggagatc acgagcgggc gacctacaac
     1561 gtgaagaccg gctttcgccc tgatccgacg ctcgtgcatc cgtcgctggg tgcgatcgtc
     1621 tgccaccaga ccagggacaa cgtcgagatt ccgcgtcacg tctcgattct gcccggcgcg
     1681 tggccggccc gcggcggcta cctgggcgat caatacgacg ccttcaagac ctacgatccg
     1741 atcggctcga ttcccgacgt cacggcccgg gtctcgccgc agcgggcggc ccgccggctg
     1801 gacgatctgc agaacgtcgt cgaggcgaac tttgcccgcg gccggatcaa gaatctgaat
     1861 gagcgcacgc tccagtccac gagcatcgcc gccgcccgcc ggatgatgtc ctccgaccag
     1921 ctcaaggcgt tcgagatcag ccaggcgccg caggctttgc gcgacgagtt cggtgacacg
     1981 ccgttcggcc gtgcctgcct ggccgccgtg cagctgatcg aggtcggcgt gcgctgcgtg
     2041 gaggtcgagc tgaacggctg ggacacgcac gccaacaacg aagccttcgt ccgcagccag
     2101 tgcggaatcc tcgacccggc ctatgccgcg ctgctcaagc atctggccga gcggaagctg
     2161 ctcgattcaa ccctcgtgct ctgggccggc gaattcggcc gcaccccgcg aataaacccg
     2221 gctggcggcc gcgaccactg gcctcacggc ttcaccgccg cgctggccgg cggcggcatc
     2281 cgcggggcac gcgtgatcgg cgaaacctcg cccacgccca agctggccga gaacaagtgg
     2341 accgaggacg t
//
LOCUS       DASRJB010000169         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2584957, whole genome shotgun sequence.
ACCESSION   DASRJB010000169 DASRJB010000000
VERSION     DASRJB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584957"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..646)
                     /locus_tag="VFB80_04760"
     CDS             complement(470..646)
                     /locus_tag="VFB80_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33103.1"
                     /translation="MNQPEPHPPPPSRAKAPPREAAPRDLYQVVIECSGEDQQRELFD
                     RLRREGLKVRLLVL"
     gene            complement(908..1489)
                     /locus_tag="VFB80_04765"
     CDS             complement(908..1489)
                     /locus_tag="VFB80_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33104.1"
                     /translation="MSHRLTRHEFQTIRDLLADGWRHFEIARELNLGLWTIARVADRR
                     RFVPDDIDDAEIPAAELCEDDAPPDYVAQNLRRCPECGAMVYLWPCIACSRGASAPAI
                     APLEEVDEEQEESDPPATGWVSRRIVRRKKLKQKLMNSRRLSRNGSPSTVRPEGPEGS
                     SQGREVGVVDGNSRDQGSEDRHTSAEVSALRAS"
     gene            1756..1989
                     /locus_tag="VFB80_04770"
     CDS             1756..1989
                     /locus_tag="VFB80_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33105.1"
                     /translation="MPRKEPYSGQRLEIVVERVREQATKLSTLSRTMEESQLQQVVID
                     GHAMLLRGLNQIDNFIDNANRAVREAKMAKEGF"
     gene            complement(2050..3222)
                     /locus_tag="VFB80_04775"
     CDS             complement(2050..3222)
                     /locus_tag="VFB80_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33106.1"
                     /translation="MNLFSKLRQALFGSQPRPTRLDSPDSHALRIDELPPFNLHVAEL
                     MRYDPQVRIGLGARNGLLMSAEVEVTGDDSRVVSWVQHQWNALWHQAAHQLLRAKLYG
                     FLPFEVVYRRASGGQFRGLVEVERLIDRHPSDSRLLTIGDQIVGFTVARDSVAGRPPR
                     SGGLQDLLAPQALVCTFDAECENPYGCALLARAYPAWFEKWMPGGAKRTLRLRMIKDA
                     YIGDIFWYPPDRRFELPDGREITYRELAQQIVESRASGGALTLPLIRDSQGNKQLDYT
                     PPQGTSGYTQIFRWKADLDLEIWKALEVPPEIIQASSSGSGYSGRWIPFAVALSAVHQ
                     ELAELIRCIDRDILRPIAQFNFGRQPQYQIHPKSLVETYSQMFGRSTPERSVVSGQ"
BASE COUNT          551 a         1068 c         1151 g          600 t
ORIGIN      
        1 tagtgaggcc cggagggcaa tgcaggcccg gagggcaatg caggcccgaa gggccgacct
       61 tcgtcagccc tgggcgctgg ggcgcagccg cgagcccagg gtggcgtcca acaattgacg
      121 ccgcagcccc aacggggcga aattgcgcgg gcaacgcgcc gcgccgggcg atttcgcccc
      181 gttggggctg cgcggaatcg tcatcccgta ccgtgggctg cgcccacggc tgacgaaggt
      241 cggcccgttg ggccgcagcg acaacggtca cggttcacga ggcccggagg gccggcacat
      301 cgcgcggatg ggtgccggtc ctccggacct ccgccgcgcg aactgcgttt caaaaccccg
      361 gcctgacggc cggggctatt tccttccggc cctccgggcc tcaccactga ccaccgaccg
      421 ctcacttctc catacccgtg caccgcgacc actggtcgcg gctttgagct cacagcacca
      481 gcaatcgcac cttcaaacct tcgcggcgga ggcggtcgaa cagctcgcgc tgttggtctt
      541 cgccgctgca ctcgatgacg acctggtaca ggtcgcgcgg cgcggcttct cgcggcggcg
      601 ctttcgcacg ggagggcggc ggcggatgtg gttcgggttg gttcatgcgg cggctcaatc
      661 cagggacgac gtctcatggc aggcccggag ggccgacctt cgttagccct gggcgctgcg
      721 gcgcggccgc gagcccaggg ttggcgtcca acaatcgacc ggagccccag cggggcgcaa
      781 taccagcgcc ggccgggcga tttcgcccct gcggggctgc gcggcatcgt catcgcatac
      841 cgtgggctgc gcccacggct gacgaaggtc ggcccgttgg gccgcaccga gggttcatcg
      901 acaaccgtca cgaggcccgg agggccgaca cctcagcgct cgtgtgccgg tcctccgaac
      961 cttggtcgcg cgagttgcca tcgacaaccc cgacctcacg gccctggcta ctcccttccg
     1021 gcccttcggg cctgacggtg ctgggactgc cgttgcggct tagccggcgg ctgttcatca
     1081 gtttctgctt cagcttcttc cgccggacga ttcgccggct gacccagccc gtggcgggcg
     1141 ggtcgctctc ctcttgctcc tcgtccacct cctccagcgg ggcgatggca ggcgcagagg
     1201 cgccgcggct gcaagcgatg caaggccaga gatagaccat cgccccgcac tcggggcagc
     1261 ggcggaggtt ctgagcgaca tagtcgggcg gcgcatcgtc ctcgcagagc tcggcggcgg
     1321 ggatctcggc gtcgtcgatg tcgtcgggca cgaaccgccg gcggtcggcg acgcgggcga
     1381 tcgtccagag cccgagattc agctcgcggg cgatttcgaa gtgccgccag ccgtccgcga
     1441 gcaagtcgcg gatcgtctgg aattcgtggc gcgtgagtcg gtgggacatg gaggtgggtc
     1501 agttgtcagt ggtcagttgt cagttggaga ggctttttgc ggttcctcca actggctact
     1561 gaccactgac cactgacaat gaagaatgac taatgacgaa tttgtgatga attctccaat
     1621 ttcccttgca ctctctattt ccgcggttat catcttttga tccgaaatga ctaacgagtt
     1681 cgaacagcag cgagcggcac cgaggttcac ccgatgggaa agaagaagtc cctcaaaaaa
     1741 aatgcgtccg gccgcgtgcc ccgcaaggaa ccctacagcg ggcagcggct ggaaatcgtc
     1801 gtggagcgcg tccgcgagca ggccacgaag ctctccacgc tctcccgcac catggaggaa
     1861 tcgcaattgc agcaggtcgt gatcgacggg catgcgatgc tcctccgcgg cctgaaccag
     1921 atcgacaact tcatcgacaa tgccaaccgc gccgtccgcg aagcgaaaat ggcgaaggaa
     1981 gggttttgat ttgtcagtgg tcagttgtca gttgtcagtg ggtgatgcgt tccggtctct
     2041 tccaactgac tactgaccac tgacaactga cctctccggc gtgctccgcc cgaacatctg
     2101 tgaatacgtc tccaccaggc tcttggggtg aatctggtac tgcggctggc ggccgaagtt
     2161 gaactgggcg atcggccgca ggatgtcgcg atcgatgcag cggatcagct cggccagctc
     2221 ctggtgcacg gccgatagtg cgacggcgaa cggaatccag cggccgctgt agccgctgcc
     2281 gctcgaactg gcctggatga tctccggcgg cacctccagg gccttccaga tttcgagatc
     2341 gaggtcggcc ttccagcgga agatctgcgt gtagccgctg gtgccttgcg gcggcgtgta
     2401 gtcgagctgc ttgttgccct gcgaatcgcg gatgagcggc agcgtgaggg ccccgccgct
     2461 ggcccgcgac tcgacgattt gctgggccaa ttcgcgatac gtgatttcgc ggccgtccgg
     2521 caattcgaag cggcggtccg gcggatacca gaagatgtcg ccgatgtacg cgtccttgat
     2581 catccgcagc cgcagcgtcc gcttcgctcc gccgggcatc catttctcga accacgcggg
     2641 atacgcccgc gcgagcaggg cacagccgta agggttctcg cactcggcgt cgaaggtgca
     2701 gacgagggcc tgcggggcga ggaggtcttg tagcccgcca ctccgtggcg ggcgacccgc
     2761 gacggagtcg cgggctaccg tgaaccccac gatctggtcg ccgatcgtca gcagccggct
     2821 gtcgctgggg tggcggtcga tcaggcgttc gacttcaacg aggccgcgga attggccgcc
     2881 ggaggcccgg cgatagacga cttcgaacgg gaggaagccg tagagtttcg cccgcagcag
     2941 ttgatgggcg gcctggtgcc agagggcgtt ccactggtgc tggacccagc tgacgacccg
     3001 cgagtcgtcg ccggtgactt cgacttcggc cgacatcagc aggccgttgc gagcgccgag
     3061 gccgatccgc acctgcgggt cgtagcgcat cagctcggcg acgtgcaggt tgaagggcgg
     3121 cagctcgtcg atccgcagcg cgtgcgaatc cggactgtcc aggcgcgtcg gccgcggctg
     3181 agagccgaac agcgcctggc ggagtttgga gaagaggttc atgggaatcg ggggtcgggg
     3241 gtcaggggtc gggcgttagt gatgcggtaa ttcgcggttc atgttgactg cgaagttggt
     3301 gacactcggc gtctggtcga agctttcgat ggcgccgggg actttgagat tgccattggc
     3361 gatctcgctc
//
LOCUS       DASRJB010000170         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3430959, whole genome shotgun sequence.
ACCESSION   DASRJB010000170 DASRJB010000000
VERSION     DASRJB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3430959"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..812
                     /locus_tag="VFB80_04780"
     CDS             201..812
                     /locus_tag="VFB80_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN33107.1"
                     /translation="MPVLDAQTPDGLLIAAVVAGEGEAFALLVQRYQAALLRAAVSRL
                     GRRQWAEEAVQETFLCAHKWLESYDSRFSFRTWLWTILLNQCSRLAKREQRQPAAVAE
                     AEFDVQCPAASPLEQLLARESSERLHALLSRLPEAQADALRLRFFGELTFPEIAAAMR
                     ISEPGAKHRVKTGLLKLSEWLDAPVVSSSSRAGETPAPRGVGS"
     gene            809..1492
                     /locus_tag="VFB80_04785"
     CDS             809..1492
                     /locus_tag="VFB80_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33108.1"
                     /translation="MKNLLLNCDQVFDVLTRGPFPSGDASDEGVEHHLRACHECRRLA
                     EALRPAVELLHEAVDRDEAVELPEYHGTLPLQRPLAPGVKAVAPVPRSARRFDQAVSA
                     IRLIAASLLMAALGVLAYGYAVSPPANQRDSRFGGLLGNRVPPAHLPDGPALISLASL
                     KLPARCLPAKYEALTADQAQVLAAAMASGTEEALRCCTECHAAAKPLAASTNLVAIVA
                     QNCVLCHRG"
     gene            2115..2187
                     /locus_tag="VFB80_04790"
     tRNA            2115..2187
                     /locus_tag="VFB80_04790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2148..2150,aa:Gly,seq:gcc)
BASE COUNT          392 a          771 c          797 g          407 t
ORIGIN      
        1 catcggcaca ccccgcctcg aattcgccta tcagccgcaa gactttcgcc ccttccgcga
       61 aaccggcgaa agctggcgcg tcctcacgcc cgacatcccg cagccgacaa cgtttgaacc
      121 gggcaagggt tgaaacgacc agaagtttcc cttgagcggc ccccgcggct aggataaaca
      181 gaagccaccc gaggatcgcc gtgccggtgc ttgacgctca gactcctgac ggactcttga
      241 tcgccgccgt ggtggcaggc gagggggagg cgtttgcgct gctcgtgcag cggtatcagg
      301 cggcgctgct cagggcggcg gtcagccggc tggggcgacg gcagtgggcg gaggaggcgg
      361 tgcaggagac gttcctctgc gcccacaagt ggctggagtc gtacgattcg cggttcagct
      421 tccgcacctg gctctggacg atcctgctga atcagtgctc gcggctggcc aagcgcgagc
      481 agcggcagcc ggcggccgtg gcggaggctg agtttgacgt ccaatgcccg gcggcctccc
      541 cgctggagca attgctggcc cgcgagagca gcgagcggct gcacgcactc ctgtcgcggc
      601 tgccggaggc ccaggccgac gccctacggc tgcgattctt cggcgaattg acgttcccgg
      661 aaatcgccgc ggcgatgcgg atcagcgagc cgggagccaa gcaccgggtg aagacgggac
      721 tgctcaaatt gtcggaatgg ttggacgcgc ctgtggtgag ttcgagttcg agggccggcg
      781 agacgcccgc cccacgtgga gttgggtcat gaagaatctg ctgctgaact gcgatcaggt
      841 gttcgacgtg ctgacgcggg ggccgtttcc ctcgggcgac gccagcgacg agggcgtgga
      901 gcaccacttg cgagcctgcc acgagtgccg ccggctggcc gaggccctgc ggccggcggt
      961 cgagctgctg cacgaagcgg tcgatcgcga cgaggcggtg gagctgcccg aatatcacgg
     1021 cacgctgccc ctccagcggc cgctggcccc gggcgtcaag gcggtggctc cggtcccgcg
     1081 ctcggcccgc cgctttgacc aggccgtgag cgccatccgc ctgatcgcgg cttcgctgct
     1141 gatggctgcg ctgggcgtgc tggcgtacgg ctatgcggtc tcgccgccgg caaaccagcg
     1201 cgacagccgt tttggtggcc tgctgggcaa ccgcgtgccg cccgcgcacc tgcccgatgg
     1261 gccggctctg atttcgctgg cgtcgctcaa gctgccggcc cgctgcctgc ctgccaaata
     1321 cgaggcgctc accgccgacc aggcccaggt cttggccgcc gcgatggcca gcggcacaga
     1381 ggaggccctc cgctgctgca ccgaatgcca cgcggcagcc aagccgctcg cggccagcac
     1441 gaacctcgtc gccatcgtcg cccagaactg cgtcctgtgc caccggggct gattcggcgc
     1501 ttcttgacct tacgtgtaca ggttgataca attcgcgttc ggctggccct cgccgcgttc
     1561 cggcgagtga cgttctttga caagtgatgg tgaccctctc gctctggtgc tgcgcgcata
     1621 atgcgcataa tcgctggcgg ggttttccag gtgcaaccgc tgacgccaca acgacttgcg
     1681 gcggaaaacg ggtgccggga aaatctccgc gagatgcgcg catagttgtc ctgggacgat
     1741 gcaacgtgcg acgacgcgct ggtgctggcg cgcataaagc gcataatcgc cggccccgga
     1801 tttcagccgc aactaatgac ggggaaagca cttgcggcga aaacttggcc ccagagagat
     1861 cgttcgagta cgcgcgcatg agctggcagc ggcaccggcc aatcggcggc gagctagctg
     1921 gtccggtcga accaaccgcc gcggttggcg gtctcttcgc aagagtcgca ctgcccagcg
     1981 tgccgatgga aattcgaggt cgaaaaatcg tcacgagacg ttcgccgctc ggcaaagtcg
     2041 cggcgtgagc ggcccacttt aagcccggct ttacaaccgg gtcgaatccg taacaattac
     2101 gagtcttgcg agccgcgggt gtagctcagt tggtagagcg ccaccttgcc aaggtggctg
     2161 tcgtgggttc gagtcccatc acccgctctg aacggtgggg ttgccggttg gcggccccta
     2221 aactgcccgc aatttgcccg aagcgccggc gaggcatgcc ccttgctgtc tcttcgggtt
     2281 tgttgttaat ggcacgattt ctcgcccgcc aaaaaaggcc ctcccatgag caccaccgaa
     2341 gtcgatgaag tcgccggtca gtccgag
//
LOCUS       DASRJB010000171         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3714521, whole genome shotgun sequence.
ACCESSION   DASRJB010000171 DASRJB010000000
VERSION     DASRJB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3714521"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFB80_04795"
     CDS             <1..468
                     /locus_tag="VFB80_04795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN33109.1"
                     /translation="QAALKEHLRLVELPSHQALPDLEREGIRAGLVFIDGAHLTDYVF
                     ADALGADRLLTAPGLLAFDDSDWPAVQTVIRYFLANRHYQPAHPEVVIEPPPGRPSLA
                     GRLLRRLPLIHDRLRSEFARPAAELGILGRCVVLRKLKDDDRNSQDPAAHRAY"
     gene            481..1800
                     /locus_tag="VFB80_04800"
     CDS             481..1800
                     /locus_tag="VFB80_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71/99-like protein"
                     /protein_id="HZN33110.1"
                     /translation="MHRLKCLALLLVAAHCAAADPPTREAVIAEMKPYAGTSAAGVDR
                     STLTGKVMAGYQGWFTAEGDGAELGWRHYSNRGQFRPGRCNIDLWPDVSELGADEKIA
                     TQFRHADGQPAFVFSSHNRKTVLRHFQWMKDYGLDGVFVQRFVVETQAALSLRHCNTA
                     LAHCREGANLHGRCYAAMYDLSGLREGGIDRAIDDWKLLVDRMQLGRDENDKAYLRHN
                     GQPVVAVWGIGFNDGRAYTLAECDRLVEFLKNDPTYGGNTVMVGIPTNWRTLDRDTIA
                     DPALHATLLKADILSPWTVGRFATPQAAARHARERMQPDIAWCREHGKEYLPVVFPGF
                     SWHNMRGRRRQAPFDQIPRLKGEFLWRQIAEAKNAGATMLYTAMFDELDEGTAIFKTS
                     QSPPTGESRFIADAELPSDHYLWLTGQGGRVLRGEIPSSEKIPLRSP"
     gene            1835..3211
                     /locus_tag="VFB80_04805"
     CDS             1835..3211
                     /locus_tag="VFB80_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33111.1"
                     /translation="MRGVFSNRVPRREVLRVGGLFGMGLALPTLLAQRTGLGAGAKQG
                     STFGRAKQVIVLYLHGGHPQQETFDPKPSGPSAVRGEFGAIPTSLAGVQFSELLPQSA
                     QLAHRLAIVRSMSHGNANHVTASLPANSGHAHPPGTPETDFPPAATDFPPFGAVLDRV
                     RPAARDLPTWVRVGPLMRRNNGTVLHGQLPGFLGPSHASFDVDQQLLAKDVRIQAVER
                     AGGMTALRMTERRDLLSEFEQTRRLIDHSAQARDLNAYYQRAFALLTSDRTRQAFELA
                     AEPAQVRDRYGQTEFGQRCLLARRLAAAGVPMINVSYCHTPVGSWDTHSQNFKQMKES
                     LAPTFDAAFSALIEDLADRGMLDETLVLVNAEFGRTPVINKSAGRDHWPWVYSLVLAG
                     AGIKAGTVYGASDNSAAYPTDKPHDPRDMAATIYHLLGVDPQTTIYDQTGRPHPLVIG
                     KPIEGVLA"
BASE COUNT          607 a         1251 c         1202 g          582 t
ORIGIN      
        1 caagctgcat tgaaagagca cctgaggctc gtcgagctgc cttcccacca ggcgctgccc
       61 gatctggagc gggagggcat tcgagcgggc ctggttttca tcgacggcgc tcacttgacg
      121 gactacgtgt ttgccgacgc cctcggcgcc gaccggctgc tcaccgcgcc aggcctcttg
      181 gcgttcgacg actccgactg gccagccgtg cagaccgtca ttcgatactt tctggccaac
      241 cggcactatc agcccgccca tccggaagta gtgatcgagc cgccgcccgg ccggccttcg
      301 ctggccggcc ggctgctgcg gcgcctgccg ctgatccacg atcgcctgcg ttccgaattt
      361 gcccgcccgg cggcggagct gggaatcctg ggccgctgcg tcgtcctgcg caagctgaag
      421 gacgacgacc gcaacagcca ggaccctgcc gcccaccggg catattgaaa tgccatcctc
      481 atgcaccgcc tgaaatgcct ggccctgctg ctcgtcgcag ctcactgcgc tgctgctgac
      541 ccgccaacgc gcgaggcggt catcgcggag atgaaaccgt acgccggaac ttccgcggcc
      601 ggcgtggacc gcagcacgct caccgggaag gtgatggccg gctatcaggg ctggttcact
      661 gcggaaggcg acggcgcgga gctgggctgg cggcactatt ccaaccgcgg ccagttccgg
      721 ccggggcggt gcaacatcga tctctggccc gacgtgagcg agttgggcgc cgacgagaag
      781 atcgcgacgc aatttcggca cgccgacggc cagccggcgt ttgtcttcag ctcgcacaac
      841 aggaagactg tgctgcggca cttccagtgg atgaaggact acggcctgga cggcgttttc
      901 gtgcagcggt tcgtcgtcga gacgcaagcg gccctgtcgc tgcggcactg caacacggcg
      961 ctggcccact gccgcgaggg cgcgaacctg cacggccgct gctatgccgc gatgtacgac
     1021 ctgtcgggcc tgcgcgaagg cggcatcgac cgggcgatcg acgactggaa gctgctggtc
     1081 gatcggatgc agctcggccg cgacgaaaac gacaaagcct atctgcggca taacggccag
     1141 ccggtcgttg cggtctgggg catcggcttc aatgacggcc gggcctacac gctggccgag
     1201 tgcgaccgcc tggtcgagtt cctgaagaac gacccgacct acggcggcaa cacggtgatg
     1261 gtcggcatcc cgaccaactg gcggacgctc gaccgcgaca cgattgccga tccggcgctg
     1321 cacgcgacgc tgctcaaggc cgacatcctc agcccctgga cggtcggccg cttcgccacg
     1381 ccccaggccg ccgcccgcca cgcccgcgag cggatgcagc ccgacatcgc gtggtgccgc
     1441 gagcacggca aggagtattt gcccgtcgtc tttcccggct tcagctggca caacatgcgg
     1501 ggcaggcggc ggcaagcgcc cttcgaccag attccgcggc tgaaagggga attcctctgg
     1561 cggcagatcg ccgaggcgaa aaacgccggc gcgacgatgc tctacactgc catgttcgac
     1621 gagctggacg agggcacggc gatcttcaag accagccagt cgccgcccac cggcgaaagc
     1681 cgcttcatcg ccgacgccga gctgcccagc gatcactacc tctggctgac gggccaaggc
     1741 ggccgggtgc tccgcggcga aattccttcg agtgaaaaga ttccgctgcg ttcgccctag
     1801 gcatcgggta tagtgcgctg acggggagtt tgccatgcgc ggcgttttta gcaaccgcgt
     1861 accgcgacgc gaagtgctgc gggtcggcgg tctgttcggg atgggtctgg cgctgccgac
     1921 cctcttggcg cagcggaccg gcttgggggc cggggccaag cagggatcga cgtttgggcg
     1981 agcgaaacag gtcatcgttc tctatttgca cggcggccat ccgcagcagg aaacgttcga
     2041 tcccaagccg agcggccctt cggcggtccg cggcgagttc ggagcgatcc cgaccagcct
     2101 ggccggcgtg cagttcagcg agctgctgcc gcagtcggcc cagttggccc accggctggc
     2161 aatcgtgcgg tcgatgtcgc acggcaacgc gaaccacgtg acggccagct tgccggccaa
     2221 ttcgggacat gcgcacccgc cgggcacgcc cgagaccgac tttccgccgg ccgcgaccga
     2281 tttcccgccg tttggcgcgg tcctcgatag ggtccgcccc gcggcgcgcg acctgcccac
     2341 ctgggtccgc gtcggcccgc tgatgcggcg gaacaacggc accgtgctgc acggccaact
     2401 gcccggcttc ctcggcccgt cgcacgccag cttcgacgtc gatcagcagt tgctcgccaa
     2461 ggacgtgcgg attcaagcgg tcgagcgggc cggcggcatg acggccctgc ggatgaccga
     2521 gcgccgcgac ctgctttccg agttcgagca aacgcggcgg ctgatcgacc actcggccca
     2581 ggcccgcgac ctgaacgcct actaccagcg ggccttcgcc ctgctcacgt cggaccgcac
     2641 gcggcaggcg ttcgaactgg ccgcggagcc agcgcaggtt cgcgaccgct acggccagac
     2701 cgaattcggc cagcggtgcc tcttggcccg ccggctggcg gcggcgggcg tgccgatgat
     2761 caacgtcagc tactgccaca cgccggtcgg ctcgtgggac acgcacagcc aaaacttcaa
     2821 gcagatgaag gagtcgctcg cgccgacgtt cgacgcggcg ttttcggcac tgatcgaaga
     2881 tctcgccgac cgcgggatgc tcgacgagac gctggtcctg gtgaatgctg agtttggccg
     2941 cacgccagtc atcaacaaaa gcgccggccg cgaccactgg ccctgggtct attcgctggt
     3001 gctcgccggc gcggggatca aggccggcac ggtttatggg gccagcgaca actccgccgc
     3061 ctatcccacc gacaagccgc acgacccgcg cgacatggcc gccacgatct accatctgct
     3121 gggcgtcgat ccgcagacga ccatctacga ccagaccggc cggccgcatc cgctggtcat
     3181 cggcaagccg atcgagggcg tgctggcgtg agcattggtt agcttggcgg gcgggcttgg
     3241 cggcgcgaat atcagccccc tggttgggcg agtgcgatcg cgcctgtttt ggcatcttat
     3301 tgtcgcaact cctgaagtgc cataggcttg cagcaaaggc gtccgcaggc ggttaagtac
     3361 tacttattag tgtttgagaa cccccttcgc cgggccaccc tagcggcgct cttcaccagg
     3421 aacgacgtct gattgcaggc ccggagggcc gaccttcgtt agccctgggc gctgcggcgc
     3481 agccgcgagc caaacgatcg tgcccgtggc cgctggcggg ctgccggccc gacgctcgcc
     3541 gccgggtact cagtacggca cggggcggtc gggcgaccag acatcaacta tatccacacg
     3601 tggacagaat tatcacatcg gcccgagaat tgcaggcaac tt
//
LOCUS       DASRJB010000172         8034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2304020, whole genome shotgun sequence.
ACCESSION   DASRJB010000172 DASRJB010000000
VERSION     DASRJB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304020"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFB80_04810"
     CDS             <1..636
                     /locus_tag="VFB80_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN33112.1"
                     /translation="DGRWACHRKLHVFVSEFLTPGDRYTVFDTPHGCRVGVLICYDNN
                     LVENARITALMGAEILLAPHQTGGCRSGSPCAMGPIDVELWENRRHDPDALRAEFQGD
                     KGRGWLRRWLPARAHDNGMFLVFANGVGRDDDEVRTGNAMILDCYGRTVAESQSIEDD
                     LVVGDLDAGLRERSTGVRWIKARRPELYQPLVQRSGKEMDTRSVRFEYDAE"
     gene            641..1825
                     /locus_tag="VFB80_04815"
     CDS             641..1825
                     /locus_tag="VFB80_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN33113.1"
                     /translation="MFDLAQIQAALRQFKLDGWLLYDFRGLNILARRIVDLPADQMGS
                     RRWFYFVPASGQPRKLVHRIESGALDHLPGEKVVYLKWQELEAGVQQLVGGAKRVAME
                     YSPRNANPYVSRVDAGTFELVRSFGAEIVSSGDLIQLFEATWDDEQIAMHLAAAVHTN
                     SAYAKAWRFIAEQVQARGGVRETAVQAHIMQHFGDNGLTTYHPPIVAANAHSGDPHFD
                     TSPATDALIKSGDFVLIDLWAKLERPRSVYSDLTRVGFVGRTVPEKYDRIFQIVAAAR
                     DAAIAKIKSAFAAKQPIAGWQVDNACRAVIEQAGFGANFIHRTGHSIGQEVHGNGANI
                     DGLETRDERTLLPRTCFSIEPGIYQSEFGVRSEVNVLIDPAGQVQVTGGELQKSVVPI
                     CQ"
     gene            1910..3652
                     /locus_tag="VFB80_04820"
     CDS             1910..3652
                     /locus_tag="VFB80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HZN33114.1"
                     /translation="MKYSLNLGALKSTLPPRAAGGKDVFRLAVLGDFSGRASGGQLAT
                     GDELAKRKPLAVDVDNLDELVQRMNIKLTLPIGAEGGAVEIPIASMDDFHPDQLYDNV
                     EAFSALSGLRQRLKTASMFAKAAAEVQSWSGASAAEPEPRPKARGSVVPVNGKLSDFA
                     ALIGQPSAKETETPIADLLKQIVGPHVVAAKDPKADKLVATVDQAIGQTMQSILHHPD
                     FQALESVWRGLEFLVRRLETGQSLRIVVYDISAEEFAADLSATEDLQETGLYKLLVEQ
                     PALDAQQGPITAIVTNYFLEKTPPHAELLGRMSRLCAAASAPLLAAIGTDVIKKLKPE
                     EIHPLIKESWGALKSMPEAGYLGLTVPRFMLRNPYGAKTDPIDKFDFEEFTPKEGLKG
                     MLYTSGAVLAGLLLAQTYEKAGSVKKISLGGVMSAGDMPYYYYEDADGDQVALPCTER
                     LLSVDAAAHVSAQRFMPVLAIKGRPEVRLGSFQSVTGKPLAGLWAPVTIAPTAPAAAP
                     EPAAPPPAAAAPAAAEQPAAPAAESPPAEAAASADAELDALLASLGTPAEAAPAAGGE
                     AAIDPDLAALLADL"
     gene            3665..4252
                     /locus_tag="VFB80_04825"
     CDS             3665..4252
                     /locus_tag="VFB80_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33115.1"
                     /translation="MFGRFLSILKDLVRFFSGTGSSGASPEKRPSESRLDSTAEEWRR
                     EVLSLGLPPVFADVWDDRVPSVLVAWRRPDTFLRRRADLLRYLPRLACCVPLCQEFGD
                     TLFAYDRESDEFVACHFALGEPPYLEKMGDSYQRFVAAYLVDCVYAGEERLEEIAAIL
                     NFSHLKTILTFAEQEDDDLDPDQAKQQLVASIPKN"
     gene            complement(4255..5262)
                     /gene="msrP"
                     /locus_tag="VFB80_04830"
     CDS             complement(4255..5262)
                     /gene="msrP"
                     /locus_tag="VFB80_04830"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZN33116.1"
                     /translation="MFVHQPDPLAPRGGQETPERVFLNRRSWLRLAAYGGAAIVGGAG
                     YAMWRRSANGQDDEVIAAGRWSPESEQKYASFYPARRDERFTYGRAETIAAEAARYTN
                     FYEFSRFKWCWKYVQRFRPEAWTITIGGLCRNPLTLDLDAFHKLFQSELVERQYRHRC
                     VERWAMAIPWTGIPLAALVKAADPLAQATHVRLVSFQRPSEAPHQAEGGEFPWPYTEG
                     LTLAEATNELPLLAVGVYGRPLLKQHGAPLRLVVPWKYGYKSIKSVEFIEFVGREPAT
                     FWNTMNPAAYPFESNVDPAVHRPWNQAYETMLGTGERRPTEKFNGYGQWVAGLYQSSD
                     A"
     gene            complement(5692..7251)
                     /gene="guaA"
                     /locus_tag="VFB80_04835"
     CDS             complement(5692..7251)
                     /gene="guaA"
                     /locus_tag="VFB80_04835"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZN33117.1"
                     /translation="MTASTAASQERVLVLDFGSQFAQLIARRVRENRVYCEIVRHDLS
                     PEQIRRHNPRGLILSGGPASVYEAGAPKCDPAIFRLGIPVLGICYGMHLACEVLGGKV
                     ESFPAREFGRARCRVTQPSDLFAGVPEQFDVWMSHGDQVMRVSDDFTPLAKTGTCPIA
                     AVKHKSLPIYCVQFHPEVTHTAIGSHLLHNFLMTVCGCTGTWKLGDFAKETIRAMRER
                     IGQHRVICGLSGGVDSAVTAALLYQAIGSQLSCILVDNGLLRKDEQAAVIAEFTRHFK
                     TDLHVVQGEPRFLADLKGITEPQEKRRRIGHAFIECFKEEAKQIQGVKFLAQGTLYPD
                     VIESGAAADGPAATIKLHHNVGGLPAELGFELVEPLRDLFKDEVRRLGLELGLPEDLV
                     WRHPFPGPGLAVRCLGEVTKQRLDVLREADAIMIEEIKAAGLYRQTSQAFAVLLPVQS
                     VGVMGDARTYENAVALRCVNTDDFMTADWSHLPYDVLARISTRIINEVKGVNRVCYDI
                     SSKPPATIEWE"
     gene            7374..7919
                     /locus_tag="VFB80_04840"
     CDS             7374..7919
                     /locus_tag="VFB80_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZN33118.1"
                     /translation="MADSKNPPRRWLWYALAGGFLIVVISCTGLIGSYRIWRTRQQAQ
                     QREAVTAVLALGGTAQQTLSSTSPISVLMEQGDAPNHITLNELALEDDDLRLFGSAPT
                     TRGLFLFKNEITDQGLVHLRDLKSLETLDLRRNTRITDAGLVHLEGLTNLKHLYLIGT
                     GVTPAGVAKLQKKLPKTKIAY"
BASE COUNT         1415 a         2630 c         2625 g         1364 t
ORIGIN      
        1 gacggccgct gggcctgcca ccgcaagctg cacgtcttcg tcagcgaatt cctcacgccc
       61 ggcgaccgct acaccgtgtt cgacacgcct cacggctgcc gcgtcggcgt gctcatctgt
      121 tacgacaaca acctcgtcga aaacgcccgc atcacggcct tgatgggggc ggaaatcctg
      181 ctcgccccgc atcagaccgg cggctgccgc tcgggcagcc cctgcgcgat ggggccgatc
      241 gacgtcgagt tgtgggagaa ccgccggcac gatcccgatg ccctgcgggc cgagtttcag
      301 ggcgacaagg gccgcggctg gttgcggcgg tggctgccgg cgcgggcgca cgacaacggt
      361 atgttcctgg tttttgccaa cggcgtcggc cgcgacgacg acgaggtccg cacgggcaac
      421 gcgatgatcc tcgactgcta cggccgcacg gtcgccgaat cccagtcgat cgaggacgac
      481 ctggtcgtag gtgatctcga cgccggcctg cgcgagcgct cgactggcgt ccgctggatc
      541 aaggcccgcc gcccggagct gtatcagccg ctggtgcagc ggagcggcaa ggagatggat
      601 acgcggagcg tgcggttcga gtacgatgcg gagtaagttc atgttcgacc tcgcccagat
      661 tcaggccgcc ctgcgacagt tcaagctcga cggctggctg ctctacgact tccgcggact
      721 caatatcctc gcgcggcgga tcgtcgatct gccggcggac cagatgggct cgcggcggtg
      781 gttctatttc gtgcccgcca gcgggcagcc gcgaaagctg gtgcaccgga tcgagagcgg
      841 ggcgctggac cacctgccgg gcgagaaggt cgtctatttg aagtggcagg agttggaagc
      901 gggcgtgcag cagcttgtcg gcggcgcgaa gcgcgtggcg atggagtatt cgccgcggaa
      961 cgccaatccg tacgtatcgc gggtcgatgc cgggacgttt gaactggtcc gcagcttcgg
     1021 cgcggagatc gtgtcgtcgg gcgatctgat ccagctcttc gaggcgacgt gggacgacga
     1081 gcagattgcg atgcacctgg cggcggcggt gcacacgaac tctgcctacg ccaaggcctg
     1141 gcggttcatc gccgagcagg tgcaagctcg cggcggcgtg cgcgagaccg cggtgcaagc
     1201 ccacatcatg cagcactttg gcgataacgg cctgacgacc tatcatccgc cgatcgtcgc
     1261 cgccaatgcc cacagcggcg atccgcactt cgacacgtcg ccggccacgg acgccctaat
     1321 caagagcggc gacttcgtgc tgatcgacct gtgggccaag ctcgagcggc cgcggagcgt
     1381 ctatagcgat ttaacccgcg tcgggtttgt cggccgcacg gttcccgaga aatacgatcg
     1441 catcttccag atcgtcgccg cggcccgcga cgccgccatc gccaagatca aatcggcctt
     1501 cgctgcgaag cagccgatcg cgggctggca ggtcgataac gcttgccgag cggtgatcga
     1561 gcaggccggc ttcggggcga atttcattca ccgcaccggc cacagcatcg gccaggaagt
     1621 gcacggcaac ggggcgaaca tcgacgggct ggagacccgc gacgagcgga cgctcttgcc
     1681 gcggacctgc ttctcaatcg agccgggcat ttatcagagc gaattcggcg tccgcagcga
     1741 ggtcaacgtc ttgatcgatc ccgccggcca ggttcaggtc accggcggcg agctgcagaa
     1801 gagcgtcgtg cccatttgtc agtagtcagt tgtcagtgga atcaattgac aacttgcaac
     1861 tgaccactga caactgacaa ttgccccaat cgaacgaggc tatttagcca tgaaatactc
     1921 cctgaacttg ggcgccctga aatcgacact gccgccgcgg gcagcggggg gcaaagacgt
     1981 ctttcgcctg gcggtgctgg gcgatttctc ggggcgggcc agcggcgggc agctggcgac
     2041 gggcgacgag ctggcgaagc ggaagccgct agcggtcgat gtcgataacc tcgacgaact
     2101 cgtgcagcga atgaacatca agctcacgct gccgatcggc gcggagggcg gggccgtcga
     2161 gatcccgatc gcctcgatgg acgattttca tccggaccag ctgtacgaca acgtggaagc
     2221 gttcagcgcg ctgtccggcc tgcggcagcg attgaaaacc gcctcgatgt tcgccaaggc
     2281 ggcggccgaa gtgcagagct ggtcgggcgc aagcgcggcg gagcccgagc cgcggcccaa
     2341 ggcccgcggc agcgtcgtac ccgtcaacgg caagctcagc gatttcgcgg ccctgatcgg
     2401 gcagccttca gcgaaagaga cggaaacgcc gatcgccgat ctgctgaagc agatcgtcgg
     2461 gccgcacgtc gtggcggcca aggacccgaa ggccgacaag ctggtggcca ccgtcgatca
     2521 ggccatcggc cagacgatgc agagcatcct ccatcatccc gatttccagg cgctggaatc
     2581 ggtctggcgc ggcctggaat tcctggtccg ccggctggag acggggcaga gcctgcggat
     2641 cgtggtgtac gacatctcgg ccgaggaatt cgccgccgat ctttccgcta cggaggactt
     2701 gcaggagacc ggcctttaca agctgctggt cgagcagccc gccctcgacg cccagcaagg
     2761 cccgatcacg gcgatcgtca ccaactattt cctggaaaag acgccgccgc atgccgagct
     2821 gctcggccgc atgtcgcggc tctgcgctgc cgcgagcgcg ccgctgctcg cagcgatcgg
     2881 cacggacgtg atcaaaaagc tcaagcccga ggaaatccat ccgctgatca aggagagctg
     2941 gggagcgttg aagtcgatgc cggaagcggg ctacctcggc ctgaccgtgc cgcgattcat
     3001 gctccgcaat ccttacggcg cgaagaccga tccgatcgac aagttcgact tcgaggagtt
     3061 cacgccgaag gaaggcctca agggcatgct ctacaccagc ggcgcggttc tcgcggggct
     3121 gctgctggcc cagacctacg agaaggccgg ctcggtgaag aagatcagcc tcggcggcgt
     3181 aatgagcgcc ggcgacatgc cgtattacta ctacgaagac gccgacggcg atcaggtcgc
     3241 cctgccctgc accgagcggc tgctctcggt cgatgccgcg gcgcacgttt ccgcccagcg
     3301 gttcatgccg gtgctggcga tcaagggccg gcccgaggtc cgcctgggca gctttcagtc
     3361 tgtcaccggc aagccgctgg ccggcctctg ggcgccggtg acgatcgcgc cgacagctcc
     3421 tgctgccgcg cccgagccag ctgctccgcc gcccgcagcc gccgcacccg ccgccgccga
     3481 gcagccggcg gcaccagcag ccgagtcgcc gccggccgaa gccgcggcat ccgccgatgc
     3541 cgagctcgac gccctgctgg ccagcctagg cactcccgcc gaagcggcac ccgccgccgg
     3601 cggcgaagcc gccatcgacc ccgacctggc ggcgctgctc gcggatttgt gattcctcga
     3661 ttcgatgttc ggacgctttt tgtccatcct gaaagatctc gtccggttct tttctggaac
     3721 tggctccagc ggtgcatcgc ccgaaaaacg gccaagcgag agccgactcg acagcacggc
     3781 ggaggagtgg cgccgagagg tcctcagtct ggggctgccg ccggtttttg cggatgtctg
     3841 ggacgaccgt gttccaagcg tcttggtcgc ctggaggcga ccggacacgt tcttgcgccg
     3901 ccgagctgac ttgctgcgct acttgccgag acttgcctgc tgcgtgccgc tttgtcagga
     3961 attcggagat accttgtttg cctacgatcg ggaatcagac gaattcgttg cttgtcattt
     4021 cgcgctgggc gaaccaccct atctcgaaaa aatgggcgac agttatcagc ggttcgtcgc
     4081 cgcgtacctc gtcgattgcg tttacgccgg cgaagaacgg ttggaagaga ttgctgcgat
     4141 tctgaacttc agccacctca agacgattct gacctttgcg gagcaagagg acgacgatct
     4201 cgatccggac caggccaaac agcaactagt cgcttcgatt ccgaagaact gacttcacgc
     4261 atcggacgat tggtacaggc ccgcgaccca ctggccgtag ccgttgaatt tctcggtggg
     4321 ccggcgctcg ccggtgccga gcatggtctc gtaggcctgg ttccaggggc gatggaccgc
     4381 tggatcgaca ttgctttcga agggataggc cgcggggttc atcgtgttcc agaaggtcgc
     4441 gggctcgcgg ccgacgaatt cgatgaactc gaccgacttg atcgacttgt agccatactt
     4501 ccagggcacg accagccgca agggcgcgcc gtgctgtttg aggagcggcc gaccataaac
     4561 tccaaccgcc aagagcggca gctcgttggt ggcttcggcg agcgtcaggc cctcggtgta
     4621 cggccagggg aattcgccgc cttccgcctg gtgcggcgct tcgctcggcc gctggaacga
     4681 gaccagccgc acgtgcgtcg cctgggccag cggatcggcc gccttgacca gcgccgccag
     4741 cggaatgccg gtccagggaa tcgccatcgc ccaccgctcg acgcagcggt ggcgatactg
     4801 ccgctcgacg agctcgctct ggaatagctt gtgaaacgcg tccagatcga gcgtcagcgg
     4861 attgcggcag agcccgccga tggtgatcgt ccaagcttcg ggccgaaatc gctggacgta
     4921 cttccagcac cacttgaaac ggctgaattc gtaaaagttt gtgtagcggg cggcttcggc
     4981 ggcgatcgtt tccgcccggc catacgtgaa tcgctcgtcg cgccgcgccg ggtagaaact
     5041 ggcatacttc tgctccgact ccggcgacca gcggccggct gcgatcactt cgtcatcctg
     5101 cccgttggcg ctccgccgcc acatcgcata gccggcgcct cccacgatcg ccgcaccgcc
     5161 ataggcggcg aggcgcagcc agctccgccg gttaaggaag accctttccg gtgtttcctg
     5221 cccgccgcga ggggcgaggg gatcgggctg gtgaacgaac attttgcctg gccaggattt
     5281 gacagcgccg tccgcacggc agcgccggcg gcacagccgc tacatcttac cgcccgccgg
     5341 ctccagcggc gcgaaggctg tgaaaacgta gcacttcgcg aaagtcgtgc gaatgttccg
     5401 ctcgattggc gggacacgga aaacgtggct attccggcgc ggtcggccga tgcaagttat
     5461 gcccacccgg cagtccttgc ccaggattgc cacccaccgc ccgacgcgca gcggcccccc
     5521 tcaaccgcga atagccggcg ttcccccgcc gcgccagcgc tcgggcccaa tccccgccac
     5581 aggctcagcc gtcccccgcg gctgagcctt ttttgttagt gctagggcta gagtgtaggg
     5641 ctagggtggt gccgctgaat ccctagcacg acccactagc cctagccctt cctattccca
     5701 ctcgatcgtg gccggcggct tgctgctgat gtcgtagcag acgcggttga cgcctttgac
     5761 ttcgttgatg atccgcgtgc tgatgcgggc cagcacatcg tagggcagat gcgaccagtc
     5821 ggcggtcatg aagtcgtcgg tgttgacgca ccgcaaggcg accgcgttct cataggtccg
     5881 ggcgtcgccc atcacgccga cgctttgcac gggcagcagc acggcgaagg cttgcgaggt
     5941 ctggcgatag agcccggcgg ccttgatctc ctcgatcatg atcgcgtcgg cttcgcgaag
     6001 gacgtccaac cgctgcttgg tcacttcgcc caggcagcgg accgccaggc cgggccccgg
     6061 gaagggatgc cgccagacga ggtcctcggg caggcccagc tccagaccca ggcggcgaac
     6121 ttcgtccttg aacaggtcgc ggagcggctc gaccagctcg aagccgagct ccgccggcag
     6181 cccgccgacg ttgtggtgca gcttgatcgt cgccgccggg ccgtcggccg ccgcgccgct
     6241 ttcgatcacg tcgggataga gcgtgccctg ggccaggaac ttcacgccct ggatctgctt
     6301 cgcttcttcc ttgaagcact cgatgaaggc gtggccgatc cgccgccgct tttcctgcgg
     6361 ctcggtgatg cctttcaaat cggcgaggaa ccgcggctcg ccctggacga cgtgcaggtc
     6421 ggtcttgaag tgccgggtga attcggcgat caccgccgcc tgctcgtctt ttcgcaagag
     6481 accgttatcg accaggatgc aggacagctg cgagccgatc gcctgataga gcagcgccgc
     6541 cgtaacggcc gaatcgacgc cgcccgacag gccgcagatg acgcggtgct ggccgatccg
     6601 ctcccgcatc gcccggatcg tctccttggc aaagtcgccc agcttccagg tgccggtgca
     6661 gccgcagacg gtcatcagga agttgtgcag caggtggctg ccgatggcgg tgtgcgtgac
     6721 ctcgggatgg aattgcacgc agtagatcgg cagcgacttg tgcttgaccg cggcgatcgg
     6781 gcaggtgccg gtcttggcca gcggcgtgaa gtcgtccgag acgcgcatca cctggtcgcc
     6841 gtggctcatc cacacgtcga actgctcagg aacgcccgcg aacaagtcgc tcggctgtgt
     6901 gacgcggcag cgggcgcggc cgaattcgcg ggccggaaag ctctcgacct tgccgcccag
     6961 cacctcgcag gccaggtgca tgccgtagca gatgccgagc accggaatgc ccaggcgaaa
     7021 gatcgccggg tcgcacttcg gcgctccggc ttcatagacg ctcgccggcc cgccgctgag
     7081 gatcagcccc cgcgggttgt ggcggcgaat ctgctcgggc gacagatcgt ggcggacgat
     7141 ctcgcaatag acgcgattct cgcggacgcg gcgggcgatg agctgggcga actgcgagcc
     7201 aaaatcgagc accagcacgc gttcctggct ggcagcagtg gaagcggtca tggtcgatcc
     7261 ttcaacaaaa aatgccgcgg caggggggcc acggcgaatc tcggattgta ctgcaaaccg
     7321 gcccgcctgc ggacgggggc aaggtcgcgg ttttactggc gaatacgcag gcaatggccg
     7381 actcgaagaa tccgccccgc cgctggctgt ggtacgccct ggctggcggt ttccttatcg
     7441 tcgtcatcag ctgcaccggc ctgatcggct cctaccgcat ctggcggacc cggcagcagg
     7501 cgcagcagcg cgaggccgtt accgccgtgt tagcgctggg cggcacggcg caacagacgc
     7561 tgagcagcac gtcgccgatc agcgtactga tggagcaggg tgacgcgccg aaccacatca
     7621 ccctcaacga actggcgctg gaggacgacg atttgagact ttttgggtct gcgccgacga
     7681 ccaggggcct gttcctgttc aagaacgaga tcaccgacca ggggctggta cacctgcgtg
     7741 acctgaaaag tctggagacg ctcgacctgc gcagaaacac ccgcatcacc gacgccggcc
     7801 tcgtgcacct ggaaggcctg acaaacctca agcacctcta cctcatcggc accggcgtca
     7861 cgcccgccgg cgtggcgaag ctgcagaaga aactaccgaa aacgaagatc gcctactgag
     7921 ggcgagattc tcaggagggc gaagctccgc tgagccatcg cgtcccacta agcttcgcat
     7981 tcggcccggc cggcgttttg gtattctgtt catgtctgtg tccgcctccg gcca
//
LOCUS       DASRJB010000173         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1175745, whole genome shotgun sequence.
ACCESSION   DASRJB010000173 DASRJB010000000
VERSION     DASRJB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175745"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFB80_04845"
     CDS             <1..814
                     /locus_tag="VFB80_04845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN33119.1"
                     /translation="GGAGGLGRAIARRLAQEGAHLHLVDIDRAALGQAADEIRSHGVT
                     CETSVCDLADAAAIDRLADGLIARDRPLDILVNNAGVAWYGPTAKMPSEQWDRLLAIN
                     LQAPIRLTTRLLPVLLARPEAHVVNMASIAGWVCGGRYAAYHVSKFGMVGFSEALRAE
                     YCRQGLGVTALCPGPVLTSLYKNAPSGNAGRQTPAPPAWICTTPERVAAAAVRAIYRN
                     QAIRFVGLSAHLLHYTKRLAPWVFYFLHGIGRQKNLRRKLERTEANERRRAA"
     gene            928..1893
                     /gene="ricT"
                     /locus_tag="VFB80_04850"
     CDS             928..1893
                     /gene="ricT"
                     /locus_tag="VFB80_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HZN33120.1"
                     /translation="MPRYILRYGSTRAVGVFSPRGQDRYARGMRVIARTPRGLEAGDV
                     LAEASDEQAQKMSGSPGGQIVREMSAEDANELTHIKQKEQAAFETCRQHVARLGLPMQ
                     LIEIEHVFGGERLVIYYLSEERVDFRELVKGLASEFQTRIEMRQIGVRDEAKLLADYG
                     DCGQPVCCGTFLSEMPPVSMKMAKVQKATLDPTKISGRCGRLKCCLRYEYDTYEELQQ
                     ELPPIGSDVVTKNGRARVLAQEILAQQLMVAMEDNRRVLIPASDVVTVLKAGSGRRPP
                     REERGPREDRPPRGERPPRDAAPPPDAPPPDAAPPPDSPESPPES"
     gene            complement(1904..>2723)
                     /locus_tag="VFB80_04855"
     CDS             complement(1904..>2723)
                     /locus_tag="VFB80_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33121.1"
                     /translation="KMLHSLAAHGDWTFSIVFHPNGKWLATGGGDNLVKLWDWETWTE
                     IGRFTGHTDDVHAIGFTPDGQTLVSTGDDRTVRLWDVATRRERHRLAGHERTIPGLAI
                     SPDGRLAASASRDRTVRLWDIASGEAVGILEGHTADVMSVAFHPHGRELASASYDGTL
                     ILWSIADRQPRAKLAGHGDWAFGVRYSPDGQTLVSTAGDGVRAWDSQTGRLLWHSAAQ
                     RNVSTALWLSNTEFATSSADSSIAIWNTASPSELARLWPRFTDEALARRVQVAE"
BASE COUNT          452 a          914 c          952 g          405 t
ORIGIN      
        1 cggcggcgcg ggcggcctgg ggcgggcgat cgcgcggcgg ctggcccagg aaggagcgca
       61 cctgcacctg gtcgatatcg accgcgcggc actggggcaa gcggcggacg agattcgcag
      121 ccacggcgtc acctgcgaaa cgtcggtctg cgatctggcc gacgccgcgg cgatcgaccg
      181 gctggcggat ggactgatcg cccgcgatcg gccgctcgac atcctggtga acaacgcggg
      241 cgtcgcctgg tatggaccga cggccaagat gccgagcgag caatgggaca ggctcctggc
      301 gatcaacctg caggcgccga tccggctgac gaccagactg ctgccggtgc ttctcgcgcg
      361 gcccgaagcg cacgtcgtga acatggcgag catcgcgggc tgggtctgcg gcggcaggta
      421 cgccgcgtat cacgtcagca agtttggcat ggtgggcttc agcgaggccc tgcgggcgga
      481 gtactgccgg cagggattgg gcgtgacggc cctctgcccc gggccggttc tgacgagcct
      541 ctacaagaat gcccccagcg gcaatgccgg ccgccagacg cccgcgccgc ccgcctggat
      601 ctgcacgacg cccgagcggg tcgccgcggc ggcggtccgc gcgatctatc gcaatcaggc
      661 catccggttc gtcggcctct cggcccacct gctgcattac accaagcggc tcgcgccctg
      721 ggtcttctat ttcctccacg gcatcggccg ccagaagaac ctgcgccgca agctggaacg
      781 aaccgaggct aacgagcggc ggcgggcggc ttgaaagcaa aaatcgaaga accaaagaac
      841 cgaagaacca aaggggacgg caatctggta atctggttct tggttctttg gatcttcggt
      901 tctttggttc tttggatttc ccgccccgtg cctcgctaca tcctccgtta cggttcgacc
      961 cgcgccgtgg gcgtgttctc gccccgcgga caggaccgct acgcgcgggg gatgcgggtg
     1021 atcgcccgca cgccgcgggg gctggaagcg ggcgacgtgc tggccgaggc cagcgacgag
     1081 caggcccaga aaatgagcgg ctcgcccggc ggccagatcg tccgcgaaat gtccgccgaa
     1141 gacgccaacg agctgacgca tatcaagcag aaggagcagg cggcgttcga gacctgccgc
     1201 cagcacgtcg cccgcctcgg cctgccgatg cagctgatcg agatcgagca cgtcttcggc
     1261 ggcgagcggc tggtgatcta ttacctgtcg gaagagcggg tcgattttcg cgagctggtg
     1321 aaaggcctgg cctcggagtt tcagacgcgg atcgaaatgc ggcagatcgg cgttcgcgac
     1381 gaggccaagc tgctggccga ctacggcgac tgcggtcagc cggtctgctg cggcacgttc
     1441 ctgagcgaga tgccgccggt gtcgatgaag atggccaagg tgcagaaggc cacgctcgac
     1501 ccgacgaaga tttccggccg ctgcgggcgg ctaaagtgct gcctgcggta cgagtacgac
     1561 acctacgagg agctgcagca ggagctgccg ccgatcggct ccgacgtcgt caccaagaat
     1621 ggccgggccc gcgtgctggc gcaggagatt ctcgcgcagc agttgatggt cgccatggaa
     1681 gacaatcgcc gcgtgctgat cccggccagc gacgtggtga cggtgctgaa ggccggttcg
     1741 ggccgccggc cgccgcggga agagcgcggc ccgcgtgaag accgcccgcc gcgcggcgag
     1801 cgcccgccgc gcgatgcagc gccgccgccc gatgcaccgc cgcccgatgc agcgccgccg
     1861 cccgattcgc cggaatcgcc tcccgagtct tgacgcgcga tcgctactcg gcgacttgca
     1921 cgcggcgggc gagggcctcg tcggtgaatc gcggccagag ccgggccagc tcgctggggc
     1981 tggcggtgtt ccagatcgcg atgctgctgt cggccgacga agtggcgaac tccgtgttgc
     2041 tcaaccacag cgccgtggat acgttccgct gcgcggcgga gtgccagagc agacgcccgg
     2101 tctggctgtc ccaggcgcgg acgccgtcgc ctgccgtcga gacgagcgtc tggccatccg
     2161 gcgaatagcg cacgccaaag gcccaatcgc cgtgaccggc cagtttcgcc cgcggctgcc
     2221 ggtcggcaat gctccagagg atcagcgtgc cgtcgtagct ggccgaagcc agctcgcggc
     2281 catggggatg aaacgcaacc gacatcacat cggccgtatg accctccaaa atgccgaccg
     2341 cctcgccgct ggcaatatcc cacaaccgca cggtgcggtc gcgcgaggcg gaggcagcga
     2401 gccggccgtc cgggctgatc gccaggccgg gaatcgtccg ctcgtggccg gccaggcgat
     2461 gccgctcgcg ccgcgtggcg acgtcccaga ggcggaccgt ccggtcgtcg cccgtggaaa
     2521 caagcgtctg gccatctggc gtgaagccga tcgcgtggac gtcgtcggtg tgccccgtga
     2581 agcggccaat ctcggtccag gtttcccagt cccagagctt gaccaggttg tcgccgccgc
     2641 cggtcgccag ccacttccca ttcggatgaa agacgatcga aaacgtccag tcgccgtgcg
     2701 ccgccagcga gtgcagcatc ttg
//
LOCUS       DASRJB010000174         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3432270, whole genome shotgun sequence.
ACCESSION   DASRJB010000174 DASRJB010000000
VERSION     DASRJB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3432270"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFB80_04860"
     CDS             <1..712
                     /locus_tag="VFB80_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33122.1"
                     /translation="LAKTFRDDWQTGTIDKQATHDLLATIYGGSADELCSQVAATINR
                     GVSPQSVFDALLVGSGELLMRQPGIVGIHTLTSSNALRYAYSACSDEETRKLLLLQNA
                     AFLPLFRGAMTSRGNVGEDKIEGLEPAEIPSDSKAALDAIFADVANNKKEAARKTLAY
                     ARANPQPHDFINRARTLTFLKGRDTHDYKFSSAVLEDYLNVSPQWRDRFLATSVFNLK
                     GSGGGDNSLVQRTRAALG"
     gene            complement(1510..2655)
                     /locus_tag="VFB80_04865"
     CDS             complement(1510..2655)
                     /locus_tag="VFB80_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZN33123.1"
                     /translation="MLEPTKSTRREFLSGTSAADALADAVAGEPQPLPLPKTVGAEPG
                     SHLLSVSREAMACLFEVVFDAATGRELTEAAVAALELLEPLESQLTVYRDTSEVSRLN
                     RRAAEEAVEVEEGLFRLLERAVALSEATLSAFDITAGRLSKVWGFYRRQGQLPAPEEI
                     AAALATVGSRHLQLDRERRTARFTQPGLELNLGAIGKGYSLDRVADALAAGGLSDFLL
                     HGGNSSVLARGRRIWDLGFGIWDSSKSEIRNPKSQIPAGWLVALRHPLKPDVRLAEFV
                     LRDQALGTSGSGTQFFHHQGKRYGHILDPRSGWPAEQVLSATVIAPTAEQADALSTAL
                     YVMGLPAAGEFCRRHADISALLVTAAQRAGGIELHPLNLPADRWRRL"
BASE COUNT          540 a          921 c          930 g          495 t
ORIGIN      
        1 cctggccaag acgttccgcg acgactggca gacgggcacg atcgacaagc aggcgacgca
       61 cgatttgctg gcgacgatct atggcggcag cgcggacgag ctctgctcgc aggtcgcggc
      121 cacgatcaac cgcggcgtct cgccgcaatc ggtgttcgac gcgctgctgg tcggctcggg
      181 cgagctgctc atgcggcagc cgggcatcgt cggcatccac acgctgacca gcagcaacgc
      241 gctgcgctac gcctatagcg cctgcagcga cgaagagacg cggaagctgc tgctgctaca
      301 gaacgcggcg ttcctgccgc tcttccgcgg cgcgatgacc agccgcggca acgtgggcga
      361 ggacaaaatc gaggggctgg aaccggccga aattccgtcc gattcgaagg ccgctctgga
      421 cgcgattttc gccgacgtgg ccaacaacaa gaaggaagcg gcccggaaaa cgctggccta
      481 cgcgcgggcc aatccgcagc cgcacgactt catcaaccgg gcccgcacgc tgacgttcct
      541 gaagggccgc gacacgcacg actacaagtt cagctcggcc gtgctggagg actatctcaa
      601 cgtctcgccc cagtggcgcg accggttcct ggccaccagc gtgttcaacc tcaagggctc
      661 cggcggcggc gacaattcgc tcgtgcagcg aacccgcgcg gcgctgggct agggtagtag
      721 aggcggctgc aggccgcggg cgaatcgcgc ggaatggacc agcctagtcc attccggcga
      781 gcggcgcgaa aaaaagttgc gcaggcgggc agagtcggaa gtgagtgcgg gcattgaact
      841 tgcgccgggg ccgctcgacg taagtggaca tgttgtgaac aaaatttgca ctagttgctt
      901 gcaatcgccg gccgtatgtg cataattgtg cgcacttgcg cccggcgtgc actctgcgga
      961 gcaccggtac gaacagcgat tcgcaatcgg ctccgctgga tctttgggaa gtcgaactta
     1021 gctggcgtcg cggccccagc gcgcggcgcg ataacgcggc catttttcac cacggtggtg
     1081 aaaacctaaa gccaatcgaa gccagcagtt atggcgatcg gcgcgagtta ttcaccagat
     1141 ccccaggtag cgggccccca tggtgaatta ttcggcgact tttgcaagcg ccgcccaagc
     1201 tgaaagtcgt gcagaggccg gaacttatgc cggcgatgcc cgtcgtcagt cggtcgatta
     1261 attcaccaca gagcctgccc tgcagacccg gtgagaaatg atgcttggga ggcggcggac
     1321 tcttgaacag aaacccccat gatcgcgttt tttggtcagt aactcggagc tgtcgcaaca
     1381 gaccgaattc gctcaacgtc attgaccgca acgacttgcc gccaaagcgt cgcaggaatc
     1441 gggccgaatt cttgaacacg acgcgcttcg cttttcgtga tcaacaattt gcccaaccgg
     1501 ctgaggccgt catagtcgcc gccagcggtc ggcgggcagg ttgagcgggt gcaactcgat
     1561 gcccccggcc cgctgggccg cggtgacgag cagcgcggag atgtcggcgt gccgccggca
     1621 gaactcgccg gcggccggca ggcccatgac gtagagcgcc gttgaaagcg cgtcggcctg
     1681 ctcggcggtc ggcgcgatga ccgtggccga gagcacctgc tccgccggcc agccgctgcg
     1741 cggatcgagg atgtgcccgt accgctttcc ctggtggtga aagaactgcg tgcccgagcc
     1801 gctcgtccct agggcctgat cgcggagcac aaactccgcc agccgcacgt ccggcttgag
     1861 cggatggcgg agggcgacga gccagccggc gggaatttgg gatttgggat ttcgaatttc
     1921 ggatttggat gaatcccaaa tcccaaatcc caaatcccaa atccgccgcc ctcgggccag
     1981 cacgctgctg ttgccgccgt gcagcaaaaa gtcggagagg ccgccggcgg ccagcgcatc
     2041 ggccacgcgg tcgaggctgt atcccttgcc gatcgcgccg aggttcagct ccagtcccgg
     2101 ctgcgtgaat cgcgcggtgc gccgctcccg atcgagctgc aaatgccggc tgccgacggt
     2161 ggccagcgcc gcagcgattt cctccggcgc aggcagctgg ccctggcggc ggtagaagcc
     2221 ccagactttg ctcaatcggc cggcagtgat gtcgaaagcg ctaagcgttg cctccgatag
     2281 ggcgaccgcc cgctcgagga gccgaaacag gccttcttcg acttccacgg cctcttcggc
     2341 cgcccggcgg ttgagccggc tgacctcgct cgtgtcgcga tagaccgtga gctgggattc
     2401 cagcggctcg agcaattcca gcgccgccac cgccgcctcc gtaagttcgc ggccggtcgc
     2461 ggcatcgaag acgacctcga acaggcaggc catcgcttcg cggctgaccg agagcagatg
     2521 cgaccctggt tcggccccca cggtcttggg gagcggcaac ggctgcggct cgccagccac
     2581 cgcgtccgcg agcgcatcgg cggcgctcgt gccgctcaaa aactcgcggc gggtcgattt
     2641 cgttggttcg agcatcaaag tggagccaac tagggcggta gaacgttatt atattgtgaa
     2701 tagtcaccgc tgcccgccgg ctatgaacag aaggaaacga agataacgaa gcaggcaata
     2761 caatttgatc tttcttcgat tccttcgtta ccttctgttc gcctgcctgt ccgcccgccg
     2821 ggaactcgac gatgcgacga ttcgctgtgt tgctgctgat tgttctcgcc gttccagcgc
     2881 tggcgg
//
LOCUS       DASRJB010000175         5004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2017495, whole genome shotgun sequence.
ACCESSION   DASRJB010000175 DASRJB010000000
VERSION     DASRJB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017495"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFB80_04870"
     CDS             <1..857
                     /locus_tag="VFB80_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33124.1"
                     /translation="AVVEPSRTRFLDFVKSIKFSAGTEPRPTWTLPADWEELPGNQIR
                     LATLRFQSGGEPLEITVIPAGGTVLDNVNRWRDQVNLEPIPADELASTTESFKVDGRP
                     CTFASLVGTRSSGGGPMGSAPFAPFAGGATSAPPPVSPKSAGPPAGGDLKYDAPKEWS
                     TAPNNAVSVAAFEVTDGKQRVEITVSPVGGDLLSNVNRWRDQVGLPPIDIGELAKSLQ
                     KIATLGATGDYVEAVGPGDVTAAQTILGVRVDAGGGTWFIKLRGDRDLAAREKAHFEA
                     FVKSLQFK"
     gene            894..4625
                     /gene="ccsA"
                     /locus_tag="VFB80_04875"
     CDS             894..4625
                     /gene="ccsA"
                     /locus_tag="VFB80_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZN33125.1"
                     /translation="MSAPAVPVAEFPRPLASDDALSIASLINGAMKGLASLKLTVALF
                     VAGIFVVYVGTVAQQQADIWQVVRDYFHAWVMWVDVNLLFPKAFFPFLPEMPIPPLPA
                     PGGLSVGLLMAINLLAAHGWRFTIQAKGMRLVLGIFVSLIGLVVGAAIIVAGHNAEGF
                     QAKPPFSWDVFWTVFLTTSALLVVASIAAYVYFEVMVVLNKPSIPWVEIVMLVLVGHA
                     FAGIGGLLCWGFFAEKRPSDEALRIVWQLVQGGLAGLVLLAGCILVFRKRGGMVLLHV
                     GIGLLMFNELWVAMNNRERQVFMMEGQTVNYLRDTRTVELAIIDRSDQKVDEHVVVPR
                     QLLESNFRANQKLQRDGKPPVFLDNPMLPVKLAVLEYYKNASVRRLKAGERTLATIGR
                     GLKEALVELPIAKGTDSDSAVDIAGAYVRFADKASGKDLGTFLLSQRAELEQKPHLFA
                     EKLDAGKQQFDLFLRFQREYKPYLLSLVDVRKDDYVASNTPRNYSSDVRLNDNQAAVD
                     QTVHIKMNDPLRYRGDTFYQSGYHPPEQTGGVEATTLQVVHNRGWMIPYVACMIVVIG
                     MIAHFLITVTRFVGRREVEEEAAGEVITAQLVDAPQAAHPAGKNPARGKRKAERIGFD
                     WSLVGLPLLAAGILLAWMSYAVRPPKPQAQGMDIVRFGQLPVGHDGRVKPLDTLARNM
                     LRAISLRETVKVGSERLSATQWLLEVISRTPRADTLRVIRIDHPDVLKLFNLPRRAGF
                     VYSVDEIRSGMDDFLAQLAAMTKAEEEGRASKDDRTAFQRKLIELHERLQIYIILTES
                     FELPPMPNLEELQKDKALAEQYFSNLRAYLDRVEGELNRSRAPKPVPVAIQAGQPAES
                     AWQHMPTAWRLGLVSGEPDQVVDAYQKMKAAYHDGDAGAFNAALVRYESSLARSPPPL
                     YEARRVGLEAYFNHVSPFYAGITFYVLAFLLAVLGWLLQYRPLNWTAFTVLLLTFLFH
                     TAALVLRIYISGRPPVTNLYSSAIFIGWAVVLAALCLELIFRLGLGNVAGSIAGFATL
                     LISYFLSTGGDTITVLQAVLDTQFWLATHVVCVTLGYSATYLAGIFGLLYVIFGLTTP
                     LLNERSRKDLGRMIYGITCFAILFSFYGTVLGGLWADDSWGRFWGWDPKENGALLIVL
                     WNALILHARWDKLVGDRGLAVLAIGGNIITTWSWFGVNLLGIGLHAYGGFNGSAPLIV
                     LVVVAALLVLMGLGCLPKDWWWSFRADTPTRGPMLKPAG"
     gene            4820..>5004
                     /locus_tag="VFB80_04880"
     CDS             4820..>5004
                     /locus_tag="VFB80_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33126.1"
                     /translation="MGLHFAIGRRDGKTLFHQAATANLSSRSNGRRAMPSLPHARPAE
                     LGLDEHRLQSAYDRLDE"
BASE COUNT          819 a         1686 c         1600 g          899 t
ORIGIN      
        1 cggccgttgt tgagcccagc cgcaccaggt ttctggactt tgtgaagtcg atcaagttct
       61 ctgccggcac ggagccgcgg ccgacctgga cgctgcccgc cgactgggaa gaactgcccg
      121 gcaatcaaat tcggctggcg accttgcggt ttcaatcggg cggtgagccg ctggagatca
      181 ccgtgattcc cgcaggcggc acggtgctgg acaacgtcaa ccgctggcgc gaccaggtga
      241 acctggagcc gattcccgcc gacgagctgg cgagcacgac cgagagtttt aaggtggatg
      301 gccgcccgtg cacgttcgcg agcctcgtcg gcacgcgctc tagcggcggc ggcccgatgg
      361 gctcggcccc gtttgctccc ttcgcggggg gcgcgacgag cgcgccgcct ccggtgtcgc
      421 caaaatcggc gggcccgcca gccggcggcg atttgaagta cgacgcaccg aaggaatgga
      481 gcaccgcacc gaacaacgcg gtgagcgtgg cggcctttga agtgaccgac ggcaagcagc
      541 gcgtggagat tacggtcagt cccgtcggcg gcgacttgct ttccaacgtg aatcgctggc
      601 gcgatcaggt cggcctgccg cccatcgaca tcggcgagct ggccaagagc ctccagaaaa
      661 tcgcgacgct gggggccacc ggcgattacg tcgaagcggt cggccccggc gacgtcaccg
      721 cggcccagac gatcctcggc gtgcgggtcg atgccggcgg cgggacgtgg tttatcaagc
      781 tccgcggcga ccgcgacctg gccgcccgcg aaaaggccca cttcgaagcc tttgtgaagt
      841 cattgcagtt caagtaacca gcgcgaccac tggtcgcggc tttgagaatc gccatgtccg
      901 ctcctgccgt tcccgtcgcc gaattccccc gaccgctggc ctcggacgat gccctctcaa
      961 tcgccagcct catcaacggg gcgatgaagg gcctggcttc gctcaagctg accgtggccc
     1021 tgttcgtcgc cggcattttt gtcgtctacg tcggcacggt cgcccagcag caggccgaca
     1081 tctggcaggt cgtccgcgac tacttccacg cctgggtgat gtgggtcgat gtcaacctgc
     1141 tcttccccaa ggcgttcttc ccgtttctgc cggaaatgcc gatcccgccg ctgcccgcgc
     1201 ccggcggtct ttccgtcggc ctgctgatgg cgatcaactt gctcgccgcc cacggctggc
     1261 gattcacgat ccaggccaaa gggatgcggc tggtgctggg cattttcgtt tcgctcattg
     1321 gcctcgtggt cggcgctgcc atcatcgtag ccggccacaa cgccgaaggc tttcaggcca
     1381 agccgccgtt ctcgtgggac gtgttctgga ccgtatttct caccaccagc gccctcttgg
     1441 tcgtagcgag catcgccgct tacgtctatt tcgaagtgat ggtggtcctc aacaagccga
     1501 gcatcccctg ggtcgagatc gtgatgctgg tgctcgtggg acacgctttc gccgggatcg
     1561 gcggattgct ttgctggggg ttttttgccg agaagcgccc cagcgacgaa gccctccgca
     1621 tcgtctggca actcgtgcag ggcggcctgg cgggactggt cctgctcgcc ggctgcatcc
     1681 tcgtgttccg caagcggggc gggatggtgc tgctccacgt gggcattggc ctgttgatgt
     1741 tcaacgagct gtgggtggcg atgaacaacc gcgagcggca ggtcttcatg atggaaggcc
     1801 agaccgtcaa ctacctccgc gacacgcgga cggtcgagct ggcgatcatc gaccgctcgg
     1861 accagaaggt cgatgagcac gtcgtcgtgc cgcggcagct cttggaatcg aacttccgcg
     1921 cgaaccagaa gctgcagcgc gacggcaagc cgccggtctt tctcgataac cccatgctgc
     1981 cggtcaagct ggccgtcctg gagtactaca agaacgccag cgtccggcgc ttaaaggcgg
     2041 gcgagagaac cctcgcgacg atcggccgcg gcctgaagga ggcgctcgtc gagttgccga
     2101 tcgccaaggg caccgacagc gattcggccg tcgatatcgc cggggcttac gtgcggtttg
     2161 cagacaaggc gagcggcaag gacctgggca cttttctgct ctcgcaacgg gccgagctgg
     2221 aacaaaagcc gcacctcttt gccgagaagc tcgacgccgg caaacagcag ttcgacctgt
     2281 tcctccgctt tcagcgcgag tacaagccct acctgctcag cctcgtcgat gtgcggaagg
     2341 acgactacgt cgcctcgaac acgccgcgga actactcttc cgacgtccgc ctgaacgaca
     2401 accaagcggc cgtcgatcag accgtgcaca tcaagatgaa cgacccgctg cggtaccgcg
     2461 gcgacacgtt ctatcaaagc ggctatcacc cgcccgaaca gaccggcggc gtcgaagcca
     2521 cgacgctgca ggtcgtgcac aaccgcggct ggatgattcc ctacgtcgcc tgcatgatcg
     2581 tggtgatcgg aatgatcgcc cacttcctga tcacggtcac gcggttcgtc ggccgccgcg
     2641 aggtggaaga agaagccgcc ggtgaggtca tcaccgcgca gctggtagat gcaccgcaag
     2701 ccgcgcaccc tgccggcaaa aacccggccc gcggcaagcg caaggccgag cgcattggct
     2761 tcgactggtc gctcgtcggc ctgccgctct tggccgccgg catcctgctg gcctggatgt
     2821 cgtacgccgt ccgcccgccc aagccgcagg cccaaggaat ggacatcgtc cgcttcggtc
     2881 aattgcccgt cgggcacgac ggccgcgtca agccgctcga cacgctcgcc cgcaacatgc
     2941 tgcgggccat ctcgctccgc gaaacggtca aggtggggag cgagcggctg agcgcgacgc
     3001 agtggctgct ggaggtcatt tcgcggacgc cccgggccga tacgctccgc gtgatccgca
     3061 tcgaccatcc cgacgtgctg aagctattca acctgccgcg ccgcgcgggt ttcgtgtaca
     3121 gcgtcgatga aatccggtcg ggcatggacg acttcctcgc acaactggcc gcgatgacca
     3181 aggccgagga agagggccgc gccagcaagg acgaccgcac cgcctttcag cgcaagctga
     3241 tcgagctgca cgagcggctg cagatctaca tcatcctgac cgagtcgttc gagctgccgc
     3301 cgatgcccaa cctcgaggag ctgcagaagg ataaggccct cgccgagcag tacttcagca
     3361 acttgcgggc ctacctcgac cgcgtcgaag gcgagctcaa ccgcagccgg gcccccaagc
     3421 cggtgccggt ggcgatccag gccggccagc ccgcggaaag cgcctggcag cacatgccga
     3481 cggcctggcg gctgggcctg gtctcgggcg agcccgacca ggtcgtggac gcctaccaga
     3541 aaatgaaagc cgcctaccac gacggcgacg ccggggcgtt caacgccgcg ctggttcgct
     3601 acgagtcctc gctggcgcgc tcgccgccgc cgctctatga ggcccgtcgc gtgggcttgg
     3661 aggcgtactt caatcacgtc tcgccgttct acgcgggcat caccttctac gtgctggcgt
     3721 tcctgctggc cgtgctcggc tggctgctgc agtatcggcc gctgaactgg accgcgttca
     3781 ccgtgctgct cttgacgttc ctgttccaca ccgcggcgct ggtgctgcgg atctacatct
     3841 ccggccgccc gccggtgacc aacctctatt cctcggccat cttcatcggc tgggccgtgg
     3901 tgctggccgc gctctgtctg gagttgattt tccgcctcgg cctgggcaac gtcgccggct
     3961 cgatcgccgg gttcgccacg ctgctgatct cgtacttcct ctcgaccggc ggcgacacca
     4021 tcaccgtgct gcaggccgtg cttgacacgc agttctggct ggcgactcac gtagtgtgcg
     4081 tcacgcttgg ctattcggca acgtacctcg cgggcatatt cggcctgctc tacgtgatct
     4141 tcgggctgac gacgccgctc ctcaacgagc gctcgcgaaa ggacctgggc cggatgatct
     4201 atggcatcac ctgcttcgcg atcctgttca gcttctacgg cacggtgctc ggcgggctgt
     4261 gggccgacga ttcctggggc cgcttctggg gctgggatcc gaaggaaaat ggcgcgctat
     4321 tgatcgtgct ctggaacgcc ctgatcctgc acgcccgctg ggacaagctg gtcggcgacc
     4381 gcggcctggc ggtgctcgcg atcggcggca acatcatcac cacctggtcc tggttcggcg
     4441 tcaacctgct gggtatcggc ctgcacgcct acggcggctt caacggctcc gccccgctga
     4501 tcgtcctggt tgtcgtcgcc gccctcctcg tgttgatggg cctgggctgc ctgcccaaag
     4561 attggtggtg gagcttccgc gccgacacac ccacccgtgg gccgatgctg aagcccgctg
     4621 ggtaacgtag cggcgccatg caggcccaaa gggccgacct tcgttagccc agggcgcagc
     4681 ggcgcagccg cgagccctgg gttggcgtgc gagagattgc cagccccaac ggggcgcgat
     4741 ctcgccccga tggggctgat gacttctcaa atgtcatggc tacccaggcc gttggcctgg
     4801 gctgacgaag gtcggcccgt tgggcctgca cttcgcgatc ggccgacgag atggcaaaac
     4861 gttgtttcat caggcagcga cggctaatct gagtagtcga tccaacggga gacgcgccat
     4921 gccttcgctg ccccacgctc gccccgcgga acttggcctc gacgagcacc gcctgcagtc
     4981 ggcctacgat cgcctggacg aatg
//
LOCUS       DASRJB010000176         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3433723, whole genome shotgun sequence.
ACCESSION   DASRJB010000176 DASRJB010000000
VERSION     DASRJB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433723"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2830)
                     /locus_tag="VFB80_04885"
     CDS             complement(53..2830)
                     /locus_tag="VFB80_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN33127.1"
                     /translation="MLRVIASVSVLFLSGALWAADEAVPVSAVHEILAAKCLACHGND
                     PQDLKGKFDLRSQAAAIKGGESGEAAIVPGEPDKSPLYRAVTWEDDALQMPPKLNDRL
                     SEAQVALIRRWIAGGAKWEEPAALANSGKAWSSGPGGIRIVTSGGRSPEWDSRTYEPA
                     AVWAYQKAAGTLRVPPIEGVAHPIDAFLLAALQPKGITGFAPRADERTLLRRVTFDLT
                     GLPPSVSPSLFLSVANDRETERHRDGETYEHVIQRLLASPRYGEQQARHWLDVVRYAD
                     TAGFSNDFERPNSWRYRDYVIRSFNADKPFDRFLTEQLAGDELDPSDPELLIAAGYLR
                     MGPWEHTGMTVAAITRQQYLDDVTNHVGVSLLGQGLRCAACHDHKFDPLPTRDYYRVQ
                     AVFAPVQFAERPAAFLTSENVRNFAAAQALVQQRLDQIQAAQAALRTKNQDAIAAFLA
                     EKGVKSLADLPPEERPKQDYLGGTFGLTKTDLSLRKIYQKSQAYLERELKRFEPLALS
                     VYSGPPNTYTSLKPLYEVPRRIDGVVPVVHILPGGSLASPADEVTPGVLSAMIGSNDR
                     LAPSAWNSIPDNTAGRRLALARWIASRENTLTARVIVNRVWQQHFGTGLVATPNNFGK
                     MGSRPSHPELLDWLATWFVDNGWSLKKLHQLILTSTAYQQSCERPDLEKVRAVDAKNA
                     LLAYFPPRRLAAEEIRDAMLAASGELNLEVGGPGVFPELNWEVALQPRHIMGSVPPAY
                     VPSLTPAERNRRTIYAFRIRTLADPLLEVLNRPLSETSCERRDETTVTPQVFALFNGE
                     FAANRALAMAARLTKEHSSGAERITAAFRTVYGRQPTATEREKCLAHLAKMTAHHQAH
                     PPTATELPTKVRRGMVEELTGEMVYWDEDLSILNGYQRDLMPWQVEPAARALADVCLV
                     LMNSNEFLYLR"
     gene            complement(2880..3617)
                     /locus_tag="VFB80_04890"
     CDS             complement(2880..3617)
                     /locus_tag="VFB80_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN33128.1"
                     /translation="MPWTTETVGGHPCDVYLPPQRSPHGYVALYLHGVHQNRLHDKPK
                     FVEQFDKHGLAVVCPLTQRSWWTDKICLEFDPRISAQRHVLENVLPFIEQQLGAKPPR
                     IGLLGTSMGGQGALRLAYKFPNVFPVVAAVSPAIDFHKRYNEGDETIPQMYPDPEAAR
                     QDTAILHVHPLNWPRHQFFCCDPEDYRWWESADRLRMKLYSLGVPHQCDLTTTGGGHG
                     FGYYNRVAERAIGFLAEKLDEERRRIV"
     gene            complement(3699..4553)
                     /locus_tag="VFB80_04895"
     CDS             complement(3699..4553)
                     /locus_tag="VFB80_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZN33129.1"
                     /translation="MSSTLRTLLAEGLGTFILVVFGCGAVHSAVLTGAQSGLWQVAIV
                     WGSGVAFAAYCVGGVSGAHINPAMTIALAVWGRFPWSKVGPYIAVQMAGAFLAAAALF
                     VIYGPSLKAKEDAIGVKRGEPGSIVTASCYGEFYPNPGGALTAEGKWSDSHWQVFQAR
                     ASFREAFVAEVLCTAILALVVFAATDGRNSAAPANFLAPLVIGLTVACLISIIAPLTQ
                     CCLNPARDFGPRLFTYLAGWGSAALPGPNGHGFFTVYILAPILGAIAGGGIYDGLLRP
                     AFGEQKKG"
     gene            4668..4985
                     /locus_tag="VFB80_04900"
     CDS             4668..4985
                     /locus_tag="VFB80_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN33130.1"
                     /translation="MATKDPLSSKECAERLKALADADRLRIVQVLRGGPQNVSDISAA
                     LSADIANVSHHLQILKREKIVETEKQGRFVVYRLHPDVFAAEKATASDCLDLGCCKLE
                     LPK"
     gene            complement(4995..>5366)
                     /locus_tag="VFB80_04905"
     CDS             complement(4995..>5366)
                     /locus_tag="VFB80_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZN33131.1"
                     /translation="CAHACELETSLYLYLDGDNVRKDLIKNGTISFNEDDSPFMWVDL
                     FGAGPATVISWTSSYSETGVLGDAELATAEKGREAYEEAVKQLCRFVTWWKDRPKDQR
                     RDRHRRPPTIPIPWGQRPLER"
BASE COUNT          943 a         1713 c         1782 g          928 t
ORIGIN      
        1 gagtaccgag tacttggtgc tttgcctgtc gtacgtcgca cgccatacgt ccctaccgca
       61 aatacaggaa ctcgttcgaa ttcatcagca cgaggcacac gtccgccagc gcccgcgcag
      121 ccggctcgac ctgccacggc atcagatcgc gctggtagcc attgaggatc gagaggtctt
      181 cgtcccaata gaccatttcg ccggtcagct cctcgaccat gccacggcgg actttcgtcg
      241 gcagctcggt cgccgttggc ggatgggcct ggtggtgcgc ggtcatcttg gccagatggg
      301 ccaggcactt ctcgcgctcg gtagcagtcg gctggcggcc gtagaccgtg cgaaacgcgg
      361 cggtgattcg ctctgcgccc gaggaatgct ccttcgtcag gcgggccgcc atcgccaacg
      421 cccggttggc ggcgaattcg ccgttgaaca gggcaaagac ctgtggcgtg acggtcgtct
      481 cgtcgcggcg ttcgcagctc gtctcgctca acggccggtt gagcacctcc agcagcgggt
      541 cggccagcgt gcggatgcgg aaggcataga tcgtccggcg attgcgttca gccggcgtga
      601 gcgaggggac ataagccggc ggcaccgagc ccataatgtg ccgcggctgg agagccacct
      661 cccagttcag ctcaggaaag acgccggggc cgcccacttc caagttcagc tcgccgctgg
      721 cagccagcat cgcgtcccga atctcttcgg cggccaatcg ccgcggcggg aagtatgcca
      781 gcagcgcgtt cttcgcatcg acggctcgga ccttttccag gtcgggccgc tcgcaggatt
      841 gctggtaggc cgtgctcgtc agaatcagct ggtgcagttt cttgagggac cagccgttgt
      901 ccacaaacca ggtggcgagc cagtcgagca gctccggatg gctgggccgg gagcccatct
      961 tgccaaaatt gttcggcgtg gcgacgaggc ccgttccaaa atgttgctgc cagacgcggt
     1021 tgacgatcac ccgggccgtc agcgtattct cgcggctggc aatccagcgg gccagagcca
     1081 accggcgtcc agccgtatta tccgggatgg aattccaagc ggaaggcgcg aggcggtcgt
     1141 tggaaccgat cattgcgctg agcacgccag gtgtcacttc gtcggccggc gacgccagtg
     1201 agccgccggg cagaatgtgg acgaccggca cgacgccgtc tatccgccgc ggcacctcat
     1261 acagcggctt gagcgacgtg tacgtattgg gcggccctga atagacgctg agagctagcg
     1321 gctcgaaccg cttcagctcg cgttccaggt aggcctgcga cttctggtag atcttccgca
     1381 gcgagaggtc cgtcttcgtg aggccgaatg tgccgccgag gtagtcctgc ttcggccgct
     1441 cttcgggcgg caggtcggcc agcgatttca cgcccttttc ggcgaggaat gctgcaatcg
     1501 cgtcctggtt cttcgttcgc agggcggcct gcgccgcctg aatctggtct agacgctgct
     1561 ggaccagggc ctgggccgcg gcgaagttcc gcacgttctc gctcgtgaga aacgccgcgg
     1621 gccgctcggc gaattgcacc ggcgcgaaga ccgcctgcac gcggtagtag tcgcgggtcg
     1681 gcagcggatc gaacttgtgg tcgtggcagg cggcacaccg caagccctgg ccgagcagcg
     1741 acacgcccac gtggttcgtc acatcgtcga ggtactgctg ccgcgtgatg gcggcgaccg
     1801 tcatcccggt gtgctcccac ggtcccatcc gcaggtagcc ggcggcgatc agtaattccg
     1861 ggtcgctcgg atcgagctcg tcgccggcca gttgctcggt gagaaagcga tcgaacggct
     1921 tgtcggcgtt gaagctgcgg atgacgtagt cgcggtaccg ccaggaattc ggccgctcga
     1981 aatcgttcga gaagccggcc gtgtcggcat agcggacgac gtcgagccag tgccgggcct
     2041 gctgctcgcc gtagcggggg ctggcgagga gtctctggat cacgtgttcg tatgtctctc
     2101 cgtctctgtg tctctccgtc tctctgtcgt tcgcgacgga gagaaagagc gacggagaga
     2161 cagagggagg caggccagtc aaatcgaaag ttacgcggcg gagcagggtc cgctcatccg
     2221 cgcgtggcgc gaagccggtg atgcccttgg gttgtagtgc ggcgagaaga aacgcatcga
     2281 tgggatgagc gacgccctca atcggcggca cgcggagtgt gcccgctgcc ttctggtagg
     2341 cccagacggc tgcgggctcg taggtgcgac tatcccactc gggcgagcgg ccgccgctgg
     2401 tgacgatgcg aatgcctccc gggccgctcg accaggcttt gccggaattg gcgagcgccg
     2461 ccggctcttc ccacttcgct ccaccggcga tccagcggcg aatgagtgcg acctgagcct
     2521 cgctcagccg atcgttcagc ttcggcggca tctggagcgc gtcgtcctcc caggtgaccg
     2581 cgcggtagag cgggctcttg tcgggctcgc ccggcacgat tgccgcctca ccgctctcgc
     2641 cacccttgat cgccgcggcc tgcgaccgca gatcgaactt gcccttcagg tcctgcggat
     2701 cattgccgtg acaggcgagg cacttggcgg caaggatctc gtggacggcg gaaacgggga
     2761 cagcctcgtc cgcggcccac aacgcgccgc tgaggaacag cacagaaacg gaagcaatga
     2821 ctcgcagcat cgctggtaca agggacaaat gacgactgac caatgaccaa gcttgcattc
     2881 taaacaatcc gccgccgctc ttcgtccagt ttttcggcca gaaacccgat cgcccgctca
     2941 gccacgcggt tgtagtagcc gaagccgtgg ccgccgccgg tggttgtcag gtcgcactgg
     3001 tgtgggactc cgagcgagta gagcttcatc cgcaggcggt cggcgctttc ccaccagcga
     3061 tagtcttccg ggtcgcagca gaagaattgg tgccgcggcc agttgagcgg atggacgtgc
     3121 aggatcgccg tgtcctgccg ggccgcctcc ggatcggggt acatctgcgg gatcgtctca
     3181 tccccttcgt tatagcgctt gtgaaaatcg atggccggcg aaacggcagc gaccacgggg
     3241 aagacattgg gaaacttgta ggccagccgc agcgcgcctt gcccgcccat gctcgtgccc
     3301 aaaagaccga tccgcggcgg cttcgccccg agctgctgct cgatgaacgg cagcacgttt
     3361 tccagcacat gccgctgggc cgagattctg ggatcgaact ccaaacagat cttgtcagtc
     3421 caccagctcc gctgcgtcag cggacagacc acggccaggc catgcttgtc gaactgctcg
     3481 acgaattttg gcttgtcgtg caagcggttc tggtgcacgc catgcaggta gagcgcgacg
     3541 tagccgtgcg ggctgcgctg cggcggcaga tagacatcgc acggatgtcc gccgacggtc
     3601 tcggtggtcc agggcacgag gagggctagg gctagatggt agggctaggg ccgcaagaga
     3661 gacttcaaac taaccgaccg tagccctagc ccccagtcct agcccttctt ctgctccccg
     3721 aacgccggcc tcaagaggcc gtcataaatc ccgccgccgg cgatcgcgcc gaggataggt
     3781 gcgaggatat acacggtgaa gaaaccgtgg ccgttgggac cgggcaaggc cgcacttccc
     3841 caaccggcca ggtacgtgaa cagccgcggg ccgaagtcgc gggccgggtt gaggcagcac
     3901 tgcgtgagcg gcgcgataat cgagatcagg caggcaaccg tgaggccgat caccagcgga
     3961 gcgaggaaat tcgccggggc agccgagttt ctgccgtcgg tggccgcgaa cacgaccagg
     4021 gccaggatcg ccgtgcagag gacctcggcg acaaaggcct cgcgaaacga ggcccgcgcc
     4081 tgaaagacct gccagtggct gtcggaccat ttgccctcgg cggtgagcgc gccaccggga
     4141 ttcggataga actccccata acacgatgcc gtgacgatgc tgcccggctc gccgcgcttg
     4201 acgccgatcg cgtcttcctt ggccttcagg ctcggcccat agatgacgaa tagcgccgcg
     4261 gccgcgagga acgccccggc catctggacg gcgatatacg gcccgacctt gctccagggg
     4321 aaccgccccc agactgccag cgcgatcgtc atcgccgggt tgatgtgcgc cccgctgacg
     4381 ccgccgacgc aataggccgc gaaggccacg ccgctgcccc acacgatcgc cacctgccac
     4441 aggccgctct gcgctccggt gagcacggcc gagtgcacgg ccccgcagcc gaagacgacc
     4501 aggatgaacg tgccgaggcc ttcagccagg agcgtgcgga gcgtggagga cattaggtct
     4561 gctcaagggg agagggaaag cgtatcatca acgaatcggg ctgcgattgt agccgcttgt
     4621 cgggcgctgg tatagaaatg ggggccgctg gaacctagcg aacggccatg gccacgaagg
     4681 atcccctctc atccaaggaa tgtgccgaac ggctgaaggc cctggccgat gccgaccgcc
     4741 tgcggatcgt gcaggtgctc cgcgggggac cgcaaaacgt cagcgatatc tccgcagcgc
     4801 tttcggccga cattgccaac gtctcgcacc acctgcaaat cctcaagcgc gagaagatcg
     4861 tggaaaccga gaagcagggc cgcttcgtcg tctatcgcct gcaccccgac gtttttgccg
     4921 ccgagaaggc cacggccagc gactgcctgg acctcggctg ctgcaagctc gagttgccga
     4981 agtagcgggt ctggctaacg ttccagtggc cgctggcccc aggggatcgg aatcgtcgga
     5041 gggcggcggt ggcggtcgcg gcgctggtct ttggggcggt ctttccacca ggtgacgaac
     5101 cggcagagct gcttgacggc ttcttcatag gcctcgcggc ctttttcggc ggtggccagc
     5161 tcggcgtcgc cgagcacgcc ggtctccgag tagctgctcg tccaggagat taccgtcgcc
     5221 gggccggccc cgaacagatc gacccacatg aaggggctgt cgtcttcgtt gaacgagatc
     5281 gtgccattct tgatcaggtc cttgcgcacg ttgtcgccgt cgagatagag gtacagcgac
     5341 gtttccagct cgcaggcatg ggcgca
//
LOCUS       DASRJB010000177         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3717040, whole genome shotgun sequence.
ACCESSION   DASRJB010000177 DASRJB010000000
VERSION     DASRJB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3717040"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFB80_04910"
     CDS             <1..979
                     /locus_tag="VFB80_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33132.1"
                     /translation="EIPQQHVTCYRAADGQQLWDTLVPPGPWKLTDLRGGYAAPTPVT
                     DGQRVYVVFGSSVLAALDWDGKLVWRNELPDWEAFDVAIASSPILYRGQLLLLADRNQ
                     KKSTLTAFDPATGAVLWEQKRPQGAFTHTTPVLIENRGQPQLLVTASHELQALDPASG
                     ERLWWCKTPGDVTSPVFARGLVYTDSGRGGPGLLVDATGQGDVAATHVKWRLDQIPEG
                     LSSPVIAADHLYRLHNPGVLKCVALDGGKEAFATRLAGVSVASSPVATPDGRIYFASA
                     GKSFVVKAGPAFELLATSDLGEPTSASAAFSGGRIYLKGQRHLFCIGNK"
     gene            991..1965
                     /locus_tag="VFB80_04915"
     CDS             991..1965
                     /locus_tag="VFB80_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33133.1"
                     /translation="MRGFVLACLAGSGCLCAAAPAADKQAWTPISDRVLAQVKPGYPG
                     KTAGVAVDPASGDVYMVVPDQGLWKSTDRGETFARVDGGKVGGRCETGFALQFDPAGK
                     RLMCFMIYGGSAWTADSGQSWTASKSSHLDFGGVDWGETGKCFLALRHESGGVLTLST
                     DGGQTWKDLQKGFKQLGLFSGKVLLAGRGQGIERSEDGGETWAKVSDAAPLASVMQTR
                     GGVGYWLTDQGLLISRDQGRTWRPLGPSVAAIVGPYFGASAEHLVVVGKTGFQESKDG
                     GQSWQVVAPLPDKFTVGGVGPNYAWDPHADIFYASSMGKDTLRYIRQP"
     gene            complement(1977..2321)
                     /locus_tag="VFB80_04920"
     CDS             complement(1977..2321)
                     /locus_tag="VFB80_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZN33134.1"
                     /translation="MPTYDYSCDACDHTFEVFEAISAEPQKKCPKCKKKKLRRLFGAG
                     AGLIFKGSGFYQTDYRSDSYKKAATADKPASESASPGDSSSSSDSKKSEAKSNGAAKS
                     ETAPAKKAKSGE"
     gene            complement(2431..>2976)
                     /gene="grpE"
                     /locus_tag="VFB80_04925"
     CDS             complement(2431..>2976)
                     /gene="grpE"
                     /locus_tag="VFB80_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZN33135.1"
                     /translation="EADAETMPVNRGSNGQTNADEKIARLESELREANEKALRATAEL
                     ENYRKRAQRDIADERKYAVVPLVRDMLAVVDNLERAIEATQARSASEGSANQGLLDGV
                     KMVATQLEAILKQHQCVRIETVGAAFDPNQHQAIAQEPSDQYPAGTVTRAAQSGYKLH
                     DRVIRPAQVFVSTGAPARNDN"
BASE COUNT          475 a          966 c          978 g          557 t
ORIGIN      
        1 ggagattccg cagcagcacg tcacttgcta ccgcgcggct gacgggcagc agttgtggga
       61 cacgctcgtg ccgcccgggc cttggaagct caccgatctc cgcggcggat atgccgcccc
      121 gacgcccgtc accgatgggc agcgcgtcta cgtggtcttc ggatcgtcgg tgctggcggc
      181 gctggattgg gacggcaagc tcgtgtggcg caacgagctg cccgactggg aggcgttcga
      241 cgtggccatc gcttcgagcc ccatcttgta tcgcggccag ctgcttttgc tggccgaccg
      301 caaccagaag aaatcgaccc tgaccgcctt tgacccggcc acgggcgcgg tcctgtggga
      361 gcagaagcgg ccgcaaggcg cgttcacgca cacgacgccg gtgctcatcg agaatcgcgg
      421 gcaaccacag ctgctggtca ccgcctcgca cgagctgcaa gctctcgatc cggctagcgg
      481 cgagcggctg tggtggtgca agacgccggg cgacgtcacc tcgccggtgt tcgcccgcgg
      541 cctggtctat accgacagcg ggcgcggcgg gcccggcctg ctcgtcgatg ccacgggcca
      601 gggcgacgtg gcggcgactc acgtcaaatg gcggcttgac cagatccccg agggcctgtc
      661 ctcgccggtg atcgctgccg accacctcta tcgcttgcac aacccgggcg tgctgaagtg
      721 cgtcgccctg gatggcggca aggaagcctt tgctacgcgg ctggccggag tctcggtcgc
      781 ctccagcccg gtggcgacgc cagacggaag aatctacttc gccagcgccg gcaaatcgtt
      841 cgtcgtgaaa gccgggccag cgttcgagct gctcgcgacc agcgacctgg gcgagccgac
      901 ctccgcctcg gcggcgttca gcggcggcag gatctatctc aagggccagc ggcatttgtt
      961 ctgcattggc aataaatagg agcatctgcg atgcgcggct tcgttctcgc ctgcctggcc
     1021 ggatctggct gcctgtgcgc cgctgcaccc gcggccgaca aacaagcctg gacgccgatt
     1081 tcggatcgcg tcctcgcgca ggtcaagccg ggctatcccg gcaagaccgc gggagtggcc
     1141 gtcgatcccg ccagcggcga cgtctacatg gtcgtgccgg accagggcct gtggaaaagc
     1201 accgaccgcg gcgagacttt tgcccgcgtc gatggcggca aagtcggcgg ccgctgcgag
     1261 accggcttcg ccctccaatt cgatcccgcc ggcaagcggc tgatgtgctt catgatttat
     1321 ggcggcagcg cctggacggc cgacagcggg caatcctgga ccgccagcaa gagcagccat
     1381 ctcgatttcg gcggcgtcga ttggggcgaa accggcaagt gttttctggc cctgcggcac
     1441 gaaagcggcg gcgtcctcac gctctcgacc gacggcggcc aaacctggaa ggacctccag
     1501 aagggcttca agcagctcgg cctgttcagc ggcaaggtcc tgctcgccgg tcgcgggcaa
     1561 ggcattgagc ggagcgagga cggcggcgaa acgtgggcga aggtctcgga tgcggcacct
     1621 ctggccagcg tcatgcaaac ccgcggcggc gtcggctact ggctcaccga ccagggcctg
     1681 ctcatcagtc gcgaccaggg ccggacctgg cggccgctgg gcccgagcgt ggcggccatt
     1741 gtcggcccgt acttcggcgc atcggccgag cacctggttg tggtcggcaa gaccggcttc
     1801 caggagagca aggacggcgg ccagtcctgg caggtggtcg ccccgctgcc agacaagttc
     1861 accgtcggcg gcgtcggccc caactacgcc tgggatccgc acgccgacat cttctacgcg
     1921 tcatccatgg gcaaagacac gctgcggtac atccgacaac cgtaatctct gcgccttcac
     1981 tcgccggact tggctttttt cgccggagcg gtctcgctct tggccgcgcc gttggacttg
     2041 gcttcgctct tcttgctatc actggaagaa gagctgtcgc cgggcgaagc gctctcgctc
     2101 gccggcttat cggcggtggc ggccttcttg tacgaatcgc tgcgatagtc ggtctggtag
     2161 aaaccggagc ccttgaagat cagcccggcc cccgcgccga acagccggcg aagcttcttc
     2221 tttttgcact tggggcactt cttctgcggc tcggccgaga tcgcctcaaa gacttcgaag
     2281 gtgtggtcgc aagcgtcgca gctgtagtcg taagtcggca taccagttca aggttcaagg
     2341 ttcagagttc aaagttccga gttcaaagtt caaggttaat tcggcctggt tcaactttga
     2401 acattgaact tgaaccttga acgtgacaac tcaattatcg tttcgcgccg gcgctcccgt
     2461 tgatacgaag acttgagcag gccggatgac gcggtcgtgc agcttgtagc ccgactgggc
     2521 ggcgcgggtc acagtgccgg cgggatactg gtcgctgggc tcctgggcga tggcctggtg
     2581 ctggttggga tcgaaggccg cgccgaccgt ttcgatccgc acgcattgat gctgcttgag
     2641 gatcgcttcc agctgcgtgg cgaccatctt cacgccgtcg agcagaccct gattggccga
     2701 accctcgctc gcgcttcggg cttgtgtggc ctcgatggcc cgctcgagat tatcgaccac
     2761 ggcaagcatg tcgcggacca gcggcacgac ggcgtacttt cgctcgtcgg cgatgtcgcg
     2821 ctgagcccgc ttgcgatagt tttccagctc agccgtcgcc cgcagggctt tttcattggc
     2881 ttcgcgcagc tcggattcca gccgggcgat cttctcatcg gcgttggtct ggccatttga
     2941 tccgcggttg acgggcatcg tctcggcgtc agcttc
//
LOCUS       DASRJB010000178         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2306599, whole genome shotgun sequence.
ACCESSION   DASRJB010000178 DASRJB010000000
VERSION     DASRJB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306599"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2045
                     /locus_tag="VFB80_04930"
     CDS             <1..2045
                     /locus_tag="VFB80_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33136.1"
                     /translation="AYLQALLNKRDNNPLTKPTAAKKVQLEAFLFDGNTTSQLRKLNL
                     SKTAGGAFDPAYLAEQLTTKGQVTALGSVVHEVGQQFGSGRLAGVVLFSDFAHNSGLA
                     PLGAGPQSPATKLGVPVYAVGLGATEAIDLAVDWQTDPKMKKAERSSLLVKLRQSGLD
                     GQAASVRVVGHKISGEAGSAAGAEIQIGQKNVTLGNAVETVELPFTPEDSGRFEFTAT
                     VEKLDGEVVDQNNQAVRQVNIIDDYLRLMYVAYEPTWEWRFIKEVFHRDKLVGMQGFR
                     TFLSSSDPRVRESNVLFLPTLTPKRSEFFANDVLFLDDVPRSGLSDRFCEMAKEYVSN
                     LGGGLVVIAGPRFGPRELYQTPLADMLPVIIDPAGELRDEPEHPEFRVRLTPHAARYP
                     FMQMGANEVENSRAWDTLGKLPWYQPVAALHEQAFALAEHPTDKCADGKTPQPLIAVR
                     QYGKGEVVYLGFNETWRLRRKYGEKYYRQFWSQLIYRLGMSHALGAEKRFVVRVDQQQ
                     YRAEDKVTLSVEAYDENYEPLGDENLPDRGLVAEMTVPGKSGEQTTTFTVPMLRRGLF
                     EARIPVFAVGAYSLKVKDPITGKTDEQRFDVTPLSAERRRGVRDDKLQNELAQQTGGR
                     AYDLTTVSRLPDDLKLEPVVERLTRNYRLWTTPLWFGVVVVLMLGEWLSRKIIRLS"
     gene            2042..>3295
                     /locus_tag="VFB80_04935"
     CDS             2042..>3295
                     /locus_tag="VFB80_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33137.1"
                     /translation="MTTALKKPAAPSVTKLATLRAQLATLRRARAGVRGAVAWSALAI
                     AILAALAAVLALDLIFELAVPQRIVVLLLAAGAVAWAFWRYTRPHLGVTEDETEMALL
                     VERQQQIDSDLVAALQFESPQSYDWGSPQLTGAVIDYVAAVGSGINVFEGFSRQQMGR
                     RLGLLGVIAAAALIAAAIWPGHLAAFMNRLLLGSRHYPTRTVIEQVVVNRTPVLVRQS
                     GGNAPADSNGAQGRPVTFLVQCSGELPAAGTAQLAAIGASRSRAKLELKPLTLAERLA
                     RLEEARRKLNEALAAAAGELDLPWKAEVSALLRFDAPQADSVLVKAKTHANLPDVLTA
                     LAQSTTAWPGDRAKTAVLSGELARLNDGLTYKLYAGDAWTDVARITMIPLPIVELLVK
                     PLPPKYAQSALQVAPDPAGRQLAVLE"
BASE COUNT          567 a         1089 c         1112 g          527 t
ORIGIN      
        1 tcgcctacct tcaggccctt ctgaacaagc gcgataacaa cccgctcacg aaacccaccg
       61 ccgcgaagaa ggtgcagctc gaagccttcc tgttcgacgg caacacgacg agccagctcc
      121 gaaagctgaa cctgtcgaaa accgccggcg gcgctttcga tccggcatat cttgccgagc
      181 agctgacgac gaaggggcaa gtcacagcgc tcggctcggt ggttcacgaa gtcggccagc
      241 aattcggctc cggccggctg gcgggcgtcg tgctgttcag cgatttcgcc cacaactcgg
      301 gccttgcgcc gctcggtgcg ggtccgcagt cgccggccac gaaactgggc gtgcccgttt
      361 atgccgtggg cctcggggcg acggaagcga tcgacctggc cgtcgattgg cagaccgacc
      421 ccaagatgaa aaaggccgag cggagcagcc tcctggtgaa gctgcggcaa agcggcctgg
      481 acggccaggc cgcgagcgtc cgcgtcgtcg gccacaagat cagcggcgag gccggcagcg
      541 cggccggggc cgaaattcag attggccaga agaacgtgac gctgggcaat gcggtcgaaa
      601 ctgtcgagct gcccttcacg cctgaagact ccggccgctt tgagttcacg gcgacggtcg
      661 agaagctcga cggcgaagtc gtcgatcaga acaaccaggc cgtgcggcag gtcaacatta
      721 tcgacgacta cctgcggctg atgtacgtcg cctacgagcc gacctgggag tggcggttca
      781 tcaaagaggt ctttcaccgc gacaagctgg tcgggatgca gggttttcgc acgttcctca
      841 gctcttccga cccgcgggtc cgcgagtcga acgtcctgtt cctgccgacg ctgacgccca
      901 agcgcagcga gttctttgcc aacgatgtgc tgttcctcga cgacgtgccg cggagcggcc
      961 tctcggaccg gttctgtgag atggcgaagg aatacgtcag caatctcggc ggcggcctgg
     1021 tcgtgatcgc cggcccgcgg ttcggcccgc gcgagctgta ccagacgccg ctggccgaca
     1081 tgctgccggt gatcatcgac ccggccggcg aattgcgcga cgagccggag cacccggagt
     1141 tccgcgtccg cctgacgccg cacgccgccc gctatccgtt catgcagatg ggggccaacg
     1201 aagtcgaaaa cagtcgagcc tgggacaccc tggggaagct cccctggtat cagccggtgg
     1261 cggcgctgca cgagcaggcg ttcgccctgg ccgagcaccc cacggataag tgcgccgacg
     1321 gcaagacgcc gcagcccttg attgccgtgc ggcagtacgg caagggcgaa gtggtgtatt
     1381 tgggcttcaa cgagacctgg cgattgcggc gcaagtacgg cgagaagtat taccggcagt
     1441 tctggtcgca gctcatctac cgcctgggta tgagccacgc cctcggggcc gagaagcggt
     1501 tcgtcgtccg cgtcgatcaa cagcagtacc gggcggaaga caaggtcacg ctctcggtcg
     1561 aggcctacga cgagaactac gagccgctgg gtgacgagaa cctgcccgat cgcgggctgg
     1621 tcgccgagat gacggtgccg ggcaagagcg gcgagcagac gacgacgttt acggtgccga
     1681 tgctccgccg cggcctgttc gaggcccgca ttccggtgtt tgccgtcggg gcgtacagcc
     1741 tgaaggtcaa ggacccgatc accggcaaga ccgacgagca gcgattcgac gtgacgccgc
     1801 tctcggccga gcgccgccgc ggcgttcgcg acgacaagct gcagaacgag ctggcccagc
     1861 aaaccggcgg gcgggcctac gacctgacca ccgttagccg cctgcccgac gatttgaagc
     1921 tggaacctgt ggtggagcgg ctgacccgca actaccggct gtggacgacg ccgctctggt
     1981 ttggcgtggt cgtcgtgctg atgctcggcg aatggctctc ccggaagatt atccgactgt
     2041 cgtgacaacc gccctgaaaa aacccgctgc cccctcggtg acgaagctcg ccacgttgcg
     2101 tgcccagctg gcgacgttgc gccgcgcccg ggccggcgtc cgcggagccg tcgcctggtc
     2161 ggcactcgcc atcgcgatcc ttgccgcgct ggcggcagtg ctggcgctgg acttgatctt
     2221 tgagctcgcc gtgccgcagc gaatcgtggt gctgctcctc gcggccggcg ccgtggcctg
     2281 ggcgttttgg cgctacacgc ggccgcactt gggcgtgacg gaggacgaaa ccgaaatggc
     2341 gctgctggtc gagcggcagc agcagatcga cagcgacctg gtggcggcgc tgcagtttga
     2401 atcgccgcag tcctatgact ggggctcgcc gcagctcacc ggagccgtta tcgactatgt
     2461 cgccgccgtc ggaagcggga ttaacgtctt cgagggcttt tcgcgccagc aaatgggccg
     2521 ccgcctcggc ctgctcggcg tcatcgcggc ggcggccctg atcgcggctg ccatctggcc
     2581 ggggcacctc gccgcgttca tgaaccgcct gctgctcggc tcgcggcatt atccgacgcg
     2641 cacggtcatc gagcaggtcg tcgtcaatcg cacgcccgtg ctggttcgcc aaagcggcgg
     2701 caatgccccg gccgacagca acggcgcgca gggccggccg gtgacattcc tggtgcagtg
     2761 ctccggcgaa ctgccggccg ccggcacggc ccagctggcc gcgatcggcg cgagccgcag
     2821 ccgcgcgaag ctggagctca aaccgctgac gctggccgag cggctggccc gattggaaga
     2881 agcacggcgg aagctgaacg aagcgctcgc ggccgcggcg ggcgaactgg acttgccttg
     2941 gaaggccgaa gtctcggcgc tattgcgttt cgacgcgccg caggctgact ctgtcctcgt
     3001 gaaagcgaag acgcatgcca atttgccgga cgttctgaca gcactggccc agtccaccac
     3061 tgcctggccc ggcgacagag ccaaaacggc ggtcctctcg ggcgagttgg cccggctcaa
     3121 cgacggcctg acctacaagc tgtatgccgg cgacgcctgg accgatgtgg cccggattac
     3181 gatgatcccg ctgccgatcg tcgaattgct ggtcaagccg ctgccgccca agtacgcgca
     3241 aagtgcgctg caggtcgctc ccgatcccgc gggccggcaa ctcgcggtgc tggag
//
LOCUS       DASRJB010000179         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4000805, whole genome shotgun sequence.
ACCESSION   DASRJB010000179 DASRJB010000000
VERSION     DASRJB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000805"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFB80_04940"
     CDS             complement(<1..1126)
                     /locus_tag="VFB80_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HZN33138.1"
                     /translation="MQDFERLGVFYLGKQYDLAAGKPQDELVLYDSKDLTTHAVCVGM
                     TGSGKTGLCIALLEEAAIDGIPAIVIDPKGDLANLLLTFPELRPADFQPWIDPADAKR
                     TGVSPEQFAQQTAEKWKKGLAEWGQDGARIQRLKDAADVAIYTPGSSAGLPLTVLRSF
                     DVPPVSTIENNEALRDAISAAASGLLGLMGIDADPLRGREHILLSSIFDKAWRSGTSL
                     DMAALIRSIQTPPLDKVGVMDLESFFPAKERFELAMQLNNLLASPGFSAWMEGEPLDI
                     QRLLYTPEGKPRLAILSIAHLSDAERMFFVTILLSETIGWMRSQSGTSSLRALLYMDE
                     VFGYFPPSANPPSKTPMLTLLKQARAFGLGVVLATQNPVDL"
     gene            1212..2708
                     /locus_tag="VFB80_04945"
     CDS             1212..2708
                     /locus_tag="VFB80_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33139.1"
                     /translation="MITLATCVLTLLAAETDRPFAIEVVDEATGRGVPLVELTTTSGV
                     TFITDSAGLVAFDEPGLMNQLVYFAVKSHGYEVKKDGFGFPGVRLEIKSGGSGTIKIK
                     RLNIAERLYRVTGQGIYRDSVQLGRQPPIKEPLLNAKVAGQDSVQNAIYQGKLRWFWG
                     DTTWPAYPLGNFHMPGAVSRLPADGGLDPAVGVDLTYFTGENGFARGTAEMPGNGPTW
                     IGGVAVLPDKEGRERLLCGYAKIKAPMEVYRRGIAQWNDETERFDRVSDFEPTVPPYP
                     DGHPLRYRDGNTDYIYFASPYPLVRVRANAESYLDLSAYEAYTCLKPGAKTSDGEIDR
                     DDAGLVRYAWKRNTPPVGPGEQAKLVNSGKLKAEETLLQLRDAATGKAVTTSSGSVNW
                     NAYRKRYVMITSELFGSSMVGEIWYAEADSPIGPWSKAVKIVTHDKYSFYNPKQHPYF
                     DQDGGRVIYFEGTYTHTFSGNEHRTPRYDYNQIMYRLDLADERLQPAH"
     gene            complement(2744..>3295)
                     /locus_tag="VFB80_04950"
     CDS             complement(2744..>3295)
                     /locus_tag="VFB80_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33140.1"
                     /translation="EKYGVDDPKFERDGAPRMVRNFCIARRLVEAGARYVSMNFIRWD
                     WHGGDGMNFVNSKRDFPLLDSGLSSLLTDLHERGLDKDVSVVVWGEFGRTPRLNNRNS
                     RDHWPQANAAIIAGGGMRLGQVIGATNKYAEHPVERPVKFEEIFATLYAKAGLSLNTR
                     EFDLRGRPQYLVEPGIEPMRELM"
BASE COUNT          592 a         1065 c         1080 g          558 t
ORIGIN      
        1 cgagatcgac aggattctgc gtcgccagca ccacgcccag accgaacgcg cgggcctgct
       61 tcagcaaggt cagcatcggc gtcttgctgg gcgggttggc cgacggcggg aagtagccaa
      121 acacctcgtc catgtagagc agcgcccgca ggctcgacgt gcccgattgg ctccgcatcc
      181 agccgattgt ttcgctcagc aggatcgtca cgaagaacat ccgctcggcg tccgagaggt
      241 gggcgatcga caggatcgcc agccgcggct tgccttcggg cgtgtacagc agccgctgga
      301 tgtcgagcgg ctcgccttcc atccaggccg agaagccggg cgacgcgagc agattgttga
      361 gctgcatcgc cagctcgaag cgctccttcg ccgggaagaa gctctccaaa tccatcacgc
      421 cgaccttgtc cagcggcggc gtctggatcg agcggatcag cgccgccatg tcgaggctcg
      481 tcccggatcg ccaggctttg tcgaaaatgc tcgacagcaa aatgtgctcg cggccgcgga
      541 gcggatcggc gtcgattccc atgaggccca agaggcccga cgccgcggcg ctgatcgcat
      601 cgcggagggc ctcgttgttc tcgatggtgc tcaccggcgg cacgtcgaac gaccgcagca
      661 ccgtcagcgg caggccggcg ctgctgccgg gcgtgtagat ggccacgtcg gccgcgtcct
      721 tcagccgctg gatccgcgca ccgtcctggc cccactcggc caaacccttt ttccatttct
      781 cggccgtttg ctgggcgaat tgctcggggc tgacgccggt ccgtttggcg tcggcgggat
      841 cgatccaggg ctgaaaatcg gccgggcgca attccggaaa cgtgagcagc agattggcca
      901 ggtccccctt ggggtcaatg acgatcgcgg ggatgccgtc gatcgccgcc tcttcgagca
      961 gcgcgatgca gaggcccgtc ttgccgctgc cggtcatgcc gacgcagacc gcgtgcgtcg
     1021 tcaagtcctt ggagtcgtac agcaccagct cgtcctgcgg cttgccggcc gccaggtcgt
     1081 actgcttgcc gaggtagaaa acgccgagcc gttcgaagtc ttgcatggct gctcgcccta
     1141 ggcgaagtga gagaaggctc gtattctacc agcggcgttt tcgccagtgg gctcctcaaa
     1201 ccgtgtctgc catgatcacg cttgccacct gcgttctgac cttgctggcc gcggagaccg
     1261 accggccctt cgccatcgaa gtcgtcgatg aagccaccgg ccgcggcgtg ccgctggtcg
     1321 agttgacgac gaccagcggc gtcacgttca tcaccgactc ggccggcctg gttgcgttcg
     1381 acgagccggg gctgatgaat cagctcgtct atttcgcggt caagagccac ggctatgaag
     1441 tcaagaagga cggattcggc tttcccggcg tgcggctgga gatcaagtcc ggcggctccg
     1501 gcacgatcaa gatcaagcgg ctgaacatcg ccgagcggct gtatcgcgtg acggggcagg
     1561 ggatctatcg cgacagcgtg cagttgggga ggcagccgcc gataaaggag ccgctgctca
     1621 acgccaaggt cgccggacag gacagcgtgc agaatgcgat ttaccagggg aagctgcggt
     1681 ggttttgggg cgacacgacc tggccggcct atccgctggg caactttcac atgcccggcg
     1741 ccgtcagccg cttgccggcc gacggcggcc tcgacccggc cgtgggggtc gatctgactt
     1801 atttcacggg cgagaacggc tttgcccgcg gtaccgccga gatgcccggc aatgggccga
     1861 cgtggatcgg cggcgtggcg gtgcttccgg acaaggaagg ccgcgagcgg ctgctctgcg
     1921 gctatgccaa gatcaaggcc ccgatggaag tctatcgccg cgggatcgcc cagtggaacg
     1981 acgagaccga gcggttcgac cgcgtcagcg atttcgagcc gacagtcccg ccctatcccg
     2041 atgggcatcc gctgcgctat cgcgacggga atacggacta catctatttc gcgtcgccct
     2101 atccgctggt ccgcgtgcgg gccaatgccg agagctattt ggacctcagc gcatacgaag
     2161 cctacacctg cctcaagcct ggggcgaaga cttcggacgg cgagatcgac cgcgacgacg
     2221 ccggactggt tcggtacgcc tggaaaagga acacgccgcc cgtcggtcca ggcgaacagg
     2281 ccaagctggt caatagcggg aagttaaagg ccgaggaaac cctgctgcag ctgcgcgatg
     2341 ccgcgacggg caaggcggtg acaacgtcct cgggctccgt gaactggaac gcgtatcgca
     2401 agcggtatgt catgatcacc tccgagcttt tcggcagctc catggtcggc gaaatctggt
     2461 acgccgaggc cgatagcccc atcggcccgt ggtccaaggc ggtcaagatc gtcacgcacg
     2521 acaagtacag cttctacaac cccaagcagc acccgtactt cgaccaggac ggcgggcgcg
     2581 tgatctattt cgagggcacc tacacgcaca ccttcagcgg caacgaacac cgcacgccgc
     2641 ggtacgacta caaccagatc atgtaccgcc tcgacctggc cgacgagcgg ctgcaaccgg
     2701 cccattagtc cgcaggccgc agaaacgcca cagagggcgc tggctacatc agctcccgca
     2761 tcggctcgat gccgggctcg accaggtact gtgggcggcc acggaggtcg aactcgcggg
     2821 tgttcagcga gaggccggcc ttggcgtaga gcgtggcaaa aatctcctcg aacttcaccg
     2881 gccgctcgac gggatgctcg gcgtacttgt tggtcgcgcc gatcacctgg cccaaacgca
     2941 tgccgccgcc ggcgataatt gcggcgttgg cctgcggcca gtggtcgcgg ctgttgcggt
     3001 tgttcagccg cggggtgcgg ccgaactcgc cccagacgac gacggaaacg tccttatcga
     3061 ggccgcgctc gtggaggtcg gtcagcagcg acgacaggcc gctgtcgagc agcgggaagt
     3121 ctcgcttgga gttgacgaag ttcatgccgt cgccgccgtg ccagtcccag cggatgaaat
     3181 tcatcgagac gtagcgtgcg cccgcttcaa ccaggcggcg ggcgatgcag aagttgcgca
     3241 ccatccgcgg cgcgccgtcg cgctcgaact tgggatcatc gacgccatac ttctc
//
LOCUS       DASRJB010000180        11146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2307165, whole genome shotgun sequence.
ACCESSION   DASRJB010000180 DASRJB010000000
VERSION     DASRJB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307165"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFB80_04955"
     CDS             complement(<1..680)
                     /locus_tag="VFB80_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN33141.1"
                     /translation="MIAHQHRVLIVEDQESERRALERVLRVADYDVATASSAEEALDW
                     RDQPIDLVISDLRMGGKSGIDLLLDWRAVKPQTPFLLATAYGDVDSAVRCMKLGAYDY
                     LTKPVDPERLLNIVKSAITEAASREEAPAAAQVEQYDLPKQATFGPLIGNSRPMQEVF
                     ERVRRVSQTDSIVLILGESGTGKELVAAAIHRESPRAAGPYVAVNMAAIPAHLVESEL
                     FGHVRGAFT"
     gene            complement(677..2074)
                     /locus_tag="VFB80_04960"
     CDS             complement(677..2074)
                     /locus_tag="VFB80_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN33142.1"
                     /translation="MGEPIPPKSRGSTISIWSCVIAAVAVVGLLGGGIAAFDVQRAVD
                     DLRGEELRRLQFNAERTGAQIEGQLLESKSRDVASIKEAPWLRRYWSQNLMKQSGRLY
                     AAVVDRAGRIVAHTNPDLQGRQPASADFDATESSPATRLFTGSDEVLTLGEGAINVQV
                     SIYGGGDFLGSYLTGLDAGWLDERLAAERWSRARFWAILVAGMCALLLLSSVAVVRVT
                     RHTARLEYELDAAHARRISEMHELVLGIAHEIRNPLNAIRLNLHTIGQVFRDEAALDD
                     AEIRSMLGEMTREITRLENLMREMLGFVQTSADPPAPVDVSDEVQRAVSFFGPNLERR
                     GVQVRLDVGEACRVAMDGRRLRQVLLNLLNNALEALPAGGNIEISVQRRRGQIEVSVT
                     DDGPGIRTEDRERVFVPFYSTKASGTGLGLALARKFVEEAGGTIACEERPDRPGCRFR
                     IVLPASTGAEVELAT"
     gene            complement(2257..3126)
                     /locus_tag="VFB80_04965"
     CDS             complement(2257..3126)
                     /locus_tag="VFB80_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33143.1"
                     /translation="MGVAKYLKFAFLNRWNLLAFSGGMALAFISGHADIVAPLVLAAE
                     AAYLGLLGTHPKFQKHVDAQSAAVERKEKSETSQQTLNQILRALPGTALSRFEKLRAQ
                     CLDLRQIAADLANSRDSAGAPLDSLQVASFDKLLWMFLRLLFTEHSLTKFLERTNLRS
                     IQNEIDALRQRLGKISANDESAHAQKMRRTLEDNLQTSQDRLANYQKAKSNHELVQLE
                     LDRLENKIKSLAEISVNRQEPDFISAQVDQVAGSMLETEKTMNELQFVTGLSAQDEEV
                     PDLVQNPQVMIMR"
     gene            complement(3217..4113)
                     /locus_tag="VFB80_04970"
     CDS             complement(3217..4113)
                     /locus_tag="VFB80_04970"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN33144.1"
                     /translation="MTASNPTTTAAPAQPIGMGEVLDHVRQPEEIVRPAVVSFRNVTK
                     TYNAGTPAAFTAIKDVSFVVEDLPDKGEFIAILGPSGCGKSTILRLIAGLEPQHPATS
                     GEVLVLGQPVAGPGADRGMVFQDYTSFDHRTVLENVTFGLECQGVPRKQRFERGREWI
                     AKMGLNVAKDQNKFPHELSGGMRQRVAIARTLILHPRIILMDEPFGALDPNTRCNMQD
                     LLVKLWKEVQATVFFITHSIEEAVFLGDRVYLMSNSPGTILEEMQIEPPDRPAHQMLV
                     LPRFLDTVHYIRDKITQLEVSN"
     gene            complement(4117..5283)
                     /locus_tag="VFB80_04975"
     CDS             complement(4117..5283)
                     /locus_tag="VFB80_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN33145.1"
                     /translation="MSVPATSAPPPQPARTGSRGWLGDWFTLRKDGPLWESVLLGTAC
                     VVLCFALWWLLTLGEPAEKRFIGPMTLPSPRETLDPRNDLWPPPGADLTKWKPNRERF
                     WSERKDLAINTLVTLRRVGLGFLLAVVVGVPLGIVAGCYPRFNSFLMPVVIFGRNIPL
                     AAVLPLLLYMFQHGEQRKIMFIFVACVAFVIADTAQAIRDVAGRYIDTAYTLGAGRWQ
                     TIMKVLVPLAMPAVFSSFRVLFGLAFGYIMLAESIKEPDDVGGLGFQINTFQRRGPRE
                     FIYLIILIIPLVALAVDQVLFWIQRQLFPHVYGGAGWLNKLVRLVLHLWDDVKRVFFQ
                     IGPEVPEQTPASAPSQNKTVNDSAAQDGGKEGKREGEKSEQVAPSLPPSVPPSQ"
     gene            complement(5481..7262)
                     /locus_tag="VFB80_04980"
     CDS             complement(5481..7262)
                     /locus_tag="VFB80_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HZN33146.1"
                     /translation="MAGQPKTPFYVAVLLVVTGLIAFSAWRMLRNQPGPVPPMHQAGN
                     QEQKTNGGKPDGGTPNGGTPATGAAEAADTAGITTVKEFTHKPSERLPAVKGTAAYKP
                     MENNTVRFALNVWAGWAPIIYANNGFKADKAWKTADGKDFRVELVLMDDPIQMRDSYT
                     AGNVHIGWATLDMLPLFVDGFVDSSGNPRDSRIMPRVYQQVDWSNGGDGIVCRDTIKT
                     VADLRGKTIALAENSPSHYFLLNMLVAGGLQPAEVKMFGTPTAFEAAAAFNTRKDIAA
                     AVSWAPDIYKLSEVKGNRMLVTTGTANKLIADVWYARADFAQDHPDICEGLVRGIFDA
                     MADLKQDANKQKCAELMATGYGLQPADALGMLGDAHSTNWGENYQFFVNQNNPTNFAR
                     VWNQADYLYRSVRLLSNQQVPFDQVMDYSIIAKLGTEPKYAAQKDEYGVQFAPKPTAA
                     VRGEEEILTNTVVIHFYPNSWEIFKKVTKDQEGKTIEVDYDPKAKYVIDEIGKLAGQF
                     GNARIIIEGHTDSSMKDKIPAEMVKELSLNRAKAVKEEVIKKFNFDFNKFNVEGFGWD
                     RPADPSDPLNQAQNRRVEVKIFTAEAN"
     gene            complement(7389..7685)
                     /locus_tag="VFB80_04985"
     CDS             complement(7389..7685)
                     /locus_tag="VFB80_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33147.1"
                     /translation="MATGKQSWLDEKAQTPVIDAYVQKLSTFVQAMADGKIDDHELAA
                     QEQRLSAVMKEVEPTLSDAQHQQVTKLLCELTAYNIMQTLNSLEQARPHAKFRG"
     gene            complement(7855..8238)
                     /locus_tag="VFB80_04990"
     CDS             complement(7855..8238)
                     /locus_tag="VFB80_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33148.1"
                     /translation="MSARILVVDDDHDNADSLARLVQILGQEPIVAYSGAQAIRAAEE
                     QQPELALIDIGMPGMDGYETAARIRRSTGGRTILVAVTGWSRAEDKQRATDAGFDLHY
                     SKPISVDALQELLEIPDLASAAAAT"
     gene            complement(8795..10423)
                     /locus_tag="VFB80_04995"
     CDS             complement(8795..10423)
                     /locus_tag="VFB80_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN33149.1"
                     /translation="MSKPSQRYDCLIIGAGHNGLVAAAYLARGGKKVCVLERRHVLGG
                     CAVTEELWPGFKISVASYVISLFRPEIIRELRLKQYGLSILPRNPSSFTPLLDGRSLL
                     MGPDERATCGEIAKFSRQDAERYPAYNRLLERVAAVLEPILAKAAPDPLPLPAEWRKI
                     GVGKRLRDAGRLLELYQSIGRLGEDLPEAIELLIGPARPILERWFEAEVLRTTLATDA
                     VIGAFASPSYPGTAYVLLHHVMGEAGGSRGVWGYVQGGMGGLTAALEKACHDLGVEIR
                     RESSVKRILVSKGQVAGVTLWDDSLLEAPIVASSIDAHQTFERLLDPAELPAEFRTAV
                     AKIDYASASAKINVALSELPNFTCLPSTVIAPHHRGTIHISPTLDYMERAFDDAKYGS
                     PSSEPILEITLPTSVDHTLAPPGQHVMNIFVQYAPYKLADGQSWDDIKEEFGDRCLDL
                     LARYAPNIKNAVLHRQVLTPLDLERLIGLTGGNIMQGAMTPQQLFMMRPVPGWADHRT
                     PIRGLYLCGAATHPGGGVLGACGRNAAEEILRDG"
     gene            10455..10889
                     /locus_tag="VFB80_05000"
     CDS             10455..10889
                     /locus_tag="VFB80_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33150.1"
                     /translation="MLLLLVVSTPAESGLSVMAVILVPASGDDLTINWWNWRPTVALL
                     VRAGVLPPGERADRCESNGCGGYLSATEAIQAADHIESLVARMATNERLLSRGEIVAV
                     PESDKPVSEWTEEEAYQRYAARRELLVPFADFCRRSGGFQVV"
     gene            10913..>11146
                     /locus_tag="VFB80_05005"
     CDS             10913..>11146
                     /locus_tag="VFB80_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN33151.1"
                     /translation="MKSPVVYLAAAWSLAFFGSAFAADRPPNFVVIFADDLGYGDLGC
                     YGHPTIKTPHLDRMAREGLRFTQFYSAAEVCTPS"
BASE COUNT         1890 a         3607 c         3560 g         2089 t
ORIGIN      
        1 gtgaacgcgc cgcggacgtg gccgaacaac tcgctttcca ccaggtgggc cgggatcgcg
       61 gccatgttca cagccacgta cgggcccgcg gctcgcggac tctcgcggtg aatcgccgcc
      121 gctaccagct ccttgcccgt gcccgattcg cccagaatca gcacgatgct gtcggtctgc
      181 gaaacgcggc ggacccgctc gaagacctcc tgcatgggcc gcgaattgcc gatcagcggc
      241 ccgaaagtcg cttgcttcgg caggtcgtac tgctccacct gcgcagcggc aggcgcctct
      301 tcgcggctgg cggcctcggt gatcgccgat ttcacgatgt tcagcagccg ctcgggatcg
      361 acgggcttgg tcagataatc gtaggcgccg agcttcatgc agcggacggc cgaatcgacg
      421 tcgccgtagg ccgtggccag caggaaggga gtctgcggct tcacggcccg ccagtcgagc
      481 aggaggtcga tgccgctctt gccgcccatc cgcaggtcgc tgatgaccag gtcgatcggc
      541 tggtcccgcc agtcgagcgc ttcctcggcg ctgcttgcgg tggccacgtc gtagtcggcg
      601 acgcgcagca cccgttccag ggccctccgc tccgactcct ggtcctcgac aatgagcacg
      661 cgatgctggt gggcgatcat gtagccaact ccacttcagc gccggtgcta gccggcagga
      721 caatgcgaaa ccggcagccg ggccggtcgg gacgctcctc gcaggcgatg gtgccccctg
      781 cttcctcgac gaacttgcgg gccagcgcca ggcccaggcc ggtgccactg gctttggtcg
      841 aatagaacgg cacgaacacc cgctcgcgat cctccgtgcg gattcccggg ccgtcgtcgg
      901 tgacggagac ttcgatttgc cctcggcggc gctggacgga aatctcgatg tttccacccg
      961 cgggcagcgc ttccagcgcg ttgttgagca ggttcagcag cacctggcgg agccggcggc
     1021 cgtccatcgc cacccggcag gcttcgccga cgtccagccg cacctgcacg ccgcgccgct
     1081 ccaggtttgg tccgaaaaag ctgacggccc gctgcacttc gtcgctcaca tcgacggggg
     1141 cgggcggatc ggcgctggtc tggacgaagc ccagcatttc gcgcatgagg ttttccagcc
     1201 gcgtaatctc gcgggtcatt tccccgagca tcgagcggat ttcggcgtcg tccagggccg
     1261 cctcgtcgcg gaacacctgg ccgatcgtgt gcaggttgag gcggatggcg ttgagcggat
     1321 tgcggatttc gtgggcgatg cccagcacca gctcgtgcat ctccgagatt cgccgggcgt
     1381 gggctgcgtc gagctcgtat tccagccggg ccgtgtgccg cgtcacgcgc acgaccgcca
     1441 cgctggacaa gaggagcagc gcgcacatgc cggccacgag aatggcccag aaccgcgccc
     1501 tgctccagcg ttcggcggcg aggcgttcgt cgagccagcc cgcgtcgagc ccggtaaggt
     1561 agctgcccag gaaatcgcct ccgccgtaga tcgacacctg cacgttgatg gccccttcgc
     1621 ctaaggtcag cacctcgtcg gagccggtga agagccgggt cgccggcgag gactcggtgg
     1681 catcgaagtc cgctgacgcc ggctgccgcc cctgcagatc ggggttggtg tgggcgacga
     1741 tccggcccgc tcggtcaacc actgccgcgt agaggcggcc ggactgcttc atcaggtttt
     1801 gcgaccagta gcggcggagc cacggcgctt ccttgatgga agcgacatcc cgcgacttgc
     1861 tttccagcaa ttgcccttcg atttgcgcgc cggtgcgttc ggcgttgaat tgaaggcggc
     1921 gcaattcctc gccgcgcagg tcgtccacgg cccgctgcac gtcgaatgcc gcgatcccgc
     1981 cgccgagcag tccgaccacc gcgacggcgg cgatgacgca gctccagatc gaaatcgtgc
     2041 ttccccgcga ttttggcgga atgggctcgc ccatgatgcc gacttgcaaa tgcaggcgtg
     2101 cgaagcccgc tccccaacaa accaagcgaa taggtcccca ctggcattct gccgcgtacc
     2161 ggcgcaaatc tcaagcgaca aattcctggg ccgcggcaat atgtcgcggc cccctgcgcg
     2221 ggacagtaca gtgcggcacg ccgcggcgca cgagcgctac cgcatgatca tcacttgcgg
     2281 gttctgcacc aggtcgggca cttcctcgtc ctgcgccgag aggcccgtga cgaactgcag
     2341 ctcgttcatc gtcttctcgg tctccagcat gctgccggcg acctgatcga cctgcgccga
     2401 gatgaaatcc ggctcctggc gattgaccga gatttcggcc agcgacttga tcttgttttc
     2461 caggcggtcg agctccagct gcacgagctc gtggttggac ttggcctttt gatagttggc
     2521 caggcggtcc tgcgacgtct gcaaattgtc ttcgagcgtg cggcgcatct tttgcgcatg
     2581 cgccgactcg tcgttggccg agatcttgcc cagccgctgc cgcagcgcat caatctcgtt
     2641 ctggatcgaa cgcaggttcg tccgctccag gaatttcgtc agcgagtgct cggtgaagag
     2701 cagccgcaag aacatccaca gcagcttgtc aaagctggcg acctgtagcg aatcgagcgg
     2761 cgcaccggcg ctatcccgcg aattggccag gtcggcggcg atctgccgca ggtcgaggca
     2821 ctgggcccgc agcttctcga accgcgacag ggccgtgccc ggcagcgcac ggaggatttg
     2881 attgagcgtc tgctgcgacg tttccgactt ctctttgcgc tcgaccgcgg ccgactgagc
     2941 atcgacgtgc ttctgaaact tggggtgcgt gcccaagagg cccagatagg ccgcctccgc
     3001 cgccagcacc agtggcgcga cgatatccgc gtggccggaa atgaatgcca gcgccatccc
     3061 accggaaaac gccagcaggt tccagcgatt gaggaaagcg aacttgaggt atttggctac
     3121 gcccatcgcc gcgactcaca ctaacccgaa gcgtcagcga gggacttcag gggcaggccg
     3181 ggtccaaaag tccctcgctc acgcttcggg ttggcattag ttggaaacct ccagctgcgt
     3241 gatcttatct cgaatatagt ggaccgtatc caagaagcga ggcagcacca gcatctggtg
     3301 cgccggccgg tcgggaggct cgatctgcat ctcttccagg atcgtcccgg gcgaattgct
     3361 catcagatag acgcggtcgc cgaggaacac cgcttcctcg atcgagtgcg tgatgaagaa
     3421 caccgtcgcc tggacttcct tccaaagctt caccagcagg tcctgcatgt tgcagcgagt
     3481 gttcgggtcg agggccccga acggctcgtc catcaggatg attcgcgggt gcaggatcag
     3541 cgtgcgggcg atcgccaccc gctgccgcat gccgcccgat agctcgtgcg ggaatttgtt
     3601 ctggtccttg gccacgttca ggcccatctt ggcgatccac tcgcggcctc gctcgaaacg
     3661 ctgcttgcgc ggcacaccct gacactccag gccgaacgtg acgttctcca gcaccgtgcg
     3721 gtggtcgaag ctcgtgtaat cctggaaaac catcccgcgg tccgcgcccg gaccggcgac
     3781 cggctggccc aggacgagca cctcgccgct cgtcgccggg tgctgcggct ccaggccggc
     3841 aatcagccgc aggatcgtgc tcttgccgca gccgctgggg ccgaggatgg cgatgaactc
     3901 gcccttgtcg ggcaagtcct cgacgacgaa cgagacatcc ttgatcgccg tgaaggcagc
     3961 cggcgtgccg gcgttgtagg tcttggtcac attgcgaaac gaaaccaccg ccggccggac
     4021 gatctcctcc ggctgccgga cgtggtcgag cacttcgccc atgccgatgg gctgagcggg
     4081 cgcggccgtc gttgttggat tggatgcagt cacttgctat tgcgacggag ggacggaggg
     4141 agggagcgaa ggagcgacct gctctgactt ctctccttct ctctttccct ccttccctcc
     4201 gtcctgggct gcggaatcgt tcacggtttt gttttgcgat ggagcagaag cgggcgtctg
     4261 ctcgggaacc tccggtccga tctgaaagaa gacgcgcttc acgtcgtccc aaaggtgcag
     4321 gacgaggcgg accagcttgt tcagccagcc ggcaccgcca tagacgtgtg gaaagagctg
     4381 ccgctgaatc cagaacagga cctgatcgac ggccagcgcg acgagcggaa tgatcaggat
     4441 aatcaggtag atgaattccc gcgggccgcg cctctgaaac gtgttgatct gaaaccccag
     4501 gccgccgacg tcgtcgggtt ccttgatcga ctcggcgagc atgatgtagc cgaaggccag
     4561 cccgaacaac acccggaacg agctgaacac cgcgggcatc gccagcggga cgaggacttt
     4621 catgatcgtc tgccagcggc cggccccgag cgtgtaggcc gtgtcgatgt agcggccggc
     4681 tacgtcgcgg atggcctggg ccgtgtcggc aatcacgaac gccacacagg ccacgaagat
     4741 gaacatgatc ttccgctgct cgccgtgctg aaacatgtag agcaagagcg gcagcaccgc
     4801 agccagcggg atgttgcggc cgaaaatgac caccggcatc aggaacgaat tgaaccgcgg
     4861 atagcagccc gccacaattc ccagcggcac gccgacgaca accgccaaga gaaaacccag
     4921 gcccacgcgg cggagcgtca ccagcgtgtt gatcgccagg tccttccgct cactccagaa
     4981 gcgctcgcgg ttgggcttcc acttcgtcag atccgcgccg ggcggcggcc agaggtcgtt
     5041 gcgcgggtcg agcgtctccc gcgggctggg cagcgtcatc ggcccgatga accgcttttc
     5101 cgccggctcg cccagcgtca gcagccacca aagggcgaag cagagcacga cgcacgccgt
     5161 ccccagcagc accgactccc acagcggccc atccttgcgc agcgtgaacc aatcgcccag
     5221 ccagcctcgc gaaccggttc tcgccggctg cggaggaggc gctgaggtgg cagggacgct
     5281 cataacagaa tggcagcgac gtctgacgtg gcaacgccgc tggatttaac gcaaagatgc
     5341 aaaggtgcaa tggcggagag aagtcaccga agcgaatgag gacatctcta aatatctgcc
     5401 ttctctttgc gcctctacga ctttgcgcct gtgcgtcaaa acgcaaggcg caccccaagg
     5461 tcccgcgccg agcgcaggga ttagtttgcc tccgccgtga agatcttgac ctcgacgcgg
     5521 cggttctggg cctgattgag cgggtcgctg ggatcggccg gcctgtccca gccaaagcct
     5581 tcgacgttga acttgttgaa atcgaaattg aacttcttga tcacctcttc cttcacggcc
     5641 ttggcccggt tgagcgacag ctccttgacc atctcggccg gaatcttgtc cttcatcgag
     5701 ctgtcggtgt gcccctcgat gattatccgg gcgttgccga actggccggc cagcttgccg
     5761 atctcatcga tcacatactt ggccttcggg tcgtaatcga cctcgatcgt cttgccctcc
     5821 tggtccttcg tgaccttctt gaagatctcc cagctgttgg gatagaagtg gatcacgacc
     5881 gtgttggtca ggatctcctc ctccccgcgg accgcggccg tcggcttggg cgcgaactgc
     5941 acgccgtact cgtctttctg cgcggcgtac ttcggctccg tgccgagctt cgcgatgatc
     6001 gagtaatcca tcacctggtc gaacggcacc tgctgattgc tcagcagccg caccgagcgg
     6061 tacaggtaat cggcctggtt ccagacccgg gcgaagtttg tggggttgtt ctggttcacg
     6121 aagaactggt agttctcgcc ccagttcgtg ctgtgggcgt cgcccagcat gcccagggcg
     6181 tccgccggct gcaggccgta gcccgtcgcc atcagctcgg cgcacttctg cttgttggca
     6241 tcctgcttga ggtcggccat cgcgtcgaag atgccgcgga ccaggccttc gcagatgtcc
     6301 ggatggtcct gggcaaagtc ggcccgggcg taccagacgt cggcgatcag cttgttggcg
     6361 gtgccggtcg tgaccagcat ccggttcccc ttcacctccg acagcttgta gatgtccggc
     6421 gcccacgaga ccgcggccgc gatgtccttc ctggtgttga acgccgcggc cgcttcgaac
     6481 gccgtgggcg tgccaaacat cttgacctcc gcgggctgca ggccgccggc gaccagcatg
     6541 ttcagcagga aatagtgcga cggcgaattc tcggccaggg cgatcgtctt gccccgcagg
     6601 tcggccaccg tcttgatcgt gtcgcggcag acgatcccgt cgccgccgtt ggaccagtct
     6661 acctgctgat agacgcgggg catgatccgg ctgtcgcgcg gattgcccga gctatcgacg
     6721 aagccatcga caaacagcgg cagcatgtcg agcgtcgccc agccgatgtg cacgttgccc
     6781 gccgtgtagc tgtcgcgcat ctggatcgga tcgtccatca gcacgagctc gacgcgaaaa
     6841 tccttgccgt cggccgtctt ccaggccttg tcggccttga agccgttgtt ggcatagatg
     6901 atgggggccc agccggccca gacgttgagg gcgaagcgga ccgtgttgtt ctccatcggc
     6961 ttgtaggccg cggttccctt caccgcgggc agccgctccg acggcttgtg cgtgaactcc
     7021 ttgaccgtcg tgatgccggc ggtgtcggcg gcttcggccg caccggtggc cggcgtgccg
     7081 ccattgggcg taccgccgtc gggtttgcca ccgttggttt tttgctcttg attcccggcc
     7141 tgatgcatcg gcggaacagg ccccggctga ttccgcaaca ttcgccaggc ggagaacgcg
     7201 atcagtcctg tgacgaccag gagaacggcg acgtaaaacg gggttttggg ttgtccggcc
     7261 atgttcgtgt cccctctcgc tttgggagag ggctagggtg agggtgtatg gttcttgtcg
     7321 ggcgaggcga atccagaaga cctgaagacc ctcgcccccg ccctctccca cagggagagg
     7381 ctgacgcatc agccgcgaaa cttcgcgtgc ggccgcgctt gctcgagcga gttcagcgtc
     7441 tgcatgatgt tataggcggt cagctcgcag agcagcttcg tgacctgctg gtgctgcgcg
     7501 tcgctgagcg tcggttcgac ctctttcatg acggctgaga gccgctgctc ctgcgccgcc
     7561 agctcgtggt cgtcgatctt gccgtcggcc atcgcctgga cgaacgtcga gagcttctgg
     7621 acataggcgt cgatgaccgg cgtttgggcc ttttcatcga gccagctctg tttgcctgta
     7681 gccatagatc gctttctccg tgattcgccg cttgcggccg attattgtgg caccggccgg
     7741 cgcaaattgg gagagggcga aaacggagcc gccgcgcgcg ggcagacttc ccgcgcgacc
     7801 aaagcgtgct gcaatgccgt ccacggccag gcaaactgcg gcgcaaactc ccgatcaggt
     7861 cgccgcggct gcggaggcca agtcggggat ctccagcagc tcctggagcg catcgacgct
     7921 gatgggcttg ctgtagtgca agtcgaatcc ggcgtcggtg gcccgctgct tgtcttcggc
     7981 gcgagaccag cccgtcaccg cgacgaggat cgtgcgcccg ccggtcgagc ggcgaatgcg
     8041 ggcggccgtt tcgtagccgt ccatgccggg catgccgatg tcgatcaggg ccagttccgg
     8101 ctgctgctct tcggcagcgc ggatcgcctg cgccccgctg taggcgacga tcggttcttg
     8161 ccccaaaatc tgtaccagtc tggcgagtga gtccgcgttg tcgtggtcgt catcgacaac
     8221 aagaatccgt gcgctcatcg caatctttcc tcacggtcga gtttcctgtt ccggttagca
     8281 ggccgaacgg ccctgggaga ggccttcaat ttcagccaga ttatttttga ttacaaggcc
     8341 acacacccga aaaatcgggc agattcaaga aaatggcgat ctggcgtact agcgtgacaa
     8401 agatgctagc actgtcgcgt ggtggacaac ttgtcggaag ccggcctcgc aggccgggaa
     8461 ccccctaaag ggctcagccc ggcagcaccg aggcgattcg tgaccagccc cgagcgcttt
     8521 cgtcccacgg cgctaccggc aggtctcgat catcctccgg ccactccaaa ctcgtccggg
     8581 aatcgctcga ccaggttttc ttgtcgtcgt cggctgattg gccagtcggt ggacgaagag
     8641 aacccaggtc ggtggattcg ccatcatcgg ggaaagctgg aatcatggtc gaaaagccaa
     8701 gatgtcgctg tccgaggcag caatagccca ggcgtcattg ccggcgtcca ataatgcatt
     8761 cggcgtgccg cgccagaatg gatgccaaaa aaactcagcc gtcccgcagg atttcctcgg
     8821 cggcgttgcg gccgcaggcg ccgaggacgc cgccgccggg gtgggtggct gcgccgcaga
     8881 gatagagccc gcgaatgggc gtgcgatggt cggcccagcc gggcacgggc cgcatcatga
     8941 atagctgctg gggcgtcatc gcgccctgca tgatgttgcc gccggtcagg ccgatcagcc
     9001 gctccaggtc cagcggcgtc agcacctgcc tgtgcaggac ggcgtttttg atgttcggcg
     9061 catagcgagc gagcagatcg agacagcggt cgccaaactc ctccttgatg tcgtcccagg
     9121 actggccatc ggccagtttg tacggcgcat attgcacgaa gatgttcatc acgtgctggc
     9181 ccggcggggc gagcgtgtga tcgacgctcg tcggcagcgt gatctccagg atcggctcgc
     9241 tgctggggct gccgtatttg gcgtcgtcga aggcccgctc catgtagtcg agcgtcgggc
     9301 tgatgtggat cgtgccgcga tggtgcggcg cgatcacagt ggacggcaga caggtaaagt
     9361 ttggcagctc cgagagggcg acgttgatct tggccgacgc cgaggcgtaa tcgattttgg
     9421 cgaccgccgt gcggaattcc gcgggcagct ctgccggatc gagcaatcgc tcgaacgtct
     9481 gatgcgcgtc gatgctggac gccacgatgg gcgcttccag cagcgaatcg tcccacagcg
     9541 tgacgccggc gacctggccc ttgctcacga ggatccgctt caccgacgac tcgcggcgaa
     9601 tctcgacgcc caaatcgtgg caggccttct ccagcgcggc ggtcagcccg cccatcccgc
     9661 cttggacata gccccagact ccgcggctgc cgcccgcttc gcccatcacg tgatggagca
     9721 ggacgtacgc cgtgcccgga tatgacggcg aggcgaacgc gccgatcacg gcgtcagtgg
     9781 ccagcgtcgt ccgcaggact tccgcctcga accaccgctc cagaatcggc cgcgccgggc
     9841 cgatgagcag ctcgatcgcc tcagggaggt cttcgccgag ccggccgatc gactgataca
     9901 gctccagtaa gcggccggcg tcgcgcaggc gcttgccgac gccgatcttc cgccactcgg
     9961 cgggcagcgg cagcggatcg ggggcggcct tggccaggat cggctcgagc accgcggcca
    10021 cgcgctccag cagccggttg taggccggat agcgctcggc gtcctgccgg ctgaacttgg
    10081 cgatctcgcc gcaggtcgcc cgctcgtcgg ggcccatcaa gagcgagcgg ccatcgagca
    10141 gcggcgtgaa cgacgaggga ttccgcggca ggatactgag tccgtactgc ttcagccgca
    10201 gctcgcgaat gatctccggt cggaagaggc tgatgacgta cgacgcgacc gagatcttga
    10261 atcccggcca cagctcttcc gtgaccgcac agccgcccag cacgtgccgc cgctccagca
    10321 cgcaaacctt cttgccgccg cgtgccaggt aagccgccgc caccagtccg ttgtgtccgg
    10381 cgccgatgat gaggcaatca taccgttggc tcggttttga catggcggga ttatagtaga
    10441 ccactcgcat tgctatgttg ctcttgctcg ttgtcagtac cccggccgag tccggacttt
    10501 ccgtcatggc ggtcatcctg gtacctgcgt cgggcgatga cctgacgatc aattggtgga
    10561 actggcgtcc caccgtagcg ctgttagttc gtgcgggcgt attgccgccc ggtgaacgag
    10621 cagaccgctg cgagtcgaat gggtgcgggg gttacctctc cgcgaccgag gcgattcagg
    10681 cggccgacca catcgaatcg ctggttgccc gaatggccac caacgagcgg ctcctgtctc
    10741 gcggtgagat cgtcgcggtg cctgagtccg acaagccggt ttccgagtgg accgaagaag
    10801 aggcttacca gcgctacgcc gctcggcgtg agttgctcgt tccgttcgcc gatttctgcc
    10861 ggcgttcagg cggttttcaa gtcgtgtaga aaagatgttg aggcctttac tgatgaaatc
    10921 tccagttgtt taccttgccg ctgcgtggag tttggccttc ttcggaagcg cgtttgcagc
    10981 cgatcgccca ccaaatttcg tcgtgatctt cgccgacgat ctcggctacg gcgatttggg
    11041 ctgctacggc catccgacga tcaagacgcc gcacctcgac cgcatggccc gcgaggggct
    11101 gcggttcacg cagttctatt cggcggcgga agtctgtacg cccagc
//
LOCUS       DASRJB010000181        25489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3153989, whole genome shotgun sequence.
ACCESSION   DASRJB010000181 DASRJB010000000
VERSION     DASRJB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 25489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25489
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153989"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFB80_05010"
     CDS             <1..1101
                     /locus_tag="VFB80_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HZN33152.1"
                     /translation="VARFARFRHRAVIEAGRLEEVELEANDSGGAAVLLYDGPWFTLT
                     STAGDEPTRRCVVRIEQMFRAYRTLLPPRVQQPQRLSVVLYGSLDDYRQRLRRLDLAL
                     DNAAFYWPKERTIFAGSDLNLFAQRLSQVRREHEQVRQAYAKLDAQHDAAMNALNAEL
                     KAAGFSAADATAEIRQRRATWKAEMDEALTANAERQRTNERKFADVTGQMFTRLTHEA
                     FHAYLGTFVFPPDEQHVPRWLNEGLAQVFEGGQLDGDSLRLDAPDRERLKRLRADVAQ
                     QPLPLAQLLTAQEREFLGPHGTESSQRHYLYAWGLAHYLTFHESLLASPRLGEYVTSA
                     AQRMDPIERFEKLAGRPLAEFERQWRAAMAPP"
     gene            1257..4310
                     /locus_tag="VFB80_05015"
     CDS             1257..4310
                     /locus_tag="VFB80_05015"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33153.1"
                     /translation="MKRLLLVCAILFIPAAALAADSAGVRSQLFDGKSLDGWVVTGCE
                     AVVDGDVLLAKAGDGLVRTENRYRDFVLELDYRALQKEKYDAGIYIRCEPPPAGRPFP
                     AQYQVNLKQGDELNLIRFPKAKSTGLVKPNDWNHVKLTVVGERAAMEINGQAAWETDG
                     IQAKDGYLGLQVEVPLGGQFEYKNIAVTELDYRPLFDGTSLAGWEGAGQPAEKCWKAE
                     EGLIVCTGEKGPWLRSAEQHGDYNLRLEYKLKAGGNSGVYIRVPENGNHHGNGAGIEV
                     QVLDDADGRYRTIRPYQYTGSLYAIAPATKHVARTAGQWNRLEINCLGTKYHVFHNGV
                     LIVAADETTFPELKGRLTKGFLGLQNHSEEVWYRNLRIGPPLDLPIDPSSKQTTLKAE
                     LIRELLSQPILPPGTPQREVEAFCEARVVRLPAADAPERKSAEAWTAYVEGLRKKTLD
                     EVVFRGEAARWRDYQGKVEWLETIEGGEGYQIKKLRFEAAPGLWVPGLLYEPTKLDGK
                     TPVFLNVNGHDGKGKAADYKQIRCINQAKRGIINLNLEWFGMGQLRGPGFTHYRLNQL
                     DLCGTSGVAPFYLAMKRGLDILLAHPNADSSRVGVAGLSGGGWQTIFFSALEPRVTLA
                     NPVAGYSSYLTRIHNYSDLGDSEQTPVDLAATADYAHLTAMLAPRVALLTFNEKDNCC
                     FAAPHALPPLLEAARPVYQLLGRAENLHDHINYEPGTHNFLRDNREALYKVIGQHWYA
                     GDAAFSPNEIECEAEVKTAEELTVELPTDNLDLQQLAGRLMQPQPKSAEPAQQREALT
                     RALRTGKHQRLPLEYSIGKGEFSRQMAGAVEVTSWQLKIGEAWTIPAVEIAPPESKTV
                     AIVLADAGRADAAAAIEKLLAAGNRVVAVDPFYLGESKIKNKDFLFALLVSSVGERPL
                     GVQAGQLIAVAKWLSGPRQLGPAKIEAVGPRTSLIALAATALEPEAIAGLALHGSYGS
                     LKEVIEKGGQVNATPEVFCFGLLEAADIPQLAALAAPRKVEFLEK"
     gene            4325..5566
                     /locus_tag="VFB80_05020"
     CDS             4325..5566
                     /locus_tag="VFB80_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33154.1"
                     /translation="MNFARYWILLVCPLFAAADWPQFRGPAGDGHGSARNLPATWSET
                     KNVAWKTAIPGKGWSSPVLVADKLYLTTAAPLDPGSASRGELSLRGLCLDAATGKILW
                     DKEVFRQDAGAPAIHTKNSHASPTPLVTGGRLYVHFGHQGTACLDPAGNILWQNRKNA
                     YEPVHGNGCSPILVDGLLIFSCDGATNPYVVALRAANGQEAWRFSRPGDPPKKFSFCT
                     ATLIEVDGQKQVICPAAGVVNALDPASGREIWRVTTEGYSVIPKPIFGQGLVLLSTGY
                     DAPEFVAIRPTGRGDVTNSHVAWTAQRGAPHTPSPLLVGDEVYLVSDKGVATCLDVKT
                     GREHWQKRVVGNYSSSPVFADGKIYIQSEEGPAVVIAPSKEFTKLADAGFQERTLASY
                     AVGDDCLFIRTEKNLYRVQGW"
     gene            5592..6599
                     /locus_tag="VFB80_05025"
     CDS             5592..6599
                     /locus_tag="VFB80_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN33155.1"
                     /translation="MNHRLLGRTGVRVSPLCLGTMMFGAQTTEADSIAIIHKALGLGV
                     NFIDTADMYVAGESERIVGKALAGRRAGVVLATKGRNKMGEGPNDWGTSRLHLRQALD
                     ASLQRLATDYIDLYYVHAPDYNTPIDETLRFLDDAVHQGKILYTACSNFRAWRVCEAL
                     WTSDKLGLASFAAVQPLYNLVNRDIEVELLPLCQARQIGVVSYSPLARGILTAKYKPG
                     EKFPEGSRASRNDKRMKEAELRDESLIISQKLADHCRSKGVAASQFALAWCLANPILS
                     SIILGPRTMEQFDDNVAALNVTITPADEAFVDSLVPPGEHSGKGFQDPAYPVLGRPKT
                     I"
     gene            6666..8777
                     /locus_tag="VFB80_05030"
     CDS             6666..8777
                     /locus_tag="VFB80_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33156.1"
                     /translation="MSKRLLNIVACVVVGLVVVTLGVIIWQQSQHPNDYSEVIDFPAP
                     PPPKVSATKPAPQPPRGPNPPAPLPQVKKQPEPDEPPQPAAEPVRKGDLTWSQVEEAF
                     ERVRRARKEKPVLNHDLSSMYDDVAAPAYTDSADYPAHCAALAKWRDEFPQSPTPLIA
                     LAKSHIDYAWQARGGGYAFTVTDDGWQLFHTRIDEARRLLEKAAKLDLKDGEAYRQLL
                     IVAMAESLPDEQVRAWFDAGRKIDPGYAALYTQMATSMMPRWGGAPGDVERFAAEVAE
                     LVPGEDGLELFGLIAREIHRYECDDPTTILWGDYDRRLLAKSADVLVARYPQAQSVVQ
                     FAALCSQVCQDHELARRIRPLVGPLNANDRVWIWKNSYEDFIEWAEAKEIPGGEESSV
                     WAALAGCVDFSFADDSRYLWAGHQFGRRSVTLFDLQTKKIRLELPHPGGVVNHALFDS
                     ASKQIVVSAWRGPLVGWMLFDLGDSAEPLVFTTPQQVTAIALNPRQKQLAWVEAKGGL
                     RLLDLTAEDRTPRDLKVLVHTLKFSGDGTRLAAGAAEWFIFDTASGEQLLTLPSIRKP
                     QQGMWCDQVLDIDDSGRIWATARSLGKPPPRRALIRWSANGEQPETIIADIGEGMVKL
                     SPDRTLLAVVRDSEVNGGPSSVDVLDVGKGQRVKQLAGHWNAIGDLRFSADSKKLATI
                     ARLSDVIKVWSLDETAAAVKE"
     gene            complement(8827..9951)
                     /gene="menC"
                     /locus_tag="VFB80_05035"
     CDS             complement(8827..9951)
                     /gene="menC"
                     /locus_tag="VFB80_05035"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZN33157.1"
                     /translation="MQIDRIDLFHVAMPLIYPWRTAYGEDAAIESVLCRMASGSCVGW
                     GETSSLAAPCYSPEWAGGVFAVAKQWLAPALVGQQIASGEQLQQRLTHFKGNQFAKAA
                     LDTAWWSLQSQRAGKPLHELLADAAGCTARADVPVGADFGVMDSIDDLLVAAGKAVAE
                     GFPRIKLKFRPGWDLPMLRAVRREHPSHTFHIDTNSGYRLADLPLFREVDELNLAMIE
                     QPLAHDDLVDHAELARQIRTPVCLDESITGLRQAEQAIALKSCRYVNVKPGRVGGLTV
                     AVQIHNGCSRASIPCWVGGMLESAVGASHCVALAMLPNFTYPADIFPSSRFYREDLAE
                     PPLELIRTAAGQPGVQPFLRLPEPHPQRLEKLTVQRATLA"
     gene            10166..10720
                     /locus_tag="VFB80_05040"
     CDS             10166..10720
                     /locus_tag="VFB80_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN33158.1"
                     /translation="MHDLVELRTERLLLRRWRAADREPFAALNADPRVMEHFPALLSR
                     EQSDERVERIEAHFQQHGYCQWAVEIPGVTPFAGFIGLSVPQFEAHFTPCVEIGWRLA
                     ADCWGRGYATEGARAALPFGFEKLALAEIVSFTVPANVRSRRVMEKLGMTHDPADDFD
                     HPLLPAGHPLRRHVLYRLRKNSAG"
     gene            10732..11109
                     /locus_tag="VFB80_05045"
     CDS             10732..11109
                     /locus_tag="VFB80_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33159.1"
                     /translation="MYWRSLIRFRLLTLLLLVAVLGALLVANTRQHSHRTLGHVSGDA
                     PPIFPGLWIFGHDYGWPFPYKTESFKTDDFRIDVFDGFHWLGLVGDSLFGLALLLAAG
                     ALSELSLWALRRITGFRRKLKRN"
     gene            11430..13853
                     /locus_tag="VFB80_05050"
     CDS             11430..13853
                     /locus_tag="VFB80_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN33160.1"
                     /translation="MELAPNGDLWVLEQTGAVKRFRPGSTTADIVGSVSGLGLNSSGE
                     RGLLGIAFDPQYATNKQVFLYYTTTSPFLHNRISAFTVVDTSAADYFFAGTSTTPADD
                     GSTGVPTQTVIFDLDALSGATNHNGGAIHFGPDGKLYAAVGDNANGSNAQTLANLHGK
                     MLRINKDGTIPSDNPFFSSATGDERAIWALGLRNPFTFTFQPGTGRMFINDVGEVSWE
                     EINDGIAGSNYGWPQNEGNPTVAPPGPGTHRGPIYAYSHGGGTFQGFAITGGAFYNPT
                     TPQFPAEFAGDYFFADFVNDWINVRDAGGTVTRFASGAQGPVDLRVAGDGSLYYLARD
                     GGQAFRVTFPQAPAGNPSALYRFNETSGTVAADASGNGQSAQYVNGPALGQPGVVAGG
                     TAVSLNGVNQYIAVPDAPLGAYGSPLSFETWFNAPAGAGGTILGQTAAGGVPGGSIPP
                     GYVPVVHLGTDGRLRSSLFWHGSAGARLASPAGQSYNDGNWHHVAVTYVAGVETLYID
                     GAQVAQQTISQAGYATDYDYFIGVGQTSLWEAGNGGWHFFRGRLDEAVFFQRALTPTE
                     VAQHFAQGNPPPVNSAPIATYRFDEASGITAIDASGNGNSGMYVNGPALGQPGALPAG
                     TAVSLNGTNQYVALPDAPFGTYGGPLSFETWFNAPAGASGTILSQLGGGTTVGGAATR
                     GYVPVVHLGVDGRLRSSLFWHGSATQRLTSPVGAVFNDGNWHHVAVTYAAGVETLYID
                     GVQVGQQTINQVAYAADYDYYLGVGFTDLWAAGNGGWHYFRGRLDEASLYQRALTASE
                     VAQHFAARA"
     gene            complement(13855..16752)
                     /locus_tag="VFB80_05055"
     CDS             complement(13855..16752)
                     /locus_tag="VFB80_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33161.1"
                     /translation="MLIARGGRAGEPYLEFLRALQLKGYGEQALAYLDQIADQPDLPA
                     DLKRDLVLERSKSLKIAATEAYDAPQRDSRLAEAKKLSEQFFKDHPDHAEAASTLLDE
                     GKAAQFRGQISLAKMRAARSADEQKEHRTAARTTLTEARKLFADAEKTLKARLDALPA
                     DRPVRGRNNPRELLDWAWLEARFYGAQCVLFAAQAIENAKDKERVQMLKEAGDALDKL
                     FQEYRGKPLGSLAHLWHGLVYEEQGNTTTALDVYDEVLVLAPEGKDADLDLAPLFGQA
                     ALFRLKLIAKTAEPKKVLAEGEEWLLTNKRWQSTGPYQGMHLETTRARLKLVESARTA
                     TEKQKLTRDAIAALTAIGKVDSEYRHEATMMRRGIVENTTASANMTLAERIAVGDEAA
                     ASGNWSSAETMYREAITQATTARDNKQVTAVKGKLAQVLYQRVLKHYSAGEMDQCLAL
                     AGEIVRDNSDQPIAESTSAVAVAAALQVYSTVVADGKAAALARLEKVATYAISRWPDN
                     PVGDDARMALAQARLIQGDFAGVEQQVAAVTPQSKRYPTALQILGQIRWRQYVDIKKS
                     EDPARAEEGKKHRAEAVSALQTSVQRQRAAWQAGTEPMPEPLFDTQLLLAEIMLEGQQ
                     FKDSAELFAPLMKALAEKPPGAIDQKTQRAYVGAVRALLAVGDAAQAGEAATAFASIS
                     GDSAQPNSLLVDLSKLFAIELRKLEAESETGVSGTPMNTAPPPAIGNPPPSAPPADPH
                     VAALRDSLGKQIDAIYGRNLLSVPQLIYVGDTSVLLGKNEQARTVYQRLLDTIDKDES
                     AKATAGAATTRIRSRIIGLLRSEGKLVEANKQVDALIKAHPTALEPQLEKGYILESLA
                     AAKPAMYDECIAHWTQLRVQLGRAKTRLPEYYEVLYNAARCLVRQARATNNKEKALQA
                     EQMLKSTLTLSPKLSGPDMVAKYEALLKAAVILRGAPTAAASSEPRRAAVP"
     gene            complement(16816..18459)
                     /locus_tag="VFB80_05060"
     CDS             complement(16816..18459)
                     /locus_tag="VFB80_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN33162.1"
                     /translation="MIKTNKPAPAAAPAEQASQASAAEHPEEVASGSEGWMKDLMVGI
                     ATQSWLASMAIHMTLFIILALVLGTIHVAATIGAAPVFEVIEEPQSLEPEITHFEVGY
                     TPTDPTELTTETLTMTEAPSVEAQFNDNSPIFTEAGGGIVGGDSNFGGLGGFNVAASG
                     LGPIVKGGGGVDAGAGFGKNMGTGGAGTGFGSRGTGMREAMLGTGGTKQSERAVAAAL
                     NWIARHQSPDGKWSLNHTPMCRSGFCSGPGGANSDGAGTGLGLLPFLAAGQTHESKGP
                     YQKNILAGINFLIKNQKKTGDLSVNGSQMYAHGLATIALCEAYGMTQDSRIGYAAQAA
                     IDFIQSGQNRDGAWRYQHGTEQSDTSVYGWQVMALKSGQMAGLNVNPASLQGAKQYLS
                     KVSLGQYKSEFSYTPGGGPTFTMTSVGLLTSQYLGARRDDPVIIGGVEYLSKRKPSVN
                     QRNTYYWYYATQVMHNVPGAQWDDWNRTMRRILIDTQEKSGCQAGSWDPDKPEPDPWG
                     NQGGRLMVTSLSCLTLEVYYRYLPLYKIDDVKKDLNKAQ"
     gene            complement(18486..19526)
                     /locus_tag="VFB80_05065"
     CDS             complement(18486..19526)
                     /locus_tag="VFB80_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33163.1"
                     /translation="MRAAAARPAGALRLISMLAMLAVLAMLIARAADPGTWVWLTGET
                     AAEVTAAKGTATKEADSKPPAAVSPAAAAAAPEQVVDGTDLDPDERATAQEQFQALSD
                     FTLDMGREEMPAYWRLFRWSKNQPLAQLEQRADKNVLFNQFIDRNTRDAQRGKLFRLD
                     LTVRRILSYPAAENSAGVEKVYEILGTTDQSQAWFYMLLTDELPPGMPEGARIHERAT
                     FVGYFLKVQGYQHAGAGPTDKPLPAPLLIGRLQWRPAQVAAAQSDWEFLWKAKNSPWV
                     WWVVGTVAVFCIGRLALWLYRRSRPIKAEFADDGKIPPHKAGAVRDWLDSVEEENEAA
                     ARGIRNADYQNN"
     gene            complement(19569..20072)
                     /locus_tag="VFB80_05070"
     CDS             complement(19569..20072)
                     /locus_tag="VFB80_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN33164.1"
                     /translation="MRRRKKRRSQGEVELNLAAMLDMAFQLLTFFILTFKPAPVEGQV
                     LLRMPPPQPITNVNAGQKAGSDASNTNPVQGLNTLVITAIANPDGGLRQMAVGDATMG
                     SVPALERKLSTVLGDPGSGFDQVIVQVDSRLRYDELMQIIDVCTRQKLPNGEVLSKLS
                     FVELPAG"
     gene            complement(20075..20542)
                     /locus_tag="VFB80_05075"
     CDS             complement(20075..20542)
                     /locus_tag="VFB80_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN33165.1"
                     /translation="MSASLSHSSSAEPNLTPILDMVFQLITFFMLVINFKAASMDLDL
                     KLPVIGSARPVDTQGQEDLLILNINSQGQLRVYGQLIPDISTYIANEATASLMVARQT
                     NPNLKAGDELPSTVVIRADKSTAFAMLNRVISKCQEHGYRKFALKAMNKTEET"
     gene            complement(20614..21432)
                     /locus_tag="VFB80_05080"
     CDS             complement(20614..21432)
                     /locus_tag="VFB80_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN33166.1"
                     /translation="MAVWNRWIVALLMFSAVFTITQADLPRAAAQEPAAAPAAPAGAP
                     AAPAPKPKSRFVWFIMSSGLIGLFILILSIYFVATVIRLFIELRLDVAMPTEVVAQCE
                     QLLANRDYQGLYDAANHSSSLYGKLVSVGIAELPSGLAEAREVMDRNCDAETVDMERK
                     ISMLAVLGTLGPMIGLVGTLKGMIASFAVIALSDTQLKASEVAGGISEALLLTFEGVA
                     LSVPAIYFFAVFKNRVAKISVETLLAADELVRRLVQQAKKPGAAPAAQTHPVRG"
     gene            complement(21626..23758)
                     /locus_tag="VFB80_05085"
     CDS             complement(21626..23758)
                     /locus_tag="VFB80_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZN33167.1"
                     /translation="MASSVAPSGVPVSRRQPTDVEELTDYEEIPDGEPAGEAGESPVG
                     VPAAGELAGDDDLDAAEARREWMRSLTCTLASAGVHMAVFLVLALVLGTITMPKPPPI
                     AFDVEEWSRQEELLKAELEPTLTLAYTRPEALYSSASIASAAATAVDVAGGGGGTGGG
                     GGGSGGPSGGMIGGKGSGRGGSGLQLDGSLFSSGVGSGTGGIGGIELNLPGGRKLIDA
                     VPDGQIGDSRAIVGGYQQALDRLTQEILWMLDKGPVLTMWAFDQSESMQDDQKEIRER
                     IEHVYRQLGLLRSNNSGALETAVTSYGEKFLVHTRQPTHDYDEIRAAIAEVPVDKSGI
                     EMMCSAILQTVAIHKQYAQRSRRQMALIVVTDESGERSDNDQHLEQVIAEAKAARCKI
                     YVLGREAVFGYPYAHIRWVHPQTRNTHWIRIDRGPETAFVEQLQTDGFHRRYDAHPSG
                     FGPYECTRMSQETGGIFFMLPSLETNLVRGQKGNYDLEALQPYLPDVRSRQEVKHDID
                     NSPLRTMLQKVIYDLNPYNPVAQKIIEMRVEFARDPQAFVRQARQEQAKAIVYIQYLE
                     KAQQEVEKLQYMRSHEALPRWQANYDLLHAQIIAYQARMYEYGAYLEEFIKNPKTAPL
                     TRPPNLTHVTWDIRTRHEIITGAKVQPYIERATAMFKKILVDHPGTPWAGRAQYELNR
                     GFGVELIPDYDAPNPYTGSTPLVPVPKM"
     gene            complement(23954..>25489)
                     /locus_tag="VFB80_05090"
     CDS             complement(23954..>25489)
                     /locus_tag="VFB80_05090"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33168.1"
                     /translation="AKALAHFQEAVRVRRQVLGPNHSATMQAEDRLGSALRQTGKTGA
                     AIQLHEELLARCQRVKGADSQSALAVEFSLVQDYQADGQPAEALRRSEHLVQRSLAVF
                     GTEHESTLKVQHQRGTLLSQAGQWRQALPILEQVVAARTKLYGPHDPLTLGSLANLAS
                     IYRKQGRRADARKQFEDNLEQISARLGPQHPRTLTAKTGIAQMLAEEGRFQDAIDMAS
                     AAHQTAAAKIGPAHPDTLEIAAALAEIYESAGRVADALSIAEETYRLRKETLGLEHPS
                     TLQAQSNLASAFRLSGREGDAVRLTEESLAGLRKKLPPNHPKIVWALNNLGLAYKEAG
                     QPEKALPLYQEVVQSLTERYGPASANTLTARNNLGGALMNLNRVGDAIDLCEETLAIC
                     DEKLGADDQTTLIVVHNLGTLYHAADRSQDAERLFRRAFEGRQLRLGPNNADTLQSLY
                     RLAGIYLLRKDLAQAEPLLLDCHQRLKASPQAPADLVKNVETALAVLAKVKADPTLTP
                     KRK"
BASE COUNT         4267 a         8355 c         8313 g         4554 t
ORIGIN      
        1 gtcgcgcgct tcgctcgctt ccgccaccgg gccgtcatcg aagccggccg gctggaagaa
       61 gtcgagctgg aagccaacga ttccggcggc gcggcggtgc tgctttacga cggcccctgg
      121 ttcacgctga ccagcaccgc cggcgacgaa ccgacgcggc gctgcgtcgt gcggatcgag
      181 cagatgttcc gggcctaccg cacgctcttg ccgccccgcg tccagcagcc ccagcggctg
      241 tcggtcgtgc tctacggctc gctcgacgac tatcgccagc ggctgcggcg gctggacctg
      301 gcgctcgaca acgccgcgtt ctactggccc aaggagcgga cgatctttgc cggcagcgac
      361 ctgaacctgt tcgcccagcg gctgtcccag gtccgccgcg agcacgagca ggtccgccag
      421 gcgtacgcga agctcgacgc gcagcacgac gccgcgatga acgccctcaa tgccgagctg
      481 aaggccgccg gcttctccgc agccgatgcc acggcggaaa ttcgccagcg gagggcgacc
      541 tggaaggcgg aaatggacga ggccctcacc gccaacgccg agcggcagcg gaccaacgag
      601 cgcaagttcg ccgatgtcac cgggcagatg ttcacccgcc tgacgcacga agcgtttcac
      661 gcctacctgg gcacgtttgt cttcccgccg gacgagcagc acgtgccgcg gtggctgaac
      721 gaaggcctgg cccaggtctt cgaaggcggc cagctcgacg gcgactctct gcggctggac
      781 gcgcccgacc gcgaacgctt gaagcgtctg cgggcagacg tggcccagca gccgctgccg
      841 ctggcgcagc tgctgaccgc ccaggagcgc gagttccttg gcccgcacgg cacggaatct
      901 tcgcagcggc actatctcta cgcctggggc ctggcccatt acctcacgtt ccacgagagc
      961 cttttggcca gcccgcggct gggcgaatat gtgacgtctg ccgcccaacg catggacccg
     1021 atcgagcggt tcgagaagct tgccggccgg ccgctggccg agttcgaacg ccagtggcgc
     1081 gccgcgatgg cgcctccata acaacccgca gcgtaagcga gggacttatt gggcgccgaa
     1141 tttcaaaaag tccctcgctt acgcgtcggg ttggtgattt gaggatgctt gcccgccgct
     1201 gacgcgcgag cgtatcatcc actctcccgc ccgattttcc cgccgaggtt gttcccatga
     1261 agcgcctgct gctcgtctgc gcgatcctct tcatcccggc agccgcgctt gccgccgact
     1321 cggctggcgt ccgcagccag ctcttcgacg gcaagtcgct ggatggctgg gtcgtcacgg
     1381 gctgcgaagc cgtcgtcgat ggcgacgtct tgctggccaa ggcgggcgac gggctggttc
     1441 gcaccgagaa ccgctatcgc gacttcgtgc tggagctcga ctaccgcgcg ctgcagaagg
     1501 agaaatacga cgcgggcatc tacattcgct gcgagccgcc gccggcgggc cggccgttcc
     1561 ccgctcagta ccaggtcaac ctcaagcagg gggacgaact gaacctgatc cgctttccca
     1621 aggccaagag cacgggcttg gtgaagccca atgattggaa ccacgtcaag ctgaccgtcg
     1681 tcggcgagcg ggccgcgatg gagatcaacg gccaggccgc ctgggagacc gacggcatcc
     1741 aggccaagga cggctatctc ggactgcaag tggaagtgcc gctgggcggg caattcgaat
     1801 acaagaacat cgcggtcacc gagctggatt accggccgct gttcgatggc acgtcgctcg
     1861 ccggctggga aggggccggg cagccggccg agaaatgctg gaaagcggaa gagggtctga
     1921 tcgtttgcac gggcgaaaag ggcccctggc tcaggagcgc cgagcagcat ggcgactaca
     1981 atttgcggct ggaatacaag ctgaaagccg gcggcaacag cggcgtctat atccgcgtgc
     2041 cggaaaacgg caaccatcac ggcaacggcg cggggattga agtccaggtg ctcgacgatg
     2101 ccgacggccg ctaccggacg attcgcccgt atcaatacac cggcagcctc tatgccatcg
     2161 ccccggcgac gaagcacgtc gcccgcaccg ccggccagtg gaaccggctg gagatcaact
     2221 gcctcggcac gaagtaccac gtctttcaca atggcgtgct catcgtcgca gccgacgaga
     2281 cgacgtttcc cgaactgaag ggccgcctga ccaagggctt tctcggcctg cagaaccaca
     2341 gcgaggaagt ctggtaccgc aacttgcgga tcggtccgcc gctcgatctg cccatcgatc
     2401 cgtcgtcgaa gcagacgacg ctcaaggccg agctgatccg cgagctgctg tcgcagccaa
     2461 tcctgccgcc gggcacgccg cagcgtgagg tcgaggcgtt ttgcgaggcc cgcgtcgtcc
     2521 ggctgccggc cgcggatgcg ccggagcgca aaagtgccga agcctggacg gcttacgtcg
     2581 agggtctgcg aaagaagacg ctcgacgaag tcgtcttccg cggcgaagca gccaggtggc
     2641 gcgactatca ggggaaggtc gagtggctgg aaacgatcga aggcggcgaa ggctaccaga
     2701 tcaagaagct gcggttcgaa gccgcgccgg gattgtgggt accggggctg ctttatgagc
     2761 cgacaaagct cgacggcaag acgccagtct ttctgaacgt aaacggccac gacggcaagg
     2821 gcaaggcggc cgactacaag cagattcgct gcatcaatca ggcgaagcgg ggaatcatca
     2881 atctcaacct ggagtggttc ggcatgggcc agcttcgcgg gcccggattc acgcactaca
     2941 ggctgaacca gctcgatttg tgcggcacca gcggcgtcgc gcccttttat ctggcgatga
     3001 agcgcgggct cgatatcctg ctcgctcatc cgaatgccga cagcagccgc gtgggcgtgg
     3061 ccgggctttc gggtggcggc tggcagacga ttttcttcag cgcgctggag ccgcgggtaa
     3121 cgctggccaa tcccgttgcc ggctattcga gctacttgac gcggattcac aactattccg
     3181 acctgggcga ctcggagcag acgccggtcg atctggcggc gaccgccgat tacgcgcatc
     3241 tgaccgcgat gctcgcccca cgcgtcgcgc tgctcacgtt caacgaaaag gacaactgct
     3301 gcttcgccgc gccgcacgcc ctgccgccgc tcctggaagc ggcccggccc gtttatcagc
     3361 tcctcggccg cgcggaaaac ctccacgacc atatcaacta cgaacctggg actcacaact
     3421 tcctccgcga caaccgcgag gccctctaca aggtcatcgg ccagcactgg tacgccggcg
     3481 atgccgcctt ctcgcctaac gagatcgagt gcgaggccga ggtgaagacc gcggaggaac
     3541 tcaccgtcga gttgccgacg gataacctcg acttgcagca gctggctggc cggctcatgc
     3601 agccgcagcc gaaatcggcc gagccggcgc agcagcgcga ggcgctgacc cgcgcccttc
     3661 gcaccggcaa gcaccagcgg ctgccgctgg agtattcgat cggcaagggc gaatttagcc
     3721 ggcagatggc aggcgcggtc gaggtgacga gctggcagct gaagatcggc gaggcatgga
     3781 cgattcccgc ggtcgaaatc gcccctccgg agtcgaaaac ggtggcgatc gtcctggccg
     3841 acgcgggccg ggccgatgcg gcggccgcaa tcgagaagct gctcgccgcc ggcaaccgcg
     3901 tggtggcggt cgatccgttc tatctcggcg aatcgaagat caagaacaag gacttcctgt
     3961 ttgctttgct ggtctcgtcg gttggcgagc ggccgctggg cgttcaggcc ggccagctga
     4021 tcgccgtggc gaagtggcta tctggcccgc ggcagctagg tcccgccaaa atcgaggccg
     4081 tcggcccgcg gacgagcctg atcgcgctgg ctgctacagc gctggagcca gaggcaatcg
     4141 ccggcctcgc gctgcacggc agctatggct cgctgaaaga ggtgatcgaa aagggcgggc
     4201 aggtgaacgc gacgcccgag gtcttttgct tcggcctgct ggaggctgcc gacatcccac
     4261 agctcgcggc gctcgccgcc ccacgcaaag tcgaattcct cgaaaaatag cccctcgaga
     4321 aatgatgaac ttcgcccgct attggattct gctcgtctgt cccttattcg ccgccgccga
     4381 ctggccgcaa tttcgcggac ccgccggcga cggccacggt tcggccagga acctgccggc
     4441 gacctggagc gagacgaaga acgtcgcctg gaaaacggcg attcccggca agggctggtc
     4501 gtcgccggtg ctggtggcgg acaagctata cctcacgacg gctgcgccgc tcgatccggg
     4561 cagcgcgagc cgcggcgaac tttcgctgcg ggggctgtgc ctcgatgcgg cgacgggcaa
     4621 gatcctgtgg gataaggaag tcttccgcca ggatgccggc gctcccgcga tccacaccaa
     4681 gaacagccac gctagcccga cgcccctcgt caccggcggc cggctgtatg tgcatttcgg
     4741 ccatcaaggc accgcctgcc tcgacccggc cggcaacatc ctctggcaaa acaggaaaaa
     4801 cgcctacgaa ccggtgcacg gcaacggctg ctcgccgatt ctggtcgatg gtctgctgat
     4861 tttcagctgc gacggcgcaa cgaatccgta tgtcgttgcc ctccgtgcgg ccaatggcca
     4921 ggaggcctgg cgattcagcc gaccgggcga tccgccgaaa aagttctcct tctgcacggc
     4981 gacgctgatc gaggttgacg gccagaaaca ggtgatctgc cctgccgccg gcgtcgtcaa
     5041 cgccctcgat ccggccagcg gccgcgaaat ctggcgcgtc acgacggagg gctactcggt
     5101 gattcccaag ccgatcttcg ggcaggggct cgtgctgctc agcacgggct acgacgcgcc
     5161 ggaatttgtc gccatccgcc ccacgggccg cggcgacgtc accaactcgc acgtcgcctg
     5221 gacggcccag cgcggcgcac cgcacacgcc gtcgccgctc ttggtcggcg acgaggtcta
     5281 cctggtgtcc gacaagggcg ttgcgacgtg cctggacgtc aaaaccggcc gcgagcactg
     5341 gcagaagcgc gtcgtcggca actattcctc ctcgccggtt tttgccgacg gcaagatcta
     5401 cattcagagc gaagaaggcc ccgccgtcgt gatcgctccc agcaaagagt tcaccaagct
     5461 cgccgacgcc ggctttcagg aacgcacgct cgcctcgtac gccgtcggcg acgattgcct
     5521 gtttattcgg acggagaaga acttgtatcg ggtgcagggc tggtgacaac tcgtttcgac
     5581 attcgtcatt tatgaaccac cgcctcctcg gccgcactgg cgttcgcgtc tcgcccctgt
     5641 gcctgggcac gatgatgttc ggcgcccaga cgaccgaggc cgactcgatc gcgatcatcc
     5701 acaaggccct gggcctgggg gtcaacttca tcgatacggc cgacatgtac gtggccggcg
     5761 aatcggagcg gatcgtcggc aaggccctcg ccggccgccg cgctggcgtc gtgctggcga
     5821 ccaagggccg caacaagatg ggcgaaggcc ccaacgactg gggcacgagc cgcctgcacc
     5881 tgcggcaggc cctcgacgcc agcctccagc gcctcgcgac cgattacatc gacctctact
     5941 acgtccacgc gcccgactac aacacgccga tcgacgagac gctgcggttc ctggacgacg
     6001 cagtacacca gggcaagatc ctctacaccg cctgctcaaa ctttcgcgcg tggcgagtat
     6061 gcgaggcgct ttggaccagc gacaagctcg gactcgcatc gttcgccgcc gtgcagccgc
     6121 tgtataacct ggtcaaccgc gacatcgagg tggaactgct gccgctctgc caggcgcggc
     6181 agatcggcgt ggtcagctac agcccgctgg cccgcggcat cctgaccgcc aagtacaagc
     6241 ccggtgaaaa attccccgaa ggcagccggg cctcgcgcaa cgacaagcgg atgaaggagg
     6301 ccgagctccg cgacgagagc ctgatcatct cgcagaagct ggccgaccac tgccgcagca
     6361 agggcgtggc ggcttcgcag ttcgccctgg cctggtgcct ggccaacccg atcctcagct
     6421 cgatcattct cggcccgcgg acgatggagc agttcgacga caacgtcgcc gccctgaacg
     6481 tgacgatcac ccccgccgac gaagccttcg tcgattcgct cgtcccgccc ggcgagcaca
     6541 gcggcaaagg ctttcaggac cccgcctatc ccgtcctcgg ccggccgaag acaatctaaa
     6601 cgtcaaaagt gcgccgtgcg gcgtcctgat gctgcagcca cctgcgaatc gtggagagcg
     6661 cgtccatgtc aaaacgcctg ctcaacatcg tcgcctgcgt cgtagttggc ctggttgttg
     6721 tcacccttgg ggtcataatc tggcagcaat cgcagcatcc caacgactat tccgaagtca
     6781 ttgactttcc cgctccgccg cctccgaagg tctccgccac caagccggct cctcagccgc
     6841 cccgcggccc gaaccctcca gctccgctcc cgcaagtcaa aaagcagccg gagccagatg
     6901 agccaccgca gcccgccgcc gagccggttc gcaaagggga tctaacctgg tcgcaagtgg
     6961 aagaggcgtt tgagcgcgtg cgccgggcgc gcaaagagaa accggtgctg aatcacgatc
     7021 tcagcagcat gtacgatgac gtggctgcgc cagcctacac ggattccgcc gactatcccg
     7081 cgcattgtgc agctctcgca aaatggcgcg acgagtttcc ccagtctccg acaccgctga
     7141 ttgcgctggc gaagtctcac atcgactacg cctggcaagc ccgcggcggg ggctacgcat
     7201 tcacggtcac ggacgacggg tggcagctct ttcatactcg gatcgacgag gcccgcaggc
     7261 tgttggagaa ggcggccaag ctcgatctga aggacggcga ggcttaccgc cagctcctca
     7321 tcgtggccat ggcggagagc ttgcccgacg agcaggtccg ggcctggttc gatgccggcc
     7381 gcaagatcga tcctggatat gccgcgctgt acacgcaaat ggcgacgtcg atgatgccgc
     7441 ggtggggcgg agcgcccggg gatgtagagc ggttcgctgc cgaagtcgcc gagctcgtgc
     7501 cgggcgagga cggcttggag ctgttcggac tgatcgcccg cgaaattcac cggtacgagt
     7561 gcgacgaccc gacgacgatc ctctggggcg actacgatcg ccggctgctg gccaagtcgg
     7621 ccgacgtgct ggtggcgcgg tacccccagg cgcaaagcgt cgtgcagttc gccgccctct
     7681 gctcccaggt ctgccaggat catgaattgg ctcggcgcat ccgcccgctc gtcggcccgc
     7741 tcaacgccaa cgacagggtc tggatctgga agaacagtta cgaagatttc atcgaatggg
     7801 ccgaggcgaa agagattccc ggaggagagg agtcctcagt ctgggccgca ctggccggct
     7861 gcgtggactt ttcgtttgcc gacgattcgc gctatctctg ggccggccat caattcggcc
     7921 ggcgctcggt cacgctcttc gacttgcaga cgaagaaaat ccgtctggag ctgcctcacc
     7981 ccggtggcgt tgtcaaccac gccctgttcg atagcgcgag caagcagatt gttgtgtccg
     8041 cctggcgagg tccccttgtg ggatggatgc ttttcgatct tggcgattcg gccgagccgc
     8101 tcgtctttac cacgccgcag caggtaacgg ccatcgcgct taatcctcgg caaaagcagt
     8161 tggcctgggt cgaggccaag ggtgggctgc ggctgctcga tctgaccgcg gaagatcgca
     8221 ccccacggga tttgaaggtt ctggtccaca ccttgaaatt ctccggcgat ggaacacgcc
     8281 tggccgcggg cgccgccgag tggtttatct tcgataccgc ctcgggtgag caattgctga
     8341 cgctcccgag tattcgcaaa ccgcagcagg gaatgtggtg tgatcaagtc ctggacatcg
     8401 acgactcggg cagaatctgg gcgacggccc ggtcgctggg aaagccgccg ccgaggcggg
     8461 ctttgattcg ctggtccgca aacggcgagc aacctgagac gataatcgcc gacattggcg
     8521 agggcatggt gaaattgtcg cccgatcgca cgctcttggc cgtggttcgt gactcggagg
     8581 tgaacggggg acccagcagc gtcgatgtgc tcgatgtcgg caagggccaa cgcgtcaagc
     8641 aattggccgg gcactggaat gcgattggcg atctccgctt ctcagcggac agcaagaaac
     8701 tggcgaccat cgcccgcctg tccgacgtca tcaaagtctg gtcgctcgat gaaacggcag
     8761 ccgcggtcaa agagtaatct gccgcttagc cccggtctcc aggccggcgc cttatgggcg
     8821 cggcggctaa gcgagcgtgg cccgttgcac ggtcagcttc tccagccgct gcgggtgcgg
     8881 ctctggcagg cggaggaagg gctgcacgcc gggctggccg gcggcggtgc ggatcagctc
     8941 cagcggcggc tcggcgaggt cttcgcgata gaatcgcgag ctgggaaaga tgtcggcggg
     9001 gtaggtgaag ttcggcagca tggcgagcgc cacgcagtgg ctggcgccga cggcgctctc
     9061 cagcatcccg ccgacccagc acggaatgct ggcccgcgag caaccattgt gaatctgcac
     9121 ggcaaccgtc aggccgccga cacggccagg cttgacgttg acgtagcggc agctcttgag
     9181 ggcgatcgcc tgctcggcct gccgcaggcc ggtgatgctc tcgtccaggc agaccggcgt
     9241 gcggatttgc cgcgccagct ccgcgtgatc gaccaggtcg tcgtgggcca gcggctgctc
     9301 gatcatcgcc aggttcaact catcgacctc gcggaatagc ggcaggtcgg ccagccggta
     9361 gccgctgttg gtgtcgatgt gaaacgtgtg cgacggatgc tcgcggcgga cggcgcggag
     9421 catcggcaga tcccagccgg ggcggaattt cagcttgatc cgcggaaagc cctcggccac
     9481 agcttttccg gcggcgacaa gcaaatcgtc gatcgagtcc atcacgccga agtcggcccc
     9541 gacgggaaca tccgcccggg ccgtgcagcc ggccgcatcg gcgagcagct cgtgcagcgg
     9601 ctttccggcc cgctgcgact ggaggctcca ccaggcggtg tcgagcgccg ccttggcaaa
     9661 ctggttgccc ttgaagtgcg tcagccgctg ctgcagctgc tcgccgctgg cgatctgctg
     9721 ccccacgagg gccggggcga gccattgttt ggccacggca aacacgccgc cggcccattc
     9781 agggctgtag cacggggcgg ccagggacga cgtttcaccc cagccgacgc acgagccgct
     9841 ggccatccgg cagaggacgc tctcgatcgc cgcgtcctcg ccgtaagccg tccgccaggg
     9901 atagatcagc ggcatcgcca catgaaacag gtcaatccgg tcgatctgca tgacttcgct
     9961 cccccgcgtt tcccgaaatg tacaaacttc ggcggcaaaa atggcaggct gtagcgcgag
    10021 aatcccttct aatgacgcaa aggatggaag gggcgaaagc cacaaagtcg agcagagctg
    10081 aaagaaatgc cttgctttgc gcctttgctc cttggcgtct ttgcgttaaa tcgaatcgcg
    10141 ctcatcccca ggcaagtcag ccagcatgca cgatctggtc gagttgcgaa ccgagcggct
    10201 cctcctgcgg cgctggcgcg cggccgatcg cgagccgttt gcggccctga acgccgatcc
    10261 gcgggtgatg gagcattttc ctgcgctgct ttcgcgcgag caaagcgacg agcgagtcga
    10321 gcggatcgaa gcccatttcc agcagcacgg ctactgccag tgggccgtgg agattcccgg
    10381 cgtgacgccc tttgcgggct tcatcggact ttccgttcca cagttcgagg cgcacttcac
    10441 gccctgcgtc gaaatcggct ggcggctggc tgccgactgc tggggccgcg gctacgcgac
    10501 ggaaggcgcg cgggcggcgc tgccctttgg cttcgagaag ctcgcgctgg ccgagattgt
    10561 ttcctttacg gtgcccgcca acgtccgctc gcggcgggtg atggagaagc tcggcatgac
    10621 gcacgatccc gccgacgact tcgaccatcc gctcttgccc gcgggtcatc cgctccgccg
    10681 ccacgtgctg tatcggctcc gcaagaactc cgccggctga agcagaggca aatgtactgg
    10741 cgctcgctaa ttcggtttcg cctgctcacg ctgctgctgt tggtcgcagt gctcggcgct
    10801 ctgctcgtcg ccaacacgcg gcagcactcc caccgtacgc tgggccacgt ctccggcgac
    10861 gctccgccga tctttcccgg tctatggatt tttggccacg actacggctg gccattcccc
    10921 tacaagaccg aatcgttcaa aaccgacgac ttccgtatcg atgtcttcga cgggtttcac
    10981 tggctcggcc tcgtcggcga cagcctcttt ggccttgccc tgctgcttgc tgccggtgcc
    11041 ctcagcgagc tctccctttg ggcgctgcgg cgaattaccg gtttccgccg caaattgaag
    11101 agaaattaac ggcggcggca cctggccggg cgagcgacag tttgtcgcgg cgtcgattgg
    11161 cggagccgtc gatctacgca cgacggccag ctctggcgag ggcatgcgaa atgcagatgg
    11221 cccccgtcac gttgcggtgc gcctcgtcgc cgccaatcgt tccactagcg gaaaaactca
    11281 tcatggatgg gctcaatcgc agtcctggtg gcaaatcccg tcgccggcct ttgctagtcg
    11341 agtcgctaga ggcgcgagtg ctgctggcga cgttgccgag tgggtttgta gagtcgcccg
    11401 tggcagcagg catttccggg gctacggcga tggaactggc gcccaacggc gacttgtggg
    11461 tcctggagca aaccggcgcg gtcaagcgct ttcgccccgg cagtacgacg gccgacatcg
    11521 tgggcagcgt cagcgggctg gggctcaatt cgagcggaga gcggggactc ttggggatcg
    11581 ccttcgatcc gcagtatgcg accaacaagc aggttttcct gtactacacc actacgtcgc
    11641 cgttcttgca caaccgcatc agcgcgttca cggtcgtcga tacgagtgcg gcggactatt
    11701 tctttgccgg cacgagcacg acgccggccg acgacggcag cacgggcgtg ccgacgcaga
    11761 cggtgatttt tgacctggac gcgctcagcg gcgcgaccaa ccacaacggc ggcgcgattc
    11821 acttcgggcc cgacggcaag ctgtatgcgg ccgtcggcga caacgccaat ggaagtaacg
    11881 cccagacact ggccaacctg cacggcaaga tgctgcggat caacaaggac ggcacgatcc
    11941 ccagcgacaa cccgttcttc agctcggcca cgggcgacga gcgggcgatc tgggcgttgg
    12001 gattgcggaa cccgtttacg ttcacgttcc agcccggcac cgggcggatg ttcatcaacg
    12061 acgtcggcga ggtcagctgg gaggagatca acgacggcat cgccggctcg aactatggct
    12121 ggccgcagaa cgagggcaat ccgacggtgg cgccgcccgg cccgggaacg catcgcgggc
    12181 cgatctatgc ctactcgcac ggcggcggca cgtttcaggg tttcgcaatc accggcggcg
    12241 ctttttacaa cccgacgacg ccgcaatttc cggccgaatt tgccggcgac tatttcttcg
    12301 cggacttcgt caacgactgg attaatgtcc gcgacgcggg cggcacagtg actcggttcg
    12361 cctccggcgc acaagggccg gtcgatctgc gcgtggccgg cgacggcagc ctctactacc
    12421 tggctcgcga cggcgggcag gcgttccgcg tgaccttccc gcaagcaccg gccggcaatc
    12481 ccagcgcgct ctaccgcttc aacgagacca gcggcacggt ggcggcggac gcgtcgggca
    12541 acgggcagag cgctcagtat gtcaacgggc cggcgctcgg gcagccgggc gtcgtcgctg
    12601 gcgggacggc tgtgagcctg aacggagtca atcaatacat cgccgtgccc gatgcgccac
    12661 tgggcgccta cggcagcccg ctcagcttcg agacctggtt caacgctccc gccggcgcag
    12721 gcggcacgat cctgggccag acggccgccg gcggcgtgcc cggcggatcg atcccgcccg
    12781 gctacgtgcc cgtcgtgcat ctgggcaccg acggccggct gcggtcgagc ctgttctggc
    12841 atggttcggc cggcgctcgg ttggcctcgc cggcgggcca gtcttacaac gacggcaact
    12901 ggcaccacgt ggcggtgacc tacgtcgccg gcgtcgagac gctgtacatc gacggcgcgc
    12961 aggtcgcgca gcagacgatc agccaggccg gctacgcaac cgactacgat tacttcatcg
    13021 gcgtgggcca gacgagcctc tgggaagcgg gcaatggcgg ctggcacttc ttccgcggcc
    13081 ggctggacga agcggtcttc tttcagcggg cgctgacgcc gacggaagtc gcccagcatt
    13141 tcgcccaggg caatccaccg ccggtcaaca gcgctccgat cgccacctac cgcttcgacg
    13201 aggcgagcgg gatcacggcg atcgacgcca gcggcaacgg caacagcggc atgtacgtca
    13261 acgggccggc gctgggccag cccggcgctc tgccggccgg cacggcggtg agtctcaacg
    13321 ggaccaatca gtacgtcgct ctgccggatg cgcccttcgg cacgtacggc gggccgctga
    13381 gcttcgaaac gtggttcaac gccccggccg gcgcgagcgg cacaatcctg agccagctgg
    13441 ggggcggcac cacggtcggc ggcgcggcca cccgcgggta tgtgccggtc gtgcacctcg
    13501 gcgtcgatgg ccggctccgc tcgagcctgt tctggcacgg cagcgcgacc cagcgcctga
    13561 cctcacccgt gggcgccgta ttcaacgacg gcaactggca tcacgtcgcc gtgacctatg
    13621 ctgcgggcgt cgaaacgctg tacatcgacg gtgtgcaggt cggccagcag accatcaacc
    13681 aggtggccta tgcggccgac tacgactatt acctgggcgt cggattcacc gacctgtggg
    13741 ccgccggcaa tggcggctgg cactacttcc gcgggcggct cgatgaagcg tcgctctatc
    13801 agcgggccct gacggccagc gaggtggccc agcacttcgc ggcgcgggcg taggttacgg
    13861 aacagccgcc cgccgcggct cggaactggc ggcggcggtc ggagcaccgc gcaagatgac
    13921 cgcggccttg aggagcgctt cgtacttggc gaccatatca ggcccggaga gcttgggcga
    13981 cagggtcagc gtcgatttga gcatctgctc ggcctggagc gccttttcct tgttgttcgt
    14041 ggcgcgggcc tggcgcacga ggcagcgggc ggcgttgtaa agcacttcat agtattcggg
    14101 caggcgcgtc ttcgctcggc caagctgcac ccgcagctgc gtccagtggg cgatgcactc
    14161 gtcgtacatg gccggcttgg ctgcggccag gctctccagg atgtagccct tttccagctg
    14221 aggttcgagg gcagtcggat gggctttgat cagggcatcg acttgcttgt tggcttcgac
    14281 gagcttgcct tcgctgcgca acaggccgat gatccgcgag cgaatccgcg tggtggccgc
    14341 gccggcggtg gccttggccg attcgtcctt gtcgatcgtg tcgagcagtc gctgatagac
    14401 ggtgcgggcc tgctcgttct tcccgagcag cacggacgtg tcgccgacgt agatcagctg
    14461 cggcacggag agcaggttgc ggccgtagat ggcgtcgatc tgcttcccga ggctgtctcg
    14521 cagtgcggcg acgtgcggat cggcgggcgg cgcggatggt ggcggattgc cgatagcggg
    14581 aggaggcgcg gtgttcatag gcgtcccact gacgcccgtc tccgactcgg cctccagctt
    14641 ccgcagctcg atggcgaaca gcttcgacaa atcgacgagc aggctgttgg gctgcgcgga
    14701 gtcgccgctg atgcttgcga acgccgtggc ggcttcgccg gcctgcgcgg catcgccgac
    14761 ggccagcagc gcacgcaccg ccccgacgta ggcccgctgg gtcttctggt cgattgctcc
    14821 gggcggcttc tcggccaggg ccttcatcag cggggcgaac agctcggcgg agtctttgaa
    14881 ttgctggccc tcgagcatga tctcggccag tagcagctgc gtatcgaaga gcggctccgg
    14941 catgggctcg gtgccggcct gccacgcggc ccgctgccgc tgcacgctag tttgcagcgc
    15001 ggagacggcc tcggcccggt gcttcttgcc ttcttcggcc cgcgccggat cttcggattt
    15061 tttgatgtcg acgtactgcc gccagcgaat ctgccccaga atctgcagcg ccgtgggata
    15121 tcgcttcgac tgcggcgtca ccgccgcgac ctgctgctct acgccggcaa agtcgccctg
    15181 gatcaggcgg gcctgggcca gagccatccg ggcgtcgtcg ccgacgggat tgtccggcca
    15241 gcgggagatg gcataggtgg ccactttttc cagccgcgcc aaagccgccg ccttcccgtc
    15301 cgcgacaacc gtcgaataca cctgcagcgc ggcggccaca gcgaccgccg aggtgctctc
    15361 ggcaatcggc tgatctgaat tgtcgcggac gatctcgccg gccagcgcca ggcactggtc
    15421 catttcgccc gcggaatagt gcttcagcac gcgctggtac agcacttgag cgagcttacc
    15481 cttcacagcc gtgacttgct tgttgtcgcg ggcggtcgtg gcctgcgtga tcgcttcgcg
    15541 gtacatggtc tcggccgagg accagttgcc gctggcggcc gcctcgtcgc cgacggcgat
    15601 ccgctcggcc agcgtcatgt tggcggaagc ggtcgtattc tcgacgatgc cccgccgcat
    15661 catcgtcgct tcgtggcggt actccgaatc gaccttgccg atcgccgtca gggcggcaat
    15721 cgcatcgcgg gtgagcttct gcttctcggt cgccgtgcgg gccgattcga ccagcttgag
    15781 ccgggcccga gtcgtttcca ggtgcatgcc ctggtagggg cccgtgctct gccagcgctt
    15841 attcgtcagc aaccactctt cgccctcggc cagcaccttt ttcggctcgg cggtcttggc
    15901 gatcagcttc aggcgaaaca gggccgcctg gccgaagagc ggagcgagat ccagatcggc
    15961 gtccttgcct tccggcgcga ggacgaggac ctcgtcgtag acgtcgaggg ccgtcgtcgt
    16021 attgccttgc tcttcgtaga ccaggccgtg ccacaagtgg gccagcgacc ccagcggctt
    16081 gccccggtat tcctgaaaca gcttgtcgag ggcgtcgccg gcctccttga gcatttggac
    16141 gcgttctttg tctttggcgt tttcgatcgc ctgtgcggcg aacagaacgc actgggcccc
    16201 gtagaaccgg gcctcaagcc acgcccaatc gagaagctcg cgcggattgt tgcggccgcg
    16261 caccgggcgg tcggcgggca gcgcatcgag ccgggccttg agggtcttct cggcatcggc
    16321 aaacagcttg cgggcttcgg tgagcgtcgt gcgggccgcg gtgcgatgct ctttctgctc
    16381 gtcggcgctg cgggcggccc gcatcttcgc caagcttatc tggccgcgga actgggcggc
    16441 tttgccctcg tccaggagcg tcgaagccgc ctccgcgtgg tctggatgat ccttgaagaa
    16501 ttgctcggag agcttcttcg cttcggccag gcggctgtcg cgctgcggcg cgtcgtaggc
    16561 ttcggtcgcg gcgatcttca gcgacttgct gcgctccagg acgaggtcgc gcttgaggtc
    16621 cgcgggcaag tccggctggt cggcgatctg gtcaaggtac gccagggcct gctcaccata
    16681 gcccttcagc tgcaaggccc ggaggaattc gagatacggc tcgccagccc gcccgccgcg
    16741 cgcaatcaac aagccgcaag ccagaacaac cagccacgat ctttggacca aaacggaatt
    16801 catcatccgg ctgggctact gggccttgtt aaggtccttc ttgacgtcgt cgatcttgta
    16861 gagcggcaga tagcggtaat agacctcgag cgtcagacag ctcaagctgg tgaccatcag
    16921 gcggccgccc tggttgcccc acggatcggg ctcgggcttg tccgggtccc aactgccggc
    16981 ctggcacccg gacttctctt gcgtgtcgat caagatccgc cgcatggtgc ggttccagtc
    17041 gtcccattgc gctccgggca cgttgtgcat cacctgcgtc gcgtagtacc agtaataggt
    17101 gttccgctga ttgaccgacg gcttgcgctt cgagaggtat tccacgccgc cgatgatgac
    17161 cggatcgtcg cggcgagcgc cgaggtattg cgaggtgagc aggccgaccg acgtcatcgt
    17221 gaacgtcggg ccgccgccgg gcgtgtagct gaattcactc ttgtactggc caagcgagac
    17281 cttgctcagg tattgcttcg cgccttgcag ggaggcggga ttgacgttga gcccggccat
    17341 ctggccgctc ttgagggcca tcacctgcca gccgtagacc gacgtatcgg actgctcggt
    17401 gccgtgctga tagcgccagg cgccgtcgcg gttctggccc gactggatga agtcgatggc
    17461 ggcctgggcg gcgtagccga tccgcgaatc ctgggtcatg ccataggctt cgcagagagc
    17521 gatggtcgcc aggccgtggg cgtacatctg cgagccgttg accgaaaggt cgccggtctt
    17581 cttctgattc ttgatgagga agttgatgcc cgccaggatg ttcttttgat acgggccctt
    17641 gctctcgtgc gtttgccccg cggccaggaa cggcagcagg ccgaggcccg tccccgcgcc
    17701 gtcggaattc gctccacctg ggccggagca aaaaccgctg cggcacatgg gcgtgtgatt
    17761 gagactccat ttgccgtcag ggctctggtg ccgggcgatc caattcaagg ccgccgcgac
    17821 cgcccgctcc gactgcttcg tgccgccggt tcccagcatc gcttcacgca tgcccgtgcc
    17881 gcggctgccg aatcccgtgc ctgcgccgcc ggtgcccatg ttcttgccga agcccgcgcc
    17941 ggcatcgacg ccgccgccgc ccttgacgat cggacccagg ccggatgcgg cgacgttgaa
    18001 cccgcccagc ccgccgaagt tcgagtcgcc gccgacgatg ccgccgcccg cttcggtgaa
    18061 gatcggcgag ttgtcgttga actgggcctc gaccgagggg gcctcggtca ttgtcagcgt
    18121 ctcggtcgtc agctccgtcg ggtcggtcgg cgtgtagccc acctcgaaat gcgtaatctc
    18181 tggttccagc gactgcggct cttcgatcac ctcgaacacg ggggccgcgc cgatcgtggc
    18241 cgcgacgtga atcgtgccga gcaccagtgc caggatgatg aacagcgtca tgtggatggc
    18301 catactcgcc agccaggact gcgtggcgat gccgaccatc aggtccttca tccagccttc
    18361 cgagccggac gcgacttctt cagggtgctc ggctgcgctt gcttggctgg cctgctcggc
    18421 gggagcagcg gcgggcgctg gcttgttcgt cttgatcatg gaagcagctc ctgggctgtc
    18481 agattctaat tgttttggta gtcggcgttg cgaatgccgc gagcggcggc ctcattctcc
    18541 tcttcgaccg aatccagcca gtcgcgcaca gcgccagcct tgtgcggcgg aatcttgccg
    18601 tcgtcggcaa actccgcctt gatcgggcgg ctgcggcgat agagccacaa agccagccgg
    18661 ccgatgcaga acaccgccac ggtgccgacc acccaccaca cccagggcga gtttttggcc
    18721 ttccagagaa actcccagtc cgactgggcg gcggcgacct gggctggacg ccattggagc
    18781 cggccgatca atagcggcgc gggcagcggc ttatccgtgg ggccggcccc ggcgtgctga
    18841 tagccctgga ccttcaggaa atagccgacg aaggtcgccc gctcgtggat cctggccccc
    18901 tcgggcatgc ccggcggcag ctcgtcggtc agcagcatat agaaccaggc ctgcgattga
    18961 tcggtcgtcc cgagaatctc atagactttt tcgacgccgg ccgagttctc cgccgccggg
    19021 tacgacagaa tccgccgaac cgtcaggtcc agccgaaaca gcttcccgcg ctgcgcgtcg
    19081 cgcgtgttgc ggtcgataaa ctggttgaag agcacgttct tgtcggcccg ctgctcgagc
    19141 tgggccagcg gctggttctt ggaccagcgg aagagccgcc aatatgccgg catctcctcg
    19201 cggcccatgt cgagcgtgaa gtcgctcagc gcctggaatt gctcttgtgc ggtggctcgt
    19261 tcatcggggt ccagatcggt tccgtccact acctgctctg gcgcagctgc agcggctgcg
    19321 ggacttaccg cagcaggcgg cttggaatca gcttccttgg ttgcagttcc tttcgccgca
    19381 gttacttcag ccgccgtttc ccccgtcaac cagacccagg tgccaggatc ggccgcgcgc
    19441 gcgatcagca tcgccagcac cgccagcatc gcgagcatgc tgatcaggcg cagcgctccc
    19501 gcgggcctgg ccgcggccgc acgcactcgg tcgatcgttc gaatcgcccc gctcctttcg
    19561 ctcgggaact agcccgccgg caactcgaca aagctgagct tgctcagcac ttcgccattg
    19621 ggcagctttt gccgggtgca gacatcgatg atctgcatca gttcatcata ccgcaagcgg
    19681 gaatccactt gcacgatcac ctggtcaaac ccgctgcccg gatcgcccag aaccgtggac
    19741 agtttgcgtt ccaaagcggg cacgctgccc atcgtcgcgt cgcccacggc catctgccgc
    19801 aagccgccgt ccgggttggc aatggccgtg atgaccagcg tattcagtcc ctgcacggga
    19861 ttcgtgttgc tggcgtcgga gcccgccttc tgaccggcgt tgacgttggt tatcggctgc
    19921 ggcggcggca tccgcagcag gacctgccct tcgaccggag ccggcttgaa ggtcaggatg
    19981 aaaaaggtca gcagctggaa cgccatgtcc agcatggcag ccagattcag ctcgacctcg
    20041 ccttgtgacc ggcgtttttt acgacgacgc acgtctacgt ctcctcagtc ttgttcatcg
    20101 ccttgagggc gaacttgcgg tagccgtgct cctggcattt gctgatgacg cggttgagca
    20161 tggcgaacgc ggtgctcttg tcggcgcgga tgaccacggt gctgggcagt tcgtcgccgg
    20221 ctttgagatt cggattcgtt tggcgggcca ccatcagact ggccgtggcc tcgttggcaa
    20281 tgtaagtcga aatatcggga atgagctgcc catagacgcg gagctggccc tgcgagttga
    20341 tgttgaggat cagcaggtcc tcctggccct gggtatcgac gggccgggcc gagccgatca
    20401 ccggcaactt caggtccagg tccatcgacg cggccttgaa gttgatcacg agcatgaaga
    20461 acgtgatcaa ctggaagacc atgtccagga tcggcgtcag gttgggctcg gcgctggagc
    20521 tatgcgaaag ggaagcagac atgacggttt tcgaaggtat ctccgcgtgt gcggcgccgg
    20581 ccgccgcatt taccgcgggc gatcgtggtc aggttatccg cgaacgggat gggtctgcgc
    20641 agcgggcgcc gcgccgggct tcttggcttg ttgcacgagg cggcggacga gctcgtcggc
    20701 ggccagcagc gtctcgacgg aaattttggc gacgcgattc ttgaagaccg cgaagaagta
    20761 gatcgccggc acgctcaagg ccacgccctc gaacgtgagt agcagggcct cggagatgcc
    20821 gccggccacc tcactggcct tcagctgcgt atcggacaag gcgatgacgg caaagctggc
    20881 gatcatgccc ttcagcgtgc cgaccagccc gatcatcggc cccagcgtgc ccagcacggc
    20941 cagcatgctg atcttccgct ccatatccac cgtttcggcg tcgcagttgc ggtccatcac
    21001 ttcgcgggcc tcggccaggc cgctgggcaa ttccgcgatg cccacgctga ccagcttgcc
    21061 gtacagcgac gagctgtggt tggctgcgtc gtacagcccc tggtagtcgc ggttggcgag
    21121 cagctgctcg cactgggcca cgacctccgt cggcatcgcc acgtccagcc gcaattcgat
    21181 gaacaagcga atgaccgtgg cgacgaaata gatcgacagg atcaggatga acaagccaat
    21241 gagcccggac gacatgatga accagacgaa ccggctcttg ggcttgggcg ctggggcggc
    21301 tggtgccccc gcgggagccg ccggggctgc ggcaggttcc tgggcggcgg ccctcggcag
    21361 gtcagcctga gtgatggtga agaccgccga gaacatcaac agcgcaacaa tccatcggtt
    21421 ccaaacggcc atcattactt ctcccggcgg tcaggcgagc gcgcaccctg gacgcacggc
    21481 tgacccgagt ttgagtttct tgcgattcga cgcctgattc gagcgtgctc acgcagaata
    21541 ttcccgtcaa gactgcaggc ggatatccct gcggaaaagg cgattttaag caatttctgc
    21601 ataaagttct aggcgctggg aaccactaca tcttcggcac cggaaccagc ggcgtgctgc
    21661 ccgtataggg attcggtgcg tcgtagtcgg ggatcaactc gacgccgaag ccacgattca
    21721 gctcgtactg agcccggccc gcccaagggg tgccggggtg gtcaaccagg atcttcttga
    21781 acatcgcggt ggcccgctcg atgtagggct gcaccttggc gcctgtaatg atttcatggc
    21841 gagtgcggat gtcccacgtg acgtgcgtca ggttcggcgg acgagtgagc ggggccgtct
    21901 tgggattttt gatgaactcc tccaagtagg cgccgtattc gtacatgcgg gcctggtacg
    21961 cgatgatctg cgcgtgcagc aggtcgtaat tggcctgcca ccgcggcaag gcctcgtggc
    22021 tgcgcatgta ctggagcttc tcgacctcct gctgggcctt ttcgaggtac tggatgtaca
    22081 cgatcgcctt ggcctgctcc tgacgggcct gacgaacgaa agcctgcggg tcgcgggcga
    22141 attccacccg catctcgatg atcttctgag cgacgggatt gtaggggttc aggtcgtaaa
    22201 tgaccttctg gagcatcgtc cgcagtggcg aattgtcgat gtcgtgcttc acttcctgcc
    22261 gcgagcggac gtcgggcaag tagggctgca gcgcttccaa gtcgtaattg cccttctggc
    22321 cgcggacgag gttcgtctcc aggctgggca gcatgaagaa gatgccgccc gtttcttgcg
    22381 acatccgcgt gcattcgtac ggtccgaagc cggagggatg agcgtcgtag cggcggtgga
    22441 agccgtcagt ctgcaactgc tcgacgaagg ccgtctccgg cccgcggtcg atgcggatcc
    22501 agtgcgtgtt ccgggtctgc ggatggaccc agcgaatatg cgcgtaggga tatccgaata
    22561 ccgcctcgcg cccgagcacg taaatcttgc agcgggcggc cttggcctcg gcgatgacct
    22621 gctccaggtg ctggtcgttg tcgctccgct cgcccgattc gtcggtcacc acgatgagcg
    22681 ccatctgccg ccgcgaacgc tgggcgtact gcttgtggat cgccacggtt tgcaaaatcg
    22741 ccgagcacat catttcgatg ccgctcttat cgactggcac ttccgcgatg gcggcccgta
    22801 tttcgtcata gtcgtgcgtc ggctggcgag tgtggaccag gaacttctcg ccgtagctgg
    22861 tcacggccgt ttctaacgcg ccgctgttat tcgaccggag cagccccagc tgccggtaga
    22921 cgtgctcgat ccgctcgcgg atttccttct ggtcgtcctg catgctttcg gactggtcga
    22981 aggcccacat cgtcagcacg gggcccttgt cgagcatcca caggatttcc tgtgtcaggc
    23041 ggtcgagggc ctgctgatag ccgccgacga tggcccgcga gtcgccgatc tgcccgtcgg
    23101 gaactgcgtc gatcagcttc cgccctcccg gcaggttcag ctcgattccg ccgatgccgc
    23161 cggtgcccga gccgacgccc gagctgaaga gcgagccgtc gagctgcagg ccactgccgc
    23221 cgcggccgct ccccttgcca ccgatcatgc ctcctgaagg gccgccactg ccgccgcctc
    23281 caccaccggt tccaccaccg ccgccggcca cgtccaccgc cgtcgcggcg gccgacgcaa
    23341 tcgacgcgct cgaataaagc gcctccggcc gcgtgtaggc cagcgtcagc gtcggctcca
    23401 gttctgcttt gagcagctct tcctgccggc tccactcttc cacatcgaag gcgatcggcg
    23461 gcggcttggg catcgtgatc gtgcccagca ccaacgccag caccagaaag accgccatat
    23521 gcacgccggc cgatgcgagc gtgcacgtca ggctccgcat ccattcgcgg cgggcctcgg
    23581 cggcatccag atcgtcgtcg ccggccaatt cgcccgcagc cggcacgccc acaggcgatt
    23641 ctccagcctc tcccgcaggt tccccgtcgg gaatctcctc gtaatccgta agttcttcga
    23701 cgtcggtcgg ctgccggcgg ctgactggca cgccggacgg ggcaacggaa ctggccatag
    23761 acaaaatccc ccggccgcag gatccgcgag tccctgtagc ttgcccatca tacccggaaa
    23821 gcagctgccg atgaatccac tattctaatc gagcaaactc cgggaaagta cttcacaaaa
    23881 ccgcgttatt tcgatccgcc gcttagcccg gcccccaggc ccggttgggc cagtagcggc
    23941 cagctggcga aggctacttt cgcttcggtg tgagggtggg gtcggctttg accttggcca
    24001 gcacggcgag agccgtctcg acgttcttca ccaagtcggc cggcgcttgg ggcgaggcct
    24061 tcagccgctg gtggcagtcg agcagcagcg gctcggcttg ggcgaggtcc ttgcgcagca
    24121 ggtagatgcc ggccaggcga tagagcgact ggagtgtgtc ggcgttattc ggccccagcc
    24181 gcaactggcg gccctcgaac gcgcggcgga acaatcgctc ggcgtcctgg ctgcggtcgg
    24241 cggcgtgata gagcgtaccc aggttgtgga caacgatcag cgtcgtctgg tcgtcggcgc
    24301 cgagcttctc gtcgcagatg gccagcgttt cctcgcacag gtcaatcgcg tcgccgacgc
    24361 ggttcaggtt catcagcgcg ccgcccaggt tgttgcgggc ggtgagcgta ttggcgctgg
    24421 ccgggccgta gcgctcggtc agcgactgca cgacctcttg atagagcggc agggcttttt
    24481 cgggctggcc cgcctctttg taggccagcc ccaagttgtt gagggcccag acgatcttgg
    24541 gatgattcgg cgggagtttc tttcgcagtc ccgcgagcga ttcctcggtc agccggactg
    24601 cgtccccttc gcggccgctg agccggaagg ccgaggccag gttgctttgt gcctggagcg
    24661 tgctggggtg ttcgaggccc agcgtttcct tccgcaagcg gtaggtctct tccgcgatcg
    24721 acagcgcgtc ggctacgcgg ccggcggatt cgtagatctc ggccagcgcg gcagcgattt
    24781 cgagcgtgtc cgggtgagcc ggtccaatct tcgccgccgc cgtttgatgc gcggccgagg
    24841 ccatatcgat cgcgtcctga aagcggcctt cctcggccag catctgggcg atgcccgttt
    24901 tcgcggtcag cgtccggggg tgctgcggcc ctagccgcgc ggaaatctgc tccaggttgt
    24961 cttcgaattg cttccgggca tcggcccggc ggccctgctt gcggtagatc gaagccaggt
    25021 tcgccagcga accgagcgtc aggggatcgt gcgggccgta cagcttcgtc cgggcagcca
    25081 caacctgctc cagaatgggc agggcctggc gccactggcc ggcctgcgag aggagcgtgc
    25141 cccgttgatg ctggactttg agcgtcgatt cgtgctccgt gccaaagacg gccagactcc
    25201 gctggacgag gtgctcgctg cggcggaggg cttcggccgg ctggccgtcg gcctggtaat
    25261 cctggactag gctaaactcg acggccagcg cagactggga atcggccccc ttcacgcgct
    25321 ggcagcgagc aagcagctct tcgtgcagct gaatcgccgc accagtcttt ccggtctgcc
    25381 gcagcgcgga gccgaggcgg tcctcggctt gcatcgtggc cgagtggttc ggccccagga
    25441 cctgccggcg gacgcgcact gcctcctgaa aatgcgcaag ggccttggc
//
LOCUS       DASRJB010000182         8782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1463062, whole genome shotgun sequence.
ACCESSION   DASRJB010000182 DASRJB010000000
VERSION     DASRJB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463062"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFB80_05095"
     CDS             complement(<1..251)
                     /locus_tag="VFB80_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33169.1"
                     /translation="MLIYRAGIVLALVALALPARAQDAAAAKELQDTIDKYVAAYNAG
                     SLDQVMSHWAENADFVDIRGRFHEGRDLVSALFRRGFAN"
     gene            412..624
                     /locus_tag="VFB80_05100"
     CDS             412..624
                     /locus_tag="VFB80_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33170.1"
                     /translation="MQITSQQLQAIDNGEPVPLVVEGRPCILVPGTIDQLRDALDDWD
                     PRTMQRHMARLMADDWNDPAMSAYDE"
     gene            617..973
                     /locus_tag="VFB80_05105"
     CDS             617..973
                     /locus_tag="VFB80_05105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZN33171.1"
                     /translation="MSSLTVTKGDVVLVDVPYLDATQSVRRPALVVSDSSQMLDVIIA
                     GITSRIRAPLPPTHYVVDRKHPDWSASGLRLVSAIRGDRLFTVETASIQRVLGRLSPA
                     TMLAVDQCLKRALRIA"
     gene            1054..1692
                     /locus_tag="VFB80_05110"
     CDS             1054..1692
                     /locus_tag="VFB80_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33172.1"
                     /translation="MPRRFLPALALALLPLAALRAADDGFVTIFDGKSLDGWKKTTEN
                     PDSIQLKEGAIVAHGSRSHLFYVGDEKPFKNFHFQCEVMTKANSNGGIFFHTKYQETN
                     WPAQGHECQVNNTFKADPQKTGGVYHVAKVLEAPAKDDEWFKYEIIVEGQHVIVKIND
                     KVTTDYTEDPEKLAKEKGIEPGRRVGEGTFALQAHDPGSTVLYRNIRVKRLP"
     gene            1822..2901
                     /locus_tag="VFB80_05115"
     CDS             1822..2901
                     /locus_tag="VFB80_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZN33173.1"
                     /translation="MRIALLASALLAGLAPFIGAARADELSDAKTALEKAGVRVLATG
                     VMLANEGDLGKELGKSAQLRRTMTLVNKDLQAAEQTAAKGQQALNQLKLQHVQLSTQL
                     AKINPNDVALNNKLVGALKALEGQFELGAQEKAKLDEQVKAARIKASEAREAYLEFVL
                     AARKLGDKLEADYAKKVNDPEVKAALARLNAAAGKQFTLAPTAALIASLKALKRLEDT
                     ILSEAIELRSDGRSLWVKVKVDEKYDQEMVVDSGASLVCLPHAVAAKFGMKPTEKDPL
                     VTLVLADGREIQGRRMNIKSLRVGRFEVEDVDGVVLGEDAVAAQPALGLSFLEHFKFE
                     IDTGAKSLTMVKISADGAAAKAANK"
     gene            2929..3807
                     /locus_tag="VFB80_05120"
     CDS             2929..3807
                     /locus_tag="VFB80_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN33174.1"
                     /translation="MPVTRRQFLATSAAAGTMAALNFGPRPAAAAEEPAIAFGLVTYM
                     WGADWDLPTLLANCKKTNVLGVELRTTHAHKVEPALNDKQRAEVAARFSDAGVTCVGI
                     GSDERFDNPDPAVVKKAIEATKEFVRLSHDIGGSGVKVKPNDFHPNVPKEKTIEQIGK
                     ALNELGEYASGFGQQIRLEVHGKCAELPTIKAIMDVATNDNVAICWNSNKTDLAGGGL
                     EANFNLVRKRWGATCHIHELESADYPHQQLINLLVKTKWEGWLLMENASKPADRVAAM
                     AQQRKLFDEMLKKAAG"
     gene            3979..4800
                     /locus_tag="VFB80_05125"
     CDS             3979..4800
                     /locus_tag="VFB80_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN33175.1"
                     /translation="MIEVRQLTKSYADLRRGQFVALAGISFDAHAGQVYGLLGPNGAG
                     KTSALRILSTVLQPTSGTAKVNGCDVLTQPALVRRQLGFVSTNTAVYDRMTAWEMVEY
                     FGRLNGLAEEPLRERMEALFTRLQMNDIRDLLGAKMSTGMKQKVSIARALVHDPPLLI
                     FDEATAGLDVLVARRLLLTVAQLRDQGKCILFSTHIMREAERLCDRLAILHRGHILAE
                     GTLSELRERYHEQDLEELFFRLISQHDGEKEGLRDGGTANTAAPSLPLSLSPSRR"
     gene            4847..7117
                     /locus_tag="VFB80_05130"
     CDS             4847..7117
                     /locus_tag="VFB80_05130"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HZN33176.1"
                     /translation="MNWSNVKLIYLRELRDQLRDRRTLFTIAVLPLLLYPLMGMALLQ
                     VQQFRKEHASKVRIIGAESLPAEPQLIVGQQFAPHLNQRLLELELTPAAGRSPTEVQA
                     EVQRELREGAFDAAVYFPPDFARRLQDFRRTLPAATGAGGDAGKVPQPQIFFNTASDK
                     SKVATGRVEAVLHQWREAMVQQNLKASGLSPATTQPFDVASTDVAEPASRRAALWSKI
                     LPFIVMIWALTGAFYPAIDLCAGEKERGTLETLLSSPAARGEIVWGKLLTVMTFSMLT
                     ALLNLAAVTAMATFTISRLQKMGGVNLPFEVGPPPWTALVWLVLALIPISALFSALSL
                     AIAAFARSSKEGQYYLMPMLMISLPLMMLPMLPAIELDLGYALIPLSGVLLLLRALIE
                     GQYLEALRYSVPVVGVTAVCCLLAIRWAIDQFNNEAVLFRESERFGLGLWIRHLVRDR
                     MDTPTVGEALLCGVLILIVRFFAGLTMAEPTNWRSFVTSTLTIQLAFVAAPACLMAIM
                     LTRKPLLALSLARPSFLRTIPAAGLLALLLHPVLYWLAHGIELVYPQSPGVAERLAEM
                     LQFVDHAPLWQALLLIALAPAICEELAFRGFILSGLRHLGHKWGAIVLASVFFGLTHF
                     MLQQSISACVIGIVIGYVAVKTGSIFPGMLYHFVHNGSSLLLSRVTPDLLESYPLLKL
                     VFTPGAEPGSYFYRVPFTVAAALLGLGLLWWFQRLPYHRSAEERLQEALDHQEVGGQE
                     PAARSQGPALLTGHGQ"
     gene            7275..7712
                     /locus_tag="VFB80_05135"
     CDS             7275..7712
                     /locus_tag="VFB80_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33177.1"
                     /translation="MSRALVALSLILVLCAAVSGCGKAAATSGEPEAKERLKCLLELY
                     RHYVLANGKGPANEQELRAYGQKLTPQDRTAFMIGDDLENIFVSPRDNQKFQVRYGLK
                     LEPTGATRAVAWEATPQNGKRYVALTMLYVEEYDEETFKSYNH"
     gene            7740..>8782
                     /locus_tag="VFB80_05140"
     CDS             7740..>8782
                     /locus_tag="VFB80_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33178.1"
                     /translation="MFSSVRSRRGFTLVELLVVIAIIGVLVALLLPAVQSAREASRRT
                     KCMNNLKQLGLAMHNFHDVYLRFPSSGWYDWCRAMPNAVPTGMSAGDWGQNGCIVAYG
                     NVNSFSNGPVVANQPTGQPWPAPPQQAAGWPFQLLPYVEQNAAQNLGGGHIRNTGLPA
                     YVCPSRRPPSQRLTLGSALGAKPNCYATPYFGPVSRDANTIRNTEASFFGIIVPSEPP
                     AANTGRGDHVVRMASITDGTSNTLLLGEKWLRPDQYLTGAWMDDHNFASAHDQDHLRI
                     ADEPPVKDTNNNPLTGVRVAQGDNNPCCDYWRDAFTRNPSPRLGSYFGGPHPGGMNCV
                     LADGSVRGINWTIT"
BASE COUNT         1590 a         2920 c         2807 g         1465 t
ORIGIN      
        1 ttgttcgcga atccgcggcg aaagagggcg ctcaccaggt cgcgcccctc gtggaaccgc
       61 ccgcggatat cgacgaagtc cgcgttttcc gcccagtggc tcattacctg gtccagagag
      121 ccggcgttgt aggccgcgac gtacttgtcg atggtgtcct gcaactcctt ggccgccgcg
      181 gcgtcctggg cccgcgccgg gagtgcgagc gccacgagtg caagaacaat tccagcccga
      241 tagatcaaca tggcgaaggc ctccacaggg cgagaagaaa ggccggcgca ttatcgagcg
      301 cgctccccgc gcggcacaag caaccgatca tcgtgagtgt atgcgatggc cgggccgtaa
      361 aaagttgctc cacgggcagc gcgggcgtag gctgacaata aggagaccgc gatgcaaatt
      421 accagccagc aattgcaggc aatcgacaat ggggaacccg tcccgctggt cgtagaaggc
      481 cgcccgtgca ttctcgtgcc tggcacgatt gatcaattgc gcgacgcgct tgacgactgg
      541 gatccgcgca cgatgcagcg ccatatggcg cgactgatgg cggacgattg gaacgatccg
      601 gcgatgagcg cctacgatga gtagcctcac cgttacgaag ggcgacgtcg tgctcgtgga
      661 cgtgccctat ttggatgcaa ctcagagtgt tcgtcggccg gcgctcgtgg tttccgattc
      721 gagccaaatg ctcgatgtga tcatcgccgg catcaccagc cgcatccggg cgccactccc
      781 gcccacacac tacgtcgtcg atcgaaagca tccagattgg agcgcctccg gcctgcggtt
      841 ggtatcggca attcgcggcg atcgcttgtt tactgttgag acagccagca ttcagcgcgt
      901 cttgggtcgc ctctcaccag ccacgatgct ggctgttgac cagtgtttaa aacgggcctt
      961 gcgaattgct tgagccggcg gacccggatt tacagcggcc agcccgccga tcacactggc
     1021 gagctaatcg acaatcctca cggagactct ctgatgccgc gccgattcct gcccgcattg
     1081 gcactcgccc tcctgccgct cgccgcgctt cgcgcggccg acgacggctt cgtcaccatc
     1141 ttcgacggca agtcgctcga cggctggaaa aagaccacgg agaaccccga cagcattcag
     1201 ctcaaggagg gcgcgatcgt ggcgcacggc agccgcagcc acctgttcta tgtcggcgac
     1261 gagaagccgt tcaagaattt ccacttccag tgcgaagtca tgaccaaggc caacagcaac
     1321 ggcggcatct tcttccacac caagtaccag gagacgaact ggccggccca gggacacgag
     1381 tgccaggtca acaacacctt caaagccgat ccgcaaaaga ccggcggcgt ttaccatgtc
     1441 gccaaggtgc ttgaggctcc ggccaaagac gacgagtggt tcaagtacga gatcatcgtg
     1501 gaaggccagc acgtgatcgt gaaaatcaac gacaaagtga ccaccgacta caccgaagac
     1561 cccgagaagc ttgccaagga aaaggggatt gagcccggac gccgcgtcgg cgaaggcact
     1621 ttcgccctcc aggctcacga tccgggcagc accgtcctgt accgcaatat ccgcgttaag
     1681 cggctgccgt agcggcccgc agccaaccgc gctggattgc ggtcccgcgt tgggctaaac
     1741 tcgccaatgt cgaaatcgcc cggcttgccc ggcgcgtttt ggtttcgcct gcctaagcaa
     1801 tccttcctgc taggatcacg catgcgcatc gcacttctcg cctcggcgct gttggctggg
     1861 ttggccccgt tcattggcgc tgcgcgggct gacgagctga gcgacgccaa gacggccttg
     1921 gaaaaggccg gcgtgcgggt gctggcgacg ggcgtcatgc tggccaatga aggggacctg
     1981 ggaaaagagc tgggcaagtc ggcgcagctc cgccgcacga tgactctggt gaacaaagat
     2041 ttgcaggcgg ccgagcaaac cgccgccaag ggacagcagg ccctcaacca gctcaagctg
     2101 cagcacgtgc agctcagcac gcagctggcg aaaatcaacc ccaacgacgt cgccctgaac
     2161 aacaagctgg tcggtgcgct caaggcgctg gaggggcagt tcgagctggg cgcccaggaa
     2221 aaagccaagc tcgacgagca ggtgaaggcc gcccgcatca aggccagcga ggcccgcgag
     2281 gcgtacctgg agttcgtcct cgcggcgcgg aagctgggcg acaagctgga agcggattac
     2341 gccaagaagg tcaacgaccc ggaagtcaag gcggccctgg ccaggctcaa cgcggccgcg
     2401 gggaagcagt tcaccctcgc ccccaccgcc gccctgatcg ccagcctcaa ggcgctgaag
     2461 cggctggaag acacgatcct ctccgaggcg atcgagctgc ggagcgacgg ccggtcgctg
     2521 tgggtgaaag tcaaggtcga tgagaaatac gaccaggaga tggtcgtgga ttcgggggcc
     2581 agcctggtct gcctgccgca cgcggtggcc gcgaagttcg ggatgaagcc gaccgagaag
     2641 gacccgctgg tgacgctcgt cctggccgac ggccgcgaga ttcagggccg ccggatgaac
     2701 atcaagtcgc tgcgggtcgg ccggttcgag gtggaggacg tggacggcgt cgtcctgggc
     2761 gaggacgctg tcgccgcgca gcccgcgctg ggcctgtcgt tcctggagca cttcaagttc
     2821 gaaatcgata ccggcgctaa gtcgctgacg atggtcaaga tcagcgccga cggcgcagcc
     2881 gcgaaagccg ccaacaaata ggcggccgcc aaaccacgga gatttgtcat gcctgtcact
     2941 cgccgccagt ttctggcaac ttccgcggcc gctgggacaa tggccgcgct gaactttggg
     3001 ccgcggccag cggctgcggc cgaagaaccg gcaatcgcct tcggcctggt gacctacatg
     3061 tggggggcgg actgggacct gccgacgctc ctggccaact gcaagaagac gaacgtgctg
     3121 ggcgtggagc tgcgcacgac gcacgcccac aaggtcgagc cggcgctgaa cgacaagcag
     3181 cgcgcggaag tcgccgccag gttctccgac gccggcgtga cctgcgtcgg catcggcagc
     3241 gacgagcgtt ttgacaatcc cgatccggcg gtcgtgaaga aggcgatcga ggccaccaag
     3301 gagttcgtcc gccttagcca cgacatcggc ggcagcggcg tgaaggtcaa gccgaacgac
     3361 tttcatccga acgtgcccaa agagaagacc atcgagcaga tcggcaaggc cctgaacgag
     3421 ctgggcgaat atgcctcggg cttcgggcag cagatccgcc tggaggtcca cggcaagtgc
     3481 gcggagctgc cgaccatcaa ggcgatcatg gacgtcgcca ccaacgacaa cgtcgccatc
     3541 tgctggaaca gcaacaagac cgacctggcc ggcggcggcc tggaggccaa cttcaacctc
     3601 gtccgcaagc gctggggagc gacctgccac atccacgagc tggaatcggc cgactatccg
     3661 caccagcagc tgatcaacct gctcgtcaag accaagtggg aaggctggct gctcatggaa
     3721 aacgccagca agcccgccga ccgcgtcgcc gccatggccc agcagcggaa gctcttcgac
     3781 gagatgctga aaaaggccgc cggctgaatc tcctgggcac ggccgctaca agcggcagag
     3841 cccgcaaaat cgcgcagctc gccgcgccct ctcgcgcggc cgcacggaga tcgggcgcgg
     3901 gtgatgtatg ctggtctatg agaccagggc cgcctggtgc gccgtttccc cgcgcgcgga
     3961 attgagtgca ggatgccgat catcgaagtt cgccagctga ccaagtccta cgccgatctg
     4021 cggcgggggc agttcgtggc gctggccggc atctcgttcg acgcgcacgc cggacaggtc
     4081 tatggcctgc tcggacccaa cggcgcgggc aagaccagcg ccctgagaat tctcagcacc
     4141 gtcctgcagc cgaccagcgg caccgccaag gtcaacggct gcgacgtgct cacccagccg
     4201 gccctggttc gccgccagct gggcttcgtc tccaccaaca cggccgtcta cgaccggatg
     4261 accgcctggg agatggtcga gtattttggc cggctgaacg gcctggccga agagccgctc
     4321 cgcgagcgga tggaagccct cttcacgcgc ttgcagatga acgacatccg cgacctcttg
     4381 ggcgcgaaga tgtccaccgg catgaagcag aaggtctcga tcgcgcgggc cctggtccac
     4441 gatccgccgc tcctcatttt cgacgaagcg acggcggggc tggacgtgct cgtggcgagg
     4501 aggctcctct tgaccgtggc ccagctgcgc gaccagggca agtgcatcct gttcagcacg
     4561 cacatcatgc gggaggccga gcggctctgc gaccgcctgg ccatcctgca ccgcggccac
     4621 atcctggccg aaggcacgct ctccgagctc cgtgagcggt atcacgagca ggacctcgag
     4681 gagctattct tccgactgat ttcccaacat gacggagaga aggagggact gagagacgga
     4741 gggactgcca acactgccgc tccatcgctc cctctctccc tctctccgtc ccgccgctag
     4801 cctcaccgac ccaccgaccc accgactacc gatcaccgac cgaccgatga actggtccaa
     4861 cgtcaagctg atctacctcc gcgagctgcg cgatcagctg cgcgaccggc ggacgttgtt
     4921 cacgattgcg gtgctgccgc tcttgctcta tccactgatg ggaatggccc tgttgcaggt
     4981 gcagcagttc cgcaaggagc acgcctcgaa ggtgcgaatc attggcgcgg aaagcctgcc
     5041 cgccgagccg cagctgatcg tcggccaaca gttcgccccg catctcaacc agcggctgtt
     5101 ggagctcgag ctgacgcccg cggccggccg ctcgcccacg gaggtacagg ccgaagtcca
     5161 gcgcgagctg cgcgagggcg cgttcgacgc cgccgtctat ttcccgccgg acttcgcccg
     5221 gcggctgcag gactttcgcc ggacgctgcc tgcggccacc ggcgccggcg gcgacgccgg
     5281 gaaggtcccg cagccgcaga tcttcttcaa caccgccagc gacaaatcga aggtggcgac
     5341 gggccgcgtc gaggcggtgc tgcaccagtg gcgcgaggcc atggtgcagc agaacctgaa
     5401 agccagcggc ctgtcgccgg cgacgacgca gccctttgac gtggccagca ccgacgtggc
     5461 ggagcccgcc agccggcggg ccgccctgtg gtcgaaaatc ctgccgttca tcgtgatgat
     5521 ctgggcgctg accggcgcgt tctatccggc gatcgacctg tgcgccggcg agaaagagcg
     5581 cggcacgctc gagacgctgc tctccagccc ggccgcccgc ggcgagatcg tctggggcaa
     5641 gctcttgacg gtgatgacgt tcagcatgct cacggcgctg ctcaacctgg ccgcggtgac
     5701 cgcgatggcg acgttcacga tcagccgcct gcaaaaaatg ggcggcgtga acctgccgtt
     5761 cgaagtcggc ccgccgccct ggaccgccct cgtctggctg gtgctggcgc tgattccgat
     5821 ctcggccctg ttcagcgccc tgtcgctggc gatcgccgcc ttcgcccgca gctcgaagga
     5881 agggcagtat tacctgatgc cgatgctgat gatctcgctg ccgctgatga tgctgccgat
     5941 gctgccggcg atcgagttgg acctgggcta cgccctgatt ccgctgagcg gcgtgctgct
     6001 gctcttgcgg gcgctgatcg aagggcagta tctcgaggcc ctccgctata gcgtgccggt
     6061 ggtgggcgtg acggcggtct gctgcctgct ggcgattcgc tgggcgatcg accagttcaa
     6121 caacgaggcg gtcctgttcc gcgagagcga gcggtttggc ctgggcctct ggatccggca
     6181 cctggtccgc gaccggatgg acacgccgac cgtcggcgag gccctgctct gcggcgtgct
     6241 gatcttgatc gtccggttct tcgccggcct gacgatggcc gaaccgacca actggcgctc
     6301 gttcgttacg agcacgctca cgattcaact cgccttcgtc gccgcgccgg cctgcctgat
     6361 ggcgatcatg ctgacccgca agccgctgct ggcgctttcg ctggcgcggc cgtcgttcct
     6421 ccgcacgatt cccgcggccg gcctgctggc gttgctcctg cacccggtcc tgtactggct
     6481 ggcgcacggc atcgagctgg tctatccgca gagccccggc gtggccgagc ggctggcgga
     6541 gatgctgcag tttgtggacc acgcgccgct ctggcaggcc ttgctgctga tcgcgctggc
     6601 gcccgccatc tgcgaggagt tggcgtttcg cggcttcatc ctctccggcc tgcggcacct
     6661 ggggcacaag tggggcgcga tcgtgctggc cagcgtcttc ttcggcctca cgcacttcat
     6721 gctccagcag tcgatctcgg cctgcgtcat cggcatcgtc atcggctacg tcgcggtcaa
     6781 aacgggcagc atcttccccg gcatgctcta tcacttcgtc cacaacggct cgtcgctgct
     6841 gctgtcgcgc gtcacgcctg atctgctcga gagctatccg ctgctcaagc tcgtcttcac
     6901 ccccggcgcg gaacccggct catatttcta tcgcgtaccg ttcacggtcg ccgccgcatt
     6961 gctgggcctg ggcctgctgt ggtggttcca gcgcctgccc tatcaccgct cggcggaaga
     7021 gcgactgcaa gaggcgctcg atcatcagga agtcgggggc caggagccag cggccaggag
     7081 ccagggaccg gccttgctta cgggccacgg gcagtagttc agtcgtattg tcttttttgc
     7141 gtgacagctt gcctcggccg gcgctacaat gccggcgtct tggacttggg gtcccttggg
     7201 tttgcttggg tatcgctggg gtttgcggtt cgtgacgttc cgtgcctgat ggacgatttg
     7261 gggagggcac cggtatgtct cgtgcgctcg ttgcgctttc attgattctc gttctctgcg
     7321 ccgccgtctc gggctgcggc aaagccgctg cgaccagcgg cgagccggag gcgaaagagc
     7381 ggctgaagtg cctgctggag ctctatcggc actacgtctt ggcaaacggc aaggggcccg
     7441 ccaatgagca ggagctgcgg gcgtatggcc agaagctgac gccgcaggac cggacggctt
     7501 tcatgatcgg cgacgacctg gaaaacatct tcgtctcgcc gcgcgacaat cagaaattcc
     7561 aggtgcggta cggactcaag ttggaaccta ccggcgccac gcgggccgtg gcttgggaag
     7621 cgacgccgca gaacggcaag cggtacgtcg ccctgaccat gctctatgtc gaagagtacg
     7681 acgaggaaac gttcaaaagc tataaccact gaccgctcct ccattcggga gtcccggcaa
     7741 tgttcagttc cgtccggtcc cggcgaggct tcacgctcgt cgaattgctc gtggtgatcg
     7801 ccatcatcgg cgtgctggtc gcgctcctct tgcccgcggt ccagtcggcc cgcgaggcct
     7861 cgcggcggac caagtgcatg aacaacctca agcaactggg gctggccatg cacaatttcc
     7921 acgacgtcta tctccgcttt cccagcagcg gctggtacga ctggtgccgg gcgatgccca
     7981 acgccgtgcc gaccggcatg agcgccggcg attggggcca gaacggctgc atcgtcgcct
     8041 acggtaacgt caactccttc agtaacggcc ccgtcgtcgc caatcaaccg acgggccagc
     8101 cctggcccgc cccgccgcag caggccgcgg gctggccctt ccagctcttg ccctacgtcg
     8161 agcagaacgc ggcccagaac ctgggcggcg ggcatatccg caataccggg ctccccgcat
     8221 acgtttgccc atcgcggcgg ccgccgagcc agcggctgac gctgggcagc gcgctgggcg
     8281 cgaagcccaa ctgctacgcc acgccgtatt tcggcccggt gtcgcgcgac gccaacacga
     8341 ttcgcaatac cgaggcgagc tttttcggga tcatcgtgcc ctccgagccg cccgccgcga
     8401 ataccggccg cggcgaccac gtcgtgcgga tggcctcgat caccgacggc accagcaaca
     8461 cgctgctcct gggcgagaag tggctgcggc ccgatcaata cctgacgggc gcctggatgg
     8521 acgaccacaa cttcgccagc gcgcacgatc aggaccacct gcggattgcc gacgagccac
     8581 cagtcaagga cacgaacaac aatccgctaa ccggagtccg cgtcgcacag ggcgacaaca
     8641 acccctgctg cgattattgg cgcgacgcat tcactcgtaa cccatcgccg cggctgggca
     8701 gctacttcgg cggccctcat cccggcggaa tgaactgcgt gctcgccgac ggcagtgtcc
     8761 gcgggatcaa ctggaccatc ac
//
LOCUS       DASRJB010000183         5976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_614754, whole genome shotgun sequence.
ACCESSION   DASRJB010000183 DASRJB010000000
VERSION     DASRJB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614754"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1374
                     /locus_tag="VFB80_05145"
     CDS             97..1374
                     /locus_tag="VFB80_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN33179.1"
                     /translation="MPNWMHLTAAAVLTALGARAAAAEPASRPNIVILLSDDQAWSDY
                     SFLGHPAIKTPHIDKLASQSAVFTRGYTPMSLCRPSLMTIISGLYPRQHLVTGNDPPM
                     GTDRREMLRHVRRLPTLPKLLAEQGYVSFQSGKWWEGNYAEGGFTAGMTHGDPTKGGR
                     HGDEGLKIGRQGLQPIYDFLAANKGKPFFLWYAPMLPHTPHNPPERLLAKYRDKTDSL
                     FVAKYWANCEWWDETCGELLAHLDQQGLSENTLVVYVTDNGWIQNPAANAYAPRSKRS
                     PNEGGIRTPIMLRWPAKLKPGRDDRTLVGTIDLAPTILAACGLPPGKEMRGINLLDVL
                     LGGLLDGAAGKKPARDTLFGEIYGHDVIDIDRAEPNVLQRWCISGDWKLIEMPDGTLP
                     ELYNLAADPAEAKNLAADQPARVKELSGKIKAQ"
     gene            complement(1468..2094)
                     /locus_tag="VFB80_05150"
     CDS             complement(1468..2094)
                     /locus_tag="VFB80_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN33180.1"
                     /translation="MVSQGSKSVPREAVVLVHGLAANRFIMAPLERALASCFGKVLNW
                     GYPSLWSRIEQHGTSLADFLRLLDCHAYERIHLVTHSMGGIVARLALGLFTPRRLARF
                     VMITPPNRGSHIASRLAPYLGRICPPLVQLADHDASFVRSLPPPQVPELGIIAADADF
                     LVLEPNTRLGCERDHITVPGLHSTLLWRRETFEQVRHFLEHGRFHRAA"
     gene            2262..3128
                     /locus_tag="VFB80_05155"
     CDS             2262..3128
                     /locus_tag="VFB80_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZN33181.1"
                     /translation="MTATSATQLIRVGHSPDPDDAFMFYALAKDRIDTGRFRFEHELV
                     DIETLNRRAFSGELELTALSLHAYAYLADKYVLCTCGASMGDNYGPMVVAREECSIES
                     LRGKTIAVPGTLTTAYLTLRMCIGNDFQHLVVPFDQILDVVAAGEHAGRKIDAGLIIH
                     EGQLTYGEQGLRLVVDLGKWWMSQTGLPLPLGANGVRKDLGTDAIREINRLLKESIAY
                     GLAHRRPALAHALQFGRGLDTGKADTFVGMYVNDWTLDFGERGRKAVKELLARGHAAG
                     VIPKLVEPEFVA"
     gene            3264..3773
                     /locus_tag="VFB80_05160"
     CDS             3264..3773
                     /locus_tag="VFB80_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33182.1"
                     /translation="MAPTTLVRRPSAGTHLPPSGQPRRFIAADSWSAERPEALVICCS
                     DGRWHGQIEEFVRSQVSQRADLMALPGGPAMLNRWTSSVEEARVAEQALAFLCKHHEL
                     SSAWLIAHQDCAYYAAKYGPLDGAYQLRRQMEDLEKGAEILRRLQPNLAIHLVFSARE
                     GDTVVFTSR"
     gene            3816..4112
                     /locus_tag="VFB80_05165"
     CDS             3816..4112
                     /locus_tag="VFB80_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33183.1"
                     /translation="MDPLAQPAGKITSPPMPPRPDPPEKPRGTWLAIVLMGSGALALI
                     VVLTFLTLGFFGPLVLIGLGVFLIIGLQYLLWGWWFERIYRSQASDEEVRPRPD"
     gene            complement(4125..4637)
                     /locus_tag="VFB80_05170"
     CDS             complement(4125..4637)
                     /locus_tag="VFB80_05170"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN33184.1"
                     /translation="MDDNPYRAPEADGYERPEGAPYVGRAGSGRFLAAQIDHAFGVVI
                     FLAVGMNIAETVGNVGAGLIALAAYLGYYLVPEYLFGATVGKSLFALQVRCTSGKRCT
                     LRQVAIRTALRLVEVNPLLLGAIPAGIAILSTQRKQRIGDLLAGTVVVRYTDLTQANP
                     AGPAIKIVRR"
     gene            complement(4664..5257)
                     /locus_tag="VFB80_05175"
     CDS             complement(4664..5257)
                     /locus_tag="VFB80_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN33185.1"
                     /translation="MVKTASTMKKLGTPAPDFKLTNVDGRLVGLHDFDSQPALLVIFM
                     CNHCPFVKHVADGLTRLASDYLPKGVAIVGINANDAAAHPQDSPEQMVHEAEQRGYPF
                     PYLYDETQEVAKAYGAACTPDFFLFGKNRQLYYRGQLDASRPGNNIPVTGSDLRAALD
                     ALLAGKPPPDKQTPSIGCNIKWRAGHEPAYYDPVGVK"
     gene            5293..5685
                     /locus_tag="VFB80_05180"
     CDS             5293..5685
                     /locus_tag="VFB80_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33186.1"
                     /translation="MRESYPPSADANSPAPPARPEPPPEKFQFSLKQLLAFMLASAVL
                     ATGVRYVVQLFGQIPDSRIAGYLYLLVLSLVFGAFLYFLIRGPFLLVHAARFSRRWRT
                     IRGHRRDLEQWSRERIKQREPAVEPPVE"
     gene            5707..>5976
                     /locus_tag="VFB80_05185"
     CDS             5707..>5976
                     /locus_tag="VFB80_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33187.1"
                     /translation="MPLTNEPPRQFSLAWLIFWTLICAVGAGIARMFVERGQAGIDYL
                     VIAVALVIWLGITWLAILHRVFSRRRWEKVTARRRELEVWSRERLK"
BASE COUNT         1040 a         1897 c         1975 g         1064 t
ORIGIN      
        1 ccaattgcaa ttcctatttt tccaaaatta atctactggc ggcccggcga gccggttacc
       61 cacaatgcac ctaccaccca ccggagactt cgcttcatgc cgaactggat gcacctgacc
      121 gccgcggctg tgctgacggc cctgggcgcg cgggccgcgg cggcagaacc cgccagccgg
      181 ccgaacatcg tgatcctcct gtccgacgac caggcttgga gcgactacag ctttctcggg
      241 catccggcga tcaagacgcc gcacatcgat aagctggcca gccagagcgc cgtgttcact
      301 cgcggctaca cgccgatgag cctgtgccgg ccgagcctga tgacgatcat cagcgggctc
      361 tatccgcggc agcacctggt gaccggcaac gatccgccga tgggcaccga ccgccgcgag
      421 atgctccggc acgtccgccg gctgcccacg ctgccgaagc tgctggccga gcaaggctac
      481 gtcagcttcc agagcggcaa atggtgggaa ggcaactatg ccgaaggcgg cttcaccgcg
      541 ggcatgacgc acggcgatcc gacgaaaggg ggccgccatg gcgacgaagg tttgaagatc
      601 ggtcgccaag gcctgcagcc gatctatgac ttcctcgccg cgaacaaagg caagccgttc
      661 ttcctctggt acgccccgat gctgccgcac acgccgcaca atccgcccga gcggttgctg
      721 gccaaatatc gcgacaagac cgattcgctg ttcgtcgcca aatactgggc caactgcgag
      781 tggtgggacg agacttgcgg cgaattgctc gcgcatctgg accagcaggg cctgagcgag
      841 aacacgctgg tcgtctacgt caccgacaac ggctggatcc agaatcccgc ggcgaacgcc
      901 tacgctccgc ggagcaagcg ctcgcccaac gaaggcggca tccgcacacc gatcatgctc
      961 cgctggccgg caaaactaaa gcccggccgc gacgaccgga ctctggtcgg cacgatcgac
     1021 ctggcgccga ccatcctggc cgcctgcggc cttccgccgg gcaaggagat gcgcgggatc
     1081 aacttgctcg acgtgttgct tggcgggttg cttgacggcg ccgccggcaa aaagcctgct
     1141 cgcgacacgc tcttcggcga gatctacggc cacgacgtga tcgacatcga tcgggccgag
     1201 ccgaacgtgc tgcagcgctg gtgcatttcg ggcgattgga aactgattga aatgcccgac
     1261 ggcacgctgc ccgagctgta caacctcgcc gccgatccgg cggaagcgaa gaacctcgcg
     1321 gccgatcagc cggcgcgagt gaaggagttg agcgggaaga tcaaggccca gtagtgatcg
     1381 tagtagggaa tgccctctgt ggcattcccg cggcacgtga ggtcccgccc aaagggaacg
     1441 ccacgaaggg tgtttcctac agcgcgacta ggccgcccgg tggaatcgcc cgtgctcgag
     1501 aaaatgccgc acctgctcga aggtctcgcg ccgccagagc agcgtcgagt gcaatcccgg
     1561 cacggtgatg tggtcccgct cgcagcccag gcgcgtgttc ggctccagca cgagaaagtc
     1621 tgcatcggcg gcgatgatgc ccagctcggg cacctgcggc ggcggcagcg agcggacgaa
     1681 gctcgcgtcg tgatcggcca gttgcaccag cggcgggcag atgcgtccca ggtaaggcgc
     1741 cagccggctg gcgatgtgcg agccgcgatt gggcggggtg atcatcacga accgggccag
     1801 ccgccgaggc gtgaacaggc ccagcgccag ccgggcgacg atgccgccca tgctgtgggt
     1861 gacgaggtgg atgcgttcgt aggcgtgaca atcgagcagg cggagaaagt cggccagcga
     1921 cgttccgtgc tgttcgatac gagaccagag gctcggatag ccccagttga ggactttacc
     1981 gaagcagctc gccagggccc gctccagcgg ggccatgatg aatcggttcg ccgccaggcc
     2041 gtggacgagg acgaccgcct cgcgcggcac cgacttgctg ccctgactga ccattaacgc
     2101 ccttcctcca cacgctctat cgatatttta gttcgaagtt ttcccgtggc tagtttagcc
     2161 agagcgttaa accaccctca gatttgacgg ttagtcggtg gttgccaccc ggaagctggt
     2221 gcggcatgat ggcttaccct cgatttcgct caggaacatg catgacagcg acctcggcca
     2281 cccagctcat ccgcgtcggc cattcgccgg acccggacga cgcttttatg ttctacgccc
     2341 tggccaagga ccggatcgac accggccgct tccggttcga gcacgagctg gttgatatcg
     2401 agacgctcaa ccgccgggca ttttccggcg aattggagct gacggccctc agcctgcacg
     2461 cctatgccta cctcgccgac aagtacgtgc tgtgcacctg cggcgcgagc atgggcgaca
     2521 actacggccc gatggtcgtc gcccgcgaag agtgctcgat cgagtcgctc cgcggcaaga
     2581 ccatcgccgt gccgggcacg ctgaccacgg cgtacttgac gctgcggatg tgcattggca
     2641 acgactttca gcacctcgtc gtgccgttcg accagattct cgacgtagtg gccgcgggcg
     2701 agcacgccgg gcgaaagatc gacgccggcc tgatcattca cgaggggcag ctcacctatg
     2761 gcgagcaggg cctgcgcctg gtggtcgatc tgggaaaatg gtggatgagc cagaccggcc
     2821 tgccgctgcc gctgggggcc aacggggtgc ggaaggacct gggcactgac gccatccgcg
     2881 agatcaaccg gctgctcaag gagagcattg cctatggcct ggcgcatcgg cggccggcgc
     2941 tcgcgcacgc gttacagttc ggccgcggcc tggatacggg caaggccgac acgttcgtcg
     3001 gcatgtacgt gaacgactgg acgctcgact tcggcgagcg cgggcgaaag gcggtgaagg
     3061 aattgctggc ccgtggccac gccgccggcg tcatccccaa gctggtcgag ccggagtttg
     3121 tggcctgact gcaagtcgct tgaatgaccg acggcatcag ggggcggccg ctggtttctc
     3181 gtcatttgtt cgtgtccatt tagccagaaa gtagcgctgt gagcgaacaa gccagggaga
     3241 ccgcgtctgg cgaggagttt cccatggcac cgaccacgct ggttcgtcgt ccctcggccg
     3301 gcacgcacct gcctccgtct gggcagcccc ggcgattcat cgctgccgac tcctggtcgg
     3361 cggagcggcc ggaagcgctg gtgatctgct gttccgacgg ccggtggcac gggcagatcg
     3421 aggagttcgt ccggtcgcag gtttcccagc gggccgatct gatggccctg cccggtgggc
     3481 cggcgatgct caaccgctgg acgtcgtcgg tcgaggaagc ccgggttgcc gagcaggcgt
     3541 tggcgtttct ctgcaagcat cacgagctct cgtccgcctg gctgatcgcg catcaggact
     3601 gcgcctatta cgcggccaaa tacggcccgc tcgacggcgc gtatcagctg cggcgacaaa
     3661 tggaggactt ggagaagggg gcggagatcc tgcgccgcct ccagcccaac ctcgctattc
     3721 acctcgtgtt cagcgcacgg gaaggggaca cagtcgtgtt cacgagccgc tgaggggcga
     3781 tggctcagtc ggcgtctgtc gcgtaaagtg cggcgatgga tcctctcgcg cagcccgccg
     3841 gcaaaataac atcgccgcca atgccgcctc ggcccgatcc gcccgaaaag ccccgcggca
     3901 cctggctggc gatcgtgctg atgggttcgg gcgcgctggc cctgatcgtc gtcctgacct
     3961 tcctgacgct gggttttttc ggcccgctcg tcctgatcgg cctgggcgtg ttcttgataa
     4021 tcggcctcca gtacctgctg tggggctggt ggttcgagcg gatctaccgg tcgcaggcat
     4081 ctgacgaaga ggtgcggccc cgaccggatt gatcgccggg ccggctagcg acgaacgatc
     4141 ttgatcgcag ggcctgccgg gttcgcttga gtcaggtcgg tgtatcggac caccaccgtt
     4201 ccggccagca gatcgccgat gcgctgcttg cgttgggtcg aaagaatggc gattcctgca
     4261 ggaatagcgc ccagcagcag cggattgact tccaccaggc gcagagccgt tcgaatggcg
     4321 acctggcgga gcgtgcatcg cttccccgag gtgcagcgga cttgcaacgc aaacagcgac
     4381 ttccccaccg tcgccccaaa caggtactcc ggaacaaggt agtagccgag gtaggccgcc
     4441 agggcgatca gtccagcgcc gacgtttccc acagtttcgg caatgttcat tccgacagcc
     4501 aggaatatta ccacgccgaa ggcatgatcg atctgggccg cgagaaagcg gccgctgccc
     4561 gcccggccga catagggtgc gccttcaggt cgctcatagc cgtccgcttc cggcgctcga
     4621 tagggattgt cgtccacttg gcgttgatgg attggtcgcg atgttatttc actcccacgg
     4681 gatcgtaata cgccggctcg tggccggcgc gccacttgat gttgcagccg atgctgggag
     4741 tttgcttatc gggcggcggt ttgccggcga ggagcgcatc gagcgcggcg cgcaggtcgc
     4801 tgccggtcac ggggatgttg ttgccggggc ggctggcgtc gagctggccg cggtaataga
     4861 gctggcggtt tttgccaaac aggaagaagt cgggcgtgca ggccgcgcca taggctttgg
     4921 ccacctcctg cgtctcgtca tagagatacg ggaacggata accgcgctgc tcggcttcgt
     4981 ggaccatctg ctcgggcgag tcctgcggat gggccgcggc gtcgttggcg ttgatgccga
     5041 cgatggccac gcccttgggc agatagtcgc tcgccagccg cgtcaggccg tcggcgacgt
     5101 gcttgacgaa gggacagtgg ttgcacatga agatgacgag cagcgcgggc tggctgtcga
     5161 agtcgtgcag gccgacgagc cgcccatcga cgttggtcag cttgaaatcg ggggctggag
     5221 ttcccagttt cttcatcgtg ctggcggttt tgaccataga atgaaagctc cccgcgagtg
     5281 accgattgtc cgatgcgcga atcttatccg cccagcgccg acgccaacag ccccgcaccg
     5341 cccgcgcggc ccgagccgcc gccggagaag tttcagttca gcctgaagca gctcctggcg
     5401 ttcatgctgg ccagcgcggt cctcgcgacc ggcgtgcgct acgtggttca gttgttcggc
     5461 cagattcctg attcgcgcat tgccggctac ttgtatctcc tcgtcctgtc gctagtgttt
     5521 ggcgccttcc tctatttcct gattcgcggg ccgttcctgc tggtgcacgc ggcgcgtttc
     5581 agccggcgct ggcggacgat ccgggggcac cgccgcgacc tcgaacagtg gtcgcgcgag
     5641 cggatcaagc agcgagagcc agcggtggaa ccgccggtgg aatgaatcgc gcaggattgt
     5701 gccaagatgc cgctgaccaa cgaaccgccg cgccaattca gcctggcctg gctgatcttc
     5761 tggacgttga tctgcgccgt cggggcagga atcgcgcgga tgtttgtgga gcggggccaa
     5821 gcggggatcg attacctcgt gatcgccgtc gccctggtga tctggctcgg cattacgtgg
     5881 ctggccattt tgcaccgcgt cttttccaga cggcggtggg aaaaggttac agcccggcgt
     5941 cgcgagctcg aggtttggtc gcgcgagcgc ttgaag
//
LOCUS       DASRJB010000184         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1744213, whole genome shotgun sequence.
ACCESSION   DASRJB010000184 DASRJB010000000
VERSION     DASRJB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744213"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFB80_05190"
     CDS             <1..732
                     /locus_tag="VFB80_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN33188.1"
                     /translation="VIRFFQRHQLATGGENWRPTLLRMLLAACLSGVALLGTWGSTQW
                     APTWADKLAESEYQAQLQTAARERGLQSVAPVRPKAKEHTQIWLAIGAIVGTIAAAMM
                     GDWLGRRPAYFLLCALSLASVYGLFLLNKGFGSPLLFWTFLAGTMTASFYGWLPLYLP
                     ELFRTNVRATGQGFGFNFGRILAAIGVLQTGNLMGLFQADAVIAGITIPHGYPLACST
                     MSLVYLIGMVIIWFAPETKGQPLPE"
     gene            747..1382
                     /locus_tag="VFB80_05195"
     CDS             747..1382
                     /locus_tag="VFB80_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33189.1"
                     /translation="MSPQSQRPWFRLHWLTLIVLSWLIGAIADCQLQKQSCDLYGDYE
                     KWDTAFVCGWPFRHLLVRDFNPNRDSVLRLFSLAPTDRHQWYGWALAINGVVWLMLVG
                     CTAWVIERWMRFPKWLQFGSRSLLAFTGVAAVLAAILTQPTPYWVLRLSIEPSFEVEW
                     CNIRKPLRLIIPMGIGCATYFWGWLIVSAAIAGWALVFRRHPKLEPGSTPA"
     gene            1493..2383
                     /locus_tag="VFB80_05200"
     CDS             1493..2383
                     /locus_tag="VFB80_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33190.1"
                     /translation="MLKTASFVMGLTCGCVVFTAAAARGIEPPKPENRVEFEVKVHSP
                     WLARVPFLKQVFAQVAEQEACEHKACEHKIECQAGPCPIGVDFAFPTHHGHGPLVLFS
                     HPLAAAPGICQACPPPGVACAAACTAAKCGADCACSAKSAGCDCDDCRCCQECSGASR
                     AKHDRDSLIEHILEISTEKAALEAVLEAHTSFAEERQAMFESLAELTAENAKLTAKLE
                     FQAEREELHKQFQELAAQNAHLKAEVALTEARVKLMQESFQLAVEKQRLEQRVAELER
                     HALQGNVRTAKQPHEIEPAR"
     gene            2507..3163
                     /locus_tag="VFB80_05205"
     CDS             2507..3163
                     /locus_tag="VFB80_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33191.1"
                     /translation="MRYFALFCVALACQLPAALAAEGDIVLFNGKDFTGWTAVLAKPD
                     AKLEDVWSIKDGVLSCKGEPRGYLRTTKDDFADYTLRLQWRFPKGTPGGNSGVLLHTS
                     TPGALGIWPKSIEAQLNHQNAGDIWVIPESTTVQIENAAERVKGRRHLNLTDDSEKPI
                     GEWNDYEIVCNGDQITIKVNGVLVNHVTKCSITKGAICLQSEGAAVEFQKILLTPLVK
                     "
     gene            complement(3144..>4136)
                     /locus_tag="VFB80_05210"
     CDS             complement(3144..>4136)
                     /locus_tag="VFB80_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HZN33192.1"
                     /translation="FPVRMTTHPHDGFQLARCRNAGVRASTADYLVFLDGDCIIPPDH
                     LKVHLDRRRENTVQAGFFARLSEAASARITEEEIRRGNFARWETWSERWKLFKLHAKG
                     EFYRWIGDRTRPKLFGNNIGIARRDYERVNGYDENFRGWGCEDDDLRLRLRAAGIDIV
                     SIVWWARTYHLWHARTPSAPKTWREGHNVNYLLRPVRLTRCLAGLVTRRLRDLHVQVV
                     GRRPPSETLDRTLPLWCREALGSRHNGTAEIELAFAPSSEPFSNSGDCRVLIVPSGQP
                     AARKLVRQSDLIFARGGVPGAAEGKTFAIESLPAILSRNLGLARAAELVTSPAA"
BASE COUNT          689 a         1339 c         1368 g          740 t
ORIGIN      
        1 gtcattcgtt tcttccagcg gcatcaattg gcaacgggcg gcgagaattg gcggccgacg
       61 ctgctgcgga tgctgctggc ggcctgcctg agcggcgtgg ccctgctggg cacttggggc
      121 tcgacccagt gggcgccgac ctgggccgac aagctcgcgg agtcagagta tcaggcccaa
      181 ctgcaaacgg cagctcgcga gcgtggcctg cagtcagtgg ctcccgtccg gccaaaggcc
      241 aaggagcata cgcagatctg gctcgccatc ggagcgatcg tgggcaccat cgcggccgcc
      301 atgatgggcg attggttggg ccggcggccg gcctatttcc tgctctgcgc tctgtcgctg
      361 gccagcgtct atggactgtt cctcctcaac aagggtttcg gctcgccgtt gctcttctgg
      421 acgttcctcg ccggcacgat gaccgcgtcg ttctacggct ggctgccgct ctatctgcct
      481 gagctgttcc gcacgaacgt ccgcgcgacg ggccagggct ttggcttcaa cttcggccgc
      541 atcctggccg ctatcggcgt cttgcagacc ggcaacctga tgggcctctt tcaggccgac
      601 gccgtcatcg ccggcatcac gatcccccac ggctatccgc tggcttgctc gacgatgagc
      661 ctcgtctatc tcatcggcat ggtcatcatc tggtttgccc ccgaaacgaa agggcagccg
      721 ctgccggagt gaaatagaat caaggcatgt cgccgcagtc acagcgcccc tggtttcggc
      781 tgcattggct cacgctgatc gtgctgagct ggctgattgg ggcgatcgcc gactgccagc
      841 tgcaaaagca gagctgcgat ctgtacggcg actatgagaa gtgggatacg gcctttgtgt
      901 gcggctggcc ctttcggcac ttgctggtga gggatttcaa tcccaaccgg gacagcgtcc
      961 tgcggctgtt ttctctggct ccaaccgatc ggcaccagtg gtatggctgg gcactcgcca
     1021 tcaacggtgt cgtctggctg atgctcgtcg gctgcacggc ctgggtgatc gagcgctgga
     1081 tgcgctttcc caaatggctg caattcgggt cgcggtcgct gctggcgttt accggcgtag
     1141 ccgcggtctt ggcggcgatt ctcacgcaac ccacgcctta ctgggtgctg cggctgagca
     1201 ttgaaccgag cttcgaagtc gagtggtgca acatccgcaa gccgctgcgc ctaatcattc
     1261 ccatgggcat cggctgcgcc acttacttct ggggctggct aatcgtttct gccgcgatcg
     1321 cgggatgggc actggtattc cggcgtcatc cgaagctcga gccgggttct acgccggcct
     1381 agcatgcttg ccagcacttc gccgctggca aggattcgtc ttgccgcggc ggaagcccac
     1441 ggctatatcc cgcggccttg tcttgttcca gtgcgccaac caggagagcg ttatgctcaa
     1501 aacagcaagt ttcgtgatgg ggctgacctg cggctgcgtg gtcttcacgg cagcggcggc
     1561 ccgcggcatt gagccgccca agccggaaaa ccgagtcgag tttgaagtga aagtgcattc
     1621 gccgtggctg gcgagggtgc ccttcctgaa gcaagtcttc gcgcaggtcg ccgagcaaga
     1681 ggcctgcgag cacaaggcct gcgagcacaa gatcgagtgc caggcgggcc cctgtccgat
     1741 cggcgtggat ttcgccttcc cgacgcatca cggccatggc ccgctggtgc tgttctcgca
     1801 tccgctggct gccgcgccgg gcatctgcca ggcttgtccg ccgccgggag tggcctgcgc
     1861 cgctgcctgc accgcggcca aatgcggcgc cgactgtgcg tgcagtgcca agagcgcagg
     1921 ctgtgactgc gacgactgcc gctgctgcca ggagtgcagc ggcgctagcc gcgcgaagca
     1981 cgatcgcgac tcgctgatcg agcacatcct ggaaatctcg accgaaaagg ccgcactgga
     2041 ggccgtcctg gaagcgcaca cgagctttgc cgaagagcgg caggcgatgt tcgagtcgct
     2101 ggccgagctg accgccgaga atgcgaagct gaccgcgaag ctggagttcc aggccgagcg
     2161 cgaggagctg cacaagcagt tccaggagct ggcggcgcag aacgcgcacc tcaaggccga
     2221 ggtcgcgctg accgaggccc gcgtcaagct gatgcaggaa tcgttccagc tcgccgtcga
     2281 gaagcagcgg ctggagcagc gcgtcgcgga gctcgagcgg catgccctgc agggcaacgt
     2341 ccgcacggcc aagcagccgc atgaaatcga accggcccgc tagagtggtg cgtgttactt
     2401 tgcgtgtcgc ccgctcgcta tgcgagcggg cgacctcacg cggagcgtga ggactactga
     2461 cgcggccagc gcggaggcgt atgctacggg gaacgaggag ttctccatgc gctacttcgc
     2521 cctgttctgc gttgctctcg cctgccagct gcccgcggcc cttgccgccg agggcgacat
     2581 cgtcctcttc aacggtaagg acttcaccgg ctggacggcc gtgctggcca agccggacgc
     2641 caaactcgag gatgtgtgga gcattaagga cggcgtgctc tcgtgcaaag gggagccgcg
     2701 gggctacttg cggacgacga aggacgattt cgccgactac acgctccgcc tgcagtggcg
     2761 gtttcccaag ggcacgccgg gcggcaacag tggcgtgctc ttgcatacca gcacgcccgg
     2821 agccctcggc atctggccga agtcgatcga ggcccagctc aatcaccaga atgcgggcga
     2881 catctgggtc atcccggaat ccacgacggt ccagatcgaa aacgccgccg agcgggtcaa
     2941 gggccggcgg catctgaatc tgaccgacga ttccgagaag ccgatcggcg agtggaatga
     3001 ctacgaaatc gtttgcaacg gcgatcagat caccatcaag gtcaacggcg tgctcgtgaa
     3061 ccacgtcacc aagtgcagca tcaccaaggg cgcgatttgc ctccagtcgg aaggcgccgc
     3121 ggtcgaattt cagaaaattc ttctcacgcc gctggtgaag tgaccagctc ggcggcccgc
     3181 gccaggccca ggttcctgga caggatcgcc ggcaacgatt cgatcgcaaa cgtcttgccc
     3241 tcggccgcgc cgggaactcc gccccgtgcg aagatcaggt cgctctgccg cacgagcttt
     3301 cgcgccgccg gctggccgct gggaacgatc agcacgcggc aatcgccgct attcgaaaac
     3361 ggctcgctcg agggcgcgaa cgccagctcg atctccgccg tgccgttgtg gcgcgagccc
     3421 agtgcctcgc ggcaccacag cggcagcgtc cggtcgagcg tctcgctcgg cggacggcgg
     3481 ccgacgacct gcacgtgcag gtcccgcagt cgccgcgtga cgagcccagc caggcagcgc
     3541 gtcagccgca ccggccgcag caggtaattg acgttgtggc cttcccgcca ggtcttgggg
     3601 gccgaaggcg tccgggcgtg ccacaggtga taggtgcggg cccaccacac gatcgagacg
     3661 atgtcgatgc cggcggcccg cagccgcagc cgcaggtcgt cgtcctcgca gccccagccg
     3721 cgaaagttct cgtcgtagcc gttgacccgc tcgtagtcgc gtcgggcgat gccgatgttg
     3781 ttgccgaaca gcttgggccg cgtgcggtcg ccgatccagc gatagaactc gcccttggcg
     3841 tggagcttga agagcttcca gcgctcgctc caggtttccc accgcgcgaa gttgccgcgg
     3901 cgaatttctt cctcggtgat ccgcgcgctg gccgcctcgc ttagccgggc aaaaaagccg
     3961 gcctggaccg tgttctcgcg gcggcggtcg aggtgaacct tcaggtggtc gggcgggatg
     4021 atgcagtcgc cgtcgaggaa caccaggtag tccgccgtgc tggcccgcac ccctgcgttg
     4081 cggcagcggg cgagttgaaa gccgtcgtgc gggtgcgtcg tcatccgcac cgggaa
//
LOCUS       DASRJB010000185         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2873742, whole genome shotgun sequence.
ACCESSION   DASRJB010000185 DASRJB010000000
VERSION     DASRJB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873742"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VFB80_05215"
     CDS             <1..152
                     /locus_tag="VFB80_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZN33193.1"
                     /translation="SVKQLKAAIYDYIDEHNEHAKGFAWTATPDEILAKVRRARAALD
                     KTPSA"
     gene            complement(177..1121)
                     /locus_tag="VFB80_05220"
     CDS             complement(177..1121)
                     /locus_tag="VFB80_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZN33194.1"
                     /translation="MSAEIVTLTDAAAGTSAEILVSQGFNCFRFSANVRGRPVEVIYA
                     PPDFAEGTGRPSRGGIPLLFPFPGRIPGTRFRWEGHDYPLEPGDALGNAIHGFALSRP
                     WRLIESEAARVAGQFHARRDDPSLTSRWPADFQITATYQLAGNLLAGHFLFENPSDTP
                     LPCGFGAHPYFRVPIGGPGGDDCVVKLPVSARWELRDMLPTGRMLALPNEAAFAAGQR
                     FGQMQFDDVFTGLAFGGGWCSASIFDPGSGCTVTQRFDGAFRECVVYTPPHRQAICIE
                     PYTCVPGAFALEERGIDAGLRIVPPGGSFAARVEINVT"
     gene            complement(1129..2097)
                     /locus_tag="VFB80_05225"
     CDS             complement(1129..2097)
                     /locus_tag="VFB80_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33195.1"
                     /translation="MFETAITLLLALHLLCVNAASGGPLVAAWLDWRAARGDDAAALA
                     AKFLARHALLGLLLGAILGVTIGWLLWNPAYQSLWTGPLRYKAAGAGIEFAFSFVLLL
                     AWWLWLPAKGGGRRLSAALRGVMAMLATTNLLYHFPVLFSVAARLYDRGQVAGETIRG
                     ASFRELAAFGETPPLALHVTLSSLAVAGVLLLGLALRWRRSGEAAGADRIALWGGRWA
                     LAATLAQLPVGLWTLAVLPTGPQTQIMGASPIGILLFVGSLAAALWLMSELGYVALGE
                     TSRPNLLRAMAALLITIFLMTALQRQTRTPQPAISPGQPAAAVSSP"
     gene            2191..3186
                     /locus_tag="VFB80_05230"
     CDS             2191..3186
                     /locus_tag="VFB80_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN33196.1"
                     /translation="MLSAVIPVLNEAESLPALYGELSDVARENGYDLEIVFVDDGSSD
                     QSWQEIERLAAADRRVRGIRFRRNFGKAAALSAGFAAARGELIVTLDADLQDDPREIP
                     RFLAEIEKGLDCVSGWKQVRHDPWHKVGPSRIFNWLVGALTGVKLHDHNCGFKCYRRE
                     IFDEVRLYGELHRFVPVLAAARGWKVGEIAVNHRARKFGRSKYGVTRIVKGFLDLLTV
                     YFLTGFEQRPQHLLGTLGIGAFAIGLAGLTYLTITWVLANLTDWNQWFPNWQFKPLHE
                     RPAVLYSMGMLLLGAQFISMGFLAELFTAYYGRTSVPYSIKQRAGQSWAEQEARP"
     gene            3183..>4261
                     /locus_tag="VFB80_05235"
     CDS             3183..>4261
                     /locus_tag="VFB80_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33197.1"
                     /translation="MNDAPAARWWIYSLLIVISGGIMLARVMHVQSGEGNNATPFLSA
                     NDRSRWTTIRALGDDQTYSIDHIIFSERGGRIRGWHTIDLVRHRGRDGLQHYYSSKPT
                     LLMTLLAGEYWLVKTLTGATLKDQTFYVARLMLLLTNVLPLVAALVLLAWQIDRLSQS
                     DGARVFAVAAACFATFLTTFAVTLNNHLTAGISLIVGLAAVLPIASGESRAWWRFAVG
                     GLAFGFLASNELPALSMLLLVGVGLLLLSPLRTIAAFVPAALVVAAGAFGTNILAHGD
                     WRTPYAHRSDGPLVATIADGLAVPLDAGLASPELIAALREHRIEVSDQPVIEQRTPGD
                     RWMLWDKTTQAELALRRTPEGELHGG"
BASE COUNT          748 a         1414 c         1431 g          668 t
ORIGIN      
        1 ccagcgtcaa gcagttgaaa gccgcgatct acgactatat cgatgaacat aacgagcacg
       61 ccaagggctt cgcctggacc gctacccctg acgaaatcct ggccaaagtc cgtcgcgcgc
      121 gggctgctct cgataagacc ccatcagctt gaagcactac accaggccct gctgcgtcag
      181 gtcacgttga tctcgacccg ggcggcaaag gagccgccgg gcgggacgat tcgcagtccg
      241 gcgtcgatgc cgcgctcttc cagcgcgaag gcgccgggga cgcaggtgta gggctcgatg
      301 cagatggcct gccggtgcgg cggcgtatag acgacgcact cgcgaaacgc accgtcgaac
      361 cgctgagtca cggtgcagcc gctgccaggg tcaaagatcg acgcgctgca ccagcccccg
      421 ccgaatgcga ggccggtgaa gacgtcgtcg aactgcatct ggccgaagcg ctggccggcg
      481 gcaaacgcgg cttcattcgg cagggcgagc atgcgcccgg tgggcagcat gtccctcagc
      541 tcccagcggg ccgagaccgg cagcttgacg acgcagtcgt ccccgccggg gccgccaatc
      601 ggcacgcgga aataaggatg cgcgccaaag ccgcagggca ggggagtatc ggacggattc
      661 tcgaacagga agtggccggc cagcaggttc ccggcgagct ggtaggtcgc ggtgatttga
      721 aagtcggcgg gccagcggga cgtaagcgag ggatcgtcgc gccgggcatg aaactggccg
      781 gcgacgcgcg cggcctcact ctcgatgagc cgccaaggcc gcgacaaggc aaagccgtgg
      841 atcgcgttgc cgagtgcatc gccgggctcc agcggataat cgtgtccctc ccagcggaac
      901 cgcgtgccgg gaatgcggcc gggaaagggg aacaagagcg gaatgccgcc ccgcgacggc
      961 cggccggttc cctccgcgaa atcaggcgga gcatagatca cttcgaccgg ccggccacga
     1021 acattcgccg aaaagcgaaa gcaattgaag ccctggctga ccaggatttc cgccgacgtg
     1081 ccggctgcgg cgtctgttag cgtgacgatc tcggcgctca tggatgtcct acgggctgga
     1141 gaccgccgct gcgggttgac ctggcgagat tgccggttgc ggcgtgcgag tttgccgctg
     1201 cagggcggtc atcaggaaga tggtaatgag cagcgcggcc atcgcgcgga gcaggttcgg
     1261 ccgcgacgtt tcgcccaggg cgacgtatcc cagctcgctc atcagccaga gggccgccgc
     1321 cagggagccg acgaacaaca agatgccgat ggggctggcg cccatgattt gcgtctgcgg
     1381 tccggtcggc agaacggcga gcgtccacag tccgacggga agctgcgcga gcgtagcagc
     1441 gagggcccag cggccacccc agagggcgat tcgatcggcg ccggccgcct cgccgctccg
     1501 ccgccagcgc agggccaggc ccagcagcaa tactcctgcg acggccagcg aggagagcgt
     1561 gacgtgcagc gccagcggcg gcgtttcgcc aaacgccgcg agctcgcgga agctggcccc
     1621 gcggatcgtc tcgcccgcga cctgcccgcg gtcgtacaac cgcgccgcca ccgagaagag
     1681 cacgggaaag tgatagagca gattggtggt ggccagcatg gccatcactc cgcgcaaggc
     1741 ggccgacaag cgccgtccgc cgcctttggc cggcagccac agccaccagg ccagcaggag
     1801 cacgaatgag aatgcgaact cgatgccggc gccggcagcc ttgtagcgga gcggccccgt
     1861 ccacagcgac tgataagcag gattccaaag cagccagccg atcgtcacgc ccaagatcgc
     1921 cccgagcagc aggcccagca aagcatgccg cgccaggaac ttcgccgcaa gagccgccgc
     1981 gtcgtcgccg cgcgcggctc gccagtcgag ccaggcggca acgagcggcc cgccgctggc
     2041 cgcgttcacg cacagcaggt gcaaggcgag cagcaaggtg atggccgttt cgaacacagt
     2101 cgtttctgat ttgaggtgcg aatcgccgcc cgccggccgc gggtttggat tattctagag
     2161 aaccacgccc ctgggaaaac cgccgctcgc atgctctcag ccgtcattcc cgtcttgaac
     2221 gaagccgaga gcctgccggc gctctacggc gagctgtccg acgtagcccg cgagaacggc
     2281 tacgacctgg agatcgtctt cgtcgatgac gggtcgagcg atcagtcgtg gcaggaaatc
     2341 gagcgcctgg ctgcggccga tcgccgggtc cgcggcatcc gcttccggcg gaatttcggc
     2401 aaggcggcgg ccctgtcggc cggctttgcc gcggcgcggg gcgaactgat cgtaacgctc
     2461 gacgccgacc tgcaggacga cccccgcgag attccgcggt ttctcgccga gatcgagaag
     2521 ggactcgact gcgtcagcgg ctggaagcag gtccgccacg atccctggca caaggtcggc
     2581 ccgtcgcgga tcttcaactg gctcgtcggc gcgctcaccg gcgtcaagct gcacgatcac
     2641 aactgcggct tcaagtgcta ccgccgcgag atcttcgatg aagtccggct gtacggcgaa
     2701 ttgcaccgct tcgtgccggt gctggccgcc gcccgcggct ggaaggtcgg cgagatcgcc
     2761 gtcaaccatc gggcccgcaa gttcggccgc tcgaagtacg gcgtgacgcg gatcgtgaaa
     2821 ggcttcctcg atttgctcac cgtctatttt ctcacgggct tcgagcagcg gccgcagcac
     2881 ctcttgggaa cgctcggcat cggcgcattt gcgatcggcc tggccgggct gacttacctg
     2941 acgatcacct gggtgctcgc caacttgacc gactggaacc agtggtttcc gaattggcag
     3001 ttcaagccgc tgcacgagcg gcccgcggtg ctgtactcga tgggcatgct gctcctgggc
     3061 gcgcagttca tctcgatggg ctttctggcc gagctcttca ccgcctatta cggccgcacg
     3121 agcgttccct attccatcaa gcagcgggcc ggccagtcgt gggccgagca ggaagccagg
     3181 ccatgaacga cgctcccgcc gcccgctggt ggatctacag cctgctgatt gtgatctcgg
     3241 gtggcatcat gctggcccgc gtgatgcacg tgcaatcggg cgagggcaac aacgcgacgc
     3301 cgttcctttc ggccaatgac cgcagccgat ggacgacgat tcgcgcgctg ggcgacgacc
     3361 agacctactc aatcgaccac atcatcttca gcgaacgggg cggccggatt cgcggctggc
     3421 acacgatcga tctggtacgg caccgcggcc gcgacggcct gcagcattac tactcgagca
     3481 agccgacgct cctgatgacg ctgctggccg gcgagtactg gctcgtgaaa acactgaccg
     3541 gcgcgacact caaggatcag acgttctacg tcgcccggct gatgctcctg ctgacgaacg
     3601 tactgccgct ggttgcggcg ctggtgcttc tcgcatggca aatcgaccgg ctgagccaga
     3661 gcgacggggc gcgcgtcttt gccgtcgccg cggcctgttt cgcgactttt ctcaccacct
     3721 tcgcggttac gctcaataac catctgacgg ccgggatcag cctgatcgtg ggcctcgctg
     3781 ccgtgctgcc gatcgctagc ggcgaaagtc gcgcctggtg gcggttcgcc gtcggtggac
     3841 tggcgttcgg ctttctggca tcgaacgagt tgccggccct gtcgatgctg ttgctcgtcg
     3901 gcgtggggtt gctgttgctg tcgccactgc ggacgatcgc ggcctttgtt ccggcggctc
     3961 tcgttgttgc ggcgggagca ttcggcacca acatcctggc tcacggcgat tggcgcacgc
     4021 cgtacgccca tcgcagcgac ggcccgctcg tggcgaccat cgccgacggc cttgccgtgc
     4081 ccctcgatgc gggcctggcg tcgccggagc tgatcgccgc cctgcgcgag cacagaattg
     4141 aagtctccga ccagccggtc atcgagcagc gcacgccagg cgaccgctgg atgctgtggg
     4201 acaagacgac gcaggcggaa ctcgcgctgc ggcgcactcc agagggcgag ctgcacggcg
     4261 g
//
LOCUS       DASRJB010000186         4764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3719427, whole genome shotgun sequence.
ACCESSION   DASRJB010000186 DASRJB010000000
VERSION     DASRJB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719427"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFB80_05240"
     CDS             <1..1129
                     /locus_tag="VFB80_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33198.1"
                     /translation="TVFFEVADTSPALADLVDPATPDDRLAHRRADFIDEPERIRLQR
                     HLQARLDDYQAQARGDPRIPKFASVFVTDRVGTQLAAAFDGTEANSPGKSVGFNVARR
                     PYFSGNSEGEEQLRPPPNIVHIEQTHLSAVFFSKTQRTWKVAVSTPIYRDPVNREQFD
                     GILVLTISLADFEVSTAALSSSDQFLVLVDGQGRDAGTILHHPLFKELKGQDREIPKE
                     LVGASDPQEVSSKYRVDPALLTEQQSADYVDPIGKYPDPLFKAYDRRWLAAAAPVLPP
                     MGAGEKSESGLVVLMQSDRQAVVMPARNLGAQFMRNSFWMLVVMVTISLALWYIVVRL
                     FREPSAKLNRAATPIESTPLQSMATIAARKEPKNRRTEEPN"
     gene            1171..3021
                     /locus_tag="VFB80_05245"
     CDS             1171..3021
                     /locus_tag="VFB80_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN33199.1"
                     /translation="MPDLIAQGPQPPMRWRRKLYAGAIHVLGRQAGSWSVPWDDRISR
                     RHVEIEYVDGRLRVTALEGARNPIFYRGHKTSDFQVRAGEHFVIGETTFTLVEERVHV
                     SLDLPRPADERTFTLEELRRQPFRQADKRIDVLSRLPDIIGGSATDQELFVRLVNLLL
                     GGIEPASAAAVVALKSATSGRAGEGSGFGVQGSGAAGARGQEPGSSDPRLKSDSVEVL
                     HWDRRVLAGSEFRPSERLIRQAIESGQSVVHAWSPAAKDGEFTVSEGVDWAFCTPVPG
                     RACEGWALYVTGGFPADLAAIRPPDAESLRDEVKFAELTATTLSALRESRLLATRQAG
                     LAQFFSPVVLDALASQEADEALAPREADVVVLFCDLRGFTQQSERAAGDLFGLLNRVS
                     QALGVMTHHILAQGGVVGDFHGDAAMGFWGWPLPQADAVERACRAALGIRSDFVSEAV
                     LADFRVGIGIASGRAVAGKIGTVDQVKVTVFGPVVNLASRLETMTRQLHASILIDPPT
                     AAVIRASLPRELCRVRRLAKVQPAGLSAPLEVSELLPPAGANELTDADVAAYEKALDA
                     FQSRDWQLAHKHLHAVPPDDLAKDFLTIFIARHSRTPPPNWDGTIPLEGK"
     gene            3112..3585
                     /locus_tag="VFB80_05250"
     CDS             3112..3585
                     /locus_tag="VFB80_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HZN33200.1"
                     /translation="MSRAATAELAPLVKLLADDRRYKIEAYHFVEAGLGYAQEVLGLG
                     SSGPRTRRKKKADLGLADDRPVRHVSGQDLCQALRLLALDQYGYLAKLVLASWGIHST
                     GDFGEIVYNLIKIGKMSKSDTDRRNDFENVFEFDQAFVRDFVITKEEESCRPSSP"
     gene            3564..4613
                     /locus_tag="VFB80_05255"
     CDS             3564..4613
                     /locus_tag="VFB80_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HZN33201.1"
                     /translation="MSPLQSLIASGTKLWLDSVDPDLVRSNRALGATGATSNPVIVSD
                     LIKTGRFDRDLERLVAAGKSNDEVAWQLTDQLVRQAQEVFLPVCRETRGNDGYVSFEL
                     EPLLEDPQLHLPLEERTKRYVELGKQWSAGHQNRMIKVPATPAGISALEPLAAAGVTL
                     NVTLVFTPRQYHAAREAIWRGAQKRSSLAGFKSVYSIFVSRVDIYTEKHVPQLSPAAQ
                     GLVGIVNAKQIWADNQAWWKDKKLPLAQEIIFASTGTKKPSDPPWKYVEAFAGSDIQT
                     NPPATNDAVEKSGRTFTRQVDKPPPQPVLAEIAAKVDIQKLEDVLMDEGIQKFADPQK
                     ALLKLIAEKRAALAK"
BASE COUNT          849 a         1590 c         1548 g          777 t
ORIGIN      
        1 cacggtgttc ttcgaggtgg ccgacacaag cccggcgctg gccgacctcg tggatccggc
       61 cacgcccgac gaccggctcg cgcatcgacg ggccgacttt attgacgagc ccgagcggat
      121 tcgactgcag cggcacttgc aggcgcggct tgacgattac caggcccagg cccgtggcga
      181 cccgcggatt ccaaaattcg ccagcgtttt cgtcaccgac cgcgtgggca ctcagctcgc
      241 ggctgcgttc gacggcacgg aggcaaattc gcccggcaag tccgtcggct tcaacgtggc
      301 gcggcgccct tacttcagcg gaaactcgga gggcgaagag caactgcggc cgccgcctaa
      361 catcgtccac atcgagcaaa cgcacctgtc ggcggttttc ttcagcaaga cgcagcggac
      421 ttggaaagtc gccgtgtcga ctcccatcta tcgcgaccca gtcaatcgcg agcagttcga
      481 cggcatcctc gtgctcacga tcagcctggc cgacttcgaa gtctcgaccg ccgccctttc
      541 gtccagcgat cagttcctgg tgctggtcga tggccaaggc cgcgacgccg gcacgatcct
      601 gcaccacccg ctcttcaagg aactcaaagg gcaggaccgg gagatcccca aggagctggt
      661 cggcgcgagc gatccgcagg aggtcagctc caaataccgc gtggaccccg cgctgctcac
      721 tgaacagcag tcggcagact atgtcgatcc gatcggcaag tatcccgatc cgctctttaa
      781 ggcgtacgac cgccgctggc tggcggccgc cgcgccggtg ctgccgccta tgggagccgg
      841 cgaaaagtcg gaatcgggcc tcgtcgtcct gatgcaatcc gatcgccagg ctgtcgtgat
      901 gccggcccgc aacctggggg cccagttcat gcggaacagc ttctggatgc tggtcgtgat
      961 ggtcacgatc agcttggctt tgtggtacat cgtcgtccgc ctgttccgcg agcccagcgc
     1021 caagctgaac cgcgcggcga cgccgatcga atcaacgccg ctgcagagca tggcaaccat
     1081 cgcggcgagg aaagaaccaa agaaccgaag aaccgaagaa ccaaattgac cgcacttcgg
     1141 ttcttcggtt ctttggttct tggattttgt atgcccgacc tcatcgccca gggaccgcag
     1201 ccgcccatgc gctggaggcg caagctctat gccggggcga ttcacgtcct cggccggcag
     1261 gctggctcct ggtccgttcc ctgggacgac cgcatctcac ggagacacgt cgagatcgaa
     1321 tacgtcgatg gccggctgcg ggtcacagcc ctggaggggg cccgcaaccc gatcttctac
     1381 cgcgggcaca agaccagtga cttccaagtg cgggccggcg agcattttgt cattggcgag
     1441 acgaccttta cgctcgtcga ggagcgggtg cacgtctcgc tcgatctgcc gcggccggcc
     1501 gatgagcgga ccttcacgct cgaggagctc cgccgccagc ccttccgcca ggccgacaag
     1561 cggatcgacg tcctgagtcg gctgcccgac atcatcggcg gctctgccac tgaccaggag
     1621 ctgttcgtgc gactggtcaa cctgctgctg ggcggcattg agccggccag cgccgccgcg
     1681 gtggtcgccc tcaaatctgc gaccagtggt cgcgcggggg agggttctgg gttcggggtt
     1741 cagggttcgg gggcggcagg agccaggggc caggagccag gaagcagcga cccaagactg
     1801 aaatccgatt ccgtcgaggt cctgcactgg gatcgccgcg tcctggctgg cagcgagttt
     1861 cggccgagcg agcggctgat ccggcaggcg atcgagtcgg gccagagcgt ggtacacgcc
     1921 tggagcccgg cggcgaagga cggcgagttc acggtcagcg aaggggtcga ttgggcattc
     1981 tgcacgccgg tccccggccg ggcctgcgaa ggctgggcgc tgtacgtcac cggcggcttt
     2041 ccggccgacc tggccgcgat ccgaccgccg gacgccgaat cgcttcgcga cgaagtcaag
     2101 ttcgccgagc taacggcgac cacgctcagc gccctgcgcg agtcgcggct cctcgccaca
     2161 cggcaggcgg gcctggccca gttcttctcg ccggtcgtgc tcgacgcgct ggcctcgcag
     2221 gaggccgacg aagccctcgc gccgcgcgag gccgacgtgg tcgtgctgtt ctgcgacctc
     2281 cgcggcttca cgcagcaaag cgagcgggcc gcgggcgacc tgttcggcct cttgaatcgg
     2341 gtcagccagg cgctgggcgt gatgacgcac cacatcctcg cgcagggcgg cgtcgtcggc
     2401 gactttcacg gtgacgcggc gatgggcttc tggggctggc cgcttcctca ggccgacgct
     2461 gtcgagcggg cctgccgggc cgcgctgggc attcgctcgg atttcgtcag cgaagcggtc
     2521 ctggcggatt ttcgcgtcgg catcggcatc gccagcggtc gcgccgtagc cggcaagatc
     2581 ggcaccgtgg accaggtgaa ggtcaccgtc ttcggcccgg tcgtgaatct cgcgtcgcga
     2641 ttggagacga tgacgcggca acttcacgct tcaatcctga tcgatccgcc gacggccgcc
     2701 gtgatccgcg cttcgctgcc gcgcgagctg tgccgcgtcc gccggctcgc caaggtccag
     2761 cccgccggcc tctccgcgcc gctggaggtc agcgagctgt tgccgccggc cggagcgaat
     2821 gaactcacgg acgccgacgt cgccgcctac gaaaaagccc tggacgcctt tcaatcccgc
     2881 gattggcagc tcgcccacaa gcacctgcac gccgttccgc ccgacgacct ggccaaggat
     2941 ttcctcacca tcttcatcgc ccgtcacagc cgcacgccgc ccccgaactg ggatggaacg
     3001 attccgctgg aaggcaaata ggtcgccgcc gacggctagc cgtgcgcggg ctcggcagct
     3061 actgtattgc tggcgttttt aggaagattc agaaacacgt tggaatgtgc cgtgtcacgc
     3121 gctgctactg cggaactcgc gccgctcgtc aagctgctgg ctgacgaccg gcgatacaag
     3181 atcgaagcct atcatttcgt cgaagccggg ctcggatacg cccaggaggt gctgggcctg
     3241 ggctcttcgg gaccccgcac gcggcggaag aaaaaggccg acctgggcct ggccgacgac
     3301 cggcccgtcc gccacgtcag cgggcaggac ttgtgccagg ccctgcggct gttggccctc
     3361 gaccagtacg gctatctggc gaagctggtg ctggcgagct ggggaattca ctcgacgggc
     3421 gacttcggcg agatcgtcta taatctcatc aagatcggaa agatgtcgaa gtccgacacg
     3481 gaccggcgga acgacttcga gaacgtcttt gagttcgacc aggccttcgt ccgcgacttc
     3541 gtgattacca aggaggaaga atcgtgtcgc ccctccagtc cctgattgcc agcggcacga
     3601 agctctggct cgattccgtc gatccggatc tcgtccgtag caaccgggcg ctgggcgcga
     3661 ccggggcgac gagcaacccg gtgatcgtca gcgacctgat caagaccggc cgctttgacc
     3721 gcgacctgga gcggctggtc gcggccggca agtcgaacga cgaagtcgcc tggcagctca
     3781 ccgaccagct cgtgcggcag gcgcaggaag tctttctgcc ggtttgcagg gagacgcgcg
     3841 gcaacgacgg ctacgtcagc ttcgagctgg agccgctgtt ggaagatccg cagctccatc
     3901 tgccgctgga ggagcggaca aagcggtacg tcgagctggg caagcaatgg tcggccggcc
     3961 accagaaccg gatgatcaag gtgccggcga caccggcggg catctccgcg ctggagccgc
     4021 tggcggccgc gggcgtgacg ctcaatgtga cgctcgtctt cacgccgcgg caataccacg
     4081 cggcccgcga ggcgatctgg cgcggggccc agaagcgcag cagcctggcc ggcttcaaga
     4141 gcgtctacag catcttcgtc tcgcgcgtgg atatctacac cgagaagcac gtccctcagc
     4201 tgagccccgc cgctcagggc ctggtgggaa tcgtcaacgc caagcaaatc tgggccgaca
     4261 accaggcctg gtggaaggac aagaagctgc cgctggctca ggagatcatc ttcgccagca
     4321 ccggcaccaa gaagcccagc gatccgccct ggaaatacgt cgaggctttt gccggcagcg
     4381 acatccagac caacccgccc gccaccaacg acgccgtcga aaagagcggc cgcaccttta
     4441 cgcggcaagt cgataagccc ccgccgcaac ccgtcctggc cgagatcgcc gccaaggtcg
     4501 atatccaaaa actcgaagac gtgctcatgg acgagggcat ccagaagttt gccgacccgc
     4561 agaaggcgct cctcaagctg atcgccgaga agcgggcggc gctggccaaa tgacgcagca
     4621 caaagagagg gagagggggc gagtgggaga gggggagaca gaagttaagg attccatctc
     4681 cccctctccc cctctcccag tctccccctc gctcgcggac gagccaagga acttctggct
     4741 gctggccgtc tatcaaatcg tcct
//
LOCUS       DASRJB010000187         8258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1745097, whole genome shotgun sequence.
ACCESSION   DASRJB010000187 DASRJB010000000
VERSION     DASRJB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745097"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFB80_05260"
     CDS             <1..274
                     /locus_tag="VFB80_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN33202.1"
                     /translation="DIVVMDNLSSHKIAGVREAIEAVGAELRYLPPYSPDLNPIELAF
                     AKLKKLLRDGAERTADKLWDLCGRLLDEFTEPECRNYFKHCGYRYT"
     gene            complement(357..1610)
                     /locus_tag="VFB80_05265"
     CDS             complement(357..1610)
                     /locus_tag="VFB80_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HZN33203.1"
                     /translation="MKIKLLLLAFAAIAFAAPAPGRSQETIGDRVLRLAGHWADSTSG
                     LCSAPIGPRHAEDISCSDGCCGECNCCCRRLDVFGSVEYLMWWAKGTHLPPLVTTSDP
                     GTLQPDAGVLGLPSTTILFGNEYVGKDMQVGGRVMLGIWLDQEHNVAAGGRFFGLGGD
                     TTQFTATSDGSEILARPFFNAFLGIDDALLIAFPGFAAGSVAATHDTLNIIGTEAFME
                     IMMSRDQCRRLSLVGGYQFMRLDDRLQIVSNHTLLQPGLQFNITDRFKAYNEFHGGEF
                     GLRGQMFRGCWSLDALAMVALGGMRQEVVIEGETVITPPGGGPIATQGGLLAQPSNIG
                     TYERGKFTYIPSLTLNLKYHVNPCLNFFLGYNLIFLGDVALSGDQIDLRVNLNQPVGP
                     PLPAFTFQDRDYWIQGMNFGASWDF"
     gene            2023..3993
                     /locus_tag="VFB80_05270"
     CDS             2023..3993
                     /locus_tag="VFB80_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZN33204.1"
                     /translation="MPSLSVNGIPTGWRAGVLSLVGGPIQRRLAQWGRVLEQIAELEP
                     ALKQETDRELKKRSLSLKYRAKAGEALAKLVPEGYALVREAAVRTIGQRHYDVQMIGG
                     IALFHGCITEMETGEGKTLTATLPLYIHALVGKGAHLATVNDYLADRDANFMRPIYEM
                     LGLSVGVILTPQKSAERRPAYACDITYGTAKEFGFDFLRDRLLLRRMGVTQAGIFGEI
                     MASRMEPGGEQPVQRGAHYVLVDEADSILIDEARTPLIIGSLGDKSIEKITATYQWAA
                     AVVPQFAVDEHYEYDHDDKKVELTGAGRQQVRLLPKPELLSTVGLIDLYQYVERAIKV
                     DRDFHLDRQYVIKDGELVIVDENTGRLAEGRKWRDGIHQAIEAKERIEVTVPTGQAAR
                     ITVQDLFLRYKHLAGMTGTAVPAAREFRKVYKKVVIPVPTNRPAKRTRLPDLVFGTAD
                     EKWLAIVEEARELYQQGRPVLIGTRSIDKSVILSQLLTQAGIEHKVLNAHEIAAEAEI
                     VARAGQPAKVTVSTNMAGRGTDIKLGPGVADAGGLHVICTELHDAQRIDRQLMGRCGR
                     QGDPGTVRQYMSLDDDVLRTGFGPETAEKMIAVGKTNNSSAQGYLNLLKRAQRKVERR
                     HLRDRMTLLHHEKERKKMQAEMGQDPYLDTPD"
     gene            complement(4002..8015)
                     /locus_tag="VFB80_05275"
     CDS             complement(4002..8015)
                     /locus_tag="VFB80_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN33205.1"
                     /translation="MRPAISERRRSFRLQIRSLSVELLERRCLLAALSELTAGQFSQL
                     DIDPREHREGALLVQFRADASAADVAAAEVAGTTLDDGWAIAPGLREVTLAPGTDFLT
                     ALQAYKENPDVLFVEPDFRVSLNLMPNDPLFGSLYGLNNTGQSGGSADADTDAPEAWD
                     VTTGSYAVTVAVIDTGVDYSHPDLFRNIWINQEEIPTAIRNNLVDIDGDTLITFADLN
                     NAVNQGAGRITDLNANGRIDGGDLLNNSSGWENGQDNDADGKIDDLIGWNFVTNTNDP
                     RDDHFHGTHVSGTIGAMGNNGVGVTGVNWNVQIMGLKFLDAAGGGFTSDAIDALNFAV
                     ANGARISNNSWGGDGFSAAFQTALQNAAAAGHIFVAAAGNNSSNNDFSAFYPANFDVE
                     NVVSVAASDRNDQFAWFSNYGASTVDLAAPGLDILSTFPTYMTGEMSSGGFSTNYETI
                     SGTSMATPHVAGALALIASIHPEWTIDQIIGQLLNTVDVIPELSQTITGGRLNAAAAV
                     GNVPPDTTAPRVVSSDPTGGVTGAVSQVRLRFSEAIEAGEFDATDVGSFTGPGGPLSV
                     SGVIPVPGSNRQFDVTFATQTAEGDYELVIGPDIHDLAGNAMAAPYTASFSIVGTLVI
                     DSSDVPAPIFDFTAVGSYLTVDQDVSIADLNVRLDITHTYDADLAIYLISPSGNYTFL
                     SYFYGGAGNHFTNTVFDDEAATPIWSGFAPFTGSYQPDTPLATFDGENAAGTWQLWIE
                     DWGFFDEGTLNSWSIEITPSGSSPPPPPPPPPPPPGNDPPYADYDYFTGVEDEGLPIA
                     ASELLANDTDPDGDTLTVVSVQDAYGGSVSLSGDTITFTPEPNFNYYNNGYAGFSYTI
                     DDGHGNSAVGYVTIELTPVNDAPLAVDDSFSTERNQPLTIQPWELEQNDIDPDGDWLT
                     VPAVSSATDGTVAMDEFGTITFTPAPGFLGTAGFDYTLSDGVETATAHVTILVRDVYY
                     FSLTEGGTLTSSDGSSLTFADADIIELTISAGGAYHYAMYFDGSDVGLTTANEDIDAF
                     AILPTGEILISTLGNYSVAGGSQTGNGSNILIFFPSSLGGSTAGDWGLYFQGSSVGLS
                     GAQENIDAIAILSDGSWIVSTTGNVSVPGVSGVDADLLRYDGSWQMYYDGSDVGLTTA
                     DEDVDGLYIREDAGGGLPRLYLSTRGNFAAGAVSGENDDVLRFTPTRLGGNTAGSYSL
                     SLDGSAYGLAGFDLDGIHVGPAPNASLISALGFLASASADRSQPSDKTGEPAPLAIGD
                     LLFPLQPNLQAQPDKLAAPNAPRPPASNSLSAPAVDHLLAALMARRVQPGSEPRHQAK
                     EQATDDLPPLELFPDDE"
BASE COUNT         1483 a         2579 c         2803 g         1393 t
ORIGIN      
        1 cgacatcgtc gtgatggaca atctctccag ccacaaaatt gccggggtcc gcgaagcgat
       61 cgaagccgtc ggggcagagc tgcggtacct gccgccttac tcgcccgatc tgaatcccat
      121 cgagctggcc ttcgccaagc ttaaaaaact cctccgcgac ggcgctgagc gcaccgccga
      181 caagctgtgg gacctgtgcg gccgcctcct cgacgaattc acggaaccgg aatgccgaaa
      241 ctacttcaag cactgcggat accgctacac ctaaaatgga accgcactag cggcttccgc
      301 gggcaccgcg acagtggtcg cggctttgga ggcaggtgga agccgcgccg cgggacttag
      361 aaatcccagc tggcgccgaa attcatgccc tggatccagt agtcgcggtc ctggaacgtg
      421 aaggccggca ggggcgggcc aacgggctgg ttgaggttga cccgcaggtc gatctggtcg
      481 ccggagaggg ccacgtcgcc gaggaagatc aggttgtagc ccaggaaaaa gttcaggcag
      541 ggattgacgt ggtatttgag gttgagggtc agcgacggga tgtaggtgaa cttgccccgc
      601 tcgtaggtgc cgatgttgct gggctgagcg agcaggccgc cttgcgttgc gatcggtccg
      661 cccccgggcg gcgtgatcac cgtctccccc tcgatcacga cttcctgccg catcccgccc
      721 agagcgacca tcgccagggc gtcgagcgac cagcagccgc ggaacatctg gccgcggagg
      781 ccgaactcgc cgccgtgaaa ctcgttgtag gccttgaagc ggtcggtgat gttgaattgc
      841 aagccgggtt ggagcagggt gtggttgctg acgatttgca ggcggtcgtc gaggcgcatg
      901 aactggtagc cgcccacgag gctgaggcgc cggcactggt cgcgagacat catgatttcc
      961 atgaaggcct cggtgccgat gatgttcagc gtgtcgtgcg tggccgcgac gctgccggcg
     1021 gcgaagccgg gaaacgcgat cagcagggcg tcgtcgatgc ccaggaaggc gttgaagaac
     1081 gggcgggcga ggatctcgct gccgtcggac gtggcggtga actgcgtagt gtcgccgccc
     1141 aggccgaaga accggccgcc ggccgccacg ttgtgctcct gatcgagcca gattcccagc
     1201 atcacgcggc cgccgacctg catgtccttg ccgacgtatt cgttgccgaa caggatcgtc
     1261 gtcgagggca gtccgaggac gccggcgtcg ggttgcagcg tgcccggatc gctggtcgtc
     1321 accagcggcg gcaggtgggt ccccttggcc caccacatca gatattcgac gctgccgaag
     1381 acgtcgagcc gccggcagca gcagttgcac tcgccgcagc aaccgtcgct gcaggagatg
     1441 tcctcggcgt gccgcggccc gatgggggcc gagcacaaac cgctggtcga gtcggcccag
     1501 tggcccgcga gccgcagcac gcggtccccg atcgtttctt gcgaccggcc cggcgctggt
     1561 gcagcgaagg cgatcgccgc gaacgccagg agcaacaact tgatcttcat gggccgcgcc
     1621 ttggtccgta ttacaccggc cacacaccgg actgcggtca tacaactgcg ctagtcgtta
     1681 ggattggatc gaccggcaac accggaaaat ccagcaaagc cggcaaaacc ggcaggcttt
     1741 gcctggattg ccgcgcaggc tctcccggcg cgcgactccg gcgtggtcgt gcttcatttt
     1801 cggccgggaa gactgtgccg attgagtaat ttctgccggc tgcagcggct gtgcggcccc
     1861 aagtgccagc gtggcgacgc cagcaaaccc agcgacccct gtggtgcaat cacttatgat
     1921 gatgaagagc ggtgctttgc ggcacgccag atgcgtgcag ccgccagcac tcagttccgc
     1981 ctgccatcct gactcccgac ccctgactcc tgacccccgc ccatgccgtc gctctcggtc
     2041 aacggaatcc ccaccggctg gcgggccggc gtgctgtcgc tggtcggcgg gccgatccag
     2101 cggcggctgg cccagtgggg ccgagtgctg gagcagatcg cggaacttga accggccttg
     2161 aagcaggaaa ccgaccgcga gctgaagaag cgcagcctgt cgctgaagta ccgggccaag
     2221 gcgggtgagg cgctggcgaa gctcgtgccg gagggctacg ccctggttcg cgaggcggcc
     2281 gtccgcacca tcggccagcg gcactacgac gtgcagatga tcggcggcat cgccctcttc
     2341 cacggctgca tcaccgagat ggaaaccggc gagggcaaaa cgctgacggc tacgctgccg
     2401 ctctatattc acgcgctcgt gggcaagggc gcccacctcg cgacggttaa cgactatctg
     2461 gcggatcgcg acgcgaactt tatgcggccg atctacgaga tgctcggcct gtccgtgggt
     2521 gtgattctca cgccgcaaaa gagcgcggag cgccgcccgg cctacgcctg cgacatcacc
     2581 tacggcacgg ccaaagagtt cggcttcgat tttctccgcg accgactgct cttgcggcgg
     2641 atgggcgtga cgcaggccgg cattttcggc gaaatcatgg cctcgcggat ggagccgggc
     2701 ggcgagcagc cggtgcagcg cggggcgcac tatgtgctgg tggacgaggc cgacagcatc
     2761 ctgatcgacg aggctcgcac gccgctgatc atcggctcgc taggggacaa gtcgatcgag
     2821 aaaatcacgg ccacgtatca atgggcggct gccgtcgtgc cgcagttcgc ggtcgacgag
     2881 cactacgaat acgaccacga cgacaagaag gttgaattga ccggggccgg aaggcagcag
     2941 gtgcggctgc tgcccaagcc tgagctgctt tcgaccgtcg gcctgatcga cttgtatcaa
     3001 tacgtcgagc gggcgatcaa ggtcgatcgc gattttcatc tcgaccggca gtacgtgatc
     3061 aaggacggcg agctggtgat cgtcgatgag aacacgggcc gcctggccga gggccgcaaa
     3121 tggcgcgacg gcatacacca ggcgatcgag gccaaggagc ggatcgaggt cacggtgccg
     3181 acggggcagg ccgcgcggat cacggtccag gacctgtttc tccgctacaa gcacctggcg
     3241 ggcatgaccg gcacggcggt gcccgcggcc cgcgagttcc gcaaggtcta taagaaggtc
     3301 gtcatcccgg tgccgacgaa ccggccggcc aagcggacgc ggctgccgga cctggtgttc
     3361 ggcacggccg acgagaagtg gctggcgatc gtcgaggagg cccgcgagct gtaccagcag
     3421 gggcggccgg tgctgatcgg cacgcggtcg atcgacaaat cggtgatcct ctcgcagctg
     3481 ctgacgcagg caggcatcga gcacaaggtg ctcaacgccc acgagatcgc cgccgaggcc
     3541 gagatcgtgg cccgcgccgg gcagccggcg aaggtcacgg tttcgacgaa catggcgggc
     3601 cgcggcacgg acatcaagct cggtccggga gtcgccgacg cgggcggcct gcacgtgatc
     3661 tgcaccgagc tgcacgacgc gcagcgcatc gaccggcagc tgatgggccg ctgcggccgc
     3721 cagggggacc cgggcacagt gcggcagtac atgtcgctgg acgacgacgt gctgcggacg
     3781 ggcttcggcc ccgagaccgc cgagaagatg atcgccgtcg gcaagacgaa caactcgtcg
     3841 gcgcagggct atctcaacct gctcaagcgg gcccagcgga aggtcgagcg ccgccacctt
     3901 cgcgaccgca tgacgctgct gcaccacgag aaggagcgga agaagatgca agccgagatg
     3961 gggcaggacc cgtatctcga cacgccggat tagcaggcga gctattcatc gtccgggaag
     4021 agctcgagcg gcggcaaatc gtcggtggct tgctccttgg cctgatggcg cggttcgctg
     4081 ccgggctgga cgcggcgcgc catcagcgcg gcgagtagat gatcgaccgc gggggcgctg
     4141 agggaatttg aggcgggcgg ccgcggcgcg tttggcgcgg cgagtttgtc cggctgtgct
     4201 tgcaggtttg gttgcaacgg aaagagcagg tcgccaatgg cgagcggagc cggttcacca
     4261 gttttgtccg agggctggct gcggtcggcg ctagcgctgg ccaggaagcc gagcgcgcta
     4321 atgagtgagg cattcggagc ggggccaacg tgaattccgt cgaggtcgaa tccggccagg
     4381 ccgtaggcgc tgccgtcgag actgaggctg taggagccgg cggtgttgcc acccaggcga
     4441 gtcggcgtga agcggagcac gtcgtcgttc tcgccgctga cagcaccggc cgcaaagttg
     4501 ccgcgcgtcg agaggtacag ccgcggcagc ccgccgccgg cgtcctcgcg aatgtacagc
     4561 ccatccacgt cttcgtcggc cgtggtcagg ccgacgtcgc tgccgtcgta gtacatctgc
     4621 caggagccgt cgtagcgcag caagtcggca tcgacgccgc taacgcccgg aacggagaca
     4681 ttgccggtcg tcgagacaat ccaggagccg tccgacagga tggcgatggc gtcgatattc
     4741 tcctgagcgc cggaaagtcc cacgctgctg ccctggaaat aaagtcccca gtcgcccgcg
     4801 gtactgccgc cgagcgagga gggaaagaag atcaggatgt tgctgccgtt gcccgtctga
     4861 ctgccgccgg cgacgctgta gttgccgagc gttgaaatca ggatctctcc ggtgggcagg
     4921 atggcgaagg cgtcgatatc ctcgttggca gtcgtcaggc cgacgtcgct gccgtcgaag
     4981 tacatcgcat agtggtacgc gcctccggcg gagatcgtca gctcgatgat gtcggcgtcg
     5041 gcgaacgtca aactcgagcc gtcgctgctg gtcagcgtgc cgccctcggt cagcgagaag
     5101 taatagacgt cccggacgag gatcgtcacg tgcgcggtcg ccgtctccac gccgtcggag
     5161 agcgtgtagt cgaagccagc cgttcccagg aagccgggag cgggcgtgaa cgtgatcgtg
     5221 ccgaattcgt ccatcgccac cgtgccgtct gtggctgaac tgacggcggg caccgtcagc
     5281 cagtcgccat ccggatcgat gtcgttctgc tccagctccc acggctggat ggtgagcggc
     5341 tgattccgtt cggtgctgaa cgagtcatcc acggcgagcg gagcgtcgtt cacgggcgtc
     5401 agctcgatcg tgacatagcc gaccgccgag ttgccgtggc cgtcgtcgat cgtgtaactg
     5461 aagccggcgt agccgttgtt gtagtagttg aagttgggct cgggcgtgaa ggtgatcgtg
     5521 tcgccgctga ggctgaccga gccgccgtag gcgtcttgca cggagacgac cgtcagcgtg
     5581 tcgccatcgg gatcggtgtc gttcgccagc aactcggatg ccgcaatcgg gagcccctca
     5641 tcttccacgc ccgtgaaata gtcatagtcc gcgtacggcg gatcgttgcc gggcggcgga
     5701 ggaggcggag gcggtggcgg tggcggcgac gagccggacg gcgtgatctc gatcgaccag
     5761 ctgttgagcg tgccttcgtc gaagaagccc cagtcctcaa tccagagctg ccaggtgccg
     5821 gccgcgtttt cgccgtcgaa agtcgccagc ggcgtatccg gctgatagga gccggtgaac
     5881 ggtgcgaacc cggaccagat gggcgtggcc gcctcgtcgt cgaacaccgt attagtgaag
     5941 tggttgcccg ccccgccata gaagtacgac aggaacgtgt agttgccgct gggcgagatc
     6001 aagtagatcg ccaggtcggc gtcgtaggtg tgcgtgatgt cgagccgcac gttgaggtcg
     6061 gcgatcgaga catcctgatc aaccgtcaga taggagccga cggccgtaaa gtcaaagatc
     6121 ggcgcgggca cgtcgctcga atcgatcacg agcgtgccga cgatcgagaa gctcgccgtg
     6181 taaggggccg ccatcgcgtt gccggccagg tcgtggatgt ccggcccgat caccaactca
     6241 taatctccct cggccgtctg cgtcgcgaac gtgacgtcaa actgccggtt gctgcccggc
     6301 acgggaatca cgccactgac cgacagcgga ccgcccgggc cagtgaagct gccgacgtcg
     6361 gtagcgtcga actcaccggc ctcgatcgcc tcgctgaatc gcagccgcac ctggctgacc
     6421 gcgccggtca ctccgccggt cgggtcgctg gaaacaacgc gtggagcagt cgtgtcgggc
     6481 ggaacgttgc cgacggctgc ggcggcgttc agtcggccgc cggtgatcgt ctgcgacaat
     6541 tcagggatga cgtccacggt attgagcagc tggccgatga tctggtcgat cgtccattcc
     6601 ggatggatcg acgcgatcag ggccaaggcg cccgccacgt gcggcgtcgc catcgacgtg
     6661 ccgctgatcg tctcgtagtt cgtcgagaag ccgccgctgg acatctcgcc ggtcatgtag
     6721 gttggaaacg tgctgaggat gtccagcccc ggcgcggcca gatcgaccgt gctcgcgccg
     6781 tagttcgaga accaggcgaa ctggtcgttg cggtcgctcg cggcgaccga gacgacgttt
     6841 tccacgtcga agttcgcggg ataaaacgcg gagaaatcgt tgttgctgct gttgttgccc
     6901 gccgcggcga cgaagatgtg cccggcggcg gccgcgtttt gcagcgcggt ctgaaaggcg
     6961 gccgagaagc cgtcgccgcc ccagctgtta ttcgaaatcc gggccccgtt ggccacggca
     7021 aaattgaggg catcgatggc gtcgctcgtg aaaccgccgc ccgccgcgtc caggaacttg
     7081 agtcccatga tctggacgtt ccaattgacg cccgtcacgc cgacgccgtt gttgcccatc
     7141 gcgccgatcg ttcccgagac atgcgtgcca tgaaaatggt cgtcccgggg gtcgttcgta
     7201 ttggtgacga aattccagcc gatgagatcg tcgattttgc cgtcggcgtc gttatcctgc
     7261 ccgttctccc agccgctgga gttgttgagc aggtcgccgc cgtcgatgcg gccattggca
     7321 ttgaggtccg taatcctccc cgccccttga ttcaccgcgt tgttgagatc ggcgaacgtg
     7381 atcagcgtat cgccgtcgat atccaccagg ttgttgcgaa tcgcggtggg gatctcttcc
     7441 tgattgatcc agatatttcg aaagaggtcg ggatgtgaat aatcgacgcc cgtatcgatg
     7501 acggcgacgg tcaccgcata ggaccccgtg gtcacgtccc acgcctcggg cgcgtcggtg
     7561 tcggcgtcgg cactgccgcc cgactgaccc gtattgttca ggccatacag cgagccgaac
     7621 aacggatcgt tgggcatcag gttcagcgag acgcggaagt ccggctcgac gaacaggacg
     7681 tccggatttt ccttgtacgc ctggagggcc gtgaggaaat cggttcccgg cgcgagcgtc
     7741 acctcgcgca gccccggcgc gatcgcccag ccatcgtcga gcgtcgtccc cgcgacctct
     7801 gcggccgcga cgtccgcggc gctggcgtcc gcgcggaact gcaccagcag cgcaccctcc
     7861 cgatgctccc gcgggtcgat gtccagttgg gaaaactggc ccgccgtcaa ttcgctcagg
     7921 gcggccagca agcagcgccg ctccagcagc tccacgctca ggctgcggat ctgcagccga
     7981 aaactccgcc tccgctccga aatcgcaggg cgcatcgtgg cctccaagag aagaagagcg
     8041 ccaatcgagc acaaaaaccg accgaggcag catccctaag cgaagaatcg cagcggcgat
     8101 tgtgatgctc cgacgccaat gtgtcaatca atcctcgctg caatatgaag aaaagcagct
     8161 tatcgcttcc gcaccggatt ccgcggttag tcgtgtcggt cccgccgcgc tgatggcgaa
     8221 ttgattagca gcaggtttcg agcgcacgac ggtggccg
//
LOCUS       DASRJB010000188         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4003250, whole genome shotgun sequence.
ACCESSION   DASRJB010000188 DASRJB010000000
VERSION     DASRJB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003250"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1858)
                     /locus_tag="VFB80_05280"
     CDS             complement(200..1858)
                     /locus_tag="VFB80_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN33206.1"
                     /translation="MRWGATSISRPDMFMALALLASLVLAADGPAGQRALEQHALEQH
                     ALTHEGNAERGRELFVKDTLTRCAACHKVSGEGGDAGPDLTHIGGKLGRPHLVESLLE
                     PSRQIVEGYRTTIIRTADGVIHTGVVKEQTDTQLTYVDVGGQRHALLAADIEERQDSP
                     LSLMPQNLAEALKAEQFTDLVAYLETLRSGGKPTPGAGITGPIRLPAGFEVRTIATGL
                     SGCTALEAAPDGRLFVCEQTGTLRVVKHGRLLAAPFVQLPVDSSWERGLIGVTVDPHF
                     PQSPYVYLCYVAKEPFPHHRISRFTARGDVAAAGSERVLLAGDDQRKLGGKVPAGHQG
                     GALHFGKDGKLYIGIGEQTAETPAQRLDTFQGKILRINPDGTIPDDNPLVRTAEGKYR
                     AIWAVGLRNPFTFAVRPTTGELFINDVGGKFEEINRGVAGANYGWPAVEHGPTSEAAF
                     RGPGFYYPQASIAGGDFCPPSSIWPPTWHGRYFFGDFVHGWIKSLDPDHPDQAETFAT
                     GLSRPVDLRFGPNGSLYVLLRNAWVIDDKFQAGTSALLEIRPHE"
     gene            1939..>3628
                     /locus_tag="VFB80_05285"
     CDS             1939..>3628
                     /locus_tag="VFB80_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33207.1"
                     /translation="MSMALGRIAAACLLLAAGSHAASAQQVERAAVDLYGDPLPKGAV
                     ARLGTTRFRRQGDWGKGLALTPDGAMLLSASENHSIYCWDAATGRLIREIDVSPMHIR
                     GFALSRDGRQISVAGFWYSEDRTRSERQVRILDATSGQTLQTLNRDDTSLDRHEMAFT
                     PDGKLLLSLGTAGIFRVEEPASGIEILQEKFPRDVNPYFALSPDGSTIAVATGANTRK
                     FYLWKWQSGEQPREIAITGSDRSYDQLAFSPDGTLLAASLDFGDHAIHLWDVAGGKIV
                     RRLALSDPRTHVRGSIVFTPDGQTLAMPGYRNDSSEPVAVHFWNPHSGEYQGRMDVSG
                     SRLAMSADGRLLAVGGDAHVRVWKWPSREEIAPIDDAHQGGIGRVATTKSGEVVTASD
                     DGTIRVWNPATQRQRLKLTHGKYLRDIAVSPDGSLLVSGAFDDTVRLWDLASGREVFR
                     LPGHGRMNGARSVAFAADGRSFGSFGEDYNLRLWDVRTGKALVEHRVQPTGVKIPDPE
                     ERDANEFFHADQAQFSPDASLFLFTVGKHFVFRTASGQELQAIENRGSHVSALAV"
BASE COUNT          600 a         1179 c         1234 g          615 t
ORIGIN      
        1 ctaaccgatg gcgctggcca tgatggcttc ggggcttgat agggatttgt gtccacgcgc
       61 gtcctatgca gactcagccc aagcggtccg aggacttgtt gggcaggcgt gacttgctgg
      121 gcaaagctac gaagctttga agattataga catatgtaaa caagagtcaa tatccagggg
      181 actacagaga cctcccgggc tattcgtgcg gcctgatttc caataaggcg ctggtgccgg
      241 cctggaactt gtcgtcgatg acccaggcgt tgcggaggag gacgtagagc gatccattcg
      301 ggccgaagcg gagatcgacg ggacgggaca ggcccgtggc gaatgtctcg gcctggtcgg
      361 gatgatcggg gtcgagcgat ttgatccagc cgtggacgaa gtcgccgaag aaatagcggc
      421 cgtgccaggt cggcggccag atagatgatg gcgggcaaaa gtcgccgccg gcgatcgaag
      481 cctgcggata gtagaacccc ggtccgcgga atgccgcttc gctcgtgggt ccatgttcaa
      541 cggcgggcca gccgtaattg gccccggcca cgccgcggtt gatttcctcg aatttgccgc
      601 cgacgtcgtt gatgaacaat tcgccggtcg tcggccgcac ggcaaaggtg aacggattgc
      661 gcagccccac agcccagatc gcccggtact tgccctcggc ggtgcggacc agcgggttgt
      721 catccgggat cgttccgtcg gggttgatcc gcaggatctt gccctgaaac gtgtcgagcc
      781 gctgggccgg cgtttcggcg gtctgctcgc cgatgccgat gtagagcttt ccgtccttgc
      841 cgaagtgcag cgcaccgccc tgatggcccg ccggcacctt gccgccgagc ttgcgttgat
      901 cgtcgccggc gagcagcacc cgctcgctgc cggcggccgc gacgtcgccg cgagcggtga
      961 atcggctgat gcggtgatgc ggaaacggct ccttggcgac gtagcacaga tagacgtaag
     1021 gcgactgggg aaaatgggga tcgaccgtca ctccgatcag gccgcgctcc cacgaggaat
     1081 cgaccggcag ctgcacgaaa ggagcagcga gaagccggcc gtgcttcacg acccgcagcg
     1141 tcccggtctg ttcgcagacg aacaggcggc cgtccggcgc ggcctccagg gcggtgcagc
     1201 cgctcaggcc agtggcgatg gtgcggactt caaatcccgc ggggagccgg atgggccccg
     1261 tgattcccgc gccgggcgtg ggcttgccgc cggaccgcag cgtttcgaga taggcgacga
     1321 ggtccgtgaa ctgctccgct ttcagtgcct cggccaggtt ctgcggcatc agcgagagcg
     1381 ggctgtcttg gcgctcctcg atgtcggccg ctagcagcgc gtgccgctga ccgccgacat
     1441 ccacgtacgt cagctgcgtg tcggtttgct ccttcacgac gccggtgtga atcacgccgt
     1501 cggccgtgcg gatgatggtg gtgcgatagc cctcaacgat ttgccgcgac ggctccagga
     1561 gtgactcgac caggtgcggc cggccgagct tgccgccgat atgcgtgagg tccgggccgg
     1621 cgtcgccgcc ttcgccgctg accttgtggc aggcggcgca ccgcgtcagc gtgtctttaa
     1681 caaacagctc gcgcccgcgc tcggcgttgc cctcgtgcgt cagcgcgtgc tgctccagcg
     1741 cgtgctgctc cagcgcgcgc tgccccgccg gcccgtcggc cgccagcacg agcgatgcca
     1801 agagcgccag cgccatgaac atgtcaggcc gtgagatcga ggttgcgccc caccgcatag
     1861 atggtggcat cgcacgaggc aatcggctag cctgcccact ggagtttcgc acatcctctc
     1921 ttctgtgcga ggttcgcgat gtccatggct ctgggacgaa tcgccgcggc gtgcttgctg
     1981 ctggcggcag gcagtcacgc agcgtcagct caacaggtgg agcgagcggc cgtcgatctc
     2041 tacggcgatc cgctgcccaa gggcgcggta gcccggctgg gcacgacccg ctttcgccgc
     2101 caaggcgatt ggggcaaggg gctcgcgctc acgcccgacg gggcgatgct gctgtccgcc
     2161 tccgaaaatc attcgattta ctgctgggac gctgccaccg gccgcttgat ccgcgagatc
     2221 gacgtcagcc cgatgcacat tcgcggcttc gccctctcgc gcgacggccg gcagatctct
     2281 gtggccgggt tctggtatag cgaggaccgg acgcggagcg agcgacaggt gcgaatcctg
     2341 gacgcgacga gcggccagac cctccaaacg ctgaatcgcg acgacaccag cctggaccgc
     2401 cacgagatgg ccttcacgcc cgacggcaag ctgctcctct cgctgggcac cgccggcatc
     2461 ttccgcgtcg aagagccagc cagcggcatc gaaatcctcc aggaaaaatt tccccgcgac
     2521 gtgaatccct attttgctct ttcgccggac ggttcgacga tcgccgtggc gacgggggcc
     2581 aacacccgca agttctacct ctggaaatgg caaagcggcg agcagccgcg ggaaatcgcg
     2641 atcaccggca gcgaccggag ctacgaccag ctggccttct cgccggacgg cacgctgctc
     2701 gcggccagcc tggattttgg cgatcatgcg atccacctct gggacgtcgc cggcggaaag
     2761 atcgtgcggc ggctggcact gagcgatccc cggacccatg tccgcggcag catcgtcttt
     2821 acgcccgacg gccagacgct ggcgatgccc ggctatcgca acgacagcag cgagcccgtc
     2881 gccgtccact tctggaatcc gcacagcggc gagtaccaag ggcggatgga cgtgtcgggc
     2941 agccggctgg ccatgtcggc cgacgggcgc ctgctggcgg tgggcggcga cgctcatgtg
     3001 cgggtctgga agtggccctc gcgcgaagag atcgccccca tcgatgacgc ccaccagggc
     3061 ggcatcggcc gcgtagcgac gaccaaaagc ggcgaggtgg tgacggccag cgacgacggg
     3121 acgatccgcg tttggaatcc ggcgacgcag cgccagcggc tgaagctcac gcacggcaag
     3181 tatctgcgcg acatcgcggt ttcgcccgac ggctcgctgc tcgtctccgg ggccttcgac
     3241 gacacggtgc ggctgtggga cctggccagc ggccgcgagg tctttcgcct gccggggcac
     3301 gggcggatga acggcgcgcg gtccgtcgcc ttcgccgccg acggccgcag cttcggctcg
     3361 ttcggcgagg attacaacct gcggctgtgg gacgtgcgga cgggcaaggc cctggtcgag
     3421 cacagggttc agccgaccgg cgtcaaaatt ccagacccgg aagagcgcga tgcgaatgag
     3481 ttcttccacg ccgatcaggc tcagttctcg cccgatgctt cgctgttcct gttcacggtg
     3541 ggcaagcatt ttgtgtttcg caccgcctcg ggccaggaat tgcaggcgat cgagaaccgc
     3601 ggctcgcacg tctccgccct ggccgttt
//
LOCUS       DASRJB010000189         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1179948, whole genome shotgun sequence.
ACCESSION   DASRJB010000189 DASRJB010000000
VERSION     DASRJB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179948"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFB80_05290"
     CDS             <1..243
                     /locus_tag="VFB80_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33208.1"
                     /translation="GWLGTSLAVVGALVSALYLANLTAGAFEFPPDVFPGVGNLDEFA
                     FSLLLVYCLQKLGINLLPFMKPGALPLGEARRADRK"
     gene            complement(442..2796)
                     /locus_tag="VFB80_05295"
     CDS             complement(442..2796)
                     /locus_tag="VFB80_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HZN33209.1"
                     /translation="MAFINLSLLAGGLCVAIPVVLHLIMRQRPKQFIFPALRFIQQRR
                     IANQRRLQLRHWLLLALRCGAIGLFALALARPSVTSGTLSSWIAAGFLGAMGLVAGVS
                     SGVGFARNLSRLVAGTMAAVAAILLVMAIYLGGRAAAGRPPVLGDQEAPVAAVLVIDT
                     SPRMQYRSENRTRLAAAQEMAHWLIRQLPGESELAVVDSRSGSGAFAVDRAAAEKAIE
                     RLRPVGTPRPLAEVLEHSVQLVRQKPQPRKEIYVFTDLTQAAWKTTAADGLKKLLADH
                     PGVLLYVLDVGAENPRNFALGPLTLSADVLPAGADLKLETSASASGVSGERSVELQIE
                     TPDPTLPVIRDGQPVFPKSQRRGVSSVKLEPGGQQQVQFTVSGLPAGVHQGLVRLVGE
                     DGLALDDVRYFAVEVQPAWPVLVVAPADVSPRLFIEMLAPLEQRESGRARFKCDAIDQ
                     ARLADAELADYRAVVLLDPKPLTPDLWKKLAEFCQTGGGLAVFLGHNADPPASFQDPA
                     AVAVLGGKLTRVTRTGGDIYLAPRSYDHPALAAFRPVANNVPWDLFPVFYHWNLDNLA
                     ATARSIVPFGDGKPALVENRLGRGSALVMTTPVSDLPRPVDHPLWNDLAAGENIWPCV
                     LLVHESMTYLVRSGQTRLNLLAGETAVLPNDPAEFPERYQLFTPLEQPQDVLARDRRV
                     TVRFTDNPGAYRLRGQKGGPLVRGFAVNLAGDTSDLSRLPREKLDELLGAKRFQLARS
                     QDELNRAVGNDRIGSEFYPLLIALVAVALGLEHVLANRFYRKDD"
BASE COUNT          465 a         1027 c          980 g          458 t
ORIGIN      
        1 ggttggctgg gtacatcgct ggcggttgtt ggggcgctag tgtcggcctt gtacctcgca
       61 aacctgacgg ctggggcgtt cgaattcccg cctgatgtct ttcccggcgt gggcaatctc
      121 gacgagttcg ccttctcgct gctgctcgtc tattgcttgc agaagctcgg tatcaacctg
      181 ctgccgttca tgaaaccggg tgcgctgcca ctgggtgagg cccggagggc cgacaggaag
      241 tagccccgac cgtcaggtcg gggataatta cgtttgtcgt gacgaccgag aggcccggag
      301 ggccggcacg cgccccgcga atgcacggca tcatgtgccg gccctccggg cctccctccg
      361 actttggcgc acagaaaccc cgacctcacg gtcggggcta ttgcctgtcg gccctccggg
      421 cctgaggagc gccattcggc ctcaatcgtc cttccgataa aaccggttcg ccaggacgtg
      481 ttccaggccg agcgcgacgg cgacgagcgc gatcaggagg ggatagaact cgctgccgat
      541 ccggtcgttg ccgacggcgc ggttcagctc gtcctggctg cgagcgagct gaaagcgctt
      601 ggccccgagc agctcgtcga gcttttcccg cggcagccgc gagaggtcgc tcgtgtcacc
      661 cgccaggttc accgcaaagc cgcggaccag cggcccgccc ttctggccgc ggaggcgata
      721 cgcccccgga ttgtccgtga accgcaccgt cacgcggcga tcgcgggcca gcacatcctg
      781 cggctgctcc agcggcgtga acagctgata gcgctccggg aactccgccg ggtcgttggg
      841 cagcaccgcc gtttcgccgg ccagcaggtt cagccgcgtc tgcccgctgc ggacgagata
      901 cgtcatcgac tcgtggacga gcagcacgca gggccagatg ttctcaccgg cagccaggtc
      961 gttccagagc ggatgatcga cgggccgcgg caagtcggaa accggcgtcg tcatgaccag
     1021 cgcactgccg cgcccaaggc ggttctcgac cagtgccggc ttgccgtcgc cgaacggcac
     1081 gatcgagcgg gcggtcgccg ccaggttgtc gaggttccag tggtaaaaga ccggaaacaa
     1141 gtcccagggc acgttgttag ccaccggccg gaacgccgcg agggccggat ggtcgtacga
     1201 ccgcggggcg agataaatgt cgccgccggt gcgggtgacg cgcgtcagct tgcctccgag
     1261 caccgccacc gccgccgggt cctgaaacga cgccggcggg tcggcgttat gacccaggaa
     1321 tacggccagc ccgccgcccg tctggcagaa ttctgcgagc ttcttccaca agtccggcgt
     1381 cagcggcttc ggatcgagca gcaccacggc ccggtaatcg gccagctcgg cgtcggccag
     1441 gcgggcctgg tcgatcgcgt cacacttgaa ccgcgcacgc cctgattctc gctgctccag
     1501 cggcgcgagc atttcgatga acagccgggg cgaaacgtcc gcgggggcca cgaccagcac
     1561 cggccacgcc ggctgcacct cgaccgcaaa ataccgcaca tcgtcgagcg ccaggccgtc
     1621 ctcgccgacc agccgcacga gtccctggtg aacgcccgcg ggcaggccgc tgacggtgaa
     1681 ttgcacctgc tgctgaccgc ccggctcgag cttcacgctc gatacgccgc ggcgctggct
     1741 cttgggaaac accggctggc cgtcgcggat cactggcagc gttgggtcgg gcgtttcgat
     1801 ttgcagctcg acgctccgct cgccgctgac gccgctcgcg ctggcgctcg tctccagctt
     1861 caggtccgcc ccggccggca gcacgtcggc cgaaagcgtc agcggcccga gcgcgaagtt
     1921 ccgcgggttt tccgccccga cgtcgagcac gtacagcagc acgccgggat gatccgccag
     1981 cagcttcttc aggccatccg ccgcggtggt cttccaggcc gcctgcgtga ggtcggtgaa
     2041 gacgtagatc tcctttcgcg gctgcggctt ttggcggacg agttgcaccg agtgctctaa
     2101 aacctcggcc agcggccgcg gcgtgcccac cggacgcagc cgctcgatcg ccttttcggc
     2161 ggcggcccga tcgacggcga acgcgccgct gcccgagcgg ctgtccacga cggccagctc
     2221 gctttcgccg ggcagctggc ggatgagcca gtgggccatc tcctgggcgg ccgcgaggcg
     2281 ggtgcggttc tcgctgcgat actgcatccg cggcgaggtg tcgatgacga gcacggcggc
     2341 gaccggcgct tcctggtcgc ccagcaccgg cggccggccc gccgcggctc gcccgccgag
     2401 atagatcgcc atcacgagca ggatcgccgc caccgcggcc atcgtgccgg cgacgagtcg
     2461 cgacaagttg cgggcaaatc ccacgccgct ggagactccc gccaccaggc ccatcgcgcc
     2521 gaggaagccg gctgcgatcc agctcgagag cgtgccgctg gtcacgctcg gccgggccag
     2581 cgccagcgca aacaggccga tcgccccgca ccgcagggcg agcagcagcc aatgtcgcag
     2641 ctgcaatcgc cgctggttcg caatccgccg ctgctggatg aaccgcaggg ccggaaagat
     2701 gaactgcttg ggccgctgcc gcatgatcag gtgcagcacg accgggatgg cgacgcacag
     2761 cccccccgct agcagcgaca gattgatgaa cgccatgaac gcttcgactt tgttccgcga
     2821 taccgacctc catattagcc ggacattcgc ctggacgctg cggtacggcg ttgatactgt
     2881 ccgcaccgat tgtgcaggtc cccatctgcc ggaagggacc cggagggagc
//
LOCUS       DASRJB010000190         8491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_616340, whole genome shotgun sequence.
ACCESSION   DASRJB010000190 DASRJB010000000
VERSION     DASRJB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616340"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFB80_05300"
     CDS             <1..955
                     /locus_tag="VFB80_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN33210.1"
                     /translation="CLGGDGAMFDIGFQSLSRMFASGMNIKVFVLDTQVYSNTGGQAS
                     TSSYTGQNTKMSVHGKAIAGKQERRKEIAQIAMMHPRTFVAQTTCAHINHFYKSVIDA
                     MEFDGPAIVCCYTTCQPEHGVADNMATDQARLAVDTRAFPLLIYDPRKGDTIKERLSL
                     QGNPAQTEDWWTNPKTGQQVDFIDFARSEGRFGKHFDKAGNPSPTLLYAKQDRLENWH
                     VLQDLAGLRGKPAAKAPAKPAAEKPEPKAAAPAKANGNGKQNGDFAVGTRIKYNDGAG
                     WVSGVVASTSPAVLKFDDNTIIETSYDVLVAGAAEGIIVRQ"
     gene            1045..1335
                     /locus_tag="VFB80_05305"
     CDS             1045..1335
                     /locus_tag="VFB80_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33211.1"
                     /translation="MHESHGDLLIGGERIKHVHCELEEEQPLTDSKEWILAGHLHLTV
                     EQSQLLETDRPYRLQLEDGRAGQVVVSSISCDGKPDESVVIFQPKKTPEKTR"
     gene            1353..2024
                     /locus_tag="VFB80_05310"
     CDS             1353..2024
                     /locus_tag="VFB80_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33212.1"
                     /translation="MRAIRLLFARSSASKRARKLFVPNLSERERFRRLQQLSDGPYVA
                     LTILTARRFLRDFPEHGPAWLLLGIALVEAARYEEAEQALAKSLNFVPQSRRQLPLWH
                     LGHLFKGSGDYDRAAEYYREAIAACPEDATGYIFLGGVLARGGRLHEAEQAHRAATQC
                     VEGCIDEAYLNLGNVLRALERFEEAAECYREAIRLDPEYTVAKKALRDCTRCIPIKSL
                     RRKQR"
     gene            complement(2025..2267)
                     /locus_tag="VFB80_05315"
     CDS             complement(2025..2267)
                     /locus_tag="VFB80_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN33213.1"
                     /translation="MRTVEHLFETTGLTCDDVAQRAGLPVERVAAIAEGRWTPSPDER
                     ARIAAAFGVPVEEISWGHTMNPRNIRYGRFGLKENF"
     gene            complement(2354..2596)
                     /locus_tag="VFB80_05320"
     CDS             complement(2354..2596)
                     /locus_tag="VFB80_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33214.1"
                     /translation="MSFKYIRLLLMGPLFIVLGVMLMIGSAAIAAPDGDAAAAQAAVS
                     NWRAWAAIAGGIGLSVWALIDIIRTPREKEGKPENE"
     gene            complement(2609..4009)
                     /gene="thrC"
                     /locus_tag="VFB80_05325"
     CDS             complement(2609..4009)
                     /gene="thrC"
                     /locus_tag="VFB80_05325"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZN33215.1"
                     /translation="MALADFAFQRCISPACGQTCGIEEVRVACAKCGDLLDVAYDWSK
                     ARPPKSLRFFEEMWSRRHEPLRFSGIYRFHELLPFATPDSVVTVGEGQTLLQQADAVS
                     QYVGLKPGRLYLQYEGLNPSGSFKDNGMSAAFTHARAIGAKRAACASTGNTSASLAMY
                     CAATRLMKAVIFIGSGKISYGKLSQALDYGALTIQIAGDFDDAMARVQEVSRDLGLYL
                     VNSVNPFRLEGQKTIMFRVLEALRWEVPDWVVVPGGNLGNSSAFGKAFVELLQLGLIE
                     RLPRLAVINAAGADTLFELYERRGLRWNGGAADMGIVNHYYRELDQLGRKASTIASAI
                     EINRPVNLRKCLRALEDLQGVVRQVSDQEMLDAKAKIAAGGFGCEPASAASVAGARLL
                     RDQGVIGADERVVCILTGHQLKDPTATVAYHTTDQEQFNKVLGSRGVKRASFANRAVA
                     VPNDLGEIVKAIQLYS"
     gene            4323..5084
                     /locus_tag="VFB80_05330"
     CDS             4323..5084
                     /locus_tag="VFB80_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33216.1"
                     /translation="MNFYRSLVPAGCLTLAAALGCDFSGQYEQRFQEALQSSAARAAF
                     DQQLFPTETPVTDPSRKETGVKLRIPILFKDSKSLPATDPRAEPPFLKLPGLHYAMER
                     ELDDGTNKNFLPTYVYFAAVPKIEQKVEQKADALQAALAQQLAAAFPGAAWADVQVPT
                     PEGKPITLKRIRVEGQQDFYNIQAKKTDRVDGRFDLYFINSRDHHVLLGWRTPKPQGT
                     RYFYAASDAAMGTVTSNAGTAPAGGSPDGGKKSGG"
     gene            5225..7114
                     /locus_tag="VFB80_05335"
     CDS             5225..7114
                     /locus_tag="VFB80_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33217.1"
                     /translation="MRRALACVMVAGCLFLSGCGGGGSSTPSAGAGTGGGGPPAPPAP
                     PGAPAPGAPPAAPGGSGDAAAAPMPESGSPDGGQPGGAPQPGSPDGGLAGGGRGGFPG
                     PGGSPDGGAAGPGFGGPGPGFGGFPGGSPDGGGGLPGGIPDMSAFRLPVVKSLRDQAI
                     DAFRANNETLGQQLLLTHFAVVPSAGAELAEKMAWIPVLRRPALGPRIGVVAFYDKPP
                     RDFKDSPMPIGSSELAAVVAASQQQQGGDEGGRRRNNQPRPNAGGEQGPALAGGPGIP
                     GAGGDGAGARSGELAFHTGEFGTKLVEAIAARISAGEYGAVYKEISEGMSRPATPDPN
                     NPGDPNAAGAGAPGAPGAFGGDGGPGLGRGRVPGGLAGAAPGGEGAGGEGAAVAGSAQ
                     PLGLCIVWLGKVTSKEEAARLAQEAKVDILATYEIDVKLARNSNFVNNTTLLRITPTK
                     KGEPIFASKALNNLQVVNQKQATENPVDKEITRAMEALDKVCKAAPLPALTAEQVKGQ
                     LARLVAEKPADPLPVIVEARYYVAKGLLPEADMTAAAIGLMGEAEFARLIVSAPEAKG
                     QIVGAALSLPGVVNLLHGLNSAAGAAGATARRPNPQPGAAPGEAPPPRGGLRGLLPFG
                     SGSGR"
     gene            7168..7428
                     /locus_tag="VFB80_05340"
     CDS             7168..7428
                     /locus_tag="VFB80_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33218.1"
                     /translation="MAEYTKYQQKVIRNYYDHREGIALQRVQELVTELYLATGKKRDK
                     HWDSLALHLGKLGVKPDVIEHLRSEDKPELVATLVKKLMDKK"
     gene            7552..>8491
                     /locus_tag="VFB80_05345"
     CDS             7552..>8491
                     /locus_tag="VFB80_05345"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZN33219.1"
                     /translation="MRTAWNFHSAGQLVFGRGSCAQLGTLLSRRKLARVFVVADPRLQ
                     AAGLVEQVSRPLRESGIAAQVFAGGEPEPAVATATRAADVARQFGPDCVLGLGGGSNM
                     DLAKIVAVLLAHGGEPARYFGFDNVPGPVAPLVCVPTTAGTGGEVSHAAVLTDTANHI
                     KVSTLSQHLRPLLAVVDPALTDNCPQQVTADSGIDALTHAIEAYTAIDFDQLAAPDSA
                     PVAYEGRYPLGECLAEKAISLIGQHLVAAVTDGGNRAARDGMALAATLAGLAFSNCAV
                     AVVHALEYPLGGAVHVSHGAGNGLLLPFVMRYNLPVR"
BASE COUNT         1518 a         2823 c         2804 g         1346 t
ORIGIN      
        1 gtgcctgggc ggcgacgggg ccatgttcga catcggcttc cagtcgctca gccgcatgtt
       61 cgccagcggc atgaacatca aggtcttcgt gctcgacacg caggtgtata gcaacaccgg
      121 gggccaggcc tcgacctcga gctacaccgg ccagaacacg aagatgagcg tccacggcaa
      181 ggcgatcgcg ggcaagcagg agcgccgcaa ggaaatcgcc cagatcgcca tgatgcaccc
      241 gcggacgttc gtggcccaga cgacctgcgc gcacatcaac cacttctaca agtcggtgat
      301 cgacgcgatg gagttcgacg gtccggcgat tgtctgctgc tacacgacct gccagcccga
      361 gcacggcgtg gccgacaaca tggccaccga ccaggcccgc ctggcggtcg atacgcgggc
      421 gtttccgctt ttgatctacg atccccggaa gggcgacacg atcaaggagc ggctgagcct
      481 gcaaggcaac cccgcgcaga ctgaagattg gtggaccaat cccaagaccg gccagcaggt
      541 cgatttcatc gacttcgccc gcagcgaagg ccgctttggc aagcacttcg acaaggccgg
      601 caatcccagc ccgacgctgc tctatgccaa gcaggaccgc ctggagaact ggcatgtgct
      661 gcaagacctg gccggtctcc gcggcaagcc tgctgccaag gcgccggcta agcctgctgc
      721 cgaaaagccg gagcccaaag cggccgcacc cgccaaagcc aacggcaacg gcaagcagaa
      781 cggcgacttc gccgtcggca cgcggatcaa gtacaacgac ggcgctggct gggtcagcgg
      841 cgtcgtcgcc tcgaccagcc ccgccgtgct gaagttcgac gacaacacga tcatcgaaac
      901 cagctacgac gtgctcgtag ccggcgcggc ggaggggatc atcgttcggc aataagacaa
      961 ctcgttccca agcatagctt gggaaccagg attgaatcga ctgaggcgtc ccacggcggc
     1021 tgacatcagg cggaggtcag ggcgatgcat gaatctcacg gcgacttgtt gattggcgga
     1081 gagcggatca agcacgtgca ctgcgagctc gaagaggagc agccgctgac ggattccaag
     1141 gagtggatcc tggcggggca cctgcacctg accgtcgagc agtcgcagct gctggaaacc
     1201 gaccggccct atcgcctgca gctcgaagac ggccgcgccg gccaggtcgt cgtcagcagc
     1261 atctcctgcg acggcaagcc ggacgaatcg gtcgtcatat ttcagccgaa gaagacgccg
     1321 gaaaaaactc ggtagttctt ccagcgaacc ttttgcgtgc cattcgactc ttgttcgcaa
     1381 ggtcgagtgc ctcaaagcgc gcgcggaaac tgttcgtgcc aaacctctcc gagcgagaac
     1441 gatttcgtcg cctgcagcaa ctgtcggatg gtccgtatgt ggctctcacc atcctcaccg
     1501 ccaggcgatt cctgcgcgat ttcccggaac acggcccagc ctggctgctg ctgggaattg
     1561 cgctcgtgga agccgcacgc tacgaagaag ccgagcaagc cctcgccaag tccctgaact
     1621 ttgttcccca atcgcgccgc caacttccac tctggcactt ggggcatctc ttcaaaggca
     1681 gcggcgacta cgatcgggcg gctgaatact atcgagaggc gatcgcagct tgtcctgagg
     1741 atgcgaccgg atacatcttc ctgggaggcg tgctcgcccg cggcggccga cttcacgagg
     1801 cagaacaagc ccaccgagct gcgacgcagt gcgtggaagg ctgcatcgac gaggcgtatc
     1861 tgaacctggg aaacgtgctg cgggcgttgg agcggttcga ggaagcagcc gaatgctacc
     1921 gcgaggcgat ccgtctcgat ccagaataca cggtggcaaa gaaggcacta cgcgattgca
     1981 ctcgctgcat ccccatcaaa tctctgcggc gcaagcagcg ataatcagaa attctctttg
     2041 agcccgaacc gcccgtagcg gatgttgcgg gggttcatgg tgtgccccca gctgatctct
     2101 tccaccggca cgccaaaggc cgcggcgatc cgcgcccgtt catccggact cggcgtccat
     2161 cgcccctccg caattgccgc cacgcgctcg accgggagcc cggcccgctg cgccacgtca
     2221 tcgcacgtca aacccgtcgt ctcaaacaaa tgctcgaccg tccgcatcgt tgctctgcag
     2281 gaaactgttg cttggcagaa attggaatcg ggcttcgcag ttcattctac ctggcgaatt
     2341 gcgccgcgag tgctcattcg ttttccggct tcccttcctt ctcgcgcggc gtgcggataa
     2401 tgtcgatcaa ggcccatacg gacagcccga ttccgccggc gattgcggcc caggctcgcc
     2461 agttggacac agcagcttgt gccgcggccg catcgccgtc gggggcggcg atagccgctg
     2521 aacctatcat tagcattaca ccgagcacaa tgaatagcgg ccccattagc agcagacgaa
     2581 tgtatttgaa cgacatagcg cggtctcgtt agctgtagag ctgaatggcc tttacgatct
     2641 cccccaaatc gttcggcacc gccacggccc ggttggcgaa gctggcgcgc ttgacgccgc
     2701 ggctgccgag gactttgttg aactgctcct ggtcggtggt gtggtaggcg acggtggcgg
     2761 tggggtcctt gagctggtgg cccgtcagga tgcagacgac gcgctcgtcg gcaccaatca
     2821 cgccctggtc gcgcaagagc cgcgctccgg cgacgctggc cgcgctggcc ggctcgcacc
     2881 caaatccgcc ggcggcgatc ttggccttgg cgtcgagcat ctcctggtcg ctgacttgcc
     2941 gcacgacgcc ctgcaggtcc tccagggccc gcaggcattt gcggaggttg accggacggt
     3001 tgatctcgat cgcgctggcg atcgtcgagg ctttgcggcc gagctgatcg agctcgcggt
     3061 agtaatggtt gacgatgccc atgtcggccg cgccgccgtt ccaccgcaga ccgcggcgct
     3121 cgtaaagctc gaacagcgtg tcggcccccg ccgcgttgat caccgccagc cgcggcagcc
     3181 gctcgatcag gccgagctgc aagagctcca cgaacgcctt gccgaacgcg ctgctgttgc
     3241 ccaggttgcc gccgggcacg acgacccagt cgggcacttc ccagcggagg gcctccagga
     3301 cgcggaacat gatcgtcttc tgcccttcca gccggaaggg attgacactg ttcacgaggt
     3361 acaggcccag atcgcggctg acctcctgca cccgggccat ggcgtcgtcg aagtcgccgg
     3421 cgatctggat cgtcagcgcg ccgtagtcca gcgcctgcga gagcttgccg tagctgatct
     3481 tgccggagcc gatgaagatc accgccttca tcagccgcgt cgccgcgcag tacatggcca
     3541 gcgacgcgct ggtgttgccc gtggaggcgc aggccgcgcg cttcgcgccg atcgcccggg
     3601 cgtgcgtgaa ggcggccgac atgccgttgt ccttgaacga gcccgacggg ttgaggccct
     3661 cgtattgcaa gtacagccgg ccgggcttga ggccgacgta ctggctgacg gcgtcggcct
     3721 gctggagcag cgtctgccct tcgccgacgg tcaccacgga gtcgggggtc gcaaacggca
     3781 gcagctcgtg gaagcggtag atgccgctga atcgcagcgg ttcatggcgg cgggaccaca
     3841 tctcctcaaa gaaccgcagc gatttcggcg ggcgggcctt gctccagtcg taggccacgt
     3901 cgagcaggtc gccgcacttc gcacaggcga cgcggacctc ctcgatgccg cacgtctggc
     3961 cgcaggcggg gctgatgcag cgctgaaacg caaaatcggc cagggccacg gcgggaagac
     4021 tttggaggca cggaagatga aaacggacgc ggcgggcagc ggcagaatga acagaaggta
     4081 acgaaggaaa cgaagaaagg caggaatcaa ggagccttgg cgtcgcgtca acttctcttc
     4141 tttgccttcg tttccttccg ttccattccc tcgctccatt ttggagttcg cagctaagtc
     4201 taggccgcca aagaaaatgc ggcaagggag accagcagtc agccgccctg tgccgccgat
     4261 tgtgctgccc gcgttttgta ggcatgatgg tgccagttgc gattcactcg atgaggacta
     4321 ccgtgaactt ctaccggtct ctggtgcccg ccggctgttt gactttggcg gccgcgctcg
     4381 gctgcgactt ttccggccag tacgaacagc gattccagga ggcgctccag tcgtcggccg
     4441 cgcgggccgc gttcgaccag cagctctttc cgaccgagac tccggtcacc gaccccagcc
     4501 gcaaggagac gggcgtcaag ttgcggatcc ccatcctgtt caaggacagc aaatcgttgc
     4561 cggccacgga cccgcgggcc gagccgccgt tcctgaagct gccgggcctg cattacgcga
     4621 tggagcgcga gctcgacgac ggcacgaaca agaacttcct gccgacctat gtctattttg
     4681 cggctgtgcc caagatcgag cagaaggtcg agcaaaaggc tgacgcgttg caggccgcgc
     4741 tggcccagca gctggccgcg gcgtttcccg gcgcggcctg ggccgacgtg caggtcccca
     4801 cgcccgaggg caagccgatc acgctcaagc ggattcgcgt cgaaggccag caggacttct
     4861 acaatatcca ggccaagaag accgatcggg tcgatggccg gtttgacctc tatttcatca
     4921 attcccgcga ccaccacgtc ctgctgggct ggcgcacgcc caagccgcag gggaccaggt
     4981 acttctacgc cgccagcgac gccgcgatgg gtaccgtcac ctcgaacgcc ggaaccgcgc
     5041 ccgccggcgg cagcccggac ggtgggaaga aaagcggcgg ctgattgggg ccgattagcc
     5101 cgctcgcacc gcgagcagac gacctcacgc ggagcgtgag gactacagag tttctcgact
     5161 ttcccccggc ggcgcgtcat aatccgggtc ctggaccgtg cttctctttg cgagggtctg
     5221 caaaatgcgt cgcgctcttg cctgcgtcat ggtggccggc tgcttgttcc tcagcggatg
     5281 cggaggcggc ggcagctcga ctcccagcgc gggcgctggt accggcggcg gtggaccgcc
     5341 cgctccccca gcgcctcccg gcgcacccgc tccaggcgct ccgccagcag cgccgggcgg
     5401 cagcggcgat gccgcggctg ctcccatgcc cgagtcgggc agccccgacg gcggccaacc
     5461 agggggcgcg cctcagcccg gcagtcctga cggcggtctg gcgggcggcg gccgcggcgg
     5521 cttccccggc ccgggcggct cgcccgacgg cggcgcggca ggcccaggtt tcggcggccc
     5581 aggtccaggc ttcggcggat ttcccggcgg cagcccggat ggcggcggcg gtcttcccgg
     5641 cggcattccc gacatgtcgg cctttcgcct gccggtcgtg aaatcgcttc gcgaccaggc
     5701 gatcgacgcc tttcgcgcga ataacgagac cctcggtcag cagctgttgc tgacgcattt
     5761 tgccgtcgtt ccttcggccg gcgccgagct ggccgagaag atggcctgga ttccggtttt
     5821 gcggcggccg gcactcggcc cacggatcgg cgtcgtggcg ttctacgaca agccgccccg
     5881 cgatttcaag gacagcccga tgccgatcgg ctccagcgag ctggcggccg ttgtggctgc
     5941 ctcacagcag cagcaggggg gcgacgaagg cgggcggcgg cgaaacaatc agccgcgtcc
     6001 gaacgcaggc ggcgagcaag gacccgccct cgccggcggc ccgggaattc ccggcgctgg
     6061 cggcgatgga gccggtgccc gctcgggcga gctggcgttc cacaccggcg aattcgggac
     6121 gaaactggtc gaagcaatcg ccgcgcggat ttcagcgggt gagtacgggg ccgtgtacaa
     6181 ggagatcagc gaaggcatgt cccgacctgc gactcccgat ccgaacaatc cgggcgatcc
     6241 gaatgccgcc ggcgcgggtg cgcctggcgc gccgggcgcg tttggcggcg atggcggtcc
     6301 cggtctggga cgcggcaggg ttcccggcgg cctggccggg gccgctccgg gcggcgaagg
     6361 agcagggggc gaaggcgccg cagttgccgg ttccgcgcag ccgctgggac tgtgtatcgt
     6421 ctggctgggc aaggtgacga gcaaggaaga ggcggcgcgg ctcgcccagg aggccaaggt
     6481 cgatattctc gccacgtatg agatcgatgt gaagctcgcg cgaaacagca acttcgtcaa
     6541 caacacaacg ctgctccgca tcacgccgac caagaagggc gagccgattt tcgcctccaa
     6601 ggccctcaac aacctgcagg tggtcaatca gaagcaggcg accgagaacc ccgtcgataa
     6661 ggagatcacc cgcgcgatgg aggcgctcga caaggtttgc aaggccgcgc cgctgcccgc
     6721 gctcacggca gaacaggtga aaggccaact ggcgagactc gtggccgaga agccggccga
     6781 tccgctgccg gtcatcgtcg aggcccgcta ttacgtggcc aaggggctgc tgcccgaggc
     6841 cgacatgacg gccgcggcga tcggcctgat gggggaggcc gaattcgccc gcctgatcgt
     6901 cagtgcgcct gaagccaagg gccaaatcgt gggggctgcc ctgagtctgc ccggagtcgt
     6961 caacctgctc cacggtctga attcggcggc cggcgccgcc ggcgcaaccg cgcggcggcc
     7021 gaatccgcag cccggtgcag cgccggggga agcgccgcct ccccgcggcg gcttgcgagg
     7081 attgttgccg ttcggcagcg gctccggccg ataatatcga gcgagcgaac aaatcccagt
     7141 cagcaagtcc gggtcaagga acgacggatg gcggaataca cgaagtacca gcagaaggtc
     7201 atccgcaact actacgacca ccgcgagggg atcgccctgc agcgggtgca ggagctggtg
     7261 accgagctgt atctggcgac gggcaaaaag cgcgacaagc actgggacag cctggccctg
     7321 cacctgggca agctgggcgt caagcccgac gtgatcgagc acctgcgcag cgaggacaag
     7381 cccgagctcg tcgccacgct cgtgaagaag ctgatggata aaaaataacc cggcgagcaa
     7441 tagcacggcg agcaatagca tggcgagcgg gaagcgtaag cttcccgata agcctgggcc
     7501 gtccgcacgc aacctcatcg gccggctgac accggccgct cgccaccagt aatgcgcacc
     7561 gcctggaatt tccactccgc cggccagctc gtcttcggcc gcggcagctg cgcgcagctg
     7621 ggcacgctgc tgtcgcgccg caagctggcg cgggtcttcg tcgtcgccga cccaaggctg
     7681 caggctgcgg gcctcgtcga acaggtcagc cggccgctgc gcgagtccgg catcgcggcg
     7741 caagtgtttg cgggcggcga accggagccg gcggtggcca cggctacaag agcggccgat
     7801 gtggcgcggc agttcggccc cgactgcgtc ctgggtttgg gcggcggcag caacatggac
     7861 ctggccaaga tcgtggccgt gctgctggcc cacggcggcg agcccgcgcg gtatttcggt
     7921 ttcgacaacg tgcccggccc ggtcgcgccg ctggtttgcg tgccgaccac cgccggcacc
     7981 ggcggcgaag tctcgcacgc cgccgtgctc accgacacgg ccaaccacat caaggtcagc
     8041 acgctcagcc agcacctgcg gccgctcttg gccgtggtcg atccggccct gaccgacaac
     8101 tgcccccagc aggtgaccgc cgacagtggc atcgacgccc tgacgcacgc gatcgaggct
     8161 tacacggcga tcgactttga ccagctcgcc gcgccggaca gcgctccggt ggcctacgag
     8221 ggccgatatc cgctcgggga atgcctggcg gagaaggcga tctcgctgat cggccagcac
     8281 ctcgtggcag ccgtgaccga tggcggtaac cgcgccgccc gcgacggcat ggccctggcc
     8341 gcgacgctgg ccggcctggc gttctcgaac tgcgctgtgg ccgtcgttca cgcgctggaa
     8401 tatccgctgg ggggcgcggt gcatgtctcg cacggcgcgg gcaacggact cctgctgccg
     8461 ttcgtgatgc gctacaacct gcccgtgcgg c
//
LOCUS       DASRJB010000191         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1180509, whole genome shotgun sequence.
ACCESSION   DASRJB010000191 DASRJB010000000
VERSION     DASRJB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180509"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFB80_05350"
     CDS             <1..1173
                     /locus_tag="VFB80_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN33220.1"
                     /translation="IFAARAAAELERQQAEESLQASQAALVESEERFRQMADAIRDVF
                     WLYDWPQRRLLYVSPAFEAIWGRSPQWVYDEPFRWLESVHEDDRARAGSMPLPPPGPG
                     KVFDEIYRIRRPDGSVAWLHDRRYAVADSRGQCLRIVGVSEDITQRHEAEERIQQQVA
                     QLAHLSRLSTVGELVASISHEVNQPLYAIANFSSAIASALESRPPPLDSIRQLSGEIG
                     KSVDRAGEIIRRVRSYVVRGTPERRPMDLRAVVHDSAALLAAEARRRRVEIRLELGEP
                     SARLLADPLAIQQVLVNLLRNALDSLAQATGPRLVAISSRAAAGAVEIAVSDSGCGLS
                     PADEQNLFTPFYTSKIDGMGLGLPISKTIVEAHSGRIWAERNPGGGTTVRFTLPVG"
     gene            complement(1189..2520)
                     /locus_tag="VFB80_05355"
     CDS             complement(1189..2520)
                     /locus_tag="VFB80_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN33221.1"
                     /translation="MHLDLDDTIAAIASAPGGAARGIVRLSGPAAIGCTLAVFQPAQP
                     VDPAAVAAPSVFSGAVRLANLGAVPARAYLWPTARSYTRQPAAELHLIGSPPIVEAAL
                     AAICVAGARLARPGEFTLRAFLAGRIDLTQAEAVLGVIDATGQASLHTALAQLAGGLA
                     QPLAKLRESLLDLLAHLEAGLDFVEEDIEFIAAAQLQAQLASAEEQLAQIAAQLAGRG
                     AAGELPRVVLAGPPNAGKSSLLNALAGEGAAIVSPIAGTTRDFVARRLRIGDCECLLI
                     DTAGLDEAIPAGELATRAAAATREQLQQADLVVHCRDALLQPEADVQGPGLMAWTKCD
                     LLAAGAALPEGGIATSSRTGQGLEKLNRAIAERLVSSGAVASTASRCRESVRLAAAAL
                     WRASSAARAEAGQELVAAEVRLALDELGQVVGAVYTEDLLDRVFSRFCIGK"
     gene            complement(2527..>3479)
                     /gene="yidC"
                     /locus_tag="VFB80_05360"
     CDS             complement(2527..>3479)
                     /gene="yidC"
                     /locus_tag="VFB80_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZN33222.1"
                     /translation="PNEKLTHEYRVFFGPKDPNVLRHYSLDRFIEYGWSIFGYPAYWL
                     QQLLNLLHSATHNYGISILLLTVMVRSCMIPISLRQARMAAKMQELAPEVQKIKDRYP
                     DDALKQHAAIQELYKKHNFNMFGGCLPVFLQLPIFIGLYRCLSVNIELRDAALIPAIS
                     WASNLAGPDRLFEWKSWTPGFIGNEADGYFGPFFNVLPLITVVLFLIQQKMITPPATD
                     EQTKMQQQMMTYMTVLMGIMFYKVPAGLCLYFITSSLWGICERKLLPKSKPKGAGSSD
                     SGGGVPAKLPPKPASPNGSAKDKDRDSVKEKLARRARERR"
BASE COUNT          526 a         1184 c         1211 g          558 t
ORIGIN      
        1 atcttcgcgg cgcgggccgc ggccgagctg gaacggcagc aggccgagga gtcgctgcag
       61 gccagccagg cggccctggt cgaaagcgaa gagcggttcc gccagatggc cgacgcgatc
      121 cgcgacgtgt tctggctcta cgactggccg cagcggcggc tgttgtacgt cagcccggcc
      181 ttcgaagcga tctggggacg ctcgccgcag tgggtttacg acgagccttt ccgctggctg
      241 gagtccgtgc acgaagacga ccgggcgcgg gcgggctcga tgccgctgcc gccgcccgga
      301 cccggcaagg tcttcgacga gatctatcgc atccgccggc ccgacggcag cgtggcctgg
      361 ctgcacgacc ggcgctacgc cgtcgccgac tcccgcggcc agtgcctgcg gatcgtcggc
      421 gtgtccgagg acatcaccca gcggcacgag gccgaggaga gaattcagca gcaagtcgcg
      481 cagctcgcgc acctctcgcg gctctcgacg gtcggcgaat tggtcgccag catctcgcac
      541 gaggtgaatc agccgctgta cgccattgct aatttttcgt cggcgatcgc gtcggctttg
      601 gagagccggc cgccgccgct ggattcgatc cggcagctca gcggcgagat cggcaagtcg
      661 gtggaccggg ccggcgagat tatccgccgc gtgcggtcgt atgtcgtccg cggcacgccc
      721 gagcggcggc cgatggacct gcgcgccgtc gtccacgatt cggcggcgct gctggccgcc
      781 gaggcccgcc gccgccgcgt ggagattcgc ctcgagctgg gcgagccgtc cgcgcggctc
      841 ttggccgatc cgctggcgat ccagcaggtg ctcgtgaatc tgctccgcaa cgctctggat
      901 tcgttggcgc aggccaccgg cccgcggctg gtcgccattt cgtcgcgagc cgcggccggc
      961 gccgtcgaga tcgccgtcag cgattcgggc tgcggcctgt cgcccgccga tgaacagaat
     1021 ctctttacgc cgttctacac gtcgaagatc gacggaatgg gcctgggcct gccgatcagc
     1081 aagacgatcg tcgaagccca cagcggccgc atctgggccg agcggaaccc cggcggcgga
     1141 acgacggtgc ggttcacgct gccggtgggg tagggcagtg ttcccgattt acttcccaat
     1201 acaaaaccgg ctgaacactc ggtccagcag gtcctcggta tagaccgcgc cgacgacctg
     1261 gccgagctca tctagagcta gccggacctc ggcggcgacc agttcctggc ccgcttccgc
     1321 gcgggcggcc gagctggcgc gccagagcgc ggcggcagcc aggcggacgc tctcgcggca
     1381 gcgggaggcc gtgctggcga ctgcaccgct cgataccagg cgctccgcga tcgcgcggtt
     1441 cagcttctcg agcccctggc ccgtgcggct gctggtcgcg atgccgcctt ccggcagcgc
     1501 cgcgccggcg gcgagcaggt cgcacttggt ccaggccatc agtccaggcc cctgcacatc
     1561 cgcttcaggc tggagcagag cgtcgcggca atgcacaacg aggtcggcct gctgcaactg
     1621 ctcgcgcgtg gcggcggccg cgcgggtggc cagttcgccg gctgggatgg cttcgtccag
     1681 accggccgtg tcgatgagca ggcactcgca gtcgccgatc cgcagccgcc gggccacaaa
     1741 atcgcgggtc gtgccggcga tcggcgagac gatcgccgcc ccttcgccgg ccagcgcgtt
     1801 gagcaggctg cttttgccgg cgttgggcgg cccggccagc accacccgcg gcaattcgcc
     1861 cgccgcgcct cgcccggcca gctgcgccgc gatttgcgcg agctgctctt ccgccgaggc
     1921 cagttgcgct tgaagctgcg cggcggcgat gaattcgatg tcttcctcca cgaagtcgag
     1981 tccggcctcc aggtgcgcca gcaagtccag cagcgactcc cgcagtttgg ccagcggctg
     2041 cgccagcccg ccggccagct gggccaaagc ggtatgcagc gacgcctggc cggtggcgtc
     2101 gatcacgccg agcactgcct cggcctgcgt cagatcgatc cggcccgcca ggaaagcccg
     2161 cagcgtgaac tcgcctggcc gcgcgaggcg tgcgccggcg acacaaatcg cggccagcgc
     2221 cgcctccaca atcggcggcg agccgatcag gtgcagttcc gccgccggct gccgcgtgta
     2281 gctccgcgcg gttggccaga gatacgcccg cgccggcaca gcgccgaggt tcgccagccg
     2341 cacagcgccg gaaaagacgc tgggagccgc caccgccgcg ggatcgaccg gctgtgccgg
     2401 ctgaaacacc gccagcgtgc agccgatcgc cgccgggccg ctcagccgca cgatgccccg
     2461 tgctgcgccg ccgggggccg aagcgatcgc ggcaatggtg tcgtcgaggt ccaggtgcat
     2521 gcgcgcctag cgccgctcgc gggcccgccg ggcgagcttt tccttcacgg agtcccggtc
     2581 cttgtccttg gccgagccgt tggggctggc gggcttgggc ggcagcttcg cgggaacgcc
     2641 gccgccgctg tcgctgctgc cggctccctt cggcttgctc ttgggcagca gcttccgctc
     2701 gcagatgccc cacaggctgg aggtgatgaa atacaggcac agtcccgcgg gcaccttgta
     2761 gaacatgatg cccatgagga ccgtcatgta ggtcatcatc tgctgctgca tcttggtctg
     2821 ctcgtcggtt gccggcggcg tgatcatctt ttgctggatc aggaacagca cgaccgtaat
     2881 cagcggcagc acgttgaaga acgggccgaa atagccgtcg gcttcgttgc cgatgaagcc
     2941 gggggtccag ctcttccact cgaacagccg gtcggggccg gccaggttgc tggcccagga
     3001 aatcgccgga atcagggccg cgtcgcggag ctcgatgttg acgctcaggc agcggtacaa
     3061 gccgatgaag atcggcagct gcaggaacac gggcaggcag ccgccgaaca tgttgaagtt
     3121 gtgcttcttg tagagctcct gaatggcggc gtgctgcttg agggcgtcgt cgggatagcg
     3181 gtccttgatc ttctgcactt cgggggcgag ctcctgcatc ttggccgcca tgcgggcctg
     3241 ccgcagcgag atcgggatca tgcaggagcg gaccatcacc gtcagcagga ggatcgaaat
     3301 cccgtagttg tgcgtcgcgc tgtgcagcaa gttcagcaac tgctgcaacc aataggcggg
     3361 atagccgaaa atgctccagc cgtactcgat gaaccggtcc agcgagtaat gacgcaggac
     3421 gttggggtct ttcgggccga agaagacgcg gtactcgtgc gtgagcttct cgtttggct
//
LOCUS       DASRJB010000192         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4004166, whole genome shotgun sequence.
ACCESSION   DASRJB010000192 DASRJB010000000
VERSION     DASRJB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4004166"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFB80_05365"
     CDS             complement(<1..615)
                     /locus_tag="VFB80_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33223.1"
                     /translation="MPIDVTCNKCKTRFQVSEKFAGKKGPCPKCKNIITVPDKQQEVV
                     IHAPEVAGPVDSTGAAVIKPLARKEVRLTTPVIVGIVGAVVIVLFAALILRVSTKPTG
                     PGGFRHYSPLVTILGALVLAPPLAFAGYTFLRDDELEPHRGTDLILRLIAPSVIYPGL
                     WGLYWLAFAYLGSTVQPNWQVLAFVLPAVIALGAFTAQVSLDLEF"
     gene            726..2033
                     /locus_tag="VFB80_05370"
     CDS             726..2033
                     /locus_tag="VFB80_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN33224.1"
                     /translation="MKWLAGAILILVIALLFGFDLLAYAMYVLLAVMLISRYLTKTWS
                     ESLSASRECSLLSAEVGQNVAVVVTLKNTGRWPVAWVLLEDLLPADALIFNPPRLQVG
                     GKRVQLTMLKAAGRKSMLYQLTPTRRGYYQLGPLVLETGDLFGLHRRYRVLTDPQFLM
                     VLPKVLPLAGYDVSSKRPIGEVRMTYRLYEDPTRISGVRAYERGDPMNRVHWRATART
                     GQLHSKVYEPSTVAGATLLLEFHMRAHERKHEPHRSELAVTAAASIANAVYLMNQQIG
                     LVTNGRDAVDRIRQEGWAHDYRSRAAALASASVKDKSDRLAPLIVQTRRGPEQLMRIL
                     ETLARVELTDGLPLPALIDEASSRMPRDATVIAILPPGSRESAAALGNLRRRGFAVTA
                     LVNMFDDYDFAEAAAPLLAEGIGVKQLKDEGSIMEICRRLALR"
     gene            2114..>4006
                     /locus_tag="VFB80_05375"
     CDS             2114..>4006
                     /locus_tag="VFB80_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZN33225.1"
                     /translation="MSRRPPETLADYLAIAVCPFLIFLLVASLMFFLVEVFYEGEFKL
                     RLQFIMAMFCMAIVCIARIAMEEGSSYATLYALPLGGAVAFAVSRLAPGGLLISWPLM
                     GIVWWAAHKLTWDCTLVDDSQEASGQGLLQEMGLDPLAAGVPRGPAGTTVRPQLLEAT
                     TGNSEPEKSWWEKLLDPDRRPHSPGVWVVYFSLAALPLFGIGGWFVPTGDAEARTRVF
                     QFMVVYVGSGLGLLLATSFLGLRRYLRQRNLEMPAQMTATWIAVGGGLIAVTLLLATI
                     LPRTKAEHSLTALPFAITSAARRASQYAFGPEGTSDSDPDAATTDAKDNQSSDRSGGT
                     KGESKAGQGDKSKSKSKGEGDGKSDGEQGGGQSNGGKAQSGKAGEKSKGKGKAGEKSE
                     GKTGEQQEKSKEGGESSTGGQKKKKDDDQQQNQSNNSAEQSSQENSQSQSPAEQPQSS
                     SPPLLSNLNIGQTLLTLIKWLFYLALLIAAVVAGWIYREQLAAAWQKLLAELRDLWSR
                     WFGKPEAAEQAAAAAALPPPPRPFAAFADPFAGGEQTRMPWPELIRHTFAAAEAWARE
                     HGCPRHPDQTPHEFAQALGALEPQIAAPIQSLAAWYAQLAYAPRAASAGNLQPLRQLW
                     TEMRAST"
BASE COUNT          690 a         1351 c         1338 g          627 t
ORIGIN      
        1 gaactccagg tcgaggctga cttgggcggt gaaggccccg agggcgatca ccgcgggcaa
       61 gacgaacgcc agcacttgcc agttcggctg cacggtgctc cccaggtagg caaacgccag
      121 ccaatacagc ccccacaggc ccgggtagat aacgctgggg gcgatcagcc gcaggatcag
      181 gtcggtgccg cggtgcggtt ccagctcgtc gtcgcggagg aacgtgtagc cggcgaaggc
      241 cagcggcgga gcgagtacca gcgcgcccag gatcgtaacc aagggagagt agtggcgaaa
      301 tcctccgggg ccagtcggct tcgtggagac tcgcaggatc agggccgcga agagcacgat
      361 cacgaccgcc cctacgatgc cgacgatgac cggcgtggtc aggcggacct ccttccgggc
      421 gagcggcttg atgaccgccg cgccggtgct gtcaaccggg ccggcgacct ccggggcatg
      481 gatcaccacc tcctgctgct tgtccgggac ggtgatgatg ttcttgcact tggggcaggg
      541 ccccttcttg ccggcgaatt tctcgctgac ctgaaagcgg gtcttgcact tgttgcaggt
      601 gacgtcgatg ggcatgcggg cagaccggca gcggttcgat tgggggatcg ctggctagtt
      661 tcgcacagcg gcccggcggt gggaagtgct tcgattccga accgggctca agttaggata
      721 gaggcatgaa gtggctggcc ggcgcgattc tgatcctcgt catcgcgctc ctcttcggct
      781 tcgatctgct ggcgtacgca atgtacgtcc tcctggcggt gatgctcatc agccgctatc
      841 tgaccaaaac ctggtccgag tcgctgtcgg ccagccgcga gtgcagcctg ctctcggcgg
      901 aggtcgggca gaacgtagcc gtggtcgtca cgctgaaaaa cacgggccgc tggccggtgg
      961 cctgggtgct gctcgaggac ctgctgcccg cggacgcgct gatcttcaat ccgccgcggc
     1021 tgcaagtcgg cggcaagcgg gtgcagctga cgatgctcaa ggccgccggc cgcaagtcga
     1081 tgctctatca gctcacgccg acgcgccgcg gctattacca gcttgggccg ctggtgctgg
     1141 agaccggcga cctgtttggc ctgcaccggc ggtaccgcgt gctcaccgac ccgcagttcc
     1201 tgatggtcct gcccaaggtg ctgccgctgg ccggctacga cgtcagctcc aagcggccca
     1261 tcggcgaagt gcggatgacc tatcggctgt acgaagaccc gacgcggatt tccggcgtgc
     1321 gggcctacga gcggggcgat ccaatgaacc gcgtgcattg gcgggcgacc gcgcggaccg
     1381 gccagctgca cagcaaggtc tatgagccct cgaccgtcgc cggggcgacg ctgctgctgg
     1441 aatttcacat gcgggcccac gagcggaagc acgagccgca ccgcagcgag ctggccgtga
     1501 cggccgcggc gtcgatcgcc aacgcggtgt acctcatgaa ccagcagatc ggcctggtga
     1561 ccaacggccg cgacgcggtc gatcgcatcc gccaggaggg ctgggcccac gactaccgct
     1621 cgcgggccgc ggccctcgcc tcggccagcg tcaaggacaa gagcgaccgg ctcgcgccgc
     1681 tgattgttca gacccgccgc ggtccggagc agctgatgcg aatcctggaa acgctggccc
     1741 gcgtggagct gaccgacggc ctgccgctgc cggccttgat cgacgaggct tccagccgga
     1801 tgccgcggga cgcgaccgtg atcgccatcc tgccgccagg ctcgcgggaa tcggcggccg
     1861 cgctgggcaa tctccgccgc cgcggcttcg cggtaaccgc gctggtcaac atgttcgacg
     1921 actacgactt cgccgaggcc gccgcgccgc tcctggccga gggcatcggc gtcaagcagc
     1981 tcaaagacga aggctcgatc atggaaatct gccgccggct ggcgttacga tgataacgta
     2041 gagtaagcgt ctcgcttgct cagcgcactt gcagagctga cctgcacaag cgaggacgct
     2101 tgtgctaagc accatgtccc gccgaccgcc tgaaacgctg gccgattacc tggcgatcgc
     2161 ggtctgcccg ttcctcatct tcctgctcgt cgccagcctg atgttcttcc tggtcgaggt
     2221 cttctacgag ggcgaattca aactgcggct ccagttcatc atggccatgt tctgcatggc
     2281 catcgtctgc atcgcccgca ttgcgatgga ggaaggcagc tcgtacgcca cgctgtacgc
     2341 cctgccgctg ggcggcgccg tcgccttcgc ggtctcgcgg ctggcgcccg gcggcttgct
     2401 aatcagttgg ccgctcatgg gcatcgtctg gtgggcggcg cacaagctga cgtgggactg
     2461 cacgctggtc gatgattcgc aggaagcctc ggggcagggg ctgctccagg agatgggcct
     2521 cgatcccctg gccgccggcg tgccgcgcgg gccggccggc accaccgtcc ggccgcagct
     2581 cttggaggcg acgaccggca acagcgagcc ggaaaagtcc tggtgggaaa aactgctcga
     2641 tcccgaccgc cggccgcatt cgcccggagt ctgggtcgtc tatttctcgc tcgccgcctt
     2701 gccgctcttc ggcatcggcg gctggttcgt gccaaccggc gacgccgagg cccgcacccg
     2761 cgtgttccag ttcatggtcg tgtatgtcgg cagcggtctg gggctgcttc tggccacgag
     2821 ctttctcggc ctgcggcgat acctgcggca acggaacctc gaaatgcccg cccagatgac
     2881 ggcaacctgg atcgccgtcg gcggcggcct catcgcggtg acgctgctcc tggcgacgat
     2941 cctgccgcgg accaaggcgg agcattcgct gactgcgttg cccttcgcga tcacctcggc
     3001 cgcccggcgg gccagccaat atgccttcgg ccccgaaggc accagcgact ccgatccgga
     3061 cgcggcgacg actgacgcca aggacaacca atcgtccgat cgctccggcg gtacgaaggg
     3121 cgagagcaaa gcgggccagg gcgacaaatc gaaatccaag tcgaaaggcg agggagacgg
     3181 aaaatcggac ggcgagcaag gcggcggcca atcgaacggc ggcaaggctc agtcgggcaa
     3241 agcgggcgag aagtccaagg gcaaaggcaa ggcgggtgaa aaatccgagg gaaagacggg
     3301 cgagcaacag gaaaaatcaa aggagggcgg cgaatcctcc acgggaggtc aaaagaagaa
     3361 aaaggatgac gaccagcagc aaaaccaatc gaacaactcg gccgagcaga gcagccagga
     3421 gaattcgcag tcccagtcgc cggccgagca gccgcagtcg tcgtcacctc cgctgctctc
     3481 gaacctcaac atcggccaga cgctgctgac gctgatcaag tggctgttct acctggcgct
     3541 gctcatcgca gcggtggtcg ccggctggat ctatcgcgag cagctggccg cggcctggca
     3601 gaagctgctg gccgagctgc gcgatctgtg gtcccgctgg ttcggcaagc cggaagcagc
     3661 cgagcaggcg gcggccgcgg ccgccttgcc tccgccgccg cggcccttcg cggcgtttgc
     3721 cgatcccttt gccggcggcg agcagacccg catgccctgg ccggagctga tccgccacac
     3781 cttcgcggcc gccgaagcct gggcccgcga gcacggctgc ccgcgtcatc ccgaccagac
     3841 gccccatgaa ttcgcccagg ccctcggagc gctcgaaccc cagatcgccg ctcccatcca
     3901 atcgctcgcc gcctggtacg cccaactcgc ctacgccccc cgcgccgcct ccgccggcaa
     3961 cctccagccg ctgcggcagc tctggaccga aatgcgagcc tccaca
//
LOCUS       DASRJB010000193        12713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1181166, whole genome shotgun sequence.
ACCESSION   DASRJB010000193 DASRJB010000000
VERSION     DASRJB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181166"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1048
                     /gene="pyrF"
                     /locus_tag="VFB80_05380"
     CDS             122..1048
                     /gene="pyrF"
                     /locus_tag="VFB80_05380"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZN33226.1"
                     /translation="MTNQATTHFADRLAAAVRQKGNPVLVGLDPRAESLPPGLISGSG
                     PEATARGYIAFCRGVIDVVAPLVAAVKPQAAFFEQLGPAGMTALKTVIDYAASKSLLV
                     ILDGKRNDIGSTAEAYAEAYLGPGDASPWGCDALTVSPYLGDDSLTPFVEIAKARGAG
                     IFVLVKTSNPGGKRFQDIVADGRPLYRHVADYVESLAAQTAGACGYGSVGAVVGATYP
                     EQLAELRAAMPHTWLLIPGYGAQGGTAKDCAAAFDAQGLGAIVNNSRGIIFAHSRKEF
                     KDRFGPSRWQEAVEAATREMIEQLASVRAFIA"
     gene            complement(1038..1535)
                     /locus_tag="VFB80_05385"
     CDS             complement(1038..1535)
                     /locus_tag="VFB80_05385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN33227.1"
                     /translation="MNPPAIRLATESDLSAINDIYNYYVLRSTCTYQLEPETLEARTA
                     WFRNHPPGKYPVTVAEVDGHVAGWGSLSKFRDRAAYDPTVEASVYIRHDLHRRGLGRA
                     LLSDLIERARAAGFHSLLGGASGDQAASLALQESLGFTRVACFKEVGYKFGSRLDVVF
                     MELML"
     gene            complement(1570..3054)
                     /locus_tag="VFB80_05390"
     CDS             complement(1570..3054)
                     /locus_tag="VFB80_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZN33228.1"
                     /translation="MGKPTGFKEFPRRAVPYREPLKRIEDFAEIFTQPPEEHLRLQGA
                     RCMDCGVPFCQSASGCPIDNLIPEWNDLVYQGRWREALDRLHKTNNFPEFTGRTCPAP
                     CEGACVLGITDPPVTIKNIENAIIDRGFAEGWVVPQPPPVRTGKKVAVIGSGPAGLAA
                     AAQLNKVGHSVTVYERADRIGGLLMYGIPNMKLDKGVVQRRVNLLAEEGIEFVTNADV
                     GNRESPHFVDPKELLANSDALLLSTGATRPNDLPIPGRGLAGIHFAMQFLTANTKSLL
                     DSGHLNGQFISAKDVDVIVIGGGDTGTDCIGTSMRHGCRSLVNFELLPQPPKDRAPDN
                     PWPQWPRILRTDYGHQEVAAKFGADPRTFCVMSKEFVGDDEGRVAGIRTVQVEWNKVD
                     GQWKLKELQGTDKFWPAQLVLLAMGFKGPEQYVSELLGLEHDPRSNYKAQHGRFATSL
                     PKVFAAGDCRRGQSLVVWAINEGRGAARAIDEFLMGSSDLPAPA"
     gene            complement(3047..7630)
                     /gene="gltB"
                     /locus_tag="VFB80_05395"
     CDS             complement(3047..7630)
                     /gene="gltB"
                     /locus_tag="VFB80_05395"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZN33229.1"
                     /translation="MMLELENGLPKKHGLYDPAFEKDSCGVGFIAHIKGVRSHSIVRD
                     ACAALRAMDHRGACGCEANTGDGAGILTAIPDELMRAEARRLFGSELPPIGSYAVAQV
                     FLPRDDAERKTCKSTLEKYVAAQGQKLVGWRPVPTDVKGADIGATAAAAEPVIEQLIV
                     AAGDSIERTAFCRQLYLIRKQAFHALNKQALKQRNMYYVASFSSRIIIYKGQLTSHQV
                     VPYFPDLADPRYTSHLAMIHSRFSTNTFPSWERAQPMRFMSHNGEINTLRGNINWMSA
                     REGLLESELFGKDLRKLLPVTDLSTSDSGVFDNVLELLLLGGRSLPEAVMMMIPEAWQ
                     NHESMPEYKRAFYEYHSCLMEPWDGPASVVFCDGRYIGAVLDRNGLRPSRYYLTTDDR
                     VIMASEVGVLPVDPKIVKAKGRLQPGRMFLVDFERGALIPDEEVKKEVATKRPYAQWL
                     QEQRVELRDLPLVDQSHGFEPETLLARMQAFGYSTETMQFMLLPLVGEKRDPVGSMGN
                     DSALACLSDQPRMLYDYFKQLFAQVTNPPIDSIREEVIMSLECYVGPERNLLATTPEQ
                     AHRLLLPHPILTNEELAAINHIEHRGWKSKLIDITFARTEGEAGLVKRLDRICREAEK
                     AIDDGYQLIVLSDRGVSAERVPVSSLLAVGAAHHHLIRQAKRTRIGIVLESGEAREVH
                     HHCLLVGYGADAINPYVAFESLWQAQRDGLLPAKYSDNDIVHAYQKAVAKGMLKVMSK
                     MGISTLQSYKGAQIFEAVGLNDDVIQKCFAGTASRIKGCDFSILAKEALRRHEIGFPQ
                     RAEQRLAVLPNFGEFHWRAEGERHMWDPQAIADLQVAARENSADAYAKFSKHVNEELS
                     RRCALRGLLKLKTGVNRPVPLAEVEPAKEIVKRFCTGAMSFGSISAEAHETLAIAMNR
                     IGGKSNTGEGGEDPERFKPLPNGDSKRSAIKQVASGRFGVTINYLTNADELQIKMAQG
                     AKPGEGGELPGHKVDETIARIRYSTPGVGLISPPPHHDIYSIEDLKQLIHDLKNSNPD
                     ARVSVKLVSEVGVGTVAAGVAKAHADHILISGDTGGTGASPITSIKHAGLPWELGIAE
                     THQTLVMNDLRSRVVLQTDGQIKTGREVVMAALLGAEEIGFSTGPLITLGCIMMRKCH
                     LNTCPVGIATQDPVLRQKFTGQPEHVINYLFLIAEEVRQHMAELGFRTFSEMIGRVDC
                     LETNDAIRHWKAAGIDLTPILTPAQKPHPDVEVYHTRGQDHGLEGALDKQLITECQKA
                     IKRRESVVLRRTIRNTNRTVGTMLSHEIAKRWGDDLLPDDTIRIKFSGSAGQSFGAFL
                     AKGVTLELEGDANDYVGKGLSGGRLALYPPAGSLFKPEDNIIIGNVALYGATSGQAFF
                     RGRAAERFAVRNSGAHAVVEGVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGIAYVWD
                     PLDEFTARCNLGIVELERIETPQDLGELLHLVELHRQYTDSTLAEKVLAQWPEVTAQF
                     WKVMPVDYKRVLAERAKHDEEVEAGVHGVPSHG"
     gene            complement(7697..8677)
                     /locus_tag="VFB80_05400"
     CDS             complement(7697..8677)
                     /locus_tag="VFB80_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN33230.1"
                     /translation="MHLKSLKVFCDVVGRRSFSRAASENGISQSGASQIVNGLEEHLG
                     VKLIDRTKRPFVLTPEGEAYYEGCRKLVQRYFALEEEVRTLHEEVAGRVSVASIYSIG
                     LSHMNRFVQRFLQAYPKANVRVQYQHPRRVYELVETNQVDLGLVSYPRSNRTIKATVW
                     RQEPMALVCSPGHKLALRDRVTLEDLHGLEIVAFDTDLEIRNEIDRALGGRGIEVRVM
                     MEFDNTETIKRAVEIDAGVSLLPLPTVEREVAAGALVARPLAGCELKRPIGIIQRRGT
                     ELGKTASRFMHLLLKEPVHSRGIAGPESTALEPGKTEASKPAESLAPVAS"
     gene            complement(8876..12694)
                     /locus_tag="VFB80_05405"
     CDS             complement(8876..12694)
                     /locus_tag="VFB80_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HZN33231.1"
                     /translation="MALSAVAQHDYEQVEQLERSRFDAKHAALERTYKAARESVFCQY
                     EAAGFKLAKEEERALAAADQEYEGAREAGKTLCQHQSQKILAAYKLDSQRPRSEFAQF
                     KQQCEVRQAEIQSLITQAQKIVRRRCPWPDDANPPPPPAAAAPQRASAQHVERFTAAM
                     QRAYALVYALQHQRAARFLEDGWPVLIFLLALPLVAYPAWLLLAAFGWGWVIAAALIV
                     PLCLALAARQIARPIARRQTLQQLPDFQVAVALAREELAAAQAAAHLEGERKYDHLGE
                     QRDLDLAAAKAEWRQARRALKAEHAARTEQLTQAFTVRREALEETYERQFQQLEERHP
                     PQLARVEQDYAEDAARRGEQHRALQTANDARFAGEWNSLAKRWSAGLADFQSAARAMN
                     ADCDAHFPAWDAVEPQRQAAVAAAATALCIGRHELRLDQLPGGLPAAEQLEQAPAAVR
                     LPVVLSYPQRPSLVLEAEGASRDAAALVLQNTMLRLLTTFPPGKVRFTIIDPVGLGQN
                     FSAFMHLADFDPRLVTSRIWTESAHINQRLADLTEHMENVIQKYLRNEFASIQDYNAD
                     AGEVAEPYQILVVANFPANFSDEAARRLAAIAASGARCGVFTLISTDQKLALPRNFDL
                     ADLERHSVTLVWDAAEQRFAWRDADLRGLRLVLDSPPGDERFTELVQAVGQRAKETAR
                     VEVPFEAVLPAADQWWKADSRRGIEVPLGRAGAKKLQYLRLGRGTSQHVLIAGKTGSG
                     KSTLLNALITNLAAHYSPNELHFYLIDFKKGVEFKAYATHQLPHARVIAIESEREFGM
                     SVLERLDVELKRRGDLLRQAGVQDVAGYRNANREAVLPRILLVIDEFQEFFTSDDKIS
                     HDASLLLDRLVRQGRAFGIHVLLGSQTLAGAYSLARSTLGQMAVRIALQCSESDAHLI
                     LSEDNTAARLLNRPGEAIYNDANGLVEGNHPFQVVWLSDHERESQLRRAAELAQLRGL
                     KCDPPTIFEGNMAADPAANAALRDVLQAAQPTAAGAPRAWLGAAVAIKDPTSAVFRRQ
                     TGSHLLVVGQQEELALGMLANGALALAAHGGILWVLDGAPPDSPAAGFWNKLCGPLAS
                     AARVIAPREAGAAVQQLAEEVERRMSRGDAPGEAIFLFIHHLARFRELKKSDDFGLSF
                     DDRPAASADKHLTAILREGPAVGVHALIWCDSYSNANRWLDRQSLRDIDQRVLLQMSA
                     SDSSQLMESPAASKLGTHLAYYYSEELGRAEKFRPYGVPASEWLTWAQQRLAQRTATL
                     STQTDPSLLN"
BASE COUNT         2100 a         4224 c         4068 g         2321 t
ORIGIN      
        1 gcgaaagcgc gctggtcgtc gaggccgacg cccgctggga tttcgggcag ctcctgcagc
       61 cagaggcgtg ggacgtgcga gaatacccgc cggccttcgt ggggatttac atcaagtaaa
      121 tatgacgaac caagcgacaa cccactttgc cgaccggctc gccgctgccg tccgtcagaa
      181 aggcaacccg gtgctcgtcg gcctcgaccc gcgggcggaa agtttgcccc cgggattgat
      241 ctccggcagc ggcccagagg cgacagcccg cggctacatc gcgttttgcc ggggtgtgat
      301 cgacgtcgtc gcgccgctgg tggctgcagt aaagccgcag gcggcgttct tcgagcagct
      361 tggtccggcg ggcatgacgg cgctgaagac agtcatcgac tacgcggcgt ccaaaagcct
      421 gcttgtcatc ctcgacggca agcgcaacga catcggcagt acagccgaag cttatgctga
      481 ggcgtatctg gggccgggcg atgcgagccc ctggggctgc gacgcattga ccgtcagccc
      541 ctacctgggc gacgacagcc tgacgccgtt tgtcgaaatc gcgaaggccc gcggcgcagg
      601 gatcttcgtc ctcgtgaaga ccagcaatcc cggcggcaag cggttccagg acattgtcgc
      661 ggatggccgc ccgctctatc gccacgtcgc cgactatgtc gagtcgctcg cggcacagac
      721 ggccggagca tgcggctacg gcagcgtcgg cgcggtcgtc ggcgcgacct atccggagca
      781 gctggccgaa ttgcgggccg cgatgccgca cacctggctg ctgatccccg gctacggcgc
      841 gcaaggcggc acggccaaag actgcgccgc ggccttcgac gcccagggct tgggcgcgat
      901 tgtcaacaac agccgcggga tcatattcgc ccacagccgc aaggaattta aagaccgctt
      961 cggtccgtcc cggtggcaag aagcagtcga agcggcgacg cgggagatga tcgagcaatt
     1021 ggcgagcgtg cgggcgttca tagcatgagc tccatgaaca ctacgtccaa tcgactgcca
     1081 aacttgtagc cgacttcctt gaaacaggcc acgcgcgtga agccgaggct ttcctgcagg
     1141 gcgaggctcg ccgcctggtc gccgctcgcg ccgcccagca gcgagtgaaa gccggcggcg
     1201 cgggcccgct cgatgagatc ggacaagagc gcgcggccca gcccgcggcg gtgcaggtcg
     1261 tgccggatat agacggaggc ctcgaccgtc ggatcgtagg ccgcccggtc gcggaacttg
     1321 ctgagcgatc cccaaccggc gacgtggccg tccacctcgg caaccgtcac agggtatttg
     1381 ccgggcgggt gattgcggaa ccaagccgtg cgggcctcaa gggtttccgg ctccagttga
     1441 tacgtgcagg tcgagcggag gacgtagtag ttgtagatgt cgttgatcgc ggacaggtcg
     1501 gattcggtgg ccaggcggat tgcaggaggg ttcatggtga aacgcagaca agaaggtccg
     1561 ccccacggtt cacgccgggg caggcaaatc gctcgaaccc atcaggaact cgtcgatggc
     1621 gcgggccgcg ccgcgccctt cgttgatggc ccacacgacg agcgattggc cgcggcggca
     1681 gtcgccggcg gcaaacacct tgggcaggct ggtggcgaac cgcccgtgct gggccttgta
     1741 gttcgagcgc gggtcatgct ccaaaccaag cagctccgag acatattgct ccgggccttt
     1801 gaagcccatc gccagcaaga cgagctgggc cggccagaac ttgtcggtgc cctgcagctc
     1861 cttgagcttc cactggccgt caaccttgtt ccactcgacc tgcaccgtgc gaatgccggc
     1921 gacgcggcct tcgtcgtcgc ccacgaactc cttgctcatc acgcagaacg tccgcggatc
     1981 ggccccaaac ttcgcggcga cctcctggtg accataatct gtccgcagga ttcgcggcca
     2041 ctgcggccac gggttgtcgg gggcccggtc cttcggcggc tgcggcagca gttcgaaatt
     2101 cacgaggctc ctgcacccgt ggcgcatcga cgtgccgatg cagtcggtgc ccgtgtcgcc
     2161 gccgccgatg acgatcacgt ccacgtcctt ggcgctgatg aattgcccgt tcagatggcc
     2221 gctgtcgagc aggctcttgg tgtttgcggt gaggaactgc atggcgaagt gaatgccggc
     2281 gaggccgcgg ccgggaatcg gcaggtcgtt gggccgcgtc gctcccgtgg agagcagcag
     2341 cgcgtcgctg ttggccagca attccttggg atcgacaaag tgcggcgact cgcggttccc
     2401 cacatcggca ttcgtgacga actcaatgcc ttcctccgcc agcaggttca cccgccgctg
     2461 caccactcct ttgtcgagct tcatattggg gatgccgtac atgagcaggc cgccgatgcg
     2521 gtccgcccgc tcatagaccg tcacgctgtg gccgaccttg ttcagttgcg ccgccgctgc
     2581 caggcccgcc ggcccggagc cgatgaccgc gaccttcttg ccggtgcgca ctggcggcgg
     2641 ctgcggcacg acccagccct cggcaaagcc gcggtcgata atcgcgttct cgatgttctt
     2701 gatcgtgacg ggcgggtcgg tgatgcccag cacgcaggcc ccttcgcagg gtgcggggca
     2761 cgtgcggccg gtgaactccg ggaagttgtt cgtcttgtgc agccggtcca gtgcctcgcg
     2821 ccagcggccc tgatagacca ggtcgttcca ttccggtatc agattgtcga tcgggcaacc
     2881 cgacgcactc tggcagaacg gcacgccgca gtccatgcac cgcgcccctt gcaggcgcag
     2941 atgctcctcc ggtggctgcg tgaagatctc ggcaaaatcc tcgatccgct tgagcggctc
     3001 gcgatagggc accgcccttc gcgggaactc cttgaagccg gtgggcttac ccatgactcg
     3061 gcactccatg cacgccggct tcgacttctt cgtcgtgctt ggcgcgttcg gcgaggacgc
     3121 gtttgtaatc gacgggcatc accttccaga actgcgccgt tacttcgggc cactgggcca
     3181 gcaccttctc ggccagcgtc gaatccgtgt attggcggtg caattcgacc aggtgcagca
     3241 gctcgcccag gtcttgcggc gtctcgatcc gctcgagctc gacgatgccc aggttgcagc
     3301 gggcggtgaa ttcgtccagc ggatcccaga cgtaggcaat gccgccgctc atgcccgcgg
     3361 cgaaattccg cccggttggg ccaagcacga cgacgcggcc gccggtcatg tattcgcagc
     3421 cgtgatcgcc gacgccctcg acgaccgcgt gtgcgccact gttgcggacg gcgaaccgct
     3481 cggccgcgcg cccgcggaag aaggcctggc cgctcgtcgc gccgtacagg gcgacgtttc
     3541 cgatgatgat gttgtcctcg ggcttgaaca gcgaaccggc cggcggatag agggccagcc
     3601 gcccgccgga cagacccttg ccgacgtagt cgttggcgtc accttccaat tccagcgtga
     3661 cgcctttggc cagaaacgca ccaaaactct gacccgcgga gccgctgaac ttgatgcgga
     3721 tcgtgtcgtc ggggagcaaa tcgtcccccc accgcttggc gatctcgtgg ctgagcattg
     3781 tgccgacggt gcggttggtg ttgcggatcg ttctgcggag cacgacactt tcgcggcgct
     3841 tgattgcctt ctggcactcg gtgatcagct gcttgtcgag ggccccctcc aggccgtggt
     3901 cctggccccg ggtgtgatag acctcgacgt ctggatgggg cttttgggca ggagtcaaaa
     3961 tcggcgtcaa gtcgatgccg gctgctttcc agtgccggat ggcgtcgttc gtctccaggc
     4021 aatcgacgcg gccgatcatt tcactgaacg tgcggaatcc cagctccgcc atgtgctgcc
     4081 gcacttcttc cgcaatcagg aacaggtagt tgatgacgtg ctccggctgc ccggtgaatt
     4141 tctgccgcag caccgggtct tgggtggcga tgcccacggg acacgtattg aggtggcact
     4201 tccgcatcat gatgcagccc agcgtaatca gcgggccggt cgagaagccg atttcctcgg
     4261 ctcccaggag agcggccatc accacctcgc ggcccgtctt gatttggccg tcggtctgga
     4321 gcacgacgcg gctgcgaagg tcgttcatca ccagcgtctg gtgcgtctca gcgatgccca
     4381 actcccaggg cagtccggcg tgcttgatgc tggtgatcgg cgaagcgccg gtgccgccgg
     4441 tatcgccaga aatcagaatg tgatcggcgt gggccttggc cactccggcg gcgacggtgc
     4501 caacgccaac ctcgctaacc agcttgacgc tgacgcgggc gtcagggttg ctgttcttca
     4561 ggtcgtggat gagctgcttg aggtcctcga tcgagtagat gtcgtggtgc ggcggcgggc
     4621 tgatcaaacc gacgcccggc gtgctgtagc ggatgcgggc gatcgtctca tcgaccttgt
     4681 gcccgggcaa ttcgccccct tcgcccggct tggccccctg ggccatcttg atctgcagct
     4741 cgtcggcgtt ggtcaggtag ttgatcgtga cgccgaagcg accgctggcc acctgcttga
     4801 tcgccgagcg cttgctgtcg ccgttgggca gcggcttgaa ccgttcgggg tcttcgcctc
     4861 cttcgccggt gttcgatttg ccgccaatgc ggttcatggc gatcgccagc gtctcgtggg
     4921 cctcggccga aatgctgcca aagctcatcg cgccggtgca gaaccgcttg acgatttcct
     4981 tggccggttc gacttcggcc agcggcacgg gccgattgac gcccgtcttc agcttcagca
     5041 agccccgcag cgcgcaccgc cgcgacagct cttcgttgac gtgcttggaa aacttggcat
     5101 aggcatcggc gctgttctcg cgggccgcta cttgcaaatc cgcgatcgcc tgcgggtccc
     5161 acatgtgccg ctcgccctcg gcccgccagt ggaactcgcc aaaattcggc agcaccgcca
     5221 gccgctgctc agcccgctgc ggaaagccga tctcgtgccg ccgcagggcc tccttcgcca
     5281 ggatcgagaa atcacagccc ttgatccggc tggctgtgcc cgcgaagcac ttttgaatca
     5341 cgtcgtcatt caggccgacc gcctcgaaga tctgcgcccc tttgtacgac tggagcgtcg
     5401 agatgcccat tttgctcatc accttgagca tgcccttggc gaccgccttc tgatacgcgt
     5461 ggacgatgtc gttgtcgctg tacttcgcgg gcaacaggcc gtcccgctgg gcctgccaaa
     5521 gcgactcgaa cgccacgtac gggttgatcg catccgcgcc gtagccgacg agcaggcagt
     5581 ggtggtgtac ctcgcgggcc tcgcccgatt ccaggacgat cccgatccgc gtccgcttgg
     5641 cctgccggat caggtggtga tgggccgccc cgaccgccag cagcgagctg accggcaccc
     5701 gctcggccga aacgccgcgg tccgagagta cgatcaactg gtagccgtcg tcgatcgcct
     5761 tttccgcctc gcggcagatt cggtccagcc gtttgaccag ccccgcctcg ccctcggtgc
     5821 gggcaaacgt gatgtcgatc agcttgctct tccagccgcg atgttcgatg tggttgatcg
     5881 cggccagctc ttcgttcgtc agaatcgggt gcggcaacag caggcggtgg gcctgctcgg
     5941 gcgtcgtcgc cagcaggttc cgctccgggc cgacgtagca ctccagcgac atgatgactt
     6001 cctcgcggat cgaatcgatc ggcgggttcg tcacctgggc gaagagctgc ttgaaataat
     6061 cgtagagcat ccgcggctga tcgctgaggc aggccagggc cgaatcgttg cccatcgagc
     6121 cgacggggtc gcgcttctcg ccgaccagcg gcaacagcat gaactgcatc gtctcggtcg
     6181 aatagccgaa cgcctgcatg cgggccagca gcgtctccgg ctcaaagccg tgcgactggt
     6241 ccaccagagg caaatcgcgc agctcgacgc gctgctcctg cagccactgg gcatagggac
     6301 gcttggtcgc gacttccttc ttcacctcct cgtcggggat cagcgccccg cgctcgaaat
     6361 cgacgaggaa catgcggcca ggctgcaggc ggcccttggc cttcacgatc ttcggatcga
     6421 cgggcagcac gccgacctca ctggccatga tcacgcggtc gtcggtcgtc aggtaatagc
     6481 ggctgggccg gaggccgttg cggtcgagca cagccccgat atagcggccg tcgcagaaaa
     6541 cgaccgaggc cggcccgtcc cagggctcca tcaggcacga gtggtattcg taaaacgccc
     6601 gcttgtactc gggcatcgat tcgtggttct gccaggcctc ggggatcatc atcatcacgg
     6661 cctctggcag cgagcgcccg cccaagagca gcagttcgag cacgttgtcg aacacgcccg
     6721 aatcgctggt gctcaagtcg gtcaccggca gcagcttccg caggtccttg ccaaacagct
     6781 ccgattcgag cagcccctcg cgggcgctca tccagttgat gttgccgcgg agcgtgttga
     6841 tctcgccgtt gtgcgacatg aatcgcatcg gctgggcccg ctcccaagac ggaaacgtgt
     6901 tcgtgctgaa ccgcgagtgg atcatcgcca ggtggctggt gtaccgcgga tcggccaggt
     6961 cgggaaaata cggcacgacc tgatgcgacg tgagttgacc cttgtagatg atgatcctcg
     7021 agctgaagct ggcgacgtag tacatgttcc gctgtttcag cgcctgcttg ttcagagcgt
     7081 gaaacgcctg cttgcggatc aggtacagct gccggcaaaa ggccgtgcgc tcgatgctgt
     7141 cccccgccgc gacaatcaat tgctcgatga ccggctcggc tgcggccgcc gtcgcgccga
     7201 tatcggcgcc tttcacgtcg gtcggaactg gccgccagcc cacgagcttt tgtccctggg
     7261 ccgcgacgta tttctccagc gtgctcttgc aggttttgcg ctcggcatcg tcccgcggca
     7321 ggaacacctg ggccacggcg tagctgccga tcggcggcag ctcggaccca aacaatcgcc
     7381 tggcttccgc ccgcatcagc tcatcgggaa tcgccgtgag aattcccgcg ccgtcgccgg
     7441 tgttggcttc gcagccgcag gctccgcggt ggtccatggc gcgcagagcc gcgcaggcgt
     7501 cgcggacgat gctgtggctc cgcacgccct tgatgtgcgc gatgaagccc actccgcagc
     7561 tgtctttctc gaaagccggg tcgtacaggc cgtgcttttt cggcagtccg ttttcgagtt
     7621 ccagcatcat ggcgaaaatg ccttccccaa caaggatcgc cgcgaagcgc cagatagcag
     7681 cagcgcggcc tcttgactac gaagccaccg gcgccagcga ctcggctggt ttggacgcct
     7741 cggttttccc cggttccaag gcggtgctct ccgggccggc gatcccccgc gaatgaaccg
     7801 gctccttgag cagcaagtgc atgaaacggc tggccgtctt acccagctcg gttccgcggc
     7861 gctggatgat gccgatcggc cgcttcagtt cgcagccggc cagcggtcgg gccacgagcg
     7921 ccccggcggc gacctcgcgc tccaccgtcg gcaggggcag caggctgacg cccgcgtcga
     7981 tttcgacggc ccgcttgatc gtctcggtat tgtcgaactc catcatcacc cggacttcga
     8041 tcccgcgccc ccccagggcc cggtcgatct cgttgcggat ttccaggtcc gtgtcgaagg
     8101 ccacgatttc cagtccgtgc aggtcctcca gagtcacgcg gtcgcggagc gccaacttgt
     8161 ggcccggcga acagaccagc gccatcggct cctgccgcca gaccgtagct ttgatcgtgc
     8221 ggttcgagcg cggatagctg accaggccca gatcgacctg gttcgtttca accagctcgt
     8281 acacccgccg cggatgctga tactgcacgc gtacgttcgc cttgggatag gcctgcaaaa
     8341 accgctgcac aaagcgattc atgtgcgaca ggccgatcga atagatcgag gccacgctca
     8401 cccggccggc gacctcttca tgcaatgtgc ggacctcttc ttccagagcg aaatagcgct
     8461 gcaccaactt gcggcagccc tcgtaatacg cctcgccttc gggcgtaagc acgaacggcc
     8521 gcttcgtgcg gtcgatcagc ttgaccccca aatgctcctc caaaccatta acgatctggc
     8581 tcgctcccga ctgcgaaatg ccgttttcag acgcagcccg cgaaaagctg cgccggccta
     8641 caacgtcgca gaataccttg agcgacttga gatgcatgcc tgaattagcc ttggtaatac
     8701 tacgtcatcg aatgatgacc ggaaggcatc ataaagtagt cggctcagtg cgactcgtca
     8761 ataagcaggc tgaaaaattc gtgccgccag aataagcaat ccgcgaggtg gcaatctgag
     8821 ccgtgggaga aaacgcaggc aagaaattgc caaaaagctg ttgagcagcc cggcgctaat
     8881 ttaagagcga aggatctgtc tgagtggata gagtggccgt tcgctgggcc agccgttgtt
     8941 gggcccacgt tagccattcg ctcgccggca cgccgtacgg ccggaacttc tcggcgcggc
     9001 ccaactcctc gctgtagtag tacgccaggt gcgtgccgag cttgcttgcg gctgggctct
     9061 ccatcagctg gctcgagtcg gacgcgctca tttgcaggag cacccgctgg tcgatgtcgc
     9121 gcagcgattg ccggtcgagc caccggttgg cattgctgta ggaatcgcac cagatcagtg
     9181 cgtgcacgcc gaccgctggc ccttcgcgaa ggattgccgt caagtgtttg tcggcgcttg
     9241 ccgcggggcg gtcgtcgaac gacaagccaa agtcatccga ctttttcaat tcgcgaaacc
     9301 gcgccagatg gtggatgaac agaaagatcg cctcaccagg agcgtcgccc cggctcatcc
     9361 gccgctccac ttcctcagcc agttgttgca cggccgcgcc agcctcgcgc ggggcgatga
     9421 cgcgggccgc cgaagccagc ggaccgcaga gcttgttcca gaatccggcc gccggcgaat
     9481 cgggcggcgc gccgtcgagc acccacagga tccctccgtg ggctgccagg gccagcgcgc
     9541 cgttggcgag cattcccagc gcgagctctt cctgctggcc gacgaccagc aagtggctgc
     9601 ccgtttggcg gcggaatacg gcgctagtgg gatccttgat cgccacggcc gcgccgagcc
     9661 aggcccgcgg cgctcctgcc gccgttggct gtgccgcttg gagcacgtcg cgcaaagccg
     9721 cattcgccgc cggatcggcc gccatgtttc cctcgaagat tgtcggcgga tcgcatttca
     9781 atcctcgcag ctgcgctagt tccgcggcac gccgcagctg cgattcgcgc tcgtgatcac
     9841 tcaaccaaac cacctggaac ggatgattgc cctcgaccag gccgttggcg tcgttgtaga
     9901 tcgcctcgcc cggccggttg agcagccggg ccgcggtgtt gtcctcactg aggatcagat
     9961 gcgcatcgga ctcgctgcac tgcagagcga tccggaccgc catctgcccc agcgtgctgc
    10021 gggccagcga ataggccccg gcgagcgtct gcgaacccag cagcacatga atgccaaacg
    10081 cccggccctg ccgcaccagc cggtccagga gcagcgacgc atcgtgcgaa atcttgtcgt
    10141 cgctcgtgaa gaactcctga aactcgtcga tcacgagcag aatccgcggc agcacggcct
    10201 cgcggttggc gttgcggtag ccggccacgt cctgcacgcc cgcctgccgt aagaggtcgc
    10261 cgcggcgctt gagttccacg tccaatcgct ccaacacgct catgccgaac tcgcgttcac
    10321 tttcaattgc aattacgcgg gcgtgcggaa gttggtgcgt ggcgtaggcc ttgaactcta
    10381 cgcccttctt gaagtcgatc aaatagaagt gcagctcatt gggtgaatag tgagcggcga
    10441 ggttcgtaat gagcgcgttg agcagcgtcg atttgcccga gccggttttg cccgcgatca
    10501 gcacgtgctg cgacgtaccc cggcccagcc gcaggtactg cagctttttc gccccggccc
    10561 ggccgagcgg cacttcgatt ccgcggcggc tgtcggcttt ccaccactgg tccgccgccg
    10621 gcaaaacggc ctcgaacggc acctcaacac gcgccgtctc cttggcccgc tggccgacgg
    10681 cctgcaccag ctcggtgaac cgctcgtcgc cgggcggcga atcgagtacg agccgcagcc
    10741 ctcgcaagtc ggcgtcgcgc caggcaaacc gttgctcagc agcgtcccag acgagtgtga
    10801 ccgaatgccg ctccaagtcg gccaggtcga agttccgcgg gagagcgagc ttctggtcgg
    10861 tgctgatgag cgtgaagacg ccgcagcgag cgccgctggc ggcgatcgcg gccagccgcc
    10921 gggccgcttc gtccgaaaaa ttcgcgggaa agtttgcgac gaccagaatc tgatagggct
    10981 ccgcaacctc gcccgcgtcg gcgttgtagt cctggatcga ggcaaactcg ttccgcaggt
    11041 acttctggat cacgttctcc atgtgctccg tcaggtccgc cagccgctga ttgatgtggg
    11101 ccgactcggt ccagatgcgg ctggtcacca gccgcgggtc gaagtcggcc aggtgcatga
    11161 acgcagagaa attctggccc aatccgacgg gatcgatgat cgtgaagcgc accttgcctg
    11221 gcggaaacgt tgtcaggagc cgcagcatgg tgttttgcag cacgagcgcc gctgcgtcgc
    11281 gcgaagcgcc ctcggcctcg agcacgagtg aaggacgttg cggatagctc agtacgaccg
    11341 gcaaccgcac cgcggccggc gcctgctcaa gttgctctgc cgcgggcaat ccgccgggca
    11401 gctggtccag ccgcagctcg tggcggccga tgcacagcgc ggtcgctgca gcggcgacgg
    11461 cggcttgccg ctgcggctcg actgcatccc acgcgggaaa atgggcgtcg cagtcggcat
    11521 tcatggcgcg ggcggcggac tgaaaatcgg ccaggccggc gctccagcgc ttcgcgagcg
    11581 agttccactc tccggcaaag cgggcgtcgt tagccgtctg tagcgcgcgg tgctgctcgc
    11641 cgcggcgggc tgcatcctcg gcatagtcct gctctacgcg ggccagttgc ggcggatggc
    11701 gctcctcgag ctgctggaat tgccgctcgt aggtttcctc cagcgcttca cggcgcacgg
    11761 tgaacgcctg cgtcagctgc tcggtccgcg ccgcgtgctc ggccttgagc gcccggcggg
    11821 cttgcctcca ctcggccttt gcggccgcca ggtccagatc gcgctgctcg ccgaggtggt
    11881 cgtacttgcg ctccccctct aggtgcgccg cggcctgggc cgccgccagc tcctcgcggg
    11941 ccaaagccac cgccacttga aaatccggca actgctggag cgtttgccgg cgcgcaatcg
    12001 gccgggcgat ttgccgtgcc gccaaggcca ggcagagcgg gacgatcaga gccgcggcaa
    12061 ttacccagcc ccagccaaac gccgcgagca ggagccacgc ggggtaggcc accagcggaa
    12121 gcgccagcaa gaatatgagc actggccagc cgtcctccag gaaccgagcg gcccgctggt
    12181 gctggagggc atacaccagc gcgtatgccc gctgcatcgc ggccgtgaac cgctcgacgt
    12241 gctgcgccga cgcgcgttgc ggcgctgcgg ccgccggagg tggcggcggg tttgcatcgt
    12301 cgggccaagg gcagcggcgg cggacgatct tctgagcctg cgtgatcagc gactggattt
    12361 cggcttgccg gacctcgcac tgctgcttga actgggcgaa ctcgctgcgc gggcgctggc
    12421 tatcgagttt gtaggccgcg aggatcttct ggctctggtg ctgacagagc gtcttgcccg
    12481 cctcccgcgc cccctcgtac tcctggtcgg cggcagccaa ggcccgctct tcttccttag
    12541 cgagcttgaa gcccgccgcc tcgtactggc aaaacaccga ctcgcgggct gccttgtagg
    12601 ttctctccag cgccgcatgt ttcgcgtcga atcgcgaccg ctccagctgc tcgacctgct
    12661 cgtagtcgtg ctgagccacc gccgagagcg ccatttgccg ctgccgtatg tcg
//
LOCUS       DASRJB010000194         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3438107, whole genome shotgun sequence.
ACCESSION   DASRJB010000194 DASRJB010000000
VERSION     DASRJB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3438107"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1661
                     /gene="proS"
                     /locus_tag="VFB80_05410"
     CDS             144..1661
                     /gene="proS"
                     /locus_tag="VFB80_05410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZN33232.1"
                     /translation="MAKTAITPTRAEDYPEWYQQVVRAADLAESSDVRGCMVIKPWGY
                     SIWENMQRVLDGMFKATGHENAYFPLFIPLSFLQKEADHVEGFAKECAVVTHHRLVPN
                     SEGKLIPDPASQLEEPLIVRPTSETIIGATYARWVQSYRDLPILINQWANVVRWEMRT
                     RLFLRTTEFLWQEGHTAHATAEEAQEETMRMLGVYADFAENWIAMPVIQGEKTAGERF
                     PGAVATYTIEAMMQDRKALQGGTSHFLGQNFSKAQEIKFQDQNGTVQYAWTTSWGVTT
                     RLVGALTMTHSDDDGLVLPPRLAPRHVVLLPIYRSDEEKSQVLAYVNSLKSELEAQDF
                     VGGKVRVQIDDRDIRGGEKNWYHIKRGVPLRAEIGPKDIAKNGVFLARRDTGEKAGAL
                     RGEFVATIAQRLTAIQDNLLARAKQFREDNTRTIDTLDDFLAYFTPKSKNEDKAEIHG
                     GFALCHYAEGPELTELLKKHKVTIRCIPLGAANEPGKCFLTGQPSARRAVFAKAY"
     gene            complement(1658..3220)
                     /locus_tag="VFB80_05415"
     CDS             complement(1658..3220)
                     /locus_tag="VFB80_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN33233.1"
                     /translation="MTAARRLLLLPLAALAAAAGCFSDTSTAPPAVTTFPAAKTPQAE
                     QAAQPLAAKEPEPAPLFGDWQKPAVALVLSGQQLGYIEPCGCTGLENQKGGLARRHTL
                     IQQLSSERGWAVVPLDVGNQVKRFGKQQELKFTSTIKGLRTMGYRALAFGDNDLKLPA
                     GELLAALSNPDGKIPDVVSANVGLFDMDLQPRFLVLEAGGKQIGVTAVLGEKFEQKLA
                     GGDLVHQPPIKALKEFCRELDAKQCDLNVLLVHGSLEEAREIAAEVPVFDLVVAAGET
                     SLPSRELESVPGTKARLLQVGQKAMHAGVVGFYADEKKTFRYQSVPLDARFADSPAML
                     KLLAEYQDNLKTLGLNGLGLKPRPHPSGNKFVGSGKCGECHTKAFAKWSTTPHAHATD
                     SLVKPPNSRGDIPRHFDPECLSCHVTGWEPQQFAPLASGYLSLEQTPLLKHNGCENCH
                     GPGSAHVAAESGEGNVSAADAAKLRDAMKLPLAGAERRCLECHDDDNSPDFHKPGAFE
                     KYWKEVEHRGKD"
     gene            complement(3250..4410)
                     /locus_tag="VFB80_05420"
     CDS             complement(3250..4410)
                     /locus_tag="VFB80_05420"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HZN33234.1"
                     /translation="MKAALWAAAALVLGSAIGFFWTHFEFAHDDLPIAVTPVAGGTSL
                     APPGKVGPKAVVVNGERHDFGTMDRNAHEHHTFEVRNDGDEPLTLTTGQTTCKCTSFA
                     AGNNTVAPKQTVEVKLEWDAKTSEPQFEQSATLHTSDPRRREIHLSVHGRVIDTVRPE
                     SFDIHFSNASSNEAAKATVKIFAYRGNELIVQKHELLSADQAGWFNVSFEPLSADEVS
                     QQPDAKAGVLMTVELKSGLPIGRIDQQIRLTTNQNPDAPVTIRVYGDVASDVSLIGPG
                     HPEQLLIELPTISRDQGTRQTVHLMIKGPYRDQTELKIASVEPADEFTATLGEPNRDN
                     PKRVRYPLTIEVPPNARPVSRNSAGAFAQIRLTTTHPELKELTIRVRYVVPE"
     gene            complement(4407..>5519)
                     /locus_tag="VFB80_05425"
     CDS             complement(4407..>5519)
                     /locus_tag="VFB80_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33235.1"
                     /translation="LSRGLRQAPSASTDSGDEAKTARRTIFGGALAGLLLAMPLALAD
                     DSPLTIVPGLGSLPVAWLLGIPLLALCWWSFEPWIAGGRLLLAALIVPQVVLLVNLLA
                     AGALEFPGVVGTLLVLAPAALCYSRLSGEAQAAAGSINLPRWIPLSHNATPVATIAAI
                     LLTLLCLFTEYQPVLYSTGRLQLAKANWRTGTTEQAEAAAQKAAAADPRWDEPWRFLA
                     ELRLQRWLASGSEGDWQSFAAAADELRRRDPRHHLTYYARGNWYLTAWRKSGRPDLLE
                     QAIEEYRETSVRYPNWALYHAQLAWALHLAGREAEALSEAEIARRLDEATPHADRKLR
                     MQQIVDPQIEHGKTAEQVVAGLRNTVGSSTPQGKSP"
BASE COUNT          949 a         1783 c         1821 g          966 t
ORIGIN      
        1 gtggcggtca ggaatgtctg ccccacggtc gccctgcacg gcgaaagtgc gtttcgctaa
       61 aatccgccag tgagcgccgc gtggacgcgg cggctaagcg ggtctgccgc ctgaacctga
      121 accccgaacc ctgaaccttc ccaatggcca aaacggcaat cacccccact cgcgccgaag
      181 attacccgga gtggtaccag caggtcgttc gggcggccga cctggccgag agctccgacg
      241 ttcgcggctg catggtgatc aagccgtggg gctatagcat ttgggagaac atgcagcgag
      301 tgctcgacgg gatgttcaag gcgacggggc acgagaacgc ctattttccg ctgttcatcc
      361 cactctcgtt cctccagaag gaagccgacc acgtcgaggg ctttgccaag gaatgcgcgg
      421 tcgtcacgca tcatcgcctg gtgcccaaca gcgaaggcaa gctgatcccc gatccggcct
      481 cgcagctcga agagccgctg atcgtgcggc cgacgagcga gacgatcatc ggggcgactt
      541 acgcccgctg ggtccagtcg taccgcgacc tgccgatcct gatcaaccag tgggccaatg
      601 tggtccgctg ggagatgcgg acgcgattgt ttctgcggac gaccgagttc ctgtggcagg
      661 agggccacac ggcccacgcg accgcggagg aagcccagga agagacgatg cggatgctcg
      721 gcgtctatgc cgactttgcc gagaactgga tcgcgatgcc cgtgatccaa ggcgaaaaga
      781 ccgccggcga gcggtttccc ggcgccgtcg cgacttacac gatcgaagcc atgatgcagg
      841 accgcaaggc cctgcaaggc ggcacgtcgc acttcctcgg ccagaatttc agcaaggccc
      901 aggagatcaa gttccaggac cagaacggca cggtgcagta tgcctggacc acgtcatggg
      961 gtgtaaccac gcggctggtc ggggccctga cgatgacgca ctccgacgac gacggcctgg
     1021 tgctgccgcc gcggctagcg ccccggcacg tcgtgctgct gccgatctat cgcagcgacg
     1081 aagagaagtc gcaagtgctg gcttatgtga attcgctcaa gagcgagctg gaggcccagg
     1141 acttcgtggg cggcaaggtt cgcgtgcaga tcgacgaccg cgacatccgc ggcggcgaga
     1201 agaactggta ccacatcaag cgcggcgtgc cgctacgggc cgaaatcggc cccaaggaca
     1261 tcgccaagaa tggcgtcttc ctcgctcgcc gcgacacggg cgagaaagcc ggcgccctgc
     1321 gcggcgaatt cgtggcaacg atcgcccagc ggctgaccgc cattcaggac aacctgctgg
     1381 cgcgggccaa acaattccgc gaagacaata cccgcacgat cgacacgctg gacgatttcc
     1441 tggcctattt cacccccaag agcaaaaacg aagacaaggc cgaaatccac ggcggcttcg
     1501 ccctctgcca ctacgccgaa gggcccgagc tcaccgagtt gctcaagaag cacaaagtca
     1561 ccatccgctg catcccgctc ggagcagcga acgagcccgg caagtgcttc ctcaccggcc
     1621 agcccagcgc gaggcgggcg gtcttcgcga aggcgtatta atccttcccg cggtgctcca
     1681 cttccttcca atacttctcg aacgcgccgg gtttgtggaa gtcggggctg ttgtcgtcgt
     1741 cgtggcactc gaggcaccgt cgctcggcgc cggccagggg cagcttcatc gcgtcgcgga
     1801 gcttcgcggc atcggccgcg gaaacattgc cctcgccgct ttcggccgcg acgtgagcgc
     1861 tacccggtcc gtgacaattc tcgcaaccgt tgtgcttgag cagcggcgtc tgctcgaggc
     1921 tcagatagcc ggacgccagc ggggcaaact gctgcggctc ccagccggtg acgtggcagc
     1981 tcagacactc cgggtcgaag tgccgcggga tgtcgccgcg gctgttgggc ggtttcacca
     2041 gcgagtcggt ggcgtgggcg tgcggggtcg tggaccattt ggcaaaggcc ttcgtgtggc
     2101 attcaccgca cttgccgctg ccgacgaact tgttcccgct gggatgcgga cgaggtttca
     2161 ggcccaagcc gttcaggccc agcgttttca ggttgtcctg atactcggcg agcagcttga
     2221 gcatcgcggg cgaatcggcg aagcgggcat ccagcggcac gctctgatag cggaacgtct
     2281 tcttctcgtc ggcatagaat ccgacgacgc cggcgtgcat cgccttctga ccgacttgca
     2341 gcaggcgggc cttcgttccc ggaacggatt ccagctcgcg cgagggcagg ctcgtctcgc
     2401 ccgcggccac gacgaggtcg aacaccggca cttccgcggc gatctcgcgg gcttcttcca
     2461 gcgagccgtg caccagcagc acgttcaggt cgcactgttt ggcgtccagc tcgcggcaga
     2521 attccttcag agccttgatc ggcggctgat ggacgaggtc cccgccggcg agcttctgct
     2581 cgaacttctc gcccagcacc gccgtgacgc cgatctgctt gccgcccgcc tccagcacca
     2641 gaaaccgcgg ctgcaagtcc atgtcgaata gtccgacgtt ggcactgacg acgtcgggaa
     2701 tcttgccgtc cgggttcgag agcgccgcca acagctcgcc cgccggcagc ttgaggtcgt
     2761 tatcgccgaa ggccagcgcc cgatagccca tcgtccgcag gcccttgatt gtgctcgtga
     2821 atttcaactc ctgctgcttg ccgaaccgct tgacctgatt cccgacgtcg agcggcacca
     2881 cggcccagcc gcgctcgctg gaaagctgct ggatcagcgt gtgccgccgt gcgaggccgc
     2941 ccttctgatt ttccaggccc gtgcagccgc agggctcgat gtagcccagc tgttggccac
     3001 tcaagacgag ggccaccgcc ggtttttgcc agtcgccgaa gagtggtgcg ggctccggct
     3061 cttttgcggc cagcggctgc gcggcctgct ccgcctgtgg cgtttttgcg gcggggaagg
     3121 tggtgacggc cggtggcgca gtgctcgtgt ccgaaaagca gcccgcggcg gcggccaaag
     3181 cggccagcgg gaggaggagc aggcgtcggg cagcggtcat ggcttcatcc ttgaagggcg
     3241 cgacttggtt cattccggca cgacgtaccg cacgcggatc gtcagctctt tcaactccgg
     3301 gtgcgtcgtc gtgaggcgaa tctgagcgaa cgcgccggcc gagttgcggc tgaccggacg
     3361 ggcgttcggc gggacctcga tggtgagcgg atagcggacc cgcttgggat tgtcgcggtt
     3421 cggctcgccg agggtggcgg tgaactcgtc ggccggctcc accgaggcaa tcttgagctc
     3481 ggtctggtcg cggtaggggc ccttgatcat caggtgcacg gtttgccgcg tcccctggtc
     3541 gcgggaaatg gtcggcagct caatcagcag ctgctccgga tgtccggggc cgatgagcga
     3601 aacgtcgctg gccacgtcgc catagacgcg gatcgttacc ggtgcatcgg gattctgatt
     3661 cgtggtcaag cgaatctgct ggtcgatccg accgatgggc aggcccgact tcagctcgac
     3721 ggtcatgagc acgcccgcct tcgcatcggg ctgctggcta acctcgtcgg ccgaaagcgg
     3781 ctcgaacgac acgttgaacc agccggcctg gtcggccgag agcagctcgt gcttctggac
     3841 gatgagctcg ttgccgcggt aggcgaagat ctttaccgtg gccttggccg cttcattcga
     3901 cgaagcgttg gagaagtgga tgtcgaagct ttcggggcgg accgtgtcga tcactcggcc
     3961 gtgcaccgac aggtggattt ccctgcgccg cggatcgctg gtgtgcaacg tagccgactg
     4021 ctcgaattgc ggctcgctgg tcttggcgtc ccactcgagc ttgacttcga cggtctgctt
     4081 tggggctacg gtgttgttgc cggcagcgaa gctcgtgcac ttgcaggtcg tctgacccgt
     4141 cgtcagcgtg agcggctcgt cgccgtcgtt gcggacttcg aacgtgtggt gctcgtgggc
     4201 attgcggtcc atcgtgccaa aatcgtggcg ctcgccgttg accacgacgg ccttgggccc
     4261 gactttcccc ggcggtgcaa gcgacgttcc gccggcgacc ggcgtcaccg cgatcggcag
     4321 gtcgtcgtga gcgaattcga aatgcgtcca gaaaaagccg atggccgatc ccagcaccag
     4381 cgcggcagcg gcccagagtg ctgctttcat ggcgattttc cctgcggtgt cgagcttccc
     4441 acggtgttac gcagtcctgc gaccacctgt tcggccgttt tgccgtgctc gatttgcgga
     4501 tcgacgatct gttgcatgcg aagcttccgg tcggcatgcg gcgtggcttc gtccagccgc
     4561 cgggcgatct cggcctccga aagggcttcc gcctcccgcc cggcgaggtg gagcgcccac
     4621 gccaattgtg catgatacag ggcccagttg ggatagcgaa cgctggtttc ccggtactct
     4681 tcgattgcct gctccaggag atcgggccgg cccgactttc gccaggcggt caggtaccag
     4741 ttgcctcggg catagtaggt caggtgatgc cgcggatcgc gccggcggag ctcatcggcc
     4801 gcggccgcga acgactgcca gtccccttcg cttccactgg ccagccaacg ctgcaggcgt
     4861 aactcggcga ggaatcgcca gggctcatcc cagcgcggat cagccgcagc ggccttctga
     4921 gcggccgctt cggcttgctc agtcgtcccc gttcgccagt tggcttttgc gagctgcagc
     4981 cgccctgtcg aatacaacac cggctgatac tccgtgaaca ggcagagcag cgtcagcaga
     5041 atggcggcga tcgtggcgac aggcgtcgca ttgtgggaga gggggatcca gcgcggcaga
     5101 ttaatgctgc ccgctgccgc ttgggcttcg cccgacagcc gcgaatagca aagcgccgcc
     5161 ggcgcgagca cgagcagcgt tcccactacg ccgggaaact ccagtgcgcc cgcagccagc
     5221 agattcacca gcagcacgac ctgcggcacg attagcgcgg ccaacaggag ccggccgccg
     5281 gcgatccaag gctcgaacga ccaccagcag agcgcgagaa gtggaatgcc cagcagccag
     5341 gcgactggca acgagccgag gcccgggacg atggtgagcg gagagtcgtc ggcgagcgcc
     5401 agcggcatcg ccagcaaaag gccagcgagc gctccgccga agatcgtgcg gcgcgcggtc
     5461 tttgcctcgt cacctgaatc tgtcgaggcg gaaggagcct gccggaggcc ccgcgataa
//
LOCUS       DASRJB010000195         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2311413, whole genome shotgun sequence.
ACCESSION   DASRJB010000195 DASRJB010000000
VERSION     DASRJB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311413"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFB80_05430"
     CDS             complement(<1..931)
                     /locus_tag="VFB80_05430"
                     /inference="COORDINATES: protein motif:HMM:NF040416.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33236.1"
                     /translation="MGQEILYTSAPQGLRQGSSGFCTVGSTAGMEPNLAQFLEGFSGY
                     PHPYKPPDPRTKDNPVNYSFIQWTIGGQRYYVLSRVCDAGLDHTGRTNLLAHHVALTR
                     REIDRLPGGPAYVMAAGDFFITTWDKQLRTLAGQKPRGGDSPLACCEAWKRAAGDAGY
                     AGVLAEAAKEKGGRPVSVIYPAGTDVLALVAEAQSLLPPERRWNVTFATLYSRIYSSF
                     EVQWKFLFHETTEAKQARRDPHALVIDLCNGNLPQAQGGDLVTAARDGQFVTRAPQTI
                     AAREAPTPGRGRRPPVPSQVIAVEGDLTLAPPED"
     gene            complement(931..2298)
                     /locus_tag="VFB80_05435"
     CDS             complement(931..2298)
                     /locus_tag="VFB80_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33237.1"
                     /translation="MAGVSASVDLRAQVTCPHCWQNFPPDEVLWIAAHSDLMGDPMLP
                     SEPRRFLPTRFTVSGLAIDVKGAVCEHLACPNCHLHLPRAFLEMSPLFVSIFGSPFCG
                     KSNYLAAMTWQLKTTLRTSFRLDFANKNPLQNQFLGDNHTKLFNNPRQDELVLIDKTQ
                     QAGELYQPVLMKGQRILLPRPLVFSVSPDKGHPAFDAARLRNPLAKVLCLYDNAGEQF
                     LPGGESSSQPCKHLAVSKVRLFLFDPTQHPKFRLACQDKSKDPQFRQQKYLYSQDQVL
                     AEAASRIRSYSEPRLSQHEKIPGQLVVVVSKFDACQQLCRRKKLLETPVIRYSEKGSL
                     LDVDLLHAVSDEVRFLLTQYAGEVINEAESSFSDVVYIPVTALGRSPEQVDDQSNLFG
                     IRPRDVHSQWTEFPMLYALHRAAPKLVPVASHKIGVAKGTSKSNGDQRPGGEDAAEVR
                     LKETG"
     gene            2600..>2781
                     /locus_tag="VFB80_05440"
     CDS             2600..>2781
                     /locus_tag="VFB80_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33238.1"
                     /translation="MSSSDRLAALQSAIVRGEAAAIAGLIFPWDFTLIEDWEEGMAEE
                     NQGGMVVEVDGWLVAFT"
BASE COUNT          462 a          901 c          929 g          489 t
ORIGIN      
        1 aatcctccgg cggggcgagc gtcaggtcgc cttcgacggc gatgacctgc gacggcacag
       61 gaggcctcct gccgcggcct ggagttggcg cttcgcgggc cgcaatcgtc tgcggcgcgc
      121 gggtcacgaa ctgcccgtcg cgagcagccg tcaccaggtc gcccccttgc gcctgtggca
      181 gattcccatt acacaggtcg atcaccagcg cgtgcggatc gcggcgggcc tgcttcgcct
      241 ccgtcgtctc gtgaaaaaga aacttccact gcacttcgaa gctggaatag atccgcgaat
      301 agagcgtcgc gaaggtcacg ttccagcggc gttcgggcgg caagaggctc tgcgcctcgg
      361 ccaccagggc cagcacgtcc gttcccgccg gatagatcac gctgaccggc cggccccctt
      421 tttctttggc ggcctcggcc agcacgcccg catagccagc gtccccggca gctctcttcc
      481 aggcttcgca gcaggccagc ggcgagtcgc cgccgcgcgg cttttgcccg gcgagcgtcc
      541 gcagctgctt gtcccaggtc gtgataaaaa agtcgcccgc cgccatgacg taggccggac
      601 cgccgggcag gcggtcgatc tcgcgccgcg tcagggcgac gtggtgagcc agcaggttcg
      661 tgcggccggt gtggtcgagg ccggcgtcgc agacccgcga cagcacgtaa taccgctgcc
      721 cgccgatcgt ccattgaatg aacgagtaat tgaccgggtt gtctttcgtc cgcggatcgg
      781 gcggcttata ggggtgcgga tagccgctaa aaccctccag gaactgggcc aggttgggct
      841 ccatccccgc cgtgctgccg acggtgcaga agcccgacga cccctgccgc aggccctgcg
      901 gcgcggaggt atagagaatc tcctgcccca tcagccggtc tccttcaggc ggacctcggc
      961 tgcgtcttcg ccgcccggcc gctggtcgcc attcgatttg ctggtgccct tggcgacgcc
     1021 gatcttgtgg ctggccacgg ggacgagctt gggggcggcc ctatgcaggg cgtagagcat
     1081 ggggaactcg gtccactgcg aatgcacgtc gcgagggcgg atgccgaaca ggttcgactg
     1141 gtcatcgacc tgctccggcg agcggcccag agccgtgacc ggaatgtaca cgacgtccga
     1201 gaacgacgac tcggcctcgt tgatgacctc gccggcatat tgcgtgagca ggaagcgaac
     1261 ctcgtccgag accgcgtgga gtagatcgac gtcgagcagg ctgcctttct cgctgtagcg
     1321 gatcaccggc gtctcgagca gcttcttccg ccgacaaagc tgctggcagg cgtcgaactt
     1381 actgaccacg acgacgagct gtcccgggat cttctcgtgc tgcgagagcc gcggctcgct
     1441 gtagctgcgg attcggctag cggcctcggc cagcacctgg tcctggctgt agagatattt
     1501 ctgctggcgg aactgcgggt ccttcgactt gtcctggcag gccaggcgga atttcgggtg
     1561 ctgcgtcggg tcgaacagaa acagccggac tttcgacacg gccaggtgct tgcagggctg
     1621 gctcgacgac tcgccgccgg gcaggaattg ctcgccggcg ttgtcgtaca ggcagaggac
     1681 cttggccagc gggttgcgca gccgggcggc gtcgaacgcc gggtgcccct tgtccgggct
     1741 gaccgaaaag accagcggcc gcggcagcag gatccgctgc cccttcatca gcaccggctg
     1801 atagagctcg ccggcctgct gggtcttgtc gatcagcacc agctcatcct gccgcgggtt
     1861 gttgaacagc ttggtgtggt tgtcgccgag gaactgattc tgcagcgggt ttttgttggc
     1921 gaagtcgagg cgaaaactgg tgcggagcgt cgtcttcagc tgccaggtca ttgccgccag
     1981 gtagttcgac ttgccgcaga agggcgagcc gaagatcgag acgaacagcg gcgacatctc
     2041 caaaaaggcc cgcggcagat gcaggtggca gttcgggcag gccaggtgct cgcagaccgc
     2101 ccctttcacg tcgatcgcca gccccgagac ggtgaaccgc gtgggcaaga accgccgcgg
     2161 ctccgagggg agcatcgggt cgcccatcag gtcggaatgg gcggcgatcc agagcacttc
     2221 gtcgggcggg aagttctgcc agcaatgcgg gcaggtgact tgcgcccgca aatcgaccga
     2281 cgcactgaca cccgccatga acgcctgcct catcagataa gcccggcccg gcaatggccc
     2341 ccaaagggtt catcctaact ctataggcgt caaacggcaa cgaattccct ggattccccg
     2401 ctcgcatgcg gtgggcagcc gcgcagcgcc aaattgcgcc cgttcggggt ggggctcgtt
     2461 tcttatcaat tccccgtggg cccgcgccca cggctacgcg attacgaccg cattccgtgg
     2521 cggagaagcc gccatttgca tcgggcggat tgtgccgcta cgttgacggc gaaccgtgcc
     2581 aacgcccatt gtggtgccca tgtcctcgtc cgaccgactc gccgccctgc agtccgccat
     2641 cgtgcgcggc gaggcggcgg ccattgccgg cttgatcttc ccgtgggact tcaccctgat
     2701 cgaagactgg gaagaaggga tggccgagga gaaccaggga ggcatggtcg tcgaagtcga
     2761 tggctggcta gtggcgttca c
//
LOCUS       DASRJB010000196         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1466255, whole genome shotgun sequence.
ACCESSION   DASRJB010000196 DASRJB010000000
VERSION     DASRJB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466255"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFB80_05445"
     CDS             complement(<1..946)
                     /locus_tag="VFB80_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33239.1"
                     /translation="MPTKKVTREQRRGFLKQALAATGAAIAAPTIIPSSALGLDGAVA
                     PSERVIVGGIGIGNRGGYDLGCFLEQKDVQFVAVCDVREVRRLAVKKRVDEQYGNQSC
                     LMIRDFRELLDRPDIDAVLIATGPNWHATMSMQAAKAGKDMYCEKPCTKNIEQSLILR
                     DTMRRTGRVFQVGTQRRNLPHFAFACELARTGKLGKLQRIYAHPRGMLAITSGWFPAE
                     PEPAKNVVDWDMWLGPAAWRPYNAKLVDGFNFEKGGGMIGGFEFDAMGRKTIGGGVLE
                     WGSHCVDLCQWAVDDCPPPVEYNPPKDGQIVCKYQNGVE"
     gene            1435..4461
                     /locus_tag="VFB80_05450"
     CDS             1435..4461
                     /locus_tag="VFB80_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33240.1"
                     /translation="MYARSLIAVAVLLFIAPVAPGGDVAPLVSKIGTSRGVCVVLGDP
                     QGSLALELARATELTIFLQLQREQDVAAARKQVDAAGLLGTRVYVQKGSAAHINLADD
                     LADAVIVAETAAAMPREELLRVLRPAGKALVGETELVKPFAPGTDEWTHPYHGPDNNP
                     MSSDQVARRPYLTHFMVEPWYCPLSQMTVISGGRMFKVFGDRSSARPQEQLLNKLLAM
                     SAFNGTILWKRDLTPGFMVHRNTLAATPDTLFLADDKSCLLLDAATGQVRREIVVPEG
                     LSDGPVWKWLAVEGGVLYALVGASEKADEPLRGDRIRGAGWPWWKINEYEFGFGRTLL
                     AIDPATSRVLWSHRETDRIDGRALCLKGGRLYYYCDGKFLACLDAKSGASIWKNSSAD
                     LLEAIGPAGAAQNPLLGFASTAYMKCSDEGLFFAGPQRPRIVGASTQDGRLLWQHKGG
                     NAQLVLRPDALYALGEGRINSVTSSLKLEPLSGKVLAEFPSRDRCTRATGCFDSIFTR
                     GGAGGSTSVFDVTSSQPKLGVVTPMRPACTDGVVAAHGYLYWGPWMCRCDMTQLGVIS
                     LGHGGGFDYEAAATSAQRLETAANATQVATLSVTGDDWPALRKDNRRSTFTGQVTPQQ
                     VNQRWEFKPARPIMPTAPVAAAGLVLVSGGDGIVRALDAKTGQLRWSAYTGGGPIKYP
                     PAIEGGRAFVGSGDGWVYALEAASGRQLWRFRAAPAERMIPIYGSLTSTWPVGSGVLV
                     DQGVVYAASGISNFDGTHVYALDGASGQIRWQNHTSGSGGSELPEGGVSVQGHLLLHD
                     GAIYMHGGNQLATPERPKVAVQTSVAEFLAKRTERSVASYAVSDGTFAAKGTGRGKDL
                     FVRHGQVQATGFPLYWRPEDDHFLSTLELETPGGVIAVTTNPNLSQGSTLSRLAGAAE
                     GAEKPAALWTDKASQEIAAVAVTKNAVLVTGLNRSPQKPQQAAAAFYALELSTGTLLW
                     KHALPASPVAWGLAVDRTGQMIVTLTDGRVVCFAAD"
     gene            complement(4463..>4724)
                     /locus_tag="VFB80_05455"
     CDS             complement(4463..>4724)
                     /locus_tag="VFB80_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33241.1"
                     /translation="LTKDDCVGSHWCYGYRAHSFQTFFYNVINQDDGRNVGTPWIIDD
                     AFLFAATPPANKVRMANSTPPAASQPSTAQKPAAEQTKPAAR"
BASE COUNT          781 a         1566 c         1580 g          797 t
ORIGIN      
        1 gctccacccc gttttggtat ttgcagacga tttggccgtc cttcggcggg ttgtactcga
       61 ccggcggcgg gcagtcatcg acggcccact ggcacagatc gacgcagtgc gaaccccatt
      121 ccagcacgcc gccgccgatg gtcttgcggc ccatcgcatc gaattcaaag ccgccgatca
      181 tgccgccgcc cttctcgaag ttgaagccgt caaccagctt ggcgttgtac ggccgccagg
      241 cggcggggcc gagccacatg tcccaatcga cgacgttctt ggccggctcc ggctcggcgg
      301 gaaaccagcc gctggtgatc gccagcatcc cgcggggatg agcatagatc cgctggagct
      361 tgccgagctt gccggtgcgg gccagctcgc aagcgaacgc aaagtgcggg aggttccgcc
      421 gctgcgtgcc gacctggaac acgcggcccg tgcggcgcat cgtgtcgcgg aggatcaggc
      481 tctgctcgat gttcttcgtg cagggttttt cgcagtacat gtccttgccg gccttggccg
      541 cctgcatcga catggtggcg tgccagttcg gtcccgtggc gatcaggacc gcgtcgatgt
      601 cggggcggtc cagcagctcg cggaagtcgc ggatcatcag gcagctctgg ttgccgtact
      661 gctcgtccac ccgcttcttg acggccaggc ggcggacttc tctgacgtcg cagaccgcga
      721 cgaattgcac gtccttctgc tccaggaagc agcccaggtc gtagcctccc cgattgccga
      781 tgccgatgcc accgacgatg actcgctcgc tgggagccac ggcgccgtcc aggcccaggg
      841 ccgagctggg gatgatcgtc ggggcggcga tcgctgcccc ggttgccgcc agggcctgct
      901 tcaaaaaccc gcgccgctgc tctcgcgtga cctttttcgt gggcatgctg ctctcctagg
      961 caaatccgct tgttccagaa tgccgacagc atactccccg cggcaggcgc atttctacgg
     1021 cccagcgagg cagcacccgc agcaccgtga acagtcccta accagggggc gtgcgaaaac
     1081 gcctccgggg ctaatgccat cgttgcaagt tatgtccgat aaatgactta gcatcaattg
     1141 ccacaacatt actttagatt tgcctttgaa gccttagaga ccgtctgaac aggtcgcgaa
     1201 aggacgcttc gattggctcg tggagcgtca cgacggatct tcacgctcgc ggcggaatca
     1261 ggccgaggcg gcgcgcggcc gtccgccaaa atggcaggtg cgcgccaatc ggaactttac
     1321 tgcaaatagc gtaccaatgt tcacaactcg cccgaatggt ccggtgcggc gtatcacttt
     1381 gggcggctgc cggggccgca aatccaggct ctcagtgtga aggaggtgcc gatcatgtac
     1441 gctcgctcgc tcatcgcagt ggcggtactt ctcttcattg ctccggtggc ccctggcggc
     1501 gacgtagcgc cgctggtctc caagatcggc acgtcgcggg gcgtgtgcgt cgtgctgggc
     1561 gatccgcaag gcagcctggc gctcgaactg gcccgggcca cggagctgac gatcttcctg
     1621 caattgcagc gcgagcaaga cgtggctgcc gcccgcaagc aagtggacgc ggccggcctg
     1681 ttgggaacgc gagtctacgt gcaaaagggg agcgccgcgc acatcaacct ggccgacgat
     1741 ctggccgatg cggtgatcgt agccgagacg gctgccgcga tgccgcgcga ggagctgctg
     1801 cgcgtgctgc ggccggccgg caaggcgctc gtgggcgaga cggagctcgt caagcccttc
     1861 gccccgggca ccgatgagtg gactcacccg taccacggcc cggacaacaa cccgatgtcc
     1921 agcgaccagg tcgcccgtcg gccctacctg acgcatttca tggtcgagcc ctggtattgc
     1981 ccgctctcgc agatgaccgt gatctccggc gggcggatgt tcaaggtctt tggcgaccgc
     2041 tcgtcggccc gcccgcagga gcagctgctg aacaagctgc tggcgatgag cgctttcaac
     2101 ggcacgatcc tttggaagcg agacctgacg cccggattca tggttcaccg caacacgctc
     2161 gccgccacgc ccgatacgct cttcctggcc gacgataagt cgtgcctgct cctcgatgca
     2221 gccaccggcc aggtgcggcg ggagatcgtc gtgcccgaag ggctgtccga cggcccggtt
     2281 tggaagtggc tggccgtgga aggaggcgtc ctctacgccc tcgtcggcgc gagcgaaaag
     2341 gccgacgagc ccctgcgggg cgaccggatc cgcggcgcgg gctggccctg gtggaagatc
     2401 aacgaatacg agttcggctt cgggcggacg ctgctggcca tcgatccggc caccagccgc
     2461 gtgctgtgga gccatcgtga aaccgaccgc atcgacggcc gggccctctg cctgaaaggc
     2521 ggccgcctct actactactg cgacggcaag ttcctggcct gtttggacgc caagagcggc
     2581 gcatccatct ggaaaaactc ctcggcggac ttgctcgaag ccatcggccc ggccggcgcg
     2641 gcgcagaatc cgctgctcgg ttttgccagc acggcctaca tgaaatgcag cgacgagggc
     2701 ctgttcttcg ccggtcctca gcggccgcgg atcgtcggcg cgtccacgca agacggccgg
     2761 ctcctctggc agcacaaagg cggcaacgcg cagctcgtgc tgcggcccga cgctctctat
     2821 gcgctcggcg aggggcggat caactcggtc accagcagcc tcaagctgga gccgctctcg
     2881 ggcaaggttc tggccgagtt ccccagccgc gatcgctgca cgcgggccac cggctgcttc
     2941 gacagcattt tcacgcgtgg cggcgcgggg ggcagcacgt cggtcttcga cgtcaccagc
     3001 tcgcagccca agctgggcgt cgtgacgccg atgcggccgg cctgcaccga cggcgtcgtc
     3061 gcggcccacg gctatctcta ctggggaccc tggatgtgcc gctgcgacat gacgcagctg
     3121 ggcgtgatca gcctgggaca cggcggcgga ttcgactacg aggctgccgc gaccagcgcc
     3181 cagcggctgg aaacggcagc caatgcaacg caggttgcga ccttgagcgt cactggcgac
     3241 gactggcccg cgctgcgcaa ggacaatcgc cgcagcacgt tcaccggcca ggtcacgccg
     3301 cagcaagtca accagcgctg ggaattcaag ccggcgcgcc cgatcatgcc aacagcgccg
     3361 gtagctgccg cgggattggt tctcgtcagc ggcggcgacg gaatcgtgcg ggccttggat
     3421 gccaagaccg ggcagctccg ctggagcgcg tacacgggcg gcggcccgat caagtatccg
     3481 cccgccatcg agggcggccg ggcctttgtc ggctccggcg acggctgggt ctatgcgctg
     3541 gaggccgcca gcgggcggca gctgtggcgg ttccgcgccg cgccggcgga acggatgatc
     3601 ccgatttacg gctcgctgac ttcgacctgg cccgtcggca gcggagtgct ggtcgatcag
     3661 ggcgtggtct atgcggccag cggcatttcg aacttcgacg gcacccacgt ctatgccctg
     3721 gacggcgcga gcggccagat tcgctggcag aatcacactt ccggcagcgg cggcagcgag
     3781 ttgccggaag ggggcgtcag cgtgcagggg cacctgctgc tccacgacgg cgcgatctat
     3841 atgcacggcg gcaatcaact ggcgactccc gagcggccga aggtggccgt gcaaacgagc
     3901 gtcgcggagt ttctcgccaa acgcaccgag cgctcggtcg cgtcctacgc cgtgagcgat
     3961 ggcacattcg cggccaaagg cacgggccgc ggcaaggacc tcttcgtgcg ccacggccag
     4021 gtccaggcca cgggctttcc gctctattgg cgtcccgaag acgatcactt cttgagcacg
     4081 ctggaactcg agacgcccgg cggcgtcatc gcggtgacga ccaacccgaa cctgtcgcag
     4141 ggttcgacgc tgtcgcgact ggcaggcgca gcggaagggg cggaaaaacc cgcagccctc
     4201 tggaccgaca aggcgagcca ggaaatcgcc gccgtcgccg tgacgaagaa tgcggtgctg
     4261 gtgacgggac tcaatcgcag cccgcagaag ccgcaacagg ccgcggccgc cttctatgcc
     4321 ctcgaattgt ccaccggcac gcttctttgg aagcacgctc taccggcctc gcccgtcgcg
     4381 tggggcctgg ccgtggaccg caccggtcag atgatcgtca cactgaccga cggccgcgtc
     4441 gtttgctttg ccgccgattg acctaccggg cggccggctt ggtctgctca gccgctggct
     4501 tctgagcggt ggaaggttgc gaggcggcgg gcggcgtgct gttggccatg cggaccttgt
     4561 tcgccggcgg cgtcgcggcg aagaggaacg catcgtcgat gatccagggc gtgccgacgt
     4621 tgcggccgtc gtcctggttg atgacgttgt agaagaacgt ctggaagctg tgggcccggt
     4681 agccatagca ccaatggctg ccgacgcaat cgtccttggt caag
//
LOCUS       DASRJB010000197         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3721683, whole genome shotgun sequence.
ACCESSION   DASRJB010000197 DASRJB010000000
VERSION     DASRJB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721683"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /locus_tag="VFB80_05460"
     CDS             complement(<1..2168)
                     /locus_tag="VFB80_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN33242.1"
                     /translation="MTRPVSLAALLLFVPVLAGAADDPKGIDFFEKNIRPVLVAKCYQ
                     CHSASAKELKGELRLDTREGVRKGGESGLVIVPGKPDESPLLKAIMHEEGLEMPPKEK
                     LPDSAIANFAKWIEMGAPDPRKPNVTTVGGKINILEARKHWSFQPPKVAKPAAAKNAA
                     WPTSEIDRYVLAKLEQKNLTAVADADRRTLIRRAYFDLIGLPPTPEDVEAFVNDKDPK
                     AFEKVIDKLLAMPQFGERWGRHWLDVARYGESTSKERNIPYPMAWRYRDYVYDSVAAD
                     KPYDQFIREQIAGDLFPAKNDEQRNEMLTATGFLAIGPKSLNTRNAEQFRMDVADEQL
                     DVATRGVMALSVACARCHDHKFDPIPTADYYAMVGIFKSTDVLGGVKAGNNRLGYAGD
                     FVYLKGSEKKDSGPSAEDRQKLEELNAELASVRTELQQLRRAANAAAAEETAKPAGKA
                     AKNAKNAAKKAKQAAKKALEGLPERDQAKARKLQQRVQQLTGEIAALETKSNASGDAV
                     MGVRDSADPADCRINIRGEVADLGDLSPRGFVRVLTYPQSPDVNKEQSGRLQLAVWLT
                     SKENPLTARVMANRVWYHLFGRGIVATIDNFGALGEAPANQELLDYLAVRFMNQGWSV
                     KKLIREVMLSRTYQLSSDHSDADYAADPDNTLVWRMNRRRLEAEAIRDAMLYVGGNLD
                     LKRPTGSLTQTITGGEIGRQARTEGLKKEVTFRSAYLPIV"
BASE COUNT          401 a          796 c          827 g          560 t
ORIGIN      
        1 tggacgatcg gcagataggc gctgcggaac gtgacttcct tcttgaggcc ttcggtccgc
       61 gcttgccggc cgatctcgcc gccggtgatc gtctgcgtca gcgagccggt gggccgcttc
      121 aggtcgaggt tgccgccgac atagagcatc gcgtcgcgaa tcgcctccgc ctccaggcgg
      181 cggcgattca tccgccagac cagcgtgttg tccggatcgg cggcgtagtc ggcatcgctg
      241 tggtcgctcg acagctggta cgtgcggctg agcatcacct cgcggatcaa tttcttcacc
      301 gaccagccct gattcatgaa ccgcacggcg aggtagtcga gcagctcctg gttggcgggc
      361 gcttcgccca gcgcaccgaa gttatcgatc gtggcgacaa tcccgcggcc gaagaggtga
      421 taccagacgc ggttggccat cacgcgggcc gtcagcgggt tctctttgct ggtcagccag
      481 accgccagct gcagccggcc gctttgttcc ttgttcacgt cgggtgactg cgggtaggtc
      541 agcacgcgga cgaagccgcg gggcgagagg tcgcccaaat cggccacttc accgcgaatg
      601 ttgatgcggc agtcggccgg gtcggcgctg tcgcggacgc ccatgaccgc gtcgccgctg
      661 gcattggact tggtctccag cgcggcgatt tcgccggtca gctgctgcac gcgctgctgg
      721 agcttgcggg ccttcgcctg atcgcgctcc ggcaggcctt cgagggcttt tttggccgct
      781 tgtttcgcct tcttggccgc gttcttggca ttcttcgcag ccttaccggc gggtttggcg
      841 gtctcctcgg cagccgcggc attggcggcc cgccgtaact gctgcaactc ggtgcggacg
      901 ctggccagct cggcgttcag ctcttcgagc ttctggcggt cctccgcgct cgggcccgaa
      961 tctttcttct cgctgccttt gagatagacg aagtcgcccg cgtaacccag gcggttgttg
     1021 ccggccttca ccccacccaa gacgtcggtg ctcttgaaga tgccgaccat cgcgtagtag
     1081 tcggcggtcg gaatcggatc gaacttgtgg tcgtggcagc gggcacaggc gaccgagagg
     1141 gccatcacgc cgcgggtcgc cacgtcgagc tgctcgtcgg ccacgtccat gcggaactgc
     1201 tcggcgttcc gtgtgttcag gctcttgggc ccgattgcca gaaagcccgt cgccgtcagc
     1261 atctcgttcc gctgctcgtc gttcttcgcc ggaaacaagt cgccggcgat ctgctcccgg
     1321 atgaactggt cgtacggctt gtcggctgcg accgagtcgt agacgtagtc gcgataccgc
     1381 caggccatcg gatacggaat gttccgctct ttgctggtcg attcgccata gcgggccacg
     1441 tcgagccagt gccggcccca ccgctcaccg aactgcggca tcgcgagcag cttgtcgatc
     1501 actttctcaa aagccttcgg gtccttgtcg ttcacaaagg cctcgacatc ttcgggcgtc
     1561 gggggcaggc cgatcagatc gaaataggcc cggcggatga gggtgcggcg gtcggcgtcg
     1621 gccaccgctg tcaggttctt ctgctccagc ttcgccagca catagcggtc gatttcgctc
     1681 gtcggccagg cggcgttctt cgcggcggcc ggcttggcaa cctttggcgg ctggaacgac
     1741 cagtgcttgc gggcttcgag gatgttgatc ttgccgccga ccgtggtgac gttgggcttg
     1801 cgcggatcgg gcgcgcccat ctcgatccac ttggcgaaat tcgcaatcgc gctgtcgggc
     1861 agcttctcct tcggcggcat ctccaggcct tcttcgtgca tgatggcctt gagtagcggg
     1921 ctctcgtccg gcttgccggg aacgatcacc agccccgatt cgcctccctt gcggactccc
     1981 tcgcgggtat ccagccgcaa ctcgcccttc agctccttgg cgctggccga atggcactgg
     2041 tagcacttcg cgacgagcac cgggcggatg ttcttttcga agaagtcgat ccccttcggg
     2101 tcgtcggcgg cccctgccag gacgggaacg aacaggagca gagccgcaag agaaacgggg
     2161 cgagtcatcg aaagcctccg agcggagatg ccggcgcaat ttcagcctga caacctgatg
     2221 cgcgctgcgt tgtcgttatt gagtattcac ctgcggaatt caaccctgcc cgtcggcttt
     2281 cgggctgcgt gcatatgcat cttacggaga ttttgcgggg cggcgattct gacgtaaatg
     2341 tttatcctgt cgtggtttgc gctatgcagg tcgccttggc gttatgcagt ttatgcaggt
     2401 taaggctcgg tgcgtgcata aggcggcgga atgaaacaga aggaaacgaa ggaaacgaag
     2461 gggcagacgc accgtgtttg ctcatgcatg catattcgca agtattttcc aaagatggaa
     2521 ttgttgtaag ttatttattt ccaataggtt gcgacgaaca cgatgcgtcg cggctatgca
     2581 gttt
//
LOCUS       DASRJB010000198         8323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3721806, whole genome shotgun sequence.
ACCESSION   DASRJB010000198 DASRJB010000000
VERSION     DASRJB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721806"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1127)
                     /locus_tag="VFB80_05465"
     CDS             complement(117..1127)
                     /locus_tag="VFB80_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN33243.1"
                     /translation="MTDAGIELLAEPAILPFADSRYLRHRLGPGFIRQDVAFFSRTGR
                     CHAWHYLPESVDDAPLVVMAHGFGAEKAFRLPALAERFARRGMAVLLFDYRHFGQSDG
                     QPRQLICHRRQLEDWQSAVTYARTFLGTDLGRIALWGTSFGAGHALVTAARNPGLAAL
                     VLQVPMVDVPRCMGRDLRRVAQSLYHGLRDLCQRAVGLGPYEVPIVAPPGRFAVINEA
                     GCKAGYASLVPPGSRWQNACPASVLATAAFYRPIAVAHLVQCPTLVTVAASDQLIPPK
                     MIDRLVARLPSAALAKYPGDHFQLYHGEAFESLAAAQGDFLARQLLSEGSSPALAYGR
                     AA"
     gene            1324..3339
                     /locus_tag="VFB80_05470"
     CDS             1324..3339
                     /locus_tag="VFB80_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33244.1"
                     /translation="MPRRFRPLRYEPLEHRALPAGLPLVETIYLSTDTPGSATGSDGV
                     QVKFDDSDILRLDIERADTGELLAYQYRLIFDGSDVGLDSDDEDIDALAILADGSLVI
                     STTGRASVPGLTTEDEDLLRFTPAAGGLGYHTAGSWSLYFDGSDVGLALDSADVDALA
                     LLPDGRLVISTTGWFFVPGATGGGEDLLAFQPTSLGPNTAGKWSLLFDGSQFGLTTTG
                     EKLDAVDLTNTTALHLSTSGPFTVSGAAGDDEDAFSFDLASKTYQPGLRLDGSAVGLA
                     ALDIDALEIRADGEVLPEPNAALPTIDVAGVLAVAATKLAAHDAKNVIKTRYAGTAAL
                     TATSWTAVAPSDWTAGFYPGLLWQMYERTGDVVWRNKAVAWSAGLESQKTRTSTHDLG
                     FMLFNSFGQGLRLTGDPHYQEVVLEAARSLSSRYNPTVGAIRSWDGTGYRVIIDNLMN
                     LELLFHAAKNGGTTWSGGPAQDLYNMAVSHASKTLQNHVRPDGSTYHVVTYDAATGGV
                     LSKSTAQGKSAESTWSRGQAWAIYGFTMTFRETGDATFLEAARRTADYFLANLPDDFV
                     PPADFSSTYTGLAYKDSSAAAVAASGLLELSTLETDSARGERYFQSAVMILESLTSAS
                     YVAPPQHPALLLHGARTYPGQNISLIYGDYYLVEALLRYGRIVNPRS"
     gene            3383..3979
                     /locus_tag="VFB80_05475"
     CDS             3383..3979
                     /locus_tag="VFB80_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33245.1"
                     /translation="MTRSTVTMPWEILDRMERAVAKVRDRLLRSTAALNQAGIPYAVV
                     GGHAVASWVATVDEGAVRNTRDVDLLIRRENLPAVTAAIERAGFLPEKSFGVTMFLDG
                     PSAKPSESIHLLFAGEQVRENDPLPCPDLETIDDPAGFRVTKLSSLVRIMLLSNRNED
                     GMHIRDLIGVGLIDQSWPAKLPPVLAERLQAILDTPDA"
     gene            4103..7354
                     /locus_tag="VFB80_05480"
     CDS             4103..7354
                     /locus_tag="VFB80_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN33246.1"
                     /translation="MLRPPALLCLAAGVLLAVGQHLPAADGAGEEFFEKQVRPILVSR
                     CFECHSAKSVKLQGGLRLDSRAGVLKGGETGPAIVPGKPKESLLVDAINYGALYQMPP
                     KTKLPPDDIVVLTKWVEMGAPWPADPPVTAAGAVTKAFDLQKRQAEHWCWQPIQEPAV
                     PAIPNAKSPIPNPVDAYILEKLQSRGLSPAPPADRHTLLRRVYFDLIGMPPSPAELEE
                     FLSLAPSLLRSVSPSARDGAKEGQSDGEKERKAYVAVVDRLLDSPHFGERWARHWLDL
                     VRYAESRGHEFDYNIPNAFEYRDYVIRALNADVPYDQFVIEHVAGDLLEKPRLHPTER
                     FNESIVGTGFWFLGEWCHSPVDVRKDECDRQDNMLDVFSKTFLGLTVACARCHDHKFD
                     AISQQDYYALAGFLQSSSYRLTRFGTWEQDRRIAAELAALDAKHRPLIAAAIAKALEP
                     RIDELLKPLFERAANWGRDLGVPEVDLDGAEVIVDYSRPPAGAWMTDGPTWGSGPLKA
                     GELVLTGEVARPLEVMPYGAACRDWNWGELRVAAPHEGDAGRLGGWVRAGRTLKTPTF
                     TIDSGPVHYLIEGVGQVYAAVASHAMINGPLHGELQKETGGNSDLPTRWITHDLSRYA
                     GHRCHLEFSPKEGEDFRVLMVVQVRRPPAPVNRNSAIATALSDNPRQAAAATLKLLAA
                     DRIAAAEHPRDHAVFANWLIAAAKLDENSPPELEAAIAAYRADREKLIEQIQPAGRLC
                     LAMWDGTPADEHLLIRGNHKTPGPAVPRRLLEAMASGGRQPPDAVTSGISSQEPARGR
                     LELARQLVDPGNPLVSRVIVNRVWHHLFGRGIVPSVDNFGVLGQKPTHPELLDHLAGQ
                     FMQDGWSLKRLIRSIVLSETYRQSSGVRSRESAVDPENLLFHRQNLRRLEGESLRDAI
                     LAVSGRLDRQRFGPSVPVHLTPFMQGRGRPGESGPLDGAGRRSIYTAVRRNFLPPMML
                     AFDTPIPFSTIGRRNVSNVPAQALILMNDPFVAEQARHWARGLLAEKDATIEGRIKRM
                     YLATFSREPDTAEIAAARGFLEQQGRDYGAIDERAWTDLAHVLFNVKEFVLVE"
     gene            7362..>8323
                     /locus_tag="VFB80_05485"
     CDS             7362..>8323
                     /locus_tag="VFB80_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33247.1"
                     /translation="MFPCHRLTAPPTSRRDFLQQAGCGFGAVALAALWASRDLEGADI
                     KGNDPLAPRQPHFKPRAKSVIYLYMDGGPSQVDTFDPKPRLNQEHGRPFGLKMEPTQF
                     NNNGNTFGCPWKFKPYGQSGIPVSDLFPHVAQHVDKLAVIRSMTSQFPEHTSANYFLH
                     TGNGMQGRPSMGAWFGYGLGSECRDLPGFIVINGGLIPPGGLDNFNSGFLPASYQGSI
                     FTADNPPVANIVPREASGTLQRNKLALLKQLDAQVAERLGRVDAVESAIANYELAYRM
                     QTAVPELTEIKDETEATRRMYGLDAKSKGTASFARQCLLARRLI"
BASE COUNT         1500 a         2786 c         2660 g         1377 t
ORIGIN      
        1 gagcgacaag tacgcccagc ggttcgaaga gctgcagatg acgctgggaa tggattgcgg
       61 gtactaacgg ctaaagccgt tgcttcggcg acggtagttt ggccaggccc gccccctcat
      121 gcggcacgtc cgtaggccag cgcgggcgat gaaccttccg acaacaactg gcgggccagg
      181 aaatccccct gcgccgctgc gagactctca aacgcctcgc cgtggtagag ctgaaagtgg
      241 tctcccgggt atttcgccag cgctgcactg ggcagcctgg cgacgagcct gtcgatcatc
      301 ttgggcggga tgagctggtc gctcgccgca accgtgacga gcgtggggca ctgaaccagg
      361 tgcgccacgg caatcggccg gtagaaggca gccgtcgcca acacgctggc cggacaggca
      421 ttttgccatc tgctgccggg cggcaccagc gatgcatagc ccgccttgca gcctgcctcg
      481 ttgattacgg cgaaacgccc tggcggagcg acgatcggca cctcgtaggg cccaagcccg
      541 accgcccgct ggcagaggtc gcgcaggccg tgatagagcg actgagctac gcggcgcaaa
      601 tcgcgcccca tgcagcgcgg cacatcaacc atcggcactt gcagcaccag tgccgccagg
      661 ccagggttgc gggccgcggt gaccagcgcg tgccccgcgc caaacgacgt cccccacaag
      721 gcgatccgtc ccagatcggt ccccagaaag gtccgcgcgt aagtcaccgc ggactgccag
      781 tcctccagct gccgccggtg gcaaatgagc tgccgcggct gtccgtcgct ctggccgaaa
      841 tgccggtagt cgaacagcag cacggccatt ccccgccggg caaatcgctc ggccagcgcc
      901 ggcaggcgga aggccttttc cgccccgaag ccatgggcca tcaccacgag cggagcatcg
      961 tccaccgact cgggtaggta atgccaggcg tggcaccggc cagtgcggct gaagaaggcc
     1021 acatcctggc ggatgaatcc cgggccaagc cggtggcgca agtaccgcga atcggcgaag
     1081 ggcagaatcg ccggctctgc aagcagttcg atgccggcgt ccgtcatgac atcaccctgc
     1141 gataagactg gatcgcccgg ctcctctggc gttatcttcg gctagcagcg cggaaaagtt
     1201 gacgcgatct gtgcagtcgt catagtctcc ggcttggcgg acgcttccag aggacagcga
     1261 gcgagccgcc gattatgctc gctactgcag ctgtgaagtt catccttgcc gaccgcgccg
     1321 cgcatgcctc gtcgctttcg cccgctgaga tatgagcctc tagagcaccg cgcgctgccc
     1381 gccgggctgc cgctcgtcga gacgatctac ctttccacgg atacgccggg cagcgccacc
     1441 ggcagcgacg gcgtgcaggt gaagttcgac gattcggaca tcctccggtt ggatatcgag
     1501 cgggcggaca ctggcgaact attggcctac caataccggc tgattttcga tggcagcgat
     1561 gtcggactgg acagcgacga cgaagacatc gatgccctgg caatcctcgc cgacggcagc
     1621 ctcgtgattt cgacgaccgg gcgggccagc gtgcctggtc tgaccacgga ggacgaggac
     1681 ctgctgcggt tcacgccagc cgccggcggc ctgggctatc acacggccgg cagctggtcg
     1741 ctctatttcg acggcagtga cgtcgggctg gcgctcgatt cggccgacgt cgatgcgctg
     1801 gccctgttgc ccgatggccg gctcgtgatt tcgaccacgg gctggttctt cgttcccggg
     1861 gccacgggcg gcggcgaaga cctgctcgcc tttcagccca cgagcctcgg tcccaacaca
     1921 gccggcaagt ggtcgctgct cttcgacggc agccaatttg gccttacgac gaccggcgaa
     1981 aaactcgacg ccgtggatct gaccaatact acagcgctgc atctctccac cagcggcccg
     2041 ttcaccgtca gcggcgcggc cggcgacgac gaggatgcgt tcagcttcga cctggcgagc
     2101 aagacctatc agccgggatt gcggctggac ggcagcgctg tgggtctggc ggcgctcgac
     2161 atcgacgcgc tggaaatccg tgcagacggc gaagtcctgc cggagcccaa tgcggcactg
     2221 cccacaatcg acgtagccgg cgtcctggcg gttgcggcga caaagcttgc tgctcacgac
     2281 gccaaaaacg tcatcaagac gaggtacgcc ggcacggctg cactcacggc gacaagttgg
     2341 accgcggtcg cgccgtccga ttggacggcg ggtttctatc ccgggctgct ctggcaaatg
     2401 tacgagcgga ccggcgacgt cgtgtggcga aacaaagccg tcgcctggag tgccggcctg
     2461 gaaagtcaga agacgcgcac gagcacgcac gacctcggct tcatgctctt taacagcttt
     2521 ggccaaggcc tgcggctgac aggcgatcca cactaccagg aggtcgtgct cgaggcggcc
     2581 cgctcgctga gcagccgcta taacccgacc gtcggcgcaa ttcgctcgtg ggacggcacg
     2641 ggctaccgcg tgatcatcga caatttgatg aacctcgagc tgctgttcca cgcggccaag
     2701 aatggcggaa cgacctggtc gggcggtcct gcgcaggacc tctacaacat ggcagtcagc
     2761 cacgccagca agacgctgca aaaccacgtc cgccccgacg gcagcacgta tcacgtcgtg
     2821 acttacgacg cggcgaccgg cggcgtgctc tccaaatcga ccgcccaggg caagagcgcc
     2881 gagtcgacgt ggtcgcgcgg gcaagcctgg gccatttatg gcttcacgat gacgtttcgc
     2941 gaaaccggcg atgcgacgtt cctcgaagcc gcgcggcgga cggccgacta tttcctggcg
     3001 aacctgccgg acgatttcgt cccgccggcc gacttcagct ctacctacac gggcctcgcc
     3061 tataaagact cgtcggcggc tgccgtcgca gcttcggggc tcttggagct ctctacgctc
     3121 gagaccgatt ccgcccgcgg cgagcgctat tttcagtctg cagtgatgat cctcgagtcg
     3181 ctgacttccg cttcgtacgt cgctccgccg cagcatcccg cgctactgct gcacggcgca
     3241 cggacctatc ccggccagaa catcagcctg atttacggcg actattacct ggtcgaagcc
     3301 ctcctgcgct acggccgcat cgtgaacccg cgaagctaac ttgttattgg cagctgttgc
     3361 cgaatcaact tacgatgggt gtatgacccg ttcgactgtt acgatgccgt gggaaatcct
     3421 ggaccggatg gagcgagccg tggccaaagt tcgcgatcgg ctgttgcgct cgacggcggc
     3481 cctgaaccag gccggcatcc cgtacgcggt cgtgggtggg cacgccgtgg cttcgtgggt
     3541 cgcaaccgtc gatgaaggcg ccgtgcgtaa cacgcgcgac gtcgatctgc tgatccgccg
     3601 cgaaaacttg ccggccgtga ccgccgcgat agagcgggcc ggatttctcc cggaaaagtc
     3661 gttcggcgtg acgatgtttc tcgacggccc aagcgccaag ccgagcgagt cgatccactt
     3721 gctgtttgcc ggcgagcagg tccgcgaaaa cgatcccctg ccctgccccg atctggagac
     3781 aatcgacgac cccgcgggtt ttcgagtcac caagctgagt tcgcttgtca ggatcatgct
     3841 gttaagtaat cgcaatgagg atggtatgca tattcgcgac ctcatcggag tcgggctgat
     3901 cgaccaaagc tggcccgcga agctccctcc cgtgctggcc gagcggctgc aagcgatcct
     3961 tgacacgccc gacgcgtgac gccctccgcc aaagacgcgc cggttttgac cgcattcgcg
     4021 gccggggcta gactgaaaag cgttcgccgc cccgcctttc ggcgcgatga gtgccgccag
     4081 ccacgctagg aaactcgcga gcatgctccg cccccctgcc ctgctctgcc ttgcggccgg
     4141 tgtgctgctt gcggttggcc agcacctgcc ggcggcggac ggcgcaggcg aggaattctt
     4201 cgaaaagcag gtgcggccaa tactggtttc gcgctgcttc gaatgccata gcgccaagag
     4261 cgtgaagctg cagggcggct tgcggctcga ttcgcgggcc ggcgtgctca agggcggcga
     4321 aacggggccg gccatcgtgc cgggcaagcc gaaagagagc ctgctggtcg atgcgatcaa
     4381 ctacggcgcg ctgtatcaga tgccgccgaa gacgaagctg cccccggacg acatcgtggt
     4441 gctgacgaag tgggttgaga tgggcgcgcc ctggccggcc gatcccccgg tgaccgcggc
     4501 tggggcagtg accaaggcgt tcgacctgca aaaacgccag gccgaacact ggtgctggca
     4561 gccgatccaa gagccggcag tgcctgccat cccaaatgcc aagtcaccaa ttccaaatcc
     4621 cgtcgatgcc tatatcctgg agaagctcca gtcccgcggc ctctcgcctg ccccacccgc
     4681 cgaccggcac acgctcctgc ggcgcgtgta cttcgacctg atcggcatgc cgccgtcgcc
     4741 tgccgagctg gaggagtttc tgtccctcgc tccctctctc cttcgctccg tctctccttc
     4801 agcgcgggac ggagcgaagg agggacaaag tgacggagag aaagagagaa aagcctacgt
     4861 ggccgtcgtg gatcgtctcc tcgactcgcc ccacttcggc gagcgctggg cccggcactg
     4921 gctcgacctg gtgcggtatg ccgaatcgcg cgggcacgag ttcgactaca acatccccaa
     4981 cgccttcgaa taccgcgact acgtcatccg ggccctcaac gcggatgtgc cttacgatca
     5041 attcgtcatc gagcacgtcg ccggggacct tttggaaaag ccgcggctgc atcccaccga
     5101 gcggttcaac gagtcgatcg tcggcacggg cttctggttc cttggcgagt ggtgccattc
     5161 gccggtcgat gtccgcaagg acgagtgcga ccggcaggac aatatgctcg acgtcttcag
     5221 caagacgttt ctggggctaa ccgtcgcctg cgcccgctgc cacgaccaca agttcgacgc
     5281 catcagccag caggactatt acgccctggc cggctttctg caaagctcgt cgtatcgcct
     5341 gacgcggttc ggcacctggg agcaggaccg ccggattgcc gccgagctgg cggcgctgga
     5401 cgccaaacac cgcccgctga tcgccgccgc aatcgccaaa gccctcgagc cccggatcga
     5461 cgagctgctg aagccgctct tcgagcgcgc cgcaaattgg ggccgggacc tcggcgttcc
     5521 ggaagtggat ctggacggcg cggaggtgat cgtcgattac tcacggccgc cggccggcgc
     5581 gtggatgacc gacgggccga cctggggcag cgggccgctc aaagcgggcg agctggtgct
     5641 gactggcgag gtggcccggc cactggaagt gatgccgtat ggcgcggctt gccgcgactg
     5701 gaactggggc gaactgcgcg tcgccgcgcc gcacgaaggc gacgccgggc ggctcggcgg
     5761 ctgggtgcgg gccgggcgga cgcttaagac gccgaccttt acgatcgatt ccggccccgt
     5821 gcattacctg atcgaaggcg tcggccaggt ttacgccgcc gtcgcttcgc acgcgatgat
     5881 caacggcccg ctgcacggcg aattgcagaa ggagaccggc ggcaacagcg acctgccgac
     5941 ccgctggatc acacacgact tatctcgcta cgcgggacac cgctgccact tggagtttag
     6001 ccccaaggaa ggtgaagact tccgtgtcct gatggtcgtg caagtccggc gaccgcccgc
     6061 gcccgtcaac cgcaacagcg cgattgccac ggcattgtcc gataatcccc gccaggccgc
     6121 cgctgcgacc ttgaagctgc tcgccgccga caggatcgcg gcagccgagc acccgcgcga
     6181 ccacgcggtt ttcgcgaatt ggctgatcgc cgccgctaag ctcgacgaga actcgccacc
     6241 ggagctggaa gccgcaattg cagcctatcg ggccgaccgc gagaagctta tcgagcagat
     6301 tcagccagca ggtcggctct gcctggccat gtgggacggc acgcccgccg acgagcacct
     6361 gctgatccgc ggcaaccaca agacgccggg accagcggtg ccgcggcgac tcttggaggc
     6421 gatggcgagc ggggggcgtc agccccccga tgccgtaacg tcaggaatca gcagccagga
     6481 gccggcaaga gggcgcttgg aactggccag gcagcttgtc gatcccggca atcccttggt
     6541 tagccgcgtg atcgtcaacc gcgtctggca tcacctgttt ggccgcggga tcgtgcccag
     6601 cgtcgataac tttggcgtcc tcggccagaa gcccacgcat ccggagctgc tcgaccactt
     6661 ggctgggcaa ttcatgcagg acggctggtc gctgaagcgg ctgatccggt cgatcgtgct
     6721 ctcggaaact tatcggcagt cgtcgggggt caggagtcgg gagtcggcgg tcgatccgga
     6781 gaatctgctg ttccatcgac agaacctgcg ccggctcgaa ggggagtcac tgcgcgacgc
     6841 gatccttgcc gtcagcggcc ggctggatcg gcaacggttt gggccgagcg tgccggttca
     6901 tctgacgccg ttcatgcaag gccgcggccg tccgggcgaa agcggtccgc tcgacggcgc
     6961 gggccgccgc agcatctaca cagccgtccg ccgcaatttt ctgccgccga tgatgctcgc
     7021 cttcgacacg ccgattccct tcagcacgat cggccgccgc aacgtcagca acgtcccggc
     7081 ccaggccctg atcctgatga acgatccctt cgtcgccgaa caggctcgcc actgggcccg
     7141 ggggctgctg gccgagaagg acgccacgat cgaagggcga atcaagcgga tgtacctggc
     7201 gactttcagc cgggagcccg acactgcaga aatcgctgcc gcgcgaggtt ttttggagca
     7261 gcaaggcagg gattacgggg caattgacga gcgcgcttgg acggatctgg cccacgttct
     7321 cttcaacgtc aaggaattcg tactcgtcga ataagatccg catgttcccc tgccaccgcc
     7381 ttaccgcccc gcccaccagc cgccgcgact tcctgcagca ggccggctgc ggctttggcg
     7441 cagtagcgct ggcggcgctg tgggcatcgc gcgacctcga aggggcggac atcaagggca
     7501 acgatccgct cgcgccgcgg cagccgcatt tcaagccgcg ggccaaaagc gtcatctacc
     7561 tgtacatgga cggcggcccg agccaggtcg atacattcga ccccaagccg cggctgaacc
     7621 aggagcacgg ccggccgttc ggcctcaaga tggagccgac gcagttcaac aacaacggca
     7681 acacgttcgg ctgcccctgg aaattcaagc cgtacggcca aagcggcatt cctgtcagcg
     7741 acctgtttcc gcacgtcgcc cagcacgtcg ataagctggc ggtcattcgc tcgatgacca
     7801 gccagtttcc cgaacacacg tcggccaatt acttcctgca caccggcaat ggcatgcagg
     7861 ggcggccgag catgggggcc tggttcggct acgggctggg gagcgagtgc cgcgacctgc
     7921 cgggcttcat cgtcatcaac ggcggtctga ttccacccgg cggcctcgac aatttcaaca
     7981 gcggctttct tccggccagc taccagggct cgatcttcac cgccgacaat ccgccggtcg
     8041 ccaacatcgt cccgcgcgaa gcgagtggca ccctccagcg caacaagctc gcgctgctca
     8101 agcaactcga cgctcaggtc gccgagcggc tgggccgcgt cgatgccgtc gagtcggcga
     8161 tcgccaacta cgagctggcc taccggatgc agacggccgt gcccgagctg actgagataa
     8221 aggacgagac cgaagccacg cggcggatgt acgggctgga cgcgaaatcc aaggggaccg
     8281 cgtcgttcgc ccggcagtgc ctgctggccc gccgcctgat cga
//
LOCUS       DASRJB010000199         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3158372, whole genome shotgun sequence.
ACCESSION   DASRJB010000199 DASRJB010000000
VERSION     DASRJB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158372"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFB80_05490"
     CDS             complement(<1..428)
                     /locus_tag="VFB80_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN33248.1"
                     /translation="MHLLLPLLALLAADARPNIVFVLVDDMGYADLGCMGAKDIRTPH
                     IDRLASEGLKFTDFYANAPVCTPTRTAFITGRWQQRLGFEWAMGFTAEQYRRVNGQLV
                     EEPDKLAFGLPQGETTIAELLKNQGYATGAFGKWHLGYQP"
     gene            complement(777..>2149)
                     /locus_tag="VFB80_05495"
     CDS             complement(777..>2149)
                     /locus_tag="VFB80_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN33249.1"
                     /translation="AATLCLAGPAFARLPNILLLIADDQRPDTIRALGNSHIQTPHLD
                     ELVRRGTVFTRATCAHPLCYPSRAELLTGCTGFRSGTYSELKLNEGVPLLPRLLKAAG
                     YRTWYVGKWHTAGRPSTVGYDECDGLYAGGRVGEEPYVDFRGRPATGYRGWQFQTDAG
                     QRFPEKGTGLTPNISAEFADAAIRLISQKSEQPFFVHVNFTAPHDPRIWPPGYEKTYD
                     AAKLPLPANFRGEHPFDHGNARGRDEVLLPFPRTEGDLRQELACYYAVISHLDAQLGR
                     ILKALDETGQRASTLIVFTADHGLAIGSHGLVGKQNMYEHTINVPFVIAGPGIPAGEK
                     RAAQCYLRDLFPTFCDLAGLATPPMDGRSLAPVLRGEKAEVHPFIVGYFQDSQRMIRE
                     GDWKLAWYPKIDRWQLFNVGSDPHELRDLIADLAQRDRVADLRAKLLAWLREQGDTSA
                     RESKEK"
BASE COUNT          360 a          683 c          712 g          394 t
ORIGIN      
        1 tccggctgat agcccaagtg ccacttgccg aatgcgccgg tggcgtagcc ttggtttttg
       61 agcagctcgg cgatcgtcgt ctcgccctgt ggcaacccga acgccagctt gtccggctct
      121 tccaccagtt ggccattcac gcggcggtac tgctcggccg taaaacccat cgcccactcg
      181 aagccgaggc gctgctgcca gcggcccgtg atgaaagccg tccgcgtcgg cgtgcagacc
      241 ggagcattgg catagaagtc ggtgaacttc agcccttcgc tcgccagccg gtcgatatgc
      301 ggcgtgcgaa tatccttggc ccccatgcag cccaggtcgg catagcccat gtcatcgacc
      361 aggacgaaga cgatgttcgg ccgggcgtcg gcggccagca aggcgagcag tggcaggagg
      421 aggtgcatga tgtgcttttc gttgccagga tttcgggcgt cggggctgcg tggtcaggtg
      481 taatcgcggc agcaattatc agccccctgg ttgggcaggg gcgaacgcgc cttttttcgc
      541 aattcttcgt cgcaaccctc gaatcgccag cgacttacgg cacgagcagc tgcgggccgt
      601 taaatactac ttatcagtgt tagagaactc actccgccgt cttccgcgcg gcccgatccg
      661 gtgcgaccgg atggacaagc ccgtggcaag ccgggtcgcc catcgcgcgc aggccctctt
      721 ccatctagat gcgcaagact catgtattat tccgttcact ttgagaaata ttgtaactat
      781 ttttcctttg actcccgggc tgaggtgtcg ccctgttctc tcagccaggc cagcagcttc
      841 gcccgcaggt cggccactcg atcccgctgg gccaagtcgg caatcaaatc gcgcagctcg
      901 tgcgggtcgc tgccgacgtt gaagagttgc cagcggtcga tcttcggata ccaggcgagc
      961 ttccaatcgc cctcgcggat catccgctgt gaatcctgaa agtagccgac gatgaacggg
     1021 tggacttcgg ccttctcgcc acggagcacg ggcgcgaggc tgcggccgtc catcggcggc
     1081 gtagcgagtc cggccaggtc gcagaacgtc gggaacaaat cgcggagata acactgggcg
     1141 gcgcgcttct cgcccgcggg aatgcccggg ccggcgatca cgaaggggac gttgatcgtg
     1201 tgctcgtaca tgttctgctt gccgaccagg ccatggctgc cgatcgccag gccgtggtcg
     1261 gccgtgaaga cgatgagcgt gctcgcgcgc tggccggttt cgtcgagcgc tttcagaatg
     1321 cggccgagct gggcgtccag gtgtgagatc accgcgtaat agcaggccag ctcctgccgc
     1381 aggtcgccct cggtccgcgg gaagggcagc agcacctcgt cccgaccgcg ggcgttgccg
     1441 tggtcaaacg gatgctcgcc ccgaaagttc gccggcagcg gcagtttcgc cgcgtcgtac
     1501 gttttctcgt agcccggcgg ccagatccgc ggatcgtgcg gggccgtgaa gttgacgtgc
     1561 acgaagaacg gctgctcgct cttctgcgaa atgagccgga tcgccgcgtc ggcaaattcc
     1621 gcgctgatgt tgggcgtcag gccggtgcct ttctcgggaa accgctggcc ggcgtcggtc
     1681 tgaaactgcc agccgcgata gccggtcgcc ggccggccgc ggaaatcgac atacggttcc
     1741 tcgccaaccc ggccgccggc atagagcccg tcgcactcgt cgtagccgac cgtgctaggc
     1801 cgacccgccg tgtgccactt gccgacgtac cacgtgcggt agccggccgc cttcagcagc
     1861 cgcggcaaga gcggcacgcc ctcgttcagc ttaagctcgc tataagtgcc gctgcgaaag
     1921 ccggtgcagc cggtgagcag ttcggcccgg ctgggatagc agagcggatg ggcgcaagtc
     1981 gcccgcgtga agaccgtgcc gcggcggacc agctcgtcca ggtgcggcgt ctggatgtgg
     2041 gagttgccca gcgcgcgaat cgtatccggc cgctggtcgt cggcgatcag cagcaagata
     2101 ttcggcaggc gggcgaaggc ggggccagcg aggcagagag ttgcggcga
//
LOCUS       DASRJB010000200         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_56325, whole genome shotgun sequence.
ACCESSION   DASRJB010000200 DASRJB010000000
VERSION     DASRJB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56325"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFB80_05500"
     CDS             <1..393
                     /locus_tag="VFB80_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33250.1"
                     /translation="ATLEGRPVDAHRLEDGRTLVALQHAQKVVEVDSAGQKVWELAGV
                     GMVFSAQRLESGNTLICAVQTPQVREFDRRGNVVWAQGKFVNPYGAQRLAGGNTVVVD
                     ATGVTEIDPLGATVNRLEMPSLSRMSRY"
     gene            520..3084
                     /locus_tag="VFB80_05505"
     CDS             520..3084
                     /locus_tag="VFB80_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN33251.1"
                     /translation="MNQFPGILSAIAPWQRTLLEWLGYDFSRSPENAQPELLWTNLPA
                     SWGVFVLIAVVAAILYAVFSVYRREMDTCPMWVRMLLATLRAGVVLLLTCIFLGPALV
                     YLTSRSIQPTIVFARDASQSMNTADRYADEAAAKIAAAALAKSESDVRQNRPTRVAIV
                     NEVLAADGGRLLADLAERGKVTALNFADQVTKVDAKIEAASGKRKLPDKSNSSDARSN
                     READASRSPRLPQLVAEGRGSDLFGLIRQSLTADNPAAIVIFTDGQHTGKDDPREAAR
                     EAQARGVPLLIVGVGDPSRPRNLKLANLYVRPQVWQEEPFEIDAIVVAEGIDAGEVRV
                     ELVEQRVGDNDLAIGSGTVVQSLQLPVPPGGGRLPAQFSHTPKEAGRYIYSVKVEPVA
                     DELAEDDNQLASAVVKVLGRERVRVLLVAGAPTWEYRLVQKLLSRDKTITVSCWLQTL
                     DEERAQEGTLPITHLPINKQEMFQYDVILLFDPNPQEFDTAWIELLKQFVGEHSGGLL
                     FMAGPKHSGRLLTNSRTLGLGQVLPVSFGDVGALEVAALLSTNQRAWNMKVVPSNADH
                     PVMRFYPERDETLRRWETLPGIFWSFPSKDAKPTSQVLVEHSDPTLRSVEGSRPLMVA
                     GRYGSGHTLYLGFNGTWRWRKAGRQAEFFDKFWIQAIRYLVEGRSLEGRRRGYVETDR
                     DRYEIGERITITARLQDASFNPLALPKIDAALQVGGDAPETVPLVALPNQPGGFEATL
                     LAKKTGVHTIRVALPASEADSGVIETPFTVELPSVETNQVWLNKPLLMDLASLSGGKY
                     FDVNQLDQLAAAVPDKTESVEVRSPPKPVWDVRGMLVALVGLLGFEWLLRKRYKLL"
     gene            3141..>5105
                     /locus_tag="VFB80_05510"
     CDS             3141..>5105
                     /locus_tag="VFB80_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33252.1"
                     /translation="MSDIPYPSLSQHTSGVSARLARLRSQVAAWFWVDGLSRVLWLAI
                     GLAAADLVLDWLFRMDRAQRAVMLVLMVAAIGWLVQRRLIRPLAATASDDALALEVEA
                     HNKELGQGLISALQLARLENAEKRGMSAALVEQTVLRGTKAAEGVNFASVLDGREFRL
                     NVLLLTLAVLALGGIAVGVRATEPLAIWFNRNILLGDRYWPQKTYLKIERVDKNGAVV
                     FPRGEDWTQLVTVTEDSVVIPESVVLEFRRTKSRGPLAMKKKGERQFEATFANVIEPF
                     EFRARGGDAVTEWVRVELVEQPGVAELRIVVTPPKYTGGSPEELPPGRGPYYVLAGSS
                     LELSGAANKPLVRGDLRYDGELFIQPEPKTAAKAQHRGTAALLRDAGTRLAGQVPADK
                     LVIGTYVFDLEDTLGLTSRRPTTFAIRKRADREPRVRMRLIGVGGMVTVKAVVPFTCR
                     VSDDFGITSGRVVYRWKGEDPMQEATTGGVEFEQLKGKFGGTELSLDDGIEIEPLKVP
                     TGTGLSFRFESADNDDVSGPNVGRSSEFLLRIVTEDELRTDILRREKEQRQEFERLTK
                     NQDELLTDCRALEAAVKTRPDLAAEQKDQLMQYHKRQKLVGQNTTAIAERLASIVIEI
                     QNNRLEEKGGRFQTRLTTEIIEPMRVVSGEL"
BASE COUNT          880 a         1718 c         1727 g          780 t
ORIGIN      
        1 gcgacgctcg aaggccggcc cgtcgatgct caccgcctcg aggacggccg cacgctcgtc
       61 gccctccagc acgcgcagaa ggtcgtcgag gtggacagcg cgggccagaa ggtctgggag
      121 ctggccggcg tgggcatggt cttctccgcc cagcggctgg agagcggcaa cacgctgatc
      181 tgcgccgtgc agacgccgca ggttcgcgag ttcgatcgcc gcggcaacgt cgtctgggcg
      241 cagggcaagt tcgtgaaccc ctacggcgcg cagcgcttgg ccggcggcaa caccgtcgtc
      301 gtcgatgcca ccggcgtcac cgagatcgac ccgctcggcg ccacggtcaa ccgcctggag
      361 atgcccagcc tgagccgcat gagcagatac tagcccctcg gtcccaagct ccgcttggga
      421 ccgcaagtct ggaagctccg cttccagtcc aacacgaaaa ggaagcagag cttccaaacg
      481 cgggttccca agcagagctt gggaaccaga agtgaaaccg tgaatcaatt tcctggaatc
      541 ctatcggcta tcgctccctg gcagcggacg ctgctggagt ggctgggcta cgacttcagc
      601 cgctcgccgg agaacgcgca gccggagctg ctctggacga acctgccggc gagctggggc
      661 gtgttcgtct tgatcgcggt ggtggccgct atcctgtacg ccgtcttctc ggtctaccgc
      721 cgggagatgg acacctgccc gatgtgggtc cggatgctgc tcgcgacgct gcgggcggga
      781 gtcgtgctgc tgctcacgtg catctttctg gggccggccc tcgtttatct cacgagccgc
      841 tcgatccagc cgacaatcgt cttcgcccgc gatgcgtcgc agtcgatgaa cacggccgat
      901 cgctacgccg acgaagcggc ggcgaagatc gccgcggcgg cgctggcgaa gagcgaatcg
      961 gacgtgcggc agaatcgtcc gacgcgggtg gccatcgtca acgaagtgct ggcggcggac
     1021 ggcgggcgcc tgctggccga tctggccgag cgcggcaagg tgaccgccct gaactttgcc
     1081 gaccaggtga cgaaggtcga tgccaagatc gaggcggcga gcgggaagcg taagcttccc
     1141 gataagtcga actcgtccga tgcgcggagt aatcgggagg ctgacgcctc ccgctcgccg
     1201 cgtctgccgc aactcgtcgc cgagggccgc ggctccgacc tgttcggcct gatccgccag
     1261 tcgctcacgg cagacaaccc ggcggcgatc gtcatcttca ccgacggcca gcacaccggc
     1321 aaagacgacc cccgcgaggc cgcccgcgag gcccaggccc gcggcgtgcc gctgctgatc
     1381 gtgggcgtcg gcgatcccag ccgcccccgc aacctgaagc tggccaacct ctacgtccgg
     1441 ccgcaagtct ggcaggagga gccgttcgaa atcgacgcca tcgtcgtcgc cgaagggatc
     1501 gacgcgggcg aggtccgcgt ggagctggtc gagcagcgcg tcggcgacaa cgacctggcg
     1561 atcggcagcg gcacggtcgt gcagtcgctc cagctgccgg tgccgccggg cggcggccgc
     1621 ctgcccgcac agttctcgca cacgccgaaa gaggccggcc gctacatcta ttccgtgaag
     1681 gtcgaaccgg ttgcggacga gctggccgaa gacgacaacc agctggccag cgccgtggtg
     1741 aaggtgctcg gccgcgagcg agtccgcgtg ctcctcgtcg ccggcgcgcc gacctgggaa
     1801 taccgcctgg ttcagaagct gctctcccgc gacaagacga tcaccgtctc ctgctggctc
     1861 cagacgctcg acgaagagcg ggcccaggaa gggacgctgc cgatcacgca tttgccgatc
     1921 aacaagcagg aaatgttcca gtacgacgtg atcctgctct tcgatcccaa tccgcaggaa
     1981 ttcgacacgg cctggatcga attgctcaag cagttcgtcg gcgagcattc cggcggcctg
     2041 ttgttcatgg ccggccccaa gcactctggc cgcctgctga ccaacagccg cacgctcggc
     2101 ctgggacagg tgctccccgt gagcttcggc gacgtggggg ctctggaagt tgcggcgctg
     2161 ctctcgacga accagcgggc ctggaacatg aaggtcgtcc cctccaatgc cgaccatccg
     2221 gtgatgcggt tctatcccga gcgcgacgaa acgctccgcc gctgggaaac gctgccgggc
     2281 attttctgga gcttcccctc gaaagacgcc aagccgactt cgcaggtgct ggtcgagcac
     2341 agcgacccga cgctccgcag cgtcgagggc tcgcgcccgc tgatggtcgc cggccgctac
     2401 ggctcgggcc acacgctcta tctcggcttc aacggcacct ggcggtggcg gaaggccggg
     2461 cggcaggccg agtttttcga caagttctgg attcaagcga tccgctacct cgtcgagggc
     2521 cgctcgctgg aaggccgccg ccgcggttac gtcgagaccg accgcgaccg ctacgagatc
     2581 ggcgagcgga tcaccatcac cgcccgcctg caggacgcgt cgttcaatcc gctggcgctg
     2641 ccgaagatcg acgccgcgct gcaagtcggc ggcgacgcgc cggaaaccgt gccgctggtg
     2701 gcgctgccga atcagcccgg cggcttcgag gccacgctgc tcgccaagaa gaccggcgtc
     2761 cataccattc gcgtcgctct gccggccagc gaggccgaca gcggcgtgat cgagacgccg
     2821 ttcacggtcg agttgcccag cgtcgagacg aaccaggtct ggctgaacaa gccgctgctc
     2881 atggatttgg ccagcctctc gggcggcaag tacttcgacg tgaaccagct cgaccagctc
     2941 gccgccgccg tgcccgacaa gaccgagtcc gtcgaggtcc gcagcccgcc caagccagtg
     3001 tgggacgtcc gcggcatgct cgtcgcgctc gtcgggttgc tcggcttcga atggctgctc
     3061 cgcaagcgat acaaactcct gtagggaacg ccctccgcgg cgttccctaa ccgccacaga
     3121 gggcggtccc tacaaacacc atgtccgaca tcccctaccc ttccctgtcg cagcacacct
     3181 cgggcgtttc cgcccgcctg gcccggctgc ggtcgcaggt cgcggcctgg ttctgggtcg
     3241 atgggctcag ccgcgtgctc tggctggcaa ttggcctggc cgcggccgac ctggtgctcg
     3301 actggctgtt ccgcatggac cgcgcccagc gggccgtgat gctggtcttg atggtcgccg
     3361 ccatcggctg gctcgtgcag cggcggctga tccgcccact ggcggcgacg gcgagcgacg
     3421 acgccctggc cctggaagtc gaagcccaca acaaggagct gggccagggg ctgatttcgg
     3481 ccctccagct cgcacggctg gagaacgccg agaaacgcgg catgtccgcc gcgctcgtcg
     3541 aacagacggt ccttcgcggg accaaagccg ccgagggggt caacttcgcc agcgtgctcg
     3601 acggccgcga attccggcta aatgtcttgc tgcttaccct ggcggtgctc gcgctcggcg
     3661 gcatcgcggt cggcgtccgg gccaccgaac ccttggcgat ctggttcaac cgcaacatcc
     3721 tgctgggcga ccgctactgg ccgcaaaaga cctacttgaa gatcgagcgt gtggacaaaa
     3781 acggcgcggt cgtctttccc cgcggcgagg attggacgca gctggttacc gtcaccgaag
     3841 acagcgtcgt cattcccgaa tcggtcgtgc tcgagtttcg ccgcacgaag agccgcgggc
     3901 ccttggcgat gaagaagaag ggcgagcggc agttcgaggc gacgttcgcc aacgtgatcg
     3961 agccgttcga gttccgcgcc cgcggcggcg atgccgtcac cgagtgggtc cgcgtggagc
     4021 tggtcgaaca gccgggagtc gccgagctga gaattgtggt cacaccgccg aagtacacgg
     4081 gcggctcacc ggaagaattg cctcccgggc gcggccccta ttacgtgctg gccggcagca
     4141 gcctggagct tagcggtgcg gccaataagc cgctggtgcg gggcgacctg cgctacgacg
     4201 gcgagctgtt cattcaaccg gagcccaaga ccgcggccaa ggcccaacat cgcggcacgg
     4261 cggccctgct gcgggacgcg ggcacgcgcc tcgctggcca ggtgccggcc gacaagctgg
     4321 tcatcgggac ctacgtcttc gacctggaag acacgctcgg gctgaccagc cgccggccga
     4381 cgacctttgc cattcgcaag cgggccgacc gcgagccgcg agtgcggatg cggctgatcg
     4441 gcgtgggcgg gatggtgacg gtgaaggccg tggtgccgtt tacctgccgc gtcagcgacg
     4501 attttggcat cacgtcgggc cgcgtcgtct atcgctggaa gggcgaagac ccgatgcagg
     4561 aggcaacgac cggcggggtg gagttcgagc aactgaaggg caaattcggc ggaacggagc
     4621 tctcgctcga cgacgggatc gagatcgagc cgctgaaggt cccgacggga accggcctga
     4681 gctttcgctt cgaatcggcc gacaacgacg acgtgagcgg gccgaacgtc ggccgctcgt
     4741 cggagttcct gctgcgaatc gtcaccgaag acgagctgcg gaccgacatc ctccgccgcg
     4801 agaaggagca gcggcaggag ttcgagcggc tgacaaagaa ccaggacgag ctgctgaccg
     4861 actgccgggc gctggaggcc gcggtgaaga cccggcccga cctggccgcg gagcagaagg
     4921 accagctgat gcagtaccac aagcggcaga agctcgtcgg ccagaacacg acggccatcg
     4981 ccgagcggct ggcctcgatc gtcatcgaga tccagaacaa ccggctggag gaaaaaggcg
     5041 gccggttcca gacgcggctg acgaccgaga tcatcgagcc gatgcgggtc gtctccggcg
     5101 agctg
//
LOCUS       DASRJB010000201         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2312784, whole genome shotgun sequence.
ACCESSION   DASRJB010000201 DASRJB010000000
VERSION     DASRJB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312784"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFB80_05515"
     CDS             <1..498
                     /locus_tag="VFB80_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HZN33253.1"
                     /translation="RRGVQPFSVGKVIFATWRPHEAVVLEAIQSLGIEYQIIFNKRAV
                     MVLPSGVNKATGLKRALKRLQISPHHVVGIGDAENDHAFLESCAVAVAVDNALPVVKE
                     RCDLVMSQADGAGVAELIDRLLADDLQGLGLRRPRRELAAGRAEQPASHDQNATRSGV
                     AKLPG"
     gene            510..1202
                     /locus_tag="VFB80_05520"
     CDS             510..1202
                     /locus_tag="VFB80_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33254.1"
                     /translation="MNVLVIDIGGSSAKLWHTSHQEHRKFDSGKDLTPEAMIAKVKGA
                     AEDWSYEAVALGVPCRVSGGRVVEEPQNLGPGWVGCNLSALFEQPVRIMNDASLQALG
                     SYDGGRMLFLGLGTGVGSALIADHLVLSLDLGRLHWGEQRLWEWLGDKSFEKLGSKKW
                     QKAVHEIVPSLQHVVMADYVVLGGGNAKEVEELPDGVRRGHNRAVVEGGRRLWEDLPD
                     PAKQTPAEWLIL"
     gene            1358..1657
                     /locus_tag="VFB80_05525"
     CDS             1358..1657
                     /locus_tag="VFB80_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33255.1"
                     /translation="MFAKISLMVVVGLCAAAIAATALAADPATHEGMVVSVSAGRLVM
                     KDKAGKEHSHAVGSDAKVTVHGKPARLEDLKAGMPIRVTTEGDKVLAVATIDDMK"
     gene            1740..>2011
                     /locus_tag="VFB80_05530"
     CDS             1740..>2011
                     /locus_tag="VFB80_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZN33256.1"
                     /translation="MNRVFDKTAALERMAFDAQLFREMIDLLREDGPRRLRTLSAGLD
                     AGDWPRVHQAAHSLKGLAANFNATRTVAAAAEVEKLARSGERDGLVP"
BASE COUNT          354 a          608 c          720 g          329 t
ORIGIN      
        1 cgccgcggcg tgcagccgtt ctcggtcggg aaagtcatat tcgcgacctg gcggccgcac
       61 gaggccgtcg tgctcgaggc gatccagtcg ttgggcatcg aatatcagat cattttcaac
      121 aagcgggccg tcatggtgct ccccagcggc gtgaacaagg cgacggggct caagcgggcc
      181 ctcaagcggc tgcaaatctc gccgcaccac gtggtgggaa tcggcgacgc cgagaacgac
      241 catgcgttcc tcgagtcctg cgccgtcgcc gtcgcggtgg acaacgccct gccggtggtg
      301 aaggagcgct gcgacctggt catgtcccag gccgacggag ccggcgtggc ggagctcatc
      361 gaccggttgc tcgccgacga tctgcagggg ctgggcctgc gccgcccgcg gagagagctg
      421 gcagccggcc gcgccgaaca gccggcgagc cacgatcaga acgcgacccg ctctggcgtc
      481 gcaaagttgc ctggttaaag cgaggcgcta tgaacgttct cgtgatcgat atcggcggca
      541 gctccgcgaa gctgtggcac accagccacc aggagcaccg caaattcgat tcgggcaagg
      601 atctgacgcc cgaggcaatg atcgccaagg tcaagggggc ggcggaggat tggtcgtatg
      661 aagcggttgc cctcggcgtg ccgtgccggg tcagcggcgg ccgcgtggtg gaggaaccgc
      721 aaaacctggg gcccggctgg gtcggttgta atctgtccgc cctcttcgag cagccggtgc
      781 ggatcatgaa cgacgccagc ttgcaggcgc tgggcagcta cgacggcggg cggatgctgt
      841 ttttggggct gggcacgggt gtcggctcgg cgctgatcgc cgaccacctg gtgctgtcgc
      901 tcgatttggg gcggctgcac tggggagagc agcggctctg ggagtggctc ggcgacaaga
      961 gcttcgaaaa actgggcagc aaaaagtggc aaaaggccgt gcatgagatc gtgccgtcgc
     1021 tccagcacgt ggtgatggcg gactatgtcg tgctgggggg cggcaacgcc aaggaggtcg
     1081 aggagctgcc cgacggagtg cgccgcggcc acaaccgggc cgtcgtcgag ggaggccgcc
     1141 gactgtggga ggatttgccc gacccggcca aacaaacgcc tgccgagtgg ctcattttgt
     1201 aaggcagttg ccctcatttc ggctggtcaa agttcagcca gcgcggattg ccgggaaaca
     1261 gtcgcctcgc gcgaagccgc ccggcgcccg cagtggcatc gatgttgcat taccaactcc
     1321 tggcgaatcg tcatgttcct gtcaggagaa aatcgccatg tttgcaaaaa tttcgttgat
     1381 ggtcgtggtg ggcctgtgcg cggcggcgat cgcagcgaca gcgctggctg ccgatccggc
     1441 gacgcacgaa gggatggttg tctctgtcag cgcggggcgg ctcgtcatga aggacaaggc
     1501 cgggaaggag cactcgcacg ccgtcggctc ggatgccaag gtcacggtcc acggcaagcc
     1561 cgcgcggctc gaagatttga aagccgggat gccgatccgc gtcacgaccg agggcgacaa
     1621 agtgctggcc gtcgccacca tcgacgatat gaaatgacgc tcgcccgccg ggagagcccg
     1681 cgactatact ggctcgtccg ccgcggacct gttccagcct cgtttcgttg tgcgcgatta
     1741 tgaaccgagt cttcgacaaa acggcggccc tggagcgaat ggcgttcgac gcgcagctgt
     1801 ttcgcgagat gattgacttg ctgcgcgagg atgggccgcg gcggctgcgg acgctctctg
     1861 ccgggctgga cgccggcgac tggccgcgcg tgcaccaggc ggcacattcg ctgaagggcc
     1921 tggcggcaaa cttcaacgcc acgcggaccg tcgccgctgc cgcggaagtg gaaaaactgg
     1981 cccgctcggg cgagcgtgac gggctggtgc c
//
LOCUS       DASRJB010000202        11898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2594792, whole genome shotgun sequence.
ACCESSION   DASRJB010000202 DASRJB010000000
VERSION     DASRJB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594792"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFB80_05535"
     CDS             complement(<1..191)
                     /locus_tag="VFB80_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33257.1"
                     /translation="MLFRFTPHFAAALIALHLLAGRVTAEDWPGFRGPTGMGQSAEAG
                     FPLEWSADGKNILWKSPLP"
     gene            293..1564
                     /locus_tag="VFB80_05540"
     CDS             293..1564
                     /locus_tag="VFB80_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN33258.1"
                     /translation="MAAALPIFLDNHSTTPVDPRVVEAMLPYFTEKFGNAGSINHSFG
                     TDAKDAVDWARDLIAAGIGAQPREIVFTSGATESNNLAIRGLADRTRRTGDHIVSAAT
                     EHKAVLDPLRRLARRGFEITLLDVEPHGNARAGRLDPQRVAAAIRDETLLVSVMLANN
                     EIGVIQPIREIAAICARRGVPLHCDATQAVGKIPVDVEELGIDLLSFSAHKIYGPKGI
                     GGLYVRKSASGRRKPPDHVNPGLPNDREADASRSPVRLDPLIEGGGQEGGLRSGTLNV
                     PGIVGFAKALELCLAEMPSETARLLALRDRLWNGLSTEVPDVMLNGPEFEPGLRLPGN
                     LNCAFPLVSGEALMISLKTIAVSSGSACTSANPEPSHVLRAIGLSEDLTRASLRFGIG
                     RFNTEEEIDYAVAAVAENVHRLRRLSSLAKT"
     gene            1721..2056
                     /locus_tag="VFB80_05545"
     CDS             1721..2056
                     /locus_tag="VFB80_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZN33259.1"
                     /translation="MSSITVTEKAVSALKQHKQSAQATPDTFLRVKLIAGGCSGHEHR
                     LMLANDYDEAKDSRYDFYGEPVVVDKKSELYLEGATLDYIQGLEQSGFHLSVPMAKKT
                     CGCGSSYQF"
     gene            2079..2519
                     /locus_tag="VFB80_05550"
     CDS             2079..2519
                     /locus_tag="VFB80_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33260.1"
                     /translation="MRPIHLQSAAITIAAVAFSWWPAAAHADVLPPKPSTLWGQKEIP
                     STQSIALAGVCLSAAVITVGLLASRLGPLQSAGGRAGLAIFSLMAVAAIGATAFGFYR
                     QAEHDREQWQQYDHAVKNRRWTPPPRWDGPPPESVPPPASSQSP"
     gene            2543..3010
                     /locus_tag="VFB80_05555"
     CDS             2543..3010
                     /locus_tag="VFB80_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33261.1"
                     /translation="MSQTAVSATAPAPPPGAKRGFGLRFSLRGLLLVMLLVAVYFGGR
                     ASMNWRNPFAPRLDGDWEAKLPAGFVQPTTVQSLGDNQYLLSSRASIFNGKYAWQNGK
                     LVVVEPADDRMLGLVWNWDGQQLLLISEPKNTPTGSSYTGTVLTRPAAKPAAP"
     gene            3029..3559
                     /locus_tag="VFB80_05560"
     CDS             3029..3559
                     /locus_tag="VFB80_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN33262.1"
                     /translation="MRVGIFADTHDHLANIRAVVDYFNGAQVELVLFAGDLVSTFAVP
                     PLRKLRAPFVGCFGDNEGNKVGLFAGFTLVGKIGEPPVRHTATDGTRFILCHMERQLR
                     GQADDWDVAVFGHTHKPRIKTDEAGRLLINPGETSGWTFGRPTIVVLETATKLAEVVT
                     IRPVEQAEQAQLRGAG"
     gene            3764..4042
                     /locus_tag="VFB80_05565"
     CDS             3764..4042
                     /locus_tag="VFB80_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HZN33263.1"
                     /translation="MLKKICLVALAAALVLPVLGTAQEKKGKTPEEQFADLDKNGDKK
                     LSKEEYVANREGEKKTKAEERFAAADKDKDGSLTLEEFKAIPGKKKAP"
     gene            complement(4130..4621)
                     /locus_tag="VFB80_05570"
     CDS             complement(4130..4621)
                     /locus_tag="VFB80_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN33264.1"
                     /translation="MKTLLLLRHAKSSWKDSEIDDHDRPLNNRGKRDAPRMGQLLKDE
                     QLFPDYLITSSAKRCRKTAEHVIHASGYRGETRITGEIYEADAEKLRESLAALPAEPA
                     RVLLIGHNPGLEEFVERLTGAYTPLTTAALAQLELPIDRWRDLTAGVRGTLVRVWQPR
                     ELD"
     gene            complement(4624..6750)
                     /gene="ppk1"
                     /locus_tag="VFB80_05575"
     CDS             complement(4624..6750)
                     /gene="ppk1"
                     /locus_tag="VFB80_05575"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZN33265.1"
                     /translation="MTEPASSAPPPPRFFNRELSWLEFNQRVLDEARDESLPLLERLK
                     FLAITASNLDEFFMVRVGGLRLLVEKGITTPDPAGMTPADQLAEISTRAHQMVAQQQA
                     CFAELEQKLAEGGIRRVRGQDLTDRQAKTVEQVFDGEIFSVLTPRAIRSADEFPLLVN
                     QMLHVCVQLAADATSEGQPRFAIIPLGRTMPRFLTLGSDRGHSFVLLEDVVARYLGRF
                     FPGEKVEAHAVFRITRNADFTLRDDLAQDLLAGMEGILTARKHGQCVRLEIAADAGEK
                     VLAFLTQSLGAVAADILPTAAPIDLAAFMSLTDRPGFDALKYEPWPPKAAPEIDPGTR
                     MFDILARRDVLLYHPYQAFDPVLRLIEEAAADPDVLAIKQILYRTSRNSPIVAALARA
                     AQRGKYVTVIVELKARFDEARNIEWARNLEENGVQVIYGVKGLKTHAKTCIIVRREPH
                     GVVRYVHFGTGNYNEITARIYSDASLLTSNEELGADAVAFFNAITGYSQPQRYRKIEA
                     APTGLRPRIVELIEAETHRRQQGQKAFIDSKVNSLVDPEIINALYAASQAGVPVRLNV
                     RGACCLRPGVPGLSENISVMSIVDRYLEHARILHFHHGGDELVFLSSADWMQRNLDRR
                     IELLVPVEDGPCKLRLIEILETYFDDNVKARYLHPDGSYKLAKPGRKKRRRSQEVLYE
                     DAIRRVKESEQSQTTTFEPHRAPGTE"
     gene            complement(6793..8382)
                     /locus_tag="VFB80_05580"
     CDS             complement(6793..8382)
                     /locus_tag="VFB80_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZN33266.1"
                     /translation="MSNRPAETVLTPSAAAAPARQVGVIDIGTSSIRLAVAEINAQRQ
                     VRQLETLAQAVNLGKDSFIRGSISKGTIEECVRVLKSYRRILQEYQIENPAQVRVVAT
                     SAVREAANRLAFVDRIYIATGFQVEPLDEAEVIRITFLGMQPFLLSDPGLASGRAVVT
                     EVGGGSTELLVVKGGDVVYSHTYRLGSLRLRETLEALRAPTLKVRNIMETHIHRTVEE
                     VAEHISRDGAIEMIAMGGDVRFAVRQLQPEWDGRSLARLPVKGLEDFTNRILGQSEDK
                     LVRKYRLSYPEAETLGPALLAYVLLAKELQLTSLLVTNVNLRDGLLKEMAGSETLNKE
                     FSQQIVRSAVDLGRKFQFDEQHAVHVAELTGKLFQQLKEEHQLPPRYEIVLTVAALLH
                     EIGLFVSNRSYHKHSLYLIRHSELFGLGKKDLLLAALVARYHRRASPQAVHEGYSTLD
                     RDERVAVAKMAALLRLADALDDSRSQRIHELQVERDGDRLVISIPMVEDLSLETLALK
                     QNGSLFEETFGLPVLLRMARV"
     gene            complement(8487..9503)
                     /locus_tag="VFB80_05585"
     CDS             complement(8487..9503)
                     /locus_tag="VFB80_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN33267.1"
                     /translation="MALLLRRFNGMSDGAKHWVGFDLGGTKMMAAVFNPEFRLLGRRR
                     RKTKGSEGAKAGLERIVQSIRDALDEGKVDADSLAGIGVGCPGNIDLERGVVIDSANL
                     GWRNFQIREALEKEFNCPAVVLNDVDAGVYGEYRFGAGKNARCLVGVFPGTGIGGGCI
                     YEGQILRGKNVSCFEIGHVQVNPAGLLCGCGRIGCLETEASRLAISAAAAMAAFRGEA
                     PNLLAAAGTDLENIRSGTLADAIKAGDTIIERIVERAARLIGSVVGDVVNLLAPDVIV
                     LGGGLVEAMPDIFVEEVESAARRRAAPPFAKTFKVAAAKLGDDAVVRGAAAWAEARGS
                     ETKK"
     gene            9647..11038
                     /locus_tag="VFB80_05590"
     CDS             9647..11038
                     /locus_tag="VFB80_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN33268.1"
                     /translation="MNRPIRSWLMDSAVSALLMALASQFALGQPQESPAELAARIDKL
                     IQQLDDDQFAAREKAEAELAAIGQPATARLLEATKDASAERRLRADKVLQAIKRAGLG
                     LRHVGTLTHPMLAGAVTLVISPDGQFVYVPAFQVSAVSVFHRDAATGALEHRQSIADP
                     GQLGGIVSLRLSADGKFGVGAAFSSKSIALFSRNAETGELKLEGVRRHEPEGAVKFEW
                     PIDAAFSPDGKFVYAVDDRAATVVVFQVEDGKRLNLVQTFAGEERCFDGARGIVSSPD
                     GKTMYVNSRRPGTLAVLDRDPATGKLAVRQLLRDEQDGIHGLAGTTGAIASRDGQFVY
                     SISGRFEGDNAVGVYQVRADGKLSLLEEFVNDERGLKNFTGPNDLTISPDGQHLYASG
                     TTSCSVACFQRDAGSGRLSLVATLQNEATGLGSELGANGLDVSPDGRFFYLTLENGSA
                     ISIFERTVKPAPR"
     gene            complement(11035..11526)
                     /gene="fae"
                     /locus_tag="VFB80_05595"
     CDS             complement(11035..11526)
                     /gene="fae"
                     /locus_tag="VFB80_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HZN33269.1"
                     /translation="MYIGEGLVGEGNEIAHIDLLIGSKDGPVGQAFANALARQSEGHT
                     NLLAVLTPNLAVKPATVLVTKVTIKGMKQAVQMFGPAQAAVAKAVADSVAAGVIPKNK
                     AEDLVIVCGVFIHPAASDDKKIYQYNYEATKLSIANAMAGKPSADEMIAGKDKAVHPF
                     RGF"
     gene            complement(11631..>11898)
                     /locus_tag="VFB80_05600"
     CDS             complement(11631..>11898)
                     /locus_tag="VFB80_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZN33270.1"
                     /translation="EKRKIAEYDRGFIGAHAISQAVLFNLNPTGKNNAAERLDALMDE
                     GGIQVCGNAQNCVAVCPKKIPLTTSIGRAGRATTVRMFQKWFGA"
BASE COUNT         2106 a         3721 c         3932 g         2139 t
ORIGIN      
        1 tgcggcagag gcgacttcca aaggatgttc ttgccgtcgg ccgaccactc cagcggaaag
       61 cccgcctctg cgctctggcc catgcccgtc ggcccgcgaa atccgggcca gtcctcggcg
      121 gtgacgcggc cagccagaag gtgcagcgca atcagcgcgg cggcgaagtg cggtgtgaaa
      181 cgaaacaaca tttcagccgg ctcgtgggag gtcggggcca cactagcata gtcgctgcgc
      241 gtgcgccaaa tagtctgcaa gccacagcgc ccgcctgggt acaatgccat tgatggccgc
      301 cgcgttaccc atttttctcg acaatcactc cacgactccc gtcgatccgc gggtcgtcga
      361 ggcgatgctt ccgtacttca ccgagaagtt cggcaacgcg ggcagcatca accacagctt
      421 tggaacggac gccaaggacg ccgtcgattg ggcccgcgac ttgatcgccg ccggcatcgg
      481 cgcacagccg cgggagattg tgttcaccag cggtgcgacg gagagcaaca acctggcgat
      541 ccgcggcctg gccgaccgga cgcgccgcac gggggaccat atcgtcagcg cggccacgga
      601 gcacaaggcc gtgctcgatc cgctgcggcg gctggcgcgg cgcggctttg agataacgct
      661 gctggacgtc gagccgcatg gcaacgcacg ggccggccgg ctcgatccgc agcgagtcgc
      721 cgcggcgatc cgggacgaga cgctgctggt ttcggtgatg ctggcgaaca acgagatcgg
      781 cgtgatccag ccgatccgcg agattgccgc gatttgcgcc agacgcggag ttccgctgca
      841 ctgcgacgcg acacaggccg tgggcaagat tcccgttgat gtcgaagagc tgggcatcga
      901 tctgctgagc ttttccgcgc acaagatcta tggtccgaag ggcatcggcg ggctgtacgt
      961 tcgaaagtcg gcgagcggga ggcgtaagcc tcccgatcac gtgaatccgg ggcttccgaa
     1021 tgatcgggag gctgacgcct cccgctcgcc ggtgcgcctc gacccgctga tcgaaggcgg
     1081 cgggcaggaa gggggcctgc gcagcggcac gctcaacgtg cccggaatcg tcgggtttgc
     1141 gaaggcgctg gagctgtgcc tcgctgaaat gccgtccgaa acggcgcggc tgctcgccct
     1201 gcgggaccgg ctctggaatg gactctcaac cgaagtgccg gacgtgatgc tcaatggccc
     1261 ggagtttgag ccgggactgc ggctgcctgg caatctcaac tgcgcatttc cgctcgtgag
     1321 cggcgaggcg ctgatgatca gcctcaaaac cattgcggtg tccagcgggt cggcctgcac
     1381 gagcgccaac ccggagccaa gccacgtgct gcgggcgatc ggactttccg aagacctgac
     1441 gcgggccagc ctgcgatttg ggatcgggcg gttcaatacg gaagaggaaa tcgactatgc
     1501 cgtagcggcc gtcgccgaga atgtccatcg cctgcggcga ctgagcagcc tcgccaagac
     1561 ttgacaaaag tcgtggctgg cgaagaattt aggtgcggtc tgcggcagcc ggcggaattc
     1621 cgctttcagc tgcctgcggg gccgttatac tacttttgac atagccgttt gccgaaacaa
     1681 acaacatttg gcgaatacta aatcccagga gttgccagta atgtcgtcga tcaccgtgac
     1741 cgagaaggcc gtcagcgccc tgaagcagca caagcagagc gcccaggcca cccccgatac
     1801 cttcctgcgc gtgaagctga tcgctggcgg atgcagcggc cacgagcacc gcttgatgtt
     1861 ggccaacgat tacgatgaag cgaaggacag ccgctatgac ttctatggag agcccgtcgt
     1921 cgtggataag aagagcgaac tgtacctgga aggggccacg ctcgactata tccagggtct
     1981 ggagcagagc ggctttcacc tgagcgtgcc gatggccaag aagacctgcg gctgcgggag
     2041 ttcgtatcag ttttagtcag cgtcgcggca gcgcgatcat gagaccgatc cacctgcaat
     2101 cggccgcgat aaccatcgcg gccgtcgctt tttcttggtg gcccgccgcg gcccacgccg
     2161 acgtgctgcc gccgaaaccg agcacgttgt gggggcagaa ggagattccc agcacgcaat
     2221 cgatcgcact cgcgggcgtc tgcctgtcgg ccgctgtgat taccgtgggt ctgctcgcct
     2281 cgcggctggg gccgctacag tccgctggcg gaagggcggg tctggcgatc ttctcgctga
     2341 tggccgtggc ggcaattgga gccaccgcct tcggctttta ccggcaagcc gagcacgacc
     2401 gggagcagtg gcagcagtac gaccacgcgg tgaagaaccg ccgctggacc ccgccgccac
     2461 ggtgggacgg gccgccgccg gaatccgtgc cgccaccggc gtcgtctcaa tcgccgtagg
     2521 ccgggcttgc aggaactgac tcatgagcca aaccgccgtt tcggctactg cgcccgctcc
     2581 gccgcctggg gcaaaacgtg gttttggcct gcgattttcg ctgcgggggc tgctgctcgt
     2641 catgttgctc gtggccgtct attttggcgg gcgggcatcc atgaattggc gcaatccctt
     2701 cgcgccgcgg ctcgatggtg attgggaagc gaagctgcca gccgggttcg ttcagccgac
     2761 gacggtgcaa agcctgggcg acaatcagta tctgctcagc tcgcgggcgt cgatcttcaa
     2821 cggcaagtac gcctggcaaa atggcaagct ggtcgtagtc gagccggccg acgaccggat
     2881 gctcggcctg gtctggaatt gggacggcca gcagctattg ctgatctcgg agccgaagaa
     2941 cacgccaacg gggtcgagct ataccggcac ggtgctgact cgcccggccg cgaagccagc
     3001 ggcgccgtga cctgataaga tgtcgcccat gcgcgtgggg atatttgccg acactcacga
     3061 tcatctcgcg aacattcggg ccgtcgtgga ctattttaac ggggcccagg tggagctggt
     3121 gctgttcgcc ggcgacctcg tttcgacgtt tgcggtgccg cccttgcgaa aactgcgcgc
     3181 gcctttcgtt ggctgctttg gcgacaacga agggaacaag gtcggcctat ttgccggttt
     3241 cacgctggtg ggaaagatcg gcgagccgcc cgtgcggcac accgcaaccg acggcacgcg
     3301 gttcatcctc tgccacatgg agcggcagct ccgcgggcag gccgacgatt gggacgtcgc
     3361 ggttttcggc catacgcata agccgcggat caagaccgac gaggcaggcc gcctgctgat
     3421 caacccgggc gaaaccagcg gctggacgtt cggccggccg acgattgtgg tcctggaaac
     3481 ggcgacaaag ctggcggaag tcgtgacaat ccggccggtc gagcaggcgg aacaggccca
     3541 gctgcgcggc gcgggctgaa tcgggctgca tgccaaactc tttccgggaa agcacttaca
     3601 acgatttcgc gcagacacta gaagttggca acggtttcgc ttatgacatt acaagtgagc
     3661 aagggaaact tcgcgcgcat aaagtggggg tcgtgcgcgt cataatccct gcgatgggca
     3721 ttggacccac agtcatttca tcgtctttcg tgaggagcat tccatgttga agaagatttg
     3781 tttggtggcc ctagcggccg cgctggttct gccggtgctc gggacggcgc aagagaagaa
     3841 gggcaagacg cctgaagagc agttcgccga tctggacaag aacggcgaca agaagctgag
     3901 caaggaagaa tacgtcgcca atcgcgaggg cgagaagaaa accaaggccg aggaacgatt
     3961 cgctgcagcg gacaaggaca aggacggcag cctgacgctg gaagaattca aggcaatccc
     4021 gggcaagaag aaggccccgt aggttgcaac catttcaggt tcgcaagtga tttagcgcag
     4081 gcggcggatg tcgatccgcc gccttttgtt ttgctcgtag cggtaaccac taatctaatt
     4141 ccctcggctg ccagacccgt acgagcgtac cgcggactcc ggcggtcaga tcgcgccagc
     4201 gatcgatagg caattcgagc tgtgccagcg cagccgtcgt gagcggcgtg taagcgccgg
     4261 tcaaacgctc gacgaattcc tccaggccgg gattgtggcc gatgaggagc acgcgcgccg
     4321 gctcggcggg cagggcggcc agcgattcgc gcagcttctc agcgtccgcc tcgtaaatct
     4381 cgcccgtgat ccgcgtctcg ccgcgatagc cgctggcgtg aatcacgtgc tcggccgtct
     4441 tgcggcagcg cttggccgac gaggtaatca ggtaatcggg gaatagctgc tcgtccttca
     4501 gcagctggcc catccgcggt gcgtcgcgct tgccgcgatt gttgagcggc cggtcgtggt
     4561 cgtcgatttc ggagtctttc cagctggact tggcgtggcg aaggaggagc agggttttca
     4621 tggtcactcg gttcccggcg cgcggtgcgg ctcgaaagtg gtggtctgag actgctcgga
     4681 ctctttcacg cgacggatgg cgtcttcata caagacttcc tggctgcggc ggcgtttctt
     4741 gcgtccgggc ttggccagtt tgtacgagcc gtcggggtga agatagcggg ccttgacgtt
     4801 gtcgtcgaaa tacgtctcca gaatctcgat cagccgcagt ttgcaggggc cgtcctcgac
     4861 gggcacgagc agctcgatgc ggcggtcgag attccgctgc atccagtcgg cgctggagag
     4921 gaacaccagc tcgtcaccgc cgtgatggaa gtgcaggatg cgggcgtgct ccaggtaccg
     4981 atcgacaatg ctcatgacgc tgatgttctc gctcaagcct ggcacgcccg gccgcaagca
     5041 gcaggccccg cgcacgttca gccgcacggg cactccggcc tgcgaggcgg catacagggc
     5101 gttgatgatc tccggatcga cgagcgaatt gactttggag tcgatgaatg ccttttgccc
     5161 ctgctggcgg cggtgcgttt cggcctcgat cagctccacg atccgcggcc gcaggcccgt
     5221 cggggcggcc tcgatcttgc ggtagcgctg cggctgcgag tagcccgtga tcgcgttgaa
     5281 gaaggcgacg gcgtcggcgc cgagctcctc gttgctggtc agcaggctgg catcgctgta
     5341 gatgcgggcg gtgatctcgt tgtaattgcc cgtgccgaaa tgcacgtagc ggacgacgcc
     5401 gtggggctcg cggcggacga tgatgcaggt cttggcgtgc gtcttgagcc ccttcacgcc
     5461 gtaaatcacc tgcacgccgt tttcttccag gttgcgggcc cattcgatgt tgcgggcttc
     5521 gtcgaagcgg gccttcagct cgacgatcac ggtcacgtac ttgccccgct gtgccgctcg
     5581 cgccagtgcg gcgacgatcg ggctgttgcg gctggtgcga tagagaatct gcttgatcgc
     5641 cagcacgtcc ggatcggccg ccgcctcctc gatcagccgc agcaccggat cgaacgcctg
     5701 gtaggggtgg tagagcagca cgtcgcggcg ggccaggatg tcgaacatcc gcgtgccggg
     5761 gtcgatctcc ggcgcagcct tcggtggcca gggctcgtac ttgagcgcat cgaaaccggg
     5821 gcgatccgtg agcgacatga acgccgccag gtcgatgggc gcggccgtcg ggaggatgtc
     5881 ggctgccacc gcgcccagcg actgcgtcag aaaggcgagc actttttcac cggcgtcggc
     5941 ggcgatctcc aaccgcacgc actggccgtg cttgcgggcc gtcaggatgc cctccatgcc
     6001 ggccaacagg tcctgcgcca ggtcgtcgcg aagcgtgaag tcggcgttgc gcgtgatccg
     6061 gaagacggcg tgagcctcga ccttttcgcc gggaaagaac cggcccaagt atcgcgccac
     6121 gacgtcttcc agcagcacaa acgagtggcc gcggtccgag cccagcgtca ggaaccgcgg
     6181 catcgtccgg cccagcggga tgatggcaaa ccgcggctgc ccctcgctcg tggcatcggc
     6241 cgccagctgc acgcagacat gcagcatctg gttgaccagc agcggaaact cgtccgctga
     6301 gcgaatggcc cgcggcgtga gcaccgagaa aatctcgccg tcaaaaacct gctcgaccgt
     6361 cttggcctgc cggtccgtta aatcctgccc gcggacgcgg cggatgcccc cctcggccag
     6421 cttctgctcc agctcggcaa aacaggcctg ctgctgggcg accatctggt gggcccgcgt
     6481 gctgatctcc gcgagctgat cggcgggcgt catgcccgcc ggatcgggcg tcgtgatccc
     6541 cttctcgacg agcagccgca gtccgcccac gcggaccatg aagaattcgt ccaggttcga
     6601 cgccgtgatc gccaggaact tcagccgctc caggagcggc aggctttcgt cgcgggcctc
     6661 atcgagcacg cgctggttaa actccagcca cgagagttcg cggttgaaga acctgggtgg
     6721 cggaggagca gaggaggcgg gttccgtcat ggtgtccgtt ttctactgga ctcgattggt
     6781 ggtccagccc ggtcaaaccc tcgccattct tagcagcacg gggagcccga atgtctcctc
     6841 gaacagtgag ccgttttgct tgagggccag cgtctccagc gacaggtctt ccaccatcgg
     6901 aatggagatc accagccggt cgccgtcgcg ttcgacttgc agttcgtgga ttcgctggct
     6961 gcgcgaatcg tccagggcgt cggccaatcg caataacgcg gccattttgg cgacggccac
     7021 gcgttcgtcg cggtcgagcg tggaataacc ctcgtgcacc gcctgcggcg aagcccggcg
     7081 atggtagcgg gcgacgagcg cggccagcag caggtccttc ttgcccaggc cgaacagctc
     7141 gctgtggcga atcaggtaga gcgagtgctt gtggtagctg cggttgctca caaacaggcc
     7201 gatctcgtgc agcagcgccg cgacggtcag cacgatctcg taacgcggcg gcagctgatg
     7261 ttcctccttg agctgctgaa agagctttcc ggtaagctcg gccacgtgaa ccgcgtgctg
     7321 ctcgtcgaac tggaatttgc ggcccagatc gactgccgag cgcacgatct gctggctgaa
     7381 ctccttgttg agcgtctcgc tgccggccat ttccttcagc aggccgtcgc ggagattgac
     7441 gttcgtcacc agcaaactgg ttagttgcaa ctccttggct agcaggacgt acgccaggag
     7501 tgccggccca agcgtttccg cctcgggata gctcaagcgg tacttgcgaa ccagcttgtc
     7561 ctcggactgg cccagaatgc ggttcgtgaa gtcctcgagc cccttgaccg gcagccgggc
     7621 caggctgcgg ccgtcccact cgggctgtag ctggcggacg gcgaaccgca cgtcgcctcc
     7681 catcgcgatc atctcgatcg ccccgtcgcg gctgatgtgc tcggcgacct cctctaccgt
     7741 gcggtggatg tgggtctcca tgatgttgcg gaccttgagc gtcggtgccc gcaaggcttc
     7801 gagcgtctcc cgcagccgca agctgccgag ccgataggtg tgcgaataaa cgacgtcgcc
     7861 tcctttgacg accagcagct cggtgctgcc gccgccgact tccgtcacga ccgctctccc
     7921 gctggccagg cccggatcgg aaagcaggaa cggctgcatg cccaggaaag taatgcggat
     7981 aacttccgcc tcgtcgagcg gctccacctg gaagccggtg gcgatgtaga tgcgatcgac
     8041 gaaggccagc cggttggcgg cctcacggac ggcgctggtc gcgacgaccc gcacctgggc
     8101 cgggttctcg atctggtatt cctgcaaaat gcggcggtag cttttgagga cgcggacgca
     8161 ttcctcgatc gtacctttgc tgatcgagcc gcggatgaag gagtcctttc ccaggttcac
     8221 cgcctgagcc agcgtttcga gttgccgcac ctgccgctgg gcgttgatct cggccacggc
     8281 caagcggatc gacgacgttc cgatgtcgat cacgcccacc tgccgcgccg gcgccgcggc
     8341 ggcggacggc gtgagaacgg tctcggcggg gcggttggac atggggcaga tttatgattt
     8401 cagatttcag gtttcagatt tgcaattagc gaagtcaaat ctgaaatctg aaatcccaaa
     8461 tctgaaattg gcatcgcaag cgacattcac tttttggttt cacttccccg ggcctccgcc
     8521 cacgccgcgg cgccgcggac gacggcgtca tctcccagtt tagccgccgc gaccttgaac
     8581 gtcttggcga atggcggggc cgcccgccgg cgggccgcgg attcgacctc ctcgacaaaa
     8641 atgtcgggca tggcctcaac cagcccgccg cccaacacga tcacgtcggg agccagcagg
     8701 ttcactacgt cgccgaccac gctgccgatc aggcgggcag cccgttcgac gatccgctca
     8761 atgatcgtgt cgcccgcctt gatggcgtcg gccagagtgc cgctgcggat gttttccagg
     8821 tcggtcccgg ccgccgcaag caggttcggg gcttcaccgc gaaaggccgc catcgccgcg
     8881 gccgccgaga tcgccaggcg gctggcctcg gtctccaggc agccgatccg cccgcagccg
     8941 cagagcaaac cggcgggatt gacctggacg tggccgattt cgaagcacga gacgttcttt
     9001 ccgcgaagaa tctgcccctc gtagatgcaa ccgccgccga ttcccgtgcc gggaaagacg
     9061 ccgacgagac agcgagcgtt ctttccagcg ccgaagcggt attcgccgta aacgccggca
     9121 tcgacgtcgt tcagcacgac agccgggcag ttgaactcct tttccagcgc ctcgcgaatc
     9181 tggaagttgc gccaaccgag gttggccgag tcgatgacga cgcctctctc gagatcgata
     9241 ttgccggggc agccgacgcc gattccggcg agggagtcgg catcgacctt gccttcgtcc
     9301 agggcatcgc ggatcgactg aacgatccgc tccaggccgg ctttagcgcc ttccgaaccc
     9361 ttcgtcttgc ggcgtcgccg tcccagcagg cggaactccg ggttgaagac cgcggccatc
     9421 atcttggtgc cgccgaggtc gaatcccacc cagtgtttcg cgccgtcgga catgccgttg
     9481 aatcgccgga gaagcagggc caagagaagc ggtaatgata gcaggcgaga gtaattgaga
     9541 ggcaggggat agcacgccgc tatggcgtgc attgcaggca atgattgcca ggcgcggccc
     9601 ggatgcggat aatgccccgc agatcgcaaa aagctggagc tcaagcatga atcgacccat
     9661 tcgcagttgg ctcatggaca gcgctgtctc cgcgctgctg atggcgttgg cgagccagtt
     9721 cgcactgggg caaccgcagg agtcgccagc tgagttggca gcccgcatcg acaagctgat
     9781 ccagcagctc gacgacgacc agttcgctgc ccgcgagaag gcggaggccg agctggccgc
     9841 tatcggccag ccggcgaccg ccaggctgct ggaagcaacg aaggacgcgt cggccgagcg
     9901 gcgactgcgg gccgataagg ttctgcaagc gatcaagcgg gctgggctgg gactccgcca
     9961 cgtgggcacg ctcacccatc ccatgctggc cggcgcggtg accctggtca tcagcccgga
    10021 cgggcaattc gtctatgtgc cggcgttcca ggtcagcgcc gtcagcgtct ttcaccgcga
    10081 tgccgcgacc ggggcgctgg aacaccggca gtcgatcgcc gatcccgggc agctgggcgg
    10141 catcgtgagc ttgcggctaa gcgccgacgg caagttcggc gtgggggcgg ctttcagctc
    10201 gaagtcgatc gcgctctttt cccgcaacgc cgagacgggc gagctcaagc tggagggcgt
    10261 ccgccggcac gagcccgaag gggcggtcaa gttcgagtgg ccgatcgacg cagcgttctc
    10321 gcccgacggc aagttcgtct atgccgtgga cgaccgcgct gcgaccgtgg tcgtgtttca
    10381 ggtcgaggac ggcaagcggc tgaacctggt gcagacattc gcgggcgaag agcgttgctt
    10441 cgacggcgca cgggggatcg tcagcagccc cgacggaaag acgatgtatg tgaacagccg
    10501 ccggccgggc acgctggcgg tgctcgaccg cgatccggct acaggcaagc tcgccgtgcg
    10561 gcaactgctg cgcgacgaac aggatggaat ccacggcctg gcggggacga ccggcgcgat
    10621 cgccagccgg gacggccaat tcgtgtattc gatttccggg cggttcgagg gggacaatgc
    10681 ggtcggcgtc taccaggtga gagccgacgg caagctgtcg ctcttggagg aatttgtcaa
    10741 cgacgagcgg gggctgaaga attttaccgg ccccaacgac ctgactattt caccggacgg
    10801 ccagcacctg tacgcctcgg gcacgacctc gtgctcggtg gcctgctttc aacgcgacgc
    10861 cggcagcggc cggctaagcc tggtggctac gctgcagaac gaggccacgg gcctgggcag
    10921 cgaattgggg gccaacggcc tggacgtcag ccccgacggc cgtttctttt acctgacgct
    10981 ggagaacggc agcgccattt ccatcttcga gcggacggtg aagcccgccc cgcgctagaa
    11041 cccgcggaac ggatggaccg ctttgtcctt gccggcgatc atctcgtcgg ccgagggctt
    11101 gccggccatc gcgttggcga tcgagagctt cgtggcttcg tagttgtact gatagatctt
    11161 cttgtcgtcg ctggcggcgg ggtgaatgaa gacgccgcag acgatgacca ggtcctcggc
    11221 cttgttcttc ggaatgacgc cggccgcgac cgagtcggcg acggccttgg cgacggccgc
    11281 ctgggccgga ccgaacatct gcacggcctg cttcatcccc ttgatcgtga ccttggtgac
    11341 gagcacggtc gccggcttca ccgccaggtt aggcgtcagc accgccagca ggttcgtgtg
    11401 cccttcgctc tgccgggcca gggcgttggc gaacgcctgt cccaccgggc cgtccttgct
    11461 gccgatcagc aggtcgatat gcgcgatttc gttgccttcg ccgaccaggc cttcgccgat
    11521 gtacatggat cgtctccagt gcttgtttga gaggatttcg gccgtgcttg ctcgtcagca
    11581 gggtaacaaa gcaggcgtgg aatgcaacca cccgcaaaat gccgaggccg ctaggctcca
    11641 aaccacttct ggaacattcg cacggtcgtc gcgcggccgg cgcgaccgat cgaggtggtc
    11701 agcgggatct tcttggggca gaccgcgacg caattctgag cattgccgca gacctgaatg
    11761 ccgccttcgt ccatcagggc gtccaaccgc tcggcggcgt tgttcttgcc cgtgggattc
    11821 aagttgaaga gcacggcctg gctgatggca tgcgcgccga taaagccgcg gtcgtattcc
    11881 gcgatctttc gcttctcg
//
LOCUS       DASRJB010000203         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1748884, whole genome shotgun sequence.
ACCESSION   DASRJB010000203 DASRJB010000000
VERSION     DASRJB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748884"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..518)
                     /locus_tag="VFB80_05605"
     CDS             complement(141..518)
                     /locus_tag="VFB80_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZN33271.1"
                     /translation="MAEYDPRYLAGIEHFNKCDFFEAHEVWEELWADYSGPSRKFYQG
                     LIQVAVCLHHFGNGNIRGAKKLYYGCRGYLEPYLPKHEGLDLQKLLAQLETCCREILD
                     SKEEFPKIDIVPDLIPEVHLEDS"
     gene            complement(521..739)
                     /locus_tag="VFB80_05610"
     CDS             complement(521..739)
                     /locus_tag="VFB80_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33272.1"
                     /translation="MPRSLLEAIKQGDWNFEPTVELLASSEGGLQRATTAMPGTAEKL
                     GVLAERLQRGLPLWHPSDRRTYNDTDQD"
     gene            complement(891..1613)
                     /locus_tag="VFB80_05615"
     CDS             complement(891..1613)
                     /locus_tag="VFB80_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HZN33273.1"
                     /translation="MSQRGGPGLLVSVRSAAEAQRALAGGADLIDIKEPGNGALGAAE
                     LGVWGEVLRTVAGRAPVSAAMGELLDNEARDRAAQAFGLRFAKFGLAGCQRREGWLNR
                     WFAAQDALPCGVAGVPVAYADWPAAAAPSPSVALALAAQTQAKTLLIDTFDKRRGTLL
                     DALSWESLREIAEGARRAGVQLVLAGSLDGAAIERLWPLAPAWFGVRGAACLGGRGGT
                     IETALVKSLARIVMSAGRKAAG"
     gene            complement(1610..2152)
                     /locus_tag="VFB80_05620"
     CDS             complement(1610..2152)
                     /locus_tag="VFB80_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33274.1"
                     /translation="MSEVLLRTNHRALAMGMILPGLILVGSVTGLIWALTAGRHWAFS
                     LAFASGTMLALWMLGELVYAAWQPRLAVAAGDLLVFLEPTRPTKVPLEIVECFFLGQG
                     PSELPRLKGREPQTQNVIVRLAESAVEWKHRDVKPAFGHWCEGYITLRGSWCEPITPA
                     IMARLNRRLAELHHERKAAS"
     gene            complement(2223..3191)
                     /locus_tag="VFB80_05625"
     CDS             complement(2223..3191)
                     /locus_tag="VFB80_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33275.1"
                     /translation="MPRPLILAALLSAVFQFPLAAAEPTPRRPKNDEELRFWLDNMLV
                     HHRFSAEEAAAATGLSAAEVTAAAERWKIDPAKAPARAEGAPLLVLPYPGGRHPRIGF
                     LDGAVDPQRETKVSVFAPWKDGGYAVADVPEAIWMEIPGGRELLYLAHTHVATHWTKQ
                     GVTLEPLEWRAIEDCWQMERTLPNKVAFGARVTPQREGVKMEMWLTNGRATPLKGLVV
                     QNCVMLKGLAGFAQQTNDNKLFRAPLVACSSADKTRWIITAWTPNHRTWGNAPCPCLH
                     SDPKFPDCEPGKTVRLRGWLSFYEGADIEGELTRLGKLDWLQQPSK"
     gene            3437..3931
                     /gene="greA"
                     /locus_tag="VFB80_05630"
     CDS             3437..3931
                     /gene="greA"
                     /locus_tag="VFB80_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZN33276.1"
                     /translation="MDEYVPMTRPGFTKLKAEAERLETIEMPLIAEKIAAARSEGDLK
                     ENAEYHAQREAQGLLQAKINLIKSKLARAQIIDPATLPRDQVCFGATVIVKDVDLGDE
                     EEYTLVGAGDENYDQGRILVTSPLGQGLLGKKIGQTAEIPAPKGSYELEVVAIRFDYL
                     EGPA"
     gene            3940..>4351
                     /locus_tag="VFB80_05635"
     CDS             3940..>4351
                     /locus_tag="VFB80_05635"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HZN33277.1"
                     /translation="MAAPLLLTLLSLGQVDLPAARPVPRMQVIPLPAGQARIELGGVE
                     LSRYYFGQDLFRPFLYPLIGPSGKSLTRMGHPRDPNGHSHHNSVWISHHDVGGVAFWN
                     DAKTSKGRILHQKVNRYEDADDEALIEVQNAWIDS"
BASE COUNT          753 a         1454 c         1417 g          727 t
ORIGIN      
        1 ggtggccgtt ccgctcatcg ccggctacgc ctatcaccgc cgcggctggg agcagcgtcc
       61 cgccaagcgg ttcgccgacc tctatgccga agagccgcag ccggtgggga cgtaacggcc
      121 gccgacgctg gcagcgtcgg ctacgagtcc tccaggtgaa cctccggaat caagtccggc
      181 acgatgtcga tcttggggaa ttcctccttg ctgtcgagaa tctctcggca gcaagtttcg
      241 agctgcgcga gcagcttctg caggtcgagg ccctcgtgct tcggcaggta gggttccagg
      301 tagccgcggc agccgtagta gagcttcttc gccccgcgga tgttgccgtt gccgaaatgg
      361 tgcaggcaga cggcgacctg gatcaatccc tgatagaact tccgcgaggg gccgctgtag
      421 tcggcccaca attcttccca cacttcgtgc gcctcgaaga agtcgcactt gttgaagtgc
      481 tcgatgccgg cgaggtaccg cgggtcgtac tcggccacga tcaatcctgg tcggtgtcgt
      541 tgtacgtgcg gcggtcggaa ggatgccaca gcggcagccc ccgctgcaga cgttcggcca
      601 gcacgcccag cttctcagcc gtgccaggca tcgcggtggt cgcccgctgc aggccgccct
      661 cggagctggc gagcaactcg acggtcggct cgaaattcca atcaccctgc ttgattgctt
      721 cgagaaggga gcgcggcaca gctgatctcc tgacgataaa gatgaagcaa ggagcgactg
      781 cggaatgcaa gggacgagtt ggcgggcgct ccgtcgaacc tctgcttccc caaactccgt
      841 ctcggccgcc gggacggcag tattgaggcc ggcccaggca cagtcaagca tcagccggcg
      901 gctttgcgcc ccgcggacat tacgattcgc gccagggact tgacaagcgc cgtttcaatc
      961 gtgccgccgc ggccacccag gcaagccgcg ccgcggacgc cgaaccaggc cggcgcaagc
     1021 ggccacaggc gctcgatcgc cgcgccatcg agcgagccgg ccagcaccag ctgcacgccg
     1081 gctcgccgcg cgccctccgc gatttcccgg agcgactccc aggaaagggc gtcgagcagc
     1141 gttccgcggc gcttgtcgaa cgtgtcgatc agcagcgtct tcgcctgcgt ctgtgcggcc
     1201 agcgcgagcg cgaccgaagg cgacggcgcc gcggccgccg gccaatcggc ataggccacg
     1261 ggcactcccg ccacgccgca aggcagggcg tcctgcgcag cgaaccagcg attgagccag
     1321 ccttcgcgcc gctggcaccc ggcgaggccg aacttggcga atctcaggcc gaatgcctgt
     1381 gccgcgcggt cccgagcttc attgtcgagc aattcgccca tggcagcgct caccggcgcg
     1441 cggccggcga ccgtccgcag gacttcgccc caaacgccca gttcggccgc acccagcgct
     1501 ccattccccg gctccttgat gtcgatcagg tccgcgccgc cggccagcgc ccgctgggcc
     1561 tccgccgcgc tgcgcacgct gacgagcagt cccggtccgc cgcgttggct catgacgccg
     1621 cctttcgctc gtggtgcagc tcggccagcc gccggttgag ccgcgccatg atggcggggg
     1681 tgatcggctc gcaccagctg ccgcggagcg tgatgtagcc ctcgcaccag tggccgaagg
     1741 ctggcttcac gtcgcggtgc ttccattcga cggccgattc cgccaggcgg acgatcacat
     1801 tctgcgtctg gggttcgcgg cccttcagcc ggggcagctc gctcggcccc tgtcccagga
     1861 agaagcattc cacgatctcc agcggcacct tcgtcggccg cgtcggctcc aggaatacga
     1921 gcaagtcgcc cgcggccacg gccaatcgcg gctgccacgc ggcatagacc agctcgccca
     1981 gcatccacag cgccagcatc gtcccgctgg caaacgccag gctgaaggcc cagtgccgtc
     2041 cggccgtcag cgcccaaatc agacccgtca cggacccgac caggatcagt cccggcagga
     2101 tcatccccat cgccagggcg cggtggttcg tgcggagcaa gacttcggac atggatcggt
     2161 tcgagtgagt gacagccggc ggttgtacca caggaggccg ccgcggcaca tcgcggaatg
     2221 ccttacttgg acggctgctg gagccaatcg agcttgccca gccgcgtcag ttcgccctcg
     2281 atatcggccc cctcatagaa cgacagccag ccgcgcaagc ggacggtctt gcccggctcg
     2341 cagtcgggaa acttgggatc gctgtggagg caggggcagg gagcgttgcc ccaggtgcgg
     2401 tggttgggcg tccaggcggt gatgatccag cgggtcttgt cggcgctaga gcaggcgacc
     2461 aggggcgccc ggaagagctt gttgtcgttg gtctgctggg caaagccggc cagccccttg
     2521 agcatcacgc agttctgcac gaccaggccc ttgagcggag tggccctgcc gttggtgagc
     2581 cacatttcca ttttcacgcc ctcccgctgc ggtgtgacgc gggcgccgaa tgcgaccttg
     2641 ttgggcaggg tgcgctccat ttgccagcag tcctcaatcg cccgccactc cagcggctcg
     2701 agcgtcacac cttgcttcgt ccagtgcgtg gccacgtgcg tgtgggccag atagagcagc
     2761 tcgcggcccc cggggatctc catccagatc gcctcgggca cgtcggcgac ggcgtagccg
     2821 ccatctttcc agggcgcgaa gacgctgacc ttcgtttccc gctgcggatc gaccgcgccg
     2881 tcgagaaagc cgatccgcgg gtgccggcca ccgggatagg gcaataccaa gagcggcgcg
     2941 ccctcagctc gcgcgggcgc tttcgccggg tcgatcttcc atcgctcggc tgcggccgtc
     3001 acttccgctg cggagagccc ggtcgctgcc gcggcctcct cggccgagaa gcggtggtgg
     3061 accagcatgt tgtcgagcca gaaccgcagt tcctcgtcgt tcttcggccg ccgcggcgtg
     3121 ggctcggcag ccgccagagg gaactggaaa acggcggaga gcagtgcggc gaggataagc
     3181 gggcggggca tggtttggtt cgggagagaa ttgcaaagtg tcaatcgcaa agtgcaaagt
     3241 gcctgaccca atttgcgttt tgcattttcc acttttcaat ttgcaattcc agccgcccgg
     3301 catctcgcct ttacaccgct gccggcctgg gccaaaataa ctgtctggtt ggacagttcg
     3361 actgacatac gcggcctcct ccctaggagg tcgcgttttt tgtcaggtca ggtcgatttc
     3421 acgcaggtaa gttgccatgg acgaatacgt gccaatgacc cgacccgggt ttacgaagct
     3481 caaggccgag gcggagcggc tggagacgat cgagatgccg ctgattgcgg agaagatcgc
     3541 cgccgcacgt tccgaggggg atctcaagga aaacgccgaa taccacgccc agcgcgaggc
     3601 ccagggcctg ttgcaagcca agatcaatct gatcaagtcc aaactcgccc gggcccagat
     3661 catcgacccg gcaacgctgc cccgcgacca ggtctgcttc ggggcgacgg tcattgtgaa
     3721 agacgtcgat ctgggggacg aagaagagta cacgctggtc ggcgcggggg acgagaacta
     3781 cgaccagggc cggattctgg tcaccagtcc gctgggccaa gggctcctcg gcaagaaaat
     3841 cggccagacc gccgaaattc cggcccccaa gggttcgtac gagctggagg tcgttgcgat
     3901 ccggtttgac tatctggaag gcccggccta agggaagcca tggctgcccc actgctactg
     3961 acgctcctct cgctcgggca ggtggacctg ccagcggcca ggcccgtgcc gcggatgcag
     4021 gtgattccgc tgcccgcggg ccaggcgcgg atcgagttgg gcggtgtgga actttcgcgg
     4081 tattatttcg gccaggactt gtttcggccg tttctctatc cgctgatcgg ccccagcgga
     4141 aaatcgctca cgcgaatggg gcatccgcgc gatccgaacg gacacagcca ccacaattcg
     4201 gtctggatct cgcaccacga cgtcggcggc gtagcgttct ggaacgacgc caaaacgagc
     4261 aaggggcgaa tcttgcacca gaaagtgaac cgctacgaag acgccgacga cgaagccctg
     4321 atcgaggtgc agaacgcctg gatcgactca g
//
LOCUS       DASRJB010000204         7366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_621662, whole genome shotgun sequence.
ACCESSION   DASRJB010000204 DASRJB010000000
VERSION     DASRJB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621662"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..2025)
                     /gene="pckA"
                     /locus_tag="VFB80_05640"
     CDS             complement(427..2025)
                     /gene="pckA"
                     /locus_tag="VFB80_05640"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZN33278.1"
                     /translation="MSEHRIELLSHGIFASGVVRNPAPAILYEDAIRGEDGSLASSGA
                     IIARSGAKTGRSPRDKRITRSPANERDVWWGSVNIPLSEHSFRICRKRAQDYLTRCPK
                     LYVVDGYAGWDPEYRLKIRVVCSRPYHALFMRNMLIRAKQSELGDFGQPDYTIYNAGS
                     FPADAEIEGLSSDTCVALNFESRELIILGTQYAGEMKKGVFTVMHYLMPKRGVLSMHC
                     SANEGPAGEVSLFFGLSGTGKTTLSADPRRRLIGDDEHCWSDRGVFNIEGGCYAKCIN
                     LSADKEPEIFRAIRAGTVLENVVVDPATRMVDYGDSHLTENTRACYPIDFIPNAKVPC
                     VGGHPQHLIFLTCDAFGVLPPVARLTPAQAMYHFISGYTAKVAGTEVGVKDPEATFSA
                     CYGAAFLVFHPMKYALMLADKMQQRQASAWLVNTGWTGGSYGAGSRIKLVHTRAIIDA
                     IHSGQLTRAPTTTEPVFGLAVPTACPGVPAEMLVPRDTWNKPGAYDTKALHLAKLFQV
                     NFGQYADQATPEVLDAGPKPNGSS"
     gene            complement(2110..3177)
                     /locus_tag="VFB80_05645"
     CDS             complement(2110..3177)
                     /locus_tag="VFB80_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN33279.1"
                     /translation="MPDSTPAQLDSPPPEAAVGQEPASDIGQAPASDVVQADLAGNPP
                     PRRWRLGRRRVLLPALLFLLTCASTFFAGATGWMPNQFILFNGPADLYQADFLLSARL
                     AVLQNWPDGLLYMGCIVGILLTHEMGHFVMTVVHRVRASLPFFVPFPISPLGTLGAVI
                     GMDGSRANRRQMFDIGLAGPLAGLVVAIPIIWIGVARLDLKHAPPAEWALELPLAMRL
                     LVDRFHPGEYNPAVGVPFSALNPYLMAGWVGLLVTALNMMPVSQLDGGHVTYTLFGRA
                     AHWVARLFMLAVFIYLGVTTIYYHQMPFWLLMALLVLVMGTDHPPTADDEMPLGTFRT
                     VLGWASLLIPVFCFTPNPFRM"
     gene            complement(3253..4611)
                     /locus_tag="VFB80_05650"
     CDS             complement(3253..4611)
                     /locus_tag="VFB80_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZN33280.1"
                     /translation="MASLFAKAEAEHLRKAQPLAARMRPRTLAEFAGQQHFLGDGKLL
                     RRLLAADRLTSVIFYGPPGTGKTTLARLLATESKKRFVQLSAVTSGVKELRQVLEEAR
                     DGLAGGGQRTLLFIDEIHRFNKAQQDALLPDVEEGIVTLVGATTSNPFFAVISALVSR
                     SQVFEFQPLSPDEIKLVIQRALADKERGLGNYDVRMHDDALQFLAQASDGDARRALSA
                     LEIGVLSSHLAPRDADLTRTVGTTIEFTRELAAESVQRKAGAYDGTGDEHYDIISALI
                     KSVRGSDPDAGLYWLARMLEAGEDVRFLCRRLIILASEDVGNADPAALPLAVAAMQAC
                     EFVGLPECQLPLAQAVTYLACAPKSNASTVAIEAARQDVREGRIVPVPVHLKDRSYYG
                     AKRLGHGEGYQYAHDAEGGIAEQDYLGVQREYYQPVPRGFEAELAERLAAIRKRLRGG
                     EG"
     gene            4576..5160
                     /locus_tag="VFB80_05655"
     CDS             4576..5160
                     /locus_tag="VFB80_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZN33281.1"
                     /translation="MLGFGFGKQAGHELQGSLKRRYYEVGLVSLSYACGRSPAMARKR
                     MITVLYVAGGLAIATVAAILIFLVAQVEDWSRDLTTNSAETTSDAADERLRPIETALP
                     PTELARHVESAVTSLKNWRQVERKGSGGNIEFHLLRTTPVLGFQDDIRVRIEPAGGGA
                     RLTAQSRSRVGKGDLGQNPRNLRELLDAVRSRLR"
     gene            5177..5725
                     /locus_tag="VFB80_05660"
     CDS             5177..5725
                     /locus_tag="VFB80_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33282.1"
                     /translation="MEFLLSIFGVLSCCFSPALALGYVWVVTTWTLGPLIAAARQAPA
                     PTRFLLSDLLWLMIQLQLAMALVAGAVPRDFPTETRVWAAILLGLPTAAFWYACLHVV
                     AQAGIGGTLRRGAVFVLLVPGTVVAIVGVPILAMAVLSLTNARNSQPYELEWTTVALT
                     AGAAATFGMRWLARWAVGETTT"
     gene            5777..6424
                     /locus_tag="VFB80_05665"
     CDS             5777..6424
                     /locus_tag="VFB80_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33283.1"
                     /translation="MAIVEGIGVVFGLLGAGVLMACCAWLAWWMLEPINRVAGELQGT
                     TRFMLTDVLGLMILLQIGLAVTGQALSRADARDGAVAMYWLLLGLMGVLTLVLWAASV
                     SVVSRAGITRPVRRIAVMVLCVPGTLIIMVALPALIGFFFASSFFVLIGEADQDMTAA
                     RLALMAGGCVLLSAGAFALRWLAFWTLAGSPGGALLAQIQARLAGSHQPADELLP"
     gene            complement(6421..7218)
                     /locus_tag="VFB80_05670"
     CDS             complement(6421..7218)
                     /locus_tag="VFB80_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN33284.1"
                     /translation="MQITSVQNPRVKAAARLRDRKGRDEQGRIIIDGRREIGLALAGG
                     VEVLELYFFPELCQEVRHQQILETAQRAGAELIEVTPHVMEKLAFGNRVEGAVAVART
                     PRRALADLALPADSLVAVVEGVEKPGNLGAILRTADAAGVAALIVADGGTDLYNPNAI
                     RASLGAIFTVPVCAAASEEALAWLRGEKFRLLASRVDGPLAYTDADFRGRAAIVLGSE
                     AEGLSPLWHAADIAAIRLPLVGRVDSLNLSATAAVLFYEALRQRTKT"
BASE COUNT         1228 a         2355 c         2494 g         1289 t
ORIGIN      
        1 ctttccgcag accgtgaccg gcaagatcca gaaattcaaa atgcgcgatg tgatgaaaga
       61 agagttgggg ctggtggacc agaagacggc gtgactgccg cgcctgatcg tgcatcgacg
      121 ttggctcggc ggaggcgcta agcccgaaat atttatttag tgaacggaat aatagagatc
      181 gctcgcgcat ctaagttatg ggtgcgacct cgagcgcggc agtttggcac cgggacttcg
      241 aatgaggctc gcgtcagcga cctgttcacc gagttctcta agtcttatgg tgctaatgtg
      301 aaaaaagcgc cataaatcga catgcagtaa taggttacgt caacttgttc gagtgacgca
      361 gacaagcgat taaccacccg gttagggcct gttcagcctg gtctggcggg cgtttggaag
      421 atgccgctac gagctgccgt tgggcttggg tccggcgtcc aggacctcgg gcgtagcctg
      481 atcggcgtac tgaccgaagt tgacctggaa gagcttggcc aggtggaggg ccttggtgtc
      541 gtaggcgccg ggcttgttcc aggtgtcccg cggcacgagc atctctgcgg gcacgccggg
      601 gcaagccgtc gggaccgcca ggccgaatac gggctcggtc gtggtggggg ccctggtcag
      661 ctgcccgctg tggatcgcgt cgatgatcgc ccgcgtgtga accagcttga tacggctgcc
      721 cgcgccgtag gagccgccgg tccagcctgt gttgaccagc caggcgctgg cctgacgctg
      781 ctgcattttg tcggccagca tcagggcgta cttcatcgga tgaaagacca gaaacgccgc
      841 cccatagcag gccgagaacg ttgcttcggg gtctttcacg ccgacttcgg tgcccgcgac
      901 cttggcggtg tagccgctga tgaagtggta catcgcctgg gccggcgtca ggcgggcgac
      961 aggcggcaac acgccgaagg catcgcaggt gaggaagatg aggtgttgcg gatgtccgcc
     1021 gacgcacgga accttggcat tgggaataaa gtcgatcgga tagcaggccc gcgtgttctc
     1081 ggtgaggtgc gagtcgccgt aatcgaccat ccgcgtcgcc ggatcgacca cgacgttctc
     1141 cagcaccgtg ccggcccgga tcgcacggaa gatttccggc tctttgtcgg ccgacaggtt
     1201 gatgcacttg gcgtagcagc ctccctcgat gttgaaaacg ccgcggtccg accagcagtg
     1261 ctcgtcgtcg ccgatcaacc gccgccgcgg atcggccgag agggtcgttt tgcccgtacc
     1321 cgagaggccg aagaacagcg agacttcgcc cgcggggccc tcgttggccg agcagtgcat
     1381 cgagagcacg ccccgcttgg gcatcaggta gtgcatcacc gtgaacacgc cctttttcat
     1441 ctcgccggcg tattgcgtgc ccagaatgat cagctcgcgg ctctcaaaat tcagggccac
     1501 gcaggtgtcg ctggacagcc cctcgatctc ggcgtcggcg gggaaggagc cggcgttgta
     1561 gatcgtgtag tcgggctggc cgaaatcgcc cagctcggat tgtttcgccc ggatcagcat
     1621 gttccgcatg aacaacgcgt gatacggccg cgagcagacg acgcggatct tcaggcgata
     1681 ctccgggtcc cagccggcgt agccgtccac gacgtacagc ttggggcagc gggtgagata
     1741 gtcctgggct cgtttgcggc agatgcggaa cgagtgctcg gaaagcggga tattaacgct
     1801 gccccaccag acgtcgcgct cattggcggg gcttctggta atccgcttgt cccgcgggct
     1861 gcggccggtc ttcgctccgg agcgagcgat gatcgccccg ctgctggcca gcgagccgtc
     1921 ctcgccgcgg atcgcgtcct catagaggat cgcgggcgcc ggattgcgga cgacgccgct
     1981 ggcaaagatg ccgtgcgaga gaagttcgat gcgatgttcc gacatcctgg gctcgccttc
     2041 cttcacctca accgaaacgt caacatatcc ttcaccccaa aatccgccta ggcgagtcgc
     2101 cgggccagac tacatgcgga agggattggg cgtgaagcaa aacaccggaa tgagcagcga
     2161 cgcccagccc agcacggtgc ggaatgtccc cagcggcatc tcgtcgtcgg cagtcggcgg
     2221 atgatcggtg cccatgacga gcaccagcag ggccatcagc agccagaacg gcatctggtg
     2281 gtaatagatg gttgtgacgc cgaggtaaat gaacacggcc agcatgaaca gccgggccac
     2341 ccaatgggca gcccgaccaa acagcgtata ggtcacgtgc ccgccgtcga gctggctgac
     2401 cggcatcata ttcagggcgg tcaccagcag cccgacccag ccggccatga gatacggatt
     2461 gagcgcgctg aacggcacgc cgacggcggg gttgtattcg ccgggatgga aacgatcgac
     2521 cagcagccgc atcgccagcg gcaactcgag tgcccattcc gcgggcgggg cgtgcttgag
     2581 gtccagccgg gcaacgccga tccagatgat cggaatggcg acgaccagcc ccgccagcgg
     2641 gccggccagg ccgatgtcga acatctgccg gcggttggcc cgcgagccgt ccatgccgat
     2701 caccgcgccc agcgtgccca gcggcgagat cgggaacggg acaaagaagg gcaggctggc
     2761 ccgcacgcga tggaccaccg tcatcacgaa atgccccatt tcatgcgtca gcagaattcc
     2821 gacgatgcag cccatgtaga gcaggccgtc gggccagttc tgcagcacgg ccaggcgggc
     2881 ggagagcagg aagtcggcct gatacagatc cgccggtccg ttgaaaagaa tgaactgatt
     2941 cggcatccag cccgtcgcgc ctgcgaagaa ggtcgaggcg cacgtcagca ggaacagcag
     3001 cgccggcaag agcacgcgcc ggcggcccag ccgccagcgc cgcggcggcg gattacctgc
     3061 caggtccgcc tgcacgacat ccgaggcagg cgcttgcccg atatccgatg cgggctcctg
     3121 cccgacggca gcctcggggg gcggagaatc cagttgggcg ggagtcgaat cgggcacggt
     3181 accggcgggc tgggttctgt ggagggtggg cgacttccta ttctacgcgg cggcgcgatt
     3241 tggcgaaggc tgctagcctt cgccgccccg caggcgcttg cggatcgcgg ccagccgctc
     3301 ggccagctcg gcctcgaagc cgcgcgggac gggctggtag tactcgcgct ggacacccaa
     3361 gtagtcctgc tcggcaatgc cgccctcggc gtcgtgcgcg tattgatatc cctcgccgtg
     3421 gcccagtcgc ttggcgccgt agtagctgcg gtccttcagg tggaccggca ccggcacgat
     3481 ccgcccttcg cgcacgtcct ggcgggctgc ctcgatggcg acggtcgaag cgttcgactt
     3541 gggcgcgcac gccaggtagg tcaccgcctg ggcgagcggc agctggcact cgggcagccc
     3601 gacgaactcg caggcctgca tcgccgcgac ggccagcggc agcgctgcgg gatcggcgtt
     3661 gccgacgtcc tcgctggcca ggatgatcaa tcggcggcaa aggaaccgca cgtcttcgcc
     3721 cgcttcgagc atccgcgcca gccagtagag cccagcgtcg ggatcgctgc cgcggacgct
     3781 cttgatgaga gcggagataa tgtcgtagtg ctcgtccccc gtgccgtcgt acgcgccggc
     3841 cttgcgctgc accgactcgg cggccagctc gcgggtgaat tcgatggtag tccccacggt
     3901 ccgcgtgagg tccgcatctc ttggagcgag atgactactg agcacgccga tctccagcgc
     3961 cgacagtgct cgccgcgcgt cgccgtccga cgcctgggca aggaactgca gcgcgtcgtc
     4021 gtgcatccgc acgtcgtagt tgcccagccc ccgctctttg tcggccagcg ctcgctggat
     4081 gacgagcttg atctcgtcgg gcgacagcgg ctggaactcg aacacctggc tgcggctgac
     4141 cagcgcgctg atcacggcga agaacggatt gcttgtcgtc gctccgacca gcgtcacaat
     4201 gccttcctcg acgtctggca gcagcgcgtc ctgctgggcc ttgttgaagc ggtggatttc
     4261 gtcgatgaac aggagcgttc gctgcccgcc gccggccagg ccgtcgcggg cctcctccag
     4321 cacctgccgc aactctttca cgccgctggt cacggccgaa agctgcacga accgcttctt
     4381 gctctcggtc gccagcaacc gggccagcgt cgtctttccg gttcccggcg ggccatagaa
     4441 gatgacgctg gtcagccggt ctgccgccag caagcgccgc aggagcttgc catctcccag
     4501 aaagtgctgc tgtccggcga actcggccag cgtgcgcggc cgcatccgcg ccgccagcgg
     4561 ctgcgccttg cgcaagtgct cggcttcggc tttggcaaac aagctggcca tgaactgcaa
     4621 ggatccttaa agcgacgtta ctatgaagtc ggtctcgtat ccttatctta tgcctgcggt
     4681 cgtagcccgg cgatggcgcg gaaacgcatg atcactgtgc tctatgtcgc tggcggtctc
     4741 gcgatagcca ccgtcgctgc catcttgatc tttctcgtcg cccaggtcga agactggagc
     4801 cgcgatctga cgacgaattc tgccgaaacg acgagcgacg ctgccgacga gcggctgcgg
     4861 ccgatcgaga ccgcattgcc gccaacggaa ctggcccggc atgtcgaatc ggcggtcacg
     4921 tcgctgaaga actggcgtca ggtggagcgc aaaggttccg gcggcaacat tgaatttcac
     4981 ctgctccgca cgacgcctgt gctcgggttt caggacgaca ttcgcgtgcg gatcgagccg
     5041 gccggcggcg gagcacgcct gacggcccag agccgttcgc gcgtgggcaa aggcgacttg
     5101 gggcagaatc cgcgcaattt gcgggagttg ctggacgctg tgcgatcgcg attgcgctaa
     5161 cagtatcctg gcaggcatgg aattccttct gtccattttc ggagtgctca gttgttgttt
     5221 ttctccggcg ctggccctgg gttacgtttg ggtggtcacg acctggacgc tgggccccct
     5281 gatcgccgcc gcgcgccagg cgcccgcgcc aaccaggttc ctgctcagcg acctgctatg
     5341 gctgatgatt caattgcaac tggcgatggc cctggtggcc ggagcggtcc cgcgcgattt
     5401 tcccacagag acccgcgtct gggccgcaat tctgctcggg ctgcccacgg ccgcattttg
     5461 gtacgcctgt ctgcacgttg tcgcccaagc cggaattggc ggcacgcttc gccgcggcgc
     5521 tgtgttcgtt ctcctcgtgc cgggaacggt cgtggccatc gtcggcgtgc cgatcctggc
     5581 gatggcagtg ctgtcattga ccaatgccag aaactcgcag ccatacgagc tggagtggac
     5641 gaccgtagcg ctaaccgccg gcgcggcggc gacgtttggc atgcgttggc tggcgcggtg
     5701 ggcggtgggc gaaacgacga cataagggtg gacggcctgg gggcagccgc gcgataatcg
     5761 gcgtttgggg gaacgcatgg ccattgtgga aggcatcggc gtggtctttg gattgctcgg
     5821 cgcgggcgtc ctgatggcct gctgcgcgtg gctggcgtgg tggatgctgg agccgatcaa
     5881 ccgcgtcgcc ggcgagctgc agggcacgac gcggttcatg ctgaccgacg tgctcgggct
     5941 gatgattctg ctgcaaatcg gcctggccgt caccgggcaa gcgctcagcc gcgcggacgc
     6001 gcgggacgga gccgtggcga tgtattggct gctactgggg ctgatgggcg tcctgacgct
     6061 ggtgctgtgg gcggcgagcg tgagcgtcgt ctcgcgagcc ggcatcacgc ggccggtgcg
     6121 tcgaatcgcc gtgatggtgc tgtgcgtgcc agggacgctg atcatcatgg tggcgctccc
     6181 ggccctcatc ggctttttct ttgccagctc gttcttcgtg ctgatcggcg aggcggacca
     6241 ggacatgacc gccgcgaggc tggcgctgat ggccggcggc tgcgtgctcc tgtcggccgg
     6301 cgctttcgct ctgcggtggc tggcgttctg gacgctggcc ggttcgccgg gcggcgcact
     6361 gctcgcgcag attcaagccc gcctggccgg ctcgcatcaa ccggccgacg agctgctgcc
     6421 ctaggttttg gtccgctgcc gcagcgcctc gtagaacagc acggcggccg tggcggaaag
     6481 gttgagactg tccacgcggc cgacgagcgg caggcggatc gccgcgatgt cggcggcatg
     6541 ccagagcggc gaaagtccct cggcttcgct gcccaggacg atcgccgcgc ggccgcgaaa
     6601 gtcggcgtcc gtatacgcga gcgggccatc gactcgcgag gcaaggagcc ggaacttctc
     6661 gccgcgtagc caggcgagag cctcctcgct ggccgctgca cagaccggca cggtaaagat
     6721 cgcgccgagg ctggcgcgga tcgcgttggg gttgtagaga tccgtgccgc cgtccgcgac
     6781 gatcaaggcc gcgacgccgg cggcgtccgc cgtccgcagg atcgccccaa gattgcccgg
     6841 cttctcgacg ccctcgacga cggccactag cgagtcggcc ggcagcgcga gatcggccag
     6901 cgcccgccgc ggcgtccggg cgacggcgac agcgccctcg acgcggttgc cgaacgccaa
     6961 tttttccatg acgtgcggcg tgacctcgat cagctcagca cccgcccgct gtgccgtttc
     7021 caggatctgc tgatggcgaa cttcctggca caactccgga aagaagtaca gttcgagcac
     7081 ttcgactccg ccggccagcg ccaggccgat ctcccggcgg ccgtcgatga tgattcgccc
     7141 ctgctcgtcg cgccccttac ggtcgcggag cctggccgcg gctttcacac gcggattctg
     7201 gacgctggtg atctgcatgg ttcacggccc cggtcctacg agcccaagca cccagcacag
     7261 ccagccgacg ggcgcggcga cgagcacggc gtcgatgatg tccagcacgc cgccgaggcc
     7321 cggcagccag gagctgctgt ccttgcgctg catgtcgcgc ttcagg
//
LOCUS       DASRJB010000205         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_341339, whole genome shotgun sequence.
ACCESSION   DASRJB010000205 DASRJB010000000
VERSION     DASRJB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341339"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VFB80_05675"
     CDS             complement(<1..1234)
                     /locus_tag="VFB80_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33285.1"
                     /translation="MRIASLFQSRRQQPNQAKRPAPRTWRPRVEWLEERLPLAINVEF
                     DFSCDASGFFNEPARREVLQEAANEIVGRLNDTLAELPANSGGFTWTAVFDNPGPGNL
                     ACSGNVVKKSFSLPANTLKIFVAGRTFDTPGPSLARPKLGITETQGDAAWDARVKGRL
                     EPGATASPATDFGPWGGSISFDVTASSKSWFIGLLEKSPIRPLLPDEYDFFSVAQRAI
                     GSLLGFGTTDSWNRFLTDSAFTGSKATEIFGAPVPVTKVATGTFWKDGTLFDGKPVSM
                     DLVFNAGQRVHFTNLDLAALHDLGWEVTYAPNAPAPSQDNSRIAVTGIDGLKKFNPPN
                     DFTERHIHEASFNGLIYRAGIDDDDGLWVDVSGDGGQTFRDRNDFGGGEEAIFTPSLT
                     VHGDRLFMAWPGTDSDHLV"
     gene            complement(1399..>3901)
                     /locus_tag="VFB80_05680"
     CDS             complement(1399..>3901)
                     /locus_tag="VFB80_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33286.1"
                     /translation="ELDLLVMKALEKDRSRRYETASSFAADVERYLADEPVQACPPSA
                     AYRLRKFVRRHRGPVLAASLVVLALLIAIVGTTGGMLRAIVAEGKAVNEGKQKELALK
                     DREAALVKAQDRLFLALVNRARAERRSGRVGRRFNALAAIRQAAQIRVTPELRTEAAA
                     ALVLPDVEVAQEWEAFPEGSLAVAFDANFQRYVRLDDQGGLTVCRLNAGREEVVARLP
                     AHGKPPFGGPWMSADGRFAAYGHGSPGVGQLSEVRVWKLNGAGSAVLLDDRNGKHQAA
                     LAFHPAGRQLAIGHADQSVSVYDLATGQRVHRLAVATAPHHLAFHPHESRLAVAGGSA
                     VQLFDTETGQELPALRHPAAVTWTQSVAWHPAGRRLAVGCNDQKIHVWDTQIGAEIMA
                     PWPSGADGISVNYNAAGDRLMSVDWGGQGLWDAVNGRLLLTIPSYIGPQFSQDDELLG
                     YSTSGTKIQLWRLANGRELRVLRRRNADDRERIQNPVLHGDGRTLAACSEHWLSFFDL
                     ASGEELTALRLPRAEAAFPVFFNPPGDSRSPDQGLAGEYAGGWVTAGRSGLFLWPTRP
                     DPASSPVLSVGPPKPLAEGVASWVAHGASASADRRIVAVPQVDHSIVLNRDHPDRRLV
                     LGPQFDVRFTTVSPDGRWVVTCSHWARSKCALIWDAASGEQVHELPLADSTFARFSPD
                     GRWLMTSTVNLGSRLWEVGVWSEVRRYDYARFAFSPDSRLLAIADVYGTIRLVETTTG
                     REVARLTAPEPMWLHPECFTPDGTRLIAASSGFTSLYVWDLRLLRAELKELGLDWNWP
                     EFKPEGGGWPAPLAGPPREIRAAGAELLEAAEHKD"
BASE COUNT          674 a         1294 c         1272 g          661 t
ORIGIN      
        1 tcaccagatg atcgctgtcc gtcccgggcc aggccatgaa gagcctgtca ccgtgaacag
       61 tgaggctcgg agtgaaaatc gcttcttcgc caccgccgaa gtcgttgcgg tcccgaaagg
      121 tctggccgcc gtctccggat acatcgaccc acagcccgtc gtcgtcgtcg atgccggcgc
      181 ggtagatcag gccgttgaac gatgcttcgt ggatatggcg ctcggtaaag tcattcggcg
      241 gattgaactt tttcagtcca tcaatgccgg tgaccgcgat ccgcgagttg tcctgggacg
      301 gcgccggtgc attcggggca tacgtgacct cccagcccag gtcgtgcaga gcggccaggt
      361 ccagatttgt gaaatggacg cgctggccgg cgttgaaaac caggtccatc gacaccggct
      421 tgccgtcgaa gagcgtcccg tccttccaga aggttcccgt tgcgaccttc gtgacaggaa
      481 ccggcgcgcc gaagatttct gtcgccttgc ttccggtgaa ggcactgtcg gtcaggaacc
      541 ggttccacga gtcggtggtg ccgaaaccca aaaggctgcc aatggcccgc tgggcgacgc
      601 tgaagaagtc gtactcatcg ggcaggagcg gccgaatcgg acttttttcc agcagcccga
      661 tgaaccagct cttgctgctg gccgtcacgt cgaaggagat ggacccgccc catgggccga
      721 aatccgtggc aggcgaggcg gtcgcccccg gctccagccg acccttgacc ctcgcgtccc
      781 aagcggcatc gccttgcgtc tcggtgatgc ccaacttggg gcgagccagg gaagggccgg
      841 gcgtgtcgaa ggtccggccg gcgacgaaga tcttcaacgt gttggccgga agcgagaagc
      901 tcttcttcac aacattgccg ctacacgcca ggttgccggg ccctggattg tcgaagacag
      961 cggtccacgt gaagccgccg ctgttggctg ggagttcggc cagcgtgtca ttcaagcggc
     1021 caacaatctc gttggcggct tcttgaagga cctctctgcg ggccggctcg ttgaaaaagc
     1081 cgctagcgtc gcaactgaaa tcgaactcga cgttgatcgc gagcggcagg cgctcctcca
     1141 accattccac gcgcgggcgc caagtgcggg gtgcgggcct cttggcctgg ttcggctgct
     1201 ggcggcggga ctggaaaagg ctcgcgatgc gcatggtcca cctcgtaaaa tgaaagggcg
     1261 accctgtccg gcgcgcacaa tgagcgccag gctcgtgtcg gggccggtga ggggcttgct
     1321 tggcggcttg tgcctcgccg gccgctttgt tatcggatca acgcggcagc gccgcagcct
     1381 tggctcggcg cgatgccgtt aatctttatg ctctgccgcc tccaggagtt ccgcgcccgc
     1441 ggcgcgaatc tcgcggggag ggccggcgag cggagcaggc cagcctccac cctccggttt
     1501 gaactccggc caattccagt cgagccccag ttccttcagc tcggcgcgaa gcaggcgaag
     1561 gtcccaaacg taaagactgg tgaagccgga actggcggcg atcagtcgcg tcccatccgg
     1621 cgtaaaacac tccggatgaa gccacatcgg ttcgggggcg gtcagccgcg cgacctcgcg
     1681 gccggtcgtt gtctcgacga ggcggatcgt gccatagacg tcggcgatgg ccagcaaacg
     1741 gctgtccggg ctgaaggcga agcgggcgta gtcgtagcgc cgcacctcgc tccagacgcc
     1801 gacctcccac agccggctgc cgaggttgac cgtgctggtc atcaaccagc ggccgtcggg
     1861 gctgaacctg gcgaaggtcg agtccgccag cggcagctcg tgtacctgct cgccgcttgc
     1921 cgcatcccaa atcagggcgc acttggaacg agcccagtga ctgcaagtca cgacccagcg
     1981 cccgtcaggg ctgaccgtcg tgaaccgcac gtcgaattgc ggaccaagga cgaggcggcg
     2041 atcggggtga tcgcgattca gcacgatcga atgatcgact tgcggcaccg ccacgatccg
     2101 gcggtcggcg ctcgcactcg caccgtgggc gacccacgac gctacgccct cggcgagggg
     2161 cttgggcgga ccaacgctca gcactgggga agaagcggga tctggtcggg tgggccacag
     2221 aaacaggccg cttcgcccgg ccgttaccca gccgcccgcg tattctcccg ccagcccctg
     2281 atcgggagag cgggaatcgc caggcgggtt aaagaatacg ggaaacgcgg cttctgcgcg
     2341 cggcagtcgc agcgcggtca gctcctcccc gctggcgaga tcgaagaagc tcaaccaatg
     2401 ttccgagcag gcggccagcg tgcggccatc gccgtgcagg acggggtttt gaatccgctc
     2461 tcggtcgtcg gcattgcggc ggcgaagcac gcgcagctct cgtccattgg ccagccgcca
     2521 cagctgaatc ttcgtgccgc tcgtcgaata accgagcagt tcgtcgtcct ggctgaactg
     2581 cgggccgatg tagctgggta tcgtcagcag caaccgcccg ttgacagcat cccagagccc
     2641 ctgtccgccc caatcgacgc tcatcagccg gtcgccggcg gcgttatagt tcacagaaat
     2701 cccgtccgcg ccgctgggcc acggggccat gatctcggcc ccgatctgcg tgtcccaaac
     2761 gtgaatcttt tggtcgttgc aaccgaccgc caaacggcgg ccggcgggat gccaggccac
     2821 ggattgcgtc caggtaacgg ccgccgggtg acgcagcgcg gggagctcct gaccggtttc
     2881 ggtgtcgaaa agttgcactg cactgccgcc ggcgaccgcc aggcgacttt catgggggtg
     2941 aaacgccaga tgatgcggcg cggtggcgac ggcgaggcgg tgcacgcgtt ggccggtggc
     3001 caggtcgtac acgctgaccg actggtcggc gtggccgatg gccaattggc ggccggctgg
     3061 atggaaggcc agggccgctt gatgcttgcc gttgcggtcg tccagcagca cggcggagcc
     3121 ggcgccgttg agcttccaga cgcgaacctc gctaagctga cccactcccg gcgaaccatg
     3181 tccataggcc gcgaagcggc cgtccgcgct catccacggg ccgccgaacg gcggcttacc
     3241 gtgagcaggc aagcgggcga cgacctcttc tcgcccagcg ttcagccggc agaccgtcaa
     3301 gccgccctga tcgtccaggc gaacgtagcg ctggaaattg gcgtcaaagg cgaccgcgag
     3361 gctgccttca ggaaatgctt cccactcctg cgccacctcc acgtcgggta agaccagcgc
     3421 cgccgcagct tcggtccgca gttccggagt gacccgaatc tgcgcagcct gacgaatcgc
     3481 cgccagggcg ttaaaccgcc ggccgactcg cccgctcctg cgctcggctc gggcccggtt
     3541 caccagcgcc aggaatagcc tgtcctgggc cttcaccagt gcggcttccc gatccttcaa
     3601 ggcgagttcc ttctgctttc cctcattcac ggccttgcct tctgcaacga tggcgcggag
     3661 catgcccccc gttgtcccaa cgatggcaat tagcagcgcc aacaccacca gcgaggccgc
     3721 cagcaccggt ccgcggtggc ggcggacaaa cttgcgcagg cggtatgccg ccgacggcgg
     3781 acacgcctgc accggctcgt cggccaggta gcgttcgaca tctgcggcga aactgctggc
     3841 cgtctcgtaa cgccggctgc ggtctttctc cagcgccttc atcacgagca agtccagctc
     3901 g
//
LOCUS       DASRJB010000206         7085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2880664, whole genome shotgun sequence.
ACCESSION   DASRJB010000206 DASRJB010000000
VERSION     DASRJB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880664"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFB80_05685"
     CDS             <1..871
                     /locus_tag="VFB80_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33287.1"
                     /translation="AGLLLPLVGLLVAFPGPAQNSVEVAFLGRLTATLGSPPQLVVWS
                     MLLFYGWAWLRRVPGSEALVVGLGLFSSLLGRQTFDWSSLTLPQPAVVAAIAGVLIVQ
                     ALRFESTWRALAAGTLLVLAARFAGARFGGESLWFWQWHAPLAAALLLPAVFNDPLAR
                     WLRQLAWRVVPAIALAAAAVYPFAWPGLPEGTLSGFLAVLLLSSVVLWRREREVAPLA
                     AVAGTLAANLLAHVRQLYLLLGQTPLADGLPWLACGLAVVLIALAISLLKMGLWTRAW
                     QWLSRVNVALTKP"
     gene            953..1972
                     /locus_tag="VFB80_05690"
     CDS             953..1972
                     /locus_tag="VFB80_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33288.1"
                     /translation="MPRAAVVLWFVGCWFFILAVQGQDVVVVSSVVNPTIKINKPGQI
                     LDFLGDELKLKTKLGAEETIPAARIVAIETTWTPPHEAARAARTAGRLEEAIAAFREA
                     KAAETRLWAGRQLMAELSGCYLDAGRIEDALGEFLAILHSDPGTRHFDMIPIAWRDMP
                     PNPALEARAAAWLALRSSPPAALLGASWLLSTASRAGALAVIEEHERSSDPRLAGLAA
                     IQLWRTKLAAATLDDARRWQTQLEQMPREVQAAGWYVLGELYVRLDQPQRAALACLKP
                     PLLFPEQRLLAADALLAAGGQLEKMARPLEAAALYRELVRDFPQLPPSIQARSRLSKF
                     GRAGD"
     gene            2009..2782
                     /locus_tag="VFB80_05695"
     CDS             2009..2782
                     /locus_tag="VFB80_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN33289.1"
                     /translation="MVVLLLAAGSGAAQDYAPASAATPAAPPQPAETLTFFQIMFSGG
                     PLGILVMLGLVALSIAALALAIDGVWSLRRSAVNQPQLADEVRGLVAAGELAQAAARC
                     DSQPGLLASVLAAGLAEGGGVWSDVQKALEDSLARETARLHRPVEYLGAIANVAPLVG
                     LLGTLAGMLVAFKQSAGSAAGFGSAQLAGGLYQALIPLVVSLLIAIPSLAALVLLRSR
                     IDQVVAEAAGAALSALAPLRRRDQDGGLPLPPPLVNEDG"
     gene            2915..6004
                     /locus_tag="VFB80_05700"
     CDS             2915..6004
                     /locus_tag="VFB80_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33290.1"
                     /translation="MHIATLVSVLEHPHLAAEHLHRWGVRDLARARQTLEELAETGLT
                     LDLLAAVCDSLAEHLPATPDPDEALTAFRRYLFAVRSPLALAALLERDDSAMPMLLAA
                     LSLGPRWANLLAEDPDAFDLLRQTETQPLDREALAADVLAEVTALADERALAIAIARL
                     RRRHTLRIAYGDLVGGWPLATVMEQLSQLAEALLEAALKAAQRSALAARPLPIDSSRW
                     RATIIALGRLGAGELDYASPLDLLVVYDAAETDAAAFRAVQDVQERAARLLVRYLSDA
                     GGGEAAYQVRLVALPDSTSQASAHAAEDAFIGYDSLGRTWHRQALLKARSVAGDRRLG
                     EVLLARLQNWLFRRYLSPADEAGAQALKRRIVMSATLHQDDWRSPHQARGGLRDLEGT
                     VEFLQLLSGGDEPALRQRSTARAIAELGRCGVISAAERQVLEESHAALRRLEHGLQIV
                     PPQPLTELPADAAVMDAVARRMGRASAGEMADLERQAQSRCWETLRKLLGSAFEREPP
                     PPREVELLLDPAPPPEEVRAALAPFGFNDPAAALATLQSLAAEHVPFLSTRRCRHLLA
                     SILPRLLREISATPNPDRTLDSLALVTGSLGSKGVMWDLFRFHPPSLALYVRLCAASP
                     YLSELLTTNPGMLDELVDSLQLDKLPTRGELQATLDELCRHAADTLSVLHDFKNAQHL
                     RIGVRDILGKEDVDRTHLALADVSEICLGHVAELEWQKLVEKFGQPTLGPGPREGEPC
                     HLVIVGLGKLGGREPNYHSHLDFLFLYEGEGTTRPAGRSRQAQRTANNHFFTQLAQRI
                     GKVLADLTPKGRLYSVEASLRPLGVGGALAMSLADFQEHFASGAAPLWQWQALCQARP
                     VWGEAAVCERVERMIRQMLSERPPPAAGPEELRQARLALERGASVQNLKRGPGGTLDI
                     EFLVQLLQLESAARNPQVLCPNTQAAIPLLAAAGAIPPDLATQLGDSYRFLRRVESGL
                     RLLNTQARHDLPGEGQPLAQLALLLGHGNPGRLREQCLAHMAANRQAFDRITRSSSP"
     gene            6023..6676
                     /gene="cmk"
                     /locus_tag="VFB80_05705"
     CDS             6023..6676
                     /gene="cmk"
                     /locus_tag="VFB80_05705"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZN33291.1"
                     /translation="MIVAIDGPAGAGKSSIARQLAERLDFQFLDTGAMYRAVALAALR
                     RGLGDGDAAAIASLADAASIDFDGTRTLLDGEDVSEAIRTSEVSAAVYLAADNVAVRR
                     RLVELQRQIAGGRDTVTEGRDQGTVAFPNAECKIFLTASPQVRARRRYEELRSRGERM
                     TLEEVLAQQADRDRRDAKRPVGALLKAADAVEVVTDGLTPEQVVERLEAIVRQRIRP"
     gene            6673..>7085
                     /locus_tag="VFB80_05710"
     CDS             6673..>7085
                     /locus_tag="VFB80_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN33292.1"
                     /translation="MNEAPRPTQGSRPRTWFQRAGYEVLRVLAQLVAVWVFGLRVAGR
                     ENWPAEGGGLVCANHQSIFDPPLVGMTCPRRMNYLARDTLFRVPGLKQLIAFLDAIPI
                     DREGGGLAGLKETLRRLKAGELVLIFPEGTRTHDG"
BASE COUNT          999 a         2444 c         2459 g         1183 t
ORIGIN      
        1 cgccggcctg ctgctgccgc tcgtgggcct gctcgtcgcg tttccggggc cggcgcagaa
       61 ctcggtcgag gttgctttcc tcggccggct gaccgccacg ctcggctcgc cgccgcaact
      121 cgtcgtctgg agcatgttgc tcttttacgg ctgggcctgg ctgcggcgcg tgccgggcag
      181 cgaggcgctc gtggtcggac tgggcctgtt cagctcgcta ctgggacgcc agacgtttga
      241 ttggagctcg ctcacgctgc cgcagccggc ggtcgtcgcc gccatcgcgg gggtgctcat
      301 cgtccaggcc ctccgcttcg aatcgacctg gcgagcactg gccgcgggca cgctgctcgt
      361 gctggccgcg cgatttgccg gggcacggtt cggcggcgaa tcgctctggt tctggcagtg
      421 gcacgcgccg ctcgcggcgg ccctgctctt gccggccgta ttcaacgatc cgctcgcgcg
      481 gtggctgcgg caactcgcct ggcgcgtggt cccggcgatc gcgctggccg cggcggccgt
      541 atatccgttc gcctggcctg gcttgccgga agggacgctc agcggctttc tcgcggtgct
      601 gctgctctcg tcggtagtgc tctggcggcg cgaaagggaa gtggccccgt tggcagccgt
      661 ggcgggaacc cttgctgcga atctgctggc ccacgttcgg cagctgtacc tgctgctggg
      721 ccaaacgccg ctggccgacg gtctgccctg gcttgcctgc gggctggctg tcgtcctgat
      781 cgccctggcg atcagcctgc tgaaaatggg cctttggacc cgcgcctggc aatggctcag
      841 ccgcgtgaat gttgcactaa ccaaaccgta gtcgacgctg ccagcgtcga cttgtctgac
      901 aagtcgaatg cactcctgcg cgggcgctgg cagcgtcggc tacgatagtc gtatgccgcg
      961 cgccgctgta gttctgtggt tcgttggttg ttggttcttc atcctggccg tgcaaggcca
     1021 ggacgtcgtg gtcgtgtctt ccgtcgtcaa tccgacgatc aagatcaaca agccaggaca
     1081 gattctcgac ttcctgggcg acgagttgaa gctgaaaacg aagctcggcg cggaggagac
     1141 gattcccgct gcgcggatcg tcgccatcga aacgacctgg acgccgccgc acgaagccgc
     1201 ccgcgcggcg cggacagcgg gccgtctgga ggaagcgatt gcggcattcc gcgaggccaa
     1261 agccgcggaa actcgcctct gggctggccg acaactcatg gctgaactat cgggctgtta
     1321 cctcgacgca ggccggatcg aagacgccct tggcgagttc ctggcaattc tccatagcga
     1381 tccagggacg cggcacttcg acatgatccc catcgcgtgg cgggatatgc cgcccaatcc
     1441 agcgctggag gcccgcgcag cggcctggct cgcgctgcgc agctcgccgc ccgcagcact
     1501 gctcggcgcg agctggttgc tttcgaccgc cagtcgggcg ggcgcgctcg ccgtgatcga
     1561 agagcacgag cgctcgagcg atccgcgcct cgccggcctg gctgcgattc agctctggcg
     1621 gacgaagttg gccgccgcga cactcgacga tgcccgccgc tggcagacgc agctcgaaca
     1681 aatgccgcgc gaagtgcagg ccgccggctg gtacgtcctg ggcgagctct atgtccggct
     1741 cgatcagccg caacgggccg cgctggcctg cctcaagccg ccactgctgt ttcccgagca
     1801 gcgcctgctg gcggcagacg ccctcttggc ggccggcggt caacttgaaa aaatggcccg
     1861 tccgctggaa gcggccgcgc tgtaccgcga gctggtccgc gactttccgc agctgccgcc
     1921 gtcgatccag gctcgatcgc gcctgtccaa attcgggagg gcgggcgact aacctgccgg
     1981 acgcttgcaa tcgcttcacg gcttcactgt ggtcgttctc ctgctggccg cgggaagtgg
     2041 cgccgcgcag gactacgcgc cggcaagtgc cgccaccccg gctgcccccc cgcagccggc
     2101 ggaaacgctg acgtttttcc agatcatgtt ctccggcggg ccgctgggca tcctcgtcat
     2161 gctggggctg gtcgcgctct cgatcgccgc gctggcgctg gcgatcgacg gtgtgtggag
     2221 cctccgccgg tccgcggtca accagccgca attggccgat gaggtccgcg gcctcgtcgc
     2281 cgccggagaa ctcgcccagg ctgctgccag gtgcgattcg cagcctggcc tgctggcgag
     2341 cgtcctcgcg gccggactgg ccgaaggcgg aggcgtctgg agcgacgttc agaaggcctt
     2401 ggaagattcc ctggcccgcg agaccgcccg cttgcaccgc ccggtcgagt acctgggcgc
     2461 gatcgccaac gtcgcgccac tggtcggtct gctcggcacc ctcgccggca tgctggtggc
     2521 attcaagcag tctgcaggct cggcggccgg ttttggctcg gcccagttgg ccggcggcct
     2581 ctatcaggcg ctcatcccgc tggtggtgag cctgctgatt gcgattccgt cgctggctgc
     2641 gctggttctg ctgcgcagtc gcatcgacca ggtcgtggcc gaagcagcgg gtgcggcgct
     2701 aagcgccctc gcgccgctca ggcgccgcga ccaagacggc ggcctgcccc tgccgccccc
     2761 gctggtaaac gaggatggct gagcttgccg cgcgattcgc gagggcgcct cggttatgca
     2821 cggcggagct ttcgactatg attggaaaat cccgcctgct gcgcctctct cgcgctgctg
     2881 gtccgtcgcc ctaactgcat ccggccttca ccgcatgcac attgccacgc tcgttagcgt
     2941 cctggaacat ccgcatctcg cagccgaaca tctgcaccgc tggggcgtgc gcgacctggc
     3001 gcgggcccgg cagacgctgg aagagctggc cgagacgggc ctgacgctcg atctgctggc
     3061 cgcagtgtgc gacagcctgg ccgagcacct gcccgcgact cccgatccgg acgaggcgct
     3121 tacggccttc cgccggtatc tgtttgccgt gcgcagcccg ctggccctgg ccgccttatt
     3181 agagcgcgac gactcggcga tgccgatgct gctggcagcg ctctcgctgg ggccgcggtg
     3241 ggcgaacctg ctggcggaag accccgacgc cttcgacctg ctgcggcaaa ccgagaccca
     3301 accgctggac cgcgaggccc tggccgccga cgtgctggcc gaggtgacgg cactagccga
     3361 cgaacgagcg ctcgccatcg caatcgcccg cttgcggcgg cggcacacat tgcggatcgc
     3421 ctatggcgac ctggtcggcg gctggccgct ggccaccgtg atggagcagc tttcgcagct
     3481 ggccgaggcg ctgctcgagg ccgcgctgaa ggccgcgcag cgaagtgcac tggctgcccg
     3541 gccgctgccc atcgattcga gtcgctggcg ggcgacgatc atcgccttgg ggcggctcgg
     3601 cgccggcgag ctggattacg cctcgccgct ggatttgctc gtggtgtacg acgcggccga
     3661 gaccgatgcg gctgcgtttc gcgcggtgca ggacgtacag gagcgggccg cgaggctgct
     3721 cgtgcgttat ctctcggatg ccggcggcgg cgaagccgcg tatcaggtcc gcctggtggc
     3781 gctgcccgac agcacgtcgc aggcctcggc ccatgcggcg gaagatgcat tcatcggcta
     3841 cgacagcctc ggtcgcacgt ggcaccggca ggcgctgctc aaggcccgct ccgtggcggg
     3901 cgaccgcagg ctgggcgagg tgctgcttgc acggctgcaa aactggctct ttcgccgtta
     3961 cctcagcccg gccgacgagg caggcgctca ggcactgaag cggcggattg tcatgtcggc
     4021 gaccttgcac caggacgact ggcgcagccc gcatcaggcc cgcggcggcc tgcgcgactt
     4081 ggaagggacg gtcgagttct tgcagctgct ttccggcggc gacgagccgg ccctgcgcca
     4141 gcgcagcacc gcccgcgcga tcgccgagct gggccgctgc ggcgttatct ccgcggccga
     4201 gcggcaggtc ttggaggagt cgcacgcggc gctgcgacgg ctggaacacg gcctgcaaat
     4261 cgtgccgccg cagcctctga ccgagctgcc ggccgatgcc gcggtgatgg atgcggtcgc
     4321 gcgccggatg ggccgcgcct cggccggcga aatggcggat ctagagcggc aagcgcaatc
     4381 ccgctgttgg gagacgctcc gcaagctgct cggctcggcg ttcgagcgcg agccgccacc
     4441 gccgcgcgag gtcgagctgc tgctcgaccc cgcgccgccg ccggaggaag tccgcgcggc
     4501 attggccccg ttcggcttta acgatccggc ggcggcgctg gctactcttc agtcgctggc
     4561 cgccgagcac gtgccgttcc tgtccacgcg ccgctgccgg cacctgctgg cctcgatcct
     4621 cccgcggctg ctgcgcgaga ttagcgctac gcccaatccc gaccgtacgc tcgacagcct
     4681 ggcgctggtc accggctcgc tcggcagcaa gggcgtgatg tgggacttgt tccgcttcca
     4741 cccgccgtcg ctggcgctgt acgtgcggtt gtgcgcggcc agcccttatc tgtccgagct
     4801 gctcacgacg aatccgggaa tgctcgacga gctggtcgat tcgctccagc tcgacaagct
     4861 gccgacgcgc ggcgagttgc aggcgacgct ggacgaattg tgccgccatg cggcggacac
     4921 cttgagcgtg ctgcacgact tcaagaatgc ccagcatctg cggatcggcg ttcgcgacat
     4981 tctggggaaa gaagacgtcg accgcaccca cctggcgctg gcggacgtga gcgaaatctg
     5041 cctgggacac gtggccgagc tcgaatggca gaagctggtt gagaaattcg gtcagccgac
     5101 gctgggcccc gggccgcgcg agggtgagcc ctgccacctg gtcatcgtcg gcctgggcaa
     5161 gctgggcgga cgcgagccga actatcacag ccacctcgac tttttgttcc tctacgaagg
     5221 cgagggcacg acgcggcccg ccggccgctc gcgccaggcg cagcgcaccg ccaacaacca
     5281 cttcttcacg cagctcgccc agcggatcgg caaggtgctg gccgacctta cgccgaaggg
     5341 ccggctgtac tcggtagagg catcgctgcg tccgctgggt gtcggcgggg ccctggcaat
     5401 gtcgctggcc gatttccagg agcactttgc cagcggtgca gcgccgctct ggcagtggca
     5461 ggcgctctgc caggcgcggc cggtatgggg cgaggccgcg gtctgcgagc gcgtggagcg
     5521 gatgatccgg caaatgctga gcgagcgccc gccgccggca gccggccctg aggaactccg
     5581 ccaggcgcgc ctcgcactcg agcgcggagc cagcgtgcaa aacctgaaac gcggccccgg
     5641 cggcacgctg gacattgagt ttctcgtgca gttgttgcag ctggaatccg ctgctcgaaa
     5701 tccgcaggtg ctctgcccga acacgcaagc ggcgatcccg ctgctcgcgg cggccggtgc
     5761 gattccgcct gacctcgcga cccagctcgg cgactcgtat cgcttcctcc gccgggtcga
     5821 gtcgggcctg cgattgctca acacgcaggc ccggcacgac ctgccgggcg aagggcagcc
     5881 gctggcccaa ctcgccctgc tgctggggca cggcaatccg gggcggctcc gcgagcagtg
     5941 cctggcccac atggccgcaa accggcaggc tttcgataga atcacacgca gcagttcccc
     6001 ttgattctgc tggtccttcg tcatgatcgt cgccatcgac ggccccgctg gagccggcaa
     6061 gagcagcatc gcccgccagc tggccgagcg gctggacttt cagtttctcg atacgggggc
     6121 gatgtaccgg gccgttgcgc tggcagccct gcggcgaggt ctgggcgacg gcgacgcggc
     6181 ggccatcgcc agcctggccg atgcggcctc gatcgatttt gacggcacgc ggaccctgct
     6241 cgacggcgaa gacgtctccg aggcaatacg gacgagcgag gtttcggcgg ccgtttacct
     6301 ggccgccgat aatgtcgcgg tccgccggcg gctcgtggaa ttgcagcggc agattgccgg
     6361 cggccgcgat accgtcaccg agggccgcga ccagggaacc gtggcctttc cgaatgccga
     6421 gtgcaagatt ttcctcaccg ccagcccgca ggtacgggcc cgccgccgct acgaagagct
     6481 gcggtcccgc ggcgagcgga tgacgctgga agaggtgctg gcccagcagg ccgaccgcga
     6541 ccggcgcgac gccaagcgtc cggtcggcgc gctgctcaag gccgcggacg cggtggaagt
     6601 ggtcaccgac ggactgacgc ccgagcaggt cgtggagcgt ttggaggcga tcgtccgcca
     6661 gaggatccgc ccttgaacga agcgcctcgc cccacccaag gctcgcggcc ccgcacctgg
     6721 ttccagcgcg ccggctacga agttttgcgc gtgctggcgc agctggtcgc ggtctgggtc
     6781 tttgggctcc gcgtggccgg tcgcgagaac tggccggccg aagggggcgg gctcgtctgc
     6841 gcgaatcatc agagcatatt cgacccgccg ctggtcggca tgacttgccc gcggcggatg
     6901 aactacctgg cccgcgacac gctgtttcgc gtgccgggcc tcaaacaatt gatcgccttc
     6961 ctcgacgcga ttcccatcga tcgcgaagga ggcggactgg ccggcctgaa ggagacgctg
     7021 cgacgcctca aggcgggcga gctggtgctg atttttcccg agggcacgcg cacgcacgac
     7081 ggcga
//
LOCUS       DASRJB010000207        10275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_910004, whole genome shotgun sequence.
ACCESSION   DASRJB010000207 DASRJB010000000
VERSION     DASRJB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910004"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VFB80_05715"
     CDS             complement(<1..2079)
                     /locus_tag="VFB80_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HZN33293.1"
                     /translation="MTPAPTIDLGHVARDLHLPPERVQRTVELLDEGNTVPFITRYRK
                     DVTGGLDEEQIREIQFRVGKIRQLAERKATILKTIEAQGKLTPELAEQIQHANTPKRL
                     EDLYLPFKPKKQTLATIARERGLEPLAIEIYEAHESASDMQARAATFISAEKELHSVA
                     EVLAGVGHLIAERFSENAELRGRLRRIFARSGKIVCTKVETPPAAQQESGIGSQEPVA
                     SGQEPAAREGEAPAEPQSEAASQEPGAGSQEPASSASGDEQAVLSTECSVPSTDSAGE
                     APEAESAAPTTDATNADAFVPPSLPTPVSPSDPPADAPPAATPNPESLPTGRLFEPPS
                     APVAAPLAAVLLKKKKKKKKKVIGEHAFKDYYSYTEPLGRVPPHRVLAINRGERAHAI
                     RVKIEADEEALFREADEFLVWPKNPHAEFIRNCVRDSLTRLIIPSLERELRREMTERA
                     EEHAVEVFIRNLRKLLLQPPTRGRRVLAIDPGFKSGSKMAALDEFGNVLGHGMIFVIG
                     KDEEKRKKARTRLADLVKQHNVSVIAIGNGTGCRETEQLVADTLAEELQGQDVAYVVV
                     NEAGASIYSTSPLGREELPQYDALQRGAISIGRRLLDPLSEMVKIHPANLGVGLYQHD
                     IKAKHLKDSLDGVVESCVNFVGVDVNSASPALLRYVSGMNALTARRVYEHRREKGPFK
                     NREELHQVAGF"
     gene            complement(2209..3567)
                     /locus_tag="VFB80_05720"
     CDS             complement(2209..3567)
                     /locus_tag="VFB80_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33294.1"
                     /translation="MLTLLGPRASRRHCDGISRRDMLRIGAIGLGGLTLPQLLALESS
                     EAASNAGGKRHKSLIMIYCCGGPPHQDMYEIKNDAPVEVRGEFKAIPTAVPGIEICEL
                     LPNLARIMDKLVPIRTVVGCKDDHAGYQCYTGHLSQNAPAGGWPHIGSSSSRLQGPVL
                     PGVPPFVSLCYVTQHKPYNEPSAGFLGLGHNAFRPMGEGKDDLVLQGITADRLGDRQA
                     LLSSVDRFRREWDGSRTMDGLDTFTQRAMGILTSSEFFAAMDVSKEDQKTRDRYGFYD
                     PKRPKGDGAPRVPQNLLLARRLIEAGVRVVTINYSFWDWHGQNFKNAKEELPIFDKAV
                     SALVEDLHERGMADDCTVIAWGEFGRTPKINKDAGRDHWPRVSCALMAGGGMKTGQAI
                     GTTTRDGGEPAERPVTFEEVHATLYRNLGINLNSAPRLFDFRGRPQYLVDPSVKPIRE
                     LI"
     gene            complement(3651..5018)
                     /locus_tag="VFB80_05725"
     CDS             complement(3651..5018)
                     /locus_tag="VFB80_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33295.1"
                     /translation="MLTIFGSQTYGGFCDRASRRDFLTIGGMALGGLALSDALRVEAA
                     QGSRSHKAIINVYLPGGPPHIDMWDLKPDAPAEIRGEYRPIATNVPGMEICELFPKLA
                     AMADKFAIIRSIADSDGDHAAYQCMTGRKRGQQPPPGGWPAAGAWMSKLKGNVNQAVP
                     AHASLMYRTGEPRWGDPGDGGFLGMGHAPFRLVGGKDNGMKSDNMVLQGVTLDRLQDR
                     NALRASLDRFRRQADASGSMDGLDQFAQKALGILTSSKLAEALDLSLEDPKVVEKYGV
                     DDPKFERDGAPRMVRNFCIARRLVEAGARYVSMNFIRWDWHGGDGMNFVNSKRDFPLL
                     DSGLSSLLTDLHERGLDKDVSVVVWGEFGRTPKINNGAGRDHWPQVSCALLACGGMRT
                     GQVIGATNRLGEHAIERPVSFQEVWATLYHNLGLNLRAIREFDLRGRPQYLVDDGVEP
                     LKEVI"
     gene            5264..6142
                     /gene="tssD"
                     /locus_tag="VFB80_05730"
     CDS             5264..6142
                     /gene="tssD"
                     /locus_tag="VFB80_05730"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HZN33296.1"
                     /translation="MSRPIAPRPRRLCLEALENRLPLAAFIKIPGVDGESEAQGHKNE
                     IQVLSYSWVVEPPAPASAPKVSEIVVTKLVDSASPVLFMKAATGEHLPNVVLDFEKST
                     GGKQQAYLRYELENAFITSYQLGGSAESVPLESISFQFEKIKVVYKEGKAERGKPGSS
                     PQAFVVRTQDPQARGLDLNGDGTADPLFFSFATLADLNGDGVFALIAPPRTAPAAPAD
                     LDGDGMPDLIRPAGNLSVKADLNRDGEQDAMTPVDFDGDGAIDPLFPLDLNADGLADM
                     LAELHVPSTMPRGRGR"
     gene            6272..8191
                     /gene="speA"
                     /locus_tag="VFB80_05735"
     CDS             6272..8191
                     /gene="speA"
                     /locus_tag="VFB80_05735"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HZN33297.1"
                     /translation="MLKDAVERWTTTDASELYDVPRWGKGYFSVNAKGHLAVHPSKDP
                     NLAIDMHELIERLQMRGLDLPILLRFNGILKDRLREINDAFAQAIKDHDYKGRYICVY
                     PIKVNQQRHVVEQIVTHGKAYGFGLEAGSKPEMLATVAMTDADTPIICNGFKDAEFIE
                     MAMLAQKMGRKVIPVVEKYTELGLILKYAEKVGVRPQIGMRIKLAARGSGRWQSSGGW
                     RSKFGLTVSEILHAVEELKSRDMLDCFKLLHFHLGSQITNIRHVKTAVTEAARAYADL
                     AKRGAGLEYLDIGGGLGVDYDGSQTNFESSVNYSLPEYAADVMHHVQAVCEEAAVKHP
                     TIISESGRAVAAYHSCLVFGTLGVSGQGNGDENLLDIPTDSEQPLHDLVESFKNVTAR
                     NILESYHDAQQAMDVAMTLFSTGYLSLDQRALAENLFWAICRKIKRLTEQLDFVPEEL
                     EGLDSLLSDTYFCNFSLFQSMPDSWAIKQLFPVMPIHRLNERPTRPAVLGDITCDSDG
                     KIDQFIDRRDVKRTLRLHPYNGQPYYLSAFLIGAYQEILGDLHNLFGDTNTVHVDLSA
                     TGEVVIEAMVKGDTVREVLDYVEFEPSDLITRLQTSVETAVRENRLSHQEAGRFLKFY
                     EEALNGYMYLEEPGD"
     gene            8433..9680
                     /locus_tag="VFB80_05740"
     CDS             8433..9680
                     /locus_tag="VFB80_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33298.1"
                     /translation="MPRLSLVCLALALISAPAAAQEWSRFRGPNGMGESEATTIPASW
                     TEKTQNWKITLPGKGHSSPVLWGEKIFLLSADPISAERYVLCINAADGKEIWKRTYPG
                     VKHHLHANSSFASSTPACDANQIYVAWSDPEFTRLMALDHAGNEKWTVNLGPWVSQHG
                     FGSSPMLYDNLVVVNCSQEDSMGTDPRLPKESFIVAVERDTGRIRWRRERTIQSASYS
                     VPCVRKNEAGQDELLCCTQAEGIFALDPKTGNQNWSLPVFTMRTVSSPLPLGGLIFGS
                     TGKGAGGHYVVALRPGAEPKVEYEIRRDAPYVPTPVAKGDLLFLFEDRQGHVTCINIA
                     DGKVVWSKPRVAGMFFASPVRAGDKVFCIDIQGTMVCLAADSTFKELGRTELKEECRS
                     TPAIAGGRMYVRTISHLYSVGGK"
     gene            complement(9659..10138)
                     /locus_tag="VFB80_05745"
     CDS             complement(9659..10138)
                     /locus_tag="VFB80_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33299.1"
                     /translation="MQVPLVLIDERRRDEYFRTGYAPLAPGESIAEHKREWRTFVSLL
                     DIALSQRWTQGIGEGDFFLDLDVVADRFVCVEVSAEEMLCPALLRCVHNLVAAFPRAY
                     SVDICDSWGYLKTKDRADYPHFNVFVEREQILVFTESAELLGKLGLSGSAVYFPPTE"
     gene            complement(10160..>10275)
                     /locus_tag="VFB80_05750"
     CDS             complement(10160..>10275)
                     /locus_tag="VFB80_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33300.1"
                     /translation="TILHLVGLDHEALTYHYNGRDMNLTDVHGHVIREVLA"
BASE COUNT         1796 a         3347 c         3278 g         1854 t
ORIGIN      
        1 gaagccggca acctggtgca gctcttcgcg atttttgaag gggccttttt cgcggcgatg
       61 ctcatagacg cggcgagcgg tgagggcgtt catgcccgag acgtagcgga gcaaggcggg
      121 gctggctgaa ttgacatcga cgccgacgaa gttcacgcac gattcgacca cgccgtcgag
      181 cgagtccttc aggtgcttgg ccttgatgtc gtgctgatag aggccgacgc ccagattcgc
      241 cgggtgaatc ttcaccatct ccgagagcgg gtcgagcagc cggcggccga tcgagatcgc
      301 tccccgctgc agggcgtcgt attgcggcag ctcttcgcgg cccagcgggc tggtcgaata
      361 gatgctcgcg cccgcctcgt tcacgacgac gtaggcgacg tcttgcccct gcaactcctc
      421 ggccagcgtg tcggcgacga gctgctccgt ctcgcggcag cccgtgccgt tgccgatggc
      481 gatcaccgag acgttgtgct gtttgaccag gtcggccagg cgggtgcggg ccttcttgcg
      541 cttctcttcg tccttgccga tcacgaagat catgccgtgg cccagcacgt tgccgaactc
      601 gtcgagggcc gccatcttgc tgccgctctt gaagcccggg tcgatcgcca gcacgcggcg
      661 gccgcgggtc ggcggctgca agagcagctt ccgcaggttg cggatgaaga cctcgacggc
      721 gtgctcttcg gcccgctcgg tcatctcgcg gcggagttcg cgctccaggc tggggatgat
      781 cagccgggtg agcgaatctc gaacgcagtt gcggatgaac tcggcgtgcg gattcttcgg
      841 ccagacgaga aactcgtcgg cttcgcggaa gagggcctct tcgtcggcct cgatcttcac
      901 gcgaatcgcg tgcgctcgct cgccgcggtt gatcgccagc acacggtgcg gcggcacgcg
      961 gcccagcggc tccgtgtagc tgtagtagtc tttgaaggcg tgctcgccga tgaccttctt
     1021 ttttttcttc tttttcttct tcagcagcac ggcggccagc ggcgcagcga ccggcgcgga
     1081 aggcggctcg aagaggcggc cggtggggag cgactctggg ttgggagttg ctgcaggtgg
     1141 ggcgtcggct ggcgggtcgg acggagagac gggggtaggg agagacggag ggacgaaggc
     1201 gtcggcgttt gtcgcatcag tcgtcggcgc tgccgattca gcctcggggg cttcgccggc
     1261 tgaatcggta cttggtactg agcactcagt actgagtaca gcctgctcat cacccgacgc
     1321 cgaggacgcc ggctcctggc tcccggctcc tggctcctgg ctcgccgctt ccgactgcgg
     1381 ctcggcggga gcctcgccct cccgtgcggc tggctcctgg ccactggcca ctggctcctg
     1441 actcccgatt cctgattcct gctgcgctgc cggcggcgtt tccactttcg tgcagacgat
     1501 cttgccgctt ctcgcaaaaa tccgccgcag gcggccgcgg agctcggcgt tttcgctgaa
     1561 ccgctcggcg atcaaatggc ccacgccggc gagcacttcc gcgaccgagt gcagctcctt
     1621 ctcggccgaa atgaacgttg ccgcgcgggc ctgcatgtcg ctagccgatt cgtgcgcttc
     1681 gtagatttcg atcgccagcg gctcgaggcc gcgctcgcgg gcgatcgtgg ccagcgtctg
     1741 cttcttcggc ttgaagggca ggtacaagtc ttccagccgc ttgggagtgt tcgcgtgctg
     1801 gatttgctca gccagttcgg gcgtgagctt gccttgggcc tcgatcgtct tgaggatcgt
     1861 cgccttccgc tcggcgagct ggcgaatctt gccgacgcga aactgaatct cgcgaatctg
     1921 ttcttcgtcg aggccgcccg tcacgtcctt gcggtagcgg gtgatgaagg ggacggtgtt
     1981 gccttcatcg agcagctcga cggtccgctg cacgcgctcc ggcggcaagt gcaaatcgcg
     2041 cgcgacgtga cccaagtcga tcgtcggagc tggcgtcatg cgaaaaacca gacctgcgac
     2101 aggaaaccag cggccgcgcg caccgctcga ttagcatcgg gcgaatctaa cgtaatgccc
     2161 gattttgtca agggttacgg cgattcgcgc gctctgcgcg gcctgcatct agatcagctc
     2221 gcggatcggc ttcacgctcg gatcgaccag gtactgtggg cggccgcgga agtcgaaaag
     2281 ccgcggggcg gaattcaggt tgatgccgag gttgcggtag agcgtggcgt gaacctcttc
     2341 gaaggtcacg ggccgctcgg cgggctcgcc gccatcgcgg gtcgtcgtgc cgatggcctg
     2401 gccggtcttc atgccgccgc cggccatgag cgcgcatgaa acccgcggcc agtgatcgcg
     2461 gccggcgtcc ttgttgatct tcggcgtgcg gccgaattcg ccccaggcga tgaccgtgca
     2521 atcgtccgcc atgccccgct cgtgcaaatc ctcgaccagc gcgctcaccg ccttgtcgaa
     2581 gatcggcagc tcttccttgg cgttcttgaa gttctgaccg tgccagtccc agaagctgta
     2641 gttgatcgtc accacgcgga cacccgcctc gatcaaccgg cgggccagca gcaggttctg
     2701 cggcacgcgc ggcgcgccat cccccttcgg ccgcttgggg tcgtagaagc catagcggtc
     2761 gcgggtcttt tggtcttctt tcgagacgtc catcgcggcg aagaactcgg acgacgtgag
     2821 aatgcccatc gcccgctgcg tgaacgtgtc gaggccgtcc atcgtgcggc tgccgtccca
     2881 ttcgcggcgg aacctatcga ccgacgagag cagcgcctgg cgatcgccca gccggtcagc
     2941 cgtaatgccc tgcagcacca gatcgtcctt gccttcgccc atcgggcgaa aggcgttgtg
     3001 gcccaggccc aagaagcccg cgctcggctc gttgtagggc ttgtgctgcg tgacatagca
     3061 caggctcacg aacggcggta cgccgggcag gaccggcccc tgcaatcgcg acgacgagct
     3121 gccgatatgc ggccagccgc cggcgggggc gttctggctg agatggccgg tgtagcactg
     3181 gtagccggcg tggtcgtcct tgcagccgac gacggtgcgg atcgggacga gcttgtccat
     3241 gatccgcgcc aagttcggca gcagctcgca gatctcgatg cccggcacgg cggtgggaat
     3301 cgccttgaac tcgccgcgga cttcgaccgg agcgtcgttc ttgatctcgt acatgtcctg
     3361 gtgcggcggg ccgccgcagc agtagatcat gatgagcgac ttgtggcgct tgccgccggc
     3421 gttcgacgca gcctctgagc tttcgagcgc gagcaattgc ggcagcgtca gcccgccgag
     3481 gccaatcgcg ccgatgcgga gcatgtcgcg acgcgagatg ccgtcgcagt gccggcggga
     3541 ggccctaggg ccgaggaggg taagcatggt agtaggcaca ctccgtgtgc cgtcgttggc
     3601 aagtgaataa tcgattttcg aatgacggca cacggagtgt gcctgctact ttagatcact
     3661 tccttcagtg gttcgacgcc gtcatcaacc aggtattgcg ggcggccgcg gaggtcgaac
     3721 tcgcggatgg cccgcaggtt gagtccgagg ttgtggtaca gcgtggccca gacttcctgg
     3781 aagctgacgg gccgctcgat ggcgtgctcg cccaggcggt tggtcgcgcc gatcacctgt
     3841 ccggtccgca tgccgccgca ggcgagcagc gcgcacgaaa cctgcggcca gtgatcgcgg
     3901 cccgcgccgt tgttgatctt cggcgtgcgg ccgaattcgc cccagacgac gacggaaacg
     3961 tccttatcga ggccgcgctc gtggaggtcg gtcagcagcg acgaaaggcc gctgtcgagc
     4021 agcgggaagt ctcgcttgga gttgacgaag ttcatgccgt cgccgccgtg ccagtcccag
     4081 cggatgaagt tcatcgagac gtagcgtgcg cccgcttcaa ccaggcggcg ggcgatgcag
     4141 aagttgcgca ccatccgcgg cgcgccgtcg cgctcgaact tgggatcatc gacgccatac
     4201 ttctcgacga ccttgggatc ttccagtgac aggtccagcg cttcggccag cttcgaggaa
     4261 gtgaggatgc ccagggcctt ctgggcgaac tggtcgaggc cgtccatcga gccgctggcg
     4321 tcggcctggc ggcggaatcg gtcgaggctg gcccgcaggg cgttgcggtc ttgcaggcgg
     4381 tcgagcgtca cgccctgcag aaccatgttg tcggacttca tgccgttgtc tttgccgccg
     4441 acgaggcgga acggcgcgtg ccccatgccg aggaagccgc cgtcgccggg atcgccccac
     4501 cgtggctcgc ccgtgcggta catcaggctg gcgtgggccg gcaccgcctg gttcacattc
     4561 cccttcagct tgctcatcca cgctcccgca gccggccagc cgccgggcgg cggctgctgc
     4621 ccgcgcttgc ggccggtcat gcactgatag gcggcgtggt cgccgtcgct gtcggcgatc
     4681 gagcggatga ttgcgaactt gtcggccatc gcggccagct tggggaacag ctcgcagatt
     4741 tccatgcccg gcacgttggt cgcgatcggc cggtactcgc cgcgaatctc ggccggggca
     4801 tccggcttga ggtcccacat gtcgatgtgg ggcggtccgc cggggagata gacgttgatg
     4861 atcgctttgt gcgagcgcga cccctgggct gcttcaaccc gcagcgcgtc cgacagcgcc
     4921 aggccgccca gcgccatgcc gccgatcgtg aggaaatccc gccggctggc tctgtcgcag
     4981 aatccgccgt aggtctggct gccaaaaatc gtcagcatgg cgcactcctg aacgtggccg
     5041 acgccggggc atcggcactc cgcatcaact ctcctttatc ggttcaaatt gtgccgcgcc
     5101 gtgagccaag aagcaagaca tccggcggga aacgccacca tgccgcgcgc cgtattggac
     5161 ggtgcttccc gctgcggcat acaatcggtg ctccatggcc gccggcggcc gcgcacggcg
     5221 gctcgactga ccagtttctc gcctcaagcc tggagataaa gtcatgtcgc gcccgatcgc
     5281 tccccgtccg cgtcgcctct gcctggaagc gctggagaac cgcctgccgc tggctgcctt
     5341 tatcaagatt cctggtgtcg atggcgagtc ggaggcccag ggccacaaga acgagattca
     5401 ggtgctctcc tacagctggg tcgttgagcc gcccgccccg gctagcgcgc cgaaggtcag
     5461 cgagatcgtc gtgacgaagc tggtggacag cgcgtcgccg gtactgttta tgaaggcggc
     5521 cactggcgaa catcttccca acgtcgtcct cgatttcgag aagtccacgg ggggcaagca
     5581 gcaggcttat ttgcgatacg aactcgagaa cgcgttcatc acgagctacc agttgggcgg
     5641 ttcggcggag agcgtcccgc tggaatcgat cagcttccaa ttcgaaaaga tcaaggtcgt
     5701 ctataaggag ggaaaggccg agcggggcaa acccgggtcg tcgccgcaag cctttgtcgt
     5761 ccgcacgcag gatccacaag cccgcgggct cgacctgaac ggcgacggca cggccgatcc
     5821 gctctttttc agcttcgcca cgctggccga cctcaacgga gatggcgtat ttgcgctgat
     5881 cgctcccccg cgcaccgcgc ccgcggcacc cgccgacctg gacggcgacg gtatgcccga
     5941 cctgattcgc cccgccggga atctgtcggt gaaggccgat ttgaatcgag acggcgaaca
     6001 ggatgcgatg acgcccgtcg atttcgacgg cgatggcgcc atcgacccgc tgttcccgct
     6061 cgatctcaac gccgacggac tggccgacat gctggccgaa ctgcacgtgc cgagcacgat
     6121 gccgcgcggg cgaggacggt agcccgcggc ggcggcgcgc agaaagaaat taatgctcag
     6181 cggcaaactc tcccttgtcg cgggcccagc atccgtataa tggtgagttt ttacggattg
     6241 cctgccgccg ctggcacagg aacatcgcaa gatgcttaaa gacgccgtgg aacgctggac
     6301 cacgactgac gccagcgaat tgtacgacgt gccgcggtgg ggcaagggct acttctccgt
     6361 caacgccaag ggacatctcg cggtccatcc cagcaaggac ccgaacctgg ccatcgacat
     6421 gcacgagctg atcgagcggc tccagatgcg gggcctcgat ctgccgatcc tgctgcggtt
     6481 caacggcatc ctcaaggacc gtctccgcga gatcaacgac gccttcgccc aggcgatcaa
     6541 ggaccacgac tacaaaggcc gctacatctg cgtctatccg atcaaggtca accagcagcg
     6601 gcacgtcgtc gagcagattg tcacccacgg caaggcctat ggcttcggcc tcgaagcggg
     6661 cagcaagccc gaaatgctcg ccaccgtggc gatgaccgac gccgacacgc cgatcatctg
     6721 caacggcttc aaggacgccg agttcatcga gatggcgatg ctggcccaga agatgggccg
     6781 caaggtcatt ccggtcgtcg agaaatacac cgagctgggg ctgatcctca aatatgccga
     6841 gaaggtcggc gtccgcccgc agatcggcat gcggatcaag ctcgccgccc gcggcagcgg
     6901 ccgctggcaa tcgtccggcg gctggcggag caagttcggc ctgaccgtca gcgaaatcct
     6961 gcacgcggtc gaggagctga agagccgcga catgctcgac tgcttcaagc tgctgcactt
     7021 tcacctgggc agccagatca cgaacatccg ccacgtgaag accgcggtga ctgaggccgc
     7081 gcgggcctat gccgacctgg cgaagcgcgg cgcggggctc gaatatctcg acatcggcgg
     7141 cggcctgggc gtcgattacg acggctcgca gaccaacttc gaatcgagcg tcaattattc
     7201 gctccccgaa tacgccgccg acgtgatgca ccacgtgcag gccgtctgcg aagaggccgc
     7261 ggtcaagcac cccacgatca tcagcgagag cggccgggcc gtcgccgcct accacagttg
     7321 cctcgtcttc ggcacgctcg gcgtctccgg ccagggcaac ggcgacgaga acctgctcga
     7381 cattccgacc gattccgagc agccgctgca cgacctggtc gagtcgttca aaaacgtcac
     7441 ggcccgcaat atcctggaga gctatcacga cgcccagcag gcaatggacg tcgccatgac
     7501 gctcttctcc acgggctatc tctcgctcga ccagcgggcc ctcgccgaga atctgttctg
     7561 ggccatctgc cgcaagatca agcgtctgac cgagcagctc gacttcgtcc cggaagagct
     7621 ggagggcctc gactcgctcc tctccgacac gtacttctgc aacttctcgc tgttccagtc
     7681 gatgcccgac agttgggcca tcaagcagct ctttcccgtg atgccgatcc accggctgaa
     7741 cgagcggccc acgcggcccg ccgtgctggg cgacatcacc tgcgacagcg acggcaagat
     7801 cgaccagttc atcgatcgcc gcgacgtcaa gcgcacgctg cggctgcatc cctacaacgg
     7861 gcagccgtac tacctgtccg cgttcctgat cggggcctat caggaaattc tcggcgactt
     7921 gcacaacctg ttcggcgaca ccaacacggt gcacgtcgat ctcagcgcca ccggcgaggt
     7981 cgtcatcgag gccatggtca agggcgatac cgtccgcgaa gtgctggact acgtcgagtt
     8041 cgagcccagc gacctcatca cccgcctgca gaccagcgtc gaaaccgccg tccgcgaaaa
     8101 ccgcctctcc caccaggaag ccggccgctt cctcaagttc tacgaagaag ccctcaacgg
     8161 ctacatgtat ctcgaagagc cgggggacta agcgggcagg cagaggatta agattaggat
     8221 gagcgagatt acgatcgcgt agcagcgtcc taatcttaat cctaatcctg ctcttaatcc
     8281 ccttcctaat cgtactcttc ctcttgctcc ttctctccgc cggctccggc caccggcagc
     8341 gtcggcagaa ggttgcctcg cccggaagcc tgggttacga tcaaaccgtt cggtccaccc
     8401 gcccgttctc ccgcctggtg agggtcctgc ccatgccccg tttgtcgctc gtttgcctgg
     8461 ctctggcact catttcggct ccggccgccg cccaggaatg gagtcgcttt cgcggaccta
     8521 acggcatggg cgaaagcgag gcgactacga tcccggccag ctggaccgag aagacccaga
     8581 actggaagat cacgctgccc gggaaggggc acagctcgcc cgtgctgtgg ggcgagaaga
     8641 tcttcttgct cagcgccgac ccgataagcg ccgagcggta cgtgctgtgc atcaatgccg
     8701 ccgacggcaa ggaaatctgg aagcggacct atcccggcgt aaagcaccac ctgcatgcca
     8761 acagcagctt tgcctcctcg acgccggcct gcgacgccaa ccagatttac gtcgcctggt
     8821 cggatccgga gttcacccgg ctgatggccc tggaccacgc cggcaacgag aagtggacgg
     8881 tgaatctcgg accctgggtt agccagcacg gcttcggcag ctcgccgatg ctctatgaca
     8941 acctcgttgt cgtgaactgc tcgcaggaag atagcatggg caccgacccg cggctgccca
     9001 aggagagctt catcgtcgcc gtcgagcgcg acacgggcag aattcgctgg cgcagagagc
     9061 ggacaatcca gtcagcgtcg tactcggtgc cctgcgtccg caagaacgaa gccgggcagg
     9121 acgagctgct ctgctgcacg caggccgagg gcatttttgc cctcgatccc aaaaccggca
     9181 accagaactg gtcgctgccg gtcttcacga tgcggacggt cagctcgccg ctgccgctgg
     9241 gcggcctgat tttcggctcg acgggcaaag gcgccggcgg acattatgtc gtcgccttgc
     9301 ggccgggcgc cgagccgaag gtggagtacg aaatccgccg cgacgcgccc tacgtgccga
     9361 cgccggtggc gaagggcgat ttgctctttc tgtttgagga tcgccaaggc catgtgactt
     9421 gcatcaacat cgccgacgga aaggtggtct ggtcgaagcc ccgtgtggcc ggcatgttct
     9481 tcgcctcgcc cgtgcgggcc ggggacaagg tgttctgcat cgatatccag ggcaccatgg
     9541 tctgcctcgc cgccgactcg accttcaaag agctcggccg caccgagctg aaggaggaat
     9601 gccgcagcac gcccgccatc gccggcggcc ggatgtacgt gcggacgatc tcccacctct
     9661 attccgtggg cgggaaatag actgccgaac ccgacagacc gagcttcccg agcaattcag
     9721 ccgactcggt gaaaaccagg atctgttccc gctcgacaaa aacattgaaa tgcggatagt
     9781 cagcccgatc cttggttttc aggtagcccc aactgtcgca aatatcgacc gagtaggctc
     9841 taggaaacgc cgccacgaga ttgtggacgc accttaacag ggcggggcac aacatctcct
     9901 cagcggatac ctcaacgcaa acgaaacgat ccgcgacgac gtcgagatcc aggaagaaat
     9961 ccccttcacc aattccctgc gtccagcgct gggacaaggc gatgtcgagc agacttacaa
    10021 aagttcgcca ctcgcgctta tgctctgcga tgctctcgcc aggcgctagc ggcgcatagc
    10081 ctgtccggaa atactcatcg cgccggcgct cgtcaatcaa gaccagtggc acttgcatgc
    10141 tcaacgtccc gcacggcgac taggccagca cttcccggat gacgtgcccg tggacgtcgg
    10201 tcagattcat gtcgcggcca ttgtagtgat aggtcagcgc ttcgtggtcg aggccgacga
    10261 ggtgcaggat cgtcg
//
LOCUS       DASRJB010000208         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1187102, whole genome shotgun sequence.
ACCESSION   DASRJB010000208 DASRJB010000000
VERSION     DASRJB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187102"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFB80_05755"
     CDS             <1..294
                     /locus_tag="VFB80_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33301.1"
                     /translation="LARRDAVLAAIAAERFRLRTGKQPSQLEALVPEFQADVPRDPFT
                     RGFLKLIADEKSLAIYSVGVDGKDDRGSESSRLNEPDIVVRLKAQANSQLQPQ"
     gene            complement(314..1597)
                     /locus_tag="VFB80_05760"
     CDS             complement(314..1597)
                     /locus_tag="VFB80_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33302.1"
                     /translation="MAQDPPEPGASGNADNVPFIGKSDPQGNPVRLAKSTGHVSNYTE
                     EKVPAYKLPDPLVMASGKRVETAEAWWKERRPEILKFYETEIYGRIPENAPRVAWEVI
                     ESGTSVRDGAATLKRVVGRMGDKADGPKMNLTVYTPAKATGPTPLLLSLTFSFGGARG
                     GGPGKAIPPKTKTPGPGFDSIGEVLSRGWSFASLGYTDIQPDQPGRWTQGVIGLTLKE
                     GQTQPAPDEWGTISAWAWGISRSIDYFETDKSVNAKQIAITGASRLGKTVIWASARDE
                     RVAAVFSVVPGEMGASLIRRDWGETLDDMAQNFGYQFSGNLQKWVGRWNELPVDQHML
                     LALSAPRPVYVNGGLSDQWSDPKGEFLALAAAGPVYKLLGKPDLGATELPSLDQPITT
                     GTLAFHYHSRGHTVVPADWTSFLDFAQRHFAAGKN"
     gene            complement(1656..3539)
                     /locus_tag="VFB80_05765"
     CDS             complement(1656..3539)
                     /locus_tag="VFB80_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN33303.1"
                     /translation="MRVLLLLAATVVFVNLVAPALAQAPRERVNAEMVAKIKEEGMQR
                     SKVMESISFLTDVHGPRLTGSPITRRAGDWAKGRLTEWGLENARLESWGTFGRGWTLE
                     GFTANLVEPDFYPLIAFPKAWSPSMPTAVRGTPIYFDAKTEKDLDSFRGKLHRAIVLI
                     SPPREVKAQFEPQAARQSDERLLALANSDGSATGFRGRGGPGAPGGLPIPGGNPSAAS
                     GARGSSGGQPPMAPGGSSAASQRSSQQVQQKKWDMIYDEGAAVVLEPGRGDGGTMFVA
                     SATMPSRAAGADRGQPNTNPFSRGPRPWAVDAPPIVPQVVLAVEHYNRLVRMIEKGSP
                     VEVEIDLASRYHTDDPTSFNVLAEIPGSDLKDEVVMLGGHFDSWHSGTGATDNAVGCG
                     VALEAVRILQAIGAKPRRTIRIALWTGEEQGLLGSQAYVAQHFGRRVGGGFGTRGGGP
                     RGGGGGGGDGQPGGGGQRGGGGPPARYELKPAHEKFCGYFNLDNGTGKIRGVYLQGNE
                     AVRPIFRSWLSPFLEMGASTITQNNTGGTDHLSFDGVGLPGFQFIQDPLEYDTRTHHS
                     SMDVFERIQEDDVKQASIIMASFVYHAAMRDEKLPRKPLVGEIVKAESEKKAEEKKTE
                     ESK"
     gene            3896..4138
                     /locus_tag="VFB80_05770"
     CDS             3896..4138
                     /locus_tag="VFB80_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33304.1"
                     /translation="MPNSETLASWLMLACLSMFIAVSIVAGCTLSLYMAEARHYQKLT
                     GEPSTVFDEIWEDFRPKQQIVKRRSSAAAAPSPPPR"
     gene            complement(4089..4757)
                     /gene="rpe"
                     /locus_tag="VFB80_05775"
     CDS             complement(4089..4757)
                     /gene="rpe"
                     /locus_tag="VFB80_05775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZN33305.1"
                     /translation="MANLIKIAPSILAADFARLGEQVAEAEAAGADRIHIDVMDGHFV
                     PNISIGVPVVKSLRRVTQLPLETHLMIEEPDRYLEAFANAGSDTLIVHWEGNANLHRT
                     VQEIHRLGKRAGVAINPATPATVLDEILPDLDLVLVMTVNPGFGGQEFITGTLPKIAQ
                     VQQMIEAAGRDCELEIDGGVDPQSAPRGVAAGANVLVAGSSIFGGKLTVAAAMERLRQ
                     SCGA"
     gene            complement(4750..6411)
                     /gene="pgi"
                     /locus_tag="VFB80_05780"
     CDS             complement(4750..6411)
                     /gene="pgi"
                     /locus_tag="VFB80_05780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZN33306.1"
                     /translation="MAATIPPLTARPAWKLLGTHCQQLKPVHLRKLFADDPGRGTRLA
                     LDAAGIYFDYSKNRITDETLKLLVQLAGESNLRGRIDAMFAGERINTTEDRAVLHVAL
                     RAPEGATFVDAGEKSPENVVPQVHAMLDKMAKFADEIRKGDYLGHTGRPIKNIINIGI
                     GGSDLGPVMAFEALRPFSQRNLTCRFVSNIDATDLVEATDDLDPAETLFIVASKTFTT
                     QETLANARTARNWCLAKLKDEAAIGRHFVAVSANAAAVKEFNIQTRFDLWDWVGGRYS
                     LDSAIGLSLMIAIGPQRFREMLAGFHELDEHFRTAPFEQNLPVLHGLLTLWNTNFLGA
                     TSVAILPYDQYLRRLPAYLQQLTMESNGKSVTFEGQRVAYDTSPIYWGEPGTNGQHSF
                     YQLIHQGTRLVTCDFIGCCLSHNPRGDHHDLLMANVFAQAEALAFGKTADELRAENVP
                     PALVAHRTFEGNRPSNVLLLDRLTPAALGKLIAFYEHSVFVQGAIWNINSFDQMGVEL
                     GKALARRIAPELSSPDAKLAHDSSTNALIARYRARRSAAAEASRG"
     gene            complement(6435..8738)
                     /locus_tag="VFB80_05785"
     CDS             complement(6435..8738)
                     /locus_tag="VFB80_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33307.1"
                     /translation="MPSVNSYTRCGLLLICGWLAGCAHIRIPAIDPSGERLFSGTTTF
                     ESHGLFHHHRHAQPPAVATTPPVLPPQPVVAAPPCNPPLAAAPPPPVIAVPVAPAVPI
                     VPVACGPQQTVIPSPPKAAGPICAHPASYRGPELQVTPTRIVAPVNSEVILAAGVSAP
                     DGYYVMRQPLEWMLAQDGVGQFVAVGHESPHNLSFLLRDSPQKVATNYARAHTSTISQ
                     TIDRGTPSPGDDVYLQKGQAWITVTSPTEGTSHVVVWAPKEHNWERRKATATIFWIDA
                     AWRYPPCAVARAGAGRGVALTTVVTRSSGEPLPGWRVRYTVLEGPPAVFSARRNTEEI
                     AITDGAGRATVQLLPGSQEPGITTIGVQVIRPASGRGDLPEMVVGQGTTTVQWTTPGL
                     SVQAVGASSVAADGAIGYRVQVANGGDLVTRGVTLSYEPPPGIAVLNSSPPAQAFGQR
                     LQWRLGDLQPGTAIAVELSCRAAVSAAVRSTFRAASTDGLTAEGTATTEVFANAISVR
                     MTGPDTAPVGSEVKFLIDVTNTGQTALTNLVASDTFEPGLAERGGAQSPAVRPIPQPL
                     APGETYSFALTFIVTQAGRWSHRLDASADGGHRGSARSFVIGTQPVIAPGPAAPPSTA
                     ATPAPRPIPPAATAPPTLGGLKVTASLLANPIRQGATTTCDINITNDRSIPDQDLTLS
                     VQLIGDGVALTRTPVKGGVTASARVGANSIEFMPLREIRAGEVLAPYRLEVQGVKAGR
                     HKIRVTVTSSRSPAGVTAEAELTVLAP"
     gene            complement(8895..>9485)
                     /locus_tag="VFB80_05790"
     CDS             complement(8895..>9485)
                     /locus_tag="VFB80_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33308.1"
                     /translation="GPAPMPLPPTPDPFGDRRKSLEEQFFKDKDRQLIEKMRGELTAL
                     EERKQLAHVSGIVEERVLENLVKAGVKAETLAAVSLIPIVEVAWADGSISPEERDAVL
                     NAAAAQGVKPDTAAYEMLRTWLKTRPDKHVIAAWKDYVKEASKLMPKDTVAAMKKAMI
                     DRATRVAEAAGGFLGMATISQSERKTIDEFAKAFDG"
BASE COUNT         1513 a         3088 c         3212 g         1672 t
ORIGIN      
        1 ttggctcgtc gagatgccgt cttggctgcg atcgccgcgg agcgttttcg cctgagaacc
       61 ggcaaacagc cgtcgcagct ggaggcactc gtccctgagt ttcaggcgga tgtgccacga
      121 gaccccttca caaggggttt cttgaagttg atcgccgacg agaagagcct ggcgatttac
      181 agcgtgggcg tcgatggcaa agatgaccgc ggctccgaaa gctcgcgcct caacgaaccc
      241 gatatcgtcg tgcggctgaa agcacaagcg aactcgcaat tgcagccgca gtgaattccc
      301 gtgcagcgag tttctaattc ttgccagccg caaagtgccg ctgggcgaag tcgaggaagg
      361 atgtccagtc ggcgggaacg acggtgtggc cgcggctgtg gtagtggaac gcgagggtgc
      421 cggtggtgat cggctgatcg agcgacggca attcggtcgc gccgagatcg ggtttgccga
      481 gcagcttata gacggggccg gcggcggcga gggcgaggaa ttcgcccttg gggtcgctcc
      541 attgatcgct caggccgccg ttgacgtaga cgggccgcgg cgcgctgagg gcgaggagca
      601 tgtgctgatc gacgggcagc tcattccagc ggccgaccca cttctgcaga ttgccggaga
      661 actggtagcc aaaattctgg gccatgtcgt cgagcgtctc gccccagtcg cggcggatca
      721 gcgacgcacc catctcgccc ggcaccacgc tgaacacggc cgccacccgc tcgtcgcggg
      781 cactcgccca gatcaccgtc ttgcccagcc gcgaagcgcc ggtgatcgcg atttgcttcg
      841 cgttgacgga cttgtcggtc tcgaaatagt cgatcgaacg gctgatgccc caggcccagg
      901 cgctgatcgt gccccactcg tccggcgctg gctgcgtctg gccctctttg agcgtcaggc
      961 cgatgacgcc ttgcgtccac cgccccggct ggtcgggctg aatgtcggta tagccgagcg
     1021 aagcaaagct ccagccgcgg ctgagcactt cgccgatgga atcgaagcca ggaccgggcg
     1081 tcttggtctt gggcggaatg gctttacccg gtccgccgcc tcgagccccg ccgaagctga
     1141 acgtaaggct gagcagcagc ggcgtcggcc cggtcgcctt ggccggcgta tagaccgtga
     1201 ggttcatctt aggcccgtcg gctttgtcac ccatgcggcc gacgacgcgc ttcagcgtgg
     1261 cagcgccgtc gcggacgctc gtccccgact cgatcacctc ccaggccaca cgcggggcat
     1321 tctccggaat gcggccgtaa atctcggtct cgtagaactt gagaatttcc ggccggcgct
     1381 ccttccacca ggcctcggca gtctcgacgc gcttgccgct ggccattacc agcggatcgg
     1441 gcagcttgta cgcggggacc ttctcctcgg tgtagttcga gacgtgcccg gtggacttcg
     1501 ccagccgcac ggggttgccc tgcgggtcgc tcttgccgat gaacggcacg ttgtcggcgt
     1561 tcccgctcgc tcccggctcg ggtggatcct gagccattgc cgccatgcaa aaagccagca
     1621 gtaccacagg caagaatgcg cgcatcggac ggctcctatt tcgactcctc ggttttcttc
     1681 tcctccgcct tcttctcgct ttccgccttg acgatctcgc cgacgagcgg cttgcggggc
     1741 agcttctcgt cgcgcatggc ggcgtgatag acgaaggagg ccatgatgat tgaggcctgc
     1801 ttcacgtcgt cctcctgaat ccgctcgaag acgtccatgc tggagtggtg cgtgcgggtg
     1861 tcgtattcca gcgggtcctg gatgaactga aagccgggca ggccgacgcc gtcgaacgac
     1921 aggtggtcgg tgccgccggt gttgttctgc gtgatcgtcg aagcgcccat ctcgaggaac
     1981 ggcgagagcc agctgcggaa gatgggccgc acggcttcgt tgccctgcag atagacgccg
     2041 cgaatcttgc ccgtaccgtt gtcgaggttg aagtagccgc agaacttctc gtgggccggc
     2101 ttcaactcgt accgcgccgg agggccgcct ccgccgcgct gcccgccgcc gccgggctgc
     2161 ccgtcgccgc cgccaccgcc gcctccacgc ggtccaccgc cgcgggtgcc aaagccgccg
     2221 ccgacgcggc ggccaaagtg ctgggcgacg taggcctgcg agccgagcag gccctgctcc
     2281 tcgccggtcc agagggcgat gcggatcgtc cgccggggct ttgcaccgat cgcctgcaaa
     2341 atgcggacgg cttccagggc cacgccgcag ccgacggcat tgtcggtcgc cccggtgccc
     2401 gaatgccacg aatcaaaatg gccgccgagc atcacaacct cgtccttcag gtcgctgccc
     2461 ggaatctcgg ccaggacgtt gaagctcgtc ggatcgtcgg tgtgatagcg gctggccagg
     2521 tcgatctcca cttcgaccgg gctgcctttc tcgatcatcc gcaccaggcg gttgtagtgc
     2581 tcgaccgcga ggaccacctg cggcacgatc ggcggggcat cgaccgccca cggccgcggc
     2641 ccgcggctga acggattcgt gttcggctgg ccgcgatcgg cgcccgcggc ccgcgagggc
     2701 atcgtggcgg aagccacaaa catcgttccg ccgtcgccgc ggcccggctc cagcacgacc
     2761 gccgccccct cgtcatagat catgtcccac ttcttctgct gaacctgctg gctcgatcgc
     2821 tggctggccg ccgaactgcc gcccggcgcc atcggcggct gaccgccgga gctgcctcgc
     2881 gcgccgctgg cagcagaagg attgccgccc ggaattggca atccgcctgg agctcccgga
     2941 ccgccgcggc cgcgaaatcc agtggccgag ccgtccgagt tggccagggc cagcaatcgt
     3001 tcgtcggatt gccgcgcggc ctgcggctcg aactgggcct tcacctcccg cggcgggctg
     3061 atgagcacga tcgcgcggtg cagcttgccg cgaaagctgt cgaggtcctt ctcggtcttg
     3121 gcgtcgaaat agatcggcgt cccccggacc gcagtaggca tgctcggcga ccaggcttta
     3181 ggaaacgcaa tcagcggata aaagtccggc tcgacaaggt tggcggtgaa gccctcgagc
     3241 gtccagccgc ggccaaacgt accccacgat tccaggcggg cgttttcgag tccccactcg
     3301 gtcagccgtc ccttggccca atcgccggcc cgccgcgtga tcggcgagcc cgtcagccgc
     3361 ggcccgtgca cgtcggtcag gaagctgatg gactccatga ccttcgaccg ctgcatgcct
     3421 tcttctttga tcttggcgac catctcggcg ttcacgcgct cgcgcggcgc ttgggccagg
     3481 gcgggagcaa ccaggttgac gaaaacaaca gtggcggcaa gcagaagaag tacgcgcatg
     3541 gctgtgtaga gaagggcggc ggaattcacg gctgcgacca gcgccgtgcg gggaagacga
     3601 gcattccgcg gcgaggctgg ggagattatg ttccgccacg cggcagaatg ccaggaatgg
     3661 ccgccgccgc ggaacttctt tgaaaaatag ttatttagta attattcaat tgcctcagca
     3721 cgacccgaac tcgcccaatt cgcgggcaag gttcgcccat tctgcgcgcg gcgttcatgc
     3781 ggctgctgca aactttgcgt cgtaagtcgt tggagcaaac gacgccgccc gatcccccgc
     3841 gttttggcac cggctttgcg tttctcacgc gctagcgggc caaggaaggt caacgatgcc
     3901 aaattcagag acgctggcaa gctggctgat gctggcttgc ctctccatgt tcattgccgt
     3961 gtcgatcgtt gccggttgca cgctgagcct gtatatggcg gaggcccggc attaccagaa
     4021 gctcaccggc gagccgagca cggtgttcga cgagatttgg gaagactttc ggccgaagca
     4081 gcagatcgtc aagcgccgca gctctgccgc agccgctcca tcgccgccgc cacggtgagc
     4141 ttgccgccga agatcgagga gccggcgacg aggacgttcg cgcccgcggc gacgccgcgc
     4201 ggcgcggatt gcggatcgac gccaccgtcg atttccaatt cgcagtcgcg gccggcggcc
     4261 tcgatcattt gctggacctg cgcgatcttg ggcagcgtgc ccgtgatgaa ctcctggccg
     4321 ccgaagccag gattgaccgt catcacgagc accaggtcga gatccggcaa gatctcgtca
     4381 agcactgtcg cgggcgtcgc ggggttgatg gcgacaccgg cccgcttgcc caggcggtgg
     4441 atctcctgca ccgtgcggtg caggttggca ttgccctccc agtgaacgat cagcgtgtcc
     4501 gagccggcgt ttgcgaacgc ctccagatag cggtcgggct cttcgatcat caggtgcgtt
     4561 tcgagcggaa gctgcgtcac gcggcgcagc gacttgacga ccggcacgcc gatcgagatg
     4621 ttcggcacga agtgcccgtc catgacgtcg atgtggatgc ggtccgcgcc tgccgcttcc
     4681 gcctcggcga cctgctcgcc gagccgcgca aagtcggccg ccagaatcga cggggcgatc
     4741 ttgatgaggt tagccacggc tggcctccgc agcggccgat cgccgggcgc gatagcgggc
     4801 gatcagggca ttggtcgagc tgtcgtgggc gagcttcgcg tccgggctcg acaattccgg
     4861 ggcgatccgc ctggcgaggg ccttgcccag ctcgacgccc atctggtcga acgagttgat
     4921 gttccagatc gcgccctgaa cgaacacgct gtgctcgtag aacgcgatca gcttgcccag
     4981 cgcggccggc gtcaggcggt cgagcagcag gacgtttgac gggcgattgc cttcgaacgt
     5041 gcgatgtgcg acgagcgccg gggggacatt ctcggcccgc aattcgtcgg ccgtcttgcc
     5101 gaaggccagg gcctcggcct gagcgaaaac gttggccatc agcagatcgt ggtggtcgcc
     5161 gcgcgggttg tgcgacaggc agcaaccaat gaagtcgcag gtaaccagcc gcgtgccctg
     5221 atggatgagc tggtagaagg agtgctggcc gttggttccc ggctcacccc agtagatcgg
     5281 gctggtgtcg taggccacgc gctgcccttc gaacgtcaca gacttgccgt tgctctccat
     5341 cgtgagctgc tggaggtacg cgggcagccg cctgaggtac tgatcgtagg gcaagatggc
     5401 gacggaggtc gcgccgagga agttcgtgtt ccagagcgtc agtaggccgt gcagcaccgg
     5461 caggttctgc tcgaagggcg cagtgcggaa gtgctcgtcc agctcgtgaa agccggcgag
     5521 catctcgcga aagcgctgcg gcccgatcgc aatcatcagc gacagtccga tagccgaatc
     5581 gagcgagtac cgcccgccga cccagtccca gaggtcgaac ctggtctgga tgttaaattc
     5641 cttgacggct gcggcgttcg ccgagacggc gacgaaatgc cggccgatcg cggcctcgtc
     5701 cttcagcttc gccaggcacc agttgcgggc cgtacgggcg ttggccagtg tttcctgggt
     5761 cgtgaacgtc tttgacgcga cgatgaacag cgtctccgcc ggatccaggt cgtccgtcgc
     5821 ctccacgagg tccgtcgcgt cgatattcga gacgaagcgg caggtgagat ttcgctgact
     5881 gaagggccgc agcgcctcaa acgccatcac gggaccgaga tccgatccgc cgatgccgat
     5941 attgatgatg ttcttgatcg gccggcccgt gtggcctaga tagtcgccct tgcggatctc
     6001 gtcggcgaat tttgccatct tgtcgagcat ggcgtgaacc tgcggcacca cattctcggg
     6061 cgacttttcg ccggcatcga cgaaggtcgc tccctcggga gctcgtaagg cgacatgcag
     6121 gaccgcgcgg tcctcggtcg tgttgatccg ctcaccggcg aacatggcgt cgattcgccc
     6181 acgaaggttc gactcgccgg caagctgcac gagcagcttc aatgtctcgt cggtgatgcg
     6241 attcttgctg tagtcgaaat agatgccggc cgcatccagc gctagccgcg tcccgcggcc
     6301 gggatcgtcg gcgaacagct tccgcaggtg caccggcttc agctgctggc agtgcgtgcc
     6361 caagagcttc caggcagggc gggcggtcag cggcggaatc gtcgcggcca tgcagctagt
     6421 gtagccgaat tcggctacgg cgcgagaacc gtcagctccg cctcagccgt gacgcccgcg
     6481 ggcgagcggc tgctggtgac cgtgacgcgg atcttgtggc gtccggcctt gaccccctgc
     6541 acttcgaggc ggtagggcgc aaggacttcg ccggcccgaa tttcacggag cggcatgaac
     6601 tcgatggaat tagcgccgac tcgggccgag gctgttactc ctcccttgac cggcgtccgc
     6661 gtaagcgcca cgccatcgcc gataagctgc acgctgagcg tcaagtcctg atccgggatg
     6721 ctgcgatcgt tcgtgatgtt gatgtcgcag gtggtcgtcg cgccttggcg gatcggattg
     6781 gccagcagcg aggccgtgac tttcagcccg cccagcgtag gcggcgccgt tgcggctggc
     6841 ggaatcggcc gcggcgcggg agtggcagcc gtggagggcg gcgcggccgg gcccggcgcg
     6901 atcaccggct gcgtgccgat tacgaacgag cgggccgagc cgcggtgacc gccgtcggcc
     6961 gatgcgtcga gccgatgact ccagcggccg gcctgcgtga cgatgaacgt gagggcgaag
     7021 ctatacgtct cgccgggggc cagcggctgc ggaatcggcc gcacggcggg gctctgagcg
     7081 ccgccccgct cggccaggcc gggctcgaac gtgtcgctcg ccaccaggtt ggtaagcgcc
     7141 gtttgcccgg tgttggtcac atcgatcagg aacttgacct cggagccgac cggcgcggtg
     7201 tcggggccgg tcatcctcac ggagatggca ttggcgaaga cttcggtcgt cgccgtgccc
     7261 tcggcggtca agccgtcggt gctcgccgcg cggaacgtgc tgcggaccgc ggcgctgacc
     7321 gccgcccggc aactcagctc gacggcaatg gccgtgcccg gctgcaagtc gcccagccgc
     7381 cactgcaggc gctggccgaa agcctgggcg ggcggcgagc tgttgagcac ggcgatgccc
     7441 ggcggcggct cgtagctcag cgtgacgccg cgagtgacaa ggtcgccgcc atttgcgacc
     7501 tgcacgcgat agccgatcgc gccgtcagcc gcgacgctgc tggcgccgac tgcctgcacc
     7561 gacagaccgg gcgtcgtcca ctgcaccgtg gtcgtgccct gcccgactac catctccggc
     7621 aagtcgccgc ggccgctcgc ggggcggatg acctgtacgc cgatcgtggt gatgcccggc
     7681 tcctgcgatc ccggcagcaa ctgcacagtt gcccggcccg cgccgtcggt gatcgcgatt
     7741 tcctcggtgt tgcggcgggc tgagaagacg gccggcgggc cctccagcac ggtgtatcgc
     7801 acgcgccagc cgggcagcgg ctcgccgctg gagcgcgtga cgacggtcgt cagagccacg
     7861 ccgcggcccg cgccggcgcg ggcgaccgca cacggcggat agcgccaggc ggcgtcgatc
     7921 cagaagatcg tcgcggtggc cttgcggcgt tcccagttgt gttctttcgg ggcccacacg
     7981 acgacgtggc tcgtgccttc cgtcggcgag gtgacggtga tccaagcctg gcccttctgc
     8041 agatagacgt cgtcgcccgg cgacggcgtg ccgcgatcga tggtctgcga aattgtcgac
     8101 gtgtgcgccc gggcgtagtt ggtcgccact ttctgcggcg aatcgcggag caggaacgac
     8161 aggttgtgcg ggctctcgtg gccgaccgcc acgaattgcc ccacgccgtc ctgggccagc
     8221 atccactcca gcggctgccg catcacgtaa taaccgtcgg gtgcggagac gccagccgcg
     8281 agaatcacct cgctgttgac cggcgcgacg atccgcgtgg gcgtgacttg caactctggt
     8341 ccgcgataac tcgccggatg cgcacagatc ggcccggccg ccttcggcgg actgggaatg
     8401 acggtttgct gcgggccgca ggcgacgggg acgatcggca cagcaggcgc aacgggcacg
     8461 gcaataaccg gtgggggagg cgccgcggcg agcggaggat tacacggagg tgctgccacc
     8521 accggctggg gcggcagcac gggcggcgtt gtggccacgg ccggcggctg tgcgtggcga
     8581 tggtgatgga acagtccgtg cgactcgaag gtcgtggtgc cagagaacag ccgctcgccg
     8641 ctcgggtcaa tggccgggat ccggatgtgg gcgcagccgg cgagccagcc gcatatcagc
     8701 agcaaaccgc agcgcgtgta ggaattgacc gagggcatga gacgatggcg gcaagcactt
     8761 gggcgcgctc gcccccgcaa acgtagcatg cgttttggca acttgtggcg aagaggcaac
     8821 gtggagcgtt gctcgcaggc tgcgcgcggt aagttgcgcc ggttatgacg attgcgcggc
     8881 cagcggcccg ggggttagcc gtcgaaggct ttggcgaatt cgtcgatcgt tttccgctcg
     8941 gactgcgaga tggtcgccat gcccaaaaat ccgccggcgg cttcggcgac gcgggtggcc
     9001 cggtcgatca tcgccttctt catcgcggcg accgtatctt tgggcatcag cttcgacgcc
     9061 tctttcacgt aatccttcca ggccgcgatg acgtgcttat cgggcctggt cttgagccag
     9121 gtcctgagca tttcgtaggc cgccgtatcg ggcttcacgc cctgggctgc ggccgcgttc
     9181 agcacggcgt cgcgttcttc gggcgaaatg gagccgtccg cccaggcgac ttccacgatc
     9241 gggatcaagc tcacggccgc cagcgtttcg gccttgacgc cggccttcac cagattctcc
     9301 agcacccgct cttcgacgat gcccgagacg tgcgccagtt gcttcctttc ctcgagcgcc
     9361 gtcaactcgc cgcgcatctt ctcgatgagc tggcggtctt tgtccttgaa gaactgttct
     9421 tccagactct tgcggcgatc gccgaacgga tcgggagtcg gaggcagagg catcggggca
     9481 ggtcc
//
LOCUS       DASRJB010000209        12091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2027847, whole genome shotgun sequence.
ACCESSION   DASRJB010000209 DASRJB010000000
VERSION     DASRJB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027847"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..834)
                     /locus_tag="VFB80_05795"
     CDS             complement(292..834)
                     /locus_tag="VFB80_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN33309.1"
                     /translation="MRCWHAGAGEFWLPAVIGVAAINGLVAVLFFARRPVVSLGAPWQ
                     LASCLPSMIGSGLALRLAPGLTSWPYWAHGLFAAGALLTLVALAWLGRSFGVMPALRQ
                     TVQAGPYALVRHPAYLGELIMTLACFLAGPGWLAASAWVLLVPGVAWRIWAEETLLSA
                     DPHYQAYQQQVRSRLIPGVW"
     gene            1280..2869
                     /locus_tag="VFB80_05800"
     CDS             1280..2869
                     /locus_tag="VFB80_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN33310.1"
                     /translation="MNARALLSLWLIAGALVAGALGQAPAPVKPLGEADLASLLGLGL
                     DDETIVARIKKGGLAFDLTDAAVQKLKSAGASEAVVKALQAAAQANSQPTSAASAITY
                     DQVLQLLGLGLDEESVLKRLAKSPTVFTLDAAQTEALKKAGATPKILEALTGVRAASA
                     GGDVTDLALILDCSGSMSQATTGGETKMTAAKRVLTDLVGKIPAGLNVTFVIYGHEVY
                     GGAEDPRNCQAVKVVRPLAPLDEAGRTALVTTIAALKPTGATPIALSLRTAGEELAKN
                     KSMCGLVLVTDGIESCKGDPAAEAAALVAKLNVSFGVNVVGFGVKPEENAALKEIAVA
                     GKGKYYGAADSKELTDAISTIAKQIQDNAEIVDTTRRALRVLTPKKVQMPPVEEIYIV
                     RNEEADSPIATKIGSVEKFDDYLSIPSSTNKYAVFFQAKDGWAAVPLLRNFSIPDRRV
                     VDVKPESILGMVQVNGTGKTERIYIRKPGDRGQVNPPVQLAEKFGDVMVVPAGKYDIY
                     VGNSLIEEDFEVKPGTLHRLK"
     gene            2884..3564
                     /locus_tag="VFB80_05805"
     CDS             2884..3564
                     /locus_tag="VFB80_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HZN33311.1"
                     /translation="MQPRRILVLSLALLTTAALGQDLKPGDAVAGFGTIVDPVGDCSF
                     AREGEKLTIRIPGAYHDLWPVAGKVNAPLVLQEAEGDFSISVAVAHIDRAERDTRIAG
                     LAGTASFHAGSLVIWQDAKNFVRFDRTQMDRSGQQITSCYLHVFKDGARLVELSLVMP
                     NRPTQLRLTRKADTLTAAYSQDGGTSWKELPSQKIELPAKVKAGVSALNNTTRGNSVQ
                     FEGLKIEK"
     gene            3577..4227
                     /locus_tag="VFB80_05810"
     CDS             3577..4227
                     /locus_tag="VFB80_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33312.1"
                     /translation="MRLLILVLSFLSLAAAPAAGQEQRSPPTARQRGGTLDLQFDLHT
                     HPGGAPANPALGITTINVHLTIQAKGVRVDREGKFSLEAAGEYRLDSFAAGAVSVWND
                     EQRHDTLQGTGSIAEDRTLTLKLEWTHGPGLLIYTVNPGGTFSARQPAVAVQHVMEWK
                     LAPASRRDEPLSDGQRREITTYKARRPSTVVGTQAAAMTESVEIKHQRDVEIVPRG"
     gene            4306..4851
                     /locus_tag="VFB80_05815"
     CDS             4306..4851
                     /locus_tag="VFB80_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33313.1"
                     /translation="MALAYAAAAEAQVYRAKVNGTDRYNVVLTDDTPQQPGARLQLTP
                     LDSAYDECQRTWYLDGQITQTAGTQTGTFSGNMTRCTTKSLKDECDRQRKPIGPNYDK
                     PASGTFETKPGVGLVLTVNWQAEKWNQTDCKFEKQDRGTDVILITSVTTGDTHDPRTP
                     TIRDLIRGATYGVGGGSPMRP"
     gene            4887..6362
                     /locus_tag="VFB80_05820"
     CDS             4887..6362
                     /locus_tag="VFB80_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33314.1"
                     /translation="MTASVPMFHGLALAALWVALANLTVKAQASPADSAEVTALKAKA
                     TAGEVGAMFELAERYRLGRGLAINWDEAARWFGEATRKGHLPSTERLGEMQLFGRGLA
                     QNDLAARNCLEHAADNGDGRAMYFLGLLAAEGRGVRQDPAAALDWFRKSAAAGEARGM
                     VSLGTALASKLGGPAKEGEAEAWLAKGREVHDQSAAQGDAESAFALGVLLQFGIGAPA
                     DPAKAVDAFRRAAELGSTDAMLMLGRMFEQGEGIAKDAEQCRAWWLKSAERGDPRGMH
                     LLAVGYDEGTLGTKNPAEAAKWARLGADAGYAPAMFLLGQCYQAGAGVARDDKQAREW
                     FAKGARLRHGPSLTQVGLAAAAGSGGKGDPVLAAMLFRSAAEAGDVDGAFHLAEAYVA
                     GRGLDQDEKQALRWYREAAYLGHDAAGYQLARLYAAGQGIDKNDKLAAFWHSHSAHLG
                     HREAMLALSAAYAAGTGLEKDERLAAEWKAKAEAANKQPPK"
     gene            6369..7130
                     /locus_tag="VFB80_05825"
     CDS             6369..7130
                     /locus_tag="VFB80_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33315.1"
                     /translation="MAGQIPFRVVGPAAIWLAAVVWTAAAASGQQPGVPNPHAGEWSL
                     EFRVATRPGGETRIASSMGDDVQTVNLLAHLRRPSFTVAADGVVRWEQREGGQILWDD
                     FSTAAGKSNLSDCQALLRAEGQAIATPAPEGSGRAYDRRLTLNLAWAGSSGRGQDHRG
                     VPFAVTLSADGSKWITNYGTAAVAYEHSTWDLEQSSFQKDEISPDVIRETTVFRASRQ
                     KTTETYKFGHPHDGFPVSERIEIKHVRYPQLVPRG"
     gene            complement(7157..8392)
                     /locus_tag="VFB80_05830"
     CDS             complement(7157..8392)
                     /locus_tag="VFB80_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZN33316.1"
                     /translation="MKAHAAVQLYFDRAADVLGLDPAIRRLLVIPKREVQVQIPVQMD
                     DGRLETFVGYRVQHDDSRGPMKGGLRYHPEVDLDEVRALATLMTWKTAVVELPYGGAK
                     GGIAIDPRKLSIKELERITRKFTDQIHDLIGPHEDIPAPDMGTSWREMAWIRNQWEKY
                     HGFNPAVITGKPAEHYGIEGREEATGRGVGILAYKLLGHLNRKPQQTRVALQGFGNVG
                     SHAAKYLHESQYKIVAISDHTGGFYRKEGLDIPAMLKHAINNKGLLQGYTAADKITNE
                     QLLQLDVDLLIPAALGGVITKQNAELINAPVIIEGANAPTVPDADDILHHRGVTVLPD
                     ILANAGGVTVSYFEWVQNLQYYKWGLNRVRQELEAILSRAFDNVWQEAASHKCSLRTA
                     AYCIALRRVKAATELAGNQ"
     gene            complement(8697..9086)
                     /locus_tag="VFB80_05835"
     CDS             complement(8697..9086)
                     /locus_tag="VFB80_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZN33317.1"
                     /translation="MKHEELTGKIIEVFYEVYNELGHGFLESVYENAMRIALTQAGLA
                     VAEKQPIAVWFRGVVVGTFEPDITVQCLVILELKSGRALDSAHESQLLNYLRATEVEV
                     GLLFNFGPKPEFKRMVFDNSRKRIPKQ"
     gene            complement(9308..10741)
                     /locus_tag="VFB80_05840"
     CDS             complement(9308..10741)
                     /locus_tag="VFB80_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33318.1"
                     /translation="MKPNMDLSVANRRSFLAQAGGGFGALALAAMLAEDRAQAAGGKS
                     AEGPKRPHFDPRAKRVIWLFMHGGPSHVDLFDPKPDLIKLSGQPIPESFGMVMTRRKV
                     ATNPLLAPIKPLRPRGQSGLEVSDFLPRISEHADELCVIRSLHGDSVNHPQSVYQMNT
                     GSILMGHPSVGSWVAYGLGSENRDLPAFVVLPDPGGGLKGGPPAWGSGYLPASYQGVT
                     MRPGAQPILHLRPQPGISLAQQRATLDLVQKLNRDHLAARENDDDLSARIKAYELAFR
                     MQSAAPDLVDLKDESAETLSLYGLDQKETRDFGQRCLLARRMIERGVRFVQVYSGDTN
                     GWDAHNDVLDNHTKHCKATDQPVAALLTDLKRRGLWNETLVVWGGEFGRMPMSEQGKG
                     RDHNPWGYTVWLAGGGVKAGLAYGATDPVGLRAAENKVHIRDFHATLLHLLGLDPLAL
                     SYFHNGLDERLVGPDNKAVRVVSEILA"
     gene            complement(10825..11730)
                     /locus_tag="VFB80_05845"
     CDS             complement(10825..11730)
                     /locus_tag="VFB80_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33319.1"
                     /translation="MSVTFQLPPSGRNFEVFEAVIVGGKSTRDAAGQFRLSQSRVCQI
                     VRRMQAWQAEVLTSEQHLTDEEQLRLSKDIAGHRLNHLYCEVMGAWRRSQGDFKQTRS
                     SRYGDEVTTTKSSYGDPKYLVAASRLIKAAADLGVAGSLPYPDDEEEPGRPEVRPTAT
                     HPVEDCSQNGHARSVPAPAAMNSAAAKAGSEQTSAELSPQHALARRALLAPVQQDHAA
                     ELASAGRVGQASPRAQTHAASTLDPRGSSAGDDRSAVSPALTHPTQGGGIQLQVTPDQ
                     PGISSRPLSRQQRRKLQRALRRERA"
BASE COUNT         2185 a         3922 c         3984 g         2000 t
ORIGIN      
        1 cattcggcgc accggcggcc gcggcgatcg ccgggccgaa ttcaccggcg gcgagcttcg
       61 ccgccacctc cgcggcagga tcggccgccc aggagctcag tggaagtaac aacgcgccgc
      121 agatcgacac ccagcacgcg cgcgacatga aacacctctt ctcaaaccag cgcccctgag
      181 aaggagttgg cttcagtgtc gcagcggctg gcctcggagt caagtttgcc caggccgcgc
      241 ggtccgcttg ctccgcaagc ggaccgtcct cacgcggagc gtgaggtcta cttaccacac
      301 gccgggaatc aaccgcgagc ggacttgctg ctgataagcc tgatagtgag gatcggcgga
      361 aaggagggtt tcttcggccc atattcgcca cgcgaccccg ggcacgagca gcacccaggc
      421 gctggcagcg agccaaccag gcccggcgag aaagcaggcc agcgtcatga tcaattcgcc
      481 gaggtacgcc ggatgccgca cgagggcgta cgggccggcc tgcaccgttt gccgcagggc
      541 gggcatgacg ccgaacgatc ggcccagcca ggccagcgcg acgagcgtga gcagcgctcc
      601 ggctgcaaac aggccatggg cccaatacgg ccagcttgtc agcccaggtg caagtcggag
      661 ggccaggcca gaaccgatca tcgacggcag gcacgaagcc agttgccagg gcgcgcccag
      721 cgacaccacc ggacgccggg cgaaaaagag cacggcaacc aaaccgttga tcgccgccac
      781 gccgatcacc gccggcagcc agaactcccc cgccccggca tgccagcagc gcatgacggc
      841 cgcgatcaac gccgaaaccg caaacgcccg attagcccac gcttccgtat cgccggccca
      901 cgctggagca gaaacgtcgc ccgattcggc cgctggcgcc acgattaata gaaaaaccat
      961 ccccagttaa acaaataact gaggacgttc agcagcacga tgatgccgat gaagaccacc
     1021 aatccgccta ttcctttgcc attcatgccg atgactcccg aatgcaaggc ttgggtttct
     1081 tcgggcgaag cggcagccga tcccgcggct gcttcagccg aacgaggggc attgtgacgg
     1141 gtttttgcgg caagtcaatc tcgattgcca ctggctgggc cgccctagca cggtggcttg
     1201 ccgcccgcca aagaaattcg tgacaagcgg ccggtcctgt cgcccagttc tggtcagcgg
     1261 gttttccagg aggccgatca tgaacgctcg ggcgctattg tcgttgtggt tgatcgcagg
     1321 ggctcttgtc gcgggggcac tcggccaggc gccggcgccg gtcaagccac taggtgaagc
     1381 cgatctggcc tcgctgttgg gcctgggcct cgacgacgag acgatcgtcg cgcggatcaa
     1441 gaagggcggt ctggcgttcg acctcaccga cgccgcagtg cagaaactga agtccgcggg
     1501 cgcttcggag gcagttgtca aggcgttgca agcagccgcc caggccaaca gccagccgac
     1561 gtccgccgcc tcggcaatta cctacgacca ggtcctgcag ttgctcgggc tggggctcga
     1621 tgaagagtcc gttctcaagc ggctggccaa atcgccgacg gtcttcacgc tcgacgccgc
     1681 gcagaccgaa gcgctcaaga aggccggagc cacgcccaag atcctggagg cgctgaccgg
     1741 cgtacgggcc gcgtcagccg gcggcgacgt gacggacctg gctctgatcc tggattgctc
     1801 cggcagcatg agccaggcga cgaccggcgg cgaaaccaaa atgaccgccg ccaaacgcgt
     1861 gctgaccgac ctggtgggca agattcccgc cggcctcaac gtgacgttcg tcatctatgg
     1921 ccacgaagtc tatggcggcg ccgaggatcc acggaactgc caggccgtca aggtcgttcg
     1981 cccgctggca ccgctcgacg aggcaggccg gaccgcgctc gtgacgacga tcgccgcact
     2041 gaaaccgacc ggggcgacgc cgatcgcgct gtcgctgcgg accgcgggcg aggaattggc
     2101 caagaacaag tccatgtgcg ggctcgtgct cgtcaccgac ggcattgaat cgtgcaaggg
     2161 cgatccagcc gccgaggccg ccgcgctggt agcaaagctg aacgtcagct tcggcgtcaa
     2221 cgtcgtgggc ttcggggtga agcccgagga aaacgcggcc ttgaaggaga tcgccgtcgc
     2281 cggcaagggg aagtactacg gcgctgccga ttcaaaggag ctgaccgacg ccatttcgac
     2341 cattgccaag cagattcagg acaacgcgga gatcgtcgat acgacccgcc gcgccctccg
     2401 cgtcctgact cccaagaagg tgcagatgcc gccggtggag gagatttaca tcgtgcgcaa
     2461 cgaagaggcc gattcgccta tcgccacgaa gatcggcagc gtggagaagt tcgacgacta
     2521 tctcagcatc ccctcctcaa cgaacaagta cgccgtcttc tttcaggcaa aagacggctg
     2581 ggcggccgtc ccgctgctcc gcaatttttc cataccggat cggcgagtcg tggacgtcaa
     2641 gcccgaatcg atcctgggca tggtgcaggt gaatggcacg ggcaagaccg agcggattta
     2701 cattcgcaag cccggcgacc gcggccaggt gaaccctccg gtgcagctcg ccgaaaagtt
     2761 cggcgacgtg atggtagtgc ccgccggcaa gtacgacatc tacgtcggca acagcctgat
     2821 cgaagaagat ttcgaagtga agcccggcac gctccaccgc ctgaaatagc aacggaacga
     2881 acaatgcagc ccaggcgcat tttggttttg agtctggcac tgctgacgac cgcggcgctg
     2941 ggccaggatc tgaagcctgg cgatgcggtc gccggctttg gcacgatcgt cgatccagtc
     3001 ggcgactgct cgtttgctcg cgagggcgag aagctgacga ttcggattcc cggcgcgtac
     3061 cacgatctgt ggccggtcgc gggcaaggtg aacgcgccgc tcgtgctgca ggaggccgag
     3121 ggagacttca gcatcagcgt ggccgtggcc cacatcgacc gagccgagcg cgatacgcgg
     3181 atcgccggcc tggccggcac ggcgtcgttt cacgccggct cgctcgtgat ctggcaggac
     3241 gcgaagaact tcgtccgctt cgaccggacg cagatggacc gtagcgggca gcagattacg
     3301 tcttgctatc tgcacgtatt caaggacggc gcacgcctcg tggagctttc cctcgtgatg
     3361 cccaaccggc cgacgcaatt gcggctgacg cgcaaggcgg acacgctgac cgccgcctac
     3421 agccaggacg gcggtacgag ctggaaggaa ctgccgtcgc agaagatcga actgcccgcc
     3481 aaggtgaagg ccggagtctc cgctctcaac aacactacgc gcggcaactc agtgcagttc
     3541 gaggggctga agatcgagaa gtaatcgagt gtcgcgatgc gattgctcat cctcgttctg
     3601 tcgtttttgt cgctggcggc cgcgcccgcc gccggccagg agcagcgcag cccgccaaca
     3661 gcaagacagc gcggcggcac gctcgacttg cagttcgacc tgcacacgca tccgggcgga
     3721 gcgcccgcca acccggctct ggggatcacc acgatcaacg tgcacctgac gatccaggcc
     3781 aaaggcgtcc gcgtcgaccg cgagggaaag ttctcgctgg aggctgcggg cgagtaccgt
     3841 ttggacagct tcgccgctgg cgccgtctcc gtctggaacg acgagcagcg ccacgacacg
     3901 ctccagggta ccggctcgat cgccgaggac cgcacgctca cgctcaagct ggagtggacc
     3961 cacggccctg gcctcttgat ctatacggtc aaccccggcg gcacgttttc ggctcggcag
     4021 ccggcggtcg ccgtgcagca cgtcatggag tggaagctcg cgcccgcttc gcgcagagac
     4081 gaaccgctga gcgacggcca gcggcgcgag atcaccacgt acaaagcacg ccgcccatcg
     4141 accgtcgtcg gcacgcaggc cgcggcgatg acggagagcg tcgagatcaa acaccagcgg
     4201 gacgtcgaaa tcgtgccccg cggatgagtc tactgggcca ttgaggagac aaagtgtatg
     4261 tgtggctgca aaccaggatt caggagatgg gtcgcgggat tgctcgtggc gcttgcctat
     4321 gcggcggcag ccgaagccca ggtttatcgg gcgaaggtca atggcacaga ccgatacaat
     4381 gtagtcctga cggacgatac gccccagcag ccgggcgcca ggctccagct cacgccgctc
     4441 gacagcgcct acgacgaatg tcagcggacc tggtatctcg atggccagat cacgcaaact
     4501 gcgggcacgc aaaccggcac gtttagtggc aacatgacgc ggtgcacgac caagtccctc
     4561 aaagacgagt gcgaccggca gcgcaaaccg atcggcccga actacgacaa gccggccagc
     4621 ggcacgtttg agaccaagcc cggagtcggc ctggtcctga ccgtcaattg gcaggccgag
     4681 aaatggaatc agacggactg taagttcgag aagcaggacc gtggcacgga cgtgatcctg
     4741 atcacgagcg tgacgacggg agacacgcac gacccaagga ctccgaccat ccgcgatctg
     4801 attcgcggag cgacctatgg cgtgggagga ggcagtccga tgcgcccctg agcgtctcgg
     4861 ttctgatcgc gatgggagaa aacgtcatga ctgcatcggt gcccatgttt cacgggctgg
     4921 cactcgccgc gctctgggtg gcgctcgcga acctcacggt caaagctcaa gcatctcccg
     4981 cggattccgc ggaagtgacc gcgctcaagg ccaaggccac agcgggtgaa gtcggcgcga
     5041 tgttcgaact ggccgagcgc taccgattgg ggcgcggcct agcgatcaac tgggacgagg
     5101 ctgcccgctg gtttggtgag gcgacacgca aaggccattt gccttcgacc gagcgactgg
     5161 gcgaaatgca actgttcggc cgcggcctgg cccagaacga tctggccgcg cggaactgcc
     5221 tggagcacgc cgccgacaac ggcgacggcc gggctatgta tttcctgggc ctgctcgccg
     5281 ccgaaggccg cggcgtgcgg caggatcccg ccgccgcgct cgattggttc cgcaagtccg
     5341 cggcagccgg cgaggcgcgc ggcatggtga gcctggggac tgcgctggcg tcgaaactgg
     5401 gcggcccggc caaggagggc gaggccgaag cctggctcgc caaaggccgc gaagtccacg
     5461 atcagtccgc ggcccaaggc gatgcggaaa gcgcctttgc tctcggcgtg ttgctacagt
     5521 tcggaattgg cgcgcccgcc gatccggcca aggccgtgga cgccttccgc cgcgcggccg
     5581 agctgggctc gaccgacgcc atgctgatgc tcggccggat gttcgagcag ggcgaaggca
     5641 tcgcgaagga cgccgagcag tgccgcgcct ggtggctgaa gtcagcggag cgcggcgacc
     5701 cgcggggcat gcacctgctg gccgtcggtt acgacgaggg cacgctcggc acgaagaatc
     5761 cggccgaggc cgcgaaatgg gcgcgcctcg gggccgacgc cggctacgca ccggccatgt
     5821 tcttgctggg gcagtgctac caggcaggcg cgggagtcgc ccgggacgac aagcaggccc
     5881 gcgagtggtt tgccaaaggc gctcgcctgc ggcacggccc ttcgctgacg caggtgggcc
     5941 tggctgccgc cgccggcagt ggcggcaagg gcgacccggt gctggcggcg atgctcttcc
     6001 gcagcgccgc cgaggccggc gacgttgatg gagcgtttca cctcgccgag gcgtatgttg
     6061 ccgggcgcgg cctggaccaa gacgagaagc aggccctgcg ctggtatcgc gaagcggcct
     6121 atctggggca cgatgctgcc ggatatcagc tcgcccggct gtacgccgcc ggccagggga
     6181 tcgacaagaa cgacaagctg gctgcgttct ggcacagcca ttcggcccac ttggggcacc
     6241 gcgaagcgat gctcgccctc agcgcggctt atgcagccgg cacgggcctg gagaaagacg
     6301 aacgtctggc cgcagaatgg aaggccaagg cggaggcggc aaacaagcaa ccaccgaagt
     6361 gaacgaaaat ggccggccaa attccatttc gagtcgttgg ccccgcggcg atctggctgg
     6421 ccgcagttgt gtggacagcc gcggccgcct cgggccagca gcccggggtg cccaatccgc
     6481 acgcgggcga gtggtcgctg gagtttcgcg tcgccaccag gccgggcggc gaaacgcgga
     6541 tcgcctccag catgggcgac gatgtgcaga ccgtgaactt gctcgcgcac ctccgccggc
     6601 cgtcgttcac ggttgccgcg gacggcgtgg tgcgttggga gcaacgggaa ggcggccaga
     6661 tactctggga tgacttctcc acggccgcgg ggaaatccaa tctgtccgat tgtcaggcgc
     6721 tcctccgcgc agaaggccaa gccatcgcga cacccgcgcc ggaaggttcg gggcgcgcgt
     6781 acgaccgccg actgaccctc aacctggctt gggccggctc gagcgggcgg ggccaggacc
     6841 accggggcgt gccgttcgcg gtcaccctct ccgccgacgg cagtaagtgg atcaccaact
     6901 acggcactgc cgcggtcgca tatgaacact ccacgtggga cctggagcag tccagcttcc
     6961 agaaagatga aatctccccg gacgtgattc gcgagaccac cgtcttccgc gccagccgtc
     7021 aaaagacgac cgagacgtat aagttcggcc atccgcacga tggctttccg gtcagcgagc
     7081 ggatcgagat caagcacgtc cgctacccgc agctcgtgcc gcggggctag gcggtaggac
     7141 ggattgttaa tccggcctac tggttcccgg ctagctcggt cgcggccttc acgcggcgga
     7201 gggcgatgca gtacgcggcg gtccgcaggc tgcacttgtg gctcgccgct tcctgccaga
     7261 cgttgtcgaa ggcccggctg aggatcgcct ccagctcttg ccgcacgcgg ttcagacccc
     7321 acttgtaata ctgcaggttc tgcacccact cgaagtagct cacggtcacg ccgccggcgt
     7381 tggccagaat gtcaggcagg acggtcacgc cgcggtggtg caggatgtcg tcggcgtcgg
     7441 ggacggtcgg ggcgttggcc ccctcgatga tcaccggggc gttgatcagc tcggcgttct
     7501 gcttcgtgat cacaccgccc aaggccgccg gaatcaagag atcgacgtcc agctgcaaga
     7561 gttgttcgtt ggtgatcttg tcggcggccg tgtagccttg cagcagacct ttgttattaa
     7621 tcgcgtgctt cagcatcgca ggaatgtcaa gcccttcctt gcgatagaaa ccgcccgtgt
     7681 gatcgctgat cgcgacaatc ttgtattgcg attcgtggag gtacttggcg gcgtgcgagc
     7741 cgacattgcc gaagccttgt agcgcgacgc gagtctgttg cggcttgcga ttgaggtgcc
     7801 ccagcagctt gtaggccagg atgccgacgc cgcggccggt cgcctcttcg cgcccctcga
     7861 tgccgtagtg ctcggccggc ttgccggtga tcacggccgg attgaagccg tgatacttct
     7921 cccattggtt gcgaatccag gccatctcgc gccagctggt gcccatgtcg ggcgcgggaa
     7981 tgtcctcgtg cgggccgatc aggtcgtgaa tctggtcggt gaacttgcgg gtgatccgtt
     8041 ccagttcctt gatgctcaac ttccgcggat cgatggcgat gccccctttc gccccgccgt
     8101 acggcagctc gaccaccgcc gtcttccagg tcatcagcgt ggccagggcc cgcacctcat
     8161 ccagatcgac ctccgggtga taccgcaggc cgcccttcat cggcccccgc gaatcgtcgt
     8221 gctgcacacg gtagccgacg aaagtctcca gccgcccgtc gtccatctgc accggaatct
     8281 gcacctgcac ttcccgcttg ggaatcacca gcaggcggcg gatggccggg tccaggccca
     8341 gcacgtcggc cgcccggtcg aaatagagct ggacggctgc atgcgctttc atcggggcct
     8401 cgcgaatggg tccaagggct gcggcgtctt ggccgcacct gcaattctag cccttgctct
     8461 acggtgcaca ggccgcggca cgaggaatta ttgccgcgga taaacgcgga tgacacggat
     8521 cggcaggagg caatcctatt aacgatctat ggaagcggca gagatggtct gagttcactc
     8581 cggcggacat cgctccttca atcgcagaac gactaagcag ttggcgctgt ctttccgatc
     8641 cgtgtcatcc gtggtgatcc gcggccaatc tctgcccggc ctggcaaccg cggcgttcac
     8701 tgcttgggga tccgtttccg tgagttgtcg aacaccattc gcttaaactc cggcttaggg
     8761 ccaaagttga ataagaggcc cacttcgacc tccgtggccc gcaaatagtt cagaagttgc
     8821 gattcgtggg ctgagtccag cgcgcggccc gacttcagct cgaggatgac gaggcactgc
     8881 accgtgatgt ccggctcaaa ggtgccgacc acgacgccgc gaaaccaaac cgcgatcggt
     8941 tgtttctcgg cgacggcgag tccggcctgc gtaagtgcga tccgcatggc gttctcatag
     9001 accgactcca gaaagccgtg ccccagctcg ttgtagacct cgtagaacac ctcgatgatc
     9061 ttgccagtca actcttcgtg cttcatggcg attcctcctg gtgtgatgca gccgcagaca
     9121 ggaggaatta ttgccgcgga tgaacgcgga tgacacggat cggcagttag ataggcagag
     9181 gcaaaatccg caatccgtat tgggccctgt cgccttcaag gctccgaaag tttgttcaac
     9241 tagcgccgtc gtttccgatc cgtgtcatcc gcgttcatcc gcggccaatc tctgccctgt
     9301 tctgcggcta cgccagaatc tcgctgacca ctcgcacggc cttgttgtcg gggccgacga
     9361 ggcgttcgtc gaggccgttg tggaagtagc tgagggccag cgggtcgagg cccaagaggt
     9421 gcagcaaggt cgcgtggaag tcgcggatgt ggaccttgtt ctcggcggcc cggaggccaa
     9481 cggggtcggt cgcgccgtaa gccaggccgg ctttgacgcc gccgccggcc agccagacgg
     9541 tgtagcccca gggattgtgg tcgcggccct tgccctgctc gctcatcggc atgcggccga
     9601 actcgccgcc ccagacgacg agcgtttcgt tccacagccc ccgccgcttg aggtcggtca
     9661 gcagggcggc caccggctga tcggtggcct tgcagtgctt cgtgtggttg tcgagcacgt
     9721 cgttgtgggc gtcccagccg ttggtgtcgc cggaataaac ctgcacgaac cgcacgcccc
     9781 gctcgatcat gcggcgggcc agcaggcacc gctggccgaa gtcgcgggtc tctttctggt
     9841 ccaggccgta cagcgagagc gtctcggccg attcgtcctt gagatccacc aggtccggcg
     9901 cggccgactg cattcggaac gccagttcgt aggccttgat ccgcgccgac aggtcgtcgt
     9961 cgttttcgcg agccgccagg tggtcgcgat tgagcttctg caccaggtcg agcgtcgccc
    10021 gctgctgcgc caggctgatc cccggctgcg gccgcaggtg cagaatcggc tgcgcgcccg
    10081 gccgcatcgt gacgccttga tagctggcgg gcagatagcc gctgccccag gccggcggcc
    10141 cgcctttcaa cccgccgccg ggatcgggca gcacgacgaa cgccggcagg tcgcggttct
    10201 cgcttcccag gccataggcg acccagctgc ccacgctggg gtggcccatc aggatgctgc
    10261 cggtgttcat ctgatagacc gactgcggat gattcacgct gtcgccgtgc aggctgcgga
    10321 ttacgcacag ctcgtcggcg tgctcggaga tccgcggcag gaagtcgctc acttccaggc
    10381 ccgattgccc gcgcggccgc agcggcttga tcggcgcgag cagcggattg gtcgccacct
    10441 ttcgccgcgt catcaccatc ccaaagctct cgggaatcgg ctgtccgctg agcttgatca
    10501 gatcgggctt ggggtcgaac agatcgacgt ggctcgggcc gccgtgcatg aacagccaga
    10561 tcacgcgctt ggcccgcggg tcaaaatgcg ggcgcttggg tccctccgcc gattttcctc
    10621 ccgcggcctg cgcgcgatcc tccgccagca tcgccgccag cgccagcgcg ccgaatccac
    10681 cgccggcctg cgccaggaag gagcggcggt ttgccaccga taggtccatg ttcggcttca
    10741 tggccggccg cctggtctgg aagaaggaat cgagcccatg aattgtaaac caatcagcgc
    10801 cccggctcaa agcggccgcc cacgctacgc cctctctcgc cttagggcgc gctggagctt
    10861 gcggcgctgc tggcggctca gagggcggct ggatatgccg ggctgatcgg gggtgacttg
    10921 cagttggatg ccaccgccct gggtagggtg ggtcaaggca ggcgacaccg cggagcggtc
    10981 gtcgccggcc gaagacccac gcgggtccag cgtgctcgcc gcgtgggtct gcgctcgcgg
    11041 actcgcttga cccaccctac ccgcggaggc gagctcggcg gcgtggtcct gctgaacagg
    11101 cgccaaaagc gcgcggcggg ccagggcgtg ctgcgggctc agctcggcgg aagtctgttc
    11161 gctgcccgcc tttgcggcgg cggaattcat cgcagccggg gcgggaactg aacgcgcatg
    11221 gccattttgt gaacagtcct cgaccgggtg ggtcgccgta ggacggacct ctggccggcc
    11281 gggctcctcc tcgtcgtccg gatagggcag gctgccggca actcccaggt cggcggcggc
    11341 cttgatcagc cgcgaggcgg cgaccaggta cttcggatcg ccgtacgacg acttggtcgt
    11401 ggtcacctcg tcgccgtagc ggctgctgcg ggtctgcttg aagtcgccct gcgagcgccg
    11461 ccaggcgccc atcacttcgc agtagaggtg gttgagccgg tggcccgcga tgtccttcga
    11521 cagccgcagt tgctcctcgt cggtgaggtg ctgctcgctg gtcagcacct cggcctgcca
    11581 ggcttgcatg cggcggacga tctggcagac gcgggactgc gagaggcgga actgcccggc
    11641 cgcgtcgcgg gtcgatttcc cgcccacgat caccgcctca aacacctcaa aattccgccc
    11701 gctcggcggc agctgaaacg tgacgctcat gttggatctc cggaggaagg ttggtcggta
    11761 gtcggtgatc ggtcagtcgg taacgcaccg actcaccgag cgaccgactc acggaccgac
    11821 cgatttccgg cacgatccta cggagcgcta tggactagtc tggtccattt gagtcaaacg
    11881 gaaaaaagat ttttccagaa aagtcccgga aggggggagc taggaaaacc gctgatcacg
    11941 cggatagcag aaggaggtca atagcacccc gcccagtccg acggtgttga gcacctccca
    12001 gcggagccaa gttcgctcca tcgacgctta tgcgatgcgg tcgtcaggct taacgggcaa
    12061 tttcgcaagt tcggcattga tcgcgtctct g
//
LOCUS       DASRJB010000210         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1752621, whole genome shotgun sequence.
ACCESSION   DASRJB010000210 DASRJB010000000
VERSION     DASRJB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752621"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFB80_05850"
     CDS             complement(<1..281)
                     /locus_tag="VFB80_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZN33320.1"
                     /translation="MTPTTIAIHGAAGRMGRRLVALGSIDPELKIVAAVDSPSSPDLG
                     KDAGLLAGAVELHVPIAGALNVQPQAVIDFSVPDAAEQILQTCLSRRVPL"
     gene            complement(338..667)
                     /gene="trxA"
                     /locus_tag="VFB80_05855"
     CDS             complement(338..667)
                     /gene="trxA"
                     /locus_tag="VFB80_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN33321.1"
                     /translation="MGANVTEFTDDNFQVEVLKSDQPVLVDFWAPWCGPCRRIAPMID
                     ELAAENMGGAKIGKVNIDENQQSAINYGVEAIPTLIVFKSGQPVQRFQGIPAKARVQE
                     ALDSAKA"
     gene            910..1908
                     /locus_tag="VFB80_05860"
     CDS             910..1908
                     /locus_tag="VFB80_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HZN33322.1"
                     /translation="MEPTSLACFLVEKDVTGKIQRGVAQRPLAELPPGDVLVRVHYSS
                     LNYKDALAAQAHPGVVRKLPHVPGIDAAGTVAESSDSRFRPGDAVIVTGYELGSGQWG
                     GWSEYVRVPADWVVPLPAGLLLEEAMALGTAGFTAAQCVAAIQLNGITPAAGEIVVTG
                     ATGGVGCLAVKLLAKLGYPVVAVTGKLQLESRLKSWGASRLIGRGDVLNTTDRPLLSA
                     RWAGAVDTVGGQTLATIVRETKTNGVVSACGLVGGTDLSLTVHPFILRGVTLAGISSA
                     LLPSDRRLEIWRKLSHEWRLDGLGELTTTIGLADLEPAIQRILKGEMVGRTVVRIA"
     gene            1927..2148
                     /locus_tag="VFB80_05865"
     CDS             1927..2148
                     /locus_tag="VFB80_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33323.1"
                     /translation="MATIIGVSIVAAIFTYGVSAALWNGEFTYHFGPTIRRRSQPVEY
                     WIVVLLLFATTVLTWAMVLGQLFGFWVKR"
     gene            2294..>3088
                     /locus_tag="VFB80_05870"
     CDS             2294..>3088
                     /locus_tag="VFB80_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33324.1"
                     /translation="MSHRTKFDPAAFCSLPLVRRAYLALTAALAVFVATLAILREPFR
                     GYLAEVHIEGPSAQGLNLEDAGLWLKRAEPGAVVLTGPAPGRAARSRVRMTYHAARPN
                     AARLRLDDLATRWLYQYLPDQLQAHRRGVLAQSRGAVTAARDREDEARTRVEVLRQQQ
                     LARYFQQPAFPKPPEVTEPSPPIAAPPAEQSELRAQLEKLRLELTRLLASFTEEHPQV
                     ITLRKQIAGLERQLQLPAAERGPQILPARQAQERAAAASHFVSTDAD"
BASE COUNT          492 a         1021 c         1049 g          526 t
ORIGIN      
        1 agcggtaccc gccggctcag gcacgtctgt agaatctgtt ccgcggcgtc gggcaccgag
       61 aaatcgatga cggcctgcgg ctggacgttc agtgcgccgg cgatgggcac atgcaactcg
      121 accgcgccgg ccaagagccc cgcatctttg cccaggtcgg gcgacgacgg cgaatcgacc
      181 gccgcgacga tcttgagttc cggatcgatg ctgcccaaag caacgagccg ccgtcccatg
      241 cggccggcgg ccccgtgaat ggcaatggtg gtgggagtca tggggccagt ttgcgacaga
      301 agccccgctg ctgcaatcgg tcagtcccgt cgcaacctca ggctttggcc gagtcgagcg
      361 cttcctgcac gcgggccttg gccggaatgc cttggaaccg ctgcacgggc tggccactct
      421 tgaagacgat caacgtcggg atcgcctcga cgccgtagtt tatggccgac tgctgattct
      481 cgtcgatgtt gactttgccg atcttcgcgc cgcccatatt ctccgccgcc agctcgtcga
      541 tcatcggcgc aatccggcgg cacggtccgc accagggggc ccagaaatcg accagcaccg
      601 gctgatcgct cttgagcacc tcaacctgga aattgtcatc cgtgaattcc gtgacgttcg
      661 ctcccatgac gattctccgt ggtttcgtgc ccgatggttg ttcaggcaat tatttcgtgc
      721 gagcaagcag ggagggcgat tgtaggtggc ggcgctgcca gccgtcaatg atccctcgat
      781 tgcagccggc gacgtaagtc gttggcccgg ttgccgatag agcgacgcgg gcggacactg
      841 gccaatgcgt ttgcggggcg agaatcgccc gcaagacacc tcgatttgtc actttcgact
      901 gaggaatacg tggaaccaac ctcgctggcc tgcttcctgg tcgagaaaga tgtcaccggc
      961 aaaatccaac gcggcgtggc ccagcggcca ctcgccgagc tgcctccggg cgacgtgctg
     1021 gtccgcgtgc actactcgtc gctgaattac aaggacgctc tggcggccca ggcccatccg
     1081 ggcgtcgtcc gcaagctgcc gcacgtgccg ggcatcgatg ccgcgggcac cgtcgccgaa
     1141 agttcggatt cccgcttccg gccgggcgac gccgtgatcg tcaccggcta cgagctcggc
     1201 tcgggccagt ggggcggctg gtccgagtat gtgcgcgtcc cggccgactg ggtcgtgccg
     1261 ctgcccgcgg ggctcttgct cgaagaggcg atggcgctcg gcacggcggg tttcactgcc
     1321 gcgcagtgcg tcgcggcgat ccagctcaac ggcataacgc ccgcggccgg cgagatcgtc
     1381 gtcaccggcg cgactggcgg cgtcggctgc ctggccgtca agctgctcgc gaagctcggc
     1441 tatccggtcg tcgccgtcac gggcaagctt cagctcgagt cgcggctgaa gtcgtggggc
     1501 gcaagccgcc tgatcggccg cggcgatgtg ctgaacacga ccgaccggcc gctcttgagc
     1561 gcgcggtggg ccggcgcggt cgatacggtc ggtggccaga cgctagcgac gatcgtccgc
     1621 gagacgaaga ccaacggcgt cgtgtcggcc tgcggccttg tcggcggaac ggacttgagc
     1681 ctgaccgtcc acccgttcat tctccgcggc gtcacgctgg ccggcatcag ctcggcgctg
     1741 ttgccctccg accggcggct ggaaatctgg cggaagctca gccacgagtg gcggctggac
     1801 ggcctcggcg agctgacgac gacgatcggc ctggccgacc tggagcccgc gattcagcgg
     1861 atcttgaagg gcgaaatggt cgggcggacc gtcgtgcgca ttgcctgact gcgacagagt
     1921 cgagccatgg ccacgatcat cggcgtgtcg atcgtcgcgg cgattttcac ctacggcgtg
     1981 tcggcggcgc tgtggaacgg ggagtttacc taccacttcg gccccacgat tcgccgccgg
     2041 tcgcagcccg tcgagtattg gatcgtcgtg ctgctgcttt tcgcgacgac cgtactgaca
     2101 tgggcgatgg tcctcgggca attgtttggc ttctgggtga agcgctgagc ggcaagccgc
     2161 gccagcagcc ggctgctggc aggcaattgc ggcggaagag cgctcaagcg ggaagcgagc
     2221 cagtgacgat agcgcgaagt gatggatcgt cggctgaggc tgcgctagca ctgctggcgc
     2281 agcaacccaa gtcatgtcac accgcaccaa attcgatccg gcggcgtttt gttcgctgcc
     2341 gctagtccgc cgggcctatc tggctctgac ggccgctctg gcggtgttcg tcgccacgct
     2401 ggcgatcctc cgcgagccgt ttcgcggcta cctggccgag gtccacatcg aaggtccgtc
     2461 ggcacaaggg ctgaacctcg aagacgccgg cctgtggctg aaacgagcag agccgggcgc
     2521 ggtcgtgctc actgggccgg cgccgggccg cgcggcccgc agccgcgttc gcatgaccta
     2581 tcacgccgcg cggccgaatg cggcgaggct gcggctggac gacctggcga cgcggtggct
     2641 ctatcagtac ttgcccgatc aattgcaagc gcatcgccgc ggcgtgctgg cccagtcccg
     2701 cggcgcggtg accgcggccc gtgaccgcga agacgaagcc cgcacgcggg tggaagtgct
     2761 ccgccagcag cagctcgccc gctactttca gcaaccggcg tttcccaagc cacccgaggt
     2821 gaccgagccg agcccgccga tcgccgcgcc gcccgccgag cagagcgaac tgcgggcgca
     2881 actcgaaaag ctgcgcctgg agctgacccg actgctggcg agctttacgg aagagcatcc
     2941 gcaagtcatc acgctgcgga agcagattgc cggcctggag cggcagctgc aattgcccgc
     3001 ggcggagcgc ggtccgcaaa tcttgcccgc gcggcaggcc caggagcggg ccgcggcggc
     3061 gagtcatttt gtttccaccg acgccgat
//
LOCUS       DASRJB010000211         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1471618, whole genome shotgun sequence.
ACCESSION   DASRJB010000211 DASRJB010000000
VERSION     DASRJB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471618"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="VFB80_05875"
     CDS             complement(<1..2678)
                     /locus_tag="VFB80_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33325.1"
                     /translation="MASGTVPSVVPPPIAESLQREQVIQRQLGRTSLHVRLIDLASGL
                     AFWLVGVVVFFIAAAAFDHLIGLGTIGRVLGLVMLVAGSAWYLVAVIWPLVARSINPE
                     YAARTIEEATPSLKNSLINFLMLRSERGKMKEIVFQALEQKAASDIAGVPVESTVDRT
                     ALIRAGYVLCGVMALFAGYKILSPKDPFQTVARVLAPWAEIARPSRVQITDVQPGHAE
                     VYHGQTATISAIVSGVRGGDPVTLLVSTADGQTIDQPVEMTLAAGGLRYECVLPSDGK
                     APSGRSAGRQGEAQPFAGPSSGLQQDVTYRIVAGDAESPLYKLTVVAAPTIIVERLEY
                     QYPAYTKKPAEKVEQQGDIKGLEGTRVTIQAIANQPIKSAWIEFDPGSGAAPQLVTLA
                     AEGSRAWGTITLELQRDRQTPWHQHYQVRFRNERAQRSEQPILHKIEVLRDLAPEVQI
                     LQPQSLRVEVPENGEQLIEVRALDPDFGLSAVRLEGAVANKPPLKIDLLPPQARELPQ
                     VTVPFAFRPQEHKLAAGDELKYVAVAEDNRTNSVTGQPEPNVSRTKEYTLVVVPPAPQ
                     QNNQGQGGQNNPQQDQQPKNQEPKNQDPKNQEPKNQDPKNQNPQDQKNQKPMNQGAGA
                     QQQPMGGQQQQPMGGQQQQPMGGQQQQPMGGQGQQQNGGQGSPMNGGAGGQQGGDPQQ
                     ANPMAGGAAGNRTNQGNPSGQPAGNDAEPAGQQGDPNATAGGQQTGTGGKPAGKAHDG
                     EAIQEALKDLQRRGQQPDGSQQPQPNGGQQNPAGGQPMPNTNQQNPNGQGQTGERQPA
                     AGEQGGQPQNAGNSGNQGQNTQTTGTPAPNEAQRNAAGQEQQGKGAREGGQKAKASDR
                     LPDEQKGSNPSRQPGDANKGGTPMGPNQTQQQPKSKA"
BASE COUNT          356 a          956 c          913 g          592 t
ORIGIN      
        1 gccttcgact tcggctgctg ctgcgtctgg ttcggaccca ttggcgtgcc gcctttgttg
       61 gcgtcgccag gctgccggct gggattcgaa cccttctgct cgtcgggcag ccggtcgctg
      121 gccttcgcct tctgaccgcc ctcgcgcgcg ccttttcctt gttgctcctg gccggcggcg
      181 ttgcgctgcg cctcgttcgg ggccggtgtg cccgtggtct gcgtattctg tccctggttc
      241 cccgagttgc cggcgttctg cggctggccg ccctgctcgc ccgcggccgg ctgccgctcg
      301 ccggtctgcc cctggccatt tggattttgc tggttcgtat taggcatcgg ctggccgccg
      361 gcgggattct gctggccgcc attgggctgc ggctgctggc tgccgtccgg ctgctggccg
      421 cggcgctgca aatcctttag ggcttcctga atcgcttcgc cgtcgtgggc cttgccagcc
      481 ggcttgccgc ccgtgcccgt ttgctgaccg ccggcggtcg cgttcggatc gccctgctgt
      541 cccgcgggct ctgcgtcgtt accggcaggc tggcccgacg gattgccttg gttcgtccga
      601 ttgccggcgg cgccaccagc catgggatta gcctgctgcg ggtcgcctcc ctgctgtcca
      661 cccgctccac cattcatcgg cgaaccctgg ccaccgtttt gctgctgacc ctggccaccc
      721 atgggctgct gctgttgccc gcccatcggc tgttgctgct gaccgcccat gggctgttgt
      781 tgctggccac ccatcggctg ctgctgagca ccagcaccct ggttcatggg cttttgattc
      841 ttctgatcct ggggattctg gttctttgga tcttggttct tcggttcttg gttctttgga
      901 tcttggttct tcggttcttg gttcttcggc tgctgatctt gctgcggatt gttctggcca
      961 ccctgtccct ggttgttctg ctgcggcgcc ggcgggacga cgaccagcgt gtactccttg
     1021 gtccgcgaga cgttgggctc cggctgaccg gtgacagagt tcgtgcggtt gtcctccgcc
     1081 acggcgacgt acttcagctc gtcacccgcc gccagcttgt gctcttgcgg gcggaacgcg
     1141 aacggcaccg tgacttgggg cagctcgcgg gcctgcggcg gcaagaggtc gatcttcagc
     1201 ggcggcttgt tggcgaccgc gccctccagg cgcaccgcgg acaggccgaa gtcgggatcc
     1261 agcgcccgca cctcgatcag ctgctcaccg ttttcgggca cttcgacccg cagcgactgc
     1321 ggctgcaaga tttgcacttc gggcgcaaga tcgcggagga cctcgatctt gtgcaggatc
     1381 ggctgctcgc tccgctgggc ccgctcgttg cgaaaacgca cctgatagtg ttgatgccag
     1441 ggagtttgcc ggtcgcgctg cagctcgagc gtgatcgtgc cccaggctcg cgaaccctcg
     1501 gcggcgagcg tcaccagttg cggagccgcc ccggagcccg ggtcgaactc gatccaggcc
     1561 gacttgatcg gctgattggc gatggcctgg atggtgacgc gggttccctc caggcccttg
     1621 atgtctccct gctgctcgac tttctcggcg ggctttttcg tgtaggcggg atactggtac
     1681 tccagccgct cgacgatgat cgtcggcgcg gcgacaaccg tgagcttgta gagcggcgat
     1741 tcggcgtcgc cggcgacgat gcggtaggtg acatcttgct ggaggccgga cgaaggtccc
     1801 gcgaaaggct gcgcttcacc ctggcggccc gccgacctgc cggacggcgc cttgccgtct
     1861 gagggcaaca cacactcgta ccgcagcccg cccgcggcca gcgtcatctc gaccggctga
     1921 tcgatcgtct gcccgtccgc ggtgctcacc agcagggtga ccggatcgcc gccgcgcacg
     1981 ccgctgacga tcgccgagat ggtcgcggtc tgcccgtggt aaacctcggc gtggccgggc
     2041 tgcacatcgg tgatctgtac ccgcgacggg cgggcgattt ctgcccacgg tgcgagcacg
     2101 cgggccaccg tctgaaacgg gtccttgggc gagagaatct tgtaaccggc aaacagggcc
     2161 atcacgccgc acagcacgta gcccgcgcga atcagcgccg tccgatcgac ggtcgattcg
     2221 accggcaccc cggcaatgtc gctggccgct ttctgctcca gcgcctgaaa cacgatctcc
     2281 ttcatcttcc cgcgctcgct gcggagcatc aggaaattga tcaggctgtt cttcagcgag
     2341 ggcgttgcct cttcgatcgt gcgggcggcg tattccgggt tgatgctccg ggcgacgagc
     2401 ggccagatga cggcgaccag gtaccaggcc gagccggcga ccagcatcac cagtcccaac
     2461 actcggccga tggtccccag accgatcaaa tggtcgaatg cggccgcggc gatgaagaag
     2521 acgaccacgc ccaccagcca gaaggccagt ccgctggcca ggtcgatcag ccgcacgtgc
     2581 aggctggtgc gtccgagctg ccgctggatg acctgctcgc gctgcaggct ctccgcgatg
     2641 ggaggcggta caaccgatgg gacagttccg cttgccatat tctcctcgcg agccggcgcg
     2701 caccctgatc gcttaattat agacgacggc cggtcgggtt ttgatcccca gagacctgaa
     2761 acgcgccgca aaccgttagc acccttgcgg ttgcgactca ggcttcatcc ggctcgc
//
LOCUS       DASRJB010000212         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_343536, whole genome shotgun sequence.
ACCESSION   DASRJB010000212 DASRJB010000000
VERSION     DASRJB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343536"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFB80_05880"
     CDS             <1..754
                     /locus_tag="VFB80_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33326.1"
                     /translation="PDASQLQPELTLDRLDTRKSLLSQLNDGLPSLVASTAGRALTKH
                     QQAALSLLESPQLAAALDLRREDAATRELYGQSLFGQSCLAARRLVEAGSKVVSVFWD
                     EYGLAGSGWDTHWDHFNRMKLELSPGFDQGWYGLITDLDRRGLLNDTLVVCTSEHGRT
                     PQINKAQGGGRDHWSRVYSSLVAGGGTARGKIVGASDKQAGDVADRPISPKDLLTTMY
                     HLLGIDPHTLIQDRQGRPLPLVEGQVIGEALA"
     gene            complement(1399..2415)
                     /locus_tag="VFB80_05885"
     CDS             complement(1399..2415)
                     /locus_tag="VFB80_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33327.1"
                     /translation="MRQIRSARRGAFLALAVLVAGQSGCNALARWGSAVFVHCAESHD
                     LAQIRHDTREELSEQLIEERRQAAEREVELARYEAGRRQMESQFCLANQEALQQRLKS
                     NIRQQLESKVAFNVTQEIEVGELEVDTEKLKELMAQREQPPPVEQFQQPPPKCPCCDE
                     PCRCGSGFHRRHCPHCRHKPCEAEQKCGGPEQLQQVERQPFRQPLRPAEIPMKLPVYL
                     SFGMQQPQMERARIRQQPVLREEFREQFKQPCPCDAPVGGMPCVAPVGPPIKPPPATS
                     YPPRPPAPLPPGEIRPPVPVADPAEQARRQAGPQHRVAVRLAPASSVQPATCVEPVPP
                     GVLR"
     gene            2784..>3326
                     /locus_tag="VFB80_05890"
     CDS             2784..>3326
                     /locus_tag="VFB80_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZN33328.1"
                     /translation="MEARLPPSLSPTDATLDAPSGSSAGSGANGSDECIGEACRVHLV
                     RGSGAELSGETESLLRSRLRSAALLLFAGFASFLVWHTIRIEWNDPLRVAVYFAHAAC
                     TLVLGLIGMSLCRECRFTTRKLRWVELVTFGLPLAFFLLVQHQRIGQWTSEYGTVPDL
                     SALWLLMIFTYALFIPNRWQR"
BASE COUNT          555 a         1066 c         1105 g          600 t
ORIGIN      
        1 gcccgatgcg tcgcagctgc agcccgagct gacgctcgac cggctcgaca cgcgcaagag
       61 cctgctctcg cagctcaacg atggtctgcc gagcttggtc gcttccacgg ccggccgcgc
      121 gctcaccaag catcagcagg ccgcgctatc gctgctcgaa tcgccgcagc tggccgcggc
      181 gctcgacctg cgaagggaag acgccgccac gcgcgagctc tacggccagt cgctgttcgg
      241 ccagtcctgc ctcgccgccc gccgcctggt ggaggcgggc agcaaagtcg tcagcgtctt
      301 ctgggacgag tatggcctgg ccggcagcgg ctgggacacg cactgggacc acttcaaccg
      361 gatgaagctg gagctctcgc ccggcttcga ccagggctgg tacggcctga tcaccgacct
      421 cgaccgccgc ggacttttga acgacacgct cgtcgtctgc accagcgagc acggccgcac
      481 gccgcagatc aacaaggccc agggaggcgg ccgcgatcac tggtcgcggg tctattcgtc
      541 gctcgtcgcc ggcggcggca cggcccgcgg caagatcgtc ggcgcatccg acaagcaggc
      601 cggcgacgtg gccgaccggc ccatcagccc caaggatctg ctgaccacga tgtaccacct
      661 gctcggcatc gacccgcata cgctcatcca agaccgccag ggccggccgc tgccgctcgt
      721 cgaaggccag gtcatcggcg aagcactggc gtgacggcga tcgctgattg gaggtccgta
      781 ggtccctgag gccggcggcg gtgccaatct gaacattggt acgctatttg cagtatagtt
      841 ccctttggcg cggcggactg ccgttttggc cgcccgccgc caatgtcacc cgttctatgg
      901 caaattggtt gttggcaatc tgtggcagaa tcgccgccgt tccgcttgga ccccaaatat
      961 aacgaaaact ccccggcggg ctctctaagg cttttaaacg ctcaactgca aaaccgccgg
     1021 aggtacgccg gaagtccttg caagcctgac agttgcggcg cgacggcgat aaacgaggca
     1081 cgtgcgcgag gctgtgctgt tggcagtttt cattatattc gccgctggcc cttaagggcg
     1141 ggcgacgggc aacctggtcc gcctccaagc gacggatcgt ctctctgcga cgggctgacg
     1201 cagtctcgtt ttgagccaaa atgggtagcc tggcgacaac actttgcaat tgcattttgc
     1261 accttcgcag tttgcactct gcgatcttcc cgctggggat cgcacaagtc gcaacaccag
     1321 agaattgcaa aatgggaaat gcaaatgcaa attgcaaaaa gggctgcggg cctgcacaga
     1381 ttttgacgag tcgcactact accgcagcac gcctggcggg accggttcga cgcacgtcgc
     1441 cggctgaacg ctgctggcag gtgccagccg cacggcgacc cggtgctgcg ggccggcctg
     1501 ccggcgggcc tgctcggccg gatcggccac aggcacgggc gggcgaattt cgccaggcgg
     1561 gagtggcgcc ggcggacgtg gagggtagct ggtcgccggc ggcggcttga ttggcggacc
     1621 gaccggcgcg acgcacggca tgccgccgac cggagcatcg cacggacagg gttgcttgaa
     1681 ctgctcccgg aattcctccc gcaacacggg ctgctggcga atgcgggccc gttccatctg
     1741 gggctgctgc atgccgaagc tgagatagac cggcagcttc atcggaatct ccgccggccg
     1801 cagcggctgg cggaagggct gccgctcgac ctgttgcaac tgctcgggac cgccgcactt
     1861 ttgctccgcc tcgcagggct tgtgccggca gtgcgggcag tgccggcggt ggaagcccga
     1921 accgcagcga caaggctcgt cgcagcacgg gcacttgggc ggcggctgct gaaactgctc
     1981 gacgggcggc ggctgctcgc gctgggccat cagctccttg agcttctcgg tatcgacttc
     2041 cagctcgccg acttcgatct cctgcgtcac attgaatgcc accttcgatt cgagctgctg
     2101 gcgaatgttg ctcttcagcc gctgctgaag tgcctcttgg ttggcgaggc agaactggct
     2161 ttccatctgc cgccgcccgg cttcgtagcg ggccaactcg acctcgcgct ccgcggcctg
     2221 gcggcgctcc tcgatgagct gctcggagag ttcctcccgc gtatcgtggc ggatttgggc
     2281 caggtcgtgc gactcggcgc aatgcacgaa caccgcgctg ccccagcggg cgagcgcatt
     2341 gcagccggac tggcccgcga ccagcacggc cagcgccaag aacgcgccgc ggcgggcgga
     2401 tcggatctga cgcatggtga gcatttgcgc gaagccgcaa ccaatgagtg ttctgccggg
     2461 tagtgcacgt gtagggatta atcgagttcg cgacgcaaac gccttgattc tgctgttggg
     2521 gggtatggtg gtagcggtcg cgcgggttgt gtcacaccac tgccagcatc gcgtctgggg
     2581 tcagaaggag ttgacgcaca atgacttgca agcagtccgg cccacgcgac acctggtaga
     2641 agcaaaactt ctagtttcct ataggcccca tagccggtga cgattcggca agattcgacc
     2701 gtgctggccg aaccggatat gatggtcaga cgtctgacct gaggcccgct gccggaggcg
     2761 gcgggccgaa cgaggtgcga gccttggaag cgcgtctgcc gccatcgttg agcccgaccg
     2821 acgcgacgct cgatgcgccc tcgggaagta gcgcgggcag cggtgcgaac ggcagcgatg
     2881 agtgcatcgg cgaagcctgc cgcgtgcacc tggtgagggg cagcggggcg gaactctcgg
     2941 gcgaaaccga aagcctgctg cggtcgcggc tgcggagcgc ggcgctcttg ctgtttgccg
     3001 gttttgcgtc gtttctggtt tggcatacga tccgcatcga atggaacgac ccgctgcgcg
     3061 tcgccgttta tttcgcgcac gcggcgtgca cgctggtgct ggggctgatc ggcatgtcgc
     3121 tatgccgcga gtgccggttc acgacccgca agctccgctg ggtcgagctg gtgacatttg
     3181 gcttgccgct ggcgtttttc cttttggttc agcatcaaag gatcgggcag tggaccagcg
     3241 agtatggcac cgtgcccgac ctgagcgccc tgtggctgct catgatcttc acctacgccc
     3301 tgttcatccc gaataggtgg cagcgg
//
LOCUS       DASRJB010000213         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_344645, whole genome shotgun sequence.
ACCESSION   DASRJB010000213 DASRJB010000000
VERSION     DASRJB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344645"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFB80_05895"
     CDS             <1..685
                     /locus_tag="VFB80_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HZN33329.1"
                     /translation="HQKSFWFDHGNVNGVDFWAETAKSQGKQVQTELVKAEGGKVGTL
                     VTRNEWLGPDGGKICTDLRTLHFGGDKDARWIDFDVVVTAAADEVKFGDTKEGSFGLR
                     IAESMRVDRKTGGKIITSEGLEDDKAWGKPAPWVDYYGPVQGEQLGIAILNHPSSFRY
                     PTTWHVRTYGLFAANPWGLADFTGGKERSDHTMKKGESFTLRYRVIFHKGDEKAGKIK
                     EAFAAYSKE"
     gene            complement(753..1088)
                     /locus_tag="VFB80_05900"
     CDS             complement(753..1088)
                     /locus_tag="VFB80_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33330.1"
                     /translation="MRLVLRDWKSLVAGALTTAGLPLLVLGLTQGWSWCAVAAALVST
                     ACVGWAVWRIRLILRLLVSGPRLSGRVVHRRDVGHALVTYAFGDRVYESTIAVQGGPA
                     HFDRGAATT"
     gene            1252..1890
                     /locus_tag="VFB80_05905"
     CDS             1252..1890
                     /locus_tag="VFB80_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33331.1"
                     /translation="MDSPRPTLGALAAGAGHFAAASSPSPPAEIAEGQHALVVSAQRG
                     DHRAFAALVEMYQNTVYGFLRARVVEPADAEDMCQEVFLRCYLGREKLSRATTVGAWL
                     VGIARNILREHVRRQHRRREVAWTELCLELDALVSDPGGANDDALAHLPTCLALLGQS
                     AREAIDLRYRAQLRMAAIAERLKRSEGAIKLLVHRARAALKHCLDQKLKKPA"
     gene            1887..>2181
                     /locus_tag="VFB80_05910"
     CDS             1887..>2181
                     /locus_tag="VFB80_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33332.1"
                     /translation="MTDAELIALLEQKTPDELSLDEIDLLRRRLAESEELRQTLLGQL
                     QMETYLAEALSRVDLSPDKIVARANQQQPASSGSTLLIALLIGLPLLGLAAVIL"
BASE COUNT          413 a          723 c          720 g          325 t
ORIGIN      
        1 gcaccagaag tcgttctggt tcgaccacgg caacgtcaac ggcgtcgatt tctgggccga
       61 gaccgcaaag tcgcagggca agcaggtgca gaccgagctc gtcaaagccg agggaggcaa
      121 ggtcggcaca ctggtcacca ggaacgagtg gctcggcccc gacggcggca agatttgcac
      181 cgacctgcgg acgctgcatt ttggcggcga caaagacgcg cggtggatcg acttcgacgt
      241 ggtcgtaacg gctgcggccg acgaagtgaa gttcggcgat acgaaggagg gctcgttcgg
      301 cctgcggatc gccgagtcga tgcgggtcga tcgcaagacg ggcggcaaga tcatcaccag
      361 cgagggcctg gaagacgaca aagcgtgggg caagcccgcg ccctgggtcg attactacgg
      421 tccggttcag ggggaacagc tgggcatcgc gatcctcaat cacccgagca gcttccgcta
      481 tccgacgacc tggcacgtgc gaacgtatgg gctgtttgcc gccaacccgt ggggcctcgc
      541 cgacttcacc ggcggcaagg agcgcagcga ccacacgatg aaaaaaggcg agtcgttcac
      601 gctccgctac cgcgtcatct tccacaaggg cgacgagaag gcgggcaaga tcaaggaagc
      661 cttcgcggcg tattccaagg agtagatcgg ctatcgggac agcgtttcca ggaaaaacgc
      721 ccgcgtcggc ttgtgcggat cgatgatgat cgctaagtcg tcgccgcgcc gcgatcgaag
      781 tgagccggtc cgccctgaac ggcgatcgtc gattcataca cccgatcgcc gaacgcatac
      841 gtcaccagcg cgtgtccgac atcgcggcgg tgaaccacgc ggcccgacag gcgcggaccg
      901 gaaacgagca gccgcaagat cagccggatc cgccagacgg cccatccgac gcaagcggtg
      961 ctaacgagcg ctgcggcgac ggcgcaccac gaccagccct gcgtcagacc gagcaccagc
     1021 aagggcagcc cggctgtcgt cagcgcgccg gccacgagcg atttccaatc gcggagcacg
     1081 agtcgcattg gaaagcgcga aatcacggaa cacccctagc atttcgcagc gtcaagggcg
     1141 ggccgatgtt actcaatgcc aggacagcgg cgggaagttt gccctccggc ggcccgctcg
     1201 gctaagatga cgattgccca ccttacgatc ccgcctcctt cccgccggct catggattct
     1261 ccgcgtccca cgcttggagc gctcgctgcc ggcgcggggc attttgccgc ggccagttcc
     1321 ccctcgccgc cggcggagat cgccgaggga cagcacgcgc tggttgtctc ggcccagcgg
     1381 ggcgaccacc gcgcgttcgc cgccctggtc gagatgtatc aaaacaccgt gtatggcttc
     1441 ctgcgggcgc gggtcgtcga gccggcggac gccgaggaca tgtgccagga ggtattcctg
     1501 cggtgctatt tgggccgcga gaagctcagc cgggcgacga cggtcggggc gtggctggtg
     1561 ggcatcgccc gcaatatcct ccgcgagcac gtccgccggc agcaccgccg ccgcgaagtg
     1621 gcctggaccg aattatgctt ggagttggac gcgctggtca gcgatccggg cggggccaat
     1681 gacgacgcgc tggcccacct gccaacctgc ctggcgttgc ttggccaatc ggcccgcgag
     1741 gcgatcgacc tccgctaccg ggcgcagctc cgcatggcgg cgatcgccga gcggctgaag
     1801 cgcagcgaag gggccatcaa gctgctcgtt caccgggccc gcgccgccct caagcactgc
     1861 ctcgaccaga agctgaagaa gcccgcatga ccgacgccga actgatcgcg ctcctcgagc
     1921 agaagacgcc agacgagctg tcgctggacg aaatcgacct cttgcgccgc cggctcgccg
     1981 agtcggaaga gctccgccag acgctgctcg gccaactgca gatggagacc tacctggccg
     2041 aggccctcag ccgcgtggac ctctcgccgg acaagatcgt cgcccgggcg aatcagcagc
     2101 aacctgcctc gtcgggctcg acactgctga ttgccttgct catcggcttg ccactcttag
     2161 gattggcggc ggtcatcctg g
//
LOCUS       DASRJB010000214         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2599974, whole genome shotgun sequence.
ACCESSION   DASRJB010000214 DASRJB010000000
VERSION     DASRJB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599974"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFB80_05915"
     CDS             complement(<1..479)
                     /locus_tag="VFB80_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33333.1"
                     /translation="MRMNATLAQCALLALITTLPAADADPLKPPAIETQDVPVIPAEF
                     AARLAQYQNTRSASFAGWSPDGRGILVGTRFGNAPQLHRVYEPGGRREQVTFQSEPVF
                     GGFIPKAADGAILFSLSKGGSENNQLYLLDRAKFTTTLLTDGKSRNGLQAMRPDGKL"
     gene            complement(470..1849)
                     /locus_tag="VFB80_05920"
     CDS             complement(470..1849)
                     /locus_tag="VFB80_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN33334.1"
                     /translation="MMTELPSIAKAAREMREGALRPSDLVEHCLARIEQLEPRVKAWV
                     LVDADGARREARRQDQLLRDDEPLGPLHGIPIAIKDIIDVAGWPTLCGSPLRRGHAAV
                     KDAPVVAALRKAGAILLGKTVTTEWAGFDPPVTRNPWNLDHTPGGSSSGSAAAVATEM
                     CLAALGSQTGGSIIRPAAFCGVAGLKPGYGELPLEGIAPLAMHLDHVGPIAKRASDLY
                     IVWQALAAEAHGSMESMFSRRWADSDLETWIDAYGLNKTLFVLEGPLLDRLPADARQA
                     FEQTLNRLKSSVNLRPLRLPDSFADVLQLHRRIMAAEAAAHHRATFPSRRSEYSPRIT
                     ALLDEGLALSAAEYAEALIRREDLRAELAQLLNANEDVLGCLVMPSTVGPAPLADTTG
                     DPAFNSPWSYLGWPALTIPCGRAANGLPLGLQFVALTVPQVFAMGGLCERILGFHDRP
                     PIVEELTCE"
     gene            1983..2363
                     /locus_tag="VFB80_05925"
     CDS             1983..2363
                     /locus_tag="VFB80_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZN33335.1"
                     /translation="MTENELSHAIIAAAIEVHRTLGGPGLLEDMYEEALRFELEQRGF
                     IVRRQIGFKVIYKGRELDKRLVIDLIVNDLVIVEAKAVTDYHPIFETQLLTYLRQTNK
                     RLGLVINFGEGLVKDGIHRVVNKL"
BASE COUNT          437 a          759 c          813 g          416 t
ORIGIN      
        1 atcagcttgc cgtcgggccg catggcctgg aggccgttgc ggctcttgcc gtcggtcagc
       61 agggtggtcg tgaacttcgc gcggtcgagc aagtagagct ggttgttctc gctgccgcct
      121 ttgctgagcg agaacaggat cgccccatcg gccgccttgg gaatgaagcc gccaaagacc
      181 ggctcgctct ggaacgtgac ctgttcgcgg cggccgccgg gctcatagac gcggtgcagc
      241 tggggcgcgt tcccaaaacg cgtgccgacg aggatccccc gtccgtccgg cgaccagccc
      301 gcgaagctcg ccgagcgcgt gttctggtac tgggccagcc gtgcggcaaa ctccgcggga
      361 atcaccggca cgtcctgcgt ctcgatcgcc ggcggcttga gcggatcggc atccgcggcc
      421 ggcagcgtgg tgatcagcgc cagcaaagcg cattgcgcca atgtcgcgtt cattcgcatg
      481 tcaattcctc cacgatcggc ggtcggtcat gaaatccgag aatgcgttcg cacaggccgc
      541 ccatggcaaa cacttgcggc acggtgaggg cgacgaattg caggccgagg ggcagaccgt
      601 tcgcagcgcg gccgcaggga atcgtcagcg cgggccagcc gaggtaactc cagggcgaat
      661 tgaacgccgg atcgccggtc gtgtcggcca gcggcgcggg gccgacggtc gaaggcataa
      721 ccaggcagcc cagcacgtcc tcattggcat tcagcagctg agccagctcc gctcgcaagt
      781 cctcgcggcg gatcagcgcc tcggcgtatt cggcagccga aagcgcaagc ccctcgtcga
      841 gcagcgcggt gatccgcggg ctgtattcgc tccggcggct ggggaacgtc gcccggtgat
      901 gcgcggcggc ttcggcggcc atgatccgcc ggtggagctg cagcacatcg gcgaacgaat
      961 ccggcaggcg cagcggccgc aggttgaccg acgacttcag ccgattgagc gtctgttcga
     1021 acgcctgccg cgcatcggca ggcaggcgat cgagcagcgg gccctcgaga acgaacagcg
     1081 tcttgttgag gccgtaggcg tcgatccagg tttccaggtc actgtcggcc cagcggcggg
     1141 agaacatact ctccatgctg ccgtgcgcct cggcggcgag cgcctgccag acgatgtaca
     1201 ggtcgctggc gcgcttggcg atcggcccga cgtggtccag gtgcatggcc aagggcgcga
     1261 tgccctccag cggcagttcg ccatagcccg gcttgaggcc cgccacgccg cagaacgctg
     1321 ccgggcgaat gatcgagccg ccggtttgcg agccgagagc ggcgagacac atctcggtgg
     1381 cgaccgccgc ggcgctgccg ctggaagaac cgcccggtgt gtggtcgagg ttccacggat
     1441 tgcgagttac cggcggatcg aagccggccc actcggtggt gacggtcttg ccgagcagga
     1501 tcgctcccgc cttccgcagc gccgcgacga ccggcgcgtc cttcaccgcc gcatggcccc
     1561 ggcggagcgg cgagccgcag agtgtcggcc agccggcgac atcgatgatg tccttgatcg
     1621 cgatcggtat gccatgcagc gggccgagcg gctcgtcgtc gcgcaagagc tgatcctgcc
     1681 gcctggcctc gcgccgcgct ccatcggcat cgacgagcac ccaggccttc acccgcggct
     1741 ccagttgctc gatccgcgcc aggcaatgct ccaccaggtc gctcggccgc agcgccccct
     1801 cgcgcatctc gcgggccgcc ttggcgatgc tcggcagttc ggtcatcatg cggcctaagt
     1861 taccggccag ggccgggcga agaaatgggt gggttggcgg ggctaacgtt gttcgattta
     1921 acgcaaagga acgaaggggc aaaggccgca aagagaaaca tcaagacgag ggagcatttg
     1981 atatgaccga gaatgaactg agccatgcga taattgccgc ggcgattgaa gtgcatcgga
     2041 cgttgggagg tccgggacta ctcgaagata tgtatgagga agcgttgagg tttgagcttg
     2101 agcagcgagg tttcatcgtt cgcaggcaaa tcggattcaa agtcatatac aagggtcgcg
     2161 agctggataa gcggctggtg attgacttga tcgtcaacga tctggtgatc gtcgaggcca
     2221 aggcagtgac agattatcat ccgatattcg agacccaact cctcacgtat ctgcgtcaaa
     2281 cgaacaaacg cctcgggttg gtcatcaatt tcggagaagg gctcgtgaaa gacggaatcc
     2341 atcgagtagt caataaactc tgaaacgagc tgcttcctcg ccctcgctgc tctctctgcg
     2401 ccttttgcac ctttgcccct ttgcg
//
LOCUS       DASRJB010000215         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3728974, whole genome shotgun sequence.
ACCESSION   DASRJB010000215 DASRJB010000000
VERSION     DASRJB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3728974"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFB80_05930"
     CDS             <1..265
                     /locus_tag="VFB80_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33336.1"
                     /translation="QADHCRTARKQLATLEANRMPVFLTFPLLFCFAPAVLIILMSPA
                     FLQIGEFLNPNNPNNPLRNNETISTSSLANTINSLDQSSGTTP"
     gene            complement(397..1074)
                     /locus_tag="VFB80_05935"
     CDS             complement(397..1074)
                     /locus_tag="VFB80_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33337.1"
                     /translation="MSTTITVRRPRRKRPPSARDQALASAYLLQGKSQAELAAEWGLT
                     QARVSQIVRRVRAWRSQLSTQEQRAHQELYETVYRTAMRKLLHEEQGRTVTTVKTGPR
                     GIETTVRELPPTVKWLKVAQRAAVQLSQPPDGEAPIEPDPQQRQRIMVQELVKLRDQA
                     ERAGRLPARGRSFPLVVSLLRALQGLAPYRGEDDQGLVELGQRLLGIPPCESPDVQPP
                     RAASEGR"
     gene            complement(1414..2796)
                     /gene="mgtE"
                     /locus_tag="VFB80_05940"
     CDS             complement(1414..2796)
                     /gene="mgtE"
                     /locus_tag="VFB80_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN33338.1"
                     /translation="MIGHLMRPELEELIREQQWDVLRDALSHFHPSDIAEILVDIPEH
                     VDVPVFRVLPRKLAGQVFSHLPHHHQEALVRSVSNEQMRALLTQMTPDDQARVMDELP
                     PQVTRRILDTLSPEELKAARDLLGYPPHAAGRFMTPKYVAIRSSMTVREALDHVRKTG
                     RDKETVNVLYVLDDEGHLSEDIRLGSLVMADPDAKVGDVHEGSTVSVLATTDREEALR
                     TFEKYDRIALPVVDSERHMLGIITIDDFLDVAQKEATEDIQKIGGSEALDGPYLDVTF
                     RDMIRKRGGWLSILFLGEMLTATAMGFFEKEIEKAVVLALFVPLIISSGGNSGSQAAT
                     IIVRSLALNEVQLRDWLQVLWRELRTSLTLGAWLGLLGFIRIVAWHWLGWVDYTAHFV
                     LVGAAVFATLIGVVCFGSITGSMLPFLLRRLGFDPATSSAPFVATLVDVTGLVIYFSV
                     AYVILRGTLL"
     gene            2917..5286
                     /locus_tag="VFB80_05945"
     CDS             2917..5286
                     /locus_tag="VFB80_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN33339.1"
                     /translation="MTPVFFRPATLSAIAVIAALFCEPATAQDAPAAVEKQAAAVLAR
                     NCLECHNGSDKKGQLDLTRRERALAGGESGPALDPGHPDKSLLLKRIAAGEMPEDRPA
                     VSAAEQAVLRQWIAAGAKWDADPLDPFQFTTSRRAGYDWWSLQPIVRPTPPAIPNPKS
                     PIRNSIDQFVLQKLRAAQLEPSPQADRRTLLRRVYIDLIGLPPSPEEMEEFLSACSPS
                     PSLPLSPSAATERESGRGGERDSKAYEAVVDKLLDSPHYGERWARHWLDIVRYGESQG
                     FERNKLRPNLWKYRDWVVEAFNSDLPYDDFIRWQLAGDVLRPDDPLAVVGSGFLVLGP
                     YDLTAYNNGTPDMRAFAREEELEGLVACVCQTFLGLTVNCSRCHDHKFDPIRQTEFYQ
                     ISAAIGGTYQADERECRSASGKSAAEERIAKLREEIAALDGKPDLAAQRSRLESIIRL
                     LEGGPAHVTAPQQPKPWRVLARGDFRQPGEVVAPRGIAAVPGVSADWRLPADAPEAER
                     RKALAEWIAGRGNPLTPRVIVNRLWGHHFGAGLVPTPSDFGFQGGRPSHPDLLDWLAS
                     ELVQPSDGKPWSLKRIQRLIVLSATYRQASRFNEQAAAVDAENRLLWRQSPQRLSAET
                     FRDALLAVSGELDRKLGGPGYRDFTISSAGNNETYTVFDAVGPEHNRRSLYRTWVRTG
                     TSPLLDLFDCPDPSVPTPRRSVTSTPLQALAQLNDAAIEHYAQKFAERLRREHPGHEA
                     TQIRRAYELAFARQPSADEAAFGKQFAADHGLAQLCLVLFNANEFLYVD"
     gene            5382..>6218
                     /locus_tag="VFB80_05950"
     CDS             5382..>6218
                     /locus_tag="VFB80_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33340.1"
                     /translation="MTILSPFDVPAAPGPSRRDLLGQFATGLGGMALLALLAREGAAA
                     AVKGEADDPPPHHPPKAKRAIQIFLQGGLSQVDSFDPKPELERLHGKSVPGDEKPVAF
                     MGKVGLLHKAHFAFAQRGQSGLWVSDLFPQIATVADELTVIRSMWSGTGNHTPATYEA
                     NSGFRTLGFPAAGTWISYGLGCEVDNLPAFVVLPDSRSLPTGGSNNWSAGFLPGRHQG
                     VMFRTSGPAVRDLRPASGVSEATQAARYAAIAELNRRHLAERGRDDALESRLRSYELA
                     ARM"
BASE COUNT         1055 a         2095 c         2036 g         1032 t
ORIGIN      
        1 ccaggccgac cactgccgca cggcccgcaa gcagcttgcc acgctggaag ccaaccgcat
       61 gccggtgttc ctgaccttcc cgctgctgtt ctgcttcgcc ccggccgtgc tcatcatcct
      121 catgtcgccg gcgttcctgc agatcggcga gttcctcaac ccgaacaatc ccaataaccc
      181 gctgcggaac aacgagacca tcagcaccag cagcctggcc aatacgatca acagcctgga
      241 ccagagttcg gggaccactc cataagaccc ccggttgact gagtcgaaac tgcttctgtt
      301 ttccagtttt ccaccttaac tcctggcctg ccatgggttt gcggcgactc gtcccgcagg
      361 ccgataagta ctactttatt agcgtttgag ggcttgctag cggccttctg aagcggcacg
      421 cggcggctgc acatcggggc tctcgcacgg cggtatcccg agcagccgct ggcccagctc
      481 gaccagcccc tggtcgtctt ccccgcggta aggcgcgagg ccctgcaggg cccgcagcag
      541 cgatacgacc agcggaaagg agcggcctcg cgccgggagg cggccggccc gctcggcctg
      601 gtcgcggagc ttgacaagct cttgcaccat gatccgctgc cgctgctggg gatcgggctc
      661 tattggcgct tccccgtctg gcggctggct gagctgcact gcggctcgct gggcgacttt
      721 cagccacttg acggtgggtg gcagctcgcg gacggtcgtc tcgatgcccc gcggtccggt
      781 cttgaccgtg gtgacggtgc ggccttgctc ctcatgcagc agcttccgca tggccgtgcg
      841 atagaccgtc tcgtacagct cctggtgggc ccgctgctcc tgcgtgctga gctggctccg
      901 ccaggcccgg acgcggcgga cgatctgcga cacgcgggct tgcgttaggc cccactccgc
      961 ggccaactcc gcctgggatt tgccctgaag caggtaggcg gaagccagcg cctgatcgcg
     1021 ggcgctgggc gggcgcttcc gccgcggacg gcggaccgtg attgtcgtgg acacaggatt
     1081 ctcctttcag cgaagccgct gcggcccggc gggccgcgag tggaccatcc acccgcggcg
     1141 aaccggcagc ccagaggctt tgccgcagtt tcgcaacccg atatgagtct gaaaagcaac
     1201 ttgtgtacat tttgttcaaa cgtcctttcg cgcatgtcat agcaagagta gatagagtac
     1261 agacttacat aaacgccgct cgccgtaagt cctgaaaaaa aactttttcc gcgcgctggc
     1321 gaaatggact agtgtggtcc attctggacg gccctctttc ggggtacgcc ggacgattcc
     1381 cgcgcggcaa accggggcga accactaagc ggcctaaagc aaagtcccgc ggaggatcac
     1441 gtaggcgacc gagaagtaga tgaccagccc cgtgacatcg acgagcgtgg cgacgaatgg
     1501 agccgagctg gtggcgggat cgaagcccag gcggcgcagc aagaacggca gcatgctgcc
     1561 ggtgattgaa ccaaagcaga cgacgccgat cagcgtggcg aagaccgccg cgccgacgag
     1621 cacgaaatgc gccgtgtaat cgacccagcc cagccagtgc caggcgacga tgcgaatgaa
     1681 gccgagcagc cccagccacg cgcccagcgt gaggctggtt cgcagctcgc gccacagcac
     1741 ctgcagccag tctcgcaact gcacctcgtt gagcgcgagc gaccggacga tgatcgtggc
     1801 ggcctgggag ccggagttgc cgccgctgct gatgatgagc ggcacgaaga gggccaggac
     1861 caccgccttt tcaatctcct tctcgaagaa gcccatcgcg gtggcggtta gcatttcgcc
     1921 gagaaacagg atcgagagcc agccgccgcg cttgcggatc atgtcgcgaa acgtcacgtc
     1981 gaggtaaggg ccgtccaggg cttcggagcc gccgattttc tggatgtcct cggtcgcttc
     2041 cttctgggcg acgtcgagga agtcgtcgat cgtgatgatg cccagcatat gccgctcgct
     2101 gtccaccacc gggagcgcga tgcggtcgta cttctcgaaa gtccgcaggg cctcctcgcg
     2161 gtcggtggtc gcgagtaccg acacggttga gccttcgtga acgtcgccga ccttggcgtc
     2221 gggatcggcc atgaccagcg agccgagccg gatgtcctcg ctcaggtgtc cctcgtcgtc
     2281 caggacgtac agcacgttga cggtctcttt gtcgcggccg gtcttgcgga cgtggtccaa
     2341 ggcctcgcgg acggtcatgc tgctgcggat cgcgacgtac ttgggcgtca tgaagcgtcc
     2401 ggccgcgtgc ggcggatagc ccagcaggtc gcgggccgct ttcagctcct ccggagagag
     2461 cgtatcgaga atccgccgcg tgacctgcgg cggcaactcg tccatcacgc gggcctggtc
     2521 gtcgggcgtc atctgcgtca ggagcgcccg catctgttcg ttcgataccg agcggaccag
     2581 ggcttcctga tggtggtgcg gcaggtgcga aaaaacctgc cccgccagct tccgcggcaa
     2641 cacacgaaaa actggcacat cgacgtgctc gggaatatcg accaggattt ccgcgatgtc
     2701 cgagggatgg aagtgcgaca gggcgtcccg cagcacgtcc cattgctgct cgcggatcag
     2761 ctcctccaac tccggccgca tcaggtgacc gatcatggcg taatgctccg gctgcgatga
     2821 agctgtcaag gatagcaccc gcattcgtgc ggcaagcggg cgggaagatt acaattggaa
     2881 ccgaccctcc cgccgtctga ctccccgcca gttcccatga cgccagtctt cttccgaccg
     2941 gccacgctga gcgcgatcgc tgtcattgcg gcgctgttct gtgaaccagc gaccgctcag
     3001 gacgcacccg ctgcggtcga gaagcaggcg gccgcggtgc tggcccgcaa ctgcctggaa
     3061 tgccacaacg gctcggacaa gaagggccag ctcgacctga cccgccgcga gcgggcgctg
     3121 gccggcggcg aaagcggccc ggcgctcgat ccgggccatc ccgacaagag cctgctcctc
     3181 aagcggatcg cggcggggga aatgccggaa gaccgcccag ccgtgtccgc cgcggaacag
     3241 gccgtcttgc ggcagtggat tgccgccggc gccaaatggg acgccgatcc gctcgacccg
     3301 ttccagttca ccaccagccg ccgggccggc tacgactggt ggtcgctgca gccaatcgtt
     3361 cgccccacgc cgccagcaat cccaaatccc aaatccccaa tccgcaattc aatcgaccag
     3421 tttgtcctcc aaaagctccg cgccgcgcag ctcgaacctt cgccgcaggc cgaccgccgc
     3481 acgctcttgc ggcgcgtgta catcgacctg atcggcctgc cgccgtcgcc ggaggagatg
     3541 gaggagtttc tcagtgcctg ctccccctct ccctctctcc ccctctcccc ctctgccgcc
     3601 acagagaggg agagtgggag agggggagag agggacagca aagcctacga ggcggtcgtc
     3661 gataagctcc tcgactcgcc gcattacggc gagcgctggg cgcggcactg gctcgatatc
     3721 gtgcggtacg gcgagagcca gggtttcgag cggaataagc tgcggcccaa tttgtggaag
     3781 tatcgcgatt gggtcgtcga ggcgttcaat tccgacctgc cctacgacga cttcatccgc
     3841 tggcagctgg ccggcgacgt gctgcggccc gacgatccgc tggcggtcgt gggcagcggg
     3901 tttctggtgc tcggtccgta cgacctgacc gcttacaaca acggcacgcc cgacatgcgg
     3961 gcctttgccc gcgaggaaga gctggagggc ctcgtcgcgt gcgtttgcca gaccttcctg
     4021 ggactgacgg tcaactgcag ccgctgccac gaccacaagt tcgacccgat tcggcagacc
     4081 gagttctatc agatctcggc cgcgatcggc gggacctatc aggctgacga gcgcgagtgc
     4141 cgctcagcaa gtggcaaatc cgcagccgag gagcggatcg ccaaactgcg ggaggagatc
     4201 gcggctctgg acggcaagcc ggatctcgcg gcgcagcgct cgcgcctgga gagcatcatc
     4261 cggctgctgg aaggcgggcc ggcccacgtc accgcgccgc agcagccgaa gccttggcga
     4321 gtgctggccc ggggcgactt ccgccagccg ggcgaggtgg tcgcgccgcg cgggatcgct
     4381 gcggtgccgg gagtcagcgc ggattggaga ctgccagccg acgcgccgga agccgagcgc
     4441 cgcaaggcgc tggccgagtg gatcgccggc cgcggcaacc ctctcacgcc gcgggtgatc
     4501 gtcaatcgcc tctggggcca tcacttcggc gcggggctgg tgccgacgcc gagcgacttt
     4561 ggttttcagg gcggccggcc ctcgcatccc gatctgctcg actggctggc cagcgagctg
     4621 gtgcagccca gcgatggaaa accgtggagc ctgaagcgga ttcagcggct gatcgtgctc
     4681 tcggccacgt accggcaggc atcgcgattc aatgagcaag ccgcagccgt ggacgccgaa
     4741 aaccggttgc tgtggcgcca atcgccgcaa cgtctctcgg cggagacgtt tcgcgacgcg
     4801 ctgttggccg tcagcggcga gctcgaccgg aagctgggcg gccccggcta tcgcgacttc
     4861 accatttcca gcgccggcaa caacgagacc tacaccgtgt tcgatgccgt cgggccggag
     4921 cataaccgcc gcagcttgta ccggacgtgg gtgcggacgg gcacgagccc gctcttggac
     4981 ctgtttgatt gtcccgaccc ttcggtgccg acgccgcggc gaagcgtgac gagcacgccg
     5041 ctgcaagcgc tggcccagct gaacgacgct gcgatcgagc actacgcgca gaagttcgcc
     5101 gagcgactgc gccgcgagca tccgggccac gaagcgaccc aaatccgccg ggcctacgag
     5161 ctggcctttg cgcggcaacc gtcggccgac gaggccgcct tcggcaagca attcgcggcc
     5221 gaccacggcc tggcacagtt gtgcctggtt ttgttcaatg ccaacgagtt tttgtatgtg
     5281 gattgagtac ttgtttaacc cggagccgga ggcgacggcg gctacccgcg cacagcctgc
     5341 gcctccggct acgggttaaa cgaatttcat gactccggtc tatgaccatt ctttctccat
     5401 tcgatgtccc cgccgcgccc ggcccctccc gccgcgacct gctcggccag ttcgcgacgg
     5461 gcctgggcgg aatggcgctg ctggcgctgc tggcgcggga aggcgcggcc gccgcggtca
     5521 agggcgaggc cgacgatccg ccgccgcacc atccgcccaa ggccaaacgg gcgattcaga
     5581 ttttcctcca gggcggcctc agccaggtcg attcgttcga tcccaagccc gagctggagc
     5641 ggctgcacgg caagtcggtc cccggcgacg agaagcccgt ggcgttcatg ggcaaggtcg
     5701 gcctgttgca caaggcccac ttcgcgttcg ctcagcgcgg ccagagcggc ctgtgggtct
     5761 cggacctctt tccacagatc gccacggtcg cggacgagct gaccgtcatc cgctcgatgt
     5821 ggtcgggcac cggcaaccac acgcccgcga cctacgaagc caacagcggc ttccgcacgc
     5881 tcggctttcc cgcggcgggc acctggattt cctacgggct gggctgcgag gtcgataatc
     5941 tgcccgcgtt tgtggtgctg cccgacagcc gctcgctgcc cacggggggc tcgaacaact
     6001 ggtcggccgg gtttttgccc gggcggcatc agggggtgat gtttcgcacg agcggaccag
     6061 cagtgcgcga cctgcgcccg gcgtcgggcg tgagcgaagc gacgcaggcg gcccgctatg
     6121 ccgcgatcgc cgagctgaac cgccgccacc tggccgagcg cggccgcgac gacgccctgg
     6181 agagccggct gcggagctac gagctggcgg cgcggatg
//
LOCUS       DASRJB010000216        11621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_345436, whole genome shotgun sequence.
ACCESSION   DASRJB010000216 DASRJB010000000
VERSION     DASRJB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345436"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFB80_05955"
     CDS             <1..286
                     /locus_tag="VFB80_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN33341.1"
                     /translation="HQKDPQWNWHYEFAFGRRPAEELYDLAKDPDQVRNVAAEPAYAN
                     AKKELAERLQKLLVDAGDPRVTGDGKTFDQPPFTDLPAPAEKAKKKKAAK"
     gene            358..1659
                     /locus_tag="VFB80_05960"
     CDS             358..1659
                     /locus_tag="VFB80_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33342.1"
                     /translation="MPVAFVLLLLAANPAADHWPQWRGPLQTGVSLSADPPQTWDAEG
                     TNVRWRTPLSGRGHSTPIVWGDRIFVTAAIPIGPAMSPRPSTAPGNHDNSPVTHKQRF
                     VAIAINRADGKVLWTKTLREAVPTEQGHRTASLASGSPVTDGERLYVLFGSFGLYCLD
                     FQGEVLWQRDFGPLQSLHGHGEGSSPVLYGDKLLINWDHEGKSFAAALDKRTGEPIWK
                     VDRTEVTSWATPIVVEHAGKPQVIISGTSRIRGYDLATGKVLWECGGLSANVVASPVS
                     DGEMVFAGSSYDKRALLAIRLAGATGDITDSRDQIAWSRFRGTPYVPSPLLYEGALYF
                     LTHYQGILSRVDGKTGEDRPGAVRLGDIGDIYASPVAAAGRVYVTDLNGATAVISSGE
                     IPRLIAINRLNEPVAASAALSGRELFLRGEKHLYCIAERAK"
     gene            complement(1665..3305)
                     /locus_tag="VFB80_05965"
     CDS             complement(1665..3305)
                     /locus_tag="VFB80_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN33343.1"
                     /translation="MERFRNVLLDVWREACRHIEIHESASTLAPMLSQHLPLACLLVR
                     RFDVPQHSLDTVAVGQPSAGYWHAAMRTTLTPARFRRLMAWANAGELLHGQRPDRTGD
                     LAALVPPEIEGEVLAGPLGVARNPSVAVEPAGALVLVAAHLKSFKAEHLQMAEALLEP
                     FSAALENDRRLHELAALREAAEADRRSLLSRLGRQEMSETIVGAEVGLRHVMERVVLV
                     AHSDVPVLILGETGTGKEVVARAIHTRSARGAAQPAARNTGPFIRVNCGAIPPDLIDS
                     QLFGHEKGSFTGAADTRKGWFERADGGTLFLDEIGELPLPAQVRLLRVLQDGFIERVG
                     GQQSIRVDVRIVAATHRDLAAMVKNGTFREDLWYRINVFPILLPRLRERTEDIPALVR
                     HFAEKAANRFGLPLALPTAGDLHVLMSYSWPGNIRELGAVIDRAAILGNGQRLEVTKA
                     LGSGTNPYVSVPASNSPTLYEVIPEATQPLAPAAAPEGPVVTLDAAMSQHIERALAAT
                     SGRIEGRGGAASLLGINPHTLRARMRKLRIDWSRFRRR"
     gene            3535..4593
                     /locus_tag="VFB80_05970"
     CDS             3535..4593
                     /locus_tag="VFB80_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN33344.1"
                     /translation="MSLRPLLLLPWLALAAGCYSGSPGTTVELLNVSYDPTREFYQEF
                     NESFAAYWKQQSGQEVSIEQSHGGSGKQARAVMDGMQADVVTLALAYDIDVIAQKAKL
                     LPADWQSKLPHHSSPYTSTIVFLVRKGNPKGIKDWGDLAKGDVEVITPHPKTSGGARW
                     NYLAAWGFALKQELGDLAKLKDPAAAAEVKAAHAKAKAFVEAIYKRVPVLDPGARGST
                     NTFVQRGIGDVLLAWENEAFLAVKGGAKDVEMVVPSISILAEPPVAVVESFAEKHGTT
                     QIAKAYLEHLYSPQGQQLAARHFYRPAIAESVSAEQRKQFAQLELFRIDDVFGGWAKA
                     QREHFDDGGTFDQISKVK"
     gene            4605..5444
                     /gene="cysT"
                     /locus_tag="VFB80_05975"
     CDS             4605..5444
                     /gene="cysT"
                     /locus_tag="VFB80_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZN33345.1"
                     /translation="MRFNFKQHSVLPGFGLTLGCTLLYLSLVVLVPLSLLFFRTATMP
                     LVDFWRVVTEPRVLASYKLTFGASLIGALVNVAFGFIVAWTLVRYRFPGRQLLDALID
                     LPFALPTAVSGIALTAIFAKTGWLGRFLYPLGIESAFSQLGVTIALTFIGLPFVVRTL
                     QPALEELDAEVEEAAASLGASRWQTFYRVILPTVLPAVLTGFALAFARALGEYGSVVF
                     ISGNKALQTEITSLLIITKLEQYDYAGATAIAAVMLVASFAILLGINTLQWWVGRARL
                     KGV"
     gene            5447..6364
                     /gene="cysW"
                     /locus_tag="VFB80_05980"
     CDS             5447..6364
                     /gene="cysW"
                     /locus_tag="VFB80_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZN33346.1"
                     /translation="MAAAASKPRRKNDPLWLQILMIGVSLAFVGVLLVLPLAVVFAEA
                     LSGGVSACFAALSKPAAWQAIKLTLLAAGISVPLNLVFGVVASWAIAKFEFRGKSVLI
                     SLIDLPFAVSPVISGLIYVLLFGLQGLLGKTLAAWNIEIIFAVPGVVLATLFVTFPFV
                     ARELIPLMQEQGDEEEQAAIALGASGWQTFWRITLPNIKWGLLYGVILCNARAMGEFG
                     AVSVVAGHRGRTNTIPLYVELLYNEYNAPAAFTMASLLALLGLITLAAKTWLEWKIHP
                     HTLTDGGPVATPLPPPANFEPTAAEVYSP"
     gene            6361..7428
                     /locus_tag="VFB80_05985"
     CDS             6361..7428
                     /locus_tag="VFB80_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HZN33347.1"
                     /translation="MSIEVNHVTKSFGIYKALDDVSLKVEDGELMALLGPSGSGKTTL
                     LRIIAGLEIPDENAQTAILFHGEDAARRQVAERQVGFVFQHYALFKHLSVFENIAFGL
                     RVRPRSTRPTNGQIRDKVHELLKLIQLENMGRRYPSQLSGGQRQRVALARALAIEPKV
                     LLLDEPFGALDAKVRQGLRNWLRRLHDELHMTTILVTHDQEEALEVADRVVVMNQSRI
                     EQVGTPADVFHHPASEFVMDFLGQVNVFHGRIEEGQAVLSGLAPASAAAGPQRGHLYV
                     RPHELDIQRYLNGTPAIPALVARINRAGALAKVALTTQDGGDIQVDLPLERLEQLGLQ
                     PGETVFVSAKKARVFTPDYVI"
     gene            7486..7677
                     /locus_tag="VFB80_05990"
     CDS             7486..7677
                     /locus_tag="VFB80_05990"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HZN33348.1"
                     /translation="MSLPESNLPSSAASNAERDEELTQIRDALRGLRFGAVNVIVQDG
                     VVVQIDRTEKRRVRRSDAK"
     gene            7883..8143
                     /locus_tag="VFB80_05995"
     CDS             7883..8143
                     /locus_tag="VFB80_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN33349.1"
                     /translation="MITIPVRSVDPASQDLLQRVALFLQQMRLASSSRLKLEASGGVV
                     TLAGEVPTFHERQRIYAASRRVAGVVQVIDRLEVSAPAASLT"
     gene            8187..8621
                     /locus_tag="VFB80_06000"
     CDS             8187..8621
                     /locus_tag="VFB80_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33350.1"
                     /translation="MSFCYFKLRCCSLIALALPALAGCGNEAQRTPVFKTVGSITFRN
                     QPAVGAFVVLHPKGATAGTPRPTAHVKADGTFEATTFETADGAPLGEYIVTVQRRKLV
                     KQNGDWTPGPNLIPDRYADPATSHLTIRIAEGENNLEPIVLR"
     gene            8690..9649
                     /locus_tag="VFB80_06005"
     CDS             8690..9649
                     /locus_tag="VFB80_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33351.1"
                     /translation="MPRSRPGFTLIELLVVIAIIGILVALLLPAVQAAREAARRSQCS
                     NNLRQLGLAAQSFHDAHNKLPSSVRPSGLTTLPRIAGLTLALHYIEQGNSYAKYDQTK
                     NWFDPQNLPITQAPISTYVCPSTPEPKRRDGVPEGNPWIGNLVAITDYSPTIGVDQRL
                     RSAGLVDAAGPGAMPKNGEPRLADVTDGLSNTIFYGESAGRPFVYRQNKKFGSLPAQR
                     VNAGGWSRPASDFSIDGSSFDGATLPGPCAINCTNGEDFGSTAFPHPYYGTEGTAEAY
                     AFHPGGANFAFGDGSVRLLAQALSIRDFARLVTRGEGEAIAAP"
     gene            complement(9997..11055)
                     /locus_tag="VFB80_06010"
     CDS             complement(9997..11055)
                     /locus_tag="VFB80_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HZN33352.1"
                     /translation="MQLKLHAFDLPLRHTFTISRESITSQPTLIVELSQGGVSGYGEA
                     TSNKYYGFTIQRMARDLAALAPQLAGERLDDPTALWARCQPQLTADPFALCALDQAAH
                     DLRGKLRGQPTWKLWGLSTEKTPASDYTIGIDAIDVMVAKLNEFPGWPIYKIKLGTDR
                     DLEIVRALRQHTSATFRVDANCGWTADQTIANSTELKKLGVEFIEQPLKPQLPEADHR
                     RVREQSALPIIADESCIVESDVSKCRGLFHGVNIKLVKCGGLTPARRMIAEARRLGLQ
                     VMVGCMTESTVGISAIAQLLPLLDYVDMDGAVLLAEDVATGVTLDRGRCIFPSVPGNG
                     VTLTRTGGNPFPDPTHHG"
     gene            complement(11055..>11621)
                     /locus_tag="VFB80_06015"
     CDS             complement(11055..>11621)
                     /locus_tag="VFB80_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33353.1"
                     /translation="ADKSVTLGFTQEDVRPSATRLLPHSAAVLAVLWLPQSDVVLSAG
                     VDQTIRLIDPARGETVRSFENHTGAVRDLALRPGDQEGPPLVASAGADRTVRFWQPTI
                     GRLVRFARLPVSPTAICWTVTGSHVLAACEDGRLRAVDPSTVEVVDLPDKLDGWAYSV
                     IATPDGKLAVLAGERGRLRVVSLSAINP"
BASE COUNT         2010 a         3846 c         3722 g         2043 t
ORIGIN      
        1 ccaccagaaa gaccctcagt ggaactggca ctacgagttt gccttcggca ggcgcccggc
       61 cgaggagctt tacgacctgg ccaaagaccc cgaccaggtc cgcaacgtcg ccgccgagcc
      121 ggcctacgcc aatgcgaaga aggaacttgc cgagcggctg cagaagctgc tggtcgatgc
      181 cggtgacccc cgcgtcaccg gcgatggcaa gacctttgac cagccaccct ttaccgacct
      241 gcccgcgccg gccgagaaag ccaagaagaa aaaggcggcg aagtgagaac agaggacctg
      301 gccgcaacgt attgcgctta cctaattctg aattcttcct gctgaattct gaatttcatg
      361 cccgttgcat ttgtactgct cctgctcgcc gccaatcccg cggccgacca ttggccgcag
      421 tggcgcgggc cgctccagac gggagtgtct ctctcagccg atccgccgca gacgtgggac
      481 gccgagggaa cgaacgtccg ctggaggacg ccgctgagcg gccgcgggca ttcgacgccc
      541 atcgtttggg gcgaccggat cttcgtaacg gcggcgattc cgatcggccc cgccatgtcg
      601 ccgcggccca gcaccgcgcc gggtaaccac gacaactcgc cggtcacgca caagcagcgg
      661 ttcgtcgcca tcgcgatcaa ccgggccgac ggcaaggtgc tctggaccaa gacgctgcgc
      721 gaagcagtgc cgacagaaca ggggcatcgc acggccagcc tggcttccgg ctcccccgtg
      781 accgacggcg agcggctgta cgtgctgttc ggctcgttcg ggctctattg cctcgacttc
      841 cagggcgagg tgctgtggca gcgcgatttt ggcccgctgc aatcgctgca cggacacggc
      901 gagggcagct cgcccgtgct gtatggcgac aagctgctca tcaactggga ccacgagggc
      961 aaatcgttcg cagccgcgct cgacaagcgg acgggcgagc cgatctggaa ggtcgatcgc
     1021 accgaagtga cgagctgggc cacgccgatc gtcgtcgagc acgccggcaa gccgcaagtc
     1081 atcatcagcg ggaccagccg gattcgcggc tacgacctgg cgacaggcaa ggtgctgtgg
     1141 gagtgcggcg ggctgtcggc gaatgtagtc gcgtcgcccg tttccgatgg cgagatggtc
     1201 ttcgccggca gcagctacga caagcgagca ctcttggcga tccgcctggc cggggcgacc
     1261 ggtgacatca ccgactccag ggaccagatc gcctggagcc gcttccgcgg gacgccctat
     1321 gttccttcgc cgctcttgta tgaaggcgcg ctctattttc tgacccacta tcagggcatc
     1381 ctgtcacggg tcgatggcaa gaccggcgag gaccgccccg gcgcggtgcg gctaggggat
     1441 ataggcgaca tctacgcttc gcccgtcgcc gccgccggcc gcgtctatgt caccgatcta
     1501 aacggcgcga cggccgtcat ctccagcggc gagatcccgc gattgatcgc gatcaaccgc
     1561 ctgaacgagc cggtcgccgc ctccgccgca ctgtctggcc gcgagttgtt tttgcgaggc
     1621 gaaaagcacc tgtactgcat cgccgagcgc gcgaagtaag tcgctcatcg ccgccggaag
     1681 cgcgaccaat cgatgcggag cttccgcatc cgggcgcgaa gcgtgtgcgg attgatcccc
     1741 agcaagctgg ctgcgccgcc gcggccttcg attcgcccgc tggtggcggc cagggcccgc
     1801 tcgatgtgct gcgacatggc ggcgtcgaga gtcacgacgg gaccttccgg cgccgcggcg
     1861 ggcgcgagcg gctgagtggc ctccgggatc acctcgtaaa gcgtggggga attcgacgcc
     1921 gggaccgaaa cgtacgggtt cgttccgctg cccagtgcct tggtaacttc cagccgctgg
     1981 ccgttgccga gaatcgccgc ccggtcgatc accgcgccca gttcgcggat gttgcccggc
     2041 caggaatagc tcatgagcac gtgcaggtcg cccgcggtcg gcaaggcgag cggcaggcca
     2101 aaccggttgg ccgccttttc cgcaaagtgc cgtaccaggg cggggatgtc ctcggttcgc
     2161 tcgcgcagcc gcggcagcag gatgggaaag acgttgatgc gataccagag gtcctcgcga
     2221 aaggtgccgt ttttcaccat cgccgccagg tcgcggtgcg tggcggcgac gatccgcaca
     2281 tcgacccgga tcgattgttg cccgccgacc cgctcgatga agccgtcctg caaaacgcgg
     2341 agcagtcgga cctgggccgg cagcggcaac tcgccgatct cgtcgagaaa cagggtgccg
     2401 ccgtcggccc gctcgaacca gcctttgcgg gtgtcggctg ctcccgtgaa gcttcccttt
     2461 tcgtggccga agagctgcga gtcgatcagg tccggcggga tcgcgccgca gttgacgcgg
     2521 atgaacggcc ctgtatttct ggcggcgggc tgcgctgctc cgcgggccga gcgggtgtgg
     2581 atcgcgcggg ccacgacttc cttgccggtg cccgtctcgc ccagaatcag caccggcacg
     2641 tcggagtggg ccaccagcac gacgcgctcc atgacgtgcc gcagtcccac ctcggccccg
     2701 acgatcgtct cgctcatttc ctgccggccg aggcgcgaca ggagcgagcg gcggtcggct
     2761 tcggccgctt cgcgcagcgc cgccaactca tggaggcggc ggtcgttctc cagcgccgcc
     2821 gagaagggct ccagcaaggc ctccgccatt tgcaggtgct cggccttgaa cgacttcaga
     2881 tgggcggcga cgagcaccag cgcgccggcg ggttcgacgg ccacgctggg attgcgggcg
     2941 acgcccagcg gccccgcgag aacttccccc tcgatttccg gtggcaccag cgctgccaga
     3001 tcaccggtgc gatcgggacg ctggccgtgc agtaattcgc cggcattcgc ccaagccatc
     3061 agccggcgga agcgggccgg tgtcagcgtc gtgcgcatcg cggcatgcca atagccagcc
     3121 gacggttgac cgacggccac cgtgtcgagg ctgtgctgcg gcacatcgaa tcgccggacc
     3181 agcaggcacg ccagcggcag atgctgcgac agcatcggag ccagcgtgga agccgattcg
     3241 tgaatctcga tgtgccggca ggcttcccgc cagacatcca gcaggacatt gcgaaagcgt
     3301 tccaagggcg acagttcccg tgatgggcaa tgggctgccg tgaaataaca cggcacgaac
     3361 ccagcatatt tcacggacta atggacggca accacagttt cccgcgtttt gcaagggttt
     3421 cgccaccggc gcggtcgatc ctacgtccgg cacgaccgtt gcgttcgcac tctggccgcg
     3481 ccttgcatgg agcgaggcta gcgcccgcaa ctgtcaactt taaccacttc agatatgtct
     3541 cttcgccccc ttttactcct gccctggctg gccctggccg ccggctgcta ctcaggttcg
     3601 cccgggacca ctgtcgagct gctcaatgtc tcgtacgatc cgacccgcga gttctatcag
     3661 gaattcaacg agtcgtttgc cgcctattgg aagcagcagt cggggcagga agtctcgatc
     3721 gagcagtcgc acggcggttc cggcaagcaa gctcgagccg taatggacgg aatgcaggcc
     3781 gacgtggtga cgctcgccct ggcctacgac atcgatgtga ttgcccagaa ggccaagctg
     3841 ctgccggccg actggcagag caagctgcct caccacagca gcccctacac ttccacgatc
     3901 gtgttcctcg tccgcaaggg caaccccaaa gggatcaagg actggggcga cctggcgaag
     3961 ggggacgtgg aggtcatcac accgcatccc aagaccagcg gcggtgctcg ctggaactac
     4021 ctggccgcct ggggttttgc gctcaagcaa gagctgggcg acctggcgaa actcaaggac
     4081 cccgcggccg cagccgaagt caaggccgct cacgccaagg cgaaagcgtt cgtcgaggcg
     4141 atctacaagc gcgtgccggt gctcgatccc ggcgcgcgcg gctccaccaa cacgttcgtc
     4201 cagcgcggca tcggcgacgt gctgctggcc tgggaaaacg aagccttcct ggcggtaaaa
     4261 ggcggcgcga aggacgtgga gatggtcgtg cccagcatca gcatcctggc ggagccaccg
     4321 gtggcggtcg tcgagtcctt cgccgaaaag cacggcacca cccagatcgc caaggcgtac
     4381 ttggagcact tgtattcgcc gcagggccag cagctcgcgg ccaggcattt ctatcgcccg
     4441 gcgattgccg aatcggtctc ggccgagcag cggaagcagt tcgcccagct cgagctgttc
     4501 aggatcgacg acgttttcgg cggctgggcc aaagcccagc gcgagcattt cgacgacggc
     4561 ggcaccttcg atcagatctc caaagtgaaa tagccgcccc tgccatgcga ttcaacttca
     4621 agcaacacag tgtattgcca ggcttcggcc tgacgctcgg ctgcacgctg ctctacctga
     4681 gcctggtcgt gctcgtcccg ctgtcgctgc tcttctttcg cacggcgacg atgccgctgg
     4741 tcgatttctg gcgggtcgtg acggagccgc gagtgctggc ttcgtacaag ctgacgttcg
     4801 gcgcctcgct catcggggcg ctcgtgaacg tcgcattcgg cttcatcgtc gcctggacgc
     4861 tcgtccgcta ccgttttccc ggccgccagc tgctcgacgc cctgatcgac ctgcccttcg
     4921 ccctgcccac ggccgtgtcg ggcatcgccc tcacggcgat ctttgccaag accggctggt
     4981 tgggccggtt cctgtatccg ctgggcattg aatcggcgtt ttcgcagctc ggcgtcacga
     5041 tcgccctgac gttcatcggc ttgcccttcg tcgtccgcac tcttcagccc gcattagaag
     5101 agctcgacgc cgaagtcgag gaagccgcgg ccagcctggg cgcgagccgc tggcagacgt
     5161 tctatcgcgt catcctgcca acggtgttgc cggccgtgct gacgggattc gcgctcgcct
     5221 tcgctcgggc cctgggcgaa tacggttcgg tcgtgttcat ctccggcaac aaggcgctgc
     5281 agaccgagat cacgtcgctg ctgatcatca ccaagctcga acagtacgac tatgccggcg
     5341 cgaccgcaat cgccgccgta atgctcgtgg cgtcattcgc gatcctgctg ggcatcaaca
     5401 cgctccagtg gtgggtcggc cgggcccggc tgaagggagt ctagccatgg cggccgccgc
     5461 ttccaaaccg cgacgcaaga acgacccact ctggctgcaa atcctgatga tcggcgtttc
     5521 cctggccttc gtcggcgtgc tgctggtgct gccgctggcc gtcgtgtttg ccgaggccct
     5581 gagcggcggc gtctcggcct gtttcgccgc gctaagcaag ccggcggcct ggcaggcgat
     5641 caagctgacg ctgctggcgg ccggtatctc ggtgccgctg aacctggtgt tcggcgtggt
     5701 tgcctcgtgg gcgatcgcca agttcgagtt ccgcggcaag agcgtgctga tctcgctgat
     5761 cgacctgccg tttgccgtat cgccggtcat ctcggggctg atctacgtgc tcctgttcgg
     5821 cctgcagggg ctgctgggca aaacgctggc ggcgtggaac atcgagatca tctttgcggt
     5881 gccgggcgtc gtgctggcca cgctgtttgt gaccttcccg ttcgtggccc gcgagctgat
     5941 cccgctcatg caggagcagg gggacgagga agagcaagcc gcgatcgccc tgggcgcgag
     6001 cggctggcag acgttttggc ggattacgct gccgaacatc aagtggggct tgctgtacgg
     6061 cgtcatcctg tgcaacgccc gtgcgatggg cgagttcggc gcggtctcgg tcgtcgccgg
     6121 ccaccgcggc cgtaccaaca cgatcccgct ctacgtcgag ctgctctaca acgaatacaa
     6181 cgcgcccgcg gcgttcacga tggcgtcgct gctggccctg ctgggcctga tcaccctggc
     6241 cgcgaaaacg tggctggagt ggaagattca cccgcacacg ctgaccgacg gcggcccagt
     6301 ggcaacgccg ctcccgccgc ccgcgaactt tgagccgaca gcggcagagg tttacagtcc
     6361 atgagcatcg aagtcaatca cgtcaccaag tcattcggaa tctacaaggc gctcgacgat
     6421 gtcagcctga aggtcgagga cggcgagctg atggcgctgc tcgggccgtc gggctcgggc
     6481 aagacgaccc tgttgcggat catcgccggc ctggaaatcc ccgacgagaa cgctcagacc
     6541 gcgattctgt tccatggcga ggacgccgcc cgccggcagg tcgccgagcg gcaggtcggt
     6601 ttcgtctttc agcactacgc cctgttcaag cacttgagtg tattcgagaa catcgctttc
     6661 ggcctgcggg tccgcccgcg cagcacgagg cccacgaacg gacagattcg agacaaagtc
     6721 cacgagctgc tgaagctgat tcaactcgaa aacatgggcc gccgctatcc ttcgcagctc
     6781 tccggcggcc agcggcaacg cgtggcgctg gcccgggcgc tggccatcga gcccaaggtg
     6841 ctgctgctcg acgagccgtt cggggccctc gacgccaagg tgcggcaggg gctgcggaac
     6901 tggctccgcc ggctgcacga cgaattgcat atgactacga tcctggtcac gcacgaccag
     6961 gaggaggccc tggaggttgc cgaccgcgtc gtggtgatga accagtcgcg gatcgagcag
     7021 gtcggcactc ccgccgacgt gttccaccat ccggcgtcgg agttcgtgat ggacttcctc
     7081 ggccaggtca atgtgttcca cggccggatc gaagaggggc aggccgtgct cagcggcctg
     7141 gcgcccgcga gcgcggccgc tggcccgcag cgcggacacc tgtacgtccg cccgcacgag
     7201 ctggacattc agcggtacct caacgggaca ccggcgattc ccgcgctggt ggcccgcatc
     7261 aaccgcgccg gcgcattggc caaggtcgcc ctcaccacgc aggacggcgg cgatattcaa
     7321 gtcgatctgc cgctggagcg actggagcag ctgggattgc agccgggcga gacggtgttc
     7381 gtctcggcca agaaggcccg cgtgttcacg ccggactatg tgatctgagt ttgccacgaa
     7441 ttggcacgaa agtcgcaatg gagcggcagt cgaggagcat gtgccatgtc gctgcccgaa
     7501 tcgaatctgc cttccagcgc cgcgagcaat gccgagcgcg acgaagagct gacgcagatc
     7561 cgcgacgccc tgcgcggcct ccggttcggg gcggtgaacg tcatcgttca ggacggcgtc
     7621 gtggtgcaga tcgaccggac cgaaaagcgg cgcgttcgcc gcagtgatgc gaaatagttg
     7681 tggccgaccc cacttgggag gcccgaacca tagcgattgt tcgccgctga ccgcagtgcg
     7741 tggaggctgg cgttgccggg aatcggcgcc atgttccatt ccacgctgct tctgatcctg
     7801 cctcccggcg ccgtggattg ttgtcggcgc gtcagctgac accagtcact tctatctcca
     7861 gtacacactt caggatcgcg ctatgattac cattcccgtt cgttccgttg accccgcgtc
     7921 gcaagacctg ctgcagcgcg tggcgctgtt cctgcagcaa atgcggctgg cttccagcag
     7981 ccggctgaag ctcgaagcat cgggcggcgt cgtcacgctg gccggcgaag tgcccacctt
     8041 ccacgagcgg cagcggattt acgccgccag ccgccgcgtc gcgggcgtcg ttcaggtcat
     8101 agatcggctg gaggtctcag cgccggccgc ctcgctgact tgatgatccg cccttatcca
     8161 tccaccccat tcacgaggag gatctcgtgt cgttctgcta tttcaagctc cgctgttgtt
     8221 cgctcatcgc gctggcccta ccggccttgg ctggctgcgg taatgaggcc cagcgcacgc
     8281 ccgtcttcaa gacggtcgga agtattacgt ttcgtaatca gccggcggta ggcgcctttg
     8341 tggtcctgca tcccaaagga gccacggcgg gaacgccgcg gccgactgcg cacgtgaagg
     8401 ccgacggcac gttcgaggcg acgacattcg aaacggcgga cggtgcgccg ctcggcgaat
     8461 acatcgtcac ggtccagcgg cgcaagctcg tcaagcaaaa cggcgactgg acgcccggcc
     8521 cgaatctgat tcctgatcga tacgccgacc cagcgacttc ccatctcacg atccgtattg
     8581 ccgagggtga gaacaacctg gagccgatcg tgttgcggta gcgaagaaac aaccgcaggc
     8641 ccgaatcctt aacctgaatt ctgaaatccc aatccgaaag gagtctccca tgccccgttc
     8701 ccgtcccgga ttcactttga tcgagctgct ggtcgtgatc gcgatcatcg gcatcctcgt
     8761 cgcgctgctg ttgccggcgg tgcaggctgc ccgcgaggcg gcccgccggt cgcaatgcag
     8821 caacaacctg cggcagcttg gactcgccgc gcagtctttc cacgacgcgc acaacaagct
     8881 gccttcgagc gtccgcccgt cgggcctgac cacgctgccg cgcatcgccg gcctgacgct
     8941 ggccctgcac tacatcgagc agggcaactc ctacgccaaa tacgaccaga caaagaactg
     9001 gttcgatccg cagaacctgc cgatcacgca agcgccgatt tcaacctatg tttgcccctc
     9061 gacgcccgag cccaagcgtc gcgacggcgt gccggaaggt aacccctgga tcggcaacct
     9121 tgtggcgatc accgactaca gcccgacgat cggcgtcgat cagcggctgc gctcggccgg
     9181 actggtcgat gccgcggggc ccggcgcaat gcccaagaac ggcgagccgc ggctggccga
     9241 cgttaccgac ggcctctcga acacgatctt ttacggcgaa tcggccggcc ggccctttgt
     9301 ctatcgccag aacaagaagt tcggcagcct gccagcgcag cgggtcaatg ccggcggctg
     9361 gtcgcggccc gccagcgatt tcagcatcga cggctccagc ttcgacggcg ctacgctgcc
     9421 cgggccctgc gcgatcaact gcaccaacgg cgaggacttt ggctcaaccg ccttcccgca
     9481 tccgtattac ggcacggaag gcacggccga ggcctatgcc tttcatccgg gcggagccaa
     9541 ttttgctttc ggcgacggct ccgtgcgact gctggcccag gccctttcga tccgcgactt
     9601 tgcccggctg gtgacccgcg gcgaaggcga ggcaatcgcc gcgccgtgac gcccggcgag
     9661 cgattccaaa agtttttccc tgacatccaa gaatcggcga gtttaaccga aaccctattt
     9721 gtggacaggt gtttaattag agcgttggag cgtctgcgct cccacgcctc cgatctgcca
     9781 tggccgggat ggactatagt ggctcacctg ctgtgcgctc caagaagctg gcctgttcaa
     9841 cgcgagcata gaggcctact aactcttata ttcctaatat ggactaagag accagcaatt
     9901 catcctatgt tgtctttcta caaagacttg cgccatttct cgattgctga aggcggatgc
     9961 gacgcgattc ccagacatca cccgattagg gcctgttcag ccatggtgcg tagggtcagg
    10021 aaagggattg ccgccagtgc gggtaagcgt gacgccatta ccggggacac ttggaaagat
    10081 gcatcgtccc cggtccagcg ttacgcccgt ggcgacgtct tcggccaaga ggaccgcgcc
    10141 gtccatatcg acgtagtcca agagcggcag caactgggcg atcgccgaga tgccgacggt
    10201 tgattcggtc atgcagccga ccatgacttg caagccgaga cggcgggcct cggcgatcat
    10261 ccgccgcgcc ggcgtcaggc cgccgcactt gacgagctta atattgactc catgaaacag
    10321 tccgcggcac ttgctgacgt cgctttcgac gatgcagctt tcgtcggcga tgatcggcag
    10381 cgccgactgc tcgcgaacgc ggcggtgatc cgcctcgggc agttgcggct tcagcggctg
    10441 ctcgatgaac tcgacgccga gttttttcag ttcggtgctg ttggcgatgg tctgatcggc
    10501 ggtccagccg caattggcat cgacgcggaa cgtcgcggaa gtgtgctgcc gcaacgcccg
    10561 cacaatctcc aaatcgcggt cggtgcccag cttgatcttg tagatcggcc agccgggaaa
    10621 ttcgttcagc ttggcgacca tcacgtcgat cgcatcgatg ccgatcgtgt agtccgaggc
    10681 cggcgttttt tccgtcgaaa ggccccacag tttccacgtc ggctggcccc ggagcttgcc
    10741 ccgcaagtcg tgcgccgcct ggtccagcgc gcacagcgcg aaaggatcgg cggtcaattg
    10801 cggctgacag cgggcccaca atgcggtcgg gtcgtcgagc cgctcgccag ccagctgcgg
    10861 cgcgagagcc gccaggtcgc gggccatccg ctggatcgtg aagccgtagt acttgttgct
    10921 cgtcgcctcg ccgtagccgc tgacgccgcc ttgcgacagt tcgacgatca gcgtcggctg
    10981 cgacgtgatc gactcgcggc tgatggtaaa cgtgtgccgc agcggcaggt cgaaagcgtg
    11041 gagtttgagt tgcatcacgg atttatcgca ctcaaactca ccacccgcaa ccgaccgcgc
    11101 tcaccggcca gaaccgccaa cttgccgtcc ggcgtggcga tgacggagta cgcccatcca
    11161 tcgagcttgt cggggaggtc gacgacttca acggtgcttg gatcgacggc ccgcagccgg
    11221 ccgtcctcgc aggcggccaa cacatgcgag cccgtgaccg tccagcagat cgccgtgggg
    11281 ctgacgggca gccgggcaaa ccggaccaag cggccgatcg tcggctgcca gaagcggacc
    11341 gtgcgatccg cgccggcgga ggcgaccagc ggcggccctt cctgatcgcc cgggcggagc
    11401 gccaggtcgc ggacggcgcc cgtatggttc tcgaacgagc gaacggtttc gccgcgggcg
    11461 ggatcgatca gccggatggt ctgatcgacg ccggcggaga gcacgacgtc gctctgcggc
    11521 agccagagca cggcgagcac ggctgccgag tgcggcaaga gccgcgtggc gctgggacgc
    11581 acgtcctctt gtgtaaagcc cagcgtgacc gatttgtccg c
//
LOCUS       DASRJB010000217         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_63110, whole genome shotgun sequence.
ACCESSION   DASRJB010000217 DASRJB010000000
VERSION     DASRJB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63110"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFB80_06020"
     CDS             <1..1117
                     /locus_tag="VFB80_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33354.1"
                     /translation="GPREVWRRLVGPGWSSFAVIGGAAFTQEQRGPSEAVVCFDVNTG
                     REHWSHLDTVRFEEIVAGAGPRATPTFHNGKLYALGGRGTLNCLDAATGKPFWSRDIL
                     KDAGREKPPEWGIASSPLIVQGLAIVFAGADPPNKDNPKPVKSVLAYDAESGDLKWFG
                     GKGIHSYSSPQLATIAGSQQVVIESDHGLEAFDPASGQPLWEYEWLIKDMFRVCQPVV
                     FDGQRVLVCTGMGGGTRQVTIAKEGEQWRATEDWASKDLEPYFNDFVLLGGYLYGFDG
                     PIFTCLDLSTGKKQWKKGRYGHGQVLLVGDAGLALIISDKGELVLAELTPKTLTERGR
                     IQALKGKTWNHPVLTASGKLLVRNGEEMACYDLSVRP"
     gene            complement(1247..2098)
                     /locus_tag="VFB80_06025"
     CDS             complement(1247..2098)
                     /locus_tag="VFB80_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33355.1"
                     /translation="MNAASLVIVLCSVLPGQAATAPQAQPASRSAKAVEAFRAEAAEY
                     VVRLASRPEEKLVLEKEAALRWDNPARTGEDGALFVWTLHGRPEMIGTIFTYRFKETL
                     SRKHEVHSLATGPLAAEFRGKQVWAPPKAGVVFQPVKGAPPPAAIPRQRLSQMKTLAR
                     DFAVSMTDAEGQQFQLRLLAQPLVRYEPQDKQVQDGALFAFSLGTDPEAILLLEARAT
                     KDAFEWQYAFARFHYIELKAQHQGREVWRVPALPPDIMNLDIGQARYQDSVYTTYHVE
                     RGIPVAE"
     gene            complement(2170..3114)
                     /locus_tag="VFB80_06030"
     CDS             complement(2170..3114)
                     /locus_tag="VFB80_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HZN33356.1"
                     /translation="MPLYMVLLAGGLVLGTDDVPTETSPAALAFDPRAVAVEFVDQSV
                     LKMVLADERIEIVTPHGRLQVPTDEIRRIEFAQRLPADAAARIDELIKQLASPDMAVH
                     EPAAAELVAAGGPAWLAVVKAAKSGGAELAPRATAVMKKLRPKLTKEELAAREDDLIE
                     TSDAKIAGKIATPQLKVRTAQFGELGLKIADARNLRHQSLLPRVIAEVASEEALPDPG
                     QLTGYQSKIGQTFAFRVTGAVAGGSIWGTDVYTTDSMLAMAAVHAGVLKAGETGVVRL
                     KIIASPPSFAGSFRNGVRTSPYGVYGGAYQILKDGADE"
     gene            complement(3177..4628)
                     /locus_tag="VFB80_06035"
     CDS             complement(3177..4628)
                     /locus_tag="VFB80_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33357.1"
                     /translation="MSGHSPQHEQHAFANYRARRRDGLAVVDRRGMLKAGIAGVAGLS
                     LPQLLRSRAAAASRGAAGKNKSCILLWMAGGPSHIDTWDSKPDRPQINRGPFGVTQTK
                     LPGVVICEHLPKQAAMLDRFTIIRSVDARHSNHEPNKVFQTGNSAAEGRTNPEAEKYP
                     ALASLIAKHHGANHLAMPPYVAFQKSRSHIAYAGYLGKQYDPLLAERAVALPVYTNVG
                     QDTGTIAGGEMFQFSAGLSSDRLHDRRALLEEFDRLRAGRDQDGSLAALNHYQQQAVD
                     MLLGRKAQEAFDLTREPQETRDRYGKHLWCQQALVARRLVEAGVAFVTIDLSYHTASG
                     TWDTHGDNIPPYGGIRRGLGPLLPLFDHLITTLVGDLQGRGILDDVLVLAMGEFGRTP
                     MMGTQDSTDGRNHWPHVMSMCLAGGGLKHGQVIGATETDGGAIRERPVTPADLAATLF
                     RYFGVPQDTSYLDNQGRPRVVIENDGQPLPELF"
BASE COUNT          754 a         1591 c         1569 g          770 t
ORIGIN      
        1 cgggccgcgc gaagtgtggc ggcggctcgt cggcccgggc tggtcgtcat ttgccgtgat
       61 cggcggcgcg gccttcacgc aggaacagcg cgggccgagc gaagccgtcg tctgtttcga
      121 cgtcaacacg ggccgcgagc actggtcgca cctggacacg gtccgcttcg aagaaatcgt
      181 ggccggtgcg ggcccacggg cgacgcccac gtttcacaac ggtaagctct acgccctcgg
      241 gggccgcggc acgctgaact gcctggacgc cgccacgggc aagcccttct ggtcgcgcga
      301 cattctgaaa gacgccggcc gcgagaagcc gccggagtgg ggcattgcca gctcgccgct
      361 aatcgtgcag ggcctggcca tcgtcttcgc cggggcggac ccgccgaaca aggacaaccc
      421 caagccggtc aaaagcgtgc tcgcctacga cgcggaaagc ggcgacctga aatggttcgg
      481 cggcaagggc atccacagct acagctctcc gcagctcgcg actatcgccg gctcccagca
      541 ggtcgtgatc gaaagcgacc acgggctgga agcgttcgac cccgccagcg gccagccgct
      601 gtgggaatac gaatggttga tcaaagacat gttccgcgtc tgccagcccg ttgtcttcga
      661 cggccagcgg gtgctcgtct gcaccggcat gggcggcggc acgcggcagg tcacgatcgc
      721 caaggagggc gagcagtggc gggccaccga agactgggcc agcaaggatc tcgagcccta
      781 cttcaacgac ttcgtcctgt tgggcggcta cctctacggc ttcgacggcc cgatcttcac
      841 ctgcctcgat ctttccactg gcaaaaagca gtggaaaaaa ggccgctacg gccacggcca
      901 ggtgctgctc gtcggcgacg cggggctggc cctaatcatc tcggataagg gcgagcttgt
      961 cctcgcagag ctcacgccca agacgctcac ggagcgcggc cgcattcaag ccctcaaggg
     1021 caaaacctgg aaccaccccg tcctcaccgc cagcggcaag ctcctggtgc gcaacggcga
     1081 ggagatggcg tgctacgacc tcagcgtgag gccgtagcgg tagggtggga caagcgcgag
     1141 caccgccgcg aagcgggggt cggccagcgc cggcccacca gcaagtcggt gtgccgccgg
     1201 ccggcgacca ctgcgcggtg gcggcctggg gtcacatgct acctgctcac tccgccaccg
     1261 gaatcccgcg ctcgacgtga tacgtcgtat agacgctgtc ctggtaccga gcttgcccga
     1321 tgtcgaggtt catgatgtcc ggcggcagcg cgggcacgcg ccagacttcg cggccctggt
     1381 gctgcgcctt cagctcgatg tagtggaagc gggcgaaggc atactgccac tcaaaggcgt
     1441 ctttggtcgc ccgcgcttcc agcagcagga tcgcttccgg gtcggtgccc agcgaaaacg
     1501 cgaatagcgc cccgtcctgg acctgcttgt cctgcggttc gtagcggacc agcggctgcg
     1561 cgagcaatcg caactgaaat tgttgtcctt cggcatcggt catgcttacc gcgaagtcgc
     1621 gggccagcgt tttcatctgc gagagccgct gccgcggaat ggcggccggg ggcggcgcgc
     1681 ccttgaccgg ttgaaacacg acgcccgcct tgggcggcgc ccagacctgc ttgccgcgga
     1741 actcggcggc cagcgggccc gtcgccagcg aatggacctc gtgcttgcgg ctgagcgttt
     1801 ccttgaagcg gtaggtaaag atcgtgccga tcatttccgg ccggccgtgg agcgtccaga
     1861 cgaacagggc tccgtcctcg cccgtccggg ccggattgtc ccagcgcagg gcggcttcct
     1921 tttcgagaac cagcttctct tccggtcggc tggccagacg cacgacgtac tccgccgcct
     1981 ccgcccggaa ggcctcgacc gccttggccg aacgcgacgc cggctgcgcc tggggcgcgg
     2041 tggccgcctg tcccggcagt acggagcaaa gcacgatcac gagcgaagca gcgttcatag
     2101 cgcggccctc cgaccgcatt aaagacacta tgtcttggca cgtcaacggc gcggctgcca
     2161 gaccgcggct cattcgtccg cgccgtcctt gagaatctgg tacgccccgc catagacgcc
     2221 gtaggggctg gtccgcacac cgttgcggaa gctgccggcg aacgaggggg gcgaggcgat
     2281 gatcttcagg cggacgacgc cggtctcgcc ggccttgaga acgccggcgt gtacggcggc
     2341 catcgccagc atggagtcgg tggtgtagac gtcggtgccc cagatcgaac cgccggcgac
     2401 ggccccggtg acgcggaagg cgaacgtctg gccgatcttc gattgataac ccgtcagttg
     2461 gcccgggtcg ggcagcgcct cctccgacgc gacctcggcg atcacccgcg gcagcagcga
     2521 ctggtgccgc aggttgcgag cgtcggcaat cttcagccca agctcgccga actgggccgt
     2581 gcggaccttg agctgcggcg tggcgatctt gccggcgatc ttggcgtcgc tcgtttcaat
     2641 caggtcgtcc tcgcgggcgg ccagttcttc cttcgtcagt ttgggacgca atttcttcat
     2701 gacagccgtg gcccgcgggg ccagctccgc cccgccgctc ttggcggctt tcacgaccgc
     2761 cagccaggcc gggccgcccg cggccaccag ttccgccgcg gccggctcgt gcacggccat
     2821 gtcggggctg gccagctgct tgatcagctc gtcgatccgc gccgccgcat cggcgggcag
     2881 gcgctgggcg aattcgatcc gccggatctc gtcggtcgga acctgcagcc tgccgtgcgg
     2941 cgtgacgatc tcgatccgct cgtcggccag caccattttc aggacacttt gatccacaaa
     3001 ctcgaccgcc acggctcgcg gatcgaacgc cagcgcagcc ggcgacgtct ccgtcggcac
     3061 atcatccgtg ccgagaacca ggccaccggc aagcagcacc atgtagagcg gcatcggtcg
     3121 atcctcctac cgagccatta tgccgtccca catccgccaa atgccatcgg tcccgtttag
     3181 aacaactcgg gcagcggctg gccgtcgttt tcaatcacga ctcgcgggcg gccttgattg
     3241 tcgagatacg acgtgtcctg cgggacgccg aaatagcgga acagtgtcgc ggcgaggtcg
     3301 gcgggcgtga cgggccgctc gcggatagcg ccgccgtcgg tctcggtcgc gccgatcacc
     3361 tgcccgtgct tcaggccgcc gcccgcgaga cacatcgaca tcacgtgcgg ccagtggttg
     3421 cggccgtcgg tgctgtcctg cgtccccatc atcggcgtgc ggccgaactc gcccatcgcc
     3481 agcacgagca cgtcgtccag gattccgcgg ccctgcagat cgcccacgag cgtcgtgatc
     3541 aggtggtcga aaagcggcag cagtggtccc aacccgcggc gaatgccgcc gtagggcgga
     3601 atgttgtcgc cgtgcgtgtc ccacgtgcca gaagccgtgt gataactgag atcgatcgta
     3661 acgaacgcca cgccggcctc gaccagccgg cgggccacca gcgcctgctg gcaccagagg
     3721 tgcttgccgt agcggtcgcg agtctcctgc ggctcgcgcg tcaggtcgaa ggcctcctgg
     3781 gccttgcggc ccaggagcat atcgacggcc tgctgctggt aatggttgag cgcggccagc
     3841 gagccgtcct ggtcgcggcc ggcccgcagg cggtcgaact cctcgagcag cgcccgccgg
     3901 tcgtgcagcc gatcgctgga aagcccggcg gaaaactgaa acatctcgcc gccggcgatt
     3961 gttccggtgt cctgcccgac gttcgtataa accggcagag cgacggcccg ctcggcgagc
     4021 agcggatcgt attgcttgcc caggtagccg gcataggcga tgtgcgagcg cgacttctgg
     4081 aacgcgacat acggcggcat cgccagatga ttcgccccgt ggtgcttggc gatgagcgaa
     4141 gcgagggccg gatacttctc ggcctcgggg ttggtccggc cctcagctgc tgagttgccg
     4201 gtctgaaaaa ccttgttcgg ctcgtggttg ctgtggcggg catcgaccga gcggatgatg
     4261 gtgaaccggt cgagcatcgc cgcctgcttg ggcaggtgct cgcagatcac gacgcccggc
     4321 agcttcgtct gcgtcacgcc gaaggggccg cgattgatct ggggccggtc gggcttgctg
     4381 tcccaggtgt cgatgtggct cgggccgccg gccatccaga gcaggatgca gctcttattc
     4441 ttgccggccg cgccccgcga ggcagccgcc gcccgagaac gcaacagctg cggcaaactc
     4501 aagcctgcca cgcccgcgat ccccgccttg agcatgccgc ggcgatcgac caccgccagg
     4561 ccgtcgcggc ggcgtgcccg gtagttggcg aacgcatgct gctcgtgttg cgggctgtgg
     4621 ccggacatga aggactccga agactgtggc accggcccca tcgtatcagt cgcagtttat
     4681 cgtt
//
LOCUS       DASRJB010000218        10021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1755024, whole genome shotgun sequence.
ACCESSION   DASRJB010000218 DASRJB010000000
VERSION     DASRJB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755024"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VFB80_06040"
     CDS             complement(<1..1559)
                     /locus_tag="VFB80_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33358.1"
                     /translation="MQVLTRFLVTAALLAAAACIWAQEPKLPPAADRPVDFVRDIQPL
                     LADRCNHCHGEDEQEGQLRLDAKVIVMRGGKSGPLLVKGKSAESLLVKRIAGLGTEKR
                     MPLEDEPLTGEQIGLIRAWIDQGAKWPDGVGSKATVVKKHWAYVAPNSPPLPALRDTG
                     WSQNAIDAFVLARLEKAGLAPSPAADRARLIRRASLDLIGLPPSVADVDEFLADQSPD
                     AYERLVDRLLASPRYGERWATPWLDAARYADSNGYQRDGHRTIWPYRDWVILALNADL
                     PFDQFTLEQIAGDLLPAATPKQQLDQQIATGFHRCTTVNVEAGTDEEENRTNQVIDRV
                     NVTGTVWLGTTLECCQCHNHKYDPFTQRDYYQFFAFFNSTPKETYQRTKGSAALDFGG
                     PEASLPVEPAVAKERQELASRRQVLAKELEQCLETEASGLADWEQKMQADKAELAKLP
                     ANIRRVINVAADKRNANQKKALRNYFVGLQPQIKKMQAEMDELQKQLDALAPTATLVM
                     SDLEQPRMTAIL"
     gene            complement(1943..2395)
                     /locus_tag="VFB80_06045"
     CDS             complement(1943..2395)
                     /locus_tag="VFB80_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33359.1"
                     /translation="MPEKNRILIADDNQPNRELLEAYLVGVECDVEHAVDGQDTLAKV
                     KSFKPDVILLDVMMPKLSGFEVCQKLKADPASRKILILMVTALNETGDIERAVRAGTD
                     DFVSKPVDKFGLLKRVETMLKLKDLTDENERLRRYIDEMEGRGPGSGT"
     gene            2610..5336
                     /locus_tag="VFB80_06050"
     CDS             2610..5336
                     /locus_tag="VFB80_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN33360.1"
                     /translation="MSQSAQTERPRADRPSRMTLAESAANGDRRFREMIDALPAAIYT
                     TDAEGRVNHFNPAAVLLSGRTPEVGSDCWCVTWKLYQPDGTPLPHEQCPMAVALRTGR
                     AVGRSEIIAERPDGTRVWIAAYPMPLLENGRVVGGINMLLDITDQKRAEEANARLAAI
                     VESSDDAIISKDLTGTITSWNRGAERIFGYTAAEAIGRPVTMLMPPERTDEEAGILER
                     IRRGERVDHYETVRRHKDGSLLDISLTVSPVRDAAGQIIGASKVARDITERKQAGQAV
                     HASEERYRTLFDLGPVAIYTIDTAGVIQKFNRQAAMLWGREPAVGDTDERFCGSFKMF
                     RPDGSHMPHELCPMADVVTGKLSEVRDGEVHIERPDGSRVVVVVNIRPLKNAEGEIVG
                     AINCFYDITERKQTEEQLRRNAETFAKLVEQAPFGIYVLDASFRIQQVSAGAQPAFRN
                     VRPLIGRDFSEALRIIWPEPLASRVIEIFRHTLQTGEPYVAPSLTERRRDVEAVESYE
                     WQIHRATLPDGQYGVVCYFFDATQLREAESALREADRRKDVFLAMLGHELRNPLSAIT
                     SAIQLSEMPNLGDSDRLWVRMTLARQTDQLGRLVDDLLDVARITRDRIQLKREPLLVA
                     DVVRRSVETTRPLVERARHKLTIALPQEPLWVLADPARLQQIVSNLLVNAVKYTPDGG
                     QISIRASREGNQVAIAVKDNGIGLSPQMQTQVFDLFTQAEQGIERAQGGLGIGLTLVR
                     RLVERHGGSVSVTSDGPQRGSEFTVRLPAIEPPPAAAARESPTGAEPARSHRILIVDD
                     NRDAGRTLSLLLKTLGHETALAFDGRQALEVAAEFRPAVALLDLGLPEINGFDLAVHL
                     KQGDPRIQLIAISGYGQAADRRRSQDAGFDRHFVKPVKIDELLSALNAVEPQ"
     gene            complement(5362..6618)
                     /gene="glyA"
                     /locus_tag="VFB80_06055"
     CDS             complement(5362..6618)
                     /gene="glyA"
                     /locus_tag="VFB80_06055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZN33361.1"
                     /translation="MNILQQQDPDVFAAIAAEEQRQQDGLEMIASENYTSPAVMQAVG
                     SVLTNKYAEGYPGRRYYGGCEHVDVIENLARDRVKRLFGAEHANVQPHSGSQANQAVY
                     FSVLEPGDTVLGLDLAHGGHLTHGMKLNLSGKLYKFHSYGVTKDAHRIDFDQVARQAR
                     EHKPKLIVAGASAYPREIPHEKFAEIAREVGAKLMVDMAHYAGLVAAGLHNNPVPVAD
                     FVTTTTHKTLRGPRAGTVLCKAEFAQAIDKTIFPGLQGGPLMHVIAGKAVCFGEALRP
                     EFQDYGRAIIANAQALAEVLLSGGLRLVSGGTDNHLMLVDVTPLSIGGKQATQALEHC
                     GITVNMNMIPFDTRKPLDPSGIRIGTPALTTRGMGPSEMQQIGRWMLQALQAPTDPAL
                     HHRIRGEVSSLSAQFPVPAAALAGTT"
     gene            6740..7510
                     /locus_tag="VFB80_06060"
     CDS             6740..7510
                     /locus_tag="VFB80_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZN33362.1"
                     /translation="MRVIPVIDLMNGVVVRGVAGRRQEYRPIRSKLVADSQPTAAARA
                     FAEHFGFDTAYVADLDAIQFRGDAPPYFTACYERIAACGLALWLDAGVGTAAAAQHLL
                     AAVEPLAMDVQFVIGLESLVSHLELAQIVAVLGRQRTMFCIDLEEGNLRTQIGRWAGA
                     DAVEIGFLAMAHGLSRLIVLDLADVGMNGGTRTLELCRRIRHTFGSAVELTAGGGVRG
                     GKDLLALEDAGCDAALVASALHDGRLTRAELDSLACRK"
     gene            7598..8137
                     /locus_tag="VFB80_06065"
     CDS             7598..8137
                     /locus_tag="VFB80_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33363.1"
                     /translation="MNTWNTFHDEAYTFPNLCAKCAAPDPTETWQLKYYEVEYPEGMP
                     WGPYDQKPRNYNTYLTDVPLCKECLGSVRWRSRYCWLAGGLAGVVAGIAMFGLISAHR
                     PAEPDLGFVPFGAGLFVALAVGYVLKAVFVDALCFAKFNGREQKLAFRNKEFQAQFDR
                     LNFQYSATTGKYADGGRAW"
     gene            8282..8764
                     /locus_tag="VFB80_06070"
     CDS             8282..8764
                     /locus_tag="VFB80_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33364.1"
                     /translation="MFSGPEIAATLAELDFTHVVWIPDSATGPWETALAAAPALRLVR
                     VCREGEAWPLAAGLHLGGQQPLVVLQTTGLFESGDALRNVLLDMRLPLYALIGVRSEL
                     VADSRDSARTFAEPILRAWGLDCVWLRAAADRTKFVEHVRRCRQEQKPGAALLAEGPP
                     "
     gene            8764..9399
                     /locus_tag="VFB80_06075"
     CDS             8764..9399
                     /locus_tag="VFB80_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN33365.1"
                     /translation="MQRKPHSGMPVADAVQVLIDHRASGQIVVTNQGAARLWPKLSRR
                     PLDLHYNPSTMGGAIPLALGLALAQTQREVLVVSGDGALLMSLGALVTVSGSGAANLT
                     VVVLDNGLYEVTGGQKTPATAAVDFAGLAKSAGFPSAYRFRDLAVWRGQAAAALSAAG
                     PRFISLEVEPMPPEYLRSPTPPLAEQIVEFRREVGGRVEISSVQSCCRAGS"
     gene            complement(9394..>10021)
                     /locus_tag="VFB80_06080"
     CDS             complement(9394..>10021)
                     /locus_tag="VFB80_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33366.1"
                     /translation="VALLARPLIVAFVALWHWDSLFALLLLFDPIQWAMLAVALFLAR
                     SALRSLRLMRTGSAPATIFEPLLRTNVARAAWIGCLCFTLLFSGFLGYEINQVWPAMI
                     GAIRQSIDDGQLLAGPQLPGCDSPQVVQLVNKLVRDLNIAPSISAIDGHRQISFDATA
                     QRRVGLCIAHTKPSAIPIVYTITWLDRDKRQFSVSVQIGSDLPPQTKS"
BASE COUNT         1654 a         3216 c         3340 g         1811 t
ORIGIN      
        1 agaatcgccg tcattcgcgg ctgctccagg tcgctcatga ccagcgtcgc cgtgggcgcg
       61 agcgcgtcaa gctgcttctg caactcgtcc atctcggcct gcatcttctt gatctgcggc
      121 tgcaggccga cgaagtaatt acggagcgcc tttttctggt tggcgttccg cttgtcggct
      181 gccacgttga tcacgcggcg gatgttggcc ggcagcttgg ccagttcggc cttgtcggcc
      241 tgcattttct gttcccagtc ggccaggccg ctcgcctccg tttccaggca ctgctcgagt
      301 tccttggcca ggacctgccg gcgggaggcg agctcttgcc gctctttggc gacggccggt
      361 tcgacgggca ggctcgcctc ggggccgccg aagtcgagag ccgccgagcc tttcgtccgc
      421 tgatacgtct ccttgggcgt gctgttgaag aaggcgaaga actggtagta gtcgcgctgc
      481 gtgaagggat cgtacttgtg gttgtgacac tggcagcact ccagcgtcgt gccgagccac
      541 acggtgcccg tcacgttcac gcggtcgatc acctggttgg tacggttctc ctcttcgtcg
      601 gtgcccgctt cgacattgac cgtcgtgcag cggtgaaagc cggtcgcaat ctgctgatcg
      661 agttgttgct tgggggtcgc cgcggggagc aggtcgccgg cgatctgctc gagggtgaac
      721 tgatcgaagg gcaggtcggc gttgagcgcc aggatcaccc agtcgcgata gggccagatc
      781 gtgcggtggc cgtcgcgctg gtaaccgtta ctgtcggcgt agcgggcggc gtcgagccag
      841 ggcgtcgccc agcgttcacc gtaccgcggt gaggccagca atcgatcgac cagccgctcg
      901 taggcgtcgg gcgattggtc ggccaggaac tcatcgacgt cggccacgct cggcggcaat
      961 ccgatcaggt ccaggctcgc gcggcgaatc agccgggcgc ggtcggccgc gggggacggc
     1021 gcgaggcctg ctttctccaa gcgggccagc acgaaggcgt caatggcgtt ttgcgaccat
     1081 cccgtgtccc gtaatgccgg cagcggcggc gagttcggtg cgacataggc ccagtgcttc
     1141 ttcaccacgg tcgccttcga ccctacgcca tcgggccatt tggcgccttg atcgatccag
     1201 gctcggatca ggccgatctg ctcgcccgtt agcggctcat cctccagcgg catccgcttt
     1261 tcggtcccca ggccggcgat ccgctttacg agcaggctct cggcgctctt gcccttaacc
     1321 agcaatggcc ccgatttgcc gccccgcatc acgatcacct tggcatccag gcgtagctgg
     1381 ccttcctgtt catcctcgcc gtgacagtga ttgcaacgat cggccaagag tggctgaatg
     1441 tcgcggacga aatcgacggg gcgatcggca gccggcggca gcttcggctc ctgggcccag
     1501 atgcacgcgg ctgccgccag gagtgctgcc gtaaccagaa accgcgtaag cacttgcatc
     1561 gccgctcgcc cccgccaggc tgttgacgca tccgattttt cgacgcctga gcctctcatt
     1621 ttattggctg gcagaggcga agctcaatcc gcgcatatgc gcgcatcgtg cgatgagaat
     1681 tctcgggagc gccagtgtct gacataagtc tttaggtatc caatagttac gaccgttttt
     1741 ctggaacggc gattatgcgc attatgcgcg cccgccatgt ccttcatcca cgtgcgacgc
     1801 gcttactgta ttctccacaa gcagtttcgt tgtggttgtc ggctatgcgc gagcctggca
     1861 gttatgcgca taacgtgcca agggcgtaac atcgattgta cactagttgc caataaatga
     1921 caacgcgccg gcgacgtttc ggctaagtcc cggagcccgg gccgcggcct tccatttcgt
     1981 cgatgtaccg ccgcagccgc tcgttctcgt cggtcaggtc cttgagcttg agcatggtct
     2041 cgacgcgctt cagcaggccg aatttatcga ccggcttgct gacgaaatcg tcggtgccgg
     2101 cccgcacggc ccgctcgatg tcgccggtct cgttgagggc ggtgaccatc aggatcagga
     2161 ttttgcggct ggccggatcg gctttgagct tctggcagac ttcaaacccc gagagcttgg
     2221 gcatcatcac gtcgagcagg atcacgtcgg gcttgaacga cttgaccttc gccaaggtgt
     2281 cctgcccgtc caccgcatgc tcgacgtcgc actcgacgcc gaccagatac gcttcgagca
     2341 attcgcggtt aggctggttg tcgtcggcga tcaggatgcg gtttttctcg ggcattgagc
     2401 ggcaattgaa cgagtagcac gggcagcaag cgtccctcgc ccttgtccgg cgcattgtag
     2461 cgaaaccgct gggcttgggc atcgcgattg tgctcggcag ccaattcgtg ccatctggtc
     2521 gccgcacgag gcgggctgct ctatattctg tgtagagcat gcttgtgtag aacgcggcgc
     2581 gccagagcgc cggagcgtgg agggcgttca tgagccagtc ggcgcagacg gagcggcccc
     2641 gggccgatcg gccatcgcga atgactcttg ctgagtccgc cgcaaacggc gatcgccgct
     2701 ttcgcgaaat gatcgatgcg ttgcccgcgg ccatctacac gacggacgcc gaaggtcgcg
     2761 tcaatcactt caaccccgca gccgtgctgc tctcgggtcg gacgccggaa gtgggaagcg
     2821 actgctggtg cgtaacctgg aagctctatc agcccgacgg cacgccgctg ccgcacgaac
     2881 agtgtccgat ggccgtcgcg ctgcgaaccg ggcgcgcggt gggcaggtcc gagatcatcg
     2941 ccgagcggcc cgacggcacg cgggtctgga tcgcggccta tccgatgccg ctcttggaga
     3001 acggccgcgt cgtcggcggc attaacatgc tgctggatat caccgaccag aagcgcgcgg
     3061 aggaggccaa cgcccggctg gcggccatcg tcgaatcgtc cgacgacgcc atcatcagca
     3121 aagatctcac cggcaccatc accagctgga accgcggggc cgagcggatc tttggctaca
     3181 ccgcggccga agcgatcggt cggccagtca caatgctcat gcctcccgag cggaccgacg
     3241 aagaggccgg catcctggag cggattcgcc gcggcgaacg ggtcgatcac tacgagacgg
     3301 tccgccgcca caaggacggc tcgctgctcg acatctcgct gaccgtctcg ccggttcgag
     3361 acgccgccgg gcagatcatc ggcgcatcga aggtggcccg cgacattact gagcgcaagc
     3421 aggcggggca agcggtgcac gcgagcgaag aacgatatcg cacgctgttc gacctggggc
     3481 cggtggccat ctacaccatc gacaccgcgg gggtgattca gaaattcaac cgccaggccg
     3541 caatgctatg gggccgcgag ccggcggtgg gggatacgga cgagcggttt tgcgggtcgt
     3601 tcaagatgtt ccgtccggac ggcagccaca tgccccacga gctgtgtccg atggccgacg
     3661 tggtgaccgg caaactctcg gaggtgcgtg acggggaagt acacattgag cgtccggacg
     3721 gctcgcgggt cgtcgtcgtc gtcaatattc gtccgctgaa gaatgccgaa ggcgaaattg
     3781 tcggagcgat caactgcttt tacgacatta ccgagcgcaa gcaaaccgag gagcagttgc
     3841 gccgcaacgc cgagacgttc gccaagcttg tcgagcaagc gccgttcggg atctacgtgc
     3901 tggacgcgag ttttcgcatc cagcaggtca gcgcgggagc gcagccggcg ttccgcaacg
     3961 tgcggcccct gatcggccgc gatttcagcg aagccctgcg catcatctgg cccgagccct
     4021 tggccagccg cgtcatcgag atcttccgcc acacgctcca aactggcgag ccgtacgtgg
     4081 cccccagcct gactgagcgc cgccgcgatg tggaggccgt cgagtcctac gagtggcaga
     4141 tccaccgcgc tacgctcccc gacgggcagt acggcgtggt gtgctacttc ttcgacgcca
     4201 cgcagctgcg cgaggccgag agcgccctcc gggaggccga ccgccgcaag gacgtcttcc
     4261 tggcgatgct cggccacgag ctgcgcaacc cgctgtcggc gatcaccagc gccatccagc
     4321 tctccgagat gccgaatctc ggcgacagcg accggctgtg ggtgcgcatg accctcgcgc
     4381 ggcagaccga ccagctgggc cgcctggtcg atgacctgct cgacgtggcc cgcatcacgc
     4441 gcgaccggat tcagctcaag cgggaaccgc tgctggtggc cgacgtggtc aggcgatcgg
     4501 tggaaacgac gcggccgctc gtcgagcggg cccggcacaa gctcacgatc gcgctgccac
     4561 aggagccgct gtgggtcctg gccgatccgg cgcgtctgca gcagatcgtc tcgaatctgc
     4621 tggtcaacgc cgtgaagtac acgcccgacg gcgggcagat ttcgattcgc gccagccgcg
     4681 aggggaacca ggtcgccatt gcggtcaagg acaatggaat cggcctttcg ccgcagatgc
     4741 aaacgcaagt gttcgacctc ttcactcagg ccgagcaggg aatcgagcgg gcccaggggg
     4801 gattgggcat cggcctgacg ctggtccgcc gcctggtcga gcggcatggt ggcagcgttt
     4861 cggtgaccag cgacggcccc caacggggca gcgagttcac ggtccgcctg ccggccatcg
     4921 agccgccgcc ggcggcggcc gcgcgcgaat ctcctacggg agcggaacct gcgcggtcgc
     4981 accgcatcct gattgtggac gacaaccgcg acgcgggccg cacgctgtcg ctgctgctca
     5041 aaaccctcgg ccacgaaacc gcgctggcat tcgacggccg gcaggcgctc gaagtcgcgg
     5101 ccgagttccg ccccgccgtc gcgctgctcg atctcggctt gccagaaatc aacggcttcg
     5161 atctcgcggt gcacctcaag caaggcgatc cgcgaatcca actgatcgcc atttccggct
     5221 acggccaggc cgccgaccgc cgccgctcac aggatgccgg cttcgaccgg catttcgtga
     5281 agccggtgaa gatcgacgag ctgctgagcg cgctgaatgc ggtcgagccg cagtagcgtt
     5341 atttctgggc cgccccgcga actacgtcgt cccagctagc gccgctgctg gaaccggaaa
     5401 ctgggcagag aggctgctga cttcgccgcg aatgcggtgg tgcagggcgg ggtcggtcgg
     5461 agcttggagc gcttgcagca tccagcggcc gatctgctgc atttcgctcg ggcccatgcc
     5521 gcgagtggta agcgcgggcg tgccgatgcg gatgccgctg ggatcgaggg gcttgcgggt
     5581 gtcgaacggg atcatgttca tgttgacggt gatgccgcag tgctcgagcg cctgcgtcgc
     5641 ctgcttgccg ccgatcgaga gcggcgtgac atcgaccagc atcaggtggt tgtcggtgcc
     5701 gccgctgacg agccgaaggc cgccgctcaa gaggacttcc gcgagcgcct gggcgttggc
     5761 gatgatcgcg cggccgtagt cctggaactc cggccgcaga gcttcgccga agcagacggc
     5821 cttgccggcg atgacgtgca tcagcggccc gccctgcaag ccggggaaga tcgtcttgtc
     5881 gatcgcctgg gcaaactcgg ccttgcagag cacggtgccg gctcgcggcc cgcggagcgt
     5941 cttatgcgtc gtcgtcgtga cgaaatcggc gaccggcact ggattgttgt gcaggcccgc
     6001 ggcgacaagg cccgcgtagt gggccatatc gaccatcagc ttcgcgccga cttcgcgggc
     6061 gatctcggcg aatttctcgt gcgggatctc gcggggatag gcgctcgcgc cggcgacgat
     6121 cagcttgggc ttgtgctcgc gggcctgccg ggcgacctga tcgaagtcga tccggtgcgc
     6181 gtccttggtc acgccgtagc tgtggaactt gtagagcttg cccgacaggt tcagcttcat
     6241 cccgtgcgtc aggtgccctc cgtgggccag gtccagcccc agcaccgtgt cgcccggctc
     6301 cagcaccgag aaatagaccg cctggttcgc ctgcgagccg ctgtgcggct gcacgttggc
     6361 gtgctcggcc ccgaacaatc gcttcacccg gtcgcgagcg aggttttcga tcacgtcgac
     6421 gtgctcgcag ccgccgtagt atcgccggcc gggatagccc tcggcatact tgttcgtgag
     6481 cacgctgccg acggcctgca ttaccgcggg gctggtgtag ttttcgctgg cgatcatctc
     6541 cagcccgtcc tgctggcgct gctcttcggc ggcgatcgcg gcaaatacgt ccgggtcttg
     6601 ctgctgcaaa atgttcatag cccgattgtc aaagcgggtc gcgatttcgt caattggccg
     6661 gagtgtcgtc cgctcgctcc gcgagcggac gacctcacgc ggagcgtgag gaatacacag
     6721 tttcagctat gatgcggcca tgcgcgtgat tccggtcatc gacctgatga atggcgtcgt
     6781 cgtccgcggc gtggcgggcc ggcgccagga gtatcggccg atccgcagca agcttgtggc
     6841 cgattcgcag ccgacggcgg ccgcccgggc ctttgccgag cattttggct tcgatacggc
     6901 ctacgtggcc gatctcgacg cgattcagtt tcggggggac gcgccgccct atttcaccgc
     6961 ctgctacgaa cggattgcag cctgcgggct ggctctgtgg ctcgacgccg gcgtcggcac
     7021 ggcggcagct gcgcagcatc tgctcgcggc ggtggaaccg ctggcgatgg acgttcaatt
     7081 cgtcatcggc ctggagtcgc ttgtgtcgca tttggagctt gcccagatcg tcgctgtgct
     7141 cggccgccag cggacaatgt tctgcatcga ccttgaggaa ggaaatctcc gcacgcaaat
     7201 tggccgctgg gccggcgccg atgccgtgga aatcggcttc ctggcgatgg cgcacggcct
     7261 gtcgcggctg atcgtgctgg acctggccga cgtgggcatg aacggcggca cgcggacgct
     7321 cgaattgtgc cgccgcattc gccacacttt tggctccgcc gtcgaactga ccgccggcgg
     7381 cggcgtgcgg ggcggcaagg acttgctggc cttggaagac gccggctgcg acgcggcgct
     7441 ggtggcctcg gccctgcacg acggccggct gacgcgggcc gagttggaca gcctggcgtg
     7501 ccggaaatga gggttgtgcg ggcgccgagc ggaattcgca gcgaattcag cgagcgacct
     7561 agacttgcgc tccataggaa gatctgggag cgctggcatg aacacctgga acacgtttca
     7621 cgacgaggcc tacacgtttc cgaatctctg cgccaagtgc gcggcgccgg atccaaccga
     7681 gacctggcag ctcaagtact atgaggtcga gtacccagaa ggaatgccgt ggggccccta
     7741 cgaccagaag ccgcgcaact acaacaccta tctgaccgac gtgcccctgt gcaaggaatg
     7801 cctcggcagc gtgcgctggc ggagccgtta ttgctggctc gcgggcgggc tggccggcgt
     7861 ggtcgcgggc atcgcgatgt tcggcctgat tagtgctcac cggccggctg agccggactt
     7921 gggtttcgtg cctttcgggg ccggcctctt cgtggccctg gccgtgggct acgtgctgaa
     7981 ggcggtgttc gtcgatgcac tttgctttgc caaattcaac ggccgggagc agaagctcgc
     8041 gttcaggaac aaggagttcc aggcccagtt cgaccggctg aattttcagt attcggccac
     8101 gacggggaaa tatgccgacg gcgggcgggc ctggtgatcc gcgaggcgcg acgacggcgc
     8161 agcgcactcg ctcttcggcc aggccggcct ggactggttc ctcaccggca gccgcgaccg
     8221 cttgatcgac cgggccagcg gcgagttcgt caactagcgg ccgcctgata caatcggcct
     8281 catgttcagc ggcccggaaa tcgccgccac gctcgccgag ctcgatttca cgcacgtcgt
     8341 ctggattccc gattcggcga ccggcccctg ggaaaccgcg ctcgcggcag cgcctgccct
     8401 gcggctggtg cgcgtctgcc gggaaggcga ggcctggccg ctggcggccg gcttgcacct
     8461 cggcggccag caaccgctgg tcgtcctgca gaccaccggc ctcttcgaat cgggcgacgc
     8521 gctgcggaac gtgctgctcg acatgcggct gccgctgtac gcgctgatcg gcgtccgcag
     8581 cgagctggtc gccgactcgc gggattcggc caggacgttc gccgagccga tcctgcgggc
     8641 ctggggcctc gattgcgtct ggctccgcgc agctgccgac cggacgaaat tcgtagagca
     8701 tgtccggcgc tgccgccagg agcaaaagcc tggcgcggcg ctcctggccg aaggaccgcc
     8761 gtgatgcagc gcaagcccca ttcgggaatg cccgtcgccg acgcggtgca ggtgctcatc
     8821 gaccaccgcg cgagtgggca gatcgtcgtc acgaaccagg gcgccgcgcg cctgtggccc
     8881 aagctcagcc ggcggccgct cgacctgcac tacaacccct cgacgatggg cggcgcgatt
     8941 ccgctggcgc tcggcctggc cctggcccag acgcagcggg aagtgctggt ggtctcgggc
     9001 gacggcgcgc tgctgatgag cctgggcgcg ctggttaccg tcagtggcag cggcgcggcg
     9061 aacctgaccg tagtcgtgct cgacaacggc ctgtacgaag tgactggcgg gcagaaaact
     9121 ccagcgacgg ccgccgtcga tttcgccggc ctggccaaga gcgcaggatt tcccagcgcc
     9181 taccggttcc gcgatctggc cgtctggcgc gggcaggccg ccgccgcgct atccgcggcc
     9241 ggcccgcggt tcatctcgct cgaagtcgaa ccgatgccgc cggaatacct gcgcagtccc
     9301 acgccgccgc tggccgaaca aatcgtcgag tttcgccgcg aggtgggcgg gcgcgtggag
     9361 attagcagcg tccagtcctg ctgccgcgct ggatcatgat ttggtttgag gaggcaaatc
     9421 actgccgatt tgcacggaaa cggagaattg acgcttatct ctgtccaacc atgtgatcgt
     9481 gtagacaatc gggatggcgg atggtttcgt gtgagctatg cacagtccga ctcggcgctg
     9541 agcagtcgca tcgaaactga tttgacgatg tccatcgatc gccgatatgg agggcgcgat
     9601 attcaagtcg cgaaccagct tgttgaccag ctgtaccacc tgaggagagt cgcaccccgg
     9661 gagttgtggc ccggccaaca gttgaccgtc gtcgatggat tgtcgtattg cgccgatcat
     9721 cgcaggccag acctgattga tctcatagcc caagaatccg ctgaagagga gtgtgaagca
     9781 taggcacccg atccaagccg ctcgtgcgac attggtccgg agcaatggct cgaaaatcgt
     9841 tgctggcgcc gagccggttc gcatcaatcg caaggagcga agcgccgaac gggccagaaa
     9901 aagcgccacg gccagcatgg cccactggat gggatcgaac agcaatagca gtgcgaacag
     9961 cgagtcccaa tgccagagcg ctacgaaggc cacgatcagc ggtcgagcta atagcgcgac
    10021 a
//
LOCUS       DASRJB010000219         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2601143, whole genome shotgun sequence.
ACCESSION   DASRJB010000219 DASRJB010000000
VERSION     DASRJB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601143"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VFB80_06085"
     CDS             complement(<1..1006)
                     /locus_tag="VFB80_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZN33367.1"
                     /translation="MRCFFALALAGCLLAAHAAAAQDSPASRVYQNRLTRIEEPRPIL
                     ADHPEFFEPILEQAHFEAPPVVVDQRADLHVRAWRFSYNARGIIEMPNHLKAAETAVI
                     MVHPWGIDDGQGWNTPEPAGVADFCTKEKNHLAGRHTREIVRPFINSLRGKVGLVLYS
                     LPGDEDPIRRKLYRSFQHQPTDGERREGANELAAKLQSFQYEGQPLPPRLELSADKPV
                     IDYFRQFPGLDAGPKFNNEGFWKLPIPVTTDVDVHASDVTIYDAQGYEPLKKFLLSHG
                     IRHVLLTGYATDMCFCRTTAGYENLARDFNVFLVGDASLATFPANRSPRFAVNATISL
                     A"
     gene            1186..2742
                     /locus_tag="VFB80_06090"
     CDS             1186..2742
                     /locus_tag="VFB80_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HZN33368.1"
                     /translation="MCSTYARLAAALIALGLTATAAADTLDEIQKRGELIFGGDAEGG
                     GPFVFPREDDPTQVQGFEIELASLLAAKLGVKARFSQGQWEKLPDLLERGDIDIVLNG
                     YEWTPTWSQRYAASIPYYVYELQMLVRKNDDRLKQLKDLSMPGLDGKMRIAVLGGSAA
                     ETYVRKTFGSDVEVVTYEGTTDAMRGVELAIDGVDATMQDLPIATYYESRFPLLRRLG
                     EPVEPGFYVALARKSDQRLVAALNRAIVDCYLDGSFPRVLARYGLWNETQRRRGLVTD
                     SQGNFQPDPPADGAAAVPPLTAGAGNHIFTRRVPLLIQAAVITVLLASLAMPLAILVG
                     LSIAVGRLYGPKFLRPPLGWYVEIVRGTPLVLQLYLIFFLLPEWGLSIPAFWAAIIGL
                     AVNYSAYEAEIYRAGLQAIPRGQMEAALSLGMPKWLALRRIIIPQAFRIVIPPVMNDF
                     IAMFKDTAVCSVITVVELSKEYYIHARSTGAVVELGLVTALLYLVMSYPLSIFVGRME
                     RRLGEGRHHD"
     gene            2735..3472
                     /locus_tag="VFB80_06095"
     CDS             2735..3472
                     /locus_tag="VFB80_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZN33369.1"
                     /translation="MIEISNVHKRYGSTSVLCGVSIEVKKGEVSVLLGPSGGGKSTLL
                     RTINALETFDEGRIRIDDLELTANGRQEAHIRQIRRRVGMVFQQFNLFPHLSVLQNVT
                     EAPRHVLGTSRDEAETLAKELLTRVGLAEKLHARPTTLSGGQQQRVAIARALAMKPEA
                     ILFDEPTSALDPRMTAEVVSVVADLAKTGQTMIIVTHALGFARRVAHTVHVMHAGRVA
                     ESGPPAQVFDAPEQEVTRTFLNQARSD"
     gene            complement(3486..>4113)
                     /locus_tag="VFB80_06100"
     CDS             complement(3486..>4113)
                     /locus_tag="VFB80_06100"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZN33370.1"
                     /translation="SDNGPAITPAHPYGSAGPLRNKKGSVYEGGIRVPGIIRWPGKTT
                     AGSTSDEPVCGVDFLPTVCTITGIAPPQDRKLDGASLLPILAGRKLERTTPLYWHFNH
                     AFGGPLVALRSGDWKILATLDKEPLRGTAITEQSEADFHAAELKEFQLYNLKDDIGET
                     NDLAAAQPVKLKEMQSLLSAKYREVRDESPTWPAWEPPPAPAKAKKKQ"
BASE COUNT          670 a         1312 c         1389 g          742 t
ORIGIN      
        1 ccgcgagcga gatggtggcg ttgacggcaa accgcggtga gcggttggcg ggaaaggtcg
       61 ccagggaggc gtcgcccacg aggaatacgt tgaagtcgcg agccaggttt tcgtaaccag
      121 ctgtcgtgcg gcaaaagcac atgtcggtcg catagccggt gagcagcacg tggcgaatgc
      181 cgtggcttag cagaaacttc ttcagcggct cataaccctg ggcgtcgtag atagtcacgt
      241 cgctggcgtg cacgtccacg tcggtggtga ccgggatcgg cagcttccag aagccttcat
      301 tgttgaactt cggcccggca tccaggcccg gaaactgccg gaagtagtcg atcaccggct
      361 tgtcggccga cagctccagc cgcggcggca gcggctgccc ctcgtactgg aacgactgca
      421 gtttggccgc cagctcgttt gccccctcgc gccgctcgcc gtcggtcggc tgatgctgga
      481 acgaccggta gagcttgcgg cggatcgggt cttcgtcgcc gggcaggctg tagaggacca
      541 ggccgacttt gccgcgaagc gaattgatga acggccggac gatctcgcgg gtatggcggc
      601 cggcgaggtg gttcttctcc ttcgtgcaga agtcggcgac gcccgccggc tcgggcgtgt
      661 tccagccctg gccgtcgtcg atgccccagg gatgcaccat gatgacggcc gtctcggcgg
      721 ccttcagatg gttgggcatc tcgatgatgc cgcgggcgtt gtacgagaac cgccaggccc
      781 gcacgtgcag gtcggccctc tggtccacga ccaccggcgg cgcctcgaaa tgggcctgtt
      841 ccaggatcgg ctcaaaaaac tctggatgat cggccagaat cggccgcggc tcttcgatcc
      901 gcgtcagccg gttctggtaa acgcgacttg ccggggagtc ctgggccgcg gccgcatgag
      961 cggcaagcag gcagccggct aaagcgaggg cgaagaaaca gcgcatctcc agtctcctgg
     1021 aaaattggtg tcggactgag cggtcgattt gtggatatta ctctttatga cgggcaggga
     1081 accatggaat acacagacga cagggaagat aggagatacc tttcgtgtat tccgtgtatt
     1141 tcgtggttcc tgctttctgc cgcagtgacc ttcggaggct tctgaatgtg tagcacctac
     1201 gctcggctgg ccgcggcctt gatcgctctt gggctgactg cgaccgccgc ggccgacacg
     1261 ctcgacgaga ttcagaagcg cggcgagctg atttttggcg gcgacgccga aggcggcggg
     1321 cccttcgtct tcccgcgcga ggacgacccg actcaggtgc aaggttttga gatcgagctg
     1381 gccagcctgc tggccgcgaa gctgggcgtg aaggcccgct tctcgcaggg gcaatgggaa
     1441 aagctgcccg accttttgga gcggggcgat atcgacatcg tcctcaacgg ctacgaatgg
     1501 acgcccacct ggagccagcg gtacgccgcc tcgattccgt attacgtcta tgagctgcaa
     1561 atgctcgtgc ggaagaacga cgaccgcctg aagcagctca aggacctcag catgccgggc
     1621 ctcgacggca agatgcgaat cgcggtgctc ggcggttcgg cggccgagac ctacgtgcga
     1681 aagacgtttg gcagcgacgt ggaggtcgtg acctacgagg gcacaaccga cgcgatgcgg
     1741 ggcgtcgagc tggccatcga cggcgtcgat gccaccatgc aggacctgcc gatcgccacg
     1801 tactacgaat cgcggttccc gctgctgcgg cggctggggg agccggtcga gccggggttc
     1861 tatgtcgcct tggcccgcaa gagcgaccag cggctggtcg cggcccttaa ccgggcgatc
     1921 gtcgattgct atctcgacgg cagctttccg cgggtgctgg ctcggtacgg cctgtggaac
     1981 gagactcagc ggcgacgcgg cctggtcacc gattcccaag gaaatttcca gcctgatccg
     2041 cccgcggacg gcgccgccgc tgtcccgccg ctgaccgccg gggcgggcaa tcacatcttc
     2101 acgcggcgcg tgccgctgct cattcaagcg gcagtcatca cggtgctcct ggcctcgctg
     2161 gcgatgccgc tggcgatcct ggtgggcctt tcgattgcgg tcggccggct gtacggcccc
     2221 aagttcctgc ggccgccgct gggctggtac gtcgagatcg tccgcgggac gccgctagtg
     2281 cttcagctct acttgatctt cttcctactg ccggagtggg gcctgtcgat tcccgcgttt
     2341 tgggcggcga tcatcggact ggcggtcaac tactcggcct acgaggcaga aatctatcgg
     2401 gccggcctgc aggcgattcc gcgcgggcag atggaggcgg ccctttcgct gggcatgccc
     2461 aagtggctcg cgctgcggcg gatcatcatc ccgcaggcgt ttcgaatcgt cattccgccg
     2521 gtcatgaacg acttcatcgc catgttcaag gacacggcgg tctgctcggt gatcacggtc
     2581 gtcgagttga gcaaggagta ctacattcac gcccgcagca ccggggccgt cgtcgagctg
     2641 ggcctcgtca ccgccctgct ctacctggtg atgagctacc cgctgtcgat ctttgtcggc
     2701 cggatggagc gccggctggg ggagggccgc caccatgatt gagatcagca acgtccacaa
     2761 gcggtacggc agcacgagcg tcctgtgcgg cgtttcgatc gaggtcaaaa aaggggaggt
     2821 cagcgtgctg ctgggcccct cgggcggcgg caagagcacg ctgctgcgga cgatcaacgc
     2881 cctggagacc tttgacgagg ggcggatccg catcgacgac ttggagctga ccgcgaacgg
     2941 gcggcaggag gcccacatcc ggcagattcg ccgccgcgtc ggcatggtct ttcagcagtt
     3001 caacttgttc ccgcacctga gcgtcttgca aaacgtcacc gaagcgccgc ggcatgtgct
     3061 cggcacgtcc cgcgacgagg ccgagacgct ggccaaggag ctgctcaccc gcgtcggcct
     3121 ggcggagaag ctgcatgccc gccccacgac gctctcgggc ggccagcagc agcgggtggc
     3181 catcgcccgc gccctggcga tgaagcccga ggcgatcctg tttgacgagc cgaccagcgc
     3241 cctcgacccg cggatgaccg ccgaggtcgt cagcgtcgtg gccgacctcg ccaaaaccgg
     3301 ccagacgatg atcatcgtca cgcacgccct gggcttcgcc cgccgcgtcg cccacacagt
     3361 gcacgtcatg cacgccggcc gcgtcgccga atccggcccg ccagcacaag tcttcgacgc
     3421 tcccgagcag gaagtcaccc gcaccttcct gaaccaggcc cgcagcgact agctctagcc
     3481 gcgcgtcatt gctttttctt cgccttggcg ggggccggcg gcggctccca ggcgggccag
     3541 gtgggcgatt cgtcgcggac ttcgcggtat ttcgcagaca gcaaggactg catctctttg
     3601 agcttcaccg gctgcgcggc ggccaggtcg ttcgtctcgc cgatgtcgtc cttcaggttg
     3661 tagagctgaa actccttcag ctctgccgcg tggaaatcgg cctcggattg ctcggtgatc
     3721 gcggtgccgc gtaacggctc cttgtcgagc gtcgccagga tcttccagtc gcccgatcgc
     3781 agggcgacca gcgggccgcc gaaggcgtga ttgaagtgcc agtagagggg cgtggtccgc
     3841 tcgagtttgc gcccggccag gattggcagc aggctcgcgc cgtcgagctt gcggtcctgc
     3901 ggcggcgcaa tgccggtgat ggtgcaaacg gtgggcaaaa agtccacgcc gcagaccggc
     3961 tcgtcgctgg tgctgccggc agtggttttg cccggccagc ggatgatccc cggcacgcgg
     4021 atgccgccct catagaccga ccccttcttg ttccgcagcg gcccggccga gccgtaggga
     4081 tgcgcgggcg tgatcgccgg gccgttgtcg ctg
//
LOCUS       DASRJB010000220         6627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4013236, whole genome shotgun sequence.
ACCESSION   DASRJB010000220 DASRJB010000000
VERSION     DASRJB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013236"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3319
                     /locus_tag="VFB80_06105"
     CDS             <1..3319
                     /locus_tag="VFB80_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33371.1"
                     /translation="LRRLNRRAEAEREYRTALQLKPDYAEAHLNLGTVLFDEGLLEEA
                     EAEFLEALALRKNYAKALANLGNVYQSQDRLIEAEFTCRLAVAIDRDDLFAQLTCGDV
                     LLKRDMYADAEAAYREAVRLDGRVGRHHQALATALRLQGKLDEAAAEFSTAILLDPRL
                     DQSRSGLQSLVGARRTPVEAEAMFRSAVERDPRNANLRMQLAEALASQGKDAAAAAEY
                     REIARLQPANGYARAKMSSLLTRLGKIEELDAAYQELVRARPDEPAIHQEYGYFLSNQ
                     GRPSDAERILRAGVARIPSDVGLRLQFAKVLLASGKSADALPELEAAIAARPESGTLH
                     VEYALALTQVGRATEALAEYREGVRLTPASSQYWMHLADALAKSGPATEAEAAYRDSL
                     RLDAKNLYCRHSYASFLIAQQRLLDAETELRENLRRAPHYLMSRQSLIDVLRKQGKLA
                     ELEPVYRDGVAQQPQEFLRYRELSDYLLSQTRPADAEQVLRDAVAVMPESVAARDHLA
                     KFLDSQGQRPGAEAELRQVLRLNPDDPTLHFRLASLLRQRGQLQDAAAEFREAVRLNP
                     HEPQYSNTLADVLKDLGQPDEAVKVLRELVERRPEDQESLLRLARLLVQLKKREDAEA
                     AFRSAIRLRPMFVGPYFELGGFLHGQGQLEAAEAAFRDLLRIAPGDGNGHERLAGVLI
                     SRGQFTEAEGELRQALSLNPESTGVHWGLGNCFQAQGRTDEALAAFQEAIRRQPAEAY
                     PHHQVGKLLLKQGRFQEAEVQLAEAANLRPLDQTIKNDLAAARTKVKLLADEPALRAA
                     VSEKPDDHQARQRLGNLLIALGKFADAEAELRHAVRLAPTQAPYHTNLAQALIQQGKL
                     AEALGEYREAARLQPQVAAHYYAVGNFGGRLAMWNDSAAALLKVTELDPKNHLAWYGL
                     AALRLEMGDVEGYRQASRDMLTRFEGAAQPEIWERTAKTCLLTADGAGELKRALALAD
                     RAVTGTEKNRYYWYIQFTKGLSEYRAGRYAESLPWLEKFTGRPQAAGPAEATYAPYYT
                     AGHAVIALTQHRLGEGEKAAAELAAAQNLMVRWMPDGSAARPFELNWDDWLRARMLVR
                     EAESALQAAAAEAPQDK"
     gene            3561..4043
                     /locus_tag="VFB80_06110"
     CDS             3561..4043
                     /locus_tag="VFB80_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33372.1"
                     /translation="MRRTLNCVVSAAFVLAGWTASAQAQTVPLFEHCEGTVQSFNPLT
                     GVLTFAGKGRATVLGLYAVEGSSAAFPLSPTDGLVSGGDTSTTRDGATIKSEFEGVYE
                     VLESGKIKFRVTARWGEGTGRLAGVTGEAEVVAVLDGLAPGSAFAYDTVGTLTFPHQP
                     "
     gene            4085..4894
                     /locus_tag="VFB80_06115"
     CDS             4085..4894
                     /locus_tag="VFB80_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33373.1"
                     /translation="MATRSLKRGFTLVEILVVIAIVAILAGLLLPAVQMAREAARRMR
                     CQNNLHQIGLALLGFESLHGRLPAGRDAERSRQHAWSTAILPQLEQAALYQRYDFSKS
                     WKDPSNQQVTAANVAVYRCPSAVGKWDGKIDYGGNYGSALTGLKPGFSSGCAWEAGAF
                     PPVHLAMPGQYRSAAVRLGEITDGTSQTFLVLEDADRPAAQGGLWASGHNCFAHDGPI
                     NANVSKEIFSRHPCGANALLADGSVRLLTDSIALPLLGALCTRAGGEAVAP"
     gene            4953..5468
                     /locus_tag="VFB80_06120"
     CDS             4953..5468
                     /locus_tag="VFB80_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33374.1"
                     /translation="MRTYILSTVILALAATPAFAIIGGGHGNGGGGPDTKYAFLNVVN
                     GSDTGVEVSIDGGAFVALEPGEGAPATYVASKKLEATIIARLLSAPGVSSTATAQLQA
                     NKTTVATVSASSSAVSIAVARPGNVARHIREAGVALASTGGLLPLLWLGLLLGRRPQR
                     RQRPEACNLVA"
     gene            complement(5474..>6627)
                     /gene="lnt"
                     /locus_tag="VFB80_06125"
     CDS             complement(5474..>6627)
                     /gene="lnt"
                     /locus_tag="VFB80_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN33375.1"
                     /translation="LAHSQLGWLAFCQVAEFTGAYGVSFLLVWGAAVAAEVWHRRRQA
                     LPVAGVCALIVLANVGWGYWRIGRVEAASAARPKLTVLLVQPGGRGEEMPDRLRRMSL
                     AQCERADLVVWGEGSIGYFADDLASFHDVEQVRRAARFESPDPRPCTGLQRPLLCGGG
                     SFAPGAAAKGPYCNTAHLIAADERIVGRYHKRVLMPWGEYAVGQQWLPGLHGLLGEVD
                     PWVAGNSAAPLDLNPQARLGVLICYEDLLPQSARETVQSGANVLINLNNLQTWGGTIA
                     PLQHQLLARFRTIENRRWLVRCGTVGSTAVLSATGRVERQAPLSQPATLVASVPLLES
                     RTVYTRYGDVFAMVCAVIGAALIVRQAAAHWWPRKATASTVPSVEAAEA"
BASE COUNT         1162 a         2196 c         2192 g         1077 t
ORIGIN      
        1 gctgcggcga ctgaatcggc gggcggaggc agagcgggaa tatcggacgg ccctgcagct
       61 gaagccggac tatgccgaag cccatctgaa cctgggcacg gtgcttttcg acgagggact
      121 tctagaagag gccgaagcgg agtttctgga agcgctcgcg ctgcgaaaga actatgccaa
      181 ggccttggca aatctgggaa acgtttacca gtcgcaagat cgtctgatcg aagccgagtt
      241 cacttgccgc ctggccgtcg ccatcgatcg cgacgatttg tttgctcaac tgacctgcgg
      301 cgacgtgctg ctgaagcgcg acatgtatgc ggacgcagag gctgcctatc gcgaggccgt
      361 ccgcctcgat ggccgcgtgg gccggcacca ccaggctctg gccaccgccc tgcggctgca
      421 aggcaagctg gacgaggccg ccgcagagtt tagcacagcg attcttttgg acccgcggtt
      481 ggaccaaagc cgatccgggt tgcagtccct cgtcggggcc aggcgcacac cggtcgaagc
      541 cgaggcgatg tttcggtcgg cagtcgagcg cgatccgcgg aacgcgaacc tgcgaatgca
      601 gctcgccgaa gcgttggcct cgcaggggaa ggacgccgcg gcagcagcgg aataccgcga
      661 gatagctcgc ctccagccag ccaatggcta cgcccgcgcg aaaatgtcca gcctgctgac
      721 tcggctggga aagatcgagg aactggatgc ggcctaccag gagttggtcc gcgcgcggcc
      781 ggatgaaccc gcaatccacc aggagtacgg ctacttcctg tcgaaccagg ggcggccgtc
      841 ggacgccgag cgcatcctgc gggccggcgt cgcgcgaatt ccatcggacg tgggcttgcg
      901 gttgcagttc gccaaagtcc tcttggcgtc gggtaagtcg gcggatgcgc taccggagct
      961 tgaagcggca atcgccgcgc ggcctgaatc gggcacgctg catgtcgaat acgccctcgc
     1021 gctgacgcag gtcggccggg ccacggaggc gcttgccgag tatcgcgaag gtgttcgttt
     1081 gaccccggcc agcagccagt attggatgca cctggctgac gccttggcga aatccgggcc
     1141 tgcgactgaa gctgaggccg cctatcgcga cagccttcgg ctggacgcca agaatctgta
     1201 ctgccgacac agttatgcgt ccttcctcat cgctcagcag cggctcttgg acgcggagac
     1261 ggaactgcga gagaacctgc ggcgagcgcc acattacttg atgagccggc agagcctgat
     1321 cgacgtcctg cggaaacagg gcaagttggc ggagttggag ccggtctatc gtgacggcgt
     1381 cgcccaacaa cctcaggaat tcctgcgtta cagagagttg tccgattatc tgctgagcca
     1441 gacccggccg gcggatgccg agcaagtcct gcgcgacgcc gtcgccgtga tgcccgaatc
     1501 ggtcgcggcg cgcgaccact tggcaaagtt cctggacagc cagggacagc gcccaggtgc
     1561 ggaagccgag ctgcgccaag ttctgcgact caatcccgac gatccgacgc tccatttccg
     1621 gttggcgagc ctcctgcggc agcgcggcca gcttcaggac gcagccgcgg aattccgcga
     1681 ggccgtacgc ctgaacccgc atgaaccgca gtattccaac acgctagcgg acgtgctcaa
     1741 ggacctggga caacccgacg aggcggtaaa ggtgctgcgt gagctggttg agcggcggcc
     1801 ggaagatcag gagtccttgt tgcgactggc gcggttgcta gtgcagctta agaagcggga
     1861 agacgccgag gccgcgtttc ggtcggcgat ccgtctgcgt cccatgttcg tcggaccgta
     1921 tttcgagctg ggcggttttc tgcatggcca aggacagctc gaggctgctg aagcagcctt
     1981 tcgcgatctg ttgcggatcg ccccaggcga tggcaatggt cacgagcggt tggcgggcgt
     2041 actgatcagt cggggccagt tcaccgaggc cgagggcgag ttgcgccagg ccttgtcgct
     2101 gaaccccgag tcaaccgggg tccactgggg tctgggcaat tgctttcagg cccaagggcg
     2161 aactgatgag gccctggccg ccttccagga ggcgattcgc aggcagcctg cggaagccta
     2221 cccgcatcat caggttggca aattattgct caagcagggg cggtttcagg aggccgaagt
     2281 gcaattggcg gaggcggcaa acctgcgccc tctggatcaa acgatcaaga acgacctcgc
     2341 cgcagcccgc accaaggtga agctgctcgc cgacgagcct gccctgcggg ccgccgtcag
     2401 cgagaagccc gacgaccacc aggcccgcca gcgacttgga aatctcctga ttgcgctggg
     2461 caagttcgcc gacgccgagg ccgagctgcg gcacgctgtc cgcctcgcgc cgacacaagc
     2521 cccctaccac acgaacttag cccaagcgtt gatccagcaa ggcaagctgg ccgaagcgct
     2581 cggcgagtac cgcgaggccg cgcggcttca gccgcaggtg gctgctcact attatgcggt
     2641 gggaaatttt ggtggccgcc tggccatgtg gaacgactcg gccgcagctc tgctcaaggt
     2701 cactgagctg gatccgaaga atcacctcgc ctggtatggc ctggcggcgt tgcgattgga
     2761 aatgggcgat gtcgagggtt atcgccaggc cagccgcgac atgctcacgc gattcgaagg
     2821 ggcggctcag ccggagatct gggagcggac cgccaagacc tgcctgctga cggctgacgg
     2881 cgcgggagag ctgaagcggg cgctagccct ggccgaccgc gcagtaaccg gcacagaaaa
     2941 gaaccgttac tactggtaca tccagttcac gaaaggcctt tcggaatacc gggcgggccg
     3001 atacgcggaa agtctgccct ggctggaaaa gtttaccggg cgtccgcagg cggcaggtcc
     3061 ggcggaggcg acttacgccc cctactacac ggccgggcac gcggtcatcg ccttgacgca
     3121 gcaccgctta ggcgagggcg agaaagccgc cgcggagttg gccgccgccc agaatctgat
     3181 ggtcaggtgg atgcccgacg gctccgcggc acggccgttt gagctcaact gggacgattg
     3241 gctgcgcgcg cgaatgctcg tgcgcgaggc cgagagcgcg ctgcaggcgg cagcggcaga
     3301 agcgccccaa gacaagtagt ctcccgccgc ctctcttcgc ctgcgctaag cgcttcgttc
     3361 tcccgccgcc gcatggcttt ttggcgatct gctggggtga ttcctggccg gatttcgcat
     3421 tgttagcgga tcaacagaac gggccggcaa gtactcgccg cgaagcacag ctacttgccg
     3481 gcccagccag atcgggcgct ggtctcgcgc gcccggcctg tctcgcttca ttctaacgcg
     3541 ggccaagaag ggactgcatc atgagacgta ccttgaattg tgtggtttcg gcggccttcg
     3601 tgctggcagg ctggactgcc tcggcacaag cgcagacggt gccacttttt gagcactgcg
     3661 aaggcacggt gcagtccttc aacccgctga ccggcgtgct gacgttcgcc ggcaagggcc
     3721 gggccacggt tttgggcctg tacgcggtcg aaggcagcag cgctgcgttc ccgctgtcgc
     3781 ccaccgacgg tctggtcagc ggcggtgaca cgagcacgac ccgcgacggc gcgacgatca
     3841 agagcgaatt cgaaggcgtc tatgaagtgc tggagtcagg caagatcaag ttccgcgtga
     3901 ctgcccgctg gggtgagggc acgggccgcc tggccggcgt caccggcgag gccgaggtcg
     3961 tggctgtgct cgacggcctg gcgcctggca gtgccttcgc ctacgacacg gtcggcacac
     4021 tgaccttccc gcaccaaccg taaccgccga gtcagcttct ccattgctct ttccggaggt
     4081 tgccatggcc actcgctcgc ttaagcgcgg attcacgctc gtcgagatcc tggtcgtgat
     4141 cgccatcgtg gcgattctgg ccggcctcct gcttcccgcc gtgcaaatgg cccgcgaggc
     4201 cgcccgccgg atgcggtgcc agaacaacct ccaccagatc ggcctcgccc tgctcggctt
     4261 cgagtcgctc cacggccgcc ttccggccgg gcgcgacgcc gagcgcagcc ggcaacacgc
     4321 ctggtccacg gcgatcctcc cgcagctgga gcaggcggcg ctctaccagc gctacgactt
     4381 cagcaagtcg tggaaggacc cgtccaatca gcaagttacg gcggccaatg tcgccgtcta
     4441 tcgctgcccg tcggccgtcg gcaagtggga cggcaagatc gactacggcg gcaactacgg
     4501 cagcgctctc accgggctca agccgggctt ttcttccggc tgcgcctggg aagcgggcgc
     4561 gtttccgccg gtccacctgg caatgccggg ccaatatcgc tccgccgcgg tgcggctggg
     4621 tgaaatcacc gacggcacgt cgcagacgtt cctggtcctg gaggacgccg accggccggc
     4681 ggcccagggc ggcctgtggg ccagcggcca caactgcttc gcccacgacg ggccgatcaa
     4741 cgccaacgtc tcgaaagaga tcttcagccg tcatccgtgc ggagccaacg cgctcctggc
     4801 ggatggcagc gtccgattgc ttaccgattc catcgcgctg ccgctgctgg gtgcactgtg
     4861 cacccgcgcc ggcggcgaag ccgtcgcgcc ttgaattccc atgcgtatgg gaaggggagc
     4921 gacggtcaga acctgtttct cggagaacca ccgtgcgtac ttacatcctt tcgacagtta
     4981 tcctcgccct ggccgccacg ccggcctttg cgatcattgg cggcggccac ggcaacggag
     5041 gcggcggccc cgacaccaag tatgccttcc tgaatgtcgt caacggcagc gacacgggtg
     5101 tggaggtcag catcgatggc ggcgccttcg tcgccctgga gccgggcgaa ggagccccgg
     5161 ctacctacgt tgcttcgaaa aagttggaag cgacgattat cgcgcgcctg ctgtcggcgc
     5221 ccggcgtcag cagcaccgcg acggcccagc tccaagccaa caagacgacg gtcgccaccg
     5281 tctcggcgtc cagctcggct gtgtcgatcg cggtcgccag gccaggcaac gtggccaggc
     5341 acatccgcga agccggcgtg gcgctggcct cgaccggcgg cctgttgccg ctgctctggc
     5401 tgggcctgct cctcggccgc cggccgcagc gccgccagcg acctgaggcc tgcaaccttg
     5461 ttgcctaagg cggtcatgcc tccgcagcct ccaccgacgg aacggtggag gctgttgcct
     5521 ttcttggcca ccagtgggcc gcggcttgcc ggacgatgag tgctgcaccg atcactgcgc
     5581 agaccatggc aaagacgtcg ccgtagcgcg tatagacggt ccgcgattcg agcaacggga
     5641 cagacgcgac cagcgtggcg ggctggctca gcggtgcctg tcgctcgacg cggcccgtcg
     5701 ccgagagcac cgcggtgctg ccgactgtgc cgcatcgcac cagccagcga cggttttcga
     5761 tcgtgcggaa ccgggccagc agttgatgct gcaggggcgc gatcgttccg ccccacgtct
     5821 ggaggttgtt caggttgatc agcacgttgg cccccgactg caccgtctcc cgcgcgcttt
     5881 gcggcagcaa gtcctcgtag cagatcagca ctcccagccg ggcctgcgga ttcaggtcca
     5941 gcggcgcggc cgagttgccg gccacccagg gatcgacttc gcccagcagg ccgtgcaggc
     6001 ccggcagcca ctgctggccg acggcgtact cgccccacgg catgagcacc cgcttgtggt
     6061 agcggcccac gatccgctcg tcggcagcga tcaggtgagc cgtattgcag taaggcccct
     6121 tggcggctgc gcccggtgcg aagctgcctc cgccgcagag cagcgggcgc tgaagtccag
     6181 tgcagggacg aggatcgggc gattcaaatc gcgcggcccg gcgaacctgt tccacgtcat
     6241 gaaaactagc caggtcgtcg gcaaagtagc cgatcgaacc ttcgccccac acgaccagat
     6301 ccgcccgctc gcactgggcc agcgacatgc gccgcagccg gtccggcatt tcctcgccgc
     6361 ggccgccggg ctgaacgagc agcaccgtta gcttcggacg agcggcgctg gcggcttcga
     6421 cccggccgat cctccagtaa ccccaaccca cgttcgccag aacgatcagc gcgcaaacgc
     6481 ccgcgaccgg cagcgcctgc cgccgccgat gccagacttc agcggccacc gccgcgcccc
     6541 agacgagcag aaagctcacg ccgtaggccc ccgtaaactc ggcgacctgg caaaaggcca
     6601 gccagccgag ctgcgaatgc gccagcc
//
LOCUS       DASRJB010000221         4308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1756078, whole genome shotgun sequence.
ACCESSION   DASRJB010000221 DASRJB010000000
VERSION     DASRJB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756078"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..326)
                     /locus_tag="VFB80_06130"
     CDS             complement(123..326)
                     /locus_tag="VFB80_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33376.1"
                     /translation="MLQVIAGCVVRTLPNWSREVAVSCRVPLFKTVALAGLTEMLDSV
                     WTTVTVALLTAIWLSASRIATAN"
     gene            456..2060
                     /locus_tag="VFB80_06135"
     CDS             456..2060
                     /locus_tag="VFB80_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZN33377.1"
                     /translation="MPDTGNGLAAVYYNNINLTGTTLTRTDATINFDFATGSPGASIA
                     PDTFSARWIGQIEPRFSETYTFTTTSDDGVRLWVNNQLLIDQWNDHAVKQHSGTIALV
                     AGERPAIRLEYYENTGAAVIKLQWQSASQPREIVPQSRLYSGPLDPILTSIAVSPASA
                     NLAPGTTTQFTATALDQFGNALQAQPALAWSIDAGGIGVVSAAGLYQAPVTGSGSAIV
                     RASAGAIGGVATVTVGTAAAGAGNGLAAVYFNNSNLTGTSIVRTDQAVNFDFASGSPA
                     SFIAADTFSARWIGQIEPRFSETYTFTTTSDDGVRLWVNDRLLIDQWNNHSARQHSGT
                     IALVAGERPAIRLEYYESSGLAVIKLQWQSASQPREIVPKSRLYGGAPVRVNFQPAGA
                     PVPTGYVVDAGETFAARASGYSYGWNLDNTLSPRDRNSSLAPDQRYDTLTLLQTPENP
                     GAAWEIAVPTGAYRVHLVAGDAGSLAGVFKTSVEGVLIVDGTPTSGQRWLEGWRDILV
                     SDGRLTVTSAAGASGNKLNFLDIIPL"
     gene            2146..2697
                     /locus_tag="VFB80_06140"
     CDS             2146..2697
                     /locus_tag="VFB80_06140"
                     /inference="COORDINATES: protein motif:HMM:NF045184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33378.1"
                     /translation="MAATWKQLTDFLVEQGIEQIAHTQKNYLAHLISVYKLMEASGYG
                     EELCRAGLFHSIYGTEKFQGFKLPLTRRAELAEMIGSRAERLAYWNCQMDRASLDGLL
                     TQDQEPLLLRNRETGEEMPLTRRELDDLCCVHLFDWLEQAPRSRFGWDYRRQAYRQMA
                     ERLGGAALAAYDQVFAAAPAGAT"
     gene            2711..3826
                     /locus_tag="VFB80_06145"
     CDS             2711..3826
                     /locus_tag="VFB80_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN33379.1"
                     /translation="MTTPFLSANEVGTAGARTLGGEFYTSREIFERERERIFSRRWVC
                     VGREQQLAGGGYFLRDVAGESLILLRDQAGQVRAHYNVCRHRGSRLCEEASGRLAATI
                     QCPYHAWTYALDGRLLGAPGMEALAGFDKSAHGLAAAAAGVWEGFLFVSLAQQPEPLE
                     QAFAALAGKFARYELPRLASVRRIDYDVRANWKLIVQNYSECYHCPGVHPQLVKLSPA
                     DSGKNDLTSGPFLGGYMSLPAGAALTKSGRRCAIDVGPLTDEDRQRVYYYSIFPNLLL
                     SLHADYVMVHTLWPLAPDRTRVECEWLFSPDAAGRNGFDPDGGVQFWDETNRQDWHVC
                     ELSQQGVGSRAYRPGLYSPRESISAAFDREVLRALAE"
     gene            3982..>4308
                     /locus_tag="VFB80_06150"
     CDS             3982..>4308
                     /locus_tag="VFB80_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33380.1"
                     /translation="MKRTSSSAGWLCCAALVLFCCAARAQLPDRPISPAERQFWAFQQ
                     PRRVETPAASAAGWEQNAIDAFLLAAMKAKEVRPAPPAGRRVLIRRAYLDVLGLPPSP
                     DQVEAFL"
BASE COUNT          716 a         1486 c         1428 g          678 t
ORIGIN      
        1 gaagccaacc tgcgctacac ctggagcctg caaggaacgc cgcccgccgg cgtggcgttc
       61 tcggtcaatg gaaataacgc ggccaaaaac acgacggtga cgttcgccca ggcagggacc
      121 tatcagttcg ccgtcgcgat ccgcgatgcg gacaaccaga tcgcggtcag cagtgcaacg
      181 gtgacggtcg tccagacgct gtccaacatc tccgtcagcc cggccagcgc caccgtcttg
      241 aagagcggca cgcggcagct tacggcgacc tcgcgcgacc agttcggcaa tgtcctgacg
      301 acgcaaccgg cgatcacctg gagcatcgac gccggcggca tcggctcggt cagcgccgat
      361 ggcctctatc aggcccccgc ggaagccggc acggccgtcg ttcgggcgac ggccggctcg
      421 ttcagcgggt cagcgccatt accgtcacgt cgattgtgcc ggacaccggc aacggcctgg
      481 ccgccgtcta ttacaacaac atcaacctca cgggcacgac gctcacccgc acggacgcga
      541 cgatcaattt cgactttgcc accggctcgc ccggggcttc catcgccccc gacaccttct
      601 ccgcccgctg gatcggccaa atcgagccgc gattctccga gacttacacc ttcacgacta
      661 ccagcgacga cggcgtgcgg ctgtgggtga acaatcagct gctcatcgac cagtggaacg
      721 accacgcggt caagcagcac agcggcacga tcgccctcgt cgctggcgag cggccggcga
      781 ttcgcctgga gtattacgaa aacaccggcg cggcggtcat caagctccag tggcaaagcg
      841 ccagccagcc gcgtgagatc gtgccccaga gccggctcta tagcggtccg ctcgatccga
      901 tcttgaccag catcgccgtt tctcccgcca gcgcgaatct ggcgccgggc acgacgactc
      961 agttcacggc aacggccctg gaccagttcg gcaatgccct gcaggcccag ccggcgctgg
     1021 cctggagcat cgacgccggc ggcatcggag tggtaagcgc cgccggtctc taccaggccc
     1081 ccgtcacggg aagcggctcc gccatcgtcc gggcgtcggc cggcgcgatc ggcggcgtcg
     1141 ccaccgtcac cgtgggcacg gccgccgccg gcgccggcaa cggcctggca gccgtctatt
     1201 tcaacaacag taacctgacc ggcacctcga tcgtccgcac ggaccaagcg gtgaatttcg
     1261 atttcgccag cggctcgccg gcgagcttca tcgctgccga cactttttcc gcccgctgga
     1321 tcggccagat cgagccgcgg ttctccgaga cctacacctt caccaccacc agcgacgacg
     1381 gcgtgcggct gtgggtcaac gatcgactgc tcatcgacca gtggaacaac cactccgccc
     1441 ggcagcacag cggcacgatc gccctcgtgg ctggcgagcg gccggcgatc cgcctggagt
     1501 actacgaaag cagcggcctg gcggtcatca agctgcagtg gcaaagcgcc agccagcccc
     1561 gcgagatcgt gcccaagagc cggctctatg gcggcgcgcc cgttcgcgtg aattttcagc
     1621 cggcaggggc gcccgttcca accggctatg tcgtggacgc gggcgagacg ttcgccgccc
     1681 gagccagcgg ctactcgtac ggctggaacc tcgacaacac gctttcaccg cgcgaccgca
     1741 acagctcgct cgcgcccgat cagcggtacg atacgctcac gctgttgcaa acgccggaga
     1801 atcccggcgc ggcttgggag atcgcggtcc ccaccggagc ctatcgcgtc cacctggtgg
     1861 ccggcgacgc cggcagcctc gctggcgtct tcaagacgag cgtcgaaggc gtgctgattg
     1921 tcgatgggac gccgacgtcg ggccagcgct ggctggaggg ctggcgcgac atcctcgtca
     1981 gcgacggccg cctgacggtc acgagcgccg ccggcgcatc cggcaacaag ctcaacttcc
     2041 tcgacatcat tccgctgtag ccgcagcgcc tctggcgact tccgcggtga gggtgctatt
     2101 ctgatttccg gggttctcgc cggtcttatc cgctagaggc gccgcatggc cgcaacctgg
     2161 aagcagttga ccgatttcct ggtcgagcag gggatcgaac aaatcgccca cacccagaaa
     2221 aactatctgg cccacctgat cagcgtctac aagctgatgg aggccagcgg ctatggtgaa
     2281 gaactctgcc gggccgggct gttccactcg atctacggca ccgagaagtt ccagggcttc
     2341 aagctgccgc tgacgcgccg ggcagagctg gccgaaatga tcggcagccg ggccgagcgc
     2401 ctggcctact ggaactgcca gatggaccgc gcttcgctcg acgggctgct cacgcaggat
     2461 caagaaccgc tcctccttcg caaccgcgag accggcgagg aaatgccgct gacccgccgc
     2521 gagctggacg acctctgctg cgtgcacctg ttcgattggc tcgagcaggc cccccgctcc
     2581 cgcttcggct gggactaccg ccgccaagcc taccggcaga tggccgagcg cctcggcggc
     2641 gccgctctgg ccgcctacga ccaggtcttc gcggccgcgc cagcgggtgc aacataagcg
     2701 gagacgttag ttgacgacgc cgtttctttc agccaacgag gtggggacgg cgggagcgcg
     2761 gacgctgggg ggcgagtttt acacttcgcg ggaaatcttt gagcgcgagc gggagcggat
     2821 tttctcgcgg cggtgggttt gcgtggggcg cgagcagcag ctggcgggcg gcggctattt
     2881 tctgcgggat gtcgcgggcg agagcctgat tctgcttcgc gaccaggccg ggcaggttcg
     2941 ggcgcactat aacgtctgcc ggcaccgggg ctcgcggctt tgcgaagagg ccagcggccg
     3001 actggcggcg acgatccagt gcccctatca cgcctggacg tacgcgctcg acgggcgatt
     3061 gctcggcgcg cccggaatgg aagccctcgc gggcttcgac aagtcggccc acggcctcgc
     3121 cgctgcggcc gccggcgtct gggaaggttt cctctttgtg tcgctggccc agcagccgga
     3181 gccgctcgag caggccttcg cggcgctggc ggggaaattc gcccgctacg agctgccgcg
     3241 actggcgagc gtgcggcgga tcgactacga cgtgcgggcc aactggaagc tgatcgtgca
     3301 gaactactcc gagtgctacc actgcccggg cgtgcatccg cagctggtga agctgtcgcc
     3361 cgccgacagc ggcaagaacg atctcaccag cggcccgttc ctgggcggat acatgtcgct
     3421 accggccggg gccgcgctga ccaagagcgg ccggcgctgt gctatcgacg tcggcccgct
     3481 aacggacgag gaccggcaac gcgtctatta ctactcgatc ttcccgaacc tcttgctgag
     3541 cctgcacgcc gactacgtga tggtccatac gctctggccg ctcgcgccgg accggacgcg
     3601 cgtcgagtgc gagtggctgt tttctcccga cgccgccggc cggaacggct tcgatcccga
     3661 cggcggcgtg cagttctggg acgagacgaa ccgccaggac tggcacgtct gcgagctgag
     3721 ccagcaaggc gtcggctcgc gggcctaccg gcccggtctt tattcgcccc gcgaaagcat
     3781 ctccgccgcc ttcgaccgcg aggtgctgcg ggccctggcg gagtaggccc cttctgccgg
     3841 aaggggcctg aggcgaagcg cagcgagcaa gttgccccag gtcctgcgcg gctgacgaaa
     3901 cctgcaagtt gacgaccgcc gggccgcgga ggacgataag gggtcttttc agagggcgct
     3961 ggcagcgcgc ggagcctggc gatgaagcgc acgtccagtt cggctggctg gttatgctgt
     4021 gccgcgctcg tgctgttctg ctgcgcggca cgggcgcagc tgccggaccg cccgatcagt
     4081 cctgcggagc ggcagttctg ggcttttcag cagccgcggc gggtggaaac gccggcggcg
     4141 agcgcagcgg gctgggagca gaatgcgatc gatgcgtttc tgctggcggc aatgaaagcc
     4201 aaggaggtga ggcccgcgcc gccggccggc cgccgggtgc tgattcgccg cgcttacctc
     4261 gacgtgctgg gcctcccgcc gagtccggac caggtcgagg cgttcctc
//
LOCUS       DASRJB010000222         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4013737, whole genome shotgun sequence.
ACCESSION   DASRJB010000222 DASRJB010000000
VERSION     DASRJB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013737"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..452
                     /locus_tag="VFB80_06155"
     CDS             189..452
                     /locus_tag="VFB80_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33381.1"
                     /translation="MSDRDKDHDGGGLAVVVLAGFLALLLIVAAGGGWLYLRHLQQQR
                     INELVMEAELAEYNAQQAAASLAAAEAAANAAAAVLDAKAEEP"
     gene            complement(636..3713)
                     /locus_tag="VFB80_06160"
     CDS             complement(636..3713)
                     /locus_tag="VFB80_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33382.1"
                     /translation="MRRHDTKEALYSRAGMAAIELAVLLGGVSVGLLFFAASGVIVFR
                     TLNQKPQVIAQVPQVTPPVVATPTTNPPTPAVTPVPPQVTPPAVTPPTPPATTPPAPA
                     SRIGNLELTFGPGVDVLHQGPITKHDGQFKLDSTGLATDATGPCKLEFPCELTGNYLI
                     EINFTRLSGTDSLNTQFNIGGHDCSFVLDGFSERGGASGIDMVDVVRPLNRRGAVKGL
                     LVTNGSPSRLRLAIVENRMIAELNGRVLEDWSGDPARLRLYVGGEFGQPHRLRLVTWH
                     SGYQITRIEMTPIVAASPAGGASSSPPLAGTAPPPPAPTPSAVFEQLGIKVGEPINLL
                     QRVSGSPNTFRREASQMVTEERVLCKLTFLTTVPESYVVQARVKRNSPLESIAISFPL
                     AANRGAWVIDGWKPSGGPFSGLEMIDGRRVNENPTRVSGQQIADGKLADLLLVVLPGR
                     IVSQIDGKQILDWKGNPSQLSETAFTKISRPQHVGIVTHFASYTIESALLYPITDAGQ
                     LLAAAPQTTPMPPATTPIPPPATNPPAAANPPAVAQAKRLPVPDEAAQKPIRDNLASV
                     FGDRLAKAKKPEERAALAREMADVVKSEPDATTQYVLLTAARKTALNGLDFSLAMELA
                     VSTAAKFDVEPVALRLETLTQAAALAGSNPDAHKSLAELAGTFAEETLAGERYDVADT
                     ASKLASDAGIKSKDADSRRASKELRDEVLAEKKMYDATQAALETLKTQPKDAASNLVV
                     GKYYSLAREDWARALPHLAAGGDPALSPAAEAEQQAKDAPGQLAAAEKWQAAANAAKG
                     PERADLARHALALCDAALPQLSGLPKVRAEKCQGELQTLIADSEKSTGGTAAATTRRT
                     RSRSGKLEPGLIVRTYRGDLSRPSAALSIVMSSDEYYQRALDAVKTIIFRGYPNDYYL
                     PVAMGYIVLDQPGTVELELENCTCLVNGAKFAEETGSTSTKVKRPLSKGRHLVAFTRA
                     GSFYAPKFNVTLEGSDKPVLYHAPQDLKIELARCVYVNGDPYFGMLVYRTPQK"
     gene            complement(3820..4380)
                     /locus_tag="VFB80_06165"
     CDS             complement(3820..4380)
                     /locus_tag="VFB80_06165"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN33383.1"
                     /translation="MSVGLKISFDEYTEMVADGAFKALRDRRVELIRGELCEMNPPGP
                     DHSEAVTRLNEWSTEPSARKLVRIRIQEPIGIPELESAPQPDVAWVKVREYRDRHPLP
                     PEVLLLIEVADSSLDSDCGEKAELYAAAGIQDYWVVSLRERLIHVYRKPRGGAYSQHC
                     TAGFGEELRPLAFPQYSLSVAELLGS"
     gene            complement(4439..>4561)
                     /locus_tag="VFB80_06170"
     CDS             complement(4439..>4561)
                     /locus_tag="VFB80_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D ubiquinol oxidase subunit II"
                     /protein_id="HZN33384.1"
                     /translation="LERDEADLAHLPRLVFQFNRRNLGRLRLLIDAINRGSVQA"
BASE COUNT          727 a         1445 c         1543 g          846 t
ORIGIN      
        1 aagaagcgca cgacaatttg ccctttgcaa ttctcaattg gcactttgca atcccggcaa
       61 aatgcaaaat acaaaatgca aatctcaaat tgctggtacc gtaccgcgga caaaaaaatc
      121 ccaccagccg aatcgtgggc cgggagcgtc caattgcttt ctccagcggc cgcgggcaga
      181 ctggcggtat gagcgaccgc gacaaagacc atgacggcgg cgggctggcc gtcgttgtgc
      241 tggccggctt tctggcgctg ctgctgatcg tggctgccgg gggcggctgg ctctacctgc
      301 ggcacctgca gcagcagcga atcaatgagc tggtcatgga ggcggagctc gcggaataca
      361 acgcccagca agcggcagcg tcgctggccg ccgccgaggc ggcagccaat gcggcagcgg
      421 cggtgctcga tgccaaggcc gaagaaccct agctgtctgg cgtgagccga cggttcgatc
      481 gcgctcacca ctttgtactt tgcaattctc aatttgcact ttgcactttc tccgcctgtc
      541 tggcagagat tgcaaattgc aaagttcaaa gtgcaaagtg ttcaggagac tgggccgggc
      601 actggcgcaa cgccagtgcc accttgcgca aaatgctatt tctgcggcgt ccggtagacc
      661 agcatgccga agtaggggtc gccgttgacg tagacgcagc gggcgagctc gattttgagg
      721 tcctgggggg cgtgatagag gaccggcttg tcgctgcctt cgagcgtgac gttgaacttg
      781 ggggcataga aactgccggc ccgcgtgaag gccacaaggt gccggccctt ggagagcgga
      841 cgcttgacct tcgtgctggt gctgcccgtc tcctcggcga atttcgcccc gttgaccagg
      901 caggtgcagt tctccagctc cagctccacg gtccccggct gatccagcac gatgtagccc
      961 atcgccacgg gcaggtagta atcgttcggg tagccgcgga aaatgatcgt cttcaccgca
     1021 tcgagggccc gctggtagta ctcgtcggac gacatgacga tcgagagggc cgcgctgggg
     1081 cgggagaggt cgccgcggta ggtccgcacg atcagccccg gttccagctt gcccgagcgg
     1141 ctgcgggtcc gacgggtcgt agccgcggcc gtgccgccag tggacttttc gctgtcggca
     1201 atcagcgttt gcaattctcc ctggcacttt tccgcccgga ctttgggcag gcccgagagc
     1261 tgcggcaggg cagcgtcgca cagcgccagc gcgtgccggg caaggtcggc ccgctcgggg
     1321 cctttggcag cgttggcggc cgcctgccat ttctcggcag ccgccagctg ccccggggcg
     1381 tccttggcct gctgctcggc ctcggcggcg ggcgacagcg ccgggtcgcc gccggcggcc
     1441 aggtggggca gggcccgggc ccagtcctcg cgggccaggc tgtagtactt gcccacgacg
     1501 aggttgctgg cggcgtcctt gggctgcgtc ttgagcgtct ccagcgcggc ctgcgtagcg
     1561 tcgtacatct tcttctcggc cagcacttcg tcgcgcagct ccttgctcgc gcggcgcgag
     1621 tcggcgtcct tcgacttgat cccggcgtcg gaggcgagct tcgaggcggt atcggccacg
     1681 tcgtaccgct cgccggccag cgtctcttcg gcaaaggtcc ccgccaattc ggctagcgac
     1741 ttgtgggcgt cggggttgct gccggcgagg gccgcggcct gcgtgagcgt ttccaggcgg
     1801 agggctacgg gctcgacgtc gaacttcgcc gcggtggaga cggccagctc catcgcgagg
     1861 ctgaagtcca gcccgttcag cgccgttttg cgggccgcgg tgagcagcac gtactgcgtc
     1921 gtggcatccg gctcgctctt gaccacgtcg gccatctcgc gggccaatgc ggcccgctcc
     1981 tcgggcttct tggctttggc cagccggtcg ccgaacacgc tcgccaggtt gtcgcgaatc
     2041 ggtttctgcg ccgcctcgtc cggcacgggc agccgcttgg cctgggcgac ggccggcgga
     2101 ttcgcggccg cgggcggatt ggtcgccggc ggcgggatcg gcgttgtcgc cggcggcatc
     2161 ggtgtcgtct gcggggcggc ggccagcagc tggcccgcgt cggtgatcgg gtagagcagc
     2221 gcgctttcga ttgtgtagct ggcaaagtgc gtgacgatgc cgacgtgctg cgggcgggaa
     2281 atcttggtga acgccgtctc cgagagctgc gaggggttgc ccttccagtc gaggatctgc
     2341 ttgccgtcga tctggctcac gatgcggcct ggcagcacga cgagcagcag gtcggccaat
     2401 ttgccgtcgg cgatctgttg gcccgagacg cgcgtcgggt tttcgttgac gcggcggccg
     2461 tcgatcatct ccaggcccga aaacggcccg ccgctcggct tccagccgtc gatgacccaa
     2521 gcgccgcgat tggccgccag gggaaagctg atggcgatcg attccagcgg tgagttgcgc
     2581 ttcacgcggg cctggacaac gtagctttcc ggcacggtgg tgaggaaagt gagtttgcag
     2641 agcacgcgct cctcggtgac catctggctc gcttcacggc gaaaggtgtt cggcgacccg
     2701 ctgacccgct gcagcagatt gatcggctcg ccgactttga tccccagctg ctcgaagacg
     2761 gcgctcggcg tcggggctgg aggcggcggg gccgtgccgg ccagcggcgg actgctgctc
     2821 gcgccgcccg cggggctggc cgcgacgatc ggcgtcatct cgatccgcgt gatctggtaa
     2881 ccggaatgcc acgtcaccag ccgcaggcgg tgcggctggc cgaattcgcc gccgacatag
     2941 agcctgagcc gtgccgggtc gccgctccag tcttccagca ctcgcccatt gagctccgcg
     3001 atcatacggt tctcgacgat cgccagccgc aggcggctag ggctgccgtt ggtcactagc
     3061 aggcccttga ccgcgccccg gcgattgagg ggccgcacga cgtcgaccat atcgatgcct
     3121 gacgcgccgc cgcgctcgct gaagccgtcg agcacgaacg agcagtcgtg gccgccgatg
     3181 ttgaattgcg tattgagaga gtcggtcccg ctgaggcgcg tgaaattgat ctcgatcagg
     3241 tagttgccgg tcagctcgca ggggaactcg agcttgcagg gaccagtggc gtccgtggcc
     3301 aggcccgtgc tgtccagctt gaattgaccg tcgtgcttcg tgatcggccc ctggtggagg
     3361 acgtccacgc ccgggccaaa cgtcagctcc agattgccga tgcggctggc aggcgctggc
     3421 ggggtggtcg ccggcggagt cggtggggtg accgcgggag gcgtaacttg cggaggaacc
     3481 ggcgtcacgg ctggcgtcgg tggattggtc gttggtgtcg ccacgaccgg cggggtcacc
     3541 tggggaacct gggcgatgac ctggggcttc tggttgagcg tccggaaaac gatgacgccg
     3601 ctggccgcga aaaacagcag gcccacgctg acgcctccga gcagaacggc cagctcgatg
     3661 gccgccattc cggcgcgcga atagagcgcc tccttggtgt cgtgccgacg catgctgcac
     3721 agctcccagg attttcccca agaccgttag atttacccta gtcgagacgg agcaggggcg
     3781 tcaatcgttc tgcaccgact gttacgaacc gcatggcagt cacgatccga gcaattcggc
     3841 gacgctcagg ctgtactgcg gaaaggccag cggccgcaat tcctcgccaa atccggcggt
     3901 gcagtgttgc gaataggcgc cgccccgagg cttgcgataa acgtgaatga gtcgctcccg
     3961 caaactcact acccaataat cctgaatccc tgctgcggca tacagctcgg ccttctcgcc
     4021 gcaatcggaa tccaggctgg agtcggcgac ttcaatcagc agcagcactt ctgggggcag
     4081 cggatggcgg tcgcggtatt cgcgcacttt gacccacgcc acgtccggct gtggcgcgga
     4141 ttctaattcc ggaattccga tcggctcctg aatccgaatc ctcacgagtt tccttgcgga
     4201 tggctcagtg ctccactcat tgagcctcgt cactgcttcg ctatggtccg gtccgggcgg
     4261 attcatttcg cacaattctc ctcggatcag ctcgacgcgg cgatcgcgca gggccttgaa
     4321 cgcaccatcg gcgaccatct cggtgtattc atcgaagctg attttcaggc cgacactcat
     4381 ccgcgaaccc tccaatccgt atcatagccc gggggtctaa agccggcaaa tcgcgacgtc
     4441 aggcttggac gctgccgcgg ttaatggcat cgatgagcag ccgcaggcgc ccgaggttgc
     4501 ggcggttgaa ctgaaagacc agccgcggca gatgggccag gtcggcctcg tcccgctcca
     4561 g
//
LOCUS       DASRJB010000223         5829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2601928, whole genome shotgun sequence.
ACCESSION   DASRJB010000223 DASRJB010000000
VERSION     DASRJB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601928"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFB80_06175"
     CDS             <1..734
                     /locus_tag="VFB80_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33385.1"
                     /translation="AAQLLGKGWLVPLTNGSVVLVDSKGGPAAQPFVPPLQPSSLPLW
                     TRPAVNAAGDGALVSDGRGTIYSIALKVQPQPHLASLASSQTDGPVTSPLVLAGSTYF
                     GVLSRESGDALAGFDAQGQSAFAPVALPGRCQAGPFAAGGLVLVATESAGLTAYEGSG
                     KQRWQQPLAAGPLAGPPVICPEGDLLVIHQSGAVRRIDPASGAELAAVNVAQPLGAAA
                     RILGQNVFLAGSDGVVHRITLPPRP"
     gene            744..3107
                     /locus_tag="VFB80_06180"
     CDS             744..3107
                     /locus_tag="VFB80_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN33386.1"
                     /translation="MTAGAKCERVRRWACWLAAAAWFALAPHAIAQNEAPRRLIDEAP
                     YDVLTLDKSNESKVLKVFPVNLPGRKLPEARKPTDKLKVKLLADGVDYEVMWQHVEKL
                     EFFEQMVLAEAVQLTTAGKFDEAYDYFAYLLARYPDTPGLPEARQSYLYLAAGSAFRQ
                     QKHDEALAVLEELLVQNPAYRSGENSPTLMQVLGSIADRILSRYVEKQDYRSARLLLA
                     RLSQQYRADNEPFAQRWRQQLTDLAQKRLSEAQAHLAASRYLEAHDAIVAMKLVWPDL
                     PGGAQVAAEIARRHPLVTVGVEHPALEFDSLSLHNVAARRAGRLSERLLVELTGLGPE
                     GGNYVSPYGAVSRSEDGLELIFRLPPATAGSTAAYDLAQRLLSRAQPTGAEYQPTWAR
                     IVASVRAPTTNEVRVELRLAHVLPEALVQVPLGVAEDGKAGTVTRMTPFAQLSRDEQQ
                     ARFTANEQYVFRVATQPAEIIERQFTDPQRALLALKRGEIDVLDRVFPGDIASLTGDS
                     AIVVAPYRAPTTHVLAIRSEHPYLANRNFRRALLYGSNREQLLTQGLLRGQSLPGFRT
                     VSAPFPAPAPDLPAYGYDQQIAPREFDPRLGLTLRVVAEGEIKSTNERRMLPVPKLTP
                     IVLGHPADEAARIACRGLARQWKTIGVECKLLEFPPGVFDDQEKKCDLVYLQLAAWEP
                     LVDAGRLLGAGGVAPATSAVIQLTLREIEQARNWQQARERLLHLHRLVHEDVAVLPLW
                     QTMDHFAYRRSLTGLAPDRLRLYQDVEQWRTAPQPAAVAAQRAGAQP"
     gene            3104..4567
                     /locus_tag="VFB80_06185"
     CDS             3104..4567
                     /locus_tag="VFB80_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33387.1"
                     /translation="MSGGARSSVAAGLLLVFLCAQPRTGLGQAAWEFSPYRVRVRLAL
                     EPVPQLPADIAPQLGKFISARAHTVLGAVWQVESLVAPPALRDELLRGGEISPEAIAA
                     ALPDDLAGDKIYLAAISYRAGGYRVAVRELDCRTRQLSDAFERFAPDMADLGTALWDA
                     ALEAFTPVAKIETVDQTRITARLRAGGLIVDPASRGLVEKGMVLRPVIRRNDRSGQPA
                     KLGIQVLPWTLLSVNERRDSLLDTTFHSAFRLAIPSRGSSRTERLALLVRPRWPSTRL
                     ELRSRGDAAKPLAGYELHIKVPGGESEAEPTTEQKTELIGTTDADGAVELPRSHEMQL
                     LYVRSGKQLLARLPILPGQSQMLLASVVDDDRRLQAEGLILAMQSRALDLVARREILA
                     ARFRAKLKAGQLAEARQLLDAYRKMETRQDLSRDLDQYRQRVASTDRITQARIDKLFM
                     DAQQLLTLRPLSDEMLGQLNRELSAAESGGGAKASGS"
     gene            complement(4574..5704)
                     /gene="queG"
                     /locus_tag="VFB80_06190"
     CDS             complement(4574..5704)
                     /gene="queG"
                     /locus_tag="VFB80_06190"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZN33388.1"
                     /translation="MPLPPAILTDELRRRARRLGFALVGVCPAVAPSGVSRLNEWLER
                     GYAGEMDYLVARQEAYEHPRHVLDGVRSIVMLGLPYETEAPQQPRAGQGRISRYAFGS
                     GDYHDVIHDKLRLLIAALREIAPGAAARGVVDTAPLLEREFAQLAGLGWIGKHTLLIN
                     KPAGSYFFLAALLTDQELIADEPFAADHCGTCRACLDACPTGAFPQPYVLDATRCISY
                     LTIELRGPIPAGLRAGIGDWVFGCDVCQEVCPWNHRPQSASEPAFQPAEDANPVDLIA
                     LLELDDEGFRRRFRHTPLWRARRRGILRNAAIVLGNQRADSAAAALIRALEDAEPLVR
                     GAAAWALGRIGGEAARQALAERAALEPAGDVRREIEAALRGE"
BASE COUNT          926 a         2036 c         1996 g          871 t
ORIGIN      
        1 ccgccgcaca gctgctgggc aagggctggc tcgtgccgct gaccaatggc agcgtggtat
       61 tggtcgattc caagggcgga ccggccgcgc agccgtttgt gccgccattg cagccgagtt
      121 ccctgcccct gtggacgcgt ccggcggtga acgcggccgg cgacggcgcc ctggtcagcg
      181 acggccgcgg cacgatctat tcgatcgcgc tcaaagtgca gccccagccg cacctggcct
      241 cccttgcgtc gagccagacg gatggcccgg tgacttcgcc gctggtgctg gcgggctcga
      301 cttattttgg cgtgctgtcg cgcgagagcg gcgacgcctt ggcgggcttt gatgcccagg
      361 gccaatcggc gttcgccccg gttgctctgc ccggccgctg ccaggcagga ccgttcgcag
      421 ctggcggcct cgtgctcgtg gcgacggaat ccgcgggcct gactgcctat gaaggcagcg
      481 gcaaacagcg gtggcagcag cccctggcgg ccggaccgct ggccggtccg ccggtgattt
      541 gccccgaagg cgacttgctc gtcattcacc aatcgggcgc ggtgcgccgc atcgatccgg
      601 ccagcggggc ggaattggcc gccgtcaacg ttgctcagcc gctgggcgcg gcggctcgaa
      661 tcttgggaca gaacgtcttc ctggccggca gcgacggggt ggtgcaccgc atcaccttgc
      721 cgccgcggcc ctaggtggct ggcatgaccg cgggagccaa atgcgaacga gtccggcgct
      781 gggcctgttg gctggccgct gccgcctggt tcgcgctggc gccgcacgcg attgctcaaa
      841 acgaagcgcc gcggcgtctg atcgacgaag ccccgtacga cgtcctcacg ctcgacaaat
      901 cgaacgagag caaggtgctg aaggtctttc cggtcaacct tcccgggcgc aagctgccgg
      961 aagcgcgcaa gccgacggac aagctgaagg tcaagctgct ggcggacggc gtggactacg
     1021 aggtgatgtg gcagcacgtc gagaagctcg aattcttcga gcaaatggtg ctggccgaag
     1081 ccgtccagct gaccacggcc ggcaaattcg acgaagcgta cgactacttc gcgtacctgc
     1141 tggcccgcta tcccgacacg ccggggctgc ccgaagcgcg gcagagctac ctgtatctgg
     1201 cggccggttc cgcgttccgt cagcagaagc acgacgaggc cctggccgtg ctggaggaat
     1261 tgctcgttca gaatccggcg taccgctcag gcgagaattc gcccacgctg atgcaagtgc
     1321 tcggcagcat tgccgaccgg attctctccc gctacgtcga aaagcaggac tatcgctcgg
     1381 cccggctgct gctggcccgc ctgtcgcagc aataccgggc cgacaacgag cccttcgccc
     1441 agcgctggcg gcagcagcta accgatctgg cgcagaagcg cctcagcgag gcccaagcgc
     1501 atctggcggc cagccgttat ctggaggcgc acgacgccat cgtcgcgatg aagctggtct
     1561 ggccggactt gcccggcggc gctcaggtcg cggccgagat cgcccgccgc catccgctgg
     1621 tgacggtcgg cgtggagcac ccggccctgg agtttgacag cctcagcctg cacaacgtcg
     1681 ccgcccgccg ggccggacgc ctcagcgagc ggctgctggt cgagctcacc ggattggggc
     1741 cggaaggcgg caattacgtg tcaccctacg gcgcggtcag ccgcagcgag gatggcctgg
     1801 agctgatctt ccgcctgccg cctgccacgg cgggcagcac cgcggcctac gacctggccc
     1861 agcggctgct cagccgggcg cagcccacgg gggcggaata tcagccgacg tgggcccgca
     1921 ttgtcgccag cgtgcgcgcg cccaccacga acgaagtgcg ggtcgaactg cggctggccc
     1981 atgtgctgcc cgaggcgctg gtgcaggtgc cgctgggagt ggccgaggac ggcaaggcgg
     2041 gcacggtgac gcggatgacg ccctttgctc agctctcgcg cgacgagcag caggcccgct
     2101 tcacggcgaa cgagcaatat gtcttccgcg tcgccacgca gcccgcggag atcatcgagc
     2161 ggcaattcac cgatccgcag cgggccctgc tggccctgaa gcgcggcgag atcgatgtcc
     2221 tcgaccgcgt attcccgggc gacattgcgt cgctgacggg cgattcggcg atcgttgtcg
     2281 cgccgtaccg cgccccgacg acgcacgtgc tggcgatccg ctcggagcat ccctacctgg
     2341 cgaaccgcaa ctttcgccgc gcgctgctct acgggagcaa ccgcgaacag ttgctgacgc
     2401 agggcctgct ccgcggacaa tcgctgcccg gattccgcac cgtgagcgcg ccgtttcccg
     2461 cgccggcccc cgatcttccg gcgtacggat acgaccagca aatcgccccc cgcgaattcg
     2521 atccgcggct ggggctgacg ctccgcgtcg tggccgaagg ggagatcaag tccacgaacg
     2581 agaggaggat gctgccggtg ccgaagctga cgccgattgt gctcggccat cccgccgatg
     2641 aagccgcgcg gatcgcctgc cgcggcctgg cccggcaatg gaagacgatc ggtgtggagt
     2701 gcaaactgct ggagtttccg cctggcgtgt tcgacgacca ggagaaaaag tgcgacctcg
     2761 tctatttgca actcgccgcc tgggagccgc tcgtcgatgc cggccgcctg ctgggagccg
     2821 gcggcgttgc accggccacg agcgcggtca ttcagctgac gctccgcgag atcgagcagg
     2881 cccgcaactg gcagcaggcc cgcgagcggc tgctgcacct gcatcgcctg gtgcacgaag
     2941 acgtggccgt gctgccgctg tggcaaacga tggatcattt tgcctaccgc cgctcgctga
     3001 ccgggctggc gccggaccgc ttgcggctgt atcaggacgt cgagcagtgg cggaccgccc
     3061 cgcagcccgc cgctgttgcc gcgcagcgcg caggagcgca gccatgagcg gcggcgctcg
     3121 ctcgtccgtc gcggcaggac tgctgctggt attcctctgc gcgcagccca gaaccggact
     3181 cggccaggcc gcgtgggaat tttcgccgta ccgcgtgcgc gtgcggctgg ccttggagcc
     3241 tgtgccgcaa ttgccggcgg acatcgcgcc acagctgggc aaattcatct ccgcccgcgc
     3301 tcatacggtg ctgggcgccg tctggcaggt cgagtcgctc gtcgcgccgc cggccctccg
     3361 cgacgagttg ctgcgcgggg gcgaaatttc gcccgaggcg atcgccgcgg ccctgcccga
     3421 cgacctggcc ggcgacaaga tctacctggc ggcaatctcg taccgcgcgg gcggctatcg
     3481 cgtcgccgtg cgcgaactgg attgccgcac tcggcaactg agcgacgcct tcgagcgctt
     3541 cgcaccggac atggcggacc ttggaacggc cctctgggac gcggcgctgg aggcatttac
     3601 gcccgtggcc aagatcgaaa ccgtggacca gacgcgcatc acggcccggc tccgtgcggg
     3661 cggcctgatc gtcgatcctg cgtcgcgggg actcgtcgaa aagggaatgg tcctgcggcc
     3721 cgtcattcgc cgcaacgacc gctcggggca accggccaag ctcggcatcc aggtcctgcc
     3781 ctggacgctg ctctcggtga acgagcgccg cgattcgctc ctggatacca cgttccattc
     3841 cgcgttccgc ctggccattc ccagccgcgg cagctcgcgg accgagcggc tggcgctgct
     3901 cgtgcggcct cgctggccca gcacgcggct ggagctgcgg tcgcgaggcg acgccgccaa
     3961 accgctggcc ggctatgaac ttcacatcaa agtgcccggc ggcgagtcgg aggccgagcc
     4021 gacgaccgag cagaagaccg agctgatcgg caccaccgac gccgacggcg cggtcgagct
     4081 gccccgctcc catgagatgc aactgctgta cgtgaggagc ggcaagcagc tgctggcccg
     4141 gctgccgatc ctgccggggc agagccagat gctgctcgcc agcgtcgtcg atgacgaccg
     4201 ccgcttgcag gccgaggggc tgatcctggc aatgcaaagc cgggccttgg acctggtggc
     4261 ccgccgcgaa atcctcgcgg cccgcttccg cgccaaattg aaggcggggc agctcgccga
     4321 agcgcggcag ctgctcgacg cgtaccgcaa gatggagact cggcaggacc tgagccgcga
     4381 cctggaccag taccgccagc gtgtcgcgtc gacggacagg atcacgcaag cccgcatcga
     4441 caagctgttc atggacgccc agcagctgct gacgctgagg ccgctcagcg acgagatgct
     4501 cggccagctc aatcgcgagc tctccgcggc cgaatccggc ggcggggcca aggcgagcgg
     4561 cagctgagcc ccgctactcg ccgcgcagcg ccgcttcgat ctccctgcgc acgtcgccgg
     4621 cgggctcaag ggcggcccgt tcggcgagag cctggcgggc ggcctcgccc ccgatccggc
     4681 ccagggccca ggccgccgcg ccgcgcacca gcggctccgc gtcctccagc gcgcggatca
     4741 gcgccgccgc ggccgaatcg gcccgctggt tgcccagcac gatcgcggcg ttgcgcagga
     4801 tgccgcgccg ccgcgcccgc cacagcggcg tgtggcggaa gcggcgtcta aagccttcgt
     4861 cgtccaattc cagcagggcg atcaaatcga ccgggttggc gtcctcggca ggctgaaagg
     4921 cgggctcgct cgccgactgc gggcggtgat tccaagggca aacctcctga cacacgtcgc
     4981 agccgaacac ccaatcgccg attccggccc gcagcccggc cgggatgggg ccgcggagct
     5041 cgatcgtcag atagctgatg cagcgcgtag cgtcgagcac gtagggctgt ggaaacgcgc
     5101 cggtcgggca ggcgtcgagg caggcccggc aagtgccgca gtgatcggcg gcaaagggct
     5161 catcggcaat cagctcctgg tcggtgagca aggccgccag gaagaagtag ctgcccgcgg
     5221 gcttattgat cagcagcgtg tgcttgccga tccagcccaa gccggcgagc tgggcgaact
     5281 cgcgctcgag caggggcgcg gtatcgacca cgccgcgggc tgccgcgccc ggcgcaatct
     5341 cccgcagggc cgcgatcagc agccgcaatt tgtcgtggat cacgtcgtga taatcgccgc
     5401 tgccaaaggc gtagcgcgaa attcgcccct ggcccgctcg cggctgctgc ggcgcttcgg
     5461 tttcgtacgg caatcccagc atcacgatgc tccgcacccc atccagtaca tgccgcggat
     5521 gttcgtacgc ctcctgccgc gctacgagat agtccatctc gcccgcgtag ccgcgctcga
     5581 gccattcatt cagccgcgag acgccgctgg gcgcgaccgc cgggcagacg ccgaccagcg
     5641 caaaacccag ccgccgcgcc cggcggcgca gttcgtcggt caggatggcc ggcggcaaag
     5701 gcatagggtt cagggttcag ggttcagaga aggcagccgt agaggtctcg tggactatgt
     5761 acgaactgcc ccgtcccgca ataattgcat gttgtgcacc gcaatcaaac accacgcagg
     5821 cgcaacggc
//
LOCUS       DASRJB010000224         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4014320, whole genome shotgun sequence.
ACCESSION   DASRJB010000224 DASRJB010000000
VERSION     DASRJB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4014320"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFB80_06195"
     CDS             <1..1293
                     /locus_tag="VFB80_06195"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZN33389.1"
                     /translation="VADPHSAAAVNVREARRQHQRLSRLPRSLVEELARVTTAAQHEW
                     KLARAASDFKHFEPWLAAIITLKREQALCLAEAGPLYDPLLAEYEAGLRTADVAAILE
                     VLRSELTSLLWEVLEMQARAQAASPPGLRGHFPDAAQRQLCRSLAEQLGFDFRRGQLD
                     TAVHPFSTHLGPHDIRMAIRCNECDLRESLYALLHETGHALYDQGLPSEHFGTPAGDA
                     ISLGVHESQARLWEISVGKSRGFWRFLLPQLTAAFPAPAGADSNLVWREVNRVQPGVQ
                     RVGADEVTYNLHIVLRFELEQALLSRELPTADLPAAWNERQQALLSVRPASDREGCLQ
                     DGHWAAGMFGYFPTYTIGNVLAAQLFERAAAELGNLEDQFAAGDFRPLREWLAVHVYR
                     HGKRLTTAELCQGVCGRGLDPAVLIGGLRRKHTALWGS"
     gene            complement(1290..1454)
                     /locus_tag="VFB80_06200"
     CDS             complement(1290..1454)
                     /locus_tag="VFB80_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33390.1"
                     /translation="MNDDVLLTLHCPKCAELVEVVAEGNNLVCLECGWKFDDRIAAKF
                     LNPQPQEENA"
     gene            complement(1562..1915)
                     /locus_tag="VFB80_06205"
     CDS             complement(1562..1915)
                     /locus_tag="VFB80_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HZN33391.1"
                     /translation="MPQLVTMGAQLACVQGSAPSSLIVVDPTVTGEVKLAANIMDHKP
                     IVNIPPFGTCTVLTTAASGVATPCVPAITAPWTPGNPTVMIRGQPALNNSSTCTCTIG
                     GAISITNPSATKEMV"
     gene            complement(1993..>3334)
                     /locus_tag="VFB80_06210"
     CDS             complement(1993..>3334)
                     /locus_tag="VFB80_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZN33392.1"
                     /translation="GVGPDCLFLDVRGIGVLFGGEEALAREVVAETSRLGYEARVAIA
                     ETIGAAWAGTRVQGSGFGVQENAEAPTDSRLPTPAPLPLAVLRIPPETLDLLSQLGVT
                     QLDQLLALPRESLRSRFGERLLLRIDQFFGRAQETIAAHRPPPQFAAEWVLEYPAERR
                     EVVEQILEQLALRVARALADRREGAVQLHCRLDCAPGGPLFLEVGLFRPSAEPGHLWD
                     LLRMQLEQAALCCWGRAALRGPVGRVTLEARLTAPLENRQGELFAGGEHEIERQLALF
                     IDRCSSRLGADAVLRPELTADPLPERAMKWKQGVRSRGSGVGRQKWKAALGTEYSGRS
                     TKSRRRSAASSDSRPPTPDSQRSPLHRPLALYDPPLSLHTLSVVPDGPPVKFDFGGER
                     HTVSRHWGPERIETGWWRGRSVRRDYWRVETASGRRFWLFRSLQEGTWHLHGEF"
BASE COUNT          546 a         1133 c         1089 g          566 t
ORIGIN      
        1 gtggccgatc cgcacagtgc ggcggccgtc aacgtgcgcg aagcccggcg tcaacaccag
       61 cggctgtcgc gcttgccgcg aagtctcgtc gaggagctgg cccgcgtgac gacggcggct
      121 cagcacgagt ggaagctggc ccgcgcggcg agcgacttca agcattttga gccctggctg
      181 gcggccatca tcacgctgaa gcgcgagcag gcgttgtgct tggccgaagc tgggccgctc
      241 tatgacccgc tgttggccga gtatgaggct ggcctgcgaa cggcagatgt cgcggcgatc
      301 ctggaggtgc tccgaagcga gttgacgtcg ctgctgtggg aagtgctgga gatgcaagct
      361 cgtgcgcagg ccgcgtcgcc gccggggctg cgaggccatt ttcccgatgc ggctcagcgt
      421 cagctttgtc gctcgctggc cgagcagttg ggcttcgact tccgccgcgg ccagctcgac
      481 acggccgtgc acccgttctc gacgcacctc ggcccgcacg acattcgcat ggcgatccgc
      541 tgcaatgagt gcgacttgcg ggaatcgctg tatgccctcc tgcacgaaac gggtcacgcc
      601 ctctacgacc aggggctgcc cagcgagcat tttggcacgc cggccggcga tgcgatcagc
      661 ctgggagtcc acgaatcgca agccaggctg tgggagatct cggtcggcaa gagccgcggg
      721 ttctggcggt tcttgctgcc gcaattgacg gcggcatttc ccgcacctgc gggcgcagat
      781 tccaacctcg tctggcgcga ggtcaatcgc gtgcagccgg gcgtgcagcg ggtcggcgcc
      841 gacgaagtca cgtacaacct gcatatcgtg ctccgcttcg agctggaaca ggctctgctg
      901 tcgcgcgagc tgccgaccgc cgacctgccc gccgcttgga atgagcggca acaggcgctg
      961 ctcagtgtcc ggccggcgag cgaccgcgag ggctgccttc aggacggcca ttgggccgcc
     1021 gggatgtttg gctacttccc gacctacacg atcggcaacg tgctcgccgc ccagctcttc
     1081 gagcgggccg ccgccgagct cggcaacctg gaagaccagt tcgccgcggg cgattttcgc
     1141 ccgctccgcg agtggctcgc cgtacacgtt tatcgccacg gcaagcggct cacgacggcc
     1201 gagctgtgcc agggcgtctg cggccgcggt ctcgatccgg ccgtgttgat cggcggactg
     1261 cggcggaaac acaccgcgct ctggggaagc taggcgtttt cttcttgcgg ttgcggattc
     1321 aggaatttgg cggcaatccg gtcgtcgaat ttccagccgc actcgaggca gacgaggttg
     1381 ttcccctcgg cgaccacctc gaccagctcc gcgcacttcg ggcagtgaag ggtcagcagg
     1441 acgtcatcgt tcatggcagg ttctccctag ggaagcctac gccatgagcg attggcgagg
     1501 atgacggaaa ttcgggttgg ttggattatg ggacttaggg gactaatgcg accgatgtcc
     1561 atcacaccat ttccttggtc gcgctcggat tcgtgatcga aatcgccccg ccgatcgtgc
     1621 aggtgcaagt gctgctgttg ttgagcgcgg gttggccgcg gatcatgacc gtcgggttgc
     1681 cgggcgtcca gggagcggtg atcgccggca cgcagggggt cgcgacgccc gaagcggcag
     1741 tcgtcaggac cgtacacgtg ccaaacggcg ggatgttgac gatcggtttg tggtccatga
     1801 tgttcgccgc cagcttgacc tcgccggtca ccgtcggatc cacgacaatc aaactcgagg
     1861 gggcgctccc ctggacacag gcgagctggg cgcccatcgt cacgagctga ggcatggtga
     1921 ggtctcctga gtgaattctg ctgcgccgct gcgccaggat agcggattct tcgcctgcgc
     1981 gtaatcgagt ttttagaact cgccatgcag atgccacgtt ccttcctgca ggctgcggaa
     2041 cagccagaac cgccggccgc tggcggtttc gacccgccaa taatcgcggc gcacgctccg
     2101 cccccgccac cagcccgtct cgatccgctc cggcccccag tggcggctga ccgtgtgccg
     2161 ctcgccgcca aaatcgaact tgaccggcgg accgtcgggg acgacggaca acgtatgaag
     2221 cgagagcggc ggatcgtaga gcgcgagtgg gcggtgcagg ggcgagcgct gggagtcggg
     2281 agtcgggggt cgggagtcgg atgaggcagc ggatcgacgc cgcgactttg tactccgtcc
     2341 tgagtactca gtacccagtg ccgccttcca cttttgcctc ccgactcctg acccccgact
     2401 cctgaccccc tgtttccatt tcatcgccct ttccggcagc ggatcagccg tcagctccgg
     2461 ccgcagcacg gcatccgctc ccagccggct gctgcagcgg tcgatgaaca gcgccagttg
     2521 ccgctcgatc tcgtgctcgc cgccggcgaa caattcgcct tggcgatttt ccaggggcgc
     2581 ggtcagccgg gcctcgagcg tgacgcggcc gaccggacca cgcagcgcag cccgccccca
     2641 gcaacacagc gcggcttgtt ccagctgcat ccgcagcaaa tcccacagat gtcccggctc
     2701 ggccgagggg cgaaacaggc cgacctccaa aaacagcggc ccgccgggag cgcagtccag
     2761 ccggcagtgg agctgcacgg ccccttcgcg gcggtcggcc aaagcccgcg ccacgcgcag
     2821 ggcgagctgc tccaggattt gctcgaccac ctcccgccgc tcggccggat attccagcac
     2881 ccactcggcc gcgaattgcg gcggcggccg gtgcgcggcg atcgtctctt gcgcccttcc
     2941 aaaaaactga tcgattcgca acagcagccg ttcgccgaac cgcgaccgca ggctctcccg
     3001 cggcagcgcc aagagctgat cgagttgcgt gacgccgagc tgcgaaagca agtcgagcgt
     3061 ttccggtgga atccgcagga ccgccagcgg caggggggcg ggagtcggga gtcgggagtc
     3121 ggtgggagcc tccgcatttt cctgaacccc gaaccccgaa ccctgaaccc tcgtccccgc
     3181 ccacgccgct ccgatcgtct ccgcaatcgc cacgcgggcc tcatatccga gccggctcgt
     3241 ctccgcgacg acttcgcggg ccagcgcttc ctcgccgcca aacaatacgc caatacccct
     3301 cacatccaga aacaaacaat ccggcccgac cccc
//
LOCUS       DASRJB010000225        16588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_65324, whole genome shotgun sequence.
ACCESSION   DASRJB010000225 DASRJB010000000
VERSION     DASRJB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65324"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFB80_06215"
     CDS             <1..377
                     /locus_tag="VFB80_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33393.1"
                     /translation="SLKIEFPAPKPAASSSQQLCSMAYWLVSENCRKLLRNNNLRRVF
                     AAGRFSGVCAHRDEPQISRPPSAHNAHNAATQNFDASEVAVISCVEIHCRRFFCAYAR
                     IGVRQTIAAVFSLVEARLPETK"
     gene            374..973
                     /locus_tag="VFB80_06220"
     CDS             374..973
                     /locus_tag="VFB80_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33394.1"
                     /translation="MTEFPDTRDSLLMQVRSPANREAWDQFALIYRPVIYRLARRRGL
                     QDADAQDLAQQVLVSVASAIGRYEKSGESVRFRHWLRTVTRNAIVNALSRQPHDRAAG
                     GSSAADWLVEQPVADPEADAQVELEYRRELYLRAGRIVRRDVEPDTWRAFELTVIENE
                     SAGEAAARLQMQVGSIYAAKSRIMRRLREVVKELEQWEK"
     gene            970..5022
                     /locus_tag="VFB80_06225"
     CDS             970..5022
                     /locus_tag="VFB80_06225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33395.1"
                     /translation="MKLNLPASGCDAERLEALLCGRLSDAEQREAIAHLNDCGSCRQR
                     LQAQAAEPEAWSEAESLLRPCEFAAPAAGEARTPPAIQHVLDELGATDDPQMLGRLGG
                     YEVSGVVGAGGMGVVLKAIDKSLDRTVAIKVLAPHLATSGAARRRFAREAKAAAAVLH
                     PNVIAIHSVSNDESLPYLVMPYLRGQSLQKRLDAEGPLPVQEVLRIGAQIAAGLAAAH
                     AQGLVHRDIKPANILLEEGVERVTITDFGLARASDDASITHSGLIAGTPQFMSPEQAR
                     GDAVDQRSDLFSLGSVLYAACTGRPPFRAETSYGVLRRITDDEPTPIRDINPDIPDWL
                     CTVASRLMSKRPQERFESAQEVANLLEQCLAHVQQPTAVPLPQRLVSRPPSANSATPL
                     ATTFRYGVLAMIAALGLGLLAAFVWLAADPPEISGQWSGDEWGQVVLKKLESGAYEGT
                     YTDTFGKEAGQLELKWSAVERRFKGAWREGQDRSGKLAVRLVDGEIRGAWTADKESRI
                     GPGTPELADLLWKRSSGISTTLPPAAVAGKASAEPRERSTIMPGRLNYFTTGHSIKVA
                     CSADGKLIAVANGNPTRILQVGGISRVKGDWKPTADILDLETGKVVVSLQLATSEEQA
                     VLAATERNAHLEATALAFSPTGKIVAVGTSIGQVKFFDARTGELLRSLDHAAAKLADK
                     ETPDSWRSIARAMGSVTSLAFSPDGSLLAVCGQPFRQFSDAFDGVSRLGREITGPDRL
                     KVWEVETGKLKHDLVGHTYPDEVAFSTDGTLLASAGRWSSEGDDGSGVIFWNPQTGAK
                     VGRLKADANVITQAVAFSPGGKLVAVGSQTYSSATNDDNTTTISLASAPTGLVHWKHT
                     VAGWANPKAFSPHGVVVLCKKSILLLDAETGQVKREIKATDLSKEGRWDDLAVAGRWL
                     VIASADEYEGNVELMELAGPDAAAAARPAPQPKAEGAVLRQVRRFQTAGSIQSVAVSP
                     DGKRIAVAGGEESAPPNFDWKRQVEILDADTGQSVVKIRLSTKEEDAVLAATAGSYHF
                     EVLAFSPDGSVLAVGTSAGQVKLFNPRTGELVRSLDDEPAKRADKETPEKYKSLARAL
                     GSVNSLAFSPDGSLLAVCGSSFEDKPIVDDRLERTRLSVAGPGRLKVWDVKTGALKHD
                     LAGHDEHANAVAFSADGSLLASAGRGIGASGHESGVIVWDVASGKPRSTILIPANGGT
                     YAVAFSPTKKLLAIGSRNFDKENDTRRATVSVAYALSGVMEWQQTFSVYANPKAFLPD
                     GKSVAVMCGREAIRFFDAESGTVKYEIKAAHAVRPAGWNDFAVAPQGRLLVIGGEDRD
                     HKGIVEIWDLPAPAADPPARADSPGGLSPR"
     gene            complement(4996..5760)
                     /locus_tag="VFB80_06230"
     CDS             complement(4996..5760)
                     /locus_tag="VFB80_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZN33396.1"
                     /translation="MPTKTKTIDETVQRGGKTKSPAMADAAAVVAELQRMGSQKVKDG
                     MARFAIPSDKAFGVSVADLRKLARRLGQNHALAVSLWETGWYEARMLTAFIDDPLQVT
                     PVQMDHWQRNFDNWAICDALCFHLFDKTPHAWRKVKAWAGQRGEFQKRAAFALLWALA
                     AHDKRSGDEPFHQSLGLIEVAADDDRNFVKKAVNMALRAIGKRGDALHAAAVAVAERL
                     AASDRVTPRWVGKDALRELNSPSVKRRLSARRKSAR"
     gene            complement(5764..6861)
                     /locus_tag="VFB80_06235"
     CDS             complement(5764..6861)
                     /locus_tag="VFB80_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZN33397.1"
                     /translation="MAHEVTLITGDGTGPELSQAARMCLDATGVKINWDVQEAGVDVM
                     AKTGTPLPDSVLASVRRTKCALKAPITTPVGTGFRSINVHLRQELGLFACIRPCKYYP
                     GVRSYFSEIGVDIVIVRENTEDLYAGVEFEAGKPATADLIDFINKISTDKKIKTKAAE
                     TGVSIKPISRSGTERIVRCAFDYARQNKRKSVTAVHKANIMKYSDGLFLHTATAVAKE
                     YPDIKYEERIVDNMCMQLVQKPELYDVLVLPNLYGDIVSDLGAGLVGGLGVAPGENIG
                     PGGAVFEATHGSAPKYKGQNKVNPTALILSGMLMLRHLGEMDAANRLENAVAAIIKEG
                     KHVTYDLKPDRNDPTAVGTREMAQAICARLK"
     gene            7018..8730
                     /locus_tag="VFB80_06240"
     CDS             7018..8730
                     /locus_tag="VFB80_06240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZN33398.1"
                     /translation="MPHAASPRQARPLRFESLESRQLLASVWQNPGQPLDVNRDLAVD
                     RRDVDTILAAHGRMYRPLGARPADSTQPYFDVSGDGKFDWTDYSRVNDAVVLKKPVVA
                     AQRTSAEAITGRVSNWTTSDRLQARLDGGAWRDVTPLIASKVFTLGPAELATVAAAPL
                     AAGQHVLDLRILRSGRTAGVGMDLAFEFLSPSGSGPQPEVDPTVVTQVPWTQAAPPGV
                     VIDFEPASTKRYVGSPSIVVLPSGEYVASHDLFGAGTVSDTTELFVSRDQGRSWQPLA
                     TLRGQFWSTLFVHSGQLYLLGTDRVNGAVVIRRSSDGGATWTTPSASTSGKLLAGQYS
                     TAPTPVIVSGGRIWRAVEAVRSGSWGLNFLPFVMSAPVDADLLNAASWTSSNQITPQS
                     TWLGGQFRGFLEGNAVPAPGGGIVNLLRVHTTTYPERAALVHISADGKTATFNPQKDF
                     VAFPGGTKKFTVRLDPVSGKYWALANDTPPEYRGLAAIERTRNTLSLISSPDLRTWRA
                     LRIVLQHANVAKSGFQYADWQFDGNDLIAAIRTAFQEPTGALAASGHDSNYLTFLRVK
                     NFRG"
     gene            8777..10198
                     /locus_tag="VFB80_06245"
     CDS             8777..10198
                     /locus_tag="VFB80_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN33399.1"
                     /translation="MGQSIAPPSTFRAMLALALPVLAEESLNLLVGYTDWFLAGRFLS
                     GREPLAAMGLMSYFLWMLPSYFTLVSIGALAVVARLVGAGQRQEAAHVARQALWLGLC
                     VAAIGILLALFLGGRFVAAMQLEGHSAELANRYMRILTPAIPFVMAEQVGTACLRGAG
                     DTVTGLWARVVLNVVNAIVSTTLVAGLGPFPQVGWDGLAIGTACGHAVGGSIILWRLF
                     RGGGGLQLVAAKPIERDHWLRLWKPDTATMRRILRVGVPGGIDVWSVLACHLIYVAII
                     NSLGTVAQAAHGLGVQIEAMSYLPGSAFQVAAATLAGQSLGAADQRRAVRGVLLCALS
                     ACTIMTAAGLILFFGGESIALVFLGQRGETSELTGRLLKIVAFSCPSLALLMVFLGAL
                     RGSGDTAWPLAITFIGLVGVRIPGACLLAWDELRVPLLGLALPAAGLGVAGAWYAMIA
                     DVILRSLLAAGRFFHGGWQKVRV"
     gene            complement(10207..10881)
                     /locus_tag="VFB80_06250"
     CDS             complement(10207..10881)
                     /locus_tag="VFB80_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HZN33400.1"
                     /translation="MEITEVRIKLMEDSDDRLQAFCSITFDNSFVVRDLKIIDGTNGP
                     FVAMPSRKLTSHCHQCGSKNHLKAQYCNQCGARLREDRTIRDAEGRAKLYADVAHPIN
                     SQCREMIQNRVIVEYRTELERAKQPGYASRYDEDYDDDVDAASLVAEERRQSVPQPSG
                     AHPQPPQPVPAPHIQQPPAPASAGDAPAGGPHFTAPPQRETVKETQNPPAKKEPPRGD
                     FGAGIF"
     gene            complement(10922..11776)
                     /gene="ispE"
                     /locus_tag="VFB80_06255"
     CDS             complement(10922..11776)
                     /gene="ispE"
                     /locus_tag="VFB80_06255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZN33401.1"
                     /translation="MTTPAKINLFLEILGRRGDGYHELQTLLAAISLYDTLHFMPDDS
                     GEIKLACRWAGKGSGEIPTGPENLVWRAAALLRERARTRAGAAIELIKRIPAAAGLGG
                     ASSDAAATLVAANLAWRLDWPRERLSELAAELGSDVPFFLKGGLALARGRGERLQSLP
                     PARLHIALVRPPVGLSTVDVYRACRPATEPSGAANLLAALARGDAAAAGRGLVNGLEP
                     AASGLTPWIGRLRREFERQDVWGHQMSGSGSSYFGLCRHARHARRVARRLRARGLGAT
                     CAATTAVA"
     gene            complement(12024..13325)
                     /locus_tag="VFB80_06260"
     CDS             complement(12024..13325)
                     /locus_tag="VFB80_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33402.1"
                     /translation="MLHASRVISLSVLLAAAICPAGRGEDWPQWRGPRRDGVWRESGL
                     VEKFASNSLTPKWRVPIGAGYCGPTVAQGRVYVMDRLTSPTSTERIHCFDEQTGKEIW
                     SLAYECPYKTVRYAAGPRASVSIDAGKAYALGSTGRLHVLDTATGKIVWQKDLDEEYR
                     IEMPIWGIAASPLIFENLLILHIGGEGACVVALNKETGAEVWRALSDRAQYSAPIVVQ
                     QAGQNVVICWTGDSVAGLAAASGKVLWRYPWKPKNMPIGVATPVVEGDRVFFTSFYDG
                     SLMLRLLPDRAEVEKVWQIVGRDEQNTLALHSIIATPVFENGYLYGTDSYGELRCLDA
                     RTGERVWENLTAVPKARWSTIHFVKNGERHFMFNERGELLIGKLSPRGFEEISRAKLL
                     EPTTDQLPQRQGVCWSHPAFANKHIFARNDKELVCASLAAE"
     gene            13533..14432
                     /locus_tag="VFB80_06265"
     CDS             13533..14432
                     /locus_tag="VFB80_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN33403.1"
                     /translation="MEIKLIDCHQHLWDLEKFKLPWIKPGTLLARSYVIRDYVAAIEG
                     TGIQHAVYMEVDVDPAQQQTEAEHLIEICKSGNAPTIGAVVSGRPASDGFAKYATQFK
                     ASPYVKGIRQVLHGGSTPAGFCLSESFVRGMQLLGELGLSFDLCLRPKELSDGARLAE
                     LCAGTRFIVDHCGNADPKAFFKSGDPRLAGLTPEHPADAWRRDIERLAAQKNVVCKIS
                     GIVARVTKEWSAADLAPIVNHCLDSFGPDRVIFGSDWPVCLNGAPLRDWASALKQIIA
                     SRPATEQRKLLFDNAVKLYRLTV"
     gene            complement(14439..14750)
                     /gene="rplU"
                     /locus_tag="VFB80_06270"
     CDS             complement(14439..14750)
                     /gene="rplU"
                     /locus_tag="VFB80_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZN33404.1"
                     /translation="MYAIIAADGRQYKVQSGDVIQIDLREGANPGDAVKFDNVLLVSG
                     EGGVKVGQPTVAGASVAAEVVSQDKGEKIYIQKMRRRKNFRKRTGHRQKYTRVKIGAI
                     S"
     gene            complement(14879..15304)
                     /locus_tag="VFB80_06275"
     CDS             complement(14879..15304)
                     /locus_tag="VFB80_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HZN33405.1"
                     /translation="MTQIVQNCLLVALGGALGTLARYGIGIAAAKLLGKGFPWGTLIV
                     NVGGCFAMGVVLGAIASWESQSPEAMTPDIRGRIELWRTFLAVGLLGGLTTFSSFGGD
                     TIRELQGGQPGIAVANVVSNVALSLVAVWCGLATMRMAD"
     gene            15376..>16588
                     /locus_tag="VFB80_06280"
     CDS             15376..>16588
                     /locus_tag="VFB80_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HZN33406.1"
                     /translation="MPVAAARSRIDTVYLHPDDNICVAARSLAAGDSLEIAGARFTLV
                     QPVRIGHKIAVRPIAKGEFVRKYGQIIGQATDSVPPGGWVHSHNLINGEFARDHAPSS
                     AVPPPPAPLEGRTFLGYKRASGKVGRRNYVAVISTVNCSATVSKYIAQRFDRDALRQF
                     PNIDGVIAIKHGGGCGIQYQGLQHEILNRTLAGMAKHANIGGYLLVGLGCEQATSGYL
                     MQSQNLVQIEGNGSVGNALRGVPPVLSMQDEGGSVKTIEAGVRLLGELLPRANDVKRV
                     PIPASELILGTNCGGSDGNSGVTCNPALGVASDMLVACGGTSALAETTEVYGAEHLLT
                     RRAVNPAVAEKLLARIKWWLWHTALYGVEIDNNPSIGNKEGGLTTIAEKSLGAVAKGG
                     QAALSAVYEYAE"
BASE COUNT         2705 a         5547 c         5499 g         2837 t
ORIGIN      
        1 gttctttgaa aattgaattt ccagcgccca agcctgcggc gtcgtcctcg cagcaacttt
       61 gctcgatggc atattggctg gtgtctgaaa actgtcgcaa attgttacgc aacaataact
      121 tgcgtcgagt ttttgccgcc ggacgttttt ctggcgtatg cgcgcatcgc gatgagccgc
      181 aaatttcgcg tccgcccagc gcgcataatg cgcataatgc ggcaactcag aattttgacg
      241 caagtgaagt cgcagtaata agttgcgtcg aaatccactg tcgaaggttt ttctgcgcgt
      301 atgcgcgcat tggagtcagg cagacgatcg cggctgtgtt tagccttgtg gaggcccgct
      361 tgccggaaac aaagtgaccg agtttcccga tacgcgcgac agcctgctga tgcaggtccg
      421 ctcgcccgcg aatcgcgagg cgtgggacca gttcgccctg atctacaggc cggtgatcta
      481 ccgcctggct cgccggcggg gacttcagga cgcggacgcc caggatctgg ctcagcaggt
      541 gctcgtgtcg gtcgcctcgg ccatcggccg ctatgagaag tcgggcgagt cggtgcggtt
      601 ccgccactgg cttcgtaccg tgacccgcaa cgcgatcgtc aatgccctct cgcggcagcc
      661 gcacgaccgc gcggcgggcg gctcgtcggc cgcggactgg ctggtggaac agcccgtcgc
      721 tgatcccgag gcggatgccc aggtcgaatt ggaatatcgc cgggagttgt atctgcgcgc
      781 cggccgcatc gtccgccgcg acgtcgagcc ggacacctgg cgggcgttcg agctgacggt
      841 gatcgaaaac gagagcgccg gcgaggcggc cgcccgcctg cagatgcagg tcgggtcgat
      901 ctacgcggcc aagagccgca tcatgcggag gctgcgcgaa gtggtgaaag aactggagca
      961 gtgggaaaaa tgaaactgaa cttgccagct agcggctgcg acgcggagcg gcttgaagcg
     1021 ctgctctgcg gccgtttgag cgacgccgag cagcgcgaag caatcgcaca tttgaacgac
     1081 tgcggttctt gccggcagcg cctgcaggcg caggccgccg agcccgaggc ctggagcgaa
     1141 gccgagagcc tgctgcggcc gtgcgagttc gctgcgcccg ccgccggaga agctcgcacg
     1201 ccgccagcga ttcaacacgt cctggatgag ctcggggcca ccgacgatcc gcagatgctg
     1261 ggacgcctcg gcggctatga agtctcgggc gtcgtcggcg ctggcgggat gggcgtggtg
     1321 ctcaaggcga tcgacaagtc gctcgatcgc acggtagcga tcaaggtgct cgcgccgcac
     1381 ctggcgacca gcggcgcggc tcgccggcgg tttgcccgcg aagccaaggc ggccgcggcg
     1441 gtcctgcatc ccaacgtgat cgcgatccac agcgtctcga acgacgaatc gctcccctac
     1501 ctggtgatgc cgtacctgcg cggccaatcg ctgcagaagc ggctcgatgc cgagggcccg
     1561 ctcccagtcc aggaagtgct gcggatcggc gcgcagatcg ccgccggact ggccgcggcc
     1621 cacgcccagg gcctggtgca tcgcgacatc aagcccgcca acatcctgct cgaagaaggc
     1681 gtggagcggg tcacgatcac cgatttcggc ctcgcccggg cgagcgacga cgcctcgatc
     1741 acgcacagcg gcctgatcgc cggcacgccg caattcatgt cgcccgagca ggcccgcggc
     1801 gacgcggtgg accagcgcag cgacctgttc agcctcggca gcgtgctgta tgcggcctgc
     1861 accggccgcc cgcccttccg cgccgagacg agctacggcg tgttgcggcg aatcacagac
     1921 gacgagccca cgccgatccg cgacatcaac ccggatattc ccgattggct gtgcacggtc
     1981 gccagccggc tgatgtcgaa gcggccccag gagcgttttg agtcggccca ggaagtcgct
     2041 aacctgctgg agcagtgcct ggctcacgtg cagcagccga cggcggtgcc gctgccgcag
     2101 cgcctcgtgt ctcgtccgcc cagcgcgaat tccgccacgc cgctggcgac cactttccgc
     2161 tacggagtac tggccatgat tgccgctctg ggactgggac tgctcgccgc tttcgtctgg
     2221 ctggcggctg atccgccgga aatctccggc cagtggagcg gcgacgagtg gggccaggtc
     2281 gtcctcaaaa agctcgagag cggcgcctat gaaggcactt acaccgacac gttcggcaaa
     2341 gaggccggcc agctggagct gaagtggtcc gccgtggagc gccgcttcaa gggcgcctgg
     2401 cgcgaaggcc aggaccgctc gggcaaattg gccgtccgcc tcgtcgatgg cgaaatccgc
     2461 ggcgcatgga cggccgacaa ggagtcgcgc atcgggcccg gcacgccgga gctggccgat
     2521 ctgctctgga aacgcagtag cggcatttcg accacgctgc ctccggctgc cgtggccggc
     2581 aaggccagcg cagagccgag ggaaaggtcc acgatcatgc cggggcgact gaactacttc
     2641 accaccggcc acagcatcaa ggtcgcctgc tcggcagacg gcaagctgat cgccgtggcc
     2701 aacggcaacc cgacgcggat tctgcaagtc ggcggaatca gccgcgtcaa aggcgactgg
     2761 aagccgacgg ccgacatcct cgatctggag acgggcaagg tcgtcgtttc gctgcagctc
     2821 gcgaccagcg aggaacaggc ggtgctcgcc gccaccgagc gcaacgctca tctcgaagcg
     2881 acggcgctgg cgttttcgcc gaccgggaag atcgtggccg tcgggaccag catcggccag
     2941 gtgaagttct ttgacgcccg caccggcgag ctgctgcgct cgctggacca cgccgcggcg
     3001 aaactggccg acaaagagac tccggatagc tggcggtcga ttgcgcgggc gatgggcagc
     3061 gtcacctcgc tcgcgttctc gcccgacggc agcctgctgg cggtctgcgg ccagccgttt
     3121 cgccagtttt cggatgcgtt cgatggcgta tcgcggctgg gccgggagat tacgggcccc
     3181 gaccggctga aggtgtggga agtcgagacc ggcaagctca agcacgatct ggtcgggcac
     3241 acctatcccg acgaagtcgc cttttccacc gacggcaccc tgctggccag cgcgggacgc
     3301 tggtcgagcg agggcgacga cggcagcggc gtgatcttct ggaatccgca gaccggggcg
     3361 aaagtgggca ggctcaaggc cgacgccaac gtcatcacgc aggccgtggc cttctcaccc
     3421 ggcggcaaac tggtggccgt cggctcgcag acctacagca gcgcgaccaa tgacgacaac
     3481 acgacgacga tcagcctcgc cagcgcgccg acggggctcg tgcattggaa gcatacggtc
     3541 gccggctggg cgaatcccaa agccttttcg ccgcatggcg tcgtcgtctt gtgcaagaaa
     3601 tcgatcctgc tgctggatgc cgagaccgga caggtgaaac gcgagatcaa ggcgacggat
     3661 ctgtccaagg aggggcggtg ggacgatctg gccgtcgccg gccggtggct ggtgatcgcg
     3721 tcggccgacg agtacgaagg gaacgtcgag ctaatggagc tcgccggtcc agacgctgct
     3781 gccgccgctc gtcccgcgcc gcagccgaaa gcggaggggg ctgtgctcag acaagtgcgg
     3841 cggtttcaga cggccggcag catccagtcc gtcgcggttt caccggacgg caagcggatc
     3901 gccgttgccg gcggcgaaga gtcggctccc ccaaactttg actggaaacg gcaggtggag
     3961 attctcgacg ccgatacggg gcaaagcgtc gtcaaaatcc gcctttcgac caaagaagaa
     4021 gacgcggtgc tggccgcgac cgcaggatcg tatcatttcg aggttctcgc gttctcaccg
     4081 gatgggagcg tgctggcggt cgggacgagc gcaggccagg tcaagctgtt taacccccgc
     4141 accggcgaac ttgtgcggtc gctcgacgac gaaccggcca aacgcgccga caaggagacg
     4201 ccagagaaat acaagtcgct ggcccgcgcg ctgggaagcg tcaattcatt ggctttttct
     4261 cccgacggca gcctgctggc agtctgcggc agctcgttcg aggacaaacc tatcgtcgat
     4321 gaccggctcg agcgcacgcg actctccgtc gccggccccg gcaggctgaa ggtctgggac
     4381 gtcaaaacgg gggcgctcaa gcacgacctg gccgggcacg acgagcacgc caatgccgtt
     4441 gccttttcgg ccgacggcag cttgctggcc agcgccgggc gcgggatcgg cgcgagcgga
     4501 cacgaatctg gcgtaatcgt ctgggatgtc gcgtcgggaa agccgcggag cacgatcttg
     4561 atcccggcca acggcggcac gtacgcggtc gccttctcgc cgacgaaaaa gctgctggcg
     4621 atcggctcgc gaaacttcga caaggagaac gacacccgcc gggcgacggt cagcgtggcc
     4681 tacgcgctgt cgggcgtgat ggaatggcag cagaccttca gtgtctacgc caatcccaag
     4741 gccttcctgc ccgacggcaa aagcgtggcg gtgatgtgcg gtcgagaagc gatccgcttc
     4801 ttcgatgccg agtcggggac cgtgaagtac gagatcaagg cagcccacgc ggtacggccg
     4861 gcggggtgga acgatttcgc cgtcgcgccg cagggccgcc tgctcgtgat cggcggcgag
     4921 gaccgcgacc acaagggcat cgttgagatt tgggacctcc ccgccccggc ggccgatccg
     4981 ccagcgcggg cagactcacc tggcggactt tcgccgcgct gacagccgcc gcttcaccga
     5041 cgggctgttc agctcgcgga gcgcgtcctt gcccacccac cgcggcgtga cgcggtcgga
     5101 agcggcgagg cgctcggcga cggccaccgc ggccgcgtgg agcgcgtcgc cgcgcttgcc
     5161 gatcgcccgg agggccatgt tgacggcctt cttgacgaag ttgcggtcgt cgtccgcggc
     5221 cacttcgatc agccccagac tttgatggaa cggctcgtca cccgaacgtt tgtcgtgggc
     5281 ggccagggcc cacagcaggg cgaaggcggc ccgcttctgg aactcgcccc gctggccggc
     5341 ccaggccttg accttccgcc aggcgtgcgg cgtcttgtcg aacaaatgaa aacagagcgc
     5401 gtcgcaaatc gcccagttgt cgaagtttcg ctgccagtgg tccatctgga ccggcgtcac
     5461 ctgcaggggg tcgtcgataa acgccgtcag catccgcgcc tcgtaccagc ccgtctccca
     5521 caggctgacc gccagcgcgt gattttgccc cagccgccgc gcgagctttc gcagatccgc
     5581 cacggagacg ccaaacgcct tgtccgacgg aatggcgaac cgcgccatgc cgtccttgac
     5641 tttctggctg cccattcgct ggagctcggc cacgacggca gcggcgtccg ccatcgcggg
     5701 cgatttggtt ttcccgcccc gctgcacggt ttcgtcgatc gtcttggtct tcgtgggcaa
     5761 gcgttatttc agccgggcgc agatcgcctg tgccatttcg cgggtgccga cggccgtcgg
     5821 gtcgttgcga tcgggcttga ggtcgtaggt gacatgcttg ccttccttga tgatcgcggc
     5881 cacggcgttc tccaggcggt tggcggcgtc catctcgccc aagtggcgga gcatcaacat
     5941 gccgctaagg atcagcgcgg tcgggttgac cttgttctgg cccttgtatt tcggggcgct
     6001 gccgtgggtg gcttcgaaga ccgcgccgcc ggggccgatg ttctcgccgg gcgcgacgcc
     6061 caggccgccg accaggccgg cgcccagatc gctgacgatg tcgccgtaca gattcggcag
     6121 caccagcacg tcgtacagct ctggcttctg cacgagctgc atgcacatgt tatcgacgat
     6181 ccgctcctcg tatttgatgt cgggatattc cttggcgacg gcggtcgcgg tgtgcaggaa
     6241 caggccatcg gagtatttca tgatgttggc cttgtgcaca gccgtgacgc ttttgcgctt
     6301 gttctggcgg gcatagtcga acgcgcagcg gacgatccgc tcggtgccgc ttcggctgat
     6361 cggcttgatg ctgacgcccg tctcggcggc tttggtcttg atcttcttgt cggtcgagat
     6421 tttgttaatg aaatcgatca ggtcggcggt cgccggcttg cccgcctcga actcgacgcc
     6481 ggcatacagg tcttcggtgt tctcgcggac gatgacgata tcgacgccga tctcgctgaa
     6541 gtagctccgc acgccggggt agtacttgca cggccggatg caggcgaaca ggcccagctc
     6601 ctgccgcagg tggacgttga tcgagcgaaa gccggtgccg acgggcgtgg tgatcggggc
     6661 tttgagggcg cacttcgtgc ggcggacgct ggccagcacg ctgtcgggca gcggcgtgcc
     6721 ggtcttggcc atcacatcga cgccggcttc ctgcacgtcc cagttgatct tcacgccggt
     6781 cgcgtcgagg cacattcggg cggcctgcga aagctcgggc ccggtgccgt cgcccgtaat
     6841 cagcgtcact tcgtgagcca tcgccaaaac tccttaaatc agcaagggat acggcgggca
     6901 aactaacacg caaatccgga accgtcaacg caacaccaag ttcatggttc aaggttcaag
     6961 gtggattgcc gctttgaacc ttgaactgtg ttttgagccg actacgctcg aatgggaatg
     7021 ccccacgccg cttccccgcg ccaagcccgc ccgcttcgct tcgagtcttt ggagagccgc
     7081 cagctgctgg cttcggtctg gcagaacccc ggtcagccgc tggacgtgaa tcgcgacctg
     7141 gcggtcgatc gccgcgacgt ggatacgatc ctcgccgcgc acgggcggat gtaccggccg
     7201 ctcggtgcgc ggcccgccga ttcaacccag ccgtacttcg acgtcagcgg cgacggcaag
     7261 ttcgactgga ccgactattc ccgcgtcaac gacgccgtcg ttttgaagaa gccggtcgtc
     7321 gcggcccagc gcactagcgc cgaagcaatc accggccgcg tttcgaactg gacaacgagc
     7381 gaccgcctgc aggcccgcct cgacggcggc gcgtggcgcg acgtcacacc gctcatcgcc
     7441 agcaaggtct tcacgctcgg cccggccgag ctcgccacgg tcgccgccgc accgctggcc
     7501 gcggggcagc acgtgctcga cctgcggatc ctccgcagcg gccggacggc cggcgtcggc
     7561 atggacctgg ccttcgagtt tttatcgccc agcggcagcg gaccacagcc ggaggtcgat
     7621 ccgaccgtcg tcacgcaagt gccctggacg caagccgcgc cgccgggcgt ggtgatcgac
     7681 ttcgagccgg ccagcaccaa gcgctacgtc ggctcgccga gcatcgtcgt cctgcccagc
     7741 ggcgagtacg tcgcctcgca cgacttgttc ggtgcgggca cggtcagcga cacgaccgaa
     7801 ctgttcgttt cgcgcgacca gggccgcagc tggcagccgc tggcgacgct tcgcgggcag
     7861 ttctggtcca cgctgttcgt ccatagcggg cagctctacc tgctgggcac cgatcgggtc
     7921 aacggcgcgg tcgtgatccg ccgctccagc gacggcggcg cgacctggac gacgccatcg
     7981 gcgagcacca gcggcaagct gctcgcgggg cagtattcca ccgcgccgac gccggtgatc
     8041 gtttccggcg gccgcatttg gcgggccgtc gaggccgtgc gttccggcag ctgggggctc
     8101 aatttcctcc cgttcgtcat gtcggccccg gtcgatgcgg acctgctgaa tgcggccagc
     8161 tggacctcgt ccaatcaaat cacgccgcag tccacctggc tcggcgggca attccgtggc
     8221 ttcctggaag gcaacgccgt gcccgcgccc ggcggcggga tcgtcaacct gctccgcgtc
     8281 cacaccacga cctaccccga gcgggccgcg ctcgtccaca tctcggccga cggcaaaacg
     8341 gcaacgttca acccgcagaa agacttcgtc gcgtttccgg gcggcacgaa gaaattcacc
     8401 gtgcggctgg atcccgtcag cgggaaatac tgggccctgg ccaacgacac gccgcccgag
     8461 taccgcggcc tggcggcgat cgagcggacc cgcaacacgc tgtcgctcat ctcttccccc
     8521 gacctccgca cgtggcgcgc cctgcggatc gtcctccagc acgccaatgt cgccaagagc
     8581 ggctttcaat acgccgactg gcagttcgac ggcaatgacc tgatcgccgc catccgcacg
     8641 gcctttcaag agccgaccgg cgccctcgcc gccagcggcc acgactcgaa ctatctgacg
     8701 ttcttgcggg tgaagaactt ccgcggctaa ctcttggccg ccatactgga cggctttcgt
     8761 cgccggggtc gcaccgatgg gccagagtat cgcgccgccg agcacttttc gggcgatgct
     8821 cgcgctggcc ttgccggtgc tagccgagga gtcgctgaat ctactcgtgg gctacaccga
     8881 ctggttcctg gccgggcggt ttctcagcgg ccgcgagccg ctggcggcga tggggctgat
     8941 gtcctatttc ctgtggatgc tgccgagcta cttcacgctc gtcagcatcg gggcgctggc
     9001 cgtcgtggcc cgcctggtcg gcgcgggcca gcggcaggag gcggcgcacg tcgcgcggca
     9061 ggctctctgg ttggggctgt gcgtcgctgc catcggcatc ctgctggcgc ttttcctcgg
     9121 cggacgattc gtcgcggcca tgcagctcga aggacactcc gcggaactcg ccaaccgcta
     9181 catgcgaatc ctcacgccgg cgatcccctt cgtgatggcc gagcaggtcg gcactgcctg
     9241 cctccgtggc gcgggcgata ccgtcaccgg cctgtgggcc cgcgtcgtcc tcaacgtggt
     9301 gaacgcgatc gtcagcacga cgctggtcgc gggtctcggt ccatttccac aggtcggctg
     9361 ggacggcctg gcgatcggca ccgcctgcgg gcacgcggtc ggcggctcga tcatcttgtg
     9421 gaggctgttt cgcggcggag gaggtctgca acttgtggcg gcaaagccaa tagagcgcga
     9481 ccactggttg cggctttgga agccggacac ggccacgatg cggcggatcc tgcgggtcgg
     9541 cgtgcccggc ggcatcgacg tctggtcggt gctggcctgc cacctgatct acgtggcgat
     9601 catcaacagt ctcggcaccg tcgcgcaggc ggcccacggg ctgggcgtgc agatcgaagc
     9661 catgtcgtac ctgcccggat cggcatttca ggttgcggct gcgacgctcg ccgggcaatc
     9721 gctcggcgcg gccgaccagc ggcgggcggt gcgcggtgtc ttgctctgcg cgctctcggc
     9781 ctgcacgatt atgaccgccg ccgggctgat tctcttcttc ggcggcgaat cgatcgcgct
     9841 ggtgttcctg ggccagcgcg gcgagacctc ggagctgacg ggccggctgc tcaagatcgt
     9901 cgcgttctcc tgcccgtcgc tggcgctgct catggtcttt ctgggcgcgc tgcgcggcag
     9961 cggcgacacg gcctggccgc tggcgatcac gttcatcggc ctggtcggcg tgcggattcc
    10021 cggcgcgtgc ctgctggctt gggacgagct gcgcgtgccg ctgctcggcc tggccttgcc
    10081 cgctgccggg ctgggcgtcg ccggcgcgtg gtacgcaatg atcgccgacg tgatcctccg
    10141 cagcctcctc gccgccggtc gcttcttcca cggcggctgg caaaaggtgc gggtctagct
    10201 tctcagctaa aaaatccccg ccccaaaatc gccccgcggc ggttccttct tggcgggcgg
    10261 attctgtgtt tccttgaccg tctcgcgctg cggcggcgcg gtgaagtgcg ggccgccggc
    10321 cggcgcgtca ccggcggacg cgggcgccgg aggctgctga atgtgtggcg caggcacggg
    10381 ctgcggcggc tgcgggtgag cgccgctggg ctgcggcacc gactgccggc gctcctccgc
    10441 caccaggctg gcagcgtcca cgtcgtcgtc gtaatcctcg tcatagcgcg aggcatatcc
    10501 cggctgcttg gctcgttcca gctccgtgcg gtattcgacg atcacgcgat tctggatcat
    10561 ctcgcggcac tgcgagttga tcgggtgggc gacgtcggcg tagagcttgg cccgcccttc
    10621 agcgtcgcgg attgtgcggt cttcgcggag gcgggccccg cactggttgc aatactgggc
    10681 cttgaggtgg ttcttcgagc cgcactgatg gcagtggctg gtgagcttgc ggctgggcat
    10741 cgcgacgaac gggccgttgg tgccgtcgat gatcttcagg tcgcggacca cgaagctgtt
    10801 gtcgaaggtg atcgagcaga aggcttgcag ccggtcgtcc gagtcctcca tgagcttgat
    10861 gcggacctcg gtgatttcca tgacgaatcc ctccgtagtc gtgttctcgt ccgttctggg
    10921 gtcaggccac cgccgttgtg gccgcgcagg ttgctcctaa tcctctcgcc cgcaagcggc
    10981 gcgccacgcg ccgggcgtgc cgggcgtggc gacacagtcc gaagtagctc gagccgctgc
    11041 cgctcatctg gtgtccccac acgtcttgcc gctcgaattc gcgccgcagg cggccgatcc
    11101 agggggtcaa cccgctcgcc gccggctcca gtccgttgac gaggccgcgg ccggccgccg
    11161 ccgcatcgcc cctggccagc gccgcgagca ggttcgccgc gccgctcggc tcggtcgccg
    11221 gccggcaagc gcgatagacg tcgaccgtac tcaatcctac cggcgggcgg accaatgcga
    11281 tatgcagccg cgccggcggc agcgattgca gccgctcgcc gcggccgcgg gccagcgcca
    11341 gtccgccttt caggaagaac ggcacgtcgc tgccgagctc ggcggccagc tcgctcaatc
    11401 gctcgcgcgg ccaatcgagc cgccacgcca ggttggctgc gaccagcgtg gccgccgcgt
    11461 cactcgacgc gccgcccaac ccggccgcgg cgggaatgcg cttgatcaat tcgatcgccg
    11521 cgccggcccg ggtgcgggct cgctcgcgaa gcagcgccgc ggcccgccag acgaggttct
    11581 cgggccccgt cgggatctcg ccggaccctt tgccggccca gcggcacgcg agcttgatct
    11641 cgccgctatc gtcgggcata aagtggagcg tgtcgtagag cgaaatcgcc gccagcagcg
    11701 tctgcagctc gtgatagccg tcgccccgcc gacccaagat ctccaagaac aagttgatct
    11761 tggcgggcgt cgtcacttca atttgtgatc cgagtcggcg taaatgcatc cttcgccgct
    11821 gcgtccggcc gggcgagagc ccgccgctgc gcaacacctc ttcgaatcaa ggttcgttcc
    11881 agccggtccg ccagagagag aagtctcgtc acagcagata cgccaaatgg cggaccgaca
    11941 ctcccgattg taccggcatg ctcaggtggg tcaatcttga tgtacgctgc gtccgctgcc
    12001 gtaccacgcg ccgttgcgcg tcgctactcg gccgccaggc tcgcgcagac cagttccttg
    12061 tcgttccgcg cgaaaatgtg cttgttcgcg aacgccgggt gcgaccagca cacgccctgc
    12121 cgctgcggca gctggtcggt cgtcggctcc agcagcttgg cccggctgat ctcttcgaag
    12181 ccgcgcggcg agagcttgcc gatcagcaat tcgccgcgct cgttgaacat gaagtgccgc
    12241 tcgccgttct tcacgaagtg gatcgtgctc cagcgggctt tgggcaccgc ggtgagattc
    12301 tcccacacgc gctcgcccgt ccgggcatcg aggcagcgca gctcgccgta gctgtcggtg
    12361 ccgtagagat agccgttttc gaacaccggc gtggcgataa tcgagtgcag ggcgagcgtg
    12421 ttctgctcgt cgcggccgac gatctgccag accttctcca cctcggccct gtccggcagc
    12481 agccgcagca tcagcgagcc gtcgtagaac gaggtgaaga agacgcggtc gccctcgacg
    12541 accggcgtgg caactccgat cggcatgttc ttcggcttcc agggatagcg ccacaggacc
    12601 ttgccgctgg cggccgccag tccggccacg ctgtcgccgg tccagcaaat cacgacgttt
    12661 tggccggctt gctgcacgac gatcggagcg ctgtactggg cccggtcgga aagtgctcgc
    12721 cagacttccg cgccggtctc cttattcagg gcgacgacgc acgccccttc gccgccaatg
    12781 tgcaggatga gcaggttctc gaagatgagc ggcgaggccg cgatgcccca gatcggcatc
    12841 tcgatgcgat attcctcgtc caggtctttc tgccacacga ttttgcccgt ggcggtgtcg
    12901 aggacgtgca gccggccggt cgagcccagg gcataggcct tgccggcatc gatcgagacc
    12961 gaggcccgcg gcccggcggc atagcggacc gtcttgtacg ggcactcgta ggccagcgac
    13021 cagatttcct tgccggtctg ctcatcgaag caatggattc gctcggtgga agtcggcgac
    13081 gtgaggcgat ccatgacata gacgcggccc tgcgcgacgg tcggcccgca ataccccgcg
    13141 ccgatcggca cgcgccactt cggcgtcagg ctgtttgagg caaacttctc gaccaggccg
    13201 ctttcgcgcc acacgccgtc gcggcgcggc ccgcgccact gcggccagtc ctcgccgcgg
    13261 cccgctgggc agatcgccgc ggccaaaagc acggatagcg aaatcacccg ggatgcgtgc
    13321 aacatgcgcg gcctttcttt aggatcatgt cgtttaaccc ggagccggag gcgacggcgg
    13381 ctactcgcga aaagccgtcg cctgcggctc cgagttaaac gaaggggcaa caataagcta
    13441 tgaacaccag ccgccgacaa ttctcagcgc aagtcgccgc cgccgcaacc gcagcgctgg
    13501 cctgtccgcc tgccttcgcc caggaccctg ccgtggaaat taagctgatc gattgccacc
    13561 agcacctttg ggacctggag aagttcaagc tgccgtggat caagccaggc acgctgctgg
    13621 cccgcagcta tgtgatccgc gactacgtgg ccgcgatcga gggcacgggc atccagcacg
    13681 cggtgtatat ggaggtggac gtcgatccgg cccagcagca gaccgaggcg gagcacctga
    13741 tcgaaatctg caagagcggc aatgcgccga cgatcggcgc ggtcgtttcc gggcggccgg
    13801 cgtcggacgg ctttgccaaa tacgcgacgc agttcaaagc gagcccctac gtgaagggca
    13861 tccgccaggt gctgcacggc ggctcgactc cggcgggttt ctgcctgagc gagagtttcg
    13921 tccgcggcat gcagctgctg ggcgagctcg gcctgagctt cgatctctgc ctgcggccga
    13981 aggaattgag cgacggcgcg cggctggccg agctctgcgc aggcacgcgg ttcatcgtcg
    14041 atcactgcgg caacgccgac cccaaggcgt ttttcaagtc cggcgatccg cggctggccg
    14101 gactgacgcc cgagcatccg gccgacgcct ggcggcgcga catcgagcgg ctggcggccc
    14161 agaaaaacgt cgtctgcaag atcagcggta tcgtggcccg cgtgacgaaa gaatggtcgg
    14221 cggcggacct ggccccgatc gtgaatcact gcctcgactc gttcgggccc gaccgtgtga
    14281 tttttggcag cgactggccc gtctgcctga acggcgcgcc gctgcgcgac tgggccagcg
    14341 ccctcaagca aatcatcgct tcccgtcccg cgaccgagca gcggaagctg ctcttcgaca
    14401 acgccgtgaa actctaccga ctgaccgttt agcggaggct acgagatcgc gccgatcttg
    14461 acgcgggtgt atttctggcg gtggccggtg cgcttgcgaa aattcttgcg gcggcgcatt
    14521 ttctggatat agatcttctc gcccttgtcc tgcgagacga cctccgccgc aacgctggct
    14581 cccgccaccg tcggctgccc gaccttcacg ccgccttcgc ccgaaacgag cagcacattg
    14641 tcgaacttca cggcgtcgcc cgggttggcg ccttcgcgca agtcgatctg aatcacgtcg
    14701 cccgactgca ctttgtactg gcgtccgtcg gcggcaataa tggcgtacat gcttggcagc
    14761 tcacgactta gggcgatctt ctgacgcagc cgaactcgcc cgggctcgga cttggcacgg
    14821 agtgaaacat cgcaatatag ggtccggtcc accgagtgtc gaggggcgag cggccgaact
    14881 aatcggccat tcgcatcgtc gccaggccgc accaaaccgc gaccagagag agcgccacgt
    14941 tcgagacgac gttggccacc gcaatccctg gttgcccgcc ttgcagctcg cggatcgtgt
    15001 cgccgccgaa gctggaaaac gtagtcaggc cgcccaacag accgacggct aggaaggtcc
    15061 gccacaattc gatcctgccg cgaatgtccg gcgtcattgc ttctggcgac tggctttccc
    15121 agctcgcaat cgctcccagc accacgccca tggcaaagca gccgccgaca ttcacgatca
    15181 acgttcccca cgggaatccc ttgccgagca gctttgcggc ggcaatcccg attccatacc
    15241 gggccagcgt tcccaaggcg ccgccgagcg ccacgagaag acagttttga acgatttgcg
    15301 tcatgccggc gattgtaccc gcgcacgaaa aacggctgcc agatatgctg aagctatgcc
    15361 ttctgtgagc attttatgcc ggtagctgct gcccgctctc gcatcgatac cgtctatctg
    15421 catccagatg acaacatctg cgtcgccgcg cggagtctgg cggcagggga ttcgctcgag
    15481 attgcgggcg cgaggttcac gctggtccag cccgtgcgga tcgggcacaa gatcgcggtg
    15541 cggccgatcg ccaagggcga gttcgtccgc aagtacgggc agatcatcgg gcaggcgacc
    15601 gacagcgtgc cgcccggcgg ctgggtccac tcgcacaatc tgatcaacgg cgagtttgcc
    15661 cgcgaccacg cgccgtcgtc ggccgtgccg ccgccgcccg cgccgctcga gggccgaacg
    15721 tttctgggct acaagcgagc tagcggcaag gtgggcaggc ggaattatgt ggcggtcatt
    15781 tcgaccgtga attgctcggc gacagtcagc aagtacatcg cccagcggtt tgatcgcgat
    15841 gcgctgcggc agtttcccaa catcgacggc gtgatcgcca tcaaacacgg cggcggctgc
    15901 ggcatccagt atcagggcct gcagcacgaa atcctcaacc gcacgctggc cggcatggcc
    15961 aagcacgcca acatcggcgg ctacctgctc gtcggcctcg gctgcgagca ggccaccagc
    16021 ggctacctga tgcagtcgca gaacctggtg caaatcgaag gcaacggctc tgtagggaat
    16081 gccctccgtg gcgttccccc ggtcctctcg atgcaggacg aaggcggctc cgtcaaaacg
    16141 atcgaagccg gcgtgcggct gctgggcgaa ttgctgccgc gggccaatga cgtcaagcgc
    16201 gtgccgattc cggcgagcga gctgatcctg ggcaccaact gcggcgggtc ggacggcaac
    16261 agcggcgtga cctgcaaccc ggccctgggc gtcgcgtccg acatgctggt ggcctgcggc
    16321 ggcacgtcgg ccctggccga gacgaccgag gtctatgggg ccgagcacct gctgacgcgg
    16381 cgagccgtca acccggccgt cgccgagaag ctgctggcgc ggatcaagtg gtggctctgg
    16441 catacggccc tgtacggcgt cgagatcgac aacaacccgt cgatcgggaa caaggagggc
    16501 ggcctaacga cgatcgccga aaagtcgctc ggcgccgtcg ccaagggcgg ccaggctgcg
    16561 ctgtccgccg tctatgaata cgccgagc
//
LOCUS       DASRJB010000226         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4015690, whole genome shotgun sequence.
ACCESSION   DASRJB010000226 DASRJB010000000
VERSION     DASRJB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4015690"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFB80_06285"
     CDS             complement(<1..385)
                     /locus_tag="VFB80_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN33407.1"
                     /translation="MKRIGWILLLVAWTGPLAAEDFALHTFGRQQLTDVYYSEGIAAG
                     DLNRDGQVDIVYGPHWYAGPKFAEKREIFPARPQDRERYANHFFAWIYDFTGDGWNDV
                     LTAGFPGTPAFVYVNPRAEAFDKPWP"
     gene            complement(398..1351)
                     /locus_tag="VFB80_06290"
     CDS             complement(398..1351)
                     /locus_tag="VFB80_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33408.1"
                     /translation="MVRIAPFVLCLACASVAAQEQFAAKVTHTYKTAGDVEIKADVLG
                     FADDRTRPVVVWIHGGALIMGHRESVPRWLQDAARDNGFVLVSIDYRLAPETKLPEII
                     GDLESAFRWVREQGPKLFHGDAARVGVVGGSAGGYLTLTSGFRVQPRPAVLVSLWGYG
                     DLVGPWYSTPSPHPRHQTSKLTRVEAFQQVAGPPISDARQRKGDGGAFYQFCRQQGYW
                     PEAVSGWNPHTQAEKFHPFMPLVNVTPEYPPTLLIHGETDTDVPHEQSVQMAAALKKH
                     GVEHKLLSIPGAEHGLAGVPRETSDEAYRSAVDFLKAHLSK"
     gene            1849..2253
                     /locus_tag="VFB80_06295"
     CDS             1849..2253
                     /locus_tag="VFB80_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZN33409.1"
                     /translation="MPVQMQLTRIIISEINPQQVIYLREVEGDRTFPILIGLFEAESI
                     NTGVKRVQSPRPRTHELLVQSIEALGGELDSVVISELRDHIYFAKLRVKREGELVEID
                     SRPSDAIAVAVACDPILPIYVAEEVLEESTGN"
     gene            2418..>3003
                     /locus_tag="VFB80_06300"
     CDS             2418..>3003
                     /locus_tag="VFB80_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33410.1"
                     /translation="MARKTSRRKFLGTSAAIGAGFWVAGGIAPRESRAAIDKIHFASV
                     GVGGKGAGDSQDAGKAGDMVAICDIDDKNLNFAANRWPNAKKYHDFRKMFEEMEKSID
                     AVTVSTPDHCHAVIGAAAISRGKHCFIQKPLAKSIHEVRHLQRIATEKKVATQMGNQF
                     TADSNLRRAAQVIKDGHVGTVKEVHVWTNRPVWPQ"
BASE COUNT          569 a          979 c          970 g          485 t
ORIGIN      
        1 tgggccacgg tttgtcgaac gcctcggccc gcggattgac atagacgaag gccggcgttc
       61 cgggaaagcc ggccgtcagt acgtcgttcc acccgtcgcc ggtgaagtca tagatccagg
      121 caaagaagtg attggcatac cgctcgcggt cctgcggccg ggccgggaag atctcgcgct
      181 tctcggcaaa cttcggtccg gcataccagt gcgggccata cacgatatcc acctggccgt
      241 cgcgattgag gtcgcccgcc gcgatgccct ccgagtaata cacgtcagtc aattgctggc
      301 ggccaaaggt gtgcagggca aagtcctctg ccgcgagcgg gccggtccaa gcgaccagca
      361 acaagatcca gccaatgcgt ttcatggagc gactccgcta tttgctcaaa tgcgctttca
      421 ggaaatcgac tgccgagcga taggcctcgt cgcttgtctc ccgcgggacg ccggccaggc
      481 cgtgctccgc accgggaatg ctcagcagct tgtgctcgac tccgtgcttc ttcagcgcgg
      541 cggccatctg cacggattgt tcgtgcggca cgtcggtgtc cgtttcgccg tggatcaaca
      601 gcgtcggcgg atactcgggc gtgacgttca ccagcggcat gaagggatgg aacttctcgg
      661 cctgcgtgtg cggattccag cccgaaaccg cctccggcca gtatccctgc tggcggcaga
      721 actgatagaa cgcgccgccg tcgcccttcc gctgccgcgc gtcggagatt ggcggcccgg
      781 cgacctgctg gaaagcttcc acgcgcgtga gcttgctggt ctggtgccgc ggatgcgggc
      841 tgggtgtgct gtaccacgga ccgacgaggt cgccgtagcc ccacagcgaa acgagcaccg
      901 ccggccgcgg ctgcacgcga aatccggaag tcagcgtcag gtagccaccc gcggagccgc
      961 cgaccacgcc gacccgcgca gcgtcgccat gaaacaattt tggcccctgc tcgcggaccc
     1021 agcgaaaggc gctctccagg tcgccgatga tctccggcag cttcgtctcc ggcgcgaggc
     1081 ggtaatcgat cgacaccagc acgaagccgt tatcgcgggc ggcgtcttgc agccaccgcg
     1141 ggacgctttc gcggtggccc atgatgagcg ctcctccgtg aatccagacg acgaccggcc
     1201 gcgtgcggtc gtcggcaaag cccagcacgt cggccttgat ctccacgtcg ccggctgttt
     1261 tgtaggtgtg cgtcactttg gcggcgaatt gttcctgggc tgcaaccgag gcacacgcca
     1321 ggcagagcac gaaaggggcg atgcggacca tgatcctcct cctgcggctt caatgtacaa
     1381 gggccgcgtc cgtcaagcga aaaggaatcc ggaaaattgg tgcccgctgc ggtaacaacc
     1441 ggtcacctcc gaggagctga actagcaggg ttgtctcagc cgagaccaaa tctgatcacg
     1501 gagcgtgcag gcccgcaggg ccgacctact ctagcccagg gcgcggcggc acagccgcaa
     1561 gccctgggtt agcgtgcata tgaaccgtgg cagccccgca ggggcgaaat actgcgcccc
     1621 tgcggggctg ccatattcat cgatcgcccg ccgacccagg ccgttgacct gggctggcga
     1681 aggtcggccc gttgggccgg caatgccgcg ctagggcctt cccgcatggg tccgcggccg
     1741 acgctcggcg gcggtcgcga ccttgaacct acggctctaa cgcgccgctg gcgaacgcgc
     1801 gtataatcaa cggcggggaa tcgccgccgc aacgctccag gaacagccat gccggtgcag
     1861 atgcagttga cgcggatcat catcagcgag atcaatccgc agcaggtgat ttatcttcgc
     1921 gaggtcgagg gcgaccgcac gtttccgatc ctgatcggcc tgttcgaggc cgagagcatc
     1981 aacacgggcg tcaagcgggt gcaatcgccg cggccgcgga cgcacgagct gctcgtgcag
     2041 tcgatcgagg ccctcggcgg cgagctcgac agcgtcgtaa tcagcgagct gcgggaccac
     2101 atctacttcg ccaagctgcg ggtcaagcgc gaaggcgagc tggtcgagat cgactcccgc
     2161 cccagcgacg ccatcgccgt cgccgtcgcc tgcgacccga tcctgccgat ctacgtggcg
     2221 gaagaggtgt tggaggagag tacgggaaac taggggtcgg tgggtcggtt aatcggtgat
     2281 cggtccgtcg gttgtcaccg atttaccgac caaccgacca ccgaccgacc gaattgcaaa
     2341 ctcccccgcg ctgggctacg ctaaggccca gccacgatat atccattccg caacgggcat
     2401 ttcaggagaa tacgcacatg gctcgcaaga cttcccgccg caaatttctg ggcacttcgg
     2461 cggcgatcgg ggctggcttt tgggtcgccg gcggaatcgc gccgcgcgaa agccgggccg
     2521 cgatcgacaa gatccacttc gccagcgtcg gcgtcggcgg caagggcgcc ggcgactcgc
     2581 aggatgccgg gaaggccggc gatatggtcg ccatttgcga catcgacgac aagaacctca
     2641 atttcgccgc caaccgctgg cccaatgcca aaaagtacca cgacttccgc aagatgttcg
     2701 aggagatgga gaagagcatc gatgcggtca cggtcagcac gccggaccac tgccacgcgg
     2761 tgatcggcgc ggcggccatc agccgcggca agcactgctt catccagaag ccgctggcca
     2821 agtcgatcca cgaagtgcgg cacttgcagc ggattgcgac cgagaagaag gtcgcgaccc
     2881 agatggggaa ccagttcacc gccgacagca acctgcggcg agcggcccag gtcatcaagg
     2941 acggacacgt cggcaccgtg aaagaggtgc acgtctggac caaccgaccg gtctggccgc
     3001 agg
//
LOCUS       DASRJB010000227         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_348839, whole genome shotgun sequence.
ACCESSION   DASRJB010000227 DASRJB010000000
VERSION     DASRJB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348839"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFB80_06305"
     CDS             <1..370
                     /locus_tag="VFB80_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33411.1"
                     /translation="TFVGGYWHPRWAGESRWTIRGSQRWVHWDANRKGTNGALEIHGP
                     QPQWHAMEDVFTTAEDKSPGYGGRRGFLLVQDWLDCIRTGRRDNRATCRGTLATLELI
                     DAIFQSSREGKRIECRIEPA"
     gene            628..2046
                     /locus_tag="VFB80_06310"
     CDS             628..2046
                     /locus_tag="VFB80_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33412.1"
                     /translation="MTRPHSALLSLLLSVTCAAAADWPQFRGPRGGQAVVDGPLPTEI
                     APDKLVVWKTELPPGHSSPAIVGGRIFLTAVREKEHLETLCLDRASGKILWRVESPHK
                     TLEQIHGIGSYAQPSPAADAERVVVLFGSSGLYCYDHSGKELWKQPMGPFNDEFGAAA
                     SPILDGERVILCQDHDSGSFLAAYDKRTGQELWRTDRSEFSRGYSTPVVWQVEGKKQI
                     VVAGTLRVAGYDYQSGKEMWTVRGLSRVNCMTVTVGDDNRLFAAGWSAGAEPGDRVRL
                     EPFADYLSVNDENKNGTLETSEVSNSLALKTRFVQCDRNKDGKITQPEYDEFQNLFDK
                     SENVVLAIRPGGRGDISHTHVAWKFEKFVPFCASPLAYRGRVFTIKDSGILSCLDAAT
                     GKAHKTGRVTGTANYYASPVGGDGKVYLLSQRGTLSVVSATDQWQVIHSAEFGEEVYA
                     TPALLDGRIYLRTAGHLYCFGR"
     gene            complement(2069..3520)
                     /locus_tag="VFB80_06315"
     CDS             complement(2069..3520)
                     /locus_tag="VFB80_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33413.1"
                     /translation="MSTPAYDPDYPYTPQPAVLEGPPGPRKPLPWAVVWRWTKRLAIA
                     AVTCVLLVAVSVGYVASRAWRQADSIARLKQLGCLIDYAHEEDLLEESALARDLREAF
                     GDEVWGTVVGVRADQSFLFRGRLTADETAAICKTCRQFGSLRSFAIASDSFRIEQIAD
                     WPQLPLLQKLSIHSQALSDDDVARIGKLSQLRELELTAPRLTAASLSHLAGLTHLDSL
                     TLRDARLGGAAPRVGQGFPELEYLTIKNATELSDEAFVSLGPLAGLNHAVLDGTTIGD
                     RAVKHLANCQNLNLLSLVGTRVTDASLTEFRKLADRALSQLDLEGTAVTDRGLAALAG
                     SELMSLSLDRTAVTDEGVREIAGMRGLDFLSLRDSRVTGAGVAHLRFSSLSVYLDGAP
                     LTPEGIRALAQAKADVITVARTTFGDADLMLFANNDVLKSLDVTGAKVTEEGVRSFRT
                     ARAKRFATAGREDQLLLFSDFAEEPPADSGPGF"
     gene            complement(3517..>3761)
                     /gene="mtnA"
                     /locus_tag="VFB80_06320"
     CDS             complement(3517..>3761)
                     /gene="mtnA"
                     /locus_tag="VFB80_06320"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910912.1"
                     /note="isomerizes methylthioribose-1-phosphate into
                     methylthioribulose-1-phosphate; involved in methionine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZN33414.1"
                     /translation="TTFDLSLETGDQIPIEERSPEEITHGFGRQTAPDAIGVYNPAFD
                     VTPARLIKAIITERGVIEPVSRETIAAMLKSPAAAV"
BASE COUNT          694 a         1203 c         1211 g          653 t
ORIGIN      
        1 gacgttcgtt ggcggctact ggcatccgcg gtgggccggc gagagccgct ggaccatccg
       61 cggcagccag cggtgggtcc actgggacgc caatcgcaaa ggcaccaacg gagcgctcga
      121 aatccacggc ccgcagccgc agtggcacgc gatggaagac gtattcacca cggccgagga
      181 caaatcgccg ggctacggcg gccgccgcgg ctttttgctg gtgcaggact ggctcgactg
      241 catccgcacc ggtcgccgcg acaatcgcgc cacctgccgc ggcacgctgg ccacgctgga
      301 gctgatcgat gcgatcttcc agagcagccg cgagggcaag cggatcgaat gccggatcga
      361 gccggcgtag ggttattgag ccctgcatag ccaatgtcgt ctaacccgga gccggagccg
      421 acggcgcccg cgggaatcgc ctgcgcctcc gcctacgggc taaacgagga atcgccaatc
      481 ggaatgcaag ggccaataaa cacgaagggc accaaggaca cgaagaagca agccgagtgg
      541 agtgagtggg atcggcagat taagatttta cttccctccc acctttgtgc tcttggtgtc
      601 cttcgtgttt aaccttccgc cccacgcatg acccgccctc actccgccct tctgtctctc
      661 cttctctccg tcacttgcgc agcagccgcc gattggccgc agttccgcgg gccgcggggc
      721 gggcaggccg tcgtcgatgg tccgctgccg acggagattg cgccggacaa gctggtggtc
      781 tggaagaccg agctgccgcc ggggcactcc tcgccggcga tcgtcggcgg gcggattttc
      841 ctgaccgctg tccgcgagaa agagcacttg gagacgctct gcctcgaccg ggccagcggc
      901 aagatcctgt ggcgcgtgga atcgccgcac aagacgctgg agcagattca cggcatcggc
      961 agctacgccc agcccagccc cgcggccgac gcggagcggg ttgtcgtgct gtttggaagc
     1021 agcggcctct attgctacga ccacagcggc aaagagctct ggaaacagcc gatgggtccg
     1081 ttcaacgacg aattcggggc cgcggcctcg ccgatcttgg acggcgagcg ggtgatcctc
     1141 tgccaggacc acgacagcgg ctcgttcctg gcggcctacg acaagcggac cggccaggag
     1201 ttgtggcgga cggaccgctc ggagttctcc cgcggctatt cgacgcccgt cgtgtggcag
     1261 gtcgagggca aaaagcagat cgtcgtcgcc ggcacgctgc gtgtcgccgg ctacgactat
     1321 cagagcggca aggaaatgtg gaccgttcgc ggcctgtcgc gcgtgaactg catgacggtc
     1381 acggttggcg acgacaaccg gctcttcgcc gccggctggt ccgcgggagc cgagcccggc
     1441 gaccgcgtgc ggctggagcc gttcgcggac tatctgtcgg tgaacgacga gaacaagaac
     1501 ggaacgctgg agaccagtga agtttcgaac tcgctggccc tcaagacccg cttcgtgcag
     1561 tgcgaccgca acaaggacgg caagatcacg cagccggagt acgacgagtt ccagaacttg
     1621 ttcgacaaat cggagaacgt ggtgctggcg atccggcccg gcggccgcgg cgacatcagc
     1681 cacacgcacg tggcctggaa gttcgaaaaa ttcgtgccgt tctgcgcctc gccgctggct
     1741 taccgcgggc gggtattcac gatcaaggac agcggcatcc tgagctgcct cgatgccgcg
     1801 acgggcaagg cccacaaaac aggccgcgtc accggcaccg cgaactacta cgcctccccc
     1861 gtcggcggcg acggcaaggt ctatctgctc agccagcgcg gcacgctctc ggtcgtctcc
     1921 gcgaccgacc agtggcaggt gatccactcg gccgaattcg gcgaagaggt ctatgccact
     1981 cccgctctgc tcgacggccg catctacctg cggaccgccg ggcacctgta ctgcttcggc
     2041 cggtagcgcg gcagcttcca gctgccgatc agaagcccgg acctgagtct gccggcggtt
     2101 cttcagcaaa gtccgagaat agcagcaatt gatcctcgcg acccgcggtt gcgaatcgct
     2161 tggcccgcgc ggtgcgaaag ctgcgcaccc cctcttccgt aactttcgcg ccggtgacgt
     2221 ccagtgactt gagaacgtca ttgttcgcaa agagcatcag atccgcatcg ccgaacgtag
     2281 tgcgtgccac cgtgattaca tcggccttag cttgggccag tgccctaatt ccttccggag
     2341 ttagcggagc gccatcgagg tagaccgata gcgacgagaa acgcaggtgc gccacgcccg
     2401 cgccggtgac ccgactatcg cggagtgata ggaaatcaag cccgcgcatg cctgcgattt
     2461 cccgcactcc ttcgtcggtc acagccgtcc tgtccagcga aagactcata agctcagaac
     2521 ctgcgagagc agctagtccg cgatcggtaa ccgcggtgcc ttccaaatcc aattgggaca
     2581 gcgcccggtc ggccagtttt ctgaactccg taagagacgc atccgtgact cgcgtcccaa
     2641 ccagcgacag caggttgagg ttttggcagt tcgccagatg cttcaccgcg cgatcgccga
     2701 tggtagtccc gtcgagcacg gcatggttca acccagccaa tggtcctagg ctgacaaagg
     2761 cctcgtcgga tagctcggtt gcgttcttga ttgtcagata ctccaactca ggaaaacctt
     2821 gtccaactcg cggagctgcg cctcccagcc tcgcgtcgcg cagtgtgagc gagtcgaggt
     2881 gcgtgagccc ggccagatga ctcaggcttg ctgcggtcag tctcggcgct gtcagctcca
     2941 gctcgcgcag ttgggacagt tttccaattc gcgcgacatc gtcgtcagaa agcgcctggc
     3001 tgtggatgga cagcttttgc aggagtggca gctgcggcca atccgcaatc tgctcgatcc
     3061 gaaacgaatc gctggcgatc gcaaatgatc gcagggaccc gaactgacgg caggttttgc
     3121 agatggcggc tgtttcgtcg gcggttagcc gtccgcgaaa caggaatgat tgatcggccc
     3181 gtacgccgac gacggtcccc cagacttcgt cgccaaacgc ttcgcgcaag tccctcgcaa
     3241 gggcactttc ttccagcaaa tcctcctcat gggcgtagtc aatgaggcag ccgagctgct
     3301 tcagccgagc gatcgagtcc gcctgccgcc aagcgcggct ggcgacgtag ccgacggata
     3361 cggcaaccag cagcacgcac gtcaccgccg cgatcgccag ccgcttggtc caccgccaga
     3421 cgaccgccca gggcagcggc tttcgtgggc cgggtggtcc ctctaaaacc gccggctgcg
     3481 gcgtgtaggg atagtcggga tcgtaggcgg gggtgctcat actgcggcgg cgggactctt
     3541 tagcatcgcg gcgatcgttt cgcggctgac gggttcgatc acgccgcgct cggtgatgat
     3601 ggctttgatc agccgggcgg gggtgacgtc gaaggccgga ttatagacgc cgatagcgtc
     3661 gggggcggtc tggcggccga agccgtgggt gatttcctcg ggggagcgtt cctcgatggg
     3721 aatctggtcg ccggtttcga gcgaaaggtc gaacgtggtg c
//
LOCUS       DASRJB010000228         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1758752, whole genome shotgun sequence.
ACCESSION   DASRJB010000228 DASRJB010000000
VERSION     DASRJB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758752"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFB80_06325"
     CDS             complement(<1..396)
                     /locus_tag="VFB80_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33415.1"
                     /translation="MPSSASGIRIWRAFDAHHDRGAVFATTACGEMVYGEKRQQACAH
                     NAHNRRRLDFVATLFSKRSCVVFCARKFFSAVCAHYPARLLAAVLLLLCATAWADDFP
                     QPANQGAGAGERPLSAEQAAAGFRLPPGFK"
     gene            complement(451..>2338)
                     /locus_tag="VFB80_06330"
     CDS             complement(451..>2338)
                     /locus_tag="VFB80_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZN33416.1"
                     /translation="ADVPSKEPSPVPQANLDQKNPQPPSTPPEVAPPGPIESIPLVLP
                     PAVPGPAVEIEGKARVENSVAPVTGNTGKASKKKGAKAAADRQWLTPARKAAPTAAQL
                     AKAHEQIAALERPPPKTDQERGTLAANLLAKGKDQQLPDADRYAALEKACELAASAGD
                     AKLLAQAVGALTQAFELDPRPLEGNWLVQAAENVGTAPQVDALLVALQPAIAFAVSEG
                     DFEEALRLADAALAVCQKPQGQQHREWAIWGRQDIARQHAARKTMNDALATLQPHDPA
                     AHLAAARWLILERNDWPRALPHLAQASDPQLKQAAALDLANPATSEAQLAAADAWHAA
                     GKGPPGEAFCLARALHWYELARRGSLPKPESVKAERRIAELRSSRAVAALQLRLKSAV
                     GIGSLSDRLSPAVRRHCVAAFTFEASDLFSANGETTLIDRSGRRHHGRVYGTSPSFGK
                     VGASYLFDGPEDVVECSDAPSLNPTSGLTLCAWVRPQQVSQGTVDYIISKDDWKVGTP
                     RGFVLRLSYRGRPNMTLGSGGWWGPTVREAIKLNEWTHLAGVYDGRQIVVLVNGEEVG
                     QGACAGQPTPSPFDLRIGKGTYDKNRRFQGEIDEVAIFDMALTAADIKTIYELGTKGQ
                     SLAN"
BASE COUNT          361 a          806 c          769 g          402 t
ORIGIN      
        1 cttgaagccc ggcggcaggc ggaaccccgc ggctgcctgc tcggccgaaa gcggccgctc
       61 gccggcgccc gccccctggt tcgccggctg cggaaaatcg tcggcccagg ccgtcgcgca
      121 cagcagcagg aggacggccg ccagcagacg ggcaggataa tgcgcgcata cggcggaaaa
      181 aaacttccga gcgcaaaaaa cgacgcaact tcttttggag aaaagagttg cgacgaaatc
      241 tagccgccga cgattatgcg cattatgcgc gcacgcctgc tgacgtttct cgccataaac
      301 catttccccg caggcagttg tggcgaaaac cgcgccgcgg tcgtgatgcg catcaaaggc
      361 acgccaaatt cgaatgccgc tggcggagga aggcatgggg aacattctat gttaattcta
      421 tacaattgtc aattgttcag aacccatccc tcagttcgcc agcgactggc ctttggtgcc
      481 cagctcgtaa atggtcttga tatcggcggc ggtcaatgcc atgtcgaaga tcgcgacctc
      541 gtcgatctcg ccctggaagc ggcggttctt gtcgtaggtg cccttgccga tccgcaggtc
      601 aaaggggctg ggcgtgggct gccccgcgca agcgccctga ccgacttcct cgccgttgac
      661 gagcaccacg atctgccgcc cgtcgtagac gcccgcgaga tgggtccact cgttcaactt
      721 gatcgcttcg cggacggtcg ggccccacca gccgcctgac cccagcgtca tgttcggccg
      781 gccgcggtac gagagccgca gcacgaagcc gcggggcgtg ccgaccttcc aatcgtcctt
      841 gctgatgatg taatcgacgg tgccctggct cacctgctgc ggccggaccc aggcacacag
      901 cgtcagcccg gacgtgggat tcaggctggg cgcgtcggag cactccacca cgtcctccgg
      961 cccgtcgaac aggtagctcg ccccgacctt gccgaaagac gggctcgtgc catagacgcg
     1021 gccgtgatgc cgccggccgc tgcgatcgat cagcgtggtt tcgccgttag cggagaacaa
     1081 gtcgctggct tcgaaggtaa aggccgccac gcaatgccgc cgcacggccg gcgaaagacg
     1141 atcgctgaga ctgccgatgc cgaccgcgga cttcagcctg agctgcaggg cagctaccgc
     1201 tcgggaactc cgcagctcgg cgattcgccg ctcggccttg acgctctccg gctttggcag
     1261 gctgccgcgg cgagcgagct cgtaccagtg caaggcccgc gccagacaaa acgcctcccc
     1321 gggcggtccc ttgcccgcgg catgccaggc gtcggccgcc gcgagctggg cctcgctcgt
     1381 ggcgggattg gcgaggtcga gcgccgccgc ctgcttgagc tgcggatcgg atgcctgcgc
     1441 cagatgcggc agcgcccgcg gccagtcgtt ccgctccagg atcagccacc ttgctgctgc
     1501 caggtgcgcg gccggatcgt ggggctgcag ggttgccagc gcgtcgttca tcgtctttcg
     1561 cgccgcgtgc tggcgggcga tatcctgcct gccccagatg gcccactcgc ggtgctgctg
     1621 tccttgcggc ttctgacaca ccgccagcgc ggcgtcggcc agacgcaagg cctcttcgaa
     1681 gtccccctcg gagacggcga acgcgatcgc cggctgcagg gccacgagca gcgcatcgac
     1741 ctgcggcgcg gtaccgacat tctccgcggc ctgcacgagc cagtttcctt ccagcggccg
     1801 cggatcgagc tcgaaggcct gcgtcagcgc gcccacggcc tgcgcgagca gcttcgcatc
     1861 tccggccgag gcggccagct cgcaggcctt ctccaacgcg gcgtagcgat ccgcgtcggg
     1921 caactgctgg tccttgcctt tcgcgagcag gttggcggcc agggtcccac gctcctggtc
     1981 cgtcttgggc gggggccgct cgagcgccgc gatctgctcg tgggctttcg cgagctgcgc
     2041 agcggtcggc gccgctttcc gggccggagt cagccactgc ctatcggccg cggccttcgc
     2101 accctttttc ttgctggctt tgccggtatt tcccgtcacc ggggcgacac tattctcaac
     2161 tcgggccttg ccctcgatct cgaccgcagg accgggcacg gctggcggca gcacaagcgg
     2221 tattgactca atcggcccgg gaggcgcgac ctcggggggc gtgctgggcg gctgcgggtt
     2281 cttctggtcg aggttggctt gcggaaccgg cgacggctct ttcgacggga catcggcg
//
LOCUS       DASRJB010000229         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2604253, whole genome shotgun sequence.
ACCESSION   DASRJB010000229 DASRJB010000000
VERSION     DASRJB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604253"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFB80_06335"
     CDS             complement(<1..296)
                     /locus_tag="VFB80_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZN33417.1"
                     /translation="MAAEKWDETGRVWEEDHGLLDEEQVAVCRRADVAEPPSPVPTRM
                     ISNGEYMPAPQTRQQKQVESRVGELAEAASRKLGISRRQFLAGSGGMAAALLAM"
     gene            805..1518
                     /locus_tag="VFB80_06340"
     CDS             805..1518
                     /locus_tag="VFB80_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33418.1"
                     /translation="MSVSARNSALAAGCIFLTLAGGWSAACAQENKPAGQKPAQLPPG
                     RIRGPSEQVAEIIISGRLLRNEGKYDEALAQFDKALKTARFTMDRSGEAWAISNVATV
                     YRYRAEKEADKAAELIKKSAEHYEQAANIARENADKHNEAYATLYLGVLAAMRKDPDQ
                     ALRRYAVAMPLFEAEGDDYYIGRTYAYQARAMLLRRDPLKALELFEKALPLLREVGML
                     NEAAEVVGEMQAIYAAIVR"
     gene            complement(1524..>3451)
                     /locus_tag="VFB80_06345"
     CDS             complement(1524..>3451)
                     /locus_tag="VFB80_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33419.1"
                     /translation="LLEQHRPAPGAEDLRCFEWFHLWHLCQHAQRGVLRGDGRPVRAI
                     AAAPDGSVWASAGDGGTIHLWDPATLQSLGTLRLDAAKVHSLAISSDGQRLAAACGER
                     PAELWDLATRQRLASLGKATEVLFSPDGGTLYSSGGRRLVELWNGRTGARLGLLSGNA
                     GLPNCLAVSGDGSSLALAGNDRRVCLADLTSTEKAFVEVGTHRSYVLCLAFSPDGAAL
                     ISGSEDGYVQLWDVAGRKAQLEQPLRQHTGAVNAAAWSPDGRHVATGSGDGSIKVWNA
                     QTREVVLQQGHPGPVYCIAFTPDGNSLLTGGEDGGVRVWNLKSRPEPLVLSGHERWVP
                     ALSISGDGRWLASSSSDGTARLWRLAGPVADDNAPLQPLVLKPDSQPVPAWMSREGMA
                     LGGEPDRVMGVGFTSSSQQVITADFGGRVRLWDAATSNEAGEFEPADGPIWALAISPD
                     GQTLAAAGYTANVLTVWDIASRKKRTVLRGHTDRVWTVAFSPDGRRIASAANDKTVRM
                     WDAFSGSPQGVVHLPVEFLFALAWSSDGRQIAASGDDCRIRLIDAAAAKELKPIGQHP
                     AAIRGLAFFPDGKTLAAGGDDGAVRLWDLATRQERATLRVRGSSIWSLAVAPDGRQVA
                     AGDGDGEITVWNRADRE"
BASE COUNT          621 a         1161 c         1138 g          531 t
ORIGIN      
        1 ttcatcgcca gcagggccgc cgccattccg cccgatccgg ccagaaactg ccgccggctg
       61 attcccagct tccggctggc cgcctcggcc aattccccca ctcgcgactc gacctgcttc
      121 tgctgccgcg tctgcggcgc cggcatgtac tcgccgttgc tgatcatgcg cgtcggcacc
      181 ggcgaaggcg gctccgccac atccgcccgc cgacaaaccg ccacctgctc ctcatcgagc
      241 agcccatgat cctcctccca aaccctcccg gtctcatccc atttttcagc agccatgatc
      301 gcctccgcgc aagaagccaa ccgccgccaa actggcgcta tctctaacgc gctgccgtgc
      361 tgcccgcaag ctgcccgtga gcctttcgct gccgcgctga acagacccta accgggtggt
      421 tgcgggcggc ttccttgaac gagcactttg cggcaagcca tagtcgcaag tcttgtttgc
      481 caaaggagtt gcgtctagca catttgtgaa gaacgtggat tagcaacaaa agtgacataa
      541 gggttagaga ccgtcgttga acaggtgcga ttggaatgtt ttggagtgac ccatcggagg
      601 cctctcgtcg tcaaggtgca aacggcgcgg cgttgcgggc gactcccggt cccgcggctt
      661 gccaaaaagg cagcctgccg cgcaaacgga actatactgc aaatagcgta ccaatgtaca
      721 caattgcgta cgggcccaca aaaatggaga cttgtcggca ccgccgtccc gccggggctg
      781 tgcaggtcta tcaaggagcc gccgatgagc gtgagcgccc gaaattcagc gttagccgct
      841 ggttgcattt tcctgacgtt ggccggcggc tggtcagccg catgtgctca ggagaacaaa
      901 ccggcgggac agaagccggc ccagttgccg ccggggcgga ttcgggggcc ttcggaacag
      961 gtcgcggaga tcatcatcag cggccggctg ctccgcaacg agggcaagta cgacgaggcc
     1021 ctggcccagt tcgataaggc gctcaagacg gcccgcttca cgatggaccg ctccggcgaa
     1081 gcctgggcaa tcagcaacgt cgccaccgtg tatcggtatc gcgccgaaaa ggaggcggac
     1141 aaggcggccg agctgatcaa gaaatcggcc gagcactacg agcaggcggc aaacatcgcc
     1201 cgcgaaaatg ccgacaagca caacgaggct tacgcgacgc tctacctcgg cgtactggcg
     1261 gccatgcgga aagatcccga ccaggcgctg cggcggtacg ccgtcgcaat gccgctgttc
     1321 gaggcggaag gcgacgacta ttacatcggg cggacgtatg cctaccaggc ccgcgccatg
     1381 ctccttcgcc gcgatccact caaggcgctg gaattgttcg aaaaggcgct gccgctgttg
     1441 cgtgaagtcg gcatgctcaa tgaagcggcc gaggtcgtgg gcgagatgca ggccatctac
     1501 gcggcgatcg tgcgttaggg acatcattcc cggtcggcgc gattccagac ggtgatctcg
     1561 ccatccccgt cgcccgcggc cacctgccgt ccgtctgggg cgacggccag cgaccagatg
     1621 gaagagcctc ggacgcggag cgtcgcccgc tcctggcgcg tggccaggtc ccagagccgc
     1681 acggcgccgt cgtcgccgcc ggcggccaaa gtcttgccgt cggggaaaaa ggccagcccg
     1741 cggatggccg ccgggtgctg gccgatcggc ttgagttcct tggcagccgc cgcgtcgatc
     1801 aagcggatgc ggcaatcgtc gccgctggcg gcgatttggc ggccgtcgga ggaccaggcc
     1861 agggcgaata gaaactcgac gggcaggtgg acgacgccct gcggcgatcc gctgaaggcg
     1921 tcccacatgc ggaccgtttt gtcgttggcc gcagaggcga tgcggcgtcc gtccggtgaa
     1981 aaggccacgg tccagacgcg gtcggtatgt ccgcggagga ccgttcgctt ctttcgcgac
     2041 gcaatatccc agacggtgag cacgttggcg gtataaccgg cggcggccag cgtctggccg
     2101 tccggggaaa tcgccagcgc ccagatcggc ccatcggccg gctcgaactc gcccgcttcg
     2161 ttgctggtgg cggcatccca cagccgcacc cgcccgccga agtcggcggt aatcacctgc
     2221 tgcgaggagg aagtgaagcc tacgcccatg acgcgatcgg gctcgccccc cagcgccatc
     2281 ccctcgcgcg acatccaggc aggcactggc tgcgaatcgg gcttgagcac gagcggctgc
     2341 aaaggagcat tgtcatccgc gactgggccg gcgagccgcc agagccgggc cgtaccgtcg
     2401 gacgatgagg aggccagcca ccggccgtcg ccagagatgg aaagcgcggg aacccaccgc
     2461 tcgtgcccag acagcaccag cggctcggga cgcgacttca gattccagac gcgcacgccg
     2521 ccgtcttcgc cgcccgtgag cagcgagttg ccgtcgggcg tgaaggcgat gcaatagaca
     2581 ggaccgggat gaccttgctg aaggacgact tcgcgcgtct gggcattcca gactttgatc
     2641 gagccgtcgc cgctgcccgt agcgacgtgg cgtccgtcgg gcgaccaggc ggcggcgttg
     2701 acggcgccgg tgtgctgccg cagcggctgc tcgagctggg ccttgcggcc cgcgacgtcc
     2761 cagagctgca cgtagccgtc ctcgctgccg gagatgagtg ccgctccgtc cggcgagaac
     2821 gccaggcaca gcacatagga gcggtgcgtg ccgacttcca cgaatgcctt ctcggtcgaa
     2881 gtcagatccg cgagacatac gcggcggtcg ttgcctgcca gggcaaggct gctgccgtcg
     2941 ccggaaacag ccaggcaatt gggcaagccg gcgtttcccg agagcaagcc gagccgggct
     3001 cccgtccgtc cattccagag ctcaacgagc cgccggccgc cgctggaata gagtgtgccg
     3061 ccgtccggcg agaacaggac ttccgtggcc ttgccgagac tcgccaggcg ttgccgagtc
     3121 gccaggtccc acagctcggc aggacgttcc ccgcaggcag ccgccagccg ctggccgtcg
     3181 ctcgagatcg ccagcgagtg gaccttggcc gcatcgaggc ggagcgtgcc gagcgattgc
     3241 aaggtcgccg gatcccagag gtgaatcgtg ccgccgtcgc cggccgaggc ccagacgctg
     3301 ccgtccggtg cagcggcgat ggcccgcacg ggccggccgt cgccgcggag cactccccgc
     3361 tgggcatgct gacagagatg ccagagatga aaccactcga agcagcgcag gtcttccgca
     3421 cccggagctg ggcgatgctg ctccaagagc g
//
LOCUS       DASRJB010000230        11043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1759192, whole genome shotgun sequence.
ACCESSION   DASRJB010000230 DASRJB010000000
VERSION     DASRJB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759192"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VFB80_06350"
     CDS             <1..1317
                     /locus_tag="VFB80_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33420.1"
                     /translation="YAVAAYGSPEEQDLPIFVDLDVLLDMEDHAQSDTSVELGGVLLG
                     GHYEDEDGKPFVIVTDSLRAQHYESTKGSFKFTHDTWSQITRERDEFPVELQMVGWYH
                     THPDWGVFLSGMDMFICDNFFNKKLDVAYVIDPCRGDRGMFQWTGDPRHRVRRTGGFF
                     VTASRFRAADLEYTVGELCGAMPVTTTSRGSAAAGHAPVIHLHQPQSPQPPWQGIAIA
                     GALALQLALVGLIAWKTLVPPPADAKHEDKIVAAIEKFAGDSFAAQQAAQSAKERSRG
                     QAEILDQVVSALRSGESGAVSKALSQHDQAQRLSADVEARDGQIHSLQVKMEELRRSF
                     QTQKAKADADLLKQGEEIANLTKERDKWVTKYDDVAAKLEKYLPPSKTGTTAGKASPY
                     TVWYWVGGAVLAALALVCGVFWIGGQMSAEKTEESEELRPEEPKNQ"
     gene            1412..2104
                     /locus_tag="VFB80_06355"
     CDS             1412..2104
                     /locus_tag="VFB80_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HZN33421.1"
                     /translation="MDASSRIARLNADRELLAALDAASSIFSLEVTGDPPDRYTLTFR
                     GKGLGRDAAASSDVTVIELHQIDLRMPYAYPASPPDIRWITPLWHPNVSFSGFVNPAD
                     IGLVWTSEMPLDLVCERLWDVARGQFLNLNKATNYAAKNWFEKECTYQLPLDPRSLRD
                     GGGARSSSNVVRYERRAGQGVQFAGAAAIGDVMFIDENTPTPQMPARQPYIPSARRRP
                     RSGDDVMYIGPE"
     gene            2245..3120
                     /locus_tag="VFB80_06360"
     CDS             2245..3120
                     /locus_tag="VFB80_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HZN33422.1"
                     /translation="MECFVIAVILFGSVIAMAIATLASARGGGQWNEAFKHVARRFHG
                     TLHPGGWFSQPAVWMQHGESHARLTVTTLRGSGGERLLQMTIQQRDLRTQCEIYYHQS
                     RPALVPPTRGLFPVEFDWEEFRRRWIVKAADGDEVRHLLTDGVRLAIEQLWRHPLPGE
                     TTISLSPSWLVVRKLWNSPRGIDIEEFAERTLMLSDQLHLAAASGIEFVVSDQPQLLD
                     EARCGVCGDSLAQSVVVCRRCNTPHHRDCWDYGGGCATYGCGGRECFIPGVAPLAAPH
                     WDAATQPTERPAKPR"
     gene            complement(3122..4735)
                     /locus_tag="VFB80_06365"
     CDS             complement(3122..4735)
                     /locus_tag="VFB80_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33423.1"
                     /translation="MAKNFRKPATAAKKVKSVNGHGLAHDDGFADQDEAGPGFATAEE
                     ENEPGLDDEEEEEDLHGDDEASVDATDDPVRMYLMQMGQIPLLNRAQEIASAQVIERT
                     RLRYRHSMLATDYVLQGAVEFLERVRDGQLRLDRTIEVSVTNTTEKKRIMKRIWPNVA
                     TLRHLLNQNHRDFRIAVSKQFPMKQRRAAWARLTRRRNKAVRLVEEMNLRNNRLQPLF
                     GKLAEILLRMKTLKMQIREARVVGLSGGRTEPELRKELYYLMRISLETPATLRRRIER
                     TEIYREEYDAAKRDLSAGNLRLVVSIAKKYRNRGLSFLDLIQEGNTGLMRAVDKFEYA
                     RGYKFSTYATWWIRQAITRAIADQSRTIRVPVHMIDTMSRVRSVNRTLLQELGREPTA
                     EETARRAGLSLEDARCILKMSRQPLSLDQPVGDHEDSYFGEFLADHRDDDPLYDTHQQ
                     ALKNRIDEAMQGLNYREREILRLRYGLTDGYSYTLEEVGKIFSVTRERVRQIETKAVR
                     KLQQPYRCRSLASFIDGLEAPTDATPADA"
     gene            5038..7614
                     /gene="mprF"
                     /locus_tag="VFB80_06370"
     CDS             5038..7614
                     /gene="mprF"
                     /locus_tag="VFB80_06370"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HZN33424.1"
                     /translation="MTRSDEGNGSWLARLRPWLGPLVAVVVFAAAIVLLRQELKHHSW
                     RQIMREVGAVPPQRIALAIGLTALNYVVLSGYDALAVRYVKHPLRPTQILLGSFVGYA
                     MSHNLGWMLGGTTSRYRLYSTWGFSAVEIIKLFAILGLTFWSGYCFLAGVVFLLDPLP
                     MPPQLHLPLASTFWLGPILLGMLAVYLVACAIGRPIVIRGWRIELPALTLALGQIAVA
                     SCDLLLASAVMYVLLPAGMAISYWRFANVVLLALASAIISHVPGGVGVLELVVVKLVA
                     PDDPAALVGGLLAFRAIYYLLPLMTALGLLGGHELIVHREQAHGLVTRLGQFFPHIAP
                     RLLAFACFIAGVVLLLSGVTPGEDSRLDWLERFLPLGVLEASHFLGSVVGVVLLLLAR
                     GLQRRLDSAYWLAIVLLAAGIAASLLKGFDFEEAVYLALMLAALLPCRRHFYRKSSLL
                     SERFSPQWTVAVLLVLVCALGLALFAFERVEYSNELWWKFAFTASAPRTLRALAGVGI
                     AVFSIALARLLRAKPKPPAAPTPAELVEATQLARRSPRTYAQLVGLGDKSLLFSEDRQ
                     GFLMFAEEGRSWVVLGDPVGPDEVAEELAWEFRELCDAGGRWPVFYQVETERLPVYIE
                     LGLSILKLGEEARVPLAGFSLEGRERKSLRHTCTRLEREGCTLEVVEPPLAEPLLNEL
                     AAISDDWLRRKNTREKGFSLGFFERGYVRQSAVALVRQSDRVVAFANVLRGGDKEELS
                     VDLMRFADGAPGGAIDYLFAQLMLRGHEESYRWFNLGVAPLAGVEGHSLAPAWNRFAE
                     IVYRHGEHFYNFQGLRDYKDKFDPVWTPKFLACPGGLRLPMILTNLATLISGGPKGLV
                     GK"
     gene            7745..8371
                     /locus_tag="VFB80_06375"
     CDS             7745..8371
                     /locus_tag="VFB80_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZN33425.1"
                     /translation="MVALTWFFIFCAVVGGTVLICQFVLTVLGLSGDHGLDAGHDLTG
                     DAGHDVHAVGAHGAEGDGHDQAGHHGSSWLFAMISFRTLVAAAAFFGLVGLTAQSAGQ
                     PVGVQLLLAALAGFGAMYGVHWLVRTMGRLGEDGTQRVQGAVGQEGTVYVPIPPGKSQ
                     AGKIQLKLQNRLVEYEAVTNNPERLATGTRVRVVAVAGNQLEVEPLKV"
     gene            8462..10093
                     /locus_tag="VFB80_06380"
     CDS             8462..10093
                     /locus_tag="VFB80_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN33426.1"
                     /translation="MTSSILFAQMPAEVMGMLIATTFALLGILVVGLLIVFVRGFKRC
                     PSNRVLVIYGRTGKAGATKCVHGGADFVWPLIQDYAYLSLEPIQIEVPLRGALSTENI
                     RVNVPSVFTVAIDTKPEVMQNAAVRLLGLSVPEIRKQAEEIIFGQLRQVIASMGIEEI
                     NRDRDKFLQHIQHSLEPELAKIGLQLINVNITDITDESGYIDAIGQKAASLAIQQARG
                     DVADNEKMGETRVASAERDKSVQVANARKEQSIGTREAQREQAVRIATLEKEQSIGEK
                     TAAFEKDSQVKQAEQQMRVQVAEADAGAIDGENQAAARVAASQAELAIKKAEAYERGE
                     GRRKQAEAHVMEIQNHALAKAALAEAERVEAEQRAKLEAPAKAQKARVVVDAEAAADQ
                     RRIMAKAEADAIYLKLEAEARGQYEILAKKGDGLKQIVEACGGSTQAFQMLMLEHLDN
                     LADCSARAISNIKFDKVVVWENGGSNGRGSTADFLSSMAKTMPPMMQVMRDIGGIELP
                     ESLVKLGGDATNSGVAVATNGNAPPAAAGKDKGKA"
     gene            10494..>11043
                     /locus_tag="VFB80_06385"
     CDS             10494..>11043
                     /locus_tag="VFB80_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33427.1"
                     /translation="MPSQRRLGISASCLANWLAVLPVLLPLPVAAADLAELTQLFRAG
                     KYAECVTGAAAEIENARYSENLRLLKLRAEMELGRYGDALKTLDAALKDFPTSIHLRW
                     VGRSVCRLNQQPERSAAFETQIAALLKQSPWQYSDAVNQVVAGRFYLSQGVDPKVVLN
                     SSYVEVRKRQPTYVDVYLAIGDL"
BASE COUNT         1825 a         3570 c         3682 g         1966 t
ORIGIN      
        1 tatgcggtcg cggcttacgg ctcgccggag gagcaggacc tgccgatctt tgtcgatctc
       61 gacgtgctcc tggacatgga ggaccacgcc cagtcggata cgtcggtcga attgggcggc
      121 gtgctgctcg gcgggcatta cgaggacgaa gacggcaagc cgtttgtcat cgtcaccgac
      181 agcctgcggg cccagcacta cgagagcacc aagggctcgt tcaagttcac gcacgacacc
      241 tggtcgcaga tcacccgcga gcgagacgaa tttccggtcg agctgcagat ggtcggctgg
      301 tatcacacgc accccgattg gggcgtgttc ctctccggca tggacatgtt catctgcgac
      361 aacttcttca acaagaagct ggatgtcgcg tatgtgatcg acccgtgccg cggcgaccgg
      421 gggatgtttc agtggacagg tgatccccgc catcgggtgc ggcgaaccgg cggatttttt
      481 gtcacggcca gccgatttcg ggcggcggat ctcgaataca ccgtagggga gctgtgcgga
      541 gccatgcccg tcactaccac cagccgcggc tcggcagccg ccggccacgc acccgtcatt
      601 cacttgcatc aacctcagtc gccgcagccg ccgtggcagg gaatcgcgat tgccggggcg
      661 ctcgcgctgc agctggccct ggtcgggcta attgcctgga aaacccttgt cccgccgcca
      721 gccgacgcca agcacgaaga caagatcgtc gcggcgatcg agaagttcgc cggcgactcg
      781 ttcgcggccc agcaggccgc ccagtccgca aaagagcgct cccgcggcca ggccgagatt
      841 ctcgaccagg tcgtcagcgc cttgcggtcc ggcgagagcg gcgcggtcag caaggccctg
      901 tctcagcacg accaggccca gcggctctcc gccgacgtcg aagcccgcga cggtcagatc
      961 cactcgctcc aggtcaagat ggaagagctg cgtcgcagct tccagaccca gaaggccaag
     1021 gccgacgccg accttctgaa acagggcgag gagatcgcca acctgacgaa agagcgcgac
     1081 aagtgggtca ccaaatacga cgacgtcgcg gccaaactgg aaaagtacct gccgccgagc
     1141 aagactggca cgaccgccgg caaggccagt ccttacacgg tgtggtactg ggtcggcggc
     1201 gcggtcctgg ccgccctggc cctcgtctgc ggcgttttct ggatcggcgg ccagatgagc
     1261 gccgagaaga ccgaggaaag cgaagaactc aggcctgaag aaccgaagaa ccagtaagcc
     1321 ggtcagtcgg tgggtcggtc ggatccacca accgaccacc gacccaccga ccaccgacca
     1381 accgaccacc gaccaccgac caaccgaccc cgtggacgct tcctcccgca tcgcccgact
     1441 caacgccgac cgcgagctat tggcggcgct cgatgcggcc agctcgatct tcagcctgga
     1501 agtgacgggc gatccgcccg accgctacac gctcacgttc cggggcaagg gtctgggccg
     1561 cgacgcggcg gccagctccg acgtcacggt catcgaattg caccagatcg acctgcggat
     1621 gccctatgcc tatcctgcca gcccgcccga catccgctgg atcacgccgc tgtggcaccc
     1681 caacgtctcg ttcagcggct tcgtgaaccc ggcggacatc ggcctggtct ggaccagcga
     1741 gatgccgctg gacctcgtct gcgagcggct gtgggacgtg gcccgcggcc agttcctgaa
     1801 cctcaataag gccacgaact acgctgccaa gaactggttc gaaaaggagt gcacctatca
     1861 actgccgctc gacccgcgga gcctgcgcga cggcggcggc gcccgcagct cttcgaacgt
     1921 cgtccgctac gagcggcggg ccggccaggg agtgcaattc gccggcgcgg ccgcaatcgg
     1981 cgacgtgatg ttcatcgacg agaacacgcc cacgccgcaa atgcccgccc ggcagcccta
     2041 catcccctcc gcccgccgcc gcccacgctc cggcgacgac gtgatgtata tcgggccaga
     2101 gtaaatgcgg aatgcggaat gacagcagaa gcgatgcctg ctattccgca ttcccaactc
     2161 cgcattccgc attcctcggt tgcactccat cctgcggcga ctcaaaatcg agtgacggtc
     2221 tcgattctgt aaaggatgcc tgccgtggag tgcttcgtca tcgccgtgat cctgttcggc
     2281 agcgtgatcg cgatggcgat cgcgacgctg gccagcgccc gcggcggcgg ccagtggaac
     2341 gaggctttca agcacgtcgc ccgccgcttc cacggcacgc tccaccccgg cggctggttc
     2401 tcgcagcccg cggtctggat gcaacacggc gagtcgcacg cccgtttgac cgtgaccacg
     2461 ctgcgcggct ccggcggcga gcggctgctg caaatgacca tccagcagcg cgacctgcga
     2521 acgcagtgcg aaatctacta tcaccaatcg cggcccgccc tcgtgccgcc gacccgcggc
     2581 ctgttcccgg tcgagttcga ctgggaggag ttccgccgcc gctggatcgt caaggccgcc
     2641 gacggcgacg aggttcgcca cctgctcacc gacggcgtgc ggctggcgat cgagcaactc
     2701 tggcggcacc cgctgccggg cgagacgacg atcagcctct cgcccagctg gctcgtcgtt
     2761 cgcaagctct ggaactcgcc tcgcggcatc gacatcgagg agttcgccga gcggacgctg
     2821 atgctgtcgg accagctgca tctggccgcc gcgtccggca tcgagttcgt cgtgagcgat
     2881 cagccgcaac tgctcgacga ggcccgctgc ggcgtctgcg gtgattcgct ggcccagtcg
     2941 gtcgtcgtct gccgccgctg caatacgccg caccaccgcg actgctggga ctatggcggc
     3001 ggctgcgcga cctacggctg cggcggccgc gaatgcttca ttcccggcgt cgccccgctc
     3061 gccgccccgc actgggacgc ggcgacgcag ccgacggagc ggccggcgaa gccgcgctga
     3121 actaggcgtc cgcgggcgtg gcgtcggtcg gggcttccag gccgtcgatg aagctggcca
     3181 gcgagcggca gcggtagggc tgctggagct tacggacggc tttggtctcg atctggcgga
     3241 cgcgttcgcg ggtcacgctg aagatcttgc cgacttcttc cagcgtgtag gagtagccgt
     3301 cggtcaggcc gtaccgcagg cggaggattt cccgctcgcg gtagttcagg ccctgcatcg
     3361 cctcgtcgat ccggttcttc agggcctgct ggtgcgtgtc gtagagcggg tcgtcgtcgc
     3421 ggtgatcggc caggaactcg ccaaaatagc tgtcctcgtg gtcgcccacc ggctgatcga
     3481 gcgagagcgg ctgccgcgac atcttgagga tgcagcgggc gtcttcgagc gagaggccgg
     3541 cccggcgggc ggtctcttcg gccgtcggct cgcggcccag ctcctgcagc agcgtccggt
     3601 tgaccgaccg cacgcggctc atcgtgtcga tcatgtgcac cggcacgcgg atcgtgcggc
     3661 tctgatcggc gatggcccgg gtgatcgcct ggcgaatcca ccaggtggcg taggtgctga
     3721 acttgtagcc gcgggcgtat tcgaatttat ccacggcccg catcaggccc gtgttcccct
     3781 cctgaatcag gtcgaggaac gaaaggccgc ggttgcggta tttcttggcg atgctgacta
     3841 ccagccgcag gttgccggcc gagaggtcgc gcttggccgc gtcatactcc tcgcggtaga
     3901 tctcggtccg ctcgattcgc cgccgcagcg tggcgggcgt ttcgagcgag atccgcatca
     3961 gataatacag ctccttgcgc agctcgggct cggtgcggcc gccggagagg ccgaccacgc
     4021 gggcctcgcg aatctgcatc ttcagcgtct tcatccgcag caggatttca gccagcttgc
     4081 cgaacaacgg ctgcagccgg ttgttgcgga ggttcatctc ctcgaccagg cggacggcct
     4141 tgttgcggcg gcgggtgagg cgggcccagg cagcccggcg ctgcttcatc gggaattgct
     4201 tgctgacggc gatgcggaaa tcgcggtggt tctggttgag caggtggcgg agcgtagcca
     4261 cgttcggcca gatccgcttc atgatccgct tcttctcggt cgtgttggtg accgagactt
     4321 cgatcgtgcg gtccagacgc aattggccgt cgcggacccg ctccagaaat tcgacggccc
     4381 cttgcaggac gtagtcggtg gccagcatcg agtggcgata gcggagccgc gtccgctcga
     4441 taacctgggc ggaagcgatt tcctgggccc ggttgaggag cggaatctgg cccatctgca
     4501 tcaggtacat gcggaccggg tcgtcggtcg catcgacgct ggcctcgtcg tcgccgtgca
     4561 gatcctcctc ttcctcctcg tcgtctaagc ccggctcgtt ctcttcctcg gcagtcgcga
     4621 aaccggggcc ggcttcgtcc tgatccgcga agccgtcatc gtgcgccagg ccgtggccat
     4681 tgacggactt gacctttttc gcggcggtcg ccggcttgcg gaagttcttg gccatcaggt
     4741 cgattgctcc gggagtgtcg cgaacgcgtg ctgtgggtgg accaaaagca aggccgatac
     4801 cagcagccgc gagcggcggc gagaagcggg cctaaggact gactcggtca ggacttgcgt
     4861 ccggcgtgct cggatgagcg gtgagccgcc gcgttgccag acagcaacgc ttgttgccag
     4921 agggcatcca gtgaatcgta gaagcaacag tgagcaagag aaggcgcagg gagcgtagaa
     4981 atccaggccg aggctgccag tcttgccgtt tttgacgccg gtagcgatgg tgaaggaatg
     5041 acgcggtccg acgaaggcaa tggctcgtgg ctggcacggc tccgcccgtg gctggggcca
     5101 ctggtggcgg tcgtcgtctt cgcggcggcg attgtcctgc tgcggcagga gctgaagcat
     5161 cacagctggc gccagatcat gcgcgaggtc ggcgccgtgc cgccgcagcg gatcgccctg
     5221 gcgatcggcc tgaccgcgct caactacgtg gttctcagcg gctacgacgc cctggccgtg
     5281 cggtacgtga agcacccgct gcggcccacg cagattctgc tcgggtcgtt cgtcggctat
     5341 gcgatgagcc acaacctggg ctggatgctc ggcggcacga catcgcggta ccggctgtat
     5401 tcgacctggg gcttctcggc ggtcgagatc atcaagctgt ttgcgatcct ggggctcacc
     5461 ttctggtcgg gctattgctt tctggccggc gtcgtgtttc tgctcgatcc gctgccgatg
     5521 ccaccgcaat tgcacctgcc cctggccagc acgttctggc tggggccgat tctgctggga
     5581 atgctcgcgg tgtatctggt cgcgtgcgcg atcggccggc cgatcgttat ccgcggctgg
     5641 aggatcgagc tgccggcgct gacgctcgcg ctgggccaga tcgccgtggc gtcgtgcgat
     5701 ttgctgctgg cttcggcggt gatgtacgtc ctgctgcccg cggggatggc gatcagctac
     5761 tggcggttcg ccaacgtggt gctgctggcg ctggcctcgg cgatcatcag ccacgtgccg
     5821 ggcggcgtcg gcgtgctgga actggtggtg gtcaagctgg tagcgcctga cgatcccgcg
     5881 gccctcgtcg gcgggctgtt ggcattccgc gccatctatt acctgctgcc gctaatgact
     5941 gcgctggggc tgctgggcgg gcacgagctg atcgttcatc gcgagcaggc ccacgggctg
     6001 gtcactcggc tgggccagtt ctttccgcat atcgccccgc ggctgttggc cttcgcctgt
     6061 ttcatcgccg gcgtcgtgct gctgttatcc ggggtgacgc ccggcgagga cagccggctg
     6121 gattggctag agcggttcct gccgttgggc gtgctggagg cttcgcattt tctgggcagc
     6181 gtcgtgggcg tcgtactgct gttgctggcc cgcggcctgc agcggcgatt ggattcggcc
     6241 tattggctgg caatcgtgct gctcgcggcc ggcatcgccg cgtcgctgct caagggcttt
     6301 gatttcgaag aggctgtgta tctggcgctg atgctggcgg cgctgttgcc ctgccggcgg
     6361 cacttctacc gcaagagctc gctcttgtcc gagcggtttt cgccgcagtg gacggtggcc
     6421 gtcctgctgg tgctcgtttg tgctcttggg ctggcgctgt tcgcgtttga aagggttgag
     6481 tacagcaacg agctgtggtg gaagttcgcc ttcaccgcca gcgccccccg gacgctgcgg
     6541 gcgctggccg gggtgggaat tgctgtgttc tcgatcgccc tggcgcgcct cttgcgcgcc
     6601 aagcccaaac cgccggccgc gccgacgccg gcggagctgg tcgaagccac ccagcttgcc
     6661 cgccgctcgc ccaggactta tgcgcagctt gtcggcctcg gcgacaagtc gctgctgttc
     6721 agcgaggacc ggcagggatt cctgatgttc gcggaagagg gccgctcgtg ggtggtgctg
     6781 ggcgatccgg tcggccctga tgaagtcgct gaagagctgg cctgggagtt tcgcgaactg
     6841 tgcgacgccg gcggccgctg gcccgtgttc tatcaggtcg aaacggagcg gctgccggtc
     6901 tatatcgagc tgggcctgtc gatcctgaag ctgggcgagg aggcccgcgt gccgctggcg
     6961 ggcttcagcc tggagggtcg cgaacggaag tcgctgcggc acacctgcac gaggctcgag
     7021 cgcgagggct gtacgctgga ggtcgtcgag ccgccgctgg cggagccgct gctcaatgag
     7081 ctggcggcga tttccgatga ctggctgcgc cggaagaaca cgcgcgagaa gggattttcg
     7141 ctgggcttct tcgagcgcgg ctacgtgcgg caatccgccg tggcgctggt gcggcagtcg
     7201 gaccgggtcg tcgcgttcgc gaacgtcctg cgcggcggtg acaaggagga gctgtcggtc
     7261 gatttgatgc ggtttgccga cggcgcgccc ggcggcgcga tcgattacct ctttgcccag
     7321 ctcatgctcc ggggccacga ggaaagctac cgctggttca atctgggcgt cgccccgctg
     7381 gctggggtcg agggtcactc gctcgctccg gcgtggaacc gcttcgccga gatcgtctat
     7441 aggcacggcg agcacttcta caactttcaa ggcctgcgcg attacaagga caagtttgat
     7501 cctgtctgga cacccaagtt cctcgcctgt cccggcggcc tccgcctgcc catgatcctc
     7561 accaacctgg ccacactgat ctccggcggg ccgaaggggc tggtagggaa gtagctttca
     7621 tttgacattc ccgacgtatg ctccgatgat gcgcggcagg gcaagattcc ttgccggcca
     7681 gcgaattact ggcaaaagca actggtggtt tggcattggt gaccagtttg caaggggcgt
     7741 agttatggtc gcgttaacct ggttcttcat cttctgcgcg gtcgtcggcg gcacggtgct
     7801 gatttgccag ttcgtgctga cggtgctggg cctctcgggc gaccacgggc tcgatgcggg
     7861 gcacgatctt accggcgatg ccggtcacga cgtgcacgcc gtcggcgcgc acggcgcgga
     7921 gggcgacggc cacgaccagg ccggccatca cggctcgtcc tggctgttcg cgatgatctc
     7981 gttccgcacg ctggtcgccg ccgccgcgtt cttcgggctg gttgggctga ccgcgcagtc
     8041 ggccggccag cccgtgggtg tgcagctgct gctggccgcc ctggccggat tcggcgcaat
     8101 gtatggcgta cactggctgg tccgcacgat gggccggctg ggcgaggacg gcacgcagcg
     8161 cgtgcaaggc gcggtggggc aggaaggcac cgtttacgtg cccatcccgc cgggcaagag
     8221 ccaggccggc aagattcagc tcaaactgca aaaccgcctc gtcgagtacg aagcggtgac
     8281 gaataacccc gagcggctgg cgaccggcac gcgcgttcgc gtcgtggccg tcgccggcaa
     8341 ccagctcgaa gtcgaaccgc tgaaagtttg agttcaaaga tcaaagctca ggttcaaagt
     8401 tggctgcttc ccaactttga accttgaact ctggaccttg atccgtaacg ggagtgatgc
     8461 tgtgacatct tcgatcttgt ttgcacaaat gccggccgag gtgatgggca tgctcatcgc
     8521 caccaccttt gccctgcttg gcattctggt agtgggcctg ctcatcgtct tcgttcgggg
     8581 cttcaaacgc tgcccgagca accgcgtgct ggtcatctat ggccgcaccg gcaaggccgg
     8641 ggccaccaag tgcgtgcatg gcggcgccga tttcgtctgg cccctgatcc aggactatgc
     8701 ctacttgagc ctggagccga tccagatcga ggtgccgctc cgcggggcgc tttcgaccga
     8761 gaatatccgc gtcaacgtgc ccagcgtgtt cacggtcgcg atcgatacca agcccgaagt
     8821 gatgcagaac gcggctgtcc gcctgctcgg cctgtcggtg ccggaaatcc gcaagcaggc
     8881 cgaggaaatc atcttcggcc agctgcggca agtgatcgct tcgatgggca tcgaagagat
     8941 caaccgcgac cgcgacaagt tcctccagca cattcagcat tcgctggagc cggagctggc
     9001 gaagatcggc ctgcagctga tcaacgtcaa catcacggac atcacggacg agtcggggta
     9061 tatcgacgcc atcggccaga aggcggcctc gctggcgatc cagcaggccc gcggcgacgt
     9121 ggccgacaac gaaaagatgg gcgagacccg cgtggcctcg gccgagcgcg acaagtcggt
     9181 gcaggtcgcc aacgcccgca aggagcagtc gataggcacc cgcgaggccc agcgcgaaca
     9241 ggccgtgcgg atcgccacgc tggaaaagga gcagtcgatc ggcgaaaaga ccgcggcctt
     9301 cgagaaggac tcgcaggtca agcaggccga acagcagatg cgcgtgcagg tggccgaggc
     9361 cgacgccggc gcgatcgacg gcgaaaacca ggccgctgcc cgcgtcgctg cctcgcaagc
     9421 cgagctggcg atcaagaagg ccgaagccta cgagcgcggc gaaggccgcc gcaagcaggc
     9481 cgaggcgcac gtcatggaga ttcagaacca tgccctggcc aaggccgccc tagccgaggc
     9541 ggagcgggtc gaagccgagc agcgggccaa gctggaagcc cccgccaaag cccagaaggc
     9601 ccgcgtggtc gtcgatgccg aagcggccgc cgaccagcgc cgcatcatgg ccaaggcgga
     9661 agccgacgcg atctacctca agctcgaagc cgaggcccgc ggccagtacg aaatcctggc
     9721 caagaagggc gacggcctca agcagatcgt cgaggcctgc ggcggctcca cgcaagcctt
     9781 ccagatgctg atgctggaac acctcgacaa cctggccgac tgctcggccc gcgcgatctc
     9841 gaacatcaag ttcgacaaag tcgtcgtctg ggaaaacggc gggtccaacg gccgcggcag
     9901 cacggcggac ttcctcagca gcatggccaa gaccatgccg ccgatgatgc aggtgatgcg
     9961 cgacatcggc ggcatcgagc tgcctgaatc gctggtcaag ctcggcggcg acgctaccaa
    10021 cagcggtgtg gccgtcgcca ccaacggcaa tgcccctccc gccgcggctg gcaaggacaa
    10081 gggcaaggcg tgagaactcc aagtagctta cacagagaca cggaggcaca gagggtacaa
    10141 tgcgggaatg ttctcgcgac ccccttacgt gctgattggc ttgctgacgg gcatctttat
    10201 tggctggttg attccgagtc tggatagccc gtggcagttc gatttgctga cctacgggtt
    10261 cgtcggagct aggcgaaaga acccagcaac acgtttcgac ccatgaccca agtcatctgc
    10321 tctgtgcacc ctgtgcctct gtgtgagatt tgaccgggcc acacctactg ttggctttct
    10381 tccccgcgtt gtcttagaat gcgggacgca acgcgtcgga agccgtgcgc ctttccgcat
    10441 ggccgatggg ggcggcgtgt ggaaggagta ttcgtcgcac gaggaccgcg cccatgccca
    10501 gccaacgccg tctcggcatc tccgccagtt gcctggcgaa ctggctcgcg gtgttgccgg
    10561 ttctcttgcc gctgcccgtt gcggctgccg acctggctga gctcacacaa ctctttcgcg
    10621 ccggcaagta cgccgagtgc gtcaccggcg cagccgccga aatcgaaaac gcccgctaca
    10681 gcgaaaacct ccggctgctg aagctgcggg ccgagatgga gttgggccgc tacggcgacg
    10741 ctctgaagac gctcgacgcg gccctcaagg attttccgac cagcattcac cttcgctggg
    10801 tcggccgcag cgtttgccgg ctcaatcagc agccggagcg gtccgccgcc ttcgagacgc
    10861 agatcgccgc cctgctcaag cagtccccct ggcaatacag cgacgccgtc aaccaggtcg
    10921 ttgccggccg gttctatctg agccagggag tggaccccaa ggtggtgctc aattcgtcgt
    10981 acgtggaagt ccggaaacgg caaccgacct atgtcgacgt ttacctggca atcggcgacc
    11041 tgg
//
LOCUS       DASRJB010000231         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2033407, whole genome shotgun sequence.
ACCESSION   DASRJB010000231 DASRJB010000000
VERSION     DASRJB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033407"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFB80_06390"
     CDS             complement(<1..741)
                     /locus_tag="VFB80_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33428.1"
                     /translation="MKLTLLSQLTILCSAIVSSAAEPAVINVWPGKAPGETKELPAEG
                     LQAPKAKEVKAVKRVQNVSTPTLTVFQPPAEQRNGAAVVIAPGGGYNILAWDLEGTEV
                     AEWLNSLGVTAFVLKYRVPKREGDTANKLPLMDAQRAMSLVRSKASDDGIDPARIGML
                     GFSAGGNLTAATCLKFAERQYDKLDAVDDVSCRPDFGVLIYPAYLVDNEGKLKPEYQP
                     TKETPPMFFAHALNDGVTPDSSIALTRAL"
     gene            complement(738..1211)
                     /locus_tag="VFB80_06395"
     CDS             complement(738..1211)
                     /locus_tag="VFB80_06395"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZN33429.1"
                     /translation="MIIWGSRSRHIPLGQGQFFCPRCQAQREYSHSRVARYFHLYFIP
                     LFEMGNLGEYVACLACGGEFGAEALNQHQPTQGEQLALATRRDLDGGTPLHMVKQKLI
                     NGGMQPQMAKQFLALAAESPLGHCPKCNFYYRHTVQVCSGCGGGLRTTKESKGIL"
     gene            complement(1213..1935)
                     /gene="deoC"
                     /locus_tag="VFB80_06400"
     CDS             complement(1213..1935)
                     /gene="deoC"
                     /locus_tag="VFB80_06400"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZN33430.1"
                     /translation="MNYSYSDLAKMIDHSLLNPTLTAADLEAGCRLALAYDVASVCIM
                     PYYLRRCAELLRGSSVMASTTIGFPHGGHTAAMKAAEARQALADGGQELDMVVNISQV
                     LSGAWDAVRQDIAGVIQAAHDAGAKVKVIFENCYLKEEHKIRLCQICGELGADWVKTS
                     TGYGTGGATIEDLMLMRKHAPPHVQVKAAGGVRDLDMLIKVRELGVSRCGASRTKEML
                     DECRRRLGQPAINAAGGPAQGY"
     gene            complement(2268..2669)
                     /locus_tag="VFB80_06405"
     CDS             complement(2268..2669)
                     /locus_tag="VFB80_06405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN33431.1"
                     /translation="MAAEFINPPSLCPTFGWTHVVTATAGKTIHISGQVAVNERGEVV
                     GRGDLKRQTEQAFENLRLALAAAGATWNDVIVTRLYVVGLKPEHVPIIREVRSRYVSS
                     QHPPASTLVGVSALVGADWLIEIEAAAHVEL"
     gene            2876..3670
                     /locus_tag="VFB80_06410"
     CDS             2876..3670
                     /locus_tag="VFB80_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZN33432.1"
                     /translation="MRLGFGWLAVIVICMSPVAAQAALFSFDSPAEVVLSPTQAPNVW
                     YTDRYPPAIFASGQNGGGRTGVLQQNINAEDFDRDPPFDSAFYNTQGRKYDLAPGTQG
                     LVIELYVPSSWDALSQIDGRLASFWATGFNDANIVTDFFPIIEFNTSRPGLGDNGFRV
                     WDSNTGWFNVGGFSGFNQWYQLGFVLEGGQERFFVNGSLIGSVNDPLTTQLGNVILQG
                     YNSGNSYNISWDNLSVVPEPATLPAFALLLAGIGCGALRRRKQRRS"
     gene            complement(3747..6179)
                     /locus_tag="VFB80_06415"
     CDS             complement(3747..6179)
                     /locus_tag="VFB80_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33433.1"
                     /translation="METTLPPGATITPIADATLPPPAAGSTLRTPALADVTFDPNAPQ
                     AQAYREAAQAAPPLASGSIVTGASFGHYQLLEPIAKGGMGIVYKARQRNLNRIVAIKM
                     ILAGQFADQADIDRFYAEAEAAATLRHPNIVAIHEIGEVQGQHFFSMDYVDGPSLSNL
                     VHESPLPPRRAAELVRTIAETMQFAHDSGIVHRDLKPSNVLLDKKQRPLITDFGLAKQ
                     VAGQSQMTIAGAVIGTPSYMPPEQAAGDGANVGPWSDLYSLGAILYETLTGKPPFRAA
                     TPFETIRQVLDSEPLSPRLLNPGVPRDLETICLKCLQKERSRRYATAQELADELSRYL
                     RGEPIQARPIGQAARFWRLCRRNPLTASAIAAAVLMLAATAGIMAVAYVQTSAALNQS
                     EQSLREALGAVNDLFTTVSEDTLLNQPGMQPLRKDLLNKALTYYQRFLKQRANDPRVQ
                     DELAAAYFRIGLITEVLQSPDEALPAYETARQMQQRQLAARPSDPARLEALGNTLSAL
                     GALWTRKNDYAAALKEFQAAVDVRSKLAGQVAASSDYQRALANSHMNIGLVHYNAALA
                     AETDEEEDAGLERARREFGRAQDVRAAALAREPANPKLRRDLGKGHYNLGNLEWAAKR
                     NAPAARQFQSAADQFERLAKAQPGDLENQKLLAICYRFLGDICSQDDPEEARGWYRLA
                     SERLEPLAEQNPGVIDYQSERAGLSMNVFLLERDAGQAAAARAALEQAQGILAPLVQR
                     FPAVPRYRRDLAVTLRELAVLKDKAGETRAAAADLMESTRILKSLTAKYPSEMEYAVQ
                     LEQTEAVKLAAP"
BASE COUNT         1016 a         2173 c         2123 g         1218 t
ORIGIN      
        1 gagcgctcgc gtcagggcga tgctgctgtc gggcgtcacg ccatcgttca gggcgtgggc
       61 gaagaacatc ggcggcgttt ccttcgtcgg ctggtactcg ggcttgagct tcccctcgtt
      121 atcgacgagg taggccgggt agatcagcac gccgaaatcc ggccggcacg agacgtcatc
      181 cacggcgtcg agcttgtcgt attgccgctc agcgaacttc aggcaggtcg ccgcggtcaa
      241 gttgccgccc gccgagaatc ccagcatgcc gatccgcgcg gggtcaattc catcgtcgct
      301 ggccttgctc cgcaccaggc tcatcgcccg ctgcgcgtcc atcagcggca gcttgttcgc
      361 cgtgtcgccc tcgcgtttgg gcacccggta cttcagcacg aaggccgtca cgcccaggga
      421 gttgagccac tcggcgactt cggtcccctc caggtcccag gcgagaatgt tgtacccgcc
      481 gcccggcgcg atcacgaccg ccgcaccatt gcgctgctcc gcaggaggct gaaataccgt
      541 cagcgtcggc gtgctcacgt tctgcacgcg cttgaccgcc ttgacctctt tcgccttcgg
      601 cgcctgcagc ccctcggccg gaagctcctt cgtctccccc ggcgctttcc ccggccagac
      661 attgatcacc gccggctcgg cggcgctgga cacgatggcg gagcaaagaa tcgtcaactg
      721 cgagagcagc gtcagcttca tagaatcccc ttgctttcct tggtcgttcg caaaccgcca
      781 ccgcagccgg agcacacctg caccgtgtgg cggtagtaaa agttgcactt cgggcaatga
      841 cccagcggcg actcggccgc cagcgccagg aactgctttg ccatttgcgg ctgcatcccg
      901 ccgttgatca gcttttgctt gaccatgtgc agcggagttc ccccatcgag gtcgcgtcgc
      961 gtggcaagcg cgagttgctc tccctgcgtc ggctgatgct ggttcagcgc ctcggcgccg
     1021 aattccccgc cgcacgccag gcaagcaacg tactcgccca gatttcccat ctcaaacagc
     1081 ggaatgaaat acaagtggaa gtatcgcgcc acgcggctgt gcgaatactc gcgttgggcc
     1141 tggcagcggg gacagaaaaa ctgcccctgt ccgagcggaa tatggcggct gcggctgccc
     1201 cagatgatca cgctaatacc cctgcgccgg cccgccggcc gcgttaatgg ccggctggcc
     1261 cagccggcgg cggcattcgt cgagcatctc cttggtgcgg ctagcgccgc agcggctgac
     1321 gcccagctcg cggaccttga tcagcatgtc gaggtcgcgg acgccgccgg ccgctttgac
     1381 ctgcacgtgg ggcggcgcgt gcttccgcat cagcatcaaa tcctcgatcg tcgccccgcc
     1441 ggtgccatag ccggtgctgg ttttcaccca gtcggcgccg agctcgccgc agatctggca
     1501 gaggcggatt ttatgctcct ccttgagata gcagttctcg aaaatcacct tcaccttcgc
     1561 gccggcgtcg tgcgcggcct gaatcacgcc ggcgatgtcc tgccgcaccg cgtcccacgc
     1621 gccgctcagc acctggctga tgttgacgac catgtccagc tcctggccgc cgtcggccag
     1681 cgcctgacgg gcttcggcgg ctttcatcgc cgccgtgtgc ccgccgtgcg ggaagccgat
     1741 cgtcgtgctg gccatcaccg agctgccccg caggagctcc gcgcaccgcc gcaggtagta
     1801 cggcatgatg cagacgctcg ccacgtcgta ggccagcgcc agcctgcacc ccgcctccaa
     1861 atccgccgcc gtcagcgtcg gattcagcag cgagtgatcg atcatcttcg ccaggtcgga
     1921 atagctgtag ttcatggctt tggagagtgt actgaatgaa ggcgggctgt gcccaccaat
     1981 gtctgcgagg cggatagcac ctgcccgcga gatttcgcag agccaaccgc atgacgtccc
     2041 gaattatgag ccccctggtt aggcagggct gaattggccc catcgggcca caactcacgg
     2101 cgcgccatcg acttagggcg gatttttcgc gagctcttaa gtactactta ttacggttag
     2161 agaactcggt ttggaccgac ccactagcag ggctccagca gcgaccggtt gccaaaaagg
     2221 ccgaccgatt gccaaaaaga acttccttgc aaataccata ccaatgttca caattccacg
     2281 tgcgcggccg cttcgatctc gatcagccag tccgcgccga cgagagccga cacgccgacg
     2341 agcgtgctcg ccggcggatg ctgggacgag acatagcggc tgcggacttc gcgtatgatc
     2401 ggcacgtgct cgggcttgag gcccacgaca tagagccggg tgacgatgac gtcgttccag
     2461 gtcgcgccgg cggcagcgag agctagccgc aggttctcga aggcctgctc ggtctgccgc
     2521 ttgaggtcgc cgcggccgac gacctcgccg cgctcgttga cggccacctg gccgctgatg
     2581 tggatcgtct tgccggcagt ggcggtgacc acatgcgtcc agccgaacgt cgggcagagc
     2641 gaaggtggat tgatgaattc ggcagccatg tattgcgctc caaattgtta ggtattcggc
     2701 actttcagcg gatcttaaag accggagttg ggtgtcagcg tttgtcggca aaagattttt
     2761 taaatggatg taaccattga ctgctcccat gcgatttagc cggtcggaag ggaagaggct
     2821 ccctccgcaa tgccggctgg ccggcgcgat cgatctttga actgggaggc aggcaatgag
     2881 gctcgggttt ggatggctcg cggtaatagt gatttgcatg tcgccggtgg ccgcacaggc
     2941 ggccctgttt tcgttcgaca gcccggcgga ggtggtgctg agcccgacgc aagcgccgaa
     3001 cgtgtggtac accgaccgct atccaccggc gatcttcgcc agcgggcaga acggcggcgg
     3061 gcggacgggc gtgctgcagc aaaacatcaa cgctgaagac ttcgatcgcg atcctccctt
     3121 cgactccgcg ttctataaca cccagggtcg caagtacgac ctggctcccg gcacccaggg
     3181 tctggtcatc gaactgtacg tgccctcgtc gtgggacgcg ctgagccaga tcgacggccg
     3241 gctggccagc ttctgggcca cgggctttaa cgacgccaac atcgtcaccg acttctttcc
     3301 catcatcgaa ttcaacacca gccggcccgg cctcggcgac aacggcttcc gcgtctggga
     3361 cagcaacacc ggctggttca acgtcggcgg cttcagtggc ttcaaccagt ggtatcaact
     3421 cggcttcgtg ctggagggcg gccaggagcg ctttttcgtg aacggcagcc tgatcggcag
     3481 cgtcaacgat ccgctgacga cgcaattggg caacgtcatc ttgcagggct acaactccgg
     3541 aaactcctac aacatctcgt gggacaacct gagcgtcgtg cccgagccgg caacgcttcc
     3601 cgccttcgcc ttgctcttgg cgggcatcgg ctgcggggcc ctgcgccggc ggaagcagcg
     3661 ccgttcgtag ccgacgctgc cagcgtcgga gtgtgaattg tgattggacc attgcctgcc
     3721 agcctggacg acgctggcag cgtcgtctac ggcgccgcca gcttgaccgc ctcggtctgc
     3781 tcgagctgaa ctgcatattc catctcgctg ggatacttgg ccgtcaggct cttcaggatt
     3841 cgcgtcgatt ccatcaggtc ggcggcggcg gctcgcgttt cgcccgcctt gtccttcagc
     3901 acggccagtt cgcgcagcgt gaccgccagg tcgcgccgat agcggggcac ggcgggaaag
     3961 cgctgcacaa gcggcgcgag gatgccctgc gcctgctcca gcgccgctcg cgcggctgcc
     4021 gcctgacccg cgtcgcgctc cagcaggaac acgttcatcg agaggcccgc ccgctcgctc
     4081 tgatagtcga tcacccccgg attctgctcc gccagcggct ccagccgctc gctggccagg
     4141 cgataccagc cgcgggcctc ctccggatca tcctggctgc agatgtcgcc aagaaagcgg
     4201 taacagatcg ccagcagctt ttggttctcc aggtcgcccg gctgggcctt cgccagccgc
     4261 tcgaactggt cggccgcgct ctggaattgc ctggcggccg gcgcattgcg tttggcggcc
     4321 cattcgagat tgccgaggtt gtagtgaccc tttcccagat cgcggcggag cttggggttc
     4381 gccggctcgc gtgccagggc cgccgcgcgg acgtcctgcg cccggccgaa ctcgcggcgg
     4441 gcccgctcca ggccggcgtc ttcttcctcg tccgtttcgg cggccagtgc cgcgttgtag
     4501 tgcaccagtc cgatattcat gtgcgaattc gccagggccc gctgatagtc gctgctggcg
     4561 gcgacttgcc cggccagttt gctgcggaca tccaccgcgg cttgaaattc cttgagcgca
     4621 gcggcgtaat cgttcttcct cgtccagagg gcgcccagcg cgctcagcgt gttgcccagc
     4681 gcctcgagcc gcgccggatc gctgggccgc gccgccagtt ggcgctgctg catctggcgg
     4741 gcggtctcat aagccggcag cgcctcgtcg ggcgactgca gcacttcggt gatcagcccg
     4801 atccgaaaat aggccgccgc caattcatcc tgcactcgcg ggtcgttggc ccgctgcttc
     4861 aggaaccgct ggtaatacgt cagcgccttg ttcaagaggt ccttccgcag cggctgcatg
     4921 cccggctgat tgagcagcgt gtcttcgctg acggtcgtaa acaggtcgtt gaccgcgccg
     4981 agcgcctcgc gaaggctctg ctccgactgg ttcaaggccg cggaagtctg cacgtaggcg
     5041 accgccatga tgccggccgt cgcggcgagc atcagcacgg ccgcagcgat ggccgacgcc
     5101 gtgagcggat tgcgccggca gagccgccag aagcgagcgg cctggccgat cggccgggcc
     5161 tggatcggct cgccgcgcag gtagcggctc aattcgtcgg ccagctcctg ggccgtggcg
     5221 tatcgccgcg aacgctcctt ctgcaggcac ttgaggcaga tcgtctccag gtcccgcggc
     5281 acgccagggt tcaacagccg cgggctgagc ggctcgctgt cgagcacctg gcggatggtc
     5341 tcgaaggggg tcgccgcgcg gaagggcggc ttgccggtga gcgtttcgta gaggatcgcg
     5401 ccgagcgaat acaagtcgct ccacgggccg acgttcgccc cgtcgccggc ggcctgctcg
     5461 ggcggcatat agctgggcgt gccgattacc gcgccggcga tcgtcatctg cgactggccg
     5521 gcgacctgct tggccaggcc gaagtcggtg atcagcggcc gctgcttctt gtcgagcagg
     5581 acgttgctgg gcttgaggtc gcggtggacg atgccgctgt cgtgggcgaa ttgcatcgtc
     5641 tcggcgatcg tgcggaccag ctcggccgcc cgccgcggcg gcagcgggct ttcgtgcacg
     5701 aggttcgaca ggctcggccc gtccacgtaa tccatcgaga agaagtgctg cccctgcacc
     5761 tcgccgatct cgtgaatcgc gacgatgttc ggatgccgga gggtggcggc ggcctcggcc
     5821 tcggcgtaga accggtcgat gtcggcctgg tcggcgaatt gcccggccag aatcatcttg
     5881 atcgccacga tccggttgag gttccgctgc cgggccttgt agacgatccc catcccgccc
     5941 ttggcgatcg gctccagcag ctggtagtgg ccgaacgaag ccccggtgac gatcgacccg
     6001 cttgcgagag gcggcgccgc ttgggcggcc tcgcggtagg cctgggcctg gggggcgttc
     6061 gggtcgaaag tgacgtcggc cagcgccggc gtccgcagcg tggagccggc tgccggcggc
     6121 ggcagcgtcg catcggcgat tggcgtgatc gtcgcaccag gaggcaaggt ggtttccatc
     6181 ccggtcggcg ggccaggcgg cggcagtgtc gctggcggat cgaccgggag cggcgcgggc
     6241 ggcaaatctc ctgcggcggc cggcgcctcg cgcggagcac cctgcggcgc gggctctggc
     6301 gcctttttcc gtggcgcaag cgccctatcg ccgctgccgc tggtcgtttt atccgagtta
     6361 cccatgcggg cgcgatttta gcacagcgtg cacgattttg acgcaaggag agggggcgtg
     6421 gtggcgcagc ggaagccggc tggcggatgg attctgacca ggcgacgcgc catacttctc
     6481 tgtgcctgct gtcttctttg cgcctctgct cctttgcgcc tttgcgtcaa
//
LOCUS       DASRJB010000232         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2604528, whole genome shotgun sequence.
ACCESSION   DASRJB010000232 DASRJB010000000
VERSION     DASRJB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604528"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VFB80_06420"
     CDS             <1..1431
                     /locus_tag="VFB80_06420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33434.1"
                     /translation="VDIGRLVAVLGPLPCPAACEIIRQAALGLAHVHACGLVHRDLKP
                     SNLMLASDGVVKILDLGLARLREGQPAGDDSQTQSGYLLGTADYVSPEQLHDPHDADV
                     RSDLYSLGCTFYKLLAGQAPYCGTDPGSIGKKLDAHRYAAIPSVRSLRPEIPEEVDAI
                     LARLLAKEPENRFQQPADLAESLAPWTADAELAALCRKCGGRAEIDEPLRLPPSIAPE
                     DSTRPEGQTPTPHSALVPQRRRRWPLAVAAAGGLAAILVIAWIVANWDRGDKTSGGGR
                     AISGLDAIPTELPLPDAAAVNSLQVNQRMQTFDASAQSMQLVRLGELERGKGSITFDL
                     QFVADYGDAGLFLGYHEDLIQGHLWGIADFVYLATLPRGTKEKFFAFKHYQAQVRPGN
                     RDLYAIAESPEVRLPEGDRKFRLVVDFAPQGLTAIKLNGQPYPTLLKTGTLRQFQPES
                     IWGPWGIYNNGSTIRVSQPALTKEPL"
     gene            1428..2297
                     /locus_tag="VFB80_06425"
     CDS             1428..2297
                     /locus_tag="VFB80_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33435.1"
                     /translation="MTKRHMIREAVFGAVAINSPTSGIHCLDPSGSVAVEGTCDANTT
                     CLWWKLLDPSEDCATYNENPIQAGAAQIFPDSSGNWSGGSVSPGSPSDYGDRCVVIWC
                     LDEDSNQISMVVRNFTFLDPSNPLCGGSGSGSGGGSAMGTGAAGGFESAAAGHRRGRG
                     HGEPVIKESAPRAYRVYVPAGGLQLAEPLASLLSSAGDSLVGATLEFDPVRSSQRRAV
                     WRSDLRSAAWSLDVRRERGTLSATLTLFRLGRKSVRAPLVWCGAWCFRKQRLQLAGPR
                     GSRLPNLVIEPCG"
BASE COUNT          401 a          852 c          812 g          422 t
ORIGIN      
        1 gtcgatatcg gccggctggt cgcggtgctc ggtcccctgc cctgccccgc ggcatgcgag
       61 atcatccgcc aggcggccct tgggctggcg cacgttcacg cgtgcggcct ggtgcatcgc
      121 gacttgaagc cgtcgaattt gatgcttgcc tcggatggcg tcgtgaagat cctcgatctc
      181 gggctggcgc ggctgcgcga ggggcagccc gccggcgacg actcgcagac gcagtcgggc
      241 tatctgctgg gcacggccga ttacgtttcg cccgagcaac tgcacgaccc gcacgacgcc
      301 gacgtccgct cggacttgta cagcctgggc tgcacgttct acaagctgct tgccggccag
      361 gccccctact gcggcaccga tcccggcagc atcggcaaga agctcgacgc ccatcgctac
      421 gcggccattc cgtcggtccg ctcgctgcgg cccgaaatac cggaagaagt cgatgccatt
      481 ctggcccggt tgttggccaa ggaaccggaa aaccggtttc agcagcccgc ggatttggcc
      541 gagtcgctcg cgccttggac tgccgacgcc gagctcgcgg cgctgtgccg gaaatgcggc
      601 ggccgcgccg aaatcgacga accgctgcgg ctgccgccgt cgatagcccc cgaagacagc
      661 acccgccccg aaggccagac gccgaccccg cattcggcgc tcgtgccgca gcggcggcgg
      721 cgctggccgc tggccgtcgc agcggccggc ggtctggcgg cgatcctggt tattgcgtgg
      781 atcgttgcga actgggaccg cggcgataag acctcgggcg gcggccgagc gatctccggg
      841 ctggacgcaa ttccaacgga actgccgctg cccgacgctg ctgcggtcaa cagcctgcag
      901 gtcaatcagc ggatgcaaac gttcgatgcg tcggcccagt cgatgcaact cgtccgcctc
      961 ggagaactgg agcgcggcaa gggctcgatc accttcgact tgcagttcgt cgccgactac
     1021 ggcgacgccg gtttgttcct gggctaccat gaagacctca tccagggcca cttgtggggc
     1081 atcgccgact tcgtttatct cgccacgctt ccccgaggga ccaaggagaa gttcttcgcc
     1141 ttcaagcact accaggccca ggtccggccc ggcaaccgcg acctgtacgc catcgccgaa
     1201 agccccgaag tgcggctgcc cgaaggcgac cgcaagttcc ggctcgtcgt cgattttgcg
     1261 ccccaggggc tgacggcaat taagctgaat ggccagccct atcccaccct gctcaagacc
     1321 ggcacccttc gccagttcca acctgagagc atctggggcc catggggcat ctacaacaac
     1381 ggttccacca tccgcgtgtc tcaacctgct ttgacgaaag aaccgctatg acgaaacgac
     1441 acatgatccg cgaggccgtt tttggggctg tggcgatcaa cagcccgacg tccggcatcc
     1501 actgcctgga tccgagcggc agcgtggccg ttgagggcac ctgcgacgcg aacacgactt
     1561 gcctgtggtg gaagctcctc gaccccagcg aggactgtgc cacctacaac gagaacccca
     1621 tccaggcggg cgccgcccag atctttcccg atagctccgg caactggtcg gggggcagcg
     1681 tctcgcccgg cagccccagc gactatggcg accgctgcgt cgtcatctgg tgtctcgacg
     1741 aagacagcaa ccagatttcg atggtcgtcc gcaatttcac cttcctcgat cccagcaatc
     1801 cgctgtgcgg cggttcggga tcgggttccg gcggcggatc ggccatggga accggcgcag
     1861 ctgggggatt cgaatctgca gcggccggac atcgccgagg ccgcgggcat ggcgagccag
     1921 tcatcaagga gtcggccccg cgtgcttacc gcgtttacgt tcccgcgggc ggattacagc
     1981 tggccgagcc gctggccagc ctgctcagct cggcgggcga ctcgctggtg ggggcgacgc
     2041 tggagttcga cccggtccgt tcctcgcagc gccgcgccgt gtggcgcagc gatctgcgtt
     2101 ccgccgcctg gtcgctcgac gttcgccgcg agcgcggaac gctgtctgca acgctgactc
     2161 tcttccgcct gggcagaaag tcggtgcggg ccccgctcgt ctggtgcggc gcgtggtgct
     2221 tccgcaagca gcgcctgcag ctcgccggcc cccgcggcag ccgcctgccc aacctggtca
     2281 tcgaaccgtg cggataagtg gactgtttcg ttaacctgag ccggaggcga cgccggcacc
     2341 ggggaaacgg tcctattccg gcggcgtctg gtcgccgccg gatcgcgtga tgtaggccga
     2401 gagctgggtg agcgtgattg tgttcatcag catccggacc gagccgtcgc cgaagacgac
     2461 gttgcagccg gcggggtgaa agctgta
//
LOCUS       DASRJB010000233         6975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3733382, whole genome shotgun sequence.
ACCESSION   DASRJB010000233 DASRJB010000000
VERSION     DASRJB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733382"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFB80_06430"
     CDS             complement(<1..315)
                     /locus_tag="VFB80_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33436.1"
                     /translation="MSAEVPRRRGFVRTLTVAALVAAAGLAFGGWWFYGRKSAELGPK
                     LITTTVERRPYEFIVTELGSVENAATVELKCEVKSRGGGGERGGGGGGITIISVTPEG
                     THV"
     gene            651..1637
                     /locus_tag="VFB80_06435"
     CDS             651..1637
                     /locus_tag="VFB80_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33437.1"
                     /translation="MGPSPSARRDRRGFTLVELLVVIAIIGVLVALLLPAVQAAREAS
                     RRSKCQNHLKQWALAMHNFHDVYGNFPYFTQVTPRRHNWPPFVMPYLEQGNLVQGYDF
                     SINWYDGKNLPMTQVHLTVFNCPSDRPKPTWTDQTNYPSVRANYLVCYGNHVFGSATA
                     VGLGRGVFGMTTPPTPAGSNNFVPYKGRFADITDGTSNTLLMSEVIVARSDNNQGGGG
                     NWPSGDFRGHVWHDATSSNPTHCPNIFMTINGPNSTVPDNAMCGTIPNNDRAMPCVNG
                     ANTDRQNVARSRHPSGVNSCRADGSVQFVSNTIALASWRALGSMDAGEASND"
     gene            1749..2111
                     /locus_tag="VFB80_06440"
     CDS             1749..2111
                     /locus_tag="VFB80_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33438.1"
                     /translation="MICLPGCSPGKSTVSGTVTLDGQPVQSGAITFVKDEGGSMVREG
                     AVISDGKFEAAVPPGKYKLELTGQKVVGKRTQTGFDGKPETLDVTEQLFPEKFNTKTE
                     LMQEIKPGANPINLDLKS"
     gene            complement(2142..4697)
                     /locus_tag="VFB80_06445"
     CDS             complement(2142..4697)
                     /locus_tag="VFB80_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33439.1"
                     /translation="MLACSPPRPSRFLADCGELALKVRGQEHDGRVLRIRSPKCTIGS
                     ASGCTLRLMGAGVAPLHCWVLRGKYGTVVRQLRGRVSVNGAAAEESRLSPGDRFRVGE
                     VELEVIECNRPQTHSRAELVGLPPVDRAELERELTTALDEIKRLEAESRQGIQSSITA
                     ADRADQLRTALAETHHQLDETSRELAAAQRTLQRQNDELETLRSELSVGKQSAAEQLD
                     LQSRTHLETAALRDELTRCQADLNEARSALGGAQDERARAAEAAADVERRLADEQERW
                     RNEQKQWQEEQVQLQRRLAQRDAELEALREQSATQTGVMTVSTGHMTVRTGNVTEPAG
                     NVTEPAGNVTVPTGNITVPTGNMTVNMEEVRRETEVTENQERSKRAEAERQLSETRRE
                     LADAMARIQEAEGASARCVELESQLRDVRERAESSDERRRALDLRAAELAAQQETLQA
                     AERELAVERERLAHSIREAAQRLREQEAHAEQRATELERSLESASARLSELDSLRTQL
                     VAERDALGERAERLVQRELEAETRLASPPPLPPAQPPSEFGRLIELQDELGASQADRR
                     QLAEQIEALEVRCRELEEQRSSALAQAAAAYGNSVAEKPRRAAEPTAAERPPIVTVSF
                     QSLQAERALQEETAPAVSEAAGEPVAPANPSVALAAAEPESQQVSPPVSLFGYMEQLA
                     AKARGEKSAAEPPQIQFPLQRAAPNLMLPPVEETPAAESRPDPATAEPEASETAAQDE
                     PEAPIQTLDPEQRQQLTRWVIARLGSGAAAVAISGVLGYWAWTSNSITAGTAAAVGAA
                     IGAFWTIASIRRLGAIMALSPPEPLPQSLLDAEEAAQAKAAAEEARRAAAKKK"
     gene            5300..5746
                     /locus_tag="VFB80_06450"
     CDS             5300..5746
                     /locus_tag="VFB80_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZN33440.1"
                     /translation="MARKDALMSLRQILVRRRDALRKALAGDLSLLKELRQQNSGDVI
                     DAALDTAQDEISSQLAEVESRELTHIETALERMRKGDYGLCETCGEGIPLARLQALPY
                     ATMCIGCQREAEKDGGHGGAANWGRILDSGVADSEVSAGDLEIDVS"
     gene            5802..6896
                     /locus_tag="VFB80_06455"
     CDS             5802..6896
                     /locus_tag="VFB80_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN33441.1"
                     /translation="MQASRRFLWICAALWCAASASGRLAADDLSAGAAAARDKLFAEL
                     AADVEELERRGNVLKRVVKLVRPSIVHIEAKRDADGSRLARGEAEEAGSGVIIESHGK
                     PYVLTNRHVIRYSTLAGIHVRLADSRILTPTRVWADPATDVALLSIAADDLVPARVGD
                     SSKMEMGDFVLAVGSPFGLSHSVSYGIISAKGRRDLQLGDDGLQYQDFLQTDAAINPG
                     NSGGPLLNLRGELIGINTAIASSSGANEGVGFAIPINMVMTIAGQLVEHGVVQRSYLG
                     VRLDASFAQPQGARVSGITRNSPAELARILIGDVIVEFDGTRIEDDMHLINLVSLTPV
                     NKEVEVRLLRNGRPVQVRLRVANRSQMPAE"
BASE COUNT         1128 a         2390 c         2231 g         1226 t
ORIGIN      
        1 gacgtgcgtc ccttcgggcg tgaccgagat gatcgtgatg ccgccgccac cgccgccccg
       61 ctcgccgccg ccgccgcgcg acttcacctc gcatttgagc tcgacggtcg ccgcgttctc
      121 gacggatccc agctctgtca cgatgaattc gtagggccgc cgttcgacgg tagtcgtgat
      181 cagcttcggg cccagctcgg cactttttcg gccgtaaaac caccacccgc caaacgccaa
      241 gcccgcggca gccaccaatg ccgcaacggt cagcgtccgc acgaaacctc ggcggcgagg
      301 aacttcggcc gacatagtgc ggctcctggg agaggaaaac cggcggggca gagggcgcgc
      361 gggtgggctg caagcaggcg ggcgggcagc cgccgattcg cccggctgcc caactcttat
      421 ttgtacctca tcggcagggc gatgtcacgt gcttttgggt cagtggtcga tgggaggggg
      481 aagctcctgc tgagccgctg ctcggcggcc gcctctccca cccggacgca atctcgcggc
      541 gaccagagtg gcaagcggcg gctgcgaagg ttagattgag gcggtcgcct tggggagtca
      601 aactggcgca gcttccgccc tcacaatcat cccgccttgg agttccccag atgggcccct
      661 ctccttctgc ccgccgcgat cggcgcgggt tcacgctcgt cgagctgctc gtcgtcatcg
      721 cgatcatcgg cgtgctcgtc gcgctgctct tgcccgccgt gcaagccgcc cgcgaagcct
      781 cgcgccgcag caagtgccag aaccatctca agcagtgggc tctggcgatg cacaacttcc
      841 acgacgttta cggcaacttc ccgtacttca cgcaagtgac acccaggcgg cacaactggc
      901 cgcccttcgt catgccttac ctggagcaag gcaacctcgt gcagggttat gacttctcca
      961 tcaactggta cgatggaaag aacttgccca tgacgcaggt ccacctgacg gtgttcaact
     1021 gccccagcga ccggccgaag ccgacgtgga ccgaccaaac caattatccc tcggtgcggg
     1081 cgaactatct cgtttgctac ggcaatcatg tgtttggcag cgccacagcc gtcggcctgg
     1141 gccgcggcgt ctttggcatg acaacgccac cgactccagc cggcagcaat aacttcgtgc
     1201 cgtacaaggg ccgcttcgcc gacattaccg acggcacttc caacacgctg ctgatgtcgg
     1261 aggtgatcgt ggcccgctct gacaataacc aagggggcgg cggcaattgg cccagcggcg
     1321 attttcgcgg ccacgtctgg cacgatgcga cgtcgtccaa tcctacgcac tgtcccaaca
     1381 tcttcatgac catcaacggt cctaacagca ccgtccccga caacgccatg tgcggcacga
     1441 tcccgaacaa cgaccgggcc atgccctgcg tcaatggcgc caacaccgac cgccagaacg
     1501 tcgcccgcag ccggcatccg tccggcgtca actcctgccg ggcggacggc tcggtccaat
     1561 tcgtgagcaa cacgatcgcc ctcgcctcgt ggcgggctct cggctcgatg gatgccggcg
     1621 aagcctcgaa cgactgacac cgcctgacgg tgtcgggaac ttcttcaaac cactatccca
     1681 atcgcgttca gagtatgtct tattgtctgt gttttgtgcg gcggtgcgcc gcgctcttcg
     1741 tattgagttt gatttgcctc cccggctgta gtccaggcaa gagcacggtc agcggcacgg
     1801 tcacgctcga cggccagccc gttcaaagcg gcgcgatcac gttcgtgaaa gacgaaggcg
     1861 gctcgatggt acgcgagggc gcggtgatct ccgatggcaa gttcgaggcc gccgtgccgc
     1921 cgggaaaata caagctggag ttgaccggcc agaaggtcgt cggcaagcgg actcaaacgg
     1981 gtttcgacgg caagccagaa acgctcgacg tgaccgagca actctttccc gaaaagttca
     2041 acacgaagac cgaactgatg caggaaatca agccgggtgc aaaccccatc aatctcgact
     2101 tgaagagcta gccgaagctg tccgatgtgg caaggcggcg gctatttctt cttcgcggct
     2161 gcacgcctgg cttcttcggc cgctgccttg gcttgcgccg cctcttcggc gtcgagcagc
     2221 gactgcggca gcggttcggg cgggctcagc gccatgatcg ccccaagccg gcgtatcgag
     2281 gcgatagtcc agaacgcacc gatggcagcg ccaacggctg cggctgtgcc tgccgtaatg
     2341 gaattcgacg tccaggccca atagccgagc acgccgctga tcgccacggc ggccgccccg
     2401 ctacccaggc gggcgatgac ccagcgggtg agttgctggc gctgttccgg atcgagcgtc
     2461 tggatcggcg cttcaggttc atcctgcgct gcggtctcgg acgcctccgg ctcggcggtg
     2521 gcgggatccg ggcgcgattc cgcagcgggc gtctcttcta ccggcggcag catcagattg
     2581 ggagcggccc gctgcaacgg gaactgtatc tgcggcggct ctgcagccga cttctcgccg
     2641 cgggccttcg ccgctagctg ctccatgtag ccgaacagag agacgggtgg gctgacttgc
     2701 tggctctctg gttcagcggc ggccagagcg actgacggat tggcgggtgc gaccggttcg
     2761 cccgccgctt cggagacagc cggcgcggtc tcctcctgca gagcgcgctc ggcctgcagc
     2821 gactgaaagg atacggtcac gatcggcggc cgttcggccg cagtgggctc cgccgcacgc
     2881 ctgggcttct ctgcgaccga attgccgtaa gccgctgcgg cctgagccaa ggcggagctc
     2941 cgctgctcct cgagctcgcg gcagcggact tccagggcct cgatctgctc ggccagttgc
     3001 cggcggtcgg cctggctggc acccaattcg tcctgcagct cgatcaggcg tccgaactcg
     3061 ctcggcggct gcgccggcgg cagcggcggc ggcgacgcca ggcgggtctc ggcctccagc
     3121 tcgcgctgca cgagtcgctc ggcccgttcg cccaacgcgt cgcgttcggc gacgagctgg
     3181 gtccgcagcg agtccaattc gctcaggcgg gcgctggcgg attccaggct ccgttccagc
     3241 tcggttgccc gctgctcggc gtgcgcctcc tgctcgcgga gtcgctgcgc ggcttcgcga
     3301 atcgagtgcg ccagccgctc gcgctcgacg gccaattccc gttcggcggc ttgcagggtt
     3361 tcctgctgcg cggccaattc ggcggcccgc aggtcgagcg cccgccgccg ctcgtcgctc
     3421 gattcggcgc gctcccgtac gtcgcgcagc tggctctcca gctcgacgca gcgggcactg
     3481 gcgccctctg cctcctggat gcgggccatc gcatcggcca gctcgcgccg cgtttcggac
     3541 aattgccgct cggcctccgc ccgcttcgac cgctcctggt tttcggtaac ttcggtttcg
     3601 cggcgaacct cctccatgtt gaccgtcatg ttgccggtcg gaacggtgat gttcccggtt
     3661 ggaacggtca cattgccagc cggctcagtc acattgccag ccggctcagt cacgttccca
     3721 gtccgcacgg tcatatgccc agtcgaaacc gtcatgacgc cggtctgagt cgccgactgc
     3781 tcgcgcagcg cctcgagctc ggcgtcgcgc tgtgccagcc gccgctgcaa ttgaacctgc
     3841 tcctcttgcc attgcttctg ctcgttccgc catcgctcct gctcgtcggc cagacgtcgc
     3901 tcgacgtcgg cggctgcctc ggcggcccgc gcccgctcgt cctgcgcgcc gcccaatgca
     3961 gatcgcgcct cgttcaggtc agcctggcag cgcgtcagct catcgcgaag cgcggccgtt
     4021 tcgagatgcg tgcggctctg caagtcgagc tgttctgcgg ccgactgttt gcccacagac
     4081 agctcgctgc ggagcgtctc cagttcgtcg ttctgccgct gcagggtccg ctgtgccgcc
     4141 gccagttcgc ggctggtctc gtccagctgg tgatgcgttt cggccagcgc cgtccgtaat
     4201 tgatcggccc ggtcggcggc ggtgatcgac gactgaatgc cctgccgcga ttcggcttcc
     4261 aggcgtttga tctcgtccag ggccgtggtc agctcgcgct ccagctcggc ccgatccacg
     4321 gggggcagcc cgaccagctc cgcccgcgaa tgggtctgcg gccggttgca ctcgatcact
     4381 tccagctcca cctcgcccac gcggaaacgg tcgcccggcg acagccgcga ctcttccgcc
     4441 gccgcaccgt tcaccgacac ccggccgcgc agttgccgca ccaccgtgcc gtactttccc
     4501 cgcagcaccc agcagtgcag cggagcgact cccgcgccca tcagccgcag cgtgcagccg
     4561 ctcgccgaac cgatcgtgca cttgggcgag cgaatgcgga gcacccgtcc gtcgtgttcc
     4621 tggccgcgga ccttgagcgc cagctcgccg cagtcggcga gaaacctgct ggggcgtggc
     4681 ggtgaacatg cgagcatcga atcaccttgc ggcgggactc tgggcgacga tcggggcgca
     4741 aacccccagg gcatctagct tgcggcccgt cccccgtcaa aacagtttgc tgacgccgag
     4801 gcggtggcag attttgccgc actgccgcca aatcggctgc atcgcctgcc cgtttttgcc
     4861 gattatcgga aggcggcaaa cgctgccgga tatgccgaac ttgcggacgc ctttcggctt
     4921 gccgcaagtt tgaagtgtcc ccttcggcca tggccacgat ttatggtcgc catcgagcct
     4981 gcggggcgac gcctccgacg cgttataacc tattgacaac aaagcggttg cagctcgcaa
     5041 gccggcatac gaccgagctg caactgggtt cagccacagc gggagagggt agccggcccc
     5101 tgtagtatat tttgattgtt ttgcgccgaa atggcgtgcg aggactggac aatccggtct
     5161 ggcatgccgc tagcagccgg aacagcgcta gccattggta acgaacaacc actggtaaca
     5221 agcaacccta gaggcacgct ttgctcctgt gggcggtggg cttttggacc catagaagcg
     5281 agcgacacga ggtggatcga tggcacgcaa agacgcgctt atgagcttgc ggcagattct
     5341 cgttcgccgc cgcgacgccc tgcgcaaggc gctggcgggc gacctgagcc tgctcaagga
     5401 attgcggcag cagaactccg gcgacgtgat cgacgcggcg ctcgatacgg cgcaggacga
     5461 gatcagctcg cagctggcgg aagttgaaag ccgggagctg acgcacattg agaccgcctt
     5521 ggagcggatg cggaagggcg actacggcct gtgcgagacc tgcggcgagg gcattccctt
     5581 ggcgcggctc caggcgctgc cctatgccac catgtgcatc ggctgccagc gggaggccga
     5641 gaaggacggc ggccacggcg gcgcggcgaa ttggggacgc atcctcgact ccggcgttgc
     5701 cgacagcgaa gtttcggcgg gcgatctgga aatcgacgtc tcgtagccgc tcgccagact
     5761 gattccggcc cggcgcacct tggaaaggat gccagaagcg catgcaggcc tctcgtcgct
     5821 tcctctggat ctgcgccgcg ctgtggtgcg cggccagcgc gtccggccgg ctcgcggctg
     5881 atgacctgtc ggctggcgct gcggctgccc gcgacaagct cttcgccgaa ttggccgcgg
     5941 acgtcgaaga attggagcgc cggggcaacg tcctcaagcg cgtggttaag ctcgtccggc
     6001 cgtcgatcgt gcacatcgag gccaagcgcg acgccgacgg cagccgcctg gcccgcggcg
     6061 aggccgaaga ggccggctcg ggtgtaatca tcgagagtca tggcaagcct tacgtattga
     6121 ccaaccggca cgtgatccgc tattcgacgc tggcgggaat tcacgtccgc ctggccgaca
     6181 gccgcatcct caccccgact cgggtctggg ccgatccggc gaccgacgtg gcgctcttgt
     6241 cgatcgcggc cgacgatctg gttccggccc gcgtgggcga ttcgagcaaa atggaaatgg
     6301 gcgacttcgt gctggcagtc ggcagcccgt tcggcctgag ccattcggtg tcgtacggca
     6361 tcatcagcgc caagggccgc cgcgatttgc agctggggga cgacggcctt cagtaccagg
     6421 acttcctcca gactgacgcc gcgatcaatc ccggcaacag cggcgggccg ctgctcaacc
     6481 tgcggggcga gctgatcggc atcaacacgg ccatcgccag ctccagcggc gccaacgaag
     6541 gcgtcggctt tgccatcccg atcaacatgg tgatgaccat cgccggccag ctcgtcgagc
     6601 acggcgtcgt gcagcggtcc tatctgggcg tgcggctgga cgcgagcttt gcccagccgc
     6661 aaggcgcgcg ggtcagcggc atcacccgca actcgcccgc cgagctggcc cgcatcctga
     6721 tcggcgacgt cattgtggaa ttcgacggca cgcggatcga ggacgacatg cacctgatca
     6781 acctggttag cctcacgccc gtcaacaagg aagtcgaagt ccgcctgctc cgcaacggac
     6841 gcccggtgca agtccgcctt cgcgtcgcca accggtctca aatgccggcg gaatgagcct
     6901 ggtgcagacc gctcgctccg cgagcggact tcttcacgcg gagcgtgagg actaccgacg
     6961 gtcgcccggc ggatc
//
LOCUS       DASRJB010000234         5497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_917376, whole genome shotgun sequence.
ACCESSION   DASRJB010000234 DASRJB010000000
VERSION     DASRJB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917376"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFB80_06460"
     CDS             complement(<1..349)
                     /locus_tag="VFB80_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33442.1"
                     /translation="MNRKMLGLAAVLLLVIGVIVAVQQPEGGAAFAGACVRVGLVLLA
                     IWLALPQVTRFWNQTPKWLLVAAAIALVVCVIHPLYALVAIPLMGLLWFLGPKVMSLW
                     KPKAPGGPPAKSAS"
     gene            complement(370..1668)
                     /locus_tag="VFB80_06465"
     CDS             complement(370..1668)
                     /locus_tag="VFB80_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33443.1"
                     /translation="MLARRLVLVSLCAFAALTLAGRSRADNWPQWRGVRGDGVCDERN
                     VPIRWSKTENVLWRLPLPGPAGATPVVWGERIFLTSVAGPEKSDLVLLCVGAGGQPLW
                     QETIARGSKDVRNDEGNYASPSPSTDGKHVWAFFGTGDLACFTVEGKPVWHCNVQERF
                     GKFNIQFGMASTPVLDRGRLYLQLIHGDGNPATREARVVCLDAASGDTIWQTERPSDG
                     RQECEHSYASPVLYRDAARSFLVTHGADYVVAHDLADGRELWRCGDLNPKGQYNNTLR
                     FVASPVAVPGLIVVPSAKKLGMLGLMPDGSGDLTDNLAATYWRWTNNTTDVPSPLVAG
                     DLLYVCKEGGELLVAEAKSGEIVYLEPTTRDRHRASPVFADGHVYLSARNGTITVVKA
                     GRKFEIVAKNELGESTSASPIFANGRLYVRTFDALYAIGK"
     gene            complement(1778..2266)
                     /locus_tag="VFB80_06470"
     CDS             complement(1778..2266)
                     /locus_tag="VFB80_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZN33444.1"
                     /translation="MDALLLIDGYNLLHSAGVFVESGPPTLERSRAALLDALLERLPA
                     KLAARTIVAFDAEGAPPGLPREISHRGLAVRFARRGRSADELLEELIAAAADPRNVTV
                     VSSDHRVQRAARQRGTAFIDSERWWHELRQHSAAADPTDEKSAAPPGNPFPPGYARDL
                     FR"
     gene            complement(2290..3369)
                     /locus_tag="VFB80_06475"
     CDS             complement(2290..3369)
                     /locus_tag="VFB80_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33445.1"
                     /translation="MKLIRTILTLAAVAAVATPLAAADARIAIIDVGGGQTMAARCDA
                     AGTIHVVTQTPGGPQYARSTDGGESFSQPLPVVSAKARQPGLEYTVWDVAVTKEGGVH
                     VALGTNAWKLKLPKEEWAFFYARLEPGAQAFAPVVNINRKPSEGFSLAADDSGNVTAC
                     WMSGKLYANVSHDGGRTFGPTVEIDPSFDPCDCCTTSATYAADGRLALLYREETNNER
                     NMFLVQWDQKQNVATRTRVSTTPWNIDACPMTYYSIVPQGDGFVAAWPTKGEVYWARL
                     DGQGTLQSPGEIKTPGQSGMRSGVLTLTDTSGNTLVAWKKAEQLGWQLYDAQGRASGK
                     TASAKSRGSGAAGVVSKDGRFLLFP"
     gene            3555..4979
                     /locus_tag="VFB80_06480"
     CDS             3555..4979
                     /locus_tag="VFB80_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HZN33446.1"
                     /translation="MCLPYRPCQWAIACWCVLSVSLLVPRPAAGQGGAVAFTQQRPFV
                     IGFIPVVGPNGGVGGVSIDAQGVVSRSNVDQLGLLRDARLKAQLPPSREMPQASTLRK
                     VSLKGLSAELDRLRKTGKPITDDLQNLAGLTRVEYVLVYPDQRDIVLAGPAEAWRIDE
                     QATAVGKTSGQPVLQLDDLLVALRAAMQQRASGGAITCSIEPTKEGLTRYTRALASHS
                     GQPDEATVARLEAAMGPQQVLISGVAPGTHFARVLVAADFVMKRLGMNFEPAPVEGLP
                     SYLEMLQDPAVPAPKNAMPRWWMAAHYEPLLKDEDGLAWQLQGAGVQTLAEDGVQGNK
                     GRVIAVRHSDGSLSKKWADLMTAKYAGLAAKLPIFAELRNCMDLAVVAALLVKEDLPA
                     RSGCDLGLLLDGKQLPVAEQHAPRTIDSRASLVRKGRQTIISVSGGVEVDPWAVLERT
                     QVESALAATRTTSASAKPDRWWWD"
     gene            complement(5048..>5497)
                     /locus_tag="VFB80_06485"
     CDS             complement(5048..>5497)
                     /locus_tag="VFB80_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN33447.1"
                     /translation="LAQPADYEGLMKLVIDSFEPITWQKKLDARIGPLNGRDWRQRWQ
                     ARLRNIFATQIVLVGQCDGQLAAMASGTIDREAALGFIDVLCVGREFQGQGLGREMLR
                     AMLDHFRAEGSQYVNLDCLTDNETANALYQSEGFEEVARHIRWFRKI"
BASE COUNT          901 a         1861 c         1825 g          910 t
ORIGIN      
        1 gcgaagccga tttcgcgggc gggccgccgg gtgccttggg cttccacagc gacatcacct
       61 tcggccccag gaaccagagc aaccccatta gcggaatcgc caccagcgcg tacagcggat
      121 gaatcacgca cacgacaagt gcgatagcag ccgccaccag cagccacttg ggcgtctggt
      181 tccagaagcg cgtcacctgc ggcagcgcca gccagatggc caacagcacc aggcccacgc
      241 ggacgcacgc cccggcaaat gccgcgccgc cttccggctg ctgcaccgcg acgatgacgc
      301 cgatgacgag cagcagaacg gcggccagac ccagcatctt gcggttcatg cgtccattat
      361 acgccgcgat cacttgccga tcgcatacag ggcgtcgaac gtgcggacgt acagccggcc
      421 gttggcaaag atgggtgagg cggaagtcga ttcgcccagc tcgtttttgg ccacgatctc
      481 gaacttgcgg ccggctttga cgaccgtgat cgtgccgttg cgggcgctga gatagacgtg
      541 gccgtcggca aagaccggcg aggcccggtg gcggtcgcgg gtcgtcggct ccagataaac
      601 gatctcgccg ctcttggcct ccgctacaag caattcgccg ccttccttgc agacatagag
      661 caagtcgccg gcgaccagcg gcgagggcac gtcggtcgtg ttgttcgtcc accgccaata
      721 ggtcgccgcc aggttgtcgg tcaagtcgcc gctgccgtcg ggcatgaggc cgagcatacc
      781 cagcttctta gctgacggga ccacgatcag ccccggcacg gcgaccggcg aggccacgaa
      841 ccgcagcgtg ttgttgtatt gccccttggg gttgaggtcg ccgcagcgcc acagctcgcg
      901 gccgtcggcc aggtcgtggg cgacgacgta atcggccccg tgcgtcacca gaaacgaccg
      961 cgcggcgtcg cggtagagca ccggcgaggc gtagctgtgc tcgcactctt gccggccgtc
     1021 gctgggccgc tcggtctgcc agatcgtgtc gccgctggcg gcgtcgaggc agaccacgcg
     1081 ggcctcgcgc gtcgccggat ttccatcacc gtgaatcagc tgcaaataca gccggccacg
     1141 gtcgagcacg ggcgtgctgg ccatgccgaa ctggatgttg aacttgccaa accgctcctg
     1201 gacgttgcag tgccagaccg gcttgccttc cacggtgaag cacgccaggt cgcccgtgcc
     1261 gaagaaggcc cacacgtgct tgccgtcggt cgatggagag ggcgaagcgt agttgccctc
     1321 atcgttgcgg acgtccttgc tgccgcgggc aatggtctct tgccagagcg gctggccgcc
     1381 cgcgccgacg caaagcagca ccaggtccga cttctccggg ccggcgacgc tggtcaggaa
     1441 aatccgctcg ccccacacga cgggcgtcgc cccggccggc cccggcagcg gcaagcgcca
     1501 cagcacgttt tcggttttac tccaacggat cgggacgttt ctttcgtcgc acactccatc
     1561 gccgcggacg ccccgccact gcggccagtt gtcggcccgc gagcggcccg ccagagtcag
     1621 ggcggcgaag gcgcagagcg aaaccagaac caggcggcga gcaagcatat tgctgttcct
     1681 caaggacatt ccaggcgggc gaaagcaggc gggatcgttg gcgggctcgt tccagtttgg
     1741 caaccgcgcc ctggatggtc aagttcgacg tgccggctta ccggaacaag tcgcgggcat
     1801 acccgggcgg aaacgggttg cctggcggcg cggcggactt ctcgtcagtc ggatcggctg
     1861 cggcggagtg ctggcgcagc tcgtgccacc agcgctcgct gtcgatgaac gccgtccccc
     1921 gctgccgtgc ggcccgctgc acgcggtggt cgctcgacac gaccgtcacg ttccgcgggt
     1981 cggcggctgc cgcaatcagt tcctccagca attcatcggc cgagcggccg cggcgggcaa
     2041 accgcacggc cagcccgcga tgcgagattt cccgcggcaa tccgggcggc gctccctcgg
     2101 catcgaacgc gacgatcgtc cgcgccgcca gcttcgccgg aaggcgctcc agtagcgcat
     2161 ccaagagcgc cgcccgcgaa cgctccagcg tcggcgggcc ggactcaacg aagacgccgg
     2221 cgctatgcag caggttgtag ccgtcgatca acaggagcgc atccatctgc ctggtcccga
     2281 tcaactcggc tacggaaaca gcagaaatcg cccatccttg ctgaccacgc ccgccgcgcc
     2341 gctgccacgg cttttggcgg aagcggtttt acccgacgcg cggccctggg cgtcgtacag
     2401 ctgccagccg agctgctcgg ccttcttcca ggcgacaagc gtgttgccac tggtgtcggt
     2461 gagtgtcagc acgccgcttc gcatgccgga ctgtccgggc gttttgatct cgcccggcga
     2521 ctgcagcgtg ccttggccat cgagccgggc ccagtagact tcgcccttgg tcggccaggc
     2581 ggcgacgaag ccgtccccct gcggcacgat cgagtaatag gtcatcgggc aggcatcgat
     2641 attccagggc gtcgtgctga cgcgggttcg cgtcgccacg ttctgcttct ggtcccactg
     2701 cacgaggaac atgttccgct cgttgtttgt ctcttcgcga tagagcaggg ccaggcggcc
     2761 gtcggcggcg taggtcgcgc tcgtcgtgca gcagtcgcag gggtcaaacg acggatctat
     2821 ctcgacggtg gggccgaacg tccggccgcc gtcgtgcgag acattggcgt agagcttgcc
     2881 cgacatccag caggcggtca cgttgcccga atcgtccgcc gccagcgaaa atccctcgct
     2941 cggcttgcga ttgatgttca ccaccggcgc gaacgcctgg gcccccggtt ccagccgcgc
     3001 gtagaagaag gcccactcct ccttcggcag cttcagcttc caggcgttcg tgcccaaggc
     3061 cacgtgaacg ccgccctcct tggtcacggc cacgtcccaa accgtatatt ccagccctgg
     3121 ctgccgcgct ttggcactga ccacgggcag cggctggctg aagctctcgc cgccgtcggt
     3181 ggaacgagcg tactgcggcc cgccgggcgt ctgggtgacg acgtggatcg tgcccgcggc
     3241 atcgcatctg gcggccatcg tctgcccgcc gcccacatcg ataattgcaa tgcgggcgtc
     3301 agctgctgcc agcggagtcg ccacggccgc aaccgcagcg agcgtcagga tggtgcgaat
     3361 caacttcatc gaatggctcc cttcgcacgt atcgtaccgt gccacgttgc aaccgggaat
     3421 tcattcgcga taatgaggac cgagctgaac gttcgccgcc acccgcatct tgttggcctc
     3481 cttgagactc cgcggcgacc ggccagttga ttgacgccag gggcaatggc tcgatctgga
     3541 acgaggaggc cgttatgtgt ctgccgtatc gcccctgcca gtgggctatc gcctgctggt
     3601 gcgtcctgag tgtatctctg ctggttccgc ggcccgctgc cggccagggg ggcgcggtgg
     3661 cgttcaccca gcagcggccg ttcgtcatcg gcttcattcc ggttgtcggg cccaatggcg
     3721 gcgtcggcgg cgtctcgatc gacgcgcagg gcgtcgtctc gcgcagtaat gtggaccagc
     3781 tggggctgct gcgcgacgca cggctaaaag cccagttgcc gccgagccgc gaaatgccgc
     3841 aggcgagcac gctgcgcaaa gtttcgctca aagggctctc ggccgagctg gaccggctgc
     3901 ggaaaaccgg gaagcccatc accgacgatt tgcagaatct ggccgggctg acgcgcgtcg
     3961 aatacgtgct ggtctatccg gaccagcgcg acatcgtcct ggccggtccg gcggaagcct
     4021 ggcggatcga cgagcaggct accgcagtgg gcaaaacgag cggacagccg gtgctccagc
     4081 tcgacgacct gctcgtcgca ctgcgggcgg cgatgcagca gcgggccagc ggcggtgcga
     4141 ttacctgctc gatcgagccg acgaaagagg gcctgacgcg ttacacccgc gccctggcga
     4201 gccattccgg ccagcccgac gaggcgaccg tcgcccggct ggaagcggcg atggggccgc
     4261 agcaggtgct catcagcggc gtcgcccccg gcacgcattt cgcccgcgtg ctggtggcgg
     4321 ctgattttgt gatgaagcgc ctgggcatga atttcgagcc cgcgccggtc gaggggctgc
     4381 cgagctatct cgaaatgctc caggatccgg ccgtgcccgc gccgaagaac gccatgccgc
     4441 ggtggtggat ggcggcgcac tacgagccgc tgctcaagga cgaagacgga ctcgcctggc
     4501 aactgcaggg agcgggcgtg cagacgctcg cggaggacgg cgttcaggga aacaagggcc
     4561 gcgtgatcgc ggtccggcat agcgacggct cgctctcgaa aaaatgggcc gacctgatga
     4621 cggccaagta cgcgggcctc gcggcgaagc tgccgatctt tgccgagctc cgcaactgca
     4681 tggacctggc cgtcgtcgcg gcgctgctgg tcaaggaaga cctgccggcc cgctccggct
     4741 gcgacctggg gctattgctc gatgggaagc agctgccggt cgccgagcag cacgcgccgc
     4801 ggacgatcga ttcgcgggcc agcctcgttc gcaaaggccg gcagacgatc atcagcgtct
     4861 cgggcggcgt cgaagtcgat ccctgggcag tcctggagcg aactcaagtc gagtcggccc
     4921 tggccgcaac ccgaacgacc tcggcgtcgg ccaaacccga ccgctggtgg tgggactgac
     4981 ctctcaaagc cgcgaccagt ggtcgcggtg cattaggtag accgcgacca ctggtcgcgg
     5041 ctttgcgtca gatcttccgg aaccaccgga tgtgccgggc gacttcttcg aagccttcgc
     5101 tctggtagag ggcgttggct gtttcgttgt cggtcaggca gtcgaggttc acgtactggc
     5161 tgccttccgc ccggaagtgg tccagcatcg cccgcagcat ctcgcggccc aggccttggc
     5221 cctggaattc tcggcctaca cagagcacgt cgatgaaccc cagcgccgct tctcgatcga
     5281 tcgtgccgct ggccatcgcc gccagctgcc cgtcgcactg gccgaccagc acgatctggg
     5341 tcgcgaagat gttccgcaag cgagcctgcc accgctggcg ccaatcgcgg ccgttgagcg
     5401 ggccgatgcg ggcgtcgagc ttcttctgcc acgtgatggg ctcgaagctg tcgatcacca
     5461 gcttcatgag cccctcgtaa tcggcgggct gggccag
//
LOCUS       DASRJB010000235         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_350026, whole genome shotgun sequence.
ACCESSION   DASRJB010000235 DASRJB010000000
VERSION     DASRJB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350026"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..2019)
                     /gene="nadE"
                     /locus_tag="VFB80_06490"
     CDS             complement(31..2019)
                     /gene="nadE"
                     /locus_tag="VFB80_06490"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZN33448.1"
                     /translation="MKLLRVGAAVLNQTPLDWDGNRARISGAIESARAAGVSLLCLPE
                     LCITGYGCEDAFHAPGTQQIALEVLRELLPQTRGMVVSLGLPLLYHGSLFNTACIAVN
                     GKLLGFVGKQNLAGEGIHYEPRWFKPWPKDVQVEVPIGEGLYPLGDLVFDCGGVRLGF
                     EICEDAWVADRPGGQLARKGVDLILNPSASHFAFGKHEIRKRFVTEGSRAFNTGYLYA
                     NLLGNESGRAIYDGDAMIAAAGKLVVTGPRFSYADWHVTHAVLDIDAIRMSRARTGSF
                     RPTIRSDTSDTIGCSFDFPELKPEKAAFSQAAWESSPHIKEEEFTRAVSLALFDYVRK
                     SRSQGFVVSLSGGADSAACSYLSAKLVDFALADIGRERLLQKLRHIKSLEQAADAKAI
                     VKRLLACVYQATRNSGDVTRTAARAVAQSLGAEYFEFDVDAVVQNYIGLVSRGIGREL
                     DWKTDDVALQNIQARGRAPSVWLLANLRSALLLATSNRSEAAVGYATMDGDTSGGLSP
                     IAGIDKAFIRRWLQWVEKLGPDGIGPLAELAVVNEQAPTAELRPPSAKQTDEDDLMPY
                     ELLDAIERAAIRDKQSPVEILQLMAAEFPQYSRPQLGQWIERFFRLWCRNQWKRERYA
                     PSFHLDDENLDPKTWCRFPILSGGYERELAELRKLAAN"
     gene            complement(2046..>2429)
                     /locus_tag="VFB80_06495"
     CDS             complement(2046..>2429)
                     /locus_tag="VFB80_06495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN33449.1"
                     /translation="RLADISAKGLTFARQRASEASVKIQTLETDLEEDPFPAGPWDVI
                     LSVCYLRRSLYAVFPTVLAPGGTLAVVQPTMTNLTRHPKPPADYLLNDGELPSLVRGL
                     EVVYYEEGWLADDRHDAVLVAKVPI"
BASE COUNT          418 a          786 c          804 g          421 t
ORIGIN      
        1 cgcctttggc tacgggttaa acgaggaggc ctaattggcc gccagtttcc gcaactcggc
       61 cagttcgcgc tcgtagccgc ccgatagaat cgggaaccgg caccaggtct tgggatccag
      121 attctcgtcg tcgaggtgga agctgggggc atagcgctcg cgcttccact ggttgcggca
      181 ccacagtcgg aagaaccgct cgatccactg gcccagctgc ggccgactgt actgcgggaa
      241 ttcggccgcc atcagttgca agatttcgac cggcgattgc ttgtcgcgaa tggctgcccg
      301 ctcaatggcg tccagcagct catagggcat caagtcgtct tcgtcggttt gtttggccga
      361 cggcggccgc aactcggccg tcggcgcttg ttcgttgaca actgccaact cggccagcgg
      421 gccgatccca tccggcccga gcttctccac ccactgtagc cagcggcgga taaaggcctt
      481 gtcgatgccg gcgatcggcg agaggccgcc gctggtgtcg ccgtccatcg tggcatagcc
      541 cacggcggcc tccgagcggt tgctcgtggc cagcagcaga gcgctgcgca gattcgccag
      601 cagccagacg ctcggggctc ggccgcgggc ctggatgttt tgcagcgcca cgtcgtcggt
      661 cttccaatcc agttcgcggc cgatgccgcg cgagacgagg ccgatgtagt tctgcacgac
      721 ggcgtccacg tcgaactcaa agtattccgc gccgagcgac tgggcgaccg cgcgggccgc
      781 ggtgcgggtc acgtcgccgc tgttgcgcgt cgcctgatag acgcaggcca ggagcctctt
      841 gacgatcgcc ttggcatcgg cggcttgctc cagcgacttg atgtgtcgca gcttctgcag
      901 caggcgctcg cggccgatgt cggccagggc gaaatcgacg agcttcgccg agaggtacga
      961 gcaggcggcg gaatcggccc cgcccgacag cgacacgacg aaaccctgcg agcggctctt
     1021 gcggacgtaa tcgaacaggg ccagcgacac ggcccgcgtg aactcttctt ccttgatgtg
     1081 cggcgacgat tcccaggcgg cctgcgagaa cgcagccttc tcgggcttga gctccggaaa
     1141 atcgaacgag cagccgattg tgtcggaggt gtcgctgcga atcgtcgggc ggaagctgcc
     1201 ggtgcgggcg cgggacatgc ggatcgcgtc gatgtcgagc acggcgtgcg tcacgtgcca
     1261 gtcggcatag gagaaccgcg gcccggtgac gaccagcttg ccggccgcgg cgatcatcgc
     1321 gtcgccgtcg tagatcgccc gccccgattc gttccccagg agattcgcgt agagataacc
     1381 cgtgttgaac gcccgcgagc cctcggtgac gaaccgcttg cggatttcgt gcttgccgaa
     1441 ggcaaagtgg ctggcgctgg ggttgaggat cagatcgacg cctttccgag ctagctgccc
     1501 gcccgggcgg tccgcgaccc aggcgtcctc gcagatttcg aatcccaatc gcacgccgcc
     1561 gcagtcgaac accaggtcgc ccagcgggta aagtccctct ccgatgggca cttccacctg
     1621 cacgtccttc ggccagggct tgaaccatcg cggctcgtag tgaatgcctt cgcccgccag
     1681 gttctgcttg ccgacgaagc ccaagagctt gccattgacc gcgatgcagg cggtgttgaa
     1741 cagcgagccg tgatagagca gcggcaggcc gagcgagacg accataccgc gagtctgcgg
     1801 caacagctcg cggagcacct ccagggcgat ctgctgcgtg ccgggagcgt ggaaggcatc
     1861 ctcgcagccg tagccggtga tgcacagctc gggcaggcag agcagactga cgccggcagc
     1921 gcgagccgac tcgatggccc cggagatccg cgcgcggttg ccgtcccaat cgaggggcgt
     1981 ctggttgagg acggccgcgc cgacgcggag aagcttcatg gattgcagat ttcagattgc
     2041 agatttcaga ttgggacctt ggctaccagc accgcgtcgt gccggtcgtc ggcgagccag
     2101 ccttcttcat aataaacaac ctccagaccc ctcacgaggc tcggaagttc gccgtcgttc
     2161 agcaaatagt cggccggcgg cttgggatgc cgcgtcaggt tcgtcatcgt cggctgcacg
     2221 acggccaggg ttccgccggg cgccagcacc gtcggaaaaa cggcatacag agagcgccgc
     2281 agatagcaga cgctgaggat tacgtcccac ggcccggcgg gaaacgggtc ttcctccagg
     2341 tcggtttcga gcgtctggat cttcacgctc gcctccgaag cccgctggcg ggcgaatgtc
     2401 aggcccttgg ccgaaatatc ggccagcct
//
LOCUS       DASRJB010000236         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1194671, whole genome shotgun sequence.
ACCESSION   DASRJB010000236 DASRJB010000000
VERSION     DASRJB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194671"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFB80_06500"
     CDS             <1..204
                     /locus_tag="VFB80_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33450.1"
                     /translation="LVLHVAEHPDTSAQAQRLGNVLKEAGVPVRVFGGRETTHTKINA
                     DLGLPDDPATKALFEFVAEALKK"
     gene            complement(219..>2391)
                     /locus_tag="VFB80_06505"
     CDS             complement(219..>2391)
                     /locus_tag="VFB80_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33451.1"
                     /translation="DLIEETLTTGVDGRYEFRGIDPTSECRVWAGREGAFSHGVSVVK
                     GDRPALMLSISPTNAVRLSGRVLVAGGKPASGAKLEVWRQPWMPPGNEPEPERVALPE
                     ATEMSTDNEGRFTSPPLPPTAAYRVSLADPRYEPAHSAWADAASGKELPPAELLARTL
                     GEHLGLVRDAAGKPVDGALVEFRGGRSRVSARTDAAGKFSLKPAPDGPGLLFVAQRGY
                     WFHGQRIESLGAPVEVRLAGLGEAYDQPLRPAPPTTPQAERQKIAQQLFDELIKAAAT
                     DLTGDTRLRVLMKWAEVNPAAAIEFVTKKPSLIAMNNDGVRFEAANSLLHTAPDDALE
                     IIEAMGSGHMPVLMYVRLAAALPKENRQRKLELLAEANVKAQAIKEPGFRLISIAQVA
                     EALLDLGEKERGTKLLTENLETARKLNRSGYDAYLRGSFAEELSQVDLDAALALVNEI
                     TDHFEKTRHLANIAQEMAGVRPAEAEKILVNLEPPPKDSRLIDQRDHYITIASYRMAP
                     VDLPRIKRLVDSMTDPFHQAHARGVMAVALAKTNAKESRELIRSAFDALDAAAAKKPS
                     ELPVSTGNFGNIGGWLVLHAQRIDPELAAESRWRLLRLLPEEASADPQYRWRDTEAIS
                     SAAMFIAEIDAPLARELLTRLDGDLQLTISGSSRTWLPAWGLVDPAEAVRRAAGLGDE
                     RIRTNAKTTLLSAVAATGEVREQLLHYQAGLWRIDVEDIDH"
BASE COUNT          360 a          824 c          790 g          417 t
ORIGIN      
        1 ctggtcctgc acgtcgccga gcatcccgac accagtgctc aggcgcagcg tctgggcaac
       61 gtgctcaagg aggcgggcgt gcccgtcagg gtgtttggcg gccgggagac cacgcacacc
      121 aagatcaacg ccgacctggg cttgcccgac gatccggcga cgaaggccct gttcgagttc
      181 gtcgccgaag ctttgaaaaa gtgagctgcc gcgaatcctc agtgatcgat gtcctcgacg
      241 tcgatccgcc acaggccggc ctgatagtgc agcagctgct cgcggacttc gcccgtggcc
      301 gctactgccg ataacagcgt ggtcttcgcg ttggttcgaa tccgctcgtc gcccagcccg
      361 gccgcccgcc gcacggcttc ggccggatcg accaggcccc acgccggcaa ccacgtgcga
      421 ctgctgccag aaatggtgag ctgcaaatcg ccgtccagcc gcgtcagcag ctcgcgggcc
      481 agcggcgcat cgatctcggc gatgaacatc gccgccgagc tgatcgcctc ggtatcgcgc
      541 cagcgatact gtggatcggc cgaggcctcc tccggcaaca ggcgcagcag tcgccagcgg
      601 ctctcagcgg ccagctcggg gtcgatccgc tgggcgtgca gcaccagcca gccgccgatg
      661 ttaccaaagt tgccggtaga gacgggcagc tcactgggct tcttggccgc ggcggcgtcg
      721 agcgcgtcga acgccgagcg gatcagctcg cgcgactcct tggcattagt cttggccagc
      781 gcgacggcca tgaccccgcg ggcatgggcc tgatgaaacg gatcggtcat cgagtccacc
      841 agccgtttga tccgcggcag atcgaccggg gccatgcgat acgaggcgat cgtgatgtag
      901 tggtccctct gatcgatcaa tcgcgaatct ttgggcggcg gctcaagatt caccaggatt
      961 ttttcggctt cggcgggcct gacgccggcc atctcctgcg cgatgttcgc caggtgccgc
     1021 gtcttttcaa agtggtccgt gatctcgttc acgagggcca gcgccgcgtc caaatcgacc
     1081 tgcgagagct cttcggcgaa ggagccccgc agataggcgt cgtagccaga gcggttgagc
     1141 ttgcgcgcgg tctccagatt ttccgtcagc agcttcgtgc cgcgctcctt ctcacccagg
     1201 tcgagcaggg cttcggcaac ctgcgcgatc gagatcaggc ggaagcccgg ctccttgatg
     1261 gcctgggcct tgacgttcgc ctcggccagg agttcgagct ttcgctggcg gttttccttg
     1321 ggcagggcgg cggccagacg cacatacatc agcacgggca tgtggccgct gcccatcgct
     1381 tcgatgattt cgagcgcgtc gtcgggggcg gtgtgcagca gcgaattcgc cgcctcaaag
     1441 cggacgccgt cgttgttcat ggcgatcagc gagggctttt tcgtcacgaa ctcgatggcc
     1501 gcggccggat tcacctcggc ccacttcatg agcacgcgca gccgcgtgtc gcccgtcaga
     1561 tcggtggccg cggccttgat cagctcgtca aacagctgct gggcaatttt ctgccgctcg
     1621 gcctgcgggg tggtgggcgg ggccggacgc aatggctggt catacgcctc gcccagtcct
     1681 gccaggcgca cttccaccgg tgcgccgagc gactcgatcc gctgcccatg aaaccagtag
     1741 ccgcgctgcg cgacgaacag cagccccggc ccatccggcg cgggcttcag gctgaatttg
     1801 ccggccgcgt cggtgcgggc cgagacgcga cttcgcccgc cgcggaattc gacgagtgcg
     1861 ccgtccacgg gctttcccgc ggcgtcgcgc acgaggccca gatgctcgcc gagcgtgcgc
     1921 gcgagcagct ctgcgggcgg caattctttg ccgctagcgg catcggccca cgcgctgtgc
     1981 gccggctcat accgcggatc ggccagcgac acgcgatagg cggctgtcgg cggcagcggc
     2041 ggcgatgtga agcggccctc gttgtcggtg gacatctccg tggcctcggg aagagccaca
     2101 cgctccggct cgggctcgtt gcccgggggc atccagggct gccgccagac ttcgagcttt
     2161 gccccgctgg ccggcttgcc gcccgccacc agcacccgcc cgctcaggcg gaccgcattc
     2221 gtggggctga tgctgagcat cagcgccggc cggtcgccct tgacgacgct cactccgtgg
     2281 ctgaacgcgc cctcgcggcc ggcccacacc cggcactcgc tcgtcggatc gatcccgcga
     2341 aattcgtagc gtccgtcaac gcccgtggtc agcgtctctt cgatcaggtc g
//
LOCUS       DASRJB010000237         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_917912, whole genome shotgun sequence.
ACCESSION   DASRJB010000237 DASRJB010000000
VERSION     DASRJB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917912"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFB80_06510"
     CDS             complement(<1..355)
                     /locus_tag="VFB80_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN33452.1"
                     /translation="MGYSPAYSWFVLALLTLLNVVNFVDRQLVASLQVPLKADTQLQL
                     DDLKITLLAGYAFAVVYAVAGLFLGTVADRASRPRLIAAGLLVWSAMTAASGLAQSFW
                     QLAALRVFVAIGEATL"
     gene            complement(417..1442)
                     /locus_tag="VFB80_06515"
     CDS             complement(417..1442)
                     /locus_tag="VFB80_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN33453.1"
                     /translation="MTRYHWLVLGLLIGAASSATAADPITVEQPRIRAAIEKSLPLLT
                     KGAVGHRESRTCFACHSQGLPVLALTAAKSKGFAIDDEELKKQLAHTAKFLAGNRENY
                     LKGQGQGGQADTAGYALWTLAAGGCSPDETTGAVAEYLLQRHQDRDHWGNTSKRPPTE
                     ATPFTTTYVALYGLNTFGTTEQAERIAKRKQQAREWLLKTPAQDNEERVFRLWGLEAA
                     AAPAEEIAAAAKELMAKQRADGGWAQLDSGEPASAIESDAYATGTALVALHEAGGMAT
                     SDAAFQRGLAYLLKEQQDDGSWRVTTRSKPIQAYFETGFPHGKDQFISTAASSWATWA
                     LVLAYEQ"
     gene            1591..2913
                     /locus_tag="VFB80_06520"
     CDS             1591..2913
                     /locus_tag="VFB80_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN33454.1"
                     /translation="MPPDDSYAQALAAVEQTLDRVRQCSDEEKAKLRAELAGLRQMHD
                     KLTSGRVEIVIFGEISTGKSALINALVGREVASVDVRGGWTREVWHVAWDGTGYTLPG
                     LGDSQVVLVDTPGINEVGGGPRGEMAREAAQRSDLVLFVTDSDLNETEHTSLVELAAF
                     HKPLVVVLNKLDLYSPDQRDKLLAVIRNQRLRDVVPPEHVITAAADPREREYVIQAAN
                     GSERSEWRKPPPDVAELKALVLELLDREGLSLIALNAALYAADKSDRVAALRVQLRNS
                     RAQQVIWSYATLKVIGVGLNPVPIADVVGGTAIDAAMIGTLAAVYGLQMSRTHASRLA
                     ASIAQAAGLMTVSVVASTALMSLLKGLTFGKSTLLTAVPQGLAGGFGSYIVGQAAQYY
                     FEHGASWGGESPKSVAKKILSQTDKASVLTRLKGEIGKRLAINPYAKG"
     gene            3015..3314
                     /locus_tag="VFB80_06525"
     CDS             3015..3314
                     /locus_tag="VFB80_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33455.1"
                     /translation="MTVTSLDQPAAPPLALPDRFRMEIPFFMTPPGERDAPADLGEGE
                     YWIDPDDARSWLDEGVVYVVSPLDAENKAEIELTEYHEQFLQWLVTHGIRRVRLA"
     gene            complement(3735..>4283)
                     /locus_tag="VFB80_06530"
     CDS             complement(3735..>4283)
                     /locus_tag="VFB80_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN33456.1"
                     /translation="VVFTSDNGPWLTQGEQGGSAGLLKDGKGSTWEGGMREPAIAWRP
                     GTVPAGVVSSELASTLDLLPTFCAQAGIEAPKDRPLDGYDITPALAGGKSPRNEMFYY
                     RGFDLMAVRMGPWKAHYMTQTGYGQATPERPFPPLLFNLEVDPGEQFDRGSRISDVLD
                     QIRTLVEKHKAGMQPAASQLEL"
BASE COUNT          738 a         1462 c         1337 g          746 t
ORIGIN      
        1 tcagcgtcgc ttcgccgatg gcgacgaaga cgcggagcgc cgccagctgc cagaacgact
       61 gcgccagccc gctggccgcg gtcatcgcgc tccacacgag caggcccgcc gcaatcaatc
      121 gcgggcggct agcgcggtcg gcgaccgtcc cgaggaacag gcccgcaacg gcatagacca
      181 cggcaaaggc atacccggcc agcagcgtga tcttgaggtc gtcgagctgc agctgcgtgt
      241 ccgccttgag cggcacttgc aggctggcga ccagctgccg gtcgacgaaa ttgacaacgt
      301 tcaacagcgt gagcagcgcg agtacgaacc aggagtaagc gggcgagtaa cccattcgct
      361 attgtccggc cctcgcgccc gccgctgcga agcattcaca acgcctaccg tgcgagctac
      421 tgttcgtacg ccaagaccag cgcccaggtg gcccagctgc tggcggcggt cgagatgaac
      481 tggtctttcc cgtgcgggaa gcccgtctcg aagtaggctt ggatcggctt gctgcgggtc
      541 gtgacgcgcc acgagccgtc gtcttgctgc tccttcagca ggtaggcgag cccgcgctga
      601 aaggcggcgt cgctcgtcgc catcccgccc gcctcgtgca gcgcgacgag ggcggtgccg
      661 gtggcgtagg cgtcggactc gatcgcagag gccggctcgc cgctgtcgag ctgcgcccag
      721 ccgccgtccg cgcgctgctt cgccatcaat tctttggccg ccgcggcaat ttcttcggcc
      781 ggcgctgcgg ctgcctccaa gccccacagc cggaacaccc gctcttcgtt gtcctgcgcc
      841 ggcgtcttga gcagccattc gcgggcctgc tgcttgcgct tggcgatccg ctcggcttgc
      901 tcggtcgtgc caaaggtgtt gagcccgtac agggcgacgt aggtcgtggt gaagggcgtg
      961 gcctccgtcg gcgggcgctt ggaagtattg ccccagtggt cgcgatcctg atgacgctgc
     1021 agcaggtact cggcgaccgc gccggtcgtc tcgtcgggcg agcagccgcc ggcggccagc
     1081 gtccagagcg cgtaaccggc ggtgtcggcc tgtccgccct ggccctggcc cttgagataa
     1141 ttctcgcggt tgcccgccag gaattttgcc gtgtgagcca gttgcttctt caactcctcg
     1201 tcgtcgatgg caaatccctt gctcttggca gccgtcagcg ccagcacggg cagcccctgg
     1261 ctatggcagg cgaaacaagt gcggctctcg cgatggccga ccgccccctt ggtcaatagc
     1321 ggcagcgact tttcgatcgc ggcgcggatt ctgggctgtt caactgtgat ggggtcagcg
     1381 gccgtcgcag aactggcggc gccaatcagc agaccgagaa cgagccaatg atatcgagtc
     1441 ataaccggac tcctgccgcc acagatggca gcgtgcaata tactccagtg gcgtccgccg
     1501 ggcacacaag tacgtcgtcc tcagtacgaa ctaccaggta ccaacctctg tctcctgtcc
     1561 cctgccacct gtctcctgac ctctgacctc ttgcctcccg acgactccta cgcccaggcc
     1621 ctcgccgccg tcgagcagac gctcgatcgc gtgcggcaat gctcggacga ggagaaggcg
     1681 aagctgcggg ccgagctggc cggcctgcgg cagatgcacg acaagctgac cagcggccgg
     1741 gtcgagatcg tcatcttcgg cgagatcagc accggcaaaa gcgccctcat caacgccctc
     1801 gtcggccgcg aagtcgccag cgtcgatgtc cgcggcggct ggacccgcga agtctggcac
     1861 gtggcctggg acggcacggg ctacacgctg ccgggattgg gcgactcgca agtcgtgctg
     1921 gtcgatacgc ccgggatcaa cgaggtcggc ggcggcccgc gcggcgaaat ggcccgcgag
     1981 gcggcccagc ggtccgacct cgtgctgttc gtcaccgatt ccgacctcaa cgaaaccgag
     2041 catacctccc tcgtcgaatt agccgcgttc cacaagccgc tggtcgtcgt cctcaacaag
     2101 ctcgacctct attcccccga tcagcgcgat aagctgctgg cggtgatccg caaccagcgg
     2161 ctccgcgatg tcgtgccgcc ggagcacgtc atcaccgcgg cggccgatcc gcgcgaacgg
     2221 gagtacgtca ttcaggcggc caatggcagc gagcgcagcg agtggcgcaa gccgccgccc
     2281 gacgtcgccg agctcaaggc cctggtcctg gagctgctcg accgtgaggg gctgtcgctg
     2341 atcgcgctca acgcggcgct ctatgccgcc gacaagagcg accgcgtcgc ggcgctccgc
     2401 gtgcagctcc gcaactcgcg agcccagcag gtgatttgga gctacgccac gctcaaggtc
     2461 atcggcgtgg gcctcaaccc ggtgccgatc gccgacgtgg tcggcggcac ggccatcgac
     2521 gccgctatga tcggcacgct ggcggccgtc tatggtctgc aaatgtctcg cacgcacgcc
     2581 tcgcggctgg cggcctcgat cgcccaggcc gcaggcctga tgacggtcag cgtcgtcgcc
     2641 tcgaccgccc tcatgtcgct gctcaagggg ctgaccttcg gcaaatcgac gctgctcacc
     2701 gcggtgccgc agggcctcgc cggcggcttc ggctcgtaca tcgtcggcca ggccgcccag
     2761 tattatttcg agcacggcgc gagctggggc ggcgaaagcc ccaagtccgt cgccaaaaaa
     2821 atcctcagcc agaccgacaa ggcctccgtt cttactcgcc tcaaaggcga aatcggcaag
     2881 cggctggcga tcaatccgta tgcgaaggga tagaggtcag aggtcaggct cagtactcag
     2941 tacaattatc aacagcccgc aattggtcta acttctaacc tctgaactct aacccctgaa
     3001 ccttgaaccc tcccgtgact gtcacctctc tcgaccagcc cgctgctccg ccgctggcgc
     3061 tgccggaccg gtttcgcatg gagattccgt tcttcatgac gccgccgggc gagcgcgatg
     3121 cgccggctga tctcggagag ggggaatact ggatcgatcc cgacgacgcc cgcagctggc
     3181 tcgacgaggg cgtcgtctat gtcgtctcgc ccctggatgc cgagaataag gccgagatcg
     3241 agctgaccga gtaccacgag cagttcttgc agtggctcgt cacgcacggc atccgccgcg
     3301 tccggctcgc ctaacccgcc tatccccggc ccctggccgg ccggggtggg caatgcgccc
     3361 atactcgaac atttcttcga gcggcagttt tcgaggcaac ccatgacgcc gctggcgctt
     3421 acggcaaaaa atgccgtcca gggattatgc gcattatgcg cgccaattgg gcgaaggcgc
     3481 cgtggccatg cgcgcatact cgccggattt tttctagcgg cgtttttcgc cgcaagctca
     3541 tgcgccatca aacgttgcat tcacaaaacc ggctcagcga ttatgcgcat tatgcgccag
     3601 ggtttccaga acctgcttca cgaccgaatt gtcaaggaac gtcacccgcc gattccacga
     3661 cgaggcccag ccaaaccgcc aattatagca actgtacata taaagtcaag aattgcaaag
     3721 gcgctgggca gcgatcacaa ctccagctga gaagccgccg gctgcattcc cgctttgtgc
     3781 ttctcgacga gcgtccgaat ctggtccagc acgtccgaaa ttcttgaccc ccggtcgaac
     3841 tgctcgcccg gatcgacttc gagattgaag agcagcggcg gaaagggacg ctcgggcgtt
     3901 gcctggccgt agcccgtctg cgtcatgtag tgagccttcc aagggcccat ccggacggcc
     3961 atcaggtcga agccgcgata gtaaaacatc tcgttacgcg gactcttgcc gccggccagg
     4021 gccggcgtga tgtcgtagcc gtcgagtggg cgatctttcg gggcttcgat ccccgcctgg
     4081 gcacagaacg tcggcagcag gtcgagcgtg ctggcgagtt cgctcgaaac cacgccggcc
     4141 ggcacggtcc cgggacgcca ggcgatcgcc ggttcgcgca tgccgccttc ccaggttgat
     4201 cccttgccgt ccttgagcag cccggcggat ccgccttgct cgccctgcgt cagccagggg
     4261 ccgttgtcgc tggtgaagac aac
//
LOCUS       DASRJB010000238        12121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_68351, whole genome shotgun sequence.
ACCESSION   DASRJB010000238 DASRJB010000000
VERSION     DASRJB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68351"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFB80_06535"
     CDS             <1..749
                     /locus_tag="VFB80_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN33457.1"
                     /translation="TDVMEEDQATGKHRVSFMQGPVFTNFLLADEINRTPPKTQAALL
                     QAMQEGEVSVGRETYKLKPPFFVVATQNPIEMEGTYPLPEAQLDRFMFNVRVKYPTVD
                     EEVQIIKGTTSTLTAHADKCLGADEVLRLQELVRGVPIAESVVRYTARFVAATRPGST
                     GSGAKGLDAVHKYLSYGASPRASQYLVLGAKARAILAGKYHVDFADIQAVAAPVLRHR
                     LVLNFHARADNLDADVLVERLLKLVPVEEN"
     gene            753..1613
                     /locus_tag="VFB80_06540"
     CDS             753..1613
                     /locus_tag="VFB80_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33458.1"
                     /translation="MSLRCSSTLLALAAIAVCALGARGDERTDGVRAELLEQMRGLAG
                     QTKLTISAGKMPDLVKNPVFRYDDQPRRFIDATMWLWTVQGRPVAFQKIEAVEYGDAK
                     APSSSWQCCFASASPDLLNVEWPGGRRFRTKEAGIEVRALAGAPSPAEGSLQRKRQGR
                     ELARKFSARIVTSPKNNTRQEMRLLTTPLVEFDDPDSKEYQGAVFAFSTNGTNPDVLL
                     LLEIRSDGQSDKPRWHFAPVRMTCCEVRLAYVDTEVWQVDWVNGTEAPFPTWTFFSTA
                     RAPLAGEAQP"
     gene            1769..2548
                     /locus_tag="VFB80_06545"
     CDS             1769..2548
                     /locus_tag="VFB80_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33459.1"
                     /translation="MAPPAEDKALREQWQRVYQKIAGSIDMRRGETALVLEPAPLLFY
                     TNPVRLNQQHGSIFLWTENGRPVVFGSIWSALNRNDATVRFVTHEFHSLATAPDVTAS
                     KSGQKLWTSGEPGIAWQQLDGAPVPAATRTARLVQLRQIARRITARITTQPEASDLRL
                     MTNPLYRYPDSGIVREGAVFAFSLATDPELILLIDADLAANKPAYKVAFARFGNLAME
                     VKDGDKPIWSCDRGTPGRSEGKYYLRWRAEEMPANPLNPEP"
     gene            2557..3453
                     /locus_tag="VFB80_06550"
     CDS             2557..3453
                     /locus_tag="VFB80_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN33460.1"
                     /translation="MSFDPRQLADPVFFSRLDNLELRAQGIVEGFMHGLHRSPFVGFS
                     VEFASHREYAQGDDLRHVNWKIFARQNRLYVKQFDAETNMNLYLLLDVSGSMDCRSKG
                     ISKLEYGAALAAALGHLALKQHDAVGLILLADEVLAHLPPRARPHQLQEILRIIHNTK
                     PRQTASTARAFQQAAELCTHRGIVVILSDLFDDLDALMQAIERLRFAQHEVVVFHLWD
                     PWERDLPLEGHCKFHDLETGEVLVTQAESVRAGYVAAVEAWRRELETHCRNRSVDRVE
                     LKTDDPLDQALLDYLVRRAKTL"
     gene            3550..5613
                     /locus_tag="VFB80_06555"
     CDS             3550..5613
                     /locus_tag="VFB80_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN33461.1"
                     /translation="MSFLQIGFLGALAALAIPIIIHLVFRQRSKRVDLGTLRFLRVVL
                     QQNARRRRVMRWLLLTLRLACVALLAVLFARPYWLAFRPAGEKQTVAILIDRSATMDV
                     KTDGQRLIERAVAAAKEIVAASGSNTRFEVAFFDHAVRPLAGSYLASGGRESPDNRRE
                     ADASRSPKRDLSPSELLGKLQAPAVCAGGTDYGAVIDWARDVLAKSPAGPRTLHIFTD
                     LQQSGLAWSEVDSLPDDVVTHLHDLGRSAVNNIAVTEARPERTWLRPQEQTAIHVTVH
                     NGGIFTIEELPIQLTLTSGGRRVELKEQVKLEPGTSESVRFDLPPLAEGAWQGSVAVE
                     TEDDLALDNQRHVAFVASPPYQVLLVDGRSAQSPVLAATYFLDTSLRLAPAGEIDAAS
                     LFQPHSILLENGLPALDKFQVVVLADVGELDRGDAAKLARFVDAGGGLLVFGGENVTG
                     PRTAAIAAAGLGVGSVGAVANATDLPLRLGTWDSKHPIFAAFSDPQLGDLARLSFSAC
                     TQLTPAADAQVLARFKDGKPAVIERRRGKGSIVWFASSCDRQWSDWTRSRLYLPLMYQ
                     LLGHQTGYLAGGPVRSAVLEGQVELPVDAQPGVVAKDGFSLVVNTSPRESETERCTSD
                     DFVNRFGLKLAAAGPAAPAPAPAATAKASLGTEMIDSEFWPWIALALLGSLLVEGIVA
                     NRTAA"
     gene            5738..6517
                     /locus_tag="VFB80_06560"
     CDS             5738..6517
                     /locus_tag="VFB80_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33462.1"
                     /translation="MSEFRLLAQEPPAADTREAAQRLELMKSQAAAYEVKLGEPQSPL
                     KLHAEPLLRFSNPVGGVPDGIAVMWKQGARPAIFAQVFQTKEGLWIHECQSLASAPLS
                     MEQGGKVLWSPQEAAPAFQELQGPAAGATPARRLVQMKSLAAEFSASDDFKISSQDKE
                     TTRHELRLLPTPVYRYADSAAGITDGAVFAYVHGTDPEAFLVLELRSDKSGGDSWHYA
                     LAPMTCWAVHVRRGQTEVFSSEERFAKSKPQDLYHVWVHKP"
     gene            6567..10061
                     /locus_tag="VFB80_06565"
     CDS             6567..10061
                     /locus_tag="VFB80_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HZN33463.1"
                     /translation="MSTAPHFVERFDRIWVQARRVQLSQAICWGVLTALAGIALLAAL
                     DYWLELSRPLRLFAIGAIGVGAVAVAVSLVVRSIQRWKRGATAATIEHVFPQLGQRIR
                     TTVQYGELPQEKLQESGVAGTLVEALESDTVRLAQPLPLDAVIPWKSLALASLLAAVV
                     GLALAGASALNWEWRAAARRALAGEEAYTKLTVVPGNASVMEGQSLLVEVNVAGRSGN
                     QLLFQSRRTDDPDSEWRLETLPIDDAEKTGEQQWRYSIPLDRIRHPLEYRVSAGSAKT
                     ETFRVAVRYPLKIVRISAAVQPPAYTRLPEAIVEGSNLAALVGSKAKLEIELDRAAET
                     AWLEMQALPTRGQPTPPVEKIPLAVDGVKLTAGIEIAGDRTFAVFARSADAMELPENK
                     HRIRARQDEPPRVWFESPNEALEVHTLAEILMRIRVSDDFGLSRAGIMFEVNNEDEYP
                     LLMQDFLEAAEELQTTGTLSPSTRATLEKMLPLEHFQLTQRDSVMYYAFAEDILPGGA
                     QRTETDLRFIDIRPFKRTYRLPPDGEPGENMGRQSRLKSLEELIARQRYALNRAIGLA
                     KKFERSGQADLAGTDSLVKFEGELAKFTHDLSVGLVARGVDDVEMLNQAENAMLAATD
                     SLAAGRYDTAEQQMRDAVKNLIDARDRLEIILRNNRNRMQLAALRNFDRTQQQKIRRP
                     KSDEEEAKQIAQRLEELANEEDFVYATLAGLLDPDQTGKAKNPSQGATGDSEKPAPMP
                     MPGESPMPLPAAGNPAEQPAKAGMTREELEDKQLDIGAAAREIEKALARLPRATDLAK
                     ERMTATAKAAEDAGEAIGRGALEDAKSTAGVAREQFRELAQQVRALLADEQADRIAAA
                     QQMAANLARLQEDFVDRLSKPGDNPGLMPADPQQPKNDQPAPGIGQEPPKDPNQKLPG
                     VGAAADKIAEKAKTLADVLGAASRADRPEDEPSAEKVAALAGALDLPSVTERLKNLPG
                     QVREGKKEDARATAGDGAEKMEAAAEQLAGLHRQIVAPRVEELAKLERQVVQLDGQLD
                     ELETDTRVTQWHLSASELLDQVDEKGLAKELTAEFKEEMKKAGWGPDLARGRWNWARI
                     DGGNYQAPARYRPLLSRLAADIRQQMQEMLLGDLRSTGDEPIPPQYEEFVDRYYKVLA
                     TDGKPGARRQDTGDRGQK"
     gene            10061..10873
                     /locus_tag="VFB80_06570"
     CDS             10061..10873
                     /locus_tag="VFB80_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33464.1"
                     /translation="MIGVWLVIGLIGQAPAANPQAAIVEHLHAEYRADAVKCSFFGAA
                     QQPLKLVEKPVMKWANDDDWSGDVFLWTRDSLPAVIGCMLSGPGGGDSRLMFHEFHLL
                     DSRPIAGAELQTRRSWQPAEGLKRQLVANPPPPADTPAARLTQMRQIAKSFTAHMEAD
                     GPWELRLLPQPLFRYGDEAGETIDGAIFTWVWSKGTDPEFLLLVELRPGEKEPAWHYA
                     PVRFSNRELWLKYAGVEVWRAEPHKEPEGGRTSQNYTTAYARGLPRAPAQPD"
     gene            10877..>12121
                     /locus_tag="VFB80_06575"
     CDS             10877..>12121
                     /locus_tag="VFB80_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZN33465.1"
                     /translation="MLFAESTSRFLPPLSAGTLLCAVGAALLALLAARWLLGPANEVA
                     RRWGLWAIRGAILALVAMVLFNPVRVTESPGPTQRPEIFYLLDTSASMQMGAPRSRWD
                     ESLALIRGAQQLAEGARAEVKAFRFGQRLVALDPAAQLGLTEMLAASGVKVTPASLPA
                     ADKSVKPVLPTDADTRMLAALRQISSRFGRVPPQGIVLVSDGRAHDEAGLEQLAAEFA
                     KLKIPIHVAPIGDMTKGGDVAVSAVVAPPRARKFTEVDVQVFVRSFGYDGKRSEVQLW
                     ELADDGQLGRQLCAPLPITLQSGFQSVSLSFRTELHTRKLRVAIPALSDEVSSANNQI
                     DAEMQIERTKIRVLYVEGSQQPLTQVRVGDRYQIRGAFSDLKSALTEDEDIECVVLVT
                     QPGGGFARVAEYATPDGVRGFPT"
BASE COUNT         2127 a         3998 c         4095 g         1901 t
ORIGIN      
        1 gcaccgatgt gatggaagag gaccaggcga ccggcaagca ccgcgtctcg ttcatgcaag
       61 ggccggtctt caccaacttc ctcttggccg acgagatcaa ccgcacgccg ccgaaaacac
      121 aggcggcgct gctccaagcc atgcaggagg gcgaggtctc cgtcggccgc gaaacctaca
      181 agctcaagcc gccgttcttc gtcgtcgcca cgcaaaaccc gatcgagatg gagggcactt
      241 acccgctgcc cgaagcccag ctcgaccgct tcatgttcaa cgtccgggtc aaatatccga
      301 ccgtcgatga agaggtgcag atcatcaagg gcacgaccag cacgctcacc gcccacgccg
      361 acaaatgcct gggcgccgac gaggtgctgc ggctccaaga actggtccgc ggcgtgccga
      421 ttgccgaatc cgtcgtccgc tatacggccc gcttcgtcgc tgcgacgcgg cccggctcga
      481 ccggctccgg ggccaagggc ctggatgccg tgcacaagta cctgtcgtac ggggccagcc
      541 cgcgagcctc gcagtatctc gtgctcggcg cgaaggcccg cgcgatcctg gccgggaaat
      601 accacgtcga tttcgccgac atccaggccg tcgccgcgcc ggtgctgcgg caccgcctgg
      661 tgctcaactt ccacgcccgc gccgataacc tcgacgccga cgtgctggtc gaacggctgc
      721 tgaagctcgt gcccgtggag gagaactaag cggtgagcct gcgctgctcc agcacgttgc
      781 tcgcgctcgc tgcgatcgcg gtctgcgcgc ttggcgcgcg cggcgacgaa cgcaccgacg
      841 gcgtccgcgc ggaattgctc gaacagatgc gggggctcgc cgggcaaaca aagctgacga
      901 tctccgcggg caaaatgccg gacctggtga agaacccggt ctttcggtac gacgatcagc
      961 cgcggcggtt catcgacgct acgatgtggc tctggacggt ccaggggcgt cccgtcgcat
     1021 ttcagaaaat cgaggcggtc gagtacggcg atgcgaaggc gccttcgtct tcgtggcaat
     1081 gctgctttgc gtctgcttcg cccgacctgc tcaacgtgga gtggccaggc ggccgccggt
     1141 ttcgaacgaa ggaagccgga atcgaggtcc gggcgctggc tggcgcgccg tcgccggccg
     1201 aaggcagcct gcagcggaag cgtcaggggc gcgagctggc gcgcaagttc tctgcccgca
     1261 tcgtcaccag cccaaagaac aatactcgcc aagagatgcg gctgttaacg acgccgctgg
     1321 tcgaattcga cgacccggac tccaaggaat accagggagc cgtcttcgcg ttctcgacca
     1381 acggcaccaa tccggacgtg ctgctccttt tggaaattcg cagcgatggc cagagcgaca
     1441 agccgcggtg gcacttcgct ccggttcgga tgacctgctg cgaggtgagg ctggcctacg
     1501 ttgacaccga agtttggcag gtcgattggg tgaatggcac cgaggcgccg ttcccgacct
     1561 ggacgttctt ttccacggct cgcgcgccgc tcgcggggga ggcccagccg tgacgttcgt
     1621 ctctcaaata ctcacacaga ggcgccgagg cgcagagatg agctgtgcac gctcttcctc
     1681 agtgcctctg tgcctctgtg cgagattctc gcttggttgt ttcctctggt tcgtcctttt
     1741 gcgtagtgcc ctcgcccaaa cggaaccgtt ggctcctcct gcggaagaca aagccctgcg
     1801 cgagcagtgg cagcgggtct atcagaaaat cgccggctcg atcgacatgc gccgcggaga
     1861 aacggcgctg gtgctggagc ccgcgccgct gctgttctat accaaccccg tgcggctgaa
     1921 ccagcagcac ggctcgatct tcctctggac ggagaacggc cgaccggtgg tcttcggcag
     1981 catctggtcg gccctcaacc gcaatgacgc gaccgttcgc ttcgtgacgc acgaattcca
     2041 ctcgctggcg acagcgcccg acgtcaccgc cagcaaatcg ggccagaagc tgtggaccag
     2101 cggcgagccg ggaattgcct ggcagcaatt ggacggcgcg cccgttccgg cggccacgcg
     2161 aacggcccgc ctcgtgcagc tacggcagat cgcgcggcga attacggctc gcatcacgac
     2221 gcagcccgag gccagcgacc tgcggctgat gaccaacccg ctctaccgct atccggacag
     2281 cggcatcgtc cgcgagggag cggtgtttgc tttcagcctg gcgaccgatc ccgagctgat
     2341 cctgctgatc gatgccgacc tggcggcgaa caagccggcc tacaaggtcg ccttcgcccg
     2401 cttcgggaac ctggccatgg aggtcaagga cggcgacaag ccgatctggt cctgcgaccg
     2461 cggcacgccc ggccgctcgg agggcaaata ctacctacgc tggcgcgccg aagagatgcc
     2521 ggcgaatccg ctgaaccctg aaccctgaac cctgccttgt ccttcgaccc ccgccaactc
     2581 gccgacccgg tcttcttctc gcggctcgac aacctcgagc tgcgggccca gggcatcgtc
     2641 gaagggttca tgcacggact gcaccgcagc ccgttcgtcg gcttctcggt cgagttcgcc
     2701 tcgcaccgcg aatacgcgca aggcgacgac ctgcggcacg tcaactggaa gatattcgcc
     2761 cggcagaacc gcctctacgt gaagcagttc gatgccgaga cgaacatgaa cctgtatctg
     2821 ctgctcgacg tcagcggctc gatggactgc cgcagcaagg gcatcagcaa gctcgaatat
     2881 ggcgcggcgc tggccgccgc cctgggccac ctggccctca agcagcacga cgcggtcggc
     2941 ctgattctgc tggccgacga agtgctcgcc cacctgccgc cgcgggcccg gccgcaccag
     3001 ctccaggaaa tcctgcggat catccacaac acgaagccgc ggcagaccgc cagcaccgcc
     3061 cgcgcgttcc agcaggcggc cgagctctgc acgcatcgcg gaatcgtggt catcctcagc
     3121 gacctgttcg acgacctgga cgccctgatg caggccatcg agcggctgcg gttcgcccag
     3181 cacgaggtgg tcgtgttcca cctctgggat ccgtgggagc gcgacttgcc gctggagggg
     3241 cactgcaaat ttcacgacct ggagaccggc gaagtcctgg tcacgcaggc cgagagcgtc
     3301 cgcgccggct acgtcgcggc cgtcgaggcc tggcggcgcg agctggaaac gcactgccgc
     3361 aaccgctcgg tcgatcgggt cgaattgaag accgacgacc cgctggacca ggcgctgttg
     3421 gattacctgg tgcggcgggc caaaacacta tgaataccga agaaccgaag aaccaaagaa
     3481 ccaaagaacc acaggggcaa ctcagctccc tcttggttct tcggttcttc ggttcttgag
     3541 attttcctta tgtcgttcct ccagatcggc tttctcggcg ccctcgcggc gctggcgatt
     3601 ccgatcatca tccatctcgt ctttcgccaa aggtcgaagc gagtcgatct ggggacgctc
     3661 cgtttcctcc gcgtggtgct gcagcagaac gccaggcggc ggcgggtgat gcggtggctg
     3721 ctattgacgc tgcggctggc ctgcgtggcc ctgctggctg tgctgtttgc ccgcccctat
     3781 tggctggcct tccggcccgc cggcgagaag cagaccgtgg cgatcctgat cgaccgctcg
     3841 gccacgatgg acgtcaaaac agacggccag cggctgatcg agcgagccgt cgccgcggcc
     3901 aaagagatcg ttgccgcttc gggcagtaac acgcggttcg aggtggcctt cttcgatcac
     3961 gcggtgcggc cgctggcagg gagctatctg gcgagcgggg gacgtgagtc ccccgataat
     4021 cgccgggagg ctgacgcctc ccgctcgcca aaacgcgacc tctcgcccag cgagctgctc
     4081 ggcaagctgc aggcccccgc cgtctgcgcc ggcggcaccg actacggcgc ggtgatcgac
     4141 tgggcccgcg atgtgctggc caagtcgccc gccggcccgc ggacgctgca catcttcacc
     4201 gacttgcagc aaagcggcct ggcctggagc gaggtcgatt cgctgcccga cgacgtcgtt
     4261 acgcacctgc acgacctggg ccgctcggcg gtcaacaaca tcgccgtcac agaggcccgg
     4321 cccgagcgca cctggctgcg gccgcaggag cagaccgcca ttcacgtcac cgtgcataac
     4381 ggcggcatct tcaccatcga agaattgccg atccaactga cgctgaccag cggcggtcgg
     4441 cgggtcgagc tgaaggagca agtcaagctg gagccgggca cgagcgagtc ggtccgcttc
     4501 gacctgccgc cgctggccga aggcgcctgg cagggttccg tggcggtgga gaccgaggac
     4561 gacctcgcgc tcgacaacca gcggcacgtc gccttcgtgg cgtcgccgcc ttaccaggtg
     4621 ctgctggtcg atggccgttc ggcccagtcg ccggtcctgg cagcgaccta tttcctggat
     4681 acctcgctgc ggctggcgcc ggcgggagaa atcgacgccg cgagcctgtt tcagccgcac
     4741 tcgatcctcc tggaaaacgg cctgccggcg ctggacaagt tccaggtcgt cgtgctggcc
     4801 gacgtcggcg aattggaccg cggcgacgcc gccaagctag cccgcttcgt cgatgccggc
     4861 ggcgggctgc tcgtcttcgg cggcgaaaac gtcaccggac cgcggaccgc cgcgattgcc
     4921 gctgccggcc tgggcgtggg aagcgtcggg gccgtggcca atgcgaccga cctgccgctg
     4981 cgcctgggga cgtgggactc caagcacccg atcttcgccg cgttcagcga tccgcagctc
     5041 ggcgacctgg cccggctctc cttttcggct tgcacgcagc tcacgccggc ggcggacgcg
     5101 caggtgctgg ccagattcaa ggacggcaag ccggcggtca tcgagcgccg ccggggcaaa
     5161 ggatcgatcg tctggtttgc ctcgagctgc gaccggcagt ggagcgattg gacccgcagc
     5221 cggctctatc tgccgctgat gtatcagctg ctcggccacc agacggggta cctggccggc
     5281 ggtccggttc gcagcgccgt gctcgaaggg caggtcgaat tgcccgtcga tgcccagccc
     5341 ggcgtcgtcg ccaaggatgg attttccctg gtcgtgaaca ccagcccgcg cgagtcggag
     5401 accgagcgct gcaccagcga cgacttcgtc aaccgttttg gcctgaagct cgccgccgcc
     5461 gggcctgccg ccccggcgcc cgcgccggcg gccacggcca aggcgtcgct gggaacggaa
     5521 atgatcgaca gcgagttctg gccctggatc gccctggcgc tgctgggatc gctgctcgtg
     5581 gaagggattg tcgccaaccg cacagccgcg tgagaaaagc tcgccgtacg gaacaggttc
     5641 gcagcacgaa ttcgaaatgt atcaaaccca atcatttagc ccgtttcggt cggtggcact
     5701 tcagtcagtg gtgcttgttt cgtgcttcgt gcttcggatg tcggaattca ggctgttggc
     5761 ccaggagcct ccagccgcgg atacccggga ggctgcccag cgcctggagc tgatgaaatc
     5821 ccaagcggcg gcctatgagg tcaagttagg cgagccgcag tcgcccttga agctgcacgc
     5881 ggagccgctg ctgcggttca gcaatccggt cggcggcgtg ccggatggca tcgcggtgat
     5941 gtggaagcag ggcgcgcggc cggcgatttt cgcgcaggtg tttcagacga aggaaggcct
     6001 gtggatccac gagtgccagt cgctggccag cgccccgctg agcatggagc aaggcggtaa
     6061 agtcctctgg tcgccccagg aggccgcgcc ggcgtttcaa gaactccagg gccccgccgc
     6121 aggcgcgacg cccgcccgcc gattggtgca gatgaaatcc ctcgccgcgg agttttccgc
     6181 gagcgacgac ttcaagatca gctcgcagga caaggaaacg acgcggcacg agctgcgtct
     6241 gctcccgacg cccgtttatc gctacgccga ttccgccgcg ggaatcaccg acggggccgt
     6301 gttcgcctac gtgcatggga ccgaccccga ggcgtttctc gtgctggaat tgcgtagcga
     6361 taagtcgggc ggggacagct ggcactacgc cctagccccg atgacctgct gggccgtgca
     6421 tgttcgccgc ggccagaccg aagtcttcag cagcgaagag cggttcgcca agagcaagcc
     6481 gcaggatttg tatcacgtgt gggtacacaa gccctgacgg ccatccaaca actcagcaga
     6541 tttccaacca atcccgagat tcagccatgt ccaccgcgcc gcattttgtc gagcgtttcg
     6601 atcgcatctg ggtgcaggcc cgccgcgtgc agctctcgca ggcgatctgc tggggcgtgc
     6661 tcacggccct ggcgggaatc gctctgctgg cggccctcga ttactggctg gagctgtctc
     6721 ggccgctgcg gctgtttgcg atcggcgcga ttggcgtcgg cgcggtcgcg gtggccgtgt
     6781 cgctggtggt gcggtcgatc cagcgctgga agcgcggcgc gacggcggcg acgatcgagc
     6841 acgtctttcc gcaactgggc cagcgaattc gcaccacggt gcagtacggc gaattgccgc
     6901 aagagaagct gcaggagtcg ggcgtcgcgg gcacgctagt cgaggccctg gagagcgata
     6961 cggtccggct cgcgcagccg ctgccgctgg acgcggtgat tccctggaag tcgctggccc
     7021 tggcgtcgct cctggcggcg gtcgtcggcc tggcgctggc cggcgcatcg gcgctcaact
     7081 gggaatggcg ggccgccgcg cggcgggccc tggcgggtga agaggcctac acgaagctca
     7141 ccgtcgtgcc cggcaatgcc tcggtaatgg aggggcagtc gctgctggtg gaggtgaacg
     7201 ttgccggccg tagcggcaat cagctcctgt tccagtcgcg gcgcaccgac gacccggaca
     7261 gcgagtggcg gctggagacg ctgccgatcg acgacgccga gaaaaccggc gagcagcagt
     7321 ggcgctattc gatcccgctc gaccggattc gccatccgct ggaataccgc gtcagcgccg
     7381 gctcggcgaa gaccgagacg ttccgcgtcg ccgttcgcta tccgctgaag atcgtgcgga
     7441 tctcggccgc cgtgcagccg ccggcctata cccggctgcc ggaagcgatc gtcgaaggct
     7501 cgaacctggc ggcgctggtc ggctcgaagg cgaagctgga gatcgagctg gaccgcgcag
     7561 cggaaaccgc ctggctggaa atgcaggccc tgccgacgcg cggccagccg acgccacccg
     7621 tggagaaaat cccgctcgcc gtggacggcg tcaagctgac tgcgggcatc gagatcgccg
     7681 gcgaccgcac gtttgcggtg tttgccaggt cggcggatgc catggagctg cccgagaaca
     7741 agcaccgcat ccgcgcccgg caggacgagc cgccgcgagt ctggttcgag agtccgaatg
     7801 aagccttgga ggtgcacacg ctggcggaaa tcctgatgcg gattcgggtc agcgacgact
     7861 tcgggctgtc gcgggccggg atcatgttcg aggtcaacaa cgaggacgaa tatccgctgc
     7921 tgatgcagga tttcctggag gcggccgaag agttgcaaac gacgggcacg ctctcgccca
     7981 gcacgcgggc cacgctcgaa aaaatgttgc cgctggagca cttccagctc acgcagcgcg
     8041 acagcgtgat gtactacgcc ttcgccgagg acattctgcc cggcggggcg cagcggaccg
     8101 agacggacct gcggttcatc gacatccgcc cgttcaagcg gacgtaccgt ctgccgcccg
     8161 atggcgagcc cggcgagaac atgggccgtc aatcgcggct gaagtcgctg gaggaactga
     8221 tcgcccggca acggtacgcc ctcaatcggg cgatcgggct ggccaagaag ttcgagcgta
     8281 gcggccaggc cgacctggcc gggaccgact cgctggtcaa gttcgagggc gagctggcga
     8341 aattcaccca cgatctttcg gtcgggcttg tcgcccgcgg cgtggacgac gtcgagatgc
     8401 tcaaccaggc cgaaaacgcg atgctggcag ccaccgactc gctcgcggcc ggccggtacg
     8461 acacggccga acagcagatg cgcgacgccg tgaagaacct gatcgacgct cgcgaccggc
     8521 tggaaatcat tctcaggaac aatcgcaatc gcatgcagct cgcggcgctc cgcaatttcg
     8581 accggacgca gcagcagaag attcgccgcc ccaagagcga cgaggaagag gccaagcaaa
     8641 ttgcgcagcg gctggaagag ctggccaacg aggaagactt cgtctatgcg acgctggccg
     8701 gactgctcga tccagatcag acgggaaagg cgaagaatcc atcccagggc gcgaccggcg
     8761 attccgagaa gcccgctccg atgccgatgc ccggtgagtc gccgatgccg ctgccggccg
     8821 caggcaatcc agcggaacaa ccggcgaagg ccggcatgac ccgcgaggaa ctggaggaca
     8881 agcagctcga tatcggagct gccgcgcgag agatcgaaaa ggcgctggcc aggctgcccc
     8941 gggcgacgga cctggccaag gagcggatga ccgccacggc caaggctgcc gaagacgccg
     9001 gcgaagcgat cggccgcggc gcgctcgagg acgcaaagag cacggccggc gtggctcgcg
     9061 agcagttccg cgagctggct cagcaagtcc gggcgctgct ggccgacgag caagccgacc
     9121 ggattgccgc cgctcagcag atggccgcca acctggcgcg cctgcaggaa gatttcgtcg
     9181 atcgcctctc gaagccgggc gataacccag gcctgatgcc agccgatccg cagcagccca
     9241 agaacgatca gcccgcgcct ggaatcggcc aggagccgcc gaaggacccg aaccagaagc
     9301 tgccgggcgt gggggctgcg gccgacaaga ttgccgagaa agccaagacg ctggctgacg
     9361 tgctcggtgc ggcgtcccgc gccgatcggc ccgaggatga accctcggcc gaaaaggtgg
     9421 cggcgctcgc cggggctctc gatttgccct cggtcaccga gcggctgaag aacctgccgg
     9481 gccaggtccg cgagggcaag aaggaagacg cccgcgcgac cgccggcgac ggggcggaga
     9541 agatggaggc cgccgccgag cagctggccg gcttgcaccg gcaaatcgtg gccccccgcg
     9601 tcgaagagct ggccaagctg gagcggcagg tcgtgcagct cgacggccag ctcgacgagc
     9661 tcgaaaccga cacccgcgtc acgcagtggc acctctcggc gagcgagctg ctggaccagg
     9721 tcgacgagaa gggactcgcc aaggagctga ccgcggaatt caaggaagag atgaagaagg
     9781 ccggctgggg gcccgacctc gcacgcggcc gctggaactg ggcccgcatc gacggcggca
     9841 actaccaggc tccggcccgc tatcgcccgc tgctgtcccg gctggcggcc gacatccgcc
     9901 agcagatgca ggagatgctc ctgggcgact tgcggtccac gggcgacgag ccgattccgc
     9961 cccagtacga agagttcgtg gaccggtatt acaaggtgct ggcgacggat gggaagccgg
    10021 gagcgaggcg acaggacaca ggggacaggg gacagaagta atgattggag tttggctggt
    10081 aataggattg atcggccaag cgcctgcggc gaatccgcag gcggcgatcg tggagcattt
    10141 gcacgccgag tatcgggcgg atgcggtgaa gtgctcgttt ttcggcgctg cgcagcagcc
    10201 gctgaagctc gtcgaaaagc ccgtgatgaa gtgggccaac gacgacgact ggtccggcga
    10261 cgtctttttg tggacgcgag atagcctgcc cgccgtcatc ggctgcatgc tttcgggtcc
    10321 tggcggcggc gacagccgcc tgatgtttca cgaatttcac ttgctcgata gccggccgat
    10381 cgccggggct gaactgcaga cgcggcgcag ctggcagccg gcggaagggc tcaagcggca
    10441 actggtggcc aatccgcctc cgcccgctga tacgccggct gcccgcctga cccagatgcg
    10501 gcagatcgcc aagagcttca cggctcacat ggaggccgac gggccgtggg aattgcggct
    10561 cttgccgcag ccgctgtttc gctacggcga cgaggccggc gagacgatcg acggcgcgat
    10621 attcacgtgg gtctggagca agggaaccga tccagagttc ttgctgctgg tcgaactgcg
    10681 gccaggtgaa aaggagccgg cgtggcacta tgctcccgtg cgattctcga accgggagct
    10741 gtggttgaag tacgccggtg ttgaagtctg gcgggccgag ccccataaag aaccagaagg
    10801 cggccgcact tcgcaaaact acacgaccgc ctacgcccgc ggtttgcccc gcgcgcccgc
    10861 gcagcccgat tgagccatgc tgtttgccga atcgacatcg cgatttctgc cgccgctctc
    10921 tgcgggcacg ctgctttgcg cggtcggggc ggcgctcttg gccctgctcg cggcccgctg
    10981 gctgctggga ccggccaatg aggtcgcgcg tcgatgggga ttgtgggcga tccgcggggc
    11041 gatcctggcc ctcgtggcga tggtactgtt taatccggtc cgcgtgacgg aaagcccggg
    11101 gccgacgcag cggccggaga tcttctacct gctcgacacg tcagccagca tgcagatggg
    11161 cgctccgcgc tcgcggtggg acgaatcgct ggccctgatt cgcggggcgc agcagcttgc
    11221 cgagggcgcg cgggccgaag taaaagcctt ccgcttcggc cagcgcctgg tggccctcga
    11281 ccccgcggcc cagctgggcc tgacggaaat gctggcggcc agcggagtca aggtcacgcc
    11341 ggcgtcgctg ccggccgccg acaaaagcgt taaaccggtg ctgccgaccg acgccgacac
    11401 gcggatgctg gcggccctgc gccagatcag cagccgcttt ggccgcgtgc cgccgcaggg
    11461 aatcgtgctc gtctccgacg ggcgggcgca cgatgaggca ggcctggaac agctggcggc
    11521 agagttcgcg aagctgaaga ttccgatcca tgtcgcgccg atcggcgaca tgaccaaagg
    11581 gggcgacgtg gccgtctcgg cggtcgtcgc gccgccgcgg gcccgcaagt tcacggaagt
    11641 ggacgtgcag gtcttcgtcc gcagctttgg ctacgacggc aagcgcagcg aagtacagct
    11701 gtgggagctc gccgacgatg ggcagctcgg ccggcagctc tgtgcgcccc tgccgatcac
    11761 gctccagtcg gggtttcaat cggtcagcct ctcgttccgc acggaactgc acacccgcaa
    11821 gctccgcgtg gcgattcccg cgctcagcga cgaggtgagc agcgccaata accagatcga
    11881 cgccgagatg cagatcgagc ggacgaagat tcgcgtgctc tatgtcgagg ggagccagca
    11941 gccgctgacg caagtccgcg tgggcgaccg ctaccagatt cgcggcgcgt tttccgacct
    12001 gaagtcggcc ctcaccgaag acgaggacat cgaatgcgtc gtgctggtga cgcagcccgg
    12061 cggcggattt gcgcgggtcg ccgagtacgc cacgcccgac ggcgtgcgcg gcttcccgac
    12121 g
//
LOCUS       DASRJB010000239         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2605529, whole genome shotgun sequence.
ACCESSION   DASRJB010000239 DASRJB010000000
VERSION     DASRJB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605529"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFB80_06580"
     CDS             <1..823
                     /locus_tag="VFB80_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33466.1"
                     /translation="QSDYGRFGLNWGYGSSPLLYDGKVIVEVLHGNNTDDPSYLVAID
                     GATGKNLWRIERETDAPRESPDAYTTPALLMVNGQAQIVVSGGDYVTGHDPATGKELW
                     RAAGLNPQKAGNYRIVASPVVSGDLIFAPTRNRPLLALKAGGSGDITESHTAWKWEEQ
                     GGPDVPTPVCDGKYFYMVADNGLLTCLDARSGKVVYGPQRLNVGATISASPLLADGKL
                     YITGENAATVVLAAGPEYKPLATNPLDGTFTLSSIAVSGKQLFLRTSTHLYCIGL"
     gene            854..2896
                     /locus_tag="VFB80_06585"
     CDS             854..2896
                     /locus_tag="VFB80_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HZN33467.1"
                     /translation="MRTNSIRPLAALAAGCVVLVSAASAQDARPTLFVVGDSTVKTPT
                     YGQQGWGDPLIAMFDPAKIKVENHAIGGRSSRTFQTEGRWDRILAAAKEGDFVLVQMG
                     HNDGIAPDDPLRPRGTLRGIGEETREITHPRSGQKEIVYTYGWYLRKYVNDAKGKGMT
                     PILVSPIPHCPRQPVEKGQRENNNYVRWTEEVAKAEKVPFIDLNLITMNHYATLTPAA
                     IKEKYFTPGSDNTHTSPAGAERNAQSVVEGIRGLSDSPLAKFLLTDAQAATIGRALPA
                     APNTATAVAEGAAPKDWIDARTGHRIIRLSDDDGGSSLYFHQNSYTPEGDKLILDTRK
                     GIVAVDLAKLGKEPPQPQLIVPEGRALDFARKTRELYFHRFGDLCAANVDTKEVRVVA
                     KGIRATDVNCDETYIVGTASAEDPTGKTPRPEPRTLLPQRERMFGAKLKQGFALSPAE
                     ETSARKEDGLARRLRDPRSQAFVFTSIKTGEANTVGYQYAWLNHLQFSPTDPNLLLYC
                     HEGTWHEVDRIWTIRTDGTQQKLMHKRTIDMEIAGHEFWSHDGRTIWFDLQTPRSQEF
                     WLAGIEVVTGKTTRYKLDRDRWSVHFNVSRDGKLFAGDGGDPGQVAFAVDGQWIYLYR
                     PQADGTLAWERLADLSKHNYRLEPNVTITPDSRWVVFRSNMHGPTHVYAVEITKSP"
     gene            2973..>3265
                     /locus_tag="VFB80_06590"
     CDS             2973..>3265
                     /locus_tag="VFB80_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33468.1"
                     /translation="MPDRSTDRTLPLLLLLFVGSGCAALIYEIVWFQLLQLVIGSSAV
                     SLGVLLGTFMGGMCLGSLLLPRVIGRDRHPLAAYAYLELGIGAIGIAALFVVPL"
BASE COUNT          625 a         1109 c          997 g          534 t
ORIGIN      
        1 gcagagcgac tacggacgct tcggtctcaa ctggggctat ggcagctcgc cgctgctcta
       61 tgacggcaag gtcatcgtcg aggtgctgca cggcaacaac accgacgatc cctcgtacct
      121 cgtggccatc gacggcgcga ccggcaagaa cctctggcgg atcgagcgcg agaccgacgc
      181 cccccgcgaa tcgcccgacg cctacacgac gccggctctg ctgatggtga acggccaggc
      241 tcagatcgtc gtcagcggtg gcgactatgt caccggccac gacccggcga ccggcaagga
      301 actgtggcgg gccgccggcc tcaatccgca gaaggccggc aactaccgca tcgtcgcgtc
      361 gccagtcgtg tctggcgatt tgatcttcgc gccgacccgc aaccggccgc tgttggcact
      421 gaaagccggc ggcagcggcg acatcaccga atcgcacacc gcctggaaat gggaggagca
      481 gggcgggccg gacgttccca cccccgtctg cgacggcaag tatttttaca tggtcgccga
      541 caacggcctg ctcacctgcc tcgacgccag gtcgggcaag gtcgtctatg gcccccagcg
      601 gctgaatgtc ggggcgacga tcagcgcttc gcccctcttg gccgacggca agctctacat
      661 caccggcgag aacgccgcga cggtcgtcct cgccgccggt cccgaatata agccgctggc
      721 caccaatccg ctcgacggca cgttcacctt gtcttcgatc gccgtctccg gcaaacaact
      781 tttcctccgc acttcgacgc acctgtactg catcggcctg tagcgacgtt gacagagtcg
      841 ccgttgctct accatgagaa ccaactccat tcgcccgctg gcggcgctcg ccgccggctg
      901 cgtcgtcctt gtctccgctg catcggccca ggatgccagg ccgacattgt ttgtcgttgg
      961 cgattcgacg gtgaaaacgc cgacctacgg ccagcagggc tggggcgacc cgctcatcgc
     1021 gatgttcgat ccggcgaaaa tcaaggtcga gaaccacgcc atcggcggcc gcagcagccg
     1081 cacgtttcag accgaaggcc gctgggatcg catcctcgca gccgccaaag agggcgattt
     1141 cgtgctggtg cagatgggcc acaacgacgg catcgccccc gacgatccgc tgcggccgcg
     1201 cggcacgctc cgcggcatcg gcgaagagac ccgcgaaatc acccacccgc ggtcgggaca
     1261 gaaggaaatc gtctacacgt acggctggta cctgcggaaa tatgtcaatg acgccaaggg
     1321 caaggggatg acgccgatcc tcgtctcgcc cattccgcac tgcccgcggc agcccgtgga
     1381 aaaaggtcag cgcgaaaaca acaactacgt ccgctggact gaggaggttg ccaaggccga
     1441 gaaagtgccg ttcatcgacc tgaacctgat cacgatgaac cattatgcga cgctcacgcc
     1501 ggccgcgatc aaggagaagt acttcacgcc tggcagcgac aacacgcaca ccagccccgc
     1561 cggcgccgag cgcaacgccc agtcggtcgt cgaggggatc cgcgggctgt ctgattcgcc
     1621 gctggcgaaa ttcctgctga ccgacgccca agccgcgacc attggtcgcg ctttacccgc
     1681 tgcgccgaac acggcgaccg ctgtggctga aggcgctgct cccaaagact ggatcgacgc
     1741 cagaaccggc catcgcatca tccgcctttc cgacgacgac ggcggtagca gcctctattt
     1801 ccaccagaac agctacacgc ccgaaggcga caagctgatc ctcgacacgc gaaagggaat
     1861 cgttgccgtc gatctggcaa agctcggcaa agagccgccg cagccgcagc taattgtccc
     1921 ggagggccga gcgctcgact ttgcccggaa aactcgcgag ctctatttcc atcgcttcgg
     1981 cgacctgtgc gcggcgaacg tcgataccaa ggaagtgcgc gtcgtggcga agggcatccg
     2041 cgcgaccgac gtcaactgcg acgagacgta tatcgtcggc acggcgagcg ccgaggatcc
     2101 aaccggcaag acgccgcggc ccgagccgcg cacactcctg ccgcagcggg agcggatgtt
     2161 cggcgcaaag ctgaaacagg ggtttgcact ctccccggcc gaagaaactt ccgcccgcaa
     2221 ggaggacgga ctggcccgcc gcctccgcga tccgcgctcg caggcgttcg tcttcacgag
     2281 catcaaaacc ggcgaggcga atacggtggg ctatcaatac gcctggctga accatctgca
     2341 gttctcgccg accgatccga acctgctgct ctactgccac gagggcacct ggcacgaggt
     2401 ggaccgcatc tggacgatcc gcaccgatgg cactcagcaa aagctgatgc acaagcggac
     2461 gatcgatatg gagatcgccg gccatgagtt ttggagccac gacggccgga cgatctggtt
     2521 cgacctccag acgccgcgca gccaggagtt ctggctggcc ggcatcgaag ttgtcaccgg
     2581 caaaacgacg cggtacaagc tcgaccgcga ccgctggtcg gtgcacttca acgtttcgcg
     2641 tgacgggaag ctgtttgccg gtgacggcgg cgatcccggc caggtcgcct tcgccgtgga
     2701 cggccagtgg atttatctct accggccgca ggcggacggc acgctcgcct gggagcggct
     2761 ggccgacctg tccaagcaca actaccgcct ggaaccgaac gtcaccatca ctcccgacag
     2821 ccgctgggtc gtcttccgct ccaacatgca cgggccgacg cacgtgtatg ccgttgagat
     2881 caccaaatcc ccgtaaacgt cggtgtcagt ggtcagtggt ccgttggaat accactgcca
     2941 actgaccacg gacgactgac tactgacaaa taatgcccga ccgttcgacc gaccgcacgc
     3001 tgccgcttct cttactcctc tttgtgggca gcggctgcgc ggcgctgatc tacgaaatcg
     3061 tctggtttca actcttgcag ctcgtgatcg gctcgtccgc cgtgtcgctg ggagtactgc
     3121 tcggcacgtt catgggcgga atgtgcctgg ggagcctgtt gctgccgcgg gtgatcggcc
     3181 gggaccggca tccgctggcg gcgtacgcgt acctggagct gggcatcggc gcgatcggca
     3241 tcgcggcgct gtttgtcgtg ccgct
//
LOCUS       DASRJB010000240         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3451377, whole genome shotgun sequence.
ACCESSION   DASRJB010000240 DASRJB010000000
VERSION     DASRJB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3451377"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1149)
                     /locus_tag="VFB80_06595"
     CDS             complement(43..1149)
                     /locus_tag="VFB80_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN33469.1"
                     /translation="MSSIRFLMLGGFLGAGKTTAIARLARHYQQQGKKVGIVTNDQAT
                     DLVDTNSLRAQGFDVGEVPGACFCCNFNKLTETVGQLEKATRPDIVLAEPVGSCTDLV
                     ATVARPLQQLFAAKFDVAPYAVLLKPSHGSRILRGEAKGGFSPQAAYIFKKQLEEADL
                     IVINRIDELSAADATSLAGLLAAQYPTTPVLRMSAKTGAGFDALIEFVDQRGRFGQRL
                     MDVDYDTYAIGEAELGWLNSSLSIAAPQALDLDALLLDIISRLQTSLAAIDAETAHLK
                     AIGMADGAYGVANLVSSFSQPELSLPSRAQVKEAQLVVNARVATAPETLTQQVESAVR
                     AACAARRAAPTFHQTQSFRPGRPVPTHRILQPTG"
     gene            1435..2475
                     /locus_tag="VFB80_06600"
     CDS             1435..2475
                     /locus_tag="VFB80_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33470.1"
                     /translation="MKRTWLTYVAITASLIAGFLCSTAFADNKGMKFSGGSTRSFNSG
                     ATQQRTMQFQQSNNTLRNLGNIQGVQTQIHGGSFNGGQLGQILGNGNGGGKPQIKINP
                     GTQIFNGQGNSQGNANGIKIGGTGIKIGGTNNNGLGITLGNSNVRNAIGQFQGGGNSS
                     KKCYIAPKCIDPCFSKGCSPCGNSCSPCLKNWNVHCTPWWWTCHTHYCNQPIYYVCSQ
                     PIVVTVPVVVGVPVVQEQLVQVPVGSTITMQALGLGDVAGRLILVMDKVSLEGQINDW
                     KLDAVTATLPMFSLAAPTLAELVIVKADGQPATSMKIQLIPAQPAAVGAVQQAQAIDP
                     AGELSAAAALAR"
     gene            complement(2599..3354)
                     /locus_tag="VFB80_06605"
     CDS             complement(2599..3354)
                     /locus_tag="VFB80_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33471.1"
                     /translation="MLARTCLALLASSLAAAGGCCAPHYGGDCCLCLPKPIVWDGSCN
                     ECAPDCESCADCCGDCGILSFLRRKKICGMGCGEIYLGEWISDPPDCCDPCDQCTGCW
                     TGPQGECCLGPCQRLLAACHGYRYCPKPSCGPECGGFCHRGSCGPGCGCDGSPHGAML
                     SGGMPYGAAISEDVLPAPAYGKLSPMYESHHSIMDENWNIPRAKPIPGKPIHKAQQPP
                     TRQMSGGGRPQMSRIVQNRPAIGTGVQQANYAR"
BASE COUNT          636 a         1108 c         1186 g          611 t
ORIGIN      
        1 gcacgacctg cgcggcgttg gggcgcgacg ctcaagcgcg cctcaccccg tcggctgcaa
       61 aatgcggtgg gttggcaccg ggcggccggg gcggaaggat tgggtctggt ggaacgtcgg
      121 cgcggcgcgg cgggctgcgc aggcggcgcg gacggcggac tcgacttgct gggtcagtgt
      181 ttccggtgcc gtggcgacgc gagcgttgac caccagctgc gcttccttca cctgcgcgcg
      241 cgaaggcagt gaaagctccg gctgcgagaa gctgctgacg aggttcgcca cgccatacgc
      301 tccgtcggcc atgccgatcg ccttgaggtg ggccgtttcg gcgtcgattg cggccagcga
      361 cgtttgcagc cgcgagatga tgtcgagcag caaggcgtcg agatcgagtg cctggggcgc
      421 tgcaatcgag aggctgctgt tgagccagcc gagctcggcc tcgccgatgg cgtaggtgtc
      481 gtaatcgacg tccatcaatc gctggccgaa gcggccgcgc tgatcgacga actcgattaa
      541 ggcgtcgaat cccgcgccgg tcttggccga catccgcagc acgggggtcg tcggatactg
      601 agccgcgagc aggccggcga gcgacgtggc atcagccgca gacagctcgt cgatccggtt
      661 gatgacgatc aggtcggcct cctcgagctg cttcttgaag atgtaggccg cctgcggcga
      721 gaagccgccc ttggcctcgc cgcggagaat gcggctgccg tggctgggct taagcaagac
      781 cgcatacgga gcgacgtcga actttgccgc gaagagctgc tggagcggcc gcgcgacggt
      841 cgccaccagg tcggtgcagc tccccaccgg ctcggccagc acgatgtcgg gccgggtggc
      901 tttctcgagt tggccgacgg tctcggtcag cttgttgaag ttgcagcaga agcacgcgcc
      961 gggcacttcg cccacgtcga agccctgcgc ccgcaggctg ttggtatcga cgaggtcggt
     1021 cgcctggtcg ttggtgacga tgccgacctt tttgccctgc tgttgatagt gccgggccag
     1081 gcgggcgatc gcggtcgttt tgccggcacc gaggaagccg ccgagcatga ggaagcggat
     1141 ggaggacatg gtggtctggg acaggaggca ggggacagaa tcacacgggg aaaatcgttg
     1201 tcatagttcg ccgagtctta cgctgtgcct gataggcggc gcgatctgcg ccatcggttt
     1261 cagtcagccc tcgcggcagc cgttttccgg ctgttccggc cgcggcacgt tcgtacgact
     1321 ctttgagttt ccacctgggt aacattctgc ccagtttcac gcttcgctgg gtagaggcca
     1381 aacgaatttg gcccgcaaga atcgacctaa acctaagtct gaaaggactt tacaatgaaa
     1441 cgcacctggc tcacgtacgt cgcgatcacg gcttcgctga tcgccggttt tctctgcagc
     1501 accgcgtttg cagacaacaa gggcatgaag ttcagcggcg gctcgacccg ctcgtttaac
     1561 agcggcgcga cgcagcagcg cacgatgcag ttccagcaga gcaacaacac gctccgcaat
     1621 ctcgggaaca ttcaaggcgt ccagacgcag atccacggcg gcagcttcaa cggcggccag
     1681 ctgggtcaga tcctgggcaa cggcaacggc ggcggcaagc cgcagatcaa gatcaacccc
     1741 ggcacgcaga tcttcaatgg ccaaggcaat agccaaggca atgcgaacgg catcaagatc
     1801 ggtggcaccg gcatcaagat cggtggcacg aacaacaacg ggctgggcat cacgctgggc
     1861 aacagcaatg tccgcaacgc gatcggccag ttccagggcg gcggcaacag ctcgaagaag
     1921 tgctacatcg ccccgaagtg catcgacccg tgcttcagca agggttgcag cccctgcggc
     1981 aactcgtgca gcccgtgcct caagaactgg aacgtgcact gcacgccctg gtggtggacc
     2041 tgccacacgc actattgcaa ccagccgatc tactacgtct gcagccagcc catcgtggtc
     2101 acggttccgg tggtggtcgg agtcccggtg gttcaggagc agctggtgca ggttccggtg
     2161 ggttcgacga tcactatgca ggccctgggc ctgggtgatg tcgcgggccg cctgatcctg
     2221 gtgatggaca aggtctcgct ggaaggccag atcaacgact ggaagctcga cgcggtgacc
     2281 gcgacgctgc cgatgttcag cctggctgcc ccgaccctgg ccgagctggt catcgtgaag
     2341 gccgacggtc agcccgcgac ctcgatgaag atccagctga tcccggctca gcccgctgcc
     2401 gtcggtgcgg ttcagcaagc ccaggccatc gatccggccg gcgagctgtc ggccgccgcc
     2461 gccttggctc ggtagctata cggcccggta aataaacagc ttgggaacga tcaacccaag
     2521 ccacaaaaag cagccggagg caaaaacctc cggctgcttt gctttcttga tcgttgccga
     2581 cgcagcttgc gtcggcgctt accgcgcgta gttcgcctgc tgcacaccgg ttccgatcgc
     2641 cgggcggttc tggacgatgc gcgacatttg cggccgcccg ccgccgctca tttgccgggt
     2701 cggcggctgc tgggccttgt ggatcggttt gccgggaatc ggtttggccc gcgggatgtt
     2761 ccagttctcg tccatgatgc tgtgatgcga ttcgtacatc ggcgagagct tgccgtaagc
     2821 cggcgccggc aagacgtctt ccgagattgc cgcgccgtag ggcatgccgc cgctcagcat
     2881 cgcgccgtgc ggcgagccgt cgcagccgca acccggcccg cacgagccgc ggtggcaaaa
     2941 gccgccgcac tccggcccac agctgggctt ggggcagtat cgatagccgt gacatgcggc
     3001 cagcaatcgc tggcacggtc cgaggcagca ctcgccttgc ggtcccgtcc agcagcccgt
     3061 gcactggtcg cagggatcgc agcagtcggg cggatcactg atccattcgc cgagatagat
     3121 ttcgccgcaa cccatgccgc agattttctt gcgccgcagg aacgagagga tgccgcaatc
     3181 gccgcagcaa tcggcgcacg actcgcagtc gggtgcgcat tcgttgcagc tgccgtccca
     3241 gacgattggc ttgggcaggc agaggcagca atcgccgccg taatgcggcg cgcagcaacc
     3301 gccggcggct gccagcgacg acgcgaggag ggcgaggcag gtgcgagcca gcatggccgt
     3361 tcgtccggga ggtccgaggg gaagagcagc gatcgctgtt ggttatcgac cggcaaagtt
     3421 ggcccagtcg agaaattcgc cgtaaccggc agattttgcc gcgttcctgc tagcggggcc
     3481 ggcggaatcg aagacgatgg caccattggc gagcaccatg aaaacgcctg ctccacctcc
     3541 c
//
LOCUS       DASRJB010000241         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_350937, whole genome shotgun sequence.
ACCESSION   DASRJB010000241 DASRJB010000000
VERSION     DASRJB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350937"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFB80_06610"
     CDS             complement(<1..390)
                     /locus_tag="VFB80_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN33472.1"
                     /translation="MLPLPPNQHLAAPGKWPIVGERQPADGCGPWKVELSGLVARPLE
                     FSLGELSELLQVERLIDVHCVTRWSKLSCRFSGVPLLALLDRAQPRSDARFVSFVAHS
                     PRQHSTSLPLDVVRRLDPLVALKFEGQP"
     gene            complement(405..3317)
                     /locus_tag="VFB80_06615"
     CDS             complement(405..3317)
                     /locus_tag="VFB80_06615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZN33473.1"
                     /translation="MAHPSVRLWSVSATGVLILAGGVLSLAGCSAEAQPVASNAPKYR
                     PVDAAPADSGSADTAVASELARLNTDLRSGGLDGSASANAGSPTPIAGNVSPRTGSAN
                     ANSATAPAVGKAPTGELKQVLDQIDKLARQSQAPPGTTQQEQIQNFLKIQQQRLLACE
                     KALTLKPDAKTKSVIYQAMYEIHRIFARVGIPDAKANLVAFAKTIAADPDPEVARFGR
                     YALFDTRVSDIMNHPLNEGKPILDEIKTLIDGEQGKITADTLELARQATGHLLENNLL
                     DDAAKALEMLAAAATGSADPKVAETAAQLKDSAKLVRADLGQLLQNILTEQPDAEKKM
                     LETIKSVLAEVRPSLEVFANLEKIAQVMEVTGHAETAKAGFELIEAAYKDVSDPQVAN
                     AVTASLGGARKRLSLLGQPFAVEGVTLDGKPFDWSAYQGKVVLVDFWATWCTPCLREL
                     PNIRSNYDLFHSKGFEVVGVNLDTDAKDVKEFFALQGEFPWQTVTSQFVLEGKAVPDA
                     DGRPYSFSKLPMPEKCGVDAIPFLVLVGKDGKVDSLHVHGSKLKARLTALLGAPPTTE
                     IPADPTAPAPPAAPAAAKPPAKKTGSVQPRRLLSPVGLLVAQALLAADPPPAAVPAAE
                     DTSINPYKAKPNLTTAQLVDYIQRMLDKPKSIQSRDGFGEAVVDACDRVLKADPAAKE
                     PEQLVAIESKLGVLHREACSGKDAFDKQLVEFVGQLKDDARPRVAREVAFFNLERKAI
                     DGANVPLDQLPALLKELQEYLAKEKLTGKHLRLASTTVALVNRLEKLDEREKHFVSFG
                     GTFAKSSDKELARYGKKLAKKPAGQESDLVGKPLELTGTTAKDQAFAWEAYRGKVVLV
                     DFWATWCGPCRREMPNVKAFFEKNKDRGFEVVGVSLDQDLEALAQYIDENQLPWETLA
                     GDGTQPLAEKYGVRAIPTMMLVDKTGKIVGVAHNLGTLAPLAEKLLAGEAIAAPAK"
     gene            3471..>3913
                     /locus_tag="VFB80_06620"
     CDS             3471..>3913
                     /locus_tag="VFB80_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZN33474.1"
                     /translation="MTKPVVRYLVFDIESVADGDLVSRLRYPGENLEPAAAVQRYRDE
                     LLAKYETDFIPYTFQVPVSVVVAKVAGDFRLIDVVALDEPQFRSHVITDHFWRGWEAY
                     RRPTLVSFNGRTFDVPLLELAAFRYGLSLPGWFQTNGKSYEQPRNR"
BASE COUNT          628 a         1283 c         1270 g          732 t
ORIGIN      
        1 cggctggccc tcgaatttca gggccacgag cgggtccagc cgccgcacga catccaaggg
       61 cagcgacgtg ctgtgctgcc gcggactgtg ggcgacgaac gagacaaacc gcgcgtccga
      121 ccgcggctgc gcacgatcga gcaaggccag cagcggcacg ccgctgaacc ggcaggaaag
      181 cttcgaccag cgcgtaacgc agtgaacgtc gatcagccgt tcgacctgca agagctcgga
      241 tagctcgccc agcgagaatt ccagcggccg ggccacgagg ccggacagct cgaccttcca
      301 cgggccgcat ccgtccgccg gctgccgctc gccgacgatg ggccacttgc cgggcgcggc
      361 gagatgctgg ttgggcggca gcggcagcat acggcgctcg ggacctactt cgctggggcg
      421 gcgatggcct cgccggccag cagcttttcc gccagcgggg cgagcgtgcc aaggttgtgc
      481 gcgacgccca cgatcttgcc ggtcttatcg acgagcatca tcgtgggaat ggcccgcacg
      541 ccgtatttct cggccagggg ctgcgtgccg tcgccggcga gcgtttccca gggaagctgg
      601 ttctcgtcga tgtattgggc cagcgcttcc aggtcctgat cgaggctgac gccgacgacc
      661 tcgaagccgc ggtccttgtt cttctcgaag aacgccttca cgttgggcat ctcgcggcgg
      721 cagggcccgc accaggtcgc ccagaaatcg acgagcacga ccttgccgcg atacgcctcc
      781 caggcaaaag cctggtcctt ggccgtcgtg ccggtcagct ccagcggctt gccgacgagg
      841 tccgactctt gcccggccgg cttcttcgcg agctttttgc cgtagcgggc gagctccttg
      901 tcgctgctct tggcaaacgt gccgccgaac gagacgaagt gcttctcgcg ctcgtccagt
      961 ttttcgaggc ggttgacgag ggccaccgtg gtcgaggcca gccgcaagtg cttgcccgta
     1021 agcttctcct tggccaggta ttcctgcaat tccttgagca gcgcgggcag ctgatccagc
     1081 ggcacgttgg cgccgtcgat cgcctttcgc tccagattga aaaacgcgac ttcgcgggcg
     1141 acccgcgggc gggcgtcgtc cttcaactgg cccacgaatt ccaccagctg cttgtcgaac
     1201 gcgtccttgc cgctgcacgc ctcgcggtgc agcacgccca gcttcgactc gatcgcgacg
     1261 agctgctccg gttccttcgc cgccggatcg gccttcagca ctcggtcgca ggcatcgacc
     1321 acggcctcgc caaagccgtc gcgggactgg atcgacttcg gcttgtcgag catcctctgg
     1381 atgtaatcga ccagctgcgc ggtggtgaga ttcggcttgg ctttgtaggg gttgatgctg
     1441 gtgtcttcgg cggcgggcac ggcggccggc ggcggatcag ccgcgagcag cgcctgggcc
     1501 acgagcaaac cgaccggcga caacagccgc cggggctgca cgctgccggt ctttttggcc
     1561 ggcggctttg cggccgccgg cgctgccgga ggagctggcg cggtgggatc ggcggggatc
     1621 tcggtggtcg gcggcgcacc gagcagcgcc gtcagccggg ccttgagctt gcttccgtgg
     1681 acgtgcagcg aatcgacctt gccgtccttc cccacgagca cgagaaacgg aatcgcatcg
     1741 acgccgcatt tctcgggcat cgggagcttg gagaaggaat agggccggcc gtcggcgtcg
     1801 ggaacggcct tgccttccag gacaaactgg ctggtgacgg tctgccaggg gaattcgccc
     1861 tggagcgcga aaaactcctt gacgtccttg gcatccgtgt cgagattcac gcccacgacc
     1921 tcgaacccct tggagtgaaa cagatcgtag ttgctgcgga tgttcggcaa ttcccgcagg
     1981 cacggcgtgc accaggtcgc ccagaaatcg accagcacta ccttgccttg ataggccgac
     2041 cagtcgaacg gcttgccgtc gagcgtgacg ccttcgaccg cgaacggctg gcccagcagc
     2101 gacagccgct tgcgggcccc gccgagcgac gcggtgacgg cgttggccac ctgagggtcg
     2161 gatacgtcct tatacgctgc ttcgatcagc tcgaagccgg ccttggctgt ttcggcgtgg
     2221 ccggtcacct ccatgacctg cgcgattttc tccaggttgg cgaacacttc caggctgggt
     2281 cgcacttccg ccagcaccga cttgatcgtc tccagcattt tcttctctgc gtcgggctgc
     2341 tccgtgagga tgttctggag cagttggccc agatcggccc gcaccaattt ggcgctgtcc
     2401 ttgagctggg ccgcggtctc ggcgactttg ggatcggccg atccggtcgc ggccgcagcg
     2461 agcatctcca gggccttggc ggcgtcatcc agcagattgt tctcgagcag gtggcccgtg
     2521 gcctggcggg cgagttccag cgtgtcagcc gtgatcttgc cttgctcgcc gtcgatgagc
     2581 gtcttgatct catccaggat cggcttaccc tcgttcagcg ggtgattcat gatgtccgac
     2641 acgcgcgtgt cgaacagggc atagcggccg aagcgggcca cttccgggtc cgggtcggcg
     2701 gcaatcgtct tggcaaacgc gacgaggttc gccttggcgt cgggaattcc cacgcgggcg
     2761 aagatacggt ggatttcata catcgcctgg tagatgacgc tcttggtctt ggcatcgggc
     2821 ttgagcgtca gcgctttctc gcaggccaac agccgctgct gctggatctt caggaaattc
     2881 tggatttgct cctgctgcgt cgtccctggg ggggcttgcg actgccgggc gagcttgtcg
     2941 atctggtcca ggacttgctt tagttcgccg gtcggtgcct tgcctacggc cggcgcggtc
     3001 gcggaattgg cattcgcgct tccggtgcgc ggagaaacat tgccggcaat cggtgtggga
     3061 gagccggcgt ttgccgaagc tgagccatcc agccccccag acctgaggtc ggtgttgagt
     3121 ctggcaagct cactggcaac ggccgtatca gcggaaccgg aatcagcggg cgctgcatcc
     3181 acagggcgat acttgggcgc attggaggcg actggctgcg cctcggccga gcagccggcc
     3241 agactgagca cgccgccggc cagaatcagc actccagtcg cgctaacgct ccaaagccgc
     3301 actgagggat gcgccatggc caatcgcctt ttcaataagg gtccgcgaaa cactgcaaat
     3361 tctaagacaa accagccccc catgcatccc caaacggcgt gcaggcgaaa aacggccctc
     3421 atttcgccgt gcggaccgtt gtgttacgtt gcccgaaaag gatttccacc gtgacgaaac
     3481 ccgttgtccg ctacctggtg ttcgacatcg aaagcgtcgc cgacggggac ctggtcagcc
     3541 ggcttcgcta tccgggcgag aacctcgagc cggccgcggc cgtgcagcgt taccgcgacg
     3601 agctgctggc caaatacgaa accgacttca tcccgtacac gtttcaggtg cccgtctcgg
     3661 tcgtcgtcgc caaggtggcg ggggactttc gcctgatcga cgtggtcgcc ctcgacgaac
     3721 cgcagtttcg ctcgcacgtc atcaccgacc atttctggcg cggctgggaa gcctaccgcc
     3781 ggccgacgct cgtcagcttc aacggccgca cgttcgacgt gccgctcctg gagctggcgg
     3841 ccttccgcta cggcctctcc ctgcccggct ggtttcaaac caacggcaag tcgtacgagc
     3901 agccgcgaaa ccg
//
LOCUS       DASRJB010000242         7424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2035049, whole genome shotgun sequence.
ACCESSION   DASRJB010000242 DASRJB010000000
VERSION     DASRJB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035049"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(465..1208)
                     /locus_tag="VFB80_06625"
     CDS             complement(465..1208)
                     /locus_tag="VFB80_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZN33475.1"
                     /translation="MNRVILLAAVLSSTLAARVLAQAADPKPTIHVSGTAEVRVAPNE
                     VDLRLAIETRDVQLDEAVKQNETKTAAVLKFLKEAGIEAKDVQTDYVEIQPQYNTDRR
                     EQRIVPEYYQVRRNLGVRMRSVAKFDAVLAGALRNGANYVLGIDFRTTELRKHRDAAR
                     QQAIRAAKEKAVALAGELETKVGKATNIQEQTQSGWWGSASRYANYNAFAQNSVQVAP
                     GGGAGEESSNLSVGMISVTATVNVTFALE"
     gene            1445..3124
                     /locus_tag="VFB80_06630"
     CDS             1445..3124
                     /locus_tag="VFB80_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33476.1"
                     /translation="MTRHAPSWLLVVLLAAGSAAAQEAKSTPDVKGPPPAGPKMTAQE
                     LAEWIDERFEKEYQRLSVKPAPAVDDATYLRRLYLDLQGRVPTVAQLRDFLGDQGSFK
                     RQDYVDKLLTADARPDRYARRTSENLARVWRRMMIPASSPNAEMATQLEPWLARQFAA
                     NTPYNEFARKLMMAQPGMPARGLGGVPMPMTPADPDAEAALFQQAIGGMPENLAAAYA
                     RVFLGIRLSCAQCHDHPFTEWTQKDFWGVAAFFSGPPPMAEPGKPATEPKPPMPPPAD
                     QPLPAPTIQATGGAYENAVYTAKLLWQKEPLKELPKGQTPRQFLAEWLVGPQNPNFAA
                     TAVNRIWQYLCGRGLAGAVDELDRLTKEERGILDELSRLFVESGYDVRWLITGICKSR
                     VYQQSLTAVDADGFAQRPLKTLLPEQVFDSLEQALSLPIAKADNGPRWSGERAQFVAR
                     MNEAAPDSPIDYKGGIPQALMLMNGKITADATSLESSRTLRAVVEAPFLPPEEKIETL
                     YLAAVTRKPTAEELKYLLAHVQGQKDEAQQKQAYAQILWGLLNNPEFVLSR"
     gene            3162..4529
                     /locus_tag="VFB80_06635"
     CDS             3162..4529
                     /locus_tag="VFB80_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33477.1"
                     /translation="MKRIVQPSCVPLPNPGGDSPRFREMWSRRELLSMSLAGALGVSF
                     SGWLPRLAAAAGASGQRHKSVILLWMNGGPSQMDTFDLKPGTPNGGTFKEIETAAPGV
                     KISEHLPGIAKQMKDLAIIRSLSTTEGEHGLATQLMMTGYQSRSAVVRYPSLGSLMSK
                     ELGSDESDLPNFISLSPMRMVDAGFLGPNYSPLTVSGASDDPNTRANLSLEDLAPPTG
                     RDPKAIKDQFDILRFMQQSFGATSDAAKAHQASYNKAMRMVETQARKAFALEEEKAEL
                     RDAYGRNRFGQGCLLARRLVERGVAFVEVALAGTPGTNNWDTHADNFNAVKTLSGVLD
                     PAFASLMKDLRERGLLDSTLVVWMGEFGRTPKINPQGGRDHFPIAWSVVLGGAGIKGG
                     QAYGKTSPDGMEVTDGKVGVADLYATIFQAVGINAAKKENISPEGRPIPLVDRKAKPI
                     AELVS"
     gene            4558..6261
                     /locus_tag="VFB80_06640"
     CDS             4558..6261
                     /locus_tag="VFB80_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33478.1"
                     /translation="MCSPAVILLLALAADGPQTGVVAPGPLPAAVSGQPRPAGNAVGA
                     SDRRDVVLLLDQGPVLIRLHLALGGVSLAEARRAYVHRLVTSLDANKDGKLSRDEANR
                     SPLFRTKSRPSANAFLDSLRSQAAMTPRDVEQKVEEKIGLIMAYRDDPSSAQNDLEVF
                     KLLDADKSGVLDTAELVGAEALILVKDEDDDDCISFQEFFPPPPPPDPMAVAAGIAEE
                     PEVPQIRVSSVVRDTQDLSLPARLMRKYDKTRDLALSPAELSWPAERCKLLDADANGK
                     LDAAELASLADLPPDVELSVDLAASGLEGGLIRVEGSAGKRLDDGGRPDYAKVGFRSA
                     IMTFSHRNLDPVAASIADAMRKFNTLDTDANGYIDKDETMESVRFERGLFEMLDADGD
                     GKIFADEMKQYVTAFSEPASRNCRMHLYDTGYGFFMALDGNADGRVSRRERMNAPAAL
                     AALDRDGKLGIAEKEPVRHFHVEFVRAGFQLFAASEQLASPTPAFQQRKPTGPIWFQR
                     MDRNNDGDLVWNEFLGHIETFHALDTDGNDLLDPQEAAKYKAPSGAASRPTVDVGPAD
                     R"
     gene            6277..6693
                     /locus_tag="VFB80_06645"
     CDS             6277..6693
                     /locus_tag="VFB80_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33479.1"
                     /translation="MSTLLRCACLAFLASAPNLCYALELIEIVTKERAAELGLVVRAH
                     AAGPDAIQIELEFPAAGELKDYSSVALEIREGKKLLLSLALREEPAKPGMVAVGFAVD
                     RARLPQLTLKVVTRAQDGRIGHVLRMNEFVDLANLH"
     gene            6724..>7424
                     /locus_tag="VFB80_06650"
     CDS             6724..>7424
                     /locus_tag="VFB80_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN33480.1"
                     /translation="MADMHVGKSSPPAPQAQSADDVAVISRLREAQVRVKREMGKVVV
                     GQHDIVDSLLIGLLCRGHVLLHGVPGLGKTLMARTLARTLEMEFRRVQFTPDLMPADI
                     TGTDVMEEDQATGKHRVSFMQGPVFTNFLLADEINRTPPKTQAALLQAMQEGEVSCGR
                     ETYRLKPPFFVVATQNPIEMEGTYPLPEAQLDRFMFNVRVDYPSIDEEKRILSMTAKD
                     ESGEIHKVLSGKAIV"
BASE COUNT         1328 a         2395 c         2468 g         1233 t
ORIGIN      
        1 gcaggcggtt aagtactact tattagtgtt tgagaacccc attcgccggg ccaccctagc
       61 ggcgctcttc aacccaggaa cgacgtctga ttgcaggccc ggagggccga ccttcgttag
      121 ccctgggcgc tgcggcgcag ccgcgagccc aatgatcgtg cccgtggccg ctggtcgctg
      181 gcgggctgcc ggccagacgc tcgccgccgg gtactcagta cggcacgggg cggtcgggcg
      241 accagacatc aactatatcc acacgtggac agaattatca aatcggcccg agaattgcaa
      301 gcaacttgta cacattttgt ccatttattg ccaacttacg tcgatccacc tcggcggaac
      361 tccaagtccg gcatcgactt ggggccgagc tgcaagtcgc aacttttttt cgcgctcgcc
      421 ggcggaatgg actagtgtgg tccaatccgc gccaggccag cgggctactc cagcgcgaac
      481 gtgacgttga ccgtcgccgt gacgctgatc atgcccaccg agaggttgct gctctcttcg
      541 cccgcgccgc cgccgggcgc gacttgcacc gagttctgag cgaatgcgtt gtagttcgca
      601 tagcggctcg cggagcccca ccagccggat tgggtttgct cctggatgtt cgtcgccttg
      661 ccgaccttgg tctccaactc gccggccaga gccacggcct tttccttggc ggcgcggatg
      721 gcctgctggc gggcggcgtc gcggtgcttg cgcagctccg tcgtgcggaa gtcgatcccc
      781 agcacgtaat tcgcgccgtt gcgcagtgcg cccgccagca ccgcgtcgaa tttggcgacg
      841 ctccgcatcc gcacacccag gttgcggcgc acctggtaat actccggcac gatccgctgc
      901 tcgcggcggt cggtgttgta ttgcggctgg atctccacgt agtcggtctg cacgtccttg
      961 gcctcgatgc ccgcctcctt gaggaacttc agcaccgcgg ccgtcttggt ctcgttctgc
     1021 ttcaccgctt cgtcgagctg cacgtcccgc gtttcaatgg ccagccgcaa atccacctcg
     1081 ttgggtgcga cgcggacttc ggccgtgccg gagacgtgga tggtgggctt gggatcggcc
     1141 gcctgggcca ggacccgcgc cgccagagtg ctgctcagaa ctgccgccaa caggatgacc
     1201 cgattcataa acgactcctg ggatttgcta ggggagagtg aacctcgcgc caagaaaagc
     1261 ggattgtatc tcccgcgtat cgagcggccc gtaacgggag cgtaaaatag gccctggccg
     1321 ccaattccga gattttttgc cgcgctgctt gacgataaag acagaatgtc ttaatatcgg
     1381 ggcctgtcag cacggcagag aggctttcag gcagctagcg gtcgaaactg cgggggctta
     1441 ctccatgacg cgtcacgctc caagctggtt gctcgtggtg ctgctcgcgg ccggttccgc
     1501 ggcggctcag gaagcgaaat cgacgccgga cgtgaagggg ccgccgccgg ccgggccgaa
     1561 aatgacggcc caggaactgg ccgagtggat cgacgagcgg ttcgaaaagg aatatcagcg
     1621 gctcagcgtc aagccggcgc ccgcggtcga tgacgcgacg tatctgcggc ggctgtacct
     1681 cgatctgcag gggcgggtgc cgacggtcgc ccagctccgc gacttcctgg gggaccaggg
     1741 ttcgttcaag cggcaggact acgtcgataa gctgctcacg gccgacgccc ggcccgaccg
     1801 gtatgcccgc cgcacgtcgg agaacctcgc gcgggtctgg cggcggatga tgatcccggc
     1861 cagcagcccc aacgccgaga tggcgacgca gctggagccg tggctggccc ggcaattcgc
     1921 cgccaacact ccctacaacg aattcgcccg caagctcatg atggcccagc ccggcatgcc
     1981 cgcgcgggga cttggtgggg tgccgatgcc gatgacgccg gccgatccag atgccgaggc
     2041 ggccttgttt cagcaagcga tcggcgggat gccggagaat ctggcggcgg cctacgcccg
     2101 cgtgttcttg gggatccggc tgagctgtgc ccagtgtcac gaccatccgt tcacggaatg
     2161 gacgcagaag gatttctggg gcgtggcggc gttctttagc ggcccgccgc cgatggctga
     2221 acctggaaag cccgctaccg agcccaagcc gccgatgccg ccgccggccg atcagccgct
     2281 gccagcgccg acgatccagg cgacgggcgg ggcctacgag aatgccgtct atacggccaa
     2341 gctgctgtgg cagaaagagc cgctcaagga gctgcccaag gggcagacgc cgcggcagtt
     2401 cctggccgag tggctggtcg gaccgcagaa cccgaatttc gcggcgacgg ccgtcaaccg
     2461 catctggcaa tatctgtgcg gccgcggcct ggccggcgcc gtcgatgagc tggaccgcct
     2521 gacgaaggaa gagcggggaa tcctcgatga gctgtccagg ttgttcgtgg agtcgggcta
     2581 cgacgtccgc tggctgatca cgggcatctg caagagccgc gtctatcagc aatcgctgac
     2641 ggcggtcgat gccgacggct tcgcccagcg cccgctgaag acgctgctgc cggagcaggt
     2701 gttcgattcg ctcgagcagg cgctgagcct gccgatcgcc aaggccgaca acggcccccg
     2761 gtggagcggc gagcgggcgc agttcgtggc ccgcatgaac gaagcggccc cagacagccc
     2821 gatcgactac aaggggggca ttccgcaggc cctgatgctg atgaacggca agatcaccgc
     2881 cgacgccacc agcctggaga gcagccgcac gctgcgggcg gtggtcgaag ccccgttttt
     2941 gccgccggag gagaagatcg agacgttata tctggcggcg gtgacgcgaa agccgaccgc
     3001 ggaggagctg aaatacctgc tggcccacgt gcagggccag aaagatgaag cgcagcaaaa
     3061 gcaagcctat gcccagattc tgtggggcct gctcaataac cccgagttcg tcttgagtcg
     3121 ctaaccgcac gttcatcgtc tgacagatag cgaggacagc catgaagcgc attgtgcagc
     3181 cgagttgtgt gccgcttccc aaccccggcg gcgattcccc caggttccgc gagatgtggt
     3241 cgcggcgcga gctcctgagc atgagcctgg ccggggcgct gggcgtgtcg ttcagcggct
     3301 ggctgccgcg cctggcggcg gcggccggcg ccagcggcca gcggcacaag tcggtcatcc
     3361 tgctgtggat gaacggcggc ccgagccaga tggacacgtt cgacctcaag ccgggcacac
     3421 ccaacggagg cacgttcaag gaaatcgaaa ccgccgcccc gggcgtgaag atcagcgagc
     3481 atttgccggg catcgccaag cagatgaaag acctggcgat catccgcagt ctttcgacga
     3541 ccgaaggcga acacggcctg gcgacgcagc tgatgatgac gggctatcag tcgcggagcg
     3601 cggtggtgcg ttatccgtcg ctcgggtcgc tgatgtcgaa ggaactgggc agcgacgaga
     3661 gcgacctgcc gaacttcatc agcctctcgc cgatgcgaat ggtcgatgcg ggctttttgg
     3721 gtccgaatta ttccccgctg acggtgagcg gggcgagcga cgacccgaat acgcgggcca
     3781 acttgtcgct cgaggacctc gcgccgccga ccggccgtga tcccaaggcc atcaaagatc
     3841 agttcgacat cctgcggttc atgcagcagt cgttcggtgc gaccagcgac gccgccaagg
     3901 cccaccaggc cagctacaac aaggcgatgc ggatggtcga gacgcaggcc cgcaaggcgt
     3961 ttgcgctgga agaggagaaa gccgagctcc gcgacgccta tggccgcaac cgctttggcc
     4021 aagggtgcct cttggcgcgg cggctggtcg agcgtggcgt ggcgttcgtc gaggtcgccc
     4081 tggccggcac gccgggcacc aacaactggg atacgcacgc cgacaacttc aacgcggtca
     4141 agacgctttc gggcgtgctc gatcccgcgt tcgcctcgct catgaaggac ctccgcgagc
     4201 ggggcctctt ggactcgacg ctggtcgtct ggatgggcga gttcggccgc acgccgaaga
     4261 tcaacccgca gggaggccgc gaccacttcc ccatcgcctg gagcgtcgtc ctgggcggcg
     4321 cggggatcaa ggggggccag gcctatggca agaccagccc cgacggcatg gaagtgaccg
     4381 acggcaaagt cggcgtggcg gacctctatg ccacgatctt ccaggcggtt ggaatcaacg
     4441 cagccaagaa ggagaacatc tctcccgaag gccgcccgat tcctctggtc gatcgcaaag
     4501 cgaagccgat cgcggagctc gtgagttagt tgcctcgggg acagagggct tgggtgcatg
     4561 tgctctcccg ccgtaatcct gctgctcgcg ctcgcggccg acggcccgca aactggcgtc
     4621 gtggccccgg gcccgctgcc ggccgccgtg agcggtcagc cgcggcccgc cgggaatgcc
     4681 gtgggagcca gcgaccggcg agatgtcgtg ctcctcttgg accagggacc ggtgctgatc
     4741 cgcttgcacc tggcgctcgg cggcgtttct ttggccgagg cccgccgggc ttacgtgcac
     4801 cgcctggtca cctcgctcga cgccaacaag gacggcaagc tgtcgcgcga cgaggccaac
     4861 cgctcgccgc tgttccgcac caagagccgg ccgagtgcga atgcgtttct cgacagcctc
     4921 cggtcgcagg cggcaatgac gccccgcgac gtggagcaga aagtcgaaga gaaaatcggc
     4981 ctgatcatgg cctatcgcga cgatccgtcg tcggcgcaga acgacctgga ggtgttcaag
     5041 ctgctggacg ccgacaagtc cggcgtgctc gacacggcgg agctggtcgg cgcggaggcc
     5101 ttgatcctgg tcaaggacga ggacgacgac gactgcatca gcttccagga attctttccg
     5161 ccgcctccgc cgcccgatcc catggccgtc gctgccggga tcgccgagga gccggaggtt
     5221 ccgcagatca gagtgtccag cgtggttcgc gacacccagg atctgtcgct gcccgcgcgg
     5281 ctgatgagga aatacgataa gacccgcgac ctcgcgctca gcccggcgga gctgtcgtgg
     5341 ccggccgagc gctgcaagtt gctggatgcc gacgcgaacg ggaagctcga tgcggctgaa
     5401 ttggcgtcac tggcggattt accgccggac gtggaattga gcgtcgatct ggcggcgtca
     5461 ggattggaag gcgggttgat ccgtgtcgaa ggctccgccg gcaagcggct ggacgacggc
     5521 gggcggcccg actatgcgaa ggtcggcttc cgctccgcga tcatgacgtt ctcgcaccgc
     5581 aatctcgacc cggtagccgc gtcgatcgcc gacgccatgc ggaaattcaa cacgctcgac
     5641 accgacgcca acggctacat cgacaaggac gagacgatgg agagcgtccg cttcgagcgc
     5701 gggctgttcg aaatgctcga cgcggacggc gacggcaaga tctttgccga cgagatgaag
     5761 caatatgtca ccgcgttcag cgagccggcg tcgaggaatt gccggatgca cctgtacgac
     5821 acgggctacg ggttcttcat ggccctggac ggcaacgccg acggccgcgt ttcccgccgc
     5881 gagcggatga acgcgccggc ggccctggcg gccctggacc gcgacggcaa gctgggcatc
     5941 gccgaaaagg agccggtgcg gcattttcac gtcgagttcg tccgcgccgg ctttcagctt
     6001 ttcgcggcca gcgaacagct ggcctcgccg acgcccgcgt ttcagcagcg gaagccgacg
     6061 gggccgatct ggttccagcg gatggaccgc aacaacgacg gcgatttagt gtggaacgag
     6121 ttcctcggcc acatcgagac tttccacgcg ctggacaccg atggcaacga cctgctcgat
     6181 ccgcaggaag cggccaagta taaggcgccc tcaggcgcag ccagccgccc aacggttgac
     6241 gtgggaccgg cggatcgctg aacggagagt aagatcatgt cgacgctgct ccgctgcgcc
     6301 tgcctggcgt tcctggcatc cgctcccaac ctctgttacg ccctcgagct aatcgaaatc
     6361 gtgaccaaag agcgggccgc cgagctgggg ctggtggtcc gggcccacgc cgccggcccc
     6421 gatgccatcc agattgagct cgagttccca gccgcgggcg aactgaagga ctacagcagc
     6481 gtggccttgg aaatccgcga aggaaagaag ctgctgctct cgctggcctt gcgggaggag
     6541 ccggccaagc cgggtatggt tgccgtcggt tttgccgtgg accgagccag gctgcctcag
     6601 ctgaccctga aagtggttac gcgggcgcag gacggcagga tcgggcacgt gctccggatg
     6661 aacgaatttg tcgatctcgc taacctccac tgacgcctcc tcctctgcct tccctggtgg
     6721 ttcatggccg atatgcacgt tggaaaatcg tcgccgcccg cgcctcaggc ccaatcggcc
     6781 gacgacgtcg ccgtcatcag ccggctgcgc gaggcccagg ttcgcgtgaa gcgcgagatg
     6841 ggcaaggtcg tcgtggggca gcacgacatc gtcgattcgc tgctgattgg cctcttgtgc
     6901 cgcgggcacg tgctgttgca cggcgtgccg gggctgggca agacgttgat ggctcgcacg
     6961 ctggcccgca cgctcgagat ggagtttcgc cgtgtgcagt tcacgcctga cctgatgccc
     7021 gccgacatca cgggcaccga cgtgatggaa gaggaccagg cgaccggcaa gcaccgcgtc
     7081 tcgttcatgc aagggccggt gttcaccaac ttcctgttgg ccgacgaaat caaccgcacg
     7141 ccccccaaaa cccaggccgc cctgctccag gccatgcagg aaggcgaagt ctcctgcggt
     7201 cgcgagacct atcggctcaa gccgccgttc tttgtcgtcg ccacgcagaa cccgatcgag
     7261 atggaaggca cctacccgct gcccgaagcc cagctcgacc gcttcatgtt caacgtccgg
     7321 gtcgattacc cgagcatcga cgaggagaag cgcattctgt ccatgaccgc gaaggacgag
     7381 tcgggcgaga ttcacaaggt gctgagcggc aaggccatcg tcaa
//
LOCUS       DASRJB010000243         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_69929, whole genome shotgun sequence.
ACCESSION   DASRJB010000243 DASRJB010000000
VERSION     DASRJB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69929"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFB80_06655"
     CDS             <1..278
                     /locus_tag="VFB80_06655"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZN33481.1"
                     /translation="TDGRFSGGSHGFLVGHVTPEAQLGGPIALVQNGDRLTIDAEANR
                     IDIALSDAELARRRAAFKSPPFKSTRGTLYKYIKNVKTASEGCVTDE"
     gene            complement(298..897)
                     /locus_tag="VFB80_06660"
     CDS             complement(298..897)
                     /locus_tag="VFB80_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33482.1"
                     /translation="MSPLDIRPPNEALAPKVEPLWIAVALWPFRVPIVTGLAATVLFV
                     LAMFCAMIAGPLQSPVAWGCSVLFGLLAATAAAALVLRRLRSSEVAAWGMQHYRQTLT
                     GSTAFIIALFCAIYGWFLTILIHSNVFAANRVGGTKYWATVAIAGGASLYLTIYGRRR
                     LFQTLAKPETSRMTLTAGQALLTIGLCVAAGVYFAWLMV"
     gene            complement(1460..1942)
                     /locus_tag="VFB80_06665"
     CDS             complement(1460..1942)
                     /locus_tag="VFB80_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HZN33483.1"
                     /translation="MRIGVISDTHAHVQNTQAAVRMLESLDVEAVLHCGDICSAEIPL
                     LLKTWPAHFVFGNCDHDLDSLRQAIEQAGLSCYGQFGDFTLAGRRIALLHSHDVRLFR
                     RVTTCGDYDLVCYGHTHVAEQHRAGRTLVLNPGALYRANPHSIAIVELETLTATIVPL
                     "
     gene            2184..3026
                     /locus_tag="VFB80_06670"
     CDS             2184..3026
                     /locus_tag="VFB80_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33484.1"
                     /translation="MSHLIAEVWMHARDTAELAAWIAVHSPVLVQSGGRAPQAASEQY
                     WSASKCRLERWRRLLTELTAAADQPHKPATLAWPRVRPVLEEILTSELLTRIWTAAAV
                     AYDAARRDQDLEPIARNIYSGHLDVRRRLLGLMTDARVFQEQEVKALDLLRRRVERWG
                     DMLLAHLADSVPIDEFAFDAERARDFASDLDRDAARNDRDFTTQLMFSSLRASFSFDL
                     ADRTPNGDLSRKIGSAILACFGADLPVSTGLSPALWLERISRTASDTEGMIEELLRLD
                     RPVA"
     gene            complement(3089..>4445)
                     /locus_tag="VFB80_06675"
     CDS             complement(3089..>4445)
                     /locus_tag="VFB80_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN33485.1"
                     /translation="SCNPKLLIADEPSTALDVTIQAQILDLLRQLRDQRGMSVLFITH
                     DLGVIAEIADEVAVMFRGKLVEYGKVLNLFENPQHPYTKGLLACKPRLTTPYKVLPTV
                     SDFMEASGQGDTLKIVAKTMTAERLEQLTTQGRGRLLHPKSRLKAMLHPWEQGHHAKD
                     TTTVPEGQQPLLSVKDLKVHFPIRKGVFLRTAGHVKAVDGISFDVYRGQTLGLVGESG
                     CGKTTAGRAILRLIEPDAGVVRYDGVDLAQLGGSEMRKLRRKLQIIFQDPYGSMNPRM
                     TIESALTEPMVIQGIGKGGQDRRDRAVALLEEVGLEAAHLRRYPHEFSGGQRQRICIA
                     RALTVEPEFIVCDESVSALDVSVQAQVLNLLKRLQESHGLTYVFISHDLSVVKFMADM
                     MAVMQEGKIVEFGPSESIYADPQMAYTKELISAAPKDDLDSIRRRQQAREAALARRRS
                     S"
BASE COUNT          791 a         1433 c         1432 g          789 t
ORIGIN      
        1 tcaccgatgg ccgcttctcc ggcggctccc acggattcct cgtcggccat gtcacccccg
       61 aagcccaact cggcggcccc atcgccctcg ttcaaaatgg cgaccgcctc accatcgacg
      121 ccgaagccaa ccgcatcgac atcgccctct ccgatgccga gctcgcccgc cgccgcgccg
      181 ccttcaagtc cccacccttc aaatccaccc gcggcaccct ctacaagtac atcaagaacg
      241 tgaaaaccgc gagcgagggg tgtgtgacgg atgagtgagg tcaccacact tcattgctca
      301 caccatcagc cacgcgaaat agacgcccgc tgcgacgcaa agaccaatcg taagcagagc
      361 ttggccggcg gtgagtgtca tgcgggaggt ttcgggcttg gcgagggtct ggaagagcct
      421 gcggcggccg tagattgtga gatacaggga ggcaccgccg gcaatggcga ccgtcgccca
      481 gtatttagtg ccgcctactc gattggctgc gaatacgttg ctgtgaatca agattgtaag
      541 gaaccagccg tagatggcgc agaagagagc aatgatgaag gcggtcgatc cggttagtgt
      601 ctggcgatag tgctgcattc cccaggccgc gacctcactg gatcgaagtc gccgcaacac
      661 cagcgccgcg gcagccgtcg ccgcgagcag gccaaacaag accgaacagc cccaggccac
      721 cggcgactga agtggcccgg caatcatggc gcaaaacatc gccaggacga acagtactgt
      781 cgctgcgagg cccgtgacaa tcggtacgcg aaatggccac agcgcgaccg cgatccacag
      841 cggttcgacc ttcggcgcaa gggcttcgtt gggagggcga atgtcgaggg gcgacatacg
      901 ggacacttta gcgacgagcg tcggccttcg cccccctacc ttcgggtgcc ctcggaatcg
      961 gccaaccaca acctgtagtg ggtcggcccc gagcgaacac aatctgttgt ggttgacttg
     1021 acagatggtg gacggatcat tacaattcca accggcggcg gtttcccagc ggccgtggct
     1081 gaattcacga gattccggcc ttttgatggt ccgcgtcggc gtgggtcttt tggaattcgt
     1141 gaaggtgtca atgtccgtcg tgcgactccg tcgtacgaag cagcgctggg ggcggcgaat
     1201 tcttcaccat ggtgaaaaat ctaagttgcg agcgggccgg agtttagggc gagcggagcg
     1261 aattattcac cagatccgca ggtatcgcag tcgcatggtg aattaatcgg cgactgttcg
     1321 gcggagggga aagttggaag tacaggccgc agcgggcttt gcggaacgag cggccagggc
     1381 ccggggccga ttaattcacc acagagcgcg ccgacccggt gataaacgaa actgtcgccg
     1441 ccagcgcgcc gcggcctggc tacagcggaa caatggtcgc cgtcagggtt tccaattcca
     1501 cgatggcgat tgaatgcggg ttggcgcggt acagcgcgcc cgggttgagc acgagcgtgc
     1561 ggcccgctcg gtgttgctcg gccacgtgcg tatggccgta acagacgagg tcgtagtcgc
     1621 cgcacgtggt gactcggcgg aacagtcgga cgtcgtggct gtgaagcagc gcgattcgcc
     1681 ggccggccag cgtgaaatcg ccgaactgtc cgtagcacga caggccggcc tgctcaatcg
     1741 cctgccgcag cgagtccagg tcgtggtcgc agttgccgaa gacgaaatgc gccggccacg
     1801 tcttgagcag cagcgggatc tcggcggagc aaatgtcgcc gcagtgcagc acggcttcga
     1861 cgtcgagcga ctcgagcatc cgcacagccg cctgcgtgtt ttgcacgtgc gcgtgggtgt
     1921 cgctgatcac gccgattcgc atagcgctgg cccaaaacgg tgttgctcaa atgccacgcc
     1981 gttgtccggt tcgcgccgcc gaaagtcaac cccattggaa cggcccagcg accagcgccc
     2041 tcggcgcaag tctgggtccc atagtgactt gcaacaacct ggttcgcgct gccggtgggc
     2101 ggcattagcc ttgcgatact cattgtcgcc gccgacggct tccggcccac gtcccctcgg
     2161 gcctggagcg cgtcggcggc tttttgagcc atttgatcgc cgaggtctgg atgcacgccc
     2221 gcgacaccgc agagctcgcc gcctggattg ccgttcattc tccggtgctg gtgcagtcgg
     2281 gcgggcgggc gccgcaggcg gccagcgagc aatactggtc ggcgtccaag tgccggctgg
     2341 agcgctggcg gcgattgctg acagaactca ccgcggcggc cgatcagccg cacaaaccgg
     2401 cgacgctcgc ctggccgcgc gtgcgccccg tgctggaaga aatcctgacc agcgagctcc
     2461 tcacgcgaat ctggaccgcc gccgccgtcg cctacgacgc cgcccgccgc gatcaggacc
     2521 tggagccgat cgctcgcaac atctacagcg gccacctcga cgtgcgccgc cggctgctgg
     2581 ggctgatgac cgacgcccgc gtattccagg aacaggaagt caaagccctc gatctgctcc
     2641 ggcgccgcgt cgagcggtgg ggcgacatgc tgctggccca cctcgccgat tcagtgccga
     2701 tcgacgagtt tgccttcgac gccgagcggg cccgcgattt tgcttccgac ctcgatcggg
     2761 acgccgcccg taacgaccgc gatttcacga cgcagctcat gttctcttcg ctgcgggcct
     2821 cgttttcgtt cgacctcgcc gaccgcactc ctaacggcga cctgagccgc aagatcggct
     2881 cggcgatcct cgcctgcttc ggcgccgacc tgccggtctc caccgggctc tcaccggcgc
     2941 tatggctcga gcgaatcagc cgcaccgcca gcgacaccga aggaatgatc gaagagctgc
     3001 tgcggctgga tcgccccgta gcataagcgt cctcgcttgt gcagcgatta gaagaggatg
     3061 gaacggtgag caagcgagga cgcttgctct acgacgagcg cctccgcgcc agggcggctt
     3121 cgcgagcttg ctgccggcgg cggatgctgt ccaggtcgtc cttgggggcg gcgctgatca
     3181 gctccttggt gtaggccatc tgcgggtcgg catagatgct ctcggacggc ccaaactcga
     3241 cgatcttgcc ttcctgcatg acggccatca tatcggccat gaacttcacg acggagaggt
     3301 cgtggctgat gaagacgtag gtcagaccgt gcgactcctg aagccgcttg agcaggttaa
     3361 gcacctgcgc ctgcaccgag acgtcgaggg ccgagaccga ttcgtcgcag acgatgaact
     3421 ccggctcgac ggtcagcgcc cgcgcgatgc agattcgctg ccgctggccg cccgagaatt
     3481 cgtgaggata ccgccgcagg tgcgcggcct ccaggccgac ttcttcgagc agcgccacgg
     3541 cgcggtcgcg gcggtcttga ccgcccttgc cgatgccctg aatgaccatc ggctcggtga
     3601 gggccgattc gatcgtcatc cgcgggttca tgctcccgta ggggtcctgg aagataattt
     3661 gcagcttgcg gcgcaacttg cgcatctcgc tgccgccgag ttgtgccagg tcgacgccgt
     3721 cgtagcggac gacgcccgcg tcgggttcga tcagccgcag gatggcccgg ccggccgtcg
     3781 tcttgccgca gcccgattcg ccgaccaggc ccagcgtctg cccgcgatag acgtcaaagc
     3841 tgatcccatc gaccgccttc acgtggccgg cggtccgcag gaagaccccc ttgcgaatcg
     3901 ggaagtgtac cttgaggtct ttaaccgaca agagaggctg ctggccctcg ggcaccgtcg
     3961 tcgtgtcctt ggcgtggtgg ccttgctccc aaggatgcag catcgccttg agccgcgact
     4021 tgggatgcag caggcggccg cggccttgag tggtgagctg ctccaaccgc tcggccgtca
     4081 ttgtcttggc gacgatcttc agggtatcgc cttgcccgct ggcctccatg aagtccgaga
     4141 ccgttggcag cactttgtag ggcgtggtca gccgcggctt gcaggccagc aggcccttgg
     4201 tgtaaggatg ctgcgggttc tcgaagaggt tgagcacttt gccgtactcg accagcttcc
     4261 cgcgaaacat cacggcgacc tcgtcggcga tctcggcgat cacgcccaag tcgtgcgtga
     4321 tgaacagcac gctcatgccg cgctggtctc gaagctggcg gaggaggtcg agaatctggg
     4381 cctgaatcgt cacgtcgagg gccgtgctcg gctcgtctgc gatcagcagc ttcgggttgc
     4441 acgag
//
LOCUS       DASRJB010000244         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1480164, whole genome shotgun sequence.
ACCESSION   DASRJB010000244 DASRJB010000000
VERSION     DASRJB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480164"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="VFB80_06680"
     CDS             complement(<1..2027)
                     /locus_tag="VFB80_06680"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZN33486.1"
                     /translation="MTAIRPQPEDRLARLLATTETDAAPSDRAALAALRDRAAAEFAA
                     AGQEPTLTHEQRSTMFFMRALTVLAATAAAVLVAFFLREANRPAAELTLGEVHQKLLA
                     ADSYRAEWELRDQRRVIWGSGDALRFDGESPTQYAIARGDKFWEINEADNRATPSPSR
                     YYSPQLAAADPFRILDLPTAEVAGVKPQARRNEDGRELLVYFCSLAQPDRPEYEVAVD
                     AATGLPDSVRLLRSGGKPVSDVALKFVAFNQAPADPMKFQVPATLTEDGRIGKLMDHQ
                     GLVSLRPLTATRWTPICGETVLRPGDWLRTDFQGANAATVQLAPQTKLILGPGTLVEL
                     TKPDQIRIHSGIVEVTASPKSENFSGVVKLSGPAGASLDVGGVLLLRADERGLTRVAK
                     DPPWLAGYKGTTAKESLGSLITMIDGREVPLTVGYHNVSVEIRDQIARTTIEESFVNH
                     TDARLEGQFHFPLPADASISGFGMWIGSKLVEADIVEKQRAREIYETILREKRDPGLL
                     EWTGGNIFKARVFPIEPKSEKRIKIVYTQVLPRKGDSYRYSYALQSELLRQNPLRKLD
                     LGVTVASTQPLAKVESPTHQARGQATEHSARLEFSAQKYTPARDFEVVVTLAGPSQDV
                     TFIPHQRGEDGYFLLMLQPPAEGGVWQRDVVPDGSPLNVIVLADTSASMDRS"
     gene            complement(2024..>2290)
                     /locus_tag="VFB80_06685"
     CDS             complement(2024..>2290)
                     /locus_tag="VFB80_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN33487.1"
                     /translation="DRALDPAELLESAETAAAVREALRALPVDYELLLVGKYLDGLSL
                     EELAEAEESTADAISSKLARARRAFRESFAQLAGSQDSTPVRLP"
BASE COUNT          335 a          733 c          821 g          401 t
ORIGIN      
        1 gagcggtcca tcgacgcgct ggtgtcggcc agcacgatca cgttcagcgg cgagccgtcg
       61 ggcacgacgt cgcgctgcca gacgcccccc tcggccggcg gctgcagcat cagcaggaaa
      121 tagccgtcct cgccccgctg gtgcggaatg aaggtcacgt cctgcgacgg gccggcgagc
      181 gtcaccacga cctcgaagtc gcgcgccggc gtgtatttct gggccgagaa ctccagccgg
      241 gccgaatgtt cggtcgcctg cccgcgggcc tggtgcgtcg gcgactcgac cttggccagc
      301 ggctgcgtgc tggcgaccgt cacgcccaag tcgagcttcc gcagcgggtt ctgccgcaag
      361 agctcgctct gcagggcgta gctgtagcga tagctgtcgc ccttccgcgg caagacttgc
      421 gtgtaaacga tcttgatccg cttctcgctc ttgggctcga tcggaaagac gcgggccttg
      481 aagatgttgc cgccggtcca ttcgaggagg ccggggtcgc gtttctcgcg gaggatcgtt
      541 tcgtagattt cgcgggcccg ctgcttctcg acgatgtcgg cctcgaccaa cttgcttccg
      601 atccacatgc cgaagccgct gatcgaggcg tcggcgggca gcgggaagtg gaactgccct
      661 tccaggcggg cgtcggtgtg gttgacgaac gattcctcga tcgtggtgcg ggcgatctgg
      721 tcgcggattt cgaccgagac gttgtgatag ccgaccgtga gcggcacttc gcgaccgtcg
      781 atcatcgtga tcagcgagcc aagcgactct ttggccgtgg ttcccttgta gccggcgagc
      841 cagggcggat ctttggccac gcgcgtcagc ccgcgctcgt cggcccgcag caagagcacc
      901 ccgccgacat ccagcgacgc accggccgga ccgctcagct tgacaacgcc gctgaagttc
      961 tcgctcttgg ggctggcagt gacttcgacg atgccggagt ggatgcggat ctggtctggt
     1021 ttggtcagct cgacgagcgt tccgggcccg agaatcagtt tcgtctgcgg cgcgagctgc
     1081 accgtggcgg cattggcgcc ctgaaagtcc gtccgcagcc agtcgcccgg ccgcaaaact
     1141 gtctcgccgc agatcggcgt ccagcgcgtt gccgtgagcg gccgcagcga cacgagtccc
     1201 tgatggtcca tcagtttgcc gatccggccg tcttcggtca gcgtggcggg gacctggaac
     1261 ttcatgggat cggccggggc ttgattgaag gccacgaact tgagcgcgac gtcgctcacc
     1321 ggcttgccac ccgagcggag aagccgcacc gagtcgggca gtccggtcgc cgcatcgacg
     1381 gcgacttcgt attcgggccg gtcgggctgg gcgagggagc agaagtaaac gagtagttcg
     1441 cggccgtctt cgttgcgcct ggcctgcggc ttgacgccgg ccacttcggc ggtgggcagg
     1501 tctaggatgc ggaacgggtc tgcggcagcc agctgcggcg agtaataccg cgagggggag
     1561 ggcgtggctc ggttatccgc ctcattgatt tcccagaact tatcgccgcg ggcgatcgca
     1621 tactgcgtgg gcgattcgcc gtcgaatcgc agggcgtcgc cgctgcccca gatcacgcgc
     1681 cgctggtcgc gcagctccca ctcggcccgg tacgagtcgg ccgcgagcag cttctgatgg
     1741 acttcgccca gcgtcagctc cgcggcgggg cgattggctt cccttaggaa gaatgccacc
     1801 agcacggcgg cggcggtagc ggccaggacg gtcagcgcgc gcatgaagaa catcgtggat
     1861 ctctgctcgt gggtgagagt cggctcctgg ccggcggcgg cgaattcggc ggctgcgcgg
     1921 tcgcggagcg cggccagggc ggcgcggtcg ctcggcgcgg cgtcggtttc ggtcgtggcc
     1981 agcagccggg cgaggcggtc ttcgggttgg ggtcgaatgg cggtcatggc aaacggacag
     2041 gcgtggagtc ctgggatccg gcgagttggg cgaacgattc acgaaaggcc cggcgggcgc
     2101 gggccagctt cgagctgatc gcgtcggcgg tgctctcctc ggcttcggcc agctcctcca
     2161 gcgacaggcc atcgaggtat ttgccgacca gcagcagctc gtaatcgacc ggcagggcgc
     2221 ggagcgcctc gcggacggcg gccgcggtct cggccgactc cagcaattcc gccgggtcaa
     2281 gcgcgcggtc
//
LOCUS       DASRJB010000245         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2325189, whole genome shotgun sequence.
ACCESSION   DASRJB010000245 DASRJB010000000
VERSION     DASRJB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325189"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VFB80_06690"
     CDS             complement(<1..1901)
                     /locus_tag="VFB80_06690"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZN33488.1"
                     /translation="MSRLGFEPILDSYLAVALFALVLVLLLGVTPQFGSLTRSRRITL
                     VALRALVVLLAVLAMLRPTWITTIHEPRTSVLLLLLDASRSMILPADGGSRTRWEAQS
                     QAFAAARDELARLAGKTEVRVFAYDNRLLPLEFSGGQIKLPQDPAGQQTDIGTTLHEA
                     LRGEQGKRVAAVLLSGDGVQTAFEPLIETQEAARKLRDDFAAPLYTVTFGLSGNAAQA
                     RDLAIERLDEQFTVFVKNEVVIRGLVRVRGYVKQDLPIELLLVDEKNQPQTIGRRTIQ
                     AAEDNQQVEVEFTYTPQTPGHYRLTLRADKQPGELVEKNNKLDAYLTVLEGGLRVLYL
                     DGESRFERKFLRWALDASPDIELDDAVIPRRSQVAESGQPGWPVDLGPDFAAGKYDAY
                     ILGDLDAAALGQANLEKLAQEVGQRGKGLLMIGGRSSFGRGHYFDTPLRDALPIVIDR
                     IEGAEFGDAALERFFLPGPIQMVPAGPHAITRLTGDADNAAAWSKLPPLAWANKFAGV
                     KQVPGVRILLQSPQGQPLLVSGEFGAGRTLAFAGESTYLWRMHDFQKEHNRFWRQVIL
                     WLVKRDDLNRDEVWIRLDQRRFNPGSKVQIQAGARTASGDPIGDARLETFLVHPGGKR
                     EPLVLSQAD"
     gene            2046..>2822
                     /locus_tag="VFB80_06695"
     CDS             2046..>2822
                     /locus_tag="VFB80_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33489.1"
                     /translation="MKTPRNFSRRKFLATTTAAMATVTILPSGLLKAAPNDKPNIAAV
                     GVGGKGWGDITETSKDGAAQVVAFCDVETGGGRGRKANSGFGAAAEKWPDANRYTDWR
                     EMLEKEGKRLNGITVSTPDHMHAPITMTAIQMGLATYTQKPLTRTIHEARQLMLAARK
                     AKVVTQMGNQGHSGYGYRALVALVQGGAIGRIKEAHAWSNRPIWPQGIDRPPGSSPVP
                     QTLNWDLWLGVAPERPYVDKAYAPFNWRGWYDFGAGALGDM"
BASE COUNT          453 a          941 c          992 g          436 t
ORIGIN      
        1 tggtcggcct gcgacaacac cagcggctcg cgcttgccgc cgggatgcac caggaacgtc
       61 tccagccggg cgtcgccgat cggatcgccc gacgcggtcc gcgcgccggc ctgaatctgc
      121 accttgctgc cggggttgaa ccgccgctgg tcgaggcgga tccagacttc gtcccggttc
      181 aggtcgtcgc gcttgaccag ccacaagatg acctgccgcc agaagcggtt gtgctctttc
      241 tggaagtcgt gcattcgcca caggtaggtc gattcgcccg cgaaggccag cgtgcggccc
      301 gcgccgaatt cgccgctgac cagcagcggc tgcccctgcg gcgactgcag cagaatgcgg
      361 actccgggaa cctgcttcac gcccgcgaac ttgttggccc aggccagcgg cggcagcttg
      421 ctccaggccg ccgcgttgtc ggcgtcgccg gtcagccgcg tgatcgcgtg tggcccggcc
      481 ggaaccatct ggatcgggcc cgggagaaag aaccgttcga gcgccgcgtc gccgaactcg
      541 gccccttcga tccggtcgat gacgatcggc agggcgtcgc gcagcggcgt gtcgaagtag
      601 tgcccgcggc cgaagctcga gcggccgccg atcatcaaca ggcccttgcc gcgctggccg
      661 acttcctggg ccagcttctc gaggttcgcc tggcccagcg ccgccgcgtc gaggtcgccc
      721 aggatgtacg cgtcgtattt gcccgcggcg aagtccggac ctagatcgac cggccagccg
      781 ggttggccgg actccgctac ctgagagcgc cgcgggatca cggcgtcgtc gagttcgatg
      841 tcgggcgagg cgtcgagcgc ccagcggagg aacttgcgct cgaaccggga ttcgccatcc
      901 agatagagca cccgcagacc gccctcaagc acggtgaggt aggcgtcgag cttgttgttc
      961 ttctcgacca gctcgcccgg ctgcttgtcg gcccgcagcg tcagccggta atgccccggc
     1021 gtctgcggcg tgtaggtgaa ttcgacctcg acctgctggt tgtcctcggc ggcctggatc
     1081 gtgcggcggc cgatcgtctg cggctggttc ttctcgtcca cgagcaagag ctcgatgggc
     1141 aggtcctgtt tcacgtagcc gcggacgcgc accaggccgc ggatgacgac ctcattcttc
     1201 acgaagacgg tgaactgctc gtcgagccgc tcgatggcca ggtcgcgggc ctgggcagcg
     1261 ttcccggaga ggccgaaggt caccgtatag agcggcgcgg cgaagtcgtc gcggagcttg
     1321 cgggcggcct cttgcgtttc gatgagcggc tcgaaggcgg tctgcacgcc gtcgcccgag
     1381 agcagaaccg cggccacgcg cttgccttgc tcgccgcgga gggcctcgtg cagtgtcgtg
     1441 ccgatgtcgg tctgctgccc cgccgggtcc tggggcagct tgatttggcc gcccgagaac
     1501 tccagcggca gcaggcggtt gtcgtaggcg aagacgcgga cttccgtctt gccggccagg
     1561 cgggccagct cgtcccgggc cgcggcgaag gcctgcgact gcgcctccca ccgcgtgcgc
     1621 gagccgccgt cggccggcag gatcatgctg cggctggcgt cgagcaggag cagcagcacg
     1681 ctggtccgcg gctcgtggat cgtcgtgatc caggtcggcc gcagcatggc cagcacggcc
     1741 agcagcacga ccagggcccg cagtgcgaca agcgtgatcc ggcgggagcg ggtgagcgac
     1801 ccgaactgcg gcgtcacgcc gagcagcagc accagaacga gcgcgaagag cgccacggcc
     1861 aggtagctgt cgagaatggg ctcgaagccg agacgggaca tttcgggggt cgggagtcgg
     1921 gggacgggag tcggcgacta cttcaatgta cctgcgcggg agtggttttg gttcgttgat
     1981 ccgggcgcgg cgcagcttta cactcagtcg cagacaacgc cgatctctcc ctttcaggtg
     2041 tcaacatgaa aacgccgcga aacttctctc gccgcaagtt cctggccacg acgacagccg
     2101 cgatggccac ggtcacaatt ctgccgtctg ggttgcttaa ggccgcgccg aacgacaagc
     2161 cgaacattgc ggcggtgggc gtgggtggca agggctgggg ggacatcacg gaaacatcga
     2221 aagacggcgc agcgcaggtt gtggcgttct gcgacgtcga gaccggcggc ggccgcggcc
     2281 ggaaggccaa ctcgggcttc ggggccgcgg ccgagaagtg gcccgacgcc aaccgctaca
     2341 ccgactggcg cgaaatgctc gaaaaggaag gcaagcggct gaacggcatc accgtctcga
     2401 cgcccgacca catgcacgcg ccgatcacca tgacggcgat ccagatgggc ctggccactt
     2461 acacgcagaa gccgctgacc cgcacgatcc acgaagcccg ccaactcatg cttgccgccc
     2521 gcaaggcgaa ggtcgtgacc cagatgggca accaggggca cagcggctac ggctaccggg
     2581 ccctcgtcgc gctggtgcag gggggcgcga tcggcagaat caaggaggcc cacgcctggt
     2641 cgaaccgccc gatctggccg caggggatcg accgcccgcc gggcagctcg ccggtgcccc
     2701 agacgctgaa ctgggatctc tggctcggcg tcgcgccgga gcggccgtat gtcgataagg
     2761 cgtatgctcc cttcaattgg cgcggctggt acgacttcgg cgcgggcgcc ctgggcgaca
     2821 tg
//
LOCUS       DASRJB010000246        14518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_70425, whole genome shotgun sequence.
ACCESSION   DASRJB010000246 DASRJB010000000
VERSION     DASRJB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70425"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..1889)
                     /locus_tag="VFB80_06700"
     CDS             complement(480..1889)
                     /locus_tag="VFB80_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN33490.1"
                     /translation="MFLLRTFQLGLKSLLLHPMRSLLTVLGIFIGVASVIWLLAIGEG
                     ISQEAQRQIEGLGADNIIIRSIKPPNEILSSSDSAIPYGLRREEFDMLVQTIPTIQSA
                     LPIREIRRTFTFDGRNEDGRLVGCTPEYFEVTRLELDRGRLLTEADIRDAHNYCVLSS
                     GIAERLFPYQDPLGRRVYLSENRDYYEVVGVLLYRTPTAAIGGSLEAQDFNTDIYIPI
                     TTMRQRVGDYVYTRRGSSETSEIVELNQITLRVKSVKEVRATADLVRKTLHVPEQSRS
                     KDPKKLLAERTQAAVTDGALIESPPRVDVAVIVPEELLEQARVTRMMFMVLMGMIAAI
                     SLVVGGIGIMNIMLATVTERTREIGIRRALGATRSHIILQFLVETISLSVVGGLTGIL
                     AGLLCPAVIVGARALANRYAERVMEDLPEVVQRVEPQIVLASLPLAFGISVVVGVVFG
                     LYPAYRAAQMDPIEALRHE"
     gene            complement(1977..2735)
                     /locus_tag="VFB80_06705"
     CDS             complement(1977..2735)
                     /locus_tag="VFB80_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN33491.1"
                     /translation="MTAAARLVNITKNYVLKSETVRALRGVTFEVPAGDYVAIMGPSG
                     SGKSTLLNLLGCLDKPTSGEFFLGNDDVARMSDDQLAYIRASRIGFVFQSYNLIQQYT
                     VLENIEVPLLYQGRLTSKGRRRCRELADMVGLGDRLGHRPSQLSGGQQQRVAIARSLV
                     NDPYFILADEATGNLDSVTSEEILAMFERLNDEGRTIIMVTHEDDVAAHAKRIIRLFD
                     GLLQTDRHNERRVRGRITHRPVESAEPPVAAEVA"
     gene            complement(2732..4600)
                     /locus_tag="VFB80_06710"
     CDS             complement(2732..4600)
                     /locus_tag="VFB80_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZN33492.1"
                     /translation="MPTSSGQLRRRPLRLAPRRGNTFTFSLVALVVVSGLAFTGWWFL
                     GRGTGDTTPVFLTNTVVEGAYDYVVIEQGAVESATNTELRCEVRSRSGGGGGGGGGPG
                     GGGGGTGGSSTTIIDVVPEGTMVKEGDLVVELDDSALKLEQNGQEIQVSNRRLLLAQA
                     KNKLNAAMIVKKEYLQGVFVDQEKLIKSEVFKAEQTLRTAEQGLKSAKELAAKNIITG
                     LQLEAAQFVVDDAKNLKDAAENRLKTLRELTKERTVSDHDANIKSAEADVEAQELSLK
                     LELEKLQDIQDQIKKCKIYAAKPGQVVYANEYDMWRSSSSSQFVVTPGAMVRERQVII
                     RLPNADDMQIKATVNEARVTLIRPGLPVTIRVDALKDTVIEGVVTKVNQYAEPSGRST
                     ATIKKYATLIKILNPVPELRVGMNAEVRIHVERKPSALQVPVQALAEVKGHYFTLVWN
                     GDDYETREVKIGSSNDKVATIESGLSKNEVVVMNPRSAGSILKLPAIADPAPIVDGEI
                     RRNAPGEVRLAGGPGEGKGGKGGGKGKGGGFNAATMVDRALESDEDKDGKLSVAEIGK
                     MDERGKRWLEGADSNSDGLLERREMLTAAAAFAQRMREAGGKRGGGGPPEPAGGGE"
     gene            5153..5293
                     /locus_tag="VFB80_06715"
                     /pseudo
     CDS             5153..5293
                     /locus_tag="VFB80_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
     gene            complement(5527..7443)
                     /locus_tag="VFB80_06720"
     CDS             complement(5527..7443)
                     /locus_tag="VFB80_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN33493.1"
                     /translation="MIASARYRWPKASSQAVLLAAALAAGCAQQRYVTVHDQPRNVLA
                     SPLKLLARSGPKPTERTQQLLRRYDLLALQEKKPEVALARLEQQIKDDPDPDKICSYA
                     ELSYLQANRLEERGKPEEALDFYGAAVAHAYWFLLDPNLDRFRNPYDPQFRRACDLYN
                     ESLASAMRLVIATKGLHPGRTQTIKTGKQQFEVQVALRGPWRPEDIEKLEFVSRVEIE
                     GGLSNHYHTYGLGVPLIAVRGRREAQSPVERYYPPGLCFPVTAFLRVENQTPQQAAGD
                     VHRCTLELYDPLFSSDIAVCNRLVPLETDLTTPLAYFLDQPSFKESDIANLGLFKPNE
                     AQGIKGLYMVEPFDPNRIPVVFVHGLWSSPTTWMEMFNDLRAFPEIRGRYQFWFFLYP
                     SGQPFWLSAARMRETLAEVRLELDPQGLNPNLDQLVLVGHSMGGLVSKLQTLESGDDF
                     WRILSDRPIEELKASPEDRARLAKSFYFHPNPSVKRVVTIGTPHRGSEFANSFSREVG
                     RRLIALPEMMLELGNKLTLSNPGYFRNKELLTMTTSIDSLAPDCPIFPVILSAPRANW
                     TTYHNIVGVVPKETFVGRVSEEGDGVVGLASAHMEYVASELIVEADHLTVHRHPLSIL
                     EVRRILLEHLNVAR"
     gene            7690..8544
                     /locus_tag="VFB80_06725"
     CDS             7690..8544
                     /locus_tag="VFB80_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HZN33494.1"
                     /translation="MGDLVLALGVLTLAALGLFLLTVRWTRCWSPRACDLAGLAVVVL
                     LLVYIRTLWYDLRLAHWLPLASLVVLGNWLPLFAAVLAGLVWNRTSDRPLRRRIAVLE
                     LTAAGILAAIYPLLGASPACGERWDRLNTCLQTTPYTCSPAAAATLLRRHGIAASEQE
                     MARLCLTRKGTSWQGLYRGLKLKTAGTPWDVEVCRLPAAELLASRSGPMILSVGLGRS
                     AAADGAYTREFGWVPGVSHSVVLESVSETGCAVIADPSCEMCREYWDRRMLAALWRGY
                     GLRLVPRR"
     gene            8620..9387
                     /locus_tag="VFB80_06730"
     CDS             8620..9387
                     /locus_tag="VFB80_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33495.1"
                     /translation="MRKYARLAVVVLLALAGAGAISLFSLYRASRHVPVFYERALAVQ
                     PEAQKAAGQKFEQEALSLHNQVRQPGRWEARFTQDEINGWLAADLPVKFPQALPKGAS
                     DPRMALSAGQVQLAVRYERGATKTVVSLSGQVQLTQEPNELAVRIDGVRAGALPLPLA
                     EFRDEIAERAAKAGVPIRWTEAEGVPVALVRLPLDPREFGGRRLVVEELRLTEGELFV
                     AGRTELPPDAPQPAPGEPVDSGEPIRAAAPEPETRQR"
     gene            9391..10083
                     /locus_tag="VFB80_06735"
     CDS             9391..10083
                     /locus_tag="VFB80_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZN33496.1"
                     /translation="MRIAEIFSSRQGEGVLTGTPSVFVRASGCNLRCWFCDTPYTSWQ
                     PEGDNWSVEQIVAEVARLRADVRHAVITGGEPMLYAELVPLCEHLKTADLHITIETAG
                     TVDQPVACDLMSISPKLASSTPPAAIAGEWTGRHERDRHQPDVIRRLIADYEYQLKFV
                     IDTPADCEAVQQWLAEFPQADRSRVLLMPQGIDLTTLGQMASWLEPYCEQHGFVFCPR
                     KHIEWYGATRGT"
     gene            complement(10162..11706)
                     /locus_tag="VFB80_06740"
     CDS             complement(10162..11706)
                     /locus_tag="VFB80_06740"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZN33497.1"
                     /translation="MTTTRCCAAISLLLVALSTPRWAAAQNDAAAKAAAAVENAVSAG
                     SELFHKQFAAGEPIGQSGDGLGPVFNQVSCIACHRQAGVGGAGSVDLNASMLSIEPIK
                     FETLPDRDNFAKVLRRIHPGFVSDDGQVTNNVILHRFGAGDKYLAMREKLVGSSIPFQ
                     PTDDQRREIQQTLARAPVQPASGARPLRLVVTQRNTTALFGAGLIDTIPDAVLVGLAA
                     IQSTHPEVSGRVAPIKLTRAGRFGWRGQTERLHDFVLGACANELGLEVPGHPQPLDPL
                     RPKYRSPGLDLSPQQCLSLTAFVASLPQPKFALPDDGEKRQVVERGYKAFHGVGCGVC
                     HVENIGPVQGVYSDLLLHDMGLGLADPVLAQATMQQVEQVPISADARYKQPGRIIGDE
                     TIVKPNPVPYYSSRPPTIDRGTTYATEGGGLATVVDSPRDKRICILTRYEPVATNLAQ
                     EWRTPPLWGLADSAPYLHDGRAESVLEAIAQHGGEAEPCLARFLALPTGERLAVLEFL
                     GCLRAP"
     gene            complement(11693..12958)
                     /locus_tag="VFB80_06745"
     CDS             complement(11693..12958)
                     /locus_tag="VFB80_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33498.1"
                     /translation="MALVRHLRSASASAVGLCFLLTSATVAQETVTWLADRGEAIAQA
                     RKQGKLLLVVQLSGDFARNAADSSEAKTYRGTALADPGVARVLHERYVVTYQQVGEAE
                     SLRKLHPVKTQPVRTEFAATYVCLPDLRVLHFIPGFVTSAELLEELKWAGMSYRAIVE
                     LPAMDEGPAARRAHLAAAAKGDVALFERAFKTRWTGEALAAGPSTVDLPAALSAARGT
                     LELSLSQRLGKSWQRTPAQDAIAALTAHGGLGREMAHLTLGEFPLVPLGDLERPAFEA
                     CTGDRFWQASRRRQSLANWWTAGASSGKPLMLVVADDAFAPGAGESSALAWPPAKADA
                     LPLHAQFVVSTLSLDEFAALATDAKWEPQTYRSAAPPRFMVFAAGGKPSALLGKGTSL
                     NRLAQAMTAASGAGARTALAAGESIDDDH"
     gene            complement(13084..13428)
                     /locus_tag="VFB80_06750"
     CDS             complement(13084..13428)
                     /locus_tag="VFB80_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN33499.1"
                     /translation="MTPTQLMQAIDIQLAHVWMVRAFLKHSEEAAEDDELADVHRELY
                     DFMLALGGSLKDGNADEYLRLAGKKFGKLKRATETFARIQPEVSQHTNFAMAAASLTA
                     AVAEIGRLLGQP"
     gene            13464..13628
                     /locus_tag="VFB80_06755"
     CDS             13464..13628
                     /locus_tag="VFB80_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33500.1"
                     /translation="MSNEFVRAVVFVASAGSTITCGKKRLPAIMRIMRAKALPVVGDY
                     RVAVSMRAYV"
     gene            13936..>14518
                     /locus_tag="VFB80_06760"
     CDS             13936..>14518
                     /locus_tag="VFB80_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33501.1"
                     /translation="MSIRPLALACLLVVGSAVTALACLWDYDTLQMERLRFPGALEII
                     TGKFVRHSKAYYEWRITDRKKKLESQPDDPALLDDLAVAYAKLGRPEEGIALLEPTLA
                     SQPDRYETLSNLGTLLFFAGRLEESKTHIRRALEINPDAHFGRERYQLLLTEYVQQSS
                     LKDAGVMSTEERHPLLDRTVGFARHVLVAKFGQT"
BASE COUNT         2452 a         4803 c         4694 g         2569 t
ORIGIN      
        1 acccgcgtgt taggaaacac tcgtgattga actggacact taggtaaaga cgccgacggc
       61 ggacagtccg ccggtcggac ggagacgaga ggcaggcggc ggggcggagg gccgcgccaa
      121 cgaggcttgg aaccggcgca gccgcgggtc gtccgcgtgc ggcgccgtaa gcgtcactgc
      181 cgagcgatag tcaccagcgc tgacggctcc ctgcactggt gcgtggtccg cccgacgcga
      241 tgggtgcgtg gagcggagcc ggttgcgggc ccccgttggt ttccaccatc cggtgcatcc
      301 gctcggacac gagcggaagg caggcccgca gcgacgaatc aggaatcccg tgcgacgtcg
      361 caatccgccg cagcgatgtg aatcgccgcg gcgttagggc aatctaggcc gcgcctgaca
      421 acggttcaac aagctagctg gcagatttcc ggaaatgatg cagtgctgcg actgcaatgt
      481 cactcgtgcc tgagcgcttc gatgggatcc atttgcgccg cccgataggc ggggtagagc
      541 ccgaacacga cgccgacgac gacagagatg ccaaaggcca gcggcagcga ggccaggacg
      601 atctgcggct cgacgcgctg cacgacttcg ggcaggtctt ccatcacccg ctccgcatac
      661 cggttggcca gcgcgcgtgc gcccacgatc accgccgggc acaagaggcc cgccaggatg
      721 cccgtcagcc cgcccacgac ggacagcgag atcgtctcga ccaggaactg gaggatgata
      781 tgcgaccgcg tcgcgcccag ggcccgacga atgccgatct cgcgggtccg ctcggtcacc
      841 gtggcgagca taatgttcat gatcccgatg ccgccgacga ccagcgaaat ggcggcgatc
      901 atgcccatca gcaccatgaa catcatccgc gtcacgcggg cctgctcgag caattcctca
      961 ggcacgatca ccgcgacatc gacccgcggc ggcgattcga tgagtgcccc gtccgtcacg
     1021 gcagcctgcg tcctctcggc cagaagcttc ttggggtcct tcgagcgcga ctgctcggga
     1081 acatggagcg tcttgcggac caggtcggcg gtggcccgca cctccttcac cgacttgacg
     1141 cggagcgtga tctggttgag ctcgacgatc tcggaagtct cagagctgcc gcggcgggta
     1201 tagacgtagt cgccgacgcg ctgccgcatc gtggtgatcg ggatgtagat gtcggtgttg
     1261 aagtcctggg cctccagcga gccgccgatg gcggccgtgg gcgtgcggta gagcagcacg
     1321 ccaacgacct cgtaatagtc gcggttctcg gacaggtaaa cccggcgtcc gagcgggtcc
     1381 tgatagggaa acagccgctc ggcgatgccg ctggagagca cgcaatagtt atgggcgtcc
     1441 ctgatgtcgg cctcggtgag caggcggccg cggtcgagct ccagccgcgt gacctcgaag
     1501 tattcgggcg tgcagccgac gaggcggccg tcctcgttgc ggccgtcgaa ggtgaaggtg
     1561 cggcggattt cgcggatcgg cagagccgac tggatcgtcg ggatcgtctg cacgagcatg
     1621 tcgaactctt cccgccgcag cccataagga atcgcggagt cggagctgct caggatttcg
     1681 ttcggcggct tgatcgagcg gatgatgatg ttgtcggcgc ccaggccttc gatctgccgc
     1741 tgggcctcct ggctgatccc ctcgccgatc gctagcagcc aaatcacgct cgccacgccg
     1801 atgaagatgc ccagcacggt caagagcgac cgcatcgggt gcagcaggag gcttttcagg
     1861 ccgagctgga atgtgcgcag cagaaacatg ggaccggtgg tcggttattc agtcggtcgg
     1921 ttggtcagtg agtccgtgac cgatcgaccg actgaccggc cgaccgaccc accgacctat
     1981 gctacctccg cggcgacggg cggctcggcg gattcaaccg gccggtgcgt gatccggccg
     2041 cgcacccggc gctcgttgtg ccggtccgtc tgcagcaggc cgtcgaacag gcggatgatc
     2101 cgcttggcgt gggccgccac atcgtcttcg tgcgtgacca taatgatcgt ccggccttcg
     2161 tcgttcagcc gctcgaacat cgccaggatt tcctcgctgg tcaccgagtc caggttaccc
     2221 gtggcctcat cggcgaggat gaagtagggg tcgttcacca ggctgcgagc gatcgccacc
     2281 cgctgctgct ggccgccgga gagctggctc ggccggtgcc ccagccggtc ccccaggcca
     2341 accatgtcgg ccagttcgcg gcagcggcgg cggcccttgc tggtcagacg gccctgatag
     2401 agcagcggaa cctcgatgtt ctccagaacc gtgtattgct ggatcaggtt gtacgactga
     2461 aagacgaaac cgatccgcga ggcgcggata taggccagct ggtcgtcgct catgcgggcc
     2521 acgtcgtcgt tgcccaggaa gaactcgccg gaggtgggct tgtcgaggca gcccagcaaa
     2581 ttgagcagcg tgcttttgcc ggagccggac gggcccatga tcgcgacata gtcgccggcg
     2641 ggaacttcaa acgtcacgcc gcgcagggcg cggaccgtct cgctcttgag gacgtaattc
     2701 ttggtgatgt tcaccaatcg ggcagccgcc gtcattcgcc ccctcccgcc ggttcgggcg
     2761 ggccgccgcc accacgcttg ccgcccgcct cgcgcatccg ctgggcgaac gcggcggcgg
     2821 cagtcagcat ttcgcggcgt tcgagcaggc cgtcggagtt actgtcggcg ccttcgagcc
     2881 agcgcttgcc gcgttcatcc atcttgccga tttcggcgac ggacagcttg ccgtccttgt
     2941 cttcgtcgct ctccagggcc cggtcaacca tggtcgcggc gttgaagccg ccgcccttgc
     3001 ccttgccacc tcctttgccg ccctttccct caccaggacc accggccagg cggacttcac
     3061 ccggagcgtt ccgccgaatc tcgccgtcca cgatcggggc cggatcggcg atcgccggca
     3121 gcttgaggat gctcccggcc gagcggggat tcatcacgac cacttcgttc ttcgagaggc
     3181 cgctctcgat cgtggcgacc ttgtcgttcg acgagccgat cttgacctcg cgggtttcgt
     3241 agtcgtcgcc gttccagacg agcgtgaagt agtgcccctt gacctcggcc agcgcctgca
     3301 cgggcacctg tagcgccgag ggctttcgct cgacgtgaat ccgcacttcg gcgttcatgc
     3361 ctacccgcag ctcgggcacc ggattcagga ttttgatcag cgtggcgtac ttcttgatcg
     3421 tcgcggtcga gcggccgctg ggctcggcat attgattgac cttcgtgacc actccctcga
     3481 tgaccgtgtc cttgagggca tcgacgcgaa tggtcaccgg caagcccggc cggatcagcg
     3541 tcacgcgggc ctcgttcacc gtggccttga tctgcatgtc gtcggcgttc ggcaggcgaa
     3601 tgatcacctg ccgctcgcga accatcgccc ccggcgtgac gacgaactgc gacgagctgc
     3661 tggagcgcca catgtcgtat tcattggcat agacgacctg gccgggcttc gccgcataga
     3721 tcttgcactt cttgatctga tcctgaatgt cctgcagctt ctccagttcg agcttgaggc
     3781 tcaattcctg ggcctccaca tcggcctcgg ccgacttaat attcgcgtcg tggtcgctga
     3841 ccgtccgctc tttggtcaat tcgcggagcg tcttgagccg gttctcggcc gcgtccttga
     3901 gattcttggc gtcatcgacg acaaactggg ccgcctcgag ctgcaggccc gtgatgatgt
     3961 tcttcgccgc cagctccttg gccgacttca gcccctgctc ggcggtccgc aatgtctgct
     4021 cggccttgaa cacctccgac ttgatcagct tttcctgatc gacgaacacg ccctgcaggt
     4081 actccttctt cacgatcatc gcggcgttca atttgttctt ggcctgcgcg agcagcagcc
     4141 ggcggttgct gacctggatc tcctggccgt tctgctcgag cttcagggcg ctgtcgtcca
     4201 actcgacgac caggtcgcct tctttcacca tggtcccttc gggtacgacg tcgataatcg
     4261 tcgtgctgct gccgccggtg ccgccgccac cgccgcccgg tccgccgcca ccgccaccgc
     4321 cgccgccgct acgcgagcgg acctcgcagc gcaactcggt attcgtggcg ctttccaccg
     4381 cgccttgttc aatgacgacg tagtcgtacg ccccctcgac cactgtattg gtcaggaaaa
     4441 ccggcgtggt atcgccagtg ccgcgaccca agaaccacca gcccgtgaac gccagcccgg
     4501 aaacgacgac cagcgccaca agcgaaaacg tgaacgtgtt gccgcggcgg ggggctagcc
     4561 tgaggggacg gcggcgaagc tgcccggaag aggttggcat gtcactactc ccacgatgac
     4621 gctagctcga acaggcagga atcccggtcg gacaatggcg ggcggctgtc ggcggcaacc
     4681 gtcgggcggg acaaatcacc aatttccgat gtattgtaac cgcgccaaaa cgggctgtca
     4741 cgctgtttat cgccagggct tttcggggga attgctaccc ggaaaaccca acccaagccc
     4801 taccgccatc aatccccaag cattcctggt cctaggaatc gaaccccgca ccaggctgag
     4861 agtttcggcg acggaaaacc atcgtttaac cgcagccgca aggcgcaggc tctttccgag
     4921 tagccgccgt cgcctcctgc tccggattaa acggatttgg aaagcttttc acgcgatttg
     4981 taagtgcgaa accaaatgtc caccagccgc ccacggttgc ggacacttct aacttgctcg
     5041 gattgctgga cttgcgccgg ccaccggctt ccggcgagcg gtttggcacg ccctctgcgt
     5101 tagggtgggt cgaacgacgc tcgtcttcgt ctcatcgcaa aggagtgtgg ccatgctcgt
     5161 cctgaccagg aagctgcagc agcaaatcaa gattggggag caaatcacgg tgaccattct
     5221 gcgggtcaaa ggaaacacgg tccgcgtggg catcactgcc ccgcgcgacg tgcgcgtcat
     5281 tcgcggcgaa ctgccgaaga cggccgcacc cggcagcgaa cagcaactcg cggaagcagt
     5341 gtccgcgacg agagacgaga ccgtggaggc ggcggacgat tccgccggcc ccgtagccgc
     5401 cagcctgaca acgcccgctg ccctgccgcc ggccgcgcac ctgccgctgc gccggatcgc
     5461 caaccgccac ggcgctgctc ctctcaagcg gatcgtcgcg gcctgctctt cgctggcgaa
     5521 gtagcgctac cgtgcgacgt ttaaatgctc cagcagaatc cggcgaactt ccaggatcga
     5581 gagcgggtgg cgatgcacgg tgagatggtc cgcctcgacg attagctccg acgccacgta
     5641 ttccatgtgg gcgctggcca ggccgacgac gccgtcccct tcttccgaca cgcggcccac
     5701 gaacgtctcc ttgggcacca cgccgacgat attgtggtag gtcgtccaat ttgcccgcgg
     5761 cgcgctgagg atcacgggaa agatcgggca gtccggcgca agggagtcga tgctcgtggt
     5821 catcgtcagc agctctttgt tgcgaaaata gcccgggttg ctgagcgtta gcttgttgcc
     5881 cagttccagc atcatctcgg gcagcgcgat tagccggcgg ccaacctcgc gcgaaaagct
     5941 gttggcaaac tcgctgccgc ggtgcggcgt gccgatcgtc acgacccgct tcactgacgg
     6001 gttggggtgg aagtagaagc tcttggccag gcgagcccgg tcctcgggcg aggccttcag
     6061 ctcttcgatg ggccggtccg agagaatccg ccagaaatcg tcgccgcttt ccagcgtttg
     6121 cagcttgctc accagcccgc ccatgctgtg ccccaccagc acgagctgat ccaggttcgg
     6181 attcagcccc tgtgggtcca attccagccg tacctcggcc agcgtttcgc gcatccgcgc
     6241 ggcgctgagc cagaacggct gccccgacgg atagagaaag aaccagaact ggtagcgacc
     6301 gcgaatttcc ggaaatgcac gcaggtcgtt gaacatctcc atccaggtcg tcgggctcga
     6361 ccacagcccg tgcacgaaca ccaccggaat gcgattgggg tcgaacggct ccaccatgta
     6421 gagccccttg atcccctgcg cctcgttcgg cttgaatagc cccagattcg cgatgtcgct
     6481 ctctttgaac gacggctgat cgaggaaata ggccagcggc gtggtgaggt cggtctccag
     6541 cggcaccagg cgattgcaga cggcgatgtc gctgctgaac agcgggtcgt acaactccag
     6601 cgtgcagcgg tgcacgtcgc cggcggcttg ctgcggcgtc tgattctcga cgcggaggaa
     6661 cgccgtgacc gggaagcaga ggcccggcgg atagtagcgc tcgaccggcg actgcgcctc
     6721 gcgccggccg cgaacggcga tcagcggcac gcccaggccg taggtgtggt agtggttcga
     6781 aagcccgcct tcgatctcca cgcggctcac gaactccagc ttctcgatgt cctccggccg
     6841 ccacggtccg cggagggcga cttgcacctc gaattgctgc ttgcccgttt tgatcgtctg
     6901 cgtcctgccg gggtgcaggc ccttggtggc gatcaccagc cgcatcgccg aggccagcga
     6961 ttcgttgtaa aggtcgcagg cccggcgaaa ctgcgggtcg tacgggttgc ggaagcggtc
     7021 gaggttcggg tcgagcaaga accaataggc gtgggccacc gcagcgccgt agaagtcgag
     7081 cgcctcctcc ggcttgccgc gctcctccag tcgattggct tgcagatagg agagctcggc
     7141 gtacgagcag atcttgtccg ggtcggggtc gtccttgatc tgctgctcga gccgcgcgag
     7201 tgcgacttcg ggcttcttct cctggagcgc caagaggtcg taccgccgca agagctgctg
     7261 cgtccgctcc gtcggcttcg gaccgctgcg ggccagcagt ttcagcgggc tggccagcac
     7321 gtttcgcggc tgatcgtgga cggtgacgta ccgctgctgc gcgcaacccg cagctagcgc
     7381 agccgccagc aggaccgcct gcgaggaagc cttcggccag cggtaacgag cagatgcaat
     7441 catggcgcag tgcgagacca cgcgcccaat ggcgcaaaaa cggcccgcga gaattaccgg
     7501 cgtcggcaga ctgcgacaag gtcatttcga tcaacagcta gcggttgacg atctcgtcat
     7561 tggagtgctg tggacccatc cgccgatgat gagtaggatt ctcacaattg cctcaagatt
     7621 cacgatgagt tcattgaaac ttaacaatcg gtcgtcttga gggtttggcc gcggccggag
     7681 gcccgcccga tgggtgacct cgtgcttgct ctcggcgtgc ttacgctggc ggcgctcggg
     7741 ctgtttctgc tgacggtgcg ttggactcgc tgctggtcgc cgcgggcctg cgacctggcc
     7801 ggtctggcgg tagttgtgct gctgctcgtc tatatccgca ctctgtggta cgaccttcgg
     7861 ctggcgcatt ggctgccgct ggcgagcctg gtagtgcttg gcaattggct gccgctgttc
     7921 gccgcggtgc tggcgggact cgtctggaat cggacatccg accggccgct gcggcggcgg
     7981 attgcagtgc tggagctgac cgcggcggga atcctggccg cgatttatcc gctcctcggc
     8041 gcaagccccg cgtgcggcga gaggtgggac cggctgaata cgtgcttgca gaccacaccc
     8101 tacacctgct cgccggcagc cgcggccacg ctgctccgcc ggcacggcat cgccgccagc
     8161 gagcaggaaa tggcccggct ctgcctgacc cgcaagggca cgagctggca aggcctctat
     8221 cgcggcctca agctgaaaac ggcaggcacg ccgtgggacg tcgaggtctg ccgcctgccg
     8281 gccgccgagc tgctggccag ccgcagcggg ccgatgattc tcagcgtcgg cttgggccgc
     8341 tcggccgcgg cggacggcgc ctacacgcga gagttcggct gggtcccggg cgtgagccat
     8401 tccgtggtgc tcgaaagcgt cagcgagacc ggctgcgcgg tgatcgccga tccctcgtgc
     8461 gagatgtgcc gggaatactg ggatcgccgg atgctggctg cactgtggcg cggctatgga
     8521 ctgcgcctcg taccgcggcg ctagctgcga cgcgcaattt ggcggccgcg aaccgtgcaa
     8581 aacgtataat ggtgacggtg ctcttgccat aggaccagca tgcggaagta cgcgcgtttg
     8641 gcggtggtcg ttctcctggc gttggcagga gctggcgcga tttcgctttt cagcctgtac
     8701 cgggcgtcgc ggcacgtgcc cgtgttctac gagcgggctt tggccgtgca gcccgaggcc
     8761 cagaaggccg ccgggcaaaa gttcgagcag gaggccctct cgctgcacaa ccaggtccgc
     8821 cagcctggcc gctgggaagc ccgcttcact caggacgaaa tcaacggctg gctcgcggcc
     8881 gacttgcccg tcaaattccc tcaggcattg cccaagggcg cgtccgatcc gcggatggcc
     8941 ctgagcgctg gccaggtcca gctcgccgtt cgctacgagc gcggggcgac gaaaacggtc
     9001 gtctcgctca gcggccaggt gcagctcacc caggagccca acgagctggc cgtgcggatc
     9061 gacggcgtgc gtgcgggcgc attgcccctg ccgctggcgg aattccgcga cgagatcgcc
     9121 gagcgggccg cgaaagccgg cgtgcccatt cgctggaccg aggccgaggg cgtgcccgtt
     9181 gcgctcgtgc gactgccgct ggatccgcgc gagttcggcg gccggcggct ggtggtcgaa
     9241 gaactgaggc tgactgaggg cgagctgttc gtcgccggcc ggaccgaact cccgcccgat
     9301 gcgccgcagc ccgcgccagg cgagcccgtg gacagcggcg agccgatccg ggccgccgcg
     9361 ccggaaccag aaacgcgcca gcgatagccc gtgcgaatcg ccgaaatctt cagttcgcga
     9421 cagggcgaag gcgtgctcac gggcacgccc agcgtcttcg tccgcgccag cggctgcaac
     9481 ctgcgctgct ggttctgcga cacgccctac acctcgtggc agccggaggg cgacaactgg
     9541 tcggtcgagc aaatcgtcgc cgaagtcgcg cggctccgcg cggacgttcg ccacgccgtt
     9601 atcaccggtg gcgagccgat gctctatgcc gagttggttc cgctgtgtga gcatctgaaa
     9661 accgcagacc tgcacattac gatcgaaacc gcaggcacgg tcgatcagcc cgtggcctgc
     9721 gacctgatgt cgatcagccc gaagctcgcc agctcgacgc cgcccgcggc gattgccggc
     9781 gagtggaccg gcaggcacga gcgcgaccgg caccagcccg acgtcatccg ccggctgatc
     9841 gccgactacg aataccagct caaattcgtc atcgacacgc ccgccgattg cgaagcggtc
     9901 cagcaatggc tcgcggagtt tccgcaagcc gatcgcagcc gcgtgctgct catgccgcaa
     9961 ggcatcgacc tgaccacgct aggccaaatg gcgagttggc tcgagcccta ctgcgaacaa
    10021 cacggcttcg tcttctgccc gcgcaagcac atcgagtggt acggcgcgac ccggggaaca
    10081 tgacggagcg atggagagaa agagagacgg aatcgccatc cgtccctctt cgctccatcc
    10141 ttccctcgct ctgctgcgtg tctacggtgc ccgtaagcag ccgagaaatt ccaacaccgc
    10201 cagccgctcg ccggtaggca aggccaggaa tcgcgcgagg cagggctcgg cctcgccgcc
    10261 gtgctgggcg atcgcctcca gcacgctctc ggcccgcccg tcgtgcaggt agggggccga
    10321 atcggccagg ccccacaagg gcggcgtccg ccattcctgc gcgaggttcg tggccaccgg
    10381 ctcgtaacgc gtgagaatgc aaatccgctt gtcacgcggc gagtccacca ccgtcgccag
    10441 tccgccgccc tcggtggcat aagtcgtgcc ccggtcgatc gttggcggtc ggctgctgta
    10501 gtagggcacc ggattgggtt tgacaatcgt ttcgtcgccg atgatccgcc ccggctgctt
    10561 gtaacgggcg tcggcggaaa tcggcacctg ctcgacttgc tgcatcgtgg cctgggccaa
    10621 aaccggatcg gccaggccca ggcccatgtc gtgcaacagc aggtcgctgt aaacgccttg
    10681 caccgggccg atgttctcga cgtggcacac gccgcagccg acgccgtgaa atgccttgta
    10741 gcctcgctcc acgacttgcc gcttctcgcc gtcgtccggc agggcgaact tcggctgcgg
    10801 cagcgacgcg acgaacgccg ttaacgagag gcattgctgt ggcgaaaggt ccagccccgg
    10861 cgagcggtat ttcggccgca gcgggtccag cggctgcgga tgccccggca cctccaggcc
    10921 cagctcgttg gcacacgcgc cgagcacaaa gtcgtgcagc cgctcggtct gcccgcgcca
    10981 gccgaatcgc ccggcgcggg tgagcttgat gggggcgacg cggccggaga cttccgggtg
    11041 cgtgctttga atggcggcca gtcccaccag gaccgcgtcg ggaatcgtgt cgatcagccc
    11101 cgcgccgaac agggccgtcg tgtttcgctg cgtgacgacg agccgcagcg gccgcgcgcc
    11161 gctcgccggc tgcaccggcg ctcgggcgag cgtttgttga atctcgcggc gctggtcgtc
    11221 ggtcggctgg aacggaatcg acgagccgac tagcttctcg cgcatcgcga ggtatttgtc
    11281 gcccgcgccg aagcggtgca ggatcacgtt attggtgact tgcccgtcgt cggagacgaa
    11341 tcccgggtga atccgtcgca ggacttttgc gaaattgtct ctgtcgggca gcgtctcgaa
    11401 cttgatcggc tcgatcgaga gcatcgaggc gttgagatcg accgaccccg cgccgccgac
    11461 gcccgcctgc cgatggcagg cgatgcagga gacctggttg aagaccggac ccaggccgtc
    11521 gccgctctgg ccgatcggct cgccggcggc gaattgcttg tgaaagagtt cgctgccggc
    11581 gctcaccgcg ttctcgaccg cggcggcggc cttggccgcg gcgtcgtttt gtgcggctgc
    11641 ccagcgcggg gtacttagcg ctacgagcaa caaggaaatc gcggcgcagc atctagtggt
    11701 cgtcatcgat cgattctcct gcggccaagg ccgtgcgggc ccctgcaccc gacgcggcag
    11761 tcatcgcctg ggcgagccgg ttcaggctcg tcccttttcc aagcagtgcg gagggcttgc
    11821 cgcccgccgc aaacaccatg aatcgcggcg gcgcagccga gcggtaggtc tgcggttccc
    11881 atttggcatc ggtcgccagc gcggcaaatt cgtcgagtga gagcgtgctg acgacgaact
    11941 gcgcgtgcag cggcagcgcg tcggccttgg ctggcggcca ggccagcgcg ctggattcgc
    12001 cggcgcccgg agcgaaggcg tcgtcggcga cgaccagcat gagcggcttg ccgctggaag
    12061 cgccagccgt ccaccaattc gccagcgact gacgccgtcg cgaggcctgc cagaagcggt
    12121 ctcccgtgca ggcttcgaac gccggccgct cgaggtcgcc cagcgggacg agcggaaact
    12181 cgcccagcgt taaatgtgcc atttcgcggc ccaatccgcc gtgagcggtc agcgcggcga
    12241 tcgcatcctg cgccggagtt cgctgccagc tttttcccag ccgctgggag agcgacagct
    12301 ccaaagtccc gcgggccgcg gaaagcgccg ccggcagatc gaccgtcgaa ggtccggctg
    12361 ccagcgcttc gccggtccaa cgcgtcttga acgcccgctc gaacagcgcc acatcgccct
    12421 tcgcagccgc ggccaggtgc gcgcgccgcg ccgctggccc ttcgtccatc gcgggcagct
    12481 cgacgatggc ccggtaactc attccggccc acttcaactc ctccagcagc tccgccgagg
    12541 tgacgaaccc cggaatgaaa tgcagcaccc gcaggtccgg caggcagacg taagtcgcgg
    12601 caaactcggt ccgcaccggc tgcgtcttca ccggatgcag tttgcggagc gattccgcct
    12661 cgccgacctg ctgatacgtc accacgtatc gctcgtggag cacccgcgcc actcccgggt
    12721 cggcgagtgc ggtaccacga taggtcttgg cctcgctgga gtcggcggcg ttccgcgcaa
    12781 aatcgcccga gagctgcacc accaaaagca gcttgccctg cttgcgggcc tgggcaatcg
    12841 cctcgccgcg atcggccagc caggtcaccg tttcctgggc gacagtggcc gaagtcagca
    12901 ggaagcagag gccaactgca ctggcgctcg ccgagcggag atggcgaaca agagccaccg
    12961 ctgcctctta tcgggttgtg cccctttgcc ggcggcgtgt agttggaaaa ggaaccgctg
    13021 gcgacttgca acctaaccca tgccacctcg ccgagcaagc gctgcacagc gaggacgcaa
    13081 atactacggc tgaccaagca gtcgcccaat ctccgccacc gccgccgtca gcgacgccgc
    13141 ggccatggca aaattcgtat gctggctgac ctccggctga atccgggcga acgtttcggt
    13201 cgcccgcttg agcttcccga actttttgcc ggccagccgc aagtactcgt cggcattgcc
    13261 atctttcagc gacccgccca gggccagcat gaagtcgtac agctcacgat gcacgtcggc
    13321 caactcgtca tcctccgccg cttcctcgct gtgcttcaga aacgcccgca ccatccagac
    13381 gtgggccaac tggatatcga tcgcctgcat gagttgggtc ggagtcatgg tgtcgctggc
    13441 caacggcccg cgcctatgcg cgcatgtcca acgaatttgt tcgggcagtg gtttttgtcg
    13501 caagtgctgg ctccacaata acttgcggca agaaacggct gccagccatt atgcgcatta
    13561 tgcgcgccaa ggcactgccg gtggtcggcg attaccgcgt cgccgtctcc atgcgcgcat
    13621 atgtgtgagt tgttcccgaa ggttgttttt cgccgcaacc tctgacggca tcagcacttg
    13681 cggcgaaaaa actgccttgg caattatgcg cattatgcgc gcaagcgccc tgttcggcct
    13741 cgatcattca tgacttttca aagaacatca cccgccgcag cgaggggcga ggtccagcca
    13801 agagaattgt agcaactgta tagataaggt caagaaagtg gacggcgatc cgcgagcgaa
    13861 gtttcatcgt gccgggttgt tttcgggccg gtataacggg cttacgactc atccggccgg
    13921 tagtgaggca cctccatgtc gatccgtcct ctcgccttgg cgtgcttgct cgtagtgggc
    13981 tcggctgtaa ccgcgctggc ttgcctctgg gactatgaca cgctccaaat ggagcggctg
    14041 cggtttccgg gggcgctgga gatcatcacg ggcaagttcg tccgccacag caaggcctac
    14101 tacgagtggc gaatcaccga ccggaagaaa aagctggagt cgcagccgga cgatcccgcg
    14161 ctgctcgacg atctggcggt tgcctatgcc aagctcggcc ggcccgaaga gggcatcgcc
    14221 ctcttggagc cgacgcttgc cagccagccg gaccgctatg agacgctctc gaacctgggc
    14281 acgctgctgt tcttcgcggg gcggctcgag gagagcaaga cccacatccg ccgtgccctg
    14341 gagatcaatc ccgacgccca tttcggccgc gagcggtatc aactgctgct gaccgaatac
    14401 gtgcagcaaa gcagcctgaa ggacgcgggc gtgatgtcca ccgaggagcg acatcctctg
    14461 ctggacagga ccgttggctt tgcaaggcac gtgctggtgg cgaagtttgg ccagaccg
//
LOCUS       DASRJB010000247         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3453386, whole genome shotgun sequence.
ACCESSION   DASRJB010000247 DASRJB010000000
VERSION     DASRJB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453386"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFB80_06765"
     CDS             <1..273
                     /locus_tag="VFB80_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33502.1"
                     /translation="PPLRGGPATESRATAGPGEAQFVASLTQQTPLETAGEIDLYLPP
                     GQTAMVVAVSRSSRRIVAWSLAAQRGEGEWSLFHFRPAGPNPERVP"
     gene            270..1031
                     /locus_tag="VFB80_06770"
     CDS             270..1031
                     /locus_tag="VFB80_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33503.1"
                     /translation="MNGSKIALFAAAALGIAGAALNLAYLTGKAQQAEKVSYIGIRKG
                     AIIRRGDRFSEDHLVPIDIPKSHVGNLERVAIRYADRGTVVGMSAVKDYEGDELLLRG
                     DLDTPPATLELKSTERAMWIPVDTRTFVPSLVNPGDLVSFLVGDAPTPAMPSEAPGNA
                     EDGDTTQPPLPAVPLSGRGELIGPFRVLSVGNRLGRTDVLKASGVPQLQENVITISVT
                     VQGDQLEPKAAKLWKMLHLSGSRQAGVILHPSTKN"
     gene            1060..>2612
                     /locus_tag="VFB80_06775"
     CDS             1060..>2612
                     /locus_tag="VFB80_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN33504.1"
                     /translation="MKIWFNKLHDSRRSVVETTAAEITLGRDATCDVVLASPLVSRRH
                     AVVRISGERLELENTGLNSLLVGAAEIFGGQRISFGLGERLRIWPFTITFEKEQAPTL
                     SRTELEAHIRAQLADLELEIHKLLLARLDLVEIESQRQTLRERVLLLENHIEDICREL
                     GVFHEQNEVLLAEIAGVALRDLLIHQLVMEADWQAPPDLAMLTSNEFDVPATLVPERE
                     TELHALVHHLRTKLDLASEKDADRRIERVEKGAAETFPLIRPHLHAELRKYLIQRTLK
                     KDLKDTVFGFGPLQDLLRAPNINEIMVVAHDQIYFERNGVIELSGRRFLSAAVTEAII
                     ERIVAQVGRRIDKSQPLVDARLPDGSRVNAIIPPLAVRGPCLTIRKFGARQFTVEDLI
                     ERDTLTRPAAEFLRACVIDERNILVSGGTGSGKTTLLNVLSSFIPHKQRIVTIEDTTE
                     LKLLQEHVVSLESKLANVEGTGAYTIRDLLKNALRMRPDRIIVGECRGPEALDMLQAM
                     NTGHDGSLTT"
BASE COUNT          479 a          873 c          861 g          399 t
ORIGIN      
        1 ccgccactcc gtggcgggcc cgcgacggag tcgcgggcta ctgccggtcc cggcgaagcg
       61 caattcgtgg ccagcctgac gcagcaaacg ccgctggaga ccgcgggcga gatcgatctg
      121 tacctgccgc cgggacaaac cgcgatggtc gtcgccgtca gccgcagcag tcggcggatc
      181 gtggcctgga gcctggcggc ccagcgcggc gaaggcgagt ggtcgctgtt tcatttccgc
      241 ccggccggac cgaatcctga aagagttcca tgaacggatc gaagatcgct ctgtttgccg
      301 cggcagcgct cggtattgcc ggcgccgcgc tcaacctggc gtacttgacc ggcaaggcgc
      361 agcaggccga gaaggtgtcg tacatcggca tccgcaaagg cgcgatcatc cgccgcggcg
      421 accggttctc tgaggaccac ctggtgccga tcgatattcc gaagtcgcac gtgggcaacc
      481 tggagcgggt cgcgattcgc tacgccgatc gcggcacggt cgtgggaatg tcggcggtga
      541 aagattacga gggggacgag ctgctcctgc gcggcgatct ggacacgccg ccggcgacgc
      601 ttgagctcaa atcgaccgag cgggcgatgt ggattccggt tgatacgcgg actttcgtgc
      661 cgtcgctggt taacccgggc gatctcgtct cgttcctcgt gggcgacgcg ccgacgcccg
      721 cgatgccgag cgaagcaccg ggcaacgccg aggacggcga caccacgcaa ccgccgctgc
      781 ccgcggtgcc gctgagcggc aggggcgagc tgatcggccc gttccgcgtt ctctccgtcg
      841 gcaatcggct gggccgcacc gacgtgctga aggcctccgg cgtgccgcag ctccaggaaa
      901 acgtgatcac gatcagcgtc accgtgcaag gcgaccagct ggagcccaaa gcggccaagc
      961 tctggaagat gctccacctc agcggctccc gccaggcggg cgtcatcctg caccccagca
     1021 caaagaatta gactcgtcct tggttgttcg acttttcccg tgaagatctg gttcaacaag
     1081 ctgcacgact cgcgccgctc ggtggttgaa acgaccgccg ccgagatcac gctcggccgc
     1141 gacgccacct gcgacgtcgt gctggccagc ccgctggtct cgcggaggca cgccgtcgtg
     1201 cggatcagcg gcgagcggct ggagctggaa aacaccggcc tcaacagcct gctcgtcggc
     1261 gcggcggaga tcttcggcgg gcagcggatc agcttcggcc tgggcgaacg gctgcgaatc
     1321 tggcccttca cgatcacgtt cgaaaaggag caagccccga cgctttcgcg gaccgagttg
     1381 gaggcccaca tccgggcgca gctcgccgac ctggagctgg aaatccacaa gctgctgctg
     1441 gcccggctgg acctggtcga gatcgaaagc cagcggcaga cgctccgcga gcgggtgctc
     1501 ctgctggaaa accatattga agacatttgc cgcgagctgg gcgtctttca cgagcagaac
     1561 gaggtgctgc tggcagagat tgccggcgta gccctccgcg atctcctgat ccaccagctg
     1621 gtgatggagg ccgactggca agccccgccc gacctggcga tgctcaccag caacgagttc
     1681 gacgtgccgg caacgctcgt tcccgagcgc gagaccgagc tgcacgccct cgtgcaccac
     1741 ctgcggacga agctcgacct ggccagcgaa aaggacgccg accgccgcat cgagcgcgtt
     1801 gaaaaggggg cggccgaaac attcccgctg atccgtccgc acctgcacgc cgagctgcgc
     1861 aagtacctga tccaacggac gctgaagaaa gacctcaagg acacggtatt cggcttcgga
     1921 ccgctgcagg atttgctccg cgcgccgaac atcaacgaaa tcatggtcgt ggcccacgac
     1981 cagatttact tcgagcgcaa cggcgtgatc gagctctcgg gccggcggtt cctctcggcc
     2041 gccgtgactg aggcgatcat cgagcggatc gtggcccagg tcggccgccg catcgacaag
     2101 agccagccgc tggtggatgc ccgcctgccc gacggcagcc gcgtgaatgc gatcatcccg
     2161 ccgctggcgg tccgcgggcc atgcctgact atccgcaagt tcggggcccg gcaattcacg
     2221 gtcgaggacc ttatcgaacg ggacacgctg acgcggccgg cggccgaatt cctgcgggcc
     2281 tgcgtcatcg acgagcggaa catcctcgtc agcggcggca ccggtagcgg caagacgacg
     2341 ctgctcaacg tgctgtcgag cttcatcccg cacaagcagc ggatcgtgac cattgaggac
     2401 acgaccgagc tgaagctgct ccaggagcac gtcgtcagcc tggagagcaa gctggccaac
     2461 gtcgaaggga cgggggctta cacgattcgc gacctgctca agaacgccct ccggatgcgg
     2521 cccgaccgga tcatcgtcgg cgagtgtcgc ggacccgagg ccctggacat gctccaagcg
     2581 atgaacaccg gccatgacgg ctcgctgacc ac
//
LOCUS       DASRJB010000248         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3453613, whole genome shotgun sequence.
ACCESSION   DASRJB010000248 DASRJB010000000
VERSION     DASRJB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453613"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1232)
                     /locus_tag="VFB80_06780"
     CDS             complement(60..1232)
                     /locus_tag="VFB80_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33505.1"
                     /translation="MRSAALAAVIAVLCAAGGLLAQEAPLPVPARAVAFSSDGSLLAI
                     GYGTDKIAGGLMIWDGRERKPLLNLRHEWGVSSAAFSPDGKLVAYSNYNRAPQVLEVA
                     GGQVVATLAEDRRGPVAFSPDGQTLATGCMDKSIPLWDVKTRVDRQVLSGAKDRTYGT
                     ILFSQDGTLLLTACGSDGVYLWDLASGKPRHILQHSSFFTRSALFSPDGRWIISGGWD
                     GTTRIWDAASGEARARLDTNSVDALSYAADSRLLAICANGKEVQLFELPLGEPSADQL
                     GQLRAILTRLDDDRIETRDLASAELVKLGFAVDAELKQAATDSPSAEVRIRARRARQA
                     ILTVPPGGISGHEGEVTAAVFSPSGKLLASCSDDGTVRLWDVATRTELARIVPGAR"
     gene            complement(1229..2104)
                     /locus_tag="VFB80_06785"
     CDS             complement(1229..2104)
                     /locus_tag="VFB80_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN33506.1"
                     /translation="MDLDSDGLSDLISGSWPGELYFFRRQADGTFGAAEQLQDKSGKP
                     INLGSASTAFAIDFTGDGTLDLVLGNLVGEVYLLPGEPTGPLVFGAAQRLAAGGEPIN
                     VAGGETAPVVADWDGDRLLDLVVGTGHGSVLWFRNAGTAREPKLEAARTLAGESPLGW
                     GGDESRGKHDWGLRVKPCVVDWNGDGRLDLLLGDRCGSFLAKPTQTDAEKDEERQAND
                     QLPQLRRIWAAAFAEFRQLQAAPTVDAVRLAAVRDRLARLKDEIARVQEIQQHFQPGY
                     QAHGFVWLFLRKATP"
     gene            complement(2129..2434)
                     /locus_tag="VFB80_06790"
     CDS             complement(2129..2434)
                     /locus_tag="VFB80_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN33507.1"
                     /translation="MFHGIVMAGLLVAAPGPEVAADLQLPVPIRAGGRPLDVERDGHS
                     APFAGDIDGDGVRDLLVGQYEQGRLRIYRNLGTNAEPRFETHAWLTAGGQMGRVPEG"
     gene            complement(2449..>2792)
                     /locus_tag="VFB80_06795"
     CDS             complement(2449..>2792)
                     /locus_tag="VFB80_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33508.1"
                     /translation="EVKMADGRTLYTTEKQRPVTLWELASGRVVKELLLEDGGGGPVA
                     FSAGGERFAVASAGVKDPKIAIYSTATGERLESIDSQARCLCFAQDGKSLVAGQANST
                     ALVWQLQSGAK"
BASE COUNT          443 a          996 c          908 g          445 t
ORIGIN      
        1 tcggcgaaga acgaagcctg cgcgtgccgg actagcgtga gcgaactcat gcggctgaat
       61 cacctcgcac ctggcacgat gcgggccagc tcggttcgcg tcgccacgtc ccacaggcgg
      121 acagtgccgt cgtcgctgca gctcgccagc agcttgccgc tgggcgagaa gacggcggcc
      181 gtaacttcgc cctcgtgccc gctgatcccg cccggcggca ccgtcagaat cgcctgtcgc
      241 gcgcggcggg cgcgaatccg cacctccgcg ctgggcgagt cggtcgccgc ctgcttgagc
      301 tccgcatcga cggcgaagcc cagcttgacc agttcggcgc tggccaggtc gcgggtctcg
      361 atccggtcat catcgagccg cgtcaggatc gcccggagtt gtccaagctg gtcggcggaa
      421 ggctcgccga ggggcagctc gaaaagctgg acttccttgc cgttggcaca gatcgccagc
      481 agccgcgagt cggcggcgta cgaaagcgcg tccacgctgt tagtgtcgag ccgagcgcgg
      541 gcctcgccgc tggcggcgtc ccagatgcgc gtggtgccgt cccagccgcc actgatgatc
      601 cagcggccgt cgggcgagaa cagggctgag cgcgtgaaaa agctgctgtg ctgtaagatg
      661 tgtcttggtt tcccgctcgc caggtcccat agataaacac cgtcgctgcc gcaggcggta
      721 agcaggagcg tgccgtcctg cgaaaacagg atcgtcccat acgtgcggtc cttcgcaccg
      781 ctgagcacct ggcggtcgac ccgcgtcttc acgtcccaca gcgggatcga cttgtccatg
      841 cagccggtgg ccagcgtctg cccgtcgggc gaaaacgcca ccggcccgcg gcggtcttcc
      901 gccagtgtgg ccacgacttg gccgccggcc acctcgagca cctgcggcgc gcggttgtag
      961 ttcgaatacg ccacgagttt tccgtcgggc gagaaggccg cggacgagac tccccattcg
     1021 tgccgcaaat tcagcagggg ctttcgctcc ctcccgtccc agatcatcaa tccgccggcg
     1081 atcttatcgg tgccgtagcc gatcgccagc agcgaaccgt ccgaggaaaa cgcgaccgct
     1141 cgcgccggaa ccggcagcgg ggcctcttgc gcgagcaagc cgccggcagc acagaggaca
     1201 gcaatgacag cggcgagtgc ggccgacctc atggcgtcgc ctttcgcaaa aagagccaca
     1261 cgaagccgtg cgcctgatat cccggctgaa agtgctgctg gatttcctgc acgcgagcga
     1321 tttcgtcttt caggcgggcc aggcggtcgc gaacggcagc caatcgcacc gcgtccaccg
     1381 tcggggcggc ttgcagctgc cggaactccg caaaggccgc ggcccagatt cgccgcagct
     1441 ggggcagctg gtcgttggcc tggcgttctt cgtctttctc ggcgtcggtc tgcgtgggct
     1501 tcgccaggaa gctgccgcag cggtcaccca gcaagagatc gaggcggcca tcgccgttcc
     1561 aatcgacgac gcacggcttg acccgcaggc cccagtcgtg cttgccccgc gattcgtcgc
     1621 cgccccagcc cagcggactt tcgccggcga gcgtccgcgc cgcctccagc ttgggctcgc
     1681 gcgccgtgcc ggcgttacgg aaccagagga cgctgccgtg gccggtgccg acgaccagat
     1741 cgagcagccg gtcgccgtcc cagtcggcga ccacgggggc cgtttcgccg ccggcgacgt
     1801 tgatcggctc gccgccggct gccaggcgct gcgccgcgcc aaacaccagc ggcccggtcg
     1861 gctcgcccgg cagcagataa acctcgccga cgaggttgcc cagcaccagg tcgagcgtgc
     1921 cgtcgccggt gaagtcgatc gcaaaggccg tcgaggccga gcccaaattg atcggtttgc
     1981 ccgacttgtc ctgcagctgc tcggccgccc caaaagttcc gtcggcctgc cggcggaaga
     2041 aatacagttc gcccggccag gagcccgaaa tcaaatcact gagtccatcg ctgtcgaggt
     2101 ccactaactg tggaacgaag ccgatgcatc agccttcggg cacgcggccc atttgcccgc
     2161 cggcggtcag ccaggcgtgc gtttcaaatc gcggctcggc gttcgttccc aggttgcgat
     2221 aaatccgcag gcgaccctgc tcgtactggc cgacgagcag gtcgcgcacg ccgtcgccgt
     2281 cgatgtcgcc cgcaaacggc gcggagtgcc cgtcccgctc gacgtccagc ggccggccgc
     2341 cggcccgaat cggtacgggc aattgcaaat cggcagccac ttccgggccg ggcgcggcca
     2401 cgagcaggcc tgccatgaca atgccgtgga acattggatc ctcctttgct acttcgcccc
     2461 cgactgaagc tgccacacca gggcggtcga attggcctgc cccgcgacga gcgacttgcc
     2521 gtcctgggcg aagcagaggc accgcgcctg gctgtcgatc gactccagcc gctcgccggt
     2581 ggcggtgctg tagatcgcaa tcttggggtc tttcacgccc gccgatgcga cggcgaaccg
     2641 ttcgccgcct gccgagaacg ccaccggccc gccgccgccg tcttccagca ggagttcctt
     2701 gacgacccgg ccgctggcca gttcccagag cgtgaccggg cgctgcttct cggtcgtgta
     2761 cagcgtccgc ccgtcggcca tcttcacctc ca
//
LOCUS       DASRJB010000249         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2036916, whole genome shotgun sequence.
ACCESSION   DASRJB010000249 DASRJB010000000
VERSION     DASRJB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036916"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFB80_06800"
     CDS             complement(<1..681)
                     /locus_tag="VFB80_06800"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HZN33509.1"
                     /translation="MKPATSSAKPQASGKRKLPDSAAPPAEATPSGGSRLPLAAATGL
                     EQPLIDRLCATTAAVVYFPVRHHSPAAGRLVAELIGNSRPAAVLIEGPSDYNADFREL
                     LLDHELPIAIYSYFQDAAGERRGAYYPFSEYSPEWQAIRRAAELEIPTRFIDLPWVEV
                     ADLDRATHRYADAELRRGRYVRALCDRLGVEDFDDLWDKLIESEERLTLETYLQRVHL
                     LCWHTRLWE"
     gene            complement(656..1783)
                     /locus_tag="VFB80_06805"
     CDS             complement(656..1783)
                     /locus_tag="VFB80_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN33510.1"
                     /translation="MAKKKPAEAEAPANGAAALQRPAAEMLYADELARLTEASADEPK
                     PRGWRLSPRAALSFVLGDAERGIAPKFVGRRAFLERCIVSLATNRGLMLIGEPGTAKS
                     YLSELLAAAISGDSTLTIQGSAGTTEDNIKYSWNYALLVAEGPSERSLVPAPLYLGLK
                     NGQLVRFEEITRCPLEIQDVLLSVLSDRVMTIPELAGENRSLFAQAGFNVIATANTRD
                     RGVNEMSAALKRRFNFETVPSIAEIGEEMALVERETNRLLSRAGVPVAVTPPITELLV
                     TTFRELRTGKTTDGKGMETLSTVLSTAEAVSTACAAGIHAYFYDDGEVRPRHVVQHLV
                     GTVLKDNPEDLTKVRHYFEHVVKKRGSPLWKSFYEARHELG"
     gene            complement(1787..>3790)
                     /locus_tag="VFB80_06810"
     CDS             complement(1787..>3790)
                     /locus_tag="VFB80_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HZN33511.1"
                     /translation="SPDLVASEWRRMKLRMRTPEHVRAFLACTEYDALDCIRDSIIGE
                     TNKEKCQELVEAFTRVSAPEAAAPMLECKLSCKTPSAAREWLDTHVGHAVAGLIEAAG
                     GRGKLAEAAIDYLRGVKRLGHAEAIAAALQKVKNAELAGKVQRDVLDHEEKSFEPLAD
                     KTTPDWLKKALAAVSKVKAHKLPAWAAPANLPPLPVGEKRLSDEQVTTVLNVLAATSI
                     AEKGDPLLAALREHVERHARDEFAWKLFQSWSEDGCPSKEKWAMGAIGHLGGDMSALK
                     LTPLVRVWPGESQHQRAVFGLECLRAIGSDVALMQLAGCAQKLKFKGLKAKAEQFVNE
                     VAQERGMTRAELEDRVVPDCGLDEQGRHEFDFGSRSFAFVLGSDLKAMVKDADGKVRG
                     DLPKPGGKDDAAKSEQAVADWKLMKKQIKEVATIQAGRLEQAMVTGRRWKVDDFVTLL
                     VKHPLMTHLARSLVWGAFDAKGQRLMALRVTEERDFADVKDNALSLAKAATVGVLHPL
                     ELDEAERSAWGEVLSDYEIVAPFPQFGRAIYKLAPGEEKTDDLKRFHEMKLVAPTLVF
                     TLEKLGWIRGTAMDGGCFDEHSKQFPAADVTAVVVYEGVVGMGYIDPNEMLTVKNVQF
                     VGGMRKPSGYGLDTKGVLKLGAVPPVVMSEVLADLQVLKSKAK"
BASE COUNT          567 a         1295 c         1237 g          691 t
ORIGIN      
        1 ctcccacagg cgcgtgtgcc agcagagcaa atggacgcgc tgcagatagg tctccagcgt
       61 gagccgctct tcggattcga tcagcttgtc ccacaggtcg tcgaagtctt caacgcccag
      121 ccggtcgcag agcgcccgga cgtagcgccc gcggcggagc tcggcgtcgg catagcggtg
      181 cgtggcccgg tcgagatcgg cgacttccac ccagggaaga tcgataaacc gtgtcggaat
      241 ctccagctcg gcggcccggc ggatcgcctg ccactcgggc gagtactcgg agaacggata
      301 gtaagccccg cgccgctcgc ccgctgcgtc ctggaaatag ctgtagatcg cgatcggcag
      361 ctcgtggtcc agcagcagct cgcgaaagtc ggcgttgtag tcgctgggcc cttcgatcag
      421 cacggctgcc gggcgtgaat tgccgatcag ctccgcgacc agccgtccgg cggcgggact
      481 gtggtgccgc acgggaaaat agacgaccgc ggcggtggtg gcgcacaggc ggtcgatgag
      541 gggctgttcg agtccggttg ccgcggcgag cgggaggcgt gagcctcccg atggcgtggc
      601 ctcggctggc ggcgcagcgc tatcgggcag cttacgcttc ccgctcgcct ggggcttagc
      661 cgagctcgtg gcgggcttca taaaagctct tccaaagcgg gctgccgcgc tttttgacga
      721 cgtgctcgaa ataatgccgc accttggtca ggtcctccgg gttgtccttc agcacggtcc
      781 ccaccaggtg ctgcaccacg tgccgcggcc gcacttcgcc gtcgtcgtag aaataggcgt
      841 gaatgcccgc ggcgcaggcc gtgctcaccg cctcggctgt gctgagcacc gtcgacagcg
      901 tctccattcc cttgccatcg gtggtcttgc cggtccgcag ctcgcggaac gtcgtgacga
      961 gcagctcggt gatcggcggc gtcacggcca ccggcacgcc ggcccgcgag agcaggcgat
     1021 tggtctcgcg ctcgacgagg gccatctcct cgccgatctc ggcgatcgac ggcaccgtct
     1081 cgaaattgaa ccgccgcttc agggcggcgc tcatctcgtt gacgccgcgg tcgcgcgtgt
     1141 tggcggtagc aatcacgttg aaccccgcct gggcaaagag cgagcggttt tcgccggcca
     1201 gctcggggat cgtcatcacg cggtcgctga gcaccgatag cagcacgtcc tggatttcga
     1261 gcgggcagcg ggtgatctcc tcgaaccgca cgagctggcc gtttttgagc cccagataga
     1321 gcggcgcggg tacaagggac cgctcgctgg gcccttcggc caccagcaag gcatagttcc
     1381 acgaatactt gatgttgtcc tcggtcgtac ccgcgctccc ctggatcgtg agcgtcgagt
     1441 cgccgctgat cgccgcggcc aaaagctcgc tcaggtaact cttggcggtc ccgggctcgc
     1501 caatgagcat cagcccgcgg ttggtggcca gcgagacgat gcaccgctcc agaaatgccc
     1561 gccggccgac gaacttgggc gcaatccccc gttcggcgtc gcccagcacg aacgacagcg
     1621 cggcccgggg cgaaagccgc cagccgcggg gcttgggctc atccgcggat gcttcggtca
     1681 ggcgggccag ctcgtcggca tagagcatct cggccgccgg ccgctgaagt gcggcagctc
     1741 catttgcagg agcttctgcc tcggctggtt tcttcttcgc catcgactac ttggccttgc
     1801 tcttgaggac ctgcagatcg gccagcacct cgctcatcac gaccggcggc acggcgccga
     1861 gcttaaggac gcccttggtg tccaatccgt agcccgaggg cttccgcatt ccaccgacaa
     1921 actgcacgtt cttgacggtc agcatttcgt tggggtcgat gtagcccatg ccgacgacgc
     1981 cctcgtacac gacgaccgcc gtgacatcgg ccgccgggaa ttgtttggag tgctcgtcaa
     2041 agcagccgcc gtccatcgcg gtgccgcgaa tccagccgag cttttccagg gtaaagacga
     2101 gcgtgggagc aaccagcttc atttcgtgga accgcttcag gtcgtcggtc ttctcttcgc
     2161 ccggcgccag cttgtagatc gcgcggccga attgcgggaa gggcgcgacg atctcgtagt
     2221 cgctgagcac ctcgccccag gctgatcgct cggcttcgtc gagctccagc ggatgcagga
     2281 cgcccacggt ggccgctttg gcaagcgaga gggcgttgtc cttgacgtcg gcaaagtccc
     2341 gctcttcggt gacccgcagc gccattagcc gctggccctt ggcgtcgaac gcgccccaga
     2401 ccagcgagcg ggccaggtgc gtcatcagcg gatgcttgac gagcagcgtg acgaagtcgt
     2461 ccaccttcca gcggcggccg gtgaccatcg cctgctccag gcggccggcc tgaatggtgg
     2521 cgacctcctt gatctgcttc ttcatcagct tccagtcggc caccgcctgc tcggatttcg
     2581 cggcgtcgtc tttgccgccc ggcttgggca aatcgccgcg gaccttgccg tccgcgtcct
     2641 tgaccatcgc cttgaggtcg ctgcccagca cgaacgcgaa gctccgcgag ccgaagtcga
     2701 attcgtggcg gccctgctcg tcgaggccgc aatcgggcac gacgcggtcc tccaactcgg
     2761 cccgcgtcat cccgcgctcc tgagcaacct cgttgacgaa ctgctcggcc ttcgccttga
     2821 ggcccttgaa cttgagcttc tgggcgcacc cggccagctg catcagggcc acgtcgctgc
     2881 cgattgcgcg gaggcattcc aggccaaata ccgcccgctg gtgctggctt tcgccgggcc
     2941 agacccgcac cagcggagtg agcttgagcg cggacatgtc gccgcccaag tgtccgatcg
     3001 cgcccatcgc ccacttttcc ttcgaggggc agccgtcttc gctccaggac tggaatagtt
     3061 tccaggcgaa ctcgtcgcgg gcgtggcgct cgacgtgctc ccgcagcgcg gccaggagcg
     3121 gatcgccttt ttccgcgatc gacgtggctg cgagcacgtt cagaaccgtg gtcacctgct
     3181 cgtcgctgag ccgcttttcg cccaccggca gcggcggcag attggccggc gcggcccagg
     3241 cgggcagctt gtgggccttg accttgctca cggcggcgag cgccttcttg agccagtcgg
     3301 gcgttgtctt gtcggcgagc ggctcgaacg acttctcctc gtggtcgagc acgtcgcgct
     3361 ggacctttcc ggccagttcg gcgtttttga ccttctggag ggccgcggcg atcgcctccg
     3421 cgtgccccag ccgcttcacg ccgcggagat aatcgatggc agcttcggcc agcttgccgc
     3481 ggccgccggc cgcttcaatc agcccggcga cggcgtggcc gacgtgcgtg tcgagccact
     3541 cgcgcgctgc gctgggcgtc ttgcaggaga gcttgcactc cagcatcggc gcggccgctt
     3601 ccggcgcgct gacgcgggtg aacgcctcga ccagctcctg gcacttttcc ttgttcgtct
     3661 cgccgatgat cgaatcgcgg atgcagtcga gcgcgtcata ctcggtgcaa gccaggaagg
     3721 cccgcacgtg ctcgggcgtc cgcatccgca gcttcatccg ccgccattcg gaggccacca
     3781 ggtcgggcga
//
LOCUS       DASRJB010000250        10348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_71072, whole genome shotgun sequence.
ACCESSION   DASRJB010000250 DASRJB010000000
VERSION     DASRJB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71072"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFB80_06815"
     CDS             <1..1131
                     /locus_tag="VFB80_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33512.1"
                     /translation="INIDVGNGNDRVEIGANVAIDAWISGGKGNDDLRGGAGHDQVFG
                     DDGNDKVSGGDGNDLVDAGAGQDQANGGMGDDHVLGGAGHDKLTGGWGNDILEGGAGK
                     DHLAGNDGDDQLLGGDADDKLTGGLGNDLLDGGGGHDHLSGEGGDDQLHGGDGKDQIK
                     GGWGNDELWGDAGHDHLWGDAGNDWLYGGDGNDKLKGGEGDDNLKGEAGDDHLDGGAG
                     SNQLDGDAGKNKLIGGVEFDFDAPPPPPPPPPPPPPPPPPPPPEPSFQPLLAGLNGAL
                     VSGEVHYEQQMTELGMETFLQIYIYGAASGLDLLAATGTIELGHVLTDENGFGELRLS
                     SFGGIEGGNPLPAGFAPMAGDLVTFVDTAVNSLLIAGLFANA"
     gene            1205..1459
                     /locus_tag="VFB80_06820"
     CDS             1205..1459
                     /locus_tag="VFB80_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33513.1"
                     /translation="MKGPHERLKYDLRRLWECPLCKHRERTPGSVTFRHCLCQMKQAD
                     GRPVVMKLLEDGVQRTCPPIVIPHEPPQPPTGGSDAGDQL"
     gene            complement(1798..4062)
                     /locus_tag="VFB80_06825"
     CDS             complement(1798..4062)
                     /locus_tag="VFB80_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33514.1"
                     /translation="MRAAIDFFQRGITRINARIAILVFVVAAGGVAVWQGFTHMSRNQ
                     PKAEGPTKQIAIGTQPAGGDVVPLGSFLPSTQPLNSAGSAGGTHQLIAYGQQDSPATA
                     PPSTAPPAAAPPANSYLPTNNDSLGGYGGSYGGGTFAAPPATGAPTTEAIPENQYRSG
                     TSSTASISDSPAQAQPLSAAPYGGASNGKALNPTGDGGGQGGSSRRGGAFSGQISDAP
                     PPTDPTAAQSEPASAAAAPYAQPAPLPAAAPPANLLPPAELNSSPYSTASRSASEGLS
                     GRALTAIGGAKLAAATPGERQLEGPQQPAISLEKVSPAEIQVGKAATFELYVRNTGQV
                     PAHQVLVTDHVPAGTQLVDARPQPQQGPDGALAWGLGTLQPGEETQITLQVMPQAEGE
                     IGSTAHVSFSAAATSRSICTRPQLTIEHTSPPKVLIGESFHVEITVSNPGSGPATGVR
                     IEEDVPAGLAHAAGAQLEYEVGTLRPGESKRLQLSLTAKQAGQIQNTIHVKGDANLAA
                     QHSLGLEVVAPQLEVDVEGPKRRTLARQATYTVQIANPGTAAARDVELVAFLPRGMKF
                     VATDAQGQYDAAQHAVFWSLEELPPAKAGAVKLTAIPVEPGDQRLRVEGRAGLGLTAA
                     DEQVVQVEQSAEVVVNVKDSDEVIEVGSETTYEIRVTNTGTKAATNVRIVARMPEGMA
                     AVNGEGPTRAAGDASQITFDPLARLNPQEEVIYKVLVQGRQAGDHIVKVQFSTDEQPT
                     PVTREESTRVYQDR"
     gene            4405..5301
                     /locus_tag="VFB80_06830"
     CDS             4405..5301
                     /locus_tag="VFB80_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN33515.1"
                     /translation="MGRVVLGKADVVRWCLVALLSGEHVLLEDVPGVGKTLVAKALAK
                     SVAGDFCRLQFTPDLLPSDILGSSVFDSAQTKFVFHRGPIFANIVLADEINRAPPRTQ
                     SALLEAMSEGQVSIDGQTLPLPRPFLVIATQNPFEYEGTYSLPESQLDRFLLRISMGY
                     PGREDERRVLTTHRQGEPVHELAPVVECEQVAAIQEAVRTVAFDDSVSSYLLEIVDRT
                     RKSDELRVGVSTRGALSLYRAAQALALVEGRTFVVPDDVKRLAPLVLSHRVILKGFLR
                     TNQRDAAEAIIRRLIEQTPVPT"
     gene            5420..6685
                     /locus_tag="VFB80_06835"
     CDS             5420..6685
                     /locus_tag="VFB80_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN33516.1"
                     /translation="MPLRRRAWLTREGWYYLAVLAFIVGGAILRNVNLLVVLAGMMIA
                     PLLFNWRLVMASLSGLAARRKLPEQILAGEPLTVEIEVRNSRRWMSSWLLRVEDWVGE
                     GSGFGVQGSGFGSRESGVGSQNTWWERLRWRMARFDPFHRHDTAAAAVIPHVPARGSG
                     LGTYRITLHRRGRYRFGPLRLSSRFPLGLVKAQITLNEYDELIVAPRIGRLLPEWAAL
                     LEAELIGDQRRHPQRGVTDGDYYGLRPWQSGDSLRWIHWRTTAKLGRPIVRQYERRRS
                     RDCALLLDPYLPPRPTERDQGLLELAISLAATAVHDLTSRGQSRLTVALAGHPPKIHC
                     GPSSPLFCQEALTELADLVPRRSYLLSESLTQTLEAAPRGARLIVISPRPADAASLAD
                     STADLPVDPDDFVWIDAGSEQLPGLFVLE"
     gene            complement(6689..7636)
                     /locus_tag="VFB80_06840"
     CDS             complement(6689..7636)
                     /locus_tag="VFB80_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZN33517.1"
                     /translation="MSQPALSDEILGKIHHGDCIAGMNSLPAGSVDLVFADPPFNIGY
                     DYDVYHDAKDYHEYLAWSRQWIGAVHRVLKPDGTFWLAIGDEYAAELKLESQQLGFRV
                     RSWVIWYYTFGVNCKTKFSRSHAHLFHFVKDPQRFTFRAEELENRIPSARLLVYGDRR
                     SNPTGRLPDDTWILRPQDLSGHFTAEEDTWFFSRVAGTFKERAGFHGCQMPEQLLGRI
                     IRFCSHENEIVLDPFSGSATTLAVAKKLSRRFLGFDLSSAYVKQGTRRLSGISVGDPL
                     DGAPEPTMSAPSTANGRKLADKQNGAARRRRLTSKRVAK"
     gene            complement(7729..7938)
                     /locus_tag="VFB80_06845"
     CDS             complement(7729..7938)
                     /locus_tag="VFB80_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33518.1"
                     /translation="MRTEALPAAAAVIAPGLRRERLARRLDALGLATRVPTHIRTRPR
                     RVDVLLDGRPAALLMKTTTPLFARH"
     gene            complement(7928..8290)
                     /locus_tag="VFB80_06850"
     CDS             complement(7928..8290)
                     /locus_tag="VFB80_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33519.1"
                     /translation="MTIARIHCQVLEMPDREDWLPRTDLGQPRDLLPWADPYIAQLIH
                     QLEDRFDVEDSLAEPCDPFAPDDEDWLLAGDDWEDAFIPRSLDAPRRRWHPPVYGGFP
                     LLEDTITELAEDADADAN"
     gene            complement(8588..9985)
                     /locus_tag="VFB80_06855"
     CDS             complement(8588..9985)
                     /locus_tag="VFB80_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HZN33520.1"
                     /translation="MRIANGAGFLGDWLDAPRRLVERAEVDYLTIEHLAELTMSILAR
                     LREKDPSSGYAEDFLVILKSLTPALQQQSQLKIIANSGGMNPPACVRAASEILVAANL
                     GDTPLGCVTGDDLLPRLPELMAAGCEFKNLDTGQPLAEILSPAQSPQRPAPLVSANAY
                     LGARPIADALAGGARIVVTGRVADASLTVGPAVHHFGWKWDEWNKLAGASVAGHLIEC
                     GAQATGGYSALWEQYDLADVGYPIAEIDAGGEAVITKPAGSGGAVSRRTVVEQLVYEI
                     GDPRHYLTPDVDCDFTTVTVAETGPDRVAVRGATGRPAPDSYKVSLAYRDGWMASGTL
                     LVFGPDCREKAERCGSIILERCRLGGFELARTQVELLGFGGGVPGAWFWRKYQAPGEL
                     VLRVSAHDSRREAIECFAQQFAPLITSGPAGLAGYASGRPQVRPVFAYWPTLVPRSLA
                     LPKVEVRSARDWAAH"
     gene            10109..>10348
                     /locus_tag="VFB80_06860"
     CDS             10109..>10348
                     /locus_tag="VFB80_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33521.1"
                     /translation="MARLSRTRRKSSSPAAVIRTDAGAEIPVPGGPVLQEFAIRWAYL
                     IRNRTHWNKTKWAAMRSQLQRQVKSVGFRQADFRKL"
BASE COUNT         1626 a         3383 c         3607 g         1732 t
ORIGIN      
        1 atcaacatcg acgtcggcaa cggcaacgac cgggtcgaga tcggcgcgaa tgtagcgatc
       61 gacgcctgga tcagcggcgg caagggcaac gacgacctcc gcggcggcgc cggccacgac
      121 caggtgttcg gcgacgacgg caacgacaag gtcagcggcg gcgacggcaa cgacctcgtt
      181 gacgccggcg ctgggcagga ccaggccaac ggcggcatgg gcgacgatca cgtcctcggc
      241 ggcgcgggcc acgataagct gaccggtggc tggggcaacg acatcctgga aggcggcgcg
      301 ggtaaggacc acctggccgg caacgacggc gacgaccagt tgctcggcgg cgacgccgac
      361 gacaagctca ccggcggcct gggcaacgac ctgctcgacg gcggcggcgg acacgaccac
      421 ctcagcggtg aggggggcga tgaccagctg cacggcggcg acggcaagga ccagatcaaa
      481 ggcggctggg gcaacgacga gctgtggggc gacgccggcc acgatcactt gtggggtgac
      541 gccggcaacg actggctcta cggcggcgac ggcaacgaca aactgaaggg cggcgaaggg
      601 gacgacaacc tcaagggcga agcgggcgac gatcatcttg acggcggcgc tggcagcaac
      661 cagctcgacg gcgacgccgg caagaacaag ctgattggcg gcgtcgaatt cgacttcgac
      721 gcgcctccgc ctcctcctcc accgcccccg cctcctcccc cgcctcctcc tcctccgccg
      781 ccagagccga gcttccagcc gctcctggct ggcctcaacg gcgccctagt gtcgggtgag
      841 gtgcactatg agcagcaaat gaccgaattg ggtatggaga ccttcctgca gatctacatc
      901 tacggcgccg cgtcgggatt ggacctgctg gctgcaaccg gcacgatcga actcggccac
      961 gtcctgaccg acgaaaacgg attcggcgag ctgcggctgt cgagcttcgg tggcatcgag
     1021 ggcggcaatc cgctgccggc gggcttcgcc cccatggcgg gagacttggt cacgttcgtc
     1081 gacaccgccg tcaactcgct cctcatcgcc ggcctgtttg ccaacgccta gctgcaaggt
     1141 tcagccgcac caaccatcag ccgggaccaa ccgcggtccc ggctgacgcg ttatgatgtg
     1201 gaccatgaag ggtccgcacg aacggctgaa atacgacctc cgccggttgt gggaatgtcc
     1261 gctctgcaaa caccgcgagc ggacgcccgg cagcgtgacc ttccggcact gcctgtgcca
     1321 gatgaagcaa gccgacggcc ggccggtcgt catgaagctg ctcgaagacg gcgtgcagcg
     1381 aacctgcccg cccatcgtca tcccgcacga gccaccccag ccgccgaccg gcggcagcga
     1441 cgcgggcgac caattgtgaa cattggtacg ctatttgcag tatagttcct tttggcctag
     1501 cggcctgcca ttttggccgc gctggccctg ctctgtcgca atttggttgt gaggtctagg
     1561 gcaaaatcgg ccccgttggc gatcggcctc ttatataacg gaaactcccc gccgggccct
     1621 caaaggctta tgatcctaag gcggctagtt ttccggcggc gcgtccgaag tgtatctata
     1681 ccaagccttt gtgatgcacg tcgcggcgca gtggggcgag tgagaatctt cgcccgaagc
     1741 agttttcgtt atatcgccgg cagtcgctta agggcgaccg atcaccaact cgctggccta
     1801 ccgatcctga tagacccgcg tgctctcctc gcgggtgacg ggcgtgggtt gctcgtcggt
     1861 ggagaactgg accttcacga tgtggtcgcc ggcctgccgg ccctgaacga gcaccttgta
     1921 gatgacctct tcctgcggat tgaggcgggc cagcgggtcg aacgtgatct ggctggcgtc
     1981 gccggcggcc cgcgtcgggc cttccccgtt gaccgccgcc atgccctcgg gcatccgggc
     2041 gacgatccgc acgttcgtcg cggccttcgt gcccgtgttc gtgacccgaa tttcgtacgt
     2101 cgtctcgctg ccgacttcga tgacttcgtc cgagtccttg acgttgacga ccacctcggc
     2161 cgattgctcg acctgcacca cctgctcgtc ggccgcggtc aggccaagcc cggctcgccc
     2221 ctcgacccgc agccgctggt cgcccggctc gacggggatc gccgtgagct tgaccgcgcc
     2281 ggccttggcg gggggcagct cttccaggct ccagaagacg gcgtgctggg ccgcgtcgta
     2341 ctggccctgg gcgtcggtcg cgacgaactt catgccccgc ggcaggaacg ccaccagctc
     2401 cacgtcgcgg gccgccgccg tgccgggatt ggcgatctgc acggtgtagg tcgcctgccg
     2461 ggccagcgtg cggcgcttcg gtccttcgac atcgacctcc agctgcggcg cgacgacttc
     2521 cagccccagc gagtgctggg ccgccaggtt ggcgtcccct ttgacatgaa ttgtgttctg
     2581 aatctgcccg gcctgcttcg cggtcagcga gagctgcagc cgcttcgact cgcccggccg
     2641 cagcgtgccg acttcgtatt cgagctgcgc cccggccgcg tgggccaggc ccgcgggcac
     2701 gtcctcctcg atccgcacgc cggttgccgg accgctgccg ggattgctga ccgtgatctc
     2761 gacgtggaac gattcgccga tcagcacctt cggcggcgag gtgtgctcga tcgtgagctg
     2821 cggacgcgtg cagatgctcc ggctggtggc cgccgcggag aagctgacgt gggccgtgct
     2881 gccgatttcg ccttcggcct gcggcatgac ttgcagcgtg atctgggtct cctcgccggg
     2941 ctgcagcgtg cccaggcccc aggccagcgc gccgtccggt ccctgctgcg gctgcggccg
     3001 ggcatcgacg agctgcgtgc ctgccgggac gtgatcggtc accagcacct ggtgggccgg
     3061 gacctggccg gtattgcgga cgtagagctc aaacgtcgcc gccttgccga cttgaatctc
     3121 ggccggcgag accttttcga gcgagatcgc cggctgctgc ggcccttcga gctggcgctc
     3181 gccgggcgtc gcggctgcga gcttggcgcc gccaatggcg gtcagcgctc gtccggagag
     3241 tccctcgctt gcgctgcggg atgctgtgct gtaggggctg gagttcagct cggctggcgg
     3301 cagcaggttg gccggtggcg cggctgctgg cagcggagcg ggctgggcgt agggcgcagc
     3361 cgcggcggac gccggctcac tttgggcagc cgttgggtcc gtcggcggtg gcgcgtcgct
     3421 gatctggccg ctgaacgcgc cgccgcggcg cgaagagccg ccctgaccgc cgccgtcgcc
     3481 ggtgggattg agcgccttgc cattgcttgc gccgccatac ggcgcggcgc tgagcggctg
     3541 agcctgcgcc gggctgtcgg agatgctggc agtgctgctg gtgccgctgc ggtactggtt
     3601 ttcgggaatt gcctcggtag tgggcgcgcc agtcgccggc ggtgctgcga acgttccgcc
     3661 gccataggac ccgccatatc cgccgagcga gtcattgttc gtcggcaggt acgaattggc
     3721 gggcggagca gccgccggcg gcgccgtgga aggcggcgca gtcgcgggac tgtcctgctg
     3781 cccataggca atgagctggt gcgtgccgcc cgcagaacca gccgaattca gcggctgggt
     3841 gctgggcagg aacgatccca gcggcacgac gtcgcccccg gccggctgcg tgccaatggc
     3901 gatttgcttg gtcgggcctt cggctttcgg ctggttgcgc gacatatggg tgaagccctg
     3961 ccagacggcg acgccgccgg cggccaccac gaacacgaga atcgcgatgc gggcattgat
     4021 ccgcgtgatg ccccgctgga agaaatcgat agctgcccgc atggtcgcgt ccctgcgaaa
     4081 ggcccggaat tcggggaatt tgggtcgggg gtgaatacca gacgctgccc gcggaggaaa
     4141 ggtcatttga tgcggcgaag cccggcaaac cagcgaaact ggccgcttga gggagtttgt
     4201 gtttcgcagc cgcagacaat ggcgagcggc ggcggtagga taatgcggtc tcacgtcttg
     4261 gggtggtgtc ttaaccttgc cgcccaaatg gcagacttgc ctggggcaga tggccgtatg
     4321 gacctctccg ctgccgctgc gaaggacatc gagaggctgc cgcaggagca gttgcagcgg
     4381 ctgatcgccc agctcgaaga gaacatgggc cgcgtggtcc tggggaaggc cgacgtcgtc
     4441 cgctggtgcc tggtcgccct gctgtcgggc gagcacgtgc tgctggaaga cgtgcccggt
     4501 gtcggcaaga cgctggtggc caaggccctg gccaagagcg tcgcggggga cttttgccgg
     4561 ctgcagttca cgccggacct gctcccgagc gacattctgg gcagctcggt cttcgacagc
     4621 gcgcagacaa aattcgtatt tcaccgcggg ccgatcttcg ccaacatcgt gctggccgac
     4681 gagatcaacc gcgcgccgcc gcggacgcag tcggccctgt tggaagcgat gagcgaaggg
     4741 caggtctcga tcgatgggca gacgctgccg ctgccgcggc cgtttctggt catcgcgacg
     4801 cagaacccgt tcgaatacga gggcacctat tcgctgcccg agagccagct cgaccggttc
     4861 ttgcttcgca tttcgatggg ctatcccggc cgcgaggacg agcggcgcgt gctgacgacg
     4921 catcggcagg gcgagccggt gcacgagctg gcgccggtcg tggagtgcga gcaggtcgcg
     4981 gcgatccagg aggcggtgcg gacggtagcg ttcgacgact cggtgagcag ctacttgctg
     5041 gagatcgtcg atcgcacccg caagagcgac gaattgcggg tcggcgtcag cactcgcggc
     5101 gcgctcagcc tgtaccgcgc ggcccaggcg ctggccctgg tcgaagggcg gaccttcgtg
     5161 gtacccgacg acgtcaagcg gctcgcgccg ctggtcctct cgcaccgcgt aatcctcaag
     5221 ggcttcctcc gcaccaacca gcgcgacgcc gccgaggcga tcatccgccg gctcatcgaa
     5281 caaacgccgg tgccgacgta acagtgctag gggctagtgg ctagtgctag tgatcggaaa
     5341 cgaggacacg tgcagcagaa tttgcaagtt gcccgcgaac cagcactagc caccagcact
     5401 agccactagc ccctccgcca tgcccctccg ccgccgtgcc tggctgactc gcgaaggctg
     5461 gtactacctg gcggtgctgg cgttcatcgt cggcggggcg atcctgcgca acgtcaacct
     5521 gctggtcgtg ctggccggca tgatgatcgc cccgctgctc ttcaactggc ggctggtgat
     5581 ggccagcctc agcggcctgg cggcccgccg caagctgccc gagcaaatcc tggccggcga
     5641 gccgctgacc gtcgagatcg aagtccgcaa ctcgcggcgg tggatgagct cgtggctgct
     5701 gcgggtggag gattgggtgg gggagggttc agggttcggg gttcagggtt cagggttcgg
     5761 gagtcgggag tcgggcgtcg ggagtcagaa cacctggtgg gaacggctgc gctggaggat
     5821 ggctcgattc gatccgtttc atcggcacga tacggccgcg gcggcggtga ttccgcatgt
     5881 gccggcgcgg ggaagcgggc tgggcacgta tcgcatcacg ctgcatcgcc gcgggcggta
     5941 ccgcttcggc ccactccggc tttcgtcgcg gtttccgctg ggactggtca aggcgcagat
     6001 cactctgaac gaatacgacg agctgatcgt cgccccgcga atcggccggc tgctgcccga
     6061 gtgggcggcg ctcttggagg ccgaattgat tggcgatcag cggcggcatc cgcaacgcgg
     6121 cgtaaccgac ggcgactact acgggctgcg gccctggcaa agcggcgaca gcctccgctg
     6181 gattcactgg cgcacgaccg ccaagctcgg ccggcccatc gtgcggcaat acgaacgccg
     6241 ccgcagccgc gattgtgcgc tcctgctcga tccctatctg ccgccgcggc caaccgagcg
     6301 cgaccagggg ctgttggagc tggcgatcag cctggcggcg accgccgtgc acgaccttac
     6361 cagccgcggg cagtcgcggc tgacggtcgc cttggcgggg catccgccga agatccactg
     6421 cggcccgtca tcgccgctgt tttgccagga ggcgctcacc gagctcgccg acctcgtgcc
     6481 gcgccgaagc tatttgctgt cggagagcct gacgcagact cttgaggccg ctccccgcgg
     6541 cgcgcggctg atcgtgatca gcccccgccc cgccgacgcc gcttcgctcg ccgactccac
     6601 cgccgacctg ccggtcgatc ccgacgactt cgtctggatc gacgccggca gcgagcagct
     6661 cccggggctg tttgtgctgg agtgaggcct acttggcaac tcgcttggag gtcaatcgcc
     6721 gacggcgggc cgcgccgttt tgcttatcgg ccagctttcg gccgttggcg gtgctgggcg
     6781 cgctcatcgt cggctcggga gcgccgtcga gcggatcgcc tacggaaatg cccgacaatc
     6841 gccgcgtgcc ttgtttgacg taggcgctcg acagatcaaa tcccagaaaa cgccggctga
     6901 gcttcttggc gaccgccagc gtcgtcgcgc tgccgctgaa cggatcgagc acgatttcgt
     6961 tctcgtgcga gcagaagcgg atgatccgtc ccagcagctg ctcgggcatc tggcagccgt
     7021 gaaagccggc tcgctctttg aacgtgccag ccacgcgcga gaagaaccag gtgtcttctt
     7081 cggcggtgaa gtgcccgctg aggtcctgcg gccgcaatat ccaggtgtca tcgggcaggc
     7141 ggccagtcgg attgctgcgc cggtcgccgt acacgagcag ccgagccgag ggaatccggt
     7201 tttccagctc ttcggcgcgg aaggtgaacc gctgggggtc tttgacgaag tggaacagat
     7261 gggcgtggga gcggctgaac ttggttttgc agttcacgcc gaaggtgtag taccagatga
     7321 cccagctgcg gacgcggaag ccgagctgct gcgattcgag cttcagctcg gccgcgtatt
     7381 cgtcgccgat ggccagccag aacgtgccgt cgggcttgag cacgcggtgg accgcgccga
     7441 tccattggcg ggaccaggcc aggtactcgt gatagtcctt ggcgtcgtga tagacgtcgt
     7501 agtcgtagcc gatgttgaac ggcggatcgg cgaagaccag atcgaccgag ccggcgggca
     7561 gcgagttcat ccccgcgatg cagtcgccgt gatggatttt gccgagaatc tcgtccgaga
     7621 gagcgggctg tgacatgggg cgtcgtgccg agcgcggatg ccaaaatggg gcgggtcaga
     7681 tcgcccaatt atagacatcc ccgcgagcgg cgcgccagtg gtcgcggtct agtgcctggc
     7741 aaacagcggc gtcgtggtct tcatcaagag ggccgccggc cgtccatcca gcagcacgtc
     7801 cacgcgccgc ggccgcgtgc ggatgtgggt gggcacgcgc gtggccagcc ccagcgcgtc
     7861 cagtcgcctg gccagccgct cgcgacggag gcccggggcg atgacggcgg ctgcggccgg
     7921 gagagcttca gttcgcatcg gcgtcggcgt cctcggcgag ctcggtgatc gtgtcttcga
     7981 gcagcggaaa gccgccatag acgggcggat gccagcgccg ccgcggagca tcgagcgacc
     8041 ggggaatgaa cgcgtcctcc caatcgtcgc cggccaagag ccagtcctcg tcgtccgggg
     8101 cgaagggatc gcagggctcg gcgagtgagt cctccacgtc gaaccggtct tccagctggt
     8161 ggatcagctg ggcgatgtag ggatcggccc agggaagcag atcgcggggc tgcccgaggt
     8221 cggtgcgggg aagccagtct tcgcggtcgg gcatttccag cacctggcaa tgaatgcggg
     8281 cgatcgtcac ggcagcctcc tgtccgagtg gcggggtgga tttcgagtgg gccatccagc
     8341 cccgcgagga atcccttcga cgctcggcgt gtaaagcaag cccggtgcca agcgggcggc
     8401 aaatcttgcc gcccgcgaag aagcggtggt taagtgtttg ccggacttgg tgttgcgcga
     8461 acgtgcgggg gcggccatct ccggcgatag cagagcgatg cttgcaattc cgagtgcaaa
     8521 tctttcaaag aagtccctcg cttgcgcgtc gggttggtga cgcccactgc ctcgctttcg
     8581 cgtcgggtta gtgtgccgcc cagtcgcggg cgctgcggac ttccaccttg ggcagggcca
     8641 gcgaacgcgg gacgagcgtc ggccaataag cgaagaccgg tcgcacttgc ggccggccgg
     8701 aggcgtaacc ggccagccca gccgggccgc tggtgatcag cggggcgaat tgctgggcaa
     8761 aacactcgat cgcttcccgc cgcgagtcgt gggcgctgac ccgcaggacc agttcgcccg
     8821 gcgcctgata cttgcgccag aaccacgcac ccggaactcc gccgccgaag cccagcaatt
     8881 cgacctgcgt gcgggccaac tcgaagccgc ccagccggca gcgctcgaga atgatcgagc
     8941 cgcaccgctc ggccttttcg cggcagtcgg gaccaaacac gagcagcgtc ccgctcgcca
     9001 tccagccgtc gcggtaggcg agcgagactt tgtagctgtc cggcgctggc cgcccggtcg
     9061 caccgcgcac tgccacgcgg tccggcccgg tttccgcgac cgtgacggtc gtgaagtcgc
     9121 aatcgacgtc gggcgtcagg tagtgccgcg gatcgccgat ctcatacacg agttgctcga
     9181 ctaccgtccg gcggctgacc gccccgccgc tgcccgccgg cttggtgatg accgcttcgc
     9241 cgccggcgtc tatttccgcg atcgggtatc cgacgtcggc caggtcgtac tgctcccaaa
     9301 gggcgctgta gccgccggtg gcctgtgcgc cgcactcgat caggtgcccg gcgacgctag
     9361 cgcccgcgag cttgttccat tcgtcccact tccaaccgaa atggtgcacc gcgggcccga
     9421 cggtgagcga cgcatccgcg acgcggcccg tgacgacgat ccgggcgccg ccggccagcg
     9481 cgtcggcgat ggggcgagcg ccgaggtaag cgttggcgga aacgaggggg gctgggcgct
     9541 gggggctttg agctggggaa aggatttccg ctaacggctg gcccgtgtcg agattcttga
     9601 actcgcagcc ggcggccatc agctcgggca agcgcggcag caggtcgtcg ccggtgacgc
     9661 agccgagcgg cgtgtcgccg agattcgcgg cgacgaggat ttcggaggcg gcccggacgc
     9721 acgcgggcgg gttcatgccg ccggagttgg cgatgatctt gagctgcgat tgctgctgca
     9781 gagcgggcgt gaggctcttc aggatgacga gaaagtcctc ggcgtagccg ctgctggggt
     9841 ccttctcgcg caggcgggcc aggatcgaca tcgtcaattc ggcgaggtgc tcgatcgtca
     9901 ggtaatcgac ctcggctcgc tcgaccagcc ggcggggcgc atccagccag tcgccgagaa
     9961 atcccgcccc gttggcaatc cgcacgaatg gcacgaccgg gctcccagcg gctaacggca
    10021 ttttgacccc gcttcccgga atttggcgct aattccgatt agtgtaaaga ggaaacgacg
    10081 cccgcgttcc tttcgccctg ctgcatcgat ggccaggctc tcacgcaccc gccgcaagag
    10141 ttcgtcgcct gcggccgtca ttcgcaccga cgccggcgcg gaaatccccg tgccgggagg
    10201 tcccgtcctg caggagttcg cgatccgctg ggcctacttg atccgcaacc gcacgcattg
    10261 gaacaagacg aagtgggccg cgatgcgcag ccagctgcag cggcaggtga agtcggtcgg
    10321 atttcgccag gccgactttc ggaaactc
//
LOCUS       DASRJB010000251         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3453852, whole genome shotgun sequence.
ACCESSION   DASRJB010000251 DASRJB010000000
VERSION     DASRJB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453852"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFB80_06865"
     CDS             complement(<1..946)
                     /locus_tag="VFB80_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HZN33522.1"
                     /translation="MPQDKQLREKLLAAASQVAAQLDKSRPLTKDEMEGVARRILAEA
                     GQPEGYTGWMMVILATEFWKDQVAAIPPSRRLFLLPHCLKHAEGCPADYDEFGLECKK
                     CGACSIADFRGLADEMGYKVLVAEGSPIVLKIIVSGYVDAIVGVACLNVLEKAIDKIL
                     LAGIPCMAVPLLSSDCRNTSVDDDWVMRMIKLQATDAAPQTRTYVHLLRAAANLFKPQ
                     ELERIAPKSRKAATSGRGSREGGIRESEAPAEPNGDRPRPLVAALDPISATEAIAYDF
                     LAKGGKYSRPFITLAVYDALTGGKGTGGSGADEVAKFSD"
     gene            complement(1167..>2198)
                     /locus_tag="VFB80_06870"
     CDS             complement(1167..>2198)
                     /locus_tag="VFB80_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN33523.1"
                     /translation="QVLLGTQCGEWLVSCQHRKRHAFTGANPGGFGWSDLSGAVPDAD
                     DTPGAILALCRLNAAWAWGHARNWLKDLQNADGGWPTFCRGWGRLPFDRSGTDLTAHA
                     IRAFRELAEPRGILGEDYFERLYRECVDKGFAYLRRMQRPDGSWSPLWFGNQDHPQED
                     NLVYGTAKVLFAYRDLGLMDSPEAKRGVAWLVANQNADGGWGSGVWGKLRIGDLGLGI
                     SDSEPDLSEIPNPKSQIPNLKSSVEETALAVEALLAACPPTTNHRPPTDPAAAAVSHG
                     LAWLLDRVDAGQHRQPAPIGFYFAKLWYYERLYPLTFTVAALGRACRNFAPAEESDST
                     MKPSATRAT"
BASE COUNT          360 a          723 c          704 g          411 t
ORIGIN      
        1 aatcgctgaa cttcgcgacc tcgtccgcgc cgctgccgcc ggtgcctttg ccgccggtca
       61 gcgcgtcata gaccgccagc gtgatgaacg gccggctgta cttgccgccc ttggcgagga
      121 agtcgtaggc gatggcctcg gtggcggaaa tcggatccaa agccgcgacc aatggtcgcg
      181 gtctatcccc gttcggctcg gcgggagcct cgctctcccg aattccgcct tcccgtgaac
      241 cgcgaccact ggtcgcggct ttgcgagact tgggcgcgat ccgctccagc tcctggggct
      301 tgaacaggtt cgcagcggcc ctgagcaagt gcacgtacgt ccgcgtttgc ggcgcggcgt
      361 cggtcgcttg cagcttgatc atccgcatca cccagtcgtc atcgacgctg gtgttgcggc
      421 agtcgctact gagcagcggc acggccatgc aggggatgcc ggcgagcaaa atcttgtcga
      481 tcgccttctc caacacgttc agacaggcga cgccgacgat cgcatcgacg tagccgctga
      541 cgatgatctt gagcacgatc ggcgagcctt cggccacgag caccttgtag cccatctcgt
      601 cggccagtcc gcggaaatcg gcgatgctgc aggccccgca cttcttgcac tccaggccga
      661 actcgtcgta gtcggccggg cagccctcgg cgtgcttaag acagtgcggc agcaggaaca
      721 gacggcggct gggcgggatt gccgcgacct ggtccttcca gaactcggtg gccaggatga
      781 ccatcatcca gccggtgtag ccctcgggct ggcccgcttc ggccaggatg cggcgggcca
      841 cgccttccat ctcgtccttg gtcagcggcc ggctcttgtc gagctgcgcc gcgacttgcg
      901 acgccgcggc cagcagcttc tcgcggagct gcttgtcctg cggcacgagc ttcaaatgcg
      961 ccgtctgccg ccgcttgctc ttcttgggcg gctcactagc gaactcgggc ggcgcgtctt
     1021 cggagtaagg gacttccctt gcgggctcgc ggtcttgctg ggtgcgcgtg tcgtcgcgcg
     1081 cactgccgag gctgcggctg gtgccgctcg ccgcgtggcc tgtgcggctc agcgtgtcgc
     1141 tcaaggctgc atcgtccaag gtgtcctcac gtggcgcgag tcgcggaggg cttcatcgtc
     1201 gaatcggatt cttcggcggg agcgaaattt cggcaggcgc ggcccaaggc ggccacagta
     1261 aacgtcagtg gatagagcct ctcataatac cacaacttgg cgaagtaaaa accaatcggg
     1321 gccggctgcc ggtgctggcc ggcatccacc cggtcgagga gccaggcgag gccatgggag
     1381 accgccgcgg cggcggggtc ggtcggtggt cggtggttgg tggttggtgg gcaggcggcg
     1441 aggagggctt cgacggcgag ggcggtttct tcgacggagg atttcagatt tgggatttgg
     1501 gatttgggat ttgggatttc ggacagatcg ggttcagagt ccgaaatccc caatcccaaa
     1561 tccccaatcc tcaatttccc ccacaccccg ctcccccatc ctccatccgc gttctggttc
     1621 gccaccagcc acgccacgcc gcgcttcgcc tcgggcgaat ccatcagccc caagtcgcga
     1681 tacgcgaaca gcaccttcgc cgtgccatag accaggttgt cttcctgcgg atgatcctga
     1741 ttgccgaacc aaagcggcga ccaggagccg tcggggcgtt gcattcgtct taagtacgca
     1801 aagcctttat cgacgcactc ccgatacagc cgctcgaagt aatcctcgcc gagtatcccc
     1861 ctgggctcgg cgagttcgcg aaaggctcga atcgcgtgcg cggtcagatc agttccgctg
     1921 cggtcaaagg gcaggcggcc ccagccgcgg cagaatgtgg gccatccgcc gtcggcgttc
     1981 tgcagatcct tgagccaatt gcgagcatgg ccccaagccc aggccgcgtt caagcggcac
     2041 agggccagaa ttgctccagg cgtgtcgtcc gcatccggta cggcgcccga tagatcgctc
     2101 caaccgaatc caccaggatt tgcgccggtg aaagcgtgac gcttgcggtg ctgacacgag
     2161 accagccact ccccgcactg agtcccaagc agcacttg
//
LOCUS       DASRJB010000252         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2609351, whole genome shotgun sequence.
ACCESSION   DASRJB010000252 DASRJB010000000
VERSION     DASRJB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609351"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..>2278
                     /locus_tag="VFB80_06875"
     CDS             58..>2278
                     /locus_tag="VFB80_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN33524.1"
                     /translation="MLRPRELGLIAVLWLAAPALAEGAVPPLVASPDRVELAGSFSRV
                     QLIVARREGEDLTSHAKFASSNESVVRVNASGQVVAVSDGEANVDISVGDEQLSVPVK
                     VSGVSGQPAIRFSEQITPILTKAGCAMAACHAAQHGQGGFKLSVFASEPDKDRAAMVR
                     DAQQRRADFVEPERSLLLLKPTMQVPHGGGKRLEKGSVDYQILLAWLKSGAPGPQKDD
                     DRAIELAVTPTQRIGPKGLTQQLRVEARYASGAVRDVTAWTRFDSLDDGVVGVTSSGV
                     VTAAGQGQAAVMVRFEGLAATSLFVIPSAQMAELAEWHSNNFIDELAAAKFRELGIEP
                     SGLCSDATFVRRAFLDAIGTLPSVDETKAFLADPDPAKRQKLIDRLLGLTGNPALDTY
                     NDAYAAWWALKWSDLLRNNSQDLGEQGMWAMHNWIRESLRINKPFDKFVRELITARGS
                     IYTTGPANYYRVHKDAPMLAEATAQLFLGVRIECAKCHHHPFEKYSQDDYYGLAAFFA
                     RTGTKNSEEFGLFGREQAVIVRDTGDVRHPRTNQVAQPKPLDGPAADHPLDRRIPLAD
                     WLTSKDNAYFSRNVVNRYVGYLLGRGLVDPVDDLRSTNPPSNPPLMDALAKRFTDSSY
                     DLKQLIRTIMNSRLYQLDSQPTAANAGDTKFYSHYQVKRLPAEALLDAIDSVTQMQTK
                     YRNLPLGTRAIELPDAEYPDYFLNTFAKPRRVSVCECERAPDPNLAQALHTLNGDLLA
                     "
BASE COUNT          405 a          755 c          749 g          369 t
ORIGIN      
        1 tgggctcgcg gagttgcctc cgcggtccat cccgccgcta caccgaaagg ttgagtcatg
       61 ctgcgcccca gggagcttgg gctgatcgcc gtcttgtggc tggctgctcc ggcattggcg
      121 gaaggcgccg tgcctccgct ggtggcgagc ccggatcgcg tcgagctggc gggcagtttt
      181 tcgcgggtgc aattgatcgt ggctcgacgc gaaggcgagg atctgaccag ccacgcgaaa
      241 ttcgcaagtt cgaatgagag cgtcgtccgc gtcaatgcgt cgggccaggt cgtggcggtg
      301 agcgacggcg aggcgaacgt cgacatatcc gtcggcgatg agcagctgag cgtgcccgtc
      361 aaggtaagcg gcgtctcagg gcagccggcc atccgttttt ccgagcagat caccccgatc
      421 ctcacgaagg cgggctgtgc gatggcggcc tgccatgcgg cccagcacgg gcagggcggt
      481 ttcaagctgt cggtctttgc ttccgagccc gacaaggacc gcgcggcgat ggtccgtgat
      541 gcccagcagc ggcgggccga cttcgtcgag ccggagcgga gcctgctgct gctcaagccg
      601 acgatgcaag ttccccacgg cggcggcaag cggctggaga aggggtcggt cgattaccag
      661 attctcctcg cgtggcttaa gagcggcgct cccggaccgc agaaggacga cgaccgcgcc
      721 atcgaactcg cggtcacgcc gacgcagcgc atcgggccga agggactgac gcagcagctg
      781 cgcgtggaag ccaggtacgc cagcggcgct gttcgcgacg tgacggcctg gacccgcttc
      841 gattcgctcg acgacggcgt cgtcggcgtg acctcaagcg gcgtcgtgac agcagcgggc
      901 cagggccagg ccgccgtcat ggtccgcttc gaagggctcg cggcgacgtc gctgtttgtg
      961 atcccctcgg cccagatggc cgagctggcc gagtggcaca gcaacaactt cattgacgag
     1021 ttggcggcgg ccaagtttcg cgagctgggc atcgagccgt ctggcctgtg cagcgacgcc
     1081 acgttcgtgc ggcgggcctt cctcgatgcg atcggcacgc taccgagcgt ggacgagacc
     1141 aaggcctttc tggccgatcc cgatccggcc aagcggcaaa agctaatcga tcggctgctc
     1201 ggcctcaccg gcaatccggc cctcgacacc tacaacgatg cctacgccgc ctggtgggcg
     1261 ctgaagtggt ccgacctgct caggaacaac tcgcaggacc tgggcgagca gggcatgtgg
     1321 gccatgcaca actggattcg cgaatcgctg cggatcaata agccgttcga caagtttgtc
     1381 cgcgagttga tcaccgcccg cggctcgatc tatacgaccg ggcccgccaa ctactaccgc
     1441 gtgcacaagg acgcgccgat gctggccgag gcgacggcgc agctatttct cggcgtgcgg
     1501 atcgagtgcg ccaagtgcca tcatcatccg ttcgagaagt acagccagga cgattactac
     1561 ggcctggcgg cgttttttgc ccgcaccggc accaagaaca gcgaggagtt cggcctgttc
     1621 ggccgcgagc aggcggtgat cgtgcgcgat acgggcgacg tgcggcatcc gcggaccaac
     1681 caggtcgctc agcccaagcc gctggacggc ccagccgccg atcatccgct cgaccgccgc
     1741 atcccgctgg ccgactggct gacgagcaag gacaacgcct atttctcgcg aaacgtcgtc
     1801 aaccgctacg tcggctatct gctcggccgc ggtctggtcg atcccgtcga tgacctccgc
     1861 tcgacgaacc cgccgagcaa tccgccgctc atggacgcgc tggcaaagcg tttcaccgac
     1921 agcagctacg acctgaagca gctgatccgc acgatcatga acagccggct gtatcagctc
     1981 gattcgcagc caacggccgc caacgccggc gacacgaagt tctacagcca ttaccaggtg
     2041 aagcggctgc cggccgaggc gctgctggat gctatcgaca gcgtgacgca gatgcagacc
     2101 aagtaccgca acctgccgct gggcacgcgg gcgatcgagc tgcccgacgc cgagtatccc
     2161 gactatttcc tgaacacgtt tgccaagcct cggcgggtca gcgtctgcga atgcgagcga
     2221 gcgcccgatc ccaacctggc gcaggccctg cacaccctca atggcgacct gctcgcgc
//
LOCUS       DASRJB010000253         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2609737, whole genome shotgun sequence.
ACCESSION   DASRJB010000253 DASRJB010000000
VERSION     DASRJB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609737"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFB80_06880"
     CDS             <1..747
                     /locus_tag="VFB80_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN33525.1"
                     /translation="YGSRQDVEAGRCTALKGALRADGRAAYKYVESAGFVQRAAKGFA
                     DMRQALGPDIDLGLDFHGAVEPQTAMLLIKAIEPYQPYFVEEPVQCQNIGVMADIAAK
                     THIPIATGERVFTKWSFREVLEQKAATIIQPDVCYAGGITELRLIAGIAETFYVPLAP
                     HNPNGPCSLAASCQIAAAIPNFLIQERGTSSHENLLKTPFKAEKGYLPVTTAPGLGIE
                     IDEDKFMAQVGEPQPYQRTYDRDDGSVVDW"
     gene            complement(794..1648)
                     /gene="metF"
                     /locus_tag="VFB80_06885"
     CDS             complement(794..1648)
                     /gene="metF"
                     /locus_tag="VFB80_06885"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZN33526.1"
                     /translation="MFYPVRRFTLSFELFPPKTAAGEASLYENVQQLMSFEPDVITCT
                     YGAGGSTRDKTLEIVQQVRQRFGVRVASHLTCVGSTVEQLQNYLREARARGIENIVAL
                     RGDPPKGETAFKPVEGGLRYASELVSLICREFSKFGVAVAGYPETHQEAASPQADLEN
                     LKRKVEAGGHCVITQLFYSNADFFRFRDRCQEIGIRVPIVPGILPVTNLAQIQRITSL
                     CKACLPDEFVAELSQRDDPEWQFAAGVRQATRQVQELIDAGVPGVHFYVLNKSQATAA
                     VLSGVKRP"
     gene            complement(1742..>3322)
                     /locus_tag="VFB80_06890"
     CDS             complement(1742..>3322)
                     /locus_tag="VFB80_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HZN33527.1"
                     /translation="RHLRGPEAERWSDLFLYHHAEFLRGSQAPDEQFRDFKNHVLHVE
                     ENLWGGARLEARRWYGRTVDSLRRREWPEAAYAAGVLSHYFSDPFMPLHTGQCEEETK
                     IHRALEWSISKSYGRLQQIIELDQGGYPQLETPPREDWLERMIVTGAELAHQHYGAML
                     QHYDLARGVRDPLAGMDQECNNRIAQCLAHAVVGFARVLERALAEAEVEPPLVETTLP
                     GFLAVLGAPLRLIAQHLYDLSERLTIEAVFDEVQRTGKVVKNLPPDDREIRQLHAEEV
                     LRIPLHQLDQLPTKLTGTLNGSGCEERHHRNRLRTGSPLVSSGDSLAWREAQARGQEP
                     GARCQEAAPGGRKRFDVIAPAPIPHSAFRTPHSLPPLHPKSPIPNPKFAFRLSSGSPV
                     VDAPSIGPKVAARLEKAGIITVGDLLAAESEGLAGAMRVSHVTAETIHQWQREATLVC
                     RVSELRGHEAQILVASGITDPDELAAAEAQQLLEKITPFVASSEGQRLLRNQQPPNLA
                     DVAQWIDRATSARRLQAA"
BASE COUNT          565 a         1061 c         1098 g          598 t
ORIGIN      
        1 tatggcagtc ggcaggacgt cgaggccggt cgctgcacgg ctctcaaggg cgccctgcga
       61 gccgacggcc gagccgcgta caagtacgtc gagagtgccg gcttcgtgca gcgggcggcc
      121 aagggcttcg ccgacatgcg gcaggcactc ggcccagaca tcgacctggg cctcgacttc
      181 cacggcgcgg tcgagcccca aaccgcgatg ctgctgatca aggcgatcga gccgtatcag
      241 ccctacttcg tcgaagagcc ggtgcagtgc cagaacatcg gcgtgatggc cgacatcgcg
      301 gccaagaccc acatcccaat tgccacgggc gagcgggtct tcaccaagtg gagctttcgc
      361 gaggtcctgg agcagaaggc ggcgacgatc atacagcccg atgtctgcta cgccggcggc
      421 atcaccgaat tgcggctgat cgccggcatt gccgagacgt tctacgtccc gctggccccg
      481 cacaatccca atggcccctg ctcgctggcc gccagctgcc agatcgccgc cgcgattccc
      541 aactttctga ttcaagagcg gggcacgtct tcgcacgaga acctcctgaa gactccgttc
      601 aaggccgaga aaggctatct gcccgtcacg accgcgccgg gcctggggat cgaaatcgac
      661 gaggacaaat tcatggccca ggtcggcgag ccccagccct atcagcggac gtacgaccgc
      721 gacgacggca gcgtggtcga ttggtaatca aagccgcgac cattggtcgc ggtatacgat
      781 acggcgtgat gcgttacggt cgcttgacgc cgcttagcac cgccgcggtc gcctgcgact
      841 tgttcagcac gtagaagtga acccccggca cgccggcgtc gatcaattct tgaacctgcc
      901 tggtcgcttg ccgtacgccc gcggcgaact gccattcggg gtcgtcgcgc tgagagagct
      961 cggcgacgaa ctcgtcgggc aggcaggcct tgcaaagcga cgtgatccgc tggatctgag
     1021 cgaggttggt gaccggcaag atgccgggca caatcggcac gcggataccg atttcctggc
     1081 agcggtcgcg aaagcgaaag aagtcggcgt tgctataaaa cagttgcgtg atgacgcagt
     1141 gaccgccggc ctcgaccttg cgctttagat tctccaggtc ggcctgggga ctcgccgctt
     1201 cctggtgcgt ctcgggatag ccggcgacgg cgacgccgaa cttcgaaaac tcgcggcaaa
     1261 tcaggctgac cagctcgctg gcgtaacgca ggccgccctc gacgggcttg aaagctgttt
     1321 cgcccttggg cggatcgccg cggagagcca cgatgttttc gattccgcga gcgcgggcct
     1381 cgcggaggta attctgtagt tgttcgaccg tcgaaccgac gcaggtcaag tgcgaggcga
     1441 cgcgcacgcc gaaccgctgc cgcacctgct gcacgatctc cagcgtcttg tcccgcgtcg
     1501 atccgcctgc gccgtacgtg caggtgatta cgtccggctc aaaactcatg agctgctgca
     1561 cgttctcata gagcgacgct tcgcccgccg cggtcttcgg cgggaacagc tcgaacgaga
     1621 gcgtgaagcg gcggacgggg tagaacatcg gccaatttca gattgcagat ttcagatttc
     1681 agaatgcaga ttggtccaac caatcttagt cgcccttcgc cgtttcgtcg cgggcgggcg
     1741 atcaggccgc ttgcaaccgg cgggcgctcg tggcgcggtc gatccactga gccacatcag
     1801 ccagattcgg cggctgctga ttgcgaagca gccgctggcc ttcgctgctg gcgacgaagg
     1861 gtgtgatctt ctcgagcaat tgctgagctt ctgcggccgc cagctcgtcg gggtcggtga
     1921 tgccgctggc gaccagaatc tgcgcttcgt ggccgcggag ctcgctcacg cggcagacca
     1981 gcgttgcttc gcgctgccac tggtggattg tctcggccgt gacgtggctg actcgcatcg
     2041 cgccggccag gccctctgac tcggctgcaa gcaagtcgcc cacggtgatg atccccgcct
     2101 tttccaaacg cgctgccacc ttcggcccga tcgacggggc atcgaccacg ggactgccgg
     2161 aggaaaggcg gaaggcgaat ttgggattgg ggattgggga tttgggatgg aggggcggga
     2221 gcgaatgcgg agtgcggaat gcggaatgcg gaatgggagc aggagcaatg acgtcgaaac
     2281 gcttgcggcc acctggcgcc gcctcctggc acctggcgcc tggctcctgg cccctggcct
     2341 gtgcctctcg ccacgccagc gagtcgcccg acgaaacgag cggcgagccg gtgcgcagcc
     2401 ggtttcggtg gtgccgttct tcgcagccgc tgccgttgag agtgccggtg agcttcgtgg
     2461 gcagctggtc gagctggtgc agcgggatgc gcaggacttc ctcggcgtgg agctgacgaa
     2521 tttcacggtc gtccggcggc aggttcttca cgactttgcc ggttcgctgg acttcgtcga
     2581 agaccgcctc gatcgtcagt cgctcgctca gatcgtacag gtgctgcgcg atcagccgca
     2641 gcggcgcgcc aagcactgcg aggaaacccg gcagtgtcgt ctcgacgagc ggcggttcga
     2701 cctccgcctc agccagcgcc ctttccagca cccgggcgaa gccgacgacg gcatgggcga
     2761 ggcactgggc aatgcggttg ttgcattcct gatccatgcc ggccagtgga tcgcgcacgc
     2821 cgcgggccag gtcgtagtgc tggagcatgg cgccataatg ctggtgggcc agctccgcgc
     2881 cggtgacgat catcctttcg agccaatctt cgcggggcgg cgtttcgagc tgtggatagc
     2941 ctccctgatc gagctcgatg atctgctgca ggcggccgta cgacttgctg atgctccatt
     3001 ccagcgcccg gtggattttc gtctcttcct cgcattgccc cgtgtgcagc ggcatgaacg
     3061 gatcggagaa gtaatggctc aatacgcccg cggcataggc tgcctctggc cactcgcggc
     3121 ggcgcaggct atcgacggtg cggccgtacc agcggcgggc ttccagacga gcgccgcccc
     3181 acaggttctc ctccacgtgc agcacgtggt tcttgaaatc ccggaactgc tcatccggcg
     3241 cctggctgcc gcgcaggaac tcggcgtggt gatagaggaa caggtcgctc caccgctccg
     3301 cctccgggcc gcggaggtgc cg
//
LOCUS       DASRJB010000254         9419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_634516, whole genome shotgun sequence.
ACCESSION   DASRJB010000254 DASRJB010000000
VERSION     DASRJB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634516"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3130)
                     /locus_tag="VFB80_06895"
     CDS             complement(<1..3130)
                     /locus_tag="VFB80_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33528.1"
                     /translation="MMKAEEIFLAALERTSPTERAAYLDGACAGNAAQRALVEGLLYS
                     HHEAGSFLDAPLFDAAATIDQPLAERTGAQIGPYKLLQQIGEGGFGVVFMAEQLEPVR
                     RKVALKLIKPGMDTRQVIARFEAERQALAVMDHPHIAKVLDAGATESGRPYFVMELVR
                     GVPITHYCDENHLSVRERLGLFASVCQAIQHAHTKGIIHRDIKPTNVLVTRQDAQAVV
                     KVIDFGIAKAMGQQLTDKTLFTDFAQMIGTPLYMSPEQAELSSVDIDTRSDIYSLGVL
                     LYELLTGSTPVSKEQLKQAAFDEIRRIIREVEPPKPSTRISSAEAAPSIAAQRHTEPA
                     KLARLVRGELDWIVMKALEKDRGRRYETANGFAMDVQRYLDDEPVLACPPSAGYRLRK
                     FARKNKTALATVAAVALLVLVAAGSVGWTLNERRERFRRNADEIVRAVDEGRKLLGQR
                     RWPEALALARHGKNVLDYTAGQNALRPSVEALVKDLEMIQRLEEIELRAADMSVGNGI
                     GYARPANEYHPAFQNYGIDVLALEIDAAAEVRKRQIADYLIESLDAWARDEQDPATRA
                     RLRTVVQKADPEGILARRRAALDRGDTAELQRIITDLGALGRVPPATLAILGETLFRS
                     GLRKEAIEFLRAAHVQHPEAFWINQNLASAHARTEPPQWDEALRHYTAALALRPRSHM
                     LYTNIGVALNALGQRADALAAWRKALELEPTYAPALSNIGWLFVKDEQYDEAEAALQK
                     AIAKQPDFAIAHRNLAVLYGKTKRTTEAMASFDEALKFNHSPADVYSARGTIWMIATE
                     FDKAIADFHRAIEHDDKFAEAYHGLGAVAEKQKHYADAVQWYETALRHDLQLVATHYR
                     LGVTYSAWGQQLKFGSPQLATEKFDKAIAWLNDTIRLDEQYAEAWYSLGNASRERGQI
                     QAAITAFERAAALKPASDDAAYQPHYNLGNIYLRLGDYPQAIKAYRAAISATGYALAY
                     TNLGVALGEQGDLAEAEKYAMEALARVKSPDNIRNVAIALDRQGRYAEALAILQRSLE
                     RFEGGEEQRALIE"
     gene            complement(3167..3430)
                     /locus_tag="VFB80_06900"
                     /pseudo
     CDS             complement(3167..3430)
                     /locus_tag="VFB80_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
     gene            complement(3920..3993)
                     /locus_tag="VFB80_06905"
     tRNA            complement(3920..3993)
                     /locus_tag="VFB80_06905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3957..3959),aa:Arg,seq:acg)
     gene            complement(4086..5417)
                     /locus_tag="VFB80_06910"
     CDS             complement(4086..5417)
                     /locus_tag="VFB80_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33529.1"
                     /translation="MTQTPSPNKPTSRRAFLRNSSLLVAGGAIAASAVQIARAAHPFG
                     SDTIKLGLIGCGGRGTGAATQAMNTSGGEVKLIAMADAFSDRLQGAIRGITSSHPNKV
                     DVPKDRQFVGFNAYNELLQTDCDMVVLATPPGFRPLHFEAAVKAGKQIFAEKPVAVDP
                     AGVRKFLAANEIAKQKGLAVAIGLQRRHERKYMATVKALQDGAIGEIILARAYWNGRK
                     PWFKVRQDGQTEMQYQMRNWYNFNWLCGDHIVEQHIHNLDVINWIKNDYPKTAQGQGG
                     CSLREKDFGEIYDHHMVEFTYGDDTVMLSQCRHQPDTWQSVSEYVHGTKGYCDVSGSK
                     IYDLDDKLLDNFSRLGGDGHQQEHHDLFAELRGGKIPQEGEWGAKSTMTAIFGRMATY
                     SGKQLNWDQCLNSDVVVSPVEKYTSFDDSPPTRPDADGWYALPIPGQAKVV"
     gene            5728..5895
                     /locus_tag="VFB80_06915"
     CDS             5728..5895
                     /locus_tag="VFB80_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33530.1"
                     /translation="MTNDAPRQSLLEELDSRQDELLDELDRLNERIERVLVDCVRWHG
                     TAAAARMPAAA"
     gene            6268..6525
                     /locus_tag="VFB80_06920"
     CDS             6268..6525
                     /locus_tag="VFB80_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33531.1"
                     /translation="MVKYLAMMAVVVGSLVLANVAEAGRRHHGCASCGGCAGGVCAMP
                     VGVAPAPVKAAVVAPEGTTVVAATPAPVYTTARRGLFGWRR"
     gene            complement(6638..8803)
                     /locus_tag="VFB80_06925"
     CDS             complement(6638..8803)
                     /locus_tag="VFB80_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HZN33532.1"
                     /translation="MSRTAMVLVAGLAVVGCVVALLPAQESSRRTASKYRTGAEPPPL
                     PTDGGPPSPTESAPTAPDSLLPPNMLPANLQQQLQAASQQVTQEMVSSPATPPAAFAP
                     AAAQRSISDTPQPAASNTAQPAASNNAQPDANNTTQPAVSNNLQPDANNAASPAAETA
                     PPAELAPPPTDSQPAEDGQLRSVLKRSRPPIVETPSVQPSSPPPAPRARPASSGGSSR
                     RVTSTPAAAPRGAAGMGSISNNRSIQDLAVVGRSPGLRVEVAGPPGVTVGKPALYLVR
                     LINESDAVAEEVVLRVALPGWVRASGSQASLGEAALHADPQGGSRVVWSLPEISPRSE
                     TQLKLQLVSSEGDGFDLSVEWTSKPAAVRASIIVKQPQLELSLAGPADMTFGEEKTFT
                     LTVSNPGSGDAERVVVNVSSGRAPPQQIDVGSLPAGMKKDLPLTVVASQPGVMELRAT
                     AAGEGGIEAEAAGKVVVRKAELNVAVQGPPLKFAGTEATYVVTVANTGTAPAENVNLS
                     LALPAGAKFLGGIDGASPAGGGLRWKIASLAAGTERTYEVRTQLASAGLNRLSVQAQS
                     AAAGAVSAQAETQVEAVSELKLVVNDPSGPLPLGEQAVYEIQVMNRGSQAAQKVKIIM
                     QFSDGVEPVAFDGCDARLVPGQVLCQPLVQLGAGEQVTLRVRAKGQRAGTHQFRVEVT
                     SNDAGTRLVSEGTTRFFSESGLSAAAASTVRKTGDGVQR"
     gene            9003..>9419
                     /locus_tag="VFB80_06930"
     CDS             9003..>9419
                     /locus_tag="VFB80_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN33533.1"
                     /translation="MTYFPKRLVVVPVDFSPESLAALDVGLALVDSPEHLHVVHVLID
                     ITPLEAGEVWGVMDPQARIDQTQKLLREKLADAKYNGIHTAVLLGEPAHGIANYAQEK
                     HAELIVIPSHGRTGITRLLIGSVAERVVRLAHCPVLV"
BASE COUNT         1487 a         3041 c         3083 g         1808 t
ORIGIN      
        1 cctcgatcag tgctctttgt tcctctccgc cctcaaatct ctccaaactt cgctgcagaa
       61 ttgcgagggc ctcggcgtat cgcccctggc gatcgagcgc aatggcgacg ttgcggatgt
      121 tgtccgggct cttgacgcgc gccagggcct ccatggcgta cttctccgct tccgcgaggt
      181 cgccctgctc gcccaaggcg acgcccaggt tggtgtaggc caaggcgtaa ccggtggcgc
      241 tgatcgcggc gcggtaggcc ttgatcgcct gtgggtagtc tcccagccga aggtagatgt
      301 tgccgaggtt gtagtgcggc tggtacgccg cgtcgtccga agcaggcttc agggcggcgg
      361 ccctctcaaa ggcggtgatg gcggcctgaa tttgcccgcg ttcgcgcgac gcgtttccca
      421 agctgtacca ggcttccgcg tactgctcgt ccagccggat ggtgtcgttg agccatgcga
      481 ttgccttatc gaatttctcc gttgcgagtt ggggcgagcc gaacttcaac tgctggcccc
      541 acgcggagta cgtgactccc agccggtagt gggtcgccac caattggagg tcgtggcgca
      601 gggcggtctc gtaccactgg acggcatcag cgtagtgctt ctgcttctca gcgaccgcgc
      661 cgaggccgtg gtacgcctcc gcgaacttgt cgtcgtgttc tatcgcccgg tggaagtccg
      721 cgattgcctt gtcaaattct gtcgcgatca tccagattgt gccgcgcgcg ctgtagacgt
      781 cggcgggact atggttgaat tttagcgcct cgtcgaaaga cgccatcgcc tccgtcgtcc
      841 gtttcgtctt cccgtagagg accgccaggt ttcgatgcgc tattgcgaaa tccggctgtt
      901 tggcaatggc cttttggagc gccgcctcgg cctcgtcgta ctgctcgtcc ttcacgaaca
      961 gccaaccgat gttgctgagt gccggtgcgt aagtcggttc gagctccagc gccttcctcc
     1021 aggccgccag ggcgtcggct cgctggccca gggcattgag cgcgaccccg atgttcgtgt
     1081 agagcatgtg gctgcgcggg cgcagagcga gcgccgccgt atagtggcgc agcgcctcgt
     1141 cccactgtgg cggctcggtg cgagcgtggg cgctggcgag gttctggttg atccagaagg
     1201 cttccggatg ctggacgtgg gcggcgcgca ggaactcgat cgcttccttg cgaagtccgc
     1261 tcctgaacag ggtttcgccg agaatggcga gcgtcgcagg gggcacgcgc ccaagtgccc
     1321 ccaggtcggt gatgatcctc tgcaattccg ccgtatcgcc ccggtccagc gccgctcgcc
     1381 gccgcgccag gattccttcc ggatcagcct tctgcaccac tgtccgcagc cgcgcgcgcg
     1441 tcgccgggtc ttgctcatcc cgcgcccagg cgtcgagcga ttcgatcagg taatcggcta
     1501 tctgccgctt acgaacctcc gccgcggcat cgatctccag cgccagtacg tcgatgccgt
     1561 agttctggaa agcggggtgg tactcgttgg cgggacgggc gtacccgatc ccgttcccga
     1621 cgctcatgtc ggctgctcgt aactcaatct cctccagcct ctggatcatt tccaggtctt
     1681 tgaccagcgc ctcgacgctc gggcgcagcg cgttctgacc agcggtataa tcgaggacgt
     1741 tttttccgtg ccgggcgaga gcgagggctt ctggccagcg gcgctgtccg agtagtttcc
     1801 tcccctcgtc cacggcgcgc acgatctcgt ccgcattccg ccggaatcgc tcgcgtcgct
     1861 cattcagcgt ccagccaacg ctgccggcgg ctaccagcac cagcagcgcg accgcggcga
     1921 ccgtagccag ggccgtcttg ttcttccggg cgaacttccg cagccgatac cccgccgacg
     1981 gcgggcaggc cagcaccggc tcgtcgtcca ggtagcgctg cacgtccatc gcgaagccgt
     2041 tagcagtctc atagcggcgg ccgcggtcct tttccagcgc cttcatcacg atccagtcca
     2101 gctcgccgcg caccaggcgc gcgagcttgg ccggttcggt gtgcctttgg gcggcgatgg
     2161 acggggctgc ttccgccgag ctgattcgcg tgctgggttt gggcggctca acttcgcgga
     2221 tgatccggcg gatttcgtcg aacgcggcct gcttgagctg ctctttgctg acgggcgtcg
     2281 agccggtgag cagctcatac agcagcacgc cgagcgaata aatgtcgctg cgcgtgtcga
     2341 tatcgacact ggagagttcc gcctgctcgg ggctcatgta gagcggcgtc ccgatcatct
     2401 gggcaaagtc ggtaaacagc gtcttgtcgg tgagctgctg tcccatcgcc ttggcgatgc
     2461 cgaagtcgat caccttcacg accgcctgag cgtcctgccg cgtgaccagg acgttggtcg
     2521 gcttgatgtc gcggtggatg atccctttgg tatgggcgtg ctggatcgcc tggcaaacgg
     2581 aggcaaacag tcccagcctc tcgcggacgg acagatggtt ctcgtcgcag tagtgcgtga
     2641 tcgggacgcc gcgaacgagc tccatgacga agtaggggcg gccagactcc gtggctcccg
     2701 cgtcgagcac cttggcgatg tgcggatggt ccatcacggc cagcgcctgc cgctcggcct
     2761 cgaaacgggc aatcacttgc cgcgtgtcca tccccggctt aatcagcttg agcgccacct
     2821 tgcggcggac tggttctagc tgttcggcca taaagacaac gccaaaaccc ccttcgccga
     2881 tctgctgtag cagcttatag gggccgatct gcgcgccggt ccgctcggcg agcggctgat
     2941 cgatcgtcgc agccgcgtcg aacagcggcg cgtccaggaa gctgccagcc tcgtggtgcg
     3001 aatacagcag gccctccacc agggccctct gggcggcatt gcccgcacag gcgccatcca
     3061 ggtacgccgc gcgctcggtg ggcgacgttc gctccagcgc cgccaggaag atttcttcgg
     3121 ccttcatcat gttgggctcc tccggtgcgc gtactcattc cactactcaa tgcgccgcct
     3181 ccaggcgggg cgctccacgt ccaaacagca attcgactcg tttaccgcgg ctgcgcggct
     3241 caccccggat gtcgcggggc agccacgccc gcgcgaaggt ccagtagcgg aacaccgtaa
     3301 ctcgcgagat gtcaagtact tccgacgttt gctcgatcgt aaggccggcg aatggggagg
     3361 tcaccaagca gtgcttcaag cgatcctcga gctggagccg ctgtagcgcc tcgtccagcg
     3421 caaccaagtc atcgggatag gcggactcgg gaagacatct acggcgtcaa acgcaatcaa
     3481 cttggctccg ccggaccctc ggtcgtctca ataaacggcg tgatcgcttc acttggcgtg
     3541 acggagttca tgctccatgc aacggggatt agacaggcca atcgccacct gaattacaac
     3601 gggaggtccg ggaaggttgc agtcaggaac gacaacgtgc tggaagattg ctggtattgc
     3661 gttggtctca ggggaaagcc ccagctcgcc gatcctgagc agcacatccg ctcacgattg
     3721 ggtgagcggc tccgttagaa cggtccttac cttgtccgct gctggatcca gtgccccgtt
     3781 ggaaccatgc cgagggctta cgccagctgc cgaatttgcc accaaacgag taggcgacct
     3841 gacctgtacc cactttttga tgggtacagt ttggggccaa caaaaagagc cactcgacgt
     3901 aagtcgttgt ggctcaaggt acacccagca gggctcgaac ctgcaacctt cggttccgta
     3961 gaccgatgct ctatccaatt gagctatggg tgcatagagc gcgcctgctg aagccaattc
     4021 agcgggcacg ctgaagtcaa tcgtaccggg caggccggat cgtgcaaggt gagtcggcgc
     4081 ggcgatcaga cgaccttcgc ctggccgggg atgggcagcg cgtaccagcc gtcggcgtcg
     4141 ggcctggtgg gcggggagtc gtcgaagctg gtgtacttct cgacgggaga aacgacgaca
     4201 tcggagttga ggcactggtc ccagttgagc tgcttgcccg aataggtcgc catgcggccg
     4261 aagattgcag tcatcgtgct cttggcgccc cattcgcctt cctgcggaat cttgccgccc
     4321 cgcaactccg cgaacaggtc gtgatgctcc tgctggtggc cgtcgccgcc gagccgcgag
     4381 aaattgtcga gcagcttgtc gtccaggtca taaatcttgg agccgctgac gtcgcagtag
     4441 cccttcgtcc cgtggacgta ctcgctgacc gactgccagg tgtccggctg atggcggcac
     4501 tggctaagca tcacggtgtc gtcgccgtag gtgaactcga ccatgtggtg gtcgtagatc
     4561 tcgccgaaat ccttttcgcg gagcgagcag ccgccctggc cctgggcggt cttgggatag
     4621 tcgttcttga tccagttgat gacgtcgagg ttgtggatgt gctgctcgac gatgtggtcg
     4681 ccgcagagcc agttgaagtt gtaccagttc cgcatctggt actgcatttc cgtctggccg
     4741 tcctggcgga ccttgaacca gggcttgcgg ccgttccagt acgctcgggc caggatgatc
     4801 tcgccgatcg cgccgtcctg cagggccttg accgtcgcca tgtacttccg ctcgtgccgc
     4861 cgctgcaggc cgatcgccac ggccagcccc ttttgcttgg caatttcgtt cgcggcgagg
     4921 aacttgcgga cgcccgccgg atcgacggcg accggcttct cggcaaagat ctgcttgccg
     4981 gccttcacgg ctgcttcgaa gtgcagcggg cggaagccgg gcggtgtggc caggaccacc
     5041 atgtcgcagt cggtttgcag cagctcgtta taggcgttga acccgacgaa ctgccggtcc
     5101 ttcggcacat cgaccttgtt cggatgcgag ctagtgatgc cgcggatcgc gccttgcagg
     5161 cgatcggaaa aggcgtcggc catcgcgatc agcttgactt cgccgccgct ggtgttcatg
     5221 gcctgggtcg ccgcgcccgt tccgcggccg ccgcagccga tgaggcccag cttgatcgtg
     5281 tcgctgccga acggatgggc cgcgcgggcg atctggaccg cgctggcggc aattgctcca
     5341 ccggcgacca gcagcgagct attccgcaga aaggcgcggc gcgaggtggg cttgttcgga
     5401 gagggcgtct gggtcatcga tcgggctcct gggtcgaaaa tcgccggatg attgcaggga
     5461 ttcagcggca aaaccgcggg cctcggtgca cgtcctacac taacgcgcga tctccaccgt
     5521 tgcaatcaag ccgtgcgcgc acctaacggt cacgtaagtc tggaaatgcc tgctacttga
     5581 caaaaaaggg atgtggccgc accgcttctc ctggccgctg atccggacct gccgcaggcg
     5641 ggctgaaccg agggcgccca ccgggtcgat ccaaggagcg ccaaggttct ctgcccgcct
     5701 tgccctccgc tcgccccgcg tgcgcgcatg accaacgacg ccccgcgtca gtccctgctc
     5761 gaagagcttg actctcgcca ggacgagctg ctggacgagc tcgatcggct gaacgagagg
     5821 atcgaacggg tgctggtcga ttgcgtgcgc tggcacggca cagccgccgc tgcgaggatg
     5881 cctgccgccg cctgaaaccc agtccaggcg gccatgcgcg catattcgaa gatacgattc
     5941 cggcgccgat aacagccata accgcttgct gcggaacggc ttgcgacacg aaccttgctt
     6001 ctgcaattat gcgcatttat gcgcgcttct cgtcaaagaa cgactctcgg cgccttgagg
     6061 ccgagacccg gctgcagaat tgtaaacaga catgaacaag tgtcaatact gcagctgccg
     6121 gcctcttggt cgttaaacca aacgtatttt gcgttattta cgcagaaata ggaagctgcc
     6181 tacttgacgc attcttccca gttaatacat tcacccatcc acgcgcgtgg tttcgtttca
     6241 aagacctgtg ctaaggagtg aatggaaatg gtcaagtatc tcgcgatgat ggcggttgtt
     6301 gttggttcgc tggtgctcgc gaacgtcgcg gaggccggcc gtcgccacca cggctgtgcc
     6361 agctgcggcg gatgcgccgg cggcgtgtgc gcgatgccgg tcggtgtcgc cccggctccg
     6421 gtgaaggctg ccgttgtcgc gcccgaggga acgacggtgg tcgcggcgac cccggctccc
     6481 gtttacacca ctgcccgtcg cggcctgttc ggctggcgtc ggtagtttga tttgctgggc
     6541 aaagaatcgc ccagcgttgg gcttgcgtat gaagggagag cgagggatcg gtctcctttt
     6601 ttcgtttctt ggccggccgc tgaagggctg gccgcgatca acgctgcact ccgtcgccgg
     6661 ttttgcggac ggtgctggct gcggcggcgg agaggcccga ttcggagaag aaccgcgtcg
     6721 tcccttccga gaccagccgc gtaccggcgt cgttcgaagt cacctcgacg cggaactggt
     6781 gtgtgccggc gcgctggccc ttggcccgca cgcggagggt cacctgctcg cccgcgccga
     6841 gctgcaccag cggctggcag agcacctggc ccggcaccag gcgggcgtcg cagccatcaa
     6901 aggccaccgg ctcgacgccg tcggagaact gcatgatgat cttcaccttc tgcgccgcct
     6961 ggctgccgcg gttcatgacc tggatttcat agacggcctg ctcgcccagc ggcagcggac
     7021 ccgacggatc gttgacgacg agcttcagtt cggacacggc ttcgacttgc gtctcggcct
     7081 gcgcgctgac cgcgccggcc gcggcagact gggcctgcac ggaaagccga ttgaggccgg
     7141 ccgaggcgag ttgcgtccgc acttcatagg tccgctcggt gccggccgcc aggctggcga
     7201 tcttccagcg caggccgccg ccggccggcg acgcgccgtc gattccgccc aggaatttgg
     7261 ctcccgcggg cagcgcgagc gagagattca cgttctcggc gggcgcggtg ccggtgttgg
     7321 caacggtgac gacgtaggtc gcttcggtgc cggcaaactt gagcggcggc ccctgaacgg
     7381 cgacgttcaa ttccgccttg cggacgacga ccttgccggc cgcttcggct tcgatgccgc
     7441 cttctcccgc ggcagtggct ctcagctcca tcacgccggg ctgcgaggcg acgacggtca
     7501 gcggtaggtc cttcttcatg cccgcgggca gcgaaccgac gtcgatctgc tgcggcgggg
     7561 cgcgacctga ggatacattc acgaccaccc gctcggcgtc ccccgagcca gggttgctga
     7621 ccgtcagcgt gaacgtcttt tcctcgccga acgtcatgtc cgccgggccg gccagcgaca
     7681 actcgagttg cggctgcttg acgatgatcg atgcccgcac cgcagccggc ttgctcgtcc
     7741 attcgacgct caggtcgaac ccgtcgcctt cgctgctcac gagctgcagc ttgagctgag
     7801 tctcgctgcg cggggagatt tcgggcaggc tccagaccac gcgcgagccg ccttgtggat
     7861 cggcgtgcaa ggctgcctcg ccgagcgagg cctgactgcc gctggcgcgg acccagccag
     7921 gcaaggcaac tcgcaggacg acctcttcgg cgacggcgtc gctctcgttg atcaggcgca
     7981 cgaggtacag ggccggcttg ccgaccgtga ctcccggcgg cccggccact tccactcgca
     8041 atccgggact gcggccgacg accgccagat cctggatcga gcgattgttc gagatcgagc
     8101 ccattcccgc cgcgccgcga ggagcggcgg ccggcgttga ggtcacgcgg cgcgaggatc
     8161 cgccagatga agcgggtcgc gcacgcggcg ccggcggcgg actcgagggc tgcactgagg
     8221 gcgtttcgac gatcggcggc cggctgcgct tgaggaccga gcgcagttgc ccgtcctctg
     8281 ccggctgcga atcggttggc ggtggagcca actccgccgg tggcgccgtc tcagcagcag
     8341 gcgacgctgc attgttggcg tcgggctgca ggttgttgct aacggcgggc tgcgttgtat
     8401 tgttggcgtc gggctgcgcg ttgttgctag cggcgggctg cgctgtgttg ctagcggcgg
     8461 gctggggcgt atcgctaatg ctgcgctgcg cagccgcagg cgcgaacgcc gctggcggcg
     8521 tagctgggga actgaccatt tcctgcgtca cctgctggct ggcggcttgc agttgctgct
     8581 gcaagttcgc cggcagcatg ttcggcggca gcaagctgtc cggggcggtg ggcgctgatt
     8641 cggtcggcga cggcggtcca ccatcggtcg gcaggggcgg cggctccgcg ccggtgcgat
     8701 acttcgacgc cgtgcgccgc gacgattcct gggccggcaa cagcgctacg acgcagccga
     8761 cgaccgccaa tccggcgacc aacaccatcg cagtgcgaga catggcaaac cccacgtgcg
     8821 gagtcaaaac ggagttaccg cgccgctggt tatcggcgcg cgggaccatg ccggttgaat
     8881 cgactttgcg gcctgcggcg ttttgtgagg ctgcgcaaag tttgccgccg ctagcagtca
     8941 aagctgccgc gctgcgtggg cggccatccc ccacacggtt acactgaatg ggaggacttc
     9001 caatgacgta ctttcccaag cggctggtgg tggtgcccgt cgatttctcg cccgagtcgc
     9061 tggcggcgct cgatgtcggg ctggcgctgg tcgattcgcc cgagcacttg cacgtcgtgc
     9121 atgtcctgat cgacatcacg ccgctcgagg ccggcgaggt gtggggagtc atggacccgc
     9181 aggcccgcat cgaccagacg cagaagctgc tgcgcgagaa gctggccgac gccaagtaca
     9241 acggcatcca cactgccgtg ctgctcggcg aaccggccca cggcatcgcc aactacgccc
     9301 aggagaaaca cgccgagctg atcgtcatcc cctcgcacgg ccgcacgggc atcacgcggc
     9361 tcctgatcgg ctcggtcgcc gagcgggtcg tgcggctggc ccactgcccc gtcctggtg
//
LOCUS       DASRJB010000255         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2610782, whole genome shotgun sequence.
ACCESSION   DASRJB010000255 DASRJB010000000
VERSION     DASRJB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610782"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VFB80_06935"
     CDS             <1..1053
                     /locus_tag="VFB80_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN33534.1"
                     /translation="IIERNGAEIRVPGYSTDNYTQWACDYIKGEGRDKAKPWFLWLCY
                     GAIHGPSTPAPRHLGKYAQEPVPPPSDILPPRPQKPAYLDKVQAWHRGPNGEIVAGAT
                     EAKFGDEGARQKTHADWVRQVNECMLALDEGVGRVIQSLRDSGQLENTLVVYTADQGF
                     GMGEHGFRSKLAPYDATFNSPLIVSFPKQLPEGKVCQGPVCGGDLVATFFAYAGLELP
                     WKIHGRDLTAVFKNPELREQPRALLLEEMGEFYGSATHPIPTDEHLYHSGVPRWIAIR
                     YGPYKYIRTLVAGEMEEIYDVEKDPEELTNLALLPEHAKLLVELRGRAVEELRRTEAP
                     FADSMPPTRQMAAARK"
     gene            1236..>2025
                     /locus_tag="VFB80_06940"
     CDS             1236..>2025
                     /locus_tag="VFB80_06940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZN33535.1"
                     /translation="MPSQLNKYSSRITQPKSQGASQAMLYGTGLKPEDMQKAQVGIAS
                     VWYEGNTCNMHLNQLAAEVKQGVDAAGLVGMRFNTIGVSDGISMGTEGMCYSLQSRDL
                     IADSIETVMGGQWYDALIALPGCDKNMPGCLMAMGRLNRPAIMVYGGTIRAGCTPRHP
                     KLDIVSAFQAYGEFISGRIGEEERAEIIRCSIPGAGACGGMYTANTMASAIEALGMSL
                     PYSSSIPAEDPLKIGECIAAGKAILNLLEKDLKPRDIMTRAAFEN"
BASE COUNT          385 a          682 c          649 g          309 t
ORIGIN      
        1 atcatcgagc gtaacggcgc ggagattcgt gtgccgggct actcgaccga caactacacg
       61 cagtgggcct gcgactacat caagggcgag ggacgcgaca aggccaagcc gtggttcctc
      121 tggctttgct acggcgcgat ccacgggcct tcgacgccgg cgccgcggca tctgggcaag
      181 tacgcccagg agccggtgcc gccgccgagc gacatcctgc cgccgcggcc gcagaagccg
      241 gcctacctgg acaaggtcca ggcgtggcac cgcggaccaa acggcgaaat cgtggccggc
      301 gcgacggagg ctaagttcgg cgacgagggc gcacggcaga agacgcacgc cgattgggtg
      361 cggcaagtca acgagtgcat gctcgcactc gacgagggcg tcggccgcgt catccagtcg
      421 cttcgcgaca gcggccagct ggagaacacg ctcgtcgtct acaccgccga ccagggcttc
      481 ggcatgggcg agcacggttt tcgcagcaag ctcgcccctt acgacgcaac cttcaactct
      541 ccgctgatcg tctctttccc caagcagctg cccgagggca aagtctgcca aggtcccgtc
      601 tgcggcggcg atctggtggc gacgttcttc gcctatgccg gcctggagct gccttggaaa
      661 atccacggcc gtgacctgac ggccgtcttc aagaatccgg agcttcgcga acagccgcgg
      721 gcgctcttgc tggaagagat gggcgaattc tacggctccg cgacccatcc gattccgacc
      781 gacgagcacc tctatcacag cggcgtgccg cggtggatcg cgatccgcta tggcccgtac
      841 aagtacatcc gcacgctcgt ggccggcgag atggaagaaa tctacgatgt cgagaaggac
      901 cccgaagagc tgacgaactt ggccctcttg cccgaacacg ccaagctgct cgtcgaactt
      961 cgcggccggg ccgtcgaaga gcttcgccgc acggaagctc cgtttgccga ttcgatgccg
     1021 ccgacgaggc aaatggcggc cgcacgcaag taggtcccgc ctgccgggcg ggacctgaac
     1081 tgccaaatgc gctccctgcg atccgaggtt ccgcccggca ggcggaacct actctgccct
     1141 gcccgccttt ggcagggcaa gctagattgg agtgtccgca cgcgaaccgg ccgcggccgg
     1201 cgcgtgtctc tgcagcctcc cgccacgttc ccgccatgcc cagtcagctc aacaagtaca
     1261 gcagccgtat cacccagccc aagagccagg gggccagcca ggcgatgctc tatgggaccg
     1321 gtctgaaacc cgaggacatg cagaaggccc aggtgggcat tgccagcgtc tggtacgagg
     1381 gcaacacctg caacatgcac ctcaaccagc tcgccgcgga ggtgaagcag ggagtcgatg
     1441 cggcgggcct ggtcggcatg cgtttcaaca cgatcggcgt ctccgacggc atcagcatgg
     1501 gcaccgaggg gatgtgctac tccctccagt cccgagacct gatcgccgat tcgatcgaga
     1561 ccgtcatggg cggccagtgg tacgacgccc tgatcgccct gcccggctgc gacaagaaca
     1621 tgcccggctg cctgatggcg atgggccgcc tcaaccggcc ggcgatcatg gtctatggcg
     1681 gcacgatccg ggccggctgc acgccgcggc acccgaagct cgacatcgtc tcggcgtttc
     1741 aggcctacgg cgaattcatc agcgggcgga tcggcgaaga ggagcgggcc gagatcatcc
     1801 gctgcagcat tcccggcgcg ggggcctgcg gcgggatgta cacggccaac acgatggcca
     1861 gcgccatcga agccctggga atgtcgttgc cctacagctc gtcaatcccg gccgaagatc
     1921 cgctcaagat cggcgagtgt atcgccgcgg gcaaggcgat cctcaatctc ttagagaaag
     1981 acctcaagcc gcgggacatc atgacccgcg cggccttcga aaacg
//
LOCUS       DASRJB010000256         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2328734, whole genome shotgun sequence.
ACCESSION   DASRJB010000256 DASRJB010000000
VERSION     DASRJB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328734"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFB80_06945"
     CDS             complement(<1..1376)
                     /locus_tag="VFB80_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33536.1"
                     /translation="MQRLALAVIVVFCSGTAVAQPPALKKTTPPTAKKSPQTSSRRTA
                     RRVVLPEGTNLGQSRILPPALREKLPLLYRAGNPWASYSVWRHKNQDASWVAGTPWHA
                     WYISWLCTGQPEYLERAKAMELRAAWSADISGGMRNALRQEGIDACVRYEVLSPHLSD
                     EARREWERRLKLMGDTMLAHCRVGDLDECLASLAVWMLLDRDLGTQYMAARQGPGVES
                     SPADVRDYIADVMSPQQVAGGFFDASTAYYMNNAQLWLLAGAALGPDALPGFDNWARQ
                     AAGYFANSVSYDLAIAQASGDMEGDGSETRLLHFRLGVMWSLIALGYDQDGQLASLAA
                     RLTSADGGPPTAPQHLYPAGQYTWAFLFLDPSDLPRADFRRGQAQGLYVVPGAGIVMH
                     HGQQSSCFAHLLTPKRDDHAYVGSWIDAVWRLEGENVLERPGGYSIGSNEWHTHNGLT
                     FKNNGWFW"
     gene            complement(1901..>4522)
                     /gene="ligD"
                     /locus_tag="VFB80_06950"
     CDS             complement(1901..>4522)
                     /gene="ligD"
                     /locus_tag="VFB80_06950"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZN33537.1"
                     /translation="MSLREYRRKRDFQKTPEPAGKARLRHQQRRFVVQKHAARNLHYD
                     FRLELDGVLKSWAVPKGPSAQPKAKSLAVEVEDHPLDYADFEGIIPRGQYGGGTVMVW
                     DRGTWNPEGDAAADLRRGKLNFSLQGKKLHGRWTLVRMAGREDDDGRKNWLLIKRTDE
                     GARSKRGLRAELDRSVISGRSMDEIAADQDSVWQSGRAEPSASAPRPRRSRSRTPRRA
                     KRRAPRVADLPGARPRPLPEKIEPHLATLVAQVPDTDAWLHEMKYDGYRILARIDGSQ
                     VDLLTRRGNNWTDRFPAVAQAVAELGLSAAFLDGEVVALDEQGRTDFQRLQNWMQRTV
                     AGGGPRDDRSLVYFVFDAPYLEGYDLRQAPLADRKAALAKILPAEDQSGPIRYSDHVR
                     GRGAEVIQLACQSGMEGIISKRADSPYAAGRSASWVKTKCLQRQEFVIGGYTQPSGSR
                     TGFGSLLLGYYRKGQLRYAGHVGTGFTRQSLTDLTARLEALRTGRPAFENPPRSTPRQ
                     RATWVEPKLVAEVEFAEWTEDGVLRQPSFKGLREDKPASQITVERPARLRQQETPMKD
                     DAASADESRVAGIALTHPDRVLYPDLKLTKLGLAQFYESIAEWVVPHLANRPLTLLRC
                     PEGLAGSCFFQKHLAEKLPPALRNVSIREKSATRQYVVLDDLPGLIALVQRGALELHP
                     WPAPADDLEHPDRLVIDLDPGEGVPWKAVVEAAREVRDYLAALELTSFVRTTGGKGLH
                     VVVPLAARATWEEHKQFAAALADTLVARVPQRYIASMKKALRGGKIFVDYLRNQRSAT
                     SIASYSSRARAGAPVATPLSWKELSPRLKPERFTVETLPKRLATLSADPWDGFFTLRQ
                     SLTERMLEAAQNVLADS"
BASE COUNT          745 a         1524 c         1463 g          790 t
ORIGIN      
        1 ttccagaacc agccgttgtt tttgaacgtc aggccgttgt gagtgtgcca ctcgttgctg
       61 ccgatcgaat agccgcccgg ccgctccagc acgttctcgc cctccagtcg ccagaccgca
      121 tcgatccacg agcccacata cgcatggtcg tcgcgcttgg gagtgagcag gtgggcgaag
      181 caactggact gctgcccgtg atgcatgacg atccccgcac cgggcacgac atacaacccc
      241 tgagcttgcc cccggcggaa atccgctcgc ggcaggtcgc tgggatcgag aaacaggaac
      301 gcccacgtgt actggcccgc cggatacagg tgctgcggcg cggtcggcgg cccgccgtcg
      361 gcgctggtca gccgcgctgc aaggctcgcc agctgcccat cctggtcgta gcccaacgcg
      421 atcagagacc acatgacgcc cagccggaaa tgcagcagcc gcgtttcact gccgtcgcct
      481 tccatgtcgc ccgatgcctg ggcgatggcc aggtcgtacg aaaccgagtt ggcgaagtag
      541 ccggccgctt gccgggccca gttgtcgaag ccgggcaagg cgtccggtcc cagggccgcg
      601 ccggccagca gccacagctg cgcgttgttc atgtagtacg ccgtgctggc atcaaaaaac
      661 ccgccggcaa cctgttgcgg cgacatcacg tcggcgatgt agtctcgcac gtccgccggc
      721 gaactctcga cgcccgggcc ctggcgggcg gccatgtatt gcgtgcccag gtcgcggtcg
      781 aggagcatcc acaccgccag cgacgccagg cactcgtcca gatcgcctac gcggcaatgc
      841 gcgagcatcg tatcgcccat caatttcagc cgccgctccc actcgcggcg cgcttcgtcg
      901 gataggtgcg ggctgagcac ctcgtaccgc acgcaagcgt cgatcccttc ctgccgcagg
      961 gcgttgcgca tgccgcccga aatgtcggcc gaccaggccg cgcgcagctc catcgccttg
     1021 gcccgctcca gatactccgg ctgcccggtg cacagccagg aaatgtacca ggcgtgccag
     1081 ggagtgccgg ccacccaact ggcgtcctga ttcttgtgcc gccacaccga atagctggcc
     1141 catgggttgc cggcccgata gagcagcggc agcttctcgc gcagcgccgg cgggagtatc
     1201 cgcgactgcc caagattcgt gccctcgggg agcacgaccc tgcgggcagt gcgcctgctg
     1261 gaagtctgcg gagacttttt cgcggtgggc ggcgttgttt tcttgagagc gggcggctgc
     1321 gcgacggccg tgccactaca gaacacgact attactgcca gtgcgagtcg ctgcatcgct
     1381 acgagtcctt tcagccggag attccagcca accagagtgc cgagcgtgcc acagcaaccc
     1441 gtgggccgat cattctacgg cccaatccgt ccctgcaagg ggggcggacc tggggacgaa
     1501 ggcgcgaccc tatgcgcgca ttgcctagag agttatttcc gtcgctgttt ttgccataag
     1561 tcttgttgcc gcaattagtt gcgacgaaac aattgctctg gcgattatgc gcattatgcg
     1621 ctgaccgaat tggctgcccg ccgtgcgcca gtccgctggc aaggcctatg cgcgcatggc
     1681 ggagcctttt gttttcgcca catttctcgt cgtaagtact gtttccacaa tcacttgcgg
     1741 caagagactc gcgccggcga atatgcgcat tatgcgcgca agcaaaaggc ccacccgttg
     1801 ggagcggatt tgaccaaatg aattgtcaat gagcatcacc cgccgcatcc gcggcgaggg
     1861 tccaggccga accgtaatta taacaactgt acacttgagg tcaagaatcg gccaggacgt
     1921 tttgagcggc ctcaagcatt ctctccgtaa gactctggcg aagtgtaaag aagccgtccc
     1981 agggatcggc gctcagggtg gccaggcgtt tgggaagcgt ctccacggtg aatcgctcgg
     2041 gcttgaggcg gggcgagagt tctttccagg atagcggcgt cgcgaccggc gcgcccgcgc
     2101 gggcccgcga tgaataggag gcgatggagg tcgcgctgcg ttgattgcgc agatagtcga
     2161 cgaaaatctt gccgccccgg agggccttct tcatgctggc gatgtagcgc tgcggcacgc
     2221 gggccaccag cgtgtcggcc aaagcggcgg cgaattgctt gtgttcctcc caggtcgcgc
     2281 gggccgccag tggcactacc acgtgcagcc ccttgccgcc agtcgtgcgg acgaagctgg
     2341 tcagctccag cgccgccagg tagtcgcgga cctcccgcgc cgcctcgacg acggccttcc
     2401 acgggacgcc ctcgccggga tcgaggtcga tcaccagccg atcgggatgc tccaggtcgt
     2461 cggccggcgc cggccacgga tgcagctcca gcgcgccgcg ctgcaccagc gcgatcaggc
     2521 ccggcaagtc gtccaagacg acgtactgcc gggtcgcgct cttctcgcgg atgctgacgt
     2581 tgcgaagcgc cggcggcagc ttctccgcca ggtgcttttg aaagaaacac gagccggcca
     2641 ggccctcggg gcagcgcagc agcgtcaacg gccgattcgc caggtgcggc acgacccact
     2701 cggcaatcga ttcatagaac tgggccaggc cgagcttcgt gagcttcaag tccggataga
     2761 gcacgcggtc gggatgcgtc agcgcaattc ccgcgacgcg ggactcgtcg gccgatgccg
     2821 cgtcatcttt catgggcgtc tcctgttgtc gcagtcgcgc gggccgctcc acggtaatct
     2881 gcgacgccgg cttgtcctcg cgaagaccct tgaacgaagg ctgccgcaac acgccgtcct
     2941 cggtccactc cgcaaactcc acctccgcga ccagcttggg ctccacccag gtcgcccgct
     3001 gccgcggcgt cgagcggggc ggattttcaa acgccgggcg gccagtccgc agcgcctcga
     3061 gccgggccgt caggtccgtc agcgattggc gggtgaagcc ggtgccgacg tggcccgcgt
     3121 aacgcagctg gcccttccga taatagccca agagcagcga cccgaaaccg gtgcgcgacc
     3181 ccgacggctg cgtgtagccg ccgatcacga attcctgccg ctgaaggcac ttcgtcttga
     3241 cccagctggc gctgcgcccg gccgcatagg gactgtccgc ccgcttggag atgatgccct
     3301 ccatccccga ttggcaggcc agctggataa cctcggctcc tcgcccgcgg acgtggtcgc
     3361 tgtagcgaat cggcccactt tggtcttcgg ccgggaggat tttggccagg gctgccttgc
     3421 ggtcggccag cggcgcttgc cgcaagtcgt agccctccag atagggcgcg tcgaagacaa
     3481 aatagaccag cgagcggtca tcgcgcggcc cgcccccagc aacagtgcgc tgcatccagt
     3541 tttgcagccg ttggaagtcg gtccggccct gctcgtcgag ggccacgacc tcgccgtcga
     3601 gaaacgccgc ggaaaggccg agctccgcca ccgcctgggc gactgccggg aaccggtcgg
     3661 tccagttgtt tccgcggcgc gtcaagagat cgacctgcga gccgtcgatc cgggcgagaa
     3721 ttcggtagcc gtcgtacttc atctcgtgca gccaggcgtc cgtgtctgga acctgggcga
     3781 cgagcgtggc cagatgcggc tcgatttttt cgggcagcgg ccgcgggcga gcgcccggca
     3841 aatcggccac gcgcggcgct cgccttttgg cccgccgcgg agttcgagaa cgcgagcgcc
     3901 gcggacgagg agcggatgcg gacggctccg cgcggccgct ctgccacacg gaatcctggt
     3961 cggcggcgat ctcgtccatc gagcgaccag agatcacgct gcggtccagc tccgcgcgca
     4021 ggccgcgctt ggatcgcgcg ccctcgtccg tccgcttgat gagcagccag tttttccgcc
     4081 cgtcgtcgtc ctcgcggccg gccatccgca cgagggtcca gcggccatgc agctttttgc
     4141 cttgcagcga gaagttgagc ttgccgcggc gcaaatcggc agccgcgtcc ccttcgggat
     4201 tccaggtgcc gcggtcccag accatgacgg tgccgccgcc gtattggccg cgggggatga
     4261 ttccttcgaa gtcggcgtaa tcgagcgggt gatcctcgac ctcgaccgcc agcgatttcg
     4321 ccttgggctg ggcgctggga cctttgggca cggcccagct cttgagcacg ccgtccaact
     4381 ccaggcggaa gtcgtaatgg agattccggg cagcgtgctt ctgcacgacg aatcgtcgct
     4441 gctggtgccg gagtctggcc tttccagccg gctcgggcgt cttctggaag tctcgtttgc
     4501 ggcgatattc gcggagcgac at
//
LOCUS       DASRJB010000257         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_356499, whole genome shotgun sequence.
ACCESSION   DASRJB010000257 DASRJB010000000
VERSION     DASRJB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356499"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFB80_06955"
     CDS             complement(<1..399)
                     /locus_tag="VFB80_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HZN33538.1"
                     /translation="MTTDDARLAKLKDQLQPIGQQHLVSFWPRLSAAEREQLAQQIEA
                     IDVEEFRELQADFRQRQAAGGDEKSQWAALAARAESPPAMRLDGSGVPFTPDQSRSRG
                     AELLRSGEVGMILVAGGLGTRLGFDQPKGLF"
     gene            627..1592
                     /locus_tag="VFB80_06960"
     CDS             627..1592
                     /locus_tag="VFB80_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33539.1"
                     /translation="MTLWLCSLAFAGLAADAPAEVKLSEESIVRFATAQEGQAVLTTD
                     DAFTRSLSKFDLQSRLKTADAVTLDDWKKSVVQHVRPWQPEEIAAVSESFQRLSKPLG
                     QLRLPLPKSVLLIRTTGDEESNAAYTRGAAIILPARVVKYAQGPLDKLVAHELFHVLS
                     RHDGALRARLYRIIGFEVREPIEFPKSLADRRITNPDAPLVDCTIELTNAEGQAVVGA
                     PILYASPASYNAEKKGSMFGYLTFRLLAVENDGGRWQARLVRGEPVVIDPKKEPAFFE
                     KIGKNTNYVIHPDEILADNFVHLVLGTKDLATPRIVDEMRAALAK"
     gene            complement(1596..>2909)
                     /gene="purH"
                     /locus_tag="VFB80_06965"
     CDS             complement(1596..>2909)
                     /gene="purH"
                     /locus_tag="VFB80_06965"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN33540.1"
                     /translation="PEDLTAAREHGIELLDLVVVNLYPFTQTIAKPGVSAAEAIENID
                     IGGPSLVRAAAKNHAFTAIATGPGQYAEVTAALREGGTTLALRQKLAAAAFAHTAAYD
                     AAIANWFGGQLQGELFPPTLHLALKRQTELRYGENAHQRGAVYADASARGTSLVAARQ
                     LNGKELSYNNLLDLDSALAVVRPLTEPAASVIKHNNPCGAATARTLADAAQKALGGDP
                     QSAFGGVLAFNRPIDAATAEILAAPGLFIEAIVAPDFEPAALQILTTKPKWKANVRLL
                     AAGPLDAPPTAIGLRQIEGGMLVQETDTAADPEAEWKVVTQRSPDDALLADLRFGWAV
                     VRHVKSNAIALAKGTALVGCGAGQMSRVDSVEIAIQKAGPRAKGAILASDAFFPFPDS
                     IHRAAAAGIAAIIQPGGSVRDDEVIAAANEHGIPMIFTGRRHFRH"
BASE COUNT          473 a          982 c          982 g          472 t
ORIGIN      
        1 gaacagcccc ttgggctgat cgaagcccag ccgcgtgcct aggccgcccg cgaccaggat
       61 catgccgacc tcgccgctgc ggagtagctc tgcaccgcgg gagcgcgact ggtccggagt
      121 gaaggggacg ccgctgccgt ccagccgcat cgccggcggc gactcggcgc gggccgccag
      181 cgccgcccac tgcgacttct catcgccgcc cgccgcttgc cgctggcgga agtcggcctg
      241 cagctcgcga aactcctcga cgtcgatcgc ttcgatctgc tgcgccagct gctcgcgctc
      301 ggcggcggac agccgcggcc agaaggacac gaggtgctgc tggccgatcg gctgcagctg
      361 gtccttcagt tttgccaggc gggcgtcgtc cgtggtcatc tagcgtcctt ctggctctca
      421 aaacagccgg ccctgcaccc acaaccaggc caggtagcac aggatcggaa acagtacaaa
      481 taccgcgccg taagtgaaaa tagtggtcca gacgtgccgc ggccagcgag ccatctccgc
      541 cccctgccgg ctgctatccg gcgccctcta ccgactagaa tatcggtcgg tccccattca
      601 gccaaccgcc gccacgaacc cgcaccatga cactctggtt gtgctcgctc gcgttcgccg
      661 gcctggcggc ggatgctccc gccgaagtca agctgagcga ggagtcaatc gtccgcttcg
      721 ccacggccca agaagggcaa gcggtgctga cgacggacga tgccttcacg cggagcctga
      781 gcaagttcga cctccagtcc cgcctgaaga cggcggacgc cgtcacgcta gacgactgga
      841 agaagtccgt cgtgcagcac gtgcgcccct ggcagccgga ggaaatcgcc gccgtcagcg
      901 agtcgttcca gcggctgagc aagccgctcg gccagttgcg gctgccgctg ccgaagtccg
      961 tgctgctgat tcgcaccacg ggcgatgagg agagcaacgc cgcctacacc cgcggcgcgg
     1021 caatcatcct gccggcgcga gtcgtaaaat acgcgcaagg gccgctcgac aagctggtgg
     1081 cccacgagct gttccacgtc ctcagccggc acgacggggc gctgcgggcc cggctgtacc
     1141 gcatcatcgg cttcgaggtt cgcgagccga tcgaatttcc caagtcgctg gccgaccggc
     1201 ggatcaccaa ccccgatgct ccgctggtcg attgcacgat tgagctgacg aacgcggaag
     1261 ggcaggccgt cgtcggcgca ccgatcctgt atgcctcgcc tgcgagctac aacgccgaaa
     1321 agaagggctc gatgttcggg tacctcacct tccggctgct ggcggtcgag aacgatgggg
     1381 gccgctggca ggcgagactc gtccgcggcg agccggtggt catcgacccg aagaaagagc
     1441 cggcgttctt cgagaagatc ggcaagaaca cgaactacgt gattcatccc gatgaaatcc
     1501 tcgcggacaa tttcgtgcac ctggtgctcg gcacgaagga ccttgccacg ccacggatcg
     1561 ttgacgaaat gcgtgcggca ctggcgaagt agcgcttagt gccgaaagtg ccgccggccg
     1621 gtgaagatca ttggaatgcc gtgctcgttg gccgcggcga tgacctcgtc gtcccggacg
     1681 ctgccgcccg gctgaatgat ggcggcgatg ccggcggcgg cggcgcggtg gatggaatcg
     1741 gggaacggaa agaaggcatc ggaagcgagg atcgcgccct tggcccgtgg gccggccttt
     1801 tgaatcgcga tctcgaccga atcgacgcgg ctcatctgac ccgcgccgca gccgacgagc
     1861 gccgtccctt tggccagtgc gatcgcgttg ctcttgacgt gccgcacgac ggcccagccg
     1921 aagcgcaaat cagccagaag ggcgtcgtcg ggcgagcgct gcgtcacgac tttccactcg
     1981 gcttcggggt cggctgcggt gtcggtctcc tggacgagca tgccgccctc gatctgcctc
     2041 aggccgatcg ccgttggcgg tgcatccagc gggccggcgg caagcagccg cacgttggcc
     2101 ttccacttgg gcttggtcgt gagaatctgc agcgcggcgg gttcgaaatc cggggcgacg
     2161 atcgcctcga tgaacagccc gggcgcggcg agaatctcgg cggtcgccgc gtcgatcggg
     2221 cggttgaaag ccaggacgcc gccgaacgcg ctttgcggat cgccgcccag ggccttctgg
     2281 gccgcgtcgg ccagcgtgcg ggctgtggcc gcgccgcagg gattgttgtg cttgatgacc
     2341 gacgctgccg gctccgtcag cggccgcacg acggccagcg cactgtcgag atcgagcagg
     2401 ttgttgtatg acagctcttt gccgttgagc tgccgggccg cgacgaggct cgtgccgcgg
     2461 gcggacgcgt cggcgtaaac cgccccgcgc tgatgggcgt tctcgccata gcgcagctcg
     2521 gtctgccgct tcaaggccag gtgcaaggtc ggcgggaaca gttcgccctg cagctgaccg
     2581 ccgaaccagt tggcaatcgc cgcgtcgtag gccgccgtgt gggcgaacgc agccgcggcc
     2641 agcttctgtc gcagggccag cgtcgtgccg ccttcgcgca gggctgcggt cacctcggcg
     2701 tattgtccgg gcccggtcgc aattgccgtg aacgcgtggt ttttcgcggc ggcccgcacc
     2761 aggctgggcc cgccgatgtc gatgttctcg atcgcctcgg ccgcgctcac gcccggcttc
     2821 gcaatcgtct gggtgaatgg atagaggttg acaacgacca ggtccaagag ctcgatgccg
     2881 tgctcgcggg ccgccgtcaa gtcctccgg
//
LOCUS       DASRJB010000258         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1484701, whole genome shotgun sequence.
ACCESSION   DASRJB010000258 DASRJB010000000
VERSION     DASRJB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484701"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..574)
                     /locus_tag="VFB80_06970"
     CDS             complement(62..574)
                     /locus_tag="VFB80_06970"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HZN33541.1"
                     /translation="MAEKDYLIDLSLFDYEHPVATLDDIRRYNPQRFEMEQLTAVVYV
                     NEADYSCAGYKDVTDKEFWVRGHMPGLPLMPGVVMLESIAQLCSFVTQRYDLLGAGMV
                     GFGGLEEVRFRDPVVPGDRLIVMCRLDKVRRGRIIICDFQGVVQDRIVVEGILKGIPI
                     PVEALQKLKK"
     gene            complement(805..2130)
                     /locus_tag="VFB80_06975"
     CDS             complement(805..2130)
                     /locus_tag="VFB80_06975"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33542.1"
                     /translation="MDRRSFLEASAAATAFASLTSAARAQNRSPSDKISICMMGVRGR
                     GAGVLATFAGLPEVEVRYVCDLDEQVRESRLAQVEQKTGKKPIGLKDFRQALEDKTLD
                     ALVLGTPDHWHALPTIYACQAGKDVYVEKPDGHNILEGRTMVAAAKKYGRVVQLGTQS
                     RSGQHFFSCMDYLKTGALGKVRFAKAWESAKQGSLGKPADSDPPAGFDYDTWLGPAPK
                     KPFNRMRFHGNWRWFFDLGTGDLGNDGVHRLDYCRWALASAAAAQKETLPDLPRAVSA
                     LGGKYYFDDIQEWPDTLMVTYDFAPNYLMTYEMRVWNSYPLHEESEGAAVCGDNGFVV
                     IGNTRWRAFDAKGKQIKEEKGDYNNDVAHAKNFLDCMRSRGKPNADLETIGHPSSLLC
                     HIGNAAWRAGRTLRFDPATYTFAGDADANQYLTRAEYRKPWVLPKLSEL"
     gene            complement(2227..2595)
                     /locus_tag="VFB80_06980"
     CDS             complement(2227..2595)
                     /locus_tag="VFB80_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33543.1"
                     /translation="MRKMCVAAVVVALGWLVVSLTSTAQEDKKVTIKDVMKTAHKGGL
                     MAKVAKGEANADEKKQLVELYTALAANKPPKGDEASWKEKTAALLAAAKEAAEGKEGA
                     GAKLRAAANCMACHSVHKGA"
     gene            complement(2753..>4072)
                     /locus_tag="VFB80_06985"
     CDS             complement(2753..>4072)
                     /locus_tag="VFB80_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33544.1"
                     /translation="TREDLKEAIEDVLAGREVRVATTPVDGCLIEFDALAALPAAVPK
                     EVTYTRHVAPILAKHCQECHRPETSAPFSLLSFKDARDHAETLAEVVRQERMPPAFAS
                     REQTQIVNARRLSDQERALLLAWARSGKLEGDPSELPPPREFASGRWKIGEPDLELKL
                     VTTQEIPAEGIVPYRYVLLPHVFLQDTWVQRVEILPGNKSAVHHANLAYVKVGAPPAS
                     ENFITGYVPGGDAMVLDGGVGFKIPAGSVLALQIHYVTTGMKATDRTSVGLGFAKEPI
                     QKELRHFQGVNRRFEIPPGASHHPVSATRTFAADSTGIGMFAHMHLRGKDMTFRARYP
                     ASGDDPERSEVLLAIPNYSFDWQISYRWAAGQKKFQKGTKYEVTAHFDNSAFNPYNPD
                     PKATVKEGEQTFQEMMYGFLFYTHDDEQLGLEIDPKTGRVIGQSDGR"
BASE COUNT          638 a         1356 c         1296 g          782 t
ORIGIN      
        1 ccaaagaacc atgccgtgca acggagtttt tggttcctcg gttctttggt tctttgagtt
       61 tctacttctt gagcttttgc agcgcttcga ccgggatcgg gatgcccttg aggatgccct
      121 cgacgacgat ccggtcctgc acgacgccct gaaagtcgca gatgatgatc cgcccgcggc
      181 ggaccttgtc gaggcggcac atcacgatca gccgatcgcc cggcacgacg ggatcgcgaa
      241 agcggacttc ctccaagccg ccgaacccga ccattcccgc gccgagcagg tcgtaccttt
      301 gcgtgacgaa gctgcagagc tgagcgatcg attcgagcat cacgacgccg ggcatcagcg
      361 gcagtcccgg catgtgcccg cggacccaga actccttgtc ggtcacgtcc ttgtagccgg
      421 cgcaggagta gtcggcctcg ttgacataga ccaccgccgt gagctgctcc atctcgaacc
      481 gctgcgggtt gtagcggcgg atgtcgtcga gcgtcgcgac cgggtgctcg tagtcgaaga
      541 gcgacaggtc gatcagataa tctttctcgg ccacaagtcc caccctgagg gaacgaggca
      601 acaggcgacc gcggcgggct taggtgcgga ccttgcggcg cttggcccgg cgggccttgg
      661 cattggccgg gcgcttgccg tggttggcct tcttggcggt gcgatggggc ttggccatac
      721 gtgaatcctc tggcattaga cgtgaaaatc gggggcaatc gctcaagcta ccagcaatcg
      781 cgaatgttga gaaggcgatt tgcgctacag ctcgctcaac ttcggcagca cccagggctt
      841 acggtattcc gcgcgggtca ggtactggtt ggcgtcggcg tcgccagcga aggtgtaagt
      901 cgcggggtcg aaccgcagcg tgcggccggc ccgccaggcg gcgttgccga tgtggcagag
      961 caggctcgac ggatggccga tcgtctccag gtcggcgttg ggctttccgc ggctccgcat
     1021 gcaatcgagg aagttcttgg cgtgggccac gtcgttgttg tagtccccct tttcctcctt
     1081 gatctgcttg cccttggcgt cgaaggcccg ccagcgcgtg ttgccgatca cgacgaagcc
     1141 gttgtcgccg cagaccgcag cgccttcgga ctcttcgtgc agcggatacg aattccagac
     1201 ccgcatctcg tacgtcatca ggtagttggg cgcgaagtcg taggtgacca tcagcgtgtc
     1261 gggccattcc tggatgtcat cgaaatagta cttgccgccc aaggcgctga ccgcccgcgg
     1321 caagtcgggc agcgtctcct tctgggcggc cgcggcgctg gccagcgccc agcggcaata
     1381 gtccagccgg tgcacgccgt cgttgcccag gtcgccggtt cccaggtcga agaaccaccg
     1441 ccagttgccg tgaaaccgca tgcggttgaa gggcttcttc ggcgcggggc cgagccaggt
     1501 gtcgtagtcg aagcccgccg gcggatcact gtcggccggc ttgccgagcg agccctgctt
     1561 ggcgctctcc cacgccttgg cgaagcggac tttgccgagc gcgccggtct tcagatagtc
     1621 catacacgag aagaaatgct gcccgctgcg cgactgagtg ccgagctgca cgacgcggcc
     1681 gtacttcttc gccgcggcga ccatcgtgcg gccttccagg atgttgtgcc cgtcgggctt
     1741 ctcgacatag acgtccttgc cggcctggca ggcgtagatc gtcggcagcg cgtgccagtg
     1801 atcgggcgtg cccagcacca gcgcgtcgag cgtcttgtct tccagcgcct ggcgaaagtc
     1861 cttcaggccg atcggcttct tgccggtttt ctgctccacc tgcgccagcc gcgactcccg
     1921 cacctgctcg tcgaggtcgc agacgtaccg cacctccacc tcgggcaggc ccgcgaacgt
     1981 cgccagcacg ccggcgccgc gcccgcggac gcccatcatg cagatcgaaa tcttgtcgct
     2041 gggcgatcgg ttctgggccc ttgccgcgga ggtcagcgag gcgaaggcgg tcgcggctgc
     2101 ggacgcttcg agaaacgagc ggcggtccat ggcgggactc cggagtggag acgcgggagc
     2161 aaacgaacgc ggcccgctac acggagtgca gcgggccgca cggaaatgtc atctgcggtc
     2221 ggcgaactag gcgcccttgt gaaccgagtg gcacgccatg caattcgcgg cggcccgcag
     2281 cttcgcgccg gcgccttcct tgccttcagc ggcttccttc gccgcagcca acagggcggc
     2341 ggtcttctcc ttccaactgg cctcgtctcc cttcggcggc ttgttggccg ccagcgcggt
     2401 gtacagctcg accagctgct tcttctcgtc cgcgttggcc tcgcccttgg cgaccttcgc
     2461 catcagaccg cccttatgtg cggtcttcat cacgtccttg atcgtgacct ttttgtcttc
     2521 ctgtgccgtg ctcgtcaggc tgacgaccaa ccagcccaaa gcgaccacga cggctgccac
     2581 acacattttg cgcataccag gtctccgctc tcgtgaatga aacttggaag tgaaacccta
     2641 cgtgcggctg aaatgataca ccaccgccaa agcaatttcc tcagccccac gccccgcggc
     2701 gcggatttct tgggacaccc cacttgaggg gcttgcgtcg tgccggtaaa ggtcatcgac
     2761 catccgactg accgatcacc cgccccgtct ttggatcgat ttccaggccg agctgctcat
     2821 cgtcgtgcgt gtagaacaga aagccgtaca tcatctcctg aaacgtctgc tcgccttcct
     2881 tcacggtggc cttggggtcg gggttgtagg gattgaaggc cgagttgtcg aagtgggcgg
     2941 tgacttcgta cttcgtgccc ttctggaact ttttctgccc ggcggcccag cggtaggaaa
     3001 tctgccagtc gaaactgtag ttgggaatcg ccagcaggac ttcgctccgc tctggatcgt
     3061 cgccactcgc gggatacctc gcccggaacg tcatgtcctt gccccgcagg tgcatgtgcg
     3121 cgaacatgcc gatgccggtc gagtcggccg cgaaggtccg cgtcgcgctc accgggtgat
     3181 gcgacgcgcc gggcggaatc tcgaaccggc ggttcacgcc ctgaaagtgc cgcagctcct
     3241 tctggatcgg ctccttggcg aagcccagcc caacgctggt ccggtcggtc gccttcatgc
     3301 ccgtcgtcac gtagtggatt tgcagggcca gcaccgagcc ggcgggaatc ttgaacccca
     3361 cgccgccgtc cagcaccatc gcgtcaccgc cgggcacgta gcccgtgatg aagttctcgc
     3421 tagccggcgg cgcgccgacc ttcacgtacg ccaggttcgc gtggtgcacg gccgatttgt
     3481 tgcccggcag gatttccacc cgctgcaccc aggtgtcctg caggaagaca tgcggcagca
     3541 gcacgtagcg ataggggaca atgccctcgg ccggaatctc ctgcgtcgtc acgagcttca
     3601 gctccaggtc cggctcgccg atcttccagc ggccgctggc aaactcgcgc ggcggcggca
     3661 gctcgctcgg gtcgccttcc agcttgccgc tccgggccca ggccagcagc agggcccttt
     3721 cctggtcgct gaggcggcgg gcgttgacga tctgggtctg ctcgcggctg gcgaaagccg
     3781 gcggcatccg ctcctgccgc acgacttcgg ccagcgtctc ggcatggtcc cgcgcgtcct
     3841 tgaagctgag cagcgagaac ggcgcggagg tctccggccg gtggcactcc tggcagtgct
     3901 tggccaggat cggcgcgacg tgcctggtgt acgtcacctc cttcggcacg gcagccggca
     3961 gcgccgccag cgcatcgaat tcgatcaagc agccgtcaac aggcgtcgtc gcgacgcgca
     4021 cctcgcggcc ggcgaggacg tcctcgatcg cttccttcag gtcttcccgg gt
//
LOCUS       DASRJB010000259         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2329558, whole genome shotgun sequence.
ACCESSION   DASRJB010000259 DASRJB010000000
VERSION     DASRJB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329558"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1607
                     /locus_tag="VFB80_06990"
     CDS             63..1607
                     /locus_tag="VFB80_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN33545.1"
                     /translation="MLASWTKLLTISIAAALVGCKSGDPAPETPPWLAMQPLVPPVPV
                     DAAPPAVATASWNAPAEGSDSVFRPVTITAQPPAVLPPPPQPPAEAIVPALTLLDAVE
                     AGLAQNPELNALRYAEGVSEGAVGVAHAPLFNPYVQIQVTPGQHSQNPDTNVGPVSHY
                     VLLQQQLQIAHQRRHRTAAAAAALNSVRWNFVQARLVNIATTQRLYFAALYQRGLAEI
                     ARANADVNAQLLDVSQKQFAAGEAAGVDVAILRLDSAAASRQALLAAANYETALLDLR
                     RHLGVPPQASLALAGGLADWHWLPVDAEHAAWTPLVEMALPATSDKQLLAQALASGRP
                     DVWAAASDVEAAQENLSLARASRVPDLIIGPYYLRSNDPTTYWGLRAQMDIPILNSGM
                     PLVRQRLAELTQRHAVWEQLLSRATLDAYAALDRYERALLLAAEAPAAAGAQPPEELR
                     RLEAQFQAREVDLVRIVTARTSFFQARRAALDALNEVAQASAVVVAATGLPPEALLKP
                     APHLPQ"
     gene            complement(1592..2422)
                     /gene="accD"
                     /locus_tag="VFB80_06995"
     CDS             complement(1592..2422)
                     /gene="accD"
                     /locus_tag="VFB80_06995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZN33546.1"
                     /translation="MPTSKTAERKPKRGVPEGLWIRCPGCRASIYRKEAERLLNVCPQ
                     CEYHFYVSAQERIKQVLDDGTFEEWDAHLQPADPLSFADQKPYKERLVAEQQRTGLND
                     AAVTGGGMIRARRVAFAVTDSAFIMGSMGSVVGERLARLIERATEENLPLIIVSGSGG
                     GARMHEGILSLMQMAKVSAALARYDRAGGLFISVLTNPTMGGVAASFASLGDLIFAEP
                     KALIGFAGPRTIKATIRLELPKGFQTSEFLLSHGFVDRIVARSRLKSEIARAIDYCGK
                     "
     gene            complement(2426..3004)
                     /locus_tag="VFB80_07000"
     CDS             complement(2426..3004)
                     /locus_tag="VFB80_07000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN33547.1"
                     /translation="MVQILLVRACSTDFDEQGRIKGTLDIPLNEIGQGQATQLVADLH
                     NTDIDILYTSPCRSAIQTAEAIAADHKLRSKSLDDLQNLDHGLWHGKLIDEVRVSQPK
                     VFRQLQDRPETVCPPQGEAVGAALERVRTLVARLIRKHRKGTIALVVPEPLASLVSSA
                     LGAELGDLWKAECKCGGWEMIEVLPEKVALAS"
     gene            complement(3366..4121)
                     /locus_tag="VFB80_07005"
     CDS             complement(3366..4121)
                     /locus_tag="VFB80_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZN33548.1"
                     /translation="MDEQPWTAALGLDGLAEPDDQGLSLAELSQAYAAILNKGADPYP
                     EAAAPGSEAALAEPLASVEPPAALPAGDDADCEITPRTILEAMLFVGHPTGEPLLSER
                     IAALMRGVRPAEVDDLVRELNDEYQRSGAPYLIASVGAGYQLALRPEFSGLRDAFYGR
                     IREARLSQSAIDVLAIVAYHQPMTQAQIDQLRGKPSGGILSQLVRRDLLVLQRPVEAK
                     GKPTYRTTGRFLDLFGLDDLSELPRSQEIERNL"
     gene            4186..4662
                     /locus_tag="VFB80_07010"
     CDS             4186..4662
                     /locus_tag="VFB80_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33549.1"
                     /translation="MTQRSESAQPGQAATSRVPMLLVGLLLLANVVVPLVWGDVYPFT
                     SAPMFRDCPSCCCNYQVFAADGSELPAEDWLVQRVYDGNPVGYGVGVRPPAVIEQEFG
                     VVCGEAAVRQHFQEQLARPEHSQHAYVDVVQHVIGPLEYSPRVGVVQTNRWRVERP"
     gene            complement(4663..4947)
                     /locus_tag="VFB80_07015"
     CDS             complement(4663..4947)
                     /locus_tag="VFB80_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZN33550.1"
                     /translation="MFGLSFQELFLVAIVAILLFGKNLPDVAKKFGGFYRDFRKGLDD
                     LRSQVDFTDTFNAAPSRPRPRRVEYDDTDEITAPKFEPPPAPPEQTSDAA"
     gene            complement(5040..5213)
                     /locus_tag="VFB80_07020"
     CDS             complement(5040..5213)
                     /locus_tag="VFB80_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZN33551.1"
                     /translation="MFGIGHWELLLIVLVIFILFGHRLPGVMRSLGRGVVEFKEGLAG
                     TPKDEAGDEKKDA"
     gene            complement(5323..5502)
                     /locus_tag="VFB80_07025"
     CDS             complement(5323..5502)
                     /locus_tag="VFB80_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZN33552.1"
                     /translation="MIGGIGYWELLLIAFVVFLLFGHRLPSVMGSVGEGIRSFKRAMS
                     GQDEEPAASDSSIHR"
BASE COUNT          997 a         1932 c         1897 g          986 t
ORIGIN      
        1 cgaaggaatc ttcgtcagac tctgcgcgca cggcagggtc gccgtcgcga tagctgctag
       61 cgatgcttgc atcttggacc aaactcctga cgatctcgat cgcagccgcg ctcgtcggct
      121 gcaagagcgg cgatcccgcg ccagagacac cgccctggct ggcgatgcag cctctcgtcc
      181 cgcccgtgcc ggtcgatgct gctccacctg cggtggcaac tgcgagctgg aatgcacctg
      241 cagagggctc ggattcggtt tttcgcccgg tgaccattac ggcgcagcct cctgcagtac
      301 ttccgccgcc cccgcagccg ccggccgaag ccatcgtgcc ggcgttgacg ctgctggacg
      361 cggtggaagc gggcctcgcg cagaacccgg agctgaatgc cctgcgctat gccgagggcg
      421 tcagcgaggg cgcggtgggc gtcgcgcatg cgccgctctt caatccgtac gtgcagattc
      481 aggtcacgcc cggccagcac agccagaatc cagatacgaa cgtcggaccc gtcagccact
      541 atgtcctctt gcaacagcag ctgcagatcg cccaccagcg gcggcaccgc acggctgccg
      601 cggcggcagc gctcaacagc gtccgctgga acttcgtgca ggccaggctg gtcaatatcg
      661 ctaccaccca gcggctctac ttcgccgccc tttatcagcg cgggctggcg gagatcgccc
      721 gggccaatgc cgacgtcaac gcgcagcttc tggacgtctc gcagaagcaa ttcgccgccg
      781 gcgaagctgc cggtgtcgat gtcgcgatcc tccgactcga cagcgccgcg gctagccgtc
      841 aggcgctgct ggccgccgcc aactatgaaa ccgccttgct cgacctgcgt cggcacctgg
      901 gtgtgccgcc acaagcgtcg ctggctctcg ccggcgggct ggccgactgg cactggctgc
      961 ccgtcgatgc cgagcatgcc gcgtggacgc cgctggtcga gatggcgctg ccggccactt
     1021 cggacaagca actgctcgcg caggctctgg cctcaggccg gccagatgtc tgggcggcgg
     1081 cctccgacgt agaagccgcg caggagaacc tgagcctggc ccgcgccagc cgcgttccag
     1141 acttgatcat cgggccgtac tacctgcgct cgaacgatcc cacgacctac tggggcctgc
     1201 gggcccagat ggatattcca atcctcaaca gcgggatgcc gctggtccgc cagcgcctcg
     1261 ccgagctaac acagcggcat gccgtttggg agcaactcct cagccgcgcg acgctcgacg
     1321 cttatgcggc cctggatcgt tacgagcggg cgctgctgct ggccgccgaa gcgcccgccg
     1381 cggccggcgc gcaaccgccg gaggagctga ggcgcctcga ggctcagttc caggcccgcg
     1441 aagtcgatct cgttcgcatc gtcacggctc gaactagttt ttttcaggcc cgccgcgccg
     1501 cactcgacgc gctgaacgaa gtggcgcagg cgtcggccgt tgttgttgcg gcaaccggcc
     1561 tgccgccgga agcgctgctt aagccggcgc ctcacttgcc gcaatagtcg atcgcccggg
     1621 cgatctcgct cttcagccgc gaacgcgcaa cgatgcgatc gacgaagccg tgcgacagca
     1681 ggaactcgct ggtctgaaag cccttgggca gttccaggcg gatcgtggcc ttgatggtcc
     1741 gcgggccggc aaagccgatc agcgccttgg gttcggcgaa gatcaggtcg cccagcgagg
     1801 cgaagctggc ggcaacgcca cccatcgtcg gattggtcag gaccgagatg aacaagccgc
     1861 cggcccggtc gtagcgggcc agcgcggccg acactttggc catttgcatc agcgacagga
     1921 tcccttcgtg catcctcgcc ccgccgccgg acccgctgac gatgatcagc ggcaggtttt
     1981 cctcggtcgc ccgctcgatc agccgggcca gccgctcgcc gaccaccgag cccatgctgc
     2041 ccatgatgaa cgcggaatca gtgaccgcga atgccacgcg gcgggcgcgg atcatgccgc
     2101 cgccggtgac ggcagcgtcg ttcaggcccg tccgctgctg ttcggcgacc agccgctcct
     2161 tatagggctt ctggtcggca aagctcagcg ggtcggccgg ctgcaagtgg gcgtcccatt
     2221 cttcgaaggt gccgtcgtcc aggacctgct taatccgctc ctgggcggaa acgtagaagt
     2281 gatactcgca ctgcggacaa acgttcagca gccgctcggc ctctttgcgg taaatcgacg
     2341 cccggcagcc ggggcagcga atccagagtc cctcgggcac gccccgcttg ggttttcgct
     2401 cggccgtttt cgaggtaggc atgccttagc tcgccagggc taccttctcc ggcagaactt
     2461 cgatcatttc ccagccgccg cacttgcact cggccttcca gaggtcgccc aactcggctc
     2521 ccagggcgga gctgaccagg ctggccagcg gctcggggac gaccagggcg attgtgccct
     2581 tgcgatgttt gcggatcaag cgggcaacca gcgtccggac gcgttccagg gcggccccga
     2641 ccgcttcgcc ctgcggcggg cagaccgtct cggggcggtc ctgaagctgg cggaagacct
     2701 tgggctggct gacgcggacc tcgtcgatca gcttgccatg ccacaggccg tgatcgaggt
     2761 tttgcaagtc gtcgagcgat ttggaccgca gtttgtgatc ggcggcgatg gcctcggcag
     2821 tctggatcgc cgagcggcag ggggacgtgt acaggatatc gatgtcggta ttgtgcagat
     2881 cagccaccag ctgggtggcc tgtccctggc cgatttcgtt cagcggaatg tccagcgtac
     2941 ccttgatccg gccctgctcg tcgaaatcgg tcgaacaggc ccgcaccagc aagatttgaa
     3001 ccatagattt cgcggtcacc tcatgtcaaa actgcgccta gctgaatctg tcgcaaatcg
     3061 cggaccagca cgggcagagg aggtcggcgt aataccgacc gggcgagctc gacgagcccg
     3121 gcccaagcgt ttcagtctgg aagaagcacg gcggccagtt ggctaccact gctgaatgtc
     3181 ctgtggggga ggcaagggac cgagcgtccc atgcttcatt tccaacaggg ttccattcta
     3241 cagggcgtca accaccgcgt cagtatctct tcggcaaccc gcactcctgt cgccacccgg
     3301 atgatgggat agcgggtccg cttaacgcaa gtcattgcgc aggaacaact tgcggatttc
     3361 ccatctcaca agttccgctc gatctcctga ctccggggga gttcggagag gtcgtccagg
     3421 ccgaacaaat cgagaaaccg gccggtcgtc cgataggtcg gcttgccctt ggcttcaacc
     3481 gggcgctgca gcaccagcag gtcgcgccga accagttgac tcagtatccc gccgctgggc
     3541 ttgccgcgca gctggtcgat ctgggcctgc gtcatgggtt ggtgataggc gacgatggcc
     3601 agcacatcga tggcactttg cgagagtcgg gcctcgcgaa tccggccgta aaaggcgtcc
     3661 cgcagcccgc tgaattcggg ccggagggcc agctgatagc cggccccgac cgaggcgatc
     3721 aggtagggcg cacccgaccg ctggtattcg tcgttcagct cccgcacgag gtcgtcgact
     3781 tccgccggcc ggactccacg catcagggcc gcgatccgct cgctcaagag cggctctccc
     3841 gtcgggtgac cgacaaacag catcgcttcc aggatggtcc gcggcgtgat ctcgcaatca
     3901 gcgtcgtcgc cggcaggcag ggccgcgggc ggctcaacgg atgctagcgg ctcagccagc
     3961 gccgcctcgg aaccgggggc ggccgcttcg ggataggggt cggccccctt gttcaaaatg
     4021 gcagcgtagg cctggcttag ttcggcgagc gacaggccct ggtcgtcggg ctccgccagc
     4081 ccgtccagtc ccaatgccgc cgtccagggc tgctcgtcca ttctgcgact ataggggcct
     4141 ttcgcggcgg gcggcaagcc gaggttccgg tatagtggga acgccatgac gcaacgatcg
     4201 gaatcagctc agcctggcca agccgcgacg tcgcgggtgc caatgctgct ggtcggcctg
     4261 ctgctgttgg ccaacgttgt ggtgccgctg gtgtggggcg acgtgtaccc gtttacgtcg
     4321 gccccgatgt ttcgcgactg cccgagctgc tgctgcaact atcaggtctt tgcggccgat
     4381 ggttcagaac tgccggccga ggattggctg gtgcagcggg tttatgacgg caacccggtc
     4441 ggctacggcg tcggagtacg cccgcccgcc gtcatcgagc aagagttcgg cgtcgtgtgc
     4501 ggcgaggccg ccgtccgcca acattttcag gagcagctgg ctcggcccga gcacagccaa
     4561 cacgcgtacg tcgatgtcgt ccaacacgtc atcggcccgc tggagtacag cccgcgggtc
     4621 ggcgtcgtcc agaccaatcg ctggcgggtt gagcggccgt aatcaggcgg cgtcgctcgt
     4681 ctgttccggc ggggccggtg gcggctcgaa cttgggggcg gtgatttcgt cggtgtcgtc
     4741 gtattccacg cggcgcgggc gcggccggct gggagcggcg ttgaaggtgt cggtgaaatc
     4801 cacctgcgac cgcaagtcgt cgagcccctt gcggaagtcg cggtaaaagc cgccgaactt
     4861 cttggccacg tccggcaggt tcttgccgaa cagcaagatc gccacgatcg cgactaggaa
     4921 cagttcctga aagctgagtc caaacatggc tgggcaatcc gggcgagagg gaacgatcgc
     4981 ccgcgccggg ccgcggcaag tgaccgcagg ccaacgaagc agcgcgggca gaagaacggc
     5041 taggcgtcct tcttttcgtc gcccgcctca tccttgggcg taccggccag cccttccttg
     5101 aactcgacca cgccgcggcc cagcgagcgc atcacgcccg gcagccgatg gccgaagaga
     5161 atgaagatca ccagcacgat cagcagcagt tcccagtgtc caatgccaaa catggtcgct
     5221 ctcgctcaat aaggaaggcg ggtgggcaaa cgcgagcggc gaaagcgggc gccgcccagt
     5281 tgggcagcgc cagaacctgc tcgaaaccaa acgcggattg ttctaacggt gaatcgagga
     5341 atcgctcgct gccggctctt catcctggcc ggacatcgcg cgcttgaagc tccgaatccc
     5401 ctcgcccacc gagcccatca cgctgggcag tcggtgaccg aagagcagga acaccacaaa
     5461 cgcgatgagg agcagttccc agtagccgat gccaccaatc atggcagtct cccgtttcgc
     5521 aaaaggacag cacgccaaaa caggcgagcg gcgaaagcgg gcaaccgccc caacgggcag
     5581 ggccagaacc gactcgtcaa caacttgttt atattaccac ccgcgggcgg gcggtgtcaa
     5641 atagtaggcc gctcgctccg cgagcggcag cctcacgcgg agcgtgagga ctactgttat
     5701 gatcgcgggc caatctggat ccgcggggag ggcgtgggca ggttcagccg ggcgtcgagg
     5761 accttggcga tccgcgcgcc ggtctcctgc aggtgggccc ggctgtaggt gt
//
LOCUS       DASRJB010000260         4936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_357182, whole genome shotgun sequence.
ACCESSION   DASRJB010000260 DASRJB010000000
VERSION     DASRJB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357182"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VFB80_07030"
     CDS             complement(<1..1914)
                     /locus_tag="VFB80_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN33553.1"
                     /translation="MRDAIELLPQVTISRRTLLGWAAGAAGMTVLPPIGAELELRAQQ
                     PAAGAARTEPVPLNRFSRMVQEWFVDQVRAAEDRIKQRLASVKTKADAEAYVRSVQER
                     IRECFGPEPERTPLNARVAGAIERDAYRIEKIIFESRPNFPVTANLYIPTGRKFPLPG
                     VVGSCGHSATGKAIEAYQSFAQGLARLGYVMLIFDPIGQGERLQYVDEDLKPRRGIGV
                     SEHLHAGNQQTLVGESFAAWRAWDGIRTLDYLLTRKEVDPAHVGVTGNSGGGTMSTWL
                     LGLDRRWTMGAPACFVTTFRRNLENELPQDTEQCPPRAIALGLDHADFLAAMAPKPVI
                     ILTKEKDYFDVRGSIETFARLKHLYTLLGKPENIALHTGPTEHGYSQENREAMYRWFN
                     RVTGISDAQSEPALSIEKDEVLWCTPRGQVAESKPRTVFSFTEEKSKSLAAKRGEIRG
                     EALKAAITDVLKLPTLPAEPPDYRILRPLRGARNYPRRFATTYAVETEPGIHALVTRL
                     SAVSHLSRPPQDPRPATLYVSHHSADMELRSELLIKELMTDEDAPFFACDLRGIGESR
                     PDTCGSDQFQSPYGSDYFYSAYSLMLGRPYVGQETLDLLRVLDWLASLGHREVHPAAK
                     GWGAIPAAYAAVLR"
     gene            complement(1935..2180)
                     /locus_tag="VFB80_07035"
     CDS             complement(1935..2180)
                     /locus_tag="VFB80_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZN33554.1"
                     /translation="MNVSVKLFAVARQRAGRGNIIVELPAGATVQQLRAALAAQCPPL
                     ADLLKHSRVAVNSDYAGDAAAIPESAEIALIPPVSGG"
     gene            complement(2234..>4936)
                     /locus_tag="VFB80_07040"
     CDS             complement(2234..>4936)
                     /locus_tag="VFB80_07040"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HZN33555.1"
                     /translation="SMRRIVSVNGDTVTVQPGVVLGQLNRYLAAFGRQFGPDPATGGV
                     TTMGSVLALDNSGSNWLRYGSARRHVVSMQVVFADGETMEVGRHPVTDDPHKDPLPRR
                     RDLVRRLAELIERERPLIEAHRPKAWVNRCGYHLYDVLQDGHLDLARLLVGSEGTLAL
                     IAQATLRTVPIPKCRGVALLFFDRLEAAAVAATEAQSLGLAACDLIDRRILSLARDLD
                     DRYARAIPREAEAMLIVEQQADEERDVIDGLQQAVIRWQRRQRLAFGFHMALAPDEFD
                     AFRRLARRVSPTLYRVQGAERPTPFVEDIAVPPAALPDFLVRLQNVLKAHDATASFFA
                     HAGHGQLHVRPFLKLADAGDVSKMQAIAEELYEEVLKVGGTISGEHGSGLSRTWFLRK
                     QFGPLYDVFREVKRIFDPHSLLNPGKVIADVPQPLTKNLRLSEPLALMTETTAATEAA
                     EPEEDIKPLELQLIWDGRLAAEANLCNGCGRCRTQSPEARMCPIFRIAPAEEASPRAK
                     ANVMRGIISGQLDPALLEKDELKEIADLCVNCHQCRLECPAAVDIPKLMIEAKAQFVA
                     ANGLGTADWVLTRLDRIAAWSSRLATVANYALHTRWTRWLLEKGVGIAQGRKLPRVSP
                     QSFLRWAERRRLTRPARRSGGKVVYFVDLYANWYDVSLAEALVAVMEHNNIAVYVHPD
                     QEPSGMNRIAVGDVERARRTAARNVRLLADAVRQGYEIVCTEPSAALCLTHEYPDLLD
                     DDDVKLVATHTHEACTYLWRLHEAGKLELNFQPLNLNFGYHLPCHLRALEVGSPGEQL
                     LKLIKGVTVRRIERGCSGMAGTFGLKRENYRASLRAGWGLIRAMRDPGVQLGTTECSA
                     CKIQMEQGTTKPTIHPLKVLAIAYGLMPELQELLSARSGELTVT"
BASE COUNT          773 a         1618 c         1710 g          835 t
ORIGIN      
        1 ccggaggacg gcggcgtaag ccgcggggat cgcgccccag cccttggcgg ccgggtgcac
       61 ctcgcggtgg cccagcgagg cgagccagtc gagcacgcgg agcagatcca gggtctcctg
      121 gccgacgtac ggccggccca gcatcagcga gtatgccgaa tagaaatagt cgctgccgta
      181 ggggctctgg aactggtcgc tgccgcaggt gtcgggccgc gattcgccga tgccgcgcag
      241 gtcgcaggca aagaacggcg cgtcctcgtc ggtcattagc tccttgatca gcagctcgct
      301 gcggagctcc atgtcggccg aatggtgcga cacatagagc gtcgctggcc gcgggtcctg
      361 cggcgggcgc gaaaggtgcg agactgcgct caagcgcgtc accagcgcgt gaatgcccgg
      421 ctcggtctcg acggcgtacg tcgtggcaaa gcggcgggga taatttcggg ccccccgcag
      481 cggacggaga atgcgatagt cgggcggctc ggctggcagc gtgggcaact tgaggacgtc
      541 ggtgatggca gctttcaagg cctcgccgcg gatttcgccc cgcttagcag ccagcgactt
      601 cgacttctcc tcggtgaacg agaacaccgt ccgcggtttg gattcagcga cctgcccgcg
      661 cggcgtacac cacaggactt cgtccttttc gatcgaaagc gccggctcgc tctgggcgtc
      721 ggaaatgccc gtgacgcggt tgaaccagcg gtacatcgcc tcgcggtttt cctgcgaata
      781 gccgtgctcg gtcgggccgg tgtgcagggc gatgttctct ggcttgccga gcagtgtgta
      841 gagatgcttg aggcgggcaa acgtctcgat ggagccgcgg acatcgaaat agtccttctc
      901 cttggtcagg atgatgaccg gcttgggggc catcgcggcg aggaaatcgg cgtggtccag
      961 gcccagcgcg atcgcccgcg gcgggcactg ctcggtgtcc tgtggcagtt cgttctcgag
     1021 attgcggcga aacgtggtca cgaagcaggc gggcgcgccc atcgtccagc ggcggtcgag
     1081 gccgagcagc cacgtgctca tcgtgccgcc gccggaattg ccggtgacgc cgacgtgggc
     1141 gggatcgacc tccttccgcg tcagcagata gtcgagggta cggatgccgt cccaggcccg
     1201 ccaggcggca aaactctcgc cgacgagcgt ctgttggttg cccgcatgga ggtgctcgct
     1261 gacgccgatc ccgcgccgcg gcttcaggtc ttcgtccacg tactgcagcc gctcaccttg
     1321 gccgatcggg tcgaagatca gcatcacgta gcccaggcgg gccagcccct gggcaaagct
     1381 ctgataggct tcgatcgcct tgcccgtcgc cgaatgtccg cagcttccca ccacgcccgg
     1441 cagcggaaac ttgcggccgg tgggaatgta aaggttggcg gtcaccggga aattcggccg
     1501 gctctcgaaa atgatcttct cgatgcgata ggcgtcgcgc tcaatggcgc cggcgacgcg
     1561 ggcgttcagc ggcgtccgct ccggctccgg accaaagcac tcgcgaattc gctcctggac
     1621 ggagcggaca taggcttcgg cgtcggcctt cgtcttcacg gacgccagcc gctgcttgat
     1681 ccggtcttcc gcggcccgca cctggtcgac gaaccattcc tgcaccatcc gggagaagcg
     1741 attgagcggc accggctccg tccgcgccgc accggccgcc ggctgctgcg cgcgaagctc
     1801 aagttcagcg ccgatcggcg gaagaacggt catgccggcc gccccggcag cccagcccag
     1861 cagcgtgcgg cgagagatgg tcacttgcgg cagcagctcg atggcgtctc gcatggcttg
     1921 gctcccagtc agattcagcc gccgctgacg ggcggaatca gggcgatctc ggccgattcg
     1981 gggatggcgg ccgcatcgcc agcgtaatca ctgttaacgg ccacgcggct gtgtttcaag
     2041 agatcagcca gcggcgggca ctgtgcagcc agcgccgcgc gaagctgctg caccgtcgcg
     2101 cccgctggta actccacgat gatgttgccg cggcccgccc gctggcgggc gacggcgaag
     2161 agtttgacgg agacgttcat cgtttgattg cagtcctcag ggggctcacg ccccccgctc
     2221 gccttcggct cgcctatgtg acagtgagtt ctccgctccg cgccgacagt agctcttgca
     2281 gctcgggcat caacccatag gcgatcgcga ggaccttcag cgggtgaatc gtcggcttgg
     2341 tcgtgccttg ctccatctgg atcttgcacg cgctgcactc ggtagtgccc agttgcacgc
     2401 ccggatcgcg catcgcccgg atcaggcccc agccggcccg caggctggcg cggtagttct
     2461 cgcgcttcag gccaaacgtg cccgccatgc cgctgcaccc gcgctcgatc cgccgcaccg
     2521 tcacgccctt gatcagcttt agcagctgct cgcccggcga gccgacctcc agggcccgca
     2581 ggtggcaggg cagatgatag ccgaaattca ggttcagcgg ctgaaagttc agctccagct
     2641 tgccggcctc gtgcagccgc cagaggtagg tgcaggcctc gtgcgtatgg gtggcgacga
     2701 gcttcacgtc gtcgtcgtcg agcaggtcgg gatattcgtg cgtcaggcag agggcggcgc
     2761 tcggctcggt gcagacgatc tcatagccct gccgcacggc gtcggccagc aggcggacgt
     2821 tgcgggcggc cgtacggcgg gcccgctcga cgtcgcccac ggcgatgcgg ttcatccccg
     2881 agggctcctg gtcgggatgg acatacacgg cgatgttatt gtgctccatc acggccacga
     2941 gggcctcggc cagcgagacg tcgtaccagt tggcgtacag atcgacgaaa tagacgacct
     3001 tgccgccgct gcggcgggcc ggccgtgtca accgccggcg ctccgcccag cggaggaagc
     3061 tctgcggtga gacccgcggc agcttgcggc cctgggcgat gccgacgcct ttctccagca
     3121 gccaccgcgt ccagcgcgtg tgcagggcgt agttcgcgac ggtcgccagc cgcgacgacc
     3181 aggcggcgat gcgatccagc cgcgtcagca cccagtcggc cgtccccagg ccattggcgg
     3241 cgacgaactg ggctttggct tcgatcatca gcttgggaat atcgacggcc gcggggcatt
     3301 ccaggcggca ctggtggcaa ttgacgcaca ggtcggcgat ttccttcagc tcgtcctttt
     3361 ccaggagcgc cggatcgagc tggcccgaaa taatgccccg catgacgtta gccttggctc
     3421 gcggcgaagc ctcttccgca ggagcgatcc ggaagatcgg gcacatgcgg gcttcgggcg
     3481 actgcgtccg gcagcggccg cagccgttgc agagatttgc ctcggcggcc agccggccgt
     3541 cccagatgag ctgcagctcg agtggcttga tgtcttcttc cggttcggcg gcttcagtcg
     3601 ctgctgtagt ctcggtcatg agggccagcg gctccgacag ccgcaggttc tttgtcagcg
     3661 gctgcggcac gtcggcgatc accttgccgg ggttgagcag gctgtgcgga tcgaaaatcc
     3721 gcttcacttc gcgaaagacg tcgtacagcg ggccgaattg tttgcgcagg aaccaggtgc
     3781 ggctgagtcc gctgccgtgc tcgccactga ttgtgccgcc gaccttgagg acttcctcgt
     3841 acagctcttc ggcgatcgcc tgcattttgg aaacgtcgcc ggcgtcggcc agcttcagga
     3901 acggccggac gtgcaattgt ccgtggcccg cgtgggcaaa gaacgaggcc gtcgcgtcgt
     3961 gggccttgag gacgttttgc agccgcacga ggaagtcggg cagcgcggcg ggaggcacgg
     4021 cgatgtcctc gacgaacggc gtcggccgct cggcgccttg cacgcgatag agcgtcggcg
     4081 aaacgcggcg ggccaggcgg cggaaggcgt cgaactcatc gggggccagg gccatgtgaa
     4141 agccgaaggc cagccgctgc cggcgctgcc agcggatgac agcctgctgc agtccgtcga
     4201 ttacgtcccg ctcttcgtcg gcctgttgct cgactatcag catcgcttcg gcttcgcgcg
     4261 ggatcgcgcg ggcgtagcgg tcgtcgaggt cgcgggccag cgagaggatg cggcgatcga
     4321 taaggtcgca cgccgcaagg cccagcgact gagcctcggt tgcagcgacg gccgccgctt
     4381 ccagccgatc gaagaagagc agggcgacgc cgcggcactt gggaatcggc acggtgcgga
     4441 gcgtcgcctg ggcaatcaag gccagcgtcc cttcgctgcc gacgagcagg cgggccaggt
     4501 ccaagtggcc gtcctgcagc acgtcataga ggtgatagcc gcagcgattg acccaggcct
     4561 tggggcggtg cgcctcgatc agcggtcgct cccgctcgat caattcggcg aggcggcgga
     4621 ccaggtcgcg ccgccgcggc agcgggtcct tgtgcggatc gtcggtgacg ggatggcggc
     4681 cgacttccat ggtctcgccg tcggcgaaga cgacctgcat gctcaccacg tgccgccggg
     4741 cggagccgta ccgcagccag ttgctgccgg aattgtcgag ggccagcacg ctgcccatcg
     4801 tggtcacgcc gccggtcgcc ggatcggggc cgaactgccg gccgaacgcc gcgaggtagc
     4861 gattgagctg ccccagcacg acgccgggct gcaccgtgac cgtgtcgcca ttgacgctga
     4921 cgatccgccg catcga
//
LOCUS       DASRJB010000261         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2612670, whole genome shotgun sequence.
ACCESSION   DASRJB010000261 DASRJB010000000
VERSION     DASRJB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612670"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFB80_07045"
     CDS             <1..277
                     /locus_tag="VFB80_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HZN33556.1"
                     /translation="VIGRQEELACRDLVAARCNWLAEEPAGEFRCTAKIRYNSPPAAA
                     TAETLPAGRLRVAFDEPRHGVAPGQAVVLYEGERVLGGGWIESELAN"
     gene            471..1436
                     /gene="floA"
                     /locus_tag="VFB80_07050"
     CDS             471..1436
                     /gene="floA"
                     /locus_tag="VFB80_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095100.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HZN33557.1"
                     /translation="MGNFGIILVLGIAGIAAIVLLIAAIAFAAYGKLWFQAYMSSADV
                     SMLALIGMGFRQVNSRIIVQAKIMAAQAGLDINRTSGISTKRLEAHYLANGNVTNVIL
                     AIISAHRAGIDLDFDRAAAIDLAGRDVLDAVRTSVMPKIIDCPDSRSGKPTLSAVARD
                     GIELLVKARVTVRTNLAQLIGGATEETIIARVGQGIVTSIGSSKTHKDVLANPNNISR
                     ILLDRGLDAQTAFEIVSIDIAKIDIGQNIGARLQADAAEADTRVARALAEQRRAEAVA
                     AEQEMKAKVAENRSRLVEAESQVPMAMAEAFRKGNLNGASVAAAR"
     gene            1539..>2299
                     /locus_tag="VFB80_07055"
     CDS             1539..>2299
                     /locus_tag="VFB80_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN33558.1"
                     /translation="MPTLILLALLSADPSPEQLKLLKMFKEEFVAIKIEGSNLKPYSI
                     GKYEVPQDLWAAVMSDNPSRWLGPRNSVDRMRLSEAKRFCVRSTDLMRRAGLIDQGQL
                     IRLPTEAEWEYAARAGTKTKYSFGDDPAQLGDYAWFTGNAKGNDPPVGAKKPNPWGLY
                     DMHGYQWEWTESGVLRGGSWKDSAKFLTSDSRLEAPAGASDDAFGLRCVLALVKDAPE
                     SRPAEFRPTAQEKFVPASSKLEMLWNEGEFTEGPAL"
BASE COUNT          436 a          770 c          732 g          361 t
ORIGIN      
        1 ggtcatcggc cggcaggagg agctggcctg ccgcgatctg gtagccgccc gctgcaattg
       61 gctggccgaa gagcccgccg gcgaattccg ctgcacagcc aagatccgct acaacagccc
      121 gcccgcagcc gcgaccgcgg aaacgctgcc cgccggccgg ctgcgcgtcg ccttcgacga
      181 gccccgccac ggcgtcgccc ccggccaggc ggtcgtgctg tacgagggcg agcgggtgct
      241 gggcgggggg tggatcgagt cggagcttgc aaattgagaa ttgcaaaatg caaagtgcaa
      301 attgagggac gaattccggc tgcgtcccaa tttgcacttt gcaatttcca gttgcagttt
      361 gcaatcaccc gctcgtcctc gctcctcgcc tccttccgct attccccgcc gccggaatcg
      421 tctatcatct cgtcaggttc catcgaagag taacacacgg ggggcggcac atgggcaatt
      481 ttggcatcat cctggtgctg ggtatcgccg gcatcgcggc gatcgtcctg ctgatcgcgg
      541 ccatcgcctt cgccgcttat gggaagctct ggttccaggc ctacatgtcc agcgccgacg
      601 tgagcatgct cgccctgatc ggcatgggct tccgccaggt gaattcgcgg atcatcgtgc
      661 aggccaagat catggcggcc caggcgggcc tggacatcaa ccgcacgagc ggcatcagca
      721 cgaagcggct ggaagcgcac tacctggcca acggcaacgt gaccaacgtc atcctggcga
      781 tcatctccgc ccaccgggcg ggcatcgacc tcgacttcga ccgggccgcg gcgatcgacc
      841 tggccggccg cgacgtgctc gacgccgtgc ggacgagcgt catgcccaag atcatcgact
      901 gccccgattc gcgcagcggc aagccgacct tgagcgcggt ggcccgcgac ggcatcgagc
      961 tgctggtcaa ggcccgcgtg accgtccgca cgaatctggc acagctcatc ggcggcgcga
     1021 ccgaggaaac catcatcgcc cgtgtcggcc agggcatcgt cacctcgatc ggctcctcga
     1081 agacccacaa ggacgtgctc gccaacccga acaacatctc gcgaatcctg ctcgaccgcg
     1141 gcctcgacgc ccagaccgcg ttcgagatcg tctcgatcga catcgccaag atcgacatcg
     1201 gccagaacat cggcgcacga ttgcaggccg acgccgccga ggccgacacc cgcgtggccc
     1261 gcgccctggc cgagcagcgg cgggccgaag ccgtcgccgc ggagcaggag atgaaggcca
     1321 aagtcgccga gaaccgctcg cggctggtgg aggccgaatc gcaagtcccc atggcgatgg
     1381 ccgaagcctt ccgcaagggc aacctgaacg gggcgtcggt ggccgctgcg cggtgagtcg
     1441 gttgatcggt cgatcggttg gacggtcagc cggttgactc aataaccgac ctactgactt
     1501 accgaccgac cgacttaccg acacaccggt ccgctgctat gcccacgctc attctgctcg
     1561 cactgctctc cgccgacccg tcgcccgagc agctcaagct gctcaagatg ttcaaggagg
     1621 aattcgtcgc gatcaaaatc gaagggagca acctcaagcc ctactcgatc ggcaagtacg
     1681 aagtgccgca ggatctctgg gcagcggtga tgagcgacaa ccccagccgc tggctgggcc
     1741 cgcggaattc cgtggacagg atgaggctgt ccgaggcaaa aaggttctgc gtccggtcaa
     1801 ccgacctgat gcggagggcc ggtttgatcg accagggcca attgatccgg ttgccgaccg
     1861 aagccgaatg ggagtatgcc gcccgcgccg gcacgaagac gaagtactcc ttcggcgacg
     1921 atccggcgca gctcggcgat tacgcctggt tcaccggcaa cgccaaggga aatgacccgc
     1981 ccgtgggcgc gaagaagccc aatccctggg gcctgtacga catgcacggc taccaatggg
     2041 aatggaccga atccggcgtc ctgcgcggcg ggagctggaa ggactcggcg aaattcttga
     2101 ccagcgattc gcggctcgaa gcgcctgccg gggcgagcga tgacgcgttc ggcctgcgct
     2161 gcgtgctcgc cttggttaag gatgcgccgg agagcaggcc ggccgagttt cggcccaccg
     2221 cccaagaaaa attcgtcccg gccagctcca agctggaaat gctctggaac gagggcgagt
     2281 tcaccgaagg gccggccct
//
LOCUS       DASRJB010000262         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1767562, whole genome shotgun sequence.
ACCESSION   DASRJB010000262 DASRJB010000000
VERSION     DASRJB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767562"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..923)
                     /locus_tag="VFB80_07060"
     CDS             complement(414..923)
                     /locus_tag="VFB80_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN33559.1"
                     /translation="MIDYVRQVLAAQFEAGLCMLHQCVQKCQPQLWEGKIAGITFRQV
                     AYHTLFFVDLYLSPSEQAFVLRDLHATGGDEREPRFTPGLPQDETLAYVSLCRQKAAQ
                     ALAAETQESLAGPSGFSWYPLSRGELHLISLRHIQHHTGQLSAYLRRCDPLLADRSAL
                     RWVGSGWRS"
     gene            1049..1513
                     /locus_tag="VFB80_07065"
     CDS             1049..1513
                     /locus_tag="VFB80_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33560.1"
                     /translation="MLDRGKLAVLSMFALAVVAATFAWWWNYRRGERCLAFYGSEAAT
                     LIRTAQTVEIVELDPAAGDLAISRRIDISRAPGLLNARAALLDDASFDWSAPPPEPAS
                     GTRLVRFAEGDREVLLRLDSARRSLEIVPAGKSAVLDQKTSDGWRQFLERYP"
     gene            complement(1595..2020)
                     /locus_tag="VFB80_07070"
     CDS             complement(1595..2020)
                     /locus_tag="VFB80_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZN33561.1"
                     /translation="MTVQQARETLRAARLRSTTSRVAVLQHVAAAGKPVSHAEAADTL
                     VPQGFDKSTIYRCLVELADAGLLGRLDAGDHAWRFELRQAGVQHSTSEHPHFVCVDCG
                     KVTCLPDVDVKISTTKGAKSGPLGDVTEVYLKGHCKECL"
     gene            complement(2110..>2606)
                     /locus_tag="VFB80_07075"
     CDS             complement(2110..>2606)
                     /locus_tag="VFB80_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33562.1"
                     /translation="PGLTVGKRAQTDYVGSAGNLTNGSDGPVTQSRNTGIPRVRMEMV
                     TDGTSNTLFAGEGCQAIQWYRSATPTPERDWHRGGYTVGWASGSNSHWWNRRGAFPPL
                     KDFYSTSTNSYILIMRSFGSAHPGGINAVLGDGSVRTISYTVNPQTFINLCVRNDGET
                     LGDY"
BASE COUNT          422 a          861 c          885 g          438 t
ORIGIN      
        1 ctaaagaaac cccgcagcag cgagcccacc gcgaggccgc cgagaaggcc gagcgccgcc
       61 gcctgcacat gctccgcctc gaagaatgcc gcgaagccca gcgcaagggc ctccccaccc
      121 tcttcgtctt cgaccccgaa gacggccccc tgcccccgcg gggaggctgg ttctagggtg
      181 agccccacgc agcaagtagg gtgggctatg cccaccacag cgccgcggtg ggcacagccc
      241 accctacgcg atgggactga tgggaatccc agtttccact ttgccttttg catttctcaa
      301 tttgcaattt gcaatctctc gtgatcgcca ggcgccgttt cacgcggcag ccgccccatg
      361 gcgccgggcg cttcgctgct gcaccccact gcctccgacg ctggcagcgt cggctacgac
      421 cgccagccgc tgccgaccca gcggagggcg gagcggtcgg ccaggagcgg gtcgcagcgc
      481 cgcaggtagg cgctgagctg gccggtgtgg tgctggatgt ggcggaggct gatcaggtgc
      541 agttcgccgc gagagagcgg ataccaggag aacccggacg gcccggcgag cgactcttgc
      601 gtctcggcgg cgagggcctg ggccgccttc tgccggcaga ggctgacgta ggccagcgtc
      661 tcgtcctgcg gcaggccggg ggtgaaccgc ggctcgcgct cgtcgccgcc cgtcgcgtgc
      721 aggtcgcgga gcacgaacgc ctgctcgctc ggcgacaggt agaggtccac gaagaacagc
      781 gtgtgatagg ccacctgccg gaacgtgatg ccggcgatct tgccctccca gagttgcggc
      841 tggcacttct ggacgcactg gtggagcatg cacaggcccg cctcgaactg ggccgcgagg
      901 acctgccgga cgtagtcgat catgagttcg ctcctgactg ctctcgccga attcgcatcg
      961 tagccgaatt cgcatcgtag ccgaattcgc cagaattcgg tctcgcgggc gctatcgaac
     1021 tctggcgagt tcggctacga cgtacttcat gctcgatcgc ggaaaactgg cggtgctgtc
     1081 gatgttcgcc ctggcggtgg tcgcggcgac gtttgcctgg tggtggaact accgccgcgg
     1141 cgagcgctgc ctggccttct acggcagcga agcggcgacg ttgatccgca ccgcccagac
     1201 ggtcgagatc gtcgagctcg atccggcggc cggcgacctg gcgatctcgc ggcggatcga
     1261 catcagccga gcgccgggcc tgctcaacgc ccgcgcggcg ctgctggacg acgccagctt
     1321 cgactggagc gcgcctccgc cagagcccgc gagcggcacg cggctggtcc gctttgccga
     1381 gggcgatcgc gaggtgctgc tgcggttgga cagcgcgcgg cgctcgctgg agatcgttcc
     1441 cgccggcaag tcggcggtgc tggaccagaa aacatccgac ggctggcggc agtttttgga
     1501 aaggtacccg tagcggcggc ccggcctctc aaagccgcga ccagtggtcg cggtgcactg
     1561 gcagaccgcg accactggtc gcggctttgg ggacctacag gcactccttg cagtgcccct
     1621 tgaggtagac ctcggtgacg tcgccgaggg ggccgctttt ggcgcccttg gtggtcgaga
     1681 tcttgacgtc cacgtcgggc aggcaggtga ccttgccgca atcgacgcag acgaagtgcg
     1741 ggtgctcgct ggtggaatgc tgcaccccgg cctgccgcaa ttcgaaccgc caggcgtggt
     1801 cgccggcgtc gaggcggccg agcagccccg cgtcggccag ctccacgagg cagcggtaga
     1861 tcgtcgattt gtcgaaaccc tgcggcacca gggtgtcggc cgcttccgca tggctgaccg
     1921 gcttgccggc cgccgccacg tgctgcagca ccgccacccg cgaagtcgtg ctccgcagcc
     1981 gggccgcgcg cagcgtctcg cgggcttgct gaaccgtcat tgccgatggc gcactgctca
     2041 tgatcaagca gcatcatacc accgggcggc gaaattcaga atgcagagat cagaatgcag
     2101 aatgcggaac taatagtcgc ccagcgtttc gccgtcgttg cggacgcaaa ggttgataaa
     2161 tgtttgcgga ttgaccgtgt agctgatcgt tcgcaccgag ccgtcgccca ggaccgcgtt
     2221 aatgcctcct ggatgcgcgg agccgaagct gcgcatgatc aagatgtagc tgttcgtgga
     2281 cgtcgaatag aagtccttga gcggcggaaa cgcgccgcgg cgattccacc agtgcgagtt
     2341 gctgccggag gcccagccga ccgtgtaccc gccgcgatgc caatctcgtt cgggcgtggg
     2401 agtcgccgaa cgataccact ggatggcctg gcagccctcg ccggcgaaaa gcgtgttgga
     2461 ggttccgtcg gtcaccattt ccatccgcac tctgggaatg cccgtattgc gagattgcgt
     2521 cacgggaccg tccgagccgt tggtcaagtt gcccgccgag cctacatagt cggtctgggc
     2581 gcgcttgccg accgtcaggc cgggaa
//
LOCUS       DASRJB010000263         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2042281, whole genome shotgun sequence.
ACCESSION   DASRJB010000263 DASRJB010000000
VERSION     DASRJB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042281"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFB80_07080"
     CDS             complement(<1..458)
                     /locus_tag="VFB80_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33563.1"
                     /translation="MSAVLCIALLAASPQERGELANFIAGVERTPLLANGNPRKRVGR
                     EAGEVVWLHLEGMTLAADDFRLIGSLPSLRRLSLLRTNTTDKDLVHLRGLSKLEAIVL
                     SSTEVSDKAIDELTGLPALRTCCLGNVLITPAGIAQLKAHFPKLSLGYSER"
     gene            complement(492..1598)
                     /locus_tag="VFB80_07085"
     CDS             complement(492..1598)
                     /locus_tag="VFB80_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN33564.1"
                     /translation="MECSQRLQQGTWLLRECLPADVAPRTIALRSFPFLVGRLPACAL
                     CIPAANVSKEHAQIVQEAGRLYVHDLGSTNGTFVNQRRVSGREPLHDGDTLYFASEAF
                     RLQRQADDAHLRTVAEGSFVIVESLCQFDRLMSERAVVPHFQPIVQLAGGLVIGYEML
                     ARGSLSGLEGPGAMFQAAQRLGQEVALSKLLRREGVLAGRKLPGRPRLYLNTHPAEIA
                     APDLLPSLKEIRQLAPDQPMTIEVHEAAVAQIDDLLQLRESCRGLGMQLAYDDFGAGQ
                     ARLDELARVAPDCVKFDMRLIRDLHQAPAERRLVTERLVSLVRELGIVPLAEGVENQA
                     EADACRQIGFELGQGYFFGRPAPLSSLQAACGIG"
     gene            1852..>2100
                     /locus_tag="VFB80_07090"
     CDS             1852..>2100
                     /locus_tag="VFB80_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33565.1"
                     /translation="MFRSIACALIGLGCVTSGLAADKLPLALSASAGSRERVDALVQF
                     PLSKELAAAGPLRLMETTGGNETPIAAQVDPLGNRLWWV"
BASE COUNT          333 a          710 c          725 g          332 t
ORIGIN      
        1 cgttcggaat atcccaggct cagcttcggg aagtgcgcct tgagctgcgc gatgcccgca
       61 ggcgtgatga gcacgttgcc caggcagcaa gtgcgcaggg ccggcagccc cgtcagctcg
      121 tcgatcgcct tgtcgctcac ctccgtgctg gaaagcacga tcgcctcgag cttcgacagc
      181 ccgcggaggt gaaccaggtc cttgtcggtg gtgttggtgc ggagcaggct cagccgccgc
      241 agcgacggca aggagccgat caggcgaaaa tcgtccgccg cgagcgtcat gccctccagg
      301 tgcagccaca cgacttcgcc ggcctcgcgt cccacgcgct tccgtggatt gccgttcgcc
      361 aggagcggtg tccgctcgac gccggcgata aagttggcca attcgccgcg ctcctggggg
      421 ctggcagcca agagcgcgat acagagaact gcggacatgt tccgctcctt gaattcgacg
      481 actcattcaa actagccgat gccgcaggcg gcttgcaagc tcgacagagg cgcgggtcgt
      541 ccgaagaagt agccctggcc cagctcgaag ccaatctggc ggcaggcgtc ggcctcggcc
      601 tggttttcca cgccttcggc cagcggcacg atgcccagct cgcggaccag cgagaccagc
      661 cgctcggtga ccagccgccg ctcggccggg gcctggtgca ggtcgcggat caatcgcatg
      721 tcgaatttca cgcagtcggg ggccacgcgg gccaactcgt cgagccgcgc ctggcccgcg
      781 ccgaagtcgt cgtaggcgag ctgcataccc aggccgcggc aggattcccg cagctgcagc
      841 aggtcgtcga tttgcgccac tgccgcctcg tggacttcga tcgtcatcgg ctggtcgggc
      901 gcgagctggc ggatttcctt cagcgacggc agcaaatccg gcgcggcgat ttccgccggg
      961 tgcgtattga ggtacagccg cgggcggccg ggcagcttgc ggccggcgag cacgccctcg
     1021 cggcggagca gcttgctgag cgcgacttcc tgcccgagcc gctgcgccgc ctggaacatg
     1081 gccccgggac cttccagccc ggacaggctg ccgcgggcca gcatctcgta gccgatgacc
     1141 aggccgccgg ccagctgcac gatcggctgg aagtgcggaa cgacggcccg ctcgctcatc
     1201 agccggtcga actggcacag cgattcgacg atcacgaacg agccctcggc cacggtccgc
     1261 aggtgggcgt cgtcggcctg ccgctgcagg cgaaaggcct cgctggcgaa atacagcgtg
     1321 tcgccgtcgt gcagcggctc gcggccgctc acgcggcgct ggttcacgaa cgtcccattc
     1381 gtgctgccca aatcgtgaac gtacagccgg cccgcttcct gcacgatctg ggcgtgctcc
     1441 ttcgagacgt tggccgcggg aatgcacagg gcgcacgcgg gcagccggcc gacgaggaac
     1501 ggaaacgagc ggagggcgat cgtccgcggc gctacgtcgg ccggcaggca ttcgcggagg
     1561 agccacgtgc cctgttgtag tcgctgcgag cactccatcg atggatgccg gcaagaggag
     1621 cctggccgcc gcccgaggca ggcgcggcgc ggaaaatcac gagaaaccgt agcttggccc
     1681 ccacgcttgg gatgccggtc ccgccgaggt atgcggccgc ccgcaaagtg cccggtggcc
     1741 ttaatccgat tgcaccggct cttcctttcc ggccgccgcg cggcgacagt aggccctatg
     1801 cttcggccgc agcggtccgg gcgcctgttt ttagctacgg gagcagccaa catgtttcgg
     1861 tccattgcct gtgcactgat cggcctgggt tgtgtgacca gcggactggc ggccgacaag
     1921 ctcccgctgg ctttgagcgc cagcgccggg tcgcgagagc gagtcgatgc gctggtgcag
     1981 tttccactgt ccaaggagct ggcggcggcc gggccgctgc gtctgatgga aacgaccggc
     2041 ggaaacgaga cgcccatcgc ggcccaggtc gatccgctgg gcaaccggct atggtgggtc
//
LOCUS       DASRJB010000264        10306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_637844, whole genome shotgun sequence.
ACCESSION   DASRJB010000264 DASRJB010000000
VERSION     DASRJB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637844"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2378)
                     /locus_tag="VFB80_07095"
     CDS             complement(189..2378)
                     /locus_tag="VFB80_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN33566.1"
                     /translation="MGKQIAAFSLQDFRGKSWSLDDFKASKGLAVVFLGTECPIAGQY
                     GARLQALAAKYADAGIAFLAIDANQQDSITELAHFARIHKLDIPVLKDAGNKVADQFG
                     AQRTPEAFLLDASRKVVFQGRIDDQFTYGIQRTKAEKSYLTDAIDQLVAGQPISVAYA
                     EPVGCHIGRVLAPQAGSDVTYSKQIARILQDNCVKCHRPGEIGPFSLTNYDEVVGWAE
                     MIREVVQEQRMPPWHANPQFGHFSNDSRLSNADKEAIYKWVDAGAPEGDKRDLPPPKQ
                     YVEGWQIGQPDQVVYISEKPYKVPATGTVRYQYFIVDPGFTEDKWIKAGECRPGSRAV
                     VHHIIVGVVPPGFTPGGGRGPGEAGFSDWLVATAPGARPMMLPDGMAKFVPAGSKLVF
                     QVHYTPNGTPQEDRSCVGLIFTDGKTVKQKVATDKAANPRFLIPPGADNHKVEANHAF
                     GNDTLVLALFPHMHLRGKAFRYTAIFADGKQEVLLDVPHYDFNWQNSYVLTEPKLMPK
                     GSRMFCEAWFDNSEGNLANPDPKATVRWGDQTWEEMMIGYFSSTPAQQDRQASPAQTR
                     RTQQFVALVKEGKAALSDALKAQAKEALASDANLNKFGPELRKIAPQLDRLCWTTVEG
                     DKLVIRRCVQEESFTQQVGGAGRKVDVRITKLATYADAAEPVVNQQLAAVRAIDMTFM
                     ARAFGSSLHIPVKIDGVSGTLNFWSAEKDAFPPELVALLTELARSMQ"
     gene            complement(2588..4846)
                     /gene="feoB"
                     /locus_tag="VFB80_07100"
     CDS             complement(2588..4846)
                     /gene="feoB"
                     /locus_tag="VFB80_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZN33567.1"
                     /translation="MTASLAAPPKPLTVALVGNPNTGKSTLFSRLAGVRQRVGNYPGV
                     TVEKKLGQAEFHGQRFTLVDLPGTYSLAPRSPDEMVAVDVLLGRCDSGSGLRGDVPPI
                     DVVLCIVDASNLERNFYLLSQVLELGLPTVVALNMVDVAKGKGLTLDLKKLAERLPAK
                     FVPVQANKGVGVSELKQALAAAASKPPVPVPSPFPEKFVSEVEALHQRAGCNGHPRLP
                     RYLVERLLLDTTGYLAAAGLPGISRELLQEVPAARERLAAAGLAVPAVEALARYQWAS
                     QVLSGIVARPAHKPVTLSDRIDRVLTHRVWGTLVFVLLMLLLFQAVYFVPDMSGANWL
                     LEKLMALAASAVRAIVPAGALQSLLTTGVIGGVGGVVVFLPQILTLFFFIALLEDCGY
                     MARAAYLMDKLMSRIGLSGKSFIPLLSSFACAVPGIMATRVIENRRDRLVTILVAPLM
                     SCSARLPVYTLLIAAFVPNLTIGAGWFALPWLHGLVMFAMYSIGIVTGIAAAWIFKKT
                     LFRGSTPPFVMELPSYKLPDVRVVLHRMLEQGWAFLSRAGTLIVAVSIVVWGLAYWPH
                     DRSQISPVLFDKQANLQAQIEAAPEDQRKALKVNLATVENQIAGQYLQHSFLGRAGKL
                     IEPAVKPLGWDWRIGCAAIASFPAREVVVATLGVIYNLGDEQDETSDDLKQTLKDATW
                     DGTNRPVFTLPVALSVMVFFALCAQCSSTLVIMRRETGSWRWPAFTFAYMTTLAYLGA
                     LATYKIGTWLSG"
     gene            complement(4850..5089)
                     /locus_tag="VFB80_07105"
     CDS             complement(4850..5089)
                     /locus_tag="VFB80_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HZN33568.1"
                     /translation="MTSVADLRVGARATVLRIEGVDEVSLRLMEMGLTPGVDVALLGV
                     APLGDPLELEVRGYRLSLRKKEAARVEVNLVTNDK"
     gene            complement(5086..5322)
                     /locus_tag="VFB80_07110"
     CDS             complement(5086..5322)
                     /locus_tag="VFB80_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZN33569.1"
                     /translation="MPDLLPLHLLACGQRARIDQVLGCPDHVHRLQELGMRIGCSIEM
                     VQAGSPCIVKLDGNRLAFRDNEMLSVLVRLGELS"
     gene            complement(5467..7071)
                     /locus_tag="VFB80_07115"
     CDS             complement(5467..7071)
                     /locus_tag="VFB80_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZN33570.1"
                     /translation="MPQPSTSPLDWSATEIAQRIAGRQVSATEVAKAFIARIEEVNPA
                     LNAVVIPRFEQALAEAAAADQQQARGQPLGPLHGVPMTVKECFHVAGTKATIGLSRAD
                     YQQPATEDGVMVARLRRAGAIILGKTNLPQLMIWHECDNPVYGRTNNPWDLSRTPGGS
                     TGGEAAIIAARGSPLGLGNDLGGSIRVPCHFCGIHGFKPTSFRLTRKGTRHTLHGFEA
                     IVTQPGPMARQVADLWLMLQVLADNSNGDNPPDVPPAQLGDPSQIDVAALRVAVWTDD
                     RVFPPSAAVQRAVREAAAALAARGATIVELNPAEMCAPLSMDEMFATYCSLIGSDGGA
                     GARRLAADSKLDWRVARLIWLAGLGPVRRSAVLSSLKLTGQKWMARLVSAARPVSADQ
                     FWQLSFQRLEFAARFTERLRSRGIDAILCPPHALPATPHLAGFDLIAAASYSMLFNFI
                     GFPAGVVSTTRVRPDEAAGRWNDRDVVLRQAAATDEGSAGLPIGVQVAALPWRDDIVL
                     AVMGAIETSLRTKQDWPIHELAVTKA"
     gene            complement(7141..8334)
                     /locus_tag="VFB80_07120"
     CDS             complement(7141..8334)
                     /locus_tag="VFB80_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZN33571.1"
                     /translation="MADSADAVEALKAAGARLNPDAAGNVIEVVLDADTGSDEQLTHL
                     KGLPNVRVLAAEARGVTDKGLAELKGHPNLRNLKLERALVTNGGMALLRDIPKLEDLD
                     LKRTDITAAGYQHVGQIKTLKRLRAVYTAFDDACCEGIKDLHNLELIDLQDCKYVTDK
                     GLAVLQGMPKLRNVRLYGDPGINDKVLGYLADAKDLKVLSLHQSAVTEAGLEKISKLK
                     NLEELLLYGAKHINDAAVAKIGGHSKLKVLELRETPISSLAISYLKDLKDLRELDLSQ
                     TASVGDEGLEHLQGLTNLESLNLWSCQLTDAGLAHVAKLKKLKTLNLDNCYVTDEGLK
                     HILELPELEDLNLSHTKITDAGLAQLKSLKKLTTLDVSAVPTISEEAVETLKKELPKL
                     EDVRL"
     gene            8736..10217
                     /locus_tag="VFB80_07125"
     CDS             8736..10217
                     /locus_tag="VFB80_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN33572.1"
                     /translation="MATAVASAKPTTPRPRIHQTQCFIGGQWVPSQSGKTFQTLDPAN
                     EEVIAEVAEGDAADVDLAVKAARKQFDGGEWSKLDARDRGRLINKLADLIEEEAAELA
                     ALESLDNGKPIRDSKAADLPLTIDALRYYAGYADKIHGQTIPVRGNYFTYTRKEPVGV
                     VGQIIPWNFPMLMVAWKWGPALAAGCTIVMKPAEQTPLTCLRMARLAQKAGIPDGVIN
                     VVPGYGPTAGGAIVKHPGIDKIAFTGSGETAQIIMRDAAPTLKRITFELGGKSPNIVF
                     SDADIDAAIAGAHFGLYFNQGQCCCAGSRLFVEEKVYDTFVDRLAGMNKGRKLGDPLD
                     PETEQGPQVDKAQFDKVMHYIDLGNKEGAKCLTGGKRFGDRGYYIEPTLFVDVKDEMA
                     IAKDEIFGPVMSVLKFKSLDEIAARANNTTYGLAAAVWTRDIAKAHALAAKLRAGTVW
                     INCYDVFDAAAPFGGYKMSGMGRELGEEGLKAYTETKTVTVAL"
BASE COUNT         1839 a         3396 c         3261 g         1810 t
ORIGIN      
        1 cggccacacc tgccacggcg agccaatggc cagtgacaat tgcagcccgc tgtggaggca
       61 gcgtccgcag ccgccaggct gacagccccg cgacgatgca gacgacggtc atcgcaccca
      121 gtaggtgacg cgtaccaagc tgcccgggcc gaaacgcttc gtcttcgggc tgcctggcca
      181 ttttccgctc actgcatcga cctggccagc tcggtgagca gcgcgaccag ctccggcgga
      241 aaggcgtcct tctcggcgct ccagaagttg agcgttccgc tgacgccatc gatctttacg
      301 ggaatgtgca agctcgagcc gaaggcccgg gccatgaacg tcatgtcgat cgcgcggacg
      361 gcagcgagtt gctggttgac gaccggctcg gccgcgtccg cataggtcgc cagctttgta
      421 atccgcacat ccaccttgcg gcctgcgccg ccgacctgct gcgtgaagct ctcctcctgc
      481 acgcagcggc gaatcacgag cttgtcgcct tccaccgtcg tccagcacag ccggtccagc
      541 tgcggcgcga tcttccgcag ctcggggccg aacttgttca agttggcgtc cgaggccagc
      601 gcctccttgg cctgagcttt cagcgcgtcc gacagcgcgg ccttcccttc tttcaccagg
      661 gccacgaact gctgcgtccg ccgcgtctgc gccggtgagg cctgtcggtc ttgctgggcc
      721 ggcgtggacg agaaatagcc gatcatcatc tcttcccagg tctggtcccc ccagcggacg
      781 gtcgccttcg gatcgggatt ggccaggttc ccctccgagt tatcgaacca ggcctcgcag
      841 aacatccgcg agcccttggg catcagcttc ggctcggtca gcacatagga gttctgccag
      901 ttgaagtcgt aatgcggcac gtcgagcagc acttcctgct tgccgtcggc aaagatcgcg
      961 gtatagcgaa aagccttgcc ccgcagatgc atgtgcggaa acagggccag caccagcgtg
     1021 tcattgccga aggcgtggtt cgcctcgacc ttgtggttat ccgcgccggg cggaatcagg
     1081 aaccgcggat tggcggcctt gtcggtcgcc accttctgct tcaccgtctt gccgtcggtg
     1141 aagatcaggc ccacgcaact acggtcctcc tgaggagtgc cattgggcgt gtagtgcacc
     1201 tggaacacga gcttcgaacc ggcagggaca aacttcgcca tcccatcggg cagcatcatc
     1261 ggccgcgccc ccggcgcggt tgccacgagc caatccgaaa accccgcctc gcccggcccc
     1321 cgcccgccgc ccggcgtgaa gcccggcggc acaacgccca cgatgatgtg gtgcacgacc
     1381 gcgcggctac ccggccggca ctcgcccgct ttgatccact tgtcctcggt gaagcccgga
     1441 tcgacgatga aatactgata ccggaccgtg cctgtcgccg gaaccttgta cggcttctcg
     1501 gagatataga ccacctggtc cggctggccg atctgccagc cttcgacata ttgcttcggc
     1561 gggggaaggt cgcgcttgtc gccttcggga gcgccggcat cgacccactt gtagatcgcc
     1621 tctttgtcgg cattgctcag gcgggagtcg tttgagaagt gcccgaactg cggattggcg
     1681 tgccacggcg gcatccgctg ctcctggacc acttcgcgga tcatttccgc ccagccgacg
     1741 acctcgtcat agttggtcag cgagaatggc ccgatctcgc ccggccggtg gcacttcacg
     1801 cagttgtcct gcaggatgcg ggcgatctgc ttcgagtagg tcacgtcgct gccggcctgg
     1861 ggcgcgagca cgcggccgat gtggcagcca accggctcgg catacgccac ggaaatcggc
     1921 tggcccgcca cgagctggtc gatggcgtcg gtcaggtagg acttctcggc cttcgtccgc
     1981 tgaatgccgt aggtgaactg gtcgtcgatt cgcccctgaa acacgacctt tcgggaggca
     2041 tccaggagga aagcttctgg cgtccgctgc gccccgaact ggtcggcaac cttgttgccc
     2101 gcgtccttca gaaccggaat atccagcttg tgaatccggg caaagtgggc gagctccgtg
     2161 atcgagtcct gctggttggc atcgatggcc aggaaggcga ttccggcgtc ggcatacttg
     2221 gcggccaacg cctgcaatcg agccccgtac tgacccgcaa tcggacattc cgtgcctagg
     2281 aagacaacgg caagcccctt gctggccttg aagtcatcca gcgaccacga tttgccgcga
     2341 aaatcctgga gcgagaaggc ggcgatttgc ttgcccacgg agttcggcct cgcctcagcc
     2401 gccatagccc cggcagccaa ggaaattgag cacgcagcca aagcgccaaa ggaaaaacgc
     2461 cgcataagtt tgccgacttc ctttcactcc ggtggatgtg gacgcaccgc gacccgcggg
     2521 acgtttcgtc gttctataaa ccccagttaa cgcccgcggg tttcgttcaa acgttgatcg
     2581 gcgagaatca gcctgacagc caggtgccga ttttgtacgt cgccagggct ccaagatagg
     2641 ccagagtggt catgtaggca aaggtaaatg ccggccaccg ccagctgccg gtttcgcggc
     2701 gcatgatgac gagcgtcgag gagcactggg cgcagagggc gaagaacacc atcacggaga
     2761 gggccacggg gagcgtaaat acgggccggt tggttccatc ccaagtggca tccttcaggg
     2821 tctgcttcag gtcgtcggac gtttcgtcct gctcgtcccc caggttgtag atgacgccca
     2881 gggtcgccac gacgacttcg cgggccggga acgaggcgat ggccgcacag ccgatcctcc
     2941 agtcccagcc gagcggcttg acggccggct cgatcagctt gccagcccgg cccaggaagc
     3001 tgtgctgaag gtactggccg gcgatctggt tttcgacggt ggccaggttg actttgagcg
     3061 ctttgcgctg gtcttcgggc gcagcttcga tttgtgcctg caagttggct tgcttatcaa
     3121 acaaaacggg actgatctgt gagcggtcgt gcggccagta ggccaggccc caaacgacga
     3181 tcgacacggc gacaatcagc gtgcccgccc gcgacaggaa ggcccagccc tgctccagca
     3241 tccggtgcag cacgactcgc acatcgggca gcttgtagct cggcagttcc atgacgaacg
     3301 gcggcgtgct tccgcgaaag agcgtcttct tgaaaatcca ggcggccgca atgccggtga
     3361 cgatgccgat cgagtacatc gcaaacatca ccagtccgtg cagccacggc agcgcgaacc
     3421 agccggcgcc gatcgtaagg ttgggcacga acgcggcgat caggagcgta tagaccggca
     3481 ggcgggcgct gcagctcatc agcggcgcga caaggatcgt caccagccgg tcgcggcggt
     3541 tttcgatcac gcgggttgcc atgatccccg gcacggcgca cgcgaacgat gagagcagcg
     3601 gaatgaacga cttgcccgac aggccgatcc gcgacatcag cttgtccatc aggtacgccg
     3661 cgcgggccat gtagccgcag tcttcgagca gcgcgatgaa gaagaacaga gtcagaatct
     3721 gcggcaggaa cacgaccacg ccgccgacgc cgccgatgac gcccgtagtg agcagcgatt
     3781 gcagcgctcc cgcgggcacg atcgcccgca cagcgctggc ggctagcgcc atgagctttt
     3841 cgagcagcca atttgcgccc gacatgtccg gcacgaaata gacggcctga aacagcagca
     3901 gcatcagcag cacaaagacc agcgtgcccc acactcggtg cgtcagcacg cggtcgatcc
     3961 ggtcgctgag cgtcaccggc ttgtgcgcgg gtcgcgccac gatgccggaa agcacttgag
     4021 atgcccactg atagcgggcc aaggcctcaa ccgcgggcac cgccagaccg gccgcggcca
     4081 gccgctcgcg ggccgccggc acttcctgga gcagttcacg gctgattccc ggcagcccag
     4141 cggctgccag gtagccggtc gtatccagca gcagccgctc gaccagatac cgcggcagcc
     4201 gtggatggcc gttgcagccc gcccgctggt gcagcgcctc gacttcgctc acgaattttt
     4261 cgggaaaggg gctcggcacg ggcacgggcg gcttgctcgc ggcggcggcc aacgcctgct
     4321 tcagctcgct gacgccgacg cctttgttcg cttgcacggg tacgaatttc gcgggcagcc
     4381 gctcggccag ctttttcaga tcgagcgtca gccctttgcc cttggccaca tcgaccatgt
     4441 tgagcgcgac aacggtcggc agtcccagct ccagcacctg gctgagcagg tagaaattcc
     4501 gctccaggtt gctggcatcg acgatgcaca gcacgacatc gatcggcggc acgtcgccgc
     4561 gcagcccgct gccagaatca cagcggccga gcagcacatc gaccgcgacc atctcgtcgg
     4621 gcgatcgcgg ggcgaggctg tacgttcccg gcagatcgac cagcgtgaag cgctgcccgt
     4681 gaaactcggc ctgtccgagc tttttttcga cagtcacgcc cggatagttg cccactcgct
     4741 gccgcacgcc ggccagtcgg ctaaacagcg tcgatttacc cgtattggga ttgcccacca
     4801 gggcgacggt cagcggcttg ggcggcgcgg cgagtgacgc ggtcattggt cacttgtcat
     4861 tcgtcactag gttcacttca acccgggccg cttccttttt gcggagactc agtcgatagc
     4921 cgcggacttc caactccagc gggtcgccca gcggtgcaac gccaagcagg gcaacgtcaa
     4981 ctccaggcgt taatcccatc tccatcaggc ggaggctgac ttcatcgacg ccttcgatcc
     5041 gcagcaccgt ggcccgcgca cccacgcgca aatcggccac ggaggtcacg acaactctcc
     5101 cagtcgcacc agcacgctca gcatttcgtt gtcgcggaac gccagccgat tgccgtcgag
     5161 ttttacaatg cacgggctgc cggcttggac catctctatc gagcagccga tgcgcatgcc
     5221 cagttcctgg aggcggtgga cgtgatcggg acatcccagg acctgatcga tgcgtgcgcg
     5281 ctggccgcaa gcaagcagat ggaggggaag cagatccggc atgggagggc ggctaaatga
     5341 aactgagtct cactagcatc atagggaatg agctcgcccg ttacaactgt gccgggagaa
     5401 atctgcctgc cgccgaccca atacccccag tatgggcttg tctagacgca atatcgagcc
     5461 attgcctcac gcctttgtga cggccagctc gtggatgggc cagtcttgct tcgttcgtag
     5521 cgaagtctcg atcgctccca tcacggccaa gacgatgtca tctcgccacg gcaaggcggc
     5581 cacctgcacg ccaatgggca agcccgcgct cccctcgtcg gtggccgccg cctgccgcag
     5641 gaccacatcg cgatcgttcc atcggccggc tgcctcatcg ggccgcacgc gggtcgtcga
     5701 gacgactccg gccggaaagc cgatgaaatt gaacagcatc gagtagctcg ctgcggcgat
     5761 caaatcgaac cccgcgaggt gcggcgtggc cggcagagcg tgcggcggac agaggatcgc
     5821 atcgattcca cggctgcgca gcctttccgt gaatcgcgca gcgaactcaa gcctttgaaa
     5881 gctgagttgc caaaactggt ccgccgagac cggccgggcg gcactgacga gtctggccat
     5941 ccacttctgg cccgtgagct tgagcgagct gagcacggcg ctgcgccgca ccggcccgag
     6001 gccggccagc cagatcagtc gggccacgcg ccaatcgagc ttgctgtcgg ctgccagtcg
     6061 ccgcgcgccg gcgccgccgt cggagccgat caagctgcaa taggtcgcga acatttcgtc
     6121 catcgacagc ggggcgcaca tctccgccgg gttgagctcg acaatcgtcg cgccccgcgc
     6181 ggccagcgct gcggccgctt cacggacggc acgctgcacg gcggccgaag gcggaaagac
     6241 ccggtcgtcg gtccagaccg cgacgcggag agcggcgaca tcgatctgcg acggatcgcc
     6301 gagctgagcc ggcggcacat ccggcgggtt gtcgccgttg gagttatcgg ccagcacttg
     6361 caacatcagc cacaggtcgg cgacttgccg ggccatcggt ccgggctgcg tcacgatggc
     6421 ctcgaatccg tgcagcgtgt gccgcgtgcc cttccgggtc aggcggaacg acgtcggctt
     6481 gaagccgtgg attccgcaaa agtggcaggg cacgcggatg ctgccgccca ggtcgttgcc
     6541 cagacccagc ggcgagccgc gggcggcgat gatggccgct tcgccgccgg tgctgccgcc
     6601 aggagtgcgg gacaggtccc agggattgtt cgtgcggccg tacaccggat tgtcgcactc
     6661 gtgccagatc atcaactgcg gcaggttggt cttgccgagg atgatcgccc cggcccgccg
     6721 cagacgggcc accatcacgc cgtcttcggt tgcgggctgc tggtagtcgg cccgggaaag
     6781 tccgatcgtg gccttggtgc cggcgacgtg aaagcactct ttgaccgtca tcggcacgcc
     6841 gtgcagcggg ccgagcggct ggccgcgggc ctgctgctgg tcggctgccg cggcttcagc
     6901 tagagcttgt tcgaaccgcg ggatgacgac cgcgttgagc gcaggattaa cctcctcgat
     6961 ccgggcgata aaggccttgg caacctcggt cgccgagact tgccgccctg cgatccgctg
     7021 cgcgatctcg gtcgcggacc agtcgagcgg cgaagttgag ggctgtggca ctattgagtg
     7081 gggcagacat tcctgtctgc cagagaagac atggcagaca agaatgtctg ccccacctca
     7141 ctacagccgc acgtcttcca gcttcggcag ctctttcttc agcgtttcga ccgcctcctc
     7201 ggagatcgtg ggcacggccg agacgtcgag cgtggtcaac ttcttcaggc tcttgagctg
     7261 cgcgaggccg gcgtccgtga tcttcgtgtg cgaaagattg aggtcttcga gctcgggcag
     7321 ctccaggatg tgcttcaggc cttcgtcggt gacgtagcag ttgtcgaggt tgagcgtttt
     7381 cagctttttc agcttcgcga cgtgagccag ccctgcatcg gtcaactggc acgaccacag
     7441 gttgaggctt tccaggttcg tcagcccctg cagatgctcc aggccctcgt cgccgacgct
     7501 ggccgtttgc gatagatcca gctcgcgcag gtccttcagg tccttgaggt acgagatcgc
     7561 caggctggag atcggcgtct cgcgcagctc cagcactttg agcttcgagt ggccgccgat
     7621 cttggccacg gcagcgtcgt tgatgtgctt cgctccatag agcagcagct cttccaggtt
     7681 cttcaacttc gagatcttct ccagcccggc ctcggtgacg gccgactggt gcagcgacag
     7741 gaccttcaga tccttggcgt ccgcgaggta gcccagcacc ttgtcattga ttcccggatc
     7801 gccatagagc cgcacgttgc ggagcttggg cattccctgg agcaccgcca ggcccttgtc
     7861 ggtgacgtat ttgcagtctt gcagatcgat cagctccagg ttgtgcaggt ccttgatccc
     7921 ttcgcagcag gcgtcgtcga acgccgtata gacggcccgc agccgcttga gcgtcttgat
     7981 ctggccgacg tgctgatagc ctgcggcggt gatgtcggtc cgcttgaggt cgaggtcctc
     8041 cagcttggga atgtcccgga gcagcgccat tccgccgttg gtgacgagcg ctcgctcgag
     8101 cttcaggttc cgcaggttgg gatgcccctt gagctcggcg agtcccttgt cggtgacgcc
     8161 gcgcgcctcg gccgccaaca cgcgcacgtt gggcaggccc ttgaggtgcg tcagctgctc
     8221 gtcgctgccg gtgtcggcgt cgagaaccac ttcgatcacg ttgcccgcag catccggatt
     8281 cagtctcgcc ccggcagctt tcagcgcctc gaccgcatcg gcgctgtccg ccacagccgc
     8341 ctcgccgccg gtcgacgtgc tggacccgcc atcggttgcc acacctccgc cagctccccc
     8401 tccgcccacc ggcgcaggcg gcagcgggga agggcagccc gcacaaataa cgagtaaggc
     8461 aaccccgaag ggcaaacgga atgcgcgggg ggtgggcatt gcgaatttcc ttgcgaaaaa
     8521 acagcctatc gcgggccgcc tttgcacgac aggggactcc cgaccgcaat ccagctcttc
     8581 gatccacttt gcgagctttg cctcttcgcg gcttggcgtt gaatcgaaga ccgttgccgt
     8641 cgggtcttgg tcaccatcgc ctaaggcgct tgtccctttt cgagcccgag cgataaaatt
     8701 caattggctt cttctactct ccctggagca ttctcatggc cacggctgtt gcctctgcga
     8761 aaccgaccac gcctcgcccg cgtattcacc agacccagtg cttcatcggc gggcagtggg
     8821 ttccctccca gagcggcaag acgtttcaga cgctcgaccc ggcgaacgag gaagtcatcg
     8881 ccgaagtcgc cgagggagac gcggcggatg tcgatctggc cgtgaaagcc gcccgcaagc
     8941 agttcgacgg cggcgagtgg agcaagctcg atgcccgcga ccgcggccgg ctgatcaaca
     9001 agctggcgga cctgatcgag gaagaggcgg cggagctcgc cgcgctggaa tcgctcgaca
     9061 acggcaagcc gatccgcgac agcaaagcgg ccgacctgcc gctgacgatc gatgccttgc
     9121 gctactacgc cggctacgcc gacaagatcc acggccagac gatccctgtc cgcggcaact
     9181 acttcaccta cacgcggaaa gagccggtgg gcgtcgtcgg gcagatcatc ccctggaact
     9241 tccccatgct gatggtggcc tggaagtggg ggccggccct ggccgccggc tgcacgatcg
     9301 tgatgaagcc ggccgagcag acgccgctga cctgccttcg catggcccgc ctggcccaga
     9361 aagccggcat tcccgacggc gtcatcaacg tcgtccccgg ctacgggccg accgccggcg
     9421 gggcgatcgt caagcatccg gggatcgaca agattgcctt caccggaagc ggcgaaacgg
     9481 cgcagatcat catgcgcgac gccgcgccga cgctgaagcg aatcacgttc gagttgggcg
     9541 gaaagagccc gaacatcgtc ttctccgacg ccgacatcga cgcggcgatc gcgggagccc
     9601 acttcggcct ctacttcaac caaggccagt gctgctgcgc cggcagccgg ctgttcgtcg
     9661 aggagaaggt ctatgacacg ttcgtcgatc ggctggcggg aatgaacaag ggccgcaagc
     9721 tgggcgaccc gctcgacccc gagaccgagc aaggcccgca ggtggataag gcccagttcg
     9781 acaaggtcat gcactacatc gacctgggga acaaagaagg cgccaagtgc ctcaccggcg
     9841 gcaagcggtt tggcgaccgc ggttactaca tcgagccgac gctgtttgtg gacgtgaaag
     9901 acgagatggc gattgccaag gacgagatct ttggcccggt gatgtcggtg ctgaagttca
     9961 agtcgctgga cgagatcgcg gcccgggcca acaacacgac gtacggcctg gccgccgccg
    10021 tctggacccg cgacatcgcc aaggcccacg ccctggccgc gaaactccgc gccggcaccg
    10081 tctggatcaa ctgctacgac gtcttcgacg ccgccgcgcc cttcggcggc tacaagatgt
    10141 ccggcatggg ccgcgagcta ggcgaagaag gcctcaaagc ctacaccgag accaagacgg
    10201 tcacggtggc gctgtgagga cgttagaacg atttcgtgtg gctgcagtgg tcgcgttggt
    10261 cgcggctggc tgccgggcga atagcgtgat ttcgcaaccg gagcca
//
LOCUS       DASRJB010000265         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3743179, whole genome shotgun sequence.
ACCESSION   DASRJB010000265 DASRJB010000000
VERSION     DASRJB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743179"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /locus_tag="VFB80_07130"
     CDS             complement(<1..2200)
                     /locus_tag="VFB80_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN33573.1"
                     /translation="MHRITFALTLLLPAVGVADEPLSVKDLAAKVRDSIVVIGFAGRE
                     GAQQGLGTGFVIDKGGLIATNLHVIGEARPISVQTAGGKTLTVKAVHASDRALDLAIV
                     EVDATDLRPLELSGAEKIDAGEPVVVMGNPQGLKHSVVSGVVSGTREIDGRSMLQLAI
                     PVEPGNSGGPVLDMQGRVLGIVTMKSLVTQNLGFAVAAADLKTLRDKPNPVPIDRWLT
                     IGGIDRTQWEPLFGARWQQRAGRLLVDGVGAGFGGRSLLLSKGDSPAVPYELAVQVKL
                     DDESGAAGLVFHADGGDKHYGFYPSNGKLRLSRFEGPDVFSWQVLAEKPSEHYRPGEW
                     NRLKVRVEKGKLRCFVNDELVIEAAEDAFAKGRIGLAKFRNTGAEFRRFAVGKELPGE
                     RPADDVRSKLAAAIDKLPPLAEAREAALADLASADSEPAQAALLAKAAELEARAADLK
                     RLAADVRTAAIAAEFTKVAGAEVQQIDLLRAALTIARLGDEDLDVAAYAAYVDRMAAE
                     IKHKLPAKATEADKLAALNEYLFKDNGFHGSRTDYYHRANSFLSRVIDDREGLPITLS
                     VLYIELGARLGLKFEGVGLPAHFVVRHVPAEGPPQLIDVFEGGLRLTREEAEKKIAAL
                     TGEPPLAEHFDAVTPRQILMRILSNLIGNAQNPRTGPDREALIRYESLMLVLDPTLVR
                     DRGMRAVCRWETGRTAAAVADLQVLLDAKPAGVDLDELQKMQEYFRTNKAP"
     gene            complement(2222..2830)
                     /locus_tag="VFB80_07135"
     CDS             complement(2222..2830)
                     /locus_tag="VFB80_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN33574.1"
                     /translation="MVRAVIAAAIVLLPWAVIAQEKFLGRTAEQWSQALTTTTGQQRV
                     HAAWALAQMAGRASGDASDHVQFAELVKLISDGDPSVRYWGALGLAAFAEHLPQGDGG
                     RAGAIGALVPLLDDKSPAPRIAAASALGQLGQSDRALRVLVAAMNDPQEATRIQAVAA
                     LEKLGPAARPAEATLRAARADSSEYVKRISTRALEKLTAAGK"
     gene            complement(2837..3199)
                     /locus_tag="VFB80_07140"
     CDS             complement(2837..3199)
                     /locus_tag="VFB80_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZN33575.1"
                     /translation="MLYGYIKNLSHDRGFGFIEPCSDGPDVYFHATTVGSQVFDGLQP
                     GQPVRFEIQKLTEKEREARRKLTTKQAPRAAKVELLQRMPGGVLPRPTQDIAPRHHPK
                     AKQRKATWKRKINLKPKE"
     gene            complement(3233..>4196)
                     /locus_tag="VFB80_07145"
     CDS             complement(3233..>4196)
                     /locus_tag="VFB80_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN33576.1"
                     /translation="ELRPLGNTGLQVSPVALGCWPISGMTSLGVNEVDSLATLHAALD
                     AGVNFFDTAYAYGAGGESERLITRAIAGRRGDVVIATKCGIEWNAHRERVIDGRPATL
                     RRQCEESLRRLNTDRVELLYLHAPDPETTLVDSAAELLRLKREGQTLAVGVSNFTLPQ
                     LEEFHAACPIEVFQPPYNMLQRDIERDTLPWCRERNIAVCVYWPLMKGLLAGRLPRDH
                     VFQPGDGRAKYPMFQGQEWQKNQDFLGELSAVAEEIGKTVAQVVVNWTIQQPGITAAL
                     CGAKRPQQIRETAGAMGWRLTRQQMARIAAALARRGVAVTRAAV"
BASE COUNT          624 a         1464 c         1396 g          712 t
ORIGIN      
        1 gcggggcctt gttcgtgcgg aaatattcct gcattttctg cagctcgtcc agatcgacgc
       61 cggcgggttt ggcgtcgagc agcacctgca ggtcggcaac ggcggcggcc gtgcggccgg
      121 tctcccagcg gcagacggcc cgcatgccgc ggtcgcggac gagcgtcgga tcgagcacga
      181 gcatcaggct ctcgtagcgg atcagcgcct cgcggtcggg gccggtccgg ggattctggg
      241 cgttgccgat caggttggag agaatccgca tcagaatctg ccgcggcgtg accgcgtcaa
      301 aatgttcggc cagcggcggc tcgcccgtga gcgcggcgat cttcttctcc gcctcctcgc
      361 gagtcagccg cagaccgcct tcgaagacat cgatgagctg cggcgggcct tccgcgggca
      421 cgtgccgcac gacgaagtgc gccggcaggc cgacgccctc gaacttcagg cccagccgcg
      481 ctccgagctc gatatacaga accgacagcg tgatcggcag cccctcgcgg tcgtcgatca
      541 cacggctcaa gaaactgttg gcccgatggt aatagtccgt gcgactgccg tgaaaaccgt
      601 tgtccttgaa caagtactcg ttcagcgccg cgagcttgtc ggcctcggtc gccttggcgg
      661 gcagcttgtg cttgatctcg gccgccatgc ggtccacata ggccgcgtaa gccgcgacgt
      721 cgaggtcttc gtcgccgagc cgggcgatcg tgagcgcggc ccgcagcaga tcgatttgct
      781 gcacctccgc gccggccact ttggtaaact ccgcggcgat ggcagccgtt cgcacgtcgg
      841 ccgccaggcg cttcaggtcg gcggcccgtg cttccagctc cgcggccttg gccagcagcg
      901 cggcctgggc cggttcgctg tcggcgctgg ccagatcggc gagcgccgcc tcgcgggcct
      961 cggccagcgg tggcagtttg tcgatcgcgg ccgcgagctt cgaccgcacg tcgtccgctg
     1021 ggcgctcgcc gggcagttcc ttgccgacgg cgaagcggcg aaactccgcg ccggtgttgc
     1081 ggaacttggc cagtccgatg cggcccttcg cgaaggcgtc ctccgcggcc tcgatcacca
     1141 gctcgtcgtt cacgaagcag cggagcttac ctttttcgac gcggaccttc agccggttcc
     1201 actcgccggg gcggtagtgc tccgacggtt tctcggcgag gacttgccac gaaaagacgt
     1261 ccgggccttc gaagcggctg agccgcagct tgccgttcga cgggtagaag ccgtaatgct
     1321 tgtcgccgcc gtcggcgtga aacacgaggc ccgcggcgcc gctttcgtcg tcgagcttaa
     1381 cctgcacggc cagctcgtaa ggcacggccg gcgaatcgcc cttggacaag agcagcgacc
     1441 ggccgccgaa tccggccccg acgccatcca ccagcagccg gccggcacgc tgctgccaac
     1501 gcgcgccgaa cagcggctcc cactgcgtgc gatcgatgcc gccgatggtc agccagcggt
     1561 cgatgggcac cggattgggc ttatcgcgca gcgtcttgag gtcggccgcg gcgaccgcga
     1621 agcccagatt ctgggtgacg agcgacttca tcgtgacgat gcccagcacg cggccctgca
     1681 tgtcgagcac cggcccgccg ctgttgcccg gctcgaccgg aatcgccagc tgcagcatgc
     1741 tgcggccgtc gatctcgcgc gtgccgctga cgacgccact gaccacgctg tgcttgagcc
     1801 cctgcgggtt gcccatgacg acaacgggct cgccggcgtc gatcttttcc gcgccgctca
     1861 actccagcgg tcgcaggtcg gtcgcatcga cctcgacgat ggccaggtcg agcgcccggt
     1921 cggaagcgtg cacggccttc accgtcagcg tctttccgcc ggccgtctgc accgaaatcg
     1981 gccgcgcttc gccaatgacg tgcaggttgg tggcaatcag cccgcctttg tcgatgacga
     2041 atccggtgcc gagtccctgc tgcgctccct cgcggccggc gaagccgatg acgacgatcg
     2101 agtcccggac tttcgcggcg agatccttga cggagagcgg ctcgtcggcg acgcccaccg
     2161 ctggcaacag gagcgtgagc gcgaaggtaa ttcggtgcat ggcgatgttc ctgcgggcca
     2221 actacttgcc ggccgcggtg agtttctcca gagcccgcgt cgaaatgcgt ttcacgtatt
     2281 cgctggaatc ggcccgcgcg gcacggagcg tcgcttcggc gggacgagcc gccggcccga
     2341 gtttctccag cgcggcgact gcttgaatgc gcgtcgcctc ctgcggatcg ttcatcgcgg
     2401 cgaccagcac ccgcagcgcc ctgtcgcttt ggcccaactg ccccagggcg gacgccgcgg
     2461 caattcgcgg agcgggcgat ttgtcgtcca gcagcggcac cagcgcgccg attgcgccgg
     2521 cgcggccgcc gtcgccctgg ggcagatgct ctgcaaatgc tgccagtccc agcgcgcccc
     2581 aataccggac cgacggatcg ccgtcgctga tcagcttgac cagttcggcg aactgcacgt
     2641 ggtcgctggc gtcaccgctg gcacggccgg ccatctgcgc caaggcccag gccgcgtgga
     2701 ctcgctgctg gccggtcgtg gtcgtcaggg cctggctcca ctgctcggcc gtgcggccca
     2761 ggaacttctc ctgggcgatc acagcccagg ggagcaacac gatcgcagcg gcaatgacag
     2821 cccgaaccat gcgtcgctac tccttcggct tgaggttgat cttccgtttc cacgtcgcct
     2881 tgcgctgctt cgccttcggg tgatgccgcg gagcaatgtc ctgcgtcggc cgcggcagca
     2941 cgccgcccgg catccgctgg agcagttcca ccttggccgc ccgcggcgct tgcttcgtcg
     3001 tcagcttgcg gcgagcctcc cgctctttct ccgtcagctt ctggatctcg aacctcacag
     3061 gctgccccgg ctgcaggccg tcgaagacct ggctgccgac ggtcgtcgcg tggaaataga
     3121 cgtccgggcc gtcgctgcac ggctcgatga agccgaagcc gcggtcgtgc gacaggttct
     3181 tgatatagcc gtaaagcatg tgactcagtc tgacccaaat cgcgccgctg agctagacag
     3241 cggcccgcgt gacggcgacg ccgcggcgcg cgagggccgc ggcgatgcgg gccatctgct
     3301 ggcgcgtcag ccgccagccc atcgcgccgg cggtttcgcg aatttgctgc gggcgcttcg
     3361 cgccgcagag ggccgcggta atccccggct gctggatcgt ccagttgacg acgacctgcg
     3421 cgacggtctt gccaatctcc tccgcgaccg ccgagagctc tcccaggaaa tcctggttct
     3481 tctgccactc ctggccctgg aacatcggat acttggcccg accgtcgccc ggctgaaaca
     3541 cgtggtcccg cggcagccgg ccggccagga ggcccttcat cagcggccag tagacgcaga
     3601 ctgcgatgtt ccgttcgcgg caccagggga gcgtgtcccg ctcgatgtcg cgctgcagca
     3661 tgttgtacgg cggctgaaag acctcgatgg ggcaagcggc gtgaaactcc tcgagctgcg
     3721 gcagcgtgaa gttcgaaacg ccgaccgcca gagtctggcc ctcccgcttc aggcggagca
     3781 actcggcggc cgaatcgacc agagtcgtct ctggatcagg cgcatgcaga tacagcagct
     3841 cgacccggtc cgtattgagg cgacggaggc tttcttcgca ctggcggcgg agcgtggccg
     3901 gccggccgtc gatgacccgc tcgcggtgcg cgttccactc gatgccgcat ttcgtggcaa
     3961 tgaccacgtc gccgcggcgg ccggcaatcg cgcgggtgat gagccgctcg ctttccccgc
     4021 cagcgccgta agcataggcg gtgtcgaaga agttgacgcc cgcatccagg gccgcgtgca
     4081 gcgtcgccag gctgtccacc tcgttcacgc ccaagctggt catgcccgaa atcggccagc
     4141 agccgagggc gaccggcgaa acttggagac ccgtattgcc gagcgggcgc agctcg
//
LOCUS       DASRJB010000266         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3743448, whole genome shotgun sequence.
ACCESSION   DASRJB010000266 DASRJB010000000
VERSION     DASRJB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743448"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFB80_07150"
     CDS             complement(<1..579)
                     /locus_tag="VFB80_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN33577.1"
                     /translation="MKRILFFAYGVLCHLLFLVIYAWLACFLGNLLVPSTIDGPAAGG
                     STAAAIVVDLLLLAAFALQHSVMARPAFKRLWTRLVPEPIERSTYVLISCLVTALLIW
                     QWRPIPLVVWSVQQPVGWWILSALFAVGVLAVPGVSLMINHFDLFGTRQVWLYLRGKE
                     YTPLPFRTPLAYSWVRHPLYVAWALSFWATPTM"
     gene            841..1422
                     /locus_tag="VFB80_07155"
     CDS             841..1422
                     /locus_tag="VFB80_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN33578.1"
                     /translation="MPTGTRQRLIEAATKRFYRDGFRNVGLDAILADVGISKTAFYKH
                     FESKDDLILAALADRSRWIQDTFRQLMRERGGELPAGQLRALMDVVATITDSDGYQGC
                     IFVNVSLEFPLPHEPAHLAASQSKADLESIVCAVAAEAGAADPRALAQELCLIMEGAY
                     VTRHVTGNRQTIDIARRLAERAIGAHLATERPA"
     gene            complement(1437..3551)
                     /locus_tag="VFB80_07160"
     CDS             complement(1437..3551)
                     /locus_tag="VFB80_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN33579.1"
                     /translation="MTHSLPTIAILLLSAWLAYAAAGQSPKFEWQTATPASQGMSAEK
                     LDELQESLALKNTKALLVIRNERIVWEWYAEGHGPTKTHYTASMAKALVGGVALGVAI
                     TDGRIALDDPAAKYIPEWKSDPLKSKITVRQLGSHSSGVEDAEVDNIIPHDKLLGWKG
                     DFWKRLPVPNDPFSVSRDKAPVSFEPGTQGAYSNPGIALLTYCTTAAIAGGEHKDVRT
                     LLRERVMRPIGAADNEWSVGYGQTSNVNGLPLVGSWGGGNFTARAAARVGRLMLREGN
                     WEGKQLLSKQAVRDITSDAGLPGPWGMGWWSNNSGHHAKLPKDAFWAAGAGHQNLLVV
                     PSLNLIAVRNGGNLGPDDERRSAVNTFLFEPLLAAIVSEQTKQSAAPYPPSQTIAGIE
                     WAPKESIVRKAKGGDNWPLTWADDDHQYTAYGDGWGFEPLIKEKLSLGLCRVEGTPPM
                     FSGVNLRSESVEQKGEGKNGKKASGLLMVDGVLYMLVRNAGNSQLAWSKDRGQTWTWS
                     NWRFTTSFGCPTFLNFGRNYAGARDDYVYVYSLDGDSAYEPVDQWVLARVPKNRITDQ
                     SAYEFFQKPDASGGATWTKQIDQRGAVFRHPGNCYRSGITYNAGLKRYLWCQILPTST
                     HPQGPRFQGGFGVYDAPEPWGPWTTAFYTTEWDVGPGETSSFPAKWMSSDGQTLHLVF
                     SGDDYFSVRQAKLKLAGQASGR"
BASE COUNT          631 a         1198 c         1197 g          639 t
ORIGIN      
        1 catcgtcggc gtcgcccaga acgacagcgc ccaggcaacg tagagcggat gccgcaccca
       61 ggagtaggcc agcggcgtgc ggaacggcag cggggtgtat tccttcccgc gcagatacag
      121 ccaaacttgc cgcgtgccga acaggtcgaa gtgattgatc atcaggctga cgccaggcac
      181 tgccaggacg ccgacggcaa acagggccga aagaatccac cagcccaccg gctgctgcac
      241 gctccagacc accagcggaa tcggccgcca ttgccagatg agcagggcgg tcaccaggca
      301 cgaaatcagc acgtaggtgc tgcgttcgat cggttcgggc acgagccggg tccagagccg
      361 cttgaaggcc ggccgcgcca tcaccgaatg ctgaagggca aaggccgcca gcaggagcag
      421 atcgacgacg atggccgcgg ctgtggaccc gccagcggcg ggaccatcga tggtgctagg
      481 taccagcagg ttgccaagga agcaggccag ccaggcatag atgacgagga acaggaggtg
      541 acacaagacg ccgtaggcga agaacaggat tcttttcatg gggtctccgt gagtaggccc
      601 cttctgccgg aaggggcctt gaggaagcgc agcgaccagg gacattgccg aggattcgag
      661 cttgccgctg cccaggtccc gcccggcaga cgggacctac acatccgcaa aatgatgcgc
      721 acgggcaacg cggccgactc gataattcgc gaattcaagc gattggaaag ttgacttgcg
      781 gtacaccgcc cggttgacta ccggtacacc ttgctcgaga ttccaggccg gaggactcgc
      841 ttgcctacag gcacccgcca gcggctgatc gaagccgcga ccaaacgctt ctatcgcgac
      901 ggcttccgca acgtcgggct ggatgccatc ctggccgacg tcggcatcag caagacggcg
      961 ttctacaagc acttcgagag caaggacgac ctgatcctgg cggccctggc ggaccgcagc
     1021 cgctggattc aggacacgtt tcgccagctg atgcgcgagc gcggcggcga gcttcccgcg
     1081 gggcagctgc gagcgctgat ggacgtcgtc gcaacgatca cggattccga cggctaccag
     1141 ggctgcatct tcgtgaatgt gtcgctggag ttcccgctgc cgcacgagcc ggcgcatctg
     1201 gccgcgagcc agagcaaagc ggacctcgaa agcatcgtct gcgcggtggc cgccgaggcg
     1261 ggggcggccg atccgcgggc cttggcccag gaactgtgcc tgatcatgga gggggcctac
     1321 gtgacgcggc acgtcaccgg caaccgccag acgatcgaca ttgcccggcg gctggcggag
     1381 cgggcgatcg gcgcgcacct ggcaaccgag cgcccggcgt agctgcccaa gactggtcag
     1441 cgcccgctcg cctgaccggc gagcttcaac ttcgcttgcc gcacggaaaa atagtcgtcg
     1501 ccggaaaaga ccaggtgcag cgtctggcca tccgaactca tccacttcgc cggaaagctc
     1561 gaggtttcgc cggggccgac gtcccactcg gtggtgtaga acgccgtcgt ccacggcccc
     1621 cacggctccg gcgcgtcata gacgccgaag ccgccctgaa agcgcgggcc ctgcgggtgc
     1681 gtgctggtcg gcaaaatctg gcaccaaagg tagcgcttca ggccggcgtt gtaggtgatg
     1741 cccgagcggt agcagttgcc cggatggcgg aagaccgcgc cgcgctggtc gatctgcttt
     1801 gtccacgtcg cgccgcccga ggcgtcgggc ttctgaaaga actcgtaggc ggactggtcg
     1861 gtgatgcggt tcttcggcac gcgggccagc acccactggt ccaccggctc gtacgcgctg
     1921 tcgccgtcga gcgaatagac atacacgtag tcgtcgcgag cccccgcata gttccgccca
     1981 aagttcaaaa acgtcgggca gccgaagctg gtcgtgaatc gccaattgct ccaggtccag
     2041 gtctggccgc ggtccttcga ccaggcgagc tgcgagttgc ccgcgttgcg gacgagcatg
     2101 tatagcacgc catcgaccat cagcaggccg ctggccttct tgccgttttt gccttcgccc
     2161 ttttgttcga ccgattccga gcggaggttg acgccgctga acattggcgg cgtgccttcg
     2221 acccggcaca ggccgaggct gagcttctcc ttgatcagcg gctcgaatcc ccagccgtcg
     2281 ccgtaggccg tgtattgatg gtcgtcgtcg gcccaggtca gcggccagtt gtcgcccccc
     2341 ttggctttgc ggacgatcga ttccttgggc gcccactcga tgccggcgat tgtctggctc
     2401 ggcggatagg gtgcggctga ttgcttggtc tgctccgaaa caatggcagc cagcagcggc
     2461 tcgaacagaa acgtattgac ggcgctgcgc cgctcgtcgt cgggcccgag gttgccgccg
     2521 ttgcggacgg cgatcaggtt caggctgggc acgacgagca agttctggtg gccggccccg
     2581 gcggcccaga aggcgtcctt gggcagcttg gcgtgatgcc cactgttgtt gctccaccag
     2641 cccatgcccc acgggccggg cagtccggcg tccgacgtga tgtcgcggac ggcctgcttg
     2701 ctcagcagct gctttccctc ccaatttccc tcgcggagca tcagccggcc gacgcgggcc
     2761 gcggcgcgag ccgtgaaatt gccgccgccc cacgagccga ccagcggcaa gccgttcacg
     2821 ttgctcgttt gcccatagcc gactgaccat tcattgtcgg ccgcgccgat cggccgcatc
     2881 acgcgctcgc ggagcagcgt gcgaacgtcc ttgtgctcgc cgccggcgat ggccgcggtc
     2941 gtgcaatacg tgagcagggc gatgccgggg ttgctgtacg ccccttgtgt gcccggctcg
     3001 aacgagaccg gcgctttgtc gcgcgagacg ctgaacggat cgttgggcac tggcagccgc
     3061 ttccagaagt cccctttcca gccgagcagc ttgtcgtgcg ggatgatgtt gtccacctcg
     3121 gcgtcctcga cgccggagct gtgcgagccg agctgccgca cggtgatctt cgacttcagc
     3181 ggatcgctct tccactccgg aatatatttc gccgccgggt cgtcgagcgc gatccgcccg
     3241 tcggtgatgg cgacgccaag cgccacgccg ccgacgagcg ccttggccat cgaggcggtg
     3301 tagtgcgtct tcgtcgggcc gtgcccttcg gcgtaccact cccagacgat ccgctcgttg
     3361 cggatcacca gcagcgcctt ggtgttcttc agcgccaggc tctcttgcaa ctcatccagc
     3421 ttctcggccg acattccctg gctggccggc gtggcggtct gccattcgaa tttcggactt
     3481 tggccggcgg ctgcgtatgc cagccaggcg gacaaaagca ggatcgcgat cgttggcagg
     3541 ctgtgggtca caacgcacct cgaggcggct ttagctgacc cctgaaaagg tggtcgcgcg
     3601 ctgccttgtt tcgctttttc agtctgtatc ttctttctgg ccatggactt acgtcaaaca
     3661 ctcga
//
LOCUS       DASRJB010000267         5555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1487776, whole genome shotgun sequence.
ACCESSION   DASRJB010000267 DASRJB010000000
VERSION     DASRJB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487776"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1244
                     /locus_tag="VFB80_07165"
     CDS             96..1244
                     /locus_tag="VFB80_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN33580.1"
                     /translation="MPDREEPVPPQPEFAIARVTDAPAPAATGDERPPPKGAPDVRQG
                     LTGRKEGKKADLLKEYGGTAGTEQAVEAGLAWLAKQQDEDGGWRLQGSFSNAGQLESR
                     TAATAMALLAFQGAGHTHREGKYSALVERGWKFLLGRQQQDGSFRGEAGTLYAHAQAT
                     IALCEIYGMTHDEQFEEPAKQAVEYCLAAQADDGGWRYGPKMPGDVSVTGWFVMALQS
                     ARMAELPVPGDALTRVGHFLDACSPTDGVTYSYMPGGGATPAMTAEALLCRQYLGWPR
                     DDPRLMAGVAQLLRNPIHPKAHNAYYWYYATQVCHHMEGEAWKAWNRALRQELPKLQE
                     KSGPEKGSWPPGSDHHAQTAGRLYETCLALYMLEVYYRHLPIYRYRPR"
     gene            1255..2568
                     /locus_tag="VFB80_07170"
     CDS             1255..2568
                     /locus_tag="VFB80_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HZN33581.1"
                     /translation="MSQWYCKLGGAAAGPFSRDELDYLFHRGQLTSDTQMRLADERNW
                     SSAADLLTDLFPPPAAPAQPARQPVSQPAAEPAVPAPAKPKSQPAASKDESDAQRRRK
                     QLLLGAGIGIGSALLIFLLLLLLLTFGGFGRGNGFAGQGGQGTGVGTGTGSGVGPGSG
                     SGTGGGNGSGTGRGTGGGTQATVSGPRTDAKPKGGGAGGGQAPSTGAGEKTGPGRAPA
                     SDLAAAPPPENSIQKFTEQPPAPAAGGSAAGSGDGSFLGGGGGGGSDGTEFLGLKARG
                     NKIVYIIDCSGSMAGQKLANAKQELIDSIKRLRPQQSAYVFFFASGSYPMFGPDGPVE
                     TKLLKCTPANVQKMEQWINNFNEGGGTNPRESLLAAVDLRPDVIFFLTDGGFHPGVAD
                     EVRTKNHGGEAKVASINTICFTDRSGEPVMQKIANENKGDYKFVP"
     gene            2653..3489
                     /locus_tag="VFB80_07175"
     CDS             2653..3489
                     /locus_tag="VFB80_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33582.1"
                     /translation="MSRRSHASNPISLLWFQDIITSVLGIMILVTLFLALEMIFNRQH
                     SPDIQTREITQKLKEAAAQSAEVQAAVEANRRQIDALRRQLAGQETELLQQVRYDSDQ
                     LQRQLRDLEALNQLLAAELSDADSRRRAAQASRDELTQKDASRDADRQTLEQLSREVQ
                     EKLAELKKLRTANRVIFNLSQGDAKTPWLVELSAAGVQAAPVGKRARPETFPDIRAFL
                     AWARQRSRAAEHFVLLVKPATIRDFIQARSALKAAGFDVGFDLLASDQTALDPEHGAA
                     TP"
     gene            3486..4391
                     /locus_tag="VFB80_07180"
     CDS             3486..4391
                     /locus_tag="VFB80_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33583.1"
                     /translation="MKRRKTLETDNLDLLLDTICDTFGTVLFIAMLVVVLLNQSSPQA
                     ALIAPDAAARQAMASAQTELAATQQDLDTLRRAAADQAELSRLFRDPQLKSLLSQNTS
                     QQTTLTAVAAARAENLQDAAAAQTEANRVAAALQALDDALKDTRQQLAIADQQLAAER
                     QLRTRTAALPKLRSTSKRQIAFLLSGGRLHGYLQIDGGRSVRAAAETREVTDGKATYL
                     EPVPGGGTPVDPAGDKSGQLGQKLAGWNPQQHFISITVWPDSFVHFPALRQLLVERGF
                     EYELMPLADGDRLRVSDAPVSSLVQ"
     gene            complement(4396..>5555)
                     /locus_tag="VFB80_07185"
     CDS             complement(4396..>5555)
                     /locus_tag="VFB80_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33584.1"
                     /translation="APQAAYYAPQAAHYAPSQSYAISPAGGSSAGSEAAAYYGQPTAL
                     NYVPPRYAYRPTYAAVPVYMYRPVTAYDPVMAQPTTCMQPTVASTCQPQRTHFLSWLH
                     PHNWFKGHSWFGGGGGCGSRGCGSPPPVTAYCQTNYCQQPYYPAPQPVVIPTVPAVTT
                     PPTLAPGSTIIAPPAGPRIPSPPSRFLPGPGGGGAADFQPRIVPGTSTPGSTVPLGPI
                     PGSTTPLPGSTFPGSTIPGTTLPGTTLPGTTFPPTTPPGSFGSGTNYPPVVDPYRPSS
                     ATSNPKSSGPVIRAPELNPPLPPSVQPVPDLDARQPVAPLNGAPQLLSPRDKTAAKGD
                     SRWAVVPAVWPSQPAPQAALQRPVVPTKATEPVSASASRYDDRGWTSGRAW"
BASE COUNT          955 a         1893 c         1919 g          788 t
ORIGIN      
        1 cgggacttcc cagccaccat acgcctgcga gccccggcag ctcaacggct ccgcccaggg
       61 cgccgcccac aactgaatcc gcgccggcga cgcccgtgcc ggatcgcgaa gagccggtgc
      121 cgccgcaacc ggaatttgcg attgcccgcg tgaccgatgc tcccgcccca gccgcgaccg
      181 gcgacgaacg cccaccgccg aagggtgcgc cggacgtccg ccagggcctg accggccgca
      241 aggaaggcaa gaaggctgac ctgctcaagg aatacggtgg aacagccggc accgagcagg
      301 cggtcgaagc cggcctggcc tggctggcga agcagcaaga cgaggacggc ggctggcggc
      361 tgcaaggttc gtttagcaat gccggccagc tcgagagccg gaccgccgcc acagcgatgg
      421 ctctgctcgc gtttcaaggc gcgggccaca cgcaccgcga aggcaagtac tccgcattgg
      481 tcgagcgggg ctggaaattc cttctgggcc gccagcagca ggatggctcg tttcgcggtg
      541 aagccggcac gctctatgcc cacgcccagg ccacgatcgc cctgtgcgaa atctatggca
      601 tgacgcacga cgagcagttc gaagagccag ccaagcaggc cgtggaatac tgcctcgccg
      661 cccaggctga cgacggcggc tggcggtatg gacccaagat gcccggcgac gtgtcggtca
      721 ccggctggtt tgtgatggcc ctgcaaagcg cccgcatggc cgagctgccg gtgccaggcg
      781 atgcgctgac gcgggtgggc catttcctcg acgcctgctc gccgaccgac ggcgtgacct
      841 acagctacat gccgggcggc ggcgctacgc cggctatgac cgccgaagcc ctcctttgcc
      901 ggcagtatct gggctggccg cgggacgatc cgcggttaat ggccggagtc gcgcagctgc
      961 tgcgaaatcc aatccacccg aaggcccaca acgcgtatta ttggtactac gccacgcagg
     1021 tctgccatca catggaaggg gaggcctgga aggcgtggaa cagggcgctg cgacaggagc
     1081 tgcccaagct gcaggagaag agcgggcccg aaaaggggag ctggccgccc gggagcgatc
     1141 accacgctca gactgctggc cggctctatg agacctgttt ggcgctgtac atgctggaag
     1201 tctattaccg tcacttgccg atctatcgct atcgacctcg ttaggtgcgc gcggatgtcg
     1261 cagtggtatt gcaaactcgg cggcgcggcg gccggcccgt tctcgcggga cgagctggac
     1321 tacctgtttc accgcggcca gttgacgagc gatacgcaga tgcggctcgc cgacgagcga
     1381 aactggagta gcgcggcgga tctgttgacc gacctctttc cgccgcccgc cgcgccggcc
     1441 cagcctgcca ggcagccggt ttcgcagccc gcggcggaac cagccgtccc cgcgccggcc
     1501 aagccaaagt ctcagccagc ggccagcaag gacgagtccg acgcccagcg gcggcgcaag
     1561 cagttgctcc tgggcgcggg catcggcatc ggctccgcgc tgctcatttt tctgctgctg
     1621 ctattgctgc tcacgttcgg cggatttggc cgcggcaatg ggttcgccgg ccagggcggg
     1681 caaggcacgg gcgtcggcac gggcacgggt tccggcgtcg gtcctggcag tggcagcgga
     1741 acgggcggcg gtaacggctc ggggaccggc cgcggcacgg gcggcggaac tcaggcgacc
     1801 gtaagcggtc cccgcacgga cgcgaaaccc aaggggggcg gtgctggcgg cggccaggct
     1861 cccagcaccg gcgcgggcga gaaaaccggc ccgggacgag cgccggccag cgacctggcg
     1921 gcagctccgc cgcccgaaaa ttcaatccag aaattcaccg agcagcctcc cgcgccggcc
     1981 gcgggcggct cggcggccgg cagcggcgac ggctcgttcc tgggcggcgg cggaggcggc
     2041 ggctccgacg gaaccgagtt tctcggactc aaggcccgcg gcaataagat tgtctacatc
     2101 atcgactgct cgggcagcat ggccggccag aagctggcca atgccaagca ggagttgatc
     2161 gactcgatca agcggctgcg accgcaacag agcgcgtacg tcttcttctt cgccagcggc
     2221 tcgtatccga tgttcggccc ggacggtccg gtggaaacga agctgctgaa atgtactccg
     2281 gccaacgtgc agaagatgga gcagtggatc aacaacttca acgagggcgg cggcacgaat
     2341 ccccgcgaat cgctgttggc cgccgttgac ctgcggcccg acgtgatctt cttcctcacc
     2401 gatggcggct tccatcccgg cgtggccgac gaagtgcgaa ccaagaacca cggcggcgag
     2461 gccaaggtcg ccagcatcaa cacgatctgc ttcaccgacc gcagtggcga gccggtgatg
     2521 cagaagatcg ccaatgagaa taagggggat tacaagtttg tgccgtgatt tgtcagtggt
     2581 cagtcgtcag tggtcagttg agccgccacc gacatccaac ggactactga ccactgacaa
     2641 ctgacaattc caatgtcgcg acgcagccac gccagcaatc cgatctcact gctctggttc
     2701 caggacatca tcacttcggt cctggggatc atgatcctgg tgactctgtt tctggcgctg
     2761 gagatgatct ttaaccggca gcattcgccg gacatccaga cccgcgagat cacgcaaaag
     2821 ctgaaagagg ccgcggccca gtcggccgaa gttcaggcag ccgtagaggc caaccgccgc
     2881 cagatcgacg cgctgcggcg gcagctcgcc gggcaggaga cggagctgct gcagcaagtc
     2941 cgctacgact cggaccagct ccagcggcaa ctccgcgacc tggaggcgct taaccagctg
     3001 ctggccgcgg agctgtccga cgccgattcc cgccgccgcg ccgcgcaggc ctcgcgcgac
     3061 gagctgaccc agaaagacgc cagccgcgac gccgaccgcc agacactgga gcagctgtcg
     3121 cgcgaagtgc aggagaagct cgccgagctg aagaagctcc gcacggccaa ccgcgtcatc
     3181 ttcaacctca gccaggggga cgccaaaacg ccctggctgg tcgagctctc ggccgccggc
     3241 gtgcaggccg ccccggtcgg caagcgggcc cggcccgaaa cctttcccga catccgggcg
     3301 tttctggcct gggcccgcca gcgcagccga gcagccgagc acttcgtgct gctggtcaag
     3361 ccggccacga tccgcgactt catccaggcc cgctccgcgc taaaggccgc gggcttcgac
     3421 gtgggcttcg acctgctcgc cagcgaccag acggccctgg accccgagca tggagccgcc
     3481 accccatgaa acgccgcaag acgctggaga ccgacaacct cgacctgctg ctggacacga
     3541 tctgcgacac gttcggcacc gtcctgttca tcgcgatgct ggtggtcgtg ctgctcaacc
     3601 agtcgagccc gcaggcggcc ctgatcgcgc cggacgccgc ggcgcggcag gcgatggctt
     3661 ccgcccagac cgagctggcc gccacgcagc aggacttgga cacgctccgc cgcgccgccg
     3721 ccgatcaggc ggagctatcg cggctgtttc gcgatccaca gctcaagtcg ctgctctcgc
     3781 aaaacacgtc gcagcagacg acgctgaccg ccgtcgccgc cgcccgggcc gagaacttgc
     3841 aggacgccgc cgccgcccag accgaggcca accgcgtcgc tgctgctttg caggccctcg
     3901 acgacgccct caaggacacg cggcagcagc tggccatcgc cgatcagcag ctcgcggccg
     3961 agcggcagct ccgcacccgc acggccgccc tgcccaagct gcgcagcacc agcaagcggc
     4021 agatcgcctt cctgctcagc ggcggccggc tgcatggtta cctgcaaatc gacggcggcc
     4081 ggtccgtgcg ggccgcggcc gagacccgcg aagtgacgga cggcaaggcg acgtacctgg
     4141 agcccgttcc cggcggcggc acccccgtcg atccggccgg cgacaaatcc ggccagctgg
     4201 gtcagaagct cgccggctgg aatccccagc agcacttcat ctcgatcacc gtctggcccg
     4261 actcgttcgt gcacttcccg gcgctgcggc agctgctcgt cgaacggggc ttcgagtacg
     4321 agctgatgcc cctggccgac ggcgatcggc tgcgggtgag cgatgccccg gtaagcagcc
     4381 tcgtgcagta gcgatctacc acgcgcggcc gctggtccaa cccctgtcgt cgtagcgcga
     4441 ggcgctggcg gagaccggct cggtggcctt cgtcggcacg accggccgct ggagcgctgc
     4501 ctgcggcgcg ggctggcttg gccagaccgc gggcacgacc gcccaccgcg aatcgccctt
     4561 ggccgccgtc ttgtcgcgcg gcgaaagcag ctgcggcgcg ccattcagcg gcgcgactgg
     4621 ctgccgcgcg tcgaggtccg gcaccggctg cacgctcggc ggcagcggcg gattgagttc
     4681 cggagcgcgg atgaccggtc cgctgctctt cggattgctg gtggcgctcg agggacgata
     4741 cggatcgacg accggcggat agttcgtgcc cgagccaaag ctgcccggcg gggtcgtggg
     4801 tgggaacgtg gtgcccggta acgtcgtgcc gggcagtgtg gtgccgggga ttgtggagcc
     4861 agggaatgtc gaaccaggca gcggcgtcgt gctgccggga atcggcccca aaggcaccgt
     4921 cgaaccaggc gtggatgtgc cggggacaat ccgcggctga aaatcggcag cgccgccgcc
     4981 gccgggcccc ggaagaaatc gcgagggcgg cgaagggatt cgcgggcccg cggggggcgc
     5041 gataatcgtg ctgcccggtg cgagcgtcgg cggcgtggtg accgccggca ccgtggggat
     5101 tacgactggc tgcggcgcgg gatagtaggg ctgctggcag taattcgtct ggcaatacgc
     5161 ggtgactggc ggcggactcc cgcagccgcg ggatccgcag ccaccgccgc cgccgaacca
     5221 ggagtggccc ttaaaccagt tgtgcggatg cagccacgag agaaagtgcg tccgctgcgg
     5281 ctggcaggtg ctggccacag tcggctgcat gcaggtcgtg ggctgagcca tcaccggatc
     5341 gtaggccgtc accggccggt acatgtaaac gggcaccgcg gcgtaggtcg ggcggtaggc
     5401 atagcgcggc ggcacgtagt tgagagccgt cggctggccg tagtaggccg cggcctcgct
     5461 gcccgccgag ctgccgccgg cggggctgat cgcgtacgac tggctgggag cgtaatgcgc
     5521 ggcctgcggg gcatagtacg cggcttgcgg agcgt
//
LOCUS       DASRJB010000268         7684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2043151, whole genome shotgun sequence.
ACCESSION   DASRJB010000268 DASRJB010000000
VERSION     DASRJB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043151"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..609
                     /locus_tag="VFB80_07190"
     CDS             283..609
                     /locus_tag="VFB80_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33585.1"
                     /translation="MHLTGPACSATDEEEPQEGHTYRFNGNPYLHAGSIHSVEMAELE
                     SRVTQFEAKLADPDDPDDKKWTARWLERFRKELNKKQAGRSLKQREKRDQRRTKRNIQ
                     PERGGM"
     gene            671..940
                     /locus_tag="VFB80_07195"
     CDS             671..940
                     /locus_tag="VFB80_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33586.1"
                     /translation="MSDDEPLYVDCGPHGKRVAAVVCCHMLESSEPVGVIENSSDPND
                     LQAWCEKCEEMFLAEGDKTDAFEAFNDRAIVCCVCYQELKSKHSK"
     gene            1066..1464
                     /locus_tag="VFB80_07200"
     CDS             1066..1464
                     /locus_tag="VFB80_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN33587.1"
                     /translation="MRNLISSGSPYEPKIGISRAVRVGNVVAVSGTAPIGADGRTVGI
                     GDAAAQARRCLEIIAGALQKAGASLRQVVRTRTLLVRIEDWEAVSAVHGEFFGQIRPA
                     NTTMQVSRFIDPDWLVEFEADAIIDDEQNA"
     gene            2216..3142
                     /locus_tag="VFB80_07205"
     CDS             2216..3142
                     /locus_tag="VFB80_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN33588.1"
                     /translation="MTTQLEQPPAARPVPSASDVIIETRNLTKVYRDFWGRQKVRALN
                     ALDLQIRRGEIFGLLGPNGSGKSTTIKLLLGLLFPTGGQALVFGKEATEVTKNERIGY
                     LPEESYLYKFLNAEETLDFYGRLFDMSAEVRKRRTDELIEMVGLNWARRRQLREYSKG
                     MTRRIGLAQALINNPDLILLDEPTSGLDPIGTREMKDLILKLRDQGKTILMSSHLLPD
                     VQDVCDRIAILHQGELKELGRVDELLTVQDVTQVRARGLSKAAEEEIRAVIARHQGEL
                     LAVENPTLSLEELFLNIVRDSEARPGRRAGQN"
     gene            3231..5168
                     /locus_tag="VFB80_07210"
     CDS             3231..5168
                     /locus_tag="VFB80_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN33589.1"
                     /translation="MALERGIPDFWIWLLGNAERFNILNPTTWGFLLGAVVICLLLLV
                     IAPFICFLVASLQYGPSEAFYYVARALFSAVTEDLPRFSPRRTWAIARLAIQEAIRNR
                     VLVGFGVFVLLLLFAGLFLDVNNNNPTRVYLSVVLWSTNLLVILMALFLSAFSIPNDI
                     KNRTIYTVVTKPVRASEIVLGRIVGFATVGTAMLVFMGVVSYFFVSRSLDHGHQVEAD
                     TVKEEYEKAPEGQPAPAQRKLLRISGETTFDRHHRHTFELGPDGKGKTNVVQGHWHEV
                     QRTAEGKYIVGPPQGHLIAKAPHYGKLVIYDRDGKVTKGINVGNEWEYRGYIAGGTPG
                     VQTKAAAVWTFSGITPENYPPDKYPSGLPLELTLRVFRSFKGQIERGVLGEMVIRNPN
                     PEARGLASGPILFESKEFVTDSRTIPWKLNSERGGEAGRGELDLMKDLVHEGQIEVAI
                     RCVDPAQYFGVAEPDVYLRPGDASFEMNFFKSYLSIWLQMLLVTAFGVMFSTFLSGPV
                     ALLASLSAIVLGFFGSDVRAVTLGVLYGGEAQGAQYGGGPIESFIRIIMQNNVMTEME
                     INSILSTIIKVLDGGLMCTLYAATYVLPDYTQFDTAGFVADGYNIFGALVAEQLLMGL
                     VYFTAVTITAYFFLKTREVAA"
     gene            5165..6943
                     /locus_tag="VFB80_07215"
     CDS             5165..6943
                     /locus_tag="VFB80_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33590.1"
                     /translation="MSRNKSFLRKVCYIAAIALLLLPIAALSQPATVDTNLADGRDDS
                     SPGGRLSQLRTQYNLAQARLGEIDPASETMKLASLGLRGVAANILWLQANHYKKIEDW
                     DKLEATVNQIIRLQPNFVEVWDFQAHNLSYNVSVEFDDYRMRYEWVKKGINFLIEGTH
                     YNRDEPGLLSQLGWFTGQKVGRADEQVQFRRLYAVDHDFHDVIRKHGINLDEATTGLT
                     VEDPKTHQRTSAIDNWLTARLWYDKAVDAVTYSGKPIRGRSPLLFYSGAPMSRINGSA
                     AMQKDGRFFEAPRLSWERALAEWTSYGNRELPTSAGFNIRLNDQEAADARITQAQAEL
                     EKICGNVKEKILAQKRESLSAETKAALDKPVEQRTEAEHMLVYNAAGALNVAARDYLD
                     YAPREQRRRVQQVIDQIEEDQLTSMQIGLNRRIVNFEYWRTRCQAEKTEEIQQAHFDV
                     YEADRMKADVAKWDEAKQKYEEAWGLYAKIFEKHPALMINAEAQDLVDSVLKYRDLLG
                     QLDEPFPAKFVLEPLLKEHDKGRQLLDQVKVLQGGGETKPEGNKPADPKPSGDKPADP
                     ASPAAKTAPEPKAGKSADEAAKAKTP"
     gene            6940..>7684
                     /locus_tag="VFB80_07220"
     CDS             6940..>7684
                     /locus_tag="VFB80_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN33591.1"
                     /translation="MRIVSLLSSSTEILFALGLGEQVLAISHECDFPPAALHLPRATR
                     SLVDSSRASLQIDEQVRALAAAGAALYEIDRELICRLAPHLIVTQAQCDVCAVRYQDV
                     LDLARAEPSLARTRVLALNPASLGDILQDIRAVADAAGVAIRGERYVVELQDRIDRVA
                     ARKLQLRPRVVCLEWLGPLMTAGNWTPELIELAGGESCLAEAGRHSGYIEWTQVAACN
                     PDVLLIAPCGFGLARSQAEARRLWQLPGFA"
BASE COUNT         1487 a         2489 c         2420 g         1288 t
ORIGIN      
        1 cgctctactt ccgcatagtc caaggccccg atgggaacga gcgggaagat cccatcgagc
       61 acgacgaata cgagcgagca gtcgaaacgg tccggtcgga gtccatcgac atcacggatc
      121 gggctcggct gatggccgtg agagagcgag gccatgctga ggttgccgaa ctccaaaagc
      181 ggaacctcga gttcaagcaa gtgtgcgaca acttctatcg gtcccgacgg gtgactgaac
      241 cgttcgagaa cctgaacgag tcgtactgga cataacattg cgttgcactt gaccgggccg
      301 gcatgttcgg cgaccgatga agaagaaccg caagaaggcc acacctatcg cttcaacggg
      361 aatccgtacc tccacgccgg tagcatccac tccgtcgaga tggcggagct ggaatcgcgg
      421 gtcacgcagt ttgaggcgaa gctggccgac ccggacgatc cggatgacaa gaagtggacg
      481 gcccgctggc tggagaggtt tcggaaggag ctcaacaaga agcaggcagg gcgcagtctc
      541 aagcagcggg agaagcgaga tcaacgccga accaaacgga acatacaacc tgagcgcggc
      601 ggcatgtagg ctattcaata gttaagctcc ctcagccgcc gcagctggtg agcgttcggc
      661 gggtcagaag atgtccgacg acgaacccct gtatgtcgat tgtgggccac acggcaagcg
      721 ggtggcggcg gtggtctgtt gccacatgct ggagtcgagc gagcccgtcg gggtgatcga
      781 gaacagctcc gatccgaacg acctgcaggc gtggtgcgaa aagtgtgagg agatgttcct
      841 ggcagaaggg gacaaaacag acgcctttga agctttcaat gaccgggcca tcgtgtgctg
      901 tgtttgctac caggagctaa agagcaaaca ctccaagtag acgacgagcc gaaatgagcg
      961 tcgcacttga ctagctaggc atgacagtac agggccgcca tgacggtaca gggccggttc
     1021 gttgcagccg gcccgggcaa gtgaacgcag ttgttgagca ttaccatgcg aaacctcatc
     1081 tccagtggca gcccctacga gccgaagatc gggatttcgc gggcagtgcg agttggtaac
     1141 gtggttgcag tctccggcac ggcgcccatt ggcgcggatg ggcgcacggt ggggatcgga
     1201 gacgccgcgg cgcaggcgcg gcggtgcctg gagatcattg ccggcgcctt gcagaaagcg
     1261 ggcgcatcgc tccggcaggt ggttcgtacg cgcacgctgc tggtgcgcat cgaggattgg
     1321 gaggccgtct ccgcggtcca cggcgagttc tttggccaaa ttcggcctgc gaacacgacc
     1381 atgcaggtgt cgcggtttat cgatcccgat tggctggtcg agttcgaagc ggatgccatc
     1441 attgacgatg aacaaaacgc ctgaccatgc gctgcagcgc tgagcttgtc tcgtccacaa
     1501 gaaaaagaga gggccgcacg ggagcgggat acccgagcgg ccctgtccag gcgggaagga
     1561 aataggcggg aatccaggcg gcgactctac ccaatcgctg ccaggatagg catccacacg
     1621 tggcggatgc ggtatcagct catctgcctc cttgcaactt gctgtgaaat tgcggtccca
     1681 ttcgtgtcgg ctgaacgcca gccgtctgtc gctgaccaga cataaagcaa cgcctgtgcc
     1741 aagagccaca agaggcgaat cattttgttt gtgtccgatg ccggaattga tcgtaagttg
     1801 ttctgccgga atgtcttact gcactggcgt gcggactttg ccgcctccat ccgggtcgtt
     1861 ccacgctcgg gtcagcgctt gtaaatgttg ctcggcgagt tttcccttgg gcgagagaac
     1921 gaagcgtgac gccggcagct ggcgcccgtt catcgctcgt cgcaattacc tatggggaag
     1981 cgacttacgg cgatacttgc gacgctcccg cgactggctt atagtgccaa gcttcgctgc
     2041 cgaggcaaac tttgcgtgcc ccacgcgggc cttgcagaaa ttacaaggcg aattgaaccg
     2101 cgcccggctg cctgccgtaa aacagtcgct tgccgcgcgt gctgaaagtc cttatccagc
     2161 cagtgtttga ctcattcggg cagattgccc tcagtccttc cccatccgtc cacaaatgac
     2221 gacgcaactt gagcagccgc ctgcggccag accggtcccg tcggcgagcg atgtcatcat
     2281 cgagactcgc aatctgacca aggtctatcg cgatttttgg ggccgccaga aggtgcgggc
     2341 tctgaacgcc ctcgacttgc agatccgccg cggcgagatt ttcggcctgc tcggccccaa
     2401 cggctcaggc aaatcgacca cgatcaagct gctgctcggc ctgctctttc cgaccggcgg
     2461 ccaggccctc gtcttcggca aggaggcgac cgaagtcacg aagaacgagc ggatcggcta
     2521 cctgcccgaa gagtcgtatc tctacaagtt cctcaacgcc gaagagacgc tcgacttcta
     2581 cggccggctg ttcgacatgt cggccgaggt ccgcaagcgg cggaccgacg agctgatcga
     2641 gatggtcggc ctgaactggg cccgccgccg gcagctgcgc gaatacagca aaggcatgac
     2701 gcggcggatc ggcctggccc aggcgttgat caacaacccc gatttgatcc tgctcgacga
     2761 acccacctcc ggcctggacc cgatcggcac gcgggagatg aaggacttga tcctgaagct
     2821 ccgcgaccag ggcaagacga tcctgatgag cagccacctg ctgccggacg tgcaggacgt
     2881 atgcgaccgc atcgcgattc tccatcaggg cgaattgaaa gagctgggcc gcgtcgatga
     2941 gctgctcacg gtgcaggacg tgacgcaggt ccgggccagg ggattgagca aagcggccga
     3001 ggaagagatt cgggccgtca tcgcccgcca ccagggggaa ttgctcgcgg tcgaaaaccc
     3061 gacgctctcg ctggaagagc tgttcctgaa catcgtccgc gacagcgaag cccgccccgg
     3121 ccggcgggcg ggacaaaact gaacaaagaa ccgaagaacc aaagaaccga atctgctgat
     3181 cctttcgttt aggttcttcg gttctttggt tcttcgaatt ttgacctccc atggcactcg
     3241 aacgcggcat tcccgacttc tggatctggc tgctgggcaa cgccgagcgg ttcaacatcc
     3301 tgaatccgac gacctggggc tttctgctcg gcgccgtggt gatctgcctg ctcctgctgg
     3361 tcatcgcacc gttcatctgc ttcctggtcg cctcgctgca gtacgggccg agcgaggcgt
     3421 tttactatgt cgcccgggcg ctgttctcgg ccgtcaccga ggacctgccg cggttctcgc
     3481 cgcgccgcac ctgggccatc gcccgcctgg cgatccagga ggcgatccgc aaccgcgtgc
     3541 tggtcggctt cggcgtcttc gtgctgctgc tgctgttcgc gggcctgttt ctcgacgtca
     3601 acaacaacaa cccgacgcgg gtttacctga gcgtcgtgct ctggtccacg aacctgctgg
     3661 tgatcctgat ggccttgttc ctcagcgcct tcagcattcc caacgacatc aagaaccgca
     3721 cgatctacac cgtcgtgacc aagcccgtgc gggccagcga gatcgtcctg gggcggatcg
     3781 tcggcttcgc gacggtgggc acggccatgc tcgtgttcat gggcgtggtc agctacttct
     3841 tcgtcagccg cagcctggac cacggccacc aggtcgaggc cgacaccgtt aaggaggaat
     3901 acgagaaagc gcccgagggc cagcccgcgc cggcccagcg aaagctcctc cgcatcagcg
     3961 gcgagaccac gttcgaccgg caccaccgcc acacgttcga gctcggcccc gacggcaagg
     4021 gcaagaccaa cgtcgtgcag gggcactggc acgaggtcca gcggacggcc gagggcaagt
     4081 acatcgtcgg tccgccgcag ggccacctga tcgccaaggc cccgcactac ggcaagctgg
     4141 tcatctacga tcgcgatggc aaggtcacca agggcatcaa cgtcggcaac gagtgggagt
     4201 atcgcggcta catcgccggc ggcacgcccg gcgtgcagac caaagccgcc gccgtctgga
     4261 ccttcagcgg catcacgccc gaaaactacc cgcccgataa atacccgagc ggcttgccgc
     4321 tggaattgac cctgcgggtt ttccgctcgt tcaagggcca gatcgagcgc ggcgtgctcg
     4381 gcgaaatggt catccgcaat cccaaccccg aggcccgcgg cctggccagc ggcccgatcc
     4441 tcttcgaatc gaaggagttc gtcaccgaca gccgcacgat tccctggaag ctcaactccg
     4501 agcggggcgg cgaagcaggc cgcggcgaac tcgacctgat gaaggacctg gtgcacgagg
     4561 gtcagatcga ggtcgccatt cgctgcgtcg atccggcgca atacttcggc gtggccgagc
     4621 ccgacgtcta cctccgcccc ggcgacgcca gcttcgagat gaacttcttc aagtcctacc
     4681 tcagcatctg gctgcagatg ctgctggtga cggcattcgg cgtgatgttc agcaccttcc
     4741 tgtccgggcc cgtggccctg ctggcttcgc tctcggcgat cgtgctgggc tttttcggca
     4801 gcgacgtccg cgcggtcacg ctgggcgtgc tctacggcgg cgaggcgcag ggcgcgcaat
     4861 acggcggcgg tcccatcgag tcgttcatcc gcatcatcat gcagaacaac gtgatgaccg
     4921 agatggagat caactcgatc ctcagcacga tcatcaaggt gctcgacggc ggcctgatgt
     4981 gcaccctcta cgccgcgacc tacgtcctgc ccgactacac gcagttcgac acggccggct
     5041 tcgtcgccga cggctacaac atcttcggcg cgctggtcgc cgagcagctg ctcatgggcc
     5101 tggtctattt caccgcggtg acgatcacgg cctacttttt cctgaagacc cgcgaggtag
     5161 ccgcatgagc cgcaataaat ccttcctccg caaggtctgc tacatcgcgg cgatcgcgct
     5221 gttgctgttg ccgatcgcgg ccctcagcca gccggcgacg gtcgatacga acctggccga
     5281 cggccgcgac gacagcagcc ccggcggccg cctctcgcag ctccgcaccc agtacaacct
     5341 ggcccaggcc cgcctgggcg agatcgatcc ggccagcgag acgatgaagc tggcctcgct
     5401 cggcctgcgg ggcgtcgccg ccaacatcct ctggctccag gccaaccact acaaaaagat
     5461 cgaggactgg gacaagctgg aagccaccgt caaccagatc atccgcctgc agccgaactt
     5521 cgtcgaagtc tgggacttcc aggcccacaa cctgtcgtac aacgtctcgg tcgagttcga
     5581 cgactaccgc atgcggtacg agtgggtgaa gaagggcatc aacttcctga tcgaaggcac
     5641 gcactacaac cgcgacgagc cgggcctgct cagccagctg ggctggttca ccggccagaa
     5701 ggtcggccgg gccgacgagc aggtgcagtt ccgccgcctg tacgcggtcg atcacgactt
     5761 ccacgacgtg atccgcaagc acggcatcaa cctcgacgaa gccacgaccg gactgaccgt
     5821 cgaggatccc aagacgcacc agcggaccag cgcgatcgac aactggctga cggcccgcct
     5881 gtggtacgac aaggccgtcg atgccgtgac ctacagcggc aagccgatcc gcggccgctc
     5941 gccgctcttg ttctacagcg gcgcgccgat gtcgcggatc aacggctcgg ccgcgatgca
     6001 gaaggacggc cggttcttcg aggccccgcg gctctcctgg gagcgggccc tggccgaatg
     6061 gaccagctac ggcaaccgcg agctgccgac ctccgccggc ttcaacatcc gcctcaacga
     6121 ccaggaggcg gcggacgccc ggatcactca agcgcaggcc gagctggaga agatctgcgg
     6181 caacgtgaag gaaaagatcc tggcccagaa gcgcgagtcg ctctcggccg agaccaaggc
     6241 cgcgctcgac aagcccgtcg agcagcggac cgaggccgag cacatgctcg tctataacgc
     6301 cgctggggcg ctcaacgtcg cggcccggga ctatctcgac tacgcccccc gcgagcagcg
     6361 gcgccgcgtg cagcaggtga tcgatcagat cgaggaagat cagctcacct cgatgcagat
     6421 cggtctcaac cgccgcatcg tcaacttcga atactggcgg actcgctgcc aggccgagaa
     6481 gaccgaggag atccagcagg cccacttcga cgtctacgag gccgaccgga tgaaggccga
     6541 cgtggccaag tgggacgaag ccaagcagaa atacgaggag gcctgggggc tgtacgccaa
     6601 gattttcgaa aagcatccgg ccctgatgat caacgccgag gcccaggacc tggtcgattc
     6661 ggtcctcaag taccgcgacc tcttgggcca gctcgacgag ccgtttcccg cgaagttcgt
     6721 gctggagccg ctactcaagg agcacgacaa gggccggcag ctgctcgacc aggtgaaggt
     6781 gctgcagggc ggcggcgaga ccaagccgga gggcaacaag ccggccgatc cgaaacccag
     6841 cggggataag ccggccgacc cggcgtcgcc cgctgccaag actgcgcccg agccgaaggc
     6901 gggcaagtcc gccgacgagg ctgcgaaggc caagacgccg tgaggatcgt ctcgctcctt
     6961 tccagcagca cggagatcct cttcgcgctc ggtctgggcg agcaggtcct ggccatcagc
     7021 cacgaatgcg actttccgcc ggcggccttg catttgccgc gggccacgcg atcgctcgtc
     7081 gattcgtcgc gggccagcct ccagatcgac gagcaggtcc gcgcgctggc cgctgccggc
     7141 gcggccctct acgagatcga tcgcgagctg atctgccgac tcgcgccgca cctgatcgtg
     7201 acgcaggccc agtgcgacgt ctgtgcggtc cgctatcagg acgtgctgga cctcgcccgc
     7261 gccgagccgt cgctcgcacg cacacgcgtc ctcgctctca accccgcctc gttgggcgac
     7321 atcctccagg acattcgcgc cgtcgctgac gctgccggcg tcgcgattcg tggagagcga
     7381 tatgtggtcg aactgcagga tcggatcgac cgtgtagccg cccgcaagct ccagctgcgg
     7441 ccccgggtcg tctgcctgga gtggcttggg cccctgatga ccgcggggaa ctggacgccc
     7501 gagctgatcg agctggccgg cggcgaaagc tgtctcgcgg aggccggccg gcacagcggc
     7561 tatatcgagt ggacgcaggt cgccgcctgc aatcccgacg tgctgctgat cgcgccttgc
     7621 ggctttggcc tggcccgctc gcaggccgag gcccgccggc tgtggcaact gcccggcttc
     7681 gccg
//
LOCUS       DASRJB010000269         8049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3460015, whole genome shotgun sequence.
ACCESSION   DASRJB010000269 DASRJB010000000
VERSION     DASRJB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3460015"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2490)
                     /locus_tag="VFB80_07225"
     CDS             complement(<1..2490)
                     /locus_tag="VFB80_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN33592.1"
                     /translation="MVPLILGLIASPSAAQPDSIDRDYARELPRIAPTEPQAALATFQ
                     VSAGFGLEQVAAEPLLASPVALSFDENGRMYVAEMRGYSENRDERLSRVRLLVDENGD
                     GKFDKSTVFAEGLGWPTAVFCWAGGVLVADAPDIFYLKDLDGDGKADERRVLYTGLGL
                     SNVQGLINSFQWGLDNRIYAAVSSSGATLVRPDENDSKPLPIRGRDIAIDPRTWEVTP
                     VSGGAQHGLSFNDWGDRFVCSNSDHLQQVMYEDRYLARNPYLAAPSPRRSIAADGPQA
                     DVFRTSPVEPWRIVRTRLRAQKIVPGIVEGGGRPAGYFTGATGATIYRGDAWPAEWRG
                     LAIIGDVGSNLVHRKRLEADGVGFLGRRIDQQSEFVSSRDIWFRPVQFANAPDGTLYI
                     LDMYREVIEHPASLPPLIKKHLDLTSGRDRGRIYRIVPDGFRQRPLPQLGKATTSELV
                     ATLAHANGWNRDSAARLLYERQDKSAVAPLTKLAAESKEPAARLHALYALDGLGALSE
                     AALLPRLADEHPRVREHAVRLAEKLADSSAAIRERLLAMTSDPELRVRYQLAFSLGEL
                     PATAQRTRALVELAQRDAADGYVRVSVLSSLGEGAGGALSLIAADAGFRDSKEGRELL
                     ASLATQIGKQQRPDDVAETLKTLAALAKENSTLLPPLVQRLAARPGSPLAQQIAAATG
                     GKAEALMKLLIAEAAAKAADENAPLKARVAAVEQLRLASFAGQQPLFAGLFAPAVAAD
                     LQSAALATLASYDAADVAELVLSRFAGFSPRLKSQATDVLLSRPAWTLALLGALEQGQ
                     VSAGDLDPVRLKLLAEHRDAAIRVRAARLLSG"
     gene            2665..3984
                     /locus_tag="VFB80_07230"
     CDS             2665..3984
                     /locus_tag="VFB80_07230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN33593.1"
                     /translation="MSERKQPNSQPTFQSLGLSDVMLDALDLAGYEYPTPIQAGLIPL
                     ALQEIDVVGQARTGTGKTASFAIPILEKLQLKSDIHHPQALVMVPTRELAVQVKDEIV
                     KLSAGRRVHCCAVYGGKPIRQQIEKLRKGAQIVVGTPGRVLDHLARGTVDFSRLKFVV
                     LDEADRMLDIGFRPDIERILKRCPAERQTLLLSATVPPPIAKLAERYMRDPQTINFSP
                     KDIAVETIEQFYFTVDPQRKFDLLLRLLTREQPRQAIVFCRTKRGTEKVYLRLSKKLA
                     DVEAIHGDLNQAQRDRVMAAFRSGKVKILVATDVIGRGIDVTSISHIVNYDIPAFCDD
                     YVHRVGRTGRMGREGIAFTFVAPDEGYELTRIEERINRLLKRAEMKDFEFRSRESLAG
                     VLQGGDHHEGDAHDGEGVPVAAVDRPAPPPPPASAMRRPPKKYRRAL"
     gene            4074..4610
                     /locus_tag="VFB80_07235"
     CDS             4074..4610
                     /locus_tag="VFB80_07235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF015020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN33594.1"
                     /translation="MARQLLILDLDETLFYAALSPRGRPPDFELAGYSVYRRPFLQEF
                     LDFAFENFAVAVWSSATEPYVRAAAREVIARSADLQFIWSMERCTRRFNGETREEYWV
                     KDLKKLKRLGFSLDRVLVMDDSPEKHERNYGNLVPVRPFFGDQADTELRDITPFLDRL
                     QRCEDVRKIEKRNWRNWL"
     gene            4726..6735
                     /locus_tag="VFB80_07240"
     CDS             4726..6735
                     /locus_tag="VFB80_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN33595.1"
                     /translation="MTLRILCLSALLVPVLALADGPTDNIPANVRRIPKLGIEVPADR
                     RAKLEAGLKQLEIKIAAVRGGQDAKAKALLPDVEVYFKAVHDALAYQEFFAPAEIDFA
                     DRALATGHERVDQLAAGQAPWAAASGLVVRGYVSKIDSSVQPYGLVVPESYTGKGPGR
                     FRCDLWFHGRGETLSELAFIRDRTNNVGAIAPDDTIVVHPYGRYSNAFKFAGEIDVLE
                     ALDSVKGRYRVDEDRIAARGFSMGGAAAWHFAVHYPDRWFAANPGAGFSETPEFLRTF
                     QQEELKPAWWEKKLWQWYDCPEWCRNLRHCPTVAYSGEIDRQKQAADVMEKALAGEEI
                     ELTHIIGPQTAHAIHPQSKAEIERRLASLAKIGRDTRPLDVDFVTSTLRYNRLHWVTI
                     DGLAEHWSRASVSASIASTQELEFEAAGVIDITFSFAAGDSPFDLGTKVVIISGEQEL
                     EAPGPASDRSWNVSLHRSAGEWRLGKRPADGLRKIHGLSGPIDDAFMDSFLFVRPTGK
                     AANDAAGKWAAAELERASEHWRRHFRGRARVKDDTALTPEDIAGANLVLWGDPGSNAV
                     LKQIAGKLPIRWGEKQIEAGGQKFAADTHALVAIYPNPLNPSRYVVLNSSFTFRDYAY
                     LNNARQVPMLPDWAVVDLTTPPNSVWPGKIAAADFFDERWQLKNQ"
     gene            complement(6722..7291)
                     /locus_tag="VFB80_07245"
     CDS             complement(6722..7291)
                     /locus_tag="VFB80_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33596.1"
                     /translation="MDSERTAYGPAIAVLLEDRLPELGPGTPNEGARPKLTALALNRS
                     LAGAKIVDFVASRCVLSGLWLWHDFLDESHCLSQDIDTVDGSYWHAIMHRREPDYANA
                     RYWFRRVPQHAIYEPLAAAARELAAKAKLDGPAEFLAMQKSWDSFRFVDLCEAIARGR
                     SQCEQLAREVARAEWRLLFDHCYRAAIGS"
     gene            complement(7295..>8049)
                     /locus_tag="VFB80_07250"
     CDS             complement(7295..>8049)
                     /locus_tag="VFB80_07250"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN33597.1"
                     /translation="WYHVLNCGFPLKASGETDFPCMSSRSVGQGRTYVRLEQIERLDY
                     KPWCDGIRAGRSYVSDGYAHAVDFQVAGVRPGDGEVQLARASDVQVTARVAFAAQTPK
                     AVAYGGITPPAGREVVGDTVELHGPRYDEMVKGGQRLIEIVINGQVAASRQIPADGQI
                     HELKFTVPIRLSSWVALRHFPQLHTNPVNVLVGEQPIRASADSAQWCLDVIDLLWKNR
                     ERRIAEAERAEARAAYDRAIAKYRQIRDECAE"
BASE COUNT         1378 a         2681 c         2635 g         1355 t
ORIGIN      
        1 gccggacagc agtcttgccg cccgcactct gatcgccgca tcgcgatgct ctgccagcaa
       61 cttcagccgc acgggatcga gatcgccggc gctgacctgg ccctgttcca aggcgcctaa
      121 gagcgccagc gtccaggcgg gccgtgacag cagcacgtcg gtcgcctggg acttcaaccg
      181 tgggctgaag ccggcgaacc gcgagagcac cagttcggcg acgtcggccg cgtcatacga
      241 ggcaagcgtc gccagcgcgg cggactgaag atcggccgca accgccggcg caaacagccc
      301 ggcaaacagc ggctgctgcc cggcgaagct cgccagccgc agttgctcga ccgcggcgac
      361 gcgggccttg agcggcgcgt tttcgtccgc ggccttcgct gcggcctcgg caatcagcaa
      421 cttcatgagc gcctcggcct tgccgcccgt cgcggcggcg atctgctggg ccaggggtga
      481 accgggccgc gcggccagcc tttgcacgag cggaggcagc aatgtcgaat tctccttggc
      541 cagagccgcc agcgtcttca gcgtttcggc gacgtcgtcg ggccgctgct gcttgccgat
      601 ctgtgtcgcc aggctggcga gcagttcgcg gccttccttc gaatcgcgga aaccggcatc
      661 cgcggcgatc agcgacaagg ccccgcccgc gccttcgccg agcgagctga gcacggacac
      721 gcgcacgtag ccgtcggcgg cgtcgcgctg cgcgagttca accagtgccc gcgtgcgctg
      781 cgctgtcgcc ggcagctccc cgagcgagaa ggcgagctga tagcggacac gcagctccgg
      841 atcactggtc atcgccagca gccgctcgcg gatcgccgcc gacgaatcgg ctagcttctc
      901 agccagtcga acggcgtgct cgcggacgcg cggatgctcg tcggccaagc gcggcagcag
      961 cgccgcctcg ctcagcgctc ccagtccatc gagggcatac agggcgtgca gccgcgcggc
     1021 cggctccttc gattcagcgg cgagctttgt cagcggtgcg actgccgact tgtcttgccg
     1081 ctcgtagagc aaccgggcgg cggagtcgcg attccagcca ttcgcgtggg ccagcgtggc
     1141 aaccagttcg gaggtcgttg ccttaccgag ctggggcagc ggacgctgcc ggaagccgtc
     1201 gggcacgatc cggtagatcc ggccgcggtc gcggccgctg gtcaggtcga gatgcttctt
     1261 gatgagcggc ggcaggctcg cgggatgctc gatgacctcg cggtacatat cgaggatgta
     1321 gagcgtgccg tccggcgcgt tggcgaactg cacggggcgg aaccagatgt cgcgcgacga
     1381 gacgaactcg ctctgctgat cgatccgccg gccgaggaat ccgacgccgt ccgcttccag
     1441 gcgcttgcga tggacgaggt tgctgcctac gtcgccgatg atcgccaggc cgcgccactc
     1501 ggccggccag gcgtcgccgc gataaatcgt cgcgccggta gccccggtga aatagcccgc
     1561 cgggcggccg cccccttcga cgatgcccgg gacgatcttt tgggcccgca atcgggtgcg
     1621 gacaatccgc cagggttcga cggggctggt gcggaagacg tcggcctgcg gaccgtcggc
     1681 ggcgatgctc cgccgcggcg acggcgctgc caggtaggga ttccgcgcca aatagcggtc
     1741 ctcgtacatc acctgctgca agtggtcgct gttggagcag acaaagcggt cgccccagtc
     1801 gttgaacgag agcccgtgct gtgctccgcc gctgactggc gtcacttccc aagttcgcgg
     1861 atcgatggcg atgtcgcgcc cacgaatcgg cagcggcttg ctgtcgttct cgtctggccg
     1921 cacgagcgtt gccccactgc tgctgacggc agcgtaaatg cggttgtcga gaccccactg
     1981 gaacgagtta atcagccctt gcacgttgga gaggcccagg ccggtgtaga gcacccgccg
     2041 ctcgtcggcc ttgccgtcgc cgtccaagtc cttgagatag aagatgtccg gcgcgtcggc
     2101 gaccagcacg ccgcccgccc agcagaacac ggccgtcggc cagcccaacc cttcggcaaa
     2161 caccgtgctc ttgtcgaact tgccgtcgcc gttttcatcg accagcagcc gaacgcggct
     2221 aagtcgctcg tcgcggtttt cggaatagcc ccgcatctcg gcgacgtaca tccggccgtt
     2281 ttcgtcaaac gaaagggcga cggggctggc caagaggggc tccgcggcga cttgctcgag
     2341 cccgaatccg gcggagactt gaaaggtggc cagcgccgcc tgcggctcgg ttggagcgat
     2401 ccgcggcagc tctctggcat agtcgcggtc gatggagtcg ggctgggcgg cgctggggga
     2461 ggcaatcagg ccgagaatta atgggaccac taggacggat gggaccgttg tgaagggatg
     2521 gagcgctttc attttttggc gtgttatagc gttcaaaagt ccctgctggc cttgagttta
     2581 cagagcattc acggtacgac aatagtttac tacgctgcta cccgccacgg tcccgccgct
     2641 cgccgctacc ccgcagagtt tcatttgtcc gaacgcaaac aacccaattc tcaacccacc
     2701 tttcagagcc tcggcctgtc ggacgtcatg ctggacgccc tcgacctggc cggctatgag
     2761 tatccgacgc cgatccaggc cgggctgatc ccgctggcgc tgcaggagat cgacgtcgtc
     2821 ggccaggccc gcacgggcac gggcaagacc gcgtcgttcg cgatcccgat cctggagaag
     2881 ctccagctca agtccgacat ccatcacccg caggccctgg tgatggtgcc gacccgcgaa
     2941 ctggccgtgc aggtgaagga cgagatcgtc aagctctccg cgggccgccg ggtgcattgc
     3001 tgcgcggtct atggcggcaa gccgatccgc cagcagatcg aaaaactccg caagggggcg
     3061 cagatcgtcg tcggcacgcc cggccgcgtg ctcgaccacc tggcccgcgg caccgtcgat
     3121 ttttcgcggc tcaagttcgt cgtgctcgac gaggccgacc ggatgctcga catcggtttt
     3181 cgccccgaca tcgagcgaat cctgaagcgc tgccccgccg agcggcagac gctcttgctc
     3241 agcgcgaccg tgccgccgcc gatcgccaag ctggccgagc ggtacatgcg cgacccgcag
     3301 acgatcaact tctcgcccaa agacatcgcc gtcgagacaa tcgagcagtt ctatttcacc
     3361 gtcgatccgc agcgaaagtt cgacctgctg ctgcggctcc tgacccgcga gcagccgcgg
     3421 caggcgatcg tcttttgccg caccaagcgg ggaacggaga aggtctatct gcggctgagc
     3481 aagaaactgg ccgacgtgga agcaattcac ggggacctga accaggcgca gcgcgaccgg
     3541 gtgatggcgg cgtttcgcag cggcaaggtg aagattctcg tggcgaccga cgtgatcggc
     3601 cgcggcatcg acgtgacgag catctcacac atcgtcaact acgatatccc ggcgttctgc
     3661 gacgactatg tccaccgcgt gggccgcacc ggccgcatgg gccgcgaagg gatcgcattc
     3721 acttttgtgg cccccgacga aggctacgag ctgacgcgga tcgaagagcg gatcaaccgc
     3781 ctgctcaagc gggccgaaat gaaggatttt gaattccgct cccgcgaatc gctggccggc
     3841 gtgctacagg gcggcgacca tcacgaggga gatgctcacg acggcgaagg cgtgccggtc
     3901 gcggccgtag accgccctgc cccgccaccc ccgcccgcct cggcgatgcg ccggccgccg
     3961 aagaaatacc ggcgggcgct gtagggatcg ccctctgtgg cgttccgcgc ccgcgcggtg
     4021 tctgatcggc gcggcttacc gaaccgcggc aggcggtacg tagtctgcca ggcatggccc
     4081 gccaacttct cattctcgac ctggacgaaa cgctgttcta cgcggcgctc agtccgcgcg
     4141 gtcggccgcc cgattttgag ctggccggct attcggtcta tcgccgtccc tttttgcaag
     4201 agttcctgga ctttgccttt gagaattttg ccgtggccgt ctggtcgtcg gcgacggaac
     4261 cctatgtgcg ggcagcggcc cgcgaggtga tcgcgcgatc cgcagacttg caattcatct
     4321 ggtccatgga gcggtgcacg cggcgcttca acggcgagac gcgcgaggag tactgggtca
     4381 aggatctcaa gaagctgaag cggctgggat tctcgctgga ccgcgtgctg gtcatggatg
     4441 attctcctga gaagcatgag aggaattacg gcaatctcgt gccggtgcgg ccgttcttcg
     4501 gcgaccaggc cgataccgag ctccgcgaca tcacgccatt tctggaccgc ctgcaacgtt
     4561 gcgaggacgt tcgaaaaatc gagaaacgaa attggcggaa ttggctgtag gcagaaatcc
     4621 gcacggtgcg gtgttatcat cggcacttcg cagtctgagt acggagtacc gagtactcag
     4681 tgtcaatcac ttggcggctt ctcgattctc catcgcacac gatctatgac gctccgcatc
     4741 ctctgccttt ccgcactgct ggttcccgtg ctggcgctgg ccgatggccc gaccgataac
     4801 attcccgcga acgtccggcg gattccaaag ctgggaatcg aagtcccagc ggatcggcgg
     4861 gcaaagctgg aggcgggtct caaacagttg gaaataaaaa tcgccgccgt gcgcggcggc
     4921 caggatgcca aggccaaggc cctgctgccg gatgtcgagg tctatttcaa ggcggtacac
     4981 gacgcgctgg cctatcagga gttttttgcc ccagccgaaa tcgacttcgc cgatcgcgcg
     5041 ctggccaccg gccacgagcg ggtcgaccag ctcgcggcgg gccaagcgcc ctgggcggct
     5101 gccagcggcc tggtcgttcg cggctacgtc tccaaaatcg acagctccgt gcagccgtac
     5161 gggctggtcg tgcctgaaag ctatacgggc aagggcccgg ggcgttttcg ctgcgacctg
     5221 tggtttcacg gccgcggcga gacgctgagc gaactggctt tcatccgcga tcggacgaac
     5281 aacgtcggcg cgattgcgcc cgacgacacg atcgtcgtgc acccttacgg ccggtacagc
     5341 aacgccttca agttcgcggg cgagatcgac gtgctggagg ctctcgactc ggtgaagggc
     5401 cgctaccgcg tcgatgagga ccggattgcc gcccgcgggt tctcgatggg cggggcggcg
     5461 gcgtggcatt tcgccgtcca ctatcccgat cgctggttcg ccgccaatcc cggcgcaggt
     5521 tttagcgaga cgccggagtt cctgcgtacg tttcagcagg aggagctcaa gccggcctgg
     5581 tgggagaaga agctctggca gtggtacgac tgccccgagt ggtgccgcaa tctgcggcat
     5641 tgccctacgg tggcctacag cggcgaaatc gaccggcaaa agcaggccgc cgacgtgatg
     5701 gaaaaggctc tggccggcga ggagatcgag ctgacgcaca tcatcggtcc ccagacggcc
     5761 cacgcgattc acccgcagtc gaaggccgag atcgagcggc ggctggcgtc gctggccaag
     5821 atcggccggg acacgcggcc gctcgacgtg gatttcgtca ccagcacgct ccgctacaac
     5881 cgcctgcact gggtcaccat cgacgggctc gccgagcact ggtcgcgggc gtcggtctcg
     5941 gcctcgatcg cctcgacgca ggagctggag tttgaggcgg ccggcgtgat cgatattacg
     6001 ttctcgtttg ccgcgggcga ttcgccgttc gatctgggca cgaaggtcgt catcatcagc
     6061 ggcgagcaag agctggaagc gcccggcccg gcgagcgacc gctcgtggaa tgtctcgctg
     6121 caccgctctg cgggcgagtg gcgcctggga aagcggcccg ctgacgggct gcgcaagatt
     6181 cacggtctct ccggcccgat cgacgatgcc ttcatggact cgttcctgtt cgtgcggccg
     6241 accggcaaag ccgccaatga cgcggccggc aagtgggcgg cagcggaact ggagcgggcc
     6301 agcgagcact ggcggcggca cttccgcggc agggcccgcg tgaaggacga cactgccctc
     6361 acgcccgagg acatcgccgg cgcgaacctg gtcctgtggg gcgatccggg cagcaacgcg
     6421 gtgctcaagc agatcgccgg caagctgccg atccgctggg gcgaaaagca gatcgaagcg
     6481 ggtggccaaa aattcgccgc cgacacgcac gctcttgtag ccatctatcc caacccgctc
     6541 aaccccagcc gctacgtcgt gctcaacagc agcttcacgt tccgcgacta cgcctacctg
     6601 aacaacgccc gacaagtgcc gatgctgccc gactgggccg tggtcgatct caccacgccg
     6661 ccgaacagcg tctggcccgg caagatcgcc gccgccgatt tcttcgacga acggtggcag
     6721 ctcaagaacc aatagcagcc cggtagcagt gatcgaacag gagccgccac tcggcgcggg
     6781 ccacttcgcg ggccagctgc tcgcactgcg agcggccgcg ggcaattgcc tcgcacagat
     6841 cgacgaagcg aaacgagtcc caagacttct gcatcgcgag gaactccgcg ggtccgtcga
     6901 gcttcgcctt tgccgccagc tcgcgggccg cggccgccag cggctcatag atcgcatgct
     6961 gcggcacgcg gcggaaccag tacctggcgt tggcgtagtc cggctcgcgg cggtgcatga
     7021 tcgcgtgcca gtagctgccg tccacggtgt cgatatcctg gctgaggcag tgcgattcgt
     7081 ccaggaagtc gtgccacagc catagtccgc tgagtacgca gcgcgacgct acgaaatcga
     7141 cgatcttcgc gcccgccagc gaacgattga gagccaaggc cgtcaacttt ggccgcgcgc
     7201 cttcgttcgg cgtgcccggg ccgagctccg gcaaccgatc ttccaatagc acggcgatgg
     7261 ccggtccata ggcggttcgc tctgaatcca tcgattactc cgcgcactcg tcccggatct
     7321 gccggtattt ggcaatcgcc cggtcgtacg ccgcccgcgc ctcggcccgc tcggcctccg
     7381 caatccgccg ctcgcggttc ttccagagca gatcgatcac gtccaggcac cactgggcgc
     7441 tgtcggccga tgcccggatc ggctgctcgc cgacgaggac gttgaccggg ttggtgtgca
     7501 gctgcggaaa gtgccgcagg gccacccagc tgctcaggcg aatcggcacc gtgaacttca
     7561 gctcgtgaat ctggccgtcg gcgggaatct gccgggacgc cgctacctgg ccgttgatga
     7621 cgatctcgat cagccgctga ccacctttga ccatttcatc gtaccgcggg ccgtgtaatt
     7681 cgaccgtgtc gccgacgacc tcgcggccgg ccggcggagt gatgccgccg taggcgaccg
     7741 ctttcggcgt ctgagcggca aacgccacgc gggccgtcac ctgcacatcg ctcgcgcggg
     7801 ccagctgcac ttcgccatcg cccggacgaa cgccggcgac ttgaaagtcc accgcgtggg
     7861 cgtagccgtc gctgacgtac gaacggccgg cgcgaatccc gtcgcaccac ggtttgtagt
     7921 cgagccgctc gatttgctcc aaacggacgt acgtccgccc ctggccgacg ctgcggctgc
     7981 tcatgcaggg aaaatccgtc tcgccactgg ccttgagcgg aaagccgcaa ttcagcacgt
     8041 gataccagg
//
LOCUS       DASRJB010000270         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_77964, whole genome shotgun sequence.
ACCESSION   DASRJB010000270 DASRJB010000000
VERSION     DASRJB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77964"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFB80_07255"
     CDS             complement(<1..213)
                     /locus_tag="VFB80_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZN33598.1"
                     /translation="MNQPVAVVLAAGMGTRMKSDLPKVLVPVLGRPMIEYVLEALAAA
                     GIARSIVVIGYRAEDVRRLLAGRQGLE"
     gene            394..597
                     /gene="rpmI"
                     /locus_tag="VFB80_07260"
     CDS             394..597
                     /gene="rpmI"
                     /locus_tag="VFB80_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZN33599.1"
                     /translation="MPKQKTHKGTKKRFRLTASGKAKHRQAGTSHLASRKVKKTRRNL
                     RGTRVVSKTMLQQITRALAGHSS"
     gene            752..1108
                     /gene="rplT"
                     /locus_tag="VFB80_07265"
     CDS             752..1108
                     /gene="rplT"
                     /locus_tag="VFB80_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZN33600.1"
                     /translation="MRAMKGAARTKAKRRIFKRAEGFVGGRRKLYRTAKETLVRAGAF
                     AFRDRRARKRDFRRLWIIRLSAACRERDLRYSQFIAGLKAANIELDRKSLSEIAIADP
                     AAFDAVIAKVKAALKL"
     gene            1207..2289
                     /gene="pheS"
                     /locus_tag="VFB80_07270"
     CDS             1207..2289
                     /gene="pheS"
                     /locus_tag="VFB80_07270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZN33601.1"
                     /translation="MNLAQFLADLDGLLAEAQAAFDAAADPPALEAARIEFLGAAKGR
                     LKAVQKGLGAVAKEDKPAAGKRFNEVKSLVESAFEAAQQRATVAGPAAKGGSQFDPTV
                     PGAPLRLGRLHPITQTIEELKDIMGRLGFSAADGPEIEDEWHNFEALNIPLDHPARDP
                     LENFYLSVAASGQRQLADKVDSSEEPGQREVDAFRSPLLLRSQTSTVQIRVMEQRRPP
                     VRIISLGRVYRPDEADATHYPMFHQMEGLLVDRHVTMADLKSVLRMFATSYLGADVHV
                     RFRPSFFPFTEPSVEADMQWENRWIEFGGAGMVDPHVLTAVGYDPEVYSGFAFGLGVE
                     RLCMRRHGITDIREFYKNDVRFLAQF"
     gene            2369..>2655
                     /locus_tag="VFB80_07275"
     CDS             2369..>2655
                     /locus_tag="VFB80_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN33602.1"
                     /translation="MIVSWNWLNDLVPLKVTPAELVQRLMMAGLNHEGSEQLGSDWAI
                     DLEVTSNRPDCLGHIGIAREAAVLFNLQLKYSAADLTRARKNSPAASEPHR"
BASE COUNT          492 a          897 c          854 g          412 t
ORIGIN      
        1 ctccagtccc tggcggccgg cgagcaaccg tcgcacatcc tcggcccggt agccgatcac
       61 cacgatgctg cgggcaatcc ccgccgcggc cagcgcctcc agcacgtact cgatcatcgg
      121 ccgcccgagc accggcacca ggaccttcgg caggtccgat ttcatccgcg tgcccatccc
      181 cgcggctaaa acgacggcga ccggctgatt catgattgcg ttctcgtagc cgcttagccg
      241 cggccaagag gccacgtccg gcgtagtcgc cggagctaag caccgttgta atgcgtcccc
      301 cgcgggggca aagccccttt cacaatttgc cctagattcc tatactgcag ggctttccag
      361 caacaaagat gcccccggcg ggccgggttt gccatgccca agcaaaagac gcacaaggga
      421 acgaagaagc ggtttcgcct gacggccagc ggcaaggcca agcaccggca ggcggggacg
      481 agtcacctcg cctcgcggaa ggtgaagaag acccgccgca acctccgcgg cacgcgggtc
      541 gtcagcaaga cgatgctgca gcagatcacg cgagcgctgg cggggcatag cagctagggt
      601 agcaggcaca ccgagtgccg tcgtttgggg aaggacgctc aatcgtcaat gacggcacac
      661 ggagtgtgcc gactacatac cgcacgcggg cagccaacgc cgcagccgat gctgcggggc
      721 cttgccgtgc cacacaggaa ggacgcatgc catgcgtgcc atgaaaggtg cggcccgcac
      781 caaagccaaa cggcgaatat tcaaacgggc cgaagggttc gtcggcggac gccgcaagct
      841 gtatcgcacc gctaaggaga cgctggtccg cgccggcgca tttgcgtttc gcgaccgccg
      901 cgcccgcaag cgcgacttcc gccgcctctg gatcatccgc ctgagcgccg cctgccgcga
      961 gcgcgacttg cgctacagcc agttcatcgc cggcctgaaa gccgccaaca tcgaactgga
     1021 ccgcaagagc ctgtcggaga tcgctatcgc cgaccccgca gcgttcgacg cggtgattgc
     1081 caaagtcaag gcggctctca agctctagtg cgtgtatgag ccgggagcaa agctgctccc
     1141 ggttcgcggc ggctggaagc ccgccgcacg aaccgccgcc gcgccgcagt gtccgtgcat
     1201 gatgccatga acctcgccca attcctcgcc gatctcgacg gcctgctggc cgaggcgcag
     1261 gcggcgttcg atgcggcagc cgatccgccg gcgctcgaag cggccaggat cgaattcctc
     1321 ggcgcagcca agggccgcct gaaggccgtg caaaaaggcc tgggcgcggt cgccaaggaa
     1381 gacaagccgg cagccggcaa gcggttcaac gaggtcaagt cgctggtcga gtcggccttc
     1441 gaagctgcac agcagcgggc caccgtggca ggcccagccg ccaagggcgg ttcgcagttc
     1501 gatcccaccg tccccggcgc gcccctccgc ctcggccggc tgcatccgat cacgcagacg
     1561 atcgaagagc tgaaagacat catgggccgg ctgggcttct cggccgccga cggcccggaa
     1621 atcgaagacg agtggcataa tttcgaggct ctcaatattc cgctggacca tccggctcgc
     1681 gatccgctgg agaactttta tttgtcggta gcggcgagcg gccagcgtca gctggccgat
     1741 aaagtcgatt cgtcggaaga gccgggacag cgggaggttg acgccttccg ctcgccgctt
     1801 ctcctccgca gccagacgag caccgtgcaa atccgcgtca tggagcagcg gcggccgccg
     1861 gtgcggatta tctcgctggg ccgggtttac cggccggacg aggccgacgc gacccactat
     1921 ccgatgttcc atcaaatgga agggctgctg gtggaccggc acgtgacgat ggccgacctg
     1981 aagagcgtgc tgcggatgtt cgcgacgagc tacctgggcg cggacgttca cgtccgcttc
     2041 cgcccgtcgt tctttccgtt caccgagccc agcgtcgagg ccgacatgca gtgggagaac
     2101 cgctggatcg agttcggcgg cgcgggcatg gtcgatccgc acgtgctcac ggccgtcggc
     2161 tacgaccccg aggtctattc gggtttcgcc ttcggcctgg gcgtcgaacg gctctgcatg
     2221 cgccgccacg gcatcacgga cattcgggag ttttacaaga atgacgtgag gtttttggcc
     2281 cagttttgag agagggctag ggctcgtggg tagggctagg acagccgctg cccgccccta
     2341 accccagccc tagcccctag cccttcccat gatcgtttcc tggaactggc tcaacgatct
     2401 cgtcccgctc aaggtcacac cggccgagct ggtgcagcgc ctgatgatgg cggggctcaa
     2461 ccacgagggg agcgagcagc tggggagcga ctgggcgatc gatctggagg tgaccagcaa
     2521 tcggcccgat tgcctcggcc atatcggcat cgctcgcgag gcggccgtgc tgttcaactt
     2581 gcagctcaag tacagcgcgg ccgacctgac gcgggcccgc aaaaactccc ccgccgccag
     2641 cgagccgcac cgcat
//
LOCUS       DASRJB010000271         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1204997, whole genome shotgun sequence.
ACCESSION   DASRJB010000271 DASRJB010000000
VERSION     DASRJB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204997"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..636)
                     /locus_tag="VFB80_07280"
     CDS             complement(211..636)
                     /locus_tag="VFB80_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZN33603.1"
                     /translation="MSRIDSLAGALPPAADRRTNNIQDLDMNQFLKLMISELTNQDPL
                     NPMDNTQLVQQLGSIREIAANDKLSSTLTALQIEQSLTTASSLIGKEVAALTTDNEDI
                     RGVVDRVSVEVDPQKADKRTYRVHIGERQVELKNVREIK"
     gene            complement(661..2052)
                     /locus_tag="VFB80_07285"
     CDS             complement(661..2052)
                     /locus_tag="VFB80_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZN33604.1"
                     /translation="MQSTATTSVNILPVLDIGPPPGIARDPAPAGPFEVLVAPPPACP
                     PPPEPRLESANGNQPASDGEAVPDAADQTADPTSDSRPNGVSELEREDTAEIPPDSEA
                     ELIAASLAGAAAAVGSPAEAPAQAADGVEAGAEAMEPAATISGSAAIVGELPLGLPGA
                     VATEGTAGKPNSAQDASEQPAEAADRALAATSGDEPSPPGAELPEGGDDEQEIASEAS
                     QFTVNNSAAERPNQDETGKGESSHQQAADAAPSAAAPSDAAALSNSSELAPLASPEGN
                     RPTIETRAASPSPAPGIEAAGNSTPRRLPAAMLAPEPAVTRTPIAIDSARLLHRVARA
                     FTAAQLRDGEMQLRLSPPELGSLRLHVQLAEGALVARLEAETTTARAVLLDNLPALRE
                     RLAEQGLRIERFDVDLMQRQGSGTPDRPHDRESQTPQQPPVAVPRPVATNPTTSRPAV
                     PRTLGPSQLNVIV"
     gene            complement(2060..2755)
                     /locus_tag="VFB80_07290"
     CDS             complement(2060..2755)
                     /locus_tag="VFB80_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33605.1"
                     /translation="MKTALHWAGNLLIALSVGTFISLLIVLAMLWWRGALADERVLGM
                     LATLQGVKPAAPTPVPGQLDPDAEQPSIPQLLQTRLQASLDFDLRESAIDKALGDLRA
                     LETSIRTERSRLDGWKDDFDKRLARLETAATDAALLEVQQTLQAIAPKQAKDQIIKML
                     KDPVTAEDNPMQDVVTVLKAMPLDKRKKILGEFKTDPEIEILAEILRNIRLGSPDSDL
                     LRQTRDQLQQPNP"
     gene            complement(2752..3201)
                     /gene="fliJ"
                     /locus_tag="VFB80_07295"
     CDS             complement(2752..3201)
                     /gene="fliJ"
                     /locus_tag="VFB80_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HZN33606.1"
                     /translation="MPPFRLHTLLRLRIAERDERRADLAKALRAEEMLRAEQSRLAAE
                     QAELAQRERGLKSPGAANVDALLSTHRYELVLAAQARQLSSQLTQVAAEIERRRLVLV
                     EADRQVRVLEKLRERQAAAARIHEQRLDARRYDELAMIGHSRKEAAL"
     gene            complement(3230..>4202)
                     /locus_tag="VFB80_07300"
     CDS             complement(3230..>4202)
                     /locus_tag="VFB80_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HZN33607.1"
                     /translation="DRPRIDAPLSTGVRAIDGLLTCGLGQRIGIFSGSGVGKSVALGM
                     MARYTSADVTVVGLIGERGREVNDFVERDLGPSGLARSVVVVATSDQPALVRVQAAQA
                     ATTIAEYFRDQGKNVLLIMDSLTRFALAQREIGLAAGEPPTTRGYPPSVFALLPRLVE
                     RAGRAPRGSITALYSVLVEGDDVTEPVSDAVRGLLDGHIVLSRKLASQAHWPAIDVLE
                     SISRLFSEITPPEQRAAAQTLRELLAAYRDHEDLISIGAYRGGANPTVDAAIATRDEI
                     HRYLRQAIDESSNVETARADLLKLAQRCAAARKPAQQAPAAAAARRG"
BASE COUNT          583 a         1377 c         1463 g          779 t
ORIGIN      
        1 gttggccagg ttgttgccga cgacatcgac ctgcgtttcg gccgcctgca tgcccgtgag
       61 agcggtagtg agtgcggatg cgaggcccat attcagtcac ctatgaaaag gaatgcaatt
      121 cgaaatcgaa attccaagaa ccgaagatcc aaagaaccaa acccgcagtc gaataaccgc
      181 ttttggttct ttggttcttt ggcatacccg ttacttgatc tcacgaacgt tcttcagctc
      241 cacttgccgc tcgccgatgt ggacgcggta ggtgcgctta tcggccttct ggggatcgac
      301 ctcgacacta acgcgatcga cgacgccgcg gatgtcctcg ttgtcggtcg tgagggccgc
      361 gacctccttg ccgatcaggc tgctggccgt cgtcaggctc tgctcgatct gcagcgcagt
      421 cagcgtgctc gacagcttgt cgttggcggc aatctcgcga atgctgccca gctgctgcac
      481 gagctgcgtg ttgtccatcg gattgagcgg gtcctggttc gtcagctcgc tgatcatcag
      541 cttcaggaac tggttcatgt ccaggtcctg gatgttgttg gtccggcggt ccgcggcggg
      601 cggcagcgct ccggcgagcg agtcgatgcg cgacatggcg attcctttcg cgaggataag
      661 ttagacgatg acattgagct ggctgggccc tagagttcgc ggcacggccg ggcggctggt
      721 tgttggattg gtggcaacgg gccgaggcac cgccactggc ggctgctgcg gcgtttgact
      781 ctcgcggtcg tgtggccggt cgggcgtgcc gctgccctgc cgctgcatca gatcgacgtc
      841 gaatcgctcg atccgcagcc cctgctcggc cagccgctcg cggagcgcgg gcaggttgtc
      901 gagcagcacc gcgcgggcgg tggtcgtttc ggcctccagc cgtgcgacga gggctccttc
      961 ggccagttgc acgtgcagcc gcagcgagcc caattccggc gggctgagcc gcaactgcat
     1021 ttcgccgtcg cgcagctgcg ccgcggtgaa ggcccgggcg actcggtgga gcaggcgggc
     1081 cgagtcgatc gctatgggcg tacgagtgac cgcgggctca ggcgccagca tcgccgccgg
     1141 cagccgccgc ggcgtggaat tgcccgcggc ctcgattccc ggcgcgggcg atggggaggc
     1201 cgccctggtt tcgatcgtgg gcctgtttcc ttcagggctc gcgagcggcg cgagctcaga
     1261 ggaattcgat agcgccgccg catccgacgg cgctgcggcg ctcggtgcgg catcagcggc
     1321 ctgctggtgt gacgattcgc ccttaccggt ttcgtcctgg ttcggccgct ccgcagccga
     1381 gttattcacc gtgaattgtg aggcctcgct ggctatttcc tgttcgtcgt cgcctccctc
     1441 aggcagctct gcgcctggcg ggctgggttc gtcacccgag gtcgctgcga gcgcgcggtc
     1501 agcggcttca gccggctgct cgctcgcgtc ctgcgccgaa ttcggcttgc ccgcggtgcc
     1561 ctccgttgcg actgcacctg gaagtcctag cggaagctcg ccgacgatcg ctgcggaacc
     1621 ggatatcgtc gccgcgggtt ccatcgcctc tgcgccagcc tcaaccccgt cggctgcctg
     1681 ggccggagct tcggccggcg atccaaccgc cgcggcagct ccggccaacg atgcagcgat
     1741 cagctcggcc tctgagtccg gcgggatttc agcggtgtct tccctttcca gttcagaaac
     1801 gccgtttggc cgcgaatcgg aggtggggtc cgcggtttgg tcggccgcgt ccggcactgc
     1861 ttcgccgtca ctcgctggct ggttgccgtt cgcggactcg agccgcggct cgggaggcgg
     1921 tgggcaagcg ggcggcggcg cgacgaggac ctcaaacggg ccagcgggtg cggggtcgcg
     1981 cgcgatgccg ggtggcgggc cgatgtccag gaccggcaag atgttgacgc tggtggtagc
     2041 cgtggattgc acgtgtcgct caggggttgg gttgttgcag ctggtcgcgg gtctggcgga
     2101 gcagatcgct gtcggggctt cccagccgga tgttccgcag gatttccgcc aggatttcga
     2161 tctccgggtc ggtcttgaac tcgccgagga tcttcttccg cttatcgagc ggcatggcct
     2221 tgagcaccgt gacgacgtcc tgcatcggat tgtcctccgc ggtgacgggg tccttgagca
     2281 tcttgatgat ctggtccttg gcctgcttgg gcgcgatcgc ttgcagcgtt tgttgcactt
     2341 ccaagagggc ggcatcggtg gcagccgtct cgagcctggc cagccgtttg tcaaaatcgt
     2401 ccttccagcc gtcgagccgc gaccgctcgg tgcggatcga ggtctccagc gcccgcaaat
     2461 ctcccagggc cttgtcgatg gcgctctcgc gcaggtcgaa gtcgagactc gcctggagcc
     2521 gcgtctgcag cagctggggg atcgacggct gctcggcgtc cggatcgagc tggcccggga
     2581 ccggcgtcgg tgccgcgggt tttacgccct gcagcgtcgc cagcatcccc agcacgcgct
     2641 cgtcggccag tgcgccccgc caccagagca tggcgagcac gatgagcagc gagatgaacg
     2701 tgcctaccga caaggcgatg agcaggttgc cggcccagtg gagtgcggtc ttcacagcgc
     2761 ggcctccttc cggctgtggc cgatcatcgc cagctcgtcg taccgcctgg cgtccagccg
     2821 ctgctcgtgg attctggccg cggccgcctg ccgctcgcgg agtttctcca ggacgcgcac
     2881 ctgccggtcg gcctccacca aaacgagccg ccggcgttcg atttcggctg cgacctgcgt
     2941 cagctgcgac gagagctgcc gagcctgggc cgcgagcacg agttcatacc ggtgcgtgct
     3001 caagagggca tcgacattgg ccgcgcccgg tgatttcaga ccacgctcgc gctgggcgag
     3061 ctccgcctgc tccgccgcca gccgcgattg ttcggcccgc agcatttcct ccgcccgcag
     3121 cgccttggcc aaatcggccc ggcgctcgtc gcgctcggcg attcgcaggc gaagcagcgt
     3181 gtggaggcgg aaaggcggca cggagggttc agctttcggg gttcagggtt cacccgcggc
     3241 gagcagcggc tgcggccggt gcttgctgcg caggtttgcg cgcggcggca catctttgcg
     3301 cgagcttgag caggtcggcg cgggcggttt cgacgttcga cgattcgtcg atcgcctgtc
     3361 gcaggtagcg gtgaatctcg tcgcgcgtgg caatggcggc atcgacggtc gggttggccc
     3421 cgccgcggta ggcgccgatc gagatcaaat cctcgtgatc ccgataggcg gccagcagct
     3481 cgcgcagcgt ctgggcggcg gcccgctgct cgggcggcgt gatctcggaa aacagccggc
     3541 tgatgctctc cagcacgtcg atcgccggcc aatgggcctg cgaggcgagc tttcgcgaca
     3601 ggacgatgtg gccgtcgagc aggccgcgga ccgcgtcgct gaccggctcg gtgacgtcgt
     3661 ctccttcgac gagcaccgaa tacagcgccg tgatgctgcc ccggggagca cggccggcgc
     3721 gctctaccag ccgcggcagc agggcaaaca cgctgggcgg atagccgcgc gtcgtcggcg
     3781 gttcgccggc agccaggcca atctcgcgct gggcgagggc gaaccgcgtg agtgagtcca
     3841 tgatcagcag gacattcttg ccttggtcgc gaaagtattc ggcgatggtg gtggcggcct
     3901 gcgccgcctg cacgcggacc agcgccggct gatcgctcgt ggcgaccacg acgacgctgc
     3961 gcgcgaggcc gctcggaccc agatcgcgct cgacgaagtc gttcacctcg cggccccgct
     4021 cgccgatcag gccgacgacc gtgacgtcgg ccgacgtgta gcgggccatc attcccagcg
     4081 ccacgctctt gcccacgccg gagcccgaga agatgccgat ccgctgcccc aagccgcagg
     4141 tgagcaagcc atcgatcgcc cgtacgcccg tcgaaagcgg cgcgtcgatc cgcgggcgat
     4201 cg
//
LOCUS       DASRJB010000272        11962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4027074, whole genome shotgun sequence.
ACCESSION   DASRJB010000272 DASRJB010000000
VERSION     DASRJB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4027074"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1011
                     /locus_tag="VFB80_07305"
     CDS             349..1011
                     /locus_tag="VFB80_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZN33608.1"
                     /translation="MSLRLLSLAFVALAVPAFAADSITGTYMESRTCQVYTGPCFANA
                     ETAMAGKEAVMAWNIESGKRGAVDLKGLSVVMVVRGDDMFGHQGLAEPKKVQSAIIVD
                     DRASAEQRAALVEFAKEHTGQAGRGVIRVDAAPIKMSMDSVELKGELKAGEVVTLKTR
                     KTKPGDCICSNEIAFYPPLAQLERFAPGVSIESEFKGRAFGATWSNPDSRSAYMGEFV
                     YE"
     gene            1019..1375
                     /locus_tag="VFB80_07310"
     CDS             1019..1375
                     /locus_tag="VFB80_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33609.1"
                     /translation="MQLTVEVPGELAPELSAAGDENLGQILALGLREWRTRRGAEFPG
                     LNALIERLAQLPDPQDVLALRPTPEMQQRADDLLAKSRNGGLTPAEQLEWKQLEFAEH
                     IVRMAKAHAALKLQRN"
     gene            complement(1846..2376)
                     /locus_tag="VFB80_07315"
     CDS             complement(1846..2376)
                     /locus_tag="VFB80_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33610.1"
                     /translation="MVAYWPLAVGLGVLVAAAVLNQVNGRVPNLLSLGMIGAAWLAAL
                     FIQCGLAPTSGGGLAASVICTFAALAVLVPDYAIGCVPAGCVKAQMAFAAWIGCALGL
                     GPALTATIIATLAGELVTAAAVALYKIRQNSQAPELYQRPIDLIRPPALFPIQTTLSA
                     GSLLGIAAAALLGYVA"
     gene            complement(2513..3697)
                     /locus_tag="VFB80_07320"
     CDS             complement(2513..3697)
                     /locus_tag="VFB80_07320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN33611.1"
                     /translation="MDPFLTLMQIAGLVVGGMCHALLGSIKVPLAGKLQIDEAKVGGL
                     VGIFGFTLIPTVLAAGFLVDALGQKAVLTGGLALLTVSLVLLSQARSYPVALVAVLLL
                     GIGWSALVNVLNVTSPPAFLPLEKTARHMAYAMNLGDFIFGMGAFATPLLIAALVPRI
                     RLERTLLVPAAITILPLVLAYGVDWGRLIVTHEQTVSGGLGVLLSDPIVWLCCAAFFC
                     HVPIEASVAAWATTLMTGRGVSEATAATLLSVFWLTFTGSRLLTALLLPAGADTILVA
                     AMAGLCIAFTVGLALSRSASLTCGLVVAAGLILGPIFPTLIAILMSHVTPSLHGRVVG
                     IFFCIGGIGWTAIPILIGAYAKRTNVQRAFLIATLCAVLLTGLSLALHGALAGRHGSP
                     GG"
     gene            complement(3720..5111)
                     /locus_tag="VFB80_07325"
     CDS             complement(3720..5111)
                     /locus_tag="VFB80_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33612.1"
                     /translation="MLAGFSTPQSRRRNSRRDFLALGALGGLSFGWPAANCAAAGRAG
                     GAAFGRAKRCLLLFLTGGPPQHDTWDLKPEAPAQIRGELKPIRTNVPGIQVSELFPKL
                     AGQIDKLRIVRSVSHADTVHTSAGYTMLTGAHHPLANSASASLIRPTADDLPPLGSWL
                     ARARAGRGGLPFVSLPEVIKDANVNTFPGQDAGLLGQRFAPLRIEANADRTGFGVPPI
                     VLPADVSLERLAERRRLARVLAGQGSELDAGPVATPLAAQRLRAAELLASTNLAQAFD
                     LGREPAGRREAYGSHLFGRGCLLARRLLEAGVGLVTVYWHYEGPDDSPVWDTHWNNFQ
                     HLRQRLAPPTDQALAALLDDLADRGLLDDTLVVCLGEFGRSPKINGKAGRDHWPHVQS
                     ILLAGAGIPGGTVYGASDRDGAYPRDQAISPPSLMATLLHLLGVPADLEAIDRSGRPL
                     RAYGAAPLPELIA"
     gene            5301..5939
                     /locus_tag="VFB80_07330"
     CDS             5301..5939
                     /locus_tag="VFB80_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33613.1"
                     /translation="MSDITHLLGQAENGVPRAAEQLLPLVYDELRKLAAARLAQERPG
                     QTLQATALVHEAYLRLVKPGVGDRESGVGENLLPATPDPRSLTPGFDSRGHFFAAAAE
                     AMRRILIEQARRRGSQKHGGGRGRQPLEHVEIAAPEPSLDIVALDEALEKFERVDPLK
                     AQLVKLRYFAGLTIPQAAEALGISSTTADRYWAYARAWLHTELKRGDETQAR"
     gene            6010..9150
                     /locus_tag="VFB80_07335"
     CDS             6010..9150
                     /locus_tag="VFB80_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33614.1"
                     /translation="MTSKCQAEEAIFLAALEITVGTERESYLQTACAGDMALLQRLRE
                     LLAAHVDEDGPLDATRPPLDPPPVEEGPGTRIGPYKLLQVIGEGGMGVVYLAAQVEPV
                     ERQVALKIIRPGMDTRQVVARFEAERQALALMDHPNIARVLDGGTVGGLGPGARAREG
                     RLESDHPGSESLALAPSPSPSAGRPYFVMELVKGVPITQYCDDRQLPLRRRLELLVPV
                     CQAIQHAHQKGVIHRDIKPTNVLVAEYDQLPVPKVIDFGVVKALGQRLTEQTLFTEFG
                     QVVGTVEYMSPEQARLNQLDVDTRSDIYSLGVLLYELLTGTTPFERHRLKTAAFDEVL
                     RIIREEEPETVSKRLARTRSVQVLNAESGKRIERKHSELRAPRWFELDWIVMKALEKD
                     RNRRYDSAQALASDLLRYLNDEQVAACPPSVLYRAGKFARRHRRTLATAMLLGVVSLA
                     ALAGVTLSVGWALRDREAVAESAAREKRDRERQIELERSGRQSRLEADLAKALVDVEQ
                     LYRRDRVSDALAAITQTEALLTAEASAQQRELAGQWRRDLELAWRLEELRFQRLKVMD
                     DDLDLKSAEEAWREAFASYGIDVTQLPPEVAGTRIRSSRICFHLVAALDEWHLSTFQK
                     KSDRPLLEITLLADPDPWRVRLREAAQRGDANVLEEMSRQQELLGQPPATITVLALAL
                     ARCQQKTRCVEVLKLGQRRNPNDLWLNVYLTTSLMNLKPAQPAAAEAYCRAAVTLRPD
                     SGSLRLLLARTLQTQQRLTEAEEECRESIRLNPERIDNHLALGQILFLLKRFGDAEFE
                     FREVVRIKPNYYRGYMQIGGMLMMTDADRLPEAEANLREAAKLHPDDPIVLQGLGRVQ
                     TLQRKNTDGIATYERLVKIAPHIGAHHNELARLYEAEGRWREALDGYTAATRASPNFL
                     QPHLRLADLLTSCPARELRDVQRAIAAAKKATELDPNVETSWQCLGIAYRVAGNGEAS
                     LEALNKSLALRKGPSAESPLLILNLALAYLQTGDRTKAQEHYDRAAPVVRRARAMTSQ
                     MRETWSEAEAQFQAPP"
     gene            9228..>11962
                     /locus_tag="VFB80_07340"
     CDS             9228..>11962
                     /locus_tag="VFB80_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33615.1"
                     /translation="MSQSHSPEEAIFLAALEQGSRQARESYLAAACGGDKALLARVRE
                     LLAAHDNDSGPLDLTLPGLKSGTVRETIERPGTQIGPYKLLQVIGEGGMGVVYMASQR
                     EPVERTVALKIIKPGMDTRQVVARFEAERQALALMDHPNIARVLDGGSVGTEGLGLGA
                     RAREGRSESDRTGSESLALATSPSPSSGRPYFVMELVKGVPITQYCDEHHLTPRQRLE
                     LFVPVCQAIQHAHQKGIIHRDIKPTNILVAEYDQQPVPKIIDFGVAKAIGQRLTEKTM
                     FTEFGQVVGTVEYMSPEQAKLNQLDIDTRTDIYSLGVLLYELLSGETPFDRQRLRSAA
                     FDEVLRIIREEEPPRPSTKLSSSHSLPSIAASRHTEPRQLASLLRGELDWIVMKALEK
                     DRNRRYETANGLAMDLLRYLHDEPVLACPPSIGYRVGKFARRNRRSLVTAALFGVLLL
                     GAIGAIASIAGWAARDRTAQRAAAAQRIALDRAERRIVLEREIEAALGEVALEYSHDR
                     LAHAASGQKRAASLWASGASDPALKQRIEQWRVDLQLAQRLDEIRLEQAAVKDGHYAR
                     AAADPVYSEAFQEYGLDFDKLADAALASRVQSSAIRDRLIPALDDWLETRLIGQLSGD
                     KLVAVAAAADGDPWRNRLRQASLKSDPKELLALAQDADVLEHPPTSLLLLASLLRGSG
                     QLQAAIALLEQAQQQYPVDFWTNQNLGLYLMQLPMPPAEKAIGYYRAAVTLRPTSPGV
                     RVNLGNALRRLNRPAEAEREFRTAIELKPDYAEAHLNLGTALHDLGLLDEAEREFLIA
                     LENRPNYAKALANLGNIYQIEGRLIEAEFCCRLAVAIDPDDLFAQKTCGDVLLKLDRY
                     AESEAAYREVVRLDPRAGRPHQDLGTVLRLQGKLNDAAAEFQLALRLDPRLGQSQS"
BASE COUNT         2240 a         3871 c         4014 g         1837 t
ORIGIN      
        1 ccaggcccaa atcttgcctg ggatatgccg ggccgccgcc agcgccgccc ccgacagcat
       61 caagatcaaa ccgcccgaga aaaactgatt gctggccagc tgccggctaa gaaaatccca
      121 gagtccatcc atctcagact ataaaactcc aagaaccgaa gaaccaaaga accaaggccg
      181 caagaaagct gattcgccga gggcggccga ttattcccgg cggagaaaga attgccgaac
      241 cgcgaagcct gcattccagt ggcccctgag ccgcggctgc tatagaatcg agggtggctg
      301 attcgtccgc tctaccgcca ttcttcctgc ccagttggag gttttgccat gtcgcttcgt
      361 ttgctgtccc tggcctttgt cgcgctggcc gtccccgcct tcgcggctga ttcgatcacc
      421 ggcacctaca tggaaagccg cacctgccag gtttataccg ggccgtgctt tgccaatgcc
      481 gagacggcga tggcgggcaa ggaggcggtg atggcctgga acatcgagtc gggcaagcgg
      541 ggcgcagtcg atttgaaggg gctctccgtg gtgatggtcg tccgcggcga cgacatgttc
      601 ggccaccagg gcctggccga gcccaaaaaa gtgcagtcgg cgatcatcgt cgatgaccgg
      661 gcgtccgccg agcagcgggc cgcgctggtg gaattcgcca aggagcacac cggccaggcc
      721 ggccgcggcg tgattcgcgt cgatgccgcg ccgatcaaga tgtccatgga cagcgtcgag
      781 ctgaagggcg agctgaaggc cggcgaggtc gtgacgctca agacccgcaa gacgaagccg
      841 ggcgactgca tttgcagcaa cgagatcgcc ttctatccgc cgctggccca gctcgagcgg
      901 ttcgcccccg gcgtgtcgat cgagtccgag ttcaagggcc gcgccttcgg cgcgacctgg
      961 agcaaccccg acagccgcag cgcctacatg ggcgagtttg tgtacgaata gggcgatcat
     1021 gcaactcacg gtggaagtcc ctggcgagct ggcgcccgag ttgtcagctg ccggcgacga
     1081 gaatctgggg caaatcctgg ctctgggtct gcgcgaatgg cggactcgac ggggcgcgga
     1141 gtttccgggg ctcaacgcgc tcatagaacg cctggctcaa ttgcccgatc cacaggacgt
     1201 gctggccctg cgaccgaccc cggaaatgca gcagcgtgcg gacgatctcc tggctaagtc
     1261 gcgaaacggc ggtctcactc ccgccgagca attggaatgg aagcagttgg agttcgccga
     1321 gcatatcgtg cgaatggcaa aggcgcacgc agccctgaag ctgcaacgca attgaccgtc
     1381 gatgtcgcag tcccatgttc ccaatgagct gcgacgactg gtcagagatc gagcccgcga
     1441 ctgctgtgag tattgcctga ttccacagtc attttccctg ctcgcgtttt ggatcgacca
     1501 catcgaggcc gaaaagcacg gcggggcaac gcacgactcg aatctcgcgt ggacctgtat
     1561 cttgtgcaac cagcacaagg ggacggacct cacctcgctt gacccaacca ctggcgctat
     1621 tgagcgtttg tttcatccgc gacgggacca ctggtccgac cattttcgac ttgccgacgg
     1681 gcggatcgag ccgctgaccg cctcaggccg cgcaaccgct cgcctcttgc aattcaatct
     1741 tcctgaacga atcgccgaac ggctggtcct tgccaggtcg ggaaacctcg ccccaggcgg
     1801 ccaggccagg ccgaaggagc gcgatcagta gttcggagct gtcgatcagg cgacgtagcc
     1861 cagcagagca gccgcggcga tgcccaacag cgagccggcc gagagagtcg tttgaatggg
     1921 gaacagggcg ggtggccgga tgaggtcgat gggccgttga tagagctctg gggcctgcga
     1981 gttctggcgg atcttgtaga gcgcaacggc ggccgcagtg acgagctcgc cggcgagcgt
     2041 ggcgatgatg gtcgcggtca aggccggtcc caggcctagt gcgcagccga tccacgcggc
     2101 gaaggccatc tgcgccttga cgcagccggc gggtacgcag ccgatcgcgt agtccggaac
     2161 gagcaccgcc agggcggcga aggtgcaaat cacgctggcg gcgaggccgc cgcccgaagt
     2221 cggcgccaga ccgcattgga tgaacagcgc cgccagccag gccgcgccga tcattcccag
     2281 cgacagcagg ttgggcaccc ttccgtttac ttgattgagc accgccgcgg cgaccaagac
     2341 acctagtcca acggcgagcg gccaataggc aaccatgcga gcagccctcc tttagggaaa
     2401 gctaactccg cgctcgtggc cgggtcgtcg aacttgcagt ttcgctggcc cggaaggttc
     2461 ccgcgaaaca gggcgtagca attgtcgcga ttcgttgggc aaaccctctc gatcagccgc
     2521 caggcgatcc atgccggccg gccaaggcac cgtgcaaggc caggctgaga ccggtgagca
     2581 gcaccgcaca cagcgtcgcg atcagaaagg cccgctgcac gttcgtccgc ttggcgtagg
     2641 ccccgatcag gatcggaatc gcggtccagc cgatgccgcc gatgcagaag aagatgccca
     2701 ccacgcgtcc gtgcagcgaa ggcgtcacgt ggctcatcag gatcgcgatc agcgtgggga
     2761 agatcggccc caggatcaaa ccggccgcga ccacgagccc gcacgtcaag ctggccgagc
     2821 ggctgagggc caggcccacc gtaaacgcga tgcacaagcc cgccatcgcc gcgaccagga
     2881 ttgtgtcagc gcccgcgggc aggagcagcg cggttagcag ccgcgatccg gtgaaggtca
     2941 gccagaatac cgacaacagc gtggccgccg ttgcctcgct gacgccgcgg ccggtcatca
     3001 gcgtcgtcgc ccaggcggcc acggacgctt cgatcggcac gtgacagaag aacgcggcgc
     3061 agcagagcca gacgatcggg tcggagagga ggacacccag gccgccgctc acggtctgct
     3121 cgtgcgtgac aatcagacgc ccccagtcca cgccatacgc cagcaccagc ggcaggatgg
     3181 tgatcgccgc cggcacgagc agcgtccgct ccaggcgaat ccgcgggacc aacgccgcga
     3241 tcaatagtgg cgtcgcgaaa gcgcccatgc cgaaaatgaa atcgcccagg ttcatcgcat
     3301 aggccatgtg cctggcggtt ttttccagcg gcagaaaggc gggcggcgag gtgacgttga
     3361 gcacgttgac cagcgccgac cagccgatgc ccagcagcag gaccgcaacc agcgcgactg
     3421 gataggagcg agcctgcgac agcagcacga ggctaacggt cagcaaggcc aggccgccgg
     3481 tgagcacggc cttctggccg agggcatcga ccagaaaccc cgcggccagg accgtgggga
     3541 tcagcgtgaa cccgaagatg ccgaccaggc cgccgacctt ggcctcgtcg atttgcaatt
     3601 tgcccgccag cgggaccttg atgctgccga gcagggcgtg acacattccg ccgacgacca
     3661 gtccggcgat ttgcatcagc gtcaggaatg gatccataga gggcccgagg tgaatcgact
     3721 taggcaatga gttccggtaa gggtgctgcg ccgtaggctc gcagcggccg gcccgaacgg
     3781 tcgatggctt cgagatccgc tgggacgccg agcaggtgca gcagcgtggc catcaggctc
     3841 ggcggcgaga tcgcctggtc gcgcggataa gccccgtcgc ggtccgacgc cccatagacc
     3901 gttccacctg ggatccccgc gcccgcgagc aggatcgact gcacgtgcgg ccagtgatcg
     3961 cggcccgcct tgccgttgat cttgggcgag cggccaaatt cgcccaggca gacgacgagc
     4021 gtgtcgtcga gcaggccgcg gtcggcgaga tcgtcgagca gcgcggccag cgcctgatcg
     4081 gtaggcggtg cgagccgctg gcgcaagtgc tggaagttgt tccagtgcgt gtcccagacg
     4141 ggtgaatcgt ccgggccttc atagtgccaa tagaccgtga ccagacccac tccggcctcc
     4201 agcagccgcc gggccagcag gcagccgcgc ccgaacaggt gcgagccata agcctcgcgg
     4261 cggccggcgg gctcgcggcc caggtcgaag gcctgggcga ggttggtgga tgcgagcaat
     4321 tccgccgccc gcaggcgctg ggcggcgagc ggcgtcgcta cgggcccggc atccagctcg
     4381 ctcccttgtc cggccaggac gcgggccagc ctgcggcgct cggccaggcg ctcgagcgag
     4441 acgtcggcgg gcaacacgat cggcggcacg ccgaagcccg tgcgatccgc attggcctca
     4501 atccgcagcg gagcaaagcg ctgtcccagc aggcccgcat cctgcccggg aaacgtgttg
     4561 acgttggcat ccttgatgac ctccggcagc gacacaaacg gcaaaccgcc gcggcccgcg
     4621 cgggccctgg ccagccagga ccccagcggc ggcaagtcgt cggccgtcgg tcggatcagc
     4681 gacgcgctgg cggaattggc gaggggatga tgcgcaccgg tgagcatcgt gtagccggcc
     4741 gacgtgtgga ccgtgtcggc gtgcgagacg ctgcgcacga ttcgcagctt gtcgatttgg
     4801 ccggccagtt tcggaaacag ctcgctgacc tgaatgccgg gaacatttgt gcgaatcggc
     4861 ttcagctcgc cgcggatttg cgcgggcgcc tcgggcttca ggtcccacgt gtcgtgctgc
     4921 ggcggtccgc cggtcaggaa gagcaacaga cagcgcttgg cccggccgaa ggcggcgccc
     4981 cccgctcgac cagcggcggc gcagttcgca gcaggccagc caaacgacaa tccgcccagc
     5041 gctcccagcg ccagaaagtc gcgccgagaa ttccggcggc gggactgtgg cgtggaaaat
     5101 ccagcaagca tggcgtggcg gtcgcgggac gcgcgactct cccgcccgaa gattctagcg
     5161 cgaccaagcc ccagttacca caaactctcg ggaagttggc cggtcaagta agaaattccg
     5221 gatttactcg caattggctc gcctacatgc cacaatagcc ggcccaacgt cgcccgatga
     5281 acgcaattcc gagctgccgc atgtctgata ttacgcatct gctaggtcag gccgagaatg
     5341 gagttccccg cgccgccgag cagctgttgc cgctggtcta tgacgagctg cgaaaactcg
     5401 ccgcggcccg gctggcccag gagcgcccgg gacagacgct ccaggcgacg gcgctggtgc
     5461 acgaggcgta tttgcggctg gtaaagccgg gggtcgggga tcgggagtcg ggggtcggag
     5521 agaatttgct tcctgcgact cccgaccccc gatccctgac ccccggtttc gattcccgcg
     5581 gccacttctt cgctgcggcg gccgaggcga tgcggcggat tctgatcgag caggcccgcc
     5641 gccggggaag ccagaaacac ggcggcggac ggggccggca gccgctggag cacgtcgaaa
     5701 ttgccgcgcc cgagccttcg ctggacatcg tggccttgga cgaagccttg gagaagttcg
     5761 agcgagtgga cccgctcaag gcccagctgg tcaagctgcg gtactttgcc gggctgacga
     5821 ttccgcaagc cgcggaggcc ctcggcattt cgtccacaac tgccgaccgc tactgggctt
     5881 acgcacgggc ctggctccac accgagttga aacgcggtga cgagacccaa gctcgctaag
     5941 cgatcaacca gattttttgg ggtctttggc ggccgttttt cgcatggatg aaataagccc
     6001 cggtgaatca tgacctccaa gtgccaagcc gaagaagcca tcttcctggc ggcgctggaa
     6061 atcacggtcg ggaccgagcg tgagtcgtat ttgcagacgg cctgcgccgg cgatatggcg
     6121 ctgctccagc ggctgcgcga actattggcg gcccacgtcg acgaggatgg cccgctcgac
     6181 gccacgcggc cgccgctcga tccgccgcca gtcgaagaag gacccggtac gaggatcggc
     6241 ccctacaagc tgctgcaagt gatcggcgaa gggggcatgg gcgtcgtcta tttggccgct
     6301 caggtcgagc cggtcgagcg gcaggtggcg ctcaagatca tcaggccggg aatggacacg
     6361 cggcaggtcg tcgcccgctt cgaagccgag cggcaggccc tggcgctgat ggaccatccg
     6421 aacatcgccc gggtgctgga tgggggaacg gtgggagggc tagggccagg ggctagggct
     6481 agggaaggca gactagagtc cgatcaccct ggatctgaat cactagccct agcccccagc
     6541 cctagccctt ccgccggccg gccctacttc gtcatggagc tcgtcaaggg cgtgccgatc
     6601 acgcagtatt gcgacgatcg gcagctgcct ttgcggcggc ggctggagct gctggtgccg
     6661 gtctgccagg ccatccagca tgcccatcaa aaaggcgtca tccaccgcga catcaagccc
     6721 accaacgtgc tcgtcgccga atacgaccag ctgccggtgc ccaaggtcat cgatttcggc
     6781 gtcgtcaagg cgctgggcca gcggctgacc gagcagacgc tgttcacgga attcggccag
     6841 gtcgtcggca ccgtggagta catgagcccc gagcaggccc ggctcaacca gctcgacgtc
     6901 gatacgcgga gcgacatcta ttcgctgggc gtactgctgt acgagctgct cacggggacc
     6961 acgcccttcg agcgccaccg gctgaagacg gcggcgttcg acgaagtgct gcggatcatc
     7021 cgcgaagaag agccggagac ggtgagcaag cgattggcgc gaacgcgcag cgtccaagtg
     7081 ctgaatgcgg aaagcggaaa gcgaatcgaa cggaagcatt ccgaactccg cgctccgcgc
     7141 tggtttgagc tggattggat cgtgatgaag gctttggaaa aggaccgcaa tcggcggtac
     7201 gactcggccc aggccctggc cagcgacctg ctgcggtacc tgaacgacga gcaggtagcg
     7261 gcctgcccgc cgtcggtgct gtatcgcgcg ggcaagtttg cccggcggca tcgccggacg
     7321 ctggctaccg cgatgctgct gggcgtcgtg tcgctggcgg cgctggcggg agtgaccttg
     7381 agcgtcggct gggcgctgcg cgatcgcgag gccgtggccg agagcgccgc ccgggaaaag
     7441 cgcgaccgcg agcggcaaat cgagctggag cgctccggcc gccagtcgcg gctcgaagcc
     7501 gatctggcca aggcgctggt cgatgtggag cagctctatc gccgcgatcg ggtgagcgac
     7561 gccctggcgg cgatcacgca aaccgaagcc ctgttgaccg ccgaggccag cgcccagcag
     7621 cgggagctcg ccggccagtg gcggcgcgac ttggagctcg cctggcggct ggaggaactg
     7681 cggttccagc ggctcaaggt gatggacgac gatctcgatc tgaagtccgc cgaagaggcc
     7741 tggcgggaag cgtttgccag ctatggcatc gacgtgacgc aattgccgcc cgaagtggcc
     7801 gggacccgca ttcgctcgtc ccgcatctgc tttcaccttg tggctgcgct cgacgagtgg
     7861 cacctgtcga ctttccagaa gaagtccgat cggccgctgc tcgaaatcac cctgctggcc
     7921 gatcccgatc cctggcgcgt gcggctgcgg gaagccgccc agcgcggcga tgcgaacgtg
     7981 ctcgaggaaa tgtcgcggca gcaggaattg ctggggcagc ctcccgcaac gattaccgtg
     8041 ctggccctgg ccctggcccg ctgccagcaa aagaccaggt gcgtcgaggt gctcaagctg
     8101 ggacagcgcc ggaaccccaa cgatttgtgg ctcaacgtct acctgaccac cagcctgatg
     8161 aatctaaagc ccgctcagcc ggcggcggcg gaggcctatt gtcgcgcggc agtgacgctc
     8221 cggcccgata gcggcagttt gcggctgctg ctggcccgca cgctgcaaac gcagcagcga
     8281 ttgaccgagg cggaggagga gtgccgcgaa tcgatccgcc ttaacccgga gcggatcgac
     8341 aatcacttag cgctgggcca gattctgttc ctgctcaagc gctttggcga cgccgagttc
     8401 gagttccgcg aagtcgtgcg gatcaagccc aactactatc gcggctacat gcagatcggg
     8461 ggcatgctga tgatgacgga cgccgaccgg ctgcccgagg ccgaggccaa cctgagggaa
     8521 gccgcgaagc tgcatcctga cgatccgatc gtgttgcagg gcctcggcag agtgcagacg
     8581 ctccagcgca aaaatacaga cggtatcgcc acctacgagc gtctcgttaa gatcgcgccc
     8641 cacatcggcg cccaccacaa cgaattggcg cggttgtacg aggctgaggg ccgctggcga
     8701 gaagcactcg acggctacac cgcggcgacc cgcgccagcc ccaactttct gcagccccat
     8761 ttgcggctgg ccgatctgct gacgagttgc ccggcgcgtg agctccgcga tgtgcagcgc
     8821 gccattgcgg cagccaagaa ggccacagaa ctggatccga atgttgagac ttcgtggcaa
     8881 tgtttgggta tcgcctaccg ggtcgcaggc aacggcgagg ccagcctgga ggcgctgaac
     8941 aagtcgctag ccctgaggaa aggcccgtcc gccgaaagcc cgctcttaat actgaacctg
     9001 gccctggcgt acctccagac cggcgatcgg accaaggccc aggaacatta cgatcgcgcg
     9061 gcgcccgtcg tgcgccgcgc ccgggccatg accagccaga tgcgggaaac gtggtccgaa
     9121 gccgaggcgc aattccaagc cccgccatga tttgtgcaaa acggagacca cggtggggtg
     9181 atttgggagc cgatttcgca ttgatggcta ggcgcacgag gtgaaccatg tcgcaatctc
     9241 actcgcccga agaggccatc tttctggcag ccctggagca aggcagccgg caagcccgcg
     9301 agtcgtacct tgcggcggcc tgcggcggcg ataaggcgct cttggcgcgg gtccgcgaat
     9361 tgctcgccgc tcacgacaac gacagcggcc ccttggacct gaccctgccg gggctgaaat
     9421 cgggcaccgt ccgggaaacg atcgagcggc ccggcacaca gatcgggccc tacaagctgc
     9481 tgcaagtgat cggcgaaggc ggcatgggcg tggtctacat ggccagccag cgcgagccgg
     9541 tcgagcgcac cgtcgcgctc aagatcatca agccgggcat ggacacgcgg caggtcgtcg
     9601 cccgcttcga agccgagcgg caggccctgg cgctgatgga ccatccgaac atcgcgcgag
     9661 tgctggatgg gggatcggtg ggaacggaag ggctagggct aggggccagg gctagggaag
     9721 gcagatcaga gtccgatcgc actggatctg aatcactagc cctagccacc agccctagcc
     9781 cttcctccgg ccgtccctac ttcgtcatgg agctcgtcaa gggcgtgccg atcacgcagt
     9841 actgcgacga gcaccacctc acgccgcggc agcgcctgga actgttcgtg ccggtttgcc
     9901 aggccatcca gcacgcccat cagaaaggca tcatccaccg cgacatcaag ccgacgaaca
     9961 tccttgtcgc cgaatacgac cagcagccgg tgcccaagat catcgacttc ggcgtggcca
    10021 aggcgatcgg ccagcggctg accgagaaga cgatgttcac ggagttcggc caggtcgtcg
    10081 gcaccgtgga gtacatgagc cccgagcagg ccaagctcaa ccagctcgac atcgacacgc
    10141 ggaccgacat ctattcgctg ggcgtgctgc tctacgagtt gctctcgggc gagacgccgt
    10201 tcgaccgcca gcggctgcgg tcggccgcgt ttgacgaagt gctgcggatc atccgcgagg
    10261 aagagccgcc gcgtccgagc accaagctca gcagcagcca ctcgctgccg tcgatcgccg
    10321 ccagccggca caccgagccg cgtcagctcg cgtcgctctt gcgcggcgag ctggactgga
    10381 tcgtgatgaa ggccttggag aaggaccgca accgccggta cgagacggcc aacggactgg
    10441 cgatggactt gctgcggtac ctccacgacg agccggtgct ggcctgcccg ccttctatcg
    10501 gctaccgcgt gggaaaattc gcccgccgca atcggcggtc gctggtgacg gcggcactgt
    10561 tcggtgtgct gcttttgggg gccatcggcg ctatcgcgtc gatcgccggt tgggcggccc
    10621 gcgaccggac ggcccagcgg gcggccgccg cgcagcggat cgccctcgac cgggcggagc
    10681 ggcggatcgt gctggagcgc gagatcgaag cggccctggg cgaggtcgcg ctggagtata
    10741 gccacgaccg cctggcccac gccgccagcg gtcagaagcg ggccgccagc ctgtgggcca
    10801 gcggcgcaag cgaccccgcg ctaaagcagc ggatcgagca gtggcgagtc gaccttcaac
    10861 tcgcgcagcg gctggacgaa attcgcctgg agcaggccgc cgtgaaggac gggcactatg
    10921 cccgcgccgc cgccgatccg gtctattcgg aggcattcca ggagtacggc ctcgatttcg
    10981 acaagctggc tgatgccgcg ctcgctagtc gcgttcagtc gtcggcgatt cgcgaccggc
    11041 tgattccggc ccttgacgat tggttggaga cccgcctgat cgggcaacta tcgggagaca
    11101 agctggtggc cgtcgccgcc gcagccgacg gcgatccctg gcgcaatcgc ctgcggcagg
    11161 ccagcttgaa gagcgatccg aaggagctgc tggcattggc tcaagacgcg gacgtgctcg
    11221 aacacccccc gacgagcttg ctcctgctgg cttcactgct ccgcggcagc gggcaattgc
    11281 aggcggccat cgccttgctg gagcaggccc agcagcagta cccggtcgat ttctggacca
    11341 accagaactt ggggttgtac ctgatgcagc tgccgatgcc gccggccgaa aaggcgatcg
    11401 gctactaccg cgcggcggtc accctgcggc cgacaagccc cggcgtgcgg gtcaacctgg
    11461 gcaacgcgct gcggcggctg aaccggccgg cggaggcgga gcgggaattt cgcacagcga
    11521 tcgagctcaa gcccgactat gccgaggcgc acctgaacct gggaaccgcg ctgcacgact
    11581 tgggattgct cgatgaggcc gagcgagagt ttctgatagc cctcgaaaac aggccaaatt
    11641 atgccaaggc cctcgccaac ctgggcaaca tctaccagat cgagggccgg cttatcgaag
    11701 cggaattctg ttgccgcttg gccgtggcga ttgatcccga cgacctgttt gcccagaaga
    11761 catgcggcga cgtgctgctg aagctagacc gctatgccga gtcggaggcc gcctatcgcg
    11821 aagtcgtccg gctggacccg cgggccggcc gtccccatca agacctggga accgtgttgc
    11881 ggctgcaggg gaagctgaac gacgcggcag cggagtttca gttggcgttg cggctggatc
    11941 cgcggctggg gcagagccag tc
//
LOCUS       DASRJB010000273         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3461224, whole genome shotgun sequence.
ACCESSION   DASRJB010000273 DASRJB010000000
VERSION     DASRJB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461224"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..625)
                     /locus_tag="VFB80_07345"
     CDS             complement(233..625)
                     /locus_tag="VFB80_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33616.1"
                     /translation="MNTLLVAQLDFVRNLDGLSGPQAGWQTLLIVAGAAVSFALGVWL
                     LTRWFGPRERRSLNSPAQLLSELAAAHGLKYAERKLLTRMARHLKLAQPALLFVEPAL
                     WSAGKLGPTWDRARPELDALHKQLFSPA"
     gene            complement(622..2340)
                     /locus_tag="VFB80_07350"
     CDS             complement(622..2340)
                     /locus_tag="VFB80_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN33617.1"
                     /translation="MSCGLTTTFELLGEAENDAATGVLLAALDASQREIRDQALTALL
                     KRHSGTAELNILGRWNDLSLRWKTQVAERVGWLSGAIRKAVLARDARLYECGCSAAVF
                     TRDYDLIPVFVQAAVDRANPYAVRAAAATLELAELLSEELAAPRDYRIRRDPQLQRQH
                     VLAGLERAAAQFDEHGRRELLEAYVLLASREDAVLKRILQSPTDRSFLPLVEVLTNTS
                     RPGAQRLLLSYLDDPHAPLPAIHIIGRRSDVSFLRQLARKISAEPTPVIQANLKRIES
                     LPWITGNLGVLDALSETEQPGAVHLAGSSSAPRHHALEAIAHVLRQGHVPGRRAAAKV
                     LGEFRGPEASELALRSLTDSDPLVRAACAAQLRQRNVPGAINRLLQLLDSPHQAEREA
                     AQAGLSEFRFERFAANFDNLSPEARRSAGPIVRRVDPQAIDQIRAELEAPTRGRRKRA
                     LEMSVALSAVLELQPLVAALLKDEDQYLRIEAIRALAPIDTPAVRQLLRDALLDPQPL
                     VQQAAEAALAGAETVPSPNAEQKETVKLTGRPPTQSRKTLPPGSVPMSSPTETWSSSQ
                     AAALRR"
     gene            complement(2448..3662)
                     /locus_tag="VFB80_07355"
     CDS             complement(2448..3662)
                     /locus_tag="VFB80_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN33618.1"
                     /translation="MSSRKPPLRPEPRTQSPQPNLSRAQQLVLELMAIPGKSGEEAAV
                     AECIRGKLLAVGAPAEAIVSDTAHKHTLIKGNTGNLIFKLPGTLKGPRRMLTAHMDTV
                     PICVGTQPKLDGEFVRSANPQTGLGADNRAGCGAILSAALEILENNLPHPPLTFCWFI
                     QEEIGLYGARCVQKSLLGKPAMCFNWDGGACEKITIGATGGYRMAIDVEGLASHAGVC
                     PERGVSAIAIASLAIADLTQNGWHGLIEKGKHRGTSNVGYIHGGEATNVVTDRVTLKA
                     EARSHNPKFRGQIIKAIEKAFTDAARQVKSSEGKRGSVLIEGRLDYEAFLIDPNSQCV
                     QLAETAVSAVGRQPQRAVSNGGLDANWLNAHGIPTVTLGCGQMNVHTISEMLDLAAYH
                     DACRIALRLATG"
     gene            3744..4790
                     /gene="rlmN"
                     /locus_tag="VFB80_07360"
     CDS             3744..4790
                     /gene="rlmN"
                     /locus_tag="VFB80_07360"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZN33619.1"
                     /translation="MRHLLQPESWDSLKSGLAERSQPAFRAAQIRKWVFEGRAESFEA
                     MSDLPKSLRADLAVEFQLWTSKVVRHLTSDDGTEKLLLELSDGGKIECVLLREDDRRS
                     ICISTQVGCAMGCVFCASGLDGVDRNLTSGEIVEQMLLLQRLLPEGERLSHIVVMGMG
                     EPLANLDSLLPALDEASDETGLGISHRRITISTVGLPKQIHRLCELNTRYHLAVSLHA
                     PSDELRNQIVPVNKNIGLADILAAADRYFEVSGRRLTFEYVLLAGLNDQPQHARELAA
                     RLRGRTALLNVIPYNPVAGLPYVTPSPGAINRFRDILIDAGINVKFRQRKGDAINAAC
                     GQLRRSQPPLVTIG"
BASE COUNT          770 a         1591 c         1737 g          821 t
ORIGIN      
        1 cgcgccagct gcgccggcga cgcctgctcc ggcgacgcct gctccggctc cgaaggctcc
       61 ggcggccgat ccgtttggag cgcccgcgcc gtaagcttcg gcacgacagc ttcctgccgc
      121 gccggcgagc gggaagcgtg agcttcccga taggcggcgt tcgatggcgg cattagcgcc
      181 aacgggaggc tgacgcctcc cgctcgccag tttgcgttac aagggcagat ccctaagcgg
      241 gcgaaaacag ctgcttgtgc agcgcgtcca gttccggacg cgcccggtcc cacgtcggac
      301 cgagcttccc cgcgctccac agcgcgggct cgacaaacag caaggcgggc tgcgccagct
      361 tgagatggcg ggccattcgc gtcagcaact tccgctcggc gtatttcagt ccgtgggccg
      421 cggcgagctc cgagagcaac tgggccggcg agttgagcga gcgccgctcg cgggggccga
      481 accaccgcgt cagcagccag acgcccaagg cgaacgaaac ggcggctccc gcgacgatca
      541 gcagagtctg ccagcccgcc tgcggcccgc tcaggccgtc caggtttcgg acgaaatcga
      601 gctgcgcgac caggagcgtg ttcatcgccg aagtgccgct gcttgagagc tggaccaggt
      661 ctcggtgggg ctggacatgg gcacgctgcc gggcggcagg gtcttgcgcg actgggtcgg
      721 cggccggccg gtcagcttga cggtctcctt ttgctccgca ttcggcgaag gcaccgtctc
      781 ggcaccggcc agggcagcct cggcggcctg ttggaccagc ggctgcgggt cgagaagcgc
      841 gtcgcgcagc agctgccgga cggccggcgt gtcgatcgga gccagggccc gaatcgcctc
      901 gattcgcaga tactggtctt cgtccttgag cagtgccgcc acgagcggct gcagctccag
      961 gacggcggag agcgcgaccg acatttccag ggctcgcttc cggcgcccgc gggtgggggc
     1021 ttccagctcg gcgcggatct ggtcgatcgc ctgcggatcg acgcggcgga cgatcggccc
     1081 ggccgaccgg cgggcctcgg gcgagagatt gtcgaagttg gccgcgaacc gctcaaagcg
     1141 gaactcggac agccctgcct gcgccgcctc gcgctccgcc tggtgcgggc tgtcgagcag
     1201 ctggagcagc cgattgatcg cccccggcac gtttcgctgg cggagctggg ccgcacaggc
     1261 cgcccgcacc agcgggtcgg aatcggtgag gctgcggagc gccagctcgc tagcttcggg
     1321 gccgcggaat tcgcccagga cctttgccgc cgcgcgacgg cccggcacat ggccctgccg
     1381 cagaacgtgc gcgatggcct cgagcgcgtg gtgccgcggc gcggacgagc tacccgccag
     1441 gtgcaccgct cccggctgct cggtttcgct caaggcgtcg agcacgccca ggttgccggt
     1501 gatccagggg agcgattcga tccgcttcag gttggcctgg atgactggag tcggttcggc
     1561 gctgatcttg cgggcgagct gccgcaggaa cgaaacgtcg ctccgccgcc cgatgatgtg
     1621 gatcgccggc agcggagcgt gtgggtcgtc gagatacgaa agcaagagcc gctgcgcccc
     1681 cggccgcgaa gtatttgtaa gcacctccac cagcggcaga aagctgcggt cggtgggcga
     1741 ctggaggatc cgcttgagca cggcgtcctc gcggctggcc agtagcacgt aggcttccag
     1801 cagctcgcgg cggccgtgct cgtcgaattg ggccgcggcc cgctcgaggc ctgccagtac
     1861 gtgctggcgc tggagctgcg gatcgcggcg aatgcggtag tcgcgcggtg cggcgagctc
     1921 ttccgagagc agctcggcca actccagcgt cgccgccgcc gcccggacgg catacgggtt
     1981 cgcgcgatcg acggcggcct gcacgaatac ggggatgagg tcgtagtcgc gggtgaagac
     2041 cgcggccgaa cagccgcatt cgtagagccg ggcatcgcgg gccagcaccg ccttgcggat
     2101 cgcacccgag agccagccga cgcgttcggc gacttgagtc ttccagcgga gcgagagatc
     2161 gttccagcgg ccgagaatgt tcagctcggc ggtgccgctg tgccgcttca gaagggcggt
     2221 gagcgcctgg tcgcgaatct cgcgctgcga ggcgtcgagc gcggccaaga gcacccccgt
     2281 cgcggcgtcg ttttccgcct cgcccagcaa ctcgaaggtg gtggtcaggc cgcagctcat
     2341 caggcaatgc tccacagaaa ctctatgtca cagaccgaga ctccgccggc agaatccgta
     2401 gctgtggcgc aagtctatat agagtaacgg cttgtggagg cgattcccta gccagtcgcc
     2461 aggcgcaggg caatccggca ggcatcgtgg taggccgcta aatccagcat ttcgctgatc
     2521 gtatggacgt tcatctggcc gcagccgagc gtgacggtcg gaatgccgtg ggcgttgagc
     2581 cagttagcgt cgaggccgcc gttgctgacg gcccgctgcg gctggcggcc gacggcggac
     2641 accgcggttt cggcgagctg cacgcactgg ctgttgggat cgatcaggaa cgcctcatag
     2701 tcgagccgcc cttcgatgag cacgctgccg cgtttgcctt cggagctttt gacctgccgg
     2761 gcggcatcgg tgaaggcctt ctcgatcgcc ttgatgatct gcccgcggaa cttgggattg
     2821 tggctgcggg cctcggcttt gagcgtcacg cggtcggtga ccacgtttgt ggcctcgccg
     2881 ccgtggatgt agccgacgtt gctggtgccg cggtgcttgc ctttttcgat cagcccgtgc
     2941 cagccgttct gcgtcaggtc ggcgatggcc agcgaggcaa tggcgatggc gctgacgccc
     3001 cgctcggggc agacgccagc gtggctggcg aggccttcga cgtcgatcgc catgcggtag
     3061 ccgccggtcg cgccgatggt gatcttctcg caggcgccgc cgtcccagtt gaagcacatg
     3121 gccggcttgc ccagcaggct cttttgcacg caccgcgcac cgtacaggcc gatctcttcc
     3181 tgaatgaacc agcagaacgt cagcggcgga tgcggcaggt tgttttccag gatttccaga
     3241 gcggcggaga ggatcgcacc gcagccggcg cggttgtcgg cgcccaggcc cgtttgcgga
     3301 ttggccgagc ggacgaattc gccgtccagc ttgggctgtg tgccgacgca gatcggcacc
     3361 gtgtccatat gcgccgtgag catccgccgc gggcccttga gcgtccctgg cagcttgaag
     3421 atgaggttgc cggtattgcc cttgatgagc gtgtgcttgt gggccgtgtc gctgacgatt
     3481 gcctccgccg gcgctccgac cgccaacagt ttgccgcgga tgcactcggc caccgccgcc
     3541 tcttcgccgc tcttgcccgg gatagccatc agttccagga cgagctgctg ggcgcgggag
     3601 aggttgggct gcgggctctg ggtccggggt tcgggacgga gaggcggttt gcggctggac
     3661 attgcgcggg cggcagactt ggggaggttg aacgtggctg tatgatgata caggagggca
     3721 ggccaaaacg cctgccccac gaaatgcggc atttgcttca accagagtcg tgggattcgc
     3781 tgaagagcgg cctcgccgag cgctctcagc cggcgtttcg cgctgcgcaa attcgcaagt
     3841 gggtgttcga ggggcgggcc gagagctttg aggcgatgag cgatctgccc aagtcgctgc
     3901 gggccgatct ggcggtcgag tttcagctgt ggacgagcaa ggtcgtgcgg catttgacct
     3961 ccgacgacgg cacggagaag ctgctgctgg agctgagcga cggcggcaag atcgagtgcg
     4021 tcctattgcg cgaggacgat cgccgcagca tctgcatcag cacgcaggtc ggctgtgcga
     4081 tgggctgcgt cttctgcgcc agcggcctgg acggcgtcga tcgcaacctg accagcggcg
     4141 aaatcgtcga gcagatgctg ctcctgcaac gcttgctgcc ggagggcgag cggctgagcc
     4201 acatcgtcgt gatgggtatg ggcgagccgc tggcgaatct ggacagcctg ttgccggcgc
     4261 tcgacgaagc cagcgacgaa acgggtctgg gcatcagcca ccgccggatc acgatcagca
     4321 ccgtcggcct gccgaagcag atccaccggc tgtgcgagct caatacacgc taccatctgg
     4381 cggtatcgct gcacgcgccg agcgacgaac tgcggaacca gatcgttccc gtcaacaaga
     4441 acatcggcct ggcggacatt ctcgcggctg ccgatcgcta cttcgaagtc tccggccgcc
     4501 ggctcacctt cgagtacgtg ctgctggccg gcctcaacga tcagccgcag cacgctcgcg
     4561 agttggccgc gcgcttgcga ggccgcacgg cgctgctgaa cgtgattccc tacaatccgg
     4621 tcgccgggct gccctatgtc acgccgtcgc ccggtgcgat caatcgcttt cgcgacatcc
     4681 tgatcgacgc gggaatcaac gtgaaattcc gccagcgcaa aggcgacgcg atcaatgccg
     4741 cttgcggcca gctgcgccgc agtcagccgc cgctggtgac catcggctaa gaggactggt
     4801 ccaacgaagg aagaactcac cgtccgcccg actccgggat cggccaggcc cagccggcgg
     4861 tgaagccgcc gtcgatgtag agtgtttggc ccgtgatgaa agcagcggcg ggggaggcg
//
LOCUS       DASRJB010000274         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1206565, whole genome shotgun sequence.
ACCESSION   DASRJB010000274 DASRJB010000000
VERSION     DASRJB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206565"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /locus_tag="VFB80_07365"
     CDS             <1..2163
                     /locus_tag="VFB80_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN33620.1"
                     /translation="LQEFAIRWAYLIRNRTHWNKTKWAAMRSQLQRQVKSVGFRQADF
                     RKLGECQLIEVEIPFRREEEGWEARVLPWEFVLTNLAEGRSDGLPPIVVRRLKVRGRR
                     RVSQPPAAAKLAYVQSAPGALAAEYDFSKEKKLVVNSLRHTHWLQKTLEVIDPTLEEL
                     RSALQEFCPTVVHFTGVDNHQGAELLAEAHTPLLDGVYLKCEAPPGCVPIEAKVFAPA
                     IAGESCRPALVGLNMYHSAPRIAPLAIAAGAGAVVAFQDAVDDTLAELFFADFYRAWS
                     KSQDLLAAFRGTLHNLMRSSGQTLAGTGVVLWSAASLLLPTTAPPPDEENYSVPARLA
                     ELDPQQPLEQQIQVDVKVRDNINYSLLHNGRSLFQQLYVVNRTGKRLDNLQVRVRLFV
                     AGDTDEQVTRLNVAGPGVDVGRLVSVPLISRQLRCYRESVLTSLKVTVEWEGRVIKDE
                     NHTVRVLPLNEWCEYEGESQEPQGQWLPSFVQPLDPAVSQILSKAERHLRTITGDPYA
                     GFDGYQQVDGRREFKVVDQQVEAIWAALLHDYSIAYVNPPPVYTPGSQRIRSPSEVVE
                     QGRGTCLDLTVLVASCLEHVEIYPAVVLLTGHAFPAYWRSPALHEEFWGGGQAAIEER
                     IDVDVDHAAVSARGRLDDQRGDYLWMTRPSGYSRLTKAVARGHLVPLESVWLTARESF
                     DEACEAGGENLIDRSEFECLLDVRRAREENVLPLPERT"
     gene            2163..>2343
                     /locus_tag="VFB80_07370"
     CDS             2163..>2343
                     /locus_tag="VFB80_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33621.1"
                     /translation="MARKRPAETRLSEAELAALREDLALAGKDVGVAPDFATPKKSDG
                     LRRLSTKELRARRQSR"
BASE COUNT          396 a          741 c          822 g          384 t
ORIGIN      
        1 ctgcaggagt tcgcgatccg ctgggcctac ttgatccgca accgcacgca ttggaacaag
       61 acgaagtggg ccgcgatgcg cagccagctg cagcggcagg tgaagtcggt cggatttcgc
      121 caggccgact ttcggaaact cggcgaatgc caactgattg aggtcgagat cccgtttcgc
      181 agggaagagg agggctggga ggcccgcgtc ttgccgtggg aattcgtgct gaccaacctg
      241 gcagaaggcc ggtccgacgg cctgccgccg attgtcgtcc gccggctgaa ggttcgcggc
      301 cgccgccgcg tgtcgcagcc gccggcagcg gccaagctgg cttatgtgca atccgcgccc
      361 ggagccctgg cagcggaata tgatttctcc aaagagaaga agctggtcgt caacagcctg
      421 cggcacacac actggctgca gaagaccctg gaggtaatcg atcccacgct ggaggagctg
      481 cggtcggcac tgcaggagtt ttgccccacg gtcgtgcact tcaccggcgt cgacaatcac
      541 cagggcgcgg agctgctggc ggaggcgcac acgccgctgc tcgatggcgt gtacctgaaa
      601 tgcgaggcgc cgccggggtg cgtccccatc gaggccaagg tgtttgcccc ggcgatcgcc
      661 ggcgaatcgt gccggcccgc gctggtgggg ctgaacatgt accactccgc gccgcggatc
      721 gcgccgctgg cgatcgccgc gggcgcgggc gccgtggtgg cgttccagga cgccgtggac
      781 gacacgctgg ccgagctgtt tttcgccgac ttctatcgag cctggagcaa gtcgcaggac
      841 ttgctggccg cgtttcgcgg cacgctccac aacctgatgc ggagcagcgg gcagaccctg
      901 gccggcacgg gcgtcgtgct gtggagcgcc gcgtcgctgc tgctgcccac gacggcgccg
      961 ccgccggatg aagagaacta ctccgttccc gccaggctcg ccgagctcga cccgcagcaa
     1021 cccctggaac agcagattca ggtcgatgtg aaggtacggg acaacatcaa ctactcgctg
     1081 ctgcacaatg gccgcagcct gttccagcag ttgtacgtcg tcaaccgcac cggcaagcgg
     1141 ctcgacaacc tgcaggtccg cgtccgcctg ttcgtggccg gcgacaccga cgaacaagtg
     1201 acgcggctca atgtggctgg gcccggcgtg gacgtggggc ggctcgtttc cgtgccgctc
     1261 atcagccggc agctgcgctg ctatcgcgaa agcgtattga ccagcttgaa ggtgacggtg
     1321 gaatgggaag gccgagtcat caaggacgag aaccatacgg tccgcgtgct gccgctcaac
     1381 gaatggtgcg agtacgaggg cgagagccag gagccgcagg ggcagtggct gccctcgttt
     1441 gtgcagccgc tggatccggc ggtttcgcag attctctcca aggcggagcg gcacctgcgg
     1501 acgatcaccg gcgaccccta tgccggcttc gacggctatc agcaggtgga cgggcggcgg
     1561 gaattcaagg tcgtggacca gcaggtagag gcgatctggg cggccctgct gcacgactat
     1621 tcgattgcct atgtgaatcc gccgccggtc tatacgcccg gctcgcagcg gatccgctcg
     1681 cccagcgaag ttgtggagca gggccgcggc acgtgcctcg acttgacggt gctggtggcc
     1741 tcgtgcctgg agcacgtcga aatctatccc gcggtcgtgt tgctcacggg gcacgccttt
     1801 ccggcctatt ggcgctcgcc cgcgctgcac gaggagtttt ggggcggcgg ccaggcggcc
     1861 atcgaggagc ggatcgacgt ggacgtcgat catgcagccg tttcggcccg cggacgcctc
     1921 gacgaccagc gcggcgacta tttgtggatg acgcggccca gcggctattc gcggctgacc
     1981 aaggccgtgg cgcgggggca cctggtgccg ctggaatcgg tgtggctgac ggcccgcgag
     2041 tcgttcgacg aggcgtgcga agcgggcggc gagaacctga tcgaccgcag tgagtttgaa
     2101 tgcctgctcg acgttcgccg ggcccgcgaa gaaaacgtgc tgcccttgcc ggagaggacc
     2161 tgatggctcg caaacggcct gccgagacgc gtctgagcga agccgagctg gcggcccttc
     2221 gcgaagacct ggccctggcg ggcaaagacg tcggcgtcgc gccggatttt gctacgccca
     2281 aaaaatccga cggactgcgg cggctttcga cgaaggagct tcgcgcccgt cgccagagcc
     2341 gtg
//
LOCUS       DASRJB010000275        18327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4027806, whole genome shotgun sequence.
ACCESSION   DASRJB010000275 DASRJB010000000
VERSION     DASRJB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 18327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18327
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4027806"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFB80_07375"
     CDS             complement(<1..423)
                     /locus_tag="VFB80_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZN33622.1"
                     /translation="MSEATIPLVDSHRALSLFGARDQHLRTIRETLGVAITHRDGEIR
                     VSGEETAVARATAALNQLSSRLDQQGELTGDHVADVLGHVTGKREAAPPQTPVDVIHA
                     ARKVSPRTQGQARYIQSIRDHDITFATGPAGTGKTYLAV"
     gene            complement(613..1887)
                     /gene="hemA"
                     /locus_tag="VFB80_07380"
     CDS             complement(613..1887)
                     /gene="hemA"
                     /locus_tag="VFB80_07380"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZN33623.1"
                     /translation="MKLHMVGCSHHNAAVAVRERLAFSRDQAAEALTRFRTRYPEAEA
                     VLLSTCNRVELYFASETPDACPSHHDAVNFLAEFHGLSPTELFNELFEHTGEDFVRHL
                     FTVAASLDSMVIGETQILSQVKQAYELANSVAATGTITNSAFQAALRVAKRVATETAI
                     QQKRVSIPSVAVAEFAKGIFERFDDKHVLVIGAGEMGEEALRYLIDEGATQISLVNRS
                     LARAEDLAGRLAGQPRPWSDLHALLVEADLVVSATGSQEPILTAADFRPIVGERFQRT
                     LLILDLAIPRDFDPAINDLTGVFLYSIDDLKAACDRNRQEREKEWPKAQKLIDEETAR
                     FMADLHHRATAPTIRRLKARADEVKADELARLLNKLGNVDERSRAEILSAFDRLVNKL
                     LHPPLESLRDEAERGPPHGLLDALKRLFKLKD"
     gene            complement(1884..2834)
                     /locus_tag="VFB80_07385"
     CDS             complement(1884..2834)
                     /locus_tag="VFB80_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HZN33624.1"
                     /translation="MGRKQYGMLSGIHIFCFFATCALVVLCLEISRLFFRMPVRLIVM
                     LGIAAVGFIAQTLYLGHRAWTSSLPLSTWHDWFLVAAWILVAAYLGLTMSRPTTMVGV
                     FLLPVVIALMLVAAAVPRDVPFARDRALQAWELAHGLLLLAGTVPVSLGFVAGVMYLV
                     QSWRLKHHVSPQAGLKLPSLEWLQAVNRQSLIFSSFFIALGLVAGIVLNTIKAREYGA
                     GLPWTDSVVISSAILLVWLLAATAFEWLYKPAQQGRKVAYLTVASFIFLAFVMAMLVA
                     GGSKHAKGGAGLQPALPSPRSSVAHAAGSKPAPLMTEARA"
     gene            complement(3036..4118)
                     /locus_tag="VFB80_07390"
     CDS             complement(3036..4118)
                     /locus_tag="VFB80_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN33625.1"
                     /translation="MSPYRPPPAAETGPPIPGLIGTSPAMEAVYRLTRKVAKTNASVL
                     LLGETGSGKEMIATSIHRLSQRASGPFVRVNCGALSESLLESELFGHVRGAFTGAINN
                     RTGRFEAAHTGTIFLDEINSTSLFLQVKLLRVLQEREFERVGDTTTNRVDVRVIAASN
                     RDLYAEVDAERFREDLYWRLNVVPIVIPPLRMRREDIPALVAHFLNYYSEKNDKNVVH
                     IQAEAIEALQEYHWPGNVRELQNYVERAVAMADGDELTVELLPDAVLGRAERRAGRIR
                     GVDLETLVFEVVQQGVTTAPSDETNLHEKIVNRVERELIVQVMQSCNNVQTKAATRLG
                     INRNTLRSKLQQYNLEEANGEDTKTG"
     gene            complement(4240..6564)
                     /locus_tag="VFB80_07395"
     CDS             complement(4240..6564)
                     /locus_tag="VFB80_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33626.1"
                     /translation="MTHRLWISTFGILAASAAAVVLVGALAPAQDPFGAPMPKAGAPQ
                     AGGAPPAADPFGGAAQPGAARPAAAAPADPSKQDPLAIQILRDSNPRAPAELMQAAQT
                     ALKFGRPDETRRYLGLLVAAKPADDALAPLAARFGEFLFQLASTKDVQPEGKQVADGV
                     LAAAQRVAQNIDRINALIPQLSAESLGERQDALGKLATGGSTIVTPLLRVLADPAREK
                     EHRYIRAALVHLAIDTEAPLLGALETPNENLKTQIIAVLGRMRSHRATMHLVRPAVDP
                     QAPSELRATAAGALQMILGASPDRYEAEKYLAQQTARLLEGELPYRPDPADRVELWSW
                     DEAQRQVTARKLPKRDAALLLATRVASDLYALKAGDNAALRLMLLTNLDLAKTLGGLD
                     RPLPVDAASAGAAAMAAGPTVVNQVLADALKHGRVAAAIAAAEVLEKLGNASVLTPHD
                     GISPLAEALKHPDRRVRLAAALAIARLAPAASFPGAGQLAPALAWFAATSGEDMVLVA
                     HPRGEDAQSLVGFMNELGRAGQAAYIGRYLMEEAVVNPDVEFILISDAIDLPPVQELV
                     QWLRRDFRTAQIPVGVMARSELLDRTRFAFVDDPLTTVFPRIYSTNVAAVEVARLEEI
                     AGRNRVSRDERIDQARAALAALATLAKTPDNFARHELLRQEPLVIRALANPALSVAAA
                     NVLGLFGSPRAQTALVDFASQNTRPLADRQAAAAAFAAAVKARGLLLTQAQLAAQYAR
                     YNASETLDKDTQAVQGSLLDAIEAPAIARGDLKK"
     gene            complement(6575..7960)
                     /gene="argH"
                     /locus_tag="VFB80_07400"
     CDS             complement(6575..7960)
                     /gene="argH"
                     /locus_tag="VFB80_07400"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZN33627.1"
                     /translation="MATPSRSGVFSKATDRRVERFTESVSFDHRLFAHDIAGSIAHAQ
                     MLAAVGLINEQECRQICDTLQQIRGEIEAGKFPLRTELEDIHMHIEQALIERLGDVGR
                     KLHTGRSRNDQVATDLRMWIRDALDRVDSKILELQRAFIGRCDDDFDVILPGYTHLQR
                     AQPVLAPHYWLAYCEKLQRDRLRVMDCRRRVNVSSLGAAALAGSSLPIDRQDVARRLG
                     FEAVAANSLDISSDRDFVLETAFVLTLIAEHLSTWAEEWILWSTAEFNFLKLPQEFCT
                     GSSIMPQKVNPDVLELTRGKTARVIGNLQSLLVLVKGLPLAYNRDLQEDKERIFDSFD
                     TVELCLELAAPLVAGTALHRQAIAEQLDRGHLDATTLMEWLILRGMPQRTAHHLVGQL
                     VGTALARGCRLADLAIADFRAADPSLDESVYGVLGASRAVNAFRSYGSTAPGEVRKQI
                     DRWKQLVGHAS"
     gene            8536..10923
                     /locus_tag="VFB80_07405"
     CDS             8536..10923
                     /locus_tag="VFB80_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN33628.1"
                     /translation="MYRFARWTIWISLILLCLPLAAQDAPTNPLDEPLPKLNIAGQLG
                     IGDAGGEKVTLTGSFTIEKGSRRGFLTITAQIEPEWHIYSITQPDGATQRTRLEPAKS
                     ADYKLLGPFQPDIAPKIKKYPYWKVPVEEHEGQVKWTAPIEIAAGVKPESLTIQVKAS
                     GQVCKDNCQDFGSDISAALAGYSESSASAGEYQPPSGDAQLALKGHLEPAAAAPGSKA
                     RLVITAIPNEGWHVYPYSPQDPNQVGVNKPTLIHLNPLTPGWTRSPVVESTTPKGGHH
                     EEPVSWTIELSIPGDSPAGESVVSGAIGLQTCNPGRCLPPYAVRFRAALPVKAKAEEG
                     RIPLEFIPVDSADKSATIKGYNDVAKLAAEHPAPTGKVNFATLLPMIGFGLLGGLILN
                     LMPCVLPVIGLKVLSFIQQGGQSRGSIFALNVWFALGLLSVFVALATLAAFGSLIPQL
                     GQNLSWGQQFTFTSFKVAMVVVVFAFALSFLGVWEVPIPGFAQSQASGKLQQQEGPAG
                     AFFKGIFTTLLATPCSGPYLGLVFGYTLAQPPLATYLIFTSVGLGMAAPYLLIGAFPG
                     LVRWLPKPGEWMETFKQVMGFVLLGTVVYLFATISADYFIPTLALVIGVWFACWLIGR
                     VPIYEDTGKQLRQWAIGIAAAAVTGWLAFTYLGPVKHLYEWQPYTPEAIAKLQTEGKT
                     VMVDFTADWCLTCQANFRLAINTHRVKEVVEKNQVVPLLADWTDKNDTIKQKLADLNS
                     NSIPVLAIYPAGKPGEVIILRDALLERQVIRALEEAGPSQPAAAATAMVENPTGG"
     gene            complement(10937..11074)
                     /locus_tag="VFB80_07410"
     CDS             complement(10937..11074)
                     /locus_tag="VFB80_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33629.1"
                     /translation="MGIRPRTKFTMQLKNRRCPKCGAKLNNQVKRCKRCAAVAGKPRK
                     R"
     gene            complement(11169..11987)
                     /locus_tag="VFB80_07415"
     CDS             complement(11169..11987)
                     /locus_tag="VFB80_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33630.1"
                     /translation="MNISPLERRVLAIGTAVVLHAAILGLAAAVHAAAQAAGRVPALP
                     QGPPLLLAAVALVMAHCSLGAIWWARSNWPSYAKTLVAVLATAALWALLIGVLESTDL
                     AETAAAAWAASLATQVVLVGLGAALLELLVAPPGVAERSRFTILILLLWTAVVGVLLG
                     AGRRLAQALGWNLADVLRWQWFYQLQMIGVSNAALAVSLLVAVRLRREWTVRLLVAGL
                     ALFFVGLVPPLAMTAMFKGNLGASFADVAWLLLVQGLFVLATLAPLEAAKELPK"
     gene            complement(11984..12478)
                     /locus_tag="VFB80_07420"
     CDS             complement(11984..12478)
                     /locus_tag="VFB80_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZN33631.1"
                     /translation="MSIATAVRSVARLLKRLDRKVAFAESCTGGLVSGSLTKIPGISN
                     YHCGGVVVYRNQTKQAYLGIAAELLEKPGPVSREVAEQMAQRVLHMTPEADLAASVTG
                     HLGPNAPPKLDGLVFAAVAWRSPQRVRHRRLQCGRRDSRATRQRWVVEQVLLLLAAEL
                     EAAR"
     gene            12542..13546
                     /locus_tag="VFB80_07425"
     CDS             12542..13546
                     /locus_tag="VFB80_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN33632.1"
                     /translation="MNPRQQLEPPVAGLVDPHLFGTLCGLFSALIYTCANSFLRAVHH
                     CDPVWVSTIRAVPTVAIMAPVLALLASRGRPVVPRPRYLLLIAVGGLCGQLGGNICFQ
                     SALHIIGVALAVPLCMGGMIVSAAILGRVFLAEPLTPRVLLALAILLAAIAVLAIGAG
                     EAGRAMQSSADSLPAPAPPWRLAAGVAAGCGSGVFYTLLNTILRYCVTRGAPLPTSLF
                     IVSTVGVIGLGSIAWLRIGGAGMRATLPADLAIMLAAGLCNTVAFLALTKSLQLNSIV
                     YVNALNATQATLAAIAGVVIFREKLSPWLVVGIGLTIVGLMALARAHRALRTPGESAS
                     "
     gene            complement(13575..13832)
                     /locus_tag="VFB80_07430"
     CDS             complement(13575..13832)
                     /locus_tag="VFB80_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33633.1"
                     /translation="MGAPSRHQFTLRTILLVVAVVPPLIGGLTGGLGPHVKNGLFLAL
                     GIPALALALLGLTFVLTWLVFVLPIAMLIPEPDDSDSSRCE"
     gene            13959..15857
                     /locus_tag="VFB80_07435"
     CDS             13959..15857
                     /locus_tag="VFB80_07435"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZN33634.1"
                     /translation="MPAETVTPPSVKPRRAAATQLPTFVFESSDDLARHVAQLVAGVI
                     RERNAHGQMAVLGLPTGSSPMGVYRELARMHREEGLDFSQVAAFVLNEFYGVAPDQLQ
                     SHHRWIREHLLDHINIPPANVHVPCGTTPLAEVENHCRDFESRIEHAGGIDVLLLGIG
                     RNGHIGSNEPFPGVGGRTRLSTLDPVTRRALASDFFGEWNVPTQALTMGLGTIFDARK
                     VLLLAQGEHKAEIIREVAEGPVSPRVPASYLQEHADAAVLVDSAAGSQLTSSQTPWAI
                     SNVQWTDALIKRAILWLCGQTGKALLKLTDEDFRNHNLHQLLRHHGPAPKVAHQVFQQ
                     LMGTIVYHPAGKEPQRVICFSPHPDDDVISMGGTLIRLIEDGHETHIAYMTSGNIAVF
                     DHDAARVADLVTEYNRLFGIDQERSKLVEGTVLESLRRKTPGEPDIEAVQKIKGLIRW
                     GEAKAGALKVGCREEHLHFLDLPFYRTGTIAKKPICDHDVQIIRDLLARVQPQQIYVA
                     GDLSDPHGTHRVCAQAIFRAVLEHEAQHGSRPQVLLYRGAWQEYELFEIEIAVPLSPG
                     DILKKRKAIFMHQSQKDEALFPGSDPREFWQRAEDRNTNTAAQYNQLGLPEYFAMEAF
                     VRWNGVPI"
     gene            15994..16689
                     /locus_tag="VFB80_07440"
     CDS             15994..16689
                     /locus_tag="VFB80_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33635.1"
                     /translation="MAISTAVLLPGLDGTGELFRPFVEAAPRGIETAVVDYPTSEASI
                     EILERRAREKLATRCIVIAESFSGPIGVRLAADARVQALVLCNSFVSSPACSLLRHLT
                     LAPLFAIPIPRFMLRLLLLGRQASPTLVENTRRAIRRVPATVIARRVRQVLQTDERET
                     VRALEKPVLYLRGRSDHLVSERSWRDLQAVRPDAQIARIEGPHMLLQVSPHECWEAIV
                     GFTNGIRGGLTPA"
     gene            16869..17342
                     /locus_tag="VFB80_07445"
     CDS             16869..17342
                     /locus_tag="VFB80_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33636.1"
                     /translation="MEKTGSFYQRAGLGKHTQPASAGLLAWLRQVGASEALLPAFGDS
                     LREDVEEFPLVFLAESELLRVNAPGTGGLCCGIAQGLLVVGASALSGDPVVIDLRDPA
                     GAGGYLNHETMWSAADLRGEIWRAAHSWEQLAVLIDQCKFPSNYHDAKQSGPPGT"
     gene            17436..>18327
                     /locus_tag="VFB80_07450"
     CDS             17436..>18327
                     /locus_tag="VFB80_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33637.1"
                     /translation="MLTLHSSSSRDCSGLARRDFVRAGVLGIGALALPQLLAAKAAAK
                     AAGKEYVRDRSIVLLFLGGGASHIETFNPNIDAPAPFASVTGEVKTKVPGLTLGGTFP
                     QLAKHAQKLAVVRSFSHENGNHPKAIIHVLSGGTDPTGDQKAGQSMGSVYARLRGANH
                     ESSGLPTYCLLTSDEVDGQYRNEKSRIAAGSWPGQLGLAFAPFDPAGGGQLLKNLSLG
                     VSAERLDDRKTLLAKLDTVKRSIDASGNLEGIDRFERQALEMIVRGAGEAFDIRREDR
                     RTLDRYDTTQFRVGKKEFQPS"
BASE COUNT         3216 a         6036 c         5934 g         3141 t
ORIGIN      
        1 cacggccagg taggtcttgc cggtgcccgc tgggccggtc gcgaaggtaa tgtcgtggtc
       61 gcgaatcgac tggatatatc gcgcctgccc ctgcgtgcgg ggggagacct tgcgggccgc
      121 gtgaatcacg tccaccggcg tctgaggcgg ggccgcctcg cgcttgccgg tcacgtggcc
      181 cagcacatcg gccacgtgat cgcccgtcaa ttccccctgc tggtcgaggc ggctgctgag
      241 ctggttgagg gctgctgtcg cccgggctac ggcggtttcc tcgccgctca cccggatctc
      301 gccgtctcga tgcgtaatcg ccacgccgag ggtctcgcgg atggtccgca agtgctggtc
      361 ccgcgcaccg aacagcgaca gcgcgcggtg ggaatcgacc agcggaatgg ttgcctcact
      421 catgttctcc ggcagcacac ccgccgccgc caccagcccc ggaatcgcgg cccctccagg
      481 ccgcccgccc taaagcgaat aggtaattgt aatatatcgg gggcgcgtca ggggcgggta
      541 gcgattgtaa cggctggcga cgcaagtgct tactcagtaa aggcttgcgc gtatgtgcgg
      601 actgccgaat gcctagtcct tcagcttgaa cagccgcttg agggcgtcca aaaggccgtg
      661 gggcgggccc cgctcggctt cgtcgcggag cgattccagc ggagggtgca gcagcttgtt
      721 gaccagccgg tcgaaggcgg acaggatctc cgcgcgcgat cgttcatcaa cattgcccag
      781 tttgttgagc aggcgggcca gttcgtcggc cttcacctcg tcggcccggg ccttcaggcg
      841 gcggatcgtg ggagcggtgg cccggtggtg caggtcggcc atgaagcggg cggtctcttc
      901 gtcgatcagc ttctgggcct tgggccactc cttttcccgc tcctgccggt tgcggtcgca
      961 ggccgctttc agatcgtcga tggaatagag aaacacgccg gtcaggtcgt tgatggccgg
     1021 atcgaaatcg cgggggatcg ccaggtcgag aatcagcagc gtccgctgga accgctcgcc
     1081 tacgatcggc cgaaagtcgg ccgcggtcag gatcggctcc tgcgatccgg tggcgctgac
     1141 gaccaggtcg gcctccacca gcagggcgtg caggtcgctc cagggccgcg gctgcccggc
     1201 cagccggccg gcgaggtcct cggcgcgggc caggctgcga ttgacgagcg aaatctgcgt
     1261 ggctccttca tcgatcaggt accgcagggc ttcctcgccc atctcgcccg cgccgatcac
     1321 caggacgtgc ttgtcgtcaa aacgctcgaa gatacccttg gcgaactcgg cgacggcgac
     1381 gctgggaatg ctgacccgtt tttgctgaat ggcggtttcc gtcgcgacgc gcttggccac
     1441 gcggagcgcc gcctgaaacg ctgagttggt gatggtgccg gtcgcggcga cgctgttggc
     1501 cagctcgtag gcctgtttca cctgcgacag tatctgcgtc tcgccgatca ccatgctgtc
     1561 caggctggcg gcgaccgtga acaggtgccg gacaaagtcc tcgccagtgt gctcgaacaa
     1621 ttcgttgaac agctcggtcg gcgacaggcc gtgaaactcg gccaggaagt tcaccgcatc
     1681 gtggtgcgac gggcaggcgt ccggcgtttc gctggcgaaa tagagctcga cgcggttgca
     1741 ggtcgagagc agcaccgcct ccgcctcggg atagcgcgtg cgaaaccgcg tcagggcctc
     1801 ggccgcctga tcgcggctga acgccagccg ctcccgcacg gccaccgccg cgttgtggtg
     1861 gctgcagccg accatgtgga gcttcatgcc cgggcctccg tcatcagtgg ggcaggtttg
     1921 gaacctgccg cgtgagcaac agacgaccga gggctgggta gggcaggttg caaacctgcc
     1981 ccgccttttg cgtgcttcga tccgcccgcc acgagcatcg ccatcacgaa ggccaggaag
     2041 atgaagctgg cgacggtcag ataggccacc ttgcggcctt gctgggccgg cttatagagc
     2101 cattcgaacg cggtggcggc aagcagccac acgagcagga tggccgagct gatgacgacg
     2161 ctgtcggtcc acggcaggcc ggcgccgtac tcgcgggcct tgatcgtgtt gaggacgatg
     2221 cccgccacga gcccgagcgc gatgaagaac gacgagaaaa tcagcgattg cctgttgacc
     2281 gcctgcagcc attcaaggct cggcagcttc agcccagcct gcggcgacac atggtgcttg
     2341 agccgccagg actgcacgag gtacatcact cccgcgacaa agccaagcga aacggggacg
     2401 gtgcccgcca gcagcaacaa accgtgagca agttcccaag cttgcagcgc gcgatcgcgg
     2461 gcaaagggaa cgtcgcgcgg cactgccgcc gcgaccagca tcagcgcgat gacgaccggc
     2521 agcagaaaca cgccgaccat cgtcgtcggc cgcgacatgg tcagccccag ataggccgcg
     2581 accagaatcc acgccgccac caggaaccag tcgtgccacg tcgaaagcgg cagggagctc
     2641 gtccaggccc gatgtcccaa atacaacgtt tgggcgatga acccaacggc tgcgattccc
     2701 agcatcacga tcagccgcac tggcatgcga aagaacagcc gcgaaatctc caggcacagc
     2761 acgaccagcg cgcacgtcgc gaagaagcag aagatatgta ttcccgagag catgccgtat
     2821 tgcttgcggc ccaacgggcc ggccttcgtc agcccagggc gaagccctgg gttatcgacc
     2881 accacacacc attcagcccc aaacggggcg aaactcggca gcgcccatga cgcccctttg
     2941 gggcggcgac tcagtcggga attcgttacc cagggcggcg ctgggcttgc cctgggctgg
     3001 cgaaggttgc cccgttgggg caagccacta ttggctcatc cggtcttggt gtcctccccg
     3061 ttcgcttcct ccagattata ttgctgcaac ttgctccgta aggtgttgcg gttgatgccc
     3121 aggcgggtgg cggctttggt ctgaacgttg ttgcaggatt gcatgacctg cacgatcagc
     3181 tcgcgctcga cgcggttgac gattttctcg tggaggttgg tctcgtcgga cggggcggtg
     3241 gtaacgccct gctgcacgac ctcgaagacg agcgtttcca gatcgacgcc gcggatgcgg
     3301 cccgcgcggc gctcggcccg gcccaggacc gcgtcgggca agagctccac cgtcagctcg
     3361 tccccgtcgg ccatcgccac ggcccgctcg acatagttct gcagttcgcg gacgttgccc
     3421 ggccagtgat actcctgcag ggcctcgatc gcctcggcct ggatgtggac cacgttcttg
     3481 tcgttcttct cgctgtagta gttgaggaag tgggccacca gggccggaat gtcctcgcgc
     3541 cgcatccgca gcggcggaat gacgatcggc acgacgttca gtcgccaata gaggtcctcg
     3601 cggaaccgct cggcatcgac ctcggcgtac aggtcgcggt tggaagccgc aatcacgcgg
     3661 acatcgacgc ggttcgtcgt ggtgtcgccg acccgctcaa actcccgctc ctggagcacg
     3721 cggagcagct tcacctgcag gaacagcgac gtgctgttga tctcgtcgag gaagatcgtc
     3781 cccgtatgag cagcctcgaa acggccggtg cggttgttga tcgcgccggt gaacgccccg
     3841 cggacgtggc cgaacagttc actctcgagc aggctctcac tgagcgcccc gcagttcact
     3901 cgcacgaacg gcccgctggc ccgctggctc agccggtgaa tcgacgtcgc gatcatctcc
     3961 ttgccgctgc cggtttcgcc cagcaggagg accgaggcgt tggtcttggc gaccttccgc
     4021 gtcagacgat agaccgcctc catcgccggg ctggtgccaa tcagtccggg aatcggcggt
     4081 ccggtttcgg cggcaggcgg tgggcggtag ggcgacatag gcggcatccg tgacgcggat
     4141 ggaacgcggt cagtaaacga accaagcccg cgagcaaatc tcgcgggctt agcagttgct
     4201 caaatgttgg gcagcagtac tacttatcaa gcaggcttcc tatttcttca aatctccccg
     4261 cgcgatcgcc ggtgcttcga tggcgtcgag cagcgagccc tgaaccgctt gggtgtcttt
     4321 gtcgagcgtt tcgctggcgt tgtagcgcgc gtattgcgcg gcgagctgtg cttgggtcag
     4381 cagcagcccg cgggccttga ccgccgccgc aaaagccgcg gctgccgcct gccggtcggc
     4441 cagcggccgc gtattctggc tcgcgaaatc gaccagcgcc gtctgtgctc ggggcgagcc
     4501 aaacagcccc agcacattcg ccgccgcgac gctcaaagcg ggattggcca gcgcgcggat
     4561 cactagcggc tcttgccgca agagctcgtg ccgcgcgaag ttatcgggcg tcttggccag
     4621 cgtcgccaac gccgccaagg ccgcgcgggc ctgatcgatt cgctcgtcgc ggctcacgcg
     4681 gttgcgtccg gcgatctcct ccagcctggc cacttccacc gcagccacat tcgtggaata
     4741 gatccgcgga aagaccgtcg tcagtggatc gtccacaaaa gcgaagcggg tgcggtcgag
     4801 cagttcgctg cgggccatta caccgacggg aatctgcgcc gtgcggaagt cgcggcgcag
     4861 ccattggacc agctcctgca ccggcggcag atcgatggcg tcgctgatca ggatgaactc
     4921 gacgtcggga ttgacgacgg cctcctccat caaatagcgg ccgatgtaag ccgcctgccc
     4981 ggcgcggccg agctcgttca tgaagccgac gagcgactgg gcatcttcgc cgcgcggatg
     5041 ggcgaccaga accatatctt cgccgctcgt cgcggcaaac caggccagcg ccggcgcaag
     5101 ctgccctgct cccgggaacg aagcggccgg cgcgagcctg gcgatcgcca gcgccgccgc
     5161 cagccgcacg cggcggtcgg gatgcttgag ggcttcggcc agcgggctga tgccgtcgtg
     5221 cggcgtcagc acgcttgcgt ttcccagttt ttcgagcact tccgcggcgg caatcgccgc
     5281 cgcaactcgg ccgtgcttca gcgcatcggc caacacctgg ttgacgacgg tcggacccgc
     5341 ggccatggcg gccgcgccgg ccgaagcggc atccacaggc agcggccgat ccaaaccgcc
     5401 cagcgtcttg gccagatcca ggttggtcag cagcatcagc ctcagggccg cgttgtcgcc
     5461 agccttcaag gcgtacaaat cgctggccac gcgcgtcgcc aggagcagcg cggcgtcgcg
     5521 ttttggcagc ttgcgggctg tgacctggcg ctgcgcttcg tcccacgacc agagctcgac
     5581 gcgatcggcc ggatcgggcc gatagggcaa ttcgccttca agcaggcggg ccgtctgctg
     5641 cgccaggtat ttctcggctt cgtagcggtc gggcgaggcg cccaggatca tctgcaaggc
     5701 accggcggcc gtggctcgca gctcggaggg cgcctgcgga tcgaccgcgg gccgaacgag
     5761 gtgcatcgtc gcgcggtgcg agcgcatccg ccccagcacg gcgatgattt gcgtcttcag
     5821 attttcatta ggcgtctcca gcgcgccgag cagcggcgcc tcggtgtcga tggccaggtg
     5881 cacgagtgcc gcacgaatgt agcggtgctc tttctcccgc gcgggatcgg ccagcactcg
     5941 caatagcggc gtcacgatgg tcgagccgcc tgtcgccagc ttgcccaggg cgtcttgccg
     6001 ctcgcccagc gactcggccg acaattgcgg aatcagggcg ttgatccggt cgatgttctg
     6061 ggccactcgc tgcgcggcgg cgagcacgcc gtcggcgacc tgcttgccct cgggttgaac
     6121 atccttggtg ctggccagct ggaacaggaa ttcgccgaac ctggccgcca gcggcgcgag
     6181 cgcgtcgtcg gccggcttag cagccaccag caggcccaaa taacgcctgg tttcgtcggg
     6241 ccgcccgaat ttcagcgccg tctgcgccgc ctgcatcagc tcggcgggcg cccttgggtt
     6301 gctgtcgcga aggatttgaa tcgccagcgg atcctgcttc gacggatcgg ccggcgcagc
     6361 agcggccggc ctggctgcgc caggctgagc agcgccgcca aacggatcgg ccgctggcgg
     6421 agcgccgccg gcttgaggcg cgccggcctt gggcatgggt gcgccaaacg ggtcctgcgc
     6481 cggcgccagc gcgccgacca gcaccacggc cgctgccgag gcggccagta ttccgaaggt
     6541 cgaaatccaa aggcgatggg tcatggtgca gtggtcagga tgcgtgccca acaagttgct
     6601 tccagcggtc aatctgtttg cggacttcgc ccggcgcggt cgagccgtag ctgcggaagg
     6661 cgttcaccgc gcggctcgcg cccagcactc cataaaccga ttcgtccagc gacggatcgg
     6721 ccgcgcgaaa atcggcgatc gccaggtcgg ccagccggca gccgcgggcc agggcggtac
     6781 ccaccagctg cccgaccagg tgatgggccg tccgctgcgg catgccgcgg aggatcagcc
     6841 actccatcaa ggtcgtcgcg tcgaggtgtc cacggtcgag ctgctcggca atcgcctgcc
     6901 gatggagcgc cgtcccagcc accagcggcg cggccaactc caagcataat tcgaccgtat
     6961 cgaaagagtc gaaaatccgc tctttgtctt cctgcaaatc gcggttgtag gccagcggca
     7021 ggcccttcac gagcaccagc agcgactgca aattgccaat cacgcgggcc gtcttgcccc
     7081 gggtcaattc cagcacgtcg ggattcacct tctgcggcat gatcgacgag ccggtgcaga
     7141 attcctgcgg cagcttgagg aagttgaatt ccgcggtgga ccagagaatc cactcctcgg
     7201 cccacgtgct gaggtgctcg gcgatcagcg tcagcacgaa cgccgtttcc aggacgaaat
     7261 cgcggtcgct cgaaatgtcg aggctgttgg ccgcgactgc ctcaaagcca aggcgcctgg
     7321 caacgtcctg gcggtcgatc ggcaggctgc tgccggccag cgccgcggct ccgaggctgg
     7381 agacgttgac ccggcggcgg cagtccatga cgcgcaggcg gtcgcgctgg agcttctcgc
     7441 aataggccag ccaatagtgc ggagcgagca ctggctgggc ccgctggagg tgtgtatagc
     7501 ccggcaggat cacatcgaaa tcgtcatcgc aacggccgat aaaagcacgc tgcaactcca
     7561 aaatcttgga atcgacgcga tctagggcgt cgcgaatcca cattcgcagg tcggttgcca
     7621 cctggtcgtt gcggctgcga cccgtgtgca acttgcggcc gacatccccc aggcgctcga
     7681 tcagcgcctg ttcgatgtgc atgtgaatgt cttccaactc cgtgcggagc ggaaacttgc
     7741 cggcttcgat ttcgccccga atctgctgga gcgtgtcgca aatctggcgg cattcctgct
     7801 catttatcag gccgacggcc gccagcatct gagcgtgagc gatcgagccc gcgatgtcgt
     7861 gggcgaaaag acggtggtcg aagctgacgc tttcggtaaa ccgttcgacg cggcgatccg
     7921 tcgccttgga aaacactccg ctgcgggaag gagtggccaa caatgccccc tggtccgacc
     7981 ggcggctgcc gccaggctgc ccgtcgatgg ttaccggcga gatagaagca accctttcat
     8041 gctaaatggg ttacgtcgtg ctgtcaacga gccgtagatt cgcgcgatga agaatcaggc
     8101 agcgctggag aggcgatttg acctgatatt aggcagctac tgggacaact tgaaatcgac
     8161 gggcgtagaa tgaacatttg gttccgcatt tgctgtaagt aagtcgccag cgcagctact
     8221 gtcattttga ctataaagtt cacaacagcg acgatgatcc agttatgctc ccctggaatg
     8281 gttgtttgcg cataatgcgc atcattgccg ccgcgccgag tttgatcaaa gtacttatgc
     8341 agcaatgggt tacggccatt tttgcagctc caaacttttt cgcggtgatg cgcgcatgaa
     8401 ccggcccccg cgaccggcac aaaccgcgcc gggttcggtt gcgtactggg tgaagttgtc
     8461 gcccccagac agcggttcta caatcaaccg cttggcagct aacgacttag cacgatttcg
     8521 atcgtgagat ccgacatgta ccgatttgct cgctggacta tttggatttc gctgatcctg
     8581 ctctgcctgc ccctggcggc gcaggacgcc cccaccaatc cgctcgacga gccgctcccc
     8641 aagctcaaca tcgccggtca actgggaatc ggcgatgctg gtggagaaaa ggtgacgctc
     8701 actggctcgt tcacgatcga gaagggatcg cggcgcggat ttttgaccat cacggcccag
     8761 atcgagcccg agtggcacat ctattccatc acccagcccg acggcgcaac gcaacggacg
     8821 cgcctcgagc ccgccaagtc agccgactac aagctgctcg ggccgttcca gcccgacatc
     8881 gcgccgaaga tcaagaagta cccgtattgg aaggtccccg tcgaagagca cgaggggcaa
     8941 gtcaagtgga cggctccgat cgagattgcc gcgggagtga agcctgagtc gctgacgatc
     9001 caggtcaagg ccagcggtca ggtctgcaaa gacaactgcc aggacttcgg ttccgacatc
     9061 tcggctgcgc tggccggcta cagcgagtcg tcggccagcg cgggcgaata tcagcctccc
     9121 agcggcgatg cgcagttggc gctcaagggc cacctcgagc cggcggcagc cgcaccgggc
     9181 agcaaggcca ggctggtcat cacggcgatt ccgaacgaag gctggcacgt gtatccctat
     9241 tccccgcagg atcccaatca ggtcggcgtc aataagccaa cgctcattca cctcaatccg
     9301 ctgacgccgg gctggacgcg gtcgcccgtc gtcgaatcaa cgacacccaa agggggacac
     9361 cacgaagagc ctgtgtcttg gacgatcgaa ctgtcgattc cgggcgactc gcccgccggc
     9421 gagagcgtgg tcagcggggc catcgggttg cagacgtgca accccggaag gtgtctgcca
     9481 ccttacgcgg tccggttccg cgccgccctg ccggtgaagg cgaaggccga ggaagggaga
     9541 atcccgctcg agttcattcc cgtcgattcg gccgacaagt cggcgacgat caagggctac
     9601 aacgacgttg cgaaactggc ggccgagcat cccgcgccga ccggcaaagt caatttcgcc
     9661 acactgctgc cgatgatcgg cttcggcctc ttgggcgggc tgattctcaa tctgatgcct
     9721 tgcgtgctgc cggtgatcgg tctcaaagtg ctctcgttca tccagcaggg cgggcaaagc
     9781 cgcggaagta tcttcgcact gaatgtctgg ttcgcgctgg gactgctgtc cgtgtttgta
     9841 gcgctggcga ccctggccgc gttcggctcg ctgataccgc agctcggcca gaatctctcg
     9901 tgggggcagc agttcacttt cacgagcttc aaggtcgcga tggttgtggt ggtgttcgca
     9961 ttcgccctca gcttcctcgg cgtctgggaa gtgccgattc ccggcttcgc ccagtcgcaa
    10021 gcttccggta agttgcagca gcaagaaggt cccgcggggg cattcttcaa gggcatcttc
    10081 acgacactgc tcgccacgcc ctgcagcggg ccgtacctgg gtctggtctt cggttacacg
    10141 ctggcccagc cgccgctggc gacctacctg atcttcacga gcgtcggcct cgggatggct
    10201 gcgccttacc tgctcatcgg ggcgtttccg ggcctggtcc gctggctgcc caagccgggc
    10261 gagtggatgg agaccttcaa gcaggtcatg gggttcgtgc tgctcgggac ggtcgtgtat
    10321 ctgttcgcca cgatcagcgc cgattacttc attccgacgc tggccctggt gatcggcgtc
    10381 tggttcgcgt gctggctcat cggccgcgtg ccgatctacg aagacaccgg caagcagctc
    10441 cgccaatggg cgatcggcat cgccgcggcc gccgtcaccg gttggctggc gttcacttat
    10501 ctggggccgg tcaagcacct ctacgagtgg cagccctaca cgcctgaggc aatcgcaaag
    10561 ctacagaccg aaggcaagac ggtgatggtc gattttacgg ccgattggtg cttaacttgc
    10621 caggcgaact tcaggctggc catcaacacc caccgcgtga aagaggtcgt cgaaaaaaac
    10681 caggtcgtgc cactgctggc cgattggacg gacaagaacg acacgatcaa gcagaagctg
    10741 gccgacttga acagcaattc gattcccgtg ctggccatct atcccgcggg caagccgggc
    10801 gaagtcatta tcctccgcga cgcgctgttg gagcggcagg tgatcagagc gctagaagag
    10861 gccgggcctt cccagccggc ggccgcggct acagcaatgg tggagaatcc gactggcgga
    10921 taatcgcggc tttgaatcag cgcttgcgcg gcttgccggc gacggcggcg cagcgcttgc
    10981 agcgcttgac ctggttgttc agcttcgcgc cgcacttggg gcagcggcgg tttttcagct
    11041 gcatcgtgaa cttggtgcgg gggcgaattc ccatggcgat cgggccggca gtatgcgtgg
    11101 cttcaggtaa gccccgcaat ataggaacag accgccgcgc cggcaatggg cacacgtccg
    11161 aggccgtgct acttgggcaa ctctttggcc gcttccagtg gggccagcgt cgccagcaca
    11221 aacaagcctt gcacaagcaa gagccaggcg acgtcggcaa agctcgcgcc cagattgccc
    11281 ttgaacatcg ccgtcatcgc cagcggcggg accagaccta cgaagaacag agccaggccc
    11341 gcgaccagca gccgcacggt ccactcgcgg cggaggcgca cggccacgag caggctgacc
    11401 gccagcgccg cattggagac gccgatcatc tgcagctgat agaaccactg ccaccgcaag
    11461 acgtcggcca gattccagcc caaggcctgg gccaggcggc ggccagcgcc gagcagcacg
    11521 ccgacgaccg ccgtccagag cagcagaatg aggatcgtga agcggctccg ctcggccacg
    11581 cccggcggcg cgacgagcag ctccagcagg gccgcgccca agccgacgag cacgacttgc
    11641 gtcgccaagc tcgcggccca agcggcggcg gcggtttcgg ccaggtccgt cgactcgagc
    11701 acgccgatca gcagcgccca gagcgccgcc gtcgccagca ccgcgaccag cgtcttggca
    11761 taggagggcc agttcgaacg agcccaccag atcgctccga gcgaacagtg cgccatgacc
    11821 agggccaccg ccgcgagcaa gagcggcggt ccctggggca gcgccggcac gcggccggct
    11881 gcctgggccg cggcgtggac ggctgcggcg agtcccagga tcgccgcatg cagtaccacc
    11941 gccgtgccga tcgccagcac gcgccgctcc agcgggctga tgttcatcgc gcggcctcca
    12001 gctccgcggc caacagcagc agcacctgct cgacaaccca tcgctggcga gtcgcccgcg
    12061 aatcacggcg cccgcactgg agccggcgat gccggacgcg ctgcggcgag cgccaagcga
    12121 cagccgcgaa gaccagcccg tcgagcttcg gcggggcgtt tggccccaag tggcccgtga
    12181 cggacgccgc caggtcggcc tcgggcgtca tgtgcagcac gcgctgggcc atttgctcgg
    12241 caacctcgcg gctcaccggc ccgggtttct cgagcaactc cgccgcgatt cccaggtagg
    12301 cctgtttggt ctggttgcga tagacgacga ctccgccgca atgatagttg gagattccgg
    12361 gaatcttcgt cagcgatccg ctcaccagcc ctcccgtgca gctctcggcg aacgccacct
    12421 tgcgatccag ccgcttcagc aagcgggcaa cagagcggac agcagtcgca atcgacatga
    12481 aatggcttgt ctcttctgaa ccgtgaaccc agaaccctga accctcggta gaatgtgctc
    12541 cgtgaatcct cgccagcaac tcgagccgcc agttgccggc ctcgtcgatc cgcacttgtt
    12601 cggcacgctc tgcgggctgt tctcggcact catttatacc tgcgcgaatt cgttcctgcg
    12661 ggcggtgcac cactgcgatc cggtctgggt ttccacgatc cgcgccgttc cgacggtcgc
    12721 gattatggcc ccggtgctgg ctttgctggc ctcgcgcggt cggcctgtcg taccgcggcc
    12781 gagatatctg ctgctgattg ccgtgggcgg actgtgcggc cagctgggcg gcaacatctg
    12841 ctttcaatcg gccctgcaca tcattggcgt ggccctggcg gtgccgctgt gcatgggcgg
    12901 gatgattgtc tcggcggcga ttctgggccg cgtcttcctg gccgagccgc tgacgccgcg
    12961 ggtgctgttg gcgctggcga tcttgctcgc ggccatcgcc gtgctcgcga tcggcgcggg
    13021 cgaagccggc cgggcgatgc aatcgagcgc ggacagcctg cccgcgcccg cgccgccgtg
    13081 gcggctggcc gccggggtcg ccgccggctg cgggtcgggc gtgttttaca cgctgctcaa
    13141 caccatcctg cggtattgcg tgacgcgcgg agcgccactg cccacatcgc tattcatcgt
    13201 ctccaccgtt ggcgtgatcg gcctgggcag catcgcctgg ctccgtatcg gcggcgcggg
    13261 catgcgggcg acgttgcccg ccgacctggc gatcatgctg gccgcaggct tgtgcaacac
    13321 ggtggcgttc ctggcgctga cgaaatcgct ccagctcaac agtatcgtct acgtcaatgc
    13381 cttgaacgcc acgcaggcca cgctggccgc gatcgcgggc gtcgtcatct ttcgcgagaa
    13441 gctttcgccc tggctggtcg tcggcatcgg cctgacgatc gtcggcctga tggccctcgc
    13501 ccgcgctcac cgcgcgctcc gcacgccggg cgaatccgcg tcatgagccg gcgtcggcct
    13561 gcgcccgctg acgtttactc gcagcgactg gaatcgctgt cgtcgggctc agggatgagc
    13621 atggcgatgg gcaaaacgaa gacgagccag gtcagcacga acgtgaggcc gagcagcgcc
    13681 agggccaacg cggggatgcc cagagccagg aacaacccgt tctttacgtg cggtcccagc
    13741 ccgccggtca ggccgccgat cagcggcggg acgactgcca cgaccagcag aatcgtccgc
    13801 aaggtgaatt gatgccgtga gggagcgccc atccaacgtc cggctgctgg agactcacgc
    13861 attgtatgga aattccgcca attggcagcc cgcggcctgc tatggcatac tggctgtcgc
    13921 gactttgaac tttgaacttt gaacctcgaa ctcctgcaat gcccgccgag actgtaacgc
    13981 ccccctccgt caagccgcgc cgcgctgccg ccacgcaact tccgacgttc gtgttcgaat
    14041 ccagcgacga cctggcccgg cacgtcgcgc agctggtggc cggcgtaatc cgcgagcgga
    14101 acgcccacgg gcaaatggca gtcctcggcc tgcccacggg cagctcgccc atgggcgtct
    14161 atcgcgagct ggcccggatg caccgcgagg agggcctcga cttctcgcag gtcgcggcgt
    14221 tcgtgctgaa cgagttctac ggcgtcgcgc cggaccaatt gcagagccat caccgctgga
    14281 ttcgcgagca ccttctcgat cacatcaaca tcccgcccgc caacgtgcat gttccctgcg
    14341 ggaccacgcc gctggccgag gtcgaaaatc actgccgcga tttcgagtcc cggatcgagc
    14401 acgctggcgg catcgatgtg ctcctcttgg ggatcggccg caacgggcac atcggcagca
    14461 acgagccctt tcccggcgtc ggcggccgca cacggctctc gacgctcgac cccgtaacgc
    14521 ggcgagcgct ggccagcgac tttttcggcg agtggaacgt gcccacgcag gccctcacga
    14581 tgggcctggg cacgatcttc gacgcccgca aggtcctgct gctggcgcag ggcgagcaca
    14641 aggccgagat catccgcgag gtggccgaag gaccagtctc gccgcgcgtg ccggcgtcgt
    14701 acttgcagga gcacgccgac gcggcagtgc tggtcgatag cgcggcgggc agccagctca
    14761 cctcgagcca gacaccctgg gcgatcagca acgtccagtg gaccgacgcg ctcatcaagc
    14821 gagcgatcct ctggctctgc ggccagacgg gcaaagccct cttgaagctg accgatgaag
    14881 atttccgcaa ccacaacctc catcagctgc tgcggcacca tggccccgcg cccaaggtcg
    14941 cgcaccaggt gttccagcag ctcatgggga cgatcgtcta tcacccggcc ggcaaggagc
    15001 cgcagcgggt gatctgcttc agcccccatc cggacgacga tgtgatcagc atgggcggga
    15061 cgctgatccg cctgatcgag gacggtcacg agacgcacat cgcctacatg accagcggaa
    15121 acatcgccgt cttcgaccac gacgccgccc gcgtcgccga cctggtcacc gagtacaacc
    15181 gcctgttcgg catcgaccag gagcggagca agctggtcga agggacagtg ctggaatcgc
    15241 tgcggcgaaa aactcccggc gaacccgata tcgaggccgt gcaaaagatt aaggggctca
    15301 tccgctgggg cgaggcgaag gccggggcgc tcaaagtcgg ctgccgcgag gagcacctgc
    15361 acttcctcga cctgcccttc taccggaccg gcacgatcgc caagaagccg atttgtgacc
    15421 acgacgtgca gatcatccgc gaccttttgg cccgcgtgca gccgcagcag atttacgtcg
    15481 cgggcgacct gtccgatccg cacggcacgc accgggtctg tgcccaggcg attttccggg
    15541 cggttctgga gcacgaggcc cagcacggca gccggccgca agtgctgctc tatcgcgggg
    15601 cctggcagga gtacgagctg ttcgagatcg agatcgccgt gccgctgtcg ccgggcgaca
    15661 tcctgaagaa gcggaaggcg atcttcatgc accagagcca gaaggacgag gccctctttc
    15721 ccggcagcga cccgcgcgag ttctggcagc gggcggagga ccgcaacacc aacaccgccg
    15781 cccagtacaa ccagctcggc ctgcccgagt atttcgcgat ggaggcgttc gtccgctgga
    15841 atggcgtgcc gatttgacgg aggcattcgc cgcaggttcg ttcgagccac cggaggaggc
    15901 gatgacgatc gacgagttca tcgctcgcat cgaagcgacg gcgcggccga cagccgctgc
    15961 ctgatggacc gcccgctgct acattcgtca tgaatggcaa tcagcaccgc agtcctgttg
    16021 ccgggtcttg acggaactgg tgagctgttc cggccgttcg tcgaagctgc tccgcggggg
    16081 atcgaaaccg ccgtcgtgga ctatcccacg agcgaagctt ccattgagat tctggagcgt
    16141 cgagcacgag agaagctggc aactcgctgc atcgtcattg cggaatcatt ctcaggaccg
    16201 attggcgttc ggctggcagc agacgcgcgg gtccaggcac tggtattgtg taactcgttc
    16261 gtcagcagcc ctgcttgctc gctgctgcgc catctgaccc ttgcgcccct gttcgcaatt
    16321 ccgatcccta ggttcatgct ccgcctcctc ctgctgggtc gccaggccag cccaaccctc
    16381 gtcgagaaca cacggcgtgc catccggcgt gttccggcaa cggtgatcgc gcgacgagtt
    16441 cgccaggtcc ttcagacgga cgagcgggaa acagttcgag ctctggaaaa acccgtcctc
    16501 tacttgcgcg gacgcagcga tcacctggtc tcggaacgca gctggaggga cctgcaggca
    16561 gttcgtccgg atgctcaaat tgccagaatc gaaggtccgc atatgctgct tcaggtatcg
    16621 ccgcacgaat gctgggaagc gattgtcggc ttcaccaatg gcatccgcgg tggtctaaca
    16681 ccggcataaa atggcgcggt gacgcagaga ggcgaataac aacgagccgc tctgcctcgg
    16741 acgacgaaat cgggcggccg gctaaagtac ggactggcca accccctcga cttcgacgcg
    16801 gtgctggctg ccctgcgcga atgttacggg aacctggtgc accagtagtg cccgccgcgg
    16861 gagggcgaat ggaaaaaaca ggaagcttct atcagcgagc cggcctggga aaacacacac
    16921 agccggcgag tgcgggcttg ctcgcctggc tgcgccaagt gggggcgagc gaggcactgc
    16981 tcccggcttt cggtgattcg ctccgcgagg atgttgaaga gttcccgctc gtcttcctgg
    17041 ccgagtcaga gctcctgcgg gtcaacgctc ccggcacagg cgggctctgc tgcgggatcg
    17101 cgcagggcct gctggtcgtc ggggcttcgg ccctgagcgg cgacccggtg gtcatcgacc
    17161 tccgcgaccc ggccggtgca ggcggctatc tgaaccacga gacgatgtgg agcgcggcgg
    17221 acctgcgcgg cgaaatctgg cgggcggccc actcctggga gcaactggcc gtgcttatcg
    17281 atcagtgcaa gtttccgagc aactatcacg atgcgaaaca gtccggtccg ccgggcacct
    17341 agccgagtac gaggaaacca cgataaacgt tgaccgatcg agagcttctg atacgatcca
    17401 gtttggttct ttggttcttg gttcttgaga tttgcatgct cacgctccac tccagctcgt
    17461 cgcgcgactg cagcggcctg gcccgccgcg atttcgtgcg cgcaggcgtg ctgggcatcg
    17521 gggcgctggc gctgccgcag cttctggcgg cgaaggcagc cgcgaaggcc gcgggcaagg
    17581 aatacgtgcg ggaccgctcg atcgtgctgc tattcctggg cggcggcgcg agccacatcg
    17641 agaccttcaa cccgaacatc gatgcccctg cgcccttcgc ttcggttacg ggcgaagtca
    17701 aaaccaaggt gcccggcctg acgctcggcg gcacgtttcc gcaattggcc aagcatgctc
    17761 agaagctggc cgtcgtccgc tccttctcgc acgagaacgg caaccacccc aaggcaatca
    17821 ttcacgtcct ctccggcggc accgatccga ccggcgatca gaaggcgggg cagagcatgg
    17881 gctcggtcta tgcccgcctc cgcggtgcga accacgaatc cagcggcctt ccgacctatt
    17941 gcctcctgac ctccgacgag gtcgatggac aataccgcaa cgagaagagc cgcatcgccg
    18001 ccggctcctg gccaggccag ctcggcctgg cgttcgctcc gttcgatccg gcgggcggcg
    18061 ggcagctgct caaaaacctc agcctgggcg tctcggccga gcggctggac gaccgcaaga
    18121 cgctgctggc gaagctcgac accgtcaagc gctcgatcga tgcgtcgggc aacctggaag
    18181 ggatcgaccg cttcgagcgg caggccctgg agatgatcgt tcgcggcgca ggtgaagcgt
    18241 tcgacatccg ccgcgaggac cgccgcacgc tggaccgcta cgacaccacg cagttccgtg
    18301 tcggcaagaa ggaattccag ccctcgg
//
LOCUS       DASRJB010000276         6938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_79852, whole genome shotgun sequence.
ACCESSION   DASRJB010000276 DASRJB010000000
VERSION     DASRJB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79852"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFB80_07455"
     CDS             complement(<1..225)
                     /locus_tag="VFB80_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN33638.1"
                     /translation="MQHKPVNLYDYPRFYDLVFGSDWKAEFEFLRAAFTRFADGRVRR
                     VFEPACGTGRLLYRLGAAGYEVSGLDLNPRA"
     gene            352..792
                     /locus_tag="VFB80_07460"
     CDS             352..792
                     /locus_tag="VFB80_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33639.1"
                     /translation="MNITRLWALCVTILAVAFSSGCNPAAKAIGKWEVETDKFQAQLP
                     PTGENALAAVVAGMSKVLQVRAELEFKADQTWKWELSAIGNTQSMSGTWRYVKTEGDT
                     LVLAAKSSGGEEQEFKLKFVDKDHIEASGLMGNQSFPLKRKKEN"
     gene            832..1278
                     /locus_tag="VFB80_07465"
     CDS             832..1278
                     /locus_tag="VFB80_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33640.1"
                     /translation="MTSCLRFFTCFTLLLVAAGLVGCGGSGLPAPVKRLPGKWHGQMI
                     VFEEEVQGKYTPEQIADFQLWQMDFEFRPDGSMLIAGVTKGQSTSSEGSWEMVKQEGD
                     LLTIKSTEAGGEQKQINCEFDGADTFYMPIKTDVAELGAMRFTRLR"
     gene            complement(1279..2412)
                     /locus_tag="VFB80_07470"
     CDS             complement(1279..2412)
                     /locus_tag="VFB80_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZN33641.1"
                     /translation="MTAEKPTSSNYSPGLEGVIAGETAISTVETGLTYRGYAIEDLAA
                     NSTYEEVAYLILYGELPTAAQAAAFRQRLGAAAAVPQEIIETLRRIPHAAPSMDVLRS
                     AVSLLAHWEKEMGDNSHAANLRKTERLLAQAPVIVAARQRLMTGQEPVAADPSLTLPA
                     NFLWMLRGQKPSPKQVEALDVSLILYAEHEFNASTFSARCVVSTLSDLHSGITGAIGA
                     LKGPLHGGANEAALKVLQEVGSPDRAEAWVRGALARKQKIMGFGHRVYKDGDPRAVYL
                     KRLTAAVAAETGQHDMEKMAEIIESIVWSEKKIPPNVDWPCARLYHYFGLPADLFTPL
                     FVVARIVGWSAHIIEQLDNNRIVRPRSIYKGPSVRKWKPVEQR"
     gene            complement(2421..3287)
                     /gene="prpB"
                     /locus_tag="VFB80_07475"
     CDS             complement(2421..3287)
                     /gene="prpB"
                     /locus_tag="VFB80_07475"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZN33642.1"
                     /translation="MSPGQKLRELVAESAIQVPGAPNALLARLIERLGYEAMYLSGAA
                     FSNGVLGLPDVGLFTLTELALETGYLTRASSLPLIVDADTGFGDVLNVERTVIELRAA
                     GAAAIQLEDQQSPKRCGHLAGKSLIEPAAMCAKLKAAVAAREDDDLVLIARTDARGVT
                     SLDDAIERAKRYIGAGADWIFPEALVSKDEFARFADAIDAPLVANMTEFGKSPLLTLQ
                     ELADLGYSIALYPVTLQRITLKACEAALNLIASEGTQADILDLMQTREELYDLIDYPA
                     FEERDRSYSTDK"
     gene            3353..5005
                     /locus_tag="VFB80_07480"
     CDS             3353..5005
                     /locus_tag="VFB80_07480"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HZN33643.1"
                     /translation="MRRPAASATLFTLDANWRLAAFEVVWIFLIFFLVAGSQPPDVGE
                     SHYLVKAKHYWQPAWCAGDLFCESHDAHGVFYWTLGWVTRIASLTATAWIGRAVTWGL
                     LAWSWRRLSWALVPQPLVSLLSAGLMLLLLRNFHLAGEWIVGGVEAKGFAYVLVFLAL
                     EAIVRGSWRAALLLAGAAGAFHVLVGGWTVVAIGAAWLLAGKERPAAKSLLPAAIGGL
                     VLSLPGLLPALLLNRGTDGAVSVEAARIYVFERLPHHLVYHRFTAAHIWRFAALVAAW
                     LALAIALRGDEKLRRLNLVVAGAALLGLIGVALDQSLALWAKTSAQTAEAYEQAAAPL
                     LRYYWFRLSDSFVPIGAALAIVAGLQQLQAARPAWASWLSAAAILLAGANVADVYYWR
                     HRQPLPGAILQSRPTADAAARWWHSPRAKAPPGEISARAWFRDWQAVCTWIDEHTPKT
                     AKFVTPREQQTFKWYAGRAEVANWKDVPQDARGILEWKAAMQALRTRTDLAQLARDFG
                     AQYVLIDTTRTGRPQGLRRIYPLAREENPTFEVYRLPEPLAP"
     gene            5002..5640
                     /locus_tag="VFB80_07485"
     CDS             5002..5640
                     /locus_tag="VFB80_07485"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZN33644.1"
                     /translation="MKGWEQLEPPAFAAALRGKIARGLPGRSAQRTMAHRLAYGRHHG
                     PVPADARQAAVLLALHQTAQGWSIPAIVRPAEMRAHGGQISLPGGMIESDETPTQSAL
                     REFEEELGASSAALEVIGQLSPVYVFVTGFQVTPLVAVSPTPLEFRPNPHEVARVVEL
                     PLAALLDRANRGQHAIERRGLAFSVPHFAVAGERVWGATSLILAEFVALIAV"
     gene            5713..6723
                     /gene="argC"
                     /locus_tag="VFB80_07490"
     CDS             5713..6723
                     /gene="argC"
                     /locus_tag="VFB80_07490"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZN33645.1"
                     /translation="MIRIGILGATGYTALELIKLLLRHEHAKIEAITSRQEGRPHVSA
                     IHPSLAGRLDMHLEDLGPEQVAERCQCAFLCLPHAASAESAKALLARNVRVIDLSADY
                     RLNDAATYKKWYEHDHPDPERLGKTPYGLPELFAAEIPQARLVANPGCYPTSAILPLA
                     PLLKAGWIEPDDIIVDSKSGVSGAGRTPKLNTLFPECNESISAYSVGKHRHQPEIEQI
                     LGRATGQSVSVIFTPHLTPMDRGILSVIYARPAKQVTEAEVLSLLREFYAGKPFVRIV
                     NHLPATKDTAHTNYVDITARVVKGRVLLISAEDNLVKGASGAAVQNFNLMFGFPETTA
                     LL"
BASE COUNT         1130 a         2250 c         2351 g         1207 t
ORIGIN      
        1 cgcccgcggg ttaagatcga gccccgagac ttcgtaaccc gccgccccca ggcgatacag
       61 cagccggccc gtgccgcagg ctggctcaaa cacgcgccgc acccggccgt cggcaaagcg
      121 ggtaaaggct gcccgcagaa attcgaactc ggccttccag tcggagccga aaaccaggtc
      181 gtaaaatcgc gggtagtcgt agaggttgac aggcttgtgc tgcatagggc cgaatataga
      241 gtggcaggtc cgcgctgagc agggctagcg gcagctcgct agcgggcccg tgcaaatgca
      301 gttgccgcta gcccgtgcct tccgctagat tgccgcgcga gtgagagaac catgaatatc
      361 acgcgtctct gggccctttg cgtgaccatc ttggccgtcg ccttttcctc cggctgcaac
      421 ccagccgcta aggccatcgg caagtgggaa gtcgaaaccg acaagttcca ggcgcagctg
      481 ccgccgacgg gcgagaatgc cctggcggcc gtcgtggccg gaatgtcgaa ggtcctgcaa
      541 gtgcgggccg agttggagtt caaggccgac cagacgtgga agtgggagct gagcgcgatc
      601 ggaaacacgc agtcgatgtc gggcacgtgg cgttacgtca agaccgaagg cgacacgctg
      661 gtgctggcgg ccaagagttc tggcggcgag gagcaggagt tcaagctcaa attcgtggac
      721 aaagatcaca tcgaggccag cggcctgatg ggcaaccaga gctttccgct gaagcgaaag
      781 aaagagaact gagccccgaa aatgcaaccc tgcgatcaag gaggatcgat catgacaagc
      841 tgcttgcgtt tcttcacctg tttcacgctt ctgctcgtgg ccgcgggact ggtcggctgc
      901 ggcggctcgg ggctgcccgc gccggtcaag cgcctgcccg gcaagtggca cggccagatg
      961 atcgtgttcg aggaggaggt gcagggaaaa tacacgccgg agcagatcgc cgacttccag
     1021 ctgtggcaaa tggacttcga attccgcccg gacggctcaa tgctgatcgc cggcgtcacc
     1081 aaaggccaat cgacctccag cgagggcagc tgggagatgg tcaagcagga aggcgacctg
     1141 ctcacgatca aatcgaccga ggccggcggc gagcagaagc agatcaactg cgagttcgac
     1201 ggcgccgaca cgttctacat gccgatcaag accgacgtcg ccgaactggg cgcgatgcgg
     1261 ttcacgcggc tgcggtagct accgctgctc caccggcttc cacttgcgga cgctcggccc
     1321 cttgtagatc gaccgcggcc ggacgatccg gttgttgtcg agctgctcga tgatgtgggc
     1381 cgaccagccg acgatgcgag cgaccacgaa cagcggcgtg aacagatcgg ccggcaagcc
     1441 gaaatagtgg tacagccggg cgcagggcca atcgacgttg ggcgggatct tcttctcgct
     1501 ccagacgatc gactcgatga tctcggccat cttttccatg tcgtgctggc cggtctcggc
     1561 cgcgacggcg gccgtcagcc gcttgagata gacggcccgc ggatcgccat ccttatagac
     1621 gcggtggccg aagcccatga tcttctgctt gcgggccagc gcgccgcgga cccaggcctc
     1681 ggcccggtcg ggggagccga cttcctgcag caccttcagg gctgcttcgt tcgcgccgcc
     1741 gtgcagcgga cccttcaggg cgccgatcgc cccggtgatt cccgaatgca ggtccgacag
     1801 cgtcgaaacc acacagcggg ccgagaacgt gctggcgttg aactcgtgct cggcatacag
     1861 gatcagcgag acgtccaggg cctcgacctg cttggggctg ggcttttgcc cgcggagcat
     1921 ccacaggaaa ttggcgggta gagtgagcga cggatcggcc gcgacgggct cttgccccgt
     1981 catcagccgc tggcgggcgg cgacgatgac cggcgcctgg gccagcagcc gctcggtttt
     2041 gcgaaggttg gcggcgtgtg aattgtcgcc catttccttt tcccaatggg ccagcaggct
     2101 gacggccgag cggagcacgt ccatcgacgg cgcggcgtgc ggaattcgcc gcagcgtctc
     2161 gatgatctcc tggggcaccg cggccgccgc gccgagccgc tggcggaagg ccgcggcctg
     2221 tgctgcggtc ggcagttcgc cgtagaggat caggtaagcg acttcctcgt acgtcgagtt
     2281 ggcggcgaga tcctcgatcg catagccacg gtaggtcaat cccgtctcga cggtgctgat
     2341 ggccgtttcg ccggcgatga cgccttccag gccgggagag taattggatg aggtcggttt
     2401 ttcggcagtc atggtgctgg tcacttgtcg gtcgaatagg aacgatcgcg ctcctcgaac
     2461 gccgggtagt cgatcaggtc gtacagttcc tcgcgggttt gcatcaggtc gaggatgtcg
     2521 gcctgcgtgc cttcggaagc aatgaggttc agggccgcct cgcaggcctt gagcgtgatt
     2581 cgctggagcg tcacaggata cagcgcgatc gagtagccca ggtcggccag ctcctggagc
     2641 gtgagcagcg ggctcttgcc gaactcggtc atgttagcaa cgagcggtgc gtcgatcgcg
     2701 tcggcgaagc gggcgaactc gtctttgctc acgagcgctt cgggaaaaat ccagtcggcg
     2761 cccgcgccaa tgtagcgctt ggcccgctca atggcgtcgt ccaagctcgt cacgccgcgg
     2821 gcgtcggtcc gggcgatgag caccaggtcg tcgtcctccc gcgcggcgac cgccgccttc
     2881 agcttcgcgc acatggccgc cggctcgatc agcgacttgc cggccaggtg gccgcaccgc
     2941 ttgggcgatt gctggtcttc gagctggatc gccgccgccc cggccgcccg cagctcgatc
     3001 accgtccgtt ccacattgag cacgtcgccg aatcctgtgt cggcatcgac gatcagcggc
     3061 agactgctgg cccgggtcaa atagccggtc tccagagcga gttccgtgag cgtgaacagg
     3121 ccgacgtcgg gcaggcctag cactccgttc gaaaacgccg cccccgacag gtacatcgcc
     3181 tcatagccca agcgctcgat taggcgcgct aatagcgcat tgggcgcgcc gggcacctgg
     3241 atcgcgctct cggcgacgag ctcgcggagc ttctggccgg gggacatctt gccgatgata
     3301 ggccgccccg cactgtctgg ccagaatggg cagccaagag tagtcttgat ggatgcgacg
     3361 gcccgcggcg agtgccactc tctttactct cgacgccaac tggcgtctgg cggcgttcga
     3421 ggtcgtctgg atcttcctga ttttctttct cgtggccggc tcgcagccgc cggatgtcgg
     3481 cgagtcgcac tatctggtca aggcgaagca ctattggcag ccggcgtggt gtgcgggcga
     3541 cctgttctgc gagtcgcacg acgcccacgg cgtgttttac tggactcttg gctgggtgac
     3601 gcggattgcc tcgcttacgg ctaccgcctg gatcggccgc gcggtcacct gggggctgct
     3661 cgcctggtcg tggcggcggt tgagctgggc gctcgtgccg cagccgctcg tctcgctgct
     3721 ctccgcgggg ctgatgctgc tgctgttgcg aaactttcac ctggcgggcg aatggatcgt
     3781 gggcggcgtg gaggccaagg gttttgccta cgtactggtg ttcctggcgc tggaagcgat
     3841 cgtccgcggc agctggcgag cggcgctgct cctggcgggt gcggcggggg cttttcatgt
     3901 gctggtcggc ggctggacgg tcgtggcaat cggcgcggcg tggctgctgg ccggaaaaga
     3961 gcggccggcg gccaaatcgc tgctgcccgc ggcgatcggc gggctggtgc tgtcgctgcc
     4021 ggggctgctt ccagcgctct tgctcaatcg cggcaccgac ggggccgtct cggtcgaggc
     4081 agcccggatt tacgtcttcg agcggctgcc gcaccacctg gtctatcacc ggttcacggc
     4141 ggcccacatc tggcggttcg cggcgctggt ggccgcctgg ctggcgctgg cgatcgccct
     4201 gcgcggcgac gagaagctgc ggcgcctaaa cctggtcgtg gcgggcgcgg cgctcttggg
     4261 cctcatcggc gtggcgctcg accagtcgct ggcgctatgg gccaagactt cggcgcaaac
     4321 cgcggaagcc tacgaacagg cggccgctcc gctcttacgc tattactggt tccggctgag
     4381 cgattcgttc gtgccgatcg gcgcggcgct ggcgatcgtc gccggcctcc agcagctgca
     4441 ggcagcgcgg ccggcctggg cgagctggct ctccgctgcg gccatcctgc tggccggcgc
     4501 gaacgtggcc gatgtctatt actggcgtca ccggcagccg ctgcctgggg cgatcttgca
     4561 gtcgcgcccc acggccgacg cggcggcgcg gtggtggcat tcgccgcggg cgaaggcccc
     4621 gcctggagag atctcggccc gcgcctggtt ccgcgattgg caggccgttt gcacctggat
     4681 cgacgagcac acgccgaaga ccgcgaagtt tgtcacgccc cgcgagcaac agaccttcaa
     4741 gtggtatgcc gggcgggcgg aggtcgccaa ttggaaggac gtgccgcagg acgcccgcgg
     4801 catcctcgag tggaaggccg cgatgcaggc cctgcggacc cgcaccgatc tggcgcagct
     4861 ggcccgcgac ttcggcgcgc agtatgtgct gatcgacacc acgcggaccg gccgcccgca
     4921 gggattgcgg cggatctatc cgctcgctcg cgaggagaat ccgacgtttg aggtctatcg
     4981 cctgccggag ccactcgcac cgtgaagggc tgggagcaac tcgagccgcc ggctttcgcc
     5041 gcggcgctgc gagggaaaat cgcccgcggc ctgcctggcc gctcggcgca gcggacgatg
     5101 gcgcatcggc tggcgtacgg ccgccatcac gggccggtgc cggccgacgc ccggcaggcg
     5161 gcggtcttgc tggcgctcca tcagaccgcg cagggctggt cgatccccgc catcgtgcgg
     5221 ccggcggaga tgcgggccca cggcggccag atcagcctgc ccggcgggat gatcgagtcg
     5281 gacgaaacgc cgacgcagtc ggccctccgc gagttcgagg aagaactggg cgcatcctcg
     5341 gctgccctgg aagtgattgg ccagctatcg cccgtgtacg tcttcgtcac cggctttcag
     5401 gtcacgccgc tggtcgcggt cagcccgacg ccgctggaat ttcggcccaa tccccacgaa
     5461 gtcgcccggg tcgtcgagct gccgctggcg gcgctgctgg acagagccaa ccgaggccag
     5521 catgcgatcg agcgccgcgg gctggcgttt agcgtcccgc acttcgccgt cgcaggcgag
     5581 cgggtgtggg gcgcgaccag cctgatcctc gcggagttcg tggccctgat tgcggtttga
     5641 tatatttgac gaacatttcc agcgattcgg tgtcttgtcg gtcgctccgc tattcgcagg
     5701 cgaaaacgaa ccatgatccg catcggcatt ctcggcgcta ccggctacac ggcgctggag
     5761 cttattaaac tcctgctccg acacgagcac gcgaagatcg aggccatcac cagccggcag
     5821 gaaggccgcc cgcacgttag cgcgattcat ccgtcgctag cgggccggct ggacatgcac
     5881 ctggaagacc tcggccccga gcaagtcgcc gagcggtgcc agtgtgcgtt tttgtgcctg
     5941 ccgcatgccg cgagtgccga atcggccaag gccctgctgg cccgcaacgt gcgggtcatc
     6001 gatctttcgg ccgattaccg cctgaacgac gccgcgacct acaagaaatg gtacgagcac
     6061 gaccatcccg accccgagcg gctgggcaaa actccgtatg ggttgcccga gctgttcgcc
     6121 gccgaaattc cgcaggccag gctcgtcgcc aaccccggct gctatcccac ctcggccatc
     6181 ctgccgctgg cgccgctgct caaggccggc tggatcgagc cggacgacat catcgtcgat
     6241 agcaagagcg gcgtttccgg agccggccgg acgccgaagc tgaacacgct ctttcccgaa
     6301 tgcaacgaaa gcatttcggc ctacagcgtc ggcaagcacc gccatcagcc ggagatcgag
     6361 cagattctcg gccgggccac cggccagtcg gtcagcgtca ttttcacacc gcacctgacg
     6421 ccgatggacc gcggcatcct gagcgtgatc tatgcccggc cggcgaagca ggtcacggag
     6481 gccgaagtgc tgagcctctt gcgcgagttc tacgccggca agccgttcgt ccgaatcgtc
     6541 aaccatctgc cggcgacgaa agacacggcc cacacgaact acgtcgacat tacggcccgc
     6601 gtcgtgaagg gccgcgtgct gctcatttcc gccgaggaca acctcgtcaa aggcgccagc
     6661 ggcgcggcgg tgcagaattt caatctgatg ttcgggttcc ccgagacgac cgcgctcctg
     6721 taggcggcac aatggcgctg cattccgacc aagctcgggc ggtcttgaaa aagttgggcg
     6781 ccaagaatcg ctcccgccgg cctttcggtc gtgtggacca tcgcctgcct ggcggtgctc
     6841 ggcctcgtcg tcgcctggtg cctgccggtc gagccgctgg cccatacgaa gctcaagcat
     6901 ttgcaggttc atcagatcgt cgccgccggc ttgttcgg
//
LOCUS       DASRJB010000277         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3462816, whole genome shotgun sequence.
ACCESSION   DASRJB010000277 DASRJB010000000
VERSION     DASRJB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462816"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3431
                     /locus_tag="VFB80_07495"
     CDS             <1..3431
                     /locus_tag="VFB80_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33646.1"
                     /translation="GEILELKNTVNTMVDQLRSFASEVTRVAREVGTEGKLGGQAQVE
                     GISGTWRDLTESVNNMAANLTAQVRGIAKVVTAVANGDLKRKLTVEAKGEIAELAETI
                     NEMTDTLATFADQVTSVAREVGVEGQLGGQAKVPGAAGTWKDLTENVNQLAANLTTQV
                     RAIAEVATAVTKGDLTRSITVVAQGEVAALKDTINEMIRNLRDTTQKNTEQDWLKTNL
                     AKFSRMLQGQRDLTNVGRMVLSELCPVVAARQAEFYVFDPTADAPQLKLLASYASEGK
                     HQHGRRINMGQGLIGQCALEKQKIELNNTPDGYVRISSGLGDAPPRSILVLPLIFEGE
                     VRGVLELASFQGFNPSHQALLDQLTESIGIVLNTIEANMRTEDLLKQSQSLAQQLQTR
                     QEQLQNTNEELQEKAHLLAKQNEEVERKNAEVEQARQELEEKARQLALTSKYKSEFLA
                     NMSHELRTPLNSLLILSDQLSKNTDGNLTPKQTEFSKTIHSSGNDLLNLINDILDLSK
                     IESGTVQVDLSELRLGDLRSFVERTFRHVAEAKNVEFAVEFDRKLPETMLTDAKRLQQ
                     IIKNLLSNAFKFTHHGRVSLLVHAADAGWSDSNDDLNRADGVLAFDVRDTGIGIPPDK
                     QQIIFEAFQQADGSTSRKYGGTGLGLAISRELSRLLGGEIRLVSRPGSGSTFTLYVPR
                     NYTPPRTPRKPLAATTPVALAPAAEKPRKRTSKRVAGGKSNGHSRLPAPPEPPQEVSL
                     INEVDDDRDQIRAGDRVLLIVENDMAFARLLLEVAREHGMKGLVTSLGAAALVLARDY
                     KPHAVTLDIFLPDIEGWRVLERLKSDLAARHTPVCVISTEEARARSLASGAVAFVAKP
                     IQSRDVLDGVFDYLGDFISRPKRHVVVVEPDEARRKEICELVGAEGVEISTPDGGRAA
                     ASLLGQKKADCVVVHPGETALAEELSAAQPQAGRGLSQLPVLVYGTETAEAGVWNRLA
                     DRCTLRRVDSRERLLDLTMLFTHRTVGELPADKQQMLVDLHDSDKILASKKVLIVDDD
                     MRNIFALATVLEEHAMETISADNGRDAIRLLQTEPDIDVVLMDIMMPELDGMETIREI
                     RRDPKLRNLPIVAVTAKAMKGDREKCIEAGAWDYLSKPVDTEQLLAVLRAWLHA"
     gene            3486..>4507
                     /locus_tag="VFB80_07500"
     CDS             3486..>4507
                     /locus_tag="VFB80_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33647.1"
                     /translation="MSDDRKAKILVVDDRAENLLVYQTILEELGQDLLTARSGEEALK
                     LVLAHEFAVVLLDVNMPGVDGFETAALIRKRKRSAHTPIIFITAFTDELRVAEGYSHG
                     AVDFIRTPIVPGILRAKVKVFVDLFRMNEEVKRHAEERIAHAQERSKREAVEESNRRL
                     AFLARAGAVITKSLDRGVTIESILRLLVPEYCDQAVLAEPDSAGRWQGTWARPGAPSV
                     PLARSAALAELPKEWRESIHRVMTGGQADHWPPAIAECAPPPSGLLALPLHDRDRIFA
                     VLLMVNETATRRYQQSDVAMFESIAWRSAIALVNSQLYHEIEHADQQKNRFLSMLAHE
                     LRNPLAP"
BASE COUNT          873 a         1494 c         1483 g          657 t
ORIGIN      
        1 agggcgaaat cctggagctg aagaacaccg tcaatacgat ggtcgatcag ctccgctcgt
       61 tcgcctccga agtgactcgt gtggcccgcg aggtgggcac cgaaggcaag ctgggcggcc
      121 aggcccaggt cgaaggcatc agcggcacgt ggcgcgacct gaccgaaagc gtcaacaaca
      181 tggccgccaa cctgaccgct caggtccgcg gcatcgcgaa agtcgtgacc gcggtggcca
      241 acggcgatct gaagcgcaag ctcacggtgg aagccaaggg cgaaatcgcc gagcttgccg
      301 aaacgatcaa cgaaatgacc gacacgctgg cgacgttcgc cgaccaggtc acgagcgtgg
      361 cccgcgaagt cggcgtcgaa gggcagctcg gcggccaggc caaggtgccc ggggccgccg
      421 gcacctggaa ggacctcacc gaaaacgtca accagctcgc cgccaacctc accacgcagg
      481 tgcgggccat cgccgaagtc gccaccgccg tgaccaaggg cgacctcacc cggtcgatca
      541 cggtcgtcgc ccagggcgaa gtcgccgccc tgaaagacac gatcaacgag atgatccgca
      601 acctccgcga caccacgcag aaaaacaccg agcaagactg gctgaaaacg aacctcgcca
      661 agttcagccg catgctccag gggcagcgcg acttgacaaa cgtcggccgc atggtgctct
      721 cggagctgtg ccccgtggtt gcggctcgcc aggcggagtt ttacgtgttc gatcccacgg
      781 ccgacgctcc ccagctgaaa ttgctggcca gctacgcctc cgaaggcaag catcaacacg
      841 gccggcggat caacatgggc cagggcctga tcggccagtg cgccctggag aagcagaaga
      901 tcgagctgaa caacacgccc gacggctacg tccgaatcag ctcggggctg ggtgacgcgc
      961 cgccgcggag catcctcgtg ctgccgctga tcttcgaagg cgaagtccgc ggcgtgctgg
     1021 agctggcttc gttccagggc ttcaatcctt cgcaccaggc gctgctcgac cagctcacgg
     1081 agtcgatcgg catcgtgctc aacacgatcg aagccaacat gcggaccgag gacctgctca
     1141 agcagtcgca gtcgctggcc cagcagctgc aaactcgcca ggaacagctg cagaacacca
     1201 acgaagagct gcaggaaaag gcccacctgc tcgccaaaca gaacgaagaa gtcgaacgca
     1261 aaaacgccga agtcgaacag gcccgccagg agctggaaga aaaggcccgc cagctcgccc
     1321 tcacgtcgaa gtacaagtcg gagtttctgg cgaacatgtc gcacgagctg cggacgccgc
     1381 tcaatagttt gctgattctc tccgatcagc tctccaagaa caccgacggc aacctgacgc
     1441 ccaagcagac cgagttttcc aagaccatcc actcgtcggg gaacgacctg ctgaacctga
     1501 tcaacgacat cctcgacctg tcgaagatcg agtccggcac ggtgcaggtc gatctgagcg
     1561 agctgcggct gggggacctg cggagctttg tcgagcgaac cttccgccac gtcgccgagg
     1621 cgaagaacgt cgagttcgcc gtcgagttcg accgcaagct gcccgagacg atgctcaccg
     1681 acgccaagcg gctgcagcag atcatcaaga atctgctctc caacgcgttc aagttcacgc
     1741 accacggccg cgtcagcctg ctggtgcacg ctgcggacgc cggctggagc gacagcaacg
     1801 acgacctcaa tcgggccgac ggcgtgctgg cgttcgatgt tcgcgacacc ggcatcggca
     1861 tcccgcccga caagcagcag atcatcttcg aggccttcca gcaggccgat ggctccacca
     1921 gccgcaaata tggcggcacc ggcctgggcc tggcgatcag ccgcgaattg tcgcggctct
     1981 tggggggcga gattcgcctc gtgtcgcggc cgggcagcgg gagcacattc acgctctacg
     2041 ttccgcggaa ttacactccg ccccgcactc cccgcaagcc gctggcggcg acgacgcctg
     2101 tcgccctggc gccggcagcc gaaaagccgc ggaagcgcac cagcaaacga gtcgctggcg
     2161 gcaaatcgaa cggccactcg cgcttgccgg ccccgcccga gccgccgcag gaagtcagcc
     2221 tgatcaacga ggtggacgac gatcgcgacc agatccgggc cggcgaccga gtgctattga
     2281 tcgtcgagaa cgacatggcg tttgcccggc tgctgctgga ggtggcccgc gagcatggca
     2341 tgaaggggct ggtgacgtcg ctcggcgcgg cggccctcgt cctggcccgc gactacaagc
     2401 cgcacgccgt gacgcttgac atcttcctac ccgacatcga gggctggcgg gtgctggagc
     2461 ggctgaagag cgacctggca gcgcggcata cgccggtgtg cgtcatctcg accgaggagg
     2521 cgcgggcccg gtcgctggct tcgggggcgg tggcgttcgt ggccaagccg atccagagcc
     2581 gcgacgtctt ggacggcgtg ttcgattatc tgggggactt catttcgcgg cccaagcggc
     2641 acgtggtggt ggtcgagccc gacgaagcgc ggcggaagga gatttgcgag ctggtcggcg
     2701 ccgagggggt tgaaatctcg acccccgacg ggggccgcgc cgccgcgtcg ctcctcggtc
     2761 agaaaaaagc cgactgcgtg gtcgtgcacc cgggcgagac cgcgctggcc gaagagctct
     2821 cggccgcgca gccgcaggcg ggccgcggcc tctcgcagtt gccggtgctg gtgtacggca
     2881 cggagaccgc cgaagccggg gtctggaacc ggctggccga ccgctgtacc ctgcggcgag
     2941 ttgactcgcg cgaacgactg ctcgacctga cgatgctgtt tacgcaccgc acggtcggcg
     3001 agctgccggc cgacaagcag cagatgctgg ttgacctgca cgactcggac aagatcctcg
     3061 ccagcaagaa ggtgctgatc gttgacgacg acatgcggaa catcttcgcc ctggcgacgg
     3121 tgctggagga gcacgcaatg gagacgatct cggccgacaa cggtcgcgac gccattcgcc
     3181 tgctccagac cgagcccgac atcgacgtcg tgctgatgga catcatgatg ccggagctgg
     3241 acggcatgga gacgatccgc gagattcgcc gcgatccgaa gctgcggaac ctgccgatcg
     3301 tggccgtcac ggcgaaggcg atgaagggcg accgggaaaa atgcatcgag gccggggcct
     3361 gggactacct cagcaagccg gtcgatacgg agcagctgct cgcggtattg cgggcctggc
     3421 tccatgcgta accgatcacc ctcaccccag ccctcgtccc cagcgagagg cagcggagca
     3481 gcggcatgag cgacgaccgg aaggccaaaa tcctcgtcgt cgatgaccgg gccgaaaacc
     3541 tgctcgtcta tcagacgatc ctggaggagc tgggacagga cctgctgacg gcgcggtccg
     3601 gcgaggaggc gctcaagctg gtgctggccc atgagttcgc ggtcgtgctg ctcgacgtta
     3661 acatgccggg cgtcgatggc ttcgagaccg cggccctgat ccgcaagcga aagcgctcgg
     3721 cccacacgcc gatcatcttc attacggcat tcaccgacga gctgcgggtc gccgaaggct
     3781 actcccacgg cgcggtcgat ttcatccgca cgccgatcgt cccgggaatc ctgcgggcca
     3841 aggtcaaggt gttcgtggac ctcttccgga tgaacgagga ggtcaagcgg cacgccgaag
     3901 agcggatcgc tcatgcccag gagcgatcga agcgcgaggc ggtcgaggaa tccaaccgcc
     3961 ggctggcctt tctggcccgg gccggcgcgg tgatcaccaa gtcgctcgac cgcggcgtta
     4021 cgatcgagag cattctgcgc ctgctcgtcc cggagtattg cgaccaggcg gtgctggccg
     4081 agccggattc cgcgggtcgc tggcagggga catgggccag gcccggcgcg cccagcgtgc
     4141 cgctggcccg gagcgccgcc ctggccgagt tgcccaagga gtggcgcgaa tcgatccacc
     4201 gggtgatgac cggcggccag gccgaccatt ggccgccggc cattgccgag tgcgctccgc
     4261 cgccgtcggg cttgctggcg ttgccgctgc acgaccgcga ccggatcttc gccgtgctgc
     4321 tgatggtgaa cgagaccgcg acccgccggt atcagcagtc cgacgtggcg atgttcgaat
     4381 cgatcgcctg gcgctcggcg atcgccctgg tcaattcgca gctctaccac gagatcgagc
     4441 acgccgacca gcagaagaac cgcttcctgt cgatgctggc ccacgagctg cgcaatccgc
     4501 tggcgcc
//
LOCUS       DASRJB010000278         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2902038, whole genome shotgun sequence.
ACCESSION   DASRJB010000278 DASRJB010000000
VERSION     DASRJB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902038"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFB80_07505"
     CDS             complement(<1..353)
                     /locus_tag="VFB80_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33648.1"
                     /translation="MKLARTAFAAAIAWILCAAGAWAQVAEPSATRPASFDYFYYNAA
                     AQTAAAQPSPSDQPSPSDQVVPGVKVPVQAPIQAPGCDTCCDSCCGCGWLSNWPCGCP
                     LECLGEACKLWEPCC"
     gene            complement(672..2426)
                     /locus_tag="VFB80_07510"
     CDS             complement(672..2426)
                     /locus_tag="VFB80_07510"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN33649.1"
                     /translation="MRSLALLAATLLSFDSLAAAPRPNIVVIMSDDMGYSDIGCYGGE
                     ISTPTLDKLAAGGLRFTQFYNTARCCPTRAALLTGLYPHQAGIGHMMEDRGLDAYRGD
                     LNRRCATIAEVLRPAGYGTYAVGKWHVTKAARPEGPKDNWPLQRGFDRFYGTITGAGN
                     FYDPGTLTRDNTMISPQIDKEYQPKQYYYTDAISDNAARYIADHHRQSAEKPLFLYVA
                     YTAAHWPMHALEEDIAKYKGTYDAGYEPIRRARFERTKKLGLISSDAQLSATAGNWNS
                     VENKAWEARCMEVYAAMIDRMDHGIGRIVAELEKTGRLNNTLICFLQDNGGCAETVGR
                     QPAMGVPTLRPSQPPLPPQGADFIDTAVRPTRTRDGYQVRMGPGAMPGPGDTFIAYGQ
                     GWANVSNTPLREYKHWVHEGGISTPLVVHWPAGIERRGQLEPQPGHLIDLMATCVAVS
                     GAEYPPAGTTNVQPLEGRSLVPAFAGQPIQREAIYWEHEGNRAVRAGNWKLVAKGPAG
                     KWELYDIDRDRAEQHDLADQQPDRVKQMTAQWEAWAKRAGAIPWIWQPQYGEPPTNGI
                     EPKKTKAAKKGAKGKANP"
     gene            2585..>3065
                     /locus_tag="VFB80_07515"
     CDS             2585..>3065
                     /locus_tag="VFB80_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33650.1"
                     /translation="MQVRCPHCHEMETIGAEEPADITCHSCGSAFNLAPQTLSFWRSK
                     RTIAHFQLQDELGSGGFGTVWKAYDTKLDRLVAIKVPRFDRLDSEPAELILREARAAA
                     QLKHPGIVAVHEVGRDDGMLYIVSDFIAGVTLADRLTAGPLGPREAARIAAEVARAVH
                     "
BASE COUNT          464 a          989 c         1057 g          555 t
ORIGIN      
        1 tcgcagcagg gctcccaaag cttgcaggcc tcgccgaggc attccagcgg gcagccgcag
       61 ggccagttgc tcagccagcc gcaaccgcag caggaatcgc aacaggtgtc gcagcccggg
      121 gcttgaatgg gggcttggac cggcactttc acgccgggca cgacctgatc gctgggcgaa
      181 ggctggtcgc tgggggaggg ctgggcggcg gcggtttggg ccgcggcgtt gtagtagaag
      241 tagtcgaacg aagcgggacg ggtcgcggac ggctctgcga cctgggccca tgcgcccgcg
      301 gcgcagagga tccacgcgat cgcggccgcg aaggcggtac gagcgagctt catggtgata
      361 actcccaatc catgggaaac agggccaggg gcgcgccggc ggtcgccggg cggtgaggca
      421 ggtagaccga atccgtcgtc cgtcctgagc ggggagagcg ccgccacgac agatcggcta
      481 cgttcctatc gggtgctcct tcgcagcgag tgcaccgaac cgcccggata tgccgcgcga
      541 atcgccgcgg tcgttctgca ggtgacgcct gcggaaacct tggcgcgggc gggatattcc
      601 cggcagggag gaccttgccg tgggcacaga cttggaggcg tcaattcttg ctagcgtcag
      661 aggcccggct gctacggatt tgccttgccc ttggcgccct tcttcgctgc cttcgtcttc
      721 tttggttcga ttccattcgt cggcggctcg ccatattgcg gctgccagat ccagggaatc
      781 gcgccggcac gtttggccca cgcttcccac tgcgcggtca tttgtttgac gcggtcgggc
      841 tgctggtcgg ccaggtcgtg ctgctcggcg cggtcgcggt cgatgtcata cagttcccat
      901 ttgcccgcgg gacctttggc gacgagcttc cagttgcccg cgcggactgc ccggttgccc
      961 tcgtgctccc aatagatcgc ttcgcgctgg atcggctgcc cggcgaacgc gggaacgagg
     1021 ctgcgaccct ccagcggctg aacattggtc gtccccgcgg gcggatactc cgcgccggat
     1081 accgcgacgc aggtcgccat caggtcgatc aggtgtccgg gctgcggttc gagctgtccg
     1141 cggcgctcga tcccggcggg ccagtgcacc acgagcggcg tggagatgcc cccctcgtga
     1201 acccagtgct tgtattcgcg aaggggcgtg ttgctgacgt tggcccagcc ctggccgtag
     1261 gcgatgaacg tgtcgcccgg ccccggcatc gcgcccggac ccatgcggac ctgatagccg
     1321 tcgcgggtgc gcgtcggtcg gacggcggtg tcgatgaaat ccgcgccttg cggcggcagc
     1381 ggcggctggc tgggccgcag ggtcggtacg cccatcgctg gctgccggcc gacggtctcc
     1441 gcgcagccgc cgttgtcctg caggaagcag atcagcgtgt tgttcaggcg gccggtcttc
     1501 tccagttcgg caacaatccg gccaatgccg tggtccatgc ggtcgatcat cgcggcatag
     1561 acctccatgc agcgggcctc ccaggccttg ttctcgacgg agttccaatt gccggcggtc
     1621 gctgaaagct gcgcatccga cgaaatcagc cccaacttct tggttcgctc gaatcgcgcc
     1681 cggcggatcg gctcgtagcc ggcgtcgtac gtgcccttgt atttggcgat gtcctcttcc
     1741 aaggcgtgca tcggccagtg cgcggccgtg taggccacat acaggaacag cggcttctcg
     1801 gccgactgcc ggtgatggtc ggcgatgtac cgcgcggcgt tgtcgctgat ggcgtcggtg
     1861 tagtaatact gcttcggttg atactccttg tcgatctgcg gcgagatcat cgtattgtcg
     1921 cgggtcagcg tgccggggtc gtagaaattg cccgcgccgg tgatcgtgcc atagaaccgg
     1981 tcgaacccgc gctggagggg ccaattgtct ttcgggccct cgggcctggc cgccttggtg
     2041 acgtgccact tgcccaccgc gtacgtgccg tacccagccg gccgcaatac ttcagcaatc
     2101 gtcgcgcagc ggcggttcag gtcgcctcga taagcgtcca gaccgcggtc ctccatcatg
     2161 tggccgatgc ccgcctggtg cggatagagg ccggtgagca gcgccgcccg cgtcggacag
     2221 cagcgggcgg tgttatagaa ctgcgtgaat cgcagcccgc ccgcggcgag cttgtccagt
     2281 gtgggcgtgc tgatctctcc gccgtagcat ccgatatcgc tatagcccat gtcgtcggac
     2341 atgatcacga cgatgttcgg ccgcggagca gcggcgagcg aatcgaacga aaggagagtt
     2401 gccgccagga gtgccagaga gcgcatggtt ggtcgccgct atcggaggga ggatcgcagt
     2461 ttcctcttct aaccctcgct gccgccggct gcaatccgcc gggcgtttcg atcgcggcag
     2521 cattgcccgg caagccgctg gcggacgcta caatcgagcg gttcaagcgt ccacgagcgc
     2581 cgccatgcaa gtccgctgcc cgcattgcca cgagatggaa acgatcggcg cggaggagcc
     2641 ggccgacatc acgtgccatt cctgcggcag cgcgttcaac ctggcgcccc agacgctgtc
     2701 gttctggcgc agcaagcgga caatcgccca ctttcaattg caggatgaac tggggtcggg
     2761 cggcttcggc accgtctgga aggcctacga cacgaaactc gatcgcctgg tggccatcaa
     2821 ggtgccgcgc ttcgaccgtc tcgattccga accagcggag ttgatcctgc gcgaggcgcg
     2881 ggccgcggcg cagctcaagc atccgggcat cgtcgcggtc cacgaagtcg gccgcgacga
     2941 cggcatgctg tacattgtca gcgatttcat cgcgggcgtg acgctggccg atcggctgac
     3001 ggccggtccg ctcggcccgc gagaggcggc gcggatcgcg gccgaggtcg cccgggccgt
     3061 gcatc
//
LOCUS       DASRJB010000279         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2902245, whole genome shotgun sequence.
ACCESSION   DASRJB010000279 DASRJB010000000
VERSION     DASRJB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902245"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFB80_07520"
     CDS             <1..1323
                     /locus_tag="VFB80_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33651.1"
                     /translation="WQAVLKAWVKRGIERGVDGYIANYFYKHDCHCEHCQREFRAYMR
                     GRHSAEELKAAYSISDLATLKFEEMAYWHDPAQTTPLKLEMLRWAQISNKQVFDKVFV
                     DYGRSLKKDLIVAQWNHLGDFGQISGDERCLLPADLWGKGEDYLWYSTGGAAAATDIA
                     AGNLGEGTLQARYIRGMFDDKPFTLGKYESTRTRVTIAELAANGGAPMGFYTRFTDPE
                     ARKEIVRYYQFLKRYDEIYRANREHAEFTLHFPRKEMAREAVAAIERVEGFKWLAKAL
                     LDEHVLFAVLSDENAGRWNSPPLRLDRPPDDPARTREGIHTAVQGAIFSRFDAPSTVR
                     ISASRPAQGNELDIHFVNYNRTEPPKGRDGKPSPGGGIKDEQPIEAPPIACDVVVPAG
                     FTAASIEAITPEQPEPVAIQFTAEGDRIRFSLPKFLVYSVARIQLKAK"
     gene            1351..>2235
                     /locus_tag="VFB80_07525"
     CDS             1351..>2235
                     /locus_tag="VFB80_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33652.1"
                     /translation="MFRARGTWLAGFLWIALGPALVTAQESLWIEAEHLDGVRGYCWP
                     MGKPEMKKTDGHWGLSGPGWAAEWNQGGESGFLSIATAADDDQAVATKTIEVPAAGSY
                     QVWVRYGDWRETPERFAVQIEQPGRPVWSGSFGERAVIEEDNEMKLYWGWAFAWDSRP
                     ADLAKGPATLKLVSTTKQQQPRQIDCIVLTTDSAYQPRIKERPKNHAWELLDSYRGRV
                     PPLEPLARRVPDWKQPATYESPAAWKLHTFRDQEFLYLWNVSHTNAAETWLSDKPERI
                     KFPYNISDPDVRAAFEKKY"
BASE COUNT          449 a          704 c          725 g          357 t
ORIGIN      
        1 tggcaggcgg tcttgaaggc ctgggtcaag cggggcatcg agcgcggcgt cgatggctac
       61 atcgccaact acttctacaa gcacgattgc cactgcgagc attgtcagcg ggagttccgc
      121 gcttacatga gagggcggca ctcggccgaa gagttgaagg ccgcgtatag catcagcgat
      181 cttgcaactc ttaagtttga ggaaatggcc tattggcacg atccagctca gacaacgccg
      241 ctgaagctcg aaatgctccg ctgggcgcag atttccaaca agcaagtctt cgacaaggtc
      301 ttcgttgact atggccgctc gctgaagaag gacctgatcg tcgcgcaatg gaatcacctg
      361 ggcgactttg gccagatcag cggcgacgaa cgctgcctgc tgcccgcgga tttgtggggg
      421 aaaggcgaag actacctctg gtacagcacc ggcggggcgg cagcggcaac cgacatcgcc
      481 gcgggcaacc tcggcgaagg cacgctgcaa gcccgctaca tccgcggcat gttcgacgac
      541 aagccgttca cgctcggcaa gtacgagagc acacggaccc gcgtcaccat cgccgagctg
      601 gccgccaacg gcggcgcgcc gatggggttt tacacgcgct tcaccgatcc tgaggcgcga
      661 aaggagatcg tgcgctatta ccagttcctc aagcgctacg acgaaatcta tcgggccaat
      721 cgcgagcacg cggagttcac gctccacttc ccgcgcaagg aaatggcccg cgaggccgtc
      781 gcggccatcg aacgcgtcga gggattcaag tggctggcca aggccctgct cgacgagcac
      841 gtgctgtttg ccgttctttc cgacgagaat gctggccgct ggaactcgcc gccgctgcgg
      901 ctggaccggc cgccggacga tcctgcacgg acgcgcgagg gcatccacac cgcggtccag
      961 ggggcgatct tcagccggtt tgatgcgcct tcgaccgtgc gcatctcggc cagccggccg
     1021 gcacagggca atgagctcga cattcacttc gtgaactaca accgcaccga gccgcccaag
     1081 ggccgcgacg gtaagccgag cccgggcggc gggatcaagg acgagcagcc gatcgaagcg
     1141 ccgccgatcg cctgcgacgt ggtcgttccg gcgggattca cagccgcgag catcgaggct
     1201 atcacgcccg agcagccgga gccggtcgcg atccagttca ccgccgaggg cgatcgaatt
     1261 cgctttagtt tgcccaagtt tcttgtgtac agcgtggcga ggattcagtt gaaagcaaag
     1321 tagcgcagca gatcatcctt ggggcacatc atgtttcgcg cgcgtggcac gtggctcgcc
     1381 ggctttctct ggatcgctct tgggccggcg ctcgttaccg cccaggaatc gctctggatc
     1441 gaggccgaac acttggacgg cgtccgcggc tactgctggc cgatgggcaa gccggagatg
     1501 aaaaagaccg acggccactg gggcttgtcg ggacccggct gggccgccga gtggaatcaa
     1561 ggcggcgaaa gtggcttcct ctcgatcgcc accgccgcag acgacgacca ggccgtcgcc
     1621 acgaaaacaa tcgaagtccc cgcggccggc agctaccagg tctgggtccg ctacggcgat
     1681 tggcgcgaaa cgccggagag attcgcagtg cagatcgagc agccggggcg gccggtgtgg
     1741 agcggcagtt tcggggaacg ggcggtgatc gaagaagaca atgagatgaa gctctactgg
     1801 ggctgggcct ttgcctggga ctcgcggccg gccgatctcg ccaaaggacc ggcgacgctc
     1861 aagctcgttt ccaccacgaa gcagcagcag ccgcgacaga tcgattgcat cgtgctcacg
     1921 accgattcgg cttaccaacc ccggattaag gagcggccga agaaccacgc ctgggagctg
     1981 ctcgacagct accgcggccg tgtgccgccg ctggagccgc tggcccgccg cgtgcccgat
     2041 tggaagcagc cggcgacgta cgaatcgccc gcggcctgga agctgcacac gtttcgcgac
     2101 caggaattcc tgtacctgtg gaacgtgagc cacaccaacg cggcggagac ctggctcagc
     2161 gacaagccgg agcggatcaa gttcccgtac aacatcagcg atcccgacgt ccgcgcggcg
     2221 ttcgagaaaa aatac
//
LOCUS       DASRJB010000280         5687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_643159, whole genome shotgun sequence.
ACCESSION   DASRJB010000280 DASRJB010000000
VERSION     DASRJB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643159"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1997
                     /locus_tag="VFB80_07530"
     CDS             198..1997
                     /locus_tag="VFB80_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33653.1"
                     /translation="MDRTQLAALALGLFSAIDAAAQIPRDPFSGQTSSPPAAETAPRE
                     LPKPFVTRQSDVEIPFNVRGGTTPDSQPTAVRISVSWDQGRSWHFYDERRPEEGRFRF
                     RAKQDGEFWFATQTIDRAGRPDIPQPKWPQLRLVVDTQKPQLLVQAQVDSSGNVNVAL
                     SAADGSLNAASLKVEYQEAAGTGGPWEVVELKPSGGGPAQLTGQTTFRPNVTGRAINL
                     RAEVADGAGNIAYHSQRLSLIPPPPKPAAGIAAAAPAADPSATKWPSEPPALASEQVV
                     IPNLVANPFFNSGNPGRLASGPTSPNSGAPGPESLPPPQTPAESPLPPPADPLPLPGS
                     AAPQSLPEFGNDQPAPHLVPPARGPEVMPAPQGPGAETVQTPNGQRPRLTNSRRFSLE
                     YDVETVGPEGLQAVELWGTTDGGRSWVKWGADPDKQSPFDVEVNSEAIYGFRIVIVGR
                     NGLATSSPRPGEAADIWVGVDLTRPAARLTRAAYGQGAAAGKLDIRWEASDANLGSRP
                     ITLSLGERADGPFAPLAAGLPNSGQYLWEFDPRSPRQIYLRLEVRDEAGNIAIDQLNE
                     PIKVEGLEPKGRIRSFNPGPEPIDGAFRTPLFR"
     gene            complement(2165..5266)
                     /locus_tag="VFB80_07535"
     CDS             complement(2165..5266)
                     /locus_tag="VFB80_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZN33654.1"
                     /translation="MLTALIDLSLNNRFLVIALFCLLAIGGAYSATQIPIDAVPDMTN
                     TQVQVLTEAPGLSPIEVERLVTNRVENAMAGLPELTELRSTSKFGISAVTLVFHENTD
                     IYRARQLVNERITQVQGELLPGHGPPELGVLATALGEVLQFEVRSRGPGGRPVRDAMA
                     LRSILEWQIAPQLRQVPGITEINSIGGYYQTFEVQLDPNRMASQRIGLEEVLAALPRN
                     NASSGGGYIVHEDEQRFIRVDALLRDIAEIEQVVVKSSDEGASLRVKDVADVVVAPLT
                     RQGAATRDGRGEIVTGMVIMLLGENSRTVVHRAKERLREIQETLPADVKLEILYDRAD
                     LIQRTLHTVVHNLLEGGVLVIVVLLVLLGSWRAGLIVALAIPLAMLFASNVMLATGIT
                     ASLMSLGAIDFGLIVDSSVIMIENCIRRIAHNASGRSHWQVVRDAALEVRQPTMFGEL
                     IIAVVYLPILTLQGTEGKLFRPMALTVLFALAGSMLLSLTLMPVLAYAALPRRMNERE
                     VWLLRLVKRLYRPLVEWALAWPAATALAAVLTVAASLPVALRLGAEFMPKLDEGDILI
                     EANRLPSSTLEGSAAISRQIEELLLRFPEVKTVFCKTGRPEIANDLMGVQQTDVWVML
                     KPEKEWPRRKTRDELIEEMAAVLNGQVPGAAFVFTQPIEMRVDELVAGVKADVAVLLY
                     GDDLQILAEKGKEIERVLRGVRGAVDVKADYQANVSTLSIVPRRSQLARYGLDAQAIL
                     DAVTAVGGYQVGHIFEGRARYPLIVRYPLSWREDAQRLAQLPVHIHGEQIVPLGELAE
                     IVLEETPPAIEHEAGRRRTFVQANVRGRDVASFVQEAQQRVKSQVKLPTGYRVEWGGD
                     FENLRSASLRLAIITPLVLLLILLLLFTSLQSARLAALIFLAVPMAASGGIFALWLRG
                     MPFSISAGVGFIALFGVAVLNGLVWVSGAEHLRAAGRAPRDAAQETAETRLRPILMTA
                     LVASLGFLPMATSTTAGAEIQRPLATVVIGGLVTSTLLTALVIPAIYAWFAPRSPLPL
                     GEG"
     gene            complement(5299..>5687)
                     /locus_tag="VFB80_07540"
     CDS             complement(5299..>5687)
                     /locus_tag="VFB80_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN33655.1"
                     /translation="PAFDDAQLTAKVRFVGNQVSPETRSVPLVADLPNTDGRLKPGLF
                     VWALAPLGNTRPALVVPAGAILRHENQPFVFVPAGERTYRRVDVEVGLDAGDRVEILS
                     GLKAGDSVIDRGAFFLKSELLLEREE"
BASE COUNT         1001 a         1926 c         1856 g          904 t
ORIGIN      
        1 gcgcctgcgc ctccggctac gggttaaacg agcgacgccg ggccgcctgt caaactttgc
       61 ggcccgcaga ctttcgcgcg tttgcctgag cggtagtcgc ggcgtacccc catcaagccg
      121 cacgtctggt aagcccgata cagactgaaa gcgtcccccc ggcggcggac gctcttgcca
      181 gttgccaggg aaggcacatg gatcggacgc agcttgccgc gctggccctc ggcctatttt
      241 cggcgatcga cgccgccgcg cagattccgc gcgacccgtt cagcggacag acctcgtcgc
      301 cgcccgccgc cgagaccgcg ccgcgcgaac tgcccaagcc gttcgtcacg cggcaaagcg
      361 acgtggagat tccgttcaac gtccgcggcg gtacgactcc cgattcgcag ccaacggcgg
      421 tgcggatttc ggtctcctgg gaccagggcc gctcgtggca cttctacgac gagcgccgcc
      481 cggaagaagg ccgctttcgc ttccgcgcca agcaggacgg cgagttctgg ttcgcgacgc
      541 agacgatcga ccgcgccggc cgccccgaca ttccgcagcc caagtggccg cagctccgcc
      601 tggtcgtcga tacgcagaag ccgcagttgc tggtgcaggc ccaggtcgat tcttccggca
      661 acgtcaacgt cgcgctgagc gcggccgacg gcagcttgaa cgcggcctcg ctcaaggtcg
      721 aatatcagga agcggccggc acgggcgggc cgtgggaagt tgtcgagctc aagcccagcg
      781 gcggcgggcc cgcacagctc acggggcaga cgacctttcg tccgaacgtc accggccgcg
      841 cgatcaacct ccgggccgag gtcgccgacg gcgcgggcaa tatcgcctat cacagccagc
      901 ggctgtcgct gattccaccg ccccccaagc cggccgcggg aattgccgcc gccgcccccg
      961 cggccgatcc atcggcgacg aagtggccca gcgagccgcc ggcgctagcg agcgagcagg
     1021 tcgtcattcc caacctcgtc gccaatccgt tcttcaattc cggcaatccc ggccggctgg
     1081 caagcggccc gacgtcgccc aattcgggcg cacctggacc ggaatcactt cctccgccgc
     1141 agactccagc cgaatcgccg ctgcccccgc ccgccgatcc gttgcctttg ccgggcagcg
     1201 ccgcgccgca atcgctgccg gaatttggca acgatcagcc agcgcctcat ctcgttccac
     1261 cggccagggg cccagaagtc atgcccgcac cacaagggcc tggagccgag acggtccaaa
     1321 cgcctaatgg ccagcgcccg cggctgacga actcgcggcg cttcagcctg gaatacgacg
     1381 tcgaaaccgt gggcccggaa ggcctgcagg cggtcgagtt gtggggcacg accgacggcg
     1441 gccgcagctg ggtcaagtgg ggcgctgatc ccgacaaaca aagcccgttc gacgtcgaag
     1501 tgaacagtga agcgatctat ggcttccgga tcgtgatcgt cgggcgcaac ggcctggcaa
     1561 cgagcagccc gcggccgggc gaagcggcgg acatctgggt cggcgtcgac ctgacgcggc
     1621 ccgcggcccg cctgaccaga gcagcctatg gtcagggcgc agcggcgggg aaactcgata
     1681 ttcgctggga ggcctccgac gccaatctcg gcagccggcc gatcacgctc tcgctcggcg
     1741 agcgggccga cgggccgttc gctcccctcg ccgccggcct gcctaacagc gggcaatacc
     1801 tgtgggaatt cgatccccgc agcccacggc aaatctacct gcggctggaa gtccgggacg
     1861 aagcgggcaa catcgccatc gaccagctca acgagccaat caaggtcgag gggctggagc
     1921 ccaagggccg catccgtagt ttcaatcccg gccccgagcc gatcgacggg gctttccgca
     1981 cgccgctgtt tcggtaggtc ccctctgctc ttctccctct gggagaaggc cggggagggc
     2041 aggcaacggg attaagataa ggattacgat taagattagg agtttgtgat gctaatccta
     2101 atcctaatct taatcgtcat cctaatcttt gccgtgttct tggtcgttga cgacgaccct
     2161 caccctaacc ctctcccaaa gggagagggg accgcggtgc gaaccacgcg tagatcgcgg
     2221 gaatcactag cgccgtcagc agcgtcgaag tcaccagccc gccgatgacg accgtggcca
     2281 gcggccgttg aatctctgcg ccggcagtcg tcgaagtggc catcggcagg aagccgaggc
     2341 tggcgacgag ggcagtcatc aggatcggac gcaggcgcgt ctcggccgtc tcttgggcag
     2401 catcgcgcgg ggcgcggcct gcggctcgca ggtgctccgc gccgctcacc cacaccaggc
     2461 cgttgagcac ggcgacgcca aacagggcta tgaatccgac gcccgcggag atgctgaaag
     2521 gcatgccgcg gagccagagc gcgaagatgc cgcccgaggc cgccatcggc acggccagga
     2581 aaatcagtgc cgccagccgg gctgattgga ggctcgtgaa gagcagcagc aagatgagca
     2641 ggagcaccag cggcgtgatg atcgccaggc gcaggctcgc cgagcggagg ttctcgaagt
     2701 cgccgcccca ctcgacccga tagccagtcg gaagcttgac ctgactcttg acgcgctgct
     2761 gggcctcctg gacaaagctg gccacgtcgc ggccgcggac gttggcctgc acgaacgtgc
     2821 ggcggcggcc cgcctcgtgc tcgatggccg gcggcgtttc ttcgagcacg atctcggcca
     2881 gttcccccag cggcacgatc tgctcgccgt ggatgtggac cggcagttgg gccagccgct
     2941 gcgcgtcttc gcgccaggag agcggatagc gcacgatgag cgggtagcgg gcgcggcctt
     3001 cgaagatgtg gccgacctgg tagccgccca ccgcagtgac cgcatcgagg atcgcctgcg
     3061 cgtcgaggcc gtagcgggcc aattgcgagc gccgcggcac gatcgagaga gtgctcacgt
     3121 tggcctggta atcggctttg acatccacgg ccccgcggac gccgcgcagc acgcgctcga
     3181 tttccttccc tttctccgcc aggatctgca agtcgtcgcc gtacagcagc acggctacat
     3241 ccgctttgac gcccgccacc agttcatcga cgcgcatctc gatcggctgc gtgaaaacga
     3301 acgccgcgcc gggaacctgg ccattcagca cggccgccat ttcctcgatc aattcgtcgc
     3361 gggtcttacg gcgcggccac tctttttcgg gcttgagcat gacccagaca tccgtttgct
     3421 gtacccccat caagtcgttg gcgatctccg gccggcccgt cttgcaaaag acggttttga
     3481 cttccgggaa tctgagcagc agctcctcga tctgccgcga aatcgccgcc gagccttcca
     3541 gcgtcgagct gggcaggcga ttggcttcga tcaagatgtc gccttcatcg agcttcggca
     3601 tgaactcggc gcccagtcgc agcgccacgg gcaggctggc ggcgaccgtc agcaccgcgg
     3661 ccagtgccgt cgccgccggc caggccaagg cccattcgac cagcggccgg tagagccgct
     3721 tcaccagccg cagcagccag acttcgcgct cgttcatccg ccgcggaagc gccgcatagg
     3781 ccagcacggg catcagcgtc agcgaaagca gcatcgagcc cgccagcgcg aacagcacgg
     3841 tcaaagccat ggggcgaaag agtttgccct cagtgccttg cagcgtgagg atcggcagat
     3901 aaacgacggc gatgatcaat tcgccgaaca tcgtgggctg gcggacttcg agggcggcgt
     3961 cgcggacgac ctgccagtgc gatcgtcccg acgcgttgtg cgcaatgcgg cggatgcagt
     4021 tctcgatcat gatgaccgag ctatcgacga tcaggccgaa gtcgatcgcc ccgaggctca
     4081 tcaggctggc ggtgattccg gtcgccagca tcacgttgga ggcaaacagc atcgccagcg
     4141 gaatggccag ggccacgatc aggccggccc gccaactgcc caagagcaca agtagcacga
     4201 caatcaccag cacgccgccc tcaagcaggt tgtgcaccac cgtgtgcaac gtccgctgga
     4261 tcaagtcggc ccgatcgtag aggatttcca gcttgacatc cgccggcagc gtttcctgaa
     4321 tctcccgcag ccgctctttc gcgcggtgca ccaccgtgcg gctgttctcg ccgaggagca
     4381 tgatgaccat gccggtgacg atctcaccgc ggccgtcgcg cgtggctgcg ccctgccggg
     4441 taagcggagc gacgaccacg tcggcgacgt ccttgacgcg cagggacgcg ccctcgtcgc
     4501 tgctcttgac cacgacttgc tcaatctccg caatgtcgcg cagcagggca tcgacgcgga
     4561 tgaaccgctg ctcgtcctcg tggacgatgt agccgccgcc ggagctggcg ttgttccggg
     4621 gcagggcggc gagcacttcc tccagtccga tccgctggct ggccatgcga ttgggatcga
     4681 gctgcacttc gaacgtctgg taatagccgc cgatcgagtt gatctccgtg atgccgggga
     4741 cttgccggag ctgcggggcg atttgccatt cgaggatcga ccgcaaggcc atcgcgtcgc
     4801 ggaccggccg cccgccgggc ccgcggctgc ggacctcgaa ctgcaacacc tcgccgagcg
     4861 cggtcgccag cacgcccagc tccggcgggc catggccggg cagcagttcg ccctggacct
     4921 gcgtgatgcg ctcgttgacg agctgccggg cgcggtagat gtcggtgttt tcgtggaata
     4981 cgagcgtgac cgcagagatg ccgaacttcg atgtgctgcg cagctcggtc agctcgggca
     5041 gtcccgccat cgcattctca acgcggttgg tgacgagccg ttcgacctcg atcggcgaca
     5101 ggcccggcgc ctcggtcagc acttgcacct gcgtgttggt catgtccggc accgcgtcga
     5161 tcggaatctg cgtggcgcta tacgcgccgc cgatcgccag caagcagaac agcgcaatca
     5221 ccaggaagcg gttgttgagc gacagatcga tgagggcggt aagcacgggc gtcgggagtc
     5281 aggagtcggg agtcggagtc attcttctcg ttcgagcagc agttccgatt tcaggaagaa
     5341 cgcgccgcga tcgatgacgg agtcgccggc tttcaggccg gaaagaattt cgacgcgatc
     5401 gccagcgtcg agtccgactt cgacatcgac gcgccggtag gtgcgttcgc cggcgggcac
     5461 gaatacgaac ggctgattct cgtgccgcag gatcgcgccc gctgggacga ccagcgcggg
     5521 ccgggtgttg cctagtggcg ccagtgccca gacgaataag cccggcttca gcctgccgtc
     5581 ggtgttgggc agatcggcaa ccagcggcac ggagcgggtc tcgggagaga cctgattgcc
     5641 gacgaaccgc actttggccg tcaattgggc gtcgtcgaag gcaggaa
//
LOCUS       DASRJB010000281         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1209165, whole genome shotgun sequence.
ACCESSION   DASRJB010000281 DASRJB010000000
VERSION     DASRJB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209165"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFB80_07545"
     CDS             <1..289
                     /locus_tag="VFB80_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN33656.1"
                     /translation="DLVGLSHRLTHKPRELSGGEMQRTAIARALIAQPKLLLADEPTG
                     NLDSKTGGEILTLLKKLNREQGLTIVMVTHDQAIASKADRTVRLVEGKVAA"
     gene            408..1010
                     /locus_tag="VFB80_07550"
     CDS             408..1010
                     /locus_tag="VFB80_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33657.1"
                     /translation="MLAAQLRSGIAHVGQALRTPEEFAVAWNDGSVRYRWWVWLSLMA
                     TAILGTTTYGMTMGLLGGTGDVLYKGLACTLAAGLAWGIPLPALYILNSLTGSRLPAS
                     STLLAALVTTSWGGLAMLASIPINWFFSVAVPHPAFILAVNLTVFAGVGVAMIDVFHR
                     VMRSLEPHREVPPTWWLLLVGAIGGELFYFFGLFQFTVTS"
     gene            1022..1882
                     /locus_tag="VFB80_07555"
     CDS             1022..1882
                     /locus_tag="VFB80_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33658.1"
                     /translation="MTTVQVSSRLHRIAEANSPCDGGPELEISDEPAGEEPLRTGLID
                     LVELILKRRESLERLIRQPALQAQLVPKFLTISLLGFVFFGVALALVFTAAGMWPRLF
                     AIDDVVAGEATRIAAFEQFAPAGRLGRWLDGSAAALIAAYALGLIAASGICLPSLYFY
                     SLLAGVRMSMLDVVVQTLKSKATAAVALVGILPIYAALGLAIVIFPVPASLVEATFWL
                     GLILPFLAGLFGTYSLYRGLAGYADTLPAERRARRATFLQRLVVSWAACYTAVTPVMI
                     HTLWVFLARA"
     gene            1909..2574
                     /locus_tag="VFB80_07560"
     CDS             1909..2574
                     /locus_tag="VFB80_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZN33659.1"
                     /translation="MRLLNRLADIVSANLSELIERYEDPELLLKQAVREMDESLRAAL
                     ENAVKVVAHEKLLARQLAEEEAAATAWHKQAAAAVQRGDDAAARAALRQKRERESVVV
                     ALAGQLAEATAAGQTLRREIELLRLRTDEAKRKLTLLAARQRAAEARQRLLREFNAAP
                     IGDDAFAKFERMCRKVEQTEAEAEALAELAGRPQPDRCTDKTGADDGVESELAELKAA
                     CGA"
     gene            2586..3269
                     /locus_tag="VFB80_07565"
     CDS             2586..3269
                     /locus_tag="VFB80_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33660.1"
                     /translation="MNLATTLLFYGVIGAAVAVAVYLSFEAVEKRQRWLAALTAVAFW
                     PLYLPLLLRPAAPRADSVPTETKPPPYDELTAAIQQVEAELDLALQSLDGWSDAVLAR
                     EQHRFAELRAAWHAQAEKIRQLDLLLAQPEYASDLHGEPQPAAHARDRARQANIARLR
                     ALRDKFHAELTGTLAWVRELVTMIHLAKYTGAPASRAEELVMQIATSVEGLSEVAAWQ
                     GAESAGNLR"
     gene            3395..3880
                     /locus_tag="VFB80_07570"
     CDS             3395..3880
                     /locus_tag="VFB80_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33661.1"
                     /translation="MLLLTTAVGAMVAVFVAYYRQSVPLVKSRLYEEFGTTEYLRAVG
                     APLHSRPPQLLVTNGTTHTHEGSDANCRVSERTLGLPRRSRPEFMRRLHQDAVGLIKT
                     DHRSLVGELRNGQTKDDLQGFTYRYRGGKTRGVLHVERVDLSDEEMHLSIVIYEHADE
                     R"
     gene            3858..4421
                     /locus_tag="VFB80_07575"
     CDS             3858..4421
                     /locus_tag="VFB80_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33662.1"
                     /translation="MSTRTSAEQTATSESAPARRRGAWWRFSIRELLLLTAAVAAFLA
                     WAGLLYQRSLPYQRTTIPDTIGNFQDVRTICAKIGHRPASYSSGGGGSSNMYATIRSY
                     DCQVDLPAKLRGQFMADYREHIRATLAKLADRVGGAGSISDGRGLQGFSYEYAEGRVH
                     GTVVVRSSSRDDGLSLFIFVHEYDSRR"
BASE COUNT          715 a         1509 c         1478 g          765 t
ORIGIN      
        1 ggacctcgtc ggcctctcgc accgcctgac gcacaagccg cgcgagctct ccggcggcga
       61 gatgcagcgg accgccatcg cccgggccct gatcgcccag cccaagctgc tcctcgccga
      121 cgagcccacc ggcaacctcg actcgaaaac cggtggcgaa atcctcactc tcttgaaaaa
      181 gctcaatcgc gagcaggggc tgaccatcgt catggtcacc cacgaccagg cgatcgccag
      241 caaagccgac cgcaccgtgc ggctagtcga aggcaaggtc gcggcctagc gtccatattt
      301 ggcccgttaa tccgccagca agcctgcggt tgaaactccg ccgcggattc agactgcccc
      361 ttctgcattc tgaattccgc attctgcatt tcctcgcgga ggcggttatg ctcgctgccc
      421 agctgcggtc tggaattgcg cacgtcgggc aagccctgcg gacgccggaa gagttcgccg
      481 tggcctggaa cgacggctcg gtgcgctatc gctggtgggt ctggctctcg ctaatggcca
      541 cggcgatcct cggcaccacg acctacggca tgacgatggg cctgttgggc ggcacgggcg
      601 acgtgctcta caaaggcctg gcctgcacgc tcgccgccgg tctggcgtgg ggcattccgc
      661 tccccgcgct ctacatcctc aacagcctca ccggctcgcg gctgccggcc agcagcacgc
      721 tgctggcggc gctggtcacg accagctggg gcggcctggc gatgctggcc tcgattccga
      781 tcaactggtt cttctcggtc gccgtgccgc acccggcgtt cattctcgcc gtcaacctga
      841 ccgtctttgc cggcgtgggc gtggcgatga tcgacgtctt tcaccgcgtg atgcggagcc
      901 tggagccgca ccgcgaagtg ccgccgacct ggtggctgtt gctcgtcggc gcgatcggcg
      961 gcgagctgtt ctatttcttc ggcctgttcc aattcaccgt gaccagctag aggcgcctgc
     1021 catgacaacg gtccaagtga gcagccggct gcaccgcatt gcggaagcca attcgccgtg
     1081 cgacggtggt cccgagctcg aaatctccga cgagccggcc ggcgaagagc cgctgcggac
     1141 cgggctgatc gacctggtgg agctgatcct caagcgccgc gagagcctgg agcggctgat
     1201 ccgccagccg gcgctgcaag cccagctcgt gcccaagttc ctgacgattt cgctgctcgg
     1261 attcgtgttc ttcggcgtgg cgctggcgct ggtcttcacg gcggccggaa tgtggccgcg
     1321 gctgtttgcg atcgacgacg tcgtcgcggg cgaggcgacg cggatcgccg cgttcgagca
     1381 gttcgcccct gccggtcggc tcggccgctg gctcgatggc tcagccgcgg cgctgatcgc
     1441 cgcgtatgcg ctggggctaa ttgcggcatc gggcatttgc ctgccgagcc tgtacttcta
     1501 cagcctgctg gccggtgtgc ggatgtcgat gctcgacgtc gtcgtacaga cgctcaaatc
     1561 gaaagccact gccgctgtcg cgctggtcgg cattctgccg atctatgcgg ccctaggctt
     1621 ggcgatcgtg atcttcccgg tccccgcgtc gctggtggaa gcgacgttct ggctgggatt
     1681 gatcctgccg tttctggccg gcctgttcgg cacgtactcg ctctatcgcg ggctggccgg
     1741 atacgccgac acgctgccgg ccgagcgccg cgcccgccgg gcgacttttc tgcagcggct
     1801 cgtcgtctcg tgggcggcgt gctacacggc agtgacgccc gtgatgatcc acacgctgtg
     1861 ggtcttcctg gcacgcgcct gattcgacga accaacctgg agaaacccat gcgactgctc
     1921 aaccgcctgg ccgacatcgt ttccgccaat ctgagtgagc tgatcgagcg gtatgaagac
     1981 ccggagctgc tcctcaagca ggccgtccgc gagatggacg agtctctgcg ggccgcgctc
     2041 gagaacgccg tcaaggtcgt cgcccacgag aagctgctcg cgcggcaact ggccgaggaa
     2101 gaagccgcgg cgaccgcctg gcacaagcag gcggccgccg ccgtgcagcg gggcgacgac
     2161 gctgcggccc gcgcggcctt gcggcagaag cgcgagcgcg agtcggtcgt cgtcgcgctg
     2221 gccgggcagc tggccgaagc cactgccgcg ggacagacgc tccgccgcga gatcgaactg
     2281 ctgcggctgc ggaccgacga ggccaaacgg aagctcacgc tgctcgccgc ccgccagcgg
     2341 gccgccgagg ctcgccagcg gctgctgcgc gaattcaacg ccgccccgat cggcgacgat
     2401 gcttttgcca agtttgagcg gatgtgccgc aaggtcgaac aaacggaggc ggaagccgag
     2461 gccttggcgg aactggccgg caggccgcag ccggatcgct gcaccgacaa aaccggggca
     2521 gacgacggcg tcgagtcgga gctggccgag ttgaaggcag cctgcggcgc ttgacggagg
     2581 gccgcgtgaa cctcgccacg acactgctgt tctacggcgt gatcggcgcg gccgtcgccg
     2641 tggccgtgta cctgtcgttc gaggcagtgg aaaagcgcca gcgatggctg gcggcgctga
     2701 ctgccgtcgc gttctggccg ctctatttgc cgctgctcct gcggccggct gctccgcgcg
     2761 ctgattcggt gcccaccgaa acgaagccgc cgccttacga cgagctcact gccgccatcc
     2821 agcaggtcga ggccgaactc gatctggccc tgcaaagtct cgatggctgg tccgacgcgg
     2881 tcctggcccg cgagcagcat cgcttcgccg agctgcgggc cgcctggcac gcccaggccg
     2941 agaaaatccg ccagctcgac ctcctgctgg ctcagccgga atatgcctct gatttgcatg
     3001 gcgaaccaca gccagcggca cacgctcgcg atcgcgcgcg gcaggcgaac atcgcccggc
     3061 tgcgggccct ccgcgacaag ttccacgccg agctgaccgg cacgctggcc tgggtccgcg
     3121 agctggtgac gatgatccac ctggcgaagt acacaggcgc tcccgcctcg cgggccgagg
     3181 agctggtaat gcaaatcgcg accagcgtcg aaggcctctc ggaagtcgcc gcctggcagg
     3241 gggcagaatc ggccggcaat ctgcgatagg cttgaagcac ttccttcgca gcccgccgag
     3301 actcctgctc gtgaacgacc gctccgattc tctgcaggcc ggcccagcgc tcccggcgag
     3361 ggccaggccc tggtggcggt tctcgattcg ggaaatgctg ctgctgacta cggcggtcgg
     3421 cgcgatggtg gcggtcttcg tggcctacta tcggcaaagc gtgccgctgg tgaagtcgcg
     3481 gctgtacgag gagttcggca cgaccgaata tctgcgggcg gtgggtgcgc cgttgcattc
     3541 gcggccgccg caactcctag ttaccaatgg caccacgcat acccatgagg gttcggacgc
     3601 gaactgccgg gtatccgaaa gaacgctcgg ccttccccgc cggtcccgcc cagagttcat
     3661 gcgcaggttg caccaggatg ccgttggcct gatcaagacg gaccaccggt cgctggtcgg
     3721 cgaactccgc aacggacaaa cgaaagacga cctgcaaggc ttcacctatc gataccgggg
     3781 cggcaagacg cgaggcgttc ttcacgtcga gcgcgtggac ctctccgacg aggaaatgca
     3841 cctgagcatc gtgatctatg agcacgcgga cgagcgctga gcagactgca acttccgaga
     3901 gcgcccctgc ccgccgaaga ggcgcttggt ggcggttttc gatccgcgag ctgctcttgc
     3961 tgacggcagc ggtggcggcg tttctggcgt gggccggctt gctctatcag cggagcttgc
     4021 cttaccagcg cacgacgatt ccggacacca tcggcaactt tcaggacgtc cggacgatct
     4081 gcgcgaagat cgggcatcgc ccggcgtcgt actcgtcggg cggcggtggc tcgtcgaaca
     4141 tgtacgccac gatccgttcg tacgattgcc aggtcgatct gccggcgaag ctgcggggcc
     4201 aattcatggc cgactaccgc gaacacattc gcgccacgct cgccaagctg gccgatcgcg
     4261 tcggcggagc cggctcgata agcgacggcc gcggcctgca gggctttagt tacgagtacg
     4321 ccgagggccg cgtccacgga accgtcgtcg tccgctcgtc cagccgcgac gacggtctgt
     4381 cgctgttcat cttcgtccac gaatacgaca gccggcgata acaacccgaa gcgtaagcgc
     4441 gtaagcgagg ggttttctaa gttcggg
//
LOCUS       DASRJB010000282         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1492726, whole genome shotgun sequence.
ACCESSION   DASRJB010000282 DASRJB010000000
VERSION     DASRJB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492726"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFB80_07580"
     CDS             <1..602
                     /locus_tag="VFB80_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33663.1"
                     /translation="QAVGTEEADGISVSRPGKDEFSVRATFIPNSSRKYPAEGVTSIA
                     MILCGGDDQATIAGTIDLPTYITAEEGNDDINGGGGPNVLLGGDGDDRVTGGSNHDIL
                     VGGLGTDRIVGNGGDDILIAGTLGGAADPIDQLDMLLALVTEWELDRDRDRLRPKLLV
                     GGDHDADMLTGSSDSDWFFFDFDEDTATDLKNELAESIG"
     gene            1150..3219
                     /locus_tag="VFB80_07585"
     CDS             1150..3219
                     /locus_tag="VFB80_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN33664.1"
                     /translation="MASGQVSSETIEKTKQQIRGLVGEIAQLAKSEMEPEEFYSAFLQ
                     RVVQALAAKGGAIWVLGEAKKPELAYQIDISPTLLDKESDEASKHFRLLDYIVATGTP
                     QLVPPLSGAGDERMGGNPTRHLLVIHPLGHDSQVEGLVEIFQRSDTQPQTQRGYLQFM
                     KQMCELTAEWFKNRKLRDLGDRHSLWSQADQFARQVHESLDVRETAYTIVNEGRRLMG
                     CDRVTVGILKGGRCVVEAVSGQDTLDNRSNVVTLLGKLATRVVASGEPLWYAGATDDM
                     PPQIEEAIEEYVDQSYTKSLAVIPLRQPKPTEAAQQQQQQQPGGEGTPVENAHTGQIV
                     GALIIEQIESEIPRDVLAPRLDLVYEHSARALTNALDHNSLFLMPVWRTIGKSRWLVR
                     GRQLPKTVLIAGGLLVLLVAGLAIPGDFDMRAKGTLQPVVRQDLFAPSAGDVIGVLKG
                     SGDTVQAGETVIQLHNAELKLKLNEVEGRLSGSLAQYQSIDKQLLNSQAMTAAEKLKI
                     QADQANLTIQLQSLRDQLALLKKREEQLTIKSPIAGRVTTWDAKRLLQNRPVETGQVL
                     MTIAAEDTAYELEINMPERHMDHVRRAREVLKRSDPNADLGVDFILMTDPGQNHTGKV
                     VHINPTAEPHEDQGNIVRIRVQPDQELTSPRPGATVTADVHCGRAPLLWTKLHQAWEW
                     VEANLLF"
BASE COUNT          680 a         1132 c         1079 g          538 t
ORIGIN      
        1 tgcaggccgt cggcaccgaa gaagccgacg gaatttcggt cagtcgcccg ggcaaggacg
       61 agttttcggt ccgcgccaca ttcattccca attcttcgcg gaagtacccc gcagagggtg
      121 tgacttcgat cgccatgatc ctctgcggcg gcgacgatca ggcgaccatc gccggcacca
      181 tcgatctgcc gacctacatc acggctgagg agggcaacga cgatatcaac ggcggcggcg
      241 ggccgaatgt cttgctgggc ggcgacgggg acgaccgcgt caccggcggc agcaaccacg
      301 atattctcgt gggcggcttg ggcacggacc ggatcgtcgg caacggcggc gatgacattc
      361 tcatcgccgg cacgctgggc ggagcggccg accccatcga tcagctcgac atgctcttgg
      421 cgctggtgac cgagtgggag ctggatcgcg accgcgatcg cctgcggccc aagctgctcg
      481 tcggcggcga tcacgacgcc gacatgctca ccggcagctc cgactccgac tggttcttct
      541 tcgacttcga tgaggacacc gccacggacc tcaagaacga gctggccgaa agcatcggct
      601 gattcgcacg gtgggacgaa gggagttgtg aggtcccgcc cgcgggcggg acctcgcctg
      661 caacatgccg ttgagtgcgg gcaacagggc cgggccgcta gcgccgcaat tgaagaatcc
      721 acaatgggct ggcccatggt gcggccgatc cgatcgctcc accgctaggg agtttgccgg
      781 cggaattgcc gcgattattg tccccaccgg caatctccgg cctggatttc caagtccgcc
      841 agagccaagg tacggcgttt gcgtcagacc ctgccgccac tggcccggct gccaattcgg
      901 cgagcatgcc agcagacatt cgccagcttt gactaatcgc tataggccac ccgattgcat
      961 ccgtattggg gcaccgcgcg gcgaattgcc cgcgcgactt aaaccaaaac gttgtaaggt
     1021 atttacgtca cgcgtagatt gcagattgcc gcctgaacgg ccccgcggcg cgtcccctac
     1081 aaccggcacc tggaagcctg ccgctcgcaa ctaaccccca gcgatcaatc ccaccgaaaa
     1141 ggcgcggcta tggcttccgg acaggtcagc tccgagacca tcgaaaagac caagcagcag
     1201 attcgcggcc tggtcggcga aatcgcccag ctcgccaaga gcgagatgga gccggaggag
     1261 ttttactcgg ccttcctgca gcgcgtggtg caggccctgg ccgccaaggg cggagcgatc
     1321 tgggtgctcg gcgaagccaa gaagcccgag ctggcctatc agatcgacat cagcccgacg
     1381 ctgctcgaca aggaatcgga cgaggccagc aagcacttcc gcctgctcga ttacatcgta
     1441 gccaccggca cgccgcagct cgtgccgccg ctctcgggag cgggagacga gcggatgggc
     1501 ggcaacccga cgcggcacct gctcgtcatt catccgctag ggcacgacag ccaggtcgaa
     1561 ggcctcgtcg aaatcttcca gcggtccgac acgcagcctc agacgcagcg cggctatctg
     1621 caattcatga agcagatgtg cgagctgacc gcggagtggt tcaagaaccg caagctccgc
     1681 gatctcggcg accgccactc gctctggtcg caggcggatc aattcgcccg ccaggtccac
     1741 gaaagcctcg acgtccgcga aaccgcctac acgatcgtca acgaaggccg ccggctcatg
     1801 ggctgcgacc gcgtgacggt gggcattctc aagggcggca ggtgcgtggt cgaggcggtc
     1861 agcgggcagg acacgctcga caaccgcagc aacgtcgtca cgctgctggg caagctggcg
     1921 acgcgggtcg tcgcctcggg cgagccgctg tggtacgccg gcgcgaccga cgacatgccg
     1981 ccgcagatcg aagaggcgat cgaggaatac gtcgatcagt cgtacaccaa gtcgctggcg
     2041 gttatcccgc tgcggcagcc caagcccacg gaagctgctc agcagcaaca gcaacagcaa
     2101 cccggcggcg aaggtacgcc cgtcgagaac gcccacaccg gccaaatcgt cggcgcactc
     2161 atcatcgagc agatcgaaag cgaaattccc cgcgacgtgc tggccccgcg gctggacctg
     2221 gtctatgagc acagcgcccg ggccctgacc aacgccctgg accacaacag cctgtttctg
     2281 atgccggtct ggcggacgat cggcaagtcg cggtggctgg tgcggggccg ccagctgccc
     2341 aagaccgtgc tgatcgccgg cgggctgctc gtgctgctcg tcgcgggcct ggccattccg
     2401 ggcgacttcg acatgcgggc caaggggacg ctgcagccgg tggtccgcca ggacctgttc
     2461 gccccgtctg ccggcgacgt gatcggcgtc ctcaagggca gcggcgacac agtgcaggcc
     2521 ggtgaaacgg tcatccagct ccacaacgcg gagctgaagc tcaagctcaa tgaagtcgaa
     2581 ggccgtctca gtggatcgct cgctcagtat cagtcgattg acaagcagct gctcaacagc
     2641 caggccatga ccgcggccga aaaattgaag attcaggcgg accaggccaa tctgacgatc
     2701 cagctgcaga gcctccgaga ccagctggcg ctcctcaaga agcgcgagga gcagctcacg
     2761 atcaagagtc cgatcgccgg ccgcgtgacg acttgggacg ccaagcggct gctgcagaac
     2821 cgccccgtcg agacgggcca ggtgttgatg acgatcgccg ccgaagacac ggcttacgag
     2881 ctggagatca atatgcccga gcggcatatg gaccacgtcc ggcgggcccg ggaggtcctg
     2941 aaacggtccg atccaaacgc cgacctgggc gtcgatttca tcctgatgac cgaccccggc
     3001 cagaaccaca ccggcaaggt cgtccacatc aatcccaccg ccgagccgca cgaggaccag
     3061 ggcaatatcg tgcggattcg cgtgcagccc gaccaagagc tgacgagccc gcgccccggc
     3121 gcgaccgtca ccgccgacgt ccattgcggc cgcgccccgc tgctctggac gaagctgcac
     3181 caggcgtggg agtgggtcga ggccaatctg ttgttctgat ttcagattta ggatttcaga
     3241 tttcagattt gagtctttgc aatctgaaat ctgaaatgat ccaggaggag ttccgatgtt
     3301 tcgcaaatcc ctcatcgtcg tttccctcgc cctcctggcg ctcgtcgtcg ccagtgctca
     3361 cagccagagc ccgtcggcca cgacgggaaa gaccatcgcg gttcaggacg ccttggtcac
     3421 cgggagcga
//
LOCUS       DASRJB010000283         6317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1492921, whole genome shotgun sequence.
ACCESSION   DASRJB010000283 DASRJB010000000
VERSION     DASRJB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492921"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFB80_07590"
     CDS             <1..341
                     /locus_tag="VFB80_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33665.1"
                     /translation="RGMLDETLVIWGGEFGRTPMAQDGKGEQLGRDHHMKGFSIWLAG
                     GGIKPGFTFGNTDDLGYHAAENIVQVHDLHATALYLLGLDHERFTYRYQGRDFRLTDV
                     SGRVVSEILA"
     gene            complement(423..1190)
                     /locus_tag="VFB80_07595"
     CDS             complement(423..1190)
                     /locus_tag="VFB80_07595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZN33666.1"
                     /translation="MSGLTQLLRRVTGGRWPRRRAMRDGRPHFDCWEQLGIAPGVTAD
                     TSQSRAADELHIDQFRAAPCYLNRLDHLHHALELAPADGYILEFGVYRGGSLNWLARW
                     AEQRRQPRVFGFDSFEGLPEAWVRTKSGESYARGHFALERLPAVLPNALLVPGFFDAT
                     LPGWRAAHAGPVALLHNDSDLYCSTIDTLTQLNDRIVPGTIIVFDELCDWRNSGVYDA
                     WEEGEWRALREWMAAHDRRIAVLSRNQEFAAAIRVVR"
     gene            complement(1215..1919)
                     /locus_tag="VFB80_07600"
     CDS             complement(1215..1919)
                     /locus_tag="VFB80_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN33667.1"
                     /translation="MPRAHTPASRPLMAAEALEKLLGEYDFTTVLDVGCGPGRHLERF
                     RSSGKTASGIDIVGLAEGVIVADYLRHEFQRPFDCLWLSHVLEHQLNVNFFLRKVFAD
                     LREGGVLAVTVPPLKHAIVGGHVTLWNAGLVLYNLVLAGFDCRQARVKQYGYNISVVT
                     PKIPAAIPYRELHYDEGDIDLLAPFFPQHPRLPIRQGFCGDIRQLQWDGDELVLAPPR
                     SIARRIRGFFSGRLAG"
     gene            complement(1938..2966)
                     /locus_tag="VFB80_07605"
     CDS             complement(1938..2966)
                     /locus_tag="VFB80_07605"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33668.1"
                     /translation="MSTILVEARRDPQPGEPLDLIAQTCRSLGHDVFRWRGPQSGRVP
                     HWRYLFRCDLAILWNGLNPKYDRAMATLRRRGTPLIFTELGWWPQRGHIQIDRAGINA
                     NASWAAEPLTSRPLTPLPVREQGDLLVVLQLDRDTQITRLSPWFANMQRFMEFICQSS
                     ALSVRVRHHPRERPPEEFIERVRQLGGTWDESPTLRAAMSGARALACVNSSCGVEALG
                     NGLPVLCYGKAIYRHPGAVYCLDDDPAATRAATKTLADGRCDLFREPAAEVVSRILSH
                     QWPVAEIPRQLPPLVEEALAGAARAEPRRASGWAGLAANAWRRASDGVVRWMRDLPPK
                     VLPARPVF"
     gene            complement(3084..3488)
                     /locus_tag="VFB80_07610"
     CDS             complement(3084..3488)
                     /locus_tag="VFB80_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN33669.1"
                     /translation="MHRAIWLIASVLFAGCGPGLVPVEGRVLLEDQPLADAQVLFLPK
                     SGGRPATGKTNAEGQFTLTTNRPSDGVRPGEYQVAVTALQVLYVRPTDGSEYAEQKVW
                     IAPERYSKPAESGLAATISPEANRPTFELRAK"
     gene            complement(3520..4524)
                     /locus_tag="VFB80_07615"
     CDS             complement(3520..4524)
                     /locus_tag="VFB80_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33670.1"
                     /translation="MGNRAAFTLVELLVVVAIIGLLVALLLPAVQAAREAARRMQCAN
                     HLKQLALALHGYHDTYQIFPFGSWAAWDSSNPPPAPAPRTESKGSMIHLVLPYYEQQN
                     TLSLIDFSKVIIEEQTAPVHFNNVIRKIRIKALVCPSSDYQGLHPTNRNALCSYAGSA
                     GPNALSASGNVRGNCTCYHPYNPFAIAKVPHLAASGPFGRHNSTPGASFPRRVATRIS
                     DVQDGLSATIFIGEMRPRCSSVANAGWADSNNGSGVMTTIVPINYDSCGPRAQWQTVD
                     GCRTDCNDTVSLGFKSLHAGGAQFCFGDGGVRFLNQSIDHWTYQYLGGVADGQPAAVP
                     "
     gene            4653..5384
                     /locus_tag="VFB80_07620"
     CDS             4653..5384
                     /locus_tag="VFB80_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN33671.1"
                     /translation="MKSPSSLAAVAFALLAASAGCRMVSTGRNVDGVRYFQQGNYPVA
                     IQRFQSAMTVDSRNPDSHYNLAAVKHREGLINRDQNSLTQAESLYQQALAYDPNHVDA
                     YRGLAVLLTETGRTSDAFALLNRWATTNPGSADARVEIARLHEEYNQPQQAEASLHSA
                     LQIDSRNWRALAALGRLRENQGDLAQALQNYQAAYASNRFLPELQAKIASLQSRVSTQ
                     PGAPPLASPAAPSRSAASPAPFRSF"
     gene            5454..>6317
                     /locus_tag="VFB80_07625"
     CDS             5454..>6317
                     /locus_tag="VFB80_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZN33672.1"
                     /translation="MPASLAVSDYSSALEFLLGRINYERTANIPYRSTEFKLDRMRRL
                     MALLGDPQLGLKAIHIAGTKGKGSTAAMIASVLSAAGLRTGLYTSPHLARTEERLAID
                     GHDCPRETFVALAAELQQAVEWLESEAAGDPALAGATYFEITTAMAFLHFARANVAAA
                     VLEVGLGGRLDSTNVCRPIVCLITSISFDHMRQLGHTLAEIAGEKAGIIKPGVPVVSG
                     VLLDEPRLVITRRAAELQASLWQRGVDYDFSSRQRGSLRTGGESLDYFELAAQPRLRV
                     DGVQIQMLGEHQ"
BASE COUNT         1063 a         2116 c         2120 g         1018 t
ORIGIN      
        1 agcgcgggat gctggacgag acgctcgtca tctggggcgg cgaattcggc cgcacgccga
       61 tggcccagga cggcaagggc gagcagctgg gccgcgatca ccacatgaag ggcttttcga
      121 tctggctggc cggcggcggc atcaagccgg gattcacgtt tggcaacact gacgacctgg
      181 gctatcacgc cgccgagaac atcgtccagg tccacgacct gcacgccacc gcgctctacc
      241 tgctcggcct cgaccacgag cggttcacct accgctacca gggccgcgac ttccggctga
      301 ccgacgtcag cgggcgggtc gtgagcgaga ttctggcgtg accggtcggt cggttgatcg
      361 gtagtcggtt ggttggtggt gctcactgac tcaccgaccg accgacttac cgaccgaccg
      421 atctaccgaa ccacgcggat cgccgcagcg aactcttgat tacgagagag cacggcgatc
      481 cgccgatcgt gggcggccat ccactcgcgg agggcccgcc actcgccctc ttcccaggcg
      541 tcgtacacgc cggagttgcg ccagtcgcac aattcgtcga acacgatgat cgtgcccggg
      601 acgatgcggt cgttgagttg cgtgagcgtg tcgatcgtcg agcagtagag atcggagtcg
      661 ttgtggagca gggccaccgg cccggcgtgt gcagcgcgcc agccgggcag cgtggcgtcg
      721 aagaaacccg gaacgagtag ggcgttcggc aggaccgccg gcagccgctc gagcgcgaag
      781 tggccgcggg cgtagctttc gccgctcttg gtgcggaccc acgcttcggg caggccctcg
      841 aacgagtcga agccaaaaac ccgcggctgg cggcgttgct cggcccagcg ggccagccaa
      901 ttcagcgagc cgccgcgata gacgccgaat tccaggatgt acccgtccgc cggagcgagc
      961 tccaaggcgt gatgcaagtg atcgagtcga ttcaggtagc aaggcgcggc gcgaaactgg
     1021 tcgatgtgca actcgtcggc cgctcgcgat tgcgaagtat ccgccgtgac tccgggagct
     1081 atgcccagtt gttcccagca atcgaagtgc ggccggccat cgcgcatggc gcggcggcgc
     1141 ggccacctgc cgccggtgac gcgccgcagc agctgagtaa gtccgctcat cgagagattt
     1201 cctggtgcga tgacctaacc ggcaaggcgt ccggagaaga agccgcgaat ccgccgggcg
     1261 atcgatcgag gcggcgcgag caccagctcg tcgccgtccc actgcagttg ccggatatca
     1321 ccgcaaaatc cctggcggat cggcagccgt ggatgctggg ggaaaaacgg ggcgagcaaa
     1381 tcgatgtcgc cctcgtcgta gtgcagctcg cgatacggaa tcgccgcggg aatcttgggc
     1441 gtgacgacgc tgatgttgta gccgtactgc ttgacgcggg cctggcggca gtcgaagcct
     1501 gcgaggacga ggttgtagag cacgaggccc gcattccaga gcgtgacgtg gccgccgacg
     1561 attgcgtgct tgagcggcgg cacggtcacg gccagcacgc cgccctcgcg caggtccgca
     1621 aacaccttgc ggaggaagaa attgacgttg agctggtgct ccagcacgtg cgacagccaa
     1681 aggcaatcga aaggccgctg gaattcgtgc cgcagatagt cggcgacgat cacgccctcg
     1741 gccaggccga cgatgtcgat gccgctcgcc gttttgccgg acgatcgaaa gcgctccaaa
     1801 tggcgaccag gcccgcagcc gacgtcgagg accgtggtga aatcgtactc gccgagcagt
     1861 ttctcgagcg cttcggccgc catgagcggc cgggacgcgg gcgtgtgagc tctgggcatg
     1921 ccatcctccg ggcggtctca aaacacgggc cgcgcgggca aaaccttggg cggcaagtcc
     1981 cgcatccaac ggacaacgcc gtcgctcgcc cgccgccagg cgttggccgc caggccggcc
     2041 cagccactgg ctcgccgcgg ctcagcgcgg gcagcgccgg ccaacgcctc ttcgacaagc
     2101 ggcggcaact gccgcgggat ttcggcaact ggccactggt gcgacagaat ccggctgacg
     2161 acttcggccg ccggctcgcg aaacagatcg cagcggccgt ccgcgagcgt tttggtcgcg
     2221 gcccgcgtgg ccgcaggatc gtcatccagg cagtacaccg cgcccggatg gcgatagatg
     2281 gcctttccat agcacagtac cggcagcccg ttgccgagcg cctcgacgcc acacgacgag
     2341 ttgacacagg ccagggcccg cgcgccgctc attgcggccc gcagcgtcgg gctctcgtcc
     2401 cacgtgccgc cgagctggcg gacgcgctcg atgaactcct ccggcggccg ctcgcgcgga
     2461 tggtgccgca cgcggacgct cagcgccgag ctttggcaga tgaactccat gaagcgctgc
     2521 atgttcgcga accagggcga gagccgggtg atctgcgtat cgcggtcgag ctgcaatacg
     2581 accagcagat cgccttgttc gcgcaccggc agtggcgtca gcggcctgct cgtcagcggc
     2641 tcggccgccc agctcgcgtt ggcgttgatc cccgcccggt cgatttggat gtggcctcgc
     2701 tgcggccacc agcccagctc ggtgaagatc agcggcgtgc cgcggcggcg cagcgttgcc
     2761 atcgcccgat cgtatttcgg gttcaggccg ttccagagaa tcgccaggtc gcagcggaac
     2821 aggtatcgcc agtggggcac acggcccgac tgcggccccc gccagcgaaa gacgtcgtga
     2881 cccaagctac ggcacgtctg cgcgatcaag tccagcggct cgcccggctg cgggtcgcgg
     2941 cgggcttcca ccagaatcgt gctcatgcca accctccttg gccgcctcgc ggcgtgctag
     3001 cacgtgcggc actgctccga gtgccgaggg gaatcctagg ccatgccgcg gggtgggcgg
     3061 aagagcaatt caccggtcgc gggctacttc gcgcggagct cgaacgtcgg ccggtttgcc
     3121 tccgggctga tcgtcgcggc cagacccgat tcggccggct tgctgtagcg ctccggggcg
     3181 atccagactt tttgctcggc gtactcgctg ccgtcggtcg gccggacgta gagcacctgg
     3241 agggccgtca ccgcgacctg atattcaccc ggcctgacgc cgtcgctcgg ccggttcgtg
     3301 gtgagcgtga actggccctc ggcattcgtc ttgcccgtcg cggggcggcc gccggatttg
     3361 ggcaggaaca gcacctgcgc gtcggcgagc ggctgatcct ccagcagcac tcggccttcg
     3421 accggcacga ggccgggacc gcagccggca aacaacacgg acgcgatgag ccaaatagcg
     3481 cgatgcatgc tcgacggcaa acggagtgtg cctgctgcct tacgggaccg cggcgggctg
     3541 gccgtcggcc acgccgccca ggtattgata cgtccaatgg tcgatcgact ggttgagaaa
     3601 ccgcacgccg ccgtcgccga agcagaactg tgcgccgccg gcgtgcagcg acttgaagcc
     3661 cagcgagacg gtgtcgttgc agtccgtccg gcagccatcg acggtttgcc actgggcccg
     3721 cggaccgcac gagtcgtagt tgatcggcac gatcgtcgtc atcacgccgc tgccgttgtt
     3781 cgaatcggcc cagccggcat tggcgaccga cgagcagcgc ggccgcatct cgccgatgaa
     3841 gatcgtcgcg gagaggccgt cctgaacgtc ggaaatccgc gtcgccacgc gccgcggaaa
     3901 gctggcgccg ggcgtcgaat tgtggcggcc aaacggtccg ctcgccgcca ggtgcgggac
     3961 cttggcaatc gcgaacgggt tgtacgggtg atagcaagtg caattgcccc ggacgttgcc
     4021 gctcgcgctc aaggcgttcg gccccgccga gccggcatag ctgcacagtg cgttgcggtt
     4081 cgtgggatgc aggccttgat agtcgctgct gggacagacc agagccttga tccgaatctt
     4141 gcggatcaca ttgttgaagt gcaccggcgc ggtctgctct tcgatgatga ccttcgagaa
     4201 gtcgatcagg ctcagcgtgt tctgctgctc gtagtaaggc agcacgaggt ggatcatcga
     4261 gcccttgctt tccgtccgcg gcgcgggagc aggcggcgga ttgctgctgt cccaggcggc
     4321 ccacgagccg aacgggaaga tctggtaggt gtcgtgatag ccgtgcaggg ccagcgcgag
     4381 ttgcttcaga tggttggcgc actgcatgcg gcgggcggcc tcgcgagccg cctgcaccgc
     4441 cggcagcagc agcgccacga gcaggccgat gatcgccacg accaccagca gctcgaccag
     4501 cgtgaacgcc gcccgattgc ccatatcgca gtccccaaga ccgttgcctg tgcgatccac
     4561 aggccattca tcttaaacgc tagcaccacg cagtgtcatg acgttcagac gatcttgtcg
     4621 ccggcgcggt tgctttggta gtctcccacc ccgtgaaatc tccatcttct cttgccgccg
     4681 tcgcgtttgc gctgctggcc gcatcggccg gctgccggat ggtctcaacg ggccgcaacg
     4741 tcgatggtgt gcggtacttc cagcaaggca actatccggt cgcgattcag cgattccagt
     4801 ccgcgatgac ggtcgactcg cggaacccgg attcgcacta taacctcgcg gccgtgaagc
     4861 accgcgaggg gctgatcaat cgcgaccaga attcgctgac ccaggccgag tcgctctatc
     4921 agcaggccct ggcctacgac cccaatcacg tcgatgccta ccgcggattg gcggtgctcc
     4981 tgaccgagac gggccgcacc agcgacgcct tcgccctgct caaccgctgg gccacgacga
     5041 accccggcag cgccgacgcc cgcgtggaga tcgcccggct gcacgaggaa tacaaccagc
     5101 cgcagcaggc ggaagcgtcg ctgcacagcg cgctgcaaat cgactcgcgg aactggcggg
     5161 cgctggccgc gctgggccgc ctgcgcgaga accagggcga tctggcccag gcgctgcaaa
     5221 actatcaggc ggcgtacgcc agcaaccgct tcctgccgga gctccaggcc aagatcgcca
     5281 gcctgcaaag ccgcgtctcg acgcagcccg gcgctccgcc gctcgccagc cctgcggcgc
     5341 ccagccgcag cgccgccagc cccgcgccgt tccggtcgtt ctaggctcgc tgccgggccg
     5401 ctggctcgcg cgatacactg gggcatgctt cgcgtccccc gccgcgccca gccatgccag
     5461 cttcgctcgc cgtcagcgac tattcctcgg ccctggagtt cctgctgggg cggatcaatt
     5521 atgagcggac ggccaacatt ccttaccgct cgaccgaatt caagctcgac cggatgcggc
     5581 ggctgatggc cttgctgggc gatccgcagc tcgggctgaa ggcgatccac atcgccggca
     5641 ccaagggcaa gggctcgacg gcggcgatga tcgccagcgt gctctcggcc gctgggctgc
     5701 ggacggggct ctacacttcg cctcatctgg cccgcacgga agaacgcctg gcgatcgacg
     5761 ggcacgattg cccgcgcgag acatttgtcg cactggcggc cgagttgcag caggcggtcg
     5821 agtggctgga atccgaagca gccggcgacc cggcgctggc cggcgcgacg tactttgaaa
     5881 tcacgactgc gatggccttt ctgcatttcg cccgggcaaa cgtggccgcc gcggtgctgg
     5941 aagtcggact gggcggtcgg ctcgattcga caaacgtctg ccggccgata gtctgcctga
     6001 tcaccagcat cagcttcgac cacatgcggc agctgggcca tacgctcgcc gagattgccg
     6061 gcgaaaaggc agggatcatc aagcccggcg taccggtcgt ttcgggcgtg cttttggacg
     6121 aaccgcggct ggtgataacg cgccgcgcgg cggagctgca ggcttcgctc tggcagcgcg
     6181 gcgtggacta tgacttctct tcccggcagc gcgggagcct ccgcactggc ggcgagtcgc
     6241 tggactattt cgaactggct gctcagccac gacttcgcgt agatggcgtc cagattcaga
     6301 tgctgggcga gcatcag
//
LOCUS       DASRJB010000284         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2619844, whole genome shotgun sequence.
ACCESSION   DASRJB010000284 DASRJB010000000
VERSION     DASRJB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619844"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFB80_07630"
     CDS             complement(<1..524)
                     /locus_tag="VFB80_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33673.1"
                     /translation="MPLVARHLALVAALALCARGASLAAADRYEAWLADGTKLSGPFI
                     PAWPVPGSPYRFESQDLLTAKNPLRLMRDRTLSTVLRPPFVVLANGDILSGAPVALEP
                     TQGRLAHVQRVKVQLEPPLLPVVGTTLAVRADRIARVVLTSEAARANPPPGTVVLADG
                     RRLTARSLRWREGGL"
     gene            complement(796..2034)
                     /locus_tag="VFB80_07635"
     CDS             complement(796..2034)
                     /locus_tag="VFB80_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33674.1"
                     /translation="MARPIGEAADAPLRWKAKNVGVAMDGEALPAVPIHGAQPVRTAA
                     FSTSTTRSNWNITRIDPSVRPAQAISDPFRDPFGDRKSGQSAAGHTAQPAAGHTEPLL
                     LQPTQAEASVEELPPPKAIGGPAPRVATQPSPLISRTQEPAPMPPAAAPPIPGEAAPA
                     PGPALESAPSGAPCDKVYNERNCCDTLAGCKVFRDDLLSDTIRHISLDITPRFSPDLT
                     PEEDLAERTDKLRLAESITWRNRTGQVVATGKMVDLANNVVIIADDTGREVARLLLQD
                     LGEAELCYVTAYWRLPTECGLGGLRVAQRQWIPSAYLWTASALCHKPLYFEEVQLERY
                     GHTAGPIRGPFISGAHFFLNIAALPYKMAINPPHECQYALGYYRPGSCAPWMIPPIPL
                     SLRGAAAETGAVLGGVFLIP"
     gene            complement(2213..3697)
                     /gene="guaB"
                     /locus_tag="VFB80_07640"
     CDS             complement(2213..3697)
                     /gene="guaB"
                     /locus_tag="VFB80_07640"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZN33675.1"
                     /translation="MNDKLTGQGITFDDVLLAPRYSEVVPSEVGVSTLLTKRIPLNIP
                     MLSSPMDTVTEHEMAIALAKEGGLGIIHKNMTVELQTEEVNKVKRSANGIILDPETLR
                     PDEPVSRAQELMKKKNVSGFPITDASGKLVGILTRRDLRFLENGSQPIREVMTKDNLV
                     TAKGTVTLEEAEKILTAKKVEKLLLVDDTSCLTGMITIRDIDMMKRFPSACMDKQGRL
                     RVGAAIGVYDFERAASLIQTGVDVLVVDSAHGHSKNVIETVKQLKKRSEIDVVAGNVA
                     TREGCRDLIAAGADAVKVGIGPGSICTTRVISGVGVPQISAVHEATQAAKASGTPIVA
                     DGGIRYSGDMTKAIAAGAHCVMIGGLFAGLAESPGKTILYQGRTFKAYRGMGSLGAMV
                     KGSSERYRQAGSEGGTGKLVPEGVEGRVPFKGPLSDFVYQLVGGLRAGMGYCGTKTIE
                     ELRTDTRFIQVSAASVRESHPHDIAITQEAPNYSPEYHDGKGGD"
BASE COUNT          577 a         1286 c         1245 g          686 t
ORIGIN      
        1 aggcccccct cacgccagcg aagcgagcgg gcggtgaggc ggcggccatc ggcgagcaca
       61 accgttcccg gcggcgggtt ggcgcgggcc gcttcgctgg tcaggacgac ccgggcaatg
      121 cggtcggccc gtacggcgag cgtcgtgccg acgacgggca gcaacggcgg ctccagctga
      181 accttgacgc gctgcacgtg cgcgaggcgg ccctgagtcg gctccaaggc gaccggcgca
      241 cccgacagga tatcgccgtt ggccaggacc acgaacggcg gtcgcagcac tgtgctcagc
      301 gtgcggtcgc gcatcagccg cagcgggttc ttcgcggtga gcaggtcctg gctttcgaac
      361 cggtagggcg atcctggcac cggccaggcc ggaatgaacg gcccgctcaa cttcgtgccg
      421 tccgccagcc aggcttcata cctgtcggct gctgccagcg atgcgccgcg cgcgcacagg
      481 gccagcgcag cgacaagagc gagatggcgg gcgacgagcg gcatcgcggg cgggctttcg
      541 gcgggctctt aggggtatgc ctcggagagg aagcttcatc ttgcgccagc ggcaatttcg
      601 ggtcaaccaa gaaacaccgg gccggcccgc tcgtttcgcg aagcgcccca agaaatgcaa
      661 tacggcggaa ccttgcggct ccgccgtttg ctgcgccaat cgctcccagt ccagttagtc
      721 ggtggtcggt tggtcggtgt tcatgtcacc gacttaccga ccaaccgacc accgactaac
      781 cgaccgaccg accacctacg gaatcaagaa cactcctccc agcaccgcac cggtctcggc
      841 ggcggcgccg cggagcgaga gcggaatcgg cgggatcatc cagggggcac agctgccggg
      901 ccggtaatag cccagggcgt actggcactc gtgcggcggg ttgatcgcca tcttgtacgg
      961 caaggcggcg atattcagga agaagtgcgc gccgctgatg aacggcccgc gaatcgggcc
     1021 ggccgtgtgg ccgtaacgct ccaactggac ttcttcgaag tacagcggct tgtggcacag
     1081 ggccgacgcc gtccacaggt acgcactggg aatccactgg cgctgcgcca cccgcaggcc
     1141 gcccaggccg cactcggtcg gcagcctcca gtaggcggtg acgtagcaca attcggcttc
     1201 gcccaggtcc tgcagcagca ggcgggcgac ttcgcggccg gtgtcgtcgg cgatgatgac
     1261 gacgttattg gcgaggtcga ccatcttgcc cgttgccacg acctgtcccg tgcgattgcg
     1321 ccaagtgatg ctctcggcca gccggagctt gtcggtccgc tcggccaggt cttcctcggg
     1381 cgtgaggtcg gggctgaacc gcggcgtgat gtcgagcgag atatggcgga tcgtgtcgga
     1441 gagcaggtcg tcgcggaaga ccttgcagcc ggccagcgtg tcgcagcaat tgcgttcgtt
     1501 gtagaccttg tcgcacgggg ctccgctcgg cgcggattcc aatgccggac cgggcgccgg
     1561 cgcggcctcg ccgggaatcg gcggggcggc ggcgggcggc atcggggccg gctcctgcgt
     1621 ccgcgagatg agcgggctgg gctgcgtcgc gactcgcggc gcgggcccgc caatggcctt
     1681 gggcggcggc aattcctcga ccgatgcctc ggcctgtgtc ggctgcagga gcagcggctc
     1741 ggtatgaccg gcggcgggct gcgcggtatg accggcggcg gactgtccgg acttccggtc
     1801 gccgaacgga tcccggaacg gatcgctgat ggcttgcgcc ggccgcacgc tgggatcgat
     1861 ccgcgtaatg ttccagttcg agcgcgtcgt cgatgtcgag aaggcggccg tccgaaccgg
     1921 ctgtgcgccg tgaatcggca ccgccggcag cgcctcgccg tccatggcca cgccaacatt
     1981 tttggccttc caacgcagcg gcgcatcggc cgcctcgcca atgggccgcg ccatgacgtc
     2041 ggccacaaag ggccggggct cttcgccgtg ggcgatggct gccaggagga ggaggtaagc
     2101 ggcggtcaga atgcggaatg cggaatgcgg aatgcggaat gcggcaggac cgaattccac
     2161 tggctcctgg cccctggctc ctggcccctg gccccgggcc cctggctctt gctcagtcgc
     2221 cgcccttccc gtcgtgatac tcggggctgt aattcggtgc ttcctgcgtg atcgcaatgt
     2281 catgggggtg gctctcccgc acgctggcgg ccgagacttg aatgaaccgc gtatccgtac
     2341 gcagctcttc gatcgttttg gttccgcagt aacccatgcc ggcccgcaag cccccgacca
     2401 gctgatagac gaagtcggag agcggccctt tgaagggcac gcggccttcg actccctccg
     2461 gcaccagctt tccggttccc ccctcgctgc cggcctgcct gtacctttcg ctcgatccct
     2521 tcaccatcgc gcccaggctg cccattccgc gatacgcctt gaacgtgcgg ccctggtaca
     2581 aaatcgtttt gcccggactt tcggccaggc ccgcaaacaa gccgccgatc atcacgcagt
     2641 gcgccccggc ggcgatggct ttcgtcatgt cgcccgaata gcgaatgccg ccgtcggcga
     2701 cgatcggcgt accgctcgct ttcgcggcct gcgtcgcttc gtgcacggcc gagatctgcg
     2761 gcacgcccac gccgctgatc acgcgggtcg tgcagatcga tcccggcccg atcccgacct
     2821 tcaccgcgtc ggcccccgcg gcgatcagat cgcggcagcc ctcgcgggtc gccacgttcc
     2881 ctgccacgac gtcgatctcg gaccgcttct tcagctgctt gaccgtctcg atcacgttct
     2941 tgctgtgccc gtgggccgaa tcgaccacca gcacgtccac cccggtctga atcaggctcg
     3001 cggcccgctc gaagtcgtaa actccgatcg ccgccccgac ccgcagccgc ccttgcttgt
     3061 ccatgcaagc actggggaac cgcttcatca tgtcgatgtc gcggatggtg atcatgccgg
     3121 tcaggcaact tgtatcgtca accagcagca gtttctcgac ctttttagcc gtcaaaatct
     3181 tctcagcttc ctcaagggtc acagtcccct tagctgtcac caggttgtcc ttggtcatca
     3241 cctcgcggat cggctggctg ccgttctcca gaaaccgcag atcgcgccgt gtgaggatgc
     3301 cgaccagctt gccgctagca tcggtgatcg gaaacccgct gacgttcttc tttttcatca
     3361 gctcctgggc ccggctgacc ggctcgtcgg gccgcagcgt ctcggggtcg aggatgatgc
     3421 cgttagccga ccgcttgacc ttgttcacct cttcggtctg gagctcgacg gtcatgttct
     3481 tgtggatgat ccccaggccc ccttccttgg ccagggcgat cgccatctcg tgctcggtca
     3541 ccgtgtccat cggcgagctg agcatgggga tgttgagcgg gatgcgcttt gtcagcagcg
     3601 tgctcacgcc gacttcgctg ggcacgacct cgctataccg cggtgcgagc agcacatcgt
     3661 caaacgtgat tccctggccg gtgagcttgt cgttcatgga gtatgctcct tatcgtgggt
     3721 ccggcgtctc acgggtgcca cgtcccacgc ttgcgtgggc gtgctgcctc gacgaggctc
     3781 ctgtcgagcc gtaa
//
LOCUS       DASRJB010000285         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_364584, whole genome shotgun sequence.
ACCESSION   DASRJB010000285 DASRJB010000000
VERSION     DASRJB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364584"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(579..2396)
                     /locus_tag="VFB80_07645"
     CDS             complement(579..2396)
                     /locus_tag="VFB80_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZN33676.1"
                     /translation="MNQLRAELGWITDRCRRRFRSAQALSLAFKALNLALLAPLSAAI
                     LHFCLSRWGRASVGNFELIAFFVSPVGIAALLGVGAVLLATLYLELSGLIRVLADGRL
                     HWWQAFKSSTGLLHRLIELGLRQLALYLALALPFLAGIGLVYWLLWHGKDLNGLIILK
                     PPVFWLGAGLAGAIAVAYLLLALRCFLQRIYAVPILTFESKTSPREALESSIERSRGR
                     LFRTLSVIAAWAAISSIVTIALLGLLDWALLRLIGRGGSSLSMAAVVTGTALAIHTVA
                     AMLLSVVANVTFAATLLSLYRQVAPDQTLGDDLEQPQPSPKSLGWMVGGVLLVLAGAS
                     VIAGYLAIRDLKLNESLEITAHRAGAAGAPENTVAALKQAIVDRADWAEIDVQLTADK
                     ALVVMHDIDLARVGGGNRRVDQATLAEIQALDVGASFGPNFAGERIPTLGDILAAAKG
                     KIRLNVELKPHGKSDADELTRRTIEELHSADMVPNCRLCSQSYESLQLARQIEPRLPV
                     GYIVATSVGDIAKLDVDFLMVKGSLVTRQLVDRARLRNIAVHAWTVNDPAEVGPLLDA
                     GVANLITDKPAPIRAEFDEIRALDVAPRLILRTRHSLSN"
     gene            complement(2413..>3259)
                     /locus_tag="VFB80_07650"
     CDS             complement(2413..>3259)
                     /locus_tag="VFB80_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HZN33677.1"
                     /translation="DGLRCDMAMLMCNDIFARTWGGTPPAEEYWPPLIARVKAAYPEF
                     LFIAEAYWDMEWTLQQQGFDYAYDKRLYDRLREHHAKPVREHFLAGLDYQDKLARFLE
                     NHDEPRAAATFPPGVHEAAAVLTFLSPGLRFLHQGQLEGRTKRISPHLGRGPNEPTNA
                     AIQQFYQRLLTILKQPVLRDGAWQLLECTPGWDGNWTQDNLIAFAWQRDTGERLIVAV
                     NYSPNQSQGHVRLPLADLAGKPWRLHDQVSGANYDWNGDDLVGRGLYLDLTPWQAAVL
                     EPRLS"
BASE COUNT          589 a         1050 c         1069 g          551 t
ORIGIN      
        1 gccgggccac cgctaccgaa aatggcgagg cggacactgg cgaggacgcc agtgccaccc
       61 acgccgaggt gccggccctc cgggcctcgc acgaatcgag tttccctctc gccatccccg
      121 gcctgacggc cggggctatt acctgccggc cctccgggcc tgccgcgggg cgcccgcgcg
      181 accaccgacc accggccacc ggccgccaca ctctctcacc cagactcgag tctgccaagc
      241 aacttgtaca catttagttc actacatgcc aacttgcgtc gatcgcgtgc ggcgcaagct
      301 ccttccccgc ctgcacttcc gcccccgccc cgcgccctaa gtttttttcg gggccgctag
      361 ccgaatggac tagtctggtc cgccgccccc cgccccgaag agcgagactt cttgccccac
      421 cctcaaagcc gcgaccactg gtcgcggctt tggggccgag gtcggctaaa ttgaggagat
      481 gcgaacggac ctttggggag agccgatgcc ggggtcgcgc cgctcgtttc cgctggcggc
      541 gctgtttcgc agcgctcata ttggcaacac acgcgtgact aatttgaaag cgaatggcgc
      601 gtgcgcagga ttaatcgcgg agcaacgtcc agcgcgcgaa tttcatcgaa ctcggctcgt
      661 atgggtgctg gcttgtcggt gatcagattg gcaacgccgg cgtcgagtag tggaccgact
      721 tctgccggat cgttgaccgt ccaggcatgc acggcgatgt tgcggagccg cgctcgatcg
      781 acgagttggc gcgtgacgag gctgcccttg accatgagga agtccacgtc cagtttggcg
      841 atgtcgccga ccgacgtagc gacgatgtaa ccgaccggca gccgcggctc gatttggcgg
      901 gccagttgca ggctctcgta ggactgggag cagagccggc agttggggac catgtcggcg
      961 ctatgcagtt cttcgatggt ccgccgagtg agctcgtcgg cgtcggattt gccgtgcggc
     1021 ttgagctcga cgttcaggcg aatcttgccc ttggcggcgg cgaggatgtc accgagcgtc
     1081 ggaatccgct cacccgcgaa gtttggccca aaggaggcgc caacgtcgag ggcctggatc
     1141 tccgccagcg ttgcctgatc gacgcgccgg ttgccgccac cgacgcgagc caggtcgatg
     1201 tcgtgcatga cgaccagcgc cttgtcggcg gtaagctgta cgtcgatctc ggcccagtcg
     1261 gctcgatcga cgatggcctg tttcaaggct gcgacggtgt tttcaggcgc accggccgcg
     1321 cccgcgcgat gggcggtgat ctccaggctc tcgttcaatt tcagatcgcg aatcgccaga
     1381 tagccggcga tcacactggc tccggccagc accagcagca cgccgccgac catccagccg
     1441 agcgacttgg gcgatggttg tggttgctcg agatcgtcgc ccaacgtctg atccggcgct
     1501 acctgtcggt atagcgacag cagcgttgcg gcaaatgtca cgttggccac cacactcaga
     1561 agcatggccg cgactgtgtg gatcgccagc gccgttccgg tcacgaccgc cgccatcgac
     1621 agcgatgagc cgccccgtcc gatcagccgc agcaatgccc agtcgagcag acccaataga
     1681 gcgattgtca caatgctgct gatcgccgcc caggcggcga tcacgctgag ggtgcggaac
     1741 aatcggccgc gcgatcgttc gatgctggac tccagggcct cgcgcggact cgtcttggat
     1801 tcaaaggtaa gaatcgggac ggcgtaaatc cgctgcagga agcagcgcag cgcgagcaag
     1861 aggtaggcca ccgcgatggc ccctgccagc cccgcaccca gccagaaaac cggcggcttg
     1921 aggatgatga gcccgttcaa gtccttgccg tgccacagca gccaatagac cagaccgatg
     1981 cccgccaaga acgggagcgc cagcgccaga tacagggcca gttgccgcag acccagctcg
     2041 atcagccggt gcagcaggcc ggtggagctt ttgaatgcct gccaccagtg cagccggccg
     2101 tcggccagca cgcggatcag cccggagagc tccaggtaga gtgtggccag cagcactgcg
     2161 ccgacgccca ggagcgcggc aatgccgacg ggcgaaacga aaaacgcgat cagctcgaag
     2221 tttccgacag aagcccgtcc ccatcgcgac aaacaaaaat gcaatatggc ggccgatagt
     2281 ggagccaaca gcgccaggtt gagcgccttg aatgccagcg acagcgcctg cgcgctacga
     2341 aacctgcggc ggcagcggtc cgtgatccag cccagctcgg cccgcaattg gttcatggca
     2401 gaaatcgcga aatcaggaaa ggcggggctc gaggacggct gcttgccagg gcgtcaggtc
     2461 caggtagagg ccgcgaccga ccagatcgtc gccattccaa tcgtagttgg cgccgctcac
     2521 ctggtcgtgc agtcgccacg gcttgccggc caggtcggcc aagggcaatc ggacgtggcc
     2581 ctggctttgg tttggcgaat agttgacggc gacgatcagc cgctcgcccg tgtcgcgctg
     2641 ccaggcgaag gcaatcaggt tgtcttgtgt ccagttgcca tcccagccgg gcgtgcattc
     2701 gagcagttgc caggcgccgt cacggagcac gggctgcttc agaatcgtta gcagtcgctg
     2761 atagaactgc tggatggctg cgttggtggg ttcgttgggt ccgcgaccga gatgagggga
     2821 gatgcgcttg gtgcggccct ccaactgtcc ctggtgaagg aaacgcaaac cgggcgagag
     2881 aaacgtgagc acggccgcgg cttcgtgaac gccgggcggg aacgtcgcgg cggctcgcgg
     2941 ctcgtcgtgg ttttccagga agcgggccag cttgtcctga tagtcgaggc cggcgagaaa
     3001 atgctcgcga acgggtttgg cgtggtgctc gcggaggcgg tcgtagagcc gcttgtcgta
     3061 ggcgtagtcg aagccctgct gctggagcgt ccactccatg tcccagtagg cctcggcgat
     3121 gaagaggaac tccgggtacg ctgccttcac ccgcgcgatc agcggcggcc agtactcctc
     3181 ggccggcggc gtcccgcccc aggtgcgggc gaagatgtcg ttgcacatca gcatcgccat
     3241 gtcgcagcgc agcccgtcg
//
LOCUS       DASRJB010000286         7089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1775033, whole genome shotgun sequence.
ACCESSION   DASRJB010000286 DASRJB010000000
VERSION     DASRJB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775033"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFB80_07655"
     CDS             complement(<1..963)
                     /locus_tag="VFB80_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN33678.1"
                     /translation="MKILLYEHVTGGGGWQDDPFRPPSGPLLTEGRAMVQAIAADLAA
                     IEGVEVCTSRDSRLPELHPAGCSVMALDSDSATREWLQQSCTAADWTLLIAPETGGSL
                     LARCQLVEAAGGRLLSPSSAVVEIAGDKQTTAELLESKGIPVPRGCVINSAEELAAQT
                     ARLPAVIKPRVGCGSVGVRVLKSNPDLASLGISKPQRLEEYVPGLAASVSVLAGPKGL
                     VSLPACGQRLSQDGLYTYLGGRLPLSEALSARARRLAEAAVSALPPATGYLGVDVILG
                     AAGNGDRVIEINPRLTTSYVGLRAFAKCNLAAAMLAVAEGREPDL"
     gene            1327..2220
                     /locus_tag="VFB80_07660"
     CDS             1327..2220
                     /locus_tag="VFB80_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HZN33679.1"
                     /translation="MQGNHWGRRTFLQALAASGASCLAGGLSAQDGLKEPVYRVSKAN
                     DVPAVPMAERHPLDPALDMARDAVTLIQRDIVDYTATIVKRERIKGVLGEYEFMQAKI
                     RNRKVVDGKLDKPLSVYLKFVKPADIAGREVVWVEGQNNNKLRAHEGGLKGKLLPTVW
                     LDPDSALAMRGQLHPIYDVGIENLVTKLIERGEREKKYGECEVTFTPGAKINGRLCTV
                     LRVIHPTQRPHFEFHKAEIFIDDQMKIPLRYAAFYWPVKTGEELPVLEEYTYLDVKLG
                     LVGSPLLQDADFNSENPSYNF"
     gene            2351..3190
                     /locus_tag="VFB80_07665"
     CDS             2351..3190
                     /locus_tag="VFB80_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33680.1"
                     /translation="MTRLGRLGLRVLRGAAFVYVALLLVLLLLENTLLYPAPRFPDPR
                     GDWEATYLPHEDVEFASADGTKLHGWLVEHPAPRGVLLYLHGNGDCIGFLGPYLKDLR
                     DNHQLTVFAFDYRGYGKSAGSPVEAGILADGDAAQKWLAQRTGRPPADIILMGRSLGG
                     CVAVDLAARNGARAIILQNTTTSMPDAAARIYWFLPVRLLMKNRYDSLTKISRYKGPL
                     LQSHGTADELIPYELGQRLFAAAPCQPKQFFSISGAGHNDLEPAEYEAALAAFLAALP
                     PSK"
     gene            3195..3689
                     /locus_tag="VFB80_07670"
     CDS             3195..3689
                     /locus_tag="VFB80_07670"
                     /inference="COORDINATES: protein motif:HMM:NF007807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZN33681.1"
                     /translation="MNIRRVAPTDGEVLLDIWLRSVRATHSFVSEADIQSIIPLVRQY
                     LTSSGPDIWVLSSDAGAAMGFMGMSGNKMESLFLAPEFHRQGGGRRLVRHAQQLYGEL
                     TADVNEQNEAARRFYESCGFLVEGRSPVDEQGRPYPLLHMRLALPPQESLTRSAAAPD
                     TSTD"
     gene            complement(3649..4251)
                     /locus_tag="VFB80_07675"
     CDS             complement(3649..4251)
                     /locus_tag="VFB80_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HZN33682.1"
                     /translation="MLTVSAITDLHRECIRRWHEGPIDNPYADFLAVVCEQLSFNFRL
                     WHEEDKARSPSASDAEIAAVKRAIDKLNQQRNDWIEKLDDWITAELERRGIAAATSAP
                     QNTETLGSTIDRLSILALRIYHLGEQRDRADASPDHRQAVERKLAVALAQHDDLAKSA
                     QQLADDLFAGRKRHKTYRQLKMYNDATLNPYLYQARQQTA"
     gene            4343..5419
                     /locus_tag="VFB80_07680"
     CDS             4343..5419
                     /locus_tag="VFB80_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33683.1"
                     /translation="MDHLERLASQWHERAGELAAWTMARLVNRTDVWGRYVRRKGDDT
                     THVITAPFRDERGKVFLDAESLVKHFRRKQPSGQLGLHSAGSDLTSRWLAIDIDRHDY
                     DDDLTVSPEGNFAAASAWRQLLADRGLDPLLLDSNGIGGFHLLVVFAEPMSTDSVHQF
                     GKRLVADYERRGLERKPEVFPDSPTWDRYGDWLRLPGRHHSRPHYTRVWNDEPYAEPL
                     WLEGHDAIDRILATRLAPVALLESLGISRQPRTVCLDFDGVLHSYRSGWRGAEVIPDP
                     PIHGTREAIARLREHYRVVVYSARCRTPEGRRAVELWLARNDIQVDDICDYKPPALVY
                     VDDRAIRFRGDWQATVDEIRQFRK"
     gene            5629..5979
                     /locus_tag="VFB80_07685"
     CDS             5629..5979
                     /locus_tag="VFB80_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33684.1"
                     /translation="MNAPATDFHSTVGEIASLYDLAESDVLDEVIRAGCRSFWWEGNH
                     YDAEGIRRLVKDWWRWDVTPEVELSLSPAELERLEGQLSAYEEAGQKPLRKFVASQTQ
                     LPSLAAGRGKQRTG"
     gene            6002..6223
                     /locus_tag="VFB80_07690"
     CDS             6002..6223
                     /locus_tag="VFB80_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33685.1"
                     /translation="MNSQRLIEDIQELVSEAAVEIANLDSPENVVCRRLRAMQNRLQL
                     AAVGDGQRKFMPSEAHPDFLASGRSQQIG"
     gene            complement(6224..>7089)
                     /gene="eno"
                     /locus_tag="VFB80_07695"
     CDS             complement(6224..>7089)
                     /gene="eno"
                     /locus_tag="VFB80_07695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZN33686.1"
                     /translation="LLPAPMMNIINGGAHADNSIDLQQFMVMPLGFQRFSDALRCGVE
                     VFHHLKKVLHERKLNTAVGDEGGFAPDLKSNREALDLILTAIEKSGYRAGEQVRIALD
                     AAATEFYDEKKRTYNIDGKDVTGEQLIDIWAEWCANYPIISIEDGCAEDDWESWKKLT
                     QRLGSKIQLVGDDLFVTNTERLERGIREGIANSILIKVNQIGTLTETISAIHLAHRNG
                     YTSISSHRSGETEDATIADLAVALGTGQIKTGSASRTDRMAKYNQLLRIEEELDTAAL
                     YGGPLLLSRRK"
BASE COUNT         1208 a         2311 c         2293 g         1277 t
ORIGIN      
        1 caaatccggt tcgcggcctt cggcgacggc gagcatcgcg gcggccaggt tgcattttgc
       61 aaacgcccgc aggccgacat acgaagtggt cagtcgcggg ttaatttcga ttacgcggtc
      121 accattgcca gccgctccca agattacatc cacacccaag tagccggtcg ccggcggcaa
      181 ggcgctgacc gccgcttccg ccaggcggcg ggcgcgggcc gacagggcct ccgacagcgg
      241 cagccggccg ccgagatacg tatagagtcc gtcctggctc aaccgctgcc cgcaggccgg
      301 cagtgaaacc aggcccttgg gccctgccag cacgctgacg ctggccgcca ggccaggcac
      361 atactcttcg agccgctgtg gcttgctaat ccctagactc gccaaatcgg ggttcgattt
      421 cagcacgcgc acgcccaccg agccgcaccc gacgcgcggc ttgatgaccg ccggcagccg
      481 ggcggtttgt gcggccagtt cctccgcgct attgatgacg cagccgcgcg gaaccggtat
      541 tcccttgctc tccagcagct ccgcggtcgt ttgcttgtcg ccggcaatct ccacgacggc
      601 cgaagaaggc gaaagcagcc ggcctccagc ggcttccacc agttggcagc gagccaatag
      661 cgagccgccg gtttcgggcg cgatcagcag cgtccagtcc gcggcggtac aggattgctg
      721 aagccactcg cgcgtcgccg aatcgctgtc gagcgccata acgctacagc ccgccgggtg
      781 caactctggc aggcgggaat cgcgactggt gcagacctcg acgccttcga tcgcggccag
      841 atccgcagcg atggcctgca ccatcgcccg cccctccgtc agcagcgggc cgctcggcgg
      901 tcgaaatgga tcatcctgcc agccgccccc gccggtgaca tgttcgtaga gcaaaatctt
      961 caagggaacc ctattctggt ctcgcggcgg tcgggcgtcg gggcgtacaa tgccccgatt
     1021 ggcaaagctg ggtgattaca caggatttac gggcggatgg gacggcgaat tacgtctctg
     1081 gttttgtaat atccacttcg gctggagggc gatagtagca ataactgcca tggcgaggta
     1141 gctcttgagt cgcatcggcc acatcataaa tcccgtgccc gaaacgactt aagggtgaac
     1201 gccggctgcc gccgcagccg cggcccgctg tttgcagtcg cgccacacca gtttcaggga
     1261 cgacaccgac ggttgccgcg gcccagcgaa aggcggaacc gtcacactcg gaggggaagc
     1321 gagccgatgc aaggaaatca ttggggccgc cggacatttt tgcaagcgct ggctgcgagc
     1381 ggtgcgtcct gcctggcggg cgggctgtcc gctcaggacg gtctgaagga gcccgtctat
     1441 cgcgtttcga aggcgaacga cgtgcccgcc gtgccaatgg ccgagcggca tccgctcgac
     1501 ccggcgctcg acatggcccg cgacgcggtc acactgatcc agcgggacat cgtcgattac
     1561 acggcgacga tcgtcaagcg tgagcggatc aagggtgtgc tcggcgaata cgaatttatg
     1621 caggccaaga ttcgcaaccg caaggtcgtg gacggcaagc tcgacaagcc gctcagcgtg
     1681 tatctcaaat tcgtcaagcc ggccgacatc gccggccgcg aagttgtctg ggtcgagggc
     1741 cagaacaaca acaagttgcg ggcccacgaa ggcggcctga agggcaagct cttgcccacc
     1801 gtctggctcg atcccgacag cgccctggcg atgcgcggcc agctgcaccc gatctacgac
     1861 gtcggcatcg agaacctcgt caccaagctg atcgagcgcg gcgagcgtga gaaaaagtac
     1921 ggcgagtgcg aggtgacctt tacgcccggc gcgaagatca acggccgcct ctgcacggtg
     1981 ctccgcgtga tccatccgac gcagcgcccg cacttcgagt tccacaaagc tgagattttc
     2041 atcgacgatc agatgaagat tcctctgcgg tatgcggcct tttattggcc ggtaaagacc
     2101 ggagaagagt tgccggtgct cgaggaatac acctacctcg acgtcaagct cggcctcgtc
     2161 ggcagcccgc tgttgcagga cgccgatttc aattccgaga atccgagcta caatttctag
     2221 gcgtcgagcg caaactcgaa tcaccccggc ccggcgatgc agcatcgccg ggccggcttg
     2281 cttttcgggc acaaacgcgg aacttttgtg gtgacgcggg tataacaagc ttgtggccgc
     2341 caagccgatc atgacgcgac tgggccgatt gggcctgcgg gtcctgcgag gggcggcatt
     2401 cgtttacgtg gccttgctgc tcgtgctgct gctcctggaa aacacgctgc tttaccccgc
     2461 gccgcggttt cccgatccgc gcggcgattg ggaggcgacg tatctgccgc acgaggacgt
     2521 ggagttcgcc tcggccgacg gcacaaagct gcacggctgg ctagttgagc atcccgctcc
     2581 gcggggcgtg ctgctgtact tgcacggcaa cggcgactgc atcggattcc tggggccgta
     2641 tttgaaggat ctgcgcgaca accaccagct gaccgtgttt gccttcgact accgcggcta
     2701 cggcaaaagc gccggttcgc cggtcgaggc cgggatcctg gctgacggcg acgccgccca
     2761 aaagtggctg gcccagcgga ccggccgccc gcctgccgac attatcctga tggggcgctc
     2821 gctcggcggc tgcgtggccg tcgatctggc ggcacggaac ggggcccgcg cgatcatcct
     2881 gcaaaacacg accacctcga tgcccgacgc cgcggcccgc atctactggt tcttgccggt
     2941 gcggctgctc atgaagaacc gctacgactc gctgaccaag atcagccgct acaaggggcc
     3001 gctactgcag agccacggca cggccgatga attgattccc tacgaactgg gccagcggct
     3061 gtttgccgcg gcgccctgcc agcccaagca attcttctcc atctccggcg ccgggcacaa
     3121 cgacctggag cccgcagagt atgaagccgc cctggcggcc tttctggcgg cgctcccgcc
     3181 gagcaagtga ctcgatgaac atccggcggg tggctccgac agacggcgaa gtgctgctcg
     3241 atatctggct gcggtcggtg cgggcgacgc attcgttcgt atccgaggcc gacatccagt
     3301 cgatcatccc gctagtccgg caatacctca catccagcgg gcccgatatc tgggtgctct
     3361 cttccgatgc gggcgcggcg atgggattca tgggtatgtc ggggaacaag atggaatctc
     3421 tgttcctggc accggaattc caccgccagg gcggcggccg ccggctcgtc cgccacgctc
     3481 agcagctcta cggcgagctg accgccgacg tcaacgagca gaacgaagcc gcccgccgct
     3541 tctacgagtc gtgcggattc ctcgtcgagg gccgctctcc ggtggacgag caggggcggc
     3601 cctatccgct tttgcacatg cggctagcgc tgccgccgca agagtcgctc acgcggtctg
     3661 ctgccgcgcc tgatacaagt acggattgag cgtggcgtcg ttgtacattt tcaattggcg
     3721 gtaggtcttg tgccgcttgc ggccggcgaa caagtcgtcg gcgagctgct gagcggattt
     3781 cgccaggtcg tcgtgctgcg ccagcgcgac cgccagcttg cgttcgacgg cctgccggtg
     3841 gtccgggctg gcatccgcgc ggtcgcgctg ctcgccgagg tgataaatcc gcagggccag
     3901 gatcgaaagc cggtcgatcg tgctgcccag cgtctcggtg ttctgcggcg cggaggttgc
     3961 ggcggcaatg ccgcggcgct ccagctccgc cgtgatccag tcgtcgagct tctcgatcca
     4021 gtcgttgcgc tgctggttga gcttgtcgat ggcccgcttg acggctgcga tctcggcgtc
     4081 gctggccgac ggcgagcggg ccttgtcctc ctcgtgccag aggcggaagt tgaagctcag
     4141 ctgctcgcaa acgacggcca ggaaatccgc atacggattg tcgatgggcc cctcgtgcca
     4201 gcggcggatg cattcgcggt gcagatcggt gatggcgctg acggtcaaca tgaagtccct
     4261 ttggctaacg agaccgcggt atcgttgcag gtcacagcag tttctgccaa ggcgagttat
     4321 gatcgaatgc tgacgcattc ctatggacca ccttgagcga ttggcgagtc agtggcacga
     4381 gcgggcgggc gagctcgccg cctggacgat ggcccgcctc gtgaaccgca ccgacgtctg
     4441 gggccgctac gtccgccgca agggagacga cacgacccac gttatcaccg ccccgttccg
     4501 ggacgagcgg ggcaaggtct ttctcgacgc cgaatcgctg gtgaaacact tccggcgaaa
     4561 gcagccgtcc ggccagctgg gcctgcacag cgccggcagc gacctcacgt cgcggtggct
     4621 ggcgatcgat atcgaccggc acgactacga cgacgacctc acggtctcgc cggagggcaa
     4681 ctttgccgcc gccagcgcct ggcggcagct gctcgccgac cgcggcctcg atccgctgct
     4741 gctcgactcc aacggcatcg gcggctttca cctgctcgtc gtgttcgccg agccgatgag
     4801 caccgacagt gtccatcagt tcggcaagcg gctggtggcc gactacgaac gccgcgggct
     4861 ggagcggaag cccgaggtct ttcccgattc gcccacctgg gaccgctacg gcgactggct
     4921 gcggctgccc ggccgtcacc attcgcggcc gcactacacc cgcgtctgga acgacgagcc
     4981 atacgccgag ccgctttggc tggaagggca cgacgcgatc gaccggattc tcgccacgcg
     5041 gctggcgccc gtggcgctgc tcgagagcct cggcatctcg cggcagccgc ggacggtgtg
     5101 cctcgacttc gacggggttt tgcactcgta ccggtcgggc tggcgcgggg cggaggtcat
     5161 tcccgatccg ccgatccacg gcacgcgcga ggcgatcgct cgcttgcggg agcattatcg
     5221 ggtggtcgtc tattcggccc gctgccgcac gcccgaaggc cggcgggcgg tcgagctctg
     5281 gctggcgagg aacgacatcc aggtggacga catctgcgac tacaagccgc cggccctggt
     5341 ctatgtcgat gaccgggcga tccgtttccg cggcgattgg caggcgaccg tggacgagat
     5401 tcggcaattc cgaaagtaac gccgaaatcc agccggcagc cgggcccgcc ctttcccgtg
     5461 ggttgctttt tgtgctagcg ggtggcatgc tagcaggtgg attgccgctt cctgttgtag
     5521 gtcccgcctg ccgggcggga cctgggccgc gcggtgaggt gacgtcccct ggtcgctgcg
     5581 cttccctgga ggccccttcc ggcagaaggg cctacttgcg ggactgccat gaacgctcct
     5641 gcgactgact tccattccac cgtcggcgaa atcgccagtc tctacgacct ggcggagagc
     5701 gacgtgctgg acgaagtcat ccgcgcgggc tgtcggtcct tctggtggga aggcaaccat
     5761 tacgacgccg aaggcatccg ccgcctggtc aaggactggt ggcggtggga tgtgacgccc
     5821 gaggtcgagc tgtccctttc acctgccgag ctcgagcgat tggaggggca gctgtcagcg
     5881 tacgaagagg cggggcaaaa gccgcttcgg aaattcgtgg ccagccagac ccagctgccg
     5941 agcctggcag caggccgtgg gaaacaacgc acgggttgaa cacacaaacc agcgatatct
     6001 catgaattcg cagcgtttga tcgaagacat ccaggaactg gtcagcgagg cggccgtcga
     6061 gattgccaac ctggacagtc cggaaaacgt cgtctgccgg cggctgcggg ccatgcagaa
     6121 ccggctgcaa ctggccgccg tcggtgacgg gcagcgaaag ttcatgccca gcgaggccca
     6181 cccggatttc cttgcttccg ggcggtcgca gcaaatcggc tgatcacttc cgccgcgaca
     6241 atagcagcgg cccgccatac agcgcggccg tatcgagctc ttcctcgatc cgcaatagct
     6301 ggttgtactt cgccatccgg tcggtgcggc tggcactgcc ggtcttgatc tgcccggtgc
     6361 ccagggcgac ggccaggtcg gcgatcgtcg cgtcctcggt ctcgccgctg cggtggctgc
     6421 tgatgctcgt gtagccgttg cggtgggcca ggtggatcgc cgaaatcgtc tcggtgagcg
     6481 tgccgatctg gttcaccttg atcaggatgc tgttggcgat gccttccctg atcccacgct
     6541 ccagtcgttc cgtgttcgtg acgaacagat catctccaac gagctgaatc ttgctgccca
     6601 gccgctgcgt gagctttttc caactctccc aatcgtcttc ggcgcagccg tcctcgatgg
     6661 aaatgatcgg gtagttcgcg caccactcgg cccagatatc gatgagctgc tcgccggtga
     6721 catccttgcc gtcgatgttg taggtcctct ttttttcgtc gtagaactcg gtggccgccg
     6781 cgtcgagggc gatgcgaacc tgctcgcccg cccggtaacc cgacttctcg atggccgtca
     6841 ggatcaggtc cagggcctcg cggttgctct tcagatcggg cgcgaagccg ccctcgtcgc
     6901 cgacggccgt gttgagcttc cgctcgtgca gcaccttctt caggtgatgg aagacctcaa
     6961 cgccgcaccg cagggcgtcg ctgaaccgct gaaagcccag cggcatgacc atgaactgct
     7021 gcaagtcgat cgaattgtcg gcgtgcgcac caccgttgat gatgttcatc atcggcgccg
     7081 gcagcaggt
//
LOCUS       DASRJB010000287         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4030883, whole genome shotgun sequence.
ACCESSION   DASRJB010000287 DASRJB010000000
VERSION     DASRJB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4030883"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFB80_07700"
     CDS             complement(<1..932)
                     /locus_tag="VFB80_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33687.1"
                     /translation="MSMRSLFVAACLATLSSPLSAAEPAASELPWSFQPIARPNVPAV
                     QDVGWPRDDIDRFVLAKLEAAKLSPNPDADRAALIRRLAYDLTGLPPAEADLAENQTY
                     EALVDRYLNSPRFGERWGRHWLDVVRYADNTGRVWNAPLTYAWRYRDYVIDAFNKDKP
                     YDRFISEQLAGDLLPAKSVSEERENRIATGFLALGAHDLQTLNHEQFVMDGIDDQIDV
                     TTRAFLGLSVACARCHDHKYDPVTMRDYYALAGIFYSTTMLPGVAHQRESGGGGYVNP
                     TKLQILPTPAGRPVAGESGTHSMYDYQDRWRTGER"
     gene            complement(1011..1442)
                     /locus_tag="VFB80_07705"
     CDS             complement(1011..1442)
                     /locus_tag="VFB80_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN33688.1"
                     /translation="MSNNRQATVRLVDENAGGGKVREVFDDIKRTKQIDFVPALWRAL
                     ATNPDQLELVWKQLKALMHPEAAGRKSKLDPKTRETIALAVSATNGCSYCVNSHTAAL
                     KKLGVDTETLGEILAIAGLFNMTNALADGYQVEPDVLPPLE"
     gene            complement(1452..1655)
                     /locus_tag="VFB80_07710"
     CDS             complement(1452..1655)
                     /locus_tag="VFB80_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33689.1"
                     /translation="MTRKLVLAAVLCGCTCANGCYVADTVMQGQKAMKDVEALTPEVE
                     KRNKEVNELANPTAAPAEAPATP"
     gene            complement(1693..3147)
                     /gene="zwf"
                     /locus_tag="VFB80_07715"
     CDS             complement(1693..3147)
                     /gene="zwf"
                     /locus_tag="VFB80_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN33690.1"
                     /translation="MAHTVVIFGASGDLTSRKLIPALYSLHHKGRLPDQTRVVGVSRT
                     SFTSEAWREELAASTQQFAGTEFDSAAWQRFAGSLFYHAGDIDKLDDFAALAKFLNDL
                     EGHAGATRVYYLSTAPSLYAAAIANLGAAGLADESRGVRRVVIEKPFGRDLKSAQELN
                     RRAHQVFAEQQVYRIDHYLGKETVQNLLVLRFANSIFEPLWNRRYIDHVQITVAEEVD
                     VGRRAGYYDQAGVLRDMFQNHLLQLLMVVAMEAPARYTADFIRDEKVKVLRAIKPLAS
                     ADIARDTVRGQYEGYRQAEGVPQGSQTATFAAIKLQVDNWRWQGVPFYLRSGKVMSCR
                     TSQIVIQYRQPPIMMFDSGPRSHLDANKLVVQIQPAEGIQLHFQTKVPDAGMRLRTTD
                     LDFKFAREFTGAMPDSYQRLLLDAMTGDASLFARSDEVEVAWSIIDPILAAWQTPSAP
                     PLEIYPRGDWGPAASDEWMKAQGREWFDVCPVLH"
     gene            complement(3151..3942)
                     /locus_tag="VFB80_07720"
     CDS             complement(3151..3942)
                     /locus_tag="VFB80_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN33691.1"
                     /translation="MSNSAEFLAHLFGLQDQVAVVIGGTGVLGGALCEGLARAGARVV
                     VAGRSEERGHERVQAIEAAGGEATFLPVEADQRLSVQALLDSSITAFGQIDCLVNCAG
                     ANSATPYEQLSDDDWRRVIDSNLLATHLGCQIFAPFMASQASGGSILNIGSVTAHLPL
                     SRVFAYSASKAAVVNLTKNLAREFAPRNVRINVLCPGFFPAEQNRKILDQQRVENIMR
                     GTPMNRFGEPNELVGPALLLLSQRAGSYVTGEDLYVDGGFTSMRF"
     gene            4105..4776
                     /locus_tag="VFB80_07725"
     CDS             4105..4776
                     /locus_tag="VFB80_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33692.1"
                     /translation="MIRYIGIALVVAAAIQAPQTAAGQSEAAQGVVEYVQGRPGRTGN
                     LGTTLYAPSEREKPFFKRLAKGEVVTGGLAGDYDITKKDKTYVGWFGIIREIKEDKEK
                     GQTVLLLEHKYFDGLTDLHILALSFNGSGDFQAVVPGVGYKLQPLALVKVYGTVSGPA
                     GDSLPRVTAEFVRHWDWGTFTFLMAAGTQRGSEKWRKANRVDLDRIYEPYPDDRYYEM
                     RLGKR"
     gene            complement(4780..4950)
                     /locus_tag="VFB80_07730"
     CDS             complement(4780..4950)
                     /locus_tag="VFB80_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33693.1"
                     /translation="MDITNSGFAGDGDAVVAQALDSTQGFSLVLAGLKALLEHNIRLN
                     LVPDRFPKGSDH"
BASE COUNT          867 a         1599 c         1735 g          904 t
ORIGIN      
        1 cgttcgccgg tgcgccagcg gtcctggtag tcgtacatcg aatgcgtgcc gctttcgccc
       61 gcgactggcc gcccggctgg cgtcggcaga atctggagct tggtcgggtt cacgtacccg
      121 ccgccgccgc tttcgcgctg atgggcgact ccgggcagca tcgtcgtgct gtagaagatc
      181 ccggcgaggg cgtagtagtc gcgcatcgtg accgggtcgt acttgtgatc gtggcagcgg
      241 gcgcaggcca ccgacaggcc gaggaaggcc cgcgtcgtca cgtcgatctg gtcgtcgatg
      301 ccgtccatga caaactgctc gtggttgagc gtctgcagat cgtgagcgcc gagcgcgaga
      361 aagccggtcg cgatgcggtt ctcgcgctcc tccgacacgc tcttggccgg caacaggtcg
      421 cccgcgagct gctccgagat gaaccggtcg tacggcttgt ccttgttgaa cgcgtcgatg
      481 acgtagtcac gataccgcca ggcataggtc agcggggcgt tccagacgcg gcccgtgttg
      541 tcggcatagc ggaccacatc gagccagtgc cgcccccacc gttcgccgaa gcggggcgag
      601 ttgaggtagc gatcgacgag cgcctcgtac gtctgattct cagccaggtc ggcctccgcc
      661 ggcggcaacc ccgtcaggtc ataggccagg cggcggatca gcgccgcgcg gtcggcatcg
      721 ggattcggcg acagcttcgc ggcttcgagt ttcgccagca cgaaacggtc gatgtcatcg
      781 cgcggccagc caacgtcttg caccgcgggc acgttcggcc gcgcgatcgg ctggaaggac
      841 caaggcagct cgctggccgc tggctctgcg gcgctgagcg gcgagctcaa cgtggcaagg
      901 caagcggcga cgaagaggct tcgcatactc atgaaatcct cgatcgtcgc attctacggg
      961 caaacccgta gaggtcaacc gacgacgctg gcagcctcct caacgaccgc tcattcgagc
     1021 ggcggcaaaa cgtcgggctc gacctgatag ccgtcggcca gcgcgttggt catgttgaac
     1081 aagccggcga tcgccaggat ttcgcccagc gtttccgtat cgacgccgag cttcttgagt
     1141 gccgcggtgt gcgagttgac gcagtaggag cagccgttgg ttgccgagac ggccagggcg
     1201 attgtctcgc gggtcttggg atcgagcttg ctcttgcggc cggcggcttc cgggtgcatc
     1261 agcgccttga gctgcttcca gaccagctcg agctgatcgg gattcgtcgc caaggcccgc
     1321 cacagcgccg gcacgaagtc gatctgcttg gtccgcttga tgtcgtcaaa gacctcgcgg
     1381 acttttccgc cgccggcgtt ctcatcgacg agtcgcaccg tggcctggcg attgttggac
     1441 atgggcatcg ctcacggagt ggcaggggcc tcggcggggg cggccgtcgg gttggccaac
     1501 tcgttcactt ccttgttccg tttttcgact tccggtgtca gtgcttcgac gtctttcatc
     1561 gccttctgtc cttgcattac cgtgtcggcg acatagcaac cgttcgcgca ggtacagccg
     1621 caaagcaccg ccgcgaggac gagttttcga gtcatgaccg gagtttctcc gcggcgcggg
     1681 tgagactgga gctcagtgta acaccgggca gacgtcgaac cactcccgcc cctgggcctt
     1741 catccactcg tcgctggccg ccgggcccca gtcgccgcgc gggtagattt ccagcggcgg
     1801 ggcggagggc gtttgccagg cggcgaggat cgggtcgatg atgctccagg cgacttcgac
     1861 ctcgtcgctg cgggcaaaca ggctggcgtc gccggtcatc gcatccaata gcagccgctg
     1921 gtacgagtcg ggcatcgcgc cggtgaattc gcgggcgaac ttgaagtcga ggtcggtcgt
     1981 ccgcagccgc atgccggcgt cgggcacttt ggtctggaag tgcagctgga tgccctcggc
     2041 cggctgaatc tgcacgacga gcttgttggc gtccaggtgc gagcgggggc cgctgtcgaa
     2101 catcatgatt ggcggctggc ggtactgaat aacgatctgc gaggtgcggc agctcatgac
     2161 ctttcccgac cgcaagtaga agggcacgcc ttgccagcgc cagttatcga cctgcagctt
     2221 gatcgcggcg aaggtcgcgg tctggctgcc ctgcggcacg ccctcggcct ggcggtagcc
     2281 ttcgtattgc ccgcgaacgg tgtcgcgagc aatgtcggcg gacgccagcg gcttgatggc
     2341 ccgcaggacc ttcaccttct cgtcgcggat gaagtcggcc gtgtagcggg ccggggcctc
     2401 catggcgacg accatcaaga gctgcaagag gtggttctgg aacatgtcgc gcagcacgcc
     2461 cgcctggtcg tagtagccgg ccctgcggcc gacatcgacc tcctcggcga ccgtgatttg
     2521 cacgtggtcg atgtagcggc ggttccagag cggctcgaag atcgaattgg cgaaccgcag
     2581 cacgagcagg ttctgcacgg tctctttgcc gaggtagtgg tcgatgcgat agacctgctg
     2641 ttcggcgaat acctggtggg ccctgcggtt gagctcctgg gccgatttca ggtccctgcc
     2701 aaagggcttt tcgatgacca cccgccgcac gccgcgcgat tcgtcggcca gtcccgccgc
     2761 gccgaggttc gcaatggccg cggcatagag gctcggcgcc gtggagaggt agtagacgcg
     2821 cgtcgcgccc gcgtggccct cgagatcgtt caggaacttg gccagcgcgg cgaagtcgtc
     2881 cagcttgtcg atgtcgcccg catgatagaa gaggctgccc gcaaaacgct gccaggctgc
     2941 cgaatcgaac tccgttcctg caaattgctg cgtggatgcc gccagctctt cccgccaggc
     3001 ttcactcgtg aagctcgtcc gcgacacgcc gacgactcgc gtctggtccg gcagccggcc
     3061 cttgtgatgc agcgagtaga gcgccggaat cagcttgcgg ctggtgaggt cgcccgaggc
     3121 gccgaagatg acgacggtat gagccatagg ctagaaccgc atcgacgtga acccgccatc
     3181 cacgtataag tcttcgcccg tgacgtacga gccggcccgc tgggagagga gcagcagggc
     3241 gggcccgacc agctcgttcg gctcgccgaa gcgattcatc ggcgtgccgc gcatgatgtt
     3301 ctcgacgcgt tgctggtcga ggattttgcg gttctgctcg gccggaaaga agccggggca
     3361 aagtacattg atccgcacgt tccgcggggc gaactcgcgg gccagattct tggtgaggtt
     3421 gacaaccgcg gccttggacg ccgaataggc aaagacccgc gacagcggca ggtgggcggt
     3481 gacgctgccg atattgagga tcgagccgcc actggcctgc gacgccatga agggggcgaa
     3541 aatctggcag cccaggtgcg tcgccagcag gttggagtca atcacccgcc gccaatcgtc
     3601 gtcgctgagc tgctcgtagg gcgtggcgga gttcgcccca gcgcagttca cgaggcagtc
     3661 gatctggccg aaggcggtga tcgacgaatc gagcagggcc tgcacgctca atcgctggtc
     3721 ggcctcgact ggcagaaagg tcgcctcgcc gccggcggcc tcgatcgcct ggacccgctc
     3781 gtggccccgc tcctcgctgc ggccggcgac gacgacccgc gcgccggcgc gggccaggcc
     3841 ctcgcacagc gcccctccca ggactcccgt gccgccgatc accacggcga cctggtcctg
     3901 aagtccgaat aggtgagcga gaaactcggc gctgttcgac atgaaacgat ccacctcgcg
     3961 ttcgggcaac ggggcaacca caaaaggtaa cgaaggcacc gaagggcaca caagaaggcc
     4021 gacctgcggc ccgaacaatg gcaggtggca tcgtaccgcg ttacattctg gtggggcact
     4081 ccctttaaga gcgaggatgc gcacgtgatt cgctacatcg ggatcgcgct ggtcgtcgcg
     4141 gcggcgatac aagcgccgca gaccgcagcc ggacagtccg aggccgcaca gggcgtagtc
     4201 gagtatgtcc agggccggcc ggggcggacg ggcaacctgg ggacgaccct gtacgcgccg
     4261 agtgaaaggg agaagccgtt tttcaaacgg ctcgccaagg gcgaagtggt tacgggaggc
     4321 ctggccggcg actacgacat caccaagaag gacaagacat acgtgggctg gtttggcatc
     4381 atccgcgaga tcaaggaaga caaggagaag ggacagacgg tcctgctcct ggagcacaag
     4441 tattttgatg gtcttaccga cctgcacatt ctcgctctgt ccttcaacgg ctccggcgac
     4501 tttcaggccg tcgtgcccgg cgttgggtac aagctgcagc cgctggccct ggtcaaggtc
     4561 tatggcacgg tgtccgggcc ggcgggcgat tcgctgccgc gggtgaccgc cgaatttgtg
     4621 cggcactggg actgggggac gttcactttc ctgatggccg ccggtacgca gcgcggcagc
     4681 gagaaatggc gcaaagcaaa ccgcgtggac ctggaccgca tctacgagcc gtatccggac
     4741 gacaggtatt acgaaatgcg gttggggaag cggtagtgct cagtggtcgc tgcctttcgg
     4801 gaagcgatcg ggcaccaggt tcagacggat gttgtgctcg agcagcgcct tgaggccggc
     4861 gagcaccagg gagaagccct gcgtcgaatc gagcgcctgc gccacgaccg cgtcgccgtc
     4921 tcccgcgaag cctgagttcg tgatgtccac gaacgtgccg tcgtcgcggg aggtgtagcg
     4981 ccattcgacg gtagtcggct cgttccggcc cggccactcg atgcggaagg agctcggcta
     5041 cttcgcatcg gccgtgacgc ccttccaatc gtcagtgcgg aacggcgtca gcggcaggcc
     5101 ctcgc
//
LOCUS       DASRJB010000288         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3748288, whole genome shotgun sequence.
ACCESSION   DASRJB010000288 DASRJB010000000
VERSION     DASRJB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748288"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFB80_07735"
     CDS             <1..307
                     /locus_tag="VFB80_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33694.1"
                     /translation="PWYQIPPLDFVSENMVAREIRDERNVDVQLVPEEIVPQQKLLDR
                     AQALRDGAMAGYVTPLLNGGPTAPPSREPPPHIGVPGQGLPGGQPLPYEPLPNPGGP"
     gene            complement(319..828)
                     /locus_tag="VFB80_07740"
     CDS             complement(319..828)
                     /locus_tag="VFB80_07740"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HZN33695.1"
                     /translation="MSPPDLPAGPYVAEPHPTPARRGELIAEIEQLPARLRSLVGGLP
                     AGQLERRYKNWNVRQIVHHLADSHMNAFVRFRLTLTEERPTIKPYDETRWAELPDSKS
                     ADVTLSLQLLEALHARWVLLLRAMSDADFQRSYFHPEYQKEFRLAEVLGLYAHHGRHH
                     TGQIAWLSQ"
     gene            931..1761
                     /locus_tag="VFB80_07745"
     CDS             931..1761
                     /locus_tag="VFB80_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN33696.1"
                     /translation="MQLLDFVWLCLSAAAAGGINALAGGGTLLTFPTLIGVLSRQSGS
                     MAREAGVFANGTSTVALVPASLGSAWGFRRELFALRKLLIWLVPPSLVGAAIGVALVT
                     RLPPQYFDALVPWLILTAATLFVLQPLLVKRKPVVVLEDGKPAATESGLASVTPLGLA
                     GMVALQFCIAIYGGYFGAGIGILMLSGLGLMGLKNIHEMNGLKAVLGLAINGVAAAGF
                     VWEQKIHWPFALAMMGTSTVGGYVVAQFSRKIPGAYVRYFVIVVGFSLAAYYFWQRLR
                     "
     gene            2040..3113
                     /locus_tag="VFB80_07750"
     CDS             2040..3113
                     /locus_tag="VFB80_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33697.1"
                     /translation="MAKSSSADDVKLATNYEGLRETLRESRYADRLDRPLAFWALPSD
                     RRLPTALLARTLRELFTQSFDQLAATAGIGRKKLEMFIKLLVRATKEESAAKVEDYLP
                     GADETPADGLDDEGKFDPLRVSDMVWTKWRDTVRRHGVGGEKLGKLTPSLQSLPSVVW
                     STPLSFYLDLTLAEIRALKTHGEKRVAVVLEVFHTIHEMLDHIDPQGDLAVRLAPRTI
                     VAAEEWMAEARGRSFPPKREEIEQNMIEPIIQQLGIDAGDTVAKLARGRLGIGSEAES
                     VRNQSKALGVTRARVYQMLEECHNVMSIRWPDGRRQLDEFAQWLDENYASAESANLLA
                     SYRELLYPLKFDSVAETLRAEAS"
     gene            complement(3121..4425)
                     /locus_tag="VFB80_07755"
     CDS             complement(3121..4425)
                     /locus_tag="VFB80_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33698.1"
                     /translation="MTTHQYCDGIRRRDLLRVGLLGAAGIGLCDYLRLAAAGEIKPAR
                     ANAAIFVNLAGGPSHLDSFDPKPDAPAEYRGEFRTIPTNVPGIELCEHLPKLARCADK
                     FALLRGVSHSLTEHQMGTKYLNTGNRPIPSLEFPGYGAVVSKELESPKDLPPFVAIPK
                     TPQVAGVLGVEYAPFSTQATPRAGQPFTVRGITIGRGLSIEKIERRRKLLTDIEESFR
                     AYEKDSNLLQGLDRFSQRAHDILSSPRASLAFDVSREPASIAGHFPDNPLAQSCLLAL
                     RLVEAGVRFVSIDHGGWDTHDNIFPQMKDKLLPELDGGLAGLLAALELKGLLGTTAVM
                     VTGEFGRTPKISAQRAGRDHYPRAMFVLLAGGGMKPGQVVGASDARGEAPADGKGMSP
                     DDVAASFYHCLGIDAAKEYRTPTGRPVAIVRHGTVIRDLFGG"
     gene            complement(4438..>5973)
                     /locus_tag="VFB80_07760"
     CDS             complement(4438..>5973)
                     /locus_tag="VFB80_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN33699.1"
                     /translation="KIEELLARTTYVARWTTKLCEITGNSPRHFQDQAPPDEYSRNWY
                     EWIARRVRENVPYDELAAGIVLGISREPGRSYQDFIQEESAYYRAQEPADFTARATMP
                     YYWAKHTSRTPEERALNFSYAFLGVRIECAQCHKHPFDRWTKDDFQSFTALFERVGFG
                     VAEDGKQAHQELLNKLADKGNQAQRVRARLTRAQNGEIVPWQEVFLSRAGTRVEKGKV
                     VKIAEPITPHVLGGAEVGLQTDDDPRQALVSWMRRKDNPYFARVFVNRVWAEYFGVGI
                     IQPPDDLNLANPPSNAALLEYLTEAFIQHGFDMRWLHREIVSSRAYQRSLAANDTNRL
                     DERNFSRAVARRLPADALFDAIAQATCSSADVARATSDVAERAIGPKGGALIGRYGYG
                     NYASTVFGRSPRETNCDCSASNEPNLLQAIYTQNDKDVLAAIDRKGGWLNEIRPQLVS
                     GSAGERAALVKEAFLRTLSRAPTAKEAQLSESHLAEVGDRAEGFQELVWALLNSREFL
                     TNH"
BASE COUNT         1001 a         1881 c         1946 g         1145 t
ORIGIN      
        1 gccctggtat cagattccgc cgctcgattt cgtttccgag aacatggtgg cccgcgagat
       61 tcgcgacgag cggaacgttg acgtgcagct ggtgccggag gagatcgtgc cgcagcagaa
      121 gctgctcgat cgggcgcagg cgctgcgcga tggcgcgatg gccggctacg tcacgccgct
      181 gctgaatggc ggccccacgg cgcctccgag ccgcgagcct ccgccccaca tcggcgtgcc
      241 cggacaagga ttgcccggcg gtcagccgct gccctacgag ccgctgccga atccgggcgg
      301 accttagtcg cgcttcggtc attgcgacag ccaggcgatt tggcccgtgt ggtgccggcc
      361 gtgatgggca tacagcccca ggacttcggc cagccggaat tccttttgat attcgggatg
      421 aaagtaggag cgttggaagt cggcgtcgct catggctcgc agcagcagca cccagcgagc
      481 gtgcaaggct tccagcaact ggagcgagag cgtcacgtcg gcggatttcg agtcgggcag
      541 ctccgcccac cgcgtttcat cgtagggctt gatagtcggc cgctcctcgg tcaatgtcag
      601 ccggaaacga acgaatgcgt tcatgtggct atcggccagg tgatggacga tctgccggac
      661 gttccagttt ttgtagcgcc gctcgagttg gcccgccggc aggccgccga cgagcgagcg
      721 caacctcgcc ggcaattgct cgatctcggc gatcagctcg ccgcgacggg cgggcgtggg
      781 atgcggctcc gcgacatagg ggcccgcggg taagtctggc ggcgacatag gcacctcggc
      841 tgtgatccta atcgccggcc agagggcttg ccacgagtcg cgattgcagt cgtaatggaa
      901 acctgccgca gcacacctcg ccgctggatc atgcaactct tggacttcgt ctggctctgt
      961 ctttcggccg ccgccgcggg cgggatcaac gctctggccg gaggcggaac gctgcttacc
     1021 tttccgacgc tgatcggcgt gcttagccgg caatcaggaa gcatggcccg cgaggcgggc
     1081 gtttttgcca acggcacgag caccgtggcg ctagtgcccg cgtcgctcgg cagcgcctgg
     1141 ggctttcgcc gcgaattgtt cgcgctgcgg aaactgctga tctggcttgt gccgcccagc
     1201 ctggtgggag cggccatcgg cgtggcgctc gtcacgcgtt tgccgccgca gtatttcgac
     1261 gccttggtcc cctggctgat tctcactgct gcgacgctgt ttgtcttgca gccgctgctg
     1321 gtgaagcgca agcccgtggt cgtgttagaa gacggaaagc cggcagcaac ggaaagcgga
     1381 ctagcaagcg ttacgccgct gggactggcc ggcatggtcg cacttcagtt ttgcatcgcc
     1441 atttacggcg gctacttcgg cgcgggcatc ggcattctca tgctcagcgg cctggggctg
     1501 atgggtctga agaacatcca tgagatgaac ggcctcaagg cggtgctcgg tcttgcgatt
     1561 aacggcgtcg ccgcggctgg cttcgtgtgg gagcagaaaa tccattggcc gttcgctctc
     1621 gcaatgatgg gtacgtccac cgtcggcggg tatgtagtgg ctcagttcag ccgcaagatt
     1681 cccggcgcgt acgttcgtta ctttgtcatc gtggtggggt tttccctggc agcttattac
     1741 ttttggcagc gtctgcgttg acaaacctgc ccaactgtcc gacaagaggc ggatttttct
     1801 gatgaattag ctgcggcgac ttttctggct cttcgcgcaa taaatgggta atttctggta
     1861 aggaaaggaa gaattccagc cacaaagcag tttcgcaaga agcgtgcggc tggccccgta
     1921 acagaatctc caaagaattc gctttccacg cacttgactg agtttacgtg cagtggagaa
     1981 tggttgcagg ctgagaactg tcggaaaact gttgacactc tttcggagca acggttgtca
     2041 tggccaaatc gtcttctgcc gacgatgtga agctcgcgac gaactacgag gggctgcggg
     2101 agacgctccg cgagagccgc tacgccgacc gtttggatcg accgctcgcc ttttgggcgc
     2161 ttcccagcga ccgtcgcttg ccgacggcct tgctggcccg cacgcttcgc gagttgttta
     2221 cccagtcctt cgatcaactg gcggcgacgg ccggcatcgg tcgcaagaag ttggagatgt
     2281 tcatcaagct gctcgtgcgc gcaacaaaag aggaatccgc cgccaaggtg gaagactacc
     2341 tgcctggcgc ggatgagacg ccagccgatg ggctggatga cgaaggaaag ttcgacccac
     2401 tccgcgtctc cgacatggtc tggacgaagt ggcgcgatac ggttcgccgc cacggagtgg
     2461 gtggcgaaaa gctcggcaag ttgactccgt cgctgcagtc gctgccgtcc gtggtttgga
     2521 gcacgccgct gagcttctat ctcgacctga cgctggcgga aatccgggcc ctgaagacgc
     2581 acggcgaaaa gcgcgtggcc gtcgtgctcg aggtcttcca cacgatccac gagatgctgg
     2641 atcacatcga tccgcagggc gacctcgcgg tgcggctcgc gccgcggacg atcgtcgcgg
     2701 ccgaagaatg gatggccgaa gcccgcggcc gttcgttccc gcctaagcgc gaagagatcg
     2761 agcagaatat gattgagccg atcatccagc agctggggat cgacgccggt gacaccgtgg
     2821 ccaagctggc ccgcggacgg ctggggatcg ggtctgaggc cgaatcggtc cgcaatcaga
     2881 gcaaggccct cggcgtgacg cgggcccgcg tctatcagat gctcgaggaa tgccacaacg
     2941 tgatgagcat ccgctggccc gacggccgcc ggcagctcga cgagttcgcc cagtggctcg
     3001 acgagaacta cgcctcggcc gaatcggcca acctcctggc cagctaccgc gagctgctct
     3061 acccgctcaa gttcgattcc gtggccgaaa cgctgcgggc cgaagcttcg tagcggcgct
     3121 ctagccgccg aacaagtcgc gaattacagt cccgtggcgc acgatggcca cgggtcgtcc
     3181 ggtcggcgtg cgatactcct tagccgcgtc gattcccagg cagtgataga acgaggccgc
     3241 gacgtcgtcg ggcgacatgc ctttgccgtc ggcgggtgct tcgccacggg cgtcgctggc
     3301 tcccaccact tgcccgggct tcattccgcc gccggccagc agcacgaaca tcgcccgcgg
     3361 atagtggtcg cggccggccc gctgggcgct gatcttcggc gtgcggccga attcgccggt
     3421 gaccataacg gctgtcgtgc ccagcagccc cttgagctcc agcgccgcca gcaggcccgc
     3481 caggccgccg tcgagctcgg gcagcagctt gtccttcatt tgcgggaaga tgttgtcgtg
     3541 agtgtcccag ccgccgtggt cgatgctcac gaaccgcacg cccgcttcga ccaatcgcag
     3601 ggctaagagg cagctttggg ccagcggatt gtctggaaag tgcccggcga tcgacgccgg
     3661 ctcccggctg acatcgaagg ccaggctggc gcggggcgag ctgaggatgt cgtgggcccg
     3721 ctgcgaaaag cggtccagcc cctgcagcag gttgctgtcc ttttcgtaag cccggaagct
     3781 ctcttcgatg tccgtcagca gttttcgtcg ccgctcgatc ttttcgatcg acaggccgcg
     3841 gccgatggta attccccgaa cggtgaaggg ctggcccgcc cgcggcgtag cctgcgtgct
     3901 gaagggagca tattcaactc ccagcacgcc ggcgacctgc ggcgtcttgg gaatcgccac
     3961 aaacggcggc aggtccttcg gactctccag ttccttgctg acgaccgcgc catagccggg
     4021 gaattcgagc gaaggaatcg gccggttccc cgtgttgaga tacttggtgc ccatctggtg
     4081 ctccgtgagg ctgtggctga cgccacggag cagggcgaat ttgtcggcgc agcgggccaa
     4141 tttaggcagg tgctcgcaga gttcgattcc cggaacattc gtcgggatcg tccggaactc
     4201 gccgcggtat tccgccggcg catcgggctt gggatcgaag ctgtccagat ggctgggacc
     4261 gccagcgaga ttgacgaaga tcgccgcgtt ggcccgggcc ggcttgatct cgcccgccgc
     4321 ggccagccgc aaatagtcgc acaggccgat gcccgccgcg cccagcaggc ccactcgcag
     4381 caagtcgcgc cgccggattc catcgcaata ttggtgcgtc gtcataaatc gttccaccta
     4441 gtgattggtc aggaactccc gcgagttcag tagcgcccag accaactcct gaaagccctc
     4501 ggcgcgatcg cctacttcgg cgaggtgcga ctcgcttagt tgggcctcct tggcggttgg
     4561 tgctcgcgag agcgtgcgca gaaaggcctc cttgacgagc gcagcgcgct cgccggcgga
     4621 accgctgacc agctgcggac gaatttcgtt cagccagcct cctttgcggt cgattgccgc
     4681 cagaacgtcc ttgtcgtttt gcgtgtagat cgcctgcagc aggttcggct cattcgaggc
     4741 gctgcagtcg cagttcgtct cgcgcggcga gcggccgaag accgtggacg catagttccc
     4801 gtagccgtag cgtccgatca acgcgccccc tttgggcccg atcgcccgtt ctgccacgtc
     4861 gctggtcgcg cgggccacgt cggccgacga gcaggtcgcc tgggcgatgg catcgaacag
     4921 tgcgtccgcc ggcaggcggc gggcaacggc ccggctgaag tttcgctcgt ccagccgatt
     4981 cgtgtcgtta gcggccagcg atcgctgata ggcccggctg ctcacgatct cccggtgcag
     5041 ccatctcatg tcgaagccgt gctggatgaa cgcctcggta aggtactcca gcagtgcggc
     5101 attgctgggc ggattggcga gattcaggtc gtcgggtggc tggatgatgc ccacgccaaa
     5161 atactcggcc cagacgcgat tgacgaatac gcgggcaaag taagggttgt ctttgcgtcg
     5221 catccagctc accagtgcct ggcgcgggtc gtcgtcagtc tgcagaccga cttccgcacc
     5281 gcccagcacg tgcggtgtaa tcggctcggc tatcttgaca accttgccct tttcgacccg
     5341 cgtgcccgcc cgcgatagaa acacttcctg ccacggcacg atctcgccgt tttgcgctcg
     5401 cgtcagcctg gcccgcaccc gctgagcctg attccccttg tcggcgagct tgttcagcag
     5461 ctcctgatgc gcctgcttgc cgtcctcggc cacgccgaag ccgacgcgct cgaacagggc
     5521 agtgaagctc tgaaagtcgt ccttcgtcca gcggtcgaag gggtgtttgt ggcactgcgc
     5581 gcattcgatc cgaacgccca ggaacgcgta gctgaaattg agggcccgct cctcgggcgt
     5641 gcgcgacgtg tgcttggccc agtaataggg catcgtcgcg cgggcggtga agtccgccgg
     5701 ctcctgcgcc cggtagtagg ccgactcctc ctggatgaag tcctgatagc tccgcccagg
     5761 ctcgcggctg atgccgagta cgataccggc cgccagctcg tcatagggca cgttttcgcg
     5821 gacgcggcgg gcgatccact cgtaccaatt ccgcgaatac tcgtcgggag gggcctggtc
     5881 ctggaaatgc cgcggggagt ttccggtaat ctcgcagagc ttcgtcgtcc agcgggcgac
     5941 ataggtggtc cgcgcgagca gctcctcgat ctt
//
LOCUS       DASRJB010000289         5042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2339110, whole genome shotgun sequence.
ACCESSION   DASRJB010000289 DASRJB010000000
VERSION     DASRJB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339110"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..412
                     /locus_tag="VFB80_07765"
     CDS             92..412
                     /locus_tag="VFB80_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33700.1"
                     /translation="MLTQEEAWIIAEEHAKAKGLPWENMLAFFEPDLMWNDSPRAAWS
                     FIDDGGRMGGNLQIAIDAETGDVLEVCSSADSGRLALGFKLNHYQLAVEQPRVGADIY
                     CGSP"
     gene            536..1690
                     /locus_tag="VFB80_07770"
     CDS             536..1690
                     /locus_tag="VFB80_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZN33701.1"
                     /translation="MSRLGGSLALPLALVVCLPGPGRADDALDEMPPASEAALERGLA
                     WLAKNQGVEGNWGSNDLGLVGMGALAFMSAGHAPGRGKYGREVEKALDFVISRSKPSG
                     LLNISDAQRDMYNHGLATFVLGQAHGMQTTKDRKLNRVLDSALKLIANTQCEDGGWDY
                     KAARQHAGHDLSLAVMQAKALRSAVDSGLEVPPEVISQAIQSVRDHYASRGGRGSSEA
                     EQQRLPGQFTYSKGGGGGTIAMAAAGVVCLQEFGQYDDWRIGKNMEVIQAAIQEIPRT
                     RARDGSMPFDAYTLYYVGQALYQVNGDYWKNNYPKLRDYLVDSQVADPSNPQNHGAWR
                     DHGARGGGRVGGKDGELYATSVACFILAIPNRYLPILQEGKIESFRKASQ"
     gene            1763..4315
                     /locus_tag="VFB80_07775"
     CDS             1763..4315
                     /locus_tag="VFB80_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN33702.1"
                     /translation="MGFLSFTAWQFAAVGAACAAGPLIIHLLNRRRYKVVKWAAMDFL
                     RQALQRNRRIMQIRDILLLVLRTAAVLLFGLALARPYFATREEEFDDRQPLHAVILID
                     NSLSMGYESLEGTLLDKAKDRARQLIDKLPAGSRMSIIPVCGSREGFSPDPYETKENA
                     AEALDKIELVDRSASLVRAANEAKRACEAAPELAKRIVFIGDQQELNWQGARQSEVLK
                     ELPAMQIVDVGPANWENTWIADLRVQDGLADIETPATIVVELAHRGSGPRRDLLVTLT
                     MGDPNSGGSIIGEKTVTLEPGLGTREVDFECVFNTLSELPEPNRPVFVPLKATITPDR
                     LAADDQRFLAVPVVAALPVVFVDQYGPEQEDPIKGRLGETRHLRKLLAPKTSRSDAPR
                     QLVNVRHITQDELTRDVLADARLLVIAGLREPGDMVPLLRDYVGQGGQLVIVAGADFD
                     PVAWNDAAWLGGAGVLPLPLAREALGETPEVAGANLKPFFLSFDSLAGEDYFHLAGVA
                     EPELRDLYAEPFFFKSVQVESSPDVLAAWKQAELKRLEEELTQEAARRQQQAAAAGEK
                     GDAAIAQPIPQPDARPNWLAWAAEAGENIEPVLPEEPAQRQRLLESLVQASAPRVLAR
                     YDLPGRPPFLVSRPIGRGEVLFCSTGLLSSWNTLPKTNAVLIFDRILRGLTQGTLPRR
                     NFAATERLALPLPPNEQNLVVTLARPGQKMGDEPLDVGYIGPEQRGVQVQGLFQRGIY
                     RVAGYRPSLSEQAPLTAEKPVWDLPLVVSGAAAESDLTALTRTQFEEAAQAANLRWIG
                     RGEEINLAGTAISGQNSWWWLVLLVLVLLLVEMSVLAWPTFQPAAPAPGAAT"
     gene            4312..>5042
                     /locus_tag="VFB80_07780"
     CDS             4312..>5042
                     /locus_tag="VFB80_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33703.1"
                     /translation="MIRALGWLLGIENATAIDRIDVSLAAPWAADSAFWVFLIAAAFI
                     AGSLIFYLKWQQKGPRGPRIALAVFRGILLALLLFTLADPILQLIVENKQAPFLYVIF
                     DGTDSMAIEDELPDAERAAIEKAVGYTPTRSTSEGVTLPKPANGKPSRIAYLQSLLRK
                     SDNNPFTKLTAAKKVQLEAFLFDGNTTSQLRKLNLSKTAGGAFDPAYLAEQLTTKGQV
                     TALGSVVHEVGQQFGSGRLAGVVLF"
BASE COUNT          891 a         1584 c         1741 g          826 t
ORIGIN      
        1 ggcggcatgg gcggccagca ttcagtgacg aggagcggaa gttgggcgag cggctgacga
       61 accacctgcc ggcaaagaag ggggctaggc tgtgctgaca caggaagagg cttggatcat
      121 tgccgaagag catgcgaagg ccaagggttt gccgtgggag aatatgcttg ccttcttcga
      181 gccagaccta atgtggaatg acagtccgcg agccgcttgg tctttcattg acgatggagg
      241 ccgtatgggt ggaaatctcc aaatcgccat cgacgccgag actggcgatg tgctggaagt
      301 ttgttcttca gccgatagcg gccggcttgc actgggtttc aaattgaacc attaccagtt
      361 ggcggttgaa cagccgcgag taggggcgga catttattgc ggctcgccgt aacgtattcg
      421 ctttcttgga cataggtgtc taggatgggt cttttctgcc gatcgttttg cgcactcgcg
      481 gcgcagcaag tgacgtcgta aggtgtttgg cgtgcgtatg ttacggaaga acctggtgag
      541 caggctcggt gggagcctcg ccctcccgtt ggcactggtc gtgtgcttgc caggtccggg
      601 cagggccgac gacgctttgg acgagatgcc gccggccagt gaagcggcgc tcgagcgcgg
      661 gctggcctgg ctggcgaaga accagggcgt ggaggggaac tggggctcga acgatctggg
      721 gctggtcggc atgggcgcgc tggcgttcat gtcggccggg catgcgccgg ggcgggggaa
      781 gtacggccgg gaggtcgaga aggccctcga tttcgtgatc agccgcagca agccgtcggg
      841 gctgctcaac atcagcgacg cccagcggga catgtacaac cacgggctgg cgacgtttgt
      901 cttgggccag gcccacggga tgcagaccac caaggaccgc aagctcaacc gggtgctcga
      961 cagcgcgctg aagctgatcg ccaacacgca gtgcgaggac ggcggctggg actacaaggc
     1021 cgcccggcag cacgcagggc acgacctcag cctggcggtg atgcaggcca aggccctgcg
     1081 gagcgcggtc gatagcggcc tggaagtgcc gcccgaagtc atcagccagg cgatccagag
     1141 cgtccgcgat cactatgcca gccgcggcgg gcgcggctcg tccgaagcgg agcagcagcg
     1201 gctgcccggg cagttcactt acagcaaagg gggcggcggg ggcacgatcg caatggccgc
     1261 ggcgggcgtc gtctgcctcc aggagttcgg ccagtacgac gactggcgga tcggcaagaa
     1321 catggaggtg atccaggccg ccattcagga gattccccgc acgcgggccc gcgacggcag
     1381 catgccgttc gatgcctaca cgctgtatta cgtcggccag gccctgtatc aggtgaacgg
     1441 cgattactgg aagaacaact atccgaagct ccgcgactat ctggtcgatt cgcaggtcgc
     1501 cgatccaagc aacccgcaga accacggcgc gtggcgcgac cacggggccc gcggcggcgg
     1561 ccgcgtcggc ggcaaggacg gcgagctcta cgccaccagc gtcgcctgct tcatcctggc
     1621 gatcccgaat cggtacttgc cgatccttca agagggaaag atcgagagtt ttcggaaagc
     1681 aagtcagtag tcagtggtca gtggtccgtt gggaaagaca ttctccaacg gaccactgac
     1741 cactgactac tgccaactga ctatgggttt tctctccttc accgcctggc aattcgcagc
     1801 cgtgggggcg gcgtgcgcgg cggggccgct gatcatccac ctgctcaatc ggcggcgcta
     1861 caaggtggtg aagtgggcgg cgatggactt cctgcggcag gcgctgcagc gcaatcgccg
     1921 catcatgcag attcgcgaca tcctgctgct cgtcctgcgg accgcggcgg tcctgctgtt
     1981 cggcctggcg ctagctaggc cgtactttgc gacacgggaa gaggagttcg acgaccggca
     2041 gcccctgcac gcggtgatcc tgatcgacaa cagcctgagc atgggctacg agtcgctcga
     2101 aggcacgctg ctcgacaagg ccaaagaccg ggcccgccag ctcatcgaca agctccctgc
     2161 cggcagccgg atgtcgatca ttccggtctg cggctcgcgc gagggcttca gccccgatcc
     2221 ttatgagacg aaggagaacg cggccgaagc cctggacaag atcgagctgg tcgatcgctc
     2281 ggccagcctg gtgcgggcgg ccaacgaagc caagcgggcc tgcgaagccg cgccggagct
     2341 ggccaagcga attgtgttca tcggcgacca gcaggagctg aactggcaag gcgcgcggca
     2401 gagcgaggtg ctcaaggagc tgccggcgat gcagatcgtc gatgtcggcc cggcgaattg
     2461 ggagaacacc tggattgccg accttcgcgt gcaggacggg ctggccgaca tcgaaacgcc
     2521 cgctacgatc gtcgtcgagc tggcccaccg cggcagcggc ccccgccgcg acttgctggt
     2581 gacgctgacg atgggcgatc cgaattccgg cggcagcatc atcggcgaga aaacggtgac
     2641 gctcgagccg ggcctcggca cgcgcgaggt cgatttcgag tgcgtcttta acacgctcag
     2701 cgagctgccc gagccgaacc ggccggtgtt cgtgccgctg aaagccacga ttactcccga
     2761 ccgcctcgcg gcggacgacc agcggttttt ggccgtgccc gtggtggcgg cgctgcccgt
     2821 cgtattcgtc gatcaatatg gacccgagca ggaggacccg atcaagggcc gcctcggcga
     2881 gactcggcac ctgcggaagc tgctcgcgcc caagaccagc cgcagtgacg ccccgcggca
     2941 gctcgtgaac gtccgccaca tcacgcagga cgagctgacg cgggacgtgc tggccgacgc
     3001 acggctgctg gtcatcgccg gcctgcgcga gccgggcgac atggtgccgc tgctcaggga
     3061 ttacgtgggg cagggcgggc agctcgtcat cgtggccggc gctgattttg acccggtcgc
     3121 ctggaacgac gcggcctggc tgggcggcgc gggagttctg ccgctgccgc tggcccgcga
     3181 agctttgggc gagacgccag aagtggccgg cgcaaatctg aagccgttct ttctctcgtt
     3241 cgacagcctg gcgggcgagg attattttca cctggccggc gtcgcggagc cggagctgcg
     3301 cgacctgtat gcggagccgt tcttcttcaa gtcggtgcag gttgaaagct cgcccgacgt
     3361 gctcgccgcc tggaagcagg cggagctgaa gcgcctggag gaagagctga cgcaagaggc
     3421 cgcccggcgg cagcagcaag cggccgcagc tggcgagaag ggcgatgcag cgatcgcgca
     3481 gccgattccg cagcccgatg cgcggcctaa ctggctggcc tgggccgccg aggccggcga
     3541 aaacattgag ccggtcctgc ccgaagagcc cgcgcagcgc cagcgcttgc tcgaatcgct
     3601 ggtccaggcg agcgcgccgc gggtgctggc ccgctacgac ttgcccggcc ggccgccgtt
     3661 tctggtttcg cgcccgatcg gccgcggcga agtgctgttc tgctcgacgg ggctcttgtc
     3721 gtcctggaat acgctgccca agaccaacgc ggtgctgatc ttcgaccgca tcctccgcgg
     3781 cctgacgcag ggcacgctgc cgcggcggaa tttcgcggcg accgagcggc tggcgctgcc
     3841 cttgccgccc aacgaacaga atctcgtcgt gacgctcgcc cgccccggcc agaaaatggg
     3901 cgatgagccg ctggatgtgg gctatatcgg cccggaacag cgaggcgtgc aggtgcaggg
     3961 cctgtttcag cgcgggattt accgcgtcgc cggctaccgg ccctcgcttt ccgaacaagc
     4021 gccgctcacg gcggagaagc ccgtctggga tttgccgctg gtggtcagcg gagcggcggc
     4081 cgagtcggac ctgacagcgc ttacgcggac gcaattcgag gaggcggcac aggcggccaa
     4141 tctccgctgg atcggccgcg gcgaagagat caacctggct ggcacggcaa tcagcgggca
     4201 aaactcgtgg tggtggctgg tgctgctggt gctcgtgctg ctgctggtcg agatgagcgt
     4261 gttggcctgg ccgacgtttc aaccggcggc tcccgcccca ggagccgcca catgatccgg
     4321 gcactcggct ggctgctggg catcgagaac gcgacggcga tcgaccggat cgacgtgtcg
     4381 ctggctgcgc cctgggccgc ggacagcgca ttttgggtct tcctgatcgc ggcggccttc
     4441 atcgccggct cgctgatctt ttatctgaag tggcagcaga aaggcccccg cggcccgcgg
     4501 atcgcactgg ccgtgtttcg cgggatactg ctcgcgctgc tgctgttcac gctggccgac
     4561 ccgatcctgc aactgatcgt cgagaacaag caagcgccgt ttctgtacgt gatcttcgac
     4621 ggcaccgaca gcatggcgat cgaggacgag ctgcccgacg ccgagcgggc cgccatcgag
     4681 aaggccgtcg gctacacacc aacccgaagc accagcgagg gagtcacgct gcccaagcca
     4741 gccaacggca agccttcccg catcgcctac ctgcagtccc tcctccggaa atccgacaac
     4801 aacccattca cgaaactcac cgccgcgaag aaggtgcagc tcgaagcctt cctgttcgac
     4861 ggcaacacca ccagccagct tcgcaagctg aatctgtcga agaccgcggg cggcgctttc
     4921 gatccggcct acctggctga gcagctgacg acgaaagggc aagtcacagc gctcggctcg
     4981 gtggttcacg aagtcggcca gcaattcggc tccggccggc tggcgggcgt cgtgctgttc
     5041 ag
//
LOCUS       DASRJB010000290         9579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2339471, whole genome shotgun sequence.
ACCESSION   DASRJB010000290 DASRJB010000000
VERSION     DASRJB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339471"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFB80_07785"
     CDS             complement(<1..818)
                     /locus_tag="VFB80_07785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33704.1"
                     /translation="MPQAISAEQLASRMLDAGLIEQRQVEGLWAELGTRDVPFGDFSS
                     LLLRKELLTNYQLERLLKGEKGGYFYGDYKVLYLVGTGTFARVYRAVHLESGRIVAIK
                     ALRKRFRDDRSMNEQFVREGKIGMQLRHPNIVPIYEVIEQPIPCLVMEFVEGRNLREF
                     TRVRKKLSPVESMRVTVDILSALTYAATLGMTHRDLKMSNVLITSRGKGKLVDFGLAG
                     LQSLRANAANAEETNPRTIDYAGLERASGVRNNDPRSDLFFVGVILYNMCTGVSP"
     gene            complement(1084..1377)
                     /locus_tag="VFB80_07790"
     CDS             complement(1084..1377)
                     /locus_tag="VFB80_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZN33705.1"
                     /translation="MSLPNATQPTEELPKDVADLAAAVALLPAEYRSALEPLLFRVVD
                     STKRRRRILNLVQEALSQLRLDMKYLVFDLEATRRERDALREGPDGEQHRGDT"
     gene            1659..4304
                     /locus_tag="VFB80_07795"
     CDS             1659..4304
                     /locus_tag="VFB80_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33706.1"
                     /translation="MSRAIRRAACCLTLWLLLSATAAAQLRNSFETQQCSWMLRGANC
                     GVRELLHERSPREARSGQSSEHLRLEVGSGTFVYLVQPIGRAPLIQEFRPSLFVKADR
                     ASVQLLARVVFPRSIDRGSGQPITTFLRGEFYTDVGNWQQLAINGAARLLDDETRTLR
                     TQFGDVDPREAYVDLLVLNAYTAPGPIELWIDDLEIDGYVNLDETAGQASARTPTIAG
                     DAGQAEPPPAAASVQGSLLLVSSRPLMPRIAVQRGEPLEWLKSLGFNTIKLGSSPSPA
                     ELNEARRLGLWLVAPPPYADPRAAPQAYEPVIAWSLGTRLSEPDFGPTRDLIREIRGL
                     DPQQDRPLLAGVESGLSEHSRLANLLLLERPLLGTTYELADWRQWLLLRPRLAQPGVP
                     LLASIETQRPARLSEQLLLFSRGQPVEEDVDPQQIRLAAYQAIAAGVRGLVFPSEQPL
                     AIDTGPAALRTDALKLLNMELRLLEPWIAAGQLVEELPAESGGTQLSVLQTERSRLLI
                     VTQHAPAQQYALGPPPRNTLSLLVPGIGISDQAHLVSLAGIKPLKLTHTSSGGRITLE
                     DASHAAAVAITQDPLALLHLHRTHARFKQEACRLRYDLAARRYIRTLEIDQALVAAGH
                     PLASGAASLREAFGQLEQARKLFESSDFERSHAATDKAEYALARVRRGHWEQTAAAFS
                     SPASSPCLAQFTALPLHWRFAEQIQKAAWGPNVQVGGDMEALDQMIQAGWQQRRAAPS
                     GVNADVSLSLSEPHAGRSALRLQAWPADAQRAPRLVERPTVWITSSPVPVREGQLVRI
                     RGWAQVPRPVVGSDEGLLIFDSLGGPDLGDRIRLTQGWREFTFYRAVPRGGQLSVTFA
                     LTGLGEAALDDLQVSLLDPPPLRPQ"
     gene            complement(4321..4548)
                     /locus_tag="VFB80_07800"
     CDS             complement(4321..4548)
                     /locus_tag="VFB80_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33707.1"
                     /translation="MSQTFAQLGLAAVHGLRESILIRGGAYCGRRFDSAAGHAIWFLE
                     ENQIKFYDAAGRLAEVAETSAPERTSQRAAA"
     gene            4849..5040
                     /locus_tag="VFB80_07805"
     CDS             4849..5040
                     /locus_tag="VFB80_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33708.1"
                     /translation="MDDDFEVELQDLAIDEIRELLAEAGAQITTEQAAQLARLVAQAG
                     GIQEALAALAQLTQQREAA"
     gene            complement(5045..5362)
                     /locus_tag="VFB80_07810"
     CDS             complement(5045..5362)
                     /locus_tag="VFB80_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33709.1"
                     /translation="MAKKSTSGTKSASIRNYKSDHPSAKPKEIAEALSKSGAKVTPAF
                     VSTVLSNDKRRGKKRKGGRKPGRPAAAGGFESLVQAKKLADQMGGVAKAKAALDALAK
                     LLG"
     gene            complement(5538..6848)
                     /locus_tag="VFB80_07815"
     CDS             complement(5538..6848)
                     /locus_tag="VFB80_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33710.1"
                     /translation="MITALVLWSGLALAQVAEAPPATADAAAQLKSKVAALVRQLDLD
                     EAAKRDAAEKELIALGPEVAPLLPPISNRTPAEISIRLNRVRNALTKLEIEAATKPSQ
                     VTLSGEMPVSKAFAEITRQTGNKLIDYRERFGQEASDPTIKVELDKVPFWQALDTVLD
                     AAKLTLYNFDEEQGGVAYTSRGSDAGPRTGRASYSGLFRLEPSRIEATRDLKNSTMHG
                     VKLTADALWEPRVRPIVLEVPLAEVSAKDEAGKEIGIDQTEGTLEVPVEGTNAAVEIE
                     LPLTAPERSVKTVASIKGKITAVVLGKIETFEFADVDKIERAKAAELERGGVTVVLES
                     CRKNGDIYDVSMRVRFDRAANALESHRSWIYNNECYMLDPKGTRIENAGLEANLLAVN
                     EVGLSYKFDIGEDAKPAGYKFVYRTPAAIIKIPVDFELKGIDLP"
     gene            6974..8065
                     /locus_tag="VFB80_07820"
     CDS             6974..8065
                     /locus_tag="VFB80_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33711.1"
                     /translation="MPLRVTCPAGHKLIVPDDRAGKTLRCPRCSAAFDVPGERVQLSG
                     LRVQEAGVGGRESEATRVAVVAQLPIVQAQVESPPVVKPKSRPRPPAPAIVQAAPPAV
                     LVAPEPKPLQAIALAPPPPQPAVEPAPIRVREEPVAEEPVAKVAPPPVVESPPVLPEA
                     PVPSSPQTESSEIAPTANAPAARAPSEAEVPEQVESPAPAPQAVVPDRRWLPAAYSLA
                     AGLIAAALFGAAPAIWDVVDYVRYYELVDSPHVARWALVLLLLGLVQIAYALFLFQLP
                     DWASVWVVTLYSLGLAGLYALMLGLVLISREDGLLVGQHGLQLADKLAGGRAALWCLA
                     MTSVFTILAFFAGRLSVQWRRAESARLAA"
     gene            8244..8840
                     /locus_tag="VFB80_07825"
     CDS             8244..8840
                     /locus_tag="VFB80_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33712.1"
                     /translation="MTSLPETRASLILRLPDGADVQAWDEFAAIYGPLVYRLARRQGL
                     QPADADDLVQEVLSAVARSVEGWLHNGQRGPFRAWLLRIAKNAAINVLTRKRHQPLAA
                     GGHDLLLAQTADPAGELSGEFDLEYRREVFRWAATQLRDLVADSTWQAFWRTSVEEQP
                     IERVAQELKMSVGSVYIARSRVMARLRELARNFGENER"
     gene            8837..>9579
                     /locus_tag="VFB80_07830"
     CDS             8837..>9579
                     /locus_tag="VFB80_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33713.1"
                     /translation="MKAAAQPCDESRLVVLLRGNEDSADFAAAQGHVESCAACQQRLS
                     TLAAEAGEWEETRELLRESEGDAALTEERKNRPWNVAGRRPTNWTEALAKQLLAPPSH
                     PEMLGRLGRYEIERLIGSGGMGIVFKGFDTELNRPVAIKVLAPHLASSGAARQRFARE
                     ARAAAAVVHEHVVAIHNVETGGDAPFLVMQYVAGESLQARLDRQGPLELCEALRIAHQ
                     TAAGLAAAHAQGLVHRDVKPSNILLEQGL"
BASE COUNT         1560 a         3204 c         3152 g         1663 t
ORIGIN      
        1 ggcgacacgc cggtgcacat gttgtagaga atgacgccga cgaagaagag gtcgctgcgg
       61 gggtcgttgt tccgcacgcc gctggctcgc tccaggccgg catagtcgat cgtccgcggg
      121 ttggtctctt cagcgttggc cgcattcgcc ctcagcgact gcagccccgc caggccaaaa
      181 tccaccagct tgcccttgcc gcggctggtg atcagcacgt tcgacatctt caggtcgcgg
      241 tgggtcatgc ccagcgtggc ggcgtacgtc agggccgaaa gaatatcgac cgtgactcgc
      301 atcgactcga ccggcgagag cttcttgcgg acccgcgtga actcgcgcag gttccgcccc
      361 tcgacgaatt ccatcacgag gcagggaatc ggttgctcga tgacctcgta aatcggcacg
      421 atgtttggat ggcggagctg catgccgatc ttgccttcgc ggacgaactg ctcgttcatc
      481 gagcgatcgt cgcggaaccg cttgcgcagg gccttgatcg ccacgatgcg gcccgactcc
      541 agatgcacgg cccgataaac gcgggcgaaa gtgcccgtgc caaccaggta cagcacctta
      601 taatcgccgt agaagtagcc tcccttttcc cccttgagca gccgctcgag ctgatagttc
      661 gtgagcagct ctttgcgcag caggaggctg gagaagtcgc cgaacggcac gtcccgagtg
      721 cccagttcgg cccacagtcc ctcgacctgc cgctgttcga tcagaccggc gtccagcatt
      781 cgagacgcaa gttgttccgc gctgatggct tgaggcatgc tcgcttgccg cacggacgcg
      841 ccccaatcgg cgcggcgcgg accacccttt ttcccagcgc cggttctgaa ccaacactgg
      901 tgaggaagaa actccagcgg cgtccatcga ggcccatttt ggccgacgcc gcccgaccgc
      961 cttatgctat tccaggccgc ggcgggccgc aatcctgcgg aggacgcaac gaaacgcccg
     1021 ccctccgggg cactggaggg cgggcgtcgc tgaacaagga gtctcgaatt attcaggagt
     1081 tgtttaagta tcaccgcgat gctgctcgcc atccgggcct tcccgcaggg cgtcacgctc
     1141 gcgccgcgtc gcttccaggt cgaacaccag gtacttcata tccagccgca actggctgag
     1201 ggcctcctgc accaggttca aaatccgccg ccggcgcttc gtgctatcca cgacgcgaaa
     1261 caagagcggc tccagcgccg agcgatattc cgccggcaac agcgccacgg ctgcggccag
     1321 atcggcgacg tctttgggaa gctcctcggt cggctgagtg gcgttgggca agctcatgcg
     1381 gcggcggctc cggggtgttt ggcgagcgtg cagtagttgg aaagcacccg gcatgccgcc
     1441 gccccgactg cgattccgcg gcgctctcgt aagtccttac gaggcaaata tttgcgacga
     1501 atcccaagca gcctccccgg aggcgccagt cgttgcacgc ctgccacgcc tgctctggag
     1561 cgtgaacgta aatcctgata caagccccgt ttacgtcaaa agcaacagac tggccacgcg
     1621 ttgccgtttg gcaacgccga gcgacgataa acctagagat gtcgcgtgcg atccgaagag
     1681 ccgcctgctg cctgacgctc tggcttctgc tgagcgcgac ggctgccgct cagctgcgca
     1741 actcgttcga gacccagcag tgctcctgga tgctgcgcgg ggcgaattgc ggcgtgcgtg
     1801 agctgttgca cgagcgctcg ccgcgcgaag cccgctccgg gcaatcgagc gagcacctgc
     1861 ggctggaagt cggcagcggc acgttcgtgt atctcgtgca gccgatcggc cgtgcgccgc
     1921 tcattcagga atttcgcccg tcgctgttcg tcaaggccga tcgggcgagc gtgcagctcc
     1981 tggcccgcgt agtctttcca cggagcatcg accgcggcag cgggcaaccg atcaccacgt
     2041 ttctccgcgg cgagttctac accgacgtcg gtaactggca gcagctggcg attaacggcg
     2101 cggcccgcct gctcgacgac gagacccgca cgctgcgtac gcaattcggc gacgtcgatc
     2161 cgcgcgaagc ctacgtcgat ttgctcgtgc tcaacgccta caccgccccc gggccgatcg
     2221 agctgtggat cgacgatctg gagatcgacg ggtacgtcaa cctggacgag accgcgggac
     2281 aggccagtgc gcggactcca acgatcgccg gcgacgcggg tcaggccgag cctcctccag
     2341 ccgccgccag cgtgcagggc tcgctccttc tggtcagcag ccggccgctg atgccgagga
     2401 tcgccgtgca acgcggcgag ccgctggaat ggctgaagtc gctgggcttc aatacgatca
     2461 agctcggctc atcgccgtcg ccggccgaat tgaatgaggc ccgccggctg ggattgtggc
     2521 tcgtcgcgcc gcccccctac gccgatccgc gcgccgcgcc gcaggcttat gagccggtca
     2581 tcgcctggag cctgggtacg cggctgtcgg agccggattt cggcccgacg cgcgacctga
     2641 tccgcgagat tcgtgggctc gacccgcaac aagaccggcc gctgctcgcg ggagtcgaaa
     2701 gcggattgag tgagcacagc cggctggcca atttgctgct gctcgagcgc ccgctgctgg
     2761 gcacgacgta cgagctggcc gattggcggc agtggctgct tctccgtccg cggctggcac
     2821 agcccggcgt gcctctgctg gcgagcatcg aaacgcagcg gccggcccgg ctgagcgagc
     2881 agctactgct cttcagccgt gggcagcccg tcgaagagga cgtcgatccg cagcagatcc
     2941 ggctggcggc ctatcaggcg atcgctgcgg gggtgcgcgg cctggtcttt ccgtccgagc
     3001 agccgctggc catcgacacg ggcccggcgg cgctgcggac cgacgcgctc aagctgctca
     3061 atatggaatt gaggctgctg gagccctgga ttgccgcggg gcagctagtc gaggagcttc
     3121 cggccgagag tgggggcacg cagttgagcg tgctgcagac cgagcgctcg cggcttttaa
     3181 tcgtgacgca gcacgccccg gcgcagcagt acgcgctcgg cccgccgccg cggaatacgc
     3241 tctcgctgct ggtccccggc atcggcatct cggaccaggc gcacctggtt tcgctggcgg
     3301 gaatcaagcc gctgaagttg acccacacca gcagcggcgg gcggatcacg ctggaggacg
     3361 cttcgcatgc ggcggccgtc gccatcacgc aggacccgct ggcgctgctc cacctgcacc
     3421 gcacgcacgc caggttcaag caggaggcct gccgcctgcg ctacgacctg gcggcgcggc
     3481 ggtacatccg cacgctggag atcgaccagg cgctagtcgc ggccggccac ccgctggcga
     3541 gcggcgccgc gtcgctgcgc gaggcctttg ggcagctcga gcaagcccgc aagttgttcg
     3601 agtccagcga cttcgagcgc tcgcacgcgg cgaccgacaa agcggagtac gcgctggctc
     3661 gcgtccgccg cggacactgg gagcagacgg cggcggcgtt ttcatcgcct gcatccagtc
     3721 cctgcctggc gcagttcacc gcgctgccgc tgcattggcg cttcgccgag caaatccaga
     3781 aggccgcctg ggggccgaac gtccaggtcg gcggcgacat ggaggccctc gaccagatga
     3841 ttcaggccgg ctggcagcag cggcgggccg cccccagcgg ggtcaacgcc gacgtcagcc
     3901 tgtcgctcag cgagccgcac gcgggccgca gcgctttgcg gctgcaagcc tggcccgccg
     3961 acgcgcagcg agcgccgcgg ctggtcgagc ggccgaccgt gtggatcacg tccagcccgg
     4021 tgcccgtccg cgaggggcag ttggtgcgga ttcgcggctg ggcccaggtt ccgcggccgg
     4081 tggtgggcag cgacgaaggc ttgttgatct tcgattcgct gggagggccg gacctgggcg
     4141 accgcatccg cctgacgcaa ggctggcgcg agttcacgtt ctatcgcgcc gtgccgcgcg
     4201 gcggccaatt gtcggtcacg ttcgccttga cgggtctggg cgaagcggcc ctcgacgacc
     4261 tgcaagtctc gctgctggat cccccgccgc ttcgtcccca gtgacgctgc gaatcgattg
     4321 tcaggcggcc gcccgctggc tggtccgctc tggcgcgctg gtctcggcga cttcggccag
     4381 ccgaccggct gcgtcgtaga acttgatctg gttctcttcc aggaaccaga tggcgtgtcc
     4441 cgccgcgctg tcgaaccggc ggccgcaata ggcgccgccg cgaatcagga tcgactcgcg
     4501 caagccgtgg actgctgcca gccccagctg agcaaaagtc tgggacacac gatgtcgtac
     4561 cgcttccgtc aattgggcgt gaggcatacc gggtgacgat agcaaggtgc gatgcgccgg
     4621 gtagaagcgc ccggctgccc cgattcgtgg gggctgccgg ccttgaaatg catcggccga
     4681 aaattcgccg ctaattgccc tcgccgtcct ccaccgcgtc atgttccgcg aactgcgcgg
     4741 cttgtgagat cggaattgtc ggcgcgcccg tggcggcaaa agcggcggct gccgaactaa
     4801 ctttgagacg gcaggccact ctcctattca ccgctgctag caaccactat ggacgacgac
     4861 tttgaagtcg agctgcaaga cctggccatc gatgagatcc gcgagctact agccgaagcc
     4921 ggcgcgcaaa tcaccaccga gcaggccgcg caactggccc gcctcgtcgc gcaggcgggc
     4981 ggcattcagg aagcgctcgc cgcgctggcc cagctcaccc agcagcgcga ggcagcgtga
     5041 agcgctagcc cagcagtttt gccagggcgt cgagcgcggc cttggccttg gcgacgccgc
     5101 ccatctgatc ggcgagcttc ttggcctgga ccaggctctc gaagccgccc gcggccgccg
     5161 gccggccggg tttgcgtccg cccttgcgct tcttgccgcg gcgcttgtcg ttagagagga
     5221 ccgtgctcac gaaggcaggc gtgaccttcg ccccggactt gctcaatgct tcggcgattt
     5281 ccttcggctt ggcgctggga tggtcggact tgtaattgcg aatcgacgcc gacttggtgc
     5341 cgctggtgga cttctttgcc attgcgcgat ggtcctcaag gagagtagat ggtgctgaac
     5401 atgagacgac aaaagtagcc agccagctag ctgatgtcaa ccaatgcgcg caataggaga
     5461 ctcactataa gtctcacagc cacccaaaac agctacattc aaccttagaa agataccgca
     5521 ctcgagccta aaggctgcta cggtagatcg attcccttca attcaaaatc cacgggaatc
     5581 ttgataatgg cggcgggcgt gcgatagaca aacttatagc ccgcgggctt cgcgtcttcg
     5641 ccgatgtcga acttataaga cagcccgact tcattgaccg ccagcaaatt ggcttccagc
     5701 ccggcgtttt cgatccgggt gcccttgggg tcgagcatgt aacactcgtt gttatagatc
     5761 cagctgcggt gcgactccag cgcgttggcg gcgcggtcga agcggacgcg catcgaaacg
     5821 tcgtagatat cgccgttctt gcggcagctt tccaggacca cggtcacgcc accccgctcc
     5881 agctccgccg ccttggcccg ctcgatcttg tccacgtcgg cgaattcgaa cgtctcgatc
     5941 ttgcccagga caaccgccgt aatcttgccc ttgatcgaag ccacggtctt tacgctccgc
     6001 tccggcgcgg tgagcggcag ctcgatctcg acggccgcgt tcgttccttc caccgggact
     6061 tccagcgtcc cttccgtttg atcgatgccg atctccttgc cggcctcgtc cttggcggaa
     6121 acttcggcca gcggcacttc cagcacgatc ggccgcaccc gcggctccca cagtgcgtcg
     6181 gccgtgagct tcacgccgtg catcgtggag ttcttcaagt cgcgcgtcgc ttcgatccgc
     6241 gacggttcca gccggaacag gccgctataa ctggcccgtc cggtgcgcgg cccagcgtca
     6301 ctgccgcggg acgtataggc tacgccgccc tgctcttcgt cgaagttata gagcgtgagc
     6361 ttggcggcat cgagcaccgt gtcgagcgcc tgccagaacg gcaccttatc cagctcgacc
     6421 ttgatcgtcg gatccgaggc ttcctggccg aaccgctcgc ggtaatcgat cagcttgttg
     6481 ccggtctggc gcgtgatctc cgcgaacgcc ttggaaaccg gcatctcacc ggacagcgtc
     6541 acctgcgaag gcttcgtggc ggcttcgatt tccagcttcg tcagcgcgtt gcggacgcgg
     6601 tttaagcgga tgctaatttc tgccggcgtg cggttgctga tcgggggcag aagcggcgcg
     6661 acttcgggcc ccagggcgat cagctccttc tcggcagcgt cgcgcttggc cgcctcgtcc
     6721 aaatcgagct gcctcaccag tgccgcgacc ttgctcttga gctgcgccgc cgcatcagcc
     6781 gtcgcgggcg gagcttcggc aacctgggcc agcgccaatc cagaccacag cacgagggcg
     6841 gttatcacgg cggctctcct cgcaaaagaa atcaattcct gacagattga ctgtattgta
     6901 tgccagcagc gccgggctac gccactctca agagccgatt gacccctcgt acgacgccga
     6961 ccagactaaa accatgcctc tgcgggtgac atgtccggca ggtcacaagc tgatcgttcc
     7021 cgacgatcgg gccgggaaaa ccttgcgctg cccgcggtgc agcgcggcgt tcgatgtgcc
     7081 gggcgagagg gttcagcttt cggggctgcg ggttcaggag gcgggcgtcg ggggtcggga
     7141 gtcggaagcg acgcgggttg cagtggtcgc gcagttgccg atcgtgcagg cgcaggtgga
     7201 gtcgccgccg gtggtcaagc caaagagtcg gccacgaccg ccggctcctg ccatcgtgca
     7261 agccgcgccg ccggccgtgt tggttgcgcc ggagccgaag ccgctgcagg cgatagcgct
     7321 cgctccgcct ccgccccagc cggccgtcga accagcgccc atccgtgtcc gcgaagaacc
     7381 agttgccgag gaaccagttg caaaggtcgc cccgccaccc gttgtcgaat ccccgccggt
     7441 gttgccggag gcaccagttc catcttcgcc ccagaccgag tcgagcgaga tcgcccccac
     7501 cgcgaatgca cccgccgccc gcgcgccgtc cgaagcggaa gtgcctgagc aagttgagtc
     7561 gccagctccc gcgcctcaag ctgtagttcc cgaccgccgc tggctgccgg cggcttattc
     7621 cctcgccgcc ggccttatcg cggcggcgct cttcggggca gctccggcga tctgggacgt
     7681 cgtcgattac gtacgctact acgaactcgt cgattcgccg cacgtcgccc gctgggcgct
     7741 ggtcttgctg ctcctgggcc tcgtgcaaat cgcgtatgcc ctgtttctgt tccagctgcc
     7801 cgactgggcc agcgtttggg tggtcacgct ctattcactc ggcctggcgg ggctgtatgc
     7861 gctcatgctc ggactcgtgc tgatctctcg cgaggatggc ctgctggtcg gccagcatgg
     7921 cttgcagctg gccgacaagc tcgccggcgg ccgggctgcc ctctggtgcc tggcgatgac
     7981 tagcgtcttc accatcctgg ctttcttcgc cggacgcctg agcgtgcagt ggcggcgggc
     8041 ggaatcggct cgcctggcgg cctgaattgg tcaaagccgc gaccactggt cgcggtgaat
     8101 cggaatcggc gagtgaacct gacggtagac cgcgaccaat ggtcgcggct ttgggtgcag
     8161 aatccgcagc cggcgtgaaa gattcgccca cgggccgtgt ctaatagctg gagacggttg
     8221 ctcgattcat tttcggccgc gccatgacca gcctgcccga aacccgtgcc agcttgatcc
     8281 tgcggctgcc ggatggcgcc gatgttcagg cctgggacga gtttgcggcg atctatgggc
     8341 cgctggtcta tcggctggcc cggcgtcagg gcctgcagcc ggcggacgcg gacgacctgg
     8401 tgcaggaggt gctgtcggcg gtggcccgct cggtcgaggg ctggctgcac aatggccagc
     8461 gggggccgtt ccgggcctgg ctgctgcgaa tcgccaaaaa tgcggcgatc aatgtgctca
     8521 cgcggaagcg gcatcagccg ctcgccgcag gcggccacga cctgctgctc gcccaaaccg
     8581 cagatccggc gggggagctg agtggcgaat tcgacctgga gtatcgccgc gaagtgttcc
     8641 gctgggcggc gactcagctc cgcgacctgg tcgccgattc gacctggcag gcgttctggc
     8701 ggacgagcgt cgaagagcag ccgatcgagc gcgtggccca ggagctgaag atgagcgtgg
     8761 gcagcgtcta tatcgcccgc agccgcgtga tggcccgttt gcgcgagctg gcgcggaatt
     8821 ttggggagaa cgaacgatga aagctgccgc tcaaccatgc gacgagtcgc gcctggtggt
     8881 gctgctccgc ggcaacgagg acagcgccga ttttgccgcg gcccaagggc acgtggaatc
     8941 ctgcgcggcg tgccagcagc gactctcgac gctggcggcg gaagccggcg agtgggaaga
     9001 gactcgcgag ctgcttcgcg agagcgaggg cgacgccgcc ctaaccgaag agcgcaagaa
     9061 ccggccatgg aacgtcgcgg gccgccgccc gacgaactgg accgaggcgc tggccaaaca
     9121 gctgctggct ccgccctcgc acccggaaat gctcggccgg ctcggccgct atgaaatcga
     9181 gcggctgatc ggctccggcg gcatgggcat cgtcttcaaa ggctttgaca ccgagctcaa
     9241 ccggcccgtc gcaatcaagg tgctcgcccc gcacctggct tccagcggtg cggcccggca
     9301 gcggtttgcc cgcgaggcgc gagccgcggc ggccgtcgtc cacgagcacg tcgtcgcgat
     9361 tcacaacgtc gagacgggcg gcgacgcgcc gttcctggtg atgcaatacg tcgcgggcga
     9421 atcgctgcag gcccggctcg accggcaagg gccgctggag ttgtgcgagg cactgcggat
     9481 cgcgcatcag acggccgcgg gacttgccgc ggcccacgcg caggggctgg tgcatcgcga
     9541 cgtgaagccg tcgaacatcc tgctggagca gggcctgga
//
LOCUS       DASRJB010000291         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1495089, whole genome shotgun sequence.
ACCESSION   DASRJB010000291 DASRJB010000000
VERSION     DASRJB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495089"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3436)
                     /locus_tag="VFB80_07835"
     CDS             complement(<1..3436)
                     /locus_tag="VFB80_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN33714.1"
                     /translation="MIERVIEWSIRNRFLVMFLAAGLSLLGVYAVFQTPMDATPDLSE
                     NQVIVFADWMGRSPREIEDQITYPLSRKLQGLAGVKAVRSSSEFNFSMITIIFEDGVD
                     FYFARQRVTEKLAQAGTFLPAGVIPYLAPDATALGQIFWYTVEPSPQQPIEPGRLWAI
                     NKFYIAPQLNAAAGVADVAVVGGTPLEYQIDVRPEDLRTYDVTLGEVYAAVARSNMPS
                     GGGVIQKNNAEYIIRGIGWIRDLRDIEETVIREIGGVPLLVKNVATVQLGPQFRRSVF
                     EKNGNEVTGGAVLMRHGQNPLAVTQAVKDKILELQPGLPPGVHIAAAYDRTRLIHGAI
                     HTLTEVMWHEMLIASIAILLILMHVRSVFVICVTLPLAVLFSFLLMWLLRVLGVIDIQ
                     ANIMSLAGITISIGILVDQAIVMTENATHHLKEHFGDRQVTGDTRELVIPACRTVGRP
                     IFFSVLIMLISFIPVFMLSGREGKYFHPLAFTKSFALIGVALISVTLVPALIPSFIRG
                     RLKSEEENWIVRSLIQIYKPLLTWALPRRNLVMWFFAAMLIVAAGIFPLQAIFGYGAS
                     EPAWRICFLIAFALVTALTVVFTAGWQWQALSLATLILLGLWSYHFPKIGVAFMPALD
                     EGTTLEMPITVPRASVTQAADDLKARDALLRGFPEVESVIGKAGRADTPTDPAPLDMV
                     ETFVNFRPRELWPRRVLRYPDAAWQVERVLDRLIADGFIAPPAADDRRNLVNDATQKA
                     IERFDETQRELALRRYVEFENELESKLTRFAVGETLRLIRRGMKVKLATPAAEEAVVD
                     RLAGELTPKLGPWLSRQPTLVDAVRLSEELVRLLKERSAIPPDADALQLHETGPAAVF
                     SVARDLVGVRQKTLAGELLASIERERDERWREHVRHVNWELFDHGVESFTWYALEETV
                     RAAQSASLLAKAARGAEAQRFAAAALAVQLGRQGNAADVAPFQSLRETLEAPMRKRVF
                     FWPRSHGTKGDLVDDEMSRVLQVPGWSNIFTQPIINRIEMLSTGVRTDIGVKVFGSDL
                     ETIDRVGKEIEAVLRPIRGAKDVVAAPIMGKGYIDIQIDRAAAARYGISVEDVQTEIE
                     MALAGRAVTFTVEKRDRFPVRVRYARSSREDEESVRRLLISPQGMSAVTTTSMAPPH"
     gene            complement(3433..>3677)
                     /locus_tag="VFB80_07840"
     CDS             complement(3433..>3677)
                     /locus_tag="VFB80_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33715.1"
                     /translation="KLVPADRLLAEQQRTCPILPQSRLGSMGPPLKLTLAGETVFICC
                     SGCQDQATKTAGETAQRARELRRSQPPAAKNAGDRR"
BASE COUNT          623 a         1188 c         1243 g          623 t
ORIGIN      
        1 catgcggcgg ggccatgctg gtcgtggtga cggcgctcat cccctgcggg ctgatcagca
       61 ggcggcggac gctctcttcg tcctcgcggc tgctgcgggc gtagcgcacc cggacgggaa
      121 accggtcgcg tttttcgacc gtgaacgtga ccgcgcggcc cgccagggcc atctcgattt
      181 cggtctgcac gtcctcgacc gagatgccgt agcgggcggc ggcggcgcgg tcgatctgga
      241 tgtcgatgta gcccttgccc atgatcgggg cggcgaccac gtccttggct ccacggatcg
      301 gccgcaggac cgcctcgatc tccttgccga cgcggtcgat cgtctccagg tcggagccaa
      361 agactttcac gccgatgtcg gtgcggacgc cggtggagag catctcgatc cggttgatga
      421 tcggctgcgt gaagatgttg ctccagccgg gcacttgcag cacgcggctc atttcgtcat
      481 cgaccaggtc gcccttcgtg ccgtggctgc ggggccagaa gaagactcgt ttccgcatcg
      541 gcgcttcgag cgtttcgcgc aggctctgga agggcgcgac atccgcggcg tttccttgcc
      601 ggcccagctg caccgccagc gccgcggcgg cgaagcgctg cgcttccgcg ccgcgagccg
      661 ctttggccag cagcgaggca ctctgagccg cgcggacagt ctcttccagg gcgtaccagg
      721 tgaacgactc gacgccgtgg tcgaacagct cccagttcac gtgcctgacg tgctcgcgcc
      781 accgctcgtc gcgctcgcgc tcgatgctgg ccagcaattc cccggcgagt gtcttttggc
      841 ggacgcccac caggtcgcgg gcaacggaaa aaacggccgc cgggccggtt tcatggagct
      901 gcagcgcgtc ggcatcgggc gggatggcgc tgcgctcttt gaggagccgc accagctctt
      961 cgctcaggcg gacggcgtcc accagcgtcg gctggcggga gagccaagga cccaacttcg
     1021 gcgtcaactc gccggcgagc cggtccacca cggcttcctc cgcggccggc gtggcgagct
     1081 tgaccttcat gccgcggcgg atcaatcgca gcgtttcgcc aacggcaaac cgcgtcagct
     1141 tggattccag ctcgttctcg aactcgacgt accgccgcag ggccagctcg cgctgggtct
     1201 cgtcaaaccg ttcgatggcc ttctgcgtgg catcgttgac caggttgcgg cggtcgtccg
     1261 cggctggcgg ggcaatgaag ccgtccgcaa taagccgatc gagcacgcgc tcaacctgcc
     1321 aggcagcgtc tggatagcgg aggacgcggc ggggccagag ctcccggggc cggaagttga
     1381 caaaggtctc gaccatgtcg agcggggcgg gatcggtggg agtatcggcc cgcccggcct
     1441 tgccgatgac cgattcgacc tcgggaaagc cgcgaagcag cgcgtcgcgg gccttgaggt
     1501 cgtcggcggc ctgcgtgacg ctggcccgcg gcacggtgat cggcatctcg agcgtggttc
     1561 cctcgtcgag ggccggcatg aaggccacgc cgatcttggg aaagtggtac gaccagaggc
     1621 cgagcagaat tagcgtggcc agcgatagag cctgccactg ccagccggcc gtgaagacga
     1681 ccgtcagcgc cgtgacgagg gcgaacgcga tcaggaagca gatccgccag gccggctcgg
     1741 acgcgccgta gccaaagatc gcctgcagcg gaaagattcc cgccgccacg atcagcatcg
     1801 ccgcgaaaaa ccacatcacc agattccgtc gcggcaaggc ccacgtgagc agcggcttgt
     1861 agatctggat caagctgcga acgatccagt tttcttcctc gctcttcagg cggccgcgga
     1921 tgaacgaggg aatcagggcc ggcaccagcg tcaccgagat cagggccacg ccgatcaggg
     1981 cgaaactctt ggtgaacgcc agcgggtgaa agtacttgcc ttcgcggccg ctgagcatga
     2041 agaccgggat gaacgaaatc agcatgatca gcacgctgaa gaagatcggc cggccgaccg
     2101 tgcggcaggc cgggatgacc agctcgcgcg tgtcgcccgt gacctggcgg tcgccaaaat
     2161 gctccttcag gtggtgcgtg gcgttctcgg tcatgacgat cgcctgatcg acgaggatcc
     2221 cgatcgagat cgtgatgccg gccagcgaca tgatgttggc ctggatgtcg atgacgccca
     2281 gcacgcgaag cagccacatc agcaggaacg agaacagcac ggccagcggc agcgtgacgc
     2341 agatcacaaa cacgctgcga acgtgcatca ggatcagcag gatcgcgatc gaggcgatca
     2401 gcatttcgtg ccacatcacc tcggtcagcg tgtgaatcgc gccgtggatc agccgcgtgc
     2461 ggtcgtaggc ggcggcgatg tgcacgcccg gcggcagtcc gggctgcagc tcgaggattt
     2521 tgtctttcac ggcctgcgtg acggcgagcg ggttctggcc gtgccgcatc aggacggccc
     2581 cgccggtgac ttcgttgccg ttcttttcaa agacgctgcg gcggaactgc gggccgagct
     2641 gcaccgtggc gacgtttttc accaagagcg gcacgccgcc gatttcccgg atcacggtct
     2701 cttcgatgtc gcgcaggtcg cgaatccagc cgatgccgcg gatgatgtac tcggcgttgt
     2761 tcttctggat gactccgccg ccggagggca tgttgctgcg ggcgacggcc gcgtacacct
     2821 cacccagcgt cacgtcatac gtccgcaggt cctccggccg gacgtcgatc tggtattcca
     2881 gcggggtgcc gccaaccacc gccacgtcgg cgacgccggc ggctgcgttc agctgcggcg
     2941 cgatgtagaa cttgttgatc gcccacagtc ggccgggctc gatcggctgc tgcggactgg
     3001 gctcgacggt gtaccagaag atctggccca gcgcggtggc atcgggcgcg aggtagggaa
     3061 tcacgcccgc cggcaggaac gtaccggcct gggcgagctt ctcggtcacg cgctggcggg
     3121 cgaaatagaa atcgacgccg tcctcgaaga tgatcgtgat catcgagaag ttgaattccg
     3181 acgacgagcg gactgctttc acgccggcca ggccctgcag cttgcgcgac agcgggtagg
     3241 tgatctggtc ctcgatttcg cgcgggctgc ggcccatcca atcggcgaac acgatcacct
     3301 ggttctcgct caggtcaggc gtcgcatcca tcggcgtctg aaagaccgca tagacgccca
     3361 gcagcgacaa gccagccgcc aggaacatga cgagaaaccg gttgcggatc gaccattcaa
     3421 tgacgcgttc gatcatcggc ggtctcccgc gtttttggcg gccggaggct gcgatcgccg
     3481 cagctctctc gcgcgctgag ccgtctcgcc ggcggtcttc gtcgcctgat cctggcagcc
     3541 gctgcagcag ataaagacgg tttcgcccgc gagagtcaac ttcagcggcg gacccatcga
     3601 gccgagccgg ctctggggca agatcgggca ggtccgttgc tgctcggcta gtagccgatc
     3661 ggccggcact agtttgg
//
LOCUS       DASRJB010000292         5152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_83850, whole genome shotgun sequence.
ACCESSION   DASRJB010000292 DASRJB010000000
VERSION     DASRJB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83850"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3744)
                     /locus_tag="VFB80_07845"
     CDS             complement(<1..3744)
                     /locus_tag="VFB80_07845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33716.1"
                     /translation="MKAEDIFLAALERKSPAERLAFLDGACGDDPFLRAQVVGLLHSH
                     EEAGSFLDAPLFDSPPTIDQTGPERLGTQIGPYKLLQKIGEGGFGVVYMAEQQEPVRR
                     RVALKVIKPGMDTRQVIARFEAERQALAMMDHANIARVMDAGATASGRPYFVMELVRG
                     VPITAYCDQNHLPVRERLELFAAVCHAIQHAHQKGIIHRDIKPSNVLVTSQDGQAVVK
                     VIDFGVAKAMGQQLTDKTLFTDFAQMIGTPLYMSPEQAELSSADIDTRSDIFSLGVML
                     YELLTGSTPVDKEQMKQAAFDEIRRLIREEEPQKPSTRISSAEAAPSIAAQRHTEPAK
                     LARLVRGELDWIVMKALEKDRNRRYETASAFAKDLEHFLHDEPVLACPPSAVYRLRKF
                     ARRNKALLATVATIAAALVIGIAVSTWQAIRATKAENLAQSRLVSANQATARATAAGK
                     EAFRQKKQAETNAAETEVQRKEAEAQRNVAEKNFVKARTSVDDYFVKVSESQLLKGPG
                     LQPLRRELLEAALKFYEELAQQRSKDPALQKELAGAHYRVGRVRTELGAREAALQAYA
                     KAQELYEGLLKAEPGNIDLEHGRARCQFWRGQSSEAIAAWEKLIERDRSRTDIRRELA
                     SAYNSIANSEKNAGKPAESLQWHKKAHEIRKELAEATPDNIDDRYALGQTLNNIAVLL
                     SGNDADALALYRRALEHGQFAYNRAPQNVNYGRLVGTAQGNIGICESRLGSKEPALKS
                     FQLAIDVWKKLALDNPAVPDLHANLLANYRRLADYQRDLGQSEKWTGTIRLAQQVLER
                     FPQQSASDLYSLACLHAVTAALSAKDPLTATAAELVESQAVVDRESNEAVDALDRAIR
                     AGFSDLVLLQIDKDLDPLRQREDFKMVLAKLDETLKSKLVAQAEQERGARYDALAKAA
                     AQGATPEALAASKEKLAIDKGLAAEDPQRLDRQADVAATQHTIGLIQLSLEQLEEAGQ
                     SLAQAEATRQFLVTKEPSKLSYQADLAATRLALGTLDWKTNRLADGAARWQQGLTQLE
                     EVVHAEPENNRFVSQLAAAHAAVGNSYGEIGLWKEAAGHYAKAFAAQPSGNSYAWLTV
                     GYLSFYVGDRESYDRVCREALERSKGNIDRRLLSDLTACINLSPKPLIESAKLVEMAQ
                     QVLAVEPTAGWRRFRVSLAQYRAEQFAEALHTLEGYSGFSETWPLKAMIYHRLGQPEQ
                     ARPELAKIDRHFDKTFGNALAAESLKLPGWNGLVMVQVLRREAH"
     gene            complement(3824..4468)
                     /locus_tag="VFB80_07850"
     CDS             complement(3824..4468)
                     /locus_tag="VFB80_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33717.1"
                     /translation="MSEVTRLLSQIESGDPKAAERLLPLVYDELRKVAAARLAHENPG
                     QTLQATALVHEAYLRLVGPGAGGRESGVEDGELPSTPDSRTPTPLFDSRGHFFAAAAE
                     AMRRILVENARRKKRIKHGGEFERVDVELAELPTRMEADELIALDEALARLREQDPPK
                     AQLITLRYFGGLTIEQAAEVLKISRVTAHRYWTFARAWLHQEITGEARRPPKSA"
     gene            complement(4522..>5152)
                     /locus_tag="VFB80_07855"
     CDS             complement(4522..>5152)
                     /locus_tag="VFB80_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33718.1"
                     /translation="ARGQRSAMTAAEKRGAVLFFGQANCVACHRASGFANEMFSDFAN
                     HRLGVPQLAPRFGLNTGNVHFDGPHADEDFGAEQISGQKSDRYKFRTSPLRNIALAPT
                     FFHNGCFTRLEDAIRHHLDVRRSLAEYDPAAAGVAADLRLSEMPRAALLDDLDPLVDE
                     PLELSRREFDDLVEFVRTGLLDERATPERLLKLVPRELPSGLPLHEFEE"
BASE COUNT          762 a         1655 c         1724 g         1011 t
ORIGIN      
        1 gtgcgcctcg cggcggagca cctggaccat gaccaggccg ttccagcccg ggagcttcag
       61 cgattcggct gccaaggcat tcccgaacgt tttgtcgaaa tggcggtcga tcttggcaag
      121 ctccggacgc gcctgctcgg gctgtcccaa acggtggtag atcatggcct tcaagggcca
      181 ggtttcggag aatcctgaat agccttcgag agtgtgcagg gcctcggcga actgctccgc
      241 gcgatactgg gccagcgaca cgcggaaccg ccgccagccg gcggtcggct ccaccgccag
      301 cacctgctgg gccatctcaa ccagcttcgc gctttcgatc agcggcttgg gagataagtt
      361 gatgcaagcc gtcaggtcgg agagcagtcg gcggtcgata tttcctttgg accgttctag
      421 ggcctcgcgg caaacccggt catagctctc ccggtctccc acgtagaagc tcaggtatcc
      481 cacggtgagc caggcgtagc tgttgcccga gggctgtgcg gcgaaagcct tggcatagtg
      541 gccggctgct tctttccaga gaccgatttc gccataggaa ttcccgacgg cggcgtgggc
      601 cgcggccaac tggctgacaa agcgattgtt ttctggctcg gcgtggacga cttcttcgag
      661 ctgcgtcagg ccctgctgcc agcgggcagc gccatcggcc aggcggtttg tcttccagtc
      721 gagggtgccc aaagccagac gggtggcggc gagatccgcc tggtaggaga gcttactcgg
      781 ctccttggtc acgaggaact ggcgagtcgc ttcggcctgg gcgagcgact gccccgcttc
      841 ctcgagttgt tcgagcgaaa gctggatcaa gccgatggtg tgttgggtgg cggctacatc
      901 ggcctggcga tcgagccgct gggggtcttc tgcggccagg cccttgtcga tggctaattt
      961 ctccttgcta gccgcgagag cctcgggcgt ggcgccttga gcggccgcct tggccagcgc
     1021 gtcgtaccgg gctcctcgct cctgctcggc ctgggcaacc agtttcgact tcagtgtctc
     1081 gtcgagcttt gccaatacca tcttgaagtc ttcgcgctgg cgtagcggat cgaggtcttt
     1141 gtcgatttgg agcaagacca ggtcgctgaa tcccgcccgg atggcccggt cgagcgcatc
     1201 caccgcctcg ttggactcgc ggtccacgac cgcttgcgac tccacaagct ccgccgccgt
     1261 ggccgtgagc gggtccttgg ccgagagggc ggccgtcacc gcgtgcaggc aagccaggct
     1321 gtacaggtcg ctagccgact gctgtgggaa ccgctccaac acctgctgag ccagccgtat
     1381 tgtgccggtc cacttttcgc tctgtccaag atcccgctgg tagtcggcca gccggcggta
     1441 gttggcgagg agattcgcgt gcaggtcggg gaccgccggg ttgtccagcg ccagcttctt
     1501 ccagacgtcg atcgcgagct gaaaggattt cagcgcaggc tccttgctac ccagccggct
     1561 ctcgcagatg ccgatgttgc cctgggcggt gcccaccagg cgcccatagt ttacgttctg
     1621 cggcgcacgg ttataggcga actggccgtg ctccagcgcg cggcgataga gggccagggc
     1681 atcggcgtcg ttgccggaca gtaggacagc gatgttgttg agcgtctgac cgagggcgta
     1741 gcggtcgtca atgttgtcgg gcgttgcctc ggcaagctcc ttgcggatct cgtgcgcctt
     1801 tttatgccac tgcagagact cggccggttt gccagcgttc ttctctgaat tggcgatgct
     1861 gttgtaggcg ctcgccagct cgcggcggat gtcggtgcgc gagcggtccc gctcgatcag
     1921 tttctcccag gcggcgatcg cttcggagga ttgtccccgc cagaactggc agcgtgcccg
     1981 gccatgctcc aggtcgatgt tccccggctc ggctttgagc aggccttcgt agagctcctg
     2041 ggccttggcg tacgcctgaa gtgccgcctc gcgggccccc agctcggtgc ggacccgccc
     2101 cacgcgatag tgcgcgcccg ccagctcttt ctgaagggcc gggtctttgc tccgctgctg
     2161 tgccagctct tcgtagaact ttagcgccgc ttcgagcagc tcgcggcgca gcggctgcaa
     2221 gcccggtccc ttcagcagtt gactctcgct gaccttcacg aaatagtcgt caacggaggt
     2281 gcgggccttg acgaaattct tctccgccac gttgcgctgg gcttcggctt ccttgcgctg
     2341 gacttcggtt tcggcggcgt ttgtctcggc ctgtttcttc tgccgaaatg cctccttgcc
     2401 ggcggcggtg gcccgcgctg tggcctgatt cgcgcttacc aggcggctct gggcaaggtt
     2461 ctcggccttg gtggcgcgga tcgcctgcca ggtgctgacg gcgatgccga tgactagcgc
     2521 ggcggcaatc gtggccaccg tagccagcag agctttgttt cgccgggcga acttgcgcag
     2581 tcgatagacc gccgacggcg ggcaggccag aaccggctcg tcgtgcagaa agtgctccag
     2641 gtccttggcg aaggcgctgg ccgtttcgta gcggcgattg cggtcctttt cgagggcctt
     2701 catcacgatc cagtcgagct cgccgcggac caggcgggcc agcttggccg gctcggtgtg
     2761 ccgctgggcg gcgatgctgg gcgcggcctc ggccgagctg atccgcgtgc tgggcttctg
     2821 cggctcttcc tcgcggatca ggcggcgaat ctcgtcgaag gccgcctgct tcatctgctc
     2881 cttatcgacc ggcgtcgagc ccgtgagtag ctcgtagagc atcacgccga gcgaaaagat
     2941 gtcgctccgc gtgtcgatgt cggcgctgct cagctccgcc tgctcggggc tcatgtagag
     3001 cggcgtgccg atcatctggg cgaagtcggt gaacagcgtc ttgtcggtga gctgctgccc
     3061 catcgccttg gccacgccaa agtcgataac cttcactacg gcctggccgt cctgcgacgt
     3121 gaccagcacg ttgctgggct tgatatcgcg gtggatgatg cccttttgat gcgcgtgttg
     3181 gatcgcgtgg cagaccgccg cgaacagctc gagacgctcg cgcaccggca ggtggttctg
     3241 gtcgcaatag gccgtgatgg gcacgccacg gaccagttcc atgacgaagt aaggccgccc
     3301 agaggcggtt gcaccggcgt ccatcacgcg ggcgatattg gcgtggtcca tcatcgccag
     3361 ggcttgccgc tcggcctcga agcgggcgat tacttgccgg gtgtccattc cgggcttgat
     3421 gaccttcagg gccacgcggc ggcggaccgg ctcctgctgc tcggccatgt agacgacccc
     3481 gaagccgcct tcgccaatct tctgcagcag cttgtaaggg ccgatttgtg tgccgagtcg
     3541 ctccggcccg gtctgatcga tcgtcggcgg cgaatcgaac agcggcgcgt cgagaaagct
     3601 gccggcctct tcatgggagt gcaacaatcc caccacttgg gcccgcagga agggatcgtc
     3661 gccgcaagcg ccgtcgagaa acgccaggcg ctcggcgggc gactttcttt ccagtgcagc
     3721 cagaaaaatg tcttcggcct tcatggaacg ccagggcgat gtgccttgct ccactatcca
     3781 atgcgacgcg ggcgggcggc gcgctccagg accgcggcgg ttttcaggcc gattttggcg
     3841 gcctgcgcgc ttcgccggtg atttcctggt gcagccaggc ccgggcaaac gtccagtagc
     3901 gatgcgccgt aactcgcgaa atcttcagca cttccgcggc ctgctcgatc gtcaggccgc
     3961 cgaaatagcg aagcgtaatc agctgggctt tgggcggatc ctgttcgcgc aggcgggcca
     4021 gcgcttcgtc gagggcaatc agctcgtcgg cctccatccg cgtcggcagt tcggccaatt
     4081 ccacgtccac gcgctcgaac tcgccgccgt gcttgatccg cttcttgcgg cgggcgttct
     4141 cgaccagaat ccgccgcatg gcttccgccg cagccgcgaa gaaatggccg cgggaatcaa
     4201 agaggggagt cggggtccgg gagtcggggg tcgaaggaag ctcgccgtct tcgactcccg
     4261 actcccgacc cccggctccc ggtcccacca gcctcaaata cgcctcgtga accagcgccg
     4321 tggcctgcag cgtttggccc gggttctcgt gcgccagcct ggcagcggcc acctttcgca
     4381 gttcgtcata gaccagcggc agcagccgct cggcggcttt gggatcgccg gattcgatct
     4441 gcgagaggag gcgtgtgact tccgacatgg ctgccattgt gctccctgtc cccttggaat
     4501 gggagaggga agcggccgcc gtcactcctc gaactcgtgc aggggcagcc cgctgggcag
     4561 ctcgcggggg acgagtttca gcagccgctc gggcgtggcc cgttcgtcta acaggccggt
     4621 ccgcacgaac tcgaccaggt cgtcgaactc gcggcggctg agctccagcg gttcgtccac
     4681 gagcggatcg aggtcgtcca gcagcgccgc ccgcggcatc tcgctcagcc gcaggtcggc
     4741 cgcgacgccg gcggctgcgg ggtcgtactc cgccagcgag cgccttacgt cgaggtgatg
     4801 ccggatggcg tcttccagcc tcgtgaagca gccgttgtgg aaaaaggtcg gggccagggc
     4861 aatgttccgc agcggcgaag tgcggaactt gtagcggtcg ctcttctggc cactgatctg
     4921 ctcggcgcca aaatcctcgt cggcgtgcgg gccgtcgaag tggacgttgc cggtgttcag
     4981 tccgaaccgc ggcgcgagct gcggcactcc caggcggtgg ttggcaaaat cgctgaacat
     5041 ctcgttggca aagccgctgg cgcggtggca ggcgacgcag ttggcctggc cgaagaacag
     5101 cacggccccg cgcttctcgg ccgcggtcat cgcgctgcgc tggccgcggg ca
//
LOCUS       DASRJB010000293         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2622376, whole genome shotgun sequence.
ACCESSION   DASRJB010000293 DASRJB010000000
VERSION     DASRJB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622376"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1084
                     /locus_tag="VFB80_07860"
     CDS             302..1084
                     /locus_tag="VFB80_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN33719.1"
                     /translation="MLARKTIFPNVIEINFQAGEVLGCCVYLIFDGGEWVLIDIGLEE
                     STEEIIELIRQMDFPLSKCKMLIATHADVDHIQGLAKAKHLLKAPVYAHPKAVAALES
                     GDKLHTFARIEAQNLDMPMPVVKIDGTLDEGDKIQVGGLTIDVWLTPGHTDSQLSFRL
                     GDLLFSGDNIYRDGCVGAIDAHHGSDISAFIQSLRRIRGSDVKWLLPSHGPFFRKDNA
                     LLDRTIARLETYLHMADFGTCAIDWPLMDQWEQELAEGKRPA"
     gene            1265..1762
                     /locus_tag="VFB80_07865"
     CDS             1265..1762
                     /locus_tag="VFB80_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33720.1"
                     /translation="MAEAGESWRGWLEEHGRALVLLARQWVPTHADAEDVVQEAFVRC
                     WRSRDRAREPVPYLFASVRRCALDWQRSRQRQARREQAAARPEAEPLLASGVEQDERR
                     QAIEAALRELPIEQAEVLVMRIWGGLSVPQIAAALEAPVNTVASRYRYALARLREQLA
                     EESIR"
     gene            1759..2214
                     /locus_tag="VFB80_07870"
     CDS             1759..2214
                     /locus_tag="VFB80_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33721.1"
                     /translation="MNDEIDSLEAELAALAPRDLSPQLAGRIAAELEQEGPAPLRRWR
                     LGQVLAAIAVGAVAASAVAAAWLWRNDQRTAQADSPAAPIALPVATAFDPALPSVWTF
                     RRAADEPAGQLDGLLDRHSGRTRLEQPHYVQIRGFGASDVERNDLLGEL"
     gene            2214..3356
                     /locus_tag="VFB80_07875"
     CDS             2214..3356
                     /locus_tag="VFB80_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33722.1"
                     /translation="MRSFFFVCLLLVGLACPALSAAQNPFQRTPLAENAALQYWQAFA
                     QMQSLDKDQMKVLEEWNTVSLDDPAVKKVLDAGQSSLMYLHRGARMKQCDWGLDYGDG
                     ISMLMPHLAKARDLARLAALHGRQEFERGSPKLARGDATSIMVLARHVGRDPTMICVL
                     VRFGIEGLAVDLFAPHVTELKAPYSQAVAMFEALPPAPGVLQTLVVEKNYFAAWIAKK
                     LREEEARQPGAGLTLWKNFVLGADVPEAIKQLKIEEAIRRTEELLPVYDELAKLVALP
                     KDQFDAQYPAFIQKAKEANPVAGLIVPAVDQLLAKERRHQARLAMSMAATAVAEGGPD
                     KLKDIKDPFGDGPFTYRQLDKGFELQSKLLFEGQPVTLKAGQGAKK"
     gene            complement(3793..>4283)
                     /locus_tag="VFB80_07880"
     CDS             complement(3793..>4283)
                     /locus_tag="VFB80_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33723.1"
                     /translation="CEREIHPDTPLARFLPSERRREFWTTAQRQFGLRLPALRLPLSM
                     QIAGHWLTLAAVGRTVLIGLVLGAKWIVLPLVFVTWLLTSAFYDWITRPWATELPGLE
                     TFGDLSRMLLARNVKTFRSRFGLKPNRDEIYAAVAAILAEQLGVDPREITWDTRFDEL
                     IG"
BASE COUNT          788 a         1381 c         1393 g          721 t
ORIGIN      
        1 gtcttcgggc gcggcggtga gaacgagcag cagcacggcg ggcagtgaca taggacgctc
       61 ctttttgaca accgcatcag tttggcgtgg cggcgaacaa atcgccagca cttgattaac
      121 tcacaagcaa cgattcgtag ctttcgctga gacagactct caataaccct catttccgcc
      181 ggaatttgca gccggctgcc ggcacggccg ttgctggctt tgagcggcgt tcctacaatc
      241 ccgcaggcgg ctcgcaggat catgttgccg ccgagacctt tgcctcgttg cccgacctcc
      301 catgctggcc cgcaagacga tcttcccgaa cgtgatcgag atcaacttcc aggcggggga
      361 agttctgggc tgctgcgtct atttgatctt cgacggcggc gagtgggtgc tgatcgacat
      421 cggcctggag gaatcgaccg aggagatcat cgagctcatc cggcagatgg atttcccgct
      481 cagcaaatgc aagatgctga tcgccacgca cgccgacgtc gatcacatcc agggcctggc
      541 caaggccaag cacctgctca aggcgccggt ctatgcccac ccgaaggccg tggcggcgct
      601 cgagtcgggc gacaagctcc acaccttcgc ccgcatcgag gcccagaacc tcgacatgcc
      661 gatgccggtc gtcaagatcg acggcaccct cgacgagggc gacaaaatcc aggtcggcgg
      721 gctgacgatc gacgtctggc tgacgcctgg ccacaccgac agccagctca gcttccgcct
      781 cggcgacctg ctcttctccg gcgacaacat ctatcgcgac ggctgcgtcg gtgcgatcga
      841 cgcccaccac ggcagcgata tctcggcctt catccaatcg ctgcggcgga ttcgcggcag
      901 cgacgtgaag tggctcctcc ccagccacgg ccccttcttc cgcaaggaca acgcactgct
      961 cgatcgcacc atcgcccgcc tcgagaccta tctccacatg gccgacttcg gcacctgcgc
     1021 catcgactgg cccctcatgg accagtggga gcaagagctc gccgaaggca agcggccggc
     1081 gtagccgcga ccctgtcctt agccgggact ggacccgaag tccgcatggt gcggtgtctc
     1141 ccaaatctcc gggagcaggt ttgctccggg ctaagagctt tttcgatcga atagacggct
     1201 tgcgaccccc gcggcgtcta tcttgtagag aacaccaagg cagcagccgc gagcgggagc
     1261 ggttatggcg gaagcggggg aatcctggcg aggttggctc gaggagcacg ggcgggcgct
     1321 cgtactgctg gcccggcagt gggttccgac gcatgccgat gcggaggacg tggttcagga
     1381 agccttcgtc cgctgctggc gctcgcgtga tcgggcccgc gagcctgtgc cctacctgtt
     1441 tgccagcgtc cggcggtgtg cgctcgattg gcaacgcagc cggcagcggc aggcgcggcg
     1501 cgagcaggcg gcggcccggc cggaggcgga gccgcttttg gccagcggtg tggagcagga
     1561 cgaacgccgg caggcgatcg aggccgcgct ccgcgaattg ccgattgagc aggcggaagt
     1621 gctggtcatg cgaatctggg gcggcctgag cgttccgcag atcgcggcgg cgctcgaagc
     1681 tccggtcaac acggtggcct cgcgctaccg ctacgccctg gcccgcctgc gcgagcagct
     1741 ggccgaggag agcatccgat gaacgacgaa attgactccc tggaagccga actggcggcg
     1801 cttgcgccgc gcgacctttc gccgcagctg gcaggccgga tcgccgccga attggagcag
     1861 gaggggccag cgcccttacg gcggtggcgt ttgggccagg tgctggccgc gatcgccgtc
     1921 ggcgccgtgg cggccagcgc cgtcgccgcc gcgtggctgt ggcgaaacga ccagcgaacc
     1981 gcgcaggccg attcgcccgc tgctcccatc gcgctgcctg tggcgactgc cttcgatccg
     2041 gcgctgccca gcgtctggac ctttcgccgg gccgccgacg agccggcggg ccagctcgac
     2101 ggcctgctcg accggcactc cggccgcacg cggctggagc agccgcacta cgtacaaatc
     2161 cgcgggtttg gtgcgtcgga cgttgaacga aacgacttac ttggagaatt gtgatgcgca
     2221 gcttcttctt cgtttgtctc ctattagttg gattggcctg tcccgcgctt tccgcggccc
     2281 agaacccgtt tcaacgcaca ccgctggcgg aaaacgcggc gctgcaatac tggcaggcct
     2341 ttgcccagat gcaatcgctg gacaaggacc agatgaaggt cctggaagaa tggaacacgg
     2401 tttcgctgga cgatccggcg gtgaagaagg tgttggacgc cggtcagagc agcctgatgt
     2461 acttgcatcg cggggctcgt atgaagcagt gcgactgggg cctggattac ggtgacggca
     2521 tcagcatgct catgccgcac ctggccaagg ctcgcgacct ggcccgcctg gccgcgctgc
     2581 acgggcggca ggaatttgag cgcggcagtc cgaaactggc ccgcggcgac gccacctcca
     2641 tcatggtgct ggcccgccac gtcggacgcg atccgaccat gatctgcgtc ctggtgcggt
     2701 tcggcatcga ggggctggcc gtcgatctgt tcgcgcccca cgtcaccgaa ctcaaggccc
     2761 cgtactctca ggcggtggcc atgttcgagg cgctaccgcc ggcgcccggc gtgctccaga
     2821 cgctcgtcgt cgagaagaac tattttgccg cttggatcgc caaaaagctc cgcgaggaag
     2881 aagcccgcca gcccggcgcg gggctgacgt tgtggaagaa cttcgtgcta ggcgcggacg
     2941 ttccggaggc gatcaagcag ctcaagatcg aagaggccat ccggcgcacg gaagagctgt
     3001 tgcccgtcta tgacgagctg gcgaagctcg tcgcgctgcc caaggatcag ttcgacgctc
     3061 agtatcccgc gttcattcag aaggcgaagg aagctaaccc ggtggcgggc ctgatcgtgc
     3121 ccgccgtgga ccaactcctg gccaaagaac gccgccacca ggcgcggctg gcgatgagca
     3181 tggcggcgac cgccgttgcc gagggcggcc ccgacaagct caaggacatc aaagacccct
     3241 tcggcgacgg tcccttcacg tatcgccaac tcgacaaggg atttgagcta cagtcgaagc
     3301 tgctcttcga gggccagccg gtcacgctca aggcgggcca gggcgcgaaa aagtagcctc
     3361 ggcgttcagc cgaggatcag aaggctactt gttcccaggt tccctgggaa cacaattccg
     3421 cgtggctcca gcctcgctgg tccgaatgcc ctaaacgatc attcgccgaa ggtaaggata
     3481 attcccatgc ggggcggatt tttgcgctcc tcatgtcatc ggcgaacgcg ccgcaactgc
     3541 ttgcgggtca atggcttccg acgaaccgcc cggcggaatc gcggatgacg aacagaagag
     3601 ttagagaacg cgaagcggaa ttatccttac cctcgcggtc catcaccagg ggcggcgatt
     3661 ctgccccaga ttgcaacgac caaattgcca gtgagcgggc gatcttcgcg aggccgccaa
     3721 attggcagcc tgcctcgcca acttgaacta cactgcaaat agcgtaccaa tgttcagaag
     3781 tgccgccgca catcagccga tcaattcgtc gaaacgcgtg tcccaggtga tctcgcgcgg
     3841 gtcgacgccc agttgttccg ccaggatggc ggcaacggcg gcgtagattt cgtcgcggtt
     3901 gggcttgagg ccgaaccgcg agcgaaaggt tttgacgttg cgggccagca gcatgcgcga
     3961 caggtcgccg aacgtttcca ggccgggcag ctcggttgcc cagggcctcg tgatccagtc
     4021 ataaaaagcc gaggtcaaaa gccaggttac aaacaccagc gggagcacga tccatttggc
     4081 ccccagcacg aggccgatca ggacggtgcg ccccaccgcg gccagcgtca accagtggcc
     4141 ggcgatttgc atggagagcg gtagccgcag cgccggcagc cgcagaccga attggcgctg
     4201 ggcagtggtc cagaactccc gccgccgctc gctgggcagg aaccgcgcga gcggggtgtc
     4261 gggatggatt tcgcgttcgc aga
//
LOCUS       DASRJB010000294        15948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4032961, whole genome shotgun sequence.
ACCESSION   DASRJB010000294 DASRJB010000000
VERSION     DASRJB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032961"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFB80_07885"
     CDS             <1..1118
                     /locus_tag="VFB80_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN33724.1"
                     /translation="GWAQAQVAAARGNWVQVGLRNNPAVGYLGQQLGSGGLAEQHAFM
                     IDQEIITAGKRQLSRAEAEQRVAIAEQQLAAQQQRVLTDVRLAFYDALLAQQRLKLAQ
                     ELLAIAQLGQAAADRLHRAGETSRVDLIQSNLEVESAEIELNKATNQHTYVWMGLSAI
                     IGLPHLPPAVLRGDLDAVPAELTWDQTLARLLTASPELAAAASEIERARWGLARAQRQ
                     VIPNVRFQGAVMQDNGIGGKTDGIVQLLVPLPIFNRNQGAIQQARAEVVAAERAIQQL
                     ELDLQYRLAPVYQRYATAASQVRRYRERILPKAQESLGLVRRGYEAGEFPYLLLLNSQ
                     RTYFQTNLQYLESLRELWASSVEIDGLLLRDSLSARP"
     gene            1176..2342
                     /locus_tag="VFB80_07890"
     CDS             1176..2342
                     /locus_tag="VFB80_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN33725.1"
                     /translation="MPAESTTQILARRYDTLRPVALEIAGGRIAKISPAAGGAELPIV
                     APGLVDLQINGYGGIEFNDPQLTIDKVRQVARSQDPFGVTAFLATLTTDSHAVLAHGL
                     ATIAAAIGELPEVATRIPGIHLEGPFICPDDGPRGAHPKQHVRPPDWDEFRRLQDAAA
                     GRIKLLTISAEYASSPDVIRRVAESGVLVAIGHTQASGEQIQAAVDAGARMSTHLGNG
                     SHPMIRRHPNYIWDQLAEDRLVASLIVDGHHLPPAVVKSMVRAKTPPHVVLVSDITSM
                     GGMPPGKYQTGLGELEVLPSGKLVPAGRPGILAGASLPIHVCVANVMRFAGVDLRTAI
                     DMASVAPARLIGLDHPGLEVGAPANLFLFDLPASEAAPLQVHSTWHEGRRSHPH"
     gene            2354..2794
                     /locus_tag="VFB80_07895"
     CDS             2354..2794
                     /locus_tag="VFB80_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm17 family immunity protein"
                     /protein_id="HZN33726.1"
                     /translation="MPIQDYFVGGAAALLGGFLLLGAALDAAWLMSLKRPRLLAESIG
                     KAAARWTLGLVGVGLIALGALIASGWRVDWSWLGQRTVSSAPSLRCSNPLHRTIGCGG
                     YLANCGSLCDFWQSRNREERPETISRIHAQLRHSERLAKSPWFI"
     gene            complement(2791..4104)
                     /locus_tag="VFB80_07900"
     CDS             complement(2791..4104)
                     /locus_tag="VFB80_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33727.1"
                     /translation="MSRLSRRRFLQTTAAASAVFAAPYFIPQRAFGANERVVLGFVGV
                     KNRGMQNMEGFGIVGRGAANRRADCAAVCDVDKNVFASAVKRVEEAGQKPSVFGDYRQ
                     LLERKDIDAVVLSVPDHWHALMTVDACRAGKDVYCEKPLTLFVTEGRKMVEVARETGR
                     VVQTGSQQRSDDRFRLACELARNGKLGKLQTVLVGIPAPNHMSPVDKKDTTAAPDSDP
                     PPELNYDLWLGPAPQRPYNVNRVHYKFRFFWDYSGGQMTNFGAHHLDIAQWGLGMDAS
                     GPVAIEGRGTFPKEKHLCEVTDTCRITYTYASGVTVVLGQKQKDIPDSVTFIGDQGQV
                     HVTRSKIEATPKELVDSKFTTSDTRLEVSRNHYQNFLDCIASRAKPIADVEIGHRTAT
                     VCHLGNIAVRLGRKFAWDPAKEQITGDADAAAMLTRPYRAPWKLA"
     gene            4430..5467
                     /locus_tag="VFB80_07905"
     CDS             4430..5467
                     /locus_tag="VFB80_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HZN33728.1"
                     /translation="MLRSVAIALAAVAAAASTVSAKPWAEKMFAVRTHDFGHVARGAK
                     AEFAFEIQNLYEEEVHIADVRTSCGCTTPAVTKNVLKTWEKGAIVATLNTRSFVGPRN
                     STLTVVIDKPFYAEVTLTISGTIHSDVDFQPGSVDLGEVEQGAGVEKEIIVTHRGRGP
                     WQISDIRSASNHLEVELSEPMRQPGQTTYKMLVRLKPDAPAGLINEQLTVVTSDKSLP
                     AITLPVEGRVAPPLSVSPSPLLFGAVPAGQSATKQLVITGKQPFKLLSISSEPAGLQF
                     RAVPELIKKVHLVPVTITAPAQAGEMVYSITIETDLPEGGKATCQARATIGGGAPSEA
                     RAAGAGSPAPR"
     gene            complement(5469..5855)
                     /locus_tag="VFB80_07910"
     CDS             complement(5469..5855)
                     /locus_tag="VFB80_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN33729.1"
                     /translation="MLEAALAGCGGPNVAPVSGRVTLNGQPLAGAHVTFQPVSPGPNI
                     RPEVAGSAGRTDAAGRYTLRLVEPDRPGALVGEHRVAISTAAGPDSDGVAPKGERVPK
                     AWRDGSKRFEVPAGGTSAANFELKSP"
     gene            complement(6002..6961)
                     /locus_tag="VFB80_07915"
     CDS             complement(6002..6961)
                     /locus_tag="VFB80_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33730.1"
                     /translation="MRGRIDPPASGFTLVELLVVIAIIGVLVALLLPAVQTARESARR
                     TSCLNNLHQIGTAIQLYHDVQKIFPPGGVSTGPCCSTDSYSSWPIAILPFLDQKNLAA
                     RYSHGETNESLANEFVRQQFVPIYACPSEPRPKTKERPESGPANDLRLQYMPGSYRGV
                     GGRSDGSGWWDNYPQHLSLPRSWKGVFHVVDGFPVPTTPLRPETFASILDGTSHTIMV
                     GEYATKTRLRRRTFWAYSYGSYNKSDAVPQSRTLLADWDRCASVGGAGDIQACNHGWG
                     SFHPGVLNFLLCDGSIRTLPLSIDMNLFVESATIAGREVTQLP"
     gene            7102..7515
                     /locus_tag="VFB80_07920"
     CDS             7102..7515
                     /locus_tag="VFB80_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN33731.1"
                     /translation="MQLQHILETSLYVDDLARAEHFYVEILGLSLVSRQEGRHVFLRC
                     GQRMLLLFNPLGSRQSSDQFPPHGAFGPGHVAFGVAESSLDHWSAYLAERGIPIERVI
                     DWPQGGRSLYFRDPAGNSLELATPRIWGIAEATLG"
     gene            7550..8842
                     /locus_tag="VFB80_07925"
     CDS             7550..8842
                     /locus_tag="VFB80_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33732.1"
                     /translation="MTVFRFAASLLLLQLAAVAALSAQERGIAEFPKLSAERDWPWWR
                     GPTRNGIASQAAVPTKFGPASAVWKAPVPGRGHSSPIVVGDRVYLTTADMQVKSQSVL
                     AFDRRSGKQVWQVEISRGGFPARNHPKNTEATPTIASDGERLFVTFFHHEQIQATALD
                     LGGKQLWQKTAGPFNPKKYEYGYAPSPLVYRGSVIIAAEYDGKSFLLALARESGRELW
                     RTPRPFNISFSSPVVGHVAGRDQLLISGADHVTSYNPATGKQLWTAAGTTQATCGTLV
                     WEGDTVIASGGYPKSQTLAIKADGSGRVLWENNQNCYEQSLLAAGGYAYALTGRGILY
                     CWRISDGQEMWKERLQGPVSASPVLAGGHIYWANELGTLYVFRPSPEKCQLVAENQIG
                     DDAFPSPAICGGQLFLRVGHKTPTGRQEILYCFGSRAE"
     gene            complement(8918..9376)
                     /locus_tag="VFB80_07930"
     CDS             complement(8918..9376)
                     /locus_tag="VFB80_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33733.1"
                     /translation="MPDLWNQNVMRLMMRRERRKWITLVGLLTGLVGCGESGPKLEPV
                     SGKVLKDGKAMTNVSISMVPEGSGIAAIGTADSSGTIVVQTNGRSGAMQGTYKIGVTE
                     PTRPMTPQALASGSPPPLSFDRKYESPQTSGFEFKVPEGGGKFEFSVTAK"
     gene            complement(9403..10428)
                     /locus_tag="VFB80_07935"
     CDS             complement(9403..10428)
                     /locus_tag="VFB80_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33734.1"
                     /translation="MYRHRMLGFTLVELLVVIAIIGVLVALLLPAVQAAREASRRTGC
                     TNNAKQLGLALHNYHDVFKVFPINYSQSSQGPNGPRGNSGDNDSRQCSWMALILPYIE
                     QSNLYSSINWNLGMKDSGGRPTSNVAIAQTVVPVFRCPSDPSGSVGGGRYGTREWLGR
                     WPDATFGQFAGTNYKGCVGSDWNWGIFANPMGGTPHGLDGGNGMFVRDENVSNANPKD
                     LRNMSQVTDGLSNTFAIGEALPELCSHTWWYGMNASLATSAIPLNHYLRVFRSTGRGP
                     NYFGSQTLFAPFWDWNNNYSFASQHSGGGMFTLGDGSVRFVSENINLQTYRALGTMNV
                     GEVAAAE"
     gene            10995..12041
                     /locus_tag="VFB80_07940"
     CDS             10995..12041
                     /locus_tag="VFB80_07940"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33735.1"
                     /translation="MNAHSGRGRGGFTLVELLVVIAIIGVLVALLLPAVQAAREAARR
                     IQCTNNLKQLALAILNYHDVHNVFPPGHIEDPRAYAQNQASSDSVSNLSNWGIMTLPF
                     IEQQALFDRYNNNVNNGDRDAAAWAIQKNVHEARVAAHLCPSDPRRGRLTTVSWVPLL
                     GTRYRATSYRAIAGGANVEAAAVGNTDGAQMNWSYCSWVRTSSAIGNVNGKYVGAFPC
                     VTSMDGIPAKAQALRNVVDGTSNTIILGETHWDERDVGNHNAFFSVSYPNINHAMFYP
                     VAATYIQNYDTCRTYSGIYGKYPCTNGWPSFHPGGRNWGRADGSVSVTSHTADLLILN
                     GMMTLGGGEVVTIN"
     gene            12148..12690
                     /locus_tag="VFB80_07945"
     CDS             12148..12690
                     /locus_tag="VFB80_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33736.1"
                     /translation="MMLNRPFSCSALCAGIAALAALAFCLAGCGPGYKLVPASGTVTV
                     DGKPAEGVTVLYQGVAAAGKLEAGPPLMKGVTDAQGRYTMTTADLKTGGVVGAERDGV
                     VAGTYRVLMAMSEEQAPREEVPDALEKRGVPKSKRPKVVLPPGAYGGKITIDIPPGGT
                     DKADFELTTPRSGSARAGGR"
     gene            complement(12854..13570)
                     /locus_tag="VFB80_07950"
     CDS             complement(12854..13570)
                     /locus_tag="VFB80_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33737.1"
                     /translation="MVEVAGDTLRVEGDKANNTIAIVDNGAAGIVVTCDAVASPAATG
                     IDHVVVHTKDGNDILTYSRSAAGGNFTTDLDFSAHLGKGNDSFTANLNGNDLAAGADV
                     RFHVKGEQGADILTVNAGTAAAATDIALGASLSIKLDGGKEGDTIAVNTAGDVDGTLK
                     VRASGGKEDDTVAGNLVLNAGSTGSVKAKVKGGKDDDALTLNITGAGAATAQIDAVLD
                     GGQGTDTGIATANVVVKRIP"
     gene            complement(14343..14902)
                     /locus_tag="VFB80_07955"
                     /pseudo
     CDS             complement(14343..14902)
                     /locus_tag="VFB80_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            complement(14899..15855)
                     /locus_tag="VFB80_07960"
     CDS             complement(14899..15855)
                     /locus_tag="VFB80_07960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZN33738.1"
                     /translation="MTTSPPTLAERFAGCLLGLAVGDALGAHFETQPAEFIARRYRAP
                     RELLDDPPEGRYCYTDDTQMAIGVAETLIACGCIDEAEICRRFAANYVPSRGYGRGAR
                     VVLDAMVEGGDHRYWAENLFPGGSHGNGAAMRVAPVGLLFRDDPSALWTQARLSALPT
                     HVHPLGVEGAQVLALAVAHASRAENFARPEFFAALAESCQLLEYAGPLRRAARVEHLR
                     DLALFGNGIAAADSVVTAIACFGHTPDSYELTIANAILLGGDTDTIAAMAGAISGAFL
                     GRQAIPQFLIDRLEDGYQGRTYIEKLSAGLLAAYEKAKGQKP"
BASE COUNT         2784 a         5192 c         5157 g         2815 t
ORIGIN      
        1 tgggctgggc ccaggcgcag gtcgcggcgg cccgcggcaa ctgggttcag gttggcctgc
       61 ggaacaaccc ggccgtcggg tatctcggcc agcagctggg cagcggtggc ctcgccgagc
      121 agcacgcctt catgatcgac caggagatca tcaccgccgg caaacggcag ctcagccggg
      181 ccgaggccga gcagcgcgtg gccatcgccg agcagcagct cgccgcccag cagcagcgcg
      241 tgctgaccga cgtgcggctg gcgttttacg acgctctcct ggcccagcag cgtctgaagc
      301 tggcgcagga gctgctcgcg atcgcccagc tggggcaggc cgcggccgat cgcctgcaca
      361 gggcgggcga aacgtcgcgg gtcgatctga tccagtcgaa cctggaagtc gagtccgccg
      421 aaatcgagct gaacaaggcg accaaccagc acacgtacgt ctggatgggt ctctcggcga
      481 tcatcggcct gccgcacctg ccgccggcgg tgctgcgcgg cgatctcgac gcggtgccgg
      541 cggagttgac ctgggaccag acgctggccc gcctcctgac ggccagcccg gagctggccg
      601 ccgcggcgag cgagatcgag cgggcccgct gggggctggc gcgggcccag cggcaggtga
      661 ttcccaacgt gcgattccag ggcgccgtga tgcaggacaa cggcatcggc ggcaagaccg
      721 acggcatcgt gcagctgctg gtgccgctgc cgattttcaa ccgcaatcag ggggcgatcc
      781 agcaggcccg cgcggaggtc gttgcggcgg agcgggcgat ccagcagctg gagctggacc
      841 tgcaataccg cctcgcgccg gtctatcagc ggtacgcgac cgcggccagc caggtccgcc
      901 gctaccgcga gcggattctg cccaaggccc aggagtcgct cgggctggtc cgccgcggct
      961 acgaggcggg cgagtttccc tatctgctgc tgctcaattc gcagcggacc tactttcaga
     1021 cgaacctcca gtacctcgaa tcgcttcgcg agctgtgggc ctcgtcggtc gagatcgacg
     1081 gcctcttact gcgcgacagc ctcagcgcgc ggccctaggc gctttgctat gattctgtag
     1141 gaatgccctc tgtggcattc ccaggagcta tcttcttgcc cgccgaatcg acgacgcaga
     1201 ttctcgcccg ccgctacgac acgctgcgtc cggtcgcgct ggaaatcgcg ggcgggcgaa
     1261 tcgcgaagat cagtccggcc gccggcggag cggagctgcc gatcgtcgcg ccgggcctcg
     1321 tcgatttgca aatcaacggc tacggcggca tcgagttcaa cgatccgcaa ttgactatcg
     1381 acaaagtgcg gcaagtggcc cgctcgcagg accccttcgg tgtgacagcg ttcctggcga
     1441 cgctcacgac cgacagccac gcggtcttgg cgcacgggct ggcgacaatc gccgcggcga
     1501 tcggcgaact gcccgaagtc gcgacgcgga ttcccgggat tcacctggaa ggacctttca
     1561 tctgccccga cgacggcccg cgcggggcgc accccaagca gcacgtccgc ccgcccgatt
     1621 gggacgagtt tcgccggctc caagatgccg cggcagggcg gatcaagctc cttaccatct
     1681 cggccgagta cgcctcatcg cccgacgtga tccgccgcgt cgccgagagc ggcgtgctgg
     1741 tcgcgatcgg ccacacgcag gccagcggcg aacaaattca ggctgccgtc gacgccggag
     1801 cccggatgag cacgcacctg ggcaacggct cgcaccccat gatccgccgc catccgaact
     1861 acatctggga tcagctggcc gaggaccgcc tggtcgccag cctgatcgtc gatgggcacc
     1921 atttgccgcc ggccgtagtc aagtcgatgg ttcgggccaa gacgccgccg cacgtggtcc
     1981 tggtcagcga catcacgtcg atgggcggca tgccgccggg caagtatcag acgggcctgg
     2041 gcgaactgga ggtgctgccc agcggcaagc tggtgcctgc cggccggccg ggaatcctcg
     2101 cgggggcttc gctgccgatc cacgtctgcg tggccaacgt catgcggttc gccggcgtcg
     2161 atctgaggac ggcgattgat atggccagcg tcgcacccgc gcgcctgatc ggcctggatc
     2221 atccaggcct ggaagtcggc gcaccggcca acctctttct gttcgatcta ccggcgagcg
     2281 aagcagcgcc gctgcaagtc cattcgacct ggcacgaggg tcggcggtcc catccgcact
     2341 agccaattca gacatgccca ttcaggacta tttcgtcggc ggcgctgccg cgctcttggg
     2401 cggctttctg ctgctgggcg cggcgctgga tgccgcctgg ctgatgagcc tgaagcggcc
     2461 gcgtctcttg gccgagtcga tcggcaaggc agcggcccgc tggacgctcg gcctggtcgg
     2521 ggtcggcctg atcgcgctgg gcgccctcat cgccagcggc tggcgcgtcg attggtcctg
     2581 gctcggtcag cgaaccgttt catccgcgcc gtcgcttcgc tgctcaaatc cgctgcaccg
     2641 gacgatcggc tgcggcgggt acttggcaaa ttgcgggtcg ctctgcgact tctggcagag
     2701 caggaaccgc gaggagcgcc cggagacgat ctcccgcatc cacgcgcagc ttcggcacag
     2761 cgagcggctt gcgaaatccc cttggttcat ctaggccagc ttccacggcg cgcgataggg
     2821 ccgcgtgagc atcgcggcgg cgtcggcgtc gcctgtgatc tgctccttgg ctggatccca
     2881 ggcgaacttc cgccccaggc ggacggctat attgcccagg tggcagacgg tggccgtgcg
     2941 gtggccgatc tcgacgtcgg cgatcggctt ggcccgcgag gcgatgcagt ccaggaagtt
     3001 ctggtaatgg ttcctgctga cctccagccg cgtatcgctg gtggtgaact tgctatcgac
     3061 cagctccttg ggcgttgcct cgatcttgct gcgggtcacg tgaacctggc cctgatcgcc
     3121 gatgaacgtg acggagtcgg ggatgtcctt ctgcttctgt ccgaggacga ccgtgacgcc
     3181 gctagcgtag gtgtacgtaa tgcggcaggt atcggtgacc tcgcagaggt gcttctcctt
     3241 ggggaaggtg ccgcggccct cgatcgcaac cgggccgctg gcgtccatgc ccaggcccca
     3301 ctgggcgatg tcgaggtggt gcgcgccaaa gttcgtcatc tgcccgccgc tgtagtccca
     3361 gaagaagcgg aacttgtagt ggacgcggtt gacgttgtac ggccgctgcg gcgctgggcc
     3421 aagccacagg tcgtagttca gctccggcgg cggatcgctg tcgggtgcgg ccgtggtgtc
     3481 tttcttgtcc acgggtgaca tgtggttggg tgcgggaatg ccgaccagca cggtctgcag
     3541 cttgccgagc ttgccattgc gggccagctc gcaggccagg cggaagcggt cgtcgctgcg
     3601 ctgctgcgac ccagtctgca cgacgcggcc ggtctcgcgg gcgacctcga ccatcttgcg
     3661 gccctcggtg acgaacagcg tgagtggctt ctcgcaatag acatccttgc cggcgcggca
     3721 ggcatcgacc gtcatcaggg cgtgccagtg atcgggcacc gagagcacca ccgcgtcgat
     3781 gtctttccgc tccagcagct ggcgatagtc gccaaagacg ctcggcttct gcccggcctc
     3841 ttcgacccgc ttcaccgccg aggcaaagac gtttttatcg acgtcgcaga ccgccgcgca
     3901 gtcggccctg cgattggccg cgccgcggcc aacgatgcca aagccctcca tgttctgcat
     3961 cccgcggttc ttcacgccca caaagcccag cacgacgcgc tcgttagccc caaaggcccg
     4021 ctgcgggatg aaatacgggg cggcgaagac tgcggaagcc gcggcggtcg tctgcaggaa
     4081 acgtcggcgg gagagcctgg acatgaaatc gatccttaag tgattgcgaa aacggcgggt
     4141 ccgttgatcg taactcatcg tgctgagtct cgctatcgat ggcgctgcca gcacgcgact
     4201 tgagccaaag ccgggccgca aactcgccgc agctttccca ccttcggcac aacccgaaaa
     4261 gcttgcagcg tagggtttcg ccggccgaaa taaaaggaag tacggacgcg tgcgcgccgc
     4321 ctgggcgagc tagtcgccaa ccggccgccg ccagccttag aatgccgact taggatgaaa
     4381 gtgacgcgct gctgcggagt tcgagccgcg gctggttgag ggggagagca tgctgaggag
     4441 tgtggcgatt gcgctggccg ctgtggcggc cgccgcttcg acggtcagtg ccaagccgtg
     4501 ggccgagaag atgttcgccg tgcgcacgca cgacttcggc catgtagccc gcggagcgaa
     4561 ggctgaattt gccttcgaaa tccaaaacct ctacgaggaa gaggtccaca ttgcagacgt
     4621 ccgcaccagc tgcggctgca cgacgccggc agtgaccaag aacgtcctca agacgtggga
     4681 gaagggggcg atcgtcgcca cgctcaacac ccgctcgttc gtcggcccgc ggaactcgac
     4741 gctgacggtc gtcatcgaca agccgtttta cgccgaggtc acgctgacca tcagcggcac
     4801 gatccactcg gacgtcgatt ttcagcctgg ctccgttgac ctgggtgaag tcgagcaggg
     4861 ggccggcgta gaaaaggaaa tcatcgtcac ccaccgcggc cgcgggccgt ggcaaatcag
     4921 cgacatccgc agcgccagca accacctgga ggtcgagctg agcgagccga tgcggcagcc
     4981 gggacagacc acgtacaaga tgctcgttcg cctgaagccc gacgcccccg ccggcctgat
     5041 caacgagcag ctgaccgtcg tcaccagcga caagagcctg ccggcgatca cgcttcccgt
     5101 cgaaggccgc gtcgcaccac cgctttcggt cagcccctcg ccgctcttgt tcggcgcggt
     5161 gccggcgggc caatcggcga ccaagcagct cgtgatcacc ggcaagcagc cgttcaagct
     5221 gttgtcgatt tccagcgagc cggccggact gcaattccgg gccgtaccgg agctgatcaa
     5281 gaaagtgcac ctcgtgcctg tgacgattac cgcgccggcg caggccggcg agatggtcta
     5341 ttcgatcacc atcgagaccg atttgcccga gggcggcaag gcgacttgcc aggcccgggc
     5401 caccatcggc ggcggcgcgc cgagcgaggc ccgcgcagcc ggcgccggca gtcccgcgcc
     5461 tcgctaggct acggactttt cagctcgaag ttcgccgcgc tggtgccgcc cgcagggact
     5521 tcgaatcgct tggagccgtc gcgccacgcc ttgggcactc gttccccctt gggcgctacg
     5581 ccatcggagt ccggcccggc cgcggtcgag atcgccaccc ggtgctcgcc gaccagcgcg
     5641 cccggccggt cgggctcgac tagccgcagc gtgtagcggc ccgcggcgtc cgtccgtccc
     5701 gcggagcccg cgacttccgg gcggatgttg ggaccagggc tgaccggctg aaaggtcacg
     5761 tgcgccccgg ccagcggctg accgttcagc gtgacgcgcc cggaaaccgg cgcgacgttg
     5821 ggcccgccgc aaccggccaa agcggcctcc aacataagcg cagcgaaggc gacgaatcgg
     5881 tcgtaacgag ggccggccat aggcactaca ctaacccgac gcgtaagcga gggactccgt
     5941 cgaattgcgt cgcctcagaa agtccctcgc tgacgcctcg ggttggtatc ggcagacatc
     6001 cctacggcaa ctgcgtcact tcgcggccgg cgatcgtcgc cgattcgacg aacaggttca
     6061 tgtcgatcga cagcgggagc gtgcgaatcg aaccgtcgca gagcaggaag ttcaggacgc
     6121 ccgggtgaaa gctgccccaa ccgtggttgc aggcctgaat gtctcccgcc ccgccgaccg
     6181 acgcgcagcg gtcccagtcg gccagcagcg tgcggctctg gggcacggca tcggacttgt
     6241 tgtacgagcc gtaagaatag gcccagaacg tgcggcggcg cagccgggtc ttcgtcgcgt
     6301 attcgccgac catgatggtg tgcgacgtgc cgtcgaggat cgacgcaaaa gtctccggcc
     6361 gcagcggcgt ggtcggcacg ggaaagccat cgaccacatg gaacacgccc ttccagctcc
     6421 gcggcaagct gaggtgctgc ggatagttgt cccaccagcc gctgccgtcg ctccgcccgc
     6481 cgacgccgcg gtaggaaccg ggcatgtact gcagccgcag gtcgttcgcc ggaccggact
     6541 cgggccgctc cttggtcttg ggccgcggct ccgaggggca ggcatagatc gggacgaact
     6601 gctgccgtac aaactcgttg gccagcgact cgttggtttc gccatggctg tagcgggcgg
     6661 cgaggttttt ctggtccagg aatggcagaa tcgcgatcgg ccacgacgaa tagctgtcgg
     6721 tcgaacagca gggcccggtg ctcacgccgc cgggcggaaa gattttctgc acgtcgtgat
     6781 agagctggat cgccgtgccg atctgatgca ggttgttgag gcagctcgtc cgccgcgccg
     6841 actcgcgggc cgtctggacc gcgggcagca ggagcgccac cagcaccccg atgatcgcaa
     6901 tcacgaccag cagctccacg agcgtaaagc cgctcgcagg cgggtcgatt cttccgcgca
     6961 tcgccgcatc ctctcagctc ggcaggcgcg gtggatgggc cgaccggaga atcgcccgcc
     7021 gtaagctgaa ctgttaccat aacgggggac tgatgggttg acaactctcg cctcgcatcg
     7081 gccggcggcc gttacattcc catgcagctg cagcacatcc tggaaacttc gctctacgtc
     7141 gatgacctgg cgcgggcgga gcacttctac gtcgaaatcc tcggcctgtc gctcgtcagc
     7201 cggcaggagg gccgccacgt ctttctccgc tgcggccagc ggatgctgct gctgttcaac
     7261 cccctcggca gccgccagtc gagcgaccag ttcccgcccc acggggcgtt tgggccggga
     7321 cacgtggcgt tcggcgtggc cgagagctcg ctcgaccact ggagcgccta cctggccgag
     7381 cgcggcatcc cgatcgagcg ggtgatcgac tggccgcagg ggggccggag cctctatttc
     7441 cgcgatccgg ccggcaacag cctggagctg gccacgccgc ggatttgggg catcgccgag
     7501 gcgacgctcg gctgacgcaa cttcgacttt cataccatcg gagcgtttga tgactgtctt
     7561 tcgctttgcc gccagcctgc tgctcctgca actggcggcc gtggctgctc tctcggcgca
     7621 agagcgcggg atcgccgagt ttccaaaact ctcggccgag cgcgactggc cctggtggcg
     7681 gggaccgacg cgaaacggca tcgccagcca ggctgcggtg ccgacgaagt tcggcccagc
     7741 tagcgcggtg tggaaagcgc cggtgccggg ccgcgggcac agctcgccga tcgtcgtggg
     7801 tgatcgcgtc tatctgacga ccgccgacat gcaggtcaaa agccagagcg tcctggcatt
     7861 cgatcgccgc tccggcaagc aggtctggca ggtggaaatc agccgcggcg gcttcccggc
     7921 ccgcaatcat cccaagaaca ccgaggccac gccaacgatc gcgtccgacg gcgagcgctt
     7981 attcgtcacg ttctttcacc acgagcagat tcaggccacg gcgctcgacc tgggcggcaa
     8041 gcagctctgg cagaaaaccg ccggaccctt caatcccaaa aagtacgagt acggctacgc
     8101 cccctcgccg ctggtctatc gcggctcggt gattatcgcc gccgagtacg acggcaagag
     8161 ctttctcttg gccttggccc gcgagagcgg ccgcgagctg tggcgcacgc cgcggccgtt
     8221 caatatcagc ttttcgtcgc cggtcgtggg ccatgtcgcc ggccgcgacc agctgttgat
     8281 cagtggggcg gaccatgtga cctcgtacaa cccggcgacc ggcaagcagc tctggacggc
     8341 cgccggcacg acgcaggcta cctgcggcac gctcgtctgg gagggggaca cggtgatcgc
     8401 cagcggcggc taccccaagt cgcagacgct ggcgatcaaa gccgacggca gcggccgcgt
     8461 gctgtgggag aacaaccaga actgctacga gcagtcgctg ctggccgccg gcggctacgc
     8521 ctacgccctc accggccgcg gcatcctcta ctgctggcgg atcagcgacg ggcaggagat
     8581 gtggaaagag cggctgcaag gcccggtgag cgcctcgccc gtgctggcgg gcggccacat
     8641 ctactgggcc aacgagctgg gcacgcttta tgtcttcaga cccagcccgg aaaagtgcca
     8701 gctcgttgcc gagaaccaga tcggcgacga cgccttcccc agccccgcca tctgcggcgg
     8761 ccagctcttc ctccgcgtcg gccacaaaac gccgactgga aggcaggaga tactgtactg
     8821 ttttggatcg cgggccgagt agcccgataa gaagtagaat gtcccgtaga atgtcccttt
     8881 ttccgccccg cgttctggac gggaaaggga cttggattca tttggccgtc acgctgaact
     8941 caaattttcc accgccctcg ggtactttga attcaaagcc gctggtttga ggcgactcgt
     9001 acttgcgatc gaagctcagc ggaggtggac ttcctgacgc cagcgcttgg ggagtcatcg
     9061 gacgtgtcgg ttcggtaaca ccgatcttgt acgttccttg catggctccc gatcgtccat
     9121 tggtttggac gacgatcgtt ccggagctat ccgcggtgcc aatggctgca atgccggacc
     9181 cttccgggac catggagatg gaaacgttgg tcattgcctt tccgtccttc agaaccttgc
     9241 cgctgaccgg ctccagcttt ggcccacttt caccacagcc cacgagtccg gtcagaagcc
     9301 cgacaagggt gatccatttc cttcgttccc tacgcatcat caacctcatg acgttttgat
     9361 tccaaagatc cggcacgact cgctccgcag gaaacgaaat cgctattccg cggccgcgac
     9421 ctcgccaacg ttcatcgtcc ccagtgcccg gtacgtctga aggttgatat tctcactaac
     9481 gaatcgaacc gaaccatcac ccaatgtaaa cattccgccg ccgctatgct gactggcaaa
     9541 gctatagttg ttgttccagt cccagaacgg cgcgaacagc gtctgactgc cgaagtagtt
     9601 tgggccgcga ccggtgctcc taaacactcg caggtagtga ttcaagggta ttgccgaggt
     9661 cgccaatgag gcgttcatcc cataccacca ggtgtgcgag cagagttccg gcagggcttc
     9721 tccgatggcg aacgtgttcg aaaggccgtc cgttacctgg gacatgttac gtaaatcctt
     9781 gggattggca ttactcacgt tctcgtcgcg tacaaacatg ccattgccgc catccaaacc
     9841 atgcggagtt ccacccatgg gattggcaaa aatgccccag ttccagtcag atcccacaca
     9901 gcctttgtaa ttggttccgg cgaattgtcc aaaagtggca tccggccaac gcccgagcca
     9961 ttcacgggtt ccatatcgcc ctcccccaac ggatccgctc gggtcagaag ggcatcggaa
    10021 gaccggaaca acagtctgag caatcgccac gttcgacgta ggacgtccgc ccgaatcctt
    10081 catgcccaga ttccaattga tcgagctgta gaggttcgac tgctcgatat agggcaggat
    10141 caatgccatc catgagcact gtcgcgaatc gttgtcgccc gaattgcctc gtggaccgtt
    10201 ggggccttgc gaagattgcg agtaattgat ggggaagacc ttaaaaacgt catggtagtt
    10261 gtgaagcgct agcccaagct gctttgcatt gttagtgcat cccgtgcgcc gtgacgcctc
    10321 gcgcgcggcc tgaacagcgg gcagcaacag cgccaccagc acgccgatga tcgcgatcac
    10381 cacgagcaat tcgacgagcg taaacccgag catgcgatgg cgatacatga tggagactcc
    10441 gcaaggaacg aaacgacccc caggaaacga gccgagttcg gcgatgcggc gctccgcggc
    10501 tgatcccgct tgttgggtgc agcgcgcgct ggagaaaacc gcccgatggt ccccacgaac
    10561 gcctgcccgc aaccctcatt atgccgccga tgaatgggcg gccagccaga caagccgcag
    10621 cgtaccggtt gcctctttcc tgccgctcgg ccagttgtta gcgtagcaat cgggaaaaag
    10681 aaaacaacgg ctgtgtggcg attgttcgtt tctggccggc gctctcggcc ggaggattca
    10741 gggggattag cgcaggaata ttcgacttgt cgccgttttc cggctccagc tattggctga
    10801 tgcctcttgc aggcccgagg gttgacgagc cgaccatcgc cgcgcgatgg tgcccggggc
    10861 cacccgcgtc ccggaaagcg caaattaggc cgccaccaag ccgcggccgg atttgacatt
    10921 gttttgtttc agcggcacaa tgcgccgacc ggggcagagc ggcttctctt cgtcgcctct
    10981 ataaggagcg ccgcatgaac gctcattctg gccgcggtcg cggcgggttc acgctcgttg
    11041 aattgctggt cgtcattgcg atcatcggcg tgctggtggc gctgctgctg ccggccgtac
    11101 aggcagcccg ggaagccgcc cggcgcatcc agtgcaccaa caatctcaag cagcttgcgc
    11161 tcgcgattct caactatcac gatgtccaca acgtcttccc gcctggacac atcgaggatc
    11221 cgcgtgccta tgcccagaac caggcaagca gcgactcagt ctccaacctc tcgaactggg
    11281 ggatcatgac gcttccgttc atcgaacagc aggcgttgtt tgaccggtac aacaacaacg
    11341 tgaacaacgg cgaccgcgac gccgcggcct gggccatcca gaagaatgtc cacgaagccc
    11401 gcgtggccgc tcacctctgc ccctcggacc ctcgccgcgg gcgcctcact acagtcagtt
    11461 gggtcccgtt gcttgggact cgctacaggg cgacctcgta tcgtgccatc gccggcggcg
    11521 ccaatgtgga ggctgcggcg gtcggtaata cggatggcgc ccagatgaat tggtcctact
    11581 gctcttgggt ccgcacctcg tcagcgatcg ggaacgtgaa tgggaagtac gttggcgcgt
    11641 ttccgtgcgt gacctccatg gatggcattc ccgccaaggc ccaggccctc aggaacgtgg
    11701 tggacgggac cagcaacacg attatcctgg gcgagaccca ctgggacgag cgggacgtgg
    11761 gtaaccacaa cgcgttcttc tcggtgtcct acccgaatat taaccacgcc atgttctacc
    11821 cggtggccgc gacctacatc cagaactacg acacgtgcag gacttactcc gggatttacg
    11881 ggaagtatcc atgcacgaac ggctggccct cgtttcaccc gggcgggcgg aactggggga
    11941 gggccgacgg ctccgtgtcc gtcacctcgc ataccgccga cttgctgatc cttaacggca
    12001 tgatgacgct cggcggcggc gaggtggtga ctatcaattg agctaggacc gttctggtcg
    12061 tgctggtttt cgtacgtgcg ccggtgggaa gagtgccttc ccggcggatt gcgctggcgc
    12121 gttcctccaa gttcccgagg tttcaagatg atgctgaaca ggccgttctc gtgttcggcc
    12181 ctttgcgcag gaatcgcggc gctggcggcc ctcgcttttt gtctggcggg ttgcggccca
    12241 gggtacaagc tcgtgcctgc ctccggaacg gttaccgtgg acggaaagcc ggcggaaggg
    12301 gtgacggtcc tctaccaagg ggtcgccgcg gcgggaaagc tcgaggcggg cccgcctctg
    12361 atgaagggcg tgaccgacgc gcaaggtcgc tacacaatga ccaccgcgga cctcaaaacc
    12421 ggcggggtgg taggcgccga gagggacggt gttgtggcgg ggacgtatcg ggttttgatg
    12481 gcgatgtccg aggaacaggc gccacgagag gaagtgccag atgccctcga aaaaaggggc
    12541 gttcccaagt cgaagaggcc caaggtcgtt ctgcctcccg gggcttacgg cgggaagatc
    12601 accatcgaca ttcccccggg cgggacggac aaggcggatt tcgagctaac gacgccccga
    12661 tcagggtcgg cacgtgccgg tggcagatga caggtgcgag cgggaggagg ccgtgggtcg
    12721 ggaatcggga gtcggggagc accctcaagc ctgccccctc ttccagtttc gccgcgcgca
    12781 ttaagacatc gtgcgctcgg gcgaagcccg agcacgtgta tgttccgcaa gacggaccca
    12841 catcgctacc ggcttagggg atacgtttca ccaccacgtt ggcggtggcg atgcccgtat
    12901 cggtgccctg gccgccgtcg agcactgcgt cgatttgggc cgtggcagcg ccagcgcccg
    12961 taatgttgag ggtcagggcg tcgtcgtcct tcccgccttt gaccttggcc ttgacggaac
    13021 cggtgctccc ggcgttcagg acgaggttgc cggccacggt gtcatcttcc ttgccgccgc
    13081 tcgcgcgaac cttgagcgtg ccgtccacgt cgcccgcagt gttcaccgcg atcgtgtccc
    13141 cttccttgcc gccgtccagc ttgatactca ggctggcgcc cagggcgatg tccgtggctg
    13201 ccgcggcagt gcccgcgtta accgtcagga tgtcagcgcc ctgctccccc ttcacatgga
    13261 accggacgtc agcgccggca gccaggtcgt tgccgttcaa attggccgta aacgagtcgt
    13321 tccccttgcc caggtgggcg gagaaatcga ggtcggtcgt aaagttgcca ccggcagccg
    13381 accgcgagta ggtgaggatg tcgttgccat ccttggtgtg gacgacaacg tgatcaatac
    13441 ctgtcgcagc cggagaggcg acggcgtcgc aggtgacaac gatccccgca gcgccgttat
    13501 cgacaatagc tatcgtgttg tttgccttgt cgccttcgac ccgcagcgtg tctccggcga
    13561 cttcgaccac acaggtcagg cagcaacgat tttccaacgc ttcgagtcgc ggcacatact
    13621 tgtgccccgg gcgagaccga ccagagtgac gcgagtacat ggcaactcct caaaaagggg
    13681 acatgatgta acgccgctct ggacgcgagc gacggctaac ctcgctcaac gcggtagcac
    13741 ggaccccaag cacccccaaa cgatttacag cggggcaact cgaaacaccc gctaaagctg
    13801 gcaattcgag gcaactggcc gggctgcaaa aacgcggcaa cttccgcaat cggtatgcca
    13861 gtgcctgctc acgcgctacc ggcttggctc cttcagtcga tcattgcgag ggttcctgaa
    13921 aatggtgcgc ccgtcaggca ttctgtccgt gctgtgtgac aagctgtccc tgcagtgccg
    13981 ccgaacgcgg tatgaaacca agccgcgaat ctgccgatgt gggccggtgt ccgacacagg
    14041 ctagcgttct ccggctacat cgtctattga caattagtga acacccgata caattacggt
    14101 tagcgcgtgg cggctgcttg ctctttggca tcttggttgt tggcaatcag ccgaaatccg
    14161 tgtcagcgcc cttcaggtgc aaggcacccg ccggcgcgca taatgcgcat aatgggtttt
    14221 gggcagattt ggtcgtaagg cattgtgacg actggagttg tggcttgaat ccgccctcca
    14281 agaaaacttc gcaatatgcg cgcatcgctc gggcgggctg atcgcggatt gcgttgcccg
    14341 catcagtttt cttcgtcctc ggcaaccaac agatattccg gcggaacgtg gtccgtgagc
    14401 cagacctggt tgccggtgag gaagaagggg tggcccgcgg cgtgcatgcg ggcagcggcg
    14461 atggcaagca caacgggctg gccatggcgg gttgcgacct gaatcatcgt ctgtcggttg
    14521 gtggagagat gaacgtggtg ccggcggcct ctattgaggc cggtccgcag gatcgattcg
    14581 aggttgtgcc gcgccgtgcc gtggtagagc acgtcgggcg gaactgccgg ctcgtagccc
    14641 aggtccactt ccactgaatg cccctgccgg gcgcggattt ggccccgtcc tcgctcagct
    14701 cgaaccgctg cttgtcgctg gtcgccacga cttccgccag cgcctcgcga ggtaccggct
    14761 cgccttcgcg ggccagggcc gcctggagta cttcgacctc cacccagccg ccctcttcga
    14821 gctggatgcc gaccgagtcc ggccggtgcc gcaggacgta gctgaggcgc ttgctgattt
    14881 gcgttcgctc cgcggcgttc acggtttttg tcctttcgcc ttctcgtacg cggccagcag
    14941 gccggcagac aatttctcga tataggtgcg gccttgatag ccgtcctcca ggcggtcgat
    15001 gaggaactgc ggaatggctt gccggcccag gaacgcgccg ctgatcgcac cggccatcgc
    15061 ggcgatcgtg tcggtgtcgc cgcccagcag aatggcattg gcaatcgtca attcatacga
    15121 gtccggcgta tggccgaagc aggcgatcgc cgtgaccacc gaatccgcag cggcgattcc
    15181 gttgccgaag agggccaggt cgcggaggtg ctcgacgcgg gccgcccgcc gcagtggtcc
    15241 ggcatactcc agcaactggc agctctcggc cagggccgcg aagaactccg gcctggcgaa
    15301 gttttcggct cgcgaggcgt gcgccacggc cagggccagg acctgcgccc cctcgacgcc
    15361 cagcgggtgc acgtgcgtgg gcagggccga caggcgggct tgggtccaca gggcgctcgg
    15421 atcgtcgcgg aacagcaggc cgacgggcgc gacccgcatc gcggccccgt tgccgtgcga
    15481 gcccccggga aacaggttct cggcccaata gcggtgatcg cccccttcca ccatcgcgtc
    15541 gagcacgacc cgcgcgccgc ggccgtagcc gcgggacggc acgtaattgg ccgcgaaccg
    15601 gcggcagatt tccgcttcgt cgatgcagcc gcaggcgatc agcgtctcgg cgacgccgat
    15661 cgccatttgt gtgtcgtcgg tgtagcagta gcggccctcc ggcggatcgt cgagcagctc
    15721 gcgcggcgcc cgatagcggc gggcgatgaa ttccgccggc tgggtctcga agtgagcccc
    15781 cagcgcatcg ccgaccgcca ggccgagcag gcagcccgcg aagcgttcgg cgagcgtcgg
    15841 tggcgaagtc gtcatggcga ttagctgcgt ttctgcagtt ccgcatcagc cgcaaacaat
    15901 cctgcaaaca gtttgaggtc caccggtttt cgctgcccgg cgagccac
//
LOCUS       DASRJB010000295         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_646589, whole genome shotgun sequence.
ACCESSION   DASRJB010000295 DASRJB010000000
VERSION     DASRJB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646589"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFB80_07965"
     CDS             complement(<1..251)
                     /locus_tag="VFB80_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HZN33739.1"
                     /translation="MSAAETIDFTTEPLTSQQPAEAVARVVREAVGRQASDLFLFSDE
                     SAVNIAVRTLGHVERLATVSRESGRHLISYFKTLAGIDIA"
     gene            complement(248..598)
                     /locus_tag="VFB80_07970"
     CDS             complement(248..598)
                     /locus_tag="VFB80_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN33740.1"
                     /translation="MKLSIAQDDGNQVCVAISGQVTQREVSPIEEPLALLLGPTAYSR
                     LVRLDLSNASFMDSSGVGWLLTCQKRMRQAGGKLTLFSPHPIVANVLKVLKLERVFDI
                     EQAAAARGPAGGGS"
     gene            complement(701..994)
                     /locus_tag="VFB80_07975"
     CDS             complement(701..994)
                     /locus_tag="VFB80_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZN33741.1"
                     /translation="MSAYELPTIDQEVCRGCGACCLRQPWPPFLDEHELAAIPRRLRE
                     ELKRASRDRTGLPCFWLDLLTHECRHYEHRPVACREFQPGSENCLEDRAACGL"
     gene            complement(991..1524)
                     /locus_tag="VFB80_07980"
     CDS             complement(991..1524)
                     /locus_tag="VFB80_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN33742.1"
                     /translation="MTAGIVRIGESPRALEIQVEGRGTMLHSQAIRQWAEVRVLSIAR
                     VRVDLSRCTHMDSTFVGTLLCLKRLSESRPGGGLVLVGPSVQCLQVLEQMRIGRIFAV
                     DPECPVGCDWQEVQIDPCAAKTLAFKSTVVEAHQHLADCPGPAGDRFRQLADDMAREL
                     ARETAGKTAQPGERGSA"
     gene            1668..1880
                     /locus_tag="VFB80_07985"
     CDS             1668..1880
                     /locus_tag="VFB80_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33743.1"
                     /translation="MADVPQAEAHGEHDIWEHMTPDMRAELKSEDSTAWRQVVGVLLL
                     IISIGLVLAVFTAATSGLFTSSLRSL"
     gene            1972..>2962
                     /locus_tag="VFB80_07990"
     CDS             1972..>2962
                     /locus_tag="VFB80_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33744.1"
                     /translation="MRSLVCAPLVAALLFSPALVEAQVPYGPGDWPQWRGPRGDGISL
                     DSGLLKEWPQEGPPVVWRVDSVGVAYSSIAVKDGRIYTQGDLGGVEHVICLDATGGQT
                     IWAVQPEPVARLLAARVAGELKQLDKNGDGKVDELEALTRFGWSWNQYNQPISGDLAA
                     NAKQRATALFNQLDKNMNGKLEFAEAGNLLREQFERIDAEDKSADGAQLAAARSGAHF
                     TSLDKDGDGRISRPEARGSALDRNFSRYDARDPATNKGDELLSSDELTQGLLKSEPGR
                     DGEITAEELERFYIQSNVKGDGELVVSELAGTVGGYRNGMGDGPRGTPTVDGDR"
BASE COUNT          503 a          957 c         1032 g          470 t
ORIGIN      
        1 gcaatgtcga tccccgccag cgtcttgaag tagctgatga ggtgccggcc ggattcgcgg
       61 ctgacggtcg ccagccgctc gacgtggccg agcgtccgga cggcgatgtt gacggcgctc
      121 tcgtcgctga acaggaacag gtcgctggcc tgccggccga ccgcctcgcg aacgactcgg
      181 gcgacggcct ccgccggctg ctggctggtg agcggttcgg tggtgaagtc gatcgtctcg
      241 gcggcgctca tgagccgcct cccgccggcc cgcgcgcggc cgcggcctgc tcgatatcga
      301 aaacccgctc gagcttgagc accttcagca cgttggccac gatcgggtgt ggcgagaaca
      361 gggtcagctt gccgccggcc tgccgcatcc gcttctggca cgtcagcagc cagccgacgc
      421 cgctggaatc catgaagctc gcgttgctca ggtccagccg cacgaggcgc gaatacgccg
      481 tggggccgag cagcagggcg agcggctcct cgatcgggct gacctcgcgc tgggtgacct
      541 ggccggaaat cgccacgcaa acctggtttc cgtcgtcctg ggcaatcgag agtttcatgc
      601 gagcgctatc gaatagaagg acggggcggg gacgggcggc tgcaatgtac cggccaaggc
      661 cgccggcagc aataaatccc gctcggaccg caccgaaaat tcacaagccg caggcggcgc
      721 ggtcttccag gcaattctcg ctgccgggct ggaactcgcg gcaggcgacc gggcggtgtt
      781 cgtagtgccg gcattcgtga gtcagcagat cgagccagaa gcaaggcaat ccagtgcgat
      841 ctcgcgaagc acgtttcagt tcctcccgca gccggcgggg aattgccgcc agctcgtgct
      901 cgtcgagaaa cggcggccag ggctgacgca agcaacacgc cccgcagccg cggcagactt
      961 cctggtcgat cgtgggcaat tcgtaggcgc tcacgcggag ccccgctcgc cgggctgcgc
     1021 ggtctttccc gcggtttcgc gggccagttc gcgggccatg tcgtcggcga gctggcgaaa
     1081 gcgatcgccg gctgggcccg ggcagtcggc caggtgctgg tgggcctcga cgaccgtgga
     1141 cttgaaggcc agcgttttgg cagcgcaggg gtcgatctgg acctcctgcc agtcgcaacc
     1201 cacggggcac tctgggtcca cggcgaaaat ccggccgatc cgcatttgct ccaggacctg
     1261 caagcactgg acggatgggc cgaccagcac cagccctccg cccggccgcg actcgctcag
     1321 ccgcttcagg cacagcaggg tgcccacgaa agtgctgtcc atgtgggtgc agcggctgag
     1381 atcgacgcga acgcgggcga tcgacagcac gcggacttcg gcccactggc ggatggcctg
     1441 gctgtggagc atggtccccc ggccctcgac ctggatttct agcgcccttg gcgattcgcc
     1501 gatgcggacg atgccagcgg tcatgggaac ctcagtacga gtcggccagc aaccataccc
     1561 taccggattt gggcggtttg tcctaccgtg cagtggccaa aaccggtccg atcagtcaca
     1621 atggatgatt gttgcgtagg cactggcgac cgatcgggga gtagaccgtg gcagatgtac
     1681 cgcaggccga ggcgcatggc gaacacgata tctgggagca catgactccc gacatgcgcg
     1741 cggagctgaa gtcggaagac tcgaccgcct ggaggcaggt ggtcggcgtg ctgctcttga
     1801 tcatctcgat cggcctggtg ctggccgtgt tcactgcggc gaccagcggt ctgttcacgt
     1861 cgtcgctgcg ttcgctgtga cccttcggct ggcgcgcgaa agagcgccag ccgcctgccc
     1921 gttcgatccc gccaccgcat cccacccaaa acacctgccg gagtttgcga catgcgatcg
     1981 ctcgtttgcg cgccgctagt ggctgcgctg cttttctcgc ccgcgttggt tgaggcccag
     2041 gttccctatg gccccggcga ttggccgcag tggcgcgggc cgcgcggcga cggcatcagc
     2101 ctcgattcgg gcctcttgaa ggagtggccg caggaagggc cgccggtcgt gtggcgggtc
     2161 gattcggtgg gcgtggccta ttcgtcgatc gcggtgaagg acggtcgcat ctatacgcaa
     2221 ggcgatttgg gcggcgtcga gcacgtgatc tgcctggatg ccacgggcgg gcagacgatc
     2281 tgggccgtgc aacccgagcc cgtcgcgcgt ctcctggccg cgcgagtcgc cggcgagctc
     2341 aagcagctcg acaagaacgg cgacggcaag gtcgatgagc tcgaggcgct gacgcggttt
     2401 ggctggagct ggaatcaata caaccagccg atcagcggcg acctggccgc gaatgcgaag
     2461 cagcgagcga cggcgctctt taaccagctc gacaagaaca tgaacggcaa gctggaattc
     2521 gccgaggcag gcaacctgct ccgcgagcag ttcgagcgga tcgacgccga ggacaagtcg
     2581 gccgatggcg cgcaactggc cgctgcccgc agcggcgctc acttcacctc gctggacaag
     2641 gacggggacg ggcggatttc gcggccggaa gcccgcggtt cggctctgga ccgcaatttc
     2701 agccgctacg acgcgcgcga tccggccacg aacaaggggg acgaactgct ctcgagcgat
     2761 gagctgacgc agggcctgct caagtcggag cccggccgcg acggcgagat caccgccgag
     2821 gagctggaga ggttctacat ccagagcaac gtcaaggggg acggcgaact ggtcgtcagc
     2881 gagctggccg gcacggtcgg cggctaccgc aacggcatgg gcgatggacc ccgcggtacg
     2941 cccacggtcg atggcgaccg gc
//
LOCUS       DASRJB010000296         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_646619, whole genome shotgun sequence.
ACCESSION   DASRJB010000296 DASRJB010000000
VERSION     DASRJB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646619"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFB80_07995"
     CDS             <1..414
                     /locus_tag="VFB80_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33745.1"
                     /translation="SLSDGKVYEIFRQTKAQGKALGHAKKEDGPPGQAKKEDGARGHG
                     AKAENDAEGESPGLSRGRVSEQLLDRLAHSTSPTPRGNGVGAGRAASTGGETQARAAA
                     FAALADSDSDASGGGREFLPRLVSQRLLKRLARGG"
     gene            complement(418..2058)
                     /locus_tag="VFB80_08000"
     CDS             complement(418..2058)
                     /locus_tag="VFB80_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN33746.1"
                     /translation="MKDSSVTSALHRAVCLLVLVCARPALAELRFDVSFDASVDPGPL
                     TGRLLVIIAKEGSREPRLLASSFAGSTPFFGVDVAGLAPGQSATIDERALGYPVSSLK
                     DLPAGDYSVQAVLNVYTQVRRKDGHTVWVHLDQWEGQQWNRSPGNLVSSVQRVRLDPA
                     AGFAVRLALDKKLPAVEVPPDTEWVKRVKIQSHLLSEFWGCPIYLGATVLLPRGYNQD
                     TEQKYPAIYVQGHFNLRPPLGFAPPGMAFGRGPEFYRAWTSDDFPRMVAVTFQHPTPY
                     YDDSYAVNSANNGPYGDALTQELIPYLEQKYRLLPQPEARVLTGGSTGGWEALALQIF
                     HPKFFSGVWSLYPDPVDFRRYQVGNVYEDANAFSVPRGDWARLERPLSRTIDGQVTQT
                     MREMSQAEAVLGSNVRSGGQIAAWDAAYGPVGKDGYPRPLWDRLTGKIDKDVAAYMRD
                     NGYDLRHYLEQNWTKLGPDLTGKIYVACGDEDTYYLNLAVYLLEDSLKLAKNPPASAI
                     FEYGRLKGHGWQPFSNADLVRKMAERISATSPGLVTTR"
     gene            complement(2055..3437)
                     /locus_tag="VFB80_08005"
     CDS             complement(2055..3437)
                     /locus_tag="VFB80_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN33747.1"
                     /translation="MRTASQIVAIVFIGVWALPAPAQEPKQPPSAETVAAQANEFLQA
                     QVAVNRFSGSVLVAKGEQVLLAKGLGLANVEHEIPNTPQTKFRLGSITKQFTAMAILI
                     LQEQGKLSVEDLASKHVEGTPEAWKEVTIHHLLTHTSGIPSFTGLPGYRAAMPLPSPA
                     AKTLDRVRELPLEFTPGEKFNYSNSGYVLLGQIIEKVSGKSYGEFLRESIFQPLGLAD
                     TGYDSPEKILPHRAAGYRREGDGVANAPYLDMTIPHGAGALYSTATDLHRWSQALASS
                     QLISSGAYEKMFTPGKSNYGYGWIIDKQFGRQRMQHGGGINGFSTFIARYPDEKLCVV
                     VLSNIEGTPAAMVASGLAAIALGEPYELPKERKFVKLDAKILDAYTGEYEIGPNLVLA
                     VTRDGDRLLAQPTGQPKAELHPQSDAQFFVKEVSAEITFVKDDEGQVTHLILKQGERE
                     TKAKRIKGTP"
BASE COUNT          626 a         1120 c         1192 g          645 t
ORIGIN      
        1 tccctgtccg acggcaaggt gtacgagatc ttccgccaga ccaaggccca aggcaaagcg
       61 cttggccacg ccaagaaaga ggacgggccg cccgggcagg cgaaaaagga ggacggagca
      121 cgcgggcacg gggcgaaggc tgaaaatgat gccgaaggcg agtcgcccgg cctctcgcgc
      181 ggccgcgtga gcgagcagct gctggaccgc ctggcgcatt ccacatcgcc aactccgcgg
      241 ggaaacggcg tcggcgccgg ccgcgcggca tccaccggcg gcgaaacaca ggcccgcgca
      301 gctgcatttg ctgcgctggc cgattccgat tcggacgcga gcggaggcgg ccgcgaattc
      361 ctgccgcgac tcgtcagcca gcgcctgctc aaacgcttgg cgcgcggtgg ataaagccta
      421 gcgcgtcgtc accaggcccg ggctggtggc gctgatccgc tcggccattt tgcggacgag
      481 atcggcgttc gaaaacggct gccagccgtg gcctttgagg cggccgtact cgaagattgc
      541 gctggccggc ggattctttg ccagcttgag cgagtcctcc agcaaataca cggcaaggtt
      601 gaggtaatag gtgtcttcgt cgccgcaggc cacgtagatc ttaccggtca gatcgggtcc
      661 gagcttcgtc caattctgtt cgaggtagtg ccgcaagtca tacccgttgt cgcgcatgta
      721 ggccgcaacg tccttgtcga tcttgcccgt cagccggtcc cagagcggcc gcggatagcc
      781 gtcctttccg acggggccgt aggcggcgtc ccacgcggcg atttgtccgc cggaacggac
      841 gttggacccc agcaccgctt cggcctggct catctcgcgc atcgtctgcg tcacctggcc
      901 gtcgatcgta cgggagagcg gccgctccag ccgggcccaa tcgccgcgcg gcacggaaaa
      961 cgcattcgca tcttcataga cgttgccgac ctggtagcgg cgaaaatcga ccggatcggg
     1021 atagagcgac cagacgccgc taaagaactt gggatgaaag atctgcaggg cgagcgcttc
     1081 ccagccgccg gtcgagccac ccgtgagcac gcgggcctcg ggctgcggca gcagccggta
     1141 cttttgctcc aggtagggaa tcagctcttg cgtcagggcg tcgccgtacg gaccgttgtt
     1201 cgccgagttg accgcgtagg aatcgtcgta atagggcgtg ggatgctgaa aggtcacggc
     1261 gaccatccgg ggaaaatcgt cggaggtcca ggcccggtaa aactcgggtc cgcggccgaa
     1321 tgccatcccc gggggcgcaa aacccaacgg cggccgcagg ttgaagtgcc cctgcacata
     1381 gatggccgga tacttttgct cggtgtcctg gttgtatccg cgcggcaaga gtacggtcgc
     1441 accgaggtag atcgggcagc cccagaactc cgagagcagg tggctctgaa tcttgacccg
     1501 cttgacccat tcggtgtcgg gcggaacctc gacagccggc agcttcttgt cgagcgcgag
     1561 cctcaccgcg aaacccgcgg ccggatcgag gcggacgcgc tggacgctgc tgacgagatt
     1621 gccgggcgag cggttccact gctgcccctc ccactgatcc aggtggaccc agaccgtgtg
     1681 gccgtccttg cggcggacct gcgtatagac gttcagcacg gcctgcaccg agtagtcgcc
     1741 tgccggcagg tccttcaggc tggaaaccgg atatccgagc gcccgttcat cgatcgtggc
     1801 gctctggccc ggcgcgaggc cggcgacgtc cacgccaaaa aacggcgtcg atcccgcaaa
     1861 gctgctggcg agcagccgcg gctcgcggct gccttccttg gcgataatca ccaggagtcg
     1921 gccggtgagc ggccccggat cgacggacgc atcaaagctg acgtcgaatc gtaattcagc
     1981 cagcgcaggc cgggcgcaga caaggaccag caggcagacc gcgcgatgca gagcactggt
     2041 cacggaactg tctttcatgg cgtgcccttg atccgcttcg ccttggtctc gcgctcgccc
     2101 tgcttcagga tcaggtgcgt gacctggccc tcgtcgtcct tgacgaaggt gatctcggcg
     2161 ctgacttcct tgacgaagaa ctgggcgtcc gactggggat gcagctcggc cttgggttgc
     2221 ccagtgggtt gagccaggag tcggtcgccg tcgcgcgtga cggcgagcac gagattgggg
     2281 ccgatctcgt attcgcccgt gtatgcgtcc agaatcttgg cgtcgagttt cacgaacttg
     2341 cgctccttcg gcaattcgta gggctcgccg agggcgatcg ccgccaggcc gctcgcgacc
     2401 attgcggccg gcgtgccttc aatgttgctg agcacgacca cgcacagctt ctcgtcgggg
     2461 tagcgggcga tgaaggtcga gaagccgtta atcccgccgc cgtgctgcat ccgctggcgg
     2521 ccgaattgct tgtcgatgat ccagccgtag ccgtagttgc tcttgcccgg cgtgaacatc
     2581 ttttcgtagg ccccgctgga gatgagctgg ctgctagcca gcgcctgact ccagcggtgc
     2641 aggtcggtgg cggtggaata gagcgccccg gcgccatgcg gaatcgtcat atccaagtaa
     2701 ggggcattgg ccacgccgtc cccctcgcgc cgatagccgg cggcccgatg cgggagaatt
     2761 ttctccggcg agtcgtagcc ggtgtcggcc aggcccagcg gctgaaagat gctttcgcgg
     2821 aggaattcgc cataggactt gcccgagacc ttttcgatga tctgcccgag cagcacgtaa
     2881 cccgaattgc tgtagttgaa tttctcgccc ggcgtaaact ccagcggcag ttcgcggacg
     2941 cggtcgagcg tcttggccgc gggcgaaggc agcggcatcg ccgcacggta acccggcagg
     3001 cctgtgaagc tcggaatgcc gctggtgtgc gtcagcaggt ggtggatcgt tacttccttc
     3061 caggcttcgg gggtgccttc gacgtgcttg gatgccagat cctccaccga cagcttccct
     3121 tgctcctgaa gtatgaggat cgccatcgcg gtgaactgct tggtgatcga gcccagccga
     3181 aatttggttt ggggcgtgtt ggggatttcg tgctcgacat tcgccagacc cagtcctttg
     3241 gccagcagca cctgctcgcc tttggcgacg agcaccgagc cgctgaaacg gttcaccgcg
     3301 acctgcgcct ggagaaactc gttcgcctgg gcggcgacgg tttccgcgga aggcggctgc
     3361 tttggttcct gggccggcgc tggcagtgcc cagaccccaa taaagacgat cgcgacgatc
     3421 tgagatgcgg tgcgcaagcg gtaatgccga gtcatgagcg gtcccgctga gatccgacag
     3481 gaaagttccg aatagcggac ccgattgtac atgcccgccg attgctccgc attgcgacta
     3541 tagagagggg attgcgaatc gcactgcgcg ggcgtggagg cag
//
LOCUS       DASRJB010000297         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_367251, whole genome shotgun sequence.
ACCESSION   DASRJB010000297 DASRJB010000000
VERSION     DASRJB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367251"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1216
                     /gene="prfB"
                     /locus_tag="VFB80_08010"
     CDS             join(88..156,158..1216)
                     /gene="prfB"
                     /locus_tag="VFB80_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150074165.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZN33748.1"
                     /translation="METEWYNRAEAIRSRITQLRDSLDFEGKRKKVAEIEGRMAAEGF
                     WSNQEKAREAVAELKGLKAVLKPLGDLVRTGDDLPGMLELAEADESFAAEIRGEIQRL
                     EQLADELELKALLSGPHDALGAIVSINARDGGTDANDWAEMLLRMYTAWAQKNDYDVV
                     LLDRQDNDIAGIQSATITIRGPMAYGYLKGETGIHRLVRISPFNAEGKRQTSFAAVDV
                     SPEVSDEKEIEVDWAKDVREETMRAGGAGGQHVNKTESAIRLVHYETGIEVKCQNERS
                     QHQNRAMARKMLIARLVRLADEKREAEQYAKYKNVAKVGFGSQIRNYFLHPSQRVKDS
                     RTGYLEHHFHDVLDGNLQGFLDSYLKWRVKGELAGSLGDEE"
     gene            1244..1756
                     /locus_tag="VFB80_08015"
     CDS             1244..1756
                     /locus_tag="VFB80_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33749.1"
                     /translation="MRSEALEAVGPGGALLRVEFVWRGDRYGHVIAAADAAGQLQPLL
                     ESLEGTPTDDFPPSPPLQNLHRETLPDGRAALLLVGAAGRSHWSASIEAASGAPQLIF
                     DIACRRGGEGGWLGCRYLAMGGAKSPLSLESDDAVVTWREGEVDIRPATNRSATTRWR
                     FTVSLVTPDS"
     gene            complement(1780..>2599)
                     /locus_tag="VFB80_08020"
     CDS             complement(1780..>2599)
                     /locus_tag="VFB80_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33750.1"
                     /translation="LARRAFVAGSLAGLSAALLPLRPAAAAAPGEADTSQQARDEAVR
                     SLPLGELTAETRRKLMAVIERPSIYRRLPQKSIDCDPALFLFLIRNPEVVVNIWELMG
                     MSTNMTAQRQGPYLWKGNDGSGTVCDVELVYGTDDLHVMYCDGFYEGSLLKSKHTGRC
                     VLVLKSGYGQGDDRRWYVGNRLDVFVQIDNVGADFIARTLSPWIGKQADHNFQESCKF
                     ATKLSQTAETNAAGVQRLADKLTRVDPGVREQFARLAAGVQQRAAQRDVGVQRR"
BASE COUNT          480 a          858 c          878 g          383 t
ORIGIN      
        1 tgaaaggtcc gccgcctcca ccgccgtagt agttcctcgc gattccagcg gagaacgtcg
       61 cgacgctcca caaactagat ttgtctcatg gaaaccgaat ggtacaaccg ggcggaggcg
      121 attcggtcgc ggatcacgca attgcgagac tcgctttgac ttcgagggca agcggaagaa
      181 ggtcgccgag atcgaaggcc ggatggcggc cgaggggttc tggagcaatc aggaaaaggc
      241 ccgcgaagcg gtcgcggagc tcaaggggct aaaggccgtt ctgaagccgc tgggcgactt
      301 agtgcgcacc ggcgacgacc tgcccggaat gctggagctg gccgaggccg acgagtcgtt
      361 tgcggccgag atccgcggcg aaatccagcg cctggaacag ctggccgacg agctggagct
      421 gaaggccctg ctcagcggcc cacacgatgc gctgggcgcg atcgtcagca tcaacgcccg
      481 cgacggcggc accgacgcca acgactgggc cgagatgctg ctgcggatgt acaccgcctg
      541 ggcccagaag aacgactacg acgtcgtgct cctggaccga caggacaacg acatcgccgg
      601 catccagagc gccaccatca ccatccgcgg gccgatggcc tacggctacc tcaagggcga
      661 gacgggcatc caccgcctcg tccgcatcag ccccttcaac gcggaaggca agcgccagac
      721 cagcttcgcc gccgtcgatg tctcgcccga agtcagcgac gagaaggaaa tcgaagtcga
      781 ttgggccaag gacgtccgcg aggagacgat gcgggccggc ggcgcgggcg gccagcacgt
      841 caacaagacc gaaagcgcga tccgcctcgt gcattacgag accggcatcg aggtcaagtg
      901 ccagaacgag cggagccagc accagaaccg ggccatggcc cgcaagatgc tcatcgcccg
      961 cctggtccgc ctggccgacg aaaaacgcga ggccgagcaa tacgctaagt acaaaaacgt
     1021 ggccaaggtc ggcttcggct cgcagatccg caattacttc ctgcatcctt cgcaacgcgt
     1081 caaggacagc cgcaccggct acctggagca ccacttccac gacgtactgg acgggaattt
     1141 gcaggggttt ctcgattcct acttgaagtg gcgcgtcaag ggcgagctgg cgggcagcct
     1201 gggggacgag gagtaggggc gaatgcggaa tgcggagtgc ggaatgcgga gtgaagcgct
     1261 ggaggccgtg ggaccgggcg gcgcgttact gcgagtcgaa ttcgtctggc gcggcgaccg
     1321 ctatggccat gtgattgccg ctgccgatgc cgccggccag ttgcagccgc tgctcgaatc
     1381 gctggaaggg acgcccaccg acgattttcc gccgagtccg ccgctgcaga atctgcatcg
     1441 cgaaacgctg ccggatggcc gcgccgccct gctgctcgtc ggcgccgcag gccgcagcca
     1501 ctggtcggcc agcattgaag cagccagcgg cgcgccgcag ctgatcttcg acatcgcctg
     1561 ccgccgcggc ggcgagggtg gctggctggg ctgtcgctac ctggcgatgg gcggcgcgaa
     1621 gtcgccgctg tcccttgagt cggacgacgc cgtggtgacc tggcgggaag gggaagtcga
     1681 catccggccc gcaacgaacc ggtcagcgac gaccagatgg agattcacgg tcagcttggt
     1741 gacgcccgac tcctgacccc cgactcctga cccccgatcc taccgccgct gcacacccac
     1801 gtcgcgctgc gcggcccgct gctgcacgcc ggcggccagg cgggcgaact gttcccgcac
     1861 gccgggatcg acgcgggtca gcttgtctgc cagccgctgc acgcccgccg cgttggtctc
     1921 ggccgtttgt gagagcttgg tcgcgaactt gcacgactcc tggaagttgt ggtcggcctg
     1981 cttgccgatc cagggcgaga gcgtgcgggc gatgaagtcg gcccccacgt tatcgatctg
     2041 cacgaacacg tccaggcggt tgcccacata ccaccgccgg tcgtcgccct ggccgtagcc
     2101 cgacttcagg accagcacgc agcggccggt gtgcttgctc ttgagcagcg agccttcgta
     2161 gaagccgtcg cagtacatca cgtgcaggtc gtcagtgccg taaaccagct ccacgtcgca
     2221 gactgtgccg gagccgtcgt tgcccttcca cagatacggc ccttgccgct gggccgtcat
     2281 gttggtcgac atacccatca gctcccagat gttgaccacg acctccgggt tgcggatcag
     2341 gaacagaaac agcgcggggt cgcagtcgat cgatttctgc ggcagacggc ggtagatgct
     2401 ggggcgctcg ataaccgcca tcagcttgcg gcgggtttcg gcggtgagct cgcccagcgg
     2461 cagcgagcgg acggcctcgt cgcgggcctg ctgcgaggta tcggcctcgc cgggcgcggc
     2521 cgcggcggca ggccgcagcg gcagcagcgc cgccgacaag ccggccagag agcccgcgac
     2581 aaacgcccgc cgcgcgagc
//
LOCUS       DASRJB010000298         6430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3467818, whole genome shotgun sequence.
ACCESSION   DASRJB010000298 DASRJB010000000
VERSION     DASRJB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467818"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..3688)
                     /locus_tag="VFB80_08025"
     CDS             complement(254..3688)
                     /locus_tag="VFB80_08025"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN33751.1"
                     /translation="MEKPTFFARRALLILVVVFFFVPFALRGARMALLGMKNDVKDWL
                     PKDFEETRDLDEFRRYFLSEQFVLVSWDGCSGDETDERFRLFLAKLNPELPPSVLAAE
                     AKAAEEKAAAAGEGAAGPEAEPQRSNPTVALHRDENFIGDKLGLYYGGDWYENWGARG
                     EKWLKGLRLAKEGSNEECWYFLTPDGDLFRWDEVDAPPAALARAVYRRFVDKRTEGTL
                     VHSFGLKDGAWYHADPRRLRAQLFKTVTTGPAVLESLTREGGELSGSEELAAEAKRRL
                     SGVLFGEGGKTTCMMLTLTDAARRNLHLVCGRGLLGKPRGRLFEIAHESNISESELRL
                     GGPPVDNVAIDEEGSVTLVRLVSWSAVLGIGLSLICFRSISLTIMIFFVGGISAVMSL
                     AAVWWLGSTADAITLSMPSMVYVLGLSGAAHIINYYRDAVDHHGLAGAPERAIAQGWK
                     PAFLCEVTTAIGLITLVTSELVPIRKFGLFSGLAVMGMFLVMMTYLPAALQIWPQPPR
                     KKRASSEPSWLAGALDSFWPKFSGWIVNHHALVAVGCTLVIAAFGYGVVYMRTSVNLL
                     KMFHSEAKIIRDYQWLEANLGPLVPMEIVVRVPAASQRPSIARRRELEDELAQAETPA
                     ARKTEIERQLRETPFQLSFLERMELATRVQRQIEAEFGPSGRGIVGRSVCASTFVRPL
                     PEPSGSASAQTTRATMSSLLEKHKAEFLHSDYLRVKQEDQTEMWRVSLRVGATNGVDY
                     GAFVSDLQQTVEPIVAAEQQRSAILRQLHESLQRRGDADPALAGSTVVLVGVPEAALA
                     RRQAGAAPPAPTAVDQQKIFARTLYELLVNSRMRLTTLAAGKSLPADLNETLAASDCV
                     VVVGDMPGLDLARLRGSAPLVIDARVQPLVPGQRTAWQRDPASVAAVYTGVVPIVYKA
                     QRLLLDSLIQSTFWSIITITPLLMWIARSFSGGAVSMLPNVLPIVMVFGGMGWLGIDV
                     DVGSMMTASIALGVAVDDTIHFLNWFRVELDRLGDRKQAILATYKHCATPTLQAAVIS
                     GLGLSIFALSTFTPTQRFGCLMLVILWAGAIAELIYFPAILAGPLGRVFQPRKQPATE
                     PQPAAEDKSQPQFQVVHHDDEPEESLAAHSGNGHPPPPHTGKAPHRQIRADAAHRR"
     gene            4189..4509
                     /locus_tag="VFB80_08030"
     CDS             4189..4509
                     /locus_tag="VFB80_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33752.1"
                     /translation="MLASFVLSVAMSTTMVPPGEEVVNHAHPGHYAQPTIRIFERRGE
                     DTDRKLAWRGYCQELDQLWFEYRAAGSTPAAWRKYVAEAGQAKRRYVFRDPYLAPIVD
                     DFAW"
     gene            complement(4540..5331)
                     /locus_tag="VFB80_08035"
     CDS             complement(4540..5331)
                     /locus_tag="VFB80_08035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN33753.1"
                     /translation="MVFPVADDNSDRTIFPWLNIGLIAVNVLVFVVFQGMGGNDRFTY
                     AFSTVPAEITTGRDLVTEPHVEQVETPVGVQRIKVPGLEPTPLSVYLTLLTSMFMHGG
                     IAHLLGNMWFLWIFGDNIEQDMGRVRYLVFYLVCGLLASLTHVVLNAGSQIPSLGASG
                     AISGVLGAYLLLHSQRRVTVVMFRFLTQVPGWVAVGLWFAFQVIQGLGVLGGEGTGVA
                     YGAHIGGFIAGLALAKVFMIGRPTTTEQKASYGQQRYRKNELERW"
     gene            5626..>6430
                     /locus_tag="VFB80_08040"
     CDS             5626..>6430
                     /locus_tag="VFB80_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33754.1"
                     /translation="MTQCLSEADILGYIQGSVDDQQRSAFDEHVSSCPNCQARVEIAR
                     ESSLDVRSPATHQAITQVEATSAHEIEPAKAAGSHWTDGDVASQVVIQVPQTAPKVSL
                     DEFLTGLSQSGLMPAAEVASVRDRSHKDPATSTVAGLIEWLVQEHKLTRYQANLLARG
                     HRHGLVLGNYVIQDKLGQGGMGAVFKARHRRMNRLVALKVLPQSLSSIPEAIARFQRE
                     VEAAARLTHPNIAAAYDADEAGGVHFLVMEYVEGPTLANYVKHRGSLPVA"
BASE COUNT         1134 a         2083 c         2126 g         1087 t
ORIGIN      
        1 gagcagcaac gacgacgatc ggctgcacga ccgttcccag cagccgcacg acccgctcga
       61 gcatcaactc cggtccgaac ggcagcgtgg ccttgggcag tcccatccgc gagctgcgtc
      121 cgccggcgag gacgatgccg gagcaggcga tttcagattt caaatttcag atttcaaatt
      181 tgggaatagg cgctcaattt gggtttattt tcctctgcaa atctgaaatc tgaaatccta
      241 aatctgaaat tccctatcgc cggtgcgccg cgtcggcgcg gatctggcga tggggagcct
      301 tgccggtgtg gggcggcggc ggatggccgt tgcccgaatg ggccgcgagc gattcttccg
      361 gctcgtcgtc gtggtggacg acctggaatt gcggctgcga tttgtcttcg gctgccggtt
      421 gcggctcagt cgcgggctgc ttccgcggct gaaagacccg gccgagcggg ccggccagga
      481 tcgcggggaa gtagatcagc tcggcgatcg ctccggccca cagaatcacc agcatcaggc
      541 agccgaagcg ctgcgtgggg gtgaacgtgc tgagggcgaa gatcgacaaa cccaggccgc
      601 tgatgacggc cgcctgcagc gtcggcgtgg cgcagtgctt gtaggtcgcg aggatcgcct
      661 gcttgcggtc ccccagccgg tcgagctcga cgcggaacca gttgaggaag tggatcgtgt
      721 catcgaccgc gacgcccagg gcgatgctgg ccgtcatcat cgagccgaca tcgacgtcga
      781 ttcccagcca gcccatgccg ccgaagacca tcacgatcgg cagcacgttg ggcagcatcg
      841 agaccgctcc gccgctgaag ctgcgggcga tccacatcag cagcggcgtg atcgtgatga
      901 tcgaccagaa cgtgctctgg atcaggctgt cgagcagcag ccgctgggcc ttgtaaacga
      961 tcggcacgac gccggtgtag acggcggcga cgctggcggg atcgcgctgc caggcggtcc
     1021 tctgccctgg gaccagcggc tgcacgcggg cgtcgatcac tagcggagcc gagccgcgga
     1081 ggcgggccag atcgaggccc ggcatgtccc ccacgacgac gacgcagtcg ctggcggcga
     1141 gcgtctcgtt caggtccgcc ggcagcgatt tgccggcggc gagtgtggtc agtcgcatcc
     1201 gcgagttgac gagcagctcg tagagcgtgc gggcgaaaat cttctgctga tcgacggctg
     1261 tgggggcggg cggggcagcg ccggcctgcc gccgggcgag ggccgcctcg ggcacgccga
     1321 ccagcacgac tgtggagccg gccagggcgg gatcggcgtc tccgcgacgc tgcagcgact
     1381 cgtgcagctg gcggaggatc gccgagcgct gctgctcggc ggcgacgatc ggctcgacgg
     1441 tctgctgcaa gtcgctgacg aacgcgccgt aatcgacgcc attggtcgcc ccgacgcgga
     1501 ggctgacccg ccacatctcg gtctggtctt cctgtttgac gcggaggtaa tcgctgtgca
     1561 ggaactcggc cttgtgcttc tccagcaggc tgctcatcgt tgctcgcgtc gtctgtgcgg
     1621 aggcgctgcc gctgggctcg ggcagcggcc ggacaaacgt ggaggcacaa accgaccggc
     1681 cgacgatgcc gcggcccgac gggccgaatt cagcctcgat ctgccgctgg acgcgcgtcg
     1741 ccagctccat ccgctcgagg aacgagagct ggaacggcgt ctcgcgcagc tgccgctcga
     1801 tttccgtttt gcgggccgcg ggcgtctcgg cctgcgccag ctcgtcttcg agctcgcggc
     1861 ggcgggcaat gctgggccgc tggcttgcgg ccggcacgcg gacgacgatc tccatcggca
     1921 cgagcgggcc caagttggcc tccagccact ggtagtcgcg gatgatcttg gcctcgctgt
     1981 ggaacatctt cagcaggttc acgctggtcc gcatgtaaac gacgccgtag ccgaaggccg
     2041 cgatgacgag cgtgcagccg acggccacca gcgcgtggtg gttgacgatc cagccgctga
     2101 acttgggcca gaagctgtcc agggcgccgg cgagccagga aggttcgctg ctggcccgct
     2161 tctttcgcgg cggctgcggc cagatttgca gggccgcggg caggtaggtc atcatcacca
     2221 ggaacattcc catcacggcc aggcccgaga acaggccgaa cttgcggatc gggacgagct
     2281 cgctggtgac cagcgtgatc aggccgatcg ccgtcgtcac ttcgcacaag aaggccggct
     2341 tccagccctg agcgatggcc cgctccggcg cgccggccag cccatgatga tcgaccgcgt
     2401 cgcggtaata gttgatgatg tgcgccgcgc ccgacagccc gaggacatag accatcgagg
     2461 gcatcgagag cgtgatcgcg tcggccgtgc tgcccagcca ccaaaccgcg gccaggctca
     2521 tgaccgccga gatgccgccg acgaagaaga tcatgatcgt cagcgagatg ctgcggaagc
     2581 agatcagcga caggccgatc cccagcaccg ccgaccagct gaccagccgc acgagcgtga
     2641 ccgagccttc ttcgtcgatg gcgacgttat ccaccggcgg gccgccgagc cgcagctcgc
     2701 tttccgagat gttcgattcg tgggcgatct cgaacaggcg gccgcgcggc ttgccgagca
     2761 ggcctcggcc gcagaccagg tgcaggttgc ggcgggcggc gtcggtcagc gtcaacatca
     2821 tgcaggttgt cttgccgccc tcgccgaaca gtacgcccga cagccggcgc ttcgcctcgg
     2881 ccgccagttc ttcgctgcct gacaattcac ccccttcgcg ggtgaggctc tctagtaccg
     2941 cggggcccgt cgtgaccgtc ttgaacagct gggcccgcag ccggcgagga tcggcgtgat
     3001 accatgcgcc gtccttaagc ccgaaggagt gaaccagcgt cccctcggtg cgcttatcaa
     3061 cgaatcgccg atagacggca cgggccagcg cggccggcgg tgcatcgact tcgtcccagc
     3121 gaaacaaatc gccgtcggga gtcaggaaat accagcactc ttcgttgctg ccctccttgg
     3181 cgagtcgcag gcccttgagc cacttttcgc cccgagcgcc ccagttttcg taccagtcgc
     3241 cgccgtagta gagccctagc ttgtcgccga tgaagttctc gtcgcggtgg agcgctaccg
     3301 tgggattgct gcgctggggt tcggcctcgg gccctgcggc cccttcgccg gcagcggctg
     3361 ctttctcttc cgccgccttg gcttcggccg ccaggaccga tggcggcaac tcggggttca
     3421 gcttggccag gaacaggcgg aaccgctcgt ccgtctcgtc gccgctgcag ccgtcccagc
     3481 tcaccagcac gaactgttcg ctaaggaagt agcggcggaa ctcgtcgagg tcgcgggttt
     3541 cctcaaaatc cttgggcagc cagtccttga cgtcgttctt catgcccagc agcgccatcc
     3601 gagcgccgcg cagggcaaag ggcacgaaga agaagaccac caccagaatc aggagcgcgc
     3661 ggcgggcgaa gaaagtcggc ttttccatgt tggctcgtgg acggaagctc tcgcccggga
     3721 ggtcgagctg cggctagcgc ccagcctcag gtggggcggc ggctggcgga ggcggggttg
     3781 gccgcgggca ggtgcgaatt gacaggcgct accttgaact tcgacaaaat gggaaagccg
     3841 gcttgaaatg acaaagccgc gagcaagcta tttgctggtg ctagcggtat tcgcggaatg
     3901 tccctcggtg tcgctctgcg ggttgatttg ggaaaacgtc ctcgcaacat atcccccggc
     3961 gcgaggcata tcaagggttg ccatgatttg ctcaattgtt tcgcaagaat tacgaaatct
     4021 gctctaagtt gttgagagca ctccacttat cgacgagata gcggacttgg cgcgggagat
     4081 tgtgcggcct gcggaaactt gacgggctga tcggctgcaa gcacgattga acgtaagcta
     4141 gagtcgcttg ctgcccaatc tctgtccggc cccacgagga gttccgcaat gcttgcgtcg
     4201 tttgtgctgt ccgtcgcgat gtccaccacg atggtcccgc cgggcgagga ggtcgtgaat
     4261 cacgcccacc ccggccacta tgcccagccg acgattcgga tctttgagcg gcgcggcgag
     4321 gacaccgacc ggaaactggc ctggcggggc tattgccagg aactggacca gttgtggttc
     4381 gagtatcggg ccgccggctc gacgcccgcc gcctggcgga agtacgtcgc cgaagccggc
     4441 caggccaagc ggcgctacgt cttccgcgat ccctatctgg ctcccatcgt ggacgacttc
     4501 gcctggtaag cagtcttcac gctccgcgta aagcaggcgt caccagcgtt ccagttcgtt
     4561 cttgcggtag cgctgctgcc catagctggc cttttgctcg gtcgtggttg gtcggccgat
     4621 catgaagacc ttcgccaatg ccaggccggc gatgaagccg ccgatatgcg ccccgtaggc
     4681 gacgccggtc ccctcgccgc ccagcactcc cagcccctgg atcacctgga aggcgaacca
     4741 caggcccacg gccacccagc cgggaacctg ggtcagaaag cggaacatca cgacggtcac
     4801 gcggcgctgc gaatggagca ggaggtaggc cccgaggaca cccgagatgg cccccgaagc
     4861 gccgaggctg ggaatctgcg acccggcgtt gagcacgaca tgcgtgagcg aagccagcag
     4921 cccacagacg aggtagaaaa ccaaatacct gacgcggccc atgtcctgct cgatgttgtc
     4981 gccaaagatc cagagaaacc acatgttccc cagcaggtgg gcgatcccgc cgtgcatgaa
     5041 catggaggtc agcagcgtca gatagacgga gaggggcgtc ggctccaggc cggggacttt
     5101 gatccgctgc acgcccacgg gcgtctcgac ctgttcgaca tggggctcgg tcaccaggtc
     5161 ccggccggtg gtgatctcgg ccggcaccgt cgaaaaggca taggtaaagc ggtcgttgcc
     5221 gcccatgccc tggaagacca cgaacaccag aacgttgacc gcgatcaggc cgatgttgag
     5281 ccagggaaag atcgtccggt cgctgttgtc gtctgctacg ggaaagacca tgccagcgaa
     5341 ctccgttgcc gtacctttat gctcccgccg cctgccgccc tgtctggagc cctcaatgtc
     5401 acaccgggcc ggccaacccg caattggggt gccggggatt tcggtatggc gtgctgccgc
     5461 ttagtaactc gccctaagcg attgcctcgc aaccagttgc gtcgcgtcca acgggcagcc
     5521 acaatgccgc ttcgccgcct caaccaccca ctagctgtac ggccgccagc ggcgctttag
     5581 aatggacggc tcatcttgcg ggtggttggc tcgaggtacg cccgaatgac gcaatgcctg
     5641 agcgaagccg acatcttggg ttacatccag ggttccgtag atgaccagca acgatcggca
     5701 tttgacgaac acgtaagcag ctgccccaac tgccaggccc gggtcgaaat cgcgcgggaa
     5761 tcgtcactcg acgtgcggtc acccgcaacg caccaggcga tcacccaggt cgaggcgacc
     5821 tcggcccacg agattgagcc tgcaaaagcc gccggttccc actggaccga cggcgatgtt
     5881 gcctcgcagg tcgtgattca ggtgccccag acggccccca aggtctcgct cgacgaattc
     5941 cttaccggac tttcgcagag cggcctgatg ccggctgccg aggtggcgag cgtcagggac
     6001 cgttcccaca aggatcctgc gaccagcacc gtcgccggcc tgatcgagtg gctggtccag
     6061 gagcacaagc tcacgcggta ccaggcgaac ctgctggccc gcggccaccg ccacggcctg
     6121 gtgctcggga actacgtgat ccaggacaag ctgggccagg ggggcatggg ggccgtcttc
     6181 aaggcccgtc accgccggat gaaccggctg gtggcgctga aggtgttgcc tcagtcgctg
     6241 tcgagcatcc ccgaggcaat cgcccgcttc cagcgcgagg tcgaagcggc cgcccgcctg
     6301 acgcacccga atatcgccgc ggcctacgac gccgacgaag cgggcggcgt gcactttctc
     6361 gtgatggagt atgtcgaggg cccgacgctg gccaattacg tgaagcaccg cggaagcttg
     6421 ccggtggcga
//
LOCUS       DASRJB010000299         7819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4033962, whole genome shotgun sequence.
ACCESSION   DASRJB010000299 DASRJB010000000
VERSION     DASRJB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033962"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFB80_08045"
     CDS             complement(<1..441)
                     /locus_tag="VFB80_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33755.1"
                     /translation="MSRYSREISVAAAYALLLLVLWIAAPSFYAGDKLRAILVASAPV
                     LVAAAGMTLVILTRQIDISIGSQFSVCGIAAGLLAAAGLPMPLVAVLTVGLGGLLGAI
                     NGGFVAALSLPSIVVTLATMVVLREGLRWAREGEFVKNLPPGFQW"
     gene            complement(438..1949)
                     /locus_tag="VFB80_08050"
     CDS             complement(438..1949)
                     /locus_tag="VFB80_08050"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZN33756.1"
                     /translation="MTTILRATTISKQFAGVRALREVSFEVRAGEVHALVGENGAGKS
                     TLIKIITGAVQPDAGTLEFAGRPVEHNDPVAARRLGIAAIYQQPALFPDLTVAENIAI
                     GLESGGPWRRVRWRERQSRARELLARVGASISPGRIVDQLSMPEQQLVEIARALGADA
                     RLLILDEPTASLSEREAQRLLQVVRDLRSHGVGLVYISHRLDELFQVADRVTVLRDGT
                     AVATRPMNEVDRAELIRLMVGRELTAVFPKQTVPLGETVLELAGIGCRAAGVRDVNLK
                     IRAGEILGLAGLVGAGRTELARILFGLTPADAGQILLRGRRVTIDSPARAVELGLACV
                     PEDRRRHGVILDLPIAVNTSLAILQQISARGRLDFRRERSLARSMVERLAIKTPSIDS
                     PAVELSGGNQQKVAIARWLATEPAVLILDEPTQGIDIGAKAEIHRLMGELASRGMAIL
                     MISSELPEILGMSDRICVMRGGRIVGALERDGATQERVLALALGHAQSERRAG"
     gene            complement(1946..3208)
                     /locus_tag="VFB80_08055"
     CDS             complement(1946..3208)
                     /locus_tag="VFB80_08055"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HZN33757.1"
                     /translation="MNAERAFALAREQYAELGVDAAAALTALQGVAVSLHCWQGDDVG
                     GFESAAGLTGGGIQATGNYPGKARTPDELRADLDAALALIPGRHRVNLHAIYADYAGR
                     QIGRDELLPEHFAAWIEWAKSRRLGLDFNGTFFSHPLAESGFTLASRDEGVRRFWVRH
                     ALACREIGEVFGRELGTPCVTNVWIPDGYKDTPVDRQTPREILRRSLDEIFAVPLDPR
                     HHLDAVESKLFGIGSESYVVGSHEFYLGYAIAKGKLLCLDAGHFHPTEGIADKISSIL
                     LWLPEILLHVSRGVRWDSDHVVVLSDDLRQIAEEIVRGGHLKRVHIGLDFFDASINRI
                     AAWVIGTRALLKGLLLALLEPTAMLRQCENEGDYTQRLALSEELKSLPAGAVWDYYCE
                     TQNVPVGLTWLDHARAYERDVLSQRGCP"
     gene            complement(3287..3691)
                     /locus_tag="VFB80_08060"
     CDS             complement(3287..3691)
                     /locus_tag="VFB80_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33758.1"
                     /translation="MTDLDRHQLRIVVADDNQDAALTLSMLLDRLGFDVIAAVFDGES
                     ALECIRTERPHVAVLDIAMQGLDGLEVAQQARADAGAALRLVAVTGLGSACDRADALR
                     AGFDAYFSKPVEGQKLEELMLAYLQSGIARPG"
     gene            4080..4973
                     /locus_tag="VFB80_08065"
     CDS             4080..4973
                     /locus_tag="VFB80_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33759.1"
                     /translation="MKSLVQWLAVLAAAGILLPASGACAEPTVTRDLEYARAGETSLQ
                     LDLYVPPEAATPRLIVWVHGGAWRSGSKKDVPILPLADRGFAVASVDYRLSPVAPYPA
                     QVHDLKAAIRYLRGTAGKTGIDPRHIAIAGGSAGGHLAALVGVTNGSSAHEGDLGEHR
                     GVSSNVQAIIDFFGPTNLTTILAQSTPHGLSVRAPALELLFRGKPEDKIELARLASPV
                     LQVDRSEPPLLIFHGDQDPQVPINQAHELVGAYKKQGLAATFEVVYGGAHGGKLFYTP
                     EQLDQVAGFLDAHLQPAKSQP"
     gene            4977..5978
                     /gene="pdxA"
                     /locus_tag="VFB80_08070"
     CDS             4977..5978
                     /gene="pdxA"
                     /locus_tag="VFB80_08070"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZN33760.1"
                     /translation="MSDLPRIAVTMGDPAGIGPEVCLHLLADAGIARRCAPVVFGDAE
                     ILRRVSAAARLPLNSPVITPQEWQANCTQIAAPTIVDWRAVDAERVQPGVVSAATGRA
                     GFTYVERAIDAALAGQVAAVSTAPLNKEALNAAGVRYPGHTEIFADRTKAPRSCMMQY
                     SEEITCTFVTVHVGYRDVPGLLTKERILDVIELTADALARLRSKRPKIVVCGLNPHAG
                     EHGLFGDREEERIIVPAIEAARAQGIEIEGPLPPDTAFLPAKRKATDAFVCMYHDQGH
                     IPVKALAFDSAVNTTLGLPIVRTSVDHGTACDIAWQGKANPSSIFSAVQLALRLIAA"
     gene            5983..7059
                     /locus_tag="VFB80_08075"
     CDS             5983..7059
                     /locus_tag="VFB80_08075"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HZN33761.1"
                     /translation="MSIVILADDLSGAAELAGIAFARGLATEVQRTFEPASPAEVIAV
                     DTDSRHLPAADAAARVAAVARQIAASQPAWIFKKVDSVLRGNVRVEIEAILKATGQQQ
                     AVLVPANPSRGRTIARGQYLVDGLPLDQTQFAQDPEYPRRSAQIAHLLGGESAAIAVP
                     DVASLDDVGRCAQEQGDQSLAAGAVDFFAALLDRRVSRPAGSAGSAEFVLPRPALLVC
                     GSRLSWRRRSEECLAAGVSVQTLAADLPSAGSQLAATGRMLLGIGDIQSPLDSRELLS
                     HLAQLTAQVMNRTPLAALLAEGGATAAAIASVLGWQRFAVTAQAPSGVGILQPLTSAP
                     APLLLIKPGSYAWPEKIWRQFAAR"
     gene            7218..>7819
                     /locus_tag="VFB80_08080"
     CDS             7218..>7819
                     /locus_tag="VFB80_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HZN33762.1"
                     /translation="MASTKRTEGTSRTARKSGSLQTPLETYLREINETALLTAKDEQD
                     LAHLIALGDVRARDRMVRANLRLVVNIARGYTGKGLSLQDLIEEGNLGLLRAVEGFDP
                     AVGTRFSTYASYWIKQSIKRALINSAKTIRIPAYMVELLSKWRRASARLTEELGRTPT
                     PEEVGRILGLQKKKLPIIKKAIRIYNSTPQTDQAETGWSLG"
BASE COUNT         1316 a         2779 c         2523 g         1201 t
ORIGIN      
        1 ccactggaac ccgggcggca ggttcttgac gaactcgcct tcgcgagccc agcggagccc
       61 ttcgcgcagg acgaccatcg ttgccaacgt gacgacgatc gacggcagac taagagcggc
      121 gacgaagccg ccgttgatcg cgcccagcaa accgcctagg ccgaccgtca aaacggcgac
      181 gagcggcatc ggcaggccgg cggcagccag caatccggcg gcgatcccgc agaccgaaaa
      241 ctgcgagccg atcgaaatgt cgatttgccg cgtgaggatg accagcgtca tgccggcggc
      301 cgcgacgagc accggtgcgc tggccaccag gatcgcccgc agcttgtcgc cggcatagaa
      361 cgacggcgcg gcgatccaca gcaccagcag cagcagggca taggcggccg cgaccgaaat
      421 ctcgcgcgag tagcggctca tccggccctc cgctcgctct gggcatggcc gagcgccagc
      481 gccagcaccc gctcctgggt cgcgccgtcc cgctcgagcg caccgacgat ccgtccgccg
      541 cgcatcacgc aaatccggtc gctcatgccg aggatttccg gaagctcgga cgagatcatc
      601 agaatcgcca tgccgcggct ggccagctcg cccatcagcc ggtggatttc cgccttcgcg
      661 ccgatgtcga tgccctgggt cggctcgtcg agaatcaaca cggcgggctc cgtcgccagc
      721 cagcgggcga tcgcgacctt ttgctggttg ccgccggaga gctccaccgc gggcgagtcg
      781 atcgacggcg tcttgatcgc caggcgctcg accatggatc gcgccagcga gcgctcgcgc
      841 cggaaatcga gccgcccccg cgccgagatc tgctgcagga tcgccagcga agtattgacg
      901 gcaatcggca agtcgaggat cacaccatga cggcggcggt cctcggggac gcaggccagt
      961 cccagctcca cggcccgcgc gggcgaatcg atcgtgaccc gccggccgcg caacaggatc
     1021 tgccccgcgt cggccggcgt aagtccaaac agaatgcggg ccagctccgt ccgccccgcg
     1081 ccgacgagtc ccgccagtcc cagaatttcg cccgctcgaa tcttcaagtt gacatctcgc
     1141 acgccggcgg cgcggcaacc aataccggcg agctccagca ccgtttcgcc cagcggcaca
     1201 gtttgctttg ggaatacggc ggtcaattcg cggccgacca tcagccggat cagctcggcc
     1261 cgatcgacct cattcatcgg ccgcgtggcc acggccgtgc cgtcgcggag cacggtcacg
     1321 cggtcggcga cctgaaaaag ttcgtcgagg cggtgcgaaa tgtagaccag cccgacgccg
     1381 tgcgaccgca agtcgcgaac gacttgcagc agccgctggg cctcgcgctc gctcagcgaa
     1441 gctgtcggct cgtcgaggat cagcagccgt gcatcggcgc ccagcgcgcg ggcgatctcc
     1501 acgagctgct gctcgggcat cgacaactga tcgacaatcc ggccgggcga aatactggct
     1561 ccgacgcggg ccagcagctc gcgggcgcgg ctctgccgct ccctccagcg gacgcgccgc
     1621 caggggccgc cactctccaa gccgatggcg atgttctcgg ccaccgtcag gtcgggaaac
     1681 agcgccggct gctggtaaat ggccgcgatc cccagccgcc gcgcggcgac cggatcgttg
     1741 tgctcgactg gccgccccgc gaattcgagc gtgcccgcat ccggctgcac ggcgccagtg
     1801 atgatcttga tgagcgtcga tttgccagcg ccgttctcgc cgaccagggc gtgtacctcg
     1861 ccggcccgca cttcgaacga cacctcgcgc agcgcccgca cgccggcgaa ctgcttgctg
     1921 atggtcgtcg ctcgcagaat cgtggtcatg ggcaaccgcg ctgggagagc acatcccgct
     1981 cgtaggcccg cgcgtgatcg agccaggtca gtccgaccgg cacgttctgc gtctcgcagt
     2041 agtagtccca gacggcgccc gccggcagcg acttcagctc ttccgaaagc gccagccgct
     2101 gcgtgtagtc cccctcgttc tcgcactgcc ggagcatggc cgtcggctcc aacagcgcca
     2161 gcagcagccc cttgagcagg gcccgcgtgc cgatcaccca ggccgcgatg cggttgatgc
     2221 tggcgtcgaa aaagtcgagg ccgatgtgca cgcgtttcaa gtggccgccg cgaacgatct
     2281 cctcggcgat ttgccgcagg tcgtcggaga ggacgacgac gtggtcgctg tcccagcgga
     2341 cgccgcggct gacgtgcagc aggatctccg gcagccacag cagtatcgac gaaatcttgt
     2401 cggcaatccc ttcggtcggg tgaaagtggc cggcgtccag gcacagcagc ttgcccttcg
     2461 cgatggcgta tcccaggtaa aactcgtgcg agccgacgac gtagctctcc gagccgatcc
     2521 caaacagctt gctctccacg gcgtcgaggt gatgccgcgg atcgagcggg acggcgaaaa
     2581 tctcgtccag cgaccgccgc aagatctcgc ggggcgtctg gcgatcgacg ggcgtgtctt
     2641 tgtagccgtc gggaatccag acgttcgtga cgcagggcgt tcccagctcg cggccaaaca
     2701 cctcgccgat ctcgcggcag gcgagagcgt gccgcaccca aaaccgccgc acgccttcgt
     2761 cgcggctggc gagcgtgaag ccgctctcgg ccagcgggtg cgagaagaaa gtgccgttga
     2821 agtccaggcc cagccgccgc gacttcgccc actcgatcca cgccgcaaag tgctcgggca
     2881 acagctcgtc gcgcccgatc tggcggccgg catagtcggc gtaaatcgca tgcaggttga
     2941 cgcggtggcg gccggggatc agcgccaagg cagcgtccag gtcggcccgc aattcgtccg
     3001 gcgtccgcgc cttgccgggg tagttccccg tcgcctggat gccgccgccg gtcagtcccg
     3061 cggcactctc gaagccgccg acgtcgtctc cctgccagca gtgcagcgac accgcgacgc
     3121 cttgcagcgc cgtcagcgcc gcggccgcat cgacgcccaa ctcggcatat tgctcgcggg
     3181 caagggcaaa ggcgcgctcg gcgttcatcg cttcgggcca atcgcaggct gggacgagag
     3241 gtcaatccta agtcccagcc gcgcgatcct caatcgctcg ccgcgatcag cccgggcggg
     3301 caattcccga ctgcaaatag gccagcatca actcctcgag cttttgccct tccacgggct
     3361 tggaaaaata ggcatcgaag ccggcccgca gcgcatcggc ccggtcgcag gcgctcccca
     3421 gccctgtgac ggcgaccaat cgcagcgccg cgccggcgtc ggcgcgggcc tgctgggcga
     3481 cttccaaccc gtcgagcccc tgcatcgcga tatcgagcac ggcaacgtgc ggccgctcgg
     3541 tgcggatgca ctccagggcc gactcgccgt cgaacaccgc ggcgatcacg tcgaacccga
     3601 gacggtcgag cagcatcgac aacgtcaggg cggcatcctg gttgtcgtcg gcaacgacga
     3661 ttcgcagttg gtggcggtcg aggtccgtca atttgtcgct ctcgcagcgg cgcgcaccgc
     3721 cggcgctggt cggcttgcgg ccagtttgat cgttaggcgt ccaacaattc tgccaacatc
     3781 cgagtcaata ccgtctcaaa ctcccggacc tcggcctcct gccaaccggc gtcggcccgc
     3841 agtctggcgg catgactcga caggcaatcc aggggccgaa gcgactggta gcagtcttcc
     3901 aagcattcct ggatggctcc gttcatcttt gcgtgatcca tgcatgtcct gggggtggag
     3961 agaggaggtc attaacctgc atgccgcgtg ccaagtggat ttgccctcgc ctctgttgcg
     4021 gcgacccgca gcaaggcatg atgacgatcg ggcgccgccg cctctcgaaa ggctgagcca
     4081 tgaaatctct cgtccagtgg ttagccgttc tcgccgccgc ggggatcctg ctgccggcca
     4141 gcggagcatg cgccgagccg acagtcacgc gcgacctgga atatgcccgc gcgggcgaga
     4201 cctccctcca gctcgacctc tacgtgccgc cagaggcggc gacgccgcga ctgattgtct
     4261 gggtccacgg cggggcgtgg cggagcggct cgaagaagga cgtgccgatt ctgccgctag
     4321 ccgatcgcgg attcgccgtg gccagcgtcg attaccgcct ctcgccggtc gcgccctatc
     4381 cggctcaggt gcacgacctc aaggctgcga tccgctatct ccgcggcact gccggcaaga
     4441 ccggcatcga cccgcgccac atcgccatcg ccggcggctc ggccggcggg cacctggcgg
     4501 cgttggtcgg cgtgaccaac ggctcttctg ctcatgaagg cgacctcggc gagcaccgcg
     4561 gcgtttcgtc caacgtgcag gcgatcatcg atttcttcgg gccgacaaac ctcaccacga
     4621 tcctcgccca atcgacgccg cacggcttga gcgttcgcgc gccggccctc gagctgctct
     4681 tccgcggcaa gcccgaggac aaaatcgaac tggcccgcct ggccagcccc gtgctgcaag
     4741 tcgatcggag cgagccgccg ctgttgatct ttcacggcga ccaggatccg caggtgccga
     4801 tcaaccaggc ccacgagctg gtcggcgcgt acaagaaaca gggtctcgcg gcgacgttcg
     4861 aggtcgtcta tggcggcgct cacggcggca agttgttcta cacacccgag cagctcgacc
     4921 aggtcgccgg gtttctcgat gctcaccttc agcccgccaa gagccagcct taaccgatga
     4981 gcgacttgcc gcgcattgcc gtcacgatgg gtgaccccgc cggaatcgga ccggaggtct
     5041 gcctgcattt gctcgcggat gccggcatcg ccaggcgatg cgccccggtc gtcttcggcg
     5101 acgctgaaat cctgcggcgc gtcagcgccg cggccaggct gccgctgaac tcgccggtca
     5161 ttacaccgca agagtggcag gcgaattgta cacagatcgc cgcgccgacg atcgtcgatt
     5221 ggcgggccgt ggacgccgag cgcgtgcagc cgggagtcgt cagcgccgcc acgggccgag
     5281 cgggcttcac ttacgtcgag cgggccattg acgcggccct ggccggacag gttgccgccg
     5341 tctcgaccgc gccgctgaac aaggaggccc tgaatgcggc aggcgtccgc tatcccggcc
     5401 acacggaaat cttcgcggac cggaccaagg ccccgcgctc ctgcatgatg cagtactccg
     5461 aggaaatcac ctgcacgttc gtgacggtcc acgtcggcta ccgcgacgtg ccggggctgc
     5521 tcacgaagga gcggatcctg gacgtgatcg agctgacggc cgatgcgctg gcgcgcctgc
     5581 gcagcaagcg gcccaagatc gtcgtctgcg gactcaatcc gcacgccggc gagcacggcc
     5641 tgttcggcga ccgcgaggag gagcggatca tcgtcccggc gatcgaggcg gcccgcgcgc
     5701 aaggcatcga gatcgagggc ccgctgccgc ccgacaccgc gttcctgccc gccaaacgga
     5761 aagcgaccga cgccttcgtc tgcatgtatc acgatcaggg acatatcccg gtcaaggccc
     5821 tcgcgttcga ctcggccgtg aacaccacgc tcggcctgcc gatcgttcgc accagcgtcg
     5881 atcacggcac cgcctgcgac atcgcctggc aggggaaggc caaccccagc agcatcttct
     5941 cggccgtgca gctcgccctg cggctgatcg cggcctgacc ccatgtcgat tgtcatcctg
     6001 gccgacgact tgagtggcgc ggcggaactg gccggcatcg ccttcgcccg cggcctggcg
     6061 accgaagtgc agcggacgtt cgagccggcc agccccgcgg aggtcatcgc cgtcgatacc
     6121 gattcgcgcc atttgcctgc tgccgacgca gccgcccgcg tggctgcggt cgcgcggcaa
     6181 atcgccgcgt cgcagccggc ctggattttc aaaaaggtcg attccgtcct ccgcggcaac
     6241 gtgcgcgtgg agatcgaggc gatcctgaag gccacaggcc agcagcaggc ggtgctggtt
     6301 cccgcgaatc cgtcgcgcgg ccgcacgatc gctcgcggcc aatatctggt ggatggcctg
     6361 ccgctggacc agacgcagtt cgcccaagat cccgagtatc cgcggcggtc ggcccagatc
     6421 gcccatctcc tcggcggcga gtcggcggcg attgccgtgc cagacgttgc ttccctcgac
     6481 gacgttggcc gctgcgcaca ggagcagggc gaccagtctc tggcggctgg cgcagtcgac
     6541 ttcttcgccg cgctgctcga tcggcgcgtc agccgtcccg ctggaagcgc tggttccgcc
     6601 gagttcgtcc tgccgcggcc ggcgctgctc gtctgcggca gccggctgtc ctggcgtcgc
     6661 cgcagcgagg agtgtctcgc cgcgggcgtg agcgtccaga cgctcgccgc cgacttgccg
     6721 agcgccggtt cgcagctggc cgccaccggc cgaatgctgc tcggaatcgg tgacattcag
     6781 tcccctctcg attcgcgcga gctgctttcg cacctggctc agctgacggc ccaggtgatg
     6841 aaccgcacac cgctggccgc actgctcgcc gaaggcggcg cgacggccgc ggccatcgcc
     6901 agcgtcctcg gctggcagcg cttcgccgtg acggcccaag cgccttccgg cgtaggaatt
     6961 ctccagccgc taacttctgc gccggcgccg ctcttgctca tcaagccggg cagctacgcc
     7021 tggcccgaga aaatctggcg gcagtttgcg gcccgctaac ggatacaacc ggccgcgtcc
     7081 gcgcgactgg caggaagcga acgcctcgta caggccgcgc atgctggcgg gtttgccaaa
     7141 gcatgcagct agcgacaatc cagggtcgat gttctgttca acgtcctacc acgctgattc
     7201 tctcgcccgg cggacccatg gcctccacga agcgcaccga aggtacgagc cgcacggccc
     7261 gcaaatccgg ctcgctccag accccgctgg agacctacct ccgcgaaatc aacgaaacgg
     7321 ccctgctcac tgccaaggac gagcaggacc tggctcacct gatcgccttg ggcgacgtgc
     7381 gggcacgcga ccgcatggtg cgggccaacc tgcggctggt ggtcaacatc gcccgcggct
     7441 atacgggcaa gggactcagc ctgcaggacc tgatcgagga ggggaacctc ggtctcttgc
     7501 gagcagtcga ggggttcgac cccgcggtgg gcacgcggtt tagcacgtac gccagctact
     7561 ggatcaagca gtcgatcaag cgggccttga tcaactctgc caagacgatc cgcatcccgg
     7621 cgtacatggt cgagctgctc agcaagtggc ggcgggcgtc ggcacggcta accgaggaac
     7681 tgggccgcac gccgacgccc gaggaagtcg gccgcatcct cggcctgcag aagaaaaagc
     7741 tccccatcat caaaaaggcg atccgcatct acaacagcac gccgcagacc gaccaggccg
     7801 aaaccggctg gtccctcgg
//
LOCUS       DASRJB010000300         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4034096, whole genome shotgun sequence.
ACCESSION   DASRJB010000300 DASRJB010000000
VERSION     DASRJB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034096"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..455)
                     /locus_tag="VFB80_08085"
     CDS             complement(99..455)
                     /locus_tag="VFB80_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33763.1"
                     /translation="MEELAELRDALDRLHAALDDLAIRGLRAAGPQDLAKLAALRDEF
                     HGAGAGHIAGRLDATLEAVRADDRGAAAALLRAMTAARLFDRMLTLEVAASLLSADDA
                     AEEGDAASGDADDGEE"
     gene            complement(485..1564)
                     /locus_tag="VFB80_08090"
     CDS             complement(485..1564)
                     /locus_tag="VFB80_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN33764.1"
                     /translation="MAKSATAKPAATTAADYFVRGDVEQNRQAVKRWNRLTIAQRSGV
                     KFAGADLAGCDLSGINLNGVLGADGASLAAANLSGARLAGSFERASFAGATLTGARLS
                     GCFFEDADFSCAQIAGADLSRSYLECACLTEADLTGANLAECRLHGADFTGAKLTDAV
                     FSGGAFSKRTTWPAGFVPPGDMAWLGPGPDPRLSGKGKRAVAHDINGLMARLHAIIDP
                     ARMKRTLDMLKKEKHQLFFEIEPTMVRGIVRSQKDPDLVYSCVLMEDGIYSCATPDVA
                     KCMGLSDEPCKHILVLVIGLARAGLLDPTTADRWMLAAKTMNPRWNKTVQNYVSDSLL
                     KYKGVMAGEVDWRPAETIPEDFYAM"
     gene            complement(1569..>2466)
                     /locus_tag="VFB80_08095"
     CDS             complement(1569..>2466)
                     /locus_tag="VFB80_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN33765.1"
                     /translation="LRRRIELQGADLSGAKLDKVNMCGYDFKGASFSNSSLIEAELWN
                     SEFPKGVFSGANLERCSFENSVLNGANFQKANLTGANLANSKLLGADFTGAKLKDASF
                     AAAQYDQTTIFPKGFAPPSDMTWKGDGPPPNAKKVKAAKAGSLDFDSFLAQLNTKVEV
                     ERMKKAASMLKAERFQLFAEVKEDSLVGIVKSQTDKDLVYSCRLVNDGAFSCCTQNLR
                     PCGGLRGALCKHLLVLIVGLAKAGQLDAATVDHWIDLSRSQKPAIDEDVMSATFLRYK
                     GAEAGEIDWRPTETIPEDFYAM"
BASE COUNT          398 a          835 c          775 g          458 t
ORIGIN      
        1 cgtggtcagt ccagctagca gcagctcctc aaccgcgccg ctcagttgcg cgagcacggc
       61 ggcgagcttc ggtcgttcgg tggccggcgg aagcatgcct attcctctcc atcatccgcg
      121 tcgccgctgg cggcatcgcc ttcttctgcg gcatcgtccg cggacagcag actcgcggcc
      181 acctcgagcg tcaacatcct gtcgaagagc cgtgcggccg tcatggcgcg gagcagcgcc
      241 gccgccgcgc cgcgatcgtc cgcgcgcacg gcttccagcg tcgcatccag ccgtccggcg
      301 atgtggccag cgccggcgcc atgaaactcg tcgcgcagcg cggccagctt cgccaggtct
      361 tgcgggccgg ccgcgcgcag gccgcgaatc gccagatcgt cgagagcggc gtgcagccga
      421 tcgagggcgt cgcggagctc ggcaagttct tccatgggtc actttggcgc gcgagccctc
      481 agcctcacat cgcgtagaaa tcctcaggaa tcgtctcggc gggccgccaa tcgacctcgc
      541 cggccatcac gcctttgtac ttgagcagcg agtcgctgac gtagttctgc acggtcttgt
      601 tccagcgcgg gttcatcgtc ttggcggcca gcatccaccg atcggcggtg gtcgggtcga
      661 gcagccccgc acgagccaat ccgatgacca gcacgaggat gtgcttgcaa ggctcgtccg
      721 acaaccccat gcacttcgcg acgtccggcg tggcgcacga gtagatgccg tcctccatca
      781 gcacgcagga atacaccagg tccgggtcct tctggctgcg gacgatgccg cggaccatcg
      841 tcggctcgat ctcgaagaag agctgatgct tctccttctt gagcatgtcg agcgtccgct
      901 tcatccgcgc cgggtcgatg atcgcgtgca ggcgggccat caggccgttg atgtcgtggg
      961 caaccgcccg tttgcctttg cccgacagcc gcgggtcggg gcccggcccc agccaggcca
     1021 tgtcgccggg cggcacaaag ccggcgggcc acgtcgtgcg tttgctgaac gcaccgccgg
     1081 aaaacacggc atccgtcagc ttcgcgccgg tgaaatccgc gccgtgcagc cggcactcgg
     1141 ccaggttcgc gccggtgagg tccgcctccg tgaggcaggc gcactcgaga tagctgcgcg
     1201 agaggtctgc gcccgcaatc tgagcgcagg agaaatcggc atcctcgaaa aagcagccgc
     1261 tgagcctcgc gccggtgagc gtcgcgccgg cgaacgaggc cctttcgaag gagcccgcca
     1321 accgagcgcc gctcaggttc gccgcggcca gcgacgcgcc gtctgcaccc agcacgccgt
     1381 tgagattaat gcccgaaagg tcgcagcctg ccagatctgc gccggcgaac ttcacgccgg
     1441 accgctgggc gatggtaagc cggttccacc gttttacggc ctgccggttt tgctcaacat
     1501 cgccgcggac gaagtaatcg gcggcggtgg tggccgctgg tttggctgtc gcgcttttgg
     1561 ccatgctgct acatcgcgta aaaatcttcg ggaatcgtct ccgtcggccg ccagtcgatt
     1621 tcgccggctt ccgcgccttt gtagcgcagg aaggtggcgc tcatgacgtc ttcgtcgatc
     1681 gccggcttct ggctgcggct gagatcgatc cagtgatcga ccgtcgccgc gtcgagctgg
     1741 ccggccttgg ccagcccgac gatcagcacc agcaggtgct tgcacaaggc tccccgcagc
     1801 ccgccgcacg gccgcaggtt ctgggtgcag cagctgaacg cgccgtcgtt gaccagccgg
     1861 caggaataga caaggtcctt gtcggtctgg cttttgacga tgccgacgag cgaatcttcc
     1921 ttcacctcgg cgaacagctg aaaccgctcg gccttgagca tcgacgccgc tttcttcatc
     1981 cgctcgactt cgaccttggt attgagctgc gcgaggaagg aatcgaagtc gagcgagccc
     2041 gccttggccg ccttcacctt cttcgcattc ggcggcggcc cgtcgccttt ccaggtcatg
     2101 tcgctcggcg gcgcgaagcc cttgggaaaa atcgtcgtct gatcgtattg cgccgcggcg
     2161 aagctggcgt ccttcagttt cgcgcccgtg aagtcggcgc cgagcagttt cgaattggcc
     2221 aggttcgcgc cggtcagatt ggccttttgg aagttggcgc cgttgaggac ggaattctcg
     2281 aaactgcagc gttccaaatt cgcccccgaa aagactccct tgggaaactc actgttccac
     2341 agctcggctt cgatcagcga cgagttggag aaactcgcgc ccttgaagtc atatccacac
     2401 atatttactt tgtcgagctt cgcgccgctc aaatcggccc cctgcaattc gatccgccgg
     2461 cgcagg
//
LOCUS       DASRJB010000301         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_86043, whole genome shotgun sequence.
ACCESSION   DASRJB010000301 DASRJB010000000
VERSION     DASRJB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86043"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB80_08100"
     CDS             complement(<1..574)
                     /locus_tag="VFB80_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN33766.1"
                     /translation="MPRLSIVIPCQGGAAEFDATLVSVLQNRPANCEVLVVHTERYDD
                     PYGLAAEVDFQRASCHSLAGLINAGLAQARGEIVHVLGCGLEATEGWTAAATAHFDDA
                     DVAAVSPLIVGPDGQAVVAAGVQFTRGGRRKLVTDRRVLDAGAGRLRASVLGPLLTAA
                     FFRRDVLAALDGLDESIGDELADVEAALSIR"
     gene            complement(751..1395)
                     /locus_tag="VFB80_08105"
     CDS             complement(751..1395)
                     /locus_tag="VFB80_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZN33767.1"
                     /translation="MDRLRKLLQPVELEQHAVVFEAGQAMPHVLFVDEGMISVVSTME
                     NGARIEVGTIGNEGVAGLGAILGVLSTPYRYTVQVAGRGRRLSAEALFAELRGDRQLA
                     RVLYRYSAAFVTQVMQGVACNGLHSVEQRCCRWLLTTQDRLGSQVINITHDFLAQMLG
                     VRRASVTEVLRPLQTEGLIRARRGQVTILDPKRLALSSCECYRVIRNEYERLLG"
     gene            1690..2058
                     /locus_tag="VFB80_08110"
     CDS             1690..2058
                     /locus_tag="VFB80_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN33768.1"
                     /translation="MRILIVDDLVDAADGLTRLLRRLGHDVRTVYDGRAALTLAEQFH
                     PEVVVLDLALPRLDGYQVALGLRRMKGLQSACLIALSGYGQESDIRRGKEAGFDYHLV
                     KPVAIEQILELLSLHFVQPA"
     gene            complement(2070..>3844)
                     /locus_tag="VFB80_08115"
     CDS             complement(2070..>3844)
                     /locus_tag="VFB80_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN33769.1"
                     /translation="GIVMNDVPKPATALPDWHPAWSKELAELYYSGTINTFVLYGNVH
                     DLVRSRKDGQTAFVSLSDFLATQVFGAWDVTLSYDLGRGLRAVAGDDGPRLSAMMQYV
                     TGVIGGPQGWSRDPDKALDLVEPLLQRNLLEDNVAQRKRLALLFEHAQFLLPAGDLNS
                     QARSQAARLVRFLSWAANPYIKRLNIAFCLITEKLSELSERLTNSPHVATIEIPLPTS
                     DERREFIASAAGENSKGKLGSMSIEGLAQNAAGLSLVNLNVLLSQGGSSRPLDLGQFR
                     KLKKSMIERQCQGLVEFVEPPHTLDLLVGQPAAKARLEQDAKWISEGRLDTAPMGYLF
                     CGAVGTGKTFFAECYAGSIGIPCLKLRNFRSKYVGETEGNLEQVLTVLRSLGPVVVIV
                     DEADASLGNRQSDGDSGTSGRVFSMIAQQMGDTRYRGRIIWMLLTCRPDLLPIDLKRQ
                     GRCEVHIPLFAPHDSSEIATMVAAMARKNKVKLAEGAIPEIPANRDLSGADLESILLG
                     ARRKALAAGRETVERGDLESALEEFIPSAQGLEKELQELAAVLECTERNFLPPNWRDR
                     IAQPGARPKLQERLVALRQLVEGD"
BASE COUNT          652 a         1317 c         1235 g          640 t
ORIGIN      
        1 ctcgaatcga aagcgcggcc tcgacatcgg ccagctcgtc gccgatcgat tcatcgaggc
       61 cgtccagcgc cgccagcacg tcgcggcgaa aaaaggcagc agtcagaagc ggaccgagca
      121 cgctggcccg cagccggccc gcgccagcat cgagcacgcg gcgatccgtc accagtttgc
      181 ggcggccgcc gcgcgtgaat tgcacgcctg ccgctacgac ggcctgtccg tccggtccga
      241 cgatcagcgg cgaaaccgcc gcgacgtcgg cgtcgtcgaa gtgcgccgtg gcggcagcgg
      301 tccaaccctc cgtcgcctcc agcccgcagc ccagaacgtg aacgatctcg ccgcgcgctt
      361 gggccagccc ggcgttgatc aggcctgcca ggctgtggca gctcgcgcgc tggaaatcga
      421 cttccgccgc caggccgtac ggatcgtcgt agcgctcggt gtgcaccacc agcacttcgc
      481 agttcgcggg ccggttctgc aacaccgaca cgagcgtggc gtcgaactcc gccgcgccgc
      541 cctggcaggg aatcacgatc gaaagccgag gcacggatcc ttccttctcg atctcggcag
      601 gtgcgacttg gcggtgctag cagccggccg gtgccagccg ctgcgaaaag ccgcctctct
      661 tatcttcggc cgctgccagc gagagcgatt atccaatctg cctgctgcct cggctccccg
      721 cgccaaatga aatccgtctg gcgcgggccc tcatcccagc agccgctcat attcgttgcg
      781 gatcacgcga tagcattcgc acgacgacag cgccaggcgc ttgggatcga ggatcgtgac
      841 ctggccgcgg cgggcgcgga tcagaccctc ggtttgcagc ggccgcagca cctcggtgac
      901 gctggcccgc cgtacgccca gcatctgggc caggaaatcg tgcgtgatat tgatcacctg
      961 cgatcccagc cggtcctgcg tggtgagcag ccagcggcag cagcgctgct cgacggagtg
     1021 caagccgtta caggccacgc cctgcatgac ctgcgtcacg aaggcggcgg agtaacggta
     1081 gagcacccgc gccagctggc ggtcgccccg caattcggcg aagagcgcct cggcgctgag
     1141 ccgccggccg cgtccggcga cttgcacggt gtagcggtag ggggtgctga gcacgcccag
     1201 gatcgcgccc agccccgcga cgccttcgtt gccgatcgtg ccgacttcga tccgcgcgcc
     1261 gttttccatc gtgctcacaa cggagatcat gccttcgtcc acgaagagga cgtgcggcat
     1321 ggcctgcccc gcctcgaaaa ccaccgcatg ctgctccagc tcgacgggct gcaacagctt
     1381 ccgcagccgg tccaactcgg cggggtatag ggaccggagg agctggtttt ccggggctcg
     1441 agattggggg gctgaagtgg acataagttt aaaaatggcc ggtgttcggc acagttgcga
     1501 acaaagtgaa cgatccaagg ccagtcggta cggccccgga cagattgtcc gtgaccgcgc
     1561 cggttatgct ctggcacaaa gcagcacgtc atgtctggca attcccccaa cccgccgcgc
     1621 tcggcgcgta tgcgcaaacc ccggcggtcc agccatctac cgtccgcgcg caagcaagcg
     1681 ctcccccgct tgcgcattct catcgtcgat gatctcgtcg acgcggccga tggtctgacg
     1741 cgtttgctgc gccgactcgg tcacgacgtg cggacggtgt atgacggaag ggcagccttg
     1801 acgctcgccg agcagttcca cccggaggtg gtcgtgctcg acttggcgct gcccaggctc
     1861 gacggctatc aagtcgccct cggcctgcga aggatgaaag ggttgcaaag cgcgtgcctg
     1921 atcgcgctat ccggttacgg gcaggaatcc gatatccgcc gaggcaaaga agcaggcttc
     1981 gactatcacc tggtcaaacc ggtagcaatc gaacagatcc tggagctgct gtcgctgcac
     2041 ttcgttcagc cagcatgacg caggccgact cagtcaccct ccacgagttg gcgcagggcc
     2101 accagccgtt cctggagttt cgggcgggcg ccaggctggg cgatacggtc gcgccagttg
     2161 gggggcagga agttccgctc ggtgcactcc agcacggcgg ccaactcctg caattccttc
     2221 tccagcccct gcgccgatgg aatgaactct tctagcgcgg attccaggtc gccgcgctcg
     2281 accgtttcgc ggccggccgc gagcgccttg cggcgcgcac ccagcaggat gctctccagg
     2341 tccgcgcccg agaggtcgcg gttggccggg atctccggga tcgctccctc ggccagcttg
     2401 accttgttct tcctggccat cgccgcgacc atcgtcgcga tctcggaaga atcgtgcggc
     2461 gcaaagagcg gaatgtgcac ttcgcaccgc ccctgccgct tgaggtcgat cggcaagagg
     2521 tccgggcggc aggtcagcag catccagatg atccgcccgc gatagcgcgt gtcgcccatt
     2581 tgctgggcga tcatcgaaaa gacgcggccg gacgtgcccg agtcaccgtc ggactgccgg
     2641 ttgcccagcg aggcgtcggc ctcatcgacg atcaccacga ccgggcccag cgaacgcagc
     2701 acggtcagca cctgctccag gttgccttcc gtctcgccga cgtatttgga ccggaagttc
     2761 cgcagcttca ggcacggaat gccgatcgag ccggcgtagc attcggcgaa gaacgtcttg
     2821 ccggtgccga ccgcgccgca aaacaggtag cccatcggcg ccgtatcgag ccggccttcg
     2881 ctgatccact tcgcgtcctg ctccagccgg gccttggccg ccggctgccc gaccagcaga
     2941 tccagcgtgt gaggcggctc gacgaactcg accagcccct ggcactgccg ctcgatcatg
     3001 ctcttcttca gcttgcggaa ctggcccagg tccagcggcc ggctgctgcc gccctgcgac
     3061 aggagcacgt tgagattcac cagcgaaagg ccggctgcat tctgcgcgag cccttcgatc
     3121 gacatcgagc cgagcttgcc tttggagttc tcgcccgcgg ccgacgcgat gaattcccgg
     3181 cgctcgtcgc tcgtgggcag cggaatctcg atggtcgcca cgtgcgggct gttggtcagc
     3241 cgctcgctca actccgagag cttctcggtg atcaggcaga aggcgatgtt gagccgcttg
     3301 atgtagggat tggccgccca gctcaggaac cgcacgaggc gggccgcttg cgagcgggcc
     3361 tgcgaattca ggtcgccggc gggcagcagg aactgggcgt gctcgaagag cagcgccaag
     3421 cgcttgcgct gggcgacgtt gtcttccagc aggttgcgct gcaggagcgg ctcgaccagg
     3481 tcgagggcct tgtcgggatc gcgcgaccag ccttgcgggc cgccgatgac gccggtgacg
     3541 tactgcatca tcgccgagag ccgcgggccg tcgtcgccgg cgaccgcccg caatccacgg
     3601 ccgaggtcgt agctcagcgt cacatcccac gcgccgaaga cctgcgtggc cagaaaatcg
     3661 ctcaggctga cgaacgccgt ctgaccatcc ttccgcgagc ggaccaggtc gtgcacgttg
     3721 ccgtagagca cgaacgtgtt gatcgtgccc gagtaataca gctccgccag ctccttgctc
     3781 cacgccggat gccagtcggg caaagccgtg gccggtttgg ggacatcatt catgacaatt
     3841 ccaa
//
LOCUS       DASRJB010000302        14498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1497666, whole genome shotgun sequence.
ACCESSION   DASRJB010000302 DASRJB010000000
VERSION     DASRJB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497666"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1417
                     /locus_tag="VFB80_08120"
     CDS             20..1417
                     /locus_tag="VFB80_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HZN33770.1"
                     /translation="MCKVLLGVTGCVLSLALATAALAQVAPKSKTAPQPATPQPAVTF
                     AQTVQRMSGEKAGIAAKHQALLAERYELADRPSEAVKMSRGKPVQAGPRTKLPQGQSW
                     EQLAALPAAQIREQKLWPAGFLPLPHPNHPEGGMLFPKVHIDEIKKQEGRDLTRFDLD
                     FDLPQHFLPEFPPPIFLTTHPELGDVTKGQLVTIANFFELFKDLLNPKQLDGLRLLVT
                     AFPQQQFNATDDRRSLLAHRGVTCFDCHVNGHANGATHLVGDIRPQEFRHRIETPSLR
                     GVNVQRLFGSQRALKSVEDFTEFEQRAAYFDGDPITATKKGISPLDRGTQVHHMAEFQ
                     ALLDFPPAPKLNVLGKLDEAQASESELRGQALFFGKAKCSECHPAPYYTDNTMHNLKT
                     ERFYSPQTHLGHAAVGDGPIKTFPLRGIKDSPPYLHDGRLLTLEDTIEFFNLVLGTKL
                     TAAEKKDLLAFLQCL"
     gene            complement(1424..1567)
                     /locus_tag="VFB80_08125"
     CDS             complement(1424..1567)
                     /locus_tag="VFB80_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33771.1"
                     /translation="MTDSHQKPRIPAMWYIAGILLGILIAFLVPCCIWPTLVDVWRMT
                     FGG"
     gene            1684..3858
                     /locus_tag="VFB80_08130"
     CDS             1684..3858
                     /locus_tag="VFB80_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN33772.1"
                     /translation="MCRWGYLAAIAFCAVATRPAIAADERPLNFENDIIPILSRYGCN
                     ASGCHGKAEGQNGFKLSVFGFDPPADHRALTVEGRGRRVFPAAPEKSLLLLKASGGMP
                     HGGGVRIAPDRPEYATLVRWIASGMPLGSPTDPHLEKIELSHHEQQLAMGQSLPLAAI
                     ATWSDGRREDVTHLARFQSNSEGLASVDEAGVVTAGQSPGVAAVMASYLGSVDTLHVL
                     IPRPQPKEPLPAVTQHNFIDGHVYRRLSQLNIAPAEVCSDAEFLRRASLDLIGTLPTA
                     EEARKFLAGTEPNKRAVLVERLMERPEFADYWALKWADLLRVERQSLGHKGAYDFYRW
                     LRLSLAENRPLDDIARQIVTAEGPLADVPPANFYSAVKEPGKAASALSQVFLGVRIEC
                     AQCHHHPFDRWSQTDYFGMTAYFAQLQQKNTPLGMTLIALGDPQMAHPRTGEKIAAHA
                     LGQPLPAKNPEGDRRRELAAWMTAPENPYFARNLANRVWAHLLGRGLVEPVDDFRETN
                     PPTNPQLLDALAEHLVRSKFDLRALVRAICASRTYQHAAAPNETNARDEQNYSRALFK
                     RMDAEVLLDAVCQATGVPDKFDGAPAGTRAIELWDSQLEHYFLRLFGRPVRQTACECE
                     RVVEPSVAQVLHLLNSERIDAKLKHDAGRVSELVRREPDNGKLADELYLTVFARYPSD
                     AERMTAVDYLAAAPAGDLSQRRARAEDLLWTMLNTIEFVFNH"
     gene            3870..5213
                     /locus_tag="VFB80_08135"
     CDS             3870..5213
                     /locus_tag="VFB80_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33773.1"
                     /translation="MTTRFCDGVIRRDFIRLGLAGLFGSSLSLPQLLRAESAGKAAKD
                     RGVSVIYVFLKGGLSTIDTLDMKPDSPAEFRGDFDPAPTCVSGIQICSLLPKLAQQLD
                     KVALLRGFTHKNSDHGPADHYMLTGYFPQAGFNPNLNPNNVRPSLGSIISRKLGPQGA
                     ARQAAVVPPYVCLPQTHNSAGPAYLGATCAPLSVEADPNAPDFAVPDMAPPLSLPADR
                     LAARRELLKKVDQLQQSAEVEANSRARAVNVFRQRAFDLILSPAAKQAFNIHAEPEKL
                     RDAYGRSTLGQSCLMARRLVEAGVRCVTIEHSNWDTHDGNFNTLRNQLLPQLDPAIAT
                     LLADLSSRGMLEKTLVLVTGEFGRTPRINKNAGRDHWGPSFTVLLAGGGVKGGRVIGS
                     SDERAERPSDQPHGPEDLFATIAQLVGIDPNEEFFTPEGRPIKVVNNGKFIRGLV"
     gene            5213..7384
                     /locus_tag="VFB80_08140"
     CDS             5213..7384
                     /locus_tag="VFB80_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN33774.1"
                     /translation="MVRRLLAAVAACLLAAGVGWAEPPHASYIFPAGGQRGTKVSVRV
                     GGHFLHERANFEMLGAGATAPAEIRRTETIWFEGPLIKIPASQAAEDYPQDFAAEISL
                     AADAKLGSRWWRCWNAQGVTPPLPFIVGEFPEVVEEEIDGAPLPVAVSLPVTINGRVF
                     PREDVDVWAFEANAGQAIACSAMARDLGSPLVPKLAVYDPAGKLLGENTGGAWAEASL
                     RFVAPQAGRYSIHIADVASGGLQNYVYRLTVSAGSNTRPEAAAGDLVEAEPNEAPATA
                     KAANVPSVLGGRIQSAGDLDCWLLELKKDQPVTFESQLASPLAIVVTVKDASGKQLAQ
                     LDSTTSNAADPALSFTPPADGTYLVEIGERFARRGGPEFAYRLAVAPQAADFRLYAAD
                     SLAIDVGTEKALEVLVERSGGFKSPLTLHVDGLPQGVTCADVQVPPNSNKAALKFQCA
                     KDTPVVSSLLKIVGRADVGGQTVERAALLGRPKDDFGRGEDVPTRLAVTLSTPFKFSG
                     QFSQIYIPRGGVLKKRYTIDRGGFSGPLAARLADRQGRHLQGVTGGVVQIPAEATEFE
                     YPLHLPPWMDLGRTSRTNLMLTGELKDAAGTAHKVCFTTKEQNEQLIALVTASALRLS
                     LDRSAIAVQPNTELKIPVAIKRDGAVQSPITLELVAPRHMRDISAAAVTAPAGASSAT
                     LTIQLGQSPGPLNMPLVIRARGQRQGETITAEEPVELFRLP"
     gene            complement(7394..7891)
                     /locus_tag="VFB80_08145"
     CDS             complement(7394..7891)
                     /locus_tag="VFB80_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN33775.1"
                     /translation="MDRPSRDVGQPGQSLHRIQEVRRLQGMSLRTAARQLGTDIRSIR
                     AQEQASTDLKLSDLYRWQQALEVPVGELLVEDAEPLSRPVRERAAMVKVMKTARTLLE
                     TAPAGGVRRMAENLVEQLLDLMPELKEVSPWHTVGQRRGLDELGRIAEQPISDAPYCC
                     ADACE"
     gene            complement(8189..9016)
                     /locus_tag="VFB80_08150"
     CDS             complement(8189..9016)
                     /locus_tag="VFB80_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN33776.1"
                     /translation="MRSIAVMNQKGGVGKTTTAVNLSAALAAAGQRVCLIDLDPQAHA
                     SLHLGVTLGDDQPSIYHVLTGDKAFQDVRRQVAENLWLVPAHLDLAAAEVELAGEVGR
                     EVILRDKLANDEPFDFLLIDCPPSLGVLTINALTMVREVFLPLQPHFLALHGLSKLLR
                     TIEIVSQRLNRQLKLTGVVLCMYDANTRLANEVSDDVVAYFAKDRTPDCIWAEARTFA
                     TRIRRNVRLAEAPSFGQSILQYAPASHGAEDYQNLAREVLSSAGCSARPQPVLRNAA"
     gene            9314..10099
                     /gene="scpB"
                     /locus_tag="VFB80_08155"
     CDS             9314..10099
                     /gene="scpB"
                     /locus_tag="VFB80_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZN33777.1"
                     /translation="MIPAQQLGSTSPRYAGHGLHVFATAAPRAMPPSASETRVVANSA
                     GDHDPALARLEAVLFLAREPVSSRKLSQYANLADGTQARTLVRRLNERYDAAGRAFRA
                     EQVAGGYQLLSRRKFAPWLRRLAHVPGESRLSAPALETLAVIAYRQPVPRADIEAVRG
                     VNCGEILRQLMERDLVRISGRGEELGRPFLYATTKRFLQLFGLVTLEELPRAAELRAS
                     PLPAAPPIFPRPPEALPDAGTAVDETETENTTESEIDAELTEG"
     gene            10142..10573
                     /locus_tag="VFB80_08160"
     CDS             10142..10573
                     /locus_tag="VFB80_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33778.1"
                     /translation="MTVSTPAFLPIDERRRAAGLPGLAARGARWLGCAAPRLEDDEEE
                     LDDEEMDDDDDEDEDWEDEEDDDLDDDEEDDLDDDEDEDLDDDEDLDDEWEEVGDDDD
                     ELDEEDDDEDEDDDLDDDDEDDDDWDDDEDEEEKEGEGGWE"
     gene            complement(10599..11000)
                     /locus_tag="VFB80_08165"
     CDS             complement(10599..11000)
                     /locus_tag="VFB80_08165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN33779.1"
                     /translation="MAIDYQLEPELPAEEFVDVLVRSTLAERRPVHDLETIRGMLRHA
                     DLIVTARAGDLLVGVSRAITDFSYCTYLSDLAVDRQFQRQGIGRELIRRTQEAAGLAT
                     TLILLSAPAARSYYPHIGMQPHDSCWVIPRR"
     gene            complement(11014..12558)
                     /locus_tag="VFB80_08170"
     CDS             complement(11014..12558)
                     /locus_tag="VFB80_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33780.1"
                     /translation="MRFRLLSLVLALPLLPAAAVAQVSPSGLISTEVARQFGLERMWF
                     THLHVDRTRGRIAGLHMHVSDTLAHTVFQITHDGRRYVFSQRDRDAFGNEIGVEGAQK
                     LADEQLKAIETAMKTAGKNDPLPTIETFIVPQITLYSTSERGTVHSLDAETGRTRWTK
                     SVGRPTLPTTAPSACDKYVGVLNGSTLYIMLSADGSLVWNRPVVGAPGAGPALTDQLA
                     FVPMISGQIETFMLADPKHPAAIYKSFGRAMVQPIVSFNSVAWPTDAGNLYVSLAREP
                     GMRYRVQAKDAINSAPAFLPPDKIFATSLDGYLYCVNERRGNVLWRFTTGEPISHSPV
                     ALGSTAYAITDRGNMFAISASEGKELWVQSGIRSYVAGNDKRLYCIDVGGNLTILETA
                     SGSKLGTIGVGQLDLPLMNVETDRIILASTTGLIQCFRESSLPFPVAHFRSEAAAAAT
                     TTKTPPPPGPTPTPMPMGTDLNDPFGAPKPATPAPPAANPPDPFGAPPAPAAPAAPAA
                     PANPFG"
     gene            12722..14488
                     /locus_tag="VFB80_08175"
     CDS             12722..14488
                     /locus_tag="VFB80_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33781.1"
                     /translation="MDSVLPCPGCQSPLAITPTMAGQVIACPACGQKIQLAGAKKSAS
                     AAMPAVAPTKPKPSSRTPAGPSGASIAILATCFVLGALVVLGGIGAWLWASGQSPKPV
                     AANVAPVKTPAPPPPPPVPPAIPVAQLTPPSATPATPVAPPPVSAAAVVAAAQTPMSL
                     PGVTPAPAPPPFGLPGVPPTPPPPPPYGAPGAPPLPPPFGLPGIPPPSANPTPTAAVT
                     RSATKPTVVQSVAPSGVPTACKLPSLVASGGQSLFSFPSDPGDSLSVSLRSVAADVPK
                     AAAIFLEAGGQPQNWIIGYVSDLNAGAGKLPLATISRQGRDLNFAWDPQRPVDIARQV
                     ANCQLEVRAGVSTYVVQLREPQPLGEVLLDMRTDKQTAEFSFNDWPKGESLRLEIKEL
                     AGFPSEAKMRGDLQAIPLSRSGFIEFPGLPGAEIGLRFYRPAATSSNVVLRLEPVFKE
                     NAVTKHDLTIEHLAELETATRKSLANAKAQLPGAERDLSDAQRALRSLQASKPTNFTE
                     VNVWQQRVTKADSIVKRCSREVAQLRKQIPEYETRLAAVPKIRNFLNDLHQKAAIRYV
                     ITAETGEQDVLLVDGQRPKPAS"
BASE COUNT         2542 a         4809 c         4757 g         2390 t
ORIGIN      
        1 ttgatgcgga gaaactccca tgtgcaaggt tcttcttggc gtgaccggtt gcgtcctttc
       61 gctcgccctc gccactgcag ctctggccca ggtcgcgccc aaatcgaaga cggctccgca
      121 gccggccact ccgcagccgg cagtcacctt tgcccagacg gtccagcgga tgtcgggcga
      181 aaaggcgggc atcgcggcca agcaccaggc gctcctcgcc gaacgctatg agctggccga
      241 ccgcccgagc gaagcggtca aaatgtcgcg gggcaagccg gtgcaggccg ggccgcggac
      301 gaagctgccg cagggccagt cctgggagca gctcgccgcc ctgcccgccg cccagattcg
      361 cgagcagaag ctctggccgg cggggttcct gccgctgcct caccccaatc atccggaagg
      421 gggcatgctc ttccccaagg tccacatcga cgaaatcaag aagcaagagg gacgcgacct
      481 gacccgcttc gatctcgatt tcgatctgcc ccagcatttt ctgccggagt ttccgccgcc
      541 gatcttcctc acgacacatc cggagctggg cgacgtgacc aaggggcagc tcgtgacgat
      601 cgccaacttc tttgagctgt tcaaggacct gctgaatccc aagcagctcg acggcctgcg
      661 gctcctggtc acggcgtttc cgcagcaaca gttcaacgcc accgacgatc gccgttcgct
      721 cttggctcac cgcggagtga cttgcttcga ctgccacgtg aacgggcacg ccaacggcgc
      781 gacgcacctc gtcggcgaca tccgccccca ggaattccgc caccgcatcg agacgcccag
      841 cctccgcggc gtgaacgtcc agcgcctgtt cggctcgcag cgggcgctca agtcggtcga
      901 ggacttcacc gagttcgagc agcgggccgc ctatttcgac ggcgatccga tcacggcgac
      961 gaagaagggc atcagcccgc tcgaccgcgg cacgcaggtg caccacatgg ccgagttcca
     1021 ggcgctgctc gacttcccgc cggccccgaa gctaaacgtg ctcggcaagc tcgacgaggc
     1081 gcaagccagc gagtccgagc tccgcggcca ggccctgttc ttcggcaagg ccaagtgcag
     1141 cgaatgccac cccgccccgt actacaccga caacacgatg cacaacctca agaccgagcg
     1201 gttctattcg ccgcagacgc acctcggtca cgccgccgtc ggcgacgggc cgatcaagac
     1261 gttcccgctc cgcggcatca aggactcgcc gccctacctg cacgacggcc ggctgctgac
     1321 gctcgaagac accatcgagt tcttcaacct cgtcctcggc acgaagctga ctgccgcgga
     1381 gaagaaggac ctgttggcgt tcttgcagtg cctgtgaaca gacctaaccg ccgaacgtca
     1441 tccgccacac gtccaccagc gtgggccaga tgcagcaagg cacgagaaac gcaatcagta
     1501 ttcccagcag tattccggcg atataccaca tcgccggaat gcgcggcttc tggtgagagt
     1561 cggtcatcag ggcgattcta gctggcggcc tatttcgctt gctctcgtga gaggggagca
     1621 ttaacatcca ttgatgtatc ggcccgtgga ttcgcgtccc gcctctcact cccgcctttg
     1681 ccgatgtgcc gctggggata tctcgctgca atcgcattct gcgccgtggc cacgcggccg
     1741 gcgatcgcgg ccgacgagcg gccgctgaac ttcgaaaatg acatcatccc catcctcagc
     1801 cgctacggct gcaatgcctc gggctgccac ggcaaggcgg agggacagaa cggattcaaa
     1861 ctctcggttt ttggcttcga tccgccggcg gaccaccggg cgctgacggt ggaaggccgc
     1921 ggccgccggg tctttccggc agccccggaa aagagcctgc tgttgctcaa ggccagcggc
     1981 ggcatgccgc atggcggcgg cgtgcggatt gctccggacc ggcccgagta tgccacgctc
     2041 gtgcgctgga tcgcgtccgg catgccgctg ggttcgccca ctgatccgca ccttgagaag
     2101 atcgagcttt cacaccacga gcagcaattg gcgatgggcc agtcactgcc gctggccgcc
     2161 atagcgacct ggtcggatgg ccgccgcgag gacgtgacgc acctggcccg ctttcaatcg
     2221 aacagtgaag gcctggcgtc ggtcgatgag gccggcgtag ttaccgccgg acagtcgccg
     2281 ggcgtggccg ccgttatggc cagctatttg ggcagcgtcg atacgctgca cgtgctcatc
     2341 ccacggccgc agccgaagga gcctttgccc gcggtcactc agcacaactt catcgacggc
     2401 cacgtttacc gccggctgtc gcagctgaac atcgccccgg ctgaggtgtg cagcgatgcc
     2461 gagttcctcc gccgcgccag cctcgacctg atcggcacgc tcccgaccgc ggaagaggcg
     2521 cggaagttcc tggcaggcac ggagcccaac aagcgagcag ttctcgtcga gcggctcatg
     2581 gagcggccgg agttcgccga ctattgggca ctgaagtggg ccgatttgct gcgcgtcgag
     2641 cggcagtcgc tcggccacaa gggcgcgtac gatttctatc gctggctgcg gctgtcgctg
     2701 gccgagaacc ggccactgga cgatatcgcc cgccagatcg tgaccgccga agggccgctg
     2761 gccgatgtgc cgcccgcaaa cttctattcg gccgtgaagg agcccggcaa ggcggccagc
     2821 gcgctctcgc aggtgttcct tggcgtgcgg atcgagtgtg cccagtgcca ccatcatccg
     2881 ttcgaccgct ggagccagac cgattatttc ggaatgaccg cctatttcgc gcagcttcag
     2941 caaaagaaca cgccgctggg gatgacgctg atcgccctgg gcgacccgca gatggcgcat
     3001 ccgcggaccg gcgagaagat cgcggcccac gcgctggggc aaccgctgcc ggcgaagaat
     3061 cccgaagggg accgccgccg cgagctggcc gcgtggatga ccgctcccga gaatccgtac
     3121 tttgcccgca acctggctaa ccgcgtctgg gcccatttgc tcggccgcgg gctggtggag
     3181 ccagtcgatg attttcgcga gacgaacccg ccaacgaacc cgcaattgct cgacgcgctg
     3241 gcggagcatc tggtgcggag caagttcgac ctgcgggcgc tagtccgtgc gatctgcgcc
     3301 tctcggacct atcagcatgc cgccgcgccg aacgagacca acgcccgcga tgagcaaaac
     3361 tactcgcggg cgctcttcaa gcggatggat gccgaggtgc tcctggacgc cgtctgccag
     3421 gccaccggcg ttcccgacaa gttcgacggc gcaccggccg gcacgcgggc catcgagctc
     3481 tgggacagcc agctcgagca ctatttcctg cggctgtttg gccggccagt gcggcagact
     3541 gcctgcgagt gcgagcgagt cgttgagccg agcgtggccc aggtcctgca cctgctcaat
     3601 tcggagcgga tcgacgccaa gctaaagcac gacgcgggcc gcgtgtctga actggtccgc
     3661 cgcgagccgg acaatggcaa gctggccgat gagctgtacc tgaccgtgtt tgcccgctat
     3721 cccagcgatg cggagcggat gacggccgtc gattaccttg cggccgcgcc ggccggcgac
     3781 ttgagccagc gccgggcgcg ggccgaagat ctgctctgga cgatgctcaa taccatcgaa
     3841 ttcgtgttca accactagga cgtacgaaca tgaccacacg cttttgcgac ggcgtcatcc
     3901 gccgcgactt tatccgcctg ggcctggccg gcctgttcgg ctcgtcgctc tcgctgccgc
     3961 agctcttgcg ggcggaatcg gcgggcaagg ctgccaaaga ccgcggcgtg tcggtgattt
     4021 acgtcttcct caagggaggc ctgagcacga tcgacacgct ggacatgaag ccggattctc
     4081 ccgcagagtt ccgcggcgac ttcgatcccg cgccgacctg cgtcagtggc atacagattt
     4141 gcagcctcct gcccaagctc gcccagcagc tcgacaaggt agccttgctc cgcggcttta
     4201 cgcacaagaa cagcgaccat gggcccgccg accactacat gctgaccggc tatttcccgc
     4261 aggcgggatt caacccgaac ctgaatccga acaacgtgcg gccgagcctg gggtcgatta
     4321 tcagccgcaa gctcgggccg cagggcgccg cccggcaggc cgcggtggtg ccgccctatg
     4381 tctgcctgcc gcagacgcac aacagcgccg ggcccgcgta cctgggcgcg acctgcgcgc
     4441 cgctttcggt cgaggccgat cccaacgccc ctgatttcgc cgtgcccgac atggccccgc
     4501 ccctcagcct gccggccgac cggctagcgg cgcgccgcga gctgctcaag aaggtcgatc
     4561 agctgcagca gtcggcggag gtggaggcga acagccgggc gcgggcggtc aatgtcttcc
     4621 ggcagcgggc cttcgacttg attctgtcgc cggcggcaaa gcaggcgttc aacattcacg
     4681 ccgagccgga gaagctccgc gacgcctatg gccgctcgac gctcggccaa tcgtgcctga
     4741 tggcccgccg cctggtcgag gcgggcgtgc ggtgcgtgac gatcgagcac agcaactggg
     4801 acacgcacga cggcaatttc aatacgctgc ggaatcagct gctgccgcag ctcgacccgg
     4861 cgattgctac gctgctggcg gacctgtcga gccgcgggat gctggagaag acgctcgtgc
     4921 tcgtcacggg cgaattcggc cgcacgccac gaatcaacaa aaacgccggt cgcgaccact
     4981 gggggccgtc gttcacggtg ctgctggccg gcggcggcgt gaaaggcggc cgcgtgatcg
     5041 gctcgagcga cgagcgggcc gagcggccca gcgatcagcc gcacgggccg gaagacctgt
     5101 tcgccacgat cgcccagctc gtcggcatcg atccgaacga ggagttcttc actcccgaag
     5161 gccggccgat caaggtcgtc aacaacggca agttcatccg ggggctggtg taatggtccg
     5221 ccgtttgctc gcagcggttg cagcctgcct gttggcggcc ggcgtcggct gggctgaacc
     5281 gccgcatgcg tcgtacattt tcccggccgg cggtcagcgc gggacgaagg tcagcgtccg
     5341 cgtcggcgga cattttctgc acgagcgtgc gaacttcgag atgctcggcg cgggtgcaac
     5401 tgcgccagcc gaaatccgac gcaccgagac gatctggttc gagggcccgc tcatcaagat
     5461 tcccgccagc caggcggccg aagactatcc gcaggatttc gccgccgaaa tctcgctcgc
     5521 ggcggacgcg aagctcggca gccgctggtg gcgttgctgg aatgcccagg gcgtgacgcc
     5581 accgctgccg ttcatcgtcg gtgaattccc ggaggtcgtc gaggaagaaa tcgacggcgc
     5641 gccgctgccg gtggccgtct cgctgccggt aacgatcaat ggccgcgtct ttccgcgcga
     5701 ggacgtggac gtctgggcgt tcgaagcgaa tgccggacaa gcgatcgcct gctccgccat
     5761 ggcccgcgat ctgggctcac ccttggtccc caagctggct gtgtacgatc cggcaggcaa
     5821 gctgctgggg gagaacacgg gtggtgcgtg ggccgaagcg agcctgcgat tcgtcgcgcc
     5881 gcaggcaggc cgctacagca ttcacatcgc cgacgtcgcg tccggcggcc tgcaaaacta
     5941 cgtctatcgc ctcaccgtct ccgccgggtc aaatacaagg cccgaggccg cagccggcga
     6001 cctggtggag gcggagccga acgaggcgcc tgcgacggcg aaggccgcaa acgtcccctc
     6061 ggttctcggt ggacggattc aatcggcggg cgacctcgac tgctggttgc tcgagctgaa
     6121 aaaagaccag ccggtcacgt ttgaatcaca gctcgcttcg ccgctggcga tcgtcgtaac
     6181 ggtcaaggac gcatccggca agcagctcgc ccagctcgac agcacgacgt cgaacgcagc
     6241 tgatccggcg ctgagcttca cgccgccggc cgacggcaca tacctggtcg aaatcggcga
     6301 gcggttcgcc cgccgcggcg gaccggagtt cgcctatcgc ctggcggtag cgccgcaggc
     6361 cgccgacttc cgcctctatg cggcggacag cctggcgatt gacgtcggaa cggagaaagc
     6421 gctggaagtg ctcgtcgagc ggagcggcgg attcaaatcg ccgctgacat tgcacgtgga
     6481 cggcttgccc cagggcgtca cgtgcgccga cgtgcaggtt ccacccaatt cgaacaaggc
     6541 ggccctgaaa ttccaatgcg cgaaggatac gccggtcgtt tcgtcgctac tgaaaatcgt
     6601 cggccgcgcg gatgttggcg gccagaccgt cgagcgcgcc gcgctgctag gccgcccgaa
     6661 agacgatttc ggccgcggcg aggacgtgcc gacgcggctg gcggtcacgc tcagcacgcc
     6721 gttcaaattc agcgggcaat tcagccagat ctatatcccc cgcggcggcg tgctgaagaa
     6781 acgctacacg atcgaccgcg gcggctttag cggaccgctc gcagcccgcc tcgccgaccg
     6841 gcaaggccgg cacttgcagg gcgtgaccgg cggcgtggtg cagattcccg cggaagcgac
     6901 ggaattcgag tatccgctgc acttgccgcc ctggatggat ctcggccgca ccagccgcac
     6961 aaatttgatg ctcacgggcg agctgaagga tgccgcgggg acggcgcaca aagtttgctt
     7021 tacgaccaag gagcagaacg agcagctcat cgccctggtc accgcctcgg ccctgcgctt
     7081 gtcgctcgac cgcagtgcca ttgccgttca gccgaacacg gagctgaaga ttcccgtggc
     7141 catcaagcgc gacggtgcgg tccagtcgcc gattacgctg gaactggtcg cgccccggca
     7201 catgcgcgac atttccgccg ctgcggtcac cgcgccggcg ggtgcgtcgt cggcgacact
     7261 gacgatccag ctcggccaga gtcccgggcc gctgaatatg ccgctggtga ttcgcgcccg
     7321 cggtcagcgg caaggcgaaa cgatcaccgc cgaggagccg gtggaactct tcaggctgcc
     7381 ctaagctggc aaattactcg caggcatccg cgcagcagta cggtgcgtcg ctgatcggct
     7441 gctcggcaat gcggcccagc tcgtcgaggc cgcgccgctg gccgaccgtg tgccaggggc
     7501 tgacttcctt cagctcgggc atcaggtcga gcagctgctc gacgaggttc tcggccattc
     7561 gccgcactcc gccggccggg gcggtttcca gcagcgtgcg ggccgtcttc attaccttga
     7621 ccatcgccgc ccgctcgcgc accggccgcg acaggggctc ggcgtcttcg accagcaatt
     7681 cgccgacggg aacttccaac gcctgctgcc agcggtagag atcgctcagc ttgaggtcgg
     7741 tcgaggcctg ctcctgggcc cggatgctgc ggatatcggt gcccagctgc cgggccgcgg
     7801 tccgcagcga catgccttgc aggcggcgga cttcttgaat gcggtgcagc gactgccccg
     7861 gttgtcccac gtcgcgggac ggacgatcca aatcagaata aacaatcgac agctctgcac
     7921 tcgccatggc gacccttgct cctcgctcgc ggactggccg cggctctgtt tccggaaatg
     7981 ccaatttcac aaggagtcgg gccgtcctgc tgtccccctt gtcttgtccg cgcaggccca
     8041 tcgtcggccc gcgcctctgc ggacgcactt tggacgcggg gctattacga aaagttcccc
     8101 aatttcgcaa gcgaattgcc agtccgcggc tcaagaattc tcgaattttg ctaaggccag
     8161 agcgggcctg gagttgcgac gatatagcct aggccgcgtt ccgcaagacg ggctgcggcc
     8221 tggccgagca gccggcagac gacaggacct cgcgggccag attctgatag tcctcggcac
     8281 catgcgaggc aggtgcatac tgaagaatcg attgcccgaa gcttggcgct tcggccagcc
     8341 gtacgttgcg gcgaatcctc gtcgcaaagg tgcgggcctc ggcccagatg cagtcgggcg
     8401 tccggtcctt ggcaaagtag gccaccacgt cgtcgctgac ttcgttggcc agccgcgtat
     8461 tcgcatcgta catgcacagc acgacgccgg tcagcttgag ttggcgattc agccgctggc
     8521 tgacgatctc gatcgtgcgg aggagcttgg agaggccgtg cagagccaga aaatggggct
     8581 gcagcggcag gaacacctcg cggaccatcg tcagggcgtt gatcgtcagc acgcccagcg
     8641 agggcgggca atcgatcagc agaaagtcga acggctcgtc atttgcgagc ttgtcgcgga
     8701 ggatcacttc gcggcctact tcgccggcca gctccacctc ggcggccgcc aggtccagat
     8761 gcgcgggcac aagccacaaa ttctccgcca cttgccggcg gacgtcctga aatgccttgt
     8821 cgccggtcag cacgtgataa atcgacggct gatcgtcacc gagcgtgaca cccaggtgca
     8881 gcgaggcgtg ggcctgcgga tcgaggtcga tcaggcagac ccgctgtccg gccgcggcca
     8941 gggcagcgct caggttcact gcggtcgtcg tcttcccgac gccgcccttc tgattcatca
     9001 cggcaatgga gcgcatccgc atctcccggc acgggctcgc aaagggcggg gattatagga
     9061 agcaacggcc gccgagccca gacgaactgg cgatggcaag ctggtcaatt tgggaaactc
     9121 gtgccaccag cttttgcatc cgcagcggcg tggtattgtc acaacttcaa gcggctgctt
     9181 atgttgcttc gatcgaatcg cggccgctga attgcggtga gtttttcttt actaccctgt
     9241 tcctctagtc gatgaaatcg ggcggctttg ttacgccgct ttcagcatgc gactgagctt
     9301 gatccatcga accgtgattc ctgcccagca gctgggcagc accagcccgc gctatgcggg
     9361 gcacgggctt cacgtgtttg ccactgctgc gccgcgggca atgccgccca gcgcgagcga
     9421 gacgcgagtc gtggcgaatt cggcgggcga ccatgacccc gcgctggcgc ggctggaggc
     9481 ggtgctgttt ctcgcccgcg agcctgtcag cagccgcaag ctcagccagt acgccaactt
     9541 ggcggacggg acgcaagccc gtacactggt ccgccgcttg aatgagcgct acgatgcagc
     9601 cgggagggcg tttcgagcgg aacaggtcgc gggaggctat cagctgctca gccggcggaa
     9661 gttcgccccg tggctgcggc ggctggcgca cgtgccgggc gaaagccgat tgagcgcacc
     9721 cgccctggaa acattggcgg tcatcgccta tcgtcaaccc gttccgcggg ccgatattga
     9781 ggcggtccgc ggcgtcaatt gcggcgagat attgcggcaa ttgatggagc gcgacctggt
     9841 gcgaatcagc gggcggggcg aagagctggg ccgcccgttt ttgtacgcaa cgacgaagcg
     9901 ttttttgcag ttgttcgggc tcgtgacgtt ggaagaattg ccccgcgccg ccgaattgcg
     9961 ggccagcccg ctgcccgccg ccccgccgat ttttccccgg ccgccggaag ccctgcccga
    10021 tgccggtacc gccgttgacg aaaccgaaac agaaaataca actgagtccg aaattgacgc
    10081 cgagttgacg gaaggttgag cctgctgttc cgttgagatt gttcaccacg aggagttcga
    10141 cgtgaccgtt tccacacctg cgtttctgcc gatcgacgaa cgacgccggg ccgctggcct
    10201 gccgggcctc gccgcgcgcg gagcccgctg gctgggatgc gctgcgccac ggctggaaga
    10261 cgacgaggaa gagctcgacg acgaagaaat ggatgacgac gacgacgagg atgaagactg
    10321 ggaagacgaa gaggacgacg atctcgacga cgacgaggag gacgacctgg acgacgacga
    10381 ggatgaagat ctcgacgacg atgaagactt agacgacgaa tgggaagaag tcggcgacga
    10441 cgacgacgag ctagacgaag aggatgatga cgaggacgaa gacgacgacc tcgatgacga
    10501 tgatgaagac gacgacgatt gggacgacga cgaggacgaa gaggagaagg agggcgaagg
    10561 ggggtgggag taaggcgggg agcgagtggt gagtggtgtc acctcctcgg tatcacccag
    10621 catgaatcgt gcggctgcat gccaatgtgc ggatagtagc tgcgggcggc cggggcggag
    10681 agcaaaatca gcgtcgtcgc caggccggcg gcctcctgcg tgcggcggat gagttcgcgg
    10741 ccgatgcctt gccgctgaaa ctggcgatcg accgccagat cggacagata cgtgcagtag
    10801 ctgaagtcgg tgatcgcccg cgagacgccg acgagcaggt cgccggcccg agccgtgacg
    10861 atcaaatccg catgccgcag catgccgcga atcgtctcca gatcgtgcac cggccgccgc
    10921 tcggccagcg tggagcggac gagcacatcg acaaactcct cggcgggcag ctccggctcc
    10981 aactgatagt cgatggccat cggcaggctt agtctagccg aacggattgg caggggctgc
    11041 tggggccgca ggcgcggcgg gcgcgggtgg cgccccgaac ggatcgggcg ggttggcggc
    11101 gggcggcgcg ggagtcgccg gcttgggcgc gccgaacgga tcgttgaggt cagtgcccat
    11161 cggcatcggc gtcggcgtcg gccccggagg cggcggcgtc ttcgtagtgg ttgccgccgc
    11221 ggccgcctcg ctgcggaagt gcgcgacggg gaacggcagg ctcgactccc ggaagcactg
    11281 gatcaggccg gtagtcgaag ccaggatgat ccggtcggtc tcgacattca tcagcggcag
    11341 gtcgagctga cccacgccaa tcgttcccag cttgctgccg ctggccgtct ccagaatcgt
    11401 caggttgccg ccgacgtcga tgcagtacag ccgcttatcg ttcccggcga cgtagctgcg
    11461 aataccgctt tggacccaca gctccttgcc ctcgctggca ctgatcgcga acatgttgcc
    11521 ccggtcggtg atggcgtagg ccgtgctgcc cagggccacc ggcgaatgcg agatcggctc
    11581 gccggtggtg aaccgccaca gcacgttgcc ccgccgctcg ttgacgcaat acaggtagcc
    11641 gtcgagcgac gtggcaaaga tcttgtccgg cggcaggaac gcgggggccg agttgatcgc
    11701 gtccttggcc tgcacccgat accgcatgcc cggctcgcgg gccaggctga cgtacaggtt
    11761 gccggcgtcc gtcggccagg cgacgctatt gaacgagacg atcggctgca ccatggcccg
    11821 gccgaacgac ttataaatcg ccgccgggtg tttggggtcg gccagcatga aagtttcgat
    11881 ctgtccgctg atcattggca cgaacgcgag ctggtccgtc agtgccggac ccgctcccgg
    11941 cgcgccgacc accgggcgat tccagacgag cgagccatct gcggacagca tgatgtagag
    12001 cgtcgagccg ttcagcactc ccacgtattt gtcgcacgcc gagggcgccg tcgtcggaag
    12061 ggttggccgt ccgaccgact tggtccagcg ggtgcggccg gtttcggcgt cgagcgaatg
    12121 gaccgtgccg cgctccgagg tggaatagag ggtgatctgg ggcacgatga acgtctcgat
    12181 cgtcggcagc gggtcattct tgccggcggt cttcatcgcc gtctcgatgg ccttgagctg
    12241 ctcgtcggcc agcttttgcg cgccctcgac gccgatctcg ttcccgaagg cgtcgcggtc
    12301 gcgctgcgaa aacacatacc gccggccgtc gtgcgtgatc tgaaagaccg tgtgggccag
    12361 cgtgtcgctg acgtgcatgt gcagcccggc gattcggccc cgggtgcgat cgacgtgcag
    12421 gtgcgtgaac cacatccgct ccagaccgaa ttgccgggcg acctcggtcg agatcagccc
    12481 cgacgggctg acctgcgcca ccgcggctgc gggcaatagc ggcagggcca ataccagcga
    12541 tagcaggcga aagcgcatag gacgaactcg cgacagcgcg gaggatcagg gataccatcc
    12601 atcctaacca cgccgctagt cgttttcatc gaaaaaatgg ccgccgcagc tgccttggcg
    12661 gtcttgaatt gccaactccc cacgccggtg gtactgtaat tcgcacctgc agagggacat
    12721 catggactcc gtacttcctt gtccgggttg ccagtcgccc ttggcgatta ctcccaccat
    12781 ggccggccag gtgattgcct gcccggcttg cggtcagaag attcagctgg ccggtgcgaa
    12841 gaagtccgcc tcagcggcga tgccggccgt agcgcccacc aagcccaagc cgtcatcccg
    12901 cacgccagct ggcccaagcg gagcttcgat cgcgattctg gccacctgtt ttgtgctggg
    12961 ggcgctggtg gtcctgggcg gcatcggggc ctggctctgg gcatcgggcc agtcgcccaa
    13021 gccagtggcc gcgaatgtag cgccggtcaa aacgccggct ccgccgccac cgccgccggt
    13081 tcctcctgca attcctgtgg cacaactgac tccgccgtcc gctactccgg cgacgccggt
    13141 agccccgccg ccggtttccg ccgctgccgt ggtcgcggcc gcgcaaacgc caatgagcct
    13201 gcctggcgtg acgccagcgc ccgctccacc gccgttcggg ctgccgggcg ttcccccgac
    13261 gcccccaccg cctccaccct atggagcgcc cggcgcgccg ccgctgccgc caccgttcgg
    13321 gctgccgggg attccgcctc cctcggcgaa tccgactccg acggctgcgg taacgcgcag
    13381 cgcgacgaaa ccgacggtcg tccagagtgt agcgccgagt ggagtgccga ccgcctgtaa
    13441 gctgccctcg ctggttgcgt cgggcggtca aagcctgttc tcgttcccca gcgacccggg
    13501 cgattcgctc agcgtctccc tccgctcggt tgccgccgat gtgcccaagg cagccgcgat
    13561 cttcctcgag gccggtgggc agccgcaaaa ctggatcatc ggctacgtca gcgatctgaa
    13621 cgccggggcg ggcaagctgc cgctggcgac catcagccgc cagggccgcg acttgaactt
    13681 tgcctgggat ccgcagcggc ccgtcgatat cgcccggcag gtggccaact gtcagttgga
    13741 ggtgcgggcc ggggtctcga cgtacgtcgt gcagctccgc gagccgcagc cactgggcga
    13801 agtgctgctc gacatgcgga ccgacaagca gacggccgag ttctccttta acgactggcc
    13861 caagggcgag agcctccgcc tcgagatcaa ggaactcgcc ggctttccca gcgaagcgaa
    13921 gatgcgcggc gatctgcaag cgatcccgct gtcgcgctcc ggcttcattg agtttcccgg
    13981 cctgccgggg gcggagatcg gcctgcggtt ctatcggccc gccgccacca gctccaacgt
    14041 ggtcctgcgg ctggagccgg tgttcaagga aaacgcggtc accaagcacg acctgacgat
    14101 cgagcacctg gccgagttgg agaccgccac gaggaagtcg ctggccaatg ccaaggcgca
    14161 gctgccggga gcggagcgcg acctttccga tgcgcagcgc gccttgcgtt cgcttcaggc
    14221 cagcaagccc acgaacttca ccgaggtcaa cgtctggcag caaagggtga ccaaggccga
    14281 cagcatcgtg aagaggtgca gtcgggaagt ggcccagctc cgcaagcaga ttcccgaata
    14341 cgagacccgc ctggccgccg tgcccaagat ccgcaacttt ctgaacgacc tgcaccagaa
    14401 agcggcgatc cgttacgtca tcacggccga gacgggcgag caggacgttt tgctggtgga
    14461 cggccagcgc cccaagccgg cgtcgtagcg ggcttaga
//
LOCUS       DASRJB010000303         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_87350, whole genome shotgun sequence.
ACCESSION   DASRJB010000303 DASRJB010000000
VERSION     DASRJB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87350"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFB80_08180"
     CDS             <1..440
                     /locus_tag="VFB80_08180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN33782.1"
                     /translation="PRRRALVMGVDDLVRKWIGPAQAWAEILPPCALPAREFYRRIHC
                     LMPINFAALENWPRVGLEAMACGVPIVAPREGGWCDIITHGENGFLASSAEEFAELGT
                     LLARDESLRMKIAAQARRKLETELANPDDIWAGWQRLFASLGG"
     gene            497..2314
                     /gene="treZ"
                     /locus_tag="VFB80_08185"
     CDS             497..2314
                     /gene="treZ"
                     /locus_tag="VFB80_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZN33783.1"
                     /translation="MQRDQPPVCGAQPTDDGRCLWRVWAPSVRQVELVLAGPPRSIAM
                     TAAPRGYFEAELPNIAEGQRYAFRLDGSEPRPDPASTWQPDGVHAPSAVFNPRLHAWR
                     DANWPGVAADRLVIYELHVGTFTPEGTFAAIVPRLPQLVELGVTAIELLPVNQFPGHR
                     GWGYDGVHWNAVHNTYGGPRGLQQLVAACHAAGLAVILDVVHNHLGPEGNYLPLFGPY
                     QNTRCRTPWGEGLNYDDAGSDAVRGLVLAGVRQWVRDFHVDGLRLDAVHSIHDYSPRH
                     LLADIKLAADEESQRLGWPVHVIAESDLNDVRLLQPLDHGGYGLDAQWSDDFHHCVHC
                     LLTGERDGYYADYPEPAAQLAKALNETFAYDGCYSAVRQRRHGAPAGDIGGERFIVNI
                     QTHDQVGNRARGERLHQLVGPAQQRLAAGLLLLAPHTPLLFMGEEYGETRPFLYFCNF
                     GDRALQESIRLGRQQEFAEFHWQGAIPDPNDPATFEQSRLCWSWPALSPQGGLRLLYC
                     ELLAARKRWPALGDFRHRRAQLFAGQGGLSLLRGDSARPAEQLLAWFNLTGSPLPLPE
                     ESHPPHLLFRSEEQRYGGASFAGEGLAPWEFIVWGTHRE"
     gene            2307..5420
                     /gene="treY"
                     /locus_tag="VFB80_08190"
     CDS             2307..5420
                     /gene="treY"
                     /locus_tag="VFB80_08190"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZN33784.1"
                     /translation="MSNTLTLEEQPASTMPGSSAPLAATQDGQAAEIESIVAGVGQRI
                     AAQRLPIATYRMQFNRHFTFRQAQELVPYLAALGISDLYASPLLQAREGSLSGYDVVD
                     YGAINSELGTRDELESLSRSLRGAQMGLILDVVPNHMGTGGGRNRWWQDVLENGPSSP
                     YADYFDIDWMPLKPDLAHKILLPVLGDQYGKVLEDGQLSVRLDGGVLWLHCYDSRYPL
                     AVGSYAAVLGALADPLAQQLGAENSELLEFLSILTAIRNLPPQTAATRPQVLEKHREQ
                     DVIKRRLKELLGRSPAIAAAIESGLSSLNGTRGDARSFDRLDALIREQSYRLAYWRVA
                     ADEINYRRFFDINELAAVKMEQREVFENSHALVFELLDRDIVQGLRIDHPDGLFDPRG
                     YLSQLQEKRFLQLARHQWESRQLATAAADAWPAMENRLHELFRLAKENLASPLARPLY
                     VIVEKILGAGEPLPEDWPVHGTVGYKYLCALSGLFVAADAEQPLSAMYTRFSGESLDY
                     RELAYRCKRLIVRMNMSSELAVLGHRLDRLSERNRWTQDFTLSSLTQALQEVIAVFSV
                     YRSYVEEGRVGDGDRRYIESAVARAKRRNPAMSASIFDFVRDLLLLRYRENADEEERT
                     AQRALVGKFQQLTGPIMAKAIEDTAFYRFNRLVSLNEVGGDPVRFGTSAAEFHKLNAE
                     RVQLFAHSLSASSTHDTKRSEDVRARINVLSEIPREWRQHVLRWARWNKRFRTDVEGQ
                     PAPSANDEYLLYQTLVGIWPDEIPAGAARQQLVERLQQYMLKVAREAKSHTSWISPHE
                     PYEQALLQFVAAIFQAEKKRPFVANLHEFARLVADHGRWNSLSQLVLKIASPGAPDFY
                     QGTELWSLTLVDPDNRQSVDFGLRQRLLADLQARDGAGSRAELLRELLEHRQDGRVKL
                     FTTWQALLTRRKWPELFTAGEYLPLEVRGTHAERLIALARRRSGELAIAAVPRLTARV
                     CGFGGPPPLAGAWGDTVVVLPPEIRGRSGLVDCFTQRQYSLATESLRAAELLEQFPAA
                     LLTTGGS"
     gene            5426..>6071
                     /locus_tag="VFB80_08195"
     CDS             5426..>6071
                     /locus_tag="VFB80_08195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZN33785.1"
                     /translation="MDPTRASGVLLHVTSLPSPGGCGDLGREAYDFVGGLARAGQGVW
                     QVLPLGPTGYGDSPYQCFSAFAGNPLLVSLELLEAEGWLAAADLGRAPAFPEAAVDYA
                     AVSAFKRPLLARAAGRFREGAAAADRQAYDAFRDRQAGWLDDFALFMALKDAHRGVAW
                     TDWEPEVAARHPEALARWRQRLETEVESRRVEQFFFFRQWESLRAHAHAQGVRLF"
BASE COUNT          914 a         2120 c         2080 g          957 t
ORIGIN      
        1 acccgcggcg gcgggcgctg gtgatgggcg tggacgacct cgtccgcaaa tggatcggtc
       61 ccgcgcaggc ctgggcagag atccttccgc cttgtgcact gcctgcccgc gaattctatc
      121 gccgcatcca ttgcctgatg ccaatcaact ttgcggcgct cgagaactgg ccccgcgtcg
      181 ggctggaggc gatggcctgt ggcgtgccga tcgtcgcgcc gcgcgagggc ggttggtgcg
      241 acatcattac gcacggcgaa aacggctttc tggcgtccag cgcggaggag tttgccgagc
      301 tgggaacgct gctggcccgc gacgagtcgc tgcggatgaa gatcgccgct caggcccgcc
      361 gcaagctcga aaccgagctg gccaatcccg acgacatctg ggccggctgg cagcgactgt
      421 tcgccagcct gggcgggtga cctcccgcgg cggcgcggcg tagaatggag tttgccgcga
      481 tcatccggga gtgatcatgc agcgcgacca gccgccggtg tgcggcgctc agcccaccga
      541 cgacggccgc tgcctgtggc gggtctgggc gcccagcgtc cggcaggtgg aactggtcct
      601 cgctggtccg ccccgcagca tcgcgatgac cgccgccccg cggggctact tcgaagccga
      661 gctgccgaat atcgccgaag ggcagcgcta tgcctttcgc ctcgatggca gcgagccgcg
      721 gcccgatccg gcctcaacct ggcagcccga cggcgttcat gcgccctcgg ccgtcttcaa
      781 cccgcggctg cacgcatggc gcgacgcgaa ctggcccggg gtcgccgccg accggctcgt
      841 gatctacgag ctgcacgtcg gcacgttcac gcccgaaggc actttcgcgg cgatcgtccc
      901 gcggctgccg cagctggtcg agctgggcgt gacggcgatt gagctgttgc cggtgaacca
      961 gtttccgggc caccgcggct ggggctacga cggcgtgcac tggaacgcgg tccacaacac
     1021 ctacggcggg ccgcgcgggc tgcagcagct cgtcgccgcc tgccacgccg cggggctggc
     1081 cgtaatcctc gacgtggtcc acaaccacct cggccccgag gggaactatc tgccgctgtt
     1141 cgggccgtac caaaacacgc gctgtcgcac gccctggggc gaggggctca actacgacga
     1201 tgccggctcc gacgccgtcc gcgggctcgt gctggctggc gtgcggcagt gggtccgcga
     1261 cttccacgtg gacggcctgc ggctggacgc cgtgcactcc atccacgact acagcccgcg
     1321 gcacctgctg gccgacatca agctcgccgc cgacgaggaa tcgcagcggc tgggctggcc
     1381 ggtccacgtc atcgccgaga gcgacctcaa cgacgtccgc ctgttgcagc cgctcgacca
     1441 cggcggctac ggcctcgacg cccagtggag cgacgacttt caccactgcg tccactgcct
     1501 gctgaccggc gaacgcgacg gctattacgc cgactatccc gagcccgcgg cgcagctcgc
     1561 caaggccctc aacgagacct tcgcttacga cggctgctac agcgccgtcc gccagcggcg
     1621 tcacggagcg ccggccggcg acatcggcgg cgagcggttc atcgtcaaca ttcagacgca
     1681 cgaccaggtc ggcaatcgcg cccgcggcga gcgcctgcac cagctggtcg gcccggccca
     1741 gcagcggctg gccgcgggcc tgctcctgct cgcgccgcac acgccgctct tgttcatggg
     1801 ggaggaatac ggcgagacgc ggccgttcct ctatttctgc aacttcggcg atcgggcgct
     1861 gcaggagtcg atccgcctgg gccggcagca ggagttcgcc gagtttcact ggcagggggc
     1921 gattcccgat cccaacgacc ccgcgacgtt cgagcaatcg cgactgtgct ggtcgtggcc
     1981 cgccctctcg ccgcagggtg gcctgcgcct gctgtattgc gaactgctgg cggcccgcaa
     2041 gcgctggccg gcgctgggcg acttccggca ccgccgggcg cagctgtttg ctggtcaagg
     2101 cgggctcagc ctgctgcggg gcgattcagc tcggccggcc gagcaattgc tggcctggtt
     2161 taatctcacg ggctcgccgc tgccgctgcc ggaggagagt catccaccgc acctgctgtt
     2221 ccgctcggag gaacagcgct acggcggcgc gagttttgca ggtgaggggc tcgcgccctg
     2281 ggagttcatc gtctggggta cgcatcgtga gtaacaccct gacactcgaa gagcagccgg
     2341 caagcacgat gcctggctct tccgccccgc tagccgccac tcaagacggc caggctgccg
     2401 agatcgagtc gatcgtggcc ggcgtcggcc agcggattgc cgcccagcga ctgcccattg
     2461 ccacctatcg catgcagttc aaccggcact tcacgtttcg ccaggcccag gagctcgttc
     2521 cctatctcgc cgcgctcggc atcagcgacc tgtatgcttc gccgctcttg caggcccgcg
     2581 aagggagcct cagcggctac gacgtcgtcg attacggggc gatcaacagc gagctcggca
     2641 cgcgcgacga attggaatcg ctgtcgcgct cgctgcgggg agcgcagatg gggctgatcc
     2701 tcgacgtcgt gcccaatcac atgggcacgg gcggcggccg caaccgctgg tggcaggacg
     2761 tgctggaaaa cggccccagc tcgccttacg ccgattactt cgacatcgac tggatgccgc
     2821 tcaagccgga cctggcccac aagatcctgc tgccggtgct gggcgaccag tatggcaagg
     2881 tgctcgaaga cggccagctc agcgtgcggc tggacggcgg cgtgctgtgg ctgcactgtt
     2941 acgacagccg ctatccgctg gcggtcggct cgtatgccgc cgtcctgggc gcgctggccg
     3001 atccgctggc gcagcagctg ggcgcggaga actcggagct gctggagttc ctgagcatcc
     3061 tcacggcaat ccggaacctg ccgccgcaga cggcggccac tcggccacaa gtcctggaaa
     3121 agcaccgcga gcaggacgtt atcaagcggc gcttgaagga actcctcggc cgctcgcccg
     3181 ccatcgccgc ggcgatcgag agcggcctgt cgtcgctgaa cggcacgcgc ggcgacgccc
     3241 gcagcttcga ccggctcgat gccctgatcc gcgaacagtc gtatcgcctg gcctattggc
     3301 gggtcgcggc cgacgaaatc aactatcgcc gcttcttcga catcaacgaa ctggccgcgg
     3361 tcaagatgga gcaacgcgag gtcttcgaga actcgcacgc cctggtgttc gagctgctcg
     3421 accgcgacat cgtgcagggg ctgcggatcg accatcccga cggcctgttc gacccgcgcg
     3481 gctacctgag ccagctccag gaaaagcggt tcctccagct ggcccggcat cagtgggaat
     3541 cgcggcagct ggcgaccgct gcggccgacg cctggccggc gatggaaaac cgcctccacg
     3601 agctcttccg cctggccaag gaaaacctgg cctcgccgct ggcccggccg ctatatgtta
     3661 tcgtcgagaa aatcctcggc gcgggcgagc cgctgccgga ggactggccc gtgcacggca
     3721 ccgtgggcta caagtacctc tgcgccctga gcggcctgtt cgtggcggcc gacgccgagc
     3781 agccgttgtc ggcgatgtac acgcgcttca gcggcgaatc gctcgactac cgcgagctgg
     3841 cctatcgctg caagcggctg atcgtgcgga tgaacatgtc cagcgagctg gcggtgctcg
     3901 gccaccggct cgaccggctg agcgagcgga accggtggac gcaggacttc acgctcagca
     3961 gtctgacgca ggccttgcag gaggtcatcg ccgtcttcag cgtctatcgc agctacgtcg
     4021 aagagggccg tgtcggcgac ggcgaccggc ggtatatcga gtcggcggtg gcccgggcca
     4081 agcgccgcaa cccggcgatg agcgcgtcga tcttcgattt cgtccgcgac ctcttgctct
     4141 tgcggtatcg agagaacgcc gacgaagaag agcggacggc gcagcgggcc ctggtcggca
     4201 agttccagca gctgaccggg ccgatcatgg ccaaggccat cgaggatacg gccttctacc
     4261 gcttcaaccg gctggtgtcg ctgaacgaag tcggtggcga tccggtgcgg ttcggcactt
     4321 cggcggccga atttcacaag ctcaacgccg agcgcgtgca gctgtttgcc cacagcctgt
     4381 cggccagctc cacgcacgac actaagcgga gcgaggacgt gcgggcccgg atcaacgtcc
     4441 tcagcgaaat cccgcgcgag tggcggcagc acgtcctccg ttgggcgcgg tggaacaagc
     4501 gcttccgcac cgacgtcgaa ggccagcccg ccccgtcggc caacgacgaa tacctgctct
     4561 accagacgct ggttggcatc tggcccgacg aaatccccgc cggcgcggcc cgccagcagc
     4621 tcgtcgagcg attgcagcag tacatgctca aggtcgctcg cgaagcgaag tcgcacacca
     4681 gctggatcag cccgcacgaa ccgtacgagc aggccctgct gcagttcgtg gccgcgatct
     4741 tccaggccga gaagaagcgc ccgttcgtgg cgaatttgca cgagttcgcc cggctcgtgg
     4801 ccgaccacgg ccgctggaat tcgctgagcc agctcgtgct caagatcgcc tcgccgggcg
     4861 cgcccgactt ctatcaaggc accgagctct ggtcgctcac actcgtcgat cccgataacc
     4921 gccagagcgt cgatttcggg ctgcggcagc ggctgctggc cgacttgcaa gctcgtgacg
     4981 gcgctggctc gcgggcggag ctgctgcgcg agctgctcga acaccgccag gacggccggg
     5041 tcaagctgtt cacgacgtgg caggcccttc tcacacggcg gaaatggccc gagctgttta
     5101 ccgcgggcga gtacctgccg ctggaagtcc gcggcacgca cgccgagagg ctcatcgccc
     5161 tggcccgccg ccgcagtggc gagctggcga ttgccgccgt gccgcggctg accgcgcggg
     5221 tctgcggctt tggcggcccg ccgccgctgg ctggcgcctg gggcgatacg gtcgtcgtgc
     5281 tgccgccgga gatccgcgga cgctctggtc tggttgactg ctttacccag cggcaatact
     5341 cattggcgac ggagtcgctg cgcgcggccg aattgctgga gcagttcccg gccgcgctct
     5401 tgaccaccgg agggagctag tcgccgtgga tccgacgcgg gccagcggag tcttgctgca
     5461 cgtcacctcg ctgcccagcc ccggcggctg cggcgatttg gggcgcgagg cctacgactt
     5521 tgtcggcggc ctggcccgcg ccggccaggg cgtctggcag gtgctgcccc tggggccgac
     5581 cggctacggc gactcgccct accagtgttt ctccgcgttc gcgggcaacc ccctgctggt
     5641 cagcctggag ctgctggaag cagaggggtg gctggccgcg gcggacctgg gccgcgcgcc
     5701 cgcgttcccg gaggccgcgg tggactacgc tgcggtcagc gccttcaagc ggccgctgct
     5761 ggcgcgggcc gccggccgct tccgcgaggg cgccgccgcc gcggaccgcc aggcgtacga
     5821 cgccttccgc gaccgccagg cggggtggct cgacgacttc gcgctgttca tggccctgaa
     5881 ggacgcccac cgcggcgtgg cctggaccga ttgggagccg gaggtggccg cgcggcatcc
     5941 ggaggcgctg gcccgctggc gccagcgcct ggagacggag gtggagtcgc ggcgggtcga
     6001 gcagttcttc ttcttccgcc aatgggagtc gctgcgcgcg cacgcccacg cccagggggt
     6061 gcgcttgttc g
//
LOCUS       DASRJB010000304         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_649934, whole genome shotgun sequence.
ACCESSION   DASRJB010000304 DASRJB010000000
VERSION     DASRJB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649934"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1149)
                     /locus_tag="VFB80_08200"
     CDS             complement(334..1149)
                     /locus_tag="VFB80_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN33786.1"
                     /translation="MRLGISSYTFVWAVGVPGYPQPAQPLSAAGLIARAAELGVRVVQ
                     IADNLPLHQLPVAELQALAAQAHERGIDLEVGACGIRPDHLLAYLQLAGDLRSPLLRV
                     VIDAEGQRPGVNDVVQSLRQVLPQFERAGVTLAIENHDRFRAATLASLMQRCESPQLG
                     ICLDTANSLGAGEDVDTVLRAVGRWVVNLHVKDFTARRLPHHKGFVVEGCPTGKGLLD
                     IRRLLTDLKALGRDSNVILELWPPPDATIEESVAKEAAWAVQSLAYLRKLVPE"
     gene            complement(1140..1277)
                     /locus_tag="VFB80_08205"
     CDS             complement(1140..1277)
                     /locus_tag="VFB80_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33787.1"
                     /translation="MFLACADSSFNAAGLIFADAAALIDPSRTLLPGGEHTVSKEPAC
                     V"
     gene            1507..2292
                     /locus_tag="VFB80_08210"
     CDS             1507..2292
                     /locus_tag="VFB80_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33788.1"
                     /translation="MRTVTLAAFLALLGWTATSIVADDAEPAWRTIPLVENGKLAEGW
                     AHIGWGRMTPADGVLRTDCDERGMGLLVYKREKLGDCQLRIVYRPQDAKSNAGVFIRM
                     DDGILDWIGKPSTAVKREASGKLSPAMLDKLKAASEAEEGVWYAVHHGFEVQIMDSAD
                     AGHRTGAIYSLATAAELPAPASEGAWRTMIVTLDGTQVRVSLDGKEVTRFDSAAKDLP
                     PRKNWTEPKREAARPARGYIGLQNHDPGDVVYFKEVAVRPLPR"
     gene            2313..>2593
                     /locus_tag="VFB80_08215"
     CDS             2313..>2593
                     /locus_tag="VFB80_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN33789.1"
                     /translation="MRYLATIMTLALPFAVPMTLRAGMIGLDTSHVPAFAKIFNSEAA
                     AGDVAGIKIVAGYPGGTDIPASRDRVARFTEQIRGMGIEIVDTIPKLLE"
BASE COUNT          463 a          866 c          836 g          428 t
ORIGIN      
        1 cgcgatcgtt ggcaggctgt gggtcacaac gcacctcgag gcggctttag ctgacccctg
       61 aaaaggtggt cgcgcgctgc cttgtttcgc tttttcagtc tgtatcttct ttctggccat
      121 ggacttacgt caaacactcg aaaacgcgat aactactact tattacggtt agagaacgcc
      181 ttgctgccgg ccccgccagc ggccgcgcga atgccgattt gacaggtcac agcgcaacag
      241 gaacaaaaat gcaaatatca taccattgtt cacgttccac caattttcgt cgggccttgt
      301 cagcggttgt aaaggggcgt gatccccgga gcttcactct ggcacgagct tccgcagata
      361 agcaaggctc tgcaccgccc acgcggcttc cttggcgacc gactcctcga tggtggcgtc
      421 gggcggcggc cacagctcca ggatcacgtt cgaatcgcgg ccgagggcct tcaggtcggt
      481 caacaggcgg cggatgtcga gcagcccttt gccggtcggg cagccttcga cgacgaagcc
      541 cttgtgatgc ggcagccgcc gggcggtgaa gtccttgacg tgcagattga cgacccaccg
      601 gcccacagcc cgcagcacgg tatccacgtc ctcgccggcc ccgagcgagt tggccgtatc
      661 gaggcagatg cccagctgcg ggctttcgca gcgctgcatg aggctggcca gcgtcgcggc
      721 gcggaagcgg tcgtgattct cgatggccag cgtcacgccg gcccgctcga actgcggcaa
      781 gacttgccgc aggctctgca ccacgtcgtt cacgcccggc cgctgaccct ccgcatcgat
      841 gacgacgcgg agcagcggcg agcgcaggtc gccggccaat tgcaagtagg ccagcaagtg
      901 atcgggccgg atgccgcagg caccaacttc caggtcaatg ccgcgttcat gggcctgcgc
      961 ggcaagcgcc tgaagctcgg ctaccggcaa ctgatggagc ggcaggttgt cggcgatctg
     1021 cacgacgcgc acgcccagct ccgcggcccg cgcgatcagc ccggccgcgc tcagcggctg
     1081 cgccggctgt ggatatccgg gcacgccgac ggcccagacg aacgtgtatg agctgatgcc
     1141 taaacgcatg ccggctcctt ggaaaccgtg tgctccccgc ccggcagcag cgtccggctg
     1201 ggatcgatca gggcggccgc atcggcgaag atcagccccg cggcgttgaa gctagagtcg
     1261 gcgcaagcca gaaacaagac ggcacatcct gcaatacccg ctagtgggac ggggcggggc
     1321 gttctaccgc ggcggccgct cgcccgtggc tgggaaccgc gcggatcggg ttcctctgga
     1381 aacgcggggc ctacctggcc cacgcgacgc gctcgctggc cgattccatc cggcaatgca
     1441 tcccgctcgc cggtcgctag tttccgcgct atactcgtcc agcactacct ttccgaggtg
     1501 gaaaccatgc gcactgttac tctcgccgca tttctcgccc tgctcggttg gacggccacc
     1561 tcgattgtcg cggacgacgc ggagcccgcc tggcggacga ttccgctcgt cgaaaacggc
     1621 aagctggccg agggctgggc ccacatcggc tggggccgga tgacgcccgc cgacggcgtc
     1681 ctgcggaccg actgcgacga gcgcggcatg gggctgctgg tctacaagcg ggaaaagctg
     1741 ggcgactgcc agctccgcat tgtctatcgc ccccaggatg caaagtccaa cgcgggagtc
     1801 ttcatccgca tggacgacgg cattctcgac tggatcggca agccgtccac ggcggtcaag
     1861 cgcgaggcta gcggcaagct atcgccggcg atgctggaca agctgaaggc cgcgtcggaa
     1921 gccgaggaag gcgtctggta cgccgtgcat cacggcttcg aagtgcagat catggacagc
     1981 gccgacgcgg gccatcgcac cggggcgatc tactcgctgg cgactgcggc ggagttgccg
     2041 gcgccggcca gcgagggcgc ctggcggacg atgatcgtca cgctcgacgg cacgcaagtc
     2101 cgcgtcagcc tcgacgggaa ggaagttacg cgattcgact ccgccgccaa ggatttgccg
     2161 ccgcggaaaa actggaccga acccaagcgc gaggccgccc gtccggcgcg cggctacatc
     2221 ggcctgcaaa accacgatcc cggcgacgtg gtctatttca aggaagtcgc cgtccgcccg
     2281 ctgccgcgat aattctgcac tcggagggta acatgcgata cctggccacc atcatgacgc
     2341 tcgcacttcc gtttgccgtt ccgatgaccc tccgggccgg catgatcggg ctcgatactt
     2401 cgcacgtgcc ggcgttcgcc aagattttca acagcgaggc cgccgcgggc gacgtggcgg
     2461 ggatcaagat cgtcgcgggc tatcccggcg gcaccgacat tcccgccagc cgcgaccgcg
     2521 tggcccgctt caccgagcag attcgcggca tgggcatcga gatcgtggat acgatcccca
     2581 agctgctgga aaa
//
LOCUS       DASRJB010000305         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2054758, whole genome shotgun sequence.
ACCESSION   DASRJB010000305 DASRJB010000000
VERSION     DASRJB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054758"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..546)
                     /locus_tag="VFB80_08220"
     CDS             complement(280..546)
                     /locus_tag="VFB80_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33790.1"
                     /translation="MTPKPLPPKLKRFPPAKQRRMDSLLDKQSEGTISAAERARLAQL
                     VAEAEELMVANGKLLAAFAAGDAPAPAPNAVPVTVWVSPSPAGR"
     gene            complement(642..1955)
                     /locus_tag="VFB80_08225"
     CDS             complement(642..1955)
                     /locus_tag="VFB80_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HZN33791.1"
                     /translation="MNGSEIEQSSVATVELEEPPGSAAYSTPSYSYKLPLFSPPEADS
                     SAPAPREARISRSEMPRRRAVNTRTRAFRRAFYPAVPDKDWNDWRWQTRHRVRKLDQI
                     EKMLVLSNDEREALVKGESMLPVGITPYYMSLLDRDDPLQPLRRTVIPVTGEFLRTPG
                     EADDPLGEDGHSPTPGLVHRYPDRVLLLALDFCSTYCRYCTRSRVVGHGEIMPSESRL
                     EKCFDYIRNTPQIRDVLISGGDPLALSEEKLDWILGNLRAIPHLEFIRIGTKMPAVLP
                     QRITPQLIRVFKKYHPLWMSVHFMHPDECTPEANVACSRLADAGIPLGSQTVLLKGVN
                     DSVGVMQDLCHRLLMMRVRPYYIYQCDPISGSAHFRTSVSKGLEIIEGLRGHTTGYAV
                     PTYVIDAPGGGGKIPLQPNYIVGRDGDDLLLRNYEGQVYRYPDPA"
     gene            2280..3629
                     /locus_tag="VFB80_08230"
     CDS             2280..3629
                     /locus_tag="VFB80_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN33792.1"
                     /translation="MSSPDSPSAADLPAAPAGPPPDASRRDLVRLLALFGFLYFVQGV
                     IEPTANLPYQPLRTQLDRWSYSPGEVGRFFAIIGIAWSLKPFFGLLSDFFPLGGRRRR
                     PYLVLSSLLAAAAFLLLAVTWNDRIAGSRIGEWGLWLLGPSPIAWAGWVMIAGGFGIA
                     MSDVVLDALLVEEGQARGITGQLQSVQWGTMSAASLLTGVLGGYIASYGWLRPMFAGC
                     GALGLVSLAAAALFVREPPRPPGPAGSFWAAIAELTDRRRLWPLLAAAAFLFLWNFNP
                     FYSNVLNHYMEHELHLGDVFYGYTASLSAAAQVVASIAYFNYCRRLSPGLLVHGTIVF
                     GILSILVYGLLHDAASAVVASLVFGFANQTATLTQLDLCARICPRNSTGTTFALLMAI
                     SNTGIAAGINLGGEWYEQLTVRLGSPHAAFDALVLIGAAFTAACWLLVPVLKRSGLR"
     gene            3696..>4145
                     /locus_tag="VFB80_08235"
     CDS             3696..>4145
                     /locus_tag="VFB80_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33793.1"
                     /translation="MTQAGSTSTKRRWSPRLQFSLRLLLAAFTAFAIGFPIWYRWPYT
                     EEEVRYPGKRDPSGQWKQDTTKPPMWRIVRTFRRTWGGGKVQEGPESAYLPLDGTLLS
                     VTHYLANEKHGKHLGYQRYVDPSAPGGVVTVLGLTGTYDHGTKEGRWE"
BASE COUNT          660 a         1421 c         1301 g          763 t
ORIGIN      
        1 ccagcccagc agccacggct caaagccgcg cgggttgaat agcggctgca tctgaccgct
       61 cggatcgccg acctcggttc tctcgttctt tgccaggttg cagcccggac aactcaaagc
      121 caaattgctg ggccgcgtca ggccgccacg cgagcgcggc agcacgtgtt cgatgtgaaa
      181 agtcacaccg gtggcgattt cgacgaggcg gcaatattcg cacgcaccgc cggccctggc
      241 cacgacaaac tggcgaagag aactcgcccg gggcatgctt tatcgtcccg cgggcgacgg
      301 ggatacccac acggttacgg gcacggcatt aggggctgga gcgggcgcgt cgcctgccgc
      361 gaaagcggcc aacaactttc cattcgccac catgagctcc tcggcttcgg cgaccaactg
      421 cgccagccgc gcccgttcgg cggccgaaat cgtcccttcg ctctgcttgt ccagcaacga
      481 gtccatccgc cgctgcttgg cgggcggaaa tctcttgagc ttgggtggga gtggcttggg
      541 cgtcataaac gccgccagag gtgagttacc aatacttatc tccaggatac tttacctagc
      601 gactgcgctc taccaagctc cgctctgcgc cttcgcgcct ttcacgccgg gtcggggtac
      661 cggtacacct gcccttcgta gttccgcagc aacaggtcgt cgccgtcgcg gccgacgatg
      721 tagttgggct ggagcggaat cttgccgccg ccgccgggag cgtcgatcac gtaggtcggc
      781 acggcgtagc ccgtcgtgtg gccgcggagg ccttcgatga tctccagccc cttgctcacg
      841 ctggtccgga agtgggcgga gccgctgatc gggtcgcact gatagatgta gtacggccgg
      901 acccgcatca tcagcagccg gtggcagagg tcctgcatca cgccgacgct gtcgttcacc
      961 cccttcagca gcacggtctg tgagccgagc ggaatgcccg catcggccaa ccggctgcag
     1021 gccacgttgg cctcgggcgt gcattcgtcg ggatgcatga aatgcacgct catccacagc
     1081 ggatggtact tcttgaacac gcggatcagc tgcggcgtga tccgctgcgg cagcaccgcc
     1141 ggcatcttgg tgccgatgcg gatgaactcc aggtgcggga tcgcccgcaa gttgccgaga
     1201 atccagtcga gcttctcttc ggacagcgcc agcggatcgc cgccgctgat cagcacgtcg
     1261 cggatctgcg gcgtattgcg gatgtagtca aagcacttct ccagccggga ctcgctgggc
     1321 atgatctcgc cgtgaccgac gacccgcgac cgcgtgcagt agcggcaata ggtcgagcag
     1381 aagtccagcg cgagcaacag cacgcggtcc ggatagcggt ggaccaggcc tggcgtcggg
     1441 ctgtgcccgt cctcgcccag cggatcgtcg gcctcgcccg gcgtccgcaa aaactcgccc
     1501 gtcacgggaa tcaccgtccg ccgcagcggt tgcagcggat cgtcccggtc gagcaggctc
     1561 atgtagtagg gcgtgatgcc caccggcagc atcgactcgc ctttgaccag cgcctcgcgc
     1621 tcgtcgttcg acaggaccag catcttttcg atctggtcga gcttgcggac gcggtgccgc
     1681 gtctgccacc gccagtcgtt ccagtctttg tcgggcaccg ccggataaaa cgcccgccga
     1741 aacgcccgcg tgcgcgtatt caccgcgcgg cggcggggca tctcgcttcg cgaaatgcgt
     1801 gcctcgcgcg gagcgggagc gctactatcc gcctccggcg gcgaaaacag cggcaacttg
     1861 taggaataag aaggtgtcga ataggcggca ctacccggag gctcttccaa ttcgaccgtg
     1921 gcaaccgacg actgctcgat ttccgagccg ttcataaagg ggaaatcccc aggagccaat
     1981 ccacgctacc gacgccgcat cggtctcctt gccggacggc gggcgatcgc ctacacagcg
     2041 accggttgcg gaaagggcgg gtgcagaatt cgcggagagc cgcgaccaga cgccgggcgg
     2101 cgcaaatcga gccggcgggg gcgctcgtcg cccgctcttg gcaccaggct ctatcatgtg
     2161 gccgtccata ccctgttccc cacgcaacgt gcgcgagagt attgacgcgg aattaaacgt
     2221 tcatgctggc atgctgtcaa tggttttcac cgagcagctg cccagatttg ccaaaattca
     2281 tgtcttcgcc cgattcgccc tcggcagccg acttgcccgc cgccccggcg ggtccgccgc
     2341 cggacgcttc ccgccgcgat ctcgtccgcc tgctggccct cttcggcttc ctctatttcg
     2401 tgcagggcgt catcgagccc accgccaacc tgccctatca gccgctgcgg acccagctcg
     2461 accgctggtc ctattcgcct ggcgaagtcg gccgattctt cgccattatc ggcatcgcct
     2521 ggtcgctcaa gccctttttt gggctgcttt cggacttttt tccactgggc ggccgccgcc
     2581 ggaggcccta cctggtcctc tcctcgctgc tggccgccgc cgcctttctg ctgctggccg
     2641 tcacctggaa cgaccgcatc gccggcagcc ggatcggcga gtggggcctg tggctgctgg
     2701 ggcccagccc catcgcctgg gccggctggg tgatgatcgc cggcggtttt ggcatcgcca
     2761 tgagcgacgt ggtcctcgac gccctgctgg tcgaggaggg gcaggcgcgg ggcattaccg
     2821 gccagctcca gtccgttcaa tggggcacga tgtccgcggc ctcgctgctg accggcgtcc
     2881 tgggcggata catcgcctcc tacggctggc tgcggccgat gtttgccggt tgcggcgctt
     2941 taggcctcgt ttcgctggct gccgctgcgc tcttcgtccg cgagccgccg cggccgccag
     3001 ggcccgccgg cagcttctgg gcggcgattg ccgagctgac cgaccgccgc cggctgtggc
     3061 cgctcctggc cgctgccgcg ttcctgtttc tgtggaactt caacccgttc tacagcaacg
     3121 tgctgaacca ctacatggaa cacgagctgc acctgggcga cgtcttctac ggctacacgg
     3181 cctcgctcag cgccgcggcg caggtcgtcg ccagcatcgc ctacttcaac tattgccggc
     3241 ggctgtcgcc cgggctgctc gtgcacggca cgattgtctt cggcatcctc tcgattctcg
     3301 tctacgggtt gctgcacgac gccgccagtg cggtcgtggc cagcctggtg ttcggctttg
     3361 ccaaccagac ggcgacgctg acgcagctcg acctctgcgc ccgcatctgc ccgcggaaca
     3421 gcaccggcac gacgttcgcg ctgctgatgg ccatcagcaa taccggcatc gcagccggca
     3481 ttaacctcgg cggcgagtgg tacgagcagt tgaccgtgcg gctcggcagc ccccacgccg
     3541 ccttcgacgc cctcgtcctc atcggcgcag ccttcaccgc cgcctgctgg ctgctcgtgc
     3601 cggtgctgaa acgctcgggt ctgcgatgat cgccaccggc tcggcgtgtt acgctacccg
     3661 aatggcaggc tgttgattct gccgccagag gacccatgac ccaggccggt tcaacatcga
     3721 cgaagcgccg ctggtccccg cggctgcagt tcagcctccg cctattgctc gccgcgttca
     3781 ccgccttcgc catcggtttt ccgatctggt atcgctggcc gtacaccgag gaggaagtgc
     3841 gatatccggg caagcgcgac ccctccggcc agtggaagca agatacaacg aagccgccta
     3901 tgtggcgaat cgtccgcaca tttcgccgca cttggggcgg gggcaaagtg caggaaggac
     3961 ctgaaagcgc gtacctgccg ctcgacggaa cgctgctgtc ggtgactcac tacctggcga
     4021 acgaaaagca cgggaaacac ctcggatatc agcgctatgt ggatccgtct gctccagggg
     4081 gcgtcgtgac cgtattaggc ctgaccggca cctatgacca cggcacgaag gagggccgtt
     4141 gggaa
//
LOCUS       DASRJB010000306        10089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3189215, whole genome shotgun sequence.
ACCESSION   DASRJB010000306 DASRJB010000000
VERSION     DASRJB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189215"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VFB80_08240"
     CDS             complement(<1..87)
                     /locus_tag="VFB80_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN33794.1"
                     /translation="MKNVLAVILAGGKGTRLEPLTRDRAKPAV"
     gene            333..578
                     /locus_tag="VFB80_08245"
     CDS             333..578
                     /locus_tag="VFB80_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33795.1"
                     /translation="MATNVRIQSASYAKLRQLANSAGVPMPQVLAEAIDELYRKRFLE
                     ECNRAYSRLRADPKAWKEELDDRRVWDNTLRDGLEDH"
     gene            578..919
                     /locus_tag="VFB80_08250"
     CDS             578..919
                     /locus_tag="VFB80_08250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZN33796.1"
                     /translation="MITPSRAEIWNADLDPTRGREQAGRRPVLVVSTDGFNHGPAELV
                     AVLPVTTKDKRIPWHVPVKAGTAGLTRDSYVMCESVRCISRERLVKQLGDVPSAIMAE
                     VELRLRMLLEL"
     gene            complement(921..2879)
                     /gene="asnB"
                     /locus_tag="VFB80_08255"
     CDS             complement(921..2879)
                     /gene="asnB"
                     /locus_tag="VFB80_08255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN33797.1"
                     /translation="MCGITGAIWTEAERAVSPETLQRMTDCLRHRGPDADGTYYSEFR
                     CRPPYESMPGVALGHRRLSIIDLATGGQPMCNEDGSVWIVFNGEAYNYQALRYRLEGS
                     GHKFRTDSDTETIIHLYEDEGPACFQHLNGMFAVALWDANRRRLVLGRDRMGKKPLVY
                     RHEPGRLIFASELKSLLEVPGLPREIDPAAVDEYLAYQYVPHPNTIFRGYSKLPPGHY
                     AVYQDDKLEVRPYWQPDLALERRIGKQQAIGELRELLESAVKLRMRSDVPLGAFLSGG
                     VDSSIIVALMQQQSQTPVKTFTIGFSEEEYDESKFAERVARHLKTEHRVLEVRPDALE
                     VLPKLAYHYDEPFADSSAIPTWYVSQLTREHVTVSLSGDGGDELFAGYPRYRAAALAG
                     RFDRFPPLRSLAASSWWQWLPSSGRQKSLFRKIKRFSEALALPPERRYLNWVGIFPER
                     MRGELYRDEFLSQLTSDPASFLRNAWRRCQGRDAVTCASLADMQTYLVCDIMNKVDIA
                     SMAHGLECRAPLLDHRVVEFAAALPVRLKYRRGRGKWLLREAFGHLVPREVFTRKKMG
                     FSVPLDYWFRNELKPLAADLLLSPLARCHEFLRPEAIQSLWDAHQEKRYDHSSRLWSL
                     VMLEMWLRQWGGTLALPEPAPQPTAVVT"
     gene            complement(2958..3368)
                     /locus_tag="VFB80_08260"
     CDS             complement(2958..3368)
                     /locus_tag="VFB80_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33798.1"
                     /translation="MPRLTQADLQAYLDEALSPAEMAAIEAELRKQPELLKQLAAINA
                     RRDAGVHSVGEIWRRQRLSCPSREQLGSYVLGALDGGMAEYVEFHLQKIGCRLCLANL
                     EDLRRQQQENSETVKTRRTKYFQSTAGHLRRKKS"
     gene            complement(3389..4033)
                     /locus_tag="VFB80_08265"
     CDS             complement(3389..4033)
                     /locus_tag="VFB80_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33799.1"
                     /translation="MTAPRDADSLLVERIRRGEGEAWDDLIARYEGRLLAFVESRLGR
                     RGPSEDIVQESFVGFLNSLPNYDGRRPLESYLFSICAYKLTDHLRREGRRPAVPISSA
                     SDTSGDWQVSDSARRASSIARSSERKQLEEKAIEGALREQIDRWREKAEWTKLMCAEL
                     LFVRGWANKDVAEQLSITEQQVANYKFDFIARLRTLIKRQDLSEEVFPELYADG"
     gene            complement(4097..4978)
                     /locus_tag="VFB80_08270"
     CDS             complement(4097..4978)
                     /locus_tag="VFB80_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33800.1"
                     /translation="MLTEIQILLTLGLAGAALVAAWPLFRLPRGRRILDGLSGTGLIA
                     AGATLFGLVAGGFIEVPELTSRATAQEPQAEADDAPATATAASEPLATETPAAGTEPK
                     IDLPTFVAPASRPAWINQPQNVSGDGIHTVPVASDPHAREADARRALDRALEAATSEY
                     IFEQLGSQLAPRLLHYDANTIKRRFVKHENLYSDKATYLSGDMYESFALVEFDKSFRD
                     ELAGRWQSIRGQSRLAQVGLFAGAALLLLASVFGYFRLDNATRGYYTGRLQFLTAAAI
                     LAVIGAGVFAAQWIHWL"
     gene            complement(5054..7045)
                     /locus_tag="VFB80_08275"
     CDS             complement(5054..7045)
                     /locus_tag="VFB80_08275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33801.1"
                     /translation="MKFFYASGTRPLEGYTLKRGIGRGGFGEVYFATSDAGKEVAVKH
                     IERNLEVELRGVGQCLNLKHPNLIDLYDIRYDEQGDAWVVMEYVAGQSLKDTVDRNPN
                     GLPLDQVNFWFRGIASGVAYLHDHGIVHRDLKPGNIFEDSGFVKIGDYGLSKFISCSR
                     RSGQTESVGTFHYMAPEIGKGVYGKEIDIYALGIVLYEMITGSVPFDGESSQEIIMKH
                     LTAEAELSGVPQPYRAIIQRALQKDPEKRYHSAAEMLAALNPFAPSAGTKPGPGAAPT
                     RVNGSNGAPAEYKLVPSEPIYIGDESEGIEFGELQLDPSKIVVNAEIVGRNGARTPLP
                     ATTEPVARAAQSSWKRFAQWWDHGRMSTPVKILMLVVAAVIAAVQGGWLLVAAVAAGS
                     FYLVFSGFRLVAGSLRRSPVATAAAAAGSAPAQVPRSAGWHPIAWEQSGRQLLREKTA
                     GDRIGELTGSMLGAALISLVLAVAMAAIGGEALDDSPRAIAGPVWLGLMTTLGSWFVL
                     GAGKWFEASKGEAVRRRFAMLALGLAFGAIAFASAQHLMVRMGDDSVRGMGLFDSMYD
                     SSSRLPTLPAFLGYFGALFLTIGWWKQTDPLRSSRLKIGPILIAILAAWLWLFVFPFP
                     QPWGFMLAAAISISAQLSAPWLSPAERTAAMHRQGGTVV"
     gene            complement(7522..7734)
                     /locus_tag="VFB80_08280"
     CDS             complement(7522..7734)
                     /locus_tag="VFB80_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33802.1"
                     /translation="MQKLVTIYLDNSAYGKGKMLVTSYSEVHGLVEEHLQNYLAEGWQ
                     IKSVGGFGGNSDSLSVRGWAIVVLEK"
     gene            complement(7741..9123)
                     /locus_tag="VFB80_08285"
     CDS             complement(7741..9123)
                     /locus_tag="VFB80_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN33803.1"
                     /translation="MFPPWRFKLREAQVALEQGRLEEAARLASDPQLKQFLPVQQLLA
                     QIGEQFARRALERAASGDFTAGWRDLDEARSLAGETGSWQRLQQAVADVAVGDIVQHL
                     TASDYPGATSRIESLEKRGVPGLPLKTLLEVTRRLDSARKLSQKGKFSEADEQLAAAQ
                     ALRPDLKLIEAQRERCRERQERSKTLTEQLHAALAASDWTKVLSLATELLEISPENRV
                     ARDARKRAWGEVGEQIGDSRRLGETKHWTGTPISGPVTVGGADVPVKRSPRFMLWIDA
                     VGGYLVCLGDEIIIGQAVPGGKVDVPIQADISRKHVKLVRHKEGYILEPLGGKVVLSG
                     KAVTEPALLSDGDEFTLGERVKMRFRKPHVLSSSARLELLSPHRTLPYADAVVLMGES
                     CVLGPQWQNHVVCREFGGDVVLYRSEDKIMCRAMESIEIDGKLHDGRGPVRPGSHVLG
                     TDFSLSLEAV"
     gene            complement(9186..9458)
                     /locus_tag="VFB80_08290"
     CDS             complement(9186..9458)
                     /locus_tag="VFB80_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33804.1"
                     /translation="MNDDVIMGLSAMAILLVLPALWIVCHYTYLCWKQSQSTALIRDM
                     IARGFTPEEIIQLCQALGHRTPRHLKCAGDAPPAKPIRQPAYSVSP"
     gene            complement(9487..9765)
                     /locus_tag="VFB80_08295"
     CDS             complement(9487..9765)
                     /locus_tag="VFB80_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33805.1"
                     /translation="MREEEMVVALAGIFGVFGLPFLACILWLVLHYIYAAFKTWQETR
                     LKRDMIARGYTVQEIVEIVSARRGKSKSGTSNVPPAKPIKYPAYAANT"
     gene            complement(9902..>10089)
                     /locus_tag="VFB80_08300"
     CDS             complement(9902..>10089)
                     /locus_tag="VFB80_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33806.1"
                     /translation="MKDISTRIDVAEKLVSADTSSVGAINLDEEQTSGNIADQVTAYF
                     EASKKADATVAVKLEAE"
BASE COUNT         1742 a         3266 c         3254 g         1827 t
ORIGIN      
        1 cacggccggc ttggcgcggt cgcgggtcag cggttccagc cgcgtgccct tgccgccggc
       61 caggatcacc gccaacacgt tcttcatgat gctgctccgt gatgcccgcg gcccaccacc
      121 gctggcgcac cgattctagc cgcccgcgcc aggcggcgca aggcggcgga agatacgaac
      181 ctgaagcgta agcgaacgtc ttcgcagtgc gacgttggac tccatccgtt cctggcctgc
      241 tgtccgcccg atctccgccc ctgtcgcacg atttgtttgc aggctcgtta acaagaacgt
      301 atactttagc gttgacaatt tcggatgcat ccatggctac taacgttcgc atccaatccg
      361 ccagctatgc caagctgcga caactggcga actcagccgg cgtcccgatg ccgcaggtac
      421 tcgccgaggc catcgacgag ttgtatcgca agcgattcct cgaggagtgc aaccgcgctt
      481 attcccgctt gcgtgcggat cccaaggcct ggaaggagga actggacgac cggcgtgtct
      541 gggataacac gctgagagat gggttggagg atcattgatg attactccct cccgcgcgga
      601 aatctggaat gccgacctcg atcccacgcg gggtcgagag caggcgggcc ggcggccagt
      661 actggtcgta tctaccgacg gcttcaacca cggacccgcc gagctggtgg cggtccttcc
      721 cgtcacaacg aaggacaagc gaataccgtg gcatgttccg gtaaaggctg gtaccgccgg
      781 cctgactcgc gacagctacg tcatgtgcga gtcggtgcgc tgcatcagtc gcgaacgtct
      841 cgtcaaacaa ttgggcgacg ttccatcagc catcatggcc gaagtggaac tgcggctgcg
      901 gatgctgctg gagctgtgat tcacgtcacc accgccgtcg gctgcggcgc gggctcgggc
      961 agcgccagcg tgcctcccca ttggcggagc cacatctcga gcatcaccag cgaccagagg
     1021 cggctagagt ggtcgtagcg tttctcctgg tgcgcgtccc acagcgactg gatcgcttcg
     1081 gggcggagga actcgtggca ccgggccagc ggcgagagca gcagatccgc cgccagcggc
     1141 ttcagctcgt tgcggaacca gtagtccagc ggcacgctga agcccatctt cttccgcgtg
     1201 aagacttccc gcggcaccag gtggccgaag gcttcgcgca agagccactt gccgcggccg
     1261 cggcgatact tcagccgcac cggcagggcc gcggcgaatt cgaccacgcg atggtcgagc
     1321 agaggcgcgc ggcattccag gccgtgcgcc atcgacgcga tgtcgacctt attcatgatg
     1381 tcgcagacca gatacgtctg catgtcggcc agcgacgcgc aggtcaccgc gtcgcggccc
     1441 tggcaccgcc gccaggcatt ccgcaggaag ctggccggat cggacgtgag ctggctcagg
     1501 aactcgtcgc ggtaaagttc gccccgcatg cgctccggga agatgccgac ccaattgaga
     1561 tagcgccgct ccggcggcag ggccagcgcc tcgctgaacc gcttgatttt gcggaacagc
     1621 gacttctgcc ttcccgacga cggcagccac tgccaccagc tcgaggccgc cagcgaccgc
     1681 agcggcggga agcggtcgaa ccggccggcc agcgccgcgg cccggtatcg cgggtagccg
     1741 gcgaacaatt catcgccgcc gtcgcccgac agcgaaaccg tcacgtgctc gcgcgtgagc
     1801 tgcgagacat accacgtcgg aatcgccgag ctgtccgcaa acggctcgtc gtagtggtac
     1861 gccagcttcg gcagcacctc caaagcgtcc ggccgcacct ccagcacgcg atgctccgtc
     1921 ttcagatgcc tggcgacgcg ttcggcgaac ttcgattcgt cgtattcctc ttccgaaaag
     1981 ccaatcgtga aggtcttgac cggcgtctgc gactgctgct gcatgagggc tacaatgatc
     2041 gacgaatcta cgccgcccga aagaaacgcc cccagcggca cgtcgctccg catccgcagc
     2101 ttcaccgccg actccagcag ctcccgcagc tcgccaatcg cctgctgctt gccgatccgc
     2161 cgctccaggg cgaggtccgg ctgccaatac ggccgcacct ccagcttgtc gtcctgatag
     2221 acggcatagt gtcccggcgg cagcttcgaa tagccgcgga agatcgtgtt cggatgcggg
     2281 acgtactggt aggccaggta ctcatcgacg gctgccgggt cgatctcccg cggcaggccg
     2341 ggcacttcca gcaggctctt cagctcgctg gcaaaaatca gccggccggg ctcgtggcga
     2401 tagaccagcg gctttttgcc cattcgatcg cggccgagca ccagccggcg gcggttggcg
     2461 tcccacaggg ccacggcgaa catgccgttg aggtgctgga agcacgccgg gccttcgtcc
     2521 tcgtaaaggt ggatgatcgt ctcggtgtcg ctgtcggtgc ggaacttgtg ccccgagcct
     2581 tccagccggt atcgcagggc ctgatagttg taagcctcgc cgttgaagac gatccagacg
     2641 ctgccatcct cgttgcacat cggctgcccg cccgtcgcca ggtcgatgat cgacaagcgg
     2701 cggtggccca gcgcgacgcc cggcatcgat tcgtacggcg ggcgacagcg gaactcgctg
     2761 taatacgtcc cgtccgcatc cggcccgcga tgccgcaggc agtcggtcat ccgctgcagc
     2821 gtttctggac tgacagcccg ctccgcttcc gtccagattg ctccggtaat gccgcacatg
     2881 ccgcgagtcg ggggtcgggg taacgggagt cgggagtcag gggtcagcgg ctccggaatc
     2941 ggagttggcg gctggcccta cgatttcttt cggcgcaagt ggccggcggt ggattggaag
     3001 tatttggtgc ggcgcgtctt caccgtctcg ctgttctctt gctgctggcg gcggaggtcc
     3061 tcgaggttgg ccaggcagag tcggcagccg atcttctgga ggtggaattc gacatactcc
     3121 gccatgccgc cgtcgagcgc gcccagcacg tagctgccga gctgctcgcg cgaggggcag
     3181 ctcagccgct gccgccgcca gatttcgccg acgctgtgca cgcccgcgtc gcggcgagcg
     3241 ttgatcgccg ccagctgctt gagcagctcc ggctgcttgc gcagctcggc ctcgatcgcc
     3301 gccatctcgg ccggcgaaag cgcctcgtcg agataggctt gcaggtcggc ttgggtcagg
     3361 cggggcatgt ggcaatttgg gatttggatt aaccgtcggc atacaactcc ggaaacactt
     3421 cttccgacaa atcctggcgc ttgatgagcg ttctaagccg tgcgatgaag tcgaatttgt
     3481 agttggcgac ctgctgctcg gtgatcgaca gctgttcggc gacgtccttg ttggcccagc
     3541 cgcggacgaa gagcagttcg gcgcacatca gcttagtcca ctctgccttt tcgcgccagc
     3601 ggtcgatctg ctcgcggagc gcgccctcga tggccttctc ttccagctgc ttgcgctcgc
     3661 tgctgcgggc gatgctgctg gcccggcggg cgctgtcgga gacctgccag tcgccggaag
     3721 tgtcggacgc actgctgatc ggcacggcgg gccggcggcc ttcgcgccgc aggtggtcgg
     3781 tgagcttgta ggcgcagatc gagaacaggt agctttccag cggccggcgg ccgtcgtagt
     3841 tcggcaggct gttcaagaac ccgacgaacg actcctgcac gatgtcttcg ctggggccgc
     3901 ggcggcccag ccggctctcg acgaacgcca gcagccggcc ttcatagcgg gcaatcaagt
     3961 cgtcccaggc ctcgccttcg ccgcggcgaa tccgctcgac cagcagggag tcggcatcgc
     4021 gcggagcggt catggaatcg tagggtaagc gtcctcgctt gcccaggcat gcacaagcgg
     4081 gacgcttatg ctacggctac agccaatgaa tccactgggc tgcgaagact ccagcgccga
     4141 tcactgccag tatcgctgcc gccgtaagga actgcaagcg tcccgtgtag tagccgcggg
     4201 tcgcgttgtc gaggcggaag tagccgaaga ccgacgccag cagcaagagc gccgcgccgg
     4261 caaacaggcc gacctgtgcc aggcggctct gaccgcggat gctctgccac cggccggcca
     4321 gctcgtcgcg gaaggacttg tcgaactcca cgagcgcaaa gctctcgtac atgtcaccgg
     4381 aaagatacgt cgccttgtcg gagtacaggt tctcgtgctt cacgaaccgc cgcttgatcg
     4441 tattggcgtc gtagtgcaag aggcgcggcg cgagctggct gccgagctgc tcgaagatgt
     4501 actcgctcgt cgccgcctcc agcgcccgat cgagcgcccg gcgggcgtcg gcctcgcggg
     4561 cgtgcggatc ggaggccacc ggaaccgtgt gaattccgtc gccgctcacg ttctgaggtt
     4621 gattgatcca agccggccgg ctggctggag cgacgaacgt gggcaggtcg atcttcggct
     4681 cggtgccggc ggccggagtc tcagtggcca gaggctcact tgcggcggtg gccgtggcgg
     4741 gcgcatcgtc cgcctcggcc tgtggttcct gggcggtcgc ccgcgaagta agctcgggga
     4801 cttcgatgaa accgcccgcg accaggccga acaacgtcgc cccggcggcg atcagcccgg
     4861 tgccgctcag gccgtcgaga attcgccgcc cccgcggcaa gcgaaagagc ggccacgcgg
     4921 ctacaagcgc cgcgccggcc agcccgagtg tgagcaggat ctgaatttca gtgagcatga
     4981 ttgcctcctc gtttaacccg gagccggaga gggctgcgtc tggcggggcg gagcctgcgc
     5041 ctccggctac gggttaaacg actgtgcctc cctgccggtg catggctgcc gtccgttcgg
     5101 ccgggctgag ccagggggcg gaaagctgcg ccgagatcga aatcgcggcg gcgagcataa
     5161 agccccacgg ctgcgggaac ggaaacacga acaaccacag ccacgcggcc aggatcgcta
     5221 tcaatatcgg tccgatcttc aatcgcgatg agcgaagcgg atcggtctgc ttccaccagc
     5281 cgatggtcag gaacaaagcg ccgaagtatc ccaaaaacgc cggcagcgtc ggcaagcggc
     5341 tgctggagtc gtacatgctg tcgaacaggc ccatgccgcg gaccgagtcg tcgcccatcc
     5401 gcaccatcag gtgctgagcg gacgcaaagg caatcgcgcc gaaggccagg cccagggcca
     5461 gcatcgcgaa ccgccggcgg accgcctcgc ccttcgacgc ttcgaaccat ttgccagcgc
     5521 cgaggacgaa ccaggagccg agcgtggtca tcagccccag ccagaccggc ccggcgatcg
     5581 cgcggggaga gtcgtcgagg gcttcgccgc cgatcgcggc catcgccacc gccaggacta
     5641 atgaaatcag cgccgcgccg agcatcgagc cggtaagttc gccgatccgg tcgccggcgg
     5701 ttttttcgcg caagagctgc cggccggatt gttcccaggc aatcgggtgc cagcccgcgc
     5761 tgcgaggaac ctgagccggc gccgatcccg cggctgccgc tgccgtagcg acaggcgagc
     5821 ggcggagcga gccggcgacc aggcggaagc ccgaaaagac gaggtagaag ctgcctgcgg
     5881 cgaccgcggc cacgagcagc cagccaccct gaaccgcagc gattaccgct gcgacaacca
     5941 gcatcaggat tttcaccggc gtggacattc gcccgtggtc ccaccactgg gcgaagcgct
     6001 tccagctcga ttgagccgcc cgggcgaccg gctcagttgt agccggcagg ggcgtgcgtg
     6061 cgccgttgcg gccgacgatt tccgcgttca cgacgatctt ggacgggtcg agttgcaatt
     6121 cgccgaactc gattccctcg gactcgtcgc cgatatagat cggctccgac gggacgagct
     6181 tgtattcggc cggagcgcca ttcgagccat tgacgcgagt tggcgccgcg ccgggaccag
     6241 gtttggtccc ggctgatggc gcaaagggat tcaaagcggc gagcatttcg gccgcgctgt
     6301 ggtagcgctt ctcggggtcc ttctgcagcg ctcgctggat gatcgcccga tacggctgcg
     6361 gcaccccgga cagctccgcc tcggccgtca ggtgcttcat gatgatttcc tggctgctct
     6421 cgccgtcgaa cggcacgctg ccggtgatca tctcgtagag cacgattccc agggcataaa
     6481 tgtcgatctc tttgccgtaa acccctttgc cgatctcggg ggccatgtag tggaacgtgc
     6541 cgacgctttc ggtctggccg cttcgccggc tgcacgagat gaacttcgag aggccgtagt
     6601 cgccgatctt gacgaagccg gagtcttcga aaatgttgcc gggcttgagg tcgcggtgga
     6661 cgatgccgtg gtcgtgcagg taggcgacgc cagatgcgat cccgcggaac cagaagttga
     6721 cctggtcgag cggcaggccg ttgggattgc ggtcgacggt gtccttcaag ctttggcccg
     6781 cgacgtattc catcacgacc caggcgtcgc cctgttcgtc gtagcggatg tcgtacaggt
     6841 cgatcaggtt gggatgcttg aggttgaggc actgcccgac gccgcgcagc tcgacctcca
     6901 agttccgctc gatgtgcttg acggcgactt ccttgcccgc gtcgctggtg gcgaaataga
     6961 cctcgccgaa gccgccgcgg ccaatgccgc gcttgagggt gtaaccctca agcggacgag
     7021 tgccgctggc atagaaaaac ttcatggcac cgcctcggac aggggcggcc gcctcggcag
     7081 ccgccgggcc gccggcatcg atcgtagcgg ctcgggaaat catgagacta gtttaccccc
     7141 gcagcgaagc ggcaccatca gcacagacgg ctttgccgcg tctagtgggt tttcggcaga
     7201 cgaatcacaa tcttggacca gtcaaaaacg ccctgagcgt ttggttgtaa gtggttgatg
     7261 cgaacgggct tgtaaagcta ttgaagccta acgcgcaggc gcagaaattc ggacaccttt
     7321 gcgcgcgctt caaccattgg atacctacgc tgagcacaca cggattcatc gtattcccga
     7381 tcaggtcact cgatgcatta tgcatcgtgg gagtttttct gcgactgccg tttgttccta
     7441 agtcgttttg caccaatgat ttggggcggt ttccggctta ttcccttatg cattttatgc
     7501 aacttaagca tcgcgtatgc atcacttctc gagcaccaca atcgcccagc cgcgtacgct
     7561 cagcgagtcg gaattgccgc cgaagccgcc gactgatttg atctgccatc cctccgccag
     7621 gtaattctgc aagtgctcct cgacgagccc atgtacctcg ctgtagctcg tgacgagcat
     7681 tttgcctttc ccataggcac tgttgtcgag gtaaatggtc acgagtttct gcatttgttg
     7741 tcagaccgct tccaaactca gtgaaaaatc cgtccccagc acgtgcgacc cgggccgcac
     7801 ggggccgcgg ccgtcgtgca gcttgccgtc gatctcgatc gactccatcg cccggcacat
     7861 gatcttgtcc tccgagcggt agagcacgac gtcgccgccg aactcgcggc agacgacgtg
     7921 gttctgccac tgcggaccca gcacgcacga ttcgcccatc agcacgaccg cgtcggcata
     7981 ggggagcgtg cggtgcgggc tgagcagctc cagtcgggcc gagctggaga gcacgtgcgg
     8041 cttgcggaac cgcattttca cccgctcgcc gagcgtgaac tcgtcgccgt cggagagcag
     8101 ggccggctcg gtgaccgcct tgccactcag gacaactttg ccgccgagcg gttcgaggat
     8161 gtagccttct ttatgccgca cgagcttcac gtgcttgcgg gagatgtcgg cctggatcgg
     8221 cacatcgacc tttccgccgg gcacggcctg gccaatgatg atctcatcgc ccaggcagac
     8281 gagataaccg ccgacggcgt cgatccacaa catgaagcgg ggcgaccgct tgacgggcac
     8341 gtcggcgccg ccgaccgtga cggggccgga aatcggcgtg ccggtccagt gcttggtttc
     8401 gccgagtcgg cgggaatcgc cgatctgctc gccgacttcg ccccaggccc gcttgcgggc
     8461 gtcgcgggcg acgcggtttt cgggtgagat ttccagcagc tccgtcgcca acgacaagac
     8521 cttggtccaa tcgctggcgg ccagcgccgc gtgcaattgc tcggtgagcg tcttggagcg
     8581 ctcctgccgc tcgcggcagc gctcgcgctg ggcctcgatc agtttcaagt cgggccgcag
     8641 cgcttgggcc gcggccagtt gctcgtcggc ctcggagaat ttgcctttct ggctgagctt
     8701 gcgggccgag tcgagccgcc gcgtcacttc cagcagcgtc ttcagcggca ggcccggcac
     8761 gccgcgtttt tcgagcgatt cgatgcggga ggtcgccccg ggataatcgc tggccgtaag
     8821 gtgctgcacg atatcgccca ccgcgacgtc ggcgacggcc tgctgcaggc gttgccagct
     8881 gccggtttcg ccggcgaggc tgcgggcttc atccagatct cgccagccgg ccgtgaaatc
     8941 gcccgaggcg gcccgctcca aggctcggcg tgcgaactgc tcgccgatct gcgccaggag
     9001 ctgctggacg ggaagaaact gcttgagctg cgggtcgctg gccaggcgcg ccgcttcctc
     9061 caatcgccct tgctctaagg cgacctgtgc ttcccgcaac ttgaatcgcc acggaggaaa
     9121 catgatcacg cgtgcccgct ccctaacagc ttactcgttc tcgaggcgat cggcatcctt
     9181 cgcggctagg ggctcacgct gtacgccggc tgccggatcg gcttggcagg cggcgcgtcg
     9241 ccggcgcatt tcaagtgccg gggcgtccga tgccccaggg cctgacagag ctgaatgatt
     9301 tcttccggcg tgaagccgcg ggcgatcata tcgcggatca gcgccgtcga ttgcgactgc
     9361 ttccagcaca ggtacgtgta gtggcagacg atccacagcg cgggcaggac gaggagaata
     9421 gccatggcag ataaccccat gatcacgtcg tcgttcatgg aatgactcca cagtgcggcg
     9481 acctactcaa gtattcgccg cataggccgg atacttgatc ggcttggcgg gcggcacgtt
     9541 ggaagttccg ctcttcgact tgccgcggcg ggccgaaacg atttctacga tctcctgcac
     9601 cgtgtaaccg cgggcgatca tgtcgcgctt caggcgggtc tcttgccagg tcttgaacgc
     9661 ggcatagatg tagtgcagca ccagccacag aatgcaggcc agaaagggca ggccgaagac
     9721 gccaaatatg ccggccaggg cgacgaccat ttcttcttca cgcatggacg ggactccgca
     9781 agcggctgaa aagaagaggc caaaaaacga gaagccccgc ttcaggcgca gtcgaagcgg
     9841 agcttctgaa agtgcggtcc caagcggagc ttggaaccga gttacgtttt gtggaaggcg
     9901 tttactcggc ttccagcttc acggcgacgg tggcgtcggc tttcttcgag gcttcgaagt
     9961 aggccgtgac ctggtcggcg atgttgccgc tggtctgctc ttcgtcgagg ttgatcgcgc
    10021 ccaccgacga cgtgtcggcg ctgaccagct tctcggcgac gtcgatccgc gtgctgatat
    10081 ccttcatca
//
LOCUS       DASRJB010000307         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_370933, whole genome shotgun sequence.
ACCESSION   DASRJB010000307 DASRJB010000000
VERSION     DASRJB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370933"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFB80_08305"
     CDS             <1..779
                     /locus_tag="VFB80_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN33807.1"
                     /translation="DLHLFSSSVNAITDVLDEQGLVYDVERKRVRKNNEERVFTHIHV
                     QDRFPLELTIYPADKAHYVFKSSITGKPIERASIRELESLLSREYPALDIEAAVSSAA
                     EQLDPFQVFQSLLLPLENVKQNLKYHPEGDALYHSLQVFDHARDASPYDEEFLLAALL
                     HDVGKAIDPEDHVAAGLEALDGFITDRTRWFIEHHMEAQQDLDQTLGQRARRRLRESP
                     DYDDLITLARCDRAGRQIGAQAPELEEALDYLRELARAFG"
     gene            882..1331
                     /locus_tag="VFB80_08310"
     CDS             882..1331
                     /locus_tag="VFB80_08310"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HZN33808.1"
                     /translation="MMQIWVDADACPGEIKDLLFRAAQRLKIKLTLVANQSLRIPRSE
                     FIHTLLVPDGMNIADRRIVELVAPGDLVVTADIPLAADVVAKGAEAIDPRGGLYTEAN
                     IGERLGMRNMLDQLRGGGQLSRGPATFGSKDRQAFANQLDRWLVKHQ"
     gene            1357..2013
                     /locus_tag="VFB80_08315"
     CDS             1357..2013
                     /locus_tag="VFB80_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZN33809.1"
                     /translation="MDPHTRSLAEEIVNSVTHGIGLILSLAGGWLLLMRVHSQPDAWR
                     LVGCGVFAIALVAVYAASTLSHAVARPAWRQAFRILDQGCIYLLIVGTYTPFALEYLR
                     FGWWWPFLLLMWTAALVGFLSKLLFLHRIDAATIWSYLLLGWLPIIPAWAYLNLVPLG
                     ALAWILVGGLCYTAGTAFLVLDHRPYQFHAIWHLFVIAGSVCHYIGVFLFVACAPLHV
                     "
     gene            complement(2141..3727)
                     /locus_tag="VFB80_08320"
     CDS             complement(2141..3727)
                     /locus_tag="VFB80_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN33810.1"
                     /translation="MTQQSQAVPTLADEGVPASQLLRQLVVNIATQSWLASMALHMTL
                     LIVLALVLGTIHVAATIGAAPAFEVPEEPKSLEPEIEHFVVGEAPLEPTILNAQTLTS
                     EPPAGLEDALAESFLFEPAGGTVNPGQLQSTGMSFASDLGLGPALREGAGSGGLGLGM
                     EAGQGGAQAGYSVRTGDARSKLRGLGATIPSERAVAAALNWLARHQSPDGRWSLDHTP
                     RCKSGSCSGPGQQRADAAATALGLLPFLAAGQTHESVGPYRKNIAAGIDWLIKNQQRT
                     GDLSSGGSQMYSHGLATIALCEAYGMTHDSRVGYAAQAALDFIISGQNKEGAWRYTHG
                     TESSDTSVFGWQVMALKSGQMAGLKVQPAALDGCRQYLRKVAQGQYQSEFSYAPGGGP
                     TFTMTSVGLLTSQYLGARRDDPVIVGGLDYLSRHKPSAQQRNSYYWYYATQVMHNVPG
                     PQWDDWNRAMRRLLIETQEQSGCQSGSWDPSRPEPDPWGNQGGRLMVTSLSCLTLEVY
                     YRYLPLYKIDGDGAELKGLP"
     gene            complement(3859..4092)
                     /locus_tag="VFB80_08325"
     CDS             complement(3859..4092)
                     /locus_tag="VFB80_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33811.1"
                     /translation="MKFTVRDLLWLAAVLFFAAGWYIDRRVATWERRVAQAKLMLAES
                     KLQELGWRLRSSSNGREFGVEPLRKYEPADSAR"
BASE COUNT          717 a         1433 c         1372 g          827 t
ORIGIN      
        1 tcgacctgca cctgttctcg agcagcgtca acgcgatcac ggacgtgctc gacgagcagg
       61 gcctggttta cgacgtcgag cggaagcgcg tccgcaagaa taacgaggag cgggtcttca
      121 cgcacatcca cgtccaggac cgatttccgt tggagctgac gatctatccg gccgacaagg
      181 cccactacgt cttcaagagc tcgatcaccg gcaaaccgat cgagcgggcc agcatccgcg
      241 agctggagtc gctgctgtcg cgcgagtatc cggcgctgga tatcgaggca gcggtcagca
      301 gcgcagccga gcagctcgac ccgttccagg tctttcagag cctgctcttg ccgctggaaa
      361 acgtgaagca gaacctcaag taccacccgg agggtgacgc gctgtaccac agcctgcagg
      421 tcttcgacca cgcccgcgac gcctcgccct acgacgagga gtttttgttg gcggccctgc
      481 tgcacgacgt gggcaaggcg atcgacccgg aggaccacgt cgccgccggc ctggaagcgc
      541 tcgacgggtt catcaccgac cgcacgcggt ggttcatcga gcaccatatg gaggcccagc
      601 aggacctcga ccagacgctc ggccagcggg cccgccgccg gttgcgcgaa tcgcccgatt
      661 acgacgacct gatcaccctc gcccgctgcg accgcgccgg ccgccagatc ggcgcccagg
      721 cccccgagct cgaagaagct ctcgactacc ttcgggaact cgcccgagcc ttcggttaat
      781 agcagtcctc acgctccgcg tgagaaacgt ggtgtgggct ctggcctgcc caggcgcttg
      841 cccgcgagct gccgatgggg tcaagtcgag actgagaggg aatgatgcag atttgggtcg
      901 atgccgatgc ctgtcctggc gaaatcaagg acctgctgtt tcgggccgct cagcggctga
      961 agatcaagct gacgctcgtc gcgaatcagt ccctgcggat tccgcggtcc gagttcatcc
     1021 acacgctgct cgtaccggac ggcatgaaca tcgcggatcg acgaatcgtc gagttggttg
     1081 caccagggga tttggtagtt accgccgata ttccgctcgc tgccgacgtg gtggccaagg
     1141 gcgccgaggc gatcgatccg cgcggtggac tttacaccga ggccaacatc ggtgagcggc
     1201 tggggatgcg gaacatgctc gaccagctcc gcggcggcgg ccagttgagc cgcgggccag
     1261 ccaccttcgg ctccaaagac cgccaggcct tcgccaatca gctcgatcgc tggctggtca
     1321 aacaccaata ggctcgctct ttgccgggct cgtggaatgg atcctcacac acgttcgctc
     1381 gcggaagaaa tcgtcaacag cgtgacgcat ggcatcggcc tgatcttgag cctggccggc
     1441 ggctggctcc tgctcatgcg agtccattca cagccggacg cgtggcgtct ggtggggtgc
     1501 ggcgtttttg cgatcgccct cgtggcggtg tacgcagctt ccacgttatc gcacgccgtc
     1561 gcgcggccgg cctggcggca ggccttccga attctcgatc aaggttgcat ctacctgctc
     1621 atcgtcggaa cgtacacgcc ctttgccttg gagtacttgc gcttcggctg gtggtggccg
     1681 ttcctgttgc taatgtggac ggccgccttg gtaggctttc tttccaagtt gctgttcctg
     1741 caccgtatcg acgcggcgac gatctggtcg tacctgctgc tcggctggct gccgattatt
     1801 cctgcctggg cgtacctgaa tttagttccc ctcggcgcct tagcttggat tctggtcggc
     1861 ggactgtgct acaccgccgg caccgcgttt ctggttctcg accatcggcc ctatcagttc
     1921 cacgcgatct ggcacctctt cgtcatcgcc ggcagcgtct gccactacat cggcgtattc
     1981 ctcttcgtcg cctgcgcccc gcttcacgta tagctatgcg ggcgcaggcg atcacaaccc
     2041 gtcctaaact cgttcctgct actggctgtg gttcgctgaa tgatcgacgt cgctctgccg
     2101 ctcaacgcgc acctttacct caaattcttg atagggtccg tcaaggcaaa cccttgagtt
     2161 ctgctccatc gccgtcgatc ttgtacagcg gcaagtagcg gtaatacacc tccagcgtca
     2221 ggcacgagag gctggtcacc atcaggcggc cgccttgatt gccccaggga tcgggctcgg
     2281 gccggctggg atcccagctg ccggactggc agcccgattg ctcctgcgtc tcgatcagca
     2341 gccgccgcat ggcgcggttc cagtcgtccc actgcgggcc gggcacgttg tgcatgactt
     2401 gcgtcgcgta gtaccagtag tagctgtttc gctgctgggc cgacggcttg tggcgcgaga
     2461 gataatcgag gccgccaacg attactgggt cgtcgcggcg ggctcccaga tattgcgacg
     2521 tcaacagtcc gacggaggtc atggtaaagg tcggacctcc gccgggggca tagctgaact
     2581 cgctctgata ctgcccctgc gcgactttgc ggaggtattg ccggcagccg tcgagcgcgg
     2641 ccggctggac cttgaggccg gccatctggc cgctcttgag ggccatcacc tgccagccga
     2701 agaccgacgt gtcggagctt tctgtgccgt gcgtgtaccg ccaagcgccc tccttgtttt
     2761 gcccggagat gatgaagtcc agcgcggcct gggcggcgta gccgacgcgg gagtcgtgcg
     2821 tcatgccata ggcttcgcag agggcgatcg tcgccaggcc gtgcgaatac atctgcgatc
     2881 cgccgctgga gagatcgccg gtgcgctgct gattcttgat cagccagtcg atgcccgccg
     2941 cgatgttctt gcgatagggc ccgacgcttt cgtgcgtctg gccggcggcc agaaacggca
     3001 agagtccgag cgccgtcgcg gctgcatccg cacgctgctg gccagggccg gagcacgagc
     3061 cgctcttgca gcgcggcgtg tgatccaggc tccaacggcc atccggactt tggtggcgcg
     3121 cgagccagtt aagggccgcg gcgacagccc gctccgaggg gatcgtcgcg cccaagcccc
     3181 tgagcttcga ccgagcgtcg cccgttcgga cgctgtaccc agcctgcgcg ccgccctggc
     3241 cggcttccat gcccagtccc aagccgccgc tgccagcacc ctcgcgcagc gccggcccga
     3301 ggcctaagtc actcgcaaat gacatcccgg ttgactgcag ctgaccggga ttgacggttc
     3361 cgccagccgg ttcgaacaaa aaggactcgg ccagggcgtc ttccaggccc gccgggggct
     3421 cggaggtcaa cgtctgggcg ttcaagatcg tgggttccag cggcgcttcg ccgacgacga
     3481 agtgctcgat ttccggctcc agcgattttg gctcctccgg cacctcaaac gccggagcag
     3541 cgccgatcgt ggcggccacg tgaatcgttc ccagcaccag cgccaggacg atgagcagcg
     3601 tcatgtgcag ggccatgctg gccagccacg actgcgtggc gatgttgacg accagttgcc
     3661 gtagcagctg gctggcgggc acaccctcat cggctagcgt cggcacggcc tgtgactgct
     3721 gagtcatggg ttcctccagc gcagagatcg atccgatgcg gcaccattgg ccagccgctg
     3781 cggggctttg ccgggcgaga aaaggggccg gcgattgttt cggtccgccc tgcgcgaagt
     3841 attcccgcca gcttccaact acctggccga atcggcgggc tcgtacttgc gcagcggctc
     3901 gacgccgaat tcccgtccgt tagaactgct cctcagccgc cagcccaatt cctggagctt
     3961 agattcggcg agcatcagct tcgcctgagc aactcgccgc tcccaggtag ccactcggcg
     4021 atcgatgtac cagcccgcgg caaagaacag cacggccgcc agccacagca aatcccggac
     4081 ggtgaatttc atgccggcat tctagtcgcg ctccggctga aggtgctcag ccggcgctgc
     4141 ggaccgagag cgtatcggcc tcgttcgccg ccgcggtcac gagcgggtgg aggtccgggc
     4201 agatcgcggc gatgttggct aggtcggcca gcagcttgcg ctgaacggtt gaaggcagct
     4261 cccggtattg ggcgatgatg tttgtcagcc gctcgtcgtt cgtcggcccc tcggcgtccg
     4321 ctagttgatt gaccccaagc cagaactat
//
LOCUS       DASRJB010000308         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1215246, whole genome shotgun sequence.
ACCESSION   DASRJB010000308 DASRJB010000000
VERSION     DASRJB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215246"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..980
                     /locus_tag="VFB80_08330"
     CDS             18..980
                     /locus_tag="VFB80_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33812.1"
                     /translation="MRRLLLLALFVAALVPLVALAQTKAPKTVKRAQPPKFSKTDNFY
                     SDAFKEGLVGERPANLAKAAAAAAPGGNSGSSAPPSGSGPGVTGAGWAALISATTIED
                     EIKATKLLVDGGVTTPSDFAGKGYKAARRNFTVLAMLFGIAGEYDSDVRWKKDAPAAR
                     DAFARTAANAKVGTQQVWQEARLRKDELGDLINGGTPFAGKEAEAKAPWKDVAGRAPL
                     MQFLEQAWDPRLKPALADKGQFTANADRVLRDAELLAAIGAVLAKDGMPDADSDEYKS
                     FCARLRDGAKSIADAVKTKNYEQAARASAAIGKACAECHENYRS"
     gene            complement(1033..2112)
                     /locus_tag="VFB80_08335"
     CDS             complement(1033..2112)
                     /locus_tag="VFB80_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN33813.1"
                     /translation="MHATPLNRRTFLQAAGAAAALAGTAAAQQAAAGPANTLGISYSS
                     YSVRWQAATADGQLPRMTGAVDFLRHAKKIGAAGVQTVVGEWDERQAREVRELIDREG
                     LFFEGQVGLPRVPADAERFDKQIQAAKAAGASIVRAVCLGGRRYETFATDAEFAAFVQ
                     QSRAKLRLAEPIVKRHRVRLAIENHKDWRIGEMLDLLKQLASEWVGINLDIGNSIALL
                     EDPLEVVEAYAPLTYTTHFKDMGVRAYDDGFLLSEVPLGEGYLDLAKIIAVCRQANPA
                     VRFNLEMLTRDPLQVPCLTERYWVTMPAVSGRDLARTLAAVRSKSSAKPLPAVAGLSR
                     DERIRYEEANVVQSLEFARKKLGLA"
     gene            2276..3091
                     /locus_tag="VFB80_08340"
     CDS             2276..3091
                     /locus_tag="VFB80_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33814.1"
                     /translation="MEILLGQHEAFFLTGLFSLGGSLMWLVWAAIHLGVIIHVLRTGR
                     PYWWIYVVFFFPYVGALAYLFFEVRPSVGKFDYRSLLWRLKSPQQRIAIREGILEDSN
                     TVRNRLALADELHFARQFDRECTVLSEGLRGAFKDDATLLMRLSQAHLEAGRPAEAEQ
                     VLAKTVPEKSPDQQLQFSLLTARVAARCGRPAEAEKIFQELMAKKKSEGPRFYYAEYL
                     MHSGQRDQATLILRDILLQYRRGTPVWRFVERRWFYAAKALLKAKPPAATMKG"
     gene            3088..4044
                     /locus_tag="VFB80_08345"
     CDS             3088..4044
                     /locus_tag="VFB80_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZN33815.1"
                     /translation="MNPRIVYDRRVNLGFPGSQRLHPFDLRKYARAWAVLRKQLGSRL
                     AALHLPTPAPASDDDLRLVHSAEYLQSLQSSAVIAQAIEVPALRRAPLWLLDRFLLQP
                     MRWAAAGSVMAARAALECGLAFNLGGGFHHAKPAGGEGFSIYNDIALIVRKLTREGRL
                     AEGSRVVCVDLDAHLGNGVAWCFHDDPRVFLFDMHNALIYPMFDVQARERIDCPIGLA
                     PGTGGGEYLEQLRSRLPPFLDSISRSQRPALAIYNAGTDVLAGDELGGLSLTPDDVLA
                     RDLFVLNLLRDRQIPAVMLTSGGYTQQSYQVIARTIAAAASH"
     gene            4177..>4715
                     /locus_tag="VFB80_08350"
     CDS             4177..>4715
                     /locus_tag="VFB80_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33816.1"
                     /translation="MRQSAPRLRGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRT
                     QCTNQIKQIALGVHNFENTHGTVPPAVGFSPTGGGSTTGGFGAGWGWLCFMLPYIEQK
                     ALYDRIDFNINVCCKSMAPLHNAIVPGLTCPSDPLGNKLLAARGLPNSTCNDASGSAT
                     TLQAVTGGNAQCISSRPSS"
BASE COUNT          757 a         1632 c         1524 g          802 t
ORIGIN      
        1 tctcccggag ttgatccatg cgccgtttgt tgctgctggc cctgtttgtc gccgcgctcg
       61 tcccgctggt tgctctcgcc caaacgaagg cccccaagac ggtcaagcgg gcccagccgc
      121 cgaagttcag caagacggac aacttttatt ccgacgcgtt caaggaaggc ctggtcggcg
      181 agcggccggc gaatctggct aaggcggccg cggcggccgc gcctggcggc aattcgggct
      241 cgagcgcgcc accatccggc agcggccccg gcgtgaccgg cgccggctgg gcggcgctga
      301 tctcggcgac gacgatcgag gacgaaatca aggcgaccaa gctgctggtc gatggcggtg
      361 tcacgacgcc cagcgatttc gcgggcaagg gatacaaggc ggcccgccgc aacttcacgg
      421 tgctggcgat gctgttcggc atcgccggcg agtacgacag cgacgtgcgg tggaagaagg
      481 acgccccggc cgcccgcgac gccttcgctc ggactgccgc taacgccaag gtcggcacgc
      541 agcaggtctg gcaggaggcc aggctccgca aggacgagct gggggacctg atcaacggcg
      601 gaacgccctt cgccggcaaa gaagccgagg ccaaggcgcc ctggaaggac gtcgccggcc
      661 gcgccccctt gatgcagttc ctcgagcagg cctgggatcc acggctgaag cccgcgctcg
      721 ccgataaggg acaattcacc gccaacgccg accgcgtgct ccgcgacgcc gagctgctgg
      781 cggcgatcgg cgcggtgctg gccaaggacg gcatgcccga cgccgactcg gacgagtaca
      841 agtccttctg cgctcgcctg cgcgacgggg ccaaatcgat tgccgacgcc gtaaagacga
      901 agaactacga gcaagccgcc agggccagcg ctgcgatcgg caaggcctgc gccgagtgcc
      961 acgaaaacta ccgctcgtag ccgacgctgt cagcgtcggc gcgtcggcag cggacgacgc
     1021 tggcagcgtc ggctacgcca atcccaactt cttcctggcg aattccagcg actgcaccac
     1081 gttggcctct tcgtagcgaa tccgctcgtc gcggctgagt ccggcgacgg cggggagcgg
     1141 cttggccgac gacttgctcc gtacggcggc cagtgttcgg gccagatcgc gccccgacac
     1201 ggctggcatc gtcacccagt agcgctcggt caggcagggg acctgcagcg gatcgcgggt
     1261 cagcatttcc aggttgaagc ggaccgcggg gttcgcctgg cggcagacgg cgatgatctt
     1321 cgccagatcg agatagccct cgccgagcgg cacctccgac agcaaaaagc cgtcgtcgta
     1381 ggcccgcacg cccatgtcct tgaagtgcgt cgtatacgtc agcggcgcgt aggcctccac
     1441 gacctccagc gggtcttcga gcaacgcgat gctgttgccg atgtcgaggt tgatgcccac
     1501 ccactcgctc gccaactgct tgagcaggtc gagcatttct ccgattcgcc agtccttgtg
     1561 attctcgatc gccagccgca cgcggtgcct cttgacgatc ggctcggcga gccggagctt
     1621 ggcgcgagac tgctgcacga aggcggcgaa ctcggcgtcg gtggcaaagg tctcgtaccg
     1681 ccggccgccc aggcaaaccg cccgcacgat cgaggccccc gccgccttgg cggcttgaat
     1741 ctgtttgtcg aaccgctcgg cgtcggccgg gacgcggggc aggccgacct ggccctcgaa
     1801 gaacagtcct tcgcgatcga tcagctcccg gacctcgcga gcctgccgct cgtcccactc
     1861 gccgacgacc gtctgcactc ccgccgcgcc gatttttttg gcgtgccgga gaaagtccac
     1921 cgcgccggtc atccgcggta actggccgtc agccgtggcc gcctgccagc ggaccgaata
     1981 ggacgaatag ctgatcccca gcgtgttggc cggcccggcg gcggcctgct gggcggctgc
     2041 cgtgccggcg agcgccgcgg cagcccccgc ggcctgcaaa aacgtgcggc gattgagcgg
     2101 tgtcgcgtgc atggtcccct cccgccgcaa tcttccgccc cggcagcggc cgttgtcaat
     2161 cttccggccc tgtgtagtcc gctcgctccg cgagcggacc gcctcacgcg gagcgtgagg
     2221 gctactttag gcagcatcgt tgagccaatc gccgccgcat cgtaaattgc ctgccgtgga
     2281 aatcctcctg ggccagcacg aagcgttctt cctgaccggg ctgttcagcc tcggcggctc
     2341 gctcatgtgg ctggtttggg ccgcgatcca tctcggcgtg atcatccatg tgctgcgcac
     2401 tgggcggccg tactggtgga tctacgtcgt cttcttcttt ccgtatgtgg gggcgctcgc
     2461 ctatctgttc tttgaggtcc gaccctcggt cggcaagttc gactatcggt cgctgctctg
     2521 gcggctgaag agcccgcagc agcggattgc catccgcgag ggcattctgg aagattcgaa
     2581 caccgtgcgc aaccgcctgg cactggcgga cgagctgcac ttcgcccgcc agttcgaccg
     2641 cgagtgcacc gtgctcagcg agggactgcg cggcgcgttc aaggacgacg ccacactgct
     2701 gatgcggctg tcgcaggccc acctggaagc cggccgcccc gccgaagccg agcaggtgct
     2761 ggccaaaacg gtgcccgaaa aatcgcccga ccagcagctg cagttctcgc tgctgacggc
     2821 ccgcgtcgcc gcccgctgcg gccggcccgc cgaagcggaa aagatctttc aggaattgat
     2881 ggcgaagaaa aagagcgagg gcccgcgctt ttactacgcc gagtacctga tgcacagcgg
     2941 ccagcgcgac caggcgacgc taatcctccg cgacattctg ctccagtacc gtcgcggcac
     3001 gcccgtctgg cggttcgtcg agcggcgctg gttctatgcc gccaaggcgc tgctcaaggc
     3061 caagcccccc gccgctacga tgaaggggtg aacccgcgaa tcgtctacga ccgccgcgtc
     3121 aacttgggtt ttcccggctc ccagcggctg cacccgttcg acctgcggaa gtacgcgcgg
     3181 gcctgggctg tcctgcggaa gcagctcggc agccggctgg ccgcgctgca tctgccgacg
     3241 cccgctccgg cgagcgacga cgacttgcgg ctggtgcact cggccgagta cctccagtcg
     3301 ctccagagct cggccgtcat tgcgcaggcg atcgaagttc cggcgctgcg gcgggccccc
     3361 ttgtggctgc tcgaccgatt tctgcttcag ccgatgcgct gggccgcggc cggctcggtc
     3421 atggccgccc gcgcggcgct ggagtgcggc ctggcgttca acctgggcgg cggttttcat
     3481 cacgccaagc ccgccggcgg cgagggtttt tcgatctaca acgacatcgc cttgattgtg
     3541 cggaagctga cgcgcgaagg ccgtttggcc gaaggcagcc gcgtggtctg cgtcgatctc
     3601 gacgcgcacc tgggcaacgg cgtggcctgg tgctttcacg acgatccgcg ggtctttctg
     3661 ttcgacatgc acaacgcctt gatctatccg atgttcgacg tccaggcccg cgagcgaatc
     3721 gattgcccca tcggccttgc gccgggaacc ggcggcggcg agtacctgga gcaactccgc
     3781 agccgcctgc cgccgtttct cgactccatc agccgctccc agcggccggc gctggcgatc
     3841 tacaacgcgg gcaccgacgt gctggctggc gacgagttgg gcggtctttc gctcacgccc
     3901 gacgacgtgc tggctcgcga cctgttcgtg ctgaatttgc ttcgcgatcg ccagattccc
     3961 gccgtcatgc tcaccagcgg cggttacacc cagcagagct accaggtgat tgcccggacg
     4021 atagccgcgg cggcgtccca ctagcgacaa aaaccccatt tttctgcttg gcatcgaagc
     4081 agggctgcac taagatgaga cgccgttgaa tcttacgcga attcgcgacg ctgatcctcg
     4141 ctggggctcg cgactttacc cgaaatgagt agtgccatgc gccagtccgc gccacgcttg
     4201 cgtggcttta cgctcgtcga actgctcgtc gtcatcgcca tcatcggcgt gctcgtagcg
     4261 ctgctgctgc ccgcggttca ggccgcccgc gaagcggccc gccgcacgca atgcaccaac
     4321 cagatcaagc aaatcgcgct gggcgtgcac aatttcgaaa acacgcacgg caccgtcccg
     4381 ccggccgtgg gcttcagtcc cacgggcggc ggcagcacca cgggcggctt cggcgcgggc
     4441 tggggctggc tgtgcttcat gctgccctac atcgagcaga aggccctgta cgatcgaatc
     4501 gacttcaata tcaacgtttg ctgcaagagc atggccccgc tgcacaatgc cattgttccc
     4561 ggattgacct gccccagcga cccgctcggc aataagctgc tcgcggctcg gggtcttccg
     4621 aattccacgt gtaacgatgc cagcggctcc gccaccacct tgcaggcggt cacgggcggc
     4681 aacgcccagt gcatctcgtc gcggccgtcg agcta
//
LOCUS       DASRJB010000309         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2345801, whole genome shotgun sequence.
ACCESSION   DASRJB010000309 DASRJB010000000
VERSION     DASRJB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345801"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VFB80_08355"
     CDS             complement(<1..2079)
                     /locus_tag="VFB80_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZN33817.1"
                     /translation="MPPTRAEAKPAPAATYFPLVLIVCALAGGIALDRLYPLAAALWM
                     GVAAAAVTLWLICWLGHCNRLASVLLLAGFFAVGGARHHQYWRLFGEDEIGRSVDEIL
                     RPAVIEAEALTSPRWVPAPPPTPLRTIPQGEHCELIVNVTAIRDGRTFRPASGLAELQ
                     VGGNLRGIRAGDRLRIMAQAGRPAAPLNPGEFDFAAHLRAERQLCRLFAEFPESVERL
                     SPGSVFSPRRWLAAVRGGGSEILRQNIDPNRATLASAVLLGAREQLDADRNEGYLVTG
                     TIHVLSISGLHVGILAAGFYVLFRTGLLPRRFMLAATILLTIAYACLTDLQPPVVRAT
                     IIVIVACLALWHDRAALGFNTLAAAGIVVLALNPASLFQAGPQLSFLAVATMIAFQPL
                     LMPQPITDPLDRLIARSRPWPIRLASSTGGILWRVWLTGALIWLVSTPLVWKQYNLIS
                     PIAQILNFLMWLPVSLAMYSGLGTLVLGPFIPLGGKWLGVSCDKCLWLLEFFIAQGRE
                     VRGAYFWHPAPPGWLIGVFYGLLAVFVAFPALRPRRHWLAAIAAAWVAVALATSGTGS
                     SMLAQVRPRPLGCSFVAVGHGVSVLVELPDGKNLLYDAGRLGSPLAGVRPVSAVLWSR
                     GITHLDAVVVSHADADHFNALPDLLERFDVGVIYVSPVMFERRQPAVEELRRSIALAG
                     VPLQEIHGGQR"
     gene            complement(2089..>2567)
                     /locus_tag="VFB80_08360"
     CDS             complement(2089..>2567)
                     /locus_tag="VFB80_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZN33818.1"
                     /translation="MIAFQPLLMPQPITDPLDRLIARSRPWPIRLARSTGGILWRVWL
                     TGALIWLVSTPLVWKQYNLISPIALVLNFLMWLPVSLAMYSGLGTLVFGPFISLGGGG
                     KWLGVSCDKCLWLLEQLIAWGREIPYAYLGHPAPPSWLFALGRCGIAKVERACGMQ"
BASE COUNT          518 a          885 c          851 g          313 t
ORIGIN      
        1 ccgttggccg ccatgaatct cttgcagcgg cacgccggcc agcgcaatgg accttcgcaa
       61 ttcttccacc gccggctgcc ggcgctcgaa catcaccgga gagacgtaga tcacgccgac
      121 atcgaaacgc tcgagcaggt cgggcagggc gttgaaatgg tcggcgtcgg catgcgagac
      181 gaccaccgcg tcgaggtgcg tgattccgcg cgaccagagc accgccgaga ccggccgcac
      241 gcccgccagc ggcgatccga gccggcccgc gtcgtagagc aggttcttgc cgtcgggcaa
      301 ctccacgagc acgctgacgc cgtggcccac ggcgacgaag ctgcagccca gcggccgcgg
      361 ccggacctgc gcgagcatgc tcgagcctgt gccggacgtc gccagcgcaa cggcgaccca
      421 cgccgcggca atcgccgcca gccaatgccg ccggggacgc agcgctggaa aggcgacgaa
      481 gaccgccagc agcccataga acacgccgat caaccagccg ggcggcgcgg ggtgccagaa
      541 atacgcgccg cgaacttcac gcccctgcgc gatgaaaaac tccagcagcc acaggcactt
      601 gtcgcaactg actcccagcc acttgccgcc gagcgggatg aaggggccga gcacgagcgt
      661 ccccaggccg gaatacatag ccagcgatac cggcagccac atcaaaaaat tcaaaatctg
      721 cgcgatcggc gaaatgaggt tgtattgctt ccaaaccagc ggcgtcgaaa cgagccagat
      781 cagggccccc gtcagccaga cgcgccacag aatgccgccg gtactgctgg ccagccggat
      841 cggccacggc cgcgagcggg cgatcagacg atcgagcgga tcggtgatcg gctgcggcat
      901 gagcagcggc tgaaaggcga tcatcgtcgc cacggcgaga aacgagagct gcggtccggc
      961 ctgaaacagg ctcgccgggt tgagggccag caccacgatg ccggctgccg cgagcgtgtt
     1021 gaagcccagc gccgcgcgat cgtgccacag cgccaggcag gcgacgatga cgatgatcgt
     1081 ggcccgcacg accggcggct gcagatcggt caggcaggca taggcaatgg tgagcaggat
     1141 ggtcgcggcc agcatgaacc gccgcggcaa caggcccgtg cggaagagca cgtagaagcc
     1201 ggccgcgagg attccgacgt gcaggcccga gatcgagagc acgtggatcg tgccggtgac
     1261 gagatagccc tcgttgcggt cggcgtcgag ttgttcccgc gcgcccagca gcaccgcgga
     1321 ggccagcgtc gcccggttgg gatcgatgtt ctgccgcaag atctcgctgc cgccgccgcg
     1381 aacggcagcc agccaccgcc gcggcgaaaa gacgctgcca ggcgacagtc gctcaaccga
     1441 ttccgggaac tcggcaaaca accggcacag ctgccgctcc gcccgcaggt gggcggcgaa
     1501 atcgaattcg ccgggattca gcggcgcggc gggccgcccg gcttgcgcca tgatccgcag
     1561 ccggtcgccg gcccgaatgc cccttaggtt gccgccgact tgcaattcgg ccagcccgct
     1621 ggcgggccga aacgtgcggc cgtctcggat cgccgtgacg ttgacgatca gctcgcagtg
     1681 ctcgccctga ggaatcgtcc gcagcggcgt cggcggcggt gcaggcaccc accgcggcga
     1741 cgtgagcgcc tccgcctcaa tcaccgccgg ccgcagaatc tcatccaccg accgaccgat
     1801 ctcatcctcg ccaaacagcc gccagtactg gtggtgacgt gctccgccca ccgcgaagaa
     1861 tccggcgagc agcaacactg aagccagccg gttgcaatgg cccagccagc aaatcagcca
     1921 caaggtcaca gccgccgcgg caactcccat ccaaagggcc gccgccagcg gatagagccg
     1981 atcgagcgcg atcccacccg ccagcgcgca gacaatcagc acgagcggga aataggtcgc
     2041 cgctggcgct ggcttcgctt cggccctggt cgggggcatg gcgagagttt actgcatgcc
     2101 acaggcccgc tctaccttgg caatgccgca gcgacccagc gcgaacagcc agctgggcgg
     2161 cgcggggtgc ccgagatagg catagggaat ttcgcggccc caggcgatga gctgctccag
     2221 cagccacagg cacttgtcgc aactgactcc cagccacttg ccgccgccgc cgagcgagat
     2281 gaaggggccg aacacgagcg tccccaggcc ggagtacatg gccagcgata ccggcagcca
     2341 catcaagaag ttgaggacga gcgcgattgg cgaaatgagg ttgtattgct tccaaaccag
     2401 cggcgtcgaa acgagccaga tcagcgcccc ggtcagccag acgcgccaca gaatgccgcc
     2461 ggtactgcgg gccagccgga tcggccacgg ccgcgagcgg gcgatcagac gatcgagcgg
     2521 atcggtgatc ggctgcggca tgagcagcgg ctgaaaggcg atcatcg
//
LOCUS       DASRJB010000310         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1500173, whole genome shotgun sequence.
ACCESSION   DASRJB010000310 DASRJB010000000
VERSION     DASRJB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500173"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1560)
                     /locus_tag="VFB80_08365"
     CDS             complement(85..1560)
                     /locus_tag="VFB80_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN33819.1"
                     /translation="MCHRRLLLGMLAAACLSTAAAARDDSPLADAAERSDRARVESLL
                     SDKAAVNQAQPDGMTALHWAAHHDDVELARRLLAAGADVRLENRYGVAPLPLACENGN
                     GELVELLLKSGADPNTKLRGGETALMTAARTGRLAPVKALLAAGADVNAKERKGQTAI
                     MWAAAEGHVEVAQALLDAKAEFRQPLASGFTPLLFAVRAGHADVVQLLLTAGADVTEA
                     AASRRLGGGGNGGISPLLVAVENGHFELALSLVKAGADPNDQRSGFAPLHALVAVRKP
                     NRGEGDDGDPAPRGSGSVSSPDFVRELVKLGADVNLRLKRGESGGGQLGKSGATPLLL
                     AAFRDDVPLIKLLVELGADPLIANADGCTPLLAAAGVGTRFPGEEAGTEAEALETVAL
                     LLELGSDINAVDRNGESAMHGAAYKSMPRMVQLLADKGAKIDTWNKTNKHGWTPLHIA
                     LGYRFGNFKPAAETIAAMHKVMLAAGVAPTNDPPKSGRKDY"
     gene            complement(1573..2913)
                     /locus_tag="VFB80_08370"
     CDS             complement(1573..2913)
                     /locus_tag="VFB80_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN33820.1"
                     /translation="MIITRRALPRRTLLKGIGAALSLPLLDAMIPALTAEAKTPAEPT
                     RLKRIGYIYMPMGCDMSRWMPPGEERLDELSPTLSPLAPVKEHVTAITNMELQNAYPG
                     THATSNAAFLSAARAKWTESTDYYLGTTADQVAAQRIGHQTQLPSLEMSMDLLTTVGN
                     CDNGFACVYQNNLSWSSPTTPLPAEAHPRIVFERLFGDGGTPAERRAALKQRASLLDS
                     VTEEIARLQNQLGSADRAKVAQYLDSVREVERRIQKAETGAKDNPLPDLDRPVGVPAA
                     YADHARLMFDLQVLALQGDVTRVITFQLARETSNRTYPEIGVSDAHHPLTHHGNDPEK
                     IAKVALINRFHVSLFADFLQKLKATPEAGGTLLEHSLYLYGSGMGNPNVHDHTNLPIL
                     VAGGAAGGMRGGRHIKFDRPTPLANLHLTLLNKVGVRLDAFADSKGQIEGLFEI"
     gene            complement(2910..5267)
                     /locus_tag="VFB80_08375"
     CDS             complement(2910..5267)
                     /locus_tag="VFB80_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN33821.1"
                     /translation="MASDASRVDRRTLARWSLATMLVALPVAGVCRAEPPSRKAFVKE
                     YCADCHNSEDKTGGLALDVISSEEAQRRPEIWEKVVRKLRARQMPPAEMPRPDEAAYE
                     AMLAGLTSELDQAAKEHPRPGRTETFRRLTRTEYQNAVRDLLAVDVDAAALLPADESS
                     HGFDNITVADLSPTLLDRYISAAQKISQLAVGNPRRSPGGETFRPRADLTQEQHVAGL
                     PLGTRGGLLVPYTFPQEGEYEIQVRLTRDRNEEVEGLHEPHELVLLIGSEQAATFTVT
                     PPKDKDYASVDAHLKYRLHVAAGPHKVGVAFLKQPFSLLETRRQPYEARFNTHRHPRL
                     TPAVYQVSITGPFAAASGGDSPSRRKIFICHPDSTNEEEACGRRILATLARRAYRRPV
                     TDADLEKPLALFRQARAESGFDAGIELALSGILVNPNFLFRIERDPPGVAAGAAYKLS
                     DVELASRLSFFLWSSIPDDELLELAEAGKLSQQGVLKQQVRRMLADERSRSLATNFAS
                     QWLHLRNLDSITPDLRSFPDFDDNLRQAFRQETELLFDSVVRSDRSILELLRTDRTFL
                     NERLARHYGIPHVYGSRFREVTLPPQSERGGLLRQGSILTVTSYATRTSPVIRGKWIL
                     ENLLGTPPPPPPANVPALKDNTVAANLSVRARLAEHRANQACASCHQLMDPVGFALEN
                     FDAVGRWRSAEDFQPIDAAGGLPDGSKFTGVSGLEKALLDRPDLFAGTLAEKLLTFAL
                     GRGVEYHDGPAIRQIVREAREADYRFSALAEAIAASVPFQMRAAE"
BASE COUNT          884 a         1841 c         1904 g          988 t
ORIGIN      
        1 cgcatcaccc ccgcgggcat cgagaagctc aaaacgcact tcccgaagct gagcctgggc
       61 tactccgagc gcaagtagca gagctcaata gtccttgcgg ccgctcttcg gcgggtcatt
      121 cgtcggcgcg actccggcgg cgagcatcac cttgtgcatg gcggcgatcg tctctgccgc
      181 gggcttgaaa ttgccgaagc ggtagcccag ggcgatatgc agtggcgtcc agccgtgctt
      241 gttcgtcttg ttccaggtgt cgatcttcgc gcccttgtcg gcgagcagct gcaccatcct
      301 cggcatgctc ttgtaggccg cgccgtgcat ggcggattcg ccattgcgat cgacggcgtt
      361 gatgtcgctg cccagctcca agagcagcgc gaccgtttcg agcgcctcgg cctcggtgcc
      421 ggcctcttcg ccagggaagc gcgtgcccac gccggcagca gccaggagcg gggtgcagcc
      481 gtcggcgttg gcgatgagcg gatcggcgcc caattcgacc agtaacttga tcagcggcac
      541 gtcgtcgcga aaggccgcga gcaagagcgg cgtcgcgccg gatttgccca actggccgcc
      601 gcccgactcg ccccgcttga gtcgcaggtt cacgtcggcg ccgagcttga ccagctcgcg
      661 cacgaaatcg gggctgctca cgctgccaga gccgcggggg gcgggatcgc cgtcatcgcc
      721 ttcgccgcgg ttgggcttgc ggactgcaac cagcgcgtgc agcggggcaa agcccgagcg
      781 ctgatcgttg gggtccgcgc cggctttgac cagcgacagg gccagctcga agtggccgtt
      841 ctcgacggcc accagcagcg gactgatgcc gccgttgccg ccgccgccaa gtcggcgcga
      901 ggccgcggcc tcggtaacat ccgcgccggc agtcagcagc aactggacta catcggcatg
      961 gcccgcgcgg accgcgaaca agagcggcgt aaagccagag gccagcggct ggcggaactc
     1021 ggccttggca tcgagcagcg cttgcgcgac ttcgacatgc ccctcggccg ccgcccacat
     1081 gatggcggtc tgacccttgc gctccttggc gttcacatcg gccccggcag cgagcagggc
     1141 cttcaccggc gcgagccggc cggtgcgggc ggcggtcatc agtgccgtct cgccgccgcg
     1201 gagcttcgtg ttaggatccg caccactctt gagcagcagc tcgaccagct cgccgttgcc
     1261 gttctcgcag gccaatggca gcggcgcgac gccgtagcgg ttctccagcc ggacatccgc
     1321 gccggcggcc agcaaccggc gggccagttc cacgtcgtcg tgatgagcgg cccagtgcag
     1381 tgcagtcatg ccgtcgggct gggcctgatt gacggccgcc ttgtcactca gcagcgactc
     1441 gaccctcgcc cggtcgcttc gctcggccgc atcggccaag ggagaatcgt cacgggcggc
     1501 agcggccgtg gacaagcacg cggccgcgag catccccaga agcagccgac gatggcacat
     1561 gacgccgctc cgctagattt cgaacagccc ctcgatctgg cctttgctgt cggcgaacgc
     1621 atcgagccgg acgccaacct tgttgaggag cgtcaggtgc aggttggcca gcggcgtcgg
     1681 gcgatcgaac ttgatgtgcc ggccgccgcg cattccgccc gccgctccgc cggcaaccag
     1741 gatcggcagg ttcgtgtggt cgtgaacatt gggattgccc atgccgctgc cgtacaggta
     1801 gagcgagtgc tccagcaacg tgccgccggc ttcgggcgtc gccttgagct tctgcaagaa
     1861 gtcggcgaac agcgacacgt gaaagcgatt gatgagggcg accttggcga ttttctcagg
     1921 atcgttgccg tggtgcgtga gcgggtgatg ggcgtcgctc acgccgatct cgggataggt
     1981 gcggttgctc gtctcgcggg ccagctggaa cgtgatcacg cgggtcacgt cgccctgcag
     2041 cgcgagcacc tgaagatcga acatcagccg ggcgtgatcg gcgtaggcgg ccggcacgcc
     2101 gacagggcgg tcgaggtccg gcagcggatt gtccttcgcg ccggtttcgg ccttttgaat
     2161 gcggcgctcg acttcgcgga ccgagtcgag atactgagcg accttggccc ggtcggccga
     2221 gccgagctga ttttggagcc gggcgatctc ctcggtgacc gaatcgagca ggctcgcccg
     2281 ctgcttgagg gccgcacgcc gctcggccgg agtgccgccg tcgccgaaca gccgctcgaa
     2341 gacgatccgc ggatgggctt cggccggcag cggcgtcgtg ggcgacgacc aggagaggtt
     2401 gttctgatag acgcaggcaa acccgttgtc gcagttgccg accgtggtca gcaggtccat
     2461 cgacatttcc agcgacggca gttgcgtctg gtggccgatc cgctgggcgg caacctggtc
     2521 ggcggtggtg ccgaggtaat agtcggtgct ctcggtccac ttggcgcggg ccgcgctcag
     2581 gaacgcggcg ttcgacgtgg cgtgcgtccc gggataggca ttctgcaact ccatgttcgt
     2641 gatcgccgtc acgtgctcct tcaccggggc cagcgggcta agcgtgggcg agagctcgtc
     2701 cagccgctct tcgcccggcg gcatccagcg gctcatgtcg cagcccatcg gcatatagat
     2761 gtagccgatc cgcttcagcc gggttggctc cgcgggcgtt ttggcctcgg ccgtgagggc
     2821 cggaatcatc gcgtcgagca gcggcaggga cagcgccgcg ccgatcccct tcagcaacgt
     2881 ccgccgcggc aaggcacgtc tggtgatgat cattcggcag ccctcatttg aaacggcacg
     2941 ctggcggcga tcgcctcggc cagggccgag aagcgatagt cggcctcacg cgcctcgcga
     3001 acgatctggc gaatcgccgg accatcatga tactccacgc cgcgtcccag cgcgaacgtc
     3061 agcagtttct cggccagcgt cccggcgaaa agatccgggc gatcgagcag ggctttttcc
     3121 aggccggaca ctcctgtgaa cttgctgccg tcgggcaggc cgccggccgc gtcgatcggc
     3181 tgaaagtcct cggcactccg ccagcggccg accgcgtcga agttctccag cgcgaagccg
     3241 acggggtcca tgagctggtg gcagctggcg caggcctggt tggcgcggtg ctcggccagg
     3301 cgggcccgca ccgagagatt ggccgcgacc gtgttgtcct tgagcgcggg cacgttggcg
     3361 ggcggcggag gaggaggcgt gcccagcagg ttctcgagaa tccacttgcc gcggatcacg
     3421 gggctggtgc gggtggcata ggaagtcacc gtcaggatgc tcccctgccg caacaggccg
     3481 ccgcgctccg actgcggcgg gagcgttacc tcgcgaaacc ggctgccata gacatgcgga
     3541 attccgtaat ggcgcgccag ccgctcgttg agaaacgtgc gatcggtccg cagcagctcc
     3601 aggatgctgc ggtcgctcct gaccacgctg tcaaacagca gctccgtttc ctggcgaaag
     3661 gcttgccgca ggttgtcgtc gaaatcggga aacgaccgca agtcgggcgt gatcgaatcg
     3721 aggttgcgga ggtgcagcca ctggctggca aagttcgtgg ccagcgaccg cgagcgctcg
     3781 tcggccagca tccggcggac ttgttgcttg agcacgccct gctggctcag cttgccggct
     3841 tccgccagct ccagcagctc gtcgtcggga atgctgctcc acagaaagaa tgacagccgc
     3901 gacgccaact ccacgtcgct tagcttgtag gctgcgccgg cggcgacgcc tggcggatcg
     3961 cgctcgatgc ggaacaagaa gttcggattg acgagaatcc cgctaagtgc cagctcgatc
     4021 cccgcgtcga agccgctctc ggcccgcgcc tggcgaaaga gagccagcgg cttttccagg
     4081 tcggcatccg tcaccggccg ccggtaggcc cgccgggcca atgtcgccag gattcgccgc
     4141 ccgcatgctt cctcttcgtt cgtgctgtcg ggatggcaaa tgaaaatctt gcggcggctc
     4201 ggtgaatcgc ccccggaggc cgctgcgaat ggcccggtaa tcgacacctg gtacaccgcc
     4261 ggcgtcagcc gcgggtgccg gtgtgtgttg aagcgggcct cgtacggctg ccgccgcgtc
     4321 tccagcagcg aaaacggctg cttgaggaac gccacgccga ccttgtgcgg acctgccgcg
     4381 acgtgcaggc ggtatttgag gtgcgcatcg accgacgcat agtccttgtc cttcggcggc
     4441 gtgacggtaa acgtggccgc ctgctcgctg cctatgagca acaccagctc gtgcggctcg
     4501 tgcaggccct cgacctcttc gttgcggtcg cgagtcaggc ggacttgaat ttcgtattcg
     4561 ccctcctgcg ggaaggtgta gggcacgagc agaccgccgc gcgtgcccag cggcaggccc
     4621 gcgacgtgct gctcctgcgt gaggtcggcc cgcgggcgga aggtctcgcc gccggggctg
     4681 cgccgcgggt tgcccaccgc caactggctg attttctggg cggccgagat atagcggtcg
     4741 agcagcgtcg gcgagagatc cgcgacggta atgttgtcga agccatggct cgattcgtcg
     4801 gcgggcaaca gcgcggcggc gtccacgtcg accgccagca ggtcgcggac ggcgttttga
     4861 tattcggtgc gcgtcaggcg tcggaacgtt tccgtacgcc cgggacgggg atgctccttc
     4921 gccgcctggt cgagctcgga cgtgagccca gccagcatcg cctcataggc cgcctcgtcg
     4981 ggccgcggca tctcggcggg aggcatctgg cgggcgcgga gcttgcggac gaccttttcc
     5041 cagatttcgg gccgccgctg ggcttcctcg ctgctgataa cgtccagcgc gaggccgccc
     5101 gttttgtctt cgctgttgtg gcagtcggcg cagtattcct taacgaatgc cttgcgcgaa
     5161 ggcggctcgg cgcggcagac gccggctact gggagcgcga ccagcattgt ggcaagcgac
     5221 caccgcgcca gcgtccgccg atcgactctt gaagcgtctg acgccattcc ggccaactcc
     5281 gattcggaag gggagtgtag ccgcggatgg acgcggataa cacggatcgg aaatgatttg
     5341 tctgatccgt gtcatccgcg ttcatccgcg gccattctta ccctcctacg acccagttcg
     5401 agtagcggca attcccccgg atttccgatt ctgaatgcag tggtgacatg cccttgcggc
     5461 ttcggcccca agttcgaaat tgcattttgg catcttcgtc gccaaaacgc cacaagttcg
     5521 gccggcaaat aacgtcaagc cagaattccg ggcgggtttg tggacttaac ggatttttca
     5581 gtccttctgc aacacacgct cacgattttg tcgctcg
//
LOCUS       DASRJB010000311         5587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4037926, whole genome shotgun sequence.
ACCESSION   DASRJB010000311 DASRJB010000000
VERSION     DASRJB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037926"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFB80_08380"
     CDS             complement(<1..556)
                     /locus_tag="VFB80_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN33822.1"
                     /translation="MAADAPLADAAERQDRASVRRLLDERADVNQPQADGMTALHWAT
                     YHDDLELAARLIRAGADVKAANAYGVLPLPLACTNGNAELVELLLEAGADPNTTQRGG
                     ETVLMTAARTGKLAPVKALLSRGADVNAKERKGQTAFMWAAAEGHADVVQALIDAGAE
                     FRTPLASGFTPLMFAAREGRIAVAQ"
     gene            complement(628..1974)
                     /locus_tag="VFB80_08385"
     CDS             complement(628..1974)
                     /locus_tag="VFB80_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN33823.1"
                     /translation="MIVTKKALPRRTFLRGLGVALSLPLLDAMIPALTAQANTPADPA
                     GLRRLGFVFMPMGCDQSRWTPKGDRLDELSPTLEPLAPIKQHVTAITNLELRNAYPGT
                     HATSNAAFLSAVTAKRTESTDYYLGTTVDQIAAKQIGQETQLPSLEMSMDLLSTVGQC
                     DNGYACVYQNNLSWSSPTTPLPAEAHPRIVFERLFGEGGTPAQRRAALKKRASLLDAV
                     TDEITRLKGSLGPADRAKVSQYLDTVREVERRIQKAEASTKDNPLPDLDRPVGVPASY
                     ADHARLMFDLQLLALQGDVTRVITFQLARETSNRTYPEIGVPDAHHPLTHHGNDPEKI
                     AKVAKINRFHVSLFAEFLEKLAATREGDGSLLDHVLYLYGSGMGNPNVHDHTNLPILV
                     AGGAAGRMRGGRHIQYESATPLANLHLTLLDKVGVRLESFADSKGQIEELFEPLAM"
     gene            complement(1971..4331)
                     /locus_tag="VFB80_08390"
     CDS             complement(1971..4331)
                     /locus_tag="VFB80_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN33824.1"
                     /translation="MSRGIKWTCLGTPLLLAGGFSGICGAQQPAESPPGPARQRALVS
                     QYCADCHGGQEKTAGLDLDTISKTDVSNHADVWEKAVRKLRVRQMPPADAPRPDERTY
                     RDVIAFLETSLDKAAAERPRPGRTATLRRLTRTEYQNAIRDLLALEIDAATLLPPDES
                     SRSFDNVGVSDLSPALLDRYVSAAQKISALVVGGSSRSPGGDTIRIRPDITQEEHVPG
                     LPLGTRGGALIPYYFPQDGEYEIQVRLARDRNEEIEGLKEPHELVVLLDRKQAASFTI
                     EPPKDANHQHVDAHLKVRLKVPAGPHQLGVTFLKQPSSLLETRRQPYQAHFNMHRHPR
                     ITPAVYQVSIVGPYEAEGAGDTPSQRRVFVNRPTKPDDEESCARQILSALIRRAYRRP
                     VTDAEVEKPLALYREVRAEGGFDGAIEMAVSSVLVNPQFLFRIERDPEGIAAGVTYRV
                     SDLELASRLSFFLWSSIPDDELLNLAEAGELSQPVVLEKQVRRMLADVRSQSLVTNFA
                     SQWLHLRNLDATTPDLRLFPDFDNNLRQSLRQETELLVASVLREDRSVLDLLKADYTF
                     LNERLAKHYEIPHIYSSRFRRVSLDKQSERGGLLRQGSILTVTSYPTRTSPVIRGKWV
                     LENLLGAPPPPPLPDVPALKDNTVSSNLSVRQRLAEHRSNAACASCHNLMDPIGFSLE
                     NYDAVGRWRTLEEAKPIDASGGLPDGSQFTGASGLEQALLKRPELFVGTMTEKLLTFA
                     LGREIEPYDGPAVRQILRDAKADNYRFSSLIVGIAKSTPFQMRTAP"
     gene            complement(4469..5398)
                     /locus_tag="VFB80_08395"
     CDS             complement(4469..5398)
                     /locus_tag="VFB80_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN33825.1"
                     /translation="MDQLAARRRVLTLGAAAAVAAMAPSAAAADEPRPPKPPRVDAHL
                     HCFAGQKDARFPYHARAPYEPEQAATPEHLLDCMQQAEVDHAVVVHPEPYQDDHRYLE
                     HCLRVGKGKLKGTLLLFADREGSLDKMPELCRKLDVISVRVHAYAPDRLPPFGKPELK
                     RLWSMAGEHGLAVQLHFEPRYAPGFEPLIREFKSTPVIIDHLGRPFQGTPQEHEVVIR
                     WARFDNTIMKLSAIPEKTTYPHRDIAPVIRQLTDAWGAERMIYGGGFSAAATGDSYRQ
                     AFEKARSQLTHLSAADQAKIFGGTAARVFSLAT"
BASE COUNT          929 a         1835 c         1785 g         1038 t
ORIGIN      
        1 cctgcgccac ggcaatccgc ccttctcgcg cggcaaacat tagcggcgta aaaccggacg
       61 ccagcggcgt gcggaattcg gcgccggcgt cgatcagcgc ctggacgacg tcggcatgtc
      121 cctcggccgc agcccacatg aatgccgtct gtcctttgcg ctctttggca ttgacatccg
      181 cgccgcggga cagcagcgcc ttcactggag ccagcttgcc tgtccgcgcg gcggtcatga
      241 gcacggtctc accgccgcgc tgcgttgtat tgggatctgc accggcttcg agaagcagct
      301 caaccagctc cgcattgccg ttggtgcagg ccagtggcag cggcaacacg ccatacgcgt
      361 tggcggcttt cacgtccgca ccggcgcgga tcaagcgcgc cgccagttcc agatcgtcgt
      421 gatacgtggc ccagtgcagg gccgtcatcc cgtcggcctg cggctgattc acatcggcgc
      481 gctcgtccag cagccggcgc acgcttgctc gatcctgcct ctccgccgcg tccgccagcg
      541 gcgcatcggc ggccacagtg gccgagctgg cggcaagcac cgctgaagcc aacgcacagg
      601 caatcgattt gtacgaattc attgccatca catcgccagc ggctcgaaca gctcctcgat
      661 ctgtcccttg ctgtcggcaa aggattcgag tcgcacgccg accttgtcca ggagcgtcag
      721 gtgcaaattg gccagcggcg tcgcactttc atactggatg tgccgcccgc cgcgcatccg
      781 cccggccgca ccacccgcga cgaggatcgg caggttcgtg tggtcgtgta cgttggggtt
      841 ccccatgccg ctgccataga gataaaggac gtgatcgagc agcgatccgt ccccttcgcg
      901 cgtcgccgcc agcttctcga ggaactcggc gaacagcgac acgtggaagc ggttgatctt
      961 cgccaccttg gcgatcttct ccggatcgtt gccgtggtgc gtgagcggat gatgcgcatc
     1021 gggaacgccg atctcgggat acgtgcggtt gctcgtctcg cgggcaagct ggaacgtgat
     1081 cacgcgggtc acgtcccctt gaagcgccag gagctgcaag tcgaacatca gccgcgcatg
     1141 gtcggcatac gaggccggca cgccgaccgg gcgatcgagg tccggcagcg gattgtcctt
     1201 ggtgctggcc tcggccttct ggatgcggcg ctccacctcg cggaccgtgt cgagatactg
     1261 gctcactttg gcccgatcgg ccggtcccag gctccccttc agccgcgtga tctcgtcggt
     1321 gacggcatcg agcaggctgg cccgcttctt gagtgccgcc cgccgctgcg ctggagttcc
     1381 cccttcgcca aacagtcgct cgaagacgat ccgcggatgg gcttccgccg gcagcggcgt
     1441 cgtcgggctc gaccaggaga gattgttctg atagacgcag gcgtagccgt tgtcacactg
     1501 ccccaccgtg gacagcaggt ccatcgacat ctccagcgac ggcagttgcg tctcctggcc
     1561 gatctgcttt gctgcgatct gatcgacagt cgtacccaga tagtagtcag tgctctcggt
     1621 ccgcttggcg gtgaccgcgc tcaaaaaggc agcattcgaa gtcgcgtgcg tgccaggata
     1681 ggcgtttcgc agctccaagt tggtaatcgc cgtgacgtgc tgcttgatcg gcgctagcgg
     1741 ctcgagcgtc ggcgaaagtt cgtccagccg atcccccttc ggcgtccagc gactctggtc
     1801 gcagcccatg ggcatgaaga caaaccccag ccgccgcagg ccggccgggt cggccggcgt
     1861 gttggcctgg gccgtgagcg ccggaatcat cgcatcgagg agcggcagcg acagggccac
     1921 gcccaatccg cgcaaaaagg tccgccgcgg cagtgccttc ttcgtgacga tcatggggct
     1981 gtcctcatct gaaagggcgt gcttttggca atgccgacga tgagcgacga gaagcggtag
     2041 ttgtccgctt tggcgtcgcg caggatctgg cgaacggccg gaccgtcgta cggttcgatc
     2101 tcgcgcccca aggcaaacgt cagcagcttc tcggtcatcg tgcccacaaa gagctccggc
     2161 cgtttcaaaa gcgcttgctc caggccgctt gcgccggtga actggctgcc gtcgggcagg
     2221 ccgccggaag cgtcgatcgg cttggcttct tccaaggtcc gccagcgtcc gacggcgtcg
     2281 tagttttcca gcgagaagcc gatggggtcc atcaggttat ggcagctcgc gcaggcggca
     2341 ttgctgcgat gctctgccag ccgctgtcgg acggagagat tcgacgagac cgtgttgtcc
     2401 ttcagggccg gcacatcggg gagcgggggc ggagggggcg cgccgaggag attctccagg
     2461 acccatttgc cgcggatcac gggcgaagtg cgcgtgggat acgaggtgac ggtgaggatg
     2521 ctcccctgtc gtaacaggcc gccacgctcg ctctgtttgt cgagcgaaac gcggcggaag
     2581 cggctgctgt agatgtgcgg aatctcgtag tgcttcgcca gccgctcgtt aaggaaggtg
     2641 taatccgcct tcaagagatc gagcacgctg cggtcctcgc ggaggacgct ggccaccaaa
     2701 agttcggtct cttgccgcag cgactgccgc aggttgttgt cgaaatcggg aaacagacgc
     2761 aggtcgggag ttgtcgcatc cagattgcgg agatggagcc actggctggc gaaattcgtc
     2821 accaggctct gcgagcgcac atcggcgagc atccgccgca cttgcttttc gagcacgacg
     2881 ggctggctga gttcaccggc ctcggcgaga ttcaaaagtt cgtcgtccgg aatgctgctc
     2941 cagaggaaaa acgacagccg cgaggccagt tccaggtcgc tgacgcggta ggtgacgccg
     3001 gcggcaatcc cctctggatc gcgctcgatg cgaaacaaga actgcggatt caccaggacc
     3061 gagctgacag ccatctcgat cgcaccgtca aagccaccct cggcccgcac ttcgcggtac
     3121 agggccagcg gcttttccac ttcagcgtcg gtgacgggcc gccgataggc gcggcgaatt
     3181 agagcgctga gaatttgcct ggcgcacgat tcctcgtcgt ccggcttcgt cggccggttg
     3241 acaaaaactc tccgctggct gggggtgtcc cccgctccct cggcttcgta tgggccgacg
     3301 atcgagacct gatacacggc cggcgtgatc cgcggatgcc ggtgcatgtt gaagtgcgcc
     3361 tggtacggct gccgcctggt ttccagcagc gacgaaggtt gcttcagaaa cgtcacgccg
     3421 agctggtgcg ggccggccgg cactttaagc cgcaccttga ggtgcgcatc gacatgctga
     3481 tggttggcgt ctttgggcgg ctcgatcgta aatgacgccg cttgcttgcg atcgaggagc
     3541 accaccagct cgtgtggctc cttcagcccc tctatctcct cgttgcggtc ccgcgccagc
     3601 cgcacctgaa tctcgtactc gccgtcctgc ggaaagtagt atggaatcag cgcgcccccg
     3661 cgcgtcccca gcggaaggcc tggaacgtgc tcctcctgcg taatgtccgg ccgaatccga
     3721 atcgtgtcgc cgccggggga tcggctcgag ccgccgacca ccagcgcact gatcttctgc
     3781 gccgcggaga cgtagcggtc cagcagcgcc ggcgagaggt cgctcacgcc gacgttgtca
     3841 aagctgcggc tcgactcgtc agggggcaag agtgtcgccg catcgatctc gagggccagc
     3901 agatcgcgga tcgcgttttg atattccgtg cgagtcagcc gccgcagcgt ggcggtgcgc
     3961 cccggccgtg gtcgctcggc ggctgccttg tcgagcgagg tttccagaaa ggcaatcacg
     4021 tcccggtacg tgcgctcgtc gggccgcggc gcatcggccg gcggcatctg ccgcacgcgc
     4081 agcttgcgga ccgctttttc ccaaacatcg gcgtgattgc tgacatccgt tttgctgatc
     4141 gtgtccagat cgagcccggc cgtcttctcc tggccgccgt ggcaatcagc gcagtactgg
     4201 ctcaccagcg cacgctggcg ggccggccct ggcggacttt ctgccggctg ctgcgccccg
     4261 caaatgccgc tgaaaccgcc cgccagcagc agcggcgttc ccaggcaagt ccacttgatc
     4321 ccgcgactca tgagatcagg ctccggcggc aatttaccca tgaatcagtg tgatcggacc
     4381 ttggggagca aaccaatcgc cagcctgatt ttacgtgatt gctgcggcgc ggtgcaaatg
     4441 ctcatcgcag tcgccggccg ccgcgacact acgtcgccag ggaaaacact cgggccgcag
     4501 tgccgccgaa aatcttggcc tgatcggcgg ccgagaggtg ggtcagttgc gagcgtgcct
     4561 tctcaaatgc ctggcggtag ctgtcaccgg tcgcggccgc actaaaaccg ccaccgtaga
     4621 tcatccgctc ggcgccccag gcgtccgtga gctggcggat gacgggagcg atatcccgat
     4681 ggggatacgt cgttttttct ggaatcgccg agagcttcat gatcgtgttg tcgaaccggg
     4741 cccagcgaat caccacttcg tgctcctgcg gcgttccctg aaacggccgc cccaggtggt
     4801 cgatgatcac gggcgtgctc ttgaattcgc gaatcagcgg ctcaaagccc ggagcgtacc
     4861 gcggctcaaa gtgcagttgc accgccaggc cgtgctcgcc cgccatgctc cacagtcgct
     4921 ttagctccgg cttgccaaac ggcggcagcc gatcgggcgc ataggcgtgc acccgcacgc
     4981 ttattacatc cagcttgcga cacagctcgg gcatcttgtc gagcgatccc tcgcggtcgg
     5041 caaatagcag cagcgtccct ttcagcttcc ctttgccaac tcgcaggcag tgctcgagat
     5101 agcgatggtc atcctggtac ggttcgggat gcaccaccac cgcatggtcc acctccgcct
     5161 gctgcatgca gtccagcaaa tgctccggcg tggcggcctg ctcaggctcg tacggtgcgc
     5221 gggcgtgata aggaaaacgc gcgtccttct ggccggcaaa gcagtgcagg tgagcgtcga
     5281 cgcgcggcgg cttaggcggc cgtggttcgt ccgcagcggc tgccgatggc gccattgccg
     5341 ccaccgccgc ggcggcgccc agcgtcagaa cccggcgacg cgctgcgagc tggtccattt
     5401 tgcgattccc tcttagccgt agcgagccgc aaactacttt ccatcgccac cggcggcggc
     5461 aaagtcgccg gccaccacaa ccaccagccg ctcgcgatcg atgtgcttcc ttagcgcggc
     5521 atgcacttcg tccggcgtga gcgaagcaac cttggcctcc agtgccgctt cgtactcgag
     5581 cgtctgc
//
LOCUS       DASRJB010000312         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3471531, whole genome shotgun sequence.
ACCESSION   DASRJB010000312 DASRJB010000000
VERSION     DASRJB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471531"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFB80_08400"
     CDS             complement(<1..518)
                     /locus_tag="VFB80_08400"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZN33826.1"
                     /translation="MPSPLSTPEKKKTSLRRVAILFAGGPAPAANAVISTAAASFLRN
                     DCEVLGIMYGYSQLAEFSPEKPLVEGGHYVRLTHHELKRSRNTQGIMIGTSRTNPGRN
                     VSQPAHLDDPKLAAPLRRVYEALVSLEVDALISIGGDDTLKTANKLKLYQDRLPAAAR
                     RIPVVHLPKTID"
     gene            complement(764..1654)
                     /locus_tag="VFB80_08405"
     CDS             complement(764..1654)
                     /locus_tag="VFB80_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33827.1"
                     /translation="MSSPENPPAQNRPRRWSSGRLMWLFLLIMVGPCTVMQVPLEIGR
                     WKLAAAIQAREAGDKERAYAELNAAMNWVADRPALIAQQAKWHLADGQVEEGLAVADK
                     LLESAPDSSEALRAHSRLLQMAGRFEDAIADLKKVDQNSQRTGVPSRAEALNELAYAQ
                     ALAEVELDDALKNVDESLELLTKEEKRTGLELAIRDTRGYILFLKGRYAEALVDMNFA
                     VAPLNPSAVAKKQLDFHRLPPKVQRPLLHKFRQEFQSIAVMYYHRGLVFQALGRQPEA
                     AADLQRARSLIGREPDETLF"
     gene            2139..3143
                     /locus_tag="VFB80_08410"
     CDS             2139..3143
                     /locus_tag="VFB80_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33828.1"
                     /translation="MTRTLVVWSIVGCLGFSAYAQEAKPLADAAAGEIAELIDQLEGA
                     EFAERQEATRKLSEVGRAVIPQLEKVAAGSSREASVRAFDVIGGHFQRGDNETKASAK
                     AVLDRLAASTNPAIAQRAQNILNPPPVPTAAEVAALAGVPRLANLQVQMAAIPAAARS
                     VTVSRDANGGIRIQLRENAKTTKIESLPGGKITAEITEKQNGQEVTKKIEAADVGELK
                     KKDAEIARIFEQYNQPARIGFAPLRPAAPLPVGGFGRPAPIQPADTLKRRIESLDGQI
                     KRLKEGAPQTIATKGMLDELQTMRARYQRQLEEIEKPAQPAPGNAPQPAEAEKGVGGQ
                     "
     gene            3149..>3759
                     /locus_tag="VFB80_08415"
     CDS             3149..>3759
                     /locus_tag="VFB80_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZN33829.1"
                     /translation="MSIFAARRDNLRRLLAEKQLPALLVTDERNVTYLTGFTGDSSYL
                     LVARERELLITDRRYETQLGEECPGLDLAIRGPGTKLPDFTAETVGKLELPCLGIEAD
                     TLTVGAYEKFRTVMKTTQWASTSGLVEKLREVKDAGEIEEIRRAVRLAERAFAVTRAS
                     LRLGRSEKEVADELEYQIRLFGGACGAFPSIIGVGPRAALPHGR"
BASE COUNT          712 a         1209 c         1199 g          639 t
ORIGIN      
        1 ttgtcgatcg tcttgggcag atgcaccacg ggaatccgcc gggccgcggc gggcaggcga
       61 tcctgataga gcttgagctt gttggccgtc ttcaaggtat cgtcaccgcc gatactgatc
      121 agggcatcga cctccagcga caccagcgct tcatagaccc gccgcagcgg cgcggcgagc
      181 ttggggtcgt cgaggtgtgc cggctgcgag acgttcctgc cgggattcgt gcgggacgtg
      241 ccgatcatga tcccttgcgt gttgcgggac cgtttcaact cgtggtgcgt caggcggaca
      301 tagtgcccgc cttcgaccag cggcttctcg ggcgagaact ccgccagttg cgaatagccg
      361 tacataatgc cgagcacttc gcaatcgttg cgaaggaacg acgcggcggc cgtgctgatg
      421 accgcgttgg ccgccggggc ggggccgccc gcaaagagaa tcgccacgcg ccgcaaggag
      481 gtcttcttct tttccggcgt cgagagtgga ctgggcatgg tcggactcac agacaagcaa
      541 tggaacggcg cgtcaaacac gaattgtagg acacgctgtc gcggcaggcc aaggcaaacg
      601 gcctccgcag caatggcaaa tcgactacag ccggcatctg tccctaggac gcgttttgcg
      661 ccgccgcacc cgccgttatt cgcttaaccc ggagcccgag gcgacggcgt ttccccatag
      721 ccgccgtcgc ctccggctcc gggttaaacg aaccgatcga caatcagaac agtgtttcgt
      781 cgggctcgcg gccgatcagc gaccgcgcgc gctgaagatc ggcagcggcc tccggctgac
      841 ggccgagcgc ttgaaacacc agcccgcgat ggtagtacat caccgcgatg ctttgaaatt
      901 cctgccggaa tttatgcagc aacggccgct gaaccttcgg cggcaggcga tggaagtcca
      961 gctgcttttt ggcgacggcg ctcgggttca gaggggccac ggcaaaattc atatcgacga
     1021 gcgcttcggc atagcggcct ttcaggaaca ggatgtaacc gcgcgtgtcg cgaatcgcca
     1081 gctcgagtcc cgtgcgtttc tcttccttgg taagcagctc cagtgactca tcgacattct
     1141 tgagcgcatc gtccagctca acctcggcta gcgcctgggc gtaagcgagc tcattgagcg
     1201 cttcggcgcg cgacggaact ccggtgcgct ggctgttctg atcgaccttc tttaagtccg
     1261 cgatcgcgtc ctcaaagcgg ccggccatct gcagcaagcg gctatgcgcc cgcagcgcct
     1321 cgctggaatc gggcgcggac tctagcagct tgtcggcgac ggcgagtcct tcctcgacct
     1381 gaccgtcggc cagatgccac ttggcctgct gcgcgatgag cgccgggcgg tcagccaccc
     1441 agttcatcgc tgcgttcaac tcggcatacg cccgctcttt gtcgccagcc tcgcgagcct
     1501 ggatcgcagc cgccagcttc cagcggccga tttccagcgg cacctgcatg accgtgcagg
     1561 gcccgaccat gatcagcagg aacagccaca tcaaacggcc ggaactccag cggcgagggc
     1621 ggttttgggc cggcgggttt tcgggagaac tcatttcgcg aggcagatgg tacggataat
     1681 ctagtctctg gattttattc aggcccgccg ccggggaaac ccgacaattc ttccttcatc
     1741 ctctgcggct gccgggcttc gccgacgtcc ggcgctaatg tcggccgccc cgaaaggggt
     1801 ggcttgcgcg cgcctttcat cgccagtttt cgtcgctatc tctgttggct caagggcttg
     1861 cgtcgaatgc tcgccaaccg ggttaaatac tacttattag gcttagagaa cgcctctgta
     1921 cgacaagacc ccagagcggc ccaggccgat gaatcgccaa aatggcagtc ctgccgcgca
     1981 atgggaacta tactgcaaat accgtaccaa tgttcatgaa tcgcgaagta ccgctcctca
     2041 cggccccccg ctgcacgggg gacagccttg aggtatcatt accaatcttg atagccgcag
     2101 gtgcggtcgc gccatcatta ccggtctgga ggtcgatcat gactcgaaca ctggtagtct
     2161 ggtccatcgt cggctgcctg ggtttctcgg cctacgccca agaggcaaag ccgctcgccg
     2221 atgccgcggc cggcgaaatc gccgagctga tcgatcagtt ggaaggagcc gaattcgccg
     2281 agcggcagga agccaccaga aagttgagcg aagtaggccg ggccgtgatt ccgcaactgg
     2341 aaaaggtcgc ggccggcagc agccgcgagg cgtcggtgcg ggccttcgac gtgattggcg
     2401 ggcattttca gcgcggcgac aacgagacca aagcctccgc caaggcggtc ctcgatcggc
     2461 tggcggccag caccaatccg gccatcgccc agcgggccca gaacatcttg aatcctccgc
     2521 ccgtgccgac ggcggccgag gttgcggcgc tggcgggagt gccgcggctg gcgaatctgc
     2581 aagtgcagat ggctgcgatc cccgcggccg ctcgcagcgt gacggtcagc cgcgacgcca
     2641 acggcggcat ccggatccag ctccgcgaga acgcgaagac gacgaagatc gagtcgcttc
     2701 cggggggcaa gatcacggcc gagatcaccg agaagcagaa cggccaggaa gtgacgaaga
     2761 agatcgaggc cgccgatgtg ggcgaactga aaaagaagga cgccgaaatc gcccgcattt
     2821 tcgagcagta caatcaaccc gcgcggattg gcttcgctcc cctgcgccct gccgcgccgc
     2881 tgcctgtggg cggcttcggt cggccggctc cgattcagcc ggcagatacg ctcaagcgac
     2941 ggatcgagag cctggacggt cagatcaagc gcctgaagga gggtgcgccc caaaccatcg
     3001 ccaccaaagg catgctcgac gaattgcaga cgatgcgagc gcggtaccag cggcaactgg
     3061 aggagatcga aaagcccgcc cagcccgccc ctggcaatgc gccgcagccc gccgaggccg
     3121 aaaaaggcgt gggagggcag taggcgatat gtcgattttc gccgcccgcc gcgacaatct
     3181 gcggcgtctc cttgccgaga agcagcttcc ggcgctcctg gtcaccgacg aacgcaacgt
     3241 cacgtatctc acgggcttca ccggcgactc gagctacctg ctcgtcgccc gcgagcgcga
     3301 gctgctgatc accgaccgcc gctacgaaac gcaactgggc gaggaatgtc ccggcctgga
     3361 cctcgcaatc cgcggtccgg gcaccaagct gcccgatttc accgcagaaa cggtcggcaa
     3421 gctcgagctg ccctgtctgg gcatcgaggc cgacacgctc accgtcgggg cttacgagaa
     3481 gttccgcacc gtgatgaaga cgacgcaatg ggccagcacg tcgggcctgg tggaaaaact
     3541 tcgcgaggta aaagacgcgg gcgagatcga ggaaattcgc cgcgccgtgc gcctggcgga
     3601 gcgggccttc gccgtcacgc gggcgtcgct ccgcctgggc cgctccgaaa aggaagtcgc
     3661 cgacgagctc gaataccaga tcaggctgtt tggcggcgcc tgcggggcgt tcccttcgat
     3721 catcggcgtc gggccgcggg cggcgctgcc gcacgggcg
//
LOCUS       DASRJB010000313         9602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2055947, whole genome shotgun sequence.
ACCESSION   DASRJB010000313 DASRJB010000000
VERSION     DASRJB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055947"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /gene="ilvC"
                     /locus_tag="VFB80_08420"
     CDS             complement(<1..734)
                     /gene="ilvC"
                     /locus_tag="VFB80_08420"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZN33830.1"
                     /translation="MPATIYYDNDADLSVLKGKTIAILGYGSQGHAQAQNLRDSGVSV
                     IVGQRPGSPNYELAVKHGFKPLAAEDATKQADIVNILLPDEVQGDVYRTSIRPNLKKG
                     AVLMASHGFNMHFGQVEPPPGVDAMLVAPKGPGHLVRSEFEKGGGVPCLIAIYPGSSE
                     STLKVGLAYAKGIGGTRGGVIQTTIAEETETDLFGEQCVLCGGVSELVKAGFDTLVEA
                     GYQPEMAYFECMHELKLIVDLFYQGGL"
     gene            complement(775..1320)
                     /gene="ilvN"
                     /locus_tag="VFB80_08425"
     CDS             complement(775..1320)
                     /gene="ilvN"
                     /locus_tag="VFB80_08425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZN33831.1"
                     /translation="MRHVLSAVVQNVPGVLAHISGMLASRGYNIDSLAVGETEDPNLS
                     RMTFVVVGDDRVLDQVRKQLEKIVTVVQVDDISSRDHVERDLMLLKVEAPPGQKRSEV
                     RELVEIFRAQIVDVGAEEVMIEISGRESKIEAFIQRMRPYGILELVRTGRIAMVRGKY
                     RQPKTEEEAEQQDAALKKFAG"
     gene            complement(1452..3002)
                     /locus_tag="VFB80_08430"
     CDS             complement(1452..3002)
                     /locus_tag="VFB80_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33832.1"
                     /translation="MTVTIRSWMALAVSGCVLLLAPSAQAQCGCGCPSFRIVNKVVYD
                     QVPVTTWRLEYETVLEDQQVTTLRPQWTDELRERRYTVAKPVTETATREERVLLRKPV
                     YETEYREESYDKVSYVTETQTRQERYQVQRPVVETQMREQQYTVRKAYQETVMQEQAY
                     TAYQPVTSMHTQYVDQGQYVTAYQPTAPVVRNRLWCVPSGYAADPATGQTTYYRGGLR
                     WVPTPGPSVLRPVTAYMPNIVAQQVATTSYQPVQMTQQVPVTVNRYYDELQTQQVPIT
                     VQRMETYEEVRDVPFTVQKPVTERVTYQVPVQKMRWEEEVQVRQIPYTVQRIEYEEVV
                     EQTPIKVCRYVNETRSVQVPRTVAKWVAQTSMRLQPRIETYRVPLTSYYGDAVYGPTT
                     TYYRSTPIPPAPPAAPPAALPPAALPPRTDVNKPPVNSGTPATDKKTNGTPPAGEPKP
                     AGTQPEEKKTEGTPAGDASKPANAEKKEPIKAIPAPTEGAPKQGESNLKLEGPTNAAK
                     PASADRHA"
     gene            3538..4428
                     /locus_tag="VFB80_08435"
     CDS             3538..4428
                     /locus_tag="VFB80_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33833.1"
                     /translation="MTCFLRAVFCLLALLAPGCAMLGRYSPTAGLEQRGIFQPSKYPA
                     GDWNPTAVLVQDANFTAEDGTRLHGWYVRHPQPRGQALLLHGNAGNVTLLAETLRVLN
                     RRHNLAVLALDYRGYGRSEGTPTEQGLYQDARAARRWLAHTENVAESDIILMGFSLGG
                     AVAIDLAAKDGARGLILANSFTSLPDVAQHHWPWLPMKLVVSTQMNSLEKIKQYRGPL
                     LLSHGDADEVVPYQQGVELFAAAPGPKRLITATGAKHNDPQPEEYRVAFDQFLGELPP
                     LGQSAIRPAALEVSAEAAGP"
     gene            4595..8383
                     /locus_tag="VFB80_08440"
     CDS             4595..8383
                     /locus_tag="VFB80_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN33834.1"
                     /translation="MNGWIRPLALLVIVVAAGGSAAQEAQWIWSPQHTKDQVPPGEAC
                     YFRKTISLRNPEAGSITLAADDQFELFVNGRRLASGETTRRMAEVDLSRILVRGVNII
                     AVRVQNRTGSTAALAARVQIQEGGQWQSHSTGESWKTSLRALPLWNTALYNDRSWEAA
                     QLFGELGATVPWDRRDDVPVEQVARRERFTIDSQFEVQQVLAGEQLGSLIAMSFNEFG
                     HILAAKEGGGLLLVYDGNGDKIPEKVRTYCDKVQNIQGILALNGDVFVTGVGPDGHGL
                     YRLTDKDRDGILEDVRTLVRFKCTVGEHGPHGLVLGPDGLIYVVLGNHTQLEGDYESG
                     SPYRDFYDTDLVPKYEDPSGHAAGIKAPGGTIVRTDTEGSGVQLVAGGLRNPYDLAFT
                     RDGDLFIHDADMESDEGTSWYRPTRLCHVVPGGDYGWRSGWSKWPDYFVDSLPAALDT
                     GRGSPAGIVAYNHHTFPLRYHGALFTADWSQGRILAVRLKRNGASFTASSEVFLEGNP
                     LNVTDLEVGPDGWLYFVTGGRGTGGGIYRVVWKGQVPKDVAEIGTGLTAVIRQPQPGS
                     SWARQSIAAVRKQLGVNWDNSLVGVARTAANPAHYRLQALDLMQLYGPTPTPDLLIEL
                     SREKNEIVRGRSAELMGMHATRQTHQRLIEMLDDGDRAVRRKACEALARADQAPPLDK
                     VLMLISSDDRFEAWAARRILERIDSAQWRERVLTAKSHRLLIEGCLALVVAEPTRENS
                     IAVLESLSKAMGGFVSDRDFVDLLRVAQVALVRGNLRPDELPGLKRLLAEEFPSGDAL
                     MNRELIRILAFLQESSIAERYLTYLKSDAQDIDKLHVAMYLRFIESGWQSEQRLGLLE
                     YYEDANKRKGGGSYARYILNATRDLCQQLTPEESLLVLQQGEKWPNAALGALYKVPEQ
                     LDDTTRSALISLDGKLATVQSDSVQRLQVGIVAVLARSSDPQSLAYLRKVWDQFPERR
                     QAAALGLSQQPGGENWAYLLKSLPILEPAAAREICAQLTHVEQAPAEAEPYRQAILLG
                     LKMLQKEPEKENSAANALGLLEYWTGEELAAGQPEAQRLEAWQKWFAQKYPNELEAKL
                     PVVAQTAKYTVEELSEYLASDQAKGTANRGADVFIKAQCAKCHKFEGRGEPLGPDLTA
                     VASRFTRKELLESLIHPSHVISSQYAAKTILTTDGRQFTGLVLPGGAGESIIVQPSGE
                     KMTLIARQIEAIKASKLSPMPDGLLDPLSIEEIADLIAYLQAPSAASLTRRPVGLERQ
                     "
     gene            8474..9280
                     /locus_tag="VFB80_08445"
     CDS             8474..9280
                     /locus_tag="VFB80_08445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN33835.1"
                     /translation="MTSAHNRRAWDDRAAKGQRFTQPARDQDFLDPLGKVDQLGWLGG
                     DIRGKSLLCLAAGGGKHGPLYAAAGAAVTVVDLSPAMLELDRQVAAERRLPLRTVEAS
                     MEDLSMLAAAEFDIVIHPVSTCYVPDIAPVYREVARVTRGGGLYISQHKSPTSLQAAV
                     EPSARGFELTEPYYREGPLPTVVGSPHREEGTLEYLHRWEDLLGLLCRAGFVIEDFVE
                     PLHARLDAQYGSFEHRSRWIAPYLRLKARRSGSGASPPAAKAIVLQTLTD"
BASE COUNT         1666 a         3240 c         3040 g         1656 t
ORIGIN      
        1 aggccgccct gatagaacag atcgacgatc agcttcagct cgtgcatgca ctcgaagtag
       61 gccatctccg gctgatagcc ggcctcgacc agcgtatcga agcccgcctt caccagctcg
      121 ctgacgccgc cgcagagcac gcactgctcg ccgaacaggt cggtctcggt ctcttcggca
      181 atcgtcgtct gaatcacgcc gccgcgcgtg ccgccgatgc ccttggcgta ggccaggccg
      241 accttgagcg tgctctcgct gctgccggga tagatggcaa tcaggcaggg cacgccgccg
      301 cccttttcaa attcgctgcg gaccaggtgc ccggggcctt tgggggcgac gagcatcgca
      361 tcgacgcccg gcggcggctc gacctggccg aagtgcatgt tgaagccgtg cgaggccatc
      421 aggaccgcgc cctttttcag gttcgggcgg atcgaggttc ggtagacgtc gccctgcacc
      481 tcgtcgggca gcaggatatt gacgatgtcg gcctgtttcg tggcgtcctc ggccgccagc
      541 ggcttgaagc cgtgcttgac ggccagctca tagttcgggc tgccgggccg ctggccgacg
      601 atcacgctca cgccgctgtc gcgcaggttc tgagcctggg cgtgtccctg gctgccgtag
      661 ccgaggatgg cgatcgtctt gcctttgagg acggagaggt cggcgtcgtt gtcgtaatag
      721 atcgtggcgg gcatcggagg gaatttcaga tttcagattg cagatttcag cacgctaacc
      781 ggcgaacttc ttcagcgcgg cgtcttgctg ctcggcctct tcctcggtct tgggctggcg
      841 gtacttgccg cggaccatgg cgatgcggcc ggtgcggacc agctccagga tgccgtaggg
      901 acgcatccgc tggatgaagg cctcgatctt gctctcgcgg ccggaaatct cgatcatcac
      961 ttcttcggcc cccacatcga cgatctgggc ccggaagatc tccaccagct cgcggacctc
     1021 ggagcgcttc tggcccggcg gggcctcgac cttgagcagc atcaggtccc gctcgacgtg
     1081 gtcgcgcgag ctgatgtcgt ccacctgcac cacggtcacg atcttctcga gctgcttgcg
     1141 gacctggtcg agcacgcggt cgtcgccgac gaccacgaag gtcattcgcg agaggttggg
     1201 gtcctccgtt tcgcccaccg ccagcgaatc gatgttgtag ccgcgggagg cgagcatgcc
     1261 cgagatgtgc gcaagcacgc cgggcacgtt ttgcacgacc gcggagagga cgtgacgcat
     1321 gacagaactc cagccaaacg ggtcatccta gtggccattt gccgaggcga caaggacgca
     1381 gcggcggcca gaaaacgacg caggccggga caatcgcccg gcccgcagag ttcgcctgat
     1441 aggtcagttc gctaggcgtg gcgatcggcg ctggccggct tggccgcgtt cgtcggacct
     1501 tccagcttca ggttcgactc gccctgcttg ggagcaccct cggtcggcgc gggaatagcc
     1561 ttgattggct ctttcttctc ggcattggcg ggcttgctgg cgtcgccggc gggcgtgcct
     1621 tccgttttct tctcttccgg ttgcgtgccg gcgggcttgg gctcgcccgc ggggggtgtc
     1681 ccgttggtct tcttgtccgt cgcgggcgtg ccgctgttga ccggcggctt gttgacgtcc
     1741 gtcctcgggg gcagtgcagc cggcggcagt gcagcgggcg gggccgcggg cggcgctggc
     1801 gggatcggcg tgctgcggta ataggtcgtc gtcggcccgt agaccgcgtc gccataataa
     1861 gaagtcagcg gcacgcgata ggtctcgatc cgcggctgca agcgcatgga agtctgagcg
     1921 acccacttgg ccacggtccg cggcacctgc acgctgcgcg tctcgttcac atagcggcag
     1981 accttgatcg gcgtctgctc gacgacttct tcgtattcga ttcgctgcac ggtgtaagga
     2041 atctggcgga cctgcacttc ctcttcccag cgcatcttct gaaccggaac ctgataggtc
     2101 acgcgctcgg tcacgggttt ttggaccgtg aacggaacgt cgcggacctc ctcataggtt
     2161 tccatccgct gcacggtgat cgggacctgc tgcgtctgca attcgtcgta gtagcggttc
     2221 accgtaacgg gaacctgctg cgtcatctgc accggctgat agctggtcgt cgcgacctgc
     2281 tgggcgacga tgttcggcat gtacgccgtc acggggcgca gcacgctcgg ccctggcgtg
     2341 ggcacccatc gcaggccacc gcgatagtag gtcgtctggc ccgtcgccgg gtccgcggca
     2401 tagccgctcg gaacgcacca cagccgatta cggacgaccg gcgccgtggg ctgataggcc
     2461 gtcacatact gcccctgatc gacatactgc gtgtgcatcg acgtcacagg ttgataggct
     2521 gtgtaagcct gctcctgcat gacggtctct tgataggcct tgcggacggt atattgctgc
     2581 tcccgcatct gcgtctcgac gacggggcgc tggacttgat agcgctcctg gcgggtttgg
     2641 gtttcagtca catacgagac cttgtcataa ctctcttccc gatactcggt ctcatacacg
     2701 ggcttgcgga gcagcacccg ttcttcacgc gtcgccgtct cggtcaccgg cttggcgacg
     2761 gtatagcgcc gctcgcgcag ctcgtcggtc cactgcgggc gtagcgtggt gacttgctga
     2821 tcttccagca cggtctcata ttccagccgc cacgttgtca cgggcacctg gtcatagacg
     2881 accttgttga cgatgcgaaa cgacgggcag ccacagccgc actgggcctg ggctgagggg
     2941 gccagcaaca ggacgcagcc gctgacggcg agcgccatcc aggaacggat tgtgacagtc
     3001 ataaggcctc tcacgctcac cgcaggggtg ctttctcaaa ggggcgccac ggccaagagc
     3061 ttcctctccc ttggcctgcg gcaaacaacc gcgcaatcac tcgcgccgcg ttacacaact
     3121 aagaaatgtg ccggatgcgg gttcccaatg caaacagatt cttgatgcct ccgcggccag
     3181 atgtgcgtta actgcttcag tctcacgcag ttgcgccgcg aaaaattttt tgaagaaacg
     3241 ccggcccgct gccgacaggc tgtggcaccg gttttcccca tgtatatgac tctcatatgg
     3301 ttgcgccgca agtttgcgca agctgcgtaa ccggaataag tggaacttga gtctccagcg
     3361 cagcgctgag gcggttgggc gccattcttc gctcgcccct gccgcgggtc aattctcact
     3421 cacttgccta cgcatttctt gagcgaaagt tcccggaata ttgaaacgac attgacgctg
     3481 ctagatgagt caaatactct ccgcggccct gtctccaggc actagcgggc cgctgtcatg
     3541 acttgctttc tgcgcgctgt cttctgcctg ctcgcgctgc tagcgcccgg ctgcgccatg
     3601 ctgggaaggt actcgccgac cgccggcctg gagcagcgcg gcatctttca gccgtcgaag
     3661 tatccggcgg gtgattggaa cccgacggcg gtcctggtgc aggatgccaa tttcacagcg
     3721 gaagacggca ccagactgca cggctggtac gtgcggcatc cgcagccgcg ggggcaggcc
     3781 ctgctgctgc acggcaacgc cggcaatgtg acgctactgg ccgagacgct gcgggtgctc
     3841 aaccggcggc ataacctggc ggtgttggcc ctggactatc gcggctacgg ccgcagcgaa
     3901 ggaacgccaa ccgagcaagg actctatcaa gacgcgcggg cggcccgccg ctggctcgcc
     3961 catacggaaa acgtcgccga atccgacatc atcctcatgg gcttctcgct cggcggcgca
     4021 gtggcgatcg acctggccgc caaggatgga gcccgaggcc tcattctggc caacagcttc
     4081 acttcgctcc ccgacgtcgc ccagcatcat tggccgtggc tgcccatgaa gctcgttgtc
     4141 tccacgcaga tgaactcgct ggagaagatc aagcagtatc gcgggccgct gctgctcagc
     4201 cacggcgacg ccgacgaggt cgtgccgtat cagcagggcg tggagctgtt tgccgccgcg
     4261 cccggcccga agcggctgat caccgccacc ggcgccaagc acaacgatcc gcagccggag
     4321 gaatatcgcg tggccttcga ccagttcctc ggcgagctgc cgccgctggg gcagtcggca
     4381 atccggccgg ctgcgctgga agtaagcgcc gaggcagccg ggccctagcc agcagccggc
     4441 acggcctagc tagcagccgc caatttctcc cttgccccgt ttggcggctg tttgctaagg
     4501 ttcgcgccga ttgcagcgcc gcgcttgcgg catgccgcac tgtttgcctg ccccatgaag
     4561 gaggcgggcc cgatcagcct tggcagaggt tgccatgaac ggatggatcc gaccgctggc
     4621 cctgctcgtg attgtcgtcg ccgctggcgg cagcgccgct caggaagcgc agtggatctg
     4681 gtcgccacag cacaccaagg accaggtgcc gccgggcgaa gcgtgttatt ttcgcaagac
     4741 aatttcgctg cgcaaccccg aagcgggcag catcacgctg gctgccgacg atcagttcga
     4801 gctgttcgtc aacggccgcc gcctcgcctc gggcgagacc acgcgcagaa tggctgaggt
     4861 ggacctgtcc cgcatcctgg tgcgcggcgt caacatcatc gccgtccgcg tgcagaaccg
     4921 gaccggcagc acggcggccc tggccgcccg cgtccagatc caggaaggcg gccagtggca
     4981 gtcgcactcc acgggcgaga gctggaagac gtcgctccgc gcgctgccgc tgtggaacac
     5041 ggccctctac aacgaccgca gctgggaggc ggcccagctc ttcggcgaac tcggcgcaac
     5101 cgtcccctgg gaccgccgcg acgacgtgcc cgtcgaacag gtcgcccgcc gagaacgctt
     5161 caccatcgac tcgcaattcg aagtccagca ggttctggct ggcgaacaac tcggatcgct
     5221 gatcgccatg tcgttcaacg aattcggcca catcctcgca gccaaagagg gtggcggcct
     5281 gctcctggtc tacgacggca acggcgacaa gattcccgaa aaggtccgca cctactgcga
     5341 caaggtgcag aacatccagg gcatcctggc cctcaacggc gacgtgttcg tcaccggcgt
     5401 tggccccgac ggccacggcc tctatcgact gaccgacaag gatcgcgacg gcatcctgga
     5461 agacgtccgg acgctggtcc gcttcaagtg caccgtcggc gagcacggcc cgcacggcct
     5521 ggtgctcggc cccgacgggc tgatctacgt cgtgctcggc aaccacacgc agctcgaagg
     5581 cgattacgaa agcggcagcc cctaccgcga tttctacgac accgacctgg tgccgaagta
     5641 cgaagacccc agcggccacg ccgccggcat caaggccccc ggcggaacga tcgtccgcac
     5701 cgacaccgaa ggcagcggcg tgcagctcgt ggccggcggc ctgcggaatc cctacgacct
     5761 ggcgttcacg cgcgacggcg acctcttcat ccacgacgcc gacatggagt ccgacgaagg
     5821 aacctcctgg taccgcccca cgcggctgtg ccacgtcgtc cccggcggcg attacggctg
     5881 gcgcagcggc tggtcgaagt ggcccgatta cttcgtcgat tcgctgcccg cggcgctcga
     5941 caccggccgc ggctcgcccg cgggaatcgt cgcctacaac caccacacgt ttccgctccg
     6001 ctatcacggc gcgctgttca cggccgactg gtcgcagggg cgaatcctcg ccgtgcggct
     6061 gaagcgcaac ggagcgagct tcaccgccag cagcgaagtc ttcctggaag gcaacccgct
     6121 caatgtcacc gacctggaag tcggccccga cggctggctc tatttcgtca ccggcggccg
     6181 cggcaccggc ggcggcatct accgcgtcgt ctggaagggg caggtgccca aggacgtcgc
     6241 cgagatcggc accggcctga ctgccgtgat ccggcagccg caacccggca gcagctgggc
     6301 ccggcagtcg atcgcggccg ttcgcaagca gctcggagtg aattgggaca acagcctggt
     6361 cggcgtcgcg cggacggcgg ccaatccggc gcactaccgc ctgcaggccc tcgacctgat
     6421 gcagctgtac ggcccgacgc cgactcccga cctgctgatc gagctgtcgc gcgagaagaa
     6481 cgaaatcgtc cgcggacgct ccgccgagct gatgggcatg cacgccacgc ggcagacgca
     6541 tcagcggctg atcgaaatgc tggacgacgg cgaccgcgcg gtccgccgca aggcctgcga
     6601 agcgctggcc cgcgccgacc aggcgccgcc gctggacaag gtgctgatgc tgatcagctc
     6661 cgacgaccgc ttcgaagcct gggccgcccg gcggattctg gaacggatcg actcggccca
     6721 gtggcgcgag cgggtgctga ccgccaagag ccaccgcctg ctcatcgaag gctgcctggc
     6781 gctggtcgtc gccgagccga cgcgcgaaaa ctccatcgcc gtgctcgaat cgctcagcaa
     6841 ggcgatgggc ggcttcgtca gcgaccgcga cttcgtggac ctgttgcgcg tcgcgcaggt
     6901 ggctctcgtc cgcggcaatc tgcggcccga cgagctgccc ggcctgaagc ggctcctcgc
     6961 ggaggaattc ccctcgggcg acgccctgat gaaccgcgag ctgatccgca tcctggcgtt
     7021 cctccaggaa tcgtcgatcg ccgagcgcta cctgacctac ctcaaatcgg acgcccagga
     7081 catcgacaag ctgcacgtgg cgatgtacct gcggttcatc gaatcggggt ggcagtccga
     7141 gcagcggctg ggcctcttgg aatactacga agacgccaac aagcgcaaag ggggcggcag
     7201 ctacgcccgc tacatcctca acgcgacccg cgacctctgc cagcaactga cgccggaaga
     7261 gtcgctgctc gtgctgcagc aaggcgagaa atggccgaac gccgcgctgg gcgcgctcta
     7321 taaagtgccg gagcagctcg acgacacgac gcgctccgct ttgatctcgc tcgacggcaa
     7381 gctggccacg gtccagagcg actcggtcca gcggctgcaa gttggcatcg tggccgtgct
     7441 ggcccgcagc agcgacccgc aatcgctggc ctacttgcgg aaagtctggg accagttccc
     7501 cgagcggcgg caggccgcag cgctgggcct ctcgcagcaa cccggcggcg agaactgggc
     7561 ctacctgctc aagtcgctgc cgatcctgga gcccgccgcg gcccgcgaaa tctgcgccca
     7621 gctgacgcac gtcgaacagg cgccggccga ggcggagccc tatcgccagg cgatcttgct
     7681 gggcctgaag atgctgcaga aagagccgga aaaggagaac tccgctgcca acgcgctggg
     7741 cctgctcgaa tactggacgg gcgaagagct ggccgccggg cagccggaag cccagcggct
     7801 ggaggcgtgg cagaagtggt tcgcccaaaa atatccgaat gaactggaag ccaaactgcc
     7861 cgtggtggcc cagaccgcga agtacaccgt ggaggagctg agcgaatacc tggccagcga
     7921 ccaggccaag ggcacagcca accgcggggc cgacgtgttt atcaaagcgc agtgcgccaa
     7981 gtgccacaag ttcgaaggcc gcggcgaacc gctggggccg gacctgaccg ctgtcgccag
     8041 ccgcttcacg cgcaaggagc tgctggagtc gctcattcac ccctcgcacg tcatttcgtc
     8101 gcaatacgcg gccaagacga tcctcacgac cgacggccgg caattcacgg gcctcgtgct
     8161 gccaggcggc gcgggtgaat cgatcatcgt gcagcccagc ggcgaaaaaa tgacgctcat
     8221 cgcgcggcag atcgaggcca tcaaggccag caagctctcg ccgatgcccg acggcctgct
     8281 cgatccgctt tcgatcgagg agatcgccga cctgatcgcc tatctgcagg ccccctccgc
     8341 cgcctcgctc acccggcggc ccgtggggtt ggagagacag tagagccagg aaccagtagc
     8401 cagtagccag tagccagggg ccagtagcca agtaaattgg tttctggctc ctggtccctg
     8461 gcccctggct cctatgacct ccgcccacaa tcgccgcgct tgggacgatc gcgcggccaa
     8521 ggggcagcgc ttcacgcagc cggcccgcga ccaggatttt ctcgacccgc tgggcaaggt
     8581 cgatcagctc ggctggctgg gaggagacat tcgcggcaag tcgctgctct gcctggcggc
     8641 cggcggcggc aagcacggcc cgctctatgc cgcggccggc gcggccgtga ccgtcgtcga
     8701 tctcagcccc gcgatgctgg agctcgaccg ccaggtcgcc gccgaacgcc ggctgccgct
     8761 gcggacggtc gaagcctcga tggaagacct ctcgatgctg gctgcggcgg agttcgacat
     8821 cgtcattcat cccgtcagca cctgctacgt gccggacatc gcgcccgtct atcgcgaagt
     8881 cgcccgcgtc acccgcggcg gcggcctgta catcagccag cacaagtcgc ccaccagctt
     8941 gcaggcggcc gtcgagccct ccgcccgcgg cttcgagctg accgagccct actatcgcga
     9001 aggcccgctg cccaccgtcg tcggcagccc gcaccgcgaa gagggcacgc tcgaatatct
     9061 ccaccgctgg gaagacctct tgggcctctt gtgccgcgcc ggcttcgtca tcgaggactt
     9121 cgtcgagccc ctccacgccc gcctcgacgc ccagtacggc agcttcgagc accgcagccg
     9181 ctggatcgcc ccctacctcc gcctcaaagc ccgccgcagc ggctccgggg caagtccgcc
     9241 cgctgcgaag gcgatcgtgc tgcaaacttt gacagactaa tgaagcctga ccccttttat
     9301 cttctgaccc cttttatctt ctcaaaagag aagatcaaca agtccaatcc aagcaatgct
     9361 ccttaatttg taatgcagac gaaactgtgg ctgacctcac ctatacgtat gtaactgaac
     9421 agatggacca tgaagaccaa tccttaccat gtcgccgaac acggagataa tccgccgcaa
     9481 gggcgcacat cattgctgct gacacgcatt ttatggtttg cgatcggcgc ggttgtttca
     9541 tgcggagtct ggtcgatggt caacttcaca gatcgaaggc cgcgcgacta caccgtcgaa
     9601 tg
//
LOCUS       DASRJB010000314         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2346877, whole genome shotgun sequence.
ACCESSION   DASRJB010000314 DASRJB010000000
VERSION     DASRJB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346877"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VFB80_08450"
     CDS             complement(<1..1490)
                     /locus_tag="VFB80_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HZN33836.1"
                     /translation="MYTPASTIVEILCRRIETDGPQPALAVKRGGQYQWHTWNDLAGE
                     VARLSAVLVALGVRPGDRVAHLSENRYEWILADWAIQMAGAIHVPIHAPLTGVQAAWQ
                     VRHSGANVLLLSGPAQAAKLAAPAVEPIAGIQCVAYDACQEPLWGRRIATLSELQAAA
                     DLQKGIDAACRTRKEIRPESPVTILYTSGTTGEPKGVMLTQGNLATNCCATIEAFGER
                     QNQLRLNFLPLSHIFARTCDLYLWVADGSQLALAEARETVIADCQAVHPTMLNGVPYF
                     FDKVYRGLCEKGVQSIPGVLKGALGGAIQNCCAGGAALPDHLYDYYHSQGVPLVQGYG
                     LTETSPVITISTEQHHRRGSCGRAIPGVEVKIAADGEILTRGPHVMAGYYENPAATAE
                     VLQGGWLATGDLGRLDADGFLYITGRKKEILVTSGGKNIAPVFLESLLTQDPLILQAM
                     VVGDGRSHLGALIIPNRDLVKAELARGTAADEQALIRARIDACLANV"
     gene            1581..>3453
                     /locus_tag="VFB80_08455"
     CDS             1581..>3453
                     /locus_tag="VFB80_08455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN33837.1"
                     /translation="MATSPPPTESVTEASFAETALKLGGKSEEEARRMGAVDRADDQV
                     ERLFQPQYQTTSSPIHRAVWERMLPVELFAAAEEKTPGDVQTVMSRSLDVVRRHRDAK
                     SLHNPDGKIAEGVLADLAGAGYWGLLVDREYGGSGAPLRSFMPFLTQMALIDPTIAGL
                     ASVHGCIGAVDPVRTFGNDEQKRRFLPKLASGERLSAFALTEPGAGSDLTALRTRARL
                     VGNQYLVTGEKLFITNVTPGRTIGLVCLIDDKPAVLIADLPPQESEQFQLVKYGLYAL
                     KHTYNRGIVFRDFPVPAENLLQVPKGNGLTIAYHGLNLGRVALCATAAGNMRLLMASM
                     IPWANFRRTYGEAIARRELVRRRLARLAGLIVACDALVAWCSTLLDQGYRGEMECIIA
                     KIFGSEAQKEAAIELFMKTHGGRSFLHGHLLGDNVHEYLAPCIYEGEGEMLGMALFKS
                     LVKQHGTEFFEPVGKALAAAGMKTFSPANPAHVWKLRQVLVPYASWTLREMFASKSHS
                     ELGNLPPQLREHALFAIRGLQRMPLEISEAMRKHQLKLADRQCRIAELSQRVQDTVVM
                     LVTALWAHQRKNEATTQAADVLCQDMTRKLTGARPSDAYFKQVSELADAVQKGGFEEI
                     "
BASE COUNT          569 a         1143 c         1176 g          565 t
ORIGIN      
        1 acgttggcca ggcaggcgtc gatccgtgcg cgaataagcg cttgctcgtc ggccgccgta
       61 ccgcgggcca gttccgcttt cacgaggtcg cgattcggga taatcagcgc ccccaggtgc
      121 gaccgcccgt cgccgacgac catcgcctgc aagatcagtg ggtcctgcgt cagcagcgat
      181 tcgaggaaca ccggcgcgat gttctttccg ccggacgtga cgaggatttc cttcttgcgg
      241 ccggtgatgt agagaaagcc gtcggcatcg agccggccca ggtccccggt ggcgagccag
      301 ccgccctgca agacttcggc ggtcgcggcc ggattctcgt agtacccggc catgacgtgc
      361 gggccgcgcg tgaggatttc gccgtcggcg gcgatcttca cttcgacgcc gggaatggcg
      421 cgcccgcatg agccgcggcg gtggtgctgc tcggtcgaaa tcgtgatgac gggcgacgtc
      481 tcggtaaggc cgtagccctg caccagcggc acgccctggc tgtggtaata gtcgtacaaa
      541 tggtcgggca gggccgcgcc gccggcgcag cagttttgaa tggcgccgcc cagcgctccc
      601 ttgagcacgc ccggaatgct ctgcacgccc ttttcgcaca acccgcgata gaccttgtcg
      661 aaaaagtacg gcacgccgtt gagcatggtc ggatgaaccg cctggcaatc ggcgatcacg
      721 gtttcgcggg cctcggccag cgctagctgc gagccgtcgg ccacccacag gtacaggtca
      781 caggtgcggg cgaagatgtg gctcagcggc aggaagttca gccgcagctg gttctgccgc
      841 tcgccaaagg cctcgatcgt cgcgcagcaa ttcgtcgcca ggttgccctg cgtaagcatc
      901 acgcccttcg gctcgccagt cgtgccggac gtgtagagga tcgtcaccgg cgattcgggg
      961 cggatctcct tccgcgtccg gcaggcagcg tcgatgccct tctgcaagtc ggccgcggct
     1021 tgcaattccg agagcgtggc gatccgccgg ccccagagcg gctcttggca agcgtcgtag
     1081 gcgacgcact ggatgccggc gatcggctcg acggccggcg cggccagttt cgctgcttga
     1141 gcggggccgg acagcagcag cacgttcgcc ccgctgtgcc gcacctgcca ggcagcttgc
     1201 actcccgtca gcggtgcatg gatcggcacg tgaatcgccc cggccatctg gatggcccag
     1261 tcggcgagaa tccactcgta gcggttttcc gacaggtggg ccacgcggtc gcccggccgc
     1321 acgcccagag cgaccagcac ggccgacagc cgggccactt cgcccgccag gtcgttccag
     1381 gtgtgccact ggtattgccc gccgcgcttc actgccaggg ccggctgcgg gccgtcggtc
     1441 tcgattcgcc ggcagagaat ctcgacgatc gtggaagcgg gcgtgtacat gggaccgatt
     1501 atggcaggtt ttcgggctgc cgcgagcagt ttgcctcagg cggtcgtatc caattggagc
     1561 ccgcgggcta gaattgctgc atggccacct ctcctccgcc gaccgaatcc gtcaccgagg
     1621 cctcctttgc cgaaacggcc ctgaagctgg gcggcaagag cgaggaagaa gcccgccgca
     1681 tgggcgcggt ggaccgggcc gacgatcagg tcgagcggct gtttcagccc cagtatcaga
     1741 cgaccagcag cccgatccac cgggcggtct gggagcggat gctgcccgtc gagctgttcg
     1801 ccgccgccga ggagaaaacg ccgggcgacg tgcagacagt gatgagtcgc tctctcgacg
     1861 tcgtgcgtcg gcatcgcgac gcaaagtcgc tgcacaatcc cgacggcaag atcgccgagg
     1921 gggtgctggc cgacctggcg ggcgccggtt attgggggct gctcgtcgat cgcgaatatg
     1981 gcggcagcgg cgcccccctg cgcagcttca tgccgttcct gacgcagatg gcgctcatcg
     2041 atccgacgat cgccggcctg gcgtcggtcc acggctgcat cggcgcggtc gatccggtcc
     2101 gcaccttcgg caatgacgag cagaagcggc ggttcctgcc caagctggcc agcggcgagc
     2161 gcttgagcgc gtttgccttg accgagcccg gtgcgggctc cgacctgacg gcgctgcgga
     2221 cgcgggctcg cctggtcgga aaccagtatc tcgtcacggg cgaaaagctg ttcatcacga
     2281 acgtcactcc cggccgcacg atcggcctgg tctgtctgat cgacgacaag ccggccgtgc
     2341 tgatcgccga tctgccgccg caagagagcg agcagttcca gctggtgaaa tacggtctgt
     2401 acgccctcaa gcacacgtac aaccgcggca tcgtcttccg cgattttccg gtgccggccg
     2461 agaacctgct gcaagtgccc aaggggaacg gcctgacaat cgcgtatcac ggcctcaacc
     2521 tgggccgcgt agcgctctgc gcgacggcgg cgggcaacat gcggctcttg atggcgagca
     2581 tgatcccctg ggccaatttc cgccgcacgt acggcgaggc gatcgcccgc cgcgagctgg
     2641 tccgccggcg gctggcccgg ctggccggcc tgatcgtcgc ctgcgacgcg ctggtcgcct
     2701 ggtgctcgac gcttttggac cagggctacc gcggcgaaat ggagtgcatc atcgccaaga
     2761 tttttggcag cgaggcccag aaggaggccg cgatcgagct gttcatgaag acgcacggcg
     2821 gccgctcgtt cctgcacggc cacctgctgg gcgacaacgt tcacgagtac ctcgcgccgt
     2881 gcatctacga aggcgaaggc gagatgctgg gcatggcgct gttcaagtcg ctggtcaagc
     2941 agcacggcac ggagttcttc gagccggtcg gcaaggccct ggccgcggcg ggaatgaaga
     3001 cgttcagccc cgccaacccg gctcacgtct ggaaattgcg ccaggtgctt gtgccgtacg
     3061 ccagttggac gctgcgcgaa atgttcgcga gcaaaagcca cagcgagctg ggcaatctgc
     3121 ccccgcagct tcgcgagcac gccctgttcg cgatccgcgg cctgcagcgg atgccgctgg
     3181 agatcagcga ggcgatgcgc aagcaccagc tcaagctggc cgaccgccag tgccgcatcg
     3241 ccgagctgtc gcagcgggtg caggacacgg tcgtgatgct ggtgacggcg ctgtgggcgc
     3301 accagcgcaa gaatgaggcc accacgcaag cggccgatgt cctgtgccag gacatgacgc
     3361 gcaagctgac cggcgcgcgg ccgagcgacg cctacttcaa gcaggtctcg gagctggccg
     3421 acgcggtgca gaagggcggc ttcgaggaga tcg
//
LOCUS       DASRJB010000315         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4038299, whole genome shotgun sequence.
ACCESSION   DASRJB010000315 DASRJB010000000
VERSION     DASRJB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038299"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2254
                     /locus_tag="VFB80_08460"
     CDS             <1..2254
                     /locus_tag="VFB80_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN33838.1"
                     /translation="DAARLVTSLDPEAAPGVAPYVNDKALLTSLPVAVQLTLGEFPQL
                     RQSLGDRFAALARLRIGQAIAAGDAATIELATVQFAGTDAAAEAHQWLGDRALAGGAF
                     TRAILEYTRAGQSDASLASGISPRIRLAAAMLGRDEQAAVTRPVRFGELALSAAEFEA
                     LVTEMRSRGQSSALAVSPQITAARAAPAPSGFDVHVRSKLDGPTGDRPQEEVGRRTNQ
                     YRVPWADRQIATAVEGDTLYVANRFQVAAYNLADGQRKWQSQNPPGSMARAQDWAMIP
                     MQPLITGDRIFVRLLYSSNPLLVCLEKSTGKLLWVAENRDREYLVSDPHLVQGQLVAL
                     GVQIQQEQQGQLRWCQFDPQTGEMLKQRDLVRLRNTWGTRSCCEIAPLDDGLVAALGG
                     LTLAVDAAGNIRWVRTHVTLPADEDPRWVLQSYQPPLIQGDRLYVAQPGARTVECLSA
                     ASGRKFWSAVLPEVIGIAGLSGNRLIVRTEAGLRALDVADGKTLWRYAASELYSFPLV
                     DEKSLLVASRERVVNQNDKWQVRLTWLDPATGQPAATTVLAKLSESDPRLGPLVPYKD
                     RLFTFFGRGQHDPVREVVELVPKGKADRPPPIEAASDPWRQRLPGELTQAAWELLGDW
                     QLISAQGGDRTGRVAEVHGERDVLGLRASGAWPVVLARELTIPKQGRPRLRLKLGNDG
                     GQLWKLEVRHGERLLKTEEFKDETHKDRWKTLEIDLTPAAGHAGWLTIDARSANGDHV
                     LWWKDAEVLF"
     gene            2251..3123
                     /locus_tag="VFB80_08465"
     CDS             2251..3123
                     /locus_tag="VFB80_08465"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZN33839.1"
                     /translation="MKGRAASSLLGAHMSIAGGYYKAVEEARRCGCDVVQLFTKNNTQ
                     WRAKPITDDEARQFGSALTELKIGSPISHASYLINLASPDEALRSKSIDGMAVELARA
                     EQLGIPFVIVHPGAHTTATEEEGLALVSASIDEIHCRLPQAKTEISLELTAGQGSCLG
                     WKLEQLAAIIRNSKQPDKLRICVDTCHAYAAGYDLRDRKGYLAFWRQFDELLGLPRLV
                     AMHLNDSKRELGSRVDRHEHIGRGQLGPEAFRQLLRDKRFRHVPMYIETAKGQENGES
                     LDIINLRVLRELAR"
     gene            complement(3220..4578)
                     /locus_tag="VFB80_08470"
     CDS             complement(3220..4578)
                     /locus_tag="VFB80_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33840.1"
                     /translation="MIRTLLSFCLLFGGLVAVSKWLHDATCPAAGDAMPADSSAEPAP
                     APPAAAKPSRLPSSGEAQPAKVRLVAFEDDEPDAPKKDNPVGSTPKTEPTEGDGDQTP
                     PSVPSAKRDIVEIELTSELEELRGRIRECLAYYYFRPENVAQRSPWGAMHAMIAYGVD
                     SELIAGDKRVNAVGYLCYNGVCNGQQLFYLSNGKLQTRIGPGVQGHPGQFLAMLAQSR
                     VKTTFPLVVEGKQFTVADLIQHEKDTCRPKTELTFKLIAFTHYLKSDEKWKSNDGQDW
                     DIPRVIKEELAQPIVGAACGGTHRMTGFSYAYRKRETRGEDFTSQWLRAKTFVEDYHN
                     YAFKLQNPDGSFSTDWFAGRADFGPPTRRLETTGHITEWLTYSLTRQQLIEPRMTKSV
                     SYLTNLLLNNRNEKWGIGPLGHGLHALAMFDERVFGGQPGTRAVELAEVKKPQEKKAV
                     ER"
BASE COUNT          805 a         1518 c         1557 g          798 t
ORIGIN      
        1 cgacgccgcc cggctggtaa cctcactcga tcccgaggcg gccccgggcg tcgctccgta
       61 tgtcaacgac aaggccctgc tcacatcgct gccggtggcc gtgcagctga cgctgggcga
      121 atttccgcag ctgcggcagt cgctgggcga ccgtttcgcg gcgctggccc ggctgcggat
      181 cggccaggcg atcgccgcgg gcgatgcggc gacgatcgag ctggcgacag tacagttcgc
      241 cgggacggac gccgcggcgg aggctcacca atggctcggc gatcgagcgc tggccggcgg
      301 tgcattcacg cgggcgatcc tggagtacac gcgggctggc caaagcgacg cgtcgctcgc
      361 gtccggcatc tcgccgcgga ttcgactggc ggcggcgatg ctggggcgcg acgagcaggc
      421 ggcagtgacc cggccggtgc ggttcggcga gctggcactt tcggcagccg aattcgaggc
      481 gctcgtgacc gagatgcgca gccgcgggca gtcgtcggcg ctggccgttt cgccgcagat
      541 cacggcggcc cgtgcggcgc ctgctcccag cggcttcgat gtgcacgtcc gcagcaagct
      601 cgacgggccg accggcgacc gcccgcagga agaagtcggc cgccgcacga atcaataccg
      661 cgtgccgtgg gccgaccggc agatcgcgac cgccgtggaa ggcgacacgc tgtatgtcgc
      721 caaccgcttc caggtcgcgg cctacaacct cgccgatggc cagcgcaaat ggcaaagcca
      781 gaatccgccg ggctcgatgg cccgcgccca ggattgggcg atgattccga tgcagccgct
      841 gattaccggc gaccggattt tcgtgcggct gctctattcc tccaatccgc tgctggtctg
      901 tttggagaaa tcgacaggca agctgctgtg ggtggccgag aaccgcgacc gcgaatacct
      961 cgtctccgac ccgcacctcg tgcaggggca actcgtcgcc ctgggtgtgc agattcagca
     1021 ggagcagcag gggcagcttc gctggtgcca gttcgatccg caaacgggcg agatgctgaa
     1081 gcagcgcgat ctcgtgcggc tgcggaacac ctggggcacg cgctcctgct gcgaaattgc
     1141 tccgctcgac gacggcctgg tcgcggcgct cggcggcttg acgctcgcgg tcgatgccgc
     1201 cggcaacatc cgctgggtcc gcacccacgt gacgctgcct gccgacgagg acccgcggtg
     1261 ggtgctgcaa tcctatcagc cgccgctgat ccagggcgat cggctgtacg tggctcagcc
     1321 gggcgcgcga accgtcgagt gcctcagcgc cgcgtcgggc cgcaaattct ggagcgccgt
     1381 gctgccggaa gtgattggaa tcgccggcct gtcaggcaat cgactcatcg ttcgcaccga
     1441 ggcaggcctg cgggcgcttg atgtcgctga cgggaagacg ctgtggcgct acgcggcgag
     1501 cgagctctac agctttccgc tggtcgatga gaaatcgctg ctggtggcca gccgcgagcg
     1561 cgtagtcaat cagaacgaca agtggcaagt ccggctcacc tggctcgatc cagcgacggg
     1621 acagcccgcg gcgacgacgg tgctggcaaa actgtccgaa agcgatccgc gcttgggtcc
     1681 gctcgtcccg tacaaggacc ggctgttcac cttcttcggc cgcggccagc acgatccggt
     1741 ccgcgaggtt gtcgagctcg tgcccaaagg caaggccgac cgcccgccgc cgatcgaggc
     1801 ggcgagcgac ccctggcgac agcgcttgcc aggcgaattg acgcaggcag cgtgggaact
     1861 gctgggcgac tggcagctga tcagcgctca aggaggcgac cgcacgggcc gcgtcgccga
     1921 agtacacggc gagcgcgacg tgctgggcct gcgcgcgagc ggcgcctggc ccgtcgtcct
     1981 ggcccgcgag ctgacgattc ccaagcaagg ccgcccgcgc ctgcggctga agcttggcaa
     2041 cgacggcggc cagctttgga agctcgaggt ccgccacggc gagcggctgc tcaagaccga
     2101 agagttcaag gacgagacgc acaaggaccg ctggaagacg ctcgagatcg acctgacccc
     2161 tgccgccggc cacgccggct ggctcacgat cgacgcccgc agcgccaatg gcgaccacgt
     2221 gctatggtgg aaggacgcag aggtgctgtt ttgaaagggc gtgctgccag ttcgctcctc
     2281 ggcgcccaca tgtcgatcgc cggcggatac tacaaggccg tggaggaggc tcggcggtgt
     2341 ggctgtgatg ttgttcagct tttcaccaag aacaacacgc agtggcgggc caagccgatc
     2401 accgacgacg aggcgcggca gtttggctcg gcgctgactg agctgaagat tggcagcccg
     2461 atttcgcacg cgtcttacct gatcaatttg gcgtcgcctg atgaagcgct gcgatcgaag
     2521 tcgatcgatg gcatggccgt ggagctggcg cgagccgagc agctaggtat tcccttcgtc
     2581 atcgtgcatc cgggagccca cacgactgct actgaagagg aagggctcgc cctggtttcc
     2641 gccagtattg acgagattca ctgccggctg ccgcaagcca aaacagagat ctcgctggag
     2701 ctgaccgccg ggcagggctc ctgcctgggc tggaaactcg agcaactggc ggcgatcatc
     2761 cggaattcga aacagcccga caagctgcgg atttgcgtcg atacctgcca cgcctacgcg
     2821 gccggctacg acctgcgcga tcgcaaaggc tatctcgcgt tctggcggca gttcgacgaa
     2881 ctgctgggac tgccgcggct ggtggcgatg cacctgaacg acagcaagcg cgagctcggc
     2941 tcgcgggtcg atcggcatga gcacattggc cgcggccaac tcggccccga ggccttccgc
     3001 cagctccttc gcgacaaacg cttccgccac gtgcccatgt acatcgaaac cgccaagggc
     3061 caggagaacg gcgaatcgct cgatatcatt aacctgcggg tgttgcggga actggcgagg
     3121 tgataagaag aaagaaccaa agaacctaag aacctaagaa ccaaagggac ggctcccatt
     3181 tggttctttg gttcttcgga acttggaaat ttcccctgcc taccgctcca ctgccttctt
     3241 ttcctgcggc tttttgacct cggcgagttc tacagctcgc gtgccgggct ggccgccgaa
     3301 gacccgctcg tcaaacatcg ccaaagcgtg caggccgtgg cccagcgggc cgatgcccca
     3361 tttctcgttg cggttgttga gcagcaggtt cgtgagatag ctgacgctct tcgtcatccg
     3421 cggctcaatg agctgctgcc tggtcagcga ataggtcagc cactcggtga tgtggcccgt
     3481 cgtctccagc cggcgggtgg gcgggccaaa gtcggcccgg ccggcgaacc agtcggtgct
     3541 gaagctgccg tcggggtttt gaagcttgaa ggcgtagttg tgatagtcct cgacgaacgt
     3601 cttggcccgc agccactggc tggtaaaatc ctcgccgcgg gtttcgcgct tgcggtaggc
     3661 gtagctgaag ccggtcatgc gatgcgttcc gccgcaagcc gctccgacga tcggctgagc
     3721 aagctcttcc ttgatcacgc gcggaatatc ccaatcctgc ccgtcattgc tcttccactt
     3781 ctcgtcgctc ttcaggtagt gcgtgaaggc gatcagcttg aaggtcaact ccgtcttcgg
     3841 gcggcaggtg tctttctcgt gctggatcaa atccgcgacg gtgaactgct tgccttccac
     3901 cacgagcgga aaggtggtct tcacccgcga ctgggcgagc attgccagga actgtcccgg
     3961 gtggccttgc acgcccgggc cgatccgcgt ctgcagcttg ccattggaga ggtaaaacag
     4021 ctgctggccg ttgcagacgc cgttgtagca gaggtagccg acggcattga cccgcttgtc
     4081 gcccgcaatc aactcgctat ccaccccgta agcgatcatc gcgtgcatcg cgccccacgg
     4141 gctgcgctgg gccacgttct cggggcggaa atagtaataa gccaggcact cgcgaatccg
     4201 cccgcggagc tcttccagct ccgacgtcag ttcgatctct acgatgtcgc gcttggcgct
     4261 ggggacggaa ggcggcgtct ggtcgccatc cccttcggtc ggctcggtct tcggggtact
     4321 gccaaccggg ttgtccttct tcggcgcatc cggctcgtcg tcttcaaacg cgaccaaacg
     4381 caccttggcg ggctgcgcct cgccgctcga gggcaagcgg gacggcttcg cggccgcagg
     4441 cggcgccggg gccggctcgg cactcgagtc ggccggcatt gcgtcgcccg ccgccgggca
     4501 ggtcgcgtcg tggagccact tgctcaccgc gaccagcccg ccaaacaaga ggcaaaagga
     4561 aagaagcgtg cgaatcatca gcgctttcct ggtcggctgc tggtccgggg gggtccgcag
     4621 ccaaagaggc atccgggtag aaacgatctg cgccaacgaa gcggacagcc gccaactg
//
LOCUS       DASRJB010000316         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_940880, whole genome shotgun sequence.
ACCESSION   DASRJB010000316 DASRJB010000000
VERSION     DASRJB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940880"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFB80_08475"
     CDS             complement(<1..486)
                     /locus_tag="VFB80_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN33841.1"
                     /translation="MHAIDLEKSYRKGQHVVPVLKGVDFQVREGELVAVAGQSGCGKS
                     TLLHLLCTLDAPDAGEIHFEGNRIDDLAPRGRDVLRNKYFGMIFQFYHLLPELSALEN
                     VLAPIMIRYGVISYFLHRRQHVERAQALLDLVGLSHRLTHKPRELSGGEMQRTAIARA
                     LI"
     gene            complement(626..2161)
                     /locus_tag="VFB80_08480"
     CDS             complement(626..2161)
                     /locus_tag="VFB80_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN33842.1"
                     /translation="MYKLLLSWRYLRTRYIALASIISVTLGVGTLIVVNSVMAGFARE
                     MHIRLHSILADIVVESHSADGLPNPAWHMEEIRKTVGSDLQGMTAAVHIPAMLNISVR
                     GQWITRPVTLIGIDEATYADVSDFREYLLHPANRQKLSFLLREDGYGDKDHAIPPAGW
                     RYRRMKIITERDLEAQERLLRAAINPGGAGETPLAAEGSTIPPDPTGIPQDPFAGTNT
                     APTFDPAKEQKAGIILGIAIGSVRQRDAAGNVMDYYLCRPGDDVRVTFPSAGTPPKAV
                     SDVCTIVDFYESKMSEYDGGFAFVPLAWLQQQRVMGDAVTTIQLRLVPGADLKLVRDK
                     LRARFPAEEFAYRIQTWRDMQGPLLAAVQMETTLLNILLFLIIAVAGFGILATFFMIV
                     VEKTRDIGILKALGAPSRGVMSIFLSYGFSLGVVGSGVGMIGGLLFVKYINELAKGVE
                     LLTGQEVFDPTVYYFQEIPTVIQPLTVAGVVIGAVAIALLASVLPALRAARLHPVEAL
                     RYE"
     gene            complement(2306..>2669)
                     /locus_tag="VFB80_08485"
     CDS             complement(2306..>2669)
                     /locus_tag="VFB80_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33843.1"
                     /translation="GVHVQGRYEVQVLDSFGLEGKNNECGGIYSIAEPAVNMCLPPLA
                     WQTYDIDFISAKYDADGKKTTNARMTVKHNGVVIHDNLEMKTGNPGYKPEGPEKVGLF
                     LQEHGNPVAFRNIWVVEK"
BASE COUNT          442 a          836 c          892 g          499 t
ORIGIN      
        1 gatgagggcc cgggcgatgg cggtccgctg catctcgccg ccggagagct cgcgcggctt
       61 gtgcgtgagc cggtgcgaga ggccgacgag gtccaagagc gcctgggccc gctcgacgtg
      121 ctggcggcga tggaggaaat agctgatgac gccgtagcgg atcatgatcg gcgcgagcac
      181 gttctccagc gccgaaagtt ccggcagcag gtgatagaac tgaaaaatca tgccgaaata
      241 cttgttccgc agtacgtcac ggccgcgcgg ggcgaggtcg tcgatgcggt tgccttcaaa
      301 gtggatttcg ccggcgtccg gcgcgtcgag cgtgcagagc aggtgcagca gcgtgctctt
      361 gccgcagccg ctctgtccgg cgaccgcgac gagttcgcct tcgcggacct ggaaatcgac
      421 acccttaagc accggcacga cgtgctggcc cttgcggtag ctcttttcga gatcgatggc
      481 gtgcaaaagg gttttgctcg cggttttcgc ggcgtgtgca gggtcgatcc gtggggcggc
      541 ggcctcaggg gactgacgcc ccccgctcgc cccttgctgg cttctgaacc ccgaaccctg
      601 aactctgaac ccttgctcct gcacactact cataacgcag cgcctccacc ggatgcagcc
      661 gtgccgcgcg cagcgcgggc agcacgctgg ccaggagcgc gatcgccacc gcgccgatga
      721 ccacgcccgc gactgtcagc ggctgaatga ccgtgggaat ttcctgaaag taatagacgg
      781 ttggatcgaa cacttcctgg cccgtgagca gctcgacccc cttggccaac tcgttgatgt
      841 acttcacgaa caagaggccg ccgatcatcc cgacgccgct gccgaccacg cccagcgaga
      901 agccgtagct gaggaagatg ctcatcacgc cgcggctggg agcgcccaag gccttgagga
      961 tgccgatgtc gcgggtcttt tcgacgacga tcatgaagaa cgtcgccagg atgccgaagc
     1021 cggcgaccgc gataatcagg aacaggagga tgttgaggag cgtggtttcc atttgcacgg
     1081 ccgccaggag cggtccctgc atgtcgcgcc aggtctggat gcggtaggcg aattcctcgg
     1141 cgggaaagcg ggcccgcagc ttgtcgcgga cgagcttcag gtcggccccc ggcacgaggc
     1201 gaagctggat cgtcgtcacg gcgtctccca tcacccgttg ctgctgcagc caggccagcg
     1261 gcacgaaggc gaagccgccg tcgtactcgc tcatcttgct ttcgtagaaa tcgacgatcg
     1321 tgcagacgtc gctgacggcc ttgggcggcg tgcccgccga ggggaaggtc acgcgcacgt
     1381 cgtcgcccgg gcggcagagg taatagtcca tgacgttgcc cgccgcgtcg cgctggcgga
     1441 cgctgccgat ggcgatgccc aggatgatgc cggccttttg ctccttggcc gggtcaaacg
     1501 tcggcgcagt gttcgtgcct gcgaacgggt cctgcggaat gccggtcgga tcgggcggga
     1561 ttgtgctccc ctcggcggcc agcggcgtct cgccggctcc gccggggttg atggcggccc
     1621 gcagcagcct ttcctgcgcc tccaggtcgc gctcggtgat gattttcatg cggcgatagc
     1681 gccagccggc gggcggaatc gcgtggtcct tgtcgccgta gccgtcctcg cgaaggagaa
     1741 aactcagctt ctggcggttg gccgggtgca gcaggtactc gcgaaaatcg ctcacgtcgg
     1801 cgtaggtcgc ctcgtcgatg ccgatcagcg tgacgggccg cgtgatccac tgcccgcgga
     1861 cggagatgtt gagcatcgcc gggatgtgca ccgcggcggt cattccctgc aggtcgctgc
     1921 cgacggtctt gcggatttcc tccatgtgcc aggctggatt cggcagcccg tcggccgagt
     1981 gggactcgac gacgatgtcg gcgaggatgc tgtgcaggcg gatgtgcatc tcgcgggcga
     2041 atcccgccat gacgctgttg acgacgatca gcgtccctac gcccagcgtg acgctgatga
     2101 tcgaagccag ggcgatatac cgcgtccgca gataccgcca cgaaagcagg agcttgtaca
     2161 taggccatcc atggctgaca aagccgcgac caatggtcgc ggtcttccga cattcgccgc
     2221 ggattgtcgc gagaatcggc cgattgccaa agaccaattt cccgcgaact tctgccgcat
     2281 gcctgacttt ccgtggttgc ctgggctact tctccacgac ccaaatattc cggaacgcga
     2341 ccgggttgcc gtgttcctgg aggaagaggc cgaccttctc ggggccttcg ggcttgtagc
     2401 cgggattgcc cgttttcatt tcgaggttgt cgtgaatcac gacgccgttg tgcttgacgg
     2461 tcatgcgggc gttggtggtc ttcttgccgt cggcgtcgta tttggcggag atgaagtcga
     2521 tgtcgtaggt ctgccaggcg agcggcggca ggcacatgtt cacggccggc tcggcgatcg
     2581 aatagatgcc gccgcactcg ttgttcttcc cctccaggcc aaagctgtcg agcacctgca
     2641 cttcgtaccg cccctgcacg tggacgccg
//
LOCUS       DASRJB010000317         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2911753, whole genome shotgun sequence.
ACCESSION   DASRJB010000317 DASRJB010000000
VERSION     DASRJB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911753"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..799
                     /locus_tag="VFB80_08490"
     CDS             104..799
                     /locus_tag="VFB80_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN33844.1"
                     /translation="MAAPNLETLVAAQFMEEVDRLKQSLALAERDRQLLSYEIHDGIV
                     QDMSAAAMLLEAAGRDATFSSPEVAENFAGGLRLLRESIAEARRIISGLALVEYDASG
                     LDAALDRLVGKFRSELSLPVIYRCEASFPRLPDSAQHLLLRIAQEALFNSWKHAKATR
                     VEVGLKQVGEQLELSICDDGAGFDPKKIPAGHHGVRGMQSRASVLDADLQIQSAAGQG
                     TRVIVRLKAPSRR"
     gene            919..1869
                     /locus_tag="VFB80_08495"
     CDS             919..1869
                     /locus_tag="VFB80_08495"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN33845.1"
                     /translation="MPDLTKTPLPAALLAPEAKLAVAAGICFLEGPAVDKAGNVYFSD
                     IQGNRILKMDAAGIVTTFRADSGRTNGNTFDAQGRLISCEGAENGPGGRRRVVRTELA
                     TGNVEVLTERFEGKRYNSPNDVVVDPAGRIWFTDPLYAPDRSIMEHSHEAVYRIDADG
                     KVTRVITQPAIGRPNGIAVTPDGRTLYVVDSNYISPTANRKIWAFDVAADGSVAKQRQ
                     VYDFGRGRGGDGMRLDSQGNLWVAAGILAPRTANESADVPAGVYVISPRGELIGRIPV
                     AEDVITNLAFGGPDKKMLFVTAGKMLYKIPTAVSGYSLYP"
     gene            complement(2047..2586)
                     /locus_tag="VFB80_08500"
     CDS             complement(2047..2586)
                     /locus_tag="VFB80_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HZN33846.1"
                     /translation="MTRTFSLALILVLFTAAAIAQEQPPTPKPGPEHALLKKLEGTWD
                     AVMKMAEVPAPMKGVATYKMELDGMWLTSEFKMDDPAMKFTGRGLDGFDQNKKKYVGI
                     WVDSMSSAPMVMEGTFDERTKTSTMLGEGPGPDGKPQKYKIVTKHVDDDHQNFEMFMV
                     GADGKESSAFTIAYTRKKK"
     gene            complement(2730..>4231)
                     /locus_tag="VFB80_08505"
     CDS             complement(2730..>4231)
                     /locus_tag="VFB80_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33847.1"
                     /translation="FVPNTAVLPDAYALKKVPLEQFAAAVLPAIPRPSEPQVRDRIRH
                     MVSRLRQLERRFESILFVCSILDWPWIREAYVQQDESAAEDDLVEEAELYQPDERTLI
                     FLLGELPFITGLYERARSELDDEENLAIDGVKELLITARQKYKQDLKRRARKITPHLL
                     RQCLKYTRNLTLFEKRFTPDLYTIVTAAKQTAGDQYALHVAEAAREYPYTQDYGLQPI
                     SLGIDRGRLPDGEIVRLISRLPGPPTIWRSLQLQRRPDRKERDEWRTRWNPFSQCSWP
                     PEDKLIEDFRAHVFDKARAVMGADLARTEPFTTSVMDGIDIRDTLRHWYEEKIYVKIL
                     PPSRGKLDCVVMLFDSPADPRDYPWRTTWYAEHEEESTLAFFATDFGQELVGPGIAQA
                     TYGGALFLFPPVVIPDVWHDAELDFCETLEERLLAAACRYSTCPHIALLSPAPPGAGW
                     RKLAQRFGKKWVHLPLGHFSDATIHQLRIVHVLNGKEVRSYAAEFIRKA"
BASE COUNT          748 a         1365 c         1333 g          785 t
ORIGIN      
        1 aacaagagcg gccggcagga aatgctggag cacctgctga atcggtatat ttaggtggtg
       61 agtggtgagt ggtggtgaat gccgcgctgc aggcagcgta tacatggccg caccaaatct
      121 ggaaacgctc gtcgcggctc aattcatgga agaagtggat cgtttgaagc aatcgctggc
      181 gcttgccgag cgcgaccggc aactgctgag ctacgagatc cacgacggca tcgtgcaaga
      241 tatgtcagcc gcggccatgc tactggaagc tgctggacgc gacgcgactt tttcgtcgcc
      301 ggaggtggcc gagaattttg ccggcgggct gcgcctgctc agggagagca tcgccgaggc
      361 gcggcgaatc atcagcggcc tggcattagt tgagtacgac gcgtcggggc tggacgccgc
      421 cctggaccgg ctggttggca agttccgcag cgaactgagc ttgcccgtaa tttaccgttg
      481 cgaggcgagc tttccgcggc tgcccgactc cgcccagcac ctgttgctga ggattgcgca
      541 ggaggccttg ttcaactcgt ggaagcacgc caaggctacg cgtgtcgagg tgggcctcaa
      601 gcaagtgggc gagcagttgg agttgtcgat ctgcgacgat ggcgcaggtt tcgatcccaa
      661 aaaaatcccc gcgggccacc acggcgtgcg gggcatgcag tcccgagcca gcgtcctcga
      721 tgcggacttg cagattcaga gcgccgccgg ccagggtacg cgcgtgatcg tccgattgaa
      781 agcgccgtcc aggcggtaat atcatccgac tggcctcgcc gcttcgtcac cgttgttgcg
      841 gagaacatca tgctacgccc gctggctgcc gctgtgctgt gttgctgttg ctgttgcccg
      901 acctggagcg ccgctcaaat gcccgatctc acgaaaacgc cgctccccgc cgcgctgctc
      961 gcgcccgagg ccaaactggc tgtcgccgcc gggatttgct ttctggaagg acctgcggtc
     1021 gataaagccg gcaacgtcta tttcagcgac atccagggga accgcatctt gaagatggat
     1081 gccgcgggaa tcgtgaccac cttccgcgcc gacagcggcc ggaccaacgg caacacgttc
     1141 gacgcccagg gccgcttgat cagctgcgaa ggggcggaga acggccccgg cggccggcgg
     1201 cgcgttgtcc gcaccgagct ggccaccggt aacgtcgaag tcttgaccga gcgctttgag
     1261 ggcaagcgct acaacagccc caacgacgtg gttgtcgatc ccgcgggacg gatctggttc
     1321 accgatccgc tctatgcccc cgaccgttcg atcatggagc actcgcacga ggccgtctat
     1381 cggatcgacg ccgacggcaa ggtgacgcgg gtcattacgc agccggcgat cggccggccc
     1441 aacggcatcg ccgtgacgcc cgacggcagg acgctgtacg tcgtggacag caactacatc
     1501 agcccgaccg ccaatcgcaa gatctgggcc ttcgacgtcg ccgccgacgg ctcggtcgcc
     1561 aaacagcggc aggtttacga tttcggccgc ggccggggcg gcgacggcat gcggctggat
     1621 tcacaaggca acttgtgggt tgccgcgggc atcctcgccc cacggaccgc caacgagtcg
     1681 gccgacgtgc cggccggcgt gtatgtgatt tcgccgcgcg gcgagttgat tggccggatt
     1741 ccggtcgccg aagacgtgat caccaacctc gccttcggcg gcccggacaa gaagatgctc
     1801 ttcgtgacgg cggggaagat gctctacaag attcccaccg ccgtcagcgg ctattcgctg
     1861 tatccgtagt ggggcagaca ttcttgtctg cctcatgact cggtcccaag ctccgcttgg
     1921 caccgcaagt tccgaagctc tgcttcgtga gcgggacggg aagccgagct tccggactgc
     1981 gtgcccaagc ggagcttggg cacgaggtgc attcacggca gacaggaatg actgccccac
     2041 gcgcgcctac ttcttcttcc gcgtgtaggc gatcgtaaac gccgagctct ccttgccgtc
     2101 ggcgccgacc ataaacatct cgaagttttg gtggtcgtcg tccacgtgct tggtcacgat
     2161 cttgtacttc tgcggcttgc cgtcggggcc ggggccttct cccagcatcg tcgaagtctt
     2221 cgtcctctcg tcgaaggtgc cttccatcac catcggcgcg gaggacatcg aatcgaccca
     2281 aatgccgaca tacttcttct tgttctggtc gaaaccgtcc agcccgcggc cggtgaactt
     2341 catcgcggga tcatccatct tgaattcgct ggtcagccac atcccatcca gttccatctt
     2401 gtaagtcgcg acccccttca tcggcgcggg cacttcggcc atcttcatca ccgcgtccca
     2461 agtgccctcc agcttcttga gcagcgcgtg ctccggcccc ggcttaggcg tcggcggctg
     2521 ttcctgggcg atcgccgcag ccgtgaacaa gacgagaatc agtgcgagcg agaacgtgcg
     2581 cgtcatgtcg aaaactcctg gaacgggtga gactagaaga ccgacccgta tccaagcgta
     2641 cagcgcgccc tgagtcaact acccccactc aaagccgcga ccaatggtcg cggtttgcgg
     2701 gtagaccgcg accactggtc gcggctttgt tacgctttgc ggataaactc cgccgcgtag
     2761 ctccggacct ccttgccgtt gagcacgtgc acgatccgca gttgatggat ggttgcgtcg
     2821 ctgaaatgtc ccagcggcag gtggacccat ttcttcccga accgctgggc cagctttcgc
     2881 cagcctgcgc ccggcggcgc gggcgagagc agcgcgatgt gcgggcaggt gctgtagcga
     2941 caggccgcgg ccagcagccg ttcttccagc gtctcgcaga aatcgagctc ggcgtcgtgc
     3001 cagacgtcgg gaatcacgac cggcggaaag aggaacaggg ccccgccgta agtcgcctgg
     3061 gcaatgcccg gcccgactaa ctcctggccg aagtcggtcg caaagaacgc cagtgtcgat
     3121 tcctcctcgt gctcggcgta ccaggtcgtc cgccagggat agtcccgcgg atcggccggc
     3181 gaatcgaaca gcatcaccac gcagtcgagc ttgccgcggc tgggtggcag aatcttgacg
     3241 tagatcttct cctcatacca atgccgcagc gtgtcgcgga tgtcgatgcc gtccatcacg
     3301 ctggtcgtga agggctcggt gcgggccaga tcggccccca tcaccgcccg ggccttgtcg
     3361 aagacgtgcg cccgaaaatc ctcgatcagc ttatcctccg gaggccagct gcactgggaa
     3421 aacgggttcc accgcgtccg ccactcgtcg cgctccttgc ggtccggccg ccgctgcagc
     3481 tgcaggctcc gccagatggt cggcggcccc ggcagccggc tgatcagccg cacgatttcg
     3541 ccgtcgggca ggcggccgcg gtcgatgccc agcgaaatcg gctgcaggcc gtagtcttgt
     3601 gtgtacggat actcgcgggc cgcctcggca acgtgcaggg cgtactggtc gccggccgtc
     3661 tgcttggccg cggtgacgat cgtatagagg tccggcgtga accgcttctc aaacagcgtc
     3721 aggttccgcg tgtacttgag gcactgccgg agcaggtgcg gcgtgatctt gcgggcccgc
     3781 cgcttcaagt cctgcttgta tttctgacgg gccgtgatga gcagctcctt cacgccgtcg
     3841 atggccaggt tttcctcgtc atccagttcg gagcgggccc gctcgtaaag gcccgtgatg
     3901 aacggcaact cgcccaacag aaagatcaac gtccgctcgt ccggctgata cagttccgct
     3961 tcttccacca ggtcgtcttc cgccgccgat tcgtcctgct ggacgtacgc ctcgcgaatc
     4021 cagggccagt ccagaatcga acagacaaac aggatcgact caaagcgccg ctccaattgc
     4081 cgcagccggg acaccatgtg ccggatgcga tcgcggactt gcggctcgct tggccgcgga
     4141 atcgccggca gcaccgccgc ggcgaattgc tcgagcggga ccttcttcag ggcatacgcg
     4201 tcaggcagta ctgccgtgtt gggaacgaag c
//
LOCUS       DASRJB010000318         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_373167, whole genome shotgun sequence.
ACCESSION   DASRJB010000318 DASRJB010000000
VERSION     DASRJB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373167"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFB80_08510"
     CDS             <1..305
                     /locus_tag="VFB80_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33848.1"
                     /translation="NLGYTEGGELRRPCNLRRTSFAIVGDAPSDSQQGRRSSNHAGRG
                     QNVLYEDGHVRLVIIIPSPEVLDDPLHNRDGYVAAGLDCNDSCLGRSHDRPMIVVP"
     gene            409..1191
                     /locus_tag="VFB80_08515"
     CDS             409..1191
                     /locus_tag="VFB80_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN33849.1"
                     /translation="MTKSSTTFPVIAGKKTQLTIGGEGPPLLYLHSAGGETDWMPFHA
                     ELAKHFTVYLPAHPGFADSKGLEQIKDIYDLAWHYVDFLAEQKLGPVPVVGFSLGAWT
                     AVELAILRPQLVKKLVLVNAAGVRVEGVKMAELFIDDLNKLRHLLFHDPNDPSVPLAL
                     PKSLEDARIIQWLKAREATARVAWNPYLHNPRLPAHLRRVECPTLVLWGRHDKLLPLA
                     IGEFYAQNIPGARLEIVENCGHMLPFEKPAEFVAKTVGFLRG"
     gene            complement(1195..3918)
                     /gene="purL"
                     /locus_tag="VFB80_08520"
     CDS             complement(1195..3918)
                     /gene="purL"
                     /locus_tag="VFB80_08520"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZN33850.1"
                     /translation="MIHVLPKPGVTDPVAASTLAAIADFQIPVDAVVTLRKYWVGGLT
                     EEQNELLAAKLLANDSIEQVVFGPLKLQRLHLGSPYEFEKITVPIRELNPDQLVALSR
                     ERTLSLTLVELQTIQRHYRELAREPTDIELETIAQTWSEHCSHKTLTGRVTYTDERGT
                     RQFGNLLKETIFAATQEIRKQLGQDDWCVSVFRDNAGVVKFDDEHHVAFKVETHNRPS
                     AIEPYGGANTGVGGVIRDTLGTGLGAKPIANTDVFCFAPPDTPADELPPGVLHPRRIM
                     RGVVSGVRDYGNRMGIPTVNGAIYFDPRYVGNPLVFCGNVGLIPQGKEFKQPRAGDLI
                     VAIGGRTGRDGIHGATFSSAELTHESEKVSGGAVQIGNAITEKMVLDVLLAARDEGLY
                     SAVTDCGAGGFSSAVGEMGAEIGAEVWLDKAPLKYEGLSYTEIWISEAQERMVLAVPE
                     EKWPRLAALCESENVEAVTIGRYVPTGKLELKYRGQTVGQLDMHFLHEGRPKVERQAT
                     YRLPASNPKSPIPNPQSNFGQTLLQLLSSLNIASKEWVIRQYDHEVQGGSVIKPLVGA
                     ANDGPSDAAVLRPVLSSRRGLVIACGMNPRYGDLDPYHMAACAIDEAVRNCVAVGADP
                     RRIAILDNFCWGYTDRPETLGALVRAALACYDVAVAWGTPFISGKDSLNNEFSYSGGG
                     ERRTIQIPHTLLISALGQVEDVRQCVTMDLKTPGNLLYQIGATKNELGGSHWEMISGE
                     REASASRSSTVSQRDNIGKLTLPARQGAIPQVDIQTAKRTFAALHEAIRRGLVRACHD
                     LSEGGLAVALAEMAFAGNCGAAIRLANVPAGDLPAEGRDTALLFAESASRFVAEVAPA
                     NQAAFETILREAKVPCGCIGQVTASGRLQIDALIDLPLTELKEAWQKPLRW"
BASE COUNT          631 a         1355 c         1313 g          732 t
ORIGIN      
        1 cgaacctggg ctacaccgag gggggcgagc tgcgacggcc gtgcaatttg cggcggacga
       61 gctttgccat cgtgggcgac gcccccagcg attcgcagca gggccgccgc tcgtcaaacc
      121 acgccggccg cggccagaac gtgctttacg aagacggcca cgtgcggctg gtaatcatca
      181 ttcccagccc ggaagttctg gacgatccgc tgcacaaccg tgacggctac gtcgccgccg
      241 gcctggactg caacgactcc tgcctcggcc gcagccacga ccggccgatg atcgtcgtgc
      301 cgtagagccc tgcaagtcat ctcggtccca agctccgctt gggaccgcac gcacggaagt
      361 cctctttgcg cctccgcgcc tctgtgcctt tgcgttaaat tacaaggcat gacgaagtca
      421 tcgactacgt ttcccgtcat cgccggcaag aagacccagc tcaccattgg cggcgaaggg
      481 ccgccgctct tgtatctgca ctcggcgggc ggcgagacgg actggatgcc gtttcatgcg
      541 gagctggcca aacacttcac ggtctatctg ccggcgcatc cggggttcgc cgactccaag
      601 ggcctggagc agatcaagga catctacgac ctggcctggc actacgtcga tttcctcgcg
      661 gagcaaaagc tcgggccggt gccggtcgtc gggttctcgc tcggcgcgtg gacggccgtc
      721 gagctggcga ttctgcggcc gcagttggtg aagaagctcg tgctggtcaa cgcggccggc
      781 gtgcgcgtgg aaggagtcaa gatggccgag ctgttcatcg acgatctgaa caagctccgg
      841 catttgctgt ttcacgatcc gaatgacccc agcgtgccgc tggccctccc caagagcctc
      901 gaagacgccc gcatcatcca gtggctcaag gcccgcgaag ccacggcccg cgtcgcctgg
      961 aacccgtacc tccataaccc gcggctgccg gcccatctcc gccgcgtcga atgccccacg
     1021 ctcgtcctgt ggggccgcca cgacaagctc ctgccgctcg cgatcggcga gttttacgcc
     1081 cagaacatcc ctggcgctcg cctggaaatc gtcgagaact gtggccacat gctccccttc
     1141 gagaagccgg ccgagtttgt ggccaagacg gtcgggttcc tgcggggata gccgctacca
     1201 ccgcagcggt ttttgccagg cttctttcag ctcggtcagc ggcaggtcga tgagcgcgtc
     1261 gatttgcagg cggccggaag cggtcacctg gccgatgcag ccgcagggga ctttggcctc
     1321 gcgcaggatc gtttcgaagg cggcctgatt ggccggcgcg acctcggcga caaatcggct
     1381 agcggactcg gcaaacagca gggccgtgtc gcggccttcg gccggcaggt cgcccgcggg
     1441 aacgtttgcc aggcgaatcg cggccccgca attcccggca aacgccatct cggcaagggc
     1501 gacggccagg ccgccttcgc tcaggtcgtg gcaggcgcgg accaggccgc ggcggatcgc
     1561 ctcgtgcagc gcggcgaagg tccgcttggc cgtttggata tcgacctgcg ggatggcgcc
     1621 ttggcgagcg ggaagcgtca gcttcccgat attgtcgcgc tgtgaaaccg tcgatgatcg
     1681 ggaggctgac gcctcccgct cgccggagat catttcccaa tgcgagccgc ccagctcgtt
     1741 cttcgtcgcg ccgatttgat acagcaggtt gcccggcgtc ttcaggtcca tcgtgacgca
     1801 ctgccgcacg tcctcgacct ggcccagagc gctgatcagg agcgtgtgcg gaatctggat
     1861 cgtccgccgc tcaccgcccc ccgaataact gaactcgtta ttgaggctgt ccttgccgct
     1921 gatgaaaggc gtgccccagg cgacggccac gtcgtagcag gccaaggccg cccgcaccag
     1981 cgccccgagc gtctctgggc ggtcggtgta gccccagcaa aagttgtcga ggattgcgat
     2041 ccgccgcgga tctgcgccaa cggccacgca gttccgcaca gcctcgtcga tcgcgcaggc
     2101 cgccatgtga tacggatcga ggtcgccgta ccgcgggttc atgccgcagg cgatcaccag
     2161 cccccgccgc gacgagagca ccggccgcag caccgccgcg tcgctcggcc cgtcgttggc
     2221 cgcccccacc agcggcttga tcacgctgcc accctgcacc tcgtggtcgt attggcgaat
     2281 gacccattcc ttgctggcga tgttgaggga ggagaggagc tggaggaggg tctggccgaa
     2341 attggattgg ggattgggga ttggggattt gggattggac gcaggtagtc gatacgttgc
     2401 ttgccgttcg actttgggtc ggccttcgtg gaggaagtgc atgtcgagct ggccgacggt
     2461 ctggccgcgg tatttgagct ccagcttgcc ggtgggaacg tagcggccga tcgtcaccgc
     2521 ttcgacgttc tcgctttcgc acaaggccgc cagccgcggc catttctcct cgggcacggc
     2581 caggaccatc cgctcctggg cctcgctgat ccagatctcg gtgtaggaga gcccctcgta
     2641 tttgagcggc gctttatcga gccagacttc cgcgccgatc tcggccccca tctcgccgac
     2701 ggcgctggaa aagccccccg cgccgcaatc ggtgacggcg gaatagagcc cttcgtcgcg
     2761 ggcggccagc agcacgtcca gcaccatctt ctcggtgatc gcattgccga tctgcaccgc
     2821 gccgccggag accttttcgc tctcgtgcgt cagctcggcc gaggaaaacg tcgccccgtg
     2881 aattccgtcg cggcccgtcc ggccgccgat ggcgacgatc agatcgcccg ctcgcggctg
     2941 cttgaactcc ttgccctggg gaatcagccc gacgttgccg cagaagacga gcggattgcc
     3001 cacgtaccgc gggtcgaagt agatcgcgcc gttcaccgtg ggaatcccca tgcggttgcc
     3061 gtagtcgcgg acgccgctca ccacgccgcg catgatccgc cgcgggtgca acacgcccgg
     3121 cggcagctcg tccgcaggcg tgtccggtgg agcaaagcag aagacgtcgg tgttggcgat
     3181 cggcttggcc cccaggccgg tgcccagcgt gtcgcggatc acgccgccga cgccggtgtt
     3241 cgcgccgccg tagggctcga tcgcgctggg ccggttgtgc gtctcgacct tgaacgcgac
     3301 gtggtgctcg tcgtcgaact tgaccacgcc cgcgttgtcg cggaagacgc tgacgcacca
     3361 gtcgtcctgg ccgagttgtt ttcggatttc ctgcgtcgcg gcgaagatcg tctctttcag
     3421 caagttgccg aactgccgcg tgccgcgctc gtcggtgtaa gtcacgcggc cggtcagcgt
     3481 cttgtggctg cagtgttcgc tccaggtctg ggcgatcgtc tccagctcga tgtcggtcgg
     3541 ctcgcgcgcg agctcgcggt agtgccgctg aatcgtctgc agctcgacca gcgtcaggct
     3601 cagcgtccgc tcgcggctca gggccaccag ctggtccgga ttcagctcgc ggatggggac
     3661 cgtgatcttc tcgaattcgt acggcgaacc gagatgcagc cgctggagct tcagtggtcc
     3721 gaagacgacc tgctcgatcg agtcgttggc gagcagcttc gccgccagca gttcgttttg
     3781 ctcttcggtc agtccgccga cccagtattt tcgcagcgtc acgacggcat cgacgggaat
     3841 ctggaagtcg gcaatcgccg ccagcgtgct cgccgcgacc gggtcggtca cgcccggctt
     3901 gggcaggacg tggatcaagg cgagcgggag gcgtaagcct cccgatgatt gcgcatctcg
     3961 ggtggccgag ttatcgggag gctgacgcct cccgctcgcc ggcagctcgc tgaccgccaa
     4021 ctcgcccgtc c
//
LOCUS       DASRJB010000319         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_90490, whole genome shotgun sequence.
ACCESSION   DASRJB010000319 DASRJB010000000
VERSION     DASRJB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90490"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFB80_08525"
     CDS             <1..520
                     /locus_tag="VFB80_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN33851.1"
                     /translation="YGNPWSLDSYTGYQPRQRTPQEQILISAAGPGIQFVLAGLVIAA
                     VYASGGTVRLLLHNYMPYVDAALGPNLAHNRHLDLLVWDLLQINIFWAIMNLLPVLPL
                     DGGQIALQVFMQHDPWQGTIRALWLSVITGGIVAVLTLLAGETYMMFLFASLAVSSYM
                     TLLQAGGGRRPW"
     gene            complement(1131..2219)
                     /locus_tag="VFB80_08530"
     CDS             complement(1131..2219)
                     /locus_tag="VFB80_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZN33852.1"
                     /translation="MSAAPISVADLQKALADFKDPETGLAAAGQKQIRDVEVSGNGAA
                     LTLALSTHSAAIREETKQRLIDFLRARLPGLADVKVELAVHERPPQKLGQVGLTSKSV
                     IAVGSGKGGVGKSTIAASLALGLLKAGSKVGLLDTDVYGPSIPHLLGLTGAPQPTVVD
                     GKVPPVMAGPMPVMSMGFLLPPGKAVIWRGPMLHSYVTQFLRDVNWGDLDYLIIDMPP
                     GTGDIALSLSQVLPLTGAVIVCTPQPVALIDAERAISMFRTVNIPILGMVENMSGFIC
                     EGCGKRYDIFGSGGARQKAEELNVPFLGEVPLNISIRTSGDAGRTAANFSDPIVAPYL
                     EKMVAALVRNLATTAAAKPPMMQLPVLK"
     gene            complement(2247..2690)
                     /locus_tag="VFB80_08535"
     CDS             complement(2247..2690)
                     /locus_tag="VFB80_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33853.1"
                     /translation="MSAVRLIVCEGQGRWAAALRASLGGDASLIAETRSLSQCEQALS
                     AAPASVVAVEAAADNAQAVIAFFLRAARFPQARLAALVAAEFADAEPLLREAGALDVL
                     STVADAPRLAQLALRQKKLQPEEPQSLEEFIGQRLPWPAHASDPA"
     gene            2862..>3713
                     /locus_tag="VFB80_08540"
     CDS             2862..>3713
                     /locus_tag="VFB80_08540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33854.1"
                     /translation="MPAASSRAGADPEKSVHQLLEDWQARAARGERLQPHEMLTALAE
                     ATSAASPDLPATVELPGNQSATVSIRPPAEGASPSGAFERLSSRWKALSGGGNGEPAA
                     DGLADYELLREVARGGMGVVYEVRQKSLNRIVALKMIRAGELADAEDVARFYSEAEAA
                     ANLRHPNIVAVYEVGRAGPAGEHHYFTMDFVAGPSLLDLVRKQPLDGRTAARYVAKVA
                     RAIQFAHDRGVLHRDLKPANILLSESDEPLVTDFGLAKRIGADSGVTIAGTVLGTPSY
                     MPPEQAAG"
BASE COUNT          631 a         1249 c         1239 g          594 t
ORIGIN      
        1 ctacggcaac ccctggtcgc tcgattcgta cacgggctat cagccgcggc agcgcacgcc
       61 gcaggaacag attctgattt cggcggcggg gccggggatt cagttcgtcc tggccggcct
      121 ggttatcgcg gcggtgtatg ccagcggcgg gaccgtgcgg ttgctgctgc acaactacat
      181 gccctacgtg gacgccgcgc tgggccccaa cctagcgcac aaccggcatc tggacttgct
      241 ggtgtgggac ctgctccaga tcaacatctt ctgggcgatc atgaacctgc tgccggtgct
      301 gccgctcgac ggcgggcaga tcgcgctgca agtcttcatg cagcacgatc cgtggcaggg
      361 cacgatccgg gcgctgtggc tgtcggtgat caccggcggc atcgtcgcgg tgctcacgct
      421 gctcgcgggc gagacctaca tgatgttcct gttcgcgtcg ctcgcggtct cgagctacat
      481 gacgctgctg caagccggcg gcggacgcag gccgtggtga tgctgcgcgc atgcgcgtgc
      541 gctttgtcgt tagcgcgatg cctggtgcgc gaagtcgcgc gcaatcgcgc atttcctctt
      601 gacgattccg gaagcaaacg gaagatatag ttcaacttgt gctagcgatg agctgctgtg
      661 acagcatcgc tggtgccagg aatgccaagg agttgaaagt ggatcgagcg gagcgcgata
      721 tccgaaacgc ggattctcgc gcgagcgctc gggccgcttt gtggcacgcg gtggtgagtt
      781 ccggcgctgc aaggtgcagc gcgacgatcc tcaccgccta acctttatcg gaggaagcac
      841 agtggccaaa aagaaagctg ccaagaagaa gaagaccgcg aagaagaaga agaagtagca
      901 ggccgcggtc gccggacttc ccggcagcca ggagggctgg gccgcggcag ccgtcgcttg
      961 gcgagcagca cgacgctgcc cgcccttggg acgccggccg cccggttcgc agctttggaa
     1021 aacaacgaga aagagccgtt aaaacgcagg ttttagcggc tctagtcttt tcttggcaga
     1081 ggtcagagct tagaggttag tctcctgaac tctgaactct aacctcaacc tcacttcagc
     1141 accggcagct gcatcatcgg cggcttggcg gcggcggtcg tcgcgagatt gcggacgagc
     1201 gccgccacca tcttctccaa atacggcgcg acgatcggat cgctgaagtt cgcggccgta
     1261 cggccggcgt cgcccgacgt gcggatgctg atgttgagcg gcacttcgcc caggaagggc
     1321 acgttcagct cctccgcctt ctgccgcgcg ccgccgctgc caaaaatgtc gtaccgcttg
     1381 ccgcaaccct cgcagatgaa cccgctcatg ttctcgacca tgccgaggat cgggatgttc
     1441 acggtgcgaa acatcgagat cgcccgctcg gcgtcgatca gcgccacggg ctgcggcgtg
     1501 cagacgatca ccgcgccggt cagcggcagc acctgcgaca gcgagagcgc gatgtcgccc
     1561 gtgcccggcg gcatgtcgat gatcagatag tcgaggtcgc cccagttcac gtcgcggagg
     1621 aactgcgtca cgtacgagtg cagcatcggt ccgcgccaga tcaccgcctt gcccggcggc
     1681 agcagaaagc ccatcgacat caccggcatc ggcccggcca tcaccggcgg caccttgcca
     1741 tcgacgaccg tcggctgcgg cgcgcccgtc aggccgagca ggtggggaat gctggggcca
     1801 tagacgtcgg tatcgagcag gcccaccttg ctgcccgctt tcagcaggcc cagggccagc
     1861 gacgcggcga tcgtgctttt cccgacgccc cctttgcccg agccgacggc gatcacgctt
     1921 ttggacgtca ggccgacctg accgagcttc tgcggcggcc gctcatgaac cgcgagttcc
     1981 actttcacat cggccaggcc cggcagccgc gcccggagaa agtcgatcag ccgctgcttc
     2041 gtctcctcgc ggatcgctgc cgagtgcgtc gagagcgcca gcgtcagcgc cgccccattg
     2101 ccgctaacct cgacgtcgcg gatctgcttc tggccggcgg ccgccaggcc ggtctccggg
     2161 tccttgaaat cggccagggc cttctgcaga tcggctacgg aaatgggagc ggcggacatg
     2221 tggtctttca gggaaaggtt gacgaatcag gcgggatcgc tggcgtgtgc cggccatggc
     2281 agccgctggc cgatgaattc ttccagcgac tgcggctcct ctggctggag ttttttctgc
     2341 cgcagggcga gctgtgccag tcgcggcgcg tcggcaaccg tcgagagcac atccagcgca
     2401 ccggcttcgc gcaacagcgg ctcggcgtca gcgaactcgg ccgcgacgag cgccgcgagg
     2461 cgggcctgcg gaaaacgggc cgcgcgaagg aagaaagcga tcaccgcttg ggcattgtcc
     2521 gcggcggcct cgaccgcgac taccgaagcc ggtgctgcgg acagcgcctg ctcgcactgc
     2581 gacaggctcc gcgtttcggc gatcagcgac gcgtcaccgc ccagcgacgc gcgcagcgcc
     2641 gccgcccacc ggccctgccc ttcgcaaacg atcaatcgca cggctgacat cttatctcca
     2701 gtgccagaca tgcatcctag gtggcccaac ccgggcgggc aaccggcggc acgagcgtcc
     2761 taagtccttg ccgcgctgcg ggttactcac gcacccagcg gccgcgaaag ctggccattg
     2821 ccgatcgccc tataatcaag gaccgcttcg cgagcttacg catgcccgcc gcttcatctc
     2881 gggccggggc cgatccagag aagtccgtgc accaattgct ggaggattgg caggctcgcg
     2941 ccgcgcgcgg cgagcggctg cagccgcacg aaatgctcac cgcgctggcc gaggcgacga
     3001 gcgccgccag ccccgacctg ccggctaccg tcgaattgcc cggcaaccag tcggcgacgg
     3061 tctccatccg cccgcccgcg gagggcgcct cgccgagcgg cgcgttcgag cggctgtcgt
     3121 cgcggtggaa ggcgctctcg ggcggcggca acggcgaacc cgccgcggac ggcctggccg
     3181 attacgaact gctccgcgaa gtagcccgcg gcggcatggg cgtggtctat gaggtgcggc
     3241 agaagagcct caaccggatt gtcgccctga agatgatccg cgccggcgaa ctggccgacg
     3301 ccgaggacgt ggcgcggttc tattccgagg ccgaagcggc ggcgaacctg cggcatccga
     3361 acatcgtcgc ggtttacgaa gtcggccgcg ccggaccggc cggcgagcac cactacttca
     3421 cgatggactt cgtcgcgggc ccgagcctgc tcgacctggt ccgcaagcag ccgctggatg
     3481 gccgcaccgc cgcccgctac gtggccaagg tcgcgcgggc gatccagttc gcccacgacc
     3541 gcggcgtgct gcaccgcgac ctcaagccgg cgaacatcct gctgagcgag agcgacgagc
     3601 cgctggtgac cgacttcggc ctggccaagc ggatcggcgc cgattcgggc gtgacgatcg
     3661 ccggaacggt gctggggacg ccgagctaca tgccgcccga gcaggccgcg ggc
//
LOCUS       DASRJB010000320         8567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3755972, whole genome shotgun sequence.
ACCESSION   DASRJB010000320 DASRJB010000000
VERSION     DASRJB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755972"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..3543
                     /locus_tag="VFB80_08545"
     CDS             148..3543
                     /locus_tag="VFB80_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN33855.1"
                     /translation="MSKSTLDGGVYVEEGIEISEAFKLPTPETPERKIVKILAQIGEN
                     LDQLEKLAKTTDAESTEQAVRTYRSQLIELSRKAGQNEKEDKLVAKGVELLIQIKAEL
                     SVPAREGNVVKAIGEFKKHAGPPGKPKAAEFELEKSRIDGLANPIVRCSDYWELEREV
                     TKADLYFQEQANPGSTWQTRMDFAVEDAMPHNLPKEGAQEGAKIGAKEGFFSVVPLGT
                     LIGAGLGAAIGAVAGKIKESREKPPLDAGEWQQMGKSFAASMDKKIADMTFSVPQEAV
                     AEFTGAARQAIQEIREEHFAADRLPDTRQKMGKALMVANTAALSQLSTLKRDYPADAT
                     PQVFVTGGKMTVAKQAPKLESLVLQGGGGKGVGFPAMLEEMNKSGMLENVDLLVGTSI
                     GALNAACLACGGLSDEREILNLEIMKQAYDSKNSKKKYPGVTFGKDAWPSCAGQMAKM
                     DELTSKSVTEKLKSHTEEQLTDDLVAKLQALDDDALERMGLKGADDAAIQKQVRALAK
                     KVKHQDFGASDRTTQMITFQDLAILHQLDPKNFKELTITGWEGTGKEGQSVYFNAKDF
                     GDMPVALAARISMGLPIFSPVYWKGRGPFYDGGLGSNAPTEAAPGLDKFYAEHDPADA
                     EEILKGDDIPLEVQQAMQKTMLMTFDNEGAGNRNLYGDGRLTAAPSLGEKILVEKKYQ
                     IPDAINPDYGGALKRDASKVHNSGVNTFQVFHGKEGTMSLGVLGGNAEEIEYAENMAR
                     LKGLEQIDQRADQAVAVSCKSADEALSTFSEGEMKKFVAAGLPASADPMVQELFRKCQ
                     AYLDLDVASKQAKSTGDSTTFVNELVVSQLTATCQAQARQLQNAYQVGVKGNPVPDAL
                     KQEVAASKSAIGGLPTYLQAMVKSAVLVPLQQKVRALGQDPANEPPPSFVWKRDFSAK
                     TFDETIRAAAKAGVFPKTKDAENVTDAFQSYLRADQNQLDKKKSKDRTKAAREALNKL
                     DELLQNIKKMAESAAYAGNATMQKYLGWLTDRARGQEAPLRSIVKGAAADFPKTSFKP
                     WDEKDWQKNKKAAISAGALEDYGATGLEDAMEAAEKAVQAARQATAPDVRTATGKNAW
                     KAWDHVIRAAKGVQSHTENDNFTAYLEQCVTQATAEREKYPAP"
     gene            3607..3927
                     /locus_tag="VFB80_08550"
     CDS             3607..3927
                     /locus_tag="VFB80_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33856.1"
                     /translation="MSSLNPYAAPATLTPQGGERPATALGVVRAFLIVAIAAGACAIA
                     GAILGSLIGWLLPDYYHAVFGNPTLNTVQVGIGLGLTQGLGAGFVVGVMVVLAVAISI
                     RRRA"
     gene            complement(4016..4483)
                     /locus_tag="VFB80_08555"
     CDS             complement(4016..4483)
                     /locus_tag="VFB80_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33857.1"
                     /translation="MSSAVLTERTTRGAFATGPAPTAGPIAAPAANVCVVPRCEISFE
                     KTKSGCKIHCRCPDQVSAATLQSLCQALCDGTCSCCCTWNGIQCCNISLCCGHCKCEN
                     TKDGCCITCTSGDSKCCEMIQACCECCETCCNSGCCCYICFGNTPVCCGTCAA"
     gene            4879..5100
                     /locus_tag="VFB80_08560"
     CDS             4879..5100
                     /locus_tag="VFB80_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33858.1"
                     /translation="MFTNRIKNYPSDEEAIVAPALPAGFAVCQLPQVATNPLAIQLIA
                     QQQAFEQALIAAKEEALRIVLSRLQTSVN"
     gene            complement(5103..5561)
                     /locus_tag="VFB80_08565"
     CDS             complement(5103..5561)
                     /locus_tag="VFB80_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZN33859.1"
                     /translation="MMAPRSLLALAISLLLAPTARADAEADAAVKAIEQVLRVQAEAW
                     NRGDIAAFMEHYWKSDELTFSSGGQTTRGWQATKDSYLKRYPTRELMGKLAFSQLEIK
                     PLGADAALVLGRWQLDREMPVGGNFSLVLRRMDGRWLIIHDHTSRTPANP"
     gene            complement(5558..6586)
                     /locus_tag="VFB80_08570"
     CDS             complement(5558..6586)
                     /locus_tag="VFB80_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN33860.1"
                     /translation="MSPSSKDRSPRRGPGISWSSVLFVVFVLAALAALAAWNNGSAWQ
                     RPPAASKLSLAQIPFDGERAFGHLKAICAIGPRVSATDGMLRQQTYLKEHFSKLGGKV
                     TMQEFPARHPETGNNVQLANLIVEWHPERTKRILLCAHYDTRPFPDQDPANPRGLFVG
                     ANDGASGVAVLCELAQHMPQLKSNYGVDFVLFDGEELIFDADRDPYFLGSEHFAREYK
                     LKPPKHQYAYGVLLDMVGDTNLQLYREIGGMKEVPTRELTNDIWSVARSLGVREFIFQ
                     NKHEVRDDHLALKEIAGIPAIDIIDFEYPTSRINYWHTTKDVPENCSALSLAKVGWVL
                     KTWLERVK"
     gene            complement(6670..>8567)
                     /locus_tag="VFB80_08575"
     CDS             complement(6670..>8567)
                     /locus_tag="VFB80_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33861.1"
                     /translation="LAAAATEKRGPPTTVAGDGKWLEQLLISGLSGVIHFQAVGLSLG
                     PDGCLYVACGTSDNRARSWDGSQATVLRSGAVFRARPDGSQLREFARGFCQPRGCLTF
                     DPLGNPFLADVDWTVGKGGGRLIHVLEGGNYGWRHGGGAAAGGELAGTLPGMLRGDSA
                     ASTGVSFVNFANGLLISANGDRLRAFATQPDDATHRAISQFALLESDDPLFRPCQAIT
                     GPDGALYVVDWHSTGDFSSGTGGSKQGRIFRLSWSGWKDMPAVSLAPRDRWAKLAADS
                     DEQLLAHLEASDPELRRRAGQEIAARAHKDPAQAAKFRPRLAALAADESKSISVRASA
                     IPAAGQLLDRPLREALGELLTHKDADLVRLAAEALGNSPPAQEEELAAVAESFKGALS
                     GTADPAKLRALALGLGKLAAAAKSVDLAEWNFEGTSVSHGPKMSRYVFDGHARSLELV
                     KGAAKELMLGNLDVALNLPEAEPAERQRLKEFVTLTAEAMRTRELAEFLDALLLQEEN
                     WFVRLPAPLEARLLACYQNILVDPPINADAVATWLDKNPGGPAEVEVAALETVSLVGT
                     TKEGYVEKLADRLLAKPAEAALVGRSFAAGRLDRKLLPRIKEALTRHAASDSTGDAAA
                     VLKQLEK"
BASE COUNT         1648 a         2728 c         2811 g         1380 t
ORIGIN      
        1 agagtggaac tgaacagaag gaagcaaaga tagcaaaggc gggagggggg caccccgaag
       61 ccgattccct tctgcattct gctttgcttc cttggctacc ttctgttcct tcatccatat
      121 tccaacgacc gcggaggaga gataggcatg tcaaagagca ctttggacgg cggcgtttat
      181 gttgaagaag gaatcgaaat ctccgaggcc ttcaagctgc cgacgccgga gacgcccgag
      241 cgaaagattg tcaagatcct cgcgcagatc ggcgaaaacc tcgaccagct ggagaagctc
      301 gccaaaacga cggacgccga atcgaccgag caggccgtgc ggacctaccg cagccagctg
      361 atcgagctgt ccaggaaagc cggccagaac gagaaggagg acaagctggt cgccaagggg
      421 gttgagctgc tcattcagat caaggccgag ctgagcgtgc cggcgcggga aggcaacgtt
      481 gtcaaagcga tcggcgaatt caagaagcac gccggcccgc ccggcaagcc gaaggccgcc
      541 gagttcgaat tggaaaagag ccggatcgac ggcctcgcca acccgatcgt gcgttgcagc
      601 gactactggg agctggagcg cgaggtcacc aaagccgacc tctactttca ggagcaggcc
      661 aatcccggct ctacctggca aaccaggatg gactttgcgg tagaagacgc catgccgcac
      721 aacctgccca aggagggcgc gcaggaggga gcgaagatcg gtgcgaagga aggtttcttc
      781 tcggtagtgc cgctggggac gctgatcggt gccggcctgg gcgcggcgat cggcgcggtt
      841 gcgggcaaga tcaaggagag ccgcgagaag ccgccgctgg acgcgggcga atggcagcag
      901 atgggcaagt cgttcgcggc ctcgatggac aagaagatcg ccgacatgac gttttcagtg
      961 ccgcaggagg cggtggccga attcacgggc gcggccaggc aggcgatcca ggaaatccgc
     1021 gaggagcact ttgcagcgga tcgcctgccc gacacgcggc aaaagatggg caaggcgctg
     1081 atggtcgcca acacggcggc cctgtcgcag ctgagcacgc tcaagcgcga ttatccggcc
     1141 gacgcgacgc cgcaggtatt cgtcaccggc ggcaagatga ccgtcgccaa gcaggcgccc
     1201 aagctggaga gcctcgtgct ccagggaggc ggcggcaagg gggtcggttt cccggcgatg
     1261 cttgaggaaa tgaacaagtc gggaatgctg gagaacgttg atctcctcgt gggaacgtcg
     1321 atcggggcgc tcaacgccgc gtgcctggcc tgcggcggcc tctccgacga gcgggagatc
     1381 ctcaacctgg aaatcatgaa gcaggcgtac gactcgaaga acagcaagaa gaagtatccc
     1441 ggcgtgacgt ttggcaaaga cgcctggccc tcgtgcgccg gccagatggc caagatggac
     1501 gagctgacca gcaagtcggt caccgagaag ctcaaatcgc acaccgaaga gcagttgacc
     1561 gacgacctgg tcgccaaact ccaggccctc gacgacgacg ccctggagcg aatgggcctg
     1621 aagggggccg acgacgcggc aatccagaag caggtgcggg ccctggccaa gaaggtcaag
     1681 caccaggatt tcggcgccag cgaccgcacc acccagatga tcaccttcca ggacctggcg
     1741 atcctgcacc agctcgatcc caagaacttc aaggagctga cgatcacggg ctgggaaggg
     1801 acgggcaagg agggccagag cgtctatttc aacgccaagg actttggcga catgccggtg
     1861 gccctggccg cccgcatttc gatgggcttg ccgattttct cgcccgtcta ttggaagggc
     1921 cgcgggccgt tctacgacgg cggcctcggg tccaacgccc cgacggaggc cgcgccgggc
     1981 ctggacaagt tctatgccga gcacgacccg gccgacgccg aggaaatcct caagggggac
     2041 gacatcccgc tggaagtgca gcaggcgatg cagaagacga tgctgatgac gttcgacaac
     2101 gaaggcgcgg gcaacaggaa tctgtacggc gacggccggc tgaccgccgc gcccagcctg
     2161 ggcgagaaga tcctggtcga gaagaagtac cagattcccg acgcgatcaa cccggattac
     2221 ggcggagcgc tgaagcgcga cgcgagcaag gtccacaaca gcggcgtcaa cacgttccag
     2281 gtgttccacg gcaaggaagg cacgatgtcg ctgggcgttc tcggcggcaa cgcggaggag
     2341 atcgaatacg ccgagaacat ggcccgcctg aaggggctgg agcagatcga ccagcgggcc
     2401 gaccaggccg tggccgtctc gtgcaagagc gccgacgagg ccctctcgac cttcagcgaa
     2461 ggcgaaatga agaagttcgt cgccgctggc ctgccggcga gcgccgaccc catggtccag
     2521 gagctgttcc gcaagtgcca ggcgtatctc gacctcgacg ttgccagcaa gcaggccaag
     2581 agcacgggcg actcgacgac gttcgtcaat gaactggtcg tttcgcagct gacggcgacc
     2641 tgccaggctc aggccaggca attgcaaaat gcctaccagg tcggtgtgaa gggcaatcct
     2701 gtgccggacg cgctaaagca ggaagtcgcc gccagcaagt cggccattgg cggcctgccc
     2761 acgtatctgc aggcgatggt gaagtccgcg gtgctcgtgc cgctgcaaca gaaggtgcgg
     2821 gccttgggcc aggaccctgc gaacgagccg ccgccctcgt ttgtctggaa gcgcgacttt
     2881 tcggcaaaaa ccttcgacga gacgatccgc gcggctgcca aggcgggcgt cttcccgaag
     2941 accaaggacg ccgagaacgt cacggacgcg ttccagtcgt acctcagagc ggaccagaat
     3001 cagctcgaca agaagaagtc caaggaccgc accaaggcgg cccgcgaagc gctgaacaag
     3061 ctcgacgagc tgctgcagaa catcaagaag atggccgagt ccgcggcata cgccggcaat
     3121 gccacgatgc agaaatacct gggctggctg accgaccggg cccgcgggca ggaagcgccg
     3181 ctccgctcga tcgtcaaagg cgcggccgcc gacttcccca agacttcgtt caagccctgg
     3241 gacgagaaag actggcagaa gaacaagaag gcggcgatca gcgccggcgc gctggaagac
     3301 tacggcgcga ccggcttgga agatgcgatg gaggccgccg aaaaggctgt ccaggccgcg
     3361 cgccaagcga ccgcgccgga cgtgcggact gccaccggca agaacgcctg gaaggcctgg
     3421 gaccatgtca ttcgcgccgc caagggcgtc cagtcccaca cggagaacga taacttcacg
     3481 gcttacctgg agcagtgcgt tactcaggcg accgccgaac gggagaagta cccggcgccg
     3541 tagtcgcaag caataatgga agacggaccg ccaatccgct ctcagttaca tctgggcacg
     3601 acgacaatga gctcgctcaa tccgtacgcc gcacctgcta cgctgacgcc gcagggcggc
     3661 gagcgacccg ccactgcact cggcgtggtg cgcgccttcc tgatcgtcgc gatagccgcc
     3721 ggtgcctgtg cgatcgccgg agcaatcctc ggcagcctga tcggctggct gctgcccgat
     3781 tactaccacg cggtgttcgg caacccgact ttgaacacgg tccaggtggg gatcggcctg
     3841 ggcctgacgc aaggcctggg cgcgggcttt gttgtcggcg tgatggtggt gctggccgtc
     3901 gccatctcca tccgccgccg cgcgtaagta gcgcccccag ggtcagctgg cgcaaaaaag
     3961 taggacggac tggcaagtcc gtcctacgcg tgacgttcgc gatcttcggg tggcactacg
     4021 ccgcgcaggt gccgcagcag acgggcgtgt tgccgaagca gatgtagcag cagcagccgc
     4081 tgttgcagca ggtctcgcag cattcgcagc aggcctggat catctcgcag cacttgctgt
     4141 cgccactggt gcaggtgatg cagcagccgt ccttggtgtt ctcgcacttg cagtggccgc
     4201 agcagagcga gatgttgcag cactggatgc cattccaggt gcagcagcag gagcaggtgc
     4261 cgtcgcacag cgcctggcag aggctctgca gcgtggcggc ggaaacctga tccgggcagc
     4321 ggcagtggat cttgcagccg ctcttcgttt tttcaaacga aatctcgcac cgcggcacca
     4381 cgcacacgtt tgccgccggc gccgcaatcg gcccggcggt cggcgccggt ccggtggcaa
     4441 aggcgccccg cgtcgttcgt tcggtcaaga cagcgctaga catcaagtgc actccttttc
     4501 gtgggggccg ctccgctcgg ccaacgcggc aggcagattc aggcccgaaa tgtcacaccc
     4561 gcgctacctc tgggttccca gtcagccggc gtcgccaaag aaaagtcgcg cctccctggg
     4621 cggggtcggc ctcgtgagta gccaatgtcg tatcacgcag ggccaaaaac tgtcaaccaa
     4681 actttgcggt ttgtgatgcc tgtgccgcca gatttttcca tcttccgcga gggatgcggc
     4741 gtttcgtccg gagccgtgcc agcggcgtcc gatagaatga aagatgatga acttagtcac
     4801 atttggccgc ggcatgcgct gtgcatattg agggagttag ggagaaaaca ccgcaaattt
     4861 cgaggattgg ggccggtcat gttcaccaac cgtatcaaga attacccgtc tgacgaagaa
     4921 gcgatcgtcg ccccagcgct tccggcaggc tttgcggtct gtcagctccc gcaagttgcc
     4981 acgaatcccc tggcgatcca gctcatcgcc cagcagcagg ccttcgagca ggcgctgatc
     5041 gccgcgaagg aagaagccct gcgaatcgtg ctcagccgct tgcagacctc cgtgaactag
     5101 cctcacggat tggccggcgt acgcgacgtg tggtcgtgaa tgatgagcca gcggccatcc
     5161 atccgccgca gcacgagcga gaagtttccg ccgacgggca tttcgcgatc cagctgccag
     5221 cggccgagca ccagcgcggc gtccgcgccc agcggcttga tctcgagctg cgagaaagcg
     5281 agcttgccca tcagctcgcg ggtgggatag cgcttgaggt agctgtcctt cgtggcttgc
     5341 cagccgcggg tcgtctggcc gccggagctg aacgtcagct cgtccgattt ccagtagtgc
     5401 tccatgaagg ccgcgatgtc gccgcggttc caggcctcgg cctgaacgcg cagtacctgc
     5461 tcgatggcct tgacggctgc gtcggcctcg gcatcggccc gagcggtcgg cgcgagcaga
     5521 agggagatcg ccaatgccaa cagcgatcgt ggcgccatca tttgacgcgc tccagccagg
     5581 tcttcaggac ccagccgact ttggccagcg acagggccga gcagttctcg ggcacgtcct
     5641 tggtcgtgtg ccagtagttg attcgcgacg tgggatactc gaagtcgatg atgtcgatcg
     5701 cggggatgcc ggcgatctcc ttgagggcca gatggtcgtc gcggacttcg tgcttgttct
     5761 ggaagatgaa ctcgcgaacg cccaggctgc gggcgacgga ccagatgtcg ttggtcagct
     5821 cccgtgttgg cacttccttc atgccgccga tctcacgata gagctgcagg ttggtgtcgc
     5881 cgaccatgtc gagcaagaca ccgtaggcgt actggtgctt cggcggcttg agcttgtact
     5941 cgcgggcaaa gtgctcgctg cccaggaagt acgggtcgcg gtcggcgtcg aagatcaatt
     6001 cttcgccgtc gaacaggacg aagtcgacgc cgtagttgct cttgagctgc ggcatgtgct
     6061 gggccagctc gcagagcacg gccacgccgc tagcgccgtc gttggcgccg acaaacaggc
     6121 cccgcggatt ggccgggtcc tgatcgggaa agggccgcgt gtcgtagtgg gcgcagagca
     6181 gaatccgctt cgtccgctcc gggtgccatt ccacgatcag gttggcgagc tgcacgttgt
     6241 tgcccgtctc cggatggcgg gcgggaaact cctgcatcgt caccttgccg ccgagcttgc
     6301 tgaaatgctc cttcaaatag gtctgctgcc gcagcattcc atcggtggca gaaacgcgcg
     6361 gcccgatggc gcagatcgcc ttgaggtggc cgaaggcccg ctcgccgtcg aagggaatct
     6421 gggccagcga cagcttcgat gccgccggcg gccgctgcca ggccgagccg ttgttccagg
     6481 ccgctaatgc cgccaaggcc gccagcacga acacgacgaa cagcacgctc gaccaggaaa
     6541 tgccgggccc gcgtcgcggc gagcgatctt tgctggaagg ggacaactgc cggctcctat
     6601 cacggaacgt tcagcatagc cgccaaagcg cggttttccc atgcgagttg agggcgcggc
     6661 cagcggccgc tacttttcaa gctgcttcaa gaccgcagcc gcgtcgcccg tgctgtcgct
     6721 cgcggcgtgc ctggtgaggg cttccttgat tcgcggcagc agcttgcggt cgagccgccc
     6781 ggcggcgaag ctgcggccga cgagcgcggc ttcggcgggt ttggccagca gccggtcggc
     6841 cagtttttcg acgtagccct ctttcgtcgt accgacgagc gacaccgttt cgagcgcggc
     6901 gacttcgacc tccgcggggc cgccgggatt cttgtccagc caggtggcga ccgcgtcggc
     6961 gttgatcggc ggatcgacca ggatgttctg gtagcaggcc agcagccggg cttcgagcgg
     7021 cgctggcagg cggacgaacc aattctcttc ctgcagtagc agggcatcga ggaattccgc
     7081 cagctcgcgg gtccgcatcg cctcggcggt gagcgtcacg aactccttga gccgctgccg
     7141 ctccgccggc tccgcctcgg gcaggttgag ggccacgtcg aggttgccca gcatcagctc
     7201 cttggccgcg cccttaacca gctccagcga ccgcgcgtgg ccgtcgaaca catagcggct
     7261 cattttcggg ccgtgggaga ccgacgtgcc ttcgaaattc cactccgcca gatcgacgct
     7321 cttggccgct gccgcgagct tgcccaggcc cagggccagc gcccgcaatt tggccggatc
     7381 ggccgttccg ctcagcgctc ccttgaagct ctcggcgacc gcggccagct cttcttcctg
     7441 tgctggcggc gaattgccga gcgcctcggc tgccagccgc acgagatcgg catccttgtg
     7501 cgtcagcaat tcgcctagcg cttcccggag cggcctatcg agcagttgac cggcagccgg
     7561 gatcgcgctg gccctgaccg aaatcgattt cgactcgtcg gccgccagag cggccagtcg
     7621 cggacggaat ttggcggcct gcgccggatc cttgtgagcg cgggcggcga tctcttgccc
     7681 ggcgcggcgg cgcagctccg gatcgctcgc ttccaagtgc gcaagaagct gctcgtcgct
     7741 atcagcggcc agcttggccc accggtcgcg cggtgcaagc gagaccgccg gcatgtcctt
     7801 ccagcccgac cagctcaagc gaaagatgcg cccttgcttg ctgccgcccg tgccggatga
     7861 aaaatcgccg gtgctgtgcc aatccacgac atacagcgcg ccgtccgggc cggtgatcgc
     7921 ctgacacgga cgaaacagcg gatcgtcgga ctccagcaaa gcgaactgcg agatggccct
     7981 gtgcgtagcg tcgtcgggtt gcgtggcgaa agcccgcagc ctgtcgccgt tcgcggagat
     8041 caacagcccg ttggcgaaat tcacaaagga cactcccgtc gacgcagcgc tatccccgcg
     8101 aagcatgccg ggcagcgtcc ctgccagctc gccgccggcg gcagcgccgc cgccatgtcg
     8161 ccagccgtag ttgccgccct ccagcacgtg aatcagtcgg ccgccccctt ttccgaccgt
     8221 ccagtccaca tcggccagga agggatttcc cagcgggtca aacgtcaggc agccccgcgg
     8281 ctgacagaag ccgcgcgcga attcgcgaag ttgcgaacca tctggccgcg cgcgaaagac
     8341 ggccccgctg cgaagtaccg tcgcctgcga gccatcccag ctcctggcgc ggttgtcgct
     8401 ggtgccgcaa gcgacgtaca ggcagccatc cgggccaagc gacaggccga cggcctgaaa
     8461 atggatgaca cccgaaagtc cgctgatcag taactgctcc agccacttgc catcgccggc
     8521 caccgtcgtt ggcggtccgc gcttctcggt cgcggctgcc gccagtt
//
LOCUS       DASRJB010000321         6371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_941869, whole genome shotgun sequence.
ACCESSION   DASRJB010000321 DASRJB010000000
VERSION     DASRJB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941869"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFB80_08580"
     CDS             complement(<1..432)
                     /locus_tag="VFB80_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33862.1"
                     /translation="MFTSLFIAVLFAAELPPPIVALAVTPDGSQVLAGSQAGVQILSL
                     PELKAVGAIATKLEQVHELAFSPKGDALAISGGSPGERGAVELWHWPAATLHWSQPAG
                     NDVAYDVAWRPAGMRLAIAGADKSVTLGFTQEDVRPSATRLL"
     gene            complement(530..1804)
                     /locus_tag="VFB80_08585"
     CDS             complement(530..1804)
                     /locus_tag="VFB80_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33863.1"
                     /translation="MSNLLVQRAAAAPTAKAKRAILIWLDGGPSHLETFDVKPDAPRE
                     VRGPLVPVATNVPGIQVSECLPLLAQRMQHASIIRSMTSPLGEHNLGTHYLMTGYPPT
                     PALEYPAIGSVVAHLDRAERTLPRHIAVPNFGVGGRRYGGNGYLPEETRPFAVGGDPA
                     QPGFKVRDLDYYPGLAGGWIDRRREYLQRLDEFSRSVEQAASGGTPTRGAGAPVPATF
                     EQAYRLITSPAAKGAFDLSQESATTRGRYGPRTIGQSALLARRLVEADVPFVTINYPG
                     WDTHDNLYTRLKEGYTGAKVPVGLVPALDLALSALLDDLADRGLLAETLVMVMGEFGR
                     TPKLNTLGGRDHWPRVFSVLLAGGGVPGGQVIGSSDATGESPKDRPVTPADLAASIYT
                     LLGIDPQQLLHTADGRPVHVSRDGRVVKELVG"
     gene            complement(1946..4054)
                     /locus_tag="VFB80_08590"
     CDS             complement(1946..4054)
                     /locus_tag="VFB80_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33864.1"
                     /translation="MNLCFLLLLGAVPPPVDFDTDVLPVLTKAGCNAGACHGAAAGRG
                     GFKLSLFGGDPAADQRAIARELEGRRVNLAHPERSLLLLKPTWQLDHEGGERFTADGS
                     EAAVLLQWIRVGARRTGDKHLIRLEVTPAQTAFDRLPANYGLSALAQFSDGTQRDVSH
                     QATYTPADPAATEVDPRGGVKVQRPGRHSIVVRFLTETIAVQVTAAYPQPSLAADKLP
                     RNNWIDDEINATLTALRLPLSPQADEAALLRRVTLDLSGRLPPPERILAYLADRRSPQ
                     EKFAAEVERLLASPEFTEQWTYKLAEWLRIQPGPNDREGTEAYYAWVRKQVEGRRPLN
                     ELVAELITADGDSHLLGPPNFHRSAGDARGEAEHVAETLLAVRLRCANCHNHPLDRWT
                     QDDYHGLAAIFARLDRGREVKLKSSGEVAHPATGDAAIPRIPGAAILDPQADGRPRLA
                     AWLTSTDNPYFARAWVNRLWDSLLGRGLVHPVDDLRDTNPASHPALLERLTQDFVAAS
                     FDLRHTLRQIALSAAYQRSAATQAGNLHDDRFYSHALPRQLSQEVLLRAVCTATGVAE
                     HALPLSFAPPTGMVSDRPRQLPFDPLAVAVCAGQPGQARDALAQPPNDLAAQLHWLNG
                     PVVNAKLNDRASALAELSKSNAPAQQLIETYYLRTLSRLPTASEQQFWQRELAGGDWA
                     QKCQDFAWSLLSCREFVTNH"
     gene            complement(4105..4803)
                     /locus_tag="VFB80_08595"
     CDS             complement(4105..4803)
                     /locus_tag="VFB80_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN33865.1"
                     /translation="MTGTSSVHAVRPITGITIRVLDGADRGRVFQNLSPPITIGREEG
                     NTIQLNDERVSRYHVKIQEDHNRLVITDLESTNGSKVNGEDVQLRILRFGDMIQLGRS
                     VLLFGSREQIAQRLARLRADESDSRTPDPEVNKAANISSLDFELNWSEDADLQATIHA
                     LEPPELPERLTPGQAAQLAELLEFLHLRMRNLIGSVHGDGKSNKMTLDLRQWQALIDL
                     QARLSEYLRQVGEP"
     gene            5265..5540
                     /locus_tag="VFB80_08600"
     CDS             5265..5540
                     /locus_tag="VFB80_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33866.1"
                     /translation="MKKLLLTLPFVALPWLVAGCEESVEKAQQDVEQAHKNAAERVTD
                     TVRDADKVIVDEQRKVEDTAKEEAEKIKQEQRELEDAKRAAEKRNNP"
     gene            5669..5935
                     /locus_tag="VFB80_08605"
     CDS             5669..5935
                     /locus_tag="VFB80_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33867.1"
                     /translation="MRLVSKNGLAICLGAVLLGGCHAYVDTDGPPARVEVNPAPAPAP
                     APNIDVDVRPAPAPVPAPRVDVDVRPAPAPAPKVDVDVNVKRNP"
     gene            complement(5999..>6371)
                     /locus_tag="VFB80_08610"
     CDS             complement(5999..>6371)
                     /locus_tag="VFB80_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HZN33868.1"
                     /translation="ATYLQYTYARCCSIFRKGGFDRAALRAGGAKIVLRGPAERTLGL
                     QLLRFAEALADVEADYMPHHLTAYLFDLGKSYSTFFENCPVLKAESPAVKDSRLLLCD
                     LTARTIEQGLALLGIGVVDKM"
BASE COUNT         1110 a         2048 c         2149 g         1064 t
ORIGIN      
        1 caagagccgc gtggcgctgg gacgcacgtc ctcttgtgta aagcccagcg tgaccgattt
       61 gtctgcgccg gcgatggcca gccgcatgcc agccggccgc caggccacgt cgtaggccac
      121 gtcgttgccc gccggctgcg accagtgaag tgtggccgca ggccaatgcc agagctcgac
      181 cgcgccccgc tcgccgggcg agccgccgga aattgccaac gcgtcgccct tcggcgagaa
      241 cgccagctcg tgtacctgct ccagcttcgt cgcgatggcg ccgacggctt tcagctccgg
      301 cagcgagagg atctgcactc ccgcctgaga gcctgcgaga acctgggagc catccggcgt
      361 caccgccagc gcgacgatcg gcggcggcag ttcagcagca aagaggaccg caatgaacag
      421 cgatgtgaac aagtccgtct gagtcctgtg cttcgtactc agtactcagt acggagtacc
      481 aagtactgat cgggcccaat cgacggggcc agctttgaac cttgaacttc tacccgacga
      541 gctccttcac cacccgcccg tcgcggctga cgtgcaccgg cctgccgtcg gcggtgtgca
      601 gcagttgctg cgggtcgatc cccagcagcg tgtaaatcga ggcggcgagg tcggcgggcg
      661 taacgggccg gtccttcggg ctttcgccag tggcgtcgct gctgccgatg acttgcccgc
      721 cgggcacgcc gccaccagcc aacagcacgc tgaagacccg cggccaatgg tcgcgacccc
      781 ccagtgtgtt gagcttcggc gtgcggccga actcgcccat caccatcacg agcgtctccg
      841 cgaggaggcc gcggtcggcc aggtcgtcga gcagcgccga cagggccagg tcgagcgccg
      901 gcaccaggcc cacgggaact ttcgcgccgg tgtagccttc cttgagccgc gtatagaggt
      961 tgtcgtgcgt atcccagccg ggatagttga tcgtcacgaa gggcacgtcg gcctcgacca
     1021 gccggcgggc cagcaaggcc gactggccaa tggtccgcgg gccgtaccgc ccgcgcgtcg
     1081 tggccgattc ctgcgacagg tcgaacgcac ccttggcggc gggcgacgtg atcaggcgat
     1141 aggcttgctc aaacgtagca ggcaccgggg cacccgcacc gcgcgtcggg gtgccgccgc
     1201 tcgctgcctg ctctacgctg cgcgaaaact catccagccg ctgcaggtac tcgcggcggc
     1261 ggtcgatcca cccgccggcc aggccgggat agtagtcgag gtcgcggact ttgaagccgg
     1321 gctgtgccgg gtcgccaccg accgcgaagg gccgcgtttc ctcgggcaga taaccgttgc
     1381 cgccatagcg cctgccgcca acgccgaaat tggggacggc gatgtgccgc ggcagcgtcc
     1441 gctcggcgcg gtccaggtgg gcgacgacgc tgccgatggc cgggtactcg agagccggcg
     1501 tgggcggata gccggtcatc aggtagtgcg tgcccaagtt gtgctcgccc agcggcgagg
     1561 tcatcgagcg gatgattgac gcgtgctgca tccgctgagc aagcagcggc aagcactccg
     1621 aaacttgaat gccagggacg ttggtggcaa ccggcaccag tgggccacgc acctcgcgcg
     1681 gggcgtcggg cttgacgtcg aacgtctcga gatggctcgg gccgccgtcg agccaaatca
     1741 agatcgcccg cttggccttg gccgttggtg cagccgctgc acgctgcacc agcagattcg
     1801 acaaactaag acccagtgcc gtcagccctc cgatgcgaac gaaatcgcgt cgcgaaggac
     1861 gatttgggat tgaggatttg ggattgggga attccgtcat ggcatgcgtc tttcaacgga
     1921 cgactgtcaa ctgacaactg acatgctaat ggttcgtcac aaactcgcgg cacgaaagca
     1981 ggctccaggc aaagtcctgg catttctgcg cccaatcgcc gccggccaat tcccgctgcc
     2041 agaactgctg ctcgctcgcc gtgggcaggc gcgagagcgt gcgcaggtaa taggtctcga
     2101 tcagctgctg ggccggcgcg ttcgacttcg acagctccgc tagggcgctg gcacggtcgt
     2161 tcagcttggc gttgacgacc gggccgttga gccagtgcag ctgcgcggcc aggtcgttgg
     2221 gaggctgggc cagcgcgtcg cgggcttgtc cgggctgacc ggcacacacg gcaaccgcca
     2281 gcggatcgaa cggcagttgc cgcggccgat cgctaaccat ccccgtcggc ggcgcaaagc
     2341 tgagcggcaa ggcatgctcg gcgacaccag tcgcggtgca aaccgcacgc agcaggacct
     2401 cctgcgaaag ctgccgcggc agcgcgtgcg aataaaaccg atcgtcgtgc aggttgccgg
     2461 cctgggtcgc cgcgcttcgc tgatacgcgg ccgaaagcgc gatttgccgc agcgtgtgcc
     2521 gcaggtcaaa acttgcggcc acaaaatcct gagtcagccg ctcgagcagc gccgggtgac
     2581 tcgcgggatt ggtatcgcgg aggtcatcga ccggatgcac caggccccgg cccagcagcg
     2641 aatcccagag gcgattgacc caggcccgcg cgaaatacgg attgtccgtc gaagtcagcc
     2701 aggcggcgag ccgcggccgg ccgtcggcct gagggtcgag aatcgccgcg cctggaatcc
     2761 gcggaatcgc cgcatcgccc gtggccgggt gagcgacctc gccgctggat ttcagcttga
     2821 cttcgcggcc gcggtcgagc cgggcgaaga tcgccgccag cccgtgatag tcgtcctgcg
     2881 tccaacggtc gagcggatgg ttgtggcagt tggcgcagcg caggcgaacg gccagcagcg
     2941 tctcggcgac gtgctcggcc tcgcctcgcg catcgcccgc actgcggtga aaattcggcg
     3001 gtccaagcag atgcgagtca ccgtcggcgg taatcagctc ggccacgagc tcgttcagcg
     3061 ggcggcggcc ctcgacttgc ttgcgcaccc acgcgtaata cgcctcggtt ccctcgcggt
     3121 cgttcggccc cggctgaatc cgcagccatt cggccaactt gtacgtccac tgctcggtga
     3181 attccggcga cgcgagcagt cgctcgactt cggcggcaaa cttctcctgc ggcgagcggc
     3241 ggtcggccag gtaggcgaga attctctcgg gcggcggcag gcggccggac aggtcgagcg
     3301 tgacccgcct gagcagcgcc gcctcgtcgg cctgcggcga aagcggcagg cgcagggctg
     3361 tcaaagtggc attgatctcg tcgtcgatcc agttattgcg cggcagcttg tcggccgcga
     3421 gcgacggttg cggataggcg gccgtgactt gaacggcgat cgtttcggtc aaaaagcgga
     3481 cgacgatgct gtgccggccc ggccgctgca ccttgacgcc gccgcgcgga tcgacttcgg
     3541 tcgccgccgg atcggccggc gtgtaggtgg cctgatggct cacgtcgcgc tgcgtgccgt
     3601 cgctgaactg cgccagggcg gacagaccat agttggctgg caagcggtcg aacgcagttt
     3661 gagccggcgt gacttcgagc cggatgaggt gcttgtctcc ggtgcgccgc gctccgacgc
     3721 gaatccactg cagcaagacg gccgcctcgg aaccgtcggc cgtgaaccgc tcgccccctt
     3781 cgtggtccag ctgccacgtc ggcttgagca gcagcagact tcgctcggga tgagccagat
     3841 tcacgcggcg gccttccaac tcgcgcgcga tcgcccgctg atctgccgcc ggatcgccgc
     3901 caaacagcga caacttgaat ccgccgcggc cggcggcggc cccgtgacac gcccccgcgt
     3961 tgcagccggc cttggtcaat accggcagca cgtccgtgtc gaaatcgacc ggcggaggga
     4021 cggcgccgag cagcagcagg aagcagaggt tcacaactcc agagtatatc gcacggcgaa
     4081 agatgacgca ccaaaacaaa ccggctacgg ttcgccgact tgccgcagat actccgacag
     4141 ccgggcctgc aaatcgatca gggcctgcca ctgccgcagg tcgagggtca tcttgttgga
     4201 cttgccgtcg ccgtggacgg agccgatcag gttccgcatc cgcaggtgca ggaattccag
     4261 gagctcggcc agctgcgccg cctggccggg cgtcagccgc tcgggcagct ccggcggctc
     4321 caaggcgtgg atcgtggcct ggagatcggc gtcttcgctc cagttcagct cgaagtcgag
     4381 cgacgagatg ttcgctgcct tgttgacctc gggatcgggc gtgcggctgt cgctttcgtc
     4441 ggcccgcaat cgagccagcc gctgggcgat ctgctcgcgc gatccgaaca agagaacgct
     4501 gcggccgagc tggatcatgt cgccgaagcg gaggatgcgc agctgcacgt cctcgccgtt
     4561 gaccttgctg ccgttggtgc tctccaggtc ggtgatcacc agtcggttgt ggtcctcctg
     4621 gatcttgacg tggtaccggc tgacccgctc gtcgttgagc tgaatcgtgt tcccctcttc
     4681 gcggccgatg gtgatcggcg gcgacaggtt ttgaaacacg cgtccccggt ccgcgccgtc
     4741 gagcacgcgg atggtaatgc cagtgatggg cctgacggcg tgaacagaag aggtgccggt
     4801 catgctgctc ctaatggcgc ttataacatc gactcaaaag cggggtcaag tcgctccatg
     4861 gcctcctgat tcgcgggttt ttcggggctc gccggcagcc gcccgtcatc cataacgccg
     4921 caccctgtag cacatccagt gccgttgatt ctccatagtc ttgtttcggc caaatttccg
     4981 tcacggctga ttctgaccgg aatgggcaca ttttgaccgg cggacgacca ccgggggccg
     5041 cctccatacc cgagcgccaa gcgagatttc ggcctttggc aagttggcgc gcaagtccgt
     5101 ggcggttctc gactaagggc cggcgaggcg acgcaatcgg gagctggcga gccagtggcg
     5161 gcccgtgatt tgcctctagc tctttccatt caacctgcca caccacgacc ggtgcctgga
     5221 ttcagtcgct gccggtcgtc caagacatcg aagggagatg cgtcatgaag aagctgctac
     5281 tcacactgcc gtttgttgcg ctgccgtggc tcgtggccgg ctgcgaggaa tcggtcgaga
     5341 aggcccagca agacgtggag caagcgcaca agaacgcggc cgagcgcgtg acggacacgg
     5401 tccgcgacgc agacaaggtc atcgttgacg aacaacgcaa ggtggaagac accgccaagg
     5461 aggaagcgga gaagatcaaa caggagcagc gcgagctgga ggacgccaag cgggcagccg
     5521 agaaacggaa caacccgtaa acgcggacgt accaacggtg agaggggagc cacgggtgaa
     5581 ctcggactgg cacgggtgaa gtgagagtga gttcgtgtcg tccggttgat tcgtggctca
     5641 tttgcttcaa ttaaaaaagg agctcgaaat gcgtttggtt tccaaaaacg ggttggcgat
     5701 ctgtcttggc gcggtgctgc ttggcggctg ccacgcctac gtcgataccg acgggccgcc
     5761 cgcgcgggtg gaggtgaatc ctgcgcccgc tcccgcgccg gcgccgaata ttgatgtgga
     5821 tgtccggccg gctcctgctc cggtgccggc gcccagggtt gacgtcgatg ttcggccggc
     5881 tccagcgccg gcgccgaagg ttgacgtcga tgtgaacgtg aagcggaatc cgtagacaaa
     5941 acggaattat cccggagccg gaggcgatgg cgcttggggc agcctccggc tccggctatc
     6001 acatcttgtc cacgacgccg atgcccagca gcgcgagtcc ctgctcgatc gtccgcgcgg
     6061 tgaggtcgca gagcagcagc cggctgtcct tgacggcggg cgattcggcc ttgagcacgg
     6121 ggcagttttc gaagaaagtc gaataactct tgccgaggtc gaagagatac gccgtgaggt
     6181 ggtgcggcat gtagtcggcc tcgacgtcgg ccagagcctc agcgaagcgg agcagttgaa
     6241 gacccagagt tcgctccgca ggacccctta gcacgatctt cgctccaccg gcccgcaagg
     6301 cggcgcggtc aaagccaccc ttgcgaaaga tgctgcagca gcgggcgtag gtgtattgca
     6361 ggtacgtggc c
//
LOCUS       DASRJB010000322         7020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2629431, whole genome shotgun sequence.
ACCESSION   DASRJB010000322 DASRJB010000000
VERSION     DASRJB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629431"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="ftsH"
                     /locus_tag="VFB80_08615"
     CDS             <1..1272
                     /gene="ftsH"
                     /locus_tag="VFB80_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZN33869.1"
                     /translation="GKTLLARAVAGEAGVPYYSVNGSEFIQMFVGVGASRVRDLFRTA
                     KENAPAIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQILSEMDGFAQNDFVIVLAATN
                     RPDILDPALLRPGRFDRHVTVGRPTQKGRVEIFKVHTRDVPLADDVDLETLGAATIGL
                     TGADIRNIVNEAALWAARNNKSQVEMSDLEYARDKVLMGAKREEVLTEKEKEKTAYHE
                     AGHTLLAWQLPGAYRVHKVTIVPRGRTLGVTQMLPSEDRVSMSEPELNDHLTTLLGGR
                     AAEKLIYNETTAGAENDLERATGLARRMVMNWGMSDRVGPVSYKVADEDPFLGREMHQ
                     QRQFSEHTLQLIDGEVGRILREQSDRATRMLTEQRGRLEKLAEALLAKEELDDNEIEE
                     ILGKSVHKKKAKPQVEIAPGGRAPDIGLAPA"
     gene            1942..4236
                     /locus_tag="VFB80_08620"
     CDS             1942..4236
                     /locus_tag="VFB80_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN33870.1"
                     /translation="MAALLAAPVAAAEETGEQIFKTLCAKCHGDKGQGVEEHHPDPLV
                     GERPLDELAALIDKTMPEGEPEKVSPEQAQKVATYIYDAFYSPLAQARNQPARIELSR
                     LTVRQYQNTVADLIGSFRSPGKWDLERGLEAEYSDRVGRRGRRGGGGSPALKRVDPQV
                     KFDFGEESPVEGKLEAHRFAIKWQGSVLAPDTGEYEFIVRTEHATRLFVNDTEQPLID
                     RLVKSGSDTEFRASIYLIGGRAYPLVLEFSKGKTGVDDSAKQKAPPPKVKASIALLWK
                     QPGRAEEVIPQRNLSPHTNPEQFVLTTKFPPDDRSLGYERGSAISKEWDSAATDAAIE
                     TAAYVATNLRELAGVSADDDSAAQQLKDFGVRFVERAFRRPLTGEQKAFFVERQFEDA
                     PDPAGAIKRVVLLALKSPRFLFREPGAGGDGYDAAARLSFSLWDSLPDEPLLKAAAQN
                     ELRTREEVAAQAERMIADLRTRGKVREFLIQWLKVDRVLDLSKDKELYAEFSPQVISD
                     LRTSLDLLLDDVVWSAASDFRQLLLTDGIFLNGELAKVYRAELPDGAPFEKVRLDPEH
                     RAGILTHPYLLAGFAYTSASSPIHRGVFVSRSLLGRSLRPPPEAFTPLAPELHPDLTT
                     RERVALQTNSKNCQSCHAMINPLGFTLENFDAIGRFRTEEKGKPIDAKGGYLTRTGER
                     VKFSGVRDLAEFLAASDETHSAFAGQLFHYLVKQPVRAYGPTKHAELKQSFAQNEFSV
                     RKLIAEIAVTAALAQPEKIAHTSP"
     gene            4295..5605
                     /locus_tag="VFB80_08625"
     CDS             4295..5605
                     /locus_tag="VFB80_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN33871.1"
                     /translation="MLDRTRREFLRDLGISAAALPFVLNLPSLARAGEQPAKKRLVVM
                     FSPNGIVPKTFWPDEEGAEFTLKESLQPLAPLKDKTLILHGVCDRIRGDGDAHMRGIG
                     CLLTGTELFPGNVQGGSDTPAGWASGLSIDQEIKNFLQASSETRTRFGSLEFGVLVPD
                     RADTWTRMVYAGPNKPVAPIDDPYQMFTKLYGRQKDQASLKSILDDLQEDLQKVKSVV
                     SAADRRLLDEHTTLVRELEQELKADRASVGHAVPEIEPGVKEDNDNIPKITKLQIELL
                     VNSFQADFARLATLQFTNSVGGARMRWLGVEKGHHELSHEPDSNEEVQAQLTKINRWY
                     CEQMAYLAQRLAAIPEPGGSGSLLDNTTIMWTNELGKGNSHTLDNIPFVLVGGGLGFQ
                     MGRSRKFRRQPHNRLLLALAHGFGHHLEKFGNPNYCESGPLNLS"
     gene            complement(5619..5822)
                     /locus_tag="VFB80_08630"
     CDS             complement(5619..5822)
                     /locus_tag="VFB80_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZN33872.1"
                     /translation="MIKLTRQSGEVFVLNAELIRYIETGAGTFITLTTGERLIVREPL
                     DEVMQRAVRYQQSKFLLPLPRAA"
     gene            complement(5828..>7020)
                     /locus_tag="VFB80_08635"
     CDS             complement(5828..>7020)
                     /locus_tag="VFB80_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HZN33873.1"
                     /translation="ISNLATPGNAALDFDGPEVASNTLLVNVIRRDGLDYENLFQEGT
                     LEFTPSKGGRTLGTQEFQITSTSTVQDLLDFMQEALGIQTSIDDPANPIPGSVNNIPG
                     ESGTLAAGLTIQDGQLRVVSNNGVDNAVDIDSSAFRIRTTTGDILTPNLAFDSVQDAA
                     GQSAVADFIAYDTLGIPINVRVTAVLESLTDSSTTYRWFAESADNSPLSGADTSLGTG
                     LITFDGEGRLIGTSNNTVTVERRNLPSSDPLEFDLDFSQVTALASDNSELSAARQDGS
                     PAGTLTSFIIGEDGLIRGVFASGVTRDLGLVRLARFANPVGLVQRGQNLFSQGINSGL
                     PIEGDPNTQGIGTILAGSVELSNTDIGKNLIDLVLATTMYRGNARVISTAQQMLDELL
                     NLRR"
BASE COUNT         1307 a         2279 c         2279 g         1155 t
ORIGIN      
        1 ggcaaaacgc tgctggctcg cgcggtggcc ggcgaggcgg gcgtgccgta ttactccgtg
       61 aacggctcgg agttcattca gatgttcgtg ggggtcggcg cgagccgcgt ccgcgacctg
      121 ttccgcacgg cgaaagaaaa cgccccggcg atcatcttca tcgacgagat cgatgccgtc
      181 ggccggcagc gcggcgcggg gctcggcggc ggccacgacg agcgcgagca gacgctcaac
      241 cagattctca gcgagatgga cggttttgcc cagaacgact tcgtgatcgt gctggcggcg
      301 acgaaccggc ccgacatcct cgatccggcg ctgctgcggc ccggccgctt cgaccggcac
      361 gtcactgtcg gccgtccgac gcaaaagggc cgcgtggaga tcttcaaggt ccacacccgc
      421 gacgtgccgc tggccgacga tgtcgatctg gaaacgctcg gcgcggcgac gatcggcctg
      481 acgggcgctg acatccgcaa catcgtcaac gaagccgccc tgtgggccgc ccgcaacaac
      541 aaatcgcagg tcgagatgag cgacctggag tacgcccgcg acaaggtgtt gatgggcgcc
      601 aagcgcgagg aagtgctgac cgaaaaggaa aaggaaaaga ccgcctatca cgaagccggc
      661 cacacgctgc tggcctggca gctgccgggc gcctaccgcg tgcacaaggt cacgatcgtc
      721 ccccgcggcc gtacgctcgg cgtgacgcag atgctcccct cggaggaccg cgtgagcatg
      781 tcggagccgg agctgaacga ccacctcacg acgctgctcg gcggccgggc ggcggagaag
      841 ctgatctaca acgaaacgac cgccggcgcc gagaacgatc tggagcgggc gaccggcctg
      901 gcccggcgga tggtgatgaa ctggggcatg agcgaccgcg tcggcccggt cagctacaag
      961 gtggccgacg aggacccctt cctcggccgc gagatgcacc agcagcggca gttcagcgag
     1021 cacacgctgc agctcatcga cggcgaggtc ggccgcatcc tccgcgagca gtccgaccgc
     1081 gccacacgaa tgctcaccga gcagcggggc cggctcgaaa agctcgccga ggcgctcctg
     1141 gccaaggaag aactcgacga caacgagatt gaggaaatcc tcggcaagtc ggtccacaaa
     1201 aagaaggcca agccgcaagt cgaaatcgcc cccggcggcc gggcccccga catcggcctg
     1261 gctccggcct gacgctgctc gatttaccgc aaagaggcca gcagccgagg cgcacaggct
     1321 ttacaaccac gcttcgtgcc ctaaaatgct cgcgtgtacg aaataatact cggccgtcgc
     1381 gcatcgtatg gttggtcttc gatgcagcgc acggtggcta tcgtgcataa cctgcataat
     1441 gcagtgcctt ccgcattgct gtaagtcctt atttcacaat cctttacaac caatcatgct
     1501 cgccaggatt ttccttagtc ctgcatatgc atctgcggtg tgcccgccgc tttgcgcctt
     1561 tagcatctcc gcgactttgc gttaaatcga gcaacggatg gccaagagtc actcttccaa
     1621 tacccatgaa tccagacgcc ttaagctgca taaactgcat atccaatgca ctaatatttc
     1681 gtcgcaactc attgatccac aaggccttgc gttgcccctg acgtcgagag aaaagaattc
     1741 gttatgcagc ataaggacga ccgcgcgttg accctcccgg caggccgcgg ataagctgtc
     1801 tgatggaatg agttggcgct tggggactcg ttccaatcct gcctgttcgc cgcttgccgg
     1861 tctcgccagt tttcgcctag cgccatgact ttccattact cctccattcg gcggcttgcg
     1921 ccctggttgc tgtccctcgg aatggcggcc ttgcttgccg caccggtcgc ggctgcggaa
     1981 gagacgggcg agcagatttt caagacgctg tgcgccaagt gccatggcga caaagggcag
     2041 ggggtcgaag agcaccaccc cgatccgctg gtgggcgagc ggcccttgga cgagctggcg
     2101 gcgctgatcg ataagacgat gcccgaaggc gagccggaga aggtcagccc tgagcaggcc
     2161 caaaaagttg caacctacat ctacgacgcc ttttattcgc cgctggccca ggcccgcaat
     2221 caaccggccc ggatcgagct gtcgcgcctg accgtacggc aataccagaa cacggtcgcg
     2281 gacctgatcg gcagctttcg cagccccggc aagtgggacc tcgagcgcgg cctggaggcc
     2341 gagtactccg atcgcgtcgg ccggcgcggc cggcggggcg gcggcggttc gccggccctg
     2401 aagcgcgtcg atccgcaggt gaagttcgac ttcggcgagg aaagcccggt agagggtaag
     2461 ctggaggctc accgcttcgc gatcaagtgg cagggctcgg tcctggcccc cgacacgggc
     2521 gaatacgagt tcatcgtccg caccgagcat gccacgcggc tgttcgtcaa cgacaccgag
     2581 cagccgctga tcgaccggct ggtgaagtcg ggcagcgaca cggagttccg ggcgtcgatt
     2641 tacctcatcg gcggccgggc gtatccgctg gtgctggagt tcagcaaggg gaagacgggc
     2701 gtcgatgact cggccaagca gaaagcgccg ccccccaagg tgaaggcgag catcgccctg
     2761 ctctggaaac agccgggacg cgcggaggag gtgattccgc agcggaatct ctcgccgcac
     2821 accaatcccg aacaattcgt gctgacgaca aaattcccgc ccgacgaccg cagcctgggc
     2881 tacgagcggg gctcagcgat ctcgaaggaa tgggactcgg ctgcaacaga cgccgccatc
     2941 gaaacggctg cctatgtggc cacgaacctg cgcgaactgg ccggagtgtc ggccgacgac
     3001 gacagcgcag cccagcagct caaggatttt ggcgttcgct tcgtcgagcg agccttccgc
     3061 cggccgctga ctggcgagca gaaggcattc ttcgttgagc ggcaatttga ggacgcgccc
     3121 gatccggcag gggcgatcaa gcgcgtcgtc ctgctggcac tcaagagccc gcggttcctg
     3181 ttccgcgagc ccggcgcggg cggcgacggc tacgacgcgg ccgcccgact gtcgttcagc
     3241 ctgtgggact cgctgccgga tgagccattg ctgaaggccg cggcgcagaa cgaacttcgc
     3301 acgcgcgaag aggtcgcggc gcaggccgag cggatgatcg ccgacttgcg gacccgcggc
     3361 aaagtccgcg agttcctgat ccaatggctc aaggtcgatc gcgtgctcga cttgtcgaaa
     3421 gacaaggagc tgtatgccga gttcagcccg caggtcatct cggacctccg cacgtcgctc
     3481 gatttgctcc tggacgacgt cgtctggagc gcggcctccg atttccgcca gctcctgctc
     3541 acggacggca tttttctgaa cggtgagctg gccaaggtct atcgggccga attgccggat
     3601 ggcgcgccgt ttgaaaaggt gcggctcgat cccgagcacc gggcgggcat cctcacgcat
     3661 ccgtacttgc tggccggatt tgcctatacg tccgccagct cgccgattca ccgcggtgtg
     3721 ttcgtctcgc gcagcctgct gggccggtcg ctgcgcccgc cgccggaagc cttcacgccg
     3781 ctggcccccg agctgcatcc agacttgacg acccgcgagc gcgtcgcgct gcagaccaac
     3841 tccaagaact gccagagctg ccacgcgatg atcaacccgc tcggcttcac gctggagaac
     3901 ttcgacgcca tcggccgctt ccgcaccgaa gagaagggca agccgatcga tgccaaaggc
     3961 ggctacctga cgcggaccgg cgagcgcgtc aagttcagcg gagtccgcga cctggccgaa
     4021 ttcctcgccg ccagcgacga gacgcacagc gccttcgccg gacagctgtt ccactatctc
     4081 gtgaagcaac ccgtgcgggc gtacggcccg accaaacacg ccgaactcaa acagtcgttc
     4141 gcccaaaatg aattcagcgt ccgcaagctg attgccgaga ttgccgttac cgccgcgctt
     4201 gcacagccgg agaagattgc ccacactagc ccgtagcacg cgactccgtc gcgtgccttc
     4261 ccgccacgga gtggcgggtt acaaggaaac catcatgctc gaccgcaccc gccgtgaatt
     4321 cctccgcgat ctgggcatca gcgccgcggc actgccgttt gtgctgaacc tgcccagcct
     4381 cgcgcgggcg ggcgagcagc cggcgaagaa gcggctggtc gtgatgttca gccccaacgg
     4441 gatcgtgccc aaaacgttct ggccggatga ggaaggggcc gagttcacgc tcaaagagag
     4501 cctgcagccg ttggcgccac tcaaggataa gacgctgatt ctgcacggcg tctgcgaccg
     4561 gattcgcggc gacggcgacg cccacatgcg gggcatcggc tgcttgctca ccggcaccga
     4621 gctgttcccc ggcaacgtgc agggcggcag cgatacgccg gccggctggg ccagcggttt
     4681 gtcgatcgac caggagatca agaacttcct gcaggccagc tccgaaaccc gcacgcggtt
     4741 cggctcgctc gagttcggcg tgctggtgcc cgaccgggcc gacacctgga cccgcatggt
     4801 ctatgccgga ccgaataagc cggtcgcccc gatcgacgac ccgtaccaga tgtttacgaa
     4861 actctatggc cggcagaagg accaggcgag cctgaagagc atcctcgacg acttgcagga
     4921 ggatttgcag aaggtaaaga gcgtggtcag cgccgccgac cgccgcctgc tcgacgagca
     4981 cacgacgctg gtccgcgagc tcgagcagga gctgaaggcc gaccgggcga gcgtgggcca
     5041 tgcggtgccc gagatcgagc cgggcgtcaa ggaagacaac gacaacatcc ccaagatcac
     5101 gaagctgcag atcgagctct tggtgaacag ctttcaggcc gatttcgccc gcctggcgac
     5161 gctgcagttc acgaactccg tcggcggggc gcggatgcgc tggctgggag tcgagaaggg
     5221 gcatcacgag ctgtcgcacg agcccgacag caacgaagag gtgcaggccc agcttaccaa
     5281 gatcaaccgc tggtactgcg agcagatggc ctacctcgcc cagcggctgg ccgctatccc
     5341 agagcccggc ggcagtggca gcctgctcga caacacgacc attatgtgga ccaacgagct
     5401 gggcaaaggc aactcgcaca cgctcgacaa catcccgttc gtgctggtcg gcggcggcct
     5461 cggcttccag atgggccgct cccgcaagtt ccgccgccag ccgcacaacc ggctgctgtt
     5521 ggccctggcc cacggcttcg gccaccacct cgagaagttc ggcaacccga actactgcga
     5581 gagcggcccg ctgaacctga gctgaccgcc gtgccccctt acgccgcccg cggcagtggc
     5641 agcaggaatt tcgactgctg ataccgcacg gcccgctgca tgacttcgtc gagcggctcg
     5701 cggacgatca gccgctcgcc cgtcgtgagc gtgatgaacg tgcctgcgcc ggtttcgatg
     5761 tagcgaatca gctcggcgtt gaggacaaag acctcgccgc tttgccgcgt gagtttgatc
     5821 atcggattta ccgccgcaga ttgagcagtt cgtccagcat ctgctgggcc gtgctgatca
     5881 cgcgggcgtt gccgcggtac atcgtggtgg ccagcacgag gtcgatcagg ttcttgccga
     5941 tgtcggtgtt ggacagctcg accgagccgg cgaggatcgt gccgatgccc tgcgtgttgg
     6001 gatcgccctc gatcggcaag ccggagttga tgccttgcga gaacaggttc tggccgcgct
     6061 gcacgaggcc caccgggttg gcgaaccgcg ccaagcggac caggcccaag tcgcgggtca
     6121 cgccgctggc aaagacaccg cggataagcc cgtcctcgcc gatgataaag ctcgtgagcg
     6181 ttccggccgg cgagccgtcc tgccgcgcgg cggaaagctc gctgttgtcg gaggccaggg
     6241 cggtgacctg cgagaaatcg aggtcgaact ccagcggatc ggacgacggc aggttgcggc
     6301 gctcgaccgt gaccgtgttg ttgctggtgc cgatgagccg gccttctccg tcgaacgtga
     6361 tcaggcccgt gccgagcgac gtgtccgcgc cgctgagcgg gctgttgtcg gccgattcgg
     6421 cgaaccagcg ataggtcgtg gagctgtcgg tcaggctctc cagcacggcc gtcacccgca
     6481 cgttgatcgg gatgccgagg gtgtcgtagg cgatgaagtc ggccacggcg ctttggcccg
     6541 ccgcgtcctg gacgctgtcg aacgccagat tcggcgtcag gatgtcgccg gtcgtggtgc
     6601 ggattcggaa ggccgacgag tcgatatcga ccgcgttatc gacgccgttg ttggagacca
     6661 cgcgaagctg gccgtcctgg atcgtcaggc ccgcggcgag cgtgcccgat tcgcccggga
     6721 tgttgttcac cgagccggga atcgggttgg ccgggtcgtc gatcgaggtc tggatgccga
     6781 gcgcctcctg catgaaatcg aggaggtcct gcaccgtcga agtcgaggtg atctgaaact
     6841 cctgggtgcc cagcgtgcgg ccgcccttgc tgggcgtgaa ttcgagcgtg ccttcctgga
     6901 acaggttttc gtagtccagg ccgtcgcggc ggatcacatt gaccagcagc gtgttgctgg
     6961 cgacctccgg cccgtcgaaa tcgagagccg cgttgccggg cgtcgccagg ttagagatgt
//
LOCUS       DASRJB010000323        14475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1502706, whole genome shotgun sequence.
ACCESSION   DASRJB010000323 DASRJB010000000
VERSION     DASRJB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502706"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFB80_08640"
     CDS             complement(<1..547)
                     /locus_tag="VFB80_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33874.1"
                     /translation="MKPRKLKTVKHYHEPGDLHELTFSCFHQMKLLTNDAWRRSLSQS
                     INEAGGHLRFQLVAFVFMPEHVHLLVFPLAEEPALDAYLAGVKRPVSANAKGDLQQAN
                     SPLLRRLTVRERPGKYAFRFWQEGPGYDRNLQTPAAVMGSIEYLHLNPVRRKLVTQAR
                     LWKWSSARFYESDGQDQDPDLP"
     gene            complement(679..2004)
                     /locus_tag="VFB80_08645"
     CDS             complement(679..2004)
                     /locus_tag="VFB80_08645"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN33875.1"
                     /translation="MSRQLAPIFTAVFVGLLSSAASAQENWRSLFNGKDLSGWVNVNL
                     APTTFSVKDGLIVSTGVPTGTIRSERMYENFILELEWRHLKAGGNAGVFIWGDPLPAV
                     GTPFSRGIEVQILDGRTADNYTSHGDVFSIWGASMKPDRPHPGGWERCLPSEQRCKPS
                     PEWNHYRITCKDGVLKLAVNGKEVSGGSNCRPRKGYICLESEGSECHFRNLRIAELPS
                     TSPPPGEIAAEAAGFVPLYTGLDLAGWRQPAGHAGHWQPKNWTLHYDGHSEAAEPKEK
                     HLWTEKEYGDFQLIVDWRLPAKPTKKTYPVVLPSGDDKLDANGQVVMQEVDDAGNSGI
                     LLRGREDAQVNICCYPVGSGEVTAFRTNKQLAPAVRAAVTPKERADAPLGKWNRFLIT
                     LKGDRVSVELNGKEIVTDAQLPGIATRGPIGLQHHNEVVEFANLFIREL"
     gene            2138..2605
                     /gene="ribH"
                     /locus_tag="VFB80_08650"
     CDS             2138..2605
                     /gene="ribH"
                     /locus_tag="VFB80_08650"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZN33876.1"
                     /translation="MPQVFDGKSGSASGRFAIVVSRYNEHITAKLLAGAVETLGAGGV
                     ADGAIDVAWVPGAWEIPLVAAKLANSQRYVAVLCLGAVIRGETTHDEHINRQVSQSLG
                     QIALETGLPVLFGVLTCNTLEQAIHRAGGDAGNKGQECAEAALEMVRLLGKLS"
     gene            2611..4695
                     /locus_tag="VFB80_08655"
     CDS             2611..4695
                     /locus_tag="VFB80_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33877.1"
                     /translation="MSSICPIRPIAVFIGLALYSTAAAQEPKPDPFTQARRGLIARQV
                     LAAATRLFDERKWAEAEKLLEGATAKDSATPTDFYNLACAQARQGKTAAALDSLEAAV
                     AKGFRQATEMAADADLASLRGEERFQAALAKCKELAAKPAAQSKLEPLLVGKEGLAKV
                     GPENTVWLPQFDLFQTAFRFADNDPRIAEPAVTEHGKTGEQIRRWQKEGTAAGLFGIL
                     YDNRDGAHSQLPLSQFPQLTAALYGPEAKADNLDKGLAARFFFNRPTFGNSSTALTGG
                     PFWRSQTRLAQVDSRAAALLYEQYVKNALYFYPEHRDYDAGRNGPDGYGDVYPANTPY
                     VITSAGSSGSDQSFLHAVACTLAAFPPKTQELLIQRGLLMPTVQMILRRASVGSDAEY
                     LTGKAHPVVFRERQLNVERMVLLAHEMQPDAVPPMIQLQVVSEDQPRKGSDYFEAANL
                     GERIFDTPAAIARVWRTTARERRMILTAATSYDANNQPLKFHWAALQARPGSVTIKPL
                     KEDGSLVELRALWHERFASADGELETNRIDIAAFAHNGRYYSAPGFVTFFCLDDEERK
                     YDEQGRIASVAYRSPAKGGNYADPMTHTLRDWRDEYQYDAAGRLTGWTRVRGEQREYF
                     TAHGHLISQKDDKGRARTARKVSYVPKAAQPGELATLEVVADAELLTYAYDGEDDRVG
                     HIADQLPDAKTR"
     gene            4794..5339
                     /locus_tag="VFB80_08660"
     CDS             4794..5339
                     /locus_tag="VFB80_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33878.1"
                     /translation="MNGLVTLTSAALLLAWNAIAPGNVAPPPKKPERVVAEVKIERGA
                     IKGEGRAVQAKIIIPKGLVHGGAAGAVPVVPAPGAAPAPRAVPAPNARDQAGLPFGTI
                     IAGVCMSLAAVSLVFVVRGNRGAKTTALAIVAGALVLGAFSAAYADLIPGRPNGPVPV
                     PAEIVIELVDGGDTVTLQLAR"
     gene            5431..6456
                     /locus_tag="VFB80_08665"
     CDS             5431..6456
                     /locus_tag="VFB80_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33879.1"
                     /translation="MATATLDDVAPPRAESPARSALVFAAAATAVIAASCLLAGFAPL
                     GFSIVTVFLFAGPHNWLEARYMLTRMPARWGPLTLYFTTGIAGVLLLAAGLAAVRWLG
                     EWLRLDSEMWLALYAGWNSVFVAWVAALALMRSRQNPRRNWLWIVPGAFVLIALNWLW
                     PMTWSLALVYLHPLVALWFLDREIGKMQPAWRTAYRGCLLLVPAALGVLWWNLASAPN
                     LPGNDWLQQQITQHAGAGILSGVSTHLLVSTHTFLEMLHYGVWIVAIPLVSVRMTPWQ
                     LDNVPLARRSNAWRAAILAAVAAGVIVMLVLWGGFVANYPLTRDIYFTVAMLHVLAEV
                     PFLLRLL"
     gene            6464..6880
                     /locus_tag="VFB80_08670"
     CDS             6464..6880
                     /locus_tag="VFB80_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33880.1"
                     /translation="MNTNPAELWALLPLGYLLSVVLETPVLLVGLSRRHSLARRLVAG
                     VWLTACTYPIVVLVLPQLIWRPLGAGGYWPYVTVAEIFAPAAECVLFWLAFWKLAPAE
                     STPHDRRDFLRDMLAIVAANLTSFVAGYWVLDWLGR"
     gene            complement(6970..7152)
                     /locus_tag="VFB80_08675"
     CDS             complement(6970..7152)
                     /locus_tag="VFB80_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HZN33881.1"
                     /translation="MDSEGVYVCDWCGEEIVVPLDLSAGERQEYVEDCPVCCRPNIVV
                     VEVGEDGQMNVWARRE"
     gene            complement(7256..7933)
                     /locus_tag="VFB80_08680"
     CDS             complement(7256..7933)
                     /locus_tag="VFB80_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZN33882.1"
                     /translation="MQRIAVIGLGRFGTSLSRLLAQQRIEVIAVDRNHRLVDEVKDEV
                     TLAVSLDATDEDALSSHEIHRADVCVVAIGENFEASLLAATALKKLGARYVIVRAQTA
                     THAEIFRRLGADEIIQPETEAGRYLARRLANPHLEDVIELSGGYALIELRAPAAFVGK
                     SLEQLGLRNKYRVNLVAVKRQQAIEAGEAAQPAATTYVPSANYTIQADDVLLLVGTDA
                     ALNGLPK"
     gene            complement(8024..9835)
                     /locus_tag="VFB80_08685"
     CDS             complement(8024..9835)
                     /locus_tag="VFB80_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZN33883.1"
                     /translation="MRSPSTTDPTAARDRLALRVLWAERLLTAFTIAVLVLGHGVQRL
                     EAQLGEEIGTLVLAVMALLTAGFAWRAWLNIDWREFLAERRPQTIVHAIWLAGLLLAA
                     FSGVPGLVASGKPLDSVLRWTELLFWARTLVVAIRILRLIAAARTNPAFVFVASFAVL
                     IGTGTLLLMLPAARVQPAGSSDEIGAPPLVALFTATSASCVTGLVVVDTGAYWTRTGQ
                     GIILALIQLGGLGVMTFGAFFALAQRRGFLVREGVFMGQLLEADDRRAVRRLVASILV
                     FTFASELIGAALVATAAPGGTLGERAWFGVFHAISAFCNAGFGLLPKNLEGLGGRWQI
                     WGVVAPLIVIGGLGFEVLRNLAQVILARLAARWPRRDPEQANAPPRLTATTRLVVATT
                     AALLAAGTIAFFILERDVTLRDLPLGERLAASWFQSVTFRTAGFNTVDFTRLAPGTRL
                     IGIVLMFIGASPGSTGGGIKTVVFALLVLVSAATIRGRERVEFAGRTIPDGILKRAAV
                     VAGLAAASLLTSTLLIVIFEQRPELFLDHLFEAASALGTVGLSTVNTATLRPISQCVL
                     VATMFAGRVGPLTLLVALAQPRPQAKYEYPSERVMLG"
     gene            10106..11173
                     /locus_tag="VFB80_08690"
     CDS             10106..11173
                     /locus_tag="VFB80_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZN33884.1"
                     /translation="MFRSPPLAALLAAAILLASAQPAAARSEEETVRLANDVLKEFLD
                     LRVKQIPASLLAEAHGVAIIPNVIKVGFVVGGQHGKGVIVVREPDGSWRAPMFVSLTG
                     GSLGWQIGAQATDFVLVFKTQKSVEGLMRGKFTLGADAAVAAGPVGRRTSAATDTELK
                     AEIYSYSRSRGLFAGISLDGSALQIHEDANAAYYGPPAAGGAPPAVPESALQLVQSIA
                     RLCAATEVVPPGAGAAPAPMEAVPSPAGRPQPPADVEAIQSELVKSMSALSPLLDESW
                     RRYLALPAEVFQRGKAPPPDAVLGVLRRFDAVPQNAQYRPLAERGEFQATHGLLRAYY
                     EALLAGGGPKLSLPPPPGVPR"
     gene            11412..11738
                     /locus_tag="VFB80_08695"
     CDS             11412..11738
                     /locus_tag="VFB80_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33885.1"
                     /translation="MKRILLTVVTASALVLGGWLTGAAQAQYPSYGYGCRPGGVGLAV
                     GYGYPGVNIAYRAPNVALYSPNVAYTSGFAGGYGYRQGCGCQPGLYGAGGYPYSRSRV
                     NFYFGY"
     gene            12158..13486
                     /locus_tag="VFB80_08700"
     CDS             12158..13486
                     /locus_tag="VFB80_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN33886.1"
                     /translation="MSARDNRATLGSMLGSLAWPIILGSGLAVGFYALLLKGVIDQPL
                     AHRYFVGHPVCIVETFFFFVCVAALLQKLFGVWGQYAALSGISLGERQGLQPASAATE
                     MLDKLAGLSAGYRCSYLGRRLTHALESVERNGSAAALESELKHLADVELGRQQDSYAL
                     VRIIVWATPMMGFLGTVIGITQVIAELAHQDMANLQAVMEGLLSGLYVKFDTTALALS
                     YTMLLMFVQFLIDRVEAQVLDAVEVRAADELLGRFELIGVTSDPNVQAMQRMSHGVIK
                     ATEQLVQKQAELWQQSIAAAHDQWQRVSRQTTEQMQTALAGALSQSLAQHAAHLTKSE
                     QASAEQLALRWEQWQTALSHNARLLHAQQQEMVKQGDLMTQAIRVAGDVVQLERALND
                     NLSALSGAKNFEDTVMSLAAAIHLLNARLGKVPDGPHIELKHAQVRARAA"
     gene            13483..>14475
                     /locus_tag="VFB80_08705"
     CDS             13483..>14475
                     /locus_tag="VFB80_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33887.1"
                     /translation="MSRTRRHQVSVSLFPFLAVLICIMGALVILLVLMVARVGAGVKA
                     VASGELAGAGSADDQQLREQLEDAQWQRSMLLQSRSEKTQELADKREELSHLENHIRQ
                     LDAEATRLVEQAKKIDAGQSLREGDLAAARVESAKLKSDIATKRAALEARQAAAAKEP
                     SFALIPYDGPNGTRRRPIYIECTEVGVVVHPEGLLFKADDFSGPLGPGNPLDAALRTI
                     REHLRQTGGKAGEPYPLLVVRPGGVAAYGAARDALQAWDDEFGYELVGDDKRLDFGEP
                     EPALAVALKDAVTAARQRQAMMAAMMPRRFHGESFGGPSPVEGGQMLGAAGSSAP"
BASE COUNT         2365 a         4807 c         4889 g         2414 t
ORIGIN      
        1 ggggcagatc aggatcctgg tcctgcccgt ctgattcata gaatcgagcg ctggaccact
       61 tccagagcct ggcttgtgtc acgagcttgc ggcgcacggg attcaaatgg agatattcga
      121 tgcttcccat cactgctgcc ggcgtctgga ggttgcgatc gtagcccggc ccttcctgcc
      181 agaagcgaaa cgcgtacttt ccgggccgct cgcgcacggt caagcgccga agcaagggac
      241 tatttgcttg ctgaaggtca cctttcgcgt tggcggacac ggggcgcttc acgccggcca
      301 gataagcgtc cagcgccggt tcctccgcca gcggaaagac gagcaagtga acgtgctccg
      361 gcatgaagac aaacgctacg agttggaagc gcaagtgccc gccggcttcg ttgatcgatt
      421 gggagaggga gcgccgccac gcgtcgttgg tcagcaattt catctggtgg aaacacgaga
      481 acgtcagctc gtgcagatcc cccggctcat gatagtgctt gacggtcttg agcttccgcg
      541 gcttcatcgc gaggcctgtt gatcgagact gattcgaaat tcgaccttca tccagaagac
      601 actgacgctg agaattctcg aagatgcaac cgtcgcactt gtcgccgaca ctggcggcac
      661 gccagtgcca cccgaccgct acaactcgcg aatgaacagg ttcgcgaact cgacgacttc
      721 gttgtgatgc tgcaggccga tcgggccgcg cgtcgcgatg ccgggcaatt gggcgtccgt
      781 aacgatttcc ttgccgttga gctcgacgct cacccggtcg cccttcagcg tgatcagaaa
      841 gcggttccat ttgccgagcg gagcgtcggc ccgctctttg ggcgtaaccg cggcgcggac
      901 cgcgggagca agctgcttgt tggtgcggaa ggcggtgacc tcgccgctgc cgacgggata
      961 acagcagatg ttgacctgcg cgtcttcgcg gccgcggagg aggatgccgc tgttgccggc
     1021 gtcatcgacc tcctgcatca cgacctggcc attggcatcg agcttgtcgt cgccgctggg
     1081 caggacgacc ggatacgttt tcttcgtcgg tttggcgggc aatcgccaat cgacgatgag
     1141 ctggaaatcg ccgtactcct tttcggtcca gagatgtttt tccttcggct cggccgcttc
     1201 gctgtggccg tcgtagtgca gcgtccagtt tttcggctgc caatggccgg cgtggccggc
     1261 gggctgccgc cagccggcga gatcgaggcc cgtgtagagc ggaacgaagc cggcggcctc
     1321 ggcagctatt tcgcccggcg gcggcgaggt cgaggggagc tccgcgatcc gcagattgcg
     1381 gaagtggcac tcggagcctt ccgactccag gcagatgtag cccttccgcg gccggcagtt
     1441 gctgccgccc gaaacttcct tgccgttgac ggccagcttc agcacgccgt ccttgcacgt
     1501 gatgcggtag tggttccatt ccggcgacgg cttgcagcgc tgctcgctgg gcaggcagcg
     1561 ctcccagccg ccgggatgcg gccggtcggg cttcatgctc gcgccccaga tgctgaacac
     1621 gtcgccgtgg ctggtgtagt tgtcggcggt gcggccgtcg aggatttgca cctcgatccc
     1681 gcggctgaac ggcgtgccga cggccggcag cggatcgccc cagataaaga cgcccgcgtt
     1741 gccgcccgct ttcaggtgcc gccattccag ctccaggatg aagttttcgt acatccgctc
     1801 gctgcgaatc gtccccgtcg gcacgccggt gctgacgatc aggccgtcct tcaccgagaa
     1861 ggtcgtcggc gcgaggttga cgttgaccca gcccgagagg tccttgccgt taaagagcga
     1921 gcgccagttt tcctgcgcgc tggcagcact ggagagaagg ccgacgaaaa ccgcggtgaa
     1981 gattggcgca agctggcggg acatgatggc cgctccggtg tgacgggctg gtggacgagg
     2041 gtgcgtcccg gaatgtagca ccgccggcgg ccgcgttcta ccgtggccgc ttgcagcggt
     2101 gcgagaggct tcgtagggtg aggccgagga gttcggaatg ccacaggtat tcgacggcaa
     2161 gagcggcagc gccagcgggc ggtttgcgat cgtcgtctcg cggtacaacg aacatatcac
     2221 cgcgaagctg ctggccggtg cggtcgagac gctcggcgcg ggcggcgtgg cggatggcgc
     2281 catcgacgtg gcctgggtgc cgggagcgtg ggagattccg ctcgtggcgg cgaagctggc
     2341 caattcgcag cggtacgtcg ccgtgctctg cctgggcgcg gtgatccgcg gcgaaaccac
     2401 gcacgacgag cacatcaacc ggcaagtcag ccagagcctg ggccagatcg cgctcgaaac
     2461 gggcctgccc gtgctgttcg gcgtgctcac ctgcaacacg ctcgagcagg ccatccaccg
     2521 cgccggcggc gacgccggca acaagggcca ggagtgcgcc gaggccgcgc tggaaatggt
     2581 gcggctgctg gggaagctga gctgagaacc atgagttcca tctgtcccat tcgtcccatc
     2641 gccgttttca tcgggctggc gctctatagc accgccgccg cccaggaacc caagcccgat
     2701 ccattcaccc aggcccgccg cggcctcatc gcgcggcaag tcctggccgc cgccacgcgg
     2761 ctgttcgacg agcggaagtg ggccgaggcg gagaaactgc tcgagggcgc gaccgcgaag
     2821 gattcggcta cgccgaccga tttctacaac ctggcctgcg cccaggcgcg gcaaggcaaa
     2881 acggccgcgg cgctggacag tctcgaagct gccgtcgcca agggctttcg ccaggcgacc
     2941 gaaatggccg ccgatgccga cctggcctcg ctccgcgggg aagagcgctt tcaagcggca
     3001 ctggccaagt gcaaggagtt ggccgcgaaa cctgcggcgc agagcaagct ggagccgctg
     3061 ctggtgggaa aagagggcct tgcgaaagtt ggcccggaga acacggtctg gctgccgcag
     3121 ttcgacctgt ttcaaacggc gttccgcttt gccgataacg acccgcggat cgcggagccc
     3181 gcggtcacgg agcacggcaa gaccggcgag cagattcgcc ggtggcagaa ggagggcacc
     3241 gccgccgggc tgtttggcat cctctacgac aaccgcgacg gcgcccacag ccagctgccg
     3301 ctgagccagt tcccgcagct gaccgccgcc ctgtacggcc cggaggcgaa ggccgacaac
     3361 ctcgacaaag gactggccgc ccgcttcttc ttcaaccggc ccacgttcgg caactcttcg
     3421 accgcgctca ccggcgggcc cttctggcgc agccagacgc ggctcgccca ggtcgattcc
     3481 cgcgcggccg cgctcttgta cgagcagtac gtcaagaacg cactctattt ctatcccgag
     3541 catcgcgact acgatgccgg ccgcaacgga ccggacggtt acggcgacgt ctatcccgcc
     3601 aacacgccgt acgtgatcac cagcgccggc tcgtccggct ccgaccagtc gttcctgcac
     3661 gcggtggcct gcacgctggc cgcctttccg ccgaagacgc aggaactgct catccagcgc
     3721 ggcctgctga tgccgacggt gcagatgatc ctccgccgcg cgtcggtcgg cagcgacgcc
     3781 gagtacctga ccggcaaggc ccatcccgtc gtctttcgcg agcggcagct caatgtcgag
     3841 cggatggtcc tgctagccca cgaaatgcag ccggacgccg tgccgccgat gatccagctg
     3901 caggtggtct cggaggatca gccgcgaaag ggcagcgact atttcgaggc ggccaacctg
     3961 ggcgaacgga tattcgacac gccggcggcg atcgcccgcg tctggcggac gacggcccgc
     4021 gagcggcgga tgatcctcac ggcggcgacg agctacgacg cgaacaatca gccgctgaag
     4081 tttcattggg cggcgctgca agcccggccg ggcagcgtca ccatcaagcc gctcaaggag
     4141 gacggctcgc tggtcgagct gcgcgccctg tggcacgagc ggttcgcgtc cgccgatggc
     4201 gaactggaga ccaaccgcat cgacatcgcg gcgtttgccc acaatggccg ctactattcc
     4261 gcgcccggct ttgtgacgtt cttctgcctc gacgacgagg agcggaaata cgacgagcag
     4321 ggccgcatcg cctcggtcgc ctatcgctca ccggccaagg ggggcaacta tgccgacccg
     4381 atgacgcaca ccctgcgaga ttggcgcgac gagtatcagt acgacgccgc gggccggctg
     4441 accggctgga cccgcgtccg cggcgagcag cgggaatatt tcaccgccca cgggcatctg
     4501 atctcgcaga aggacgacaa gggccgcgca cgcacggccc gcaaggtcag ctacgtcccc
     4561 aaggccgctc agccgggtga attggcgacc ctggaggtag tggccgacgc ggaactgctg
     4621 acttatgcgt acgacggcga agacgaccgc gtgggccaca tcgcggacca gttgccggat
     4681 gcgaaaacca ggtaggcgcc caggtcgtct ggtactgcaa gcttgcgtcg gtgcgggccg
     4741 gcgctgaaaa tgcgcctgaa ttcagaggtt caatctttct gtggagtttc gcaatgaacg
     4801 gactggtcac gttgacatcg gcggcattgc tcctggcgtg gaatgcgatc gccccgggga
     4861 atgtcgctcc gcctcccaag aagcccgagc gggtggttgc cgaagtgaag atcgagcgcg
     4921 gcgcgatcaa gggcgaaggc cgcgcggtgc aggccaagat catcattccc aagggcctcg
     4981 tgcacggcgg cgctgccggt gcagttcccg tcgtcccggc gcccggggct gctccagcgc
     5041 ctcgggcagt gcccgcgccc aacgctcgcg accaggctgg gctgcccttc ggtacgatca
     5101 tcgcgggagt ctgtatgtcg ctggccgcgg tcagcctcgt gttcgtcgtc cgcggaaacc
     5161 gcggtgcgaa gacaacggca ctggcaatcg tggccggcgc gctggtcctg ggcgcgttca
     5221 gcgcggccta tgccgacttg attcccggcc gccccaatgg tcctgtgccg gtgccggcag
     5281 aaatcgtcat cgagctggtc gatggtggcg acacggtgac gctccagctt gcccgctagc
     5341 gccgcgcgat cgctcggtag ccgaattcgc cagaattcgg tctttccagg tttctcgtcc
     5401 gaactctggc gaattcggct acacccccgc atggctaccg caactctcga tgacgttgct
     5461 ccgccgcgag ccgaatcgcc ggcccgctcg gcgctcgtct ttgcggcggc ggcgacggcc
     5521 gtgattgcgg cgtcgtgcct gctggcgggg ttcgcgccgc tggggttctc gatcgtcacg
     5581 gtgttcctgt tcgccgggcc gcacaattgg ctcgaggccc ggtacatgct cacgcggatg
     5641 cccgctcgct ggggtccgct gacgctgtac ttcacaaccg gcatcgccgg agtgctgctc
     5701 ttggcagctg ggctagccgc cgtccgctgg ctgggcgagt ggctgcggct ggactccgag
     5761 atgtggctcg cgctctacgc aggatggaac tcggtcttcg tcgcctgggt cgctgcgctg
     5821 gccctgatgc gcagccggca gaacccgcgg cggaattggc tctggatcgt accgggggcg
     5881 tttgtgctga tcgcactcaa ctggctgtgg ccgatgacct ggagcctggc cctggtctat
     5941 ctgcatccgc tcgtggccct gtggtttctc gaccgcgaga tcggcaagat gcagccggcg
     6001 tggcgcacgg cctatcgcgg ctgcctgctg ctcgtgccgg cggcgctggg cgtactctgg
     6061 tggaacctgg cctcggcccc gaacttgccc ggcaacgact ggctgcagca gcagatcacg
     6121 cagcacgccg gcgcgggcat cctgagcggc gtctcgacgc atttgcttgt ctccacgcac
     6181 acgtttttgg agatgctgca ctacggcgtg tggatcgtgg cgattccgct ggtcagcgtg
     6241 cggatgacgc cctggcagct cgacaacgtg ccgctggccc gccggtccaa tgcctggcgg
     6301 gcggcgatcc tggccgccgt cgccgcgggc gtgatcgtga tgctcgtcct gtggggcggc
     6361 tttgtcgcga attatccgct gacccgcgac atctacttca ccgtggcgat gctgcacgtc
     6421 ttggccgagg ttccgttcct gctgcggctg ttgtgagcgg cggatgaaca ccaatcccgc
     6481 cgagttgtgg gcgctgttgc cactgggcta tctgctgagt gtcgtgctgg aaacgccggt
     6541 gctgctggtg gggctgtccc ggcggcactc gctggcccgc cggctcgtag ccggagtctg
     6601 gctgaccgcc tgcacgtacc cgatcgtcgt actggtgctg ccgcagctga tctggcggcc
     6661 gctgggcgcg ggcggctatt ggccgtacgt gacggtcgcc gagatctttg cccctgccgc
     6721 cgagtgcgtg ctgttctggc tggcgttctg gaagctcgcg ccggcggaat cgactccgca
     6781 tgaccgccgc gatttcctcc gcgacatgct ggcgattgtg gcggcgaacc tgacgtcgtt
     6841 tgtggccggg tattgggtgc tggactggtt gggtcggtag gtcagcggtc ggtaggtcag
     6901 tcagtcggag gcggtcagtc gacaatcaac cgactaaccg accaccgacc gaccgaccga
     6961 ccaccgcccc tactcgcgcc tcgcccaaac attcatctgg ccgtcctcgc cgacctcgac
     7021 gacgacgatg ttcggccggc agcagacggg gcaatcttcg acatactcct gccgctcgcc
     7081 cgccgacaga tccagcggaa cgacgatctc ctcgccgcac cagtcgcaga catacacgcc
     7141 ttcgctgtcc atgaaacacc actcccactt gggcagtcag tcagtcagtc ggtggtcggt
     7201 tcgtcggtaa ggtcccaaac cgactaactg actaacggac caaccgatcg accgactact
     7261 tcggcaaccc attcagcgcc gcatcggtcc cgaccagcaa cagcacgtcg tccgcctgaa
     7321 ttgtatagtt ggcgctgggc acgtaagtcg tcgccgccgg ctgcgcagcc tcgcccgctt
     7381 cgatcgcctg ctggcgtttg acggcaacca ggttcacgcg gtacttgttc cgcaggccga
     7441 gctgctcgag cgacttgccg acgaacgcgg ccggcgcacg cagctcgatc agcgcgtagc
     7501 cgcccgacag ctcgatcacg tcctccaggt gcggattggc caggcggcgg gccagatagc
     7561 ggcccgcttc ggtctcgggc tgaatgatct catccgcgcc caggcggcgg aagatttcgg
     7621 catgcgtcgc cgtctgggcc cggacgatga cgtagcgcgc tccgagcttc ttgagcgccg
     7681 tcgcggcgag cagcgaggcc tcgaaattct cgccgatggc cacgacgcag acgtcggcgc
     7741 ggtggatctc gtggctcgag agcgcgtctt cgtcggtcgc gtccagggag acggccagcg
     7801 tcacctcgtc cttgacttcg tccacgaggc ggtggttgcg atcgacggcg atcacctcga
     7861 tccgctgctg ggccagcagc cgcgacaggc tcgtgccaaa ccgtcccagg ccgatcaccg
     7921 cgatgcgttg cactgttttt ttccgtagcc gaattcgcca gaattcggat cttgtttcac
     7981 cgaccgaact ctggcgagtt cggctaccac gactcgctcc tcgctagccc agcatcaccc
     8041 gctcgctggg atattcgtat ttggcctgcg gccgcggctg ggccagggcg acgagcagcg
     8101 tcagcggccc gacgcggccg gcgaacatcg tggccacgag cacgcactgc gaaatcggcc
     8161 gcagggtcgc cgtgttgacg gtcgagagcc cgaccgtgcc caaggcgctg gccgcttcga
     8221 acaggtgatc gagaaacaac tccgggcgct gctcaaagat cacgatgagc agcgtggaag
     8281 tcaacaggct ggcggcggcc agcccggcca cgacggccgc ccgcttcagg atgccgtcgg
     8341 gaatcgtgcg cccggcgaac tcgacccgct cccggccgcg gatcgtcgcc gccgaaacga
     8401 ggacgagcag tgcgaagacc acggtcttga tgccgccgcc cgtcgagccc ggtgaagccc
     8461 cgatgaacat caggacgatc ccgatcagcc gcgtgcccgg ggcgagccgc gtgaaatcga
     8521 cggtattgaa cccggcggtg cggaacgtga ccgactggaa ccagctcgcc gccagccgct
     8581 cgcccagcgg caggtcgcgg agagtcacgt cgcgctccag gatgaaaaag gcgatggtgc
     8641 cggccgccag caaggctgcg gtcgtcgcaa ccacgagtcg cgtcgtcgcc gtcagccgcg
     8701 gcggcgcatt cgcctgctct ggatcgcgcc ggggccagcg cgcggccagc cgcgcaagga
     8761 tcacctgcgc gagattccgc agcacttcga accccaagcc gccgatgacg atcagcggag
     8821 cgacgacgcc ccagatttgc caacgcccgc cgagcccttc caggtttttc ggcaagagcc
     8881 caaacccggc gttgcagaac gcgctgatcg cgtggaacac accgaaccag gcccgttcgc
     8941 cgagcgttcc gccgggcgcg gccgtcgcga ccagcgccgc gccgatcagc tcgctggcga
     9001 aggtgaagac caggatcgat gcgaccaggc ggcggacagc ccggcgatcg tcggcttcga
     9061 gcagctggcc catgaagacc ccttcgcgga ccaggaagcc gcgccgctgg gccagcgcaa
     9121 agaaggcgcc aaacgtcatg acgcccaggc cgccaagttg aatcagagcc aggatgatcc
     9181 cctgccccgt ccgcgtccaa taggcgcccg tatcgacgac gaccagcccc gtcacgcagc
     9241 tcgcgctggt cgccgtgaac agggccacca gcggcggcgc gccgatttca tccgagcttc
     9301 ccgccggctg cacgcgggcg gccggcagca tgagcagcag cgttcccgtg ccaatcagca
     9361 ccgcgaacga ggcgacaaag acgaaggcgg ggttggtgcg ggccgcggcg atcagccgca
     9421 ggatgcggat tgccacgacg agcgttcgcg cccagaacag gagctccgtc cagcggagga
     9481 cggagtcgag cggcttgccg ctggcgacca gtcccggcac tccggagaaa gccgccagca
     9541 gcaggcccgc cagccagatc gcatggacga tcgtctgcgg ccggcgctcg gccaggaact
     9601 cgcgccaatc gatgttcagc caggcccgcc acgcgaagcc cgcggtgagc agagccatca
     9661 ccgccagcac gagagtgccg atctcctcgc ccagctgcgc ttccaagcgc tgtacgccgt
     9721 ggccgagcac caggaccgcg atggtgaagg ccgtcagcaa tcgctcggcc cacagcacgc
     9781 gcagcgccaa gcgatcgcgg gccgcggtgg ggtccgtagt cgatggggat cgcatgcggg
     9841 cagaacgagt gtgatagcag tcgggtgcca gcggctattg tgcaaagcgg cgatgcgact
     9901 gcaacggtgt gtaactttgg gcaaagcgcc gccaccggct gtggggcgcg atgggggcgc
     9961 ggtaagtctg cacctggcag caggttagat gcggacgcgc tgcctgcgtg ggccgggctc
    10021 gtgcgctccg gcgcggcgat tgcagttgtc ccaggccgcg gcctcgcgta ggtttccgcc
    10081 gcacgccagc cgaaaggagt cgcctgtgtt tcgctcacca ccgctcgccg ctttgctcgc
    10141 ggcggcaatt ctgctggcca gcgcccagcc cgcagccgcc cgctcggaag aggagacggt
    10201 ccgcctggcc aacgacgtcc tcaaggaatt cctcgacctg cgggtgaagc agattccggc
    10261 gtcactgctg gcggaagctc acggcgtggc gattattccc aacgtcatca aggtcggctt
    10321 cgtcgtcggc ggccagcatg gcaagggcgt gatcgtcgtc cgcgaacccg acggctcgtg
    10381 gcgcgcgccg atgttcgtgt cgctcaccgg cggcagcctc ggctggcaaa tcggcgcgca
    10441 ggcgaccgac ttcgtgctgg tgttcaagac ccagaagagc gtcgaggggt tgatgcgcgg
    10501 caagttcacg ctcggggccg acgccgccgt cgccgccggg cctgtcggcc ggaggaccag
    10561 cgccgcgacc gacaccgaat tgaaggcgga gatttactcg tattcccgca gccgcgggct
    10621 gtttgccggc atttcactcg acggctcggc cctgcagatt cacgaggacg ccaatgcggc
    10681 gtattacgga ccgccggcag ccggcggcgc gccaccggcg gtgccggaat cggccctgca
    10741 attggtccag tccatcgcga ggctttgcgc ggcgacggag gtggtgccac caggagccgg
    10801 cgccgcgccc gcgccgatgg aagccgtgcc gagtccggcc ggccggccgc agccaccggc
    10861 cgacgtggag gcgattcaaa gcgagctcgt caagtcgatg tcggcgctga gcccgctctt
    10921 ggacgaatcc tggcggcgct acctggcgct gcccgcggaa gtgtttcagc gcggcaaagc
    10981 gccgccgccc gatgccgtgt tgggggtcct gcggagattc gacgcggtgc cgcagaacgc
    11041 gcagtatcgc ccgctggcgg aacgcggcga attccaggcg acgcacggcc tgctgcgggc
    11101 ctactacgaa gcgctgctgg cgggcggagg ccccaagctc tcgttgccgc cgccgccggg
    11161 cgtgccacgc taggaaatcc gcgcagcact cttgccgtca gactgatgac gctgcgccat
    11221 cactctgaga tcaattcggg tctggcggat ttgcccgccc gagcgcttct agtgaatcgc
    11281 atcttcgttc gcagccatca gttgcggtgc tgcgactgct gctgcggcac gcgtggtgct
    11341 tgcacggtgg catccgtggt cgcggtgtgc gactacgatc cctcaccacg ttggcacgaa
    11401 aggacactct tatgaagcgc atcttgctga ccgttgtgac ggccagcgcg ctggttctcg
    11461 gcggatggtt gaccggcgcc gcccaggctc aatatcccag ttacggctac ggctgtcgtc
    11521 cgggcggcgt cggactcgcg gtcgggtatg gatatccggg cgtgaacatt gcgtatcgcg
    11581 cgccgaacgt tgccctgtac tcgccgaacg tcgcttacac gtcgggcttt gccgggggct
    11641 acggctatcg ccagggttgc ggttgtcagc cgggactcta tggggcaggc ggctatccgt
    11701 attcgcggtc gcgcgtgaac ttttactttg ggtactaggt tcggacggga cgtggagagg
    11761 ttgacagtcg gacgggccag gactgtcaag aaatgagaaa gtccgggcga gccttggtgg
    11821 ctcgcccgga cgatcgtccc ccccgggatt tgtcgccgtg ttcgtcttct tggttgcccc
    11881 ctcgcccgcg gcgacgctgt tcttcatcgg ccggccttgg cgcgacactt tggcaatctt
    11941 tgccgattgt gccagtcgta acgcggttgg tcttggcgaa gcagaccggt ccgtgtatgg
    12001 acgcggtttg gcgcgccttg ctgccagctt cgctagcgat ctgaaatgac cgaattgcct
    12061 cgttaagctg tagcgatcag gacgatatta gcggcaagcg aaactctgcg gccgccattg
    12121 cgcgggccgc aggccgccgt gagaaaggat gctccccgtg tccgcacgcg acaaccgcgc
    12181 caccctgggt tcgatgctcg gcagcctggc ctggccgatc attttgggca gcggcctggc
    12241 ggtcggcttc tacgccctgc tgctcaaagg cgtcatcgat caaccgctcg cgcatcgcta
    12301 tttcgtcggc cacccggttt gcatcgtcga gacgttcttc ttcttcgttt gcgtcgcggc
    12361 cctgctgcaa aagctgttcg gcgtttgggg ccaatacgcg gccctttcgg gcatctcgct
    12421 gggcgagcgg cagggattgc agccggccag cgccgcgacc gagatgctcg acaagctggc
    12481 gggactctcg gccggctacc gctgctcgta cctcggccgc cggctgacgc acgcgctgga
    12541 atcggtcgag cggaacggct cggccgcggc cctggagtca gagctcaagc acctggccga
    12601 cgtggagctc ggccgccagc aggacagcta cgccctggtg cggatcatcg tctgggcgac
    12661 gcccatgatg ggctttttgg gcacggtcat cggcatcacg caggtcatcg ccgagctggc
    12721 ccatcaggac atggccaacc tgcaagcggt gatggaaggg ctgctcagcg gcctgtatgt
    12781 caagttcgac acgaccgcgc tggccctcag ctacacgatg ctgctgatgt tcgtgcagtt
    12841 cctcatcgac cgggtggagg cccaggtcct ggacgcggtc gaagtgcggg ccgccgacga
    12901 gctgctgggg cggttcgagc tgatcggcgt gaccagcgat ccgaacgtgc aagcgatgca
    12961 gcggatgagc cacggcgtca tcaaggcgac cgagcagctc gtccagaagc aggccgagct
    13021 gtggcaacag tcgatcgccg ccgctcacga ccagtggcag cgggtttcgc ggcaaacgac
    13081 cgagcagatg cagacggcgc tggccggggc gctcagccaa tcgctggccc agcacgcggc
    13141 acacctgacg aagtccgaac aggcctccgc cgagcagctc gccctccgct gggagcagtg
    13201 gcagacggcc ctctcgcaca acgcccgcct gctgcacgcc cagcagcagg agatggtgaa
    13261 gcagggcgac ctgatgacgc aggcgatccg cgtggccggc gacgtcgtgc agctcgagcg
    13321 ggcgctcaac gacaacctct cggcactctc cggggcgaag aactttgaag acaccgtgat
    13381 gagcctcgcc gcggcgatcc acctgctcaa cgcgcggctg ggcaaggttc ccgacgggcc
    13441 ccacatcgag ctcaagcacg cccaggtgag ggcccgcgct gcatgagtcg cacccgccgg
    13501 caccaggtca gcgtctcgct gttcccgttc ctcgcggtgc tgatctgcat catgggggcg
    13561 ctcgtcatcc tgctcgtgct gatggtggcc cgcgtcggcg caggcgtgaa ggccgtggcc
    13621 agcggagaac tagctggggc cggcagcgcc gacgaccagc agctccgcga gcagctggaa
    13681 gacgcccagt ggcagcggtc catgctgctg caaagccgca gcgaaaagac gcaggagctg
    13741 gccgacaagc gcgaggagct ctcgcacctg gagaaccaca tccggcagct cgacgcagag
    13801 gctacgcggc tggtcgagca ggccaaaaaa atcgatgccg gccagtcgct gcgcgagggt
    13861 gacctggccg ccgcccgggt cgagtccgcg aagctcaaat ccgacattgc gacgaagcgg
    13921 gcggcgctcg aagcccgcca ggccgccgcg gcgaaggagc cctcattcgc cctgattcct
    13981 tacgacggcc ccaacggcac gcggcggcgg ccgatttaca tcgagtgcac cgaggtcggc
    14041 gtcgtcgtgc atccggaagg gctgctcttc aaggccgacg actttagcgg ccccctggga
    14101 ccgggcaatc cgctcgatgc cgccctgcgg acgattcgcg agcacctgcg gcaaacgggc
    14161 ggcaaagccg gcgagcccta tccgctgctc gtggtgcggc ccggcggcgt ggcggcgtac
    14221 ggcgcggccc gcgatgccct gcaggcctgg gacgacgaat tcggctacga gctggtcggc
    14281 gacgacaagc ggctggactt cggcgagccg gagccggcgc tggctgtggc gctgaaggac
    14341 gccgtcacgg ccgcccgcca gcggcaggcg atgatggccg cgatgatgcc gcggcggttc
    14401 cacggcgaat cgttcggcgg cccatcaccc gtcgagggcg gccagatgct gggcgcggcc
    14461 ggttcctccg cgccg
//
LOCUS       DASRJB010000324         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1784793, whole genome shotgun sequence.
ACCESSION   DASRJB010000324 DASRJB010000000
VERSION     DASRJB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784793"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFB80_08710"
     CDS             complement(<1..642)
                     /locus_tag="VFB80_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN33888.1"
                     /translation="MKRLLPFVSFVYFVVPLVASAQQPSFVAKEINAKSEFPACAVID
                     VNHDGKLDIVSGGFWYEAPQWKRHFLREVEMIRGRFDDYSNLEIDVNADGWTDILSCN
                     YRSQSLFWMEHPGEKIKSDPETPWIKHLIDTPGPMETGRLFDIDADGDLDVLPNGTTF
                     AAWYELTYEVADGKKQPRFIRRDLPKEIAGHGVGFGDINGDGRGDIVGPRGWLE"
     gene            complement(639..1586)
                     /locus_tag="VFB80_08715"
     CDS             complement(639..1586)
                     /locus_tag="VFB80_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN33889.1"
                     /translation="MACRLASTLLTALCFASAVTAAEPAKKVLYIGIDGTRFDAIEKA
                     ETPNLDALVKEGIYSPTCLILGDRYRKNDTVSGPGWSSILTGVWADKHGVHNNTFVGM
                     NYKEFPHFFARLKEVRPDAKTASLVTWEPIHKHITSAADVSINYEQKEHGVLDYDRYD
                     TQATEETVKQLTEQNPDAVFLYIGQVDVAGHAKGFHPSVPDYIAAIERADKLVGRAVA
                     AVKGRKTYAGEDWLILVTSDHGGKGTGHGSGHNTPEILNSFLIVSGTSAIRGQFAEQV
                     YLVDAPVTALAHLGVKAKEEWKLDGRPVGLKNNTSAKSP"
     gene            complement(1688..>2404)
                     /locus_tag="VFB80_08720"
     CDS             complement(1688..>2404)
                     /locus_tag="VFB80_08720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN33890.1"
                     /translation="EENKCDEAVLYGVAGQAAYSLDDYETAEKYLAIADKAGKLDREG
                     QNMFKELPEQKKAWAAEQAIRMKEAAAGDLPRVKLETSKGPIVIELLENEAPQAVGNF
                     VSLVEKKFYDGLVFHRVLPNFMAQGGDPKGDGTGGPGYNIYCECGQENARKHFRGTLS
                     MAHAGKDTGGSQFFLTFRPTTYLNGRHTVFGRVIEGFDVLAKLQRVNPQAANQPAPDK
                     IVKAEVVRKREHKYEPKKVE"
BASE COUNT          356 a          747 c          794 g          507 t
ORIGIN      
        1 ttccagccag cctcgcgggc cgacgatgtc gccgcgtccg tcgccgttga tgtcgccaaa
       61 gcccacgccg tggccggcaa tctccttggg caaatcgcgg cggataaacc gcggctgctt
      121 cttgccgtcg gccacttcat aagtcagctc gtaccaggcg gcgaacgtcg tcccgttggg
      181 cagcacgtcc aggtcgccgt ccgcatcgat gtcgaacagc cggccggtct ccatcgggcc
      241 gggtgtgtcg atcaggtgtt tgatccaggg agtctcgggg tcgctcttga tcttctcgcc
      301 gggatgctcc atccagaaca gcgactggct gcggtagttg cagctcagga tgtcggtcca
      361 gccgtcggcg ttgacgtcga tctccaggtt cgaatagtcg tcgaaccggc cgcgaatcat
      421 ttcgacctcg cggaggaagt gccgcttcca ctgcggagct tcgtaccaga acccgccgct
      481 gacgatgtcg agcttgccgt cgtggttcac gtcgatcacc gcgcaggccg ggaactccga
      541 cttggcgttg atctccttcg cgacgaagct cggctgctgc gccgaggcga cgaggggaac
      601 cacgaaatac acgaacgaca cgaaagggag gaggcgtttc atggggattt cgcgcttgtg
      661 ttgtttttga ggccgacggg cctgccgtcg agcttccatt cttccttggc tttgacgccg
      721 aggtgggcca gggcggtgac gggggcatcg acgagataga cttgctcggc aaactggccg
      781 cggatcgcgg aggtgccgct gacgatcagg aagctgttca ggatctcggg cgtgttgtgg
      841 ccgctgccgt ggccggtgcc tttgccgccg tggtcgctgg tgacgaggat cagccagtcc
      901 tcgccggcgt acgtcttgcg gcccttcacc gcggcgaccg cgcggcccac gagcttgtcg
      961 gcccgctcga tggcggcgat gtaatctggc acgctcggat gaaaaccctt ggcgtggccg
     1021 gcgacgtcaa cctggccgat gtagaggaag accgcatcgg gattctgctc cgtcagttgc
     1081 ttgacggttt cctccgtggc ctgcgtgtcg tagcggtcgt agtcgagaac gccgtgttcc
     1141 ttctgctcgt agttaatcga cacgtcggcg gcggacgtga tgtgcttgtg gatcggctcc
     1201 caggtgacca gcgacgcggt ctttgcgtct ggccggactt ccttcagccg ggcgaagaag
     1261 tgcggaaact ccttgtagtt catgccgacg aacgtgttgt tatgcacgcc gtgcttgtcg
     1321 gcccagacgc cggtgagaat gctcgaccag ccggggccgc tgaccgtgtc gttcttgcgg
     1381 tagcggtcgc ccaggatcag gcaggtcggc gagtagatgc cctccttcac cagcgcatcg
     1441 aggtttggcg tctcggcctt ttcaatcgcg tcgaaccgcg tgccgtcgat gccgatatag
     1501 aggactttct tggcgggttc ggcagcggta acagcgctgg cgaagcagag agcggtcagc
     1561 agcgtcgaag ccaagcggca ggccatgatg gatctccagt ggccgcagta gtttaacccg
     1621 cagccggagg cgacggcgtc cgctgcggcc acctgcgcct ccggctgcgg gttaaacgag
     1681 ccgctggcta ctcgactttc ttcggctcgt acttgtgctc gcgcttgcgg acgacttcgg
     1741 ccttgacgat cttgtccggc gcgggctgat tggcggcctg cgggttgacc cgctgcagct
     1801 tcgccagcac atcgaaaccc tcgattacgc ggccgaagac cgtgtggcgg ccgttgagat
     1861 aggtcgtggg gcggaaggtc aggaagaact gcgagccgcc ggtgtctttg ccggcgtggg
     1921 ccatgctcag tgtgccgcga aagtgtttgc gggcgttttc ctgcccgcat tcgcagtaga
     1981 tgttgtagcc cggcccgccg gtgccgtcgc ccttcgggtc gccgccctgg gccatgaagt
     2041 tcggcagcac gcggtgaaac accaggccgt cgtagaattt cttctccacg aggctcacga
     2101 aattgccgac tgcctgcggg gcctcgtttt ccaggagctc gatcacgatc gggcccttgc
     2161 tggtttcgag cttcacccgc ggcaggtcgc ccgcggcggc ctccttcatg cggatcgcct
     2221 gctcggcggc ccaggccttc ttctgctcgg gcagctcctt gaacatgttc tggccctcgc
     2281 ggtccagctt gcccgccttg tcggcgatcg ccaggtattt ctcggccgtt tcataatcgt
     2341 cgaggctgta ggccgcctgg ccggcgacgc cgtacagcac ggcctcgtcg cacttgtttt
     2401 cctc
//
LOCUS       DASRJB010000325         8671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2059412, whole genome shotgun sequence.
ACCESSION   DASRJB010000325 DASRJB010000000
VERSION     DASRJB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059412"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFB80_08725"
     CDS             <1..630
                     /locus_tag="VFB80_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZN33891.1"
                     /translation="SAVIAENQAEGVIPLTANGGAGIGNWKIIVTGRTGSRGQRGGGG
                     EDDSFRCSSGFADLAVSDQYYKLAFNKTAAEQGQEAEVAVKVEKLKDFEGTAKAELVG
                     LPANTSTQPIEFSKDTTELVFKVNVTKDAKPGRYPSLVCVTKFAVAGETVTHTLGTGE
                     LRVDAPLPPKPNATAAAKPAAGQPAAAAAPKRLSRLEQLRLEKEQEEKK"
     gene            705..3116
                     /locus_tag="VFB80_08730"
     CDS             705..3116
                     /locus_tag="VFB80_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33892.1"
                     /translation="MKLIAIFASLTLATTALADGQITSVSVYPPDIRLNTKADLQRFI
                     VVATRSDGVTLDVTGQAAVKLVDGKLCKLDKNLLTPLADGQTTLEIEYQGFKSAATVE
                     VKDAAADRPISFQLDVMPLFMRGGCNTGSCHGAARGKDGFRLSLFGFDPQGDYYRITR
                     EIGVRRINLASPAESLMVEKTTGAVPHTGGKRFELESEYGRTLLRWLSEGAANDPKPT
                     PAVIGVDLFPPSAVLEGEGATQQFIARARYADGTDRDVTSLATFLTNNDNSAPISVDG
                     LLTAGARGEAFVMARFETHTVGNQVLVLPKGLMYAAPPVSGNYIDELVGAKLNKIRIL
                     PSEVCSDEEFLRRATIDIAGQLPTPEEYQAFMADASKDKRAQLVNRLLERKEFSEIWA
                     MKWAELLMIKSSNQVSAKSAFLYYSWLTDKIAGNVPLDQMVAELLSATGGTFKSPATN
                     YYQIERDTLKTAENVAQVFFGIRTQCAQCHNHPFDRWTMDDYYSFAAFFAQIGRKQSE
                     DYREIIVFNSGGGEVRHPVGGRNMPPKFLGGAAPDTAGKDRREIVAQWIASADNPYFS
                     TSVANRVWAHFFGKGIVEPVDDIRVSNPPSNPELFQALGKKLVEYKYDFRQLVRDICS
                     SNAYQRTATRNESNATDERNFAHANVRRIPAEQLLDCVSQVTGASDKFQGLPLGARAV
                     EIANGSTSNYFLTTFGRAPRETVCACEAKTEPTLSQALHMLNGNTIQGKIQQGGVVKK
                     LLDAGQTPPQVIESLYIRALARKPTPEEAERLLTVVGQSANPQQGLEDVFWAVLNSRE
                     FIFNH"
     gene            3218..5752
                     /locus_tag="VFB80_08735"
     CDS             3218..5752
                     /locus_tag="VFB80_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN33893.1"
                     /translation="MRLFLASLFAAAIGTTTAATAQESKPAAPAGPRITYDDHVRPVL
                     REHCFTCHSADKQESGLALDTYAKAMAGGSSGEIVIAGDLGSSRLWALVSHAEDPKMP
                     PKQDKLPAAKLELISKWIEQGAPENAGSKVAIKKNPLAVAAVGKSGKPSGPAAMPEGL
                     FKQPVVVTEKPGQITALASSPWAPLVAVAGQKQVVLYQSDSGQLSGVLPFPEGIPHVL
                     RFSRNGSVLLAAGGRGGHSGCVALYDVKSGRRIAKIGDELDAVLAADINSSHTLVALG
                     GPNRVVRIFSVQTGELLHEIRKHTDWVYCVEFSPDGVLLATGDRSGGLFVWEADTARE
                     YLNLRGHNGAICDVSWRLDSNVLASAGEDTTIKLWELNDGNAIKSWNAHGGGSFCVEF
                     THDGRLVSAGRDNTVKTWNGDGAAQKSFPAFPEAALRCTFTHDGNRVVGGDWLGHVNV
                     WDAGQATLVYSLAPNPPTLAKRVELAKADLTAKQAAVAAVNAELAAVTKVATDKAAAL
                     KAATDKQAAAAATAQKAEADRLAAVKAAETAAAAVKPATDAAAAAQAALTAPTAEKAA
                     ADKTVADKTAAAKTAADKAAVDKAGADKLAAEKNPEAAKALETAAASQVVAAQSEVER
                     AQAAVVAAQKAEALTKAANSAAAAATAAEKARADAAQAAKQVETMTSAAKAAADAVPS
                     AKAEVDKATKEKSDADAQVAAKKPGADAAQTAVKAAEAALQQAETDLKNFSETSAKLA
                     AAAKAAADQVAAKQAAVTAAESETKATADAAAQKAAAAKKLAEQLAALQAELAKAQSD
                     QKAADDAAAAKAKAVADAQAALAAAKAAADAVAAEQKAFADAYGPK"
     gene            complement(5815..6045)
                     /locus_tag="VFB80_08740"
     CDS             complement(5815..6045)
                     /locus_tag="VFB80_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33894.1"
                     /translation="MERCKNVFEAILKYGHDEDWLPDSKSNFDATDAPAGSQEKIEIL
                     RRRLELGLPLWHDSDRYDYSGLVGAIRPRDSM"
     gene            6711..7130
                     /locus_tag="VFB80_08745"
     CDS             6711..7130
                     /locus_tag="VFB80_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZN33895.1"
                     /translation="MSWLQKHLALTPRSRGCHLITREVEQALPEIASLRAGILHVFIQ
                     HTSASLTINENADRDVRVDLESSLNVLAPEDFPYVHTLEGPDDMPAHLKASLLGSSVT
                     IPVGGGKLLLGTWQGIYLCEHRNSGGSRNLVLTLWGD"
     gene            complement(7127..8188)
                     /locus_tag="VFB80_08750"
     CDS             complement(7127..8188)
                     /locus_tag="VFB80_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HZN33896.1"
                     /translation="MSWQSIRGHDAIAERFRQALARGRLVSTFLFVGPAGIGKRAFAL
                     QLAQGLLCERAAEERLDPCGQCRSCHQVLAGSHPDVELIGKPADKSFIPVELLIGDKE
                     HRMQEGLLYNISRKPYSGRHKVAILDDADYLNKEGANCLLKTLEEPPPKSLLILLGTS
                     EQRQLPTIRSRCQIVRFQPLAESDVAELLLERRLCDDPATARRAAARSGGSLQLAQLW
                     CDERMAEFRGQLLGLLSRGDFDPLAAAKLIGQFADDAGKESALKRERLRLAVSLAEEF
                     YRAALLSITAGRTPADAELAAAVKEAASWLPGDEAAAACLDFCLDAYGYIDANVNQAV
                     FIEWWLDELGSAARGKVPV"
     gene            complement(8223..>8671)
                     /locus_tag="VFB80_08755"
     CDS             complement(8223..>8671)
                     /locus_tag="VFB80_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33897.1"
                     /translation="HFSRQAALESAAQRATEGWLVLVQAGKLEEAHQLRMAPQARIAD
                     AEAVKDYYEKNAEARQELQSFKAQKGIADLVAAGPQGSVRYEGVTVTYNEGFSDQIQL
                     RYVLSRPAERGGDLPLWISLARRVEEKSHHAHWEVTAITTTPPTPQ"
BASE COUNT         1633 a         2873 c         2846 g         1319 t
ORIGIN      
        1 agcgccgtga tcgcagagaa ccaggctgaa ggcgtcattc cgctcaccgc caacggcggc
       61 gctggcatcg gcaattggaa gatcatcgtc accggccgca ccggcagccg ggggcagcgg
      121 ggcggcggcg gcgaagacga ctcgttccgc tgctcgagcg gcttcgccga cctggcggtc
      181 agcgaccagt actacaagct ggccttcaac aagacggcag ccgagcaggg ccaagaggcc
      241 gaggtcgccg tcaaggtcga gaagctgaag gacttcgaag gcacggcgaa ggccgagctc
      301 gtcggcctgc cggcgaacac cagcacgcag ccgatcgagt tcagcaagga cacgaccgag
      361 ctggtcttca aggtgaatgt caccaaggac gccaaacccg gccggtatcc gtcgctggtc
      421 tgcgtgacga aattcgccgt cgccggcgag accgtgacgc acacgctggg cacgggcgaa
      481 ttgcgggtcg atgccccgct gccgcccaag cccaacgcga ccgccgccgc caagccggcc
      541 gcgggccagc ccgcagccgc cgccgctccc aagcggctgt cgcggctgga gcaattgcgg
      601 ctcgagaagg aacaagaaga gaagaagtag tcgtagcata agcgtcctcg cttgtgccag
      661 atggatcagt gagcaagcga gacgcttgct ctacggagaa caccatgaaa ctcattgcga
      721 tatttgcctc gctgaccctc gccactactg ccctcgccga cggccagatc accagcgtca
      781 gcgtctatcc gcccgacatc cgcctgaaca ccaaggccga cctgcagcgg ttcatcgtcg
      841 tcgccacgcg cagcgatggc gtgacgctcg acgtcaccgg ccaggcggcc gtgaagctgg
      901 tcgatggcaa gctctgcaag ctggataaga acctgctcac tccgctggcc gatggccaga
      961 cgacgctgga gatcgagtac cagggcttca aatcggccgc gacggtcgag gtcaaggacg
     1021 cggccgccga tcgcccgatc agcttccagc tcgacgtgat gccgctgttc atgcgcggcg
     1081 gctgcaacac gggcagctgc cacggcgcgg cccgtggcaa ggacggcttc cgcctgtcgc
     1141 tgtttggctt cgacccgcag ggggactatt accgcattac gcgcgagatc ggcgtgcggc
     1201 ggatcaacct ggcgtcgccc gccgagagcc tgatggtcga gaagacgacg ggcgcggtgc
     1261 cgcacaccgg cggcaagcgg ttcgagctgg agagcgagta cggccggacg ctgctccgct
     1321 ggctgagcga gggcgcggcg aacgacccca agccgacgcc tgccgtgatt ggcgtcgatc
     1381 tctttccgcc gagcgccgtg ctggaaggcg aaggcgcgac gcagcaattc atcgcccggg
     1441 cccgctacgc cgacggcacc gaccgcgacg tgaccagcct ggcgacgttc ctgacgaaca
     1501 acgacaactc ggccccgatc tccgtcgatg gcctgctgac tgctggcgct cgcggcgaag
     1561 ccttcgtcat ggcccgcttc gagacgcaca ccgtcggcaa ccaggtcctc gtgttgccca
     1621 agggcctgat gtacgcggca ccgccggtca gcggcaacta catcgacgag ctggtcgggg
     1681 cgaagctcaa caagatccgc atcctgccga gcgaggtctg ctccgatgag gaattcctcc
     1741 gccgggcgac gatcgacatc gccggccaat tgccaacgcc ggaggaatac caggcgttca
     1801 tggccgatgc gtcgaaggac aagcgggcgc agctcgtcaa ccgcctgctc gagcggaagg
     1861 agttctcgga aatctgggcc atgaagtggg ccgagctgct catgatcaag tcgagcaacc
     1921 aggtcagcgc caaatcggcg ttcctgtatt actcgtggct cactgacaag attgccggca
     1981 atgtgccgct ggaccagatg gtcgccgagc tgctctcggc cacgggcggc acgttcaaaa
     2041 gcccggccac gaactattac cagatcgagc gggacacgct gaagacggcc gaaaacgtcg
     2101 ctcaggtctt cttcggcatc cgcacgcagt gcgcccaatg ccacaaccat ccgttcgacc
     2161 gctggacgat ggacgactat tacagctttg ccgcgttctt cgcccagatc ggccgcaagc
     2221 agagcgagga ctatcgcgag attatcgtct tcaacagcgg cggcggcgag gtgcggcacc
     2281 ccgtcggcgg ccgcaacatg ccacccaagt tcctcggcgg agccgcgccg gataccgccg
     2341 gcaaggaccg ccgcgagatt gtcgcccagt ggatcgcctc ggccgacaat ccctatttct
     2401 cgaccagcgt ggccaaccgc gtctgggccc actttttcgg caagggcatc gtcgagccgg
     2461 tcgacgacat ccgcgtcagc aacccgccca gcaaccccga gctgttccag gccctcggta
     2521 aaaagctcgt ggaatacaag tacgacttcc ggcagctcgt ccgcgacatc tgcagttcca
     2581 acgcttacca gcgaacggcg acgcggaacg aatcgaacgc caccgacgag cggaattttg
     2641 cccacgccaa cgtccgccgc atcccggccg agcagttgct cgattgcgtc agccaggtga
     2701 ccggcgcgag cgacaagttc cagggcctgc cgctgggtgc ccgggccgtc gagatcgcca
     2761 acggatcgac cagcaactac ttcctgacga cctttggccg cgctccgcgc gagacggtct
     2821 gcgcctgcga ggccaagacc gagccgaccc tctcgcaagc cctgcacatg ctcaatggca
     2881 acacgatcca gggcaagatt cagcagggcg gcgtggtgaa gaagctgctc gacgcggggc
     2941 agacgccgcc gcaggtcatc gagtcgctct atatccgcgc cctggcccgc aagcccacgc
     3001 ccgaagaagc cgagcggctc ttgaccgtcg tcggccaatc cgccaatccg cagcaaggcc
     3061 tggaagacgt cttctgggca gtgctaaact cccgcgagtt tattttcaac cactgattcg
     3121 tagagcaagc gccctcgctt gctcacccca attccatttg aggactgcac aagcggggac
     3181 gcttgtgcta cgagaccgcc gccaactgag aagagccatg cgcctgttcc ttgcttccct
     3241 attcgccgca gcgatcggca cgacgacggc tgccacggcg caggagtcca aacccgcggc
     3301 ccccgctggt cccaggatca cgtacgacga ccacgtccgg ccggtccttc gggagcactg
     3361 cttcacctgc cacagcgccg acaaacagga aagcggcctg gccctcgaca cctacgccaa
     3421 ggcgatggcc ggcggctcca gcggcgagat cgtcatcgcg ggcgacctgg gcagctcgcg
     3481 gctgtgggcc ctggtcagcc atgctgaaga tcccaagatg ccgcccaagc aggacaagct
     3541 gccggcggcg aagctcgagc tgatcagcaa gtggatcgag cagggcgcgc cggaaaacgc
     3601 cggctccaag gtcgccatca aaaagaaccc gctggccgtc gcggccgtgg gcaagagcgg
     3661 caagccgtcc ggccccgcgg cgatgcccga aggtctgttc aagcagccgg tcgtcgtcac
     3721 cgagaagccc gggcaaatca cggcgctggc gagcagcccc tgggcgccgc tcgtcgccgt
     3781 agcggggcag aagcaggtcg tgctctatca atcggacagc ggccagctct ctggcgtgct
     3841 gccgtttccc gagggcatcc cgcacgtgct gcggttcagc cgcaacggca gcgtgctctt
     3901 ggccgcgggc ggccgcggcg ggcattcggg ctgcgtggcg ctttatgacg tgaagagcgg
     3961 acggcggatc gccaagatcg gcgacgagct ggacgcggtc ctcgcggccg acatcaacag
     4021 cagccacacg ctggtcgccc tgggcggacc gaaccgcgtg gtgcggatct tttcggttca
     4081 gacgggcgag ctcttgcacg agattcgcaa gcacaccgac tgggtctatt gcgtagagtt
     4141 cagccccgac ggcgtgctgc tggcgaccgg tgaccgaagc ggcggcctgt ttgtctggga
     4201 ggccgacacg gcccgcgagt atctcaacct tcgcggccac aacggcgcga tctgcgacgt
     4261 aagctggcgg ctggattcga acgtcctggc cagcgccggc gaggacacga cgatcaagct
     4321 ctgggagctc aacgacggca acgcgatcaa gtcctggaat gctcacggcg gcggctcgtt
     4381 ctgcgtggag ttcactcacg acggccgcct ggtcagcgcc ggccgcgata acacggtcaa
     4441 gacctggaac ggcgacgggg cggcgcaaaa gtcgttcccc gctttcccgg aagcggccct
     4501 ccgctgcacg ttcacgcacg acggcaaccg cgtcgtgggc ggcgactggc tggggcacgt
     4561 gaacgtctgg gacgccggcc aggcgacgct ggtctattcg ctggccccca acccgccgac
     4621 gctcgccaaa cgggtcgagt tggccaaggc tgatttgaca gcgaaacagg ccgccgttgc
     4681 ggccgtcaat gccgaattgg ccgcagtcac gaaggttgct accgacaagg ccgccgcact
     4741 gaaagccgcg accgacaagc aggccgccgc ggctgcaacg gcacaaaagg ctgaagccga
     4801 tcggctggcc gcagtcaaag cagcggaaac agctgcggct gcggtgaaac ccgcgaccga
     4861 tgcagcggcg gccgcccagg ccgcgctcac cgcgccgacg gccgagaagg ccgccgccga
     4921 caagaccgtt gccgacaaaa cggcagcggc gaagaccgcg gccgacaagg ctgctgtcga
     4981 caaggcgggt gcggataaac tcgcagcaga aaagaatccg gaggcggcca aggcattgga
     5041 gacggctgcc gcgtcgcagg tcgtcgccgc tcagtcggaa gtcgagcggg cccaggccgc
     5101 cgtcgttgcc gctcagaagg ccgaggcgct taccaaggct gccaatagcg ccgcggcggc
     5161 cgctactgcc gccgagaagg ccagggcgga cgccgcccag gccgctaagc aggtagagac
     5221 aatgacttct gcggccaaag ctgcagccga tgccgtcccg tccgccaagg ccgaggttga
     5281 caaagcaacg aaagagaaat ccgatgccga cgcccaggtg gcggcgaaga aacccggcgc
     5341 cgacgccgct cagactgcgg tgaaggccgc cgaggcggcg ctgcagcaag ccgaaaccga
     5401 cctgaagaat ttcagcgaga cgtcggccaa gctcgcagcg gcggcgaagg ctgccgccga
     5461 ccaggtcgcg gcaaagcaag cggccgtcac cgcggctgag tccgagacga aggccacggc
     5521 ggatgccgcg gcccagaagg ctgccgccgc caaaaagctg gccgagcagc tcgccgcgct
     5581 acaggccgag ctggccaaag cccaatccga ccagaaagcg gccgacgatg ccgcggccgc
     5641 caaggccaag gccgtcgccg acgcacaggc cgctctcgcc gcagccaaag cagcggctga
     5701 tgcagtcgcg gccgagcaga aggcgtttgc cgacgcctac ggcccgaaat aggcgcgaaa
     5761 aaaagggcag gcgaaacgcc tgccctgctc tctgctctgt tcaatctgtt ctggctacat
     5821 cgaatcgcgc ggacggatcg cgccgaccag gccgctgtag tcgtagcggt cgctgtcgtg
     5881 ccacaggggc agtccgagct ccagccgcct gcggaggatt tcgatcttct cctgcgagcc
     5941 ggccggggcg tcggtggcgt cgaagttcga cttggaatcg gggagccaat cctcgtcgtg
     6001 cccgtacttc aaaatggcct caaacacgtt cttgcagcgt tccatggttt ctccaaatgg
     6061 ggtcaatacg ccaagcccgg cggtgttaac atggcagtca agccagaggg ccgcagcgga
     6121 acatccgcgg gcggcaagtt cacctggtgg cctggcgaaa acggtttcct cggcggtcgc
     6181 aaatcggctc tcaagcgggg gagagcggcc ggcagtttgg ccggcgattc ctgacgacca
     6241 gcgttgtgtt cggccaatta ttcttggagg gtgcctcgcc gtcaagcttt ttgaggcctc
     6301 aaatcggagt tttgtgacgg cgaaaaacac gccgcagtgc cgcccgcgcg gagtaggcaa
     6361 atgttgaggc gcttggccca aggaacgacg gcaaatgacg taccaacaat ggcttatgga
     6421 atctctggca gtccattgcc caccggttgg aaacattgtg aaatcaagaa cgaggtttta
     6481 acttccataa ggccataagc aagggagcct acttgacagt cggtggacag ccacgtcaag
     6541 tcaaactcga agacccgcgg acagacctct cggcccgacc ttcgcgccct ccttgcgaag
     6601 atcgcggctg gacggatgca gcgccccgca gcatcctatt tcggggtggc ccgggagcga
     6661 gggacggcgc ggaacatcct cgatggacac ttggtaaatg tgaaaagtcc atgtcttggc
     6721 ttcaaaagca cctcgccctg acgcctcgct cgcggggatg ccatttgatc acccgcgagg
     6781 tcgagcaggc gctgccggaa atcgcttcgc tgcgggctgg gatcctgcac gtcttcattc
     6841 agcacacgtc cgcctccctg acgatcaacg agaatgccga ccgcgacgtg cgggtggatt
     6901 tggaaagctc gcttaatgtc ctggccccgg aagactttcc ttacgtccat acgctggagg
     6961 gtcccgacga catgccggcg cacctgaaag cgtcgctgct gggctcctcg gtgacgattc
     7021 ccgtcggcgg cggcaagctg ctcctgggca cgtggcaggg catctatctc tgcgagcacc
     7081 gcaatagcgg tggcagccgg aacctggtgc tcaccttgtg gggcgattag acgggaacct
     7141 tgcctcgcgc agcggatccc agctcatcga gccaccactc gatgaagacg gcctggttga
     7201 cgttggcgtc gatgtagcca taggcgtcca ggcagaagtc gaggcaagcg gcggccgctt
     7261 cgtccccggg cagccagctc gcggcctctt tgaccgcagc ggccaactcc gcgtcggcgg
     7321 gcgttcgacc agcggttatg gagaggaggg cggcccggta gaattcctcc gccagcgaca
     7381 cggccagccg cagccgctcg cgcttgagcg ccgactcctt gccggcgtcg tcggcgaatt
     7441 ggccgatcag cttcgctgca gcaagggggt cgaagtcgcc gcggctgagc aggcccagca
     7501 gctggccgcg aaattcggcc atccgttcgt cgcaccagag ctgtgcgagc tgcagcgagc
     7561 cgccggagcg ggcggcggcg cggcgagccg tcgccggatc gtcgcagaga cgtcgctcca
     7621 ggagcagctc ggccacgtcg ctttccgcca gcggttgaaa gcggacgatc tggcaccgcg
     7681 agcggatcgt ggggagctgc cgctgctcgc tggttcccag caggatcagc agcgacttcg
     7741 gcggcggctc ctcgagcgtc ttaagcaggc agttggcgcc ttctttgttg agatagtccg
     7801 catcatcgag tatcgcgacc ttgtggcggc cggaataggg cttgcgcgag atgttataga
     7861 gcagcccttc ctgcatccgg tgttctttgt cgccgatcaa gagttcgacc gggatgaagc
     7921 tcttgtcggc cggcttgccg atcagttcca cgtcgggatg cgagccggcg agcacctggt
     7981 ggcagctgcg gcactggccg cagggatcga gccgctcctc agccgcgcgc tcgcacagga
     8041 ggccctgggc cagctgcaag gcgaaggccc gcttgccgat tcccgccggg ccgacgaaca
     8101 ggaacgtgct gaccagccgg ccgcgggcca gcgcctggcg gaagcgctcg gcaatggcgt
     8161 cgtgcccgcg gatcgattgc caggacatgg ccgggagtcg ggagtcgggg gtcgggagtc
     8221 ggctattgcg gcgtgggcgg cgtcgtcgtg atcgccgtga cttcccagtg ggcgtggtgc
     8281 gacttctcct ccacgcggcg ggcgaggctg atccacagcg gcaagtcgcc gccgcgctcg
     8341 gcgggccggg acagtacgta gcggagctga atctggtcgc tgaacccttc attataagtg
     8401 accgtcacgc cttcgtagcg gaccgatccc tgcgggccgg cggcgaccag gtcggcgatg
     8461 cccttctggg ccttgaacga ctgcagctcc tgccgggcct cggcgttctt ctcgtagtag
     8521 tccttgaccg cctccgcgtc ggcaatccgc gcctgcggcg ccatccgcag ctggtgggcc
     8581 tcctcgagct tgccagcctg caccagcact agccagcctt ccgtggcccg ctgggcggcc
     8641 gattccagcg cggcctgccg gctgaagtgc c
//
LOCUS       DASRJB010000326         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_92643, whole genome shotgun sequence.
ACCESSION   DASRJB010000326 DASRJB010000000
VERSION     DASRJB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92643"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VFB80_08760"
     CDS             <1..1470
                     /locus_tag="VFB80_08760"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HZN33898.1"
                     /translation="LLPLVARPDESPRPTAAPLALKNGWLTIGGKLVTGGRQRTVWWR
                     GSVLPSRAGELGVGITRFVPGREGPGFTDDLDALTNEMVASRRAAIEHHWGLWYDRRR
                     DDHQMVRRIDGEAWPPFYEQPWARSGQGTAWDGLSKYDLTKFNPWYFSRLKTLADLCD
                     RKGLVLIQQMYFQHNILEAGAHWADFPWRPANCLQPTGFPEPPPYVGGKRIFMADAFY
                     DVTHPPRSPPAETLRRELHRAYIRHCLDVLGGNSNVIFMTGEEFTGPLAFVQFWLETI
                     REWQQETGREVLVGLSCTKDVQDAVLADSQLSGLVDVIDLKYWWYAADGSLYAPAGGE
                     SLAPRQQLREWRGSKSRSDGQIARQIRECRSRYPDKAVLCSLEPAGGWTVAAAGGSLA
                     HMPRLDNDQLLAALPRMLPVERSGQWLLADPGNDYFLVATGGKTELDLTNERGEFDCR
                     WIDLRTGRMRPHPASVSAGTTIELTPPEASAWGIWLSRK"
     gene            1493..>2100
                     /locus_tag="VFB80_08765"
     CDS             1493..>2100
                     /locus_tag="VFB80_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33899.1"
                     /translation="MHNASPRPPRAAFTLVELLVVIAIIGVLVALLLPAVQAAREAAR
                     RSSCSNNLRQIGLAVHGFNDTYNKLPYLRSGGGQNRHTWALLILPYMEQGNIFQVYST
                     PITGVSQTDGMNNHTATDPAIVNVRGAQIKIYHCPSRRATKSLSPIQMGSTVLGLPSD
                     YAACSGDTGTAPTTGVFPAVNSNHLLAGIRLAEIRDGTSNTI"
BASE COUNT          374 a          698 c          696 g          332 t
ORIGIN      
        1 cttttgccac tcgttgcgag gcccgacgaa agccctcgcc cgactgctgc gccgctcgcg
       61 ctgaaaaacg gctggctgac catcggcggc aagctggtga ccggcggccg gcaaagaacc
      121 gtctggtggc gcggcagcgt gctgccctcg cgggccggtg agttgggcgt cggcatcacg
      181 cggttcgtcc caggccgcga aggcccggga ttcaccgacg acctcgatgc cttgacgaat
      241 gaaatggtgg ccagccggcg ggctgcgatc gagcaccact ggggcctgtg gtacgaccgc
      301 cgccgcgacg atcaccagat ggtccgccgc atcgatggcg aggcctggcc gccgttctac
      361 gagcagccgt gggctcgcag cgggcaggga accgcctggg acggcctgag caaatacgac
      421 ctgacgaaat tcaatccctg gtatttctcc cgcctgaaga cgctggccga cctctgcgac
      481 cggaaggggc tcgtgctgat ccagcagatg tactttcagc acaacatcct ggaagccggg
      541 gcgcactggg ccgactttcc ctggcgaccg gccaactgcc tgcagccgac gggcttcccg
      601 gagccgccgc cgtacgtcgg cggcaagcga atcttcatgg ccgatgcgtt ctacgacgtg
      661 acgcatccac cgcgcagccc gccggcagaa acactgcggc gcgagttgca ccgggcctac
      721 atccgccact gcctggacgt gctgggcggc aactcgaacg tgatcttcat gaccggcgag
      781 gagttcaccg gcccgctggc gttcgtgcaa ttctggctgg agacgattcg cgagtggcag
      841 caggaaacgg gccgcgaggt gctcgtgggc ctgagctgca ccaaagacgt ccaggatgcc
      901 gtgctggccg attcgcagct gagcgggctg gtcgatgtga ttgatctcaa gtactggtgg
      961 tacgcggcgg acggcagcct gtacgcgccc gccggcggcg agagcctggc tccgcggcag
     1021 cagctgcgcg agtggcgtgg cagcaagagc cgctccgacg ggcaaattgc ccggcagatc
     1081 cgcgaatgcc gcagccgtta cccggacaag gccgtcctct gctcgctgga gccggcaggc
     1141 ggctggaccg tcgccgccgc aggcggttcg ctagcgcaca tgccgcggct ggacaatgac
     1201 cagctgctgg cggcactgcc gcgcatgctg ccagtggagc ggagcggcca gtggctgctc
     1261 gccgatccgg gcaacgacta cttcctggtc gctacgggtg gcaaaacaga gctcgatttg
     1321 accaatgagc gcggcgagtt tgactgccgg tggatcgatt tgcggaccgg ccggatgcgg
     1381 ccgcatcccg catccgtttc ggccggcacg acgatagaac tgacgccgcc ggaggcgtcg
     1441 gcttggggga tttggctcag tcgcaagtag aattagagat tggagaatcg tgatgcacaa
     1501 tgcctctccc cgcccgccgc gagcggcttt cacgctcgtg gagctgctcg tcgtcatcgc
     1561 catcatcggc gtgctggtcg ccctcctgct accggcggtg caagccgccc gcgaggccgc
     1621 ccgccgcagc agttgctcga acaacctgcg gcagatcggc ctggccgtac acggcttcaa
     1681 cgacacctac aacaagctgc cgtacctgcg ctccggcggc ggacagaacc gccacacttg
     1741 ggcgctgctc atcctgccgt acatggagca ggggaacatt ttccaggtgt atagcacgcc
     1801 gatcaccggc gtcagccaga ccgacggcat gaacaaccac acggcgaccg atccggcgat
     1861 cgtgaacgtg cgcggagcgc agatcaagat ttaccattgc ccgtcgcggc gggcgacgaa
     1921 atcgctcagc ccaatccaga tgggcagcac cgtgctggga ctgccctcgg attacgcagc
     1981 ctgcagcggc gacacgggca cagcaccgac caccggcgtg tttcccgcgg tcaatagcaa
     2041 tcacctgctc gccggcatcc ggctggccga gattcgcgac ggcacgagca atacgatcat
//
LOCUS       DASRJB010000327        13102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_93138, whole genome shotgun sequence.
ACCESSION   DASRJB010000327 DASRJB010000000
VERSION     DASRJB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93138"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFB80_08770"
     CDS             <1..301
                     /locus_tag="VFB80_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN33900.1"
                     /translation="KQAQRRGGDRQRQDLDLVQLSAPAPPAELLALDEALAALAGQDA
                     VKAELVKLRYFAGLSMEEAALALGISPATAHRYWNYARAWLHQQIAGQPADGQES"
     gene            392..5827
                     /locus_tag="VFB80_08775"
     CDS             392..5827
                     /locus_tag="VFB80_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33901.1"
                     /translation="MSAALPRTEELIFAEALDLPLAAREAFLAAACNGDAALLARVRR
                     LLQSHTGAGDFLKEPPAATIEQPSPERPGTIIGPYKLLQQIGEGGMGVVYMADQEQPV
                     RRRVALKIIKPGMDSGQVIARFEAERQALAMMDHVNIAKVLDAGTTSEGGRPYFVMEL
                     VHGVPLTKFCDENRLTLRERLQLFVPVCHAIQHAHQKGIIHRDIKPSNILVTMYDDRP
                     VPKVIDFGVAKAIEQRLTERTLFTQYGMLVGTFEYMSPEQAEMNAFGVDTRSDIYSLG
                     VLLYELLTGTTPLEGRRLRQAAFVEVVRLIKEEEPPRPSIRLSSTGTLPKVAAACRTE
                     PARLAQLLRGELDWIVMKCLEKDRTRRYETANGLARDVERYLHDEPVEACPPSAAYRL
                     RKFARRYRAALCTAALVAASLLAGIIASSVLAWQARKSQLQAERNRQAAVRGEQQAQI
                     ARQAEAAARSGAEAERDKAGKLNQALESVLKDQRRTIYAAELNLVRLEAQRGSLPRMR
                     ELLMRQIPGLDQEDLRGFEWNYWYRFLHRAQELRRFDDLAISAGTDRVVMVPGGGLVA
                     VARGGQTQLVEVATGTVKYSAPTKLTWGVNRNPLTSAGQLVAGGAVSGAAYWPSANSR
                     LATEFEVWDPQGSRRTIKLPEGSLSHLSFLTISNDGRFVAALGNDGSHTRTSPVCRLL
                     TWNAATGESQLDLTIPGEFNRLRFSDDASLLAAYLCHGSQRSQNDLREVITVLDRVTG
                     KRLATCHHDDDADSVFFLPGGKGLLFGTLGWSGRNRKELYRWNIGDEPPRRLGLEYMP
                     DYMQGAVSPDGSLLAISGHTVSTVRLIDTSLGLVVSTLHNEASTIDSLTFSPDGKRLL
                     ACDTAGVVVAWDLDRDQDLFKLRTNPLGPLAGSGYALSADQSLLAVATIYDGVRLRRR
                     DGHETILRPDDPTTSLRNATVRLRFSPDRRWLAYESRSDFASVTPTGVIASGKTLRLF
                     DTLTGMESWTVRLPEFRPSAAPPAPVNWGVPDFLPAERWAFTADGSRLAVLRGEQLYL
                     IDAATGSVQTSPAVLTKDPLVHRRQLVRHSASGQLLLAVVEPAGGGFGVLRMLDAISG
                     ATVGETRVPLTQRIDDVVAAPDGRHVGLIRGETLRLEVWDLSARRQTLEAPGRYLVFS
                     NDGSKIAALSVIAKTVAGVARPLERVDQASLWEVATGRQLSTLSLTGNGAEEVVFSPD
                     GRRLLTLHGKLSSGSGGAVPEGRLWDVLSGREMMTLPVADVNHYLWELAFDPSGTRLT
                     SLVLVKVAGNGGGMGAIVYEATPLSEAEDALLLARPLVADLFQQHPLVSDVTAALEAN
                     LRLRPAVRTAALSLARQRSDDPQAIAAACQALVVIPNSTAEQAARALRWAQALQRLLP
                     HDPRSTLLLGAAQFRAGQLPAAQATLQAELRAGAETEASKLAKLKVTQRAFRVLILVR
                     QQSQEPALSEVVLMLRDLRRIVRFSSDQELSAAIAPSSEYAVVGEALGAVNSLAGAAP
                     LVGQPPLVGARNLIAVIDANDDGRFTSADDPQDWEELQIFDTNRDNEVTAEEFAVTFG
                     GAMSPGADPSRQLEMLARFIERSPKNADLYFQRAIVLNREGKYDQSLQDLNVGLDLKP
                     QDAEAHNLRAVARIFLGDLEAAMDDCNRSIALNSKSANAFHNRGTIHKLQGRLDEALL
                     DLNEALRLTRAARTLVARGDVRRLQGRYQEALRDFEDSLAADDKTATAFAARSRLRAT
                     CPVASFRNAKQALADGQKACETSKWKDGDALDALAAACAESGDFAQAASYQQKAIPLA
                     MQPLRADYEARLALYRAGQPFREGTSQ"
     gene            6129..6464
                     /locus_tag="VFB80_08780"
     CDS             6129..6464
                     /locus_tag="VFB80_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HZN33902.1"
                     /translation="MSISNVAKNEGKKGKPNLYTFTVTLSAAYDQAVTMSFSTVNGTA
                     TTSDNDYVARTGTLIFNPGETTKTITIEVKGDSKKEANETFYLDLFDNSDNSLFTKSR
                     GLGTILNDD"
     gene            6813..12023
                     /locus_tag="VFB80_08785"
     CDS             6813..12023
                     /locus_tag="VFB80_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33903.1"
                     /translation="MSNLHKQLPRRGFVGISPMGWPPGTRRLRRHFGNRAPVWRYRPL
                     GIESLEDRALLTVSLSGVPLWIEQGPGTMTNGSSEGIPDEPVAGAVNVVAPDPVNANR
                     LFAGSVNGGIWRTTDATSPNPHWTPLTDQFRSLSVTSLEFSPLDPAHNTLFAGTGSTS
                     SGGGDGGPAIGLLKTTDGGQTWSVLGGPVTGSGANFGILQVIPTAIGTPATQVVLVST
                     SAGVFRSTDGGVTFGMSPVLSGSVTHLVADPANAGRLFAAVPGQGVFRSPDQGQTWDR
                     VLGPTLSVGGFGADYDFLEIAVHYNAGSGTNAIYVAGFFDPEDDSNDDDDVIVLRAPD
                     PGAEVVEWTLMGLPARLGIPRQPSIAAHPANENLVFISSVHGDNLAGCERSGARTSRG
                     DASLDLASQWIQVVCDGASGTAPHVDSRGLVFDAYGNLLETTDGGIYRLRNPASDYSP
                     GTEPFWESVNGDLRTVEFYYVAYDSLNHVTFGGNQDNGTPHQLSPGTPGGIPDDFTWE
                     RRVIGDGGVVQVDQSNQNCLVADPCTIRYQSFHFLGGFTREEFDGNNAPVGMHDIQLE
                     IEDSEGRRLTDGQGTLSDASDDYDVTTQFIQAWALNAADPMRLLIGTSYLYESIDQGD
                     HLTAIGGLDDDFSPINPVGAVNRTMGSGSGLGQASPIAYGGWIGNVPYPDVLWIGATQ
                     GLDKEDNDGDGDTDESDELTPTLRLRTSGSGLPDIVANYPGGQIRDIALDPIDWRQAY
                     ILDASGSVFRAVTDSDGAKISFTDLTGDLTGDLAMFATDNPTIDLRTIEFMRADANTP
                     VLLVGGQGGVYRSINFGAWSELGVNLANAPVTDLHYDALDNVLVAGTFGRGAWTIPLA
                     SLNLFASPSVDFCGTDNDDLMVLDTNAGNPLLLDLVLNGVTEASLPYSVLKTVHVCGR
                     DGDDVILTRAMQPWIFLSVDGDADHDIIGFGNEVTIPVVASGGSGNDIIDGTSESDIL
                     NGDSGDDHLAGLGGDDLLNGGPDDDTIFGGDDDDLIDGGQGDDSLDGGRGNDNIAGDI
                     RFVLGEIGGNDTIVGDSGNDIIDGDGGDDSLDAGSGEDTLDGGDGNDVLVGGSDADSL
                     SGGDGNDQLLGGMRIASLDALEADPSADTLSGGAGNDTIIADDGSFLPLIVRETIGGN
                     DVVRGGSGNDLIFSGFGNDNVQGQSGDDTIAAGPGNDTVIGGWLIFVPFPPLGDGDDF
                     INGGVGADLLYGDNFDPLLPPYVSLSGGDDELLGGDGDDTIYGQAGHDNLDGGADDDA
                     LHGGVGNDELTGGPGTDGLVGDVGTDLLFGNQGPDTLDGGADNDVLVGGSDDDELQGG
                     SGDDVLTGEAGDDLLVGGPGFDTLDGGADDDGLIGGNLGGAGVQADFSPDSLNGGDGD
                     DVLLGDSGSITPFFIPAFDIFGGGDSIQGGPGNDISYGQAGNDFIGGGPGNDIIVCGV
                     GNDIASGDEGSDIVVGDAGDDLLAGGDANDTLTGGSGNDLLVGGVFSAGAMVIAETGD
                     DLLQGDSGEDILFGDSWALGDPLNLGVSGGHDTLRGGPGSDLAVGQTGNDLLFGDAGD
                     DTLLGGDGNDRIRGGDGDDQLGGGAGHDLLLGEVGQDALAGAGGRDVLIGGQGADTLD
                     GGDDDDILIAGTTAHDANDAALNLILAEWTSLRDYESRVKNLKGTNNPEFDNRLNAEV
                     FLKKNGNSNNGNGNNGNGKGNNGNNGNMPTVFDDDASDELTGSSGDDWFLYEPALDEL
                     VDWAGGENKN"
     gene            complement(12061..>13102)
                     /locus_tag="VFB80_08790"
     CDS             complement(12061..>13102)
                     /locus_tag="VFB80_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN33904.1"
                     /translation="LGAAMQHAGTIQHVSFRRDSAVLVTASNDGTAQLWDAASGEPSS
                     PALDHRSRAVVAAVFSPDGRVLATGDSAGIVRIWNGETGELVHAFESASGPANSIAFS
                     PDAARVVVGSASPDNGVRVWDAKSFELLWKSTEATGHRGRVRMVSVSPNGRVIASASN
                     DETVKFWDAESGQRIGEPLPHGGEVFVVAFSPDSQLAVTGGYDATVRLWKVPTGQLVG
                     EPMRHAGLVTAAVFSPDGHTLLTGSADWSARLWDVTTCLPLCPPVRHNGELMSVALDR
                     TGRIALTGRLWHLPAPLPDDQALIDLWVQLATQRKLAAGGNIDWLDPAALAKLASEFH
                     TRTGRSWSEWAD"
BASE COUNT         2353 a         4286 c         4352 g         2111 t
ORIGIN      
        1 gaagcaggcc cagcggcgcg ggggcgaccg gcagcggcag gatttggacc ttgtacaact
       61 gtcggcgccc gccccgcccg ccgagctgct ggccttggac gaagctctgg cggcgctggc
      121 cggccaggac gccgtgaagg ccgagctggt caaattgcgg tattttgccg gtctgagcat
      181 ggaggaggcc gccttggcgc taggcatctc gccggccacg gcacaccgtt attggaacta
      241 tgcccgggcc tggctgcacc agcaaatcgc cggccagccg gcggatgggc aggaatcttg
      301 aaggtttgct gagagagact gaccgcaaac tacgcactgt agtcaggaca gcactgtaat
      361 caggagaccg tcgcccaggg ggaagccaaa catgtccgct gcgttgccca ggaccgagga
      421 gttgatcttt gccgaggctc tggacttgcc actcgcggcc cgcgaggctt ttttggccgc
      481 ggcctgcaac ggcgatgccg cactcttggc ccgcgtccgc agacttctcc aatcgcacac
      541 cggagcgggt gactttctga aagagccgcc ggcggcgacc atcgagcagc cctcgcccga
      601 gcgtcccggc acgatcatcg gcccttacaa gctgctccag caaatcggcg aaggcgggat
      661 gggcgtggtc tatatggcgg accaggagca gccggtccgc cgccgggtgg ccctcaagat
      721 catcaagccg gggatggaca gcgggcaggt aatcgcgcgg ttcgaggccg agcggcaagc
      781 gctggcaatg atggaccatg tgaacatcgc caaggtcttg gacgctggta cgacgagtga
      841 aggcggccgg ccctacttcg tcatggagct ggttcacggc gtgccgctga cgaagttctg
      901 cgatgaaaac cgcctcacgc tccgcgagcg gctgcagctc ttcgtaccgg tctgccacgc
      961 catccagcac gcccaccaga aaggcatcat tcaccgcgac atcaagccca gcaacatcct
     1021 ggtcacgatg tacgacgatc gccccgtgcc caaggtcatc gacttcggcg tggccaaggc
     1081 catcgagcag cggctgacag aacgaacgct gttcacgcaa tacggcatgc tggtcggcac
     1141 cttcgagtac atgtcgcctg agcaggccga gatgaacgcc ttcggcgtcg atacgcggag
     1201 cgacatttat tcgctcggcg tactgctgta cgagctgctc accggcacca ctccgctgga
     1261 aggccggcgg ctccgccagg ccgcgttcgt cgaggtcgtg cggctgatca aggaggagga
     1321 gccgccgcgg cccagcatcc gcctgagcag cacggggacg ctgcccaagg ttgccgcggc
     1381 ctgccgcact gagcccgcgc gcctggccca gctcctccgc ggcgagctgg attggatcgt
     1441 gatgaagtgc ctggaaaagg accgcacgcg gcgctatgag acggccaacg gcctggcccg
     1501 cgacgtggag cgctatctgc atgacgagcc ggtcgaagcc tgtccgccct cggccgcgta
     1561 ccggctgaga aaattcgccc gccgctaccg ggccgcactc tgtacggcgg cgctggtggc
     1621 ggcatcgctg ctggccggga tcatcgccag ctcggtgctg gcctggcaag ctcgaaaaag
     1681 ccaactgcag gccgagcgga accggcaggc ggcggttcgc ggcgagcagc aggcccagat
     1741 cgccaggcag gccgaagccg ccgcgcggag cggggccgag gccgagcgcg acaaagccgg
     1801 caagctgaat caggcgctgg aatcggtgct caaggatcag cgacgaacga tctatgccgc
     1861 ggagctcaac ctcgtgcggc tggaggccca gcggggcagc ctgccacgga tgcgagagct
     1921 gctgatgcgg cagattccag gactcgacca ggaagacctc cgcggtttcg agtggaatta
     1981 ctggtatcgc ttcctgcacc gtgctcagga gctgaggcgg tttgacgacc tggcgatctc
     2041 tgcggggacc gaccgcgtgg tcatggttcc cggcgggggc ctggttgccg tggcgcgcgg
     2101 cggacagacg cagctcgtcg aagtggccac ggggaccgtg aagtactccg cgccgaccaa
     2161 actgacctgg ggcgtcaacc gcaatccgct gacgtcggcc ggacaactcg ttgccggtgg
     2221 ggccgtttcc ggcgcggcct attggccgtc tgctaactcg cggctggcca ctgaattcga
     2281 ggtctgggat ccgcaaggct cgcggcgcac gatcaagctg cctgagggca gcttgagtca
     2341 cctgagcttc ctgacgatca gcaatgacgg caggttcgtc gccgccctgg gcaacgacgg
     2401 ctcgcacacc cgcaccagtc cagtttgccg gctgctcact tggaacgcgg ctacgggcga
     2461 gtcgcagctc gacctgacaa taccgggcga attcaatcgg ctgcggttca gcgatgacgc
     2521 gtcactgctc gctgcctacc tgtgtcacgg gtcgcagcgc tcgcagaacg acctgcgcga
     2581 agtgatcacc gtgctcgatc gggtcactgg gaaacgattg gcaacctgcc accatgacga
     2641 cgacgccgac agcgtctttt tcctcccggg cggcaagggc ctcttgttcg gcacgctggg
     2701 ctggagcggc cggaaccgca aggagttgta tcgctggaac ataggcgacg agccgccgcg
     2761 gcggctgggc ttggaataca tgcccgacta catgcagggc gccgttagtc ccgacggcag
     2821 cctgctggcg atcagcggac atacggtttc gaccgtgcgg ctgatcgaca ccagcctggg
     2881 cctggtcgtg agcaccctgc acaacgaggc cagcaccatc gactcgctga cgttctcgcc
     2941 cgacggtaag cggctgctgg cctgtgacac ggcaggcgtg gtggtggcct gggacctgga
     3001 ccgcgatcag gacctgttca agctgcggac caatccgctc ggaccgctgg ccggatcggg
     3061 ctatgcgttg tccgccgacc agtcgctact ggcagtcgcc acgatatatg acggcgtgcg
     3121 gctccgccgc cgcgacggcc acgagaccat cctgcggccg gacgatccga ccacctcgct
     3181 ccgcaacgcg acggtcaggc tgcggttcag cccggaccgc cgctggctgg cgtacgaatc
     3241 gcgcagcgac ttcgcctcgg ttacaccgac gggagtcatc gcgagcggca agacgctgcg
     3301 gctgttcgac acgctgactg gcatggaatc gtggacggtg cggcttcccg agtttcgccc
     3361 gtcggccgcg ccccccgccc ccgtgaactg gggcgtcccc gacttcctgc ccgccgagcg
     3421 ctgggcattt acagccgacg gcagccgcct cgccgtgctc cgcggcgagc agctctatct
     3481 cattgacgcc gcgacgggca gcgttcagac ctcgccggca gtgctgacga aagatccgct
     3541 tgtgcaccgg cgtcagctcg tgcggcactc ggccagcggg caactgctgc tggccgtggt
     3601 ggagccggct ggcggcggct ttggcgtttt gcggatgctc gacgccatca gcggcgcgac
     3661 cgtgggcgag acccgcgtgc cgctgacgca gcgcatcgac gacgtcgtcg cggcccccga
     3721 cggccgccac gtggggctga tccgcggcga aacgctgcgc ctggaagtgt gggacctttc
     3781 cgcgcggcgt cagacgctgg aggcgcccgg ccggtatctg gtgttcagca acgacggcag
     3841 caagatcgcg gcgctgagcg tgatcgccaa gaccgtagcc ggcgtggcgc ggccgctgga
     3901 gcgcgtcgat caggccagcc tgtgggaggt cgccacaggc cggcagctct ccacgctcag
     3961 cttgacgggc aacggcgccg aagaggtcgt cttcagcccc gacggccggc gcctgctgac
     4021 gctccacggc aagctgtctt ctggttccgg cggcgcggtg cccgagggcc gactgtggga
     4081 cgtcctctcg ggccgcgaga tgatgacgct gccagtcgcc gacgtgaatc actacttgtg
     4141 ggagctggcc ttcgatccga gcggcacgcg gctgacgtcg ctggtgttgg tcaaggtggc
     4201 cggcaacggc ggcggcatgg gcgccatcgt ttatgaagcc acgccgctca gcgaagcgga
     4261 ggacgcgctg ctgctggccc ggccgctggt ggccgacctg ttccagcagc atccgctagt
     4321 cagcgacgtg accgccgcac tggaggccaa tctgcggctc cgccccgcgg tgcgcacggc
     4381 ggccttgtcc ctcgcgcggc agcgcagcga cgatccacag gcgatcgcgg ctgcgtgcca
     4441 ggcgctggtc gtcatcccca attccaccgc cgagcaggcc gcgcgggccc tgcgctgggc
     4501 ccaggcactc cagcggctct tgccgcacga tccgcggtcc accctgcttc taggggcggc
     4561 acagttccgc gccgggcagc tgccggcggc ccaggccacg cttcaggcgg agttgcgggc
     4621 aggcgccgaa acggaggcct cgaagctggc gaagctgaag gtcacgcagc gggccttccg
     4681 cgtcttgata ctcgtccggc agcagagcca ggagcccgcc ctgtcggaag tcgtgctcat
     4741 gttgcgggat ttgcgacgaa tcgtccggtt ctccagcgat caagagctgt ccgccgcgat
     4801 cgcccccagc agcgaatacg ccgtagtcgg cgaggcactg ggtgcagtga attccttggc
     4861 tggcgccgcc ccgctcgtcg ggcagccgcc actggtcggc gcgcggaacc taatcgccgt
     4921 catcgacgcc aacgacgacg gccgtttcac ttccgcggac gatccgcagg actgggaaga
     4981 actgcagatc ttcgacacca atcgggacaa cgaagtgacg gccgaagagt ttgcggtgac
     5041 ttttggcgga gcgatgtcgc ccggcgctga cccctcccgg cagctcgaga tgctcgcacg
     5101 gttcatcgag cgcagcccaa agaatgccga cctgtatttt caacgcgcca tcgtcctgaa
     5161 ccgggaaggg aaatatgacc agtcgctcca ggatctgaat gtggggctcg acctcaaacc
     5221 gcaagacgcc gaggcccata acctgcgggc tgtggcgcgg atcttcctgg gcgacttgga
     5281 agcggccatg gatgattgca accgctcgat cgcgctcaat tccaaatccg ccaacgcctt
     5341 ccacaaccgt ggaacgatcc acaagctgca ggggcggctg gatgaggccc tcctcgatct
     5401 gaacgaggcc ttgcggctga cgcgcgccgc ccgcacgctc gtggcccgtg gcgacgtgcg
     5461 ccgcctccag ggccgttatc aagaggccct tcgcgatttc gaggactctc tcgccgcgga
     5521 tgacaagacc gccaccgcgt ttgccgccag gtcccggctg cgggcaacat gtcccgtggc
     5581 gagtttccgc aacgccaaac aagctctcgc tgacggacaa aaggcgtgcg agacctccaa
     5641 gtggaaggac ggcgacgcgc tcgacgccct ggccgccgcc tgcgccgaaa gcggcgactt
     5701 cgcgcaggct gccagctacc agcagaaggc catccctctc gccatgcagc ccctccgcgc
     5761 cgactacgaa gcccgcctgg cgctctatcg tgctggccag ccctttcgag aggggacctc
     5821 gcagtgatgg ccgcgagtcc gatgcagatt ccgcccaaac atcctccatt gatccagacc
     5881 tagcaatggt tatggccgcg tggccagcac tagatgactc gattcgcgcg gagattttgg
     5941 ctctggtctt gaagccgacg tagggctacc cctaccgagt cgagaccagc aagacgatca
     6001 ccgtcctggt caacggcgac cgagtcggcg agccgaacga gaccttcgtc gtcaatctca
     6061 gcgatccgac caacgcgacc atcgccgata gccaaggcct aggcagcatc gttgacgacg
     6121 agccgcgcat gagcatcagc aacgtggcca agaacgaggg caagaagggc aaaccgaacc
     6181 tgtacacctt caccgtcacg ctctcggccg cctacgacca ggcagtgacg atgtcgtttt
     6241 ccacggtgaa cggcaccgcc acgacgagcg acaacgacta tgtcgccagg accggcacgc
     6301 tgatcttcaa tcccggcgag acgacgaaga ccatcaccat cgaagtcaag ggcgacagca
     6361 agaaggaggc caacgagacg ttctacctgg acctgttcga caacagcgac aattctctgt
     6421 tcaccaagag ccgcggcctc ggcacgatcc tgaacgacga ctgatcagat aacggaaaaa
     6481 gcggccggca ccgcccggcg ctcggtctgc gcgccgaatg ggttgccgtc gtcaatatag
     6541 cgcggaccac gtcacgctcg gagatttacc ccgtttcgcc gccgttggga ccgaccacgg
     6601 agcccaatct ggcggcttca aggacccgac cgcagtcggt gtttgggcgg ggtggggccc
     6661 agcgatctgc gttgccacgc gatttttctt tccgcgtaag aagggccgtg atgcgcttgg
     6721 ggcggaaatt ggcattcact gagtatcgaa caggcaaagc ccacgttagt ggttggcgta
     6781 gccgatcgcc gttgccgctg gaaggccgat ccatgtcgaa cctgcacaag cagttaccac
     6841 gtcgcggttt cgttggcatc tcaccaatgg gttggccgcc gggcactcgc cgcctgcgac
     6901 gacacttcgg caatcgagca ccggtctggc gctaccgacc gctgggtatc gagtcgctcg
     6961 aagatcgtgc tcttttgacg gtaagcctta gcggagtacc gctctggatc gaacaggggc
     7021 cgggcacaat gacgaatggc agttcggagg gcatccccga tgagccagtg gctggcgcgg
     7081 tgaacgtggt cgcgccggat ccggtcaatg ccaatcgcct gttcgccggc agcgtgaacg
     7141 gcggcatctg gaggaccacc gacgccactt ccccaaaccc gcactggacg ccgctgaccg
     7201 accagttccg gtcgctgtcc gtcacctcgc tggaattcag cccgctcgat cctgctcaca
     7261 acaccctgtt cgccggcacc ggttccacca gcagcggcgg cggagacggt gggccggcca
     7321 tcggtctgct caagacgact gacggcggcc aaacctggtc ggtgctgggc gggccggtca
     7381 ctggctccgg ggcgaatttc ggaattctcc aagttattcc tactgcgatc ggcactcccg
     7441 ccacacaggt ggtcctcgtc tcgacgagtg ccggcgtctt tcgcagcacg gacggcggcg
     7501 tgacgttcgg catgtcaccg gtcctgtccg gcagcgtgac ccatctggtt gcggacccgg
     7561 cgaatgccgg gcggctcttt gccgcggtgc ccggccaagg cgtcttccgc agcccggacc
     7621 aaggtcagac ctgggatcga gtgcttggcc ccaccctcag cgtcgggggt tttggcgcgg
     7681 actacgactt cctcgagatc gccgtccact acaacgccgg gtcgggcacg aatgcgatct
     7741 acgtggcggg cttcttcgat ccggaagacg attccaatga cgacgacgac gtgatcgtgc
     7801 tgcgagcgcc cgaccccggc gcggaggttg tcgagtggac gctgatgggc cttccggcgc
     7861 ggctcggaat tccgcgtcag cccagcatcg ccgcgcatcc cgccaacgaa aacctggtgt
     7921 tcatttccag cgttcacggc gacaatctcg ccggctgcga gcgcagcggc gcccggacct
     7981 cgcgcggcga cgcctccctg gatctggcaa gccagtggat ccaagtcgtg tgcgacgggg
     8041 ccagcggcac agcgccgcac gtcgattcgc gcggactggt gttcgacgcc tacggcaacc
     8101 tcctggagac aaccgacggc ggcatctacc ggctgcgcaa cccggccagc gactacagcc
     8161 ccggcaccga accattctgg gaatccgtga acggcgacct gcggaccgtc gagttctatt
     8221 atgtggcata cgactcgctg aaccacgtga ccttcggcgg caatcaggac aacggcacgc
     8281 cccatcagct ctcgccgggc accccaggcg ggatccccga cgacttcacg tgggaaaggc
     8341 gcgttattgg ggacgggggc gtcgtgcaag tggaccagag caatcagaac tgcctcgtgg
     8401 cagacccctg cacgatccgt taccagagct tccattttct cggcggcttc acgcgcgagg
     8461 agttcgatgg caacaacgct ccggtgggca tgcacgacat ccaattggaa atcgaagact
     8521 ccgagggacg ccggctcacg gacgggcaag gcacgctatc cgacgcaagc gacgactacg
     8581 acgtcacgac ccagtttatt caggcctggg ctctcaatgc cgccgatccg atgcggctgc
     8641 tgatcggcac cagttacctc tacgaatcga tcgatcaagg cgaccacctc accgcgatcg
     8701 gcggtctgga tgacgatttc tcacccatca atcccgtggg cgcggtgaac cgcaccatgg
     8761 gctcgggcag tggcctcggt caggccagcc cgatcgccta tggcggctgg atcggcaatg
     8821 tcccctatcc cgacgtgctc tggatcggcg ccacgcaggg cctggacaag gaagacaacg
     8881 acggcgacgg cgacaccgac gaaagcgacg agctgacgcc cacgctgcgg ctgcgcacga
     8941 gcggcagcgg tctgcctgac atcgtggcca actaccccgg cggacaaatt cgcgacatcg
     9001 cgctcgatcc gatcgactgg cggcaggcgt acattctcga tgcttcaggc agcgtctttc
     9061 gagccgtgac cgacagcgat ggcgccaaga taagtttcac tgacctgacc ggcgacctga
     9121 ccggcgacct ggcgatgttc gccaccgata accccaccat cgatctccgc acgatcgagt
     9181 tcatgcgtgc ggacgccaac acgcctgtgc tgctcgtggg cggccagggc ggcgtgtacc
     9241 gatcgatcaa tttcggcgcc tggtcggagc ttggcgtgaa cctcgccaat gcaccggtca
     9301 ccgacctgca ctacgacgcc ttggacaacg tgctagtggc aggcacattt gggcgcggcg
     9361 cctggaccat ccctctggcg tcgctgaatc tcttcgcaag cccctcggtc gatttctgcg
     9421 gcaccgataa cgacgacctg atggtgctcg atacgaatgc aggaaatccg ctgctcctgg
     9481 acctcgtgct taacggcgtc acggaagcct cgctgcccta ttcggtcctg aagaccgtcc
     9541 acgtctgcgg gcgagacggc gacgatgtga tcctgacgcg cgctatgcag ccgtggatct
     9601 tcctgagcgt ggacggcgac gccgatcacg acatcattgg attcggcaac gaagtgacca
     9661 ttcccgtcgt tgctagcggt ggttccggca acgacatcat cgacggcacg agcgagagcg
     9721 acatcctgaa tggggactcg ggcgacgatc acctggccgg tctgggcggc gacgatctcc
     9781 tgaacggcgg cccggacgac gacacgatct tcggcggcga cgatgacgat ctgatcgacg
     9841 gaggccaggg ggacgactcg ctggatggcg gcagaggaaa cgacaacatc gccggcgaca
     9901 ttcgatttgt gctcggtgag attggcggca atgacaccat cgtcggcgat tccgggaacg
     9961 acattatcga tggcgacggc ggcgatgaca gcctggatgc tggatcgggc gaagacactc
    10021 tcgatggcgg cgatggcaat gacgtcctgg tcggcggcag cgacgctgac tcgctcagcg
    10081 gcggcgacgg caatgaccag ttgctcggcg gcatgcggat cgccagcttg gatgcgctgg
    10141 aagccgaccc gagcgccgac acgctgagcg gcggcgccgg caacgacacg atcatcgccg
    10201 acgacggctc gttcctgccg ctgatcgtgc gggagacgat cggaggaaac gacgtcgtcc
    10261 gcggcggcag tggcaacgac ctgatcttca gcggctttgg caatgacaac gtgcagggtc
    10321 agagcggcga cgacacgatc gcggccggtc cgggcaacga cacggtaatc ggcggctggc
    10381 tgatttttgt gccatttcca ccgcttggtg acggcgacga cttcatcaac ggcggcgtcg
    10441 gcgctgacct gctgtatggc gataacttcg acccgctgct gccgccctac gtcagcttga
    10501 gcggcggcga tgatgaactg ctgggcggcg acggcgacga cacgatttac ggtcaggcgg
    10561 gccacgacaa cctcgatggc ggggcggacg acgacgcgct gcacggcggc gtggggaacg
    10621 acgagttgac aggcggtccg gggaccgatg ggctggtggg ggatgtcggg accgatctgc
    10681 tgttcggcaa ccagggcccc gatacgctcg atggcggggc cgataacgac gttctggtcg
    10741 gcggatccga cgacgacgag ctgcagggtg gatcaggcga cgacgtgctg accggcgagg
    10801 ccggcgacga cttgctggtg ggtggccccg ggttcgacac cctcgacggc ggcgcggatg
    10861 acgatgggct gatcggcggg aatctcgggg gcgcgggggt gcaagccgat ttctcccccg
    10921 attcactgaa cggcggcgac ggcgacgacg tcctcctggg cgacagcggc tccatcaccc
    10981 cgttcttcat tccggcgttc gacatcttcg gcggcggcga ctcaatccag ggcggccccg
    11041 gcaacgacat cagctacggt caggccggca acgacttcat cggcggcggg cccggcaatg
    11101 acatcatcgt ctgcggcgtt ggcaatgaca tcgccagcgg cgacgagggc agcgatatcg
    11161 tggtcggcga cgcgggcgac gacctgctgg ccgggggcga cgcgaacgac accctcacag
    11221 gcggcagcgg caacgacctt ctggtcggcg gcgtgttctc ggccggagcg atggtgattg
    11281 ccgaaaccgg cgacgatctg cttcaaggcg actcgggcga ggacattctg ttcggtgata
    11341 gctgggcgct gggcgacccg ctgaatctcg gcgtgagcgg cggtcacgac acgctgcgtg
    11401 gcggtccggg cagcgatctg gccgtgggcc agaccggcaa cgatttgttg ttcggcgacg
    11461 caggcgacga caccctgctg ggcggcgacg ggaacgaccg gatccgcggc ggcgacggcg
    11521 acgatcagct tggcggcggg gccggacacg acctgttgct cggcgaagtg ggccaagacg
    11581 cgctcgccgg cgcgggcggc cgcgatgtac tcatcggcgg gcagggcgcc gacacgctcg
    11641 acggcggcga cgatgacgac atcctgatcg ctggcacgac cgcgcacgac gcgaacgacg
    11701 cggcgttgaa cttgatcctg gccgagtgga cctcgctgcg tgactatgaa tctcgcgtga
    11761 aaaatctcaa gggcacgaac aaccccgagt tcgacaaccg gctcaacgca gaggttttcc
    11821 tcaagaagaa cggcaacagc aacaacggca acggcaacaa tggcaacggt aagggaaaca
    11881 atggcaacaa cggcaacatg cccacggtct tcgatgatga cgccagcgac gagctgacgg
    11941 gttcatccgg agatgattgg ttcctctatg aaccggcgct agacgagttg gtggattggg
    12001 cgggcggtga gaacaagaat tgacggcgat cgagtcttcg agaatggaga cggcgcaaac
    12061 tcagtcagcc cactccgacc aggaccggcc cgtgcgggtg tggaactcgc tggcgagttt
    12121 ggcgagcgcc gcggggtcga gccaatcgat gttgccccct gcggccagct tccgctgagt
    12181 ggccagttgc acccagaggt cgatcagggc ctggtcgtcg ggcagcggcg cgggcagatg
    12241 ccagagccgc ccggtgagag cgatgcggcc cgtccggtcc agcgcaaccg acatgagttc
    12301 tccgttgtgc cgcactggcg gacagagcgg caggcacgtg gtgacgtccc acagccgggc
    12361 cgaccagtcg gcgctgcctg tgagcaacgt gtggccgtcc gggctgaata cggcggcagt
    12421 gaccaggcct gcgtgccgca tcggttcgcc cacgagctgg ccggtgggga ctttccaaag
    12481 tcgcacggtg gcatcgtagc cgccggtcac cgccagctga gagtctggac tgaaggccac
    12541 cacaaatacc tcgccgccgt gcggcagcgg ttcgccaatc cgttggccgc tctccgcgtc
    12601 ccagaacttc acggtttcgt cgttgctggc gctggcgatc acccggccat tgggactcac
    12661 cgacaccatc cggacgcggc cgcgatgccc cgtggcttcc gtggacttcc agagcaactc
    12721 aaaactcttc gcatcccaga cgcgcactcc gttgtcgggg ctggcagatc cgacgacgac
    12781 gcgcgctgcg tcggggctga atgcgatgga gttggcaggg ccgctagccg actcaaaggc
    12841 gtgcaccaat tcgcccgttt caccgttcca aatccgcacg atcccggcgc tgtcgccggt
    12901 cgccagcacg cggccgtcgg ggctgaaaac ggccgccacg actgcccgtg accgatgatc
    12961 cagcgccggg ctgcttggtt cgccgctggc ggcatcccag agttgcgccg taccgtcgtt
    13021 gctcgcggtc acaagcacgg cggagtcccg ccgaaacgac acatgctgaa tggtgcccgc
    13081 gtgctgcatg gccgcgccga gg
//
LOCUS       DASRJB010000328         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_376433, whole genome shotgun sequence.
ACCESSION   DASRJB010000328 DASRJB010000000
VERSION     DASRJB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376433"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFB80_08795"
     CDS             <1..1260
                     /locus_tag="VFB80_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HZN33905.1"
                     /translation="EGRVTRPRLTAELLRSLMDVVRARFHIPAAMLARILAAADPVVT
                     CNDERLRFEAFSGCASTYARIDFMPEAVETPSFGRGTTNVDFHAPLIAALARVRDSDR
                     LSLAVGAKSVELKRNDEQIVEKKVALPVRWLKGFVEVQAYQPRLLPVHELTGVEALRF
                     LRSLPRMKTSKHAIYVVPAGRGLRLSQQAAKEGVRVGGLERLRVLEGLASQATTMRVY
                     GDEESGTSGWELDFGVARFHLVLSPDVWRGFSGEGQALTRLADKSWERALPKVQKALA
                     WQAVIDEEGLSRQTRLAPPQIRSALAALAARGLVGYDLASGTYFHRELPFDLALVDKL
                     QPRLVAAKKLLAAGGVKPAAESRVRGASDELEYLVAGSGALHRVRISEESSRCTCPWY
                     AKHQGQRGPCKHVLAVQMFADQDSPGD"
     gene            1275..>2805
                     /locus_tag="VFB80_08800"
     CDS             1275..>2805
                     /locus_tag="VFB80_08800"
                     /inference="COORDINATES: protein motif:HMM:NF040119.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HZN33906.1"
                     /translation="MPDRTRTEQLQAILDKGDEKACLKFFAKVTEAQRREVAKTAIAV
                     FKQSLKLVPSGKNTWGRDPRLPAAQIAVLSACSLSELKALGWQSHPDAKMTFQLLESR
                     RPEWLPQYAECLLEQSAFTWPLVRALVRRGLCPRPQHENYTLGMIRTLDSWSGDYHHV
                     DPYKPSLIANLRADKELLQTEIWELFRIEGQGDLSLASHDKYLQLRETNTKYCWNLVL
                     KELSRTGEISRDRLLDESLAALNRGFAQFRVAWFSQFHELLEPTVEERLARQDAYLVL
                     LASPIPPSVTFALDALEIIDSERPLPAAATIDALQPVLLARHKGTAKRAVQWLHKLAD
                     REPKCNPEAASAAAQALAHEAAEVQKAAIDVLEKHGSAADAGLRKQVAQAASGVAASL
                     RKRLQTWQSSGGAAAEPAKKDKARPAAATSLADLEKQAAALADRWVRLAGIRPMLAAA
                     KEGRIEIAALDFGPLDAPRLDPAAQLRPVADIDEWIDVCAHFLESPEEIDEGERVLEG
                     LS"
BASE COUNT          489 a          894 c          983 g          439 t
ORIGIN      
        1 gagggccgcg tgacgcggcc gcggctgacg gccgagctgc tgcggtcgct gatggacgtg
       61 gtgcgggcgc ggtttcacat ccccgcggcg atgctcgcca ggattctcgc cgcggccgat
      121 cccgtggtca cgtgcaacga cgagcggctg cggttcgagg cgttctcggg ctgtgcgagc
      181 acgtatgcgc ggatcgactt catgccggag gccgtcgaga cgccgagctt cggccgtggc
      241 acgaccaatg tcgatttcca cgcaccgctg atcgccgccc tggcccgcgt tcgcgacagc
      301 gaccgcctct cgctcgccgt cggggcgaag agcgtcgagc tgaagaggaa cgacgagcag
      361 atcgtcgaga agaaagtcgc actgcccgtc cgctggctga aaggcttcgt cgaggtccag
      421 gcatatcagc cgcgattgct gccggtgcac gagctgacgg gagtcgaagc gctgcggttc
      481 ctgcgcagtc tgccgcggat gaagacgagc aaacacgcga tctacgtcgt acccgcgggc
      541 cgcggcttgc ggctcagcca gcaggcggcg aaggagggcg tgcgggtcgg cgggctggag
      601 cggctgcggg tgctcgaagg gctggcgagc caggcgacca cgatgagggt ctacggcgac
      661 gaagaatccg gcacgagcgg ctgggagctc gacttcggcg tggcccgctt ccatctcgtg
      721 ctcagccccg acgtgtggcg cggtttttcc ggcgaggggc aggctctgac gcgactggcc
      781 gacaagtcgt gggagcgtgc gctgcccaag gtccaaaagg cgctggcctg gcaggcggtg
      841 atcgatgagg aagggctgtc gcggcagacc aggctggctc cgccgcagat tcgctcggct
      901 ctggcggcgc tcgcggcccg cgggctcgtc ggatacgacc tggccagcgg aacttatttc
      961 caccgcgagc tgccgttcga cctggcactg gtcgataagt tgcagccgcg gctggtggcg
     1021 gccaaaaagc tgctcgccgc gggcggtgtg aagccggccg ctgagtcacg agtgcggggc
     1081 gccagcgacg agcttgaata cctcgtcgcc ggcagcggcg ccctgcatcg cgtgcgcatc
     1141 tctgaagaga gcagccgttg cacgtgcccc tggtatgcga agcatcaggg ccagcgcggc
     1201 ccgtgcaaac acgtgctggc cgtgcagatg tttgccgatc aggactcgcc cggcgactaa
     1261 ccacgagcct tcccatgccc gaccggacgc gcaccgagca gcttcaggcc attctcgaca
     1321 agggcgacga aaaagcctgc ctgaagttct ttgccaaggt cacggaagcc cagcgccgcg
     1381 aggtcgccaa gacagcgatc gcggtcttca agcagtccct gaagcttgtt ccgagcggca
     1441 aaaacacctg ggggcgcgat ccgcgcctgc cggcggcgca aatcgcggtc ctgtcggcgt
     1501 gctcacttag cgaattgaag gctctcgggt ggcagagtca tccggacgcg aagatgacct
     1561 tccagttgct cgagtcccgc cggccggagt ggttgcctca atatgccgag tgcctcttgg
     1621 agcaaagcgc tttcacctgg cccctggtgc gggcgctcgt ccgccgcggt ttgtgtcccc
     1681 ggccccagca cgagaactac acgctgggca tgatccgcac gctcgattcc tggtcgggcg
     1741 attatcacca cgtcgatcct tacaagcctt cgctgattgc gaatctgcgg gcggataagg
     1801 agctcctgca aaccgaaatc tgggagctgt ttcgcatcga aggccaagga gatctctcgc
     1861 tcgcctcgca cgacaagtac ctgcaactgc gcgaaacgaa cacaaagtac tgctggaacc
     1921 tcgtgctcaa ggagctgtcc agaaccggcg agatctcgcg cgaccggctg ctggatgaaa
     1981 gcctcgcggc gctgaaccgg ggctttgcgc aattccgcgt ggcgtggttc tcgcagtttc
     2041 atgagctgct ggagccgacg gtcgaggagc ggctggcccg ccaggatgcg tatctcgtgc
     2101 tgctagccag cccgattcct ccgagcgtta cgttcgccct ggatgccttg gagatcatcg
     2161 acagcgagcg accgctgccg gcggcggcga cgatcgatgc gctgcagccg gtgctgctgg
     2221 cccggcacaa ggggacggca aaacgagccg tgcagtggtt gcacaagctg gccgatcgcg
     2281 agccgaaatg caatccggag gcggccagcg ccgcagccca ggccctggcc cacgaagcgg
     2341 ccgaggtgca aaaggcggcg atcgacgtgc tggaaaaaca cggctcggcg gccgatgccg
     2401 gcctgaggaa gcaagtcgcg caggcggcgt ccggagtggc cgcgtcgctg cggaagcggc
     2461 tgcaaacgtg gcaaagcagc ggcggagctg ccgccgaacc agcgaagaaa gacaaggccc
     2521 gcccggcagc cgccaccagc ctggcggatt tggaaaaaca ggcggctgcg ctcgccgatc
     2581 gttgggtcag gctggcgggc atccggccga tgctcgcggc tgctaaggaa ggccggatcg
     2641 agatcgccgc gctcgacttc ggcccgctcg acgccccgcg gctcgatccc gcggcgcagc
     2701 tgcggccagt ggccgacatc gacgagtgga tcgacgtctg cgcgcatttt ctcgagtcgc
     2761 cggaggaaat cgacgaaggc gagcgcgtgc tggagggcct tagcc
//
LOCUS       DASRJB010000329         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4042558, whole genome shotgun sequence.
ACCESSION   DASRJB010000329 DASRJB010000000
VERSION     DASRJB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042558"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFB80_08805"
     CDS             complement(<1..226)
                     /locus_tag="VFB80_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33907.1"
                     /translation="MTFGDQDRALLAQLADELIPASENMPSASQAGASGVHLDQVLAA
                     RPDLAAGLRDLLDQARGSECADFVSRLRQQN"
     gene            complement(223..1839)
                     /locus_tag="VFB80_08810"
     CDS             complement(223..1839)
                     /locus_tag="VFB80_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZN33908.1"
                     /translation="MNAAVKPDALIVGAGPTGAVAAKRLAEAGMRVVVLEQGGWPDYS
                     KARAGHADYEITAGRDWSANPNRRKAPADYPIDDADSDIAAVMYNAVGGSSVLFAAHW
                     QRNMPSDFRVRTLDGVGDDWPLTYEDLQPFYERVEGEFGVSGLAGDPAFPPGKGPPLP
                     PAPLGAMGQRVARAHNRLGWHWWPAPNAIATRPYGPLKQCTQRGTCMWGCVEGAKGSA
                     DRTHWPANVASGVLLVTGARVRRLEVGPGGLVTGATWIDREGREHFQAADVTILATNG
                     IGTPRLLLLSACNRFPDGLANSSGLVGRRLMMHPFGVVVGLFDEDLNSTHGTWGQCLH
                     SLEFYETDAQRGFVRGAKWGLQPTGGPVSMTRSYPWGDNAIWGAGFHEGIRKRLGHSA
                     MWGIIAEDLPDDANRVLLDPVLKDGDGIPAPKIAYRMSENSHNLMRFHLARAQESLEA
                     AGAYETVVAPQIRETGWHLLGTARMGSEAATSVVDSHGRSHDIPNLFIFDGSIWPTSS
                     GMNPTATIAAMSAWCSEHLVQNRTNQRVPA"
     gene            complement(1862..>2708)
                     /locus_tag="VFB80_08815"
     CDS             complement(1862..>2708)
                     /locus_tag="VFB80_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZN33909.1"
                     /translation="LLATADAMLASTERLSREVLAKTKLRAVARMGVGYDSVDVPAAT
                     DLGIAVTITPGTLEESVAEHTVALLLGVSRGLVTRDREVRAGRWMRLPMPRMAGRTFG
                     IIGLGRIGRAVVPKVQGLSMRVVAYDPFADRQVASRLGVTLMSLSELLQSADVVSLHT
                     TTTTDTQNLINRRTLAQMKPGSILINTGRGALVDEDALCEALDRGHLFGAALDVFKME
                     PLPLDSKLLAYPNVLLCTHMGGMDWDSQTAASNLAAQCIVDLYRGRWPEACVVNRELR
                     DRYKW"
BASE COUNT          400 a          957 c          929 g          422 t
ORIGIN      
        1 agttttgctg ccgcaggcgc gaaacgaagt cggcgcactc gctgccgcgg gcctggtcga
       61 gcaaatcccg caggccggcg gcgaggtcag gccgggccgc gagcacctga tccaggtgaa
      121 caccactggc gcccgcctga ctggcagacg gcatgttctc gctggccgga atcagctcgt
      181 cggccagttg cgcgagcaac gcacggtcct ggtcgccaaa ggtcatgcgg ggactcgctg
      241 gttagtccga ttctggacga ggtgctcgct gcaccaggcc gacatggcgg cgatcgtcgc
      301 cgtcggattc atgccgctgg aggtcggcca gatgctgccg tcgaaaatga acaggttggg
      361 aatgtcgtgc gatcggccgt ggctatcgac gaccgacgtg gccgcttcgc tgcccatcct
      421 ggccgtgccc aggaggtgcc agccggtctc gcgaatctgc ggcgcgacga cggtttcgta
      481 cgccccggcg gcctcgagcg attcctgggc ccgcgcgagg tgaaaccgca tcagattgtg
      541 cgaattctcc gacatgcgat aggcgatctt tggcgcgggg atgccgtcgc cgtccttgag
      601 caccggatcg agcagcacgc ggttggcgtc gtcgggaaga tcctcggcga tgatgcccca
      661 catggcgctg tggcccagcc gcttgcgaat tccttcgtgg aagcccgcgc cccagatcgc
      721 gttgtctccc cagggatagc tgcgggtcat cgagaccggc ccgcccgtcg gctgcaaacc
      781 ccacttggcc ccgcggacga agccgcgctg ggcatccgtc tcgtaaaact ccaggctgtg
      841 caggcactgg ccccaggtgc cgtgcgtgct gttgaggtcc tcgtcgaaca ggcccacgac
      901 gacgccgaag gggtgcatca tcagccggcg gccgacgagg cccgacgaat tcgccagacc
      961 atccgggaac cggttgcacg ccgacaagag cagcagccgc ggcgtgccga tgccgttggt
     1021 cgccaggata gtcacgtcgg ctgcttgaaa atgctcgcgg ccttcgcggt cgatccaggt
     1081 cgcgccggtg accagcccgc ccgggccgac ttccagccgg cggacccgcg cgccggtgac
     1141 cagcagcacg ccgctggcga cgttcgcggg ccagtgcgtg cgatcggccg agcccttggc
     1201 cccttcgacg cagccccaca tgcaggtccc gcgctgcgtg cactgtttca gcgggccgta
     1261 gggccgcgtc gcgatggcgt tcggcgcagg ccaccagtgc cagccgagac ggttatgcgc
     1321 gcgggccacg cgctggccca tcgcgcccag cggcgcgggc ggcagcggcg gtcccttgcc
     1381 cggcggaaag gccgggtcgc ccgccaggcc cgacacgccg aactcgccct cgacccgctc
     1441 atagaacggc tgcaggtcct cgtacgtcag cggccagtcg tcgccgacgc cgtcgagcgt
     1501 gcggacgcgg aagtccgagg gcatgttccg ctgccagtga gccgcgaaca gcacgctgct
     1561 cccgccgacc gcgttgtaca tcaccgcggc gatatcggag tcggcgtcgt cgatcggata
     1621 atcggccggg gccttgcgcc ggttggggtt cgccgaccag tcgcgtccgg cggtgatttc
     1681 gtagtcggcg tgccccgcgc gggccttcga atagtcaggc cagccgccct gctccagcac
     1741 gacgacgcgc attcccgctt cggccagccg cttcgccgcg actgcgccgg tcggcccggc
     1801 cccgacgatc agcgcgtccg gcttcacggc tgcgttcatg cacagactcc gctccgcccc
     1861 ctcaccactt ataccggtcg cgcagctcgc ggttgacgac gcaggcctcg ggccagcggc
     1921 cgcggtagag atcgacgatg cactgggcgg cgaggttgct ggcggcggtc tgcgaatccc
     1981 agtccatgcc ccccatgtgc gtgcagagca ggacgttggg atacgccagc agcttgctgt
     2041 ccagcggcag cggctccatc ttgaagacgt cgagcgcggc cccgaacagg tggccgcggt
     2101 cgagcgcctc gcagagggca tcttcatcga ccagggcccc gcggccggtg ttgatcagga
     2161 tcgagccggg cttcatctgg gccagcgtgc ggcgattgat caggttttgc gtgtcggtgg
     2221 tcgtcgtggt gtgcaggctg accacgtcgg ccgattgcag cagttccgac aggctcatca
     2281 gcgtcacgcc gaggcggctg gcaacctggc ggtcggcgaa cggatcgtag gcgacgaccc
     2341 gcatgctgag gccctgcacc ttcggcacga cggcccggcc gatgcggccc agcccgatga
     2401 tgccgaacgt gcggcccgcc atccgcggca tcggcagtcg catccagcgg ccggcgcgga
     2461 cctcgcggtc gcgcgtcacc agcccgcgcg acacgcccag gagcagcgcc accgtgtgtt
     2521 cggcgaccga ttcttccagc gttcccggcg tgatcgtcac ggcgatgccc agatcggtcg
     2581 ccgccggcac gtccaccgag tcgtagccca cgcccatccg tgcgaccgcc cgcagcttcg
     2641 tcttggccag cacctcgcgc gacagccgct cggtgctggc gagcattgca tcggccgtgg
     2701 ccaagagc
//
LOCUS       DASRJB010000330         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2632160, whole genome shotgun sequence.
ACCESSION   DASRJB010000330 DASRJB010000000
VERSION     DASRJB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632160"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3902
                     /locus_tag="VFB80_08820"
     CDS             <1..3902
                     /locus_tag="VFB80_08820"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN33910.1"
                     /translation="QKIEVVILVDTSASQAGKYRDGALAAVDSLLANLRSTDRVMLMA
                     VDTKAVPMSAGLVAAGSPEMRAGVAKLQGRAPLGATDLEAGLRSAASQFATADTARTV
                     IYVGDAMSKANVFSEASLGNLVGELRGKKVSVSGFVIGMQPNTQLLAALANHTGGMVA
                     LDGSAGDAPVTSGRALADSVHASVIWPSDMKMSVNVATAYPMLAPPLRSDRDSILVGT
                     LGQGGEVSVDVDGTLNGKPVATTFKATAERSNEDFAFLPKLVDIAASDKGLSLPTVGS
                     PGLRAAALTNLASAQQLASLGHEALSSGNVAGAQRAADAALARDPANPQAQALRSAAR
                     KTATGSEPALKLAGYSEAATQASSLLQEALAEQPGFLGGVEQERGVLAGKIRAEVEKA
                     LNDARQLMGADPEAAEQNLKLTLQNIESSPDLDAAVRSQLRQQVEDTIRQTHQQAILV
                     ADRLADAEQQKAIAIELARLNTDLVQRQQKIKQIMDRFDSLMEEGRYQLADEEITPEI
                     QRIAPYTPIESSVVFGGRMTRAAFENEIVWRARDNNSVRTLYQVELSLIPFPDEPPIV
                     YMPAEQWEDLTIRRQKYKAVDLGKQGGSEARIFDELNKVMPVGMEFVETPLKDAMQYL
                     QDQHNIPIVINTKTLNDAGVNIDAPVTKNLRGITLRSALRLLLKDLDLTYVVRDEVLQ
                     ITTPDDAESQLVTKVYPVGDLVVPIGINSNLFGLGGVGGMNGMGGMMGGMGGGMGGMG
                     GGMMGGGMGGMGGMGGMMGGGGMFAVEDDLKLSPKKAAADKVAAPPQPEVRRPAPAKA
                     KAARRIKLQPAAGESLAQAWDNYFAAQKDRVSKLDEQPAAAAVAESLAAVRETVRQLM
                     HEKKYAEVATVIQTALRYAHIESWMYESLALAMRADAAEKEQPLNYDELERALLSAVD
                     FAQSDNQVLMTAAYMNTIGLNERALKLYQQVGNTNPARPEPFIQGLEIAKRLDDLAAI
                     QWGCVGVLSHAWTAEHRSIGENAYRVGKATYEKLLAEGKKDQAEAFDAAVRAAQQRDC
                     LVTVTWTGDADIDVSVEEPSGTIVSQRQPRSAGGGVHLGDVSAAGGKNSVKGFSETYV
                     CPQGFDGQYRVHIRNIWGRPTSGKVTVEVVTHMGSKEQGSMREQIELTDKNALVTFDL
                     KHGRRKDSLPETQIAQVAKVQQAKNRMLIAQQLAQYEDSEASRGYAASVEEANRGGVG
                     PGGQGGAFRRRGVGYRPQITTLPEGANFSSNAVISADRRYVRVSPSPTFSQITEVNTF
                     NFVTGQGATQGAGQGGVGGVGGGVGGGQGGFGGGGFF"
BASE COUNT          776 a         1310 c         1379 g          626 t
ORIGIN      
        1 cccagaagat cgaagtcgtg atcctggtcg atacttccgc cagccaggca ggcaaatatc
       61 gcgacggagc gctggccgcc gtcgattcgc tgctggcgaa cctgcggtcc accgatcgcg
      121 tgatgctgat ggcggtcgat accaaggccg tgccgatgag tgcgggcttg gtggcggcgg
      181 gcagcccgga gatgcgggcg ggcgtcgcca agttgcaggg ccgcgctccg ctcggggcta
      241 cggacctgga agccggcctg cggtcggcag ccagccagtt tgcaacggcc gacacggccc
      301 gcacggtgat ttacgtcggc gacgccatga gcaaggcgaa cgtcttctcg gaagcttcgc
      361 tgggcaatct cgtgggcgag ctgcgcggca agaaagtctc ggtctccggc tttgtgatcg
      421 ggatgcagcc caatacgcaa ctgctcgcgg ccctggccaa ccacaccggc ggcatggtgg
      481 cgctcgacgg cagcgccggc gacgcccccg taaccagcgg ccgggcactg gccgactcgg
      541 tccatgccag cgtgatctgg ccgagcgaca tgaaaatgtc cgtcaacgtc gccacggctt
      601 acccgatgct cgcgccgccg ctgcggagcg accgtgactc gattctggtc ggcacgctgg
      661 gccagggcgg cgaagtgagc gtggatgtcg atggcacgct gaacggcaag ccggtggcca
      721 ctaccttcaa ggccacggcg gaacgctcga acgaggattt cgcatttctg ccgaagctgg
      781 tggatatcgc ggcctccgac aagggcctgt cgctgcccac ggtcggttcg cccggtctgc
      841 gggcggctgc cctcacgaac ctggccagcg cgcagcagct cgcaagtctg gggcacgaag
      901 ctctgagcag cggcaatgtc gccggcgctc agcgggcggc cgatgcggcc ctggcccgcg
      961 accccgccaa tccgcaggcc caagctctga ggagcgccgc tcgcaagacc gccactggaa
     1021 gtgagcccgc gctcaagctg gccggctata gcgaagccgc cacgcaggcc agcagcctgc
     1081 tccaggaggc cctcgccgag cagcccgggt tcctgggcgg cgtcgagcag gagcgcggcg
     1141 tgctggccgg aaaaatccgg gccgaagtcg aaaaggcgct caacgacgcc cggcagctga
     1201 tgggcgccga tcccgaggcc gccgagcaga acttgaagct cacgctgcag aatattgaaa
     1261 gctcgcccga tctggacgcc gcggtccgca gccagttgcg gcagcaagtg gaagacacca
     1321 tccgccagac ccaccagcaa gcgattctcg ttgccgaccg cctggccgac gccgagcagc
     1381 agaaagcgat cgcgattgag ctggcccgcc tgaataccga tcttgtgcaa cgccagcaga
     1441 agatcaagca gatcatggac cgcttcgatt cgctgatgga agagggccgc tatcagctgg
     1501 ccgacgagga aatcacgccc gagatccagc ggattgcccc gtataccccg atcgagtcgt
     1561 cggtagtgtt tggcggccgc atgacgcggg ccgcgttcga aaacgagatc gtgtggcgag
     1621 cccgcgataa caactctgtt cgcaccctgt atcaggtcga gctgtcgctc atcccgttcc
     1681 cggatgagcc gccgatcgtc tatatgcccg ccgagcagtg ggaagacctg acgatccgcc
     1741 gccagaagta caaggcggtc gatctgggca agcaaggcgg ctcggaagcc cggatcttcg
     1801 acgagttgaa caaggtcatg cccgtcggca tggaattcgt cgagacgccg ctcaaggacg
     1861 cgatgcagta cttgcaggac cagcacaaca ttccgatcgt catcaacacc aagaccctca
     1921 acgacgctgg cgtcaatatc gacgcccccg tgaccaagaa cctccggggc atcacgctgc
     1981 ggtcggccct gcggctgctg ctgaaggacc ttgacctgac ctacgtcgtc cgcgacgaag
     2041 tgctgcagat caccacgccc gacgacgccg aaagccagct ggtgaccaag gtgtatcccg
     2101 tcggcgacct ggtcgtgccg atcggcatca acagcaacct gttcggcctg ggcggtgtcg
     2161 gtggcatgaa cggcatgggt ggcatgatgg gcggaatggg cggtggaatg ggtggtatgg
     2221 gcggcggcat gatgggtggt ggcatgggtg gcatgggcgg catgggcggc atgatgggcg
     2281 gcggcggcat gttcgccgtc gaagacgacc tgaagctcag cccgaaaaag gccgccgcgg
     2341 acaaagtcgc cgcaccgccg cagcccgaag tccgccggcc ggcgccggcc aaggccaagg
     2401 ccgccaggcg gatcaaattg cagcccgcgg cgggcgagtc gctcgctcag gcgtgggaca
     2461 actacttcgc ggcccagaag gaccgcgttt ccaagctcga cgagcaacct gcggcggcgg
     2521 ccgtcgccga gtcgctggcc gcagtgcgcg agaccgtgcg gcagctgatg cacgaaaaga
     2581 agtacgccga agtcgcgacc gtgattcaga ccgccctgcg ctacgctcac atcgagtcgt
     2641 ggatgtacga atcgctggcc ctggcgatgc gggccgacgc tgcggaaaag gagcagccgc
     2701 tgaactacga cgagttggag cgggccctgc tctcggcggt tgacttcgcc cagagcgata
     2761 accaggtttt gatgactgcg gcctacatga acacgatcgg cctgaacgag cgggccttga
     2821 agctttatca gcaggtcggc aacaccaacc ctgcgcgtcc agagcccttc attcaaggat
     2881 tggagatcgc caagcggctc gacgatttgg cagcgatcca atggggctgc gtgggagtgc
     2941 tctcgcacgc ctggaccgcg gagcaccggt cgatcggcga aaacgcctac cgcgtcggca
     3001 aggcgaccta cgaaaagttg ctggccgaag gcaaaaagga ccaggccgag gcgtttgacg
     3061 ccgcggttcg ggccgcccag cagcgcgatt gcctggtcac ggtcacctgg accggcgacg
     3121 ccgatatcga cgtctcggtc gaggagccgt cgggcacgat cgtctcgcag cggcagccgc
     3181 gatcggccgg cggcggcgtc catctgggcg acgtctcggc ggctggcggc aagaactccg
     3241 tcaagggctt ttccgagacg tacgtctgcc cgcagggatt cgacgggcag tatcgcgtgc
     3301 acattcgcaa tatctggggc cggcccacca gcgggaaggt caccgtggaa gtcgtgaccc
     3361 acatgggctc caaggagcaa ggctcgatgc gcgagcagat cgagctgacc gacaagaacg
     3421 ccctcgtcac gttcgacctc aagcacggcc gccgcaagga ttcgctgccc gaaacccaga
     3481 tcgcccaggt ggccaaggtg cagcaggcca aaaaccggat gctgatcgcg cagcagctgg
     3541 cccagtacga agactccgag gcttcgcgcg gctacgcggc ttcggtcgag gaagccaatc
     3601 gcggcggcgt tgggccgggc ggacaaggcg gtgcattccg ccgccgcggc gtcggctatc
     3661 gtccgcaaat tacgacgcta cccgaaggcg cgaacttcag cagcaacgcg gtcatttcgg
     3721 ccgaccggcg ctacgtccgc gtctctccct cgccgacgtt ctcgcagatc accgaggtga
     3781 acacctttaa cttcgtcacc ggccagggcg ccactcaggg tgcaggccaa ggtggcgtcg
     3841 gtggcgtcgg tggcggcgtt ggcggcggcc aaggcggctt cggcggcggc gggttcttct
     3901 aatcctcgtc ggtcgtctgg gaacatttgc ggggccgctc cacatcgcgt ggacgcggcc
     3961 cttcttgttt agcgtgcgca tttcggccga cggcgaaacg gaacgcaaat tcgggtgcaa
     4021 actgagtcgg cgacagcgca tttgaagtta acacgggcgt catcgatcat gagtcaccgc
     4081 ccacggcatg t
//
LOCUS       DASRJB010000331         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3476384, whole genome shotgun sequence.
ACCESSION   DASRJB010000331 DASRJB010000000
VERSION     DASRJB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476384"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..2250)
                     /locus_tag="VFB80_08825"
     CDS             complement(337..2250)
                     /locus_tag="VFB80_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33911.1"
                     /translation="MPDWSYRTVFRPLLFRLPLATARDLCLGFMGQLARLPLGSFVID
                     FLGHMRPDPRLRTDLAGLTLAGPVVLGPQLDPRLLATRALARFGVGAIEIGPVALVDR
                     GQPAAATRRDDAQSLWLPEPLPAIGVETAVRRLAAARPAVPVIARIATDVEDVTSIVQ
                     QLAPNVAAFAVESPQHVAAIIPHKPALLCLPADGVGTQLDIPEGVSALLIDGRVRDPA
                     AGWLVGRAALPAAIEAVGRLRPEVGSALPIIAGGAVDPEGAMQLLAAGATAVQVDAGL
                     VFAGPGLPKRINDAVLFARNREAGEKAPESHELPGELHAAAMSWFWGLLLGVAMFGGG
                     LLALAIACTRVVLPYDEEYLGMSAAELTAKVNPRLLAFMAHDRVTLAGTMLAVGIQYL
                     FLAWFGICRGLHWAKMTLVASAFAGFGTFFLFLGFGYFDPFHAFVTAVLFQFLLLAFQ
                     SRLAPAEPPPWPNLVDDWRWKLAQWGQLLLLIEAAAVMAAGVTICTIGSTSVFVPEDL
                     EFMQTTVEQLRAANPRLVPVVAHDRASFGGMLLSVGIATLLPVLWGVRQGETWLWRML
                     ALSGSVGYVATLIVHLHVGYTSLDHLAPAVAGLALLWLGLGLLYPYLAPPAAEHRSRW
                     QALMRHWPGVVHS"
     gene            complement(2298..3125)
                     /locus_tag="VFB80_08830"
     CDS             complement(2298..3125)
                     /locus_tag="VFB80_08830"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HZN33912.1"
                     /translation="MPPEFSDACRLAAAAIAAADAILIGAGAGLGVDSGLPDFRGNEG
                     FWQAYPPFKKLGLSFVKLANPYWFRADPEQAWGFYGHRRNLYRRTQPHAGFAILRRWC
                     ERANWFVFTSNVDSHFQRAGFDAERIVECHGSLEFLQCQQFCSLEIWPADEAEVEIEL
                     ETFRARPPLPACPRCGGLARPNVLMFGDDEWLEQRTGAQHDRYLTWRRSLRGQRLVVI
                     ELGAGTHVPTIRLECESAAETLIRINPREPQAPAGAISLPLGALDALTQIDGCLDAG"
     gene            complement(3243..3326)
                     /locus_tag="VFB80_08835"
     tRNA            complement(3243..3326)
                     /locus_tag="VFB80_08835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3290..3292),aa:Leu,seq:cag)
     gene            complement(3378..3710)
                     /locus_tag="VFB80_08840"
     CDS             complement(3378..3710)
                     /locus_tag="VFB80_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN33913.1"
                     /translation="MSLPLEIDVRSVKQMRDAGQTFTLLDVREPNEIATAAIAGSVRI
                     PMRQVPARLAELGEKSGRIVVHCHHGGRSQRVTHYLRQQGFLQVQNMSGGIDAWSLEV
                     DPAVPRYE"
     gene            3832..5085
                     /locus_tag="VFB80_08845"
     CDS             3832..5085
                     /locus_tag="VFB80_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZN33914.1"
                     /translation="MPSIDPQLAREFAVEAVRQLRAAGHQALWAGGCVRDQLMGLAPK
                     DYDVATDAPPDRVREVFGQRRTLAVGAAFGVITVLGPKGAGQIEVATFRKDAAYSDGR
                     HPDSVTYSDAEHDAQRRDFTINGLFFDPLAGQVIDYVGGQQDLAQRVIRAIGDPLARI
                     AEDKLRMLRAVRFAARFDFALDERTLAAIRQQASELVIVSAERIAAELRLILTHANRS
                     RGVQLLADSDLLDVVLPEGRFLRTDPAAWRSMLEILAALRAPTSAMAFAALLRPLHIA
                     DAALELPRRVFERWKLSTDELAGIEKLLAEEPLIRAASQQPWPQLQRVLVAPRVEELL
                     GYCTAVARVLDGSTAEIDFCRLKLAQPPEVLSPPPLITGDDLKRLGLTPGPTFRTLLE
                     AVRDAQLEGRIKTMDEAIALVRRLP"
     gene            5150..>5623
                     /locus_tag="VFB80_08850"
     CDS             5150..>5623
                     /locus_tag="VFB80_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33915.1"
                     /translation="MLRACLLMLLLYAALFAGYYYWIGPQFDPPWVFVGVGVVALIVS
                     GCIGALINVRTHYRDWSLLSAARHNLPWIDGRWTAVCGEVHPVHEPLTAPFSGEECVL
                     VEYDVGRPQRPSNSSQQGTAGSDFAGFLMNPCVVRTGSGEVRLLGFPHLDGFAERT"
BASE COUNT          988 a         1920 c         1859 g          856 t
ORIGIN      
        1 caagccattg ctgagaaata ggttgcggca tatgcgcgcc ctcccgatta tgcgcattat
       61 gcgcgctggg gactggttgc tccattcagc gcatcccgga cgaatcggcc atgcgcgcat
      121 agcctcagga tttccttgca cagaggtttt cgtcgcaagt gcatacgtca tcaagggttg
      181 cggtcaaaat gcgctgcggc cgattatgcg cattatgcgc gctagcggct gagtgccggt
      241 cctgcgatta ccaataatca cttgccaaag aacactgccc gcagcatctg cggcgcggat
      301 taaccggcgg gaattgtaac tgttgtacac gtaaagtcaa ctatgtacga cccccggcca
      361 gtgcctcatc agcgcctgcc agcggctgcg gtgctcggcg gccggcgggg ccaggtacgg
      421 atagagcaaa ccgaggccga gccagagcag agccaggccg gcgactgccg gggccaggtg
      481 gtccaagctg gtatagccca catggaggtg gacgatgagc gtggcgacgt aaccgacgct
      541 gcccgacagg gcgagcatcc gccacagcca ggtctcgcct tgccgcacgc cccacagcac
      601 cggcaagagc gtcgcgatgc ccaccgagag cagcatgccg ccaaagctcg cccggtcgtg
      661 ggccacgacg ggcaccagcc gcggattggc cgcccgcaat tgctcgacgg tcgtctgcat
      721 gaactccagg tcttcgggca cgaagaccga cgtgctgccg atggtgcaaa tcgtcacacc
      781 ggcggccatc acggcggcgg cttcgatgag cagcaagagc tggccccatt gggccagctt
      841 ccaccgccag tcgtccacca ggttcggcca gggcggcggc tcggctggcg cgaggcggct
      901 ctgaaatgcc agcagcagga actgaaacag gaccgccgtc acgaaggcgt gaaacggatc
      961 gaagtagccg aagccgagaa acagaaagaa cgtgccgaag ccggcaaacg ccgaggcgac
     1021 gagcgtcatc ttcgcccagt gcaacccgcg gcagatgccg aaccaggcca agaagagata
     1081 ttgaatgccg accgcgagca tcgtgccggc cagggtgacg cggtcgtggg ccatgaacgc
     1141 cagcagccgc gggttcacct tggccgtcag ttccgccgcg gacatgccca ggtattcctc
     1201 atcgtagggc aggacgacgc gggtgcaggc gatcgccagg gcgagcagcc cgccgccgaa
     1261 catggccacc ccgagcagca gtccccagaa ccacgacatc gcggccgcgt gcagctcgcc
     1321 cggtaattcg tggctttcag gggccttctc gcccgcctcg cggttgcggg cgaacaggac
     1381 cgcatcgttg atccgcttgg gcaagcccgg cccggcaaag accaggccgg catcgacctg
     1441 caccgccgtc gcgcccgcag ccagcagctg catcgcaccc tcgggatcga ccgcgccgcc
     1501 ggcgatgatc ggcaatgcac ttcccacttc agggcgcagc cggcccacgg cttcaatcgc
     1561 ggccggtaat gcggcgcggc ctacgagcca tccggccgct gggtcgcgaa cgcggccgtc
     1621 gatcaaaagc gcgctgactc cctcaggaat atccagctgc gtgccaacgc cgtcggccgg
     1681 caggcagagc agcgccggct tgtgcggaat aatcgccgcg acgtgctgag gtgactcaac
     1741 ggcaaacgcc gcgacgttcg gagcgagttg ctgcacaatg gaggtgacat cctccacatc
     1801 cgtcgcaatc cgcgcaatga ccggcacggc cggacgcgca gccgctagcc ggcgcacggc
     1861 ggtctccact ccgatcgccg gcagcggctc gggcagccac agagattggg cgtcgtcgcg
     1921 gcgcgttgcg gccgccggct ggccccggtc caccagcgcc acggggccga tttcaattgc
     1981 gccgacgccg aaccgtgcca gcgcccgcgt cgccagcagc cgcggatcga gctgcggtcc
     2041 cagcacgacc ggtccggcca gtgtgagccc ggccaagtcg gtgcgcaagc gaggatctgg
     2101 gcgcatatgg cccaggaagt cgatcacaaa gctgcccagc ggcagccggg ccaattgccc
     2161 catgaagccc aggcacaggt cgcgggccgt cgccagtggc aagcgaaaga gcagcgggcg
     2221 gaagacggtg cgatacgacc aatcgggcat tccgggcctc cgccgacgca acactacccg
     2281 aaaagagccg gcccatctta acccgcgtcg aggcagccat cgatctgcgt caaagcatcc
     2341 agagcgccca gcggcagcga gatggctccg gcgggggcct gcggctcgcg ggggttgatg
     2401 cgaatgagcg tttccgcggc ggattcgcat tccaggcgga tcgtcggaac gtgcgtgcct
     2461 gcgccgagct cgatgaccac cagccgctgg ccgcgtagcg agcgccgcca ggtcaggtag
     2521 cggtcgtgct gagcgcctgt ccgctgctcg agccactcgt cgtcgccgaa catcagcacg
     2581 ttgggccgcg ccagcccgcc gcagcgcgga caggccggca gcggcggccg ggcccggaag
     2641 gtctccagct cgatttcgac ctccgcttcg tcggccggcc agatttccag cgagcagaat
     2701 tgctggcact gcaggaattc cagcgagccg tggcattcca cgatccgctc ggcgtcaaag
     2761 cccgcccgct gaaaatggga atcgacgttg ctcgtgaata cgaaccaatt ggcccgctcg
     2821 caccaccgcc gcaggatggc aaagcccgcg tgcggctgcg tgcggcgata gagattccgc
     2881 cgatggccgt agaagcccca ggcctgctcg gggtcggccc ggaaccagta cggattggcc
     2941 agcttgacga acgacagccc cagcttcttg aagggcggat aggcctgcca gaagccttcg
     3001 tttccgcgga agtcgggcag gccggaatcg acgcccaggc cggctccggc cccaatcaag
     3061 attgcatcgg ccgcggcaat cgcagcggcg gccaggcggc aagcatcaga aaactcgggc
     3121 ggcatagagt tccgccggcc cagcaagtag gccgcttctg ccggaagggg cctcatggga
     3181 gcgcagcgac cagacacttc gccgcgtcat cctccccggt cgggcaggcg ggacctacca
     3241 agtgcggctg agaggacttg aacctccacg accttacggc cactagaacc tgaatctagc
     3301 gcgtctgcca gttccgccac agccgcggga cctcttggtt ataggatagg acaccatttt
     3361 gcgacaaggg gttcgcgtca ctcgtaccgc ggcacggccg gatcgacctc cagcgaccag
     3421 gcgtcgatgc cgcccgacat gttttgaacc tgcaaaaagc cctgctgccg caggtagtgc
     3481 gtcacccgct ggctgcggcc gccgtgatgg cagtgcacga cgatccggcc cgatttctcg
     3541 cccagctcgg ccagccgggc cggcacctgc cgcatcggaa tccgcaccga gccggcgatc
     3601 gccgccgtgg cgatttcgtt gggctcgcgg acgtcgagca acgtgaacgt ctggcccgcg
     3661 tcgcgcatct gtttgacgga gcggacgtcg atttccaggg gcagggacat gggctgtcag
     3721 tggtcagtag tcagtagtca gtggtcagtt gatagaacat cgtagcggaa atcgaacctg
     3781 tgcccagcaa ctgacaactg acaactgaca actgacgcgc cataattcgc catgccctcg
     3841 atcgaccccc agctggcccg cgaattcgcc gtggaggctg tgcgccagct ccgcgcggca
     3901 ggccaccagg ccctgtgggc cggcggctgc gtgcgcgatc agcttatggg gctggcgccc
     3961 aaggactacg acgtggcgac cgacgcgccg ccggaccgcg tccgagaggt tttcggccag
     4021 cggcggaccc tcgccgtcgg cgcggcattc ggcgtcatca ccgtgctggg tccgaagggg
     4081 gcgggacaga ttgaggtggc gacgtttcgc aaggacgcgg cctacagcga cggccgccat
     4141 cccgactcgg tgacgtattc ggacgccgag cacgacgctc agcgccgcga ctttacgatc
     4201 aacggcctct ttttcgatcc gctcgccggc caggtgatcg actacgtcgg cggccagcag
     4261 gacctcgccc agcgcgtgat ccgcgcgatc ggcgacccgc tggcccgcat tgccgaagac
     4321 aagctgcgga tgctgcgcgc ggtccgtttt gccgcccggt tcgacttcgc cctcgacgag
     4381 cggacgctgg ccgccatccg gcagcaagcc agcgagctgg tcatcgtcag cgccgagcgg
     4441 atcgcggctg agttgcggct gatcctcacg cacgccaatc gcagccgggg agtgcagctc
     4501 ctggccgatt cggacctgct cgacgtggtg ctgcccgagg gccgcttcct gcggactgac
     4561 ccggcggcct ggcggtcgat gttggagatt ctggccgcgc tgagggcgcc cacgagcgcg
     4621 atggccttcg cggcgctgct ccgcccgctg cacatcgcgg atgcggcgct cgagctgccg
     4681 cggcgagtct ttgagcgctg gaagctctcg accgacgagc tggccggcat cgaaaaactg
     4741 ctcgccgagg agccgctgat ccgcgccgcc agccagcagc cgtggcccca gctccagcga
     4801 gtgctggtcg cgccgcgcgt ggaggagctg ctcggctact gcacggcggt cgcccgagtc
     4861 ctggacggca gcaccgccga gatcgacttt tgccggctga agctcgccca gccgccggag
     4921 gtcctgagcc cgccgccgct gatcaccggc gacgacttga agcgactcgg cttgacgccc
     4981 gggccgacgt ttcgcacgct gctcgaagcc gtccgcgacg cccagctcga aggccggatc
     5041 aaaacgatgg acgaagcgat cgccctggtt cgccggctgc catagtagtc gtctcgctcc
     5101 gcgagacgtt ttcctcacgc ggagcgtgag gactaccgaa aggaacgcca tgctccgcgc
     5161 ctgcctgctg atgctattgc tctacgcggc cctgttcgcc gggtactact actggatcgg
     5221 gccgcagttc gatccgccgt gggtctttgt cggcgtgggc gtggtcgcgc tgatcgtctc
     5281 gggctgcatc ggggcgctga tcaacgtccg cacgcactac cgcgactggt cgctgttgtc
     5341 ggccgcgcgg cacaacctgc cctggatcga cggccgctgg accgccgtct gcggcgaagt
     5401 ccatccggtc catgagccgc tcaccgcccc attcagcggc gaagagtgtg tgctggtcga
     5461 atacgacgtc ggccgcccgc agcgcccttc gaattccagc cagcagggca cggccggttc
     5521 cgactttgcc ggctttctga tgaacccctg cgtcgtgagg accggctcgg gcgaggtgcg
     5581 gctgctcggc tttccgcacc tggacggctt cgccgaacgg acc
//
LOCUS       DASRJB010000332         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_657449, whole genome shotgun sequence.
ACCESSION   DASRJB010000332 DASRJB010000000
VERSION     DASRJB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657449"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFB80_08855"
     CDS             complement(<1..514)
                     /locus_tag="VFB80_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33916.1"
                     /translation="MRRFFWLGVIAAIAGCRPPEQIAKYTVTKPELIDPTLTAVAPPA
                     VAPPQQLLAAMVQAGEQSWFFKLTGDPAVVEPSRTRFLDFVKSIKFSAGTEPRPTWTL
                     PADWEELPGNQIRLATLRFQSGGQPLEITVIPAGGTVLDNVNRWRKQVNLEPIPADEL
                     ASTTESFKVDG"
     gene            complement(686..1501)
                     /locus_tag="VFB80_08860"
     CDS             complement(686..1501)
                     /locus_tag="VFB80_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN33917.1"
                     /translation="MFRTYIVGGLLLAVAAGCSSPNAQLSLIQEDKEQLLATIKDQRE
                     TIRALKAEDASKEARLAQAEKELARTSGGTRLSARPADSPPVKAEQLPWRSPPAKATP
                     PTKKTSSTSKGSLPDLAAHDERIQVDRQSGTARLDADIPFAEGQAVITAEGKRQLDDV
                     ARLLKSDEYKDLRIMVAGYAEGRPANAPAAEGEKFNSSRQLAAARAQAVADYLDRHGI
                     AQERLGVSGLGSRGPLGGSGADRLAAAGSVQIFLLEPDASVVGWGTTGEPIRR"
     gene            complement(1581..2894)
                     /locus_tag="VFB80_08865"
     CDS             complement(1581..2894)
                     /locus_tag="VFB80_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN33918.1"
                     /translation="MAGKFVDLKEASAMIGVSPDDLIAMRSRGDIFGYRDGASWKFKL
                     EEVERVISERRTGGQGGDSKILSADDDEFENMISGLSSKILAEKAQEESESGSVLISE
                     QELGVSSTGQSTIIGKGRKPEEAAGDSDLRLADESGKDPLGTGSDKLLEAPGSKLDLA
                     SASDVLHGSDIHVAAGSGTGDMPKPRSGSGTGDMPLKPSSKLDLGDDLSLGDEADLEI
                     GSDSALDEEIKPKSGKGSDKGSGKGSDVTLGAGDSGINLKPSDSGLSLEQEPLDLGGS
                     GVESLELPEDDEVISLESEVADPDQATQLKADNEFLLSPGETLAEDESDSGSQVIALE
                     DSESFDQEAATMLKAEPGGALAADAFQPVGMEGVGAQPVYVQIPKVELPYSIWNVLSL
                     GLVLCLLTVSGMMMMDIMFNMWSFSGTTSVTTGVMDAFLSILNMG"
     gene            complement(2962..3324)
                     /locus_tag="VFB80_08870"
     CDS             complement(2962..3324)
                     /locus_tag="VFB80_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33919.1"
                     /translation="MRAAVGGLVLGLGLLLAVGLGFPEAQAQKQAGQLVSHQPGHFQQ
                     TGSPDLLALSAEAEGRQQITVIDPKQKVLAVYHVDRITGAISLRSVRNMQWDLLIEDF
                     NSANPTPREIRALTQQQR"
     gene            3650..>4320
                     /locus_tag="VFB80_08875"
     CDS             3650..>4320
                     /locus_tag="VFB80_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33920.1"
                     /translation="MKPEVIPQAEMDTRVKAAQVQLDKHAYEIIQHHFHDSTGCPFWL
                     QKKRELKFDPLKEVKTYADIQKFPLFEDEWLRGGPASRWVPKAYAGKPTYIFETGGTT
                     GIPKSRVVIEDHWIDYEKFSETLPDKYFPKGSNWLMLGPSGPRRLRLAVEHLAQFRGG
                     ICFCIDLDPRWVVKLIKKGWMEHLEAYKQHCIDQVLTILSAGHDIKCAFSTPKLLQAL
                     CESLE"
BASE COUNT          737 a         1389 c         1388 g          806 t
ORIGIN      
        1 ggccatccac cttaaaactc tcggtcgtgc tcgccagctc gtcggcggga atcggctcca
       61 ggttcacttg cttacgccag cggttgacgt tgtccagcac cgtgccgcca gcgggaatca
      121 cggtgatctc cagcggctgc ccgcccgact gaaaccgcaa ggtcgccagc cggatttgat
      181 tgccgggcag ttcttcccag tcggcgggca gcgtccaggt cggcctcggc tccgtgccgg
      241 cagagaactt gatcgacttc acaaagtcca gaaacctggt gcggctgggc tcaacaacgg
      301 ccggatcgcc ggtcagcttg aagaaccagc tttgctcgcc cgcctgaacc atggccgcca
      361 gcaactgctg cgggggggcg actgcgggag gggcgactgc ggtcagcgtc gggtcgatca
      421 actccggctt ggtgacggtg tacttggcaa tctgctccgg gggcctgcac cccgcgatgg
      481 cggcgatgac gccgagccag aaaaatcgtc gcaagtccaa tcgcggcaac aacatcgctc
      541 aaagctcgca gcagcgagtc gtacggcaaa ggccggcaaa aggcgggcgg cggggccggc
      601 caggaaggat cgcgccgcgg cagatcggtg cgactactgg attatagcgc ggcagggggc
      661 gagccggata gtcttaattg ccgcgctatc ggcgaatcgg ttcgccggta gtgccccagc
      721 cgacgacgct ggcgtctggt tccagcagga agatctgcac gctgccggct gcggccaggc
      781 gatcggcccc ggagccgccg agcgggccgc gcgagcccag tccgctgacg ccgagccgct
      841 cctgggcgat gccgtgccgg tcgagatagt cggcgacggc ctgggcccgg gcggcggcca
      901 gctgccgcga cgagttgaat ttctcgcctt cggcggctgg ggcgttggcg ggccggcctt
      961 cggcatagcc ggcgaccatg atccgcagat ccttgtattc gtcgcttttg agcagacggg
     1021 ccacgtcgtc gagttggcgt ttgccctcag ccgtgatgac cgcctggcct tccgcaaacg
     1081 ggatgtcggc atcgagccga gccgtgcccg attgccgatc gacctggatc cgctcgtcgt
     1141 gcgcggcgag gtcgggcagg gaacccttgg acgtcgagct ggttttcttg gtgggcggcg
     1201 tggccttggc cggcggcgag cgccagggca gctgttcggc tttcacgggc ggcgaatcgg
     1261 ccggacgggc tgagaggcgc gtgccgcccg aggtgcgagc cagctccttt tcggcctgag
     1321 ctagccgggc ttccttcgag gcatcttcgg cctttagcgc gcggattgtc tcgcgctggt
     1381 ccttgattgt cgccagcagc tgctccttgt cttcctggat cagcgacagt tgcgcgttgg
     1441 ggctggagca gccggcggcg acagccagta gcaggccgcc aacgatgtag gtgcgaaaca
     1501 tgattcgact ccgttcgaat cgctcgtggg gcaggcaatc ttgcctgccg tgacagacag
     1561 gaatgtctgc cccactcaca ctaacccata ttcaaaatgc tcaggaaggc gtccatgact
     1621 ccggtggtca cgctcgtcgt gccgctgaac gaccacatgt tgaacatgat atccatcatc
     1681 atcatgcccg aaacggtcag caggcagagc accagcccca gcgagagcac gttccagatc
     1741 gagtagggca gctcgacctt cggaatctgc acgtagaccg gctgcgcgcc gactccctcc
     1801 atgcccaccg gctggaaggc gtcggccgcc agggccccgc ccggctcggc cttgagcatc
     1861 gtcgccgctt cctggtcgaa cgactcggaa tcttcgaggg cgatgacctg cgagccgctg
     1921 tccgattcgt cctcggccag cgtttcgccg ggcgagagca ggaactcgtt gtcggccttg
     1981 agctgtgtcg cctgatcggg gtcggcgact tcggattcga gcgagatgac ctcgtcgtct
     2041 tcgggcaatt ccagcgattc gacgcccgag ccgcccaggt cgagcggctc ctgctcgagc
     2101 gacaggccgc tgtcggaggg cttgaggttg atgccgctgt cgcccgcgcc gagcgtgacg
     2161 tcgctgccct tgccgcttcc cttgtcgctg cccttgccgc tcttgggctt gatttcctcg
     2221 tcgagggcgc tgtcgctgcc gatttccagg tcggcttcgt cgccgaggct gaggtcgtcg
     2281 cccaggtcga gcttgctgct gggcttgagg ggcatgtcgc ccgtgccgct gcccgagcgg
     2341 ggcttgggca tgtcgccggt gccgctgccg gccgccacgt gaatgtccga accgtgcagc
     2401 acgtccgacg cactggccag atcgagcttg ctgccgggag cttcgagcag cttgtcgctg
     2461 ccggtgccga gcgggtcctt gcccgattcg tcggccagcc gcaggtcgct atcgccggcg
     2521 gcttcctcgg gcttgcggcc cttgccgatg atcgtgctct ggccggtcga ggaaacgccc
     2581 agctcttgct cgctgatcag caccgagccc gactcggatt cttcctgggc cttctcggcc
     2641 aggatcttcg acgagaggcc gctgatcatg ttctcgaatt cgtcgtcgtc ggccgagagg
     2701 attttcgagt cgcccccctg gccgccggtg cgccgctcgc tgatcacccg ctcgacttcc
     2761 tcgagcttga acttccagct cgcgccgtcg cgatagccaa aaatatcgcc gcgggaacgc
     2821 atcgcgatca ggtcgtcggg cgagacgccg atcatcgccg aggcttcttt gagatcgaca
     2881 aatttgccgg ccatgcgact gccttctctc cagcggccgg caatgctccc ggcacgctcc
     2941 ttcgtgccaa gcgacgcctg cctaccgctg ttgctgggtt aacgcgcgaa tctcgcgcgg
     3001 cgtgggattg gcggagttga aatcctcaat gagcaggtcc cactgcatgt tgcggacgct
     3061 ccgcagcgag atcgctcccg tgatccgatc gacgtgataa accgccagca ccttctgttt
     3121 cgggtcgatg acggtaattt gttgccggcc ctccgcctcg gcgctcaaag ccagcaaatc
     3181 gggcgagccc gtttgctgga agtgccccgg ctgatggctg accaattggc cggcctgctt
     3241 ctgcgcctgg gcttcgggaa atcccaaacc cacggccaaa agcagaccca gccccaagac
     3301 aagaccgccg accgctgccc gcatggcaaa actccacgtg gctgctgaaa gcggaatccg
     3361 ggcttagacc attcattcta aaagcccgta aatccgtttc aagacttcga tttacgacaa
     3421 ttcgcccgcc cagccatcgc tggcgagcgg gaaggggccg gagtttagct atcgctctcc
     3481 tcggcggcaa ggcaaaccag cccggtgcag ccgttgattc aaacgcggcc agcggctatc
     3541 gtgagacgtt tgtttggaca agtgtttgcg tgcggcgcgt gaaacgagac aatagcacca
     3601 tgactgctgt tgaactcgga actctgaact cggaactctg aattctccga tgaagcccga
     3661 agtcatcccc caggcggaaa tggatacccg cgtcaaggcg gcccaggtcc agctcgacaa
     3721 gcacgcctac gagatcatcc agcaccactt tcacgacagc accggctgcc cgttctggct
     3781 gcagaagaag cgggagctga agttcgatcc gctcaaggaa gtgaaaacct acgcggacat
     3841 tcagaagttt ccgctctttg aggacgagtg gctccgcggc gggccggcca gccgctgggt
     3901 gcccaaagcc tacgcgggca agccgaccta catcttcgag acgggcggca cgacgggcat
     3961 tcccaaaagc cgcgtggtga tcgaggacca ctggatcgac tacgagaagt tctccgaaac
     4021 gttgcccgac aagtacttcc ccaaagggtc caattggctg atgctggggc cgagcggccc
     4081 gcggcgattg cggctggcgg tcgagcactt ggcgcagttc cgcggcggca tctgcttttg
     4141 catcgacctc gatccgcggt gggtcgtgaa gctgatcaag aagggctgga tggagcacct
     4201 ggaggcctac aagcagcact gcatcgatca ggtgctgaca atcctctcgg ccgggcacga
     4261 catcaagtgc gcattttcca cgccgaagct gctgcaagcg ctgtgcgaat cgctggagaa
//
LOCUS       DASRJB010000333         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3760174, whole genome shotgun sequence.
ACCESSION   DASRJB010000333 DASRJB010000000
VERSION     DASRJB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3760174"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFB80_08880"
     CDS             <1..326
                     /locus_tag="VFB80_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33921.1"
                     /translation="DYLLSNIIDPSAVVNKDYRMSIVRHADGRILNGLVTSQDEQRIV
                     LDTAKEKLTLLKADIDEIKLTTLSPMPDAILQPLKNEQVRDLIAYLMSAGQVELPEGF
                     AAEGR"
     gene            371..1672
                     /locus_tag="VFB80_08885"
     CDS             371..1672
                     /locus_tag="VFB80_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZN33922.1"
                     /translation="MNWPKFPPDLPLVEFAIVFLIGALVGIDRERRKAQEQQDEIGGL
                     RTFILLAEAGGVAAWLALRLDSLWIFAAAALVVGALIIAGYFASTRGREAALGQTTEV
                     AAVVVFFLGGLVIFGFRDLAVVLAIVTSAVLAYKHPLHGLVLNLSEDDVYAGLKLLIA
                     TFIVLPILPNQPLDPWGALNPYKMWWLVILISGLSLAGYVAARWLGSQHGLALTGLFG
                     GLASSTAVTLSFARRSSDRNAPAGMAEALAAGLLLAWTVMFGRIAVEVAVVHRPLLPV
                     LAVPLASMGIAAALAAGVFYWRSRSADQQPAENVPLKNPFSLTSAARFALLFTVVELA
                     VKLVQTYWPGRGFYAVAALAGLTDVDAITLSMAQYAKDGGSPQDAAVSIAIAALTNTL
                     VKLGLVAALGAAGLRWRTIAATVLIVACGGAAVYFLFLGAN"
     gene            1774..2049
                     /locus_tag="VFB80_08890"
                     /pseudo
     CDS             1774..2049
                     /locus_tag="VFB80_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
     gene            2076..3698
                     /locus_tag="VFB80_08895"
     CDS             2076..3698
                     /locus_tag="VFB80_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HZN33923.1"
                     /translation="MLILLALFSVARRLPAADEAAVNRFGLFEVELQAQGACANPYTD
                     LTGEVTITPPEGRARSALLFWDGRDQWKLRVSPDLPGTWKWSVKSRDAGLDGKSGSFQ
                     CVAAQRKGSIRPMAGHPLHFERQDGTPFWFLGDTAWALFTDSPAEKLDRAAALAYLDA
                     RAGQGFNVVHFMLLSEAGWGNQGGQPFDDLAAEKLNPGYWQEADLRLAHANSRGIVCG
                     LALAWGDKGRGEPYAWRKFRDAEARRRYARYIAARYSAFDTYFLLSGEWHAEIRSRQS
                     DEETVRQEFIDLGNVLHQADPHRRMIGIHPMTSQGSVREFARAEWMAFGDYQQNYVDL
                     HGRILQSRARNKPVVNAEYGYLLRDQSGDGVPDKDNSTSLAAMRHATWDIAMAGGYLV
                     TGFGTTYFGGNRDPGPFDLAAAKNKPWEEQVGRVKSIFGELPWPQLAPHDELLACPTP
                     RGAEGKEFGRTIPPPTAYWCLADPGKSYLVYFRGLKTPLTLTLPKDTPLPATQINPRT
                     GERLALGRAIDRFEIRPPDEQDWVIVLAQSAN"
     gene            complement(3710..>4819)
                     /locus_tag="VFB80_08900"
     CDS             complement(3710..>4819)
                     /locus_tag="VFB80_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN33924.1"
                     /translation="AELASFFAYQESFAGGVFVAAGDINGDGRADVITGADADPQATG
                     GHVKVFDGATGAEVRSFFAFPGFSGGVRVAAGDLNGDGLADIITGAGPGAGPHVKVFD
                     GLTHAELRSFFAFDPSFSGGVFVAAGDLNNSRPPGRLDLDVEIQTGLGDDRVLSDVVS
                     FEVQVLRRVRIDTGAGADRVNVIWAKLQNKTEFPSAAVDDFDLDMGAGDDVVRFQSDV
                     AADELQQKVRIDGGAGADQITVLLRRLANPHLPQDAVRASISPELTQIDIDGGSGNDR
                     ISLDFGGAILRQQAVDAVLRGGAGDDQVRARFDFAPGSTGRLSASVLGEAGNDELGLA
                     VLNAGDLDLLFALLDGGPGRDRCRTTANVVVKNCP"
BASE COUNT          774 a         1672 c         1576 g          797 t
ORIGIN      
        1 tcgattacct gctcagcaac atcatcgacc ccagcgcggt ggtcaacaaa gactatcgca
       61 tgtcgatcgt ccgacatgcc gacggccgca tcctgaacgg cctcgtcacc agccaggacg
      121 agcagcgaat cgtgctcgac accgccaagg aaaagctcac gctgctcaag gccgacatcg
      181 acgagatcaa gctcacgacg ctctctccga tgcccgacgc catcttgcag ccgctgaaga
      241 acgagcaggt tcgcgacctg atcgcctacc tgatgagcgc cgggcaggtc gagctgcccg
      301 agggattcgc cgccgaaggt cgctgacttc cgcggcggcg aactatgatg gcccgaggga
      361 gcgcaagcgc gtgaactggc cgaaattccc gccggacctg ccgctggtcg agtttgcgat
      421 cgtgttttta atcggggcgc tcgtcgggat cgaccgcgag cggcgaaagg cccaggagca
      481 acaggacgag atcggcggct tgcggacgtt catcctgctg gccgaagcgg gcggcgtggc
      541 cgcctggctg gcgctgcggc tggattcgct gtggatcttt gcggccgcgg ccctggtcgt
      601 cggggcgctt atcatcgccg gctatttcgc ctccacgcgg ggccgcgaag ccgccctcgg
      661 ccagacgacc gaagtggccg ccgtggtcgt cttctttctc gggggcctgg tgattttcgg
      721 ctttcgcgac ctggccgtgg tgctggcgat cgtcacctcc gccgtgctgg cctacaagca
      781 cccgctgcac ggcctggtgt tgaacctcag cgaggacgac gtctatgccg ggctcaagct
      841 cctgatcgcg acgttcatcg tcctgccgat cctgcccaat cagccgctgg atccgtgggg
      901 cgccctcaat ccttacaaga tgtggtggct ggtgatcttg atctccgggc tctcgctggc
      961 gggctacgtc gccgcgcggt ggctcggctc gcagcacggc ctagcgctga ccgggctctt
     1021 tggcgggctg gcttcgagca cggcggtgac gctgtcgttc gctcggcgga gcagcgaccg
     1081 gaatgctccg gccggcatgg ccgaagccct ggcggccgga ttattgctcg cctggaccgt
     1141 catgttcggc cgcatcgccg tggaggtcgc agttgtccac cggccgcttt tgccggtcct
     1201 cgccgtcccg ctggcgtcga tgggaatcgc cgcggcgctg gcggccggag tcttctattg
     1261 gcggagccgc tcggccgacc agcagccggc cgagaacgtg ccgctcaaga acccgttcag
     1321 cctgacctcc gcggcgcggt tcgccttgct cttcacggtg gtcgagctgg cggtcaagct
     1381 cgtgcagacg tactggccgg gtcgcggctt ctacgccgtc gccgcgctgg ccggactgac
     1441 cgacgtggac gcgatcacgc tctcgatggc ccagtacgcc aaggacggcg gcagcccgca
     1501 ggacgccgcc gtctcaatcg ccatcgccgc cctcactaac acgctcgtca agctgggcct
     1561 cgtcgccgcc ctgggcgccg ccggtctgag atggcggacc atcgcggcca ccgtgctgat
     1621 cgtggcctgc ggcggggccg cggtctattt cctgttcctg ggcgcgaact agcttccgcc
     1681 ggagagccaa aagccgtaag ttgcatggct gctatgtgcc gcctcccgcc ccgacgattc
     1741 gcgtggttcg tcctgctggc aatcgcgccg ccgctgcctg cctcgcaggg cggcgacggc
     1801 cgcgaccaca acccccgcgg cttcctggtc tggatggccg gcgcgggaat caagggcggc
     1861 gtcagctacg gcgagaccga cgagatcggc taccgcgccg ccgtcgatcc tgtcaccgtg
     1921 cacgacctgc acgccacgct gctgcacctg ctcggcatcg atcacaaacg attcacctat
     1981 ctgcacaacg gccggcgcta ccgcctgacc gatgtggccg gaaatctgct cacgaaaatc
     2041 ctggcgtagt cctccacatg gctcgagcgc tcagtgtgct gattctgctt gccttgttct
     2101 cggtggcgag gcgactgccg gcggcggacg aagctgcggt gaaccggttc ggactctttg
     2161 aagtcgagct tcaggcgcag ggcgcttgcg ccaatcccta cacggacctg accggcgaag
     2221 tcacgatcac gccgccggaa ggccgggcca ggtcggcgct gctcttctgg gatggccgcg
     2281 accagtggaa gctccgcgtt tcgcccgact tgcccgggac ctggaaatgg agcgtcaaga
     2341 gccgcgatgc ggggctggac ggcaaatcgg gaagcttcca gtgtgtggcc gcccagcgga
     2401 aggggagcat ccggccgatg gcgggccacc cgctgcactt cgagcggcag gacggcacgc
     2461 ccttctggtt cctgggcgac acggcctggg ccctcttcac cgacagcccg gccgagaagc
     2521 tcgaccgcgc cgccgcgctg gcctatctcg acgcccgcgc tggccaagga ttcaacgtcg
     2581 ttcacttcat gctgctcagc gaagccggct gggggaatca aggcggccag ccgttcgacg
     2641 acctcgctgc ggagaagctc aatccgggtt attggcagga ggcggacctg cggctggcgc
     2701 acgccaacag ccgggggatc gtttgcggcc tggcgctggc ctggggcgac aaggggcggg
     2761 gcgagccgta tgcctggcgg aagttccggg acgccgaggc ccgccggcgg tacgcgcggt
     2821 acatcgccgc gcggtacagc gctttcgaca cgtactttct cctctccggc gaatggcatg
     2881 cggaaatccg ctcgcggcag agcgacgaag aaaccgtgcg gcaggaattc atcgacctgg
     2941 gcaatgtcct gcaccaggcc gatccgcacc ggcggatgat cggcatccac ccgatgacca
     3001 gccagggcag cgtccgcgag tttgcccgcg ccgagtggat ggcgttcggc gactaccagc
     3061 agaactacgt cgatctgcac ggccgcatcc tgcagtcgcg cgcccgcaat aagcccgtcg
     3121 tgaacgcgga gtacggctac ctccttcgcg atcagagcgg cgacggcgtg cccgacaagg
     3181 acaacagcac cagcctggcc gcgatgcggc acgccacgtg ggacatcgcc atggccggcg
     3241 gctacctggt gaccggcttt ggcacgacct atttcggcgg caaccgcgat ccggggccgt
     3301 tcgacctggc ggccgccaag aataagccct gggaagaaca ggtcggccgc gtcaaatcga
     3361 ttttcggcga gctcccctgg ccgcagctcg cgccgcacga cgagctgctg gcctgcccca
     3421 cgccgcgtgg cgccgaggga aaggaattcg gccgcacgat cccgccgccg accgcgtatt
     3481 ggtgcctagc cgatccggga aagagctacc tcgtttattt ccgcggactg aaaacgccgc
     3541 tcacgctgac gctgccgaag gacacgccgc tgcccgcgac gcaaatcaat cctcgcaccg
     3601 gcgagcgcct cgccctgggc cgcgccatcg accgcttcga aatccgcccg cccgacgagc
     3661 aggattgggt catcgtgctc gcccagagcg ccaattgaac gccgaccgct cacgggcaat
     3721 tcttgacgac cacattggcc gtcgtgcggc agcggtcgcg gccgggtccg ccgtcgagca
     3781 gagcgaagag caggtcgagg tcgccggcgt tcagcacggc cagtcccagc tcatcgttgc
     3841 cggcctcgcc caggacgctc gccgagagtc gtcccgtgct ccccggagcg aagtcgaagc
     3901 gggcccgcac ctggtcgtcg cccgcgccgc cgcgcaagac tgcgtccacc gcctgctgcc
     3961 ggagaatcgc gccgccgaaa tcgagcgaaa tgcggtcgtt cccgctgccg ccatcgatgt
     4021 cgatttgcgt cagctcgggc gaaattgacg ctctgaccgc atcctgcggc aagtgcgggt
     4081 tcgccaatct ccgcagcaac acggtgattt ggtccgcgcc ggccccgccg tcgattcgca
     4141 ccttttgctg gagctcatcc gcggcgacgt cggactggaa ccgcaccaca tcgtccccgg
     4201 cgcccatgtc gaggtcgaag tcgtccacgg ctgcactcgg aaactcggtc ttgttctgca
     4261 gttttgccca gatgacgttg actcggtcgg cgccggcccc ggtgtcgatc cgcactctgc
     4321 ggagcacttg cacctcgaac gacacgacat cggatagcac ccgatcgtcg cccaggccgg
     4381 tctggatctc cacgtcgagg tcgagccgcc ccggcgggcg actgttattc aagtcgccgg
     4441 cagcgacgaa gacgcctccg gaaaagctcg gatcgaaggc gaaaaagctg cgcagctcgg
     4501 cgtgcgtaag gccatcgaag accttgacgt gcggccctgc gcccggcccc gcccctgtga
     4561 taatgtcggc aagcccgtcg ccattcaggt cgccggctgc gacgcggacg ccgcccgaga
     4621 agcctggaaa cgcgaagaaa ctgcgcactt ctgcgccggt cgctccgtca aagaccttga
     4681 cgtggccgcc ggtcgcctgc ggatcggcat ctgcgcccgt gatgacgtcg gcccggccgt
     4741 cgccgttgat gtcgcccgct gccacgaaga cgccgccggc aaaactctct tgataggcga
     4801 agaagctcgc cagctcggc
//
LOCUS       DASRJB010000334         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_946282, whole genome shotgun sequence.
ACCESSION   DASRJB010000334 DASRJB010000000
VERSION     DASRJB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946282"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..>2373
                     /locus_tag="VFB80_08905"
     CDS             167..>2373
                     /locus_tag="VFB80_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZN33925.1"
                     /translation="MPAILFELLKLLVIDLLFVAILLVLIAPLMQWKKAAWAVLKRNF
                     VGYFANPTGYVFLCLFVFLTSVAAFWPHDFFIANLANLDQLNKYIAMILLVFVPAITM
                     SIWADERRQGTDELLLTLPAADFDIVIGKYLAAASIFTVSLLFSQFSNYSILVSLASD
                     SEQGIVDVDTGLFFATYFGYWLMGMAMLSLGMVASFLTRNLTVSFILGMLFNAIPVVT
                     YYADVLVPASATARQVSQWSFASQFDDFGRGVISLSAAAFFVLIVVLGIYLSMVLIGK
                     RHWMRHDANPRPAGQQALAVLAAILAVPGLVALALAPILVGTASNVALAIGLIGAAAG
                     LGLYWTAGSMANPPHTGSMGLHFTLRALTIVVLLVGINSLLKRFDYSWLRYDATQGKI
                     SSLSPDTGKLLREIAQKQERPIFVDAYISAEMPEEYVKTRYNLVSMLKELQRQSGGKV
                     RLNLRENLSLFSEDAATAEQRFGIAKQTMQSTARGAFKEQQFIMGVAFTCGLEKVVLP
                     SFKLGMPVEYELVRSIATVAGEQRKKLGVVNTGAQMMGGFTMAGMQPRNIPKQLILDE
                     LERQYKVEDVDPTNPIEPGRYDVLLIVQPSSLGPPQLANVVEAIKKGQAAAIFEDPFP
                     MVMPQTVGTSLPNPPQGGMFGMGGQSAPKGDINALWKALDIQVTGFESDALPAPGQVV
                     WQSYNPYPKFAGRELSPELVFVRDDMPDNQEPFNRTEPVVSHFEEVLFPYPTG"
BASE COUNT          397 a          770 c          753 g          453 t
ORIGIN      
        1 gatccacgac ggccggctgg tgttcgacgg ctcgaccgga gacctgaccc acaagtacgg
       61 cagcctcgac aaggcgtttc actcgttgac ggcggccacg gccgcggtcc attagccggc
      121 gactgctccg ccccttcccg actctcccgt ttttccgata aacgccatgc ccgcgatcct
      181 gtttgagctc ttgaagctgc tggtgatcga cctgctcttc gtggcgatcc tgctggtgct
      241 gatcgcgccg ctgatgcagt ggaagaaggc cgcctgggca gtgctgaagc ggaacttcgt
      301 gggctatttc gccaacccga cgggctatgt gtttctgtgc ctgttcgtgt tcctgacgag
      361 cgttgcggcg ttctggccgc acgacttctt catcgccaac ctggccaacc tcgaccagct
      421 caacaagtac atcgcgatga tcctgctggt gttcgtgccg gcgatcacga tgagcatctg
      481 ggccgacgag cggcggcagg ggaccgacga gctcttgctc acgctgccgg ccgccgactt
      541 cgacatcgtg atcggcaaat acctggcggc ggcgtcgatc tttacggtct cgctgctctt
      601 ctcgcagttc tcgaactact cgatcctggt ctcgctggcc agcgactccg agcaggggat
      661 cgtggacgtc gatacgggcc tctttttcgc cacctatttc ggctactggc tgatgggcat
      721 ggcgatgctg tcgctgggca tggtggcctc gttcctgacc cggaacctga ccgtcagctt
      781 catcctgggc atgctcttca acgcgattcc cgtggtgacc tattacgccg acgtcctcgt
      841 gccggccagc gcgacggcgc ggcaggtttc gcagtggagc ttcgcctcgc agttcgacga
      901 cttcggccgc ggcgtaatca gcctgtcggc cgcggcgttc tttgtgctga tcgtggtgct
      961 ggggatttac ctgagcatgg tgctgatcgg caagcggcac tggatgcggc acgacgccaa
     1021 tccgaggccc gccggccagc aggccctggc ggttctcgcc gcgatcctgg ccgtgccggg
     1081 cctcgtggcc ctggcgctcg cgccgatcct ggtgggaacg gcgagcaacg tggcgctggc
     1141 gatcggcctg atcggcgcgg ccgctggcct ggggctctat tggaccgccg gttcgatggc
     1201 caacccgccg cacaccggtt cgatgggcct gcacttcacc ctgcgagcgc tgacgatcgt
     1261 cgtgctgctg gtcggcatca actcgctgct caagcgtttt gactattcct ggctgcggta
     1321 cgacgccacg cagggcaaga tcagctcgct ctcgcccgac acgggcaagc tgctccgcga
     1381 aatcgcccag aagcaggagc ggccgatctt cgtcgatgcc tacatctcgg ccgagatgcc
     1441 tgaggaatac gtcaagaccc gctacaacct ggtgtcgatg ctcaaggagc tccagcggca
     1501 atcggggggc aaggtccgcc tgaacctgcg cgaaaacttg tcgctgttca gcgaggatgc
     1561 cgctacggcc gagcagcggt tcggcatcgc caagcagacg atgcaatcca ccgctcgcgg
     1621 agcattcaaa gagcagcagt tcatcatggg cgtggccttc acctgcggcc tggaaaaggt
     1681 cgtcctgcct tcgttcaagc tgggcatgcc cgtcgaatac gagctcgtcc gctcgatcgc
     1741 caccgtcgcc ggcgagcagc gcaagaagct cggcgtggtc aacaccggcg cgcagatgat
     1801 gggcggcttc accatggcgg gcatgcagcc gcggaacatt cccaaacagc tgatcctgga
     1861 cgaattggag cggcagtaca aagtcgagga tgtcgatccg acgaacccga tcgagccggg
     1921 gcggtacgac gtgctcctga tcgtgcagcc gtcgtcgcta gggcccccac agctggccaa
     1981 cgtggtcgag gcgatcaaga aagggcaggc ggccgccatc tttgaagatc ccttcccgat
     2041 ggtgatgccg cagacggtgg gcacgagcct gcccaatccg ccgcaggggg gcatgttcgg
     2101 catgggcggc cagtcggcgc ccaagggtga cattaacgcc ctctggaagg ccctcgacat
     2161 ccaggtcacc ggcttcgaga gcgacgccct gcccgcgccg ggacaggtcg tgtggcagag
     2221 ctacaatccc tatcccaagt tcgcgggccg cgagctaagc cccgagctgg tctttgtccg
     2281 cgacgacatg ccggacaatc aggagccgtt caaccgcacc gagccggtgg tgtcgcactt
     2341 cgaggaagtg ctgttcccat atccgacggg cat
//
LOCUS       DASRJB010000335         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1787767, whole genome shotgun sequence.
ACCESSION   DASRJB010000335 DASRJB010000000
VERSION     DASRJB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787767"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..1489)
                     /locus_tag="VFB80_08910"
     CDS             complement(425..1489)
                     /locus_tag="VFB80_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZN33926.1"
                     /translation="MAIEFHCTQCGQMLRVADSSAGKGARCPGCKTVMVVPAAGDLAS
                     RSLPPLNQPAISQPVAPAAPPPAPQQPAAPGQDDLFAYLKQAVTPNAPPPAAYQQPGA
                     YQQPAAAPFADKPANPFGDQAAASANPYASPAPVAYSAYPSQPGELGLPWETKPPGLG
                     VWWETSQMCLSDAESAFRRMRQYGGIGSPMLYAVFGLLLGFVGQTAWSLPAMMFEAFA
                     GPAANPAGGQVAGMEVMVQLLLNGLITVVWATGGLLISAGVTHLCLMLVGGAHQGYET
                     TYRVTAFTAGSIAWLSVVPCFGPLIALVMTLICQIQGLAQAHETTAGRAAAAVLLPVA
                     FCLVVVVAGFIAAVAVIANL"
     gene            complement(1493..>2428)
                     /locus_tag="VFB80_08915"
     CDS             complement(1493..>2428)
                     /locus_tag="VFB80_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZN33927.1"
                     /translation="AAPPPPPVPPESSFAWPPGAGPAPAATPAPSTPPEPKNPFSSVN
                     LPAGSAPPPKPANPFGERQAGSPFGGPATGSLNPYASPASAGYQQPTWARTGLPWENE
                     KKTLGCWFRTFGIILGSPSRAFSIMRQTGGLGAPILYNMLGLGMPLALIGMIVVLISL
                     VIMIAGVAGGNGANDAAAAIGIGLGMMGATALFLLVYVVLAATIGNFIAAGFWHLSLM
                     LCGGARQGYETTFRVTGFTYGSLVWLAFIPYVGGFAMMIWLIVLLIIGLSKAHEIPSG
                     KAALAVLLPFGLCCGAYIAFIVLMISGAILSAPAR"
BASE COUNT          451 a          811 c          822 g          344 t
ORIGIN      
        1 cggcgattgg cggtattcgc aggctttttc gcatttcgag cacccgatta attcgccaaa
       61 cagcctcgat acgtggggat ctggcgattt attcgccggc gccggcccca agtgccttac
      121 agcgtcagca gttaggtgcg aaatgccggt ggtgaataat tcgccgccgc gtcgcagtca
      181 ccgtctctcg cgcaacaagt gtgcgcagca ccgagcggcg cgatcgcagc cgccctggcc
      241 gaatttccaa agaacatccc gccgccgaca aagccggcgg tcgcttggaa ttctaatgat
      301 ccgtccacta tctgtcaagt caaccacaac agattgtggt cgccttccgc caaaccacaa
      361 caggtagtgg tcggggcact tctcgggccc ccgaaagagg gcccgccgtt tggggcagtc
      421 tgaatcacag attggcgatc acggccacgg ccgcgatgaa cccggccacg accacgacca
      481 aacagaacgc caccggcagc agcactgcgg cggcggcccg gccggcggtc gtctcgtggg
      541 cctgggccag gccctggatc tggcagatca gcgtcatcac cagggcgatc agcgggccga
      601 agcagggcac gacgctgagc caggcgatcg acccggcggt gaacgcggtg acgcggtagg
      661 tcgtttcgta gccctgatgt gcgccgccga cgagcatcag gcagaggtgc gtgacgcccg
      721 cgctgatcag cagcccgccc gtggcccaga ccaccgtgat caggccgttt agcagcagct
      781 gaaccatgac ctccatgccg gcgacctggc cgcccgctgg attcgcggcc gggccggcga
      841 aggcctcgaa catcattgcc ggcaagctcc acgcggtctg gcccacgaat cccagcaaga
      901 gcccgaagac cgcatacaac atcggcgagc cgatgcctcc gtattgccgc atccgccgga
      961 acgcgctctc cgcgtcgctc aggcacatct gagacgtttc ccaccacacg cccaggcccg
     1021 gcggcttcgt ctcccagggc agccccagct cgcccggctg cgaagggtat gcggaatacg
     1081 ccaccggcgc cggcgaggcg tacgggttgg cgctggccgc ggcctgatcg ccgaaaggat
     1141 tggccggctt gtcggcgaag ggcgcggcgg ccggctgctg ataagcgccc ggctgctgat
     1201 aagccgcagg cggcggagca ttcggcgtca ccgcctgctt cagataggcg aacagatcgt
     1261 cctgcccggg agccgccggc tgctgcgggg cgggaggcgg cgcagcgggt gctaccggct
     1321 gactgatcgc tggctggttg agcggcggga gcgagcgcga cgccaggtcc cccgccgccg
     1381 gcacgaccat caccgtcttg cagcccgggc agcgagcgcc cttccccgcg ctcgaatcgg
     1441 cgacgcggag catttgcccg cactgagtgc agtggaactc gatcgccatg cgtcaccttg
     1501 ccggggccga caggatcgca cccgagatca tcaggacgat gaaagcgatg tacgcgccgc
     1561 agcacaggcc gaacggaagc agaaccgcca gggcggcctt gcccgacgga atctcgtgag
     1621 ccttggaaag gccgatgatg agcagcacga tcagccagat catcatcgcg aagccgccga
     1681 catacggaat gaaggccagc cagaccagcg agccgtaggt gaagccggtg acgcgaaacg
     1741 tcgtctcgta gccctgccgc gctccgccac acagcatcag cgacaagtgc cagaagccgg
     1801 cggcgatgaa gttgccgatc gtggcggcga gcacgacgta aacgagcagg aacaacgccg
     1861 tcgcgcccat catccccagg ccgatgccga tagccgccgc ggcgtcgttc gccccgttcc
     1921 ccccagcgac gccggcgatc atgatgacca gggagatcaa caccacgatc atcccgatca
     1981 aagcgagcgg catacccagg ccgagcatgt tgtagaggat cggcgcgccc agaccgcccg
     2041 tctgcctcat gatcgagaag gcccgcgagg gggagccgag gatgatgccg aaggtgcgga
     2101 accagcagcc gagcgttttc ttttcgttct cccaaggcag cccggtccgc gcccaggttg
     2161 gttgctgata gccggccgac gcgggcgagg catagggatt gaggctgccc gtcgctggac
     2221 cgccgaaggg gctgcccgct tgtcgttcgc caaacggatt ggcgggcttc ggcggcgggg
     2281 cgctgccggc gggcagattc acggacgaga acggattttt gggctcgggc ggggtcgaag
     2341 gcgcaggcgt cgcggccggg gcgggcccag cgccgggcgg ccaggcgaac gagctttccg
     2401 gcggaacagg cgggggcggc ggagcggc
//
LOCUS       DASRJB010000336         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3477907, whole genome shotgun sequence.
ACCESSION   DASRJB010000336 DASRJB010000000
VERSION     DASRJB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477907"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1062
                     /gene="obgE"
                     /locus_tag="VFB80_08920"
     CDS             58..1062
                     /gene="obgE"
                     /locus_tag="VFB80_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZN33928.1"
                     /translation="MFVDRVTIEVQGGRGGNGCVSFRREKYVPHGGPDGGDGGEGGSV
                     IVVAKNGVNNLAALAHHRFWRAESGLPGSGANKQGKSAHDLVIEVPPGTLIVDSHGGF
                     VLKDLTQDGETVTVARGGAAGRGNAHFKTATNRAPRDSTPGGEGERRELVLELKVIAD
                     VGLIGKPNAGKSTLLSRLTRARPQIADYPFTTKHPNLGLVRVDGDRQFVMADIPGLIE
                     GAHAGAGLGHEFLRHIERAGILVHLVEPLPADGSDPLVNYRVIRDELTQYDARLAQRP
                     EILVVSKAELPGAADLREQLAAASGQDVLLISAVTGQGLNTLVERASGLLVAQLQAAP
                     "
     gene            1059..1829
                     /locus_tag="VFB80_08925"
     CDS             1059..1829
                     /locus_tag="VFB80_08925"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZN33929.1"
                     /translation="MSQPRLIAVDIGNSATKIGWFGPALAGLPNPEAVHVFPTGSSPP
                     AELAASLPRETCQWHVSSVHREGTRILQEWVRAHRPADPVRLLTHRDLPIRAAVDFPD
                     KVGLDRLAAAVAVNVIREAGRAALLVGAGSAITVNRISADGVFEGGAILPGFKMQADA
                     LFGGADLLPLALLVPDDAPPQLLGKNTEAAIRSGLFWGAVGAVRELIARLSAGLNPQP
                     KLFVTGGDLRQLAAHLEGARFVPNLVLCGIAVAYKPDA"
     gene            1961..>2191
                     /locus_tag="VFB80_08930"
     CDS             1961..>2191
                     /locus_tag="VFB80_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33930.1"
                     /translation="MAATPDRVLKLCRVLKGLEPREQLSLADYLRDIPRLDSLANVAR
                     GTPVLVRGDVDCKPGPEVGQEDIRLRSMKETLE"
BASE COUNT          334 a          764 c          737 g          356 t
ORIGIN      
        1 tcttcggttt tttggttctt cggttcttcg gttctttggt tcttggtttt tgttcccatg
       61 ttcgtcgatc gcgtaactat cgaggtccaa gggggccgcg gcggcaacgg ctgcgtcagc
      121 ttccggcgcg agaaatacgt cccgcacggc ggccccgacg gcggagacgg cggcgaaggg
      181 ggcagcgtca tcgtcgtcgc caaaaacggc gtgaacaacc tcgccgccct ggcccatcac
      241 cgattttggc gggccgagag cggcctgccc ggcagcggcg cgaacaagca gggcaagagc
      301 gcccacgacc tggtgatcga agtgccgccg ggaacgctga tcgtcgattc gcacggcggt
      361 tttgtcctca aggacctgac gcaggacggc gaaacggtca ccgtggcccg cggcggcgcg
      421 gccggccgcg gcaatgccca cttcaagacg gcgaccaacc gcgccccgcg cgattccacg
      481 cccggcggcg agggcgagcg gcgcgagctg gtcctggagc tgaaggtcat cgccgacgtc
      541 ggcctgatcg gcaagcccaa cgccggcaaa agcaccttgc tgtcgcggct gacgcgggcc
      601 cgcccgcaga tcgccgacta tcccttcacc accaagcacc cgaacctggg cctcgtccgc
      661 gtggacggcg accggcaatt cgtgatggcc gacattcccg ggctgatcga aggggcgcac
      721 gccggcgcgg gtctgggaca cgaattcctg cggcacatcg agcgagccgg catcctggtc
      781 cacttggtcg agcccctgcc agccgacggc agcgatccgc tcgtcaacta ccgcgtcatt
      841 cgcgacgagc tgacccagta cgacgcccgg ctcgcccagc ggccggagat tctcgtggtc
      901 tccaaggccg agctgcccgg cgcggcggac ttgcgcgagc agctcgcggc cgccagtggc
      961 caggacgtgc tgctgatctc ggccgtcacc ggccaggggc tgaacacgct cgtcgagcgg
     1021 gccagcgggc tgctcgtggc ccagttgcag gccgccccat gagccagccc cgcctgatcg
     1081 ccgtcgatat cggcaacagc gcgaccaaga tcggctggtt cggacccgcg ctggcgggct
     1141 tgccgaatcc ggaggccgtg cacgtttttc ccaccggcag ttcgccgccc gcggaacttg
     1201 cggcgagcct gccgcgtgag acctgccagt ggcacgtctc gagcgtccac cgcgagggca
     1261 cgcgaatact gcaggagtgg gtccgcgccc accggccggc cgatcctgtg cgcttgctga
     1321 cccatcgcga tctgccaatc cgcgcggccg tcgatttccc cgacaaggtc ggcctcgacc
     1381 ggctggcggc cgcagtggcg gtgaacgtga tccgcgaggc aggacgggcc gcgctcctcg
     1441 tcggcgcggg ctcggcaatc accgtcaacc gcatcagcgc cgacggcgtc tttgaaggcg
     1501 gcgcgattct gcccggcttc aagatgcagg ccgacgccct gttcggcggc gcggacctgc
     1561 tgccgctggc cctgctcgtg cccgacgacg ctccgccgca gctcctgggc aagaacacgg
     1621 aagccgccat tcgcagcggc ctgttctggg gcgcggtcgg cgcggtacgc gagcttatcg
     1681 ccaggttgtc cgccggcctg aatccacagc cgaagctatt cgtgaccggc ggcgacttgc
     1741 ggcagctcgc cgcgcacctg gaaggcgcgc gcttcgtgcc caacctcgtc ctctgcggca
     1801 tcgcggtcgc atacaaaccc gacgcgtaag cgagggactc tctgggacaa cgtcgctctg
     1861 tggagtccct cgcttacgct tcgggttagt atgctgatcc cctgggaagt ccggccggcg
     1921 attggtacgt tggacgagcc tctcgactcg gagttctccc atggccgcca cgcccgatcg
     1981 cgtcctgaag ctctgtcgcg tgctcaaggg cttagaacct cgcgagcagc tgtcgctggc
     2041 cgattacctg cgcgatattc cgcggctgga ctcgctggcg aatgtcgctc gcggcacgcc
     2101 ggtgctggtc cgcggcgacg tcgattgcaa gccgggcccg gaagttggcc aggaagacat
     2161 ccgcttgcgg tcgatgaagg agacgctcga a
//
LOCUS       DASRJB010000337         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2062792, whole genome shotgun sequence.
ACCESSION   DASRJB010000337 DASRJB010000000
VERSION     DASRJB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062792"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFB80_08935"
     CDS             complement(<1..217)
                     /locus_tag="VFB80_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN33931.1"
                     /translation="MSLTRRSFLSTAAAATGLAAADMTALNVLQSPALASELKSSGKR
                     VILLWLAGGASQLETFDPKPGRPTGGPF"
     gene            complement(270..1895)
                     /locus_tag="VFB80_08940"
     CDS             complement(270..1895)
                     /locus_tag="VFB80_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN33932.1"
                     /translation="MRFRCITLLIAIASFAAHATALGEELLPVDTPIEQAIDHYIEAR
                     LQRAGVSPAPQADDPNLLRRLTLDLVGRIPTAAEARAYVASADSSKRQQLVERLLATP
                     AFVRQQTAEFDALLMDGTKSSIKEYLAKAFRENRTWDRMFREMIVGEPDDAKQQGAIR
                     FVAARAKDQDKLTVDTSVLFFGVNVSCAKCHDHPLVADWKQDHYYGMLSFFSRTFEVG
                     EFFGEREYGKVSFKTVEGESKDAKLMFLTGTVLDEPQAAEPDESAKKEEKKRLEELKK
                     NKEPPPAPSYSRRSALVEAALKDDRSEFFARAIVNHVWNRFFGRGLVNPVDQMHSENA
                     ASHPELLAWLARDVRAGGYDLRRLIRGIVLSQAYSRSSRWDSADKRPPDSLFAVGIVR
                     PLKPWQYGTSLLVAAQSPDSFPADMSAEDFDKRMEGLEGAGRGLCSQFELPAADFQVS
                     VDEALLFSNGQKVAQELLRDGKDRLLGKLADCPDAKSAAETAVWNVFARPPADEEIEA
                     MTTFLSQRSDRMAEARKQLVWALLTSGECRFNY"
     gene            2425..3306
                     /locus_tag="VFB80_08945"
     CDS             2425..3306
                     /locus_tag="VFB80_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN33933.1"
                     /translation="MRRYVFRLAIVTLALLGASAVRGDDQQIAQQIIEKYKAAKSSGS
                     LQGFSIDLQVDEGTVWLSGRVANEQQQAQALDIARRVPGVKQVVNDLEIAVASKNVSA
                     TQRLASSMGKYFRRDDSGSEGRSMIQPASANEGGAIGSGIDPAASGRRQAGNVPYAAA
                     PAAPRYAANPGYAANAGGMPLAMAPARTINYQGAVNGTPGAPIPMHLPGSGVGIAPAR
                     YDHPSMPGYAWPSYAAYPNYAAVTYPKQYSPTAWPYIGPFYPYPQVPLGWRKVTLEWD
                     DGWWFLDFKDRRHPYYH"
     gene            3661..4236
                     /locus_tag="VFB80_08950"
     CDS             3661..4236
                     /locus_tag="VFB80_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33934.1"
                     /translation="MTHRTAMAAIGAIVVATSLASTGCVGLKAGPNIGPLAIPIPISP
                     YFQKKAEDKFWNAERYERAPILGPILPGGPMVALDPPSDDEVVRAWEKADPIQGGLPF
                     LHERQRNNVRIVKEKIADYVDPPRVVPLIGPVQLHHAHYKCTIYYTEVTRVGWPVPHT
                     LVDEDSQEVIYIDHNHFHMVGPVNTGVGANF"
     gene            4333..>4748
                     /locus_tag="VFB80_08955"
     CDS             4333..>4748
                     /locus_tag="VFB80_08955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN33935.1"
                     /translation="MPLTPEQRPSQSARGWIEPELALLLAIVCVIYLTRLAAPNLRGE
                     ETRRGRVAVEMIQTGDWIVPRQQGQLFLSRPPLQNWLIGLCGLVRGEVDWLAIRLPSA
                     LATILLAGLIYSYARGCLSPAGSLVAGLSFATMIQV"
BASE COUNT          847 a         1548 c         1506 g          847 t
ORIGIN      
        1 gaaacggccc gccggtcggg cggccgggct tgggatcaaa ggtctcgagc tggctcgcgc
       61 cgccggccag ccagagcagg attacccgct tgccgctgga cttcagctcg ctggccagtg
      121 ccgggctctg caacacgttg agcgccgtca tgtcggcagc ggccaggccg gtggccgcag
      181 cggcggtcga gagaaaggag cggcgggtga gggacatggg actctccaca aactgttgga
      241 atcgggaggc taacgcctcc cgctcgccac tagtagttga accggcattc tccgctggtg
      301 agcagtgccc acacaagctg cttgcgggct tcggccatcc gatccgaacg ctgcgacagg
      361 aaggtggtca tcgcttcaat ctcttcatca gccggcgggc gggcgaagac attccaaacc
      421 gcagtctcgg cggcggactt cgcatccggg cagtcggcga gcttgccgag gaggcggtcc
      481 ttaccgtcgc gaagcagctc ctgggcgact ttctggccgt tgctgaagag cagagcttca
      541 tcgacgctga cctgaaaatc ggcggcaggc agctcgaact ggctgcacag tccgcggccc
      601 gcgccttcca ggccttccat ccgcttgtca aaatcctcgg ccgacatgtc ggccggaaag
      661 ctgtcgggac tctgcgccgc gacgagcagc gacgtgccgt attgccaggg cttcagcggc
      721 ctgacgatcc caaccgcaaa caggctgtcc ggcggacgct tgtcggccga gtcccagcgg
      781 ctgctgcggg agtaagcctg actgaggacg atgccgcgga tcaagcgccg caggtcgtag
      841 ccgcccgcgc gcacgtcgcg ggccagccac gccagcagct ccgggtggct cgcggcgttc
      901 tccgaatgca tctgatcgac gggattcacc aggccgcggc cgaagaagcg gttccagacg
      961 tggttgacga tcgcccgcgc gaagaactcg cttctgtcgt ccttgagggc cgcttcgacc
     1021 aatgccgacc gccggctgta gctcggcgcg ggcggcggtt ccttattctt cttgagctcc
     1081 tcgaggcgct tcttctcctc tttcttggcg ctctcgtcgg gctcggcggc ctggggttcg
     1141 tccaggaccg tgccggtgag gaacatcagc ttcgcgtcct tcgactcccc ttcgaccgtc
     1201 ttgaagctga ccttgccgta ttcccgctcc ccgaaaaact cgccgacttc gaatgtgcgg
     1261 ctgaagaacg acagcatccc gtagtaatga tcctgcttcc aatcggcgac cagcggatgg
     1321 tcgtggcact tcgcacagct cacattgacg ccgaagaata gtacgctggt atcgacggtc
     1381 agcttgtcct ggtccttggc gcgggcagcc acaaagcgga tcgccccttg ctgcttggcg
     1441 tcgtccggct cgccgacgat catctcgcgg aacatccggt cccaggtgcg gttctcgcga
     1501 aaggccttgg ccaggtattc cttaatgctg ctcttggtgc cgtccatcag cagggcgtcg
     1561 aactctgccg tttgctggcg gacgaatgcc ggcgtcgcga gcaggcgctc gacgagctgc
     1621 tgccgcttgc tgctgtcggc tgaggcgaca taggcccgcg cctccgccgc cgtgggaatc
     1681 cggccgacga gatccagcgt caatcggcgg agcaggttcg ggtcgtcggc ttgcggcgcg
     1741 ggcgagactc cggctcgctg caagcgagcc tcgatatagt ggtcgatcgc ctgctcgatc
     1801 ggcgtatcga caggcagcag ctcctcgccc agcgctgtgg catgcgcggc gaagctagca
     1861 attgcaatga gcagtgtgat acagcgaaat cgcatgcagc aagtcctcag gcatgcaatt
     1921 gggtgggagg ttctggcggg acttgtagga taacgggtcg ccgggcgtga tgccagcaga
     1981 atacgacgga gagacagaga gacggagggg gctcgctccg tccctccgtc cctccgtcgc
     2041 tccgtcattc ccaccgcagc cgttaaacct ttccacggtt gagcttggag caaggaaaag
     2101 tcgcagccgt tacgaccgcg aagcacggca aaatcgaaaa aaaccttccg tccggcacag
     2161 ccggaatccg acaagtaata tcgggcagac gtgtttcctg ccgagcttgc gccggggggc
     2221 caggcgagcg gcggccgaat gctgggccgc ctccttcccc cttcgcctgc acgctggacc
     2281 gccgccccgc gccagatagc ggcgcgagat aggcccgcaa ttcaccaggc gccttcgccc
     2341 tcacgcggaa gagaccccat ccgcgtaggc gaacaggcgc caagccgacc cagcgagacg
     2401 gcagtcaacc tcaggagggg gaaaatgcga cgttacgttt tccggctggc gattgtcacc
     2461 ctggccctgc tcggagcgag cgcggtccgc ggcgacgacc agcaaatcgc gcagcagatc
     2521 atcgagaagt acaaggccgc caagtcgtcc gggtcgctgc aaggattcag catcgacctg
     2581 caagtcgatg aaggcacggt ctggctttcc ggccgcgtgg ccaacgagca gcagcaggcc
     2641 caggcgctgg acattgcccg ccgggttccc ggcgtgaagc aggtggtcaa cgatctggag
     2701 atcgccgtgg cgtcgaagaa cgtcagcgcg acccagcggc tggccagctc gatgggcaag
     2761 tacttccgcc gcgacgactc gggcagcgaa ggccgctcga tgatccagcc ggcctcggcc
     2821 aacgagggcg gcgcgatcgg ctcgggcatc gatccggccg cttcgggccg ccgtcaggcg
     2881 gggaatgtgc cgtacgcagc cgctcccgcg gccccgcgct acgcagccaa ccccggctat
     2941 gccgccaatg ctggcggcat gccgttggcg atggcgcctg ctcgcacgat caactatcag
     3001 ggtgcggtga acggcacgcc aggcgctccg attccgatgc acctgcccgg cagcggcgtc
     3061 ggcatcgccc cggcccgcta cgatcacccg tcgatgcccg gctatgcctg gccgtcctac
     3121 gccgcttacc cgaactacgc ggccgtaact tatcccaagc agtattcgcc gacggcctgg
     3181 ccgtacatcg gcccgttcta tccctatccg caagtgccgc tgggctggcg aaaggtgacg
     3241 ctggaatggg acgacggctg gtggttcctg gacttcaagg atcgccgtca tccgtattac
     3301 cactagtcgc aagctgagtt tgaatatcac gaccctcgcg gagctgctgg cttcgcgggg
     3361 gtttttgttt tttggtcagt tgtcagtggt ccgtggtcag ttggggcaag ccgttccaac
     3421 tgacaacgga ccactgacta ctgacaaaac cgggacaacg ccgctagcag atatttggaa
     3481 tgctgccctc aaagttcacc tgaatttcct gcgcaacccg aaaaatccgc aagttcgata
     3541 cagcttgaca cgataaatag gtctgagaat cgacgggcat cgcgcccggc ggttgatagc
     3601 atcgtccgcc agcgaattga atccagccgg ggcggccggc cacgacattg aggttcagtc
     3661 atgactcaca ggacggcaat ggcggccatc ggggcgatcg tggtggcgac gagcctcgcc
     3721 agcaccggct gcgtgggcct gaaggctggt cccaacattg gaccgctggc gattccgatt
     3781 ccgatcagcc cgtacttcca gaagaaggcg gaggacaagt tctggaacgc cgagcgttac
     3841 gaacgggcac cgatcctagg tccgatcctg ccgggcgggc cgatggtggc cctcgatccg
     3901 ccgagcgacg atgaagtcgt gcgggcctgg gagaaggccg acccgatcca gggcggcttg
     3961 cccttccttc acgaacgcca gcgaaacaac gtccggatcg tgaaagagaa aatcgccgac
     4021 tacgtcgatc cgccgcgggt cgtgccgcta atcggacccg tccagctgca ccacgcccac
     4081 tacaagtgca cgatctacta caccgaggtc acccgcgtcg gctggccggt gccgcacacg
     4141 ctcgtcgatg aagattcgca ggaagtgatc tacatcgacc acaaccactt ccacatggtc
     4201 ggtccggtca acacgggcgt cggggccaac ttctagttcg tcttgactcg ccccgcaatt
     4261 ctcgcaaata tccgccccgc tagctgccgc tagcggggtt ttttcgtcgc cggatccttt
     4321 gcgagttgca ccatgcctct tacgccagag cagcgcccgt cgcaatctgc ccgcggatgg
     4381 atcgaaccgg agctggcact gctcctggcg atcgtgtgcg tgatttacct cacccgcctg
     4441 gccgcgccaa acctgcgcgg cgaggaaacg cgccgcggac gcgtcgccgt ggagatgatt
     4501 cagaccggcg actggatcgt gccgcgtcag cagggacagc tcttcctcag ccgcccaccg
     4561 ctgcaaaact ggctcatcgg tttgtgcggc ctggtgcgtg gcgaagtcga ctggctggcg
     4621 atccgcttgc cgtccgcttt ggccacgata cttttggccg gtctgatcta tagctacgcc
     4681 cgcggctgcc tgtcgccggc tgggtcgctc gtcgccgggc tgtcgttcgc gacgatgatc
     4741 caggtcat
//
LOCUS       DASRJB010000338         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3197495, whole genome shotgun sequence.
ACCESSION   DASRJB010000338 DASRJB010000000
VERSION     DASRJB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197495"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..899)
                     /locus_tag="VFB80_08960"
     CDS             complement(9..899)
                     /locus_tag="VFB80_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN33936.1"
                     /translation="MLSRRELLQSSLLAGSAAAISSVASAIEPIARNGKSKFKFSLAA
                     YSYRGLLAGDKATLTLKDFVDDCAKFGLEGTELTSYYFPKDTTPDYLRALRRQAFRLG
                     LDISGTAVGNEFGHPAGEKRAAEIAKVKQWVDNSEILGAPVIRIFAGHQQKDQTAAQA
                     HSLMVSAMEECCDYAGKHGVHLALENHGGPTGSADGILEFVRDVSSPWFGINLDTGNF
                     QSTDPYADMAKLAPYAINAQIKVDVSIGGKRTPADFKRVAKIMRDANYRGYIVLEYEE
                     AGDVRVECPRYLDQLREAFA"
     gene            1234..2061
                     /locus_tag="VFB80_08965"
     CDS             1234..2061
                     /locus_tag="VFB80_08965"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZN33937.1"
                     /translation="MQGLYEKVEAAAAFIRQAWKGTPHAGIILGTGLGSLVKHIQEEA
                     SLEYGEIPHFPRSTAISHRGRLVCGRLAGLSVLAMEGRFHMYEGYPLDQITLPVRVMK
                     ALGAKLLVVSNACGGMNPYYRAGDIMLIDDHINLMGANPLIGVNDDRLGPRFPDMSQP
                     YSHKLIDRALEIARRENIVAHKGVFVAVSGPNLETRAEYRFLRLIGADVVGMSTVPEV
                     IVAVHAGLSVVGFSIVTDMCLPDALEAADVAKIIATANAAEPNLTKLVLGVLAGEKP"
     gene            2094..2894
                     /locus_tag="VFB80_08970"
     CDS             2094..2894
                     /locus_tag="VFB80_08970"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZN33938.1"
                     /translation="MPDSPAIDAAVAAIHRRWDGPAKTAVILGTGLGDLADEITAEAI
                     IPYRDIPGFPRSTALAHKGRLVCGRQDGAPVVMMQGRCHLYEGYSLAEITLPARVLVA
                     LGVRTLIVTNAAGGVNPNYCVGDVMLIDDHINLMWLGALDPEPVPRPFKRQRLYDAEL
                     ARRAEQIARRGDFALRRGVYLAVTGPSYETRAEYRAFRRLGADCVGMSTVPEVLVAAA
                     AGLSVFGLSTVTNVARPDAPQTVSAEEVVEVAATALPKVRAIIREVVR"
BASE COUNT          548 a         1104 c         1053 g          607 t
ORIGIN      
        1 tagccctctt aagcaaacgc ctcgcgcagc tgatcgagat acctcgggca ctcgacgcgc
       61 acgtcccccg cttcctcgta ttccaggacg atgtagccgc ggtagttcgc gtcccgcatg
      121 attttggcga cgcgcttgaa gtcggccggc gtgcgcttgc cgccgatgct gacatcgacc
      181 ttgatctggg cgttgatcgc gtagggagcc agcttggcca tgtcggcata gggatcggtg
      241 ctctggaagt tgccggtgtc gaggttgatg ccgaaccagg ggctgctcac gtcgcggacg
      301 aactccagga tgccgtcggc cgagccggtc gggccgccgt ggttttccag ggccaggtgc
      361 acgccgtgct tgccggcgta gtcgcagcat tcctccatcg ccgagaccat cagcgaatgg
      421 gcctgggccg cggtctggtc cttctgctgg tggccggcaa agatgcggat caccggagcg
      481 ccgagaatct cgctgttatc gacccattgc ttgaccttgg cgatttccgc ggcccgcttc
      541 tcgcccgcgg gatgcccgaa ctcgttgccg acggccgtgc cagaaatatc gagccccagg
      601 cggaacgcct gccgccgcag cgcccgcaga tagtcgggcg tcgtgtcctt cgggaagtaa
      661 tacgacgtca gctcggtgcc ttccaggccg aacttcgcgc agtcgtcgac gaagtccttg
      721 agcgtcagcg tcgccttgtc gccggcgagc agcccgcggt agctgtacgc cgccaggctg
      781 aacttgaact ttgacttgcc gttgcgggca atcggctcga tggcgctggc gacgctagaa
      841 atcgcggcgg ccgaaccggc gagcaggctg gactggagca gttcgcggcg ggagagcatg
      901 gcgggcgcct ttctatagtg gggcgagtgc taatgaggtg ataatacccg atgctccagg
      961 catagtgaga gcggcaggcg tacttgtggc ggttagctgt cctcgatagt tttgcgtgcg
     1021 tcgccgtcgc agatgttcag ccgcggagcg gcggcagtca atagccggca ccgtcaggtg
     1081 ccggactgcg tgatacgcga tgacatcgag gcccggaggg ccgacacgaa tccgggtgtc
     1141 ggccctccgg gcctcacgcg agaatcgagc gcgatttccc ccgacctgac ggtcggggct
     1201 acttactttc ggccctccgg gcctgggagc tgtatgcaag gcctctacga aaaagtcgaa
     1261 gccgccgccg cgttcatccg ccaggcttgg aaaggtacgc cgcacgccgg catcatcctc
     1321 ggcaccggcc tgggcagcct ggtcaaacac atccaggaag aagcctcgct cgaatacggc
     1381 gagattccgc acttcccgcg gagcacggcg atcagccacc gcggccggct ggtctgcggc
     1441 cggctggcgg ggctgagcgt gctcgcgatg gagggccgct tccacatgta cgagggctac
     1501 ccgctcgacc agatcacgct gcccgtgcgg gtgatgaagg ccctgggggc gaagctgctc
     1561 gtcgtcagca acgcctgcgg cggcatgaat ccgtactacc gggccggcga catcatgctg
     1621 atcgacgacc acatcaacct gatgggcgcc aacccgctga tcggcgtcaa cgacgaccgc
     1681 ctcggtccgc ggtttcccga catgtcgcag ccgtactcgc ataagttgat cgacagggcc
     1741 ctggaaattg cccgccgcga gaacatcgtg gcccacaagg gggtgttcgt cgccgtctcc
     1801 ggccccaatt tggaaacccg ggccgagtat cgcttcctcc gcctgattgg ggccgacgtc
     1861 gtcggcatgt ccaccgtgcc cgaggtgatc gtcgccgttc acgccgggct ctcggtcgtc
     1921 ggcttttcga tcgtgaccga catgtgcctg cccgacgcgt tggaagccgc cgacgtggcc
     1981 aagatcatcg ccacggccaa cgccgccgag ccaaacctga ccaagctcgt cctcggcgtc
     2041 ctcgccggcg agaagccgta gtcaaagccg cgaccatcgg tcgcgctctg ccgatgcccg
     2101 actctccagc catcgacgcc gcggtcgccg ccatccaccg ccgctgggat ggcccggcca
     2161 aaaccgccgt gatcctcggc acgggtctgg gcgacctcgc cgacgagatc accgccgaag
     2221 ccatcatccc ctaccgcgat attcccggct ttccccgctc gaccgcgctg gcccacaaag
     2281 gccggctggt ctgcggccgg caggatggcg cgccggtcgt gatgatgcag ggccgctgcc
     2341 atctctacga aggctacagc ctggccgaga ttacgcttcc cgcgcgggtc ctcgtcgccc
     2401 tgggcgtgcg gacgctgatc gtcaccaacg ctgccggcgg cgtgaatccc aactactgcg
     2461 tcggagacgt gatgctgatc gacgaccata tcaacttgat gtggctggga gcattggacc
     2521 cggagccagt gcctcggcct ttcaagcgtc agcggctgta cgacgcggag ctcgcccgcc
     2581 gcgccgagca gatcgcccgc cggggcgact tcgcgcttcg ccgcggcgtc tatctggcgg
     2641 tgaccggccc gagctacgag acccgggccg agtaccgcgc cttccgccgc ctgggtgctg
     2701 actgcgtcgg catgtccacc gttcccgaag tcctcgtggc cgcggccgcc ggcctttcgg
     2761 tgttcggcct ttcgaccgtc accaacgtcg cccgccccga tgctccccaa acggtttccg
     2821 ccgaggaagt cgtggaagtg gccgccaccg ccctgccgaa agtgcgggcg atcatccggg
     2881 aggtcgttcg ttaatcgacg accggtggct cggtcccgcc ccatgcgcgc atactcgaaa
     2941 ttagggctcg gggcacaaat ctcgccgcaa cctattgccc cacaatacct tgtgtcgatt
     3001 cggcgggcgc atcgaatatg cgcattatgc gcgccgcgag cgttttccag tttgcgcagg
     3061 gcaagctgcg aacaacctcc tttgcttcac gtgtcaccaa catcagccac atcgaatcag
     3121 gggtaaggat aattcccgat gtggtcctct cctgccaaga cccagattcg tccggccagc
     3181 gtcgcaagac gttatttggc ttgagtttcg gtccgcaggc gagctcgaat ttgacatgac
     3241 gatttgaagc atttgagggg cgagtcggga attatcctta ccctcgttgg cggcacccgc
     3301 agattggact ta
//
LOCUS       DASRJB010000339         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3197696, whole genome shotgun sequence.
ACCESSION   DASRJB010000339 DASRJB010000000
VERSION     DASRJB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197696"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1521)
                     /locus_tag="VFB80_08975"
     CDS             complement(127..1521)
                     /locus_tag="VFB80_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN33939.1"
                     /translation="MYDTLIIGAGFSGLAAGIRLAYYEQRVCILERHYTIGGLNSFYR
                     LRGRDYDVGLHAVTNYAPKGDKKGPLAKLLRQLRLAWDDFGLAPQLGSAIVFPGVRLD
                     FNNDIRLLESEIARAFPRQKENFQRLLGQILDYDDLSQDVFDLSARQILSRTLSDPLL
                     VEMLLCPAMWYGNSREQDMDWGQFCILFRSIFLEGMGRPYAGIRLILKNLVRKFRALG
                     GELKLRSGVRRIVVEGGRATAVVLDSGEELSCRRILSSAGAVETMRLCDKFGEDSRAQ
                     PGRLSFVESLSILDRPPQSLGCGQTVVFFNDSEKFHWQRPEGELCDARTGVICSPNNF
                     AYSDGEGLLADNLLRITALANFDGWQGMSEDEYRLAKLRWYDRLAASAVRFIPDFRSR
                     VIDTDMFTPTTIRRFTGHENGAIYGATHKQLDGTTSVRDLFLCGTDQGYLGIVGALIS
                     GVAMANRHCLLPAP"
     gene            complement(1644..1904)
                     /locus_tag="VFB80_08980"
     CDS             complement(1644..1904)
                     /locus_tag="VFB80_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN33940.1"
                     /translation="MTTAEISHEVIDILSDIAPDEDLSGLVADKPFREQLELDSMDFL
                     DIVMELRKRHRIQIPEEDYKHLASLASTVAYLEPRMRELVKK"
     gene            complement(2004..3269)
                     /locus_tag="VFB80_08985"
     CDS             complement(2004..3269)
                     /locus_tag="VFB80_08985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN33941.1"
                     /translation="MSVHAPDKLPASQRIVITGVGLTAPSGNNLPEFRAALLAGRSGV
                     SKYEIRYVGETLAGICSFDSLRYQSKKDLRRGTRAGSVGIYSASEAVRDAGLDWAQID
                     KSRVGVYLGVTEHGNVETENEIYALKGYDYDTSFWSHHHNPRTVANNPAGEVALNLGV
                     TGPHYTIGAACAAGNAGLIQGAQMLRLGDCDLALAGGVSESIHTFGIFASFKSQGALA
                     AHDDPAKASRPFDAARNGIVVAEGGCVFVLERLDDALRRGAKIYGELAGYAINTDATD
                     FVLPNPERQAECVALAIRRAGLRPHEIDIVSTHATGTGQGDAQECQALRRVFAESTKT
                     YFNNTKSFIGHAMGAAGALELAGNLPAFDDGVCHATINLTDLDPECALTTLVINEPRR
                     IGDVKYILNNSFGMLGINSAVIIKKFTPL"
BASE COUNT          610 a         1081 c         1149 g          638 t
ORIGIN      
        1 cgcgacgcgc ataatgcgca tatcgactgg gccgcgcgca tgaccgcaag ttcttgtggt
       61 gacacgagtt aggcgcagaa tcgccgtccg gaaattttct cggagatgcg cgcatcggcc
      121 gaccggctat ggcgccggca gcaggcaatg ccggttggcc atggcaacgc cgctaatgag
      181 ggctccgacg atgccgagat agccctggtc ggtgccgcaa aggaagagat cgcggacgct
      241 ggtggtgccg tcgagttgct tgtgcgttgc cccgtagatc gcgccgttct cgtggccggt
      301 aaatcggcgg atcgtcgtcg gcgtgaacat gtcggtgtcg atcacgcggc tgcgaaaatc
      361 ggggatgaaa cgcacggcgg acgcggccag cctgtcgtac cagcggagtt ttgccaggcg
      421 atattcgtcc tcggacatgc cctgccagcc gtcgaaattg gccagcgccg tgattcgcag
      481 caggttgtcg gccagcagtc cttcgccgtc gctgtaggca aagttgttcg gcgagcagat
      541 gacgccagtc cgggcgtcgc acagttcgcc ctcgggccgc tgccagtgga acttctccga
      601 atcgttgaag aagacgacgg tttggccgca gcccagcgac tgcggcggcc ggtcgaggat
      661 cgagagcgac tcgacgaacg acagccggcc gggctgcgcc cgagagtctt cgccaaactt
      721 gtcgcacagc cgcatcgtct cgaccgcgcc agccgacgag agaattcgcc ggcaggaaag
      781 ctcctcgccg ctgtcgagca cgacggctgt cgcccggccg ccctcaacga caatccgccg
      841 gacgcccgag cggagcttca gctcgcctcc cagcgcgcgg aacttgcgga cgaggttctt
      901 gaggatcaat cggatgccgg cgtagggccg gcccatgccc tccagaaaaa tcgagcggaa
      961 caagatgcag aactgtcccc aatccatgtc ctgctcgcgg gagttgccgt accacatcgc
     1021 ggggcagagc agcatctcga ccagcagcgg atcgctcagc gtgcggctga gaatctggcg
     1081 ggcggagaga tcgaacacgt cctggctcaa gtcgtcgtag tcgagaatct ggccgagcag
     1141 ccgctggaag ttctccttct gccgcggaaa ggcgcgggcg atctcgcttt ccagcaggcg
     1201 gatgtcgttg ttgaaatcga gccgcacgcc cggaaagacg atcgccgagc cgagctgcgg
     1261 ggcgaggccg aaatcgtccc aggccagccg cagttggcgg agcagtttgg ccagcggccc
     1321 cttcttatcg cccttgggag cgtagttggt gacggcgtgc aggccgacgt cgtaatcgcg
     1381 gccgcggagc cggtagaacg aattcagtcc gccgatcgtg taatgccgct ccaggatgca
     1441 gacccgctgc tcgtaatacg ccagccgaat ccccgcggcc aggccggaga aaccggcgcc
     1501 gatgatcagc gtgtcgtaca tggtagagga acgagggaga gacagaggga cagagtgaag
     1561 gagagacgga gagactgcgt tttccgcttt ctccgtctct ctgtcactct ttctctcctt
     1621 ctctccgtcg ttattgcgga cagttacttc ttcaccaatt ccctcatccg cggctccagg
     1681 tacgccacgg tgctggccag gctggcgagg tgcttgtagt cctcttctgg aatctggatg
     1741 cggtgccgct tgcgcagctc catcacgatg tcgaggaagt ccatgctgtc cagctcgagc
     1801 tgctcgcgga acggcttgtc cgccaccagc ccggagaggt cctcgtcggg ggcgatgtcg
     1861 gagaggatat cgatcacttc gtggctgatt tcggcggtgg tcactgtgtg ctccaaaagg
     1921 acgcgctgta gggaacgccc tctgtggcgt tccgttcggc gagcgacatt cacctgcgga
     1981 gggaatgcca cggagggcat tccctacaga ggcgtaaact tcttgataat gactgcggag
     2041 ttgattccca gcatcccaaa cgagttgttg agaatgtact taacgtcgcc gatccgccgc
     2101 ggctcgttga tcacgagcgt cgtcagggcg cattcggggt cgaggtcggt gaggttgatc
     2161 gtggcgtggc agacgccgtc gtcaaaggcc ggcagattgc ccgccaattc gagggctccg
     2221 gctgctccca tggcgtggcc gatgaagctc ttggtgttgt tgaaataggt ctttgtgctt
     2281 tcggcaaaga cacgccgcag ggcctggcac tcctgagcgt ccccctgacc ggtgccggtg
     2341 gcgtgcgtgc tgacgatatc gatttcgtgc ggcctcaggc cggcgcggcg gatggccagc
     2401 gcgacgcact cggcctgccg ctcgggattg ggcaggacga agtccgtggc gtccgtgttg
     2461 atcgcgtagc cggccagttc gccgtaaatc ttggccccgc ggcgaagggc gtcgtccaat
     2521 cgctccagca cgaagacgca gccgccttcg gccacgacga tgccgttgcg ggccgcgtcg
     2581 aacggccgcg aggctttcgc cgggtcgtcg tgcgcggcga gcgcgccctg gctcttgaag
     2641 ctggcgaaaa tgccgaaggt atggatgctc tcggagacgc ccccggccag ggccaggtcg
     2701 cagtcgccca gccgcagcat ctgagcgccc tgaatcaatc cggcattgcc cgcggcgcag
     2761 gccgcgccga tcgtgtagtg cggaccggtg acgcctaaat tcagggccac ctcgccggcg
     2821 ggattgttgg ccacggttcg cgggttgtgg tggtgcgacc agaagctcgt gtcgtagtcg
     2881 tagcccttga gggcataaat ctcgttctcc gtctcgacgt taccgtgctc ggtgacgccc
     2941 agataaacgc cgacgcggct cttgtcgatc tgcgcccagt ccaggcccgc gtcccgcacc
     3001 gcttcgctcg ccgaatagat gccgacgctg ccggcccgcg tgccgcggcg gaggtctttc
     3061 ttcgactgat accgcaagga gtcgaaactg cagatgcctg ccagcgtttc gccgacgtag
     3121 cgaatctcgt atttgctgac gccgctgcgc ccggccaaca gcgccgcgcg aaactccggc
     3181 aggttgttgc cgctgggcgc agtcaacccc acgccggtga tgacgatccg ctgcgaggcg
     3241 ggcaacttat ccggagcatg cacggacatg acgcttcctg ggcgagcgcg gcaactggcc
     3301 gctaccagga cgtaccgaac gcaaaccacc gaagttaacg gcccaccgca cgactggcaa
     3361 gcggcgggat cggagcgaag ttccggtcca gattcgcttc gtaagtgcta gccgcatcgg
     3421 ctcttagcgc ggcagccgcc gtgacgcgtt gcaagccgtg cggtcggtgc gttcatct
//
LOCUS       DASRJB010000340        22693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4046162, whole genome shotgun sequence.
ACCESSION   DASRJB010000340 DASRJB010000000
VERSION     DASRJB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 22693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22693
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4046162"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..3575
                     /locus_tag="VFB80_08990"
     CDS             297..3575
                     /locus_tag="VFB80_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN33942.1"
                     /translation="MPRNLEPRAGRRERAGGQRPLDRAASARRRLQLEPLESRSLLAA
                     LPFGAMPDDTGEYMLGDVHVTVVLMESNNTIDNGTITYTDTDPNTGGNQTVQYNYPAE
                     NWTTQAIGAVKAKVQAGLDWWEDTLDALPNVRDGLLNFTVDWTYADTPVATGYEPIAR
                     VSNDFTLWVYDFLNKVGFTQTGNFSTDIRAYNNSRREATGSDWAFTIFVVNNAADTDK
                     FFTQILLDSGGSFGSFGQAFSFAGGRFMIVPASRPTGTFAHETGHMFWAFDEYDGSSA
                     SYQSRRGYYNTANSNHVNNPTPGFVHANSIMSNGASLDAAFAGHTSSTSSLETIGWKD
                     SDNDGIFDVLDVPFKLSGIGRYTAATGLFRFTGSASVATLPNQNTSGLQNDITINQLR
                     VVEMAIDNGPWTTVQTLPPRTYTTALDVSFAVPAGQHTVKLRVSDTRTGVTSPEFIGD
                     TGAPDAIGSPGIGGFVFRDDDGDGVWDANEPPLVDYGLDVVDQDDTPIDLRRNLEPSQ
                     FPESTVLNAIHPEATLTAIGGDVVAATEIQRLTITGSTGSSVTPAFGGVNGLPLSIQF
                     GLAPTAADVLASLNAIPALAGKVAVAGANGGPFTVTFSGQLSQVNVPPITQGAASGGA
                     TLAVESVVEGAPAQVIARTSGLYSSLAGKVFTTRSIAAGQLVEKWTSSSRQLKISFQN
                     PVGVVSIRAFSAPLVAGQPSFARLEAYNSQGVLLERFTSGAISGNTFALLSINRSASD
                     IAYVIARGHVGTEVVLDTLQWGATTSATSNPLGAYSLAFLPPGTYRVKADPPSNHTVT
                     TPVGGVATITVAAGQAQNVNFGIRIQASLWHNVAKPLNVDNDPLLAVAPLDALLVINW
                     LNAHPNQPELPSQATPSSDGYIDVTNDGLCTPLDALLVINELNSGGGGGGGGGGGGGG
                     GEGWSGGGGGTGGGGGGEGEMPGSHAPGSDSGDGEGEIAPSAPLSAAEYYARNPIHFL
                     QIAGADVPCGCEECLAQRVAQPIGDSNAAPAKATLTVFDTLESAKAEPSSSRGSAVPE
                     ADPTLQAKVQQTLLAVKKALLPLAARKQKLEQSIDSIAADVSQAQIDVPARLMQRRGR
                     TRNS"
     gene            3730..5151
                     /locus_tag="VFB80_08995"
     CDS             3730..5151
                     /locus_tag="VFB80_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33943.1"
                     /translation="MPDCRFASVAVLLLAALAGCAAAPPAPAPAAPALPTSASVGGAN
                     IVAIAPPGQQCCPKQTLPQFLGIVGACNEIKTLCNNIRNRLGAIWPGLEAKPPVLSLT
                     DPANLQSDNPAVQSAAEVKAAEDGAAQKAKAIAYLASIGCAGCYPGIEEALIAALDDC
                     TELIRFETLKALRQQSMGPCKNCCRSACCSPKMREKMEKLAYELDAQGCYVEPSERVR
                     RMARLALCNCQCVPLKPGQTPAGLPPEGPSPAEAAPPPPPAGATASVLRALPATEQPL
                     GSGVITAQPASRNTAQPAARNIAQPAGGAPARPVSYEEALLSYQPGGRQAEPAAALTA
                     WEIWSARPQDFASRPDAVAALSMARMQLMTSQAGPLPPQLQMQTHGWTDPATIRSPAI
                     ARAVATTPVSGVSGVLEDHLGWHLVRPIARRPAPRPTPAAGNLAQHAATQLPSTATQL
                     QQPAAAPVPPALTTVRLPPCDCH"
     gene            5227..10152
                     /locus_tag="VFB80_09000"
     CDS             5227..10152
                     /locus_tag="VFB80_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33944.1"
                     /translation="MAQSTTSSTAPSGNGPNGRKGWRGAAPAEAPRHRWQVGGGGNGG
                     SVLASRPFRKARVAAWFIAAAALLLCLAYYLLYAPAQTPLFAVVGTSHRRPIPPNAWA
                     AEDLLALRDLEGKSLAVTNLSEDWRSPDHGLKRLGDELSQFVRRHSRTRSIVLYVSLP
                     GAVDAEGRPCLLTPDAAPHDSTTWLPLVKVLGQIQAAEVPAGVHKLLVLDCTREVADW
                     NLGQAYSGFVESLPAALDEAKIPNLAILCSAGPGQSAAVSPALRGSVFGHYLRLGLAG
                     AADLRGFGGNGDHRVTLAELEQYLRGHVHGWARHHLGRSQIPQVIPQEANFTVTWSLR
                     RGDLEELRARSGRIEAADPIVPAGEIAQLWNKLDTLRQARPWRFDPAGWRMLSFDLLR
                     LEQLTAAGKAYEAEAGQLRGELATRLNQAEERRQAAARSGSLPAFAAILDGAAGPPLK
                     MHSLSMAALWGTADADALRALGAEMARVAEEPSPANIAAALGAGEAARLPAALDETNL
                     LAIWQRLAVPAAWRDPAPLALAWQVHSLAEQHAAPLSLDGLPGDERAHAWVRGPLAQL
                     DADRRQLEDAVLVGPALDNALAPGQDTRQALESFTLSERVTAACALRDDLWAELPHLA
                     AWLCRPQELADDEAAADRLIEKTLRPLLAEAIHFEEHLERTGQGRAGELDWTGDALVA
                     SIDQKFDELRSNFDGRARALATAVLATTTERAAAAREIDAALATPILRWDQRQRLREK
                     RSELHVKLAADYDAIQDGAAQPAAAAAPALPHLVRMSRWQPHPLAALLDHATVLDSAE
                     RRAAAETAEGDRLRAALELNEAARQRLLALSRGPVKSLVTGQAQNVPVDWKSLCQAES
                     RLRSAAALWFEPPGVDPVRELRRADLAQLFLWRAQRALDDYYGPARPGGEAFFAASAA
                     DYLAAARSFGPLTEAADAQAAGLDRLLSQRRIAAREALGVTATDILLVEQLATVDSSL
                     DVRTRTPEAAANLPQATAAVFLSDQQGRIGPPGWPLAVPQPDLKADQPLLAHNMTLDT
                     AGLKSRGPLVQAVASVRGNDFAAPLLLRAPGGLRVEYRPILYGPPQVTVLGFDRKKAS
                     VVFILDCSHSMSAPVAVERPDEAVRTESTRMEVAKGSLRSLLAELAARGDLRVGVRLF
                     GHRVGWSTTKADELLRQTGYAEPIPAELRPYADVQEILDLGRFDTTIAAKVYDQLQTV
                     KPWGESPIFLALQQAIADFGADDNSARSVVLITDGQNSQFNPPREFAPALADVLAAAQ
                     RAGVAIHVVGFDIPTNEATAARRDFEQIAAASGGSFVPAKDSTALVETLQTLLRPGQF
                     RVDDAGGNVLAQAEIGRSITLRNHRGREDYGVAFENMREPVELFGGEAVELTVRRGSP
                     RLEVVPYLKGNPQPQNMIVQEDGAATPLVAWLHRSVRTEQGIRFPISIQHADLHFVPR
                     PVEMWVEVAPLGLPPQEDPGPYIFYDAPLEAGTTVPLATFLAQNWPAGAPRAEIRVWA
                     KSTATEPTQSRQLTEVADRLPTEGAGFEVPGLAGVTYQVRTAGGNGQLSVGVIERHDE
                     RSPGVGSLKVTLAPPASRALHQFDAANRLVLHTFTYEQPAEDLRGRLSIQFTSRGDAL
                     ARSWRTAQPAIVDVSDRLDLLEVTPPVGANR"
     gene            10240..10482
                     /locus_tag="VFB80_09005"
     CDS             10240..10482
                     /locus_tag="VFB80_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33945.1"
                     /translation="MADKLRQVNVLLQALARVPGLGFLAQAEYRLQDAVDAVDDVNDN
                     IEDNQRYMADVGKAVTDITSSEDEEQPSEASESSDS"
     gene            10555..12528
                     /gene="tssI"
                     /locus_tag="VFB80_09010"
     CDS             10555..12528
                     /gene="tssI"
                     /locus_tag="VFB80_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HZN33946.1"
                     /translation="MAQAVTVTSPFGDGKLLFQSLTGREELGRMFHYKLEALSENGAL
                     DLNKILGQNITIKAEFPDDRVRYFNADVIQFRQITKSVDKYFCYEAQLRPRLWFLTRT
                     ADCKIFQKKSVPDIVKAILDELGITDVKKSLSKTYNPREYCVQYNETDFDFISRLMEE
                     EGIYYYFEHASGKHTLVLADSLSAHESITKKDPLPFRLRKAGYQEDHIYDWNMQQQVE
                     SGTWAHLDFDFVKPRADLKSQSVITRQHTHASMELFAYPGGYLEKADGSAMARTRIEE
                     VQVPWEMISGNTTAVTMATGKLFKMSGYAREDQNREHLVIASNIEMKMEIDTAAAAGS
                     DVLDSKAGVYFDCKFEAIGSQRPFRTACRTPKPVVKGPQTALVVGKSGEEIWTDQYGR
                     VKVQFHWDRLGEKNENSSCWLRVAQIWAGKQWGAMHLPRIGQEVIVDFLEGDPDQPIV
                     TGRVYNSEQKVPYDLPANQTQSGLKSRSTKEGDAETFNELRFEDKKDEEQIYFHAQKN
                     FDRVVENNDTLKVGFEKKDKGDQTIDIYNNRTVTLEKGNDKLQIKEGNRTELIDKGND
                     ELTIAQGNKTITISQGNLTIKIDAGKASIEAAQEILLKVGESTITIKPSAIELASVNV
                     KTTATGNFEASGAQSKVAGSGMLDLDGGTITMN"
     gene            12538..13503
                     /locus_tag="VFB80_09015"
     CDS             12538..13503
                     /locus_tag="VFB80_09015"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN33947.1"
                     /translation="MQLTLQITAGPHAGRKVLLRSGQVARIGRTEWADFSFPQDAELA
                     DVHFAVQCQLHGAKVRKLAADKPLAVNKQQVTEAELKAGDTIQAGQSAFVVVFDGAAA
                     PGGGKSTATLNTISGAAFVAAASAAPQPAEAQPKEPTALEIAEHLQMSEDALKVAATV
                     KTGPELVAALAGKGLFAKAVRVQAHLLPKRHAVWWGVVCVEPDCADQLPPPERAAYDA
                     AREWVTDPTEANRRRCEAAAGRTKYEAPGSWLAMAAFWSGESLTPADMPAVAPDDKLP
                     GQAVTSSLMIASVFGDSTKGKERYQAFLAKAPQVASGAIPLPEKK"
     gene            13597..14409
                     /locus_tag="VFB80_09020"
     CDS             13597..14409
                     /locus_tag="VFB80_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN33948.1"
                     /translation="MAAGSWLQRACRAIFRERRLPTQLAIARERLWIALGRRCKVISA
                     GELSFCVRRLQSDEFFIRDVVLDREYNPAGYEIGESDIVIDAGGSIGAFAVLAARAAR
                     RGTVVSLEPTRDSFRLLGANLARNNCTSATAVQAALVGTSGPVKIYLSRHSTGGHSVF
                     ADLAGQAESYEMVDGLTLAEVFQRHQLTRCDFLKLDCEGAEFAILDSLPEDLAEQIGK
                     IVVEYHVQPGEPKASQAERLLQRIDRLGFRVERYTDVVDTYWGTIYARRPAQ"
     gene            complement(14430..15419)
                     /locus_tag="VFB80_09025"
     CDS             complement(14430..15419)
                     /locus_tag="VFB80_09025"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN33949.1"
                     /translation="MKTAVFSTKAYDRQFLQPAAAAAGHELTFIESRLTPATAVLARG
                     FPAVCSFVNDALSAEVAGQLAEGGTRFALLRCAGFNQVDLEACGKLGIRVARVPAYSP
                     YAVAEHTVGMMLALNRKYHKAYNRVREGNFAIDSLLGFDMHGKTVGVIGTGKIGAIAA
                     GILHGFGCNLLLADVVPSDECKRIGRYVSLDELYRQSDIITLHCPLTPQTSHLINRQA
                     IEQMKPGVMLINTSRGGLVDTEAVIAGLKSGRLGYLGLDVYEEEAELFFEDLSNRVIQ
                     DDVFMRLLTFPNVLITSHQAFFTDTALAQIAKVTMENLTAFERGAATNEVRRS"
     gene            15493..16467
                     /locus_tag="VFB80_09030"
     CDS             15493..16467
                     /locus_tag="VFB80_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HZN33950.1"
                     /translation="MPPSTLQSGRRTPRPLVAAWILCALAGCAGTRPQQAGPPRPLEA
                     LLAATPPKIELPNWEIPQVDLAQLSADKRVPSNDRDWVPELKVLAYAETDGDKVHIHN
                     VRNAQFFSYRDCLVDYYDKTYDLSKIRTVDFIMIPFADNRAIAHTLLSFGFEGGDYVG
                     VSAEVRLEKGESYGAGTGLFNQFELIYVVADERDLLPVRAEYRNVDVLLYRTKATPDM
                     AKKLFVDVMQRVNQLKEQPEFYDTLSNNCTTNIVRHINTLAPDRIPHDYRVLLPGYAD
                     SLAYELGLIDTSRPFAEVRRNARTNDLILRYKDDPHFSQRIRGEAIVR"
     gene            complement(16543..16872)
                     /locus_tag="VFB80_09035"
     CDS             complement(16543..16872)
                     /locus_tag="VFB80_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZN33951.1"
                     /translation="MRLYVGNLSYSVTSESLEALFAEYGQVKSAQVIQDRDTGRSKGF
                     GFVEMADGAQAQAAIQGLNLKEVDGRCLTVNEARPREERGGGGRGGGGGGGGGRGYGG
                     GGGGRRY"
     gene            17463..21191
                     /locus_tag="VFB80_09040"
     CDS             17463..21191
                     /locus_tag="VFB80_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZN33952.1"
                     /translation="MDVLERTWEGVGNFFGGLMHGFERGVTAVFGSSNARFVRRMQTK
                     VDAINALEPRYQAMSDADLRAETVRFKERLKAGETLDDILIEAFAVGREGGRRFLGMR
                     HYDVQLIGGMVLHSGSIAEMVTGEGKTLVATLPAYLNALSGKGVHVVTVNDYLARRDM
                     EWMGPLYLGLGLTVGAIQSDMQGVEGTLRRQAAYDCDITYGTNNEFGFDYLRDNMRPA
                     ARGDERYSKDRQQSQRKLNFAIVDEVDNILIDEARTPLIISGPSDDNPAKYAEADRIA
                     KLLKKDAHFVVNEKDHTVNLTDEGVRHAEKLAGVESFYTAGNMQWPHLIDNALKAHHL
                     YQKDVRYVVEDDSIIIVDEFTGRKMEGRQWSDGLHQAVEAKEGVKIKEETQTLATITL
                     QNFFKLYDKICGMTGTAMTEASEFWKIYELDVVAIPTNRKMQRKENPDVIYRTEHEKF
                     IALADEIERLNRYDVLVMKDGEELWGTLHKELEDSVEFQKRESKQRDVIPKNKIAEIE
                     RKGRPALVGTVSIEKSERLSSLLDKRGIKHEVLNAKYHKREAEIIAQAGRLGAVTIAT
                     NMAGRGTDIILGGNPETMAWAQLQSKYPTRLDVPPAEWDTLVREIEAREGMKAEGKVV
                     RELGGLHVVGTERHEARRIDLQLRGRCGRQGDPGSSRFFLSLEDDLMRIFAGPWVKTI
                     LETLGMKEGERIESRMVSRRIEGAQKKVEERHFEARKNLLEYDEVMDEQRKRVYTFRQ
                     RILDGASCRGVVLEMLQKEVQQQLDTLLERDYGTGVFAAFASGKLSIDDLKGRDLRGM
                     TFDEAEQYCKDEAQRAAETHVQDGIDENLAGEEGDEESEAEWNWDALAKMGNARWNLS
                     LRDKDLKKLGRSQAGEFLLDQARASIEKADLSEGRPFLDDDFPQRSIVGWLHLKFGIG
                     IDLAEIKGREPAAIRDYVHTLIRNAYDQREAEYPVMAGLYRYTVSTGPQAHLDREGLI
                     AWARERFGADINLESLKNKQRDEIRTVLLQQSLKTQEQANAAVAEASRRVEELFGGAE
                     RTRIARTVAGQNGQLDSLAAWIETSFGRKLTSEEIGELDREKLEQKLIAAIDDKFRPE
                     MRRMERMLLLQIVDHAWMGHLLVMDRLRSSVQLMGYAQVDPKVEFKREGMKLFEQMWR
                     SIGDQATSLIFRMEQLNEEFVHSTWVETSATHAEAPTASQEYQQSQQQTAGAGVDPNA
                     KLEPIRNRGVRPGRNDPCTCGSGKKYKNCCMRKGGPIV"
     gene            21304..22644
                     /locus_tag="VFB80_09045"
     CDS             21304..22644
                     /locus_tag="VFB80_09045"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HZN33953.1"
                     /translation="MPYLLNLAYLLAILVALPWLLYQRLRHGKYREGWSEKFLGRVPR
                     RESNKSCLWLHAVSVGEVNLLAPLLARWERLHPDWECVISTTTQAGYALARKKYAPRS
                     VVYCPLDFTWAVREAVCRIRPALLVLTELELWPNLIAAARRSGAKVAVVNGRLSENSF
                     RGYRRFAWLVGRVLVQLDLVAAQNEEYAERFRALGAPREATYVTGSIKFDGALTRRDA
                     EPVCRLAQLAGIGPHDVVFLAGSTQDPEEALALDVFERLAPAFPELKLILVPRHPERF
                     GEVAALLDRRGVKWVRRSKLYSSPHAPREAIISRSEMTTILVDAVGELGYWWGTAQIA
                     FVGGSLTRRGGQNMIEPAAYGAAVSFGPNTWNFRDAVGQLLDRDAAIVVKSSADLESF
                     VRRCLTDPAFTAQLGRRAQHFVLSQQGAADRTIGLLENLVAETASKEIGRSAAA"
BASE COUNT         4152 a         7524 c         7466 g         3551 t
ORIGIN      
        1 cataatgcgc ataatcgtga cgcggcttgt ttcgacgcaa gttattgcca ctaaatgatt
       61 tgcagcccga aatagcctcc gccagatttg ttttcgatat gcgcgcatga gaacccgggc
      121 tactcctggc cactggccca tggcttctgg cttaccgccc acgcgccaat tcattgtggc
      181 ctttgttgac agctattacg atggaaccgt gatcgttggg gcaatggagc gcccactagc
      241 gcgcgggccg tgccggaaca catggccgcg ggtcagtcag cactttgagg cgcagcgtgc
      301 cgagaaatct cgagcccaga gctggacgac gcgagcgggc cggcgggcag cgtccgctgg
      361 accgcgccgc ctccgcacgc cgccggctcc agctggagcc gctcgaaagc cggagcctgc
      421 tggccgcgct gcccttcggc gcgatgcccg acgacacggg cgagtacatg ctgggcgacg
      481 tgcacgtgac ggtcgtcctg atggaatcga acaacacgat cgacaacggc acgatcacct
      541 atacggatac cgacccaaat accggcggca accagaccgt tcaatacaat tatcccgccg
      601 agaattggac gacccaggcg atcggcgctg tgaaggccaa ggtgcaggcc ggcctggatt
      661 ggtgggagga cacgctcgac gcgctgccga acgtccgcga cggcctgctc aatttcacgg
      721 tcgattggac gtacgccgat acgccggtgg cgaccggcta tgaaccgatc gcgcgagtgt
      781 cgaacgactt tacgctgtgg gtctacgact ttctgaacaa agtcggcttc acccagacgg
      841 gcaatttttc gaccgacatc cgggcctaca acaactcgcg gcgcgaggcg acgggctccg
      901 actgggcctt cacgatcttc gtcgtcaaca acgctgccga cacggacaag ttcttcacgc
      961 aaatcctgct cgactcgggc ggttcgttcg gttcgttcgg ccaggcgttt tcattcgcgg
     1021 gcgggcggtt catgatcgtg ccggccagcc ggccgacggg caccttcgcc cacgaaacgg
     1081 ggcacatgtt ttgggccttc gacgaatacg acggctccag cgcctcgtac cagtcgcggc
     1141 gcggctacta caacacggcg aatagcaacc acgtcaacaa cccgacgccg ggcttcgtgc
     1201 atgccaacag catcatgtcc aacggcgcga gcctcgacgc cgccttcgcc gggcacacgt
     1261 cgtccacgtc gtcgctggag acgatcggct ggaaggactc ggacaacgac ggcatcttcg
     1321 acgtgctcga cgtgccgttc aagctgagcg gcatcggccg ctacaccgcc gccacgggcc
     1381 tgttccgctt cacggggtcg gcttcggtcg ccacgctgcc caaccagaac acaagcggcc
     1441 tgcaaaacga catcacgatc aatcagctgc gggtcgtgga gatggcgatc gacaacggcc
     1501 cgtggaccac ggtgcaaacg ttgccgccgc ggacctatac gacggcgctc gatgtgagct
     1561 ttgccgtgcc cgcgggccag catacggtca agctccgagt ttccgacacg cggaccggcg
     1621 tcacgtcgcc ggagttcatc ggcgacaccg gcgcacccga tgcgatcggc tcgcccggca
     1681 tcggcggctt tgtcttccgc gacgacgacg gcgacggcgt gtgggacgcc aacgagccgc
     1741 cgctggtgga ttatggcctg gatgtcgtcg atcaggacga caccccgatc gacttgcggc
     1801 ggaatctcga gccgagccag ttcccggaat cgaccgtgct gaacgcgatt catccggagg
     1861 ccacgctgac ggcgattggt ggcgacgtgg tcgccgcgac cgagattcag cggctgacca
     1921 tcaccggatc gacgggcagc agtgtcacgc cggcgtttgg gggcgttaac gggttgccgc
     1981 tctcgattca attcggcctc gcgccgaccg cggccgacgt gctggccagc ttgaacgcta
     2041 ttccggctct cgccggcaaa gtcgccgtcg cgggcgctaa cggcggaccc ttcaccgtga
     2101 ctttcagcgg ccagctgagc caggtcaatg tcccgccgat cacgcagggc gcagccagcg
     2161 gcggcgccac gctggccgtc gagtctgtcg tcgaaggcgc gccggctcag gtgatcgccc
     2221 gcaccagcgg cttgtactct agcctggccg gcaaggtctt tacgacgcgc agcatcgcgg
     2281 ctggccagct ggtcgagaag tggactagca gcagccggca gctgaagatc agttttcaga
     2341 acccggtcgg cgtagttagc atccgggcgt ttagcgcgcc gctcgtggcc ggtcagccga
     2401 gtttcgcccg cctggaggct tacaacagcc agggcgtgct gctggagcgg ttcacttcgg
     2461 gggcgatttc cgggaacacc tttgccctgc tgtcgatcaa tcgttcggcc agcgacattg
     2521 cctacgtcat cgcccgcggt cacgtgggaa cggaagtcgt cctggacact ttgcagtggg
     2581 gggcgacgac atccgctacg agcaacccgc taggcgcata ttcgctcgcg ttcctgccgc
     2641 cgggaactta ccgcgtgaag gccgatccgc cgtcgaatca caccgtgacg acgcccgtcg
     2701 gcggcgtggc cacgatcacc gttgcggccg ggcaagcgca gaatgtcaac ttcggcatcc
     2761 gcattcaggc cagcctgtgg cacaacgtcg ccaagccgct caacgttgac aacgatcccc
     2821 tcttggctgt cgcgccgctc gacgcgctgc tcgttatcaa ctggctcaac gcacatccga
     2881 atcagcccga gctgccgtcg caagcgacgc cgtcctccga tggctacatc gacgtcacca
     2941 atgacggcct gtgcacgccg ctcgacgcgc tgctggtgat caacgagctc aactcgggcg
     3001 gcggaggcgg cggaggcgga gggggcggag gcggcggcgg tgagggttgg agcgggggcg
     3061 gtggcggaac tggcggtgga ggtggtggcg aaggcgaaat gcctggctcg catgctcctg
     3121 gcagcgattc gggggacggc gaaggcgaaa tcgctccgag cgcgcccttg agcgccgccg
     3181 aatactacgc ccgcaatccg attcacttcc tccagatcgc cggcgccgat gttccctgcg
     3241 gctgcgagga gtgcctggcc cagcgcgtag cccagccgat cggcgactcg aacgccgcgc
     3301 cggccaaagc aacattgacc gttttcgaca cgctggaatc ggccaaggcc gagccttcgt
     3361 catccagagg cagcgccgtg ccagaggccg atcccacgct gcaggccaaa gttcagcaga
     3421 cgttgctggc cgtcaagaag gccctgctgc cgctcgccgc ccgcaagcag aagctcgagc
     3481 agtcgatcga ctcgatcgcc gccgacgtga gccaagcgca gatcgacgtt ccagcccggc
     3541 tgatgcagcg tcgcggccgc acgcggaatt cctagcccgc cggccgcaaa gttcgcgcag
     3601 cttcgccatc ctcccgtgtc tggcgctctc tggtgcaggg ttgccttaag gggctttccg
     3661 ccgccggtcg ataagctgag aagagtttgc cgctattgcg ctatttgccg attgggcgag
     3721 agttgccgta tgccggactg ccgtttcgcg agcgttgccg tcttgctgct agcggccctg
     3781 gcgggctgcg ccgcagcgcc gcctgcgcct gctccggccg cgcccgcgct gcccacttcg
     3841 gcctcggtcg gcggggcgaa catcgtggcg attgcaccgc ccggtcagca gtgctgcccc
     3901 aagcagacgc tgccgcaatt cctcggcatt gtcggcgcgt gcaatgagat caagaccctg
     3961 tgcaacaaca ttcgcaaccg gctgggtgcg atctggcccg gactggaggc caagccgccg
     4021 gtgctgtcgc tgaccgatcc ggccaatctg cagtcggaca atccggccgt gcaaagcgcg
     4081 gccgaagtca aggctgccga ggacggtgcg gcgcagaagg ccaaagccat cgcctacctc
     4141 gcgagcatcg gctgtgccgg ctgttatccg ggcatcgagg aggcgctgat cgccgcgctc
     4201 gacgactgca ccgagctgat ccgattcgaa acgctcaagg ctctgcggca gcaatcgatg
     4261 ggaccgtgca agaactgctg ccgcagcgcg tgctgcagcc cgaagatgcg cgagaagatg
     4321 gaaaaactgg cctacgagct ggatgcgcag ggctgctacg tcgagccgtc ggagcgtgtg
     4381 cggcggatgg cgcggctagc cctgtgcaat tgccagtgcg tgccgctgaa gccgggccag
     4441 acgccggccg ggctgccgcc ggaaggcccc agccccgccg aggcagcgcc gccacctccg
     4501 cccgcgggcg cgactgcctc ggttctgcgc gcgcttccgg cgacggagca gccgctcggc
     4561 agtggcgtga ttactgcgca gcccgcctcc aggaacacag cgcagcccgc cgctagaaac
     4621 atagctcagc cggctggcgg agcgccggcc cggccggtta gctacgaaga agcactgctc
     4681 agctatcagc ccggcggccg gcaagcagaa ccggccgcgg ccctaacggc ttgggagatc
     4741 tggtcggcgc ggccccagga ttttgcttca cggcccgacg cggttgcggc cttgagcatg
     4801 gcgcggatgc agctgatgac ctcgcaggcc gggccgctgc cgccgcagtt acagatgcag
     4861 acgcacggct ggaccgatcc cgcgacgatt cgctcgccgg cgattgcccg tgcggtggcc
     4921 acgacgccgg ttagcggcgt gagcggcgtg ctggaagatc acctgggatg gcacctcgtg
     4981 cggccgatcg cccgccggcc cgcaccgcgg ccgacgcccg ccgctggaaa ccttgcgcag
     5041 cacgctgcga cacaattacc gtccactgcc acccaactgc aacaacccgc cgctgcgccg
     5101 gttccgcctg cgcttacgac cgttcgcctg ccgccctgcg actgccacta gatccagcgc
     5161 ggcggcggcc gcgcttcggc tacactgacc agcaggggaa tggtgagcga cgccaaggac
     5221 gcccgcatgg ctcaatccac gacaagctcg accgctcctt cggggaatgg gcccaacggc
     5281 cgcaaaggct ggcgcggcgc cgcgcccgcc gaagcgcccc gccatcgctg gcaggtgggt
     5341 gggggcggaa atggcggaag cgtcctcgcc agccggccgt tccgcaaggc gcgcgtcgcc
     5401 gcctggttca tcgccgcggc ggcgctgctg ctctgcctgg cctattacct gctgtatgcc
     5461 ccggcccaga ccccgctctt cgccgtcgtc ggaaccagcc accggcggcc gattccacct
     5521 aatgcctggg ccgccgagga tttgctcgcg ctgcgtgacc tggagggcaa gtccctcgcg
     5581 gtcaccaacc tgtccgaaga ctggcgttcg ccggaccacg ggctcaaacg cctgggggac
     5641 gaactctcgc agtttgttcg ccgccacagc cgcacgcggt cgatcgtcct ctacgtgagc
     5701 ctgccgggcg cggtagacgc cgagggtcgg ccctgcctgc tgacgccgga cgcggcccca
     5761 cacgacagca ccacctggct gccgctggtc aaggttctcg ggcagattca ggcggccgaa
     5821 gtgccggcgg gagttcacaa gctgctggtg ctcgattgca cgcgcgaggt cgccgactgg
     5881 aatctcggcc aggcctacag cgggttcgtc gagtcgctgc ccgcggcgct cgacgaagcc
     5941 aagattccca acctggcgat tctctgctcg gccgggccgg gacagtccgc cgctgtttcg
     6001 ccggccctgc gcggatcggt gttcgggcac tacctccggc tgggcctggc cggcgcggcc
     6061 gacctgcgcg gcttcggcgg caacggcgac catcgcgtga cgctcgcgga gctggagcag
     6121 tacctccgcg gccacgtgca cggctgggcg cggcaccacc tcggccgctc gcaaattccc
     6181 caggtgattc cacaggaggc gaacttcacg gttacgtggt cgctccgccg cggcgatctg
     6241 gaagagctgc gggcgcgatc cggccgcatc gaggccgccg acccgatcgt gcccgccggg
     6301 gaaatcgccc agctctggaa caagctcgat acgctccgcc aggcgcggcc gtggcggttc
     6361 gacccagcgg gctggcgaat gctttcgttc gacctgctcc gcctggaaca gctcaccgcg
     6421 gccgggaagg cctacgaggc cgaagccggt cagctgcgcg gcgaattggc cacgcggctg
     6481 aatcaggccg aagagcgacg tcaggctgca gcccgcagcg gctcgctgcc ggcgtttgcc
     6541 gcgatcctgg acggcgctgc cggtccgccg ctaaagatgc attcgctgtc gatggcggcg
     6601 ctgtggggca cggccgacgc cgacgcgctc cgcgcgctgg gtgcggaaat ggcccgcgtg
     6661 gcagaagagc ccagcccggc caatatcgca gcggcgctcg gtgccggcga agcagccagg
     6721 ctgccggccg cgctcgacga aacgaacctg ctggcaatct ggcagcggct ggctgttccc
     6781 gcggcctggc gcgatcccgc accgctggcg ctggcctggc aagtccattc cctggccgaa
     6841 cagcacgccg caccgctctc gctcgacgga ctgcccggcg acgagcgggc gcatgcctgg
     6901 gtccgcggcc ccttggcgca gctcgacgcg gaccgccgcc agctggaaga cgccgtgctg
     6961 gtcggcccgg cgctggacaa tgccctcgcg cccggccagg acacgcggca ggcactggag
     7021 agcttcacgc tgagcgagcg cgtgaccgcg gcctgcgcgc ttcgcgacga tctgtgggcc
     7081 gagctgccgc acctggccgc ctggctctgc cggccgcagg aactggccga cgacgaagcc
     7141 gcagccgacc gcctgatcga gaagacgctc cggccgctgc tggccgaggc gatccacttc
     7201 gaggagcacc tggagcggac ggggcagggc agggcagggg agctggactg gacgggcgac
     7261 gccctggtcg cctcgatcga tcagaagttc gacgagctgc gcagtaactt tgacgggcgc
     7321 gccagggcgc tggcgaccgc ggtgctggcg accacgaccg agagggcggc cgctgcccgc
     7381 gagatcgacg cggcgctggc cacgccaatc ctccgctggg accagcgcca gcgtttgcgc
     7441 gaaaaacgca gcgaactgca cgtcaagctc gccgccgact acgacgcgat ccaagacgga
     7501 gcagcccagc cggcggcggc tgcggcgccg gcactgccgc acctggtgcg gatgtcgcgc
     7561 tggcagccac acccgctggc tgccttgctc gaccacgcca cggtactcga ctctgctgag
     7621 cggcgagcgg ctgccgagac ggcggaaggc gatcggctac gagcagccct cgagttgaac
     7681 gaggcagcgc gccagcggtt gctcgcgctg tcgcgcgggc cggtcaaatc tctcgtgact
     7741 gggcaggccc aaaacgtgcc cgtcgattgg aagtcgctct gccaggcgga gtcgcggctg
     7801 cgctcagccg cggcgctctg gttcgagccg ccgggcgtcg atccggtgcg ggagctgcgg
     7861 cgagccgact tggcgcagct gtttctgtgg cgagcgcagc gggcgctcga cgactattac
     7921 ggtccggcgc ggcccggtgg cgaggcgttc tttgcggcca gcgccgccga ttacctcgcc
     7981 gcggcccgca gcttcggccc gctcacggaa gcggccgacg cacaggccgc cggcctcgac
     8041 cgcctgctca gccagcggcg gatcgccgcc cgcgaagcgc tcggcgtcac cgcgactgac
     8101 atcctcctgg tcgaacaatt ggcgacggtc gattcgtcgc tcgacgtgcg gacgcggacg
     8161 cccgaagccg ccgccaattt gccgcaagcg acggccgccg tcttcttgtc cgatcagcag
     8221 ggccgcatcg gcccacccgg ctggccgctg gccgtgccgc agcccgatct gaaagcggat
     8281 cagccgctgc tcgcccacaa tatgacgctc gacaccgcgg ggctcaagag ccgcgggccg
     8341 ctggtgcaag cggtggccag cgtgcggggc aacgactttg ccgcgccgct cctcttgcga
     8401 gcgccgggcg gcttgcgggt cgaatatcga ccgatcctct atggcccgcc gcaagtcacc
     8461 gtgctgggct tcgaccgcaa gaaggcttcg gtcgtcttca tcctcgattg ctcgcacagc
     8521 atgagcgcgc cggtcgccgt ggagcggccc gatgaggccg tccgcacgga atcgacgcgg
     8581 atggaagtcg ccaaaggctc gctgcggtcg ctgctggccg aattggcggc ccgtggcgac
     8641 ctgcgcgtcg gcgtgcgcct gttcggccac cgcgtgggct ggagcacgac caaggccgac
     8701 gaattgctgc ggcagactgg ttatgccgag ccgatcccgg cggagctgcg gccctatgcc
     8761 gacgtgcagg aaatcctcga cctgggccgc ttcgacacga cgatcgccgc aaaggtctac
     8821 gaccagttgc agacggtcaa gccgtggggc gagtcgccga tttttctcgc cctgcagcag
     8881 gcgatcgccg actttggcgc ggacgacaat tcggcccgca gcgtggtgct gatcaccgat
     8941 gggcagaaca gccagttcaa tccgccccgc gagttcgcgc cggccctggc cgacgtgctg
     9001 gccgctgccc agcgggcggg cgtcgccatt cacgtcgtcg gcttcgatat cccgaccaat
     9061 gaggccactg cggcccgccg cgacttcgag cagatcgccg cagccagcgg cggctcgttc
     9121 gtgcccgcca aggactcgac cgcgctggtc gagacgctgc agacgctgct gcggccgggg
     9181 cagtttcgcg tggacgacgc cggcggcaac gtgctggccc aggcggaaat cggccgcagc
     9241 atcacgctcc gcaaccatcg cgggcgggaa gactatggcg tggccttcga gaacatgcgc
     9301 gagccggtgg aattgttcgg cggcgaagcg gtggagctga ccgtccgccg cggatcgccg
     9361 cggctggaag tcgtgccgta cctgaagggg aatccgcagc cgcagaacat gatcgtccag
     9421 gaggacgggg cggcgacgcc gctggtggcc tggctgcacc gctcggtgcg gaccgagcag
     9481 gggatccggt tcccgatctc gatccagcat gccgacttgc acttcgtgcc gcggccggtg
     9541 gaaatgtggg tcgaggtcgc gccgctgggc ctgccgccgc aggaggatcc cggcccgtac
     9601 attttctacg acgcgccgct ggaagcggga acgaccgtgc cgctggcgac gttcctggcc
     9661 caaaactggc cggccggcgc gccccgcgcc gagattcgag tctgggccaa atcgaccgcc
     9721 accgagccga ctcaatcgcg gcagctgacg gaagttgcgg atcgcctgcc cacggagggc
     9781 gctggcttcg aagtgccggg cctggccggc gtgacctatc aggtccgcac ggccggcggc
     9841 aacggacagc tgtcggtggg cgtgatcgag cggcacgacg aacgttcgcc cggcgtcggg
     9901 tcgctcaagg tcacgctcgc accgccggcc agccgcgccc tccatcagtt cgacgctgcc
     9961 aaccgcctcg tgctgcacac gttcacctac gagcagccag cggaagacct gcgggggcgg
    10021 ctgtcgatcc agttcacgtc ccgcggcgac gcgctggccc gctcttggcg gaccgcacaa
    10081 ccggcaatcg ttgatgtatc cgaccggctg gacttgctgg aggtcactcc gccggtcggc
    10141 gcaaaccgtt gacggttccg gcgaagagct atactttaac cgcctgactc gcttgtgccg
    10201 ttcgggccgg gcggtgaaac cgggcagagg agacttaaca tggccgacaa attacggcag
    10261 gtgaacgtcc tgctccaggc cctcgcccgc gtgcccgggc tggggttcct ggcccaggcc
    10321 gagtaccgct tgcaggatgc cgtggatgcg gtcgatgatg tgaacgataa tatcgaagac
    10381 aatcagcggt acatggccga cgtcggcaaa gcggtcaccg acatcacgtc gagtgaggac
    10441 gaggagcagc cgtcggaggc gtcggagtcg tcggactctt agcgaggcag gctcgcttcc
    10501 gcgcggcggc tggaagccgc cgccaggcaa ttcgcagcgc gaggcgcaag gcccatggct
    10561 caagccgtca ccgtcaccag tcccttcggc gacggcaagt tgctgttcca gtcgctgacc
    10621 ggccgtgaag aactgggccg gatgttccac tacaagctgg aagcgctcag cgagaacggg
    10681 gcgctggatc tcaacaagat tctcggccag aacatcacga tcaaggccga gttccccgac
    10741 gaccgcgtgc ggtatttcaa cgccgacgtg atccagttcc gccagatcac caagtccgtc
    10801 gataagtact tctgctacga agcgcagctc cgcccgcggc tgtggttcct cacccgcacg
    10861 gccgactgca agatcttcca gaagaagtcg gtgcccgaca tcgtgaaggc gatcctcgac
    10921 gagttgggaa tcaccgacgt caagaagtcg ctgagcaaga cgtacaaccc gcgcgagtat
    10981 tgcgtccagt acaacgagac cgacttcgac ttcatcagcc ggctgatgga ggaagagggc
    11041 atctactact acttcgagca cgccagcggc aagcacacgc tggtgctggc cgattcgctg
    11101 agcgcccacg agagcattac gaagaaagac ccgctgccgt tccggctccg caaggccggc
    11161 tatcaggagg accacatcta cgactggaac atgcagcagc aggtggaatc ggggacgtgg
    11221 gcccacctgg acttcgactt cgtcaagccc cgagccgacc tcaagtcgca gagcgtgatc
    11281 acgcggcagc acacccatgc gagcatggag ctgttcgcgt atcccggcgg ctatttggaa
    11341 aaggccgacg gctcggcgat ggcccgcact cggatcgaag aggtgcaagt gccctgggag
    11401 atgatcagcg gcaacacgac cgcggtcacg atggcgaccg gcaagctgtt caagatgtcc
    11461 ggctacgccc gcgaagacca gaaccgcgag cacctggtca tcgccagcaa tatcgaaatg
    11521 aagatggaga tcgacacggc agcggccgcg ggctcggacg tgctggattc gaaggccggc
    11581 gtgtacttcg actgcaagtt cgaggcgatc ggcagccagc ggccgttccg cacggcctgc
    11641 agaacgccca agccggtcgt gaagggcccg cagacggcgc tggtcgtggg caagagcggc
    11701 gaggagatct ggacggacca gtacggccgc gtgaaggtgc agttccactg ggaccgcctc
    11761 ggcgaaaaga acgagaacag ctcgtgctgg ctccgcgtgg cccagatttg ggccggcaag
    11821 caatggggcg cgatgcacct tccgcggatc ggtcaggagg tgatcgtcga tttcctggag
    11881 ggcgaccccg atcagccgat tgtcaccggc cgcgtctata actcggagca aaaggtgccc
    11941 tacgacctgc cggcgaacca gacgcagagc ggcctcaaga gccgctcgac caaggaagga
    12001 gacgccgaga cgttcaacga actgcggttc gaggacaaga aggacgagga gcagatctat
    12061 ttccacgccc agaagaactt cgaccgcgtc gtcgagaaca acgacacgct gaaagtcggc
    12121 ttcgagaaga aggataaggg cgaccagacg atcgacatct acaacaatcg cacggtgacc
    12181 ctggagaagg ggaacgacaa gctgcaaatc aaggaaggca accgcaccga gttgatcgac
    12241 aaggggaacg acgagctgac catcgcccag ggcaacaaaa cgatcacgat cagccagggg
    12301 aacctgacga tcaagatcga cgccggcaag gcgtcgatcg aagccgccca ggaaatcctg
    12361 ctgaaagtcg gcgagagcac gatcacgatc aagcccagcg ccatcgagtt ggcgagcgtg
    12421 aatgtgaaga ccaccgccac cggcaacttc gaggccagcg gggctcagtc gaaggtcgcc
    12481 ggcagcggca tgctcgacct cgacggcggc acgatcacga tgaattgaca attgacaatg
    12541 caactcacgc tgcaaatcac ggccggcccg catgcgggac gcaaagtgct tctccgcagc
    12601 gggcaagttg cgcgcatcgg ccgcacggag tgggccgact tcagcttccc gcaggatgcc
    12661 gagctggccg atgtccattt cgcggtgcag tgccagctgc acggggccaa ggtccgcaag
    12721 ctggctgccg acaagccgct ggccgtgaac aagcagcaag tcaccgaggc ggagctcaag
    12781 gccggcgaca cgatccaggc cggccagtcg gcgttcgtcg tcgtgttcga cggggcggcg
    12841 gcgcccgggg gcggtaagag caccgccacg ctcaacacga tcagcggcgc ggcgttcgtc
    12901 gccgccgctt cggcagcgcc ccagccggcc gaggcgcagc ccaaggagcc gaccgcgctg
    12961 gaaatcgccg agcatctgca aatgagcgag gacgcactga aggtggcggc cacggtgaag
    13021 accggtccgg agctggtcgc cgcgctggcc ggcaagggat tgttcgccaa ggcggttcgc
    13081 gtgcaggcgc acctgctgcc caagcggcac gccgtctggt ggggtgtggt ttgcgttgag
    13141 ccggactgcg ccgatcaact gccacctcca gagcgtgccg cttacgacgc ggcccgcgag
    13201 tgggtgaccg atccgaccga ggccaaccgc cgccgctgcg aggcagccgc cggccgcacg
    13261 aaatatgaag ctccgggaag ctggctggct atggctgcct tctggagcgg cgaaagcctc
    13321 acgcccgccg acatgcccgc cgtcgctccg gacgacaagc tccccggcca ggccgtgacc
    13381 agcagcctca tgattgcctc cgtcttcggc gattcgacca aaggcaaaga gcgctaccag
    13441 gccttcctcg ccaaagcccc acaagtcgcc agcggtgcga tcccgcttcc cgagaagaag
    13501 tgacgccgag ctccgcggcc gtgcggcgcg gcattgcaag ttcctctaaa atggtccggc
    13561 atggaaccgg aacgcatctt tccggcgccc cctgtaatgg ccgcgggaag ctggctccag
    13621 cgcgcctgcc gggccatctt tcgcgagcgg cggctaccga cacaactggc gatcgctcgc
    13681 gagcggctgt ggattgccct gggtcggcgc tgcaaggtga tcagcgccgg cgaactttcc
    13741 ttctgcgtgc ggcgactgca gtcggacgaa ttcttcattc gcgacgtcgt gctcgatcgc
    13801 gagtacaacc ccgccggcta tgagatcggc gagtccgata tcgtcatcga cgcgggcggc
    13861 agcatcggcg catttgccgt gctcgcggcc cgggccgcca ggcggggcac ggtcgtctcg
    13921 ctcgaaccga cgcgcgacag ctttcgactg cttggcgcca acctggcgcg caacaactgc
    13981 accagcgcca cggccgtgca ggcggcgctc gtcggaacaa gcggaccagt caagatctat
    14041 ctcagccggc acagcacggg aggccattcc gtgtttgcag atttggcggg ccaggcggaa
    14101 agctatgaaa tggtggatgg ccttacgctg gccgaagtct ttcaaaggca ccagttgacc
    14161 cggtgcgact tcctcaagct ggactgcgag ggtgccgagt ttgccatcct cgattccctg
    14221 cccgaggatc tcgcggagca gatcggcaag atcgtcgtcg agtatcacgt tcagcccggc
    14281 gagcccaagg cctcgcaggc ggagcgcttg ctgcaacgaa tcgaccggct gggcttccgc
    14341 gtcgaacgct acacggacgt cgtggacacc tattggggca cgatctacgc gcggcggccg
    14401 gcccagtaac caaagttgcc ggaatttggc tacgaacgac gcacctcgtt cgtcgccgcg
    14461 ccgcgctcga aggcggtgag gttttccatc gtgaccttgg cgatctgcgc cagagcggtg
    14521 tcggtgaaga atgcctggtg cgacgtgatc agcacgttgg gaaacgtcaa gagccgcatg
    14581 aacacgtcgt cctggatcac gcgattggaa aggtcctcga agaacagctc ggcctcttct
    14641 tcgtagacgt cgaggcccag gtagccgagc cgcccgcttt tgaggccggc gatcaccgcc
    14701 tcggtatcga ccaggccccc gcggctggtg ttgatgagca tcacaccggg cttcatctgt
    14761 tcgatggcct gccggttgat caggtggctg gtctgcggag tgagcgggca gtggagcgtg
    14821 atgatatcgg actgccggta gagctcgtcg agcgaaacgt agcggccgat ccgcttacac
    14881 tcatcgctgg gaaccacgtc ggccagcagc aggttacagc cgaagccgtg caggattccc
    14941 gccgcgatcg cgccgatctt gcccgtgccg atgacgccaa ccgttttgcc gtgcatgtcg
    15001 aagccgagca ggctgtcgat cgcaaagttg ccttcgcgga cgcggttgta ggctttgtgg
    15061 tacttgcggt tcagggcgag catcatgccg accgtatgct cggcgacggc gtagggcgaa
    15121 taagccggca cgcgggccac gcggatgccg agcttcccgc aggcctccag atcgacctgg
    15181 ttaaagccgg cgcagcggag gagggcgaac cgcgtgccgc cttcggccaa ttggcctgca
    15241 acctcggccg acagcgcgtc gttgacgaac gagcagaccg ccggaaagcc gcgggccagc
    15301 accgcagttg ccggagtcag ccgcgattcg atgaacgtca gctcatggcc cgccgcggct
    15361 gccgcgggct gcaggaactg gcggtcgtag gccttggtgc tgaagacggc ggttttcata
    15421 cgtaccgtgt tttgtgcttg gtgtttgggg ttgccccaaa ttgcccagct tggtattcac
    15481 cgcatcgatt ctatgccgcc gtctaccctt cagtcaggca ggagaacacc gcgaccactg
    15541 gtcgcggctt ggatactttg cgcgctggcc ggctgtgcgg ggactcggcc gcagcaggcc
    15601 ggaccgccgc ggcctctgga agcgctgctg gcggccacgc cgccgaagat cgagctgccc
    15661 aactgggaga ttccgcaagt cgatctcgcg cagctctcgg ccgacaagcg cgtgccttcg
    15721 aacgaccgcg actgggttcc cgagctaaag gtcttggcct atgccgaaac ggacggcgat
    15781 aaggtccaca tccacaacgt ccgaaacgcg cagttcttca gctaccgcga ctgcctggtg
    15841 gattactacg acaagacgta cgacctgagc aagattcgca ccgttgactt catcatgatc
    15901 ccgttcgccg acaatcgggc catcgcccac acgctgctca gcttcggctt cgaagggggc
    15961 gactacgtcg gcgtctcggc ggaagtgagg ctggaaaaag gcgagtcgta cggcgcgggc
    16021 acgggcctgt tcaaccagtt cgagctgatt tacgtcgtcg ccgacgagcg ggacctgttg
    16081 ccggtgcggg ccgagtaccg caacgtcgat gtgctgctct accgcacgaa ggccacgccc
    16141 gatatggcca agaagctgtt cgtcgatgtg atgcagcggg tcaaccagct caaggagcag
    16201 ccggagtttt acgacacgct cagcaacaac tgcacgacga acatcgtccg gcacatcaac
    16261 acgctggcgc ccgaccgcat tcctcacgat taccgcgtgc tcctgcccgg ctatgccgac
    16321 agcctggcct acgagctggg gctgatcgac acctcgcgcc ccttcgccga agtccgccgc
    16381 aatgcgcgga ccaacgacct gattctgcgg tacaaggacg acccgcactt ctcgcagcgg
    16441 atccgcggcg aggcgatcgt ccggtagtct ggtccatttg gctgccaaga aagaaaagag
    16501 gcagacaaga atgtctgcct cacgtcattt ctgcgcctcg gattagtacc gccggccgcc
    16561 gccaccgccg ccgtagccgc ggccaccgcc gccaccgcca ccgccaccgc ggccgccgcc
    16621 gccgcgctct tcgcgcgggc gggcttcgtt cacggtcaag cagcgaccat cgacttcctt
    16681 caggttgagt ccctgaatcg ccgcctgggc ctgggccccg tcggccattt ccacgaagcc
    16741 aaaacccttg ctccggcccg tgtcgcggtc ctgaatgacc tgcgcactct tcacctggcc
    16801 atactcggcg aacagcgcct ccaggctttc actggtgacg ctgtagctca agttaccgac
    16861 atacaatcgc attcccacgg cagaattcct gatgcagttg cccggcccga tcttgctttc
    16921 cgggccgatt ggaaagggac caaaaatggt ggtccgccaa gcaggcaagg cgagaagggc
    16981 tgcgaccgat tcaggagaat gcacgctgca cttgcgtcgt ccaacccgac cggggtggat
    17041 tatagcagcc gcaggcggtg gggccagtcc agtgtccgta gtcagctgtc ggttgtaaat
    17101 tgtgccggcc gaatccaact gacaactagc cactgaccct ctcacaaagc cccaaatagc
    17161 agccgcttgc gggcgaactg tctttctggc agccggccag ctaacaactc cgaaatccac
    17221 cccccaggac cgcaattgac cgtttcctgc cgtcgcaata aacttttgtg gcggcggggt
    17281 ttgcgccgat gcggcaggcg gcggacccct gccgggctcg gtggcggaac gctggttgca
    17341 cctgaccggc cgccggaaga aagtgcgcac ttgctgcgga agtcttggca gccggcaatc
    17401 gccggtttgg agcgctgacg gccggcggcc cgcggctttg gaacacaaga gcatagccag
    17461 ttatggacgt acttgagcgg acctgggaag gcgtcggcaa ctttttcggc ggcctgatgc
    17521 atggcttcga gcgcggcgtg accgccgtct tcggctcgtc caacgcccgc ttcgtccgcc
    17581 gcatgcagac caaggtggac gcgatcaacg ccttggagcc gcggtaccag gcgatgtccg
    17641 acgcagacct gcgcgccgaa acggtccgct tcaaggaacg tctcaaggcc ggcgagacgc
    17701 tggacgacat cctgatcgag gcgtttgccg ttggccgcga agggggccgg cgattcctcg
    17761 gcatgcggca ctacgacgtg cagctgatcg gcggcatggt gctgcacagc ggctcgatcg
    17821 ccgaaatggt gacgggcgaa ggcaagacgc tggtggcgac gctgccggcg tatctgaatg
    17881 cgctgtcggg caaaggcgtg cacgtcgtca cggtcaacga ctacctggcc cgccgcgaca
    17941 tggagtggat gggaccgctg tatctcggct tggggctgac cgtcggcgcg atccagagcg
    18001 acatgcaagg cgtcgagggc acactccgcc gccaggcggc ctacgactgc gacatcacct
    18061 acggcacgaa caacgaattc ggcttcgact atctccgcga caatatgcgg ccggccgccc
    18121 gcggcgatga gcggtactcc aaggaccggc agcaatcgca gcggaaactg aatttcgcga
    18181 tcgtcgacga ggtcgataac atcctgatcg acgaagcccg cacgccgctg atcatcagtg
    18241 gtccgtcgga cgacaatccg gccaagtacg ccgaggccga ccgcatcgcc aagctgctga
    18301 agaaggacgc gcacttcgtc gtcaacgaga aagaccacac ggtcaacctg accgatgaag
    18361 gcgtgcggca cgccgagaag ctggcgggcg tggagagctt ctacacggcc ggcaacatgc
    18421 agtggccgca cctgatcgac aacgccctca aggcccacca cctctatcag aaggacgtgc
    18481 ggtacgtcgt cgaggacgac tcgatcatca tcgtcgatga attcaccggc cgaaaaatgg
    18541 aaggccgcca gtggagcgac ggcctgcacc aggcggtcga agccaaagaa ggcgtgaaaa
    18601 tcaaggaaga gacgcagacg ctggccacga tcaccctgca gaacttcttt aagctctacg
    18661 acaagatctg cggcatgacg ggcaccgcca tgaccgaggc cagcgagttc tggaagatct
    18721 acgagctgga cgtcgtcgcc atccccacca accgcaagat gcagcggaag gaaaaccccg
    18781 acgtcatcta ccgcaccgag cacgagaagt tcatcgccct ggccgacgag atcgagcggc
    18841 tgaaccgcta cgacgtgctc gtgatgaagg acggcgagga gctgtggggc acgctgcaca
    18901 aggagctgga ggacagcgtc gagttccaga aacgcgaaag caagcagcgc gacgtcattc
    18961 ccaagaacaa gattgccgag atcgagcgca agggtcgccc ggccctcgtc ggcacggtct
    19021 cgatcgagaa gagcgagcgg ctctccagcc tgctcgacaa gcggggcatc aagcacgagg
    19081 tcttaaacgc caagtatcac aagcgcgagg ccgagatcat cgctcaggcc ggccgcctgg
    19141 gcgcggtgac tattgctacg aacatggccg gccgcggcac cgacattatc ctcggcggca
    19201 atcccgagac gatggcctgg gcccagcttc aaagcaagta tccgacgcgg ttggacgtgc
    19261 cccctgcgga gtgggacacg ctcgtccgcg aaatcgaggc ccgcgaaggc atgaaagccg
    19321 agggcaaggt cgtccgcgag ctgggcggcc tgcacgtcgt gggcaccgag cggcacgagg
    19381 cccgccgcat cgacctgcag ctccgcggcc gctgcggccg ccagggcgat ccgggcagca
    19441 gccggttctt cctgtcgctc gaagacgacc tgatgcggat tttcgccggg ccctgggtca
    19501 agacgattct cgagacgctc ggcatgaagg agggcgagcg gatcgaaagt cggatggtca
    19561 gccggcggat cgaaggcgcc cagaagaagg tagaagagcg gcacttcgag gcccgcaaga
    19621 atctgctcga gtacgacgag gtgatggacg agcagcggaa gcgggtctat acgttccgcc
    19681 agcggattct cgacggcgcg agctgccgcg gcgtcgtgct cgagatgctg cagaaggaag
    19741 tgcagcagca gctcgacacg ctgctggagc gcgattatgg gactggtgtc tttgccgcat
    19801 tcgccagcgg caagctcagc atcgacgatc tcaagggccg cgacctgcga ggcatgacgt
    19861 tcgatgaagc cgagcagtat tgcaaggacg aggcccagcg ggcggccgag acgcacgtcc
    19921 aggacggcat cgacgaaaac ctggccggtg aggaagggga cgaagagtcc gaggccgagt
    19981 ggaactggga cgcactggcc aagatgggca acgcccgctg gaatttgtcg ctccgcgaca
    20041 aggatctcaa aaagcttggc cgcagccagg cgggcgagtt cctgctcgac caggcgcggg
    20101 cttcgatcga gaaggccgac ctgtccgagg gccggccttt cctggacgac gattttccac
    20161 agcggtcgat cgttggctgg ctgcacctca agttcgggat cggcatcgac ctggccgaaa
    20221 tcaagggccg cgagccggcg gcaatccgcg actatgtgca cacgctgatc cgcaacgcat
    20281 atgaccagcg cgaggccgag tacccggtta tggccggcct ctaccgctac accgtcagta
    20341 ccggcccgca ggcccacctc gaccgtgaag ggctgatcgc ctgggcccgc gagcgttttg
    20401 gcgcggacat caacctggag agcctcaaga acaagcagcg cgacgagatt cgcacggtcc
    20461 tcttgcagca gagcctgaag acgcaggagc aggccaacgc cgcagtcgcc gaagccagcc
    20521 ggcgagtcga ggagctgttt ggcggcgccg agcggacgcg gatcgcccgc acggtcgccg
    20581 gccagaacgg ccagctcgac tcgctcgccg cctggatcga gacgtcgttc ggccgcaagc
    20641 tgaccagcga ggagatcggc gagctcgacc gcgaaaagtt ggagcagaag ctgatcgccg
    20701 cgatcgacga caagttccgc cccgaaatgc ggcggatgga gcggatgctg ctcttgcaga
    20761 tcgtcgatca cgcctggatg ggccacctgc tggtgatgga ccgcctgcgg agcagcgtgc
    20821 agctgatggg ctacgcccag gtcgatccca aggtcgagtt caagcgcgag ggcatgaagc
    20881 tcttcgagca gatgtggcgc tcgatcggcg accaggccac cagcctgatc ttccgcatgg
    20941 agcagctcaa cgaggaattc gtacattcca cctgggtcga gaccagcgcc acgcacgccg
    21001 aagccccgac cgcctcgcag gaataccagc aaagccagca gcagaccgcc ggcgcgggcg
    21061 tcgatcccaa cgccaagctg gagccgatcc gcaaccgcgg cgtccggcca ggacgcaacg
    21121 acccctgtac ttgcggcagc ggcaagaagt acaagaattg ctgcatgcgc aaaggcggac
    21181 cgatcgtata ggagtgtcag ttgtcagtag tcagtagtca gtagtcagta gtcagtagtc
    21241 agtggtcagt tgtcgtcgca atctgctggc aactgacaac tgaccgaaca aggatgttcg
    21301 acgatgccct acctcctcaa cctcgcctac ctgctggcga tcctcgtcgc gctcccctgg
    21361 ctgctctacc agcggctccg ccacggcaag taccgcgaag gatggagcga gaaattcctg
    21421 ggccgcgtgc cgcgccgcga aagcaataag agctgcctct ggctccacgc ggtgagcgtc
    21481 ggcgaggtca acctgctcgc gccgctcctg gcccgctggg agcggctgca tcccgactgg
    21541 gaatgcgtca tctcgacgac cacgcaggcc ggctacgccc tggcccgcaa aaagtacgcc
    21601 ccgcgatcgg tcgtctattg cccgctcgac ttcacctggg cggtccgcga ggcagtctgc
    21661 cgcatccggc cggcgctgct ggtactgacc gagctggagc tgtggcccaa cttgattgcc
    21721 gccgcccggc gcagcggcgc gaaggttgcc gtcgtgaatg gccggctcag cgaaaacagc
    21781 tttcgcggct accggcgctt cgcttggctc gtcggccgcg tgctcgtgca gctcgacctc
    21841 gtcgccgccc agaacgagga atacgccgag cgatttcgcg cgctcggcgc tccgcgcgaa
    21901 gcgacgtacg tcaccggctc gatcaaattc gacggcgcgc taacccgccg cgacgctgag
    21961 ccggtttgcc gcctggcgca gcttgccggt atcggcccgc acgacgtcgt gttccttgcc
    22021 ggcagcactc aggacccgga agaggcgctg gcgctggatg tcttcgagcg gctcgcgccg
    22081 gcgtttccgg aattgaagct gattctcgtc ccgcggcacc ccgaacggtt tggcgaagtt
    22141 gctgccctgc tagaccgacg cggcgtgaag tgggtgcgga ggtcgaagct ttatagtagt
    22201 cctcacgctc cgcgtgaggc aatcatctcg cggagcgaga tgactactat tctcgtcgat
    22261 gccgtcggcg agctcggcta ctggtggggc accgcccaga tcgcgtttgt cggcggcagc
    22321 ctcacccgcc gcggcggcca gaacatgatc gagcccgccg cctacggcgc agccgtcagc
    22381 ttcgggccca acacctggaa cttccgcgac gccgtcggcc aattgctcga tcgcgatgcc
    22441 gcgatcgtcg tcaagagctc agccgacctc gaatcctttg tccgccgctg cctgaccgac
    22501 ccagctttca ccgcccaact cggccgccgc gctcagcatt ttgtcctctc tcaacaaggc
    22561 gccgccgacc gcactattgg cctgctggaa aaccttgtcg cggaaaccgc atccaaagaa
    22621 atcggccgct ccgccgcggc atgaaaaaag ccgccgtatc actacggcgg ctaagtaccc
    22681 cctaggggat ttg
//
LOCUS       DASRJB010000341         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3762470, whole genome shotgun sequence.
ACCESSION   DASRJB010000341 DASRJB010000000
VERSION     DASRJB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3762470"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1543)
                     /locus_tag="VFB80_09050"
     CDS             complement(74..1543)
                     /locus_tag="VFB80_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN33954.1"
                     /translation="MNARRLALVLTLAAGAVLGGWQPPLRAADLPARPNIVLILADDL
                     GYGDLGCYGQKRIQTPRIDRLAKEGLRFTDFYAGCTVCAPSRCVLMTGYHTGHAQIRG
                     NGASEIQVLREQDLTAAEVMKKAGYATALCGKWGLGDEGSIGAPNKQGFDYFFGYLNQ
                     HHAHNYYPAYLFKNERRFPLKNVVPGEGDFGSGVASQKVEYSPDLITAEALRWVLEHV
                     KEHKDQPFFLYYAPTIPHANNEGRQQGMEVPDLGQYAATDWPANQKAHAAMISRLDSD
                     VGKLLDLLARLGLGEKTLVLFSSDNGPHQEGGNDPHFNDSNGPLAGIKRDLTEGGIRV
                     PFIARWPGQIAPGTTSSFVGGFQDFLPTAAELAGAASSVPSDCDGVSIVPTLRGSSSQ
                     QKQHDFLYWTFYERGGGRAIRAGDWKLVQQPLSSSERLYNLAQDIGEERDLAADQPQL
                     VARLAAKMESAYVPHENWKFPSAAGKAKAGGKQKAKSPQ"
     gene            complement(1654..>2127)
                     /locus_tag="VFB80_09055"
     CDS             complement(1654..>2127)
                     /locus_tag="VFB80_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33955.1"
                     /translation="SGTVWSLGPGGQAQGQIGRWEQQGDRVRFQWAASSQGFDVQFQQ
                     QPYISSPDIYLFSYLGRGSSRRTSNSFGPDRALVGPQRQIYGRSNALPAGPVVARGDV
                     ALDLRTFTGQSIDGVWRPIEIVGNPRLTQPIRHVPGQWQSVRFIIDGGTPPRYGR"
BASE COUNT          348 a          721 c          682 g          376 t
ORIGIN      
        1 tgccggcaaa cagcgcggcg aaaactccgt gcggcggggc ccatcccgaa tgcgaatagg
       61 ttttcatagc tggctattgc ggcgacttcg ctttttgctt cccgccggcc ttggccttac
      121 ccgcggccga cgggaacttc cagttttcgt gcggcacgta agctgattcc attttagctg
      181 cgagccgggc gacaagctgt ggctgatccg cggccaggtc gcgctcctcg ccgatgtcct
      241 gggccaggtt gtagagccgc tcggacgagc tcagcggctg ctggaccagc ttccaatcgc
      301 ccgcgcggat cgcccgtccg ccgccgcgtt cgtagaaagt ccaatacaga aagtcgtgct
      361 gcttttgctg gctggaactg ccgcggagcg tgggcacgat ggaaacgccg tcgcagtcgc
      421 tcggcacgct gctcgcagcg ccggcaagct ccgccgcggt gggcaggaaa tcctggaagc
      481 cgccgacgaa gctgctggtt gtgcccgggg cgatctggcc cggccagcga gcgatgaagg
      541 gtacgcggat gccgccttcg gtcagatcgc gcttgatgcc cgccagcggg ccgttgctgt
      601 cgttaaagtg aggatcgttg ccgccttcct gatgcgggcc gttgtcggac gagaacagca
      661 ccagcgtctt ctcgccgagg cccagccgcg ccagcaggtc cagcagcttt ccgacgtcgc
      721 tgtccagccg cgaaatcatg gcggcgtgcg ccttctgatt cgcgggccaa tcggtagctg
      781 cgtactggcc gagatcgggc acctccatgc cttgctgccg gccctcgttg ttggcgtgcg
      841 gaatggtcgg cgcgtaatag agaaagaacg gctggtcctt gtgctccttg acgtgctcca
      901 agacccagcg cagggcctcg gccgtgatca ggtccgggct gtactcgacc ttctggctcg
      961 cgacgccgct gccgaaatct ccctcgcccg gcacgacgtt cttcagcgga aaccgccgct
     1021 cgtttttgaa caggtacgcc ggatagtaat tgtgggcgtg atgctggttg aggtagccga
     1081 agaagtagtc gaagccctgc ttgttcggtg cgccgatcga gccttcatcg cccaggcccc
     1141 acttgccgca cagcgcggtg gcatagccgg ccttcttcat gacttccgcc gcggtgaggt
     1201 cctgctcccg cagcacctgg atctcgctcg cgccgttgcc gcgaatctgg gcgtgacccg
     1261 tgtgatagcc ggtcatcaag acgcaccgcg aaggggcgca gaccgtgcag cccgcataga
     1321 agtcggtgaa ccgcagtccc tccttcgcca gccgatcgat ccgcggcgtc tggatccgct
     1381 tctggccgta gcaacccaga tcgccatagc ccaggtcgtc cgccaggatc agcacgatgt
     1441 tgggtcgcgc cgggagatcg gccgcccgca gcggcggctg ccagccgccg agaaccgctc
     1501 ccgccgccaa cgtaagaacc agcgccaatc gtcgagcatt cacgctgcaa ctcccgaccg
     1561 gtgcacgatt ggcccggcga accccagacg agcccacgca ttgtcccatc gctcgccttg
     1621 gccgtcaaat cggccgcgga gtcctaagcg tccctagcgg ccgtaccggg gcggcgtgcc
     1681 gccgtcgatg ataaaccgca cgctctgcca ctggccgggg acgtgccgga tcggttgggt
     1741 cagccgcggg ttgccgacga tttcgatcgg ccgccagacg ccgtcgatgc tttgcccggt
     1801 gaaggtccgc aggtcgagcg cgacgtcgcc gcgggcgacg accggaccgg ccggcaaggc
     1861 gttcgatcgc ccatagattt ggcgctgcgg ccccacgagc gcacggtccg ggccgaacga
     1921 gttgctcgtc cgtcgcgacg acccgcggcc gagatacgaa aacaaataaa tgtcggggct
     1981 cgagatataa ggctgctgct gaaactgcac atcgaatccc tgggaggagg cagcccactg
     2041 aaaccggacg cggtcgcctt gctgctccca cctgccaatt tgcccctgcg cctgcccgcc
     2101 cggccccagg ctccaaacgg tccccga
//
LOCUS       DASRJB010000342         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2635523, whole genome shotgun sequence.
ACCESSION   DASRJB010000342 DASRJB010000000
VERSION     DASRJB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635523"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFB80_09060"
     CDS             <1..1158
                     /locus_tag="VFB80_09060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN33956.1"
                     /translation="FIPNRWQRAAVVLGVMSAAPPLGAIATALAGGHSGGLLATDARV
                     IVEMVLILSLAFAAGVLGVHTINALRSEVFRARQLGQYKLKRLIGSGGMGDVYLAEHL
                     LMKRPCAIKVIRPEKAGDPKVLARFEREVQATAKLSHWNSVDIFDYGRADDGTFYYVM
                     EFLPGMNLSELVRRYGPMPTSRALHLVRQVCDALQEAHHLGLVHRDLKPANIFAAARG
                     GLFDVAKILDFGLAKPLTDLAAAPLTQEGTITGSPLYMSPEQAIGDRDPDSRSDIYAL
                     GAVLYFLLTGRTPFADENPMKVLIAHAHEPPPPPSQHGEVPDDVELIIMACLQKSPDD
                     RYQSAAELAAAIEECDDFGRWTRESARAWWQEHETQHVREHAEELSVGMQS"
     gene            complement(1173..2279)
                     /locus_tag="VFB80_09065"
     CDS             complement(1173..2279)
                     /locus_tag="VFB80_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33957.1"
                     /translation="MSCMCVNQLAAIAAQLKNLPLAMLMELLGPMRMASAGLSRLSLA
                     ASASASASASASATASLNASLSAALAANMNLSASAVAKLEALASVCRTMGLSPFGLSA
                     VASLNVAATSMNASIPTLSAMLSALLSPAAAALAELLKLGSSLNMLGGMTGMNLALPG
                     VGASLGATAAAALSASVSASASASASASASLSASAALALRLSAAATALGINLGLPGGA
                     AQLNAALSLAAKISPPAFGLGALPQLSAQMSALSLANTALGVDLLKSMALAQLQAALG
                     NISANVSAAFAAAASASASASASASASLAASAAASANLSAAASAAAQLNAAAMLGLPN
                     LGPMSMAVNLNAQLALATGINPMAAVPCPSCSFF"
     gene            2494..2855
                     /gene="ssrA"
                     /locus_tag="VFB80_09070"
     tmRNA           2494..2855
                     /gene="ssrA"
                     /locus_tag="VFB80_09070"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          516 a         1012 c         1038 g          485 t
ORIGIN      
        1 ttcatcccga ataggtggca gcgggccgcg gtcgtcctgg gcgtgatgtc ggccgcgccg
       61 ccgctggggg cgattgccac ggcgctggcc ggcgggcaca gcggcggcct gctggcgacg
      121 gatgcccgcg tgatcgtcga aatggtcctg attctctcgc tggcgtttgc tgccggcgtg
      181 ctcggcgtgc acacgatcaa cgccctccgc agcgaggtct ttcgcgccag gcaactcggc
      241 cagtacaagc tcaagcggct gatcggcagc ggcggcatgg gggacgtcta tctggccgag
      301 cacctgctga tgaaacggcc ctgcgctatc aaggtgatcc ggccggaaaa ggccggcgat
      361 cccaaggtgc tggcccgctt cgagcgcgag gtgcaggcga ccgccaagct ctcgcactgg
      421 aacagcgtgg acatcttcga ctacggccgg gccgacgacg gcacgttcta ttacgtgatg
      481 gagtttctgc ccgggatgaa cctgagcgag ctcgtgcggc ggtacgggcc gatgcccacg
      541 tcccgcgcgc tgcacctggt gcggcaggtc tgcgacgcgc tgcaagaggc ccaccacctg
      601 gggctcgtgc accgcgacct caagccggcg aacatcttcg cggccgcgcg gggcggactc
      661 ttcgacgtgg ccaaaatcct ggattttggc ctggccaagc cgctgaccga tctggccgct
      721 gcgccgctca cgcaggaggg cacgatcacc ggctcgccgc tctatatgtc gccggagcag
      781 gcgatcggcg accgcgaccc cgactcccgc agcgacatct acgccctggg cgcggtgctc
      841 tatttcctgc tcaccggccg cacacccttc gccgacgaga acccgatgaa ggtgcttatc
      901 gcccacgccc acgagccgcc gccgcccccc tcgcagcacg gcgaagtgcc cgacgacgtg
      961 gagctgatca tcatggcctg cctgcagaag agccccgacg accggtatca gtccgccgcc
     1021 gaattggccg ccgccatcga ggaatgcgac gacttcggcc gctggacccg cgagtccgcc
     1081 cgggcctggt ggcaggaaca cgagacccag cacgtccgcg agcatgcgga agagctgtcc
     1141 gtggggatgc aatcgtaatt cgtcgctccg agctaaaaga acgagcacga cgggcagggc
     1201 acggcggcca tggggttgat gccggtggcc agggccagct gggcgttcag gttgacggcc
     1261 atcgacatcg gccccaagtt gggcagtccc agcatcgccg cggcgttgag ttgggccgct
     1321 gccgacgccg cagccgagag gttcgcgcta gccgccgcgc tcgcggctaa gctcgcactc
     1381 gccgaggccg aagccgacgc gctggcgctg gccgcagctg caaacgcggc gctgacgttg
     1441 gccgagatgt tccccaacgc ggcctggagc tgcgccaggg ccatgctctt gagcagatcg
     1501 acgcccagcg cggtgttggc cagcgacagc gccgacattt gagccgacag ctgcggcaaa
     1561 gcgccgagcc caaacgccgg cggcgagatc ttggcggcca gcgacagcgc cgcgttgagc
     1621 tgggcagccc cgcccggcag ccccaggttg atgcccagcg ccgtggcggc agcgctcagc
     1681 cgcagggcca gcgcggcgct ggccgacagg ctggccgagg ccgaagccga cgcggaagcg
     1741 gatgcggata ccgacgccga cagcgccgcg gcagccgtcg cgcccaggct cgctccgacg
     1801 cccggcagcg ccaggttcat gcccgtcatt ccgcccagca tgttcagcga cgagcccagc
     1861 ttgagcagct cggccagtgc agcagccgcg gggctcagca gcgcggagag catcgccgag
     1921 agggtgggga tgctggcgtt catcgacgtg gcggcgacgt tcaggctggc aaccgcactt
     1981 aagccgaatg gcgagaggcc catcgtgcgg cagacgctcg ccagggcttc cagcttcgcc
     2041 accgccgaag cggaaaggtt catgttcgcg gccaaggccg cgctgaggct ggcattgagg
     2101 ctggccgtgg ctgatgccga ggccgacgcg ctggccgagg cgctggcggc caggctcagc
     2161 cgcgagagcc cggcgctggc catccgcatc ggccccagca gctccatcag catcgccagg
     2221 ggcagattct tgagctgtgc cgcgatggcg gcgagctgat tgacgcacat gcaactcatg
     2281 gtcggtcccg gcggaggtca ggcggccgaa tggtagcgtt ttgctgttcc gcggccaacc
     2341 tgattgactg ttcggatcgt aactactctc ccacgcggga attctccaga ggccggcctg
     2401 cgtcccttca ccccatgcag gctggcgcct agaattgccg atgaaccttt tggtttcctg
     2461 gctgctggcg actggctcct ggtcactgac tttgggggcg aactggattc gaccggatgc
     2521 ttggaaacgc aagtggcgtg tcgtggttga tcagttggcc acgttaaaag ctgatcacaa
     2581 tgttacttgc cgagagcaat ctcgccctgg ctgcctagct taagacggta gcctcggact
     2641 gcgggtcttc gccggagaga cccaaaagcc cgtagccaat tccggaccgc tgcgagtcac
     2701 gtggggtacg ctcgcggtta aacaagttct ccaatagccg ctccggtagt ctgccgtgaa
     2761 acgcccggac ggtgaagtta aagtcacggc tacacacgta gaagcctgcg tggacagatc
     2821 tcgggacgcg ggttcaattc ccgccgcctc cactttgcca gttggcgaca actggcgacg
     2881 atcaacgtca aaacccttgc cctggcaagg gttttggcat ttcttgaccg ctggttcggt
     2941 gccaggttgc gacatcgaat gaccatcaac gccgcctgaa atgcagcagc gcgctgcccg
     3001 gcggggggga tgccggggcg gcgggtttga gcgacggcag cttggccagc g
//
LOCUS       DASRJB010000343         5745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_97404, whole genome shotgun sequence.
ACCESSION   DASRJB010000343 DASRJB010000000
VERSION     DASRJB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97404"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="VFB80_09075"
     CDS             <1..2292
                     /locus_tag="VFB80_09075"
                     /inference="COORDINATES: protein motif:HMM:NF037833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33958.1"
                     /translation="EVGTAGAGATTFIDTNPDGQGVPAGTYYYRVEAFAPGLPDSAFS
                     NVDSVRFAAPGLPLTIDHSEGFASHGDLTTSGSTTIFPQPAPVGTFLGHQDLGGVAAP
                     GTATFDGGLYTLRASGSDIWDVADSFQYVYKPLAGDGTIEARVVGVQNTDFWAKAGLM
                     IRENLRANSKNAFMFATPPGHDEPVFQFRADTGGFSADYGNHISGLQAAPVWLRLVRQ
                     GNSFSGFWAQDLGGGMHGPWNQIGQDTTINMAGTVYVGLALTAHNNSGVLNTSTFDHV
                     TITGATAPLPPTVVELTDGGFGEAGGAFLSNRVGIEDFTSTFTFQITPGTSPMADGLA
                     FVIQGLGPTALGPTGGGLGYGSDFVGGGGGLGRSLAIKFDIFNNSGEGINSTGIFTNG
                     RSPTVRQPGLAGGFPDTSINLDGTGINLASTHPFRVTLSYDGRALTETITDTVTSATF
                     TTNYVVNIAALVGSDVGYVGFTGGTGGLSAVQQIQSWIFETTIAKHEVQPQLAAGGPA
                     APGDVSALTEAELALVAQEAVARWAASGLSPAQVAQLQSVQYHIGALGGGVLGLTELG
                     SSVVTLDATAGGYGWFVGPGLPGDAAFDDVVAPEEFHAGSDSPAFGRMDLLTVVAHEL
                     GHVLGLSDLDPQVAPHDLLTETLAPGVRRLPGSSIEVLSPTIVLASDALLFLSTMPQA
                     IAASDQQQPSIVTLNSPLAVNPYVEDEFARALVWAAQTSELEWLRAVPSDYNDADDAR
                     AELFAQLGDQKEFLDLFGDELES"
     gene            2344..3045
                     /locus_tag="VFB80_09080"
     CDS             2344..3045
                     /locus_tag="VFB80_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZN33959.1"
                     /translation="MVPGAASLADLEWRARESERLRTNQALSQGRAPLALINIANCGT
                     RIAEEALQHATQAQPPPPLACKQGCDWCCHLTVGTSIPEVVRIAEYLRQSLSAEELGA
                     LRERILRLDEQRRERRAAGRNEAGLPCALLRDHRCSVYPLRPLMCRGMNSSDSAECER
                     FVESSGQTPLPLYAPQLRLAAFVLDGMLAGLSNSGLAGERVELTAALRIALEVPNAAE
                     RFLAGEPVFAAARFD"
     gene            3179..3376
                     /locus_tag="VFB80_09085"
     CDS             3179..3376
                     /locus_tag="VFB80_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33960.1"
                     /translation="MRYRLRTLLIVSALGLPVLAGAWSYACAWSDADSDLLVSGLIIW
                     AIVAVCLGVWITSGIAPQRQN"
     gene            complement(3535..>5745)
                     /locus_tag="VFB80_09090"
     CDS             complement(3535..>5745)
                     /locus_tag="VFB80_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33961.1"
                     /translation="HQTYYQGSPAVEESVTAIALDYKLMSQYTSFVAVDSSKAPDESA
                     AATPPRRMLVPVPLPAGTQWEGFFGAEGDEPAEFDAFFAAPTREKKPASLQLAKQSLG
                     VPMLRWADSPARPAARYYHLPTSGPMPTGKGGGGFGYAQGFGSAVGGQSRGFQGSDTA
                     PLQQAQRRLLASDAFGRGLGFANGRAERTRGLALDGLRQLNNLGDQAKDGKRLALITD
                     GELAYTANAISPHVAKLVAATQAASEAANSKENTVTGEALRRLLTSAVLLDTAAANLG
                     HSDGSISAQAIQRLNELHQADVKAWSEKLPQLKTKLDVVLRDLSLEQAIAAVAKAANL
                     DIRLAEGCAADAAALIGESPRVSYLDLRHATVAEALDWILQAARLSWQPDAKGIVATS
                     DRRRGDSGWTYDVSAIALPLEEDLKKAGDEAKMQSEAQKAADDFLAAVRTKLRVDEAS
                     LVWFAPGHLLLFGTPAQHLALAETIATLQAGTSKPQEPLAAISEATRKRFAARQEKLA
                     RTEAVERKFDVAAIHDQFSWQLLSAAAGGKFDLEALTELQIAWQAPQTAELLTGPSRP
                     LVLRSLWTVCEAARVLPAEKELTALATSARKRSAAATNAALTEAGTNKGSLAILASAI
                     YAALANPADAAYRAKLLTLLSASETDEATKDLRLLGRVLLSEKATHSDCQGVSELVTA
                     GVGGADPVALVALAARHAGGSTWDHFRAHSRDLLGQQPLPGEVVILVNRMPGIR"
BASE COUNT          937 a         1865 c         1927 g         1016 t
ORIGIN      
        1 gaggtcggca cggccggcgc gggagcgacg acgttcatcg acaccaaccc ggatggacaa
       61 ggggtcccgg ccggcaccta ctactaccgc gtcgaggcat tcgcgccggg gttacctgac
      121 tcggccttct ccaacgtgga cagtgtccgg ttcgctgcgc ccggattgcc cctgacgatc
      181 gaccactccg aaggcttcgc cagccacggc gacctgacca cgagcgggag caccacgatc
      241 ttcccgcagc cggccccggt gggcaccttc ctgggtcacc aggatctcgg cggggtcgcg
      301 gcccccggca ctgccacttt cgacggcggg ctctacactc tgcgggcgtc gggctcggac
      361 atctgggacg tggccgactc gttccaatac gtctataagc cgctcgccgg cgacggcacc
      421 atcgaagccc gcgtggtagg cgtgcagaac accgacttct gggccaaggc cggcctcatg
      481 attcgcgaga atttgcgggc caactccaag aacgccttca tgttcgcgac cccgcccggc
      541 cacgacgaac cggtgttcca gttccgtgcg gatacgggcg gcttcagcgc cgactatggc
      601 aatcacattt ccggtctgca agcggccccg gtctggctcc ggctggtgcg gcagggcaac
      661 agcttcagcg gattctgggc ccaggacctc ggcggcggca tgcatggccc ctggaaccaa
      721 atcggccagg acaccacgat caacatggcc ggcaccgtct atgtgggttt ggcgctaacg
      781 gcccacaaca actccggtgt gctcaacacc tcgaccttcg accacgtgac catcaccggg
      841 gccacggcgc ccttgccgcc gacggtcgtg gagctgacgg atggcggctt cggcgaggcg
      901 ggcggcgcat tcttgagcaa ccgcgtggga atcgaggatt tcacgagcac cttcaccttc
      961 caaatcacgc cgggcaccag ccccatggcg gacgggttgg ccttcgtcat ccaggggctc
     1021 gggccgacgg cgctggggcc gacgggcggc gggctgggct acggctccga cttcgtcggc
     1081 ggaggcggcg gcctggggcg gagcctggcg atcaagttcg acatcttcaa caactcgggc
     1141 gagggcatca actcgacagg catcttcacc aacgggcgat cgccgaccgt ccgccagccc
     1201 ggtctcgccg ggggcttccc ggacacgtct atcaatctcg acggcacggg catcaacctg
     1261 gcgagcactc atccgttcag ggtgacgctg agctatgacg gtcgggcgct gaccgagacg
     1321 atcacggata cggtcactag cgcgacgttc acgacgaact atgttgtcaa catcgccgcg
     1381 ttggtgggca gcgacgtcgg ctacgtgggc ttcactggcg ggacgggcgg cctgagcgcc
     1441 gtgcagcaga tccagtcttg gatcttcgag accaccattg caaaacacga ggtccaaccg
     1501 cagttggcgg caggtgggcc ggctgcccca ggcgacgtgt cagcgctgac ggaggccgag
     1561 ctggcgctgg tggcgcagga ggcggtggcc cggtgggcgg cgtcgggact tagtccggcc
     1621 caggtcgcgc aacttcagtc ggtgcaatac catatcggag cgctgggagg tggcgtgctg
     1681 ggtttgacgg agttggggtc gtcggtggtg acactggacg caacggccgg cggctacggc
     1741 tggttcgttg gcccagggct gccgggcgat gccgccttcg acgatgtggt ggcgcccgag
     1801 gagtttcatg cgggctcgga cagcccggcc ttcggccgga tggatttgct gaccgtcgtg
     1861 gcgcacgagt tgggacacgt gctggggctg agtgatcttg atccacaagt agcgcctcac
     1921 gacctgttga cggaaacact ggctcccggt gtccgccggt tgccggggtc ctccatcgag
     1981 gtgctgtccc cgacaatcgt acttgcctcc gacgcattgc ttttcctatc gacaatgccg
     2041 caggcgatcg cagcgtcgga ccagcagcag ccgtcaattg tgaccctcaa ttcgccgctg
     2101 gccgtcaacc catacgtgga ggacgagttc gccagggcac tcgtttgggc cgcgcaaact
     2161 tcagagctgg aatggctccg cgcggtcccc agcgattaca acgacgcgga cgacgcacgc
     2221 gccgaactct ttgcgcagct cggggaccag aaagagtttc tcgacctgtt cggagacgag
     2281 ctggagtcct agggcaaggc ggaccagcgc cgcgttgctc ggaggtctgg gatggagcag
     2341 agcatggtgc cgggcgccgc ctcgctggcc gatctcgaat ggcgggccag ggaatctgag
     2401 cggctgcgga ccaaccaggc gctctcccag gggcgggctc cgttggcgct cataaacatc
     2461 gccaactgcg gaactcgaat cgccgaggag gctctccaac acgcgaccca ggcccagccg
     2521 ccaccgccgc tggcctgcaa gcagggatgt gactggtgtt gtcatctgac ggtggggact
     2581 agcattcccg aagtggtccg catcgcggag tacctgcgtc aatcactgtc tgcagaagaa
     2641 ctcggcgcct tgcgcgagag gatcctgcgg ctcgacgagc agcggcgaga acggagagcc
     2701 gccggccgca acgaggcagg actgccctgc gctctattga gggaccaccg ctgcagcgtc
     2761 tatcccctac ggccgctcat gtgtcgtggg atgaactcga gcgacagtgc cgaatgcgag
     2821 cgcttcgtcg aatcgtccgg ccagacgccc cttccactct atgccccgca actgcgattg
     2881 gccgccttcg ttctggatgg aatgttggct ggactgtcaa actccggcct ggcgggagag
     2941 cgggtggagt tgaccgcggc actacgcatc gccctggagg tgcccaacgc agccgagagg
     3001 ttcctggccg gcgagccggt atttgcggcc gcccggttcg attgacgctg gttacgacag
     3061 cgtcctggct gggcgacagc gcggccttca ttcttaaggc tcctgcggcc ctccaacggt
     3121 ttcatagcgc gtggaggcga tgcgaggatt atccgccgct ggcgcgtaag atccagccat
     3181 gcgctaccgc ctccgcacgc tgctgatcgt ctcggcgctg gggttgccgg tgctggcggg
     3241 ggcgtggtct tacgcctgcg cgtggagtga cgcggacagc gaccttctcg tttctgggtt
     3301 aatcatttgg gcaatcgtgg cggtctgcct cggcgtttgg atcacctcgg ggatcgcacc
     3361 gcaacggcaa aactgagggc tgcccatgtt ccggttcacg aaccgcgacg ggctgtggct
     3421 cacggtgtgg cggcgatgga ggccgcgtcg ccgtagacca ccacccgacg cccaactgct
     3481 acagtcgagg agtagccggg ccggcccgcc tttttatgcg cggatcactc ttgcctagcg
     3541 aattcctggc atgcgattga caaggataac gacctcaccg ggcaagggtt gctggcccag
     3601 gaggtcacgg gagtgggcgc ggaagtggtc ccaggttgag cctcccgcgt gtcgtgcggc
     3661 gagagcaact agggccacag ggtccgctcc gccaacacca gcggtgacca actcggacac
     3721 gccctggcag tcgctgtgag tggccttttc gctcaggagc acgcggccga gcaggcggag
     3781 gtccttcgtc gcttcgtcag tctcgctagc cgagagtaga gtcagcagct tggcccggta
     3841 agcggcatca gccgggttgg ccagcgctgc gtagatcgca ctggccagga tcgccaggct
     3901 gcccttgttg gttcccgcct cggtcagtgc cgcgttcgtg gcggccgcag accttttacg
     3961 ggcggaggtt gccaaggcgg tcagctcttt ctcggccggc aagacgcgag cagcttcaca
     4021 cacggtccac agcgagcgga gcaccagcgg acgcgacgga ccggtcagca gctcggccgt
     4081 ctgtggcgcc tgccaggcga tctgcagttc ggtcagcgct tccaggtcga atttgccgcc
     4141 ggctgctgct gagagcaatt gccaactgaa ctggtcgtgg atcgccgcca cgtcgaactt
     4201 gcgctcgacg gcttcggtcc tagccagctt ctcttggcgg gcggcgaacc gcttgcgggt
     4261 cgcttcgctg atcgctgcca atggctcctg cggcttcgac gtacccgcct gaagcgtggc
     4321 gatcgtctcg gcaagcgcga ggtgctgggc gggcgtaccg aagagcagca aatggccggg
     4381 cgcgaaccag accagcgagg cttcgtcgac cctgagcttg gtgcgaacag cggccagaaa
     4441 atcgtcggcg gccttctggg cctcggattg catcttggct tcgtcgcccg ccttcttcaa
     4501 atcctcttcg agcggcaggg cgatcgccga cacgtcgtac gtccatccgg aatcgccgcg
     4561 gcggcggtcg ctggtggcca cgatcccctt ggcatccggc tgccacgaaa gccgggccgc
     4621 ttgcaggatc caatcgagcg cctcggcaac cgtcgcgtgt cgcaggtcca gataactcac
     4681 tcgcggcgac tcgccgatca gagcagccgc atcagccgcg cagccttctg ccaggcgaat
     4741 gtcgaggttc gcagccttgg ccaccgcggc gattgcttgc tccagcgaca gatcgcggag
     4801 cacgacgtcg agcttcgtct tgagctgcgg cagcttttcg ctccaagcct tgacgtcggc
     4861 ctggtgcaat tcgttcagcc gctggatggc ctgggcggaa atgctgccat cggaatggcc
     4921 aaggttcgcc gcagccgtat cgagcagcac cgcactggtc agcaggcggc ggagtgcctc
     4981 gccggtgacc gtattctcct tgctattggc agcttccgac gctgcctgcg tggcggccac
     5041 gagcttggcg acgtgtgggc tgatggcgtt agcggtataa gccagctcgc cgtcggtaat
     5101 cagcgccagt cgcttgccgt ctttcgcctg gtcgccgaga ttgttcaatt gtcgcaagcc
     5161 atcgagtgcc aagccgcggg tgcgttcagc ccggccgttt gcaaatccca agccgcggcc
     5221 aaaggcgtcg ctggccaata gccgccgctg cgcctgctgt agcggcgcag tgtccgagcc
     5281 ctggaagccg cggctctggc cgccaacggc gctgccgaaa ccctgcgcgt aaccgaaccc
     5341 gcccccaccc ttgcccgtgg gcatcggtcc gctcgtcggc aagtgatagt agcgcgcggc
     5401 aggtcgcgcg ggcgaatcgg cccatcgcag cattggcacg cccagcgact gtttcgccag
     5461 ctgcagtgag gccggcttct tttcacgagt tggggcggcg aaaaaggcgt cgaactccgc
     5521 cggctcatcg ccctcggcac cgaagaagcc ttcccactgc gtgcccgccg gcagcggcac
     5581 ggggacgagc atccgccgcg gcggcgtggc ggcggcgctt tcgtcggggg ccttgctgct
     5641 atcgaccgcg acgaagctgg tgtactgact catcagcttg taatcgaggg cgatcgccgt
     5701 gaccgattct tcaacggcgg gcgagccctg gtaatacgtc tggtg
//
LOCUS       DASRJB010000344         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1789384, whole genome shotgun sequence.
ACCESSION   DASRJB010000344 DASRJB010000000
VERSION     DASRJB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789384"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..676)
                     /gene="rsmD"
                     /locus_tag="VFB80_09095"
     CDS             complement(113..676)
                     /gene="rsmD"
                     /locus_tag="VFB80_09095"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZN33962.1"
                     /translation="MVGLRIIGGTLRGRKLQYSGDLRTRPMKDRVREAVFNLIGPRIV
                     GAHAIDLFAGTGALGLEAISRGAARATFIERHLPTLELLKQNAAALGVAERCTANFGD
                     AFLWTGAFVPAGGTPLVVFCSPPYDFYDFRREEMLQLIGRFEQIAPSESVLVVEADAR
                     WDFGQLPQPEAWDVREYPPAFVGIYIK"
     gene            complement(1240..1848)
                     /locus_tag="VFB80_09100"
     CDS             complement(1240..1848)
                     /locus_tag="VFB80_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33963.1"
                     /translation="MGSLTRFAPLVLLAAVLAGAARDASGQSPIAGYSGPPLEPPAQL
                     MLIRNALALLNHGNLTGNYTVLRDLASPRFRERNSAADLSGTFANLRQKNLDLSPILV
                     IQPQLTQAPQIVGDRLQMVGLFPTRPQAVQFALAFQQVEGGWMIDEISLRVAPADLQP
                     PGAAPPNAAAERRPVTTYAPPAGDIRTAERGPPLPVPGSRPR"
     gene            complement(1866..3134)
                     /locus_tag="VFB80_09105"
     CDS             complement(1866..3134)
                     /locus_tag="VFB80_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HZN33964.1"
                     /translation="MIQTRNCLSGNCRAERLTALLLAWLLWPAGHASGQFLGTNFVTG
                     DADGAVASTASSAAAGTNAAASGEFSTAVGSSATASGIASTAVGQSSAATANATTALG
                     QASNASGFASTALGFSSAASAESATAVGVFAQASGLNSTALGRTAAATGFYSTALGSQ
                     ATASGDAATAVGVNAVASASASTSLGTGATATSDGTTALGHGAIASGFVGTAVGASAV
                     ASGHSSVALGTFANGNNLGSIAIGRSSKALADEAIAIGHSANAAFANSIAIGHGVATT
                     RDNQIALGTVDNTYTAAGISSAASKAAQSGPVELVTTDASGNLASAAAGDLGLASAGD
                     LQMLRSDLDRTTSGVAMAMALSGVPNILPEYTNYAVSLGWGGFGGASALAVGGSARVT
                     DNLFFNAGGAFGAGGRGSGGGGRAGFTYAW"
BASE COUNT          585 a         1082 c         1076 g          567 t
ORIGIN      
        1 gggggcaaac tttccgcccg cgggtcgagg ccgaccagga cggggttgcc ctgctggcgg
       61 acggccgcgg cgagccggtc gacaaagtgg gtggtcgctt ggttcgtcat attcacttga
      121 tgtaaatccc cacgaaggcc ggcgggtatt ctcgcacgtc ccacgcctct ggctgcggga
      181 gctgcccgaa atcccagcgg gcgtcggcct cgacgaccag cacgctctcg ctcggagcga
      241 tctgttcgaa gcggccaatg agttggagca tctcttcgcg gcggaaatcg tagaagtcgt
      301 aaggcggcga gcagaaaacg acgagcggag tcccgccggc aggcacaaag gccccggtcc
      361 agaggaacgc gtcgccgaaa ttcgccgtgc accgctcggc cactcccagc gcagccgcgt
      421 tctgcttgag caattccagc gtcggcaaat gccgctcgat gaaggtcgcc cgcgcggccc
      481 cgcggctgat cgcctccaga ccgagcgcgc cggtgccggc gaagaggtcg atcgcatgcg
      541 cgccgacgat ccgcggaccg atcaggttga agactgcctc ccggacgcgg tctttcatgg
      601 gccgcgtccg caagtcgccg ctgtactgga gcttgcgccc ccgcaacgtg ccgccgatga
      661 tccgcagccc gaccacgggc gtctcagcgc ctcgcccggc ctcgtttgac ctttgaatcc
      721 ttcgcattcc ggcagtttag cggcaaagcg cgacgtccgg acggcggccc gcccgctggt
      781 cgtttccgtc ggccatgcgc gcatttcgcc aaatctttcc cgagcatcaa cttgagtcgt
      841 aactgcagtc gcgacaatgc cttacggcga aatccacgcg attccaatta tgcgcattat
      901 gcgcgccgcc ggctgggaca tcccccgcgg cgccagacat gcatgcataa tcgccgcact
      961 ttccaacgga gcatttcaac gcaagtaact gcgttgacac gacttacgac gtaaatgccg
     1021 tatgcacgtt atgcagaata tgcagcttag tggtcagacc tgcagagcac tccaaacaac
     1081 cgcggccgcg accggggatt ccaaacaacc aaattgccaa agccgcccgc cacccgcatc
     1141 gggcggcagc agtagcaggg aattgtatct attgtccact atttgtcaag aagagggcag
     1201 ccgtgattct cgctgttgaa aatgcgcgcc cacaacgtct caccgtgggc gcgatcctgg
     1261 cacgggtagc ggcgggccgc gctcggccgt gcggatatcg cctgccggcg gcgcgtaagt
     1321 tgtcaccggt cgccgctcgg ctgcagcatt gggtggcgcg gcccctgggg gctgcaggtc
     1381 cgcgggagcc acgcgcaacg agatctcgtc gatcatccag cccccttcga cctgctggaa
     1441 ggcgagcgcg aattgcactg cctgcgggcg cgtgggaaag aggccaacca tttgcagccg
     1501 gtcgcctacg atctggggcg cttgggttaa ttgcggctgg atgacgagaa ttgggctcaa
     1561 gtccaggttt ttctgacgca ggttggcaaa ggtcccggac aggtcggccg ccgaattccg
     1621 ctcgcgaaag cgaggcgagg ccaggtccct gagcacggtg taattgccag tcagattgcc
     1681 gtgattcagg agggcgagcg cattgcggat gagcatcagc tgggccggcg gctcgagtgg
     1741 cggacccgaa tagccggcga tgggggactg gccagatgcg tcgcgcgcag cgccggcaag
     1801 cacggcagcc aggagcacga gcggagcgaa tcgcgtcagc gaacccatgt gctattcctg
     1861 cgaatttacc aggcgtaggt gaatccggcg cggcctccgc cgccgctgcc gcggccgccc
     1921 gcgccaaagg cgccgcccgc attgaagaac aagttgtcgg taactcgcgc cgacccgccc
     1981 acagcgaggg cgctggcgcc gccgaacccg ccccagccca gcgagacggc gtagttcgtg
     2041 tattcgggca agatattcgg tacgcccgaa agggccatgg ccatggcgac ccccgacgtg
     2101 gttcgatcga ggtcgctgcg caacatctgc aggtcgccgg cgctcgccag tcccaaatcg
     2161 cccgctgccg ccgacgccag gttgccgctc gcgtcggtcg ttaccagctc gaccggcccg
     2221 gactgtgccg ctttgctcgc ggccgaactg atgccagcgg ctgtataggt attgtccacc
     2281 gtacccagcg cgatttgatt gtcgcgggtc gtcgctacgc catgaccgat ggcgatggag
     2341 ttggcgaaag ctgcgtttgc gctgtggccg atggcgatcg cttcgtcggc tagcgctttt
     2401 gaagacctgc cgattgcgat cgatcccagg ttgtttccat tcgcgaaggt gccaagggcg
     2461 accgacgagt ggccactggc gaccgcggat gctcccacgg ccgtgccgac gaatccgctc
     2521 gcgatcgcac cgtggcccag cgcggttgtg ccgtccgacg tggcggtcgc gccggttccg
     2581 agcgacgtgg aggcgctggc actggccact gcgttcacgc cgacggccgt ggcggcatcg
     2641 ccggaggccg tggcctgcga tcccagggcc gtcgaataga agccggttgc ggcggccgtg
     2701 cggccgaggg ccgtcgaatt cagcccgctc gcctgcgcga atacccctac cgcggtggcg
     2761 gactctgcgc tggccgcgga actgaaaccc agggccgtcg aggcaaatcc gctggcgttc
     2821 gacgcctggc ccagggcggt cgtcgcattg gcggtcgcgg cggagctttg gccgactgca
     2881 gtcgaggcaa ttccgctcgc cgtggcagac gagcccactg ccgtggaaaa ctcgccgctg
     2941 gccgcggcgt tggttccagc ggcggccgag ctagcggtcg aggccaccgc cccgtcggcg
     3001 tcgcccgtta cgaaattcgt ccccaaaaac tggccgctgg cgtgtccagc cggccacaac
     3061 aaccaggcga gcaatagtgc cgtcagtctc tcagcacggc agttgcccga taggcaattt
     3121 cgcgtctgaa tcatcgtgat cgcctccatt ccgcggctgc tttcgcataa ctggcgcggc
     3181 agcgtgcggt cggtgcccaa ccgcgagccc tgccgaattt gaccttgact accgataagt
     3241 tgttagtaga taacactcgt caatagtgcc gggcgaggat cagtcaaagt gaatgacgaa
     3301 accttaaatc
//
LOCUS       DASRJB010000345         9823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1223946, whole genome shotgun sequence.
ACCESSION   DASRJB010000345 DASRJB010000000
VERSION     DASRJB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223946"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..828)
                     /locus_tag="VFB80_09110"
     CDS             complement(253..828)
                     /locus_tag="VFB80_09110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN33965.1"
                     /translation="MWLVSTAAVGGNLPFRLEAGEYLVGRTKASAIVIRHATVSRRHA
                     RFQHTRGKLILTDLDSCNGTYVNDRAVARAELQIGDRLRFGGVPCLLAQSPLTGNSVE
                     DTEPTYRLSEPAATTRIDGLTAVQSIVATYLLEGHSEAEIAARMNKSRHTIHTHMKAI
                     FARLGVHTRADLILQLLKPSDRGPLPRERIP"
     gene            1066..3423
                     /locus_tag="VFB80_09115"
     CDS             1066..3423
                     /locus_tag="VFB80_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN33966.1"
                     /translation="MPQPANSPSAGRHYRRLLQARERPPIALEGAGGGLESLEVATDA
                     DLSDAAIKDRLSQAGSSLSQFVRKELGGDPRLQSIVDEIVRQGDAALRALRDDESQLD
                     RNPQLMAGLEAIARTDGSRPSFLIRDGQVDPASSPIGAWADRLAEREESLRQAIACVG
                     RVNLGIEHRGTGFLIQENLIVTNRHVLQEIADPSGNGTWTFRPPGAWIDFGHEHRGRA
                     SVNRRALARVIFAGKLPISRFSMDHGRLDLALIELGPGDPAAAPQRVLSVLAMPAWDQ
                     PQTAIFAVGYPGAPPRHAYPATLLEQLFQSTFGHKRLAPGELLPRESTYSDWTLGHDA
                     TTLGGNSGSALLVVGNEMVAAGLHYGGLPQVGPQPGTNWGHVLGLVLGQTDGVRSQTL
                     GEILRQRGVEVLDGGNSAPREAARTPSPATPMKERMPAEPAPSPVPALSVSAAPSVAK
                     VTASAASGVYSWTVPLQVTISLGQPTPSARITGAPSKEGLFGPSVPLAEVAARFSADS
                     LAATQFSWETALSLALASKLAYSPAALVHSTGLSDWRLATCAFFEESDTQCFVASTPA
                     AAFVAFRGTESLGDWLADLNVASIRRPYGVVHRGFYFAYQVIQAQLEQALAAVGSRPL
                     LLTGHSLGAALATIAAAELHDRWRIAGIYTYGQPRVGFSSFVSFFNQRFGTKLYRFVN
                     DDDIVPRIPPGYQHVGRLFHFTAQGSLESTLESAAPGLTATELPPLTDAEFDQLRSEL
                     LATRASRKAGARESLLPTPALEGFFPSISDHSMDEYIPKIARQIR"
     gene            complement(3430..7428)
                     /locus_tag="VFB80_09120"
     CDS             complement(3430..7428)
                     /locus_tag="VFB80_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6797 domain-containing protein"
                     /protein_id="HZN33967.1"
                     /translation="MNRSCLCCLVLAVDALVVALPRSAICQEQPPAASRLFARDNLVA
                     WCIVPFDARRRGPEERATMLKRLGFSKFAYDYRAEHIPTFDAEIEALKKQGIELTAWW
                     FPQTLNAEAKQILAVLERHKIKTQLWITGGGGPAANDDERRQRIAAEAARIRPIAEAA
                     DKIGCTVALYNHGGWFGEPENQLAVLEELKLPNVGLVYNLHHGHAHVGRLAEVLPKML
                     PHLVCLNLNGMDADGEATGRKILPLGQGELDLALLKTIVASGYRGPIGILGHTQDDAE
                     ARLQDNLDGLAWLVPQLDGKPAGQRPTPRTPVPARPKPAAGAHGAHHAAAPAAAIAAD
                     YDPQAAGELIAAALQHGDARRGMAVFRAARFACQSCHQVGPHGGSVGPALSDAGKRLK
                     PEEIAEAVLWPRRTVKPEFVAWKLLLADGRTIQGYKRSESPDSIELFDPAAQRTDSIA
                     KRDIEQQLEAGTLMPDGLAAAMSPAQRRDLVRLLIDLGRTPGLEQEVGPAETPAAFSY
                     DRQPLDTGAWKLWQHPVNRDRLYDFYLKEALFFRSQRGGPHLLPAFPGMDGGKLGHWG
                     NQNEETWKDARWNQTNLGSLLCGVFRAPGVEVPKGVCVRLGERGEMACCFNPQTLEYE
                     AVWKGGFLKFSEIRHGFLDGLRPAGEMLPPPVVVRPGKPFFYRGFYRLGSRIAFAYRL
                     GDEEWLDAPWVKDGQFERVVGRANEHPLRAALKGGPPQWPQELPTKGELGSAQTYAVD
                     TIQPPFENPWKALLFFSGHDFLPDGTAFLATMTGDVWRVSGLDADLQNVRWRRFASGL
                     HQPLGLVVAEGQIYVLGRDQITRLVDLNGDGEADFYECVSNRMDTSPAGHDFICGLAR
                     DREGRFITASGKQGLIRISADRQQVETLATGFRNPDGLGLLPDGCVTVPCSEGEWTPA
                     SMICLVKPVERASGRPPHFGYQGPQDGQPPALPMVYLPRGLDNSSGGQAVAADERFGP
                     LAGQIIHFSSGQGCQFLILRDEVAGQPQGAVVPLAGEFRSGAHRGRVNPADGQLYVSG
                     MAGWGTYTPDDGCFHRVRYTGGRGQFPKSLHIHENGVLLSFTEPIDRAALADAGKQFA
                     QVWNYRYSSGYGSPELAPSHPGVVGHERLEIAGVHPIDERTLFVELPDLQPVNQLHLV
                     LQVDSGRPQELFVTVHKLDKPFAQIPGYAPSEKLIAAHPQSVDLALLGKTIPNPWGKR
                     GRAVPTATLKVAAGKNLTYSTRLLRAKAGERVQLTFTNPDVVPHNWVLVKPGTLAAVG
                     DLANKLVADPEAVLRQYVPQTADVIAYTDIVPPQQSHEIWFDAPREKGRYPYLCTFPG
                     HWMVMNGELVVE"
     gene            7577..>9823
                     /locus_tag="VFB80_09125"
     CDS             7577..>9823
                     /locus_tag="VFB80_09125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN33968.1"
                     /translation="MKRTFWQAACCTVLLLGATHIRAADFAVLSPATWDAFAPQGKEV
                     DAIYGDYVLRNERLIAVIAQPLATRNANMTVRGVGGCLIDFTERTSQNDQLSAYYPAA
                     GRYPLTSPANVRLLADGKPLPELPAAPVAAKTLALELDAEPVEDRPRLTLRYTLAEGA
                     SELLVETIYSNPTDKAVSDDLSDSIRADRTFTFGFDPATSCFLAADEWFRQAYGVVAA
                     GYEIKGTGQRGTLLQLVKDGTSKLTLEPGASHTIARKMFVGTSGLAARGQSSQLAGQK
                     VGRMTVRLIDVEPARQARVELKHEGVVYGGGRTDAEGKIDFLLAAGRFTAEVKTLDGR
                     GGTLQLEGGADRTVDLPLAPASYALARITDDRGRPIPAKVSFAGKGGTADPDWGPDSG
                     EIAVKNVYYTHNGSFKQAIAPGKYDVIVSYGPEHDAVFTTLEVPPAATAALNAVLKRS
                     VDSAGWVSADFHSHSSPSGDNTSSQLGRVLNLLCEHIEFAPCTEHNRIDTYTPHLKRL
                     GIERLMATCTGMELTGSPLPINHQNAFPLLHKPRTQDGGAPVTDVNPIVQIERLALWD
                     NKSEKLVQMNHPNLPQILGDRDLDGKPDAGFERMFGFVDVIEIHPPQGIFTPPQKQAD
                     GKLERNPTCHWMQMLNLGYRHPGVINTDSHYNYHESGFFRNFIHSSTDDPARIGVMEM
                     VHNAEAGHVVVSTGPFLTVDAIGDSLGKEVGAMPGMDLPATGGRVTLHVRVQCPNWFD
                     INRVQVFAN"
BASE COUNT         1611 a         3311 c         3265 g         1636 t
ORIGIN      
        1 tcggtggccc gcgcctgctg ggccggcgaa ggcgacgaag gagcaaccga ctccaggccg
       61 ccggccggcg ccgtggtggt ttgagcggga cgggcaaagg ggacttccgg agcgagattc
      121 atggcgtact ccaggatgga aggggttgac aaggccgcca cgcgaatcag ccgcttccat
      181 gcacgcctgc gacccgcgcg cgagcgcggc gcggcgattc tcgcccgccg gccgcggatg
      241 acaataccct cgctagggta tccgctcgcg cggcagcggt ccgcggtccg agggcttgag
      301 cagttgcaag atcaaatccg cgcgtgtgtg aacgcctagg cgggcgaaga tcgccttcat
      361 gtgcgtatga atcgtgtgtc ggctcttatt catcctcgcg gcgatctccg cttcgctatg
      421 gccttccagc aggtaagtgg ccacgatcga ttgcactgcg gtcaagccgt cgatgcgggt
      481 ggtcgccgcg ggctcggaga ggcgataggt aggttccgta tcttccacgc tgttgccggt
      541 tagcggcgat tgggccagca ggcacggcac gcctccgaac cgcaggcggt ccccgatctg
      601 aagttcggcg cgggccacgg cccggtcgtt cacgtacgtg ccgttgcagc tgtccaaatc
      661 ggtcaggatc agcttgccgc gggtgtgctg aaaccgggcg tgccgccgcg acaccgtggc
      721 atggcggatg acgatggccg aagccttggt gcgcccgacc agatactcgc ctgcttcgag
      781 tcggaacggc aagttgccgc cgacggctgc tgtgctcacc agccacatgg cacccctcgc
      841 attcaactcc cggtcgcgga tttgcgcgga cggccacacc ctggttagtc gtaattcagc
      901 ccggcgcgcg ggtctaaaaa agaatttgga gttttccttg cgccggcccc gtacccagat
      961 tagggtatgg catctggtcc ggtggccgca acaatcacgt ccggcacgtg tccgtccgcc
     1021 ccgagggttt ctcatctcct ctgctccttt aaaggagtcg ctacgttgcc tcaaccagcc
     1081 aattcgccct ccgctggccg ccactaccgc cgcctcctgc aagcgcgtga gcggccgccc
     1141 atcgcgctgg aaggcgccgg cggtgggctg gagtcgctcg aggtcgccac cgacgccgac
     1201 ttgagcgatg ccgccatcaa ggaccggctg agccaggctg gctccagctt gtcgcagttc
     1261 gtgcgcaagg aattgggggg cgatccgcgg ctccagtcga tcgtcgatga aatcgtacgc
     1321 caaggggatg cggccctgcg agcgctccgc gatgacgagt cgcagctgga ccgcaatccg
     1381 cagctcatgg ccgggctgga ggccatcgct cgcaccgatg gttcgcgccc ttcgttcctg
     1441 attcgcgacg gccaggtcga tcccgcgtcg agcccgatcg gggcctgggc cgaccggctc
     1501 gcggagcgcg aggaatcgct ccgtcaggcg atcgcctgcg tggggcgggt gaacctggga
     1561 atcgagcacc gcggcaccgg ctttttgatt caagagaacc tgatcgtgac caatcggcac
     1621 gtcctgcagg agattgccga cccgtccggc aatggcacct ggacgttcag gccgcccggc
     1681 gcgtggatcg acttcggcca cgagcaccgg ggccgcgctt cggtcaatcg ccgcgcacta
     1741 gcgcgggtga tctttgcggg taagctgccg atcagccgct ttagcatgga ccacggcagg
     1801 ctcgacttgg cgctgatcga gctggggccg ggcgaccccg ccgccgcgcc gcagcgcgtg
     1861 ctgtcggttt tggccatgcc ggcctgggac cagccgcaga cagcgatctt cgccgtcggc
     1921 tatcccggcg cgccgccgcg gcacgcctat ccggccacgc tgctggagca actgtttcaa
     1981 tccacgttcg gccacaagcg cctggcgccc ggtgaattgc tgccacggga atcgacctac
     2041 agcgattgga cgctgggcca cgacgccacg acgctgggag gcaactccgg gtcggcgctg
     2101 ctggtcgtgg gaaacgagat ggtcgcggcg ggcctccatt acggtggcct gccgcaagtc
     2161 ggcccgcagc cggggacaaa ctggggccac gtcctgggac tggtgctggg acaaaccgac
     2221 ggcgtccgca gtcaaacgct gggcgaaatt ctgcggcagc gcggcgtcga ggtgctcgat
     2281 ggcgggaatt ccgcgccgcg ggaagcagcc cgaacgccca gccccgctac accaatgaag
     2341 gagcggatgc ctgctgagcc agcgccgtcc cccgtgcccg cgctgtccgt aagcgccgcg
     2401 ccttctgtgg ccaaggtgac cgcgtccgcg gcttccggcg tctacagctg gacggtcccg
     2461 ctgcaagtga cgatcagcct gggccagccg acgccgtctg ccaggattac cggcgctccg
     2521 tctaaggaag gccttttcgg tccgtccgtg ccgctggccg aagtggccgc gcgattttcc
     2581 gccgactcgc tggcagcgac gcaattcagc tgggaaacag ccctctcgct ggcactggcc
     2641 agcaaactcg cctattcgcc ggcagccttg gtccacagca cggggctgag cgactggcgg
     2701 ctggcgacct gcgcgttttt tgaggagtcc gacacgcagt gctttgtggc ttcaacgccc
     2761 gcggctgctt tcgtcgcgtt tcgcggcacg gaaagcctgg gcgattggct ggccgacctg
     2821 aacgtggcga gcatccgccg cccctacggc gtggtccatc gcggcttcta tttcgcctac
     2881 caggttatcc aggcgcagct cgagcaggcg ctggccgcgg tcggctcccg gccgttgctt
     2941 ttgacgggcc acagcctcgg cgcggccctg gcgacgatcg ccgccgccga gcttcacgat
     3001 cgctggcgaa tcgccggcat ctacacctac gggcagccgc gcgttggctt cagcagcttc
     3061 gtctcgtttt tcaatcaacg ttttggcaca aagctgtacc gatttgtcaa cgacgacgat
     3121 atcgtgccgc ggattccgcc cggttatcag cacgtcgggc ggctgtttca cttcaccgcc
     3181 cagggcagcc tcgaaagcac cctcgagtcc gcagcccccg gccttaccgc caccgagctg
     3241 ccgcccctca ccgacgccga gttcgaccag ttgcggtccg aattgctcgc cacccgcgct
     3301 agccgcaaag ccggcgcccg cgaatcgctc cttcccacgc ccgcgctgga aggcttcttc
     3361 cccagcatca gcgaccacag catggacgag tacatcccca agattgcccg gcagattcgg
     3421 taggtcgcat cactcgacga ccagctcgcc gttcatcacc atccagtggc cggggaacgt
     3481 gcagaggtat ggatagcggc ctttctcgcg cggggcgtcg aaccagattt cgtgcgactg
     3541 ctggggcggg acgatgtcgg tataggcgat cacgtcggcg gtttgcggca cgtactgccg
     3601 cagcacggcc tcgggatcgg cgacgagctt gttcgccaga tcgccgacgg ccgccagcgt
     3661 gcccggcttc acgagcaccc agttgtgcgg cacgacgtcg gggttggtga aagtcagctg
     3721 cacccgctcg ccggctttgg cccgcaagag ccgcgtcgaa taggtcaggt tcttcccggc
     3781 ggcgactttc agcgtggccg tcggcacggc gcggccgcgc ttgccccagg gattgggaat
     3841 cgtcttgccc agcagtgcca gatcgaccga ctgcggatgg gcggcgatga gcttctcgct
     3901 cggcgcatag ccggggattt gcgcgaatgg cttgtcgagc ttgtggaccg ttacgaacaa
     3961 ctcctgcggc cggccgctat cgacttgcag caccaggtgc aactgattca ccggctgcaa
     4021 atcgggcagc tcgacgaaca aagtccgctc gtcgatcgga tggacgccgg caatctccag
     4081 ccgctcgtgg ccgacgacgc ccggatggct cggcgcgagc tccggcgagc cgtagcccga
     4141 gctgtagcgg tagttccaga cctgggcgaa ttgctttccc gcgtcggcca gggcggcgcg
     4201 gtcgatcggt tcggtgaacg acagcagcac gccgttttcg tggatgtgca gcgacttggg
     4261 aaactgcccg cggccgccgg tgtatcgcac tcggtggaag cagccgtcat cgggcgtgta
     4321 ggtgccccag ccggccatgc ccgagacgta gagctggcca tcggcgggat tcacccggcc
     4381 gcggtgcgcc cccgagcgga attcgccggc tagcggcaca actgctcctt gcggctgccc
     4441 ggcgacctca tcgcgcagga tgagaaactg gcatccctgg cccgacgaga aatgaatgat
     4501 ctggccagcg agcgggccaa aacgctcgtc ggcggcgacc gcctggccgc cgctgctgtt
     4561 gtccaggccg cggggcagat agaccatcgg cagcgcgggc ggctgaccgt cctgcgggcc
     4621 ttgataaccg aaatgcggcg gccggccaga ggcccgctcc acgggcttga ccaggcagat
     4681 catcgaggcg ggcgtccatt ccccttcgct gcaggggacg gtgacgcagc cgtcgggcaa
     4741 gaggccgaga ccgtcggggt tgcggaaacc ggtggcgagc gtctcgactt gttggcggtc
     4801 ggcggaaatg cggatcaaac cttgcttgcc gctggcggta atgaatcgcc cttcgcggtc
     4861 gcgggccagg ccgcagatga aatcgtggcc ggcgggcgac gtatccattc ggttcgagac
     4921 gcactcgtag aaatcggctt cgccgtcgcc gttcaggtca actagccgcg tgatctggtc
     4981 gcggccgagc acgtagatct ggccctcggc gaccaccagc cccagcggct gatgcagtcc
     5041 gctggcaaac cgccgccagc gaacgttctg caggtccgcg tcgaggccgc tgacgcgcca
     5101 cacgtcgccg gtcatcgtgg cgaggaaggc cgtgccgtcc ggaagaaagt cgtggccgct
     5161 gaaaaagagc agcgccttcc agggattctc gaacggcggc tgaatcgtat cgacggcata
     5221 ggtctgcgca cttcccagtt cgcccttggt cggcaactct tgcggccact gcggtggccc
     5281 gcccttcagt gccgcccgta gcggatgttc gttggcgcgc ccgacgacac gctcaaactg
     5341 cccgtccttc acccacggcg cgtcgagcca ctcctcgtcg ccgaggcggt atgcgaaggc
     5401 gatccgcgag cccagccggt aaaagccgcg atagaagaat ggtttcccag gccggacgac
     5461 aacaggcggc ggcagcattt cgccggccgg ccgcaggccg tcgagaaagc cgtggcgaat
     5521 ctcagagaat ttcaggaagc cgcccttcca gacggcttcg tattccagcg tctggggatt
     5581 gaagcagcag gccatttcgc cgcgctcgcc caaccggacg caaacgccct tgggcacttc
     5641 gacgcccggc gctcgaaaga cgccgcacag caggctgccc aggttggttt gattccagcg
     5701 agcgtccttc cacgtctctt cgttctggtt gccccagtgg ccgagcttgc cgccgtccat
     5761 gccgggaaat gccggcagca gatgcggacc gccccgttgc gagcggaaga agagcgcctc
     5821 cttgaggtag aagtcgtaca ggcggtcgcg gttgaccgga tgttgccaga gcttccaggc
     5881 gcccgtgtcg agcggctggc gatcgtagct gaaggcggcc ggagtctcgg cggggccgac
     5941 ttcctgctcc aggcccggcg tgcggcccag gtcgatcaag agccgcacca ggtcgcggcg
     6001 ctgggccggc gacatcgcgg ctgccaggcc gtcgggcatc agcgtgccgg cttcaagctg
     6061 ttgctcgatg tcgcgcttgg cgatcgaatc ggtccgctgc gccgctgggt cgaagagctc
     6121 gatcgaatcg ggcgattcgc tgcgcttgta gccctggatc gtgcgcccgt cagccagcag
     6181 cagcttccag gcgacgaatt ccggcttcac cgtccgccgc ggccaaagga cggcctcagc
     6241 gatttcctcg ggcttcagcc gcttgccggc atccgatagc gccgggccga ctgagccgcc
     6301 atgcggaccg acctggtggc acgactggca ggcaaaccgc gccgcgcgaa acacggccat
     6361 cccgcggcgg gcgtcgccgt gctgcagggc ggccgcaatc agctcgcccg ccgcctgtgg
     6421 atcgtagtcg gcggcgatcg cggcggccgg cgcggcggcg tggtgagcgc cgtgcgctcc
     6481 agccgccggc ttgggccgcg cgggcaccgg cgtgcgcggc gtcggccgct ggccagcggg
     6541 cttgccgtcg agctgtggca ccagccaggc caggccatcg aggttgtcct gcaggcgagc
     6601 ttcggcgtcg tcctgcgtgt ggccgaggat gccgatcggg ccgcgatagc cgctggcgac
     6661 gattgtcttg agcagcgcga ggtcgagctc tccctggccc agcggcagaa tcttgcggcc
     6721 ggtcgcttcg ccgtccgcgt ccatcccgtt gaggttcagg cagaccaggt gcggcagcat
     6781 cttgggcagc acctccgcca ggcggccgac gtgcgcgtgc ccgtggtgca ggttgtagac
     6841 gaggccgaca ttcggcagct tcagctcctc caacaccgcc agctgattct ccggctcacc
     6901 gaaccagccg ccatggttgt agagcgcgac cgtgcagccg atcttgtccg ccgcctcggc
     6961 aatcggccgg atgcgcgccg cttcggctgc aattcgctgg cggcgctcgt cgtcattggc
     7021 cgccggcccg ccgccgccgg taatccagag ctgcgtcttg atcttgtgcc gctccaggac
     7081 ggcaagaatc tgcttcgcct cggcgttcag cgtttgcggg aaccaccagg ccgtcagctc
     7141 gatgccttgc ttcttcagcg cctcgatttc ggcgtcgaac gtcgggatgt gctcggcgcg
     7201 gtagtcgtag gcgaatttcg agaagcccag ccgcttcagc atcgtggccc gttcctctgg
     7261 cccgcggcgc cgcgcgtcga acggcacgat gcaccaggca accaggttgt cgcgggcgaa
     7321 gagccggctg gcagccggcg gctgctcctg acatatcgct gagcgcggca gagcaaccac
     7381 cagcgcgtcc acggccagga ccaaacaaca caagcaggag cgattcatgg cggcctcttt
     7441 tcgctggagc ctacttttgc cgcgcagcgg aagcaatgga agagccggta gcggagaggc
     7501 aggccaacgg ttgaattgcc tcgcgtgctg cgcaatactg cggccggctg gtattcacac
     7561 gctggttgaa ttacctatga aacgcacatt ttggcaggcg gcctgctgca cggttttgct
     7621 tctaggcgcc acgcacatac gcgcggccga tttcgctgtc ctatcgcccg cgacctggga
     7681 cgccttcgcc ccgcagggca aagaggtgga cgcgatctac ggcgactacg tgctccgtaa
     7741 cgagcggctg atcgccgtca tcgcccagcc tctggccacc cgcaacgcca acatgacggt
     7801 ccgcggcgtc ggcggctgcc tgattgactt taccgagcga acgagccaga acgaccagct
     7861 cagcgcgtat tatccggccg cggggcgcta tccgctgact tctccggcca atgtgcggct
     7921 gctggccgac ggcaagccgc tccccgagct tcctgccgcg ccggtggccg ccaagacgct
     7981 ggccctcgaa ctcgatgcgg agcccgtcga agaccggccg cgcttgacgc tccgatacac
     8041 gctggcggaa ggggccagcg agctgctcgt cgagacgatc tacagcaatc cgactgacaa
     8101 ggcggtcagc gacgacctga gcgactcgat ccgggccgac cgcacgttca cgttcggctt
     8161 cgatccggcc acgagctgct tcctggctgc cgacgagtgg ttccgccagg cgtatggcgt
     8221 cgtcgccgcc ggctacgaga tcaaaggcac gggccagcgc gggacgctgc tgcaactggt
     8281 gaaggacggc acgagcaagc tgacgctcga gcccggagcg tcgcacacga tcgcccgaaa
     8341 aatgttcgta ggaaccagcg ggctggccgc gcgcgggcag tccagccagc tcgcgggcca
     8401 gaaggtcgga cggatgacgg tccgtctcat cgacgtcgaa cccgcgcggc aggcccgcgt
     8461 cgagctgaag cacgagggag tcgtgtatgg cggcggccgc accgacgccg agggaaagat
     8521 cgacttcctg ctggcggcgg ggcggttcac ggcggaagtg aaaacgctcg acggccgcgg
     8581 cgggacgctc cagctcgaag gcggcgcgga ccgcaccgtc gatctgccgc tcgcgccggc
     8641 gagttatgcc ctcgcgcgga tcaccgacga ccgcggtcgc ccgattcccg ccaaggtctc
     8701 gttcgcgggc aagggcggca cagccgatcc cgattggggg cccgactcgg gcgagatcgc
     8761 cgtcaagaac gtctattaca cgcacaacgg ctcgttcaag caggcgatcg cgccgggcaa
     8821 gtacgatgtg atcgtcagct acggccccga gcacgacgcg gtgtttacaa cgctcgaagt
     8881 tccgccggcc gccaccgcgg ccttgaatgc cgtgctgaaa cgctcggtcg attccgccgg
     8941 ctgggtcagc gccgactttc acagccattc cagcccctcg ggcgacaaca cgtcgagcca
     9001 gctcggccgc gtcctcaatc tgctctgcga gcacatcgag ttcgctccct gcaccgagca
     9061 caaccgcatc gacacctaca cgccgcacct gaagcggctg gggatcgagc ggctgatggc
     9121 cacctgcacc ggcatggagc tgacgggttc gccgctgccg atcaatcacc agaatgcgtt
     9181 cccgctgctg cacaagccgc gaacgcagga cggcggcgcc ccggtgaccg acgtcaatcc
     9241 gatcgtgcag atcgagcggc tggcgctgtg ggacaacaag agcgagaagc tggtgcaaat
     9301 gaaccatccg aacctgccgc agatcctggg cgaccgcgac ctggacggca aacccgacgc
     9361 cggcttcgag cggatgttcg gctttgtcga tgtgatcgag attcatccgc cgcaaggcat
     9421 cttcacgccg ccccagaaac aggccgacgg caagctggag cgcaatccga cctgtcactg
     9481 gatgcagatg ctaaacctgg gctaccgcca cccgggcgtg atcaacaccg actcgcacta
     9541 caactatcac gagtcgggct tcttccggaa tttcattcac agttcgaccg acgatccggc
     9601 ccggatcggc gtgatggaga tggtgcacaa cgccgaagcc gggcacgtcg ttgtcagcac
     9661 cgggccgttt ttgacggtcg atgccatcgg cgactcgctg ggcaaggaag tcggcgcgat
     9721 gccggggatg gatctgcccg cgaccggcgg ccgcgtgacg ctgcacgtcc gcgtgcagtg
     9781 ccccaactgg ttcgacatca accgcgtgca ggtctttgcc aac
//
LOCUS       DASRJB010000346         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4046742, whole genome shotgun sequence.
ACCESSION   DASRJB010000346 DASRJB010000000
VERSION     DASRJB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4046742"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1481
                     /gene="purB"
                     /locus_tag="VFB80_09130"
     CDS             54..1481
                     /gene="purB"
                     /locus_tag="VFB80_09130"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZN33969.1"
                     /translation="MPHDRYDNPLSSRYASPEMSALWSEQRKFSTWRRLWVILAEAER
                     ELGLPINDQQIAELRAHTDDIDFQAAAAYEKKLRHDVMAHVHAYGDVCPAARPIIHLG
                     ATSCYVTDNADLLLMREGLQLLARRTAAVIDQLARFAAEHRDLPCLGFTHLQPAQPTT
                     VGKRACLWAYDLALDLAELEHRIASLKSRSIKGTTGTQASFLALFGGDHAKVRRLERL
                     VAQKIGFESSYAVTGQTYSRKVDSQVLDALSGLGQSAHKLATDLRLLASRKEMEEPFE
                     ADQIGSSAMAYKRNPMRSERVCGLARFLMSMQSNAAATAAVQWMERTLDDSANRRLVI
                     PQAFLAADAILLLLQNITSGLVVYPRSIAANLAAELPFMATENILMAAVSAGGDRQDL
                     HERIRRHSQAAALRVKQEGLPNDLLERLRHDPAFAKVDLASVTEARQFVGRAPQQVDE
                     FLADVVAPIRARYPAAAGPPAEISV"
     gene            1492..2556
                     /locus_tag="VFB80_09135"
     CDS             1492..2556
                     /locus_tag="VFB80_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN33970.1"
                     /translation="MKTSLKIAVYAGDGIGPEVIDEALNVLAAVQALDGSFQLEFTRL
                     NWGALHYRQHQTVVPDDFLDVLRPLDAILLGAVGWPALIPDHITLAPLVKIRQSFDQY
                     ACVRPSKLYPGVKSVLAGKGPADIDFVVIRENSEGEYVDNGGRLKRGTPDEVAVQSAL
                     HSRKGVERILRYGFDLARRRRSRLTMATKSNAQRHAYVLWDEVLEELAPQYEGVAAER
                     QHCDALIMNLVRWPERFDVIVASNLFGDLLTDLGGVIGGGLGLAPSTNTNPERRFPSM
                     FEPVHGSAPDIAGRGIANPLAAILSAAMMLDHLQLPAAAQRIRRSVERTLADGNQTPD
                     LGGRLTTKEMGAAVVQRLDH"
     gene            2724..3245
                     /locus_tag="VFB80_09140"
     CDS             2724..3245
                     /locus_tag="VFB80_09140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZN33971.1"
                     /translation="MATLDLKKYIRDIPDFPKPGILFRDITPLLGNAAAFRESVRRMA
                     DPYREKKIEAIVAAEARGFIFAGPMAIELGAGFVPVRKPGKLPFDTHSFRYDLEYGSD
                     TLEMHVDGVGAGTRVLVVDDLLATGGTVGACVKLLERTGATIVGCAFAIELAALGGAA
                     RLDPHPVYSLIKY"
BASE COUNT          545 a         1161 c         1048 g          512 t
ORIGIN      
        1 cagaacacgc ggagtttgtc cggcgtctcg cagctcgcaa gtaaccggtc tccatgcccc
       61 acgaccgcta cgacaacccg ctcagcagcc gctatgcctc gccggaaatg tcggcgctgt
      121 ggagcgagca gcggaaattc agcacctggc ggcggctgtg ggtcatcctg gccgaggccg
      181 agcgcgagct cggtctgccg atcaacgacc agcaaatcgc ggagctgcga gcccacaccg
      241 acgacatcga tttccaggcg gccgccgcgt acgagaaaaa gctgcggcac gacgtgatgg
      301 ctcacgtgca cgcctacggc gacgtctgcc ccgcggcgcg gcccatcatt cacctcggcg
      361 cgacgagctg ctacgtcacc gacaacgccg acctgctgct gatgcgcgag ggcttgcagc
      421 tgctcgcccg ccggacggct gccgtcatcg accagctcgc acgcttcgcc gcggagcacc
      481 gcgatctgcc ctgtctgggc ttcacccacc tgcagccggc acagccgacg accgtgggca
      541 agcgggcctg cctgtgggcc tacgacctcg cgctggacct ggcggagctg gagcatcgca
      601 tcgcctcgct gaagtcccgc agcatcaaag gcaccaccgg cacgcaggcc agcttcctcg
      661 ctctcttcgg aggcgaccac gccaaggtcc gccgcctcga aagactcgtc gcccagaaga
      721 tcggctttga gagcagctat gccgtcacgg ggcagaccta ctcgcggaaa gtcgattcgc
      781 aggtcctcga cgccctctcc ggtctcggcc aatcggccca caagctggcg accgacctgc
      841 ggctgctcgc ttcgcgcaag gaaatggagg agccgttcga ggccgatcag atcggcagct
      901 cggcgatggc ctacaagcga aatccgatgc gcagcgagcg ggtctgcgga ctggcgcggt
      961 tcctgatgag catgcagtcg aacgcggcgg ccaccgcggc ggtgcagtgg atggagcgga
     1021 cgctcgacga cagcgccaac cggcggctgg tgatcccgca ggcctttctc gccgccgatg
     1081 cgatcctcct cttgcttcag aacatcacta gcggcctggt cgtctatccc cgctcgatcg
     1141 cggcgaacct ggccgccgag ctgccgttca tggccaccga gaacatcctg atggcggccg
     1201 tcagcgccgg cggcgaccgg caggacctgc acgagcggat ccgccgccac agccaggccg
     1261 ccgcgctccg cgtcaagcag gagggattgc ccaacgacct gctggagcgg ctgcgtcacg
     1321 acccggcctt tgccaaggtc gatctggctt cggtcaccga ggcgcgccag ttcgtgggcc
     1381 gtgcgccgca gcaggtcgat gagtttctgg cggacgtcgt cgcacccatc cgcgcccgct
     1441 atcccgccgc ggcaggaccg ccggcggaaa tcagcgtgta agcttctcga catgaaaacc
     1501 tcgctcaaaa tcgccgtcta tgccggcgac ggcatcgggc ccgaagtgat cgacgaggcc
     1561 ttgaatgtgc tcgccgccgt gcaagcgctg gacggctcgt tccagctcga gttcacgcgg
     1621 ctgaactggg gcgccctgca ctaccggcag caccagacgg tcgtgcccga cgacttcctc
     1681 gacgtgctgc ggccgctgga cgcgatcctg ctcggggccg tcggctggcc ggcgctgatt
     1741 ccggaccaca tcacgctcgc gccgctcgtg aaaatccggc agtcgttcga tcagtacgcc
     1801 tgcgtccggc cgagcaagct ctacccgggc gtgaagtccg tcctcgccgg caagggcccg
     1861 gccgacatcg atttcgtcgt catccgcgag aacagcgaag gcgaatacgt cgataacggc
     1921 ggccggctga agcgcggcac gcccgacgag gtcgccgtgc agtcggccct ccacagccgc
     1981 aagggggtcg agcggatcct ccgctacggc ttcgacctgg cccgccggcg gcgcagtcgg
     2041 ctgacgatgg ccaccaagag caacgcccag cggcacgcct atgtcttgtg ggacgaagtc
     2101 ctggaggagc tcgcgccgca gtacgagggc gtcgccgccg agcggcagca ctgcgatgcc
     2161 ttgatcatga atctcgtccg ctggccggag cggttcgacg tcatcgtcgc gtccaatctg
     2221 ttcggcgacc tgctgaccga cctgggcggc gtcatcggcg gcggcctggg cctggccccc
     2281 agcaccaaca ccaatcccga gcgccgcttt ccttcgatgt tcgagcccgt gcatggcagt
     2341 gcgcccgata ttgccggccg cggcatcgcc aatccgctcg cggccatcct cagcgccgcc
     2401 atgatgctcg accatttgca gctgccggcc gccgcccagc gcatccgccg ctccgtcgag
     2461 cgcacgctcg ccgatggaaa ccagacgccc gacctcggcg gccggcttac gaccaaggaa
     2521 atgggcgctg cggtcgtcca gcggttggat cattgagttt ccctggccga tcgtttaacc
     2581 cggagccgga ggcgacggcg cccactggga aaagcctgcg cctccggcta cgggttaaac
     2641 gagccatcga cagccctccc caatccactc cgcgatcgtg ctatattcac cgcgacgaag
     2701 aactccttcc ctcagccgtc tccatggcca cgctcgacct gaagaagtac atccgcgaca
     2761 tccccgattt tcccaagccg gggatcctgt tccgcgacat cacgccgcta ttgggcaacg
     2821 ccgccgcctt ccgcgagtcc gtccgccgga tggccgatcc ctatcgcgag aagaagatcg
     2881 aagcgatcgt ggcggcggaa gcccgcggct tcatcttcgc cgggccgatg gcgatcgagc
     2941 tcggcgcggg tttcgtgccc gtgcggaagc cggggaagtt gccgttcgac acgcactcgt
     3001 tccgctacga tctggaatac ggcagcgata cgctggagat gcacgtcgat ggcgtcggcg
     3061 cgggcacgcg ggtgctcgtc gtcgatgacc tgctggccac cggcggcacc gtcggggcct
     3121 gcgtcaagct gctggaacgc accggcgcga cgatcgtcgg ctgcgcgttc gccatcgagc
     3181 tcgcggccct cggcggggcg gccaggctcg atccgcatcc ggtctatagc ctgatcaagt
     3241 attgaccgag gcgagcgagg ggcgtg
//
LOCUS       DASRJB010000347         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2919567, whole genome shotgun sequence.
ACCESSION   DASRJB010000347 DASRJB010000000
VERSION     DASRJB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919567"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..631
                     /locus_tag="VFB80_09145"
     CDS             131..631
                     /locus_tag="VFB80_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33972.1"
                     /translation="MSNPLLRPNDPRFHKPEVRDEAGQNRFGDTQPAGEAKAADAGVF
                     AAAAVEEARPYQPRYEAQQSARGGLLMILAGGGLVCGLLGMIALLEVLRLGWVFPLLG
                     LGPAAAAWLLAHEDLKAIAVGAIDETGRPRTRRAFWLGLAALLLCLGVVGSMVWRQMN
                     FLPAIL"
     gene            complement(1139..1765)
                     /locus_tag="VFB80_09150"
     CDS             complement(1139..1765)
                     /locus_tag="VFB80_09150"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HZN33973.1"
                     /translation="MAASSLPSAAPFVVAITGASGAVYAVRLLEVLLSSGRELHLMIS
                     PSGAAVIRQELDRAVSLEQFDARSLLPAAAAEQAAGLIRYHHYSDFMAPPASGSFLTG
                     GMVVCPCSGSTLSGIAHGSSTNLIQRAADVHLKERRKLILVPRETPLSLIHLENMKLA
                     AAAGAVILPAMPGWYHGVRSVQDLIDFVVARILDQLGLPHELMRRWGQ"
BASE COUNT          363 a          635 c          679 g          381 t
ORIGIN      
        1 tcgtctaccg catgcggttc gacgaagcca gtgcccgctt cgccgaattc ggccccttct
       61 acaccagcta catcaccact cccgccgaaa tgctcgacca ttgccggctt tcggcgaggt
      121 agatcttgcg atgtccaacc cgctcctcag gccgaatgac ccgcggtttc acaagccgga
      181 agtccgcgat gaggcgggcc agaaccggtt cggcgacacg cagccggcgg gcgaggcgaa
      241 ggcggcggac gccggcgtgt tcgcggctgc ggccgtggag gaggcccggc cctaccagcc
      301 gaggtacgaa gcccagcaat cggctcgcgg ggggctgctg atgattctgg ccggcggggg
      361 gctggtgtgc gggctgctcg ggatgatcgc gctcttggaa gtcctgcggc tgggctgggt
      421 gtttccgctc ttgggcctgg ggccggcggc cgcggcgtgg ctgctggccc acgaagacct
      481 gaaggcgatc gccgtcggcg cgatcgacga aaccggccgg ccgcgcacca ggcgggcctt
      541 ctggctgggg ctggctgccc tgctgctctg cctgggcgtc gtcggctcga tggtctggcg
      601 gcagatgaac ttcctgccgg cgatcctcta gctttccaaa tggaccacac tagtccattt
      661 cgcgctcgag cgcgaaaaaa acttacggcg acgcgccgag ttggaagtgg ctggctgagt
      721 tgaagttgcg gcgacgcgca ttgacgcaac tacgcatgta atggtcaaaa ttggatcaag
      781 tagcttgaca gccgcggccg gatatggata attgtgtcca cgtgtggata tcggaattca
      841 gttgccagcc cgagcatcct gggccgcacg tggctctttc gcaattcgga tggcccgctc
      901 gcgccggcgg ctgcggccga tcggaacttt ccccgatcgc cgctccatgc agtgcagatg
      961 acgctgccaa gcgccggcgc gcgacaaaaa cagcgggtct caaaggctaa taagtagtac
     1021 ttacgggttt ttcggactcg cgtcacaaat cattgctctg caatgccttg tggcgaaatt
     1081 cggccgcgaa gggcaagttc agggcaccga ttcaggggga ttagaagcaa ccgcccgctt
     1141 actggcccca gcggcgcatc agctcgtgcg gcaggccgag ctggtcgagg atccgggcga
     1201 cgacgaagtc gatcaggtcc tgcaccgacc tgacgccgtg gtaccagccg ggcattgccg
     1261 gcaggatcac cgcgccggcg gcggcggcca gcttcatgtt ctcgaggtgg atcagcgaca
     1321 gcggcgtctc gcgcggcacg aggatcagct tccgccgttc cttgaggtgc acgtcggccg
     1381 cccgctggat caggttggtg ctcgagccgt gggcgatgcc gctgagcgtg ctgcccgagc
     1441 agggacaaac gaccattccg cccgtcagga acgaaccgct ggccggcggc gccatgaagt
     1501 cggagtaatg gtgatagcga atcagcccgg ccgcctgctc cgcagccgcc gcaggcaaca
     1561 gcgaccgcgc gtcgaactgt tccaggctga ccgcgcggtc cagctcctgc cggatcaccg
     1621 ccgcccccga cgggctgatc atcaggtgca gctccctgcc ggacgacaac agcacctcca
     1681 gcagccgcac ggcatacacc gcgccgctgg ctccggtgat ggcgacgacg aaaggtgcgg
     1741 cgctaggtaa actactcgct gccattattc aaattaggtt gcggccgaga tttgatatga
     1801 cccgtggaga gatttggtcc gaactgcgag tggcatggcg ttggttttgg ctctggatgt
     1861 tcgtcaagtg gcggaggttt tggctaccgc gacgacgcgt cacggtcaat tttggcgcaa
     1921 cgcgagttac tttgcattgc tttaacgatt tgtccgagcc cagcgcaatt tgggaaggaa
     1981 aggcgcactt ttcgttttgg ggctcgcgcc tgccgtttca tatccacaca tctgggaccg
     2041 aaccagacct gcagtcga
//
LOCUS       DASRJB010000348         4706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3199649, whole genome shotgun sequence.
ACCESSION   DASRJB010000348 DASRJB010000000
VERSION     DASRJB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199649"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3792)
                     /locus_tag="VFB80_09155"
     CDS             complement(<1..3792)
                     /locus_tag="VFB80_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN33974.1"
                     /translation="MLTCPHCKATLSPAQVAQQQCPHCQRAIQTGEATIDFGRGAPTI
                     DGPSPGQQAKPATVDNLAEVTFDLGAPGSVGSTAKPDSHSQTHDSTALPQAATPSSGD
                     SPSASGGSSGSAHDQTLDSTSRDSAIGSRPGGSHGSSPTIDSVALPARKGGSTLAERE
                     HAQTVDRPSGDVDVEQQLADLWGSSQDASRPNVSMRGSSAARSSVEENSTLVIRERAL
                     RGQNDLKLAMADYDLLGVLGEGGMGVVYNARQASVDREVAVKMLKPEMLNSEQQRRKF
                     LSEAAVTGELDHPNIVPIYDLGQNADGALFYSMKKVQGTPWSKVIGQKSLLDNLSILM
                     KVSDAVAFAHARGVVHRDLKPENIMLGDFGEVLVLDWGLAVATELHRPTPNVSMAYNI
                     AGTPAYMSPELAMGPLERIGAHSDIYLLGAILYEIITGKPPHSGRSARDCLKAAARNK
                     INPTEKRGELVEIAKKALATDPGDRYPTVQALQEAIRQYQAHSESIALCERAEQELKA
                     AHQSDDYRDFARALFAFEEAHNLWTGNQAALAGISRAKLHYAASAHRKHDYDLGLSLV
                     DPQQADHAEIREQLVASKRERDARQQRLRRARQVVASLAAAVFLAVTIGIILVTRFWL
                     EADKQKENAVAQTKIAVREKQTADTERNNARINEARAKAEQQKAEYEAYAALISMASA
                     KIDEHAFDQARQALVACQQLAAGRENLLGWEWRRLWYVVNQGNVVDLELPAAAECLAI
                     SSQGDLIATGSLDGVIRVFDAQTRQLAVEQPVVGKTAKICALAFCPSDESWLAIGTSD
                     PQQPLILWNRQTKEIQPLGGPDGHSAAVVDVAFSRDGAKLLTASHDHTAKLWDRKTRQ
                     PLVTYRGHSWWVWSAALSPDERQVATASDDGTVRLWNAATGVEWTDAAGERKPFRVHQ
                     GPVYAVAFQPPPAGAPAADASGPARGLWIASAGYDNRVLLWKPEDLTAFDFTQLLKEG
                     EIRKTPCLELRGHTAAVRDLAFSADGQRLLSASSDHTLCIWYGREEDAGYAFIRAGTL
                     DKQLRGHTGSIQAAAFSPTNPRQVVSASSDGRVKLWTTSSYHEQQVLPGIALRGHLGA
                     VMSARYGRDGQSIVTASSDRTAKKWVFQEGAAREALTFREGHSYLTSRAIFFPDGQRL
                     LTAAVDGTVRIWSRATQAELLKLEGTGYRAAAALSPDGSWILTGTHGQGAILWDAATA
                     QPRHELLGQHKVSVGAVAFSPDGRLAFTGDDNGVGNLWEVATGRHVARLSFHLGPISA
                     AA"
     gene            complement(3834..>4706)
                     /locus_tag="VFB80_09160"
     CDS             complement(3834..>4706)
                     /locus_tag="VFB80_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33975.1"
                     /translation="GAIVRFGAIEAKISYEGGPLGNSVTLTVTDQLKIVRTNSEVAAL
                     GVIVRTAEPQLLMQRPQSAAAGLLLTESTAAVANADTVDRPPAAAEFRAAQRLRVYFR
                     VVNEGTAVEDPKEHELDPATALRDVLGIFKRLAFPNGRYRLYLQEPGRRERLIVEVNI
                     VDGKIVPQNFRETESQGAPLLPQTEAAPAPPPAGQPPAPAQPAAGNAEQPAAGNAVQP
                     ASGAIAVSHDRDSSAASLMGVGFGLAATAPVWLDRVQKVIAQGGALPRGRFSRLLRRL
                     AAAPFAGPRMVQNP"
BASE COUNT          719 a         1536 c         1619 g          832 t
ORIGIN      
        1 cgccgcggcg gaaatggggc caagatggaa actgagcctc gcgacgtggc ggcctgtggc
       61 tacttcccac aggttgccga cgccgttgtc gtcgcccgta aaggcgagcc ttccatcggg
      121 cgagaaggcc acggctccca cggagacctt gtgctgcccc aatagttcgt ggcggggctg
      181 agccgtcgcc gcgtcccaga ggatcgctcc ctgcccgtgt gttccggtga gaatccagct
      241 gccgtcaggc gagagggccg ccgcggcgcg gtagcccgtg ccctccagct tcagcaattc
      301 ggcctgagtc gcgcggctcc agattcgcac tgtgccatcg acggcagccg tgagcagccg
      361 ctgtccgtcg ggaaagaaga tggcccgcga ggtgagataa gaatggcctt cgcgaaaggt
      421 gagcgcctca cgggcagcac cttcctggaa aacccacttc ttggccgtgc ggtcgctgct
      481 ggccgtgacg atgctctggc cgtcacgccc ataacgggcc gacatcaccg cacccaggtg
      541 gccgcggagc gcaatgccgg gcagcacttg ctgctcgtga tacgagctgg tcgtccagag
      601 cttgacgcgg ccgtcggaac ttgccgagac gacctgccgc ggattggtgg gcgagaacgc
      661 ggccgcctga atcgatcccg tgtgaccccg cagttgcttg tcgagcgtgc cggcccgaat
      721 gaaggcgtag cccgcgtcct cttcgcggcc gtaccagatg cacagcgtgt ggtcgctcga
      781 agcgctcagc agccgctggc cgtcggccga gaacgccaag tcgcgcaccg cggccgtgtg
      841 cccgcgcagc tccaggcacg gagtcttgcg gatctcgcct tccttcagga gctgagtgaa
      901 atcgaatgcc gtcaaatcct ccggcttcca gagcagcacg cgattgtcgt agccggcgga
      961 ggcgatccag agtcccctcg cgggcccaga cgcatcggcg gctggagcgc cggccggcgg
     1021 cggctgaaac gccacggcgt acacgggacc ctgatgcacg cggaagggct tgcgttcgcc
     1081 ggcggcgtcg gtccattcga ctcccgtggc cgcgttccac agtcgcaccg tgccgtcgtc
     1141 gctggccgtc gccacctgcc tctcgtcggg cgaaagggcc gccgaccaga cccaccagct
     1201 gtggccgcga taagtgacca gcggctggcg cgtcttgcgg tcccacagct tggccgtgtg
     1261 atcgtgcgac gcggtgagca gctttgctcc gtcgcgcgaa aacgcaacgt ccaccacggc
     1321 agccgaatgc ccgtcggggc cgcccagcgg ctggatttcc ttcgtctggc gattccacag
     1381 gatcagcggc tgctgcggat cgctagtacc gatcgccagc cagctttcat cggaggggca
     1441 gaaggcgagg gcgcaaatct tggccgtctt gccaacgacc ggctgttcga cggcgagctg
     1501 gcgagtctgc gcatcgaaca cacggatcac gccgtcgagg ctgcccgtgg cgatcaggtc
     1561 gccctggctg ctgatcgcca ggcactcagc agccgcgggc agctcgagat cgacgacgtt
     1621 gccttgattg acgacatacc acagccgacg ccactcccag ccgagcaggt tttcgcggcc
     1681 cgcggccagc tgttggcagg ccacgagcgc ctggcgagcc tggtcgaagg cgtgctcgtc
     1741 gatcttggcc gaggccatgc tgatcaaggc cgcgtaggct tcgtattccg ccttctgctg
     1801 ctcggccttg gcccgagctt cgttgatgcg ggcattgttt cgctcggtgt cggctgtttg
     1861 cttctcgcgc acggcgatct tcgtctgggc gacggcgttt tccttttgct tgtcggcctc
     1921 gagccagaac cgcgtgacca ggatgatgcc gatcgtgacg gcgaggaaca ccgcggcggc
     1981 cagggaagcg acgacctggc gggcccgccg cagccgctgc tggcgcgcgt cgcgctcgcg
     2041 cttggaggcg acgagctgct cgcgaatctc ggcgtggtcg gcctgctggg gatcgacgag
     2101 cgacaggccc agatcgtaat cgtgcttgcg gtgggcgctg gccgcgtagt gcagcttggc
     2161 gcgcgagatg ccggccagcg cggcctgatt gccggtccac aggttgtggg cctcttcgaa
     2221 ggcgaacagc gcccgggcga agtcgcggta gtcgtcggac tgatgggcgg ccttgagctc
     2281 ctgctcggcc cgttcgcaga gggcgatgct ctcggagtgg gcctggtact ggcggatcgc
     2341 ttcctggagc gcctgcacgg tgggatagcg gtcgccggga tccgtagcca gggccttctt
     2401 cgcaatctcg accaattcgc cgcgcttctc ggtcgggttg atcttgttgc gggcggccgc
     2461 cttcaggcag tcgcgggcgc tgcggccgga gtgcggcggc ttgccggtga taatttcgta
     2521 cagaatcgcg cccaagagat agatgtcgct gtgcgcgccg attcgttcga gcggacccat
     2581 cgccagctcg ggcgacatat aggccggcgt cccggcgatg ttgtacgcca tggaaacgtt
     2641 gggggtcggc cggtgcagct cggtcgccac cgccaggccc cagtcgagca cgagcacctc
     2701 gccgaagtcg ccgagcatga tgttctcggg cttcagatcg cggtgcacga cgccgcgggc
     2761 gtgagcaaag gccaccgcgt cggacacctt catcaggatg ctcaggttgt cgagcagcga
     2821 cttctgtccg atgactttcg accagggagt gccctggact ttcttcatcg agtagaagag
     2881 cgcgccgtcg gcgttctgac ccaggtcgta gatcgggacg atattcggat gatcaagctc
     2941 gccggtgaca gccgcctcgg agaggaactt gcggcgctgc tgctcgctgt tcagcatctc
     3001 gggcttgagc atcttgaccg ccacttcccg gtccaccgac gcctgacggg cgttatagac
     3061 gactcccatt ccgccttcgc ccaatacgcc gagcaggtcg taatcggcca tcgccagctt
     3121 cagatcgttc tggccgcgga gggcccgctc gcgaatgacg agcgtcgaat tctcctccac
     3181 gctgctcctg gcggccgagc tgccgcgcat ggaaacgttt ggccgcgaag cgtcctgcga
     3241 cgagccccag aggtcggcga gttgttgctc gacatcgacg tcgcccgaag gccgatcgac
     3301 cgtctgggcg tgctcccgct cggccagcgt gctgccgccc ttgcgtgccg gcagggcgac
     3361 cgagtcgatc gtgggggacg agccgtggct gccgccgggc cggctgccga ttgcagaatc
     3421 acggctggtg gaatcgagcg tctggtcgtg ggccgaaccg ctgctgccgc cgctggcaga
     3481 tggcgaatcg ccgctcgatg gcgtggctgc ctgaggcaac gctgtggaat cgtgcgtttg
     3541 agagtgcgaa tctggtttcg cggtgctacc gaccgagcca ggggccccga ggtcgaacgt
     3601 gacttcggcg aggttatcga ccgtcgcggg cttcgcctgt tggccgggcg atggcccgtc
     3661 gatcgttggc gccccgcggc cgaaatcgat cgtggcctcg ccggtttgga tcgcgcgctg
     3721 gcaatgcggg cattgctgct gggcaacctg cgcaggcgag agcgtggctt tgcaatgggg
     3781 acaggtaagc acgagggcgg ctccaacgtg tcgggctctc gcccgaactg cgatcaggga
     3841 ttttgaacca tgcgtgggcc tgcgaacggg gccgcagcca gcctgcgaag cagtcgcgag
     3901 aatcttcctc gcggcagagc gccgccctgc gctatgactt tttggacgcg atcgagccag
     3961 accggagcag tcgccgccag gccgaatccc acacccatta gcgaggcggc cgacgagtcg
     4021 cgatcatggg aaacggcaat tgcacccgat gcgggctgca ctgcattccc ggccgcgggc
     4081 tgctcagcat tcccggccgc gggctgcgca ggcgccggcg gttgaccagc gggcggcggc
     4141 gcgggagccg cctctgtctg cggcagcagg ggtgcgccct gggattccgt ttcgcggaag
     4201 ttctgcggca cgatcttgcc gtctacgatg ttcacttcga caatcaaccg ctcgcgcctg
     4261 ccgggctctt gcaagtacag gcgatagcgg ccgttgggaa acgccagccg cttgaagatg
     4321 cccagcacgt cgcggagggc cgtcgcggga tcgagctcgt gctccttcgg atcctcgact
     4381 gccgtgcctt cgttgaccac gcggaagtag actcgcaacc gttgggccgc gcgaaactcg
     4441 gccgcggcag gcggacgatc gaccgtatcg gcgttcgcca cggcggcggt ggattccgtc
     4501 agcaacaatc cagcggccgc cgactgcggg cgttgcatca gcagctgagg ctcggcggtc
     4561 cgcacgatca cgcccagtgc tgcgacctcg ctattcgtgc ggacgatctt gagctgatcg
     4621 gtgacggtga gcgtcacgct gttgcccagc ggcccgccct catagctgat cttggcctcg
     4681 atcgcgccga agcggacgat cgcgcc
//
LOCUS       DASRJB010000349         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2354974, whole genome shotgun sequence.
ACCESSION   DASRJB010000349 DASRJB010000000
VERSION     DASRJB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354974"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFB80_09165"
     CDS             complement(<1..185)
                     /locus_tag="VFB80_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33976.1"
                     /translation="MADRDALLGRETAPLLAAGLIAGGMSLVALLVYVQPTRHDAAWR
                     GVMLGTLFGQVSLAAAWT"
     gene            complement(210..338)
                     /locus_tag="VFB80_09170"
     CDS             complement(210..338)
                     /locus_tag="VFB80_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33977.1"
                     /translation="MSPAAGEINYSRVLRAAKELGYTGFVGLELRPQERARQPPSP"
     gene            613..798
                     /locus_tag="VFB80_09175"
     CDS             613..798
                     /locus_tag="VFB80_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33978.1"
                     /translation="MKDENPYQSPQEAGYFPPRLLSVAIDWLCERMWKLVLGLLLSLV
                     AQPFVVAFLWIHGEFRL"
     gene            1149..1691
                     /locus_tag="VFB80_09180"
     CDS             1149..1691
                     /locus_tag="VFB80_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN33979.1"
                     /translation="MTPSPIDWQAALAQHDRWLRTVAVARLQEPQAVDEVMQEVALAA
                     VKQAAPLADAGKVAPWLYRLTVRQALLYRRKCGRRRRLAGRYADQVERQERGPPAAAD
                     PLGWLLAEERRELVRRALAGLAPKDAEILLLKYTEHWNYHQIAAHLGVSHAAVEARLH
                     RARGRLRQELAALDVVEVGR"
     gene            1688..2395
                     /locus_tag="VFB80_09185"
     CDS             1688..2395
                     /locus_tag="VFB80_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33980.1"
                     /translation="MIGRQDELTPRDGDARFDRLVDGELTAEEYRSLLAGLEHEPGGW
                     RDCALAFLEAQALGTELGSVRRALDVRPAAANKPATMHRPWWPKLGTLLAMAASFALA
                     FGLGVTLPRIWFGDPQDGMAGGNLPSELLANRDAPGDERLTVLRPIANVQVVVGGDDG
                     QQRSAGELPVYEIRQNLDEYLRESQPGLDPYVLDWLQRHHDVEVEQRLIPGRLDDGRP
                     MYVPVEQYTITPVKRSY"
BASE COUNT          445 a          782 c          904 g          455 t
ORIGIN      
        1 gtccacgctg ccgctagact gacctggccg aaaagcgtgc caagcattac gccacgccag
       61 gctgcgtcgt gccgagtggg ctgcacgtaa accagcaacg ccaccagact catgccgccg
      121 gcgatcagcc cggcggctaa aagtggcgcg gtttcgcggc ccagcagagc atcgcggtcg
      181 gccatcggtt caccagccgt cggcctgctt cacggcgagg gcggctgcct cgctcgctct
      241 tggggacgca gctccaggcc gacgaagcca gtgtagccca gctccttcgc ggcccgcagg
      301 acgcgactgt agttgatctc gccggcagcg gggctcattg cactcgcggc cgctctgaaa
      361 ggccagctga cgcctagaga aaggcatgac tggcttccat tttcttgaaa atagttgcaa
      421 ctccttgtgc ccgaaaagtt tgcgcgccgg cagtcggaga gttttgccgc tcgagaggtc
      481 cgaaaacggg cgaggtcatt gtataatctg tccactacaa aaacgaatgc tgcaatggtt
      541 cgctggcggc cgcgcgaggc atgcgactga cctgcgagcg atgggccgcc aagagccaaa
      601 ggaccgcgaa cgttgaaaga cgaaaacccc taccagtcgc cacaggaggc cgggtacttt
      661 ccgccgcgat tgctcagcgt tgccatcgat tggctgtgcg aacgcatgtg gaaactggtt
      721 cttggcctgc tgctgtccct cgttgcgcaa ccatttgttg tggccttcct atggattcac
      781 ggcgagtttc gcctgtaggt ccctcctgcc gggcgcggca cggtcgaacc ttggcaatgg
      841 gcgcggtcgc tgcgcttccc tgaaggtccc ttccggcaga agggacctac tttgaacgag
      901 gtatgcgaag ggtaaggata attccgcggg gacctgtcta actgttcaaa gcatcataag
      961 gaattcagtc tcccagatcg ctataagtgc atgctgggaa ctaacttgcg attgataccc
     1021 tgactttcag cgtttcgtgg ccgagggcta tcgggaatta tccttacctc ctctcggcgg
     1081 cccgcggcaa gaaccggctg ccagcggtaa cgtatccagg tcgggcgaga ggccaggctg
     1141 atcgtcacgt gactccaagc ccaatcgact ggcaagcggc gctcgcgcag cacgatcggt
     1201 ggctgcgcac cgtcgctgtt gcgcgcctgc aggagccgca ggcagtcgat gaggtgatgc
     1261 aggaagtggc gctggcagcc gtgaagcagg cagcgccgct cgccgatgcg ggcaaggtgg
     1321 ccccgtggct gtatcggctg acggtgcggc aggcgctgct ctatcgccgc aagtgcggcc
     1381 ggcggcgacg actggcgggg cgctacgccg accaggtgga gcggcaggag cgcggcccgc
     1441 cggcggcggc cgatccgctg ggctggctgc tggccgagga gcgccgcgaa ttggtgcggc
     1501 gggccctggc cggcctggcg ccaaaggacg cggagatttt gctgctcaag tacacggagc
     1561 attggaatta tcaccagatc gccgcccact tgggcgtgag ccatgccgct gtggaggccc
     1621 gcttgcaccg ggcccgcggc cggctgcggc aggaactggc ggcgctggac gttgtggagg
     1681 tgggacgatg atcggccggc aggatgaact tactccccgc gacggcgacg cccggttcga
     1741 ccggctcgtc gatggcgagt tgaccgccga ggagtaccgg tcgctgctcg ccgggctcga
     1801 gcacgaaccc ggcggctggc gagattgtgc cctggcgttt ctcgaggctc aggcgctggg
     1861 gaccgagctg ggcagcgtgc ggcgagcgct cgacgtgcgg ccggctgcgg cgaacaaacc
     1921 ggcaacgatg catcggccgt ggtggccgaa gctcggcacg ctgctggcaa tggctgccag
     1981 ctttgccctg gcctttgggc tgggcgtcac gctgccgcga atctggttcg gcgatccgca
     2041 agatgggatg gccggcggaa accttcccag cgagctgctg gccaaccgtg acgcccccgg
     2101 cgacgagcgc ctcacggttc tgcggccaat cgccaacgtg caggtggtgg tgggcggcga
     2161 cgacggccag cagcgaagtg cgggcgagct gccggtttac gagatccggc agaacctcga
     2221 cgagtacctg cgggagagcc agccgggcct cgatccgtac gtgctggatt ggctccagcg
     2281 gcatcacgac gtggaggtgg aacagcggct gattccgggc cggctggatg acggccggcc
     2341 gatgtacgtg ccggtcgagc agtacacgat cacgccggtg aagcgatcgt attagtcagt
     2401 tgtctggtca gttgggggca cgcaacggga cagggagtgt tccttactca tctttttcaa
     2461 aggggtcgag attatgaagt catcgtggaa taagtggctg gcgtgcggcc tggtcgccgc
     2521 gtcggccgtg tgggctggcc cgtgggccag cggacagtct gtgcccgtgc aagtgcggcg
     2581 gccccg
//
LOCUS       DASRJB010000350         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_949453, whole genome shotgun sequence.
ACCESSION   DASRJB010000350 DASRJB010000000
VERSION     DASRJB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949453"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFB80_09190"
     CDS             complement(<1..257)
                     /locus_tag="VFB80_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33981.1"
                     /translation="MFTNITVRRPRRQRPPSARDQALFFAYLLQGKSQAELALECCLS
                     QARVSQIIRRVRAWRHQLSTEEQRAHQELYETVYRTAMRKLL"
     gene            840..>2297
                     /locus_tag="VFB80_09195"
     CDS             840..>2297
                     /locus_tag="VFB80_09195"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HZN33982.1"
                     /translation="MAKSVEAVAQAGARRELVFQDGSSNKFWNIELSGSSFTVTFGRV
                     GTAGQEQTKEFADDDAAQKAYDKLVAEKVKKGYVDAGGSASTAKPAANQTAKPAAKQT
                     AKPAAKPAAAKPPKAKAKSADEPAATNGAGAAAPAPAAAADLSTKRQLDLEPADWARA
                     TFRDRKVLARGEPQPFDQEAAAGRMAKLRTEYYGWHVKWRDLNLPAALSPEEAHFWLE
                     AMTLPRERNDSLQKQAEKLAKKKFSGKVAAADVVNVLTRNPHGLPDEASLAIANLLSA
                     EEYLELLILCKPQARDYQLAAALGSLMQGFSKYVYPYLTDAQVQAARKPLLKNWDPTK
                     TPADLYEPLPIAYYLAAVLGMHEEVYAVTSTWPDDRYTKEDWADHYHRPQDVVFGLGS
                     PDLVASEWRRMKLRMRTPEHVRAFLACTEYDALDCVRDSILAETNKEKCELLVEAFTL
                     VSAPEAAAPMLECKLSCKTPSGAREWLDQHVGHAVA"
BASE COUNT          453 a          723 c          765 g          356 t
ORIGIN      
        1 agcagcttcc gcatggccgt gcgatagacc gtctcgtaca gctcctggtg cgcccgctgc
       61 tcctcggtgc tgagctggtg ccgccaggcg cggacgcggc ggatgatctg cgacacgcgg
      121 gcctgcgaca ggcagcactc aagagccagc tccgcctggg atttgccctg gagcagatag
      181 gcgaaaaaca gcgcctgatc gcgggcgctg ggcgggcgct ggcggcgtgg gcggcggacc
      241 gtgatgttcg tgaacacagg attctccttg cagcgagccg acagccaggc ggcggggccg
      301 cgagtggacc atccacccgc ggcgcacggc ggcccgatcg gcacccagca atttggcaag
      361 cggatctgtg tctgaaaagc aacttgtgta cattttaggc aaacaccttt ccgccggtgt
      421 catcgcaagg gcagatagag tacagactta caaaaacgct gctcgccgta agtcgtgaaa
      481 aaaacttttt ttcgcgcgct ggaggaatgg actagtgtgg tccattctgg aggtcccacg
      541 accgggggag cgcagggaat tccggggtcg ggagtctttg gcgggagagg gttgccgcaa
      601 tggcgatctg aatccgcgcc aacgactccc gactccgggc tagcgctgaa tcagtttctt
      661 agcccctgtt aggggcggca ggctcgggcg aggttatgcc tgaagaaaca accatccccg
      721 catgagtcgc gccattttct aagggccgcc ggccagccgc aactggtcct gtcgcttgac
      781 gcctgcggcc ccactacatt agttgccact cctcctcgat cgatttgcgg agagccccca
      841 tggcgaaatc agtggaagca gtggcgcagg ccggcgcgcg gcgggaactc gtctttcagg
      901 acggcagctc gaacaagttc tggaacatcg agctctcggg aagctcgttc acggtcacgt
      961 tcggccgcgt cggcacggcc ggccaggagc aaacgaagga gtttgccgac gacgacgcgg
     1021 cccagaaggc gtacgacaag ctcgtcgccg aaaaggtgaa aaagggctat gtcgatgcgg
     1081 gcggcagtgc gtccaccgcg aagcccgctg ccaaccaaac cgcgaagccc gctgccaagc
     1141 aaactgcgaa gccggcagcc aaaccggccg cggcgaagcc ccccaaagcc aaggcgaagt
     1201 cggccgatga gcccgcggcc accaatggcg cgggcgcagc agcgccggcc ccggcggccg
     1261 cagccgacct ttccaccaag cggcagctgg acctggagcc ggccgattgg gcgcgggcca
     1321 ccttcaggga ccggaaagta cttgcccgtg gagagccgca gccgttcgat caggaggctg
     1381 ccgcggggcg aatggccaag ctccgcaccg aatactacgg ctggcacgtg aaatggcggg
     1441 acctgaacct gccggccgcc ctgtcgccgg aagaggccca tttctggctg gaggcgatga
     1501 cgttgccgcg ggagcgcaac gactcgctcc agaaacaggc cgaaaagctg gccaagaaga
     1561 aattcagcgg caaagtggcg gcggcggacg tagtgaacgt gctgacccgc aacccgcacg
     1621 gcctgccgga tgaagcctcg ttggcgattg cgaacctgct ctccgccgag gagtatctgg
     1681 agctgctcat cctgtgcaag cctcaggcgc gggattatca gctcgccgcg gcactcggca
     1741 gcctgatgca gggctttagc aaatacgtct atccatacct gaccgacgcg caggtccagg
     1801 ctgcccgcaa gccgctgctg aagaactggg atccgaccaa aacgccggcc gatttgtatg
     1861 agccgctgcc gatcgcgtat tacctggccg ccgtgctggg gatgcacgaa gaggtgtatg
     1921 cggtgaccag cacctggccg gacgaccgct acacgaagga agactgggcc gatcactatc
     1981 accggccgca ggacgtcgtc ttcggcctcg gctcgcccga cctggtggcc tccgaatggc
     2041 ggcggatgaa gctgcggatg cggacgcccg agcacgtgcg ggccttcctg gcttgcaccg
     2101 agtacgacgc gctcgattgc gtgcgagatt cgatattggc cgagacgaac aaggaaaagt
     2161 gcgaactgct cgtcgaggct ttcacgctgg tcagcgcgcc ggaggcggcc gcgccgatgc
     2221 tggagtgcaa gctctcctgc aaaaccccga gcggcgctcg cgagtggctc gaccagcacg
     2281 tcggccacgc cgtggcc
//
LOCUS       DASRJB010000351         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_949766, whole genome shotgun sequence.
ACCESSION   DASRJB010000351 DASRJB010000000
VERSION     DASRJB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949766"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFB80_09200"
     CDS             <1..298
                     /locus_tag="VFB80_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN33983.1"
                     /translation="LGRQPFTARRFFLKYQDRILFGTDGPWPEERIRLYWRFLETEDE
                     YFPYSEKPFPPQGLWNIFGLNLPDEVLRKVYHENALRLIPGVREKFTRAFAALP"
     gene            complement(999..2108)
                     /gene="ribD"
                     /locus_tag="VFB80_09205"
     CDS             complement(999..2108)
                     /gene="ribD"
                     /locus_tag="VFB80_09205"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZN33984.1"
                     /translation="MASSFDSEVMQRAITLARRGEGFVEPNPLVGCVIVGEGQVVGEG
                     WHQRFGGPHAEIEALRAAGERARGATLYVTLEPCCHHGKTPPCTEAVIAAGIRRVIAA
                     MSDPFPQVAGGGLAQLADAGITVETGLHEAEARALNAPYLKLLATGRPWVIAKWAMTL
                     DGKLATRTGDSQWISSPASRAVVHRLRSRVDAIVVGRGTAERDDPLLTARLENAQPPR
                     VSTRIVLDSHASLRTDSQLVRTARQYPTLIAAGPDAADSDESRLTAAGCEVLRLGQSA
                     PVERLHALLAELGRRRMTNILVEGGSEVLGAFFDAKQIDEVHVFIAPKLIGGAIARSP
                     LGGGGIAAVAQALLLKQLLVRELEGDVYVSGRPAT"
     gene            complement(2181..4373)
                     /locus_tag="VFB80_09210"
     CDS             complement(2181..4373)
                     /locus_tag="VFB80_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33985.1"
                     /translation="MSLDPYQPCPCGSGKKNKFCCGPEVLADLAKIDEALAGEQRLAA
                     LDLCNRLLEKKPDQPAILVHKATVQLALNELDTCRETVARLLKVMPTSAAGLAIASIL
                     DCQEGKAEEAVDKLQTALENQQGQLLPVVYEALGIVSRTLNAVGEPLAAQTYAVFQAA
                     ASQGKDRNAVMNLLELESGGQISLAVHGMTGMVSAPADGALSAAGIAEFNEALKQADL
                     GCWRAAAKLFEALAQKFPQEPAVWRNMAVCKLRVIDNAGARAALRKFAALPAVPRDDA
                     VEAEGLAQYLSDLSEVDTVPEITVTIAVTDAQALREQLLSNKRVQSIPFDPAQFREAD
                     EPPPLFGFLLLDREVPAHSASLSRDNVPKVLGEVLLYGKETDRPARVEFAAVKTADFE
                     NRIKHLRDVLGPLAGEKLGEEETGRMPAVSAALAINWRFPDDTPPDFRKRMIQEHRTQ
                     TLLSIWPNLPMGVLDGRSPRQSVGDPFGQIRVQAVILQMDLAEPVENPDHNKLRRSLG
                     LPAAEPIDPSGVRVESLLPARQTRLAVEKLTDDQLHGVWRRAVIVAAPRLVRHIGLEV
                     VRRPSFAKRTDIDLAETYDILARMALDADEALSYLQKAQEILKAKGASPARFLLAELP
                     YRLQRGEEQESRRILNLLTTKHAREPGVQQALFNLLAQLGLVRMDPATGRPVMTVPAG
                     AVPGAAAAMPAGASAAPAPKSAVWTPDQGAPAAAGSEGKSKLWLPGMD"
     gene            complement(4501..6156)
                     /locus_tag="VFB80_09215"
     CDS             complement(4501..6156)
                     /locus_tag="VFB80_09215"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZN33986.1"
                     /translation="MIATADIESFVAAQPWEDDPTPVIDRISATLREAFGATFTFWDG
                     QTGELLHASTQQPASSDPFRGQLARAVHGNQPQFISDEDCVLLLAVPLPLSRDRTTVA
                     TAAFVVRPVAAHEHLAGPSHLLGLDQSKALAWILRQPIWSPEALLRLATAVQAQVRAE
                     EKTVQLEREVEKLSDNLAATYEEICLLHGVTQNLRIRSDEEQLCALVLRWLLDCIPTK
                     SVAIQLLPVAKEGQITYRARTRSVLLTAGQCPLDNQQFTQLIEDLNLEAGCGPLVANR
                     SVTGSASWRFAGVRELIVVPMTEGDRVFGWLAVLNHTDGGEFGTIEASLLNSVAAMLG
                     IHSSNRDLYRQQSEFLASVVRALTSAIDAKDQYTCGHSDRVARISVRLAQELNCEAEM
                     LHTLYMAGLLHDIGKIGIDDAVLRKPGKLTPAEFEHIKQHPELGYRILADIKQLADVL
                     PAVLHHHEQWDGRGYPFKLVGDQIPFIARIVAVADAYDAMTGDRPYRPGMPVERVEQI
                     FKNGAGQQWDPEVVKAYFVAKGDIQAIAGEERANLTLDVQQWT"
     gene            6362..>7552
                     /locus_tag="VFB80_09220"
     CDS             6362..>7552
                     /locus_tag="VFB80_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZN33987.1"
                     /translation="MDSLTAARLQMTVSLAFHMVYAAIGIGLPLLLVIVEGLYLRTGQ
                     PHYLALARRWAKVTGLLFAVGAVSGTALSLELGLLWPRYMELLGAVVGHIFGLEGYAF
                     FLEAIFIGLYLYGWERLSPLAHWWCGVVIAASGMLSGIFVLGVNAWMQVPVGFELAGG
                     RVVVTDPLAIFKHPLWAHMAWHSTLACYISVAFAVSGWYAWRLLRSPHDASFRAALLV
                     AMTVGSLAAVLQPLSGDYLAKYVFRTQPVKFAAMEGQFQTQTHAPLRLGGWPDEAAGK
                     TRWALEIPGGLSFLADGNPATEVRGLDQTPRADWPNVGLTHLAFQLMVGSGMAMVLVS
                     ILFWTTYFWRRQALFESRWLLWLVVLAGPLGFLGLEAGWFVTEVGRQPWIIQGVLRTR
                     EAVTP"
BASE COUNT         1171 a         2526 c         2537 g         1318 t
ORIGIN      
        1 gctgggccgg cagcccttca ccgcccggcg gttctttctc aaataccagg accgcattct
       61 cttcggcacc gacggcccct ggccggaaga gcgcatacgc ctctactggc ggttcctgga
      121 aaccgaggac gagtacttcc cctactccga aaagcccttc ccgccgcaag gcctctggaa
      181 catctttggc ctcaacctgc ccgacgaagt cctccgcaag gtctatcacg aaaacgccct
      241 gcggctgatc ccgggcgtgc gcgagaagtt cacccgcgcc ttcgccgcat tgccgtgaag
      301 ctggccaaag ccgcgaccag tggtcgcgcg ctacctcgcc gacgcgatca tttgcacgat
      361 tgttctgcga cactgcgatc catccgcgta gcggcgaagg agtgtagccg tgggctgacg
      421 cccacggctt tactctgccg ccgctgcgcg gctgcggcgc gatggccctt ctttcagggg
      481 ccgctaggag gcgcgaccac aataggttgt ggttgggcgg aggcggaaca caagatgttg
      541 tgttgcgctt gacatgtggt ggacggatag ttacaattcc acttggccgc gaggatcgtt
      601 cggcggcgac atggtatttg gcaaatcggt aaaacgcagc cggcctaatg ccgcgacgag
      661 ctctgaccgt gcagcgcgac acttgtcgcg ctctgccgac gcacaccctc gcttacgcgc
      721 ttgcgcgtcg ggttaagagt gcatcgcgac cactggtcgc ggctttgagg aagagccggg
      781 acgcgacgcg agacctctca aaccctaata agtagttgtt atcggcctgc ggacgcttgg
      841 ctcgcaagtc gctggcccgt cgagagttgc gacgaaaaag tgcgaagaaa ggcgcggttg
      901 cgcctgccca accagggggc taataattgg ttcaaagccg cgaccagtgg tcgcagtgcg
      961 catggtaaag cgcgaccact ggtcgcggct ttgagaggtt aggtcgcggg ccggccggag
     1021 acgtagacat cgccctccag ctcgcgaacc agcagctgct tgagcagcag cgcctgcgcg
     1081 actgctgcga tgccgccgcc accgaggggc gagcgagcta tcgcgccccc gatgagcttg
     1141 ggtgcgatga agacgtggac ttcgtcgatc tgcttggcat cgaagaacgc gccgaggact
     1201 tcgctgcccc cttcgacgag gatgttcgtc atgcggcggc ggccgagctc ggccaagagc
     1261 gcgtgcagcc gctcgacagg cgcggactgg ccgaggcgga gaacttcgca accggcggcg
     1321 gtcaggcggc tctcgtcgct atcggcggcg tccgggccgg cggcgatcag tgtgggatac
     1381 tggcgggcgg tgcggacgag ctggctgtcc gtgcggaggc tggcgtgcga atcgaggacg
     1441 atacgcgtgg agactcgcgg cggctgggcg ttttccaggc gggcggtcag aagcggatcg
     1501 tcgcgctcgg cggtgccgcg gccgacgacg atggcatcga cgcgggagcg caggcgatga
     1561 acgacggctc gcgaagcggg gctggaaatc cactggctgt cgccggtgcg cgtggcgagc
     1621 ttgccgtcga gcgtcatggc ccacttggcg atgacccatg gccggccggt cgccagcagc
     1681 ttgagatacg gcgcgttgag cgcgcgggct tcggcctcgt gcaaaccggt ttcgaccgtg
     1741 attcccgcgt cagccagctg cgccaagcca ccgccggcca cttgcggaaa tggatcgctc
     1801 atcgccgcga tcacgcggcg gatgcccgcc gcaatcaccg cctccgtgca gggcggtgtc
     1861 ttgccgtgat ggcagcaggg ctcgagcgtc acgtacagcg tcgcgccgcg ggcccgctcg
     1921 cccgccgccc gcagcgcctc gatttccgca tgcggcccgc cgaaccgctg atgccagcct
     1981 tcgccgacga cctgcccttc acccacgatc acgcagccca ccagcgggtt cggctcgaca
     2041 aagccttccc cacggcgggc gagcgtaatc gctcgctgca tcacttcaga atcgaaggac
     2101 gaagccatgt caaaatcaga atacagaatt cagaactcag aacgtcacac tctgaattct
     2161 gcactctgca ttttgaattc ctagtccatt cccggcagcc agagcttgct cttgccttcg
     2221 ctgccggcgg cggcgggcgc gccctggtcg ggagtccaga cggcggactt gggggcggga
     2281 gcggcagacg cgccggcggg catcgccgcg gcggctcccg gcaccgcgcc ggcgggcacc
     2341 gtcatcactg ggcggcccgt cgccggatcc attcgcacca ggcccagctg ggcgaggagg
     2401 ttgaacagcg cctgctgcac gccgggctcg cgggcgtgct tggtcgtgag caaattgaga
     2461 atccgccggc tctcctgctc ttcgccgcgc tgcaggcgat acggcaactc cgcgagcagg
     2521 aaccgggccg gcgacgcgcc cttggctttg aggatctcct gggctttttg caaatagctg
     2581 agggcctcgt cggcatccag ggccatgcgg gcgaggatgt cgtaggtctc cgccaggtcg
     2641 atgtcggtcc gcttcgcgaa gctcggccgg cggacgactt ccaggccgat gtgccgcacc
     2701 agccgcgggg cggcgacgat caccgcccgc cgccagacgc cgtgcagttg atcgtcggtg
     2761 agcttctcga ccgccaggcg cgtttgccgc gcgggcaaga gcgactcgac gcggacaccc
     2821 gacggatcga tcggctccgc ggccggcagg cccagcgagc ggcgaagctt gttgtggtcc
     2881 gggttctcga ccggctcggc caggtccatt tgcaggatga cggcctgcac acgaatttgg
     2941 ccgaagggat ccccaaccga ttgccggggc gaacgtccgt cgagcacgcc catcggcagg
     3001 ttcggccaga tgctcagcag cgtctgcgtg cgatgctcct ggatcatccg cttgcggaaa
     3061 tcgggcggcg tatcgtcggg gaatcgccag ttgatcgcca gcgcggcgct gaccgccggc
     3121 atgcgcccgg tttcctcttc gcccagcttt tcgccggcca acgggcccag cacgtcgcgc
     3181 aggtgcttga tccgattctc gaagtcggcg gtcttcaccg cggcgaactc cacgcgggcc
     3241 ggccggtcgg tttccttgcc gtacagcaac acctcgccca gcaccttggg cacgttgtcg
     3301 cgcgacaggc tggccgaatg ggccggcact tcgcgatcga gcagcaggaa cccaaacagc
     3361 ggcggcggct cgtcggcctc gcggaactgg gcggggtcga agggaatgct ctgcacccgc
     3421 ttgttcgaca gcagctgctc gcgcagcgcc tgagcgtcgg tcacggcaat cgtcaccgtg
     3481 atctccggca ccgtatcgac ttccgacagg tcgctgaggt actgcgccag cccctcggct
     3541 tcgaccgcat cgtcgcgcgg cacggccggc agcgccgcga atttacgcag cgcggccctc
     3601 gccccggcgt tgtcgatgac ccgcagcttg cagacggcca tgttccgcca gaccgccggc
     3661 tcctgcggga atttttgcgc cagggcctcg aacaatttgg ccgcggctcg ccagcagcca
     3721 agatcggcct gcttcagggc ctcgttgaac tcggcgattc ctgcagccga gagcgcgccg
     3781 tcggccggcg ccgagaccat cccggtcatg ccgtgcaccg ccagcgaaat ctgcccgccg
     3841 gattccagtt ccagcagatt catgaccgcg ttgcggtcct tgccttgcga ggctgccgcc
     3901 tggaacacgg cgtaggtctg ggccgccagc ggctcgccga cggcgttcag cgtccgggag
     3961 acgatgccca gcgcctcata caccaccggc agcagctgcc cctgctgatt ctcgagagcc
     4021 gtctggagct tatcgaccgc ctcttcggcc ttgccttcct ggcagtccag gatcgaggcg
     4081 atcgccaggc ccgcggcact cgtcggcatg accttgagca gccgcgccac tgtctcgcgg
     4141 caggtgtcga gctcgttcag cgccagctgc accgtcgcct tgtgcaccag gatcgccggc
     4201 tgatcgggct ttttctccag cagccggttg cacaggtcca gggccgccag ccgctgctcg
     4261 ccggccaggg cctcgtcgat tttggccagg tcggccagga cttccgggcc gcagcagaac
     4321 ttgttcttct tgccgctgcc gcaggggcac ggctgatagg gatcgagcga catggtttgc
     4381 cttagggatt catgaagaat ccccaagttt aaggccagcc cagcggggcg gatagaaggg
     4441 gcgggtgtgt ggtcctcacg ctccccgtga ggcgtccgct cgcggagcgc gcggactaca
     4501 ctacgtccac tgctgcacgt cgagcgtgag gttagcccgc tcttcgccgg cgatcgcttg
     4561 gatgtcgccc ttggcgacaa agtaagcctt gacgacctct ggatcccact gctgccccgc
     4621 gccgttcttg aagatctgct cgacgcgctc gaccggcatg ccggggcgat agggccggtc
     4681 gccggtcatc gcgtcgtagg cgtcggccac ggccacgatg cgggcgatga acggaatctg
     4741 gtcgccgacg agcttgaatg gatagccgcg gccgtcccac tgctcgtggt gatgcagcac
     4801 ggcgggcagc acgtcggcca gctgcttgat gtcagccaga atgcgatagc ccagctcggg
     4861 atgttgcttg atgtgctcga actcggccgg cgtcagcttg cccggcttgc ggagcacggc
     4921 gtcatcgatg ccgatcttgc cgatgtcgtg caacaggccc gccatgtaga gcgtgtgcag
     4981 catctcggct tcgcagttca gctcctgggc cagccgcacc gaaatgcggg ccacgcggtc
     5041 gctgtggccg caggtgtact ggtccttggc gtcgatcgcg ctggtcaggg cccgcaccac
     5101 gctggccaaa aactccgact gctgccggta caggtcgcgg ttggagctgt ggatgccgag
     5161 catcgccgcg accgaattca gcaggctggc ctcgatcgtg ccgaactcgc cgccgtccgt
     5221 gtgattgagc acggccagcc agccgaagac gcggtcgccc tcggtcatcg gcacgacgat
     5281 cagctcgcgc acgccagcaa accgccagct cgccgagccg gtgacactgc ggttggccac
     5341 gagcgggccg cagccggcct ccaggttcag gtcctcgatg agctgcgtga attgctggtt
     5401 gtcgagcggg cactgccccg cggtgagcag gacgctgcgg gtgcgggccc ggtaggtgat
     5461 ctgtccttcc ttggcgaccg gcagcagctg gatcgccacg gacttcgtcg ggatgcagtc
     5521 gagcagccac cgcagcacga gggcgcagag ctgctcttcg tcgctcctga tccgcaggtt
     5581 ctgcgtcacg ccgtgcagca ggcaaatctc ttcgtacgtc gccgccagat tgtccgagag
     5641 cttctcgacc tcgcgctcca gctgcaccgt tttctcttcg gctcgaacct gggcctgcac
     5701 ggccgttgcc aggcggagca gcgcctcggg cgaccagatc ggctgccgca gaatccaggc
     5761 cagggccttg ctctgatcga ggccgagcag gtgcgacggg ccagccaggt gctcgtgggc
     5821 ggcgacgggc cggaccacga acgccgccgt cgccacggtc gtgcggtccc gcgaaagcgg
     5881 cagcggcacg gccaacagca gcacgcagtc ttcgtccgag atgaactgcg gctggttgcc
     5941 gtgaaccgcc cgggccaatt gcccgcgaaa cggatcgctg ctcgccggct gctgcgtcga
     6001 agcgtgtagc aattcgcccg tctggccgtc ccagaacgtg aaggtcgcgc cgaacgcttc
     6061 ccgcagcgtc gccgaaatcc ggtcgatgac cggagtcgga tcgtcctccc acggctgcgc
     6121 ggcgacaaaa gattcgatat ccgcggtggc gatcatgatg acgccgcaca gtcgagtaac
     6181 accagcgccg caggggcggc gcagcctcag cgcaaaccta ggttgcccgg ccaaacaacg
     6241 tcggccccgg ccgcccccgg cgctaatgcg gacgcggcgg ccagcggttg tgcggcctgc
     6301 acgaccgata cagtcgatgc gatgcctata atcgaacgga actctgcctg cggagcctgc
     6361 gatggacagt ctgaccgccg cgcggctgca aatgacggtg tcgctggcgt tccacatggt
     6421 ctatgcggcg atcggcatcg gcctgccgct cctcttagta atcgtcgaag ggctctacct
     6481 gcggaccggg cagccgcact acctggcgct ggccaggcgg tgggcgaagg tgacgggcct
     6541 cctgttcgcg gtcggcgctg tgtcgggcac ggcgctctcg ctggagctcg gcctgctctg
     6601 gccgcgctac atggagctgc tcggtgcggt cgtcggtcac atcttcggcc tggagggcta
     6661 cgccttcttt ctcgaggcga tcttcatcgg cctctatctc tacggctggg agcggctgag
     6721 tccgctcgcc cactggtggt gcggcgtggt cattgcggcc agcggcatgc tctcgggcat
     6781 tttcgtgctc ggagtcaatg cctggatgca ggtgccggtc ggcttcgaac tggccggcgg
     6841 gcgcgtggtt gtcaccgacc cactcgcgat cttcaagcat ccgctctggg cgcacatggc
     6901 ctggcactcg accctcgcct gctacatctc cgtcgctttc gcggtcagcg gctggtacgc
     6961 ctggcggctg ctgcgctctc cgcacgacgc gtcgtttcgt gcggcattgc tcgtcgccat
     7021 gaccgtcggc tcgctggccg ccgtgctgca gccgctgtcg ggcgactacc tggccaagta
     7081 cgttttccgc acgcagccgg tgaagttcgc cgcgatggaa ggccagttcc agacgcaaac
     7141 gcacgcgccg ctgcggctcg gcggctggcc ggacgaagcg gcgggcaaga cccgctgggc
     7201 gctcgagatt cccggaggtt tgagctttct ggccgacggc aacccggcga ccgaagtccg
     7261 cggcctcgac cagacgccgc gggccgactg gcccaacgtc gggctgacgc acctggcgtt
     7321 ccaactgatg gtcggttccg gcatggccat ggtcctcgtg tcgattctgt tctggacgac
     7381 atatttctgg cgaaggcaag cattgttcga gagccgctgg ctgctctggc tcgtggtgct
     7441 ggccgggccg ttgggatttc tcggcctcga agccggctgg ttcgtgaccg aagtcgggcg
     7501 gcagccgtgg atcatccagg gcgtgctgcg gacgcgcgag gccgtgacgc cg
//
LOCUS       DASRJB010000352         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_381947, whole genome shotgun sequence.
ACCESSION   DASRJB010000352 DASRJB010000000
VERSION     DASRJB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381947"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="VFB80_09225"
     CDS             <1..1755
                     /locus_tag="VFB80_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33988.1"
                     /translation="EPAQAGDSAVTLAEPASGWRVGDRVILTATTLLRSGTYKREGGQ
                     TEERLIKAIDGVKLTLDQPLEYEHAADGDYRGEAANLSRNVVVESAEPEQSRGHTMYH
                     RGSAGSIRYAEFRHLGKPNALGRYSLHFHLCGDTMRGSSVVGASIWDSGNRWLTIHGT
                     NYLVVRDCVGYKSIGHGFFLEDGTEVNNVLDRNLAVQARAGKPLKGQVLPFDKNDGAG
                     FWWANCLNTFTRNVACDNEQYGYRFDATQTGRFDLKLSVLAPDGSRPKVDIRTLPFVR
                     FDDNECHSDGLYGMNLGEGVAKVGPDERHPLIVRNLRIWGTHYAFRGQSPSLLVENLK
                     IHRCRYGMYFPTFDQHVYRNLTISETPDEPFSAAYGDTSVQEGVVTIDGLAFENIGRG
                     NKILFPVTHYNASGRAVTHIRNVRMTGISKDRSLFNMYGGMWLKPPTDKGVAIFVHDY
                     FGPGKHAKVVSTQATDLLGDGSNYKELPPLTGEHSRVAEVQNVEFPTLLSPVDDRPPA
                     TVITDVLPAAGGKLLVRGTTSDNGAVKSVTVNGQLAVATAENFAQWEIRLDAPAAAKL
                     TALASDSAGNIERLAHQR"
     gene            1893..>2234
                     /locus_tag="VFB80_09230"
     CDS             1893..>2234
                     /locus_tag="VFB80_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN33989.1"
                     /translation="MYALPSSPRGPRRAFTLVELLVVIAIIGVLVALLLPAVQAAREA
                     ARRMSCGNNLKQLALGIHNYHDVHLVFPYEMYANPGWAWGAFILPFIEQQNLHQQLNP
                     GTPVALPQATTL"
BASE COUNT          414 a          753 c          714 g          353 t
ORIGIN      
        1 gagccggcgc aggccggcga ctcggccgtc acgctggccg agccggcgag cgggtggcgg
       61 gtcggcgacc gcgtcatcct caccgcgacc acgctgctcc gctcgggcac ctacaagcgc
      121 gaggggggcc agaccgagga gcggctgatc aaggcgattg acggagtgaa gctcacgctc
      181 gatcagccgc tggaatacga acatgccgcg gatggcgact atcgcgggga ggccgcaaac
      241 ctcagccgca acgtggtggt cgaatcggcc gagccggagc agtcgcgggg gcacacgatg
      301 tatcaccgcg gctcggcggg ctcgatccgc tacgcggagt tccgccacct gggcaagccg
      361 aatgcgctcg gccgctacag cctgcacttt catttgtgcg gcgacacgat gcggggcagt
      421 tcggtcgtcg gggcctcgat ctgggacagc ggcaaccgct ggctgacgat ccacggcacg
      481 aactacctcg tggtccgcga ctgcgtcgga tacaagagca tcggccacgg cttcttcctc
      541 gaggacggca ccgaggtgaa caacgtcctg gaccgcaatc tcgcagtcca ggcccgcgcg
      601 ggcaagccgc tgaaggggca ggtgctgccg ttcgacaaga acgacggggc gggcttctgg
      661 tgggccaatt gcctgaacac gtttacccgc aacgtcgcct gtgacaacga gcagtacggc
      721 tatcgcttcg acgccacgca gaccggccgc ttcgacctga agctgagcgt gctcgccccc
      781 gacggctcgc ggccgaaggt cgatatccgc acgctgccct tcgtgcggtt cgacgacaac
      841 gagtgccaca gcgacggcct gtatgggatg aacctgggcg agggcgtggc gaaggtcggc
      901 cccgacgagc ggcacccgct gatcgtccgc aacctgcgca tctggggcac gcactacgcc
      961 ttccgcgggc agtcgccgag cctgctggtg gagaacctga agattcaccg ctgccgctac
     1021 gggatgtact ttcccacctt cgaccagcac gtctatcgga acctgacgat cagcgaaacg
     1081 cccgacgagc cgttcagcgc agcctatggc gacacgagcg tgcaagaggg cgtcgtgacg
     1141 atcgacggcc tggccttcga gaacattggc cgcggcaaca agattctgtt tccggtcacg
     1201 cactacaacg ccagcggccg ggcggtcacg cacattcgca atgtgcgaat gacgggcatc
     1261 agcaaggacc gctcgctctt caacatgtac ggcgggatgt ggctgaagcc gccgacggac
     1321 aaaggcgtgg ccatcttcgt gcacgactat ttcggcccgg gcaagcacgc caaggtggtc
     1381 agtactcaag ccaccgactt gctgggcgac ggcagcaact ataaggagct tccgccgctg
     1441 acgggcgaac actcgcgcgt ggccgaagtg cagaacgtcg agtttccgac gctactaagt
     1501 cccgtcgatg accggccgcc ggctacggtg atcaccgacg tcttgcccgc tgccggcggc
     1561 aagctgctgg tgcgcggcac gacgagcgac aacggcgcgg tgaagagcgt caccgtcaac
     1621 ggccagctgg ccgtcgccac tgcggagaac ttcgcccaat gggagattcg gctcgacgcg
     1681 ccggccgcag ccaagctcac cgccctggcc agcgactccg ccgggaacat tgagcggctg
     1741 gcgcaccagc gatgattccc gccagcgact tggctgcgcc ttaaccgatc gtaacgcggc
     1801 acgatcggcg gcttctccgg gaaaacattg actggagttg atcattcgct agcatcagct
     1861 cgtagttgtc aaccgttctt gccggagccg atatgtacgc cctgccctcc tcgccgcgcg
     1921 gcccacggcg agccttcacc ctcgtcgagc tgctggtggt catcgccatc atcggcgtgc
     1981 tggtggcgct gctgttgccc gcggtgcagg ccgcgcggga agccgcccgc cgcatgtcgt
     2041 gcggcaacaa cctcaagcaa ctggccctcg gaattcacaa ctaccacgac gttcacctcg
     2101 tctttcccta cgagatgtac gccaaccccg gctgggcctg gggcgcgttc atcctgccgt
     2161 tcatcgaaca gcaaaatctc caccagcagc tcaatccagg aacgccggtc gcattgcctc
     2221 aggccacgac cctg
//
LOCUS       DASRJB010000353         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_662237, whole genome shotgun sequence.
ACCESSION   DASRJB010000353 DASRJB010000000
VERSION     DASRJB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662237"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFB80_09235"
     CDS             complement(<1..203)
                     /locus_tag="VFB80_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN33990.1"
                     /translation="MPGSLARLLADYVPLWHRLMRLPGQLFAISPFDGDSRPSRVRLF
                     QPGSGRPTLPFPHPWNYDQPIRIL"
     gene            complement(332..1585)
                     /locus_tag="VFB80_09240"
     CDS             complement(332..1585)
                     /locus_tag="VFB80_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZN33991.1"
                     /translation="MKLSPSVVDHCRRQFPALSRRVGERTAAFFDGPGGTQVPQRVIE
                     AVSHYLAHTNANYGGLFATSRESNKLLAEAERGLADFLGADDPETIVFGQNMTSLTFA
                     LSRSLGRTWKSSDEIIVTRLDHDANVSPWVLAARDAGATVRFANIRAEDCTLDLEELR
                     HLINERTKLVAVGSASNATGTISPVRDVADWAHQVGAVVFVDAVHFAPHGLIDVQALG
                     CDFLACSAYKFFGPHVGVLWGRKSLLEKLEAYKVRPAPLAPPHKWMTGTQSHEAIAGA
                     LAAVEYLADLGRTLAGSDALPRRAALREAYREIGLYERGLCARLLAGLDQLPEIQVFG
                     INSPERMEERVATISFTHDRFSAEQMAEQLGRAGFFTWHGNYYALNLTETLGLEPAGM
                     LRVGLLHYNTLTEVERFLEALHGLR"
     gene            complement(1645..2013)
                     /locus_tag="VFB80_09245"
     CDS             complement(1645..2013)
                     /locus_tag="VFB80_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZN33992.1"
                     /translation="MQPAERVRVYDLEERSYLFAKRVRALVKRMPRTMSNFEDGKQVV
                     RSSGSIGSNYIEANEPLGEKDFLVHMKISRKESKESRYWLRLLDLDGNEELEVERRDL
                     IQEAHELMHIFNAIIRKRSA"
     gene            complement(2086..3369)
                     /locus_tag="VFB80_09250"
     CDS             complement(2086..3369)
                     /locus_tag="VFB80_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN33993.1"
                     /translation="MLRATPDDRRRQQQMPREGLAQHQLFRLRKLLTEIVPHNRFYSE
                     KLGLVDCDLASLADLERLPFTFKDELVTAPSADEFAANLTYPLERYVRYHHTSGTRGR
                     PLPVLDTAEDWQWWIELWQFVLDQAEVGPEDRALLAFSFGPFIGFWSAHDATVARGTM
                     VIPSGGMNTLSRVELARRMRATVLFCTPSYALRLAEIALENRVDPASLDVRRIVVAGE
                     PGGSVPALRRRIESLWNARLIDHAGASEIGPWGVGDREGRGLFVIETEFIAEFRSLAT
                     GGPADDGEISELILTTLGRYGSPVIRYRTGDVVRPRFAAEGFVLLEGGVLGRADDMLI
                     VRGVNIFPSSVEQILRAFPEVVEFRVTAQRQGSLDSLLVEIEDRLNNPDRIARELQLR
                     LGLKVEVRPAPIGSLPRFEGKGKRFHDQRAANYNI"
     gene            complement(3389..4213)
                     /locus_tag="VFB80_09255"
     CDS             complement(3389..4213)
                     /locus_tag="VFB80_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33994.1"
                     /translation="MSELPYPDRVPDPNNEELPADPWNPLVVSRGGSPVAVRWSQGLW
                     LHAVALAGNDQHCSILLADGRTTQLPRASVVAIPTRPVFQVGHEVMARWRNAAMFPGT
                     LTSIGDMGHTVAWHDGDVPMPVPLGSLTFLEWCQTTEPAVGPPPGSAAKLELKPPVVP
                     VPEIVPGAWVAIRGRNGYWIASVEAAVDGGFNVRFTDGARATVPDSDVFPMPEGNVFE
                     VGDEVLALWKGGSLYPGTITEESHEGYTVAWHDGDMPLVVKPGTLTYLFWVLEGTQ"
     gene            complement(4292..5083)
                     /locus_tag="VFB80_09260"
     CDS             complement(4292..5083)
                     /locus_tag="VFB80_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZN33995.1"
                     /translation="MPRRLGPLALLLVMSATTLAADNRDRFEARTFEGGDLQLPYRLL
                     MPKDYDPKQKYPLVVFLHGAGERGTDNKVQLVHGMNDFASDEVMAKYPAFVVAPQCPS
                     DQRWVEVDWTLDKHTQPKEPGAALAATLKLVDALQRELSIDGRRIYVTGLSMGGYGVW
                     DALARRPELFAAAAPICGGADLATAGKIKAIPIWAFHGERDTVVKPHRSQGMIAALKA
                     AGAAQPAAGNTAPKYTEYPGVGHDSWTPTYRDPAFYEWLFAQRKP"
     gene            complement(5177..5452)
                     /locus_tag="VFB80_09265"
     CDS             complement(5177..5452)
                     /locus_tag="VFB80_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN33996.1"
                     /translation="MLPLDPGLLFWLLAALHLAGLASMLLSRLPQSHRMQSVSQRVFL
                     LCLVFVGLATFFTICVQSNCWVWSGTTFSLMAVGATADLGQAARAGF"
BASE COUNT          951 a         1790 c         1893 g          926 t
ORIGIN      
        1 agaatgcgga tcggctggtc gtagttccag ggatgcggaa agggcagcgt cggccggccc
       61 gaaccgggct gaaagagccg tacgcggctg ggccggctgt cgccgtcaaa cgggctgatt
      121 gcaaacagct gccccggcag gcgcattaac cggtgccaga gcgggacata atcggccaat
      181 aatcgcgcga gacttccggg catggcgtcg ggagtcgggg agtcggtcgg tcggtggtcg
      241 gtttccatgc tgagtactca gtacgaatac cacgcaccac cactcatcac tgacctaccg
      301 actatcggct accgacggac cgaccactgg cctaccgcaa tccgtggagg gcttcgagga
      361 accgctcgac ttcggtgagc gtgttgtagt ggagcaggcc gacgcggagc atgccggcgg
      421 gttccagccc gagcgtctcg gtcaggttca gggcgtagta gttcccgtgc caggtgaaga
      481 agccggcgcg gccgagctgc tcggccatct gctcggcgga gaagcggtcg tgcgtgaagc
      541 tgatggtggc gacgcgttct tccatccgct ccggcgagtt gatgccgaag acctgaatct
      601 ccgggagctg atcgaggccg gccagcaggc gggcgcagag gccgcgctcg tagaggccga
      661 tctcgcggta ggcctcgcgg agagcggccc gccgcggcag ggcgtcgctg ccggcgagcg
      721 tgcggccgag gtccgccagg tactcgaccg cggccaatgc gccggcgatc gcctcgtggc
      781 tttgtgtgcc ggtcatccac ttgtgcggcg gggccagcgg cgcggggcgg accttgtagg
      841 cttccagttt ttccaagagc gacttgcggc cccagaggac gccgacgtgc ggtccgaaga
      901 acttgtaggc cgagcaggcc aggaaatcgc agcccagggc ctgcacgtcg atcaggccgt
      961 gcggggcgaa atggaccgca tcgacgaaca ccaccgcgcc gacctggtga gcccaatcgg
     1021 cgacgtcgcg cacggggcta atcgtgccgg tggcgttgct ggcggagccc acggcgacga
     1081 gcttggtccg ctcgttgatc aaatgccgca gctcttccag gtccagcgtg cagtcctcgg
     1141 cccggatatt ggcaaaccgc acggtcgcgc cggcgtcgcg ggccgcgagc acccaggggc
     1201 tgacgttggc gtcgtggtcc agtcgcgtga cgatgatctc gtcgctgctc ttccaggtgc
     1261 ggccgagcga ccgcgagagg gcgaaggtga gcgaggtcat gttctggccg aagacgatcg
     1321 tttcgggatc atccgcgccc agaaagtcag ccaggccgcg ctcggcttcg gccaagagct
     1381 tgttgctttc gcgcgaggtg gcaaagaggc caccgtaatt cgcgttggtg tgggccaggt
     1441 agtgactgac cgcctcgatc acccgctgcg gaacctgggt cccgcccggc ccatcgaaga
     1501 acgccgccgt ccgctccccc acgcggcgcg acaacgccgg aaactggcgg cggcaatgat
     1561 ccacaacgga gggcgagagt ttcatgggcg tcgaaaaatc gaagaaccaa gaaccgaaga
     1621 accaaagaac caaagggagg caggtcaggc tgaccgcttg cgaatgatgg cgttgaaaat
     1681 gtgcatgagc tcgtgggcct cttgaatgag atcccggcgt tccacctcca actcttcatt
     1741 cccatcgagg tcaaggagtc tcagccaata ccgagactcc ttcgactcct tacgggatat
     1801 cttcatgtga accagaaagt ccttctcgcc aagaggttcg ttggcctcga tatagttgga
     1861 accgatggag ccagacgacc gaacgacctg cttgccgtct tcgaagttgc tcattgtcct
     1921 tggcatccgc tttaccaagg cccgaacgcg cttcgcaaac aggtagctgc gttcctccaa
     1981 atcatagacc ctgactctct ccgcaggctg cattgctgct ccttcagttc ttcagttctt
     2041 cggttctttg gttcttcggt tctttggttc tttgatattt gtccttcaga tgttgtaatt
     2101 cgccgcccgc tgatcgtgga atcgcttgcc tttgccttcg aaccgcggca gcgagccgat
     2161 cggggcgggg cggacttcga ccttgaggcc caggcggagt tgcagctcgc gggcgatgcg
     2221 atcggggttg ttgagccggt cctcgatctc gacgagcagc gaatcgagac tgccctgccg
     2281 ctgggcggtg acgcggaact cgacaacctc gggaaaggcc cgcaggattt gctcgaccga
     2341 gctggggaag atgttcacgc cgcggacgat cagcatgtcg tcggcccggc cgagcacgcc
     2401 cccttccagc agcacgaagc cttcggcggc aaaccgcggg cggacgacgt cgccggtgcg
     2461 gtagcgaatg accgggctgc cgtagcggcc gagcgtggtc aggatcagct ccgagatttc
     2521 gccgtcgtcg gccggcccgc cggtggccag cgagcggaac tccgcgatga attccgtttc
     2581 gatgacgaac aggccgcggc cctcgcggtc gcctacgccc caggggccga tctcggacgc
     2641 gccggcgtga tcgatcagcc gggcattcca gagcgattcg atccgccgcc gcagggccgg
     2701 caccgagccg ccgggctcgc cggcgacgac gatccgccgc acgtcgaggc tggcgggatc
     2761 gacgcggttt tccagggcga tttcggccag ccgcagggcg tagctgggcg tgcaaaacag
     2821 cacggtggcc cgcatgcggc gggccagctc gacgcgggag agcgtgttca tgccgccgct
     2881 gggaatcacc atcgtgccgc gagccacggt cgcgtcgtgg gcgctccaga aaccgatgaa
     2941 cgggccgaac gaaaacgcga gcagtgcgcg gtcttcgggg ccgacttcgg cctggtcgag
     3001 gacgaactgc cagagctcga tccaccactg ccagtcttcg gcggtgtcga gcacgggcag
     3061 cggccggccg cgcgtgccgg aagtgtggtg atagcggacg tagcgctcca gcggatacgt
     3121 cagatttgcc gcgaactcat cggcggacgg cgcggtgacc agctcatcct tgaacgtgaa
     3181 cggcagccgc tccaggtcgg cgagcgacgc caggtcgcag tccaccaggc ccagtttctc
     3241 cgaatagaac cggttgtgcg gcacgatctc cgtcagcagc ttgcggagcc ggaagagctg
     3301 gtgctgcgcg aggccctcgc gcggcatctg ctgctggcgg cggcgatcgt caggcgtggc
     3361 gcggagcatg gcggtcgtcg gggattggct actgcgtacc ctccagcacc cagaacaaat
     3421 acgtcagcgt accgggcttc accaccaggg gcatgtcgcc gtcgtgccag gcgacggtgt
     3481 agccctcgtg cgactcttcc gtgatcgtgc cgggatagag gctgccccct ttccacaggg
     3541 cgagcacctc gtcgccgact tcgaacacgt tgccctcggg catgggaaag acgtcgctgt
     3601 cgggcacggt ggcccgggct ccgtcggtga agcggacgtt gaatccgccg tccaccgcgg
     3661 cctcgacgct ggcgatccag tagccgttgc ggccgcggat ggcgacccac gcgccgggca
     3721 cgatttccgg aacaggcacg accggcggct tgagttctag tttcgcggcg cttccaggcg
     3781 gcggtccgac cgcgggctcg gtcgtctggc accactcgag gaacgtcagc gagccaagcg
     3841 gcacaggcat gggtacgtcg ccgtcgtgcc aggcgaccgt gtggcccatg tcgccgatcg
     3901 acgtgagcgt gccgggaaac atggccgcat tccgccagcg agccattacc tcgtggccga
     3961 cctggaacac gggccgcgtg ggaatcgcga cgacgctggc ccgcggcagc tgcgtggtcc
     4021 gcccgtcggc cagcaggatc gagcaatgct ggtcgttgcc tgctagcgcg acagcgtgca
     4081 gccacaggcc ctgcgaccag cgcacggcca ccggcgagcc gccgcgcgac accaccagcg
     4141 gattccacgg gtcggcgggc agctcctcgt tgttcggatc aggcacccgg tcgggatagg
     4201 gcaattcgga catgcgaggg ccagggttcg ggggttccgg gttccgatcg tcaattagac
     4261 cataatcacc gatgctgaac cttgccccac cttacggctt tcgctgggcg aacagccact
     4321 cgtagaaagc cggatcgcga taggtcggcg tccaactgtc gtggccgacg ccgggatact
     4381 cggtgtattt cggcgctgta tttccggcgg cgggctgcgc tgcccccgcg gccttcaggg
     4441 cggcgatcat gccctgcgag cggtgcggct tgacgacggt gtcccgctcg ccgtgaaacg
     4501 cccagatcgg aatcgccttg atcttgcccg ccgtggccag atccgcgccg ccgcagatcg
     4561 gtgcggctgc cgcgaacagc tccggccgcc tggcgagagc gtcccagacg ccatagccgc
     4621 ccatcgacag tcccgtgacg taaatccgcc gcccgtcgat agagagttcc cgctggagcg
     4681 cgtccacgag tttcagcgtc gcggccagcg cggcgccggg ctctttgggc tgggtgtgct
     4741 tgtcgagcgt ccaatcgact tccacccacc gctggtcgct ggggcactgc ggcgcgacca
     4801 cgaaggccgg atattttgcc atgacctcgt ccgacgcgaa atcgttcatg ccgtgcacga
     4861 gctgcacctt gttgtccgtg ccgcgctcgc ccgctccgtg cagaaagaca acgagcgggt
     4921 atttctgctt tgggtcgtag tccttcggca tcagcagccg gtagggcagc tggagatcgc
     4981 cgccctcaaa cgtgcgggct tcgaagcggt cgcggttatc ggcggccaac gtggtggcag
     5041 acatgacgag gagcagggcc agcggcccaa ggcggcgagg catgcggcgg attccgagcg
     5101 aggaattcgg attacagcgt atggtactgt ccgcgagcct cgcccggcgc tcgccccgca
     5161 agccggcgcg gcgaatctag aagccggccc gcgccgcctg gcccaaatcg gcggtcgccc
     5221 cgacggccat cagcgagaaa gtcgtgccgc tccagaccca gcagttgctc tgcacgcaga
     5281 tcgtgaagaa tgtcgccagg ccgacaaaca ccaggcacaa caggaacacg cgctgcgaca
     5341 ccgactgcat gcggtgcgac tgcggcagcc gcgagaggag catgctcgcc aggcccgcca
     5401 ggtgcagcgc ggccaatagc caaaacaaga gccctgggtc gaggggcagc atgcggaact
     5461 ccgattccgt tcgggggctg ccgggcagcg gattgcgggc cgctttccgg caaatcgtcg
     5521 tgcttcagcc catccttgag ctgcgccgcg ccgccggggg
//
LOCUS       DASRJB010000354         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2356420, whole genome shotgun sequence.
ACCESSION   DASRJB010000354 DASRJB010000000
VERSION     DASRJB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356420"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFB80_09270"
     CDS             complement(<1..895)
                     /locus_tag="VFB80_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HZN33997.1"
                     /translation="MGQMRFVIPRPERLVGAAAEQAYLAGAEGIPWECQTTLEGIELI
                     LERDTRESGYLYFPWKVTGRGLLQLCSGSLMERPNAYHLPVELARGTLNRVRNQAGQW
                     EQAGMTIPPAFEEMIHAAALIFARAATGQDHPEAAQDFAEEAIRLGLDAGDLLSREYT
                     QQVLAIRRGQQASLGTLLGARLQAVPTGEAATRFQSAFNAAAIGTFWPDLEPQLGRFK
                     WEATDKLVEWCRQRELRLCMGPLVHMDKHALPDWLFLDEDFEEVQTSVLAFIDEVVKR
                     YRGKVDLWHVAAGMNLDGALEL"
     gene            1037..1924
                     /locus_tag="VFB80_09275"
     CDS             1037..1924
                     /locus_tag="VFB80_09275"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZN33998.1"
                     /translation="MSALVESRLPGVPVRRGKVRDVYDLADRLLLVATDRISAFDWVM
                     PQGILDKGRILTQLSSYWFELLRVPNHLISTEVASLELPPGSTSREQLAGRSMLVHKC
                     EVVPVECVVRGYLEGSGWKEYRQNQAVCGVPLPPGLAQCSQLPQPIFTPATKEESGHD
                     QNISLERMSQIVGADVAQELRRRSLEVYSRAAAHAGERGIIIADTKFEWGWAGGELIL
                     IDEVLTPDSSRFWPADRYQPGRAQPSFDKQFLREWLETTSWDKNSPPPNLPDDVVSRT
                     REKYVEAYERLTGNRFAAT"
     gene            complement(1952..>2785)
                     /locus_tag="VFB80_09280"
     CDS             complement(1952..>2785)
                     /locus_tag="VFB80_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN33999.1"
                     /translation="HISWAGPNRPLPREINPRHVYERLFRASRPQTAAAEQDKLLLDR
                     VLGDARKLREQLGTADRQRLDEYLEGVRSLEERLDRSVAGFNSDWQPRADVGANKLPE
                     QDPATHDDNVRWMLDMLALAFQSDTTRIATFMFGNAVSGRNFSFLEDVRGGHHDISHH
                     ENDAEKLRMYQRINQWHIEQYAYLLGKLKGMKEGEGTVLDNCLILFGAGLRDGNSHSP
                     HNLPLVLAGRGGGRLASGQHLVYERDSPLSNLYVSMLDAFGTPVERFADSTGPLAGVL
                     A"
BASE COUNT          460 a          941 c          923 g          461 t
ORIGIN      
        1 tcagctccaa tgcgccgtcg aggttcatcc ccgccgcgac gtgccacaaa tcaaccttgc
       61 cgcgatagcg cttaacgacc tcgtcgatga acgccagcac ggaggtctgc acttcctcga
      121 aatcctcgtc gaggaacagc cagtccggca gcgcgtgctt gtccatgtgc accagcgggc
      181 ccatacagag ccgcaattcc cgctggcgac accactcgac cagcttgtcg gtggcctccc
      241 acttgaatcg gcccagctgc ggctcgaggt cgggccagaa cgtgccgatc gccgcggcgt
      301 tgaacgcgct ttgaaaccgc gtcgccgcct cgccggtggg caccgcttgc aggcgggcgc
      361 ccagcagcgt gcccagcgag gcctgctggc cgcggcggat cgccagcacc tgctgcgtgt
      421 attcgcggct gagcaggtct ccggcgtcca ggcccaggcg gattgcctct tcggcgaagt
      481 cctgcgccgc ttcgggatgg tcctgccccg tcgccgcgcg ggcaaagatc agcgccgcgg
      541 cgtggatcat ctcctcgaac gcgggcggga tggtcatccc ggcttgctcc cactggccgg
      601 cctggttccg cacgcggttg agcgtgccgc gggccagctc gaccggcagg tgataggcgt
      661 tcggccgctc catcaggctg cccgagcaga gctgcaacag gccgcggccg gtcaccttcc
      721 agggaaaata gaggtagccc gactcgcgag tgtcccgctc caggatcagc tcgatgccct
      781 cgagcgtggt ctgacattcc caggggatgc cttcagcgcc ggccagatag gcttgctcgg
      841 ccgcggcgcc caccagccgc tccggccggg gaatcacgaa acgcatctgc cccatgaaat
      901 cccttcccgc gtccgcccct ttctcgaaca cgaatacgca gcgaaaagtg atgcagggga
      961 gtctaatcca cccgccctgc ggcggcaacc aaatccgcca ctggccgcgt gccggcggcg
     1021 ctatataact gcgggcatga gtgcgctcgt cgaatcccgc ttgcccggag tgcctgtccg
     1081 ccgcggcaag gtccgcgacg tgtacgacct cgcggaccgg ctgctgctcg tcgcgaccga
     1141 ccggatcagt gcgttcgact gggtgatgcc gcagggcatt ctcgacaagg gccgcatcct
     1201 gacgcagctc agcagctact ggttcgagct gctgcgcgtg ccgaaccact tgatttcaac
     1261 cgaagtggcg tcgctggagc tgccgccggg gagcaccagc cgcgagcagc tggccgggcg
     1321 gagcatgctc gttcacaagt gcgaggtcgt gccggtggaa tgcgtcgtcc gcggctacct
     1381 ggaaggctcg gggtggaagg agtaccggca gaaccaggcg gtgtgcggcg tgccgctgcc
     1441 gccgggcctg gcccagtgct cgcagctgcc ccagccgatc ttcacgccgg cgaccaagga
     1501 ggaaagcggc cacgaccaga atatttcgct ggagcggatg agccagattg ttggggccga
     1561 tgtcgcgcag gaattgcggc ggcgaagcct ggaggtctat tcgcgggccg cagcccacgc
     1621 cggggagcgc ggcatcatca tcgcggacac gaaattcgaa tggggctggg ccggcggcga
     1681 gctgatcctg atcgacgaag tgctcacgcc cgacagctcg cgattctggc cggccgaccg
     1741 ctatcaaccc gggcgggcgc agccgtcgtt cgacaagcag ttcctccgcg aatggctgga
     1801 aacgaccagc tgggacaaga atagcccacc gccgaatttg ccggacgacg tcgtctcccg
     1861 cacgcgcgaa aagtacgtcg aagcgtacga acgcctgacg ggcaatcggt ttgcggccac
     1921 ttaagtccac tctcccttga ggagagggag tctaggccag cacgccggcc agcgggccgg
     1981 tgctgtcggc aaaacgctcg acgggcgtgc cgaaggcgtc cagcatcgag acgtacaggt
     2041 tcgacagcgg cgagtcgcgc tcgtagacca ggtgctgccc gctggcgagc cgcccgccgc
     2101 cgcggccggc caaaaccagc ggcaggttgt gcggactgtg gctgttgccg tcgcgcaggc
     2161 cggccccgaa caggatcagg cagttgtcca ggacggtccc ctcgccttcc ttcattccct
     2221 tcaacttccc tagcaggtag gcgtactgct cgatgtgcca ctggttgatc cgctgataca
     2281 tccgcagctt ctcggcgtcg ttctcgtggt gcgagatgtc gtggtgcccg ccgcggacat
     2341 cttcgagaaa cgagaagttt cgcccgctga ccgcgttgcc gaacatgaac gtcgcgatcc
     2401 gcgtcgtgtc cgactggaag gccagcgcca gcatgtccag catccatcgc acgttgtcgt
     2461 cgtgcgtggc cgggtcctgc tccggcagct tgttggcccc aacatcggcc cgcggctgcc
     2521 agtcgctgtt gaaacccgcg acggagcgat ccagccgctc ttcgagcgag cggacgcctt
     2581 cgagatattc gtcgagccgc tggcgatcgg cggtccccag ctgctcgcgc agttttctgg
     2641 catcgcccag cacgcggtcc agcagcagct tgtcctgctc ggcagccgcg gtctgcggcc
     2701 ggctagcgcg gaacagccgc tcatagacgt ggcggggatt gatctcgcgg gggagcgggc
     2761 ggttggggcc ggcccaggaa atgtg
//
LOCUS       DASRJB010000355        12606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2066593, whole genome shotgun sequence.
ACCESSION   DASRJB010000355 DASRJB010000000
VERSION     DASRJB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066593"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFB80_09285"
     CDS             <1..716
                     /locus_tag="VFB80_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN34000.1"
                     /translation="AKKYPVIEKIYAGPQGSFTPKSFSTQGLNSTLTDLGFVVVQMDG
                     MGTANRSKAFHDVCWHNLGDAGLPDHILWHKAVAAKYPWYDISRVGIYGGSAGGQNST
                     GAMLFQPDFYKAAVSGCGCHDNRMDKASWNEQWMGFPVGPQYGKSSNIDNAGKLRGKL
                     MLIVGEMDTNVPTESTFRLADALIKKGKDFDLVVVPGAGHGMGGSYGTRRMNDFFVRH
                     LLGIEPPDRNAAPSGPSGE"
     gene            726..1115
                     /locus_tag="VFB80_09290"
     CDS             726..1115
                     /locus_tag="VFB80_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34001.1"
                     /translation="MDWRKSPLRWIAALAAALTVIQPAWLLIEFARGRGPLPLVLLVF
                     NYALVVVITLWITLDARERRRTPCFDYGFFLLILWPFSLFWYCGCTRGWRGIGLACVL
                     YVLAWLPAFTTAVIIMIAAIAAAVLNP"
     gene            1129..3228
                     /locus_tag="VFB80_09295"
     CDS             1129..3228
                     /locus_tag="VFB80_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN34002.1"
                     /translation="MSGRRFTLILFAAAALASQTSARGQQEIVAETPKNLTPIDRTPI
                     VEAPITDGDRAHWAFAPVARQSLPQAKIPAWPRTAVDAFILAKLEAEGIAPSAEASRA
                     TLLRRLSFDLTGLPPTPQELAAFQADASPDAYERQVDRLLASPAFGERYGQYWLDLAR
                     FAETDGYEHDKVRAQAWKYRDWVIAAINADLPYDEFIRQQIAGDEVAGSKFKVQGSKS
                     EPTTLNFEPGTLNSSAASMFCLSGPDMPDINDQLERRHSLMNELTATVGSAFLGLQLG
                     CAQCHDHKYDPLSQADFYRLRAVFEPAVPALKRDVPISALASQKETESARLWVRGDHR
                     RPGLEVQPSFPRIASAPTGRAGEGVHEESDTPSLARRASVRSELADWLVRADNPLTSR
                     VMANRVWQGHFGRGICQTPSDFGVMGGPVTHRELLDWLAAELRESGWGLKGLHRKIVT
                     AATYREISDFRFPISDLEAKGSANLRSEILNLKSELYGRYPRRRLEGEAIRDAMLAAA
                     GLLTSERGGPGVLPPLPEELVGTLLKGQWTASQREADHYKRSVYVFARRNLRYPIFEA
                     FDRPDGNASCPIRNKSTTAPQSLLLFNSEFSLLAARRLAGRVMNIEEPKNQRTEEQIE
                     RLYEIALSRQPTAAEITTFSKFLADQQQRLKAEARPVKELALPVPCPDTADADAAAAL
                     VDACLAVLNANEFIYVD"
     gene            complement(3340..3909)
                     /locus_tag="VFB80_09300"
     CDS             complement(3340..3909)
                     /locus_tag="VFB80_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN34003.1"
                     /translation="MDRFWLLTWTTYGTWLPGDKRGFVSEVNDGQGGRVIHNIPQAPY
                     DADWEHLAHDARNRLKCAPILLTSQQAQTVLSQLHETANYRQWQLIAAATMANHVHVV
                     VGVPGDPPPEAILRDLKSYASRMLNRGFQRPASGTWWTESGSKRKLKNEAAVLGAVAY
                     VRDQEYPLANWIHPGFLAELGDGIAPGKQ"
     gene            complement(3935..5176)
                     /locus_tag="VFB80_09305"
     CDS             complement(3935..5176)
                     /locus_tag="VFB80_09305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZN34004.1"
                     /translation="MTDQTSQGRRIGILTAGGDCPGLNAVIRGVVKSANTHGYDVIGF
                     LKGYEGLVDPVSFIPLTHKNTAGILGQGGTILGSTNKGRFAATIGVNDRLTLDPELIA
                     GVKTTIEQLSLDGLICVGGDGSLAVAQQFHEQGIPVVGVPKTIDNDLSSTAFTFGFFS
                     AVFCGTDALDRLHTTAASHERVMVLEVMGRHAGWIALYAGIAGGGDVILIPEIPWTFE
                     NVCQAIIGREQRGKRFTLVVVAEGAKLPDGGLVTQDKQECQKQVRLGGIGTFVGREIE
                     RRLGKETRTVVLGHLQRGGAPTTFDRVLATQFGAHAVRLIVERKFGHMVTYQPPDLND
                     VAIADAIKLSSVCPNCSAVQAARALGVCFGDDYRELPFKCESGQEVRGAEQNGRRAGG
                     VSLPMAVRRPAGSRRKRERDS"
     gene            5520..6575
                     /locus_tag="VFB80_09310"
     CDS             5520..6575
                     /locus_tag="VFB80_09310"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZN34005.1"
                     /translation="MSILRVAAADELKETAVFTAGAEGYHTYRIPALLVTKGGTVLAI
                     CEGRKTGTGDHGDLDLVQKRSTDGGKTWGPLELIHEEGGTAKVTIGNPCPVVDQSTGV
                     IWLPFTRDNDGVFVMSSKDEGKTWSQPREITADVKPKEWNWYATGPGNGIQLKSGKYA
                     GRLVIPCDHRVGTATKDDKTGWDKAGRSHVIYSDDHGATWKLGGATDYSMNECAVIER
                     ADGSLLLNSRSYRGKACRGVSISKDGGATWEPTTDDPALVESVCQASMIRAEGAAVLF
                     SNPATPRGRHHLTVRLSLDDGQTWPHSRLICEGPSAYSSLAVLPGGEIGLLYEQGPYK
                     SLTLARFPLAWVKTAQN"
     gene            6761..8506
                     /locus_tag="VFB80_09315"
     CDS             6761..8506
                     /locus_tag="VFB80_09315"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN34006.1"
                     /translation="MTLALLLGSIAAAQTDRSPVDLVLGPDETWLATVNQTADSVSLV
                     RVSDGKVLDEAAVGDHPIGIALSANGKTLLVSCHHSNEVALLEVRGEKLAKAGAIDVG
                     YQPHGIAVAEGGRTAYVACTASAEVAVLDLQQKQVASRIAVGRWPRNLALSRDGRRLI
                     VGTSGDRGVTLVDCTERKIVQQEQFVGLNIGHFAVTKDGRHAYFPWMVYRNNPITPGN
                     IRLGWVLASRIARVRLDEASRREAMSLDPQGKAIADVEGIALTSDDARLLVTASGTHE
                     LLVYKTEGLPLKDYGGSDHIDPALLKDDERFHRIELGGRPMGLAVAKDDRTVYIANYL
                     DNCVQVVDLADRKVIRTLPLGPGAEPSLARRGEAIFYDARRSLDQWYSCHTCHYEGGT
                     NSIPTDTTNDGTAFTFKTVLPLHNLRETGPWTWHGWQTDLAAAMKKSLTETMLGPPPA
                     EGDADALLAYLDSLKLPPNPFVGKDGALSASAERGKLLFAGDKAACASCHSGSYFTDG
                     QVHDVGLGSARDRYKGYNTPSLLGLYRRVKLLHDGRVDSLEQLLAGPHAPEKVSGSKL
                     SDDELRDLIEYLKTL"
     gene            8568..10310
                     /locus_tag="VFB80_09320"
     CDS             8568..10310
                     /locus_tag="VFB80_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN34007.1"
                     /translation="MHDIPLILTVTYGLAFALLFGTITNRFGLSPIVGYLLAGFMVGP
                     KTPGFVADPKIAAQLAEFGVILLMFGVGLEFHWRDLLQVKSLAIPGALFQSAVATALC
                     TAMAPLFGMSLASGVVLGIAVSVASTVVLVRLLMDNGVLHTPEGHVAVGWLVVEDLFT
                     VVVLVLLPALNAALAEGGAAAIAGSIAWTAAKIVLLVLLVLVIGARAVPLLLNYMART
                     RSRELFTLSVLAVALGIATGSALVFGVSMALGAFLAGMAVGQSDVSQQAASDALPMRD
                     AFAVLFFVSVGMLFDPASLAEHWPLALAVTAVILIGKPLAALAIVLVLGYPIRTGLTV
                     AVALAQIGEFSFILSEQARSLGFLSQAASSVLVTCSIISITLNPLLFKLVEPAERWLK
                     GRPGLLRRLARRAKSNPLAEIAALETHGKVAQAIVVGYGPVGQTLTAILRDFHVSPVI
                     IDLNVDTVRRLRHEGWQAIYGDARSTEILRNAQIEKVRYLLLTLPDEAARLAIIATAL
                     SLNPELKIIVRARYLAERDVLREADVDAVAFEEAEVAAALGEMLLKEMGADEDEVLAR
                     SAKIRADLSVGGKP"
     gene            complement(10321..10854)
                     /locus_tag="VFB80_09325"
     CDS             complement(10321..10854)
                     /locus_tag="VFB80_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZN34008.1"
                     /translation="MRSIATILILVLLLGFLIAGCGEKPRSVAELDASELLALIQDQQ
                     HLDPRNHVETDLGRFRITHPLAAGEGQMHVQFQLVAILPQARQERLAELLPPVEKRVR
                     DAVISLVQRSETEYLTDPGLEYFKAEVVRAINRVLQEPLVVDVAFSDFSMDRDDGMPW
                     SVPAPERESKPAGGGQH"
     gene            complement(10945..11652)
                     /locus_tag="VFB80_09330"
     CDS             complement(10945..11652)
                     /locus_tag="VFB80_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZN34009.1"
                     /translation="MCGRYTLRTPLHVLAQQFLFELGDLPADLELKPRFNIAPTQTVA
                     GIRRLEAGGARQLALFHWGLIPSWAKDAKLASSTINARGETLAEKPAFRTAFSRRRCL
                     VLADGYFEWKAVGKKKQPLLFQLKSGQPFAFAGLWERWRGPEGTPPDSPPLESCTIVT
                     TSANTLSANVHDRMPVILDPADYDLWLDPASEKERLLALLEPYPADLMQAEPVSMRVN
                     NVRNDDSECIAAEKELF"
     gene            12111..12518
                     /locus_tag="VFB80_09335"
     CDS             12111..12518
                     /locus_tag="VFB80_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN34010.1"
                     /translation="MTEEEPAANGRSPLPSLSTLGEDTARDLVQLFKLLADETRLRIM
                     YYLMQKDELNVRTLCRMLRQSQPAVSHHLALLRVAGIIECRRDGKHNFYHLVPKRCQA
                     YLDVVFGVRGDQQRKVRVEDALLTYGRDAQLAS"
BASE COUNT         2157 a         4137 c         4096 g         2216 t
ORIGIN      
        1 cggcgaaaaa gtacccggtc atcgaaaaga tttatgctgg cccgcagggc tcgttcactc
       61 cgaagagctt ttccacgcag ggcttgaact ccaccctgac cgacctgggc tttgtcgtcg
      121 tgcagatgga cggcatgggc accgccaacc gcagcaaggc gtttcacgac gtctgctggc
      181 acaatctggg ggacgccggg ctgcccgacc acatcctctg gcacaaggca gtcgccgcca
      241 aatatccgtg gtacgacatc agccgcgtcg gcatctacgg cggctcggcg ggcgggcaga
      301 attcgaccgg ggcgatgctg tttcagcccg atttctataa ggcggcggtc agcggatgcg
      361 gctgccacga caaccggatg gacaaggcct cgtggaacga gcagtggatg ggctttccag
      421 tcgggccgca gtacggcaag agctcgaaca tcgacaacgc cggcaagctg cgagggaaac
      481 tgatgctgat cgtcggcgag atggacacca acgtgccgac ggaatcgacc ttccggctgg
      541 ccgacgccct gatcaagaag ggcaaggatt tcgatttggt cgtcgtcccc ggcgcggggc
      601 acggcatggg cggatcgtac ggcacgcggc ggatgaacga ctttttcgtc cgccacctgc
      661 tgggcatcga gcctcccgac cgcaacgccg cgcccagcgg gcctagcggg gagtaaccgc
      721 cgacgatgga ctggcggaag tcaccgctgc ggtggatcgc ggctctggcc gcagctctga
      781 cggtgattca gccggcctgg ctgctgatcg agttcgcccg cggccgcggc ccgctgccgc
      841 tggtgctgct ggtgtttaat tacgcgttgg tagtcgtcat tacgctgtgg atcacgctcg
      901 acgcccgcga gcgccgccgc accccgtgct tcgattacgg gtttttcctg ctcattcttt
      961 ggccgttttc gctattctgg tattgcggct gcacgcgggg ctggcgcggg atcgggctgg
     1021 cctgcgtttt gtacgtgctg gcgtggctgc cggcgtttac cacggcggtc ataataatga
     1081 ttgcggcgat tgcagccgcc gttttgaatc cttgacgggc aaagcacgat gagtgggcgt
     1141 cgattcacac tgatcctctt cgcggcggcg gcactcgcca gtcaaacatc cgcccgcggg
     1201 cagcaggaaa tcgtcgccga gacgccgaag aacctgacgc cgatcgaccg gacgccgatc
     1261 gtcgaagcgc cgatcaccga cggtgatcgg gcgcattggg cgttcgcgcc ggtcgcgcgg
     1321 cagtccttgc cgcaggcgaa gattcctgcg tggccgcgga ccgccgtcga tgcgttcatc
     1381 ctggcgaagc tggaagccga aggtattgcg ccctcggcgg aggcgagccg agctacgctg
     1441 ctgcggcgat tgtcgttcga cctcaccggc ctgccgccga cgccgcagga gctggcggcg
     1501 tttcaagccg acgcgtctcc ggatgcctac gagcggcagg tcgatcgctt gctggcgtcg
     1561 ccggcgtttg gcgagcggta tggccagtac tggctcgacc tggcccgctt tgccgagacc
     1621 gacggctacg agcacgacaa ggtccgcgcc caagcctgga agtatcgcga ctgggtcatc
     1681 gccgcgatca atgccgatct gccgtacgac gaattcatcc ggcagcagat cgccggcgac
     1741 gaagtcgccg gttcaaagtt caaagttcaa ggttcaaagt cggaaccaac aaccttgaac
     1801 tttgaacctg gaaccttgaa ctcgagcgca gcgagcatgt tctgtctgtc cggccccgac
     1861 atgccggaca tcaacgacca gctggagcgg cggcatagct tgatgaacga gctgactgcg
     1921 accgtggggt cggcgttcct cgggctgcag ctgggatgtg ctcagtgcca cgaccacaag
     1981 tacgatccgc tcagccaggc cgatttttac cggctgcgag cggtgttcga gccggccgtg
     2041 ccggcgctca agcgcgacgt gccgattagc gctctggcgt cgcaaaagga gaccgagtcg
     2101 gcgcggttgt gggtccgcgg cgatcaccgg cggccagggc tggaggtgca gccctcgttt
     2161 ccacggatcg cctcggcgcc aacggggcgc gccggcgagg gagtgcatga ggagtccgat
     2221 acaccctcgc tcgcgcgtcg ggctagcgtc cgcagcgagc tcgccgactg gctcgtgcgg
     2281 gccgacaacc cgcttactag ccgggtgatg gccaaccgcg tctggcaagg gcactttggc
     2341 cgcggcattt gccagacgcc gagcgatttc ggcgtgatgg gcggcccggt gacgcatcgg
     2401 gagctactcg actggctggc tgccgagctg cgcgaaagcg gctgggggct gaaggggctg
     2461 caccggaaga tcgtcacggc ggcaacctac agagagattt ccgatttccg atttccgatt
     2521 tcagatttgg aggcaaaggg ctccgcaaat cttagatctg aaatcctaaa tctgaaatcc
     2581 gagctctacg gccgctatcc ccgccggcgc ctcgagggcg aggcgattcg cgatgcgatg
     2641 ctggcggcgg cgggcttgct cacgagcgag cgcggcgggc cgggcgtgct gccgccgctg
     2701 ccggaggaac tggtcgggac gctgctcaag ggccaatgga ccgcgagcca gcgcgaggcg
     2761 gaccactaca agcggagcgt ctacgtcttt gcccgccgca acctgcggta tccgatcttc
     2821 gaggcgttcg accgccccga cggcaatgcc agctgcccga ttcgcaacaa gtccacgacc
     2881 gcgccgcagt cgctgctgct cttcaatagc gaattctcgc tgctagccgc caggagactc
     2941 gccgggcgag tcatgaatat cgaagaaccg aagaaccaaa gaaccgaaga gcagatcgag
     3001 cggctgtacg agattgccct ctcgcgccag cccaccgcgg cggaaatcac cacgttcagc
     3061 aaattcctcg ccgaccagca gcagcggcta aaggcggaag ctcgccccgt caaggaactg
     3121 gccttgcccg ttccttgccc cgacacggcc gacgccgatg ccgccgcggc actcgtcgat
     3181 gcctgcctgg ccgtgctcaa tgcgaacgag ttcatttacg tcgattgacc ggcgagcggg
     3241 aggcgtaagc ctcccgatca tcttcacaac cgcagccggg agaatttggc gggcagcgat
     3301 atcgggaggc ttacgcctcc cgctcgccgg ggaatcttcc tattgctttc caggtgcaat
     3361 cccgtcgcct aattcggcga gaaacccagg atggatccaa ttcgccaagg gatattcctg
     3421 atcccgaaca taggccacag ctccgagaac tgcagcttcg ttctttagct tgcgcttcga
     3481 gccagactcg gtccaccagg tcccgctggc tggccgctga aaccctcgat tcaacatgcg
     3541 gctggcatag ctcttcaagt cgcgaaggat cgcttccggc ggcggatcgc cgggcacgcc
     3601 cacgacgaca tgaacgtggt tcgccatggt cgccgcggcg atcagttgcc attggcggta
     3661 gtttgccgtc tcgtgcaatt gcgacaggac ggtctgtgcc tgctgggacg tcagcagaat
     3721 tggggcacac ttcaggcgat tccgagcgtc atgagccagg tgctcccaat ccgcgtcgta
     3781 aggagcttga ggaatgttgt gaatgacccg tccaccttgg ccgtcattaa cctcgctgac
     3841 aaacccacgc ttatcgcccg gcaaccaggt tccataggtg gtccaagtta gaagccagaa
     3901 gcggtccata ggagcatgat ggcgaatggg aggcttacga gtcccgctcc cgctttcgcc
     3961 gtgaacctgc tgggcggcgt acagccatcg ggaggcttac gcctcccgct cgccggccat
     4021 tttgctccgc cccgcgtact tcctggccag actcacactt gaacggcagc tcgcggtagt
     4081 cgtcgccaaa gcagacgccc agggcccggg cggcttgcac ggccgagcag ttggggcaga
     4141 cgctggagag cttgatcgcg tcggcaatcg ctacgtcgtt caggtccggc ggttggtacg
     4201 tgaccatgtg gccgaacttg cgctcgacga tcagccgcac ggcgtgagcg ccgaactgcg
     4261 tggccagcac gcggtcgaaa gtcgtcggcg ccccgccgcg ctgcaggtgg cccagcacga
     4321 ccgtgcgggt ctccttgccc agccgccgct cgatctcccg cccgacgaac gtgccgatgc
     4381 ctcccaggcg gacctgcttc tgacattcct gcttgtcctg cgtcaccagg ccgccgtcgg
     4441 gcagtttcgc gccctcggcg acgacgacca gcgtgaagcg cttgccgcgc tgttcgcggc
     4501 cgatgatcgc ctgacagacg ttctcgaacg tccagggaat ttccggaatc aggatcacat
     4561 cgccgccgcc ggcgatcccc gcgtagaggg cgatccagcc cgcgtgccgg cccatcactt
     4621 ccagcaccat cacccgctcg tggctggcgg ccgtcgtatg tagccggtcg agggcgtcgg
     4681 tgccgcaaaa gacggcgctg aagaagccga acgtgaaggc cgtgctcgac aggtcgttgt
     4741 cgatcgtctt ggggacgccg acgacgggaa tgccctgctc gtgaaactgc tgggcgaccg
     4801 cgagcgagcc gtcgccgccg acgcagatca gcccgtccag gctaagttgc tcgatggtcg
     4861 tcttcacgcc ggcgatcagc tccggatcga gcgtcaggcg gtcgttgacg ccgatcgttg
     4921 ccgcgaagcg ccccttgttg gtcgagccga ggatcgtgcc gccctggccc aggatgccgg
     4981 ccgtgttctt gtgcgtcagc gggatgaagc tgaccggatc gaccagccct tcgtagccct
     5041 tgaggaagcc gatgacgtcg tagccgtgtg tgttggcgct tttgacgact ccgcggatca
     5101 ccgcgttcag gcccgggcag tcgccgcccg cggtgaggat gccgatccgc cggccctgcg
     5161 aagtctggtc ggtcatggcg cgcctgctgc ccagggagaa gatcgagcaa ctctacgttg
     5221 gactccggcc gctggaggcc cgtttttggc ccttgcgtta caacccgcgc agctggaata
     5281 tggagaggca acctgagtct cgccccgcgc ctaacttgcc gcaagtgggg aatcgctggt
     5341 gcattagcgc cgttcatttg acgcaaaggc gcgaaggcgc agagcggcaa agaatgcgga
     5401 agctcggaga gaaaccgatg agtgccgacc cgctccgccg atctaattcc ggcccgcact
     5461 ctgaaccttc gcgcctccgc gactttgcgt taagggcctg gtctgtggcg ctcgccgcgt
     5521 tgtcgattct gcgtgtcgcc gcggcggatg aattgaaaga gacggccgtc ttcacggcgg
     5581 gcgcggaagg ctatcacacg tatcgcatcc cggcgctgct ggtgacaaaa gggggcacgg
     5641 tgctggcgat ttgcgaaggc cgcaagaccg gaacggggga ccatggcgac ctggatctcg
     5701 tgcagaagcg gagcaccgac ggcggcaaga cttggggacc gctggagctg atccacgaag
     5761 agggcggcac tgccaaggtc accatcggta atccctgccc ggtcgtcgat caatccaccg
     5821 gcgtgatctg gctgccgttc acgcgcgaca acgatggcgt gttcgtgatg tcgtccaagg
     5881 acgaaggaaa aacctggtcg cagccgcgcg agatcacggc cgatgtcaaa ccaaaggaat
     5941 ggaattggta cgcgaccggc cccggcaacg gcatccagct caagagcggc aaatacgccg
     6001 gtcgactggt gattccctgc gaccaccgcg tcggcaccgc gaccaaggac gacaaaaccg
     6061 gttgggacaa ggcgggccgc tcgcacgtca tttattccga cgaccacggc gcgacctgga
     6121 agctgggcgg ggccaccgat tactccatga acgagtgcgc cgtgatcgag cgggccgacg
     6181 gctcgttgct gctcaatagc cgcagctatc gcggcaaggc ctgccgcggc gtttcgatca
     6241 gcaaggacgg cggcgcgacg tgggagccga caaccgacga tccagccctg gtcgagtccg
     6301 tctgccaggc gagcatgatc cgcgccgagg gcgctgccgt gctgttcagc aaccccgcca
     6361 cgccccgcgg ccggcaccac ctcaccgtcc ggctgagcct ggacgacggc cagacctggc
     6421 cgcactcgcg gctgatctgc gaaggccctt ccgcttattc gtcgctggcg gtgctgcccg
     6481 gaggcgaaat cggcctgctc tacgagcagg gcccgtacaa gtcgctcacg ctcgcccgct
     6541 tcccgctcgc ctgggtcaag acggcccaga attgagcgcc tgctcgcaat cgacgttgcg
     6601 acatttgacg ccaaggtgca gagacgcaaa gacgacagga aacaggagag gatgttttag
     6661 gatgagagtc gattcccgcc acatgcctgc gctttgtttg ttccgcctct ttgcgcctct
     6721 gcgcctttgc gcccttgcat caaatcgggc ggcgtcagtg ttgacactcg cgctccttct
     6781 tggatcgatc gccgccgccc agaccgaccg gtctcccgtc gatctggtgc tcggtccgga
     6841 cgagacgtgg ctggcgacgg tgaatcaaac ggccgattcg gtttcactgg tgcgagtcag
     6901 cgacggcaag gtgctcgatg aagccgcggt cggcgatcat cccatcggga tcgcgctttc
     6961 cgccaacggc aagacgctgc tggtcagttg tcatcattcg aatgaggtcg cgctgctgga
     7021 agtccgcggc gagaaactcg ccaaggccgg cgcgatcgac gtgggatatc agccgcatgg
     7081 gattgcggtc gctgagggtg gccgcacggc gtatgtcgcg tgcacggcca gcgcggaggt
     7141 ggcggtgctc gacttgcagc agaagcaggt cgcgagccgg attgcggtcg gccgctggcc
     7201 gcgcaacctg gccctctcgc gcgacggccg ccggctgatc gtcggcacca gcggcgaccg
     7261 cggcgtgacc ctcgtcgatt gcacggagcg aaaaatcgtg cagcaggaac agttcgtcgg
     7321 cctcaacatc ggccactttg ccgtgaccaa ggacggccgc cacgcgtact tcccgtggat
     7381 ggtttaccgc aacaacccga tcaccccggg caacatccgc ctgggctggg tgctggctag
     7441 ccggatcgcc cgcgtgcggc tggatgaggc ctcgcgccgc gaggcgatgt cactcgaccc
     7501 gcagggaaag gcgatcgccg acgtcgaagg catcgctctg accagcgacg acgccaggct
     7561 gctcgttacg gcctccggca cgcacgagct gctggtctac aagaccgagg gcctgccgct
     7621 gaaggactac ggcggctcgg accatatcga tcccgcgctg ctgaaggacg acgagcggtt
     7681 ccaccgcatc gagctgggcg gccggccgat gggcctggcc gtcgccaagg atgaccggac
     7741 cgtttacatc gccaactatc tggataactg cgtgcaggtg gtcgatctgg cggatcgcaa
     7801 ggtcattcgc acgctgccgc tgggcccagg ggccgaaccg tcgctggccc gccggggcga
     7861 ggcaatcttc tacgacgccc gccgcagtct cgaccagtgg tacagctgcc acacctgcca
     7921 ctacgaaggc ggcaccaaca gcattcccac cgacaccacc aacgacggca ccgcgttcac
     7981 gttcaagacc gtgctgccgc tgcacaacct gcgcgaaacc ggcccctgga cctggcacgg
     8041 ctggcaaacc gacctcgccg ccgccatgaa aaagtcgctc accgaaacaa tgctcgggcc
     8101 tccgccggct gaaggggacg ccgacgccct cttggcctat ctcgattcgc tcaagctgcc
     8161 gccgaatccg ttcgtcggca aggacggcgc gctctccgcg tcggcggagc gcggcaagct
     8221 gctcttcgcg ggcgacaagg ccgcctgcgc gtcttgccac agcggaagct atttcaccga
     8281 cggccaggtc cacgacgtcg gcctcggctc ggcccgcgac cgctacaagg gctacaacac
     8341 gccgtcgctg ctgggcctct atcgccgcgt caagttgctg cacgacggcc gcgtcgattc
     8401 gctcgagcag ctcctcgccg gcccccacgc gcccgagaaa gtctccggca gcaagctgag
     8461 cgacgatgag ctgcgggatc taatcgaata tctgaagacg ctgtgaacgg cggcgcggat
     8521 ttggatttcg gtagcaatcg catggcaggc ggcgggagac ctcgctcgtg catgacattc
     8581 cgttgatctt gacggtgacc tacggcctgg cctttgcgct gctctttgga accatcacga
     8641 atcgcttcgg gctctcgccg atcgtgggct acctcttggc gggcttcatg gtcgggccga
     8701 agacgccggg ctttgtcgcc gacccgaaaa tcgccgcgca gctggccgag ttcggcgtca
     8761 tcctgctgat gttcggcgtc ggcctggaat tccactggcg ggacttgctg caggtgaagt
     8821 cgctggccat tcccggggcg ctcttccaga gtgcggtcgc gacggccctc tgcacggcga
     8881 tggctccgct gtttggcatg tcgttggcct cgggcgtggt gctgggcatc gccgtctccg
     8941 tcgcgagtac cgtcgtgctg gtccgtctgc tgatggacaa cggcgtgctg cacacgccag
     9001 aggggcacgt cgccgtcggc tggctggtcg tggaggacct gttcaccgtg gtggtgctgg
     9061 tgctactgcc ggcgctgaac gcagccctgg cggagggagg ggcggcggcc atcgccggct
     9121 cgatcgcctg gaccgccgcg aaaatcgtcc tgctggtcct gctggtgctg gtgatcggcg
     9181 cgcgggccgt gccgctgctg ctgaactaca tggcccgcac gcggtcgcgg gaattgttca
     9241 cgttgtcggt gctggccgtc gccctgggaa tcgcgaccgg ctcggcgctg gtgttcggcg
     9301 tgtcgatggc cctcggagcg tttctggccg gcatggccgt cggccagtcc gacgtcagcc
     9361 agcaagcagc ctcggacgcc ctgccgatgc gggacgcctt cgcggtgctg ttcttcgtct
     9421 cggtcggcat gctcttcgac ccggcctcgc tcgccgagca ctggccgctg gccctggccg
     9481 tgacggcggt catcctcatc ggcaagccgc tggcggcgct ggccatcgtg ctggtcctgg
     9541 gctatccgat caggactggc ctgaccgtgg ccgtggccct ggcccagatc ggcgaattct
     9601 cgttcatcct cagcgaacag gctcgctcgc tgggtttcct gtcgcaggcg gcgtcgagcg
     9661 tgctcgtgac ctgctcgatc atctccatca cgctcaatcc gctactcttc aagctggtcg
     9721 agcccgccga gcgctggctg aagggccggc ccggcctgct caggcggctg gcccggcggg
     9781 ccaagtcgaa tcccctggcc gaaatcgccg cgctcgaaac ccacggaaaa gtggctcagg
     9841 cgatcgtcgt cggctacggg cccgtcggcc aaacgctgac tgccatcctc cgcgactttc
     9901 acgtcagccc ggtcatcatc gacttgaatg tagacacggt gcggcggctg cgccacgagg
     9961 gctggcaggc gatctatggc gacgcccgca gcaccgagat tctccgcaac gcccagatcg
    10021 aaaaggtgcg gtacctgctg ctcacgctgc ccgacgaggc ggcccgcctg gcgatcatcg
    10081 ccaccgccct gtcgctgaat ccggagctca agatcatcgt ccgcgcccgc tacctggccg
    10141 agcgcgacgt cctgcgcgag gcggacgtcg atgccgtagc cttcgaggag gcggaagtcg
    10201 ccgctgccct cggcgaaatg ctgctcaagg aaatgggggc tgacgaggac gaagtgctcg
    10261 ctcgctcggc gaagattcgc gccgacctgt ccgtgggcgg caaaccctag cggcggacaa
    10321 ttagtgctgc ccgccgcccg ccggtttgct ctcgcgctcc ggagcgggaa cgctccaggg
    10381 catgccgtcg tcgcggtcca tcgaaaaatc gctgaacgcg acgtccacca ccagcggctc
    10441 ttgcagcacg cggttgatcg cccgcacgac ctcggccttg aaatactcca gccccggatc
    10501 ggtcagatac tccgtctcgc tccgctgcac gagcgaaatc actgcgtcgc ggacccgctt
    10561 ttcgaccggc ggcagcagct cggccaggcg ctcctgccgc gcctgcggga ggatcgcgac
    10621 gagctgaaac tgcacgtgca tctggccttc gccagctgcc agcggatgcg ttatgcggaa
    10681 ccggcccaag tcggtctcga cgtggttccg gggatccagg tgctgctggt cctgaatcag
    10741 cgcgagcaat tcgctggcgt ccagctcggc caccgaccgc ggcttctcgc cgcagcctgc
    10801 gatcaagaag cccaggagca ggacgagaat caggatggtt gcaatgctgc gcacggattc
    10861 cacttcctct ttgccgcttg gcgagacctg ccacgctcgg aaaccctagc atgcgcgtcg
    10921 ggccgcttat gccggtcgtg cggctcagaa cagctctttc tcggcggcga tgcactcgga
    10981 gtcgtcattc cgcacgttgt tcacccgcat gctcaccggc tcggcctgca tcagatcagc
    11041 aggatacggc tccagcagcg ccagcagtcg ctccttttcg ctggccgggt cgagccacag
    11101 gtcgtagtcg gccggatcca agatcacggg catccggtcg tggacattcg ccgaaagagt
    11161 gttggcggag gtcgtgacga tcgtgcagga ttccagcggc ggcgagtccg gcggcgttcc
    11221 ctccggcccg cgccaccgct cccacaggcc ggcaaaggcg aacggttgcc cgctcttcag
    11281 ctggaacaag agcggctgct tcttcttgcc gaccgctttc cattcgaagt agccgtcggc
    11341 cagcaccagg cagcggcggc gggaaaacgc ggtgcgaaac gccggcttct ccgccagggt
    11401 ctcgccgcgg gcgttgattg tgctggaggc cagcttggcg tccttggccc agcttgggat
    11461 cagcccccag tgaaacaggg ctagctgccg cgctccgccc gcttccagcc gccggattcc
    11521 cgcgaccgtt tgcgtgggag caatgttgaa ccgcggcttg agctcgagat cggccgggag
    11581 gtcgcccagc tcgaacagga actgctgggc cagtacgtgc agcggcgtcc gcagcgtata
    11641 gcgtccacac atggttggtt cgagaaatag ccggggccta tggcggcaaa agggatgccc
    11701 aagggctgct tctgaggctg ttttgccgac gaaaacgccg caaaatctgc cgccgggccc
    11761 cgtgggctag cacgaaacgt ttgaaagttg ttgaaaaaac tgtctgaaat ctgcgccgcg
    11821 actattctca atcgcggggg cggttgctta caataccggc gtacaagagg cgcttgccgg
    11881 actgagttcg aaacaggccg cgttctgcgg cccgctccgg caagcaagcg gatttggctc
    11941 atcacattgt attcccgacg gcccttcggg gttcctgttt tccttccccg cccgcgctgt
    12001 cgccacgcgc gccggctcgc gggcgtccgt tctcgtagag gagaggggaa tcgcggccca
    12061 tccgcagatt ccagcccgag gcattcatgg taacgcttac ttcccggcct atgactgaag
    12121 aagaacccgc cgccaacggg cgcagcccgc tgccgtcgct gagcacgctc ggcgaagaca
    12181 ccgcccgcga cctggtccag ctcttcaagc tgctggccga cgaaacgcgg ctgcggatca
    12241 tgtattacct gatgcaaaag gacgagctga acgtgcggac gctctgccgg atgctgcggc
    12301 aaagccagcc ggcggtgagc caccacctgg ccctgctgcg ggtggcgggc atcatcgaat
    12361 gccgccgcga cggcaagcac aacttctatc acctcgtgcc caagcggtgc caggcatacc
    12421 tcgacgtcgt cttcggcgtt cgcggcgacc agcagcgcaa agttcgcgtc gaggacgccc
    12481 tgctcaccta cggccgcgac gcccagctcg cgtcgtaagt ggggcaggct cacccagcct
    12541 gccgcctgag tgacatggcc gcttcgctgg cggctaaact ggcggacggt tctccacatt
    12601 ctcgga
//
LOCUS       DASRJB010000356         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2067237, whole genome shotgun sequence.
ACCESSION   DASRJB010000356 DASRJB010000000
VERSION     DASRJB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067237"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFB80_09340"
     CDS             complement(<1..412)
                     /locus_tag="VFB80_09340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN34011.1"
                     /translation="MSRRVVLTGAGRGLGLAMTEKLAELGHTVIGCSRQPAHALSLKK
                     RFGGPHHFDAVDVADDAAVGKWAKGVLADGGPPDLLLNNAAVVNANAVLRDVPQDEFS
                     RVIDVNVKGVYHVLRHFLPAMIKRKAGVIVNFSSG"
     gene            546..1076
                     /locus_tag="VFB80_09345"
     CDS             546..1076
                     /locus_tag="VFB80_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZN34012.1"
                     /translation="MSQAPRYCPQQPLPPYSYVTGLAPHPTRDPAGHSFGRHEARPPP
                     LDEANFRDNPAYLFGLDLFNHGFYWEAHEEWESLWHAAGRKGAAADVLKGLIKLAAAG
                     VKAREGRAEGVRRHARRAEELLRPAHQGGRQHCFGLFLEDLIGAAQSIAASADSLAGD
                     GRADATRRLPLTLRLE"
     gene            complement(1085..1573)
                     /locus_tag="VFB80_09350"
     CDS             complement(1085..1573)
                     /locus_tag="VFB80_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN34013.1"
                     /translation="MPILLADLSNPAHQAAIVELLDMYCRDEFGDGRPLSDYARENLI
                     AGLARHGGARVFLAYDDQRSPPTETQRPVGLALCFVGFSSFKARPLINIHDIAVRPEV
                     RGRGIGRQLLDAVRDHARASGCCKVTLEVRADNHRAQQAYRRAGFQSTQPETWFWSLP
                     IS"
     gene            complement(1646..2578)
                     /locus_tag="VFB80_09355"
     CDS             complement(1646..2578)
                     /locus_tag="VFB80_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HZN34014.1"
                     /translation="MQRFDSQLASLINLAARLCQATEAGSVLVMLEGPTEWPLLKQAV
                     GELQVVVAADTAEDLAGADEAGLATIVLNMADSPVIEKLTQALLTGVAREVLSPGARV
                     VVVYSGFEVDTIDSISFIELDEHLGRLTARDLRQLETSVPLETLKTVIDLAVEIGREG
                     REGKAIGTMFVVGDTRKVLAHCQPAGFDPVKGYSRTERDLHDARVREAVKEVAVLDGA
                     FIINADGTVEKAAQLVDAPYANLTLSKGLGARHWAGAAISKATGSLAIVVSQSSGTVR
                     LFQSGEVMLRIEPLRQAMKWKDFDYEPPPAGEAE"
     gene            2762..>3606
                     /locus_tag="VFB80_09360"
     CDS             2762..>3606
                     /locus_tag="VFB80_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34015.1"
                     /translation="MKRYYTPPVSSLSRREFALAGLAGLSALMVNPARGQLSARKMRV
                     AAINSIFRLRSHAYHIVGRMVYGFQMDGLHHQPDMQVVRMYNDQVPPADLSQSFCQRH
                     GIELVKTPAAALGGAELDVDAVALICEHGDYPVNEFGQILYPRYELFQQIVEVFEKSG
                     RSVPVFVDKHLSYDHQKAAKMVATAKRMGFPLMAGSSLPVTWRVPQVELPRASPLVEG
                     VCTFGYDRSTAEIYLFHGLEVLQCMLERRRGGETGVKTVTCLQGDAVWKACDDGRITW
                     SLVEES"
BASE COUNT          591 a         1151 c         1240 g          624 t
ORIGIN      
        1 agccggagct gaagttgacg atcacgcccg ccttgcgctt gatcatcgcc ggcagaaagt
       61 gccgcaggac gtgatagact cccttgacgt tcacgtcgat gacgcggctg aactcgtcct
      121 gcggcacgtc ccgcaggacc gcgttggcgt tgacgacggc cgcattgttc agcagcagat
      181 cgggcggccc gccatcagcc agcaccccct tggcccattt accgactgct gcgtcgtcgg
      241 cgacgtcgac cgcgtcaaag tggtgcgggc cgccgaagcg ctttttcagg ctcagggcgt
      301 gggccggctg ccgactgcag ccgatgacgg tgtggccgag ttcggccagc ttctcggtca
      361 tcgccaggcc cagcccgcgg ccagcgccgg tcaagacaac tcgacgactc atgtctttag
      421 ccgggaccaa acctggtccc ggagcggagg gaaaacgtta tcagatggaa tgtgcacgct
      481 cgcttcctgc gcgaccactg gtcgcggctt tgaacgcgat gggattttac ccgacaatgc
      541 cagtcatgtc tcaggcgccg cgctattgcc cgcagcagcc actgccgccg tacagctacg
      601 tcacgggcct tgcgccgcac ccgacgcgcg atccggcggg gcacagtttc ggccggcatg
      661 aggcgcgacc tccgccgctc gatgaggcaa acttccgaga caaccctgcc tacctgttcg
      721 gcctcgacct gttcaaccac ggcttctatt gggaggccca cgaggagtgg gagtcgctgt
      781 ggcacgccgc ggggcgcaaa ggcgcggcgg ccgacgtcct gaagggcctg atcaagctgg
      841 cggcggccgg cgtgaaggcc cgcgagggtc gggcggaggg agtgcggcgg catgctcgcc
      901 gggcggagga gttgttgcga cctgcacacc agggcggcag gcaacactgc ttcggcctgt
      961 ttctggaaga tctgatcggt gcagcccagt cgatcgccgc gagcgcggac tcgctggcgg
     1021 gcgacggccg ggccgacgct acccgccggc tgccgctgac gctgcggctc gagtgaacgc
     1081 gacgctagga gatcggcagc gaccagaacc aggtttcggg ctgcgtcgat tgaaagccgg
     1141 cgcggcggta cgcctgctgg gcgcggtggt tgtcggcgcg gacttcgagc gtgaccttgc
     1201 agcagccgct ggcgcgggcg tgatcgcgga cggcatcgag cagctgccgg ccgatgcccc
     1261 ggccgcggac ctcgggccgc acggcgatgt cgtggatgtt gatcagcgga cgggccttga
     1321 acgacgagaa gccgacgaag cagagggcca gtccgacggg gcgctgtgtt tctgtcggcg
     1381 ggctgcgctg gtcgtcatag gccaggaaga cccgcgcacc gccgtgccgc gccagacccg
     1441 cgatcaagtt ctcgcgggca tagtccgaca gcggccggcc gtcgccgaac tcgtcgcggc
     1501 aatacatgtc gagcaactcg acgatcgcgg cctgatgcgc gggattcgac aggtcggcga
     1561 gcagaatggg catagttccg agttccgagt tcaaggttca aagttggagc agattaactt
     1621 tgaactttga actcggaact cgcaactact ccgcctcgcc cgccggcggc ggctcgtaat
     1681 cgaagtcttt ccacttcatc gcctgccgca gcggttcgat gcggagcatg acttcgccgc
     1741 tttgaaagag gcggacggtg ccgctggact ggctgacgac gatcgccagc gagccggtgg
     1801 ccttgctgat ggccgcgccg gcccagtggc gtgcgccgag gcccttggag agcgtcaggt
     1861 tcgcgtaggg agcatcgacc agctgagcgg ctttctcaac cgtgccgtcg gcgttgatga
     1921 taaacgcccc gtccagcacg gcgacttcct tcaccgcctc gcggacgcgg gcgtcgtgca
     1981 ggtcgcgctc ggtccggctg tagcccttga ccgggtcgaa gccggcgggt tggcagtggg
     2041 ccagcacctt gcgggtgtcg ccgacgacga acatcgtgcc gatggccttg ccctcgcgcc
     2101 cttcgcggcc gatttccact gccaggtcga tcacggtctt cagcgtctcc agcgggacgc
     2161 tcgtctcgag ctgccgcagg tcgcgggcgg tcaagcggcc caggtgctcg tcgagctcga
     2221 tgaagctgat cgagtcgatc gtatcgacct cgaagccgct atagaccacg acgacgcggg
     2281 caccgggaga gagcacttcg cgggcgacgc cggtcagcag ggcctgcgtg agcttctcga
     2341 tgaccggcga gtcggccatg ttgaggacga tcgtggccag gccggcctcg tcggccccgg
     2401 ccaggtcctc ggccgtgtcg gcggccacga ccacctgtag ttcgccgacg gcctgcttga
     2461 gcagcggcca ctcggtcggg ccttccagca tgaccagcac cgagcccgcc tcggtcgcct
     2521 ggcacagccg ggcggccagg ttgatcagcg aggcaagttg cgagtcaaaa cgctgcatgc
     2581 cgaggggcgg ggccggagga gctgcggcgg gcgtggacgc gtcggcatgg tacgcagtcg
     2641 gctgcgattc agcaaccgct tccgcggctg aaaatccggc ctcgccgcca gattgcgtgg
     2701 gggagtctcc ggcgataggg ttggtcagca tttccacgtt ggggaactca aggacctatc
     2761 catgaaacgc tactacacgc cgccggtttc gagcctttcc cgccgggaat tcgccctcgc
     2821 gggcctggcc gggctgtcag ccctgatggt taatccggct cgggggcagc tgtcggccag
     2881 aaaaatgcga gtggcggcca tcaattcgat ctttcgcctc cgctcgcacg cctatcacat
     2941 cgtcggccgg atggtctacg gctttcaaat ggacgggctg catcaccagc cggacatgca
     3001 ggtggtgcgg atgtacaacg accaggtgcc gcccgccgat ctgagccagt cgttctgcca
     3061 acggcacggg atcgagctgg tcaagacgcc ggctgcggcc ctgggtgggg cggagcttga
     3121 cgtcgatgcg gtggctctga tctgcgagca tggcgactat ccggtaaatg aatttggcca
     3181 gattttgtat ccgcgttacg agctgtttca gcagattgtg gaggtttttg agaagagtgg
     3241 ccggagcgtg ccggtctttg tcgataagca cctctcgtac gaccaccaga aggccgccaa
     3301 gatggtcgcc acggccaagc ggatgggttt tccgctgatg gccggctcgt cgctgccggt
     3361 cacctggcgg gtgccgcagg tcgaattgcc ccgcgcatcg ccgctggtcg agggcgtctg
     3421 cacctttggc tacgaccgca gcacggccga aatctacctc tttcacggcc tggaggtgct
     3481 ccaatgcatg ctggagcgcc gccgcggcgg ggaaacgggc gtcaaaaccg tcacctgcct
     3541 tcaaggcgac gccgtctgga aggcctgcga cgacggacgg atcacctggt cgctcgtcga
     3601 ggaatc
//
LOCUS       DASRJB010000357        11850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1511572, whole genome shotgun sequence.
ACCESSION   DASRJB010000357 DASRJB010000000
VERSION     DASRJB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511572"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VFB80_09365"
     CDS             complement(<1..1399)
                     /locus_tag="VFB80_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN34016.1"
                     /translation="MKRETHALKSKRPAANSAPPSGDLARARRRLIIVALLFLAALAV
                     TAGVWADWYYGLPADAVATFVGGQTCAECHRAQHDEWRGSHHDLAMDVATDATVLGNF
                     NEATLTHHGVTSKMFRRDGKFFVNTEGPVGKLADFEVKYVFGVDPLQQYMVEFDRTPD
                     MPPGEIARLQVLRISWDTKAKKWFHLNPPDVKEKLDPTDELHWTGLAQRWNHMCADCH
                     STNLKKNYDVASGRYHTTFSEIDVSCEACHGPGSLHVQLAKAPSLFWDRQRGYALAKL
                     KALSNVPQVQACAPCHSRRRIVAGDYTAGCNYYDFYANEVLTADAYHADGQIMDEVYE
                     YSSFLQSKMYHKNIKCSDCHNPHSARLKHTGNQVCTSCHQHPAGKFDGEIHHRHPMTS
                     TGAACASCHMPQTTYMDVDPRRDHSFRVPRPDLSVTLGTPNACTGCHLRDEVLPGQTA
                     SASGGRQPPDAVPLKE"
     gene            complement(1475..3148)
                     /locus_tag="VFB80_09370"
     CDS             complement(1475..3148)
                     /locus_tag="VFB80_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34017.1"
                     /translation="MRPPRAGSTGETASLSAETPGHNVDTTVAQATPGPLAPLYQPGD
                     SIGRYQVRRLLGEGAFGRVYACWDDKLAREVAVKTPKRRLATDDQLAAFLREARAAGR
                     LRHPGIVQVFDVNQQESGEPYVVMELVTGESLAELARREQLSPKRIAELLANAASALA
                     HAHQNDIVHRDIKPSNIWIDQQGGVRLLDFGLALDDAQRWQHAGELAGTFYYMPPEQI
                     SRQAHRLDGRADIWSLGVILYELLTGRRPFSGSKLGELSDEIQNKDPRPPRQLNPAAD
                     PRLERICLKCLAKSPGERYATAADLAQDLRRAAAEPSQPVLLSPAFMIWVGVMILVGL
                     GAIFMATGGLNPPQPAGPDIVAGPTALAWGVDVFLAGAQEKAIHVNEQPVELVNGDAV
                     QIWAKLPEPRYLGVMWIGSDGQVGVLHPKTPADNRPLTEFRQPPERSKGFPVQGPGGV
                     ETAVLIVSPEPLPPTDKLAAALAPSAPFPSLGELVLLDGVDVQPKRLASDSEGQQLLA
                     RAGRRGIKTEAADLERNASVAQLEDWKAVAASELPAGAQLHYLAIPHAP"
     gene            3339..3950
                     /locus_tag="VFB80_09375"
     CDS             3339..3950
                     /locus_tag="VFB80_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZN34018.1"
                     /translation="MSAVATEHILVVPTEVFHRLGYFQGFSGDTERYLRGLMDPAHTS
                     YRPRAAMEQDPSFKQLIPYVIFRHRDAAGGVQLFQYTRGKGQGEARLHAKRSIGIGGH
                     ISADDSAAASAYEEGMRRELEEEVAINTSYSSRMVGLINDDQTEVGKVHLGVVHLVDV
                     ERPAVQPRESEIIDAGFRPVPELLADIARFETWSQICLKALFA"
     gene            3961..4875
                     /locus_tag="VFB80_09380"
     CDS             3961..4875
                     /locus_tag="VFB80_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN34019.1"
                     /translation="MSHASHIDRRSLLAGLAATGTAATLSNVGAAEPMADDPNWKVAK
                     GRIRQSVVAWCFKPMPVEDLARHAAAMGLQSVELAPPSDWPTLKKYGLTCAIAGSHGF
                     VKGWNDKANWDFCIEKITASINAAADFGCPSVITFSGMRGSLPAGSEGDEIGKKNFVE
                     GIKRVLPLAEQKKITLALEMLNSRVDFEMKGHPGYQCDSIEWAVDVCRRVGSPRLKIL
                     FDIYHVQIMQGDVITRLKQYKDDIVHYHTAGNPGRNELDDTQEIHYPGIMRAIAETGY
                     TGFVGQEFIPTWKDPVAALRHAARLCDV"
     gene            4878..5726
                     /gene="mutM"
                     /locus_tag="VFB80_09385"
     CDS             4878..5726
                     /gene="mutM"
                     /locus_tag="VFB80_09385"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZN34020.1"
                     /translation="MPELPEVETMRRGIAAIAGCRIAGAERLRCKRRPILMQPRPATL
                     VRRLAGQRIAEVGRLGKRVVLGLESGQRLIFEPRMTGLVLVADPPTQEHLRFRLDLAE
                     GPIPHVWYWDRRGLGSVRLLDAQQFARQFETGALGPDALALSSGELRARLAHSRRAIK
                     VALLDQRAIAGIGNLYASEILHLARVHPAKRCDLLHESQWEAIHTWMLEVLRLAIRHE
                     GSTLSDGTYRNALNQSGGYQNHHRVYDRAGQPCLRCGSTTIVRIVQAQRATFYCPYCQ
                     TKRQRS"
     gene            5824..6696
                     /locus_tag="VFB80_09390"
     CDS             5824..6696
                     /locus_tag="VFB80_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZN34021.1"
                     /translation="MRSRLLAICTLGMLLVGRSDAADHGDSPVLAMLGRSDAQITDSF
                     AFLRGENLVLALCTNPAIPTTVTSYRFAPDLTATFCIDNHSRVSRDDILDLVQFGGTI
                     ERPTAVAENVTLQVTFDDDGLPELHATGIAGKYRRDIKLFTGLRDDPFIRKPRAGRNV
                     AAIVIELPVEAVLGPRNDLLIWATTKVPDLEGPISEHGGRALRSMFNEPMNGLSPRDH
                     WRKLEAAPDVMVLDVLQASGFPNGREPTDDVIDLVIDIPGGTLPGEGPEFPTANDVPF
                     LAAFPYLALPHPAP"
     gene            6699..7751
                     /locus_tag="VFB80_09395"
     CDS             6699..7751
                     /locus_tag="VFB80_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34022.1"
                     /translation="MQPARSLLAALTLVQGLSLAALAADPPTALEKIAYYQRQIAEHP
                     KHYPAYARLGQAWLDRARQTYDPAALAEARKALRQSLAIQPTYEALHTLAATANYSHQ
                     FEESLRWCAQAAEAAPQDKSILAMRVEALLALGRTDEARAAIGTVGESAGDFYTAASL
                     AHWHASQGDRAAAAARFSEAAQLASAQQAKGLAVWATVSAAGIWLDGGDLPQAKPLLE
                     AAVKLDAEDSFLKIHLAELAAAESRPGEALKIYEDLLKRQSDPELHRRAFLLARQLND
                     KAAPDSQLAAAEKLCRRAIDAGEAFGLETLANLYCDAGRGDEALRLAEQNLKHKQDAA
                     AHETLARARRLVGGKP"
     gene            7830..8525
                     /locus_tag="VFB80_09400"
     CDS             7830..8525
                     /locus_tag="VFB80_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34023.1"
                     /translation="MSTPAWRVILIVVVCIVTPGASAADDLAAALGSTLAKNAGHARQ
                     WHAEKDFKSVAQTAGGLSLLAQLMQARSDDAAWQSATKNVAAAIGELQAAARGEDATK
                     CAAALDLLDKSIAAAQALSPAGQPQAPPRSPGVRPLMLAMDGIYADAKVALIAGNVAA
                     AKNQARVLAELGKLVSNSKMGEQWTTMAGGFSQAALTAANSSETDPQRFRSVLRGVSQ
                     RCDACHEVRDAGR"
     gene            8561..9607
                     /locus_tag="VFB80_09405"
     CDS             8561..9607
                     /locus_tag="VFB80_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HZN34024.1"
                     /translation="MAKRNETCPVCDGESLDFDRRAFLKVAGAAAGASLLPSALRAAD
                     SDKPTPETLVKQLYESLSAAQKKDVAFTWDHQHPQWGLLRTRISNNWHITQPQIRSDF
                     YTKDQQEMCRAIYEGLFQPDWIKRIDKQLQDDTNNKPWGSEQNIAFFGQPGEKFEMVM
                     TGRHITVRVDGNSIDGMAFGGPVFHGHQATRTTGLNEEIGHPGNIWWPQALEANKVFE
                     MLDGKQREKALVAQLPAEQSVGFRGKDGKFPGVTVADLSADQKAQLQKTIKLVFEPYR
                     NADQAEVLDCLKQQGGLDGCSLAFYAQGDIGDDGVWDNWRLEGPSFVFYFRGSPHVHM
                     WVNIGSDTSVKLNA"
     gene            9683..11356
                     /gene="ggt"
                     /locus_tag="VFB80_09410"
     CDS             9683..11356
                     /gene="ggt"
                     /locus_tag="VFB80_09410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN34025.1"
                     /translation="MLVLNLLFATLAAELPPNAAASTKGMVASVHPLATDAGVAALRR
                     GGNAIDAAIAAGLTLGVVDSHNSGLGGGCFILIRRADGKLFAIDGRETAPAKATRDMY
                     LRGGKPDPDLSQMGPLAVATPGALAAYEMALKAHGKLPLAELVLPAARIAEDGAPISR
                     PLASALAGNARVLRQYSGEGMPLLKADGKPYVQGEMLKQPDLARTYRAIAEQGTDWFY
                     RGPFAKAVGEWMTAHGGILAADDFAAYRPLEREPLVTTYRGRTIVGFPPPSSGGIHVA
                     QILNILEGFDLNAVYAKDAGEYHHLLAEAMKLAFADRAYWLGDADFARVPRGLIDKEY
                     SRSLAAKIDLAKATPVAGHGTPPAADERVFLKHTTHIATADAEGNWVAMTQTVNTGFG
                     SLVIVPGTGVVLNNEMDDFSIAPGQPNAFGLVGAEANAVAPLKRPLSSMSPTIVLVDG
                     QPVLSVGAAGGPTIITQVVQALVRSLDLGLPLDQALAQPRVHHQWSPGEVRHESGLPA
                     ELQNSLAGRGHQLKSVRNMGVTQAIVFDKASGQFIGVHDPRVPGKAAGP"
     gene            11455..>11850
                     /locus_tag="VFB80_09415"
     CDS             11455..>11850
                     /locus_tag="VFB80_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34026.1"
                     /translation="MSLIPAGTKAVAQTDDAPGRWKLETLTLAEGKQYRGVVLEESPE
                     SIEFAEIVQPAGKPMHAVIRGVPRPMASKLQRLEGPEHQELVARFARFRHRAVIEAGR
                     LEEVELEANDSGGAAVLLYDGPWFTLTSTA"
BASE COUNT         2033 a         3878 c         3953 g         1986 t
ORIGIN      
        1 gttccttgag cggcacggca tcggggggct gacgcccccc gctcgccgag gcggtctgtc
       61 ccggcagtac ctcgtcgcga aggtggcacc ccgtgcaggc gttgggcgtc cccagcgtca
      121 cgctcaaatc gggccgcggg acgcggaagc tgtggtcccg ccgcggatcg acgtccatgt
      181 acgtcgtctg cggcatgtgg caactggcgc aggccgcgcc ggtgctggtc atcggatggc
      241 ggtggtgaat ctcgccgtcg aatttgcccg cggggtgctg gtggcaggag gtgcagacct
      301 ggttgcccgt gtgcttcagg cgggccgagt gcggattgtg gcagtcgctg cacttgatgt
      361 tcttgtgata catcttgctc tgcaggaacg agctgtattc atagacctcg tccatgatct
      421 ggccgtccgc gtgataggcg tcggccgtga gcacctcatt ggcgtagaag tcgtagtagt
      481 tgcagcccgc ggtgtagtcg ccggcaacga tccgccgccg cgaatggcag ggggcgcagg
      541 cctgaacctg cggcacgttg ctaagggcct tcaatttggc gagggcatag ccccgctgac
      601 ggtcccagaa cagcgacggc gccttggcca gttgcacgtg caggctgccc ggcccgtgac
      661 aggcctcgca gctcacgtcg atctcggaaa acgtcgtgtg gtaccttccg ctggcgacgt
      721 catagttctt cttcaggttg gtcgagtggc agtcggcgca catgtggttc cagcgctgcg
      781 ccaggccggt ccagtgcaac tcgtcggtcg ggtcgagctt ctccttcacg tccggcgggt
      841 tgaggtgaaa ccactttttg gccttggtgt cccacgaaat ccgcaatacc tgcaggcggg
      901 cgatttcacc gggcggcatg tccggcgtgc ggtcgaattc gaccatgtac tgctgcagcg
      961 gatcgacgcc gaagacgtac ttgacctcga aatcggccag cttgcctacc gggccttcgg
     1021 tgttgacgaa gaacttgcca tctcggcgga acatcttcga cgtgaccccg tggtgcgtca
     1081 gcgtggcttc gttgaaattg cccagcactg tggcgtccgt ggccacgtcc atcgccaggt
     1141 cgtggtgcga gccgcgccac tcatcgtgct gagcccggtg gcactcggca caggtctggc
     1201 cgccgacgaa tgtcgccacg gcatccgccg gcaatccgta gtaccaatcg gcccagacgc
     1261 cagcggttac cgcgagagcc gccagaaaca gcaatgcgac aattatcagc cggcggcggg
     1321 cgcgagccag gtcgccgctg ggcggcgcgg aatttgcggc cggccgtttg gatttgaggg
     1381 cgtgcgtctc gcgtttcatg gaccggccgg gcgccagggg gtaatctgcc agcctattcc
     1441 atgctgcctg gcggcgatag gcgctattcc gccgctaagg cgcgtgggga atcgccaggt
     1501 agtgcaactg cgctccggcg ggcaactcgc tagccgctac ggccttccag tcctccaact
     1561 gcgccacgct cgcgttgcgt tccagatcgg cggcttcagt cttgatgccg cggcggccgg
     1621 cgcgggccag gagttgttgg ccctcgctat cgctggccag ccgcttgggc tggacatcca
     1681 cgccgtcgag cagcaccagc tcgccaagac ttggaaaggg cgccgacggg gccaaggcgg
     1741 cggccagctt gtcggtcggt ggcagcggct cgggactgac aatcaggacg gccgtctcga
     1801 ccccgcccgg accctggacc ggaaatcctt tgctccgctc gggcggctgg cggaactcag
     1861 tgagcggccg attgtccgct ggcgtcttgg ggtgcagcac gcccacctgg ccgtcgctgc
     1921 cgatccacat gacgcccaga taccgcggct ccggcaactt ggcccagatt tgcaccgcgt
     1981 cgccgttgac cagctcgacc ggctgctcgt tgacgtggat cgccttttcc tgcgccccgg
     2041 cgagaaacac atccacgccc caggccaacg cagtcggccc agcgacgatg tcaggaccgg
     2101 ccggctgtgg cggattcaat ccccccgtgg ccatgaaaat ggctccgagg ccgaccagga
     2161 tcatgacgcc gacccagatc ataaacgcgg gcgaaaggag cacaggctga ctgggctcgg
     2221 ccgcggcgcg gcgcaaatcc tgggccaggt ctgcggcggt ggcatatcgt tcgcccggcg
     2281 acttggcgag gcacttcagg cagattcgct ccagcctagg atcagcggcg ggattcaact
     2341 gccgcggcgg ccgcggatct ttgttttgga tctcgtcgct gagttccccc agcttcgacc
     2401 cggagaaggg ccgccggccg gtcagcaact cgtacaggat gacgcccagc gaccagatgt
     2461 cggcccggcc gtcgaggcgg tgggcctgcc gggaaatctg ctccggcggc atgtagtaaa
     2521 acgtgccggc aagttcgccc gcgtgctgcc agcgctgggc gtcgtcgaga gcgagcccaa
     2581 agtcgagcag ccgcacaccg ccctgctggt cgatccaaat gttcgagggc ttgatgtcgc
     2641 ggtggacgat gtcgttctgg tgggcgtgag ccagagccga cgcggcattg gcgagcagtt
     2701 cggcgatccg ctttggcgaa agctgctcgc gccgggcaag ctccgcgagg ctttcgcccg
     2761 tgacgagctc catcacgacg tatggttcgc cgctctcctg ctggttgaca tcgaaaactt
     2821 gcacgatgcc cgggtgccgc aagcggccgg ccgctcgagc ctcgcgcagg aaggctgcga
     2881 gctggtcgtc cgtggcaagt cgccgcttgg gtgttttcac cgcgacttcg cgagcgagct
     2941 tgtcgtccca gcaggcataa acgcggccaa acgctccctc gccgagcagg cggcggacct
     3001 ggtagcggcc gatcgagtcg cccggctgat agagcggcgc gagcggacct ggcgtcgcct
     3061 gggccaccgt cgtatcgacg ttatggcccg gcgtctcggc ggagagggaa gccgtttcgc
     3121 ccgtggaacc cgcgcgcggc ggtcgcactt cagtcggctc gagaggattc atagcggccg
     3181 tcaggcttcc gatgggcagc ctctacgtta ggtggccgcg gggaggaaag tcaaatccct
     3241 ttgcttgatc tcgcggctgg ttctcgatcc cggcaaagga gttatactgc ggcggcagga
     3301 cgggcttcgt ccgcagattc acgcgaaaag gagttcccat gtccgccgtg gccaccgagc
     3361 acatcctggt cgtaccgacc gaagtctttc accggctggg ctattttcag ggtttttcgg
     3421 gcgacaccga gcggtatctg cgtggactca tggaccccgc tcacacgagc taccggccgc
     3481 gggcggcgat ggagcaggat ccgagcttca agcagctcat tccctacgtg atctttcgcc
     3541 atcgcgacgc cgcggggggc gttcagctgt ttcagtacac ccgcggcaag ggacagggcg
     3601 aagctcgcct gcacgccaag cgaagcatcg gcatcggcgg gcacatttca gcggatgatt
     3661 cggccgcggc gagcgcctat gaagaaggca tgcgccgcga gctggaggaa gaggtcgcga
     3721 tcaatacgtc ctacagcagc cggatggtgg gcctgatcaa cgacgaccag accgaagtgg
     3781 gcaaggttca cttgggcgtc gtgcacctgg tcgatgtcga gcggccggcc gtgcagccac
     3841 gggagagcga gatcatcgac gccggctttc ggccggtgcc ggaattgctc gcggacatcg
     3901 cgcggtttga gacctggtcg cagatttgcc tgaaagcgtt gtttgcttga ggagagcagc
     3961 atgtcgcacg ccagccatat tgaccgccgg tcgctgctgg ccggtctagc cgcgacgggc
     4021 acggcggcga cgctgtctaa cgtgggagcc gccgaaccga tggccgacga cccgaactgg
     4081 aaggttgcca agggccgcat tcgccagtcg gtcgtagcct ggtgcttcaa gccgatgccg
     4141 gtcgaagacc tggcccgcca cgcggcggcg atgggtttgc aatcggtcga gctggcgccg
     4201 ccgtccgact ggccgacgct caagaaatat ggcctcacct gcgcgatcgc cggcagccac
     4261 ggttttgtca aaggctggaa cgacaaagcc aactgggact tctgcatcga gaagatcacc
     4321 gcctcgatca atgccgcggc cgacttcggc tgtccgagcg tcatcacgtt ctcgggcatg
     4381 cgcggcagcc tgcccgccgg ctccgaaggc gatgagatcg gcaagaagaa ctttgtggaa
     4441 ggcatcaagc gggttttgcc gctggccgag cagaagaaga tcacgctggc cttggagatg
     4501 ctcaattcgc gcgtcgattt cgagatgaag gggcatcctg gctaccagtg cgactcgatc
     4561 gagtgggccg tcgatgtctg ccgccgcgtg ggctcgccgc ggctgaagat cctgttcgac
     4621 atctaccacg tgcagatcat gcagggtgac gtgatcacgc ggctcaagca gtacaaggac
     4681 gatatcgtcc actatcacac cgcgggcaat cccggccgca acgagctcga cgacacgcag
     4741 gaaattcact accccgggat catgcgggcc atcgccgaga cgggctatac gggcttcgtc
     4801 gggcaggagt tcattcctac gtggaaggac ccggtggcgg ccctgcggca cgcggctcgg
     4861 ctgtgcgatg tctgagcatg ccggagctcc ctgaagtcga aaccatgcgc cgcggcatcg
     4921 cagcgatcgc cggctgccgg atcgcggggg ctgagcgttt gcgctgcaag cggcggccca
     4981 ttctcatgca gccgcggccg gccacgctcg tccgccggct ggccggccag cggatcgccg
     5041 aggtcggccg gctgggcaaa cgggttgtgc tcgggctgga aagcggacag cggctgatct
     5101 tcgagccgcg aatgacggga ctggtgctcg tggccgaccc gccgacgcag gagcacctgc
     5161 gattccgcct cgacctggcc gaggggccga ttccgcacgt ctggtactgg gaccgccgcg
     5221 gcctgggcag cgtgcggctg ctggacgccc agcagttcgc ccggcaattc gagaccggcg
     5281 cgctaggccc cgatgcgctg gcgctgtcgt ccggcgagct gcgcgctcga cttgctcaca
     5341 gccggcgggc gatcaaggtc gccctcctcg accagcgcgc gatcgccggc atcggcaacc
     5401 tctacgcctc cgaaatcctg cacctggccc gcgtccaccc ggccaagcga tgcgacctgc
     5461 tccatgaatc ccaatgggag gcgatccaca cctggatgct tgaagtgctg cggctggcga
     5521 ttcgccacga aggctcgacg ctctccgacg gtacgtatcg caacgccctg aaccagtccg
     5581 gcggctatca gaaccaccac cgtgtctacg accgcgccgg acagccgtgc ttgcggtgcg
     5641 gaagcaccac gattgtccgc atcgtgcaag cccagcgggc gaccttttat tgcccgtact
     5701 gccagacgaa aaggcaacgt tcgtagctgt cgcggatttt tcgaaaaaag ctggcggaaa
     5761 agccttattc aattagaatt ggcgaggttg actttcgcga atcttggttg aaggaaccta
     5821 ttgatgcgaa gccggctgct cgcgatttgc accctcggaa tgctgcttgt cggacgctcg
     5881 gacgctgccg atcacggcga ctctcccgtg ctggcgatgc tcggccgcag cgacgcccag
     5941 atcaccgatt cgttcgcgtt cctgcgcggc gagaatttgg tcctggccct gtgcaccaac
     6001 ccggcaattc ccactacggt gacgagctat cggttcgcgc ccgacctgac tgcgacgttt
     6061 tgcatcgaca atcacagccg tgtgagtcga gacgacattc tcgacttggt tcagttcggg
     6121 gggacgatcg agcggccgac tgccgtggcg gagaacgtga cgcttcaggt gacgttcgac
     6181 gacgacggcc tgcccgagtt gcatgccacg ggcatcgcag gcaaatatcg gcgcgatatc
     6241 aaactattta ccggcctccg cgacgatccc ttcattcgca agccgcgggc cggccgcaac
     6301 gtggcggcga tcgttatcga gttgccggtc gaggccgtcc tcggccctcg aaacgacctg
     6361 cttatctggg ccaccacgaa ggtgccggat ctggaggggc caatctccga gcacggcggc
     6421 cgcgcgctgc ggtcgatgtt caacgagccg atgaacggcc tcagcccgcg cgaccattgg
     6481 cggaagctgg aggccgcgcc ggatgtgatg gtcctcgacg tgctgcaagc gtcgggcttt
     6541 cccaacggcc gcgagccgac cgatgatgtg atcgatctcg tcatcgacat tcccggcggc
     6601 acgttgcccg gcgaggggcc ggagtttccc accgccaacg acgttccctt cctggccgcg
     6661 ttcccctacc ttgcgctgcc gcatccggcc ccgtgatcat gcagccggcc cgttccttgc
     6721 tcgcggccct gacgcttgtc cagggcctgt cactcgccgc gctggccgcc gatccgccga
     6781 cagcgctgga gaagatcgcc tattaccagc ggcagatcgc cgagcatccc aagcattacc
     6841 cggcctatgc ccgcttgggt caggcctggc tcgatcgcgc ccggcagacg tacgatccgg
     6901 cggcactggc cgaggcgcgg aaggctctgc ggcagtcgct ggcgattcag ccgacctacg
     6961 aggcgctcca caccctggcc gcgacggcca actattccca tcagttcgaa gagtcgctgc
     7021 ggtggtgtgc tcaggcggct gaggcagcgc cgcaggacaa gagcattctg gcgatgcgag
     7081 tcgaggccct cctggcgctg ggccgcaccg acgaggcccg ggccgcgatc ggcacagtcg
     7141 gcgagtcggc cggagacttc tacacggcgg cctcgctagc gcattggcat gcgtcccagg
     7201 gtgaccgggc ggccgctgcg gcccggtttt cggaggcagc ccagttggcc agcgcgcagc
     7261 aggccaaggg cctggccgtc tgggcgacgg tctcggccgc cggaatctgg ctcgatggcg
     7321 gagacctccc gcaagcgaag ccgctgctgg aggcggcggt gaaactcgac gcggaagatt
     7381 cgttcctgaa aatccacctg gcggagctcg ccgcggccga gagccggcct ggtgaggcgc
     7441 tcaagatcta cgaggacctg ctgaagcgtc aaagcgaccc cgagctgcat cgccgagcgt
     7501 tcctcctcgc ccggcaactg aacgacaagg ctgcccccga ctcccagctg gccgcggccg
     7561 agaagttgtg ccggcgagcc atcgacgccg gtgaagcgtt cggcctggag accctggcca
     7621 atctctattg cgacgcgggc cgcggcgacg aggctctccg cctggccgag cagaatctga
     7681 agcacaagca ggatgccgcg gcccacgaaa cgctcgcccg agctcggcgc ctggtcggcg
     7741 gcaagcccta gcggaaccgt cgctcgctcc tggggtttat tgtcgcaggc cgttgtaacg
     7801 ggcagtgggg attggtatta catcgtggca tgagcactcc agcctggcgt gtcatcctga
     7861 tcgtcgtcgt ttgcattgtc acgccaggag cgagcgcggc cgacgacctg gcagccgccc
     7921 ttggcagcac gctggcgaag aacgcgggtc acgcgcgcca gtggcacgcg gagaaggact
     7981 ttaagtcggt cgcccagacc gctggcggac tgagcctgct cgcacaacta atgcaagctc
     8041 gcagcgacga tgccgcctgg cagtcggcga cgaagaacgt cgcggcggcg attggcgaat
     8101 tgcaagcggc ggcccgcgga gaggacgcta cgaaatgtgc ggctgcgctc gacttgctcg
     8161 ataagtccat tgccgcggcg caggcgctgt cgccggccgg ccagccacag gcgccacccc
     8221 gcagtccggg cgtgcggccg ctgatgctgg ccatggatgg catttacgcc gatgccaagg
     8281 tcgcccttat cgcgggcaac gtcgcggcgg ccaaaaacca ggcccgcgtg ttggcggagc
     8341 ttggcaagct ggtgtctaat tccaaaatgg gcgagcagtg gacgaccatg gccggcggct
     8401 tctcgcaggc cgccttgacc gccgccaatt cgtccgaaac tgatccgcag agattccggt
     8461 ccgtgctgcg cggcgtctcg cagcgctgcg acgcctgcca cgaagtacgc gacgcgggcc
     8521 gctaacctca tagaccactc actattcagg agcatttcca atggccaagc gcaacgaaac
     8581 ctgcccggtc tgtgacggcg agtccttgga tttcgatcgc cgggcgtttt tgaaggtcgc
     8641 cggggccgcg gccggcgcga gcttgctgcc ctcggcgctg cgggccgccg actccgacaa
     8701 gccgacgccg gaaacgctcg tgaaacaact gtacgagtcg ctctcggcgg ctcagaaaaa
     8761 agacgtcgcc ttcacctggg accaccagca tcctcagtgg ggcctcttgc ggacccgcat
     8821 ttcgaacaac tggcacatca cgcagccgca gatccgcagc gacttctaca ccaaagatca
     8881 gcaggagatg tgccgcgcga tctacgaggg cctgttccag cccgactgga tcaagcggat
     8941 cgacaagcaa ctccaggacg acaccaacaa caagccctgg ggctcggaac agaacatcgc
     9001 cttcttcggc cagccgggcg agaagttcga aatggtgatg accggccggc acattaccgt
     9061 tcgcgtcgat ggcaacagca tcgacggcat ggccttcggc ggcccggtct ttcacgggca
     9121 ccaggcgacg cggacgaccg gcctcaacga ggagatcggc catccgggca acatctggtg
     9181 gccgcaggct ctggaggcga acaaggtctt cgagatgctt gacggcaagc agcgcgaaaa
     9241 ggccctcgtt gcccagttgc cggccgagca atcggtcggc ttccgcggca aggacggcaa
     9301 attcccgggc gtcaccgtcg ccgacctttc ggccgaccag aaagcccagc tccagaagac
     9361 catcaagctg gtcttcgagc cctaccgcaa cgccgaccag gccgaagtgc tcgattgcct
     9421 gaagcagcag gggggcctcg acggctgctc gctggcgttc tacgcccagg gcgacatcgg
     9481 cgacgacggc gtctgggaca actggcggct ggaaggcccc tcgttcgtgt tctacttccg
     9541 cggctcgccc cacgtgcaca tgtgggtcaa tatcggcagc gatacgtccg tgaagttgaa
     9601 tgcgtgacgc agggccgcga ccagtggtcg cggcttcggc aattcgcaag accgcgacca
     9661 ttgctcgcgg ctttgaggct ctatgctcgt cttgaacctg ctcttcgcca cgctcgccgc
     9721 cgagttgccc cccaacgccg ccgcttcgac gaagggcatg gtcgcgtcgg tgcatccgct
     9781 ggcgacggac gccggcgtgg cagctctccg ccgcggcggc aacgcgatcg atgcggcgat
     9841 cgccgccggc ctgactttgg gggtcgtcga tagccacaac agcggcttgg gcggcggctg
     9901 cttcattctg attcgccggg ccgacggcaa gctatttgcc atcgacggcc gcgaaaccgc
     9961 gccggccaag gccacgcgcg atatgtacct gcgcggcggc aagccagatc ccgatctcag
    10021 ccagatgggg ccgctagccg tcgcgacgcc gggcgctctg gctgcgtacg agatggccct
    10081 caaggcccac ggcaagctcc cgctggccga gttggtgctg cccgccgcaa gaatcgcaga
    10141 ggatggtgcg ccaatcagcc ggccgctggc ctcggcgctg gccggcaacg cccgcgtgct
    10201 gcggcagtac agcggcgagg gtatgccgct gctcaaagcc gacggcaagc catacgttca
    10261 aggcgaaatg ctcaagcagc cggacctggc ccgcacctat cgcgcgatcg ccgagcaagg
    10321 gacggactgg ttctaccgcg ggccgttcgc caaggccgtg ggcgagtgga tgaccgcgca
    10381 cggcggcatc cttgcggcgg acgacttcgc cgcatatcgg ccgctcgagc gcgagccgct
    10441 ggtcacgacc tatcgcggga ggaccatcgt cggctttcca ccgccctcca gcggcggcat
    10501 tcatgtcgcc cagattctga acatcctcga aggcttcgac ctgaatgcgg tttacgccaa
    10561 ggacgccggc gagtatcacc acctgctcgc cgaagcgatg aagctggcgt ttgccgaccg
    10621 ggcctactgg ctgggcgacg ccgattttgc ccgcgtcccc cgcggcctga tcgacaagga
    10681 atattcccgg tccctcgccg cgaagatcga tctggcgaag gcgacgccgg tcgccgggca
    10741 tggcacgccg cccgcagccg acgagcgggt cttcctcaag cacaccacgc acatcgccac
    10801 ggccgacgcc gagggcaact gggtcgcgat gacccagacg gtgaacacgg gcttcggctc
    10861 gctggtcatc gtgcccggca cgggcgtggt gctgaacaac gagatggacg atttttcgat
    10921 cgcccccggt cagccgaacg ccttcgggct ggtcggggcc gaggccaacg cggtcgcgcc
    10981 gctcaagcgg ccgctgtcga gcatgagtcc gaccatcgtg ctggtggacg gccagccggt
    11041 gctgagcgtc ggtgctgccg gcggcccgac gatcattacg caagtcgtcc aggcgctggt
    11101 gcggtcgctc gacctgggcc tgccactgga ccaggccctg gcccagccgc gggttcacca
    11161 ccagtggtcg ccgggcgaag tgcggcacga atcggggttg ccggccgaat tgcaaaattc
    11221 gctggccggc cggggacacc aattgaagag cgtgcgcaat atgggcgtca cgcaggcgat
    11281 cgtattcgac aaggccagcg gacagttcat cggcgtgcac gacccgcgcg tgccgggcaa
    11341 ggccgctggg ccgtagggga gggcggagtg acggagagac ggagggacag agagacaggg
    11401 agaaggagtg acagagtgac ctcgttcgct ccgccaatcc ttctctctgt cgctttgtca
    11461 ctcattccgg ccggcacaaa ggcggtcgcg caaaccgacg acgcacccgg tcgctggaag
    11521 ctggagaccc tcacgctggc cgagggcaaa cagtaccgcg gagtcgtgct cgaggaaagc
    11581 cccgaaagca ttgagttcgc cgaaatcgtg cagccggcgg gaaagccgat gcacgcggtg
    11641 atccgcggcg tcccgcggcc gatggcgagc aaactccagc ggctcgaggg cccggagcac
    11701 caggagctcg tcgcgcgctt cgctcgcttc cgccaccggg ccgtcatcga agccggccgg
    11761 ctggaagaag tcgagctgga agccaacgat tccggcggcg cggcggtgct gctttacgac
    11821 ggcccctggt tcacgctgac cagcaccgcc
//
LOCUS       DASRJB010000358        16292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1512070, whole genome shotgun sequence.
ACCESSION   DASRJB010000358 DASRJB010000000
VERSION     DASRJB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512070"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFB80_09420"
     CDS             <1..721
                     /locus_tag="VFB80_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34027.1"
                     /translation="YAKFCRNLVNWLTESSMIGRRRLVATTDKRFYRPGETIAIQAAT
                     YDESAAPTKNYRVRGMVEPHTPPGEPEPETSPLKWPAGLAREGGATGPHVEWGEPFDL
                     ALGGGGNAEKPQHIIQLPLADVLSSGTGSQSLRVELTAYEDQTQVDSTSLDIQVLHDP
                     FEQQNPFPNHDLMLRLAGASGGKVIRTPAELAAVLRDVPLDVGPPIVRRSPLWSTWWL
                     WGLLAGLLTIEWCWRRSIGLA"
     gene            743..1591
                     /locus_tag="VFB80_09425"
     CDS             743..1591
                     /locus_tag="VFB80_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34028.1"
                     /translation="MAKRSAWVLILVAVSAIAASAQQPAEENKPGGSALEQAAAVARR
                     LEAWRPGKSGERGVVPAIERPLLLFGDDPRNHTRGILWGWGKGRPVAVAELWRGRAEN
                     APYAASLTVAGLEKIEITADGARKWQPAETQVAFAPVAGASAPDAKEPGRLRQIKEIA
                     RRFTAHEFWDPDNSRFELRLLVQPVHRYRDSDAEIQDGALFVLAHGTNPEALLLIEVV
                     GPAIEKSRWQYGLVRTSNAEIHVELDGKEVWTQPRVKGSALRPEHPYYVFTLPAAAAP
                     APTGNP"
     gene            1674..2093
                     /locus_tag="VFB80_09430"
     CDS             1674..2093
                     /locus_tag="VFB80_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN34029.1"
                     /translation="MPRIPQDVTDAELALLQLLWDHGPSSVRRLAERMYDKSSASQHA
                     TVQKLLERLEAKGCVERDRGSWPHTFAAAIERGELIDRKLQQTADKLCDGSIQPLLTH
                     LVKGARLSADQRQSLRDLLDDLEQEKSDKEGRRGGKK"
     gene            2153..4180
                     /locus_tag="VFB80_09435"
     CDS             2153..4180
                     /locus_tag="VFB80_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN34030.1"
                     /translation="MTWLVTTGLTNALVAVLLAGTAYGLGRWLKRPALTHVLWVLVLI
                     KLLTPPVFPVPIGWRIDPAIFGELAPVAAVEPMSVAENCPAPSGVGVACEAPAAGPAP
                     IAAASIAPVKAAPARRLVSAAPPAWLAILAAPANWLRAAVAVWFIGSAAIALLFLWRT
                     RRFQQYLRLAARNDAELDARLSELARSARLDSRPHIVTVESAISPMLFGLGRSAVLIF
                     PAALNRQLAPAARDTLLLHELAHYARGDHWVRLVELLAQVVFWWHPVVWWARRELEAA
                     EEECCDAWVVERHAGVPRLYAEALLATIDFLCEAPAPLPPAACGLGDVPLLRARLTQI
                     IRGRLPEQLCPATKTAVFLAAMFVLPVFPALFDSAVAAAPAAVITAAVETVTPEFTPV
                     TLDPAVELTSVPPVDEPAGGMNATMAEVLKYVRPPRRAKLYATAVSPDGRYKVEIREG
                     YQATLFQGLIPNRLIRDPINLAEPLAKCVAFSPNSLSLAAGNDNGHVSLYDCSSGGLS
                     KRLKGATAPVRTVAFSPDSEGRQVAAGAADGSVIVWDVATGDFRILLAPGPVPAICVR
                     WSSDGRWLAVSLGDDLAEPDAATLVICSTEKGEIRGQFALPAAAGAIEWQDDDRTILS
                     ADYGGEYTIRQIADGQVLSQGQMDRTRVSAAQLSPNATPGIAEQLVARAAP"
     gene            4177..5013
                     /locus_tag="VFB80_09440"
     CDS             4177..5013
                     /locus_tag="VFB80_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34031.1"
                     /translation="MIGRWCALALTGAILLFSILAAQEPGGAGENKAQIEAALKLTKE
                     AAAKYEFAIGGENSVRPELLAEPVLRWSNPAVGEIHGNVFLWTIGGRPAVISSIFKWF
                     SPHTHMSHEFHSLSDQPLRGKFEGNEVWVAAEPGLKFAPLPDSPAPAASKSQRLLQMR
                     ELAKSCASTKRERDGNVTELRLLTQPIYRYEAPQAGLIDGALFTFVQGTDPELFLLLE
                     AREAREAGGRQEWQFAVTRMNSVAFDLRYKDREIWKVDVMPWSDVGQHRKPYTSFMHR
                     MP"
     gene            5291..6118
                     /locus_tag="VFB80_09445"
     CDS             5291..6118
                     /locus_tag="VFB80_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34032.1"
                     /translation="MLTLLLLQMLAAADEPASDDQQRREAVARLAPAKAKQMNVLVGD
                     QEPVQAALVEKPLLRWSNPTAGSVFGEVYLWTSRERPVAIASIYRWYHPFKDSTVEVV
                     SLSSRPVQAREGEAALWQSPADGVTWQKLASAPRPAETANARLGQMRSLAREFSAELA
                     DQRSGEQVARELRLLNQPVHRYASPESGVIDGALFAFVETTDPEAWLLLEAAAQEGQT
                     GWRFALARMNADELRIKRGGELAAGWSKIVQPWRYRSAPYTLFGFDPDQVKIEPERN"
     gene            6120..6938
                     /locus_tag="VFB80_09450"
     CDS             6120..6938
                     /locus_tag="VFB80_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34033.1"
                     /translation="MGTPRLGLAMLLVAAGATLWAQDGPGPKTLREMIDASVKQVEIL
                     AGSEAALPAEPLVALRWANNARGSEDGTTLLYIHEGKPLATACLYPWDGKLVHDFESL
                     SRGPLIGRKDGAVIWRPKTGLKFAPIPDALPPEATPAQRLRQMKTLSEQFQSSLLGWK
                     ADNTDREQLRLLPKPLYRYEPKSGPVFDGAVFAFVMGTDPESLLLIEAVETGGRRTWE
                     FAFARRTSGELEGRHKDKVVWHAQRFPGMKNPDHPRISVGTPIPPKLLAPAQEK"
     gene            6942..7778
                     /locus_tag="VFB80_09455"
     CDS             6942..7778
                     /locus_tag="VFB80_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34034.1"
                     /translation="MSSAALLALLLALAADPPATFEQRLREINVQEAKTWELFLDAEH
                     QVKAEFLERPIYLWSNPTKGAGQYGSVFLWVHDGRPAAVGSFFGHPFRGDERRMVHEF
                     HALARAPLHPVCTDGDAKTWTPKAGITLGPLPESPPPEPSSTKRLLQMRNLGRQFGGY
                     MVNFRQQRWELRLLPQPLYRYDKPPEGVIDGALLALVSDAGTDPEVLLLIEAREPSGW
                     QYALLRFSDSSAYVTHQGKEIWTAVKDNPNFAAYNADHTYRVLQKRLLTEPAAQPDKP
                     AP"
     gene            7775..8710
                     /locus_tag="VFB80_09460"
     CDS             7775..8710
                     /locus_tag="VFB80_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34035.1"
                     /translation="MIKHFLVGLALSAAWAAQAAGQDAANAAPGTRRALVICGLPGDP
                     PHRQLFAESTEKLHAGLIQNHGFAAENIAVVWGETPTDKDGPAVKSSRGPATREALAQ
                     AAADLEKVLKPEDTLWLFVLGHAHYDGRFSWLNLPGPDIQQVEFGKLLAGVRCREQVF
                     FITTSASGFFLKPLAQSGRIVIAATEPDLEVNETVFPHKLAAALSAPPGYEEFDVDRD
                     GRMTLLDLYLWTARATAQDYAAGELLATEHAQLDDTGDGRGTELQAAFLPEDLGGRLQ
                     AGKDPPLITAGEGAAARLIWLPSPPAPPQPPRTAD"
     gene            complement(8707..10584)
                     /locus_tag="VFB80_09465"
     CDS             complement(8707..10584)
                     /locus_tag="VFB80_09465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN34036.1"
                     /translation="MTLWSTEHLVVTLSGPEGRREFVVPRPFARIGAHPASEIVLSGP
                     GIAKRSHYLHATGDGLYCLDLDVEDADAENRGRWLAADDVLQVGPYQLTVRCASASQD
                     RLQLPPPIARGSAAAPLPVVNVHCQRLLKDKRRFRARLNLVGRRPQCALQLRGSRVSA
                     FHCALYWDQRRLWCVDLLSSNGTQLNGEKIDFAEMQLNDRLDVGEFSLVYFRWSPRSS
                     MQPGWQPDAGGQEHDLADADDSAPAFDALSPPPTADASSSAAPEPALLREQLAGLRAA
                     DASWEQERHALESARLQAENERRTLSDQLAAAAQRSDALTAELAAAAAALAARQTEVE
                     TLAQRLAASEQAFAASQSKLEIVAQEKAAALARAEGLLAALPQQEQRSLQLAAEVARL
                     AQERSDLDARLNNQIAELARERSAAQRARDEWQAEHVQLARQLAAESRSTADLQAALA
                     QREAELTQRQAEADAARNDWQAERQALSDELAERGRQLAALQAELAAAAAQVAARQEE
                     VARQVAPLESAESTAAAGEAEPLPLVEFSPAPEADDSPALDVELETPAESVPLVVRQD
                     APAGRRSKAQRQELTTFVGDRLIELDSSHRRRRWLLWGSLAAGTIALSAAILSVLYLL
                     R"
     gene            10895..11452
                     /locus_tag="VFB80_09470"
     CDS             10895..11452
                     /locus_tag="VFB80_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HZN34037.1"
                     /translation="MPILASETHLHPAQLLDGISDPADAPRKWWAVYTKSRQEKSLAR
                     DLLSQDIPFYLPLVPKVSVIAGRRVKSELPLFGSYLFLFATDDERVLTLATKRVAHLV
                     SAPDATEITRALRQIQQLIAAGVPMTVEGRLCAGRRVRIKHGSLMGLEGVVVARRGED
                     RLLVTVDFLQQGVSILISDYQVEPV"
     gene            complement(11467..11931)
                     /locus_tag="VFB80_09475"
     CDS             complement(11467..11931)
                     /locus_tag="VFB80_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34038.1"
                     /translation="MWLAALLPLLAAGCCAGYHNHSGQAASCEAGPGWYETPLCFGFY
                     STCWRPWPEECPSCPSFALPQAAEQVLPGPAAPAPAQPMAPMPELTPPQPSPPMPPAA
                     PPMPMPEPAQPPAGAAYELPPGDEAFLRRRWVEGGFVAQPASANLPVELPSP"
     gene            complement(12023..13087)
                     /locus_tag="VFB80_09480"
     CDS             complement(12023..13087)
                     /locus_tag="VFB80_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZN34039.1"
                     /translation="MPKFRTLLVVVLAAALAGCKATHHAAAPPAGWEFLPPPDMPREL
                     SKVVLPTYTIEPPDILVIEAIHLVPRSPYLLRTGDVVAINVVGALPDAPITGAFPIQP
                     GGLVNLGIPYGSVKLTGLNTDQALEALKAHLAMHVAGAVVSLSLVEMSGKQQIAGQHL
                     VGPDGTVTLGSYGSVQVVGLTLAQAKQAIEQHLARSLEDPEVAVDVFAYNSKVYYVIT
                     EGAGLGDNVTKFPITGNDTVLDAIANINGLTEVSSKKIWIARPVPHFGQMQILPVDWR
                     AMTASGAAVTNYQIMPGDRVFVAENGLVAFDSHLAKLLAPFERAMGFTLLTAGTATRL
                     SGPVLRGGGNPQNFGGGGFQ"
     gene            13404..13652
                     /locus_tag="VFB80_09485"
     CDS             13404..13652
                     /locus_tag="VFB80_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34040.1"
                     /translation="MEKTAFLARRLHQDESREGWETTVRASDLAVGQRVRVTSGPLNG
                     LTGVVAGQAANGNWVVDIAESARGVLLCIRAHQLARAD"
     gene            14314..14664
                     /locus_tag="VFB80_09490"
     CDS             14314..14664
                     /locus_tag="VFB80_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34041.1"
                     /translation="MKRAAILAAAIGAACLVGEASAQTPRYVPPAGRTLPEELNYFRR
                     DTGVLDQYNGFIAPLRQLDNQLRTMQQQQQTDFQSAQRAISQVRTSQAAPTGAGAGFM
                     NYSHYYRMPGAYRR"
     gene            14793..14990
                     /locus_tag="VFB80_09495"
     CDS             14793..14990
                     /locus_tag="VFB80_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34042.1"
                     /translation="MAHEIAKLLLEHAETFLERTEAVKSALSLGMPLNEIQDYLDWLD
                     ALRSRDQPTSKDRAGDPPQKP"
     gene            15592..>16292
                     /locus_tag="VFB80_09500"
     CDS             15592..>16292
                     /locus_tag="VFB80_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HZN34043.1"
                     /translation="MPARPLHSRLPKRRRLLEKLEPRHLLTAAPAAAGDDLSALLSRG
                     TVCSCPICSGVGLDQIPVMESGGSGPAGASPLSSLPQLHSNAGATARLFLDFNGHFQA
                     TWGDWNNATTPVYDQDGDATTFSNGELASIVEIWTRVAEDFAPFNIDVTTVDPGGQAN
                     QVVARIAIGGNWSDWYGSTSGGVAYVGGFYNPAPNVGYVFENALGNGNAKFVAEAATH
                     EAGHLFGLQHQALWSG"
BASE COUNT         2793 a         5340 c         5353 g         2806 t
ORIGIN      
        1 ttacgccaag ttctgccgca acctcgtgaa ctggctgaca gagagctcga tgatcggccg
       61 ccgccgcctg gtggcgacga ccgacaagcg gttctaccgg ccgggcgaga cgattgcgat
      121 tcaagccgcg acctacgacg aaagcgcggc cccgaccaag aactaccgcg tccggggaat
      181 ggtcgagccg cacacgccgc cgggcgagcc cgagcccgag acttcgccgc tcaaatggcc
      241 ggcgggcctg gcccgggaag gcggcgcgac cgggccgcat gtggaatggg gcgagccttt
      301 tgatttggcg ctgggcggcg gcggaaacgc cgagaagccg cagcacatca tccagctgcc
      361 gctggccgac gtgctctcca gcggcacggg cagccaatcg ctgcgcgtcg agttgacggc
      421 ctatgaagat caaacgcagg tggacagcac ctcgctcgac atccaggtgc tgcacgatcc
      481 gttcgagcag cagaatccgt ttcccaacca cgacctgatg ctgcggctgg ccggagcgtc
      541 gggcggcaag gtgattcgca cgcctgccga gctggccgcg gtgctccgcg atgtgccgct
      601 cgacgtgggc ccgcccatcg tccgccgctc gccgctgtgg agcacgtggt ggctgtgggg
      661 gctgcttgcc gggctgctga ccatcgagtg gtgctggcgc agatcgatcg gcctggcttg
      721 aacgcgcggg agggcaacga acatggccaa acgttctgct tgggtcctga ttctggtggc
      781 cgtcagcgcg attgccgcca gcgcgcagca gccggccgag gagaacaagc ccggcggctc
      841 ggcgctggag caagccgcgg ccgttgccag gcgactcgaa gcgtggcgtc cggggaaatc
      901 tggcgaacgg ggcgtcgtgc cggcgatcga gcggccactc ctcttgttcg gcgacgatcc
      961 acgcaaccac actcgcggaa ttctctgggg ctggggaaaa ggccggccgg tcgccgtggc
     1021 cgagctgtgg cgcggcaggg ctgagaatgc gccctatgcc gcgtcgctca ccgtggccgg
     1081 actggagaag atcgagatca cggccgacgg ggcgcgcaag tggcagcccg cagaaacgca
     1141 ggtcgccttc gcgcctgtcg ccggagcttc cgcgcccgac gccaaagagc cgggccgact
     1201 gcgccaaatc aaggaaatcg cccgccggtt caccgcccac gaattctggg acccggacaa
     1261 ctcgcggttc gagctccggc tgctcgtcca gccggtgcat cgctaccggg actcggacgc
     1321 tgaaatccag gacggggcgc tgttcgtgct ggctcacggc acgaatcccg aagccctgct
     1381 gctgatcgaa gtggtcgggc cggcgatcga aaagtcccgc tggcaatacg gcctggtgcg
     1441 gacgagcaac gccgaaatcc acgtcgagct ggacgggaaa gaagtctgga cgcagccccg
     1501 ggtcaaaggc agcgccctgc ggcccgagca cccgtattac gtgtttactc tgccggcggc
     1561 ggccgcgccc gcgccgaccg gcaatccctg aaaaaattcc cgcacgtgtt gcacttcaga
     1621 cgctttgtct atatcatgcc agctgacgat gcatgtcagg aaaggccagg gaaatgcccc
     1681 gcataccaca agatgtgacc gacgccgaat tggcgctgct ccagttgctc tgggaccatg
     1741 gaccctcgtc cgtgcgccgc ctggcggagc gaatgtacga caaatcgtcg gcctcgcagc
     1801 acgccaccgt gcagaagctg ctggagcgct tggaggccaa gggctgcgtg gagcgcgacc
     1861 gcggctcctg gccgcacacg ttcgcggcgg ccatcgagcg cggcgagctg atcgaccgca
     1921 agctccagca aacggccgac aagctctgcg acggctcgat tcagccgctg ctgacgcacc
     1981 tggtcaaggg ggccaggctc tccgcggatc agcggcagtc gctgcgcgat ttgctggacg
     2041 acctggaaca ggaaaaatct gacaaagaag gccgccgggg cggaaagaaa tgaccgcagg
     2101 cggcgaatag gcagcggaac atcctggtgc gcgccatcca gggagcagag ccatgacctg
     2161 gcttgtcacg accggcctga cgaatgcgtt ggtggcggtg ctcttggccg ggaccgccta
     2221 tggccttggc cgctggctca agcgtccggc attgacgcat gtgctgtggg tcctggtgct
     2281 catcaagctg ctcacgcccc cggtgtttcc cgtgccgatc ggctggcgca tcgatccggc
     2341 gattttcggc gaattagcgc cggtcgccgc ggttgagccg atgtctgtgg cggagaactg
     2401 tcccgcccca agcggagtcg gcgtagcgtg tgaggcgccc gctgcgggtc cggcgccgat
     2461 cgcggccgcc tccattgccc cggtcaaggc cgcgcctgcc cgccggttag tatcggcagc
     2521 ccctcccgcc tggttggcga tcctggctgc gccggcgaac tggctgcggg cggcggtggc
     2581 cgtttggttc atcggctccg ccgcgatcgc cctgctgttc ctctggcgga cgcggcgatt
     2641 ccagcagtat ttgcggctgg ccgcccggaa cgatgccgag ctcgatgcac gcctgagcga
     2701 gctggcgcgc agcgcccggc tggattcgcg tccacacatc gtgaccgtcg agagcgcgat
     2761 ctcgccgatg ctgttcggcc tgggccgcag cgccgtcctg attttcccag ccgcgctcaa
     2821 ccggcagctc gctccggccg cccgcgacac actgctcctg catgagctgg ctcactatgc
     2881 ccgcggcgac cactgggtgc ggctggtcga attgctcgcg caagtcgtct tctggtggca
     2941 cccggttgtc tggtgggcgc ggcgcgagct ggaagccgcc gaggaagaat gctgcgacgc
     3001 gtgggtcgtg gagcggcatg cgggggttcc gcggctgtat gccgaggcgc tgttggcgac
     3061 gatcgacttt ttgtgcgaag cgcccgcgcc tttgccgcca gccgcctgcg gcctgggcga
     3121 tgtgccgctg ctcagggccc gcctgacgca gatcattcgc ggtcggctgc cggagcagct
     3181 ttgcccggca acgaagaccg ccgtgtttct ggcggccatg ttcgtcctgc cggtctttcc
     3241 cgcgctgttc gattccgcgg tcgccgccgc accggcggct gtgatcacgg ccgcagtcga
     3301 aaccgttaca ccggagttca ctcccgtcac gctggatccg gcggtggagc ttacctctgt
     3361 tccaccggtc gatgagccgg ccggcggcat gaacgcgacc atggcggaag tgctgaagta
     3421 tgttcggcca ccacgccggg caaagctcta cgccacggcg gtatcacccg acgggcgcta
     3481 taaagtggaa atccgcgaag gatatcaggc gactttgttc caaggtttga ttccaaaccg
     3541 gctcatccga gatccgatca atctggccga accgctggcc aaatgcgttg cattctcgcc
     3601 caacagcctg tcgctggccg cgggcaacga taatggccac gttagcctgt acgactgctc
     3661 gtcgggcggc ctaagcaaga ggctcaaggg tgcgacggcg ccggttcgca cggtagcctt
     3721 cagccccgac agcgagggcc ggcaagtggc cgcgggcgca gccgacggca gcgtgatcgt
     3781 gtgggacgtg gccacgggcg acttccggat cctgctcgcg cccggcccgg tgcccgcgat
     3841 ctgcgtgcgc tggtccagcg acggccgctg gctggcggtc agcctgggcg acgatctggc
     3901 cgagccggac gcggccaccc tggtgatttg ctcgacggag aagggcgaga tccgaggcca
     3961 gttcgcgctg ccggcagccg ccggcgcgat cgagtggcag gacgacgacc ggactatctt
     4021 gagcgccgac tatggcgggg agtacacgat tcgccaaatt gcggatggac aggttctttc
     4081 ccaaggacaa atggatcgca cgcgggtctc ggcggcccag ttgtcgccga atgcaactcc
     4141 cggcattgcc gagcaactcg tagcgcgagc agcgccatga tcggccgctg gtgcgccctg
     4201 gctttgacgg gggctatact gcttttcagc attctggcgg cgcaggagcc tggcggcgcg
     4261 ggcgagaata aggctcagat cgaagcggcc ctgaagctga ccaaagaggc cgcggcaaag
     4321 tacgagttcg ccattggcgg cgagaattcg gtgcggccgg aattgctcgc cgaacctgtg
     4381 cttcgctggt cgaatcccgc ggtaggcgag attcacggca acgtcttcct gtggacgatc
     4441 ggcggccggc ccgccgtgat cagctcgatc ttcaagtggt tctcgccgca cacgcacatg
     4501 tcgcacgagt tccactcgct cagcgaccag ccgctccgcg gcaagttcga ggggaacgaa
     4561 gtctgggtgg cagctgagcc agggctcaaa ttcgccccgc tgcctgattc accggccccg
     4621 gccgcaagca agtcgcagcg cctgctgcaa atgcgcgaac tggccaagtc ctgtgcctcg
     4681 accaagcgcg agcgggacgg caacgtgacc gagctgcgcc tgctcacgca gcccatctat
     4741 cgctacgaag cgccgcaggc gggcctgatc gacggagcgc tattcacgtt cgtccaaggc
     4801 accgaccccg agttgttcct gctgctggag gcccgcgagg cccgcgaggc cggcggcagg
     4861 caggagtggc agtttgcggt cacccggatg aacagcgtcg ccttcgacct gcggtacaag
     4921 gaccgggaaa tctggaaagt ggacgtgatg ccctggtctg acgtcggcca gcaccggaag
     4981 ccgtacacgt cgtttatgca ccgcatgccg taaccgacgc cgaccggaaa tgtgaacatt
     5041 ggtatggtat ttgcaccaaa gcttcgtttg cgccgcggcc tgccagtttg gcactggcgg
     5101 cggcggccgg cgaggacacc gaaggctctc tcaaacgcta ataaagtagt acttatcgtg
     5161 cggcggatga ttcgggcgca agtccatgag ccgcctgacg ttgaggcaaa aaatggagcg
     5221 aagagggcga ttcgctgtgg tccctccggg tgggatgcaa acgggtgggc gtgaccgcgc
     5281 ctaaggaaac atgttgacgt tgcttcttct ccagatgctg gccgcggccg acgagccggc
     5341 gtccgacgat caacaacggc gggaagcggt ggcgcggctc gcgccggcca aggcgaagca
     5401 gatgaacgtg ctggtgggcg accaggaacc ggtccaggcc gccctggtcg agaagccgct
     5461 cttgcggtgg tcgaatccga cggccggcag cgtgttcggc gaggtttatc tgtggactag
     5521 ccgggagcga ccggtcgcca ttgcctcgat ctatcgctgg taccatccgt tcaaggactc
     5581 gaccgtcgag gtcgtctcgc tgagcagccg accggtgcaa gctcgcgagg gcgaggcggc
     5641 gttatggcag tcgcccgccg acggcgtgac ctggcagaaa cttgccagtg cgccccgtcc
     5701 agcggaaact gccaacgccc gcctcggcca gatgcgctcc ctcgcccgcg aattctccgc
     5761 ggagctggcc gaccagcgga gcggcgagca ggtcgcccgc gaactgcggc tcttgaatca
     5821 gcccgtgcac cgctacgcca gcccggagtc gggcgtcatc gacggagccc tgttcgcctt
     5881 cgtcgagacg accgatcccg aagcctggct gctcttggaa gcagctgccc aggaaggcca
     5941 gaccggatgg cgttttgccc tggcccgcat gaacgccgat gaactgcgga tcaagcgggg
     6001 cggcgagctg gcggccggct ggtccaaaat cgtccagccc tggcgctacc gctcggctcc
     6061 gtacaccctg ttcggcttcg acccggacca ggtcaagatc gaacccgaga ggaattgaca
     6121 tgggcactcc acggctcggg ttggcgatgt tgctggtcgc ggccggcgcg acgctctggg
     6181 cacaagacgg ccccggtccc aagaccttgc gcgagatgat cgacgcctcg gtcaagcaag
     6241 tcgaaatcct ggctggcagt gaggccgcgc tgccagccga gccgctcgtt gctctgcgct
     6301 gggccaacaa cgcccgcggc agcgaagacg gcaccaccct gctctacatc cacgagggca
     6361 agccgctggc gaccgcctgc ctctatccct gggacggcaa gctcgtgcac gacttcgaaa
     6421 gcctcagccg cgggccgctg atcggccgca aggacggcgc cgtgatctgg cgaccgaaga
     6481 cggggctcaa gttcgccccg attcccgatg ctctgccgcc ggaggccacg ccggcccaac
     6541 gcctgcggca gatgaagacc ctctccgagc aattccaatc gagcttgctc ggctggaagg
     6601 cggacaacac cgaccgcgag caactgcggc tgctgcccaa gccgctgtat cgctacgagc
     6661 cgaagagcgg accggtgttc gacggcgcgg tgttcgcctt cgtgatgggc accgacccgg
     6721 aatcgctgct gctgatcgaa gcggtcgaga cgggcggcag gcgcacctgg gaatttgcgt
     6781 tcgcccgccg cacgtcgggc gaattggaag gccgccacaa ggacaaggta gtgtggcacg
     6841 cccagcgctt tccggggatg aagaatcccg atcacccgcg gatctcggtc ggcacgccga
     6901 ttccgccgaa gctcctcgcg ccggcgcagg agaaatagcc gatgtcgtcc gcagccctgc
     6961 tggccttgct cctggcgctg gccgccgatc cacccgcgac gttcgagcag cggctgcgcg
     7021 agatcaacgt gcaagaggcc aagacgtggg agttgtttct cgacgccgag caccaggtca
     7081 aggccgagtt tctggagcgg ccgatatacc tgtggtcgaa tccgacgaag ggagctggcc
     7141 agtacggctc ggtttttctg tgggtgcatg acgggcggcc ggcggcggtg ggcagcttct
     7201 tcggacaccc gttccgcggg gacgagcggc ggatggtcca cgaatttcat gcgctggcca
     7261 gggcgccgct ccatccggta tgcactgacg gcgacgcgaa aacgtggacc cccaaagccg
     7321 ggatcacgct cggcccgctt cccgagtccc cgccgccgga accgtcgtcc acgaagcggc
     7381 tgctgcaaat gcgcaacctc ggcaggcagt tcggcggcta catggttaat ttccgccaac
     7441 agcggtggga gctgcggttg ctgccgcagc cgctctatcg ctatgacaag ccgccggagg
     7501 gcgtgatcga cggggcgctg ttggccctgg taagcgacgc cggcaccgat ccggaagtgc
     7561 tgctcttgat cgaggcccgc gagcccagcg gctggcagta cgcgctgttg cggttttcgg
     7621 attcgagcgc ctacgtcacg caccaaggca aggagatttg gaccgcggtc aaggacaatc
     7681 caaatttcgc cgcttacaac gccgaccata cgtatcgcgt tcttcagaag cgccttttga
     7741 ctgagccggc agcccagccg gacaagccag caccatgatc aagcatttcc ttgttggatt
     7801 agcactgtcc gcggcgtggg ccgcgcaggc cgcgggtcag gatgcagcga atgctgcccc
     7861 cggcacgcgc cgcgcgctcg tcatttgcgg tcttcccggt gacccgccgc accgccaact
     7921 gttcgccgaa tcgacggaga agctgcacgc ggggctgatc cagaatcacg gttttgcggc
     7981 cgagaacatc gccgtcgtct ggggcgagac gccgaccgac aaggacggcc cggcggtaaa
     8041 gtcgagccgc ggtcccgcca cgcgcgaagc gctcgcgcag gcggcggccg acctggagaa
     8101 ggtcctcaag ccggaggaca cgctgtggct gttcgtcttg ggccacgcgc actacgacgg
     8161 ccggttcagc tggctgaact tgcccgggcc ggacatccag caagtcgagt tcggcaagtt
     8221 gctggccggc gttcgctgcc gcgagcaagt gttcttcatc acaacctccg cgagtgggtt
     8281 ctttctgaag ccgctggccc agagcggccg gatcgtgatc gcggcgacgg agccggacct
     8341 ggaagtcaac gaaacggtgt ttccacataa gttggcggcg gcgctttccg cgccgccggg
     8401 ctacgaagag ttcgacgtcg atcgcgacgg gcggatgacg ctgctcgacc tgtatttgtg
     8461 gaccgcgcgg gcgaccgccc aggactatgc cgccggcgag ctgctggcga ccgagcacgc
     8521 ccagctcgac gacaccggcg acggccgcgg cacggagttg caggcggcgt tcctgccgga
     8581 ggatctgggc ggccgactgc aggccggcaa ggatccaccg ctcatcaccg ccggcgaggg
     8641 agcggcggcc cgcttgatct ggctgccctc tccgcctgcg ccgccgcagc cgccgcgcac
     8701 cgcggactag cgcagcagat agagcacgct gaggatcgca gcgctgagtg caatcgtccc
     8761 cgctgccagc gatccccaga ggagccaacg gcggcggcgg tgcgagctgt ccagctcgat
     8821 tagccggtcg ccgacgaacg tagtcaactc ctggcgctgg gccttgctgc ggcgtcccgc
     8881 cggcgcgtcc tgccgcacca cgagcggaac cgactcggcg ggagtttcca gctccacgtc
     8941 gagcgccggc gagtcgtccg cctcaggcgc tggggagaac tccacgagcg gaagcggctc
     9001 cgcctcgccg gccgctgcag tgctttccgc cgattcgagc ggcgcgacct gacgggcgac
     9061 ctcctcctgg cgggcggcga cctgcgcggc agcggccgcc aattcggctt gcaaggccgc
     9121 caattgccgg ccgcgctccg cgagctcgtc ggatagcgct tgacgctccg cctgccagtc
     9181 gttgcgtgcg gcatcggctt ccgcctgccg ttgcgtaagt tccgcctcac gctgagccag
     9241 cgcggcctgc aagtcggcgg tgctgcgcga ctcagcggcc agctgccggg cgagctgtac
     9301 gtgctccgct tgccactcgt cccgcgcccg ttgagccgcg ctccgctccc gcgccaactc
     9361 cgcaatctgg ttgttcagcc gggcatccag gtcgctgcgc tcctgagcca ggcgggccac
     9421 ttcggccgcg agctgaagcg agcgctgctc ttgctgcggc agggcggcaa gcaggccttc
     9481 cgcccgcgct agggcggcgg ccttttcctg tgcgacgatt tccaatttcg actggctcgc
     9541 ggcgaacgct tgctcgcttg ccgccagccg ctgcgccagt gtttcgacct cggtctgccg
     9601 cgcggccagc gccgccgccg cggcggccag ctcggcggtg agcgcgtcgc tgcgctgcgc
     9661 ggcggcagcc aactgatcgc tcagcgttcg gcgctcgttc tcagcctgca gacgtgccga
     9721 ctccagcgcg tgccgctcct gttcccagct cgcatccgca gcgcgcagtc cggccaattg
     9781 ctcgcgcagt agcgcgggct ccggcgctgc actcgaactc gcgtcggccg ttggcggggg
     9841 cgaaagggcg tcgaaagccg gcgctgaatc gtcggcgtcc gccaggtcgt gctcctgtcc
     9901 gccggcgtcc ggctgccagc cgggctgcat gctgctgcga ggcgaccagc ggaaatagac
     9961 caggctgaat tcgccgacgt cgagccggtc gttgagctgc atctcggcaa aatcgatctt
    10021 ctcgccgttg agctgcgttc cgttgctgct gagcaaatcg acgcaccaca gccgccgctg
    10081 gtcccagtac agagcgcaat gaaaggcgga gacgcgcgag ccgcgcagct gcagggcgca
    10141 ttgcggccgc cggcccacga gattcaaccg cgcccgaaaa cgtcgcttgt ccttcagcag
    10201 gcgctggcaa tgcacattga cgaccggcag cggcgcggcg gcgctgccgc gggcgatcgg
    10261 cggcggcagc tgcagcctgt cctggctcgc ggaagcacaa cgtacagtca gctgatacgg
    10321 accgacttgc agcacgtcgt cggcggcgag ccagcggccg cgattctcgg cgtcagcatc
    10381 ctcgacgtcg aggtccaggc aatacagccc gtcgccggtg gcgtgcagat agtgcgaacg
    10441 cttcgcgatg cccggaccgg aaagtacgat ttcggacgcg ggatgcgcgc cgatgcgagc
    10501 aaacggccgc ggaacgacaa attcgcggcg gccttccggt ccggaaagtg taacgaccag
    10561 atgttcggtg gaccacaacg tcatggcgag gcgagaaaaa ctcgtgattc tgcagtctag
    10621 atacgatcgg cggcgaatgc atcgaacgag cgcccgaaat cgcggaaaga ttcttgcagc
    10681 cgcaagacaa ctatgttgat gaattaccga accaaactcg ttgacggacg tctacaccgt
    10741 ttgtacagtc aatcatccgc gccgctcgct actcttcgcg agctgcgttg gagcctaccg
    10801 acaacttcct gcctggcgcg cttggctggt tcccgtcgct tcacccgtag tgcgaactgg
    10861 gatcaacttt tgcgctcctg gagtggttca cccgatgcca atccttgctt cggaaactca
    10921 tctgcacccg gcccaattgc tcgacgggat cagtgaccca gccgacgcgc cgcgcaagtg
    10981 gtgggctgtt tacacgaagt cgcgccagga gaagtcgctg gcccgcgact tgctctccca
    11041 ggacattccc ttttacttgc cgctggtgcc caaggtcagc gtcatcgccg ggcggcgggt
    11101 caaatcggag ctgccgctgt tcggcagcta tctgttcctc ttcgcgaccg acgatgagcg
    11161 cgtgttgacg ctggcgacga agcgcgtcgc ccatctggtt tcagctccag acgcaaccga
    11221 aatcactcgc gccttgcgcc aaatccaaca gctgatcgca gccggagtgc cgatgacggt
    11281 ggaaggccgg ctgtgtgcag gccggcgggt ccgtatcaag catggctcgc tgatgggcct
    11341 ggagggcgtc gtcgtcgccc gccgcggcga agatcgcctg ctggtcacgg tcgattttct
    11401 ccagcagggc gtctcgatct tgatcagcga ttatcaagtc gagccggtct aaaacgcccc
    11461 gcatcttcag gggctgggca attccacggg cagattcgcc gaggcaggct gcgcgacaaa
    11521 gcccccttcg acccaccgcc ggcgcagaaa tgcttcgtcg cccggcggca gctcatacgc
    11581 ggcgccagcc ggcggctgag caggctcggg catcggcatt ggtggcgcag ccggcggcat
    11641 cggcggcgag ggctgcggcg gagtcagctc gggcatcggc gccatcggct gcgcaggcgc
    11701 aggggccgca ggtccgggca acacctgctc cgccgcctgc ggcaacgcga aggacgggca
    11761 gctcgggcac tcttcgggcc agggacgcca gcaggtgctg tagaagccga agcagagcgg
    11821 cgtctcgtac caaccgggtc ccgcttcgca gctggcagcc tggcccgagt ggttgtgata
    11881 gccggcgcag cagcccgccg cgagcagcgg cagcagcgcg gccaaccaca ttcggctgtc
    11941 aggcaaggcg aaagttctca cggtcgagga atcctgtaac gatctgggtc gcaagcggcg
    12001 ctgcaggcgg agcgccgccg cgctattgga agcctccgcc gccaaaattc tgcgggttgc
    12061 cgccgccgcg gagcacgggc ccggacaaac gagtcgcggt gccggcggtc agcagcgtga
    12121 agcccatcgc ccgctcgaag ggagcgagca gcttggccag gtgcgaatcg aacgccacca
    12181 ggccgttctc cgccacgaac acgcggtcgc ccggcatgat ctggtagttg gtgaccgccg
    12241 caccgctggc cgtcatcgcc cgccaatcga ccggcaggat ctgcatctgc ccgaagtgcg
    12301 gcaccggccg ggcaatccag atcttcttcg acgaaacctc ggtcagcccg ttgatgttgg
    12361 caattgcgtc gagcaccgta tcgttgccag tgatcggaaa tttcgtgacg ttgtcgccca
    12421 ggccggcgcc ttcggtgatc acgtagtaga ccttgctgtt gtaggcgaag acatcgacgg
    12481 cgacctcggg gtcttccagc gatctggcca ggtgctgctc gatcgcctgc ttggcctgcg
    12541 ccaacgtgag gcccaccacc tgcacgctgc catagctgcc gagcgtcacc gtgccgtcgg
    12601 gccccaccag gtgctgaccg gcgatttgtt gcttgcccga catctcgacc agcgacagcg
    12661 aaacgacggc gccggccacg tgcatggcca ggtgtgcctt cagcgcttcg agcgcctgat
    12721 cggtgttcaa gccggtgagc ttcaccgacc catagggaat tcccaggttg acgaggccgc
    12781 cgggctgaat cgggaaagcg ccggtgatcg gtgcgtccgg caacgcgccg acgacgttga
    12841 tcgcgacaac gtcgccggtg cgcaacaggt acgggctgcg cgggacgagg tgaatcgctt
    12901 caatgacgag gatgtccggc ggttcgatcg tatacgtcgg caggaccacc ttcgacagct
    12961 cccgaggcat gtccggcggc ggcagaaact cccagccggc cggcggagcg gctgcgtggt
    13021 gcgtcgcctt gcagccggca agcgctgcgg ctaggacgac gacgagcaag gtcctgaatt
    13081 tcggcatgaa cttgcgatcc ccccgctagc accgcgctga cctgcccacg ccaggcaagt
    13141 cgcgatcatt cacaaccact cggctgcgga cgcctgtgaa cttgtatcgg tttttcgcat
    13201 cttgccgttt accccgatta ctcccacttt gagtgcagag cagtctttgc cacctgagta
    13261 caatcgcgcg agccgttcgt tcctgaaaac gaggtcgcga ctattttcta catggaagat
    13321 ggtttttaga atttttggta ggaaaaaacc tccgtggatc agctagctag agatagtctg
    13381 gtcaccttgc ctgaacggat gctatggaaa aaactgcgtt tttggcgcgc cgccttcacc
    13441 aggacgaaag ccgcgagggc tgggaaacga ccgtccgggc gagtgacttg gctgtcggcc
    13501 agcgggtccg ggttacttcg gggccgctga acggattgac gggcgtggtg gccggtcagg
    13561 cggccaatgg caattgggtc gtggacattg ccgaatccgc acgcggcgta ttgctgtgca
    13621 ttcgggctca ccagttggcc cgagcggatt agttcgactg ttcgactcgt tctgttgttt
    13681 gctaggcggc cgctagtgat tgcacaagca actggcgtct agctgcacgg cataatcgca
    13741 gtcgaatcgc tcgccagttt caatcctgcc ggtccactgg ctttctctca agatgacctc
    13801 aaagttaccc gcgggatcca ggattcctac ccgcagagaa gggccgaatt cccccttcgg
    13861 ctcatagtgt cccaccccct tcagcacgct ttttcgtgtc ccaaaggaca cttccagcgc
    13921 gacggtccgc tgatcgaggt gtcgaagttc atggtctgac attgggcaag atccagctac
    13981 gtaatctcct gcggaaattc gcagttaatt attgacggct cctcggcatt ttcaggtctg
    14041 attccttgta ttctgcctat tctaattggt cgctgcgtcg ttttgtcggt tcgcagcaac
    14101 cttggcccaa gaatttaggg attctacaca tgcagacgcg ttccacctag aactttgcgg
    14161 taaagcgcat ttccgcattt tcgccagact ttaggagtcc ggcttcctcc ggcacaatcg
    14221 ttgcaattac accactccca gggctcgcga tttgccgatg gaatcgttac gttgaattat
    14281 tgcaggtgtg ggaaggccgt caggagcact ccgatgaaac gggcagccat tctcgcggcg
    14341 gcaatcggcg cagcgtgcct ggtgggtgag gcatccgccc agactccgcg gtacgtgcca
    14401 cccgctggcc gaacgctgcc cgaagagttg aactatttcc gccgcgacac gggcgtgctc
    14461 gatcaataca acggctttat cgcaccgctg cggcagctcg acaatcagct gcgcacgatg
    14521 cagcagcagc agcagaccga ctttcaatcg gcccagcggg ccatctccca ggtccgcacg
    14581 tcgcaggcgg cgcccacggg agccggggcg ggattcatga actactccca ctactaccgc
    14641 atgccgggcg cctatcggcg ctgaggcagt cctcagtcgc cggcgtcagg ccgcagcgtt
    14701 cgttgcttgc gcggccgcat gtggtatctt tgccatcggt atccagtgcg cgatgggctt
    14761 tgcagctgct tgaatcgcgc gaaggtcgcg gcatggccca tgagatcgct aaactgctac
    14821 tcgagcacgc cgagacgttt ctcgagcgga ctgaggccgt caagtccgca ctttccttgg
    14881 gcatgcccct gaacgaaatc caggactact tggattggct ggacgccttg cgcagccgcg
    14941 atcagcccac gtccaaagac cgtgccggcg atccgcccca gaagccgtga gctgcaccct
    15001 tcgcgctcgc cggagtgctg ttcggcaatg ccgtccaggg cgccgttagg ccggcgggca
    15061 aagcattagg gaaactcggc tcttcttggg aaggcggaaa tcgtccaagt ctgcgcaaag
    15121 ggttggccta tcgaacttta tatccatgaa tggacattag tgacaaaagc actagagacg
    15181 gcccgcggga attatcctta cccccttcgc cgccaggccg cgggccgctt ttctgccgct
    15241 agcgccaatg ggccttccac aaccaacttg tcacagagcg tgcgggccat cctcttatgt
    15301 ttcgatgcgc agtaaggaac atttattccc gcggagccca atttttcctg gatttattcc
    15361 tttgactctc gcggcccgcg ggtccaccgc ctagccgcca ggcgtccgct tgccgtttga
    15421 cagccggcga ttccaaccta gctttgagtt gcgcgtgccg ctctccgcgc gccccgttcg
    15481 gggcctgggc ggcccgtgcg tgggcaggaa tcctgcctcc atcgtccttc gatcccgcct
    15541 caggctcctg agttgatgcg ctccccgcca ctctgggagc ggagtcgcat catgccagcc
    15601 cgtcccctcc attctcggct tcccaaacgc cgacgccttc tggaaaagct cgagccgcgg
    15661 catctgctca cggccgcacc cgcggcagcc ggcgacgatt tatcggcctt gctcagtcgc
    15721 ggcaccgtct gcagctgccc gatctgctcg ggcgttggtc tcgatcaaat tcccgtgatg
    15781 gaaagcggcg gcagcggtcc ggcgggggca agcccgctgt cgagcctgcc acagctgcac
    15841 agcaacgccg gggcgacggc caggctgttc ctcgacttca acggccattt tcaggcgacg
    15901 tggggcgact ggaacaacgc cacgacaccg gtctacgacc aagacggaga cgccacgacc
    15961 ttcagcaacg gcgagctggc cagcatcgtc gagatctgga cgcgggtcgc ggaggacttc
    16021 gctccgttca atatcgacgt gaccacggtc gatccgggcg gccaggccaa ccaggtggtg
    16081 gcccgcatcg caatcggcgg caactggtcg gactggtacg gcagtacttc cggcggcgtg
    16141 gcgtatgtcg gcggcttcta caaccccgcg ccgaacgtgg gctacgtgtt cgagaacgcg
    16201 ctgggcaacg ggaatgcaaa attcgtcgcc gaagctgcca cgcacgaagc cgggcacctg
    16261 ttcggcctgc agcaccaggc gctgtggagc gg
//
LOCUS       DASRJB010000359         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4049743, whole genome shotgun sequence.
ACCESSION   DASRJB010000359 DASRJB010000000
VERSION     DASRJB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4049743"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFB80_09505"
     CDS             complement(<1..961)
                     /locus_tag="VFB80_09505"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HZN34044.1"
                     /translation="MVRFAACFFVACSSLALAADVPPVIRSAKSGPWSSAETWEGGKV
                     PAAGARVHVLAAHTITYDVKSDDVIRALNVSGTLTFAKDKDTLLNVGLIKVEPNDVFS
                     EEGFDCDHVSETLDPSKPRATLEVGSPLAPIAAGKKAVIRLHYIEGHNALSCPAIVTC
                     GGRMDFHGTPLACTWTKLSATAKKGATEVACSDAVTGWRVGDQIIVTAGEADQHTEGL
                     TEERTLTAIDGQTLKFSEPLAHEHSGEGDFRCEVANLSRNVVVESAAPDGVRGHTMYH
                     RGSAGSISYAEFRHLGKKDTLGRYSLHFHLCGSTMRGSYVIGAS"
     gene            complement(1068..1598)
                     /locus_tag="VFB80_09510"
     CDS             complement(1068..1598)
                     /locus_tag="VFB80_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZN34045.1"
                     /translation="MDSPTAADTSRRPAPVARILLASCLGLGYSPIMPGTCGALLGPL
                     FYIPLALAVPQEPWQTLLIGGLLAAATWITIGLGRWSESYYGQKDCQKFVTDEVVGFL
                     FTVLLFHLPAQPVVTALWAFPITRVIDILKIPPARRLEKLPAGWGVVADDLLGSLYAA
                     AILHALFFAFPAWFGR"
     gene            1734..2054
                     /locus_tag="VFB80_09515"
     CDS             1734..2054
                     /locus_tag="VFB80_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34046.1"
                     /translation="MPNDQVITYIVDKLREGESIDRITKAIKGMRPDQARELVLKVRG
                     IMKWEVRKQAFWKMLGSFLLLLVFGGIFLATGRLFYIILPFAAAAFLWGAFQLVFALG
                     YDAD"
     gene            2210..3637
                     /gene="tssK"
                     /locus_tag="VFB80_09520"
     CDS             2210..3637
                     /gene="tssK"
                     /locus_tag="VFB80_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HZN34047.1"
                     /translation="MQNAPVNWYEGLFLRPHHFQAADRYWAETLRASEQWDHPCSYGL
                     LAIDFSREALGNQQFEVRLLKARMRDGTLVALEHGQEPDRVGLSEAFDKKEVLTVYLG
                     VPKLKLGRPNVGNHDSASPARFVPVRLGLPDEQTGSNEQELELKQLNARLLLSTQDLS
                     GYELLPIAQVKRASAGAARPQLDDSYIPPVIAIDAWQGLGRDIVRAIFDVIGQKIEVL
                     SQQIASRNIGLDSRNPGDLDRILMLGQLNAASSTLSIMGFAPGTHPLTAYTELCRICG
                     QLSIFAKDRRAEPVPPYDHDNLAGIFKEIYLRIERLLNAVRDYEYEQRAFTGVGLGMQ
                     VTLEPKWFNSDWQWYIGVNKGDLTTQECQELLSSGQLDWKLGSSRQVEILFKQRAPGV
                     EMRPLDRPIRALPSLSDWLYYEIHKSEGPAWRDVQATQTLAMRLRDSLIVNLDQLQGK
                     QQMVISNRGRNYPLQFALFAVPTRA"
     gene            3676..4305
                     /locus_tag="VFB80_09525"
     CDS             3676..4305
                     /locus_tag="VFB80_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HZN34048.1"
                     /translation="MTPKFAQAIDPVMLHVLGLLDRISRDERPSPADERLRIRGLVDQ
                     AEALLGASQQWKLGKYALAAWTDEMLVDAPWDGRDWWSNNVMEMELFSTRECYDRFYI
                     LAKEASTLPTRDALEVFYICVVLGFRGLYRDPEFSASTIQQLGLPPNVEAWARQASMA
                     IRLGQGRPNLTGKKRELSGAPPLRGRHIALWTSLAFVMAATVLAVMLLY"
     gene            4413..4862
                     /locus_tag="VFB80_09530"
     CDS             4413..4862
                     /locus_tag="VFB80_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34049.1"
                     /translation="MAELPATTAVVSAAQPLERPAVKVEEGPLAITWEQLDLPIPPDS
                     VFEPWMITTRVQALEGKQVRIKGYMFGGLFQTSGIREFPLLREKDCPFGQGGQPHHAI
                     NVELAGSLRTSLTTEPVTVEGKFSVQPWTGPNGKTWSVYHMEVTKME"
BASE COUNT          914 a         1611 c         1710 g          887 t
ORIGIN      
        1 tcgaggcccc gatcacgtag ctgccccgca tcgtactgcc gcagaggtgg aagtgcaggc
       61 tgtagcggcc cagcgtgtcc tttttgccca ggtggcgaaa ctcggcgtag ctgatcgacc
      121 cggctgagcc gcggtgatac atggtgtgcc cgcgaacgcc gtcgggggcg gcagactcca
      181 cgacgacatt gcggctcaga ttcgccactt cgcagcgaaa atcgccttcg ccggaatgct
      241 cgtgggccag cggctcgctg aacttgagcg tctggccgtc gatcgccgtc agtgtccgct
      301 cttcagtaag gccttcggtg tgctgatcgg cctcgccggc ggtaacgata atctggtcgc
      361 cgacgcgcca accggtcacg gcgtccgagc aggcgacttc cgtcgcgcct ttcttggcgg
      421 tggcgctcag cttggtccag gtgcaagcca gcggcgtgcc gtgaaagtcc atccgcccgc
      481 cgcaggtgac gatggccggg caagacaggg cgttatggcc ttcgatgtag tgcagccgaa
      541 tgacggcctt cttgcccgcc gcgatcgggg ccagcggcga gccgacctcg agcgtcgccc
      601 gcggtttgct cgggtcgagg gtctccgaga catggtcgca gtcaaaacct tcttcgctga
      661 acacgtcgtt gggctcaact ttgatcagcc cgacgttgag cagcgtgtct ttgtccttgg
      721 cgaacgtgag cgtgccggag acattgagcg cccggatcac gtcatcggac ttgacgtcgt
      781 acgtgatcgt gtgggctgcc agcacgtgga cccgcgcgcc ggctgcggga accttgccgc
      841 cttcccacgt ttcggcgctc gaccaggggc ccgatttggc gctgcgaatg accggcggga
      901 catccgcggc caacgcgagc gaagagcagg cgacgaaaaa gcaagcggcg aaacgtacca
      961 tcggcggggg ctccaaaggc gggaggtaaa caaacgggag cggctgccag aatacaccgc
     1021 agttggtcgg gctggcaact gtttgggctg ccttgaggga gcggcggcta acgtccgaac
     1081 cacgccggga aagcaaaaaa cagcgcgtgc aggatcgccg cggcgtagag cgagccgagc
     1141 aagtcgtcgg cgacgacgcc ccagccggcg ggcagctttt cgaggcgccg ggccggggga
     1201 atcttgagga tgtcgatgac ccgcgtgatc ggaaaggccc acagcgccgt cacgacgggc
     1261 tgtgccggca gatgaaacag gagcacggtg aagaggaagc ccacgacctc gtctgtgacg
     1321 aacttctggc agtccttctg gccatagtac gactcggacc agcggcccag cccgatcgta
     1381 atccacgtcg cggccgccag cagtccgccg atcagcagcg tttgccaggg ctcctgcggc
     1441 accgccagcg cgagcggaat atagaacagc ggccccagca gcgccccgca cgtgcccggc
     1501 atgatcggcg aatagccgag ccccaagcag ctggccagca ggatgcgtgc gacgggcgct
     1561 gggcggcggg aagtgtcggc ggcagtcggc gaatccatgc ggccagcgtg ccatttgcgg
     1621 cccgtcgcgg caaggtggca cgggagcttc cgccggcggc cgagtttttg cgttggccca
     1681 gccccacgtt agactgtcaa cgcgccactc cttcttttgc agactcgacg cctatgccca
     1741 acgatcaggt catcacctat atcgtggaca agctccgcga gggggagagt atcgatcgga
     1801 tcacgaaggc catcaaaggc atgcggccgg accaggcccg cgagctggtg ctgaaagtgc
     1861 gcggcatcat gaaatgggaa gtgcgcaagc aggcgttttg gaaaatgctt ggcagtttcc
     1921 tgctgctgct cgtgtttggc ggaatctttc tggcaacggg acgcttgttc tacatcattc
     1981 tgccgtttgc cgccgcggcg tttttgtggg gcgcctttca gcttgtgttt gcgctgggct
     2041 acgacgccga ctagggacgg actgcgccac gcggagccgt gtattcccgt ttttctaagt
     2101 cgcctaatct gctaggttgt gaatctcgcg aagctagccc tcgtaactgg cacttgactc
     2161 tccgctcccg cgctgcgccg gcggaggccc ggcagcgccg ttcaacgaca tgcaaaacgc
     2221 acccgtcaac tggtacgaag ggctgttcct tcgcccgcac cattttcagg ccgccgaccg
     2281 ctattgggcc gagaccctgc gggcctcgga gcagtgggac cacccgtgca gctacggctt
     2341 gctcgccatc gacttcagcc gtgaggccct cggcaatcag cagttcgagg ttcgcctgct
     2401 caaggcccgg atgcgggacg gcacgctggt ggccttggaa cacgggcagg agcccgaccg
     2461 cgtcggcctg agcgaggcct tcgacaaaaa ggaagtcctg acggtctatc tgggcgtgcc
     2521 gaagctcaag ctcggccggc ccaacgtcgg caatcacgac tctgcatcgc cggcccgttt
     2581 tgtgcccgtc cgcctcggcc tgcccgacga acagaccggc agcaacgagc aggagctgga
     2641 gctgaagcag ctcaacgccc ggctgctgct ttcgacgcag gacctgtcgg gctatgagct
     2701 gctgccgatc gctcaggtga agcgggccag cgccggcgcg gcgaggccgc agcttgacga
     2761 ctcgtatatc ccgccggtca tcgccatcga cgcctggcaa ggtttggggc gcgacatcgt
     2821 gcgcgcgatc ttcgacgtca tcggtcagaa gatcgaagtc ctcagccagc aaatcgccag
     2881 ccgcaacatc ggcctcgaca gccgcaatcc gggcgacctc gaccggatcc tgatgctggg
     2941 gcagctcaac gcggcttcct cgacgctgtc gatcatgggc ttcgcccccg gcacgcaccc
     3001 gctcacggcg tacacggagc tgtgccgcat ctgcgggcag ctgtcgattt tcgcaaagga
     3061 ccgccgggcc gagcccgttc cgccgtacga ccacgacaat cttgccggca tcttcaagga
     3121 gatctacttg cggatcgagc ggctgctgaa cgccgtgcgc gattacgaat acgagcagcg
     3181 ggcgttcacg ggcgtcggcc tggggatgca ggtcacactg gagcccaagt ggttcaactc
     3241 cgactggcag tggtacatcg gcgtcaataa gggggacctc accacgcagg agtgccagga
     3301 gctgctctcg tcgggccagc tcgactggaa gctcggcagc agccggcagg tggagatcct
     3361 gttcaagcag cgagcgccgg gcgtcgagat gcgtccgctc gaccggccga tccgggcgct
     3421 gccgtcgctg tcggactggc tgtattacga gatccacaag agcgagggcc cggcctggcg
     3481 cgacgtgcag gccacgcaga cgctggccat gcgattgcgg gactcgctga tcgtgaacct
     3541 cgaccagctg cagggcaagc agcagatggt gatcagcaac cgcgggcgca actatccgct
     3601 gcagttcgcg ctgttcgcag tgccgacgag agcgtgatgc cgtcccctct ccgataggga
     3661 gagggagagg aaacgatgac tcccaaattc gcccaggcca tcgacccggt gatgctccac
     3721 gtgctggggc tactcgaccg catttcgcgt gacgagcggc cgagcccggc cgacgagcgg
     3781 ctgaggattc gcgggctggt cgatcaagcc gaggccctgc tgggggcgag ccagcagtgg
     3841 aagctcggca agtacgccct ggcggcgtgg accgacgaaa tgctggtgga tgctccctgg
     3901 gacggccgcg actggtggag caacaacgtc atggagatgg agctgttcag cacgcgcgaa
     3961 tgctacgacc ggttctacat cctggcgaag gaggcttcga cgctgccgac gcgcgacgcg
     4021 ctggaggtct tctacatctg cgtcgtcctc ggctttcgcg gcctttatcg cgaccccgag
     4081 ttctccgcct caacgatcca gcagctcggc ctgcccccga acgtcgaagc ttgggcccgt
     4141 caggcctcga tggcgattcg cctgggccag ggccgcccca acctcaccgg caagaagcgc
     4201 gagctctctg gagcgcctcc tctccgcggc cggcacatcg ccctttggac cagcctcgcc
     4261 ttcgtcatgg ccgcaacggt gctggcggtg atgctgctgt attagtgcag cccgctcatt
     4321 aaaatggtga tgtgattgcg aaactcggca atttgttctg ctggctgcca gcaatggggc
     4381 tgttgccgat cattgctggt tgcgatcgga acgtggccga attgcctgct acgacggcgg
     4441 tagtctcggc cgcgcagccg cttgagcggc cggcggttaa ggtggaggaa gggccgctgg
     4501 cgatcacctg ggagcagctc gacttgccga ttccgccgga ctcggtgttt gagccgtgga
     4561 tgattacgac gcgggtccag gcgctggaag gcaagcaggt gcggatcaag ggatacatgt
     4621 tcggcggctt gtttcaaacg agcggcattc gcgagtttcc gctcctgcgg gagaaggact
     4681 gtcccttcgg ccagggcggg cagccgcacc atgccatcaa tgtcgagcta gcgggaagtt
     4741 tgcggacgag cctgacgacg gagccggtga ccgtggaagg gaaattcagc gtacaaccct
     4801 ggacgggccc caacggcaaa acgtggtcgg tttaccatat ggaagtgacg aagatggaat
     4861 agggccagga gctgcgctag agagaggtga tcgatgaaga cgcgtgttgc attcgctatc
     4921 ggactcgcct gcctgttcgc tgcggggtgc ggagtgcagt cgtcggcgag tcatgcaccc
     4981 acggatgttg ccgtggccac tgccgcgcag gttggcgatg ctcgcacgcc cacggaccag
     5041 tccccaactg tcgaaccagc tgagccggcc gacgcgcccg cggaggctcc agcacctgcg
     5101 gccgccgccc caccagccag tg
//
LOCUS       DASRJB010000360         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3482799, whole genome shotgun sequence.
ACCESSION   DASRJB010000360 DASRJB010000000
VERSION     DASRJB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482799"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..726)
                     /locus_tag="VFB80_09535"
     CDS             complement(70..726)
                     /locus_tag="VFB80_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34050.1"
                     /translation="MSSQTGRRRWQFSLLTLLIAMSGVALMCLALRSPNEAWATAVFV
                     AAAGSLALAALAAIYRDGRTRAFAIGFLVVGAAYWGLVLLAESNPEYGQPRLPTTRWT
                     IALFTLLHQDSMNSTTVMTAVYSAPVSYQGPAYPPAPPPPIPAGSTAGSPVYPSTGPP
                     VVSYVPQVIQRPLYAMQTFIDISHQSLVLLLGLVGGIVAQWLYATRREEPPPTAAPAG
                     "
     gene            824..2017
                     /locus_tag="VFB80_09540"
     CDS             824..2017
                     /locus_tag="VFB80_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34051.1"
                     /translation="MTTNGSLNRKLRMALVGGGQGAFIGRVHATAAVLDNRAVLVAGC
                     LSSTPERAKASAPDYDIPAERAYTSIGELVQKEKALPADKRIDFVSVATPNHTHFEIA
                     KAAAEAGFNVMCDKPMTFDLAQAEALAEVVDRTGVVFAVTHNYTGHPLVRQARAMIAA
                     GELGEINAVRAFYIQGWLRTRLENSDQKQASWRTDPKRSGAAGCFGDIATHAFNLGRY
                     MTGLLPAEISCHLKSFEPGRQLDDYGTAVIRYKNGALGTVTASQITHGRENDVYIEID
                     GTKGSLEWHQENPNQLFFRKNGEPQRIYTRAGGPYLSPAAAAATRIPSGHPEGYLEAF
                     ANIYRSAFDDMVLRAEGKPFEKVNTIYPNIADGVEGMLFIQQCVASSKQNGAWLPLAH
                     KRSRS"
     gene            complement(2068..2790)
                     /locus_tag="VFB80_09545"
     CDS             complement(2068..2790)
                     /locus_tag="VFB80_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZN34052.1"
                     /translation="MTRLPFTALCLLLIADRSEAAIYTLGVSNFASPSPFGQIEVIRT
                     GSNTATVNLTAFTSGSTGYLFGNGGIIGLNLNATGTSSISSISYTQPALINGNTAGAL
                     ALDPIGTGNLNGFGDFNFKLTNFDGATHAVRTLSFTLANTIANWTTDADVLTANDKGY
                     VAGGHVFTYNIAADDNYIDVNLTGYAVNGAGGEGGGGGEGGGGGGGEQMPEPTSLAVL
                     SLLIAGAGAGSWLSRRKRAKHA"
     gene            3206..3550
                     /locus_tag="VFB80_09550"
     CDS             3206..3550
                     /locus_tag="VFB80_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34053.1"
                     /translation="MVQKNTVRVVTRASDGTLRIRDYPTTEPLLQMHTQIGIDDCSTD
                     LGLRGLPVFRGLIGPMPEGKNIVRYESPDVFETLTKEWSSAKNPRRRRRMQPPPGLGE
                     VQPALPSLGPAP"
     gene            complement(3686..>4222)
                     /locus_tag="VFB80_09555"
     CDS             complement(3686..>4222)
                     /locus_tag="VFB80_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34054.1"
                     /translation="EVHGSTLGIIGLGRIGRQIARRARGFEMTVLYHNRRRDEAAEVS
                     LGVRYAALGELLAASDYVVLSCPLTDATRGLIGAAALAKMRPTATLVNISRGPVVDTA
                     ALTAALQERRIYAAALDVTDPEPLPRDHPLLALDNVIIAPHLGSATEQTRQRMAEISV
                     ENLFRGLASQPLLFEVRG"
BASE COUNT          788 a         1343 c         1358 g          733 t
ORIGIN      
        1 cgttgcggcg ggtgcagttg cgagcacaga atgccgctcg gaaaaacttg gcggaatgcg
       61 cgcatcgccc tagccggccg gcgccgccgt gggcggcggt tcttcgcggc gggtggcgta
      121 gagccactgg gccacaatgc cgccgaccag gccgagcagc agcacgagcg actggtggct
      181 gatgtcgata aacgtctgca tcgcgtaaag cggccgctgg atcacctggg ggacgtacga
      241 cacgaccggc gggcccgtcg agggatacac aggcgaaccg gccgtcgagc cggccggtat
      301 gggaggtggg ggcgcgggcg ggtaggccgg cccctgatag ctgaccggcg cggaatagac
      361 cgccgtcatg accgtcgtgc tattcatgct gtcctggtgc aacagcgtga atagcgcgat
      421 ggtccaccgc gtcgtcggca atcggggctg gccatactcc gggttgctct cggcgagcag
      481 gaccaggccc cagtaggccg cgccgacgac gagaaagccg atggcaaagg cccgtgtccg
      541 gccgtcgcga tagatggccg ccagcgccgc cagcgccaga gagccagcag ccgccacgaa
      601 cacggccgtc gcccaggcct cgttcggcga gcggagcgcc aggcacatca gggccacgcc
      661 gctcatggcg atgagcagcg tgaggagcga gaactgccac cgccgccgac ccgtttgact
      721 cgacatgcaa cacctccttg cctgctaact ggcatctcac atttgttttg atacaacaaa
      781 gagccgcatt tggccaatat tcgctgttca ggaatcctct cccatgacga ccaacggctc
      841 actcaaccgc aagctccgca tggccctcgt cggcggcggc cagggggcgt ttatcggccg
      901 cgtccacgcc accgccgcgg tgctcgacaa ccgggcggtc ctcgtcgcgg gctgcctgtc
      961 gagcacgccg gaaagggcca aggcgtcggc ccccgattac gacatcccgg ccgagcgggc
     1021 ctatacgtcg atcggcgagc tggtccagaa agagaaggcg ctgccggcgg acaagcggat
     1081 cgatttcgtc agcgttgcca cgccgaacca cacgcacttc gagatcgcca aggccgctgc
     1141 cgaggcgggc ttcaacgtga tgtgcgacaa gcccatgaca ttcgacctcg cccaggccga
     1201 ggcgctggcc gaggtggtgg atcgcacggg tgtcgtcttt gccgtgacgc acaactatac
     1261 cgggcatccg ctggtgcggc aggcccgcgc gatgatcgcc gccggcgaac tgggcgagat
     1321 caacgccgtc cgcgctttct atatccaggg ctggctccgc acgcggctgg agaattctga
     1381 ccagaagcaa gcctcgtggc ggaccgatcc caagcggagc ggcgctgccg gctgcttcgg
     1441 cgacatcgcc acgcacgcct tcaacctggg ccggtacatg acaggcctgc tgcccgccga
     1501 gatcagctgc cacctcaagt cctttgagcc cggccggcaa ctcgacgact atggcaccgc
     1561 ggtgattcgt tacaaaaacg gcgccctcgg cacagtgacc gcttcgcaga tcacgcacgg
     1621 ccgcgagaac gacgtctata tcgagatcga cggcacgaag ggctcgctgg agtggcacca
     1681 ggagaatccg aatcaactat tcttccgcaa aaacggcgag ccgcagcgga tctacacgcg
     1741 ggccggcggc ccctatttga gtcccgcggc cgccgctgcc acgcgaattc cgagcggcca
     1801 tcccgagggc tatctcgaag cctttgccaa tatctaccgc tcggcgttcg acgacatggt
     1861 cctgcgtgcc gaaggcaagc cgttcgagaa ggtaaacacg atttacccga acatcgccga
     1921 cggcgtcgag ggcatgcttt tcatccagca gtgcgtcgcc agcagcaagc agaacggcgc
     1981 gtggctgccg ctggcgcaca agcgatcgcg cagttgagcg cagcttagaa cagttgcgag
     2041 ccgacgcagc acttcggctt gaagaagtta agcgtgcttt gcccgcttgc gccgcgacag
     2101 ccacgatccg gcgccggcgc cagcgattaa gagcgaaaga actgccaggg aggtcggttc
     2161 gggcatctgt tcgccgccgc ccccgccacc gccttcgcca ccaccacccc cctcgccacc
     2221 cgcgccattc acggcgtatc cggtgagatt tacgtcgatg tagttatcgt ccgccgcgat
     2281 gttgtaggta aacacgtgcc cgcctgccac atagcctttg tcgttagcgg tcagcacgtc
     2341 ggcatccgtg gtccagttgg cgatcgtgtt cgccagcgta aacgacaggg tccttacggc
     2401 gtgagttgcc ccgtcgaagt tagtgagctt gaaattgaaa tcgccgaagc cattcaaatt
     2461 gccggtaccg atgggatcga gtgcgagtgc gccggcggtg ttgccattga tcaacgctgg
     2521 ctgcgtatag ctgatcgagg agatcgacga ggtgccagtg gcgttcagat tcaggccaat
     2581 gatgccaccg tttccaaaca ggtagccggt gctgccggat gtaaacgcag tcaggttgac
     2641 cgtggcagtg ttgctgccgg tgcggattac ctcgatttgt ccaaaaggtg atggcgacgc
     2701 gaaattggag acgccaagcg tgtagatggc ggcttcgctc cggtcggcga tcaggagcag
     2761 gcatagggcg gtaaagggca gtcgggtcat ccacgttctc cgattcgccc caagcccttg
     2821 aaagcttcac aactccagga cgtgcattgg tcctgttact tcttttgaac cgcgctgggg
     2881 ggttgcctga cgcgctggcc gcattttttt acttttttcg ctagattttt tccttgcgat
     2941 tgagctggcc gcaaattgtt tctgcataag gatttggaga gaactcttgg caggtcatcc
     3001 ataacggtct cgggcggcag ggaaggtttt tcagccttta aggcatgccg gacacgatct
     3061 gtgccgccgc ccaccaacgt gccgatacct gctgagcaag acagccggcg ggagcttcgt
     3121 gcgactacga agtcggcggc tagtggttac tggctcggcg tggcatcaga cggacctgat
     3181 cgcgcccatt taatggggtt cgaccatggt tcagaagaac acggtgaggg tggtaacgcg
     3241 cgctagcgac ggcacgctcc gcattcggga ctatccgacg acggagccgc tgctgcaaat
     3301 gcacacgcag atcggaattg acgactgttc gaccgatctg gggcttcgcg gattgcccgt
     3361 ctttcgcggc ctgatcggcc cgatgcccga aggcaagaac attgtccgct acgaatcgcc
     3421 cgacgtattc gagacgctca ccaaagagtg gtccagcgcc aagaaccccc gccgccgccg
     3481 gcggatgcag ccgccgccag gcctgggcga agttcagccg gcgctgccct cactggggcc
     3541 ggcaccatag ttcctgatcc cggttcccaa gctccctggt tccgaagctc cgctttggaa
     3601 cccacgtctg gaagctccgc ttctcgatcg caaagcagag cctcggtgct ggcgttccca
     3661 agcagagctt ggtaacgaag tagaactagc cgcggacctc aaacagcagc ggctggctgg
     3721 cgaggccgcg gaagaggttt tcgaccgaga tctcggccat ccgttgccgc gtctgctcgg
     3781 tggcactgcc caggtgcggc gcgatgatca cgttatcgag cgccagcagc ggatggtcgc
     3841 gcggcagcgg ctcgggatcg gtcacgtcga gcgcggccgc atagatgcgg cgctcctgca
     3901 aagccgcagt cagcgcagcg gtatcgacga ctggcccgcg cgagatgttg acgagcgtag
     3961 ccgtcggccg catcttggcc agcgcagccg cgccgatgag cccgcgcgtg gcgtcggtga
     4021 gcgggcagga gagcacgacg tagtcggagg ctgccagcag ctcgcccagg gctgcgtagc
     4081 gcactcccag cgatacttcg gccgcttcgt cgcggcggcg gttgtggtag agcacggtca
     4141 tctcgaagcc gcgagcccgc cgggcgatct gccggccgat ccgccccagg ccgatgatcc
     4201 ccagcgtgct gccgtgcact tc
//
LOCUS       DASRJB010000361         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_952155, whole genome shotgun sequence.
ACCESSION   DASRJB010000361 DASRJB010000000
VERSION     DASRJB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952155"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..>2752)
                     /locus_tag="VFB80_09560"
     CDS             complement(200..>2752)
                     /locus_tag="VFB80_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN34055.1"
                     /translation="CHGEDGASEGGFNFVLNLEKLARTVVKPKNLAGSLLWERISATD
                     ESVMPPEGEKPRPTPEEIALVKAWIEAGAPALPTAKPREFIPNEQVVKWVYADVQKAA
                     ERSRRFQRYFTLTHLYNAGVSEDELQTYRNAFFKLINSLSWNTDLVLPEALDPAKTVF
                     RIDIRQLNWSGEQWQQVESANPYFLALNSAEALACNTATQSNMPYVRIDWFVFAASKP
                     PLYHAMLALPETAAELEAVLHVNAEANIEQEKAIRAGFNRSGVSQHNRLIEWHKSPYG
                     SYWKSYDFGGSTGRQNLFEYPLGSGGSSADSFQHDGGELIFSLPNGMQGYMLVDGSGR
                     RIEQGPTNIVSDPKQPDRLVTNGVSCMSCHYTGVIPKTDEVGKAVRANPKAFENAEDI
                     LALYREPEHLNRVLDEDARRFAAAMKKLGMTSLSRSGEAVSAMALRFQQELDLPLAAC
                     EFGVTPDDLLKRLDGTAVSARRFAPLRVPGGTIKRDVFVAGFGEAALELRLTSESRSG
                     AGIPNNGPTPAIASTAPRPAVASPPASSAAMARLPNTADGKPGELRRFTDLRWGAKSL
                     AFSFDGSLLAAGKPDREVKLFDVVKNAEIGSNDKLDLLASVEYCAFTPFGLNLLLAGR
                     SGQIQIFQVSRNGQFKETGQFVGHSQQVNCLSLSADGKFAVSGSQEKKLRYWEVSSGR
                     EHGVFAGFEGGVKACHLARNGRTALATDGQTLLTIDCAKRTVTASRKLTNSWASGQAA
                     AFSADGELVAIGDSYAIRVWNLTTGAELPVLQDNEIQWSMVFTPDGTRLISGGSGKVN
                     VWDPRKGRKLSVISGGQGYIKALAASPDNKHAAAAVNSDSDVIVVRLPPPEK"
BASE COUNT          465 a          871 c          900 g          516 t
ORIGIN      
        1 tagtctattg ctgggtttgc gaagtagcac attccgaaga aaaaccgcgg atcacgcgag
       61 ccgttgctct actgcgcagc agttagcgga gcttccagac ttgcgtgccc aagcagagct
      121 tgggcacgat atgaaacgag atgaagtgct ttgagaacga tcgctccctt ggcgggaaac
      181 gagagccgcc gactcggcgt tacttttccg gcggcggcaa ccgcaccacg atcacgtcgc
      241 tgtcgctgtt gacggcggcg gctgcgtgct tgttatcggg cgaggcggcc agcgccttga
      301 tatagccctg gccccccgaa atcacgctga gcttgcgtcc tttgcgtgga tcccagacgt
      361 tgaccttgcc cgagccgccg ctgatcagcc gcgtgccgtc gggcgtgaag accatgctcc
      421 actgaatttc gttgtcctgg agcacgggca actccgcgcc ggtggtaaga ttccagacgc
      481 ggatggcata ggagtcgccg atggccacca attcgccgtc ggccgaaaag gccgccgcct
      541 ggccggacgc ccacgagttg gtcagctttc gcgaggcggt gacggttcgc ttggcgcagt
      601 cgatcgtgag cagcgtctgc ccgtcggtgg ccagcgcggt gcggccattt cgcgcgaggt
      661 ggcaagcctt gacgccgcct tcaaatccgg caaagactcc gtgctcccga ccgctggaga
      721 cttcccaata ccgcagcttc ttctcctggc tgccagagac cgcaaacttg ccgtccgcgg
      781 aaagggagag gcagttcacc tgctgggagt gcccgacgaa ctgtccggtc tccttgaatt
      841 gcccgttgcg gctgacctgg aaaatctgaa tctggcccga ccggcccgcc aggagcaagt
      901 tgaggccgaa cggcgtgaac gcgcaatact cgacagaggc cagcagatcc agtttgtcgt
      961 tcgaaccgat ttcagcgttc ttgacgacgt cgaacagctt gacctcccgg tcgggcttgc
     1021 ccgcggccaa gagtgaaccg tcgaaggaaa atgccagcga tttggcgccc cagcgcaagt
     1081 cggtaaaacg ccgcagctcg ccgggcttgc cgtcggccgt gttgggcagg cgagccatcg
     1141 ccgcgctgct cgcgggagga ctggcaaccg ctggccgcgg cgctgttgaa gcgatagccg
     1201 gcgtggggcc gttgtttgga attccagcgc ccgaccgcga ctcgctggtc aggcgcagct
     1261 ccagcgccgc ctcgccgaag ccggcgacga acacgtcgcg cttgatggtg ccgccgggca
     1321 cgcgcagcgg agcaaaccgg cgggcgctga ccgccgttcc atccagccgc ttgagcagat
     1381 cgtcgggcgt gacgccgaat tcgcaggccg ccagcggcaa atcgagctcc tgctggaatc
     1441 gcagggccat cgctgaaacc gcctcgccgc tgcggctgag gctggtcatg cccagcttct
     1501 tcattgcggc ggcgaaccgg cgggcgtcct cgtcgagcac gcggttgaga tgctcgggct
     1561 cgcggtagag ggccagaatg tcctcggcat tctcgaacgc cttagggttg gcccgcacgg
     1621 ccttgccgac ctcgtcggtc ttgggaatca cgcccgtgta gtggcacgac atgcatgaga
     1681 cgccgttggt caccaggcgg tcgggctgct tgggatcgct gacgatgttc gtgggcccct
     1741 gctcgatccg ccggccgctg ccgtcaacca gcatgtagcc ctgcatgccg ttggggagcg
     1801 agaagatcag ctcaccgccg tcgtgctgga acgagtcggc cgaggagccg ccggagccca
     1861 gcggatactc gaacaggttc tgacgcccgg tgctgccgcc gaaatcgtag ctcttccagt
     1921 agctgccgta gggcgacttg tgccattcga tcaggcgatt gtgctgcgag acgcctgagc
     1981 gattgaaacc ggcgcggatc gccttttcct gctcgatgtt ggcctcggcg ttgacgtgta
     2041 gtaccgcttc caactcggcg gccgtttcgg gcagggccag catcgcgtga tacagcggcg
     2101 gcttgctggc agcgaacacg aaccagtcga tccgcacata gggcatgttc gactgcgtcg
     2161 ccgtattgca ggccagcgcc tcggccgagt tgagcgccag gaaataggga ttggccgact
     2221 cgacttgctg ccactgctcg ccgctccagt tcagctggcg gatgtcgatg cggaacacgg
     2281 tcttggccgg gtcgagggcc tcgggcagaa ccaggtcggt gttccacgac aggctgttga
     2341 tcagcttgaa aaaggcgttg cggtaggttt gcagctcgtc ctccgagacg ccggcgttat
     2401 agaggtgcgt cagcgtgaaa taccgctgga accgccgcga tcgctcggcc gccttctgca
     2461 cgtcggcata gacccacttg acgacctgct cgttgggaat gaattcgcgc ggtttggcgg
     2521 tcggcagagc cggcgcgccg gcctcgatcc acgctttgac cagtgcgatc tcctctggag
     2581 tcggccgcgg cttctcgccc tcgggcggca tcacgctttc gtccgtcgct gaaatccgct
     2641 cccacaacag cgagccggcc aggttcttcg gcttgacgac cgtgcgggcg agcttttcca
     2701 ggttgagaac aaagttgaag ccgccttcgc tggccccgtc ttcgccgtgg ca
//
LOCUS       DASRJB010000362         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2923302, whole genome shotgun sequence.
ACCESSION   DASRJB010000362 DASRJB010000000
VERSION     DASRJB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2923302"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFB80_09565"
     CDS             complement(<1..569)
                     /locus_tag="VFB80_09565"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZN34056.1"
                     /translation="MVQELVAGVHRFKREVFQGQQELFERLAGGQEPQTLFITCSDSR
                     IDPNLITHTDPGDLFVLRNAGNLIPAYGAASGGEVATIEFAVTGLGVRDIVVCGHSHC
                     GAMKGLLHPEYLAEMPAVAEFLRHAEATRRIVKSKYSHLHGEPLLDATIEENVLVQLE
                     NLQTHPAVAAALAGDQLKLHAWVYDIATG"
     gene            689..1666
                     /locus_tag="VFB80_09570"
     CDS             689..1666
                     /locus_tag="VFB80_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HZN34057.1"
                     /translation="MVSPWVAVLTSFHNTPIFGRIWVTVAAVTGYATLVVLLDRFVLP
                     HVETVRPEFHGFLGLILGTLLVFRTNTSYDRWWEGRKLWGQLVNDCRNLAIKVQTCVR
                     ADPEQKQRLGRWLCDFAWALKGHLRGGVHLTDLGGFKTDTSHPQHVPAYVSARIYDQF
                     EVWRQNDQLGGFELLFLDQHASSLMNICGACERIQKSPISISYRWFIRQSIAIYLLTL
                     PWGLVETFGWWDIPATAMLGYFMIGVEIIAEEIEDPFGTSADDLRLEDMCQTIERSVM
                     SILREEKLAPEMLPPPRPDVSQWEHVDSANMPGALPGDGDVSAPRPAGN"
     gene            1626..>2282
                     /locus_tag="VFB80_09575"
     CDS             1626..>2282
                     /locus_tag="VFB80_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34058.1"
                     /translation="MTATSPLLAQQAISGQQPLPTPGWVEMFDQGEADPGLKGFRTPR
                     GIKLEVAAQQPAVVNPRLLAFGDDGTPYVIEWRPGELREESQDVALEGGRSIKHFRTT
                     KSTRDVLKSLHDTNGDGVFDEARVVMDDLELPSGLLVNDGWFYWTSGGRVLRRRPFDP
                     MLAEQLAAAATEKRGPPTTVTGDGKWLEQLLISGLSGVIHFQAVGLSLGPDGCLYVAC
                     G"
BASE COUNT          389 a          752 c          721 g          420 t
ORIGIN      
        1 tcgccggtgg caatgtcata gacccaggcg tgcaatttca gctggtcccc cgcgagggcc
       61 gccgcaaccg caggatgcgt ctgaagattc tcgagctgca ccagcacgtt ttcttcgatt
      121 gtggcatcca agagcggctc gccgtgcaga tgcgagtatt tcgacttcac gattcgccgc
      181 gtggcctcgg catgccggag gaactcggcc acggccggca tctccgcgag atactcggga
      241 tgcagcaggc ctttcatcgc accgcagtgc gaatggccgc agacgacgat gtcccgtacg
      301 cccagccccg tcaccgcgaa ctcgatcgtt gccacctcgc cgccgctggc cgcgccgtac
      361 gccggaatca aattgcccgc gttccgcagc acgaacaggt cgcccgggtc ggtgtgcgtg
      421 atcaggttcg ggtcgatccg cgaatccgag caggtgatga acagcgtttg cggctcctgg
      481 ccgccggcca gccgctcgaa caactcctgc tggccctgaa agacctcgcg cttgaagcgg
      541 tgcacgccgg cgaccaattc ctggaccacg tctggcctcg tttcacaaag tagcgttcaa
      601 tagcaagccg acgcccaaaa cctaacgccg cctagcgccg tactcaaccc gccgccgtca
      661 ctcagcactc cgcattccgc attaggcaat ggtcagcccc tgggtcgccg tcctgacgtc
      721 gttccacaac acgccgatct tcgggcggat ttgggtgacg gtagccgcgg tgaccgggta
      781 tgcgacgctc gtcgtgctgc tcgaccggtt cgtgctgccg cacgttgaga cggtccgtcc
      841 tgaattccac ggcttcttgg gcttgatcct gggtacgctg ctcgtctttc gcaccaacac
      901 gtcgtacgac cgctggtggg agggccgcaa gctgtggggg cagctcgtca acgactgccg
      961 caacctggcg atcaaggtgc agacctgcgt gcgggccgac cccgaacaga agcagcgctt
     1021 gggccgctgg ctctgcgact ttgcctgggc cctcaagggg cacctccgcg gcggcgtcca
     1081 cctgaccgac ctgggcggct tcaagaccga cacctcgcac ccgcagcacg tgccggccta
     1141 tgtctccgcc cgcatctacg accagttcga agtctggcgg cagaacgatc agctcggcgg
     1201 cttcgagctg ctgtttctcg accagcacgc ctcgtcgctg atgaacatct gcggggcatg
     1261 cgagcggatt cagaagtcgc cgatctcgat ctcttaccgc tggttcatcc gccagtcgat
     1321 cgcgatctac ctgctgacgc tgccgtgggg cctggtcgag accttcgggt ggtgggacat
     1381 tcccgccacg gcgatgctcg gctacttcat gatcggcgtc gagatcatcg ccgaggagat
     1441 cgaagatccc ttcggcacct cggccgacga tctgcggctg gaggacatgt gccagacgat
     1501 cgagaggagc gtgatgtcga tcctccgcga agaaaagctg gcccccgaga tgcttccccc
     1561 gccgcggccg gatgtgtcac aatgggagca tgtggactct gcgaacatgc ctggcgctct
     1621 gcctggtgac ggcgacgtct ccgctcctcg cccagcaggc aattagcggc cagcagccac
     1681 tgcccacgcc aggctgggtc gaaatgttcg accagggcga ggccgatcca gggctcaagg
     1741 gttttcggac gccccgcggc atcaagctcg aggtcgcggc gcagcaacct gccgtagtca
     1801 atccgcgact gctggcgttt ggcgacgacg gtacgcccta tgtgatcgag tggcggccgg
     1861 gagagctgcg cgaggagtcg caggacgtcg ccctggaggg cggccgctcc atcaagcact
     1921 ttcggacgac caagtccacg cgcgacgtgc tcaagtcgct gcacgacacc aatggcgacg
     1981 gcgtgttcga cgaggcgcgc gtcgtcatgg acgatttgga gttgccttcc ggtctgctcg
     2041 tcaacgacgg ctggttctat tggaccagcg gcggacgcgt ccttcgtcgg cggccatttg
     2101 acccgatgct ggctgagcaa ctggcggcag ccgcgaccga gaagcgcgga ccgccaacga
     2161 cggtgaccgg cgatggcaag tggctggagc agttgctaat cagcggacta tcgggagtca
     2221 ttcattttca ggctgtcggc ctgtcactcg gcccggatgg ctgcctgtac gttgcttgcg
     2281 gt
//
LOCUS       DASRJB010000363         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_384660, whole genome shotgun sequence.
ACCESSION   DASRJB010000363 DASRJB010000000
VERSION     DASRJB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384660"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFB80_09580"
     CDS             <1..223
                     /locus_tag="VFB80_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34059.1"
                     /translation="RSEALRTLGRFASNPELSFSWYDAAVLSQRIRQTAIEQRAQAQH
                     PQPPAAEPSPLHTRPRFKLPLASDEQNKG"
     gene            294..1202
                     /gene="xerC"
                     /locus_tag="VFB80_09585"
     CDS             294..1202
                     /gene="xerC"
                     /locus_tag="VFB80_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZN34060.1"
                     /translation="MHAAITQFLRFLSVERNAAELTIKSYREDLTSLADYLAQACGRT
                     PSPGQVTPLDLRGYVSALHEAGYAKSSVARRLASLRTFYRFAQREGLAESNPAKPLRN
                     PRPDRKLPHFLSTSEIGTLLVAPPATTAQGIRDRAILETMYSAGLRVSEAVGLNDGDL
                     DLAEGLLRIRGKGRRERLAPVGSFAAKALLRWLKLRKLSPREPASQLAPVFTNKFGRR
                     LTTRSVQRLLLKHLKLAGLDLRTTPHTLRHSFATHLLDRGADIRSVQELLGHKSLVTT
                     QIYTHVSTAGLRAVYERAHPRARRSR"
     gene            complement(1209..1967)
                     /locus_tag="VFB80_09590"
     CDS             complement(1209..1967)
                     /locus_tag="VFB80_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34061.1"
                     /translation="MNAAPTLWSSDTVCLHADGVGVPAIARAMYERTCRTDFSAPGFC
                     LLNAGSERGSRGLRRLMVDLRQELATLHEARTGQTLNFVSVVRVDQQATTRPHLDGGP
                     EQSLLMLGYEPSAVRSELEISDYSRCAFDLGLTPKEFLGRHNPMFRSGSELLRAYTTR
                     IPCFSPADHQIAFINNSSTPYSADGATWQGVLHTATVPAPDENQRRVINSTMLASVPL
                     GTRESVSTAELDEFLKTTAVRRQGYDKPHLQDDA"
     gene            2032..2730
                     /locus_tag="VFB80_09595"
     CDS             2032..2730
                     /locus_tag="VFB80_09595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN34062.1"
                     /translation="MSRTDPQHQAARFDRVYRADDRTYGTEPSRELVRYVDQCVRGGR
                     GLDLAAGLGRDTLALVERGLVMTAVDVAEEGLKHLAEEARRRGLADRVTVVRQDIAAY
                     DYPLQALDVIVATTAFDHIERAKCLFILPKIASALTSAGVLYAEVHTTEDPGSPTGFG
                     HQLQAPVSETAGAVAHYFEPNELLHLLARHLRIVHYEERREWDQTHGRPHLHGKAIAL
                     AVPKTAHPPYRGDR"
BASE COUNT          546 a         1096 c          926 g          511 t
ORIGIN      
        1 gcggagcgag gccctgcgga cgctcggccg ctttgccagc aaccccgaat tgagcttcag
       61 ctggtacgac gccgccgtcc tcagccagcg aatccgccag accgccatcg agcagcgggc
      121 ccaggcgcag catccgcagc caccggccgc cgagccttcc ccgctgcaca cccggccgcg
      181 cttcaagctg ccgctggcga gcgacgagca aaacaagggc tagggcttgg ggctaggact
      241 agggttggat gcaccgcgcc tccagcccta gcccctagcc ctagcccatt ccgatgcatg
      301 ccgccatcac gcaattcctc cgctttctca gcgtcgagcg gaacgcggcc gagctgacga
      361 tcaagtcgta tcgcgaggac ctgacctcgc tggccgatta cctggcgcag gcctgtggcc
      421 gcacgccgtc gccgggccag gtcacgccgc tggacctgcg gggctacgtc tcggccctgc
      481 acgaagccgg ttatgccaaa tcgagcgtcg cccgccggct ggcgtcgctc cgcacgttct
      541 acagattcgc gcagcgggaa ggcctggccg agagcaatcc cgccaagccg ctccgcaatc
      601 cgcggcccga ccgcaagctg ccgcattttc tctcgacgag cgagatcggc acgctgctcg
      661 tcgcgccgcc ggcgacgacc gcgcagggga ttcgcgaccg ggcaatattg gaaacgatgt
      721 actcggccgg tctccgcgtg agcgaggcgg tcggcctgaa cgacggcgac ctcgatctgg
      781 ccgaggggct gcttcgcatc cgcggcaagg gccgccgcga gcggctcgcg cccgtcggca
      841 gctttgccgc caaggcgctg ttgcgctggc tcaagctccg caagctttcg ccgcgcgagc
      901 ccgccagcca actcgcgccc gtcttcacga acaagttcgg ccgccgcctg acgacccgca
      961 gcgtccagcg gctcctgctc aagcacctga agctggcggg cctggacctg cggacgacgc
     1021 cgcacacgct ccgccacagc tttgccacgc acctgctcga ccgcggcgcc gatatccgca
     1081 gcgtgcagga gctgctcggc cacaagagcc tcgtcaccac gcaaatctat acgcacgttt
     1141 cgaccgccgg cctccgcgcc gtctatgaac gggcccaccc gcgggcccgc cgctcccgct
     1201 agacctcctt acgcgtcgtc ctgcaagtgt ggcttgtcgt agccttgccg gcgaacggcc
     1261 gtcgtcttta agaactcgtc caattccgca gtggatacgc tttcccgtgt gcccagcgga
     1321 accgacgcca gcatcgtcga attaatcacg cgccgctggt tctcgtcggg agcggggacc
     1381 gtcgccgtgt gcagcactcc ctgccaggtc gctccgtccg cggaatatgg cgtcgagctg
     1441 ttgttgatga atgcaatctg atggtccgcc ggcgaaaagc acggaatacg cgtcgtgtac
     1501 gcccgcagca gctcggagcc cgagcgaaac atcggattgt gccgccccag aaactccttg
     1561 ggcgtcaatc ctagatcgaa ggcgcagcgg gaataatccg agatctccaa ttccgatctc
     1621 acagcgctcg gctcgtagcc cagcatcagc agcgactgct ccggaccgcc gtcgaggtgc
     1681 ggccgggtcg tggcctgctg atcgacgcga accaccgaaa cgaaattcag cgtctgaccc
     1741 gttctcgcct cgtgcagagt cgccagctct tgcctgagat ccaccatcag ccgccgcaag
     1801 cctcgcgatc ctcgctcact ccccgcattc agcaggcaga accccggcgc ggaaaagtcc
     1861 gtccggcacg tccgctcgta catcgcgcgg gcgatggccg gcactcccac tccgtcagcg
     1921 tggaggcaga cggtgtcgga cgaccacaat gtaggagcag cgttcattcg gggaattcta
     1981 ccagctcagc cgcggcgatt cggcccggca gttgcaagga tgggtcctgc tatgagccgg
     2041 accgatcctc aacaccaggc ggcgcgtttc gaccgcgtct atcgcgccga cgatcggacc
     2101 tatgggaccg agccgtcgcg cgagctcgtc cgctacgtgg atcagtgcgt gcgcggaggc
     2161 cggggcctgg acctggcggc ggggctgggc cgcgacacgc tggctctggt cgagcggggc
     2221 ctcgtcatga ccgccgtgga tgtcgccgag gaaggcctca agcacctggc cgaggaagcc
     2281 cgccggcgcg gcctggccga tcgcgtcacc gtcgttcggc aggacattgc ggcttacgac
     2341 tatccgctgc aggcgctgga cgtgattgtg gccacgacgg ccttcgatca catcgagcgc
     2401 gcgaaatgcc tcttcattct gccgaagatc gcttcggccc tgacgagtgc cggtgttttg
     2461 tatgccgagg tgcataccac ggaggacccc ggctcgccca ccggcttcgg tcaccagctt
     2521 caagcgccgg tcagcgagac agccggggcg gtcgcccatt acttcgagcc caacgagctg
     2581 ctgcacctcc tcgcccggca cctgcggatc gtccactacg aggagcgtcg cgagtgggac
     2641 cagacccacg gccgcccgca tctccacggc aaagccatcg ccctcgccgt tcccaaaacc
     2701 gcccacccgc cctaccgcgg cgaccgctaa ggggcgcatt gccgttttgg ttacggaacc
     2761 atttacgccg ctaagccctt ccgcggcagg ctggctcgag ccggtttgtg accaggcgac
     2821 aaacgggccg caatcgctcc aacttcgcac cgaacataca cttacgatca ctatataact
     2881 ttacgggtac attagcaaca gaagcgatag aagacttaga gggcgacaga taacggttag
     2941 tgcaacggcg tcttcatttc ccttggaatg cgaacggtca cgagctcctc ttcgaatccg
     3001 gagaccgcaa ccgatccacc cctgatatga ttcgatgcgc acatccgaac atttattccc
     3061 tactgcgcca aatactttt
//
LOCUS       DASRJB010000364         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_101967, whole genome shotgun sequence.
ACCESSION   DASRJB010000364 DASRJB010000000
VERSION     DASRJB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101967"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1430)
                     /locus_tag="VFB80_09600"
     CDS             complement(177..1430)
                     /locus_tag="VFB80_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN34063.1"
                     /translation="MKTSRRALLAAAAAGSCALVARARAADALRVPRAELDRILNQPV
                     LTTDFVKEPVIVESVELLVNGRNDLVRTRSAAGVEVVTVPNPERMAQTYPIFLKNIVP
                     VFLKQDARKLESLLWDVYRHSSNYKLQGIALWPGVAAIEMALLELMGQTAGRPLADFF
                     GGRSRDEIAVYYASGNRGNTPEAEVEYLQKLVAGSGAKAIKFRLGGRMNKNVDSLPGR
                     TEALIPLVRKTFGDALTLYADSNSSYDAKEAIRIGRLMEQHGYGFYEEPCPFDDLWDT
                     KEVADALTIPVAGGEQEFSMARWRWAVAERGLDIVQPDLHYGGGFIRATQVARMAAAA
                     GMPVVPHMSGGGLGYLDVVHFASFTPNIGPFMEFKGNTDLPAHCPTSSLKVERGLVRV
                     PTGSGCGITIDPAFVKQARVVAAGP"
     gene            complement(1442..2893)
                     /locus_tag="VFB80_09605"
     CDS             complement(1442..2893)
                     /locus_tag="VFB80_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN34064.1"
                     /translation="MRFPLTLCAAAILAYWVHSALAAEPLLLNNAEFAQWQDRAPVGW
                     SVSVGAKSGGDVPSRLQKADGGGIELSGDAATGQWRVVSQKVAIGKEGGLRLQFEAST
                     ADLQRERGQFDNCYVGLNVVDAGGKRATFAFRNLFETKWAPGQVVVNLPASAAAVEVM
                     LVLSKSGKLQVRNLRLEQLAPADSFDVLAAELDRYYSFFALKKFDWRARAAVYEAQGR
                     AAKTPDEFIAAVQPLLAELKDLHVVIVADGGKETPTYISRADRNFDARTIAGKLKDVK
                     QIGRMGFTARTPEGYGYAALGTLAADEKTTAEMLAAVDALLDAKGLILDIRVNGGGQE
                     GVAQQIVSRLIGQPLAYAKNQFRGGAAHDDLVTLGTRQVAPRLDQPFRGPVVGLIGPG
                     CVSSGEGMALMLKALPNATLIGQNTRGASGNPQPVALPNGLTVRYSTWVPLDMEGQPF
                     EGTGIQPATRIDDDSTGVKGLQAAIEHLNQATK"
BASE COUNT          488 a         1000 c          952 g          518 t
ORIGIN      
        1 agcagggcat agcggacgcc ctcgcggacc gccagcagcg agagcacgcc ggtcagggcc
       61 acggccgccg cgttccacag catcagccgc gcccggagcg atcgcagcgg cagcagcaag
      121 cgttccagcg gcataaccgc attctaactg cagggcaggc aaactgacga gcaagactaa
      181 ggcccagcgg cgacgactcg ggcctgcttc acgaaggccg ggtcgatcgt gatcccgcag
      241 ccgcttccgg tgggcacgcg gacgaggcca cgctcgactt tcagtgacga cgtagggcaa
      301 tgtgcgggca gatcggtgtt ccccttgaac tccatgaacg ggccgatgtt gggcgtgaac
      361 gaggcgaagt ggaccacgtc gagatagccc aggccgccgc ccgacatgtg cgggacgacg
      421 ggcatgccgg cagctgcagc catgcgggcg acctgcgtgg cgcggatgaa gccgccgccg
      481 taatgcaggt cgggctgcac gatgtccagg ccccgctcgg caaccgccca ccgccagcga
      541 gccatactga attcctgctc gccgccggca acgggtatcg tgagcgcgtc ggcgacttcc
      601 ttggtgtccc acaggtcgtc gaacgggcag ggctcttcgt agaagccgta gccgtgctgc
      661 tccatgaggc ggccgatgcg gatcgcctct ttggcgtcat aggagctgtt cgagtcggca
      721 tagagcgtca gcgcgtcgcc gaacgtcttg cgaacgagcg ggatcagggc ctcggtgcgc
      781 ccggggagcg aatcgacgtt cttgttcatc cgcccgccga ggcggaactt gatcgccttg
      841 gccccggagc cggcgacgag cttttgcaga tactcgacct cggcctcagg cgtattgccg
      901 cggttgccgc tggcgtaata gacggcaatc tcgtcgcgcg agcggccacc gaagaaatcg
      961 gccagcggcc ggccggccgt ctggcccatc agctccagga gggccatctc gatcgccgcg
     1021 acgccgggcc acagcgcgat cccctgcagc ttgtagttgc tgctgtggcg gtagacgtcc
     1081 cagagcagcg actccagctt ccgcgcatcc tgcttcaaga agaccggcac aatgttcttc
     1141 aggaagatcg gataggtctg cgccatccgc tcaggattcg gcacggttac aacttccacg
     1201 cccgccgccg agcgtgtccg caccaggtcg ttccgtccat tcacgagcaa ctcgaccgac
     1261 tccacgatta ctggctcttt cacgaagtca gtcgtcagca cgggctggtt gagaatccgg
     1321 tcgagttccg cccgcggcac tcgcagggca tctgccgcgc gtgcccgcgc gacaagcgca
     1381 caagagccgg cggccgcggc cgccagcaac gcccgcctgg aagtcttcat caaccggctc
     1441 cttatttcgt ggcctggttc aagtgctcga tcgcggcctg gagtcccttg acgcccgttg
     1501 agtcgtcgtc gatccgcgtc gccggctgaa tgcccgtccc ttcgaatggc tggccttcca
     1561 tgtccagtgg aacccacgtc gaatagcgta cggtcaggcc gttaggcagg gctaccggct
     1621 gcggattgcc gctggccccg cgcgtattct ggccgatcag cgtggcattt ggcagggcct
     1681 tgagcatcag cgccattcct tcgccgctgc tgacgcagcc cggcccgatc aggccgacga
     1741 ccggtcctcg gaacggctgg tccaacctcg gcgcgacttg ccgcgtgccg agcgtgacga
     1801 ggtcgtcgtg tgctgcgccg ccgcggaact ggtttttggc atacgccagc ggctgtccga
     1861 tcagacgcga gactatctgt tgggcgactc cttcttgccc gccgccgttg acgcggatgt
     1921 cgaggatcag ccccttggcg tccagcagag cgtccacggc ggccagcatc tcggcggtgg
     1981 tcttctcatc ggccgccagc gtgcccaggg cggcgtagcc gtatccttcc ggtgtccgcg
     2041 cggtgaaccc catgcggccg atctgcttga cgtccttgag cttgccggcg atcgtccggg
     2101 cgtcgaagtt gcgatcggcc cgggaaatat aggtgggcgt ttccttgccg ccgtccgcca
     2161 cgatgaccac gtgcaggtcc ttcagttctg cgagcagcgg ctgcacggcg gcaataaact
     2221 cgtccggcgt cttggccgcg cggccctggg cttcatacac ggcggcccgc gcgcgccagt
     2281 cgaacttctt gagggcgaag aagctgtagt agcggtcgag ctccgccgcg agtacgtcga
     2341 agctgtcggc cggagcaagc tgctccagac gaaggttgcg gacctgcagc ttgccgctct
     2401 tggagaggac cagcatcact tcgaccgctg cggccgacgc cggcagattc acgacgacct
     2461 ggcccggcgc ccatttcgtc tcgaacagat tgcggaaggc gaacgtggcc cttttgccgc
     2521 cggcatcaac gacgttgagg ccgacgtagc agttgtcgaa ctggccgcgc tcgcgctgca
     2581 aatcggccgt gctggcctcg aattgcaggc gcagcccgcc ctccttgccg atggccactt
     2641 tctgcgagac gacgcgccac tggcccgttg ccgcatcgcc gctcagctcg atcccaccgc
     2701 cgtccgcctt ctgcaaacgc gacgggacat cgccgccaga tttggctcct accgacacac
     2761 tccagccgac gggtgctcgg tcctgccact gggcgaactc ggcgttattt aataggagcg
     2821 gctccgcggc cagcgcgctg tgcacccaat acgcgagtat cgcggcagca cagagagtca
     2881 gcgggaatcg catgtcactt gtcctcaatc gggatgacgg cgtctggtcc tcgatgcaag
     2941 cggcgaatca ctttccct
//
LOCUS       DASRJB010000365         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1793767, whole genome shotgun sequence.
ACCESSION   DASRJB010000365 DASRJB010000000
VERSION     DASRJB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793767"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="metK"
                     /locus_tag="VFB80_09610"
     CDS             <1..742
                     /gene="metK"
                     /locus_tag="VFB80_09610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZN34065.1"
                     /translation="LNRLTRARQSGEVDWLRPDSKSQVTVEYDGDRAVRIDAVVVSTQ
                     HSPKVTQDQIKEYVINKVVKPELPKELVSGDIKYYINPTGNFVVGGPNGDCGLTGRKI
                     IVDSYGGWGRHGGGAFSGKDPTKVDRSAAYMARHVAKNIVAAGLAERCEVQLAYAIGV
                     AEPVSVNVDTFGTGRVDDERLCEVVRSFFSLTPSGIIKYLDLRRPIYRKTAAGGHFGR
                     SEPEFSWENTARAPQLAEAAGSLAGAAR"
     gene            800..1573
                     /locus_tag="VFB80_09615"
     CDS             800..1573
                     /locus_tag="VFB80_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34066.1"
                     /translation="MLPGRTSERQLAELAAWTLLAALALVAITLVLRRMSGALIQPLG
                     LGGLAAVALSCCAAAAAIRRSLCPGGVVLGTQYSVLRKEFVICLAITVITISILAAVT
                     LKGTPAVAVVPAWFLVAASEAASWLPAFRGHFAVARSAVARSAVRADEAELELPANLV
                     QQVTRVQDDAGGESVHAVLRAVVPAGDRQGAIHVAFCPPLVRVPQLTAHALDGEADVR
                     ITQAESFGARLEVKLPAIAAADQAVLVEVIGSVTAAQSA"
     gene            complement(1551..2639)
                     /locus_tag="VFB80_09620"
     CDS             complement(1551..2639)
                     /locus_tag="VFB80_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34067.1"
                     /translation="MPHSIQILVAALLLAAAGCGPSAPTAPPATAPAPPQTNTAADPV
                     QPTTAASPPSAAPSTAPPAAESGLAGLMARYLKSDEAGIWRPDEQAATELEKLAPTAA
                     QVLPLLANPRVEVRRSAAFYWLGSFDPAAPEQVAHFTKLLGDSDRTIRGIGLAAVKEL
                     PAADQLAALPQLAEMLDPAREDKADNRAAIARLLGSLRAQAAPGLPALLAAAKSDPDG
                     KVRAAGLAAIAQIAPAAEAIPPLTAGLADKDAAVKVVAAAQLRRLGPAAAPAAKELAA
                     ALSDADQRVREAATEALILIGAPAVEPLAGVLAGKSDEARKYALVALGRIGPPAKAAL
                     PGIEKCLKDGDAATSRLAAQALKQIGPP"
     gene            complement(2654..3568)
                     /locus_tag="VFB80_09625"
     CDS             complement(2654..3568)
                     /locus_tag="VFB80_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZN34068.1"
                     /translation="MFRRLRHLLEMIRFSHTLFALPFALLAAVMAWATPLATHRSPPT
                     FDWRQLLGILVCMVGARSAAMAFNRLVDRRIDAENPRTRSRHLPAGILSATSVWLFTL
                     ASAAVFVAGTLLFLPNRLPLLLSLPVLLFLLGYSYAKRFTALAHFWLGAALMLAPVCA
                     WIAIRGEVLLVQPADILPAVLLGAAVLAWVSGFDIIYACQDADFDRQARLRSVPALLG
                     VKSALRLAALCHLLALVLLAALPLICPQLALGWIYGTGVTAVAVLLVYEHWLVRPDDL
                     TRVNVAFFNINVIISLGLLVVGTVDLLL"
     gene            complement(3664..4701)
                     /locus_tag="VFB80_09630"
     CDS             complement(3664..4701)
                     /locus_tag="VFB80_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN34069.1"
                     /translation="MFLGVEIGGTKLQLGVGAGDGSELATLVRRDVDAARGAAGILEE
                     IERSATALIQKHKVQRIGFGFGGPCDAGRGTATKSHQVAGWENFPLVRWCQESLGKPA
                     VIGNDCDVAALAEARFGAGRGALGVLYVTVGTGIGGGLVFGGKLHGSGRPAVAEIGHL
                     RPGLNADLPEMTVESLAAGPAIAAAAVARMSGQVSRPLDTVRGPLEHQSRQQVRQRLA
                     DVQDTETEYVADLLQRAGGDAERLTARHVAQAAAEGNDIARQVLDHACQALGWAIAQA
                     ITLVAPEVVVIGGGVSLIGEPFFFGPVRAEVQRYVFPPLAGSFRIVPPLLGELAVVHG
                     AVALAAEQNSQ"
     gene            complement(4717..5448)
                     /locus_tag="VFB80_09635"
     CDS             complement(4717..5448)
                     /locus_tag="VFB80_09635"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HZN34070.1"
                     /translation="MTTAATYSPPASHEESLAPYRAVCRSAVLSLVLALISLPLVAMA
                     VVSCLFRFGDAVQVGLVGGALALPAALLGWSGWRTVRRYPDEYTGGGLARFGFIAGLA
                     LLLVGFSAATFTYATEVPDGYERIGFWDLALDPDRPDLWYSPKAEQLEGKRIFIKGYM
                     HPGVATRGKVNNFILVKDLGTCCFGGQPQPTHMIAVYIPNAVDRPSYSTQTMKLAGTF
                     HLVKTPSSGLGVEGVLYHLQADQVR"
     gene            complement(5455..>6013)
                     /locus_tag="VFB80_09640"
     CDS             complement(5455..>6013)
                     /locus_tag="VFB80_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34071.1"
                     /translation="ELADLVIPDLAKFEDWEVMDKLFDLFKNANEKNSWVRVPVINYL
                     RTCPLPKAKTLLQECEKIDPAAVKRANTFFPGAPSAAPAPAAEKTSRSQPAPAAAGST
                     LAMRGEPDGSPGQSQSVGGGPPVPPEDNGDELLAAANQGAERAAAIRHSSLPAANLWA
                     VIGVPWAVGIAILLVQWSVLKSAAR"
BASE COUNT          969 a         2021 c         2125 g          898 t
ORIGIN      
        1 tctcaaccgg ctaacgcggg cgcgacagtc gggcgaagtc gattggctgc ggcccgacag
       61 caagagccag gtcacggtcg aatacgacgg cgaccgggcc gtgcggatcg acgccgtggt
      121 cgtcagcacg cagcattcgc cgaaagtgac gcaggatcag atcaaggagt acgtgatcaa
      181 caaggtggtc aagcccgagc tgcccaagga gttggtcagc ggcgacatca agtactacat
      241 caatccgacg ggcaactttg tcgtcggcgg tcccaacggc gactgcggct tgaccgggcg
      301 gaagatcatc gtggatagct acggcggctg ggggcggcac ggcggcggcg cgttcagcgg
      361 caaggacccg accaaggtcg atcgcagcgc ggcctatatg gcccgccacg tggccaagaa
      421 tatcgtcgcc gccggcctgg ccgagcggtg cgaggtgcag ctggcctacg cgatcggcgt
      481 cgccgaaccg gtgagcgtca acgtcgatac cttcggcacc ggccgcgtgg acgacgagcg
      541 gctgtgcgaa gtcgtgcggt cgttcttctc gctgacgccg agcgggatca tcaagtatct
      601 cgacctgcgg cggcccatct atcgcaagac ggctgctggc ggtcatttcg gccgctccga
      661 gcccgaattc agctgggaaa acactgcccg cgctccgcaa ctggccgaag ccgccggctc
      721 gctggccggc gcggcgcgat gacttggagc gggcctctgg cctgccgcta ataccatgcg
      781 ctccacggcg ggagccctga tgctgccagg acgaaccagc gagcggcagt tggcagagct
      841 cgccgcctgg acgttgctgg ctgcgctggc gctggtggca atcacgctcg tcctccgccg
      901 catgagcggc gcactgatcc agccgttggg tttgggcggg ctggcggccg tggctttgtc
      961 gtgctgcgcg gcggcggccg ccatccgccg gtcgctgtgc ccaggcggcg tcgtacttgg
     1021 tactcagtac tcagtacttc gtaaggaatt cgtgatttgt ctggcgatca cagtcatcac
     1081 gatttcgatc ctggctgccg tgacgcttaa gggcacaccg gccgtagccg tcgtgccggc
     1141 ctggttcctg gtcgcggcga gcgaagccgc gagctggctg ccggcctttc gcgggcactt
     1201 tgcagtcgcc cgaagtgcag tcgcccgaag tgcagtcagg gctgacgaag ccgagctgga
     1261 gctgcccgcg aacctggttc agcaagtgac gcgagtgcag gacgacgcgg gcggcgagtc
     1321 ggtgcatgcg gtgctccggg ccgtagttcc ggccggcgat cggcagggag cgattcacgt
     1381 cgcgttctgc ccgccgctgg tgcgagtgcc gcagctgacg gcgcacgcgc tggacggcga
     1441 agccgacgtg cggatcacgc aggccgagtc cttcggcgcg cggctcgaag tcaagttgcc
     1501 ggcgattgcg gcggcggatc aggccgtgct ggtcgaggtc attggctcgg tcacggcggc
     1561 ccaatctgct tgagggcctg ggccgccagc cggctcgtgg ccgcgtcgcc gtccttcagg
     1621 catttttcga tgcccggcag ggctgctttt gccggcgggc cgattctgcc gagggcgacg
     1681 agggcgtact tgcgggcctc gtcgctcttg ccggccagca cgccggccag cggctcgacg
     1741 gccggcgcgc cgatcaggat cagcgcctcg gtcgcggctt cgcggacgcg ctggtcggca
     1801 tcggacaatg cggcagcgag ctcctttgcg gccggcgcgg cagccgggcc cagccgccgc
     1861 agctgagccg cggccacgac ctttacggct gcgtccttgt cggccaggcc cgcggtcagc
     1921 ggcgggatgg cctcggcggc gggcgcgatc tgcgcgatcg ccgccagtcc cgcggcccgg
     1981 accttgccgt cgggatctga cttggcggca gccagcagcg ccggcaatcc cggtgcggcc
     2041 tgcgcacgga gcgagcccaa gagccgggcg atggccgccc ggttatcggc cttgtcttcg
     2101 cgggccggat cgagcatctc ggccagttgg ggcagcgcgg cgagctggtc ggccgccggc
     2161 agctccttga cggccgccag tccgatgccg cgaatcgtgc ggtcgctgtc ccccagcagt
     2221 ttcgtgaagt gggcgacctg ctcgggcgca gcggggtcga acgaacccag ccaatagaac
     2281 gctgccgagc ggcggacttc aacccgcgga ttcgctagca gcggcaacac ctgggcggcg
     2341 gtgggtgcaa gcttttccag ctccgtggct gcctgctcgt cgggccgcca gatgcccgcc
     2401 tcgtcgctct tcaggtagcg agccatgagc ccggccagcc cgctctccgc ggcgggcggc
     2461 gcagtggacg gcgcggctga gggcggcgac gccgctgtcg tcggctgaac gggatcggcg
     2521 gccgtgttgg tctgcggtgg agcgggcgca gtcgcaggcg gcgcggtcgg cgcgcttggg
     2581 ccgcagccgg cggcagccag cagcaaagcg gcgaccagaa tctgaatcga gtgcggcatg
     2641 cagcatccgt gcgtcagagg agcagatcga ccgtgccgac aaccagcagg ccgaggctga
     2701 tgatgacatt gatattgaag aacgccacgt tgactcgcgt caagtcgtcc ggccggacaa
     2761 gccagtgttc atagacgagc agcacggcca cggcggtcac tcctgtgccg tatatccacc
     2821 ccagcgcgag ttgcgggcag atcagcggca gcgcggccag cagcacgagc gccagcagat
     2881 gacagagagc ggccagtcgc agagcactct taacgcccaa cagcgcggga acgctgcgga
     2941 ggcgtgcctg ccggtcgaag tcggcgtcct ggcaagcata gatgatgtcg aaccccgaaa
     3001 cccaggccag caccgccgcg cccagcagca ccgcgggcag gatgtcggcc ggctggacca
     3061 gaagcacttc gccgcggatc gcgatccagg cacagaccgg cgcgagcatc agcgccgcgc
     3121 cgagccagaa gtgggccagc gccgtgaaac gcttggcata gctgtagccg agcaggaaca
     3181 gcaacaccgg cagcgacagc agcagcggca gccggttcgg cagaaacagg agtgtgccgg
     3241 cgacgaacac ggccgccgac gccagcgtga agagccagac gctggtcgcc gagaggattc
     3301 cggctggcag gtgccgcgac ctggtccgcg gattctcggc gtcgatccgc cggtccacca
     3361 gccgattgaa ggccatcgcc gcgctgcgtg cgccgaccat gcagacgagg atgcccagca
     3421 gttgacgcca gtcaaaggtc ggtggagagc ggtgagtggc cagtggtgtg gcccaggcca
     3481 tgaccgccgc caagagcgca aaggggagcg cgaacagcgt atggctgaag cggatcatct
     3541 ccaggaggtg gcggagcctt cgaaacatgt gcctttagcc gggagcaaac ctgctcgcgg
     3601 agcgagcgcg tgaccggtcc attctaaaga ccgcaaatgg aaccatcgcg tcacattcga
     3661 ttttcactgg ctgttctgct ctgccgccag ggcgacggcc ccgtggacca cggcgagctc
     3721 gcccagcagc ggcggaacga tgcggaacga cccggccagg ggcgggaaga cgtagcgctg
     3781 gacttcagcc cgcacggggc cgaagaagaa cggctcgcca atcagcgaga cgcccccgcc
     3841 gatgaccacg acttccgggg cgacgagcgt gatcgcctgg gcgatcgccc agcccagcgc
     3901 ctggcaggcg tggtcgagca cttgccgggc gatgtcgttc ccttcggccg cggcctgggc
     3961 gacgtgccgc gccgtcagcc gctcggcgtc gccgccggct cgctggagca aatcggccac
     4021 gtactccgtc tcggtgtcct gcacgtcggc cagccgctgg cggacttgct ggcggctctg
     4081 atgctcgagc ggcccgcgga ccgtatcgag cggccgcgag acctggcccg acatgcgggc
     4141 gacggcggcc gcggcgatcg cggggcccgc ggcgagcgat tcgacggtca tctccggcag
     4201 atcggcgttg agccccggcc gcaggtggcc gatctcggct accgcgggcc ggccgctgcc
     4261 gtgcagcttg cctccgaaga ccagcccgcc gccgatgccg gtgcccacgg tgacgtagag
     4321 cacgcccagc gcgccgcggc cggcgccgaa gcgggcttcc gccagcgcgg cgacgtcgca
     4381 gtcgttgccg attaccgcgg gcttgcccag cgattcctgg caccagcgga ccagcggaaa
     4441 attctcccag ccggcgacct ggtgactctt ggtcgcggtg ccgcggccgg cgtcgcaagg
     4501 tccgccgaag ccaaatccga tccgctggac cttgtgcttc tggatcaggg ccgtggccga
     4561 gcgttcgatc tcttcgagga tgcccgccgc cccgcgggcc gcatcgacgt cgcgccggac
     4621 cagggtcgcc aactcgctgc catcccccgc gccgacgccc agctgcagtt tcgtgccgcc
     4681 gatctcgacg cccaggaaca tgcccgcgcc ccggagctac cgcacctggt cggcctggag
     4741 gtgatacagc acgccttcga ctcccagacc gctgctcggc gtttttacga gatggaacgt
     4801 gccggccagc ttcattgtct gcgtcgagta gctgggccga tccacagcgt tgggaatgta
     4861 gacggcgatc atgtgcgtcg gctgcggctg cccgccgaag cagcaggtgc cgaggtcctt
     4921 gaccaggatg aagttgttga ccttgccgcg ggtggcgacg cccggatgca tgtagccttt
     4981 gatgaagatc cgcttgccct ccagctgctc ggccttgggc gagtaccaca aatccggccg
     5041 atcggggtcg agcgccaaat cccagaagcc gatccgctcg tagccgtcgg gcacttccgt
     5101 cgcataggtg aaggtcgccg cgctgaagcc caccagcaag agcgccaaac cggcgatgaa
     5161 gccgaaccgg gccaggccgc caccggtgta ttcgtcggga tagcgccgca cggtccgcca
     5221 accgctccag ccgagcagcg ccgcgggcaa ggccagcgcg ccgcccacca ggccgacttg
     5281 caccgcgtcg ccgaagcgga acaggcacga gaccaccgcc atcgcgacca gcggcaggct
     5341 gatcagggcc agcacgagcg aaagcaccgc cgagcggcag accgcgcggt acggggcgag
     5401 gctctcttcg tggctggcgg gtgggctata ggtcgcggcc gttgtcatgt cacgttatcg
     5461 ggcggcgctc ttgagcaccg accactggac cagaagaatc gcaatcccca ccgcccatgg
     5521 tacgccgatc acggcccaca ggttcgccgc cggcagcgaa ctgtggcgaa tcgcggctgc
     5581 ccgctcggcc ccctgattgg ccgccgcgag caattcgtcg ccgttatcct ccggtggaac
     5641 gggaggcccg ccgccgaccg actgcgactg gccgggcgag ccgtcgggct cgccgcgcat
     5701 ggccagtgtc gagcctgccg cggcgggtgc gggctgcgag cgggaggtct tctcggcagc
     5761 cggagcaggc gccgcggaag gcgctccggg gaagaacgta ttggcccgct tcacggccgc
     5821 gggatcgatc ttctcgcact cctggagcag ggtcttggcc ttgggcagcg ggcaggttcg
     5881 caggtaattg atcaccggca cccggaccca gctgttcttc tcgttggcgt tcttgaagag
     5941 gtcgaagagc ttatccatca cttcccaatc ctcgaacttg gcgaggtcgg gaatcaccag
     6001 gtcggccagc tcc
//
LOCUS       DASRJB010000366         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_385697, whole genome shotgun sequence.
ACCESSION   DASRJB010000366 DASRJB010000000
VERSION     DASRJB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385697"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1751)
                     /locus_tag="VFB80_09645"
     CDS             complement(456..1751)
                     /locus_tag="VFB80_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZN34072.1"
                     /translation="MGRYLSLLPLVFVIALGGLAAWPRSVPQPPAAAATIRLAAPDHA
                     QVAANPPEHPVANAIFHVAPGARPPIRLCQEIIATPCDGPCQPPIFGVDCASGPPCGE
                     AHWDQWGPIPWQAFAQGEYVGPARLPHVPQYRLRPDDELEFVYRLTRAELSHSYQLEV
                     GDVIRIESLIDPTLNRELTVQPDGTVDLLLLGQVRVFRRSVEEVRGELNERYKKFYKL
                     TDINVTRLKTQTKLEDLRAAVDNRFFSGGQGKRVRVTPEGTVSLPAIGIVPAQGLTLD
                     EIKLEVDARYKAVVDGVEVTPILAQRAPRFVYVVGEVRNPGRFDLVAPTTAMQSIALA
                     GGWNVGGNLREVVVFRRADDWRLLATRLDIRGALYGERPAPSDEIWLRDSDIVVVPKS
                     PIRRLDDLIELYFTRGAYSAFPIFFNYSINGSPAVIAGP"
     gene            complement(1756..>2264)
                     /locus_tag="VFB80_09650"
     CDS             complement(1756..>2264)
                     /locus_tag="VFB80_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34073.1"
                     /translation="QLAYAAGGQPMASQVLFRLGKLQTALAAHDAAPQALHGPQAMVY
                     HQAALAADGSNFQAANELGVLLARYGQLQEAKLLLVRSVRIRPQVETWHNLAVVHRRL
                     GEVELARQADRERELLGQKSGGRTNAAASDAVRLVDARTFAAAAGGEAGWPASTAVRP
                     TAVNQQRR"
BASE COUNT          340 a          785 c          794 g          345 t
ORIGIN      
        1 ggctaaagaa accccgcagc agcgagccca ccgcgaggcc gccgagaaag ccgagcgccg
       61 ccgcctgcac atgctccgcc tcgaagaatg ccgcgaagcc cagcgcaagg gcctccccac
      121 cctcttcgtc ttcgaccccg aggacggccc cctgcccccg cggggaggct ggttctaggg
      181 tgagccccac gcagcaagta gggtgggcta tgcccaccac agcgccgcgg tgggcacagc
      241 ccaccctacg cgatggactt ctgggacccc agtttccact ttgcgttttg catttctcaa
      301 tttgcaattt gcaatctctc gtgatcgcca ggcgccgttt cacgcggcag ccgccccatg
      361 gcgccgggcg cttcgctgct gcaccccact gcctccgacg ctggcagcgt cggctacggc
      421 agccggcctg ggggccgggg ctaaacggcg ccgccctagg gtccggcgat cacggcgggc
      481 gagccgttga tcgagtagtt gaagaagatg ggaaacgcgg aataagcgcc gcgggtgaag
      541 tacagctcga tcaggtcgtc gagccggcgg atgggggact tgggaacgac gacgatgtcg
      601 ctgtcgcgga gccagatctc gtcgctgggt gcgggccgct cgccgtagag cgcgccgcga
      661 atgtccagcc gcgtggccaa gagccgccag tcgtcggccc ggcggaagac gacgacctcg
      721 cggaggttgc cgccgacgtt ccagccgccg gccagggcga tcgactgcat cgcggtggtc
      781 ggtgcgacca ggtcgaagcg gccggggttg cgaacctcgc cgacgacgta gacgaatcgc
      841 ggggcccgct gggcgaggat cggcgtcacc tccacgccgt ccacgaccgc cttgtaacgg
      901 gcgtccacct cgagcttgat ttcatccagc gtcagcccct gggccgggac gatgccgatc
      961 gccggcagcg acacggtgcc ttccggcgtc acccggactc gcttgccctg gccgccgctg
     1021 aaaaagcggt tatcgacggc cgctcgcagg tcctcgagct tcgtctgcgt cttgagccgc
     1081 gtgacgttga tgtcggtcag cttgtagaac ttcttgtacc gctcgttgag ctcgccgcgg
     1141 acttcttcca cgctgcggcg aaacacgcgg acctggccca gcagcaacag atcgaccgtg
     1201 ccatcgggct gcacggtcag ctcgcggttg agcgtgggat cgatcagcga ctcgatgcgg
     1261 atcacgtcgc ccacttcgag ctggtagctg tgcgagagct cggcccgcgt caggcgatag
     1321 acgaactcca gctcgtcgtc cggacgcagg cgatactgcg gcacgtgcgg cagccgggcc
     1381 ggcccgacgt attccccctg ggcgaaggcc tgccagggaa tcggccccca ctggtcccag
     1441 tgggcctcgc cgcagggcgg gccgctggcg caatcgacgc caaagatcgg cggctggcac
     1501 ggcccgtcgc acggcgtcgc gatgatttcc tggcagagcc tgatcggcgg ccgcgcgccc
     1561 ggtgcgacgt ggaagatcgc gttcgcgacg ggatgctctg gcggatttgc ggcgacctgg
     1621 gcgtggtcgg gcgcggcgag ccgaatcgtg gcagcagccg cgggcggctg cggcactgac
     1681 cggggccagg cagccagccc gcctagcgcg atgacaaata cgagcggcag cagcgacaag
     1741 tatcggccca tggcgctatc tccgctgctg gttgacggcc gtgggtcgga cggcggtgct
     1801 cgcgggccag ccggcctcgc cgccggccgc ggccgcgaac gtccgcgcat caaccagccg
     1861 cacggcatcg ctggcggcgg cgttcgtgcg gccgccggac ttctggccga gcagctcgcg
     1921 ctcgcggtcg gcctggcggg ccagctcgac ttcgcccaag cggcgatgta cgaccgccag
     1981 gttgtgccac gtctcgacct gcgggcggat cctcacactt cggaccagca gcagcttcgc
     2041 ctcctgcagc tggccgtagc gggccagcag tacgcccagc tcgttggccg cctggaagtt
     2101 gctgccgtcg gcggccagcg ccgcctggtg atagaccatc gcttgcggcc cgtgcagcgc
     2161 ctgcggggcg gcatcgtggg ccgccagcgc cgtttgcagt ttgccgagtc ggaacagcac
     2221 ttgcgaggcc atgggctgcc cgccggcggc gtaggcgagc tgcg
//
LOCUS       DASRJB010000367         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3484348, whole genome shotgun sequence.
ACCESSION   DASRJB010000367 DASRJB010000000
VERSION     DASRJB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484348"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..472
                     /locus_tag="VFB80_09655"
     CDS             212..472
                     /locus_tag="VFB80_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZN34074.1"
                     /translation="MQLAFVVGTATSTIKHATMRGQKLLVVQPLMADGKAADGDPQVA
                     VDAVGAGQGQRVMITSDGKYMREILKTEATPVRWSVIGISDE"
     gene            469..720
                     /locus_tag="VFB80_09660"
     CDS             469..720
                     /locus_tag="VFB80_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34075.1"
                     /translation="MTATPEQLEWIVQEVIRRLRQGDGTPHAAGGELRLSDRVVTLAA
                     LKDRLNNVSRVSVPAKAVVTPAVRDELKQRGVDLVRQTT"
     gene            866..1144
                     /locus_tag="VFB80_09665"
     CDS             866..1144
                     /locus_tag="VFB80_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZN34076.1"
                     /translation="MRIAKVVGTVTLNRCHPSFNGARLRLVVPLSLAELRGEQQPQAD
                     EMVAWDDNGAGLGSLVAISEGAEASQPFRPELKPVDAYAAAILDEVNT"
     gene            1220..2086
                     /locus_tag="VFB80_09670"
     CDS             1220..2086
                     /locus_tag="VFB80_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN34077.1"
                     /translation="MANLHKLKQDICEIGRRIYAKGFAAANDGNITVRTGENEVLCTP
                     TMHCKGFLKPEDICLIDMTGKQLAGIKKRSSEALLHLEIYRQRPDVKSVVHCHPPHAC
                     AFAIAREPIPQCVLPEVEVFLGDVPITRYETPGGQGFADTVIPFVQKTNVIILANHGT
                     VSYGENVERAYWWTEILDAYCRMLLLSKQLGRVNFFDETKERELLDLKQKWGWSDPRN
                     TKEYENCDICANDIFRDSWQKSGVERKAFPAPPPMGPNAAKANGAAAGGMDQEALVQM
                     ITQRVMEELAKH"
     gene            2190..3137
                     /locus_tag="VFB80_09675"
     CDS             2190..3137
                     /locus_tag="VFB80_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate dehydrogenase family protein"
                     /protein_id="HZN34078.1"
                     /translation="MKVSIIGGGGLVGSSAGFALQCGGVVREIALLDVNQELASGQAL
                     DLLHGGPSVADQVIVAGGYEHVPTSDVVCITAGLRRKPDESRLDLINRNTDLFLGILA
                     EVAKTGVKKDAVVLVVSNPVDILTYVAADRLDLPTAQVLGLGTQLDTIRFRSLIAQEL
                     KAAPTQVSATILGEHGDSMVPIWSSAAIAGLPIDKYPGWSNRLADELFTRTKGSGAEV
                     IKKKTGAGFAVGIAIRDVIHSIALDQRRVLPVSTVQNGCYDIRDVALSVPTVVGRGGC
                     LARHAIDLWPKEVQLLRKSGLVLRQTLEQVLSRIGKSKK"
     gene            complement(3211..4029)
                     /locus_tag="VFB80_09680"
     CDS             complement(3211..4029)
                     /locus_tag="VFB80_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34079.1"
                     /translation="MSDVPQRRWFRFSLRTLFVLVTVLCCWLAWESSVVRARKNLLRE
                     LKVNPTYTVTTAAEWARRYPTGFLGQPPKAVPFVRRCLGDEAIQEIGYYTYRKEKSQL
                     NLPRIARTFPEAELKEDHPPLEPCHPGCFPRGTFVETPGGPRAIETIRAGEELIAILP
                     SGEMTIATVQSVFVTTNRLWRIETAAGVFFTTKQQPLCLAADRTLPAGELEPGQSILR
                     FEGGATCEANVVSAAPTDRIETVFNLVLGDCERFIAGGYLARSKPPALAASPED"
BASE COUNT          840 a         1404 c         1440 g          738 t
ORIGIN      
        1 atggcctcgc tcggcctcgg cggcgagggc tacctgtcgt tctccatcgc cggtcccacg
       61 ggcgagggcg tgacgacgcc gctgacgttc acccgcgagc ggcgctgctc gttgatcgat
      121 gatttgtgca ttttgggaaa acggtaatca aagccgcgac cattggtcgc ggtgtattgc
      181 agaccgcgac caatggtcgc ggctttgaga catgcaactc gcctttgtag tcggcaccgc
      241 gacgtcaacc atcaagcacg cgaccatgcg cgggcagaag ctgcttgtcg ttcagccgct
      301 gatggcggac ggcaaggcag ccgacggtga cccgcaggtg gccgtggatg cggtcggcgc
      361 gggccagggg cagcgggtga tgattaccag cgacggcaaa tacatgcggg aaatcctcaa
      421 gaccgaggcc acgccggtac ggtggtcggt gatcgggatc agcgacgaat gaccgccacg
      481 cccgaacaac tcgagtggat cgtgcaggag gtcatccgcc gcctgcggca aggcgacggc
      541 actccgcacg cggccggcgg cgagttgcgg ctgagcgacc gcgtcgtcac gctggcggcc
      601 ctgaaggatc ggctgaacaa cgtttcccgc gtcagcgtgc cggccaaggc cgtcgtgact
      661 ccagcggttc gcgacgagct caagcagcga ggcgttgacc tggttcgcca aacaacgtga
      721 agctggtact ggccatttcc ttgaaagaac gcaaaggcgc taagcatcgc aaagccgcaa
      781 agaggacaaa gcaatgaatc cgtccctttc tgcctctccg cgtccttggc gttcctccgc
      841 gtctttgcgt tgaaaagaaa aaggtatgcg aatcgccaaa gtcgtcggca ccgtcacgct
      901 caatcgctgc caccccagtt tcaacggggc tcgcctgcgg ctggtcgtcc cgctctcgct
      961 ggccgaattg cggggcgagc agcagccgca ggccgacgag atggtcgcct gggacgacaa
     1021 cggcgccggc ctgggcagcc tcgtcgcgat cagcgaaggc gccgaagcgt cccagccgtt
     1081 ccggcccgag ctgaaaccgg tcgatgcgta cgcggcggcg atcctcgatg aagtgaatac
     1141 atagtggcta gtgatcaatc accagccgct aaccacgaac actagccacc agcactagcc
     1201 actagccact ccagcctcca tggccaacct ccacaagctc aaacaagaca tctgcgaaat
     1261 cggccggcgg atttatgcca agggcttcgc ggctgcgaac gacgggaaca tcaccgtccg
     1321 caccggcgaa aacgaagtcc tctgcacgcc gacgatgcac tgcaagggct tcctcaagcc
     1381 cgaggacatc tgcctgatcg acatgacggg caagcagctg gccgggatca agaagcggtc
     1441 gagcgaggcc ctcttgcacc tggagatcta ccggcagcgg cccgacgtga agagcgtcgt
     1501 gcactgccac ccgccgcacg cctgcgcgtt cgcgatcgcc cgcgagccga tcccgcagtg
     1561 cgtcctgccg gaagtcgagg tgtttctcgg cgacgtgccg atcaccagat acgaaacgcc
     1621 cggcggccag ggcttcgccg acacggtgat cccgttcgtg cagaagacga acgtgatcat
     1681 cctcgccaac cacggcacgg tcagctacgg cgagaacgtc gagcgggcct actggtggac
     1741 ggaaatcctc gacgcctatt gccggatgct gctgctcagc aagcagctgg ggcgcgtgaa
     1801 cttcttcgat gagacgaagg agcgcgagct gcttgatttg aagcagaagt ggggctggtc
     1861 cgatccgcga aacacgaagg aatacgagaa ctgcgacatc tgcgccaacg acatcttccg
     1921 cgacagctgg cagaaatcgg gcgtcgagcg caaagccttc ccagccccgc cgccgatggg
     1981 accgaacgcc gccaaggcca atggcgcagc ggccggcggg atggatcaag aggcgctcgt
     2041 gcagatgatt acgcagcgtg tgatggaaga actggcgaag cactagcgag tggctagtgc
     2101 tggtggctag tggctagtta tcagcaacag cagtcacatg cactagcctc tagccactag
     2161 ccaccagcac tctcgcaacc ctgaacccta tgaaagtttc catcatcggc ggcggcggac
     2221 tcgtgggctc cagcgccggc tttgcgctgc agtgcggcgg agtcgttcgc gagatagcgc
     2281 tcttggacgt caatcaggag ctggcgagcg gccaggcgct cgacctcttg cacggcgggc
     2341 cgagcgtggc cgaccaggtg atcgtcgccg gcggctacga acacgtgccc accagcgacg
     2401 tcgtctgcat taccgccggc ctgcggcgca agcccgacga gtcgcggctg gacctgatca
     2461 accgcaacac cgacctgttc ctcggtattc tcgcggaggt cgccaaaacg ggggtgaaaa
     2521 aggacgcggt cgtgctcgtc gtgagcaacc cggtcgatat cctcacctac gttgcggccg
     2581 accggctcga tctgccgacg gcgcaggtgc tgggcctggg cacgcagctc gacacgattc
     2641 gcttccgcag tctgatcgcc caggagctca aagccgcgcc gactcaggtc agcgcgacaa
     2701 tcctcggcga acacggcgac agcatggtgc cgatctggtc gagcgccgcc attgccggcc
     2761 tgccgatcga caagtatccc ggctggagca atcgcctggc cgacgagctg ttcacgcgga
     2821 ccaagggctc cggcgcggaa gtcatcaaaa agaagaccgg cgcgggcttc gccgtcggca
     2881 tcgccatccg cgacgtgatc cactcgatcg ccctcgacca gcgccgcgtc ctgcccgtct
     2941 cgaccgtcca gaacggctgc tacgatatcc gcgacgtggc cctcagcgtg cccaccgtcg
     3001 tcggccgcgg cggctgcctg gcgcggcacg ccatcgacct ctggccgaag gaagtgcagc
     3061 tcctccgcaa gagcggcctc gtcctgcggc agacgctcga gcaggtgctc agccgcatcg
     3121 gcaaatcgaa gaaatagcag ttgcggtggc ggaggatgtg gcggtggcgg ctgcggcggc
     3181 ggagggtgcg gcggctgcgg cagttagcat tcaatcttca gggctcgcgg ctaaggccgg
     3241 cggtttgctg cgggcgagat agccgcccgc gatgaaccgt tcgcagtcgc cgaggacgag
     3301 gttgaagacg gtttcgatgc gatcggttgg tgcggcggaa acgacattcg cttcgcacgt
     3361 cgcgccgccc tcgaaccgga ggatcgattg cccgggctcc aactcgcccg ccggcagcgt
     3421 ccgatccgcg gcgaggcaga gcggctgctg cttggtcgtg aagaagacgc cagccgcggt
     3481 ttcgatccgc cacaggcggt tggtggtgac gaagaccgat tgcacggtgg cgatggtcat
     3541 ttcgccgctg ggcaggatgg cgatcagctc ctcgccggcc cgaatggtct cgatcgctcg
     3601 cgggccgccg ggcgtctcga cgaacgtccc gcggggaaag cagcccggat ggcagggctc
     3661 caggggcgga tgatcctcct tgagctccgc ttcgggaaag gtccgcgcga tgcgcgggag
     3721 gttcagttgg gacttttctt tccgatacgt gtagtagccg atttcctgaa tggcctcgtc
     3781 gccgagacag cgccgcacga agggcaccgc cttgggcggc tgccccaaaa agccggtcgg
     3841 gtatcgccgg gcccattcgg cggccgtcgt caccgtatat gtcgggttga ccttgagttc
     3901 ccggagcagg ttcttccgcg cgcggacgac gctcgactcc caggccagcc agcaacatag
     3961 caccgtcacc agcacgaaca gcgtccgcag gctgaagcga aaccagcggc gctgcggaac
     4021 atcgctcatg gctgcaatcc cgggaatcgg tcgtgtgcgg gacagagacc caatcggagg
     4081 cgaatattcc cgtcgtttct ccaggaatta caatcttgct ggcaggcgga gtgggaaccg
     4141 aaggaaacga agaaagcgaa gagaggtagc ggaagagggg gcggctggca ttgagttgat
     4201 ctcgccttcg ttgccttcgt ttccttctgt ttgtattaca ctctgtcgtc cggcacctaa
     4261 gtcgtcaggt tcacctttat gcctatttac cagcttgtct gtcgggccgt tcggccgtgc
     4321 ttgtgtctcc tcgtcgccgc ggcgacccct gcgattggcc gcggccaatt gccggaggcc
     4381 gatcgggcgg ccttgctcca gaaactggac ggccgcgcga tg
//
LOCUS       DASRJB010000368         8596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3484593, whole genome shotgun sequence.
ACCESSION   DASRJB010000368 DASRJB010000000
VERSION     DASRJB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484593"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFB80_09685"
     CDS             <1..381
                     /locus_tag="VFB80_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN34080.1"
                     /translation="GGLPMGIEVQILDHGFKENYIKNNPNRKPDWFTTNGDVFPTQKA
                     KMKPFPPISPNGSRSFPKKELSKGAGEWNHYYIRAINGEVRLWVNGEEVSGGTNCEPA
                     SGYLCLESEGSPIEFRNLRVRVLP"
     gene            391..609
                     /locus_tag="VFB80_09690"
     CDS             391..609
                     /locus_tag="VFB80_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34081.1"
                     /translation="MQVEAASDESLIQTAQGKLPAADERRLKRLVGKSEEGRLTPLEQ
                     DDFIQLARRADRLEQTEMQPAHKNAGGF"
     gene            complement(647..1666)
                     /locus_tag="VFB80_09695"
     CDS             complement(647..1666)
                     /locus_tag="VFB80_09695"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HZN34082.1"
                     /translation="MLRETPFYCPECDEGFVMPAVRSRRGFLQALGGAAALAAVSPTL
                     RADDAPKAASKPAESLIKELYATLDASQKREVALPYDHDLNDGVTMRQRTFNASPNGT
                     KIGEKYTKAQQDLNLRILKAVLKDEEAYERLTRHGKWDSSGSFEGCGAVIFGEPGDKS
                     KFAWVFAGHHLTLRCDGNSQPGAAWGGPIYYGHSVNGYDKNNVYLYQTDAVQTVYDAL
                     DEKQRAKAHSTNNPGDTYPNGIKPTSPRHGIPLKELSAEQKATVERSMRVLLDPFRKE
                     DADEVMTLVKSNGGMDEVNIAFYKDDGASDKVRWHFWRLEGPGFVWNFRPLPHVHCFV
                     NVLQA"
     gene            1835..4303
                     /locus_tag="VFB80_09700"
     CDS             1835..4303
                     /locus_tag="VFB80_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34083.1"
                     /translation="MVRLALIAIFTGAAVVRAGETLPPVSQRFAAAASEQPDFQRHIV
                     PLLGTLGCNGRACHGSFQGQGGFRLSLFGYDFAADHAALTEGDEPRVDLKSPAGSLAL
                     QKPTLAIDHDGGQRMKLGSWQYQLLRQWIEAGAKPVAKDAAEFVALDVQPPEVLFTAK
                     GQTAALRVIARWSNGLAEDVTPLCRYQTRDESIATVDREGRVTAAGPGDSHIVVFYDN
                     GVVPVPVIQPLSQLTGERYPSAAAPTKIDELVLTKLKKLGLVPSERCTDEEFLRRVSL
                     DLTGTLPLAEEARAFAADSAEGKRAKKVEELLARPSYAAWWATRLGDWTGNGEATGPV
                     GGEQGLRRRFSDMWYRWLEHRIGQNVPYDQIVAGIVLATSRRPEQSFEEYCAEMSGYF
                     RKEGPADFAARETMPYFWSRRALGAVEEKARAFSYSFLGVRLECAQCHKHPFDQWTKQ
                     DFEQFAAFFSGIKYGAGKRDQYQSMKDAAGFKGLDEDSGDYKRKFVAALEKGDVLPFK
                     EVTIPQRSARERARPAATTNKAGRVITPRLLGGEEVLTQRYDDPRQPLMEWLRQDDNP
                     YFARALVNRVWANYFGVGIVEPPDDMNLANPPSNAALLDYLTSEFVAHQYDLKWLHRE
                     ILASETYQRSWRPNETNQLDERNFSRAILRRLPAEVAVDAVAVATAADEPATALVSDP
                     AQVKLRAIGQTSGYSGTRDEKLFAVYLFGKPAREIACDCERSGSPSLQQTLYMRNDEE
                     LFKLLDRKDGWLAELARAEKAGDKHSPEGLIDAAYLRSLSRRPTDAERQRALAHLAAA
                     DSPLAGLRDVFWALLNTKEFVVNH"
     gene            4372..5853
                     /locus_tag="VFB80_09705"
     CDS             4372..5853
                     /locus_tag="VFB80_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN34084.1"
                     /translation="MLRHFLHDLRTTFQRQARRPALVHRNRTWTYAELDSTARACAAY
                     LQSIGVAAGDRVALYTDQKLPLLVANLGVWLAGGVPLPLNPRFTREEMRYYLSDSGAI
                     AVIAGRDQQPLVEQLARELPQPPLVVPDSAVLAPPPATFREVTSEATDPSLILYSSGT
                     TGWPKGVVHTQANVASSLAALAACWRVTPDDVVVNVLPLFHIHGLAFATHLTWLAGGC
                     VRILDAFEPRETLAAIGQGTIFMAVPPIYYRLLDTPEFRGAASGWDRVRLFTCGSAPI
                     RTEVLPELQQILGPPIINRYGMSEAFVISSLPLDGPWPDGSVGLPLSGVELRVQHAEN
                     RAAAAREAGAVQLRGPNLFREYWNKPEATAAAFSGGWFDTGDLGQLDSAGMLTLVGRQ
                     HDLIITSGYNVYPQVVERVIGECPGVLECAVLGLPDKDRGERVAAAVVRGDASLDESR
                     LRNWWRDRLVHYQQPKSILFVESLPKNSLGKILRRELRERFGE"
     gene            5926..6141
                     /gene="thiS"
                     /locus_tag="VFB80_09710"
     CDS             5926..6141
                     /gene="thiS"
                     /locus_tag="VFB80_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZN34085.1"
                     /translation="MPATLVTVIVNGQSRELSAPVTVAELLRRLEIPSRGVAVEVNLA
                     IVPRWRHAEHTLADGDQLEIVTLVPGG"
     gene            6154..7014
                     /locus_tag="VFB80_09715"
     CDS             6154..7014
                     /locus_tag="VFB80_09715"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZN34086.1"
                     /translation="MSLAPPVAETAADAPLRIGQHTLRSRLIVGTGKYDTFQTMRQSL
                     ALSGCDCITVAVRRERMYDGRGQNILDALDLDRYTLLPNTAGCYSAADAVRVARMGRE
                     ILLSLENPGADWVKLECLGDAKTLLPDPVETLRATEMLVADGFAVLCYTSDDPVIARR
                     LKKAGAAAVMPAGSPIGSGQGVLNPNNIRIILEYLKAEDPSYPVIIDAGVGTASDVTA
                     AMELGVDAVLLNTGIAHARDPLGMASAMKAACEAGRLAWLSGRIPKRLYASASSPEEG
                     VISMRPYPAP"
     gene            7365..7565
                     /locus_tag="VFB80_09720"
     CDS             7365..7565
                     /locus_tag="VFB80_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34087.1"
                     /translation="MLKPIEKPAGRQPLVHHPSDVLDCFCRYEVTDRTWLDFDQEISQ
                     QLLELEFANRQFIRVKPQSSRR"
     gene            complement(7567..>8596)
                     /locus_tag="VFB80_09725"
     CDS             complement(7567..>8596)
                     /locus_tag="VFB80_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN34088.1"
                     /translation="PQLSRIKQELLKKAAEQRLDAAETQEKLAALEREFLRQGRTVYA
                     KPVLEQLVTSKMLYLEFDRQIRKNAKDKYAEARAGIDGKLRSFFEKQLETCRGVVPTA
                     KPEDLTALMNQVPVAGPLAALMHNHQLESMSELDAKLRTMGTSLEQQIVQFSEGTLGR
                     ETVNRNIDQKPTVTHQEMLDYYEQHLADFAVPAKARFEILTVKWANFPDKDAARDALA
                     MMGNQVIYGKPLSAVARELSQEPNAKQGGYYDWVQKGSLASQAIDAAVFSLPLERLSR
                     PIDDDTGCHIVRVKERQDAATLSFFDVQPKIREAIVLQKKREDQRKFLAELQKNTVVW
                     TIFDAPAP"
BASE COUNT         1470 a         2801 c         2839 g         1486 t
ORIGIN      
        1 ggcgggctgc cgatggggat cgaggtgcag attctcgacc acggcttcaa ggaaaactac
       61 atcaagaaca accccaaccg caaaccggac tggttcacca ccaacggcga tgtctttccg
      121 acgcaaaagg ccaagatgaa gccctttccg ccgatttcgc ccaacggcag ccgcagcttt
      181 cccaagaagg agctgagcaa gggggccggc gagtggaacc actactacat ccgcgcgatc
      241 aacggcgaag tgcggctgtg ggtcaacggc gaggaggttt ccggcggtac gaattgcgag
      301 ccagccagcg gctatctctg cctggagtcc gaaggctcgc cgatcgagtt tcgcaacctg
      361 cgggtgcggg tattgccgta ggaggcggat ttgcaagtcg aagccgccag cgacgagtcg
      421 ctgatccaaa ccgcccaggg caagcttcct gccgccgacg agcggcggct aaagcggctg
      481 gtcggcaaga gcgaggaagg ccggctgact ccgctcgaac aagacgattt catccagctt
      541 gcacggcggg cggatcggct agagcaaacc gagatgcagc cggcgcacaa aaacgccggc
      601 ggtttttagc cgccggcggc gatcattcaa cgcaatcgtt cgggcgttag gcctgcagca
      661 cgttcacaaa gcagtggacg tgcgggagcg ggcggaagtt ccagacgaag ccggggcctt
      721 ccagccgcca gaagtgccag cggaccttgt cgctggcgcc gtcgtccttg tagaaggcga
      781 tgttgacctc gtccatgccg ccgttgctct tcacgagcgt catgacctcg tcggcgtctt
      841 ccttgcggaa cgggtcgagc agcacccgca tcgacctttc gacggtggcc ttctgctccg
      901 cggacagctc cttgagcgga atgccgtgcc gcggactggt cggcttgatg ccgttggggt
      961 aggtgtcgcc gggattgttg gtggagtggg ccttggcccg ctgcttctcg tcgagggcgt
     1021 catacaccgt ctgcacggca tcggtctgat acaaatagac gttgttcttg tcgtagccgt
     1081 tgacgctgtg gccgtaatag atcggtccgc cccaggccgc gccgggttgc gagttgccgt
     1141 cgcagcggag cgtcaggtgg tggccggcaa agacccaggc gaatttcgac ttgtcgcctg
     1201 gctcgccgaa gatcacggcg ccgcagccct cgaacgagcc gctgctgtcc cacttgccgt
     1261 gacgggtcag ccgctcgtaa gcttcctcgt ctttcagcac ggccttcaag atccgcaggt
     1321 tcaggtcctg ctgggccttg gtgtatttct cgccgatttt cgtgccgttg ggcgacgcgt
     1381 tgaacgtccg ctgccgcatg gtcacgccgt cgttcaggtc gtgatcgtac ggcagggcga
     1441 cttccctttt ctgcgaggcg tcgagcgtgg catacagctc cttgatgagc gattcggccg
     1501 gcttggaggc ggccttcggg gcgtcatccg ctcgcagcgt cggactcacg gccgctagcg
     1561 ccgccgctcc acccagggcc tgcaagaatc cgcgccggct gcgaacggcc ggcatcacaa
     1621 agccttcgtc acattcgggg caatagaacg gggtttcgcg gagcatagaa gtctccagtc
     1681 cagtaaagga gagaggcgga aatcgccggg aaatctacga cgcatcgaac gccgccgata
     1741 ggttatagtg gatcgcgcgg ccgatggcaa acattttcgg acgctagcgc atcaggatca
     1801 agtagtgcgg caaacggtgt ccggaggagt gacgatggtt cgattggcgt tgatcgcgat
     1861 cttcaccggt gcggccgtgg tgcgggcggg cgagacgctg cccccggtga gccagcgctt
     1921 cgccgccgcg gccagcgagc agcccgactt tcagcggcac atcgtgcccc tcctgggcac
     1981 gctcggctgc aacggccggg cttgccacgg ctcgtttcag ggacagggcg ggttccgatt
     2041 gtcgctcttc ggttacgact tcgcggccga tcacgccgcg ctgaccgagg gggatgaacc
     2101 gcgcgtggat ttgaaaagtc cggcgggcag tttggccctt cagaagccga cgctggcgat
     2161 cgaccacgac ggcggccagc gaatgaagct gggcagctgg caataccaat tgttgcggca
     2221 gtggatcgag gcgggtgcga agccggtggc caaggacgca gcggagttcg tcgcgctcga
     2281 cgtgcagccg ccggaggtcc tgttcaccgc gaaaggccag acagcggcgc tgcgggtgat
     2341 cgcccgctgg tcgaacggct tggcggagga cgtgacaccg ctctgccgct atcagacccg
     2401 cgatgaatcg atcgccaccg tcgatcgaga agggcgcgtg acggcagccg gcccgggcga
     2461 ttcgcatatc gtcgtgttct acgacaacgg cgtcgtgccc gtgccggtga ttcagccact
     2521 atcgcagctg acgggcgagc gctatccgag cgcggcggcc ccgacgaaga tcgatgagct
     2581 ggtcttgacg aagctgaaga agctgggcct cgtgccatcg gagcgctgca ccgacgagga
     2641 gtttctccgc cgggtcagcc tcgatttgac gggcacgctg ccgctggccg aagaggcgcg
     2701 ggccttcgcc gccgattcgg ccgaaggcaa gcgagcaaag aaggtcgaag aattgctcgc
     2761 gcggccgagc tatgcggcct ggtgggcgac gcggctgggc gattggaccg gcaacggcga
     2821 agcgaccggc cccgtcggcg gcgagcaggg cctccgccgc cgcttcagcg acatgtggta
     2881 tcgctggctc gagcaccgca tcgggcagaa cgtgccttac gaccagatcg tcgccgggat
     2941 cgtgctggcg accagccgcc gaccggagca gagttttgaa gagtactgcg ccgagatgag
     3001 cggctacttc cgcaaggaag gcccggcaga cttcgccgcc cgcgagacga tgccctactt
     3061 ctggagtcgc cgggcacttg gagccgtaga agagaaggcc cgcgctttct cctattcgtt
     3121 cctgggcgtg cggctggagt gtgcccagtg ccacaagcat ccgtttgacc agtggaccaa
     3181 gcaggacttc gagcaattcg ccgcgttctt cagcggcatc aagtacggcg cgggcaaacg
     3241 ggaccagtat cagtcgatga aggacgccgc gggcttcaag ggtctcgatg aagactcggg
     3301 cgactacaag cgaaagttcg tcgccgccct ggaaaaaggg gacgtgctgc cgttcaagga
     3361 agtcacgatt ccgcagcgct cggcccgcga gcgagcccgg ccggctgcga cgaccaacaa
     3421 ggccggccgc gtcattacgc cgcggctctt gggcggcgaa gaggtgctga cgcagcggta
     3481 cgacgatccg cggcagccgc tgatggagtg gctgcggcag gacgacaatc cgtactttgc
     3541 ccgggcgctg gtcaaccgcg tgtgggccaa ctatttcggc gtcggcatcg tcgagccgcc
     3601 ggacgacatg aacctggcca acccgcccag caacgcggcc ttgctcgact atctcacgag
     3661 cgagttcgtc gcccaccagt acgacctgaa gtggctgcac cgcgaaattc tggccagcga
     3721 gacgtatcag cgcagctggc ggccgaacga gacgaaccag ctcgacgagc ggaacttcag
     3781 ccgcgcgatt ttgcggcgat tgccggcgga agtcgccgtc gatgcggtag cggtggcaac
     3841 cgccgcggac gagccggcca cggccttggt aagcgatccc gcgcaggtga agctgcgggc
     3901 catcggccag accagcggct acagcggcac gcgcgacgag aagctgttcg cggtctatct
     3961 gttcggcaag ccggcccgcg agatcgcctg cgactgcgag cgctcgggca gcccgagcct
     4021 gcagcagacg ctctacatgc ggaacgacga ggagctcttc aagctgctcg accgcaagga
     4081 cggctggctg gctgaattgg cccgcgcgga aaaggcgggc gacaagcatt caccggaagg
     4141 gctaatcgac gccgcatacc tgcgctcgct cagccgccgg ccgacggacg ccgagcggca
     4201 gcgggcgcta gcgcacctcg ccgccgcgga cagcccgctg gccggcctgc gcgacgtctt
     4261 ctgggcgctg ctcaacacca aagagttcgt cgtgaatcac taagccgtag agtaagcgtc
     4321 ctcgcttgct cacctgcgct ggcagaattc ggtgcacaag cgaggacgct tatgctgcgg
     4381 cacttcctcc acgacctgcg gacgacgttc cagcggcagg cccgccggcc cgcgctggtg
     4441 catcgcaacc gcacgtggac ctacgcggag ctcgactcga ctgcccgggc ctgcgcggcg
     4501 tatttgcagt cgatcggcgt tgcggccggc gaccgcgtcg cgctctacac cgaccagaag
     4561 ctgccgctgc tcgtcgccaa tctcggcgtc tggctggccg ggggcgtgcc gctgccgctg
     4621 aatccgcggt tcacgcgcga ggagatgcgg tattacctga gcgacagtgg cgcgatagcc
     4681 gtcatcgccg gccgtgacca gcagccgctg gtcgagcagc tggcccgcga gctgccgcag
     4741 ccgccgctgg tcgtgcccga ttccgcggtg ctcgcgccgc cgccggctac ctttcgcgaa
     4801 gtcacgagtg aagccaccga tcccagcctg atcctctaca gctcgggaac gaccggctgg
     4861 cccaagggtg tggtccatac gcaagccaac gtggccagca gcctcgcggc gctggccgcc
     4921 tgctggcgag tgacgccgga cgacgtggtg gtgaatgtgc tgcccctgtt tcacatccac
     4981 ggcctggcgt tcgccacgca cctgacgtgg ctcgccggcg gctgcgtccg gattctcgac
     5041 gccttcgagc cgcgcgagac gctggcggca atcggccagg ggacgatctt catggccgtc
     5101 ccgcccatct attaccggct gctggacacg cccgagtttc gcggcgcggc cagcggctgg
     5161 gatcgcgtgc ggctattcac ctgcggctcc gcgccgattc gcaccgaggt gctgccagag
     5221 cttcagcaaa tcctcggccc cccgatcatc aaccgctacg gcatgagcga ggcctttgtc
     5281 atcagcagcc tgccgctgga cggcccatgg cccgacggat cggtcgggct gccgctgagc
     5341 ggcgtcgagc tgcgcgtgca gcatgccgag aatcgcgctg cggcagcccg tgaagccgga
     5401 gcggtgcagc tccgcgggcc gaacctgttt cgcgaatact ggaacaagcc cgaggcgacc
     5461 gccgcggcgt tttccggcgg ctggttcgac accggcgacc tgggccagct cgattcggcg
     5521 gggatgctca cgctcgtcgg ccgccagcac gacctgatca tcaccagcgg ctacaacgtc
     5581 tatccgcaag tcgtcgagcg ggtgatcggc gagtgtccgg gcgtgctgga atgcgccgtc
     5641 ctgggcctgc ccgacaagga ccgcggcgag cgggtcgcgg cggccgtcgt ccgcggcgac
     5701 gcttcgctcg acgagtcgcg gctgcggaac tggtggcgcg atcggctggt ccactaccag
     5761 cagcccaaga gcatcctgtt cgtcgaatcg ctgcccaaga acagcctggg caaaatcttg
     5821 cggcgcgaac ttcgcgagcg gtttggcgag tagtcaattg tcactggtca tttgtcattt
     5881 gtcatttgcc gctaaaatgg cgagaggtat ctgtccctga aacctttgcc tgccacgctc
     5941 gtgaccgtca tcgtcaacgg ccagtcccgc gagctttccg cgccggtcac ggtcgcggag
     6001 ttgctgcgcc ggctggagat tcccagccgc ggggtcgcgg tggaagtgaa cctggccatc
     6061 gttccgcgct ggcggcacgc cgagcacacg ctcgccgacg gcgaccagct ggagattgtt
     6121 acgctcgtcc ccggcggcta acgcgccctc acgatgtcac ttgctccccc tgtcgccgaa
     6181 actgccgccg atgcaccgct ccgcatcggc cagcacaccc tccgcagccg gctgatcgtc
     6241 ggcaccggca aatacgacac gttccagacg atgcggcagt cgctggccct ttccggctgc
     6301 gactgcatca cggtggccgt ccgccgcgag cggatgtacg acggtcgcgg ccagaacatc
     6361 ctggacgccc tcgacctgga ccgctacacg ctgctcccca acacggccgg ctgctacagc
     6421 gccgccgacg ccgtgcgtgt ggcccggatg ggccgcgaaa tcctgctgag cctggagaac
     6481 cccggggccg attgggtcaa gctcgaatgc ctgggggacg ccaagacgct gctgcccgac
     6541 ccggtcgaaa cgctgcgggc gaccgagatg ctcgtggccg acggctttgc cgtcctctgc
     6601 tataccagcg acgatccagt aatcgcccgc cggctgaaaa aggcgggggc ggccgccgtc
     6661 atgcccgccg gcagccccat cggctccggc cagggggtgc tcaaccccaa caacatccgg
     6721 atcatcctgg agtacctcaa ggccgaagat cccagctatc cggtgatcat cgacgcgggc
     6781 gtcggtacgg ccagcgacgt gaccgcggcg atggagctcg gcgtcgatgc ggtgctgctg
     6841 aacaccggca tcgcccacgc ccgcgacccg ctgggcatgg ccagtgccat gaaggccgcc
     6901 tgcgaagccg gccggctggc gtggctctcg ggccgcattc ccaagcggct ctacgcctcg
     6961 gccagcagtc ccgaagaggg cgtaatctcg atgcgtccct atcccgcgcc atgagctggc
     7021 gcaacgttgc ctcagcgtaa aaccgccgcg gttgttacgt gtatgacatc cgcgccgcgg
     7081 cgcgttttcg cagaaaaatc gcggggcagc ggttcttcct gattaaccgc ccggacgttt
     7141 gccgttctag tttgagacgg actgtcgcag aaaagaatcg gggtggattc ggattagccg
     7201 gtgagggtag aatccgccgc ggatggccaa tcacgcaaac actcagacag gaacgagtta
     7261 gagcattttg atcggtgtgc tgtcacggag ggcacggcag ttgcatcagt gatggtgcga
     7321 cgatcggaac tgcccttctg atcgttacgg caggagacgc cgccatgcta aagcccatcg
     7381 aaaaacccgc cggccgccag ccgctcgtgc accatccgtc cgatgtgctg gactgcttct
     7441 gccgatacga agtgaccgac cgcacctggc tcgactttga ccaggaaatc agccagcagc
     7501 tcctggaact ggaattcgcc aaccgccagt tcatccgcgt caagccgcag tcttcgcggc
     7561 gatagcttac ggggcggggg cgtcgaagat cgtccacacg acggtgttct tttgtagctc
     7621 ggccaggaac ttccgctggt cctcgcgctt cttctgcagg acgatcgcct ccctgatctt
     7681 cggctgcacg tcaaagaacg acagcgtggc ggcatcctgt cgttccttga cccgcacaat
     7741 gtggcagcca gtgtcgtcgt cgatgggccg gctcagccgc tcgagtggga gcgagaagac
     7801 ggcggcgtcg atcgcctggc tggcgaggct gcccttctgg acccagtcgt aatagccgcc
     7861 ctgtttggcg ttgggctcct gcgaaagctc ccgggcgacg gcggacagcg gcttgccata
     7921 gatcacctgg ttgcccatca tcgccagggc gtcgcgggcg gcatccttgt ccgggaagtt
     7981 cgcccacttg accgtcagga tttcgaagcg ggccttggcc ggcacggcaa agtcggccag
     8041 gtgctgctcg tagtagtcga gcatctcctg gtgggtcacg gtcggcttct ggtcgatgtt
     8101 cctgttcacc gtctcgcggc ctaaggtccc ctcgctgaac tgcacgatct gctgctcgag
     8161 cgaagtgccc atcgtgcgga gcttggcgtc gagctcgctc atcgattcca gctggtggtt
     8221 gtgcatcagt gccgctagcg ggcccgcgac cggcacctgg ttcatcaggg ccgtgaggtc
     8281 ctccggcttg gccgtcggga cgacaccgcg gcaggtctcc agctgcttct cgaagaacga
     8341 ccgcagcttg ccatcgatcc ccgcgcgggc ttcggcgtac ttgtccttgg cgttcttgcg
     8401 gatttgccgg tcgaactcca ggtagagcat cttcgacgtc accagctgtt ccaacaccgg
     8461 cttggcgtac accgtgcggc cctgccgcag aaactcgcgc tccagcgcgg ccagcttctc
     8521 ctgcgtttcc gctgcatcga gccgctgctc ggccgccttc ttcagcagct cctgcttaat
     8581 tcgcgacagc tgcggg
//
LOCUS       DASRJB010000369         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3484629, whole genome shotgun sequence.
ACCESSION   DASRJB010000369 DASRJB010000000
VERSION     DASRJB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484629"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1376)
                     /locus_tag="VFB80_09730"
     CDS             complement(39..1376)
                     /locus_tag="VFB80_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34089.1"
                     /translation="MRLLSILAAGLCLGVSTSWAADHWPQWRGPSGDGVAEAKHPEQW
                     SADEHIAWKVEVPGVGWSQPIVWDGKVFLTTCLADQRRPPRPGDWTPGNLSAIFGSYK
                     RPPNIECEWKVLCLDAATGKTIWEQTPHTGKPRISIHANNSYATETCATDGERLIAYF
                     GMTGIYCYDLAGQLQWTKDLGSYPTQMDWGTGSSPVMHEELVFIQCDNNKDSFLVALD
                     KKTGDEFWRVERDELSNWATPIIWKNSQRTELVAGGGTKMRSYDPASGKLLWEMAASG
                     RCSPSPVAAGDLLFVGSADRLTGQRGIVAAIKPGASGDISLEGDSTTNEFVLWSSKLS
                     GHRVASPVVSADCLYLLEQQAGIVRCLDAKTGNEHYRKRLSGATGLTASPWTSGGKVF
                     CLDQSGQTFVLAAGPQFHVLHVNKLADEMFWASAAVAGESLFMRGVDHLYCVK"
     gene            complement(1450..2073)
                     /locus_tag="VFB80_09735"
     CDS             complement(1450..2073)
                     /locus_tag="VFB80_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34090.1"
                     /translation="MSTPSNAGPARPRGLQFTLAALLAAMTIAALFCVALARPTRFWS
                     ELVVLLVVVALLTSIPAIVYRSGRARAFAIGFLVFTLGFLLALVSRELLFRTPRYGYP
                     NDAFLSSHLGTWLFAKIHPNNDQPTDGISGLGMSVGFDSGMGSTVTGMSGGGNVMLGG
                     LNTAMPPPPKYDPARFGEIVHAGCALLAGIAGGLLTQFLHATRKREP"
     gene            2409..3398
                     /locus_tag="VFB80_09740"
     CDS             2409..3398
                     /locus_tag="VFB80_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34091.1"
                     /translation="MNARAAKSPAALAGRRFRLPLRFSLRTFLAAITILCVVLGWRVN
                     RASRQRDAVAAIRAAGGAVHYDYQQPSFGPEHSLGETDFAAQPSEPDWLLALLGIDFF
                     HHVTAVNLGSERPDRPVPANIASHLARFPRLRTLWLSGEFIGDAELAIVGRLGRLEIL
                     ACTSAAISDDGVAHLGNLPCLRHVELYYPQPGDRSLATLARLPNLEVIRLPSGRLTSA
                     GLQVLVGHRRLRKLNLGGRDKTSALDDDALAHLAQVPQLEELELAHTRISPAGLERLQ
                     TLASLRYLDLRGSSADDYATVAPLFPNCRVVALKRPPRPWLHLPFGAGDAVGD"
     gene            complement(3629..>4082)
                     /locus_tag="VFB80_09745"
     CDS             complement(3629..>4082)
                     /locus_tag="VFB80_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34092.1"
                     /translation="LRAIAAAKPAAGPEAAPYMDGHEVPPVFDVHLPKSIAAGSSLAG
                     NEFSVAYLPVDRELEANAQRWAKSDNPDFRWLAGRALIYFPSDRNAALLRTLLDDPAT
                     WSRREMFQLMQPLYPEDNPEYLVRWEAWHVLAGWGHKVPKPDFGKTPR"
BASE COUNT          714 a         1325 c         1306 g          737 t
ORIGIN      
        1 cgacttacca gtcatgaagt gacggtcgcc tagggacatc acttgacgca atacagatga
       61 tcgacgccgc gcatgaacag cgattcaccc gccactgccg ctgacgccca gaacatctcg
      121 tcggccagtt tattaacgtg gagcacatgg aactgtggcc cggctgccag gacgaacgtc
      181 tgcccgctct gatcgaggca aaaaactttg ccgccgctgg tccaaggcga agcggtgagg
      241 cctgtcgcac cgcttaggcg cttgcgatag tgctcattcc cggttttggc atccaggcag
      301 cgaacgattc cggcctgctg ctcgagcaga tacaagcaat cggccgacac cacgggcgac
      361 gcaacacggt ggccgctcag cttggaactc caaaggacga attcgttcgt cgtgctgtcc
      421 ccctcgagtg agatgtcgcc gctggcgccc ggctttatgg cggcgacgat gccccgctga
      481 ccggtcaggc gatcagctga gcccacgaag agcaaatccc ccgctgcaac cggcgacggc
      541 gagcagcgac cgctcgccgc catttcccac aacagcttcc cgctcgccgg atcgtacgat
      601 cgcatcttcg tcccaccgcc ggcgaccaac tccgtccgct gtgagttctt ccaaatgatc
      661 ggcgttgccc agttggatag ttcatcgcgc tcgactcgcc aaaactcgtc gcccgtcttc
      721 ttgtcgaggg cgaccaggaa tgaatccttg ttgttatcgc actgaatgaa aaccagctct
      781 tcgtgcatca cgggcgagct gccggtgccc caatccatct gcgtggggta gctgccgagg
      841 tccttggtcc actgcagctg gccggccaaa tcatagcaat agatgcccgt catgccgaaa
      901 tacgcgatca gccgctcgcc atccgtggcg cacgtctcgg tcgcatagct gttgttcgca
      961 tgaatcgaga ttcgcggctt gcccgtatgg ggcgtctgct cccagatcgt ttttcccgtc
     1021 gccgcatcca ggcacagcac cttccactcg cattcgatat tcggcggcct cttgtagctg
     1081 ccgaaaatgg cgctcaggtt gcccggcgtc caatcgcccg gccgcggtgg ccttcgctgg
     1141 tcggcgaggc aagtcgtcag gaagaccttg ccgtcccaga caatcggctg cgaccagcca
     1201 acgcccggaa cttccacctt ccaggcgata tgctcatcag ccgaccactg ctccggatgc
     1261 ttcgcctccg ccaccccatc ccccgacggc ccccgccact gcggccaatg atctgctgcc
     1321 cacgaggtcg aaacgcccaa gcaaagcccc gcggccagaa tagacaagag gcgcatgaaa
     1381 cgacctttca aatgggtcag gagtcggggg tcaggagtct gggctttcgc cgaggtgagc
     1441 tcgtcggcac taaggctcgc gctttcgcgt tgcgtggagg aactgcgtca gcaggccgcc
     1501 ggcgatgccg gctaggaggg cgcagccggc gtggacgatc tcgccgaagc gggcggggtc
     1561 gtatttgggc ggcggcggca tcgcggtgtt gagaccaccc agcatcacgt tgccgccgcc
     1621 tgacatcccg gtgacggtgc tgcccattcc gctgtcgaag ccgacggaca ttcccaggcc
     1681 cgaaattccg tcagtcggct gatcgttgtt gggatggatt tttgcgaaga gccacgtgcc
     1741 caggtggctg ctcaggaagg cgtcgttggg gtaaccatac cgcggggtgc gaaacagcag
     1801 ctcgcggctg accagggcca gcagaaagcc gagcgtgaat accaggaagc cgatggcaaa
     1861 ggcccgcgca cgcccgctgc gatagacgat tgccggaatg ctcgtcagca gggcgactac
     1921 cacgagcagc accaccagct ccgaccagaa ccgcgtcggc ctggccagcg ccacgcagaa
     1981 gagcgcggcg atggtcatcg cggccaagag tgccgccagc gtgaattgca gaccacgggg
     2041 ccgcgcgggc ccggcgtttg acggcgttga catacggtgg cactcccctt caggtgcggc
     2101 cggtcggcgg ccgctgcaca tccaggatac ctgagctgag gagcaccgtt cagccacggg
     2161 ccggctgccg cggcgcacct ctggtcccct ctccctttgg gagacggtaa gggagcgggt
     2221 tttggcttgc cgtgagctga tcgtcgtggt cctcggcagc accaccggcg acgcccgcta
     2281 cacgggcagc cgcaacctct tccgttgggc ctggtcgcag ccctaaccgc tgccggcgtc
     2341 gtcaagcgct gcgtaccaga gcgagtattg cgattgcgac catccacccc tcgccgcaca
     2401 atgggaccat gaacgcccgc gctgcgaaat cgccggcagc attggccggt cggcgcttcc
     2461 ggctgccgct gcgatttagc ctgcggactt tcctcgccgc gattacgatc ctctgcgtcg
     2521 tcctcggctg gcgggtcaac cgggcgagtc ggcagcgcga cgccgtggcg gcgattcgcg
     2581 cggcgggcgg ggccgtgcac tacgattatc agcagccctc gtttggacct gagcacagct
     2641 tgggtgaaac cgattttgcc gctcagccca gcgagccaga ctggctgctc gctctcttgg
     2701 gcatcgactt ctttcaccac gtaaccgccg tcaatctcgg ttccgagcgg cctgacaggc
     2761 cggttccggc gaacatcgcc tcgcacctgg cccgcttccc gcgcctccga acgctctggc
     2821 tgagcggtga gtttattggc gatgccgagc tcgcgatcgt tggccggttg ggccggctcg
     2881 aaatcctcgc ttgcacgagc gccgcgatca gcgacgacgg cgtcgcccat ttgggcaacc
     2941 tgccctgcct gcggcatgtc gagctctact atccgcagcc gggggaccgc agcctggcaa
     3001 ctctcgcgag gttgccgaac ctcgaagtca ttcgcctgcc ttccggccga ttgaccagcg
     3061 ccggcctgca ggtgctcgtc ggtcatcgcc ggctgagaaa gttgaatctc gggggccggg
     3121 acaaaacgag cgcgcttgac gacgacgcgt tggcgcatct ggcccaggtg ccacagctcg
     3181 aagagctcga actggcccac acccgaatct cgccggccgg tttagagcgg ctgcagacgc
     3241 tggcgagctt gcgctacttg gatctacggg ggagttccgc cgacgattac gccaccgttg
     3301 ctccgctctt tccgaattgc cgggtcgtgg ccttgaaaag gcctccgcgg ccctggctgc
     3361 acctgccctt cggggccggt gatgccgtag gcgattgact atccgccggg attttgggag
     3421 cggagcgggc gtagtccgaa agtccgaaag ctgcgacgag tcgcagcact ccaaagtagg
     3481 tcccggctgg cggacggggt ctgaagcgcc gccgtgttct cgctgtagcc ggcttcggcc
     3541 ggcttcagcc ggggctatgt gggaggaccc ggaaatcctg cagccggctt aagcaggctg
     3601 caatttgact gaggtgcgca cagcggcatc agcgcggggt ttttccgaag tctggcttgg
     3661 gtactttgtg gccccagccg gcaaggacgt gccaggcttc ccagcggacg aggtattcgg
     3721 ggttgtcttc gggatagagc ggctgcatga gctgaaacat ttcgcggcgg gaccaggtgg
     3781 cggggtcatc aaggagcgtg cgaaggagcg cggcgtttcg gtcggagggg aaatagatca
     3841 gggcacgacc ggcgagccag cggaagtccg ggttgtcgga cttcgcccac cgctgggcgt
     3901 tggcttccag ctctcgatcg acgggcaagt aagcgacaga aaattcgttg ccggccaggg
     3961 atgagccggc tgcgatcgat ttgggaagat gcacgtcaaa aaccggcggg acctcgtggc
     4021 cgtccatgta cggcgcggct tctggaccag cggcgggttt cgcggcggca atcgcccgaa
     4081 gc
//
LOCUS       DASRJB010000370         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_386440, whole genome shotgun sequence.
ACCESSION   DASRJB010000370 DASRJB010000000
VERSION     DASRJB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386440"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFB80_09750"
     CDS             <1..861
                     /locus_tag="VFB80_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN34093.1"
                     /translation="AAPSHATGRQPLVPLAAVADVRIVEGPAMIKSENGRLLNYVTLM
                     VRGRDMVGFVEEARRAVAQKVKLPEGVSIAWTGEFEHQVRAARTLRLVFPAVVVLIFL
                     ILYLTYHDLADAALMMLAVPEALAGGAFFMYLFPKIMQGWDAPPLDFSVAVWVGFIAC
                     FGMATETGIIMLVYLREAIDKRGGLENIRSLEELQQAVIEGAVHRLRPKLLTEGVAII
                     AIFPMVFASGVGGEILAPMALPVLGGLLISDEVVDLFLPVRFYWVRRARWLKLQEQRR
                     NDSARPLQPA"
     gene            954..1424
                     /locus_tag="VFB80_09755"
     CDS             954..1424
                     /locus_tag="VFB80_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34094.1"
                     /translation="MYSFDERKKSHTVQSRVRRLLDLTVPNFADQLQSERLEERYNRT
                     LPALLCAWENDQPVTDSCVYVVTKDVASEGVGILLQQPFRADKVMLAFWLDHTEAREP
                     WFFVGVGRNLRKIGAGFFTMGVQLTDFVGSGCYGNRAPLAPLMKQLLPPAATPV"
     gene            complement(1427..2701)
                     /locus_tag="VFB80_09760"
     CDS             complement(1427..2701)
                     /locus_tag="VFB80_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN34095.1"
                     /translation="MQNSAARSYWPVVILLMLVVGLGHFNRVGMSVAGTERIIPEYKI
                     APDQMGLVYSAFLLFYTLAMLPGGWFIDRFGARAALAAYGFASAAFVALTGCVGLLWD
                     DARGVWLGLIAVRSLMGVVNAPLHPASARMVYGHVPAGAKSLANGLVTFAACVGIAGT
                     FYILGPLIDRWDWPRAFLITSVFTAAVACVWLVGTRYATAAPAGGGKLEMAAPRLSEL
                     WPLLGRRSVICITLSYAAMGFFQYLFFYWIEYYFETVQKQGVDVARQYSALTTLAMGA
                     GMVCGGWLADKVPAAFGPRIRRRLVPMLGMIASGVVFELGLLSPDIRVTLAAFALAAA
                     LLGATEGAFWTTVVELGGRFGGTAAGLMNTGGNAGGTLSPYVTPLLSTYFAAQFGEEL
                     GWRLGLSVAGAVSILGALLWCGVDPGRDAAEA"
     gene            complement(2714..>2931)
                     /locus_tag="VFB80_09765"
     CDS             complement(2714..>2931)
                     /locus_tag="VFB80_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN34096.1"
                     /translation="PITAAASLHWLSTCRDAFLFEDCVDDSPLRHELTHERVQAEGGW
                     IRVPDGPGLGVTLNEEFVRAHLVAESL"
BASE COUNT          530 a          993 c          951 g          457 t
ORIGIN      
        1 gccgctccgt cgcacgccac tggccggcag ccgctggtgc ccctggcggc ggtggccgac
       61 gtgcgaatcg tggaaggccc ggcgatgatc aaaagcgaaa acggccggct gctgaactac
      121 gtcacgctga tggtccgggg ccgcgacatg gtcggctttg tcgaggaggc ccggcgggcc
      181 gtggcccaga aagtgaagct gcccgagggc gtgagcatcg cctggaccgg cgagttcgag
      241 caccaggtgc gggccgcccg cacgctgcgg ctggtcttcc ccgcggtcgt cgtgctgatc
      301 tttctcattc tgtatctgac gtatcacgac ctggccgacg cggccctgat gatgctggcc
      361 gttcccgagg ccctggccgg cggcgcgttc tttatgtacc tctttcccaa gatcatgcag
      421 ggctgggacg cgccgccgct cgatttcagc gtggcggtgt gggttggctt catcgcctgc
      481 ttcggcatgg cgaccgaaac gggcatcatc atgctcgtct acttgcgcga ggcgatcgac
      541 aagcggggcg gcctggaaaa catccgctcg ctcgaagagc tgcagcaagc cgtcattgaa
      601 ggcgcggttc accggctgcg gcccaagctg ctcaccgagg gcgtggcgat catcgccatc
      661 ttcccgatgg tctttgcctc cggcgtcggc ggcgagattc tggcgccgat ggctctgccc
      721 gtgctcggcg gcctcttgat ctccgacgaa gtcgtcgatc tgttcctgcc tgtccgcttc
      781 tactgggtcc gccgggcgcg gtggctcaag ctccaggagc agcggcggaa tgattccgca
      841 aggccgctcc agccggcata accggacccc ggtgggatga ttctttggac ggcctccgcc
      901 ggaaaaagct acgtttgctg acaaacggca ccatctcgca caaggcagcc gccatgtatt
      961 cgttcgatga acgcaagaag tcgcataccg ttcagagccg cgttcgccgg ctgctggacc
     1021 tgacggtgcc caacttcgcg gaccagctgc agtccgaacg gctcgaagag cgctacaacc
     1081 gcacgctgcc ggcattgctc tgcgcctggg aaaacgacca gccggtgacg gacagctgcg
     1141 tgtacgtcgt gaccaaggac gtcgccagcg aaggcgtggg aatcctgctc cagcagccgt
     1201 tccgggcgga caaggtgatg ctggccttct ggctcgatca caccgaagcc agggagccct
     1261 ggttcttcgt gggcgtcggg cggaacctgc ggaagatcgg ggcgggattc ttcacgatgg
     1321 gcgtccagct caccgacttc gtcggctccg gctgctatgg caaccgtgcg ccgctcgcgc
     1381 cgctgatgaa gcagctgctt ccgccggcgg cgacaccggt ctgaattcac gcctcggcgg
     1441 catcgcgccc cggatcgacg ccgcaccaga gcaaagcgcc gaggatcgaa accgcgccgg
     1501 ccacggagag ccccagccgc cagccgagct cctcgccgaa ctgtgcggcg aaataggtgc
     1561 tcaagagcgg cgtgacgtag ggcgagagtg tcccgcccgc gttgccgccg gtgttcatca
     1621 ggcccgcggc cgtgccgcca aagcgtccgc ccaattcgac gaccgtcgtc cagaaggcgc
     1681 cttcggtggc tcccagcagg gccgcggcca gggcgaacgc cgccagcgtg acccgtatat
     1741 cagggctgag caggcccagc tcgaaaacca cgccgctggc gatcatgcct agcatcggca
     1801 ccagccggcg gcggatgcgc gggccaaacg cggccggaac tttgtcagcc agccagccgc
     1861 cgcagaccat ccccgccccc atcgccagcg tggtcagggc cgaatattgc cgtgcgacat
     1921 cgacgccctg cttctgcacc gtctcgaagt agtattcgat ccagtagaaa aacaggtatt
     1981 gaaagaatcc catggcggcg tagctgagcg taatgcagat gacgctgcgg cgacccagca
     2041 agggccacaa ctccgataag cgcggcgctg ccatctcgag tttgcctccg ccggccggcg
     2101 cggcggtcgc gtagcgcgtg cccacgagcc agacgcaagc caccgccgcc gtgaaaacgc
     2161 tcgtgataag aaaggcccgc ggccaatccc agcgatcgat gagcggcccc aggatataaa
     2221 aggtgccggc gatcccgacg caggcggcga aggtcaccag gccgttggcg agcgatttcg
     2281 ctccggccgg cacgtggcca tagaccattc gggccgacgc cggatgcagc ggcgcgttga
     2341 cgacgcccat cagcgagcgc acggcgatca gtcccagcca gacgccccgc gcatcgtccc
     2401 agagcaggcc cacgcagccg gtcagcgcga caaacgcggc cgaagcgaag ccgtacgccg
     2461 ccagcgccgc gcgagcaccg aatcggtcga tgaaccagcc gccgggcagc atcgccagcg
     2521 tgtaaaagag caggaacgct gaatagacca ggcccatctg gtccggcgcg atcttgtact
     2581 ccgggatgat ccgctcggtc cccgcgaccg acatgccgac gcggttgaag tgccccaggc
     2641 cgacgacgag catcagcagg atcacgaccg gccaataact tcgggctgct gaattctgca
     2701 ttctgaagaa ttcctaaagc gactccgcga cgaggtgtgc ccgaacgaat tcctcgttga
     2761 gcgtcacgcc caaaccggga ccgtcgggca cgcgaatcca gccgccttcg gcttgcaccc
     2821 gctcgtgcgt cagctcgtgc cgcagcggcg aatcatcgac gcagtcctcg aacagaaacg
     2881 cgtcgcggca ggtgctgagc cagtgcaggc tcgccgccgc cgtgatcggg c
//
LOCUS       DASRJB010000371         9145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4052224, whole genome shotgun sequence.
ACCESSION   DASRJB010000371 DASRJB010000000
VERSION     DASRJB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4052224"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="VFB80_09770"
     CDS             complement(<1..1934)
                     /locus_tag="VFB80_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZN34097.1"
                     /translation="MRLQAARAAIALGLILLAAIPAAAQRRGFGGGAAGVYKSRIEPH
                     WFDGGARFWYQNDLPRGMREFNLVDAEKGTRSPAFDHERLATALKEAEVADVRADRLP
                     LEAIEFKTADNAVIFRAGSKDWKCDLGTYALTEVKDRPAPPAAPRGGGFRGGPAGRGG
                     GGLRGNPGGGIAGGFRGRGGPVRSPDGKWTASIVNSNIVLRDADGKESPLTTDGREGL
                     AYGRLEWSPDSQTLVAFKIEPGEEKQVHLVETSPENGGRAVLHSRNYALPGDKFTAYE
                     LCLFNPAEQKPIECKVDRMDFGSPRLRWAADGRTFSYQQVDRGHQRLRLIEIDSHTGN
                     SRNLIDEQTKTFIWTAHTENVGLSPFTWLRGGEEIIYCTEKDGWRHLYLVDAKSGSIK
                     NLITEGDYVVRGVDRIDEEKRQIWFRASGRNSDQDPYFIHYYRVNFDGTGLTALTAGN
                     GTHSVQYSPDDKYLIDTYTRVDQPAIHELRRVDNGELICKLDEADIGELKGRGFEPPE
                     VFVTKGRDGQTDIWGIICRPRNFDPAKKYPVIEKIYAGPQGSFTPKSFSTQGLNSTLT
                     DLGFAVVQMDGMGTANRSKAFHDVCWHNLGDAGLPDHILWHKAVAAKYPWYDISRVGI
                     YGGSAGGQNSTGAMLFQPDF"
     gene            complement(2023..2838)
                     /locus_tag="VFB80_09775"
     CDS             complement(2023..2838)
                     /locus_tag="VFB80_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34098.1"
                     /translation="MQTAGFWRSKCVIVTGASSGIGRAVALDLAGRGAKLGLIARRED
                     ALAAVASEIRAAGARAEYQAVDVAAAEPLAAAVRMLEESLGPCDIALANAGIYRKTEA
                     AAFAAADASAVIATNVLGVSNLFAAVLPGMLARKSGRLAAVASIAGLLGLPGGGAYSA
                     SKAAVITLLQSLRLDLAPRGIAVTVILPGYVDTPMITNRERRTLPGLVTAEDAARRIA
                     RAIERGQGVAAFPWQTWLSARLAGLLPPGLFQFVMGSRPPLEEAPPASESPAD"
     gene            3285..4550
                     /locus_tag="VFB80_09780"
     CDS             3285..4550
                     /locus_tag="VFB80_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34099.1"
                     /translation="MDRPFIHAAAGLAAALAAFAAAPSLRGEVFVLKSGGRIEGEHLN
                     PQRTAGQPYQIQTDGGLKLALAESAVARVVVASDVEREYEARRSGVPNTVNGHWDMAE
                     WCKEAGLAGERRRHLLAVIALDSDHAEARRVLGYSRYGSRWLTQNEFLQSQGYVPYKG
                     TWRLQQEIEIDIRNREREAAVKKYGQAIRNAFEQIGEGSRHAANSERLLNELTDPNAA
                     PALSEILRDSRQPRSVRLRCLEILGRLPPGLATATLVKVAMDDQDDTVRDRCLDELIR
                     GGPHVAGPGFLSELKVDKSSKNYKNEQNWRVNRAAYCLGRLGYTDATLPLIEALTTEH
                     QEVVQQSSSPTGGTPLSFGSGGPNGGMGSFGVGGRPKVRVTKRDNAGVRDALTTLHPG
                     VNHQYDADAWMRWYIQKFTTTKVDLRRDE"
     gene            complement(4552..5730)
                     /locus_tag="VFB80_09785"
     CDS             complement(4552..5730)
                     /locus_tag="VFB80_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZN34100.1"
                     /translation="MTYIAPELHPPVRVLMGPGPSDIHPRVLQALAKGTVGHFDPAYL
                     EIMNGLQEMLRGLFRTKNQMTMTISGTGSAGMETTVVNLIEPGDSMVVCVNGMFGSRM
                     ADVAERAGAKVTKVERPWGEVFTPDHLKAALATGPKVVGIVMAETSTGARQSIPEISQ
                     AVHDAGAMLLVDTVTALGGIPVEVDAWKIDAIYSATQKCLSCPPGLSPVSFSSRAMEV
                     ITSRKKKVQSWYLDVSLLANYWGQNRVYHHTGPINMTYALYEALRLIHIEGLENCHAR
                     HLLNHRALKAGLASIGIQYATAESCTLPQLNAVLVPAGVDDAQIRSGLLTRFGIEIGA
                     GLGAYKGKVWRIGLMGYGSRPANVLLFLSALEQLLAEQGYNFQRGASIAAANGVYLAK
                     "
     gene            5970..7097
                     /locus_tag="VFB80_09790"
     CDS             5970..7097
                     /locus_tag="VFB80_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34101.1"
                     /translation="MPHTHTPTMQSRGLLAVFALLAVLGSALAWSKGQIPRREMQPAA
                     AAPDLQLYREVVAEVRGGKSYYDAARVKIPQFGFPISSPLNWRLPTYAWLFALLPLPR
                     YVQALLVLLSLAALLLAYRAQTKNGPALVAAFTLLALLGVVRWSLDGDAYLAQEPWAA
                     TLIAISLATHSLGGRWRSASVAAGTTALLFRELALPYCGIACLSAAWNRRWRECAAWA
                     AGIALFFAFFAWHVSQVRGQLAGMEAAAPGAGLAQWLRLGGLDFVLLTTRMNGLLFAA
                     PAALVWLYLLASLVGLAHRGDETSRLACLAALAYLLAFAILGRPENFYWGLLPAPLLA
                     WGTASAPAALARLWQAAQADIPAVPFVTPSPAGAGQPPAET"
     gene            7134..7937
                     /locus_tag="VFB80_09795"
     CDS             7134..7937
                     /locus_tag="VFB80_09795"
                     /inference="COORDINATES: protein motif:HMM:NF042691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6585 family protein"
                     /protein_id="HZN34102.1"
                     /translation="MSQISFLSSLSALPSADQVREYPGSDVPPEIQALGSPREVHRPG
                     SFAALANFRYPLAALVVAAFGGLAYILYQDLASKNQLDGNALFVFAVIGALAVSFAAL
                     ILRIPVTNSTVFFYSRAAVVAQKDKLTLIPWKQLLYRRSTISTPEGRQFYCGWLESFD
                     TFEERIWEYSAEHWLPEAEAKIKAGETVKVGELAISAKDISYQGRTTTWDRVTRLLLL
                     VGRVYQLHISTKDTWCQWATIDMHQIPNARAVQQLITNICPPHLLTTAD"
     gene            8266..>9145
                     /locus_tag="VFB80_09800"
     CDS             8266..>9145
                     /locus_tag="VFB80_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZN34103.1"
                     /translation="MPKHIRSATRRTFLQAAAAVAAIPLAPTILGAQDKAGTKRPVIG
                     SGEHTYECVHNWGELPSHVKWGETHGVAVDAAGLIYIKHRNNAADPMDAIVVFDPAGK
                     FVRSFGDEYNGGGHGIDIRKEGNTEFLYLCDVKHNLVTKTTLKGEFVWTKGRPPEPGV
                     YKDPAVKYSPTNVAFGPDGGFYIGDGYGSHYIHQYDKDANWVRTWGGGGTEPGKMRTP
                     HGLWLDNRPGREPTLVVADRANARLQYFTLDGQHLSFVNEVSFPAHFDIRGDVLLVPD
                     LHARVSLFGKDNKVIAH"
BASE COUNT         1523 a         3049 c         3006 g         1567 t
ORIGIN      
        1 tagaaatcgg gctgaaacag catcgccccg gtcgaattct gcccgcccgc cgagccgccg
       61 tagatgccga cgcggctgat gtcgtaccac ggatatttgg cggcgactgc cttgtgccag
      121 aggatgtggt cgggcaggcc ggcgtcgccc aggttgtgcc agcagacgtc gtgaaacgcc
      181 ttgctgcggt tggcggtgcc catgccgtcc atctgcacga cggcaaagcc caggtcggtc
      241 agggtggagt tcaggccctg cgtggaaaag ctcttcggag tgaacgagcc ctgcgggcca
      301 gcataaatct tttcgatgac cgggtacttt ttcgccggat cgaaattccg cggccggcag
      361 atgatgcccc agatgtccgt ctggccgtcg cggcccttgg tgacaaagac ctctggcggc
      421 tcaaagccgc ggcccttgag ctcgccgatg tccgcctcgt ccagcttgca gatcagctcg
      481 ccgttatcca cccgccgcag ctcgtgaatc gccggctgat cgacgcgggt gtaggtgtcg
      541 atcaggtatt tgtcgtcggg cgaatactgc acgctgtgcg tcccgttgcc tgcggtgagc
      601 gccgtcaggc cagtgccgtc gaaattcaca cggtagtaat ggatgaagta cgggtcctga
      661 tcggagttgc gtccgctcgc gcggaaccag atttgccgct tctcttcgtc gatccgatcg
      721 acgccgcgaa cgacatagtc cccctcggtg atcaggttct tgatcgaccc gctcttggca
      781 tcgaccaggt agaggtgccg ccagccgtcc ttctcggtgc aatagatgat ctcttcgccg
      841 ccgcgcagcc aggtaaacgg cgacaggccg acgttctcgg tgtgggcggt ccagatgaag
      901 gtcttggtct gctcgtcgat caaattgcgg ctgttgcccg tgtgcgagtc gatctcgatc
      961 agccgcaggc gctggtggcc gcgatcgacc tgctgatagc tgaaggtgcg gccgtcggcg
     1021 gcccagcgga ggcggggcga gccgaaatcc atgcgatcaa ccttgcactc gattggtttt
     1081 tgctccgcgg ggttgaacag gcacaattcg taggccgtga acttgtcgcc aggcagcgca
     1141 taattgcggg agtgcaggac tgcgcggccg ccgttttcgg gcgaggtctc gaccaggtgg
     1201 acctgcttct cttcgcccgg ctcgatcttg aacgccacga gcgtttgcga atcgggcgac
     1261 cattccaggc ggccgtaggc caacccctcg cggccgtcag tggtcagcgg cgattccttg
     1321 ccgtcggcgt cgcggaggac gatgttgcta ttgacgatcg acgccgtcca tttgccgtcg
     1381 ggcgagcgga cgggcccgcc gcgcccgcgg aagccgccgg caattccgcc gccgggattg
     1441 ccccgcaatc cgcctccgcc gcgccctgct ggacctccgc ggaatccacc gccgcggggt
     1501 gcggccggcg gcgcgggccg gtccttgacc tcggtgagcg cgtaggtccc cagatcgcac
     1561 ttccagtcct tgctgcccgc gcgaaagatc acggcgttgt cggccgtttt gaactcaatc
     1621 gcttccagcg gcagccgatc agcccgcacg tctgcgactt cggcctcttt cagggctgtc
     1681 gccagccgct cgtgatcgaa ggctggggag cgcgttccct tttcggcatc gaccaggttg
     1741 aactcgcgca tgccgcgggg caggtcgttc tggtaccaga accgcgcgcc gccgtcgaac
     1801 cagtggggct cgatccgcga cttgtagacg ccggcagcgc cgccgccgaa tcctcgccgc
     1861 tgcgccgccg cgggaattgc cgccagaaga atcaatccga gggcgatcgc cgcccgagcc
     1921 gcctgcaagc gcatggggaa cctccgcgga aaagacgtgc aatcagaacc cccagcgacg
     1981 ccttgatggt tctcgccgcg agagccgttg tcaagccagc ggtcagtcgg cggggctttc
     2041 gctcgccggc ggcgcttcct ccagcggcgg cctgctcccc atcacgaact gaaaaaggcc
     2101 gggcggcaag agaccggcca ggcgggcgct cagccacgtc tgccagggaa acgccgcgac
     2161 cccctgcccg cgctcgatcg cccgcgcgat ccgccgggcg gcatcctcag ctgtcaccag
     2221 tccgggcagt gtccgccgct cccggttggt gatcattggc gtgtcgacgt atcccggcag
     2281 aattaccgtc acggcaatcc cgcgcggggc caggtcgaga cggaggcttt gcagcagcgt
     2341 gatgacggcc gccttgctgg ccgaataggc cccgcccccc ggcaggccga gcagccccgc
     2401 gatgctggct acggccgcca atcggccgct cttgcgggcc agcattcccg gcagcaccgc
     2461 cgcgaacagg ttgctcaccc ccagcacatt ggtcgcgatg accgcgctgg cgtcggcggc
     2521 ggcgaaggcc gcggcctcgg tcttccgata gatgcccgca ttggccagcg cgatgtcgca
     2581 cggccccagc gactcctcga gcatccgcac cgctgccgcc aacggctcgg ctgcggcgac
     2641 gtcaaccgcc tgatactccg cccgcgcacc tgccgctcga atttcggacg cgacagcagc
     2701 caatgcatcc tcgcgccgcg cgatcagccc cagcttcgcg ccgcggcctg ccagatcgag
     2761 tgctacggcc cggccaatgc cgctcgacgc gccggtgacg atgacgcact tgctacgcca
     2821 gaagccagcc gtttgcatcg ctccagccta atcgactgcg gccgccgcgt gctccgctta
     2881 gccccggccc ccaggccggc tcggcggaac aacgtcaccc ccaactcagc ggcgtggacg
     2941 ccggggctaa gcgtcgggca agcgtggggc gaattgagcg acatcggcca tttgtgaaca
     3001 ttggtactct atttgcagta tagttcccat tgcggggcgg gctgccattt tggcctgacg
     3061 ccgtggcggg aactcatata acgaaaaccc gcgggcgaca cttctaaccc ttataaacct
     3121 aatgcgaaac cgagggcagg atttcgccac aaccagtgcc gcaccaaaga tttgcgtcgt
     3181 gacctccggc gcgaacagac atcaccgacg ctcgctaatg cccaagtctt ccttatatac
     3241 tgggagtcgc ggcgagcagg ggcgctgcag tgttggctag aaacatggac cggccgttca
     3301 ttcatgcagc cgcaggactg gcagctgcgc tcgccgcctt cgcagccgcg ccgagcctgc
     3361 gcggcgaggt cttcgtcctc aagtcgggcg ggcggatcga aggggagcac ctcaatccgc
     3421 agcgcaccgc cgggcagccg tatcagattc agaccgatgg cgggcttaag ctcgccctgg
     3481 ccgaaagcgc ggtggcccgc gtcgtggtgg ccagcgacgt cgagcgggaa tacgaggccc
     3541 ggcggtcggg cgtgcccaac acggtgaatg gccattggga catggccgag tggtgcaagg
     3601 aggccggact cgcaggcgag cggcggcggc atctcttggc cgtcatcgca ctcgattccg
     3661 accatgccga ggcccgccgc gtgctcggct attctcgcta cggcagccgc tggctgacgc
     3721 agaacgaatt cctgcagagc cagggttacg tgccctacaa gggcacgtgg cgtttgcagc
     3781 aggagatcga gatcgacatc cgcaatcgcg agcgggaggc ggccgtcaag aaatatggcc
     3841 aggcgatccg caacgccttt gagcagatcg gtgaagggag ccgccacgcc gccaactccg
     3901 agcgcctgct gaacgagttg accgatccca acgccgcgcc cgcactgagc gagatcctgc
     3961 gcgattcgcg gcagccgcga agcgtgcggc tgcggtgcct ggagattctg ggccggctgc
     4021 cgccggggct ggccacggcg acgctggtca aggtggcgat ggacgaccag gacgacacgg
     4081 tccgcgaccg ctgcctggat gagctgatcc gcggcggccc gcacgtcgcg gggccggggt
     4141 tcttgagcga gctaaaggtc gacaagtcgt cgaaaaacta caagaacgag cagaactggc
     4201 gggtgaatcg cgcggcctat tgcctcggtc ggctcggcta caccgacgcg acgctgccgc
     4261 tgatcgaggc gctgacgacc gagcatcagg aggtcgtgca acagtccagc agcccaaccg
     4321 gtggcacgcc gctgagcttt ggctcgggag gacccaacgg cgggatgggc agcttcggcg
     4381 tcggcgggcg gccgaaggtg cgggtgacca agcgcgataa cgccggtgtt cgcgacgcgc
     4441 tgaccacgct gcatcccggc gtcaatcatc agtacgacgc cgatgcctgg atgcggtggt
     4501 acatccagaa gttcacgacg acgaaagtcg atttgcgccg cgacgagtga gctacttcgc
     4561 gagatacacg ccgttggccg cggcaatgct cgcgccgcgc tggaagttat agccctgctc
     4621 ggccagcaat tgctccaggg ccgacaggaa caacagcacg ttggccggcc gcgagccgta
     4681 gcccatcagg ccgatccgcc agactttgcc tttgtaagcg ccgaggcctg cgccgatctc
     4741 gatcccgaag cgggtgagca ggccgctgcg gatttgcgcg tcatcgacgc ccgcgggaac
     4801 gagcaccgcg ttgagctgcg gcagcgtgca ggactcggcc gtggcgtact gaatgccgat
     4861 cgaagccagg ccagccttga gggcccggtg attgaggagg tgcctcgcgt ggcagttttc
     4921 cagcccttcg atgtggatca gccgcagggc ctcgtacagc gcgtacgtca tgttgatcgg
     4981 gccggtgtgg tgatagacgc ggttctggcc ccagtagttg gccagcagcg agacgtcgag
     5041 gtaccagctc tggactttct tctttcgcga ggtgatgacc tccatcgccc gcgagctgaa
     5101 cgagacgggc gagaggcccg gcgggcagga gaggcacttt tgcgtagcgg aatagatggc
     5161 gtcgatcttc caggcgtcca cctcgacggg aatgccgccg agggcggtga ccgtatcgac
     5221 gagcagcatc gcgccggcgt cgtggacggc ctgcgagatt tccggaatcg actgccgcgc
     5281 gccggtcgag gtttcggcca tcacgatgcc gacgaccttc ggcccggtcg ccagcgccgc
     5341 cttgagatgg tcgggcgtga agacttcgcc ccagggccgc tcgaccttgg tgaccttcgc
     5401 gccggcccgc tcggccacgt cggccatccg cgagccgaac atgccgttga cgcagaccac
     5461 catcgaatcg cccggctcga tcaggttgac gactgtcgtc tccatccccg cggagccggt
     5521 cccgctgatg gtcatcgtca tctggttctt cgtgcggaac agtccgcgga gcatttcctg
     5581 cagcccgttc atgatctcca ggtaggccgg gtcgaagtgg cccaccgtgc ctttggccag
     5641 cgcctggagc acgcggggat gtatatcgct cggaccggga cccatgagaa cgcggacggg
     5701 cggatgcagc tcgggagcga tgtaagtcat ggagtcaggc gtcaggagtc gggggtcagg
     5761 agtcgggcgg cagccgcggg agtggctgcc gcgccgccca acaccagcag caacgccgcc
     5821 ggccagtagc cctggccgcg ttttcggcgc gtcatagccg gtatgacccg taaaggcgtt
     5881 tctctcctgc atcctacact tgcgcagaac tcgccacgag gcttcgtagg gcgggctctg
     5941 cccaccacaa ttctgaaaac gaggcggctg tgccgcacac ccacacgcca acgatgcaaa
     6001 gccgcgggct gctcgcggtg ttcgcactgc tcgccgtcct gggcagcgcc ctggcctgga
     6061 gcaagggcca gattccccgc cgcgagatgc agccggccgc cgccgcgccc gacttgcagc
     6121 tctatcgcga ggtggtcgcg gaggtccgcg gcgggaagag ctactacgac gccgcccgcg
     6181 tcaagattcc gcagttcggc tttcccatca gctcgccgct caactggcgg ctgccgacct
     6241 atgcctggct gttcgcgctg ttgccgcttc cgcgctacgt tcaggcgctg ctcgtgctcc
     6301 tgtcgctggc ggcgctcctc ctggcttacc gcgcacagac gaagaacggc ccggcgctgg
     6361 tcgccgcctt tacgctgctc gcgctgctcg gcgtggtccg ctggtcgctg gatggcgacg
     6421 cgtatctcgc gcaggagcct tgggcggcca cgctcatcgc gatttcgctc gccacgcact
     6481 cgctgggcgg ccgctggcga tcggcgtcgg tagcagccgg cacgacggcg ctgctatttc
     6541 gtgaactggc tctgccgtat tgcggcatcg cctgcctgag cgccgcctgg aatcgccgct
     6601 ggcgcgaatg cgcggcgtgg gcggcgggga tcgccctgtt cttcgcgttc ttcgcctggc
     6661 acgtgtcgca ggtccgcggg caactggcgg gaatggaagc tgccgcgccg ggcgcggggc
     6721 tggcgcagtg gctgcgcctg ggcggcctgg atttcgtgct gctcactacg cggatgaacg
     6781 gcctcttgtt tgccgcgccg gccgcactgg tgtggctgta cctgctggcg tcgctggtgg
     6841 ggctggccca ccgcggcgac gaaaccagcc ggttggcctg cctggcggcg ctggcctacc
     6901 tgctggcctt cgcgatcctc ggacgcccgg agaacttcta ctggggactg cttcccgcgc
     6961 cgctcttggc ctgggggacg gccagcgcgc cggcggccct ggcccggctc tggcaggcgg
     7021 cccaagcaga tattccagcc gtgccgtttg taacgccgag cccggctggc gctggacagc
     7081 cccccgcgga gacgtagatt ttcgcggcct agaaaatttt gcggagacgt tccatgagcc
     7141 agatctcctt tcttagcagc ctgtcggctt tgcccagtgc agaccaggta cgcgagtatc
     7201 ccggcagtga tgtgccgccc gagattcagg ccctgggctc gccgcgagaa gtccaccgtc
     7261 ccggctcgtt cgccgccctg gcaaactttc gctaccccct tgccgcgctg gtggtggcgg
     7321 cgttcggcgg cctggcttac atcctgtacc aggatctggc cagcaagaat cagctggatg
     7381 gcaacgcgct gtttgtgttc gcggtgatcg gtgcgctggc tgtgtcgttc gccgcactga
     7441 tcctccgaat ccctgtcacg aactcgacag tcttcttcta ttcgcgggcg gccgtcgttg
     7501 cccagaaaga caagctcacg ctcatcccct ggaaacagct gctctatcgc cgcagcacga
     7561 tctccacgcc ggaggggcgg cagttttatt gcggctggct ggagagcttc gacacgttcg
     7621 aagaacggat ctgggaatac tcggccgagc actggctgcc ggaggccgag gcaaagatca
     7681 aagccggcga gaccgtcaag gtgggcgagc tggcgatcag tgcgaaggac atttcctacc
     7741 agggccgcac gacaacttgg gacagggtga cgcggctgct gctgctggtc gggcgcgtct
     7801 atcagctcca catctcgacg aaagatacgt ggtgccagtg ggcgacgatc gacatgcacc
     7861 agattcccaa cgcccgcgcg gtgcaacagc tgattacgaa catttgcccg ccgcacctgc
     7921 tgacgacggc cgactgaaaa gcacgagcgc cggccaacct ttcgatcagc cggcgctcgc
     7981 cgaatcggta tcgatcgccc gcgggctaaa tcagtgtgca gctgcggcgg cccgtcattc
     8041 gctaagcaag cggcatgccg ccagcgccgc gaagctcgcg cgctgcaaat ctctagttgt
     8101 tcacctgaac gcgtccgcag ctcgcgcccg cgccgcatga catcgtgtcg caaacggcgt
     8161 cgcgcctgac gccggtacac gtcgccgagt gggtgcaaat gccgcgcggc gtacagtatg
     8221 atgcggcggg taattgctct ccctcgttac gcgactggat tgaccatgcc caagcacatt
     8281 cgctctgcca cgagacgcac tttcctccaa gcggccgcgg ccgttgccgc gattccgctc
     8341 gctcctacga tcctcggtgc tcaagacaag gccggcacga agcggccggt gatcgggagc
     8401 ggcgagcaca cgtacgagtg cgtacacaat tggggcgagc tgcccagcca cgtcaaatgg
     8461 ggcgagaccc acggcgtggc cgtcgatgcg gccgggctca tctatatcaa gcaccgcaac
     8521 aacgcggccg acccgatgga tgcgatcgtg gtcttcgatc cggccggcaa gttcgtgcgc
     8581 agcttcggcg acgaatacaa cggcggcggc cacggcatcg acatccgcaa agagggcaac
     8641 accgagttcc tctacctctg cgacgtcaag cacaacctcg tcaccaagac gacgctcaag
     8701 ggcgaattcg tctggacgaa gggccggccg ccggagccgg gcgtctacaa ggaccccgcg
     8761 gtaaagtaca gcccgaccaa cgtggcgttc gggccggatg gcgggttcta catcggcgat
     8821 ggctacggct cacactacat ccatcaatac gacaaggacg ccaactgggt ccgcacgtgg
     8881 ggcggcgggg gcaccgagcc cggcaagatg cgaacgccgc acggtctgtg gctcgacaac
     8941 cgccccggcc gcgagcccac gctcgtggtc gccgaccgcg cgaacgcccg cctgcaatac
     9001 ttcacgctcg acggccagca cctgagcttc gtgaatgagg tgagcttccc cgctcacttc
     9061 gacatccgcg gcgacgtgct gctggtgcct gacctgcacg ctcgcgtgtc gctgttcggc
     9121 aaagacaaca aggtgattgc ccacc
//
LOCUS       DASRJB010000372         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1230665, whole genome shotgun sequence.
ACCESSION   DASRJB010000372 DASRJB010000000
VERSION     DASRJB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230665"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFB80_09805"
     CDS             complement(<1..301)
                     /locus_tag="VFB80_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34104.1"
                     /translation="MNLTRRELLESLGIAAGLVTAGYCATAKGYAANEEITVGCIGTG
                     GRCRRLMQSLGTIPGVKIAAACDVWDFHLAEGAKLAQPQAFATKDYRALLDRKDID"
     gene            complement(298..681)
                     /locus_tag="VFB80_09810"
     CDS             complement(298..681)
                     /locus_tag="VFB80_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HZN34105.1"
                     /translation="MQDASTTLAQLREIVRRFVEERDWRQFHSPKNLSMSLAIEAAEL
                     MEHFQWIDAAESRRIGDDPAKLAAVREEIADCCCYLLALANELEIDLAAAVRDKMVKN
                     AAKYPAEQSRGRYAPPELPGQWSRS"
     gene            complement(683..1741)
                     /locus_tag="VFB80_09815"
     CDS             complement(683..1741)
                     /locus_tag="VFB80_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN34106.1"
                     /translation="MPSSSIDRLLADPIANYPAATVACFDPTTGGPPRRTLDEELTVA
                     FLEKLAAAGAPAVLIAASTGHGHARTVEELEQWFRVAARAKLGETVLTALLRPEDGPA
                     ANQRLAGLLAELGYAVAFVRPGRGLPADATDEQVAENMRPTITAAAAAGLAVGLYSIP
                     DVSGVRMTPAAAVLLVTQCDRVVAVKVTERSYDDSTLRFLQDPRLAPLKIVQGWDPHI
                     ARALQDGPRHDARGRQRCGVTSGPMSFAVYQYNHILAAAERGDWEEVAAAQVAVTALF
                     AAMQDDPAKFADLQRAKYIMGLGHPLTGSITPRQFEPVLRALQELPRAADRRRLVQSL
                     TLMDDGPFHYGLKAFVET"
     gene            2044..4353
                     /locus_tag="VFB80_09820"
     CDS             2044..4353
                     /locus_tag="VFB80_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34107.1"
                     /translation="MSASVRTDNPLAGTRLRSLARSLHARLFVSTAGRIFAVRGRPAH
                     LVLARRLASIASVPSAAEAPTPLELGISLADGELTDGTLQAISVTLDLWRNQLDTESR
                     RGGPRREQLVAAERRELAVLSGRLAACRQWRGRAAAGDEPAGATYLLGEVAEARLAEA
                     LGAIGEDRPPLQNWLARLVHWLAGPRAMTRFLAAAGSLAANSKPTRKSRDLLALVAAT
                     VATDGGVAPLPQRLLQAEAGHANAALRALVAQSRQPGYEALLGALAQLPHVRQPRLFA
                     AVRQLLARGIGLADVAWAAEHHLLAPLAESKLPPGRVRRLADQFAAHGLPLDPASLCN
                     IAGELASRADESLALRWLHWCDRWPAKSLPPRLRRVLRAAFSDCVLPALHRPWMTGPL
                     EQWLAAQSPLGSASLSADPQACLEQLAAWQRLIGQPAATPKSVRRLLEREQSRHGERV
                     HLARQLAANPSCGENIHRRYERLCVQHSAEPAGTAKLARAATAAAVMGAIEALRTLLR
                     RAGEEAWRLETSLALPAHPQAAPDRLACWVAEMDDAARQLLREVTAAWQATGPGYKLA
                     LAGNRQWLAQAAAAGIDVQRWLRGEHTTADIGGQPVEIGLAADPRDVFLMGDYFRTCL
                     SLGDCNQLSPLFNAHDANKQVVLAIGRLPSGKRQVVARQLIAVSSEWQLIGYRCYRVF
                     GEGTSQREACLAAIAAYCGRLARRCGLPLGDAGQPATLSSDKWLDDGTVPWHPAARSA
                     CQGVPQATSDLEPQVWLCLPCAPLVACGD"
     gene            complement(4779..>5036)
                     /locus_tag="VFB80_09825"
     CDS             complement(4779..>5036)
                     /locus_tag="VFB80_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34108.1"
                     /translation="VGSPVTGKGFGALSPLPYLEHLSLDRSAVDDRGLDSIAKLRQLR
                     SLRLYSTRVTRGRIDALRQKAPQCRVLADHGDFAPMESGDK"
BASE COUNT          730 a         1751 c         1687 g          868 t
ORIGIN      
        1 cgtcgatatc cttccggtca agtagcgccc ggtagtcctt cgtcgcgaag gcctgcggct
       61 gcgcgagttt cgccccttcc gccaggtgaa agtcccacac gtcgcaggcc gccgcgatct
      121 tcacgccggg aatcgtcccc aggctctgca tcagccgtcg gcagcggccg cccgtcccga
      181 tgcagcccac ggtgatctcc tcgttggctg cgtagccttt ggccgtggca cagtaccccg
      241 ctgtgacgag gccggcggca atgcccagcg attcgagcaa ctcgcgtcgg gtcagattca
      301 tgatcggctc cattggccgg gaagctccgg cggggcatag cggccgcggc tttgctcggc
      361 cggatacttg gctgcgtttt ttaccatctt atcgcggacg gcggcggcga gatcgatctc
      421 cagctcgttg gccagcgcca gcaggtagca gcagcagtcg gcgatttcct cgcgcacggc
      481 ggccagcttg gcagggtcgt cgccgatccg ccgcgactcg gccgcatcga tccactgaaa
      541 gtgctccatc aattccgccg cctcgatcgc cagcgacatg ctgaggttct tgggcgagtg
      601 aaactggcgc caatcgcgct cttccacgaa gcggcggacg atttcgcgca gctgggcgag
      661 cgtcgtcgat gcgtcctgca tttcaggttt ccacaaacgc cttgagtcca tagtgaaacg
      721 gcccgtcgtc catcagcgtc aggctctgca cgagccgccg gcggtcggcg gcccggggca
      781 gctcctgaag ggcccgcagc acgggttcga attgccgtgg cgtgatgctg cccgtgagcg
      841 gatgccccag gcccatgatg tacttggctc gctgcaagtc ggcgaacttc gccgggtcgt
      901 cctgcatcgc ggcaaacaag gccgtcaccg cgacctgcgc ggcggcgacc tcttcccagt
      961 cgccgcgctc ggcagcggcc agaatgtgat tgtattgata gacggcaaac gacatcggcc
     1021 cgctcgtcac gccgcaccgc tgccggccgc gggcatcgtg ccgcggaccg tcttgcagcg
     1081 ctcgggcgat gtgaggatcc cagccctgca cgatcttgag gggtgccagc cgcgggtctt
     1141 gcagaaagcg cagcgtgctg tcgtcgtagg aacgttccgt gaccttgacg gcgacgaccc
     1201 gatcgcactg ggtaacgagc agcaccgcag ctgcgggcgt catccgcacg ccgctgacgt
     1261 cggggatcga gtacagcccg accgccagac ccgcagctgc tgcggccgta atcgtaggcc
     1321 gcatattctc ggccacctgc tcgtccgtcg cgtccgccgg caggccgcgg ccggggcgca
     1381 cgaacgccac ggcatatccc agctccgcca gcagtccggc cagccgctga ttagcggccg
     1441 ggccgtcttc cggccgcagc aacgccgtca aaacggtttc gcccaatttc gcccgggctg
     1501 ctacacgaaa ccattgctcc aattcctcga ccgtccgcgc gtggccgtgg cccgtcgaag
     1561 cggcgatcag cacggccggc gcaccggctg ccgcgagctt ctcgagaaag gcgaccgtca
     1621 gctcctcgtc gagcgttcgc cgcggcggcc ctcccgtcgt cgggtcgaag caggccactg
     1681 tggccgccgg atagttggcg atcgggtcgg cgagcaagcg gtcgatcgag gagctgggca
     1741 tgggtatcag aatatcgcga acggccgggg cggtgcatcc gggagggcgc gaaccaatgg
     1801 ctcgcggcag gtgtccttcc cagccggact gtgctgtttt tgcggcgaac cctttcctta
     1861 cgaaccgtca tgttcgttct cttcgacaac cgcgcgcttt ttgcgtcccg ggcgctgagc
     1921 ctcgactagc tctgcgcgct cgcggcgcgc taagggttcg cttcgttttt gtgcttctcc
     1981 gtttcgcggt tcgacccgcg cgcgggcgct ggccgctcgt gcgcgcggcc gctcgattgg
     2041 actatgtctg cgtctgtacg taccgacaat ccacttgccg gaacgcggct gcggtccctg
     2101 gcccgctcgc tgcacgcccg gctgttcgtt tccaccgccg gccggatttt tgccgtccgc
     2161 ggccggcccg ctcacctcgt gctcgcacgg cgtctggcca gcatcgcgtc cgtaccgagc
     2221 gccgccgaag ctccgacgcc gctggagttg ggcatttcct tggccgacgg cgagctgacc
     2281 gacggcacgc tgcaggcgat ctccgtgacg ctggaccttt ggcgaaacca gctcgacacg
     2341 gagtcgcgcc gcggcgggcc gcggcgggag cagctcgttg ccgcggaacg ccgcgagctc
     2401 gccgtgctgt ccggccgcct ggcggcctgc cggcagtggc gcgggcgggc ggcagccggc
     2461 gacgagccgg ccggcgcgac ttatctgctg ggcgaagtgg cggaggccag gctggcggaa
     2521 gcgctcggtg cgattggcga ggaccgtccg ccattgcaaa actggctggc gcggctggtg
     2581 cattggctcg ccggtccgcg ggccatgacg cggttcctgg ccgcggcggg atctctcgcg
     2641 gcgaactcca agccaactcg caagtcccgc gacttgctgg cattggtcgc ggcaacggtc
     2701 gccaccgacg gcggagtcgc cccgctgccc cagcgactgc tgcaggccga agccggccac
     2761 gcgaatgccg ctctgcgggc gctcgtggct caaagccgtc agccgggcta cgaggcgctg
     2821 ctgggtgctc tcgcccagct gccacacgtg cgccagccgc gcttgtttgc ggcggttcgc
     2881 cagctactgg cccgcggcat cggcctggcc gacgtcgcct gggccgctga gcaccatttg
     2941 ctcgcgccgc tggccgaatc gaagctgccg ccggggcgcg tccgccgtct ggccgaccag
     3001 ttcgcggcgc acggcctgcc gcttgaccca gccagcctgt gcaatatcgc cggcgagctg
     3061 gcgtcgcggg ccgacgaatc gctcgccctc cgctggctgc actggtgcga ccggtggccg
     3121 gcaaaatcgc tgccgccgcg gctgcggcgc gtgctgcggg ccgccttctc cgattgcgtg
     3181 ctgccggcgc tgcaccgccc gtggatgacc gggccgctcg agcagtggct cgccgcccaa
     3241 tcgcccctgg gctcggcttc gctcagcgcc gatccgcagg cttgcctgga gcagctcgcc
     3301 gcatggcagc ggctcatcgg ccagcctgcg gccacgccga aatccgtgcg gcggctcttg
     3361 gagcgcgagc agagccgtca cggcgagcgc gtgcacctgg cccggcaact ggccgccaat
     3421 ccgtcctgtg gcgagaatat tcaccggcga tacgaacggc tctgcgtcca gcactcggcc
     3481 gagccggcgg ggacggcgaa gctggcccgg gcggcgaccg cggcggcggt catgggggcg
     3541 atcgaagcgc tgcggaccct gctgcgccga gcgggtgaag aggcctggcg cctggaaacg
     3601 tcgctcgccc tccccgcgca tccgcaggcc gctccggacc gcctcgcttg ctgggtggcg
     3661 gagatggacg acgccgcgcg gcagctgctc cgcgaggtga ccgcggcctg gcaggcaacc
     3721 ggccccggct acaagctcgc gctggcgggc aatcgccagt ggctggccca ggcggccgcg
     3781 gccggcatcg acgtgcagcg ctggctgcgt ggcgagcaca ccacggcgga tatcggcgga
     3841 cagccggtcg agatcggtct ggccgccgac ccgcgcgacg tgtttctgat gggggactat
     3901 tttcgcacct gcctcagcct cggcgactgc aaccagctgt cgccgttatt caacgcacac
     3961 gacgccaaca agcaagttgt gctcgccatc ggccgcctgc ccagcggcaa acggcaggtg
     4021 gtcgcccggc agctcatcgc cgtcagcagc gagtggcagc tcatcggtta ccgctgctac
     4081 cgcgttttcg gcgagggcac gagccagcgc gaggcgtgcc tcgcggcgat tgccgcgtat
     4141 tgcggccggc tggcccgccg ctgcggtctg ccgctaggcg acgcgggcca gcccgccacg
     4201 ttgagcagcg acaagtggct cgacgacggc acagttccct ggcatcccgc cgcgcggagc
     4261 gcctgccaag gcgtcccgca agcaaccagc gacctggagc cgcaagtttg gctctgcctg
     4321 ccctgcgcgc cgctggtcgc gtgcggagat tagtgcctgc tcaattctgc ccggcgctga
     4381 tttagcttgg cacgcccgaa gtagcccttt tgttgcgaaa cgcaacagga gcagctggtg
     4441 gctggcaatc gacccgtggt tgggccttca ttcgagggca gatcgatcgc cgtaactcgt
     4501 tagctatgag atgcttgcga tcattcctgc agcattccgc cgcattgatc ctttttggcg
     4561 cgctgggtgc taagggaatg aaacgcccgg cccattccgg ctgggcacta acccttcggc
     4621 tggcccgtgg ggtctctggc gcgattcgcg gttgtggttg acttgcccct ggccactgcc
     4681 gtaatccaaa acgtttttgg gacgccagag acccccgttt tttatttctg gatgcggctt
     4741 cctcttcacg cccagggccc aaccgcacgc cacaggctct atttgtcgcc agattccatt
     4801 ggtgcaaagt caccgtgatc ggcgagcact cggcactgcg gtgccttttg gcgtaaagcg
     4861 tcgatgcggc ctctggtgac gcgagttgaa tacagccgca aactgcggag ctgcctcaac
     4921 ttggcgatag aatcgaggcc acggtcatcg acagcagagc ggtccaatga aagatgctcc
     4981 aagtacggca atggcgacaa ggcgccaaac ccttttccag tgactggcga tccaac
//
LOCUS       DASRJB010000373         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3206334, whole genome shotgun sequence.
ACCESSION   DASRJB010000373 DASRJB010000000
VERSION     DASRJB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206334"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1257
                     /locus_tag="VFB80_09830"
     CDS             142..1257
                     /locus_tag="VFB80_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34109.1"
                     /translation="MTPTRRASEGVLFLALLALPLPLAAADQAVLAELDALGARTTRN
                     EAGEVTAVDLTNAWLSDADLALLARLPQLESINLSYTKITDEGLERLAPLQRVQVLDL
                     YYAESVTDLGIAHLKHWKNLRRLNLRGTKVTSSLFEHIAGMPQLTFLDVGHSRVNDDL
                     FEVLDTLPRLAHLSFGGNKMSGAALPLLKSLPALKSLSVSGQQRTDSGLWSVAVTDFN
                     VTQIAQIGGLEVLDLGGTNLTDRGVAELAGLQNLQSLDLRGTRVTGKGLAALAGLPQL
                     CRLKLWQARGIDDAAAGALVELKSLQILEVPETSLTAAGLMQLAAHPRLEQLYIGGLA
                     LDGEQLAALRRAWPKCQISWWQKPEIENLEPRRRFGN"
     gene            1290..1913
                     /locus_tag="VFB80_09835"
     CDS             1290..1913
                     /locus_tag="VFB80_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN34110.1"
                     /translation="MRPQPLIAVTDVEASSRWYQRLLGCQSAHGGKEYERLIWRGQLV
                     MQLHSFEVHHHHGPIGDVNDEPYGNGVLLWFEIDDFDAAMDRVAELNPRIVLPRHRNP
                     PDGRGGPNHWECWLRDPDGYTVVLASPDGSAAGESNPPLRYSLLRLVLLLTVSCVALA
                     VVRNGMAAAIFAGGPLVLMLWELIQFGREMSRAPSQQTQPRIVARKI"
     gene            1965..>2908
                     /locus_tag="VFB80_09840"
     CDS             1965..>2908
                     /locus_tag="VFB80_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34111.1"
                     /translation="MSPTRRHFLQSSLAAGAAAGVLAAPAVHAQEKAKRYRTALVGTG
                     WWGMNILGEAVASEACEVVGLCDVDENQLKIAEEKLRPKTSDQPKRYGDFRELLEKEK
                     PEICIVATPDHWHPLITIAAVKSGAHVYVEKPIGHTIREGRAMVKAARDTGKTVQVGT
                     HRRVSPHNVSGIEFLKSGKAGKIGMVRAFVHYGGGPERVRPNEEPPPGLNWDMWCGPG
                     PQRPYNPAIHPKGFRNFLDYANGTLGDWGIHWMDQILMWTDEKFPKRVSSVGGRHIKG
                     PAVNGQQGQTTDAPDCQVAQFEFESFTAVWEHRQFAGN"
BASE COUNT          517 a          980 c          938 g          473 t
ORIGIN      
        1 agctgcgcca gctgaacctg taccacacgt tttataccga caagggccgc gagcagcttc
       61 gcgccgccct gccgcagtgc gaggtaatct tcgacgcccg ttcgagcgat cccaagcgcc
      121 gccgcagctg aagaggctcc aatgacaccc acccgacgcg ccagcgaggg agtgctgttc
      181 ctggctctgc tggctctgcc actccccctc gccgctgccg accaggccgt gctggccgag
      241 ttggacgcgc tcggggctcg caccactcgc aacgaggccg gcgaagtcac ggcggtcgat
      301 cttaccaacg cctggctcag cgacgccgat ctcgccctgc tggcccgcct gccgcagctg
      361 gaatcgatca acctctcgta cacgaagatc accgacgagg gcctggagcg actcgcgccg
      421 ctgcagcggg ttcaggtgct cgacctgtac tatgccgaat ccgtcaccga cctgggcatc
      481 gcccacctca agcactggaa gaatctgcgg cggctgaacc tccgcgggac gaaggtcacc
      541 agctcgctgt tcgagcacat cgccggcatg ccgcagctca cttttctcga cgttggccac
      601 agccgagtga acgacgacct gttcgaagtg ctcgacacgc tgccgcgtct ggcgcacctc
      661 tcgttcggcg gcaacaagat gagcggcgcg gccctgccgc tgctcaagag cctgcccgcg
      721 ctcaagtcgc tcagcgtgtc cggccagcag cggaccgact ccggcctgtg gagtgtcgcc
      781 gtcaccgatt tcaacgtgac gcagatcgct cagatcggcg gcctggaggt gcttgacctg
      841 gggggcacga acctgaccga ccggggcgtg gccgagctgg ccggcctcca gaatctccag
      901 tcgctcgacc ttcgcggcac gcgggtcacc ggcaagggcc tggccgcgct cgccgggctg
      961 ccgcagcttt gccgcctgaa gctctggcag gcccgcggca tcgacgacgc ggctgccggc
     1021 gcactggtcg aactgaaatc gctccagatc ctggaagtgc ccgagaccag cctcacggcc
     1081 gcgggcctga tgcagctcgc cgcgcacccg cgcctcgagc agctctacat cggcggcctg
     1141 gctttggatg gcgagcagct tgcggcgctg cgccgggcct ggcctaaatg ccaaatcagc
     1201 tggtggcaaa agcccgagat cgaaaacctc gaaccccgcc ggcgattcgg caattgaatc
     1261 tctcgacact cgactcccgc ctcccgacca tgcggcctca accgctgatt gctgtgaccg
     1321 acgtggaagc gagcagccgc tggtatcagc ggctactagg ctgccagagc gcgcatggcg
     1381 gcaaggaata cgagcggctc atctggcgcg gacagctggt gatgcagctg cacagcttcg
     1441 aggttcacca ccatcacggg ccgattggag acgtcaacga cgagccttac ggcaacggcg
     1501 tcttgctgtg gttcgagatc gacgacttcg atgcggcgat ggaccgcgtc gccgaactca
     1561 atccgcgtat cgtcctgccg cggcaccgca atccgcccga tggccgcggc ggcccgaatc
     1621 actgggagtg ctggctgcgc gatcctgacg gctacaccgt cgtcctggcc agccccgatg
     1681 gctccgcggc cggcgaatcc aatccgcccc tgcgctacag cctgctgcgg ctggtgctgc
     1741 tcctcaccgt gtcgtgcgtc gcgctggccg tcgtacgcaa cggcatggcc gccgcgattt
     1801 tcgccggcgg gccgctggtc ctgatgctct gggagctgat ccagttcggc cgagagatga
     1861 gccgggctcc gtcgcaacaa acccagccac ggatcgtagc gaggaagatc tgacacagca
     1921 tgcatcagcc gagttgacca atccattatt cccggagctt ttccatgtcc cccactcgtc
     1981 gccactttct tcagtcttcg ctggccgccg gagcagccgc gggcgtcctt gccgcgccgg
     2041 ccgttcacgc ccaggagaaa gccaagcgct atcgcacggc tctcgtgggg accggctggt
     2101 ggggcatgaa tatcctgggc gaggcggtgg cgtcggaggc ctgcgaagtg gtgggtctgt
     2161 gcgatgtgga cgagaaccag ctcaagatcg ccgaggagaa gctgcggccc aagacgtccg
     2221 atcagccgaa gcggtatggc gacttccgcg agttgctcga gaaagagaag cccgagattt
     2281 gcatcgtggc gacgcccgat cattggcatc cgttgatcac gatcgccgcg gtgaagagcg
     2341 gggcgcatgt ctacgtcgaa aagccaatcg gccacacgat ccgcgaaggg cgggcgatgg
     2401 tcaaggcagc ccgcgacacg ggcaagacgg tgcaggtcgg cacgcaccgc cgcgtctcgc
     2461 cgcacaacgt cagcggcatc gaatttctga agtccggcaa ggcggggaag atcggcatgg
     2521 tgcgggcctt cgtgcattac ggcggcggcc ccgaacgggt gcggcccaat gaagagccgc
     2581 cgccgggcct gaactgggat atgtggtgcg gccctgggcc gcagcgtccg tacaatccgg
     2641 cgattcatcc gaaggggttc cgcaacttcc tcgactatgc caacggcacg ctcggcgact
     2701 ggggcatcca ctggatggac cagattctga tgtggaccga cgaaaaattc cccaagcggg
     2761 tctcgtcggt tggcggccgg cacatcaaag ggcccgcggt gaacggacag cagggccaga
     2821 cgactgatgc accggattgc caggtggccc agttcgagtt cgagtcgttc acggccgtgt
     2881 gggaacaccg gcagtttgcc ggcaacaa
//
LOCUS       DASRJB010000374         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2072591, whole genome shotgun sequence.
ACCESSION   DASRJB010000374 DASRJB010000000
VERSION     DASRJB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072591"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1878)
                     /locus_tag="VFB80_09845"
     CDS             complement(148..1878)
                     /locus_tag="VFB80_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZN34112.1"
                     /translation="MRQLSCTLAMSGLLALVLGCEEFDASPPVAKAPAPAPKPVQKPT
                     KPKPAATSNRKGPGGQPSVEELQKELDIISKAYDASRQSGGLSPREFTPGGDPYSAAS
                     PSSGGSRFTGLAYGQTEEAAVGKIATAIKESVELSPTLVVWIIDRTPSAQRLMSQAAR
                     AAQNYYDSPDVKAWAQAANQPLLTAVVTYDEQVTFALDPPTADIQQIHTAFRSVQATE
                     SNREKTCTAIKEALEKYQSVRMRDRREVLLVVITDEAADDRPVADDVLALARRNAIPI
                     YCLGLSAPWGQTNPFAKDPKAVDPSADDSTPTFGPESVASERVDISGWGSGYSGRATE
                     LIDSGFGPYVLERICRGSRGKFFALRGSSSGGGVNRDWPTGSEARFDEGTLNKYAPDY
                     VSEAEYQKLLGENKARAALVAAARLPRVVVDGSPSANFPKQAEAQMARQLSQAQQFAA
                     KNSPPVDQLYEALAPGESDREKLTSPRWQAEYDLAMGRVCAIKARLDGYNSMIAALKR
                     GKTFKNEGSSLWVLEPADAYETESTIKRLADRAKMYLERVQREHPGTPWAAIAEEELR
                     APLGWTWRES"
     gene            2065..3456
                     /locus_tag="VFB80_09850"
     CDS             2065..3456
                     /locus_tag="VFB80_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34113.1"
                     /translation="MRRFTLFSLAFVAILAAAGGTPAAESLEKQVLGSILDYPAGPAP
                     IEELRKGGPASLDELFRIRTGVEERLKAAIAAPATEESPYHIRYAERQLARLDNIIDQ
                     VGMQRYCTRSRLYWYTDFEQAKAEATKSGKPILSLRMLGNLNEDFSCANSRFFRTTLY
                     ANEEISKFLRENYILHWKSVRPVPRVTIDFGDGRKLERTITGNSIHYVLTPDGEVVEA
                     LPGLYGPQAFLAKLSEALPVARNVAALSPEERQPLLAEYHAREFLVISDRFAADLARV
                     QAGTLATAAVAPQPAAARKAGPPAANVAAAVARPKVEIERPLLAAALPLSSDPTKLDD
                     DNVWQAIAALHAGEARIDNSSRELIRSENPNAAIAGRLAITKRRVEDPLVRLVQTLES
                     SIALDSMKNEYRFHRQIHEWLMAGNYRPDVDTLNERVYAELFLTPRSDPWLGLAPAEV
                     YTALPNAGVVQGR"
     gene            3566..>3966
                     /locus_tag="VFB80_09855"
     CDS             3566..>3966
                     /locus_tag="VFB80_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34114.1"
                     /translation="MSKTVLTVEDDSAIRRGIVDALRFKGYGVLEAGHGDLGHEMAVR
                     QQYDLLLLDLVLPGRGGLEILRAVRATRPTQPVIILTARGEESDRVEGLRAGADDYVV
                     KPFSVKELLARVEAVLRRSPGRPSDMPEVKIP"
BASE COUNT          652 a         1323 c         1311 g          680 t
ORIGIN      
        1 tcctctcatc ttgcccgccc tgaaaggccg cggcggcctg aggtgctgca tcctcgaagg
       61 gggaacgatc actgtcggcg acgaaatcgt cccgctgacc cccgactcct gactcggaac
      121 gccacagagg gcgttcccta cagcgcttca agactcgcgc caggtccacc ccagcggggc
      181 gcggagctct tcctcggcga tagcggccca gggtgtgccg ggatgttcgc gctgcacgcg
      241 ctccaggtac atcttcgcgc ggtctgccag ccgcttgatc gtgctctccg tctcgtaggc
      301 atcggccggc tcgagcaccc acagcgagct gccttcgttt ttgaacgtct tgccccgctt
      361 aagggcggcg atcatcgagt tgtagccgtc gaggcgggcc ttgatcgcac agacgcggcc
      421 catcgccagg tcgtactcgg cctgccaccg cgggctggtc agcttctcgc ggtcggactc
      481 gcccggcgcg agcgcttcgt aaagctgatc gaccggcggc gagtttttgg ccgcgaactg
      541 ctgcgcctgg ctgagctggc gggccatctg ggcctcggcc tgcttcggga aatttgccga
      601 gggactccca tcgacgacca cccgcggcag cctcgccgcg gccaccagtg ccgcgcgggc
      661 cttgttttca cccagcagct tctggtactc ggcttccgac acatagtcgg gtgcgtactt
      721 gttcaaagtg ccttcatcga accgcgcctc gctgccggtg ggccaatcgc ggttgacgcc
      781 gccaccgctc gatgagccgc ggagggcgaa aaacttgccc cggctgcccc ggcaaatgcg
      841 ctccagcaca tacggtccga agccggagtc gatcagctcc gtagcccggc cggagtagcc
      901 gctgccccag cccgagatat cgacccgttc cgaggctacc gactcgggac caaacgtcgg
      961 cgtggaatcg tcggcgctgg gatcgaccgc cttgggatcc ttggcaaagg ggttggtctg
     1021 cccccacggc gcggacaggc ccaggcaata gatcggaatc gcattgcggc gggccagcgc
     1081 cagcacgtcg tcggccaccg gtcggtcgtc cgccgcttcg tcggtaatca ctaccagcag
     1141 cacctcgcgg cggtcccgca tgcggacgct ttgatacttc tccagcgcct ccttgatcgc
     1201 cgtgcaagtc ttctcgcggt tgctttccgt ggcctggacc gagcggaacg cggtgtgaat
     1261 ctgctggatg tcggccgtgg gcggatcgag ggcgaaagtc acctgctcgt cgtaggtgac
     1321 gaccgcggtc agcagtggct ggttggctgc ctgagcccaa gccttcacgt cgggcgaatc
     1381 gtagtagttc tgggccgcgc gggcggcctg cgacatcagc cgctgggcgc tcggcgtgcg
     1441 gtcgatgatc cagacgacca gcgtcggcga cagctccacg ctttccttga tcgccgtcgc
     1501 gatcttgccg acggccgcct cttccgtctg gccgtacgcg agcccggtga aacgcgagcc
     1561 gccgctgctg gggctcgccg ccgaataggg atcgccgccc ggcgtgaatt cgcgtggtga
     1621 caggccgccc gactgccgcg aggcatcgta ggccttcgag atgatgtcga gctctttctg
     1681 caattcctcg acagagggct gcccgcccgg tcccttgcga ttgctggtcg cggccggctt
     1741 gggcttggta ggcttttgaa ccggcttggg agctggcgcc ggcgccttgg ccacgggcgg
     1801 gcttgcgtcg aattcctcgc aacccagcac cagcgcgagc agcccgctca tcgccagagt
     1861 gcacgacaac tgcctcatac ggtcctcccg ccgtgcctgc ccgtgttccc tcatcgattt
     1921 cattgtaacc cagccgcgtc cattcgttac gaaaaacccg cattgccggt ggttacaaac
     1981 gctttacacc ccacggctgg tgggagacgt aggattcgcc gaagaggcca aaagggcaag
     2041 gctttgataa caggaggcca aaacatgcgt cgattcacgt tgttcagttt ggcattcgtg
     2101 gcgatccttg cggcggccgg cggcacgccc gcggctgaat cgctggaaaa gcaggtgctg
     2161 ggatcgatcc tcgattatcc ggcgggcccc gcgcccatcg aagagctccg caagggtgga
     2221 ccggcgtcgc tcgacgagct gttccggatt cgcaccggcg tcgaggagag gctgaaggcc
     2281 gcgatcgcgg ccccggccac cgaggagagc ccctaccaca tccgctatgc cgagcggcag
     2341 ctggcccggc tcgacaacat catcgaccag gttggcatgc agcgctattg cacccgctcg
     2401 cggctgtact ggtacaccga ttttgagcag gccaaggccg aagccactaa gagcggcaag
     2461 ccgatcctga gcctgcggat gctgggcaac ctgaacgagg atttctcgtg cgccaacagc
     2521 cggttcttcc gcacgacgct gtacgccaac gaagagatct cgaaattcct gcgcgagaac
     2581 tacatcctgc actggaagag cgtgcggccg gttccgcggg tgacgatcga ctttggcgac
     2641 ggccgcaaat tggagcgaac gatcacgggc aacagcatcc actatgtgct gaccccggat
     2701 ggcgaagtgg tcgaggcgct gcctgggctc tacggcccgc aagccttcct ggcgaagctc
     2761 agcgaggcac tgccagtcgc ccgcaatgtc gcggcgctgt cccctgagga gcggcagccg
     2821 ctgctggccg agtatcacgc ccgcgagttt ctggtgatca gcgaccgatt tgccgcggac
     2881 ctggcccgcg tgcaggcggg gacgctggcg acggccgctg tggctccgca gcctgctgct
     2941 gctcgaaagg ccggtccgcc cgcggccaac gtcgcggcgg ctgtggctcg acccaaagtt
     3001 gagatcgagc gtcccctgct ggcggccgcg ctgccgctct cgtccgatcc gacgaagctg
     3061 gacgacgaca acgtctggca ggccatcgcc gcgctgcacg ccggcgaagc gcggatcgac
     3121 aacagcagcc gcgagctgat tcgcagcgag aaccccaatg cggcgatcgc cggccggctg
     3181 gcgatcacca agcggcgggt cgaggatccg ctggtgcggc tggtgcagac actggaatcg
     3241 tcgatcgccc tggacagcat gaagaacgaa taccgcttcc accggcagat tcacgagtgg
     3301 ctgatggccg ggaattaccg gcccgacgtg gatacgctca acgagcgggt ctatgctgaa
     3361 ttgttcctca cgccgcgcag cgacccctgg ctgggcctgg ctccggccga ggtctatacg
     3421 gctctgccga atgcgggcgt agtgcagggg cggtaggtca gtggtcggtg gtcggtaagt
     3481 cggtgggggt atgccgaagt ttccgaccac ggaccgaccg accacggacc gaccgaccac
     3541 cgaccaccga ccaaccgacc aaccgatgtc caaaaccgtt ttgaccgtcg aagacgattc
     3601 cgcgattcgc cgcgggatcg tcgatgcgct gcggttcaag ggctatggcg tgctggaggc
     3661 cggccacggc gatctggggc acgagatggc cgtgcggcag cagtacgacc tgctgctgct
     3721 cgacctggtg ctgccgggcc gcggcggact ggaaatcctc cgcgctgtcc gcgccacgcg
     3781 gcccacgcag ccggtgatca tcctcacggc ccgcggcgaa gagagcgacc gggtggaggg
     3841 cctgcgggcc ggggcggacg actatgtggt caagccgttc agcgtgaagg agctgctggc
     3901 gcgggtcgaa gccgtgctcc ggcgctcgcc cggccggccg agcgacatgc ccgaggtgaa
     3961 aatccc
//
LOCUS       DASRJB010000375         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2926623, whole genome shotgun sequence.
ACCESSION   DASRJB010000375 DASRJB010000000
VERSION     DASRJB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926623"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFB80_09860"
     CDS             complement(<1..332)
                     /locus_tag="VFB80_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34115.1"
                     /translation="MPAIPHSALRTPHSLVARRTFLSRTACGIGSAALASLLSSRSCG
                     AAEGLPASRGAISPLHRAPKAKRVIFLTMAGGPSHLETLDYKPQLAEMHGQPMPESFT
                     KGQPIAQL"
     gene            complement(334..2865)
                     /locus_tag="VFB80_09865"
     CDS             complement(334..2865)
                     /locus_tag="VFB80_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN34116.1"
                     /translation="MMMPARAFASALSLTALVALAASAAEPKISFTRQIKPILASRCL
                     ACHGPDEKERQADLRLDVREMAVPAVIKPGDAEHSEMIFRITTDDAESHMPPAKAKKP
                     NVSPDEVKLIRQWIDQGAEYDAHWSYMPPKQPSVPQVKRTDWPAGPIDSFILAKLEAH
                     SLTPAPPADKRTLLRRLNFDVVGLPPTPEELADFEADTSAQAYERVVDRLLASEHFGE
                     RMALYWLDVVRYGDTGGYHSDNHRDVSPYRDYVIAAFNQNKRFDQFTIEQLAGDLLPE
                     ATIEQRIASGYNRLLQTTEEGGAQPKEYAAKYAADRVRNTSVIWLASTMGCCECHSHK
                     FDPFTHQDFYRFAAFFADVAEKAVGRQDQTKLPTAEQAEQLQRLDAELGAAKAEFNAK
                     TPELTAAREKWEAEMLADPAKGLPAEVAVALKAAKDKRTPKQEEAIEAHFRKQLPELA
                     AVRDKIAALEKQRVAVDAEVTSTLVSMSVAPRTMRVLPRGNWLDDSGEIMQPGVPAFL
                     NSSPHAPREESVSRSETTTVRPTRLDLARWIVASENPLTARVFVNRLWKLAMGRGIVR
                     SLEDFGSQGTPPTHPELLDWLATDFQASGWDVKRALKQILMSRTYQQSSAPGKEALAK
                     DATNELFSRQNRFRLDAELVRDNALAVSGLLVAKVGGPSVKPYQPAGYWQYLNFPKRE
                     WENDKGESQYRRGLYTYWQRTFLHPSLAAFDAPSREECTVDRPKSNTPQQALVLLNDP
                     TYVEAARALAGRVLTESGPAAVERIEFAVRQTLTRAPRAEETRLLTALVEKHRQEFAA
                     DAAAAQKLLAVGDLKSPAALDPAELAAWTSVCRVLLNLHETITRN"
     gene            complement(2951..4354)
                     /locus_tag="VFB80_09870"
     CDS             complement(2951..4354)
                     /locus_tag="VFB80_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34117.1"
                     /translation="MTSTRLLAIRAIRGRFLCAALVTLTAAAAPAADVPKKPHVIFLF
                     ADDQRADTIAALGNPHIQTPNLDTLAKRGMVFRNAYCLGANVSAVCTPSRNMLLSGRA
                     YFRWTGAQAQPELANFPTAMKAAGYETYHHGKRGNTSPAIQALFDHNQYVVDLDDRTS
                     GEPCRTIVDEAIRFLKERRSEKPVFMYLAFSNPHDPRVAAEKYLALYERDKIPLPKNY
                     LPLHPFNNGEQFVRDELLAEFPRSEAEVRQHLHDYYAVISAMDHHVGRFFAALKELGL
                     YDNTIFVYSADHGLAIGSHGLFGKQNLYDDGMKPPLIFAGPGIPRGETQALAYLLDIF
                     PTVCDLVGSEIPAGLDGKSLKPVIEGRSAGVRGTLFLAFRDVQRAVRDSRWKLIRYPQ
                     VNVTQLFDLQNDPHEITSLADKPEHAERIKSLTAEMERWQKELGDRAPLTVASPQDPK
                     WDPSQRPPPAPKKKNKK"
BASE COUNT          666 a         1392 c         1526 g          828 t
ORIGIN      
        1 tggagctggg cgatcggctg ccccttggtg aacgattcgg gcatcggctg cccgtgcatc
       61 tcagccagct ggggcttgta atcgagcgtc tccaggtgag acggcccgcc ggccatcgtc
      121 aggaagatca cacgcttggc cttcggcgcc cggtgcagcg ggctgattgc gccgcggctg
      181 gccggcaggc cttccgccgc gccgcacgag cggctcgaca gcagcgaagc cagtgccgca
      241 gagccgatcc cacacgcggt gcggctcagg aaagtgcgac gggcgacaag cgaatgcgga
      301 gtgcggagtg cggaatgcgg aatggcaggc atcctagttc ctcgtaatcg tttcgtgcag
      361 attcaagagc acgcggcaga cactggtcca ggcggcgagc tcggcggggt cgagcgcggc
      421 gggcgatttc aggtcgccta cggccagcag cttctgcgcg gcggccgcgt cggcggcgaa
      481 ttcctgccgg tgcttttcca ccagcgccgt aagcagcctg gtctcttcgg cccgcggcgc
      541 gcgcgtcagc gtctgccgaa ccgcgaattc gatccgctcg accgcggccg ggccgctttc
      601 ggtcagcacg cgcccggcca gggcccgggc cgcttcgacg taggtcgggt cgttcagcag
      661 caccagcgcc tgctgaggcg tgttgctctt ggggcgatcg accgtgcact cctcgcggct
      721 gggggcgtcg aacgccgcca ggctcggatg caggaacgtc cgctgccaat acgtatacaa
      781 cccgcggcgg tactggctct ctcctttgtc gttttcccac tcgcgcttgg ggaagttgag
      841 gtactgccaa taaccggccg gctgatacgg cttgacgctc gggccaccga ccttggcgac
      901 caacaggccg ctgaccgcca gcgcgttgtc gcggaccagc tcggcgtcga gccggaaacg
      961 attctgccgg ctgaacagct cgttcgtggc gtccttggcc agggcctctt tgcccggcgc
     1021 cgacgactgc tgatacgttc gcgacatcag gatctgcttg agggcccgct tcacgtccca
     1081 gccgctggct tgaaaatcgg tcgccagcca atcgagcaat tccggatgcg tcggcggcgt
     1141 gccttgcgag ccgaaatctt cgagcgagcg cacgatgccc cggcccatgg cgagcttcca
     1201 caagcggttg acgaagacgc gggccgtcag cgggttttcg gaggcgacga tccaccgggc
     1261 caggtccagg cgcgttgggc ggacagtagt cgtctcgctc cgcgagacgg attcctcacg
     1321 cggagcgtga ggactactgt tcagaaacgc cggcacgcct ggctgcatga tctcgccgct
     1381 gtcgtcgagc cagttgccgc ggggcagcac cctcatcgtc cgcggcgcaa ccgacatcga
     1441 gaccagcgtg gaggtaactt cggcatcaac cgcgacccgt tgcttttcga gtgcggcgat
     1501 cttgtcgcgc acagcggcca gttcgggcag ctgcttgcgg aaatgggcct cgatcgcttc
     1561 ctcctgcttg ggcgtccgct tgtccttggc ggccttgagg gccaccgcga cttcagccgg
     1621 caagcccttc gccgggtcgg ccagcatctc ggcctcccac ttttcgcggg ccgccgtcag
     1681 ctccggggtc ttggcgttga actcggcctt cgcggctccc agctccgcgt cgagtctctg
     1741 gagctgctca gcctgctcag ccgtcggcag cttcgtctgg tcctgccgcc cgactgcctt
     1801 ctcggcgacg tcggcaaaga acgcggcgaa gcggtagaaa tcctggtgcg tgaacgggtc
     1861 gaacttgtgg ctgtggcact cgcagcagcc catcgtcgag gccagccaga tcaccgaggt
     1921 gttccgcacg cgatcggcgg cgtacttggc cgcgtattcc ttgggctgag caccgccctc
     1981 ctcggtcgtt tgcaacaggc ggttgtagcc cgaagcaatc cgctgctcga tggtggcctc
     2041 gggcaagaga tcgccggcga gctgctcgat cgtgaactgg tcgaaacgct tgttctggtt
     2101 gaaggccgcg atcacgtagt cgcggtaggg gctcacgtcg cggtgattgt cgctgtgata
     2161 gccgccggtg tcgccgtacc gcaccacgtc gagccaatag agcgccatcc gctctccaaa
     2221 gtgctcagag gccagcagcc gatcgacgac cctttcgtaa gcctgcgcgc tggtgtcggc
     2281 ctcgaaatcg gccagctcct cgggcgtcgg cggcaggccg acgacgtcga aattcaagcg
     2341 gcggagcagc gtccgcttgt cggccggcgg cgcgggcgtg aggctgtggg cttcaagctt
     2401 ggccaggatg aagctgtcga tcgggccggc cggccaatct gtccgcttga cctgaggcac
     2461 gctcggctgc ttgggcggca tatatgacca gtgggcgtcg tattccgcgc cctggtcgat
     2521 ccattgacgg atcagcttga cttcgtccgg cgagacgttc ggcttcttcg ccttcgccgg
     2581 cggcatgtgc gattcggcgt cgtctgtcgt gatccggaag atcatttcgc tgtgctcggc
     2641 gtcgcccggc ttgatgaccg ccggcaccgc catctcgcgc acgtccagcc gcaggtcggc
     2701 ctgccgctct ttctcgtccg gcccgtggca ggccaggcag cggctggcca ggatcggctt
     2761 gatttgcctg gtgaagctga tctttggctc ggcggccgaa gcggctagcg ccaccagggc
     2821 cgtcagggag agcgccgaag cgaaggcacg cgcgggcatc atcatgggcg ttccgagtcg
     2881 taggcggtgg ggggacggcg cagcagcgcc cggcgggaaa actcatctta aaactgccgt
     2941 gaactcgccg ctacttcttg ttcttctttt tcggcgccgg cggcggacgc tggctgggat
     3001 cccacttggg gtcctgcggg ctggcgacag tgagcggggc cctgtcgccg agctcctttt
     3061 gccaccgctc catttcggcg gtgagcgact tgatccgctc ggcgtgctcc ggcttgtcgg
     3121 cgaggctggt gatttcgtgc ggatcgttct ggaggtcgaa gagctgcgtc acattgactt
     3181 gcgggtagcg gatcagcttc cagcggctgt cgcggacggc tcgctgcacg tcgcgaaagg
     3241 ccagaaacag cgtgccgcgc acgccggccg agcggccttc gatcaccggt ttgagactct
     3301 tgccgtcgag gccggcggga atctcgctgc cgaccagatc gcagaccgtc gggaaaatgt
     3361 cgagcagata ggccagcgcc tgcgtttcgc cccgagggat tcccgggccg gcgaagatca
     3421 gcggcggctt catgccgtca tcgtagaggt tctgcttgcc gaatagcccg tggctgccga
     3481 tcgccaggcc gtggtcggcg gaatagacaa agatcgtgtt gtcgtagagg ccaagctcct
     3541 tcagcgcggc gaagaaccgg ccgacgtggt ggtccatcgc ggaaatcact gcgtagtagt
     3601 catggaggtg ctggcggacc tcggcctcgc tccgcggaaa ctccgccagc agctcgtcgc
     3661 gcacgaactg ttcgccgttg ttgaagggat gcagcggcag gtaattcttg ggcagcggaa
     3721 tcttgtcgcg ctcgtacagc gcgaggtact tttcggcggc gacgcgcggg tcgtgcgggt
     3781 tgctgaacgc caggtacatg aacaccggct tctcgctgcg gcgttccttc aggaagcgga
     3841 tcgcctcatc gacgattgtg cggcagggct cgccgctggt gcggtcgtcg agatcgacca
     3901 cgtactggtt gtggtcgaaa agggcctgga tcgccggcga cgtattcccg cgcttgccgt
     3961 ggtgataggt ctcgtagccg gcggctttca tcgcggtggg gaagttcgcc agctcgggct
     4021 gcgcctgcgc tccggtccag cggaagtagg cccggccgga aaggagcatg ttgcggctgg
     4081 gcgtgcagac cgcgctcacg ttcgccccca ggcagtaggc gttgcgaaaa accatgccgc
     4141 gcttggccag cgtatcgagg ttgggcgtct gaatgtgcgg gttgcccagc gcggcgatcg
     4201 tgtcggcccg ctgatcgtcg gcgaagagaa aaatgacgtg cggcttcttc ggcacatccg
     4261 cggcgggcgc ggcggcggcc gtcagggtca ccagcgccgc gcagaggaag cgaccgcgga
     4321 tggcacggat cgcgaggagt cgagtagagg tcatcttttt ttgtgagtcg tttaacccgg
     4381 agccggaggc gacggcggct agtggagttg tg
//
LOCUS       DASRJB010000376         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4054280, whole genome shotgun sequence.
ACCESSION   DASRJB010000376 DASRJB010000000
VERSION     DASRJB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054280"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /gene="hisH"
                     /locus_tag="VFB80_09875"
     CDS             <1..333
                     /gene="hisH"
                     /locus_tag="VFB80_09875"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZN34118.1"
                     /translation="GGEFEGLGILRGKCVRFDLPPEMKVPHMGWNRGSFLRPAPPLAG
                     IADGAFFYFVHSYHVVPEDRSLIAIEAEYGRPFCAAVWRENLFATQFHPEKSQADGLR
                     ILKNFAEM"
     gene            445..1176
                     /gene="hisA"
                     /locus_tag="VFB80_09880"
     CDS             445..1176
                     /gene="hisA"
                     /locus_tag="VFB80_09880"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZN34119.1"
                     /translation="MQIWPAIDLRGGKCVRLRQGDYQRQTVYGDDPAEMARRFVREGA
                     TCLHLVDLDGAKDGSSANLPAALAILAAVEIPCELGGGIRDEATIQRLLNLGLSRLVI
                     GTRALKEPDWFRGVCRRFPGQLALGIDAKNGLVATDGWLQTSTTSAVELARQFAGEPL
                     AAVIYTDIAKDGMLQGPNLAAMGEMNAAAECDVIASGGVTTAEDVRKLAALGLAGCII
                     GRALYEGTLTLPAALAAAREGSAVG"
     gene            1263..3356
                     /locus_tag="VFB80_09885"
     CDS             1263..3356
                     /locus_tag="VFB80_09885"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN34120.1"
                     /translation="MAKHKVEDIRNIALCGHGASGKTSLADKLLVKTGAVSGEPSVDA
                     GTSICDFDAEEKAHKRTIESSIIHFEHGGKRFNVVDTPGYADFIGQTIGALRGVDTAV
                     IVINAHSGIEVNTRRVFKEAEKAGVGRIIIINKLDEHNLDFPALIERIRDTFGNQCVL
                     FNVPIGHGADFKGVASTLKPGEAKGALISPAEIHTSLIESIVEVDEELMMKYFEGEEP
                     SHEQLTGLIVQAVAKGTLVPILCCSAKTGVGLAELLDGLLACSLPPSAVPRTAKKDGA
                     EFEIKPDPAGPLVAQVFKTRIDPFVQKLSYIRMYSGTMKKDETVPTTSSRKGTKLGPL
                     LEVQGGETKPIDFASAGEIVAIAKMEDLHTGTSLGVAELPKIAFPTPMVGLAVTPKAR
                     NDEAKLSVSLHKIVEEDSTFKLDRDAQTKELVMTGMSELHLQIIRERLHRRDKLDVDT
                     KPPKIAYRETIQTTAEGSYRHKKQSGGRGQFGEVHIRMFPFPRGTKPEEYCVKDRFPH
                     FKEFHYDDHHNFVWINSVVGGTIPSNFLPAVEKGFKDRMEKGVIAGYRVQDVAVEVHF
                     GKYHDVDSSEAAFKIAGSMAFRNVFQQARPALLEPVVKMEITVPESNVGDVYSDMSSR
                     GGRVQGSDSAGGGYQTVFAEVPLREVTTYARTLSSMTGGQGSFAMEFAHYDVMPGNIQ
                     QEVISKATLHPEEEE"
     gene            complement(3450..>4472)
                     /locus_tag="VFB80_09890"
     CDS             complement(3450..>4472)
                     /locus_tag="VFB80_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34121.1"
                     /translation="DFLDPAAPSLARTLKAAGYATAHFGKWHLGGGRDVTDAPKFAAY
                     GFDEHAGTWESPQPHADITAKDWIWSPEDKVKRHDRTAFFVDRALDFLKRHHDQPCYI
                     NLWPDDTHTPWVPSDEQVGPQPATLPEQQRKFRAVLVELDRQVGRLLDGIKELGLDER
                     TLVLFTSDNGALPTFGGERSKPYRGGKLSLYEGGIRMPFIARWPGRVPAGRTDDRTVL
                     AAVDLFPSLCKLAGAALPEGAAFDGEELSGALLGTPAARRQPLFWEYGRNETAFAYPQ
                     GRDRSPRLAIREGRWKLLVNEDGSGPELYDLQLDAGETKSVAEQQLELTRRLSDAAVT
                     WRKSLP"
BASE COUNT          840 a         1473 c         1420 g          739 t
ORIGIN      
        1 gggggcgagt tcgaaggcct gggcattctt cgcggcaagt gcgtgcggtt cgatctgccg
       61 cccgagatga aggtgcctca catgggctgg aatcgcggca gcttcctgcg gcccgcgccg
      121 ccgctggccg gcatcgccga cggggcgttc ttctatttcg tgcactccta ccacgtcgta
      181 cccgaggatc gcagcctgat cgcgatcgag gccgaatacg gccgaccgtt ctgcgccgcc
      241 gtgtggcgcg agaacctgtt cgccacgcag ttccacccgg aaaaaagcca ggccgacggc
      301 ctgcggattc tgaagaactt tgcggaaatg taatagctgc cgcgttgatt ccctcgctgg
      361 cgcttcgggt tgctttcgcc ggtgggctgt gccatattcc aaaactcacc ggtccttaac
      421 cgtgtgcgaa tgtggtgggc gacgatgcag atttggccgg cgatcgatct ccgcggcggc
      481 aagtgcgtgc gcttgcggca gggcgactac cagcgccaga cggtctacgg cgacgacccg
      541 gctgaaatgg cccgccgctt cgtccgcgaa ggcgcgacct gcttgcacct cgtcgatctg
      601 gacggggcca aggacggcag cagcgccaac ctgccggccg ccctcgcaat cctggcggct
      661 gtcgagattc cctgcgagct gggcggcggt attcgcgatg aggccacgat ccagcggctc
      721 ttgaatctcg gcctgtcgcg gctggtgatc ggcacgcggg cgctcaaaga gccggactgg
      781 tttcgcggtg tctgccggcg gtttccgggg cagctggcct tgggtatcga cgccaaaaac
      841 ggcctggtcg ccaccgacgg ctggctgcaa acgagcacga ccagcgcggt cgagctggcc
      901 cgccagttcg ccggcgaacc gctggcggcg gtcatctaca ccgacatcgc caaggatggg
      961 atgctgcaag gtcccaacct cgcggcgatg ggcgaaatga acgcagccgc cgagtgcgac
     1021 gtaattgcct ccggcggcgt gaccacggcc gaagacgtac gcaagttggc cgccctcggc
     1081 ctggccggct gcatcatcgg ccgggcgctt tatgaaggca ctctgacgct acccgcagcg
     1141 ctggcggcag cgcgggaggg gtctgccgta ggataagcgt cctcgcttgt ccatcccaat
     1201 taccaattca agcctgagca agcggggacg cttgctctac gaacgactaa aggacaacgg
     1261 gcatggcgaa gcacaaagtg gaagacatcc gcaacattgc actctgcggc cacggcgcct
     1321 cggggaaaac gtcgctggcc gacaagctgc tggtcaagac gggcgcggtt tccggcgagc
     1381 cgagcgtcga tgccggcacc agcatctgcg acttcgacgc cgaggagaag gcgcacaagc
     1441 ggacgatcga gtcgagcatc atccacttcg agcacggcgg caagcggttc aatgtcgttg
     1501 atacgccggg ctatgccgac ttcatcggcc agacgatcgg ggccctccgc ggcgtcgata
     1561 ccgcggtgat cgtcatcaac gcccacagcg gcatcgaggt caacacccgc cgcgtcttca
     1621 aggaagccga aaaggccggc gtggggcgaa tcatcatcat caacaagctc gacgagcata
     1681 acctcgactt cccggccctg atcgaacgga ttcgcgacac cttcggcaac cagtgcgtgc
     1741 tgttcaacgt gccgatcggc cacggagccg atttcaaagg cgtcgccagc acgctcaagc
     1801 ccggcgaggc caagggcgcc ctcatcagtc ccgccgagat tcacacctcg ctgatcgagt
     1861 cgatcgtcga ggtcgatgaa gagctgatga tgaagtactt cgaaggcgag gagccgtcgc
     1921 acgaacagct caccggcctg atcgtgcagg cggtcgccaa gggcacgctg gtgccgatcc
     1981 tttgctgctc ggccaagacg ggcgtcggcc tggccgaact gctcgacggc ctgctggcct
     2041 gctccctgcc tccgtcggct gtgccgcgga cggcgaagaa ggacggggcg gaattcgaga
     2101 tcaagcccga ccccgccggg ccgctggtgg cccaggtctt caagacccgc atcgacccgt
     2161 tcgtccagaa gctgagctac atccgcatgt atagcggcac gatgaagaag gacgagacgg
     2221 tgcccacgac cagctcgcgc aagggcacga agctcggccc gctgctcgag gtgcaaggcg
     2281 gtgagaccaa acccatcgat ttcgcatcgg cgggggaaat cgtcgccatt gccaagatgg
     2341 aagacctcca tacgggtact tcgctgggcg tggccgagct gcccaagatc gccttcccca
     2401 cgccgatggt cggcctggcc gtcacgccca aggcccgcaa cgacgaagcc aagctttcgg
     2461 tctcgctgca caagatcgtc gaggaagact ccaccttcaa actcgaccgc gacgcccaga
     2521 ccaaagagct ggtgatgacc gggatgagcg aattgcacct gcagatcatc cgcgagcggc
     2581 tgcaccgccg cgacaagctc gacgtcgata ccaagccgcc caagatcgcc taccgcgaga
     2641 cgattcagac aaccgccgaa ggcagctacc gccacaagaa gcaatccggc ggccgcggcc
     2701 agttcggcga ggtccacatc cgcatgtttc cattcccccg cggcaccaag cccgaggaat
     2761 actgcgtcaa ggatcgcttc ccgcacttca aggaattcca ctacgacgac catcacaatt
     2821 tcgtctggat caactcggtc gtcggcggca cgattcccag caacttcctg cctgccgtcg
     2881 aaaagggctt caaggaccgc atggagaaag gcgtcatcgc tggctacagg gttcaggacg
     2941 tggctgtcga agtgcacttc ggtaagtacc acgacgtgga cagctccgaa gccgcgttca
     3001 aaatcgccgg ctcgatggcg ttccgcaacg tcttccagca ggcccggccg gccttgctgg
     3061 aaccggtcgt gaaaatggaa atcacggtgc cggagtcgaa cgtcggcgac gtctacagcg
     3121 acatgagcag ccgcggcggc cgcgtgcagg gcagcgactc ggccggcggc ggctaccaga
     3181 cggtgttcgc cgaggtcccg ctccgcgagg tcacaaccta cgcccgcacg ctctccagca
     3241 tgaccggcgg ccagggtagt tttgccatgg agttcgccca ctacgacgtc atgcccggca
     3301 acatccagca ggaagtcatc agcaaggcca cgctccaccc ggaggaagag gagtaggcca
     3361 gatttgaagc cgcggatttg cgcggatgac acggatcgaa cgcaccttgc tccatccgtg
     3421 tcatccgtgg taatccgcgg ctaatctcct caaggcagtg attttcgcca ggtcacagcg
     3481 gcgtcgctca gtcgccgtgt gagctccagc tgctgctcgg cgacactctt cgtttcgcct
     3541 gcgtcgagtt gcaggtcata aagctccgga ccgcttccgt cctcgttgac gagcagcttc
     3601 caccgtccct cgcgaattgc gaggcgaggg ctgcggtcgc ggccttgcgg ataggcaaac
     3661 gccgtttcat tgcggccgta ctcccaaaac agcggttgcc ttcgcgcggc cggcgtaccc
     3721 aaaagggcgc cgctcagctc ttcgccgtca aacgccgcgc cctcgggcag tgccgcaccg
     3781 gccagcttgc acaggctggg aaagagatcg acagccgcga gcacggttcg atcgtccgtc
     3841 cgcccggccg gcacgcggcc gggccagcga gcgatgaacg gcatgcgaat gccgccttcg
     3901 tagaggctca gcttgccgcc gcgatacggc ttgctccgct cgccgccgaa cgtcggcaga
     3961 gcgccgttgt cgctggtaaa cagaaccagc gtgcgttcat ccaggcccag ctccttgatg
     4021 ccgtccagca atcgcccgac ctggcggtcc aattcgacga gaaccgcgcg aaacttccgc
     4081 tgctgctcgg gcaacgtcgc cggctgcggg ccgacctgct cgtccgaggg cacccagggc
     4141 gtgtgcgtgt cgtcgggcca caggttgatg tagcagggct ggtcgtggtg ccgcttcaag
     4201 aagtcgagcg cccgatcgac gaagaacgcg gtgcggtcgt gccgcttgac cttatcctcc
     4261 ggcgaccaga tccagtcctt ggccgttatg tccgcgtgcg gctgcgggct ctcccacgtg
     4321 cccgcgtgtt cgtcaaagcc ataggccgcg aacttcgggg catccgtcac gtcgcggccg
     4381 ccacccaggt gccacttgcc gaaatgcgca gtggcatagc cggcggcctt gagagtgcgg
     4441 gccagcgacg gtgcggctgg gtcgagaaag tc
//
LOCUS       DASRJB010000377        17449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4054408, whole genome shotgun sequence.
ACCESSION   DASRJB010000377 DASRJB010000000
VERSION     DASRJB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054408"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VFB80_09895"
     CDS             complement(<1..1343)
                     /locus_tag="VFB80_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34122.1"
                     /translation="MATARVSPVDSKWRLRFSLGGMMFTFVLGVSIGLAYWRSEKVSF
                     AGAMLASFTTWLVIGLFQRISCELPQLNQFRGLPLELQWGLLFRTLIPVWSLVLLAMV
                     GACEVARKFNQLASSWPVSLGNVLFYLAIIGVYWQPQAKPAEQPWRVQSTRAIVSLIL
                     LIIGVYWTAAILSGGYEMFQSVYMGIHRMELAQPTTWAGRPFQSAPLTFSSAAREQMD
                     SQLAWAVILQAIASGASLLMFCFWRSRRRLWLVFFVLWIGCLAAAMPFVRAAWSVGPS
                     ELLPWPMAPIFSLRWEEMALFAVLVASVVTAASLQLTCVPVIDEVLVSGKGSSQPASA
                     FHETLGVIGFGLCACVIATGRFIVTNVGSLAWSASAPSWMTWPQAIWVATVRICEDYA
                     IYPPQEIIVLATAVILLRCLVRRCRWPQSSLVWFIEPQKFIALWIVLLINIATIAP"
     gene            complement(1541..2200)
                     /locus_tag="VFB80_09900"
     CDS             complement(1541..2200)
                     /locus_tag="VFB80_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34123.1"
                     /translation="MRRSRLPSGTWRCKDAHKARGRQEYQEIPRNVHSFAAPGRIAFT
                     LAALLAVMTLAALFCVALARPTRFWSEAVVLLVLVGLLTSVPAIVYRTGPARAFAVGF
                     LVFTLGFFLAALISRERLFRDPGGRASDQFVSSHLGTWLFAKIHPNNFQPLTSMTGLG
                     MSIGFDSGTGSTITMVPDGGTVTMMPGRGMTMTLPKYDPARFLEIVHAAAPSSPASPA
                     E"
     gene            complement(2193..2519)
                     /locus_tag="VFB80_09905"
     CDS             complement(2193..2519)
                     /locus_tag="VFB80_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN34124.1"
                     /translation="MPKYLFIFRESTEKRAQPPAEELQALQMAWYEWMQKYQAAIVGG
                     DGLLRTGRVLKAGLVTDGPYAETKEIIASFSIIQADDYNTAVEIARACPGDCHVIEIR
                     EMGGYA"
     gene            complement(3405..3977)
                     /locus_tag="VFB80_09910"
     CDS             complement(3405..3977)
                     /locus_tag="VFB80_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN34125.1"
                     /translation="MTTFTLDTDKTELIRLVRAAQKGDRAAFGTLFERFERHVMAIAL
                     RRLGDFGEAQELCQDVFIQAMKKIRQLREPEAFAGWLRSITNRMAINRLVRKAPDHTV
                     EAETLDFACAETRTPLANALEGERAREVRKGLKQLGRLDRDTLVAFYVRGQSLVEMAD
                     SFDAPVGTIKRRLHVARKRLAKHVEAVAAV"
     gene            complement(4751..6469)
                     /locus_tag="VFB80_09915"
     CDS             complement(4751..6469)
                     /locus_tag="VFB80_09915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN34126.1"
                     /translation="MPPEKPKILLVDDDPAMLRLLGKWLESDGYEVHCAHDGRQAREL
                     IELARPSILIPDWDVPELDGVELMRWVRGLDFGHYIYTIFLTARGDSADMLSGLEAGA
                     DDFLKKPVDRNELTARLRAGARFAELEHRLSAIANTDAITGLANRRTLFDDLGREWRR
                     AQRYNAPLACVMLDIDFFKRINDLHGHPAGDEVIRQFGRVLSESVRASDIAGRYGGEE
                     FCIVLPETTEQQALKWADRVRGRLAELRFPLGHENLAVTASFGVAQRLADTTSPEQLV
                     DMADQALVVAKRSGRDRVVAYQSLTQTAVFGSHDGDPASILRHISARDVMSSIVAPLN
                     QNHSVATAANFFLRLRVTSAPVVDERGQLVGILSEKDLMAIMLGPNWWTTPIKDVMKR
                     NVVCYEEDTPALAIFEFLSRVVIRGVVIVRQGSPTGLITRGCLLRFLISLLAARRVEG
                     VFGEVDAAACKVLERMGNQPPKDRIAQTVRSLAAEACDMEWRLTQPDAELLPCVVGGA
                     SRMQEMVIDLLAISRFANEGFPSGRGSGLLPKLIVKQGLTTSDPSNSAGGSSASIDPD
                     VLARIS"
     gene            6820..7683
                     /locus_tag="VFB80_09920"
     CDS             6820..7683
                     /locus_tag="VFB80_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34127.1"
                     /translation="MVRLARRGFTLVELLVVIAIIGMLVALLLPAVQSAREAARRMQC
                     SNNLKQITLAVHNYHDALLYLPPGSTNPDLSGSSGLAAILPYLEQASAYSLYDFSKGN
                     SDPANLRSVSQKIKTYLCPSCVFARSVPIGGCDANDRAPGTYAFSTNTGDPYSLSNGA
                     IANAISPQTRLASITDGTSNTFLAGEAHWAFKDYLFSSGPCAGQVRGGFSYWSSPYPL
                     ATVFTTRGPFNAQSMSGDSLRLASFRSSHAAGVNMTNVDGSVRFVQQSIDHTLLDAGA
                     TRDGGEALALP"
     gene            7761..8219
                     /locus_tag="VFB80_09925"
     CDS             7761..8219
                     /locus_tag="VFB80_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34128.1"
                     /translation="MRSRVLCGQFLCLLFAAVVGCGESGPARAPITGQVTVGGQPLAK
                     GRILFIPAAPNQGPVASAAVRDGSYRIPQVEGPVVGQNRVEVEAELNLGFAIDDEAAF
                     AKRGGRPLPANPIPPQYNRDSKLTVEVEAGDENRFDVTIPSARHAAARPQ"
     gene            8316..9311
                     /locus_tag="VFB80_09930"
     CDS             8316..9311
                     /locus_tag="VFB80_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34129.1"
                     /translation="MSLPSALLTVGFVAAACLLAGAAHAADNVQISELPPLEKPVTSF
                     GAAVADGYLYAFGGHLGSPHEYSGDLQSRELLRLNLKQPQKWELVGEVPRRTGLAMVS
                     YGGKLYRVGGWEARKSAEGKWELFSTSDFARFDPKDGKWEDLAPLPHGRSSHDAAVLG
                     SRLYVIGGWELGDKTGGQWHETAYVCDLADARPQWKEIAKPPFNRRALAVAGYAGKVY
                     AIGGMDDSNEVTLGVDIYDPQTNAWSKGPAIPGQGFDGFGSSAIGTKAGLFVTSASGS
                     LFRLNDSGKSWDEATKLKHARTFHRLVADGDQRLLAVGGTSKGTKVPQVELIELK"
     gene            9338..10042
                     /locus_tag="VFB80_09935"
     CDS             9338..10042
                     /locus_tag="VFB80_09935"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HZN34130.1"
                     /translation="MTRLASFVAVGALLLAAAPARAHFVWVSVEKDSSGKPAAHVWFS
                     ELAEPDSAELLDRITSVKVTSRTAGGKPAAVAVAKQVQGNGGALVGAIPAGTPALSAH
                     IKYGVITRGESTFLLQYSAKYLDAAAQDFKALARDESLPLDVVPQAEASGYVLEVLFQ
                     GKPAAGAEIVVYDPTAAQLDLKTDDKGRCSLAAAKPGLYSIRARWIVKEAGKEQDKEY
                     PQVNYYSTVTLRVPSK"
     gene            10120..10314
                     /locus_tag="VFB80_09940"
     CDS             10120..10314
                     /locus_tag="VFB80_09940"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HZN34131.1"
                     /translation="MNELTQPEASGPSEPADAANRPAMPREAWRSEELLGERTEVLIL
                     HNGAVYRLRRTRQGKLILYK"
     gene            10522..13755
                     /locus_tag="VFB80_09945"
     CDS             10522..13755
                     /locus_tag="VFB80_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34132.1"
                     /translation="MSQFWLWRPANFAAPLTAQYSAFRGEPLELTLIFADLATFAAFA
                     VIPLMILYFLLRRRQVHFSRVWFLLLMYLIVGGAVHVLDACGNWEPVVHLAAALKVVV
                     AFIAWIWVIVLIPLLPKLLEARSAEEFKTLLSKHEEAEQALKETEAVYKSLIESLPLN
                     VFRKDLQGCFVDANQRFCDTLGQPLAAIVGKCDADFFPAEQVQKYRRDDTKVAESGEV
                     LEDIEFYFKPSGEKLYVQVLKAPVRDASGQIVGVQGMFWDVTERMRADEAARLSDARF
                     RKLVQSNLIGVLVARLDGSVVDANDALLQRLGYTRDDLEAGRFRWDALTPPEHRPADE
                     RAIEQLRATGVCQPWEKEYFHKDGHRVPILIGVTMLEGSETECICFIADITNQKQTER
                     ELKDAKQAADAANQAKSQFLANMSHEVRTPMNAIIGITELVLKTPLAPKQAEYLRMVM
                     QSAESLLGVINDVLDFSKVESGKVELEQQPFSLRESIGDAVKSLALRTHDRGLELALD
                     VAQDVPDWLVGDAGRLRQIVINLVGNAIKFTHQGEVVVEVRSQESGVGSRESAGNPPT
                     PDSRSPTPDSCHLQFLVRDTGIGIPPDKLEKVFEAFEQADASTTRHYGGTGLGLAIVR
                     KLIELMGGRIWVESKVGEGTRFYFTIRLQRCLLPPPARPEPKRGALKGTRALVVDDNV
                     TNRRIIEEALHSWEVVPSSCGSAAEALAALRAAYRSGAPFELLLSDVNMPGADGFALL
                     EQIRRDPSLADITAILLTSSERAEDAARCQQLRVNQRLNKPIKQSELQGAILEALGIV
                     DRPAVPDDADAAALPTTRPLRILLAEDSLVNQRLALGLLERHGHRVAIANNGREALEQ
                     SAADSFDVILMDVQMPELDGLEATRLIRERERGSQRHVPIVAMTAHALKGDRERCLAA
                     GMDEYVSKPVRERQLLTALRTVLGQEVAALPDELEPQLAPATDILNWDAALKICGGDR
                     NLLREIVLAFLEEQPQRLDEIRRAIDTSDWELLHRAAHTIKGSMRYFEARAVFDRAFG
                     LEQLAAARRLDGAEEIFTLLKQELAKLVPHLISYVQGRGGPTPASRG"
     gene            13786..14685
                     /locus_tag="VFB80_09950"
     CDS             13786..14685
                     /locus_tag="VFB80_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34133.1"
                     /translation="MPTVLVVDDSAVDRRFVGGLLSRDGRFQVEFAEDGGQALGRMRQ
                     SPPDVIVTDLQMPNRNGLELVAAVRMHHPEVPIILMTGHGSEDLAVEALHRGAANYVP
                     KPQLGERLMDAVEEALSMARADRTYDQLIACMEKCEFAFEFENDPGMIDPLVDLIQQM
                     VAGMKLTDATGRYRVGAAVKESLLNAIYRGNLEISFDQMQDTRVSLVEGKGEDLLQQR
                     SSQAPYKDRRVQVSVFLNPKEARFVIRDAGKGFDPAKVPAAGRPGSLDPQSGRGLVLM
                     RAFMDEVTFNAAGNEVTLLKRRE"
     gene            14738..15634
                     /locus_tag="VFB80_09955"
     CDS             14738..15634
                     /locus_tag="VFB80_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34134.1"
                     /translation="MATVLVVDDAAVDRKLVGGLLGRGPNLTVEFAASGDEAVERLAT
                     VQPQIIVTDLVMPGMSGLDLVARIVADYPQIPVILMTGKGSEEIAVRALKAGAASYVP
                     KSALHQLLLATVEDVLDMVRQRHTQERLMGCLKQGEFQFALGNDASLIPSLINYVQSL
                     VCSVGLCGEADVIRVCIALEEALRNAMYHGNLELTSEQREGDPVKYQELIEDRNTRDP
                     FAGRVLTVTVSVTPTSGRFTIRDQGPGFDPRKLPDPTDPENIEKVSGRGLLLMRTFMD
                     EVVFNESGNQVTMIKRLPDTPQ"
     gene            15773..16138
                     /locus_tag="VFB80_09960"
     CDS             15773..16138
                     /locus_tag="VFB80_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN34135.1"
                     /translation="MSDSKYVRTEQLGDALVVTPLFTFAAFVEPDLANEWSEVQKQVE
                     APGVKNVVVDLSAIPYFGSTVLEWMVQMWKRAKAKGGKLATCQASPIGREVLITARFH
                     QLWGICDSRAEALAWVTAK"
     gene            complement(16569..16877)
                     /locus_tag="VFB80_09965"
     CDS             complement(16569..16877)
                     /locus_tag="VFB80_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZN34136.1"
                     /translation="MHYPTELAREGNSLLITWSDGQRRRYTPRQLRDACPCATCREKR
                     SQPADPLALPTLSPALAQPLAIAGMQPMGNYAYAIAFSDGHDTGIFSFEFLRQLGAEL
                     "
     gene            17021..>17449
                     /locus_tag="VFB80_09970"
     CDS             17021..>17449
                     /locus_tag="VFB80_09970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN34137.1"
                     /translation="MFSFLVMTSGPRAGAQFILDPSRQNRIGRGLDCDVILSDPLSSR
                     VHAIIVSSDGDWWVRDAGSRNGTYVSSQKIDEARLVDGGVLKVGSTEFEFRQTLARPT
                     DATRPDHTQTIISDRSVLLGQQAGEFGLEALRDNVRAHDFL"
BASE COUNT         3298 a         5537 c         5667 g         2947 t
ORIGIN      
        1 ggggcaattg tcgcaatgtt gatcagcagt acgatccaaa gggcaatgaa cttttgcggt
       61 tctatgaacc atacgagcga cgactgcggc cagcggcatc ggcgtacaag gcaacgcaac
      121 aaaatgacgg ccgtcgcgag aacgatgatt tcttgcggcg ggtaaatggc gtaatcctca
      181 cagatcctca cggtcgccac ccagattgcc tgtggccatg tcatccacga tggtgccgac
      241 gccgaccaag ccagtgaccc gacattcgtc acgatgaagc ggcccgtcgc aatcacacaa
      301 gcgcaaagcc cgaagccgat aacgccaagc gtctcgtgaa atgcactggc cggctgggaa
      361 cttcctttgc cgctgaccag cacttcgtcg ataaccggca cacaggttaa ttgcagactt
      421 gccgccgtta caacgctggc gaccagcacg gcgaacaggg ccatttcctc ccagcgcaag
      481 ctgaagatcg gcgccatcgg ccaaggcaac aactcggagg ggccgacaga ccatgctgct
      541 cggacaaacg gcattgcggc agcgagacaa ccgatccaca agacaaagaa cacgagccac
      601 aatcgccgtc gagaccgcca aaaacagaac atcagcaaac tggctccgct ggcaatcgct
      661 tgcaggatca ctgcccacgc caactgagaa tccatctgct ctcgcgcagc tgaactgaat
      721 gtcaggggtg ctgactggaa cggccgtccg gcccacgttg tcggttgagc cagctccatc
      781 cgatgaatgc ccatgtaaac gctctggaac atctcgtagc cgccggacag tattgccgcc
      841 gtccaataga ctccgatgat gagcaagatg agcgagacga tcgcccgagt gctttgcact
      901 cgccaaggct gctcagccgg ctttgcttgc ggctgccaat agacgccgat aatcgccagg
      961 taaaacaaga cgttgccgag cgagacgggc cacgacgagg caagctggtt gaatttccga
     1021 gcgacttcgc aggccccgac cattgcgagc agaaccaaac tccacaccgg aatcaaagtc
     1081 cgaaacagca atccccactg cagctccaga ggcaaacccc tgaattgatt gagctgaggt
     1141 aactcgcagg agatccgttg aaagagaccg atcaccagcc aggtcgtgaa gctcgccaac
     1201 atcgcaccgg caaatgaaac cttttctgag cgccagtatg ccaagccaat actcacgccc
     1261 aacacaaacg tgaacatcat cccgcccagc gagaatcgca gccgccattt gctatcaacg
     1321 ggcgaaaccc gagcagtggc catgtggctg cccgaaccag gagaagtggc ggtccgccct
     1381 tgatagtagt cgttgagaac aaacctactc aagcagggcg ctcaatcgac gatcggccga
     1441 agctcgcaat cgcaatcggc gttcagtcgg ccgtgggagt caggggtcag gagtcagggg
     1501 ccaggctcgc gcttccgtgt ggcgtagagg agctgcgtca tcattccgcc ggcgatgccg
     1561 gcgaggaggg cgcagcggcg tggacgatct ccaggaaccg ggcggggtcg tacttgggca
     1621 gggtcatcgt catgcccctg ccgggcatca tggtgacggt gccgccgtct ggcaccatgg
     1681 tgatggtgct gcccgttccg ctatcgaagc cgatggacat tcccaggccc gtcatgctgg
     1741 tcaacggctg aaagttgttg gggtggattt ttgcgaacag ccaggtcccg aggtggctgc
     1801 tcacgaattg gtcgctagcg cggccgcccg ggtcgcgaaa gagccgctcg cggctgatga
     1861 gggccgccag aaaaaagccg agggtgaaga ccagaaagcc gacggcaaaa gcccgcgcgg
     1921 gaccagtgcg ataaacgatc gccggaacgc tcgtcagcag gccgacgagc acgagcagca
     1981 cgaccgcctc cgaccagaac cgcgtcggcc tggccagcgc gacgcagaag agagccgcca
     2041 gcgtcatcac ggccaagagt gccgcgagcg tgaatgcaat ccgccctggc gcggcgaagg
     2101 agtggacatt gcggggaatc tcctggtatt cctgccggcc ccttgcctta tgggcatctt
     2161 tgcagcgcca ggtcccgctc ggcaagcggg acctacgcat agccgcccat ttcgcgaatt
     2221 tcaatcacgt ggcagtcgcc cgggcacgcg cgggcgatct ccaccgctgt gttgtagtcg
     2281 tcggcctgga tgatggagaa gctggcgatg atctccttgg tttcggcata gggtccgtcg
     2341 gtcacgagtc ccgccttcag gactcggccg gttcgcagca aaccgtcgcc gccgacgatc
     2401 gccgcctgat acttttgcat ccattcgtac caggccatct gcaaagcctg gagttcctcc
     2461 gcgggcggct gggcgcgttt ctcggtggat tcgcgaaaga tgaacaagta cttcggcatg
     2521 atggttctcc acgtttcgtc aacgcggcgg actttcgccc gccttctgtg aacataacga
     2581 acgagctgca accgtttggc tagggcgaat ccatcagcga tttgcgagtc ggtagaccca
     2641 tccctctccc aaaggggcga cggcaagcct tctcccgcga gatagtcgtt cgcgcgatcg
     2701 ccgaatcgac agccgaccat cggattttac tgccagcatg cagcgtagag cgttgggtga
     2761 cacaaccggc tcggccagcc gcgcgcctct ggtcccctct ccctttggga gagggttagg
     2821 agagggtttt ggcttgccgc acgcccgtgc tcaccgacca cggcgttaga ccctcgcccc
     2881 gaccctctcc cagagggaga gggagttttc agagaatctg gattctggcg ggaataaatc
     2941 gccaggctgg cgcatctata caattgtggc aatgcgccgg cgaatacccg ttgctgccct
     3001 gaaccctgaa ccctggcagt ggaggatcgc aaggggaaaa tgtacgcctg tactgaatat
     3061 ggtgcgacgg ttaggataat tccgcggagg gctctctaac acttcaaacg ctcacgcatt
     3121 tgtcgagctt tcgcgcggcg caactcgcgg tgcggtggga agttgtgtca gggcagcagg
     3181 aaatggggca ctgaaaaatc gccagcccaa tgggaattat ccttacctcg ccgggccaac
     3241 tgcttgcggc gtcttgtgcg gcactgggcc aggagccagg agtcgggaga caggagcgag
     3301 cctgggtaaa aaaacgggcg gcggatgaat cgtcatccgc cgccctgagg ttttccaggc
     3361 gagccagtgg ccgcacgatc cgctcgcgga gcgagcggac tactttacac cgcggcgacg
     3421 gcctcgacgt gcttggccag ccgcttgcgg gcgacgtgca gccggcgctt gatggtgccg
     3481 accggggcgt cgaacgaatc ggccatttcg accagcgact ggccgcggac gtagaacgcc
     3541 acgagcgtgt cgcggtcgag ccggcccagc tgcttgaggc ccttgcggac ctcgcgggcc
     3601 cgctcgccct ccagcgcgtt ggccagcggc gtgcgggtct cggcgcaggc gaagtcgagc
     3661 gtctcggcct ccaccgtgtg atccggcgcc ttgcggacca gccggttgat cgccatccgg
     3721 ttggtgatcg agcgcagcca gccggcgaag gcctccggct cgcggagctg ccggattttc
     3781 ttcatcgcct ggatgaagac gtcctggcag agctcctgag cctcgccgaa gtcgccgagc
     3841 cggcgcaggg cgatcgccat cacgtgccgt tcgaagcgct cgaagagcgt accgaacgcg
     3901 gcccggtcgc ccttctgggc ggcccggacc aggcggatca gctcggtctt gtcggtgtcc
     3961 agcgtgaagg tggtcatagg agctcccatc gatatatgaa acagcagcaa tcgcacgcgg
     4021 cggccgaggc gagtcggccc agtgcgggcg agcgcgggaa tcaaggaatc agcggcagca
     4081 gcggcgaggg cgttttcgcg gtgatttacc gcgtggccgc tcggctggcg tccgctgtca
     4141 aagctgttgg aagatcaggc ggggccaaat gccccaggac ctaggatctc cgtaaacagc
     4201 ttgagacccg cggacgccgg caccgggacg acctttcccg ggggcgactt gtaaggtcgc
     4261 taccaccggg catcgaaccc atgcgggccg gcttttgttt gccggcaaac ggctgatcgc
     4321 cgtcgtcatg ggcccagctc ccgtgtgctg gcgcggcata gaggcgcaac gaatgggagt
     4381 cggcgcgcag ggtcacaacg ccggcaacaa tcgtgcgaac ctcattgcgg agggtcgtta
     4441 cgttgatgtt ggtattgatg acgttacgca tcggttcctc ccctgcggtc gttgcgcgca
     4501 gtgagaaaaa tggaaagaag ctcgaaacca gtttctttcg gaactgcgtt ccaatagtaa
     4561 gacgacgtat cggccgtccg cgattggaac ttggtccaaa cctgcaagcc taccttcctg
     4621 agggtctgct ggcaactgcg agcgaggcaa atcgccggtc aacaagcctc ccaggacggc
     4681 agttgcccct tagacgccgg ttcgatccag aggttcgcgg ttttccaaga atttgtccgc
     4741 gagcgccagg ctaagaaatg cgagcgagaa catccgggtc gatcgacgcc gatgaaccgc
     4801 ccgccgaatt gcttggatct gacgtcgtaa gtccttgttt gacaatgagt ttcggcaaca
     4861 ggccggatcc cctgccgctg ggaaagcctt cattggcgaa ccgcgaaatg gccagtaagt
     4921 cgatcaccat ttcctgcatt cgcgacgccc cgccgacgac gcaggggagc agctcggcgt
     4981 cgggctgagt cagccgccac tccatgtcgc aggcctcggc cgccagggag cggacagttt
     5041 gggcgatgcg gtcctttggc ggctgatttc ccatccgttc gagcaccttg caggcggcgg
     5101 catcgacttc gccgaacacg ccttcgaccc gtcgcgcggc cagcaggctg atgaggaatc
     5161 gcagcaggca gccgcgggtg atgaggcccg tcgggctgcc ctggcggacg ataacgacgc
     5221 cgcggatgac gacccgcgag aggaactcaa aaatcgccag cgcgggtgtg tcttcctcgt
     5281 agcagaccac gttgcgtttc atgacatcct tgatcggtgt ggtccaccag ttcggcccga
     5341 gcatgatcgc catcagatcc ttctcggaga gaatgccgac gagctggccg cgttcgtcca
     5401 ccaccggcgc cgacgtgacg cgcagccgca ggaaaaagtt cgcggctgtg gcgaccgagt
     5461 ggttctggtt caagggagcg acgatcgagc tcatcacgtc gcgggccgag atgtgtcgca
     5521 ggatcgacgc gggatcaccg tcgtgcgatc caaagacggc cgtctgcgtg agcgactgat
     5581 aggccacgac gcggtcgcgg ccgctccgct tggcgacgac cagggcctgg tcggccatat
     5641 cgaccagctg ctcgggcgag gtcgtgtcgg ccagccgctg ggcgacgccg aaactggcgg
     5701 tgaccgccag gttctcgtgg ccgagaggaa aacgcagctc ggcgaggcgg ccgcggacgc
     5761 gatcggccca tttgagcgcc tgctgctcgg tggtctcggg caatacgatg cagaattcct
     5821 cgccgccgta gcgcccggcg atgtcgctgg cgcgaacgct ctcggaaagc acgcggccga
     5881 actggcggat gacctcgtcg cccgccgggt ggccgtgcag gtcgttgatc cgcttgaaga
     5941 agtcgatgtc gagcatcacg caggccagcg gcgcgttgta gcgctgcgcc cgacgccatt
     6001 cgcggcccag gtcgtcgaac agcgtgcgcc ggttcgccag gccggtgatc gcgtcggtgt
     6061 tggcgatggc gctcaggcgg tgctcgagct cggcgaaccg cgctccggcc cgcaagcggg
     6121 cggtcagctc attgcgatcg acgggcttct tgagaaagtc gtccgcgccg gcctccaagc
     6181 cgctgagcat gtccgctgag tcgccgcggg cggtcaggaa gatggtgtag atgtagtggc
     6241 cgaaatccag cccgcgaacc cagcgcatca actccacacc gtccagctcg ggcacgtccc
     6301 agtcggggat caagatcgaa ggccgcgcca gctcgatcag ctctcgcgcc tggcgtccat
     6361 cgtgggcgca atggacctca tagccgtcag actccagcca tttgcccaaa agccggagca
     6421 tcgccgggtc gtcatcgacg agcaggattt ttggtttttc cggaggcata gggaagtcgg
     6481 gcgaagagtc gtctcccaag aagggtacgt cgggaaacca tcccaggcga ttgcagtaag
     6541 tcgtgttgtg gaaagactct acgacgagcc gtcgggctgg gaacagcgcc actcgaatca
     6601 ccaagcgggc cacccaaacg tacggtgcca gacccaaccc attgacagaa cggtaccatc
     6661 ggctagaaca atcacaactg agactcagta tcaactgcga atgccagccg ccgcaatcat
     6721 gcagcacgcc agctttcttc gccgtggacc gcttatgtcc ccgtggccgc cgaccttcga
     6781 gtctcgccgg cagccgcacg ttgcgaggag ggttcgacca tggtgcgctt ggctcggcgc
     6841 ggatttacgc tcgtggaatt gctggtagtc atcgcaatca tcgggatgct cgtcgcgctg
     6901 ctgttgcccg cggtgcagtc ggctcgcgaa gccgcccggc ggatgcaatg ctcgaacaat
     6961 ctgaaacaga tcacgctcgc ggttcacaac tatcacgacg cgctgctgta tctgccgccg
     7021 ggctcgacca atcctgatct gagcggcagc agcggcctgg ccgcgatcct gccttacctg
     7081 gaacaggcaa gcgcgtactc gctgtacgat ttctcgaaag gcaactccga tcccgcgaac
     7141 ctgcgatcag tctcgcagaa gatcaagacc tatctctgtc cctcgtgtgt gtttgctcgc
     7201 tcggtgccga tcggcggctg cgacgccaac gaccgggctc cgggcactta tgctttttcg
     7261 acgaataccg gcgaccccta ttccctgagc aatggcgcga tcgcgaacgc gatttcgccg
     7321 caaacccgcc tcgcgtcgat caccgacggg acctcgaaca cgttcctcgc cggcgaagcc
     7381 cactgggcgt ttaaggacta tttgttctcg agcggcccgt gcgccgggca agtccgcggc
     7441 ggcttctcgt attggtcttc gccttatccg ctggccaccg tcttcacgac gcgcggccct
     7501 ttcaacgccc agtcgatgag cggcgacagc ctgcggctgg ccagcttccg cagcagtcac
     7561 gcggcgggcg tcaacatgac gaacgtcgat ggctcggtac gcttcgtgca gcagtcgatc
     7621 gatcacaccc tgctcgacgc cggcgcaacc cgcgacggcg gcgaggcgct cgcattgccg
     7681 taggccggta gccacagaaa acacgagaag cacgaaaaac caaccaatag aaatccgatc
     7741 ctgtgactca acacattctt gtgagatctc gtgttctttg tggccagttc ctgtgcctgc
     7801 tttttgcggc ggtggtcggc tgcggcgaat cggggcctgc tcgcgcgccg atcaccggcc
     7861 aggtgacggt cggcgggcag ccgctggcga agggccgcat cctgttcatt cccgcggcgc
     7921 ccaaccaggg accggttgcg tcggcggccg tcagggacgg cagctaccgc ataccgcagg
     7981 tcgaaggtcc cgtcgttggg caaaatcgcg tcgaagtcga ggcggagttg aacctcggct
     8041 tcgccatcga cgacgaggcc gccttcgcca agcgcggcgg ccggccgctc cccgccaatc
     8101 cgatcccgcc gcaatacaac cgcgattcca agttgacggt ggaagtcgaa gccggcgacg
     8161 agaaccgctt cgacgttaca attcccagcg cccggcacgc tgccgcgcgc ccgcagtaat
     8221 gaacctcgct tacggtagcc gaattcgcca gaattcggat tcccagctcc atccgaactc
     8281 tggcgagttc ggctacaaac ctctggaggc cgaacatgtc tttgccatct gccttgctca
     8341 cggtcggctt tgtcgccgcg gcctgcttgc tcgccggtgc ggcccacgcc gccgacaacg
     8401 tgcaaatctc cgaattgccg ccgctggaaa aaccggtcac cagcttcggc gcagccgtgg
     8461 ccgacggcta tctctatgcc tttggcggcc acctgggctc gccgcacgag tactcgggcg
     8521 atttgcagtc ccgagagctg ctgcggctga atctgaagca gccgcaaaag tgggagctgg
     8581 tcggcgaagt tccgcggcgg accgggctgg cgatggtgag ttatggaggc aagctctacc
     8641 gcgtcggcgg ctgggaggct cgcaaatcgg ccgaaggcaa gtgggaattg ttctcgacca
     8701 gcgactttgc ccgcttcgat cccaaggatg gcaagtggga agacctcgca ccgctgccgc
     8761 acggccgcag cagccacgac gcggcggtgc tgggcagccg gctatatgtg atcggcggct
     8821 gggaattggg cgataagacc ggcggccagt ggcacgaaac ggcgtatgtc tgcgatctgg
     8881 ccgacgcgcg gccgcagtgg aaggaaatcg ccaagcctcc cttcaatcgc cgagcgctgg
     8941 ccgtcgcggg ctacgcgggc aaggtttacg ccatcggcgg catggacgac agcaacgaag
     9001 tcacgctggg cgtggacatc tacgatccgc agacgaacgc ttggagcaag ggcccggcga
     9061 ttcccggcca gggctttgac ggcttcggct cgtccgcgat cgggacgaag gccggactgt
     9121 tcgtcacgag tgcgtccggc agcctgtttc ggctaaacga cagcggcaag tcctgggacg
     9181 aggcgacgaa gctcaagcac gcgcggacgt ttcatcgcct cgtggcagac ggcgatcagc
     9241 ggctgctggc tgtcggcgga acgtcgaagg gcaccaaggt gccgcaggtc gagttgatcg
     9301 agctgaagta acaacgcctt caatcctcgg agcccttatg acacgacttg catcgttcgt
     9361 cgccgttggc gcgctgctgc tggctgccgc gcctgctcgg gcccactttg tctgggtctc
     9421 ggtcgagaag gactccagcg gcaagcccgc ggcgcacgtc tggttcagcg aattggcgga
     9481 gccggacagc gctgaactgc tggaccgcat cacttcggtc aaggtcacca gtcgcacggc
     9541 cggcggcaag cccgcggcag tcgctgtcgc caaacaagtg caaggcaatg gaggcgcgct
     9601 ggtcggcgcg attcccgccg gcacgccggc tctgtcggcc cacatcaaat acggcgtgat
     9661 cacgcgcggc gagagcacct tcctgctgca atacagcgcc aagtatctgg atgcagccgc
     9721 gcaggatttc aaggcgctgg cccgcgacga gtcgctgccg ctggacgtcg tgccgcaggc
     9781 ggaagccagc ggctacgtgc tggaagtcct ctttcaaggc aagccggccg ccggcgcgga
     9841 gatcgtggtc tacgatccga cggccgccca gctggacctc aagaccgatg acaagggccg
     9901 ctgctcgctg gcggcggcga agccgggcct gtattcgatc cgggccaggt ggatcgtgaa
     9961 ggaagcgggc aaggagcagg acaaggaata tccgcaggtc aactactaca gcacggtgac
    10021 tctccgcgtt ccgtcaaaat aatccacgtg cgaccgcggt ctcgcgctgg ctgccaatct
    10081 ccgcggcggc cgccgaggcc gcggcctttt taccgatcca tgaacgagct gacccaacca
    10141 gaggcgtcag ggcccagcga gccggccgat gccgccaatc ggccggccat gccgcgcgag
    10201 gcctggcgtt ccgaggagtt gctcggcgag cggaccgagg tgctgatcct gcacaatgga
    10261 gccgtctatc gcttgcgccg cacgcggcag gggaagctga tcctctataa gtaggcccct
    10321 tctgccggaa ggggccttgt gggaagcgca gcgaccagga acaccgccga gaatgggacc
    10381 gttgcgtgct gctcaggtcc cgcccggcag gcgggaccta caagtctgga agccgcaaca
    10441 cccgcccgat agactcaagc gctgctcatc ctgctccgct tggcactgaa gggccggcaa
    10501 ggaatcgccg gcagacagtc catgagtcaa ttctggctct ggcgccccgc gaacttcgcc
    10561 gcacccctca cggcccagta ttcggcgttt cgcggcgagc ccctggagct gacgctgatc
    10621 tttgccgatc tggcgacgtt cgcggccttt gccgtgatcc cgctgatgat cctctatttc
    10681 ctgctgcggc ggcggcaggt gcacttctcg cgggtctggt tcctcctgct catgtacctg
    10741 atcgtgggcg gggccgtgca cgtgctcgac gcctgcggca actgggagcc ggtagtgcac
    10801 ctggcggctg cgctgaaggt cgtggtggcg ttcatcgcct ggatctgggt gatcgtgttg
    10861 attccgctgt tgcccaagct gctcgaagca cggagcgcgg aggaattcaa gacgctgctc
    10921 tcgaagcacg aagaggcaga gcaggcgcta aaagaaacgg aagcggtcta caagtcgctg
    10981 atcgagagcc tgccgctgaa cgtgtttcgc aaggatctgc agggctgctt cgtcgatgcc
    11041 aatcagcggt tctgcgacac gctaggccag ccgctggcgg cgatcgtggg caagtgcgac
    11101 gcggactttt ttccggcgga gcaggtgcag aagtaccgcc gcgacgacac caaggtcgcg
    11161 gagtccggcg aagtgctgga agacatcgag ttctatttca agcccagcgg cgaaaagctc
    11221 tacgtgcagg tgctcaaggc cccggtccgc gacgccagcg gccagatcgt cggcgtgcag
    11281 ggaatgttct gggacgtcac cgagcggatg cgggccgacg aagcggcccg gctctccgac
    11341 gcccgcttcc gcaagctcgt gcagtcgaat ttgatcggcg tgctcgtggc ccgcctcgac
    11401 ggcagcgtcg tcgatgccaa cgacgcgctc ctgcagcggc tgggctacac ccgcgacgac
    11461 ctggaggccg gccgcttccg ctgggacgcc ctgactcccc ctgaacaccg ccctgccgac
    11521 gagcgggcaa tcgagcagct ccgggcaacc ggagtttgtc agccgtggga aaaggaatac
    11581 ttccacaagg acgggcatcg cgtgccgatt ctgatcggtg tgacgatgct cgaaggttcg
    11641 gagacggagt gcatctgctt catcgcggac atcacgaatc agaagcagac cgagcgcgag
    11701 ctgaaggatg ccaaacaggc ggccgacgca gccaatcagg ccaagagcca gttcctggcc
    11761 aacatgagcc acgaggtccg cacgccgatg aacgcgatca tcggcatcac cgagctggtg
    11821 ctgaagacgc cgctggcccc caagcaggcc gagtacctgc ggatggtgat gcagtcggcc
    11881 gagtcgctgc tgggcgtgat caacgacgtg ctcgattttt cgaaagtcga gtccggcaag
    11941 gtcgagctgg agcagcagcc gttcagcctc cgcgagagca tcggcgacgc ggtgaagtcg
    12001 ctggccttgc ggacgcacga cagggggctg gagctggcgc tcgacgtggc gcaagacgtt
    12061 cccgactggc tcgtgggcga tgccggccgc ctgcggcaga tcgtgatcaa cctggtggga
    12121 aatgcgatca agttcacgca ccagggggag gtagtggtgg aggtcaggag ccaggagtcg
    12181 ggagtcggga gtcgggagtc ggcggggaat cctccgaccc ccgactcccg atccccgacc
    12241 cccgactcct gccacctgca gttcctcgtc cgcgataccg gcatcggcat tccgcccgac
    12301 aagctcgaga aagtcttcga ggccttcgag caggccgatg cttccacgac gcggcactac
    12361 ggcggcacgg ggttgggcct ggcgatcgtc cgcaagctga tcgagctgat ggggggccgc
    12421 atctgggtcg agagcaaagt cggcgagggg acgcggttct atttcaccat tcgcctccag
    12481 cgctgcctgc tgccgccgcc ggcgaggccc gagcccaagc ggggcgcgct gaagggcacg
    12541 cgggccctgg tcgtggacga caacgtaacc aaccgccgga tcattgaaga ggcgctgcac
    12601 agctgggagg tggttccctc ctcctgcggc tccgcggctg aggccctggc ggcgctgcga
    12661 gccgcctatc gcagcggcgc gcccttcgag ctgttgctca gcgacgtcaa catgcccggc
    12721 gccgacggct tcgccctgct ggagcaaatc cgccgcgacc cgagcctggc cgacatcacg
    12781 gcgattctgc tcacttccag cgagcgggcc gaagacgccg cccgctgcca gcagctgcgg
    12841 gtcaaccagc ggctgaacaa gccgatcaag caatcggagc tgcaaggcgc gatcctcgaa
    12901 gcactcggca tcgtcgaccg ccccgccgtg ccggacgacg ccgacgccgc ggctctgccc
    12961 accacgcggc cgctgcgaat cctgctggcc gaggacagcc tggtcaacca gcggctggcg
    13021 ctcgggctgc tggagcggca cggccaccgc gtcgcgatcg ccaacaacgg ccgcgaggcc
    13081 ctggagcagt cggccgccga cagcttcgac gtgatcctga tggacgtgca gatgcccgag
    13141 ctggacggcc tggaagcgac ccgcctgatc cgcgagcggg agcgcggcag ccagcggcac
    13201 gtgccgatcg tggcgatgac cgcgcacgca ctcaaagggg accgggaacg gtgcctggcg
    13261 gccggaatgg atgaatacgt ctccaagccc gttcgcgagc ggcagctcct gaccgcgctg
    13321 cggacggtgc tgggccagga agtcgccgcg ctgcccgacg agctggagcc gcagctggcg
    13381 ccagcgacgg acatcttgaa ttgggacgcg gccctcaaga tctgcggcgg cgaccggaat
    13441 ctattgcgtg aaatcgtcct ggccttcctg gaggaacagc cccaacggct ggatgaaatc
    13501 cgccgggcga tcgacacgtc cgactgggag ctgttgcacc gtgcggccca caccatcaag
    13561 gggtcgatgc ggtatttcga ggctcgggcc gtgtttgacc gggctttcgg cttggaacag
    13621 ctggccgccg cccggcggct ggacggggcc gaagagattt tcacccttct caagcaggag
    13681 ttggcaaagc tggtccccca tctgataagc tacgtgcaag gtcgtggcgg accaaccccg
    13741 gccagccggg gctagaacca tccttgtcga atttggagcc gcgccatgcc taccgtcttg
    13801 gtagtggacg attcggccgt cgatcggcgt tttgtgggtg gactgctctc gcgcgacggg
    13861 aggtttcaag tcgagtttgc cgaggacggc gggcaggctt tgggccgcat gcgccaatcg
    13921 ccccccgacg tgatcgtgac cgacctgcaa atgcccaacc gcaacgggct cgaattggtc
    13981 gcggccgtgc ggatgcatca tcccgaggtg ccgatcatcc tgatgaccgg ccacggcagc
    14041 gaggacctgg ccgtcgaagc gctgcaccgc ggcgcggcca actacgtccc caagccgcag
    14101 ctcggcgagc ggctgatgga cgcggtggaa gaggccctct cgatggcccg ggccgaccgg
    14161 acctacgacc agctcatcgc ctgcatggaa aagtgcgagt tcgccttcga gttcgaaaac
    14221 gaccccggca tgatcgatcc actggtcgat ctgattcagc agatggtcgc gggcatgaag
    14281 ctgaccgacg ccaccggccg ctaccgcgtg ggggcagccg tcaaggagtc gctgctcaac
    14341 gcgatctacc gcggcaacct ggaaatcagc ttcgaccaga tgcaagacac ccgcgtctcg
    14401 ctggtggagg gcaagggaga ggacctgctc cagcagcgca gcagccaggc cccgtacaaa
    14461 gatcgcagag ttcaggtaag cgtctttctc aatcccaaag aagcccgctt tgtcatccgc
    14521 gacgccggca aaggattcga tccggccaag gtccccgctg ccggccggcc cggatcgctc
    14581 gacccgcaat cgggccgggg gctggtcttg atgcgggcct tcatggacga agttacgttc
    14641 aatgccgcgg gcaacgaagt cacgctgctc aagcggcgcg agtaggcgca ttcgggcggg
    14701 tcgaattatc attcgaaggg atagagatct cctgtcgatg gcaacggttc tcgtagtcga
    14761 tgacgccgcg gtcgatcgca agctcgtcgg cgggctgctg gggcgggggc ccaacctgac
    14821 ggtcgagttc gccgcgtcgg gcgacgaggc cgtcgagcgt ctggcgaccg tgcagccgca
    14881 gattatcgtc accgacctgg tcatgccggg catgtccggc ctggatctgg tcgctagaat
    14941 cgtcgcagat tacccacaga ttcccgtcat cctgatgacc ggcaagggca gcgaggagat
    15001 cgccgtccgg gccctgaagg ctggcgccgc cagttacgtt ccgaagtccg cgctccacca
    15061 actcctgctg gccaccgtag aggacgtgct cgatatggtt cgacaaaggc atacgcaaga
    15121 acgcctgatg ggctgcctga aacaggggga gtttcagttc gccttgggca acgatgccag
    15181 cctgatcccg tccctcatca attacgtaca gagcctggtg tgcagcgtcg gcctgtgtgg
    15241 cgaagccgac gtgattcgcg tctgcatcgc cctcgaggag gccctccgca atgcgatgta
    15301 ccacggcaac ctggagctga ccagcgagca gcgggaaggg gacccggtga agtaccagga
    15361 gctgatcgaa gaccgcaaca cccgcgaccc gttcgcgggg agggtgctga cggtcaccgt
    15421 gagcgtcacc ccgaccagcg ggcggtttac catccgcgat caagggccgg gattcgatcc
    15481 gcggaagctg cccgatccga ccgacccgga aaacatagaa aaagtcagcg gccgcgggct
    15541 actactaatg cggacgttca tggacgaggt cgtgttcaac gagtcgggca accaggtcac
    15601 gatgatcaag cgcctgccgg acacgccgca gtgaccagcg cgggaagagg ggagcgcggg
    15661 aagaggggag cagagccaac cggggaacga ggaccgaatt cgccagcatt cggaagttca
    15721 tgaccgcggc cggactctgg cgagttcggc gaccatctgc tgagaagcgc cagtgagcga
    15781 ttcgaaatac gtccgcacgg agcaattggg cgacgccctc gtcgtcacgc ccctgttcac
    15841 cttcgccgcc ttcgtcgagc cggatctggc caacgaatgg tccgaggtgc aaaagcaggt
    15901 cgaggcgccg ggcgtcaaga acgtagtcgt cgatctcagc gcgatcccct atttcggctc
    15961 gaccgtcctg gagtggatgg tgcagatgtg gaagcgggcc aaggccaagg ggggcaaact
    16021 cgccacctgc caggcctcgc ccatcggccg cgaagtgctg atcaccgccc gcttccatca
    16081 gctctggggc atctgcgatt cgcgggccga agccctggcc tgggttaccg ccaaatagtt
    16141 gcttagaaac aacttgagcc gcggggcgtc ttgtcccgcg gctcaagtgc tttataagac
    16201 cttcggcgga ttgcgacgtg tggagtcgct gggagagtgg cgaggtctgg cgtgctactg
    16261 cacgtgccgt tggtgaccca ggccatcccg gaatagttgg tccagcagtc ggaatatctg
    16321 ccgaaggttg tctcgatcga ttgtccgccg ccgcttccgt gccgccgcgg gcaaaaatgt
    16381 ttatcggttg ctccgccgtc ggcgattgag cgaaaccgcc cgcagcgtat ggctagttta
    16441 gcggctgaat cgactcggtg aagcttgcag ccgccaactt tgcccgccgc gcccgccacc
    16501 gaggcgtgtt actcatggcc ccgccacgct ttgcggcgac cgtgaatttt gccgtaattt
    16561 tccgctgact acagctccgc gcccaactgc cgcagaaact cgaacgaaaa gatgcctgtg
    16621 tcgtgcccgt cgctgaacgc aatcgcgtag gcatagttcc ccatcggctg catgccggca
    16681 atcgccagcg gctgggccag ggcgggcgac agcgtcggca gcgccagggg gtcggccggc
    16741 tggctgcgct tctcgcggca ggtcgcgcag gggcaagcgt cgcgcagctg ccgcggcgta
    16801 tagcgccgcc tctgcccatc gctccaagtg atgagcaggc tatttccctc gcgggccagc
    16861 tcggtcggat agtgcatgga cgccgattcg tttaacccgg agccggaggc ggcggctact
    16921 ggaaggtgct gtggtcgccg gcacacgcgg ggcattatag tagagaggtc aaaccgggcc
    16981 gacgaaaggc cgcactcctc ttgggcgtag cgacgcgtac gtgttcagct tcctcgtcat
    17041 gacgagcggc ccccgcgccg gggcccagtt catcctcgat cccagtcgcc agaaccgcat
    17101 cggccgcggc ctggattgcg acgtgatcct gtccgacccg ctctcctcgc gggtccacgc
    17161 gatcatcgtc tccagcgacg gcgactggtg ggtccgcgac gccggcagcc gcaacggcac
    17221 ctacgtcagc agccagaaga tcgacgaggc ccggctggtg gacggcggcg tcctgaaggt
    17281 gggctcgacg gagttcgagt tccgccagac gctggcccgg ccgaccgacg ccacgcggcc
    17341 cgaccacacg cagacgatca tcagcgaccg cagcgtcctg ctcggccagc aggcgggcga
    17401 gttcgggctg gaggccctcc gcgacaacgt gcgggcccac gactttctc
//
LOCUS       DASRJB010000378         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_105815, whole genome shotgun sequence.
ACCESSION   DASRJB010000378 DASRJB010000000
VERSION     DASRJB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105815"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFB80_09975"
     CDS             complement(<1..270)
                     /locus_tag="VFB80_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZN34138.1"
                     /translation="MFVAKVTGSLVSTQKVEAIKGYKLLVVEPYRVDPAQRKSLVTTG
                     RTFVAVDMLGAGVGDFVLLTQGSSARMTPETKNMPIDAVVIGIVDQ"
     gene            complement(347..1534)
                     /locus_tag="VFB80_09980"
     CDS             complement(347..1534)
                     /locus_tag="VFB80_09980"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZN34139.1"
                     /translation="MKILVANLGSTSFKYRLFDMADERCLARGGVERIGAAESPCFVE
                     IGGKKTALTAKVPDHAAAVRQCLAQLTDPRTGCLASANEVAAIGFKAVHGGRISGVQR
                     VTPDVLAAMEQMSLVAPAHNPPYIKAMRLLAEKLPEIPLVAAFETGFHATIDDRLRYY
                     AAPYEWAEKLHIKRWGFHGASHRYIAQRTSQLLGRYDLRIVSCHLGGSNSLCAIRGQQ
                     SVATTLGMSPQTGLPHNNRVGDFDPFAIPLIMQAEGRSLHQVLDTLAEKSGLLGLSGI
                     SGDIRDLEEAAAQGKARAKLALDVFVAEIRRHLGGMLIELGGVDVIVFTGGIGENGVG
                     IRAGVCAGLEELGISLNAALNAKAKGESRISGENSRVQIWTIPTNEELIVARQTRHAL
                     ENA"
     gene            complement(1619..1885)
                     /locus_tag="VFB80_09985"
     CDS             complement(1619..1885)
                     /locus_tag="VFB80_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZN34140.1"
                     /translation="MSAIGLIETKGLVGLVEATDAMAKAANVQILKRIGIGGAYVTTI
                     VTGDVGSVRAAVEAGAQAAQQVGELVGSHVIPRPAEGLLEAFVG"
     gene            complement(1978..2277)
                     /locus_tag="VFB80_09990"
     CDS             complement(1978..2277)
                     /locus_tag="VFB80_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZN34141.1"
                     /translation="MAKVNEALGMIETKGFIALLEATDAMLKAANVQLVGWDKVGSGL
                     VSAFVTGDVAAVKSATDAGAAAAGRIGEVVSVQVIPRPHDDLHIVLPPTLKKAKG"
     gene            complement(2376..3086)
                     /locus_tag="VFB80_09995"
     CDS             complement(2376..3086)
                     /locus_tag="VFB80_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HZN34142.1"
                     /translation="MSAPTQLDRSLVESIVRQVVLAQTAAKPAPKAAEPLKPDLVVSI
                     SARHCHLTDEHVEVLFGPGAKLIPEKPLYQDGFYAAEQTVMIVGPRRRMLPTVRVLGP
                     TRPHSQVELAFTDSISLGIDAPVRHSGNIKGTPGCVLVGPKGVVELPEGVIRAARHVH
                     MNFDHAAYYGVKNGDLMRLRVESPQCAVVFEDLLVRADKNAKLEVHIDTDEGNACYLD
                     SATKVELTKQDGGCGCKH"
     gene            complement(3083..3889)
                     /locus_tag="VFB80_10000"
     CDS             complement(3083..3889)
                     /locus_tag="VFB80_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HZN34143.1"
                     /translation="MIKASLADDRRSRVLELIRLRGFASLPALAEELAVSESTIRRDL
                     DFLEESGVAQRTHGGVFYTGPSPKLAHFDQRQALNWDRKRQIAAAASRLIDDNDTILL
                     DGGSTTYELAQLLVGRPLQVVTNSLPVANLFTSSGNADLVFVGGYVHAKTGVSIGPYA
                     NQMLAGLNVRRAVLSVAGINERGTFNSNLLLVETERAMIASAEQVIVVADSTKFGHTS
                     LAQMCPLQEIDVLVTDSEIGAEWPPRLEAAGVKLVVAEAEKNGRPEARPT"
     gene            complement(4068..4661)
                     /gene="purN"
                     /locus_tag="VFB80_10005"
     CDS             complement(4068..4661)
                     /gene="purN"
                     /locus_tag="VFB80_10005"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZN34144.1"
                     /translation="MTPPLSIAVLISGGGTTLRNLIEKIAAGKLPVEIRLVISSNPAA
                     GGLKFAAEAGIRSLVVEKSKSAGADQFSAEIFGACRQAGVRYVVMGGFLKHVLIPPDF
                     ENRVINIHPALIPAFCGKGMYGLHVHESVLAYGAKITGCTVHFVDNQYDHGPILLQRA
                     VEVLPADTPLLLQARVFAAECEALPAALRLLAESPSR"
     gene            4700..>5903
                     /locus_tag="VFB80_10010"
     CDS             4700..>5903
                     /locus_tag="VFB80_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34145.1"
                     /translation="MLPLPSRREFLAASALTVASSQRLAAGQEPPAASGGAFPVKIAV
                     DAAKPQRELKPIWRFFGCDEPNYAYLKHGRKLVGELGELSPKNVFFRTHNLLCSGDGT
                     PALKWGSTGAYREDASGKAAYDWTILDRIFDAYLERGVRPYVQIGFMPKELSTRPEPY
                     QHTWQPGRRYDDIYTGWAYPPKDYARWGEIAFEWTKHCIARWGRAEVETWYWQVWNEP
                     NIGYWKGTPEEFRKLHDFAIDGVRRALPTAKVGGADTAGSGGRFTREFLDHCQRGTNH
                     ATGKTGTPLDFVSFHAKGAPSVVDGRVRMGIANQLRTIGDGFRIVASYPELKDKPIVI
                     GESDPDGCAACSARIYPSNGYRNGPLYASYTAASFARKHDLAEQYGVNLEGALTWAFE
                     FEDQPLFAG"
BASE COUNT          991 a         1928 c         1893 g         1091 t
ORIGIN      
        1 ctgatcgacg atgccgataa cgacagcgtc gatcggcatg tttttggttt cgggcgtcat
       61 ccgggcgctc gagccttggg tgagcagcac aaaatcgccg acgcccgcgc cgagcatatc
      121 gacggcgacg aacgtgcggc cggtcgtcac gaggctcttc cgctgcgccg gatcgacgcg
      181 gtacggctcc acgaccagca gcttgtagcc cttgatcgcc tcgaccttct gcgtcgaaac
      241 gagcgagccg gtgactttgg cgacgaacat ttttgtcagt tgtcagtggt cttggtcagt
      301 tgagtgggct gtgcccacca cggggcgccg gcgggcacag cccaccctac gcattctcca
      361 gagcgtgtct cgtttggcgg gccacaatca gctcttcatt cgtcgggatc gtccaaatct
      421 gcacgcggct gttctcgccg ctgatgcggg attcgccttt ggccttggcg ttgagggcgg
      481 cgttcagcga aatgccgagc tcttcgagcc ccgcacaaac gccggcgcgg atgccgacgc
      541 cgttctcgcc gatgccgccg gtgaagacga tcacgtccac gccgccaagc tcgatgagca
      601 tgccgcccag gtgccggcgg atttccgcca cgaagacatc gagggcgagc tttgcccgcg
      661 ccttgccttg agccgcggct tcttccaggt cgcggatgtc gccgctgatg cccgacaggc
      721 ccagcagtcc cgatttctcg gccaacgtgt cgagcacctg gtgcagcgaa cggccttcgg
      781 cctgcatgat cagcgggatc gcgaacgggt cgaagtcgcc gacgcggttg ttgtgcggca
      841 agccggtctg cgggctcatg cccagcgtgg tggcgacgct ttgctgcccg cgaatcgcgc
      901 acaggctgtt gctgccgccc aggtggcacg aaacgattcg gaggtcgtac cgccccagca
      961 gctggctggt ccgctgcgcg atgtagcggt ggctcgcccc gtgaaagccc cagcgcttga
     1021 tgtgcagctt ctccgcccat tcgtacggcg cggcgtagta tcgcagccgg tcgtcgatcg
     1081 tggcgtgaaa gccggtctcg aaggcggcga ccagcggaat ttccggcagc ttctcggcca
     1141 acagccgcat cgccttgatg tagggcgggt tgtgggccgg ggcaacgagg ctcatctgtt
     1201 ccatcgccgc cagcacgtcg ggcgtgaccc gctgcacgcc gctgatgcgt ccgccgtgca
     1261 cggccttgaa gccgatcgcg gccacttcgt ttgcacttgc caggcagccg gtccgtgggt
     1321 cggtgagctg cgccaggcac tggcgaacgg cggcggcatg atcggggacc ttggcagtca
     1381 gcgccgtttt tttcccgccg atctcgacga aacaggggct ctccgccgcg ccaatccgct
     1441 cgacgcctcc gcgcgcgagg cagcgttcgt cggccatgtc aaacagccga tacttgaagc
     1501 tggtcgagcc gaggttggca acgaggatct tcatctggcg agcggtgggc gtgagcccac
     1561 cgatggtccc gaccgatggc gtggcaaagc aatcgggagg cttacgcctc ccgctcgcct
     1621 aacctacgaa cgcttccagc aggccttccg ccggccgcgg gatcacatgg ctgccgacaa
     1681 gctcgcccac ttgctgggcg gcctgcgcgc cggcttcgac cgcggcgcgg acgctgccga
     1741 cgtcgccggt aacgattgtc gtgacgtagg cgccgccgat gccgatccgc ttaagaatct
     1801 gcacgttggc ggctttcgcc atcgcgtcgg tcgcttcgac cagcccgacc aggcccttgg
     1861 tttcaatcag tccgatagca ctcatggata gaaccttgtg gttgtgggtc gtggggcagg
     1921 cattcttgtc tgccgtttct gttcttgcgg cagacaggaa tgtctgcccc actgggttta
     1981 gcctttcgcc ttcttcagcg tcgggggcag cacgatgtgc aggtcgtcgt gcgggcgggg
     2041 gatcacctgc acgctgacga cttcgccgat ccgccccgcg gctgccgcgc cggcgtcggt
     2101 ggcgctcttc acggcggcga cgtcgccggt gacgaaggcc gagacgaggc cgctgccgac
     2161 cttgtcccag ccgaccagct ggacgttcgc cgccttgagc atcgcgtcgg tcgcctccaa
     2221 gagggcgata aagcccttgg tttcgatcat gccgagcgct tcgttgactt tggccatgtg
     2281 ggtctccggg gtttagtggc tagtgctggt gctagtggct agtgccaggg gctggttcgg
     2341 aatcactagc caccagccac tagcactagt cactcctaat gcttacaccc gcagccgccg
     2401 tcttgcttcg tcagttcgac tttggttgcg gaatcgaggt aacaggcatt gccttcgtcg
     2461 gtgtcgatgt ggacttcgag cttggcgttt ttgtcggccc gcacgagtag gtcctcaaag
     2521 acgaccgcac actgcggcga ctcgacgcgc agacgcatca agtcgccgtt tttcacgccg
     2581 tagtacgcgg cgtgatcgaa gttcatgtga acgtggcggg cggcgcggat gacgccttct
     2641 ggcagctcga cgacgccctt ggggccgacg agcacgcagc cgggcgtgcc cttgatgttg
     2701 ccgctgtggc gcaccggggc gtcgatgccc agcgaaatcg agtcggtgaa ggccagctcg
     2761 acctggctgt gcggccgcgt ggggccgagc acgcgaaccg tcggcagcat ccgccgccgc
     2821 gggccgacga tcatcaccgt ctgctccgcc gcgtaaaagc cgtcttgata gagcggcttt
     2881 tctggaatca gcttcgcgcc ggggccaaac agcacttcaa cgtgctcatc ggtcagatgg
     2941 cagtgccggg ccgagatgct cacgacgagg tccggcttaa gcggctccgc ggccttgggc
     3001 gcaggcttcg ccgccgtctg cgccaggacg acctggcgga cgatgctctc gacgagcgag
     3061 cggtcgagtt gggttggtgc gctcatgtgg ggcgggcctc tggccggcca ttcttttctg
     3121 cctcggcaac caccagcttc actccggcag cctccagccg cggcggccat tccgcgccga
     3181 tctcgctgtc ggtcaccagc acgtcgatct cttgcagcgg gcacatctgg gccaggctgg
     3241 tgtggccaaa tttcgtactg tccgcgacga caatcacttg ctctgcgctg gcgatcatcg
     3301 cccgctcggt ttcgaccagc agcaggttgc tgttgaacgt cccccgctcg ttgatccccg
     3361 cgacgctcag caccgcccgc cgcacgttga gcccagcgag catctggttg gcgtacggcc
     3421 cgatcgacac acccgttttg gcatgcacgt agccgccgac gaacaccagg tccgcgttgc
     3481 cgctcgacgt gaacaggttc gcgacgggca gcgagttggt caccacttgc agcggccgcc
     3541 cgacgagcaa ctgtgccagc tcgtacgtcg tgctcccgcc gtccagcagg atcgtgtcgt
     3601 tgtcgtcgat gagccggctg gcggcggctg cgatctgccg cttcctgtcc cagttgagag
     3661 cttgccgctg atcgaagtgc gccagcttgg gcgacggacc ggtgtaaaac acgccgccgt
     3721 gcgtccgctg ggccaccccc gactcttcga ggaaatctag gtcacggcga atcgtcgatt
     3781 ccgaaaccgc cagctcttcc gccaatgccg gcagcgacgc aaacccgcgc agccggatta
     3841 gctccagcac gcgcgaacga cggtcgtctg ccagcgaagc cttgatcaca gccaatacac
     3901 tccacgcaac ttgccccaat tatgacgctg gctgtgaaga aatcaatcag cttgtgatag
     3961 aattctgtca aatatgggcc gttaccggtc gaacaattcg aatgcgatga tcctaagttg
     4021 tttctgtaca aagcgttgcg actagcgaat agctatcgct cggcagatca ccgagatgga
     4081 ctctcggcca gcagccggag ggcagcgggc agggcttcgc actcggcggc gaagacgcgg
     4141 gcctggagca acagcggcgt gtctgcgggc aaaacttcga ccgcccgctg caaaagaatg
     4201 ggcccgtgat cgtactggtt atccacaaag tggaccgtgc agccggtgat cttggcccca
     4261 taggcgagca cggattcatg cacgtgcaga ccatacatcc ctttgccgca gaaggccgga
     4321 atcagggctg ggtggatgtt gatcacgcgg ttttcgaagt ctggcggaat cagcacgtgc
     4381 ttgaggaagc cgcccatcac gacgtaccgc acgccggcct ggcggcaggc gccgaagatc
     4441 tccgcgctga attgatctgc gcccgcggac ttcgattttt cgacgacgag cgagcggatg
     4501 ccagcctcgg cggcaaactt cagtccgccg gccgccgggt tgctggagat cacgaggcga
     4561 atctccacgg ggagcttgcc cgcggcgatt ttctcaatca aattgcgcag cgtcgtaccg
     4621 ccgccggaaa tgagaacggc gatggagagc ggcggagtca cggaatcatt ctagacgact
     4681 ttgcctcgac tgctcagcca tgttgcctct accttcccgc cgggagttct tggccgcgtc
     4741 ggccttgacg gttgcctcgt cgcagcgcct ggccgctggg caagagccgc ctgccgcatc
     4801 cggcggagcg tttcccgtca agatcgccgt cgatgcagcc aaaccgcagc gcgagctgaa
     4861 gccgatctgg cggttcttcg gctgcgacga gcccaactac gcttacctga aacacggccg
     4921 caagctggtc ggcgaattgg gcgagctctc gcccaagaac gtcttctttc gcacgcacaa
     4981 cctgctctgc tcgggcgacg gcacgccggc gctgaagtgg ggctcgaccg gagcgtaccg
     5041 cgaggacgcc agcggcaagg cagcctacga ctggacgatc ctcgaccgca tcttcgatgc
     5101 ctacctggag cgcggcgtgc gcccgtacgt gcagatcggc ttcatgccca aggaactctc
     5161 gacgcgacca gagccctatc agcacacctg gcagcccggc cgccgctacg acgacatcta
     5221 taccggctgg gcgtacccgc ccaaggacta tgcccgctgg ggcgaaatcg ccttcgagtg
     5281 gacgaagcac tgcatcgcgc ggtggggccg ggcggaggtt gagacctggt actggcaagt
     5341 ctggaacgag cccaacatcg gctactggaa agggacgccc gaggagttcc gcaagctgca
     5401 cgactttgcc atcgacggtg tgcgacgggc cctgccgacg gccaaggtcg gcggagccga
     5461 taccgccggc agcggcgggc ggttcactcg ggaattcctt gaccactgcc agcgcggcac
     5521 caaccacgcg accggcaaaa cgggcacgcc gctcgatttc gtctcgtttc atgccaaggg
     5581 cgcgccgagc gtggtggacg ggcgcgtgcg gatgggcatc gccaaccagt tgcggacgat
     5641 cggcgacggc ttccgcatcg tcgcgtctta tcccgagctg aaagacaagc cgatcgtcat
     5701 cggcgaatcc gaccccgacg gctgcgcggc ctgctcggcg cgtatttatc cgtcgaacgg
     5761 ctaccgcaac ggcccactct acgccagcta cacggcggcc agcttcgccc gcaagcacga
     5821 cctggccgag cagtacggcg tcaacctgga aggcgcgctc acttgggcgt tcgaattcga
     5881 agatcagccc ctgttcgcgg gct
//
LOCUS       DASRJB010000379         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2363037, whole genome shotgun sequence.
ACCESSION   DASRJB010000379 DASRJB010000000
VERSION     DASRJB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363037"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFB80_10015"
     CDS             <1..883
                     /locus_tag="VFB80_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34146.1"
                     /translation="ENAELPGFITINPITRVGGAVNFGSSFLPAPFQGTPIGGEGQSL
                     ANAAIPNIANRSLTREQQRKQLDLLQSLNRDRLAEDKVNSELEGVIESYELAFRMQTA
                     VPKIMDFKDESPSTLENYGIGGGVSDGFGRQCLMARRFAEAGVRFIEVGHGGWDQHGN
                     LRTALTRNCTAIDKPIAALIADLKQRDMLKDTLVVWGGEFGRTPHAQNTDGRDHNATG
                     YSMFVAGGGVKGGLRYGATDEHGIRAETDKMHVHDLHATVLHLLGLDHEKLTYRYSGR
                     DFRLTDVHGVVAKDILA"
     gene            1027..2235
                     /locus_tag="VFB80_10020"
     CDS             1027..2235
                     /locus_tag="VFB80_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZN34147.1"
                     /translation="MFLLCASVRAEITVHTDFEGASAKVLALDQKSRTLRIMPAGDPA
                     HGWPCWWYLRVNGLAAGQTLTLEVAGSDAKLVREGKNFGKPLSASWAQPERATYSSDG
                     KRWRHTEPGLTQDGRRLYSIKADGPSLWLAWGPPFTPQDADELVRSLARERGAEAFEL
                     TKSRGGRPCPALRLKQGDRDEQDRPAIWLQARQHAWESGASWVCRGAAEWLAGDERRA
                     ERLRERCEIYIVPIMDIDSTATGNGGKEQIPQDHNRDWTDMPHHAEVAAAQKHLLRLA
                     GQKRLALFVDLHNPGPGDKQPFFYFCPEETLDERCQKNLDRFFTLCRGEMTGPLELSS
                     RPRVSGASYDPLWKQMSKNWVAAHSPHTVAVTLETAWNTPHSTTDGYRRLGGQLASAI
                     ERYVKDDSRR"
     gene            complement(2248..2973)
                     /locus_tag="VFB80_10025"
     CDS             complement(2248..2973)
                     /locus_tag="VFB80_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN34148.1"
                     /translation="MSRAVSIDPKAAPGLEIPPLVAGDHLTRAEFERRYRAMPHLKKA
                     ELIEGIVYMPSPVSHDDHGAPHFDLITWAGIYRAFTPGVQGGDNSTLHLDLDNEAQPD
                     AHLRILPECGGQSRTVGGYVERAPELVAEVSASSASYDLHEKLKAYRRNGVREYVVWR
                     VWDKSIDWFALRAGQFEPLPPDAAGIFRSECFPGLWLDAAALLRGDLARVVQVVQQGV
                     ATPEHSAFVARLAAARRSGEQPA"
BASE COUNT          553 a         1042 c          975 g          461 t
ORIGIN      
        1 cgagaacgcc gagctgcccg ggttcatcac gatcaacccg atcacccgcg ttggcggcgc
       61 ggtcaatttc ggcagctcgt tcctgccggc gccgttccag ggcacgccga tcggcggcga
      121 ggggcagagc ctggccaatg ccgcgattcc gaacatcgcc aaccgctcgc tcacccgcga
      181 gcagcagcgg aagcagctcg acctgctgca gtcgctcaac cgcgaccgcc tggccgagga
      241 caaggtgaat agcgagctgg agggcgtgat cgagtcgtac gagctggcgt tccgcatgca
      301 gaccgccgtt cccaagatca tggacttcaa ggacgaatcg cccagcacgc tggagaacta
      361 cggcatcggc ggcggcgtga gcgacggctt cggccggcag tgcctgatgg cccgccgctt
      421 cgccgaggcg ggcgtgcggt tcatcgaggt cggccacggc ggctgggacc agcacggcaa
      481 cctgcggacg gccctcactc gcaactgcac ggcgatcgac aagccgatcg cggccctgat
      541 cgccgacctc aagcagcgcg acatgctcaa ggacacgctg gtcgtgtggg gcggcgaatt
      601 cggccgcacg ccgcacgccc agaacaccga cggccgcgac cacaacgcga ccggctattc
      661 gatgttcgtg gccggcggcg gcgtgaaggg cggcctccgc tacggcgcga ccgacgagca
      721 cggcatccgg gccgagaccg acaagatgca cgtgcacgac ctgcacgcga cggtcctgca
      781 cctcctgggc ctggatcacg agaagctgac ctaccgctat tccggccgcg acttccgcct
      841 caccgacgtg cacggcgtcg tcgccaagga cattctggcc tgatgctcaa agccgcgacc
      901 attggtcgcg gttttccagg gttgacgcag ccagcttgtg aaccgcgact actggtcgcg
      961 gctttgggat tgggcatggc gacgcttttc cgaaactttc cgggcgcaag tttgctcccg
     1021 gctgttgtgt ttctgctctg tgccagcgtt cgcgcggaga tcaccgtcca caccgacttc
     1081 gagggcgcca gcgccaaggt tctggccctc gatcagaaat cccgaaccct gcggatcatg
     1141 cccgcgggcg atccggctca cggctggccc tgctggtggt atttgcgagt caatggcctg
     1201 gccgcaggcc agacgctcac gctggaggtc gccggctcgg atgccaagct cgtccgcgaa
     1261 ggcaagaact tcggcaagcc gctgtctgcg agctgggccc agcccgagcg ggcgacgtat
     1321 tcgtccgacg gcaagcgctg gcggcatacc gagcccgggc tgactcaaga tgggcgtcgg
     1381 ctgtactcga tcaaggccga tgggccctcg ctgtggctgg cctggggacc gcccttcacg
     1441 ccgcaggacg ccgacgagct ggttcgctcg ctggcccgcg agcgcggtgc ggaagccttc
     1501 gagctgacaa aatcgcgcgg cgggcgtcct tgtccggcgc tgcggctgaa acaaggcgac
     1561 cgcgacgagc aagaccggcc ggcgatctgg ctgcaagccc gccagcacgc ctgggagtcg
     1621 ggcgcgagct gggtctgccg cggcgcggcc gagtggctgg ccggcgacga acggcgggcc
     1681 gagcggctgc gcgagcggtg cgagatctac attgtcccga tcatggacat cgacagcacc
     1741 gccaccggca acggcggcaa agaacagatc ccgcaggacc acaaccgcga ttggaccgac
     1801 atgccgcacc acgccgaagt cgccgccgcg caaaagcacc tgctgcggct ggccggccag
     1861 aagcggctag cgctgttcgt cgatctgcac aaccccggcc ccggcgacaa acagccattt
     1921 ttctatttct gcccggagga gacgctggac gagcgctgcc agaaaaacct ggaccgcttt
     1981 ttcacgctct gccgcggcga gatgaccggc ccgctggaat tgagcagccg gccacgcgtg
     2041 agcggcgcga gctacgatcc cttgtggaaa cagatgagca agaactgggt cgcggcgcac
     2101 tcgccgcata cggtggccgt cacgctcgaa accgcctgga acacgccgca cagcacaacc
     2161 gacggctacc gccgcctcgg cgggcaactg gccagtgcca ttgagcgcta cgtgaaggac
     2221 gattcgcggc gctaatgcac acccgcttca ggccggctgc tcgccgctgc ggcgggctgc
     2281 tgcgagacgc gccacgaagg cggaatgctc cggtgtggct acgccttgtt gcacgacctg
     2341 aacgacgcga gccaggtcgc cgcgcaatag cgccgcggcg tcgagccaga gaccgggaaa
     2401 gcactcgctc cgaaagatgc ctgcggcgtc cggcggcagc ggttcgaact gcccagcgcg
     2461 caatgcgaac caatcgatcg acttgtccca gactcgccac accacgtact cccgaacacc
     2521 gttacgccgg taggccttca gcttctcgtg caaatcgtaa cttgccgacg acgccgacac
     2581 ttcagccacc agttccggtg cacgctcgac atacccgcca accgtgcgcg actgcccgcc
     2641 gcactccggc aggatccgca ggtgggcatc aggctgcgcc tcgttgtcca ggtccaaatg
     2701 cagcgtggaa ttgtcgccgc cttgaacgcc gggcgtgaag gcccgataga ttcccgccca
     2761 ggtgatgagg tcaaaatgag gggctccgtg gtcgtcgtgc gaaacgggcg acggcatata
     2821 gacgatcccc tcgatcaact ccgccttctt gaggtgcggc atcgctcgat accggcgctc
     2881 gaactctgcg cgcgtgagat gatcgccggc caccagcggc ggaatctcca ggcccggagc
     2941 ggctttcgga tcaatggaaa cagccctact catcgaacct ctccgccaac tgaaatccaa
     3001 ttcccttcca ttctagcccc aatcgcggcc t
//
LOCUS       DASRJB010000380         7007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_390366, whole genome shotgun sequence.
ACCESSION   DASRJB010000380 DASRJB010000000
VERSION     DASRJB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390366"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFB80_10030"
     CDS             <1..909
                     /locus_tag="VFB80_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34149.1"
                     /translation="DASRIARAADERMALVRSYQVGPRGAYTQRLLNELSAARICLLT
                     PASKAQYDAALGQRMRAEPQADAPPRLPPPAPPPTESTALPSAAADDASATPHGWPRL
                     VAAMLAVAALVLCAIGGGIWIRSYWPPARPVEPPRPVVTEPAPPQPIIVLQEGSGEVV
                     LSAATATLAGAVELQVVGTEEVLAHWTTPEDRAVWHFRLVKPGPFYAELTCATSAEAE
                     GFELELVVDDRATAHLLGPSGGFDQFRTDKVPLVIASGGEHTLTLRLAKAPPADWLVL
                     KSVRLVPVSADAPPDAPAEAPAPSAP"
     gene            962..2014
                     /locus_tag="VFB80_10035"
     CDS             962..2014
                     /locus_tag="VFB80_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HZN34150.1"
                     /translation="MRLCFACLVLASASGLSAAEPLLRFDLPGRTVEGTPLAWSEKEI
                     FLLARDGQLIEFPPEQAKNPSKLSDGFKSLSQAEIRGLLLAEFGQGYEVSGVGHYLVV
                     HATGQRDQWAPRFEELYRSFRHYFAARGWSLTEPRFPLVAVVYPRQADFARQAARDGL
                     TQAGGVLGYYSPMTNRILMFDAGSSPNWSNDAETIIHEAAHQSAFNTGVHTRFSATPR
                     WVVEGLGMMFEARGVWQSRTYLHQGDRINRGRLQAYRQRARSRPADAIAQLVSSDRPF
                     SSDPDGAYAEAWALSFFLIESEPKKYQEYLAKTAALAPFAAYPSPQRLKDFTDTFGSD
                     LKLLDARMQRFVAGLK"
     gene            2238..4226
                     /locus_tag="VFB80_10040"
     CDS             2238..4226
                     /locus_tag="VFB80_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34151.1"
                     /translation="MEETLREAPPSDDWRKSLAQTIEQQRHKVGQRIAGQRERLRELE
                     GKIAQHLGEVTQELQSKQDAAREAVAKWQADEASAKQRDADLTRRELEVSKRESDLKA
                     LAAHGSNGSGKEHQHLLEDLTKRLTELDQRHAKVATEEKRLVTRQQELSLRKSELDQL
                     QAGLSGQSAAHQSRADELARQAAELEKARQAHQTEAQRHQQQQSDVQRLQSELKLKAQ
                     SLAEQERETQRQRRHIAQQLRSRKKEQNAEILATGSGQEHELKLKLTELQGKLDRLRE
                     ERDHVQRQLEQAQRKQSELESERQRLQTDLQRHLEEAGRKAEAARLDMARRTSESQSQ
                     ATEIARLSAELDKVRRESQEQIDRLRADHGKSHDAQVQSLHVQLEELRKKILAEQATW
                     ETQRKQLEAQAKAAEQKAAQGGKGKGDGEAEKELVKLRAEIKQLESSLKEAEQQARRG
                     GGGTDEDLKKRFEMAVQDVRELKTKNAELTEQLNKARTSGGGAAVAVGSDWESMKKRM
                     MLQLADDFDEKNDKQKADKLTIEGAMKITDDVVAEKEKEIQELRRTLESQAQSVGDVA
                     VGAAAIAQMLDSDELVRQERETLHKLQESLREQLKKAEIDISLERAKLARERQELDEK
                     LRTIEAERPAPDEGTKGKKGGAGRRWLTRLGLGDSKGE"
     gene            complement(4228..6096)
                     /locus_tag="VFB80_10045"
     CDS             complement(4228..6096)
                     /locus_tag="VFB80_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34152.1"
                     /translation="MKYKAIMLLATAMLLATSCKPQPVATTSAAPAPQPANSGSASEL
                     PQAVDALRKLAEGWSPEGVNRTYFYFNQWLASYEPAKAAWQPDKLLEHLPLSLKNTPG
                     LDRLDRNRFEKDDALTLWAALQMPPEMKPDDAKKFREAMVSLIPAMQVGPWLSDMAYF
                     QQTLWLHDIAERVAKQSPPAKLKPWLKEFETGVGLPEAEQLQAAERLFDWTIRNVQLD
                     PLPPMPKAPAATVDPTTKDRLDPVSPAQLGEVGPGYAQLPVQVLLYGHADAHERARIF
                     IQLCRQAGIDAVMLAVNDEASPIPRPWTAAVLVGGQLYLFDTALALPLPGPEGQGIAT
                     LDQVAANPELLKQLDVEGQPPYPVGPQDLRAVIALIDAEPAALSRRMQHLQAGLPRES
                     RLVLSVRPSELEAKIKNGKHNKAVSTVSLWRAPLEAIVYQVGRLATFPTNKAKAAEFD
                     LVQNIFSPGQPLHLARNLHLQGRLTDGEREPGARTLYLMCRKPDRDIQAMRTNPFFRA
                     AVGLQDNPNAKASERDAMLEYWTRKLQTEKQHATYWLGLSYYEEGNLNTTLTFLGQRT
                     ISAATQSPWESGARYNLARCYEDLGQLELARQWLESDKDSPQRTGNLLRAKWLAGR"
     gene            complement(6093..6932)
                     /gene="kdsA"
                     /locus_tag="VFB80_10050"
     CDS             complement(6093..6932)
                     /gene="kdsA"
                     /locus_tag="VFB80_10050"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZN34153.1"
                     /translation="MPNSTNNPATIGPHQCGRGQALLVIAGPCVIESEELTLSIARRL
                     KEIVAGLPVQLVFKASFDKANRTSGAAFRGAGLAAGLQVLAAVKSATGLPVTTDIHES
                     HQAHPVAEVCELLQIPAFLARQTDLLVAAAQTGRAVNVKKGQFMAPWDMKNVLAKLND
                     AGCKNVLLCERGTFFGYGRLVNDMRALPEMRSLGAPVIFDATHSVQEPGGLGSATGGN
                     RAMVEPLARAAVAIGVDGLFFEVHPDPDRAPSDGPNMLRLDEFPAVLRRLLALRKTVE
                     SFA"
BASE COUNT         1235 a         2358 c         2354 g         1060 t
ORIGIN      
        1 gatgcctcgc ggattgcgcg ggccgcagac gagcggatgg cgctggtgcg gagctaccag
       61 gtcggcccgc ggggcgccta tacgcagagg ctgctcaatg aattgtcggc cgcccgcatt
      121 tgcctgctca cgccggccag caaagcgcag tacgacgcgg ccttgggaca gcggatgcgg
      181 gccgagccgc aggctgatgc gccgccgcgc ttgccgccgc cagctccacc gccgacggaa
      241 tccaccgcgc tgccaagcgc cgcggcagac gatgcatcgg ccacgccgca cggctggccg
      301 cggctggttg ccgcgatgct ggcggtggcg gcgctggtgc tctgcgcgat cggcggcggg
      361 atttggatca ggtcttattg gccgcccgcc agaccggtgg aaccgccgcg gccggtcgtc
      421 accgagcccg cgccaccgca accgatcatc gtgttgcagg aaggcagcgg cgaagtcgtg
      481 ctttcggccg cgactgctac gctcgccggg gcggtggaat tgcaggtcgt cgggacggag
      541 gaagtgctgg cccattggac gactccggag gatcgggccg tgtggcattt ccggctcgtg
      601 aaaccgggcc ccttttacgc ggaattgacc tgtgccacca gcgccgaggc ggagggtttc
      661 gaattggagc tcgtcgttga cgatcgggcc acggcgcacc tgctgggccc ctccggcggc
      721 ttcgatcagt ttcgcaccga caaggttccg ctcgtcatcg cgagcggcgg cgagcacacg
      781 ctgacactgc ggctggcgaa ggcgccgccg gccgattggc tggttctgaa gagcgtacgg
      841 cttgtgccgg tcagcgcgga cgcgccgccc gatgcgccgg cggaagcgcc tgcgccctcg
      901 gcgccttgaa ctaagcttgc cgaaacggac ccgacagttc gtcgctccga gatcagcagc
      961 catgcgcctt tgtttcgctt gcctcgtgtt ggcgtccgcc agcgggcttt ctgccgccga
     1021 gccgctgctg cggttcgacc tgcccggccg gaccgtggaa ggaacgcccc tcgcctggtc
     1081 ggaaaaggaa atcttcctgc tggcccgcga cggccagctg atcgagtttc cgcctgagca
     1141 agccaagaat ccgtcgaaac tgtccgacgg gttcaagagc ctgtcgcagg cggaaatccg
     1201 cggcctcttg cttgccgagt tcggccaggg ctacgaggtt tcgggcgtcg gccattacct
     1261 cgtcgtgcac gccaccggcc agcgcgacca gtgggcgccg cgattcgaag agctgtaccg
     1321 atcgttccgg cattacttcg cggctcgcgg ctggagcctg accgagccgc gctttccgct
     1381 ggtggccgtc gtctatccgc ggcaggccga tttcgcgcgg caagccgccc gcgacggcct
     1441 gacgcaggcc ggcggcgtgc tcggctacta ctcgccgatg accaatcgaa tcctgatgtt
     1501 cgacgccggc agcagcccga actggtcgaa cgatgccgag acgatcatcc acgaggcggc
     1561 gcatcagtcg gcgttcaaca ccggcgtcca cacccgcttt agcgcgacgc cgcggtgggt
     1621 cgtcgagggg ctggggatga tgttcgaggc ccgcggcgta tggcagtcgc ggacttatct
     1681 tcaccagggt gaccgcatca accgcggacg cctgcaagcc taccgccagc gcgcgaggag
     1741 ccgcccggcc gacgccatcg cccagctcgt ctcgtccgac cggccgttct cgtccgaccc
     1801 cgacggggcc tacgccgagg cctgggcgct gtcgttcttc ctgatcgaat cggagccgaa
     1861 gaagtaccag gagtacctgg cgaaaaccgc cgccctggcc cccttcgccg cctatccctc
     1921 ccctcagcgg ctgaaggact tcaccgacac cttcgggagc gacctcaagc tgctcgacgc
     1981 ccgaatgcaa cgctttgtgg cgggattgaa gtagggcgaa tcgctgacga tttgccagcg
     2041 tttccctgca cgtgcccgag actgaccgac caaccgtccg actgccgcta aattcggtat
     2101 ctcgcgctcg tccgctgcaa ctgcgccagg ccgcagcgct gtctttgacg caccccaccc
     2161 catgacctat agaactctgg ggtgtctcgc cgcgaatggg tggcgaacct ccgatttccc
     2221 ctgcgcaggt tgccgacatg gaagagaccc ttcgtgaggc tccgccctcc gacgactggc
     2281 gaaaatccct cgcccaaacc atcgaacaac agcgccacaa agtgggccag cggatcgccg
     2341 gccagcggga gcgcttgcgc gagctggaag gcaagatcgc ccagcacctg ggcgaggtca
     2401 cgcaggagct gcagagcaag caggacgccg cccgcgaggc cgtggccaag tggcaggccg
     2461 acgaagcctc cgccaaacag cgcgacgccg acctgacgcg gcgggaactc gaagtatcca
     2521 agcgcgaatc cgacctcaag gccctcgccg cccacggcag caacggcagc ggtaaagagc
     2581 accagcacct gctcgaggac ctgaccaagc ggctgaccga gctcgaccag cggcacgcca
     2641 aggtcgccac cgaagagaag cggctggtga cgcggcagca ggagctcagc ctccgcaagt
     2701 cggagctcga ccagctgcag gccggcctgt cggggcaaag cgccgcccac caaagccggg
     2761 cggacgagct ggcccggcaa gccgcggagc tcgaaaaggc acggcaagcg caccagaccg
     2821 aagcccagcg gcaccagcag caacaaagcg atgtccagcg gctgcagagc gagctcaagc
     2881 tcaaggccca gtcgctggcc gaacaggagc gtgaaacgca gcgccagcgc cggcacatcg
     2941 cccagcagct gcgctcccgc aagaaagaac agaacgccga aatcctggcc accggcagcg
     3001 gccaggaaca cgagctgaag ctgaagctca cggaattgca gggcaagctc gaccgcctgc
     3061 gcgaggagcg cgaccacgtg cagcggcagc tggagcaggc ccagcggaag cagagcgagc
     3121 tggagtccga gcggcagcgg ctgcagacgg acttgcagcg gcacttggaa gaagccggcc
     3181 gcaaggccga ggcggcccgc ctcgacatgg cccgccgcac gtccgagtca caatcccagg
     3241 cgacggaaat cgcccggctc tccgccgaac tcgacaaggt ccgccgcgaa tcgcaggagc
     3301 agatcgatcg gctgagggcc gaccacggca agtcgcacga cgcgcaggtc cagtcgctgc
     3361 acgtgcagct cgaggagctg cggaagaaaa tcctcgccga gcaggcgacc tgggagaccc
     3421 agcggaaaca gctcgaggcc caggccaagg ccgccgagca gaaggctgcc cagggcggca
     3481 agggcaaagg cgacggcgaa gccgagaaag agctcgtcaa gctccgcgcg gagatcaaac
     3541 agctcgaatc ctccctgaag gaggccgagc agcaagctcg gcggggcggc ggcggaacgg
     3601 acgaggacct gaagaaacgg ttcgagatgg ctgtccagga cgtccgcgag ctgaagacca
     3661 agaacgccga gctgaccgag cagctgaaca aggcccggac ttccggcggc ggcgcggctg
     3721 tggccgtcgg cagcgactgg gagtcgatga agaagcggat gatgctgcag ctggcagacg
     3781 acttcgacga gaagaacgac aagcagaagg ccgacaagct gacgatcgaa ggcgcgatga
     3841 agatcaccga cgatgtcgtc gccgagaagg agaaggaaat ccaggagctg cggaggacgc
     3901 tggagagcca ggcccagagc gtcggcgacg tggccgtcgg cgcggcggcc atcgcccaga
     3961 tgctcgacag cgacgagctg gtccgccagg agcgcgagac gctgcacaag ctccaggaga
     4021 gcctccgcga gcagctgaaa aaggcggaaa tcgacatctc tctcgagcgg gccaagctgg
     4081 ctcgcgaacg gcaagagttg gacgaaaagc tgcggacgat cgaagccgag cgccccgcgc
     4141 cggacgaagg gaccaaaggc aaaaagggcg gcgccggccg ccgctggctc acccgcttgg
     4201 gattgggcga ctccaagggc gagtaattca gcgaccggcc agccacttcg cccgcaacaa
     4261 gttcccggtg cgctgcggcg agtccttatc ggactcgagc cactggcggg caagctcgag
     4321 ctggcccagg tcttcgtagc agcgggccag gttgtaccgc gcaccgcttt cccagggact
     4381 ttgcgtggcg gccgaaatcg tccgctgacc gaggaacgtc agggtcgtgt tcaaattccc
     4441 ctcttcgtag tacgacaggc cgagccaata ggtcgcgtgc tgcttctcgg tctgaagctt
     4501 ccgcgtccaa tattccagca tcgcatcgcg ctccgatgcc ttggcgttgg gattgtcttg
     4561 caggccgacg gctgcccgga aaaacgggtt ggtcctcatc gcctggatgt cgcggtccgg
     4621 cttccggcac atcagataca gcgtgcgggc ccccggctcc ctctcgccgt cggtcagccg
     4681 cccctgcaaa tgcaggttgc gggccaggtg cagcggctgc ccagggctga aaatgttctg
     4741 caccaggtcg aattccgcgg ccttcgcctt gttcgtcggg aacgtcgcca ggcggccgac
     4801 ctggtacacg atcgcctcca gcggcgctcg ccacaagctg acggtgctga ccgctttgtt
     4861 gtgcttgccg ttcttgattt ttgcttccag ctcgctgggc cgcacggaga gcaccagccg
     4921 cgattcgcgg ggcaggccgg cctgcaagtg ctgcatccgc cgcgagaggg ccgccggctc
     4981 cgcgtcgatc agcgcgatca cggctctgag gtcctgcggg ccgacgggat agggcggctg
     5041 cccctccacg tcgagctgct tgagcaattc gggatttgcc gcgacctggt cgagcgtcgc
     5101 gattccctgg ccctccggcc caggcagcgg caaggccagc gccgtgtcga acagatagag
     5161 ctgcccgccg acgagcaccg cggccgtcca ggggcgcgga atcggcgagg cttcgtcgtt
     5221 gaccgccagc atcaccgcgt cgatccccgc ttgccggcag agctggatga agatgcgggc
     5281 ccgctcgtgc gcgtcggcgt ggccgtagag cagcacctgc acgggcagtt gggcgtagcc
     5341 ggggccgacc tcgccaagct gcgccgggct gaccggatcg agccggtcct tggtcgtcgg
     5401 atcgacggtc gcggcgggcg ccttgggcat cggcggcagc gggtcgagct gcacgttgcg
     5461 gatcgtccag tcgaacagcc gctcggccgc ctgcaactgc tccgcctcgg gcaagcccac
     5521 gccggtttcg aattctttga gccacggctt gagcttcgcc ggcggcgact gtttcgcaac
     5581 ccgctcggcg atgtcgtgca gccacagcgt ctgctggaaa taggccatat cgctcagcca
     5641 cgggcccacc tgcatcgccg ggatcaggct gaccatggct tcccggaact tcttggcatc
     5701 gtcgggcttc atttccggcg gcatttgcag cgcggcccac agcgtcagcg cgtcatcttt
     5761 ttcaaaccgg ttgcggtcga gccgatccag ccccggcgtg ttcttcagcg acagcggcaa
     5821 gtgttcgagc agcttgtcgg gctgccaggc cgccttcgcc ggctcgtacg acgccagcca
     5881 ctggttgaaa tagaaatagg tccgattcac gccctccggc gaccatcctt ccgccagctt
     5941 gcggagcgca tcgacggcct gcggcagctc gctcgctgag ccgctgtttg ccggctgcgg
     6001 agcgggcgcg gcgctggtcg ttgcgaccgg ctgcggcttg cagcttgtcg cgagcagcat
     6061 ggcagtcgcc aggagcatga tcgctttgta tttcacgcga agctctccac ggtcttgcgc
     6121 agggcgagca gccgccgcaa cacggcgggg aattcgtcca gccggagcat gttcggccca
     6181 tcgctcggcg cgcggtccgg gtcggggtgg acttcgaaga acaggccgtc aacgccgatc
     6241 gccaccgcag ctcgcgccag cggctcgacc atcgcccggt tgccgccggt cgcgctgccc
     6301 agtccgccgg gctcttgcac gctatgcgtc gcgtcgaaaa tcacgggcgc tcccagcgac
     6361 cgcatctcgg gcagcgcccg catgtcgttc acgaggcggc cgtaaccgaa gaacgttccc
     6421 cgctcgcaga gcagcacatt cttacagccg gcgtcattca gcttggccag cacgtttttc
     6481 atgtcccagg gggccatgaa ctgtcccttc ttcacgttca cggcccggcc ggtctgggcc
     6541 gcggcgacga gcaagtcggt ctggcgggcc aggaaggccg gaatctgcaa tagctcgcaa
     6601 acctcggcga cggggtgagc ctggtgcgac tcgtgaatgt cggtcgtcac gggcaaaccg
     6661 gtcgccgact tcaccgcggc caggacctgc agccccgcgg ccagtcccgc gccgcgaaac
     6721 gccgcgccac tggtgcgatt ggccttgtcg aacgaggctt tgaagaccag ctgcaccggc
     6781 aatcccgcca cgatttcttt caggcgcctg gcgatcgaca gggtcagctc ctcgctctcg
     6841 atgacgcacg gcccggcgat gacgagcagc gcttgcccac ggccgcattg gtgcgggccg
     6901 atggtcgccg ggttgttggt tgagttgggc atcgcgtggc gctgtaggga acgccctctg
     6961 tggcgttccg gatcgttcga tttgttagcc cgcagccgga ggcgcag
//
LOCUS       DASRJB010000381         8841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2929207, whole genome shotgun sequence.
ACCESSION   DASRJB010000381 DASRJB010000000
VERSION     DASRJB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929207"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFB80_10055"
     CDS             complement(<1..894)
                     /locus_tag="VFB80_10055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN34154.1"
                     /translation="MLARYLLRRLAIGCLLLVAAGSAAGFAAELDPQKLAAIAPRMEQ
                     FVKEGTIAGAVTVVGNAQGIAQLDAVGLQSLESKQPMPRSAVFRIASMTKPITAIGIM
                     QLQDQGLLSVEDPVEKHLPEFKGQLLIDSREGERVTLKKPPRPITIRDLLTHTSGLPG
                     GFPAGYSDLYFTRQHTLKEATLAQAQRPLEFEPGSKWAYCNAGIDTLGRIIEVKSGQK
                     YEDYLAARVFKPLGMTDTSAYPDEKQLARLAGLYGQREGKLYFADYVLIGPTKGARHP
                     IPAGGLFSTGADLAKLYQAMLN"
     gene            complement(956..2215)
                     /locus_tag="VFB80_10060"
     CDS             complement(956..2215)
                     /locus_tag="VFB80_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34155.1"
                     /translation="MAAATPKANSPPAPPAPPVVAAPATATPAASQVATGQVEWALYE
                     NAEKGFKAVFPGRQPDSIDPLSAIEDPEQRKLAEAMTKEMTVLGATHGGRKFTLTCAP
                     LVLGGVAPGVYLDRMSAGLRILHQGFSIEPVSAADKSAPVRDYVLTKSDAGKLLRVVV
                     GQGQVFHLLIEGESGLAFSDPAAQQFFGKFQCAGVSAAAVSTSASGESQGSTKRKKSA
                     AAPPDAPDGLDWRPFQGNKVAFTINFPGVTPQAEDPLSLILESNRQNQNDNWARDGVV
                     VESFAAQVGERRYGVTAFRDPNRRQEGSIRFAGQMQGLLRTFAQDIHQEVRDHVVRSR
                     RPPSKLEILATSSLAMRDGSKAIVRQCCLGHYGFVVRVEGPAAMEDLDPQVHKFLDSL
                     QPPPDAAPLPLNVRPLPGPKPEQSKQK"
     gene            complement(2667..3989)
                     /locus_tag="VFB80_10065"
     CDS             complement(2667..3989)
                     /locus_tag="VFB80_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34156.1"
                     /translation="MNAKLHRRSFLESSAAAGAAFAAGSYFVSESGAQESKSLGSRIA
                     VGVMGANGRGTALAEGFMGQPNCEVAYVCDVDENVLNRVSKAVADKQERKPQGVKDFR
                     RMLDDKSLDAILVAAPNHWHAPATILGCAAGKHVYVEKPCSHNPREGELAVEASRKYK
                     RVVQMGSQRRSGPAIKEAIAKLHAGEIGRVIYSRAWYNNNRPSIGHGQEAPPPANLDW
                     TLWQGPAPERPYMKGDVGTGNFIVPYNWHWHWHWGNGELGNNGIHALDMCRWGLAVDY
                     PIKVTSLGGKYRHDDGQETPDTHTVTFNFPGKKSIMWEGLSWSPYGPGGLSFGASFHG
                     EQGSIVIDGSGYKLYDLKNKEMGGRTTSLGEREHFANFLDCIRSGRRPNADIEDGHKS
                     TLLCHLGNIAYRVGRVLTCSEKNGHIQGDDEAMKLWTREYRPGWEPKV"
     gene            complement(4079..7033)
                     /locus_tag="VFB80_10070"
     CDS             complement(4079..7033)
                     /locus_tag="VFB80_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN34157.1"
                     /translation="MIAAVLAARPAAGQLPPKNVPHDGYWPCFVAYYDGDFRAALQDF
                     RGAAQGGYASVEGRWIDSICYYTMLGECYYQMGNNADALAQYEAALKLFLVNRDWMIR
                     VEFPATISADTNVGKTVAWGPSTRRLVRGQFPEKFQSLQGRLDNENVLKTGGVVANPQ
                     LVPVYVSEIVRCTTLAMSRRRQIAGPSCEHDRLTLDLLNALSRRPGPPNHWSQCWLEL
                     QLGVAYAAANKPDQAASELTRSLLAGGTFDHPLTCVGFLELARLAFEQGKYDPAIAYA
                     HEATISAALFDRYDVLEEAFRLAAKAHVVSGKKGIYPPLTAAVTTSKGVPMLHVSLLT
                     LLAEQYCSLGQMPAAATAIGQAKSGMNRRDMLQGALGARMYYQAARIALQSGEAKAGA
                     SALTAALAYQKAGSLGLFQIALADKLFAAGAWTERIADLVYAEVLREPTRADWTVEPL
                     DTLAVGISPHPLPFEHWLEVALVRKEYEKALNITDRLRRHRFYASQALGGRLLALRWV
                     LEAPTEALGKEALLQRQDLLVRYPKFADLSKQAADIRSKLQGLPLIAADETQRREQTA
                     LLSELSTISFAQEALLQMIALERVPSEFAFPPLRDTKLIQAELPQGTLVFDYLVTSRH
                     VHAFAMTNDRLGYFTVSQPAKLKADLGEMLRLMGHHDRNQPVSAEDLKANGWRVAAGR
                     MVGQLTNNTQQDDWAKYRELVIVPDGPLWYLPFETLPVPIEGGINGPLLLQIPIRYAP
                     TLSLAAPDRRQIRPVTRTAVVGGKLLSRDDDEFAKKSVAAVVAALPGSVELNADSLRA
                     TSATLSSAFDRLVVMRDSEDPERLPFGWAPLALDAGKPGSTLADWGLLPFSGTEQVVF
                     PGFHTPAEFGLKRGGTGEEVFLAVCGLMASGSRTILLSRWRVGGQSTVDLMREFVQEL
                     PHESAAAAWQRSVRLAASRDLDPATEGRLKANGAALGVKADHPFFWSGYLLVDTGVKP
                     PAIPPKAAAAAR"
     gene            complement(7166..7573)
                     /locus_tag="VFB80_10075"
     CDS             complement(7166..7573)
                     /locus_tag="VFB80_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34158.1"
                     /translation="MSLNPYAAPQPVVAEASLARDASLTPFLAWERLRVWFNTILVLV
                     VVVSALPQRELWFSYRLNYYCIEGAILANICFCVGLVARSAALGAVRPGHAAGGAPGP
                     RGHRRPAQPAVALSPVSHSDSAAAGRPLVGAAV"
     gene            7645..8658
                     /locus_tag="VFB80_10080"
     CDS             7645..8658
                     /locus_tag="VFB80_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN34159.1"
                     /translation="MSEQPSVILSGFGDEAAIQKTAEQQFAAFAAIGLQYYSLRFIDA
                     GQGIKNVMQLSKAEITKIRHLEDAYDMNVATLGSPIGKVKLLDQEDGTKNRYVPFKKY
                     LSDEVKKACELAHAFETKLIRGFSFYHPKGTDPWRHVPQAVDQLGQIAETCHRSDLTF
                     GLEVEANLVGQNGELMAELHKQVDHPALLTIFDAANISTQGFGPGEVFQQYEKMKKSL
                     GWMHIKDYRHPQRAERTTHVDEEALKHFVPADRGDSGHELVLRDFREMIPVLEKKLKK
                     RGVPGVFLDLEPHVKGGGQFGGFSGPDGMGVALRGLCRVLDYLDIGYHLRDFDDVRAA
                     RGF"
BASE COUNT         1500 a         2872 c         2971 g         1498 t
ORIGIN      
        1 gttcaacatc gcctggtaga gcttcgccag gtccgcgccg gtggagaata agccgccggc
       61 tgggatagga tgtcgtgcgc ccttggttgg accgatcagg acgtaatcgg cgaagtagag
      121 cttgccctcg cgctggccgt agagaccggc caggcgggca agctgcttct catcgggata
      181 cgccgacgtg tccgtcatgc cgagcggctt gaaaacgcgg gccgcgagat agtcctcgta
      241 cttctggccg ctcttgacct cgatgatccg gccgagcgtg tcgatgccgg cgttgcagta
      301 ggcccatttg ctgcccggct cgaactccag cggccgctgg gcctgggcca gcgtggcctc
      361 cttcagcgtg tgctgccgcg tgaaatagag gtcggaatag cccgccggaa agccgcccgg
      421 caggcccgag gtgtgcgtca agaggtcgcg gatcgtgatc ggccgcggcg gcttcttgag
      481 cgtgactcgc tcgccctccc gcgagtcgat cagcagctgc cccttgaact cgggcaggtg
      541 tttttcgacc gggtcctcga ccgagagcag cccctggtct tgcaattgca tgatgccgat
      601 cgccgtaatc ggcttggtca tcgaggcgat gcggaagacc gcactccgcg gcatcggctg
      661 cttggactcc agactctgca agccgaccgc gtcgagctgc gcgatgccct gagcatttcc
      721 cactaccgtt actgcgccgg cgatcgtgcc ctccttgacg aactgctcca tccgcggcgc
      781 gatcgcggcg agcttctgcg ggtcgagttc ggcggcgaag ccggcggccg agccggcggc
      841 aacgagcagc aggcaaccga tcgccaggcg gcgtaaaaga tagcgggcaa gcatttcggc
      901 tccttcatct cgtgaatttc gcgatctgaa ccgtgcattt gcggacacgg cggaatcact
      961 tctgcttcga ttgttccggt ttgggtccgg gcagcggcct gacgttgagt ggcagcgggg
     1021 cggcatcagg cgggggctgg aggctgtcga ggaatttgtg gacctgcggg tcgaggtcct
     1081 ccatcgcagc cggcccttcg acgcggacca cgaatccata gtgccccagg cagcactggc
     1141 ggacgatggc cttgctcccg tcgcgcatcg cgagggagga tgtggcaaga atctccagct
     1201 tcgacggagg cctgcgcgac cggacgacgt ggtcgcggac ctcctggtgg atgtcctggg
     1261 caaaggtacg gagcaggcct tgcatctgcc cagcgaagcg aatcgatcct tcttgccgcc
     1321 gattgggatc gcgaaaagcg gtgacgccgt agcgccgctc gccgacctga gccgcgaagg
     1381 actccacgac cacgccgtcg cgggcccagt tgtcgttttg attctgacga ttcgactcga
     1441 gaatcagcga gagcggatcc tctgcctgcg gtgtgacgcc cggaaagtta atcgtgaacg
     1501 cgaccttgtt gccctggaac ggccgccagt cgaggccgtc gggtgcgtcg ggcggtgcag
     1561 cggcggattt ctttcgtttt gtcgaaccct ggctttcgcc gctggcgctg gtcgaaacgg
     1621 ccgcagcaga cacgccggca cattggaact tcccaaaaaa ctgctgcgcg gccggatcgc
     1681 tgaaggccag gccgctctcg ccttcgatca gcaaatgaaa cacctgcccc tggccgacga
     1741 cgacgcggag gagcttgccc gcgtcgctct tcgttaggac atagtcccgc acgggcgcgg
     1801 atttgtccgc tgcggacacc ggctcgatcg aaaacccctg gtgcaggatt ctcaagccgg
     1861 ccgacatgcg gtccagatag acgccggggg cgacgccgcc caacacgagc ggcgcgcacg
     1921 tcaacgtgaa tttgcgaccg ccgtgcgtcg cgccaagcac cgtcatctcc ttcgtcatcg
     1981 cctccgccaa cttccgctgc tccggatctt caatcgcgga cagcggatcg atcgagtccg
     2041 gctggcggcc gggaaagacg gccttgaacc ccttctcggc gttctcgtac agcgcccact
     2101 cgacctgccc cgtcgcgact tgggaggcgg ccggcgtcgc ggttgccggc gcggcgacca
     2161 ccggcggagc aggcggagca gggggcgaat tcgctttcgg cgtcgctgca gccaccgcac
     2221 gatccgccgg cgatcggccg ctgggccagt tcaggacaat gacaactgcg gccacgacgc
     2281 cgaccaaaag caggccgcct gcgccgatcg cgcccgccag aagccagggc agcgcggtcc
     2341 cggggcggcg cgcctcaggg gcagccgact ctgcggacgc tggagcctga gaaacgatga
     2401 tgggcgtcaa cggaacaggg gctggcctgg cttggctgac cgcgggcgcc gcgctggccg
     2461 ccggtacggt gacggtcgcc tgacacttcg ggcacttgac gcgtttgccc ctcagattgt
     2521 cgggtgcatt cagcttcgcc tgacacgcgg gacattggat ggaaagcggc atgactgaag
     2581 cgcctgggca caagcaaaat gcgacttttc cccttgccga ctggcaccac caaggtaagc
     2641 accgttagac gcaatcgcca gcgccctcag accttcggct cccagcccgg ccggtattcg
     2701 cgggtccaga gcttcatcgc ttcgtcgtcg ccttggatgt ggccgttctt ctcgctgcag
     2761 gtcagcacgc ggccgacgcg gtaggcgatg tttccgaggt ggcacaggag cgtgctcttg
     2821 tggccgtcct cgatgtcggc gttgggccgc cggccgctgc ggatgcagtc caggaagttg
     2881 gcaaagtgct cgcgctcgcc gaggctcgtc gtgcggccgc ccatctcctt gttcttcagg
     2941 tcgtaaagct tgtagcccga gccgtcgatg acaatcgagc cctgctcgcc gtggaagctc
     3001 gcgccgaacg acagtccgcc ggggccatag ggcgaccagc tgaggccctc ccacatgatc
     3061 gatttcttgc cggggaagtt gaacgtgacg gtatgcgtgt cgggcgtttc ctggccgtcg
     3121 tcgtggcggt acttgccgcc gaggctggtc accttgatcg ggtaatcgac cgcgaggccc
     3181 cagcggcaca tgtccagggc gtggatgccg ttgttgccca gttcgccgtt gccccagtgc
     3241 cagtgccagt gccagttgta cggcacgatg aagttgccgg tcccgacgtc gcccttcatg
     3301 tacggccgct cgggggccgg gccttgccag agcgtccagt cgaggttggc cggcggcggc
     3361 gcttcctggc cgtggccgat ggaggggcga ttgttgttgt accaggcgcg ggaatagatc
     3421 acgcggccga tctcgccggc gtgcagcttg gcgatcgctt ccttgatcgc cgggccgctg
     3481 cggcgctgcg agcccatctg cacgacccgc ttgtacttgc gcgaggcctc gaccgcgagt
     3541 tccccttcgc gcggattgtg gctgcacggc ttctcgacat agacgtgctt gcccgccgca
     3601 cagcccagga tcgtcgccgg ggcgtgccag tggttcggcg cagccaccag gattgcatcc
     3661 agcgacttgt cgtcgagcat ccgccgaaaa tccttcacgc cctgcggctt gcgctcctgc
     3721 ttgtcggcga ctgccttgct cacgcgattg agcacgttct catccacgtc gcagacatag
     3781 gcgacttcgc agttgggctg ccccatgaag ccctcggcca gcgccgtgcc gcggccgttg
     3841 gcgcccatca cgcccacggc gatccgcgag cccagcgact tgctctcctg ggccccactt
     3901 tcggagacaa aatacgaacc cgcagcaaat gcggcccccg ccgcggccga cgattcgaga
     3961 aacgagcggc gatggagttt agcgttcatc gagtgacctc caggcagtgg cgggacagga
     4021 tcgaaagcgc gcaggccggt aatgtagcac agcagcgcgc gaatctcacg gctaggcgct
     4081 accgcgccgc agcggcggct ttgggcggaa tcgccggggg tttcacgccg gtgtcaacca
     4141 acaggtagcc ggaccagaag aagggatggt cggctttgac ccccagagcg gcgccgttgg
     4201 ctttcaagcg gccttcggtc gcgggatcga ggtcgcggct ggcggccagg cggacgcttc
     4261 gctgccaggc ggccgcggcc gattcgtgcg gcaattcctg cacgaattcc cgcatcagat
     4321 cgaccgtgct ttgcccgccc acgcgccatc gcgaaagcag gatcgtgcgg ctgccgctgg
     4381 ccatcaggcc gcagacggcc aggaacacct cttcgcccgt gccgccgcgc ttcaggccga
     4441 actcggccgg cgtgtgaaaa ccgggaaaca cgacctgctc ggtgccgctg aagggcaaca
     4501 gaccccagtc ggccagcgtg ctgccgggct tgccggcatc gagcgccagc ggggcccagc
     4561 cgaacggcag ccgctcggga tcctcgctgt cgcgcatcac cacgaggcga tcgaaggccg
     4621 aggaaagcgt ggcggaagtg gcccgcagcg aatcggcgtt cagctcgacg ctgccgggca
     4681 gggcggcgac gacggccgcg acggatttct tggcgaattc gtcgtcgtcg cgcgagagca
     4741 gcttgccgcc cactaccgcg gtccgggtca cgggccggat ctgccggcgg tcgggcgcgg
     4801 ccagcgacag cgtcggagca tagcggatgg ggatttgcag caagagcggc ccgttgatgc
     4861 cgccttcaat cggcacgggc agcgtttcga acggcaaata ccagaggggg ccgtcgggaa
     4921 cgatcaccag ctcgcgatac ttggcccaat cgtcctgctg cgtgttgttg gtcaattgcc
     4981 cgaccatccg accggccgcg acgcgccagc cgttggcctt gagatcctcg gccgacaccg
     5041 gctgattgcg atcgtgatgg cccatcaggc ggagcatctc acccagatcg gccttcagct
     5101 tcgccggctg cgagacggtg aaatatccca ggcggtcgtt ggtcatcgcg aaggcgtgaa
     5161 cgtgccgcga ggtgaccagg tagtcgaaga ccagcgttcc ctgcggcaac tcggcctgga
     5221 tcagtttcgt gtcgcggagc ggcggaaacg cgaattcgct ggggacccgc tccagggcta
     5281 tcatttgcag caaggcttcc tgggcgaagc tgatcgtcga taactcgctc aggagggccg
     5341 tctgctcgcg gcgctgcgtc tcgtccgcgg cgatcagcgg caggccctgt agcttgctgc
     5401 ggatgtcggc ggcctgtttc gacagatccg cgaacttggg atagcggacg agcaggtcct
     5461 gccgctgcag cagagcttcc ttgccgagcg cctccgtagg cgcttcgagc acccatcgca
     5521 gcgcgagcag ccggccgccc agggcctgcg aggcatagaa gcgatgccgg cggaggcggt
     5581 cggtgatgtt cagggccttc tcgtattctt tgcggacaag ggccacttcg agccagtgct
     5641 cgaaggggag cggatgcggc gaaatgccca cggcgagcgt gtcgagcggt tcgacggtcc
     5701 aatcggcgcg cgtcggctcg cggagcactt cggcatagac caggtcggcg atccgctcgg
     5761 tccacgcgcc ggccgcgaac agcttgtcgg ccaaggcgat ctgaaagagg cccagcgagc
     5821 cggccttttg ataggccagc gccgccgtga gggccgacgc gcccgccttg gcctcgccgc
     5881 tctgcagggc gatgcgggcg gcctgatagt acatccgcgc gccgagcgcc ccctggagca
     5941 tgtcgcggcg gttcatgccg ctcttggcct ggccgatggc cgtggccgcg gcgggcatct
     6001 gacccaggga gcagtactgc tcggccagca gcgtcagcag cgaaacgtgc agcatcggca
     6061 cgcccttgct cgtcgttaca gcagcggtca gcggcgggta gatgcccttc ttgccggaga
     6121 caacgtgggc cttggcggcg aggcggaagg cctcttccag cacgtcgtag cggtcgaaga
     6181 gcgcggcgct gattgtcgcc tcgtgggcat aggcgatcgc cgggtcgtac ttgccctgct
     6241 caaaggccag gcgggcgagc tccaggaagc cgacgcaggt cagcggatgg tcgaacgtgc
     6301 cgccggccag cagcgagcgg gtcagctcgc tggcggcctg gtcgggcttg tttgccgcgg
     6361 cataggccac gccgagctgc agctcgagcc agcactggga ccagtgattg ggcggccccg
     6421 gtcgccgcga cagggcgttg agcaggtcca gcgtcaggcg gtcgtgctcg cacgacggcc
     6481 cggcgatctg tcggcggcgc gacatggcca gcgtcgtgca gcggacgatt tcgctgacat
     6541 agaccggcac cagctgcgga ttggcgacga cgccgcccgt cttcagcacg ttttcgttgt
     6601 ccagccgccc ttgcaggctc tgaaacttct ccgggaactg cccgcggacc agccgccgcg
     6661 tgctcgggcc ccaggcgacc gtcttgccga cgttcgtgtc ggcgctgatc gtggcgggaa
     6721 actcgacgcg gatcatccaa tcgcggttca ccaggaagag cttgagggcc gcttcgtact
     6781 gggccagggc atcggcgttg ttgcccatct ggtagtagca ctcgccgagc atcgtgtagt
     6841 agcagatcga gtcgatccag cggccctcca cgctggcgta tcccccctgg gccgcgccgc
     6901 gaaaatcctg tagcgcggcc cggaagtcgc cgtcgtaata ggcgacgaag cagggccaat
     6961 agccgtcgtg cgggacgttc ttgggcggca attggccggc ggcaggacgg gcggcgagaa
     7021 cggccgcgat catgcaagct gcccagatcg cagacgaggc ggtgcgtcca ctcatgagag
     7081 ctgttccggg gagaacgggc attcgccgaa ccgtggagaa ctctacgtcg ccccattccc
     7141 cggacggctg gccccaggcc gtccatcata ccgcggcccc caccagcggg cgaccagcgg
     7201 cggcagaatc ggaatgggag acaggagaca gtgctacagc cggctgagcg ggccgacgat
     7261 gcccgcgtgg gccaggagcc ccgccagcag cgtgcccagg ccgaacagca cccagcgcag
     7321 cggatcgcgc caccagcccc acacagaagc agatattcgc tagaatcgct ccctcgatgc
     7381 agtagtagtt cagccggtag ctgaaccaca attcccgctg cggcagcgcg ctgacgacca
     7441 cgaccagcac gagaatcgtg ttgaaccaga cccgcagccg ctcccacgcc agaaacggcg
     7501 tcagcgaggc gtccctggcc agggaggctt cggccaccac gggctgcggc gcggcataag
     7561 gattgagcga catcgcgcac ctcgagttac taaacccaga cagccgcggc aagtttcgtt
     7621 cccctttttc cttagccagc atctatgtct gaacaaccca gcgtaatcct gtccggtttc
     7681 ggcgacgaag cggccattca gaagacggcg gagcagcagt tcgcggcttt cgcggcgatc
     7741 ggcctgcagt actactcgct gcggttcatc gacgccgggc agggcatcaa aaacgtgatg
     7801 cagctttcca aggcggagat caccaagatc cgccacttgg aagacgccta cgacatgaac
     7861 gtggcgacgc tcggctcgcc gattggtaag gtgaagctgc tcgaccagga agacggcacg
     7921 aagaaccgtt acgtgccgtt caagaaatat ctgagcgacg aggtgaagaa agcctgcgag
     7981 ctggcccacg ccttcgagac gaagctgatt cgcggatttt cgttctatca tcccaagggg
     8041 accgatccct ggcgacacgt gccgcaggcc gtcgatcagc tggggcagat cgccgagacc
     8101 tgccaccgca gcgatctgac gttcgggctg gaggtcgagg cgaacctcgt ggggcaaaac
     8161 ggcgagctga tggccgaatt gcacaagcag gtcgatcacc cggccttgct gacgattttc
     8221 gacgccgcca acatcagcac ccagggcttc ggcccgggcg aggtcttcca gcagtacgag
     8281 aaaatgaaga agagtctcgg ttggatgcac atcaaggact atcgccatcc gcagcgggcc
     8341 gagcggacga cgcacgtcga tgaagaggcc ctcaagcact tcgtccccgc cgaccgcggc
     8401 gacagcggcc atgagctggt ccttcgcgac ttccgcgaga tgatcccggt gctggaaaag
     8461 aagttgaaga agcggggcgt gcccggcgtg ttcctggacc tggagccgca cgtcaagggc
     8521 ggcgggcaat tcggcggctt cagcggcccc gacgggatgg gcgtggccct gcggggcctg
     8581 tgccgcgtgc tcgactacct cgacatcggc tatcacctgc gggatttcga cgacgtgcgg
     8641 gctgcgcggg gattttgaac ccttgtacgg atcgccctcc gtggcgatcc gattcacccc
     8701 gcgcgaggaa cgccacagag ggcgttcctc tacacgtgca atggccgcat ccactcaacc
     8761 tgctggcgct tgccccaagt gtggcggcga ctcggtcgtt tgccggttca atttcttcga
     8821 ccgcggcgac ttgcaggtgc a
//
LOCUS       DASRJB010000382         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2364275, whole genome shotgun sequence.
ACCESSION   DASRJB010000382 DASRJB010000000
VERSION     DASRJB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364275"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFB80_10085"
     CDS             <1..1081
                     /locus_tag="VFB80_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZN34160.1"
                     /translation="FERIKRHQLYYQGPLSTRPDERPWSLHRNGHLGVAQWGGWVWSG
                     DTQSTWKTLEGQIAVGINHSLSLSPFWGSDTGGFYTTSELTGELYARWFQFSAFCPSF
                     RSHGRIWRLRTPWGWGLADMGFTEGQSDLPPQSALNDASIEPICRKYAELRYRLIPYT
                     YTLAAEARESGLPLMRAMWLHYPDEGQARGLGSQYLWGRDLLVAPVFTKGATTREVYL
                     PKGDWYDWWTGEKMAGGRTIQRPVDLETMPLYVRAGAILPIDPLRQYLSQPVSEPTTI
                     RVYSGADGEFRWYEDDGASQEYLAGKFAWTRLTWNDAARRLTIERDASAGTLLLPSRT
                     LNIELIPSGKRTAVTYDGRRAEVGL"
     gene            complement(1103..>2635)
                     /locus_tag="VFB80_10090"
     CDS             complement(1103..>2635)
                     /locus_tag="VFB80_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN34161.1"
                     /translation="LGRFDPFVSVNNNWDHLDLPTAVFLDPNNPGLGSAIVSGRSNVY
                     RLNVAVTKENPLGGQWRLGVDATESRFPISVLPLNPEQRSSADISYTQPLLQGFGPRA
                     NLAPVVLARIDTERSFFQLKGAVQQQVRGVIEGYWNLVSARTSAIVRRRQVEISQLGY
                     RRASAQFEIGLTDASGRAQARSTLAQFQAQLVAAEADVLQSEAALRNILGLPPWDEAQ
                     IVPTTEPTFERFEPDWNELVELAAQRRPDLVELKLILEADEQQLILANNNALPRLDAS
                     ALYRWNGLTGEMPNGNGLASGPGFNDWTLGVNFSVPVGLRTARAQLRQRELILARDRA
                     NLDQGLHAASHNLATTVRSLAQAYEQYLAFQEARNAAQANLDQQSAVNDVGRIDGLVY
                     RQAIADLGNSIVSEADSAANYNTLLAELELQSGTILETHGVRFYEERFGSIGPLGRPV
                     HPRDFPQSTPPTPNADRYPPGERPAERAFSLEQIERRTPGEQRPGSPEMLPQPLPRPR
                     ER"
BASE COUNT          401 a          876 c          893 g          465 t
ORIGIN      
        1 gttcgagcgg atcaagcggc atcagcttta ctatcagggt ccgctttcga cgcggcccga
       61 tgaacggcct tggagcctgc accgcaacgg tcacctgggc gtcgcccagt ggggcggttg
      121 ggtctggtcg ggcgacaccc aatcgacctg gaagacgctc gaagggcaga tcgccgtcgg
      181 catcaaccac tcgctcagct tgagcccctt ctggggctcc gataccggcg gcttctacac
      241 cacgagcgag ctgaccggcg aactctatgc ccgctggttc cagttctcgg ccttctgccc
      301 aagtttccgc tcgcacggcc gcatctggcg actgcggacg ccgtggggct ggggcctggc
      361 cgacatgggc ttcactgagg ggcaatccga ccttccgccg cagtcggccc tgaacgacgc
      421 ttcgatcgag ccgatctgcc gcaaatatgc cgagctgcgg tatcggctga ttccctatac
      481 ctacacgctg gccgccgagg cccgcgagag cggcctgccg ctgatgcggg cgatgtggct
      541 gcactatccg gacgaagggc aggcccgcgg cttgggctcg cagtatctgt ggggccgcga
      601 cctgctcgtc gcgccggtct tcacgaaagg cgcgacgacg cgcgaggtgt acttgccaaa
      661 aggcgattgg tacgactggt ggacaggcga aaaaatggcc ggcggccgga cgatccagcg
      721 cccggtcgat ctcgagacaa tgccacttta cgtccgagcc ggcgcgatcc tccccatcga
      781 tccgctgcgt caatacctgt cgcagcctgt cagcgagccg acgacgatcc gcgtttattc
      841 cggcgccgat ggcgaatttc gctggtacga ggacgacggt gcttcgcagg aatacctcgc
      901 cggcaaattc gcctggaccc gcctgacctg gaacgacgcc gcccgccggc ttaccatcga
      961 gcgcgacgcc agcgccggca cgctcctcct gccctcgcgc acacttaata ttgagctcat
     1021 tccctccggc aagcgcaccg cggtgaccta cgacggccga cgagccgagg ttggcctttg
     1081 aagttgaaaa ctctctgccg ggctaccgtt cgcggggacg cggcaacggt tgcggcagca
     1141 tttcgggact gccgggccgt tgttcgcccg gcgtccggcg ttcgatttgc tccaagctga
     1201 aggcccgttc ggcgggccgc tcgcccggcg ggtagcggtc ggcgttgggc gtcggcggag
     1261 tggactgtgg gaagtcgcgg ggatgaaccg ggcggcccag cgggccgatc gagccaaaac
     1321 gttcctcgta gaaccgcacg ccgtgcgttt ccagaatggt tccgctttgc aattccagct
     1381 ccgccagcag cgtgttgtag ttcgctgcgg aatcggcttc gctgacgatc gagttcccca
     1441 ggtcggcgat cgcctggcga tagacgaggc cgtcgatccg gccgacgtcg ttcacggcgc
     1501 tctgctggtc gaggttcgcc tgggccgcgt tgcgggcttc ctggaacgcg aggtattgtt
     1561 cgtacgcctg ggccaggctc cgcacggttg tggccaggtt gtgcgaggcg gcgtgcagcc
     1621 cctggtcgag gttcgcccgg tcgcgggcca ggatcagctc gcgctggcgc agctgcgccc
     1681 gcgccgtccg caggccgacg ggcactgaga aattcacgcc gagcgtccag tcgttgaacc
     1741 ccggcccgct ggccaggccg ttgccattcg gcatctcgcc cgtcaggccg ttccagcggt
     1801 agagggccga cgcatccagg cgcggcagcg cgttgttgtt cgccaggatg agttgctgct
     1861 cgtcggcctc gaggatcagc ttgagctcga ccaggtccgg tcgccgctgg gccgccagct
     1921 cgaccagctc gttccagtcg ggctcgaacc gctcgaacgt cggctcggtc gtgggaacga
     1981 tctgggcctc atcccaggga ggcagtccca ggatgttgcg cagcgccgct tcgctttgca
     2041 gcacgtcggc ttccgcggcg acgagctgcg cctggaactg ggccagcgtc gagcgggcct
     2101 gcgcccggcc gctggcgtcg gtcaggccga tctcgaactg ggcgctggcc cgccggtagc
     2161 cgagctgcga gatttcgacc tgccggcggc ggacgatcgc gctggtccgg gcgctgacca
     2221 ggttccaata gccttcgatc acgccccgca cctgctgctg caccgccccc ttgagctgga
     2281 agaacgatcg ctcggtgtcg atccgcgcca gcacgaccgg cgcgaggttg gcccgcggac
     2341 cgaagccctg cagcagcggc tgcgtgtagc tgatgtccgc cgacgatcgc tgctctggat
     2401 tgagcgggag cacgctgatg ggaaagcggc tctccgtggc atcgacgccc agccgccact
     2461 gcccgcccag cgggttttct ttggtcacgg cgacgttcag gcgatagacg ttgctgcgac
     2521 cggagacgat cgccgagccg agcccgggat tgttcggatc gagaaacact gccgtcggca
     2581 ggtcgaggtg gtcccagttg ttgttgaccg agacaaacgg atcgaagcgg ccgag
//
LOCUS       DASRJB010000383         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2929524, whole genome shotgun sequence.
ACCESSION   DASRJB010000383 DASRJB010000000
VERSION     DASRJB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929524"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VFB80_10095"
     CDS             complement(<1..1339)
                     /locus_tag="VFB80_10095"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34162.1"
                     /translation="MKTGPAERRCTGPLSCTGPLNRRQWLQLGGLSLGALSLGVQPTQ
                     AGLLAAERAGEIDRDFSVILFWANGGPSHIDLFDLKPDAPAEYRGPFRPIRTSVPGIE
                     ITELLPQLAKLADKFALVRSLHHERNEHSGGTHRFLTGYASRQANLNDAEYPELGSIV
                     AHELADRSPDVPPFVANTKLYGSGPAYLGPAYAPYMPAPNPASSTGNNTYDPIPIYPT
                     GGGNSALAIAAGGAVTLNRRLELLKDLDALPRAVDGGGQIAALDSFQQRAVAMLASPR
                     TRAAFDLAREDARTRERYGNTHFGRSLLTCRRLVEAGVRLVQCQAEFRLKPETGRTSN
                     WDDHSVNAHIFDAYREKLPSFDQSVSALIADLYERGLDDRVLFIFCGEFGRTPLIRNQ
                     DPSGRPGRDHWSRAMSVLLAGGGLKMGQVIGKTSSKAEEPVERVMDSNCLLATI"
     gene            complement(1346..2725)
                     /locus_tag="VFB80_10100"
     CDS             complement(1346..2725)
                     /locus_tag="VFB80_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34163.1"
                     /translation="MLIETEAHCMLHVRTGDRGRWSRRGFLQAGALGVGGLTLAELSR
                     LKAAGAVRPKAAGTSVILIWLSGGPGHMETWDPKPNAPAEYRGPLKAIDTNVAGVQFS
                     ELMPEQARLMDKLAVLRTVNHGTGDHTKGNHWMLTGFEGPAFNAPDNRDQRRPALGCA
                     AAKLRGPNKPGLPPYVAVPHLRGGTDNLFHYAAYLGGGWNPFIVNSDPNEKGFAVRNL
                     TLQGDLTLERVAERRTLREEFDRLKTAGERGGRELDDHQQAAFDLLTGPEVKSAFEIG
                     RESDKLRDRYGRHTFGQSALLARRLVEHGVTFVTVNCVPWDHHGSPGQYKTEEGARLL
                     IPPLDRAIAALIEDLVERGLYEKTLVVAMGEFGRTPRMNSNAGRDHWGNTFSVLMGCG
                     GMPMGQVIGRSSPRGEHVVERPLSPQDVAATVYHHLGIDCRAVSFRDALDRPMPLIDK
                     GEAIRELVG"
     gene            2917..2989
                     /locus_tag="VFB80_10105"
     tRNA            2917..2989
                     /locus_tag="VFB80_10105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2951..2953,aa:Glu,seq:ctc)
BASE COUNT          458 a         1018 c         1017 g          499 t
ORIGIN      
        1 agatggtcgc caggaggcag ttgctgtcca taacccgttc gacgggctct tcggccttgc
       61 tgctggtctt gccgatgacc tggcccatct tcaggccgcc gccggccagg agcacgctca
      121 tcgcgcggga ccagtggtcg cggccgggcc ggccgctggg gtcctggttg cgaatcagcg
      181 gagtgcggcc gaactcgccg cagaagatga acagcacgcg atcgtcgagg ccccgctcgt
      241 acaggtccgc gatcagcgcc gagaccgact ggtcgaacga tggcagcttc tcgcgatagg
      301 cgtcgaagat gtgcgcgttg accgagtggt cgtcccagtt gctggtgcgg cctgtttcgg
      361 gtttcaggcg gaattcggcc tggcactgca ccagccgcac gccggcctcg accagccggc
      421 ggcaagtcag caggctgcgg ccaaaatgcg tattgccgta gcgctcgcgg gtgcgggcgt
      481 cctcgcgcgc caggtcaaag gccgcgcggg tccgcgggct ggcgagcatc gccacggccc
      541 gctgctggaa cgaatcgagg gccgcgatct ggccgccgcc atcgacggcc cgcggcagcg
      601 cgtccaggtc tttcagcagc tccaggcggc gattgagcgt gaccgcgccg cccgctgcga
      661 tcgccaaggc cgaattgccg ccgcccgtgg gataaatcgg aatcgggtcg tacgtgttgt
      721 tgcccgtcga ggacgccgga ttgggggccg gcatgtacgg ggcataggcc ggcccgaggt
      781 acgccgggcc gctgccgtag agcttggtgt tggcgacgaa cggcggcacg tcgggcgagc
      841 gatcggccag ctcgtgggcc acgatcgacc ccagctcggg atactcggcg tcgttcaggt
      901 tcgcctgccg cgaggcgtag cccgtgagaa accggtgcgt gccgcccgaa tgctcgttcc
      961 gctcatggtg caggctgcga accagtgcga acttgtcggc cagcttcgcc agctgcggca
     1021 acagctcggt gatctcgatg cccggcacgc tggtgcggat cgggcggaac gggccgcgat
     1081 attccgccgg cgcgtcgggc ttcaggtcga acaggtcgat gtgactggga ccgccgttgg
     1141 cccagaacag gatcaccgag aaatcgcggt cgatctcccc tgccctttcc gccgcgagca
     1201 gaccggcttg cgtcggctgc acgcccagcg agagcgcccc cagcgagaga ccgccgagct
     1261 gcagccactg ccggcgattc agcggcccag tgcagctcag cggcccagtg cagcggcgct
     1321 cggcagggcc tgttttcatg cggctttacc ccaccagctc gcggatcgcc tcgcccttgt
     1381 cgatcagcgg catggggcgg tcgagggcgt cgcggaagct gaccgcgcga cagtcgatgc
     1441 ccaagtgatg atagacggtg gccgccacgt cttgcgggct caaggggcgt tcgaccacgt
     1501 gctcgccgcg ggggctggag cggccgatca cctggcccat cggcatgccg ccgcagccca
     1561 tgagcacgct gaacgtgttg ccccagtggt cgcgaccggc attcgagttc attcgcggcg
     1621 tacggccgaa ctcgcccatc gccacgacga gcgtcttttc atacagcccg cgctcgacga
     1681 ggtcctcgat cagcgcggcg atcgcccggt ccagcggtgg aatgaggagc cgcgcgcctt
     1741 cctcggtctt gtactgcccc ggagagccgt gatgatccca gggcacgcaa ttgaccgtga
     1801 caaacgtcac gccgtgctcg accagccggc gtgccaggag cgcgctctgg ccgaacgtgt
     1861 gccggccgta gcgatcgcgg agcttgtcgc tctcgcgacc gatttcgaag gcgctcttca
     1921 cctctgggcc ggtgagcagg tcgaaggctg cctgctgatg gtcgtcgagc tcgcggccgc
     1981 cccgctcgcc ggccgtcttc agccggtcga actcctcgcg gagcgtccgc cgctcggcga
     2041 cgcgctccag ggtcaagtcg ccctgcagcg tcaggttgcg gacggcgaag cctttttcat
     2101 tcgggtccga gttgacgatg aacggattcc agccgccgcc cagataagcc gcgtagtgga
     2161 acaggttgtc ggtgccgccg cggaggtgcg gcacggccac gtaaggaggc agccccggct
     2221 tattcggccc gcggagcttc gccgccgcgc agcccagggc cggccgccgc tggtcgcggt
     2281 tgtcgggcgc gttgaacgcc gggccttcga agcccgtcag catccagtgg ttgcccttgg
     2341 tatggtcgcc ggtgccgtgg ttcacagtcc gcagcaccgc cagcttgtcc atcaggcggg
     2401 cctgctcggg catcagctcg ctgaactgca cgccggcaac gttcgtgtcg atcgccttca
     2461 gcgggccgcg atattcggcc ggggcattcg gcttgggatc ccaggtctcc atgtgaccgg
     2521 ggccgccgct gagccagatc aggatcacgc tcgtaccggc tgccttcggg cgaaccgcgc
     2581 cggccgcctt caggcgggag agctcggcca gcgtcaagcc gccgacgccc agcgccccgg
     2641 cctgcaaaaa gccgcgccgc gaccagcggc cgcgatcgcc agtcctgacg tgcagcatgc
     2701 agtgtgcctc cgtttcgatc aacatatccc gatgcttagc ggggagcaag caagacaggt
     2761 gacctagtgc tggtggctgg tggctagtga ggagccccgg cactagccac cagcactaac
     2821 cactagcccc tttcactcgc gaacctacac acgctgtccg cgtccttgac aactaccgat
     2881 ttcctgttga aatagcagca gacgtttgcc accgccgccc ccttcgtcta gcggcccagg
     2941 accccgggtt ctcagtccgg taacaggggt tcaaatcccc tagggggtac tt
//
LOCUS       DASRJB010000384         5207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2364547, whole genome shotgun sequence.
ACCESSION   DASRJB010000384 DASRJB010000000
VERSION     DASRJB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364547"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="malQ"
                     /locus_tag="VFB80_10110"
     CDS             <1..1438
                     /gene="malQ"
                     /locus_tag="VFB80_10110"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZN34164.1"
                     /translation="RAGQGVWQVLPLGPTGYGDSPYQCFSAFAGNPLLVDLRDLAARG
                     WLTADELAEAPRFPEAAVDFERVKPWRIGLLRRAFERFGLSAEPEPRNDWDKFCIENE
                     WWLSDFALYMAIKREQGEVEWTRWPAELAARQPEAIAQARQRLRDEIDFQTFVQWQFF
                     RQWQALKAHANAAGVRIVGDVPIFVAHDSTDVWASRELFKLDERGLPAVVAGVPPDYF
                     SSTGQHWGNPIYDWDRQAVRGYDWWVRRLRQAMTMFDLVRLDHFRGFVAHWEIPADAP
                     TAASGRWMTGPGAALFAAATSQLGPLPLIAEDLGVITPAVVSLREEFGYPGMRILQFA
                     FGDDPEAPNYLPHHYVPDCVVYTGTHDNDTAVGWFHSRAGEGTTRSAEQIARERDYAL
                     AYVNTDGREIHWDLIRLALASVARLAVFPLQDVLGLGTEARMNLPSTTSGNWRWRLAP
                     GQFTAEHEQRLRKLAETYDRGMFATGGS"
     gene            1442..4738
                     /gene="treS"
                     /locus_tag="VFB80_10115"
     CDS             1442..4738
                     /gene="treS"
                     /locus_tag="VFB80_10115"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZN34165.1"
                     /translation="MSDPLWYKDAVIYQLHVRSFFDSIGDGIGDFDGLTQKLDYLQDL
                     GVTALWLMPFYPSPLRDDGYDIADYQNIHPQYGTLESFRRLLEAAHERDLQVVTELVI
                     NHTSDQHPWFQRARRSPPGSKERDFYVWSDTPEKYRDARIIFKDFEPSNWTYDNVAKA
                     YYWHRFYSHQPDLNYDNVAVHEAILPLVDFWFGLGVDGLRLDAVPYLYEREGTNCENL
                     PETHAFLKKLRAHVDAKFSGRMLLAEANQWPEDAVAYFGSGDECHMAFHFPVMPRLFM
                     AIHREDRFPLVDIMDQTPAIPANCQWAMFLRNHDELTLEMVTDEERDYMYRAYAAETR
                     ARINLGIRRRLAPLLSNNRRRIELMNGLLFSLPGTPVIYYGDEIGMGDNIYLGDRNGV
                     RTPMQWSADRNAGFSRANPQKLYLPIIIDPEYHYEALNVEAQQNNRHSLLWWMKRLID
                     ARKRSLALSRGSLEFLHPKNRKVLAFLRRHGDETVLVVANLSRFVQYADLDLSALAGQ
                     LPIEFFGGTRFPPLGKTPYFLSLAPHSFLWFRLLPQPSEPSPVPAHAAAPQLPVLAGR
                     QSWLDAEHRDEDDLEQTLAAFLSRGAAGDQAVKTVRITDSIRVGQGSRPSQLVLLEAD
                     RLPGRIERLALPLGLAAGSRADEIATRHSAAAIARTTDGSESSVLYDATAGGDFAAAL
                     VELIAQGQRLKTRQGAEVAGWRTVEFAPEPAAATQIKQRQSNSLFNFGNRLLLKVFRQ
                     IEEGLHPEIEFGQFFNRSGRSLPVARLLGAVEWRAKKSGPVALAVLHEYLQNEGDAWH
                     YTLDALGDFFERVVTLEPALPAPPPPDWREGSPAGAQAPGQLLEDFLATARLLGRRTA
                     ELHLALAADAEDPAFAPEPFSPFYQRSIYQTMRAHALETLDELAQKLPALDEAQRPAA
                     EQLLSQRGRIVQEFRQVLAERLETVRIRCHGDYHLGQILFTGKDFVVVDFEGNADVPL
                     SERRIKRSPLRDVASLIRSFDYTARYALAQVAAPSRRTPGALRPSDLPRVAPWTRYWS
                     GAVIAALWEAYREQPGIAALLPGTDRGCRVLLEMFLLERSLIDLGHELRERPEFINIA
                     LASVLELISR"
BASE COUNT          853 a         1856 c         1659 g          839 t
ORIGIN      
        1 ccgcgccggc cagggcgtct ggcaggtgct gccgctgggg ccgactggct acggcgactc
       61 gccctatcag tgcttctcgg cgttcgccgg caacccgctg ctggtcgatt tgcgcgacct
      121 ggccgcccgc ggctggctca ccgccgacga gctggccgaa gcccctcgct ttcccgaggc
      181 cgcagtcgat ttcgagcgcg tcaagccgtg gcgaatcggg ctgctgcgcc gcgcgttcga
      241 gcgattcggg ctatccgccg aacccgagcc ccgcaacgat tgggataaat tctgcatcga
      301 gaacgaatgg tggctcagcg atttcgccct gtacatggcc atcaagcggg agcagggcga
      361 agtcgagtgg acccgctggc cggcggagct cgcggcccga cagccggaag cgattgccca
      421 ggcccgccag cggctgcggg acgagatcga ttttcagacg ttcgtgcagt ggcaattctt
      481 ccggcagtgg caggctctga aagcccacgc caacgccgcc ggcgtgcgga ttgtcggcga
      541 cgtgccgatc ttcgtggccc acgacagcac cgatgtctgg gcgagccgcg agctgttcaa
      601 gctcgacgag cgaggcttgc cggccgtcgt cgccggcgtg ccgccggact atttcagctc
      661 cacgggccag cattggggga atccaattta cgattgggac cgccaggccg tccgcggcta
      721 cgactggtgg gttcgccgcc tgcggcaagc catgacgatg ttcgatctgg tccggctgga
      781 ccatttccgc ggcttcgtcg cccactggga aattcctgcg gacgccccga ccgccgcctc
      841 cggccgctgg atgacggggc cgggcgcggc gctgtttgcc gccgcgacct cgcagctcgg
      901 cccgctcccg ttgatcgccg aagacctcgg tgtcatcacg cccgccgtgg tttccctccg
      961 cgaagagttc ggctaccccg gaatgcgaat cctgcaattc gccttcggcg atgatcccga
     1021 agccccgaac tacctgccgc accattacgt cccggattgc gtcgtctata ccggcacgca
     1081 cgacaacgac acggccgtcg gctggtttca cagccgggcc ggcgagggga cgacgcgctc
     1141 ggccgagcag atcgcccgcg agcgcgatta cgcgctggca tacgtgaata cggacggccg
     1201 cgagatccac tgggacttga ttcgcctggc cctggcgtct gtcgcccgcc tggcggtgtt
     1261 tccgctccag gacgtgctgg gcctgggaac ggaggcccgc atgaacttgc ccagcacgac
     1321 cagcggcaac tggcgctggc ggctcgcgcc cgggcagttc acggccgagc acgagcagcg
     1381 actgcggaag ctggcggaga catacgatcg cgggatgttt gcaacagggg ggagttaagg
     1441 cgtgtccgat ccgctctggt acaaagacgc cgttatttac cagctgcacg tccgctcgtt
     1501 cttcgacagc attggcgacg gcatcggcga cttcgatggg ctgacgcaga agctcgacta
     1561 cctgcaggac ctgggcgtca ccgcgctgtg gctgatgccc ttctatcctt cccccctgcg
     1621 cgacgacggc tatgacatcg ccgattatca gaacatccac ccgcaatacg gcacgctgga
     1681 gtccttccgg cgcctcctgg aagccgccca cgagcgcgac ttgcaggtcg tcaccgagct
     1741 ggtcatcaac cacacctcgg accagcatcc ctggttccag cgggcccgcc gctcgccgcc
     1801 gggcagcaaa gagcgcgact tctacgtctg gagcgacacg cccgagaagt accgcgacgc
     1861 ccgcatcatc ttcaaggact tcgaaccctc gaactggacg tacgacaacg ttgccaaggc
     1921 ctattactgg caccgcttct attctcacca gcccgatctc aactacgaca acgtcgccgt
     1981 ccacgaggcg atcctgccgc tcgtcgattt ctggttcggc ctgggagttg acggcctgcg
     2041 gctcgacgcc gtgccctacc tgtacgagcg cgagggcacc aactgcgaga acctgcccga
     2101 gacccacgcc ttcctcaaaa agctgcgggc ccacgtcgat gcgaagttca gcggccgcat
     2161 gctcctggcc gaggctaacc agtggcccga ggacgccgtc gcctatttcg gcagcggcga
     2221 cgaatgccac atggcgtttc actttccggt gatgccccgg ctgttcatgg cgatccaccg
     2281 cgaggaccgc tttccgctcg tggacatcat ggaccagacg ccggcaatcc cggcgaactg
     2341 ccagtgggcc atgttcctcc gcaaccacga cgagctgacg ctggagatgg tcaccgacga
     2401 ggagcgcgac tatatgtacc gggcctacgc ggccgaaacg cgggcccgca tcaatctggg
     2461 catccgccgc cgcctcgcgc cgctcctctc gaacaaccgc cgccgcatcg agctgatgaa
     2521 cggcctcttg ttctcgctgc cgggcacgcc ggtcatctac tacggcgacg agatcggcat
     2581 gggggacaac atctacctcg gcgaccgcaa cggcgtccgc acgccgatgc aatggagcgc
     2641 cgaccgcaac gccggcttct cgcgggccaa tccgcaaaag ctctacctgc cgatcatcat
     2701 cgaccccgag taccactacg aagccctcaa cgtcgaggcc cagcagaaca accgccactc
     2761 gctgctctgg tggatgaagc ggctgatcga cgcccgtaag cgttcgctgg ccctcagccg
     2821 cggatcgctc gagttcctgc acccgaaaaa ccgcaaggtg ctggcgttct tgcggcggca
     2881 cggggacgag acggtgctgg tcgtggcgaa cctttcgcgg ttcgtgcagt acgcggacct
     2941 cgacctgtcg gcgctggccg ggcagctgcc gattgagttc ttcggcggca cgcggtttcc
     3001 gccgctgggc aagacgccgt actttctgtc gctcgcgccg cattcgttcc tctggttccg
     3061 cctgcttccg cagccgagcg agccgtcgcc ggttcccgcc cacgccgccg ccccgcaact
     3121 acccgtgctc gccggccgtc agtcgtggct cgacgcggag caccgggacg aagacgacct
     3181 cgagcagacc ctggccgcgt tcctctcgcg cggagcggcg ggcgaccagg ccgtgaagac
     3241 cgtgcggatc accgactcca tccgcgtcgg ccagggcagc cgaccctcgc agctcgtgct
     3301 cctcgaagcc gaccgcctgc cggggcgaat cgagcgcctg gcgctgcccc tggggctggc
     3361 tgccggcagc cgggccgacg aaatcgccac gcggcactcg gccgccgcca ttgcccggac
     3421 gaccgacggt tccgagagca gcgtgctgta cgatgcgacg gcaggcggcg actttgcggc
     3481 ggcactcgtg gaactgatcg cccaggggca gcggctgaag acgcggcaag gggccgaagt
     3541 cgccggctgg cggaccgtgg agttcgcgcc ggagccggcc gccgccaccc aaatcaagca
     3601 gcggcaatcc aattcgttat tcaacttcgg caaccgattg ctgctcaagg tctttcgcca
     3661 gatcgaggaa ggcctgcatc ccgaaatcga gttcgggcag ttcttcaacc gctccgggcg
     3721 gagcctgccc gtggccaggc tgctgggcgc ggtcgagtgg cgggccaaga agagcggccc
     3781 cgtcgcgctg gccgtgctgc acgagtatct ccagaacgaa ggggacgcct ggcattacac
     3841 gctcgacgcc ctgggggact tcttcgagcg cgtcgtgacg ctcgagccgg ccctgccggc
     3901 acctccgccg cccgattggc gcgaagggag cccagccggc gcgcaggctc ccggccagct
     3961 gctcgaggac ttcctggcca cggcccggct gctgggccgc cgcaccgcgg agctgcacct
     4021 ggcgctggcc gccgacgccg aggacccggc cttcgcgccg gagccgttct cgcccttcta
     4081 tcagcgctcg atctatcaga cgatgcgggc ccacgcgctg gagacgctcg acgagctcgc
     4141 ccaaaagctg cccgcgctcg acgaggccca gcggccggcc gccgagcagc tgctttccca
     4201 gcgcggccgc atcgtccagg aattccggca ggtgctggcc gagcggctgg agaccgtccg
     4261 catccgctgc cacggtgatt accacctggg ccagatcctg ttcaccggca aggactttgt
     4321 cgtcgtcgat ttcgagggga acgccgacgt gccgctcagc gagcggcgga tcaagcgctc
     4381 gccgcttcgc gacgtggcct cgctgatccg ctcgttcgac tacaccgccc gctacgcgct
     4441 ggcccaggtc gccgcgccga gcaggcggac gcccggcgcg ctgcgaccca gcgacctgcc
     4501 ccgcgtcgcc ccctggaccc gctactggtc cggcgcggtc attgccgccc tctgggaggc
     4561 ctaccgcgag cagcccggca tcgccgccct cctgcccggc accgaccgcg gctgccgcgt
     4621 gctcctcgag atgttcctgc tggagcggag cctcatcgac ctgggccacg agctccgcga
     4681 gcggcccgaa ttcatcaaca tcgccctggc cagcgtgctg gagctcatca gccgctaagc
     4741 cgccagtgtt tcaaagccgc gaccagtggt cgcgctctac tcgtgcgccc gtcggctgcc
     4801 cttaagggcc gcggcgagta taacgaaaac tgccgcgagc acagattcgc gtcgcgcctc
     4861 attgtgctgc cgctcgcgcc gcaactgcct gacacgcgag gacttccggc gtatggccgg
     4921 gcgattttcc ggcataagga acataagcgt tagagagccg gcggggagtt ttcgttatat
     4981 tctgccgccg agcggagcgg cggcgattcc gccacagttg ccaacgacca aattgagaga
     5041 gagcgggtgc catttgcggc cgagcagcca aaacggcagt ccgccgcgcc aaagggaact
     5101 atactgcaaa tagcgtacca atgttcagag tcctgcactc cgccccgccc cgtgccgcaa
     5161 cgcggcaacc ttcgtcagcc ctgggacaga gaagcgctgc ccaggga
//
LOCUS       DASRJB010000385         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3489768, whole genome shotgun sequence.
ACCESSION   DASRJB010000385 DASRJB010000000
VERSION     DASRJB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3489768"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFB80_10120"
     CDS             complement(<1..1326)
                     /locus_tag="VFB80_10120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN34166.1"
                     /translation="MKPRREKHHRAADQVDWPGLLRSGVLAGAVALVVVATLIPSEGS
                     VATGSYAILAAGWCVLAIVWCLSLLVDKQPGLELGWTELAAAALIAWHTLAGMVVLGS
                     GNGRYALNSVWLYIGYGLTAWILRQTLRTAAQVRALVAAGLWLALLLASLGFYQYFYA
                     FPQQRRQYEANRERILAENDIPLDQPSQVAQFENRLRSVEPLGTFALTNSLAGLLAPW
                     LCAALAVALAVRAVPQQRRTLLAALAFAVVLAACLVLTKSRTAYLATAAGVVLVGLYC
                     RPSGWRLDWRIPAAAGGMLVVLGLVAVYLGGLDAQVLSEAPKSVLYRLEYWRATAALI
                     ADHPLFGCGPGNFKSVYAEYKLPQASETISEPHNFLLEVWATSGTPGVVLLLLMVVAF
                     AVDLSRGLRQAPSASTDSGDEAKTARRTIFGGALAGLLLAMPLALADDSP"
     gene            complement(1323..2459)
                     /locus_tag="VFB80_10125"
     CDS             complement(1323..2459)
                     /locus_tag="VFB80_10125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZN34167.1"
                     /translation="MAPRALFLFIAGSVLPSLIVSLLATWLVRANAARWGLIDLPGER
                     KVHTAPTPRGGGLAIWLGVVATFAAAQLFLLVAAKSPAVNELIPEFARGHVAGIWSQS
                     GKLWILLGGGTALALLGLADDRGGLSWQLRLAVEFAVAATCVWLVPNLRLTAFLPYPI
                     LTGALSVLWIVALINAFNMLDNMDGLSAGVAAIASSMLAAVLLLAPDPETRGPQLFVA
                     GLLLVVVGALAGFLWHNRPPAKIFMGDSGAYFLGFMIAAATLLASYTGYQGQRSHAIL
                     APVLVMAVPLYDMTTVILIRLMAGKSPFHADKNHFSHRLVDLGLTKPQAVLTVYLTTA
                     TCGLAALLLHQVERSGAVLIVLLVGCVLSLIAILESTARRTIKK"
     gene            complement(2462..2992)
                     /locus_tag="VFB80_10130"
     CDS             complement(2462..2992)
                     /locus_tag="VFB80_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HZN34168.1"
                     /translation="MADIQPHRPVLLVLAAFSRHAEALDWARQTAEEAWGPVALQSDR
                     FDHRETDYYEPTMGEGLSKSFFAFEPLVDPADLVACKETSNQWERDYQQLGRHSEQRP
                     LNLDPGYLTEAKLVLATTKDRDHRLYLDRGIFAEVTLHFQHGRWQPRPWTYPDYQRAD
                     YHEFFSRCREFLRQRT"
     gene            complement(3002..>3288)
                     /locus_tag="VFB80_10135"
     CDS             complement(3002..>3288)
                     /locus_tag="VFB80_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN34169.1"
                     /translation="EAGADDLKREGDGFQVICPPDAFQAVGDALAAAKIDPDSKQLSR
                     VPTNTVEIKDVATARTVLGLIEALDDHDDVQNVAANVTIPDEALAAIAAG"
BASE COUNT          605 a         1078 c         1135 g          470 t
ORIGIN      
        1 cggagagtcg tcggcgagcg ccagcggcat cgccagcaaa aggccagcga gcgctccgcc
       61 gaagatcgtg cggcgcgcgg tctttgcctc gtcacctgaa tctgtcgagg cggaaggagc
      121 ctgccggagg ccccgcgata agtcgacggc gaaggccacg accatcaaga gcaggagcac
      181 gacgcccggc gtgccactcg tagcccacac ttccagcaga aaattgtgcg gttcggagat
      241 tgtctcgctg gcctggggga gcttgtactc ggcgtagacc gacttgaagt tgcctggacc
      301 gcagccgaag agcgggtgat cggcgatcag ggcggccgtc gcgcgccagt attccaggcg
      361 gtagaggacc gacttgggcg cttcgctgag gacctgggca tcgaggccgc cgagataaac
      421 ggccacgagc cccagcacga caagcatgcc gccggccgcc gcaggaattc gccaatccag
      481 ccgccagccg gaaggccggc aatagagtcc gaccagcacg acgccggcag ccgtcgccag
      541 gtacgccgtg cggctcttgg tcagaacgag acaggcggcg aggacaacgg caaacgccag
      601 ggcggccagc agcgtccgcc gctgctgcgg cacggcgcgg acagcgagcg cgacagccaa
      661 cgcggcgcac aaccacggcg cgagcaggcc cgccagtgag ttagtcagcg cgaacgtccc
      721 cagcggctcg acgctgcgga ggcggttctc gaactgcgcc acctgcgaag gctggtcgag
      781 ggggatgtca ttctcggcca ggattctctc gcggttggcc tcgtactgcc gccgctgctg
      841 cgggaaggcg taaaagtact gatagaagcc gaggcttgcc agcagcagcg cgagccacaa
      901 tcccgcggca acgagggccc ggacttgcgc ggccgtgcgc agcgtctggc ggagaatcca
      961 ggccgtcagc ccgtagccga tgtagagcca gacggaattc aaggcatagc gaccgttgcc
     1021 gctgcccagg accaccattc ccgccagcgt gtgccaggcg atcagcgccg ccgcggccaa
     1081 ttcggtccag cccagttcca ggcccggctg tttatcgacc aaaagcgaga ggcaccacac
     1141 gatcgcgagg acgcaccagc cggcggcaag aatcgcgtag ctgccggtgg cgaccgagcc
     1201 ttcgctggga atcagcgtcg ccacgacaac gagggccact gcgcccgcga gcacgccgct
     1261 gcgcagcagg ccgggccaat cgacctggtc ggcggcacgg tggtgttttt cgcggcgggg
     1321 cttcatttct tgatcgtccg ccgggcggtg gattcgagaa tggcgatcag cgacagcacg
     1381 cagccgacga gcagcacgat cagcaccgcg ccgctccgct cgacctggtg caggagcagc
     1441 gccgccaggc cgcaggtggc ggttgtgaga tagaccgtga gcacggcctg cggcttcgtg
     1501 agtcccagat cgacgagccg gtgcgagaag tggttcttgt cggcgtgaaa cgggcttttg
     1561 cccgccatca agcggatcag gattaccgtt gtcatgtcgt agagcgggac cgccatgacc
     1621 agcaccggcg cgaggatcgc atgcgaccgc tggccctgat agccggtgta gctcgccagc
     1681 agcgttgccg cagcgatcat gaagccgagg aaataggccc ctgaatcgcc catgaagatt
     1741 ttggccggcg gccggttgtg ccacaaaaag ccggccagag cgccgacgac gacgagcagc
     1801 agcccggcga cgaacaactg cggcccgcgg gtctcgggat cgggggcgag cagcagcacc
     1861 gcagcgagca tgctcgaggc gattgccgcc acgccggcag aaaggccatc catgttgtcg
     1921 agcatattga aggcgttgat gagcgctacg atccacagga cggagagcgc tcccgtcagg
     1981 atcgggtacg gcagaaacgc cgtcagccgc aggttcggaa cgagccagac gcaggtcgct
     2041 gccacggcga attcgacggc caggcgcagc tgccaggaga gcccgccgcg gtcgtcggcg
     2101 aggccgagca gcgccagggc cgttccgccg ccgagcagaa tccacagctt gcccgactgg
     2161 ctccagattc ccgccacgtg tccgcgggcg aactcaggaa tcagctcatt gacggcgggc
     2221 gacttcgcgg cgacgagcag aaacagctgt gccgcggcga acgtcgcgac cacgcccagc
     2281 cagatcgcca gcccgccgcc gcggggcgtc ggcgcggtgt gcaccttgcg ctcgcccggc
     2341 aggtcgatca gcccccaccg cgccgcgttg gcccgcacca gccacgtcgc cagaagtgaa
     2401 acgatcaggc tcggcagaac cgacccggcg atgaagagga agagcgcgcg tggggccatg
     2461 actaggttcg ctgccgcaag aactcgcgac agcggctgaa gaattcatga tagtcggccc
     2521 gctgatagtc gggataggtc cagggccgag gctgccagcg gccgtgctgg aagtgcaacg
     2581 tgacctcggc aaaaatcccg cggtccaggt acagccggtg gtcgcggtcc ttggtcgtgg
     2641 ccagcaccag cttggcttcg gtgagatagc cggggtcgag gttcagcggc cgttgctcgc
     2701 tgtgccggcc gagctgctgg tagtcgcgct cccactggtt gctggtctct ttgcaggcga
     2761 ccaggtccgc cggatcgacg agcggctcga aggcgaagaa cgacttcgag agcccctcgc
     2821 ccatcgtggg ctcgtagtaa tccgtctcgc gatggtcgaa ccggtcgctc tgcagggcca
     2881 ccggccccca ggcctcttcg gcggtctggc gggcccaatc gagcgcctcc gcgtggcgac
     2941 tgaacgcggc gagcacgagc aagaccgggc ggtgcggctg gatgtcggcc atgcgtccag
     3001 tctaacctgc cgcgatcgcc gccaaggcct catccggaat cgtcacattg gccgcgacgt
     3061 tctgcacgtc gtcgtgatcg tcgagggcct cgatcaggcc cagcaccgtg cgggccgtgg
     3121 cgacatcctt gatctcgacg gtgttggtcg gcacgcggct gagctgcttg ctgtcggggt
     3181 cgattttggc ggcggccagc gcatcgccga ctgcctgaaa tgcgtcgggc gggcaaatga
     3241 cctggaaacc gtccccttcg cgcttcaaat cgtcggcgcc ggcctcca
//
LOCUS       DASRJB010000386        21506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_391267, whole genome shotgun sequence.
ACCESSION   DASRJB010000386 DASRJB010000000
VERSION     DASRJB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391267"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFB80_10140"
     CDS             complement(<1..229)
                     /locus_tag="VFB80_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34170.1"
                     /translation="MKAAIQQAIFSAAGRHWWHGRFSSAPTGLFSITVAQRTSAAWNF
                     LLIENGPLAHASGRALSLLRSHWRNASATRRE"
     gene            complement(226..1656)
                     /locus_tag="VFB80_10145"
     CDS             complement(226..1656)
                     /locus_tag="VFB80_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN34171.1"
                     /translation="MPRRYPPGPINFNASFGLTWRHAYSLCRDPLGFVSWAAETYGDV
                     VFYRVFNYVAYQVNHPDLIREVLVTKARSFLKADRQMNIIRQVAGDGLLSAEGELWMR
                     QRRIMLPAFQTAAAQRMAEIGLKLAQSMMDDWEPGDEIDIDEAFITLLIRIVSEAFFA
                     VETDREARWLGEQMSTVSRAMLDQISSVASLPPWVPFGTRWHQQRAQTALYRYVDEAI
                     VHRRSDQTRSRDLLSVLLTAVDEEQGQRGMSDTQARCEAMTMFFAGHHTSAAALTWTV
                     YLLSENPGVYETLLAEIDQVLEGRPPELNDIPRLPYTEMVLKESMRIYPPAWALFSRQ
                     TVEEVELGGYELPRGAWVFIYPWVTQRDPRVFPDPLRFDPERFAPQRASAIPTGAYFP
                     FGLGAHSCIGTRMAMTTLMLVLPAVLQRFKYRQAPGQRPPIPEPLVSIRPKRGLRMIV
                     EARPNAVPVGGTGVSPVPNEAAIVRA"
     gene            2154..3110
                     /locus_tag="VFB80_10150"
     CDS             2154..3110
                     /locus_tag="VFB80_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN34172.1"
                     /translation="MSERKLTIGGSILWAMLVVVAGCFEGEPKKGTVGVSVLTVTNPF
                     FRVIGDNITAELKKAGYETVVVSGEDLPTQQRQVRDFISAGYAAIVLCPCDSSGIGPV
                     IEEANAKGIPVFTADIACTAANAKVVSHIATDNLAGGKQAAEAMIEALGEAGGKIAIL
                     DYKDVESCQLRVQGFKEVLAAHNAKAGAGQIEIVAELPGNGDTAKGYKAAEDALQAHP
                     DLVGIFAINDPSALGARAALEKAGKAEQITLIGFDGQLPGKRAIRDGKIYADPIQHPD
                     QIGVTTARSILAHFAGEPVEKFQLIPTALYRQADAKNDPELK"
     gene            3217..4530
                     /locus_tag="VFB80_10155"
     CDS             3217..4530
                     /locus_tag="VFB80_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN34173.1"
                     /translation="MLSKILREHTTLLVLLLLGAALSVATLAEQHPTGASGGKQLARR
                     ILAQSPRPQRVLIVVGGTPEDAQFAAAAEEMLAAGGASVAVVRGQPADARQAIDQAIA
                     AGRPPQAIGATNVTRAWGVFDKLGQRWPALAGARVVAPASYWWPNFLKTANLVNVVNQ
                     VSDIAIVAIGMTMVIIAGGIDLSVGSLIALAAIIMTKLIRDYCGGVEAGAAAVVLCAA
                     AAIAASGAIGGCTGLLVTRARIPPFIVTLGVMSIASGAASIVSRGETIDQVPRAIGWL
                     GRGADLGVPNTTVLMLLLYVIADVVMKRTTFGRYLYAVGGNRQAAWLSGVPVDRVVTL
                     SYVISAALAALAGVMQVSLFHSGKPTFGKGYELQVIAAVVVGGTSLAGGEGKMFGTLL
                     GALIIAVVQNGMNLLGLGGEWQPVVLGAVILAAALIDRLKRRREE"
     gene            4638..6248
                     /locus_tag="VFB80_10160"
     CDS             4638..6248
                     /locus_tag="VFB80_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN34174.1"
                     /translation="MARFVSFLILVGILIFLTIIFYQVMSGFFVPLFVAALLGVVVQP
                     LYRWILAQCRGYRYVAAGITTGLVALIVLLPIGVVIATASLEGLSLVDQLQLANVRTK
                     LDELRRDLGLQIPREEDVRTIEAKLTRWRNLQLKGESLDIKSEEVNNLLQRVDELEKW
                     IKEQQQQTGFARADVSALKANLEALHKAETGSVEADNALQTAAAEFREFKRNLLGGTY
                     KAWLAEWANPTDEQVEQLRRTIMTTAGPVLSLGGDTIVLIVKLIAGLLIMMVALFFML
                     AEGARMLDGLVRVSPLNEQHVRELVAEFDRACRAIVSATLLSALAQGVLAGVGFYVCG
                     LRGSVALLMLLTMVLALVPFTGAAAVWIPVSLYLYFYQGATWAAVGLAIYGAIIISGA
                     DNVIKPLVLHGQSNLHPLLALLSVIGGIKALGPVGILVGPMVVVFLQVLMRLVHRELS
                     QMDRSNWMFWRGFGGAEPPPAAAAAAAAPAAAAASTGAAPAEPATTKTVPPAPPPAAS
                     TPPADGNGHHQPGQPGKQHQHHGKKRRR"
     gene            6372..7709
                     /locus_tag="VFB80_10165"
     CDS             6372..7709
                     /locus_tag="VFB80_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34175.1"
                     /translation="MNAVALMVALAAAGVDYQVETTSDKQQQYTIQIEPELLLRVAGG
                     EEIHSEVPADAGPLQRLCIRIGMTKPVHAPTSEAAFRQLLLSASRWAMADRTLTAADS
                     PPTILWPARSTPEQSRNLRYGWQPDAEGKQSYYVQIDLAMLRSLAAGDEIYAPIDPSA
                     GRLDRFIVTMTQKDYPPRIAGAAQAPAFVPTGPPFQTGGQTSINLLPTDNPRSQFQTP
                     PATGGGFTPNNTALTPVEIPPAPGGPVAPRDYSWPMPRPNPQATMPLASVPPATVQPQ
                     PAPVVDNRPGAFSPPATNTYSTAPPNNYPANNMLEPPRSNFGGTASQQVADSRAYPGV
                     TPNGTYGTNAAYGQQQGFGQPQGLSAADNRIAMQPPPVAATLPTVNPTGSLTSGAVPP
                     ALPAKEGWGMFVVVLFALFFSIGGNLYLAWTALEFHNRYRNAIDRLRSAARSS"
     gene            complement(7746..9524)
                     /locus_tag="VFB80_10170"
     CDS             complement(7746..9524)
                     /locus_tag="VFB80_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN34176.1"
                     /translation="MAAPDRQQQIAQAEEILGDRLSQVGFVKGLFFGQYLEGRLLPYP
                     DHARDPETTDLVERLRSFCTEKIDPVAIDRQAEIPRPVIEGLGRLGVLGACLPKDCGG
                     LGLSQTAYCRILEVLGGHCGSTALFVNAHHSIGPRALVLFGTEAQQKAYLPKLASGEW
                     ISAFALTEPEAGSDAANVQSTAMPTADGRGYTINGQKRWITNGGVAQVLTVMARTPVE
                     GSHTTVSGNEASKITAFIVTPDMPGFQVVEKRMEKMGVRGTATGRLAFNDMFVPKENL
                     LGQLGKGLKVALTVLDFGRTTFGASCTGAAKFCVARAIEHANRRVQFGQTLGNFELVK
                     EKLAHMQAGVFAMEACTYQTAALIDSGEGDFMLETAMLKVFATETLARILRDTFQLHG
                     GLAYFTDQPLERMVRDAMINTIGEGANEVLRAFTALVGMRDVGLELQSIRDAIYSPLG
                     NVTRLSRFAGRKLGSLLVSPTIPVRNSELEPEAAELGRLVNLLGSSVQRLLVTYQLGI
                     VDRQYQLGRVADAATEVYTSSCVLNRLDFLLRNSHDHDGGLRLDLQTGRHYLRTARRR
                     IKQSLAALWDNDDEATTALADRMLRS"
     gene            9697..10440
                     /locus_tag="VFB80_10175"
     CDS             9697..10440
                     /locus_tag="VFB80_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZN34177.1"
                     /translation="MPRKENIDRILRDWPYDPHGISVRKKKGDDGREVLQMRLDLGML
                     QLETAGRPDGQRPQGADSYYDYLIGRTLANGDEYVMTEDDCEEADREFVQYYHRRICW
                     LALREFQRAVLDADHTLGMMDFCKKHSPDEQWTLSHEQYRPFVLFHRTQAASLAALSD
                     KGAEAAVEEITKGLDQMRQVFVDYEAEEEFDEDELVQRLIELRESLRQHFRVGRTLQE
                     QLADAVAAEKYELAAEIRDELNRRDSRAR"
     gene            complement(10412..10627)
                     /locus_tag="VFB80_10180"
     CDS             complement(10412..10627)
                     /locus_tag="VFB80_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HZN34178.1"
                     /translation="MHWTDYLALGLFAVAAIVVAARAYRAFFGRSKAGCGSGCGSCSS
                     NSAEPKRGSLLTIGSPPDATAPLNRGG"
     gene            10789..13497
                     /locus_tag="VFB80_10185"
     CDS             10789..13497
                     /locus_tag="VFB80_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34179.1"
                     /translation="MATAFLFFLVSAPVAWGQAAAAPPTAEEIAAAIGRLGDDSFDVR
                     QKATEWLWQAGPAAETQLRAALKSTDPEVRTRAASVLDKIRFGLRPDTPPDVAILIDQ
                     FRHAGSAALRRQALTALQDKGQWAVILSLVRRETDPNERRNLATAISADAGKLLRPLL
                     AKGDFDQAEQVLELTAVNEAGLTQLATFLLLTQRLESQIEAVRQRLAADPQEDEWRRL
                     ALYLRAKGDFTAAADAAQKAGDALFAAKVLAESGRFAEAAVAAQSAPKGSGNRSDADA
                     FAAAYFDLARDETQFQRTIELLKQAANIDKIADKPQPIGPADPFGQPASSPTAASAWR
                     LAEALLIAERVEEGLAVLKKTHPVQAHALLMRQHRHHDALEFVGVTAEKKLDRAWLDS
                     LPGGLGDVNQQLSVRFGLAAQVARQLRELGRIEQTKQIAETLRAIAAAEGLNGFRWSA
                     LATLHWQLDQYDEAFAALEQALASGQQPASAFTGLARTQASLAAAWFTEITTRDPLAD
                     RKAAIAQAIWLVTAQPPAGKLPANWRDLVERSAAEARKLPQGQRGANLMLPADIIRVR
                     GDLPLARKLYEEGEEDFAAATRQAADVAAALEDWPAAAAKYGKLAGPDNSDLLVVYLH
                     GHALTKTGQEKEGKESLRLASLTALAPDVRYAIALGLQERGLKELAAEQFEIVRRTAL
                     PDSSISVNAAQRVGNLVNEEQPQRACDCWRQLHLHILNSNSNFLEAEGYLSLPQIMHK
                     VRARAALAGGKAEEVAAELERCEKLLPGDVRLTVDLTPKLDGAGLRAIADQVFERSIA
                     SHRRVLEEFPGSPTFLNNAAWICARAQRQLDEGLALAERAVKAAPDEGSYHDTLAEVH
                     FQRGDRDAAVAAARKAAELSPENKLFAKRLKHFQEDPVKTLDSVNE"
     gene            complement(13599..15533)
                     /locus_tag="VFB80_10190"
     CDS             complement(13599..15533)
                     /locus_tag="VFB80_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34180.1"
                     /translation="MHGPPTVQRGISLAIAAAIGLAALAAKAGRAQDGDWPQWRGAAH
                     DGHAPGERLADSFPPAGPPVLWAREIGQGYSGFAVSGGRVYTQSQSLYEQSVLCLDAE
                     TGRTIWSYSYGWPYDGGGLYPGPRSTPTVSGGQIYFASPQGLVGCLTTAGRLVWSVNI
                     NELFGGRGTDFGYSCSPLVVDGLVILPVGGERASVVALSTADGATVWKAGSNPASYST
                     PVPIEWRGQPLVVALLQNSLACIHRRTGELWWELPLSQGYDEHATALIYREPYLLKTG
                     PFHSGATLLELKPDGERCQPQEVWFNEKLSNDVASSVLAGETIFGFDLREAQSRLNRP
                     SRGEFRALDFLTGQIRWSSAEPGHAQIVAADGKLILFNDRGELILARASSERYEELGR
                     AAVFPGEVCWTAPALANGRLYLRTPSRAVCLYLGREPLAMSSPLSVLDAAGGARFNPG
                     VLLGGERDYPATLPQTTDFGRWYAWCSAALLCCLLASGVCLWLAPKKRAWIDAAFWTA
                     ILLCGLAGSAVLNRVQPLYVFTWPLALWAAFQLTISRSWSVRNSPLVSWRRGSSYAVG
                     LAFLGLGGLYFHLCRRLGLAIEWGFLAGFLGALPFSVLAAALAGAERRKWLLEASALF
                     ASFAAYYWSCVLFLWWRLGP"
     gene            15623..18562
                     /locus_tag="VFB80_10195"
     CDS             15623..18562
                     /locus_tag="VFB80_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34181.1"
                     /translation="MSSFSLEDKFPDLVPIQTAPSLHTVNGIGTGIYGRRDYDEETGT
                     YVTTYCWCVLFIPLFAVAAYRVADAPGGGYYFLGKTRLSGLAKAWNALLAACMIAGLG
                     GGTFCAWHYSPENVALRQLAAAEQSIAAGEHAGAAKEFEKLALGRTKQAAPARERLEK
                     WLAAPPAGAEASDYFEVFKSAAALNQMGRWPAGPQALHDRALTLAELVPAGQSPLAVS
                     LLKAAEPLDENPAASAARKRALLEKLQARAPQDLELTVLLAQALEQEGDFERCEKLLL
                     PLADKLADTEGARILGQRHLSQGKFNEAHALLAPYCKSRMAQYRAAEEAFEAAYKQAQ
                     ETEINVLERNEAFRRRYDAASDEQRQQMVAEKIDEAVKNDPQVAKQRDALRQASRVVP
                     VALDFGVVQLYRAQGLSDPAERKAELQQAETTFLSLGNAIGDTDEYKLNLGKVYYWLG
                     KSKEGRKLLDEILFRAANNFELRMAVAQTLREVGAETDSRNLVEHIYNNATSDDHKYE
                     AAELRALLFFDLDDEIAWLNRARPGDPDVKASLALARGQKGLQDGDEKAAEQNLRQAA
                     ELYTSFPESMSSLNDAALCYSGLFHSRGDTTDLRQAARLLDRAVSLKPDNSVLMINAA
                     DLYFDVALADAFATQIDLRLIHSVGRGGLLGFFYRDGAGRDEVIARLKTSPALAKATQ
                     YFDKSILLAPKRGSNYDQASALYTLLDDNAALERILRQIDDAHPDTQETVTETLKYLA
                     GDYDKKTAQTVATAQAASRKLVDHWRAAPAGDKRNRSLAAAQMAAWGALMARSASPAD
                     SPPDAEELLKLAEEAVRTHECSMTEQTLISSLLYRVHAQNVAKSPAYRQFAQKYRRHL
                     TPYETLLLALDQKPLAPAILAHADFKRAVELSKQRLARFPRAVAADDWMMLRYADPAA
                     AEQIARRLGDGTLEGRESQIDLRLHPASSHFAVLESWRLQSTGRAGEATAVFQKLNKQ
                     GVRWPQP"
     gene            complement(18559..19572)
                     /locus_tag="VFB80_10200"
     CDS             complement(18559..19572)
                     /locus_tag="VFB80_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN34182.1"
                     /translation="MIEVASAQRPLFLGFDVGGTNIKLGVVDDAGRPVCNTKIATEEE
                     QGPREAVKRARVAVDEMLATCGLNIGDLAGVGLAAPGTMDIPRGIFLQPHNLPHWHYF
                     NIRDCVAEAFGLPTVFANDANAAAYGEFWVGSGQKHHSIIMLTLGTGVGGGIIIGDLS
                     VDGENSHGSECGHIIIDSSPLARMCGCGQPGHLEAYCSATALVDRVQDLIDGGRPTSL
                     QARITPQTPLTALMIAEEADRGDSLSFECIMELGTWLGLGIVSLMHTIDPGAVILGGA
                     MNFGGHEDPVGRRFLEHVRGVVRSHAFPIPAQKTTIDFAELGGDAGYIGAAGIARLAF
                     HKR"
     gene            19709..20398
                     /gene="bshB1"
                     /locus_tag="VFB80_10205"
     CDS             19709..20398
                     /gene="bshB1"
                     /locus_tag="VFB80_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HZN34183.1"
                     /translation="MLDILVIAPHPDDAELGAAGAILKFKAEGKKVGVLDLTSGEPTP
                     FGSLEKRAAETAAATKILGLDWRQNLGLPNRSLEATLDARKQLAGVIRQTRPRWLFAP
                     YWVDAHPDHVAATQLVEGARFWSKLTKTDMPGEPWHPQRIYNYYCVHLKLAAQPAFIL
                     DISPFWEQKLAAIRCYASQFIEGRPSAAPTFLDQLRDEAAYFGKCIGVPYGEPFHCRE
                     PIGLSSMQDLV"
     gene            complement(20761..>21506)
                     /locus_tag="VFB80_10210"
     CDS             complement(20761..>21506)
                     /locus_tag="VFB80_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HZN34184.1"
                     /translation="VLAVPRPNVAVLATGNELVEPGEMPGPGQIRNSNGPLLAALVRQ
                     TGATPIDLGIARDDAADLRSKVSAGLAHDVLVLSGGVSAGVLDLVPQVLAELGVRQVF
                     HKVNLKPGKPLWFGVHPRSAGVSTLVFGLPGNPVSTLVSFELFVRPAIGVLSGRGPIG
                     LTKMRGRLSREHRQRGERPAYWPSASHIAATGCEVTPLAWKGSGDLRTLSEANALACF
                     PAGDRVFAAGETIEILLLDDGLSAGPGSG"
BASE COUNT         3770 a         7164 c         7183 g         3389 t
ORIGIN      
        1 gctcgcgccg cgtggcactg gcgtttcgcc agtgcgagcg aaggagactg agcgcgcgcc
       61 ccgaggcgtg tgccaatggg ccattttcga tcaacaggaa gttccaggcc gcgctcgttc
      121 gctgggcaac cgttatcgaa aaaaggccgg tgggagcact ggagaaacgc ccgtgccacc
      181 agtggcgccc ggccgcgctg aaaatggcct gttggatcgc ggctttcaag cgcgtacgat
      241 cgccgcttcg tttggcactg gcgacacgcc agtgccaccc accggaacag cattcggccg
      301 ggcttcgacg atcatccgga ggccgcgctt gggacggatc gagaccagcg gctccgggat
      361 cggcgggcgc tgccccggcg cctggcgata tttgaagcgc tgcaagacgg ccggcagcac
      421 cagcatcagc gtcgtcatcg ccatgcgcgt gccgatgcag gagtgcgcac ccaggccgaa
      481 gggaaagtac gcgccggtcg gaatcgcgct ggcccgctgg ggagcgaacc gctcggggtc
      541 gaaacgcagc gggtcgggaa acacgcgcgg gtcgcgctgc gtgacccagg gatagatgaa
      601 gacccacgcg ccgcgcggca gctcgtaccc gcccagctcg acttcttcga ccgtctggcg
      661 cgagaacagc gcccaggccg gcggatagat gcgcatcgac tccttcagca ccatttccgt
      721 gtagggcagc cggggaatat cgttcagctc cggcggccgg ccctccaaca cctggtcgat
      781 ctcggccagc agcgtctcgt atacgccggg attctcggag agtagataaa cggtccaggt
      841 cagcgcggcg gccgacgtgt ggtggccggc gaagaacatc gtcatcgctt cgcagcgggc
      901 ctgcgtgtcg ctcatgccgc gctggccctg ctcttcgtcc accgcggtga gcagcaccga
      961 gagcagatcc cggcttcgcg tctggtcgct gcgccggtgg acgatcgcct cgtccacgta
     1021 gcggtagagc gctgtctggg cccgctgttg atgccagcgc gtgccgaaag gcacccaggg
     1081 cggcaacgag gccacggagg agatctggtc gagcatggcc cgcgagacgg tgctcatctg
     1141 ctcgcccaac caccgggctt cgcggtcggt ctcaaccgcg aaaaacgcct cgctcacgat
     1201 ccggatcagc agcgtgatga aggcctcgtc gatgtcgatt tcatcgcccg gctcccagtc
     1261 gtccatcatc gactgcgcga gcttcaggcc gatctcggcc atccgctggg ccgcggcggt
     1321 ctgaaacgcc ggcagcatga ttcgccgctg ccgcatccag agctcgcctt cggccgagag
     1381 cagcccgtcg ccggcgacct gccggatgat gttcatctgc cggtctgcct tcaggaagct
     1441 gcgggccttg gtcaccagca cttcgcggat caggtcgggg tggttgacct ggtaggcgac
     1501 gtagttgaag acccggtaga agacgacgtc gccgtaagtt tcggcggccc agctaacgaa
     1561 gcccagcggg tcgcggcaca gcgaataggc atgccgccac gtgagcccga agctcgcgtt
     1621 gaagttgatt ggcccaggcg gatagcggcg cggcatagcg tggcacgcgt cccgtgaatg
     1681 ggaacctccg tgcctgcggg ccgtcgtgta ttgaaaggat aggtcgcaga atggctttcc
     1741 cctgtcggag aaaaagacga agcgtctggg aataaacgga acggcccgcg catctataca
     1801 tttgtgtcca gtgcgctcgc gcggcggctt gacgagtggg ggttatttcg atgtttctta
     1861 cacggatcgc gttgcagcgg agattgggaa tcggttgtct ctgactcgcg tgcgcgccct
     1921 gttcagcggc tcatttttgg gtctctaacg cttcaaaccc taatgtaaat gcagctcgtg
     1981 actattcaac ttaagttgaa atcggcaaat ggtttatggc gcatagatct agcaacacta
     2041 gggtttcgcg gcccacagac catccacccg aaagaggact gttcagcctt cgcgactgaa
     2101 caggggctag gatgacgccc agcgtcacaa acccaagcga aagggcgacg gccatgagcg
     2161 agcggaagct caccatcggg ggctcgattc tttgggcgat gctggtcgtt gtcgcggggt
     2221 gcttcgaggg ggagccgaaa aaaggcaccg tcggtgtttc cgtgctgacg gtgaccaacc
     2281 ccttcttccg cgtcatcggc gacaacatca cggcggagct gaagaaggcc ggctatgaga
     2341 cggtggtcgt cagcggcgag gacttgccga cgcagcagcg gcaggtgcgg gacttcatct
     2401 cggccggcta tgcggcgatc gtcctttgcc cgtgcgacag cagcggcatc ggcccggtga
     2461 tcgaagaggc caacgccaag gggataccgg tcttcacggc cgacatcgcc tgcacggccg
     2521 cgaatgccaa ggtcgtctcg cacatcgcca ccgacaatct cgcgggcggc aagcaggcgg
     2581 ccgaggcgat gatcgaagcc ctcggcgagg ccggcgggaa gatcgccatc ctggactaca
     2641 aggacgtcga gtcgtgccag ctgcgggtgc agggattcaa ggaggtgctc gccgcgcaca
     2701 acgccaaagc cggagccggc cagatcgaga tcgtcgccga attgcccggc aacggcgata
     2761 cggccaaggg ctataaggcg gcggaggacg ccctccaggc ccatcccgac ctcgtcggca
     2821 tcttcgccat caacgacccg tcggcgctcg gcgcgcgggc cgccctcgaa aaagccggca
     2881 aggccgagca gatcacgctg atcggcttcg acggacaact gcccggcaag cgggccatcc
     2941 gcgacggcaa gatctacgcc gacccgattc agcaccccga ccagatcggc gtcaccaccg
     3001 cccgctcgat cctggcccat ttcgccggcg agccggtcga gaagttccag ctcattccga
     3061 ccgcgctgta tcggcaggcg gatgcgaaga acgatccgga attgaagtga cagggcggtt
     3121 ggtgtttaga tgtcgtttaa cccggagccg gaggcgacgg cggctacccg cgagtaaact
     3181 gcgcctccgg ctacgggcta aacgagcaat acgaccgtgc tttccaagat tctgcgcgag
     3241 cacacgacgc tgctggtgct gttgctgctc ggcgcggcgc tctcggtcgc gacgctggcc
     3301 gagcagcatc cgacgggcgc gagcggcgga aagcagctcg ctcgccggat cctggcgcag
     3361 tcgcccaggc cgcagcgggt gctgatcgtc gtcggcggca cgcccgagga cgcgcagttt
     3421 gccgcagccg cggaagagat gctggcggcg ggcggcgcga gcgtggcggt tgtccgcggc
     3481 cagccggcgg atgcgaggca ggcgattgac caggcgatcg ccgcgggccg gccgccacag
     3541 gcgatcggcg cgacgaatgt gacgcgcgcc tggggcgtat tcgacaaact cggccagcgg
     3601 tggccggcac tggcgggggc gcgcgtcgtc gcgcccgcca gctattggtg gccgaacttc
     3661 ctcaagacgg ccaatctggt gaacgtggtc aaccaggtgt ccgacattgc gatcgtggcg
     3721 atcggcatga cgatggtcat catcgccggc gggatcgacc tgtcggtcgg cagcctgatc
     3781 gcgctggcgg cgatcatcat gaccaagctg atccgcgact actgcggcgg agtcgaggcc
     3841 ggcgcggcgg ccgttgtgct ctgtgccgcg gcggccatcg ccgcgtcggg cgcgatcggc
     3901 ggctgcaccg gtctgctcgt gacgcgggcc cgaattccgc cgttcatcgt cacgctgggc
     3961 gtcatgtcga tcgccagcgg ggcggcctcc atcgtttccc gcggcgagac gatcgaccag
     4021 gtgccgcggg cgatcggctg gctcggccgc ggcgctgacc tcggcgtgcc gaacacgacg
     4081 gtgctgatgc tgctgctcta cgtgattgcc gacgtggtga tgaagcggac gaccttcggc
     4141 cgttacttgt acgccgtcgg cggcaaccgc caggccgcgt ggctctccgg cgtgcccgtc
     4201 gatcgcgtcg tgacgctgtc gtacgtcatc tcggccgccc tggccgcact ggctggcgtg
     4261 atgcaggtct cgctgtttca cagcggcaag ccgaccttcg gcaagggcta cgagctgcaa
     4321 gtgatcgccg cggtggtcgt cggcggcacg tcgctcgccg gcggcgaagg caagatgttc
     4381 ggcacgctcc tgggagcgct catcatcgcc gtcgtgcaga acgggatgaa cctgctgggc
     4441 ctcggcggcg agtggcagcc ggtcgtgctc ggcgcggtga tcctggccgc ggccctgatc
     4501 gacaggctga agcggcggcg tgaagaatga cggcaaatct cgcgttctcg cagttgggca
     4561 ttttgctaga atccaaggca gcagcgcgac gtacgaataa gcagacagtg cgcgcacgct
     4621 aaccctagaa ggccccgatg gcccgcttcg tttcgttcct gatcctggtc ggcatcctga
     4681 ttttcctgac gatcatcttt taccaggtga tgtcgggatt cttcgtgccg ctgttcgtgg
     4741 cggcgctcct cggcgtcgtc gtgcagccgc tctatcgctg gatactggcc cagtgccgcg
     4801 gctatcgcta cgtcgcggcg ggcatcacga cgggtctggt ggcgttaatc gtgctgttgc
     4861 cgatcggcgt ggtaatcgcg acggcctcgc tggaagggct gtcgctggtc gatcagctgc
     4921 agctggccaa cgtccgcaca aagctcgacg aattgcggcg ggatttgggt ctgcaaattc
     4981 cgcgcgaaga ggacgtgcgg acgatcgaag ccaaactcac acggtggcgc aacctgcaat
     5041 tgaagggcga gtcgctggac atcaagtccg aagaagtcaa caacctgctc cagcgtgttg
     5101 acgaactgga gaagtggatc aaggagcagc agcagcagac cggctttgcc cgcgccgatg
     5161 tctcggcgct gaaggcgaac ctcgaggcgc tgcacaaagc cgagaccggc tcggtcgagg
     5221 ccgacaacgc cctgcaaaca gccgcagctg agtttcgcga gttcaagcgc aacttgctgg
     5281 gcggaacgta caaggcctgg ctcgccgagt gggccaatcc gaccgacgag caggtcgagc
     5341 agctccgccg cacgatcatg accaccgccg ggcccgtgct ctcgctgggc ggggacacga
     5401 tcgtcttgat cgtcaagctg atcgcgggcc tcttgatcat gatggtggcc ctgttcttca
     5461 tgctggccga gggcgcgcgg atgctggacg gcctggtccg cgtctcgccg ctcaacgagc
     5521 agcacgtccg cgagctggtc gccgagttcg accgcgcctg ccgggcgatc gtttcggcga
     5581 cgctcctctc ggcactggcc cagggcgttc tggccggcgt cggtttctac gtctgcggcc
     5641 tgcgcggctc ggtggccctg ctgatgctgc tcacgatggt cctggccctg gtgccgttca
     5701 cgggagcagc cgcggtctgg attcccgtct cgctgtacct ctacttctat cagggtgcga
     5761 cctgggcggc cgtcggactg gcgatttatg gcgcgatcat catttccggc gcggacaacg
     5821 tcatcaagcc gctggtgctg cacgggcagt cgaacctgca tccgctcttg gccttgctca
     5881 gcgtcatcgg cggcatcaag gccctgggcc cggtcggcat tctcgtcggc ccgatggtcg
     5941 tcgtcttcct gcaagtatta atgcgcctcg tgcatcggga gctgagccag atggaccgct
     6001 cgaattggat gttctggcgc ggctttggag gcgcggagcc cccgccggca gccgcggccg
     6061 ccgctgcggc gccggccgct gctgccgcga gcaccggcgc tgcgccggcc gagcccgcga
     6121 cgaccaaaac cgttccgccc gctccgccgc ccgctgctag cacgccgccc gccgatggca
     6181 acgggcacca ccagcccggc cagccgggaa agcaacacca gcaccacggc aaaaaacgcc
     6241 gaaggtagcg gcggctggcg cacccgccgc cgtttttcct ctttgcccag tgtcccgctc
     6301 gccgctatgc taccgcggct gccgccgggt ccacggaggt gcggccgcat ccaaggatgg
     6361 gagtacgagc gatgaacgca gtcgcgctga tggtggcgct cgccgcggcg ggcgtcgatt
     6421 accaggtcga aacgacctcc gacaagcagc agcaatacac gatccagatc gagcccgagc
     6481 tgctcttgcg cgtcgccggc ggcgaggaaa tccacagcga agtgccggcc gacgccggac
     6541 cgcttcagcg gctgtgcatc cgcatcggca tgacgaagcc cgtgcacgcg cccaccagcg
     6601 aggcggcctt ccgccagctg ctgctctcgg cgtcgcggtg ggcgatggcc gaccgcacgc
     6661 tgaccgcggc cgattcgccg cccacgatcc tctggcccgc gcggagcacc cccgagcaga
     6721 gccgcaacct ccgctacggc tggcagcccg acgccgaagg caagcagtcg tattacgtgc
     6781 agatcgacct ggcgatgctg cggtcgctgg ccgccggcga cgagatctat gcgccgatcg
     6841 acccgtccgc cggaagactg gaccggttca tcgtgaccat gacgcagaag gactatccgc
     6901 cgcggatcgc cggcgctgcc caggcgcccg cattcgtgcc gacagggccc ccgtttcaga
     6961 cgggcgggca gacttcgatc aacttactgc cgaccgacaa tccgcgctcg cagtttcaga
     7021 cgccgcccgc caccggcggc ggctttaccc cgaacaacac ggcgctaacg cccgtcgaga
     7081 ttccgccagc gcccggcggc cccgtcgcgc cgcgcgatta ttcgtggcca atgccgcggc
     7141 ccaatccgca ggcgaccatg ccgctggcga gcgtaccgcc ggcaaccgtg cagccgcagc
     7201 cagcgccggt cgtggacaat cggcccggcg cgttttcgcc gccggccacg aacacatatt
     7261 cgacggcgcc gcccaacaac tatcccgcga acaacatgct ggagccgccg cgctcgaatt
     7321 tcggcgggac ggcctcgcag caggtcgccg attcgcgcgc gtatcctggc gttacgccca
     7381 atgggacgta tgggacgaat gcggcttatg gtcagcagca aggatttggt cagccgcaag
     7441 gattgtcggc ggcggacaat cgaatcgcga tgcaaccgcc gccagtggcg gcgacgctgc
     7501 cgaccgtgaa tccgacgggc tcgctgactt ctggggcggt tccgccggcg ctgccggcga
     7561 aggagggctg ggggatgttt gtcgtcgtgc tgtttgcctt gttcttctcg atcggcggca
     7621 acttgtacct ggcctggacg gcgctggaat tccacaaccg ctaccgcaac gctatcgacc
     7681 ggctgcgctc ggcggcccgc agctcgtagc gcggaccttt cagccgccga cgctggcagc
     7741 gtcggctacg agcgcagcat ccggtcggcg agggccgtgg tggcttcgtc gtcgttgtcc
     7801 cacaaggcgg ccagcgactg cttgatgcgg cggcgggcgg tgcggagata gtggcggccg
     7861 gtctgcaggt ccagccgcag gccgccatcg tggtcgtggc tgttgcggag cagaaaatcg
     7921 agacgattca gcacgcagct gctggtatag acctccgtcg ccgcgtcggc cacgcgaccc
     7981 agctgatatt gccgatcgac gattcccagc tgataggtga cgagtagccg ctgcacgctg
     8041 ctgccgagca gattcacgag ccggcccagc tcggccgctt cgggctccag ctccgaattg
     8101 cggacgggga ttgtcggcga gacgagcagc gagccgagct ttcggccggc gaaccgcgac
     8161 aggcgcgtga cgttgcccag cggcgaatag atcgcgtcgc ggatgctctg cagctccagg
     8221 ccgacgtcgc gcatgccgac cagcgccgtg aaggcccgca gcacctcgtt ggccccttcg
     8281 ccaatcgtgt tgatcatcgc gtcgcggacc atccgctcca gcggctgatc ggtgaagtaa
     8341 gccaggccgc catgcagctg aaacgtatcg cggaggattc tggcaagcgt ctcggtcgca
     8401 aagaccttga gcatcgccgt ttccagcatg aagtcgcctt cgcccgagtc gatcagggcc
     8461 gcggtttgat aggtgcaggc ctccatcgcg aacacgccgg cctgcatgtg ggccagcttc
     8521 tccttgacca gctcgaagtt gccgagcgtc tggccgaact gcacgcggcg gttggcgtgt
     8581 tcgatggcgc gggccacgca gaacttcgcc gcgccggtgc agctcgctcc gaacgtggtg
     8641 cggccgaagt cgagcacggt cagcgcgacc ttcaggccct tgccgagctg gccgaggagg
     8701 ttctccttcg ggacgaacat gtcgttgaac gccaggcggc ccgtggccgt gccgcggacg
     8761 cccatttttt ccatccgctt ctcgacgacc tgaaagccgg gcatgtcagg cgtgacgatg
     8821 aacgccgtga tcttcgacgc ttcgtttccg gacactgtcg tgtggctccc ttcgacgggc
     8881 gtgcgggcca tcaccgtcag cacctgagcg acgccgccgt tggtgatcca gcgcttttgg
     8941 ccgttgattg tgtagccgcg gccgtcggcg gtcggcatcg cggtcgattg cacgttggcg
     9001 gcgtcgctgc ccgcttcggg ttcggtcagg gcgaacgcgc tgatccactc gccgctggcc
     9061 agcttcggca gataggcttt ctgctgggcc tcggtgccga acagcaccag cgcccgcggg
     9121 ccgatcgagt gatgcgcgtt gacgaacagg gccgtgctgc cgcagtggcc gccgagcact
     9181 tcgagaatcc gacagtaggc cgtttgcgac aggcccaggc cgccgcagtc cttcggcagg
     9241 cacgcgccga ggacgcccaa ccggcccagg ccttcgatca ccgggcgcgg gatttccgcc
     9301 tggcggtcga tggccacggg atcgatcttt tcggtgcaga agctccgcaa ccgttcaacc
     9361 aggtcggtcg tttccggatc gcgcgcgtgg tcaggataag gcagcagccg gccctccagg
     9421 tactggccga agaacagtcc cttgacgaag ccgacttgcg acaggcggtc gccgagaatc
     9481 tcctcggcct gggcgatttg ctgctggcga tcgggtgcgg ccatgagtct cgctcgatcg
     9541 gaggcggggt cagggaattt tacggcggca gcgtcctcgg agcgacccgt cgcaaacgcc
     9601 ctatagccct caaaaacgaa cctgcccacg ggggatggtg cggtgtataa tccatcaaag
     9661 ttcttgaaaa acctacgccg ctaccggccg caatagatgc ccaggaaaga gaacatcgac
     9721 cggattttgc gggactggcc ctacgatccc cacggcatca gcgtgcggaa aaagaagggg
     9781 gacgacggcc gcgaagtcct gcaaatgcgg ctggacctgg gcatgctgca gctcgagacg
     9841 gccggccgcc cggacggcca gcggccgcag ggagcggatt cgtactacga ctatctgatc
     9901 ggccgtacgc tcgccaacgg cgacgaatac gtcatgaccg aggacgactg cgaggaggcc
     9961 gatcgcgagt tcgtgcagta ctaccatcgc cggatttgct ggctggccct gcgcgaattc
    10021 cagcgggcgg tgctcgacgc cgaccacacg ctgggcatga tggatttctg caagaagcac
    10081 tcgcccgacg agcagtggac cctctcgcac gagcagtacc gcccgttcgt cctgttccac
    10141 cggacgcagg ccgcgtcgct cgccgccctc agcgacaaag gggccgaggc cgccgtggag
    10201 gagatcacca agggcctcga tcagatgcgg caagttttcg tcgactacga agccgaggag
    10261 gaattcgacg aggacgaact ggtccagcgg ctaatcgagc tccgcgagtc gctgcggcag
    10321 cacttccgcg tcggccgcac gctccaggag cagctggccg acgccgtcgc cgccgagaaa
    10381 tacgagctgg ccgccgaaat ccgcgacgag ctcaaccgcc gcgattcaag ggcgcggtag
    10441 cgtccggcgg cgagccgatg gtgaggaggc tgccgcgttt tggctcggcc gagttgctgc
    10501 tgcacgaacc gcaaccgctg ccgcagcccg ctttcgaccg gccgaagaat gctcgatagg
    10561 cccgcgcagc gacaacgatc gccgcgacgg cgaacaggcc cagcgcgaga taatcggtcc
    10621 agtgcatgag tcccccgtcc cgcaggccga aactcttcgc tctcaagtat cgtcgttcat
    10681 cctacggaaa gccagatcgc cggctgacgg gagatcgcga tttgtcgcct cctgctggct
    10741 gcgatatgtt gaggtagctt cacgcgctaa cactcgcgga gtgaatcgat ggccactgcc
    10801 ttcctgtttt ttctggtaag cgccccggtc gcttgggggc aggcggccgc cgcgcctccg
    10861 accgcggaag aaatcgccgc ggccatcggg cggctggggg acgacagttt cgatgtccgg
    10921 cagaaggcga ccgagtggct gtggcaggcg gggcccgccg cggagacgca gctaagagcg
    10981 gccctcaaga gcaccgaccc ggaggtccgc acgcgggccg cgtcggtgct cgacaaaatc
    11041 cgctttggcc tgcggcctga tacgcccccg gatgtggcga ttctcatcga ccagttccgc
    11101 cacgcggggt ctgccgcgct gcggaggcag gcgctcacgg cgctgcagga caagggtcag
    11161 tgggccgtga tcctctcgct ggtccgccgc gagaccgatc ccaatgaacg gcgcaacctg
    11221 gcgacggcga tctccgccga cgccggcaag ctcctccgcc cgctcttggc caagggagac
    11281 ttcgaccagg ccgaacaggt gctggagctg accgccgtca acgaagccgg cctgacccag
    11341 ctcgcgacct ttctgctgct cacccagcgg ctggagtcgc agatcgaagc agtgcggcag
    11401 cgtttggcgg cggacccgca ggaggatgag tggcgacggc tggcgctcta cctgcgggcc
    11461 aaaggcgact ttactgccgc ggcggatgcg gctcagaagg cgggcgacgc tctcttcgca
    11521 gcgaaggtgc tggccgagag cgggcggttc gccgaagcgg cagttgcggc ccaaagcgct
    11581 ccgaaaggga gcgggaatcg ttccgacgca gacgcattcg ccgccgcgta ttttgacctg
    11641 gcccgcgacg agacgcagtt ccagcggacc atcgaactac tcaagcaggc cgccaacatc
    11701 gacaaaatcg ccgacaagcc gcagccgatc gggccggccg atccctttgg ccagccggcg
    11761 agcagtccca cggccgccag cgcgtggcgg ctggccgagg cgctgctcat cgccgagcgg
    11821 gtcgaggaag ggttggcggt gctcaagaag acgcatcccg ttcaggccca cgccctgctg
    11881 atgaggcagc accgccatca tgacgccctg gagttcgtgg gcgtgaccgc cgagaagaag
    11941 ctcgaccgcg cttggctcga ttcgctgccg ggcggcctgg gcgacgtgaa tcagcagctg
    12001 tcggttcgct tcggcctcgc ggcgcaggtt gcccggcagc tgcgcgagct cggccggatc
    12061 gagcagacca agcaaatcgc ggaaacgctg cgggccattg ccgccgccga aggactaaat
    12121 ggcttccgct ggtccgcgct ggccactttg cattggcagc tcgatcagta cgacgaagcc
    12181 ttcgccgccc tggagcaggc gctcgcctcg ggccagcagc cggcgagtgc gttcaccggg
    12241 ctcgcccgga cgcaggcatc gctcgccgcc gcctggttca ccgagattac gacgcgcgat
    12301 ccattggctg accgcaaagc agccatcgct caggccattt ggctggtaac tgcccagccg
    12361 ccggccggca agctgcccgc caactggcgc gacctggtcg agcggtcggc cgcggaagcc
    12421 cgcaagctgc cacagggaca gcgcggcgcg aacctgatgc tgccggccga catcatccga
    12481 gtgcgcggcg acttgccgct ggcccgcaaa ctgtatgagg aaggcgagga ggatttcgcc
    12541 gccgccacgc gtcaggccgc cgatgtggcc gccgccttgg aggactggcc cgccgctgcg
    12601 gccaaatacg gcaagctggc cgggccggac aacagcgatc tgttggtagt gtatttgcac
    12661 ggccacgcac tgacgaagac cggtcaggaa aaggaaggca aagagtcgct gcgcctggcc
    12721 tcgctgacgg ccctggcgcc cgacgttcgc tacgcgatcg ctctggggtt acaggagcgc
    12781 ggactgaagg agttggccgc cgagcaattc gagatcgtgc ggcggacggc actgcccgat
    12841 tcgtcgatct cggtaaatgc cgcgcagcgg gtcggcaacc tggtgaacga ggagcagccg
    12901 cagcgggcct gtgattgctg gcggcagctg cacctgcaca tcctgaacag caacagcaat
    12961 tttctggagg ccgagggcta tctctcgctg ccgcagatca tgcacaaggt gcgggcccgg
    13021 gcggcgctcg ccggcggcaa ggcggaagag gtcgcggcag aactggagcg ctgcgagaag
    13081 ctgctgccgg gcgacgtgcg gctgacggtg gatctgacgc cgaagctcga cggggccggc
    13141 ctgcgggcca tcgccgacca ggtcttcgag cggtcgatcg cctcccaccg gcgtgtgctg
    13201 gaagagtttc caggcagccc gacgtttctc aacaacgcgg cctggatttg cgcccgcgcg
    13261 cagcggcagc tcgacgaagg actcgcattg gccgagcggg ccgtgaaggc cgcgcccgac
    13321 gaaggttcgt accacgacac gctcgccgag gtccacttcc agcgcggcga ccgcgacgcc
    13381 gccgtcgctg ccgcccgcaa ggccgcagaa ctttcgcccg agaacaagct ctttgccaaa
    13441 cgcctgaagc attttcagga agatcccgtg aagacgctgg attccgtcaa cgagtgagat
    13501 gcgtagggtg tgacgaggcc gccgcggccg acggcacacc ggagccgaag agaacgggtg
    13561 atggtgggcc ggcgctcgcc ccgctcgctg gtcccaccct acgggcccag ccgccaccag
    13621 aggaacagga cgcaggacca gtagtacgcc gcaaacgagg caaagagcgc agaggcctcc
    13681 agcagccatt tgcgccgctc ggctcctgct aaagccgcgg ccaacacgct gaagggcaat
    13741 gcgccgagga agccggccag gaatccccac tcgatcgcca gccccaggcg gcggcagaga
    13801 tgaaaataga gtccgcccag gccgaggaac gccagcccaa ccgcgtacga cgagccgcgc
    13861 cgccagctca cgagcggcga gttgcgaact gaccagctgc ggctgatcgt cagctgaaat
    13921 gcggcccaca gcgccagcgg ccaggtgaag acatatagag gctgcacccg gttcagtacc
    13981 gcgctgccgg ccaatccgca caacaaaatg gctgtccaaa acgcagcgtc gatccacgct
    14041 cgcttctttg gcgcgagcca gaggcagaca ccgctcgcga gcaggcagca aagcagtgcg
    14101 gccgaacacc aggcgtacca gcggccgaaa tcggtcgttt gcggcagcgt cgcgggataa
    14161 tcgcgttcgc cgcccaacag cacgccggga ttgaagcgcg cgccgcccgc cgcatcgaga
    14221 accgacagcg gcgaggacat cgccagaggc tcgcgcccca agtagaggca gaccgcgcgg
    14281 gacggagtgc gcaggtagag ccggccattg gccaatgccg gcgcagtcca gcagacttcg
    14341 cccggaaaga cggcggcgcg gcccagctct tcgtatcgct cgctcgaggc ccgcgcgaga
    14401 atcaactcgc cccggtcgtt gaacaagatc aacttgccgt cggcggcgac gatctgggcg
    14461 tggcccggtt ccgccgacga ccagcgaatt tgccccgtga gaaagtcgag cgcgcgaaat
    14521 tcgccccgcg acggccggtt cagccgcgac tgggcctcgc ggaggtcgaa gccaaaaatc
    14581 gtttcgccgg cgagcacgct cgaggccacg tcgttcgaca atttctcgtt gaaccagact
    14641 tcctgcggct ggcagcgctc cccatcgggc ttgagctcca gcagcgtcgc gccgctgtga
    14701 aagggcccgg tcttcagcag gtagggctcg cggtagatga gcgcggttgc gtgctcgtcg
    14761 tagccctgcg acagcggcaa ctcccaccac aattcgcccg tgcggcggtg aatgcaggcc
    14821 agcgaattct gcaggagcgc gacgacgagc ggctggccgc gccactcgat gggcacgggc
    14881 gtggaatagc tggcaggatt cgagcccgct ttccagacgg tcgcgccatc cgcggtgctc
    14941 agggccacga cgctggcccg ctcgccgccg accggcagga tcacgagtcc gtccaccacc
    15001 agcggcgagc aggaataacc gaagtcggtg ccgcggccgc cgaagagctc gttgatgttc
    15061 accgaccaga ccagtcggcc tgcggtggtg aggcacccga cgaggccctg cggcgacgcg
    15121 aaatagatct gcccgccgct gacggtcggc gtggatcgcg gtccgggata gagcccgccg
    15181 ccgtcgtagg gccagccgta ggaatacgac cagatcgtcc gccccgtctc ggcgtcgaga
    15241 caaagcaccg attgctcgta gagcgactgc gactgcgtat agacgcggcc gccgctgacg
    15301 gcaaagcccg aatagccctg gccgatttcg cgggcccaca ggaccggcgg acctgcgggc
    15361 gggaaggaat cggccagccg ctcgccgggg gcgtggccgt cgtgcgccgc cccgcgccac
    15421 tgcggccagt cgccgtcctg agcacgaccc gccttcgccg ccagggcggc caggccgatg
    15481 gccgcagcga ttgcgaggga tatgccgcgc tgaactgtgg gcgggccgtg catcccgtaa
    15541 cctatgcttg gatggtttct atcctaccga cccgccgtcg cgaacgaaca ttcgcgcgcg
    15601 aatttcaccg tgggagcagc gcatgtcgtc cttctcgctt gaggacaagt tcccggacct
    15661 cgtgccgatt caaaccgccc cgtccctgca cacggtgaac gggatcggga ccggcattta
    15721 tggccgccgc gactacgacg aggagacggg aacgtacgtc acgacctatt gctggtgcgt
    15781 gctcttcatt ccgctgtttg ccgtggcggc ctatcgcgtg gccgacgcgc ccggcggcgg
    15841 ctactacttc ctggggaaga cgcggctgtc gggtttggcc aaggcttgga acgcgctcct
    15901 ggccgcgtgc atgattgccg gacttggcgg cgggaccttt tgtgcgtggc attactcgcc
    15961 cgagaatgtc gcccttcgcc agttggccgc ggccgaacag agcatcgccg ccggcgagca
    16021 tgccggcgct gccaaggagt tcgagaaact cgcgctgggc cgcacgaagc aggccgcgcc
    16081 ggcccgcgag cggctggaga agtggctcgc cgcgccgccc gcgggcgctg aagccagcga
    16141 ctattttgaa gtcttcaagt cggccgccgc actgaaccag atggggcgct ggccggccgg
    16201 cccgcaggcg ctgcacgacc gggcgctgac gctggccgag ctcgtccccg ccgggcagag
    16261 cccgctggcc gtgtcgctgc tcaaggcggc cgaacccctc gacgagaatc ccgcggcctc
    16321 ggcggcgcgg aaacgagcac tgctcgaaaa gctgcaggcc cgcgcgccgc aggacctgga
    16381 gctgaccgtg ctcttggcgc aggcgctcga gcaggaaggc gatttcgagc ggtgcgagaa
    16441 gctgcttttg ccgctggcgg acaagctggc cgacaccgag ggcgcgcgga tcctcggcca
    16501 gcggcacctc agccagggca agttcaatga agctcacgca ctgctagcgc cgtactgcaa
    16561 atcgcggatg gcccagtatc gcgccgcgga ggaagccttc gaggcggcct ataagcaggc
    16621 ccaggagacg gagatcaacg tcctggaaag gaacgaggcc ttccgccgcc gctacgacgc
    16681 ggccagcgac gagcagcgtc agcagatggt cgcggaaaag atcgatgagg cagtgaagaa
    16741 cgacccgcaa gtcgccaagc agcgcgacgc gctgcgccag gcgtcgcggg tcgtgcccgt
    16801 cgccctcgat ttcggcgtgg tgcagcttta ccgggcacag ggcctgagcg atccggccga
    16861 gcggaaagcc gagttgcagc aggccgagac aaccttcctc tcgctcggca acgcgatcgg
    16921 cgacaccgac gagtacaagc tcaacctggg caaggtttac tactggctgg gaaagtcgaa
    16981 ggagggcagg aagctgctcg acgaaatcct gtttcgcgcg gcgaacaatt tcgagttgcg
    17041 gatggcggtg gcccagacgc tccgcgaagt cggcgcggag acggattccc ggaacctcgt
    17101 cgagcacatc tataacaacg ccacgtcgga cgaccacaag tacgaagcgg ccgagctgcg
    17161 agccttgctg tttttcgatc tcgacgacga gatcgcgtgg ctgaaccgcg cccggccggg
    17221 cgaccccgac gtgaaggcgt cgctggccct ggcccgcggc cagaaggggc tgcaggacgg
    17281 cgacgagaag gccgccgagc agaacctgcg gcaggcggcc gagctgtaca cctcgtttcc
    17341 cgagtcgatg tcgtcgctca acgacgcggc cctctgctac agcggcctgt tccactcccg
    17401 cggcgacacc accgacttgc ggcaggctgc ccggctgctc gatcgggccg tctcgctcaa
    17461 gcccgacaac agcgtgctga tgatcaacgc cgccgacctc tacttcgacg tggccctggc
    17521 cgacgccttc gcgacgcaga tcgacctgcg gctgatccac tcggttggcc gcggcgggct
    17581 gctgggattc ttctaccggg acggcgcggg ccgcgacgaa gtgatcgctc ggctgaagac
    17641 ctcgcccgcc ctggccaagg cgacgcagta cttcgacaag tcgatcctgc tggcccccaa
    17701 gcgcggctcg aattacgacc aggcctccgc cctctacacc ctgctcgacg acaatgcggc
    17761 cctggagcga atcctgcggc agatcgacga cgcgcatccc gacacgcagg aaaccgtcac
    17821 cgaaacgctc aagtacctgg ccggcgatta cgacaagaag acagcgcaga cggtcgccac
    17881 ggcccaggcc gccagccgca agctggtgga ccattggcga gccgcgccgg ccggcgacaa
    17941 gcggaaccgg tcgctggccg ccgcgcagat ggcggcctgg ggggcgctga tggccaggtc
    18001 cgcgtcgccc gccgactcgc ctccggatgc ggaggagctg ctgaagctgg cggaggaggc
    18061 ggtccgcacg cacgagtgct cgatgaccga gcagacgctg atctcttcgc tgctctatcg
    18121 cgtccacgcg cagaacgtcg cgaagtcgcc cgcctaccgg caattcgccc agaaataccg
    18181 ccgccacctc acgccttacg agacgctgct cctcgcgctc gaccagaagc cgctggcccc
    18241 ggcgatcctg gcccacgccg atttcaagcg ggccgtggag ctatcgaagc agcggctagc
    18301 gcggtttccc cgcgcggtag ccgccgacga ctggatgatg ctgcgctacg ccgaccccgc
    18361 ggcggccgag cagatcgccc ggcggctggg cgacggcacg ctcgagggcc gcgagtcgca
    18421 aatcgatctc cgcctgcatc cggccagctc gcactttgct gtgttggaat cgtggcggtt
    18481 gcaatcgacc ggccgcgccg gcgaggcgac cgcggtgttc cagaaactga acaagcaagg
    18541 cgtccgctgg ccgcagccct agcgcttgtg gaatgccagc cgggcgatgc cggccgcacc
    18601 gatgtagccg gcgtcgccgc ccagctcggc aaagtcgatc gtcgtcttct gggcggggat
    18661 cgggaaggcg tgcgagcgga ccacgccgcg aacgtgctcc aaaaagcgcc gcccgaccgg
    18721 atcttcgtgg ccgccaaagt tcatcgcgcc gccgagaatc actgcgccgg gatcgatcgt
    18781 gtgcattagc gagacaatgc ccaggcccag ccaggtgccc agttccatga tgcactcgaa
    18841 cgacagcgaa tcgccgcggt cggcctcttc ggcgatcatc agggcggtca gcggcgtttg
    18901 cggcgtgatg cgggcctgca ggctcgtcgg ccggccaccg tcgatcaaat cctgcacccg
    18961 atcgactagc gccgtcgcgc tgcagtaagc ctccaggtgc ccgggctggc cacagccgca
    19021 catccgggcc agcggcgagc tgtcgatgat gatgtgtccg cactcgctgc cgtggctgtt
    19081 ttcgccgtcc accgacaggt cgccgatgat gatgccgccg ccgacgccgg tgccgagcgt
    19141 cagcatgatg atgctgtgat gtttttgccc gctgccgacc caaaactcgc cataggccgc
    19201 ggcgttggcg tcgttggcaa agacggtcgg caggccgaac gcctcggcca cgcagtcgcg
    19261 gatattgaaa tagtgccagt gcggcagatt gtgcggctgc aggaagatgc cccgcggtat
    19321 gtccatcgtc cccggcgccg ccaggccgac tcccgccagg tcgccgatat tcagcccgca
    19381 ggtcgccagc atttcgtcca ccgcgactct agcccgcttc accgcctccc gcggaccctg
    19441 ctcctcctcg gtcgcaatct tcgtattgca gaccggccgc cccgcgtcat cgaccacgcc
    19501 cagcttgata ttcgtcccgc caacatcgaa gcccaaaaac agtggccgct gggcactggc
    19561 aacctcaatc atcggggatt ccgtaggcat caggcgaata tataatgtgg gccactcgct
    19621 ccgcgagtgg ccgatgcggc ctcgatacgg atgctggtga ttggaccatc gtcctggcaa
    19681 cgcgcggagc gagttgccta ctatccccat gctcgacatc ctcgtgatcg cgccgcatcc
    19741 cgacgatgcc gaactgggcg cggcgggggc aattctgaag ttcaaggcgg aggggaaaaa
    19801 ggtcggcgtc ctcgatctga cgagcggcga gccaacgccg tttggttcgt tggaaaagcg
    19861 cgcggccgag acggctgcgg cgacgaagat cctggggctc gattggcgtc aaaacctcgg
    19921 cctgcccaat cgcagcctgg aggcgacgct ggatgcgcgg aagcagctgg cgggcgtcat
    19981 tcgccagact cggccgcggt ggctcttcgc cccctactgg gtcgatgccc atcccgacca
    20041 cgtcgcggcg acgcagctcg tcgagggagc ccggttctgg tccaagctga ccaagacgga
    20101 catgccgggc gagccctggc acccgcagcg gatctacaac tactactgcg tccacttaaa
    20161 gctcgccgct cagccggcct ttatcctcga catcagcccc ttctgggaac agaagctcgc
    20221 cgccattcgc tgctacgcca gccaattcat cgaaggccgc ccctccgcgg cgcccacctt
    20281 cctcgaccag ctccgcgacg aagccgccta ttttggcaaa tgcatcggcg tcccatacgg
    20341 cgaacccttc cactgccgcg agccgatcgg cctgtccagc atgcaggacc tggtctgacc
    20401 ttcccggcca tagtggattc cggatcatat aacgataact gtatagtaat ggccgatttt
    20461 ttctgccgct cgcgtcgccc cggcgatccc aactctctgg cagttcagca cttccgacgc
    20521 gatacgggtg gatcgctcac ataagagtta gaagcagtag aagcccgtcg cgcgagttat
    20581 cgttatattc cgcgcgaacg ccaggggcgg cgattccgcc tttgagcgca acgaccaaat
    20641 tgagaaacag cggacgggca cggcgaccgg tcagcaaaaa tggcagcccg cgcgccaatg
    20701 ggaacgatac tgcaaatacc atgccaatgt tcactatcgg ctgagcaacc ggcggcgagc
    20761 tcaaccggaa cctgggcccg ccgacaggcc atcgtccagc agcaggattt cgatcgtttc
    20821 gccggcggca aagacgcggt cgccggcggg gaagcaggcc agcgcgttgg cttcgctgag
    20881 cgttcgcaag tcgcccgagc ccttccacgc cagcggcgtc acctcgcagc cggtcgccgc
    20941 gatgtgtgac gcggagggcc agtaggcggg ccgctcgccg cgctgccgat gctcgcggga
    21001 cagcctgccg cgcattttcg taagaccgat cggcccgcgg ccgctcagga cgccgatcgc
    21061 cggacggacg aacagctcga agctgaccag cgtgctgacg gggttgccgg gcaggccgaa
    21121 gaccagcgtg gacactccgg cgctgcgcgg gtggaccccg aaccacagcg gtttccccgg
    21181 cttcaggttg actttgtgaa acacttgtcg cacgcccaac tcggccagga cctgcggcac
    21241 aagatcgagc acgccggccg aaacgccgcc ggagaggacg agcacgtcgt gagccagtcc
    21301 cgcggaaacc ttggaacgca gatcggctgc atcgtcgcgg gcgatgccca ggtcgatcgg
    21361 cgtcgcgccg gtttgccgga cgagagccgc caagagcggg ccgttgctgt tgcgaatctg
    21421 cccgggaccg ggcatttcac cgggctcgac cagttcgttg ccggtggcca gcaccgcgac
    21481 gttaggccgc gggacggcca ggacgc
//
LOCUS       DASRJB010000387         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1234616, whole genome shotgun sequence.
ACCESSION   DASRJB010000387 DASRJB010000000
VERSION     DASRJB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234616"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFB80_10215"
     CDS             <1..246
                     /locus_tag="VFB80_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34185.1"
                     /translation="ALPRLRDVHQKDFLIDYTQQGKRAYYIASRIESDRDEDAPADVL
                     ARLSLHFEMCAYGLGEIRREWERRDGDDETPRPLIIN"
     gene            complement(253..1674)
                     /locus_tag="VFB80_10220"
     CDS             complement(253..1674)
                     /locus_tag="VFB80_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34186.1"
                     /translation="MTRRQHQSCGRFTWPATRRQFLARAGAGLGLLALSDLLEPATAA
                     DRQPPSAGLMAPRPPHFAPKAKSVIWLFMEGAPSAVDLFDPKPELDKSDGKKIEINVF
                     NGNPGPLMKSPFKFQRHGECGAWVCDQYPHVAKHMDEIALVKSCFTESNDHVPALYQM
                     NTGVARAGLPTAGAWVTYGLGSENGNLPGFVVLGNNQGIKGGPLNWSAGFLPTGYQGT
                     LFRSEGSPILNLQRPGGVGREDQRAQLDLLARLNHEHLQSHAGEPDLTARIESFELAY
                     RMQLEAADLANFAQETAQTQEFYGLNDKTTRSFGGKCLLARRLVERGVRFVQVYSDGE
                     WDAHADLKGNHTGHCAATDRPIHALLTDLRRRGLLDSTLVIWGGEFGRMPVSQGAGGR
                     DHNPKGFLLWMTGAGIRGGASFGETDEIGYKAAVDPVTVHDLHATMLHLLGIDHKRLT
                     YLHNGRRYRLTDVAGNVLTKILA"
     gene            complement(1687..>3132)
                     /locus_tag="VFB80_10225"
     CDS             complement(1687..>3132)
                     /locus_tag="VFB80_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34187.1"
                     /translation="EEVARRRERETRWQERRGRAESQLSSWLEETRKPHAAALRTAKI
                     GSLPVGDDEKKLLQEQPDSEAAKKLAKKHEKALAISDEDFRRVFSEEQRQKWDALKKE
                     VADIQRESPPALPTALAITDTRPEPAATFLLQRGDFYARQEPLELGFLTVLTTEKSPA
                     EYFAAARRDGPPGRSTLQRRALADWITDEKHGAGALLARVMVNRIWQHHFGEGLARTV
                     SDLGVRSERPTHPELIEWLAHDFAAGDWRLKRLHRLILTSAVYQQDTSFDERRSAVDP
                     DNRLLWRRRPQRLEAEILRDAVLSVSGTLNLRPYGPAFKPPIPAEAIVARNTKDPYPQ
                     DAKDMSDTRRRTVYMFHKRVVQNPLMQVFDGPDAAVSCGRRTTTTVAPQALALLNDRL
                     LRERAADFARRLLAECSEEPAQQVERAFWLALSRAPSDSERAASVTFLESQRRQRAER
                     DTSASTDDVRLQSLADFCQALFGANEFVYVD"
BASE COUNT          492 a         1054 c         1001 g          585 t
ORIGIN      
        1 gcgctgccgc ggctccgtga cgttcaccag aaggactttc tgatcgatta cacgcagcag
       61 gggaagcggg cctactatat cgccagccgc atcgaaagcg atcgcgacga ggacgcgcca
      121 gccgacgtgt tggcacggct aagcctgcac ttcgagatgt gcgcctacgg tctcggcgag
      181 atccgccgcg aatgggagcg gcgggacggc gacgacgaaa cgccgcggcc gctgatcatc
      241 aactgacgcg cttcatgcca gtatcttcgt cagcacgttc ccggccacgt cggtcagccg
      301 gtagcggcgg ccgttgtgca gataggtcag ccgcttgtga tcgatgccca ataggtgcag
      361 cattgtggcg tgcaggtcgt ggacggtgac cggatcgact gccgccttgt agccgatttc
      421 gtcggtctcg ccgaagctag cgcccccgcg gattccggcc ccggtcatcc acaggagaaa
      481 gcccttgggg ttgtggtcgc ggccgccggc cccttgcgac acgggcatgc ggccgaattc
      541 gcccccccag atcacgagcg tcgagtccaa cagcccgcgg cgccgcaagt cggtgaggag
      601 cgcgtggatc ggccggtcgg tggcggcgca gtggccggtg tgattccctt tcagatcggc
      661 atgcgcgtcc cattcgccgt cgctgtagac ctgcacgaag cgaacgccgc gctccacgag
      721 ccgccgcgcc agcaggcatt tgccaccgaa ggagcgagtc gtcttgtcgt tgagtccgta
      781 gaactcctgg gtctgagccg tttcctgcgc aaaattggcc aggtccgcgg cctccaattg
      841 catccggtag gccaattcaa agctttcgat cctggcggtc agatccggtt cgccggcatg
      901 tgactgaaga tgctcgtgat tgagcctggc caacaggtcg agctgcgctc gctggtcttc
      961 acgccccacg ccgccgggcc gctggaggtt caaaatcggg ctcccttcgg agcgaaacag
     1021 cgtcccctgg tagccggtcg gcaggaagcc ggcactccag ttgagcggcc ctcccttgat
     1081 cccctggttg ttccccagca caacaaagcc cggcaggttc ccgttctcgc tccccagccc
     1141 gtaggtgacc caggcgccgg ccgtcggcag tccggcccgt gccacgcctg tattcatctg
     1201 gtacaaggcg ggcacatggt cgttcgactc agtgaagcac gatttcacca gtgcaatctc
     1261 gtccatgtgc tttgcgacgt gcggatactg gtcgcagacc caggccccgc actcgccgtg
     1321 ccgctggaac ttgaaggggg acttcatcag cgggcccgga ttgccgttga aaacattgat
     1381 ctcgatcttc ttgccgtcgc tcttgtccag ctcgggcttg gggtcgaaca aatcgacggc
     1441 actcggggcc ccttccatga agagccagat cacgctcttg gccttgggtg cgaagtgcgg
     1501 cggccgcggg gccatcagac cggcggacgg cggctgtcga tctgcggccg tcgccggctc
     1561 tagcaggtcg ctcagcgcga gcagccccag tcccgcgccg gcccgcgcca ggaattgccg
     1621 ccgggtggcc ggccatgtga atcgtccgca ggattggtgt tggcgacgtg tcatggcttt
     1681 ggcctctcaa tccacgtaca caaactcgtt ggcgccaaat agcgcctggc aaaaatcggc
     1741 gagcgattgc agtcgcacgt cgtccgtcga cgccgaggtg tcgcgctcgg cccgctgccg
     1801 acgctgactt tccaggaatg taaccgacgc ggctcgttcg ctgtcgctag gcgcacgcga
     1861 caaggcgagc cagaatgccc gctcgacctg ctgggccggc tcctcgctgc attcggccaa
     1921 gagccgccgg gcgaaatccg ccgcccgctc gcggagcagc cggtcgttga gcagcgccag
     1981 cgcctgcggc gcgacggtcg tcgtggtgcg gcggccgcaa ctcaccgctg cgtccggccc
     2041 gtcgaaaacc tgcatcagcg ggttctgcac cactcgcttg tgaaacatgt agaccgtgcg
     2101 gcggcgcgta tcggacatgt ccttggcgtc ctgtggatag gggtccttgg tgttccgtgc
     2161 cacgatcgcc tcggcgggaa tcggcggctt gaaggccggg ccgtagggcc tcaagttcag
     2221 cgtgccgctg accgacagca cggcgtcgcg gaggatttcc gcctccagcc gctgcggtcg
     2281 ccgccgccag agaagccggt tgtcgggatc gaccgcggac cgccgttcgt cgaacgaggt
     2341 gtcctgctgg tataccgcgc tggtgagaat caaacggtgc agccgcttca gccgccaatc
     2401 gccggcggcg aagtcgtgcg ccagccactc gatcaactcc ggatgcgtgg gccgctcgct
     2461 gcgcacgccc aaatcgctga ccgtccgcgc cagcccttcg ccgaagtgat gctgccagat
     2521 acggttgacc atcaccctgg ccagcagcgc gcccgccccg tgcttctcgt cggtgatcca
     2581 gtcggccagc gcccgccgct gcagcgtgct gcggcctggc gggccatcgc ggcgggccgc
     2641 ggcaaaatac tcggcgggtg atttttcggt agtcaacacg gtcaggaatc ccagttccag
     2701 cggctcctgc ctggcataga agtcgccccg ctgtagcaga aacgtagccg ccggctccgg
     2761 cctggtatcg gtgatcgcca gcgccgttgg cagggccggc ggcgattcgc gctggatgtc
     2821 agctacctct ttcttcagcg catcccactt ctgccgctgc tcttcgctga acacgcgacg
     2881 aaaatcctca tcggatattg ccagggcctt ttcgtgcttc ttagcgagtt ttttggcagc
     2941 ttccgaatcc ggctgctcct ggagtaactt cttctcgtcg tctcccacag ggagcgagcc
     3001 gatcttcgcc gtgcgcagtg ccgcggcgtg cggctttcta gtctcttcca accaggagct
     3061 aagttgcgac tcggcgcgac cgcggcgctc ttgccaccgg gtttctcgct cgcgccgccg
     3121 ggccacttcc tc
//
LOCUS       DASRJB010000388         7253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_671287, whole genome shotgun sequence.
ACCESSION   DASRJB010000388 DASRJB010000000
VERSION     DASRJB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671287"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..298)
                     /gene="rpmA"
                     /locus_tag="VFB80_10230"
     CDS             complement(47..298)
                     /gene="rpmA"
                     /locus_tag="VFB80_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZN34188.1"
                     /translation="MAHKKGQGSTRNGRDSNPQRRGVKRFGGERVVAGNIIVRQCGTK
                     FHAGRNIGMGKDYTLFALVDGTVRFDQEGRRVNIDPAAN"
     gene            complement(723..2540)
                     /locus_tag="VFB80_10235"
     CDS             complement(723..2540)
                     /locus_tag="VFB80_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN34189.1"
                     /translation="MSLLASPLLFADFFDRFRIQLADPIVNWLIPVWILCIGAAAGLA
                     LTAAIWGVLKLLSLLPGIGTLADAPGPRRLATAVLTILLLGLAVGLYLTNVPNAQAAP
                     LLDRIWALAGAALACWVLATALVTLVSRRALAETRIAIREGVLWPLFITALVMTGFAV
                     FGLFIVRKPTELLESLVRGPQLALEGDATMTRDLAAPANPVEDPVEQEVPVNFRRDEI
                     RGLVFKSDQRVKLRSEPTGSTTDPSLVLTLDIPAGESKTWRREQTGTTPFREPVITKL
                     YAKNQGLAKATLQITPIREVATPEMVLVPIMAVGMAAIFFLYLLQRAALPKLAAVALS
                     TAKSDIAQPLYAILMAIGVFLLLLFVFIPYNTFGDDIKQLKDSGLSLILVLCVFQSVW
                     AASSSVADEVEGKTALTVLSKPVSRRDFILGKFTGIAWSVGLMCAIFGLLLLVTVAYK
                     PIYDFREGGYEINHQQQNQDPTWHDCHREMVQIVPGIVLVFLETLVIAALSVAISTRL
                     PALANFIISFSIYVLGHLTPLLVQSQVVANQLPPVIFFGNLIATVLPVLDHFNIQASV
                     AAGVPVPIVYLMWSLVYCILYSSVAMLLALTLFEDRDLA"
     gene            complement(2660..2944)
                     /locus_tag="VFB80_10240"
     CDS             complement(2660..2944)
                     /locus_tag="VFB80_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZN34190.1"
                     /translation="MNGTEIVRRRVLYAGNVQGVGFRYTTQHIAQSYAVTGFVRNLSD
                     GRVELVAEGEPPVVEAFVAKVASTMARYIRGAESADLPPTGEWKSFTIAR"
     gene            3063..3257
                     /locus_tag="VFB80_10245"
     CDS             3063..3257
                     /locus_tag="VFB80_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34191.1"
                     /translation="MDQADSVEIYCPNCRQHIVPLKASVSWYCPICAWQFSDAEIDRQ
                     GGGPACTKPPESKKDRPRES"
     gene            3522..4379
                     /gene="lpxA"
                     /locus_tag="VFB80_10250"
     CDS             3522..4379
                     /gene="lpxA"
                     /locus_tag="VFB80_10250"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZN34192.1"
                     /translation="MAQIHPTAVVDPSAHLGQNVCIGPFCVVEAGAVIGDGCTLEARC
                     VVKSRVALGRSNVIGEGAVLGGKAQHLHEHQPGGALVIGDNNRLRENVTMHRGWSNDA
                     ATMIGDNNMFMVGSHVGHDCRIGNHCIVVNHVLLGGHVTIEDRAYLGGAAGVHQFCRI
                     GRLAMIGGLAKVTQDVPPFVMVEGGGFTQVVGLNKVGLRRNGYSPADVIQLKEAYRVI
                     YRQGLRWQEVLAILEEQFTTGPAALFYSFLKQGKRGFVQERRISRKATLKLIDPALDE
                     IDDAARDAA"
     gene            4466..5689
                     /gene="eboE"
                     /locus_tag="VFB80_10255"
     CDS             4466..5689
                     /gene="eboE"
                     /locus_tag="VFB80_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HZN34193.1"
                     /translation="MPSPANTYATGYCTNVHAGRTLDETRANLERHALAVKERYSPAG
                     PMGVGLWLSAAAADELIAKHEVERFGQWLAERGLVPYTLNGFPYGDFHQEVVKHRVYE
                     PTWWQPERLHYTMQLVEIQDTILPKGLEGSISTLPIAWGTPCPDRGELEAAAGQLLQA
                     AQWLHKLHEEEGRLIYLCLEPEPGCVFSFADDLVHFLEWQLLRGPDAEIVRQHIRVCH
                     DVCHAAVMFEDQEDVFKKYAAAGIGVGKIQVSAALRMDLDLFEPPAAAPRAQAIKQLA
                     QFAEDRYLHQTVVQRDGDNIFYEDLKLALEAETNEPRGQWRVHFHVPIYLQEFGRLCS
                     TQEQIVQCLAAARKHTTCQHYEVETYAWGVLPAELKQPDLAAGIAEELAWFDKLSKRK
                     VAPWKRDEFGRGART"
     gene            complement(5711..6994)
                     /locus_tag="VFB80_10260"
     CDS             complement(5711..6994)
                     /locus_tag="VFB80_10260"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZN34194.1"
                     /translation="MSRLLITGGRILCPAQRLDRTANLLIEDGKIAQLDAQPRGDDRI
                     INAAGKIVAPGLIDLHTQLREPGCEEDETIETGTAAALAGGYTSIACLPETEPPIDTP
                     AAVEFVRQKAARARNCNVFVVGCVSKNREGNELAEIGSLVEAGAVAFSDGLRPIHNPD
                     VLRRALEYCLMFNKPILSHPEARELSQGGVMHEGRVSLVLGLTGMPPAAEDVMTSRDI
                     RLAEATGGRLHLLNVTSAVSIDLIRRTKSRQVPITAAVCAVHFALTDELLRSFDARYK
                     LNPPLRSQQHVEACLAGLKDGTIDVIASGHSPRAAEKKMQELDLAPFGMSALETTLAL
                     TITRLIEPGHLDWITALAKLSTNPARVLGLKKGTLRPGADADVTIIDPRVRWTVDPAR
                     FCSKSASTPLAGQELHGWASHTIVAGEVRYRVASS"
     gene            complement(7001..>7253)
                     /locus_tag="VFB80_10265"
     CDS             complement(7001..>7253)
                     /locus_tag="VFB80_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZN34195.1"
                     /translation="VQFERQHTRPFPSVREYALLYAMNRERMERAKEDILIMAPGPIN
                     RGVEITPDVADGPHSVILEQVTNGLAVRMAVLWLTMGVN"
BASE COUNT         1326 a         2287 c         2396 g         1244 t
ORIGIN      
        1 aatttggttc ttcggttctt cggttcttgg atttttccgc gcgaggctaa ttcgccgcgg
       61 ggtcgatgtt cacgcggcgg ccttcctggt cgaaacgcac ggtgccatcg accagggcga
      121 agagtgtgta gtccttgccc atgccgatgt tgcggccggc gtggaatttc gtgccgcact
      181 ggcgaacgat gatgttgccg gcgacgaccc gctcaccgcc aaaacgcttg acgccgcggc
      241 gctgggggtt gctgtcgcga ccgttgcggg tcgagccttg acctttcttg tgtgccatga
      301 cgcaaactcc tgtccgctat gcggttacga cacggcgttc ccgccggcga taatcgttga
      361 cctatttgcg aatctctgag tctatcggcc atttctgcag ccggcaatgg gagcgatcgt
      421 cagccgggtc gcgtgtcaag agaaacaatt ccggaaaagc tgcgaccaga tgggaataaa
      481 tgttcccttg tgcgcatcga atcatctaga ggcacgatgc gggcctgcca gcgaaaggcg
      541 atcacggatc gccgcgcacc cgcggctttg ggccgacgcg cgggttatga ccaattctct
      601 aacactaata agtactactt aacggtttcc agcccaatgg ccgcaagtca atgatgcgtc
      661 agtagttgcg gcggagaatc gcagaacacg cgcgtttcgt gcccgccaac cagggggccc
      721 aatcaggcca agtcccggtc ctcgaacagc gtcagcgcca gcagcatcgc gacgctgctg
      781 tagagaatgc aatagaccag cgaccacatc aagtaaacga tcggcactgg cacgcctgcc
      841 gccacgctgg cctggatgtt gaagtggtcc aggacgggca gcacggtggc gatcaggttg
      901 ccaaaaaaga tgaccggcgg cagctgattg gccacgactt gcgattgcac tagcagcggc
      961 gtcaggtggc ccagcacgta aatcgagaag ctgatgatga agttcgccag cgccggcagc
     1021 cgcgtggaga tcgccacgct cagggcggca atgaccagcg tttcgagaaa aaccagcacg
     1081 atgccgggga cgatctgcac catctcgcgg tggcagtcgt gccaggtcgg gtcctggttc
     1141 tgctgctggt ggttgatctc gtagcctcct tcgcggaagt catagatcgg cttgtaggcg
     1201 accgtgacca agagcagcag gccgaatatc gcgcacatca ggccgaccga ccaggcgatg
     1261 ccggtgaact tgccgaggat gaagtcccgg cggctgacgg gcttggacag cacggtgagg
     1321 gcggtcttgc cttcgacttc gtcggcaacc gaagagctgg cggcccacac cgattggaat
     1381 acgcacagca ccaggatcag gctcaggccc gaatccttga gctgcttgat gtcgtcgccg
     1441 aacgtgttgt aggggatgaa tacgaacagc aagagcaaga acacgccgat cgccatgagg
     1501 atggcgtaca gcggctgcgc gatgtcggat ttggccgtcg agagggcgac ggccgccagc
     1561 ttcggcaggg cggcccgctg gagcaggtac aggaagaaga tcgcggccat gcccacggcc
     1621 atgatcggca ccaggaccat ctcgggcgtg gccacttcgc ggatcggagt gatctgcagc
     1681 gtcgccttgg ccaggccttg gtttttggcg tagagcttcg tgatcaccgg ctcgcggaag
     1741 ggcgtcgtgc ccgtctgttc gcggcgccac gttttcgact cgccggcagg gatgtcgagc
     1801 gtcagcacca gcgacgggtc ggtggtcgag cctgtcggct cgctgcggag cttcacgcgc
     1861 tggtcgctct tgaagaccag gccgcgaatc tcgtcgcggc ggaaattgac cggcacctcc
     1921 tgctcgaccg gatcctcgac cggattggcc ggagctgcca ggtcgcgcgt catcgttgcg
     1981 tcaccctcga gcgcgagctg cgggccgcgg accagcgact ccagcagctc cgtcggcttg
     2041 cgaacgatga acagcccgaa cactgcaaag ccggtcatca ccagcgcggt gatgaacagc
     2101 ggccacagca ccccctcgcg aatggcgatc cgcgtttcag ccagcgcccg ccggctgacc
     2161 agcgtgacca gcgcggtggc cagcacccag caggccagcg ctgctccggc caaagcccag
     2221 atgcgatcca gaagcggcgc tgcttgcgcg ttgggcacgt tggtcaggta aagaccgacc
     2281 gccagaccca gcaagaggat tgtcagcacc gcggtcgcca gccgccgcgg ccccggtgca
     2341 tcggccagtg tgccaatgcc gggaagcagc gagagcagct tcagaactcc ccagatggcc
     2401 gccgtgagtg cgaggccggc ggccgcgccg atgcacagaa tccacaccgg gatcagccag
     2461 ttgacgatcg ggtcggccaa ctggatgcgg aagcggtcga agaagtctgc aaacagtagc
     2521 ggcgaggcaa gcaagctcat gggcgaggga ttccgagtag tcggcgagcc aacagccaac
     2581 ggcgtcgccg caagtgcggc ggtacacggc ctttacgatg ctgtcaggtc gttggttcta
     2641 ggccttacag cttattcgcc tagcgggcaa tcgtaaacga cttccactct cctgtcggcg
     2701 gcaggtccgc cgactccgcg ccgcgaatat agcgggccat tgtcgaggca acttttgcga
     2761 cgaaggcctc cacaacgggc ggttctccct cggcaaccag ctccacacga ccgtcggaaa
     2821 gattgcgcac aaacccagtc accgcatagc tttgcgcgat gtgctgggtg gtatagcgga
     2881 agccgacgcc ctgcacgtta ccggcgtaaa gcacccgccg gcggacaatt tcggtaccat
     2941 tcatagctga gcctgccgtc atcacgtgtg aggatgcgaa tcgggctaaa gttgagtcgg
     3001 cttgccgcac gatggtggat ttgcggctaa ccgcacgacc ctggaaatcg ctgcgagagc
     3061 ttatggacca agcggactcg gttgaaatct attgccccaa ttgtcgccag catatcgtgc
     3121 cgctgaaggc gtcggtgagt tggtactgcc cgatctgcgc ctggcagttt agcgatgcgg
     3181 aaatcgaccg gcagggcggc ggccccgctt gcaccaagcc acccgagtcg aagaaggatc
     3241 gcccgcgcga atcgtgagtg gatgccgcta gcagtcgccg tgagcgactc cgccgctcga
     3301 gcagcgaaaa ccgaactcga caagttcccc aaaaacggca taatccgcac cgcgcgtcaa
     3361 aagtgccccc cactcgtggg ccgatttgac ggtaggacac tacccctggc ggccgctgcc
     3421 agcgtccgcc ccgctgcacg aggcagcggc cgggccctta ctacacggac ctgcatcgct
     3481 gccgccggtg ggcatgacgc ccgccgcctg aaaggaccgc cgtggcccag attcatccca
     3541 ccgccgtggt cgatccctct gcgcacctgg gccagaacgt ttgcatcggc ccgttctgcg
     3601 tcgtcgaagc cggcgcagtc atcggcgacg gctgcacgct ggaagcccgc tgcgttgtca
     3661 aatctcgtgt ggcgcttggc aggagcaatg ttattggcga aggcgccgtc ttgggcggca
     3721 aggcccagca tctgcacgag catcagccgg gaggcgcgct ggtcatcggc gataataacc
     3781 gcctgcgcga gaatgtcacg atgcatcgcg gctggtccaa cgacgccgcg acgatgatcg
     3841 gcgacaacaa catgttcatg gtcggctcgc acgtcggtca cgactgccgc atcggcaacc
     3901 actgcatcgt cgtcaaccac gtgctcctcg gcggtcacgt cacgatcgag gaccgggcct
     3961 atctgggcgg ggcggcgggc gtgcaccagt tctgccgcat cggccgactg gcgatgatcg
     4021 gcggcctggc caaggtcacc caggacgtgc cgccgttcgt catggtggaa ggcggcggct
     4081 tcacgcaggt cgtcggtctg aacaaggtcg gccttcgccg caacggctac tcgcctgccg
     4141 acgtgataca gctcaaagag gcctatcgcg tgatctatcg ccagggcctc cgctggcagg
     4201 aagtgctggc gatccttgaa gagcaattca ccaccggtcc cgccgcgctg ttctacagct
     4261 tcctgaagca aggcaagcgc ggcttcgtgc aggagcgccg catctcgcgc aaagccacgc
     4321 tcaagctgat cgatccggcg ctcgacgaga tcgacgacgc ggctcgcgac gccgcctaag
     4381 aggcaggcca gttcaggccc ggccgatcga gtagaatgac ttttcggacg cttgcgtagt
     4441 cagcagcgtc cgcaggagtc gtttcgtgcc cagccccgca aacacctacg ccaccgggta
     4501 ttgcaccaac gtccacgccg ggcggacgct cgacgagacg cgggcgaatc tggagcggca
     4561 cgcgctggcg gtgaaggagc ggtacagccc ggccgggccg atgggcgtgg gcctgtggct
     4621 ctcggcagcg gcggcggacg agctgatcgc caaacacgag gtcgagcggt tcggccagtg
     4681 gctcgcggag cggggcctgg tgccttacac gctcaacggc tttccctacg gcgactttca
     4741 ccaggaggtt gtgaagcacc gcgtgtacga gccgacatgg tggcagccag agcggctgca
     4801 ctacacgatg cagctcgtcg agattcagga cacgattctg cccaagggac tggaggggag
     4861 catttcgacg ctgccgatcg cctggggcac gccctgcccc gaccgcggcg aactggaagc
     4921 cgccgccggg cagctgctgc aagcggccca gtggctgcac aagctgcacg aagaggaggg
     4981 ccgactgatt tacttgtgcc tcgagcccga gcccggctgc gtgttttcgt tcgccgacga
     5041 cctggtgcac ttcttagagt ggcagctgct ccgcggccca gacgccgaga tcgtccggca
     5101 gcacatccga gtctgccacg acgtctgtca cgcggcggtg atgtttgagg accaggaaga
     5161 cgtgttcaag aagtacgcgg ccgctgggat cggcgtcggc aaaattcagg tctcggcagc
     5221 cttgcggatg gacctcgacc tgttcgagcc gccggccgcc gcgccgcggg cccaggccat
     5281 caaacagctc gcgcagttcg ccgaggaccg ttatctccat cagacggttg tgcagcggga
     5341 cggcgacaac atcttctatg aggacctgaa gctggccctg gaggccgaaa cgaacgagcc
     5401 ccgcggccag tggcgggtcc attttcacgt gccgatctac ctgcaggaat tcggccggct
     5461 ctgctcgacg caggagcaga tcgtccagtg cctggccgcc gcaaggaagc atacgacctg
     5521 ccagcactac gaggtcgaga cgtacgcctg gggcgtgctg ccggcggaac tcaagcagcc
     5581 ggacctggcc gccggtatcg ccgaggagct cgcctggttc gacaagctga gcaagcgcaa
     5641 ggtcgctccc tggaagcgcg acgaatttgg ccgcggtgcg cggacctgag ggttcggcgt
     5701 tctagaagtg ctacgagctg gccacgcgat accgcacctc gcccgcgacg atagtgtgcg
     5761 aggcccagcc gtggagttcc tggccggcga gcggcgtgct ggcgcttttg gagcagaacc
     5821 gcgcgggatc aacggtccag cggacgcggg gatcgatgat cgttacgtcg gcgtcggcgc
     5881 cgggcctcag cgtgcctttc ttcagaccga gcacgcgggc cggattcgtc gagagcttgg
     5941 ccagggcggt gatccagtcg agatgccccg gctcgatcag tcgcgtgatt gtcagggcca
     6001 gcgtcgtctc cagggcggac atgccgaagg gagccagatc gagctcctgc attttcttct
     6061 cggcggcccg cggcgagtgg ccgctggcga tcacgtcgat cgtgccgtcc ttgagcccgg
     6121 ccaggcaggc ctcgacgtgc tgctgcgagc gaagcggcgg gttgagctta tagcgggcgt
     6181 cgaacgagcg gagcaattcg tcggtcaggg cgaagtgcac ggcacagaca gcggccgtga
     6241 tcggcacttg ccgcgacttc gtgcggcgga tcaggtcgat gctgacggcg ctcgtgacgt
     6301 tgagcaggtg cagccggccg ccggttgcct cggccaggcg gatgtcgcga ctggtcatca
     6361 cgtcttcggc ggcgggcggc atgccggtca gtccgaggac gagcgagacg cggccctcgt
     6421 gcatcacgcc gccctgcgac agttcgcggg cctccggatg gctcaggatc ggcttgttga
     6481 acatcaggca gtattcgagg gcccggcgga gcacgtcggg gttgtggatc ggccgcaggc
     6541 cgtcgctgaa ggctacggcg ccggcttcga ccagcgagcc gatctctgcg agctcgttgc
     6601 cctcgcggtt tttgctgacg cagccaacca cgaacacatt gcagttgcgg gcccgggccg
     6661 ccttctgccg gacgaactcc acggcggccg gcgtatcgat tggcggctcg gtctcgggca
     6721 ggcaggcgat cgaggtgtag ccgccggcca gcgccgccgc cgtgccagtc tcgatcgtct
     6781 cgtcctcctc gcagcccggc tcgcggagct gcgtgtgcag gtcgatgagg cccggtgcga
     6841 cgatcttgcc ggcggcgttg atgatccgat catcgccgcg cggctgagca tcgagctgag
     6901 caatcttgcc gtcctcgatc agcaaattgg ccgttcgatc gagccgctgc gcgggacaga
     6961 ggatgcggcc accagtgatg agaagtcgag acatgtgtgc ctagttcact cccatcgtca
     7021 gccacaacac cgccatccgc acggccagac cgttggtcac ttgctcgaga atcacgctgt
     7081 gtggaccgtc ggcgacatcg ggcgtgatct cgacgccgcg attgatcggc cccggcgcca
     7141 tgatcaggat gtcctccttg gcccgctcca tccgctcgcg gttcatggca tacaagagtg
     7201 catactcgcg taccgacggg aatggccggg tgtgttgccg ctcgaattgc acc
//
LOCUS       DASRJB010000389         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4056964, whole genome shotgun sequence.
ACCESSION   DASRJB010000389 DASRJB010000000
VERSION     DASRJB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056964"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFB80_10270"
     CDS             complement(<1..372)
                     /locus_tag="VFB80_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN34196.1"
                     /translation="MKLPYNHPGLVVDLGVGLWAWPVPCDADGDGDFDLVVSCPDKPS
                     NGVWLFENATGETAINKLPVFKPARKLSSTAHYVMPSYVAGGLRVLTPGFEHPNFTRT
                     GLAEKVKLPIAARFHQPRGPQT"
     gene            complement(457..786)
                     /locus_tag="VFB80_10275"
     CDS             complement(457..786)
                     /locus_tag="VFB80_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZN34197.1"
                     /translation="MVYSESLADRVRQVFGRRRGLTEKKMFGGVGFLLRGNMSVGIWK
                     SSLIARVGPDAHADALAQPHVREFDITGRPMAGWVLVEPEGIDDDAALRSWIDRAVEF
                     VGTLPGK"
     gene            851..1948
                     /locus_tag="VFB80_10280"
     CDS             851..1948
                     /locus_tag="VFB80_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34198.1"
                     /translation="MTTKLCRFGILGAATIARKNWQAILNSGNATLTAVASRSRERAE
                     QFIQECQASAPFAAPPRICGYDELLASRDVDAVYIPLPTGVRKEWVLKAAAAGKHVLC
                     EKPCGATAAEVAEMIAACERANVQFMDGVMFMHSSRLPAMRKALDDGQSVGRIRWIAS
                     QFSFLGGEEFARKNIRVNSTLEPLGCLGDLGWYNLRFTLWAMGYQTPQRASGRVLTAA
                     RQAGASDVPVEFSGELHFAGGASATFYCSFITQNQQWAVVSGERGALHVEDFVLPFFG
                     SEAAFSISQPQFDVRGCQFNMNERTRRIALPEYSNNAPNSQETNLFRNFAALALGGKP
                     DPHWPRIALQTQQVLEACLKSSRQDGQPVSL"
     gene            complement(2042..2857)
                     /locus_tag="VFB80_10285"
     CDS             complement(2042..2857)
                     /locus_tag="VFB80_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZN34199.1"
                     /translation="MRNPCFFPLLVLNATLTWAADPPVRPTVIGHRGLVQSAPENTLA
                     GFRACLGLRVGFEWDVRRTKDGQLVCLHDETVDRTTSGKGRLADLTLAELRRLDAGRW
                     FDAAFAEERVPQVEEVLEVLARSPPEAGLLAVDLKEAGGGMEEALIKTAQQRVLLGRL
                     IFIGLTIESTDVRRRLKAASREAQTARLAPDEAAVADVLSDRDADWVYVRFLPAAATV
                     ERIHKAGKRVFLAGPLVAGHEPENWKRAAALGLDAILTDYPLELARQLRGRTP"
     gene            2980..3510
                     /locus_tag="VFB80_10290"
     CDS             2980..3510
                     /locus_tag="VFB80_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34200.1"
                     /translation="MVRLLILTAGIALLAGCAKNEQAAPRVQVEHPELGQQFVLAEEP
                     AGAVGILDFREAPPDAAPPTGEVALLGRIGGGQPTWSSQSASFLLSDPAHEIAEGSQH
                     VCTSDNCPFCKAKQGEDKSHAIVMLTLEDNRVPACDARKLLPLEEGQMVVVRGRTEIN
                     ALGQLIVHARGLYVRR"
     gene            3596..3925
                     /locus_tag="VFB80_10295"
     CDS             3596..3925
                     /locus_tag="VFB80_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZN34201.1"
                     /translation="MHRLAACFAACLLVMAAANVTTAQTRVTYTTIVVQDMHCADCAK
                     QIARKLYALPGVVEVRADVPKNIAYVVPQKDKTVSSRAMWEAVEAAGFKVARLEGPQG
                     TFTAKPR"
     gene            complement(3906..4733)
                     /locus_tag="VFB80_10300"
     CDS             complement(3906..4733)
                     /locus_tag="VFB80_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZN34202.1"
                     /translation="MTLAQRVWFALLAIPGATIDRGFWYFVVAGGAWLVLDVVLAKWL
                     SARRIHARQPTAGQMGRELLYSLSSLAVYGLVGGFMVFAVVSGWTPMYFRIERFGWTW
                     FVASIFVVILIHDAYFYWTHRLMHHPRLFRLFHRTHHLSTNPSPWAAYAFSPWEAFVQ
                     AGIAPLTIFTIPLHPLAFSLFMLWQISFNVLGHCGYELFPKWFVRSPLGRILNTATHH
                     AQHHETNRANFGLYFNFWDRLLGTNHARYAERFAEVVDPHPSPLPEGEGARISAASR"
     gene            complement(4758..5210)
                     /locus_tag="VFB80_10305"
     CDS             complement(4758..5210)
                     /locus_tag="VFB80_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34203.1"
                     /translation="MQGRWKAAAILYAVCLGGCGGYSQRANIERVAPVSGTLTFQGKP
                     LEFHQVMFAPEDGRRPAMGVTDAMGKFTLGTNKPDDGAPPGLNKVTIVWVGPPPKNDP
                     GNEVIIEDVSKLPKPKIRIPAKYGNPETSGLTQDVPSGGLSDLKIDLK"
     gene            complement(5243..>5923)
                     /locus_tag="VFB80_10310"
     CDS             complement(5243..>5923)
                     /locus_tag="VFB80_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34204.1"
                     /translation="SASNLAARQLRLPLFTCPSHAPVPTFIITGTQCPAGCGNSTYMQ
                     SLGNNGNYASNNGPFGRRYGARFAEITDGLSNTALFSEILLGPSPGTGTASPGVVPAG
                     GRDDYRVATDLPTATWDTTADSVYNPACDNRGTAAWLYRGKQFYRGVVVTTYYSHTLT
                     PNSKFRDCIRGNAILDRAHLAARSFHPAGVNLVLGDASVRFVANTVNEATWRAVGSKG
                     DGDQIGDY"
BASE COUNT         1124 a         1931 c         1888 g          980 t
ORIGIN      
        1 agtttgcggt ccccgcggct gatgaaacct ggccgcgatc ggcagcttca ccttttcggc
       61 cagcccggtc ctggtgaagt tcggatgctc gaagccgggc gtcagcacgc gcaggccgcc
      121 agcgacatag ctcggcatga cgtagtgcgc ggtgctgctc agtttgcggg ccggcttaaa
      181 gaccggcagc ttgtttatcg ccgtctcgcc cgtggcgttc tcaaacagcc agacgccgtt
      241 cgatggctta tcggggcacg aaactacaag atcgaaatcg ccgtcgccgt cggcgtcgca
      301 cggcaccggc caggcccaca ggccgacgcc cagatcgacg acgaggcccg ggtggttata
      361 gggcagcttc atgagcggcg ttacgcgtgg ctgagcgaga gcagcgcctg ccgccagcat
      421 ccaacacgcg gtgatagcaa cgagtctcat cgcagctcac ttcccgggca aagtgccgac
      481 aaattccacg gcgcggtcga tccagctgcg gagggccgcg tcgtcgtcga ttccttccgg
      541 ttcgaccagc acccagccgg ccatcggccg gcccgtgata tcgaactcgc gcacatgcgg
      601 ctgagcgagg gcgtctgcat gggcatccgg cccgacgcgg gcgatcagcg acgacttcca
      661 gatgccgacg gacatgttgc cgcggagcag gaagccgacg ccgccgaaca tcttcttttc
      721 agtcagcccg cggcggcggc caaagacctg ccgcacgcga tcggccaggg attcgctgta
      781 gaccataagc caccgattga cgccgccgcg gcccgccgcc aacaatcgcc cggcaaagga
      841 gccgcgctag atgacgacaa aactctgccg cttcggcatt ctgggggccg caacgatcgc
      901 ccgcaagaat tggcaggcaa ttctgaattc cggcaatgcc acgctcaccg ccgttgctag
      961 ccgcagccgc gagcgggccg agcagttcat ccaggaatgc caggcgtctg ctcccttcgc
     1021 cgcgccgccg cggatttgcg gctacgacga gctgctcgcc agccgcgacg tcgatgcggt
     1081 ttacatcccg ctgccgacgg gcgtccgcaa ggagtgggtg ctcaaggccg ccgcggcggg
     1141 caagcacgtg ctctgcgaaa agccgtgcgg ggctacggcg gccgaagtag ccgagatgat
     1201 cgcggcctgc gagagggcca acgtgcaatt catggacggc gtgatgttca tgcacagcag
     1261 ccggctgccg gcgatgcgca aggcgctcga cgacgggcag agcgtcggcc gcatccggtg
     1321 gatcgcttcg cagttcagtt ttctgggcgg cgaggaattc gcgcggaaaa atatccgagt
     1381 caacagcacc ctggagccgc tgggctgcct gggggacctc ggctggtaca acctccgctt
     1441 cacgctctgg gcgatgggct atcagacgcc gcagcgggcc agcggccggg tgcttaccgc
     1501 ggcgcggcaa gcgggcgcgt ccgatgtgcc cgtcgagttc agcggcgagc tgcatttcgc
     1561 cggcggcgcc agcgccacct tctactgctc gttcatcacg cagaaccagc agtgggccgt
     1621 cgtcagcggc gagcgcggcg cgctgcacgt cgaagatttc gtcttgccgt tcttcggcag
     1681 cgaggcggcg ttctcgatca gccagcccca gttcgacgtc cgcggctgcc aattcaacat
     1741 gaacgagcgg acccggcgca ttgccctgcc cgaatacagc aataacgcgc ccaactcgca
     1801 ggagacgaac ctgttccgaa acttcgcggc gctggccctc ggcggcaagc ccgatccgca
     1861 ttggccgcgg atcgcactgc agacgcagca ggttctcgaa gcctgcctga agtcgagccg
     1921 gcaggacggc caaccggtaa gcctgtaacg gactcgtcga gcaggcagag accacgaaag
     1981 acaccaaagg tttccctttc gtgtctttcg taaatttcgt ggttgccgct gccccattca
     2041 gttacggagt gcgcccgcgc aattggcgcg ccagctccag cggatagtca gtcaggatgg
     2101 cgtcgaggcc gagcgcggcg gcccgcttcc agttctccgg ctcgtggccg gcgacgagcg
     2161 ggccggcgag gaaaactcgc ttgccggctt tgtggatccg ctcgacggtc gcggccgcgg
     2221 gcaggaagcg gacgtaaacc cagtccgcat cgcgatcgga aagaacgtcc gcaacggcgg
     2281 cttcgtccgg agcgagccgc gcggtctgcg cttcgcggct ggcggccttg agccggcggc
     2341 gcacgtcagt cgattcgatc gtcaggccga taaagatcag ccggccgagc agcacccgct
     2401 gctgcgcggt tttgatcagg gcctcttcca tgccgccgcc ggcttccttc aagtccacgg
     2461 cgagcaggcc ggcctccggc ggcgagcggg cgaggacttc caggacttcc tcgacctgtg
     2521 ggacgcgttc ttcggcgaag gccgcgtcga accagcggcc ggcatcgagg cggcgcagct
     2581 cagccaaggt caagtcggcc agccgcccct tgccgctagt cgtgcgatcg accgtttcgt
     2641 cgtggagaca aaccagctgg ccgtccttcg ttcgccgcac gtcccactcg aagccgacgc
     2701 gcaggccgag gcaggcgcgg aaacccgcga gcgtgttctc gggtgcggac tgaacgaggc
     2761 cgcggtggcc gatcaccgtg ggccgcaccg gcggatcggc ggcccaggtg agggtggcgt
     2821 tcaggacgag cagcggaaag aagcagggat ttctcatggc ggcattctga cggccgcagc
     2881 cggcccagtc cactcgctag cagcaccaga aaaatcaacc ttgccgccgc ggcatgccga
     2941 gactaatacc agcgcgcttc cccgagagaa tctacgccga tggttcgctt gctgatcctg
     3001 acagctggaa tcgcactgct tgcaggctgc gcgaaaaacg agcaggccgc gccgcgagtg
     3061 caggtggagc atccggaact aggccagcag ttcgtgctgg ccgaagagcc ggcgggagcg
     3121 gtcgggattc tcgacttccg cgaagcgccg ccggatgccg caccgccaac gggcgaagtc
     3181 gcgcttttgg ggcgcatcgg aggcggccag ccgacctggt cgagccagtc ggcctcgttc
     3241 ctgctcagcg atccggcgca cgaaatcgca gagggcagcc agcacgtttg caccagcgac
     3301 aactgcccgt tctgcaaagc gaaacagggc gaagacaagt cgcacgcgat cgtgatgctc
     3361 acgctagaag acaaccgcgt gcccgcctgc gacgcccgca agctattgcc gctggaagag
     3421 ggccagatgg tcgtcgtccg cggccgcacc gagatcaacg ccctcggcca gctgatcgtc
     3481 cacgcccgcg gattgtatgt ccgacggtag ggagtttaga ggtcagaggt caagaagttt
     3541 cgaaccctaa cctctaacct ctgacctctg cattctgaaa ttccaggagc cactcatgca
     3601 ccgcctcgcc gcctgcttcg ccgcttgttt gctcgtaatg gccgccgcca acgtgacgac
     3661 cgcccagacg cgggtcacgt acacgacgat cgtcgtccaa gacatgcact gcgcggactg
     3721 cgccaagcag atcgcccgca agctctacgc cctgccgggc gtcgtcgaag tccgggccga
     3781 cgtgcccaag aacatcgcct atgtcgtgcc gcagaaggac aagacggtct cgtcccgcgc
     3841 gatgtgggag gccgtcgaag ccgccggctt caaagtcgcc cgcctggaag ggccgcaggg
     3901 cacgttcacc gcgaagccgc gctaatgcgg gctccctctc cctctgggag agggctgggg
     3961 tgagggtcca ccacttcggc aaaccgctcc gcataccgcg cgtggttcgt gcccagcagg
     4021 cggtcccaga agttgaagta gaggccaaag ttggctcggt tggtctcgtg gtgctgggcg
     4081 tggtgcgtag ccgtgttcag aatccggccc agcggcgagc ggacgaacca ctttgggaag
     4141 agctcgtagc cgcagtgccc caagacgttg aacgaaatct gccagagcat gaacagcgag
     4201 aacgccagcg ggtgcagcgg gatcgtgaag atcgtcagcg gtgcgatgcc ggcctgcacg
     4261 aaggcctccc agggactgaa ggcgtaggcc gcccagggcg aaggattggt cgagaggtga
     4321 tgggtgcggt gaaacaggcg gaacagccgc ggatggtgca tcagccggtg ggtccaatag
     4381 aagtacgcgt cgtggatcag gatcacgaca aagatgctgg ccacgaacca ggtccagcca
     4441 aaacgctcga tccgaaaata catcggcgtc cagccgctga cgacggcaaa gaccatgaac
     4501 ccgccgacca gcccgtaaac cgccaggctg ctgagagagt agagcagctc gcggcccatc
     4561 tggcccgccg tcggctgccg cgcatgaatc cgccgcgccg acagccactt cgccagcacg
     4621 acgtccagca ccagccacgc cccgcccgcg accacgaagt accaaaaccc ccggtcaatc
     4681 gtcgccccag ggattgccag cagtgcaaac cagacacgtt gggccagagt catgcggagc
     4741 gccttccgca acggtcgcta ctttagatcg atcttcaaat cgctcagccc tcccgacggc
     4801 acatcctgcg tcaggccgga tgtttccgga ttgccatatt tggccggaat cctgatcttg
     4861 ggcttgggga gcttcgaaac atcttcaata atcacttcgt ttccgggatc atttttcggc
     4921 ggagggccga cccaaacgat tgtaacctta ttaagaccgg gaggagcgcc gtcgtccggc
     4981 ttattcgttc caagcgtgaa cttgcccatc gcgtcggtca cgcccatggc ggggcgacga
     5041 ccgtcctccg gcgcaaacat cacctgatgg aactccagcg gcttcccctg gaatgtcaga
     5101 gtgccggata ccggcgcgac tctttcgata ttcgctcgct ggctgtatcc gccacagcct
     5161 cccaggcata ccgcatatag aattgcggca gccttccatc tgccctgcat gttcggcctt
     5221 tcctctgcct gatagtgatc gactagtagt cgccaatctg gtcgccgtcg cctttgctcc
     5281 ccacggcccg ccaagtcgct tcgttaacgg tattcgcaac gaatcgaacc gaggcatcac
     5341 ccaaaaccag gttcacgccc gccggatgaa agctgcgggc cgcgaggtgc gcccggtcga
     5401 gaatcgcgtt cccccgaatg cagtcgcgga atttggagtt gggcgttagc gtgtgcgaat
     5461 agtaagtcgt gaccacgacg ccccggtaaa attgcttgcc gcgataaagc cacgcggcag
     5521 tgccacggtt atcgcacgca gggttgtaaa cgctatcagc cgtcgtgtcc cacgttgcgg
     5581 ttggcaggtc ggtagcaacg cgataatcgt cgcggccgcc agccggaaca acgccggggc
     5641 tcgccgtgcc cgtgccaggc gaaggcccca gcaagatttc ggaaaagagc gccgtgttgc
     5701 tcaaaccatc agttatttca gcaaaccgcg ctccataccg cctgccgaac ggaccgttgt
     5761 tgctggcgta gttcccgttg ttgccaaggc tctgcatata agtggagttc ccgcagccag
     5821 cagggcactg ggttccggtg atgatgaatg tcggcacagg cgcgtgcgac gggcaagtga
     5881 agagtggcaa tctcaattgc cgcgccgcga gattgctggc cga
//
LOCUS       DASRJB010000390         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2365618, whole genome shotgun sequence.
ACCESSION   DASRJB010000390 DASRJB010000000
VERSION     DASRJB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365618"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFB80_10315"
     CDS             complement(<1..641)
                     /locus_tag="VFB80_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN34205.1"
                     /translation="MPQLTPVRPQRRRGNISRPVVAIVASLALLAILVVLLFQGPPQP
                     GTTPATTNNTKEGQEPLVVYCAASNKSVLEAIRAEYEKAYGTPLQMQFGASQTLLASL
                     TVSKSGDLYLPADDSYLAMAKEKQLVADEFPLATMQAVVAVPKGNPKKIAKLADLLRD
                     DVRLAQANPDAAAIGKLTRQTLTKSGEWDKLHARTTVFKTTVNEVANDVKVGAV"
     gene            782..>2571
                     /locus_tag="VFB80_10320"
     CDS             782..>2571
                     /locus_tag="VFB80_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34206.1"
                     /translation="MKTALSAIVALGLIAVAASGADWPQLQGDALRSGNAPQAALTMP
                     LGLAGTVPLTDGLYAAPVVAGGKVFVIDGSGVVTALDAQSLRVLWKFATRGGAGNCNN
                     VAAPAAIGKFLHVGTIAGYYYVLDQATGNVVREIDCREPIMASPAAGEDRVYFATLGA
                     QVYAVEPDGKLAWTWDFVKEVVKFGGNRWNGMDWLKERPNRVNWKDHFVCSRDICLVG
                     KTVVMPAGGRTVFVEDAGSTPKLAAVGVIPDYDGAEYPATFGQSADAAGHVYVQWHRR
                     DNAGRVEILKLEGGKVQADFVRGTQTAIHLPGLLSFASVAVRGGDVYRTKPEQGLGLC
                     RHSNQPNADAARAVTSSANVKDDAKPAMLSTAGSICPPVLTKDHAIYGGLDGKLYIVP
                     LAGGEPATFATAFGAPITAPVAVADGRVYVPCEDGYLYVLATGGKEPLPKKDLEVWKV
                     RSPITGQLADAKFDWYTNYGNFSGTNANEQGLAPPLRMRWARRLEGTVKHLPVCGGGR
                     LYTHTAEGQIIAVEQDTGRLLWRRYWPDVYLSFTSPLYHNEKLLVPQAGIKQSKMRCL
                     DAATGKLVWESPFTGSPSWSRQFPPVVHGNV"
BASE COUNT          433 a          848 c          855 g          435 t
ORIGIN      
        1 actgccccga ccttcacgtc gttggcgact tcgttgacgg tcgtcttaaa gacggtcgtg
       61 cgggcatgca gcttgtccca ctcgccgctc ttggtgagcg tctgccgggt gagcttgcca
      121 atcgctgccg cgtcggggtt ggcctgggcc agccgcacgt catcgcggag caaatccgcg
      181 agcttcgcga tcttcttcgg attgcccttg ggcacggcga cgacggcctg catcgtcgcc
      241 agcggaaatt cgtccgcgac gagctgcttc tccttggcca tcgccaggta gctgtcatcc
      301 gccggcaggt atagatcgcc gctcttgctg acggtcagcg aggccagcag cgtctgcgac
      361 gcgccgaact gcatttgcag cggagtcccg taggctttct cgtactccgc gcgaatcgcc
      421 tccagcacgc tcttgttgct ggcggcacaa tagacgacca gcggctcctg cccttccttg
      481 gtgttgttcg ttgtggcagg cgtcgtcccg ggctgtggcg ggccctggaa gagcaggaca
      541 acgaggattg cgagcaaagc cagcgacgcg acgatcgcca cgaccgggcg gctgatgttt
      601 ccgcgtcgcc gctgcgggcg aactggcgtg agctgaggca tggccgacga caaaatcaag
      661 ggcaggacga tcgtgggcgg gagtgcagtg catcctaagt ggctcgatga attcatgcaa
      721 gaattggggt atgctgaggc ggctcactgc gaagctctgg ccaaaactcg gaggattttc
      781 cgtgaagacg gcactttcgg cgatcgtcgc tctgggtctc attgcagtgg ccgcaagcgg
      841 agcggactgg ccgcagcttc aaggggacgc cctgcgatcg ggcaacgcgc cgcaggctgc
      901 cctaacgatg ccgctgggcc tggccggcac agtgccgctc acggatggcc tctacgcagc
      961 acccgtcgtg gcgggcggca aggtctttgt gatcgacggc tcgggcgtcg tgacggcgct
     1021 cgatgcgcag tcgctgaggg tcctgtggaa gttcgcgacc cgcggcgggg cgggcaactg
     1081 caacaacgtc gcggcccccg cggcgatcgg caaattcctc cacgtcggca cgatcgccgg
     1141 ctactactac gtgctcgacc aggcgaccgg caacgtggtc cgcgagatcg attgccgcga
     1201 gccgatcatg gcttcgccgg cggccggcga agaccgcgtc tatttcgcga cgctcggggc
     1261 acaggtctac gctgtcgagc cggacggaaa gctcgcctgg acctgggact tcgtcaagga
     1321 agtcgtcaag ttcggcggca atcgctggaa cggaatggac tggctcaagg agcggcccaa
     1381 ccgcgtgaat tggaaggacc attttgtctg ctcgcgcgac atttgcctcg tagggaagac
     1441 cgtagtgatg ccggcgggcg ggcggaccgt gttcgtcgag gacgcaggca gcacgccgaa
     1501 gctcgcggcc gtcggcgtga ttcccgacta cgacggcgcg gagtatcccg cgacgtttgg
     1561 ccaaagcgcc gacgccgcgg gccatgtcta cgtccagtgg caccgccgcg acaacgccgg
     1621 ccgcgtggaa atcctcaagc tcgagggcgg caaggtgcag gccgatttcg tccgcggcac
     1681 gcagaccgcg attcacttgc cgggcctcct gagcttcgcc tcggttgccg tccgcggagg
     1741 cgacgtctat cggaccaagc ccgagcaggg tctgggtctc tgccggcact ccaaccagcc
     1801 caacgccgat gccgcgcggg ccgtcaccag cagcgccaac gtcaaagacg acgcgaagcc
     1861 ggcgatgctt tcgacggccg ggtcgatctg cccgccggtg ctcacgaagg accacgcgat
     1921 ctacggcggc ctcgatggca agctctatat cgtgccgctg gccggcggcg agccggcgac
     1981 gttcgccacc gcgttcggcg cgccgatcac agcgcccgtc gcggtagccg acggccgcgt
     2041 ctatgtgccc tgcgaagacg gctatctcta tgtgctggcc actggcggca aggagccgct
     2101 gcccaagaag gacctggaag tctggaaggt tcgcagcccc atcaccgggc agctggccga
     2161 cgcgaaattc gactggtaca cgaactacgg caacttctcc ggtaccaacg ccaacgaaca
     2221 ggggctcgcc ccgccgctgc ggatgcggtg ggcccgccgc ctggaaggca cggtcaagca
     2281 cctgcctgtc tgcggcggcg gccggctcta cacgcatacc gccgagggcc agatcatcgc
     2341 tgtcgagcaa gacaccggcc gcctcctttg gcggcgctac tggcccgacg tctatctctc
     2401 gttcacctcg ccgctctacc acaacgagaa attgctcgtc ccgcaggccg gcatcaagca
     2461 gtcgaagatg cgctgcctcg acgccgcgac cggcaagctg gtctgggaat cgccgttcac
     2521 cggctcgcca agctggagcc ggcagttccc gccggtcgta cacggcaacg t
//
LOCUS       DASRJB010000391        18885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4057431, whole genome shotgun sequence.
ACCESSION   DASRJB010000391 DASRJB010000000
VERSION     DASRJB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 18885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057431"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFB80_10325"
     CDS             <1..1545
                     /locus_tag="VFB80_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZN34207.1"
                     /translation="DAYNLDYRIERVLEGLGFAKSTFQQEVAKLSGGQQNRLLLAKLL
                     LAEPDLMLLDEPSNHLDIDATQWLEEFLIASDQAILVVSHDRYFLDRVTNRTLELYQG
                     TVESYPGNFTQYKRLKAERLDVQRRTYEKQVEEIAKMEDFIRRYHFGERHQQAEDRRK
                     KLERIERVPPPREIAAPPMGFPEASRTGDIVLRVERLAKGFPGKPLFSDLSFDVLRGE
                     KWGILGPNGCGKTTLLRCLLGELTPDDGRVIFGSGVKRGYFDQLLSDFDPDMPVLDAV
                     RPPHKEFVEQQRRDLLARFGVTGDMVFQRVGSLSGGERNRTALARLSASDANFLLLDE
                     PTNHLDLWARGALERALREFDGSVLFVSHDRYFLNQVATKLLIVEPRSDGSGEMRFRV
                     IDGNYDTYQHFVKQGLAREARASVVAETAAAKPAPASASGESAGKPPKERRKRKFPYR
                     KVAEIEAEIAEREARIESLHQQFGAEEVLRDGARIKELKSELAEHETALPRLYEHWEE
                     ATELNG"
     gene            1738..2097
                     /locus_tag="VFB80_10330"
     CDS             1738..2097
                     /locus_tag="VFB80_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34208.1"
                     /translation="MICNSQHWRAWQRSILVGLLSCLLAVPAGSVSSSTSRVMLPVLP
                     QPTEEESQPRQTPTEEESQSVSCPVSREDWLERQHPPAMVPLLVHHSTAGELGTLCFP
                     RPAELSSRNGCGGPLRC"
     gene            2236..2931
                     /locus_tag="VFB80_10335"
     CDS             2236..2931
                     /locus_tag="VFB80_10335"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZN34209.1"
                     /translation="MEKLIAGVHRFRRLEYPAQQDFFQQLANKDQKPVALFITCADSR
                     VHPNLITQTEPGDLFLIRNAGNIVPPHGAPTGGETATIEYAVEVLGIRNIIICGHSQC
                     GAMQALLGNGSLDHLPAVKAWCAHAEATRRIVRQKYRDLSGHELAVAATEENILVQMN
                     NLSTHPCVAARLASDELHIFGWYYDIGCGLVLQYVQHEGRFVEINDEAHLAHPRRNVP
                     SPGQRLALQGGLR"
     gene            2928..4916
                     /locus_tag="VFB80_10340"
     CDS             2928..4916
                     /locus_tag="VFB80_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN34210.1"
                     /translation="MTDEELQGGRMSTDGAVPAGGWRQDALASLVVFLVAMPLCMGIA
                     LASGAPVAAGLVTGIIGGLIVGCLAGSPLQVSGPAAGLTVICGEVIREHGMQALGVVV
                     LFAGALQFAAGLFKLGQWFRAVSPAVIHGMLSGIGVLILSSQIHVMVDDRPQGSGLAN
                     LISIPEAVVKGLPLPVLEPAERQAERTQLLKQFGQLHERQAEVQSHVGRIITRHGSER
                     RHEWERDHLADFLPPQQALVKDLRAAQQAAEQSKSGLGGDRAEVLAAAVTRAVQSAEA
                     ALADLEQQRLETVPASQAQATQALVAVGASLKSHDWAAKVGLLSILIIMMWQALTPNW
                     MRLIPGPLLAVLACTGVAWWLALPVLYVEVPDNLIDGLTFPSLSVLQDVAPRPLAIAA
                     LMMAVVASAETLLCAAAVDRIHTGPRTQYDRELSAQGVGNMLCGLVGALPMTGVIVRS
                     AANVQAGASSRLSAILHGVWLLVFVAAMGFVLRMIPTACLAGILVYTGFKLIDFKGFQ
                     RLWKYSRTEAAIFAVTLVVIVVEDLLLGVITGVVLSAVKLLISFSHLDVQLTTSGDRH
                     KHPQVTLTIAGAATFLRLPVLAARLEEVPQGAHLHVDFEHLDYIDHACLELLMTWAKQ
                     HEMQGGRLVIDWGQLHARFREVRRGPPRAGPRKLAHSQ"
     gene            4996..6648
                     /locus_tag="VFB80_10345"
     CDS             4996..6648
                     /locus_tag="VFB80_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN34211.1"
                     /translation="MNDAYRPQGNVAPPLAAPTSFWAHLRYFCNPIDARYEELRRDSW
                     YLVVLRDFSAGLIVAMMAIPLAMGFAMASGLKPVQGIVGGAVAGLIGALFGGSKYQVY
                     GPTAAFIPVIAGLMLVYTPLYGFEAAHGTLVLAAMVAGVILLIMGLARLGRLARLVPH
                     SIVVGFTIGIAVTIALSQVGEVLGLHAGFKLTEHELVELEKKSVAPAALKKLHNLTGR
                     EFATRTLFLKELGTVLAPDELKTSRAAILKEAELRMPPHFFEKLRIIAAHRREFKGDA
                     LLLALVTFLITHHLLKISIYIPAPLIAIGCGIGLSHALHDPELTTIGGKYGVIPTEFF
                     VLTPPQLPSWEWTVLRDVGYLVAAIVFVSGVESLLCSRMADRLADNRGTPFDPNKEFW
                     GQGLVQVVVPLLNGFPHTGALARTATNIKLGAMTPLAGIFKCVLKLLLAFFLAGLLAL
                     VPMACIGGILMWVAFNMVKPAEVKQVLAHNWFHIGLMVLTAIAVIITDFLTGVLTGMI
                     AYALLFKFLDTPSQQQSNSGGPDDTVVEDEAAAAEPRQPVVA"
     gene            6694..7599
                     /locus_tag="VFB80_10350"
     CDS             6694..7599
                     /locus_tag="VFB80_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN34212.1"
                     /translation="MHHFRNLLVGLSRNTADAGLIRYAAMIGRWGAAQSIRFVHVLPG
                     AQPAKDESEKAQALNDLQSAVLAHFADVPSTIDRQFDVQSGPLTDCLLAQAAQQRIDL
                     LLVGRHSNGSGRRSLARRLAMKAPCSVWVVPEDFPAAVRRILAPIDFSEPAADTLRTA
                     IALARLCGHPECLALHIYFDQAVTTYEGYDQVLRGEEAQAYRQFIAPIDCQGVKVTPL
                     FEESANVAHAIERVAAREGADLIVMGTRGRSASAAILLGSVTEETIIDTRLPLLAVKH
                     FGAQMGVLQALLDRRFLRSPGLHTD"
     gene            7835..8401
                     /locus_tag="VFB80_10355"
     CDS             7835..8401
                     /locus_tag="VFB80_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34213.1"
                     /translation="MSSPGSHPDEVDQLLLNAKLRDELEPFLDESVHILDCRRLPTDA
                     ENEFLAQMLAWERAPVLPISQWFTPELTLPAPDKLNERELRKLLTATIEQLYSRRIVL
                     DFTDHLSDRQLYCLIARDILPSPEKKLDLPRNYLHWHFLDVEAQPETWLSFYASDEER
                     DAWAAETGQPLPPRRTPPYPRKMPRRPS"
     gene            8486..9265
                     /locus_tag="VFB80_10360"
     CDS             8486..9265
                     /locus_tag="VFB80_10360"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZN34214.1"
                     /translation="MPKLLAPDQSRSFEEHGIAFPVRVLSADEAFAYRAQCDALEAQL
                     GGKPRTVEVRQMHLHFQWAWRLATHERVLDAAEDLLGPNLLIWATELFAKHPRDNVVS
                     IGWHRDGPYMGLAPERTVTAWIALTESRQANGCMQVVREASRQEKRNRQPAASDVIDE
                     VSLAAGEMSLHDVFVLHGSGPNLSAEKRVGFAIRFTTPEGRPHAERPPAILARGSDRY
                     GYFRLLQPPREDDAEAALAQMRGSARAHLDATLENLQRARR"
     gene            9278..9529
                     /locus_tag="VFB80_10365"
     CDS             9278..9529
                     /locus_tag="VFB80_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34215.1"
                     /translation="MAEIEAAVRDYILTAFLTGARGAALRNDDDLLMVLDSLQMLRML
                     IDMEAQFSIRVDNSEMLPENLGTIEKLAAFIARKRQAAG"
     gene            9526..10755
                     /locus_tag="VFB80_10370"
     CDS             9526..10755
                     /locus_tag="VFB80_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN34216.1"
                     /translation="MNADADVVVCGAGAAGLAAALAAARSRASVLLVERSPQIGGTVA
                     HALIHTLGGLYDAYGNVQNAGLPAELIDRLHEADPRTRRRRLGRTWVLNVCPNVYRAA
                     VTRWIAEQPGINVMCQSQASRVVRRANRIFELEVTSHAHVLRLRPRAVIDATGTAEMV
                     RLLDPALVSDEESRASGGWIFRLSGVAAGALDFPKGLAAVRALRSAAAAGELPPLSHH
                     AWLDFGVDTDEVFVKLMVPLQRDWQDEAVETARRAGEAQAAIVHFLRRFPDFAQAEVT
                     QSGSLGVRDGGRIRGRYTLTAEDVRRGRKFAEAAGRCSWPIEYWDAERGASVEYLTEG
                     TEYDIPQAALQVWGVDNLWACGKCLSADRWAHASARVVGACWAMGQAAGIAAAACSPH
                     PGQELSNQHEQPLPAIS"
     gene            10727..12166
                     /locus_tag="VFB80_10375"
     CDS             10727..12166
                     /locus_tag="VFB80_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN34217.1"
                     /translation="MSNLYQRFRETAARRPEHLAIVGPRGARWSSADLLAAMDAAGDE
                     LHAAGVRPGACVGLHATSSAQYIIDTYAIWRCGGCVVPIPTELAIGEKAEICRAIALD
                     YVLSPPASAEFLDESRRGKSWQLGERALVEWRPAREHPSGFRELDAGFIRFTSGTTGS
                     SKGVVLSHTTIGERIAGANEALRLSPADRVLWVLSMSYHFTVSIVAYLTYGATIVLPA
                     NHLAPAILSAIREHRATILYASPLHYALLADYLEAAPLASLRLAISTTSPLDERIARA
                     FAQKFGRPVSQALGVIEAGLPCIHTDATANRWASVGRVLPPYRLKLADTGLSDGLREV
                     LLGGPGFLDAYYDPWQPRSAIMPDGWFRTGDVGRVDDDGYLFLTGRSKDVISVMGMKF
                     FPQEVERVLVIHPQVRGASVFAVKDPRWGERAMARVVAAADAPGEALAEELRRHCQQS
                     LAAYKVPERIEFVPALPRTASGKILHRST"
     gene            12179..13540
                     /locus_tag="VFB80_10380"
     CDS             12179..13540
                     /locus_tag="VFB80_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34218.1"
                     /translation="MKPNPDRQLLNPLDVALRCIDHAIRGMGFPGFETQMLVALAERV
                     DVPRLRNALTRFGRRHPVVTARLVEDEGRHPSFWQFRPGAIPQLPEIELSSAEPSAVL
                     AAAAELLSTPQDPAAADPLRFHLLHRPGGGDVLVIQYNHTLIDNSATALIVREIDGLS
                     QTGGTAEEDQPQFEPRFLIGNYLRHVPHADRRAAAQAAIELQAHIIRSRGALLGRGEK
                     NVTRPPRLKVAARVAEPELTRAIRGRAAELCGLPSLSMAMLGSAFRGIRALGSPEHNT
                     GRHYNAGIGLDLDLREESGALLQNLLSLVAIYARGDELADRDGLVRSLSLQMRDRLVR
                     RLDLGIVRLVHVFHRRPRYVSWVMGHLLRWSCSVWYAYFGALDSVGPRFCGVPVERVQ
                     YIGPTWSPKGIALLVDQFAGRLYLQLTFDPELVAEPLANTYLDWLMGDLEEFAAASPP
                     RSL"
     gene            complement(13518..14510)
                     /locus_tag="VFB80_10385"
     CDS             complement(13518..14510)
                     /locus_tag="VFB80_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HZN34219.1"
                     /translation="MRAPIVSLLVCLVAVSPAGAWNATGHKLISSLAFRQLSAADQGR
                     VVAILQRHPRFTPDFADEMPPEVKAGDEAMQNEWLFQQAGIWADIVRGGPPERTAFHR
                     PTWHYVDLPHFLNDTTRADLDGQIKENVALDPPPTATPELQEMNVTQTIRLARRLAAD
                     KQAAPETRALMLAWLFHAVGDLHQPCHSTQLYSNRLFPEGDQGANKIKLVQSFNLHAL
                     WDGFLGQDAEFRAVRNRAIAMSADAKWMKLGQEAAADLDAKSWRDESHLVAVTVVYDA
                     ELMIALRKVEASADPPEPLTLSEDYLRMGGRNAERRVVQASYRLAAVLKAIVAD"
     gene            14651..17932
                     /locus_tag="VFB80_10390"
     CDS             14651..17932
                     /locus_tag="VFB80_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN34220.1"
                     /translation="MNRGLLAALVLLAAGAVCAEEPRAALDYFEREVRPILVDHCQKC
                     HGAGKQESDLRLDSREALLKGGVSGPAIVPGQPGKSTLIAAIRHEGDIKMPPKSKLTE
                     AQIAAVTHWVASGAVWPSAIAVAKSGDSRLTHWAFQPVRDPPVPVVAGDQSANPIDAF
                     VLEKLAAAGLSASPPADRRTLIRRMTVDLTGLLPTAEEVEAFERDSDPKAAEALLDRL
                     LASPHYGEQQARHWLDVARYSDTKGYVYGREQRFWLHAWVYRDWVVRAFNDDLPYDRF
                     LLLQIAADQAAPDDKAALSAMGFLTLGRRFLGVQRDIIDDRIDVVTRGTMGLTVGCAR
                     CHDHKYDPIPTRDYYSLYGVFQSSAERSVTIAEPAQPDEAYQKFAEELEKRRKNLADG
                     LAARRLETADHNRSRTGDYLAAQLELHNYPPEGFDQIFSKTDLLPTFVHRWEAYLADA
                     KKRSEPIFVPWHAYSEIPARDFATKAAAVTEQLNSRPDGAINPLVAGKFAAAPASMAE
                     VAARYGELFAEIEAQWKKSAPAPLADPAAEALRQVLYSRAGPCEVPDEPIVNIEYFVD
                     SGSCDALWKLQNEVDNWILQGPPSMRMAVILTDRNPPIEPHVFRRGNPVNLGEEIPRQ
                     FVEVLAGPDRRPFASGSGRLELAQAIIDPKNPLTARVIVNRIWAHHFGSGLVRTPSDF
                     GTRADPPSHPKLLDWLASRFIEDGWSITKLHRRIMTSRTYQQASSAERGAGSAEQGVP
                     SSVDPENRLLWRQNPRRLSFEEMRDALLAAGGQLDRTLGGRAGDLFARTFVRRSLYGT
                     IDRQFLPSTLRMFDFANPDLHIPLRSETTVPQQALFFLNHPLLIGYAQTVAKATASAA
                     TDEERVRQLFRRAYQREATADEIHSALALISAAAEGPQTAIPVTATAWQYGYGAFDEK
                     MQRVTGFKKLPHFTGTAWQGGSGYPDGKLGWLQLTAEGGHPGNDLAHAVVRRWTAPRD
                     MQVRVTSTLIHEPKEGKGVRGFLVSSRGGLIKQVAVHNGREELNADQLDLQAGDTLDF
                     VADMGDLLSHNQFLWKAQITAAGSGGLTFDSQRDFGDQPTPKLTAWEQLAQVLLSANE
                     FVFVD"
     gene            18026..18310
                     /locus_tag="VFB80_10395"
     CDS             18026..18310
                     /locus_tag="VFB80_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34221.1"
                     /translation="MRYRLRTLMIALGMGPPVIAGVWWGVAWTVAWLVSRPGPHLFVL
                     VSAVAVLYLAAAAVAVAMKELRGAKRFDWLMIAAGIGAALAGLGVIALLK"
     gene            18490..>18885
                     /locus_tag="VFB80_10400"
     CDS             18490..>18885
                     /locus_tag="VFB80_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34222.1"
                     /translation="MQPAAFVSRRDMLRRAGTGLGMLGLASLMADDGLLAAEAGPLTP
                     KHPHFAPRAKRVVHFFCNGGPSHVDTFDPKPALARHAGQPLPQTLRTERKTGAAFPSP
                     FKFQKYGESGLEISDIFARTAEHADSLAVI"
BASE COUNT         3186 a         6170 c         6167 g         3362 t
ORIGIN      
        1 gacgcctaca atctcgacta ccgcatcgag cgggtgttgg aagggctcgg ctttgcgaag
       61 tccacattcc agcaagaggt cgccaagctt tccggcggcc agcaaaatcg cttgctgctc
      121 gcgaaattgc tgttggccga gccggacttg atgctgctcg acgagccgtc gaaccacttg
      181 gatatcgacg cgacgcagtg gctggaagaa ttcctgatcg cgtcggacca ggcgattctg
      241 gtcgtcagcc acgaccgcta tttcctcgac cgcgtgacca accgcacgct ggaactgtat
      301 caaggcaccg tcgagtcgta tccgggcaac ttcacgcagt acaagcggct aaaggcggaa
      361 cggctggacg tgcagcggcg gacctacgaa aagcaggtcg aggaaatcgc caagatggaa
      421 gatttcatcc gccgctacca ctttggcgag cgccatcagc aggccgagga ccgccggaag
      481 aagctggaac ggatcgagcg cgtgccgccg ccgcgcgaga tcgcagcgcc gcccatgggg
      541 tttccggaag cgtcgcggac cggcgacatc gtgcttcgcg tcgaacgcct cgccaaaggc
      601 tttcccggca agccactctt ctcggacttg tcgttcgacg ttctccgcgg cgagaaatgg
      661 ggcattctcg gccccaacgg ctgcggcaag acgacgctgc tgcggtgcct gctgggagag
      721 ctgacgccgg acgacggccg tgtgatcttt ggcagcggcg tgaagcgcgg ctacttcgac
      781 cagttgctta gcgacttcga tcccgacatg ccagtgctcg atgccgtgcg gccgccgcac
      841 aaggagttcg tcgagcagca gcggcgcgac ttgctcgccc ggttcggcgt taccggggac
      901 atggtctttc aaagagtcgg cagcttgagc ggcggcgaaa ggaaccgcac ggcgctggcc
      961 cgcttgtcgg catccgacgc gaacttcctc ctcctcgatg agccgacgaa ccatctggac
     1021 ctgtgggccc gcggcgcgct cgaaagggct cttcgcgagt tcgatgggtc ggtgctcttc
     1081 gttagccacg accgctactt cctgaaccag gtcgccacga agctgctgat cgtcgagccg
     1141 cgcagcgatg gcagcggtga aatgcgattc cgcgtgattg acggaaacta cgacacgtac
     1201 cagcatttcg tgaagcaggg tctggcccgc gaggcccggg cgagcgtcgt cgccgaaacg
     1261 gcggcagcga aacccgctcc ggcatcggcc agcggagagt ccgcgggaaa gccacccaag
     1321 gagcgccgca aacgcaagtt tccataccgc aaggttgcag aaatcgaggc tgagattgcc
     1381 gagcgcgaag cccggatcga atcgctgcat cagcaattcg gcgcggaaga ggtactacgc
     1441 gacggggcgc gaatcaagga gctaaagtcc gaactggccg agcacgaaac cgccttgccg
     1501 cgactctacg aacactggga agaagccacc gaactaaacg gctagagccc ggacgaatcc
     1561 ccctcaattg ggaataagca acacgttttt ggctgtgatt tgatgtaaaa gtcggcaatt
     1621 tgatgaaaaa ggggctggcc gggcattttc ccggttgccg acaacataac aggttcgtaa
     1681 tctttcgact gagttccgca tgaggacctt cttttaactg tcttgcggcg attttcaatg
     1741 atctgcaact cccagcactg gcgcgcttgg cagcgcagca tcctggtggg attgctgtcc
     1801 tgcctgctcg cggtgccggc gggatccgtc agcagcagca cctcgcgcgt gatgttgccg
     1861 gtgttgccgc agccgacgga agaagagtcg cagccccgcc agacgccgac cgaagaagag
     1921 tcgcagtcgg tgtcctgccc cgtcagccgg gaagactggc tggagcggca acacccgccg
     1981 gcgatggtgc cactcttggt gcatcattcg accgcgggtg agctgggaac gctctgcttt
     2041 ccacggcccg cggaattatc gagccgcaac ggctgcggcg gaccgctgcg ctgctaggcg
     2101 gcgcattctg cccgcgccct tctcgcgcta gtcatccgcc cttgtgtcgt cgttctctgc
     2161 atcgcttctt tgagcgctct gccgctccgc gaactgatgt gcctggcaac cgccgcactt
     2221 gtcctcgagg aacccatgga aaaactcatc gccggcgtgc accgtttccg ccgcctggaa
     2281 tatcccgccc agcaggactt ttttcagcaa ctggccaaca aagatcaaaa gcccgtagcc
     2341 ttgttcatca cctgcgccga ttcgcgcgtg catcccaacc tgattacgca gacggagccg
     2401 ggcgacctgt ttctgatccg caacgccggc aacatcgttc caccgcacgg cgcgccgacg
     2461 ggcggcgaga cggcgaccat cgagtacgca gtcgaagtgt tgggcattcg caacatcatc
     2521 atctgcggcc actcgcaatg cggcgcgatg caggccttgc tcggcaacgg gtcgctcgac
     2581 cacttgccgg ccgtcaaggc ctggtgcgca cacgccgagg ccacgcggcg gatcgtgcga
     2641 caaaagtacc gcgatctgtc gggccacgag ctggcggtgg cggccacgga ggagaacatc
     2701 ctcgtgcaga tgaacaatct cagcacccat ccctgtgtgg cggcccgtct ggcgtccgac
     2761 gaattgcaca tttttggctg gtactacgac attggctgcg ggctggtctt gcagtacgtg
     2821 cagcacgagg gccggttcgt cgaaatcaat gacgaggccc accttgccca tccgcggcgc
     2881 aacgtgccgt cgccgggcca gcgactcgca ctccaaggag gcctgcgatg acggacgagg
     2941 agctgcaggg gggcaggatg tccacagacg gcgccgtgcc ggccggcggc tggcgacaag
     3001 atgcgttggc gtcactggtg gtatttctcg tggccatgcc gctctgcatg ggcatcgcgc
     3061 tcgcgtccgg cgctcccgtc gcagcgggcc tcgtgacagg catcattggc ggcttgatcg
     3121 tgggctgttt ggcggggtcg ccgctccagg tcagcggccc ggcggcgggc ctgaccgtca
     3181 tttgcggcga agtgatccgc gaacacggca tgcaggcgct cggtgtcgtc gtcttgtttg
     3241 ccggtgcact gcagttcgcc gctggactgt tcaagttggg ccagtggttt cgagccgtct
     3301 ctcccgccgt gattcacggc atgctttcgg gcatcggcgt gctgatcctg tcgagccaga
     3361 ttcacgtcat ggtggacgat cgcccgcagg gcagcggcct ggcaaacctg atttccattc
     3421 ccgaagccgt cgtcaaaggc ctgcccctgc ccgttctgga gccggccgag cgccaggccg
     3481 agcggacgca gctgctgaag cagtttggtc aactccacga gcggcaggcc gaggtgcaat
     3541 cgcacgtcgg gcgaatcatc acccgccacg gcagcgagcg ccgccacgag tgggaacgcg
     3601 accacctggc cgattttctg ccgccgcagc aagcactcgt taaggacttg cgtgccgcgc
     3661 agcaggccgc cgagcagtcg aagagcggcc ttggcggcga ccgggccgaa gtcttggcgg
     3721 cagccgtgac gcgcgccgtg caaagcgccg aggcggccct cgccgacctc gagcagcagc
     3781 gtctcgagac cgtcccggcc tcgcaggcgc aggccacaca ggctctcgtc gcggtggggg
     3841 ccagcctgaa aagccacgac tgggcggcca aagtcggcct gctctcgatc ctgatcatca
     3901 tgatgtggca ggcgctgacg cccaactgga tgcggctgat ccctggcccg ctgctggccg
     3961 tgctggcttg caccggcgtg gcttggtggc tggcgctgcc ggtgctgtac gtcgaggtgc
     4021 cagacaatct catcgacggc ctcacctttc cgtcgctcag cgtgcttcaa gacgtggccc
     4081 cgcgcccgct ggcgatcgca gccctcatga tggcggtagt ggccagcgcc gagacgctgc
     4141 tctgtgccgc cgccgtcgat cgcatacata cgggaccgcg gacgcagtac gaccgcgagc
     4201 tgtcggccca gggcgtcggg aacatgctct gcggcctggt cggcgcgctg ccgatgaccg
     4261 gcgtgatcgt gcggagcgcg gccaacgtcc aggccggcgc ctcgtcgcgc ctgtctgcga
     4321 tcttgcacgg cgtgtggctc ctcgtctttg tggcggccat gggcttcgtg ttgcggatga
     4381 ttcccacggc ctgcttggcg ggaatcctgg tctatacagg cttcaagctc atcgacttca
     4441 aaggcttcca acggctctgg aaatacagcc gcaccgaagc ggccatcttc gccgtcacgc
     4501 tggtcgtgat cgtcgtcgag gatttgctgc tgggcgtcat caccggcgtg gttttgtcgg
     4561 ccgtcaagct gctcatttcc ttctcgcatc ttgacgtgca gctgacgacg tcgggcgacc
     4621 ggcacaagca cccgcaggtg accctgacca ttgcgggtgc cgcgacgttt ctgcggctgc
     4681 ccgtgctggc ggcccggctg gaggaggtgc cgcagggagc gcacttgcac gtcgatttcg
     4741 agcatttgga ttacatcgac cacgcttgcc tggaattgtt gatgacgtgg gccaagcaac
     4801 acgagatgca aggaggccgg ctggtaatcg actggggtca gcttcacgcg cggtttcgcg
     4861 aggtccgccg cgggcctccc cgagccggac cgcggaagtt ggcacattct cagtaaccat
     4921 ctgctggctg cgatcggaac gtatagcgcg ttcgccgagc gctcgtgtgg caccatcgtc
     4981 aacgcgaggt ccgttatgaa cgacgcctat cggccgcagg gcaacgtcgc ccctcctctc
     5041 gccgcgccca cgagcttctg ggcccacctg aggtacttct gcaatcccat cgatgcccgc
     5101 tacgaagaat tgcggcggga ctcctggtac ttggtggtgc tgcgcgattt cagcgccgga
     5161 ctgatcgtgg cgatgatggc gattccgctg gcaatggggt ttgcgatggc ctcgggcctg
     5221 aagccggtgc agggcatcgt cggcggcgcc gtggccgggc tgatcggggc cttgttcggc
     5281 ggctccaagt accaggtgta tgggccgacc gcggcgttca tcccggtgat cgccggcttg
     5341 atgctggtct acacgccgct gtacgggttc gaagccgcgc acggcacgct ggtgctggcg
     5401 gcgatggtgg ccggggtgat cctcttgatc atgggcctcg ccaggctggg gcggctggcc
     5461 aggctcgtgc cgcactcgat cgtggtcggg ttcacgatcg gcatcgcggt gacaatcgcg
     5521 ctcagtcagg tcggcgaggt gctcgggctg cacgccgggt tcaaactaac cgaacacgag
     5581 ctggtggaat tggaaaagaa aagcgttgcg ccggccgcgc tgaaaaagct ccacaacctt
     5641 accgggcgcg agtttgccac tcggacgctg tttctcaagg agctgggcac ggtgctcgcg
     5701 cccgatgagc tgaagaccag ccgcgcggcg atcctgaagg aggccgagct gcgcatgcca
     5761 ccgcacttct tcgagaagct gcggatcatc gcggcccatc gtcgcgagtt caaaggcgac
     5821 gccttgctgc tggcactcgt gacctttctg atcacccatc acctgctcaa gatctcgatc
     5881 tatatccctg ctccgttgat tgccatcggc tgcggaatcg gactctcgca cgccctgcac
     5941 gacccggagc tgacgacgat cggcggcaaa tacggcgtca ttcccacgga attcttcgtc
     6001 ctcacgccgc cgcagctgcc ttcctgggaa tggacggtgc tccgcgacgt gggctacctg
     6061 gtggcggcga tcgtgtttgt ctccggcgtc gagagcctgc tctgttcgcg gatggccgac
     6121 cgcctggccg acaaccgcgg cacgccgttt gacccgaaca aagagttctg ggggcagggc
     6181 ctggtgcaag tcgttgtgcc gctgctcaac ggctttccgc ataccggcgc cctggcccgc
     6241 acggcgacga acatcaagct cggcgcgatg acgcccttgg ccggcatctt caagtgcgtg
     6301 cttaagctgc tgctggcttt tttcctggcc ggcctcctgg cgctggtgcc tatggcctgc
     6361 atcggcggaa tcctgatgtg ggtcgccttc aacatggtca agcccgccga ggtgaagcag
     6421 gttctggcgc acaattggtt ccacattggc ctcatggttc tgaccgcgat tgccgtcatc
     6481 atcaccgact ttttgaccgg cgtgctgacc gggatgattg cctatgccct gctgttcaag
     6541 ttcttggata ctcccagtca gcagcagtcc aattcaggcg gtccggacga caccgtggtc
     6601 gaggacgagg ccgcagccgc cgagccgcgc cagccggtgg ttgcctgacg cgcggatcga
     6661 gcggaaacgc tggcccctgc cggaggatcg ccaatgcacc attttcgcaa cctcctggta
     6721 ggcctgtcgc gaaacactgc cgacgcgggg ttgatccgct acgcggcaat gatcggccgc
     6781 tggggcgcgg cccagtccat ccgcttcgtc cacgtgttgc ccggcgccca gccggctaag
     6841 gacgaatccg agaaggctca agcgctcaac gatttgcagt cggccgtcct ggcgcacttt
     6901 gccgacgtgc cgtcgaccat tgatcggcag ttcgacgtgc aaagcggtcc gttgaccgat
     6961 tgcctgctgg cccaggccgc ccagcagcga atcgacctgt tgctggtggg gcgccatagc
     7021 aacggttccg gccgccgctc gctggcccgc cgtctggcga tgaaagcgcc ctgttccgta
     7081 tgggttgtgc cggaggattt tcctgctgcc gttcggcgaa tcctggcgcc gatcgacttc
     7141 tccgagccag cggccgatac gctgcggacg gccatcgcgc tggcccgcct gtgcgggcac
     7201 ccggagtgcc tggccctgca catctatttc gatcaggcgg taaccacgta cgaaggctac
     7261 gatcaggtgc tgcgcggtga agaggcccag gcgtaccgcc agttcatcgc accgatcgac
     7321 tgccagggcg tcaaagtcac accgctcttc gaggaaagcg ccaacgtcgc acacgccatc
     7381 gagcgggttg ctgcgcgaga gggcgctgat ctcatcgtga tgggcacgcg cggccgcagt
     7441 gcgtccgcgg caatcctgct gggcagcgtc accgaggaga cgatcatcga taccaggctg
     7501 cccttgctcg cggtcaaaca cttcggggcc cagatgggcg ttctgcaagc cctgctggac
     7561 cggcgatttc tccgctctcc gggcctgcac accgattgaa tcagccaact tccgcggtgc
     7621 gtaaaggttc tctagcgtac tgtaaagccg gctgagcgag tctgggcaag ctgtctgcga
     7681 cctggcggat cgtatcgctt ctaccgcgat tgccctgaaa aaagctgcga aatgcggcct
     7741 ttcggaagcg gattttctct tgcggcagca aggcgtccgg ataaagtcga atcagcgctc
     7801 ggattgcaat cgcagcctgg aaacgctccg cgtcatgtcg tctcctggat cccatcccga
     7861 cgaggtcgat cagctcctgc tgaacgccaa gctccgcgac gagctggagc cgtttctcga
     7921 cgaatcggtt cacattctcg actgccgccg cttgccgacg gacgccgaaa acgagtttct
     7981 ggcccagatg ctggcctggg agcgagcgcc ggtgctgccg atcagccagt ggttcacgcc
     8041 cgagctgacg ctgcccgcgc ccgacaagct gaacgaacgc gaactgcgca agctactgac
     8101 cgcgacgatc gagcagctct attctcggcg gatcgtactc gacttcacgg atcacttgag
     8161 cgaccggcag ctctactgcc tgatcgcccg cgacattctg ccgtcgccgg aaaagaagct
     8221 cgatctacct cgcaattatc tgcattggca cttcctcgac gtcgaggccc agcctgaaac
     8281 gtggctgtcg ttctatgcct ccgacgagga gcgcgacgcc tgggccgccg aaaccggcca
     8341 gccgctgccc ccgcgccgca cgccgcccta tccgcgaaaa atgccccgcc ggccgtcgta
     8401 gctgccaatt tgaagccggc aagttgcatt tgcgctagcg cgcgaacata acgtaagcaa
     8461 agccgcgtgt cagccggacg ataacgtgcc aaaactactt gctcccgatc agtctcgatc
     8521 gtttgaagaa catggaattg ctttccccgt ccgcgtgttg tcggcggacg aggcgttcgc
     8581 gtacagggcc cagtgcgacg cgctggaggc gcagctcggc ggcaagccgc ggactgtcga
     8641 ggtgcggcag atgcacctgc atttccagtg ggcctggcgg ctggcgacgc acgagcgcgt
     8701 gctcgacgcg gccgaggacc tgctcgggcc caacctgctg atctgggcga cggagctgtt
     8761 tgccaaacac ccccgcgaca acgtcgtttc catcggctgg caccgcgacg ggccgtacat
     8821 ggggctggcc ccggagcgga cggtgaccgc ctggatcgcg ctcaccgaaa gccgccaggc
     8881 caacggctgc atgcaggtcg tccgcgaagc gagccgccag gagaaacgga accggcagcc
     8941 cgcggccagc gacgtcatcg acgaagtctc gctggcggcc ggagagatgt cgctgcacga
     9001 cgtattcgtt ctgcacggtt ccggaccgaa tctgtcggcg gaaaagcggg tcggcttcgc
     9061 gatccgcttt acgacgccgg aaggccgccc ccatgccgag cgcccgccgg cgattctggc
     9121 ccgtggcagc gaccgttacg gctatttccg actgctccag ccgccgcgcg aggacgacgc
     9181 cgaggcagcc ctcgcacaaa tgcgcggctc agcccgggcg catttggacg ctaccctgga
     9241 aaacctccag cgcgccagac gataatgttc tggcaccatg gccgagatcg aagcagccgt
     9301 ccgcgactac attctgaccg cttttctgac cggagcgcgc ggcgctgcgc ttcgtaacga
     9361 tgacgatctg cttatggtcc tggattcgct acagatgctg cgaatgctga tcgacatgga
     9421 ggctcagttt tccattcggg tagataacag cgagatgttg ccagagaacc tgggcaccat
     9481 cgagaagctc gcggcgttta tcgccagaaa gcgtcaggcc gcggggtgaa tgccgatgca
     9541 gatgtcgtcg tctgcggtgc gggcgctgcg ggccttgcgg cggcgctggc cgcggcgcgc
     9601 tcccgggcgt cggtgctgct cgtcgaacgc agtccgcaaa ttggcggcac cgtggcccac
     9661 gcgcttatcc acacgctggg cggcctctac gatgcgtacg gcaacgtgca gaacgccggc
     9721 ttgcccgcgg aactcattga ccggctgcac gaggccgatc cccgcacgcg ccgccggcgc
     9781 ctgggccgca cctgggtgct caatgtctgc ccgaacgtct atcgcgcggc cgtgacgcgc
     9841 tggattgccg agcagccggg gattaacgta atgtgccagt cgcaggcgtc gcgggttgtg
     9901 cgccgtgcga atcggatttt cgagttggaa gtgacttctc acgctcacgt gcttcgcctt
     9961 cggccgcggg ccgtgatcga cgcgaccgga accgcggaaa tggtccggtt gctcgatccg
    10021 gcgctggtca gcgacgaaga atctcgcgcc tccggcgggt ggatcttccg cctgagcggc
    10081 gtggccgccg gtgctctcga ttttcccaag ggcctggccg ccgtgcgcgc cttgcggtcg
    10141 gcagccgccg ccggcgagct gccgccgctt tcgcatcatg cgtggctcga cttcggcgtc
    10201 gatacggacg aagtgtttgt gaagctgatg gtcccgctgc agcgcgattg gcaggacgaa
    10261 gccgtggaaa ccgctaggcg agctggcgag gcacaggccg cgatcgtgca cttcctcagg
    10321 cgatttcccg attttgccca ggcggaagta acccagtcgg gcagcctggg tgtgcgcgac
    10381 ggcggacgga ttcgcgggcg ctacacgctc accgccgagg acgtgcgccg ggggcgcaaa
    10441 ttcgccgagg ccgcgggccg ctgctcgtgg ccgatcgaat actgggacgc agagcgcggg
    10501 gcgagcgtcg aatatctgac ggaaggaacg gagtacgaca ttccccaggc tgccctccag
    10561 gtctggggcg tcgataatct ttgggcctgt ggcaagtgcc tctcggccga ccgctgggcc
    10621 catgcctcgg cacgggtcgt cggcgcttgc tgggcgatgg gtcaggcggc gggaatcgcc
    10681 gcggccgcct gctcgccgca cccagggcag gaactttcga atcagcatga gcaaccttta
    10741 ccagcgattt cgtgagacgg cggcgcggcg gccggagcat ctagctattg tcggcccgcg
    10801 gggcgcgcgc tggtcgagcg ccgatttgct ggccgcgatg gacgccgccg gcgacgagct
    10861 gcatgccgcc ggcgtgcggc cgggagcttg cgtcggcctg cacgcgacca gctcggccca
    10921 gtacatcatc gacacgtacg cgatttggcg ctgcggcggc tgcgtcgtgc cgataccgac
    10981 cgagctggcg atcggcgaaa aggccgagat ttgccgcgcg atcgcgctcg actatgtgct
    11041 gtcgcccccg gccagcgccg agtttctcga cgaatctcgc cgaggaaaat cctggcagct
    11101 gggcgagcga gcgctggtcg agtggcggcc cgcccgcgag catccgtccg gctttcgcga
    11161 actggacgca gggttcattc gcttcacctc gggcacgacc ggctcgtcca agggcgtcgt
    11221 cctctcgcac accacgatcg gcgagcggat cgccggagcc aacgaggcgc tgcggctgtc
    11281 tcctgcggac cgcgtgctgt gggtgctgtc gatgtcgtat cacttcacag tttcgatcgt
    11341 cgcctattta acctatggcg cgacgatcgt cctgccggcg aaccacttgg cgcccgccat
    11401 cctgtcagcg atccgcgagc accgggcgac gatcctctac gcctcgccgc tgcactatgc
    11461 cctgctggcc gattatctcg aggccgcgcc gctcgcgagc ctgcggctgg cgatttccac
    11521 gaccagcccg ctcgacgagc gcatcgcgcg ggcctttgcc cagaaattcg gccggccggt
    11581 ctcgcaggcc ctgggtgtga tcgaagccgg cctgccctgt attcacactg atgccacggc
    11641 caatcgctgg gccagcgtcg gccgcgtgct gccgccctat cggctcaagc tggcggacac
    11701 cggcctaagc gacggtctcc gcgaggtgct gctgggcggg ccagggttcc tcgacgccta
    11761 ttacgacccc tggcagccgc ggagcgcgat catgccggac ggctggttcc gtacgggcga
    11821 cgtcggccgc gtcgatgacg acggctactt gttcctgacg ggccgctcga aagacgtgat
    11881 cagcgtcatg gggatgaaat tctttccgca agaggtcgag cgggttctcg tgatccatcc
    11941 gcaggtgcgc ggcgcgagtg tatttgccgt gaaggacccg cggtggggcg agcgggcgat
    12001 ggcccgcgtg gtcgccgcgg ctgatgcgcc gggcgaggca ctcgccgagg agctgcggag
    12061 gcattgccag cagtcgctag ccgcctataa ggttcccgag cggattgagt tcgtgcccgc
    12121 tttgccgcga acggccagtg gcaaaatcct gcatcgctcg acgtagaagg acgcccgctt
    12181 gaagccgaac cccgatcggc aattgctcaa tccgctcgac gtcgcactgc ggtgcatcga
    12241 ccacgccatc cgcggcatgg gttttcccgg gttcgagacg cagatgctcg tcgctctcgc
    12301 cgagcgcgtc gatgtgccgc ggctgcgaaa tgctctgact cgcttcggcc ggcgtcatcc
    12361 ggtcgtcacg gcccggctcg tcgaagacga gggccgacac ccgtcgttct ggcaattccg
    12421 gcccggcgca attccgcagc tgcccgagat cgagctgtct tccgccgaac cgagtgcggt
    12481 tctggccgcg gccgccgagc tgctttcgac gccgcaggac ccagccgccg cggatcccct
    12541 gcgatttcac ctgctccatc ggcccggcgg cggcgacgtc ctcgtaattc agtacaacca
    12601 cacgctgatc gacaacagcg cgacggccct gatcgtccgg gaaatcgacg ggctgtcgca
    12661 gacgggcggc acggccgaag aggaccaacc acaatttgag ccgcggttct tgatcggcaa
    12721 ctatctccgc cacgtgccgc atgccgaccg ccgggcagcg gcccaggcgg cgattgagct
    12781 gcaggcccac atcattcgca gccgcggggc cctgctcgga aggggcgaga aaaacgtaac
    12841 gcgacccccg cggctgaaag tcgccgcccg tgtcgccgag ccggagctga cgcgagcgat
    12901 ccgcggccgc gccgcggagc tgtgcggcct gccgagcctg tcgatggcca tgctgggcag
    12961 cgcctttcgg ggaattcgcg cattgggctc acccgagcac aacaccggcc ggcactataa
    13021 cgccggcatt ggcctggacc tcgacctgcg cgaggaaagc ggcgctctcc tgcagaacct
    13081 gctctcgctg gtggccatct atgcgcgggg cgacgagctg gccgatcgcg acggacttgt
    13141 ccgcagcctg agcctgcaga tgcgcgaccg actcgtgcgg cggctcgatc tgggcatcgt
    13201 ccgcctcgtc cacgtctttc accgccgacc gcgctacgtg agctgggtga tggggcatct
    13261 gctccgctgg agctgctcgg tgtggtatgc ctacttcggg gcgctcgact cggtcggtcc
    13321 gcggttttgc ggcgtacccg tcgagcgcgt gcagtacatc ggcccgacct ggtcgcccaa
    13381 ggggatcgcg ctgctcgtcg atcagtttgc cgggcggctg tacctgcagc tcactttcga
    13441 tccggagctt gttgccgagc cgctggccaa cacgtacctg gactggctga tgggcgacct
    13501 ggaggagttc gcggcggcta gtccgccacg atcgctttga ggacggcggc cagccggtag
    13561 ctcgcctgca cgacgcgccg ctcggcattg cggccgccca tccgcaggta atcctcggag
    13621 agcgtcagcg gctcgggcgg atcagcgctg gcttcgacct tccgcagcgc gatcatcaat
    13681 tccgcatcgt acacgaccgt cacggccacc aggtggctct catcgcgcca actctttgcg
    13741 tccaagtctg cggccgcctc ctggccaagc ttcatccatt tggcatcggc ggacatggcg
    13801 atcgcccggt tccgcaccgc acgaaactcg gcgtcctgcc caagaaagcc atcccacagg
    13861 gcgtgcagat tgaacgactg cacgagcttg atcttgttgg ccccctggtc gccttccggg
    13921 aatagccggt tggagtacag ctgtgtcgaa tggcacggct gatgcaagtc gcctaccgcg
    13981 tgaaagagcc aggccagcat cagtgcccgc gtttcgggcg cggcttgttt gtcggcggcc
    14041 agccggcggg ccaggcggat cgtctgcgtg acgttcatct cctggagctc gggcgtggca
    14101 gtcgggggcg gatcgagggc gacgttttcc ttgatctggc cgtccaagtc ggcccgcgtg
    14161 gtatcgttca agaagtgcgg cagatcgacg taatgccagg tcggccggtg aaaggccgtc
    14221 cgttctggcg ggccgccgcg aacgatgtcg gcccagatgc ccgcctgctg aaacagccat
    14281 tcgttctgca tcgcctcatc gccagctttg acttccggcg gcatttcgtc ggcaaagtcg
    14341 ggcgtgaacc gcggatgccg ttggaggatc gccaccacgc ggccctggtc ggccgccgac
    14401 agctgccgga aggccagcga cgaaattagc ttgtgccccg tggcgttcca ggcgccggcg
    14461 ggcgaaacgg ctacgaggca tacgagcagc gaaacgatgg gggcgcgcat gttcgttttc
    14521 ggcgggtagc tgagaatcgt cgcccaatcc tacttcgcaa tagccgcggg cgacatgccg
    14581 cgcaatttgt gccgcaatgg attacaactg gaaggcggct gaatttgcgt gttgaacagg
    14641 acgagatggc atgaaccgag gcttgcttgc ggcgctcgtc ttgcttgcgg ctggcgcagt
    14701 ctgcgccgaa gagccgcggg cggcgctcga ctatttcgag cgcgaggtcc gtccgatcct
    14761 ggtcgatcac tgccagaagt gtcacggcgc gggcaagcaa gagtcggacc tgcggctgga
    14821 ctcgcgcgag gccctgctca agggcggcgt ttccggtccg gcaatcgtcc ccggacagcc
    14881 cggcaagagc acgcttatcg cggcgattcg ccacgagggc gacatcaaga tgccgcccaa
    14941 gtcgaagctg accgaggctc aaatcgccgc cgtcacgcac tgggttgcct ctggcgcggt
    15001 ttggcccagt gcgatcgccg tcgccaagtc gggcgactcg cggctcacgc attgggcgtt
    15061 ccagccggtg cgcgatccgc cggtgcccgt ggtcgcgggc gatcagtcgg cgaacccgat
    15121 cgatgccttt gtgctcgaga agttggccgc ggcgggcctc tcggcgtcgc cgccggccga
    15181 tcgccggacg ctgattcgcc gcatgacggt cgatttgacc ggcctgctgc caacagcaga
    15241 ggaagtggaa gcctttgagc gcgacagcga ccccaaggcc gccgaggcgc tgctcgaccg
    15301 tctgctcgct tcgccccact atggcgagca gcaggctcgg cactggctcg acgtggcccg
    15361 ctattccgac acgaagggct acgtctatgg ccgcgagcag cggttctggc tgcatgcctg
    15421 ggtctatcgc gactgggtcg tgcgggcgtt caacgacgac ctgccgtacg atcgcttcct
    15481 gctgctgcaa attgccgccg accaggccgc gccggacgac aaggcggcgc tgtccgcgat
    15541 gggctttctc acgctcggcc gccgattcct cggcgtgcag cgggacatca tcgacgaccg
    15601 gatcgacgtg gtgacgcgcg gcacgatggg cctgaccgtc ggctgcgccc gctgccacga
    15661 ccacaaatac gacccgatcc cgacccgcga ctactactcg ctgtatggcg tcttccagag
    15721 ctccgccgag cggagcgtga cgatcgccga gcctgcccag ccggacgagg cgtatcagaa
    15781 gttcgcggag gaattggaga agcgtcgcaa gaacctcgcc gacggcctgg cggcgcggcg
    15841 gctggaaacg gccgaccaca accgctcgcg taccggcgat tatctggcgg cgcagctcga
    15901 attacacaac tacccgcccg agggctttga ccagattttc tccaagacgg atttgctgcc
    15961 gaccttcgtg catcgctggg aggcgtatct ggcggacgcc aagaagcgca gcgagccgat
    16021 ctttgtcccg tggcacgcct actccgagat tccggcccgc gactttgcca ccaaggccgc
    16081 cgccgttacg gagcaattga actcacggcc ggacggcgcg atcaatccgc tggtcgcggg
    16141 caagttcgcg gccgcgccgg cttcgatggc cgaagttgcg gcccgatacg gcgaactatt
    16201 cgccgagatc gaagcccagt ggaaaaagtc cgcccccgcg ccgctggccg atccagcggc
    16261 cgaagccctg cggcaggtgc tctacagcag ggcgggcccg tgcgaagtgc ccgacgagcc
    16321 gatcgtcaat atcgagtact tcgtcgattc tggttcctgc gatgcgctct ggaaactcca
    16381 gaacgaggtc gataactgga ttctgcaggg accgccgtca atgcggatgg cggtcattct
    16441 caccgaccgc aatccgccga tcgagccgca cgtgtttagg cgcggcaacc ccgtcaacct
    16501 gggcgaggag attccgcggc agttcgtcga agtgctcgcc gggccagacc ggcggccgtt
    16561 tgccagcggc agcggccggc tggaactggc ccaggcgatc atcgacccca agaacccgct
    16621 caccgcccgc gtcatcgtca accgcatctg ggcgcaccac tttggctctg gcctcgtccg
    16681 cacgcccagc gacttcggca cgcgggccga tccgcccagc catcccaagc tgctcgattg
    16741 gctggctagc cgattcatcg aagacggctg gtcgattacg aagctgcacc ggcggatcat
    16801 gacgagccgg acgtatcagc aggcctccag cgcggagcgc ggagcgggga gcgcggagca
    16861 gggagttccg agttccgtcg atcccgaaaa ccgcctgctc tggcggcaga acccgcggcg
    16921 gctgtcgttc gaggaaatgc gcgatgcgct cttggccgcc ggcggccagc tcgaccgcac
    16981 gctcggcggc cgggcgggcg atctgtttgc ccgcacgttc gtccgccgct cgctgtatgg
    17041 cacgatcgat cggcagttcc tgcccagcac gctacggatg ttcgatttcg ccaatccgga
    17101 cctgcacatc ccactccgca gcgaaacgac cgtgccgcag caggccctgt tcttcctcaa
    17161 ccatccgttg ctgatcggtt atgcgcagac cgtggcgaag gcgacggcca gcgccgccac
    17221 ggacgaagag cgcgtgcggc aactctttcg ccgcgcctac cagcgcgagg cgacggctga
    17281 cgaaatccac tccgccctgg cgctgatatc ggcggctgcg gaaggcccgc aaacggcgat
    17341 ccctgtcacg gcgaccgcct ggcaatacgg ctacggggct ttcgacgaaa agatgcagcg
    17401 cgtcaccggc ttcaaaaagc tgccgcactt caccgggacg gcctggcagg gcggctcggg
    17461 ctatcccgac ggcaagctcg gctggctgca gctcacggcc gagggcgggc accccggcaa
    17521 cgacctggcc catgccgtcg tccgccgctg gaccgcgccc cgcgacatgc aggtccgcgt
    17581 cacttcgacg ctcatccacg agccgaagga aggcaagggg gtccgcggct tcctggtcag
    17641 cagccgcggc ggcctcatca agcaagttgc ggttcacaat ggccgcgaag agctcaacgc
    17701 cgaccagctc gacctgcagg ccggcgacac gctggacttc gtcgccgaca tgggtgacct
    17761 gctgagccac aaccaattcc tctggaaggc ccagatcacc gccgccggct cgggcggcct
    17821 caccttcgac tcccagcgcg acttcggcga ccagcccacg cccaagctca ccgcttggga
    17881 acaactggcc caggtgctgc tgtcggcgaa cgagtttgtg tttgtggatt agccccagcc
    17941 gcgccagtcg gacaaaaggc agtcgctccg cggttgaacg aacgaacttg gtcaaatcga
    18001 gccgtaggcg ggtagaataa ctctgatgcg ttaccgcctc cgtacgctga tgattgcctt
    18061 ggggatgggg ccgccggtaa tcgcgggggt ttggtgggga gtggcttgga ctgtcgcgtg
    18121 gcttgtcagc agacctggac cacacctctt cgtcctggtg tccgcggtgg ccgtcctgta
    18181 tctggcggca gccgcggtgg ccgtggccat gaaagagctg cgcggcgcta aacgattcga
    18241 ttggctgatg attgcagcgg gaatcggcgc agcgctagcc gggcttggcg tcattgcctt
    18301 gttgaaataa tggccggaac ttggtatata tacaagggtt tataattcga tacagcaggt
    18361 caagatgcat gcttaagttg cataaactgc atagccgcat gcgagaaacg tcatcgcaag
    18421 tcgttgcctt ataaagactt gcaatccacg gccagcgcgg gtattttcgc gaatatgcag
    18481 aataagccga tgcagccagc cgcctttgtg agccgccgcg acatgttgcg ccgggctggg
    18541 acgggcctcg ggatgctggg gctggcgtcg ttgatggcgg acgatggcct gctagcggcg
    18601 gaggcggggc cgctgacgcc gaagcatccg cactttgcgc cgcgggccaa acgagtggtg
    18661 cacttcttct gcaacggcgg cccgtcgcac gtcgatacgt ttgaccccaa gccggcgctg
    18721 gccaggcacg cggggcagcc gctaccgcag acgctgcgaa ccgagcggaa aacgggggcg
    18781 gcgttccctt cgccgttcaa gttccagaag tacggcgaaa gcggcctgga aatcagcgac
    18841 atcttcgccc gcacggccga gcacgccgac agcctggcgg ttatt
//
LOCUS       DASRJB010000392        10345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3775156, whole genome shotgun sequence.
ACCESSION   DASRJB010000392 DASRJB010000000
VERSION     DASRJB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3775156"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFB80_10405"
     CDS             <1..371
                     /locus_tag="VFB80_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34223.1"
                     /translation="LSPTAPGDYAIELTAWQGLQKLGTAKAEFLVFDRDVELSNPAAD
                     PDLMASLAAWTKQEGGRVVGAEELPKLLGELAAKPPEYEVRQKRWKLAGTAGDAWLML
                     VLMTAVLTGEWFLRKRWGLV"
     gene            complement(553..1800)
                     /locus_tag="VFB80_10410"
     CDS             complement(553..1800)
                     /locus_tag="VFB80_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN34224.1"
                     /translation="MNLDFDVIVIGAGAAGLVAAAEAAERGRRTLLLEKNRRAGVKIL
                     MSGGTRCNLTHATDKWGIIRAYGEQGQFLHSALAALSPADLVKLIEDEGVPTKVEETG
                     KIFPVSNRATDVLEALLRRLSRSGAELALSEPVQRIESLAAGGFRTASSSRELTAEKL
                     IVTVGGQSYPGSGTTGDGYAWAAACGHTIVPPRPALVPLTTSEVWVRELSGVTIPDVA
                     LKLFLRASPTADKPLDNRRGSLLFTHFGLSGPVVLDISRGVTALADPAAADVVCDFQP
                     DLPPPQCDELLRQATAADGKRPLAAFLAERLPRRLADALVVLAGLKAELRGAEFSKAA
                     RGQLVRAVKSTAIPITGTRGFAKAEVTAGGVALDEIDSRSMQSKLVKGLYFAGEILDL
                     DGPIGGYNFQAAFSTGVLAGRSA"
     gene            complement(1868..2068)
                     /locus_tag="VFB80_10415"
     CDS             complement(1868..2068)
                     /locus_tag="VFB80_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34225.1"
                     /translation="MLFAAALLAKQIWYIVPLILSVSLVYGATRHELPRPILYHAWHT
                     GVWMAIFMGGIFVVLWLVSWWL"
     gene            complement(2094..2648)
                     /locus_tag="VFB80_10420"
     CDS             complement(2094..2648)
                     /locus_tag="VFB80_10420"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HZN34226.1"
                     /translation="MSTSAPPPAEPTLEIDLRDPHVAALFAWLWPGAGHLYQRRYLKA
                     VLFAVCILATYFFGLALGEGKVVYAAWNSADKRWQYPLQLGVGLPAAPALVQSLIVRR
                     GGDPILGGLMRPPRPGELSQWHRDLNLRFELGTLYTMIAGLLNILAIWDAYGGPVIGE
                     PGKERGPPDGDKSKDKDKDKPKTP"
     gene            2894..3715
                     /locus_tag="VFB80_10425"
     CDS             2894..3715
                     /locus_tag="VFB80_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34227.1"
                     /translation="MTRWLWQAALVATLAAPGCGGGPLPAGPAAEQPQEILRRELAQF
                     PKIGKYEPPLDSYRVQIAPPEGWRATQGLNFLIGFKPQGQGELPRITVIAEDPPAGYP
                     ADLTEVEAPGLALLLDQGLRKAVAENQKSVEEFSLPIVLGENVFIRHVRRAQTGGAKC
                     IVQSLATIKNSRMYTVELIAAIEPPQSAEYEASLLKYRDYAYAVAGHMRFAPPGKTFD
                     PSAGPKPKPPLTEAKKAAPAPEAATPKTAPPKSAPAAPAGEKPNKSEKTTEKKSA"
     gene            3733..3933
                     /locus_tag="VFB80_10430"
     CDS             3733..3933
                     /locus_tag="VFB80_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34228.1"
                     /translation="MDEQLKQQLMPAIVVFNLTVIAWVLYRFFTAQRGMFFQDFVIQA
                     LIGAAIGLATGGVTLLVTMRKK"
     gene            complement(3914..4312)
                     /locus_tag="VFB80_10435"
     CDS             complement(3914..4312)
                     /locus_tag="VFB80_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34229.1"
                     /translation="MPRVLDVGNCDLDHGAIRRMLAASFRAEVVRAHDAAGALALVRA
                     QPFDLVLVNRQLDADHSDGLPIIEAIKADPKLRQVPCMLITNYAQHQAAAERAGALPG
                     FGKKEIQSDQTRARLAAILAPPSAATSSAS"
     gene            complement(4505..6676)
                     /locus_tag="VFB80_10440"
     CDS             complement(4505..6676)
                     /locus_tag="VFB80_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34230.1"
                     /translation="MRHLQALAAIFAALVCGTIAVTLLAGCQPAVPPPSKTSASGTTS
                     STTSGRLPSTPAAEVPDDKPTPEKPEDKPADKSKEATPGAGDTAAKSKLANPALKAGD
                     WNQWGGNSLRNNTPVAEGIVTEWAPGAFDRKTGAWKPESAKNIRWVTALGSQTYGNTV
                     VAAGKVFVGTNNSSGYLKRYPADIDLGVLVCLNEADGKLLWQHNSEKLPTGRVHDWPL
                     MGICCSPLVEGDRLWFVTSRGEVRCLDVDGFHDGTDDGRPESAEPARLFDVNRADDPA
                     EDKVGGYIAALDKGEMPADLRPKFEAAGMPLPEGDVQVTADPKAKAPAKKWTFKADVN
                     GSSRDFILTVAGPKLSAFKIITPADKDEADVIWSFDMMKELGTSQHNMCSCSVTSLGD
                     ILFVNSSNGVDEAHIVIPAPEAPSFIAMDKNTGKVFWTDNAPGKNILHGQWSSPTVAV
                     LGGVPQVIFGGGDGWIYSFKADKGKEGKPERLWRFDINPKDSVLELGGRGTRNDIIST
                     PVVYDDKVYFCTGQDPEHGEGAGIVWCIDPTKRGDISEKLAVNRAAPKKPIPVKREQA
                     VVEADGDLAIDNPNSGVVWKYAVNDANGDKKIDFEEQFHRSICTAAIKNDLLYLPDFS
                     GLFHCLDAKTGKVHWTYDMLAAAWGSPLIVGDKVYVGDEDGDILVFNLTKEKHDPVFE
                     VNMGSSVYSTPIVANGVLYIAGKDHIFAIAPPKNEAAKTGE"
     gene            complement(6753..9005)
                     /locus_tag="VFB80_10445"
     CDS             complement(6753..9005)
                     /locus_tag="VFB80_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34231.1"
                     /translation="MKRPLPSLALASRLLVVWTSIFLGASALAQEAKVDPLDWPYWRG
                     PEYNGISRETGLPDKINPDGGEGSNLLWKKPEFGGRSTPIILRGKLYTILRADPGTPT
                     EGERVVCIDAATGELKWESRHNVWSSDVPDTRVGWSSVAGDPETGNVYALGACGLFEC
                     YDGETGKILWSIPLHEQMGLLSTYGGRTNFPVVFEDLVILGSVIIGWGDMAVPAHRLM
                     AFNKRTGEVVWFVSTRLRPPDTIYSAPVIATINGQKLLIGGASDGWIYAFQPRTGKKV
                     WEYQFSRRGINMSPTVDGDVVYVGHSEENPFGTKMGAVAAIKGDLSGDITLKAEIWKE
                     LETYGVGRSSILKVEDRLYLFDDPGKVWVVDAKTGERIGNKQTLGTMNFASPLFADGR
                     IYHCEKNGRIFILTPDEKGGIKITRDSRVTLPTGDECWASPVVSHGRLYILTTGAMYC
                     FEDKSKPHASAPGPAAAEEAPVNTDEKPAQVQVVPCELLLGPGAKQKLTVKLFNSRGQ
                     FLKESPAELTLSGPGKIDNGEFAAPEGTAHTAAIVTAKVGELTGRSRIRVVPPLPWKF
                     DFEGLTDAPVTWVGARYRHVVRQVDGSNTMVKITTIPLGTRSRLSMGPPNLHDYTVQG
                     DFKVAANPGGKLPDVGAIAQGYTLEVSGENKWIKLVSWLPHPKRHFKELPFDLKPDSW
                     YTIKLRAENSAGKALLRGKVWERGKPEPKDWTIELVDPMPNTTGAPGLFGNATNAELF
                     LDNVSVTPNS"
     gene            complement(9196..9558)
                     /locus_tag="VFB80_10450"
     CDS             complement(9196..9558)
                     /locus_tag="VFB80_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN34232.1"
                     /translation="MVVTEKPPYELVDFAALPGIPCPCGTARRGLADVEDYPGTIHVT
                     EIALDNALHYHKRLTETYYFLECEPGAKMQLNDELLDVRPGTCIMIRPLTRHRAIGRM
                     KVLIVVFPKFDPADEWFD"
     gene            complement(9630..10127)
                     /locus_tag="VFB80_10455"
     CDS             complement(9630..10127)
                     /locus_tag="VFB80_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN34233.1"
                     /translation="MTADFAIKQIEFARSYLLSILAEIDEQDWFTMPAGCPTHVAWQI
                     GHLAMAEYGLCLYRMRGRQPVDLELMTSSFRKQFSRGTVPEADAAKYPAAAEIRATLD
                     RVHAQVLKEAPSFTAEQLKEAVEMPYAVEATKLGGLLFCSHHEMLHAGQLGLLRRLLG
                     KTPIR"
     gene            complement(10146..>10345)
                     /locus_tag="VFB80_10460"
     CDS             complement(10146..>10345)
                     /locus_tag="VFB80_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34234.1"
                     /translation="RAHPLIVVAGMLLFVALATGLLCLLFTPLAYRVRKTAPPRSIAV
                     AAVLIGLSPIITFAVLAVLPH"
BASE COUNT         1735 a         3373 c         3375 g         1862 t
ORIGIN      
        1 cgctttcgcc gaccgcgccc ggcgattacg cgatcgagct gaccgcctgg caaggtttgc
       61 agaagctggg cacggcgaag gccgagttcc tggtctttga ccgcgacgtg gagttgagca
      121 atccggcggc cgacccggac ctgatggcct cgctcgccgc ctggacgaag caggaagggg
      181 gccgggtggt cggcgccgag gagttgccga agctgctggg cgaattggcc gccaagccgc
      241 cggaatatga agtccgccag aagcggtgga agctcgccgg caccgcgggc gacgcctggc
      301 tgatgctcgt cctgatgacc gccgtcctga ccggcgagtg gtttctgcgc aagcgctggg
      361 gactggtgta gtcaaagccg cgaccagtgg tcgcggtctc cgctcggtcc caagctccgc
      421 tcggtcccaa ctccgctcgg gaccgcacct ctgcaagctc ggcttgccaa atgtgaagca
      481 gagcttccgt cctggcgtgc ccaagcggag cttgggcaca agaaaaccgc gaccactggt
      541 cgcggctttg agttacgcgc tccgccccgc cagcactccc gtgctgaagg cggcctggaa
      601 gttgtatccg ccgatcgggc cgtcgagatc gagtatctcg ccggcgaagt agagcccctt
      661 aacgagcttg ctctgcatcg agcggctgtc gatctcatcc agcgcgacgc cgccggcggt
      721 gacttctgcc ttggcaaagc cgcgcgtgcc ggtgatcgga atcgccgtgc tcttcacggc
      781 ccgcacgagc tgcccgcggg cggctttcga gaattccgcc ccgcgcagtt ccgccttcag
      841 gccggccagc acgacaagcg catcggctaa tcgccgcggc agtcgctcgg cgagaaacgc
      901 agcgagcggc cgcttgccgt cggcggccgt tgcctggcgg agcagctcgt cgcactgcgg
      961 cggcggaaga tcgggctgga agtcgcacac cacgtcggcc gccgcggggt cggccagcgc
     1021 ggtcacgccg cggctgatgt ccagcacgac gggccccgac aagccgaagt gcgtgaagag
     1081 cagcgagccg cggcggttat cgagcggctt gtccgccgtt ggactggcgc ggagaaagag
     1141 cttcagagcg acatcgggaa tggtgacgcc gctcagctcg cggacccaca cctccgaagt
     1201 cgtcagcggc accagcgccg ggcggggcgg cacgatggta tggccgcagg ccgcggccca
     1261 ggcgtagccg tcgcccgtcg tgccgctgcc aggatacgac tggccgccga cggtgacgat
     1321 caacttttcg gcagtcagct cgcggctgct gctggcggtg cggaacccgc cggcagcgag
     1381 cgactcgatt cgctgcaccg gctcggacag cgccagctcc gcgccgctgc gcgacaagcg
     1441 gcggagcagg gcctcgagca cgtcggtggc ccggttgctg acggggaaga tcttgcccgt
     1501 ttcttcgacc ttggtcggaa cgccttcgtc ttcgatcagc ttgaccaggt cggccggcga
     1561 gagcgcggcc agggcggagt ggaggaactg cccctgctcg ccgtaggccc gaatgatgcc
     1621 ccatttgtcg gtggcgtgcg tgaggttgca gcgcgtgccg ccggacatca ggatcttcac
     1681 gcccgcgcgg cgattcttct ccaaaagcag cgtccgcctg ccgcgctcgg cggcctcggc
     1741 agccgcgacc aggccggcag cgcctgcgcc gatgacgatg acgtcgaagt ccaagttcaa
     1801 agttcaaagt tcaaggttca aggtcaagat cgcaactttg aactttggac tttgaacctg
     1861 gaactgtcta gagccaccag gagacgagcc acaggacgac gaagatgccg cccatgaaga
     1921 ttgccatcca gacgcccgtg tgccaggcgt gatagaggat cggccgcggc agctcgtggc
     1981 gcgtggcgcc atagaccagg ctgaccgaga ggatcagcgg gacgatgtac cagatttgct
     2041 tggcgagcag cgcggcggcg aaaagcatgg ggatattcac ggcggattgc gcctcagggc
     2101 gtcttgggtt tgtctttgtc tttatctttc gacttgtcgc catccggagg cccgcgctcc
     2161 ttgcccggct cgccgatgac gggaccgccg taggcgtccc agatcgcaag gatattgagc
     2221 agcccggcga tcatcgtgta gagcgtgccc agctcgaagc ggagattgag atcccggtgc
     2281 cactgcgaca gctcgccggg acgcggcggc cgcatcagcc ctcccaggat cggatcaccg
     2341 ccccggcgga cgatcaggct ttgcaccagg gccggcgcgg ccggcaggcc gacgccaagc
     2401 tggagcggat actgccagcg cttgtcggcc gagttccagg ccgcatacac gaccttgccc
     2461 tcaccgaggg ccaggccgaa aaagtaggtc gccaggatgc agaccgcgaa cagcactgcc
     2521 ttcaggtacc gccgctgata gaggtgcccc gcgcccggcc acagccaggc gaacagcgcc
     2581 gccacgtgag gatcgcgaag gtcgatttcc agggtcggct cggccggcgg cggggcggaa
     2641 gtggacatga tcgaggcggg agcaaggagc aaggcctggt ggaatctgct tcaaaccgac
     2701 attctatcga tccccgcggc ggggaacaga ccaaccggtt gccgcggctc gcgcagcaag
     2761 ggcttgttcg ttcgccaggg atcgatcttg aaccgcgtcc gcgcccgact ggcgaggaat
     2821 gccccgcagg ttatcatgca cggcacgcac gcggccgctg tggccgcaac gggcggccgc
     2881 aacgggaggg acagtgacca ggtggctgtg gcaagcggcg ctggtggcaa ccctggcagc
     2941 gccgggctgc ggcggcggcc cattacccgc cgggccggcg gccgaacagc cgcaggaaat
     3001 cctccgccgc gagctggccc aattcccgaa aatcggcaag tacgagccgc cgctggacag
     3061 ctaccgggtg cagatcgcgc cgccggaagg ctggcgagcg acccaggggc tcaattttct
     3121 gatcggattc aagccccagg ggcagggcga gctgccgcga atcacagtaa tcgccgaaga
     3181 tccgccggcc ggttatcccg ccgatttgac ggaggtggag gctcccggcc tggccttgct
     3241 gctcgaccag gggctgcgca aggcggttgc cgagaatcag aagtccgtcg aggagttcag
     3301 tctgcccatc gtattgggag agaacgtctt cattcgccac gtccgccgcg cgcagaccgg
     3361 cggcgcgaag tgcatcgtgc agtcgctggc gacgatcaag aactcgcgga tgtacacggt
     3421 cgagctgatc gccgcgatcg aaccgccgca gtccgccgag tacgaagcct cgctgctgaa
     3481 gtaccgcgac tatgcctacg ccgtggcggg gcacatgcgg ttcgctccgc cgggcaagac
     3541 gttcgatccc tcggccggac cgaagccgaa gccgccgctc accgaggcca agaaggccgc
     3601 gcccgctccg gaagccgcga cgcccaagac cgcgccgcca aagtccgcac cagccgctcc
     3661 cgccggcgag aagccgaaca agtccgagaa gaccaccgag aagaaatccg cctaacccag
     3721 ccgcatcact ccatggacga acaactcaag cagcagctca tgccggcgat cgtggtcttt
     3781 aacctgaccg tcattgcctg ggtcctgtac cgcttcttca cggcccagcg cggcatgttc
     3841 tttcaagact ttgtgatcca ggcgctgatc ggggcggcga tcggcctggc gaccggcggc
     3901 gtcacgctgc tggtcacgat gcggaagaag tagcggcgga tggcggcgcg agaatcgccg
     3961 cgagcctcgc ccgcgtctgg tccgactgga tttccttctt gccgaagccg ggcagagcac
     4021 cggcgcgctc agccgccgcc tggtgctggg cgtagttcgt gatcagcatg caggggacct
     4081 gccgcaactt tggatcggcc ttgatcgcct cgatgatcgg caggccgtcg ctgtggtcgg
     4141 cgtcgagctg gcggttgacg agcaccaggt cgaacggctg cgcgcggacc agcgccagcg
     4201 cgccagcggc gtcgtgggcc cgcacaacct cggcgcgaaa gctcgccgcc agcatgcggc
     4261 gaatcgcccc atggtccagg tcgcagttgc cgacgtcgag tacgcgaggc ataggcaaga
     4321 aatcccaaga accaaagaac caaaactcca aagttagttc tttcctcgtg cccaagctct
     4381 gcttgggcac gcagatttcg aagctccact tcgcgtgggc gggaagcaga gcttctcaag
     4441 cgtgggttcc caagcggagc ttgggaacca gtttggttct tcggttcttt ggttctttga
     4501 ttttctactc cccagtcttc gccgcttcgt tcttgggtgg ggcgatggcg aagatgtggt
     4561 ccttcccggc aatatagagc acgccattgg cgacgatggg cgtgctgtag accgagctgc
     4621 ccatgttgac ctcgaacacc gggtcgtgct tctccttggt caggttgaag accaggatgt
     4681 cgccgtcctc gtcgccgaca tagaccttgt cgccgacgat caacggcgag ccccaggccg
     4741 cggccagcat gtcgtaggtc cagtggacct tgccggtctt ggcatcgagg cagtggaaca
     4801 ggccgctgaa gtcgggcagg tacaacaggt cgttcttgat cgccgccgtg caaatcgagc
     4861 ggtggaactg ctcctcgaag tcgatcttct tgtcgccgtt ggcgtcgttg accgcgtact
     4921 tccagaccac gccggaattc ggattgtcga tcgccaggtc gccgtcggcc tcgaccaccg
     4981 cctgctcccg cttcaccggg atcggcttct tcggagcggc ccggttgacc gccagcttct
     5041 cgctgatgtc gccgcgcttg gtcgggtcga tgcaccagac gatgcccgcc ccttcgccgt
     5101 gctccgggtc ctggcccgtg cagaaataga ccttgtcgtc gtagacgacg ggcgtgctga
     5161 tgatgtcgtt ccgcgtgccg cggccgccca actccagcac cgagtccttg gggttgatgt
     5221 cgaacctcca gagccgctcg ggcttgcctt ccttgccctt gtcggccttg aaactgtaga
     5281 tccagccgtc gccgccgccg aagatgacct gcggcacgcc accgagcacg gcgacggtcg
     5341 ggctgctcca ctggccatgg agaatgttct tgccgggagc gttgtcggtc cagaaaacct
     5401 tgcccgtgtt cttatccatc gcgatgaagc tcggcgcctc gggggccgga atcacgatgt
     5461 gcgcctcatc cacgccgttg ctcgagttga cgaacaggat gtcgcccagc gaggtcaccg
     5521 agcagctgca catgttgtgc tgcgacgttc ccagctcctt catcatgtcg aacgaccaga
     5581 tcacgtccgc ctcgtccttg tccgcgggcg tgatgatttt gaaggccgac agcttcggcc
     5641 cggcaaccgt caggatgaag tcgcggctgc tgccgttgac gtcggccttg aaggtccatt
     5701 tcttggcggg ggctttggct ttcggatcgg cggtcacctg cacgtcgccc tcgggcagcg
     5761 gcatgcccgc ggcctcgaac ttgggccgca aatcggccgg catttcgccc ttgtcgagcg
     5821 cggcgatgta gccgccgact ttgtcttccg ccggatcgtc ggcgcggttc acgtcgaaca
     5881 gccgggcggg ctctgccgac tcgggccggc cgtcgtcggt gccgtcgtgg aagccgtcca
     5941 cgtcgaggca gcgaacttcg ccgcggctgg tcacgaacca caggcggtcc ccttcgacca
     6001 gcggcgagca gcagatgccc atcagcggcc agtcgtggac gcggccggtc ggcagctttt
     6061 cgctgttgtg ctgccagagc agcttgccgt cggcttcgtt caggcagacg agcacgccca
     6121 agtcgatgtc ggccggataa cgcttgagat acccggagct gttgttcgtg cccacgaaga
     6181 ccttgcccgc ggcgacgacc gtgttgccgt aggtctggct gcccagggcc gtgacccagc
     6241 ggatgttttt ggcggactct ggcttccagg cgccggtctt gcggtcgaaa gcccccggcg
     6301 cccactcggt cacgatgccc tcggcgacgg gagtgttgtt gcggagcgaa ttgccgcccc
     6361 attggttcca atcgccggcc ttgagcgcgg gatttgccag cttgctcttg gccgcggtgt
     6421 cacccgcccc cggcgtagcc tccttggatt tgtcggccgg cttgtcctcg ggcttctccg
     6481 gcgtcggttt gtcgtcgggc acttcggccg ccggtgtgct cggcagtctg ccggatgtcg
     6541 tgctcgacgt cgttcccgag gcggatgttt tcgacggcgg cggcacggcc ggctggcagc
     6601 cggcgagcag cgtcacggca atcgtcccgc agaccagggc ggcgaagatc gccgcgaggg
     6661 cttgtagatg tcgcatcgat cagttctccc gcggcggaga aaagccgccg ctactgaatg
     6721 tagccgaatt cgccggaatt cggagttcgg gtttatgagt tgggggtgac actgacgttg
     6781 tccaggaaca gctcggcgtt ggtggcgttg ccgaacaggc cgggtgcgcc ggtggtgttg
     6841 ggcatcggat cgactagctc gatcgtccag tccttgggct ccggcttgcc gcgctcccag
     6901 accttgccgc gcaagagcgc cttgccggcc gagttctcgg cgcggagctt gatcgtgtac
     6961 cacgaatccg gcttcaggtc gaagggcagc tccttgaagt gccgcttggg gtgcggcagc
     7021 cagctcacca gcttgatcca cttgttctcg cccgagactt ccagcgtgta gccctgggcg
     7081 atcgcgccca catcgggcag cttgcccccc gggttggccg ccactttgaa atcgccctgc
     7141 accgtgtagt cgtgcaggtt cggcgggccc atcgacaacc ggctgcgggt gcccagcggg
     7201 atcgtcgtga ttttgaccat cgtgttgctg ccgtccacct gccgcacgac gtggcgatag
     7261 cgagcgccga cccaagtgac cggggcgtcg gtcagtcctt cgaagtcgaa cttccaaggc
     7321 agcggcggca caacgcgaat ccgcgagcgg ccggtcaact cgccgacttt ggcggtaacg
     7381 atcgccgccg tgtgagccgt gccttccggc gcagcaaact cgccattgtc gatctttccg
     7441 ggcccggaaa gcgtcaactc ggccggcgac tccttgagga actgcccgcg gctattaaac
     7501 agcttcacgg tcagcttctg cttagcgccc gggcccagca gcagctcgca cggcaccacc
     7561 tgcacctggg ccggtttctc gtccgtgttg accggggctt cttcggccgc cgccggccct
     7621 ggcgcgctgg cgtgcggctt cgacttgtcc tcgaagcagt acatcgcgcc ggtcgtcagg
     7681 atgtagagcc ggccatgcga aacgacgggc gacgcccagc attcgtcgcc ggtgggcagc
     7741 gtgacccgcg agtcgcgggt gatcttgatg ccgcctttct cgtcgggcgt gaggatgaaa
     7801 atccggccgt tcttttcgca gtggtagatc cggccgtcgg caaacagcgg cgaggcgaaa
     7861 ttcatcgtgc ccagcgtctg cttgttgccg atccgctcgc cggtcttggc atcgacgacc
     7921 cagaccttgc cgggatcgtc gaacaagtac aagcggtcct cgactttcag aatcgagctc
     7981 cggccgactc cgtacgtctc caactccttc cagatctcgg ctttcagcgt gatgtcgccg
     8041 ctcagatcgc ccttgatcgc cgccaccgcg cccatctttg tcccgaacgg gttctcttcg
     8101 ctgtggccga catagaccac gtcgccatca accgtcggcg acatgttgat cccgcgccgc
     8161 gagaactgat attcccagac cttcttgccc gtccgcggct gaaaggcata gatccagccg
     8221 tcgctcgcgc cgccgatcag gagcttctgg ccgttgatcg tggcgatcac cggcgcggaa
     8281 tagatcgtgt ccggcggacg cagccgcgtg ctcacgaacc agacgacctc acccgtgcgt
     8341 ttgttgaagg ccatcagccg gtgggccggc acggccatgt cgccccagcc gatgatcacc
     8401 gaaccgagga tcacgagatc ctcaaacacg acgggaaagt tcgtgcggcc gccgtaggtc
     8461 gagagcaggc ccatctgctc gtgcagcggg atcgaccaga ggatcttgcc cgtctcgccg
     8521 tcgtagcact cgaacagccc gcacgcgccg agggcataga cgttgcccgt ttcgggatcg
     8581 ccggcgacgc tcgaccagcc gacgcgggtg tcgggcacgt cgctcgacca gacgttgtgc
     8641 cgcgattccc atttcaattc gccggtggcc gcgtcgatgc acacgacgcg ctcgccttcg
     8701 gtcggcgtgc ccggatcggc ccgcaggatc gtgtacagtt tgccgcggag gatgatcggc
     8761 gtgctccggc cgccgaactc cggcttcttc cagagcaggt tgctcccctc gccgccgtcg
     8821 ggattgatct tgtccggcag gcccgtctcg cggctgatgc cgttgtattc cggcccgcgc
     8881 cagtaaggcc aatcgagcgg atcgaccttc gcctcctggg ccagggctga tgccccgagg
     8941 aagatactcg tccaaacaac caatagcctg gacgctaacg ctaacgaggg cagggggcgt
     9001 ttcatcgtgg actcgctcgg gtgttagcag aaaatgtggc caagccggaa tcaggagcgg
     9061 ctggccggcg gtccgttcaa cgaaagggcg ggatattcag gatatgcgga cagggcgcct
     9121 gatgcaaggt tggaggtgat gcggcccgcg ctaagggact agccgcaaag gatttgggga
     9181 ggcaattact tcgccttaat cgaaccactc atccgccgga tcgaatttcg ggaagaccac
     9241 gatcagcacc ttcatccggc cgatcgcgcg gtggcgggtg agcgggcgga tcatgatgca
     9301 ggtgccgggc cgcacgtcga gcaactcgtc gttgagctgc atcttggccc ccggctcgca
     9361 ctccaggaag taataagtct cggtcagccg cttgtggtag tgtagcgcgt tgtccagggc
     9421 gatctcggtg acgtggatcg tcccgggata gtcctcgacg tccgccagcc ccctccgcgc
     9481 cgtgccgcaa gggcagggga tgccgggaag ggcggcgaaa tcgaccagtt catacggcgg
     9541 cttctcagtc acaaccattt ggttttcagt tctttggttt tcggttcttc ggttctttgg
     9601 ttctttggtt cttcggttct tttgtattgc taccgaatcg gcgtcttccc cagcaacctc
     9661 cgcagcagtc ccaattgccc cgcgtgcagc atttcatggt gcgagcagaa cagcaagccg
     9721 cccagcttcg tcgcctccac cgcgtacggc atttccaccg cctccttcaa ctgctccgcc
     9781 gtgaacgacg gcgcttcctt cagcacctgc gcgtgcacgc ggtcgagcgt ggcccgaatc
     9841 tcggccgctg ccggatactt cgccgcgtcc gcctcgggca ctgtcccgcg gctgaattgc
     9901 ttgcgaaagc tgctcgtcat caattccaaa tccaccggct gccggccccg catccgatac
     9961 aggcacagcc catactcggc catcgccaaa tgcccgattt gccaggcgac gtgcgtcggg
    10021 caaccggccg gcatggtgaa ccaatcctgc tcatcgattt ccgcaagaat cgaaagcagg
    10081 tacgagcggg cgaattcgat ctgcttgatg gcgaagtcgg cggtcatatt tcagggggcc
    10141 caaagtcaat gcggcagaac ggccaggacg gcgaacgtga tgatcggcga caaaccgatc
    10201 agcacggcgg cgacggcaat cgaccgaggg ggggcggtct ttcgcacgcg gtaagccagc
    10261 ggcgtaaaca gcaggcacag caatcccgtt gctagcgcca caaacagcag catcccggcg
    10321 accacgatga gcggatgggc tcgcg
//
LOCUS       DASRJB010000393         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3775467, whole genome shotgun sequence.
ACCESSION   DASRJB010000393 DASRJB010000000
VERSION     DASRJB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3775467"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFB80_10465"
     CDS             <1..550
                     /locus_tag="VFB80_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZN34235.1"
                     /translation="GTQVVARLAPGGNGVELFDGNVGGAGQLQVLQAFGSNAAWDLGL
                     VPAGAQSATATSTPGGDTLTAPDVNPLEVSGLFNSLLRLKNSLENFDRAEVERAINLL
                     DQDFDRVNFARGEIGARGRTLETVESHLEDEEILLKSNLADEIEIDLAEAITNLTARQ
                     AAYQASLSLSSNLFQLSLFNFL"
     gene            complement(576..>2376)
                     /locus_tag="VFB80_10470"
     CDS             complement(576..>2376)
                     /locus_tag="VFB80_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34236.1"
                     /translation="LYALAVGLLGLLHERWVFAAPLVVLALANGWLAYRQRGAAPPQP
                     LPMLRVPTDASGNNPRWYWLLAAAAPFAVIIAIGGMLPPFAYDVREYHLQAPKEWFQN
                     SRIDFLPHNIYANMPLGAELNSVWAMSLVGGDDGWWWGALAGKMAMGCYSLITAAALV
                     ALGRRLHSLAAGCIAAAVFLSTPWTVVLAVTGYNESGVMLYALLAIFALWLAGRRERQ
                     RPSGRLTLLAGFLAGSAVACKYPPALFLVVPLTAWIAVTRFLLPSPSWRRAIVGVTLF
                     LAAATAGCGLWIAKNWCQTGNPVYPLLYAEFDGLTRTPGKDRQWTKAHSPQPDASGRR
                     YSPANLLSQLAWIGWRTLLASSVLVPLVAMSVLDRRRLSLVATLGLWMLFVFATWWLF
                     THRLDRFLLLLLPAASLLAAIGALALPHPAWRAATVAVVVWGLVSQFPFATLPLDDNR
                     FFAPLAELRRDEPRLMQAVGVRLNPAQRWLNEHARSGERALLVGDAEPFELRIGAVYN
                     TCFDDCQFARLFQGKSRAERLATLRDEQIRYVVFHWTHLARYRGPANYGYTSDYVTPE
                     LVHGELVAEQHLLKPIAIDADPNKVELYEVADP"
BASE COUNT          449 a          807 c          778 g          342 t
ORIGIN      
        1 cggaactcaa gtcgtggcaa gacttgcacc gggcggcaac ggcgtcgagc tcttcgacgg
       61 caatgtcggc ggcgcgggcc agctgcaggt cctgcaagcg ttcggcagca acgccgcctg
      121 ggacctgggt ctggtgccgg ccggcgctca gtccgcgact gcgactagca cgcccggcgg
      181 cgacacgctc acagcgcccg acgtcaaccc gctggaagtc tccggcctgt tcaattccct
      241 cctccggctg aagaactcac tggagaactt cgaccgggcc gaggtcgagc gggcgatcaa
      301 cctgctggac caggatttcg accgcgtgaa cttcgcccgc ggcgagatcg gcgctcgcgg
      361 ccgcacactt gaaaccgtcg agtcgcacct ggaggacgag gaaatcctgc tcaagtcgaa
      421 cctggccgac gaaatcgaaa tcgacctcgc cgaagccatc accaatctca ccgcccgcca
      481 ggccgcctac caggcttcgc tcagcctctc gtccaatctc tttcagctct cgctgttcaa
      541 cttcttgtag cctgccgggg cggcagtatg ctgcgctaag gatcggccac ctcgtacagc
      601 tccaccttgt tcgggtcggc gtcgatcgcg atcggcttca gcaggtgctg ctcggcaacc
      661 aattcgccgt ggaccagttc cggcgtgacg tagtcgctgg tatagccata gtttgcgggg
      721 ccgcggtagc gggcgaggtg cgtccagtga aagacgacgt agcggatctg ctcatcgcgc
      781 aaagtggcga gacgctccgc gcgcgatttg ccttggaaga gccgcgcgaa ctggcaatcg
      841 tcgaagcagg tgttatagac ggctccgatc cgcagctcaa agggctccgc gtcgccgacc
      901 agtagcgccc gctcgccgct tctagcgtgc tcgttgagcc agcgctgcgc cgggttcagc
      961 cgcacgccca ccgcctgcat cagccgcggc tcgtcgcgcc gcagctcggc cagcggtgcg
     1021 aagaagcggt tgtcgtccag gggcagcgtg gcgaaaggga attggctgac gagcccccac
     1081 acgacgaccg cgaccgtcgc cgcccgccag gctggatgcg gcagggccag cgcgccgatc
     1141 gccgccagca gcgacgccgc cggcaagagc agcagcagaa agcggtcgag ccgatgcgtg
     1201 aacagccacc atgtcgcgaa gacaaagagc atccacaggc cgagcgtggc gaccaggctc
     1261 agccgccggc ggtccaagac ggacatcgcc accagcggca cgagcacgct gctggccagg
     1321 agcgtccgcc agccgatcca ggcgagctgg ctgagcagat tggccggcga atagcgccgc
     1381 ccgctggcgt ccggctgcgg cgaatgggcc ttggtccatt ggcggtcctt ccccggcgtg
     1441 cgcgtgaggc cgtcaaactc cgcgtagagc agtggataga cggggttgcc ggtttggcac
     1501 cagtttttcg ctatccatag cccgcagccg gcggtcgcag cggccaggaa cagcgtgaca
     1561 ccgacgattg cccgccgcca actggggctg gggagcagaa atcgcgtcac ggcgatccag
     1621 gcggtgagcg ggacgaccag aaacagcgcc ggaggatact tgcaggcgac cgcggagccg
     1681 gcgaggaagc ctgccagcaa cgtcagccgt ccgcttggcc tttggcgctc gcggcggccg
     1741 gccagccaga gggcgaagat tgccaggagc gcatagagca tcacgccgct ctcgttgtag
     1801 ccggtcacgg ccagcacgac ggtccacggc gttgagagga aaacagcggc ggcaatgcag
     1861 ccggcggcca gcgaatgcaa tcggcgtccc aaggcgacga gggccgcggc ggtgattagt
     1921 gaatagcaac ccatggccat cttgccggcc aacgcgcccc accaccagcc atcgtcgccg
     1981 ccgacgaggc tcatcgccca gaccgagttc agctccgccc ccagcggcat gttggcgtaa
     2041 atattgtgcg ggagaaaatc gatgcggctg ttctgaaacc actccttcgg cgcctgcaag
     2101 tgatactcgc gaacgtcgta ggcaaacggc ggcagcatcc cgccaatcgc gatgatcaca
     2161 gcgaacggcg ccgccgccgc cagcagccaa taccagcgcg ggttgttccc cgatgcgtcc
     2221 gtgggtacgc gcagcatcgg aagcggctgc ggcggcgcgg cgccgcgctg ccgataggcc
     2281 aaccatccat tggccagcgc caagaccacg agcggtgcgg caaagaccca gcgctcgtgc
     2341 agcaatccca acaatcccac cgccagggca tacagc
//
LOCUS       DASRJB010000394         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2367079, whole genome shotgun sequence.
ACCESSION   DASRJB010000394 DASRJB010000000
VERSION     DASRJB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367079"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..649
                     /locus_tag="VFB80_10475"
     CDS             155..649
                     /locus_tag="VFB80_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN34237.1"
                     /translation="MSTLHSAIAAIQFNRTRTLATLDRVLAEKDPQRALGWRPGPGRA
                     HVAWQLMHIGITEELFATERLVPGSKPEFAQLVPRFRGGSTPDDDIPTAEWIRRVLTE
                     SRAHLLATLGRFGDKDLGTIPEALQERKLALLDVLHILAWHEPHHQGQAHITLNLLKA
                     AGGP"
     gene            837..1829
                     /locus_tag="VFB80_10480"
     CDS             837..1829
                     /locus_tag="VFB80_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34238.1"
                     /translation="MSDLVRQGRRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARR
                     IKCSNSVKQFGLAFQTHHDSLGIFPDGGENWSTLAFPRTFQQGSPATAPNQNWGWGYQ
                     ILPYIEQTNVWTNGNDDTVRGAMLPIYFCSSRRKPMTIIVGPTTPNVPAAMIDYVGNG
                     GTDAFLSPVQTQVLNGIVVDIPTAAWGNGTDGVVVRKPTGGFPNRGPSLRMSMLTDGT
                     SNTLLIGEKRLRPENLGRAQAHDDQGFTSGWDRDEVCWCHIPPDIDRRGQDGFLQFGS
                     AHPSGFNAVFCDGSVRLIRYTIQSNNTVTATNPLGVWQRLCKREDGLAVDDASF"
     gene            1845..>2222
                     /locus_tag="VFB80_10485"
     CDS             1845..>2222
                     /locus_tag="VFB80_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34239.1"
                     /translation="MQWRAKWACCLLVAGVITGCSEPKFYPTRGMVRDEVGLLSAGEV
                     RFRPVSRPDLVASGKIQRDGTFTLSTPDRGEGVLEGDVQAAVIPAEGGPRIAKRYGDY
                     STADLNFTIRPRDENYFILEVRRG"
BASE COUNT          394 a          756 c          703 g          369 t
ORIGIN      
        1 cgtccgcccg tcccgccgcg tacactcagc cagcgcgcgg gcgaggccta caagcagtac
       61 cgcgcctggt accgccgcac tgtcgccccc gtgatccgcc cgatcaaaga cgccatccgc
      121 ggcaagaaac gggcggcctg acccgtctcc tgctatgagc accctccaca gcgccatcgc
      181 cgccatccag ttcaaccgca cccgcacgct ggccacgctc gaccgcgtgc tggccgaaaa
      241 ggacccgcag cgggcattgg gctggcggcc agggccgggc cgggcgcacg tcgcctggca
      301 gctgatgcac atcggcatca cggaggagct ttttgcgacc gagcgcctcg tgcccgggtc
      361 gaagcccgag ttcgcccagc tcgtgccgcg gttcagaggc ggcagcacgc ccgacgacga
      421 cattcccaca gccgaatgga tccgccgcgt gctcaccgaa tcgcgggcgc acctgctggc
      481 cacactcggc cgctttggcg acaaggacct ggggacgatt cccgaagccc tccaggagcg
      541 caagctcgcg ctgctcgacg tgctgcacat cctcgcctgg cacgagccgc accatcaggg
      601 ccaggcccac atcacgctga acctgctcaa agctgcgggt gggccgtaaa cgaatattgc
      661 agcggccggt ccgaatgtta gaatcaggat cgtgcgaggg gggttgttga tcgcatcccg
      721 gcacggcgcc aaagcgccgt ccgacttcag cggtcgaatt cttggggcgg ttccgctcgt
      781 gttccaccgg tgcgcgatcg cgcgcgccgg ttcatctcgt ttcggggaac tgagccatgt
      841 cagatctcgt gcgtcaggga cgccgggctt tcacgctggt cgagctgctg gtcgttatcg
      901 ccatcatcgg cgtgctcgtg gccctgctgc tgccggcggt ccaagcggcc cgcgaagccg
      961 cgcggcggat caagtgcagc aatagcgtca agcaattcgg actggcgttc cagacgcacc
     1021 atgactcgct gggcatcttt cccgacggcg gcgagaactg gagcacgctg gcgtttccgc
     1081 gcacgttcca gcaaggctct ccggccaccg cgcccaatca gaactggggc tggggctacc
     1141 agatcctgcc ctacatcgaa cagaccaacg tttggaccaa cggcaacgac gacacggtcc
     1201 gcggagccat gctgccgatc tatttctgtt ccagccggcg caagccgatg acgatcatcg
     1261 tcggccccac gactcctaac gtgcccgcgg cgatgatcga ctacgttggc aacggcggca
     1321 ccgacgcctt cctcagcccc gttcaaaccc aagtcttgaa tggaatcgtc gtcgatattc
     1381 ccaccgcggc ctggggaaat ggcaccgatg gcgtcgtcgt ccgcaagccc accggcggtt
     1441 ttcccaaccg cggtccctcc ctgcggatgt cgatgttgac cgacggcacg tcgaatacgc
     1501 tgctgatcgg cgagaagcgg ctgcggccgg aaaacctggg ccgggctcag gcccacgacg
     1561 accagggctt cacttccggc tgggaccgcg acgaagtttg ctggtgccat ataccgcccg
     1621 atatcgaccg ccgcggccag gacggtttcc tgcaattcgg ctcggcccat cccagcggct
     1681 tcaacgcggt cttctgcgac ggctcggtcc ggctgattcg ctacacgatc cagtcgaaca
     1741 atacggtgac agccaccaat ccgctgggcg tctggcagcg gctctgcaag cgggaggacg
     1801 gactggccgt ggatgacgcg agcttttgag ggaggcgatt tgccgtgcag tggcgagcaa
     1861 aatgggcctg ttgcctgcta gtggccggcg taatcaccgg atgcagcgag ccgaagttct
     1921 atcccacccg cggcatggtc cgggatgaag ttggcctgtt gtccgccggg gaggtccgct
     1981 tccggccagt ttcccgcccc gacctcgtgg ccagcggcaa aatccaaaga gacggcacgt
     2041 ttacactgtc aacgccggat cgcggggagg gagtgctgga aggcgacgtt caggccgccg
     2101 tgatccccgc ggagggcgga cctcgcatcg ccaagcgata tggcgactac agcacggcgg
     2161 acctgaattt cacgatcagg ccgcgggacg agaactattt cattctcgaa gtgcggcgcg
     2221 gg
//
LOCUS       DASRJB010000395         5703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_673138, whole genome shotgun sequence.
ACCESSION   DASRJB010000395 DASRJB010000000
VERSION     DASRJB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673138"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFB80_10490"
     CDS             <1..596
                     /locus_tag="VFB80_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN34240.1"
                     /translation="VDVGRIGCHLLAAPGHKGLLGQLGTGVLYVAPGVEADLLPLKQG
                     GTGTRSEEDVQPNTLPDRYESGNLNALGIVGLEAGVSHVLAENRRVEMGLLTASLVGA
                     LQAIPGLRLYGPLTGSGRLGVVSFNLPGYDPQELAALLDANWSIQSRAGIHCAPRMHA
                     ALGTSPAGTLRLSLGHFTAAADIDAAIAAIREVASAP"
     gene            692..1978
                     /locus_tag="VFB80_10495"
     CDS             692..1978
                     /locus_tag="VFB80_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34241.1"
                     /translation="MSRLGLWRLASAPLLLLAPALASADEAEARAAIAKLLDVGWATT
                     PQARVAADLQRDEVARTASGDPRALAAAWLVLLQQRRYDDGLKRIDEHLVRSPNDLNA
                     LRAKAWVQAILKNYPAALVSADKLSQQLAADPPQTEAEREDHKELIGFLGRLLGYLGG
                     PVAATVNQEQRKAAEKAILARLEASRQPLFEDAFNGVLAKFIAMTDESSDARDQAVAA
                     AVAEKDKTLAEIEADRQQAAARAKELEERRKSVQAEFQGELEQIAKEDRPLVQQLAQL
                     DSRAAVLNRDLATYQIEIDRLTRLAAGENDPARRQQLLFEADRLAIVASRIEGDLVAA
                     NRLGRGVQAQRAALATRQAQAQSNANTQVQRIDRELTDLTKRERRNDSIEKRASRPNV
                     GPTSKARALSAQATALSTYDQFPLEAAKAKLLESLR"
     gene            1984..3201
                     /locus_tag="VFB80_10500"
     CDS             1984..3201
                     /locus_tag="VFB80_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN34242.1"
                     /translation="MNRSLMISAAAIAVLSCVGFTSAEDSSGSKISFQKTVLDTLFRS
                     EGVALGDFNKDGRLDIGAGSVWYEQPAQAGGKWELRLTGEKAPEYDPHNYSHAFQTFA
                     DDLSGDGWSDLIVVDWPGSKTYWLENPQKPATVWKEHLLTPVTNNESPQFLDVDGDGK
                     LDLLAAFAPDPKNFDGPERRMGFMTRKSDPAQPWTIHPVSAPGAPSTNRYSHGLGLGD
                     INGDGRKDILAPQGWWEAPSPGPSGEWKFHAAPLGQDCAQMYAFDFDGDGDNDVLSTA
                     AHRVGMWWHEQQPEGKWTTHQIDDSITQTHSLMLADISGDGLPDFVTGKRWWAHGPQG
                     DVRPGDPAVVAWYELRRSKSGGRPEWTQHLIDHDSGVGTQFEVADVNRDGLLDVITSN
                     KKGVHYFEQARSR"
     gene            complement(3213..3428)
                     /locus_tag="VFB80_10505"
     CDS             complement(3213..3428)
                     /locus_tag="VFB80_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34243.1"
                     /translation="MFKEDSIMNEPIRRAIRHRARRAVSKDDLVRAVFESFRAQQIDL
                     RRVSLDDMKSAIVEAARAAREHSSLLA"
     gene            3921..5171
                     /locus_tag="VFB80_10510"
     CDS             3921..5171
                     /locus_tag="VFB80_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34244.1"
                     /translation="MCRFGWTSILCLAGLVAAAAGAERTWTNTAGRTMKAEFVREVDG
                     EVTFLKDGQLVTIPLTTLSERDQQIVRDLAAGKPAPEEPAVSAPAASSPAAASAPAAS
                     SPATPAAPNPAAPAGERKPLPIVNRVWTDRFGNQTTAKFVRMHGGNVVLTRANKTISL
                     PYYDLTSEDQEHLRELLTSRDQEAQIPPPPPKPVEPPPDQSPAPRPAPAGPPPGAFQP
                     PGFTPPSFPPPSFPSPPSFGGGNSRMDEFRQQAEQRHQEFLQKIEQDRQEREQRAAEG
                     RQKYQEEQERRRQEQEAARQNEIVGECLSCKGKLTRAQTDLDNCPHCGITWEFEVDSF
                     GHKRRIYNPTAGGSTSTPGAASVPTSSSPPVQLDQATVAKARQFFIIGVVVVGVLGMF
                     AVVVFVAITIAAASGAARRQRNFS"
     gene            complement(5232..>5703)
                     /locus_tag="VFB80_10515"
     CDS             complement(5232..>5703)
                     /locus_tag="VFB80_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN34245.1"
                     /translation="VQYAARMGFRTVAIARGKDKEPLARELGAVHYIDSQSADPAAEL
                     TKLGGAKVVLATVTHGPAMSATVGGLAPRGRLMVLGAAGPMEASPLTLLMGRRAIEGW
                     YSGTSIDSQETLAFSVQSGVRSMNEAYPLEKAAEAYDRMLSGKARFRVVLTTGN"
BASE COUNT         1013 a         1876 c         1897 g          917 t
ORIGIN      
        1 tggttgacgt gggccgcatc ggctgccacc tgctggctgc tcccgggcac aaggggctgc
       61 tggggcagct gggcacggga gttctctacg tcgcgccagg agtcgaggcc gacctgttgc
      121 ctctgaagca aggcggcacc gggaccagga gcgaggagga cgtgcagccg aacacgttgc
      181 ccgatcgcta tgagtcgggc aatctcaacg ccctgggcat cgtcggtttg gaagccggcg
      241 tgtcgcacgt cctggctgag aatcggcgcg tggaaatggg cctgctgacc gcttcgctgg
      301 taggcgcatt gcaggcgatc ccaggactaa ggctctacgg tcccctcacg ggttccggcc
      361 gcctgggcgt tgtcagcttc aatctgcccg gatacgaccc gcaggagctg gccgcgctgc
      421 tggacgccaa ctggtcgatc cagagccgcg ccggcatcca ctgcgccccg cgaatgcacg
      481 ccgccctcgg cacatcgccg gccggaacgc tgcggttaag cctcggacac ttcacggcgg
      541 ctgccgacat tgacgcggcc atcgcggcga tccgcgaggt tgcttcggcg ccctagtttt
      601 ccttgctccc atttggcgtc ggcgatagga tgcacgtgcg gaaatgtgcg ggccgattca
      661 ggggcggccg cggtccgcca aggagtgaac gatgtcccgt ttgggattgt ggcggctggc
      721 cagcgcgccc cttctcttgc ttgctccggc tcttgcctcg gctgacgagg ccgaagcgcg
      781 ggcggccatc gcgaagctgt tggatgtcgg ctgggcgaca acgccgcagg ctcgcgtcgc
      841 cgcagatttg cagcgcgacg aagtggctcg cacggcaagc ggtgacccgc gggccttggc
      901 ggccgcctgg ctggtgctgc tccagcagcg gcgctacgac gacgggctca agcggatcga
      961 cgaacacctg gtccgctcgc cgaacgattt gaacgccttg cgggcgaagg cctgggtgca
     1021 agcgatcttg aagaactacc cggctgcgct tgtttcggcc gacaagcttt cgcagcagct
     1081 ggcggctgac ccgccccaga ccgaggcaga gcgcgaggac cacaaggagc tgatcggttt
     1141 tctcggccga ctgctgggtt atctcggcgg ccccgtcgcc gcgacggtga accaggagca
     1201 gcgcaaggcc gccgagaagg cgattctcgc ccgcctcgaa gcgtcgcggc agccgctgtt
     1261 cgaggacgca ttcaacgggg tgctggcgaa gttcatcgcc atgaccgacg aatcgtccga
     1321 tgcccgcgac caggccgtgg ccgcagccgt ggccgagaaa gacaagacgc tggccgagat
     1381 cgaagccgac cgccagcaag ccgccgcccg cgcgaaagag ctggaggaac gccgcaagag
     1441 cgtgcaggcc gagtttcagg gcgagctgga gcagatcgcc aaggaagatc ggccgctggt
     1501 gcagcagctg gcgcagctcg attcccgcgc cgccgttctc aaccgcgacc tggccactta
     1561 ccaaattgag atcgaccgtt tgactcggct ggccgcaggg gagaacgacc cggcgcgccg
     1621 gcagcagttg ctgttcgagg ccgatcggct ggcgattgtc gcttcgcgga ttgaggggga
     1681 cctggtagct gccaaccggc tgggccgcgg tgtgcaggct cagcgggcgg ccctcgctac
     1741 gcggcaggcc caggcccagt cgaacgccaa tacgcaggtc cagcggatcg accgggagct
     1801 gactgatctc accaagcggg agcgccgcaa cgacagcatc gaaaaacgcg ccagtcggcc
     1861 gaacgtcggc ccgacgagca aggcccgcgc cctctcggcc caggccaccg cgctatcgac
     1921 ctacgatcaa ttcccgctcg aagccgccaa agcgaagctc ctcgagtctt tgcggtgaat
     1981 cctatgaatc gctcgctgat gatctctgcc gccgcaatcg ccgtgttgtc gtgcgttggt
     2041 ttcaccagcg ctgaagatag cagcggctcg aagatctcct tccagaagac cgttctcgac
     2101 acgctcttcc gcagcgaggg cgtggcgctg ggcgacttca acaaggacgg ccggctcgat
     2161 atcggcgcgg ggagtgtgtg gtacgagcag ccggcacagg ccggcggcaa atgggagctg
     2221 cgactgactg gggaaaaggc tcccgaatat gacccgcaca actacagcca cgccttccag
     2281 acgtttgcag acgacttgag cggcgacggc tggtccgacc tgatcgtcgt cgattggccc
     2341 ggctccaaga cctattggct ggagaatccg cagaagccgg ccaccgtctg gaaggagcac
     2401 ctgctgacgc cggtgaccaa caacgagagc ccgcagtttc tcgacgtcga tggcgacggc
     2461 aagctcgacc tgctcgcggc gttcgctccc gatccgaaga attttgatgg cccggagcgt
     2521 cggatgggct tcatgacgcg aaagagcgat ccggcacagc cctggacgat tcatcccgtc
     2581 tccgcgccgg gcgcgcccag caccaaccgc tattcccacg gcctgggcct tggggacatc
     2641 aacggcgacg gccggaagga cattctcgcg ccgcagggct ggtgggaagc gccttcgccc
     2701 ggcccaagcg gcgagtggaa gttccacgcg gcgccgctgg ggcaggactg cgcccagatg
     2761 tatgcgttcg atttcgacgg cgacggcgac aacgacgtgc tctcgaccgc cgcccaccgc
     2821 gtgggcatgt ggtggcacga gcagcagccg gaaggcaagt ggacgaccca ccagatcgac
     2881 gacagcatca cgcaaacgca ttcgctgatg ctcgccgaca ttagcggcga cggcctgccc
     2941 gatttcgtga cgggtaagcg gtggtgggcg cacggcccgc aaggggacgt gcgaccgggc
     3001 gatccggccg tcgtggcgtg gtacgaactc cggcgcagca aatctggcgg ccggccggag
     3061 tggacgcagc acctgatcga ccacgacagc ggcgtcggca cccagttcga agtggccgac
     3121 gtcaatcgcg acggcctgct cgacgtaatc acatcgaaca agaagggcgt ccactacttc
     3181 gagcaggcgc ggagccgcta ggcagcattc tcctaggcca acagagagct gtgctcgcgg
     3241 gcggcgcggg ccgcctcgac gatggccgac ttcatgtcgt cgagcgagac gcggcggagg
     3301 tcgatctgct gggcgcggaa cgactcgaat acggcccgta ccaggtcgtc cttcgagacg
     3361 gcgcggcggg cgcggtgccg gatggcccgg cggatcggtt cgttcatgat gctatcttcc
     3421 ttgaacataa gtcacctcac gggtgcgaat cgtcgggcga atctcagtcg ggcctcttca
     3481 atgagacgat gcggccgccg attcggttgc gacaggcatt cagacaccgg ctgaattccc
     3541 tggttcgcgg caaaaccgca tcgaattgcg ttttgcactt ggcagccgtc ctgtggcccg
     3601 tggaatgata gacagatatc gcaaggcgtc agcgccgact tgaggacgga ttcgggtcgg
     3661 ttgccggctc accacccatc gccacacgct gccgcgggcg actgccccca cgggcactcg
     3721 tgacggaact tgtcccgccc ttaggcagcg cccccgaatt tcccgaattc ggcgctggag
     3781 cgggacagtt gttccagccg taacgatttc attgacttca cagcggcccc ttggcaaact
     3841 ggagaggctt tttccgtcgg ccgaaggtga caaatctgtc aggcccggcg tgcacgtcgg
     3901 ctgcaatcag ggatgcaact atgtgccgtt tcggttggac ttcgatcctc tgtctggctg
     3961 gcctggtcgc cgctgcggct ggcgccgagc gaacctggac caatacggcc gggcgcacca
     4021 tgaaggcgga attcgtccgc gaggtggacg gcgaggtcac cttcctgaag gacggccagc
     4081 tcgtcacgat tccgctgacg acgctcagcg agcgggacca gcaaatcgtc cgcgacctgg
     4141 cggccggcaa gccggccccc gaggagccgg cggtgagcgc gcccgccgca agttcgccgg
     4201 cagcggcgag cgcgcctgcc gcaagttcgc cggcaactcc tgccgctcca aatcctgcgg
     4261 cccccgccgg tgaaagaaag ccgctgccga ttgtcaatcg ggtgtggacc gaccgttttg
     4321 gcaatcagac gacggccaag ttcgtccgca tgcacggcgg gaacgtggtc ctgacccggg
     4381 ccaataagac gatctcgctg ccctactacg atctgacatc ggaggatcag gagcatctcc
     4441 gcgagttgct aacgtcgcgc gaccaggagg cgcagattcc gccgcccccg cccaaacccg
     4501 tcgagccacc gccggaccag tcgccagcgc cgcgccccgc gccggcgggc ccgccccctg
     4561 gtgcgtttca gccgccgggc ttcactccgc ccagctttcc cccgccgagt tttcccagcc
     4621 cgccgtcctt cggcggcggc aactcgcgga tggacgaatt ccgacagcag gcagagcagc
     4681 gccaccagga attcttgcag aagatcgagc aggaccggca ggagcgcgag cagcgggccg
     4741 ccgaggggcg gcagaaatac caggaagaac aggaacgacg ccgccaggag caggaggccg
     4801 cgcggcaaaa cgagatcgtc ggcgaatgcc tgtcgtgcaa gggaaagctg acgcgggccc
     4861 agaccgatct cgacaactgc ccgcactgcg gcatcacttg ggaattcgag gtcgattcct
     4921 tcggccacaa gcggcgaatt tacaatccga cggcgggcgg ctccacctcc acgccgggcg
     4981 cggcgtcagt gcccacgagc tcctcaccgc ccgtgcagct ggatcaggcg accgtggcca
     5041 aggcccgcca gttcttcatc attggtgtgg tcgtcgtcgg cgtgctggga atgttcgccg
     5101 tggtggtgtt cgtggcgatc acgatcgccg ccgcgagtgg agccgccagg cggcagcgga
     5161 atttctccta aatagacttg cgaaaagtga cttggccgga cggatacttg tcacgatttt
     5221 gttgagcacc gtcagttgcc cgtcgtcagc acgacgcgga accgggcttt cccgctcagc
     5281 atgcggtcat aggcctcggc cgccttttcc agcgggtagg cctcgttcat cgaacgtaca
     5341 ccggattgaa cgctgaacgc cagcgtttcc tgggagtcga tggaggtccc cgaataccag
     5401 ccttcgatgg cgcggcggcc catcaggaga gtcagcggcg aagcctccat cggcccggcg
     5461 gcgcccagga ccatcagccg gccgcgcggg gcgagtccgc ccacggtcgc gctcatcgcc
     5521 ggcccgtggg tgaccgtcgc cagcacgact ttggcgccgc ccagtttggt caactcggcg
     5581 gcaggatccg cagattggct gtcgatgtag tgcactgctc ccagctcgcg ggcgagcggc
     5641 tccttgtctt tgcctcgggc gatcgccaca gtccgaaaac ccatccgcgc cgcatattgc
     5701 acg
//
LOCUS       DASRJB010000396         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2079234, whole genome shotgun sequence.
ACCESSION   DASRJB010000396 DASRJB010000000
VERSION     DASRJB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079234"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFB80_10520"
     CDS             <1..326
                     /locus_tag="VFB80_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZN34246.1"
                     /translation="SMREVVTRRFRRLRDEDEVFPDILLIDGGKGQLNAALEAFQALG
                     IEPPCLVSLAKQEEEIFRPGESESIKLSRHSAALRLLQYVRDEAHRFAQHYHHILRRK
                     SQLGE"
     gene            complement(332..748)
                     /locus_tag="VFB80_10525"
     CDS             complement(332..748)
                     /locus_tag="VFB80_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34247.1"
                     /translation="MTAIVQHNLYKGTTPRCWVRLRFAAADGSLHERELLADTGSPCA
                     VVLSQTDLALLLRAAGPNTGSNFGSLTGGWLELSMPELGLTQLVLGYGSDAVWQAVQS
                     DCPDFAGLVGLPLLRLVEYGGDSAAFWLKPTSSQIP"
     gene            complement(759..980)
                     /locus_tag="VFB80_10530"
     CDS             complement(759..980)
                     /locus_tag="VFB80_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34248.1"
                     /translation="MSSRPTREQQLKARIDATYPRGWYVAVADEKILAADADFHRLEG
                     ALRTQGYDPRSVLVVEAGIAHPEQVTIFI"
     gene            1231..>2788
                     /locus_tag="VFB80_10535"
     CDS             1231..>2788
                     /locus_tag="VFB80_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34249.1"
                     /translation="MVRPAWRAAWVVAFAGSLCAQGPGARGQEPGQLPLHERIDVLIE
                     AAAVGPLSPPCSDADFVRRVYLDLTGVIPTAEQARAFLADSAADRRAKLIDALLASPA
                     FARHLTLTLDVMLLERRPDKTALLKPWLEYLYQSIAADKPLDQLYRELLAADGADEKL
                     RPAARFLLGRDAEPNLVTRDVGRLVFGMDMQCCQCHDHPLIDDYYQRDYYGLLAFVHR
                     TSLFTDPKSKQVQLTEKADGEASYKSVFTGDSSDKAQPQLPKGAVLFVEPAFAKGEEY
                     AVKPDKAARGVPKFSRRTALAEMVAGSSEFRRNLANRIWAIVFGRGIVHPVDFHYAAN
                     PPANPELLALLADELAAGGFQLRPLVRELALTRAYQRSCDAPRSEIVNFADIAARQER
                     LNHDKEAKQAALQPLIAALAKARSDFKAAKDDDIKIAAELPKLEKAVADAKQAVAKAA
                     ADRKSADEAAAKAREQAEAVAAAAAKAAEAVTKLPDDKVLAEAAGKIAARSGELAAAA
                     DAAAKTVTEKA"
BASE COUNT          518 a          898 c          917 g          455 t
ORIGIN      
        1 ccagcatgcg cgaagtggtg acgcggcgat tccggcgctt gcgcgacgag gacgaggtat
       61 tccccgacat cctcctcatc gacggcggca aggggcagct caacgcggca ctcgaagcgt
      121 ttcaggcgct ggggatcgag ccgccgtgcc tggtctcgct ggcgaagcag gaggaggaaa
      181 tcttccgccc cggcgaatcg gagtcgatca agctcagccg ccactccgcc gccctccgcc
      241 tcctccaata cgtccgcgac gaagcccacc gcttcgccca gcattaccac cacatcctgc
      301 ggcgcaagtc acagctgggc gagtaattgt gttacggtat ttgagacgag gttggtttca
      361 gccagaaggc cgcgctgtcg ccgccatact cgacgaggcg cagcagcgga aggcccacca
      421 ggcctgcgaa gtcgggacaa tccgactgaa cggcctgcca caccgcatcg ctgccatagc
      481 ccaggacgag ctgcgtcagt ccaagctctg gcattgagag ttcgagccag ccacccgtca
      541 atgagccgaa attgctgcca gtgtttggcc ccgcggcgcg caacaggagc gcgagatcgg
      601 tctggctgag gacgacggcg caaggactgc ccgtgtcagc gaggagctcg cgttcatgca
      661 gcgaaccgtc ggcggctgca aagcgaagtc gaacccaaca gcgcggcgtc gtccctttgt
      721 agagattgtg ctggacaatg gcagtcacgc gatggctgct agatgaagat ggttacttgc
      781 tcaggatggg caatgcctgc ttccactact aagacgctgc gcggatcgta tccttgggtc
      841 cgcagcgcgc cctcgagccg gtgaaaatcg gcatcggcag caaggatctt ctcgtcggca
      901 acggcaacgt accatccccg cggataggtt gcatcgattc ttgctttcaa ttgctgctcg
      961 cgggttggtc gggacgacat ggaatacttc cagagtttgt cgtggactgt ccttaaattc
     1021 taggccgcct cggccagtag ggaacgggct gcgcgcttta cctaaaaggg ttgacggggc
     1081 caatcgccag cactattctc atagacgcgc gttgattggt cgatggatga ccggcagccg
     1141 cccccgcaga ttggatcccg cctacttggc ttgtcggcgg tgcgtcacac ggaaggacgc
     1201 gccgacagac agcccccagg agttccggtg atggttcggc cggcctggcg cgccgcttgg
     1261 gtcgttgcct tcgcgggttc gctgtgcgcg caggggccag gagccagggg tcaggagccg
     1321 ggccagctgc cgctgcatga gcggatcgat gtcttgattg aggcggcggc tgtggggccg
     1381 ctttcgccgc cgtgcagcga tgccgatttc gtccgccgcg tttatctcga cctgaccggc
     1441 gtaataccca cggctgagca ggcgcgggcg ttcctcgccg attcggccgc cgaccgacgg
     1501 gcaaagctga tcgacgcctt gctcgccagc cccgctttcg cccggcacct gacgctcacg
     1561 ctcgacgtga tgctgctcga gcggcggccc gacaagacgg cgctgctcaa gccatggctc
     1621 gaatacctct atcagtcgat cgccgccgac aagccgctgg accagctcta ccgcgaattg
     1681 ctggcggccg atggggcgga cgagaagttg cggccggccg cgcggttcct gctgggccgc
     1741 gacgccgagc cgaacctggt gacgcgcgac gtcggccgcc tggttttcgg catggacatg
     1801 cagtgctgcc agtgccacga ccatccgctg atcgacgact attaccagcg cgactactac
     1861 ggcctgttgg ccttcgtcca ccgcaccagc ctgttcaccg atcccaagag caagcaggtc
     1921 cagctcacgg agaaggctga cggcgaggcg agctacaaat cggtcttcac gggcgacagc
     1981 agcgacaagg cgcagccgca attgcccaag ggggccgtgc tgttcgtcga gccggcgttc
     2041 gcgaagggcg aagaatacgc ggtgaagccc gacaaggcgg cccgcggcgt gccgaagttc
     2101 agccggcgga cggcgctggc cgaaatggtc gccggcagca gcgaattccg ccgcaacctg
     2161 gccaaccgca tctgggcgat cgtgttcggc cgcggcatcg tgcatccggt cgatttccac
     2221 tatgcggcta atccgcccgc gaaccccgaa ctgctcgcgc tcttggccga tgagctggcc
     2281 gcgggtggct ttcaattgcg gccgctggtc cgggagctag cgctgacccg ggcctatcag
     2341 cggtcgtgcg atgctccgcg gagcgagatc gtcaatttcg ccgacatcgc cgccaggcaa
     2401 gagcggctga atcacgacaa agaggcgaag caggccgcct tgcagccgct gattgccgcg
     2461 ctggccaagg cgagaagcga tttcaaagcc gcgaaggacg acgacatcaa gattgccgcg
     2521 gaactgccca agctggagaa agcggtcgcc gatgcgaagc aggccgttgc gaaagccgcg
     2581 gccgatcgta agtccgccga cgaggccgcg gccaaagcgc gggagcaggc cgaggccgtg
     2641 gctgctgcgg ccgccaaagc agccgaggcc gtcaccaaac tgccggacga caaggtgctt
     2701 gctgaagcag cgggcaaaat cgccgcgcgg tcaggcgagt tggccgccgc cgcggatgcc
     2761 gccgccaaaa ccgtcaccga gaaggcca
//
LOCUS       DASRJB010000397        23075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2649832, whole genome shotgun sequence.
ACCESSION   DASRJB010000397 DASRJB010000000
VERSION     DASRJB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649832"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..383
                     /gene="rpmJ"
                     /locus_tag="VFB80_10540"
     CDS             270..383
                     /gene="rpmJ"
                     /locus_tag="VFB80_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZN34250.1"
                     /translation="MKVRTSIKRICENCKIVRRKGRVYVVCTNARHKQRQG"
     gene            444..827
                     /gene="rpsM"
                     /locus_tag="VFB80_10545"
     CDS             444..827
                     /gene="rpsM"
                     /locus_tag="VFB80_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZN34251.1"
                     /translation="MPRLLGVDIPNDKPIVISLRYLYGVGDKIARELCHKAGIDPAKR
                     ARDVHEEEVARLTTLLERDYTVEGPLRRQIAQDISRLRDIKCYRGMRHRLGLPVRGQR
                     TRTNARTRKGPKKTVAGKKGVKDLR"
     gene            869..1276
                     /gene="rpsK"
                     /locus_tag="VFB80_10550"
     CDS             869..1276
                     /gene="rpsK"
                     /locus_tag="VFB80_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZN34252.1"
                     /translation="MATTAAASTSTPAKKKKVRRNVTMAIAHVKATFNNTVVTITDTK
                     GDALCWASAGTCGFKGSRKSTPFAGQCAGQQAAEKAAKFGVKELEVRVKGPGSGRESA
                     ITALQTAGMTVKTIEDVTPIPHNGCRPPKRRRV"
     gene            1362..2360
                     /locus_tag="VFB80_10555"
     CDS             1362..2360
                     /locus_tag="VFB80_10555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZN34253.1"
                     /translation="MSMHIRWRGFELPSIVNVDAATLSGTYGKFTAEPFERGFGTTVG
                     NSLRRVLLSSLEGSAVTQIKIRGAQHEFTTIPGVLEDVTDIVLNVKSLVVKNHSDSTR
                     VITVEKSSTGLVTGADIQTDSDVEIINKSHVLATLTDNVPFMMEMVVENGRGYVPSTE
                     HSTGDHEIGIIPIDAVYSPVIRVRYEVEETRVGQKTNYDKLTLEIWTNGSIHPEMALV
                     EAAKVLRKHLNPFVQYAELGPKVHSPTRGSGLGIDGALDAKLNMSLAELKLSVRAMNC
                     LESEAIHTVRDLVQKTEDQLLEVRNFGETTLNEVREKLQELGLYLGMRVSPPSMVR"
     gene            2415..3002
                     /locus_tag="VFB80_10560"
     CDS             2415..3002
                     /locus_tag="VFB80_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HZN34254.1"
                     /translation="MRHRRRGRILGRAPSHRTAMLKNLASALFLTERDAELDDNEPKV
                     KGRIVTTLAKAKEVRPLVEKSITLACKALVQVEEAKAHGTTAPRNSDAWKEWRKSEKW
                     QKWAASIAPAVAARRRLNQLLGDKQAVKVLLSTVAPRFSDRRGGYTRVVKLAKPRLGD
                     AGPRAILEFVGVRDRKVERSQKPAFSDEAAETAKA"
     gene            3076..3645
                     /locus_tag="VFB80_10565"
     CDS             3076..3645
                     /locus_tag="VFB80_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34255.1"
                     /translation="MFDFTLHVRRLCDDMIARLGELAHIDMGRVAVGTCQTRRPVSHG
                     IFASLTPLRFAGGAQTTVRRGRPHGIQRVCGPDGREMLYILSIYLPRFQDLPLREKLI
                     TVLHELWHISPQFDGDIRRHGGRYHAHSHSQANYDREMGRLVDRWLGLDPPEELWSFL
                     RDDFAALAARHGRIVGLRIRRPRLLPLPQ"
     gene            complement(3657..4712)
                     /gene="gap"
                     /locus_tag="VFB80_10570"
     CDS             complement(3657..4712)
                     /gene="gap"
                     /locus_tag="VFB80_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZN34256.1"
                     /translation="MTVKIAINGFGRIGRLAYRAMLEAGIVGKGKAVDVVAVGDIVPA
                     DNLAYLLKYDTMQGRFKGTFETRKSSADKADDDLLIVNGDEMKVVSARTPAELPWKAM
                     GVDIVIESTGLFTEAEKAKGHLAAGAKKVIISAPGKGDDITIVMGVNHEKYDPKNHHI
                     LSNASCTTNCLAPVVHVLLKDGFGIDEGLMTTIHSYTATQKTVDGPSKKDWKGGRAAA
                     QNIIPSTTGAAKAVGLVCPEVKGKLTGMAFRVPTATVSAVDLTVKTAKDTSYAEISAA
                     MKRASETYLKGILDYTEDEVVSSDFIHCQASSIYDQGSGIELNKRFFKLVAWYDNEWG
                     YSCRISDLVKYVISKGL"
     gene            4851..6296
                     /locus_tag="VFB80_10575"
     CDS             4851..6296
                     /locus_tag="VFB80_10575"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZN34257.1"
                     /translation="MPTSGLALLTDLYQLTMAYGYWKLGKAEQRAAFHLYFRRPPFGG
                     GYAVTAGLGPAVEFVQAFRLETSDLDYLRSLAGNDGAPLFEAEFLRYLGELRLTCDID
                     AMPEGTVAFGQEPLVRVTGPILQCQLLETALLNIINFQTLIATKATRICAAARGEPVI
                     EFGLRRAQGIDGGLSASRAAYIGGCAGTSNVLAGKRFGIPVRGTHAHSWVMSFETELE
                     AFEGYAAVLPNNCVFLVDTYDTLEGVRRAIEVGRQLRRQGHEMVGIRLDSGDLAYLSI
                     EARQLLDEAGFPQAQIVASNDLDERIIESLKQQGAKIAVWGVGTRLATAYDQPALGGV
                     YKLGALQDARGAWQPKLKLSEQAVKVTIPGVLQVRRFESPAGLVADMIYDETRGVDPR
                     QTIVDCKDPTRQRRMAVDFAASDLLVPIVQGGQPIGSPPGLDSIRARAAGELAKLHPS
                     IRRFLNPHEYPVGIDLGLHEFRQQLIRAARE"
     gene            6339..7706
                     /locus_tag="VFB80_10580"
     CDS             6339..7706
                     /locus_tag="VFB80_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34258.1"
                     /translation="MLSNSRSLNSAALSRRELLRVGSLGGLGLALPQLLRAEQAGGGA
                     ASKADACIVIYLNGGPSHLDMWDMKPDAPVEIRGEFRPISTSLTGVQLCEHLPQLARH
                     MHRTTLVRSMNHSVNNAHAAAVYAALTGHDRGEQGGGAKPTDHPCPGAVLAKLRPAPS
                     QALPYVALPYKTQEGAGGPLQPGFLAGMLGASYDPFWILDDPNSPAFSVRNLSLPEGV
                     AADRMSGRSRLLTELDRGFAVRAERSLAAMNDFQRQAFDLLTASSVQQAFQLEVEPAA
                     TRDRYGRNIYGQSVLLARRLLEAGTRVVTMSWAPDANATWDTHGGNFQKLKSPLLPQF
                     DAACSSLIEDLADRGRLDRTLVAVLGDFGRTPKINNNAGGRDHWNACYSILLCGGGIK
                     RGFVYGASDRSGAQPVESPVSPGDILATMYRLLGVDPKKQIYDALNRPHPLVPKGRVM
                     EELIG"
     gene            complement(7808..9058)
                     /locus_tag="VFB80_10585"
     CDS             complement(7808..9058)
                     /locus_tag="VFB80_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN34259.1"
                     /translation="MRRVFGLAIVAALLLATAARAQLINDLEKRLDGAKGAAPALTIP
                     MPGYLGMVPDEEYKGDDGVKVVSVKPGGPADASGLKPGDLITSIDDKPIRTVDAMDAA
                     LTTASAGQKLKIAVRRAGGPQTLTVTLGSRPSATPAADVGEAPPAAAPPTLNPPTTSP
                     FDPLTPPAAATPPAPAASGPIRSKPLDPLAPPAAATPAETPAASPPAADPLGAPAASS
                     PAAPGGGASLGITVVPLSDEARAAYGLSLRSGALITAVKPGSPADRAGLPLGGVIAMI
                     DGRRIGTADELVSFVRAARPGQEVELGYYQGEVLRRKLVTLAPAASAAIPAAPAVTGG
                     QPPIFGNDRPILNRVERMVEGVAGGRGPSTVFDPSELATLKIQVLDLTAQVKALEERL
                     KRLEGGGAAPPAPAPGLGGFTPAP"
     gene            9217..10173
                     /locus_tag="VFB80_10590"
     CDS             9217..10173
                     /locus_tag="VFB80_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN34260.1"
                     /translation="MDSPRESTFSISPADPLAEKEVLALAALAWPQEERADLWREIRQ
                     AATADEGAVVLLAARSEDRLLAAALAHLLPGRAAVVWPPQFVTTADSSNSAIAGRLLN
                     DLAVTLGARGAVLAQTLLAAGDRQSERMRAGGFILAAELLYMAIETETIPEQPPDLPF
                     DLRCFSTGEEPALIALIDRTYQRTLDCPQIDGLRATADVVRGHRSVGQFRPELWQILL
                     VEGREAGCLLVNLHPDVGHAELVYLGLAPEVRGRGYGWLLTRQAMWLARQARVDRLVL
                     AVDAANRPAIAAYERAGLTERDRRALWYRPLVKAATGAAASS"
     gene            10531..11994
                     /locus_tag="VFB80_10595"
     CDS             10531..11994
                     /locus_tag="VFB80_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZN34261.1"
                     /translation="MRARGGKDVTKDDKEIVSALHRAVADRVGHERFAIWFGRGVRME
                     PCPAGLRIAAGDSFRLEYLRRAFRSDLLAAAKFVVGNEAALEFVVEQAAAQPSEPNLG
                     DSGPTPLLKSAAPPDNPAHLASPANGPAIDSDERQPHRPPRRQFAALNDFLASDDNRV
                     ALSAAQTAAARPGTYSPLTFVGPPGSGKTHLLEGIWRQVRQSRACQRVIYLSAEQFTN
                     QFLEALRQSGTPSFRRKYRDVELLLIDDVQFFAGKQSTLVELVHTVDTLLREGRQLVF
                     ACDRPPSELRGMGTELPARLSGGLVCTLQPADFAVRRGILQQLAGKSGVAAPPDVLNW
                     LAAQLNGDARQLAGAMHRLRAASDAHGKTIDLEFAQAALDDLVHASRRPVRLPEIVDA
                     VCDVFGVPRDELQSGSKTAAVTMPRMLVMFLARKWTRAAHSEISRSLGRKSHSTVVSA
                     QHKVTEWLADGKTVSLVTGSCRVEDAIKRIEAQLKLA"
     gene            complement(12199..14307)
                     /locus_tag="VFB80_10600"
     CDS             complement(12199..14307)
                     /locus_tag="VFB80_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN34262.1"
                     /translation="MHQILSWLVQNTLTVALLLPLVLLLARLWRFRPANQHLLWLLLL
                     VKLLTPPLVCWPVSIEMPPASSPFAGMPIGTAAHEAAASEPRQLIEPTEFAAPLVDSP
                     SAALSTGQPAAAITFAATPRAVDLPNLLPQVLVGIWLAGAAAVGAWIVRQLLQQARIL
                     RRCQEPTKTLQQWVNDSAQALQLSHVRLRVSDDVTSPFVSCLGWPTLIWPAPLAKAHE
                     LACYQAVLAHELAHLARRDHWVTWLELAAMPLWWFNPLLWYLRSRLDETRELACDALA
                     LETAGIDRRGFAELLLALSTPDRLPFSPAAPLQAGIGSAFHRRLAMLFCDRTSGRISL
                     AGLLVTAIFAAAALPGWAQAPADPKPQEVRPAQPAATTDAAAASTSSAKTADDPVEPS
                     QDPDASRPSDAARTYGSVLEIKDDILLIQLAEGASLPTNSPVFVIRITPTHSEMIGQL
                     RILKSDGKEVAAKLQPQVAKPQVGDKLWSTGRSAGANTYRRNVAGTTAQPAAEPVPSP
                     PDPPPATTEYRPSWVRVTSALNEGALTFQLEDGSRIVAHLDASGTLRMFLEKDGKRQE
                     LARSRESARTASRAATGTSSSTSPHSLPSRGTSGGASTRPAPMTAVSPPPTAARPAPP
                     SDLAVLGTNLELAKLEVEEKEILLNQAQELEKQKSISPLEVRLKEVELRKAKVRLKQA
                     QLQMDATRSRPQPGDGPQGR"
     gene            complement(14351..14752)
                     /locus_tag="VFB80_10605"
     CDS             complement(14351..14752)
                     /locus_tag="VFB80_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN34263.1"
                     /translation="MSQSRPPVSDAEREVLRELWDAGPGTVRELQQRLAAGAPSWTRS
                     TVITLLQRLEKKGYVASDRSGFAFVFRAIVSREELVVQRVRELAAELYDGQAGPLMLA
                     FAQRQRFKPEELAELRRFLDELQSQPKRKKG"
     gene            15223..15351
                     /locus_tag="VFB80_10610"
     CDS             15223..15351
                     /locus_tag="VFB80_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34264.1"
                     /translation="MAGTRGTVIRLGDRHRCVLNIDGQDEDVHVILPADEVRRSRS"
     gene            complement(15355..16593)
                     /locus_tag="VFB80_10615"
     CDS             complement(15355..16593)
                     /locus_tag="VFB80_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34265.1"
                     /translation="MRFTCLAFVLEVSFAAAALAADADWPQWRGPQASGIAAAGQSPP
                     LAWSSTENVLWKASIPGRGHGSPIVVGNSVILQTAEEDREVQSVLCFDRQTGAERWRT
                     AVHTGGLDRKGNKKTTQASATPAFDGQRLIVNFLNRGAIYTTALDLSGQQVWQTRVSD
                     FATHQGFGSSPTLFGPLVFIATDSKSGGAVAALARASGQVAWQEQRPQLPNYASATIL
                     RAAGRDQLVIHGCDVVASFDPLTGKKLWETPGSTTECVTTLVTDGERVFVSGGYPKKH
                     VQAIAADGSGRSEWENTAQVYVPSMIVHQGHLYATQDGGVAMCWDSRTGREKWKERLG
                     GTFTASLVLVGDNLLATNESGQTFVYKADPAKFQLVKENKLGDEVYASPAVCGSRIYQ
                     RVVEKSDGQRQEVLYCLGRP"
     gene            complement(16590..17090)
                     /locus_tag="VFB80_10620"
     CDS             complement(16590..17090)
                     /locus_tag="VFB80_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34266.1"
                     /translation="MRKVDEEFEGSVPRLSASTDNYEFVLNIGTEELASVCKTPRLQS
                     LAELAYRKQEPLVRVTIRDREVIAIAVPYKVKKAAPLGDGKHLDVAVLGFAATQNRCE
                     IVVEYAKRKRATVIAEAPHVQRMLEISYETKRKLMFIVVETGYVVMANLSRPAPKRTR
                     QRRASR"
     gene            complement(17156..18808)
                     /locus_tag="VFB80_10625"
     CDS             complement(17156..18808)
                     /locus_tag="VFB80_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN34267.1"
                     /translation="MYVLENPVMQRELLVNLRMVRAFVLLLLYQGLLAAVVYFAWPSE
                     TRLDLTTGSSSSQQTRRLVDLFFIGQYVIASMMAPSFAAGSITGEKERKTYEMLLASP
                     LRPAAIVLGKLVASLTHLSVLIFASLPIVMLCLPLGGVHWSEVCAAYLGLMLSVLTFG
                     MISVACSAYFQRTSAALVVSYLMILPLALLGVILWIHLADNGLFRLMLIVTVLPAVSL
                     AVCIALFFNTSALLLHPPDVGSEGKEVVDLEQEAQQAVGLVIQRDQFPDKLFAPPKRD
                     DLMEDDANPVYDKEIRSEIFSQGTLMLRLVIQVSMLLAIPFMAVCLYIWPQYAPYYIC
                     YVVIFNMLVGPVFSAGSVTSERERETLDLLLTTIITPWQILWGKLIAGLRVSSVLTLF
                     LVWPVLLACVMVSLYWSNLLSVVAYLSIVLITCLTTAMLALFCSVLFKKTSNALMTTY
                     LAIILMFCAPLAVSYFAATFFPTHAAAGVIEDLGFTSPFAAAFAIPLDMGVSGDTASA
                     AAKAALGNWPLFLAYVAFSLGLNVALLVVMIWLFNTRWRVAG"
     gene            complement(18940..19443)
                     /locus_tag="VFB80_10630"
     CDS             complement(18940..19443)
                     /locus_tag="VFB80_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZN34268.1"
                     /translation="MTQPEPLGHVPVALSPRERFVEFLQSRGMRVTQQRLALVDHVFS
                     RHEHFDADQLMEQLPGSGQAGHVSRPTVYRTLGEFVDAGLLRKFVLNGRAVYEHDYGY
                     PQHDHFHCTDCNQLFEFENEQLVQLREQLGREHHFKVSGHRLIITGVCEECQKARRRA
                     RHKMNMI"
     gene            complement(19592..20617)
                     /locus_tag="VFB80_10635"
     CDS             complement(19592..20617)
                     /locus_tag="VFB80_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HZN34269.1"
                     /translation="MRLLGASRDWTLAAARGWDRFWFTPQRPHTLALIRICGGAMLLY
                     THLIWSFDLEAFFGPHPWLTVENIRLMTQGPDGRSYAWSHLFYIQSPALLWMAHVAAL
                     AVFAMLTAGLFTRVTSILACAITLSYCHRATGAWFGLDQINAFIAMYLMVGAAGDAYS
                     LDRWLALRRGAAADAGPRIDTNIAVRLLQLHMCVIYLFGGIGKARGESWWDGTAMWMA
                     LANLEYQSLDLVWLVRYPWLLALLTHVTVFWETFYCFFVWPRLTRPICLLLAVFVHGG
                     IALALGMKTFGLVMIIGNLAFVYPEQVRATIGWLGRLIPARQPRENAAASEAPPLRKQ
                     SLAAAPR"
     gene            complement(20614..21339)
                     /locus_tag="VFB80_10640"
     CDS             complement(20614..21339)
                     /locus_tag="VFB80_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34270.1"
                     /translation="MSKRQAAGEQEPLPQRSATDSAPKLWIKLVVSAVLVFHLSAVFI
                     APLAFAADYSSPLVNAIYGVLRPYIAAMYLDHGYFFFAPNPGPSHLIDYKVEFADGRP
                     PLTGRFPDLQTERPRLRYHRHFMLTESLNNRLRYPLTPPAEPTPPALTATAEEKVLYQ
                     ANRQADERERASWRMTRRQFEAMRDSYAQHLQALHGGSRVTLTRIEHVLPSPVEVRLT
                     GRRLDDSRSYVVQPEPPRLGDAP"
     gene            complement(21554..22534)
                     /locus_tag="VFB80_10645"
     CDS             complement(21554..22534)
                     /locus_tag="VFB80_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03000 domain-containing protein"
                     /protein_id="HZN34271.1"
                     /translation="MNKLFGKVAAGAIVSLALLAWTSQANAFHHRWHGSSGGSSGGSS
                     GGSWGGSSGGWGGSSGGSSGGSWGGGYGHHRHHGGWGGSSGGWGGSSGGWGSSGGSSG
                     GWGSSGGSSGGSYYYSPAPVVDPSTIPVAPPAGGTLPPATVPPAAVPPAGTPASYSDG
                     LLAVSVPEDARIFVNGQPTSSTGGLRQYVSRNLKNGFNYAYEVRAEVVRDGKPVELVK
                     SVNVRAGETAALAFDFPATDSSQTSLTIHVPADAKVYLAGNATKATGETRIFRTTGLT
                     TGKSWDGYTVRVELDRGGKVETKEETISLKAGETREIRFDFGGDKVASSH"
BASE COUNT         4068 a         7531 c         7629 g         3847 t
ORIGIN      
        1 gatcacagcc gagcaattca ataggctgcc agcaccggac ccgacggtgg aatcagtcgc
       61 aaatgcaatc tccgtcgaaa cttacgtcga ttccaagcat cggaacgacg agcatttgag
      121 cacactcgaa acttgctgcc aggaccttca aggccgcgat acaactcagt cgcagtgagt
      181 ctcctgggcc cttgaacgtt ttcgcgaaag cccgctatac tgagtcgctt tacctggatc
      241 gatccagccg cactgcggct gagtttccta tgaaagtccg caccagcatc aaacggattt
      301 gcgagaattg caagattgtg cgccgcaagg gccgggtgta tgtcgtgtgc accaacgccc
      361 gccacaagca gcgccaggga taggcgtgcg taagtccttt cctgccatct ggtttgcgat
      421 cgaaggtcag ccggagtttt tgtatgcctc gtttgctcgg tgttgatatt ccgaacgaca
      481 agccgatcgt gatttcactg cgctacttgt atggcgtcgg cgacaagatt gctcgggagc
      541 tttgccacaa ggcagggatc gatccggcca agcgggcccg cgacgtgcac gaagaagagg
      601 tcgcccgact gaccacgctg ctggagcgag actacacggt cgaaggtccg ctgcggcggc
      661 aaatcgcgca ggacatcagc cggctgcgcg acatcaagtg ctatcgcggg atgcggcatc
      721 gcctcggcct gccggtccgc ggacagcgga cgagaaccaa cgcccggacc cgcaagggcc
      781 cgaaaaagac ggtcgccggc aagaagggcg tgaaggactt gcggtagtta atcaccattc
      841 accactcacc acctagcacc cacccgccgt ggccaccacc gcagcagcct cgacatccac
      901 gccagccaaa aagaagaagg tccgccgcaa cgtgacgatg gcaatcgcgc acgtgaaggc
      961 gacgttcaac aacacggtcg tgacgatcac cgacaccaag ggggacgccc tctgctgggc
     1021 cagcgccggc acgtgcgggt tcaaggggag ccgcaagagc acgccctttg ccgggcagtg
     1081 cgcgggccag caggccgccg agaaggccgc gaagtttggg gtcaaggaac tggaggtccg
     1141 cgtcaagggg ccgggcagcg gccgcgagag cgccatcacc gccctgcaga ccgccggcat
     1201 gaccgtgaag acgatcgaag acgtcacacc cattccgcac aacggctgcc gcccgcccaa
     1261 gcggcggcga gtttagtcat cagttatttg tcactagtca tttgtcattt ggcaggaatc
     1321 cacaagtgac aaatgaccaa cgaccaatga cactccggag catttcaatg cacattcgct
     1381 ggcgcgggtt cgagcttccc agcatcgtca acgtggatgc cgcgacgctc agcggcactt
     1441 acggcaagtt cacggcggag ccgttcgagc gcggctttgg cacgacggtc ggcaacagcc
     1501 tccgccgcgt cttgctctcc agcctggaag ggagtgccgt cacccaaatc aagattcgcg
     1561 gcgcgcaaca cgagttcacc acgatccccg gcgtgctgga ggacgtgacc gacatcgtgc
     1621 tcaacgtcaa gtcgctggtc gtgaagaacc acagcgattc gacccgcgtg atcacggtcg
     1681 agaagagctc gaccggcctg gtcaccggcg ccgacatcca gaccgattcc gacgtggaga
     1741 tcatcaacaa gagccacgtg ctggcgacct tgaccgacaa cgtgccgttc atgatggaga
     1801 tggtcgtgga gaacggccgc ggctacgtcc cttcgaccga gcacagcacc ggcgaccacg
     1861 aaatcggcat catcccgatc gacgcggttt acagcccggt gatccgcgtg cgatacgagg
     1921 tcgaagagac ccgcgtcggt cagaagacga actacgacaa gctcacgctc gagatctgga
     1981 ccaacggctc aattcacccg gagatggctc tggtcgaggc ggccaaggtt ctccgcaagc
     2041 acctcaaccc gttcgtgcag tacgccgagc tcggccccaa ggtccattcg ccgacccgcg
     2101 gctccggcct gggcatcgac ggggcgctcg acgccaagct gaacatgagc ctggccgagc
     2161 tcaagctctc tgtgcgggcc atgaactgcc tggagtcgga ggcgatccac accgtgcgcg
     2221 atctggtcca gaagaccgag gaccagctgt tggaagttcg caatttcggc gagaccacgc
     2281 tgaacgaagt ccgcgagaag ctgcaagagc tggggctgta cctcggcatg cgggtctcgc
     2341 cgccgtcgat ggtccgctaa tagtagtcat cacgctccgc gtgatgtgcc cgcacaacaa
     2401 ggctggagtt tgatatgcgt caccgcagac ggggccgaat cctcggccga gcaccgagcc
     2461 accgcacggc gatgctcaag aatctcgcca gcgccttgtt tctgaccgag cgcgacgcgg
     2521 agctcgacga caacgaaccg aaggtcaagg gccggatcgt caccacgctg gccaaggcca
     2581 aggaagtccg cccgctggtg gaaaagagca tcacgctggc ctgcaaggcc cttgtgcagg
     2641 tcgaagaggc caaggcccac ggcacgaccg cgccgcgcaa cagcgacgcc tggaaggaat
     2701 ggcggaagag cgagaaatgg cagaagtggg ccgcctcgat cgcgcccgcc gtcgccgccc
     2761 gccgccgcct caatcagttg ctgggcgaca agcaggccgt caaggtgctg ctctcgaccg
     2821 tcgccccgcg gttcagcgac cgccgcggcg gctatacccg cgtcgtcaag ctggccaagc
     2881 cccgcctggg agacgccggc ccgcgggcga tcctggagtt tgtcggcgtc cgcgaccgca
     2941 aggtcgagcg gagccagaag cctgccttca gcgacgaggc ggccgaaacc gcgaaggcct
     3001 gatagtagtc ctcacgctcc gcgtgaggtc ccgcatcacg cggagccgac ccgcgctgcg
     3061 ggtgcccggt ctactatgtt cgacttcacg ctgcacgtcc gccgcctctg cgacgacatg
     3121 atcgcgcggc tgggcgagct ggcgcacatc gacatgggcc gcgtggccgt cggcacgtgc
     3181 cagacgcgca ggcccgtgag ccacggcatt tttgcctcgc tcacgccgct gcggtttgcc
     3241 ggcggggccc agacgaccgt ccgccgtggc cggccgcacg gcatccaacg cgtctgcgga
     3301 ccggacggcc gcgaaatgct ctacatcctg agcatctacc tgccgcggtt ccaggacctg
     3361 ccgctgcgcg agaagctgat cacggtgctc cacgagctgt ggcacatcag cccccagttc
     3421 gacggcgaca tccgccgcca cggcggccgg tatcacgccc acagccattc gcaggcgaac
     3481 tacgaccgcg agatgggccg cctggtcgat cgctggctcg ggctggaccc gcccgaggag
     3541 ttgtggtcct tcctccgcga cgattttgcc gccctggccg cgcggcacgg gcggattgtc
     3601 ggtctgcgaa tccgccgccc gcggctcttg ccgctgccgc aataacttgg ggagagctac
     3661 agccccttgc tgataacgta tttcaccagg tcgctgatgc ggcagctgta gccccactcg
     3721 ttgtcgtacc aggcgaccag cttgaagaac cgcttgttga gctcgatgcc ggagccctgg
     3781 tcgtagatcg acgaggcctg gcagtggatg aagtcgctgg agacgacctc gtcctcggtg
     3841 tagtcgagga tgcccttgag gtaagtctcg ctggcccgct tcatggcggc ggagatctcg
     3901 gcgtaagacg tgtccttggc cgtcttcacg gtcagatcga cggccgagac cgtcgccgtc
     3961 ggcacgcgga aggccatgcc ggtcagcttg cccttcacct cggggcagac caggcccacg
     4021 gccttcgccg cgccggtcgt gctgggaatg atgttctggg cggccgcccg gccccccttc
     4081 caatcctttt tgctcggccc gtccaccgtt ttctgcgtgg cagtgtagga gtggatcgtg
     4141 gtcatcaggc cttcgtcgat gccaaatccg tccttgagca gaacgtggac gaccggggcg
     4201 agacaattcg tggtgcagct cgcgttcgaa aggatgtggt ggttcttggg gtcgtatttc
     4261 tcgtggttga cgcccatcac gatcgtgatg tcgtcgccct tgccgggcgc ggagatgatg
     4321 accttcttgg cgccggccgc caggtgcccc ttggctttct cggcctcggt gaacaggccg
     4381 gtcgattcga tcacgatatc gacccccatc gccttccagg gcaattcggc tggcgtcctg
     4441 gcgctgacca ccttcatctc gtcgccattg acgatcagca agtcgtcgtc ggctttgtcg
     4501 gcggaggact tgcgggtttc gaacgtgccc ttgaagcggc cctgcatggt gtcgtatttg
     4561 agcaagtagg cgaggttgtc ggcgggcaca atgtcgccga cggccaccac atcgaccgcc
     4621 ttccccttgc cgacgatgcc cgcctccagc atcgcccggt acgccagccg cccgatccgc
     4681 ccgaacccgt tgattgcaat cttgaccgtc atgactctgc tcctctttct ggttcgaatc
     4741 cgattctatc ggcggacctg ccggtgtaca acgcggtggg ttggcgcgaa gtgcaccgat
     4801 tggacccgcc gctagttagt gtatataata cactaacaag gatcgccacc atgcccactt
     4861 ccggactggc gctgctcacc gacctgtatc agctcacgat ggcctacggc tattggaagc
     4921 tgggcaaggc cgagcagcgg gccgcgtttc acctttactt ccgccggccg ccgttcggcg
     4981 gcggctacgc ggtgactgcg gggctggggc cggcggtcga gttcgtgcag gccttccgcc
     5041 tggagaccag cgatctcgac tacctgcgct ctctggcggg caacgacgga gcgccgctgt
     5101 ttgaggcgga gttcctgcgc tacctcggcg agctgcggct gacctgcgat atcgatgcga
     5161 tgcccgaggg cacggtggcg ttcggtcagg agccgctggt ccgcgtgacc ggaccgattc
     5221 tccaatgcca gctgctggag accgcgctgc tcaacatcat caatttccaa acgctgatcg
     5281 ccaccaaggc gacgcgaatc tgtgcggcgg cccgcggcga gccggtaatc gagttcggcc
     5341 tgcggcgggc ccagggaatc gacggcggcc tgtccgctag ccgggcggcc tatatcggcg
     5401 gttgcgcggg gacttcgaac gtgctggcgg gcaagcggtt cggcattccc gttcgcggaa
     5461 cccacgcgca tagctgggtg atgtcgttcg agaccgagct ggaggcgttc gagggctatg
     5521 ccgcggtgct gccgaacaac tgtgtgttcc tggtcgacac gtacgacacg ctggaaggcg
     5581 tgcggcgggc gatcgaggtc ggccgccagc tccgccggca agggcacgag atggtcggca
     5641 tccggctcga ctcgggcgac ctggcctacc tgagcatcga ggcccgccag ctgctcgacg
     5701 aagccggctt tccgcaggcg caaatcgtcg cgtcgaacga cctcgacgag cggatcatcg
     5761 aaagcctgaa gcaacaaggc gcgaaaatcg ccgtctgggg cgtgggcacc aggctggcga
     5821 cggcctacga ccagccggcg ctcggcggcg tgtataagct cggcgcgctg caagacgccc
     5881 gcggcgcctg gcaacccaag ctcaagctct cggagcaggc cgtgaaggtc accatccccg
     5941 gcgtgctcca ggtgcggcgg ttcgaatcgc ccgcgggcct cgtcgccgac atgatctacg
     6001 acgagacccg cggagtggat ccgcggcaga cgatcgtcga ttgcaaggac cccacgcggc
     6061 agcggcggat ggcggtggac ttcgcggcga gcgacttgct cgtgcccatc gttcagggcg
     6121 ggcagccgat cggctctccg cccggtctgg actcgattcg cgcccgcgcc gccggggagc
     6181 tggcgaagct gcatcccagc atccgccgct ttctcaaccc gcacgagtat ccggtcggca
     6241 tcgacttggg cctgcacgaa ttccgccagc agctgattcg ggctgcgcgc gagtaggcta
     6301 tactctggca cggatggagc ggtccccagg atgcgaatat gctatcgaac tctcgctcac
     6361 tgaattccgc agccctctcg cgccgcgaat tgctgcgcgt gggatctttg ggcggcttgg
     6421 gcctggcgct gccgcaactg ctgcgggccg agcaggccgg cggcggcgcg gcgagcaagg
     6481 ccgatgcctg catcgtgatc tacctgaacg gcgggccgag ccatctggat atgtgggaca
     6541 tgaagcccga cgcgccggtg gagattcgcg gcgaattccg cccgatctcc acttcgctga
     6601 ccggcgtcca gttgtgcgag cacttgccgc agctagcgcg gcacatgcat cggacgacgc
     6661 tcgtccgctc gatgaaccac agcgtgaaca acgcgcacgc ggctgcggtg tatgccgcgc
     6721 tcaccggcca cgaccgcggc gagcaaggcg gcggcgcgaa acccaccgat catccctgcc
     6781 cgggcgcggt gctggcgaaa ttgcggcccg cgccgagcca ggcgctgcct tacgtcgcgc
     6841 tgccctacaa gacgcaggag ggcgcgggcg gtccgctcca gcctggattt ctggcgggca
     6901 tgctgggggc cagctacgac ccgttctgga tcctcgacga ccccaattcg ccggcgttca
     6961 gcgtccgcaa cctgtcgctg cccgagggcg tggccgccga ccgcatgtcg ggccgcagcc
     7021 ggctcctgac agagctcgac cgcggcttcg ctgtccgcgc cgagcgttcg ctggccgcga
     7081 tgaacgactt tcagcggcaa gccttcgacc tgctcaccgc cagctccgtg cagcaggcgt
     7141 ttcagctcga ggtcgagccg gctgccacgc gcgaccgtta tgggcgcaat atctacgggc
     7201 aaagcgtgct cctcgcccgg cggctgctgg aggccggaac gcgggtcgtg accatgtcct
     7261 gggcgccgga cgcgaacgcc acctgggata ctcatggggg caattttcaa aagctcaaga
     7321 gtccgctctt gccgcagttc gacgccgcgt gcagcagcct gatcgaagac ctggcagacc
     7381 gcggccggct ggaccgcacg ctggtcgccg tgctgggcga tttcggccgc acgccgaaga
     7441 tcaacaacaa cgccggcggt cgcgaccact ggaacgcctg ctattcgatc ctgctctgcg
     7501 gcggcggcat caagcgcggc ttcgtgtatg gcgcgagcga ccgcagcgga gcgcagccgg
     7561 tcgagagccc ggtttcgccg ggcgacattt tggcgacgat gtaccgcctg ctgggcgtcg
     7621 atccgaaaaa acagatctac gacgcgctca atcgccccca cccactcgtc cccaaggggc
     7681 gagtgatgga ggagttgatt ggctgacgcc cggggtcggg agggtcggga gtctttgtcg
     7741 tcagcgattt cccatttgga aagtcgtcgc cgacaaagac ttccgacccc ttgccgcggc
     7801 ggcgggctta cggcgctggc gtaaatcctc caaggccggg tgcgggagca ggcggtgcgg
     7861 cgccaccgcc ttcgagccgc ttgaggcgct cctcgagggc cttgacctgg gcggtcaggt
     7921 ccagaacttg gatcttcaga gttgccaatt cgctgggatc gaacacggtc gaagggccgc
     7981 ggccgccagc cacgccctcc accatccgct cgacgcgatt gaggatgggg cggtcgttgc
     8041 caaaaatcgg cggctgaccg ccggtcacgg cgggcgctgc aggtatcgcg gcagaggcgg
     8101 ccggcgcgag cgtcaccagc ttccgccgca ggacttcgcc ctggtaatag cccagctcca
     8161 cctcttgccc cggccgcgcg gcgcggacga acgagaccag ttcgtcggcc gtaccgatgc
     8221 ggcggccgtc gatcatggcg atcacaccgc ccagcggcaa gccagcccga tcggccggcg
     8281 agccgggctt gacggccgtg atcagcgcgc cgcttcgcag cgacaggccg taggctgccc
     8341 gggcttcatc ggaaagagga accacggtga tgcccagcga ggcgccgccg ccgggcgcag
     8401 cgggactgct ggcggcggga gctcccagcg ggtcggcggc gggtggggac gccgccggag
     8461 tctccgccgg cgtcgccgca gccggcggcg caagcgggtc aagcggcttg gagcgaatcg
     8521 gcccgctggc cgcaggcgct ggcggcgttg cggcggcagg aggcgtgaga ggatcgaacg
     8581 gcgacgtggt cggcggattc agcgtcggcg gcgcggccgc gggaggtgct tcgccaacgt
     8641 ccgccgccgg agtggccgac ggccggctcc ccaaagtgac cgtcagcgtc tggggcccgc
     8701 ccgcccgccg aacggcgatt ttcagcttct ggccggccga ggcggtcgtc agcgcggcgt
     8761 ccatggcgtc cacggtgcgg atcggcttgt cgtcgatcga ggtgatcagg tcgcccggct
     8821 tgagccccga ggcatcggcc ggtccgccgg gcttgaccga gacgaccttc actccgtcgt
     8881 cccccttgta ttcctcgtcg ggaaccatgc ccaggtagcc gggcatagga atcgtgagag
     8941 cgggagcggc tcctttcgcg ccgtccagcc gcttctcgag atcgttgatc agctgagctc
     9001 gcgcggcggt tgccaacagg agcgcagcaa caatcgccaa accaaaaact cgtcgcatga
     9061 tcgcacctcc gcagcatagg ccgctcgctg tgggagcagc cggaccgcat gtcatcctga
     9121 tcgatttgaa atgagacttc tccgagtcgc cctcattata gcgctgcccg ccgaaatcgt
     9181 cgcgatcaga tcggcagatt ccgctattgt taaggcatgg attcaccgcg tgaaagcacg
     9241 ttttccatct cgccggctga tccgctggcc gaaaaagagg tgctggcgct ggcagccctc
     9301 gcctggccgc aggaggagcg ggcggacctg tggcgggaaa ttcgccaggc cgcgacggcg
     9361 gatgagggcg ccgtcgtgct gctggcggcc cgcagcgagg atcgcctgct ggcggctgcc
     9421 cttgctcacc tcttgcctgg tcgagcggcc gtcgtctggc cgccgcaatt cgtgaccacc
     9481 gccgactcga gcaactccgc catagccggc cgtttgctga acgacctggc cgtcacgctc
     9541 ggcgcgcgcg gcgcagtgct ggcccagacg ctgctcgccg ctggcgaccg gcaaagcgag
     9601 cggatgcggg ccggcgggtt cattctcgcc gcggagctgc tctacatggc catcgaaaca
     9661 gagactattc cggagcagcc ccccgatctt ccgttcgatt tacgctgctt ttcaacgggc
     9721 gaggaacccg ctttgatcgc actcatcgat cggacctacc agcggacgct cgattgccca
     9781 cagatcgacg gcctgcgagc gacggccgac gtcgtccgcg gccaccggtc agtggggcag
     9841 ttccgccccg aactctggca aattctgctg gtcgaggggc gcgaggccgg ctgcctgctc
     9901 gtcaatttgc accccgatgt cgggcatgcg gaactcgtct atctgggatt ggcgccggag
     9961 gtccgcggtc gcggctacgg ctggctgctc acgcggcagg cgatgtggct ggcgcggcag
    10021 gctcgcgtcg atcggctcgt gctggccgtg gacgcggcga acaggccggc gatcgcggcc
    10081 tacgagcgtg cggggctcac ggagcgcgac cggcgagccc tctggtaccg gccgctcgtt
    10141 aaggccgcaa ctggcgcggc ggcaagcagt taggcggcta gcgcgggaag tccgagcggc
    10201 atttgcgaca gcttgtccac accactctcc cggattgcaa aaagtcgtaa atagtcgttc
    10261 cgacgccagg cgtgcggaaa tacccgtcgt tcgcggcgag cgtgaaggct ttgcaaagaa
    10321 aacttcgcgc gacctgcaca cgaccgattt acaggcttgc tagcacggct atactcgccc
    10381 ccgcaccgcg ccactccacg gagcgcggtg caaggcagac gggtggcggg cgggggtcgt
    10441 ccgcgcagga attttactta gtgctgccgc gccagcggcg ccgcgggttc tcccgcggca
    10501 tcgtgaacac gacggaccac ggcgctgggc gtgcgcgcaa gaggcggcaa ggacgtgacc
    10561 aaggacgata aggagatcgt gtcggccctg caccgcgccg tcgccgacag agtcgggcac
    10621 gagcgatttg cgatctggtt cggccgcggc gtgcggatgg agccctgccc ggccggactt
    10681 cgcatcgcgg cgggcgattc ctttcgactg gaatatctgc ggcgggcctt ccgctccgac
    10741 ttgctcgcgg ccgcgaagtt cgttgtcggc aacgaagcgg cgctcgaatt cgtcgtcgag
    10801 caggctgccg ctcagccttc cgagccgaac ttgggcgaca gcgggcccac gccgcttctc
    10861 aagtccgccg cgccgcccga taatcctgcg catctcgcgt ctcccgcaaa tggtccagcg
    10921 atcgacagcg acgagcggca gccgcacagg ccgccgcgga gacaattcgc cgccttgaac
    10981 gacttcctcg ccagcgacga caaccgcgtg gcgctctccg cggcccagac cgcggcagcg
    11041 cggccgggca cctattcgcc gctgacgttc gtcggcccgc ccggcagcgg caaaacgcac
    11101 ctgctggaag gcatctggcg gcaagtccgc cagtcgcggg cctgccagcg ggtgatctac
    11161 ctctcggccg agcagttcac caatcagttc ctggaggccc tgcggcagtc gggcactccc
    11221 agcttccgcc gcaaatatcg cgacgtcgaa ctgctcttga tcgacgacgt gcagtttttc
    11281 gccggcaagc aatcgacgct ggtcgaattg gtgcacaccg tcgatacgct gctccgcgaa
    11341 gggcggcagc tggtcttcgc ctgcgaccgc ccgccgtcgg agctccgcgg gatgggaacg
    11401 gagctgccgg cccgcctcag tggcggcctg gtctgcacgc tgcagccggc cgactttgcg
    11461 gtgcggcgcg gcatcttgca gcagctggcg ggcaagagcg gcgtggctgc cccgccggac
    11521 gtgctcaact ggctggcggc ccagcttaac ggcgatgccc ggcagctcgc cggcgcgatg
    11581 caccgcctgc gggcggccag cgacgcccac ggcaaaacaa tcgacctgga attcgcccag
    11641 gccgcactcg acgacctggt ccacgccagc cggcggcccg tgcggctgcc tgaaatcgtc
    11701 gacgcggtct gcgacgtctt cggcgtgcca cgcgacgagc tgcagtccgg cagcaagacc
    11761 gccgccgtga cgatgccgcg aatgctggtc atgttcctgg cccgcaaatg gacccgcgcc
    11821 gcccactccg aaatcagccg ctcgctcggc cgtaagagcc acagcacggt cgtctcggcc
    11881 cagcacaaag tcaccgagtg gctcgccgac ggcaaaaccg tctcgctcgt caccggcagc
    11941 tgccgcgtcg aagacgccat caagcggatc gaagcacaac tgaagttggc ctaacgagaa
    12001 cttctgcagt ttggccgtga gtaataggcc cggagggccg acagttaata gccccgaccg
    12061 tcaggtcggg ggttcgccgt caaaagcatg tattccctca ggcccggagg gccgacacct
    12121 gcgcagccgc attcccgaac tctcgtgccg gccctccggg cctgaataca aactgcccaa
    12181 gttagttttc gacggatcct aacggccttg gggtccatcg cctggctgcg gccgtgatct
    12241 ggtggcgtcc atttgcaatt gggcttgctt cagtcgcacc ttcgccttgc gcagttcgac
    12301 ttccttgagc cgcacctcga gcggactgat ggacttttgc ttctcgagct cctgagcctg
    12361 gttgaggagg atttccttct cttccacctc gagcttagcc aactccaggt ttgtccccag
    12421 gaccgccagg tcgctgggtg gcgccggtcg tgctgcggtc ggcggcggcg aaacggccgt
    12481 catgggcgcg gggcgcgtac tcgccccgcc gctcgtgcct ctcgaaggca aggagtgcgg
    12541 tgacgtggag gaactagttc cggtcgcagc gcggctagcc gtccgggcgg attccctgga
    12601 cctggccagc tcctggcgct tgccgtcctt ctcgaggaac atgcggaggg tgccgctggc
    12661 gtccaagtgg gcgacgatcc ggcttccatc ctccagctgg aaggtcagag ctccttcgtt
    12721 cagcgcgctg gttacacgga cccaagacgg ccggtactcc gtcgtcgcgg gagggggatc
    12781 aggagggctg gggaccggct cagcagcggg ctgggctgta gtcccggcca cgttccgccg
    12841 gtaggtgttc gcaccggcgg accgcccagt ggaccagagc ttgtcaccga cttgcggttt
    12901 ggcaacctgg ggctgcaatt tcgcggcgac ttccttgcca tccgacttca ggattcgcaa
    12961 ttgaccgatc atctcgctgt gcgtcggtgt aatgcgaatc acgaagaccg gactgttggt
    13021 gggcaggctt gcgccttcgg cgagctgaat gagcaggatg tcgtccttga tctccaagac
    13081 cgatccatag gtccgggcag catcagacgg ccgactggcg tccgggtctt gggatggctc
    13141 tacgggatcg tctgcggtct ttgcgctcga cgttgacgcc gctgccgcat ccgtcgtggc
    13201 cgcaggctgc gccggcctga cttcctgtgg tttggggtca gcgggggctt gggcccagcc
    13261 gggaagcgca gcggccgcaa aaattgcggt gaccagcagc ccggccagcg agattcgtcc
    13321 gctggtgcga tcgcaaaaca gcatggcaag tctcctgtga aaggcagaac cgattcccgc
    13381 ctgcaagggc gcggcgggag agaatggcaa gcgatccggc gtcgagagcg ccagcagcag
    13441 ttccgcaaag ccgcggcggt cgattccggc ggtttccagg gcgagggcat cgcaggccag
    13501 ctcgcgggtc tcgtccagcc gcgaccgcaa gtaccacaag agcgggttga accaccacag
    13561 cggcatggcc gccagctcca gccacgtgac ccagtggtcg cgccgcgcca agtgagccaa
    13621 ttcgtgggct aacacggcct ggtaacaggc gagctcgtgg gccttggcca agggcgccgg
    13681 ccagatcagc gtcggccatc cgaggcacga aacgaacggg ctagtcacgt catcggatac
    13741 tcgcaggcgg acgtgcgaca gctgcagcgc ttgagccgag tcgttgaccc attgctgcaa
    13801 ggtcttggtc ggctcctggc accgccgcag gatgcgggcc tgctgcagca gttgccgcac
    13861 gatccaggcg ccgacggcgg ccgccccggc cagccagatt cccaccagga cctgcggcag
    13921 caggtttggc agatcgactg cgcgaggcgt tgccgcgaaa gtgatggcgg ccgccggttg
    13981 gccggtggac agtgcagcgg atggcgaatc gacgaggggc gctgcgaatt cagttggttc
    14041 aatcagttgg cgaggctcgc tggccgccgc ctcgtgcgcc gcggtcccga tcggcatccc
    14101 ggcgaagggg ctgctcgccg gaggcatctc gatcgacacc ggccaacata cgagcggcgg
    14161 cgtgaggagc ttgaccagca acagcagcca cagcaaatgc tggttggccg ggcggaatcg
    14221 ccacagcctc gccaatagca gcacgagggg cagcagcagc gctaccgtca gcgtgttttg
    14281 cacgagccag ctgagaatct ggtgcatgtc aggctcctgc gccgctgtgg cgcgccatga
    14341 cccgtcccgc tcatcccttt ttgcgcttgg gctggctttg cagctcgtcg aggaaccgcc
    14401 gcagctcggc cagttcctcg ggtttgaacc tctgccgctg tgcaaaggcc agcatcaatg
    14461 gcccggcctg gccgtcgtac aactcggcgg ccagctcgcg cacgcgctga actaccaatt
    14521 cctcgcggct gacgatcgcc cggaacacga acgcaaaacc gctgcgatcg ctggcgacgt
    14581 agcccttctt ctccagccgc tggagcagcg taatgaccgt cgagcgcgtc caactcggcg
    14641 cgcccgccgc cagtcgttgc tggagctcgc ggaccgtgcc cggcccggcg tcccacaatt
    14701 cccgcaagac ttcccgctcg gcatcggaca ccggcggacg cgactgggac atcgacattc
    14761 tcctggcgag tcacgtttac aacgtgtcaa caagcgaagc ttatctacat gctgtagata
    14821 atgcaagagg tgccgcgatc gcaccgcagg aatttcgccg ctctgccgga tcgcgactat
    14881 ttcgaacgat ctttttttcg tttgaatcaa atgagccagg agtctggtcc atttgagtca
    14941 aacgaaaaaa agattttcca gaaaagtccc ggaagggggg agctaggaaa gactgcggat
    15001 cacgcggatg gcgcgcatga gagggcggcg gctgccctat ccgcgtcatc cacgatatcc
    15061 gcggtgcaaa agtcagaatt gcgaggccgt catgggcaca tattgtgcga ctcatttccg
    15121 gtaggtacgc caatgtacgg agccatattt cgcccgacct tgtccagatg gtggccgttg
    15181 gcggtccggc cgggagatcg cgttcggatt ctgtatgggc cgatggccgg caccagggga
    15241 accgtcatcc gcctgggcga ccggcatcga tgcgtgctga atatcgacgg ccaggacgag
    15301 gacgtgcacg tcatcctgcc ggcggatgag gtgcgccgca gccgatcgta acggctacgg
    15361 gcggccgagg cagtagagca cttcctgtcg ctggccgtcg gacttttcga cgacccgctg
    15421 atagatccgg ctgccgcaga cggcggggga agcatagacc tcgtcgccga gcttgttttc
    15481 cttgacgagc tggaactttg ccggatcggc tttgtagacg aaagtctgcc ccgactcgtt
    15541 ggtggccagc aggttgtcgc cgaccaggac cagcgaggca gtgaatgtgc cgcccagccg
    15601 ctctttccat ttctcccggc cggtgcggga gtcccagcac atggcgacgc cgccgtcctg
    15661 ggtcgcgtag aggtgtccct ggtggacgat catcgacggc acatagacct gggccgtgtt
    15721 ctcccattcc gagcggccgc tgccgtcggc ggcgatcgcc tgaacgtgct tcttgggata
    15781 gccgccgctg acgaagaccc gctcgccgtc ggtgacgagc gtcgtgacgc attcggtcgt
    15841 cgagccgggc gtctcccaca gcttcttgcc ggtcagcggg tcgaagctgg ccacgacgtc
    15901 gcagccgtgg atcacgagct ggtcgcggcc ggcggcccgc aggatcgtgg ccgaggcata
    15961 gttgggcaat tgcgggcgct gctcctgcca ggcgacctgg ccgctcgcgc gcgcgagtgc
    16021 cgcgaccgcg ccgccgctct tgctgtcggt ggcgataaac accagcggcc cgaacagcgt
    16081 gggcgaggag ccgaagccct ggtgcgtggc gaaatcggag acgcgcgttt gccagacttg
    16141 ttggccggaa aggtcgaggg ccgtggtata gatcgcaccg cgattgagga agttcacgat
    16201 cagccgctgg ccgtcgaatg ccggcgtggc cgaagcctgg gtggtctttt tgttgccttt
    16261 gcggtcgagg ccgccggtat gtacggcggt ccgccaccgt tccgcgccag tctggcggtc
    16321 gaagcagagg accgactgga cctcgcggtc ctcctcggcg gtctgcagga tgaccgaatt
    16381 gccgaccacg atcggcgagc cgtgcccgcg gccgggaatg ctggccttcc agaggacgtt
    16441 ttcggtgctg ctccaggcca gcggcggcga ctggccggcc gccgcgatgc cgctagcctg
    16501 cgggccgcgc cactgcggcc aatcggcgtc ggcggcgagg gccgccgcgg caaatgaaac
    16561 ctcgagcacg aaggccaggc aggtgaatct catcgggaag ctcggcgctg gcgtgtcctt
    16621 ttgggcgcag gtcgcgacag attggccatt acgacgtagc cggtctccac aacgatgaac
    16681 atcagctttc gcttggtttc gtacgagatc tccagcatgc gttgtacgtg gggtgcctcc
    16741 gcgatgaccg tcgctcgttt gcgcttcgca tactccacga cgatctcaca ccggttctgg
    16801 gtcgcggcga atccaaggac cgccacgtcc aggtgttttc cgtccccgag cggagccgcc
    16861 ttcttgacct tatatgggac agcgatcgca atcacctccc ggtcccggat ggtgacgcga
    16921 accagcggct cctgcttgcg gtaggcgagc tcggccagac tctgcagccg gggagtctta
    16981 caaaccgacg cgagttcttc cgtgccgata ttcaggacga attcgtaatt gtccgtggaa
    17041 gccgataatc gcggcacgct gccttcaaat tcctcgtcaa cctttcgcat cgctgacctc
    17101 gtggtgcgcg gtgggcggtg cccaccagga atcgatgtcg ccgccacagc cgaccctacc
    17161 ccgcgacgcg ccagcgggtg ttgaagagcc aaatcatgac gaccaacaag gccacgttga
    17221 ggccgaggct gaaggcgacg taggccaaaa agagcggcca gttgcccagg gcggccttgg
    17281 ccgcggcgct ggcggtgtcg ccggagacgc ccatgtcgag cgggatcgca aacgcggctg
    17341 caaacgggct ggtgaagccg agatcctcga tcacgccggc ggcggcgtgc gtgggaaaga
    17401 acgtcgccgc gaagtagctc acggccaagg gagcgcagaa catgaggatg atcgccaggt
    17461 acgtcgtcat caaggcgttg gaggtcttct tgaagagcac cgagcagaac agggccagca
    17521 tcgccgtggt caggcaggtg atgagcacga tcgacaaata ggcgaccacc gagagcaggt
    17581 tcgaccagta cagcgagacc atcacgcagg ccagcagcac gggccagacc aggaacagcg
    17641 tcagcacgct cgagacgcgc agtcccgcga tcagcttgcc ccacaggatt tgccacggcg
    17701 tgatgatggt cgtcagcaac aggtcgagcg tctcgcgctc ccgttcgctg gtcacgctgc
    17761 cggccgaaaa caccgggccc acgagcatgt tgaagatcac gacatagcag atgtagtacg
    17821 gggcgtactg cggccagatg tagaggcaga ccgccatgaa cggaatcgcc aggagcatgc
    17881 tcacctggat caccagacgg agcatcagcg tcccctggct gaagatctcg ctgcggattt
    17941 ccttgtcgta gaccgggttg gcgtcgtcct ccatcaggtc gtcgcgcttg ggcggcgcga
    18001 agagcttgtc ggggaactgg tcgcgctgaa tcaccaggcc cacggcctgc tgggcctcct
    18061 gctccaggtc cacgacttcc ttgccttcgc tgccgacgtc cggcggatgc aggagcaggg
    18121 cgctggtgtt gaagaacagg gcgatgcaca cggccagcga gaccgccggc agcaccgtca
    18181 cgatcagcat cagccgaaac aggccgttgt cggccaggtg tatccacagg atcacgccga
    18241 gcagcgcgag cggcaggatc atcaggtacg agacgacgag cgccgcgctg gtccgctgga
    18301 aataggcgct gcaggccacg ctgatcatgc cgaacgtcaa gaccgagagc atcagcccca
    18361 ggtacgccgc gcagacttcc gaccagtgca cgccccccag cggcaggcag agcatcacga
    18421 tcggcagcga ggcgaaaatc agtacgctga ggtgggtcag cgaggcgacc agcttgccca
    18481 gcacgatcgc cgccggccgc agcgggctgg ccagcagcat ctcgtacgtc ttgcgttcct
    18541 tctcgccggt gatcgagccg gcggcgaagc tcggcgccat catcgaggcg atgacatact
    18601 ggccgatgaa gaacagatcg accagccgcc gcgtctgctg cgagctggag ctgcccgtgg
    18661 tcagatccag ccgcgtctcc gaaggccagg caaaatagac gaccgccgcc aagagcccct
    18721 gatagagcaa gagcagcacg aacgcccgca ccatccgcag gttcacgagc agctcgcgct
    18781 gcatgacggg gttttcgaga acgtacatgc ggaggggcta ggtgctagtg ctaggggcta
    18841 gggttaggca aggggcttcc gaatcatgat aggctgcagc gaagccggac agcaacgcgc
    18901 cgccgaccct agccctagcc cccagcccta gccccattct taaatcatgt tcatcttgtg
    18961 ccgcgcccgc cgccgggctt tctggcactc ctcgcagacg ccggtgatga tcaggcggtg
    19021 gccggagacc ttgaagtggt gctcgcggcc cagctgctcg cggagctgca cgagttgttc
    19081 gttttcgaac tcgaacaatt ggttgcagtc ggtgcagtgg aaatggtcgt gctgcggata
    19141 gccgtagtcg tgctcgtaaa cggcccggcc gttgagcacg aatttccgca gcaatcccgc
    19201 atcgacgaac tcccccagcg tgcgatagac ggtcggccgg ctgacgtggc ccgcctggcc
    19261 gctgccgggc agctgctcca tcagctggtc ggcgtcaaaa tgctcgtggc gtgaaaacac
    19321 gtgatcgacc agcgccagcc gctgctgggt gacgcgcatg ccgcggctct gcagaaactc
    19381 gacaaaacgc tcgcggggcg aaagggccac gggcacgtgg cccaagggct cgggttgggt
    19441 catacggcgc tgttccttcg gccgcctggg gacggcttat tgagactccg cgggacgcca
    19501 ggcctgccgg aattgcaggg aaaagcacga atcgggcttg gcgtcagtat caataagatt
    19561 ctaccctggc agcccgccag agcaagcggc cttaccgcgg cgcggcagcc agcgactgct
    19621 ttcgcagcgg cggggcttcg ctcgcggcgg cgttctcgcg aggttgccgg gccgggatca
    19681 gccggccaag ccagccgatc gtcgcccgga cctgctccgg atagacgaac gccaggttgc
    19741 cgatgatcat caccaggccg aaggtcttca tgccgagggc cagtgcgatg ccgccgtgca
    19801 cgaagaccgc gagcagcagg cagatcggcc gcgtcagcct gggccagacg aagaaacagt
    19861 agaacgtctc ccagaagacg gtgacgtggg tgagcagggc caagagccag ggatagcgga
    19921 ccagccagac gaggtccagc gattgatact ccaggttggc cagggccatc cacatggccg
    19981 tgccgtccca ccacgattcg ccgcgggcct tgccaatgcc gccgaacagg tagatcacgc
    20041 acatgtgcag ctgcaagagg cggaccgcaa tgttggtgtc gatgcgcggc cccgcgtccg
    20101 ctgctgctcc gcgccgcagg gccagccagc gatcgagcga gtaggcgtcg cccgccgcgc
    20161 ccaccatcag gtacatcgcg atgaaggcgt tgatctggtc gaggccgaac caggcgcccg
    20221 tcgcgcggtg gcaataggaa agcgtaatcg cgcaggccag gatcgacgtc acccgcgtga
    20281 agaggccggc ggtgagcatg gcaaagacgg ccagcgccgc cacgtgcgcc atccacagca
    20341 gggccgggga ctggatgtaa aacaggtggc tccaggcgta gctgcgtccg tcgggtcctt
    20401 gcgtcatcag gcggatgttc tcgaccgtga gccaggggtg cggtccgaag aacgcctcca
    20461 ggtcgaacga ccagatcagg tgcgtgtaga gcagcatcgc cccgccgcag atgcggatca
    20521 gcgccagcgt gtggggccgc tggggggtga accagaaccg gtcccagccg cgggcggcgg
    20581 ccagggtcca atcgcgcgag gcaccgagga gtctcatggt gcgtccccca ggcgcggcgg
    20641 ctcgggctgc acgacgtacg accgcgaatc gtcgagccgg cggccagtca gccgcacctc
    20701 aaccggcgaa ggcagcacgt gctcgatacg ggtcagcgtc acgcgtgatc cgccgtgcag
    20761 ggcctggaga tgctgggcgt aggaatcgcg catggcctcg aactgcctcc gcgtcatccg
    20821 ccagctcgcc cgctcccgct cgtcggcctg ccggttggcc tgatacagca ccttctcttc
    20881 cgccgtcgcg gtcagcgccg ggggtgtggg ttccgccggc ggcgtgagtg gatagcggag
    20941 gcggttgttg agcgattcgg tcagcatgaa gtgccggtga tagcgcaatc gcgggcgctc
    21001 ggtctgcaga tcgggaaagc ggccggtcag cggcgggcgg ccgtcggcga actcgacctt
    21061 gtaatcgatc aggtgcgagg ggccgggatt cggcgcgaag aagaaatagc cgtgatccag
    21121 gtacatcgcg gcgatgtaag gccgcagcac gccgtagatg gcgttcacca gtggcgagga
    21181 atagtccgca gcgaaagcca gcggcgcgat gaacaccgcc gacaggtgaa agaccaagac
    21241 ggcgctgacg accagcttga tccaaagctt cggcgccgaa tccgtggccg atcgttgcgg
    21301 caggggctcc tgttcccccg cggcctggcg tttggacata tcgccattca tcggccgcgc
    21361 gggagcccag tggcaaggca ttccgccagc gccggtgcga ccgtcaaggc agtttctaac
    21421 ggccctagcg gctttggccg gcaaacgaaa aagcccgcgg agcgaactcc gcgggctttc
    21481 gtttacttca gttcggtagg gtgggctgcg cccaccactt ctgattcagg tgggcacagc
    21541 ccaccctacg tgcttagtgg ctcgaggcca ccttgtcgcc gccgaagtcg aagcgaatct
    21601 cgcgcgtctc gccggctttc agcgagatgg tctcttcctt cgtttccacc ttgccgccgc
    21661 gatccagctc gacgcggacg gtatagccgt cccacgactt gccggtcgtc aggccggtcg
    21721 tgcggaagat gcgggtctcg ccggtcgcct tggtggcgtt gccagccagg tagaccttcg
    21781 cgtccgcggg cacgtgaatc gtcagcgaag tctgggacga gtccgtcgcc gggaagtcaa
    21841 aggccagcgc ggcggtctcg cccgcccgga cgttgacgct cttcaccagc tcgaccggct
    21901 tgccgtcgcg aacgacttcc gcccgcactt cataggcgta gttgaagccg ttcttcaggt
    21961 tccgcgacac atactgccgc aggccgcccg tgctcgacgt cggctggccg ttgacaaaga
    22021 tcctggcatc ttccggcacg ctcacggcca acaggccgtc gctgtacgaa gccggcgtgc
    22081 cggctggagg aaccgctgcc ggaggcaccg tggccggagg cagcgtgccg cccgcaggcg
    22141 gagcgactgg aatcgtcgag ggatcgacga cgggcgccgg cgagtagtag tagctgccgc
    22201 cgctcgagcc gccgctggaa ccccagccgc cggacgagcc gcccgaagaa ccccagccgc
    22261 cggagctgcc gccccagccg ccgctcgatc cgccccaacc gccgtgatgg cggtgatgac
    22321 cgtagccgcc gccccagcta ccgccgctgg agccgccaga gcttccgccc cagccgccgc
    22381 tggaaccgcc ccagctaccg ccgctcgagc cgccgctgga gccgccgctg gaaccgtgcc
    22441 agcgatgatg gaatgcgttg gcctgactgg tccaggccaa gagggccagc gacacaatcg
    22501 cgccagccgc gaccttgcca aacagtttgt tcatgtgagg gaatctcccg ggatgtagag
    22561 acaaaagggt ggaatcgcga gctgggaggg gttctccgac tcccaggaac ataggcaacc
    22621 gtagcatgca attcaagcag cttggggagg atttcccagg caggatatat gggttattca
    22681 ggctgactgt aacggttagc gggactgtat cgcgccgctc aaatcggcgc aaatccttgt
    22741 gagatagcgt cttgcgccag tttcaagcgc tcggacgcgg aatgccgtta gggcgacgca
    22801 tgcagcgccc ccgatcatta cgaatgagcg agctggctaa ttgatgccag cacgccgctc
    22861 tcggcccacg aactggattc tcggcgcgta agactggccg tggatgaacg cggataacgc
    22921 ggatcggacg aagctatttc cgatccgtgt catccgcgcc catccgcggc tacatttttc
    22981 ctgaaccggg attctacagt cttcgtcttc ctgctccctc acggcctgag ggagctattc
    23041 cagccctggc agctgatcga gactgcgaac gttct
//
LOCUS       DASRJB010000398         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3493903, whole genome shotgun sequence.
ACCESSION   DASRJB010000398 DASRJB010000000
VERSION     DASRJB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493903"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>2030
                     /locus_tag="VFB80_10650"
     CDS             40..>2030
                     /locus_tag="VFB80_10650"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZN34272.1"
                     /translation="MATVAAARSAGRSGKVDAVRELAKGLKIEPRFCPADVADPFDTV
                     QWELRSAQIKDENGKLMFEQKDAEIPATWTQLATNVVVSKYFYGENGTPERERSVRQL
                     IHRVTRTIADWGKADGYFADEENAERFYRELTWLCLHQHGAFNSPVWFNVGLFHINGV
                     TGTKCNWKWNPETMDVEQPENPYEFPQGSACFIQSVEDNMEAIMGLATSEAMLFKFGS
                     GTGTDLSTLRSYREKLAGGGKPSGPLSFMRVYDQIAAVVKSGGKTRRAAKMQSLKVWH
                     PDILDFIECKWKEEKKAHCLIEKGGYEANFNGEAYSSILFQNANLSVRVTDEFMQAVE
                     KNQKWTTHWVTEPNREGPSWPARELLTRMAECAWQCGDPGVQYDTTINRWHTCPNSGR
                     INASNPCSEYMFLDNTACNLASINLLKFRRPADSEGGGTFDVERFKAACRIYFIAQEI
                     LVDHASYPTAEIAKNSHLFRPLGLGYSNLGSVIMSEGYPYDSEAAYGICGSLTALLHG
                     AGNLASAEMAAVVGPFEGFHQNREPMLRVMQMHRDAVEQINPAGPKYLKDAARELWDD
                     CLALGKRVGFRNAQATVLAPTGTISFMMDCDTTGIEPDIALVKYKQLAGGGMLKIVNN
                     TVPLALKKLGYDDPTIQSICKFVDEHDTIEGAPQLAEEHL"
BASE COUNT          414 a          656 c          650 g          310 t
ORIGIN      
        1 ggccgactgc atggatgttt tctcaggagg agctctctca tggccacggt tgctgctgct
       61 cgttctgctg ggcggagtgg aaaagtggat gccgttcgcg agctggccaa ggggctgaag
      121 atcgagccgc ggttctgccc ggctgatgtg gccgacccct tcgacacggt gcagtgggag
      181 ctgcggagcg cccagatcaa ggacgagaac ggcaagctga tgttcgagca gaaggacgcc
      241 gagatcccgg cgacctggac gcagctcgcg accaacgtcg tcgtcagcaa gtacttttac
      301 ggcgaaaacg gcacgcccga acgcgagcgg agcgtgcggc aactcattca ccgcgtcacc
      361 cggacgatcg ccgactgggg caaggccgac ggctatttcg ccgacgagga aaacgccgag
      421 cggttctatc gcgagctgac ctggctctgc ctgcatcagc acggggcgtt caattcgccc
      481 gtctggttca acgtcggcct gttccatatc aacggcgtga ccggaaccaa gtgcaactgg
      541 aagtggaacc ccgaaacgat ggacgtcgag cagccggaga acccctacga atttccgcag
      601 ggctcggcgt gcttcattca gtcggtcgaa gacaacatgg aagcgatcat gggcctggcg
      661 acgagcgagg ccatgctgtt caagttcggc agcggcacgg ggacggacct gtcgacgctc
      721 cgctcgtacc gcgagaagct ggccggcggc ggcaagccca gcggcccgct gtcgttcatg
      781 cgggtctatg accagatcgc ggccgtcgtg aaaagcggcg gcaagacgcg gcgggcggcc
      841 aaaatgcagt cgctcaaggt ctggcacccc gacatcctcg acttcatcga gtgcaagtgg
      901 aaggaagaga aaaaggctca ctgcctgatc gagaagggcg gctacgaggc caacttcaac
      961 ggcgaggcct acagctcgat cctgttccag aacgccaacc tctcggtccg cgtgaccgac
     1021 gagttcatgc aggccgtcga gaagaaccag aagtggacga cgcattgggt gaccgagccc
     1081 aaccgcgaag gcccctcgtg gcccgcccgc gagctgctca cccgcatggc cgagtgcgcc
     1141 tggcagtgcg gcgacccggg cgtgcaatac gacacgacga tcaaccgctg gcacacctgc
     1201 cccaacagcg gccgcatcaa cgccagcaac ccgtgcagcg agtacatgtt cctcgacaac
     1261 acggcctgta acctggccag catcaacctg ctgaagttcc gccggccggc tgacagcgaa
     1321 ggcggcggca cgttcgacgt cgagcggttc aaggcggcct gccgcatcta tttcatcgcc
     1381 caggaaatcc tggtcgatca cgccagctat cccacggccg agatcgccaa gaacagccac
     1441 ctgttccgcc cgctgggcct gggctattcg aacctgggca gcgtgatcat gagcgagggc
     1501 tatccctacg acagcgaggc ggcctatggc atctgcggct cgctgacggc cctgctgcac
     1561 ggggcgggga acctggccag cgccgagatg gccgcggtcg tcggcccgtt cgagggcttt
     1621 catcagaacc gcgagccgat gctccgcgtg atgcagatgc accgcgatgc cgtggagcag
     1681 atcaacccgg ccggccccaa gtacctgaag gacgccgccc gcgagctgtg ggacgattgt
     1741 ctggccctcg gcaagcgcgt cggtttccgc aacgcccagg cgaccgtgct ggcccccacc
     1801 ggcacgatca gcttcatgat ggactgcgac acgacgggca tcgagcccga catcgccctg
     1861 gtcaagtaca agcagctcgc cggcggcggc atgctgaaaa tcgtcaacaa caccgtgccg
     1921 ctggctttga agaagctcgg ctacgacgac ccgacgatcc agagcatctg caaattcgtg
     1981 gatgagcacg acacgatcga gggcgccccg cagctcgccg aggagcacct
//
LOCUS       DASRJB010000399         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3777986, whole genome shotgun sequence.
ACCESSION   DASRJB010000399 DASRJB010000000
VERSION     DASRJB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3777986"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3302)
                     /locus_tag="VFB80_10655"
     CDS             complement(<1..3302)
                     /locus_tag="VFB80_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZN34273.1"
                     /translation="MSWQFWLDVGGTFTDCLARTPDGQLSRRKVLSSAVTKGRAIVVG
                     NDMIVDPARREPDGFWVGYVLRLLNDQGVVSGQSQVGLSVSGRLWLKDRSAAFDQGRQ
                     YELVSPEEAPILAIRQFLGLRLDQPIPPCSVRLGTTRGTNALLTRRGAKTAFVTTRGL
                     ADVLRIGYQNRPQLFELAIKKPQPLFELTAEIDERIAADGSILLAPSAEQVRAELSRL
                     KAAGIESLAICLLNSYANPQHEEQVAKIAGELGFAEISTSTQVSPLMKLVPRGDTTVV
                     DAYLNPVLRQYVERLAASLASGRRQPPDNVASSAIEPDREAHASRSPTELRLMTSAGG
                     LAGSGQFTGKDSILSGPAGGVVGFSRVAEAAGFTTAIGFDMGGTSTDVARYDGRFEYE
                     YETQKAGVRIAAPMLAIETVAAGGGSICRYDGMKLVVGPDSAGADPGPACYGRGGPLT
                     VTDCNLILGRLLPEHFPFPLDRAAVERRLAEVASQLASGRREPPDGVISPATPNYREA
                     DASRSPEELAEGFLRIANANMAAAIRSISLAKGYDPRDYALVAFGAAGPQHACAVAEE
                     LGITKVLIHPDAGVLSALGIGLADVVKHRAAGVYARFESLTAELRSSLDELERQVRLD
                     VIAEGIAPERTEIVRSLDLRYAGTDAAITVAEPGSGDFAASFIAQHQRLYGYVHQQRP
                     LEVVAARVQATGRSAAVLERSQALPPVRSEPADIQWRHIGGRVQAKLHHRDLLAPGSV
                     IEGPAIIVEELTTTIVDPGWQAVQLSGGELVLTQSSARTPHSHIGDERAAEIAKEAFR
                     SGKTIRELMRESEIIDSSTADPAQLEVFNNHLTAIATQMGITLRNTSMSVNVKERLDF
                     SCAIFTAEGDLVVNAPHIPVHLGAMGQTVKQVLADNPRLKPDDVIVTNDPYRGGSHLP
                     DVTVVTPVFDGESLLFFTASRAHHAEIGGVTPGSMPPFSKSLAEEGVLLRNVLAVDGG
                     QSRLDELRNLLAGGKFPSRSVEANLADITAQIAANQQGANDLRHMVERYSLPVVQAYM
                     RHIQEAAERKLRAAIAKLPPGLREFIDHLDDDTQIAVKIVVGDDHMLIDFLGTGPVSA
                     GNLNANRAIVTAA"
BASE COUNT          574 a         1118 c         1160 g          618 t
ORIGIN      
        1 gccgccgtca caatcgcccg gttcgcattg agattccccg ccgaaaccgg cccggtgcca
       61 agaaagtcaa tcagcatatg gtcgtcgccc acaacgattt tcactgcgat ctgcgtgtcg
      121 tcatccaagt gatcgataaa ctcccgcagc cccggcggca gtttggcaat tgccgcccgc
      181 aactttcgct cggccgcctc ctggatgtgc cgcatgtagg cttgcacgac cggcaacgaa
      241 tatcgctcga ccatgtgccg caagtcgttg gccccttgct ggttcgcggc aatttgtgcg
      301 gtgatgtccg caagattggc ctccacggag cggctgggaa acttcccgcc cgccaacaag
      361 ttgcgtaact cgtccagccg cgactggccg ccgtccaccg cgagcacgtt tcgcagcagc
      421 acgccttctt cggccaggct tttcgagaac ggcggcatcg acccgggcgt cacgccgccg
      481 atttcggcgt ggtgcgcccg gctggcggtg aagaagagca gcgactcgcc atcgaacacg
      541 ggcgtgacca ccgtcacgtc aggcaggtgc gagccaccgc ggtaggggtc gttggtgacg
      601 atcacgtcgt ccggcttcag ccgcgggttg tccgccagga cttgcttgac cgtttgcccc
      661 atcgcaccga ggtgcaccgg aatgtgcggc gcgttgacga ccaggtcgcc ctccgccgtg
      721 aagatggcgc agctaaagtc cagccgctcc ttgacgttca cgctcatcga cgtgttgcgg
      781 agggtgatgc ccatctgcgt ggcgatggcc gtcaggtggt tgttgaagac ttcgagctgg
      841 gcggggtcgg cggtcgagga atcgatgatt tcggattcgc gcatcagctc acggatcgtt
      901 ttgccgcttc tgaaggcttc cttggcgatt tcggcagccc gctcgtcgcc gatgtgcgag
      961 tgcggagtgc gggctgagga ctgcgtcagc accagttcgc cgccagagag ttggaccgcc
     1021 tgccagccgg gatcgacgat ggtggtcgtg agttcttcga cgatgatcgc cgggccttcg
     1081 atgaccgacc ccggtgccaa caggtcgcgg tgatggagtt tggcctgcac gcgcccgccg
     1141 atgtgccgcc attgaatatc ggcgggctca gagcggacgg ggggcagagc ctgcgatcgc
     1201 tcaagaaccg ctgcggatcg gccagtggct tgcactcgag cggcgaccac ttccagcgga
     1261 cgctgctggt ggacgtagcc gtagaggcgt tggtgctgag cgatgaagga ggcggcaaag
     1321 tcgccgctcc cgggctcggc caccgtgatt gccgcatcgg tcccggcata gcgcaggtcc
     1381 agcgagcgga cgatttcggt tctctcggga gcgatgcctt cggcgatcac atcgaggcgg
     1441 acttgccgtt cgagctcgtc cagagacgaa cggagctcgg cggtcagcga ctcaaaccgc
     1501 gcgtacacgc cagcggcacg gtgcttgacc acatcggcca gaccgatgcc cagggcgcta
     1561 agcacgcccg cgtcgggatg gatgagaact ttggtgattc ctagttcctc cgccaccgcg
     1621 caggcgtgct gcgggccggc tgcgccgaag gcgacgaggg cgtaatcgcg cggatcgtag
     1681 cctttggcga gcgagatgct gcggatggcc gcggccatgt tggcgttggc gatgcggagg
     1741 aagccctcgg cgagctcctc aggcgagcgg gaggcatcag cctcccgata gttcggcgta
     1801 gcaggagata tgacgccatc gggaggctca cgcctcccgc tcgccagttg gctcgccact
     1861 tccgccaatc gccgctcgac ggcggcgcgg tcgagtggaa agggaaaatg ctcgggcaag
     1921 aggcggccga ggatcaggtt acagtcggtg acggtgagcg ggccgccgcg gccgtagcag
     1981 gcggggccgg ggtccgcgcc ggcgctgtcg gggccgacga ccagcttcat cccgtcgtag
     2041 cggcagatcg agccgccgcc ggccgcgacc gtctcgatcg ccagcatcgg cgcggcgatc
     2101 cgcacgccgg ccttttgcgt ttcgtactcg tactcgaatc ggccgtcgta ccgcgccacg
     2161 tcggtgctcg tcccgcccat gtcgaaaccg atggctgtgg tgaaccccgc cgcttcggcg
     2221 acgcgcgaga agccaacgac gccgcccgcc ggaccgctga ggatgctgtc tttgccggtg
     2281 aattgtccgc tgccggccag gccgccggcg gaggtcatca ggcggagctc ggtgggcgag
     2341 cgggaagcgt gagcttcccg atcaggttct atcgctgacg atgcaacgtt atcgggaggc
     2401 tgacgcctcc cgctcgccag cgaggcggcc agccgctcga cgtactgccg cagcacgggg
     2461 ttcaaatagg catcgacgac ggtcgtgtcg ccgcggggaa cgagtttcat cagcggcgag
     2521 acctgcgtgc tcgtgctgat ttcggcgaag cccagctcgc cggcgatttt ggcgacctgc
     2581 tcctcgtgct gcggattggc gtagctgttg agcaggcaaa tggccagcga ttcgatcccg
     2641 gcggccttca gccgcgaaag ctcggctcgc acctgttcgg cgctgggtgc gagcaagatc
     2701 gagccgtccg cggcgattcg ttcgtcgatc tcggctgtca gctcaaacag cggctgcggc
     2761 tttttgatcg ccagctcgaa cagctggggc cggttttgat agccgatccg cagcacgtcg
     2821 gccaggccgc gggtcgtcac gaaggcggtc tttgccccgc gccgcgtgag cagcgcattg
     2881 gtcccccgcg tcgtccccag ccgcacgctg caaggcggga tcggctgatc gagccgcagc
     2941 ccgagaaact ggcggatcgc caggatcggg gcctcttcgg gagaaaccag ctcgtattgt
     3001 cgtccttgat cgaaggcggc cgatcgatcc ttaagccaca gccggccgga tacggataga
     3061 cctacctgcg actgtccgct aacgacaccc tgatcattga gcaggcgcaa cacatatccc
     3121 acccagaagc catcgggctc ccgccgcgcc ggatcaacga tcatgtcgtt gccaacaacg
     3181 atcgcccgcc ccttggtcac ggccgaactc agcacctttc gccgcgagag ctgcccgtcc
     3241 ggcgtgcgag ccaggcagtc ggtgaacgtg ccgccgacgt cgagccagaa ttgccaggac
     3301 atagacttcc tcgtttaacg cgcagccgga ggcgcaggtg tcgggcggac tcgccgttgc
     3361 ctccggctcc gagttaaacg atgctatttg ccgtcgaatg acttctcgta aacgaccgcc
     3421 ccgtccacgt cgtgatggcg aaaggtaatg gtcggtttgc cgtccaggcg
//
LOCUS       DASRJB010000400         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2369391, whole genome shotgun sequence.
ACCESSION   DASRJB010000400 DASRJB010000000
VERSION     DASRJB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369391"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFB80_10660"
     CDS             <1..1146
                     /locus_tag="VFB80_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34274.1"
                     /translation="DATLRASPRKFAPAGDCGMELSDLLPHLAARANDLCLIRSLYTD
                     QFNHHPAQLTMQCGRGELGLPVMGSWLTYGLGSESQNLPAYVVLTGGRGTSGATTLWS
                     GGYLGSHYAGVHFRTRGDAVLHLGNPPGLPRSVRRLGLDALRDLNELRLAEVGDPEIG
                     SRIAAYELAFRMQAAALELNDLSGETQETLDLYGVERPDPPIKIRRDGGPDQFRDFAT
                     NCLLARRLVERGVRFVNVFHASWDHHEDVDGELPFYTQMADQPVAALLIDLKRRGLLD
                     ETLVVWGSEFGRTPLGQGTAGRDHHPYAFSMFLAGGGTRGGYIHGRTDEIGWAPVEDP
                     LHVGDLHATMLHLLGLDHRRLTFPYQGLDQRLTTVTREARVEPRLIA"
     gene            1153..4407
                     /locus_tag="VFB80_10665"
     CDS             1153..4407
                     /locus_tag="VFB80_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34275.1"
                     /translation="MSVPSQSEESIFAEALQRTAPHERVAFVTGACAGDEPLRRRVLE
                     LLAAHDEAVGPLDAPPTWELALERPGTLVGRYKLLQMLGEGGMGFVYLAEQTQPVERR
                     VALKIIKPGMDSRQVIARFEAERQALSLMDHPNIAKVLDGGATDSGRPYFVMELVKGQ
                     PITEYCDEKHLTTRQRLELFLPVCQAIEHAHQKGIIHRDIKPSNVLVAEYDKQAVPKV
                     IDFGVAKAIGQSLTDKTMFTGLGQIVGTLEYMSPEQAKVNQLDIDTRSDIYSLGVLLY
                     ELLTGSTPFDKQRLRSAGLDEVLRIIREEEPPRPSTRLSQSGEALPSISAQRQTEPSR
                     LSRLIRGDLDWIVMKALEKNRSRRYETASSLARDLERNLNDEPVLARPPTARYRLQKF
                     VRRNRGAVLAAAAVGVILLAATAGVAGAVAWAASDRAARQSLADTGAGQALGEARGYL
                     QDRDWRQARLALDRADHYGLGTRRADLLQLASDLRADLDLVNRLERNRGGLAARTADG
                     MELDFGPTNTAYAAAFGGIDVDPFQHSPAEAGRRIRTRSVPVQLAAAIDDWATVRRFE
                     KDEPGCQRLLETAREADPDPWRNKLRDAIARQDRAALEALALESEVRNQPAVSRALLG
                     KALIDARSFQAAVATLEDARWQHPGDFWITYHLGHACQPAGRLPDAIRYITAASALAG
                     DNEMVDLKLAFLLTSQKSHQEAVVAVRRVLRSHPQMYGAHDLLWQNLQALDDKVGALS
                     AREEAFRLAPNTYKAGHVFALCNVYIERREFERVAALYAQAMELSIETPAHPAGGLFN
                     SLRQRMSDKAWSWVHPYDPTTNDPPRGIEWATRLSTTAPSSIRLSIVEQARWSEVARQ
                     QPLTLGVAHYRMGQWEQAIALLRTPDSASQHRLLRDFYLAMALNKTGRVEEGQKGFDL
                     AVRSIQGSHTRFRELWPLVAEAASVVDRPYPPTLRDPPSDAVLAPGPTLLTPADAADV
                     VPGMLVAGTNNWRCHYSWNPVPGATNYHVQVRAAHESLATVSDSSLHLYPFYNIVTFV
                     PNVGPKNVQPSWKWRVRALVNDVWTDWSEERTIWLEAPLGNDALKSDQRGSLED"
     gene            4411..>5395
                     /locus_tag="VFB80_10670"
     CDS             4411..>5395
                     /locus_tag="VFB80_10670"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HZN34276.1"
                     /translation="MKRRDFLIGTGVGGLVALGSVPTALAAGSRVREENAKPGTRDWL
                     LKNARVDASKIRCPWIEGYCSRTSAAAGDKVEIKVSTNPAAKFTLDLYRLGYYGGEGG
                     RLIERFGPLAGSPQPDPPTGDVRLRECQWETALTLTVSADWTSGVYLGKLTAQPAAKN
                     TEQREGTESYVVFIVRDDRQCDFLFQCSDTTWAAYNRWPDLWSLYDDGTPPHNWYTGP
                     GVSVSFDRPYGRYRQIFDAPLSQGSGEFLLWEFPLAYWMEQQGLDVSYISNLDTHARP
                     ERLLKARALISVGHDEYWSLAMYDNFKRAIEAGVHAAFFGGNSVDGVLDIRA"
BASE COUNT          924 a         1811 c         1793 g          867 t
ORIGIN      
        1 gacgccacgc tgcgggccag cccccgcaaa ttcgcgccgg ccggcgactg cggcatggag
       61 ctgtcggatt tgctgccgca cctggccgct cgggccaacg acctgtgcct gatccgctcg
      121 ctctacaccg accaattcaa ccatcacccg gcccagctta cgatgcagtg cggccgcggc
      181 gagctgggcc tgcccgtgat gggcagttgg ctgacctacg gcctgggcag cgagtcgcag
      241 aacctgccgg cgtacgtcgt gctcaccggc ggccgcggca ccagcggcgc gacgacgctc
      301 tggagcggcg gctatctcgg ctcgcactac gcgggcgtgc actttcgcac gcggggcgac
      361 gcggtgctgc acctgggcaa tccgcccggc ctgccgcgga gcgtgcggcg gctgggactc
      421 gacgccctcc gcgacctgaa cgagctgcgg ctggccgagg tcggcgaccc ggaaatcggc
      481 agccgcatcg ccgcctacga gctggccttc cggatgcagg cggcggccct ggagctgaac
      541 gacctgtcgg gcgagacgca ggagacgctc gacctgtacg gcgtcgagcg gcccgacccg
      601 ccgatcaaaa tccgccgcga cggcgggccg gaccagttcc gcgacttcgc gaccaactgc
      661 ctgctggccc gccggctggt cgagcggggc gtgcggttcg tgaacgtctt ccacgcctcc
      721 tgggaccacc acgaggacgt cgatggcgag ttgccgttct acacgcagat ggccgatcag
      781 ccggtggcgg ccctgctgat cgacctcaag cggcgcgggc tcttggacga gacgctcgtc
      841 gtgtggggct ccgaattcgg ccgcacgccg ctggggcaag gcacggccgg gcgggaccat
      901 cacccgtacg cgttcagcat gtttctggcc ggcggcggca cgcggggcgg ctacatccac
      961 ggccgcaccg acgagatcgg ctgggccccg gtggaagacc cgctgcacgt cggcgacctg
     1021 cacgccacca tgctgcacct gctgggcctc gaccaccgcc gcctgacctt tccctaccag
     1081 ggcctcgacc agcggctgac gacggtcacc cgcgaagccc gcgttgagcc caggctgatc
     1141 gcctagcgtg tcatgtcggt tccctcccaa tctgaagaat ccattttcgc cgaggccctg
     1201 caaaggaccg cgccgcatga acgggtcgcg ttcgtgacgg gcgcctgtgc gggcgatgag
     1261 ccgctcagac gccgcgtgct ggagttgctc gccgcccacg acgaagccgt ggggccgctc
     1321 gatgcgccac ccacctggga gctcgccctg gagcgccctg gcaccctggt cggtcgctac
     1381 aagctgctgc aaatgctcgg cgaaggcgga atgggttttg tctatctggc cgaacaaacc
     1441 cagccagtcg agcgccgcgt ggccctcaag attatcaagc cgggcatgga cagccggcaa
     1501 gtgatcgccc gcttcgaggc cgagcggcag gcgctgtcgc tgatggacca tcccaacatc
     1561 gccaaggttc tggacggcgg cgcgaccgac agcggccgcc cctacttcgt gatggaattg
     1621 gtcaaggggc agccgattac ggaatactgc gatgaaaaac acctgacgac ccgccaacgg
     1681 ctggagctgt tcctgccggt ctgccaggcg atcgagcacg cacatcagaa agggatcatc
     1741 caccgcgaca tcaagccttc caacgtcctc gtcgccgaat acgacaagca ggcggttccc
     1801 aaggtgatcg acttcggcgt ggccaaagcg atcggacagt cgctgacgga taagacgatg
     1861 ttcaccggtc tcgggcagat cgtgggaacg ctcgaataca tgagccccga gcaggccaag
     1921 gtgaaccagc tcgacatcga tacccgcagc gacatctact cgctcggcgt gttgctctat
     1981 gagctgctca cgggcagcac gccattcgac aagcagcggc tgcggtccgc cggcttggac
     2041 gaagtgctcc ggatcatccg ggaggaagaa ccgccccggc ccagcacgcg gctgtcgcaa
     2101 tccggggagg ccctaccttc gatttctgcc cagcgccaga ctgagccgtc ccggctttcc
     2161 aggctgattc gcggcgacct cgactggatc gtcatgaagg cgctcgagaa aaaccgctct
     2221 cgccgctatg aaacggccag cagcctggcg cgtgacctgg agcgcaacct caacgacgaa
     2281 cccgttctcg cccggccgcc gacggctcgc tatcgcctgc agaagttcgt gcggcgcaat
     2341 cgcggcgcgg tcctggcggc ggcggccgtc ggcgtgattc tgctggcggc cacggcgggc
     2401 gtggccggcg ccgtcgcctg ggcggcctcc gatcgcgcgg cccgccaatc gctggccgac
     2461 accggcgcag ggcaggccct gggcgaagct cgcggctact tgcaagaccg cgactggcgt
     2521 caggcccgcc tggcgctgga ccgggccgac cactacggcc tgggaacgcg ccgcgccgat
     2581 ctgctgcaat tggcgagcga cttgcgcgcc gacttggatc tcgtcaaccg gctggagcgc
     2641 aaccgcggag ggttggcggc gcgaacggct gatggcatgg agctggattt cggtccaact
     2701 aataccgcct acgcagccgc gtttggcggc atcgacgtcg atccctttca gcattcgccg
     2761 gccgaagccg gaaggcgcat tcgcactcga tccgtgccgg tgcagctggc ggctgctatc
     2821 gacgattggg cgacggtgcg gaggtttgag aaggacgaac cgggctgcca gcggctgctg
     2881 gagacggcgc gggaggctga ccccgatccg tggcgcaaca agctccgcga cgcaatcgcg
     2941 cggcaggatc gggcggccct cgaagcgctg gcattggaaa gtgaagttcg aaaccagccc
     3001 gcggtcagcc gcgcgctctt gggcaaggcc ctgatcgacg cgcggtcctt ccaagcggcc
     3061 gtggctacgc tcgaagacgc ccgctggcag catccgggcg atttctggat cacctatcac
     3121 ttgggacacg cctgccagcc ggcggggcgg ctgccggatg ccattcgcta cattacggcc
     3181 gcgtccgcgc tcgccggcga caacgagatg gtcgatctga aactggcgtt cctgctgacc
     3241 agccagaagt cgcatcaaga ggcggtcgtc gccgtgcggc gggttctgcg gtcgcacccg
     3301 caaatgtatg gagcacatga cctgttgtgg cagaatctgc aagcgctgga cgacaaagtc
     3361 ggagcgctgt cggccaggga agaggcattt cgcctggcgc caaacaccta caaagcaggg
     3421 cacgtcttcg ccctgtgcaa cgtctatatc gaacggcgcg agttcgagcg tgtcgctgcc
     3481 ctttatgcac aggccatgga attgtcgatc gagacgcccg cgcacccggc cggtggcttg
     3541 ttcaactcgc tgcgacagcg aatgagcgac aaggcctgga gttgggtgca tccttacgat
     3601 ccgacgacga acgacccgcc gcgcggcatc gagtgggcca cgcggctcag cacgacagca
     3661 ccctccagca ttcgactgag cattgtggaa caggcgcggt ggagcgaagt cgcgcgtcaa
     3721 caaccgttga cgctgggcgt ggcccattac cgcatgggcc agtgggagca ggcaattgcc
     3781 ctgctcagga cgcccgactc ggcgtcccag caccgcttgc tgcgcgattt ctacctcgcc
     3841 atggcattga acaaaacggg ccgggttgaa gaggggcaga agggcttcga cttggctgtt
     3901 cgcagcatac agggctcgca tacccgcttt cgcgagttgt ggccgctcgt ggccgaggcc
     3961 gcctcggttg tcgatcggcc ctatcctccc acgcttcgtg atccgccgtc ggatgccgtt
     4021 ctagcgcccg gtccaacgct gctcacgccg gcggacgccg cggatgtcgt gcccggtatg
     4081 ctcgttgcgg gcacgaataa ctggcgctgc cattactcct ggaaccccgt tcccggtgcg
     4141 accaattatc acgtgcaagt gcgggccgcg cacgagtctc tggcgacagt gtccgacagc
     4201 tccctccatc tctatccgtt ctacaacatc gtcacgttcg tcccgaacgt cggtccaaag
     4261 aatgtgcagc ccagttggaa atggcgggtc cgggcgctgg tcaacgatgt gtggaccgac
     4321 tggagcgaag aacgcacgat ctggcttgag gcgcccctgg ggaatgacgc tctcaagtcg
     4381 gaccagcgag gctcactgga ggattgaatc gtgaagcgac gcgactttct gatcgggact
     4441 ggcgtcggag gcctggtcgc gctgggcagc gtgccaaccg cactggctgc gggcagccgc
     4501 gtgcgggagg aaaacgcgaa gcccggcacg cgcgactggc tgctcaaaaa cgcgcgggtg
     4561 gatgccagca agatccgctg cccgtggatc gagggctatt gctcgcggac cagcgccgcc
     4621 gccggcgaca aagtcgagat caaggtcagc accaacccgg cggccaaatt cacgctcgac
     4681 ctctatcgcc tgggttatta cggcggcgag ggcggccggc tgatcgagcg gtttggaccg
     4741 ctggccggtt cgcctcagcc cgatccgccg acgggcgacg tgcggctgcg cgagtgccag
     4801 tgggagacgg ccctgacgct gacggttagc gccgattgga ccagcggcgt ttacctgggc
     4861 aagctcactg cgcagcccgc cgcgaagaac accgagcagc gcgaaggaac cgagagctac
     4921 gtcgtgttca tcgtccgcga cgaccgccag tgcgacttcc tgttccagtg ctccgatacg
     4981 acctgggcgg cctacaaccg ctggccggac ttgtggtcgc tgtacgacga cggcacgccg
     5041 ccgcacaact ggtacaccgg gcccggcgtc tcggtcagct tcgaccgccc ctatggccgc
     5101 taccggcaga ttttcgacgc gccgctgtcg caggggtcgg gcgaattcct gctgtgggag
     5161 tttccgctgg cctattggat ggaacagcag gggctcgacg tgtcgtacat ttccaacctc
     5221 gacacgcacg cccggcccga gcgactgctg aaggcgcggg ccctgatctc cgtcggccac
     5281 gacgaatact ggtcgctcgc catgtacgac aacttcaagc gggcgattga ggccggcgtc
     5341 cacgccgcgt tcttcggcgg caactcggtg gatggcgtgc tcgacatccg cgctg
//
LOCUS       DASRJB010000401         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3778275, whole genome shotgun sequence.
ACCESSION   DASRJB010000401 DASRJB010000000
VERSION     DASRJB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3778275"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFB80_10675"
     CDS             complement(<1..278)
                     /locus_tag="VFB80_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN34277.1"
                     /translation="MSVLFPLYLAGLAALTLPLILHLVRRTPKGRKEFSSLMFLAPSP
                     PRLTRRSRLDQILLLLLRLAALAFLAFAFARPFLRQEATLSLANLPSRR"
     gene            complement(278..1291)
                     /locus_tag="VFB80_10680"
     CDS             complement(278..1291)
                     /locus_tag="VFB80_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN34278.1"
                     /translation="MSRSKLSTMTASGSARQVRASFVDPAALMRIKNLQLRAKVVVEG
                     FLSGLHRSPYHGFSVEFTEYREYSPGDDLRYLDWKLFARSDRYYLKRFEDETNLRCYL
                     LVDLSKSMGFGSLDYNKAEYAKTAAATIAYFLSLQRDAVGLVTFDAAIKDYLPARFRP
                     GHLHRLMLCLEQAPAGTGTDLAAPLEQIAKTARKRGLVVLLSDLLAPIGELETKLGYL
                     RSRGHEVVVIRTLDPVEIDFPFDEAAMFHDLESGRELYVDPQLVRERYRQRFGEHRSA
                     LATVCSKLGIDLYELATNQPLELALFDLINARMRRGRQIARRQGPGRPSPARRTAAAG
                     GAR"
     gene            complement(1299..2288)
                     /locus_tag="VFB80_10685"
     CDS             complement(1299..2288)
                     /locus_tag="VFB80_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN34279.1"
                     /translation="MEQQVVEQIRGSKGRIDAELRKVIVGQADVVEQLLISLFAGGHC
                     LITGAPGLAKTLLVRSIAQIFHLKFQRIQFTPDLMPADITGTEILEQEQDGHRRMQFV
                     KGPIFGNVILADEINRTPPKTQAALLEAMQEHQVTVAGTRYTLDEPFFVLATQNPIEM
                     EGTYPLPEAQLDRFMFNVLIDYLPEDDEVAVVKQTTSKRAAAIEPIFTGDDVRRFHDV
                     VQRVPIAEDVIRYAVRLAAASRPGQSGSPGFINDWVSWGAGTRAGQYMVLGAKARALL
                     GGRNHATVDDIRALVHPTLRHRILVGYRAEAEGVTAENVISRLLETVPLPQAK"
     gene            complement(2414..3277)
                     /locus_tag="VFB80_10690"
     CDS             complement(2414..3277)
                     /locus_tag="VFB80_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZN34280.1"
                     /translation="MLKNRTRPLAVGLIAVLILSAVAVAQFSGGYGRGRGRGRSNWNR
                     EIRPEEEDRGGIPVWKNDDRFKEDVFYFVRIKYSSGGYGRGGRWDTDYPDSDFNFPYR
                     LHELTALETDPYGKIVELTDDALFDYPWIYLIEPGRMYLEEAEVLALRKYLLNGGFMM
                     VDDFWGEDEWQNFYDNIKQVFPDREPIELPLEHDIFHCVYDLKQKPQVPSIHAWWNTG
                     GTSERWDSQTPHYKGIFDDKGRMMVIICHNTDIGDGWEREGLDNEYFKQFSEKWSYPL
                     GINILMYAMTH"
     gene            complement(3285..>5425)
                     /locus_tag="VFB80_10695"
     CDS             complement(3285..>5425)
                     /locus_tag="VFB80_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN34281.1"
                     /translation="DPKVVLNSSYVEVRKRQPTYVDVYLAIGDLAFEKGDYLMAGDAY
                     QQAVKLDPLEPAAHFGLARAFAPSDSERAEAAIKAALERNPNHVDSLLLIADEQIDGE
                     QYEAAEQTLAKVAAVNPSHPRAGAYKAILAHLRNQLDKEKAFRAAALKPWPTNPEVDY
                     LIGKKLSQKYRFAEGEQYQRQALKFDPNFLPAKSQLATDLLRLGREEEGWKLAGEVYD
                     ADGYNVNAHNLVTLQENIAKFRTLEEDGLMLRMDAREADIYGRRVLDLLKRAKKGLSA
                     KYAVQLPQPIVVEMFPQQQDFAIRTFGMPGGAGFLGVCFGTVITANSPASQGASPSCW
                     EATLWHEFCHVVTLNKTKNKMPRWLSEGISVYEERQADKTWGQTITPQYRAMLLGDDL
                     VPVSQLSSAFLSPETPLHLQFAYFESSLVVEFLVEKYGLATLQRVLVDLSVGMPINES
                     LARYAGSLEALDKDFAAYARKHAEAMAKEADWTEPELPRRASAELISTWLKDHPNNYT
                     ALLRLAQQQVKEQKWEAARGTAEKLKQLYPNDESAAGPYALLAGIYRELGKTQEERAA
                     LERLAELSDDDGEMFARLVELTQRAGDHKATKKYALRWLAVNPLIPAPHRAAAAAAEG
                     LGEPALAVDCYRALLLLDPIDPAELHLKLARALSTSGDLKAAKRHALLALEETPRYRP
                     AHKLLLEIAAKLPAETPPIKPATPPAPTTP"
BASE COUNT          894 a         1726 c         1798 g         1007 t
ORIGIN      
        1 cgccggctgg gcaagttggc cagcgagagc gtcgcctcct gccgcaaaaa cggccgggcg
       61 aaggcaaacg ccaagaaggc cagcgccgct agccgtaaca agagcaaaag aatctggtcc
      121 agccggctgc ggcgcgtcag ccgcgggggc gacggcgcga ggaacatcag cgagctgaat
      181 tccttgcggc ccttgggcgt gcggcgaacc aggtgcagga tcagcggcag cgtgagcgcg
      241 gcgaggccgg ccagatacaa aggaaagagg acgctcatca gcgcgcgcct cctgccgcgg
      301 cggtgcggcg cgcggggctt ggtctgcctg ggccttgccg gcgggcgatc tgccgcccgc
      361 ggcgcatccg cgcattgatc aggtcgaaca gggccaattc cagcggctga ttcgtcgcca
      421 attcatacag gtcgattccc agcttgctgc agacggtggc cagggccgag cggtgctcgc
      481 caaagcgctg gcggtaacgc tcgcggacga gctgcggatc gacgtacagc tcgcggccgc
      541 tttccagatc gtggaacatg gcggcttcgt cgaacgggaa gtcgatctcg accggatcga
      601 gcgtgcggat gacgacgacc tcgtgcccgc gggaccgcaa gtagccgagc ttcgtctcca
      661 gttcgccgat gggggccagc aggtcggaca gcagcacgac caggccccgc ttgcgggccg
      721 tcttggcgat ctgctccagc ggcgcggcca ggtcggtgcc cgtgccggcg ggggcctgct
      781 ccaggcagag catcaatcgg tgcaaatgtc ccggccggaa ccgggcgggc aaatagtcct
      841 tgatcgcggc gtcgaaggtg accaggccga cggcgtcgcg ctgcagcgac agaaagtaag
      901 cgatcgtcgc ggctgccgtc ttcgcatact cggccttgtt gtagtcgagc gatccgaagc
      961 ccatcgactt gctcagatcg acgagcaggt agcagcggag gttcgtctcg tcctcgaacc
     1021 gcttgaggta gtagcggtcg gagcgggcga agagtttcca atcgagatac cgcaggtcgt
     1081 cgccaggcga gtattcgcgg tactcggtga actcgaccga gaagccgtga taggggctgc
     1141 gatgcaagcc gctcaaaaag ccctcgacga caaccttggc ccgcagctgc agattcttga
     1201 tccgcatcaa ggccgcgggg tcgacgaacg aagcccgaac ctgccgggcg gaaccggacg
     1261 ccgtcatcgt gctcagcttc gagcggctca tgagcgactt acttagcttg cggcagcggc
     1321 accgtctcca gcagccggct gatcacgttc tcggcggtga cgccttcggc ctcggcgcgg
     1381 tagccgacga gaatgcggtg ccgcagcgtg ggatggacga gggcccggat gtcatcgacc
     1441 gtagcgtggt tgcggccgcc caggagggcc cgggccttcg cgcccaagac catatactgc
     1501 ccggcccgcg tgcccgcgcc ccagctcacc cagtcgttga taaagccggg cgagccgctc
     1561 tggccgggtc ggctggcggc ggccaggcgg acggcgtagc ggatcacgtc ctcggcaatc
     1621 ggcacgcgct gcacgacgtc gtgaaaccgc cgcacatcgt cgcccgtgaa gatcggctcg
     1681 atcgccgcgg cccgcttgga agtggtctgc ttgacgacgg ccacctcgtc gtcttccggc
     1741 aggtaatcga tcagcacgtt gaacatgaag cggtcgagct gggcctcggg cagcggataa
     1801 gtgccctcca tctcgatcgg gttctgcgtg gccaggacga agaacggctc gtccagcgtg
     1861 tagcgcgtgc cggccacggt gacctggtgc tcctgcatcg cctcgagcag ggcggcctgg
     1921 gtcttggggg gcgtgcggtt gatttcgtcg gccaggatca cgttgccgaa gatcgggccc
     1981 ttgacgaact gcatccggcg gtggccgtcc tgctcctgct ccaggatttc cgtgcccgtg
     2041 atgtcggccg gcatcaggtc gggcgtaaac tggatccgct ggaacttcag gtggaagatc
     2101 tgggcgatcg agcggaccag cagcgtcttc gccagacccg gcgcgccggt gatgaggcaa
     2161 tgcccgcctg cgaacaggct gatgagcagc tgctccacga cgtcggcctg gcccacgatg
     2221 accttgcgga gctccgcgtc gatgcgtccc ttgctgccgc gaatctgctc caccacctgc
     2281 tgttccattt cataagcatc aactgccacg cgtaaccacc cttctggttg tcacttgtca
     2341 cttctcattg gtcgcttgtc attggtcatt tatcgctgca atctccgcac gagtgaccaa
     2401 tgacaagtga catctagtgc gtcatcgcat acatcaaaat gttgattccc agcggatacg
     2461 accacttctc ggaaaactgc ttgaaatact cgttgtcgag accttcccgc tcccagccgt
     2521 cgccgatgtc ggtgttgtgg cagatgatga ccatcatccg gcccttgtcg tcgaagatgc
     2581 ctttgtagtg cggcgtttgc gaatcccagc gctcggatgt cccgccggtg ttccaccagg
     2641 cgtggatgct gggaacctgc ggcttctgct tgaggtcgta gacgcagtgg aagatgtcgt
     2701 gctccagcgg cagctcgatc ggctcgcggt cgggaaagac ctgtttgatg ttatcgtaga
     2761 agttctgcca ctcgtcttcg ccccagaagt cgtccaccat catgaagccg ccgttgagca
     2821 ggtacttccg cagggccagc acctcggcct cttcgaggta catgcggccc ggctcgatca
     2881 gatagatcca gggatagtcg aagagcgcgt cgtcggtcag ctcgacgatc ttaccgtagg
     2941 ggtcggtctc cagggcggtc agctcgtgca ggcggtaggg gaaattgaaa tcgctgtcgg
     3001 gatagtccgt gtcccacctg ccgccgcgac cgtagccccc ggaggaatac ttgatgcgga
     3061 cgaagtagaa gacgtcctcc ttgaaccgat cgtcgttctt ccacacgggg attccgccgc
     3121 gatcctcctc ttccgggcga atctcgcggt tccagttcga gcgaccacgg ccccgcccgc
     3181 ggccgtatcc accgctgaat tgagccacgg cgaccgcgga cagaatcagc acggcgatca
     3241 ggccgacggc gagcgggcgt gttcggttct taagcacggc tgcctcaggg agtggtagga
     3301 gcgggcggag ttgcgggttt gatcggcgga gtttctgcgg gcagcttggc cgcgatctcc
     3361 agcagcaatt tgtgggccgg gcggtagcgg ggcgtctctt cgagggccaa gagcgcgtgc
     3421 cgcttggcgg ccttgaggtc gccgctggtc gagagggccc tcgccagctt caagtgcaac
     3481 tcggccgggt cgatcggatc gagcaggagc agcgcccggt agcagtcgac ggccagcgcc
     3541 ggttcgccca agccttcagc cgcggcggcc gctgcccggt gcggcgcggg aatcagcgga
     3601 ttgacggcga gccagcggag ggcgtacttc ttcgtggcct tgtggtcgcc ggccctttgc
     3661 gtgagctcga ccaggcgggc gaacatctcg ccgtcgtcgt ccgacagctc cgccagccgc
     3721 tcgagcgccg cccgctcttc ctgggtcttg ccaagctctc tatatattcc ggccaacagg
     3781 gcatacgggc cggctgcact ttcgtcgttc ggatagagct gcttcagttt ttccgccgta
     3841 ccgcgcgcgg cctcccattt ctgctccttg acctgctgct gggccaggcg cagcagggcg
     3901 gtgtagttgt tcggatggtc cttgagccag gtcgaaatca gctcggcact ggcccgccgc
     3961 ggcagctcgg gctcggtcca gtcggcctct ttggccatcg cctcggcgtg cttgcgggcg
     4021 taggcggcaa aatccttgtc gagggcctcc agcgagccgg cgtagcgggc cagcgattcg
     4081 ttgatgggca tccccacgct cagatcgacg agcacccgct gcagcgtggc gaggccgtac
     4141 ttctcgacca aaaactcgac gaccagcgag gactcgaaat aggcgaactg caggtgcagc
     4201 ggcgtctcgg ggctgaggaa cgcactcgag agctggctga ccggcaccag gtcgtcgccc
     4261 agcagcatcg cccggtactg cggcgtaatc gtctggcccc aggtcttgtc ggcctgccgc
     4321 tcctcataaa ccgagatgcc ctcgctcagc cagcggggca tcttgttctt ggtcttgttg
     4381 agcgttacga cgtggcagaa ctcgtgccac agcgtcgcct cccagcagga cgggctcgcg
     4441 ccctgcgagg ccgggctgtt ggccgtgatc accgtgccga aacagacgcc gaggaacccc
     4501 gcgccgccgg gcatgccgaa ggtgcggatc gcaaaatcct gctgctgcgg gaacatctcg
     4561 acgacgatcg gctgcggcag ttgcacggcg tactttgcgc tcagcccctt cttggcccgc
     4621 ttcagcaggt cgagcacgcg gcggccgtag atatcggcct cgcgggcgtc catccgcagc
     4681 atcaggccgt cttcttccag cgtgcggaac ttggcgatgt tctcctgcag cgtgaccagg
     4741 ttgtgggcgt tgacgttata gccgtcggcg tcgtagacct cccccgcgag cttccagccc
     4801 tcttcctcgc ggcccaggcg gagcaagtcg gtggccagct gcgacttcgc gggaaggaag
     4861 ttgggatcga acttcagggc ctgccgctga tactgctctc cttccgcgaa gcgatacttt
     4921 tgcgagagct tcttgccgat caggtaatcg acctccgggt tggtcggcca gggcttcagc
     4981 gcggcggccc ggaaagcctt ctccttgtcg agctggttcc gcaggtgggc caggatcgcc
     5041 ttgtaagcgc cagcccgcgg atggctgggg ttgactgctg cgaccttggc cagcgtctgc
     5101 tccgccgctt cgtactgctc gccatcgatc tgttcgtcgg caatcaagag caggctgtcg
     5161 acgtggtttg gattgcgttc cagcgccgcc ttgatcgccg cttcggcccg ctccgagtcg
     5221 ctgggagcaa acgcccgggc gaggccgaag tgggctgccg gctccagcgg gtcgagcttg
     5281 acggcctgct gataggcgtc gcccgccatc aggtagtcgc ccttctcaaa ggccaggtcg
     5341 ccgattgcca ggtaaacgtc gacataggtc ggttgccgtt tccggacttc cacgtacgac
     5401 gaattgagca ccaccttggg gtcca
//
LOCUS       DASRJB010000402        14137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3213856, whole genome shotgun sequence.
ACCESSION   DASRJB010000402 DASRJB010000000
VERSION     DASRJB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213856"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VFB80_10700"
     CDS             <1..1816
                     /locus_tag="VFB80_10700"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN34282.1"
                     /translation="TIGIDTFQYQVTAGGLDSAFATVQIDVTVGDPRAVDDTRIVPFV
                     ATPVTARLPAPDLTANDISPDRPNTRITNITKVTTDPATAGAVATITADGQSIDFRPP
                     AGFRGTILYTYTIDDIDPLTSPSTAHLTIQVVDVVSGVPQPAPAHLAQFSVQFLDTTG
                     TPITMVNANDQFLIRIFSQDLRAGGAFFDRGVVAAYLDLLFNSNLADPVLSNANILGF
                     DIDWAVSASGVIGADSVQAAPAPTATSFSGSSGLPGDDGTFDRTYITFTSGALAGQRG
                     QVLSYTGATRRFNFQAGTFSAAPAAGDSFTISRPIYSIAQNGEANSPAPGAIDEVGGT
                     FLPSQTTAIFGPGKGPVPVFTVRMTAKAPGTLTVSPDPADSPANPQTTVIVTGLNPPG
                     DVSTPITDEQVFLLPSSLTILADTEGEFTNLRNPLDVSSDGKVSAIDALMVINDLNAF
                     GARSLSQLSLGMNGWLPPQFFLDVNHDTSVSPLDALMVINHLNASLASSPPQAPPGAG
                     GEGEGEGEAAPDGASFVLALTAPQADQEGGDAGESAPPESQTTSTAGSTSADTSRRGT
                     NTSDRPLARRLARLEAEAAADEVFATLSQILRSRFGRK"
     gene            complement(1861..2091)
                     /locus_tag="VFB80_10705"
     CDS             complement(1861..2091)
                     /locus_tag="VFB80_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN34283.1"
                     /translation="MPLYEYACRDCDAEFELLIRGSETPECPTCASQRLEKLLSVPAA
                     HSASSRDLPTCRPMPSGGCGLPQCGQGLCGME"
     gene            2177..2842
                     /locus_tag="VFB80_10710"
     CDS             2177..2842
                     /locus_tag="VFB80_10710"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZN34284.1"
                     /translation="MRHLTRAEVREIDRRAIVEFGLSGLVLMENAGRGCVEVLCRLGC
                     AGPVTIVCGKGNNAGDGFVIARHLDARAIPVRVVLLGAPAVLRGDAAVNYSVLSRCQV
                     PIGDLSAEFDTQAFTEQLAGAEWIVDALLGTGASGEPRENIARAIRLMNACRAKKLAV
                     DLPSGLDCDSGVPAQQTFEADHTCTFVAPKIGFAQPAAQAVLGQIHVIDIGVPRVLLA
                     AEP"
     gene            complement(2843..5488)
                     /locus_tag="VFB80_10715"
     CDS             complement(2843..5488)
                     /locus_tag="VFB80_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZN34285.1"
                     /translation="MQAVLSARRARLCALVCWGACVAFCNLGRAEQVTLANGMTLDGG
                     LGKIARLGADPLKAVGAGENLRILVVDNQLTRTFTFHDPGRLQLGASAPTPEERIEID
                     QRVQRVGRQIFSVGPILRVDPFDDYGHRIFSMSSTSGTADVVQGITIITPKWTQVEAI
                     QGPGSYVWMMRIATSTIPRQQLSKILYRQIDPKNSEQRLSIVRLYMQSERFQDARLEL
                     EQVIKDFPGLTRLQDTVKSLHQLSAQRLLKEVELRLDAGQYRLGIQMLENFPSDGVAG
                     EMLLKVRETLKEMSDLNSVGTNTLKQLDEHVAGLTNDKNRADVKPIMDEIKRDLNANT
                     LVRLADYLRLANDEKALPEQKLAFAISGWLMGSGSATENFATTLSLVRVRELVQKYLV
                     TSRQPEREAILAELASQEGATPENVSKIIAHMKPPLAASLPGAGVPDLGNPAEVLGTQ
                     APNPKLPAAPPPPMPPAAAPPADKKGPAGKAAFDPLGGCGDPPANGGVLDVQPDKPLP
                     AIPPPAADVAAPPPAPNQGWYAGVSGLLEWTTKGVPEDPQLKYYVQLPPQYDPYRRYP
                     CIVTLNGAGTTPLQQIDWWAGGYDPKADNRFGQATRHGYIVIAPRWTREHQRKYECSS
                     REHAAVLLALRDAIKRLAIDTDRVFLSGHSMGGDAAWDIGLSHPDLWAGVIPIVATTS
                     KYIAHYSENAHYVPLYFVMGEKDGDKLEKNADDLNRYVGYLNYDAMIVQYQGRGHEHF
                     LDEILNLFTWMNLHQRTFFVRDFEVDTLRPWDNFFWWVEGSRFLDSNIILPAEWGEKP
                     PRKPVAARTEGHVLPTNGVNVRSAARKVTVWLSPEIVKFEQPMTITINNKRQTKVQPS
                     TATILEDVRTRGDRQHPFWAKVETP"
     gene            complement(5629..7029)
                     /locus_tag="VFB80_10720"
     CDS             complement(5629..7029)
                     /locus_tag="VFB80_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34286.1"
                     /translation="MSADEVKRISRLARRDALRAGALSLFGLSLPQLLSARAASGAGE
                     ASFGRAKSCILLFMWGGPAHQDTWDMKPDAPAEIRGEFAPISTNVPGIQICEHFPQLA
                     RRCDQLALVRSVTHNNADHTPSTHFLLTGQPPPTSKDVRVQWPSLGAVLSRLGRGRSA
                     LPPFVSMRPKVEGDVPRFVEESQGQFAGWIGAAHDPLTIDDDPSRADFRVPGLTLNDG
                     LNQQRLQQRLALRAGFNERLPAGTAAATAQEQNLQRAYDLLTSAADQRGAFDLAQEPA
                     KVRERYGLNPHGQSVLQARRLVERGVPLVTVFWPNDGIKNVSVYWDTHSRNFIDLKQR
                     LMPVADQAFSALLDDLSERGMLDETLVVWTGEFGRTPRVGQRNSDAGAGRDGRDHWPG
                     CFTSVLAGGGIRGGLVYGKSDKHAAYPSADPVAPADLVATIYHTLGVPEQQTLPDTSG
                     RPIFVRTGKLLPELLA"
     gene            7162..7401
                     /locus_tag="VFB80_10725"
     CDS             7162..7401
                     /locus_tag="VFB80_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34287.1"
                     /translation="MIPRWSLKTLVFALPVLVVAFGVLMGASQLAAALEDAGGARLLL
                     RLAIAGLILLAIDAVLLLAVLGLRAIDADERGEGE"
     gene            7471..8535
                     /locus_tag="VFB80_10730"
     CDS             7471..8535
                     /locus_tag="VFB80_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34288.1"
                     /translation="MPIRFACPQCRQKLSISSRRAGQTATCPRCRSTLTIPTPPPEPE
                     RPMASAPVLPPFPAAVVQEQEERQVAQPPPAPQPAAAQQEQPPADAIAAGVQTPGPLP
                     EPPPEPPSAVLPKEEAAAPPPEPTAFEPNFAPDVGSGFEGLELVYDSPAPEERSEPPP
                     VASDLIAVPRYAIYLQGGLLALVAFLAFVIGILAGGTLLSGPAAPAAPQACVISGSIT
                     YVSGPRQLPDEGAVIAVIPQSQNRPDEKVPVVGLRPGDPTPDADHRGIAILREFGGGY
                     TRADAGGRFQLQVPSQGRYLLLVISHEKPAGSEGPANSADIVKLGPYFANAADLLGDR
                     RYQLTQETLRGDKQLSVAFQ"
     gene            complement(8536..9108)
                     /locus_tag="VFB80_10735"
     CDS             complement(8536..9108)
                     /locus_tag="VFB80_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34289.1"
                     /translation="MQRQLTAFVDQPDKATFLSARDAVLRLSPLPIKATDLADLERLL
                     ERDEAQAVLDRLDTLPPSKVLSPRVHYLAAEAAALLGDSQTVELERSLFVLTLQGLLA
                     TGDGSRGNPYVVCHSTDEYDVLESLGHEPAGQKLVEHEGRLHDVLLCSDGRKVWFDIT
                     DILVRPQPRKRPAGRSRRSARRRQISRVHR"
     gene            9422..9646
                     /locus_tag="VFB80_10740"
     CDS             9422..9646
                     /locus_tag="VFB80_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34290.1"
                     /translation="MQFNLFDWIREGVRRSVIQGVSDAMDSIGNPEGEDGRARLAHLL
                     SVPAEAEPALAGAKRKRLGRSLRDFEGAAE"
     gene            9650..12202
                     /gene="hrpB"
                     /locus_tag="VFB80_10745"
     CDS             9650..12202
                     /gene="hrpB"
                     /locus_tag="VFB80_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HZN34291.1"
                     /translation="MPTGNRVIQYEGVQKLSPLPIDDILGDVTARLRAAPSLVLCAPP
                     GAGKTTRVPPALLAAGLAEAGRILVLQPRRVAARATAARIAAERSWRLGEEVGYQVRF
                     ESRVGPGTKIAIITEGILLRRLLDDPFLQGVGAVVFDEFHERSLASDLALAMVRQVQQ
                     TVRPELRIVVMSATLAAEPIAEYLGGCPIVESQGRIHPVEVRYLPHLSRQPLVELVAE
                     GVASVLDQSPGDVLVFLPGVGEIRRCAERLAPLAERRDLRLLQLYGDLPAEQQDAVLQ
                     PSNRRKIILSTNVAETSLTIEGVTAVVDSGLARQLRFDERTGLDHLELAPISQAAAAQ
                     RAGRAGRTAPGICLRLWPEAAHRSRPEYESPEIARVDLAGAVLQILCWIEPDLAAFGW
                     FDPPPAGAIDRARRLLELLGAVTGNEITDLGRRIAALPAHPRIGRLLLEGQRRGVLAE
                     ASLAAALLSERDPFVRQPGQRRAASTKSRSDVLDRVHALEQFEKSGQADSELGALNRG
                     AAKNILRTRDQLLRLLPGEPGNAGDEAFFRSLLAAYPDRLARRRELDSRRALMVGGRG
                     VRLADEVAIADEELFLCIDVNGSGGEGLVRQASAVERAWLPAELVRIETVVEFDEASE
                     RVAARRRTTFEGLLLEEAQAALPGGEVVARVLAEAAAVRLERAFPADDSAVAEFRTRV
                     RCLAEWMPELRLPQLDDETVRGLLPQLAAGRRSLAELRAAPWLHALKSLFDWQQLQAI
                     DREAPERIEVPSGSRIAVQYEAGRPPILAVRIQEVFGLLETPRIAGGRVSVLMHLLAP
                     NMRVAQVTGDLASFWANTYAMVRKDLRARYPKHAWPEDPYTARAEKRPGRKR"
     gene            complement(12258..12740)
                     /locus_tag="VFB80_10750"
     CDS             complement(12258..12740)
                     /locus_tag="VFB80_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34292.1"
                     /translation="MSPIITTLSALVVSLGIAPRENVAREAVDLIEVNHFYDEHGRLV
                     FDQVIFYDWSQDDARYMVRAWRLVKNPAQLPQRDWKDGGYAAVWQDGDLMRHVRAKSF
                     RETWTQYDPELIEREFLPKERRKELMTVKVNRQPQAVAAQPAPQPRAVPSSASTAVNP
                     "
     gene            13084..14106
                     /locus_tag="VFB80_10755"
     CDS             13084..14106
                     /locus_tag="VFB80_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN34293.1"
                     /translation="MVNSRRLDSPFRNVSLGAGGADSEGPTGPPEVRRDADLLDAYSQ
                     AVIGVVSRIGPAVLSITGHPGDGQPGQGSGFLITPDGFALTNSHVARARPRLRAITEE
                     GDSLEARLIGDDPATDLALVRLAARDLPHAELGDSGGLQVGQLVIAMGNPFGFRSTVS
                     TGVVSARGRAMRSEQGRLIENIVQHTAPLNPGNSGGPLVDSRGRVVGINTAIIAMAQG
                     LGFAVPATTARWVVGELLTHGRVRRLSLGITVTVAPIPRRLARELDLLADQAVEIVEV
                     AARGPAAAAGLRPGDWLVSAGGRIVSSTDDLHRVLTSIKDARGIALQVVRDERLLEIA
                     VTPAWQ"
BASE COUNT         2206 a         4713 c         4846 g         2372 t
ORIGIN      
        1 gacgatcggc atcgacacgt tccagtatca ggtgaccgcc ggcggcctcg attcggcgtt
       61 tgcgaccgtg cagatcgacg tgacggtggg cgaccccagg gcggtcgatg acacgcggat
      121 cgtgccgttc gtcgccaccc cggtgacggc ccgcctgccg gccccggacc tgacggcgaa
      181 cgacatttcg cccgataggc ccaacacgcg gatcaccaac atcaccaagg tcacgaccga
      241 tccggcgacg gccggggcgg tggccacgat cacggccgac ggccagtcga tcgacttccg
      301 gccccccgcg ggcttccgcg gcacgatcct ctacacctac acgatcgacg acatcgaccc
      361 tttgacgagc ccgtccacgg cccacctcac gatccaggtg gtcgatgtgg tcagcggcgt
      421 gccgcagcct gcgcccgccc acctggcgca gttcagcgtg cagttcctgg atacgacggg
      481 cacgccgatc acgatggtca acgccaacga ccagttcctg atccggatct tctcgcagga
      541 tctgcgggcc ggcggcgcgt tcttcgatcg cggcgtcgtg gccgcctacc tggacctgct
      601 gttcaactcg aacctggccg atccggtgct cagcaacgcc aacatcctcg gcttcgacat
      661 cgattgggcc gtgagcgcaa gcggcgtgat cggcgcggat tcggtgcagg ccgcgccagc
      721 cccgaccgcc acgagcttct ccggcagctc cggcctgccc ggcgacgacg gcacgttcga
      781 ccgcacctac atcacgttca cctcgggcgc tctggccggc cagcgcgggc aggtgctcag
      841 ctacaccggc gctactcgcc gcttcaactt ccaggcgggc acattctccg ccgcccccgc
      901 cgccggcgac tcgttcacca tcagccggcc gatctacagc atcgcccaga acggcgaagc
      961 caattcgccg gcgccgggcg cgatcgacga ggtcggcggc acgttcctgc ccagccagac
     1021 cacggccatc ttcggccccg gcaagggacc ggtgcccgtg ttcaccgtgc ggatgacggc
     1081 caaggccccc ggcacgctga cggtcagccc cgacccggcc gattcacccg cgaacccgca
     1141 gacgacggtc attgtcaccg gcctcaatcc gccgggcgac gtctcgacgc cgatcaccga
     1201 cgagcaggtg ttcctgctgc cctcgtcgct gaccattctg gccgacacgg agggcgagtt
     1261 caccaacctc cgcaacccgc tggacgtcag cagcgacggc aaggtctcgg ccatcgacgc
     1321 cctgatggtc atcaacgacc tcaacgcctt cggcgcgcgg tcgctgtcgc agctcagcct
     1381 gggaatgaac ggctggctgc cgccgcagtt cttcctcgac gtcaaccacg acacgtcggt
     1441 ctcgccgctc gatgcgctga tggtcatcaa ccatctcaac gcctcgctgg cctcgtcgcc
     1501 cccgcaggcg ccgccgggcg ctggcggtga aggtgagggc gagggcgaag ccgcgccgga
     1561 cggcgcgtcc ttcgtgctgg ccctgaccgc gccgcaggcc gatcaggagg gcggcgacgc
     1621 aggcgaatct gctccgcccg agtcgcagac gacgtccact gccggatcga cgtcggccga
     1681 cacgtcccgc cgcggaacca acaccagcga tcgcccgctg gcccgccggc tggccaggct
     1741 cgaagccgag gctgcggccg acgaggtgtt tgccacgctg agccagatcc tccgcagccg
     1801 cttcgggcgc aagtagcggg cggtccctta gccccggcgt ccacgccggg cgggtggccg
     1861 ctactccatc ccgcacaggc cctgcccgca ctggggcagg ccgcagccgc cgctgggcat
     1921 cggccggcag gtcggcaagt cgcggctgct ggcactgtgc gccgccggca cgctgagcag
     1981 cttctccaag cgctggctcg cgcaggtcgg acactcgggc gtctcgctgc cgcggatcaa
     2041 gagctcgaat tcggcgtcgc agtcgcggca ggcgtattcg tagaggggca tggcggggaa
     2101 atgtcgaatg gcgaaaatca attgaggaaa gaacgtcgaa taccattttg atccaacagt
     2161 cctttcccgc aaagccatgc gccacctaac gcgcgccgaa gtccgcgaaa tcgaccgccg
     2221 ggcgatcgtg gaattcggcc tgagcgggct ggtgctgatg gaaaacgccg gccgcggctg
     2281 cgtggaggtg ctttgccggc tgggctgcgc cgggccggtg acgatcgtct gcggcaaggg
     2341 gaacaacgcg ggggacgggt tcgtgatcgc ccggcacctg gacgcccgcg cgattccggt
     2401 gcgggtcgtg ctactgggtg cgccggccgt actgcggggt gacgcggcgg tcaattattc
     2461 ggtgctcagc cgctgccagg tcccgatcgg cgacttgtcg gcggagttcg acacccaagc
     2521 cttcaccgaa cagctggcag gcgcggaatg gatcgtcgat gccctgctgg ggaccggcgc
     2581 gagcggagag ccccgcgaga atatcgcccg ggcgatccgt ctgatgaatg cctgccgggc
     2641 gaagaaactg gcggtcgatc tgcccagcgg gctggactgc gacagcggcg tgcccgcgca
     2701 gcagactttt gaggccgacc acacctgcac gttcgtcgcc cccaagatcg gcttcgctca
     2761 gccggcggcc caggcggtgc tgggccagat ccacgtcatc gacatcggcg tgccgcgcgt
     2821 gctcttggcg gctgagccgt agctacggcg tttcgacctt cgcccagaac gggtgctggc
     2881 ggtcgccgcg ggtgcggacg tcctcgagga tcgtggcggt gctcggctgg accttcgtct
     2941 gccgcttgtt gttgatcgtg atcgtcatcg gctgttcgaa tttgacgatc tccggcgaca
     3001 gccaaaccgt gaccttgcgt gcggcggacc tcacattgac gccgttggtg ggcagcacat
     3061 gaccttcggt gcgtgcggcg accggtttgc ggggcggctt ctcgccccat tcggccggca
     3121 gaatgatgtt ggaatcgaga aacctgctgc cctccaccca ccagaagaaa ttgtcccacg
     3181 ggcggagcgt atcgacttcg aagtcgcgga cgaaaaatgt ccgctggtgg aggttcatcc
     3241 aggtgaacag gttcaggatt tcatccaaaa agtgctcgtg tccgcgtccc tggtactgca
     3301 caatcatcgc gtcgtagttg aggtaaccga catagcgatt caggtcgtcg gcgttctttt
     3361 ccagcttgtc gccgtctttc tcgcccatga cgaaatagag gggaacgtag tgggcgtttt
     3421 cggagtagtg tgcgatgtat ttgctggtgg tggccacgat cgggatcacg ccggcccaca
     3481 gatcgggatg ggacaggcca atgtcccagg ctgcgtcgcc gcccatcgaa tgcccgctga
     3541 ggaacacgcg gtcggtgtcg attgccagcc gcttgatcgc gtcgcggagc gccagcagca
     3601 ccgccgcatg ctcgcgcgag gagcattcgt atttccgctg gtgctcgcgg gtccagcggg
     3661 gagcgatgac gatgtagccg tgccgcgtgg cctggccgaa gcggttgtca gccttggggt
     3721 cgtagccgcc ggcccaccag tcgatctgct gcagcggcgt cgtaccggcc ccgttgagcg
     3781 tcacgatgca gggataccgc cggtagggat cgtactgcgg cggcaattgc acgtagtact
     3841 tcagctgcgg atcctcgggc acgcccttgg tggtccactc caagaggccg ctgacgccag
     3901 cgtaccagcc ctggttagga gcgggcggcg gggcggcgac atcggccgcc ggcggcggaa
     3961 tcgccggcag cggcttatcg ggctgaacat ccagcacgcc gccgttggcc ggcggatcgc
     4021 cgcagccccc gagcggatcg aaggccgcct tgccggccgg ccccttctta tccgcgggcg
     4081 gagcggcagc gggcggcatt ggcggcggag gcgccgccgg caacttggga ttgggggcct
     4141 gtgtgcccag cacttcggcc gggttgccca ggtcgggaac gcccgcgccg ggcaacgacg
     4201 ccgccagcgg cggcttcatg tgggcgatga ttttggagac gttctcgggc gtggcccctt
     4261 cctgtgaggc caactccgcg aggatcgcct cccgctccgg ctgccgcgag gtaaccaggt
     4321 atttctggac caactcgcgg acgcggacca gcgaaagcgt cgtggcgaaa ttctcggtcg
     4381 ccgagccact gcccatcagc caaccgctga tcgcgaaggc cagcttttgc tcgggcagcg
     4441 ccttctcgtc attggccagc cgcaggtagt ccgccaggcg aacgagcgta ttggcgttca
     4501 gatcgcgttt gatctcgtcc atgatcggct tgacgtcggc gcggttcttg tcgttggtca
     4561 agcccgcgac gtgctcgtcg agctgcttca gcgtgttcgt gccgacggag ttcaggtcgg
     4621 acatctcctt gagcgtctcg cggactttga gcagcatctc gccggccacg ccgtcgctgg
     4681 gaaaattctc cagcatctgg atgcccaggc ggtactgccc cgcgtcgagc cgcagctcga
     4741 cctccttgag cagccgctgg gccgagagct ggtgcagcga cttgaccgtg tcctggaggc
     4801 gggtcaggcc cgggaaatcc ttgatgactt gctccagctc cagccgggcg tcctgaaagc
     4861 gctccgactg catatacagc cgcacgatgc tcagtcgctg ctcggaattc ttggggtcga
     4921 tctgacgata gaggatcttg ctgagttgct ggcgcgggat ggtgctggtg gcgatccgca
     4981 tcatccagac gtacgagccg ggcccttgga tcgcttcgac ctgcgtccac ttcggggtga
     5041 tgatcgtgat tccctgcacc acgtcggcgg tgccgctggt cgagctcatc gagaagatgc
     5101 ggtggccgta atcgtcgaac ggatcgaccc ggaggatcgg gcccacactg aagatctgcc
     5161 ggccgacgcg ctgcacgcgc tggtcgatct cgatccgctc ctcgggcgtc ggggcgctag
     5221 cgcccagctg gagccgcccc gggtcgtgaa aggtgaaggt gcgcgtgagc tggttatcga
     5281 cgacgaggat gcggaggttt tcgccagcgc cgaccgcctt gagcggatcg gctcccaggc
     5341 gggcgatctt gcccaggccg ccgtcgagcg tcattccgtt ggccagcgtc acctgttccg
     5401 cccggccaag attgcaaaag gctacgcaag cgccccagca caccaacgcg cacagcctgg
     5461 cccgccgtgc cgacagaacc gcctgcatga gaagctcctt cgcgctctgt tccgcggctg
     5521 cccccaagcc gtacgctcca ttgtcgtgcg gccggcggca atgtcaacgc aagcactgta
     5581 gtccgctcgc tctgcgagcg gacagcctca cgcggagcgt gaggactact aggccagcaa
     5641 ctccggcagg agcttgcccg tacgcacgaa gatcggccgg ccgctggtgt cgggaagcgt
     5701 ttgctgctcg ggcacgccga gggtgtgata gatggtggcg acgaggtcgg ccggcgcgac
     5761 gggatcggcg gacggatagg ccgcgtgctt gtcactcttg ccatagacca ggccgccgcg
     5821 gattccgccg ccggcgagca cgctcgtgaa acagccgggc caatggtcgc ggccgtcgcg
     5881 gcccgccccg gcgtcgctgt ttcgctggcc gacgcgcggc gtgcggccaa actcgcccgt
     5941 ccagacgacg agcgtctcat ccagcatccc gcgctcgctc aggtcgtcga gcaaggccga
     6001 gaacgcctgg tcggcgaccg gcatcagccg ctgcttgagg tcgatgaagt tgcggctgtg
     6061 cgtgtcccaa tagacgctga cgttcttgat gccgtcgttc ggccaaaata cggtcaccag
     6121 cggcacgccg cgctcgacca gccgccgagc ctggagcacg gattggccgt gcgggttgag
     6181 accgtaccgc tcgcggactt tcgcgggctc ctgggccagg tcgaaggccc cgcgctgatc
     6241 ggcggcggag gtgagcaggt cgtaggcccg ctggagattc tgctcctggg ccgtcgcggc
     6301 cgccgtgccg gccggcagcc gctcgttgaa tcccgcccgc agcgcaagcc gctgctgcag
     6361 gcgctgctga ttgagcccat cgttcaaggt cagcccgggc acccgaaagt cggcccggct
     6421 ggggtcgtcg tcgatcgtga gcggatcgtg ggccgcaccg atccagcccg cgaactgccc
     6481 ctgactttct tcgacgaacc gcggcacgtc cccttcgacc ttgggccgca tcgaaacgaa
     6541 gggcggcagg gccgagcgac cgcggcccaa tcgagagagg accgcgccaa ggctcggcca
     6601 ctgaacgcgg acgtccttgc tggtcggcgg cggctggccg gtgagcagga agtgcgtgct
     6661 gggcgtgtgg tcggcgttgt tgtgcgtcac cgagcggacc agcgcgagct ggtcgcagcg
     6721 gcgggccagc tgcggaaaat gctcgcagat ttgaatgccc gggacgttgg tcgagatcgg
     6781 cgcgaattcg ccgcgaatct cggctggcgc gtcgggcttc atatcccagg tgtcctggtg
     6841 cgccggtccg ccccacatga atagcaggat gcacgacttc gcccggccga aggatgcctc
     6901 gccagcgccc gatgctgcgc gagccgacag aagctgtggc agcgagagcc cgaatagact
     6961 cagtgccccc gcacgcaagg cgtcacgccg cgcgagccgg ctgatccgct tgacttcgtc
     7021 ggcagacatg gaggcgattc tctccgcagg cgctgtcgcc tccggctcgg ggtttaacga
     7081 aattagatta ccaccgcgaa gccgcgctcg aaacgtcctt tctgtgttaa tatcagcaac
     7141 tccgcagcaa gagccactcc tttgattccc cgctggtccc tgaaaacgct cgtctttgcc
     7201 ctgccggtgc tggtggtggc gtttggcgtc ttgatggggg cgtcgcagct ggcggcggcc
     7261 ctggaggatg ccggcggcgc tcggctgctg ctgcggttgg cgatcgcggg gctgatcctg
     7321 ctggcgatcg atgctgtgct gctgttggcg gtgctgggac tgcgggcgat cgacgccgac
     7381 gagcgcggcg agggggaata gaaactgggc acgcccgcgg ggcggtgtta gtgtaagagg
     7441 gtcgcccctt cgcgtgcgat ttgcccgcct atgccgatcc gtttcgcctg cccgcagtgc
     7501 cgccagaagc tgagcatcag ctcgcgccgg gcggggcaaa cggcgacctg ccctcggtgc
     7561 cgcagcacgc tcacgatccc aacgccgccg ccggagccgg agcggccaat ggcgtccgcg
     7621 cccgttcttc cacccttccc agccgcggtg gtgcaggagc aggaagagcg gcaggttgcc
     7681 cagccgccac cggcgccgca gccagccgcg gcccaacagg agcagccgcc ggcggatgca
     7741 atcgcggcgg gcgtccagac gccaggcccg ctgcctgaac cgccacccga accaccgtcc
     7801 gcagtgctcc ccaaggaaga ggccgcagcg ccaccgccgg agcccacggc cttcgagccc
     7861 aacttcgcgc cggacgtcgg cagcgggttc gaggggctgg agctggtcta cgattctccc
     7921 gcgccggagg aaaggagcga gccgccgccg gtggcgtccg atctgatcgc ggtgccgcgg
     7981 tatgcgatct acctgcaggg cggcttgctg gcgctggtcg cgtttctggc attcgtgatc
     8041 ggcatcctcg ccgggggaac gctgctcagt ggcccggccg cgccggccgc gccgcaggcc
     8101 tgtgtcatca gcggcagcat cacgtacgtt tccggcccgc ggcagctgcc cgatgagggc
     8161 gcggtcatcg cggtcattcc ccagtcgcaa aatcgccccg acgagaaggt gcccgtcgtc
     8221 ggcctgcgcc ccggcgatcc tacgcccgac gcggaccacc gcggcatcgc gatcctccgc
     8281 gagtttggcg gcggctacac gcgggccgac gcgggcgggc ggtttcagct gcaggtgccc
     8341 agccagggcc gctatctgct gctcgtcatt tcgcacgaga agccggcggg ctcggaaggg
     8401 cccgccaact cggccgacat cgtcaagctc ggcccctatt tcgccaacgc cgccgacttg
     8461 ctcggggacc gccgctatca gctcacgcag gagacactcc gcggcgacaa gcagctcagc
     8521 gtggcgtttc agtagctagc ggtgcacgcg gctgatttgc cgccgccggg ccgagcggcg
     8581 cgaacgcccc gcgggccgtt ttcgcggttg cggccgcacg aggatgtccg tgatgtcgaa
     8641 ccagaccttg cggccgtcgc tgcagagcag cacgtcgtgc aagcggccct cgtgctcgac
     8701 cagcttctgg ccggccggct cgtgtccgag cgactccagg acgtcgtatt cgtccgtgga
     8761 atggcagacc acgtaggggt tgccgcggct gccgtcgccg gtggccaaga ggccctggag
     8821 cgtgagcacg aacagcgagc gttccagctc aacggtctgg gaatcgccga gcagcgcggc
     8881 cgcctcggca gccagataat ggacgcgggg cgaaaggacc ttcgacggcg gcagcgtgtc
     8941 gagccggtcc aggacggctt gcgcctcatc gcgttcgagt agacgttcca gatcggccag
     9001 gtcggtcgcc ttgatcggca gcggcgagag ccggaggacg gcgtcacggg ctgagaggaa
     9061 agtcgccttg tcgggctggt ccacaaacgc ggtgagttgg cgctgcatga gaaggctctc
     9121 ggagaccgca ggagcggcgc tgctcgcggc gtttccgtcg ccgcgcgcag ccaagtgcgc
     9181 cgcgctacat tgcgcttagc gtaatcaaca cgcgcgctcg ttggcaatga cttggcgcgc
     9241 gggaagagaa aagtttttta gcggtgtcat tggtcattgt cattgcgcag tggcgaataa
     9301 caaacgacag gtgaccaatg acaaatgaca aacgaccaga gtcgctcaag ttccgtggcg
     9361 ctcaccgccg aagtaagacg aggcggcgga gtccgccttt cccacggcaa ggagtgctgg
     9421 catgcagttc aatctgttcg actggattcg tgaaggcgta cgccgcagcg tgattcaggg
     9481 cgtttcggat gcgatggatt cgattggcaa ccccgaaggc gaagacggcc gcgcgcggct
     9541 cgcgcatctg ctgagcgtgc ccgcggaggc cgagcccgcc ctggccggcg cgaagcgcaa
     9601 gcggctgggc cgctcgctcc gcgacttcga gggcgccgcg gaatagactt tgccgaccgg
     9661 caaccgcgtg attcaatacg agggcgtgca gaagctcagc cctcttccga tcgatgatat
     9721 cctcggcgat gtgacggcgc ggcttcgcgc tgcgccgtcg ctcgtgctgt gtgcgccacc
     9781 cggggcgggc aagaccaccc gggtgccgcc ggcgctgctg gcggccggcc tggcggaggc
     9841 gggccgcatc ctcgtccttc agccgcggcg cgtcgccgcc cgggccaccg ccgcccggat
     9901 cgccgccgaa cgctcctggc gactgggcga ggaagtcggc taccaggtcc gcttcgagtc
     9961 gcgggttggg cccggcacga aaatcgccat cattaccgag ggaattctgc tccgccggct
    10021 gctggacgac cccttcttac agggcgtggg cgctgtcgtt ttcgacgaat tccacgagcg
    10081 ctcgctggcc agcgacctgg ccctggcgat ggtccggcaa gtccagcaga cagtccgccc
    10141 ggaactgcgg atcgtcgtca tgtcggccac gctcgccgcc gagccgatcg cggagtatct
    10201 cggcggctgc ccgatcgtcg agagccaggg gcgcatccat ccggtcgaag ttcgttacct
    10261 gccgcatctg tcgcggcagc cgctcgtcga gttagtcgcc gaaggcgttg cttccgttct
    10321 cgaccagtcg ccgggcgacg tgctggtgtt cctgcctggc gtgggcgaaa tccgccgctg
    10381 cgccgagcga ctggcgccgc tggccgagcg gcgcgatctg cgcctgctcc aactctatgg
    10441 cgatctgccg gctgagcagc aggacgcggt gctgcagccg agcaaccggc ggaagatcat
    10501 cctctcgacg aacgtcgccg agacttcgct gacgatcgag ggcgtcacgg ccgtcgtcga
    10561 tagcggcctg gcacggcagc tgcgcttcga cgagcggacg gggctcgatc acttggagct
    10621 cgcgccgatt tcgcaagccg cggcggcgca gcgggcgggc cgcgcgggac gcaccgcgcc
    10681 gggaatctgc ctgcggcttt ggcccgaggc ggcacaccgc agccgcccgg agtacgagtc
    10741 gcccgaaatt gcccgcgttg atctggcggg cgcagtgctg cagatcctgt gctggatcga
    10801 gccggacctg gcggccttcg gctggttcga cccgccgccg gccggcgcga tcgatcgcgc
    10861 gcggcgcctg ctggagctac tcggcgcagt caccggcaac gagatcaccg acctcggccg
    10921 ccgtatcgcc gcgctgcccg ctcacccgcg gatcgggcgc ctcttgctcg aaggccagcg
    10981 ccgcggcgtg ctcgcggagg cttcgctcgc tgcggcactg ctgtccgagc gcgatccctt
    11041 cgtccggcag ccgggccagc ggcgcgcggc gagcaccaag agccgtagcg acgtgctcga
    11101 ccgcgtgcat gcacttgagc agttcgagaa atcaggccag gccgacagcg aactcggcgc
    11161 gctcaaccga ggcgcggcca aaaacatctt gcggacgcgc gaccagctgc tgcggctgct
    11221 gcccggtgag ccaggcaacg ccggcgacga ggctttcttc cggtcgctgc tggcagccta
    11281 tcccgatcgg ctggcccgcc gccgtgaact ggacagccgg cgggccctga tggtcggcgg
    11341 ccgcggtgtg cggttggccg acgaggtggc catagccgat gaggagctct ttttgtgcat
    11401 cgacgtcaat ggcagcggtg gcgaagggct cgtgcggcag gcttcggcgg tcgaacgagc
    11461 gtggctgccc gcggagctcg tacggatcga gacggtcgtc gagttcgacg aggccagcga
    11521 gcgcgtcgcg gcccgccgga ggacgacctt cgaggggctg ctccttgagg aagcgcaggc
    11581 ggcattgccc ggcggcgaag tggtcgcccg cgttctggcg gaggcggccg ccgtgaggtt
    11641 ggagcgggcg tttccggccg acgactcggc cgtcgccgag tttcgcactc gcgtacggtg
    11701 cctggccgag tggatgcccg agctcaggct gccgcagctc gatgacgaga cggtgcgcgg
    11761 actattgccg cagctcgccg cagggcggcg atcgctagcg gagttgcgtg ccgccccttg
    11821 gctgcatgca ctcaaaagcc tgttcgactg gcagcaattg caagcgatcg accgcgaagc
    11881 gccggagcgg atcgaagtgc ccagcggcag ccgcattgcc gttcagtacg aagccggccg
    11941 gcctccgata ctggcggtgc gcatccagga agtgttcggt ctgctcgaaa cgccgcggat
    12001 cgccggcggc cgtgtgagcg tgctgatgca cctgctcgcg ccgaacatgc gtgtggccca
    12061 ggtcaccggc gacctggcca gcttctgggc caatacgtac gcaatggtcc gcaaagactt
    12121 gcgggcccgc tatcccaagc acgcctggcc ggaagacccg tacactgccc gggccgaaaa
    12181 gaggcccggg cggaaacgct aagtggggca gacattcctg tctgccgctt gggatggcag
    12241 atatctgccc cacttggcta cggattgacc gcggtgctcg cagagctggg gacggctctg
    12301 ggctgtggcg ccggctgggc ggcgactgct tgcggctgcc ggttgacctt caccgtcatc
    12361 aactccttgc ggcgttcctt gggcaggaat tcgcgctcga tgagctccgg gtcgtattgc
    12421 gtccaggtct cgcggaacga cttggcccgc acgtgccgca tcaggtcgcc gtcctgccag
    12481 acggccgcgt agccgccgtc cttccagtcg cgctgcggga gctgggcggg gtttttgacc
    12541 agccgccagg cccggaccat gtagcgggcg tcgtcttgcg accagtcgta gaagatgacc
    12601 tggtcgaaaa cgaggcgtcc gtgctcgtcg taaaagtgat tgacctcgat gagatcgacc
    12661 gcttcgcggg cgacattttc cctgggcgca atgcccagag aaacaacgag ggcgctgagc
    12721 gtagtaataa tgggactcac cagtcacctc catgctgcag gtgaggactt cgcggtgaga
    12781 ccccggggtt taacgcgttg tcgtctgtta tcggaagtcc gctgcagcgt caagagaaaa
    12841 tccggcacct cgctttggaa aaatcggtga atcgttcgat tctgaacaat tctacctcgc
    12901 aaagctcgtg ccgcctgtag ttcgaagcgc aactttagga attctgtgac gcttcattcg
    12961 tcacaaatga tttattgtgg tggcaatatt tcccttaacc gcattaattg ccaactggcg
    13021 ttgcctttgc gggaacgtcg ctagaattcg acagccgcat tcttaagcag cgaggtatta
    13081 gaaatggtca actcccggcg actggacagc ccgttccgta atgtttccct tggcgcgggt
    13141 ggggccgatt cggagggccc tacggggccg ccggaggtcc gccgggacgc ggatttgctg
    13201 gacgcctatt cccaggccgt gattggcgtc gtgagtcgga ttggcccggc cgtcctctcg
    13261 attaccgggc atcccggcga cggccagccc ggacagggtt cgggcttttt gattacgccc
    13321 gacgggtttg ccctgacgaa ctcgcacgtg gcccgcgcgc ggccgcggct gcgggcgatc
    13381 accgaggaag gcgactcgct cgaagcgcgc ttaatcgggg atgatcccgc gacggacctg
    13441 gccctggtgc ggctggcggc ccgcgacctg cctcatgcgg aactgggcga ctctggcggc
    13501 ctgcaagtcg ggcagctcgt gatcgcgatg ggcaacccgt ttggctttcg ctcgacggtc
    13561 tcgaccggcg tcgtcagcgc tcgcggccgc gcgatgcgga gcgagcaggg tcggctgatc
    13621 gagaacatcg tgcagcacac cgcgccgctg aacccgggca acagcggcgg gccgctcgtc
    13681 gattcccgcg gccgggtcgt cggcatcaac acggcgatca tcgcgatggc ccaggggctg
    13741 ggcttcgccg ttcccgcgac aaccgcccgc tgggttgtcg gggaactgct gacgcatggc
    13801 cgcgtccgcc ggctgtcgct cggcatcacc gtcacggtcg cgccgattcc gcgcaggctg
    13861 gcccgggagc tggacctgct ggccgaccag gccgtggaaa tcgtcgaagt cgccgcccgc
    13921 ggtccggcag ccgcggcggg gctgcgtccg ggtgactggc ttgtctcggc cggcggacga
    13981 attgtctcca gcactgacga cttgcaccgt gtgctgacca gcatcaagga cgcgcgcggg
    14041 atcgcactgc aggtcgtccg tgatgagcgg ctgctggaga ttgccgtcac accggcgtgg
    14101 cagtagcctt gtcgccatgc tgaacgagca cgagatc
//
LOCUS       DASRJB010000403         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4061600, whole genome shotgun sequence.
ACCESSION   DASRJB010000403 DASRJB010000000
VERSION     DASRJB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4061600"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFB80_10760"
     CDS             complement(<1..261)
                     /locus_tag="VFB80_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34294.1"
                     /translation="MTDSKELLKKYEPAKDVKTITADPQVRLARYSPCGKVLAAGGYT
                     GNVRRWNVEAEPAELPALGGHHAWIDGLAFRAEGELLFTADSW"
     gene            complement(258..1532)
                     /locus_tag="VFB80_10765"
     CDS             complement(258..1532)
                     /locus_tag="VFB80_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34295.1"
                     /translation="MKCSHLCTPREHALSRRQILGAAGALGLGSLLRTAVADELKQKR
                     KQVLFIWIDGGMSQLESWDPKPNTTYGGPFRAIPTSVPGVHVSELLPRSAKIMDKLAV
                     IRSVHTQDNSHSAGVARIQRGDPKNRGVTYPYFGSAVAKLLGPGDSGLPPYVWIKPGS
                     GGFIHGDAGFIGPKYGAVAFGDGKPPENLVAPPEVPPHDADERAVLQAAANARYAKGR
                     RPEMGEATSYVYDVARTLMKRQDLFDSTRFDSKDVERYGTHEIGRHMLQARKMLEAGV
                     TFVKVNSYGWDTHGDNFNGHLSLMPKFDQAFASIVSDLDDRGLLENVLVIAMSEFGRT
                     PRINGHIGRDHWPEAWSIAMAGCGLARGAVVGETNEQGTYVKGDEHDIGSLFHTWFAC
                     LGIDTKQVQYDNGGQPLPLAHEDMKPVKELLA"
     gene            complement(1600..3195)
                     /locus_tag="VFB80_10770"
     CDS             complement(1600..3195)
                     /locus_tag="VFB80_10770"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34296.1"
                     /translation="MALPRLLAICLALPWAASAWGDDAAALHARIDALIEAKAGGPLA
                     EKASDAEFIRRIYLDLAGRLPSVEETRAFLADPAADKREKLIDKLLASGDHVRRMTQV
                     FHVMLMERLGDHAEWQKFLKASFEANKPWDQMVRDILSPDSESETARGAALWYTKRLE
                     HYGENPVDIPGMVRDVGRHFLGIDVQCAQCHDHLFVEDYKQEFYHGMLAFVGNTTIRT
                     DVQFPAVALKPLDKKVEFMSVFLQKPLAVGPKLPLGQEIEIPTFGKGEEFEQPPDKKT
                     KFPGKLKFDTLKLLGEQLPRGENQLFTKNIANRLWWLMLGRGLVHPLDLSHKANPASH
                     PELLELLASELAAHQFDMRWLIREIALSQTYQRSGVLAGEVLPQSYRAALEKPLSSEQ
                     MLASIQQALGDGQPLAIDPAAKTWTDWQTRFDKALANPAREPEVGHNPTVKAALFLMH
                     DTTLLAWLKPSGDSLTARLLKESDAAKLADELYLAVLSRSPTAEEAAAVGEYLTPRAD
                     HRESAVSNLIWSLIASNEFCTNH"
     gene            complement(3472..5571)
                     /locus_tag="VFB80_10775"
     CDS             complement(3472..5571)
                     /locus_tag="VFB80_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34297.1"
                     /translation="MPHRLFVAALLAAATSLSTAGRGADAIPPIPRVLPPEGLEIPAE
                     VRTRLERQLAETKQRLKKFESDVEGLQDEELKEEVLTGKVSPHNRLWRYPLDVEVFVK
                     AVEFALLHREFYVPKDFEKADWALDQANQRLDAMAKDEVPWSRATGLVVRGYRSKIDG
                     SVQPYGVVIPPDHDFSKPCRVYVWLHGRGDKSTDLHFLYERATRPGQVSPPPGSIMIH
                     PFGRQCIGWKHAAEHDVDMALFDATRHYRIDVERTVLIGFSMGGAGAWHLGAHRASEF
                     VAVSPGAGFAETARYQRLTPDKYPPWYEQKLWGLYDVPNYTRNFFNTEVVAYSGELDR
                     QIQAARVMEEAFQAEGRKLTHLVGPGVEHKYEPQTLAELMKRLDALAIKGRPETPQKA
                     HLQTRTLRYPGQAWILATALDEHWVDSRVDAEVVNPKHVRATTKNVRRLELSPLADMR
                     GVTIEIDGTKVEIRSERSKPFELHSFASQLPRQHSVAYLTKGDKGWVEETVEPRTFSD
                     EKRPGMQGPIDDAFNSEFLVVVPTGPFASPAFQAWVEFEQQHFVARWRALMRGQPRVK
                     KDTEVTEWDQQNFNLILWGDAESNRVIEKFAQFVPLKFEKGTWQFGKQKYDGSKFAPA
                     LIHPNPLGIGHYVVLNSGLTFREGHDRTNSQQNPKLPDWAIIDITQPPDAFAPGRIHD
                     AGFFGEHWELKSPPKAP"
     gene            5793..>6028
                     /locus_tag="VFB80_10780"
     CDS             5793..>6028
                     /locus_tag="VFB80_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN34298.1"
                     /translation="MSAADRQKWNAKYAVEIPPREPSAVLVGLDRFLPKVGRALDAGG
                     GGGRNAIWLAQRGLDVTLADISAKGLAISRERAS"
BASE COUNT         1000 a         1921 c         1939 g         1168 t
ORIGIN      
        1 ccaggaatct gccgtgaaga gcagctcccc ttcggcgcgg aaggcgagcc cgtcgatcca
       61 ggcgtgatgg ccgcccagcg ccggcaattc cgccggctcg gcttccacgt tccaccgccg
      121 cacattgccc gtgtagccgc cggccgccag caccttgccg caggggctgt agcgggccag
      181 ccgcacctgc ggatcggccg tgatcgtttt cacgtccttg gcgggctcgt attttttgag
      241 cagctctttg gagtcggtca tgccagcagc tccttcacgg gcttcatgtc ttcgtgcgcc
      301 agaggcagcg gctggccgcc gttgtcgtac tggacctgct tggtgtcgat gcccaggcag
      361 gcgaaccagg tgtggaacag ggagccgata tcgtgttcgt cccctttgac gtaggtgccc
      421 tgctcgttgg tttcgccgac gaccgcgccc cgtgcgaggc cgcagccggc catggcgatc
      481 gaccaggctt cgggccagtg gtcgcggccg atgtggccgt tgattcgcgg cgtgcggccg
      541 aattcgctca tcgcgatcac cagcacgttc tcgagcaggc cgcggtcatc gaggtcgctc
      601 acgatcgagg cgaacgcctg gtcgaacttg ggcatcaggc taaggtggcc gttgaagttg
      661 tcgccgtggg tgtcccaccc gtagctgttg accttgacga aggtcacgcc cgcttcgagc
      721 atcttgcggg cctgcagcat gtggcggccg atctcgtgcg tgccatagcg ctcgacgtct
      781 ttggagtcga acctagtgct gtcgaagagg tcctgccgct tcatgagcgt gcgggccacg
      841 tcatagacgt agctcgtcgc ctcgcccatc tcgggccggc ggcccttggc atagcgggcg
      901 ttggccgcgg cttgcagcac ggcccgctca tcggcgtcgt gcggcggcac ttccggcggc
      961 gcgacgaggt tctccggcgg cttgccgtcg ccgaaggcga ccgcaccgta cttggggccg
     1021 atgaagcccg cgtcgccgtg gatgaacccg ccgctgccgg gcttgatcca gacgtagggc
     1081 ggcaggccgc tgtcgccggg gccgagcagc ttggccacgg ccgagccgaa gtacggatag
     1141 gtcacgccgc gattcttcgg gtcgccccgc tggatgcggg ccacgccggc cgagtgcgag
     1201 ttgtcctgcg tgtggacgct gcggatgacc gccagcttgt ccatgatttt ggccgaccgc
     1261 ggcagcagct cgctgacgtg cacgcccggc acgctcgtcg gaattgcccg aaaaggcccg
     1321 ccataggtcg tgttcggctt cggatcccag ctttcgagct ggctcatgcc gccgtcgatc
     1381 cagatgaaga gcacctgctt gcgcttttgc ttcagttcgt cggctaccgc agtccgcagc
     1441 agcgagccca ggccgagtgc acccgccgcg ccgagaatct gccggcggga gagggcgtgc
     1501 tcgcggggcg tgcagaggtg ggagcatttc atggtcaaag ccgcgactag cggtcgcggt
     1561 gcatggtgga ccgcgaccac tggtcgcggc tttgagcaat tagtggttcg tacaaaactc
     1621 gttcgaggca atcaggctcc aaatcaggtt gctgacggcg gactcgcgat ggtcggcacg
     1681 cggtgtcagg tactcgccga ccgcagcggc ttcttcggcc gttggcgagc gggacagcac
     1741 cgcgaggtac agctcgtccg cgagcttggc cgcgtcgctc tccttcagca accgcgcggt
     1801 caagctgtcg ccggacggct tcagccaggc gagcagcgtc gtgtcgtgca tcaaaaacag
     1861 cgcggccttc acggtcggat tgtggccgac ttccggctcg cgggctggat tggccagggc
     1921 cttgtcgaac ctggtctgcc aatcggtcca ggtcttggcg gccgggtcga tcgccagcgg
     1981 ctggccgtca cccagcgcct gctggatgct ggccagcatc tgctcgctgg agagcggctt
     2041 ctccagcgcc gcccggtaac tctgcggcaa gacctcgccg gccaataccc ccgaccgctg
     2101 atacgtctgc gacaaggcga tctcgcggat cagccaccgc atgtcgaact gatgggcggc
     2161 cagctcgctg gccagcagct cgagcagttc cgggtgcgac gcgggattcg ccttgtgcga
     2221 aaggtcgagc ggatgcacca ggccgcgtcc cagcatcagc caccacaggc gattggcgat
     2281 gttcttcgta aacagctgat tctcgccccg cggcagctgc tcgccgagca gcttcagcgt
     2341 gtcgaacttc agcttgcccg ggaacttcgt cttcttgtcc ggcggctgct cgaactcttc
     2401 gcctttgccg aaagttggaa tctcgatctc ctgccccagc gggagcttcg gccccaccgc
     2461 cagcggcttc tggagaaaga ccgacatgaa ctcgaccttc ttgtcgagcg gcttcagggc
     2521 gaccgccgga aactgcacgt cggtgcggat cgtcgtgttg ccgacgaacg ccagcatgcc
     2581 gtgatagaac tcctgcttgt aatcctcgac gaacaggtgg tcgtggcact gggcgcattg
     2641 cacgtcgatg cccaggaaat gccggcctac gtcgcggacc atccccggaa tatcgaccgg
     2701 gttctcgcca tagtgctcca gccgcttggt gtaccagagc gccgccccgc gggcggtttc
     2761 gctctcgctg tcggggctga gaatgtcgcg gaccatctgg tcccagggct tattggcctc
     2821 gaacgatgct ttgaggaact tctgccactc ggcgtggtcc cccagccgct ccatgagcat
     2881 gacgtgaaag acctgcgtca tccggcggac gtggtcgccg ctggccaaga gcttgtcgat
     2941 cagcttctcg cgcttgtcgg ccgccggatc ggccagaaag gcccgcgtct cttccaccga
     3001 gggcaggcgg ccggccagat caagatagat gcggcggatg aactcggcgt ccgacgcctt
     3061 ctcggccagc ggcccgccgg ccttggcctc aatcagggcg tcgatccgcg cgtgcaaagc
     3121 agccgcatcg tcgccccatg cggaggcggc ccaaggcagc gccaaacaga tggccagcaa
     3181 acggggtagc gccatcgcat tcatggcggg atctcaggag cgaggaaagg cgggatcgaa
     3241 tcggcgggcg ttgatcttag tgtaaccgct cgccgcatgg caaagaaaga ggctttcgtc
     3301 tgcgcaatcg gcactctctg gtctggttcc caagctccgc ttgggaaccc acttcccgaa
     3361 actctgcttc gcctcaagtg gaagcggagc ttccagacgt gcgtgaccaa gcggagcttg
     3421 ggcacgagag aagaggtttc ggctgggccg gcgggacgcc cgccccacac gtcacggcgc
     3481 cttgggcggg gacttgagct cccagtgctc gccaaaaaac cccgcgtcgt gaatccgccc
     3541 cggcgcgaat gcatccggtg gctgcgtgat atcgatgatc gcccaatccg gcagtttggg
     3601 attctgttgc gagttcgtcc ggtcgtggcc ttcgcggaac gtgaggcccg aattcagcac
     3661 cacgtaatga ccgataccta gcgggttggg gtggatcaac gccggcgcga acttgctccc
     3721 gtcgtacttc tgcttgccga actgccaggt tcccttctca aatttcagag gcacgaattg
     3781 ggcgaacttt tcaatgacgc gattggactc ggcgtcgccc cagagaatca aattgaaatt
     3841 ctgctggtcc cactccgtaa cctcggtgtc cttcttgacc cgcggctgcc cgcgcatcag
     3901 cgcccgccag cgagcgacaa agtgctgctg ctcgaattca acccacgctt ggaatgctgg
     3961 gctcgcgaac gggccggtgg gcacgacgac gagaaattcc gagttgaacg cgtcgtcgat
     4021 cggcccctgc atgcccggcc gcttttcatc cgaaaaagtc cgcggctcga ccgtttcttc
     4081 cacccagccc ttgtcgcctt tggtcaaata ggcgacggaa tgctggcggg gaagctggga
     4141 tgcgaacgaa tgcaactcga acggcttgga gcgctccgat cgaatttcga ccttcgttcc
     4201 atcgatttcg atggtcaccc cgcgcatatc cgcgagcgga ctcaactcca gacgacgaac
     4261 gtttttcgtc gtcgcccgca cgtgtttggg attcacgact tccgcatcga cgcgggaatc
     4321 gacccaatgc tcgtcgagcg cggttgccag gatccaagcc tggcccggat aacgcagcgt
     4381 ccgcgtttgc aaatgcgctt tctggggagt ttccggtcgg cccttgatcg ccaaggcgtc
     4441 cagtcgcttc atcagttccg cgagcgtttg cggctcgtac ttgtgctcga caccggggcc
     4501 gaccaggtgc gtcagcttcc tgccctcggc ctgaaatgcc tcttccatca cgcgagcagc
     4561 ctgaatttgc ctgtcgagct cgccgctgta ggcgaccacc tccgtattga aaaagttgcg
     4621 ggtatagttc ggcacgtcgt agagccccca caacttctgc tcgtaccagg gcgggtactt
     4681 gtcgggtgtc agtcgctgat aacgggccgt ttcggcaaag ccagcgccgg ggctgacggc
     4741 gacaaactcg ctggcacggt gggcgcccaa atgccaggcg cccgcgcctc ccatcgaaaa
     4801 gccaatcaag acggtccgct cgacgtcaat tcggtagtgc cgagtcgcat caaacagtgc
     4861 catgtcgacg tcatgttcgg ccgcgtgctt ccaaccgatg cattgtcgtc cgaagggatg
     4921 aatcatgatc gagcccggcg gcggcgaaac ttgtcccggc cgcgtcgccc gctcgtaaag
     4981 gaagtgcagg tcggtgctct tgtcgccgcg cccatgcagc cacacataga ctcggcacgg
     5041 cttggaaaaa tcgtgatcgg gcggaatgac gacgccatac ggctgaacgg agccgtcgat
     5101 cttggaccga tagcccctca cgacgagtcc cgtagcccgc gaccagggga cttcgtcttt
     5161 cgccatcgca tcgagtcgct gattggcctg gtcgagcgcc caatcagcct tttcaaaatc
     5221 cttcggaacg tagaactcac gatggagcag cgcgaattcc accgccttta cgaatacctc
     5281 gacgtcgaga ggatagcgcc acaagcgatt gtgtggtgag acctttcccg tgagtacttc
     5341 ctctttgagt tcttcatcct gcaagccttc cacatcagac tcgaatttct tgagccgttg
     5401 cttcgtttcc gcgagttgcc tttccagccg cgtgcgcacc tcggcgggaa tctccaggcc
     5461 ttcgggcggc aggacgcggg gaattggcgg gatcgcatcg gcgccgcgcc cggcagttga
     5521 gagcgaggta gcggccgcga gcagcgcggc gacgaagagg cggtggggca tgggagtgcc
     5581 tggcgggaga aaggggcggt cacattttaa cttggtagca agcttcggtc gcccttacag
     5641 ggctcaaatc gcttgggcca gccgtatcct agggcgtcgc tcgctgcgct cgcttgccct
     5701 aggctggtgt aggttgccgc gttgccgcac cgagccacct cccttgccgg ttcgtcgcgt
     5761 gtgttggtcc ccatagtatc ggctagttaa caatgtctgc tgccgatcgc cagaagtgga
     5821 acgccaagta cgcggtggag attccgccgc gcgagccttc ggcggtgctg gtggggctcg
     5881 accgcttcct gccgaaggtc gggcgagcgc tggacgcggg gggcggcggc gggcggaatg
     5941 cgatctggct ggctcagcgc ggattggacg tgacgctggc cgatatttcg gccaaggggc
     6001 tggcgatttc ccgcgagcgg gcttcgga
//
LOCUS       DASRJB010000404         6493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4061646, whole genome shotgun sequence.
ACCESSION   DASRJB010000404 DASRJB010000000
VERSION     DASRJB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4061646"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFB80_10785"
     CDS             complement(<1..328)
                     /locus_tag="VFB80_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34299.1"
                     /translation="MRSPPAEIPRSEPQSGGFGRLARRIATWTTNLLATAIVLAAGLA
                     MGWQVLAWWRERPEAEAAAALPPEAALPAIAESREFWTRGGPLRIERVRGDAAAAQAA
                     MREFCRQ"
     gene            complement(380..1333)
                     /locus_tag="VFB80_10790"
     CDS             complement(380..1333)
                     /locus_tag="VFB80_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34300.1"
                     /translation="MRQRQQHFARRRGLAPLELVLAIPILMFVLALSVIFGAVTCWKI
                     RGEVVARDAIFSSRWPGNGGIAGKEWRVPLATRGVRGGPALTELDHPVFQNELIRGPL
                     PNGIRVDDEMLDPKNHARIGVSHLRRDPPALGKIGPYQVDVEQPLLDGKWQFWQLGYG
                     GNTSRRLRRLYDLLNVPGAQGQKAIYQQSAQQTQAIYMRPALFVLDRDDEFIAWYGWP
                     APDFHPRLPRFCSLDVQDVQQRYVERHIPRIDSIPRPSNPRYDPPGVPRRMTQSFLSM
                     YRQQLRILENSPPPLSSAQQAQIAVLKQKIQILEDFLATLN"
     gene            complement(1369..2727)
                     /locus_tag="VFB80_10795"
     CDS             complement(1369..2727)
                     /locus_tag="VFB80_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZN34301.1"
                     /translation="MSCVKSIADLPISRLHRDERGSISLATVFALLLLTILLGMVINT
                     GRQVDNKVKLQNAADASTYSGGVVLARGMNTVAFTNHMLAETFALTAFMREARDRHAE
                     PFIPDILAAWARIAPILGRSGFPKFVALAGQIPAKTQAEQQMVTAYGNWMAASSELIL
                     PVLEEILAQEMIPQFQREVVAVTPGLAQTAAARIADMHTGNPSQRDQPRGPVVGVFWR
                     TLLDPVGGASEGFLGTIPAVDPATDTSSEAGRYRMSAIRARDNYARHYLGLWNGELMA
                     PFDQYAKMSQFGSLWRSFTCGQLTQLLAEYPDRNFPHLIRETWRDAPQSDWMNLDYMF
                     VGVAYRLKVQPAAPRLFPDSLAADNQAFTQGMLFAPRRRPISLWWNEFGPHFVYNNAP
                     RHWDLWNENWSFKLVPALSSSIPPILQMPPQTPFAAAAANNRLPNLQALDTEDFRRMT
                     TH"
     gene            complement(2714..3637)
                     /locus_tag="VFB80_10800"
     CDS             complement(2714..3637)
                     /locus_tag="VFB80_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34302.1"
                     /translation="MLRAVLIDAWPLAAALLLICGGLATLARLLRAGPPRPLAMLHAD
                     QQGGVQSLSFVLTVPVFIMLMMLAVQITQLMIGLVMVHYAAYAAARSASVWFPSRIEP
                     GFMYGENRAGLRVLERLEDGGEVYRIQPPEPGRGKFEQIRRAAALACLPIAPSRDLGL
                     ANGDSMTSAIQNVFAAYAPDEVASNPRIAQRLANKWAYANDATTIEIRTFHRDWPPLW
                     KLVPGPEGQYNEYEIGWQDQIRVTVIHQFALLPGPARLLARQANQSRYADTVSPQIRQ
                     RQGVYSLPLSATACLVGEGERSLIPRVYELR"
     gene            complement(3709..4290)
                     /locus_tag="VFB80_10805"
     CDS             complement(3709..4290)
                     /locus_tag="VFB80_10805"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN34303.1"
                     /translation="MLATLLLVALVLTAAVTDAARHKIYNWTTYPGMLAGLALAALGA
                     MCEWLAPDAAAAWRPLVGWLSWTDALGGFLVCGCLMAVCFVFFPMGGGDVKLLAMVGA
                     LAGLEKGLDILLWTFIFGGCLGLTILIWRFGPLRLLKRAWELLLGVVTLGTVLRPPNE
                     EKAVLQWPLFLGPCAAAAMAAALIPWPALANYM"
     gene            complement(4306..4521)
                     /locus_tag="VFB80_10810"
     CDS             complement(4306..4521)
                     /locus_tag="VFB80_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34304.1"
                     /translation="MSLKQPPALARLLAKIHRDERGGVSIETVLIIAAIALPILIFLI
                     RVGWPRLKAFFNTGMQDLETGSTNAQQ"
     gene            complement(4551..4772)
                     /locus_tag="VFB80_10815"
     CDS             complement(4551..4772)
                     /locus_tag="VFB80_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34305.1"
                     /translation="MGLIRTAIRRRKALTPALSKRATGRWRRGSATVDYFLLVCIVLP
                     LATFLMWFAPRAIQSVYDLLTVVVAWPFM"
     gene            complement(4763..5866)
                     /locus_tag="VFB80_10820"
     CDS             complement(4763..5866)
                     /locus_tag="VFB80_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN34306.1"
                     /translation="MSRTAIFEASLAYFLEPIARFLEDESVTEIMVNGPDRIFIERRG
                     KLELTPARFSDEDALISAIHNLTQYVGRDIGPEQPILDARLPSGARVHAVIPPASRTG
                     PCLTIRKFRPDVLSLADIVRLGTVSPAAREFLEICIRLRKNVIVAGASGAGKTSLLGA
                     LSEAIPHDERILIIEDTTELRLKQPHVVQLESRSASGGEGVTLRQLFVAALRMRPDRI
                     LVGEVRGGEALDMLQAMLSGHSGGLSTVHAASPRDALVRLETLSLMSDIELPVYVARA
                     QVASAVHLVVQITRFPQDGSRRVTRISEIRGLASDGQYQLADLFVLEMKGTSRDGRLQ
                     SSLEPTGEKPSFAREPQQYGLTDLIHTSEALWG"
BASE COUNT         1155 a         2097 c         2171 g         1070 t
ORIGIN      
        1 tctgccggca aaattcgcgc atcgccgctt gggcagcggc cgcatcgccg cggacgcgct
       61 cgatccgcag cgggccacca cgcgtccaga actcgcggct ctcggcgata gccggcagcg
      121 cggcctccgg cggcagcgcc gcggcggcct cggcctctgg ccgctcgcgc caccaggcga
      181 gcacctgcca acccatcgcc aggccggcgg cgaggacgat cgccgtggcg agcagattgg
      241 tcgtccaggt cgctatccgc cgggcgaggc gaccgaaacc gccggactgt ggttcgctgc
      301 gcggtatttc tgccggcggg ctgcgcatgc cacagccacc caagctttcg actgtggccc
      361 gcgtgtccat ataccgagct tagttgagcg tggccaggaa gtcttcgagg atctggatct
      421 tctgcttcaa gaccgcgatt tgggcctgct gggcggatga cagcggcggc ggcgagttct
      481 ccaggatccg cagttgctgc cgatacatcg acaagaacga ctgcgtcatt cgccgcggca
      541 cgcccggggg atcgtaccgc gggttgctcg gcctcgggat gctgtcgatc cgtggaatgt
      601 gccgctcgac gtaccgttgc tgcacatcct gcacgtccag gctgcagaac cgcggcagcc
      661 gcgggtgaaa gtcgggggcg ggccagccgt accaggcgat gaactcgtcg tcgcggtcga
      721 gcacgaacaa cgcgggccgc atgtagatcg cctgcgtttg ctgggccgac tgctggtaga
      781 tcgccttctg gccctgtgcg ccgggcacat tgagcaggtc gtagagccgc cgcagccgcc
      841 ggctggtgtt gccgccgtag ccgagctgcc agaattgcca cttgccgtcc aagagcggct
      901 gctcgacgtc aacctgatac ggcccgatct tgccgagagc cggcgggtcg cgcctcaggt
      961 gcgagacgcc gatgcgggcg tgattcttcg gatcgagcat ctcgtcatcg acgcggatgc
     1021 cgttgggcaa gggcccgcgg atcagctcgt tctgaaatac cggatggtcg agttccgtca
     1081 gggctggccc gccgcgcacg ccgcgcgtgg ccagcggcac gcgccactcc ttgccggcaa
     1141 tgccgccgtt ccccggccag cggctgctga agattgcgtc gcgggccacg acctcgcccc
     1201 gaatcttcca gcaagtcacc gcgccgaaga tcaccgacag cgccagaacg aacatcaaga
     1261 tcggaatcgc cagcaccaat tccagcggcg ccagcccgcg gcggcgtgcg aaatgttgct
     1321 ggcgttgtcg catttccgac tcctgactcc cgacccctga ctcctgccct aatgcgtcgt
     1381 catccgccga aagtcctccg tatccagcgc ctgcaaattt ggcagccggt tgttcgccgc
     1441 ggcggcggcg aagggcgtct gcggcggcat ctgcaggatc ggcggaatgc tcgacgaaag
     1501 cgccggcacg agcttgaacg accagttttc gttccacagg tcccagtgcc gcggcgcgtt
     1561 gttgtagacg aagtgcgggc caaactcgtt ccaccacagg ctgattggcc gccgccgtgg
     1621 cgcgaacagc atgccttgcg tgaacgcctg attatccgcc gccagcgaat cggggaagag
     1681 ccgcggggcc gccggctgca ccttcaggcg ataggccacg ccgacaaaca tgtagtcgag
     1741 gttcatccag tcggactgcg gggcgtcgcg ccaggtctcg cggatgaggt gggggaagtt
     1801 tcgatcggga tattcagcca gcaattgcgt gagctggccg caggtgaagc ttcgccacag
     1861 gctgccgaac tgcgacatct tcgcgtactg gtcgaacggg gccatcaact cgccgttcca
     1921 gaggccgagg taatggcggg cataattatc gcgggcccgg atggccgaca tgcggtagcg
     1981 gccggcttcg gacgaggtgt cggtcgccgg atcgacggcg gggatggtgc caaggaagcc
     2041 ctcgctcgcg ccacccaccg gatcaagcag cgtccgccag aacacgccca cgaccggtcc
     2101 gcgcggctgg tcgcgctgcg acgggttgcc cgtgtgcatg tcggcaatgc gggcggccgc
     2161 ggtttgggcc aagccgggtg tgacggcgac gacttcccgc tggaactgcg ggatcatctc
     2221 ctgtgccaga atctcctcca gcaccggcag gatcagctcg ctggaggcgg ccatccagtt
     2281 gccgtaggcc gtgaccatct gctgctcggc ctgcgtcttg gcgggaatct gccccgcgag
     2341 ggcgacgaac ttcgggaagc ccgaacggcc gagaattgga gcgatgcggg cccaggccgc
     2401 gaggatgtcg gggatgaacg gctcggcgtg tcggtcgcga gcctcgcgca tgaaggccgt
     2461 gagcgcgaac gtctcggcca gcatgtggtt ggtgaaggcg accgtgttca tcccgcgggc
     2521 cagcacgacg ccgcccgagt aggtcgaggc gtcggccgcg ttctgcagct tgaccttgtt
     2581 gtccacctgg cggcccgtgt tgatcaccat ccccagcagg atcgtgagca gcagcagcgc
     2641 aaagacggtc gcgaggctga tcgagccccg ctcgtcgcgg tgcaacctgg agatgggcaa
     2701 gtcggcgatg gacttaacgc agctcataga cgcgtggaat gagcgaccgc tcgccttcgc
     2761 cgacgaggca agccgtggcg gagagcggca gggaataaac gccttgcctt tgccgaatct
     2821 gcgggctgac cgtgtccgcg tagcggctct ggttggcctg gcgggccagc aatcgggccg
     2881 gaccgggcag cagggcgaac tggtgtatca cggtcacgcg aatctggtcc tgccaaccga
     2941 tctcgtactc gttgtattgc ccttcggggc cgggcacgag cttccagagc ggcggccagt
     3001 cgcgatggaa cgtgcgaatc tcaatcgtcg tggcgtcgtt ggcgtaagcc cacttgttgg
     3061 ccagcctctg tgcgatgcga ggattcgagg cgacttcgtc cggggcgtac gcggcgaaca
     3121 cgttttgaat ggcggaggtc atcgagtcgc cattggccag gcccaggtcg cgcgaggggg
     3181 cgatcggcaa gcaggccagc gcggcggcgc ggcggatttg ctcgaacttt ccgcggcccg
     3241 gttcgggggg ctggatgcga tacacctcgc cgccgtcctc cagccgctcc agcacgcgca
     3301 ggccggcgcg gttttcgccg tacatgaagc cgggctcgat gcgcgacggg aaccagacgc
     3361 tggccgagcg ggccgccgca taggccgcat agtgcaccat caccaggccg atcatcagct
     3421 gcgtgatctg cacggccagc atcatcagca tgatgaacac cggcaccgtc agcacgaaac
     3481 tcaagctttg cactcctccc tgctgatccg cgtgcagcat tgccagcggc cgtggcgggc
     3541 cggctcgcag cagccgcgcc agcgtggcca gcccgccgca gatcagcagc agcgccgccg
     3601 ccagcggcca ggcgtcgatg agcacggccc gaagcattgc ctgaactcgt agccgaattc
     3661 gccagaattc ggacaacccg gattgctact gaattctggc gaattcagct acatgtagtt
     3721 tgctaacgcc ggccagggga taagcgccgc ggccattgcc gcagccgcac acggaccaag
     3781 gaacagcggc cactgcagca cggccttctc ttcgttcggc ggacgcagga ctgtgccgag
     3841 cgtaaccacg cccaacagca gctcccaggc ccgcttcagc agccgcaagg gcccaaaccg
     3901 ccagattaaa atcgtgagcc ccagacagcc gccgaagatg aacgtccaca gcaggatgtc
     3961 gaggcctttt tcgaggccgg ccagtgcgcc gaccatcgcc agcaatttca cgtcgccgcc
     4021 gcccatcggg aaaaacacga agcaaaccgc catcaagcag ccgcagacca ggaatccgcc
     4081 gagtgcgtcg gtccaggaga gccagccgac gagcggccgc caggccgcgg cggcatcggg
     4141 cgcgagccac tcgcacatcg caccgagcgc agccagcgcc aggccggcca gcatgccggg
     4201 ataggtcgtc cagttgtaga tcttgtgcct tgccgcgtcg gtcaccgccg cggtcagcac
     4261 gagcgctacg agtaggagcg tggcgagcat cttgttgctg ggcgcttatt gctgggcatt
     4321 cgtcgagccg gtttccaagt cctgcatgcc ggtgttgaag aacgccttca ggcgcggcca
     4381 gccgacgcgg atcaggaaga tcaggatcgg cagggcgatc gccgcgatga tcagcaccgt
     4441 ttcgatcgag acgccgcccc gctcgtcgcg gtggattttc gccagcaagc gggccagtgc
     4501 cggcggctgc ttgagtgaca tgattcggtc ctcccaggtt gattcgcggc ctacatgaac
     4561 ggccaggcca cgacgacggt cagtaaatca taaacggatt gaatggcccg cggcgcgaac
     4621 cacattagga acgtcgccag tgggagcacg atgcagacaa gcaggaagta atcgacggtc
     4681 gccgaaccgc gccgccaccg ccccgtcgcc ctcttggaga gggccggggt gagggccttc
     4741 cggcgtcgaa ttgccgtccg gatcagcccc ataacgcctc cgacgtgtga atcaggtcgg
     4801 tcaggccata ttgctgcggc tcgcgggcga agctcggctt ctcgccggtc ggctccaggc
     4861 tcgattggag ccgaccgtcg cggctggtac ccttcatctc caggacaaac aggtcggcca
     4921 gctgatattg cccgtcggac gccaagccgc ggatttcgga gattcgcgtg actcgccgcg
     4981 aaccgtcctg cgggaagcgc gtgatctgca ccaccaggtg caccgccgac gcgacttgcg
     5041 cccgggcgac atagaccggc aactcaatat ccgacatcag cgacagcgtc tccagccgca
     5101 cgagcgcgtc gcgcggcgag gcggcgtgga ccgtcgagag tccgccgctg tggccgctga
     5161 gcatcgcctg gagcatgtcg agcgcctcgc cgccgcggac ttcgcccacc agaatccggt
     5221 cgggccgcat ccggagggcc gctacgaaga gctgccgcag cgtcacgccc tcgccgcccg
     5281 aagccgaccg cgactcgagt tgcaccacgt gcggctgctt cagccgcagc tcggtggtgt
     5341 cctcaataat cagaatgcgc tcatcgtggg ggatggcctc agacaaagct cccagcagcg
     5401 acgtttttcc cgccccgctg gccccggcga cgatcacgtt cttgcgcaag cggatgcaaa
     5461 tctcgaggaa ttcgcgggcc gcgggcgaca cggtgcccag gcggacgatg tcggccagcg
     5521 acagcacgtc ggggcggaac ttgcggatcg tcaggcaggg ccccgtccgc gaagccggcg
     5581 gtattaccgc gtggacccgc gccccgctgg gcaggcgagc atcgaggatc ggctgctcgg
     5641 gaccgatgtc tcggccgacg tactgagtga ggttgtgaat ggccgaaatg agggcgtcct
     5701 cgtcgctgaa ccgggctggc gtgagctcca atttgccgcg gcgctcgatg aaaatccgat
     5761 ctggaccgtt gaccatgatt tcggtcacgg attcgtcttc caggaaccgc gcgatcggct
     5821 ccaggaagta ggccagactg gcttcaaaaa tggcggtacg ggacataaca acgcctgcgg
     5881 cgcgcacaac gaacggcccg ccggaatgct gaccaattgc ggctgcacct accttgagac
     5941 taagtaagtt cgctggtgat tgtcaacttt cttgccttgg ttcgcgtccg tggttctgcc
     6001 gcggcgccaa aatccaagaa ttaggcactg agcccgaata agggagctag ttggcgcatc
     6061 tatacagttg tggcaagaag ctgcaaagag ggggcgggcc gcggagcttt ggcaatttgg
     6121 tcgtttgtag agagcggcgc gcataatgcg catagtcgtt tggcgcatgc gcatgacgta
     6181 agatgcagtg cagacagggg ttagacgctg aaacgccgcc tggacaactt tcttcaaaga
     6241 tgcgcgcata ggcccgagag cggctgccgg ggcgtcctgc gcggcgcgca taatgcgcat
     6301 atcgactggg ccgcgcgcat gaccgcaagt tcttgtggcg acaggagtta ggcgcagaat
     6361 cgccgtctgg aagtttttct cggatagtcg cgcatcggcc gaccgcctac ggcgccggca
     6421 gcaggcagtg gcggttggcc atggccacgc cgctaatgag ggctccgacg atgccgagat
     6481 agccctggtc ggt
//
LOCUS       DASRJB010000405         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_963433, whole genome shotgun sequence.
ACCESSION   DASRJB010000405 DASRJB010000000
VERSION     DASRJB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963433"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFB80_10825"
     CDS             complement(<1..311)
                     /locus_tag="VFB80_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZN34307.1"
                     /translation="MFRLSRRRFLSASAAAAGALGLGAYANPAPAQPSRSPSERLRIA
                     GVGTTGRAGADLSEVSSQDIVALADVDDKLMEKGSARYPAARKYRDFRVMLEREADKI
                     DA"
     gene            523..906
                     /gene="rsfS"
                     /locus_tag="VFB80_10830"
     CDS             523..906
                     /gene="rsfS"
                     /locus_tag="VFB80_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZN34308.1"
                     /translation="MALTLSQPVVRTASDRSLQLALAAAREAEDNRGQDLVVLDMREQ
                     TTAFDYFVVATGTSNRQLRAMSDAIDDVLQKGHAHRRLGREGYEQSHWILLDYGSVVV
                     HLFDKNAREFYRLEELWAGTTRVER"
     gene            complement(1039..1767)
                     /locus_tag="VFB80_10835"
     CDS             complement(1039..1767)
                     /locus_tag="VFB80_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34309.1"
                     /translation="MSVTHTFENDSRAWSPIGWAFHVWSWIALLVLFVLLEVFVDPLT
                     ASLVLCLKLGWRDLLVALRLRGHDDPRIVHAISLYCLAQACFKVALAGVLVWALIVAC
                     ELLLGIPQPVERFLAGFCLLFSGLILGALTVGFAAVRTVEYRVRPWLDGTLYKHLLDR
                     SQRLTCQGTFNRVPALLRLGMLLATLVLAPGVYAGYHLLVVKGNLAGIMGPVIFITLW
                     WHGVKPMWTALKQVASSPAECWDA"
     gene            1865..>2356
                     /locus_tag="VFB80_10840"
     CDS             1865..>2356
                     /locus_tag="VFB80_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34310.1"
                     /translation="MPRLLTITGFLVLTAPALAAEPGLTMSWEKNYLTIRGDFPGQEL
                     KILYLEAYCRPGSTDRDWKETVIPHTAEQLEASEGGRTIKLRDKLADGVVVEHSLTAS
                     TDEIDFRLVAHNPTDKASEAHWAQPCIRVDRFTGTTNTDARTLVPAYARKCFLFLDGK
                     LTRL"
BASE COUNT          434 a          794 c          755 g          373 t
ORIGIN      
        1 gcgtcgatct tgtcggcctc ccgctcgagc atcacgcgga agtcgcggta cttgcgggcc
       61 gccggatagc gagccgagcc cttctccatc agcttgtcgt ccacgtcggc caaagcgacg
      121 atgtcctggc tcgaaacctc cgacaggtcc gccccggccc gcccggtcgt gcccacgccg
      181 gcgatccgca gccgctcgct gggcgaccgc gacggctggg ccggcgctgg attagcgtat
      241 gctcccaggc ccagcgcccc ggcggctgcg gcagaagcgg ataggaagcg gcggcgggag
      301 aggcgaaaca tagctttttt cctggcgttg gcgagggttt tcaccggtct ttgatcctgg
      361 atcttctcgc cttgcaacat actcctggaa tttgcggaat tcaacgcact cgccgcgcgg
      421 ctcgccgcgc cgaattcacg aacctctcgg ctgacttcgt tctcccgtct gggtagaatg
      481 cagttggtgg gcgattgatg cccttattcg aaaggagcgt gcgtggcact aaccctgagc
      541 caaccggtcg tgcggacggc ttccgatcgc agtttacaac tcgccctggc ggcagctcgc
      601 gaagcggaag acaaccgcgg acaagatcta gtggtgctcg acatgcggga gcagacgacg
      661 gccttcgact acttcgtcgt cgccaccggc acgagcaacc ggcaactgcg ggccatgagc
      721 gacgcgatcg acgacgtgct gcaaaagggg cacgcccacc gccgcctggg ccgcgagggt
      781 tacgaacaaa gccactggat tctcctcgac tacggctccg tcgtggtcca tctgttcgac
      841 aagaacgccc gcgagttcta tcgcctggaa gagctgtggg caggtacgac gcgggtcgag
      901 cggtagcggt agggtgggtc aagcgagtcc gcgagcgctg acccacgccg cgcacgtcgt
      961 cgctccccgc gtgggtcatc ggccgcaacc accgcttcgc gtcggtcgcg gcctcgaccc
     1021 accctacacg tcctacgctt acgcgtccca gcattccgcg ggcgagctgg cgacctgctt
     1081 gagggcagtc cacatcggct tcacgccgtg ccaccagagc gtgatgaaaa tcaccggccc
     1141 cattatgccg gccaggtttc ccttcaccac cagcaggtgg tatccggcat agacgcccgg
     1201 cgccagcacg agcgtcgcca gcagcatgcc cagtcgcaat agcgcaggca cgcggttgaa
     1261 ggtgccctgg caggtcagcc gctggctgcg gtccagcagg tgcttgtaga gcgtgccgtc
     1321 gagccagggc cgcacgcggt attccacggt ccgcaccgcg gcgaagccga ccgtcaatgc
     1381 tcccaagatc agcccgctga acagcaggca aaagccggcc aggaagcgct ccaccggctg
     1441 cggaattccc agcagcagct cgcacgcgac gatcagcgcc cagaccagca cgccggccag
     1501 ggcgactttg aagcacgcct gggccaggca gtagaggctg atcgcgtgga cgatccgcgg
     1561 gtcgtcgtgt ccgcgcagcc gcagggccac cagcaggtcg cgccagccga gcttgaggca
     1621 gagcacgagc gaggccgtca gcggatcgac gaacacctcc agcaggacga acagcaccag
     1681 cagggcgatc cacgaccaga cgtgaaacgc ccagccgatg ggcgaccacg cccggctgtc
     1741 gttctcgaag gtgtgcgtaa cggacatcgc aaaacggtct gggggttgat tctcgcggcc
     1801 gcagacagtg tgatgacgcg tgccgagagg gtttagttcg cagtctaaga cgggagaaag
     1861 ccagatgcca cggctgctga cgattacggg tttcctcgtg ctgacggcgc cggccctggc
     1921 cgccgagccc ggtctgacga tgtcctggga gaagaactat ctcacgatcc gcggcgactt
     1981 tcccggccag gagctgaaaa tcttgtacct ggaggcctat tgccggccgg gctcgaccga
     2041 tcgcgactgg aaggagacgg tcatcccgca caccgccgag cagctcgagg ccagcgaggg
     2101 cggccgcacg atcaagctcc gcgataagct ggccgacggc gtggtcgtgg agcattcgct
     2161 caccgcgagc acggatgaga tcgacttccg gctggtcgcc cacaatccga ccgacaaagc
     2221 ctccgaggcc cattgggccc agccctgcat tcgggtcgat cgcttcaccg gtacgaccaa
     2281 caccgacgcc cgcacgctcg tgccggccta cgcccgcaag tgctttttgt tcctggacgg
     2341 caaactgacg cgcctg
//
LOCUS       DASRJB010000406         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1523323, whole genome shotgun sequence.
ACCESSION   DASRJB010000406 DASRJB010000000
VERSION     DASRJB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523323"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFB80_10845"
     CDS             complement(<1..664)
                     /locus_tag="VFB80_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZN34311.1"
                     /translation="MPYKIGPHDGPRARIVASGIGLLAGAIALLLRWYLSPVLGERAL
                     YSSFLPAVLIAAYFGGLWPGLLVTIFCAAAANYFLVDPHFSLALEGPGDATALVIFAL
                     TGAFISGLCESLHRAQRRLADEERRRAEAALRQSEERFQQLIQFSSDIIGFFAADGTI
                     LYQSPSIERVLGYHPGERIGKNVFRDPLVHPDDLATKRAFFEKVKSSAGSPVTGEFRL
                     RHL"
     gene            879..>2147
                     /locus_tag="VFB80_10850"
     CDS             879..>2147
                     /locus_tag="VFB80_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN34312.1"
                     /translation="MAHLLLIDDDPVLVPEQVRQAFPAPRFRVEVAATGAAGLQRLNQ
                     DPADVILLDLRLPDQSGLDVYQQIRQIDARIPVVFVTLAKSADTAIEAMRDGAYDYLF
                     KPLDLTQLKQVVGEALDVARRMRQPAVVAETAPDPDVEGAIVGSCPALREVYKAIGRV
                     ADQDVPVLITGESGTGKELVARAIYQHGHRAKAPFLALNCAAIPESLLESELFGHEKG
                     AFTGADRRRIGKFEQCNGGTLFLDEIGDMPLASQGKILRLLQEQAFERVGGSETIRTD
                     VRLIAATHRDLPAQAAEGKFRSDLYYRLGVFAIHLPPLRERGGDLPLLVQHLVRRYSR
                     ELGREVQDVAPEALARLRAYSWPGNVRELQSVLKQALLQARGTTLLPAFLPELPGGPQ
                     SPAVSPPTGDNLALEAFIDQRLVPDTNDLYA"
BASE COUNT          387 a          681 c          713 g          366 t
ORIGIN      
        1 cgagatgccg caggcggaac tcgcctgtga ccggacttcc ggcgctgctc ttgaccttct
       61 cgaagaaggc acgtttcgtg gccagatcgt cgggatggac cagggggtcg cgaaagacat
      121 ttttgccaat ccgctcgccc ggatggtatc cgagcacgcg ttcaatggac ggcgattggt
      181 agaggatggt tccgtcggca gcgaagaagc cgatgatgtc cgaggaaaat tgaatcagtt
      241 gctgaaaccg ctcttccgac tgtcggaggg ctgcctcggc ccgccggcgc tcctcgtctg
      301 cgaggcggcg ctgggcccgg tggagagatt cacacaatcc gctgatgaag gcgccggtca
      361 gtgcgaagat gaccagggcc gtggcgtcgc cggggccttc cagggcgagc gagaaatgcg
      421 ggtcgaccag aaaatagttg gcggccgcgg cgcagaagat cgtgaccagg agtccgggcc
      481 acaatccgcc gaagtaggcg gcgatcaaga cggccggcag gaacgagctg tagagggccc
      541 gctcgcccag caccgggctg aggtaccagc gcagcagcag cgcgatcgcc ccggccagca
      601 atccgatgcc ggacgcgacg attcgagcgc gcggtccatc gtgcgggccg attttgtagg
      661 gcacgagcag acctcacgag agtgacgcgt ccattcttga cgcttgaccg cgcccgaaac
      721 aagtaacgcg tgacggcatc aatgcgaatg caatgccaaa ttagaccggt aacacgccgc
      781 tcggtatatc ctcgaatggc gtcgttatgc ctggacaggc cggaccccca cactgacaat
      841 acgtatctgc atccgtgatg ccccagaggc gagccctcgt ggcccatcta ctgcttatcg
      901 acgacgatcc ggttctggtc ccggagcagg tgcgacaggc gtttccggct cctcgtttcc
      961 gtgtcgaggt ggcggcaacc ggcgccgccg gactgcagcg actcaaccag gatccggcgg
     1021 acgtcattct gctcgacctg cggctgccgg atcagtccgg attggacgtc tatcagcaga
     1081 tccgccagat cgacgcccgc atcccggtag tcttcgtcac gctggccaag tcggcggaca
     1141 cggccatcga ggccatgagg gatggggctt acgactatct gttcaagccg ctagacctca
     1201 cacagctaaa gcaggtggtc ggggaggcgc tggacgtggc tcgccgcatg cgccagcccg
     1261 cggtcgtcgc cgagacggcg cccgatccgg atgtggaggg cgctatcgtt ggcagttgcc
     1321 cggcgctgcg cgaggtctat aaggccatcg gccgcgtcgc tgaccaggat gttccggtgc
     1381 tcattaccgg cgagagtgga accggcaagg aactcgttgc ccgcgcgatc tatcagcatg
     1441 gccaccgggc caaagcgccg ttcctggccc tgaattgcgc cgcgattccc gagagtttgc
     1501 tggaaagcga gctgttcggc cacgaaaaag gcgcatttac cggcgccgat cgccgccgga
     1561 tcgggaaatt cgagcagtgc aacggcggca cgctctttct cgatgaaatc ggcgatatgc
     1621 cgctggcctc gcaggggaaa atcctgcggc tgctgcaaga gcaagcgttc gagcgcgtcg
     1681 gcggcagcga gaccattcgg accgacgtgc ggctgattgc ggcgacgcac cgcgacctgc
     1741 cggcgcaggc ggccgaaggg aagtttcggt ccgatctcta ttaccggctc ggcgtctttg
     1801 cgattcatct gcccccgctg cgcgagcgag gcggcgactt gccgctgctg gtgcagcacc
     1861 tcgtgcggcg ctacagccgc gagctgggcc gcgaagtgca ggacgtcgcc cccgaggccc
     1921 tggcgcggct gcgggcttac tcctggccgg gcaacgtccg ggagctgcag agcgtcctga
     1981 agcaggccct gctgcaggcc cgcggcacca ccctgctgcc ggcgtttctg cctgagctgc
     2041 cgggcggtcc tcaatccccc gcggtttcgc cgccgactgg cgataatctc gcgctggaag
     2101 cctttatcga tcagcggctc gttcccgata cgaacgacct ctatgcc
//
LOCUS       DASRJB010000407         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_676565, whole genome shotgun sequence.
ACCESSION   DASRJB010000407 DASRJB010000000
VERSION     DASRJB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676565"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1819)
                     /locus_tag="VFB80_10855"
     CDS             complement(131..1819)
                     /locus_tag="VFB80_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN34313.1"
                     /translation="MHRSCKAWSLAFILVIAAGPPLAAQDAIAPDAALVAAAERAITE
                     PALRGHIRFLAGDLLEGRGPGSRGDELAQQYIAAQFESLGLRPAAPGGGWFQRVPLVG
                     VTTHVPAALTFRHEAKSLELKRHDDCVIASGKAVPQVAVENAEIVFVGYGIEAPEFQW
                     NDFKGADLRGKVLLMMNNDPEGDPELFAGKRRLYYGRWDYKYASAARQGAVGALIIHT
                     TPSAGYPFQVVQTSWSGEEFELEESAGPRLDLKGWLTEDASKRLAALAGQDLDKLRAA
                     AERRDFKPVPLGVRLSASLTCDVRQKQTGNVLGLLPGSDPARSPQAVIFMAHHDHLGL
                     AAERNAAGDNIYNGAVDNASGTAALLAIARACASLPKPPARSILLAAVGAEEQGLLGS
                     LYLANHPPLPAGNLAAVVNIDGLNIIGPTRDVNVIGLGKSNLDELVEGVARWQKRVVT
                     PDLFPDRGYYYRSDQFSLAKVGVPGVYLHSGINVIGKPEGWGKQQLEEWIQTKYHQPS
                     DEYSDEWDLRGAVEDVRLLYYVGLLAAQRPELPRWNSGDEFEAARKAALEALKK"
BASE COUNT          300 a          713 c          671 g          331 t
ORIGIN      
        1 ctttcagtac gcggtcgccg ccacgcccga cggccagacg atcctcgccg gcggcgcgga
       61 cggcatcctc cgccgctggt cgggccagga gcgcggcgtc aaaaactcgt tcgcgccgta
      121 gcgggcccgc tcacttcttc agtgcctcca gcgccgcctt gcgggccgct tcgaactcgt
      181 cgccggaatt ccatcgcggc agctccggac gctgcgccgc caataggccg acgtagtaca
      241 gcagccgcac gtcctccacg gccccgcgga ggtcccactc gtcgctgtac tcgtcgctgg
      301 gctggtggta cttcgtctga atccattcct cgagctgctg cttgccccag ccctcgggct
      361 tgccgatcac gttgatcccc gagtgcagat agacgcccgg cacgccgacc ttggcgaggc
      421 tgaactggtc ggagcggtag tagtagccgc ggtcgggaaa gaggtcgggc gtgacgaccc
      481 gcttctgcca gcgggcgacg ccttccacca gctcatcgag gttcgacttg cccaggccga
      541 tcacgttcac gtcgcgcgtg gggccgatga tgttgagccc gtcgatgttc acgaccgccg
      601 ccaggttgcc cgccggcagc ggcggatgat tggccaggta cagcgagccg agcaggcctt
      661 gctcctccgc gccgaccgcg gccagcagta tcgagcgggc cggcggcttc ggcagcgacg
      721 cgcaggcccg cgcgatcgcc agcagggccg cggttcccga cgcgttatcg accgcaccgt
      781 tatagatatt gtcccccgcg gcgttccgct cggccgccag gcccagatga tcgtggtggg
      841 ccatgaagat tacggcctgc ggtgatcgcg ccggatcgct gccgggcaac aggcccagga
      901 cgttgcccgt ctgcttctgg cggacgtcgc aggtgaggct ggccgagagc cgcacgccca
      961 gcggcacggg cttgaagtcg cggcgctcgg cggcggcgcg gagcttatcc aggtcctggc
     1021 ccgccagcgc cgccagtcgc ttcgacgcgt cctcggtcag ccagcctttg aggtccagcc
     1081 gagggccggc cgactcttcc agctcgaact cctcgccgga ccaggaggtc tgcacgacct
     1141 ggaagggata tcctgcggag ggcgtcgtat gaatgatcag cgcgccgact gcgccttgcc
     1201 gcgcggcgct ggcgtacttg tagtcccagc ggccgtaata gagccggcgc ttgccggcaa
     1261 agagctccgg gtcgccctcc gggtcgttgt tcatcatcag caagaccttg ccccgcaggt
     1321 cggccccctt gaaatcgttc cactggaact cgggggcctc gatgccgtag cccacgaaga
     1381 cgatctcggc gttttcgacg gcgacctgcg gcacggcctt gccgctggcg atgacgcagt
     1441 cgtcgtgccg cttcagctcc agcgacttcg cctcatggcg gaatgtcagc gccgccggga
     1501 cgtgcgtcgt tacaccgacc agcggcaccc gctggaacca gccgccgccc ggcgcagcgg
     1561 gcctcaggcc cagcgactcg aactgggccg cgatgtattg ctgcgccagc tcatcgccgc
     1621 gcgagcccgg tccgcggcct tcgagcaagt cgcccgccag gaaccggatg tgcccccgca
     1681 gcgccggctc ggtgatcgcc cgctcggccg ccgccaccag agccgcatcg ggcgctattg
     1741 cgtcttgcgc tgcgagcggc gggccagccg caatcactaa aatgaacgcc agactccacg
     1801 ccttgcaact gcggtgcata gccgaatctc ccaggtcagt gattgtcaac atacggtact
     1861 cgaaggcgga cgtcgccgca tcaggcgcgc gaattgacaa tcgcttccgc tgggccgtag
     1921 cgtaagagcg gagggccaac ttgtgtggtc ggtgcggcgg ctggacatag cgtggagcga
     1981 tgttgcggcg ggcctggccg gctgcctcgc gccct
//
LOCUS       DASRJB010000408         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4062357, whole genome shotgun sequence.
ACCESSION   DASRJB010000408 DASRJB010000000
VERSION     DASRJB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4062357"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1466
                     /locus_tag="VFB80_10860"
                     /pseudo
     CDS             295..1466
                     /locus_tag="VFB80_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
     gene            1478..>2197
                     /locus_tag="VFB80_10865"
     CDS             1478..>2197
                     /locus_tag="VFB80_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34314.1"
                     /translation="MLLKNRICLVTGAGRGIGRSIALAFAREGARLAITARTAKELEE
                     VAGAIRSLGGEALTIAADLAEAAAPRRIVEQTTAELGPIAVLVNNAGIGSSANPRPVV
                     DFDDDFWELSLRVNLTAPYQLCKAVLPAMIQARFGRIITVASINGKIGSFHGAAYAAS
                     KHGVLGLVRTLAMEVAQDGITVNAICPGPVATVMNDKRIAYDAQRRGVSVDEIVDTIT
                     PLGRRLTPDEIAPLAVYLASDT"
BASE COUNT          361 a          721 c          728 g          387 t
ORIGIN      
        1 gtcgcgactg aaagtgacgt catctgcccg cgacgtctcc gccgcgaaca acaggccggc
       61 catgacggcc gccagcaact tgaagccggg tcgcatccgc atagtcgctc ctcatttcaa
      121 ctctatgcac cacgacggcc gaatctcaag ggagtgcccg tccctctgcg tcttccgcgc
      181 ctctgcggtg tactttcgat acaccgcaga ggcgcggaag acgcagcgaa tactggactc
      241 gcccgatgaa ggacggcaga atcaggccac ttctacatta atccgattgc cgtcatgtac
      301 atcaccgacg ttgaagccat tcttctccga ggcgatgaga cctatcgcgc cacgagcggt
      361 ggcggggagg ccgtcgacaa cggcgactgg caggtgctcg tcaaagtctc cacggacgag
      421 gggctggtgg gctggtcgga tatcgaaaca ttgcccgccg ccgccgtcgc ctgcatcaac
      481 gggccgggac tcagcgtgct cggcctgcgc tgtttccgcg atctcctgat cggcgaggac
      541 ctccatgacg ccgaaggact gtggcaccgg ctctacatcg gcagctcgta ttacggccgc
      601 cgcggcgtcg cactgcactg catttcggca atcgacaact gcctgtggtc gatccgcgcc
      661 caggcggcag gattgccgct cggcgaactt ctcggcccgc ggcgctgtga ccgcgtaatg
      721 gcctacgcct cgacgctctt tcgcgagacg ccggaggcga tgttcaccgc ggcccggtcg
      781 tacgtggagc gcggctttcg ggccgtcaag ttcggctggg gcgtgtttgg cgaagatccc
      841 ggccgcgatc tggagttggt cgccgcggcc cgcgaggccc tcggccacca gcgcgagctg
      901 atggtcgatc ccggttggta cggcgcgggc tggaaaggac cctggcagcc gcgaacgctg
      961 gaggacaaca ttcgtctctg ccagtcgctc gagccatatc gtgtccgctg gatcgaggac
     1021 ttcatccatc cggagctgtt cgacgactac gcggccgttc gccgcgccag tcccgcgccg
     1081 ctggccgccg gcgagcaatc ggcgacgacg tgggagttcg agcggctgat caagggggct
     1141 gcgtcgatgt cgtgcagccg gacttgacgc gctgcggcgg cctgaccgtc ggcttgcaag
     1201 tcgcgcggct ggccgacgaa gccagcatcg acctggtgcc gcactcctgg ctgaccgacc
     1261 tgctcacggc ctacagcctc catttgatcg gcactttcca gcgaccgctg tttgtcgagt
     1321 tcaacgtcgc ccagagcgaa ctgacccgcg gcgtctgcgg cggggcactt tgcctgaacg
     1381 ccgacggcac ggtgactatt cccagcggcg tcggcttggg tgtcgaagtg gatgagcagt
     1441 ttgtcgcggc acgtcgagtg aattagcgag gcctctcatg ctgctgaaga accgcatctg
     1501 cctggttacc ggcgctggcc gggggattgg gcgttcaatt gccctggcgt tcgcccgcga
     1561 aggggcgcgc ttggcgatca ccgcgcggac ggccaaagag ctggaagaag tggccggcgc
     1621 gattcgctcg ctgggcggcg aggcgctcac gattgcggcc gaccttgccg aagcggccgc
     1681 accgcggcgg atcgtggagc agaccacggc cgagctgggg ccgattgccg tgctcgtcaa
     1741 caacgccggc atcggcagca gcgccaatcc tcggccggtc gtggatttcg acgacgactt
     1801 ttgggaactg tcgctgcggg tgaacctcac cgccccctac cagctgtgca aggccgtgct
     1861 gccggcgatg atccaggccc gtttcggtcg gatcattacc gtggcctcga tcaacgggaa
     1921 gatcggcagt ttccatggcg ccgcctatgc cgccagcaag cacggcgtgc tgggcctcgt
     1981 gcggacgctg gcgatggagg tcgcccagga cggaatcact gtcaacgcga tctgtcccgg
     2041 tccagtcgcc accgtgatga acgataagag gatcgcctac gacgcccagc gccgcggcgt
     2101 ttcggtggac gaaatcgtgg acacgatcac gccgctgggc cgccggctta cgccggacga
     2161 gatcgcaccg ctggccgtct atctggcctc ggacacg
//
LOCUS       DASRJB010000409         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1523616, whole genome shotgun sequence.
ACCESSION   DASRJB010000409 DASRJB010000000
VERSION     DASRJB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523616"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="VFB80_10870"
     CDS             complement(<1..1815)
                     /locus_tag="VFB80_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN34315.1"
                     /translation="MPAEPVVLGEYECRTAAGPIEIDGKGDEEAWKSAQPIERFGLPW
                     LKESARPSKTATKARLLWDRDYLYFQADLEDHDLFADVREHDGKCWDNDVFELFFRPA
                     QDKPAYYEFQVNALGTKFDSIMPKGNKTFEEIVKDGVFEWKTAVVRRGTLDNRSDRDE
                     GWSVEGRIPWSDLARTGGRPTAGDVWRFALCRYDYTLGQEPELSSCAPLTQRSFHQSE
                     QYAKLKFVAAEGDKKIGIEKRVPLTTSKVVGSPDPPLPYRPVRAFPQLKLTYPIALDR
                     VPGTDTLLIIAQGRSYGATTIYKIQDDPAVTTAEPLLETPAGGTAYGICFHPKFAENG
                     YLYIGWNGSLNKAPKKTYATRYTFDREKRTIDAASELHIISWESDGHNGGDVEFGTDG
                     MFYITSGDGTSDSDTNLRGQDLTKHTAKLLRIDVDHPADGRQYSVPPDNPFVGQKDFV
                     PEAWAYGFRNPWRLAVDPTTGHIWVGNNGQDIWEQIFFVRKADNFGWSVYEGSHPFYL
                     NRTMGPQPLALPAAEHHHNVARSLTGGVVYYGKRLPELVGAYIYGDHSTGRVWAMKHD
                     GQKVEWHKLLCDTTFNITGFGLDSHGELLVADHRGNSDG"
     gene            complement(1973..2542)
                     /locus_tag="VFB80_10875"
     CDS             complement(1973..2542)
                     /locus_tag="VFB80_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34316.1"
                     /translation="MTKETKYSVRCSCGRTTPVEKSSAGGEVACQCGQRVPVPRLSEL
                     RRRAGESAFVQGTVDAIHEQIREGKLPAGDLCAFSLRPTSDVLWITVECETPYRKGGQ
                     SWAGMFFLGLAAVFCGWLSLLYLFSRREPDEMMGREVVLRLPLRIAADAQPEVRRLGT
                     AKLRELLSMVPIYAQLLGEYPQARIHGRQ"
BASE COUNT          450 a          881 c          854 g          503 t
ORIGIN      
        1 cccgtcgcta ttgccccggt gatcggccac cagcaattcg ccgtgcgaat cgagcccaaa
       61 cccagtgatg ttgaatgttg tgtcgcagag cagcttgtgc cactcgacct tctggccgtc
      121 gtgcttcatc gcccaaacgc ggccggtcga gtgatcgcca tagatatacg cgccaaccag
      181 ctccggcagc cttttgccgt aatagacgac gccgcccgtc agcgagcggg ccacgttgtg
      241 atggtgctcg gccgcgggca gggccagcgg ctgcgggccc atcgtgcggt tgagatagaa
      301 cgggtggctc ccctcgtaga cgctccagcc aaagttgtcg gccttgcgga cgaagaagat
      361 ctgctcccag atgtcctgcc cgttgttgcc gacccagatg tgcccggtcg tgggatcgac
      421 cgccagccgc cagggattgc ggaagccgta ggcccacgcc tcgggcacga agtccttctg
      481 gccgacgaac ggattgtccg gcgggacgct gtactgccgg ccgtccgcgg gatggtcgac
      541 gtcgatccgc aggagcttgg ccgtgtgttt ggtcaaatcc tggccgcgga ggttcgtgtc
      601 ggaatcgctc gtgccgtcgc ccgacgtgat gtagaacatc ccgtccgtgc cgaactcgac
      661 gtcgccgccg ttgtggccgt cggattccca ggagatgatg tgcagctcgc tcgccgcatc
      721 gatcgtccgc ttctcgcgat cgaacgtgta ccgcgtcgcg taggtcttct tcggcgcctt
      781 gttgagcgag ccgttccagc cgatgtagag atagccgttc tccgcgaact tcggatgaaa
      841 acagatgccg taggccgtgc cgccggccgg cgtttcgagc aggggttcgg cggtcgtcac
      901 cgcgggatcg tcctgaatct tgtagatggt cgtcgcgcca taggaacggc cctgggcgat
      961 gatcagcagc gtgtcggtgc cggggacgcg gtcgagcgcg atcggatagg tcagcttgag
     1021 ctgcggaaac gcccgcaccg gccggtaggg gagcggcgga tcgggcgagc cgacgacctt
     1081 cgacgtcgtc agcggcacgc gcttctcgat cccgattttc ttgtcgcctt cggccgcgac
     1141 gaacttcagc ttcgcatact gctcggattg gtgaaagctg cgctgcgtca gcggggcgca
     1201 gcttgacagc tcgggctcct gccccagcgt gtagtcgtag cggcagaggg cgaaccgcca
     1261 gacatcgccg gccgtcggcc gtccgcccgt gcgggccaga tcgctccagg gaatccgccc
     1321 ctcgaccgac cagccttcgt cgcggtcgct gcggttgtcg agcgtcccgc ggcgaacgac
     1381 tgccgtcttc cactcgaaca cgccatcctt gacgatctcc tcgaacgtct tgttgccctt
     1441 gggcatgatc gagtcgaact tcgtgcccag cgcgttcacc tgaaactcgt agtaggccgg
     1501 cttgtcctgg gccgggcgga agaacaactc gaacacgtcg ttgtcccagc acttaccgtc
     1561 gtgctctctc acgtcggcaa acaggtcgtg gtcctccagg tccgcttgaa aatagaggta
     1621 gtcgcgatcc catagcagcc gggccttcgt cgccgttttc gaaggccgcg cgctttcctt
     1681 cagccatggc aggccgaatc tctcgatcgg ctgcgccgac ttccaggcct cctcgtcacc
     1741 cttaccgtcg atctcgatcg gccccgcggc cgtgcggcac tcgtactcgc cgaggacgac
     1801 cggctcggcg ggcatcgcca aacggcagct tgccgacaat acgaatacgg cgatccaaaa
     1861 cggacgattg atggcggaca tatttggcag gcgggctagt cgctacgagc cgggcttctg
     1921 gccactttac ggctcgtccg cggaactgac aagcaagcgg caagcgcggc ggctattgcc
     1981 gcccgtggat tcgggcctgt ggatattcac cgagcagctg cgcgtaaatc ggcaccatgc
     2041 tcagcagctc gcggagcttg gcagtgccca ggcgacgaac ttccggctgc gcatcggccg
     2101 cgatccgcag cggcagccgc aacactactt cgcggcccat catctcgtcc ggctcgcggc
     2161 gggagaacaa gtacaaaaga cttagccagc cgcaaaagac tgctgccagg cccagaaaaa
     2221 acattccggc ccaactttgg cctcccttgc ggtacggtgt ttcgcattcg acggtgatcc
     2281 acagcacgtc gctcgtcggc cgcagactga atgcacagag gtcgcccgcg ggcagtttgc
     2341 cctcgcgaat ctgctcatga atcgcatcga ccgtgccctg aacgaacgcc gactcgcccg
     2401 ctcgccgccg caattcgctg agcctgggga cgggcacgcg ctggccgcac tggcaggcca
     2461 cttcgccgcc cgcactggac ttctcgaccg gcgtcgtgcg cccgcacgag cagcgcacgc
     2521 tgtatttcgt ctccttggtc atgggagaca atatacttgc cgcgtcgtgc tacggacttg
     2581 gcggcgttgg ggcgacatac ggccgccagt actgcgagcg gtcgcggaag tcgtacgacg
     2641 gatagtggcc gtcgagccag ggctgggcaa agttcactgc gggcggag
//
LOCUS       DASRJB010000410         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_396960, whole genome shotgun sequence.
ACCESSION   DASRJB010000410 DASRJB010000000
VERSION     DASRJB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396960"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFB80_10880"
     CDS             <1..748
                     /locus_tag="VFB80_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34317.1"
                     /translation="ERAGASPPPAALPIRLNQDDLEIHRTRNRPKCATVVLMDMSGSM
                     RYDGQYINVKRMALALEGLIRREYPGDFLQMIEMFSFARPTPPGKLVELMPKPVTIFD
                     SFVRLKFDMSRDDISEHRLPQHFTNIQRSLQMARQFLCAQDTPNRQVILITDGLPTAH
                     FQGNWLYLLYPPDPLTEAATLREGLLCAREGITINLFLVPSWSQSEEDIRFAYRLAES
                     TKGRVFFTAGRDLDRYVVWDYVKRKREIVA"
     gene            complement(840..2444)
                     /locus_tag="VFB80_10885"
     CDS             complement(840..2444)
                     /locus_tag="VFB80_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HZN34318.1"
                     /translation="MKATVRGWLMGCWLAVAWLPIAARGADHMPWCGDFRMACGLAAE
                     QQRLVLLHFTRDNCAPCRKVEAEVFSEPKVAQAVSQNYIPVLVHQARNPDLVTRYQVK
                     VYPTDVMVTSSGLEVYRAISPQKPAEYIMLVNQVAQQTGTSSPRQWADKLAQAAQQST
                     GGAGDKAAAGASSFAAPYSAAADQTATGMQQMADQAAGAGHAYRQQVVGAAEELHQQA
                     ADAAANARQQLAANAQQFQQQSQDAGQKWRQATQQSFDAARGAGQQTAGAAQNLAQSW
                     QQNLSGAADATRSAFVPPGAAAPTLPPTTSAPAVVQPQLPTENPWLTPAGRALLGQQT
                     PPAAPQPDAPVAAPESRPAVPVLESTAPPLVASAPPVETSPPRVESAPAAAAPPAAAP
                     SAAAPPAESRQMVAASQAPPVALDGFCVVSLIESMNWKKADVKYGAIHRGRTYLFVSE
                     EAQQKFLSNPDGFAPVLSGCDPVRFARTGELVAGKRAYGLITPDKRVFLFADEQSLKV
                     FENSPGEYAEAARQAMLRGATGNLYR"
     gene            3347..4792
                     /gene="rho"
                     /locus_tag="VFB80_10890"
     CDS             3347..4792
                     /gene="rho"
                     /locus_tag="VFB80_10890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN34319.1"
                     /translation="MGKKQFRRGSRGGRGNGYGGGGGYGNNGGGYGGYGGGGGGGYGG
                     GGGRRRPRRHYQDRPERPPFGGEAAPDGAMTAEPPPPQLDENGNPIPVPEGQQQYPSA
                     PPATETPLEPGYGLLEMHPNGYGFLRSPDNTYTRERSDPFVPGTMIERYGLRQGVMIR
                     GTVQPARRQQGPRLREITDVDGMPPEEYLNVKTFDAKTPINPDKWFRLETGPQPLTTR
                     VMDLLTPLGRGQRALIVSPPRSGKTIMLQHISMGMTANFPDVKQFVLLIDERPEEVTD
                     MRRNVKGEVCASSLDEDIESHVRLSQLVIERCKRLAEMGQDVFVLLDSITRLARAFNK
                     YVGNKGEGIQSGGLSVRAMDIPKKLFATARAFEEGGSVTVIGTALIETNSRMDDAIFQ
                     EFKGTGNMELVLDRKLAERRIFPAIDVSQSGTRREELLLDANSLHAVTMLRRTLSGMN
                     PIDAMEQLTKQLGKYKTNADFIKLIASARQD"
BASE COUNT          849 a         1578 c         1548 g          843 t
ORIGIN      
        1 agagcgagcc ggggcgtccc cgcccccggc tgcgctgccc attcgcctca accaagacga
       61 tctcgaaatc caccgcacgc gcaaccgccc taagtgcgcg accgtcgtgc tcatggacat
      121 gagcggctcg atgcgctacg acgggcagta catcaacgtc aagcggatgg cgctggcctt
      181 ggaggggctg atccgccggg aatatccggg cgacttcctg caaatgatcg agatgttctc
      241 gtttgcccgc ccgacgccgc ctggcaaact cgtcgagctg atgcccaagc cggtaacgat
      301 ctttgattcg ttcgtgcggc tgaagttcga tatgagccgc gacgacatca gcgagcaccg
      361 cctgccgcag cacttcacga acatccagcg gtcgctgcaa atggcccggc agttcttatg
      421 tgcccaggac acgccgaacc ggcaagtgat cctgatcacc gacggcctgc cgacggcgca
      481 ctttcagggc aattggctct acctgctgta tccgcccgat ccgctgaccg aagcggcgac
      541 cctgcgcgaa gggctgctgt gtgcccgcga aggcatcacc atcaacctgt ttctcgtccc
      601 gagctggtcg cagtccgagg aggacatccg cttcgcctac cgcctggccg agagcaccaa
      661 gggccgcgtc tttttcactg ccggccgcga cctcgaccgc tacgtcgtat gggactacgt
      721 gaagcggaag cgagagattg tcgcgtagca aatctcaaga accgaagatc caaagaacca
      781 aagtcagcgt tgaacttctt ttggttcttt ggttcttcgg ttctttggat tttgcctcgc
      841 taccggtata gatttcccgt ggcacccctg agcattgcct gccgcgccgc ctcggcgtat
      901 tcgccgggcg agttctcgaa aaccttcagc gactgctcgt cggcaaacag gaagaccctc
      961 ttgtcgggcg taatcaggcc gtaagcccgt ttgccggcga ccaattcgcc cgttcgggcg
     1021 aagcgaactg gatcgcagcc cgagaggacc ggggcaaagc cgtcgggatt gctcaggaac
     1081 ttctgctgag cctcttcgct gacgaacagg tacgtgcggc cgcggtggat cgcgccgtac
     1141 ttgacgtcgg ccttcttcca attcatcgac tcgatcagcg agacaacgca aaatccgtcc
     1201 agggccaccg gcggcgcttg tgaggcggcg accatttgcc gcgattcggc gggcggtgcc
     1261 gcagccgacg gtgccgccgc cggcggtgcc gccgcggccg gtgcggattc gacgcgcggc
     1321 ggcgaggttt caaccggcgg cgctgacgcg accaggggcg gagccgtgct ttccagcacc
     1381 ggaactgccg gccgcgattc aggcgccgcg accggcgcgt ccggttgggg cgccgctggc
     1441 ggagtctgtt ggcccagcag ggcccggccg gccggcgtca gccaggggtt ttccgtgggc
     1501 agctgcggct gcacgacggc gggagccgaa gtggtcggcg ggagggtcgg cgctgccgca
     1561 ccgggcggta caaacgccga ccgcgtcgcg tcggcggcgc cgcttaggtt ctgttgccag
     1621 gactgcgcca agttctgcgc cgcgcccgcg gtttgctgtc cagcgccgcg ggccgcatcg
     1681 aacgactgct gcgtcgcctg ccgccacttc tggccggcat cctgtgattg ctgctggaac
     1741 tgctgcgcgt tcgccgcgag ttgctgcctg gcgttggccg cggcatcggc ggcctgctgg
     1801 tgcagctctt ccgccgcgcc gacgacttgc tgccggtagg cgtggcccgc accggccgcc
     1861 tggtcggcca tctgctgcat tccggttgcg gtctgatccg cagccgcact gtacggtgca
     1921 gcaaagctgc tcgcgcctgc ggcggccttg tcgcccgcac caccggttga ttgctgggca
     1981 gcctgcgcga gcttgtcggc ccactgccgc gggctgctgg tgcctgtctg ctgcgcgacc
     2041 tggttgacaa gcatgatgta ttccgccggc ttctgcgggc tgatggcgcg ataaacttcc
     2101 agccccgacg acgtcaccat gacgtccgtt ggatagacct tgacctgata gcgggtaacc
     2161 agatccggat tccgggcctg atgaaccagt accgggatgt aattctgcga cacggcctgg
     2221 gcaactttgg gctcgctgaa gacctccgcc tcgaccttgc ggcagggggc gcagttgtcc
     2281 cgcgtaaaat ggagcagcac gagccgctgc tgctcggcgg ccaggccgca agccatgcgg
     2341 aagtcgccgc accacggcat gtggtccgcg ccgcgagcgg caatgggcag ccaggccacc
     2401 gcgagccagc accccatcag ccatccccgg acagttgcct tcatgacggt acttccttgt
     2461 gcgagcctga acacccggcc agatgtcgtg cgggcggaag cctgtggaat ctcatcgctc
     2521 aagatcggcc gcgcgcggcg tgccgcttgg agaaaacctg ccggttgtgc cgcaattgcg
     2581 gtcaaaaacg gcttcccgcc tcaccggccg cgccgacaga ttttccccaa aattcacgtt
     2641 cccgcatgga gttacagctt gacacgggcc gagagatcaa taagattcgc tgtagtagca
     2701 cttctgctaa ccggcaagcg tttgtcgccc ctgaaacggg cggagcaggt gtcgggggga
     2761 aaccatcccg ctcttgccaa acgcttctct gaccctggat gtgcccgctt tggcctgatt
     2821 cgctcagctt gagttggtgc attccggtct ttgctcctgg cagtcggctg cgaacaaagt
     2881 cgttcgcccg cgcggggcag ggaatgcgaa tcgcagcagg aggcggagtg gcgctaagtc
     2941 ggtcaatcgt cgggtgcatg catcgtgctg cgaattcgcg gcacgacgaa tgctgctaat
     3001 cgatgggtcg gtcgaagcgc cggttggttc ggacgaatga caccgggaac acgttcccaa
     3061 gtgtccaatc ccagacagtg ctctgtctgc acaggttacc cgcataccgt ttggaattcg
     3121 ccgcgcgagg gcaagcagcc gctatcggca gcgaatcttt gcgagaggcc gatcttcgat
     3181 agacgcggag cgttggactc ggccatggca gttggccagc cgcggttagc gctcggcatt
     3241 tcgttccccc agggacacag tttccatctc actttaggtc aaaggacctt cttccaccca
     3301 gcgcagcgcc ttgggcgcac gcgcatatct ttctggattt atgacaatgg gaaaaaagca
     3361 gtttcgtcgc ggttctcgcg gcggccgtgg taatggctat ggtggcggcg gcggttacgg
     3421 aaacaacggc ggcggatacg gcggttacgg cggaggcgga ggtggcggat atggcggcgg
     3481 cggtggccgg cggcgtccgc ggcggcacta tcaggatcgg cccgagcgcc cgcctttcgg
     3541 cggcgaggct gcccccgacg gggcgatgac agcagagccg cctcccccgc agctcgatga
     3601 aaacggcaac ccgattcctg ttcccgaagg ccagcagcaa tatcccagcg cccccccggc
     3661 gactgagact ccgctcgagc ccggctacgg cctcctggag atgcacccca acggttacgg
     3721 cttcctccgc agcccggaca acacctacac ccgtgagcgc agcgacccgt tcgtgcccgg
     3781 cacgatgatc gagcggtacg gactgcggca gggtgtgatg atccgcggca cggtgcagcc
     3841 ggcccgccgg cagcaaggcc cgcggctgcg cgagatcacc gacgtggacg gcatgccgcc
     3901 cgaggaatac ctcaacgtca agacgttcga cgccaaaacg ccgatcaatc cggacaaatg
     3961 gttccgcctg gagaccgggc cgcagccgct gaccacgcgg gtgatggacc tgctcacgcc
     4021 gctgggccgt ggccagcggg cgctgatcgt ctccccgccc cgcagcggca agacgatcat
     4081 gctccagcac atcagcatgg gcatgaccgc caacttcccc gacgtgaaac agttcgtctt
     4141 gctgatcgac gagcggccgg aagaagtgac cgacatgcga cggaacgtca agggcgaggt
     4201 ctgcgccagc agcctcgacg aagacatcga gagccacgtc cgcctgtcgc agctcgtgat
     4261 cgagcgctgc aagcggctgg ccgagatggg gcaggacgtg ttcgtgctgc tggactcgat
     4321 cacccgcctg gcccgcgcgt tcaacaaata cgtgggcaac aagggcgagg gcattcagtc
     4381 gggcggcctg tccgtccggg cgatggacat tcccaaaaag ctcttcgcca cggcccgcgc
     4441 gttcgaggaa ggcggctcgg tgacggtcat cggcaccgcc ttgatcgaga ccaacagccg
     4501 catggacgac gcgatcttcc aggagttcaa aggcacgggc aacatggagc tggtgctcga
     4561 ccgcaagctg gccgagcggc ggatttttcc ggcgatcgac gtgtcgcagt ccggtacccg
     4621 ccgcgaagag ttgctgctcg acgccaacag cctgcacgcc gtgaccatgc tccgccgcac
     4681 cctttcgggc atgaacccca tcgacgcgat ggagcagctc accaagcagc tcggcaaata
     4741 caaaaccaac gccgacttca tcaagctgat cgccagcgcg cggcaagatt agccgtgacg
     4801 acgctgcctg cgtcgtca
//
LOCUS       DASRJB010000411         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2081697, whole genome shotgun sequence.
ACCESSION   DASRJB010000411 DASRJB010000000
VERSION     DASRJB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081697"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFB80_10895"
     CDS             <1..352
                     /locus_tag="VFB80_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34320.1"
                     /translation="IPPPAMATFDAPTREFCSVRESRTNTPLQALLMLNEPTLVAAAQ
                     ALADRVMREATAPDDRVTRAMLCAVGRPLTEQEAAIFRAALARYRDRGADVAAAYALL
                     CGTILNLDEAVTRQ"
     gene            349..1785
                     /locus_tag="VFB80_10900"
     CDS             349..1785
                     /locus_tag="VFB80_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34321.1"
                     /translation="MSNIGPTPFPVTRRDLLGQMATGIGAAALGTLLSGESRGEESLP
                     KPHFAPKAKRVIFLFQSGAPSQLDLFDPKPKLADLRGTELPDSIRQGQRLTGMTSRQS
                     SFPVAPSRFGFAQHGKSGAWLSDLLPHTAGVADELCFVKSLFTEAINHDPAITFCQTG
                     AQLAGRPSIGSWLAYGLGSENENLPAFVVLVSQGTGNTGDQPLYDRLWGSGFMPSRYQ
                     GVKFRSTGDPVLYLSNPEGVDSTARRAMLDDVAALNRLNQQQFGDPEIATRIAQYELA
                     FRMQSSVPELLDMAGEDAQTLERYGPEVRRRGSYAYNCLLARRMAERGVRFVQLFHRG
                     WDQHVFLPKQIAAQCRDTDQPTAALIADLKQRGLLDDTLVVWGGEFGRTVYCQGTLTA
                     DDYGRDHHPRCFTVWLAGAGVKSGTSFGQTDDFSYNIAAEAVHVHDLHATILHLLGID
                     HTRLTYRFQGRDFRLTDVAGNVVRPVLA"
     gene            1892..>2440
                     /locus_tag="VFB80_10905"
     CDS             1892..>2440
                     /locus_tag="VFB80_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN34322.1"
                     /translation="MQPNEWNAADAIFRRYLERPRRRRLARVVDAFYQHVWATAYRVT
                     GNAADAADITQDVFLQLLLQPPRPESVRCPRAFLAWLVVGRASGLRRSAKRRKAREQS
                     ALRQAADEGIVSADIEALRDCVEDLPEDLRAAVALRYFAGLKNSDVALATGVGERQVE
                     KRLEEARAILRARLGPTAAPVLL"
BASE COUNT          388 a          845 c          823 g          384 t
ORIGIN      
        1 cattccgccg ccggcaatgg cgacgttcga cgcgccgacc cgcgaattct gctcggtgcg
       61 cgaatcgcgc accaatacgc cgctgcaagc cttgctcatg ctcaacgagc cgacgctcgt
      121 cgccgcggca caggcgctgg ccgaccgcgt aatgcgggag gcgacagcgc cggacgatcg
      181 cgtcacgcgg gccatgctct gcgcggtcgg gcggccgctg acggagcaag aggccgcgat
      241 tttccgggcg gccctggcgc gctatcgcga tcgcggcgct gacgtagctg cggcgtacgc
      301 tctcctgtgc ggcacgatcc tgaacctcga cgaagcggtg acgcggcaat gagcaacatc
      361 ggtccaacgc cgtttccagt gacgcgccgc gacctgctgg gccagatggc cacgggcatc
      421 ggcgcggcgg cgctcggcac gctgctgtcc ggcgaatcgc gcggcgaaga atcgctgccg
      481 aaaccgcatt ttgcccccaa ggccaagcgc gtcatcttcc tgttccagtc cggagcgccg
      541 tcgcagctgg acctgttcga tcccaagccg aaactggccg acctgcgcgg tacggagctg
      601 cccgactcga tccgccaagg gcagcggctg accggcatga cctcgcggca gagcagtttt
      661 ccggtggccc ccagccgctt tggcttcgcc cagcacggca agagcggcgc gtggctcagc
      721 gacctcttgc cgcacaccgc cggagtcgcc gacgagctgt gcttcgtgaa gtcgctgttc
      781 accgaggcga tcaaccacga cccggcaatc accttttgcc agactggcgc tcagctggcc
      841 ggccggccga gcatcggctc ttggctggcg tacggcctgg gaagcgaaaa cgaaaacctc
      901 ccggccttcg tcgtgctcgt cagccagggc accggcaaca ccggcgatca gccgctctac
      961 gaccggctgt ggggcagcgg gttcatgccc tcgcggtacc agggcgtcaa gttccgctcg
     1021 accggcgacc cggtgctgta cctgtcgaat cccgaaggcg tagatagcac ggcccgccgg
     1081 gcgatgctcg acgacgtggc cgccctcaac cggctgaacc agcagcaatt cggcgacccg
     1141 gagattgcga cccgcatcgc ccagtacgag ctggcgttcc ggatgcagtc cagcgtgccg
     1201 gagctgctcg acatggccgg cgaggacgcg cagacgctgg agcggtacgg accggaagtc
     1261 cgccgccgcg gcagctatgc ctataactgc ctgctggccc ggcggatggc cgagcgcggc
     1321 gtgcggttcg tgcagctctt tcaccgcggc tgggaccagc acgtctttct gcccaagcag
     1381 atcgccgccc agtgccgtga taccgatcag ccgacggccg cgctcatcgc cgatctcaag
     1441 cagcggggcc tcttggacga cacgctggtc gtgtggggcg gcgagtttgg ccgcacggtc
     1501 tattgccagg gcacgctcac ggccgacgac tatggccgcg accatcatcc gcggtgcttc
     1561 accgtctggc tggctggcgc cggcgtcaag agcgggacca gcttcggcca gaccgacgac
     1621 ttttcctaca acatcgcggc cgaggcggtt cacgttcacg acctgcacgc cacgatcctg
     1681 cacttgctgg gcatcgacca cacgcggctg acgtaccgct tccagggccg ggacttccgg
     1741 ctcaccgacg tcgcggggaa cgtggtgcgg ccggtcctgg cctgagggac gtaccgccgg
     1801 ctgatttctg ggcggggagg cattgcgggc ggcctctcat ctcgtgaggc ggcccgccgg
     1861 agcggccgca gccaggcagg agagaacgcc gatgcagccc aacgagtgga acgccgccga
     1921 cgcgattttc cggcgctacc tcgagcgccc ccggcgccgc cgactcgctc gagtcgtgga
     1981 cgctttctac cagcatgttt gggccacggc ttaccgtgtc acaggcaatg ccgcggatgc
     2041 cgcggacatc acgcaggacg tctttctcca gttgctcctc cagccgccgc ggccggagag
     2101 cgtgaggtgt ccccgcgcct ttctggcatg gctggtggtc ggtcgagctt cgggccttcg
     2161 ccgatcggcc aagcggagga aagcccggga gcaatcggcc ttgcgacagg cagcggacga
     2221 agggatcgtc agtgcggaca tcgaagctct gcgagattgc gtggaggatc ttcccgaaga
     2281 tctccgcgcg gccgtcgcgc tccgctattt tgccggcctc aagaatagcg atgtcgccct
     2341 ggccacgggg gtgggggagc ggcaagtgga gaagcgcctc gaagaggcgc gtgccatcct
     2401 gcgggcccgg cttggaccca cagcggcacc ggtcctgctc
//
LOCUS       DASRJB010000412         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_114248, whole genome shotgun sequence.
ACCESSION   DASRJB010000412 DASRJB010000000
VERSION     DASRJB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114248"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1178
                     /locus_tag="VFB80_10910"
     CDS             135..1178
                     /locus_tag="VFB80_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN34323.1"
                     /translation="MSISRRRFVLSCASCAAAGWLARIPLAAADETRAAGWPSRVVQL
                     PQGEDELKPPVVTAVRLHKDGQLLATAGDDHLVRVWNLAEGKLVQKLSGHADWVRTLD
                     YSPDGRVLATAGNDRKVIFWDAASGSQLESFAEHPAAIAAVRFSHDGRLLAAAGFEGL
                     VRIYDVAARRLVAQPAAPCNDMRSLAFSPDDGLLAAGGRCGTLRLLAMPRGEVDRDIR
                     AHRLRIRTIAFSPDGAYVASSGEDRLIHVTPLADAAGGYSLPPRPAKVLSLVFYGPRQ
                     LAAAGSDNLIRLWDVAARGEIGHLRGHTGSVAALGCQGKVLVSAGYDTTVRIWEVGDQ
                     IAEASPPGSGTLR"
     gene            1271..5365
                     /locus_tag="VFB80_10915"
     CDS             1271..5365
                     /locus_tag="VFB80_10915"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HZN34324.1"
                     /translation="MGLWSLLSGWRKLPREKPAAASRACRFETMEERRLLDADPIKIG
                     VTYLEEDSGSDLHGDTFQVLFEGGAAGTELTRLVIDGDHGPAGLSFADMIFDTVKGGL
                     GADEAFNLQVISSTGIQHVSWQVSDGGSKLTLLFSGFQAGEKLVFSIDVDEVQQFDPS
                     QTNQQLINEGIDPIASGVEFQSSHLTADFHAPHYHDIGGTSEFRNLYDAQFSGAGLLV
                     TQGNPSGLPNDDFNGKRDRSTGTMLQLQQLPLPVTIAGRVFADNNRNLVQDGSDFGMA
                     GVSLALWKQVNGQWTNTGHTTTTNVQGDYSFGLNLNLQPGVYQVRETQPAGFFSVGAI
                     PGTVGGAPTGATIAGDPDVLTEINLPLGDLHGIHYNFAEAQPASLSGRVHLTDRQGNC
                     FSEEALNRPLAGVKITLKDAAGNVVATQLTNASGEYLFQNLLPGTYTIVEETPAGLLD
                     GADHIGTIGGIKVGAKPANDTISQIILLGGQDGVNYDFCEHEPADVGGFVYHDANNNG
                     VKDASETAIGGATVVLLDAGGTQVATTTTDSSGFYKFANLAAGTYSIREVQPSGWLDG
                     QDAAGTIAGQIVGSAENPGDRINGVALLWGDSGVNYNFGELLPGSLSGRVWVDPNEND
                     VFDAGDSPLAGVTIQLVNQSGQAVGTTTTDAQGQYEFTDLAPGVYAVRELQPGGYLQG
                     GQRAGSGGGNDSATDLISQITVGSGDHFVNYDFWELQSVSIAGHVWADTIPNCIFDPS
                     ESAILGVTIQLLNSQGQLVATTQTLADGSYLFANLRPGTYSVRELQPAGYFDGEDMPG
                     NLGGDASQNDLIASVVLKSGDVGVNYDFCETPPAKISGYVFRDGPPITSPTGEIPENL
                     YELRDGLLTPDDLRLRGVVLELRYTLTGEPVRGEDLLPGKWLPGPVRVVTGADGYYEF
                     DGLPQGNYSIFELHPSGFIDSRDTAGTTSGLAINVGTLVSPLVVQTFAAQGVSFKFDA
                     IVQVPLAAGQHSQFNNFSEVQVKPVLILIPPPPPVEPPPEPPLFLQQQLLAPPPDIFI
                     PPPVAAPIVTGGTGNFTWHLSIIDAGLPRIARRSTRHADLVFRPALWVERTEWLAENL
                     RAGTWTIHTADDPLNTAMFGLPGATPVVGDFNGDGKDEIGVYYEGEWFLDLNGNGHWD
                     PADLWAKLGSKADKPVIGDWDGDGKDDIGIYGPEWAGDPVQIEHEPGLPDQDNQRRRE
                     QKPKNVPPNPEEATDGERLLRLTAQGKERADLIDHVFAFGGRNDHPIAGDWNGDGIRS
                     IGVFREGKWHFDMDGDGRWSDGDKLAKFGQKGDLPVVGDFNGDGIEEIAVFRGGRWII
                     DTNGNRQIDAADRTLDLGQEGDVPLAGDFNGDGIDEPAVYRPSK"
     gene            5537..5980
                     /locus_tag="VFB80_10920"
     CDS             5537..5980
                     /locus_tag="VFB80_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN34325.1"
                     /translation="MEEFDKSIDTIVRDCIAVRVRLLNRAVTGLYDDALRPLGLKVSQ
                     LNILVATAKLGVARPAMVCEVLHMDTSTLSRNVERMRKNGWIETVAGDDGRMQPFQLT
                     GRGKRLLERAFPVWREAQREALELLGENGAAVLSKIAGRLGMRVK"
     gene            6019..>6328
                     /locus_tag="VFB80_10925"
     CDS             6019..>6328
                     /locus_tag="VFB80_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34326.1"
                     /translation="MKLADHPTVKAVESKAATPPPTVVDAQWLRQLCREAGADDVGLV
                     DIGRPALDDQRSEILSFFPPAKTLVSFVCRMNREPIRNPARSVANLEFHHAGDKVNDV
                     A"
BASE COUNT         1159 a         2145 c         2022 g         1002 t
ORIGIN      
        1 cctgtccgca cagcttcacg cgcttttgtc cgaagaatcg ttgaaattgg acctgccgcg
       61 ccgcgcgggc cgatagatga cgaggagcgg tggcttgcgt caccgcgact cccgccactt
      121 ttcgcccgcc cgccatgtcg atctcccgcc gccgcttcgt cctatcttgc gcgtcgtgcg
      181 ccgcggccgg ctggctggct cgaatccccc tcgccgccgc cgacgagacc cgcgccgcag
      241 gctggccctc gcgcgtcgtg cagctgccgc agggcgagga cgaattgaag ccgccggtgg
      301 tcacggcagt gcgattgcac aaggacgggc agctgctggc tacggccggc gacgaccatc
      361 tggtccgcgt ctggaatttg gccgaaggca agctggtgca aaaactgagc ggccacgccg
      421 actgggtccg cacgctcgac tattcgcccg atggccgcgt cctggcgacc gcgggcaacg
      481 accgcaaagt catcttctgg gacgcggcca gcggctcgca gctggagtcg tttgccgagc
      541 atcccgccgc gatcgccgcg gtccgcttca gccacgacgg gcggctgttg gcggctgccg
      601 gcttcgaagg cctggtgcga atctacgacg tcgccgctcg ccggctcgtg gcccagccgg
      661 ccgcgccgtg caacgacatg cggtcgttgg ccttctcgcc cgacgacggc ctgctggccg
      721 ccggcggtcg ctgcggcacg ctgcggctgc tcgcgatgcc acggggcgaa gtggaccgcg
      781 atatccgggc ccatcggctg cgaatccgca cgattgcgtt ctcgcccgac ggagcgtacg
      841 tcgcgtcgtc gggcgaggac cgcctgattc acgtgacgcc gctggccgac gccgccggcg
      901 gatacagcct gccgccgcgg ccggccaagg ttctctcgct ggtgttctac gggccgcgtc
      961 agctggcggc cgccggaagc gacaacctga ttcgcctgtg ggatgtcgct gctcgcggcg
     1021 aaatcggtca cctgcgcggc cacacgggca gcgtcgccgc cctcggctgc cagggaaaag
     1081 tgctggtcag cgctggctac gacacgaccg tccgcatctg ggaagtcggc gaccagattg
     1141 cggaagcaag tccgcctgga agtggaacgc tgaggtgaag gaagggctgg ggctaggggc
     1201 tagggctagg gactgcaatt tctctcccta gccctagccc tagccccagc ccctagcacg
     1261 gaggtgcctt gtgggattgt ggagcctgct ctctggttgg cgaaagctgc cccgcgaaaa
     1321 gccggcggct gccagccggg cgtgccgctt cgaaacgatg gaagagcggc ggctcctgga
     1381 tgccgacccg attaagatcg gcgtcacgta cctggaagaa gacagcggca gcgacctgca
     1441 cggcgacacc tttcaagtcc tgttcgaagg gggcgccgct ggcaccgagt tgacccggct
     1501 ggtaatcgac ggcgaccacg gcccagctgg cctctcgttc gccgacatga tcttcgacac
     1561 agtgaagggc ggcctgggcg ccgacgaagc attcaatttg caggtcatca gcagcacggg
     1621 aattcagcac gtcagctggc aggtctccga cggcggctcg aagctcacgc tgctcttcag
     1681 cggcttccag gccggtgaga agctggtctt ttcgatcgac gtcgatgaag tgcagcagtt
     1741 cgaccctagc cagaccaatc agcagctcat caacgagggc atcgacccga ttgcctcggg
     1801 cgtcgagttc cagagttcgc acctgaccgc cgattttcac gcgccgcact atcacgatat
     1861 cggcggcacg agcgagtttc gcaacctcta cgacgcccag ttcagcggcg cgggcctgct
     1921 cgtcacgcaa ggcaatccaa gcggcctgcc caacgacgac ttcaacggca agcgcgaccg
     1981 ctcgaccggc acgatgctcc agctgcagca gctgccgctg ccggtgacga tcgccggccg
     2041 cgtctttgcc gacaacaacc gcaacctcgt gcaggacggc agcgacttcg gaatggccgg
     2101 cgtctcgctc gccctatgga agcaggtcaa cggccagtgg acgaacacgg gccacaccac
     2161 gacgaccaac gtccagggcg attattcgtt cgggctgaat cttaatctgc agccgggcgt
     2221 ctatcaggtc cgcgagacgc agccggcggg cttcttcagc gtcggagcga tcccgggcac
     2281 agtcggcggc gcgccgactg gcgctacgat cgccggcgat cccgacgtgc tgaccgagat
     2341 caacctgccg ctgggggacc tgcacggcat tcactacaac ttcgccgagg cccagccggc
     2401 atcgctgagc ggccgcgtcc atctgaccga ccgccagggc aactgtttta gcgaagaagc
     2461 tctcaaccgg ccgctggccg gcgtgaagat cacgctcaag gacgcggcgg gcaacgtcgt
     2521 cgccacgcag ctgaccaatg ctagcggcga gtatttgttc cagaacctgc tgccgggcac
     2581 gtacacgatc gtcgaagaga cgccggccgg actgctcgat ggggcggacc atatcggcac
     2641 gatcggcggc atcaaagtcg gggcgaaacc ggcgaacgac acaatcagcc agatcatcct
     2701 gctcggcggc caggacggcg tgaactacga cttctgcgag cacgagccgg ccgacgtcgg
     2761 cggcttcgtc tatcacgacg ccaacaacaa cggggtcaag gacgccagcg aaacggccat
     2821 tggcggcgcg accgtcgtcc tgctcgacgc cggcggcacg caagtcgcca ccacgaccac
     2881 cgattccagc ggcttctaca agttcgccaa cctcgcggcc ggcacgtact cgatccgcga
     2941 agttcagccc agcggctggc tcgacggcca ggacgccgcc ggcacgatcg ccgggcaaat
     3001 tgtcggctcg gccgagaatc ccggcgacag gatcaacggc gtcgcgctgc tctggggcga
     3061 cagcggcgtg aactacaact tcggcgagct attacccggc agcttgagcg gccgtgtctg
     3121 ggtcgatccc aacgagaacg acgtcttcga cgccggcgac tcgccgctgg cgggcgtcac
     3181 catccagctc gtcaaccaga gcggccaggc cgtcggtaca accaccaccg acgcccaggg
     3241 gcagtacgaa ttcaccgacc tggcccccgg cgtctatgcc gtccgcgaac tgcagcctgg
     3301 cggctacctc cagggcggcc agcgagccgg cagcggcggc ggcaacgaca gtgcgaccga
     3361 cttgatctcg cagattaccg tcggctcggg cgatcacttc gtcaattacg acttctggga
     3421 gctgcaatcg gtcagcatcg ccggtcacgt ctgggccgat accattccca actgcatctt
     3481 cgatcccagc gaatcggcaa tcctcggcgt gacgatccag ctgctcaata gccagggaca
     3541 gctcgtcgcg acgacccaaa ctctcgccga cggcagctac ctgttcgcta atcttcgccc
     3601 cggcacttac agcgtccgcg agctgcagcc cgccggctac ttcgatggcg aagacatgcc
     3661 gggcaacctg ggcggcgatg cctcgcagaa cgacctgatc gcgagcgtcg tcctcaagtc
     3721 cggcgacgtc ggcgtcaatt acgacttctg cgaaacgccc cccgcgaaaa tctcgggcta
     3781 cgttttccgc gacggcccgc ccatcacctc gccgaccggc gagatccccg aaaacctcta
     3841 cgagctccgc gacggcctgc tcacgcccga cgacctgcgg ctgcggggcg tcgtgctcga
     3901 gctgcgctac acgctcaccg gcgaaccggt gcgaggcgaa gacctgctgc ccggcaagtg
     3961 gctgccggga ccggtccgcg tcgtgacggg cgccgacggc tactacgaat tcgacggcct
     4021 gccgcagggc aactactcga tcttcgaact ccaccccagc ggcttcatcg acagccgcga
     4081 cactgccggc accaccagcg gcctggcaat caacgtcggg acgctggtca gcccgctggt
     4141 cgtgcaaacc ttcgccgcgc agggcgtgag tttcaagttc gacgcgattg tgcaggtgcc
     4201 gctggccgcc gggcagcatt cgcagttcaa caacttcagc gaagtgcagg tcaagccggt
     4261 gctgatcctc attccgccgc cgccgccggt cgagccgccg cccgagccgc cgctgtttct
     4321 ccagcagcaa ctgctcgcgc cgccgccgga catcttcatc ccgccgccgg tcgccgcgcc
     4381 gatcgtcacc ggaggcacgg gcaacttcac ctggcacctg tcgatcatcg acgcgggcct
     4441 gccgcggatc gcccgccgca gcacgcgaca tgcggacctc gtcttccggc cggcgctgtg
     4501 ggtcgagcgg accgagtggc tcgccgagaa cctgcgggcc gggacgtgga ccatccatac
     4561 cgccgacgat ccgctcaaca cggcgatgtt cggcctgccc ggcgcgacgc cggtggtcgg
     4621 cgacttcaac ggcgacggca aggacgagat cggcgtctat tacgagggcg agtggttcct
     4681 cgatctcaac ggcaacggtc actgggatcc ggccgacctg tgggccaagc tcggcagcaa
     4741 ggccgacaag ccggtcatcg gcgactggga cggcgacggc aaggacgaca tcggcatcta
     4801 tggccccgag tgggccggcg accccgtgca aatcgagcac gagccgggcc tgcccgatca
     4861 ggacaaccag cggcgccgcg agcagaagcc caaaaacgtg ccccccaatc ccgaagaggc
     4921 gaccgacggc gagcgactgc tgcggctgac cgctcagggc aaggagcggg ccgacctgat
     4981 cgaccacgtc tttgcgttcg gcggccggaa cgatcatccg atcgcgggcg actggaacgg
     5041 cgacggcatc cgctcgatcg gcgtcttccg cgaaggcaag tggcatttcg acatggacgg
     5101 cgacggccgc tggtcggacg gcgacaagct cgccaaattc ggccagaagg gcgacctgcc
     5161 ggtcgtgggc gacttcaacg gcgacggcat cgaagaaatc gccgtcttcc gcggcggacg
     5221 ctggatcatc gacaccaacg gcaaccgcca gatcgacgcc gccgatcgca cgctcgacct
     5281 cggccaggaa ggcgacgtgc cgctcgccgg cgacttcaac ggcgacggca tcgacgaacc
     5341 agctgtctat cggcccagca agtagcgggc gctcatttct tctgcagcag ctaaagaccc
     5401 ggttcccagg cttcagcctg ggaacccact tccccgaggc tcccgcctcg tttcacaccc
     5461 agcgatccgg aacgaccttg attacgccac ttgacgaagc cagtagttgc atatacaaac
     5521 aatgcaggcg ggaacgatgg aggaattcga caagtcaatc gacacgattg tccgcgattg
     5581 cattgcggtg cgggtgcgcc tgctcaaccg ggcggttacc ggtttgtacg acgatgcgct
     5641 gcggccgctc ggactgaaag taagccaatt aaacattttg gtggcgacgg ccaagctggg
     5701 cgtggcccgt cccgcgatgg tttgcgaggt gctgcacatg gacacgtcca ccctcagccg
     5761 taacgtcgaa agaatgcgca aaaacggctg gatcgagacg gtggcgggtg atgacggtcg
     5821 gatgcagccg ttccagttga cgggcagggg aaagcggctg ctggaacgcg cctttccagt
     5881 ctggcgtgag gctcagcgcg aggccctcga acttctaggt gaaaacggcg cagccgtgct
     5941 ctcgaaaatc gcggggaggt tgggaatgcg ggtcaaataa ttttttgtcc acatatttgc
     6001 atatacaagg agccgcccat gaaactggcc gatcatccca cggtcaaggc tgtcgaaagc
     6061 aaggcagcga ctccgcctcc gacggttgtc gatgcgcagt ggttacggca actgtgccgg
     6121 gaggctggcg ccgacgatgt cgggctggtg gatattggcc gcccggcgct tgatgaccag
     6181 cggagtgaaa ttctgagttt ctttccaccg gcgaagaccc tcgtcagctt cgtttgccgg
     6241 atgaatcgtg aaccgattcg caatccggcc cgctcggtgg ccaacctgga atttcaccac
     6301 gccggcgaca aggtaaacga cgtcgccc
//
LOCUS       DASRJB010000413         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_965405, whole genome shotgun sequence.
ACCESSION   DASRJB010000413 DASRJB010000000
VERSION     DASRJB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965405"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4033)
                     /locus_tag="VFB80_10930"
     CDS             complement(<1..4033)
                     /locus_tag="VFB80_10930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN34327.1"
                     /translation="MALDLFHPLIRQWFAGRFGQPTEPQLRGWPEIAAGKHTLIAAPT
                     GSGKTLAAFLVCLDRLLRKWLEGKLPEGIEVVYVSPLKALSNDIQRNLETPLAEIAAL
                     AADAGLGELPIRTAVRTGDTPQAERAAMLRRPPHILVTTPESLYLLLTSAKSREKLRT
                     VNTVIVDEIHALARDKRGSHLSLSLERLSALCEQPPVRIGLSATQKPIEEIGAFLVGQ
                     EFKVQGSKFKVEEPKPTLNLELGTLNSAAPAVVNIGHARDLDLGLELPQLELAAVCTH
                     EHWGEVYQRLTELIQSHRSTLVFVNTRRLAERVAFRLTEQLGEEAVASHHGSLSREIR
                     LAAEQRLKAGKLKAIVATASLEMGIDIGYIDLVCQIGSPRAIATFLQRVGRSGHAVGA
                     LPKGRLFPLTRDELLECLALVRSVRRGILDRIEIPQAPLDILAQQIVAAVAADEWDED
                     ALFRLCRGAYPYRNLDREKFDAIVKMLSEGIRKGNRAGTWLHRDLINGRLRARRGARL
                     AAVTSGGAIPEAAVYRVITEGEGTFVGTVDEDFAVESLAGDVFLLGNTSWQIRAVRGA
                     EVIVTDAHGAPPTIPFWLGEAPGRTVELSHEVSELRRELAERVQGSEFMVQGQESGVG
                     GRESEEPAFCRVYERQSGTGSRRQTAPNLQSEIGKLQSLPTPDPRLPTPDPLAWLQSE
                     TGCGPWAAQQAAQYVAAQKAAIGCVPTAEEVVFERFFDESGGMQLVVHAPLGSRINKA
                     WGLALRKRFCRSFDFELQAAADDNGVLLSIGPQHSFPIDSLFGMISPANGRDLLEQAV
                     IAVPMFQVRWRWNITRALAVLRQQGGKRVPPFLQKFRSEDLLAAAFPETVGCLENHHG
                     PVVIPDQPLVQQTMHDCLTEAMDIERWLAFLGEVQSGNVRFIPVETREPSPFSHQLLN
                     ANPYAFLDGAPLEERRTRAVTTRRSLAIEDVRDLAKLDPAAIAQVAAEAWPLVRDADE
                     LHDALMNFVVLPENEGREWGGYFEQLVRAGRAAVATIAGGARFWIAAERWPLVRAVYK
                     DASVQPPLELPASLTSQEWSASDGWVEIVRGQIQCRGPHTAEELADLLRLEPSQVFAA
                     LESLEGSGIAMRGQFRNQETGDRGQETVGAGGNALRGVPSAVEWCERRLLARIHRQTL
                     DGLRRRIEPVAPEVYWQYLVEHHHLTENRQLQGELGLREAIAQLEGFELPAGVWESKV
                     LAPRLADYDPQWLDHLFLSGELVWGRLRPPKRDEEDGPGMAALTRSMPITLALREDLP
                     WLLPEERPDVSPFAGGAAQEVLNALRQRGALFFQDLKSLTGLLPSQLEEALRELAALG
                     LVSSDTFAAVRAIDGGGAAKAATL"
     gene            complement(4053..>4516)
                     /locus_tag="VFB80_10935"
     CDS             complement(4053..>4516)
                     /locus_tag="VFB80_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN34328.1"
                     /translation="SVLLGLMIYEWTRYCGTEIESGRDMTCDREDMKQGLEPDLCYWT
                     INQPLVYGKDEVDFSVDPPPDLALEVDITRSSIPKLPIYEALGVPEVWCWRNGIEVLA
                     LRDGHYARVSESVVLPGFPLRLAEDFIQRRNSAGENALMAQFVAALANLPR"
BASE COUNT          742 a         1538 c         1484 g          752 t
ORIGIN      
        1 ccagcgtggc cgccttcgcc gcgccgccac cgtcgatcgc ccgcacggcg gcaaacgtgt
       61 cggacgagac gagccccagc gccgccagct cgcggagcgc ctcttcgagc tgcgacggca
      121 agagcccggt caagctcttc aggtcctgga agaacagggc cccgcgctgg cggagggcat
      181 tcaagacttc ctgggccgcc ccgccggcaa agggcgagac atccggccgc tcctctggca
      241 acagccacgg caggtcttcg cggagggcga gcgtgatcgg catgctccgc gtgagcgcgg
      301 ccatgccggg gccgtcttct tcgtcccgct tgggcggccg cagccggccc cagaccagct
      361 cgcccgacag gaacaaatgg tcgagccact gcgggtcgta gtcggccagc cgcggggcga
      421 gcaccttgct ctcccacacg cccgccggca attcgaaccc ttccagttgg gcaatcgcct
      481 cgcggaggcc cagctcgccc tgcaattggc ggttctcggt caggtggtgg tgttcgacga
      541 ggtattgcca atagacctcc ggcgcaaccg gctcgatccg ccgccgcagg ccgtcgaggg
      601 tctgccggtg gatgcgggcc agcaagcggc gctcgcacca ttcgaccgcc gagggaacgc
      661 cacggagggc gttccctcca gcgccgactg tctcctgtcc cctgtctcct gtctcctgat
      721 tcctgaactg cccgcgcatg gcgatgccgg aaccctccaa ggattcgagt gccgcaaaca
      781 cctgcgaagg ttccagccgc aacaggtccg ccaactcttc cgccgtgtgt ggcccgcggc
      841 actggatctg cccgcggaca atctcgaccc agccgtcgga tgcggaccac tcctggctcg
      901 tcagtgacgc gggcaattcg agcggcggct gcacgctcgc gtccttgtag accgcccgca
      961 ccagcggcca ccgctcggca gcgatccaaa atcgcgcgcc gccggcgatc gttgccaccg
     1021 ccgctcgtcc tgcacgaacg agctgctcga agtagccgcc ccactcgcgg ccttcgttct
     1081 ccggcaggac gacgaagttc attagcgcat cgtgcagctc gtcggcgtcg cggaccagcg
     1141 gccaggcctc ggcggcgact tgcgcgatgg cggccgggtc gagcttcgcc aggtcgcgga
     1201 cgtcctcgat cgccagcgac cgccgcgtcg tgaccgcccg cgtgcggcgc tcttcgagcg
     1261 gcgcgccgtc gagaaacgcg tacgggttgg cgttcagcag ctggtggctg aacggcgacg
     1321 gctcgcgggt ttcgaccgga atgaaccgca cgttcccgct ctgcacttcg cccaggaacg
     1381 ccagccagcg ctcgatgtcc atggcctcgg tcaggcagtc gtgcatcgtc tgctgcacca
     1441 atggttgatc gggaatcacg accggcccgt ggtgattttc caggcagccg acggtttcgg
     1501 ggaaagccgc ggccaagagg tcctcgctgc ggaacttctg caggaagggc ggtacgcgct
     1561 tgccgccttg ctgccgcagg acggcgagcg cgcgggtgat gttccaccgc cagcggacct
     1621 ggaacatcgg cacggcgatc accgcctgtt cgagcaggtc gcggccattg gccgggctga
     1681 tcatgccgaa caggctgtcg atcggaaagc tgtgctgcgg gccgatcgag agcaggacgc
     1741 cgttgtcgtc ggccgccgcc tgcaactcga agtcgaagct gcggcagaac cgcttgcgga
     1801 gggccaggcc ccaggccttg ttgatccgcg agccgagcgg cgcgtggacc accagctgca
     1861 tcccgcccga ctcgtcgaag aaccgctcga agacgacttc ctccgccgtc ggcacgcagc
     1921 cgatcgccgc cttctgagcc gcgacgtact gcgccgcctg ctgcgccgcc caagggccgc
     1981 agccggtttc ggattggagc caggcgaggg ggtcgggggt cgggagtcgg gggtcgggag
     2041 tcggcagcga ttgcagtttg ccgatttcag attgcagatt gggggcggtt tgccgacggc
     2101 ttccagtacc gctctgccgt tcgtagactc ggcaaaatgc tggctcttcc gactcccgac
     2161 ccccaactcc cgactcctga ccctgaacca tgaactccga accctgaacc ctctccgcca
     2221 gctcccgtcg cagctcggag acttcatgcg acaactcgac ggttcggccg ggggcctcgc
     2281 cgagccagaa ggggatggtc ggcggagcgc cgtgggcgtc cgtgacgatg acctcggcgc
     2341 cgcgaacggc gcggatttgc cacgaggtgt tgcccagcag gaagacgtcg ccggcgaggc
     2401 tctcgacggc aaaatcctca tcgacggtgc cgacgaacgt gccttcgcct tcggtgatga
     2461 cgcgatagac ggctgcctcg ggtatcgcgc cgccggaggt gaccgcggcc agccgcgcgc
     2521 cgcggcgggc ccgcaggcgg ccgttgatca ggtcgcggtg cagccaggtc ccggcgcggt
     2581 tgcctttcct gatcccctcc gagagcatct tcacgatggc gtcgaatttt tcacgatcca
     2641 ggttccgata gggataggcc ccgcgacaga gccgaaatag cgcgtcctca tcccattcgt
     2701 cggcggcgac tgcggccacg atctgctgcg cgaggatatc gagcggcgct tgcggaatct
     2761 cgatgcggtc caggatgccg cggcggaccg agcggacgag ggccaggcac tccaagagct
     2821 cgtcgcgcgt cagcgggaag aggcggccct tgggcagggc ccccaccgcg tggcccgagc
     2881 ggccgactcg ctgcaaaaac gtggcgatgg cccgcggcga gccgatctgg cagaccaggt
     2941 cgatgtagcc gatgtcgatt cccatctcga gcgaggccgt ggccacgatc gccttgagct
     3001 tgccagcctt cagccgctgc tcggcagcga ggcgaatctc gcgcgacagg ctgccgtggt
     3061 ggctggccac cgcctcttcg ccgagctgct cggtcaggcg aaacgccact cgctccgcca
     3121 accgccgcgt gttcacgaac accagcgtgc tgcggtgcga ctggatcagc tccgtcagcc
     3181 gctgatagac ctcgccccaa tgctcgtgcg tgcaaaccgc cgccagctcc aattgaggca
     3241 actccaggcc caggtccaga tcgcgagcgt ggccgatgtt gacgaccgcc ggggcggcgg
     3301 agttcaaagt tccgagttca aggttcaagg ttggcttcgg ctcttcaacc ttgaactttg
     3361 aaccttgaac cttgaactcc tgcccgacca gaaacgctcc gatttcctcg atcggcttct
     3421 gcgtcgccga gaggccgatg cgaacgggcg gctgttcgca gagtgcgctg agccgttcca
     3481 gcgagagcga caggtgcgag ccgcgcttgt cgcgggccag ggcgtgaatc tcatcgacga
     3541 tgaccgtatt caccgtccgc agcttctcgc ggctcttggc cgaggtcagc agcaggtaaa
     3601 gcgattcggg cgtcgtaacc aggatgtgcg gcgggcggcg gagcatggcc gcgcgttcgg
     3661 cctgcggcgt gtcgcccgtg cggaccgccg tgcggatcgg cagttcgccc aagcccgcgt
     3721 cggccgccag cgcggcgatc tcggccagcg gcgtttccag gttccgctga atatcgttgc
     3781 tcaaggcctt cagcggcgag acgtagacga cttcgattcc ctccggcagt ttcccctcga
     3841 gccattttct gagcagccgg tcgaggcaga ccagaaacgc cgccaaggtc ttgcccgacc
     3901 cggtcggcgc ggcgatcagc gtgtgcttgc cggccgcgat ttccggccag ccgcggagtt
     3961 gcggctccgt cggctggccg aatcggccgg caaaccactg gcggatcagc ggatggaaga
     4021 ggtcgagcgc catacgtccg tgcgtttcgt agctatcgag gcagattggc gagagccgca
     4081 acaaactgcg ccatcagggc gttttcgcca gcggagtttc gccgctggat gaagtcttcg
     4141 gccaggcgca atggaaaccc aggcaagacg acgctttcgg agacgcgggc gtagtgtcca
     4201 tcgcgaagtg ccaagacctc gatcccattc cgccagcacc acacctccgg cacccctaag
     4261 gcctcataga ttggcagctt ggggattgac gaccgtgtga tatcgacctc cagcgccaga
     4321 tccggaggcg gatcgacgga gaaatcgact tcgtccttgc catataccag cggctgattg
     4381 atcgtccagt agcacagatc gggctccaat ccctgtttca tgtcttcgcg gtcgcaggtc
     4441 atgtcgcggc ccgattcgat ttcggtcccg cagtaacgcg tccattcata gatcatgaga
     4501 cccaggagaa cggaga
//
LOCUS       DASRJB010000414         7985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1525802, whole genome shotgun sequence.
ACCESSION   DASRJB010000414 DASRJB010000000
VERSION     DASRJB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525802"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFB80_10940"
     CDS             <1..606
                     /locus_tag="VFB80_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN34329.1"
                     /translation="QEREVSIGRQTFELPDPFFTIATQNPIEQEGTYPLPEAQLDRFM
                     FNIKVGYPTADEEEKILTQTTRTEKIEVRKILSSVAIVRLQKLVHSVAVSEYIVKYVS
                     RLVRATRPKDETAPQFVKDLVDWGAGPRAGQFLINGGKALAAMDGRFSVAIDDVKRIA
                     IPVLRHRLSTNFQAQAEGMSTEDVIQRLLKEVKEPEVAKFA"
     gene            complement(633..1019)
                     /locus_tag="VFB80_10945"
     CDS             complement(633..1019)
                     /locus_tag="VFB80_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZN34330.1"
                     /translation="MAISSFKDLIVWQKSIELIEEVYRLSATFPKQETYGLSSQLQRA
                     AVSIAANIAEGSGRHSTKEYVHHLSYSLGSLAEVETYFVVCVKLGYATQKSVSSLETK
                     ADQIGKMLRSLQKALRRKLGPDDECP"
     gene            1120..2016
                     /locus_tag="VFB80_10950"
     CDS             1120..2016
                     /locus_tag="VFB80_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN34331.1"
                     /translation="MASVEKYLKPEVINQIKRLDLRAQFVVKGFLQGLHASPFHGFSV
                     EFSEHRRYTHGDDPKDIDWLVFAKTDKYYVKKFEAETNITGYLVMDLSQSMGFTHRQE
                     LTKFDYAICLAASLCYLMIYQQDPVGLVTFDQKLRDVIPPKSKRTQLGNILAHLANLK
                     PAGQTDIAKSITQLAAMLRHKSLIMLFSDLLQDPGPVFQALRRLRHRGHDVILFHILD
                     EAEVHFPYQGMIEFEDPETQEKVVVDASSYRRDYQTEIEAFRGLFRQECFQTGVDYVA
                     LDTSLQFDKALLEYLSSRRARG"
     gene            2090..3466
                     /locus_tag="VFB80_10955"
     CDS             2090..3466
                     /locus_tag="VFB80_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34332.1"
                     /translation="MPRLSLAALCLASLPLVATAADKPMNFVVVLADDIGAKELACYG
                     NTQHQTPRLDRLAKEGMRFETCWVTPLCTPTRVLTMTGQYAFRTGYFQMFGAALSPRP
                     NSPEYQIGDKFTFADMLKAKGYATGMCGKWQLSGEHPTLIRDCGFDEYRMWAYEHNLP
                     AGVTHTGRYEGGNRTARYWHPSIVENGKYLTTQPNDYGPDLFNAFAIDFITRHKAGPF
                     CFYYTMPLTHGPHEETPDPAHPGQRRPAGYKSNVEYLDYLMGNLLDAIDKLGIAENTL
                     FIFIGDNGTAGSGKGTVTELGVRVPLIVRCPGTVKAGVVSRELASGADVFATLQEFSG
                     AALPAGHVIDGKSLGHTLRGEGGQHREWLFSYLGPGRILRDERFILEVPGQGQPQKLI
                     DCGQSRNGEGYREVTQPTPESTAALARFAKLLADLPGPENHPGLRLPAAGEEGKAKTK
                     AKAKKKKA"
     gene            complement(3475..3870)
                     /locus_tag="VFB80_10960"
     CDS             complement(3475..3870)
                     /locus_tag="VFB80_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZN34333.1"
                     /translation="MDNPDFMQTVQAWANQLLIWVGFGTIVGLLAKAIMPGKDPGGAI
                     ATLAMGVAGTIIGCGVLSYFWRGHVISPISPLGFCVATAGAFVLLFFYKLLGGYYFEE
                     DQGPLVRRRRFGLRRRTRPPAAVVYDDET"
     gene            complement(4689..5702)
                     /gene="hpnH"
                     /locus_tag="VFB80_10965"
     CDS             complement(4689..5702)
                     /gene="hpnH"
                     /locus_tag="VFB80_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZN34334.1"
                     /translation="MPPISQAWTVATYVLRQRLRGVQRYPLVLMLEPLFRCNLACAGC
                     GKIQFPAHVLRRHLTPEQCWAAADECGAPIVSIPGGEPLLHPQIGEIAAGLVARRKFI
                     YLCTNALKLEESLPQFRPSKYLAFSVHLDGPREEHDRAVCREGTYDIAVQGIRAARRR
                     GFRVTTNTTLFRGADPARIRGFFDELMKLGVEGMMISPGYSYEKAPDQEHFLARQQTV
                     DLFRRILERAKRSWRFNQTPLFLEFLRGRYDLECLPWGSPSYSIFGWQRPCYLLGEGY
                     CPTFRELMETTDWDRYGRQSGNPKCRDCMVHCGYEPSAVAATFGTFRGIAATARTTLL
                     GIS"
     gene            complement(5767..7827)
                     /gene="shc"
                     /locus_tag="VFB80_10970"
     CDS             complement(5767..7827)
                     /gene="shc"
                     /locus_tag="VFB80_10970"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZN34335.1"
                     /translation="MDLGQPEPIPRPHFEGEPLGEDRELPRRAGAAAALARDWLLAQQ
                     RPDGHWCGELEGDTILESEYLLLLAWLGREQSPLAKKCAAYLVEKQLPTGGWAMYPGG
                     KLEISGSVKAYFALKLTGHDAHADYMRLARAAIREAGGADAINSFTRFYLALLGQISY
                     DQCPAVPPELVLLPAWSPINLYRMSAWSRTIVVPLSIMWAKRPRRELPPERGIRELFL
                     SDPAEWPQLRCPGLPRERGWFSWERFFRRSDRVLKWLEARRVRPLRRRALAAAERWMT
                     TRFAHSDGLGAIFPPIIWSIVALKCLGYSDDSAELRYNFDQLAALTIEQQHTARLQPC
                     LSPVWDTAIALRALAASGIAAEEPAAALATDWLLDKEVSRRGDWATHADAPPAGWFFE
                     YHNEFYPDVDDTAMVMIALAELEKFKVQSSTFKVGEVDRASAARRRGLVGACGRAQQW
                     LLAMQNRDGGWGAFDKDNDAEFLCRVPFADHNAMIDPSTPDLTGRAIEALTMWGTRAA
                     EPAVERGVAFLRRTQERDGAWGGRWGVNYIYGTWQALVGLAAAGVPAEDPAVKKGANW
                     LLAHQHACGGWGESADSYEQPELRGKGPVTASQTAWAVLGLLAAGLARHKAVERGIYY
                     LLDTQREAGDWDELEFTGTGFPRVFYLRYHYYPVYFPLLALAEYQRRTHAECGMRNAE
                     WQQR"
BASE COUNT         1491 a         2627 c         2514 g         1353 t
ORIGIN      
        1 caggagcgcg aggtcagcat cggccggcag acgttcgagc tgcccgatcc gttcttcacc
       61 atcgccacgc aaaacccgat cgagcaggaa ggcacttatc cgctgcccga ggcccagctc
      121 gaccgcttca tgttcaacat caaggtcggc tatcccaccg ccgacgagga ggagaaaatc
      181 ctcacgcaga cgacccggac ggagaaaatc gaggtccgca agattctttc ctccgtggcg
      241 atcgtgcggc tgcaaaagct ggtgcactcg gtcgcggtca gcgagtacat cgtgaagtac
      301 gtctcgcggc tggtccgcgc cactcgcccc aaggacgaga cggccccgca gtttgtcaag
      361 gacctcgtcg attggggcgc gggcccccgc gccggccagt tcctgatcaa cggcggcaag
      421 gccctggccg ccatggacgg ccgcttcagc gtcgccatcg acgacgtcaa gcggatcgcc
      481 atccccgtcc tccggcaccg cctcagcacc aacttccagg ctcaggcaga aggcatgtcc
      541 accgaagacg tcatccagcg actgctgaaa gaggtgaagg agccggaagt ggcgaagttt
      601 gcgtgagcga gtgctggtgc tgggggctag tgctagggac attcatcgtc gggcccgagt
      661 tttcgtcgta aggccttttg cagacttcgt agcatctttc caatttgatc agccttagtt
      721 tccagactcg acaccgactt ctgtgtcgcg tacccgagct tcacgcagac cacaaagtag
      781 gtttccactt cggccaatga accaagcgaa tacgagaggt gatgaacata ctctttcgtc
      841 gaatgacggc cgctgccctc ggcgatattg gccgcaatgg atacggcggc acgttgcagt
      901 tgactggaaa gtccatacgt ctcttgcttg ggaaaggtcg ctgacaagcg atagacttcc
      961 tcgataagtt cgatcgactt ctgccacaca atgaggtctt tgaagctgct gatagccatg
     1021 cgaatccccc tagagtttcc tgcaatttgc aagcctagcc tacagttttg atccgtatcc
     1081 tttccggccc cagcactagc ccccagcacc agcactgcaa tggcttccgt cgaaaaatat
     1141 ctcaagccgg aagtcatcaa ccagatcaag cggctggatc tgcgcgcgca gtttgttgtg
     1201 aaagggtttt tgcagggctt gcatgcgagt ccgtttcacg gtttctcggt cgaattcagc
     1261 gagcatcgcc gctacacgca cggagacgat cccaaggaca tcgactggct cgtgttcgcc
     1321 aagaccgaca agtactacgt gaagaagttc gaggcggaga cgaacatcac ggggtacctg
     1381 gtgatggatc tcagccaatc gatgggcttc acgcaccgcc aggagctgac caagttcgac
     1441 tacgcgatct gcctggcggc ctcgctgtgc tacctgatga tctatcagca ggacccggtc
     1501 ggcctggtca cgttcgacca gaagctccgc gacgtaattc cgcccaagag caagcggacg
     1561 cagctgggca acatcctggc gcacctggcg aacctcaagc cggccgggca aaccgacatc
     1621 gccaagagca tcacgcagct cgccgcgatg ctgcgacaca agagcctgat catgctcttc
     1681 tcggacctgc tgcaggatcc cggtccggtg tttcaggccc tccgccgcct gcggcaccgc
     1741 gggcacgacg tgatcttgtt ccatatcctc gacgaagccg aggtccattt tccctatcag
     1801 ggcatgatcg aattcgagga ccccgagacg caggagaaag tcgtcgtcga tgccagcagc
     1861 taccgccgcg actatcaaac cgagatcgag gccttccgcg gcctgtttcg ccaggaatgc
     1921 tttcaaaccg gcgtcgatta cgtcgcgctc gacacgagcc tccagttcga caaggccctc
     1981 ctggaatatc tcagcagccg gcgggcccgc ggctagggcg cgccgcggcg gtagcatcgc
     2041 ccagcgtcat tcattaggct gagcccaaac atattcttgg agcctttcca tgcctcgcct
     2101 ttcgctcgcc gcgctgtgtc ttgcctcgct gccgctcgtt gccactgcag ccgacaagcc
     2161 gatgaacttc gtcgtcgttc tggccgacga catcggggcg aaggagctgg cctgttacgg
     2221 caacacgcag catcagaccc cgcggctgga ccgcctggcc aaggaaggca tgcggtttga
     2281 aacctgctgg gtcacgccgc tctgcacgcc gacccgcgtc ctcacgatga ccggccagta
     2341 cgcttttcgc acgggctact tccagatgtt tggcgccgcg ctctccccgc ggcccaattc
     2401 gcccgaatac cagatcggcg acaaattcac gttcgccgac atgctcaagg cgaagggcta
     2461 cgccaccggc atgtgcggca agtggcagct ctccggcgag catccgacgc tgatccgcga
     2521 ttgcggcttc gatgaatacc ggatgtgggc ctacgagcac aacctgccgg ccggcgtaac
     2581 tcacacgggg cgctacgaag gcggcaatcg cacggcccgc tactggcacc cctcgatcgt
     2641 cgagaacggc aagtacctga ccacgcagcc caatgactac ggcccggatt tgttcaacgc
     2701 gttcgccatc gacttcatca cgcggcacaa ggcggggccg ttttgctttt attacacgat
     2761 gccgctgact cacggcccgc acgaagagac gcccgatccg gctcatcccg gccagcggcg
     2821 gccggcgggc tacaagtcga acgtcgagta ccttgactac ctgatgggaa atctgctcga
     2881 cgcgattgac aagctcggca tcgccgaaaa cacgctgttc atcttcatcg gcgacaacgg
     2941 aacggccggc agcggcaagg gcacggtgac ggagctgggt gtccgcgtgc cgctgatcgt
     3001 gcgctgcccg ggcacagtga aggcgggtgt cgtcagccgc gaactggcca gcggggccga
     3061 cgtgttcgcc acgctgcagg agttttccgg cgcggcgctt ccggccggcc atgtcatcga
     3121 cggcaagagc ttgggccaca cgctccgcgg cgagggtggc cagcaccgcg aatggctctt
     3181 cagctacctc ggccctggcc gcatcctccg cgacgaacga ttcatcctgg aagttcccgg
     3241 ccaggggcag ccgcagaagc tgatcgattg cggccagtcg cgcaatggcg agggctaccg
     3301 tgaggtcacg cagcccacgc ccgaatcgac tgctgcgctg gcccggtttg cgaaactgct
     3361 cgccgatctg cccggcccgg aaaaccaccc cggcctgcgg ctgccggcgg ccggcgaaga
     3421 gggcaaggcc aaaaccaaag caaaagccaa gaagaaaaag gcttaaggca ctcttcaggt
     3481 ttcgtcgtca tacaccaccg cagccggcgg gcgcgtgcgg cggcggaggc cgaagcggcg
     3541 gcggcggacg agcggcccct ggtcttcctc gaagtaatag ccgccgagca gcttgtagaa
     3601 gaacagcagc acgaacgcgc cggccgtcgc cacgcagaag cccagcgggc tgatcggcga
     3661 gatgacgtgc ccccgccaga aatacgaaag gacgccgcag ccgatgatcg tccctgcgac
     3721 gcccatggcg agcgtcgcaa ttgcaccgcc ggggtccttg ccgggcatga tggccttggc
     3781 cagcagcccg acgatcgtgc cgaagccgac ccaaatcagg agctggttgg cccacgcctg
     3841 aacggtctgc atgaagtcgg gattatccat aacggctccg gcctttggcc caagggtgcg
     3901 caaacagcgc gcctgctgtt ggctatcggc cggatgcggt gcgaacttgt ggcggatgta
     3961 acgattgcaa ccgtcccgtg cgacgctagc ggagcccttg ctccgactcc gcggcgaagg
     4021 ttgcctggcg tccggaatgg accaacctag tccattccgc tagcgggcca taagaaaact
     4081 tacgctggtg cgcggcgttg gaactcacga ccgggacgga acttgcgccg cgggcaatcg
     4141 gcgcaagttg gaatcgtgtg gacaaaatgt gtacaagttg cttgcaatcc gagcgcggat
     4201 gtggataatt atgtccacat gtggatcgag tggaaacagc ggtgatgcca ccgcggatca
     4261 ttggcaattc ggattcaacc ttcgcgcggc ttcgagaggc ggtctcgcgc tcgcgcggag
     4321 agcagtggcg aatggcgaat ggcgtttggc ccggtcctgc gcggcgggga attattcgcc
     4381 acccccattt ggctcctaac tgcagagccg taaggcactt gcggccatcg gcggcgaatt
     4441 gttcgccagg tccccggata tcgagccgga atggcgaata attccgagcc aaaagtgcag
     4501 aaaagcctgc gaattgtgca attcccggcg gaatcattcg ccaccgagcg tggcaacccg
     4561 gcgaagaatg gcgggacgac ttgcgccggc gtccacagtc atgcagggtc gcagcaggct
     4621 aacccacgaa gcagatcaag agcttcgcct tcgtgctcta gtgttctttg agtttcaatc
     4681 ggttgccttc acgaaatccc cagcagcgtc gtccgggccg tcgccgcaat gccgcggaac
     4741 gtgccgaacg tggcggcgac ggcgctgggc tcgtagccgc agtggaccat gcagtcgcgg
     4801 cacttgggat tcccgctctg ccggccatag cggtcccagt cggtcgtttc catcagctcg
     4861 cgaaacgtcg ggcagtagcc ctcgccgagc aggtagcagg gccgctgcca gccgaaaatg
     4921 ctgtacgacg ggctgcccca cggcaggcac tccaggtcgt accgcccgcg gaggaattcg
     4981 agaaagagcg gcgtctggtt gaaccgccac gagcgcttgg cccgctccag aatccgccgg
     5041 aacagatcga ccgtctgctg ccgcgcgagg aagtgctctt ggtcgggcgc tttctcgtag
     5101 ctgtagccgg gcgagatcat catcccctcg acacccagct tcatcagctc gtcgaagaag
     5161 ccgcggattc gcgccggatc cgcgccgcgg aagagcgtcg tgttggtcgt gacgcggaac
     5221 ccgcggcgcc tggccgcgcg gatcccttgc acggcgatgt cgtaggtccc ctcgcggcag
     5281 acggcccggt cgtgctcttc gcgggggccg tccagatgca ccgagaacgc caggtatttc
     5341 gacggccgga actgcggcag cgactcttcc agcttcaggg cgttggtgca gagatagata
     5401 aacttccgcc gggccacgag gcccgcggcg atctcgccga tctgcgggtg caagagcggc
     5461 tcgccgccgg gaatcgaaac gatcggcgcg ccacactcgt cggccgccgc ccagcattgc
     5521 tcgggcgtca ggtgacgtcg caggacgtgg gcggggaatt gaattttgcc gcagccggcg
     5581 caggccaggt tgcagcgaaa cagcggctcc agcatcagga ccagcgggta gcgctgcacg
     5641 ccgcgcagcc gttgccgcag gacgtaggtc gcgacggtcc aggcctggga gatcgggggc
     5701 atggttcaaa tgcggaatgc ggagttcgga atgcggagtt ctcggtgcgg aataagaaaa
     5761 ctcaacctac cgctgctgcc attccgcgtt ccgcattccg cattccgcat gggttcgccg
     5821 ctggtactcg gccagtgcga gcaagggaaa gtagaccgga taatagtggt atcgcaaata
     5881 gaagacgcgg ggaaacccgg tgccggtgaa ctccagctcg tcccagtcgc cggcctcgcg
     5941 ctgcgtgtcg agcaggtaat agatgccgcg ctccacggcc ttgtgtcgcg cgagacccgc
     6001 ggccaggagg cccagcacgg cccaggcagt ctgcgaggcc gtcacagggc ctttgccgcg
     6061 cagctcgggc tgctcatagc tgtcggccga ttcgccccac ccgccgcaag cgtgctggtg
     6121 ggccaagagc cagttcgcgc ccttcttcac cgcggggtcc tctgctggca cgccggcggc
     6181 ggccagaccg accagcgcct gccaggtgcc gtagatatag ttgactcccc agcggccgcc
     6241 ccaggcgccg tcgcgctcct gggtccgccg caaaaatgcc acgccgcgtt cgaccgcggg
     6301 ctcggcggcc ctcgtgcccc acatcgtgag cgcctcgatg gcccgccccg tcaggtcggg
     6361 cgtgctggga tcgatcatcg cgttgtgatc ggcaaacggc acgcggcaga gaaactcggc
     6421 gtcgttgtcc ttgtcgaacg cgccccagcc gccgtcgcgg ttctgcatgg ccaggagcca
     6481 ttgctgcgcc cggccgcaag cgccgaccag tcctcgccgc cgcgccgcgc tcgcccgatc
     6541 gacttcaccg actttgaaag ttgaactttg gaccttgaac ttttcgagtt cggccagggc
     6601 gatcatcacc atcgccgtgt catcgacgtc gggataaaac tcgttgtgat attcgaagaa
     6661 ccagcccgcg ggcggggcat cggcatgcgt ggcccagtcg ccgcgccgcg agacctcctt
     6721 gtccagcagc cagtcggtcg ccagggccgc cgccggctct tcggccgcga tgccgctcgc
     6781 cgcgagggcc cgcagcgcga tcgcggtatc ccacacgggc gacaggcagg gctgcaagcg
     6841 ggccgtatgc tgctgctcga tcgtcagcgc cgccagctgg tcgaagttat atcgcagctc
     6901 ggcgctgtcg tcgctgtagc ccaggcactt gagggccaca atgctccaga tgatcggcgg
     6961 aaaaatcgcg ccgaggccgt cgctgtgagc gaaccgcgtt gtcatccacc gctcggcggc
     7021 ggccagagct cgccggcgga gcgggcgcac gcggcgagcc tccagccact tcagcacgcg
     7081 atccgaccgg cggaagaacc gctcccacga gaaccagccc cgctcgcgcg gcaggcccgg
     7141 gcagcgcagc tgcggccact ccgccggatc gctgagaaaa agctcgcgga tgccgcgctc
     7201 cggcggcagc tcgcgccgcg gccgcttggc ccacatgatc gacagcggca cgacgatcgt
     7261 ccgcgaccag gccgacatcc ggtacaggtt gatcggcgac cacgccggca gcaggaccag
     7321 ctccggcggg accgccgggc attggtcgta ggaaatctgc cccagcaagg ccaggtagaa
     7381 ccgcgtaaag ctgttgattg cgtccgcgcc gccggcttcg cgaatcgcag cccgcgccag
     7441 ccgcatgtag tcggcgtggg cgtcgtggcc ggtgagcttg agagcgaaat aggccttgac
     7501 gctgccgctg atctccagct tgccgccggg atacatcgcc cagccgcccg tcggcagctg
     7561 cttctcgacg agataggcgg cgcacttttt cgccagcggc gactgctcgc ggccgagcca
     7621 cgccaggagc agcaggtatt cgctttcgag gatcgtgtcg ccttccagct cgccgcacca
     7681 atgcccgtcc gggcgctgct gagccagcag ccagtcccgc gccagcgccg cggccgcgcc
     7741 ggcgcggcgc ggcaactcgc ggtcttcgcc gagcggctcg ccctcgaagt gcggtcgcgg
     7801 aatcggttcg ggctgtccca gatccatgac gcttgtgggg tgcgaggggg gctccgcggc
     7861 cgcccgccgc aaagcctgca tttgcctata cgctaagggc ccgccgaatg gcaagagaga
     7921 atccgtctcc cgcgcaagtg tcccgcaaac attccgagcg aaatcggttg acctgccgcg
     7981 gatcg
//
LOCUS       DASRJB010000415        13814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3781392, whole genome shotgun sequence.
ACCESSION   DASRJB010000415 DASRJB010000000
VERSION     DASRJB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781392"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="hemB"
                     /locus_tag="VFB80_10975"
     CDS             <1..786
                     /gene="hemB"
                     /locus_tag="VFB80_10975"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZN34336.1"
                     /translation="KLGLGGIILFGIPAEKDAAGRDSYSASGIVQQAIRAAKNTAPEL
                     LVITDVCFCEYTDHGHCGLIDDRTGRMDVNNDATLELLGRQAVSHAQAGADMVAPSGM
                     MDGMVRAIRTALDGASFPHVPIMSYAAKYASAFYGPFREAAESTPKFGDRRAYQMDPA
                     AGSGQALREVELDLAEGADIVMVKPALAYLDIIRQTAERFPGVPLAAYNVSGEFAMVK
                     AAAERGWIDERAIALESLTAITRAGAGIILTYWAKDVAAWLSS"
     gene            814..1575
                     /locus_tag="VFB80_10980"
     CDS             814..1575
                     /locus_tag="VFB80_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34337.1"
                     /translation="MADGAGATTFSSDPAAESPVRTAGGAVHLLTIRVVGAARPEQLA
                     DLRGWREEQLQLTPKDAKLGRRFKTRIQQRFFTEYDRLLDQNREVETLLNRRLASLIR
                     RELYRRQSDYRLLAYTILPNHVHVVLESRAAADSLAAAHSGQAQQIPERMDEQPDVCS
                     PLVDYLRGLKVATTEAAAALEGGGLPLPWHDESFDYWVRSEAELDAIVDYLAHNPVSA
                     GLARAAEQWFFCSAHDRFLHDGESCGWLPGLPAPK"
     gene            1602..2324
                     /locus_tag="VFB80_10985"
     CDS             1602..2324
                     /locus_tag="VFB80_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN34338.1"
                     /translation="MKSVQLRRELVESFEVYPYSIPAIKHLTALELHPCVTFFVGENG
                     TGKSTLLEAIAVAEGFNPEGGSRHAMFATRPTHDESLPRKLQLGKSKIPRGDSFFLRA
                     ESFYNLSSYVDQLGFPPDKNPYGPVPLHQQSHGEAFLTLMLQRFTGNGLYLFDEPEAA
                     LSPQRQLTFLAGLHELVKRGGQLIIATHSPIIMAYPDSLILHFTASGIAPIAYEETEH
                     FKVTRAFLNRRELMLKELMEGD"
     gene            2827..4074
                     /locus_tag="VFB80_10990"
     CDS             2827..4074
                     /locus_tag="VFB80_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34339.1"
                     /translation="MRSRPAWIVSLALWIVAPTAPAADWLAWRGPLGTGYCEEKSLPL
                     TWSQTENVKWSVPLDGDGNSSPIVVGPKVFITHAPKPSNLRGLRCYDRDTGKLLWKHE
                     VAYTEKEETHGTNPPCASSPTSDGQRVVAWYGSAGLFCYDLEGKILWQKDLGKVEHIW
                     GYGSSPLLHENLVILNFGPGLNAFVAAFDKQSGDEVWRKEFPEQRSPKVEDYRGSWST
                     PVVATVGGKSLLLLSLPDRLFACDPKSGQEVWSCAGLANKLIYTSPLVAGDIVVVMCG
                     YGGPAIAVKGGGQGDVTETHKLWTHPRNSQRVGSGVVVDGLVYILNENGSFWCMDPQT
                     GERKWEQPRGGGTSWSSMTYAAGRIYISNTPGTTFVLEANPKEFKLLAENQLNETIRA
                     TPAFSNGQIFIRTYKNLYCIEAK"
     gene            4071..6587
                     /locus_tag="VFB80_10995"
     CDS             4071..6587
                     /locus_tag="VFB80_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HZN34340.1"
                     /translation="MNELARPELLAPAGDRDCIRAAIENGADAVYFGLDAGFNARARA
                     KNFSPSELPEILAELHRRGLAGYVTLNTLVFTSELAEVESLVRTVAAAGVDAVLVQDL
                     GLVRLIRAVCPDLPIHASTQMTMTSSQTIAAAAELGASRVVLARECSLEEIRRIRRET
                     ALPIEVFVHGALCVAYSGQCLTSESLGGRSANRGQCAQACRLPYDLICDGKNVDLGEQ
                     KYLLSPQDLAAFDLIPQLIDAGVCSFKIEGRLKTPEYVANIVKHYRRAIDEALAGRAA
                     RFTPRDVEEMELSFSRGFSPGWLAGCDHKLLVPAISSAKRGVLLGHVRGVRGGRVIIE
                     LADRAVKRGDGVSFDCGKAVDDEQGGRVFEVFQGRRSLAEPISSGVVELSFAHGAIDF
                     SQIQPGMRVWKNDDPELTSRLRKSFTAADPQRRTAVDLSVRAAALQPLSIAAITETGH
                     SVQVAARQPCEEARKHPLTEQTLREQLGRLGGTPFVLRGLAAQIDGSPMVPFSVLGEL
                     RHELVRRLEEVSQAPPTRRMATQSMLPELRPAVVGAASQSHPDAQGQLHVLCRSLPQL
                     REVLEFGVKSIYADFADIREYREAVGAGREQGAQVWLATPRIQKPDEMGIFHALARHK
                     SAGILVRNLAGLRFFREQGVPAIADFSLNAANELTAQYLHEQGAARIAPSYDLNREQL
                     LDLVGAVPPQWLEVVIHQHMPMFHMEHCVFCAVLSPGTNKHNCGRPCDVHEVKLRDRL
                     GMEHRLTADVGCRNTLFNAVPQSGAEIVPQLWAAGVRHFRVELLDDSGSPLVELIDLY
                     RQLLSGGVTGREVWTRLKASNRVGVTRGTLEERRNPLAIL"
     gene            complement(6664..8256)
                     /locus_tag="VFB80_11000"
     CDS             complement(6664..8256)
                     /locus_tag="VFB80_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN34341.1"
                     /translation="MSASTTAPAPTNVRYVVVGLCAAMSVLLYLDRFAISPATETMLR
                     ELSLTKEQFGQAIGAFFLAYALMQIPSGWLTDTLGGRWTLAAYVVAWSLATIGLGFAK
                     GLAGIWVLRLILGIAQAGAYPAAAALLKRWIPLSGRGLANSSVAMGGRCGLLVALMIT
                     EPLMLLVGRLLNWDAGTWRVVLGMYGVLGLLWAAAFVRLFRNSPSEHPWCNAAEVKLI
                     ADDAPQQGQETAAASGIGAVCGLLFAVNIGWIVLIGNLPAVSAERVKVLAGLLHNELA
                     ASLVVRAVPEFLGLIGTLALCVLTTWLLTAWFGQAGERFRLPVGEMLASKEVWIMCVN
                     QFCVNIGWVFLATWLPQYLIENHGAYLKAQIGDEKIVAPLITAVVQLAAIAGGMSGGK
                     ATDVFVRRFGRAWGRRLPGMISGVAVCGLYLLVPRLAGLWWFVGVMILVAFTIDFGLG
                     ASWASYQDIGGRHVASVLGVGNMCGNLGAAIFARLIGYLADRDQWQVVFFISAGAMAM
                     VSLCWLLFDASRPVVREGKPAL"
     gene            8429..9694
                     /locus_tag="VFB80_11005"
     CDS             8429..9694
                     /locus_tag="VFB80_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34342.1"
                     /translation="MRPGGSQFAIAVALWCGCAPAAAQTIQFQKGAADAAPRLGPSTV
                     SPPTITVPGGSNVITAPPAGAATPINPYTGTTAAPFDPYSTSGSSAYQFWSNTPPSNG
                     NGVFAQPPASPYAATTPLAQPPGSFPTYGPPAAPPGGVASPQYVFPNGVGPQWDLSQS
                     IRFIQDLRLRHTYVGGGDDPTDLGINDTETAVTFTIPNFLTTGQPLFISPAFALHLWD
                     GPSFMAADLPPTAYSAYLDAQWASDPNLQIGAEVGFRVGVYTDFNTLNDDSLRIQGLG
                     LGVLRLTPTLTAKIGVMYLDRNKIKILPAGGLLWTPTPQVRFDFFFPQPKLSAYLTTV
                     GRFELWGYVAGEYGGGAWTIERADGTSDAVDINDIRISSGIEWTGPRGLTGFVEAGFV
                     FRRQVIYVVTPADSFNPDDSYMIRAGLSF"
     gene            9766..10809
                     /locus_tag="VFB80_11010"
     CDS             9766..10809
                     /locus_tag="VFB80_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34343.1"
                     /translation="MALLALVAAARAQGDPRLLPAPAVPVDRLPAPGAAPPGPPAGID
                     GPLTTLEALPAPGAGPWDEQPLPESPVEELLTDEDLYYFPPQKISSHKNGFFQLFSLS
                     ATWFGNANDPDDLGGTEIDTFLTVALPAPIVEWPLLITPGFNMTLIDGPTVTDLPTRL
                     YLTYVDFMWLPVVVNRWKALLSVAPSVFGDFDTGEFRLTGKALAIFDWIPGRVQLIGG
                     VLYLNRDNVRILPAGGVIWTPADWTRFELLFPKPKLAVRTGVGPGFEDWIFGTAEFGG
                     NTWPIVRDSGLKDNVTYIDYRLIAGFERKLNGGAGYRLEGGYVFGRSITFTSGNGDFD
                     PQNTFLIRGVITY"
     gene            complement(10933..13341)
                     /locus_tag="VFB80_11015"
     CDS             complement(10933..13341)
                     /locus_tag="VFB80_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34344.1"
                     /translation="MKMPKMKFDTAKLQLFLFHHVEKLVLALVVCVMGLLIWQGFKLE
                     GLDKSKTPQGLLQKSDELQAFIDTANRWTEVVGPEREAQVPLNIAQQVEVGQKSTDAL
                     AYTLPVSINRPSFPKHNPRLDPELLPPEHVIAYGWIGPLAVFAKQNDEDALYERPAAD
                     LEPPVVQKPKKAKPKKGTGPGGEAAYGSPDGGYGPGMPGPGGAKKKKGRNSRYDPTDA
                     TLGSGDYMPQPGGPGGYGYGNGMSPETIYPESVSEGFVLQMAEATIARPTYAMTVMAV
                     VPILKQSERFEKALADSLDFDSSRDFPWYLKFEVQRADVTADPSGAIDWTKAVLIDPM
                     KVQIQQHGRPADMNRPEVKPDWAGAVPEVVDINYVDPKLTHPAPPVLQRDLWSLLTHP
                     DVPLATAMQMGGEYGPGAPGAAPPADESGDLFANPNGAGGAGMPGMPGMPGMPGPGGR
                     GSPDGGYGGGYGRMGGEAGMGMGRMGGMSPYGGSGYGGGYGRMGGYGGYGGDSGGYGQ
                     AGPSLTPPKYKLIRMTDTQVQPRRKYVYRVRVWLHDPNHPATSMGFIAPSIASLDDKV
                     KKRIKEVDDEVKAGKRKAFEASIIMTEWSAPSAPTELPPPDRFFAVSMPEVRKRELVK
                     GKPEVADDQPKAEALIVKFDHRKVVDIGSDQTVARGAVLNLVPDPKADPALDPKVVVL
                     KVIHPVTKQFVELPKYGFATGAIVADIQGGEMIPPTDKANPNSLQAPCEMLVVDATGR
                     LRVLNETDDIEGIRRFGIPKPPPMPTGQPGSEYGPGEAGYDPLGSPDGGTGGRPRRPT
                     RGGS"
     gene            complement(13418..>13814)
                     /locus_tag="VFB80_11020"
     CDS             complement(13418..>13814)
                     /locus_tag="VFB80_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34345.1"
                     /translation="GPGPGGFPGGSPDGGRGSMMPSPGGFSGGFGGGTAGGGYGGATG
                     GFGGGFGGGYGGGDGGFGGGARGSVTPGDPLRDAMVDQNLIEVELYGIVYIYNPVNKA
                     QLGLEAPPGATVAAPPPIPVTPTSTTATP"
BASE COUNT         2355 a         4601 c         4504 g         2354 t
ORIGIN      
        1 aaactcggcc tgggcggcat tattctgttc ggcattccgg ccgaaaagga cgccgcgggc
       61 cgcgactcgt acagcgccag cggcatcgtg cagcaggcga ttcgcgccgc gaagaacacc
      121 gcgccggagc tcttggtcat caccgacgtc tgcttctgcg aatataccga ccacggccac
      181 tgcggcctga tcgacgaccg caccggccgg atggacgtga acaacgacgc cacgctggag
      241 ctgctcggca ggcaggccgt cagccacgcc caggccggcg cggacatggt cgcgcccagc
      301 ggcatgatgg acgggatggt gcgggccatt cgcacggcgc tcgacggcgc gagcttcccg
      361 cacgtgccga tcatgagcta cgcggccaaa tacgccagcg cgttctatgg cccgttccgc
      421 gaggcggccg aaagtacgcc caagttcggc gaccgccggg cctatcagat ggatccggcg
      481 gccggcagcg gccaggcgct gcgcgaggtc gagctcgacc tggccgaagg ggccgacatc
      541 gtgatggtca agccggccct ggcgtacctc gatatcatcc ggcagacggc cgagcggttt
      601 cccggcgtgc cgctggcggc gtataacgtg tcgggcgaat tcgcgatggt caaggccgcg
      661 gccgaacggg gctggatcga cgagcgggcg atcgcgctcg aatcgctgac ggcgatcacc
      721 cgcgcggggg caggaatcat cctgacctac tgggccaagg atgtggccgc gtggctttcg
      781 agctagaggg atcgcggcag cggggaaact gcgatggccg acggcgcggg cgcaacgacg
      841 ttttcatccg acccggccgc cgagtcgccg gtgcggaccg cgggcggcgc ggtgcacctg
      901 ctgacgatcc gcgtcgtggg cgcggcgcgg cccgagcagc tggccgacct ccgcggctgg
      961 cgcgaagagc agctccagct gacgcctaag gacgccaagc tcggccgccg cttcaaaacc
     1021 cgcatccagc agcggttctt tacggaatac gaccggctac tcgaccagaa ccgggaggtg
     1081 gaaacgctcc tcaaccgccg gctggcgtcg ctgatccgcc gcgagctgta ccggcggcag
     1141 agcgactacc gcctgctggc ctatacgatt ctgccgaatc acgttcacgt ggtgctcgag
     1201 tcgcgcgcgg cggccgattc gctggccgcg gctcacagcg ggcaggcgca gcaaatcccc
     1261 gagcggatgg acgagcagcc cgatgtttgt agtccgctcg tcgattatct gcggggcctg
     1321 aaggtggcga cgaccgaggc ggcggcggcc ttggaaggcg gcggcttgcc cctgccttgg
     1381 cacgacgagt cgttcgacta ctgggtccgc tccgaggcgg agctcgacgc gatcgtcgat
     1441 tacctggccc acaacccggt ctcggccggc ctcgcccggg cggcggaaca gtggttcttc
     1501 tgttcggccc acgaccgctt cctgcacgac ggcgaatcct gcggctggtt gcccggtttg
     1561 cccgccccga aatgaatcga gcgcccacgg atccgccgtt tttgaaatcc gtccagctgc
     1621 ggcgggagct ggtcgagtcg ttcgaggtct acccctattc gatcccggcg atcaaacatc
     1681 tgacggcgct ggagttacat ccgtgcgtaa cgttctttgt cggcgagaac ggcacgggca
     1741 aatcgacgct cctggaagcc attgccgtcg ccgagggctt caaccccgaa ggcggctcgc
     1801 ggcacgcgat gttcgcgacc cggccgacgc acgacgaatc gctgccgcga aagctgcaac
     1861 tgggcaagag caagattccc cgcggcgaca gcttcttcct gcgggccgag agcttctaca
     1921 acctttccag ctacgtcgat cagctcggct ttccgccgga caagaacccg tacggcccgg
     1981 tgccgctgca ccagcagtcg cacggcgagg cgttcttgac gctgatgctc cagcggttca
     2041 ccggcaacgg cctgtacctc ttcgacgagc ccgaggccgc gctcagcccg cagcggcagc
     2101 tcacgttcct ggccggtctg cacgagctgg tcaagcgcgg cggccaactc atcatcgcca
     2161 cgcactcgcc gatcatcatg gcctatcccg actcgctgat cctgcacttc acggcttccg
     2221 gcatcgcgcc gattgcgtac gaagaaaccg aacacttcaa ggtcacgcgg gcgtttttga
     2281 accggcgcga gctgatgctc aaagagttga tggagggcga ctagtggaac ctcgtatcgg
     2341 cctcattacg ctcggcgtga tggtcggcct ttcccgccgc ctccttgact ctatacagcg
     2401 gtttactaag atagaaagct tacaaacggc aggaaaggcc ctgctactcc cccaaaatgg
     2461 ttgaatgcga ataatgcgca tagccgccga ctcgcattct tggacgcaaa gcgttatgga
     2521 gcgagtactt gcaacgaaaa aatatccttc agacaaaaaa ctccagtcat gcgcgcaggc
     2581 cgcgtgttct cgcatgcgac gatcacccga tcgccccagc gcataatgcg cataatggct
     2641 gagccttcat ttgtgatgta aacccatgtg aataaagcac ttaccttcag taattcgacc
     2701 gcggagaaat ttgcgggtca tgcgcgcatg atgctcgtgg cgtatccacg tgagaaattg
     2761 gtaggctgaa agccccttcc cttactgcac cggccatcgg gcaatcccac cgctcggagc
     2821 tcttccatga ggtcccgccc tgcttggatc gtgtcacttg ccttgtggat cgtcgcgccg
     2881 accgcacctg ccgccgattg gctagcgtgg cgcgggccgc tgggaacggg ctattgcgag
     2941 gaaaaatcgc tgccgctgac ctggtcgcag accgagaacg tgaagtggag cgtgccgctg
     3001 gatggcgacg gcaattccag cccgatcgtc gtcgggccga aggttttcat cacgcacgcc
     3061 cccaagccga gcaaccttcg cggcctcagg tgctacgacc gcgacaccgg caagctgctc
     3121 tggaagcacg aggtcgccta caccgagaaa gaggagacgc acggcaccaa tcccccctgt
     3181 gcctcgtcgc ccaccagcga cggccagcgg gtcgtcgcct ggtacggctc ggcaggcctc
     3241 ttttgctacg acctggaagg caaaatcctc tggcaaaagg acctgggcaa ggtcgagcac
     3301 atctggggct acggctccag cccgctgctg cacgagaacc tggtgatcct caacttcggg
     3361 ccgggcctga acgcctttgt cgcggcgttc gacaagcaaa gcggcgacga agtctggcgg
     3421 aaggagtttc ccgagcagag gtcgcccaag gtcgaggact accgcggctc ctggagcacg
     3481 cctgtcgtcg cgactgtcgg cggcaagtcg ctgctcctgc tcagcctgcc cgaccggctg
     3541 tttgcctgcg atccgaaatc aggccaggaa gtctggtcgt gcgccggcct ggccaacaag
     3601 ctcatctata cctcgccgct ggtggccggc gacatcgtcg tcgtcatgtg cggctacggc
     3661 gggccggcga tcgcggtcaa gggcggcggc cagggcgacg tgaccgagac gcacaagctc
     3721 tggacgcatc cgcgcaactc gcagcgcgtc ggctcgggcg tcgtcgtcga tggcctggtg
     3781 tatatcctca acgagaacgg ctcgttctgg tgcatggacc cccagaccgg cgagcggaag
     3841 tgggagcagc cgcgcggcgg cgggacgagc tggagctcga tgacctatgc cgcggggcga
     3901 atttacatca gcaacacgcc gggaacgacg ttcgtcctgg aggcgaatcc gaaagagttc
     3961 aagctgctcg ccgagaacca gctcaacgag acgattcgcg ccacgcccgc gttttccaac
     4021 gggcagattt tcatccgcac gtacaaaaac ctgtactgca tcgaggcgaa gtgaacgaac
     4081 tggcccgtcc cgaactgctg gccccagcgg gcgaccgcga ctgcatccgc gcggcgatcg
     4141 agaacggcgc ggatgcggtc tatttcggcc tggatgccgg cttcaacgcg cgagcgcggg
     4201 ccaagaattt cagccccagc gagctgccgg agatcctggc cgaactgcac cgccgcggcc
     4261 tggcgggcta cgtcacgctc aacacgctcg tcttcacgag cgagctggcc gaggtcgaga
     4321 gcctcgtgcg cacggtcgcc gcggcgggcg tcgatgcggt gctggtgcag gacttgggcc
     4381 tggtgcggct gattcgcgcg gtctgccctg atctgccgat tcacgccagc acgcagatga
     4441 cgatgaccag cagccagacc attgccgcgg cggccgagct gggcgcgagc cgcgtggtgc
     4501 tggcccgcga atgctcgctg gaagagattc gccgcatccg ccgcgagacg gcgctgccga
     4561 tcgaggtgtt cgtccacggc gcactgtgcg ttgcctattc cggccagtgc ctgacgagcg
     4621 aatcgctggg cggccgcagc gccaatcgcg gtcagtgcgc gcaagcctgc cgcctgccct
     4681 acgacctgat ctgcgacggg aagaatgtcg atctagggga gcagaaatac ctcttgagcc
     4741 cgcaggacct ggcggcgttc gatctgatcc cgcagctgat cgacgctggc gtgtgcagct
     4801 tcaagatcga ggggcgcttg aagacgcccg aatacgtcgc gaacatcgtc aagcattacc
     4861 ggcgggcgat cgacgaggcg ctggccggcc gcgcggcccg gttcacgccc cgcgacgtgg
     4921 aagagatgga gttgtcgttc agccgcgggt tctcgccggg gtggctggcg ggctgcgacc
     4981 acaagctgct ggtcccggcg atcagctcgg ccaagcgggg cgtgctgctg ggtcacgtgc
     5041 gcggcgtccg aggcggccgc gtgataatcg agttggccga tcgcgcggtg aagcgcgggg
     5101 acggcgtctc gttcgactgc ggcaaggccg tggacgacga gcagggcggt cgcgtgttcg
     5161 aggtgtttca aggccgccgc tcgctggcgg aaccgatttc cagcggcgtc gtcgagcttt
     5221 cgttcgctca cggggcgatc gatttttccc agattcagcc gggaatgcgc gtctggaaga
     5281 acgacgaccc ggaattgacc agccgcttgc ggaagtcgtt tacagcggcc gatccgcagc
     5341 ggcggacggc ggtcgatttg agcgtcaggg ccgctgctct acagccgctg tcgatcgctg
     5401 cgataaccga aacaggacac agcgttcaag tcgcggcacg ccagccgtgc gaggaagccc
     5461 gcaagcatcc gctgacagag caaacgctcc gcgagcagct gggccggctg ggcgggacgc
     5521 cgtttgtgct gcgggggctg gcggcccaga tcgacggcag cccgatggtt cccttcagcg
     5581 tcctcggcga gctgcggcac gagttagtgc gacggctgga ggaggtttcc caggcgcctc
     5641 ccacacggcg gatggcgacc caatccatgt tgccggaact tcggccggct gtggtgggag
     5701 cagccagcca gagccaccca gatgcccagg gccaattaca cgtgctgtgc cggtcgctgc
     5761 cgcagctgcg cgaggtgctc gagttcggcg tgaagagtat ctatgccgac ttcgccgaca
     5821 tccgcgagta tcgcgaggcg gtcggcgcgg gccgcgagca gggggcgcag gtctggctgg
     5881 cgacgccgcg gatccagaag cccgacgaga tgggaatttt tcacgcgctg gccaggcaca
     5941 agtcagccgg catcctggtt cgcaatctcg ctggcctccg gtttttccgc gagcagggag
     6001 tgcccgccat tgccgatttc tcgctcaacg cggccaacga actaacggcg cagtacctgc
     6061 acgagcaagg ggcggcgcgg atcgcgccat cgtacgacct caatcgcgag cagctgctgg
     6121 acctggtggg cgcggtcccg ccgcagtggc tggaggtggt gatccaccag cacatgccga
     6181 tgttccacat ggagcactgt gtgttttgcg ccgtgctctc gccgggcacg aacaagcaca
     6241 actgcggccg gccctgcgac gtacatgaag tcaagcttcg cgaccgcctg gggatggagc
     6301 accggctgac agccgacgtc ggttgccgca acacgctgtt caacgccgtg ccgcagagcg
     6361 gggcggaaat cgtgccgcag ctctgggctg cgggcgtgag gcattttcgc gtcgaactgc
     6421 tcgacgacag cggctcgccg ctggtcgagc taattgatct gtaccggcaa ttgctgtcag
     6481 gcggcgtcac tggccgcgaa gtctggacgc ggctcaaggc cagcaaccgc gtcggcgtca
     6541 cccgcggcac gctcgaagag cggcgaaatc cgctggcgat tctgtaggga tgcccccgtg
     6601 gccgagtgtt gtcttgtagg gaatgccctc tgtggcattc cggaacacca cagagggcat
     6661 tccctacagc gcgggcttcc cttcgcgcac gaccggccgt gacgcgtcga agagcagcca
     6721 gcagagcgaa accatcgcca tcgccccagc cgagataaaa aagacgacct gccactggtc
     6781 gcggtcggct aaatagccga tcagccgggc gaagatggcc gcgcccaagt tgccacacat
     6841 gttgccgacg cccagcaccg aggccacgtg ccgcccgccg atgtcctggt agctagccca
     6901 cgacgcgccc aggccgaagt cgatggtgaa cgcgaccagg atcatcacgc cgacgaacca
     6961 ccacaggccg gccagccgcg gcaccagcaa atacaacccg cagaccgcga cgcccgaaat
     7021 cattcccggc aggcggcggc cccaggcccg tccaaaacgc cggacgaaga cgtcggtcgc
     7081 cttcccgccg gacatgccgc cggcgatcgc cgccagttgc acgaccgcgg tgatcagcgg
     7141 ggccacgatc ttctcgtcgc cgatctgggc cttcaggtac gcgccgtggt tctcgatcaa
     7201 atactgcggc agccaagtcg ccaggaacac ccagccgatg ttcacgcaga actggttgac
     7261 gcacatgatc cagacttcct tgctggccag catctcgccc accggcagcc ggaaccgctc
     7321 gccggcctgt ccgaaccagg cggtgagcag ccaggtcgtc aagacgcaca gggcgagcgt
     7381 gccgatgagc ccgagaaact cgggaacggc ccgcacgacg agcgatgcag ccagctcgtt
     7441 gtgcaataag cccgccagaa ctttgacgcg ctctgcggag acggccggaa gattgccgat
     7501 caggacgatc cagccgatgt tgacggcaaa cagcaacccg cacactgcgc cgatgccgct
     7561 tgctgctgct gtttcttggc cctgctgcgg ggcgtcatcg gcaatcagtt tgacttcggc
     7621 ggcattgcac cacggatgct cgctgggtga attgcggaag agtctgacga atgccgccgc
     7681 ccacagcaga ccgaggaccc catacattcc cagcacgacg cgccaagttc cggcgtccca
     7741 gttcagcagc ctgccgacga ggagcatcag cggctcggtg atcatcaggg cgacgagcag
     7801 tccgcaccgc ccgcccatgg cgacgctgct gttggccagg ccgcggccgc tcagcggaat
     7861 ccagcgtttg agcagcgctg ccgccgccgg atacgcgccg gcctgggcga tgccgaggat
     7921 cagccgcagc acccagatgc ccgccagacc cttggcgaag cccagtccga tggtggccag
     7981 cgaccaggcg acgacgtagg cggcgagcgt ccagcgtccg cctaaggtgt cggtcagcca
     8041 gccgctggga atctgcatca gggcgtaggc caggaagaac gcgccgatgg cctggccgaa
     8101 ttgctccttg gtgagcgaca gctcgcggag catggtctcg gtcgccgggc tgatggcaaa
     8161 gcggtcgagg tagagcagga ccgacatcgc cgcgcataag ccgaccacaa catagcgaac
     8221 gttggtcggc gcgggagcgg tggtgctcgc agacataagc cgctgtattg tgcccaatag
     8281 cggccaaaac gctagcagaa tccgcggcgc ggcgagcccg ctagctgcgg ccgctttctc
     8341 ttgacgccgc gcggcgggcg tgctacttac cccgcgcgtg taaattcgct caagttttct
     8401 acccaagttg ccgagaggcc gttcggccgt gagacctggc ggcagccaat tcgcgatcgc
     8461 ggtggcgctc tggtgcggct gcgcgccggc tgcggcgcag acgattcagt tccagaaggg
     8521 cgcggccgac gccgcgccgc ggctggggcc gagcaccgtc tcgccgccca cgatcaccgt
     8581 tcccggcggg agcaacgtaa tcactgcccc gcccgcgggc gccgcgacgc ccatcaaccc
     8641 gtacaccggc acgaccgccg cgccgttcga tccgtactcg accagcggct cctcggcgta
     8701 ccagttctgg tcgaatacgc cgccgtcgaa cggcaacgga gtctttgccc agccgccggc
     8761 ctcgccgtat gccgcgacga cgccgctcgc gcagccgccc ggctcgtttc cgacctacgg
     8821 cccgcccgcc gctccgccgg gcggagtcgc cagcccgcag tacgtctttc ccaacggggt
     8881 cgggccgcag tgggacctct cacagtcgat ccgcttcatc caggacctgc ggctgcggca
     8941 cacctatgtc ggcggcggcg acgatccgac cgacctgggg atcaacgaca cggaaaccgc
     9001 cgtcaccttc acgatcccca atttcctgac gacggggcag ccgctgttca tctcgccggc
     9061 gttcgccctg cacctctggg acgggccgag cttcatggcc gccgacctgc cgccgacggc
     9121 gtacagcgcg tatctcgacg cccagtgggc cagcgacccc aacctgcaaa tcggagccga
     9181 ggtcggtttt cgcgtcggcg tctacaccga cttcaacacg ctcaacgacg acagcctgcg
     9241 catccagggc ctggggctag gcgtgctccg cctgacgccg accttgaccg cgaagatcgg
     9301 ggtgatgtac ctggaccgca acaagatcaa aattctgccg gccggcggcc tcttgtggac
     9361 gcccacgccg caggtccggt tcgacttctt ctttccgcag ccgaagctct cggcgtacct
     9421 gaccacggtc ggcaggttcg agctgtgggg ctacgtggcg ggggaatacg gcggcggggc
     9481 ctggacgatc gagcgggccg acggcacttc cgacgccgtc gatatcaacg acattcgcat
     9541 ctccagcggc atcgagtgga cgggaccgcg cgggctgacc gggtttgtcg aggcgggctt
     9601 cgtgttccgg cggcaagtga tttacgtcgt gacgccggcg gactcgttca acccggacga
     9661 ctcgtacatg atccgggccg ggctatcgtt ctaggcagcg cgttctaggc agcgggaaga
     9721 ttcgtggagc gggcgatttc atcttcgagt tactggtgcg ccgcagtggc cctgctggcg
     9781 ctcgtcgcgg cggcccgcgc ccagggtgat ccgcggctgc tgcctgcgcc cgccgtgccc
     9841 gtcgatcggc tgcctgcgcc gggcgctgcg ccgcctggtc ctccggcggg catcgacggg
     9901 ccgctgacga cgttggaagc gctccccgct cccggcgccg gcccctggga cgagcagccg
     9961 ttgccggaaa gcccggtgga ggagctgctg accgacgaag atctctatta cttcccgccg
    10021 cagaagatca gctctcacaa aaacggcttc tttcagctgt tctcgctctc ggccacctgg
    10081 ttcggcaacg ccaacgaccc cgacgacctg ggcggcaccg aaattgacac gtttttgacg
    10141 gtcgccctgc cggccccgat cgtggagtgg ccgctcttga tcacgccggg cttcaacatg
    10201 acgctgatcg acggcccgac cgtgaccgac ctgccgacgc ggctgtattt gacctacgtg
    10261 gacttcatgt ggctgcccgt ggtcgtgaac cgctggaagg cgctgctctc ggtcgcgccc
    10321 agcgtgttcg gcgacttcga cacgggcgaa ttccgcctga cgggcaaggc gctggcgatt
    10381 ttcgactgga ttcccggccg cgtgcagctg atcgggggag tgctgtacct caatcgcgac
    10441 aacgtgcgga tcctgccggc gggcggggtg atttggacgc ccgccgactg gacccgcttc
    10501 gagctgctgt ttcccaagcc aaagctcgcg gtgcgtaccg gcgtgggccc gggattcgag
    10561 gattggatat tcggcacggc cgagttcggc ggcaacacgt ggcctatcgt ccgcgacagc
    10621 ggactgaaag acaacgtcac ctacatcgac taccgcttga tcgcgggctt cgaacgcaag
    10681 ctgaacggcg gcgcgggcta tcggctggaa ggcggctatg tgttcggccg cagcatcacg
    10741 ttcaccagcg gaaacggcga cttcgatccg cagaacacgt ttttgattcg cggcgtgatc
    10801 acctactagg ggtctggcaa gaaatgaacg aggccgccgg cggaaaccgc ggcggccccc
    10861 ctgggaattt ctgtagggaa tgccctctgt ggcattcccg gaacgccacg gagggcgttc
    10921 cctacacgga cctcaggagc cgccacgggt tggacgccgc gggcggccgc cggttccgcc
    10981 gtccggactg cccagcggat cgtagccggc ttcaccggga ccatattcgc tgccgggttg
    11041 accggtgggc atgggcggcg gcttgggaat accgaagcgg cgaatccctt cgatatcgtc
    11101 ggtctcgttc agcacgcgga gtcggccggt ggcgtcaacg accagcatct cgcacggggc
    11161 ttgcagcgag ttcggattgg ccttgtcggt aggcggaatc atctcgcccc cctggatatc
    11221 ggcgacaatc gctcccgtgg caaaaccata tttgggcagc tcgacgaact gcttggtcac
    11281 cgggtgaatt accttgagga cgaccacctt gggatcgaga gcggggtcgg ccttgggatc
    11341 gggaaccagg ttgagcaccg cgccgcgggc gacggtctga tccgagccga tgtccacgac
    11401 ctttctgtgg tcgaacttga cgatcagcgc ctcggccttg ggctggtcgt cggccacctc
    11461 cggcttgccc ttgacgagct cgcgcttcct gacttccggc atgctgacgg caaagaaccg
    11521 gtcgggcggc ggcagttcag tcggggcgct gggagcgctc cattcggtca tgatgatgct
    11581 cgcctcaaag gccttccgct tgcccgcctt gacttcgtcg tccacctcct tgatccgctt
    11641 ctttaccttg tcgtccagcg aggcgatgct gggcgcgatg aagcccatcg acgtcgccgg
    11701 gtggttcgga tcgtgcagcc agacgcggac gcgatagacg tacttgcgcc gcggctgcac
    11761 ctgggtgtcg gtcatgcgga tcagcttgta cttggggggc gtcaggctcg ggccggcctg
    11821 accgtaaccc cccgagtcgc caccatatcc gccatagcca cccatgcggc cgtaaccgcc
    11881 gccgtagccg ctgccgccat aggggctcat tccgcccatg cggcccatgc ccatgcccgc
    11941 ttcgccgccc atacggccat acccgccgcc gtagccgcca tcgggcgagc cgcggccgcc
    12001 tgggccaggc attcccggca ttcccggcat tccgggcata ccggcgcctc cggcaccgtt
    12061 gggattggca aacaggtcgc cggattcgtc agccggcggc gccgctcctg gagcgcctgg
    12121 accgtattcg ccccccatct gcatcgcggt ggccagcggg acgtcggggt gggtcagcag
    12181 cgaccagagg tcgcgctgca atactggcgg tgccggatgt gtaagcttgg ggtcaacata
    12241 gttgatgtca accacttcgg ggaccgcgcc cgcccagtcc ggcttcactt ccggccggtt
    12301 catgtcggcc ggccggccgt gctgctggat ctgaaccttc atcgggtcga tgagaaccgc
    12361 cttggtccag tcgatcgcac cgctcggatc ggcggtgacg tcggcccgtt gcacttcgaa
    12421 tttcaagtac cagggaaagt cccggctgct gtcgaagtcg agcgaatcgg ccagcgcttt
    12481 ttcgaacctt tcggactgct tgagaatggg gacgaccgcc atgaccgtca tcgcataagt
    12541 gggcctggcg atggtcgctt cggccatttg cagcacgaat ccttcgctga ccgactccgg
    12601 atagatcgtc tcgggcgaca tgccgttgcc gtagccgtag cctccggggc cgcctggctg
    12661 gggcatgtaa tctccgctgc cgagcgtggc gtcagtcggg tcgtagcggc tgttgcggcc
    12721 cttcttcttc ttagcgccgc cgggtcccgg catgcccggg ccatagccgc cgtcgggcga
    12781 gccatacgcg gcctccccgc cggggcctgt tccctttttc ggcttggcct ttttgggttt
    12841 ctggaccacg ggcggctcca aatcggccgc tggccgctcg tacagcgcat cctcgtcgtt
    12901 ttgcttggcg aaaaccgcca gcgggccgat ccacccgtag gcgatcacgt gctcaggcgg
    12961 caagagctcg gggtcgagcc gcgggttgtg tttgggaaag ctggggcgat tgatcgagac
    13021 tggcagcgta taggccaagg cgtcggtgga cttctgacct acctcgacct gctgcgcgat
    13081 gttcagcggc acctgagctt cgcgctcggg cccgacgact tcggtccagc ggttggctgt
    13141 gtcgatgaac gcctgcaatt cgtcgctttt ttggagcagg ccctgcggcg tttttgattt
    13201 gtccaaccct tccagcttga atccttgcca gatcaagagg cccatcacac aaacgaccag
    13261 cgccagcacc agcttttcga cgtggtgaaa cagaaatagc tgcagcttgg cggtgtcgaa
    13321 cttcattttc ggcatcttca taactttcgc tcctcgctcg tttcagtcgg tcccctgcca
    13381 ttggctgctg accgtctggc ctgccccaac tactgcgtta cggcgtggcc gtcgtgctgg
    13441 tcggcgtgac cggaatcggc ggcggtgcgg ccacggtcgc tcccggtggg gcctccagcc
    13501 ccaactgcgc tttgttgacg ggattgtaga tgtaaacgat cccgtacagc tcgacttcga
    13561 tcaagttttg atcgaccatc gcgtcgcgaa gcgggtcgcc gggcgtgacc gacccccgcg
    13621 cgccgccgcc gaagcctcca tcgccgccgc catagcctcc gccgaaaccg ccgccaaacc
    13681 cgccagtggc gccaccatac ccgcccccgg cggtcccgcc gccaaacccg ccactgaaac
    13741 cgcccggcga gggcatcatc gacccgcggc ctccgtcggg ggagccgcct ggaaatcctc
    13801 ccgggccggg accg
//
LOCUS       DASRJB010000416         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1808647, whole genome shotgun sequence.
ACCESSION   DASRJB010000416 DASRJB010000000
VERSION     DASRJB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808647"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFB80_11025"
     CDS             <1..736
                     /locus_tag="VFB80_11025"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HZN34346.1"
                     /translation="DSVQFEYDGEKIDVRREKLDGIIFYQPTRREFSAPLCRLVDAGG
                     STWALRDVQLAGGSLNAATVGSVNLELPLAAVSKVDFSIGNIAFLSELEPDSGLGDPA
                     VSLQPAAMSYKFGSMLRARAAPPLGADTFRIAGVKYASGLSLHSPVTLVYRVPAGFRW
                     LRLLAGVDDSIVAPGRFDLVILGDGKELVRKTFDGKKLSDKQARAAAPIDLEVAGVRR
                     ISIVLDPADGQDFGDQLDLVEARFTK"
     gene            883..2607
                     /locus_tag="VFB80_11030"
     CDS             883..2607
                     /locus_tag="VFB80_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN34347.1"
                     /translation="MPPAVLDAEKARIAAIHKAIQPTVAVFANEGKGGGSGVVISPDG
                     YALTNYHVVQPAGSYMKCGLADGRLYEAVVVGIDPTGDVGVIKLLGRDDFPTATMADS
                     NDVKIGDWCFAVGNPFLLATDFQPTVTYGIVSGTHRYQPPAGTLLEYTDCIQTDAAIN
                     PGNSGGPLFNANGDLIGINGRGSFEKRGRVNVGVGYAISINQIKNFLGCLKSGRIVDH
                     ATLGATVAMSDDGSVRVSNILESSDAYRRGLRYDDQIVAFGGRPIDTPNGFKNVLGIF
                     PKGWRVPLTYRRDGKETEVLVRLAGVHGEEELIQLVNRRPPQEPAPDRPRPRPGERPM
                     PGEEPKPPPMPMPRPAPGRPQQPAKAPIPPEIQKLIKARPGYANYYFNELNRDRVWSA
                     FAGKCDYASAGGAWKLAGEFAAGGKIEMTLAGEKSEGKFPQGAAIVEAGKDLDQQLDP
                     PGSGGVLAALHLWRQLLISGPQKFGDVVYYGTAPYAGIAGQAEVLVATHNVAEVNFVF
                     DPASGQLALLEMTADPDADPCEIRFGDYRDVGGRLLPHRLEVRSGDAVFGTIQLQQVE
                     LSPAETKP"
     gene            2604..>2969
                     /locus_tag="VFB80_11035"
     CDS             2604..>2969
                     /locus_tag="VFB80_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN34348.1"
                     /translation="MSKVAGTLRVPSLLVSLLLALAVPRGAAAQASFAEVAAQTQPKI
                     VKIFGAGGLRSLEAYQSGFLISAEGHILTVWSYVLDSDGVTVYLSDGRKLTATVVGMD
                     PRTEIAVLKIDADNLPYFNL"
BASE COUNT          518 a          966 c          988 g          497 t
ORIGIN      
        1 ggacagcgtg caattcgagt acgacggcga gaagatcgac gtccgccgcg agaagctcga
       61 cggcatcatc ttctatcaac cgacgcggcg cgagttctct gctccgcttt gccggctggt
      121 cgatgccggc ggctcgacct gggcgctccg cgacgtgcaa ctggcgggcg gcagcctgaa
      181 tgcggcaacg gtcggcagcg tgaacctcga gctgccgctg gcggcggtga gcaaggtcga
      241 tttctcgatc ggcaacatcg cgtttctctc ggagctcgag ccggattccg gcctgggcga
      301 cccagcggtc agcctgcagc cggcggccat gtcgtacaag ttcggcagca tgctgcgggc
      361 ccgcgccgcg ccgccgctgg gggccgacac ctttcgcatc gcgggcgtga aatatgccag
      421 cggactgtcg ctgcacagcc ccgtgaccct cgtctatcgt gtgcccgccg gctttcgctg
      481 gctgcggctg ttggccggag tcgatgactc gatcgtcgcg cccgggcggt tcgacctggt
      541 gatcctgggc gacggcaaag agctcgtgcg caagacgttc gacggcaaga agctgagcga
      601 caagcaggcc cgggcggccg cgccgatcga cctggaagtc gccggtgtgc ggcggatttc
      661 gatcgtgctc gatccggccg acggacagga tttcggcgat cagctcgacc tggtcgaagc
      721 gaggttcacg aaatgatgcc agcgattgca aacctgcaga gggatagcgg ccgcggattt
      781 tcgcggatga cacggatcga tcaactgcct gatccgtgtc tgtgtttatc cgcggcgaca
      841 tttgctgtgg tttgtttgct ggcctctgca gcggtagccg aagtgccgcc cgcggttctc
      901 gatgcggaga aggctcgcat cgcggcgatc cacaaggcga tccagcccac ggtggcggtc
      961 ttcgccaacg aaggcaaggg cggcggatcg ggcgtcgtga tctcgccgga cggctacgcc
     1021 ctgacgaact atcacgtcgt ccagccggcg ggcagctaca tgaagtgcgg cctggccgac
     1081 ggccggctgt acgaagcggt ggtcgtcggc atcgacccga cgggcgacgt gggcgtgatc
     1141 aagctgctgg gccgggacga ctttcccacg gccaccatgg ccgacagcaa tgacgtgaaa
     1201 atcggtgatt ggtgcttcgc ggtcggcaac ccgtttctcc tggcgaccga ttttcagccg
     1261 accgtgacct acggcatcgt ctcggggacg caccgctatc agccgccggc cggcacgctg
     1321 ctggaataca ccgactgcat ccagaccgac gcggcgatca acccgggcaa ctcgggcggc
     1381 ccgctgttca acgccaatgg cgacctgatc ggcatcaacg gccgcggcag cttcgaaaag
     1441 cgcggccgcg tgaacgtcgg cgtcggttac gcgatctcga tcaaccagat caagaacttc
     1501 ctgggctgcc tgaagagcgg ccgcatcgtc gatcacgcca cgctcggcgc gacggtcgca
     1561 atgagcgatg acggcagcgt gcgggtcagc aacatcctgg aaagctccga cgcctatcgc
     1621 cgcggcctgc gctatgacga ccagatcgtc gccttcggtg gccgcccgat cgacacgccc
     1681 aacggcttca agaatgtgct cggcattttt ccgaaggggt ggcgcgtgcc gctgacctat
     1741 cgccgcgacg gcaaggaaac cgaggtcctg gtccgcctgg cgggcgtcca cggcgaggag
     1801 gagctgattc aactggtcaa tcgccgaccg ccgcaagagc ccgcgcccga tcgcccgcgt
     1861 ccgcgaccgg gcgagcggcc gatgcctggc gaagagccca agccgccgcc gatgcccatg
     1921 ccgcgtcccg cgccgggccg gccgcagcag ccggctaaag cgccgattcc gccggagatt
     1981 cagaagctca tcaaggcccg acctggctac gccaactatt acttcaacga gctcaatcga
     2041 gaccgtgtct ggtccgcgtt tgcgggcaag tgcgactatg cctccgccgg cggcgcgtgg
     2101 aagctggcgg gtgaatttgc cgccggcggc aagatcgaga tgacgctggc tggcgaaaag
     2161 agcgagggca aattcccgca aggagccgcc attgtcgaag ccggtaagga tctcgatcag
     2221 cagctggacc cgccgggcag cggcggagtt ctcgccgcgc tgcacctctg gcgacagttg
     2281 ctgatctccg ggccgcagaa gttcggcgac gtggtctatt atggcacggc gccctatgcg
     2341 ggcatcgccg gccaggcgga ggtgctcgtc gccacccaca acgtcgccga ggtgaacttc
     2401 gtcttcgatc cggcgagtgg gcaattggca ctattggaaa tgacggccga tcccgacgcc
     2461 gatccctgcg aaatccgctt tggcgactat cgcgacgttg gcggccggct gctgccgcat
     2521 cgcctggagg tccgctcggg cgacgcggtc ttcggcacga tccaactcca gcaagtcgag
     2581 ctttcgccag cggagaccaa gccttgagca aagtagcagg cacactccgt gtgccgtcac
     2641 tcttggtgtc gctcctgctg gcgctcgccg tgccgcgcgg cgctgcggcg caggcctcgt
     2701 tcgccgaagt cgcggcccaa acgcagccaa agatcgtcaa gatcttcggg gccggcggac
     2761 tccgcagcct ggaagcctat cagagcggct ttctgatcag cgccgagggg cacatactca
     2821 cggtatggag ctacgtgctc gacagcgacg gcgtgacggt ctatctgagc gacggccgca
     2881 agctcacggc cacggtcgtc ggcatggacc cgcggacgga aattgccgtg ctgaagatcg
     2941 acgccgacaa cttgccttac ttcaatctc
//
LOCUS       DASRJB010000417         7432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2654136, whole genome shotgun sequence.
ACCESSION   DASRJB010000417 DASRJB010000000
VERSION     DASRJB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654136"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /locus_tag="VFB80_11040"
     CDS             complement(<1..2063)
                     /locus_tag="VFB80_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN34349.1"
                     /translation="MLAASTSLAGDSAPSFELDVLPILTARGCNQGACHGKARGQNGF
                     QLSLLGFDPDFDYAALTQQARGRRVFPAAPERSLILQKATATLPHGGGLRLEPGGSDY
                     ELLLKWIAQGTPRRVENEPKLTAVAVNTPEEFLKSGEALQLEVIATYSDSTTRDVTSR
                     TQYQSSEAGIVSVDPAGLVKAGPIPGEATIMCRYMNLIATCHVAIPLPGSVPSELYAA
                     LPRQNFIDEHVWIKLQSLGITPSQPADDAKFLRRVHLDLIGRLPTPEEVRTFLADGDA
                     QKRSRIVDELLQRPEYADHWAAKWADLLRPNPYRVGIKAVMTYDAWIRDCFRQNKPYD
                     QFVRELVTAQGSTWENGATVLFRDRREPPEMTTLVSQLFLGVRLECARCHHHPFEKYS
                     QDDFYSFAAFFARTGFKGTGLSPPISGSEEIVLLKKTGSVEHPLTRQTLAPRPLFGEA
                     PQLAGDDTDPREALAAWMTSDENPFFAKVIVNRVWADLMGRGLVEPVDDLRATNPPTN
                     GPLLEALAAEFRAQKYDLKKLLRTITTSYVYGLSSLPSDRNAADRQNYSRHYRQRLRG
                     EALLDAVTDITGQGESFSAMPAGSRATELWTTRVQSVFLDTFGRPNPNQDPPCERTSD
                     TTVTQTLHLMNAPQLHQRVTADKSRAAELAATSKTPEEIVEELYLLVYSRLPDAAERE
                     IGRGL"
     gene            2145..3836
                     /locus_tag="VFB80_11045"
     CDS             2145..3836
                     /locus_tag="VFB80_11045"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN34350.1"
                     /translation="MSLPALTQPLQPRCRGAGLWPALLLLSLPAAAAPPLKDQPHLRR
                     PIAAAWIEPGKLLAVANQRSGTVSLVDTTVGEVRDEILIGQHLSDLVAIPQRSLWLAT
                     DDRRHELIVLVRDADSLRVTDRLPLSPHPVDIEVAPSGARILVASLWSRKVTVLRSEA
                     AAGLKPAHVKTLATIEVPFNPRQQAYLMDPLRRYALVADAFAGNLAVLDTHEFKVIAC
                     HEIEGHGIELASSTTLRHHVLHSDVPTRPRDIERGLLIHPATTRLDKADLLDSTGLFF
                     PSKVEWDVHLDRKWTAAGPGVQPLSDYVLDRFADSVIVRGTTTRLGPSPEPNAADRGE
                     ALFFDTRLSAGQVSDAELIPPFRVGPHRKGGWISCHSCHTNGHTCGQLADTLGDGTLG
                     TPKRIPTLLGTRLTDMWAWNGEVRELHEQVRKSLDNTMHAPKYSLQDVLDITAFLHTL
                     PPPPPLEPATEEPADKVQLARGAGLFKTLGCAKCHVPPLTYTSPETYDVGLEDEKGLR
                     KFNPPSLRGVSQGYSFFHDSRAKKLEEVFTVHGHQLDGPLEQAELADLLRFLRSL"
     gene            4216..4491
                     /locus_tag="VFB80_11050"
     CDS             4216..4491
                     /locus_tag="VFB80_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34351.1"
                     /translation="MIQEFPALANFWHCPEYRSRGQDLLQAESAGATCLNLLTRDGAV
                     GVEFTPALEPQHYAELFELAHEFESEAALREVVTAAARRWGRQVVFG"
     gene            4565..5194
                     /locus_tag="VFB80_11055"
     CDS             4565..5194
                     /locus_tag="VFB80_11055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZN34352.1"
                     /translation="MTPLARDFYSRDPITVARELLGRILIRQCDEGLASGRIVEAEAY
                     LSSRDPACHAYRGRNRKNATMFGPPGHAYVYMIHARWCLNAVTEAADLGSAVLIRAVE
                     PLAGIELMQERRRRTDVRDLARGPARLCQALAVTKALDGHDLTSGRELWIAADPAQNG
                     EVLEIVRSRRIGVTSAASRLLRFFVAGSRFVSGRAGRSRPLPAGRAAVV"
     gene            5305..6072
                     /gene="surE"
                     /locus_tag="VFB80_11060"
     CDS             5305..6072
                     /gene="surE"
                     /locus_tag="VFB80_11060"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZN34353.1"
                     /translation="MLILLTNDDGIYAPGLQALEAELRKLGDCVVVAPATEQSGVGHS
                     ITFLQPLVAKQVFDGDRLRGWAVEGSPADSVKLGIFEFCPRRPDVVVSGLNGGLNAGI
                     NVLYSGTVAAAIEGAFFGITSIACSLEFNEHANYLRAAHIARGIIEQVLAQKGPEPQL
                     YNLNIPTVACERTAEVRVVPMGVARYGEQFIKRKDPRGRSYYWATNEPPPPAEGHETD
                     LTALTKGFVTLTPLQYNMTKQPVLSEMERWRLSVPPE"
     gene            6179..6712
                     /locus_tag="VFB80_11065"
     CDS             6179..6712
                     /locus_tag="VFB80_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34354.1"
                     /translation="MSAPPAPPPPPPPPGTQQPAEAPAAAPAAPPGPPPLPVEAKVGV
                     FAGGLDDLARPVSQASPSAAPASPPAEANTEVVKAGKGVGLKGRSLDPHEGLYVTPVK
                     AYFAARERIFFEAEFPTNYKLYNASTDDVPKDFEDLKAKVLDPYGLTKKLPVLPQGHK
                     YVWNPQTQELGVERPKR"
     gene            6766..7338
                     /locus_tag="VFB80_11070"
     CDS             6766..7338
                     /locus_tag="VFB80_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34355.1"
                     /translation="MRWQLSVLAWALSASPAVAADWLTAPSYYTHDTSSGERVAQYSP
                     IGPFYYYARPDYLKSGYRQYRSTIDLGNSSDNLHIVETWGAPVRPYDEWRFPYRPYSA
                     PYPGWGPPYGGLGGGTGGYPYSGGGGFGFGGFPFFPGFPGGIPGGFPSVTPPAVNFAQ
                     PWLDGHYPSYDFRDRSQYWRPYVAPTPTGP"
BASE COUNT         1263 a         2391 c         2440 g         1338 t
ORIGIN      
        1 aacagcccgc ggccgatctc gcgctcggcc gcgtcgggta gccgcgaata gacgagcaga
       61 tacaactcct cgacgatctc ctcgggcgtc ttgctggtgg cggccagctc ggcggcccgg
      121 ctcttgtcgg ccgtgacccg ctgatgcaat tgcggggcgt tcatcaggtg cagcgtctgc
      181 gtcacggttg tgtcgctggt ccgttcgcag ggcggatcct ggttcggatt cggccggcca
      241 aacgtgtcga ggaacaccga ctgcacgcgg gtcgtccaca gctcggtcgc ccgcgagccg
      301 gcgggcattg cgctgaacga ttcgccctgg ccggtgatgt cggtcacggc atcgagcagc
      361 gcctcgcccc gcaaccgctg gcggtagtgc cgcgaatagt tctgccggtc cgccgcattg
      421 cgatcgctcg gcagcgagct gaggccgtag acgtacgacg tggtgatcgt gcgaagcagc
      481 ttcttcaagt cgtatttctg ggcgcgaaac tcggccgcca gtgcttccaa gagcggcccg
      541 ttcgtcggcg ggttcgttgc ccgcaagtca tcgaccggct ccaccaggcc gcggcccatc
      601 aggtcggccc agacgcggtt gacgatcacc ttggcgaaga acgggttttc gtcgctcgtc
      661 atccaggcgg ccagcgcttc gcgcggatcg gtgtcgtcgc ctgccagctg cggcgcttcg
      721 ccgaacagcg gccgcggcgc gagcgtttgc ctggtcagtg gatgttcgac cgagccggtc
      781 tttttcaata gcacgatctc ttcgctgccg ctaatcggcg gcgagaggcc ggtccctttg
      841 aagcccgtgc gggcgaagaa cgcggcgaag ctgtagaaat cgtcctggct gtatttctcg
      901 aatggatggt gatggcagcg ggcgcattcc agccgcacgc ccaggaacag ctggctgacg
      961 agcgtcgtca tttccggcgg ctcacgtcgg tcgcgaaaca gcaccgtcgc gccattctcc
     1021 caagtcgagc cctgggccgt caccagctcg cggacgaact ggtcgtaggg cttgttctgc
     1081 cggaaacagt cgcgaatcca ggcgtcgtag gtcatcaccg ctttgatgcc gacgcggtac
     1141 ggatttggcc tgagcaggtc cgcccacttc gccgcccagt ggtcggcata ctcgggccgc
     1201 tgcaagagct catcgacgat gcgcgaccgc ttctgcgcat cgccatcggc caaaaacgtc
     1261 cgcacttcct ccggcgtcgg cagccggcca atcaaatcga ggtgcacgcg ccgcaggaat
     1321 ttcgcatcgt ccgccggctg cgaaggcgtg atgcccagcg actggagctt gatccagacg
     1381 tgctcgtcga tgaagttctg ccgcggcagg gcggcgtaca gctcgctcgg cacgctgccc
     1441 ggcagcggaa tcgccacgtg gcaagtcgcg atcaagttca tgtaccggca catgatcgtc
     1501 gcctcgcccg ggatcggccc ggccttcacg aggcccgccg gatcgacgga gacgatcccc
     1561 gcttcgctcg actggtactg cgtgcggctg gtgacgtcgc gggtcgtgct gtcggaatag
     1621 gtcgcgatca cctcgagctg cagtgcttcg ccggatttca ggaattcctc cggtgtgttg
     1681 accgcaaccg ccgtcagctt cggctcgttc tcaacccgcc gcggcgtgcc ctgcgcaatc
     1741 cacttcagca gcagctcata atcggaaccg cccggctcca gccgcaggcc gccgccgtgc
     1801 ggcagcgtcg ccgtagcctt ttgcaggatc aggctccgct ccggggctgc gggaaacacg
     1861 cgccggccgc gagcctgctg ggtcagggcc gcgtagtcga aatcgggatc gaaccccagc
     1921 agcgagagtt gaaagccgtt ctgcccgcgg gccttgccgt ggcacgctcc ttggttgcag
     1981 ccgcgagcgg ttaagatggg aaggacgtcg agctcaaaac tcggtgccga gtcgcccgct
     2041 agcgacgtgc tcgcagccaa catgtagaga atcgcaactg agtgggcgca gcgcatcggc
     2101 aaaccgcagg cagggcaagt agcggttgat ataaggcgag ccttatgagt ttacctgccc
     2161 tgacccaacc cctgcaaccg cgctgccgtg gggcgggcct ctggccggcc ctgttgctgc
     2221 tcagtcttcc tgccgcagct gccccgccgc tcaaggacca gccccacctc cgccgcccga
     2281 tcgcggccgc ctggattgag cccggcaagc tgctggccgt cgccaaccag cgaagcggga
     2341 ccgtgtcgct cgtggatacc acggtgggcg aggtgcgcga cgaaatcttg attggccagc
     2401 atctgagcga cctggttgcg attccgcagc ggtcgctgtg gctggcgacc gacgacaggc
     2461 gccacgagtt gatcgtgctc gtgcgagatg ccgattcgct gcgcgtgacc gatcgcctgc
     2521 cactcagtcc gcatccggtg gatattgaag tcgcaccaag cggagcgcgg attctcgtcg
     2581 cttccctctg gtcgcgcaag gttaccgttc ttcgttctga ggcggccgcc ggcctgaagc
     2641 cggcgcacgt caaaacgctt gccacgatcg aggttccatt caatccgcgt cagcaggcgt
     2701 atctcatgga tccactccgc cgctacgcgc tggtggcgga tgcatttgcc ggcaatctgg
     2761 cggtgcttga cacgcacgaa ttcaaggtga tcgcatgcca cgaaatcgag ggacatggaa
     2821 tcgagctggc gtcgagcact accctgcggc atcacgtatt gcatagcgac gtgccgactc
     2881 gtccgcgcga catagaacgc ggattgctca ttcatccagc gacgacgagg ctcgacaaag
     2941 ccgacctgct cgattccacg gggttgtttt ttccgtcaaa agtcgaatgg gacgttcatc
     3001 tggatcggaa atggaccgcc gccggccctg gcgttcagcc gctttcagac tatgttttgg
     3061 acagattcgc cgattccgtc atcgtccgtg gaacgacaac gcggctcggt ccgtctcccg
     3121 aaccgaatgc cgccgatcgc ggcgaggcat tgtttttcga tacgcggctg tctgctggac
     3181 aggtttccga cgccgaattg atcccaccat ttcgcgtcgg tccacaccgc aaagggggct
     3241 ggatcagctg ccacagctgc cataccaacg gccacacctg cgggcagttg gccgatactc
     3301 tcggcgatgg gacgttgggg acgcccaagc ggatcccaac gcttttggga acgcggctga
     3361 ccgacatgtg ggcgtggaac ggcgaagtgc gggagttgca cgagcaggtg cgaaaatcgc
     3421 tggacaacac gatgcacgcg ccgaagtaca gtttgcagga cgtgctcgac attaccgcct
     3481 ttctgcatac gctgcccccg cctccgccgc tggagccggc gaccgaggag ccggcggaca
     3541 aggtgcagct ggcccgcggc gcagggctct tcaaaacgct gggctgcgcg aagtgccatg
     3601 tgccgccgct gacctacacg tcgccggaga cgtacgacgt cggcttggag gacgaaaagg
     3661 ggctgcggaa attcaacccg ccgtcgctcc gcggcgtcag ccagggctac tcgttcttcc
     3721 acgacagccg ggcgaagaag ctggaagagg tcttcaccgt ccacggccat cagctcgacg
     3781 ggccgctgga gcaggcggag ctagccgacc tgctgcggtt tctgcggagc ctgtagctcc
     3841 gagcgtgatg gatgttgcca gacctgccgc cgcgcttgat cgccggaagc ccggaatata
     3901 taatcctgtt cataagtcga tcagcgtctg ctagcgcagc tggcacgctt gggaccggtc
     3961 gttggtggca aactgggatc tggctcggag tctgcgctta gggcatgagc agaggcaacg
     4021 tcatccttag gctgcataaa gtgcataatg catggtgcag tatgcatgga cgcaagtgct
     4081 tgatagcgaa cgggttaacg gcgaatggat ccggaatatg gcgtttgaga attgatgtga
     4141 atatgcataa acggggaacg gtcggcgggt tgccgcaatc gggttgacga aattgtcgcg
     4201 gcgtgtcatt ttgtcgtgat ccaggaattc ccggcgctcg ccaacttttg gcactgcccc
     4261 gaataccgca gcagggggca agacttgctg caagccgaat ccgctggtgc gacctgcctg
     4321 aatctgctca ccagggatgg tgcagtaggc gtcgaattta cgcccgccct ggaaccccag
     4381 cactacgccg agctgttcga gctggcccac gaattcgaat ccgaggcggc gctgcgcgag
     4441 gtggtgaccg cggccgcccg gcggtggggg cggcaggtcg tgttcggctg aggggcgaat
     4501 actcgcggtg tcgcgtcggt cggcgcgttg accctcgctg gcgcgtcttg ttagggtggc
     4561 cgggatgact ccgctggcgc gcgactttta ttcccgagat ccgattaccg tcgcccgcga
     4621 gctgctgggg agaatcctca tccgccaatg cgacgagggg cttgccagcg ggcggatcgt
     4681 cgaggccgag gcttatttga gcagccgcga cccggcttgc cacgcctacc gcggccggaa
     4741 tcgaaagaac gcgacgatgt tcggcccgcc gggacacgcg tatgtgtata tgattcacgc
     4801 ccgctggtgc ctgaacgcgg tgaccgaagc cgccgatctg ggcagcgcgg tgctgattcg
     4861 cgcggtcgag ccgttggccg gcatcgagct gatgcaagag cgccgccggc ggactgacgt
     4921 gcgcgacctg gcccgcgggc cggcccggct gtgccaggcg ctggccgtca ccaaggcgct
     4981 cgacggccac gacctgacca gcggccgcga gctgtggatc gccgccgatc cagctcaaaa
     5041 cggcgaggtg ctcgaaatcg tccgctcgcg ccgcatcggc gtcacgtccg ccgcaagccg
     5101 attgctgcgg tttttcgtcg cgggcagtcg attcgttagc ggcagagccg gtcgctcgcg
     5161 cccactcccc gcggggcggg cggcggtggt ttaatgacac ggttggtcgg ttagtcagtc
     5221 agtcggtcgg ttagtcggcg accgccacgc tgtagcgact cacggacctg ccgactgacc
     5281 gaccgaccgc ctacgggatc tctagtgctc atcctgctga ccaacgacga cgggatctat
     5341 gctccgggcc tgcaggcgct ggaggcggag ctgcggaaac tgggcgactg tgtggtcgtc
     5401 gcgccggcga cggagcagag cggcgtgggg cattcgatca cgttcctgca gccgctggtc
     5461 gccaagcagg tgttcgacgg cgatcggctg cggggctggg cggtggaggg gagcccggcg
     5521 gacagcgtga agctggggat cttcgagttc tgcccgcggc ggccggacgt cgtcgtcagc
     5581 gggcttaacg gcgggctgaa cgcgggcatc aacgtgctct actcgggcac cgtcgcggcg
     5641 gcgatcgagg gggcgttttt cgggatcacg agcatcgcct gctcgctgga gttcaacgag
     5701 cacgcgaact acctccgggc ggcgcacatc gcccgcggca tcatcgagca ggtgctggcg
     5761 cagaaaggac cggagccgca gctctacaat ctcaatattc ccacggtcgc ctgcgagcga
     5821 acggcggagg tccgcgtcgt gccgatgggc gtcgcccgct acggcgagca gttcatcaag
     5881 cgcaaagacc cgcgcgggcg gtcgtactat tgggccacca atgagccccc gccgccggcc
     5941 gaagggcatg agaccgacct gacagcgctc accaaaggat ttgtgaccct cacgcccttg
     6001 cagtacaaca tgacgaagca gccggtgctg agcgaaatgg agcgttggcg gctgagcgtg
     6061 ccacccgagt agtgaggtct ttatgaaact ctgtgttgcg attctgccac tgggtttgct
     6121 gctctgcctc gttctcttgg ggtgcgatca gcctccccaa gctggcgctc cgacggcaat
     6181 gagcgctccc ccggccccgc cgccgccacc tccgcctccg ggaacgcagc agccggccga
     6241 ggcacccgcc gctgcccccg ctgcgcctcc tggtccgccg ccgctacccg tggaggccaa
     6301 agtgggggtg tttgctggtg ggctcgacga tctggcaagg ccggtgagcc aggcttctcc
     6361 ctccgcggcg ccagcgagcc cgcctgccga ggcgaacacc gaggtcgtga aagccggcaa
     6421 gggcgtgggc ctgaagggcc ggagcctcga cccgcacgag ggcctctatg tcacgcccgt
     6481 gaaggcctat ttcgcggccc gcgaacggat cttcttcgag gccgagttcc cgacgaatta
     6541 caagctctac aacgcgtcca ccgatgacgt tcctaaggac tttgaggatt tgaaggccaa
     6601 ggtgctcgat ccctacggtc tgacgaaaaa gctgcccgtt ctgccgcagg gccacaagta
     6661 tgtgtggaac ccccagacgc aggagctggg cgtcgagcgg ccgaagcggt aataagtggc
     6721 ggcatagcgg ttgctaatta gcatggaacg aaggcaggct tctccatgcg atggcaactt
     6781 agcgttcttg cttgggcact gtccgcgtca ccggcggtcg ccgccgactg gctgaccgcg
     6841 ccctcgtatt acacgcacga cacgtccagc ggcgagcgcg tcgctcagta ctcgccgatc
     6901 ggcccgttct actattacgc ccggcccgat tacctgaaga gcggctaccg tcaatatcgc
     6961 agcacgatcg acctgggcaa cagctcggac aatttgcaca tcgtggagac ctggggcgca
     7021 cccgttcggc cctatgacga atggcgcttc ccgtatcgcc cctacagcgc accgtatccc
     7081 ggctggggtc cgccctatgg tggcctcggc ggaggcacgg gcggctatcc ctacagcggc
     7141 ggcggcggct tcggattcgg cggattcccg ttcttcccgg gcttccccgg cggcattccg
     7201 ggcggcttcc ccagtgtgac tccgcccgca gtgaactttg cccagccctg gctcgacggc
     7261 cactacccgt cgtacgactt ccgcgaccgc tcgcagtact ggcggccgta cgtcgcccca
     7321 actccaacag gtccgtagcg cgacacggca agtatattgt ctccatgacc acggagacga
     7381 aatacagcgt gcgctgctcg tgcgggcgca cgacgccggt cgagaagtcc ag
//
LOCUS       DASRJB010000418         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2373767, whole genome shotgun sequence.
ACCESSION   DASRJB010000418 DASRJB010000000
VERSION     DASRJB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2373767"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFB80_11075"
     CDS             complement(<1..820)
                     /locus_tag="VFB80_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HZN34356.1"
                     /translation="MLRWRLISAAVILAVLLTFVWLDYRQAWGAPSGAFLLPVLLVVL
                     ALATGEVLDLLAAKGHRPKAAIVYLGNVAIPLAAALPVLFGLFGRKLPAAAFPLGEFG
                     WPLVALAVATIAVLVGEMARFERPGTSIVSAALGLFTLVYVGLLGSFLALLRLFHDND
                     WGLAALFSVLLIVKMADVGAFAVGKNLGRTKLTPLLSPGKTWEGAIGGIATACLISWA
                     FFHFAAPRIVTTGYVEPPLAASLAYGLILALAGMIGDLAESLLKRDMQRKDSSSW"
     gene            complement(953..1690)
                     /locus_tag="VFB80_11080"
     CDS             complement(953..1690)
                     /locus_tag="VFB80_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZN34357.1"
                     /translation="MSDHESLADVPAERRPRHIAVIMDGNGRWAQRRQLPRIEGHRRG
                     VASVRRTVEESARLHLAQLTLYCLSSENWKRPQPELDFLMHLLEQYMIEERATIMEQD
                     ISVRVIGRRDGIPPATLAEMDKTIEMSAANRGTRLCLAINYGGRGEIVDAVRQIAAEV
                     RQGTLDPAAIDERTIDERLYSAGMPDPDLLIRTAGEMRISNFLLWQISYAELWVTEKC
                     WPEFEIADLHQAIRDFATRDRRFGGLK"
     gene            1764..2663
                     /locus_tag="VFB80_11085"
     CDS             1764..2663
                     /locus_tag="VFB80_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZN34358.1"
                     /translation="MLNRKEIVKMFRVEPDDKFRLKDHDPGWDGDDSVPKAERKELAE
                     KILSQDVSALSEAQELLYADDSRSLLVIFQAMDAAGKDGAIKHVMSGINPQGCQVYSF
                     KQPSAEELDHDFLWRCNKALPERGRIGIFNRSYYEEVLILKVHPELVAAQKIPDADPS
                     DQKFWERRYQSINNFERHLARNGTAIVKFFLNVSKDQQRQRFLDRINEPAKHWKFSAS
                     DVKERGYWDDYMCAYEECLRATSTKYAAWYVIPADKKWVTRAAVAVILTETIRGLDLK
                     WPKVTEEQKKAIAEAKRKLENES"
     gene            2682..>3085
                     /locus_tag="VFB80_11090"
     CDS             2682..>3085
                     /locus_tag="VFB80_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34359.1"
                     /translation="MERRDWLRLGTAGFAGLSLFTQTGTAQQPPAASKGGKLKITDVK
                     AILTAPANIRLVVVKVETNQPGLYGLGCATFTQRARTVEMAVKEYLRPFLLGKDPLRI
                     EDVWQSSYVSSYWRNGPVLGNALSGVDMALWD"
BASE COUNT          626 a          985 c          999 g          475 t
ORIGIN      
        1 gccaggagct gctgtccttg cgctgcatgt cgcgcttcag gagcgactcg gccagatcgc
       61 cgatcattcc ggcgagggcc aggatcagcc cataggccag gctggcggcg agcggcggct
      121 caacgtagcc ggtggtgaca atccgcggcg cggcgaaatg aaagaaggcc cacgagatga
      181 ggcaggccgt cgcgatcccg ccgatggcgc cctcccaggt cttgcccggg ctaagcaacg
      241 gcgtgagctt cgtgcggccc aggttcttgc cgaccgcaaa ggcgcccacg tcggccatct
      301 tcacgatcag cagcaccgag aagagcgccg ccaggcccca gtcgttgtcg tgaaacagac
      361 gcaagagtgc gagaaagctg ccgagcaggc cgacatagac cagcgtgaag aggcccagtg
      421 cggcactaac gatcgaggtg cccggccgct cgaagcgggc catctcgccg accagcacgg
      481 cgatcgtcgc cacagccagc gccaccagcg gccaaccgaa ttcgcccagc ggaaaggccg
      541 cagccggcag ctttcgcccg aacaatccga acagcaccgg cagtgcggcc gcgagcggaa
      601 tggccacgtt gcccagatag acgatcgccg ctttcggtcg atggcctttg gccgccaaca
      661 aatcgagtac ttcgccggtc gccagagcca gcacgacgag cagcacgggc agcaggaatg
      721 cgccactcgg cgcgccccac gcctgccggt agtcgagcca gacgaacgtc agcagcaccg
      781 ccaggatgac cgcagccgag atgaggcgcc agcgaagcac aggaagggtt cagggttcgg
      841 ggttcagggt tcagcaaagg cgaagagcat cgtacggagt ggtcagcatt tggcacagat
      901 caagcatcag ggccatcgca tgcctgaacc ccgaaccctg aactccgaac ccttacttca
      961 acccgccgaa ccttcggtcc ctggtcgcga agtcgcgaat cgcctgatgc agatcggcga
     1021 tctcgaactc gggccagcac ttctcggtga cccacagctc ggcatagctg atctgccaca
     1081 gcaggaagtt gctgatccgc atttcgcccg cggtgcgaat cagcaaatca ggatcgggca
     1141 tgccggcaga atagagccgc tcgtcgatgg tccgctcgtc gatggcggcg ggatcaagcg
     1201 tgccctgccg cacctcggcg gcgatctgcc gcacggcatc aacgatctcc ccccggccgc
     1261 catagttgat cgccaggcac agccgcgtgc cgcggttggc ggcgctcatt tcgatggtct
     1321 tatccatttc ggcgagcgtt gccggcggaa tgccgtcgcg gcggccgatc acacggacgg
     1381 aaatgtcctg ctccatgatc gtcgcccgct cttcgatcat gtactgctcc aagaggtgca
     1441 tcaggaagtc gagctccggc tgcggccgct tccagttctc gctggacaga cagtacagcg
     1501 tcagctgcgc gaggtgcagc cgggccgatt cctcgacggt gcggcggacg ctggccacgc
     1561 cgcggcggtg cccttcgatc cgcggcagct gccgccgctg ggcccagcgg ccattgccgt
     1621 ccatgatgac ggcgatatgc cgcggccgcc gctcggcggg gacgtcggcc agcgattcgt
     1681 gatcagacat acccagtctc caagcggcat tctacatcgg cggcgcgatt gttttcgccc
     1741 gccgcggaag cgataacaca accatgctca accgcaaaga gatcgtcaaa atgttccggg
     1801 tcgaaccgga tgacaagttc cgcctgaagg accacgaccc gggctgggac ggcgacgaca
     1861 gcgtacccaa ggccgagcgc aaggagctgg ccgagaaaat cctgtcgcag gatgtgtcgg
     1921 ccttgtccga agcccaggag ctcctctacg ccgacgactc ccggtcgctg ctggtgatct
     1981 ttcaggcgat ggatgcggcc ggcaaggacg gggcgatcaa gcacgtcatg tcggggatca
     2041 acccgcaggg ctgccaggtc tattcattca agcagccgtc ggccgaggag ctggaccacg
     2101 atttcctctg gcggtgcaac aaggccctgc cggagcgcgg ccggatcggc atcttcaacc
     2161 gctcgtatta cgaagaggtg ctgatcctga aggtgcatcc ggagctggtg gccgcccaga
     2221 agatcccgga cgcggatccg agcgaccaga agttctggga gcggcgttac cagagcatca
     2281 acaacttcga acggcacctg gcccgcaacg gcacggcgat tgtcaagttc ttcctcaacg
     2341 tcagcaagga ccagcagcgg cagcggttcc tggaccggat caacgagccg gccaagcact
     2401 ggaagttctc ggccagcgac gtcaaggagc gcggctattg ggacgactac atgtgcgcgt
     2461 atgaagagtg cttgcgggcc accagcacca agtacgcggc gtggtacgtg atcccggccg
     2521 acaagaagtg ggtcacgcga gccgccgtgg cagtcatcct caccgaaacg atccgcgggc
     2581 tggacctgaa gtggccgaag gttaccgagg agcagaagaa ggcgattgcc gaggcaaagc
     2641 ggaaactgga aaacgaatcg tagacaaccg gagacgactt catggaacgc cgcgattggc
     2701 tgcgactggg aacggccggg tttgctgggc tatcgctatt tactcagacc ggcacggctc
     2761 agcagccgcc ggctgcatcg aagggcggca aactgaagat cacggacgtg aaggcgatcc
     2821 tcaccgcgcc cgcgaatatc cgactcgtcg tggtaaaggt cgagaccaac cagccggggc
     2881 tgtacggtct gggctgcgcg acgttcacgc agcgggcccg cacggtcgag atggccgtca
     2941 aggagtacct gcggccgttc ctgctcggca aagacccgct gcggatcgag gacgtctggc
     3001 agtcgtccta tgtcagctcc tactggcgga acgggccggt gctgggcaat gccctgtcgg
     3061 gcgtcgatat ggccctctgg gacat
//
LOCUS       DASRJB010000419         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_967295, whole genome shotgun sequence.
ACCESSION   DASRJB010000419 DASRJB010000000
VERSION     DASRJB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967295"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFB80_11095"
     CDS             complement(<1..284)
                     /locus_tag="VFB80_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34360.1"
                     /translation="MSTSSDRLPRYTAALQFAERQVRSLIETHPDFFPMYTVRGKWKH
                     GGEKWTHWTDGFLGGMMWQFYLRSCSPPLRGGSPATESRATGDEWWRKQA"
     gene            complement(338..>2812)
                     /locus_tag="VFB80_11100"
     CDS             complement(338..>2812)
                     /locus_tag="VFB80_11100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34361.1"
                     /translation="LPAALARDVDRLQRLRGEVKNARQVAHPNVCRVYDLGEAGGQHF
                     ISMEYVDGHDLGALLKQINRLPEERGVEIARQLCLGLGAIHDRGLLHRDLKPANVMLD
                     GRGQVRITDFGLAAPAENLPAGEIRHGTPAYQAPEQLAGREVTARSDIYALGLILYEL
                     FTGKPAFAAADRMELELARAAEEPPSKPSSHISGLNPAIDKIILRCLEKEPRDRPRTA
                     YEVLSALPGGDPLAAALAAGETPSPEMVANAKTEGTLHPLVGLALSIGTIALLALGIL
                     LESHVGLFNQVPHERSPAYLAERARDLIKTLGYTDSPRDSAFGLENQPQMLKVLRERD
                     QTARRWQPLATGQPPAMFFWYRQSPRWMQAIGRNAPALPTHHGQISASNPPLTEPGMI
                     LVCLDLKGRLIELQAVPRNDTPQSAPPLDWPRLLDEAGLVNLHETKDFPPGFDPPLFV
                     ETRLAWRGSYKECPDLPVFAQAGLRGGRLVFFHAAPDHPLMRPLQLYSLSERRRTSSG
                     GAVIVAAINVAAWSLGGALAWHNLRQGRANLRGATLLAAVYVGAFFLAWLFSAHHIPE
                     FWREQELLVADLGEGLYHGLLIFVVYLAMEPFVRRRWPWRVVAWNRLLAGRWRDPLVG
                     RDILVGSAAGALAVVNFQVATLVPGWLGRPPEEPLLSYTPALTSGPAYVLASTLVFSV
                     LPTLAGFFLMFLLYLITRHEWLTAMASLLIILIPAFVARVEHRDVYVVASAVYFAGLV
                     FLYLRFGLLSMAALSVVAGIATFGPTTYRLTAWYGGESLVYFSVLLGLVVYGFVVSSG
                     GQWLAWVRHVTGEAGEEPAAASPIGG"
BASE COUNT          534 a          929 c          926 g          423 t
ORIGIN      
        1 tcggcctgct tccgccacca ttcgtcgccc gtagcccgcg actccgtcgc gggcgacccg
       61 ccacggagtg gcgggctaca gcttcgaagg taaaactgcc acatcattcc gcccaagaac
      121 ccgtcggtcc aatgcgtcca cttctccccg ccgtgcttcc acttcccacg gaccgtgtac
      181 atcgggaaaa agtcggggtg cgtttcgatc agcgagcgca cctgtcgctc ggcgaactgc
      241 agggcggccg tgtaacgtgg caaacggtcg gaagaggtgc tcatgtcgtg cggagattga
      301 ggctgatggg gctggctctg attctagccg gccggcttca gccgccaatc ggcgaagctg
      361 ccgccggctc ctcgccggcc tcacccgtaa cgtgccgcac ccaggccagc cactgcccgc
      421 cgctggacac cacaaagcca tagaccacca gccccagcag aacggagaaa tagaccagcg
      481 actcgccgcc gtaccaggcc gtaaggcggt acgtcgtcgg tccgaacgtc gcgattccgg
      541 ccaccacgct gagcgccgcc atcgacagca ggccgaaccg caagtacagg aatacgagac
      601 cggcgaagta cacggcggac gcaaccacat aaacgtcgcg gtgctcgacg cgcgctacga
      661 aggcaggaat caggataatc agcaaactgg ccatagcggt cagccactcg tgccgcgtga
      721 ttaaatagag caagaacatc aggaagaacc cggccagggt cggcagcacg gagaagacca
      781 aagtcgacgc gagcacatag gctggtccgg acgtcagggc cggggtatag ctcaatagcg
      841 gttcctccgg cggtcggccc agccagccgg ggaccagagt cgccacctgg aagttcacca
      901 cggccagcgc cccggccgcg cttcccacga ggatgtcgcg gccgaccaat ggatcgcgcc
      961 accggccggc cagcaagcga ttccaggcta cgacgcgcca aggccaccgc cggcggacaa
     1021 aaggctccat ggcgaggtac accacgaaaa tgaggagccc gtgatacagc ccctcgccca
     1081 agtcggctac cagcagctcc tgttcccgcc agaactccgg aatgtggtgc gccgaaaaca
     1141 gccaggccag aaaaaacgcc ccgacgtaga cggcggccag cagtgtggct ccccgcaaat
     1201 tggcgcggcc ctgacgcaga ttgtgccagg ccagcgcgcc ccccagcgac cacgcggcaa
     1261 cgttgatcgc ggcaacaatt accgccccgc ctgacgatgt tcttcgccgc tcgctcaacg
     1321 aataaagttg caggggtcgc atgagcggat gatcgggcgc ggcatgaaag aatacgagcc
     1381 gcccgccgcg cagacctgct tgggcgaaca caggcaagtc ggggcactct ttgtacgaac
     1441 cgcgccaagc cagccgcgtt tcgacaaaca gcggcgggtc gaagcccggc ggaaagtcct
     1501 tggtctcgtg caggttgacc aggccggcct cgtccaagag gcgcggccaa tccagcggcg
     1561 gcgcggactg gggcgtatcg ttccggggga ccgcctgcag ctcgatcagg cggcccttca
     1621 ggtccaggca gaccaagatc atgcccggct cggtcaaggg cggattgctc gcggagatct
     1681 gcccgtgatg cgtaggcaag gcgggcgcat tgcggccgat ggcttgcatc cagcgcggac
     1741 tctggcgata ccagaagaac atcgccgggg gttggccggt ggccaatggt tgccagcgcc
     1801 gggccgtctg gtcccgttcg cgcaggacct tgagcatctg cggctggttt tcgaggccaa
     1861 acgcagagtc gcgcggcgaa tcggtatagc cgagcgtctt gatcaagtcg cgggcccgct
     1921 cggccaggta agcaggcgac cgctcgtgcg gcacctgatt gaagagtccc acatgggatt
     1981 cgagcaaaat cccgagcgcc aggagggcga tcgtcccgat cgagagcgcc agcccgacga
     2041 gcggatgcag cgtcccttcg gtcttcgcgt tggcgaccat ctcgggcgaa ggtgtttcgc
     2101 cggctgcgag cgcggccgcg agcggatcgc cgcccggcag cgccgagagc acttcatagg
     2161 ccgtgcgcgg ccgatcgcgg ggttcctttt ccaggcaccg taggatgatc ttgtcgattg
     2221 ccggattcag gcccgaaata tggctcgacg gtttgctggg cggctcttcc gccgctcggg
     2281 ccagctcgag ctccatacgg tcggcagcgg cgaaagctgg cttacccgta aataactcat
     2341 agaggatgag gcccagagcg tagatgtcgc tgcgggcggt gacttcgcgg ccggcgagct
     2401 gctcgggtgc ttgataggcg ggcgtgccgt ggcggatttc gccggcgggc agattctcgg
     2461 cgggcgcggc cagaccgaaa tccgtgattc gcacctgccc acggccgtcg agcatgacgt
     2521 tcgccggctt gaggtcgcga tgcaagaggc cgcggtcgtg gatcgcgccc aggcccaggc
     2581 agagctgccg cgcgatctcg acgccgcgct cttccggtaa gcggttgatc tgcttcagca
     2641 gcgcgcccag gtcgtgacca tcgacgtact ccatgctgat gaaatgctgc ccgccggctt
     2701 cgcccaggtc gtacacgcgg cagacgttgg gatgcgccac ctggcgggcg ttcttcacct
     2761 cgccgcgcag gcgctgcaag cggtcgacgt cgcgggccag cgccgcgggc ag
//
LOCUS       DASRJB010000420         7175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3782748, whole genome shotgun sequence.
ACCESSION   DASRJB010000420 DASRJB010000000
VERSION     DASRJB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782748"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFB80_11105"
     CDS             complement(<1..696)
                     /locus_tag="VFB80_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34362.1"
                     /translation="MAKAAAGKQVNVAIIGLGFGAEFIPIYQAHPSANMYAICQRNEA
                     KLNKVGETFGIGTRYAEYDDVLADPKVDFVHINSPIPDHAWMSIAALKAGKHVMCTVP
                     MATTIKECDKICQLVADTGLTYMMAETVVYSREFLFIKQMYQKGELGKIQHLAASHPQ
                     DMDGWPDYWERMIPMHYATHVVSPCLGLLDKRAEYVSCFGSGTVRKDIAKKSGNKYAV
                     ESCHIKVQDSDVTA"
     gene            complement(831..1790)
                     /locus_tag="VFB80_11110"
     CDS             complement(831..1790)
                     /locus_tag="VFB80_11110"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34363.1"
                     /translation="MSRRRVRKLGFTLVELLVVIAIIGVLVALLLPAVQAAREASRRT
                     KCSNHLRQQALACHTHHDSLGFFPTAGRQDYTQGRDAANPFATGVAQRWHWRYQILPY
                     LEQENTFRIATDSVIRVTAVPTYNCPSRRPPTLVGAVLLVDYAGNGGTSWCPADRQDT
                     WNGTIVPSSLNNNTPWRLCRIADVTDGTSNTLLLGEKFVPVDHYRTALAWGDNEPWLQ
                     GNRWIHTRHAIHQPRMDIRATTATQEVVPPTAPTPGFCGPWHGAPNTGGGYYDYWGSA
                     HPGGFNGALADGSVRVIRYNLALNVLNALSDRADGTAFDSSGL"
     gene            complement(1822..2301)
                     /locus_tag="VFB80_11115"
     CDS             complement(1822..2301)
                     /locus_tag="VFB80_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34364.1"
                     /translation="MLVGCRRCYCGRCRYGALVLLAAILLVGLAGCRPAVDVVSLSKA
                     EQELTYIAMAYSDAHTKLGHGPKDAEELKPFLKVFGDPEDLLISPNDKQPYVIVWNVN
                     PTGGPTEYQGMFPILAYEKQGTGGRRAITDVRGRPMTVPNEDLSKLTYVGRHKPATN"
     gene            2503..3627
                     /locus_tag="VFB80_11120"
     CDS             2503..3627
                     /locus_tag="VFB80_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HZN34365.1"
                     /translation="MRPSKHVALLVETSNAYARGILRGVMAYVREHKPWALRLTEHGR
                     GEMRRNELAEWTGHGIIARIENPRIAAALKNCKCPVVDVSAARLLPKLPWVETDDAAI
                     ARAAAEHLLSRGLTHFGYCGDDRFNWSRWRREAFHQCLAEAGFTCHDAPQGRGDQFAA
                     WVRSLPKPCGVMACYDIRGRELIDGCRVAEVEVPDELAVVGVDDDELLCELCDPPLSS
                     VIPDVRRTGWVAAEILDAWMSGRPPRKLNHPIPPLGVTTRRSTDMLAIEDGDLAAAVR
                     FIRQRACSGITVDDVVSQVPLSRRVLESRFERLLGRSPHAEILRVRLERVKQLLTQPG
                     WTLDAIAHRTGFRHGAYLSAVFKREFGLTPGAYRRQHQLD"
     gene            3796..4941
                     /locus_tag="VFB80_11125"
     CDS             3796..4941
                     /locus_tag="VFB80_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34366.1"
                     /translation="MPIASHRYSGRPAFTLVEMLVVIAVIGILIALLLPALHLAREVA
                     RGMACSSNLRQFGVGFHTHAEQHRDAYCSGAFDWRKDGAITEFSWVADLVKQAQPVGK
                     MLCSSNPARGADVLNELLSCNATGFASNSCVNMLGPPPSKAPDGSDVYNPCRWIADSK
                     SGFAAGPSPARTAYVEEYVVQEFYNTNYTASWWLVRSEVRLNAYGNLRPAISGCGNAI
                     DGRNVTAGPLHRTTMDISSTPSSNVPLLGDGGASGENLEYPLAGLSSGSPLVRSLTKG
                     PVLIANCPNGQAFNPPAFAEPNAGKSVWWSVWTQQTLQDYRNFGVPHRGTCNVLFCDG
                     SVRSVADPNKDDQLNNGFPAGGGFADNTVEWPKDEVFSRYALDYKKL"
     gene            5004..6314
                     /locus_tag="VFB80_11130"
     CDS             5004..6314
                     /locus_tag="VFB80_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34367.1"
                     /translation="MKKRGFTLIELLVVIAIIGVLVALLLPALAVAREAARNASCRNN
                     LRQFGIAMHAFADKDPSGRYCTGASDFRRDGCMDTYGWVADIVNQGAGKVSTMLCPSN
                     PLVASEKVNDLYGKDTTDAKDACPPARLATGFCGQTGIGGIFSDGAGGFTKTAAGTFA
                     NTAINTPERATAIAWGLFENGYNTNYSASYYLVRTIPRTINDPASPSTNVPFTDWITA
                     GPFATSAKGLGGSLGPLTRRVAESGQIPTSNIPLLGDAAPGDVDEAIMAATIMRSDND
                     FVGQSMNSSAAAKGERVYVPSGSVLTEAFNDGPAFYNSAAFNLDLIAVNRTLGVQLAA
                     ELAGTISPPPTTGSNTYLQDTRDWFTVHGGRSGSCNILMADGAVKTFYDSNGDKFLNP
                     GFPVPQLTPSQVLTCGYSDGTIELPPGEIFSGLFLAKQPKAKFE"
     gene            6417..>7175
                     /locus_tag="VFB80_11135"
     CDS             6417..>7175
                     /locus_tag="VFB80_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HZN34368.1"
                     /translation="MWRTFWIVAGCIGAIIGGLVWHSATQATFLFHHPRTASVRVEID
                     RRTGAAVVRPKAQAAVAVAAVAPGAQPRVVAEHRLHDFGTMDPLTMGSHEFVVKNSGA
                     APLALRVGPTTCKCTVSSLAQSTVPPGGSTTVRLEWNTGRDERYEHAGTIFTNDPTQK
                     SIDLRVVGKVRLLIGCDQKEIVLEALNPSAPAVIERVIYSQTLDAFTITRLDSTISGL
                     TWSAEPADPATLPALSPKHAQRLRIAIPSGSLTGI"
BASE COUNT         1254 a         2420 c         2263 g         1238 t
ORIGIN      
        1 ggccgtcacg tccgagtctt ggaccttgat gtggcagctc tccacggcgt atttgttgcc
       61 cgatttcttg gcgatatcct tccgcaccgt cccgctgccg aaacacgaga cgtactcggc
      121 ccgcttgtcc aacaggccca gacacgggct gacgacgtgc gtggcgtagt gcatcgggat
      181 catgcgctcc cagtaatcgg gccagccgtc catgtcctgc gggtgcgacg ccgccaggtg
      241 ctgaatcttg ccgagctcgc ctttctgata catctgcttg atgaacagga attcgcggct
      301 atagacgacc gtctcggcca tcatgtaggt caggccggtg tcggccacga gctggcagat
      361 cttgtcgcac tccttgatgg tcgttgccat cggcacggtg cacatcacgt gcttgccggc
      421 cttgagggcc gcaatcgaca tccaggcgtg gtcggggatc ggcgagttga tatggacgaa
      481 atcgaccttg gggtcggcca gcacgtcgtc gtattcggcg tatcgcgtgc cgatgccgaa
      541 cgtctcgccc accttgttca gcttggcttc gttccgctgg cagatcgcgt acatattggc
      601 gctgggatgc gcctgataga tgggaatgaa ctccgcccca aaccccagcc cgatgatcgc
      661 gacgttgact tgcttgcccg ctgctgcttt ggccatgaca gaagggtgtt gcccgcaaag
      721 gtgatggact agccgggacc aaacctggtc ccggagtgcg attgcacgcg gcgtattcta
      781 atcacccgcc gcgctccatc acaccaccgc cgacatggcc actcgcgttc ctacaagccg
      841 ctcgagtcga acgccgtccc gtccgcccga tccgacaggg cgttgagcac gttcagtgcg
      901 aggttatacc gaatcacccg caccgaccca tcggccaggg cgccattgaa gccgccggga
      961 tgggccgagc cccagtagtc gtagtagccg ccgccggtgt tcggagctcc gtgccacggc
     1021 ccacagaagc caggggtcgg agcagtcggc ggaacgacct cctgcgtcgc agtcgtcgcg
     1081 cgaatatcca tgcgcggctg atggatggca tgccgggtgt gaatccagcg gttaccctgc
     1141 agccacggct cgttgtcgcc ccaggcgagc gccgtgcggt agtgatccac gggcacgaac
     1201 ttctcaccca acaacaacgt gttcgatgtg ccgtcggtca cgtcggcgat ccggcagagt
     1261 cgccagggcg tgttgttgtt gaggctgctc ggcacgatcg tgccgttcca ggtgtcctgg
     1321 cgatcggccg ggcaccacga cgtgcccccg tttcccgcgt aatccaccag gaggaccgcc
     1381 cccaccaggg tcggcggccg gcgcgagggg caattgtacg ttggaaccgc cgtcacacgg
     1441 atcacggagt cggtggcgat acggaaagtg ttttcctgtt ccaggtaggg caggatttga
     1501 taccgccagt gccaccgctg ggccacacca gtggcgaacg ggttggcagc gtcgcgccct
     1561 tgtgtatagt cctgccggcc ggccgttggg aaaaagccga gcgaatcgtg gtgggtatgg
     1621 caagccaggg cctgctggcg caggtggttg ctgcatttgg tacggcggct cgcctcgcga
     1681 gcggcctgca cagccggcag caagagcgcc accaatactc caatgatggc gatcaccacc
     1741 agcaactcga cgagcgtaaa acccagcttt cggacacgac gcctggacat ggcaaacctc
     1801 cgaatcagca aacggtttgt atcaattggt cgccggcttg tgacggccga cgtaagtgag
     1861 cttggaaaga tcttcgttgg gaacggtcat cgggcgcccg cggacatccg taatggctcg
     1921 cctgccgccc gtgccttgct tctcgtaggc caggatcgga aacattccct ggtactcggt
     1981 cggcccgccg gtgggattga cgttccacac gatgacgtag ggctgcttat cgttgggcga
     2041 gatcagcaag tcctccgggt cgccaaacac cttcagaaac ggcttcagct cctcggcgtc
     2101 cttcggccca tggccgagct tcgtatgcgc gtcggaatag gccatcgcga tgtaggtgag
     2161 ctcttgctcg gccttagaca gcgagaccac atccaccgcc ggacggcagc cggcgaggcc
     2221 aaccaagaga atggcagcca acagcacgag cgccccatag cggcaacggc cgcaatagca
     2281 acggcggcaa cccacgagca tggcgactcc cttccgcgac aaggacggtg gaaggatagc
     2341 ccggacccaa acgcaatctg gacgctgttc attctcccgc gcggcaagcc gcccgccatg
     2401 cgataatgcg cagaacttat tagacttttc accgcgcggc tggacgccgg cgggcgattt
     2461 gcgaagcatt agcggctaga tcgccggggg gcgggggtgg gaatgcggcc gagcaagcac
     2521 gtcgcactgc tggtggaaac gtcgaacgct tacgcccgcg gcatcctccg cggcgtgatg
     2581 gcctatgtcc gcgagcacaa gccttgggcc ctccgcctga ccgagcacgg ccgcggcgag
     2641 atgcgccgca acgagttggc cgaatggacc ggccacggca tcatcgcccg catcgagaac
     2701 ccgcggatcg ccgcggccct gaagaactgc aagtgccccg tggtggatgt ctccgcggcc
     2761 cgcctgctgc cgaagctgcc ttgggtcgag accgacgacg cggcaatcgc gcgggccgct
     2821 gccgagcacc tgctctcccg cggcctgacg cattttggct attgcggcga cgaccgcttc
     2881 aactggtcgc ggtggcggcg cgaggcgttt caccagtgcc tggccgaggc cggcttcacc
     2941 tgccacgacg cgccgcaggg ccgcggcgac cagttcgccg cctgggtccg ctcgctcccc
     3001 aagccctgcg gcgtcatggc ctgctacgac atccgcggac gcgagctgat cgacggctgc
     3061 cgcgtcgcgg aggtcgaggt gcccgacgag ctggccgtcg taggcgtgga cgacgacgag
     3121 ctgctctgcg agctgtgcga tccgccgctc tccagcgtga ttcccgacgt ccgccgcacc
     3181 ggctgggtcg cggccgaaat cctcgacgcc tggatgtccg gccgcccgcc gcggaagctg
     3241 aaccatccga tcccgccgct gggggtcacc acgcggcgct cgaccgacat gctggccatc
     3301 gaagacggcg acctggcagc agccgtccgc ttcatccgcc aacgggcctg ctccgggatc
     3361 accgtcgacg acgtcgtttc ccaagtcccg ctctcgcggc gcgtcctgga gtcgcggttc
     3421 gaacggctcc tgggccgcag cccgcacgcc gaaatcctgc gggtccgcct ggagcgggtc
     3481 aagcagctgc tcacccagcc cggctggacg ctcgacgcca tcgcccaccg caccggcttc
     3541 cgccacggcg cgtatctaag cgccgtcttc aaacgcgaat tcggcctgac cccaggcgcg
     3601 tatcgccgcc agcaccagct cgattagcgt gccgggctgc agccaaatcg gggcgctgtt
     3661 ccttaacaca agtcgcgcgc aaagttcact gcggcgtcac accaacttca tttgcctcgc
     3721 ggaatttatt gccatttgct tgaccgcacg caatcggcgc ggcgagcagg gaagtttcgc
     3781 ctggcggggg accaaatgcc gatcgcttcg caccgctact ccggtcggcc ggcgtttacg
     3841 ctcgtggaaa tgctcgtggt gatcgcggtt atcggaatct tgatcgcgct cttgttgccg
     3901 gcgctgcacc tggctcgcga ggtcgcccgc ggcatggcgt gcagcagcaa cctgcggcaa
     3961 ttcggtgtgg gctttcacac gcacgccgag caacaccgcg acgcctactg ctcgggggcc
     4021 ttcgactggc ggaaggacgg cgcgatcacg gaattcagct gggtcgccga cctggtgaag
     4081 caggcgcagc cagtcgggaa gatgctctgc tcgtcgaacc cggcccgcgg cgccgatgtg
     4141 ctcaacgagc tgctcagctg caatgctacg ggcttcgcct ccaactcgtg cgtgaacatg
     4201 ctggggccgc ctcccagcaa agcgccggac ggcagcgacg tctataaccc ctgccgctgg
     4261 atcgccgact ccaagtcggg cttcgcggcc ggtcccagcc cggcccgcac ggcgtatgtc
     4321 gaggagtacg tcgtgcagga gttttacaac accaattaca ccgcctcgtg gtggctggtc
     4381 cgcagcgagg tgcggctgaa cgcctacggc aatctgcggc cggcgatttc cggctgcggc
     4441 aacgccatcg acggccgcaa cgtgacggcc ggcccgctcc accggaccac gatggacatt
     4501 tcctcgacgc cctccagcaa cgtaccactg ctcggcgacg gtggggcgtc cggcgagaac
     4561 ttggagtatc cgctggcggg tttgtcgtcg ggatctccgc tggtccgttc gctgacgaag
     4621 gggccggtgc tcatcgccaa ttgtcccaac gggcaagctt tcaacccgcc cgccttcgcc
     4681 gagcccaacg cgggcaagtc ggtgtggtgg agcgtgtgga cgcagcagac gctgcaggac
     4741 tatcgcaact ttggcgtccc ccaccgcggg acctgcaacg tgctgttctg cgacggcagc
     4801 gtgcggtcgg tcgccgaccc caacaaggac gatcagctca acaacggatt tcccgccggc
     4861 ggcggattcg ccgacaacac ggtggaatgg ccgaaggacg aggtcttcag ccgctacgcg
     4921 ctggattaca agaagctctg agcggctgcg cgattcacgg tttgcggtac tagttcactc
     4981 ttttcacttg aggagcaagg atcatgaaga aacgcggttt cactctcatc gagctgctgg
     5041 tggtgatcgc catcatcggc gtgctcgtgg cgctgctgtt gccggctttg gcggtcgccc
     5101 gcgaggcggc ccgcaacgca tcgtgccgca acaacctgcg gcagttcggc atcgccatgc
     5161 acgcctttgc cgacaaggac ccgtcgggcc gctactgcac gggcgcgagc gacttccgcc
     5221 gcgacggctg catggacacg tatggctggg tggccgacat cgtcaatcag ggcgcgggca
     5281 aagtcagcac gatgctctgc cccagcaatc cgctggtcgc atcggagaag gtcaacgacc
     5341 tctacggcaa ggacacgacc gacgcgaagg acgcctgccc gccggcccgc ctggcgacgg
     5401 ggttctgcgg ccagaccggc atcggcggca tcttctcgga cggcgctggc ggcttcacca
     5461 agactgctgc cggcaccttt gccaacaccg cgatcaacac gcccgagcgg gccaccgcga
     5521 tcgcctgggg cttgttcgag aatggctaca acacgaacta ctccgccagc tattacctcg
     5581 tccgcacgat tccgcgaacg atcaacgacc cggccagccc ttccacgaac gtgccgttca
     5641 ccgattggat cacggccggc ccattcgcca cttcggccaa gggcctgggc ggctcgctcg
     5701 gcccgttgac ccgccgcgtg gcggagtcag ggcagattcc gacctcgaac attccgctgc
     5761 tgggcgacgc ggcgccgggc gacgtcgatg aggctattat ggccgcgacc atcatgcgct
     5821 ccgacaacga cttcgtcggc cagtcgatga actccagcgc cgcggcgaag ggcgagcgtg
     5881 tttacgtgcc ctccggctcg gtgctgacgg aagccttcaa cgatggcccc gcgttctaca
     5941 actcggccgc cttcaatctc gacctgatcg ccgtcaaccg gacgctgggc gtgcagctgg
     6001 ccgccgagct ggcggggacg atctcgccgc cgcccaccac gggcagcaac acctacctcc
     6061 aggacacccg cgactggttt acggtgcacg gcggtcgcag cgggagctgc aacatcctga
     6121 tggccgacgg cgccgtcaag acgttctacg actccaacgg cgacaagttc ctcaatccgg
     6181 gattccccgt gccgcagctg acgccgtcgc aggtgctgac ctgcggctac tcggacggca
     6241 cgatcgagct gccgccgggc gaaatcttca gcggcctgtt cctggccaag cagccgaagg
     6301 cgaagtttga gtaacacggg cgcaaagatt cggaatgcag gatcgaacct cgatcctgca
     6361 ttccgaattc tcaatctaag ttctgaattc tgcattctga attctgaatt ccaaacatgt
     6421 ggcggacgtt ttggatcgtc gcgggctgta tcggcgcgat tatcggcgga ctggtttggc
     6481 acagcgcgac gcaggccacg ttcctgtttc atcacccgcg gaccgcaagc gtccgcgtcg
     6541 agatcgaccg ccgcaccggc gccgccgtgg tcaggccgaa ggcccaggcc gcggttgcag
     6601 tagccgcggt cgcccccggc gcacagccgc gagtcgtcgc tgagcaccgg ctgcacgact
     6661 ttggcacgat ggacccgctg accatgggca gccacgagtt cgtcgtcaag aacagcggcg
     6721 ccgccccgct cgcgctccgc gtcgggccca cgacctgcaa gtgcacggtc agcagcctgg
     6781 cacagagcac cgtgcctccg ggcggctcca cgacggtccg cctggaatgg aacaccggcc
     6841 gcgacgagcg ttacgagcat gcgggcacta tctttaccaa cgaccccact caaaaatcga
     6901 tcgacctgcg ggtcgtcggc aaagtccgcc tgctcatcgg ctgcgaccag aaggaaattg
     6961 tgctcgaggc gctgaatccg tcggcgcccg cggtcatcga gcgggtgatc tactcgcaaa
     7021 cgctcgatgc gttcacgatc accaggttgg attcaaccat tagcgggctg acctggtccg
     7081 ccgagcccgc cgatccggca acgctgcccg ccctttcgcc aaagcacgcc cagcggctgc
     7141 gaatcgccat ccccagcgga tcgctgacgg ggata
//
LOCUS       DASRJB010000421         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_400302, whole genome shotgun sequence.
ACCESSION   DASRJB010000421 DASRJB010000000
VERSION     DASRJB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400302"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFB80_11140"
     CDS             complement(<1..485)
                     /locus_tag="VFB80_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34369.1"
                     /translation="MAIKAGIAARTLLAALLVAAAPAAADDGFFRAALVAGGTDDACE
                     ADHWQQVYVELRGAILTAELIRLPQAARLAAVQRELHEQILIGSYQPAASDLRLALGR
                     GDYNCLSAAALGFDLCRAAGIELEIWSRPGHVWLLTAEGTAIEPASLPTSTPSLALRV
                     GV"
     gene            731..1603
                     /locus_tag="VFB80_11145"
     CDS             731..1603
                     /locus_tag="VFB80_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34370.1"
                     /translation="MRMIPTAAILLLVAAAATAAETSVSGTFKGNGKDAKIAHVSVQR
                     REPFSDKASLRLIFTEKDHTRDPKADWNASFGRYGSALVISLFEADGGIFGCEVSHAA
                     HSKGTFSSIGKIEVADYKNEGGQITARLTTGGQEDTFGQTWEVDLKFSVPALPPEAKP
                     ARPLKIVKSEERGPVEPKSKPARAEKPAEGGPALNVRDLPLPEGASEVTYKKLVEMIT
                     YKCPSDVKTTATFLMEKLAAGGWDKSGPDLVTPASAIIARKRGGATLRLIITPSDDGS
                     EVKLFTTGLAWDEK"
     gene            complement(1572..2426)
                     /locus_tag="VFB80_11150"
     CDS             complement(1572..2426)
                     /locus_tag="VFB80_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34371.1"
                     /translation="MSLPHRPRWPRPQFSLRLLLAALTTCCVVAAAWFRWPYAETTVP
                     SQKPLFAGDCGTPIYDADCEYSRVTRHWLRQWGGGRVQHGWERKYATAGWLAVRQQYQ
                     AGQPHGLYQRLNSAGQVIESGRYHHGRRDGVWTVLRADCSYRMQFSQGRRHGEFVASS
                     RWIKRSLLFENDRLAAIDGEPVAPVPRGDLAVLDRELPPMEFVETPLREVCDVLSEMY
                     TVPISLDREQDPQLPISQSPWPFSQTLGEGLAIIAQTHGLTWRCEGDGICFSSAQSPT
                     SHPTPDRS"
BASE COUNT          449 a          804 c          821 g          375 t
ORIGIN      
        1 acaccaaccc gaagcgccag cgagggagtg ctggttggca gcgacgccgg ctcgatcgcc
       61 gtcccttccg ccgttagcag ccacacgtgc cccggccgcg accagatctc cagctcgatt
      121 ccagccgcgc ggcacagatc gaaaccgagc gccgccgccg agaggcagtt gtaatcgccg
      181 cggcctagcg ctagccgcag gtcgctcgcc gccggctggt agctgccgat gaggatttgc
      241 tcgtgcagtt cccgctggac tgccgccagc cgcgccgcct gcggcagtcg gatcagctcg
      301 gccgtgagga tcgctccgcg cagctcgaca tagacctgct gccagtggtc ggcttcgcag
      361 gcgtcgtcag ttccgccggc gacgagcgcc gcgcggaaaa agccatcatc cgccgcggcc
      421 ggcgcggcgg ccacgagcag cgccgccagc agcgtgcgcg cagcaattcc agccttgatc
      481 gccatccgca atcccgcctg gggagaatcg acgagccatt ctgcgctctg gattgggtgg
      541 cggccgcagc ggcgcggtgg gcgaaggtga acgggcctgc atgagggccg gttatgcggg
      601 tttggcggaa ttcgccggca agcgatttct ggccagtttc gcggcgtcgc gtatgcttgc
      661 cgatccggtt cagggccaag gaccacgaaa tacacgccgc acacgaaaag gaagaagagg
      721 aagtatgacg atgcgaatga tccccaccgc cgccattttg ttgctggtgg ctgccgccgc
      781 gacggccgcc gagaccagcg tctcgggcac gttcaagggc aacggcaagg acgccaagat
      841 cgcccacgtt tcggtgcagc gccgcgagcc gttcagcgac aaggcgtcgc tgcggctgat
      901 cttcaccgag aaggaccaca cccgcgaccc gaaggcggac tggaacgcct cgttcggccg
      961 ttacggcagc gcgctggtga tctcgctgtt cgaagcggac ggcggcattt tcggctgcga
     1021 agtgtcgcac gccgcgcaca gcaagggcac gtttagctcg atcggaaaga tcgaggtggc
     1081 ggactataag aacgaagggg gccagatcac tgcccggctc acgaccggcg gccaggaaga
     1141 cacttttggc cagacctggg aggtcgatct gaaattcagc gtgcccgcgc tgccgccgga
     1201 agccaagccg gccaggccgc tgaaaatcgt caaatccgaa gagcgagggc ccgtcgaacc
     1261 caagagcaag ccggcgcgtg ccgagaagcc ggccgagggc ggcccggcgc tgaacgtccg
     1321 tgacttgccg ctgccggaag gggcgagcga ggtcacgtat aagaagttgg tcgagatgat
     1381 cacctacaag tgtccgtcgg acgtgaaaac aacggcgact ttcctgatgg agaagctcgc
     1441 cgccggcggc tgggacaagt ccggccccga cctggttacg cccgcctcgg ccattatcgc
     1501 ccgcaagcgc ggcggggcga cgctgaggct gatcatcacc ccgtccgacg acgggagcga
     1561 agtgaagctc ttcacgaccg gtctggcgtg ggatgagaag taggcgactg ggcagagcta
     1621 aagcagatgc cgtccccctc gcaccgccac gtcaggccgt gcgtctgcgc gatgatcgcg
     1681 aggccctcgc cgagcgtttg tgaaaacggc cacgggctct gggaaatggg cagttgcgga
     1741 tcctgctcgc gatccagcga gatgggaacc gtatacatct cgctcaggac gtcgcacacc
     1801 tcgcgcagcg gcgtttcgac aaactccatg ggtggcaatt cgcggtccag caccgccagg
     1861 tcgccccgcg gcactggagc cactggttcg ccgtcgattg ccgccaggcg atcgttttcg
     1921 aacagcagcg agcgcttaat ccatcgcgag ctagccacaa actcgccgtg ccgccggccc
     1981 tgggagaatt gcatccgata ggagcagtct gccctgagca cggtccagac gccgtcccgc
     2041 cggccgtggt gatatcgtcc agattcgatg acttgccctg ccgagttcag ccgctggtag
     2101 aggccgtgcg gctgtccagc ctggtattgc tgccgcaccg cgagccagcc ggctgtcgcg
     2161 tatttccgct cccagccgtg ctgcacgcgg ccgccgcccc actgccgcag ccagtggcgc
     2221 gtcacccggc tgtactcaca gtccgcatcg taaatagggg tcccgcaatc gccggcgaag
     2281 agcggcttct gcgacggaac ggtcgtttcc gcatacggcc agcgaaacca ggccgcggct
     2341 acgacgcagc acgtggtgag cgcggccagc agcagccgca ggctgaattg gggtcggggc
     2401 cagcggggac ggtgcgggag gctcaaggtc tttactccgg atcgctacg
//
LOCUS       DASRJB010000422        11990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2374028, whole genome shotgun sequence.
ACCESSION   DASRJB010000422 DASRJB010000000
VERSION     DASRJB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374028"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VFB80_11155"
     CDS             <1..1283
                     /locus_tag="VFB80_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34372.1"
                     /translation="KRLLLSSTRITDAGLVHLSGLSNLVELALADNPISDAGLARLRG
                     LVKLTELDLERTAVTDLGLPALAEMRRLETLNVRRTKVTTPGLRQLAGLTELRRLWAG
                     ESLLRSAPPARQWQVWLASAYGDDIYIYELGSFQLLKRIQVGPNPHGLSATADGRTVH
                     VALEHFDDPVGELAWIDTRSFDVTAKVKVGPQPQEIDCTPDGKWIYVPCADGQWHVVD
                     GHERRVVTTIHTGGRPHNTVASPDGKRMYLSPMGYPQAVTVVDVAAGHRVIGEIPFQD
                     VTRPPAITADERLFFQNIDNMLGFQAADIAQRRVIKTIEHSIPPQRRGEWSRSHGLGV
                     RPDQQEIWSCNVEHHLVHVHEMSSGNYREIASITLPGPVYWLSFSPDSKYCFVSVRSK
                     RQVAVIDCISKNIVTMLDAGQAVKRTQVVGVPLD"
     gene            complement(1296..2495)
                     /locus_tag="VFB80_11160"
     CDS             complement(1296..2495)
                     /locus_tag="VFB80_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN34373.1"
                     /translation="MNCIRALGLLLLLVPGGLALADDEATKPKPLDEVAPRPKPVEAP
                     TRQALDEAIRRGVDFLLADQNKDGSWGSAERTKDLNIYAPVPGAHHAFRSAVTAMCVS
                     ALLEVEDRRPEVSTAIDGGEKWLLENLPHLRRAEPMAIYNVWGHAYGISGLVRLYKHR
                     SGYTDKQAEIKKQIEQQIELLGRYETANGGWGYYDFNAQTKKPSGEPTSFTTATVLVA
                     LHEATEIGIQVPQKLIDRGLATIRRQRKPDGSYVYDDYLLLMPQHPVNLPGGSSGRSQ
                     ACNYTLRLYGDKRITDAVVRAWLDRLFARGMWLDIGRKRPIPHESWFAIAGYFFYYGH
                     YYAAKCIEELPESDRAEMRGQLAHRLLPLQEKDGSWWDYPLYNYHQQYGTAFALMSLQ
                     YCRASAK"
     gene            complement(2567..4366)
                     /locus_tag="VFB80_11165"
     CDS             complement(2567..4366)
                     /locus_tag="VFB80_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZN34374.1"
                     /translation="MVNRALIRNLENDPEIESMFAAATATFEEAGLFQESGGGSEFEA
                     GKIVEGRILRVEDGMVMVDIGFKSEGSVPADEWREGEEPPTVGMLVKVLIQDLEDEQA
                     GPDDGGMVRITKNGADRLIQWNEVISKLKEGDPVTGVVERKIKGGLLVDIGGVKVFLP
                     ASQVDIRRPSDIGDYIGRTVQCEVLKIDEARRNIVVSRRSLIERQREEDREALLKELE
                     VSQVRKGVVKNIADFGAFVDLGGIDGLLHITDMSWERIGHPSEMVAIDQEIEVMVLHI
                     DREKKKIALGLKQKGASPWDNVEEKYPVGSSHKGSVVNVMSYGAFVKLEPGIEGLVHI
                     SEMSWTRRVNHPSELVNIGDEISVVILGVDKAGQQLSLGMKQTQENPWTRVAEKYPVD
                     KIVEGKVRNLTNYGAFVELEEGIDGLLHVSDMSWTRKISHPNEMLEKGQAVRCKILSV
                     DQERRRIALGMKQLDDDPWNTNIPAKYQPGQIVSGTVTKITNFGVFVGLEDGLEGLLH
                     ISELADHKVENPEDVVKVGEKIDVKILRVDTDDRKIGLSRKRVEWAEDDLSGAPNINA
                     DGTPAAPPKEKKPRGELKGGLGGSGPLISPPTQ"
     gene            4594..6843
                     /locus_tag="VFB80_11170"
     CDS             4594..6843
                     /locus_tag="VFB80_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34375.1"
                     /translation="MGIAAFTGKLACGLLLAAALASSGPGQERQPLPTPRQMADSRED
                     LWAEAAIKQPGGPSYEFFAPLLPPLRYVNTAFRHYPIVLSAPGAPVKARWVSDGSGVN
                     LRADKPPMWREIGTPVEFFVGADRKPFGADPAQLSEPALEDGYLPILQLQYEHAGTKY
                     DQQALAVTRGAASEYGAVLVRFRCPGKAGIVSARIGGDDRLTVKDSRIENAAGQSLTS
                     FGPPWRWDSDRRSLVASLAAEAAATLLIATQPSPEPLPDKSWNAAHAEAVADWRAILA
                     RGTQLTIPEPLVQDAWRALVLGNYAIASGDQMNYSAGNAYDHLYEGECGDAARSLMLF
                     GQQSDARRMIGPLLDFQRQATRFHVAGHKLQLLTHYYWVTRDAEYLREKRGVWEPVAS
                     FIRTSRQPEHGLLPKDNYAGDIPQQVWSLSSNSNCWRGLRDLGAVLEDCGEKSLAAEL
                     RREATEYRAAILAAVEKSINRSTQPPFIPIALLGDEPAHDPLTATRMGSYYDLMTPYV
                     LGSGVFRPGDQREQWLIDYFRRHGGLAMGMIRSQPHQGEFQGQPGVNVLYGQRYMLTL
                     LRRGDREHALAGFYGHLAQGMTRGTFIGGEGSRFFHGDERGRSFYLPPNSASNAMFLT
                     TLRYLLVQDWDLDDDGEPETLRLFDAIPPRWLADGQTLAAQRLPTAFGELSFRAVSQF
                     ADRRLHVTLELPPRPAERVTLRLPNPPGHKIEAAQMGGRTIAPDAEGRFDLRGAAGRS
                     ELVVTVRKD"
     gene            7029..8735
                     /locus_tag="VFB80_11175"
     CDS             7029..8735
                     /locus_tag="VFB80_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN34376.1"
                     /translation="MKLLQLLVLILLGGVFWCGCQWPATPQWVSSSRTMPWARIQNYA
                     ESSSAPRSATASSGRLPAKPQMPAWIYLAFIVGSSVFLSLVDRWRVEIARRFARENHL
                     ALPRIVTQPLVLRRHLLLALAGCIALAVYGSLVPFHARPQTWPAALREFGDGLVRPLS
                     FGSKTDWATNVLLFVPIGFLATGAALGRVASDRRRALLPPAIVAGCTLLSLAVEFGQL
                     WVADRFCSQNDIVAESLGGLVGVSLWLVSGSTVATWLDAFRTQRRPRERLERLLQVYL
                     GAVLVWVVLPLDLTLRPAEIWDKFQAGRINLIPFSDLQWTTAGAVGWLEKLLLLLPLG
                     ALAALWRWPRADSVRPLGRALLLGAGLIASLEACQLLVFSRTCSTTDVVSGLAAIAAG
                     WALARWRLHPAAIDSGPRSGWLALAVVAVGFWAALAAVFCLPFDRVTSSAEAAQRWQA
                     LWSTPMLATFQSGAISNALAEALRKTLLFGTSGALVGLLARPFAPGSPQRTPAILCAM
                     GLQIALPLAIEFAQLWLPPHQPELGDVLLGTIGGGLGMVGVLTADRDLSIAAPDLKDG
                     RR"
     gene            8956..9252
                     /locus_tag="VFB80_11180"
     CDS             8956..9252
                     /locus_tag="VFB80_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34377.1"
                     /translation="MTKSPRRSRSKSPAGGLAGITLSELHARRVAIHLVALGREQFIR
                     GQGQFEVDPDLGRVLRVKVKDPDGDFELVVSEAKWNGQVLPGQAVGCDYLLRLG"
     gene            complement(9265..>11990)
                     /locus_tag="VFB80_11185"
     CDS             complement(9265..>11990)
                     /locus_tag="VFB80_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN34378.1"
                     /translation="GSSVLLTSSPSNSLGASFSTTVAAGTYYLVARSRGDYGDMGQYT
                     ITGSVPVGPPPEIEVTAGGAELIDGQSVDFGSTNSGQPVTRVITVTNVGGSALALTPL
                     NPASLPAGFSLIANLGNTSLAAGQATQFSVGLDAAAFGTFGGGLQLLNNDSDESPFDL
                     VLSGTVLAPEVTIRLGGENLIDGQTIEFGVTPVGDAVTRTLTVTNDGNDTLTLTPLDP
                     AGLPPGFTLVSNVGGTSLAPGQSTSFSLQFDAASAGSFGGNVALSSSDSDENPFDLVL
                     SASAYVPAPEITALVAGQPLALGGLVDFGATVPGVTLERTITIRNDGTAALNLTPLDP
                     ASLPAGFSLYQDLSSTVLEPGQTTSFILRLDAAAVGEFGGTVTLGNSDSDEGPFTFAV
                     GGSVAPPPPPFKAIVDDGNAGSTLSGAWSVVSGKGYGNDIRTANRGTGTTAATWTFNG
                     LATGQYQAWATWKIASTNATNAPFTIFDNTTSRGTTLVNQQLTPSGLSADGALWKSLG
                     VVTINSGRMVVRLTNAANNKVVADAIRIERVGVQGPAPEIGVSVGGYQMTSGQSSISF
                     GTREIGSPLTYSFVVSNHGAGSLQLTALDPLALPAGFSLVQNFGSTFLTPGESTTFSL
                     RLDAGAAGSCGGALVLANNDADESLFSFNVSGSVVDPNAPFVQIIDEGAAGNTLTGTW
                     TAISGKGYANDMRTAAAGSGSIQSNWTFSGLADGQYQVWATWRIGSTYATNAPFRLYN
                     GNQLVATTAVNQRLTPTGLWDGTTLWQSLGAVSVVGSQLGVRLTNAANGQVVADAIRI
                     ERVPSGAAPITSQKRLGGESLAAWLSASLATASSHQIAEQREPQAALPALTAWKSPAD
                     LWARPKRTSPEESLLASTLELLSSSRSTASAELATALQAAADQLALAALDA"
BASE COUNT         1930 a         3899 c         3945 g         2216 t
ORIGIN      
        1 tgaagcggct gctgctctcg agcacgagaa tcaccgacgc aggcctcgtg cacctctccg
       61 gcttgtcgaa tctcgtggag ctggccctgg ccgataaccc gatctccgac gccggtctgg
      121 cccggctgcg cggcctggtg aaactcaccg agctggacct ggaacgcacc gccgttaccg
      181 atctcggcct gccggcgctg gcggaaatgc gccggctgga aacgctgaat gtacggcgca
      241 cgaaggtcac gacgccagga ctgcggcagt tggcggggct caccgagctg cggcggctgt
      301 gggcgggcga atcactgctg cggtccgcgc cgcccgccag gcaatggcaa gtctggctgg
      361 ccagtgccta cggcgacgac atttacatct acgaactagg ttcctttcag ctgctgaagc
      421 ggattcaggt cgggcccaat ccgcacggct tgtcggcgac cgcggacggg cggacggtac
      481 atgtcgcgct cgagcatttc gacgacccgg tcggagaatt ggcctggatc gacacgcggt
      541 cgtttgacgt caccgcgaaa gtcaaggtcg gcccgcagcc gcaggaaatt gactgcacgc
      601 cggacggaaa gtggatttac gtcccatgcg ccgacgggca gtggcacgtg gtcgatggcc
      661 acgagcggcg cgtcgtcacg accattcaca cggggggccg gccccacaat accgttgcat
      721 cgccggacgg caagcggatg tatctctcgc ccatgggcta tccccaggcc gtgaccgtgg
      781 tcgatgtcgc cgcgggtcac cgcgtcatcg gcgaaatccc gttccaggac gtgacgcggc
      841 cgccggccat caccgccgac gagcggctgt tcttccagaa catcgacaac atgctgggat
      901 ttcaggcggc cgacattgcc caacgccgag ttatcaagac gatcgagcac tcgattcccc
      961 cgcagcggcg cggcgagtgg agccgcagcc acggactggg cgtgcgtccc gaccagcagg
     1021 agatctggtc gtgcaacgtc gagcatcacc tggtccacgt gcacgagatg agcagcggga
     1081 actaccgcga aatcgccagc attacgctgc ccgggccggt ctattggctt tcgttttcgc
     1141 cggacagcaa gtactgcttc gtaagcgtca gaagcaagcg ccaagtggcg gtcatcgact
     1201 gcatttccaa gaacatcgtg acgatgctcg atgcgggcca ggcggtgaaa cgcacgcagg
     1261 tcgtcggcgt gccgctcgac tgattcccgc cgccgctact tcgcgctggc ccggcaatac
     1321 tgcagcgaca tcagggcgaa ggccgtgccg tactgctggt ggtagttgta gagcgggtag
     1381 tcccaccacg agccgtcctt ctcctgcagc ggcagcaggc gatgcgccag ctggccccgc
     1441 atttccgcgc ggtcgctttc gggcagctct tcaatgcact tggccgcgta gtagtgtccg
     1501 taatagaaaa agtagccggc gatggcaaac caggattcgt gagggatcgg gcgcttgcgg
     1561 ccgatgtcga gccacatccc gcgggcgaac agccggtcga gccaggcccg cacgacggcg
     1621 tcggtgatgc gcttgtcgcc atagagccgc agcgtgtagt tgcaggcctg cgagcgaccg
     1681 gagctgccgc cgggcaggtt caccggatgc tgcggcatga gcagcaggta gtcgtcgtag
     1741 acgtagctgc cgtcgggctt ccgctggcgg cgaatcgtcg ccaggccgcg gtcgatcagc
     1801 ttctgcggga cctgaatgcc gatttcggtc gcttcgtgca gcgccaccag cacggtggcg
     1861 gtcgtgaagc tggtcggttc gccagagggc ttctttgtct gggcgttgaa atcgtagtag
     1921 ccccagccgc cgttggccgt ctcgtagcgg cccaacaatt cgatttgctg ctcgatctgc
     1981 ttcttgattt cggcttgctt gtccgtgtag ccgctacggt gcttgtaaag ccgcacgagg
     2041 ccggagatgc cataggcgtg gccccagacg ttgtagatcg ccatcggctc ggcgcgccgc
     2101 aagtgcggca ggttttcgag cagccatttc tcgccgccgt cgatggccgt actcacctcc
     2161 ggccgccggt cttcgacttc cagcaaggcc gagacgcaca tcgcggtcac cgccgagcga
     2221 aaggcgtgat gcgcgccggg aaccggcgcg taaatgttga ggtccttcgt ccgctcggct
     2281 gagccccagg atccgtcctt gttctggtcg gccaggagaa aatcgacgcc gcggcggatc
     2341 gcctcgtcga gcgcctggcg ggtgggtgct tcgaccggct taggccgcgg tgcgacttca
     2401 tccagcggct ttggcttggt cgcctcgtcg tcggccagcg ccagaccgcc cggcacaagc
     2461 agcagaagca gtccgagcgc gcgaatgcag ttcatggctg gctccagtta tcccgaggcc
     2521 agcgtgatta taactacgcc gctgccggag gacggcccct acagcattat tgcgtcggcg
     2581 ggctgatcag cgggccagag ccgccgaggc cgcccttcag ctccccgcgg ggcttctttt
     2641 ccttgggagg agcggcaggc gtgccgtcgg cgttgatgtt tggagcgccg gaaaggtcgt
     2701 cctcggccca ttcgacgcgt ttccgcgaga ggccgatctt gcggtcgtcg gtatcgacgc
     2761 ggaggatctt cacgtcgatc ttctcgccga ccttcaccac gtcctccggg ttctcgacct
     2821 tgtggtcggc cagctccgag atgtggagca gcccctccag gccgtcctcc agcccgacga
     2881 agacgccgaa gttggtgatc ttcgtcacgg tgccgctcac gatttgcccc ggctgatact
     2941 tggccgggat gttcgtattc cacggatcgt cgtcgagctg cttcatgccc agggcgatcc
     3001 gccgccgctc ttgatcgacg gagagaatct tgcaccgcac ggcctgcccc ttctcgagca
     3061 tctcgttggg gtggctgatc ttgcgggtcc aggacatgtc gctgacgtgg agcaggccgt
     3121 cgatgccttc ctccagctcg acgaacgcgc cgtagttcgt caggttgcgg accttgccct
     3181 cgacgatctt atcgaccgga tacttttcgg cgacgcgagt ccacgggttc tcctgtgtct
     3241 gcttcatgcc cagcgagagc tgctgcccgg ccttatcgac gccgagaatc accacgctga
     3301 tctcgtcgcc gatattgacc agctcgctgg gatggttgac gcggcgggtc caggacatct
     3361 cgctgatgtg caccaggccc tcgatgcccg gctccagctt cacgaaggcc ccgtagctca
     3421 tcacgttgac gaccgagccc ttgtggctgc tgccgacggg gtacttctcc tcgacgttgt
     3481 cccaggggct ggcgcccttc tgcttcaagc ccagggcgat cttctttttc tcgcggtcga
     3541 tgtgcaggac catcacctcg atttcctggt cgatcgccac catctcgctc ggatggccga
     3601 tccgctccca gctcatgtcg gtgatgtgca gcaggccgtc gatgccgccc agatcgacga
     3661 acgcgccgaa atcggcgatg ttcttcacca cgcccttgcg gacctggctg acctccaatt
     3721 ccttgagcag ggcctcgcgg tcttcttcgc gctgccgctc gatcaggctc cgccggctga
     3781 cgacgatgtt gcggcgggcc tcgtcgatct tgagcacttc gcactgcacc gtgcggccga
     3841 tgtagtcgcc gatgtcggag gggcggcgga tatcgacctg gctggcgggc aagaaaacct
     3901 tcacgccgcc gatatcgacg agcaaaccgc ccttgatctt ccgctcgacc acgcccgtga
     3961 ccgggtcccc ttccttcagc ttgctgatca cctcgttcca ctggatcagc cgatcggctc
     4021 cgtttttcgt gatgcggacc attccgccgt cgtcggggcc ggcttgttca tcctccaggt
     4081 cctggatgag caccttcacg agcatgccga cggtcggcgg ctcttcgcct tcgcgccact
     4141 cgtcggccgg caccgagccc tcgctcttga agccgatatc gaccatcacc atgccgtctt
     4201 cgacccgcag aatgcggcct tcgacgatct tgccggcctc gaattcggat ccgccgccgc
     4261 tctcttgaaa cagccccgct tcctcgaaag tcgccgtcgc agcggcgaac atcgactcga
     4321 tttcggggtc gttctccagg tttcgaatga gtgctcgatt gaccattgct ttcgttgctc
     4381 ttgcgatcta ctgcgccggt tcaggccgca gcaggctgcc aggatcgccc gctgccaagc
     4441 cccgcgcacc aaaggcctac gctttcgcct cgagcctcca caggaaggcc gaaaatgggc
     4501 caaagcgaat cccgaagtat atagcggtca agcacttccg ccaagcgtac gatgccgcta
     4561 ggctgctcga aacagcggag gttggccttc gtcatgggaa tcgcggcatt cactgggaag
     4621 ctggcctgcg gactgctcct cgcggcagca ttggccagca gcggaccagg ccaggagcgc
     4681 cagccgctgc cgacgccgcg gcaaatggcc gactcgcgcg aggatctttg ggccgaggcg
     4741 gcgatcaagc aaccgggcgg accgagttac gaattcttcg caccgctcct gccgccgctg
     4801 cgctatgtca acacggcgtt ccggcattac cccatcgtgc tcagtgcgcc cggcgcgccc
     4861 gtcaaggcgc ggtgggtgag cgatggcagc ggcgtgaacc tgcgggcgga caagccgccg
     4921 atgtggcgcg agatcggcac gcccgtggag ttcttcgtag gggcggacag gaagcctttt
     4981 ggcgcggatc ccgcacagct cagcgagccg gcgctcgagg acggctatct gccgatcttg
     5041 cagcttcaat atgagcatgc cggcacgaag tacgaccaac aggccctcgc cgtcacgcgc
     5101 ggagcagcca gcgagtacgg cgcggtcctc gtccgttttc gctgcccggg caaggcaggc
     5161 atcgtctcgg cccgtatcgg cggcgacgac cggctgacgg tcaaggattc gcggatcgag
     5221 aacgccgcag gtcaatcgct gacctcattt ggcccgccgt ggcgctggga ttccgatcgg
     5281 cgctcgctcg tcgcttcgct cgcagccgaa gcggccgcga cgctgctgat cgctacgcag
     5341 cccagcccgg agcctctgcc ggacaaatcg tggaacgcag cacacgcaga ggcagtcgcc
     5401 gattggcggg cgatcctggc gcgcgggacg cagctgacga ttcccgaacc gctggtgcag
     5461 gatgcctggc gggcgctggt tctgggcaac tatgcgatcg ccagcggcga ccaaatgaac
     5521 tacagcgcgg gcaacgccta cgaccatctc tacgaaggag agtgcggcga tgccgcgagg
     5581 tcgctgatgc tcttcgggca gcaatccgac gcgcggcgga tgatcgggcc cctgctcgat
     5641 tttcagcgtc aggccacgcg attccacgtg gcggggcaca agctgcagct gctgacgcac
     5701 tactactggg tgactcgtga cgcggaatac ctgcgggaaa agcgcggcgt gtgggagcca
     5761 gtggccagtt tcattcgcac cagccgacag cccgagcacg gcctgctgcc gaaggacaac
     5821 tacgccggcg acatcccgca acaggtctgg tcgctgagct ccaattcgaa ttgctggcgc
     5881 gggctgcggg acctgggggc cgtactggaa gactgcggcg aaaagtccct ggcggcggag
     5941 ctgcgccggg aagccaccga gtatcgtgcc gcgatcctgg ccgccgtcga gaagagcatc
     6001 aaccgcagca cgcagccgcc gttcattccc atcgccttgc tcggcgacga gccggcgcac
     6061 gatccgctca cggcgactcg catgggcagt tattacgacc tgatgacgcc gtacgtgctt
     6121 ggttccggcg ttttccgtcc cggcgaccag cgggagcaat ggttgatcga ctactttcgc
     6181 cggcacggcg gcctggccat gggcatgatt cgcagccagc ctcaccaggg cgaattccag
     6241 ggccagcccg gcgtgaacgt actctacggg caaaggtaca tgctgacgct cttgcggcgc
     6301 ggcgaccgcg aacacgccct ggccggattc tatggccacc tggcccaggg gatgacgcgg
     6361 ggcacgttca tcggcggcga aggctcgcgg tttttccacg gcgatgaacg aggccgctcg
     6421 ttctatctgc cgcccaacag cgcctcgaat gcgatgttcc tgaccacgct ccgttacctg
     6481 ctcgtgcagg attgggacct ggacgacgac ggcgagcccg aaacgctgcg gctattcgac
     6541 gcgattccgc cgcgctggct cgccgacggc cagacgctcg ccgcccagcg gctgccgacg
     6601 gcgtttggcg agctgtcgtt tcgcgccgtg tcgcagtttg ccgatcggcg tctccatgtc
     6661 actctggagc tgccgccacg gccggcggag cgtgtcacgc tccggctccc caaccctcca
     6721 ggccacaaga ttgaggcggc ccaaatgggc gggcggacga tcgcgcctga cgcagaggga
     6781 cgcttcgatc tgcgaggcgc ggcgggccgc tctgagctgg tggtaacggt gcgcaaggat
     6841 tagcggccgc ggtgtgcgcc aattgccgcg agaaatcttg gcgattccct acttccgagc
     6901 cggggctagt caagaaagtt tgccagggca agcaaaatca agcggaagtc atgtattacc
     6961 agtagtgacc ggcggagcgt cttcgtcatg ccgcatcgct gccaggaaac tcttgccgcc
     7021 tgcccgcctt gaagctccta cagttgctcg tgctgatcct gctgggcggg gttttctggt
     7081 gcggctgcca gtggcccgcc acaccgcagt gggtgagttc atcgcgcacg atgccctggg
     7141 cccgcatcca gaactatgct gaatcgtcct ccgcacctcg atccgccaca gccagcagcg
     7201 ggcgcttacc cgccaaacca caaatgccgg cttggattta tctcgccttc atcgtaggct
     7261 cttcggtctt cttgagcctg gtcgatcgct ggcgggtgga gatcgcgcgc cgttttgctc
     7321 gcgagaatca cctcgccctg ccgcggatcg tcacgcagcc gctcgtgctg cgccgccatc
     7381 tgctgttggc attggccggc tgcatcgcct tggccgtgta tggctcgctg gtgccgttcc
     7441 acgccagacc gcagacgtgg ccagcggcgc tgcgtgaatt cggcgatggt ctggtccgcc
     7501 cgctcagctt cggctcgaag acggattggg cgaccaacgt cctgctattc gtgcccatcg
     7561 gctttctggc gaccggagcg gcgctcggtc gagtcgcgag cgatcgccgc cgggcgctgc
     7621 taccgccggc gatcgtggct ggctgcacgc tcttgagcct ggcggttgag tttggccagt
     7681 tgtgggtggc cgatcgattc tgttcgcaaa atgacatcgt ggcggaaagc ctgggcggcc
     7741 tcgtgggcgt cagcctgtgg ctggtcagcg gctcgacggt ggcaacctgg ctcgatgcct
     7801 ttcggaccca gcgccgaccg cgcgagcggc tggagcgatt gctccaggtg tatctcgggg
     7861 cggtcttggt gtgggtggtg ctgccgctgg acttgacgct gcggccggcg gagatctggg
     7921 acaagtttca ggcggggcgc atcaatctga tccctttctc cgacctgcag tggacgaccg
     7981 ccggcgccgt cggctggctc gaaaaactgt tgctcctcct gccgctcggc gcgctcgcgg
     8041 ccctctggcg ctggccgcgc gcggatagtg tccgcccgct gggccgggcg ctgcttctgg
     8101 gcgcagggct gatcgcctcg ctggaggctt gccagctgct ggtcttcagc cgcacctgct
     8161 ccaccaccga cgtcgtatcg ggattggccg caatcgctgc cggctgggcg ctggccaggt
     8221 ggcggctgca tccggcggca attgattccg gtccgcggag cggatggctg gcactggctg
     8281 tcgtcgccgt cggattctgg gcggcgctgg cggcggtctt ttgcctgcct ttcgatcgcg
     8341 tcactagcag cgcagaagcc gcccagcgct ggcaggcgct gtggtcaacg ccaatgctgg
     8401 ccacgtttca atcgggcgct atctcgaacg ccctggcgga agccttgcga aagacgctgc
     8461 tgtttggaac ctcgggggct ctcgtgggct tgctggcgcg gccgtttgcg cccggctcgc
     8521 cgcaacggac ccccgccatt ctgtgcgcga tgggcctgca aatcgccttg cctttggcga
     8581 tcgaattcgc tcaactttgg ttgcctcctc accagccgga actgggcgac gtcctgctgg
     8641 gaacgatcgg cggcgggctg ggaatggtgg gagtcttgac cgcggaccgc gacctctcga
     8701 tcgctgcccc ggacctcaag gatggaaggc ggtgaacggt cccccaagat ggcggccgtt
     8761 gcgggttccc gcaatttgcg attctttaga agccgcggtc gggaaaaaaa ctttggccga
     8821 gatagtgcgg tttcgcgcaa tttgcgtcag taagtcgccg atagcggtct agagcatgta
     8881 gaaagcaggt gcgacacatt aacgctgggt gaaatcttga acgcgtcaaa gttgttttgg
     8941 cctctcccca actccatgac gaaaagccct cgacgatcgc ggtcgaagtc gcctgcgggc
     9001 ggtttggctg gcatcacgct gtccgaattg cacgcccgcc gcgtggccat tcatctggtc
     9061 gcgctaggcc gcgagcaatt cattcggggg caggggcagt tcgaggtcga tccggacctg
     9121 ggacgcgtgc tgcgagtgaa ggtcaaggat ccggacggcg attttgagct cgtcgtttcg
     9181 gaggcgaagt ggaacggcca ggtgcttccc ggccaggcgg ttggatgcga ctacctgttg
     9241 cggctcggct aagggcccgc gcgcttacgc atcgagcgct gccagcgcga gttgatcggc
     9301 ggctgcttgc agcgctgtcg ccagctcggc gctcgccgtg ctgcgcgagg agctcagcag
     9361 ttccagcgtg ctcgccagca gcgactcctc cggggaagtc cgcttgggcc gcgcccacag
     9421 atcggcggga gatttccacg ctgtcaatgc gggcagtgct gcttgcggct cgcgctgttc
     9481 ggcgatctgg tgagaggagg ctgtggccag cgacgccgac agccaggcag ccaggctttc
     9541 gccgcccagc cgcttctggc tggtgatcgg tgcagcgccg ctgggtacgc gctcgatccg
     9601 aatcgcatcg gccacgacct ggccgttggc ggcgttggtc agacgaacgc ccagctggct
     9661 gccgacaaca ctaaccgcgc ccagcgactg ccacagcgtg gtgccatccc acagtccggt
     9721 gggcgtcagc cgctgattga cggccgtcgt ggcaacgagt tggttgccgt tataaagccg
     9781 gaagggcgcg ttggtcgcat acgtgctgcc gatcctccag gtggcccaaa cctggtactg
     9841 cccatcggcc aggccgctga acgtccagtt cgattggatg ctccccgaac cggccgccgc
     9901 ggtgcgcata tcgttggcat agcctttccc gctgatggcc gtccaggtgc cggtcaaggt
     9961 gttgccggcc gcgccctcat cgatgatctg cacgaagggg gcgttgggat cgacgaccga
    10021 cccgctcacg ttgaacgaga acaggctctc gtcggcatcg ttattggcca gcacgagcgc
    10081 gccgccgcac gagcccgcgg cccccgcgtc cagccgcagc gaaaaggtcg tcgattcgcc
    10141 gggcgtgagg aaagtcgagc cgaagttttg cacgagcgaa aaacccgcgg gcagcgccag
    10201 cggatcgagc gcggtgagct gcaagctgcc ggcgccgtgg ttcgaaacca cgaacgaata
    10261 agtcagcggg ctgccgatct cgcgggtccc aaagctgatg ctcgactggc ccgacgtcat
    10321 ttggtatccg ccgacggaca cgccgatttc cggtgcgggg ccttggactc ctacgcgctc
    10381 gatgcggatc gcgtcggcca ccaccttgtt attggcggcg ttggtcagcc ggacgaccat
    10441 gcggccgcta ttgatcgtca cgacgccaag cgacttccac agcgcgccgt cggcgctgag
    10501 cccgctgggc gtcagctgtt gattgaccag cgtcgtgccg cggctagtcg tgttatcgaa
    10561 gatggtgaag ggggcgttgg tggcgttggt cgaggcgatc ttccacgtcg cccaggcctg
    10621 gtattgtccc gtcgccagac cattgaaggt ccaggtcgcc gccgtcgtgc cggtcccgcg
    10681 gttggccgtg cggatatcgt ttccatagcc cttgccgctg accacgctcc aggcccccga
    10741 cagcgtgctg ccggcattgc cgtcgtccac gatcgccttg aatggcggag gaggaggagc
    10801 cacgcttccg ccgaccgcga aggtaaacgg gccctcgtcg gagtcggagt tgccgagcgt
    10861 caccgtgccg ccaaattcgc ccaccgccgc ggcatccagg cggaggatga acgaagtcgt
    10921 ctggcccggc tcgagcaccg tgcttgacag gtcttggtag aggctgaagc cggcgggcag
    10981 ggacgcggga tccagcggcg tcaggttgag cgccgccgtg ccgtcgttgc gaatcgtgat
    11041 cgtgcgctcc agcgtcacgc ccgggaccgt cgcaccgaaa tcgacgagcc cacccagggc
    11101 cagcggctgt cccgcgacga gcgccgtgat ttcgggcgca ggcacatagg ccgaggcgct
    11161 gaggacgagg tcgaacgggt tctcatcgga gtcgctgctg ctcaaggcca cattgccgcc
    11221 gaacgagccg gccgaggcgg cgtcgaactg gagcgaaaag ctcgtcgatt gtcccggagc
    11281 gagcgatgtt cccccgacgt tcgagacgag cgtgaacccc gggggcagtc cggctggatc
    11341 gagcggcgtc agcgtcagcg tgtcgttgcc gtcgttggtg acagtaaggg tccgcgtgac
    11401 ggcgtcgccg acgggcgtga cgccgaattc gatggtctgg ccgtcgatga ggttttcgcc
    11461 gcccagtcgg atggtgacct ccggcgcaag aaccgtgccg gacagaacca ggtcgaaggg
    11521 actctcgtcc gagtcgttgt tcagcagttg cagcccgccg ccaaacgtac cgaaggccgc
    11581 cgcatccagg ccgaccgaaa actgcgtcgc ttgcccggcg gcgagcgacg tgttgcccag
    11641 gttcgcgatc agcgaaaagc cggccggcag actggccgga ttcagcggcg taagcgccag
    11701 agcggagccg cccacgtttg taaccgtaat cacgcgcgtg acgggctgcc cgctattggt
    11761 cgaaccgaaa tcgacactct ggccgtcaat caattcagcc ccgccggcgg tcacttcgat
    11821 ctccgggggc ggtcccaccg gcactgagcc ggtgatggtg tactggccca tgtcgccata
    11881 gtccccccgg ctgcgggcga ccaggtaata cgtgcccgcg gcgaccgtcg tagaaaaact
    11941 cgcccccagc gagttgctcg gcgaactggt gagcagcacg ctgctgccgt
//
LOCUS       DASRJB010000423         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3218566, whole genome shotgun sequence.
ACCESSION   DASRJB010000423 DASRJB010000000
VERSION     DASRJB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218566"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFB80_11190"
     CDS             complement(<1..782)
                     /locus_tag="VFB80_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34379.1"
                     /translation="MKRLLFCALGLVAAALTFTSRAQAQTNDVQYASTTNSELSDLYA
                     RLANLEARAATNNGGGGGCGCNDCCCGRSGFVAAGEIMWLKAYNSDNDFGEFNFREGY
                     RVWFGWQGDSGLGARLRYFDYFQRAANTGDTLSIYAFDAEIYDNVELGCYWDLLVGAG
                     FRVFGYEQNLDGGGAPLDALWGVGPVITAELYRHLGDRAALYAIGRQSIIVGSGRDDG
                     GVQQDDTGSVTELQLGLQLHTYWGDSLVFGRVGWETQAYYDI"
     gene            complement(999..>2460)
                     /gene="tatC"
                     /locus_tag="VFB80_11195"
     CDS             complement(999..>2460)
                     /gene="tatC"
                     /locus_tag="VFB80_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZN34380.1"
                     /translation="KQPHDDLFEKTTMSFGDHLEELRVCLFRGVVGIGLGCVIGFFIA
                     NRVVRFFQSPLEEAMQRYYLDKTLTDYQAEFGEPPLEVQKMVLEEGFVFEKFQVESAA
                     IADALRLTYPQRLGELDMSRYWFTAGDFQPRSGLKPGGALILCRQLDAAKDDSKSPAG
                     SLWQLLTAEQRKRVETLAAAGQELDAKQTAEILQILNSLAAQPALHDSPQWAAISGPD
                     QTAAENLSTWVLSLLGRKPVATDTVSTIRQQLAAKPDAEGTRRLNKLLLARVFPEALR
                     AARLNLLTIYSWKPVKVRFQVLNAQEAFMIWMKAGLMSGLILASPYVIYQIWIFVAAG
                     LYPHEKHYVYLYLPISFALFLGGASLAFAFVFRPVLDFLFTFNKGMNADFDPRIGEWL
                     SFVLILPLGFGISFQLPLVMLFLNRIGVVSLDLYLAQWRIAILIICVLSMVLTPADPI
                     SMILMAVPLCLLYLLGIVFCKFMPKGRNPFAEAYEP"
BASE COUNT          451 a          771 c          823 g          415 t
ORIGIN      
        1 tggatgtcgt aataggcctg ggtttcccag ccgacgcggc cgaagacgag cgagtcgccc
       61 caataggtat gcagctggag gccgagctgc agctcggtca cgctgcccgt gtcgtcttgc
      121 tgaacaccgc cgtcatcccg gccgctgccg acgatgatcg actggcggcc gatggcgtac
      181 agggcggcgc gatcgcccag gtggcgatac agctcggccg tgatcaccgg gccgacgccc
      241 cacagggcgt cgagcggggc gcctcccccg tcgaggttct gttcataacc aaacacgcgg
      301 aagccggcgc cgacgagcag gtcccagtag cagcccagct cgacgttgtc gtagatttcc
      361 gcgtcgaagg cgtagatgct gagggtatcg cccgtattcg cggcccgctg gaagtagtcg
      421 aagtaccgca gccgggcgcc caggccgctg tcgccctgcc agccgaacca gacgcgatag
      481 ccttcgcgga aattgaattc gccgaagtcg ttgtccgaat tatacgcctt cagccacatg
      541 atctcgcccg cggcgacaaa gccggagcgc ccgcagcagc aatcgttgca gccgcacccg
      601 ccgccgccgc cgttgttggt ggccgcgcgg gcttcgaggt tcgcgaggcg agcgtagagg
      661 tcgctcagct cgctgttggt tgtactggca tactgcacgt cgttcgtctg ggcctgggcc
      721 cgcgacgtga atgtcagcgc cgcggccacc aggcccagcg cgcaaaagag gagccgcttc
      781 atatccgcct cgtccgtgaa tgcctggaag atttgacgga gtccatttcc gcctgcagtt
      841 ttttcatcgg cggctcgcgg cggccggaat gaccgcttat gacgcccatg cccagcgaat
      901 tacccccccg cgcgtgaagc tgtgcgcgtg gtacgggata tccggcatat ccggccgccg
      961 gccgccgcta gcggcccttg ggaaaacgcc gcaagtgcct acggctcata ggcttcggcg
     1021 aagggattgc ggcccttggg catgaacttg cagaacacga ttcccagcaa atagaggagg
     1081 cacaggggca cggccatcag gatcatgctg atcgggtcgg ccggcgtgag gaccatcgaa
     1141 aggacacaga tgatcaggat cgcgatccgc cactgggcca ggtacaggtc gagcgagacg
     1201 acgccgatcc ggttcaggaa cagcatcacc agcggcagct gaaagctgat gccgaagccc
     1261 agcggcagga tcagcacgaa cgagagccac tcgccgatcc gcggatcgaa gtcggcgttc
     1321 atgcccttgt tgaacgtgaa caggaagtcg agcaccgggc ggaacacgaa ggcgaacgcc
     1381 agcgaggccc cgcccaggaa cagggcgaag ctgatcggca ggtagagata gacgtagtgc
     1441 ttctcgtgcg gatagaggcc cgccgcaacg aagatccaga tttgatagat cacgtagggg
     1501 ctggccagga tcaggccgct catcaggccg gccttcatcc agatcatgaa cgcctcctgg
     1561 gcgttgagca cctggaagcg gaccttcacc ggcttccagg aatagatggt cagcaggttc
     1621 agccgggcgg cgcggagggc ttccggaaag acgcgggcca ggagcagctt gttgagtcgc
     1681 cgcgtgccct cggcatccgg tttggcggcc agctgctggc gaatcgtgct gaccgtgtcg
     1741 gtcgcgaccg gcttgcggcc gaggagcgac agaacccacg tggagaggtt ttcggccgcc
     1801 gtctggtccg gcccggagat cgcggcccac tgcggcgagt cgtgcagcgc cggctgagcg
     1861 gcgagggagt tgaggatttg cagtatttcc gcggtttgtt tggcgtcgag ctcctgtccg
     1921 gctgcggcca gcgtttccac gcgcttccgc tgctcggcgg tgagcagctg ccacaggctg
     1981 ccggcgggcg atttcgagtc gtccttggcg gcgtccagct ggcgacagag gatcagcgcg
     2041 ccgccgggct tcagtccgct tctgggctgg aagtcgccgg ccgtgaacca gtagcgcgac
     2101 atatcgagct cgcccagccg ctgcggatag gtcagccgca gggcgtcggc gatcgcggcc
     2161 gattcgacct ggaacttctc gaaaacgaac ccttcttcga ggaccatctt ctggacctcg
     2221 agaggcggct cgccgaactc ggcctggtag tcggtcagcg tcttgtcgag gtaataccgc
     2281 tgcatcgctt cttccagcgg gctctgaaag aagcggacca cgcggttggc gatgaagaac
     2341 ccgatcacgc agcccaggcc gatgcccacc acgccgcgga acaggcacac ccggagctcc
     2401 tccaggtggt cgccgaaaga catcgtggtc ttttcaaaca ggtcgtcgtg gggttgcttg
//
LOCUS       DASRJB010000424         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2374892, whole genome shotgun sequence.
ACCESSION   DASRJB010000424 DASRJB010000000
VERSION     DASRJB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374892"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..1784
                     /locus_tag="VFB80_11200"
     CDS             468..1784
                     /locus_tag="VFB80_11200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN34381.1"
                     /translation="MLSFILVTAIFNLALGYLAAAALADPPAWTHWRLPASWRRRAST
                     KRSTGDGGASRAAAGTERGHTLPSSPPRTIAGLEELPRQWLDQLVRAGIVAQSFLEGA
                     AQVLRLEVGFYREQLVGVETRCRALLASSDSAGLKLLADDLRAANQDWLTKQTAAATM
                     FAQRSGRLGDHEASAQALEQTLLDQAAQICGVNDQLEALTFPGETDSGGKQLLEHLAT
                     LVAAAHALRDQMLDLVATLLRGGQRLGELREAALLDQGTRLPNRLGVESILAAWFQGD
                     PQHSRQLCLVLIDLDRFGRVTQRLGTRAGDRALVAASGLIDELIRKDREHDRICRFGG
                     QSFLVVMGDAGPHQALAAAERLRQSFEATTFDDHGATFELTLSCGVVEVARTESVTEL
                     LRRLVLAVQFAKKAGRNRCALDKGQGPTTLDPPQFPVKGRVVSLGE"
     gene            1915..>2870
                     /locus_tag="VFB80_11205"
     CDS             1915..>2870
                     /locus_tag="VFB80_11205"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34382.1"
                     /translation="MLTILGRGQKYCDGISRRSFLRIGGLGLGAGALSLASLHRLEAA
                     SGQRSSKAVINIFLGGGPPHQDLFDLKLEAPAEIRGEFSPIETKVPGIQICQVLPQLA
                     RIMDRCAIIRSVVGATDRHDAFQCMTGWLVKDLASLGGRPSLGSVTAKLLGPTDPAVP
                     PFVGLAKPTKEIRWSDAGKPGFLGPAFGPFKPDGEGLDNMVLRGLTLEQLQDRRKLLG
                     SLDRLQRDAHAGGQIQAVDRFTAGAFDVLTSSRLAEALDLSKEDPKIRERYGTGQPYN
                     YQFDGAPTVNEHLLLARRLVEAGVRVVTLSYGRWDSHSKNFDL"
BASE COUNT          477 a          937 c          987 g          469 t
ORIGIN      
        1 tcgttgccat aaccccacag gcccgcccac gaatttggaa tgcgtccgcc ggtggccgca
       61 aagtagccgg ccacagcatc gtagccaggc ggagtcccgt agggtgaata gtaatagcct
      121 gccgaaccag ccggctgagc cccgtgaggt gcggcgggca cgggttcgac ggaaggctga
      181 ggagcagctg gcagagcctc ggcgggacgt gtcgcgggcg gaggctgctc ctcctgagcg
      241 cgcaggggtg cagcaaccag tgtcaggatg agcaaagtgg tccaaatccg ctgcatctgg
      301 cgctccccgc tgctcgccgc ttataccgat tcttcggctt gtatcgaccg tgcggcctgt
      361 aacagaaaag cagaatagag ggggttcggg tcgggcaagc tgggcaagcc acgtcgagcc
      421 ttgaatgacg tagccttggt aactcaggca aacgagagag ctagcgagtg ctgtccttca
      481 ttctcgtaac agcgattttc aacctggcgc tgggctatct cgcagccgcg gcgctggccg
      541 atccgccagc gtggacccac tggcggctgc cggcaagttg gcgacgtcgg gctagcacga
      601 aacgctccac cggggacgga ggggcaagcc gagcggcagc cggcacggag cgggggcata
      661 ccctgccctc atcgccgcct cgcacaatcg ccgggctgga agagttgccg cgtcagtggc
      721 tcgatcaact cgtgcgggcg ggcatcgtcg cgcagtcgtt cctggaaggc gccgcgcaag
      781 tgctgcggct ggaagttggc ttctatcgcg agcagctggt cggcgtcgaa actcgctgcc
      841 gcgcgctgct ggcttcgtcc gacagcgccg ggctgaaatt gctggccgac gacttgcggg
      901 ccgccaacca ggactggctc acgaagcaga cggcggccgc gaccatgttc gcccagcgca
      961 gcggacgact gggggaccac gaagcctcgg ctcaagccct ggagcagacg ctgctcgacc
     1021 aggcggcgca gatttgcggc gtcaacgacc agctcgaggc attgaccttt cccggcgaaa
     1081 ccgactccgg cggcaagcag ctcttggagc atctggcgac gctggtcgcg gccgctcacg
     1141 cgctccgcga ccagatgctc gacctggtgg ccacgctgct ccgcggcggc cagcgcctgg
     1201 gtgagctgcg cgaggcggcg ctgctcgacc agggaacgcg gctgcccaac cggctaggcg
     1261 tcgagtcgat tctggccgcg tggttccagg gtgatccgca gcactcgcgg cagctgtgcc
     1321 tggtgctgat cgacctggac cgcttcggcc gcgtgacgca gcggctgggc acgcgggccg
     1381 gggaccgagc actggttgcc gcctcggggc tgatcgacga gctgatccgc aaggaccgcg
     1441 agcacgaccg catctgccgg ttcggcgggc aatcgttttt ggtcgttatg ggcgacgcgg
     1501 gaccgcatca agcgctggct gccgccgaac gcctgcggca atcgttcgag gcgacgacgt
     1561 tcgacgacca cggggcgacc ttcgagttga cgctgagctg cggcgtcgtc gaggttgccc
     1621 gcactgagtc ggttaccgag ctgcttcggc gtctggttct ggcggtgcaa ttcgccaaga
     1681 aggccggccg caatcgctgc gcgctcgaca aggggcaggg accgacgacg ctcgacccgc
     1741 cgcagtttcc ggtcaaagga cgcgtggtca gtctcggcga atagtcgcgt cggcgagtga
     1801 ccgacctgcc tcgccatgcc gccgatctct ttattgacgg ccggcggcga cggtcctaaa
     1861 ttgcaagttg ggattgctgt ttgcgcacgc cccctgaagt gcaggattcc gcccatgctc
     1921 accatcctgg gacgcggcca gaaatactgt gacggcattt cgcgccggag ctttctgcga
     1981 atcggcgggc tggggctcgg cgcaggagcg ctctcgctgg ccagcctgca ccggctcgaa
     2041 gcggccagcg gccagcgctc ctcgaaggcg gtgatcaaca tcttcctcgg cggcggaccg
     2101 ccgcatcagg acttgtttga tctgaagctc gaggcccctg cggagatccg cggcgagttt
     2161 tcgcccatcg aaaccaaggt gccgggcatc cagatttgcc aggttctgcc gcagctggcc
     2221 cgcatcatgg accgctgcgc gatcattcgc tcggtcgtcg gggcgaccga ccgccacgac
     2281 gctttccagt gcatgacggg ctggctcgtc aaagatttgg cctcgctcgg cggccgtccc
     2341 agcctgggtt cggtcacggc gaagctgctc gggcccaccg atcccgcggt gccgccgttc
     2401 gtcggcctcg cgaaacccac gaaggaaatc cgctggtcgg atgcgggcaa gcccggcttt
     2461 ttgggaccgg cgttcgggcc gttcaagccc gacggcgaag gcctcgacaa tatggtcctg
     2521 cgcgggctca cgctggagca gctgcaggac cgccgcaagc tgctgggcag cctcgaccgg
     2581 ctgcagcgcg acgcccacgc cggcggccag attcaggccg tcgatcgatt cacggccggc
     2641 gcgtttgacg tgctcaccag cagccgcctg gccgaagcgc tcgacctgtc gaaagaggac
     2701 cccaagatcc gggagcgata cggcacgggg cagccctaca actatcagtt cgacggagcg
     2761 ccgacggtca acgagcacct gctgctcgcg cggcggctgg tcgaagcggg cgtgcgggtc
     2821 gtcacgctgt cgtacggccg ctgggacagc cactcgaaaa actttgacct
//
LOCUS       DASRJB010000425         8161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_682017, whole genome shotgun sequence.
ACCESSION   DASRJB010000425 DASRJB010000000
VERSION     DASRJB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682017"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..918)
                     /locus_tag="VFB80_11210"
     CDS             complement(247..918)
                     /locus_tag="VFB80_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34383.1"
                     /translation="MSKEEVWDDKSREELRDTLRRVILSELRLARRDKEEILEACCEA
                     YIQDLSPEDEWDTFIHFATDELKRAAILLATEKAAWPAVTDCDRLDRVEVALRERGIL
                     LWQVSPCCDTCTGAELGDRVNVISRRYPGFRDCVRGYAFFIDQNMPDMLADSTRLSVY
                     LAYGWFSADDSQMSPDAYAKNALGIAREVCECLRNEGFEVDWDVDVARKIGVSLSWQR
                     RTMLE"
     gene            complement(957..2207)
                     /locus_tag="VFB80_11215"
     CDS             complement(957..2207)
                     /locus_tag="VFB80_11215"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN34384.1"
                     /translation="MKRRTTDVYWVVTIALLPLGLAAAQDQAAIKPFQIAVPDDVLRD
                     LQERLARTRWPDQIEGTGWEYGVPLSEMKPLVEFWRTGYDWRGQERKLNELNQFTTRI
                     DGLDIHFVHVRSKEPGALPLVITHGWPGSFVEFQKIIGPLTDPVAHGGKAEDAFHVVC
                     PSLPGFGFSSKPAERGWSSQRMAEVIAKLMARLGYERYGAQGGDWGSGVTRWLASADG
                     GHCIGGHSNFPSGGQPMDDPMRGVTPQEMERMQQRRRELADQYGYSSIQGSRPLTLGY
                     GLNDSPAGLAAWIIDKFWAWSDHGGKLENSFTKDELLTNVMIYWVTETMPSATRIYFE
                     SQHNLPRPASMSAFQSTGRPAPMGFVLFPKEINVPPRAWVERAMGGSLFHWTEMPKGG
                     HFAALEQPQLLAQDVRAFFAKVRK"
     gene            2250..3194
                     /locus_tag="VFB80_11220"
     CDS             2250..3194
                     /locus_tag="VFB80_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34385.1"
                     /translation="MRCLALFTGGLDSQLAVRLMQRQGIAVRAIYFRSAWSDATAAAT
                     AAAGALDVPLAVADLGEKYVQSLRQPRFGYAFGAAPCLDCRIAMLSEARCQLEQQDAE
                     FVVTGEVVGQRVRSAVRDLEIVAHHAGLEGRLLRPLSARLLPATECERSHCVDRARLF
                     DWQGKSRRPQAQLAAELGLAVVPPRSDCPLLSGPLAERALALVRSGQGLRAWDLELLA
                     IGRHEAVGGGARVIVGRNEHENERLTATAAGADAGNGALVFPVNFLGPTALLIGAATE
                     ANVQRAAALVARYSRNMPASPRLRVETSGVTREIELSS"
     gene            3266..3955
                     /locus_tag="VFB80_11225"
     CDS             3266..3955
                     /locus_tag="VFB80_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34386.1"
                     /translation="MPALACASGWCLLLALAGCDAEPEETSLKEQAEAVRRGESDRIL
                     IETAILADDDLRELTGLENLRELLIDSASSRFTADGIGQLIGLPKLEHLRIRGTGVDD
                     QALQHFAGLESLVILNLPQGTFGDAALVELKRLPKLAQLRFSSPRVTDAGMQTLAELP
                     AIKRLHLIHVPITDVGLAELGRIERLESLYIDGGDFSDFAMEKLFRERPRLHIHLNQQ
                     HHDLDPHKHSH"
     gene            3955..5172
                     /gene="ilvA"
                     /locus_tag="VFB80_11230"
     CDS             3955..5172
                     /gene="ilvA"
                     /locus_tag="VFB80_11230"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZN34387.1"
                     /translation="MAASITLADIRAARERIAGAIYVSPCPESPALSELTGSRVFCKL
                     DSLQRTGSFKERGARNALLLLPDDKKKKGVVAASAGNHALALACHGRELGIPVTVVMP
                     QFAPLIKVATCRRFGANVVLCGENFAEAKAKADELAAAGGLTYVHGFDDPAVIAGQGT
                     AGLEILEQVPGLEAVIVPVGGGGLIAGVSLAIKSLQPQVRVIGVEAEVMPSFTQALAA
                     GRAVVCPARPTLADGLATTRMGDNAFAIAEKHVDRLVTVSEEQIALSILRLLELEKSV
                     VEGAAAAPLAALLSGQLPELKRKTVALLLCGGNIDPLVLGRIIEVGLAADGRLARLIA
                     TISDRPGGLAHLASIIAQAGASVQQITHERVFSGPDVSSVRVICDVETRDRQHLAQLI
                     DSLAKHGIAVRQP"
     gene            complement(5241..6767)
                     /locus_tag="VFB80_11235"
     CDS             complement(5241..6767)
                     /locus_tag="VFB80_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZN34388.1"
                     /translation="MKIAIGQLWQETNTLNPLPTTPADFEAFGLLRGADLVRQMAETN
                     ELGGFIQSVRRWPEQPEIVGLVRLPAWPGGRATAETFQWLQDELIGSLQRALPVDGVL
                     LALHGALAADEHPDVEGEILAAVRRVVGNVPVVATLDLHANVTPEMAAAADSLVLYHT
                     APHVDVFETGQRGAAVLRRILLEGARPVTALVKVPCVLPAERANTELAIGVSAELKRK
                     LQALESRPEVLTAGLATVQPWLDVPQLGSAVIVTTDGRPDLAEQACREIAAELWSRRA
                     EYLPELTSIEDAVRQAHESPAGLIVLSDAADATTSGAPGDSVWLLQELLKYEWPRPVL
                     LTLVAPEIVAAAERLGVGQTWSGQVGGVRDRRFGISIPITASVERLLDARFVLNGHLG
                     KNLPIDMGRAAVLRIGEIRIVVTARSGPHFAPELFQAAGHDPFAAAVVVAKSPAGFRA
                     VYAARAAAIYSVRAPGCAPSDFWKYEYQYRPRPLWPWEEIDGWQPTVEMFPSGRREQD
                     "
     gene            complement(6946..>8161)
                     /locus_tag="VFB80_11240"
     CDS             complement(6946..>8161)
                     /locus_tag="VFB80_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN34389.1"
                     /translation="FQRAEGCRAIDLDGRELIDLCCSHGATLLGHGDPRVRDALLDAL
                     ARGAACSYESELHAQLAELLCRSVPCLELVRFTGSGTEATMHCLRLARAFTGRQKILR
                     FEGNFHGYHDQVMWAAVGDAGQFAPQLKPISTGVAPSLGDQLVQIPYNRADLVEEAFA
                     RFGGELAAAILEPVYYNAGCIEPTAEFIQTLRRLTQQHGTVLIFDEVLSAFRMCLGGA
                     QEHLGVKPDLCTLGKAVGGGYPLSVFGGRRDIMQRLMPAGDCQHSGTYNGHPACVAAG
                     LAAVRAYGESGFYVHIHALCHRLVHGLREILARHGVTARVQGLGARIGIYFGLPADQE
                     IMSYADAQRHDRATLLRFYKLAIEHGVYFHDYGGAPCHHGFCAAMKMEDMDEALERLD
                     AVVAQLSGPEGR"
BASE COUNT         1364 a         2732 c         2685 g         1380 t
ORIGIN      
        1 agaagcacta gagaaccctc cgcggaatta aacttacccc tgcgccatta tacgcacagg
       61 cgtatattaa gcccttgcga tcctcgatca acttgccaat gacccgcacc gcaccgtcct
      121 attcgacggc gcattgccac aattgtatag atgcgccagc ctggcgattt attcccgccc
      181 gaatccagat ttttcgaaat cgtccgaggt cgccgatgat cccacacagc cggttggccg
      241 gcacaactac tcaagcattg tgcgacgctg ccagcttagt gagacaccga ttttgcgagc
      301 gacgtcgacg tcccaatcga cctcaaaacc ctcgttgcgc aggcattcgc atacctcgcg
      361 ggcgatgccg agcgcgttct tcgcgtaagc gtcgggagac atttgtgagt cgtcggccga
      421 aaaccagccg taagcaaggt agaccgacag acgggtgctg tcggccagca tatcgggcat
      481 gttttgatcg atgaaaaagg cgtagccacg cacacagtcg cgaaagcctg gatagcggcg
      541 gcttatcacg ttgacccgat cccctagttc ggcaccagta catgtgtcgc agcagggtga
      601 gacctgccac agcaggatgc ctcgttcgcg taaggcaact tcgactcggt caaggcgatc
      661 gcaatccgta acggccggcc aggcggcctt ttcggttgcc agcaggattg cggctcgctt
      721 gagttcgtcg gttgcgaagt ggatgaacgt gtcccattcg tcctcagggc tcagatcctg
      781 aatgtaagcc tcacagcagg cctcaaggat ttcttccttg tcgcgccgtg ccagccgaag
      841 ctcactgaga atgactctcc gcagcgtgtc gcgaagctcc tctcgcgact tgtcatccca
      901 gacttcctct ttgctcattg gtgtgttccc agcgatttag gctcgcattc gccgccctat
      961 tttcgcacct tggcgaaaaa cgcccgcacg tcttgggcca gcaactgcgg ctgctccagg
     1021 gcggcaaagt ggcccccctt tggcatttcg gtccagtgaa acagcgagcc gcccatcgcc
     1081 cgctcgaccc aggcccgcgg cgggacgttg atctccttgg gaaacaggac gaagcccatc
     1141 ggggcgggcc ggcccgtgct ctgaaacgcc gacatcgacg ccggccgcgg caggttgtgc
     1201 tgcgattcga agtagatccg cgtggcggag ggcatcgtct cggtcaccca atagatcatg
     1261 acgttggtca gcagctcgtc cttcgtgaag ctgttctcca gcttgccgcc gtggtcgctc
     1321 caggcccaga acttgtcgat gatccacgcc gccaggccgg ccggcgagtc gttcaggccg
     1381 tagccgagcg tcagcggccg cgagccctga atcgagctgt agccatactg gtcggccagc
     1441 tcgcggcggc gctgctgcat ccgctccatt tcctgcggag tgacgccgcg catcggatcg
     1501 tccattggct ggccgccgga ggggaagttg ctgtgcccgc cgatgcagtg gccgccgtcg
     1561 gccgacgcca gccagcgcgt gacgccgctg ccccagtcgc cgccctgcgc cccgtaccgc
     1621 tcgtagccca gccgggccat cagcttcgcg atcacctcgg ccatccgttg tgagctccag
     1681 ccgcgctcgg ccggcttgct cgaaaatccg aaccccggca gcgacgggca gacaacgtga
     1741 aacgcatcct cggccttgcc gccgtgagcg actgggtcgg tcagcgggcc gatgatcttc
     1801 tgaaactcga cgaatgaacc tggccagccg tgcgtgatca ccagcggcag agcgccgggc
     1861 tccttcgacc ggacgtggac gaagtgaatg tccaggccgt cgatccgcgt cgtgaactgg
     1921 ttcagctcgt tgagtttccg ctcctgccca cgccagtcgt agccggtccg ccaaaattcg
     1981 acgagcggct tcatctccga cagcggcacg ccgtattccc agccagtgcc ttcgatctga
     2041 tccggccagc gggtccgcgc cagccgctcc tgcagatcgc ggagcacgtc gtcgggcacg
     2101 gcaatctgga acggtttgat cgccgcctga tcctgggccg cggccaatcc cagcggcagc
     2161 agggcaatcg tcacgaccca gtagacgtcg gtcgtacggc gtttcatgca gcacctcgct
     2221 gatctgagtc gataacgtat cataaccaca tgcggtgcct cgccctgttc accggcggcc
     2281 tcgacagtca gctcgccgtg cggctgatgc agcggcaggg gatcgcggtg agggcaatct
     2341 actttcgctc ggcctggagc gatgcaactg ccgccgccac ggccgcagcc ggggcgctcg
     2401 acgtgcctct ggcggttgcc gatctgggcg aaaaatacgt ccagtcgctg cgccagccga
     2461 ggttcggata cgcctttggc gctgcgccgt gtctcgactg ccggattgcg atgctcagcg
     2521 aagctcgctg ccagctcgag cagcaggatg ccgaattcgt ggtaaccggg gaagtcgtcg
     2581 gccagcgcgt gcgttcggcg gtgcgcgacc tggagattgt tgcccatcat gcgggattgg
     2641 aagggcggct gctgcggccg ctctcggccc ggctgctgcc ggcaaccgaa tgcgaaagga
     2701 gtcactgcgt ggatcgcgcg cggcttttcg attggcaggg caagtcgcgg cggccgcaag
     2761 cgcagctggc ggcagaattg ggtctggcgg tcgttccgcc gcgaagcgat tgcccgctct
     2821 tgtcgggtcc gctcgccgag cgggcgctgg cgctagttcg ctctgggcag gggttgcggg
     2881 cctgggactt ggaactgctc gcgatcggcc ggcatgaggc ggttggcggc ggggcgcgcg
     2941 tcatcgtcgg gcgaaatgaa cacgaaaatg agcggctcac cgccacagcc gctggcgcgg
     3001 acgccggcaa tggtgcgctg gtttttcctg tgaatttcct tggtccgacg gcgctcttga
     3061 tcggcgcggc gaccgaagca aacgtacaac gtgcggccgc gctggtcgcg cgatacagtc
     3121 gcaacatgcc ggcctcgccg cggttgcggg tcgagacgag cggcgtcacg cgagagatcg
     3181 aattgtcgtc atgaatttag cgccgtcgcc gcccactaac ccgacgcgcc agcgagggag
     3241 tgccgtcttt ctcggcgtgc gactcgtgcc cgccctcgct tgcgcgtcgg gttggtgcct
     3301 gctcctggcg ctggccggct gcgatgccga gccggaagag acgtcgctga aagagcaggc
     3361 cgaagccgtc cgccgcggcg agagcgatcg catcctgatc gagacggcga tcctggccga
     3421 tgacgacctg cgcgagttga ccggcctgga aaatctgcgc gagctgctca tcgacagcgc
     3481 gagcagccgg ttcacggctg acggcatcgg gcagctgatt ggtctgccta agctggagca
     3541 tttgcgaatt cgcggaactg gcgtcgatga ccaagcgctc cagcacttcg ccggcctcga
     3601 gagcctcgtg atcctgaatc tgccgcaagg cacgttcggc gacgcggcat tggtggaact
     3661 gaagcggctg cccaagctcg cgcagctccg cttcagcagc ccgcgcgtga ccgacgccgg
     3721 catgcagacg ctggccgagc tgccggcgat caagcggctg catctgatcc atgtgccgat
     3781 caccgacgtg ggactggcgg aactgggaag aatcgagcgg ctcgagtcgc tgtacatcga
     3841 cggcggcgat ttttccgatt tcgcgatgga gaagctgttc cgcgagcggc cgcggctgca
     3901 tattcacctg aaccagcagc accacgatct cgatccgcac aagcactcgc actgatggcc
     3961 gccagcatca cgctcgccga catccgcgcc gcccgcgagc ggatcgccgg ggcgatctac
     4021 gtttcgccct gtccggagtc gccggcgctg tcggagctga cgggctcgcg ggttttctgc
     4081 aagctcgatt cgctccagcg gaccggcagc ttcaaggagc gcggggcccg caacgcgctg
     4141 ctgctgctgc ccgacgacaa aaagaaaaag ggcgtcgtcg ccgcttcggc cggcaaccac
     4201 gcgctggcgc tggcctgcca cggccgcgag ctgggcattc cggtaacggt ggtcatgccg
     4261 cagttcgcgc cgctcatcaa ggtggcgacc tgccggcggt tcggggcgaa cgtcgtgctt
     4321 tgcggcgaga atttcgctga agccaaggcc aaggccgacg agctggcggc ggccggcggc
     4381 ttgacctacg tccacggctt cgacgatccg gcggtgattg cgggccaggg cacggccggc
     4441 ctcgaaatcc tggagcaagt gccggggctg gaagcggtga tcgtgccggt cggcggcggc
     4501 ggtctgatcg cgggcgtctc gctggcgatc aaatcgctgc agccgcaggt ccgcgtgatc
     4561 ggcgtcgagg cggaggtgat gccgagcttt acgcaggccc tggccgcggg ccgcgcggtc
     4621 gtctgcccgg ccaggccgac gctggccgat ggtctggcca cgacgcggat gggcgacaac
     4681 gccttcgcca tcgccgaaaa gcacgtcgat cggctggtca ccgtctccga ggagcagatt
     4741 gccctctcga tcctgcggct gctcgagctg gaaaaatcgg tcgtcgaagg cgccgccgcc
     4801 gcgccgctgg cggcgctgtt gagcgggcaa ttgccggagc tgaagcgcaa gacagtcgcg
     4861 ctgttgctct gcggcggcaa tatcgacccg ctggtactgg ggcggatcat cgaagtcggc
     4921 ctggctgccg acggccgcct ggcccgcctg atcgccacga tcagcgatcg ccccggcggc
     4981 ctggctcacc tcgcgtcgat cattgcccag gccggcgcga gcgtgcagca gatcacgcac
     5041 gagcgcgtct tctccggtcc cgacgtcagc agcgtccgcg tgatctgcga cgtcgaaacc
     5101 cgcgaccgcc agcacctggc gcagctcatc gactcgctcg cgaagcacgg tatcgccgtg
     5161 cggcagccgt aggggattag gattaggatt aggactaggg cagagccagc tgcgtcctaa
     5221 tcttaatcgt aatcctaatc ctaatcttgc tcccgacgcc ccgacgggaa catctccacc
     5281 gtcggctgcc acccgtcgat ctcctcccac ggccacagcg gccgcgggcg gtactgatac
     5341 tcgtacttcc aaaagtcgct gggagcacag ccgggcgcgc ggacgctgta gatcgccgcg
     5401 gcccgggcag cataaacggc gcgaaacccg gcggggctct tggccacgac gacggccgcg
     5461 gcgaacggat cgtggccggc ggcctggaac agctcggggg caaaatgcgg cccggaccgc
     5521 gcggtgacga cgatgcggat ctcaccgatc cgcagcacgg ccgctcggcc catgtcgatg
     5581 ggcagattct tgccgaggtg gccgttcagg acaaaacggg catccagcag ccgctcgacg
     5641 ctggcggtaa ttggaatcga gatgccgaac cgccgatcgc ggacgccgcc gacctggccg
     5701 ctccaggtct ggccgacgcc cagccgctcc gcagcggcaa cgatctcggg cgcaacgagc
     5761 gtgagcagca ccggccgcgg ccactcgtat ttcagcaatt cctggagcag ccagacgcta
     5821 tcgcccggcg cgccgctggt ggtcgcgtcg gcggcatcgc tgagcacaat cagccccgcg
     5881 ggactttcgt gcgcttggcg aacggcgtct tcgatcgatg tcagctccgg caagtattcc
     5941 gcgcggcggc tccataattc cgcggcgatc tcgcggcagg cctgctcggc caggtcgggg
     6001 cggccgtcgg tcgtgacgat gactgccgag ccgagctgtg gcacgtcgag ccacggctgc
     6061 accgtcgcca ggcctgcggt cagcacttcg ggccgcgatt caagcgcctg gagcttccgc
     6121 ttcagctcgg cgctgacgcc gattgccaat tccgtattgg cccgctcggc gggtagcacg
     6181 cagggcactt tcaccagcgc ggtcaccggc cgcgcgcctt cgagcaagat tcgccgcagg
     6241 acggccgccc cgcgctggcc cgtttcgaat acatcgacgt gcggcgcggt gtgatagagc
     6301 accagcgagt cggctgctgc ggccatttcc ggcgtgacgt tggcgtgcag gtcgagcgtc
     6361 gccacgaccg gcacattgcc gacgacgcgc cgcaccgccg ccaggatttc gccttcgacg
     6421 tcgggatgct cgtccgccgc cagcgcccca tgcaatgcca gcagcacgcc atcgaccggc
     6481 aaggcgcgct gaagtgagcc gatcagctcg tcctggagcc actgaaacgt ctccgcggtc
     6541 gcccgcccgc cgggccaggc cggcagccgc acgagcccca cgatctccgg ctgctcgggc
     6601 cagcggcgga ccgactggat aaaacccccc agctcgtttg tctcggccat ctgccggacc
     6661 agatccgccc cgcgcaggag gccaaacgcc tcaaagtccg ccggcgtcgt gggcagcgga
     6721 ttgagcgtgt tggtttcctg ccagagctgg ccgatcgcga tcttcatgag gaaggcccgg
     6781 agggccgaca ggaaatagcc ccgaccgtga ggtcggggat ttgatggtaa agtccgcaga
     6841 ggcccggagg gccggcacaa cgggacttcg cgaagtgccg gccctccggg cctttgtatg
     6901 gttctgttta actccaaacc ccgacctgac ggtcggggct actacctacc ggccctccgg
     6961 gcctgacaac tgcgccacca cagcgtccag tctctccagc gcttcgtcca tgtcttccat
     7021 cttcatcgcg gcgcagaagc cgtggtggca gggcgcgccg ccgtagtcgt ggaagtagac
     7081 gccgtgttcg atcgccagct tgtagaatcg cagcaacgtc gcgcggtcgt gccgctgcgc
     7141 gtcggcgtag ctcatgattt cctggtcggc cggcaggccg aagtagatgc caatgcgtgc
     7201 gcccaagcct tgcacgcggg ccgtgacgcc gtggcgagct aagatttcgc gcaggccgtg
     7261 caccagccgg tggcagagcg cgtgaatgtg gacgtagaag ccgctttcgc cgtaggcccg
     7321 caccgcggcc aggcccgcgg ccacacaggc cggatggccg ttgtaggtgc cgctgtgctg
     7381 gcagtcgccg gcgggcatca gccgttgcat gatgtcgcgg cggccgccga acacgctgag
     7441 cggatagccg ccgccgacgg ccttgccgag tgtgcacagg tcgggcttga cgcccagatg
     7501 ctcctgggcc ccgcccaaac acatgcgaaa cgcactcagc acttcgtcga agatcagcac
     7561 cgtgccgtgc tgctgcgtca gccgccgcag cgtctgaatg aattcggctg tcggttcgat
     7621 gcagccggcg ttgtagtaga ccggctccag gatcgccgcg gccaattcgc cgccaaagcg
     7681 ggcgaaggcc tcctccacca ggtccgcccg gttgtacgga atctggacca gctggtcgcc
     7741 gagactcggg gcgacgcccg tggagatggg cttcagctga ggagcaaact gcccggcgtc
     7801 accgacagcc gcccacatga cctggtcgtg gtagccatga aagttccctt cgaaccggag
     7861 gatcttttgc cgcccggtga aggcccgcgc gaggcggagg cagtgcatcg tcgcctcggt
     7921 gcccgagccg gtgaagcgga ccagctccag gcagggcacc gaacggcaga gcagctcggc
     7981 cagctgggcg tgcagctccg actcgtacga gcaagccgcc ccgcgggcca gcgcgtccag
     8041 cagcgcgtcc cggacccgcg gatcgccgtg ccccagcagc gtcgccccgt ggctgcagca
     8101 gaggtcgatc agctcgcggc catccaggtc gatggcgcgg cagccctcgg cccgctgaaa
     8161 g
//
LOCUS       DASRJB010000426         7238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1812155, whole genome shotgun sequence.
ACCESSION   DASRJB010000426 DASRJB010000000
VERSION     DASRJB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812155"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFB80_11245"
     CDS             <1..928
                     /locus_tag="VFB80_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZN34390.1"
                     /translation="LCVLYVAMTRAVHALHLILAPAKANERSLPKTFGGLLRAALAEG
                     QPASGGRVLFELGDRQWWRTREGEAPAEPGRPVQAARRVARPPIALAPPAARRDRGLE
                     ITSPSALEGGARIDPADLLRPRAAGALGTGTLIHAWLEQIEWLDDGFPTDELLRQVAA
                     RLRAEIGGVYQQQADLAQRFRQQLAASHVAAVLHRSYYARHAADEPLRAIRERKFDIR
                     TGERLLGGSIDRLVVLKQAGRAIAADIVDFKTDEVAASDSRGLAKKVEFYRPQLEAYR
                     TATAQLLKLEPARVSARLVFLTAGVVREIEPE"
     gene            complement(929..1744)
                     /locus_tag="VFB80_11250"
     CDS             complement(929..1744)
                     /locus_tag="VFB80_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN34391.1"
                     /translation="MNLPQLAATAFLFILLAVPPVVLVIVLARSNMNPIQCCLWGAAY
                     LLSKLLWRAQWPGWLPLAEGQGAIIVCNHRSSVDPFFVQTGTSRKVHWLVAREYCEHP
                     AFRWFLSACEVIPVNRGGVDTAATKAAIRYVAEGGLVGMLPEGRINMTEEFMLPCRPG
                     AALVALKAKAVVVPCYIQGAPYRRYPWSPLLMPARVEVRFGRPVDLSDLFDREGESGV
                     VQTAMLRILRALAETAGRPDFQPQLAGRNWKPTQEELDAAMDAADERAGRPGG"
     gene            1942..3411
                     /locus_tag="VFB80_11255"
     CDS             1942..3411
                     /locus_tag="VFB80_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN34392.1"
                     /translation="MADLPSPPAEPFAKPAEATGWWSQPCGPREVLAISLPLIVQTAF
                     WSVMWFIDRLYLSWYSPEATAAALPGGMFHWVLICLPSGIASFVNTFVAQYHGAGRPK
                     RIGVAVKQGIWFGWATVPLFLLAVPLAPWIFALVAHTPQVQRLEVIYFQVLAFGAGAV
                     VISNAQSSFYTGRGFTGIVMLVNAAGTLLDICLEYAFIFGALGLPEMGIAGAALTTVI
                     SNWATVLMFWLLMRRPQEREPFGLDDNRFDWEIFRRLIRFGLPSGLPQLIEGGAFTVL
                     TNAVTAVSLVAGAATSLAFTINAVAFVPMIGLNIAVSTLVGQKLGENRPDLAARATWS
                     AMALAMLYTGLCAAMYLAVPDWFLTLHTAFADKATFPAVRQTTIQLLWFVALYCFFDA
                     MQIVLVGALRGAGDTRFVLINATTISFISIVTGLLLERHYRWQETGYGLYGWWWVMTI
                     WLFALGLSYLGRFIQGRWKSIRVIEPELPDGDLAAKSSA"
     gene            complement(3427..4860)
                     /locus_tag="VFB80_11260"
     CDS             complement(3427..4860)
                     /locus_tag="VFB80_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34393.1"
                     /translation="MLRVTRRHFLRDAQLGIGAIALSSLLARDGVAAPLPAAINPLAA
                     RQPHFPAKAKQVIYLHMTGSPPNLDLWDFKPELVKRSGEDCPETFLKGRTFAFTSGVP
                     KLLGTPRKFEQHGQGGQWMSDAVPNLHELADEMCMVHSMYTEQFNHAPAELLLYTGSP
                     RSGRPAMGAWVTYGLGSENEDLPGYVVLISSGVQPNGGKNSFGSGFLPSVFQGVQCRS
                     KGDPVLYASDPPGMDRELRRKSLDALRDLNELQAAELGHPETATRIAQYELAYRMQVA
                     VPEVMDITRESQQTLEAYGAKPGEASLANNCLLARRLIEQGVRFVQLFDWGWDFHGTN
                     PKEDIRDGLTTKCATMDRPVAALLKDLKQRGLLESTLVIWGGEFGRTPFREGRTAKGN
                     VLGRDHYPDCFTMWMAGGGARGGYSHGQTDELGFGIAQDKVHIHDLQATILHLLGFDH
                     TRLIHRFQGRDYRLTDVHGRVVKELLA"
     gene            complement(4885..5622)
                     /locus_tag="VFB80_11265"
     CDS             complement(4885..5622)
                     /locus_tag="VFB80_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN34394.1"
                     /translation="MSCETITRRNLPHWCVPCAIHFVTFRLAGSLPRDVIDGLKQRRR
                     ELLSKASAGVSASEHRQRAHKLLFAAYDDYLDSHRQIDWLLDPRIAALVRSSLYHLHG
                     KKIVLWAYSIMPNHVHALFAPMLGAENERQALDDEIGENEDRGPLAGIMHSLKGYTAH
                     QANKILGRSGAFWQHESYDHWIRDENELERVVDYISANAVKAQLAQRTTDWYWCSAHD
                     RFLTDGDSTGWLNLPLPSDLAVQSKLE"
     gene            complement(5701..>7238)
                     /locus_tag="VFB80_11270"
     CDS             complement(5701..>7238)
                     /locus_tag="VFB80_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34395.1"
                     /translation="QLTATAFHRNTQTNNEGGTNDEEYRNVAVVDRVNTTMAVWMGTT
                     IACAQCHNHKYDPISQEEFFRLFALFNNTADADRRDESPLLERWSPQQIEQREILEEE
                     IAAIEKEREEDKEKNKDRLAALKKSLAEIKPEKSVPIQRELPADQRRVTKLQHRGNYL
                     DVGQEVTAGTPAAFPPLPEDAQPAGGNTAQPAAGNTAPADRLALARWLVSPENPLTAR
                     VLANRFWEQIFGIGLVATSEEFGSQGELPSHPELLDWLATELERLNWDQKAFLKVLVM
                     SAAYRQSSRVTPELAARDPDNRLLARGPRVRLSAEMVRDQALAVSGLLSPKLFGPPVK
                     PPQPALGLSAAFGSGIDWQASKGEDKHRRALYTTWRRSSPYPSMTTFDAPNREVCTLR
                     RSRTNTPLQALVTLNDPVYIEAAQSLARRVVQGGAETGERIDFAFRLCLSRPPTAAEA
                     SRLIKLLDETLAEFRDDAESAKKMATDPLGAAPEGADLAQLAAWTVVANVILNLDEML
                     MKR"
BASE COUNT         1128 a         2388 c         2407 g         1315 t
ORIGIN      
        1 gctgtgcgtg ttgtacgtcg cgatgacgcg ggcagtccac gcgctgcatt tgatcctggc
       61 gccggccaag gcaaacgagc ggagcctgcc caaaaccttc ggcggcctgc tgcgagcggc
      121 gctggccgag ggtcagccgg ccagcggcgg gcgggtgctc ttcgagctag gggaccggca
      181 gtggtggcgg acgcgggagg gcgaggctcc tgccgagccg ggacgacccg tccaggcggc
      241 tcggcgggtg gctcgccctc ccatcgccct ggcgccgccc gcggcccgcc gcgatcgcgg
      301 actggagatc accagtccgt cggcgctgga agggggtgcg cgcatcgacc cggccgatct
      361 cctgcggccc agggcggcgg gtgcgctcgg cactgggacg ctgattcacg cctggcttga
      421 gcagatcgag tggctcgacg acggatttcc gacagacgag ctgctgcggc aggtcgccgc
      481 tcgactgcgg gcggaaatcg gcggcgtcta tcagcagcag gccgatctgg cgcagcgctt
      541 tcgccagcag ctagccgctt cccatgtcgc ggcggtcctg caccgctcgt attacgcgcg
      601 gcacgccgcc gatgagccgc tgcgcgccat ccgcgagcgc aagttcgaca tccgcaccgg
      661 cgagcggctg ctcggcggca gcatcgatcg cctggtcgtg ctcaaacagg ctggccgcgc
      721 gatcgctgcg gatatcgtcg atttcaagac cgacgaggtc gccgcgagtg actcccgagg
      781 actggccaag aaagtcgagt tctatcggcc gcagctcgaa gcctaccgca cggccaccgc
      841 gcagctcctg aagctcgagc cggcgcgcgt ctcggcgcgg ctggtgttcc tgactgccgg
      901 cgtcgtgcgg gagattgagc cggagtagct agccgcccgg ccggcccgcc cgctcgtcgg
      961 cggcgtccat cgcggcatcg agctcttcct gcgtcggctt ccagttgcgg ccggcgagct
     1021 gcggctggaa atcggggcgg ccggcggttt cggccagcgc gcgaagaatg cggagcatcg
     1081 ccgtctgcac gacgccggac tcaccctctc gatcgaagag gtcggacaga tcgaccggac
     1141 ggccgaaccg cacctcaacc cgcgcgggca tcagcagcgg gctccacggg tatcgccggt
     1201 agggagctcc ctggatatag cacggcacga cgacggcctt ggccttgagt gccaccagtg
     1261 ccgcccccgg ccggcacggc agcatgaatt cctcggtcat gttgatgcgg ccctccggca
     1321 acatgccgac cagtccgccc tccgcgacgt agcggatcgc ggctttggtc gccgccgtat
     1381 cgacgccgcc gcgattgacc ggaatcacct cgcaggccga gaggaaccag cgaaacgccg
     1441 gatgctcgca atactcgcgg gccaccagcc agtgcacctt gcggctggtg cccgtctgca
     1501 cgaagaacgg atcgacgctg ctgcggtgat tgcagacgat gatcgccccc tgcccctccg
     1561 ccagcggcag ccagcccggc cactgcgccc gccagagcaa cttggagagc aggtacgccg
     1621 cgccccacag gcagcactgg atcgggttca tgttgctgcg ggccagcaca atcaccagca
     1681 cgaccggcgg cacggcgagc agaatgaaaa ggaaggcggt cgcggcaagc tggggaagat
     1741 tcaagcgtcg tcctgcatcg gtttggaatt tccctactct acctttacgc gggcggcctg
     1801 gcagccttag ggtacagggc cgccgcgaac ctgttccctt gcggcgctgt ccgcactcga
     1861 ccgtccctcg ccttcgaccg acacacggag ccccgcctgt cttcctccac ggacatcccg
     1921 cttgcgcctg acgcgcccgc cttggccgac ttgccgtcgc cgcctgcgga gcccttcgca
     1981 aagcccgcgg aggcgaccgg ctggtggtcg cagccgtgcg ggccgcgcga ggtgctggcg
     2041 atttcgctgc cgctgatcgt gcagacggcg ttctggtcgg tgatgtggtt catcgaccgg
     2101 ctgtacttga gctggtactc gcccgaggca accgccgcgg ccctgccggg cggcatgttc
     2161 cactgggtgc tgatctgcct gccgagtggc atcgcgtcgt tcgtcaacac ctttgtcgcc
     2221 cagtaccacg gcgcgggtcg gcccaagcgg atcggcgtcg ccgtgaagca aggcatctgg
     2281 ttcggctggg caaccgtgcc gctgtttctg ctggcggtgc cgctcgcccc gtggatattt
     2341 gcgctggtcg ctcacacgcc gcaggtgcag cggctcgaag ttatctattt ccaggtgctg
     2401 gctttcgggg cgggtgcggt ggtgatctcc aacgcccagt cgagcttcta taccggccgc
     2461 ggcttcacgg gcatcgtgat gctcgtcaac gcggcgggga cgctgctcga tatctgcctg
     2521 gaatatgcgt tcatcttcgg agccctcggc ctgccggaaa tgggtatcgc cggcgcggcc
     2581 ttgacgacgg tgatctcgaa ctgggccacg gtcctcatgt tctggctgct catgcggcgg
     2641 ccgcaggagc gcgagccatt tggcctggac gacaaccgct tcgactggga gatcttccgc
     2701 cgcctcattc ggttcggcct gcccagcgga ttgccgcaat tgatcgaagg cggagcgttc
     2761 acggtcctga ccaacgcggt caccgcggtt agcctcgtcg ccggggcggc cacgtcgctg
     2821 gccttcacga tcaacgcggt cgccttcgtg ccgatgatcg ggctgaacat cgcggtcagc
     2881 acgctcgtcg gccagaagct cggtgagaac cgccccgacc tggccgcgcg ggcgacctgg
     2941 agcgccatgg ccctcgccat gctctacacc ggcctctgtg ccgcaatgta tctcgccgtg
     3001 cccgactggt tcctgacgct gcacaccgcg ttcgccgaca aggcgacttt tccggcggtg
     3061 cggcagacca cgatccagct gctgtggttc gtcgcgctgt attgcttctt cgatgccatg
     3121 cagatcgtcc tggtcggggc gctccgcggc gctggagaca cgcgatttgt gctcattaac
     3181 gccacgacca tttctttcat ctcgatcgtc accggcctct tactggagcg ccactaccgc
     3241 tggcaagaga ccggctacgg cctttacggc tggtggtggg taatgacgat ctggttgttt
     3301 gccctcgggc tgagctacct cggtcgcttc attcagggcc gctggaagag catccgcgtc
     3361 atcgagccgg agcttcccga tggcgacctt gccgccaaga gttctgccta gtcgcgcggt
     3421 tgcaattcag gccaacagct ctttgaccac gcggccgtgc acgtcggtca gccgatagtc
     3481 gcggccctga aatcggtgga tgagtcgcgt gtggtcgaag ccaagcaggt gcaggatcgt
     3541 ggcctgcagg tcgtggatat ggaccttgtc ctgggcgatg ccgaagccca gttcgtcggt
     3601 ctggccgtgg gagtaaccgc cgcgggcgcc tccgccggcc atccacatcg tgaagcagtc
     3661 gggatagtgg tcgcggccca gcacgttccc cttggcggtg cggccctcgc ggaacggcgt
     3721 gcggccgaac tcgccgcccc agatcaccag cgtcgattcc aagaggccgc gctgcttgag
     3781 gtccttcagc agggccgcca cgggcctatc catcgtcgcg catttggtgg tcaggccgtc
     3841 gcggatgtct tccttgggat tcgtgccgtg gaagtcccag ccccagtcga agagctgcac
     3901 gaaccgcacg ccctgctcga tcagccggcg ggccaggagg cagttgttcg ccaggctcgc
     3961 ctcgcccggc ttggccccgt acgcctccag cgtctgctgt gactcgcgcg taatgtccat
     4021 cacttcgggc acggcgacct gcatgcgata ggccagctcg tactgggcga tgcgcgtggc
     4081 cgtttcggga tggcccaact cggcagcctg cagttcgttg aggtcgcgaa gggcgtcgag
     4141 gctcttgcgg cgcagctcgc gatccatgcc cggcggatcg gaggcgtaga gcacagggtc
     4201 gcccttgctg cggcactgca cgccctgaaa caccgagggc aggaaaccgc tgccaaagct
     4261 gttcttgccg ccgttgggct gcacgccgct gctgatgagc acgacgtagc ccggcaggtc
     4321 ctcgttctcc gagcccaggc catacgtcac ccaggcgccc atcgccggcc ggcccgagcg
     4381 gggcgagccg gtgtagagca atagctcggc cggagcgtgg ttgaactgct cggtgtacat
     4441 ggaatgaacc atgcacatct cgtcggccag ctcgtgcaag ttcggaacgg cgtcggacat
     4501 ccactggccg ccctggccgt gctgctcaaa cttgcggggc gttccgagca gcttgggtac
     4561 gcccgaggtg aaggcaaacg tccggccctt caagaacgtc tccgggcaat cctcgccgct
     4621 gcgcttgacc agctccggct taaagtccca caagtccagg ttcgggggcg agccggtcat
     4681 gtgaaggtaa atgacctgct tggccttcgc cggaaagtgc ggctgccggg ccgcgagcgg
     4741 attgatcgcc gccggcagcg gcgcggcaac tccgtcgcgg gccagcagcg acgagagggc
     4801 aatcgccccg atgccaagct gcgcgtcgcg gaggaaatgg cggcgggtga ctcgtagcat
     4861 aagcgtcctc gcttgtgcaa ttctctattc aagtttggat tggactgcca ggtccgacgg
     4921 caacggtaaa ttcaaccagc cggtagaatc tccgtcggtc aggaaccgat cgtgagccga
     4981 acaccaatac cagtccgtcg ttcgttgcgc aagctgcgcc ttgactgcgt tcgcactaat
     5041 atagtcgacg acgcgctcca gctcattctc gtcgcgaatc caatggtcgt acgactcgtg
     5101 ctgccagaaa gccccgctgc gcccgaggat tttgttcgcc tggtgggcag tgtagccctt
     5161 gaggctgtgc atgatgccgg ctagaggacc tcgatcttcg ttctcgccaa tctcgtcgtc
     5221 cagtgcctgt cgctcgttct ccgcccccag cataggggcg aacaatgcat ggacatgatt
     5281 gggcatgatg gaataagccc acagcacaat cttcttgccg tgcaaatggt acagactgct
     5341 ccgcaccagc gccgcaattc gcggatctag cagccaatcg atttgtcggt gactatcaag
     5401 atagtcatcg taagcagcaa agagcagctt gtgagctcgc tgccgatgct cggaagcaga
     5461 gaccccggcc gaggccttcg acaacaactc tcgcctgcgc tgcttcagtc catcaatgac
     5521 gtctcgcggc agcgatccgg cgagccgaaa ggtgacgaaa tggatcgcgc acggcacaca
     5581 ccaatggggc aggttccgac gggtgatggt ctcgcaactc attggctcgg tgctcggggc
     5641 tgctgacgcg cggcgttcaa caaaatggag gcgtgagcaa gcgaggacgc ttgctctacg
     5701 ttagcgtttc atcagcatct catcgagatt cagaatgaca ttagcgacga cggtccaggc
     5761 ggccagttgg gcgaggtcag cgccctctgg cgcggcgccc agcggatcgg tcgccatctt
     5821 cttcgccgac tcggcatcgt cgcggaactc ggcgagcgtt tcgtccagga gctttatcag
     5881 ccgcgaggct tcggcggcgg taggcggccg cgacaggcag aggcggaagg caaagtcgat
     5941 ccgctcgccc gtttcagcgc cgccctggac gacccgccgc gcgagcgatt gcgccgcttc
     6001 gatgtagacc gggtcattca gcgtgacgag ggcctgcagc ggcgtgttgg tccgcgagcg
     6061 gcggagcgtg cagacctcgc ggttcggcgc gtcgaaggtg gtcatcgagg gatacgggct
     6121 cgagcgccgc caggtggtgt agagcgcccg gcgatgcttg tcctcgcctt tcgaagcctg
     6181 ccagtcgatg ccgctgccaa acgccgcgct cagtcccagc gcgggctgcg gcggcttcac
     6241 cggagggccg aacagcttcg ggctcaggag gccgctcacc gccagagcct ggtcgcggac
     6301 catctcggca gaaagccgca cgcgcgggcc gcgggccagc aggcggttgt cgggatcgcg
     6361 ggcggccagc tcgggcgtga cgcgcgacga ctgccggtag gccgccgaca tcaccagcac
     6421 tttcaaaaac gccttctggt cccagttcag ccgctcgagc tccgtggcga gccagtcgag
     6481 cagctccgga tgcgacggca gctcgccctg cgagccgaat tcctcgctgg tggcgaccag
     6541 gccgatgcca aagatctgct cccagaagcg gttggccaaa acgcgggccg tcagcgggtt
     6601 ctcgggcgac accagccagc gggccagcgc gaggcggtcg gccggtgcag tgtttccggc
     6661 ggcgggctgc gcagtgtttc cgccggcggg ctgcgcgtcc tcgggcagcg gcgggaaagc
     6721 ggccggcgtt ccggcggtca cttcctggcc gacgtcgagg tagttgcccc ggtgttggag
     6781 cttcgtcacc cgccgctgat cggccggcag ctcgcgctga atcggcaccg acttttccgg
     6841 cttgatctcg gccagggatt tcttcaacgc cgccaggcgg tccttgttct tctccttgtc
     6901 ctcttctctt tccttctcaa tggcggcgat ttcttcttcg aggatttccc gttgctcgat
     6961 ttgctgcggc gaccagcgct ccagcagcgg gctttcgtcg cgtcggtccg cgtcggccgt
     7021 attgttgaac agcgcgaaca gccggaagaa ctcttcctgc gagatcgggt cgtacttgtg
     7081 gttgtggcac tgggcgcagg caatggtcgt ccccatccag accgccatgg tggtgttgac
     7141 ccggtcgacg accgcgacgt tccggtactc ctcgtcgttg gtgccgccct cgttgttggt
     7201 ctgggtgttg cggtggaagg ccgtcgcggt gagctgct
//
LOCUS       DASRJB010000427         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_969921, whole genome shotgun sequence.
ACCESSION   DASRJB010000427 DASRJB010000000
VERSION     DASRJB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969921"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..630)
                     /gene="moaC"
                     /locus_tag="VFB80_11275"
     CDS             complement(130..630)
                     /gene="moaC"
                     /locus_tag="VFB80_11275"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZN34396.1"
                     /translation="MVSDLTHFDDTGASRMVDVGDKPATQRFARASGRVVMQPATLAR
                     IRQRPFAKGDVLEVARLAGIMAAKRTGELIPLCHPLPLDGVNIDFAFPDDRTVAITAE
                     VRVTAKTGVEMEALTAISVAALTIYDMCKAIDRGMSIERVQLDEKAGGKSGHFVREEQ
                     PPTAGT"
     gene            complement(674..1951)
                     /locus_tag="VFB80_11280"
     CDS             complement(674..1951)
                     /locus_tag="VFB80_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZN34397.1"
                     /translation="MNGLQTAALTDVGMRRATNQDSHALLLAETPEMFATRGHLLMVA
                     DGMGAHAAGELASKLAADGVPHLYHKYREFSAPEALQRAIRETNTEINRRGTASADFH
                     NMGTTSSVLVLLPQGALAAHVGDSRIYRLRGGTLSQLTFDHSLQWELQASGQLPAGTD
                     IAAVVPKNVITRSLGPNINVQVDLEGPHPIAPGDIYMLCSDGLTGRVEDAEIGAILSC
                     LPPQEAAQSLVDLANLRGGPDNVTVLVARIASKDLATGATHAEPLRIGASSERPAAHP
                     ATWIIGAVCLLIGIVLALTGRLIPAAVAVGIGAVAGLVGLVQRFGVAGGTVLGNGHML
                     GRGPYTSVACPPSHELALKLAATLDELRSAAREGNWDINWKPLDDACRQAQSADQAGN
                     FADAVRHYCRGISYMMKQLRLQNVRHGDNSVHD"
     gene            2222..>2462
                     /locus_tag="VFB80_11285"
     CDS             2222..>2462
                     /locus_tag="VFB80_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZN34398.1"
                     /translation="MRMLRDDSRHQWIRLALAAGLLAVCATSGCVRRRLTVRTFPPGA
                     QVYVDDQEIGTTPCSASFVYYGTRKITVMKDGYRTE"
BASE COUNT          399 a          808 c          817 g          438 t
ORIGIN      
        1 tcggcggcga tcggagcata aaggctgcga agcaagtcgg gcggtttcgt cgcggtgtgc
       61 atccgagtgg ggcaggcttt ccagcctgcc ataataggag catggcagac aggaatgtct
      121 gccccacttt cacgtacccg cagtcggcgg ctgctcttca cggacgaagt gcccgctctt
      181 gccgccggct ttctcgtcaa gctgcacacg ctcgatggac attccccggt cgatcgcctt
      241 gcacatgtcg taaatcgtta atgcagcgac acttatggct gtcaaagcct ccatctcgac
      301 gcccgttttg gcggtcaccc gcacttcggc cgtaatcgcc actgtgcggt catcggggaa
      361 ggcaaaatcg atgttcacgc cgtccagcgg cagcggatgg cagaggggga tcaactcgcc
      421 ggtccgcttg gccgccatga ttcccgccag gcgggcgact tccagcacgt cccctttggc
      481 gaagggccgc tgccggattc gggccaacgt cgccggctgc atcaccacac ggccgctggc
      541 ccgcgcgaat cgctgggtcg cgggtttgtc gccgacatcg accatccggc tagcgccggt
      601 gtcgtcgaaa tgggtcaaat cgctgaccat aagaatccat cataaccgca gccagaagcg
      661 cggcggagcg gcgtcagtcg tgcaccgagt tgtcgccgtg ccgcacgttc tgcaggcgga
      721 gctgtttcat catgtaagag atgccgcggc aatagtggcg gacggcgtcg gcgaaattgc
      781 cggcctggtc cgcggactga gcctgccggc aggcatcgtc gagcggcttc cagttgatgt
      841 cccagtttcc ttcgcgggcc gcggagcgga gctcgtccaa ggtggcggcg agcttcagag
      901 ccagctcgtg gctgggcggg caggcgacgc tcgtgtaggg cccgcgtccg agcatatgcc
      961 cgttccccag caccgtgccg ccggccacgc caaagcgctg caccaagcca accaggccgg
     1021 cgacagcgcc gatgccaacg gccaccgccg cgggaatgag gcggccggtg agcgccagca
     1081 cgatcccaat gagcaggcac accgccccga tgatccaggt cgccggatga gccgcgggcc
     1141 gctcgctcga agcgccgatc cgcagcggct cggcgtgggt agcacccgtg gcgaggtcct
     1201 tgctggcgat ccgggcgacg aggacggtca cgttgtcggg cccgccgcgg agattcgcca
     1261 gatcgacgag cgactgggcc gcttcctgcg gcggcaggca gctgaggatc gcgccgatct
     1321 cggcgtcttc cacgcggccg gtcaagccgt cgctgcacag catgtaaatg tcgcccggag
     1381 cgatcgggtg cggcccttcc aggtccacct gcacgttgat gtttgggccg agcgagcgcg
     1441 tgatgacgtt cttgggcacg accgcggcga tgtcggttcc ggccggcagc tgccccgaag
     1501 cttgcagctc ccattgcagg ctgtggtcga acgtcaactg gctcagcgtg ccgccgcgga
     1561 ggcgatagat acggctgtcg ccgacgtgcg cggccagcgc tccttgcggc agcagcacga
     1621 ggacgctgct ggtcgtcccc atgttgtgga agtcagcgct ggcggtgccg cggcggttga
     1681 tctcggtatt cgtctcgcgg atcgcccgct ggagtgcttc cggggcggag aactcccggt
     1741 acttgtgata gaggtgcggc acgccgtccg cggccagctt gctggccaat tcgcccgcgg
     1801 cgtgggcccc catgccgtcg gccaccatca gcagatggcc gcgggtcgcg aacatttcgg
     1861 gcgtttcggc aagcagcagc gcatgcgagt cctggttcgt cgcacggcgc atgccgacgt
     1921 cggtcaaggc cgccgtttgc aagccattca ctgctgccac cgtgaacgcc tttcgtcccc
     1981 cacgaagctg ccagggcggc gcacaacgcc gcaactgccc agcttcggta ttctatcggg
     2041 ctttcaagcg gccggtaatc agttttcgga gctcgattgc agccgcaaat cccgatggac
     2101 tatcaactta gtcgcggccg gctgcagggc aagccgaatt cgcggcgcga gcagttgctc
     2161 tgggccggtg gagcggtggt tcctatactc cggcctcttt ttcaccagtt ccggcgacag
     2221 catgcgcatg ttgcgagacg attcacgcca ccaatggatt cgcctggcgc tcgcggcggg
     2281 acttcttgcc gtctgcgcca ccagcggctg cgtgcggcgc cgactgacgg tccgcacgtt
     2341 tccgcccggc gcgcaggtct atgtcgatga ccaggagatc ggcacgacac cctgctccgc
     2401 gtcgttcgtc tattacggca cgcggaaaat caccgtgatg aaggacggct atcgcacgga
     2461 ga
//
LOCUS       DASRJB010000428         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2658195, whole genome shotgun sequence.
ACCESSION   DASRJB010000428 DASRJB010000000
VERSION     DASRJB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658195"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFB80_11290"
     CDS             <1..577
                     /locus_tag="VFB80_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN34399.1"
                     /translation="RDLAPEMPAAFQPKRPLVETILGGKPGEFEAVKDRLAQRLRDWA
                     KVAAEAEVVVAIKAHVSNAVQRPEQLLWLLEKVASPCMQAAYDYSHFELQGLAIQETM
                     TSLVPKSAFIHVKDTEHAQGKRGFLLPGRGSTDYVQYFKLLAASSYRGDVLVEVSSQV
                     SNQPGYDPLTAASQCYGHLADAMVRGGVKRA"
     gene            677..1237
                     /locus_tag="VFB80_11295"
     CDS             677..1237
                     /locus_tag="VFB80_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN34400.1"
                     /translation="MPKIFMPLGDATEAMDTFYPYFRLQEDGYEVVVAGPEARLYHTV
                     LHEIPPDSAVPWDITQERPGYFIKASLAFADAKGSDFAGAFVSGGRAPEYIRYNAELL
                     RVMREIHAAGKPIACVCHGIEILTAAGIIAGRTVTTVPKCRLDAEQGGAKYVDQSVVR
                     DGHFVTSRYWMDNAHLLREFLRMLAQ"
     gene            complement(1239..2024)
                     /locus_tag="VFB80_11300"
     CDS             complement(1239..2024)
                     /locus_tag="VFB80_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34401.1"
                     /translation="MGTPAPVNPYASPAPAPPGRPPDFRDLAVEESRRLIAAASGLPL
                     ERQPFSLCVPVENPDLTLLRKVKTGLVLRLVFGLAGLAGGGILAAIEEKGGFPPQLPD
                     ALILGVAVCSSLGGMLLLVSSATFFRRLVRRALGERFDQAQATGSAFGSLIVGVEDWS
                     TFRRMKLAPEDFACVFFDEAAACVRLEGARFRYFIRAADVVSLSEVAGASTVGTQIVY
                     RIGRTTLGITLQYDSFLHELRRQTIGARHTPLWTPLTTTLRRQ"
     gene            complement(2043..>2920)
                     /locus_tag="VFB80_11305"
     CDS             complement(2043..>2920)
                     /locus_tag="VFB80_11305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZN34402.1"
                     /translation="NKLKLYQDRLPPGSHRIPVVHLPKTIDNDYMGIDFTFGYFTAVE
                     TLAAEIRNLLYDAEASQAYFLTETMGRSAGWLAYGAAIAGEASLVISVEDIIGTYRVR
                     ETVTDEATGQTEEREVMDVERVVARIVKTMTARDAEGKHFGVIVLAEGLAELLPMKYL
                     KGIPRDEHGHISISHVNLGRMFAKLVSDEYKRQTGNPRKVTGLQLGYESRCSRPQAFD
                     VMLGSQLGVGAYRALVEEGLNGVMVSVVGQLQLHFVPFERLIDPQTLVTVVRYIEPNS
                     DFHRLARFLETYVNE"
BASE COUNT          514 a          987 c          937 g          482 t
ORIGIN      
        1 ccgcgacctc gctcccgaaa tgcccgccgc ctttcagccc aagcgcccgc tcgtcgaaac
       61 gatcctgggc ggcaagccgg gcgaattcga ggcggtcaaa gaccggctgg cccagcggct
      121 gcgcgactgg gcgaaggttg cagcggaggc cgaggtcgtg gtggcgatca aggcccatgt
      181 cagcaacgcc gtgcagcggc ccgagcagct gctctggctc ctggaaaaag tcgcaagtcc
      241 ttgcatgcaa gcggcttacg actacagcca tttcgagctc cagggcctgg caatccagga
      301 aacgatgacc tcgctggtgc cgaagtcggc cttcatccac gtcaaggaca ccgagcacgc
      361 ccagggcaag cgcggcttcc tgctgcccgg ccgcggctcc accgactacg tccagtattt
      421 caagctgctc gcggcgtcga gctatcgcgg cgacgtgctc gtcgaggtca gcagccaggt
      481 ctcgaaccag ccgggctacg atccgctgac cgcagcaagc cagtgctacg gtcacctggc
      541 tgacgcgatg gtcaggggcg gagtgaagag ggcgtgagtt gtcagtggtc agtagtcagt
      601 tgtcagtggc gaggggcgcg aatcgctgcg atactctcca ctgacaactg accactgact
      661 actgaccctc cccactatgc ccaagatctt catgccgctc ggcgatgcga ccgaggcgat
      721 ggatacgttc tatccgtact tccgccttca ggaggacggc tacgaagtcg tcgtcgccgg
      781 cccggaggcg cggctctatc acacggtgct gcacgagatc ccgccggact cggctgtgcc
      841 gtgggacatc acgcaggagc gtccgggcta cttcatcaag gcgtcgctcg cctttgcgga
      901 tgccaaaggc agcgacttcg ccggcgcgtt cgtctcgggc ggccgcgcgc cggagtacat
      961 ccgctacaac gcggagctgc tgcgcgtgat gcgcgagatt cacgccgccg gcaaaccgat
     1021 cgcctgcgtc tgccacggca tcgagattct caccgccgcc gggatcatcg ccggccgcac
     1081 cgtcacgacg gtgcccaagt gccgcctcga cgccgagcaa ggcggcgcga agtacgtcga
     1141 tcagtcggtc gtccgcgacg gccacttcgt cacctcccgg tactggatgg acaacgccca
     1201 cctgctccgc gagttcctgc ggatgctcgc ccagtaatct attggcgccg cagcgtcgtc
     1261 gtcagcggcg tccacagcgg cgtgtgccgt gcgccgatcg tctgccgccg cagttcgtgc
     1321 agaaagctgt cgtactgcag cgtgatcccc agcgtcgtcc ggccgatgcg gtagacgatc
     1381 tgcgtgccga cggttgacgc gccggcgacc tcgctcagcg aaaccacgtc cgccgcgcgg
     1441 atgaagtacc ggaaccgtgc gccttccagc cgcacgcagg ccgccgcctc gtcgaaaaag
     1501 acgcaggcga aatcctccgg cgctagtttc atccgccgaa acgtggacca gtcctccacg
     1561 ccgacgatca gcgagccaaa cgcgctgccg gtcgcctgcg cctggtcgaa tcgctcgccg
     1621 agggcccgcc gcaccagccg ccggaaaaag gtcgcactgg aaacgagcag cagcattccc
     1681 cccagcgatg agcagacggc gacgccgaga atgagcgcgt cgggcaattg cggcggaaaa
     1741 ccgcctttct cttcgatcgc cgccagaatg cctcctcccg cgagccctgc caggccgaag
     1801 accagccgca gcaccaggcc tgtcttgacc ttgcgcaaga gcgtcaaatc cgggttctca
     1861 accggcacgc acagcgagaa cggctgccgc tccagcggca gtccggacgc cgcggcgatc
     1921 agccggcggc tctcttcgac cgccaggtcg cgaaaatcgg gcggccggcc cggcggcgcg
     1981 ggcgccggcg aggcatacgg attgacaggc gccggcgtgc ccatattccg catccaccgc
     2041 cactactcgt tgacgtaagt ttccaggaac cgcgcgagcc ggtggaagtc gctgttgggc
     2101 tcgatgtagc ggacgacggt gaccagggtt tgcggatcga tcagccgctc gaacggaacg
     2161 aagtgcagct ggagctggcc gacgacgctg accatgacgc cgttgaggcc ttcctcgacg
     2221 agggcccggt aagctccgac gccgagctgg ctgccgagca tcacgtcgaa ggcctgcggg
     2281 cgagagcagc gcgactcgta gcccagctgc agacccgtga cctttcgcgg attgccagtc
     2341 tggcgcttgt attcgtcgct aaccagcttg gcgaacatcc ggccgaggtt gacgtgcgag
     2401 atcgagatgt ggccgtgctc gtcgcgggga atgcccttca agtatttcat gggcagcagc
     2461 tcggccagtc cttcggccag cacgatcacg ccaaaatgct tgccctcggc gtcgcgggcg
     2521 gtcatggtct tgacgatgcg ggccacgacc cgctcgacgt ccatcacctc gcgttcttcg
     2581 gtctgcccgg tggcctcgtc ggtcactgtt tcccggacgc ggtaggtgcc gatgatgtcc
     2641 tcgacgctga tcaccagcga cgcctcgccg gcgatcgccg cgccgtaggc cagccagccg
     2701 gcagagcggc ccatcgtctc ggtcaggaaa taggcctggc tggcttcggc gtcgtacagc
     2761 aaattgcgga tctccgccgc cagcgtctcg acggcggtga aatagccgaa cgtgaagtcg
     2821 atgcccatgt aatcgttgtc gatggtcttg ggcagatgca ccaccggaat gcggtgcgat
     2881 ccgggcggca ggcggtcctg atacagtttg agcttgttcg
//
LOCUS       DASRJB010000429         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1530212, whole genome shotgun sequence.
ACCESSION   DASRJB010000429 DASRJB010000000
VERSION     DASRJB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530212"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..755)
                     /locus_tag="VFB80_11310"
     CDS             complement(63..755)
                     /locus_tag="VFB80_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN34403.1"
                     /translation="MCRFAFLFVTSVALLAPWAAAADPAPNTLSEEEKSAGWKLLFDG
                     KSTAGWRNYKKTDVSPGWKVENGALVRAGQGAGDIITTDKFDAFEFSVEYNISKEGNS
                     GLMFHVTEDGGAPWHSGPEIQIQDNKDGHDPQLSGWLYQLYKPEIDATKPAGEWNQLR
                     ILITPEKCATWMNGKLYYEYVKGSKDWDERVAKSKFATMPAFGKATSGHICLQDHGNL
                     VSFRNIKIRPIK"
     gene            complement(852..>2052)
                     /locus_tag="VFB80_11315"
     CDS             complement(852..>2052)
                     /locus_tag="VFB80_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZN34404.1"
                     /translation="TAWSFHSAGQLVFGRGAVAQLGELVARRRLRSVLVVTDRRLATA
                     GLVERVLAPLAAAGIATHVFDEGEPEPSIAVALRAADAAARLAPDCVLGLGGGSNMDL
                     AKITAILITHGGPPSRYFGFGNVPGPVLPLVCVPTTAGTGSEVSHAAVLTDTENHIKV
                     STLSQFLRPALALVDPALTDSCPRQVTADSGIDALTHAIEAYTATDYQDLEAGPHEPV
                     AYEGRHPLGMCLAEKAIALIGEHLVTAVKDGANRAARDGMALAATVAGLAFSNCAVAL
                     VHALEYPLGAVLHCSHGAGNGLLLPHVMRYNLSARAESQAAIARLLGENVAGLSSAAA
                     AERAIAAVQRIARAIGIPQRIRDLGGNREQLPVFAEKAFAIKRLMNTNPRRPTQADLL
                     AILEAAF"
BASE COUNT          351 a          642 c          699 g          360 t
ORIGIN      
        1 gccgcgcggc tcggcaggag cctcgccctc ccggccgagg ccagcgcgac ccatagccgc
       61 ggctatttga tcggccgaat cttgatgttg cggaagctga cgaggttgcc gtggtcctgg
      121 agacagatgt ggccgctcgt cgccttgccg aacgcgggca ttgtggcgaa cttgctcttg
      181 gcgacccgct cgtcccagtc cttggagcct ttgacatact cgtaatagag cttgccgttc
      241 atccaggtgg cgcacttctc gggcgtgatc agaatgcgga gctggttcca ctcaccggcc
      301 ggtttggtgg cgtcgatctc gggcttataa agctgataga gccagcccga gagctgcggg
      361 tcatggccgt ccttgttgtc ctggatttga atttcggggc cgctgtgcca cggagcgccg
      421 ccgtcctcgg tgacgtggaa catgaggcca ctgttgcctt ccttcgagat gttgtattcg
      481 acgctgaact caaaggcgtc gaacttgtcc gtcgtgatga tgtcgcccgc gccctgacca
      541 gcccgcacca gcgcgccgtt ttcgactttc cagccgggac taacgtcggt cttcttgtag
      601 ttccgccagc cggcagtgga tttgccgtcg aagagcagct tccaaccggc ggatttttct
      661 tcttcgctga gtgtgtttgg ggcggggtcg gcagcagcgg cccaaggggc gagcagggcg
      721 acgctggtaa caaacaagaa ggcaaagcgg cacatggcgg cggctcctgg atcaagcggg
      781 ggaagtggga cgcccaccat catacgcagc cagcgtgcta gcccgcgagg gagggaaacg
      841 cgaatcggcg gctaaaaagc tgcctccagg atcgccagca aatcggcctg ggtcggccga
      901 cgcggattgg tgttcatcag gcgtttgatg gcgaaggcct tttcggcaaa cacgggcaat
      961 tgctcgcgat tgccgcccag atcgcggatc cgctggggaa tgccgatggc ccgggcgatg
     1021 cgctggacag cggcgatggc tcgctcggcg gccgctgcgg atgaaagacc ggccacgttt
     1081 tcgccgagca gtcgggcgat ggccgcctgc gactcagcgc gggctgacag gttgtaacgc
     1141 atcacgtgcg gcaacaggag tccattgccc gcgccgtgcg aacagtgcag cacggcgcct
     1201 agcggatact ccaaggcgtg gacgagcgca actgcgcagt tggagaatgc cagaccggca
     1261 accgtggcag ccagggccat gccgtcgcgg gccgcccggt tcgctccgtc cttcaccgcg
     1321 gtcacgagat gctcgccgat aagcgcgatg gccttttcgg ccaggcacat ccccagcgga
     1381 tgccgacctt cgtaggcgac cggctcgtgc gggccagctt ccaggtcttg atagtccgtc
     1441 gccgtatagg cctcgatggc atgggtgagt gcatcgatgc cgctatcggc cgtcacttgt
     1501 cgcgggcagc tgtcagtgag tgccggatcg acgagggcca gtgccggacg caagaactgg
     1561 cttagggtgc tgaccttgat gtggttctcc gtgtcggtga gaacggcagc gtggctcact
     1621 tcgctgccgg tgccggccgt agtcggcacg cagaccagcg gcagcaccgg tccgggcacg
     1681 ttgccgaagc cgaagtagcg gctcggagga ccgccgtggg tgatgaggat tgccgtgatc
     1741 ttcgccagat ccatgttgct gccgcccccc aggcccagca cgcaatcggg agccaggcgc
     1801 gccgccgcat cggccgcccg cagagccacc gcgatgctcg gctcgggctc accctcgtcg
     1861 aagacatgcg tggcgattcc cgcggcagcg agcggcgcca ggactcgctc gacgagtccg
     1921 gccgtcgcga gccgccgatc ggtcacgacc aaaacactgc ggagccgacg gcgggcgacg
     1981 agctcgccga gctgcgccac ggcgccacgg ccaaagacga gctgcccagc ggaatgaaaa
     2041 gaccaggcgg ta
//
LOCUS       DASRJB010000430         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2086988, whole genome shotgun sequence.
ACCESSION   DASRJB010000430 DASRJB010000000
VERSION     DASRJB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086988"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFB80_11320"
     CDS             complement(<1..1391)
                     /locus_tag="VFB80_11320"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN34405.1"
                     /translation="MRGAFRFAASVGTFLLLAAGAMAQQPRPAAPPAFPGPIEGGYLL
                     PNGWRVTPVGQQVLLTDLPLNILTSADGKYAFVATSGYNAHELTVVELASGRKVTNDT
                     ARQSWFGLAQNEATGQIWWSGGGGAVLHSFRWTGEKLEAGPSYPPAAAPDPNKPEEGP
                     PPGFRTGICLDAKSGTMYSLTILAKGGNKSFAWGDATTDENSGGGISRHNMKDEMGDM
                     SAPCGVRPYDVVLARNGLLYVSDWAKRVVLVVDPQSLRTVGRIPVGEHPNQLVLHPKD
                     DRLFVACASSNCVSVIDTKAGTVQETIYTALFPQSPEGSTPDALAIEPDGETLFVANA
                     DNNCVAVIDIEAPRRSQVKGFIPTGWYPTSVAVSRDSKQLLIGVGKGNQTASNKPEEA
                     RLAEALARPAGEGGYKRIPFPHVGTTLSGALSIVPMPSEEQLKKYTDQVYRNCPYSDK
                     QLTLAPSDRKTAIP"
     gene            complement(1498..2163)
                     /locus_tag="VFB80_11325"
     CDS             complement(1498..2163)
                     /locus_tag="VFB80_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34406.1"
                     /translation="MSATFKLPPTARDFDLFRAICVAGDSTRAAAAAFKISQTRVRQI
                     ITRVGHWLAESLPEMAEMATEKVLRLAEHLAADRLDYMYGQLMRMWTATSAPKYLAGA
                     ARMALALSRHGIVGGKIEALAADATLGPLPDDVWEEEASPPPVEDCSPAADPAPMAAA
                     PPAAVSAAKSSASGACSSPAATETSSDPTFQAMLRTLRSAELTPRSRRLGLAPQRLAP
                     ATS"
     gene            complement(2367..3926)
                     /locus_tag="VFB80_11330"
     CDS             complement(2367..3926)
                     /locus_tag="VFB80_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN34407.1"
                     /translation="MTARLSLFAAGAVFLAACLPLAAADRSNILFIMSDDHAYQAISA
                     YGSNRNQTPNIDRLAKEGMRFDRAFVTNSICGPSRAVIMTGKYSHLNGFPDNAARAWF
                     NRAQQTAPKLLQAAGYNTAVVGKWHLNSDPAGFDDWHILIGQGPYYNPRMKTPTGFVN
                     HEGYTTDIITDVALDWLKNKRDKTKPFFLMYQHKAPHRNWQPAPRHLDKYKDVKIPEP
                     ETLFDDYSGREKPAAENQMSVAKHLTPDDLKLSTPGGLNAEQAALFTKAYQKENDELK
                     AALLTPKQQTQWNYQRFVKDYLRCVDAVDESVGRVLDYLDESGLAQNTVVFYTSDQGW
                     YLGEHGWYDKRWMYEESFRTPLLVRWPGKTKAGSTTSQMVMNLDLAETFLDIAGLKVA
                     DDMQGRSIVPILAGEQPADWRKSVYYHYYEFPQPHHVHPHTGVRTERYKLIHFYTIDA
                     WELFDLEKDPHELKSVYRDPTYAGILTDMKAELTRLMAHYKDDGTTVAQFDNPDQPPP
                     MPKAKKAKQAK"
BASE COUNT          630 a         1238 c         1375 g          784 t
ORIGIN      
        1 ggaatcgccg tcttgcggtc gctcggcgcg agcgtgagct gcttgtcgga gtacgggcag
       61 ttgcgataga cctggtcggt gtacttcttc aattgctctt cgctgggcat cggcacgatc
      121 gacagcgccc cggagagcgt cgtgcccacg tgcgggaaag gaatccgctt gtagccgcct
      181 tcgccagccg gcctggcgag cgcctcggcc aaccgggcct cctccggctt gttggacgcc
      241 gtttggttgc ccttgcccac gccgatcagg agctgcttgc tatcgcggct gacggcgacc
      301 gacgtcgggt accagccggt cgggatgaaa cccttcacct ggctgcggcg cggggcttcg
      361 atgtcgatca ccgcgacgca gttgttgtcg gcgttggcca caaacagcgt ttcaccgtcg
      421 ggctcgatcg ccagggcgtc gggcgtgctg ccttcgggcg actgcgggaa cagcgcggtg
      481 tagatcgtct cctgcacggt gcccgccttg gtgtcgatca ccgaaacgca gttgctcgag
      541 gcgcaggcca cgaacagccg gtcgtccttc gggtgcagca ccagctggtt cggatgctcg
      601 ccgacgggaa tgcggccgac cgtccgcagc gactgcggat cgacgaccag caccacgcgc
      661 ttggcccagt cgctcacata gagcagcccg ttgcgggcca gcacgacgtc gtacggccgc
      721 acgccgcagg gcgcggacat gtcgcccatc tcatctttca tgttgtgccg gctgattccc
      781 ccgccgctgt tttcgtccgt cgtcgcgtcg ccccaggcaa aggacttgtt gccacctttg
      841 gccaggatcg tgagcgagta catggttccg ctcttggcat ccaggcagat gccggtgcga
      901 aatcccggcg gcgggccttc ctccggcttg ttggggtccg gggcggcagc cggcggatag
      961 gacgggccgg cttccagctt ttcgccggtc cagcggaacg agtgcagcac ggctccgccg
     1021 ccgcccgacc accagatctg cccggtggcc tcgttctggg ccagcccaaa ccagctttgg
     1081 cgggcggtgt cattggtgac tttgcggccg ctcgccaatt caacgacggt cagctcgtgg
     1141 gcgttgtagc cgctcgtcgc tacgaaggcg tacttgccgt cggccgacgt gaggatattg
     1201 agcggcaagt cggtgagcag cacctgttgc cccaccggcg tcacgcgcca gccgttgggc
     1261 agcaggtagc cgccttcgat cgggccggga aacgccggcg gcgccgcggg ccttggctgc
     1321 tgcgccatag cgccggcagc caagagcaag aaagttccaa cgctggctgc gaaccgaaat
     1381 gcaccacgca tggcttgcct cagtgaggtg aacgggttcg atcgttctgt gcgcggacta
     1441 tcgtagccgc tgctggtgag cggaggtact ctgcgcgggt gaagattcga taaagcttca
     1501 ggacgtggcc ggcgcgagcc gttgtggcgc gaggccgagc cggcgagatc gcggcgtgag
     1561 ctcggcgctg cgcagggtcc gcagcatcgc ctggaacgtc ggatcgctgc tggtttcagt
     1621 tgccgctggc gagctgcaag cgccgctggc ggaagacttt gcggccgaaa cagcggcagg
     1681 aggcgctgcg gccatggggg cgggatcagc cgcaggtgag cagtcctcga cagggggagg
     1741 cgaggcttcc tcttcccaaa cgtcgtcggg caacgggcca agggtggcgt cggccgccag
     1801 cgcctcgatc ttgccgccga cgatgccgtg ccgggaaagc gccaaggcca tccgcgcagc
     1861 cccggcgaga tatttcggcg cgctcgtcgc ggtccacatc cgcatcaact gcccgtacat
     1921 gtaatccagc cggtcggctg ccaggtgctc ggccagccgc agcactttct cggtcgccat
     1981 ctcggccatt tccggcagcg actccgccag ccagtggccg acgcgggtga tgatctggcg
     2041 tacgcgggtc tgcgaaatct tgaacgcggc agcagccgcg cgggtcgaat cgcccgcgac
     2101 gcaaatcgcc cgaaacagat cgaaatcccg cgccgtcggc ggcagcttga acgtggcact
     2161 catcgctgaa tctcccagta caaaagtttt tgtcgcgtgg gcctggccca ccatcgcgcg
     2221 tttgcgcgac gcaagatcgt acggggaggg atggactagt ctggtccatt tgagtcaagc
     2281 gaaaaaaaga attatcgaaa tagttcgcgg cctagcctcg ttcccacgct ccgcgtgaga
     2341 accaggtggc gaacgcgaag ccgctgctat tttgcctgct tggccttttt ggccttgggc
     2401 attggcggcg gctggtcggg gttgtcgaac tgggccacgg tcgtgccgtc gtctttgtaa
     2461 tgggccatca gccgcgtcag ctcggctttc atgtccgtga ggatgcccgc gtaggtagga
     2521 tcgcgataga cgctcttcaa ttcgtgcggg tccttctcca ggtcgaacaa ctcccaggcg
     2581 tcgatcgtgt agaagtggat cagcttgtag cgctcggtcc gcacgccggt gtgcgggtga
     2641 acgtggtgcg gctgtggaaa ctcgtagtag tgataataga cgctcttccg ccaatccgcc
     2701 ggctgctcgc ccgcgagaat cggcacgatg ctgcggccct gcatgtcgtc cgcaactttc
     2761 aggccggcga tgtcgagaaa cgtctcggcc aggtcgagat tcatcaccat ctggctcgtg
     2821 gtgctgccgg ccttggtctt gccgggccag cggacgagca gcggcgtgcg gaacgactct
     2881 tcgtacatcc accgcttgtc ataccagccg tgctcgccca ggtaccagcc ctggtcggag
     2941 gtgtaaaaga cgacggtgtt ctgggccagg ccgctttcgt cgagatagtc gaggacgcgg
     3001 ccgacgctct catcgacggc atcgacgcag cgaaggtagt ccttcacgaa ccgttgatag
     3061 ttccactgcg tctgctgctt gggcgtgagc aaggcggcct tcagctcgtc gttttctttc
     3121 tggtaggcct tcgtgaacag agcggcttgc tcggcgttca ggccgcccgg cgtcgaaagc
     3181 ttcagatcgt cgggcgtgag gtgtttggcg acgctcatct ggttttcggc cgccggcttc
     3241 tcgcggcccg agtagtcgtc gaacagcgtt tccggctcgg ggatcttcac gtccttgtac
     3301 ttgtccaagt gccgcggggc aggctgccaa ttgcggtgcg gcgctttgtg ctgatacatc
     3361 aggaagaacg gcttcgtctt gtcgcgcttg ttcttcagcc agtcgagcgc gacgtcggtg
     3421 atgatgtcgg tcgtgtaccc ttcgtggttg acgaaacccg ttggcgtctt cattcgcggg
     3481 ttgtagtacg gcccttgccc gatcaggatg tgccagtcgt caaagccggc cgggtcgctg
     3541 ttgaggtgcc acttgccgac gaccgccgtg ttgtagccgg cggcctgcag cagcttgggc
     3601 gcggtctgct gggcccggtt gaaccaggcc cgggcggcgt tgtcggggaa gccgttgagg
     3661 tggctgtact tgccggtcat gatcacggcc cggctcggcc cgcagatcga gttggtgacg
     3721 aacgcccgat cgaaccgcat gccctctttc gccaggcggt cgatgttcgg cgtctggttg
     3781 cggttgctgc cgtaggcgct gatcgcctgg taggcatggt cgtcggacat gatgaacagg
     3841 atgttcgagc ggtcggcggc ggcgagtggc aagcaggctg ccaggaaaac ggccccggcg
     3901 gcgaatagac tgaggcgggc tgtcatagga aggccaaggg gagagggcga ttggacgacg
     3961 cgaagatcgg ccgcgtcgcg gcccaacata ctgcggccga attggcgggg caacccgcgc
     4021 gcgaggt
//
LOCUS       DASRJB010000431         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3785532, whole genome shotgun sequence.
ACCESSION   DASRJB010000431 DASRJB010000000
VERSION     DASRJB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785532"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFB80_11335"
     CDS             complement(<1..1002)
                     /locus_tag="VFB80_11335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34408.1"
                     /translation="MASSPKSVKAVFEQAVEIPSPDERQAFLEQACAGDPNLRQRVEG
                     LLRAYADAGSFLESPPLGPTETAGDNSPGEAAGTMIADRYKLLEEIGEGGMGTVWMAQ
                     QTEPVKRTVAVKLIKPGMDSRQVLARFEAERQALALMDHPNIAKVFDGGITGEPGCVS
                     AARPYFAMELVKGVPITKYCDEHHLTPRQRLELFVPVCHAIQHAHQKGVIHRDLKPSN
                     VLVALYDDKPIPKIIDFGVAKAAGQQLTEQTLHTGFGAVVGTVEYMSPEQASFNALDV
                     DTRSDIYSLGVLLYELLAGSPPFGRKELERAGMLEMLRVIREQEPSKPSTKLSTADGL
                     "
     gene            complement(1069..1662)
                     /locus_tag="VFB80_11340"
     CDS             complement(1069..1662)
                     /locus_tag="VFB80_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN34409.1"
                     /translation="MPAVTQLLDAAAAGDPAAAAQLLPLVYNELRTLAAARMAAERSD
                     HTLSATALVHEAYLRLVARPGGEAGGEVLAWNSRGHFFAAAAEAMRRILVESARRKGR
                     LRHGGGRRRLDLEQVAVLHDDQNDELLALDEALGRLDAEEPETAAVVKLRYFAGLTTE
                     QAAAALDISLRTANRHWAYARAWLFQALGGDSEEKSV"
     gene            complement(1796..>2942)
                     /locus_tag="VFB80_11345"
     CDS             complement(1796..>2942)
                     /locus_tag="VFB80_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN34410.1"
                     /translation="AAAGHLRDYLSSRPDNADAHFLLARTLRRDGQFDEAATHLVDAQ
                     RLGWDPEAIRRETFLLRLQRTGVREASTDELLALVPDTNADRNLLEALYRGDGAIGNW
                     DRAALWLHAWLEDYPNDWAPRLWQAEILERFRKYDRARADYLRVLELQPDQPRALLGA
                     GQVALAHRADYVEAETYLGRYRAIDPAHQDARLGLALCRYGRGDLASARQMAEAVLAD
                     NPSHPGAALLLGSLEAEDGRDQEAIRWLKVAETAGADPQGVSYQLAQVLRRTGQTAEA
                     DRYERRFTELRDAQRALEPATRAAEAEPNNADRQYEVGRLYAVLGASDAAVQWFHKAL
                     TVDPAHRPSHTALADYYSRQGGPQAAARADFHRRQAQAEPAGPPRTE"
BASE COUNT          423 a          980 c         1021 g          518 t
ORIGIN      
        1 caagccgtcc gcggtgctca gcttggtgct cggcttcgac ggctcctgct cgcggatcac
       61 ccgcagcatc tccagcatgc cggccctctc cagctccttg cggccgaacg gcgggctgcc
      121 ggcgagcagc tcgtacagca gcacgccgag cgagtagatg tcgctccgcg tgtccacgtc
      181 gagagcgttg aagctcgcct gctccgggct catgtactcc acggtgccga ccacggcccc
      241 gaacccggtg tgcagggtct gctcggtgag ctgctgcccc gcggccttcg ccaccccgaa
      301 gtcgatgatc tttgggatcg gcttgtcgtc gtagagcgcg accaggacgt tggacggctt
      361 cagatcgcga tggatgaccc ccttctggtg ggcgtgctgg atcgcgtggc agacggggac
      421 gaacaactcc agtcgctgcc gcggggtcag gtggtgctcg tcacagtact tcgtgatcgg
      481 cacccccttg accagttcca tggcgaagta gggtctcgcg gcgcttacgc aaccgggctc
      541 gccagttatg ccgccgtcga aaaccttggc gatgttcggg tggtccatca gggccagggc
      601 ctgccgctcg gcctcgaatc gggccagcac ctgccgggag tccatccccg gcttgatgag
      661 tttgaccgcc accgttcgtt taaccggctc ggtctgctgg gccatccaga ccgtgcccat
      721 gccgccttcg ccgatctctt ccaacagctt gtagcggtcg gcaatcattg tcccggccgc
      781 ctcgccaggt gagttgtcgc cggcagtttc ggtggggccg agtggaggcg actcaagaaa
      841 gctgcccgcg tcggcatagg cgcgcaacag gccctcgact cgctgccgca gatttgggtc
      901 gccagcacag gcctgttcca ggaatgcctg gcgctcgtcc ggcgacggaa tctcgacggc
      961 ctgctcgaag acagccttga ccgatttcgg cgaagaggcc atgggaacgt ccggagtcgg
     1021 agatcgttca ccccccagtg cgacgggagg gtggaaagtg tgccaccgtc aaacagactt
     1081 ttcttcggaa tcgccgccga gtgcctgaaa gagccaggct cgggcatatg cccagtgccg
     1141 attggctgtt cggagcgaga tgtccagagc cgcggcggcc tgctcggtgg tcaacccggc
     1201 gaagtagcgg agcttgacga ccgcggcggt ttccggttcc tccgcgtcca ggcggccgag
     1261 ggcctcgtcg agcgcgagca gctcatcgtt ctggtcgtca tgcaagacag cgacttgttc
     1321 gaggtcgagc cggcgccggc cgccgccatg tcgaagccgg cccttgcggc gggcgctctc
     1381 gacgaggatt cgccgcatgg cttcagccgc cgcggcgaaa aagtggccgc ggctattcca
     1441 ggccaagact tcacccccag cttcgccgcc ggggcgcgcc accaggcgta ggtacgcctc
     1501 gtggacgaga gcggttgcgc tgagcgtgtg gtcggagcgt tccgctgcca tgcgcgccgc
     1561 ggcgagagtg cgcaattcgt tgtagacaag cggcaacagt tgggccgcgg ccgcgggatc
     1621 gccggcggca gctgcgtcca ggagttgggt gacagccggc atccgcacca gagtacttgc
     1681 ctccgatgtg aaccgcaagc attgcccgag ttgggtcgat atagctgagg atttcgatac
     1741 gcgacaatgg ccgacagccg agaagcaggg tccgcttttc gttcgctttc gcgctttact
     1801 cggttcgcgg cgggccagcc ggctccgcct gcgcctgccg gcggtggaag tcggcgcggg
     1861 ccgcggcctg cgggccgccc tgacgcgagt aatagtcggc aagggcggtg tggctcggtc
     1921 ggtgggccgg gtctaccgtc agcgctttgt ggaaccactg cactgccgcg tcggatgcgc
     1981 cgagcaccgc gtaaagccgg ccgacctcgt actggcggtc ggcattgttc ggctcggcct
     2041 ccgcggcccg ggtcgccggc tccagggcgc gctgggcgtc acgaagttcg gtgaagcgtc
     2101 tctcgtagcg gtctgcttcg gccgtttggc ccgtgcgccg gagcacctgg gcaagctggt
     2161 aactgacccc ctgcgggtcg gcgcccgcgg tctccgccac tttcagccac cggatcgcct
     2221 cttgatcgcg accgtcctcc gcctcgagcg acccgagtaa caacgctgcg ccgggatggc
     2281 tgggattgtc ggccagcacg gcttcggcca tctgacgggc gctcgccagg tcaccccggc
     2341 cgtagcggca cagtgccagg ccgagccggg cgtcctggtg ggccgggtca atggcgcggt
     2401 accgaccgag gtacgtctcg gcttcgacgt agtcggcccg gtgcgcgagc gcgacctgcc
     2461 cggcgccgag cagcgctcgc ggctggtccg gctgcagttc caaaacccgc agatagtcgg
     2521 cccgcgctcg gtcgtacttt cggaatcgct ccaggatttc cgcctgccac agtcgcgggg
     2581 cccagtcgtt tgggtaatcc tccagccagg cgtgcagcca cagcgcggcg cggtcccagt
     2641 tcccgatcgc cccgtcgccg cggtagaggg cttccagcag gttgcggtcc gcattggtgt
     2701 ccggcaccag ggccagaagc tcgtccgtcg atgcctctcg tacccctgtc cgttggagcc
     2761 gcaagaggaa ggtctctcgg cggatggcct ccgggtccca gccgagccgt tgggcatcga
     2821 ccagatgcgt cgcggcctcg tcgaactgcc cgtcgcggcg gagtgtccgg gccaggagga
     2881 agtgggcgtc ggcgttgtcc gggcgggagg acaggtagtc gcgcaggtgg ccggcggcgg
     2941 cc
//
LOCUS       DASRJB010000432         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1813368, whole genome shotgun sequence.
ACCESSION   DASRJB010000432 DASRJB010000000
VERSION     DASRJB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813368"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>2228
                     /locus_tag="VFB80_11350"
     CDS             193..>2228
                     /locus_tag="VFB80_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34411.1"
                     /translation="MKYQELLILLPCHSLEDFPTYHEGDDAQSLLASWSALWHPALIA
                     SAGQAPAWRRIDDPINDVRERLIVVPSICLQRLPTGFAQRVKDEGGVLIRKTGSREEI
                     LQAALEPLGMSPHGNATEGGNATAGIPYSDDFLALGYCYLMIELLTRQMRYSSNLDET
                     YFKGAVVAAAQAAVAGNAALAREKLAACFSVLAEERDHYYPVDAFVLDLNLAASSTLG
                     QSLRDELARPTPTNLLIKADLVAEMAAKEPATLAALKEARAAGRVGLIGGEASEERLP
                     LLSHESILAELLRGLAQYESLLGSRPAIYGRWRYGPTPHLPGVLHKLGFKGALHAGFE
                     EGKVPDGLQFKVRWEGLDGSALDAIARTPLDASKPQTFLGLATKLGESMDADHVATLC
                     LAHWPGQASSWLDDLRRISRYCAALGKWVTLDEYFAKTDQPGQLDRFEADRYRSPYLK
                     QAIIRRQDDPISTPVRYWRDQAAGGGARATSALAAFVSGKVLEEASGGREPTESAAAS
                     LAAALTAANPSGPKGTLILNPASFIRRAGVEELALEGLPTIERPVYAAEQAAGGLRAV
                     VDVPAFGFVHIAAGQPAAKEKKPIVLAADNTLRNEFFEAIINPTTGSLASIHEYQTRG
                     NRLSQQLALRSPGAKQKPGDTYRDPDEAADYSVMAADSVKTSIATTTLGEIVTRG"
BASE COUNT          393 a          755 c          739 g          341 t
ORIGIN      
        1 cgtaatcggc gggctgggcc aggcgggtct caagcatccg acaacggaac cggttcggga
       61 ttaggattag gatttagatt aggattagga ccaagacaaa gatgtagcat ccgatcctaa
      121 tcttaatcct gatcctaatc gtaatcctgc ctgctccgcc cgccctccca gcccggcgaa
      181 ctccctcctt gcatgaagta ccaagaactg ctgatcctcc tcccctgcca cagcctggag
      241 gattttccga cgtaccacga gggggacgac gcccagagcc tgctcgccag ctggtcggcg
      301 ctgtggcatc cggcgctgat cgcctccgcg ggacaggccc cggcctggcg gcgaatcgat
      361 gacccgatca acgacgtccg cgagcgcctg atcgtcgtgc cgtcgatctg cctccagcgg
      421 ctgccgaccg gcttcgccca gcgggtcaaa gacgaaggag gcgtgctgat tcgcaagacc
      481 ggcagccgcg aggagattct ccaggcggcg ctggagccgc tgggtatgtc gccacacggg
      541 aacgccaccg agggcggaaa cgccacagcg ggcattccct acagcgacga ttttctggcg
      601 ctgggttact gctacttgat gatcgagctg ctgacgcggc agatgcgcta ttcgagcaac
      661 ctcgacgaga cctatttcaa gggcgccgtc gtggccgcgg cgcaggcggc ggtggcaggc
      721 aacgccgcac tggcccgcga gaagctcgcg gcctgcttca gcgtgctggc cgaagagcgc
      781 gatcactact atcccgtcga tgcattcgtg ctcgacttga atctggcggc gagctcgacg
      841 ctggggcagt cgctgcgcga cgaactggcg cggccgacgc ccacgaacct gctgatcaag
      901 gccgatctgg tggccgagat ggccgccaag gagccggcca ctctggccgc cttgaaagag
      961 gcccgggccg ccggccgcgt cggactcatc ggcggcgagg ccagcgaaga acggctgccg
     1021 ctcctgtcgc acgagtcgat cctggccgag ttgctgcgcg ggcttgcgca gtatgaatcg
     1081 ctgcttggca gccggccggc catctatggc cgctggcgat atggcccgac gccgcacttg
     1141 ccgggagtcc tgcacaagct tggcttcaag ggcgcgctgc acgccggctt cgaggaaggc
     1201 aaggtgccgg acgggctgca gttcaaggtc cgctgggaag ggctcgacgg cagcgcgctc
     1261 gacgccatcg cccgcacgcc gctggacgcc agcaagccgc agacattcct cggcctggcc
     1321 acgaagctgg gcgagtcgat ggacgccgac cacgtggcga cgctctgcct ggcccactgg
     1381 cccgggcaag ccagctcgtg gctcgacgac ctgcggcgga tcagccgcta ctgcgccgcg
     1441 ctcggcaaat gggtcacgct cgacgagtac tttgccaaga ccgatcagcc ggggcagctc
     1501 gaccggttcg aagccgaccg ctatcgctcg ccgtacctga agcaggcgat catccgccgg
     1561 caggacgatc cgatatccac gcccgtgcgc tattggcgcg accaggcggc cggcggcggg
     1621 gcgagggcga ccagcgcgct ggcggccttc gtgagcggca aggtcctgga ggaggcgagc
     1681 ggtgggcgtg agcccactga gtcagccgcg gcgagcctcg ccgccgcact gaccgcggcg
     1741 aatcccagcg gccccaaggg gacgctgatt ctcaacccgg ccagcttcat ccgccgcgcc
     1801 ggtgtggagg agcttgcgct ggaaggcctg ccgacgatcg agcgccccgt ctatgccgcc
     1861 gagcaggccg ccggcggcct gcgcgcggtc gttgacgttc cggcattcgg cttcgtgcat
     1921 atcgcggccg gccagcccgc ggccaaggaa aaaaagccga tcgtcctcgc cgccgacaac
     1981 acgctgcgga acgaattctt cgaggcgatc atcaatccca cgacgggctc gctggcttcg
     2041 attcacgaat accagacgcg gggcaaccgg ctgtcgcagc agctggccct gcgctcgccg
     2101 ggagcgaagc agaagccggg cgacacctac cgcgatcccg atgaagcagc cgactattcg
     2161 gtgatggccg ccgattccgt caaaacctcg atcgctacga ctacgctcgg cgaaattgtc
     2221 acccgcgg
//
LOCUS       DASRJB010000433         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_683662, whole genome shotgun sequence.
ACCESSION   DASRJB010000433 DASRJB010000000
VERSION     DASRJB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683662"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFB80_11355"
     CDS             complement(<1..212)
                     /locus_tag="VFB80_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN34412.1"
                     /translation="MADVGGVAKRIIANVERAIVGKRPQLILSLTAWLCEGHILLEDV
                     PGVAKTMLARALARSVGCTFKRIQCTP"
     gene            252..1625
                     /locus_tag="VFB80_11360"
     CDS             252..1625
                     /locus_tag="VFB80_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN34413.1"
                     /translation="MPIRNFGRNLEFSPRQGHTPASEQELLEILRQYRGRQIRAVGRL
                     HSWSQAIVADDVLLDLRQLDAVRVEQRGGETWAVVGAGCQIKRVLSELDRHGVTLPTL
                     GLISEQTIAGAAATGTHGSGRHSLSHYLDEVRIAAYDAAGEPVIRTITGGDELRAARC
                     SLGCLGIVVSVAFRCRLKYHIEEHFREYDSLDDVLAAEAEYPLTQFYLVPWRWKWMAQ
                     HRRESAAPRSWLAPLYRWYWFLWIDVMLHLNVLLLVRWLRIAGLIKLFYRWIVPLAIV
                     RPWRVVDRSQDMLLMEHELFRHLELEVFVRRSQLAAATDFVRQTLQFFGGEAVPPLPQ
                     GEGTAGSYVHHYAICIRQVLPDDALISMASGGEEPHYAISLITYARPSERAGFLACAD
                     FLAHRMAALFDARPHWGKYCPLDAAELARLYPQLDRFTEICRQHDAGGVFRNRWLDSA
                     LFGHSNS"
     gene            1681..3096
                     /locus_tag="VFB80_11365"
     CDS             1681..3096
                     /locus_tag="VFB80_11365"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HZN34414.1"
                     /translation="MKTLGQLRRLCIVCLFASGLLPAAFGAETALDRYVKKPDPTYSW
                     KIAAEKQGDGVSQWVIDLKSQTWRTELDVDRPVWQHWLIVCKPEKATAKKALLFIVGG
                     ANGGEPPAAAEARTLAIAKATNAVVAELRMVPNQGLIFHGDGKSRKEDDLIAYTWDQY
                     LKTGDETWPARLPMVKSAVRAMDCVQELLASDKGGKLAIEKFVVAGGSKRGWTTWCTA
                     AVDPRVEAAIPIVIDVLNVQVSMRHHVAAYGAYSLAINDYYEHGLMQRSNDPRLADLY
                     AIEDPYSYRDRLTMPKFIVNSAGDEFFLPDSSQFYFGDLKGEKLLRYVPNSNHSLRDT
                     DAVESIIAFYQTVLAGTPRPKFGWTFEKDGSIRVQAETKPKAVRLWRATNPERRDFRL
                     AVIGKAYGSDTLSEQAGGTYVAQLAAPTKGWTASFVELEFDVGAAFPLKLTTAVRVLP
                     DRLPFADLDPAKATVEPRPAR"
     gene            complement(3093..>3302)
                     /locus_tag="VFB80_11370"
     CDS             complement(3093..>3302)
                     /locus_tag="VFB80_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34415.1"
                     /translation="TGAGELYRPERLPAQQRAAIQNWKERQAKGTIAYKLPPTPLENY
                     KPLANPAATEIPVPGRGVPAAPLAP"
BASE COUNT          534 a         1130 c         1072 g          566 t
ORIGIN      
        1 ggcgtgcact ggatccgctt gaacgtgcag ccgacggagc gggccagggc ccgggccagc
       61 atcgtcttgg cgacgccggg gacgtcctca aggaggatgt gtccctcgca cagccaggcc
      121 gtgagcgaga ggatcagctg cggccgcttg ccgacgatcg cccgttcgac attcgcaatg
      181 atccgcttgg cgacgccgcc gacatctgcc atatattcct cagcgtgcca ttctaatcca
      241 gacctcgctt catgccgatc cgcaacttcg gccgcaatct cgagttctcg ccccggcagg
      301 ggcatacgcc ggccagcgag caggaactgc tggaaatcct gcggcaatat cgcggccggc
      361 agatccgggc cgtcggccgg ctgcactcct ggagccaggc catcgtcgcc gacgatgtgc
      421 tgctcgacct gcggcagctc gacgccgtcc gcgtcgagca gcgcggcggc gaaacctggg
      481 ccgtcgtcgg cgcgggttgt cagatcaagc gcgtgctgag cgagctcgat cggcacggcg
      541 tgacgctgcc cacgctgggc ctgatctcgg agcagaccat cgccggggcc gccgccaccg
      601 gaacgcacgg ctccggccgg cacagcctct cgcactatct ggacgaggtg cggattgccg
      661 cgtacgacgc cgccggcgag ccggtcatcc gcacgatcac aggcggcgac gagctgcggg
      721 ccgcccgctg ctcgctcggc tgtctgggca tcgtcgtcag cgtcgcgttc cgctgccggc
      781 tgaagtacca catcgaagag cactttcgcg agtacgacag cctggacgac gtgctcgcgg
      841 ccgaagccga atatccactc acgcagttct acctggtccc ctggcgctgg aagtggatgg
      901 cccagcatcg ccgcgaatcg gccgcgccgc ggagttggct cgcgccgctc taccgctggt
      961 actggttcct gtggatcgac gtgatgctgc acctgaatgt tctgctgctg gtgaggtggc
     1021 tgagaatcgc cggcttgatc aagctcttct atcgctggat cgtgccgctg gcgatcgtcc
     1081 gcccctggcg cgtcgtcgat cgttcgcagg acatgctcct catggagcac gagttgttcc
     1141 ggcacctgga gctggaggtc ttcgttcgtc gctctcagct ggcggctgcc actgactttg
     1201 tgaggcagac gctgcaattc ttcggcggcg aagccgtgcc ccctctccca cagggagagg
     1261 ggacggccgg cagctacgtg catcactacg cgatctgcat ccgccaggtc ctgcccgacg
     1321 acgcgctgat ctcgatggcg agtggcggcg aggagccgca ctacgcgatc agcctgatca
     1381 cctacgcccg gcccagcgag cgggccggct tcctggcctg cgccgacttt ttggcccacc
     1441 gcatggccgc gctgttcgac gcccggccgc actggggcaa atactgcccg ctggacgcag
     1501 ccgagctggc ccggctttac ccgcagctgg atcgattcac cgaaatctgt cggcagcacg
     1561 acgccggcgg cgtctttcgc aatcgctggc tcgactcggc gctgtttgga cactccaact
     1621 cctagcggct atactcgcca cacttgtcct gtctcgttcg gctaatctcg ggagcggtcc
     1681 atgaaaaccc tcggtcagtt gcgtcggctg tgcatcgtct gcctgttcgc atcgggtctc
     1741 ctgccggccg ccttcggggc agaaaccgcc ctggaccgct acgtgaagaa gccggacccg
     1801 acctattcct ggaagatcgc ggccgagaaa cagggagatg gcgtcagcca gtgggtcatt
     1861 gacctgaagt cgcagacctg gaggacggaa ctggacgtcg atcgcccggt ctggcagcac
     1921 tggctgatcg tttgcaaacc cgaaaaggca accgccaaga aggccctgct ctttatcgtc
     1981 ggcggcgcga acggtggcga gcctcctgcc gcggccgaag cccgcacgct ggccatcgcc
     2041 aaagcgacga acgccgtcgt ggccgagctg cggatggtcc ccaatcaggg gctcatcttt
     2101 catggcgacg ggaagtcgcg gaaggaagac gacctgatcg cctacacctg ggaccagtac
     2161 ctgaagaccg gcgacgaaac gtggcccgcc cgcctgccga tggtcaagag cgccgtgcgg
     2221 gcgatggact gcgtgcagga gctcttggcc agcgacaagg ggggcaagct ggcgatcgag
     2281 aagttcgtcg tggccggcgg ctccaagcgc ggctggacga cctggtgcac ggccgccgtc
     2341 gatccgcgtg tcgaggccgc gattcccatc gtcatcgacg tgctcaacgt gcaggtctcg
     2401 atgcggcacc acgtcgccgc ctacggcgcc tattcgctgg cgatcaacga ctactacgag
     2461 cacggcctca tgcagcgctc caacgacccg cggctggccg acctgtatgc gatcgaagac
     2521 ccgtactcct atcgcgaccg gctgacgatg cccaagttca tcgtcaattc ggccggcgac
     2581 gagttcttcc tgcccgattc gtcgcagttt tactttggcg atctgaaggg ggagaagctg
     2641 ctccgctatg tgcccaactc gaatcactcg ctccgcgaca ccgacgccgt cgagagcatc
     2701 atcgccttct atcaaacggt cctcgccggc acgccgcgcc cgaagttcgg ctggacgttc
     2761 gagaaggacg gctccatccg cgtgcaagcc gaaacgaagc ccaaggcggt gaggctgtgg
     2821 cgggcaacga accccgagcg gcgcgacttc cgcctggccg tgataggtaa ggcttatggc
     2881 agcgacacgc tctccgagca ggccggcggc acgtacgtcg cgcagctggc ggcgccgacc
     2941 aagggctgga ccgcgtcgtt cgtcgagctg gagttcgacg tcggcgcggc gtttccgctc
     3001 aagctcacca ccgcggtccg cgtgctgccc gacaggcttc ccttcgccga cctcgacccc
     3061 gccaaggcca ccgtcgaacc ccgccccgcg cgctaaggcg ccagcggcgc cgccgggacg
     3121 ccgcggcctg ggaccggaat ttcggtcgcc gcgggattgg ccagcggctt gtagttctcc
     3181 agcggtgtgg gcggcagctt gtaggcgatg gtgcccttgg cctgccgctc cttccagttc
     3241 tggatcgcgg cccgctgctg ggcaggcagg cgctcgggcc gatacaactc gcccgcgccg
     3301 gt
//
LOCUS       DASRJB010000434         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1248197, whole genome shotgun sequence.
ACCESSION   DASRJB010000434 DASRJB010000000
VERSION     DASRJB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248197"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1392)
                     /locus_tag="VFB80_11375"
     CDS             complement(202..1392)
                     /locus_tag="VFB80_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34416.1"
                     /translation="MPVARHFAVLAVAAGLVAGARAADPPAPPPKPEQVARLITDLGS
                     EEFTVREAATDTLTRIGLPAFAALEAAATHSDREVRYRSQRILGLIREHDLKRRLEAF
                     LSGKEAEGDYPLPGWTRFKKTYGDEGQTRSLFVEMQRAEPDLLRALEEGPRGAADAVS
                     QRVAQVQEALRFGGQPQFTPGQVVSLLFVASEDDVSLPAATMTLFYNLCYQQPFREAI
                     EGQSRGGVPRKMLASLIRRSDENSAYMAMQVAYQLNMPEGIDPALKILNGKGGVRVGT
                     YSAYALATVVRTGDAAHLPLVEKLLTDASQVTQIQQNNVITKLQLRDSALAAAILLTK
                     QELKDYFDLASKPAITEPRSILINPSLIGFKTDAEREAVFKKWEVYKARQPQQPAEKP
                     APSP"
     gene            complement(1467..1946)
                     /locus_tag="VFB80_11380"
     CDS             complement(1467..1946)
                     /locus_tag="VFB80_11380"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HZN34417.1"
                     /translation="MGREHAKTLFGIKEDGPLLKFLDELKVRPDMKKSGRVLETGVLW
                     DPLHRCLTEGELDPAGGDFPLNHAVLGGKQLHKGADHAAILVRPDMVRFISDALAEIS
                     EETMRQKFFGLPKAACCVAQDEKHFMELWLALQNLQVFFQAAADNLEAVVFTAKWRE"
     gene            complement(2026..>2601)
                     /locus_tag="VFB80_11385"
     CDS             complement(2026..>2601)
                     /locus_tag="VFB80_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZN34418.1"
                     /translation="APEDREYFAEKFAGTAAMLGIEVVEGGAERYDSVQKALARVNAD
                     IDFVAVHDAARPCVAAEWIDAVFAAAAKSGAAILAIPVQGTVKRANPDQNIAETVARD
                     KLWEAQTPQVFRRQLLIDAYARRGQQPATDDAQLVERLGQPVTIVPGSPMNIKITTKE
                     DLRMASALLKALPRPKLAGPAHPFADDELWR"
BASE COUNT          412 a          832 c          877 g          480 t
ORIGIN      
        1 caatggacct cacggcgacg agaattgtaa ctatccgtcc agtatatgtc aagcgcaaca
       61 caacagattg tgttccgcct ccgcccgacc acaacctgta gtgggtcgcg ccttttagcg
      121 gcccctgaaa ggagggcggc ccctggccgc atcgccgcgg tccgggagcc gctggcgacg
      181 accgatccgg cgtgaaggag gctacgggga aggcgcgggc ttctcggcag gctgctgcgg
      241 ctggcgggcc ttgtagactt cccacttctt gaagaccgcc tcccgctcag cgtcggtctt
      301 gaagccgatc agcgaggggt tgatcaggat actgcgtggt tcggtgatcg ccggcttgct
      361 cgccagatcg aaataatcct tgagctcctg cttcgtcagc aggatcgcag ccgccagggc
      421 actgtcgcgg agctggagct tcgtgatgac gttgttctgc tggatctggg tgacctgcga
      481 ggcgtcggtg agcagcttct cgaccagcgg caggtgcgca gcatccccgg tccgcacgac
      541 ggttgccagg gcatacgccg aataggtgcc gacgcggacg cccccctttc cgttcaagat
      601 tttcagcgcc gggtcgatcc cctcgggcat gttgagctga taggccacct gcatcgccat
      661 gtaggcgctg ttctcgtcgc tgcgtcggat cagcgaggcc agcatcttcc gcggtacgcc
      721 gccacgcgac tggccttcga tcgcctccct gaacggctgc tggtagcaga ggttgtaaaa
      781 cagggtcatc gtggcggcag gcagcgaaac gtcgtcttcg gaggcgacga acaagagcga
      841 gacgacctgc ccgggcgtga actgcggctg gccgccgaac cgcaaggcct cctgcacctg
      901 ggccacgcgc tgactgaccg cgtcggccgc gccccgcggg ccttcttcca gggctcgcaa
      961 gaggtccggc tcggcccgct gcatttcgac gaacagcgag cgggtttgcc cctcgtcgcc
     1021 ataggtcttt ttgaagcgcg tccatcccgg cagcggatag tcgccctcgg cctccttgcc
     1081 gctgaggaag gcttccagcc tccgcttcag gtcgtgttcg cggatcaggc ccagaattcg
     1141 ctggctgcgg tagcggactt cgcggtccga atgcgtcgcg gcagcctcca gggcggcaaa
     1201 cgccggcagg ccaatgcggg tgagcgtgtc ggtcgccgct tcgcggaccg taaactcttc
     1261 gctgcccagg tcggtgatca gccgggcgac ctgctccggc ttcggcggcg gcgccggcgg
     1321 atcggccgct cgcgctccgg ctacgaggcc agccgcgact gccaggactg cgaaatgtcg
     1381 tgcgacaggc atggcgtgga cctcagcagg cgggaatggc cctcagggac gattgtaggt
     1441 cgcaccgcca cgattcgtcc agcgcgctat tcccgccatt tggccgtgaa gacgacggct
     1501 tccaggttgt cggccgcggc ctggaaaaag acctggaggt tttgcagggc gagccagagc
     1561 tccatgaagt gcttttcgtc ctgggcgacg cagcacgcgg ccttgggcag gccgaagaat
     1621 ttctgccgca tggtttcttc gctgatttcg gccagtgcgt cggagataaa gcggaccatg
     1681 tcggggcgga cgagaatcgc ggcgtggtcg gcgcccttgt gcagctgctt gccgccgagc
     1741 acggcgtggt tgagcgggaa atcgccgccg gccgggtcga gctccccttc ggtcaggcag
     1801 cggtggagtg ggtcccagag gacgcccgtc tccaacacgc ggccgctctt cttcatatcg
     1861 ggccggactt tgagctcgtc gagaaacttg agaagcgggc cgtcttcctt gatgccgaac
     1921 aaagtcttgg cgtgttcgcg acccaaagcg atgtgaaatc ctcggccagc caccggagca
     1981 cctgattcct tgttggatag agtcggacgt ttctcagcaa tcacctcacc gccacagctc
     2041 gtcgtcggca aacggatgcg cgggtccagc cagcttcggc cggggcagcg ccttgagcag
     2101 cgcggaggcc atccgcaaat cctctttcgt cgtgatcttg atgttcatcg gcgagccggg
     2161 aacgatggtg accggctggc ccagccgctc gaccagctgc gcgtcgtcgg tcgcgggctg
     2221 ttgtccgcgg cgggcgtagg cgtcgatcaa gagctgccgg cgaaagacct gcggcgtctg
     2281 ggcttcccag agcttgtcgc gggcgacggt ctcggcgatg ttctggtcgg gattggcccg
     2341 cttgacggtg ccctggacgg gaatggccag gatcgccgcg ccgctcttgg ccgcggcggc
     2401 gaatacggcg tcgatccact cggcagcgac gcaggggcgg gcggcgtcgt gcacggcgac
     2461 gaagtcgatg tcggcattga cccgggccag ggctttttgc accgagtcgt accgctccgc
     2521 gcccccctcg accacttcga tcccgagcat cgcggcggtg ccggcaaact tttcagcaaa
     2581 gtactcgcgg tcctcgggag c
//
LOCUS       DASRJB010000435         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3503203, whole genome shotgun sequence.
ACCESSION   DASRJB010000435 DASRJB010000000
VERSION     DASRJB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503203"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFB80_11390"
     CDS             complement(<1..604)
                     /locus_tag="VFB80_11390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN34419.1"
                     /translation="MRYLLAVLFAVAILSPVRAADPAGFHTLAIGEAAPDFKLPGVDD
                     KEYSLASFAGAKLLCVVFTCNHCPTAQAYEERIAKLQADYKDKGLALVAISPNDPLAV
                     RLDELGYTDVSDSFAEMKLRARAAKFAFPYLYDGETQRTSLAYGVVATPQVFLFDQDR
                     KLRYVGRIDDSDVKTVTSHDAKNAIDALLAGKPVPVEKTRT"
     gene            complement(804..2072)
                     /locus_tag="VFB80_11395"
     CDS             complement(804..2072)
                     /locus_tag="VFB80_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34420.1"
                     /translation="MLKIRFGSTTGYCDGVSRRSFLKLGAMTIGGLTLADLLRAEALA
                     GVGSSNKAIINVHLSGGPSHQDMWDLKPDAPAEFRGEFNPIATNVPGMEICEHMPRLA
                     TLADKFAVIRSIVGSAGEHSNHQTHTAYHTRDLQNAGGRPSLGSVVNKLFGEGQNGAP
                     AFISYNGGEPGYLGPVYKPFQPMGGSLALNNNLNAQRLDDRTNLLSSLDKIRRDVDKS
                     GQMAALDSFTQRAVDVVLSGKVADALDTRKQDPKDAERYGRETQPFLTARRLVEAGVR
                     VVTFSWGGWDTHGGNFTTLKRQLPNLDRGLANLLEDFAASGRDKDVTIIVWGEFGRTP
                     RVNSGAGRDHWPQVMAAFLAGGGMRTGQAIGTTTKYAENAKDRPVQVQEIFATLYRNL
                     GIDARTTQLFDTAGRPQYLLEKRDALPELA"
     gene            complement(2375..3064)
                     /locus_tag="VFB80_11400"
     CDS             complement(2375..3064)
                     /locus_tag="VFB80_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34421.1"
                     /translation="MVAAPGLPRWLSLLAALSLSGLLGCAPGNVWVYIDNAGDEPLVV
                     KVDGKEAAKIPPGEFAELNYPPGEYQFLITAGGQTVCDLKKNLEKSDTFGRTRKYLFN
                     PDKNNCYGSYEVKYGGSRLDGVMEASLLSHQKDPKIRAKYAYNKLLKEIDLVSTDAWN
                     DVSGVEYILSPPPETMMTRSGSSRRVTVLDRLDKDDYRRLKEMSRRDNPDDADVDELE
                     ELIDEILSKAL"
     gene            3259..>4762
                     /locus_tag="VFB80_11405"
     CDS             3259..>4762
                     /locus_tag="VFB80_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34422.1"
                     /translation="MAKESWAKKPPARPGRGARWLSRAVWTLALLALLAWFGWLLIRP
                     FWHPRTHLVLLTGDVVSVDASPAAIPADYVVEDFRELLKLESVLHQGLLDEPAQPLVL
                     GSLSSPDQMQQLADLLNTRVTGRGDVILIYVSAHGLTQDGEAWLLASGTDPQAPLGGR
                     YRLTSLLAQLRECEARAKVLLLEAGRIDFDPLRGLVDNDFPCRLAEQVRELGDPSLWV
                     LVSHGAGQRSHLSPGLRQSVFGHYVASGLAGAADANADREIDLAELNDFVTRGVSGWI
                     RQTTGGASQQTPVLLWGGGDVPTRGLPTLAAVRKGPAPKSADSAAIANGQPGQPAAEG
                     QPPGAAAEIQTRTQQEIDSLISRSVMRRAPQGQVGQYANETVQKWLAQARGGAAQLTG
                     GGEDKEEQKNLGGEASPQPPDAKTETKKSDEGDKGKAEQSQPAEDRAKAKENEKKAKS
                     EPAVEKPKEGKEKAEPPPAEPQPTAPPPPPPVTPDERAAIAAQLQRGWQLR"
BASE COUNT          839 a         1490 c         1636 g          797 t
ORIGIN      
        1 aggtgcgggt cttctcgacg ggcacgggct tgcctgccag gagcgcgtcg atggcgttct
       61 tggcgtcgtg gctggtgacg gtcttcacgt cggagtcgtc gatgcggccg acgtaacgga
      121 gcttgcggtc ctgatcgaac aggaagacct gcggcgtggc gacgacgccg taggccaggc
      181 tcgtccgttg cgtctcgccg tcgtagagat acggaaaggc aaacttcgcc gctcgggcgc
      241 gcagcttcat ctcggcgaag gaatcgctga cgtccgtgta gcccagctca tccagccgca
      301 cggccagcgg atcgttgggc gaaatggcga ccagggccag gcccttgtcc ttgtagtcgg
      361 cttgcagctt cgcgatccgc tcttcgtagg cctgtgcggt tgggcagtga ttgcaggtga
      421 agacgacgca cagcagcttc gccccggcga agctggccag cgagtactcc ttgtcgtcca
      481 ctcccggcag cttgaaatcc ggcgcggcct cgccgatcgc cagcgtatgg aagccggcag
      541 gatcggcggc tcggaccgga gaaaggatag ctacggcgaa cagcacggcc agcaagtatc
      601 gcatcgtcgg tgctccggac gggaatgcgg cgactgtccg ggaattttag atcgtccgcg
      661 ggcaagttac gctagggcgc ggcgaaaaat cctgagccgc gaaattgccg cgcaggggcg
      721 cgctggcagc gtgccctacg ggaacgccac aaaaggcgga aacgccacgg agggggaacg
      781 ccacggaggg cgttcactac aggtcaggcg agctcgggga gggcgtctcg cttttccaaa
      841 aggtactgcg ggcggccggc ggtgtcgaag agctgagtgg tgcgggcatc gatgccgagg
      901 ttgcgataga gcgtggcaaa gatctcctgc acctgcaccg ggcggtcctt ggcgttttcc
      961 gcgtacttcg tggtagtgcc gatggcctgg ccggtccgca tgccgccgcc ggccaggaac
     1021 gccgccatca cctgcggcca gtggtcgcgg cccgcaccgc tattcacccg cggcgtgcgg
     1081 ccgaactcgc cccacacaat gatcgtgacg tccttgtcac ggccgctggc ggcaaaatcc
     1141 tcgagcaggt tcgccaggcc gcggtcgagg ttcggcagct gccgcttgag cgtcgtgaag
     1201 ttgccgccgt gggtgtccca gccgccccaa ctgaacgtca ccacgcggac gcccgcctcg
     1261 accagccggc gagcggtcaa aaacggctgc gtctcgcggc cgtagcgctc ggcgtccttc
     1321 gggtcctgct tccgagtgtc cagcgcgtcg gccaccttgc cgctcagcac gacatcgacg
     1381 gcccgctgcg tgaagctgtc gagggccgcc atctgcccgc tcttatcgac gtcgcggcga
     1441 atcttgtcga gcgagctgag caggttcgtg cggtcatcga gccgctgggc gttgagattg
     1501 ttgttgaggg ccagcgagcc gcccatcggc tggaagggct tataaaccgg gccgagatag
     1561 ccgggctcgc cgccgttgta ggaaatgaag gccggagcgc cgttttgacc ttctccaaat
     1621 agcttgttca ccacgctgcc cagcgagggg cggccgccgg cgttctgcag gtcgcgagtg
     1681 tgataggcgg tgtgggtctg atgattggag tgctcgccgg ccgagccgac gatcgagcgg
     1741 atgacggcga acttatcggc cagcgtggcc agccgcggca tgtgctcgca gatttccatg
     1801 cccggaacgt tcgtggcaat cgggttgaac tcgccgcgaa attcggcggg ggcgtcgggc
     1861 ttgaggtccc acatgtcctg atggctgggg ccgccgctca ggtggacgtt gatgatcgcc
     1921 ttgttcgagc tgccgacacc cgccagggcc tcggcccgca agaggtcggc cagcgtcagg
     1981 ccgccgatcg tcatcgcccc cagcttgagg aacgaacgcc gggaaacgcc gtcgcagtag
     2041 ccggtcgtgg agccaaagcg aatcttgagc atggctgatt ccttctgttc caggtgggac
     2101 gaaataggcg ggctatctct cgaatggccg gggcaggcaa acggttacac aaattgggtc
     2161 aaaacgcggc cttgccgccc ttgccgattc ccttcgtacc gcaatcggcg cgacaaatca
     2221 agattcttgg atggatttag gtcgctagcg gcagatcagc gcaaagcgga atcttgagaa
     2281 aagctcaggc gacgccccag agacgggcgt cctcagaaag ccctcgctga cgcgttgggt
     2341 tggcgtaccg gcggccgcga cgccctggtc acacctacag cgctttgctc agaatctcgt
     2401 cgatcagctc ttccaactcg tcgacgtcgg catcgtcggg attgtcccgg cgggacattt
     2461 ccttcagccg gcggtagtcg tccttgtcga ggcggtcgag gacggtgact cgccgcgagc
     2521 tgccgctgcg agtcatcatc gtttcaggcg gcggcgaaag gatgtattcc acccccgaga
     2581 cgtcgttcca ggcatcggtc gaaacgaggt cgatctcttt gagcagcttg ttgtaggcgt
     2641 atttcgcccg gattttcggg tccttttgat ggcttagcag gctcgcttcc atcacgccat
     2701 ccaaccggct gccgccgtac ttcacctcgt aagagccgta gcagttgttc ttgtccggat
     2761 tgaagaggta cttgcgggtc cggccgaacg tgtcggactt ctccagattc tttttcaggt
     2821 cgcagacggt ctggccgccc gcggtgatga ggaactggta ctcgcccggc ggatagttca
     2881 gctccgcgaa ctcgcccggg ggaatcttgg ccgcttcctt gccgtccact tttacgacca
     2941 gcggctcgtc gccggcgttg tcgatataga cccagacgtt ccccggcgca cagcccagga
     3001 ggcccgatag cgacagggcc gcgagcaagc tcagccaacg gggcaatcca ggagcagcga
     3061 ccatcgggca cctcgcaatc gtagaactcg tggaatgagc aaccgtagga tgcgcaatcg
     3121 caccgggcaa atggccggtt ggcgtcccgc tgaacggcgt caccagcaga caattcggaa
     3181 ttgcccgcgc gatcggatgt aggctataca actcgttggc agactgggat ttagggcgaa
     3241 agcagaggca aatttcgcat ggccaaggaa tcgtgggcga agaagccgcc ggctcgcccg
     3301 ggccgggggg cccgctggct gtcgcgcgcc gtctggacgc tcgccctgct ggccctgctg
     3361 gcctggttcg gctggctgct gatccgcccg ttttggcacc cgcggacgca cctggtgctg
     3421 ctcaccggcg acgtggtctc ggtcgatgcc tcgccggcgg cgattcccgc ggattatgtg
     3481 gtcgaggatt ttcgcgagct gctgaagctc gaaagcgtgc tgcaccaggg actgctcgat
     3541 gagccggcgc agccgctggt gctcggcagc ctgagcagcc cggaccagat gcaacagctg
     3601 gccgacctgc tcaatacgcg ggtgacgggc cgcggcgacg taatcttgat ctacgtctcg
     3661 gcgcacgggc tgacgcagga cggcgaggcg tggctcctgg ccagcggaac cgatccgcag
     3721 gcgccgttgg ggggccgcta tcggctgacg agcctgctgg cccagctgcg cgaatgcgag
     3781 gcgcgggcga aggtcctgct gctggaagcc ggccgcatcg atttcgatcc actccgcggc
     3841 ctggtggaca acgattttcc ctgccggttg gcggagcagg tccgcgagct gggcgatccg
     3901 tcgctgtggg tgctggtctc gcacggggcc gggcagcgct cgcacctctc gccggggctg
     3961 aggcagagcg tcttcgggca ttacgttgcg tcgggcctgg ccggagcggc cgacgccaac
     4021 gccgatcgcg agatcgacct cgccgagctg aacgactttg tcacccgggg cgtctccggc
     4081 tggattcgcc agacgaccgg cggagcctcg cagcagacgc ccgtgctgct ctggggcggc
     4141 ggcgatgttc cgacgcgcgg gctgcctaca ctggcggctg tacgcaaggg ccccgcgccg
     4201 aaatcggccg actcggcagc aattgcaaat ggccagcccg gtcagccggc ggccgaaggt
     4261 cagccgcccg gcgcggccgc ggaaattcag acccggacgc agcaggaaat cgactcgctg
     4321 atttcccgca gcgtgatgcg cagggcgccg cagggacagg tcgggcagta cgccaacgag
     4381 accgtgcaga aatggctcgc ccaggcccgc ggaggtgcgg cccaactgac cggcggcgga
     4441 gaagacaagg aagagcagaa gaacttgggc ggcgaggcct ccccgcaacc gcccgacgcg
     4501 aagacggaga cgaaaaaatc ggacgagggc gacaaaggca aggcggagca aagccagccg
     4561 gcagaggatc gggccaaggc gaaggaaaac gagaaaaagg cgaagtccga gccggcagtc
     4621 gagaagccaa aggaaggcaa agaaaaggcc gaaccgccgc ccgccgaacc gcagccaaca
     4681 gcgccgccgc caccaccgcc ggtgacgccc gacgagcggg ccgccattgc cgcgcagctg
     4741 cagcggggct ggcaattgcg cg
//
LOCUS       DASRJB010000436         9474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3786594, whole genome shotgun sequence.
ACCESSION   DASRJB010000436 DASRJB010000000
VERSION     DASRJB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786594"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..3578
                     /locus_tag="VFB80_11410"
     CDS             402..3578
                     /locus_tag="VFB80_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN34423.1"
                     /translation="MVLSIFLVFGAAAAGQEGKWFTQQPEVMPTVPAGRPAADGALAY
                     GPSARGPWRRGPAANWIWGRKDLDRYYLRTSFAVPAGLKAARLRITCDNRFVALLNGK
                     EIARGDDWNSPVEVDFTAAAKAGQNELLVEATNEGSAAGLIAKILLTKADDTIEYVVT
                     DDTWQAAEAKDAQTWEKARRIAAAGGGPWGDVFNRAAGGAGSASPSFQLLPGFQVERL
                     FTVPKNELGSWVAITTDNKGRLIASDQERQGLCRITPPPIGSSEPTKVERLDVKITAA
                     QGLLYAFDALYVSVNGGPGSGLYRCKDTNGDDQFDEVVKLKDIRGGGEHGPHALRLAP
                     DGKSIFLCAGNHTQPPFDVQRNSPPQTMGGVRQTILQAELPTGARSLLAPNWDEDLLL
                     PRQWDSNGHAAGILAPGGWVAKTDPDGKTWEMFTVGYRNHYDFAFNADGEMFCYDSDM
                     EWDMGTPWYVPTRVVHATSASEFGWRSGTGRWPAYYVDSLPPLVDVGPGSPVGVEFGY
                     GTKFPAKYQRALYICDWTFATMYAIHMEPAGASYKATKEEFVTRTPLPLTDAAVGKDG
                     ALYFTIGGRGAQSELFRVTYVGSESTAPVDGKDTAGADLRELRREIERFHDPAFGADR
                     PAESNDYLVSHLGHPDRHIRYAARIALERRSGWSDKALAATDANAVIEAAVGLARSAR
                     SKDAQQRLLAALGRLDFAKLAAQQQLDLLRAYQLALIRLGDVDDATRQQLAVRFAALF
                     PQPGDEANRELAILLVYLQAPDAAKKIVPLLSKQHAPVTETPTEVLARNQGYGRSIAS
                     MLANQPDLQQIHYAFTIRNLKAGWTPDLRKDYFQWFTKAHGWSGGNSFQKFLTNIDSD
                     AFANCTDVERLTLEALGARQPYVPPPLPKAAGPGHAWTIEEVIKLGSEQLSGRSFRQG
                     QRAFAAARCIVCHRFAGDGGATGPDLTQLAGRFNLKDLTDALLDPSKVISDQYKASVI
                     RKQNGEVITGRIVSDLPDAITVLVNPEDASKTVKIAKSEIEQQAPSATSLMPKDLLSP
                     LNEQEVLDLLAYLLSRGNPQDPMFRRGN"
     gene            3746..6124
                     /locus_tag="VFB80_11415"
     CDS             3746..6124
                     /locus_tag="VFB80_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34424.1"
                     /translation="MSAQEKPLDFAHEVVPILRKHCGKCHTGEKKEGAFSMNTRASLV
                     AGGETGPAVVSGKSAASDLIKRITSSDDTIKMPPEGPRVAATEVDLLKRWIDGGLAWE
                     EGFAFRASSYEPPLAPRRPMLPPSSGSRKNLIDLILGNWLAERKLPRPQPISDEQFLR
                     RVSLDLVGLLPTPEQREKFLADSRPDKRALLVKELLADDVAYAEHWLTFWNDLLRNDY
                     TGTGFITGGRKQITKWLYEALVTNKPYDQFARDLIAPPTPESAGFIEGIKWRGEVSAG
                     QTVEIQFAQSIGQSLLGINLKCASCHDSFIDRWKLSESYGLAAIYSTRPLPIHRCDKP
                     TGESAQAGWLFPELGKIDAAAPQPERLKQLAALMTHPENGRFTRTIVNRLWHRLMGRG
                     IVHPVDAMQTEPWNADLLDYLAVHLADNGYNLKATLQLIATSDAYQSRSQIVTKELAA
                     GEYVFAGPRARRLTAEQFLDAVWQLTGAGPTRYDAPVLRGKPDPAAGEQQKLTGKWIW
                     NKADAGAAPAGESVAFRKQFDLPQAPQQALAVISCDNSYTLFVNGQKAQAGDNWESPD
                     AAVLTERLKAGANDLLVVAKNAGNSPNPAGLFCEVRIRLADGTDVTHGTDASWEWTAK
                     LPNGNGRFVREQPTDWQPAAIALKSEVWSAKLDGPLAALLARGAIGAELMVRASLVKS
                     DFLMRTLGRPNRDQIVTVRPAELTTLEAIDLANGEILAGTLQRGAQKLLAREWQSPEH
                     FVRWLYQFALCREPAAEELAILKESLGEKLTAQGIEDALWTVIMLPEFQLVR"
     gene            6127..6519
                     /locus_tag="VFB80_11420"
     CDS             6127..6519
                     /locus_tag="VFB80_11420"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN34425.1"
                     /translation="MPKPSKPKDIDAYFAQFPAGVQRILKKVRQTIRRAAPDAEEVIS
                     YQMPAFRQHGILVYFAAWKDHIGLYPPISGDAALERAVARYAGPKGNLQFPLDEPIPY
                     HLIARLVKLRVKQDAAKANAKRKGRKSG"
     gene            6608..8059
                     /locus_tag="VFB80_11425"
     CDS             6608..8059
                     /locus_tag="VFB80_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34426.1"
                     /translation="MIHDNPNPQARRRFLQQVSAAGAAALLAGEPRLLLAAPGGEKLV
                     HPAAKADAVIVLWMGGGMAAPDTFDPKKYVPFQVGLPVGDVESCFPAIDTVVDHIKIT
                     QGLEQIAQVMDRATLIRSHVLPDLGSILHSRHQYHWHTGYVPPQTVACPHIGAWLARV
                     KGPQNPVIPPFINIGQRLEGVGEQEELKAFTTAGFFGSEFGPMNLPYPEEAAQSVRPP
                     KGMEGDRFSSRYRLYQKLLRESPDRDARSDYQRESMLRALDNAHRLLSAPERTAFDIS
                     LEPKESYEKYDTGRFGRGCLLARRLIEAGARYIEVTTEYVPFLNWDTHANGYTTLVEM
                     KKQIDRPIAQLIRDLEARQLLERTLVILASEFSRDMLIEGRPGSTANDQATEKVDRFQ
                     ALKHYGQHRHFTGGSCVVMWGGGVKRGFLYGETAKERPFLAIKNPLTVTDLHATIFTA
                     LGVSPKTAFDVEKRPFYATEDGKGQPALELFGA"
     gene            complement(8063..8602)
                     /locus_tag="VFB80_11430"
     CDS             complement(8063..8602)
                     /locus_tag="VFB80_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZN34427.1"
                     /translation="MDIFRLPQVILLAQESSGSRGFLGTRGSLMLDVVFLAMFVIVPC
                     LAASIWLVKQRRQYLLHKRLQLAMAAVLLTAVGLFEIDLQLFTNWEDRAAASPYFDAA
                     HKWSCPVGISLIVHLCFAVPTFCLWILVVVQALRKFSRPPQPGPHSRWHARAGWLAAA
                     GMTLTAVTGWFFYWLAFVA"
     gene            8699..8785
                     /locus_tag="VFB80_11435"
     tRNA            8699..8785
                     /locus_tag="VFB80_11435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8733..8735,aa:Leu,seq:taa)
     gene            8883..9077
                     /locus_tag="VFB80_11440"
     CDS             8883..9077
                     /locus_tag="VFB80_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34428.1"
                     /translation="MLSFLPEGRLASWGGAGGSLVIADVDRRLTFAYVMNLMRPALVG
                     PVAEALVKRLYDIVSHPAGS"
     gene            complement(9085..>9474)
                     /locus_tag="VFB80_11445"
     CDS             complement(9085..>9474)
                     /locus_tag="VFB80_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34429.1"
                     /translation="DPRKASRQALFADLEAMNYVSFAESKDDKDLLLVTVDMPYPAEL
                     GSLKLESVGLGWDKFHRNDLSSDQATKSESPASAKQLPSYGQIRDVVSKHGASLKGIR
                     WSEQHACRPLGCVTAPATRTAQSQAQR"
BASE COUNT         1774 a         3130 c         3064 g         1506 t
ORIGIN      
        1 gtcgccagcc ggccagcgcc aaattggcag gtccgggcgc gatcggaacc tcagtgcaaa
       61 taccatacca atgttcacat ttccgccagc cagttgttgt ggccggtctc cgaccgagcc
      121 actgcaccgc ggatcgaacc gcaaggtctg caaggcgccg gagatctgcg gccgccacag
      181 tggcaattgg gccgcagatc tacacggatc agcatgccgc cgctccgatc cgcgtcatcc
      241 gtgcccatcc gcggctaata actgctgcgc gcgtaccaca actggggcca caactcgaat
      301 cgacggctgc aaggcgccta tttcgcgaat atgatgggcg ctttgcctgt ccatcgaatc
      361 tgctgaaagg cccgcgagtg atgccgttgc atgttcggtt cgtggttctg tccatattcc
      421 tcgtgtttgg tgcggcggcc gcgggccagg aggggaagtg gttcacgcag cagccggagg
      481 tgatgcccac ggttcccgcg gggcggccgg cggcggacgg cgcgttggcc tacggcccta
      541 gcgctcgcgg cccgtggcgg cgcggcccgg cggcgaactg gatttgggga agaaaggacc
      601 tggaccgcta ctacctgcgg acttcgttcg cggttcccgc gggactgaag gccgcccgcc
      661 tgcggattac gtgcgacaac cgcttcgtgg ccctgctcaa cggcaaggaa atcgcccgcg
      721 gcgacgactg gaactcgccc gtcgaggtcg atttcacggc tgccgccaag gcgggacaga
      781 acgagctgct cgtcgaggcc accaacgaag ggagcgccgc gggcctgatc gccaagatct
      841 tgctcacgaa ggccgacgac acgatcgagt acgtcgtcac cgacgacacc tggcaagccg
      901 ccgaggcgaa ggacgcccag acgtgggaga aggcccggcg aatcgccgcg gccggcggcg
      961 gaccgtgggg cgacgtgttc aacagggccg ccggcggcgc aggcagcgcg agcccgtcat
     1021 ttcaactcct gccgggtttt caggtcgagc ggctgtttac ggtgcccaag aacgaattgg
     1081 gctcgtgggt cgcgatcacg accgacaaca agggccggct gatcgccagc gaccaggagc
     1141 ggcagggcct gtgccggatc acaccgccgc cgatcggcag cagtgaaccg acgaaagtcg
     1201 agcggctgga cgtgaagatc acggcggccc agggcctctt gtatgcgttc gacgcgctct
     1261 acgtctccgt caacggcggg ccgggcagcg gcctatatcg ctgcaaggac accaacggcg
     1321 acgaccagtt cgacgaggtc gtgaagctga aggacatccg cggcggcggt gagcacgggc
     1381 cgcacgccct gcgcctcgcg cccgatggca agtcgatctt tctctgcgcc ggcaaccaca
     1441 cgcagccgcc gtttgatgtc cagcgcaaca gcccgccgca gacgatgggc ggcgtgcggc
     1501 agacaatcct ccaggccgaa ttgccgaccg gggccaggag cctgctcgcc cccaactggg
     1561 atgaagacct gctcctgccg cggcaatggg actccaatgg ccacgcggct ggaatcctcg
     1621 ctcccggcgg ctgggtcgcc aagaccgatc cggacggcaa gacctgggag atgttcacgg
     1681 tcggataccg caaccattac gacttcgcct tcaacgccga cggcgagatg ttttgctacg
     1741 actcggacat ggagtgggac atgggcacgc cctggtacgt gcccacgcga gtcgttcacg
     1801 ccaccagcgc cagcgagttc ggctggcgga gcggcaccgg ccgctggccc gcgtattacg
     1861 tcgacagcct gccgccgctg gtcgatgtcg gccccggctc gccggtcggc gtcgagttcg
     1921 gctacggcac aaaattcccc gcgaagtacc agcgggcctt gtatatctgc gactggacgt
     1981 tcgccacgat gtacgcgatc cacatggagc cggccggcgc gagctacaaa gcgacgaagg
     2041 aggaattcgt cacgcgcacg ccgctgccgc tgaccgacgc ggcggtcggc aaggatggcg
     2101 cgctctattt cacgatcggc ggccgcgggg cacagtcgga actgttccgc gtgacgtacg
     2161 tcggcagcga atcgaccgcg ccggtcgatg gaaaagacac ggccggcgcg gacctgcggg
     2221 agctccgccg cgagatcgag cgttttcacg atccggcgtt cggcgcggac cgaccggcgg
     2281 agtccaacga ctacctcgtc agccacctcg gccatccgga ccgccacatt cgctacgccg
     2341 cccgcatcgc cctggagcgc cgcagcggct ggagcgataa ggcactggcc gctacggacg
     2401 ccaacgcagt catcgaagca gccgtcggcc tggcccgctc ggctcgcagc aaggacgcgc
     2461 agcaaaggtt gctcgccgcg ctgggccgtc tcgatttcgc gaagctcgcc gcgcagcaac
     2521 aactcgacct gctgcgggcc tatcagctgg ccctcatccg cctgggcgac gtcgatgacg
     2581 ccacgcggca gcagctggcg gtgagattcg cggccctctt tccgcagccg ggcgacgagg
     2641 cgaatcgcga gctggccatc ctgctggtct atctgcaagc cccggacgcc gcgaagaaga
     2701 tcgtgccgct cttgtcaaag cagcacgcgc cggtcaccga aacgccgacc gaagtgctgg
     2761 cccgcaacca gggctacggc cgcagcatcg ccagcatgct cgccaaccag cccgacctgc
     2821 agcagattca ttacgccttc acgatccgca acctcaaggc cggctggacg cccgacctgc
     2881 ggaaggacta tttccagtgg ttcaccaagg cccacggctg gagcggcggc aactcgtttc
     2941 agaaattcct gaccaacatc gacagcgacg cctttgccaa ctgcaccgac gtcgagcggc
     3001 tgacactgga ggctctcggg gcgcggcagc cgtacgttcc gccgccgctg ccgaaggcgg
     3061 ccggccccgg gcatgcgtgg acgatcgaag aggtcatcaa gctgggcagc gagcagctat
     3121 ccggccgcag cttccgacaa ggccagcggg cgttcgccgc ggcgcggtgc atcgtctgcc
     3181 accgttttgc cggcgacggc ggcgcgaccg gccccgacct gacgcaattg gctgggcggt
     3241 tcaacttgaa ggatctgacc gacgcgctgc tcgatcccag caaagtcatc tccgaccagt
     3301 acaaggcctc ggtcatccgc aaacaaaacg gcgaggtgat caccggccga atcgtcagcg
     3361 atttgcccga tgcgatcacg gtgctggtca atccggaaga cgccagcaag acggtaaaga
     3421 tcgccaagag cgagatcgag cagcaagccc catcggcgac gagcttgatg cccaaagacc
     3481 tgctcagccc gctcaacgag caggaagtcc tcgacctgct ggcctacctg ctctcgcgcg
     3541 gcaacccgca ggacccaatg ttccgccggg gcaattagcg aatagcggca acagaaggaa
     3601 ttgaacagaa ggaaacgaag gcaaagaaga ggacgagagg accagaccga tcatggaaac
     3661 gccgattgca ccactgcctt ccttctcgct gcttcgttcc cttcgtttcc ttctgtttat
     3721 ctccctgctc gcttttgtat ttcccgtgtc ggcccaggag aagccgctgg attttgcgca
     3781 tgaggtggtt ccgattctgc ggaagcactg cggcaagtgc cacacgggcg agaagaagga
     3841 aggcgcgttt tcgatgaaca cccgcgcgtc gctggtggcg ggcggcgaga cggggccggc
     3901 ggtggtcagc ggcaagagtg cggccagcga cctgatcaaa cggatcacca gcagcgacga
     3961 caccatcaag atgccgccgg aaggtccgcg ggtcgccgcc acggaggtcg atctcctcaa
     4021 gcgctggatt gacggcggcc tggcctggga ggaaggtttt gcctttcgag cgagcagcta
     4081 tgagccgccg cttgcgccgc gacggccgat gttgccgcct tccagcggtt cgcgcaagaa
     4141 tctgatcgac ctcatcctcg gcaactggct ggccgagcgc aagctcccgc ggccccagcc
     4201 gatcagcgac gagcagttcc tccgccgggt ttcgctcgac ctggtgggcc tcttgccgac
     4261 gcccgagcag cgggagaaat tcctcgccga ttcgcggccg gacaagcggg cactcctcgt
     4321 gaaggaactc ttggcggatg acgtcgccta cgctgagcac tggctgactt tctggaacga
     4381 cctcttgcgg aacgactaca ccggcaccgg gttcattacc ggcggccgca agcagatcac
     4441 caagtggctc tatgaagccc tggtgaccaa caagccgtac gaccagttcg cccgcgacct
     4501 gatcgccccg cccacgcccg aatcggccgg cttcatcgaa gggatcaagt ggcgcggcga
     4561 ggtgagcgcc ggccagaccg tcgaaattca attcgcccag agcatcggcc agtcgctgct
     4621 gggcatcaat ctcaagtgcg cctcgtgcca cgacagcttc atcgaccgct ggaagctgag
     4681 cgagtcgtac gggctggccg cgatttactc cacacggccg ctgcccatcc accgctgcga
     4741 caagcccacc ggcgaatccg cccaggccgg ttggctcttt cccgagctcg gcaagatcga
     4801 cgcggcggcc ccgcagccgg agcgcctgaa gcagctcgcg gctctgatga cgcacccgga
     4861 aaacggccgc ttcacgcgga cgatcgtcaa ccgtctctgg caccgcctga tgggccgcgg
     4921 catcgtgcat ccggtcgatg cgatgcagac cgagccctgg aacgccgacc tgctcgacta
     4981 tctcgcggtg cacctggccg acaacggtta caacttgaag gcgacgctgc agctgatcgc
     5041 tacttctgac gcttatcaga gccgctcgca gatcgtgacg aaggagctcg cggccggcga
     5101 atacgtcttt gccggcccgc gggcccggcg tttgaccgcc gagcagttcc tggacgccgt
     5161 gtggcagctc accggcgcgg ggccgacgcg gtacgacgcg ccggtccttc gcggcaagcc
     5221 tgatcccgct gccggcgagc agcagaagct gacgggcaag tggatctgga acaaggccga
     5281 cgctggggcc gcgcccgcgg gcgaatcggt cgccttccgc aagcaattcg acttgccgca
     5341 agcaccgcag caggcgctgg cggtcatctc ctgcgacaac agctacacgc tgttcgtcaa
     5401 cggccagaag gcgcaggccg gcgacaactg ggaatcgccc gacgcggccg tgctcaccga
     5461 gcggctgaag gccggcgcta acgacctgct tgtcgtggcc aagaacgcgg ggaattcccc
     5521 gaatcccgcc gggctgtttt gcgaagtacg gatccggcta gccgatggga cggatgtgac
     5581 gcatgggacc gatgcgtctt gggagtggac ggcgaaacta cccaacggca acggccgctt
     5641 tgttcgcgag cagccgaccg attggcagcc ggcggcgatc gccctgaagt ccgaagtctg
     5701 gagcgcgaag ctcgatggcc cgctggcggc gctgctggcc cgcggggcca tcggcgcgga
     5761 gctgatggtg cgggcctcgc tcgtgaagag cgacttcctg atgcgaaccc tcggccgccc
     5821 caaccgcgac cagatcgtca ccgtccgccc ggccgagctg acgaccctgg aagccatcga
     5881 tctggccaac ggcgaaatcc tggccggcac cctgcagcgc ggcgcccaga agctgctggc
     5941 ccgcgaatgg caatcgcccg agcacttcgt ccgctggctc tatcagttcg ccctctgccg
     6001 cgagccggca gctgaagagc ttgcgattct gaaggagtca ctgggcgaga aactgaccgc
     6061 ccagggcatc gaagacgcgc tgtggacggt gatcatgttg cccgaatttc aactggtgcg
     6121 ttagtcatgc cgaaaccatc aaagccgaaa gacatcgatg cctatttcgc acagtttccc
     6181 gccggcgtgc agcgcatcct gaagaaagtg cgccagacga ttcgccgcgc agcgccggac
     6241 gccgaggaag tcatcagcta ccagatgccc gccttcaggc agcacgggat tctcgtctat
     6301 ttcgccgcct ggaaggatca catcgggctg tatccgccga tttccggtga cgcggccctc
     6361 gagcgagcgg tggcccgcta cgctgggccc aaaggcaact tgcagtttcc tttggacgaa
     6421 ccgatccctt accacttgat cgcccggctc gtaaagctcc gagtcaaaca ggacgcggcc
     6481 aaagcgaatg ccaaacgcaa ggggcgaaag tcaggttagt tacgacaacg gagtgggcct
     6541 tacgaaggtc agcccgttag tccagcggat gctgaactgc taatatgaat tgcacgggag
     6601 tccgtccatg atccacgaca acccgaaccc tcaagcccgc cgccgcttcc ttcagcaagt
     6661 gtccgccgcc ggcgcggcgg cactcttggc cggcgagccg cggctgcttc tggctgcgcc
     6721 cggcggcgag aagctcgtgc atccggcggc gaaggccgat gcggtgatcg tgctgtggat
     6781 gggcggcggg atggctgctc cggatacgtt tgacccgaag aaatatgtgc cgttccaggt
     6841 tggcctgccg gtgggcgatg tcgagagctg ctttccggcg atcgatacgg tcgttgacca
     6901 catcaaaatc acgcagggcc tggagcagat cgcccaggtg atggatcggg ctacgctgat
     6961 ccgctcgcat gtgctgcccg acctggggag catcctgcac tcgcggcatc agtatcactg
     7021 gcacaccggc tacgtcccgc cgcaaaccgt cgcctgcccg catattggcg cgtggctggc
     7081 ccgcgtcaag ggcccacaga accccgtcat cccgccgttc atcaacattg gccagcgact
     7141 agaaggcgtc ggagagcagg aagagctgaa ggcgtttacg actgccggct tcttcggcag
     7201 cgagttcggc ccgatgaacc tgccttaccc ggaagaggcg gcccagtcgg tccgcccgcc
     7261 caagggtatg gagggggacc ggttcagcag ccgctaccgg ctctatcaga agctgctcag
     7321 agaatcgccc gaccgcgacg cgcggagcga ctatcagcgc gagtcgatgc tgcgggcgct
     7381 cgacaacgcg caccgcctgc tcagcgcgcc cgagcggacc gcgttcgaca tttcgctgga
     7441 gcccaaggag agctacgaga aatacgacac cggccgtttt ggccgcggct gcctgctggc
     7501 ccgccgcctg atcgaagccg gcgcccgcta catcgaagtc accacggagt acgtgccgtt
     7561 cctcaactgg gacacgcacg ccaacggcta cacgacgctg gtcgagatga agaagcagat
     7621 cgatcggccg atcgcccagc tcattcgcga cctggaggca cgccagctgc tcgaacgcac
     7681 gctggtcatc ctggccagcg aattcagccg cgacatgctg atcgagggcc ggccgggcag
     7741 cacggcgaac gaccaggcga cggagaaagt tgatcgcttc caggcgctca agcactacgg
     7801 gcagcaccgg cacttcacgg gcggctcgtg cgtggtgatg tggggcggcg gcgtcaagcg
     7861 gggcttcctc tatggagaga cggcgaagga gcggccgttt ttggccatca agaatccact
     7921 tacggtcacc gacctgcacg ccacgatctt cacggccctc ggcgtcagcc ccaagactgc
     7981 cttcgacgtt gagaagcgcc cgttctacgc caccgaagac ggtaagggcc agccggccct
     8041 cgagctgttc ggcgcttgaa aatcaggcga caaacgccag ccagtagaag aaccatcccg
     8101 tcacggcggt cagcgtcatt cccgccgccg ccagccagcc ggctcgggcg tgccagcggc
     8161 tgtgcgggcc cggctgcggt gggcggctga acttccgcag cgcctgaacg acgacgagaa
     8221 tccacaggca gaaagtcggc accgcgaagc agaggtgcac gatcaggctg attccgaccg
     8281 ggcaactcca tttgtgcgcc gcgtcgaaat agggcgaggc cgccgcgcga tcctcccagt
     8341 tcgtgaagag ctgcaagtcg atttcgaaca gcccgaccgc cgtcagcagc acggccgcca
     8401 tcgccagctg cagccgcttg tgcagcaggt attgccggcg ctgtttcacg agccagatgc
     8461 tggccgccag gcaggggacg atgacgaaca tcgccaggaa aacgacatcc agcatcagcg
     8521 agccgcgggt gcccaaaaac ccgcgggagc cggaagattc ttgcgccagc aggatcacct
     8581 gcggcaggcg gaagatgtcc atgcaatcgg cgaaaggaga acgtcgctca agcagcgggg
     8641 gcggaccttg gcgagccccg cggcccgcgt tagtctgacc gcgagattgt actccatcgg
     8701 ccggatggcg gaattggcag acgcacagga cttaaaatcc tgtgtcccgt taagggacgt
     8761 gcgggttcga gccccgctcc ggctattgaa taattattta ataactcgca acgagtcgaa
     8821 ttggttcgcg aagtgtcgag tggtttcgag tcagtgcacc tgacctgctt cccatgccgc
     8881 aagtgctctc cttcctcccg gaaggccggc tggcctcctg gggcggcgcg ggcggctcgc
     8941 tggtcatcgc cgacgtggat cgccgcctga ccttcgccta cgtgatgaac ttgatgaggc
     9001 ccgccctggt cgggcccgtc gcggaggcgc tggtcaagcg tctgtacgac atcgtgagcc
     9061 accctgctgg cagctagttc gggcttaccg ctgggcctga gattgggccg tgcgggtggc
     9121 cggtgcggtc acgcagccga gcgggcggca ggcgtgctgc tcgctccagc gaatgccctt
     9181 caggctggcg ccatgcttcg agacgacgtc gcggatttgg ccatagctcg gcagctgctt
     9241 ggcgctggcc ggcgactcgg acttggtggc ctggtcagag gacaggtcgt tgcggtgaaa
     9301 cttgtcccag cccaagccca cggattcgag cttcaggctg ccgagctcgg cgggatacgg
     9361 catgtccacc gtgaccagga ggaggtcctt gtcgtctttc gactcagcga acgagacgta
     9421 gttcatcgcc tccaggtccg cgaacagcgc ctgccgcgat gccttgcggg gatc
//
LOCUS       DASRJB010000437        16827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3222481, whole genome shotgun sequence.
ACCESSION   DASRJB010000437 DASRJB010000000
VERSION     DASRJB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16827
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222481"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFB80_11450"
     CDS             <1..495
                     /locus_tag="VFB80_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34430.1"
                     /translation="RFASTILPPGQPDPANPNVNGPWEDFQTSEALYYNQMFGGQLGA
                     RLCNQFGHWLLSAEVRMFGLVNFQELENIERLNRVRYTALAGDVDLQLNTETHTFANN
                     TQFVWGGEVRAEAAYEVTRDVSFRFGLLFIDLGQGIGRGNTLRFDNQDVVMTGLTAGL
                     TINR"
     gene            complement(511..693)
                     /locus_tag="VFB80_11455"
     CDS             complement(511..693)
                     /locus_tag="VFB80_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34431.1"
                     /translation="MLTESEVQRSFRNLFKSKDVASDVFERAEALLDELRAESPLRHR
                     LSLELEEIRKLHTAKV"
     gene            853..2793
                     /gene="selB"
                     /locus_tag="VFB80_11460"
     CDS             853..2793
                     /gene="selB"
                     /locus_tag="VFB80_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZN34432.1"
                     /translation="MSVDLILGTAGHIDHGKTSLIRALTGVDTDRLPEEKRRGITIDL
                     GFASLDLPPYRLGIVDVPGHERFVRNMLAGATGMDLAMLVVAADDSVNQQTREHLEIL
                     RLLDLKGGVIVVTKCDLAEENWLALVEDEIRTLAADTPLADAPLVRTSIVTGAGLAEL
                     KAALGKAAEAAAPRIAPVGRGPFRLAIDRTFAVEGHGTVVTGSVTSGRVQVGDELRIE
                     PAGLTARVRGLHNHDQPATELHRGQRGAINLGGVHHSQIERGQELCTPGALVPAWRLT
                     ARLRLLPDAPPLRDRARVRLHLGTAEILATVRLLDRSRLAPGESAFVQLHLATPAVAT
                     WNQPLVVRRESPVETIGGGRVLEPAAHAIGKLDEETATRLARLESADAIERAAATLYL
                     AGLHGWQPEALPRLAGIDDAAAACELLRSRGEMVEIALSPTNTRRLHRTILAALGERI
                     AAVLESIHRREPLRLAVPRPELSSRLSAYDDDVLAAAIDFLVKQKRVEITRLGLVLAG
                     HGPQLSRGQQQLLEQMLEWFREAGLASPGLEECTRRALKNKDSVPQLLQLAAASGQLV
                     AVGPDYWLHPAVEAQARGQVQSALAGGKSATLSEIREWLGTTRKYAVPLCEFWDQAGF
                     TVRRGDARVLGSPPIVAASSGQ"
     gene            2821..4245
                     /gene="selA"
                     /locus_tag="VFB80_11465"
     CDS             2821..4245
                     /gene="selA"
                     /locus_tag="VFB80_11465"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZN34433.1"
                     /translation="MSSVLRSIPSVTELLDSPPLKSLVNRVSRNVVVSGVRQFLEDMR
                     SQVQTAAAGVHVPTASELAQRIADWIATEELPSLRPVINATGILLHTGLGRAPLAEEA
                     IAAIGEVSRGYASVELDLASGERSQRAVAVERQLTRLTGAEAAAVVNNNAGATLLTLA
                     ALATGREVIVSRGQLIEIGGSYRLPEVMTASGTVLREVGTTNKTRIGDYAAAIGEKTA
                     ALLRVHTSNYAVVGFTEETPLAELVALGRKHDLPVIDDIGSGALVDLSPYGVKGEPIA
                     GESIRAGADLVLFSGDKLLGGPQCGIVAGRRNLVQQILKHPLTRALRVDKLTLAALAA
                     TLRLYEDPAHAERSVPLLTLLATPLENLRHRAERLAPQIQATGVAAVEVVTDRAYVGG
                     GSLPNQSLATICLALTPATGTVDALAAALRRGTPAVVGRIQEGRLLLDLRSVQPRDDT
                     HLVAAVEALRKVKNELPAELTPVG"
     gene            4379..5224
                     /locus_tag="VFB80_11470"
     CDS             4379..5224
                     /locus_tag="VFB80_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34434.1"
                     /translation="MRRSTFGPAVLALLASCALASGQEAGYPRDHHPWGRFPVGSWKF
                     VRTSSESIDEKGQITNLSSTDTRATLLSADDAAYTLRSEITVNIAGRRIPMTPQTVKH
                     GYYGEPAGRPLAIKQLGEAPVTIDGKTIPCEVRQVAVDVEGGKLTSTLHYTSLVAPFV
                     LRRESSLEGVPEEKRNATLVEVIALDLPQKIKGDIKPAMYVKTTQTLPHGKKITLEVH
                     CDDVPGGVVAHWASETDSTGRVVRRSTLELVDYGLPVVMGEPVQLTPRRAHRAAKAAR
                     RMDGR"
     gene            5285..5515
                     /locus_tag="VFB80_11475"
     CDS             5285..5515
                     /locus_tag="VFB80_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34435.1"
                     /translation="MKFIELTGKALKRLIETDELHDGDWASSGIDDDTIVRINRQGDI
                     EVRRTDRWDVVGGLLGNYEQRIKSETGLDWAE"
     gene            complement(5516..6631)
                     /locus_tag="VFB80_11480"
     CDS             complement(5516..6631)
                     /locus_tag="VFB80_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34436.1"
                     /translation="MRMFLASLIAVALMGRGPLAAQDADLFNKLDANKDGAISLDEVP
                     AEHAAKFAQLLKIAGKETEKKISLPLFQAALKQVQAAERAASQPAAAAPGPAVDADAL
                     FAKLDANKDGFVTNEEVEGGQKSLFDRLVRNADRNSDKQLSLAEFKAGLAPDDSPRQP
                     LAGGGFPGRPGGGAPQANPRQFFDRLDANKDGKLSKEELPERMREGLARLDADGDGSI
                     SPDELARSVGRAGAPGTPAPPQLAEQFDRLDANKDGKLTKDEVPEDRRLMRSIFDRAD
                     AAGVTKEQFLRAMAAVAPPGPPQRRPDGSPMPPIDALRPALAAIDANRDGELSKEEIE
                     GAAKALLALDKNGDGKLTRDELAPAPGPGRDRRPENR"
     gene            7376..7705
                     /locus_tag="VFB80_11485"
     CDS             7376..7705
                     /locus_tag="VFB80_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZN34437.1"
                     /translation="MPRRDSRTGNWQRETTAMYIYGTSQVHAAQPLNAPHRLTPAAPP
                     SHSYSTSGVDELDISPEADFVAQARELPEIREDRVAEIRAQIEAGTYETAGKLDAALS
                     RLLDEIA"
     gene            complement(7706..8044)
                     /locus_tag="VFB80_11490"
     CDS             complement(7706..8044)
                     /locus_tag="VFB80_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZN34438.1"
                     /translation="MTANEFRRLALSLPEAAEAAHMDHPDFRVGGKIFATLGFPGEAW
                     GMVKLPPGEQATFLAAQPEVFRPANGAWGRRGCTIVQLKKAKKSLVKEALAIAWRSTA
                     PPKLLKEHDP"
     gene            complement(8047..8793)
                     /locus_tag="VFB80_11495"
     CDS             complement(8047..8793)
                     /locus_tag="VFB80_11495"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HZN34439.1"
                     /translation="MLRLNVFVLLTAALVLTVRGDEPKPQPPTGIPSEAANKDLVRLS
                     EKGEIWLDLKRKAVIADGQVCLREGTLEMFACPKGTKEHESVVSLNCRAQDVHAGLLR
                     VGAISGTPVKFDPEYKPATGTIVDIYVLWKDPDGKARQVRAQEWVKNTKTGKPLEYDW
                     VFAGSGFWKDEETGQQRYQAEAGDLICVSNFPSATLDLPIESSQANDSLLYTVFTANV
                     PAKGTKVRVVMIPRPKADEKKPAADNAPPK"
     gene            9562..10074
                     /locus_tag="VFB80_11500"
     CDS             9562..10074
                     /locus_tag="VFB80_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34440.1"
                     /translation="MSRPSSGGFVLGFAAEIAIVVAVVSLLPKLDLSGGRAVPAADLP
                     PSLVSQPQETSYYERRTENAPPLREPAVARRDLPVLREPPPLIAADPSRPAFVERRLD
                     QASQKLVNGLGSYVARSADNLNLPPPPNYSPLPPAAAPNQPTTAPDFATASFPTQPVP
                     KSPPRPWMKY"
     gene            10128..10964
                     /locus_tag="VFB80_11505"
     CDS             10128..10964
                     /locus_tag="VFB80_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN34441.1"
                     /translation="MTDPAGPSWLEIVNPSHPRGPFRCIVFDFDGTLSLLRGNWQGLM
                     VPMMVETLRATGTGESAAELTALVEEFVTRLTGQPTMQQMEALASEVAQRGRPRPDPA
                     VYLARYLDQLLSRTAARIAAVRSCRSKPEEMLIPGTLPLVEHLHGRGVILVIASGTDW
                     KHLVSETAVLELERYFGDRIFGPLDNDPRFSKEAVLRQLMARYDLRTGEIAAIGDGPA
                     EMLAAKAVGGLALGVASDEVHQSGRINPLKRDHLLRAGADLIIPDYRELPTLLRLLTP
                     DS"
     gene            10973..11944
                     /locus_tag="VFB80_11510"
     CDS             10973..11944
                     /locus_tag="VFB80_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34442.1"
                     /translation="MLPTFDRSRLIVKPLAERKHDISIAQQLPLEELPPPLDERATAD
                     ITELGRRMAAARQRKAAILLLMGGHVIRAQVARQLIDLMERGLITHIGMNGAGPIHDY
                     ELARVGATCESVARYIRSGEFGLWRETGEMNDIVRDGASRGYGLAESIGRHILASDYP
                     HKDFSLLAAGVRLNVPVTCHIGIGYDILHEHPNFDPAAFGIASYRDFLAVCHTVDRLE
                     GGVFLCMGSAVMGPEVYLKALSMARNVAHQEGRRIANFTTAAFDLIPIGGDWQAEAPK
                     TNPQYYYRPWKTILVRTVADGGHSFYICGDHRQTVPYLRRSAIGASP"
     gene            12240..13313
                     /locus_tag="VFB80_11515"
     CDS             12240..13313
                     /locus_tag="VFB80_11515"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZN34443.1"
                     /translation="MRSRFVMTTVVAVALACLAAPLLAQQPGGGQGGQGGQRGGRGGG
                     GFGGFGGGGGGFGFGGGGGTSRLTLLNVEAVQKELELVDEQLTAINKVREELRPMRGR
                     GGDGQPGGRGRGGDGQPGGRGRRGGGDNPPTNPPRNPPGASLSSPAEWYFVQQEAQPP
                     QRRGGGGAGGAGGGGFGGFQLTEEQRAEFEKQRLERLKQEKDKLAEILLPHQMKRLNE
                     IYYQQLGASAVDDPDVVAELKITDDQKKKFATVRTENQEAMRTAMQEIFQGGNRGAGG
                     GGFQMTDEQRAKMTEARKKNDDKIFAVLTEEQRKKLDELKGKAFAMPEGALRGRGGFG
                     GGGAPGGAPGGRGRGGNNPNPNP"
     gene            13444..13962
                     /locus_tag="VFB80_11520"
     CDS             13444..13962
                     /locus_tag="VFB80_11520"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN34444.1"
                     /translation="MAELQVHQSALLTDLALCVNRYRLFSPGWYVCVLAKVQLTPDEW
                     EVPGLVAMVNYGRQKQCAVEGEVFHGPPNFVLDVFDSGTDEDFLRRRERFERHGVQEY
                     VVALNREIVQFLWHQLEGGSYRLLQPDEDGIIRSRALPNFWVPLAALQDRDWWAALGC
                     IERGVSRRADFS"
     gene            13984..14871
                     /locus_tag="VFB80_11525"
     CDS             13984..14871
                     /locus_tag="VFB80_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HZN34445.1"
                     /translation="MVNPGDVFAVQLPDGRFGAVRVLQTIGRSSLVCTSPYLGRERPD
                     LSEPQLRKTVVKTRFFFKSEPARRWLDGQPPPNFEFLGNLPPAKAETKLECNVYGGKW
                     GESEGQDAFLEWRWLHDRAAFEAEVRQQEAAFERRRQQPQKPKKMMSEEDFWSIIALL
                     DWKQQGNDKKVLAPAVKALATKSKVAICQFEERLAYLLYQLDTKAHARHSTASGDLDS
                     NYLSADGFLYARCVVVVNGKKFYESVLKNPRKMPKEMEFESLLSLARSAYEKKTGEEL
                     DYSTGCSYESFSNVAGWGE"
     gene            complement(15145..15417)
                     /locus_tag="VFB80_11530"
     CDS             complement(15145..15417)
                     /locus_tag="VFB80_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34446.1"
                     /translation="MIAESNAATEVDILAQVVSPDDPTFQHEFARALLELKFKDSAQA
                     RIRELLAKNNQGTLSEAERSLLEKYLRVGQFLDLLQAKARVTLSQS"
     gene            15514..16098
                     /locus_tag="VFB80_11535"
     CDS             15514..16098
                     /locus_tag="VFB80_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZN34447.1"
                     /translation="MAATSLVRSPELMNRDDAALLVVDMQAKLLPLIAGHQRVIWNCR
                     RLIDGAKVLGVSLAATEQYPQGLGPTAAELAERLGTIPAKLAFSCGECGEIFSAWREK
                     GIWKILVCGIETHVCVGQTVHDLLAEGFRVYVAADAVGARGAADHEIALRRMDSSGAT
                     LTTTEAALFEWCGRAGSPEFKQISQLVRETPPNQ"
     gene            16112..16528
                     /locus_tag="VFB80_11540"
     CDS             16112..16528
                     /locus_tag="VFB80_11540"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34448.1"
                     /translation="MSTLAAVPLQLAPGGALREPALLETSLDLLCEYALTAPQWRIDR
                     WSFVAQASRRVLVRGSPLPPLPGTHWVETEGICVPAGRSWSPAVEPAVVRQLLGLEPG
                     VLALLREDATWDRINPDDWVRASRSAFRRTLEALPR"
     gene            16525..>16827
                     /locus_tag="VFB80_11545"
     CDS             16525..>16827
                     /locus_tag="VFB80_11545"
                     /inference="COORDINATES: protein motif:HMM:NF039748.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34449.1"
                     /translation="MTLDHVQLRSYFAPPAKSFWRWQDDGFVATWKDELTIAFREELL
                     GVLDRLAAPGMPPLASVLLLLAACRDNWEEPPDRRTLLHTHLGLLYGGGYADLLAEV"
BASE COUNT         2907 a         5474 c         5513 g         2933 t
ORIGIN      
        1 cggtttgcgt ctacaatcct tcctcccggc caacccgatc ctgccaaccc caacgtgaat
       61 ggcccgtggg aagactttca gacgtcggag gcgctgtact acaatcagat gttcggcggc
      121 caactcgggg cgcggctctg caatcaattc ggccactggc tgctgtcggc ggaagtgcgg
      181 atgttcggcc tggtaaattt ccaagagctc gagaacatcg aacggctcaa tcgcgtccgc
      241 tataccgcgc tggccggcga cgtcgacctg caactgaaca cagaaacgca cacctttgcg
      301 aacaacacac agttcgtgtg gggcggcgaa gtgcgggcgg aagcggccta cgaagtgacg
      361 cgcgacgtct cgttccgttt tggcttgctg tttatcgatc tgggccaagg catcggccgc
      421 ggcaacacgc tcaggttcga caatcaggac gtcgtcatga cgggcttgac ggccggtctg
      481 acgatcaacc gctgaattag tggcggaggc tcagaccttc gccgtgtgca gcttgcggat
      541 ttcttcgagc tccaggctca agcgatgacg cagcgggctc tcggcccgca attcgtccag
      601 cagtgcctcg gccctttcaa agacgtcgga cgctacgtcc ttgctcttaa acagattacg
      661 aaagctacgc tgcacctcgg attcagttag caagggatac tcctcacacc ggcccagtag
      721 agatggcaga cgaacctgcc cacggtcacc ataccttaat tctattggac gcgggcggtg
      781 acgcaagtgc gactgagcgg ctgaagcccc gcgcgagtgc agcggcgtgc cagcagccct
      841 tatactcgcc gtatgtccgt cgatctgatc ttgggaaccg cggggcacat cgaccacggc
      901 aaaacctcgc tgattcgggc cctcaccggc gtggataccg accgcctgcc cgaagaaaaa
      961 cggcggggca tcacgattga cctgggattc gcttcgttgg atttgccccc ataccggctg
     1021 ggcatcgtgg atgtgccggg ccacgagcgt tttgtccgca acatgctggc cggtgctacg
     1081 ggcatggacc tggcgatgct cgttgtcgcc gccgacgact cggtgaatca gcagacccgt
     1141 gagcatctgg aaatcctgcg gctcttggat ctcaagggcg gcgtgatcgt tgtgaccaag
     1201 tgcgacctgg cggaggaaaa ttggttggcg ctggtggagg acgagattcg cacgctggcc
     1261 gccgacactc cgctggccga cgcgccgctg gtacgaacga gcattgtcac cggggcgggc
     1321 ctcgctgaat tgaaagccgc tctgggcaaa gctgccgaag ccgccgcgcc gcgcatcgcc
     1381 cccgtgggac gcgggccctt tcgtctggcc atcgaccgca cgtttgccgt cgaaggtcac
     1441 ggcacagtgg tcaccggcag cgtcaccagc ggccgcgtgc aggtcggtga tgagctgcgg
     1501 attgagccgg ctggcttgac cgcgcgcgtc cgcgggctgc acaaccacga tcaacctgcg
     1561 acggaactcc atcgcggtca acgcggcgcg atcaacctgg gcggcgtcca tcattcgcag
     1621 atcgagcgcg gccaggagct ttgcacaccc ggcgcgctcg tgccggcgtg gcgtctgacg
     1681 gcccggttgc gcttgctgcc cgatgctccg ccgctgcgcg accgggcccg agtccgcctg
     1741 cacctgggca cggcggagat tctggcaacg gtgcgcttgc tcgaccgcag ccggctagcg
     1801 cccggagaat ccgccttcgt gcagttgcac ctggcgacgc ccgccgtggc gacctggaat
     1861 cagccgctgg tcgtgcgccg cgaatcgccg gtcgaaacga tcggcggcgg gagagtgctc
     1921 gagccggcgg cgcacgccat cggcaaactg gacgaagaaa cggcgacgcg gctcgcgcgg
     1981 ctggaatcgg ccgatgccat cgagcgcgcc gcggcgacgc tctatctggc cgggctgcac
     2041 ggctggcagc cggaagcctt gccgcgcctg gcgggcatcg acgacgcggc ggccgcttgc
     2101 gagctgctcc ggtcgcgcgg cgagatggtc gagatcgccc tcagcccaac gaacacgcgc
     2161 cgcttgcacc ggacgattct ggcggcgctt ggcgaacgca ttgctgccgt gctggagtcg
     2221 atccaccgcc gcgagccgct gcggctggcc gtgccgcggc cggaattgtc gtcgcggctg
     2281 tccgcctacg acgacgatgt gctggccgcg gcgatcgact tcctcgtgaa gcaaaagcgc
     2341 gtcgagatca cgcgtctggg actggtgctc gcgggccacg ggccgcagct ctcgcgcggg
     2401 cagcagcaat tgctcgagca gatgctggag tggtttcgcg aggccggcct ggcttcgccc
     2461 ggcctggagg aatgcacgcg gcgggctctg aagaacaaag actccgtccc gcagctcctg
     2521 cagctggccg cggccagcgg tcagctcgtc gccgtcggtc cggactactg gctgcatccc
     2581 gccgtcgaag cgcaggcccg cgggcaagtg cagtcggcgc tggccggcgg caagagcgcg
     2641 acgctgagcg agattcgcga atggcttggc acgacgcgca agtacgccgt accgctctgc
     2701 gagttttggg accaagcggg gtttaccgtc cgccgcggcg atgcccgcgt cctgggttcg
     2761 ccgcccattg tcgccgcgag cagcggccag tagtttgaac tttcaatcgc gggagtcccc
     2821 atgtccagcg tccttcgcag tattccgtcg gtcaccgagc tgcttgacag cccgccgctg
     2881 aaatcgctcg tcaaccgcgt cagccggaac gtggtcgtgt cgggcgtgcg gcagttcctg
     2941 gaagacatgc ggtcgcaggt gcagacggcc gcggcagggg tccacgttcc aaccgcgtcg
     3001 gagttggccc agcggatcgc cgactggatc gccaccgagg agctgccgtc gctgcggccg
     3061 gtcatcaatg ccacgggcat tctgctgcac accggtctcg ggagagcgcc cctggcggaa
     3121 gaggcgattg cggcgatcgg cgaagtctcc cgcgggtacg ccagcgtgga gctcgacctg
     3181 gccagcggcg agcgctcgca gcgggccgtc gccgtcgagc ggcagctgac gcggctcacc
     3241 ggggctgagg cggccgccgt cgtgaacaac aacgccggcg cgacgctgct cacgctggcc
     3301 gccctggcga ccggccgcga agttatcgtc tcccgtggcc agttgatcga gatcggcggc
     3361 agctaccgcc tgcccgaagt gatgaccgcc agcggcacgg tgctccgcga agtcggcacc
     3421 accaacaaga cgcggatcgg cgactatgcc gcggcgatcg gcgagaaaac ggccgctctg
     3481 ctgcgcgtgc atacgagcaa ctacgcggtc gttggattta ccgaagagac gccgctggcg
     3541 gaattggtcg cgctgggccg caagcacgac ctgcccgtga tcgacgacat cggcagcgga
     3601 gccctcgtgg atctttcgcc ctacggcgtc aagggagagc cgatcgccgg tgagagcatt
     3661 cgcgccggcg ctgacctggt gctgttcagc ggcgacaaac tgctgggcgg tccgcaatgc
     3721 ggcatcgtcg ccggccgacg caatctcgtg cagcaaatcc tcaagcaccc gctcactcgt
     3781 gccttgcgcg tcgataagct cacgctggcc gcactggcgg cgacgctgcg gctgtatgaa
     3841 gatccggcac acgccgaacg ctccgtgccc ttgctcacgc tgctggctac accgctcgaa
     3901 aacctccgtc accgcgccga gcgactcgcg ccgcaaatcc aggccacggg tgtagcggcg
     3961 gtcgaggttg tcaccgaccg cgcctatgtc ggaggcggtt cgctgccgaa tcaatcgctg
     4021 gcgacgatct gcctggcact cacgccggcg accggcacgg tcgatgcgct ggccgcagcg
     4081 ctccgccgcg gcacgcccgc cgtcgttggt cgaattcagg aggggcgctt gttgctcgac
     4141 ctgcgcagcg tgcagccgcg agacgacacc cacctcgtcg cggctgtcga agcactccgc
     4201 aaggtaaaga acgaattgcc agccgaactg acgcccgtcg gttgatttgc acgatccgca
     4261 ggaccagccg cgttcaagca gaatttcgca ggaaaaaagt gcggcaaagg gtcgaatcgt
     4321 ctcgtgccga gggttaaact ttcggaatcg aaaaaccaag gcggacctgc ttcactctat
     4381 gaggcgttca acattcggcc cggcggtgct ggctctgcta gcgtcgtgcg ccctggccag
     4441 cggccaggaa gccggctatc cacgcgacca ccatccctgg ggccgcttcc ccgtcggctc
     4501 gtggaaattc gtgcggacgt cctcggagtc gatcgacgag aagggccaga tcaccaacct
     4561 ctccagcacc gacacgcgcg cgaccctgct gagcgccgac gacgccgctt acaccttgcg
     4621 gtcggagatt acggtgaaca tcgccgggcg gcggattccc atgacgccgc agaccgtcaa
     4681 gcacggctat tacggtgagc cggccggccg gccgctggcg atcaagcaac tgggcgaagc
     4741 gccggtgacg atcgacggca agacgatccc ctgcgaggtc cgccaggtgg ccgtggacgt
     4801 cgaaggaggc aagctgacca gcacgctcca ctacaccagc ctggtggccc cgttcgtgct
     4861 gcgccgcgaa tcgagcctgg agggcgtgcc ggaggaaaag cgcaatgcga ccttggtgga
     4921 agtgatcgcc ctcgatctgc ctcagaagat caagggggat atcaagcccg cgatgtatgt
     4981 gaagacgacg cagacgctgc ctcacggaaa aaagatcacc ttggaagttc actgcgacga
     5041 cgttcccggc ggcgtggtcg cccactgggc cagcgagacc gactctaccg gccgcgtggt
     5101 ccgccgcagc actctggagc tcgtggacta cggcctgccg gtcgtcatgg gcgagcccgt
     5161 gcagctcacg ccgcgccgcg cgcatcgggc cgcgaaggcc gcccgccgga tggacgggcg
     5221 atagcggctc gccctcgctc ccatcgcgag ttaggatgac cgagtccacg acgcgaggag
     5281 cggcatgaaa ttcatcgagc tgaccggcaa ggcgctcaaa cgccttatcg agacggacga
     5341 gctgcacgac ggcgattggg cttccagcgg catcgacgac gacacgatcg tgcggatcaa
     5401 ccgccagggc gatatcgaag tccgccgcac cgaccggtgg gacgtggtcg gcgggctgct
     5461 cggcaattac gagcagcgga tcaagtcgga aacgggactg gattgggccg agtaatcagc
     5521 ggttttccgg ccggcggtcg cgtcccggcc ctggggcagg cgcgagttca tcgcgggtca
     5581 gtttgccgtc gccgttcttg tccagcgcga gcagggcctt cgccgcgcct tcgatttcct
     5641 ccttggatag ttctccgtcc cgattggcat cgatcgcggc cagtgccggc cgcagtgcgt
     5701 caataggcgg cattggcgaa ccgtccgggc ggcgctgcgg cgggcccggc ggggcaaccg
     5761 ccgccatcgc ccgcagaaac tgctccttgg ttactcccgc ggcatcagcg cggtcgaaga
     5821 tgctccgcat caggcgccgg tcttcgggca cttcgtcttt ggtgagcttg ccgtccttat
     5881 tggcgtcgag tcggtcgaat tgttcggcca gttgcggcgg cgcgggagta cccggtgcgc
     5941 cggcccgccc cacggaccgg gcaagctcgt cgggactgat cgatccatcg ccgtcggcgt
     6001 cgagccgggc gagtccttcg cgcatccgtt ccggcagctc ctccttgctc agcttgccgt
     6061 ccttgttcgc gtcgagccgg tcgaagaact gccgcggatt ggcctgcgga gcgccgccgc
     6121 cgggccggcc gggaaagccg ccgcccgcca gcggctgccg agggctgtcg tccggcgcga
     6181 ggcccgcctt gaattccgcc aggctcagct gcttgtcgct attgcggtcg gcgttgcgga
     6241 ccaggcgatc gaagagcgac ttctgcccgc cctccacttc ctcgttcgtc acgaagccgt
     6301 ccttgttcgc gtccagtttg gcaaacagtg cgtccgcatc gacggcgggc ccgggggcag
     6361 ctgccgcagg ttgcgaggcc gccctttccg ctgcctgaac ttgcttgagc gccgcctgaa
     6421 acagcggcag gctgattttc ttttccgtct ccttgccggc gatcttcagc agctgcgcga
     6481 acttcgccgc gtgctcggcc gggacttcat caagcgagat agcgccgtct ttgttcgcat
     6541 cgagcttgtt gaacagatcc gcatcctgag ccgccagcgg cccgcggccc atcagggcga
     6601 ctgctatcag actcgcgagg aacatccgca tgatcgtgct ccgatctcga tgacgtaaag
     6661 cctggtgaac gaaagttaaa ccgcagccgc tgcgcaaggt ttcgcggctg cccaagttat
     6721 gccgtcgatt gtctccgcct aagctgctgc ggtcaaacgc cttagggcga gcttgccgct
     6781 ccaccgccgt tttcctatac tccgccacgg aatttcgcgc cggtggaaaa ccaaccgcct
     6841 ggcgcggacg tatgggttgg catggtgctg cggcgctcgc cgcagtgcag cttcccgctt
     6901 tcacaaccac tgggaaagga gtcccgcttc tgagccggtt gccgatcggc aacacgcctg
     6961 catcgcgaaa gccacatcgc gttcatcgcc gcaacaaggt tgccgccagt caacgcgaat
     7021 gtgcccgcac aaaagccttt cgccgcagtc gctgttccgt tcgccacgcc cggcacattc
     7081 cgcggagacc aagggaaccg gtacaggagg ttaccgaaca acttgggtgg atgggccgcc
     7141 cgactccggc cgatgggagt gctatcacgc tgacgctggc aagtctcgct tgcttgtcac
     7201 ggacgctccc caaggcacgg atcacggcgc tcagcaccac ctgcaacgta cacttggggg
     7261 acgcagtcgt ccgtgaagat tggcccgcgc gaagaaccgg tggcccgcca ccggtcgatc
     7321 gcccaccgct tctcagcgac ccctcgtccc gccgtttcga tccaagggat tcgccatgcc
     7381 gcgccgcgac tcgcgcaccg gtaactggca gagggagacg accgccatgt atatttatgg
     7441 aaccagccag gttcacgccg cccagccgct caatgctccg caccgcctga cgccggctgc
     7501 gccgccgagc cattcgtact ccaccagcgg agtcgatgaa ttggacattt cgcccgaagc
     7561 cgacttcgtc gcccaggccc gcgagctgcc cgagatccgc gaggatcgcg tggccgagat
     7621 ccgcgcccag atcgaagccg gcacctacga aaccgcgggc aagctcgacg cggccctcag
     7681 ccggctgctc gacgagattg cgtagctacg ggtcgtgctc tttcagcagc ttcggcgggg
     7741 ccgtgctccg ccaggcaatc gccagcgctt ccttcaccag cgatttcttg gccttcttga
     7801 gttgcacgat cgtgcagccg cggcgccccc atgctccgtt ggcgggccga aagacctcgg
     7861 gctgcgcggc gagaaacgtc gcctgctcgc cggggggcag cttgaccatt ccccaggctt
     7921 caccgggaaa ccctagcgtg gcaaagatct tgccgccgac gcggaagtcg ggatggtcca
     7981 tgtgggccgc ctccgcggct tcgggaaggc tcagcgccag gcggcgaaac tcgttggcgg
     8041 tcatggctac ttgggcggtg cgttgtcggc ggcaggcttc ttctcgtctg ccttcggccg
     8101 cgggatcatg acgacgcgga ccttggtgcc tttcgcggga acgttggcgg tgaaaaccgt
     8161 gtacagcagg ctgtcgttgg cctgcgagct ttcgatcggc aggtcgagcg tggccgacgg
     8221 gaagttcgag acgcaaatca ggtcgccggc ctcggcctgg taccgctgct gccccgtctc
     8281 ttcatccttc cagaagccgc tgcccgcgaa gacccaatcg tactcgagcg gcttgccggt
     8341 tttggtgttc ttcacccact cctgggcccg cacctggcgg gccttgccgt ccggatcttt
     8401 ccagagcacg taaatatcga cgatcgtgcc cgtcgccggc ttatactccg ggtcgaactt
     8461 gacgggcgtg cccgaaatcg cgccgacccg caatagaccc gcgtgaacgt cctgggcccg
     8521 gcaattcagc gagacgaccg actcgtgctc cttggtgccc ttcgggcagg cgaacatctc
     8581 cagcgtccct tcacgcaggc agacctgccc gtcggcaatc accgctttcc gcttgaggtc
     8641 cagccagatc tccccctttt ccgagagccg cacgagatct ttgttggccg cctcagaggg
     8701 aatgccggtc ggcggctgcg gcttgggttc gtcgccgcgg accgtgagca ccagagcggc
     8761 tgtcagcaac acaaagacgt tgagtcgaag catagaaagg agccttgaat tggttgaggt
     8821 cgttggccac gcgcccggtc gcggaaaatt atatcgcccc gcgccgccgg ctgagaacgc
     8881 aattcgcgtg gcggcggccg aacagtctcg tcgcgaattg agacatcccg gccgtgggcg
     8941 gatcggacaa gccgcgccca aaccattgcc ctaaaccacg gctcgggaac attaggaaac
     9001 cgtctgaggc tcgattctct tcgcgcccca ccgcgccgcg gatcgcgcca caactgcttg
     9061 ccggcagagg acttccgcga tcctgtcggc ggatttcaga cattagagtt ataagcctta
     9121 gaaggaccgg cgggagttac ctaatgctcg gccgcgcatc agcagcggca tcgattctgc
     9181 cctagtgcaa gacaacccct tgagacagag ccggggccgg cggcgaaaat ggcggatcgc
     9241 agcgccaatt ggaactatac tgcaaatagc ataccaatgt tcacattcgc ggccgttcgc
     9301 ggtcccgccg ggcctccgtg cggtcgcgcg catctgtcat cgacttgaga atcgctcgcc
     9361 aattttgcaa cgccgttgta gctttgcggc tgctggcgcg gattgctggc aggcagcgaa
     9421 tctcgcgtgt tttgctgggt ttgagcgggc ttgaggaaaa gcgcgcgcgg tttggcaccg
     9481 tcgttgcata cggagcagtc atcactacac ggatgccgcg cggcgcacgc tagtcggccg
     9541 cgcgcaatgg aaggagtcgt tatgtcgcgc cccagctctg gcggttttgt cctcgggttc
     9601 gccgccgaga tcgccatcgt cgtcgccgtc gtctcgctgc tcccgaagct cgatctaagc
     9661 ggcggccgcg ccgtcccggc cgcggacctt ccgccgtcgc tggtctcgca gccgcaagag
     9721 acgtcgtact acgagcggcg gaccgagaac gcgccgccgc tgcgcgagcc cgcggttgcg
     9781 cggcgcgacc tgccggtctt gcgggagccg ccgccgttga tcgccgccga tccttccagg
     9841 ccggcatttg tcgagcgccg cctcgaccag gccagccaga agctcgtcaa tggcctgggc
     9901 tcttacgtgg ctcgttcggc ggacaacttg aacctgccgc caccgccgaa ctacagcccg
     9961 ctgccaccgg ccgctgcgcc aaaccagcca acgacggctc ccgacttcgc gaccgcgtca
    10021 tttccaacgc agccggtgcc aaagtcgccg ccgcggccgt ggatgaagta ttagcccggc
    10081 tggttatgat gggtctggtt acttcgcagc cgacccttca ctctcccatg accgacccgg
    10141 ccgggcccag ctggctcgaa atcgtcaatc cgagtcatcc gcgcgggcca tttcgctgca
    10201 tcgtgttcga ctttgacggc acgctctcgc tgctccgcgg caactggcag gggctgatgg
    10261 tgccgatgat ggtcgagacg ctgcgggcca cgggcacagg cgaatcggcg gcggagttga
    10321 cggcgctcgt ggaggagttc gtcacccggc tgaccggcca gccgacgatg cagcagatgg
    10381 aggcgctggc cagcgaagtc gcccagcgcg gccgcccgcg gcccgatccg gcggtttacc
    10441 tcgcgcggta tttggaccaa ttgctgagcc gcacggcggc ccgcatcgca gccgtgcgct
    10501 cctgtcgatc aaagccggag gaaatgctta tccccggaac cctgccgctg gtcgagcatc
    10561 tgcacggccg cggcgtcatt ctggtgatcg catccggcac agattggaag catctggtaa
    10621 gcgagacggc agtgcttgag ctcgagcgct attttggcga ccgcatcttc gggccactcg
    10681 acaacgaccc gcgattttcc aaagaggcgg tgctgcggca gctgatggcg cgatacgatc
    10741 tgcggacggg cgagatcgcc gcgatcggcg acggccccgc cgagatgctg gcggcgaaag
    10801 ccgttggcgg actcgcgctc ggggttgcca gtgacgaggt gcaccaaagc ggccggatca
    10861 atccactgaa gcgcgaccac ctgctccgcg ccggcgccga cctgataatc cccgactatc
    10921 gcgaattgcc aaccctcctc cgactcctga ctcccgactc ctgacccctg gcatgctgcc
    10981 gaccttcgac cgcagccgac tgatcgtcaa gccgctggcg gagcggaagc acgacatttc
    11041 gatcgcgcag cagctgccgc tggaggaatt gccgccgccg ctcgacgagc gggcgacggc
    11101 ggatattact gagctcgggc ggcggatggc agcggcccgc cagcggaagg cggcaatctt
    11161 gctgctcatg ggcggccacg tgattcgcgc ccaggtggca cgccagctca tcgacctgat
    11221 ggagcgcggc ttgatcacgc acatcggcat gaatggcgcc ggcccgatcc acgactatga
    11281 gctggcccgg gtcggcgcga cgtgcgagag cgtggcccgc tatatccgct cgggcgaatt
    11341 cggcttgtgg cgcgagacgg gcgaaatgaa cgacatcgtc cgcgatggtg ccagccgcgg
    11401 ctacggtctg gccgagtcga tcggccgcca catcctggct agcgactatc cgcacaagga
    11461 tttcagcctg ctcgccgctg gcgtgcgcct gaacgtaccc gtgacttgcc acatcggcat
    11521 cggctacgac attctccacg agcacccgaa ttttgacccg gccgccttcg gcatcgccag
    11581 ctatcgcgat tttctggccg tctgccacac ggtcgatcgc ctcgaaggcg gcgtgttttt
    11641 gtgcatggga tcggccgtga tggggcccga ggtctacctg aaggccctgt cgatggcccg
    11701 caatgtcgcc catcaggaag gccggcgaat cgccaacttc acgacggctg ccttcgatct
    11761 gattcctatc ggcggcgact ggcaggccga ggccccgaaa acgaaccccc aatactacta
    11821 ccgaccttgg aagacgatcc tcgtccgcac agtagccgat gggggccaca gcttttacat
    11881 ttgcggcgac caccggcaga cggtgcccta cctgcggcgg tcggcgatcg gcgcaagtcc
    11941 atgatactga atggcttgtg gcaggattct gggagctccg acgtgacgtc aaacgttcgc
    12001 tcgtggttag tcaagaaatc tggacgggaa gccgacagcg gcgaaacctt ttggattggt
    12061 ttctgtttaa tttgccagcg ggccgcttcg tggcgctcgc tgtgtgcttt gcgtccaaac
    12121 ccaaccggcg gcccgatccc taatccagga ctggtcgagc gggcaagggc ggggtaagcg
    12181 tcttttcggg ctctcgccgg tcggcgaggc aacttttttg ctgtgccaag gagcatgcaa
    12241 tgagatctcg atttgtgatg acgacagtgg tggccgtggc actcgcgtgc ctcgcggcgc
    12301 ccctcctggc ccaacagccg ggcggcgggc agggcgggca aggcggccag cgcggcggcc
    12361 ggggcggcgg aggttttggc ggcttcggcg gcggcggggg cggttttggc ttcggcggcg
    12421 gtggcggcac cagccggctg acactgctca acgtcgaggc cgttcaaaag gagctggagc
    12481 tcgtcgatga acagctgacg gcgatcaaca aagtccgcga agagctgcgc ccgatgcgcg
    12541 gccggggcgg cgacggccag cctggcggtc gcgggcgtgg cggcgatggg cagcccggcg
    12601 gtcgtggccg gcgcggtggc ggcgacaacc cgcccactaa tcccccgcgg aatccgccag
    12661 gggcaagcct ctcctcgccc gccgaatggt actttgtgca gcaagaggcc cagccgccgc
    12721 agcgccgcgg cggtggcgga gccggtggtg ccggcggtgg cggtttcggt ggctttcaac
    12781 tgaccgagga gcagcgggcc gaattcgaaa aacagcgcct ggagcgcctg aagcaggaaa
    12841 aagacaagct ggccgaaatc ctgctgccac accagatgaa gcggctgaac gagatttact
    12901 atcagcagct cggcgcgagc gcggtcgatg atccggatgt agttgccgaa ctcaagatca
    12961 ccgacgatca gaagaagaag ttcgccacgg ttcgcacgga aaatcaggaa gccatgcgga
    13021 cggccatgca ggagatcttt cagggcggca atcgcggggc gggcggcggc ggcttccaga
    13081 tgaccgacga gcagcgtgcc aagatgaccg aggcccgcaa gaagaacgat gacaagatct
    13141 tcgccgtgct gaccgaagag cagcgcaaga agctcgatga gctgaagggc aaggccttcg
    13201 ccatgccgga aggcgctctc cgcggccgcg gtggtttcgg tggcggtggc gctcctggcg
    13261 gcgctcctgg cggtcgcggc cggggtggca acaatccgaa ccccaacccg tagttcgcag
    13321 ccagtccacg gataaacctc agcctcgctt cgctgaagcg aggcttttct tttgcgcgct
    13381 ttggcgccag gggcgttgac gcagctggtg ccatcgccaa caaaatgacc cgcggggctt
    13441 gttatggccg aacttcaagt ccaccagtcg gcgctgctca cggacctggc gctgtgcgtc
    13501 aaccgctacc ggttgttctc gccgggttgg tacgtctgcg tgctggccaa ggtgcagctg
    13561 acgccggacg aatgggaagt cccggggctc gtggccatgg tgaactatgg gcgccagaag
    13621 cagtgcgccg tcgagggcga agtctttcac gggccgccga acttcgtcct cgacgtgttc
    13681 gacagcggca cggacgagga ctttctgcgc cgccgcgagc gcttcgagcg gcatggcgtg
    13741 caggagtacg tggtggcttt gaaccgtgaa atcgtgcagt ttctgtggca tcaactggag
    13801 ggcggcagct accgcttgct tcagcccgat gaagacggca tcatccgcag ccgggccctg
    13861 cccaatttct gggttccgct ggcggccctg caggaccgcg actggtgggc cgcactgggc
    13921 tgcatcgagc ggggcgtctc gcggcgagcc gacttcagtt gaggttcgcg aaggggtgga
    13981 gccatggtga atccgggcga cgtctttgcg gtgcagctgc ccgacggacg gtttggtgcg
    14041 gtccgcgtgc tgcagacgat cggacgcagc tcgctggtct gcacttctcc gtatctcgga
    14101 cgcgagcgac ccgacctgag cgagccgcag ctccgaaaga ccgtggtgaa aacgcggttc
    14161 ttcttcaaga gcgagcccgc ccgtcgctgg ctcgacggtc agccgccgcc aaacttcgaa
    14221 ttcctgggga atttgccgcc tgccaaggcc gagacgaagc tggagtgcaa cgtctatggg
    14281 ggaaaatggg gcgaatcgga gggccaagat gcatttttgg aatggcgctg gttgcacgac
    14341 cgcgccgctt tcgaagcgga ggtgcggcag caggaagcgg ctttcgagcg ccgccggcaa
    14401 caacctcaga agccgaagaa gatgatgagc gaggaggatt tctggtcgat catcgccctg
    14461 ctcgattgga agcaacaagg caacgacaag aaagtgctcg ccccagcggt taaggcatta
    14521 gctaccaaat cgaaggtagc aatttgccag ttcgaagaac ggctggccta tctgctctac
    14581 cagctcgaca ccaaggccca cgcccgccac agcacggctt caggcgatct ggattcgaat
    14641 tacctgtccg ccgatgggtt tctctatgcc cggtgcgtcg tcgttgttaa tgggaagaag
    14701 ttctacgaat ccgtactgaa gaacccgcgc aaaatgccca aggagatgga gttcgaatcg
    14761 ctgctatccc tcgcccggag cgcatacgag aagaaaaccg gcgaggagtt ggactattcg
    14821 accggctgca gttacgaatc gttctcgaat gttgccggct ggggggaata gcaactaggt
    14881 cgcttcatca cttcgactgc gttggcggaa acacaccggc agcgatcaat agccgtcggt
    14941 gtgcgactcg gcctggctca ttgatcgcca acacgtcgat cgtgacccgg ccgatgtcgc
    15001 tcaggccttc gagcaggcta ccctgccagc ggaaatgctc gctccaaaca tcccggcggg
    15061 gatgaaacag acgaacaagc aatcccgtct tagggtcaag gcccgacaga caagttcgcg
    15121 cagcgatgcc atggcccggc ccgttcagct ttgactcaag gtgactcggg ccttggcctg
    15181 caacaaatcg agaaactgcc caacccgcag gtacttttcg agcaagctcc gctccgcttc
    15241 gctgagcgtg ccctgattat tctttgcaag cagttcacgg atgcgggctt gtgcgctgtc
    15301 cttgaatttc agctccaaaa gtgcccgcgc aaactcgtgc tgaaaagtag ggtcgtccgg
    15361 gctgaccacc tgggccagaa tgtcaacctc ggtggcggca ttactttcgg cgatcatcgc
    15421 gggattctcc cttgtcatct gtattctatc ctttaggtag ccctaccgca cgttgcattt
    15481 cacgcccggc agcagccggg tatgttgaat ttaatggccg ctacatccct cgtccgcagc
    15541 ccggagttga tgaaccgcga tgatgcggcg ctgctcgtcg tggatatgca ggcgaagctg
    15601 ctgccgctga tcgcggggca ccagcgggtg atctggaatt gccgccggct gatcgacggg
    15661 gcgaaggttc tcggcgtgag cctcgcagcg accgagcagt atccgcaagg gctggggccg
    15721 acggccgcgg aattggccga gcggctgggg acgatcccgg cgaagctagc gttcagctgc
    15781 ggagagtgcg gcgaaatctt ttcggcctgg cgcgagaagg gcatctggaa gatcctggtc
    15841 tgcggcatcg agacgcacgt ctgcgtcggg caaacagtgc acgatctctt ggcggaaggc
    15901 tttcgcgtct atgtggcggc cgacgctgtg ggggcccgcg gcgcggcgga ccacgagatc
    15961 gccctgcggc gaatggattc ctcgggggcg acgctgacca cgaccgaggc ggcactgttc
    16021 gagtggtgcg gccgcgccgg ttcgcccgag ttcaagcaaa tcagccagct cgttcgcgag
    16081 acgccgccca accagtgagc cgcctccgcc gatgagcacc ctcgccgcag tcccgctcca
    16141 actcgccccc ggcggcgccc tccgcgaacc ggcgctgctc gaaacgagtc tggacctgct
    16201 gtgcgaatac gcgctaaccg cgccgcagtg gcggatcgac cgctggagct ttgtcgccca
    16261 agctagccgc cgggtgctcg tccgaggctc gccgctgccg ccgctcccgg gaacccactg
    16321 ggtcgaaacg gaaggcatct gcgtgccagc agggcgatcg tggtcgccgg ccgtcgagcc
    16381 ggccgttgtc cgtcagctgc tcggcttgga gccaggcgtc ctggctctgc tccgcgagga
    16441 cgccacgtgg gaccgaatca accctgacga ttgggtccgc gctagccgat cagccttccg
    16501 ccgcacgctg gaggcgctgc cgcgatgacg ctcgaccacg tgcaactgcg gagctacttc
    16561 gcgccgccgg ccaagtcgtt ctggcggtgg caggacgacg gcttcgtcgc gacctggaag
    16621 gacgagctga cgatcgcctt ccgcgaagag ttgcttggcg tgctggaccg gctggccgct
    16681 cccgggatgc cgccgctggc aagcgtgctc ttgctgttgg cggcctgccg cgacaattgg
    16741 gaagagccgc ccgaccgccg cacgctcttg cacacgcact tgggattgct gtacggcggg
    16801 ggctatgccg acttgctcgc ggaagtg
//
LOCUS       DASRJB010000438         8611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2378565, whole genome shotgun sequence.
ACCESSION   DASRJB010000438 DASRJB010000000
VERSION     DASRJB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378565"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..3431)
                     /locus_tag="VFB80_11550"
     CDS             complement(180..3431)
                     /locus_tag="VFB80_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN34450.1"
                     /translation="MYWLAELCVRRPVFALMIITSLVVAGVVAFPNLGVDRYPNMDLP
                     SVYVRTVYPGAASTEVESEVSQVLEDAVATVAGIDELRSISSDGMSMVMVTFNLTRNL
                     DAAVQDVRDAVASVRNRLPPGLDPPVVQKQDTDSSPIITLAVSGPRSASELYLLADRY
                     VKNVIESAPGVGQVQIAGAADRAVQINIDARRLAAYHLSILQVREALALQNAEVPGGR
                     VDEGLRERNLRTLGRVPHAREFADLVVTTVGNTAVRISDLGEARDASKEVRTLARLNG
                     RPAVVLQVQRQSGENTVRVIEGVKQRLERSHDLLPRDVEVTVIQDQSRYILAALHEIE
                     GHLIVGSILASLTVLAFMRSWRSTFIAAVAIPTSIVATFAFMKLFGFTLNNVTMLALV
                     LMVGVVIDDAIVVLENVFHCIEEKGMDPVAASIAGTREIGPAVLATTISLVIVFLPVS
                     FLSSVTGRMLFQFGITATVAIMVSMLVSFTLTPMMCSKLLRPARTSTAGNPASRRGFY
                     HWLEVFYMWSLRLSMRFRWAVMLLCAVTVASNYWLYDLVKQDYIPTNVDESEFEVSLQ
                     APEGASLASMHEALAKVEAEIRAMPGVELILATVGTRGFGGVNRGEMYVRLTDIRGRS
                     FSLGRWWRGLLKGDPGEAWRGNFSQRDKMAEVRQLLKKTPALRGSVRNLTSLRQGAPV
                     DIDFSITGPDINTLFDFSERVRQRAAEIPGIVDVDTTLRLDKPELLVNIDRERSAAIG
                     VEVREIAETLRVAVGGDDRVSRYRDATVDDAYDVELRLIGLDRRNIESISQLYVRANP
                     RGGRASSSGPVLTRIDNVVQFDFGEAAARIDRLDRERMVAVRANVAPGYGLASRIAAL
                     NQAVDEIGKPTGFSTRVLGRGRELERTLTDFVWTFSLSFIFMYIVLAAQYEHLVHPFT
                     ILLSLPLAVPFGLISLYWGGETLNLYSALGILVLFGVVKKASILQIDHTNVLRERGLP
                     RLEAILQANRDRLRPILMTTIAFVAGLLPLLIATGPGAEERRSIAVLTVGGQTLSLLL
                     TLLAVPVIYSYLDDLGHALTWLAAPAPARQPAPQPALNPAPLSPKSPLPLGEG"
     gene            complement(3466..4818)
                     /locus_tag="VFB80_11555"
     CDS             complement(3466..4818)
                     /locus_tag="VFB80_11555"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN34451.1"
                     /translation="MRQRGSSFSALPLAGLALLGGLTLAGCGGHGAAAPPKAPELPVL
                     KAELLTVQPRAFPTIVRAQGSLIADEVTIVGAKVGGRVAEILVDLGDTVPAGTKLASL
                     EQEEFKLQVALAEAQLVQARAALGMKPTDPVEGLTPENSPPVREARAVWNEALARVER
                     LRQIRVKNAVTQDELDTAIGAEGVANARYSAALNGVGEKIALIGVRAAELSVAKQRLT
                     DTVIHAPFDGFVQERHVALGSFVQVGDPLVTLVRAGKLRFRGTMPERYAQRLALGQEV
                     TLSIESLPAPLAVSITRLSPVVDEMSRSLVFEAEVPNQGGTLRTGLFAEAEVVIDPAA
                     QSLVVPSSAVTEFAGAEKVWKVVDGTAKEQIVQTAHRGAGGIEIVGGLKPGDVILKNG
                     AQGRVAKIEPISATPEQPQTAPVSTEPPDSTGGEGGGAEAGSEPTPPLPHTVRRPVER
                     "
     gene            complement(4929..6050)
                     /locus_tag="VFB80_11560"
     CDS             complement(4929..6050)
                     /locus_tag="VFB80_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34452.1"
                     /translation="MVHVPTSTPPAATAPQPGRWRRFFRLSLRTLLLLVVLLAVWLGI
                     TFNRVRQQRAAVARIEQLGGSVQYLHELDPDGLTFPWRIDEPVSPGPPWLRRLLGPEF
                     FETVVAVELNQTRVTDADLRIIGKLRGVVVAQLNCTSVSDAGLYELRNWRKLRSLGLM
                     DTQVTGAGLRHLASMPELNNLVLEQTAVDDQGLARLGAHKGLTSLSLGQTRVTSRGVE
                     QLRSYPELSILSLHSTPTDDSAVPALVSLRKLDYLDVTGTNISVQGLLVLCNAWPRKA
                     VVGLYIDLSGQSNRKFTRDGSLWLQVVARACALDSEDRLKLIDFSWSNIQDEHLAGLH
                     GLKNVELIDLRGSEATPAGIAELRKALPHCEVLYEGDRN"
     gene            complement(6474..7817)
                     /locus_tag="VFB80_11565"
     CDS             complement(6474..7817)
                     /locus_tag="VFB80_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34453.1"
                     /translation="MKAAAFACFALLLAGAPLQAAETRPNIVFIMADDLGWADVAFHG
                     GSAATPHLDRLAKEGLELTQHYVAPVCSPTRTGLLTGRCWSRFGVTSPQNPRALPWET
                     VTLPRALKQAGYDTCLTGKWHLGSLPDQGPNHFGFDHSYGSLAGGVSPWNHHYKQGPF
                     TQTWHRNEQLIEEQGHVTDLIAAEAVQWIKSRGEKPFFLYVPFTAVHLPIKEPDEWLR
                     RVPAAIQGEVPRHYAACVMHLDDAVGRIVAELERQGRRENTLLVFTSDNGGSTAENND
                     LSYPDDHCPSGKLPGNNTPWRGKKGDLYEGGTRVATIVQWPGRIKPGKVDSPVQITDW
                     MPTLCSLAGYQPQQDLKWDGRDISQLLTSHQPLPERPLYAVAPGWRSRSLRWGSWKLI
                     LHAMAGDVQRAELFDLARDPAESKNLAEEQPEQLRKMLAKLDEVAAGDRSAAVAP"
     gene            7991..>8611
                     /locus_tag="VFB80_11570"
     CDS             7991..>8611
                     /locus_tag="VFB80_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34454.1"
                     /translation="MLRTSFHRALAAAVLLLLPAPLLAGGPPFLCLPLEGVTAANARA
                     CTDLLDSRLASKFNQAGWPRGVKVLERTGQWYAAFYMGSDVRLADVTAALKGSQFSVP
                     QDKLHFFGHVILEIDPQKASPKALLADLEAMNFVSVDESKAGKDLLLVTVDMPYPAEN
                     GSLKLESVGWDTFHRSDLASDQATRSESPASAKQLPSYGQIRDVVSK"
BASE COUNT         1447 a         2830 c         2860 g         1474 t
ORIGIN      
        1 ccgtaatcca aaacgttttt gggacgccag agacccccgt tttctatttc ttggcaactc
       61 gttcctaatc ctaatcgtaa tcctaatccc gccgccctct ccgctcccgg ccgctttgga
      121 gccgcgagat taagattagg attacgatta agattaggac cagcggccaa gacgacaccc
      181 tagccctctc ccaaggggag aggggatttg ggactcagcg gagcgggatt cagggcgggc
      241 tggggtgcgg gctgccgcgc gggtgcgggg gcggcgagcc aggtcagagc gtggccgagg
      301 tcgtcgagat aggaatagat gaccggcacg gccagcagcg tgagcaagag cgacagcgtc
      361 tgaccgccga cggtgagcac cgcgatcgag cggcgctctt ccgcaccggg gcccgttgcg
      421 atcagcagcg gcaacaggcc ggcgacgaac gcgatcgtgg tcatcaggat cggccgcaga
      481 cggtcgcggt tggcttgcag gatcgcctcc aaccgcggca ggccgcgctc gcggagcacg
      541 ttggtgtgat cgatctggag gatcgaggct ttcttcacga cgccgaacag caccagaatg
      601 cccagcgcgg aatagaggtt gagcgtctcg ccgccccaat acaggctgat caggccgaac
      661 ggcacggcca gcggcagcga gagcaggatc gtgaacggat gcacgaggtg ctcgtactgg
      721 gccgcgagca cgatgtacat gaagatgaac gacagggaga aggtccagac gaagtcggtc
      781 agtgtccgct cgagttcgcg tccgcggccc agcacgcgcg tgctgaagcc cgtcggcttg
      841 ccgatttcgt ccaccgcctg gttgagggcg gcgatccggc tggccaggcc gtagcccgga
      901 gcgacgttgg cccgcaccgc gaccatccgc tcgcgatcga gccggtcgat gcgggcggcc
      961 gcttcgccga agtcgaactg cacgacgttg tcgatccgcg tcagcaccgg cccggacgag
     1021 ctcgctcggc cgccgcgcgg gttggcccgc acgtagagct gcgagatcga ttcgatgttg
     1081 cggcggtcga ggccgatcag cctgagctcg acgtcgtagg cgtcgtccac cgtcgcgtcg
     1141 cgataacggc tgacgcggtc gtcgcccccg acggccacgc ggagggtctc ggcgatctcg
     1201 cggacctcga cgccgatcgc cgctgaccgc tcgcggtcga tattcacgag cagctcgggc
     1261 ttgtccagcc gcagggtcgt atcgacgtcc acgatgccgg ggatttcggc ggccctttgc
     1321 cgcactcttt cgctgaaatc gaacagggtg ttgatgtccg ggccggtgat cgagaagtcg
     1381 atatcgaccg gcgcgccttg ccgcagcgag gtgaggttgc ggacgctgcc gcggagggcg
     1441 ggggttttct tgagcagctg gcggacctcg gccatcttgt cgcgctgcga gaagttgccc
     1501 cgccaggcct cgcccgggtc tcccttgagc agcccccgcc accagcggcc gagcgagaac
     1561 gagcggccgc gaatgtccgt cagccgcacg tacatctcgc cgcgattgac gccgccgaag
     1621 ccgcgggtgc cgaccgtggc caggatcagc tcgactcccg gcatggcgcg gatttcggcc
     1681 tcgactttgg cgagcgcctc gtgcatcgag gccaggctcg cgccttcggg agcttgcagg
     1741 ctgacctcga actccgactc gtcaacgttg gtgggaatgt agtcctgctt gaccaggtcg
     1801 tagagccaat agttggacgc gaccgtcacg gcgcagagca gcatcacggc ccagcggaac
     1861 cgcatcgaca gccgcagcga ccacatatag aagacttcca gccagtgata gaagccgcgc
     1921 cgcgaggccg gattgccggc ggtggaggtg cgggccggcc gcagcagctt gctgcacatc
     1981 atcggcgtga gcgtgaagct gacgagcatc gagaccatga tcgctacggt ggcggtgatg
     2041 ccgaactgga acagcatccg ccccgtgacg ctcgacagga acgagaccgg caggaagacg
     2101 atgaccagcg agatcgtcgt ggccagtacg gccgggccga tttcgcgggt gcccgcgatc
     2161 gacgccgcga ccgggtccat ccccttctct tcgatgcagt gaaagacgtt ctccaagacg
     2221 acgatcgcat cgtcgatcac cacgcccacc atcagcacca gggccagcat cgtgacgttg
     2281 ttgagcgtga agccgaacag cttcatgaag gcgaacgtgg cgacgatcga ggtcgggatt
     2341 gcgacggcgg cgatgaacgt cgagcgccag gaccgcatga acgccagcac ggtcaggctg
     2401 gccaggatgc tgcccacgat caggtggcct tcgatttcgt gcagggcggc gaggatgtac
     2461 cgcgactggt cctggatcac ggtgacctcg acgtcgcgcg gcaacaggtc gtgcgaccgc
     2521 tccaggcgct gcttgacgcc ctcgatcacg cggacggtgt tttcgcccga ctgccgctgg
     2581 acctgcagca cgacggcggg ccgtccgtta aggcgggcca gggtgcggac ctctttgctg
     2641 gcatcgcggg cctcgcccag atcggagatt cgcacggccg tatttccaac ggtcgtcacg
     2701 accaggtcgg caaattcgcg ggcgtgcggc acgcggccca gcgtccgcag gttgcgctcg
     2761 cggaggcctt catcgacgcg gccgcctggt acttcggcgt tttgcagggc cagggcttcg
     2821 cggacttgca ggatcgacag gtgataagcg gccagccgcc gggcgtcgat gttgatctgc
     2881 accgcgcggt cggccgcgcc ggcgatctgc acctggccga cgccgggggc cgattcgatg
     2941 acgtttttca cgtagcggtc ggccagcaag tagagctcgc tcgccgagcg cggcccggag
     3001 accgccagcg tgatgatcgg cgacgaatcg gtgtcctgtt tctgcacgac cggcgggtcg
     3061 agacccggcg gcaggcggtt gcggacgctg gcaaccgcgt cgcggacatc ctgtaccgcg
     3121 gcgtccaggt tccgcgtcag gttgaacgtc accatcacca tcgacatgcc gtcgctggag
     3181 atcgaccgca attcgtcgat gcccgcgacc gtcgcgacgg cgtcctccag cacctgcgag
     3241 acttccgact cgacctccgt cgaagccgca cccggataga ccgtccgcac atagaccgac
     3301 ggcaaatcca tgttcgggta gcgatcgacg cccaggttcg gaaacgcgac cacgcccgcc
     3361 accaccagcg acgtgatgat catcagcgcg aacaccggcc ggcggacgca caactcggca
     3421 agccaataca ccgaatgtcg ctcctgtcga gaatcaactg gcgggttacc gctccacggg
     3481 tcgccgcact gtgtgcggca agggcggtgt tggctcgctg ccggcttcag ccccgcctcc
     3541 ttcgccgccg gtcgagtcgg gcggctcggt cgagaccggc gcggtctgcg gctgctcggg
     3601 cgttgccgag atcggttcga tcttcgccac gcggccctgc gcgccgttct tcaggatgac
     3661 gtcgcccggc ttgaggccgc cgacgatctc gattccgccc gcgccgcggt gggcggtctg
     3721 cacgatctgc tctttcgctg ttccgtccac gaccttccag actttttccg cgccggcgaa
     3781 ttcggtcacc gccgacgagg gaacaaccag cgactgcgcg gccggatcga tcaccacttc
     3841 ggcctcggca aacagccccg tccgcagcgt gccgccctgg ttgggaacct ctgcctcgaa
     3901 cacgagcgag cggctcattt catcgaccac ggggctcagc cgcgtgatcg acacggccag
     3961 cggcgcgggc agcgattcga tcgacagcgt gacttcctgg ccgagcgcca gccgctgggc
     4021 gtagcgctcg ggcatcgtgc cgcggaaccg cagcttgccg gcgcggacga gcgtcaccag
     4081 cggatccccg acctgcacga acgagccgag ggccacgtgc cgctcctgca cgaagccgtc
     4141 gaacggggcg tgaatgaccg tgtccgtcag tcgctgcttg gcgaccgaca actcggcggc
     4201 ccgcacgccg atcagggcga ttttttcgcc aacgccgttg agggcggcgg aatagcgggc
     4261 gttggccacg ccctcggcac cgatcgccgt gtcgagctcg tcctgcgtga cggcgttctt
     4321 cacgcgaatc tgccgcagcc gttcgacgcg ggcgagtgcc tcgttccaga cggcccgggc
     4381 ctcgcggacc ggtggagagt tctcgggagt cagcccttcg accgggtccg tgggcttcat
     4441 gcctagcgcc gcgcgggctt gcacgagctg cgcctcggcc agcgcgactt gcagcttgaa
     4501 ctcctcctgc tcgagcgacg ccagctttgt gccggccggc accgtgtcgc ccagatcgac
     4561 gaggatttcg gccacccggc cgcccacctt ggccccgacg atcgtcacct cgtcggcgat
     4621 caggctgccc tgggcacgca cgatcgttgg aaaggcccgc ggctgcaccg tcagcaactc
     4681 ggccttgagc acgggcaatt ccggagcctt cggcggggcc gccgcgccgt gcccgccgca
     4741 gccggcgagc gtcagtccgc ccagcagggc caggccggca aggggcaggg cagaaaacga
     4801 ggaaccgcgt tgccgcatcg gcaggtccag gcgggccagg gagggactcg cgactccatc
     4861 cgccaagcgg gggcatccca gccctatcgt ttataccact ctctgccgcg acttgcgaac
     4921 gtatggcctc aattcctgtc gccctcgtaa agcacttcgc agtggggcaa agctttccgc
     4981 aattcggcga tgcccgctgg cgtggcctcg ctgccgcgga ggtcgatcag ctccacattc
     5041 ttcaggccgt gcaggccagc gaggtgctcg tcctgaatgt tgctccagga gaaatcgata
     5101 agctttagcc ggtcctcact gtctagcgcg caggcccgcg ctacaacctg gagccacagg
     5161 gagccgtcgc gggtgaattt cctgttggac tggccactca ggtcgatgta aaggccgacg
     5221 acggcctttc gcggccaggc attgcaaagc acgagcagcc cctgtactga aatgttcgtg
     5281 cccgtgacgt ctagatagtc cagcttgcgt aaggaaacga gggctggcac ggctgagtcg
     5341 tcggtcggcg tggaatgcag cgagagaatg ctcaactccg ggtacgagcg caattgctcg
     5401 actccccggg aggtgacgcg agtttgcccg aggctcagag acgtcagacc cttgtgggcg
     5461 ccgagacggg ccagaccttg gtcgtcaacg gcggtttgtt ccaacactaa gttgttcaat
     5521 tcgggcatgc tggcgagatg gcgcagtccc gcgccggtga cttgcgtatc catcaggcct
     5581 agcgagcgta gtttccgcca gttgcggagt tcatacagtc ccgcgtccga gacgctcgtg
     5641 caattcagct gcgcgacaac cacgccgcgg agcttcccga ttatccgcaa gtcagcatcg
     5701 gtcacgcgcg tctggttgag ctccacggca acaaccgtct cgaagaactc cggacccagc
     5761 aaccttcgca gccagggcgg accgggagac acaggctcgt cgatccgcca ggggaatgtc
     5821 aagccgtcgg ggtccagctc gtggaggtat tgaacgcttc cgcctagctg ctcgatccgc
     5881 gctacggcgg ctcgctgctg gcggacgcgg ttgaaggtta tccccagcca gacggccagc
     5941 agcacgacca agagcagcag cgtccgcagg ctcaggcgaa agaacctgcg ccagcggcct
     6001 ggctgcggtg ctgttgcggc tggcggtgtg cttgtgggga cgtggaccat tcgatctgag
     6061 tctcgcccgt ctcggcgtga gacatccaac tgggggcagt ctcacgcaga gacgcggggc
     6121 cgcggagggg atggcggcga ttggaccgcc ctaagctctc ggcgacgtat ataaggataa
     6181 ctggtcagcg gcgagatttt ttcctcgccc tcgccgcgag tatcgcgctg caaccgattg
     6241 ccgcgaaatc acttccgacg cgctgccggc gactccaagg ggttagcgtt tgaagcgttt
     6301 gaaaccccgc ggggagtttt ccttatattc tcgccgcgcc ggcgatgggc gtcgatctcg
     6361 cccttcgacg gtccgcacca ccgggccggc tggggccaaa atggcagcct acgggccaaa
     6421 tggaactcta gtgcaaatta cgtaccaatg ttcagtattc ggctaagact cgatcagggc
     6481 gcgacggcag cgctgcggtc gccggcggcg acttcgtcca gcttggcgag catcttccgc
     6541 aactgctcgg gctgctcctc ggccaggttc tttgactcgg ccggatcccg ggccaggtcg
     6601 aacagctcgg cgcgctgaac gtcgccagcc atggcgtgga ggatcagctt ccacgagccc
     6661 cagcggagac tgcgcgagcg ccagccggga gcgaccgcgt acagcggccg ctcgggcagc
     6721 ggctggtggg acgtcagcag ttggctgatg tccctcccgt cccatttcag gtcctgctga
     6781 ggttggtaac cagccaggga gcagagtgtc ggcatccaat cggtgatctg cacggggctg
     6841 tccaccttgc cgggcttgat ccggccgggc cattgcacga tggtcgctac ccgcgtcccg
     6901 ccttcgtaca ggtccccctt cttgccgcgc cagggcgtgt tgttgccggg cagcttgccg
     6961 ctggggcagt ggtcgtcggg atagctcagg tcgttgttct cggccgtgct cccgccgttg
     7021 tcgctggtga agacgagcag cgtgttctcg cggcggccct gacgctcgag ctcggcgacg
     7081 atccggccga cggcgtcgtc gaggtgcatg acgcacgccg catagtgccg cggtacttcg
     7141 ccctggatcg cggccggcac gcgccgcagc cactcgtccg gctccttgat cggcaggtgg
     7201 accgccgtga acggcacata caagaagaac ggtttctcgc cgcgcgactt gatccactgc
     7261 accgcttcgg cggcgatcaa gtcggtcacg tgcccctgct cctcgatgag ctgctcgttg
     7321 cggtgccagg tctgcgtgaa cgggccctgc ttgtagtggt gattccaggg gctgacgccg
     7381 cccgccagcg agccgtacga atggtcgaag ccaaaatggt tcggcccctg gtcgggcagc
     7441 gagcccaagt gccatttgcc ggtcaggcag gtgtcgtagc cggcttgctt cagggcccgc
     7501 gggagcgtca cggtctccca cggcagtgcc cgcggattct gcggactggt gacgccgaac
     7561 cggctccagc agcggccggt caacaggccg gtccgcgtcg ggctgcacac cggagcgacg
     7621 tagtgctgcg tcagctccag cccctccttc gccagccgat cgaggtgcgg cgtcgccgcg
     7681 ctgccgccgt ggaaggccac gtccgcccac cccaagtcgt cggccatgat gaacacgatg
     7741 ttcggcctgg tctcggcggc ctggagggga gcgccggcca gcagcagggc aaagcaggca
     7801 aatgcagcag ctttcatgtc ggcatccggc gagaggtagg agcagccggc attttcagtc
     7861 ctccggttgc ccgctaaaaa caacttttta agttatcaaa tcgataatta acaagtttgt
     7921 cagttatctg gccggccgta acattcggat ggcgtcgaga tatcgctgct tctttccgga
     7981 ggatcgttcc atgttgcgaa ccagtttcca tcgcgcccta gccgctgccg tcctgctgct
     8041 gttgccggct ccgctgctgg ccggagggcc gcccttcttg tgcctgccgc tggagggagt
     8101 cacggcggcc aatgcccggg cctgcaccga cttgctggat tcgcggctgg ccagcaagtt
     8161 caatcaggcg ggctggccgc gcggcgtcaa ggtgctcgag cggaccggcc agtggtacgc
     8221 cgcgttctac atgggcagcg acgtgcggct cgccgacgtg acggccgccc tgaaaggcag
     8281 ccagttctcc gtgccgcagg acaagctgca cttcttcggg cacgtgatcc tggagatcga
     8341 tccccagaag gcatcgccca aggcgctgct cgcggacctg gaggccatga actttgtctc
     8401 ggtggacgag tccaaagccg gcaaggacct cctcctggtg acggtggaca tgccctatcc
     8461 cgccgaaaac ggcagcctga agctcgaatc cgtcggctgg gacacctttc accgcagcga
     8521 cctggcctcg gatcaagcca ccaggtccga gtcgccggcc agcgccaagc agctgccgag
     8581 ctatggccaa atccgcgacg tcgtctcgaa g
//
LOCUS       DASRJB010000439         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1814470, whole genome shotgun sequence.
ACCESSION   DASRJB010000439 DASRJB010000000
VERSION     DASRJB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814470"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1031
                     /locus_tag="VFB80_11575"
     CDS             360..1031
                     /locus_tag="VFB80_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN34455.1"
                     /translation="MELLIVEDDQALGKALHRGLCESGHACRWVQSGQRGFEEASSQK
                     FDAVVLDLMLPDLPGLDLLKRLRQTGVRTPVLMLTALGSVDDRVSGLKAGADDYLVKP
                     FAFPELLARLEAILRRASERPRTVLEVGLLALDLATRRVTRGGSEITLTPTEFSLLEF
                     LMRYAGQVVTRKMLCEHLWEADWEGVTNVVEVHINRLRGKVDKGFEEPLIQTIRGRGY
                     LLKAS"
     gene            complement(1010..1411)
                     /locus_tag="VFB80_11580"
     CDS             complement(1010..1411)
                     /locus_tag="VFB80_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34456.1"
                     /translation="MPFAYLRDPLFLGCFATYWLHKLLVANGLSNDWLRAYLNDLICL
                     PFWVPIMLWPLRRLGLRTHDGPPTAIELVVPLVIFAAVFEVWLPATSTFAGLAVPDPL
                     DVLCYAGGGLASALFWNWRYGTRAGYEAFSK"
     gene            complement(1414..2175)
                     /locus_tag="VFB80_11585"
     CDS             complement(1414..2175)
                     /locus_tag="VFB80_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HZN34457.1"
                     /translation="MSPDRKPFQFSVRQLLAVFLALCVALALVTQLWHVGFVLALLLG
                     GIAAGAWRRDWRLTAAASTALLIFVTTYAASWLQWGESSLHSEQPLIRMQLDSIDRQL
                     QEHFQRYGQIPASLDDVPALAGTTDIWGHPFHYRKVADVSGAYELASLGRDGKPGGVG
                     LDADFSLAAFASDLGKRVTLWQFTFETNASAPIFIGATTASLVAAGIWYSAQRQERPV
                     VQRMILSLVVTTIAAVIVSVFLAGVHVVLSQSSGH"
     gene            2386..5643
                     /locus_tag="VFB80_11590"
     CDS             2386..5643
                     /locus_tag="VFB80_11590"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN34458.1"
                     /translation="MCALIFQTAWLREFRLIFGATTPASAAVLAIFMGGLGLGNALLG
                     KRADGSPNPLQMYGRLEMAISVGAIVSPLLVQLVRGVYIAVGGQDTLGIFGASALRLL
                     LASLVIGLPTFLMGGTMPAAARAVTGADDDSRTSVGWLYGLNTLGAVLGALLSTFVLL
                     ERLGTRETLWAAAAINLCNGFIAWKLSQRWPAASLVVENERVQGSGFRVQERKEKGKK
                     AAVGPRPVPEEAAQDQREGKPVSPAVSPQWLYAAAGIVGFAFLLMEIVWYRMLGPILG
                     GTTFTFGLILAVALAGIGVGGALYPQLFGRRVPTLRDFALTCGWEALAMAIPLALGDR
                     IAILTLALQDLRYFGFAGQVAAWAVIAGIVVFPAAVVSGIQFPLLVALLGRGSRDVGR
                     HLGWAYAWNTVGAMLGSLAGGFGLLPLVGAVGAWHCVVILLAGLALALLAADYLAQRT
                     AQPAQRNTAQPAARNTMRMLHPLFAAALAAACLFAAGPTEVWRHSGIGAGRSRAPTGT
                     TLNEVRNWMNDKRRSIAWQADGAEVGVAISTRGGIAFLVNGKSDGNVLSDAATQIMFP
                     MVGAMLHPNPQESLVIGLGTGESAGWLASLSGMRQVDVVEIEPAIAFVAEQCAPLNHN
                     VLKHPKVRVIYSDARETLQTTRRQYDLIASEPSNPYRSGVASLYTVEFYDAVRKRLKP
                     QGLFVQWLQGYEVDVATVRIVITTLREVFPNVEIWATKPGDLVLICGAEPFDYDLARL
                     RQRLAEPAFREAVRVGWRTTDVEGVLAHFVANAKFAGSVSSGPDKDLRINTDNQNLLE
                     YGFARTVGTSPEFSVAQLREEARSQNLHRPLTIPAGVVWEQVEDQVVAFNAGIGDAPL
                     PPQMFAGDRGKRAEALSQYFHGNSPAVLAAWSSQPKSPQGTIESAALALAYAEAGDDK
                     ARPLIDSLRADAPVDAALLEAVLAFHKQQPAEAARLMEQTCLTLRKDPIVLPRLAEVL
                     LTLALRIADADTDQKDRKFARRMYDALAEPFCVYLHDEKRRLTRCVLARHLGAGATAQ
                     ALVDVEPFVPWDRDFLKMRWLAYRDTKHPLEIRARRDLETFSRWAPEQLVLPPPQ"
     gene            5679..>5902
                     /locus_tag="VFB80_11595"
     CDS             5679..>5902
                     /locus_tag="VFB80_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN34459.1"
                     /translation="MIDNGTAVAKVVSQVADLPWVWGTWERGTGATGWASVSILPSFL
                     SCEGTVMSPFRCRRAGFTLVELLVVIAIIGV"
BASE COUNT          997 a         1910 c         2017 g          978 t
ORIGIN      
        1 ctgaggaatg tggcgcaggg gccggatcga cgttgaatgc cggcggggag gccgtcgtca
       61 tgagccgcag atcgtcacgg gtgcgaacaa tcggccgcga ggcgggaggg agacgcggcg
      121 tgcgtgtggg tcagcaggag ctgattcgac aactttagac gggccgcccc aggagggaat
      181 acgtgactta ggttacatct cggtaaggct ggcttaagtc gtttcacggc ggcgattagc
      241 ggcgattcgc cagccggctg ccgaagctta ataaatcgca agaaaaccgc gtgcccatcg
      301 tcaagcggcg gacttcgggc gagaatcgaa ttttgcgaca gtctcggaga gcgagcgaca
      361 tggaactgct gatcgtcgaa gacgaccagg cgctgggcaa agcgctgcat cgcgggctgt
      421 gcgaatccgg gcacgcctgc cgctgggtgc agagcggcca gcgcggcttt gaagaagcca
      481 gcagccagaa attcgacgcg gtggtgctcg atttgatgct gccggatttg ccggggttgg
      541 acctgctcaa gcgcctgcgg cagacgggcg tgcggacgcc cgtgctgatg ctgacggcgc
      601 tggggtcggt ggatgaccgc gtgagcggcc tcaaggccgg ggccgacgat tatctcgtca
      661 agccgttcgc atttccggag ctgctggccc ggttggaggc gatcttgcgg cgggccagcg
      721 agcggccccg cacggtgctg gaggtcggcc tgctggcgct cgacctggcc acgcgccgcg
      781 tgacccgcgg cggttccgag atcacgctca cgcccaccga gttcagcctg ctcgaattcc
      841 tgatgcgcta cgccgggcag gtcgtgaccc gcaagatgct ctgcgaacac ctctgggagg
      901 ccgattggga gggcgtgacc aacgtcgtcg aggtccacat caaccggctg cggggcaaag
      961 tcgataaggg cttcgaggag ccgctgattc agaccatccg cggccgcggc tatttgctga
     1021 aggcctcgta gccagctctg gtcccgtagc gccaattcca gaacagcgcg ctggctagcc
     1081 cgccgccggc atagcacagc acgtcgagcg gatcgggcac ggccagtccg gcaaaggtgc
     1141 tcgtcgcggg cagccagacc tcgaacacgg cggcaaagat caccagcggc acgaccagct
     1201 cgatcgccgt aggcggcccg tcatgcgtcc gcaggcccag tcgccgcagc ggccagagca
     1261 tgatcggcac ccagaacggc aggcagatca ggtcgttcag gtaagcccgc agccaatcgt
     1321 tgctcaggcc gttggcgacg agcagcttgt gcagccagta cgtcgcaaaa cagcccagga
     1381 acagcggatc gcgcaagtaa gcaaaaggca cggctagtgt ccggaggatt gcgagaggac
     1441 gacgtgtacg cccgccagga agaccgacac gatgacggcc gcgatggtgg tcacgactag
     1501 actgaggatc atgcgctgga ccacagggcg ctcctgtcgt tgcgcggagt accagatgcc
     1561 cgcggccacc agactggcgg tcgtcgctcc gataaagatc ggagcgctgg catttgtctc
     1621 gaaggtaaac tgccacagcg tcacccgttt tcccaggtct gaggcgaacg cagccagaga
     1681 gaaatcggcg tccaggccaa cgccgcctgg cttgccgtca cgcccgagcg aggccaactc
     1741 ataagcgccg ctcacgtcag cgaccttccg gtagtgaaag ggatgtcccc agatgtcggt
     1801 cgttccggcc agcgctggca cgtcatcgag cgatgcggga atctgtccgt aacgctgaaa
     1861 gtgttcctgc aattggcggt cgattgaatc caattgcata cggatgagag gctgctcgct
     1921 gtgcagcgat gactcgcccc actgcagcca actggcggcg tatgtcgtga cgaaaatcag
     1981 cagggccgtg ctggccgcgg ccgtcagccg ccagtcgcgc cgccaggcgc ctgcggcgat
     2041 cccgccgagc aatagcgcca gcacaaatcc cacgtgccac agttgcgtga ccagggccag
     2101 cgcgacgcac agcgcaagga atacggccag cagctgccgg acggagaact gaaacggctt
     2161 tcggtcgggg gacattgcca ggaattctca ccagccggcc ggcctctggc aatgaacgca
     2221 gctgcttcgg ccgtttcaca ctttcttaac cccgcccggg catcgccaag gggcggaaaa
     2281 catgcgaaaa cctacggctc ggaaacctgc cgaaaagccc attcgtcccg ctcgcgcatg
     2341 aactcccgcg tccgcacggc tgcgctgttg ctcttcgtat cgggcatgtg tgccctcatt
     2401 ttccagacgg cctggctgcg cgagttccgg ctgatcttcg gcgcgacgac ccccgcgtcc
     2461 gccgccgtgc tggcgatctt catgggcggg ctgggcctgg gcaatgcgct gctcggcaag
     2521 cgggccgatg gcagcccgaa tccgctgcaa atgtacggcc ggctggagat ggcgatcagc
     2581 gtcggcgcta tcgtcagccc gctcttggtg caactggtcc gcggagtcta catcgccgtc
     2641 ggcgggcagg acacgctcgg catcttcggc gcgagcgccc tccggctgct gctggctagc
     2701 ctcgtaatcg gcctgccgac gttcctgatg ggcggcacga tgccggccgc tgcgcgggcc
     2761 gtgaccggtg ccgacgacga cagccgcacc agcgtcggct ggctctacgg gctgaacacg
     2821 ctcggcgcgg tgctcggcgc gctgctcagc acgttcgtgc tgctcgagcg gctgggcacg
     2881 cgcgaaacgc tgtgggccgc cgcggcgatc aatctctgca acggattcat cgcctggaag
     2941 ctgtcgcagc gctggccggc ggcttcgctc gtcgtcgaga acgagagggt tcagggttcg
     3001 gggttcaggg ttcaggagcg gaaagagaag ggcaagaagg cggccgtggg cccgcgccca
     3061 gttccggagg aggcagccca ggatcagcgg gaaggcaagc ccgtcagccc ggcagtttcc
     3121 ccgcagtggc tctatgccgc cgccggaatc gtcggcttcg cgttcctgct gatggagatc
     3181 gtctggtatc gaatgctcgg gccgatcctg ggcgggacaa cgtttacctt cggcctgatc
     3241 ctggcggtgg cgctcgcggg catcggcgtc ggcggcgctt tgtatccgca gctgtttggc
     3301 cgccgcgtgc cgacgctccg cgacttcgcg ctcacttgcg gctgggaagc gctggcgatg
     3361 gctatcccgc tggccttggg cgaccgcatt gcgatcctga cgctcgcgct tcaggacctg
     3421 cgctatttcg gcttcgcagg gcaagtcgcg gcctgggcgg tgatcgccgg aatcgtcgtt
     3481 tttcccgcgg cagtcgtcag cggcattcag tttccgctgc tcgtcgccct gctggggcgc
     3541 ggcagccgcg acgtgggccg gcacttgggc tgggcctacg cctggaacac ggtcggggcg
     3601 atgctcggct cgctggccgg cggattcggc ttgctgccgc ttgtcggcgc ggtcggcgcc
     3661 tggcactgcg tcgtgattct gctcgcgggt ctggccctgg cgctgctcgc cgccgactat
     3721 ctggcccaac ggacagcgca gcccgcccag agaaacacag cgcagcccgc cgcaagaaac
     3781 acaatgcgga tgctgcaccc gctgttcgcg gccgccctgg cggccgcgtg cctgttcgcc
     3841 gccggcccga cggaagtctg gcggcactcc ggcatcggcg cgggccgctc gcgcgcgccg
     3901 accggcacga cgctcaacga agtcagaaac tggatgaacg acaagcggcg gagcatcgcc
     3961 tggcaggccg acggcgcgga agtcggcgtg gcgatctcga cccgcggcgg catcgcgttt
     4021 ttggtcaacg gcaagagcga cggcaacgtg ctttccgacg ccgccacgca gatcatgttt
     4081 cccatggtcg gggcgatgct gcatcccaat ccgcaggaga gcctggtcat cggcctgggc
     4141 accggcgaat cggccgggtg gctggcttcg ctgagcggca tgcggcaggt cgatgtcgtg
     4201 gagatcgagc cggcgatcgc cttcgtcgcc gagcaatgcg ccccgctcaa ccacaacgtc
     4261 ctcaaacatc ccaaggtgcg ggtgatctat agcgacgccc gcgagacgct gcagaccacg
     4321 cgccgccagt acgacctcat cgcgtccgaa ccgagcaatc cctaccgctc gggcgtcgcc
     4381 agcctgtata ccgtggagtt ctatgacgcg gtgcgaaagc gactgaagcc ccagggcctg
     4441 ttcgtgcaat ggctccaggg ttacgaggtc gatgtggcga ccgtgcggat cgtgatcacg
     4501 acgctgcggg aagttttccc gaacgtcgag atctgggcca ccaagccggg cgacctggtg
     4561 ctgatctgcg gcgccgagcc attcgactac gacctcgccc ggctgcggca gcggctggcc
     4621 gagcccgcct tccgcgaagc cgtgcgggtc ggctggcgga cgacggacgt ggagggcgtg
     4681 ctggcccatt tcgtggccaa cgccaaattt gccggcagcg tctcgtccgg gccggacaag
     4741 gacctgcgga tcaacaccga caaccagaac ctgctggaat acggtttcgc ccgcaccgtc
     4801 ggcaccagcc ccgagttttc ggtagcgcag ctgcgcgagg aggcccgctc gcagaacctg
     4861 caccgccccc tgacgattcc cgcgggagtc gtttgggaac aggtcgagga ccaggtcgtg
     4921 gcgtttaacg ccggcatcgg cgatgcgccc ctgcctccgc agatgtttgc cggcgaccgc
     4981 ggcaagcggg ccgaagccct gagtcagtat ttccacggca attcgcccgc cgtgctggcg
     5041 gcctggtcga gccagcccaa atcgccccag ggcacgatcg agtccgccgc gctggcgctg
     5101 gcctacgccg aagcaggcga cgacaaggcc cgcccgttga tcgattcgct gcgggccgac
     5161 gcaccggtgg atgccgcgct cctggaggcc gtcctggcgt tccacaagca gcagccggcg
     5221 gaggccgccc ggctgatgga gcagacgtgc ctcacgctgc ggaaggaccc gatcgtcctg
     5281 ccgcggctgg cggaagtgct ccttacgctc gctctgcgga tcgccgatgc cgacacggac
     5341 cagaaggatc ggaaattcgc cagacggatg tacgacgcgc tggcggagcc gttctgcgtc
     5401 tacctgcacg acgagaagcg gcggctgacg cgctgcgttc tggcccgcca cctcggtgcg
     5461 ggggcgaccg cccaagcgct ggttgacgtg gagcccttcg tgccgtggga ccgcgacttt
     5521 ctgaaaatgc gctggctggc ctaccgcgac acgaagcatc ctctggaaat ccgggcccgc
     5581 cgggacctgg agaccttcag ccgttgggcg ccggagcaac tggtcctgcc gccgccgcag
     5641 taggcaagcg tgcccgaggg cgataaaaac ccccaccgtt gattgacaac ggcaccgcag
     5701 ttgctaaagt cgtgagtcag gtcgcagatt tgccttgggt ttggggaact tgggaacggg
     5761 gaacgggcgc gaccggttgg gcgtcggtgt cgattctccc atcgttcctt tcttgcgagg
     5821 ggaccgtcat gtctccgttc cgctgtcggc gcgcgggttt cactctggtc gaactgctcg
     5881 tggtgatcgc catcattggc gt
//
LOCUS       DASRJB010000440         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_684913, whole genome shotgun sequence.
ACCESSION   DASRJB010000440 DASRJB010000000
VERSION     DASRJB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684913"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFB80_11600"
     CDS             complement(<1..627)
                     /locus_tag="VFB80_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34460.1"
                     /translation="MPEEGTIVDSARQRAFRVPLDHFRRADGLFLVKLWLSLAAAGLA
                     AAYVAWLLLDSRAGGRHASPGALTAAHASWNDQCRSCHVDFRPLAGGAADLFAPAAER
                     SPAAHRQFLDRACIECHPRQAAPHHANQPEAEVPSCAACHAEHHGATADIRRPADEEC
                     TSCHADIGGHRAESKYILPIGNASRFDAAAHPAFRSLRSDPGNIKFNHW"
     gene            complement(639..1607)
                     /locus_tag="VFB80_11605"
     CDS             complement(639..1607)
                     /locus_tag="VFB80_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34461.1"
                     /translation="MLIDRRGFWKLHGSWLALSVVLAAAAIGWTVLEARALGRWPGGG
                     SLPGLVLGTAAAAIFLFEAGLILKKTRLLRTARWTLSAQTWMKAHIWLGLLTVPLVVL
                     HSGGHLGGILTTLLVVVFGVVIASGLWGLALQNLLPRMLLEAAPAETIYSQIESVGRQ
                     YADQARRLVLVACGGEPIEEPALQPAGAADREIRGAPRPVGPLIKRSPHPAQGVQGQV
                     DSPALRAALSETIEPFLAAGICRQGLLGSRQRNQWFFDDLRLRAPPELRELVGQLEDL
                     CERRRQLNVQRRLHFWLYNWLWLHWPLSAALLILLAGHVVLALRFG"
     gene            complement(1659..2948)
                     /locus_tag="VFB80_11610"
     CDS             complement(1659..2948)
                     /locus_tag="VFB80_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34462.1"
                     /translation="MRQLIYWSAAAGLLTALLVGSQSAGQELAAGEAPRYFFDPYVEP
                     AERLQPPAESITGEAPRQPTRRTRSQQANIRLASVPNMFGDLAMLTGIATVRIPNQVG
                     NNTIVASQFGIPTVGSLRSGKISENDTPIPVDRVFFNYNHFHNLFAVNEQQLFPPGQP
                     PIARQESLDRYTVGAEKTFLDGLFSIELRMPFFGAPDVEQQTFGIQNGNYGNLTAVLK
                     ALLYSDDSLAVGAGVAVCTPTGSDTVARIGLASLRFENEAVHLLPYLGFIYSPGDPTW
                     GWGDGLFLTGYVQFDAAANSNPVEVRVLDGPLVGTAGKFTEQNLLFFDIGVGYWLLRN
                     PYAPRVTGLAAVAEFHYTTAVQDADVLATSVGPGVGAVTLTNPFNRFDVVNFTAGVQA
                     LLFDASSLRVAFATPLGSGEDQRLFDAEVIVQYNLRF"
     gene            3112..4197
                     /locus_tag="VFB80_11615"
     CDS             3112..4197
                     /locus_tag="VFB80_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZN34463.1"
                     /translation="MAEIPPNRFVGRGTSLAPPNRFEQVRSEADVDQLAAGDELLAAD
                     RRTPTVFLPDHAASIIRENDSPDIPFRYSMNPYRGCEHGCAYCYARPTHETLGLNAGL
                     DFETKVLVKHDAPALLRKELNRKSWQCEPIMISGVTDCYQPAERTYQLTRGLLEVLLE
                     AHQPACIITKNALILRDLDLLAAMAEKNLVSVALSTTTLDAELARVLEPRTATPAARF
                     RAIRELAGAKVPVRAMLAPIIPGLTDNEIPALLTAAKEAGAWGAGYVMLRLPLAVAPI
                     FTAWLAEHRPQAAARVEGLIRQTRGGRLYKSEWGERMSGSGNYAESIAQTFEVFSQKL
                     GFDQKWQELDCSQFRPPELTAGQLRLF"
     gene            4286..5737
                     /locus_tag="VFB80_11620"
     CDS             4286..5737
                     /locus_tag="VFB80_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34464.1"
                     /translation="MSLANRRAFLADVGMGFTGLALGAMLARDGVAASESHREADASR
                     SPGPAKSVIWIFLVGGMSQMESFDPKPELTKYAGKTIGESPYKDTLESPHLKKNLREV
                     IEGLHKVHPKIYELQVGFQKYGQAGLEISDWWRTLGEWCADDLCLVRSMWTTDNNHGA
                     QLQFHTGRHSLEGQYPTIGSWVHYGLGSLNDNLPQFVVLGTPIADCCGGAGAHGANYL
                     GPRHNGIQLVVDPRNPLPFAAPDKSVYREEQAREFELLGKLNRLAAVEYPDDPALAAR
                     IRSYELAFEMQSAVPDVVNFAAETAETQQLYGLDQPATKEFGSQCLAARRMVERGVRF
                     IQLFHGSNGGAGAWDAHANLKHGHTKLCGQIDRPIAALIHDLKRRGLLNETLVVIGTE
                     FGRTPGAQGADGRDHHPYGFSVALAGGGIRGGLAHGATDELGFHAVENRHYVTDLHAT
                     LLHQLGLEARQLEIPGRKRLEVDFGTPIRPIVA"
     gene            complement(5734..>8426)
                     /locus_tag="VFB80_11625"
     CDS             complement(5734..>8426)
                     /locus_tag="VFB80_11625"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN34465.1"
                     /translation="LAAAQVLAQSLWWFHPLVWLANRLLSREAERCCDEQVLAELGCE
                     PARYARSLLDILQLKHELLAVPAFPGVRPVDVTSNRLERIMRLGHGCQRRTPWWCWLV
                     MLLAAAIVLPGAAFVAGAKERPGKLKQIGWKQSAAAATQATPATPAEGTGERVTRVYP
                     ARDLLARLMEELRVDEAQAREDLRGHVIGLAQLQDPQLAWEGKKLVATHSAAQHERVA
                     AALAQLREFGVRQITLSVRMVSGPPALLERFPIRWSAADTALAEPGVRPTSQVFAALD
                     FEPLPLPAEAKPGESWTQAASVIEKNMPVLYAVLTAEQARPLIETAQGDSRANILQSP
                     KVTVFNGQRATVMDATQRPFVVGVKPVASDDPSAPPAFEPQIRVVSEGTTLRMRPVLQ
                     ARDQLRLDYEFTLASVRSVETAKIPQGKGAEPLTVQVPEVASVRFCSSLQMPLGRTLA
                     VGVLTKQGAKPGAMVVLLEVNDIKPAGDHQAAPVAAKDSPTLPAPRAVAGASHLTAAE
                     AALEERLQRKIFAAHGRMTLDQAIEAISKAAGINILIDQRGLAAEGVTLDTPVQVQRQ
                     GVAVAAGTLLRQLLEPLEMAFVIENECLRVTGASELRKPFTKVYLVADLIAAVPQIVG
                     VVDGKPLPPPAVAKADFDPLIQLITTTIAPQSWDSTGGPGSLSAFETNMSLVVSQSQE
                     VHEQIADTLQQLRRLRDQQVVLELQLLCVEKTFFERLGLDLVIEPADDKASIARARLT
                     AQQAASLLRAVGPAPRGSSRLTLLNGQAGDLDLPLGGKERKVNLKLVPRIRFDARSIL
                     LELAVGSEEGEDAAAKSVDLICDAGQVLLLDVTDRIPAAGVPMLTKLPHVGPLFKQTP
                     PAGSRVLLLVTPEIHFGEEVVVGEASRAAATHAAASAKQH"
BASE COUNT         1393 a         2862 c         2850 g         1321 t
ORIGIN      
        1 ccaatgattg aacttgatgt ttccagggtc ggatcgcagc gagcggaatg cgggatgagc
       61 agccgcgtcg aagcgggagg cattcccgat cggcagaatg tacttagatt ccgcgcggtg
      121 cccgccgatg tccgcgtggc agctggtgca ttcttcgtcg gcgggtcgcc ggatgtcggc
      181 cgtcgcgccg tggtgctctg catggcacgc ggcgcaactg ggcacttccg cttccggttg
      241 attcgcgtga tgtggcgctg cctgccgcgg atggcattcg atgcaggctc gatcgaggaa
      301 ctggcgatgc gccgctggcg agcgctcggc ggcgggcgcg aacaaatcgg ccgcgccgcc
      361 ggcgagcggt cggaaatcga cgtggcagct gcggcactgg tcgttccatg aggcgtgcgc
      421 ggcggtgagc gctccgggcg aagcgtgccg gccgccggcc cggctatcga gcagcagcca
      481 cgcgacgtac gccgcggcga gcccggcggc cgcgagcgac agccacagtt tgacgaggaa
      541 gagcccgtcc gcgcggcgga agtggtcgag cggcacgcgg aaggcccgct gcctggccga
      601 atcgacgatg gttccttcct ccggcatcgt gcccccgctc agccgaatcg taacgccagg
      661 accacgtgcc cggcgagcaa aatgagcagc gccgccgaca gcggccagtg cagccagagc
      721 cagttgtaca gccagaagtg cagccgccgc tgcacgttga gctgccgccg ccgctcgcac
      781 aggtcctcca gctggccgac cagctcgcgc agctcgggcg gcgctcgcag ccgcaggtcg
      841 tcgaagaacc actggttccg ctgccgcgag ccgagcagcc cctgccggca gatgccggcc
      901 gccaggaacg gctcgatcgt ctcgctgagg gcggcccgca gggcgggcga atcgacctgg
      961 ccctgcacgc cctgggccgg atgcggcgaa cgcttgatga gcggtccgac cggacgcggt
     1021 gcgccgcgaa tctcgcggtc tgcagcgccg gccggctgca gcgccggctc ttcgatcggt
     1081 tcgccgccgc aggcgacgag caccagccgg cgggcctggt cggcgtattg ccggccgacg
     1141 ctctcgatct gcgaatagat cgtctcggcc ggcgcggcct ccagcagcat ccgcggcagc
     1201 aggttttgca gcgccagacc ccacaggccg ctggcgatga cgacgccgaa gaccacgacg
     1261 agcaaggtcg tcagaattcc gcccaagtgg ccgccgctgt gcagcacgac cagcggcacg
     1321 gtcaagaggc cgagccagat atgcgctttc atccaggtct gggccgagag cgtccagcgg
     1381 gccgtccgca acagccgcgt cttcttaagt atcagcccgg cttcaaatag aaagatcgcg
     1441 gccgcggccg tgcccagcac cagacctggc aggctgccgc cgccgggcca gcggccaagc
     1501 gctcgggcct caaggactgt ccagccgatt gcggcagccg cgagcaccac gctcagcgcc
     1561 agccagctgc catgaagttt ccagaaaccg cgtcgatcga tcagcacgcc gggccgtcct
     1621 ttggcctgat cgtatcaact tggtcttttg ccggcgagtc agaaacgcag gttgtactgc
     1681 acgatgactt cggcgtcgaa cagccgctgg tcttcgcccg agccgagcgg cgtcgcaaag
     1741 gcaacccgca ggctcgacgc atcgaagagc agcgcctgca cgccggcggt gaagtttacg
     1801 acgtcgaagc ggttgaacgg attcgtcagg gttacggcgc cgacgccggg gccgacgctc
     1861 gtcgccagca cgtcggcgtc ttgcaccgcg gtcgtgtagt gaaactcggc cacggctgcc
     1921 aggcccgtca cccgcggggc gtacggattg cgcaatagcc agtagccgac gccgatgtcg
     1981 aagaagagca ggttttgctc ggtgaatttc cccgccgtgc cgaccagtgg tccgtcgagg
     2041 actcgaacct ccacgggatt gctgttggcg gcggcgtcga actgaacgta gccggtcagg
     2101 aacagcccgt cgccccagcc ccaggtcggg tcgccgggcg aatagatgaa gccgaggtac
     2161 ggcagcaggt gcaccgcttc gttctcgaac cgcaggctgg ccagtccgat gcgagccacc
     2221 gtgtcgctgc ccgtcggcgt gcagacggcg actcccgcgc ccacggccag cgagtcgtcc
     2281 gagtaaagca gggccttgag gaccgcggtc aggttgccgt agttgccgtt ctggatgccg
     2341 aaagtctgct gctcgacgtc gggcgcgccg aaaaacggca ttcgcagctc gatcgagaac
     2401 agcccgtcca ggaacgtctt ctccgcgccc acggtgtacc ggtcgagtga ctcttgccga
     2461 gcaatgggag gctggcccgg cggaaaaagc tgctgctcgt tgacagcgaa cagattgtgg
     2521 aagtggttgt agttgaagaa cacgcggtcc acgggaatcg gcgtgtcgtt ctccgagatc
     2581 ttgccgctgc gcaggctgcc cacggttgga atgccgaact ggctggcgac gatcgtgttg
     2641 ttgccgacct ggttcgggat ccggacggtc gcgatgccgg tcagcatcgc caggtcgccg
     2701 aacatattcg gcacgctcgc caggcggatg ttcgcctgct gcgaccgcgt gcggcgggtt
     2761 ggctgccggg gcgcctcgcc ggtgatcgac tcggccgggg gctgcagacg ctcggcgggc
     2821 tcgacgtacg gatcgaagaa gtagcgcggc gcttccccgg ccgccagttc ctggcccgcc
     2881 gattggctgc cgaccagcag ggccgtcagc aggcccgccg ccgcgctcca gtagatgagt
     2941 tgccgcattc gcccccctgc gatgaactgc gccgtatccg ttggtatcgg ccaggccgcc
     3001 ggtatgggtg aaggcggatt tggcgattat tctgccggcg cggctgctag cggccgccta
     3061 gaccattttg atgataactg tataataacc cacatcaggg ccttcccgcc gatggcagag
     3121 attccgccaa accgcttcgt gggccgaggc accagcctcg cgccgcccaa ccgcttcgag
     3181 caagtgcgga gcgaggccga cgtcgatcaa ttggcggccg gcgacgaact gctcgccgct
     3241 gaccgccgca cgcccaccgt cttcctgccc gaccacgccg cctcgatcat ccgcgaaaac
     3301 gacagcccgg atatcccctt ccgctacagc atgaacccct accgcggctg cgagcacggc
     3361 tgcgcgtatt gctacgcccg cccgacgcac gaaacgctgg ggctgaacgc gggcctcgat
     3421 ttcgagacca aggtgctcgt caagcacgac gcgccggcgc tcttgcggaa agagctgaac
     3481 cgcaagagct ggcagtgcga gccgatcatg atctcgggcg tcaccgactg ctatcagccg
     3541 gcggaacgca cctaccagct gacccgcggc cttctcgagg tgctgctgga ggcgcatcag
     3601 ccggcgtgca tcatcacgaa gaacgcgctg atcttgcggg acctggactt gctggcggcg
     3661 atggccgaga agaacctggt cagcgtcgcc ctcagcacga cgacgctcga tgccgaactg
     3721 gcccgcgtgc tggagccgcg gacggcaacg ccggcggccc gctttcgcgc cattcgcgag
     3781 ctagcggggg ccaaggtgcc cgtgcgggcg atgctcgcgc cgatcattcc cggactgacc
     3841 gacaacgaga ttcccgcact ccttacagcg gccaaggagg ccggcgcttg gggagccggc
     3901 tacgtcatgc tgcggctgcc gctggcggtg gccccgatct tcacggcctg gctcgccgag
     3961 caccgcccgc aggctgcggc tcgggtagaa gggctgatcc gccagacgcg cggcgggcgg
     4021 ctctacaaat ccgagtgggg cgagcggatg agcggcagcg gcaactacgc cgagagcatc
     4081 gcccagacct tcgaggtgtt ttcgcagaag ctcggcttcg accagaagtg gcaggaactc
     4141 gactgctcgc agttccgccc gcccgagctg accgcggggc agctgcggct gttctaggtc
     4201 tgcccctcca caactcggtt gcagccatcg gcgacctgag ggagaatcaa ttcctccccc
     4261 catcactgcc cgccaggatt ccagcatgag cctcgccaac cgccgcgcgt ttctggccga
     4321 cgtgggcatg ggtttcaccg ggctggcgct gggggccatg ctggcgcgtg atggggtcgc
     4381 tgcgagtgaa agtcatcggg aggctgacgc ctcccgctcg ccggggccgg cgaagtcggt
     4441 catctggatt ttcctggtcg gcgggatgag ccagatggag agtttcgatc ccaagccgga
     4501 gctgacgaag tacgccggca aaacgatcgg cgagtctccg tacaaggata cgctggagtc
     4561 gccccacctg aagaagaacc tccgcgaggt gatcgaaggg ctgcacaagg tccacccgaa
     4621 aatctacgag ctgcaggtcg gttttcagaa atacggccag gcgggcctgg agatcagcga
     4681 ctggtggcgg acgctgggcg agtggtgcgc ggacgacctg tgcctggtcc gcagcatgtg
     4741 gacgactgac aacaaccacg gcgctcagct gcagttccac accggccgcc attcgctcga
     4801 agggcagtac ccgaccatcg gctcctgggt ccactacggc ctcggttcgc tgaacgacaa
     4861 cctgccgcag ttcgtcgtgc tgggcacgcc gatcgccgat tgctgcggcg gcgctggagc
     4921 gcacggcgcg aactacctcg gcccgcggca caacggcatt cagctcgtcg tcgatcccag
     4981 aaatccgctg ccgttcgccg cgccggacaa gtcggtctat cgcgaggagc aggcccgcga
     5041 gttcgaattg ctgggcaagc tcaaccggct ggcagcggtc gaatatccgg atgaccccgc
     5101 gctggccgcc cggatccgct cgtacgagct ggcgttcgag atgcagtcgg cggtgcccga
     5161 cgtcgtgaat ttcgcagccg agacggccga gacgcagcag ctctacggcc tcgatcagcc
     5221 ggcgacgaaa gagttcggct cgcagtgcct ggcggcgcgg cggatggtcg agcggggcgt
     5281 gcggttcatc cagctcttcc acggcagcaa cggcggcgcg ggcgcgtggg acgcccacgc
     5341 caacctgaag cacggccata caaagctgtg cggccagatc gaccggccga tcgccgccct
     5401 gatccacgac ctgaagcgcc gcggcctctt gaacgagacg ctcgtcgtca tcggcaccga
     5461 gttcggccgc acaccgggcg cgcaaggcgc cgacggccgc gaccatcatc cctacggctt
     5521 ctcggtcgcc ctggccggcg gcggcatccg cggcggcctg gcccacggag ccaccgacga
     5581 gctgggcttc cacgcggtgg agaaccgcca ctacgtcacc gacctgcacg ccacgctgct
     5641 ccaccaactc ggcctcgaag cgcgccagct cgaaatcccc ggccgcaagc ggctcgaagt
     5701 ggacttcggc acgccgatcc gcccgatcgt ggcctagtgt tgcttcgcgg acgccgcggc
     5761 gtgcgtcgcc gcggcgcggc tggcttcgcc gaccacgacc tcctcgccga agtgaatctc
     5821 cggcgtcacc agcagcagca cgcggctgcc ggcgggcggc gtttgcttga agagcgggcc
     5881 gacgtgcggc agcttcgtga gcatcggcac gccggccgca ggaatgcggt cggtcacgtc
     5941 gagcagcagg acctggccgg catcgcagat gaggtccacg ctcttggccg cagcgtcctc
     6001 gccctcctcg gagccgacgg ccagctccag gaggatcgag cgggcgtcga accgaatcct
     6061 cggaacgagc ttcaagttga ccttccgctc cttgccgccc aacggcaggt cgagatcgcc
     6121 cgcttgcccg ttcagcagcg tcagccggct gctgccgcgc ggcgccggtc cgacggcgcg
     6181 gagcagcgac gccgcctgct gcgccgtcaa ccgcgcccga gcgatcgacg ctttgtcgtc
     6241 cgctggttcg ataacgagat cgagtcccag ccgctcgaag aaggtcttct cgacgcaaag
     6301 cagctgcagc tccagcacga cctgctgatc gcgcaggcgg cgcagctgct ggagcgtatc
     6361 ggcgatttgc tcatgcactt cctgcgattg cgagacgacg aggctcatat tggtttcgaa
     6421 tgccgaaagc gatcccggac cgccggtcga gtcccacgac tgcggcgcga tcgtggtcgt
     6481 gatcagctgg atcagtggat cgaagtccgc cttggcgact gccggcggcg gcaggggctt
     6541 tccatcgacg acgccaacga tttgcgggac ggcggcgatg agatcggcga cgagatagac
     6601 cttcgtgaat ggcttgcgaa gttcgcttgc gccagtcacg cggagacatt cattctcgat
     6661 gacgaacgcc atttccagcg gctccagcaa ctgcctgagc agcgtcccgg cggcaaccgc
     6721 gacgccttgc cgctgcacct ggacgggggt gtccagcgtg accccctctg cggccagccc
     6781 gcgctggtcg atcagaatgt tgatgccagc cgccttgctg atcgcctcga tcgcctggtc
     6841 cagcgtcatc cggccgtgag cggcgaagat ctttcgctgc agccgctctt cgagcgcggc
     6901 ttcggccgcc gtgagatggc tggccccagc gacggcgcgg ggagcgggca aggtcgggct
     6961 gtccttcgca gcaacgggag cggcctgatg gtcgcctgcc ggtttgatgt cattgacttc
     7021 caagagcacg accatcgcgc ccggcttggc gccctgtttg gtgagcacgc cgacggcaag
     7081 cgtccggccc agcggcatct ggagcgacga gcagaagcgg accgaggcga cttcgggaac
     7141 ctggacggtg agcggctccg cacctttccc ctgcggaatc ttcgccgtct cgacgctgcg
     7201 gacgctggcc agcgtgaact catagtccag ccgcagctga tcgcgggcct gcagcaccgg
     7261 ccgcatccgc agcgtcgtgc cttcgctgac gacgcgaatc tgcggctcaa aggcgggcgg
     7321 cgcactcgga tcgtcactcg ccaccggctt gacgccgacg acgaagggcc gctgcgtggc
     7381 gtccatgacg gttgcccgct gcccgttaaa gacggtgacc ttgggcgatt gcaaaatatt
     7441 cgcacggctg tcgccctgag cggtttcgat gagcggccgg gcttgctctg cggtgaggac
     7501 ggcgtagagc accggcatgt tcttctcgat cacgctcgcg gcctgcgtcc acgactcgcc
     7561 cggtttggct tccgccggca gcggcagcgg ctcaaaatcc agcgcggcaa acacctggct
     7621 ggttggcctg actcccggtt ctgccagcgc ggtgtcggcg gccgaccagc gaatggggaa
     7681 tcgctccagc agcgccggcg gaccggagac catgcggacg ctgagcgtaa tttgccgcac
     7741 gccaaattcg cgcagctgcg cgagggctgc ggcgacgcgc tcgtgctggg ccgcgctgtg
     7801 cgtggcaacg agcttcttgc cctcccaggc cagttgcggg tcctggagct gggccaagcc
     7861 gatgacgtgc ccgcgcaggt cctcgcgggc ctgggcttca tcgaccctca actcctccat
     7921 caaacgggcc agcagatcgc gagccggata gacccgcgtg acgcgctcgc cggtgccctc
     7981 ggccggtgta gccggcgttg cctgcgttgc ggcggcggcc gattgcttcc agccgatctg
     8041 cttgagcttg cccggccgct ccttggcgcc ggccacgaac gccgcgccgg gcagcacgat
     8101 cgcggcggcg agcaacatca ccagccagca ccaccacggg gtgcggcgtt gacatccgtg
     8161 tcccaggcgc atgattcgct ccaatcggtt cgaggtgaca tcgaccggcc gcacgccggg
     8221 aaacgccggc acggccaaca gttcgtgttt gagctgcagg atgtcgagca ggctgcgggc
     8281 atagcgggcc ggctcgcagc ctagctcggc cagcacctgc tcgtcgcagc agcgctcggc
     8341 ctcgcgcgag aggaggcgat tggcgagcca gaccagcggg tggaaccacc acagcgactg
     8401 cgccagcacc tgtgccgccg ccagcc
//
LOCUS       DASRJB010000441         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_972407, whole genome shotgun sequence.
ACCESSION   DASRJB010000441 DASRJB010000000
VERSION     DASRJB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972407"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..212)
                     /locus_tag="VFB80_11630"
     CDS             complement(78..212)
                     /locus_tag="VFB80_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34466.1"
                     /translation="MPKQYESMREKFMQQGLSKKDAQAKAAKIYNAAHPRKPVGRGKK
                     "
     gene            complement(247..546)
                     /locus_tag="VFB80_11635"
     CDS             complement(247..546)
                     /locus_tag="VFB80_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZN34467.1"
                     /translation="MSKSETLKPLADRVVIRPNEPEKMTPGGIVIPDAAKEKTNRGEV
                     LAVGPGRLLENGDYDTPRIAVGDVVIYDRWAGNEIDLNGDKVRVMRAGDVLAVVK"
     gene            complement(580..1038)
                     /locus_tag="VFB80_11640"
     CDS             complement(580..1038)
                     /locus_tag="VFB80_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34468.1"
                     /translation="MSRLEHLRPGNFVVVVGCRNLAAYAAYNPYREEYEPLQFPGVPL
                     RIVELGLPFACVTDGQKTFAIDLRDFDVQKVGGKYAAAMAGYCTDPRQAEQKRTALLG
                     QAAPPAAADPKVCPRCGQRMVERRTAGIQGWFLTCSTCGHNGGPVLVATP"
     gene            complement(1035..1289)
                     /locus_tag="VFB80_11645"
     CDS             complement(1035..1289)
                     /locus_tag="VFB80_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34469.1"
                     /translation="MPNSSLHVIRLVGGPQDGLPLVVASNKVLIIVAARPSDEDDWND
                     LVAPAYRRRGETQRFDFVGWTNREQLAADDEIDTADWWKR"
     gene            complement(1293..1592)
                     /locus_tag="VFB80_11650"
     CDS             complement(1293..1592)
                     /locus_tag="VFB80_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34470.1"
                     /translation="MTPQPPAADLEPRTIPPTIRRAAFHRGLPLPPEPARPLTPPPSE
                     HHVAPEPILEEPPPAEFESERGRPRPKLHAQPERQSRRKLKRLLPAKLRAYRQRK"
     gene            complement(1589..1963)
                     /locus_tag="VFB80_11655"
     CDS             complement(1589..1963)
                     /locus_tag="VFB80_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34471.1"
                     /translation="MRWIRADYQLETSQRSARGMLILVWAKHQAAHRDVMGFLERFEN
                     DPDGIEKSIVELIDRYRPELKGCEVWGMHYDPMLQRWEFFVTHQSLSADTWPAGLSGA
                     GRTGDGLATISLVPPPREELPL"
     gene            complement(1966..2214)
                     /locus_tag="VFB80_11660"
     CDS             complement(1966..2214)
                     /locus_tag="VFB80_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34472.1"
                     /translation="MPLLDERRFPLKDGSSLNIDRCARTLKEWHKKGVLIKRNGRKVR
                     IRLEVLREGNRLVTSREAVKRFLDATNPELSKLNGHKE"
     gene            2366..>3023
                     /locus_tag="VFB80_11665"
     CDS             2366..>3023
                     /locus_tag="VFB80_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34473.1"
                     /translation="MDPAQPNTPAETTPATPPAAPPNAGAPPAAPATPPNPRLAKLAA
                     LGFENVKDEGEALDRLYDAHVQMKDQFGQQLQQTLEQVRAQVQPPAAPEAGQRPKFFD
                     PPKVDLAVVAQYREADGNWKENTPAEVKNAALAYQGYVEAFGRKLLTDPEATLAPLIE
                     RIAARVAEERYGQATAQQQEAQFFERAYAENEWLFEKSPHTGRVDRQRLSAEGQRFND
                     L"
BASE COUNT          505 a          990 c         1029 g          499 t
ORIGIN      
        1 gctccgtgag gccggtgcgg cggtagaggg tcgggccgaa gagggtcacc cacaatcgtc
       61 ggcgtcctgg cgcatgctta cttcttgccg cggccgaccg gcttgcgggg gtgcgcggcg
      121 ttgtagatct tcgcggcttt ggcctgggcg tctttcttgc tcaggccctg ctgcatgaat
      181 ttttcccgca tggattcgta ctgcttgggc atttgagggt ctccctgaaa aaggcgatgc
      241 agcgattcac ttcacgaccg cgagcacgtc tccggcccgc atcacccgga ccttgtcgcc
      301 gttgagatcg atctcattcc cggcccagcg gtcatagatg accacgtcgc ccacggcgat
      361 gcgcggggta tcgtaatcgc cgttttcgag caagcggccg gggccgaccg ccaggacttc
      421 gccgcggttg gtcttctcct tggcggcgtc gggaatcaca attccgcccg gcgtcatctt
      481 ctcgggctcg ttgggccgga tcacaacgcg atcggccagc ggcttcaggg tctcggactt
      541 ggacataacg atctcctcgc gtgaaaagga aatgggaaac tacggggtgg cgaccaggac
      601 ggggccgccg ttgtggccgc aggtgctgca ggtcaggaac cagccctgaa tgccggcggt
      661 gcggcgctcg accatccgct ggccgcagcg ggggcagacc ttgggatcgg cggcagccgg
      721 cggcgcggcc tggcctaaga gcgcggtacg cttctgctcg gcctggcggg ggtcggtgca
      781 gtaaccggcc atcgccgcgg cgtacttgcc gccgaccttc tggacgtcga agtcccgcag
      841 gtcgatcgca aacgtcttct ggccgtcggt gacgcaggcg aagggcagac ccagctcgac
      901 gatccgcagc ggaacgccgg ggaactgcag cggctcgtac tcttcgcggt aggggttgta
      961 ggcggcgtag gccgccaggt tgcggcagcc gacgacgacc acgaagttgc cgggacggag
     1021 atgttcgagg cggctcatcg cttccaccag tcggcagtgt cgatctcatc gtcggccgcc
     1081 agctgctcgc gattggtcca gccgacgaag tcaaagcgtt gggtctcgcc gcggcgacgg
     1141 taggccggcg cgaccagatc gttccagtcg tcctcgtcgc tcggccgagc ggccacaatg
     1201 atcagcacct tgttgctggc cacaaccagc ggcaggccat cctgggggcc gccgacgagc
     1261 ctgatgacgt ggagcgagga gttcggcatc gatcacttcc gctgacggta ggcccgcagc
     1321 tttgccggca gcaggcgttt gagctttcgc cgcgactgcc gctcgggctg ggcgtggagc
     1381 ttgggccggg gccgcccccg ttcggactcg aactcggccg gcggcggctc ctcgaggatg
     1441 ggctccggcg cgacgtgatg ctcactgggt ggaggcgtca gcggccgcgc cggttcgggc
     1501 ggcagcggca ggccgcggtg aaaggcggcc cggcgaatcg tcggcgggat cgtgcgcggc
     1561 tcgaggtcag cggcgggggg ctgcggcgtc atagcgggag ctcttctctt ggcggcggga
     1621 ccagggaaat cgttgccaag ccgtcgcccg tgcgcccggc ccccgacagg ccagcgggcc
     1681 acgtgtcagc cgacagcgac tggtgcgtga caaagaactc ccatcgctgc agcatcgggt
     1741 cgtagtgcat tccccagact tcgcagccct tcaattcggg acggtagcga tcgatcagct
     1801 caacgatcga cttctcgatg ccgtcggggt cgttctcaaa tcgctcaagg aaacccatga
     1861 cgtcgcggtg agccgcctga tgcttcgccc acaccaagat gagcatgccc cgtgcagagc
     1921 gctgactggt ctcaagttga taatcggcgc ggatccaacg cataactact ccttgtggcc
     1981 gttgagcttg gagagctcgg ggttggtcgc gtcgaggaac cgcttgacgg cctcgcgcga
     2041 ggttaccagc cggttgccct cgcggagcac ttccagccgg atgcggacct tcctgccgtt
     2101 ccgcttgatc aagacgccct tcttgtgcca ctccttcagg gtccgggcgc agcggtcgat
     2161 gttcaggctg ctgccgtcct tcagcgggaa gcggcgctcg tcgaggaggg gcatgccgcc
     2221 aatgtagaaa ccacccctgg attattcaag agaaggatgg aacaggccgg aaagatttcc
     2281 cgccgcgcat tgatcgcaac cggctgtgtg gcagagagtt tgcgcgttgg aaccacgcga
     2341 accactgccg cacaaggact tgcgtatgga ccccgcccag ccgaacacgc cggccgaaac
     2401 cactcccgcc acccctcccg ctgcgccgcc aaacgctggc gcgccgcccg ccgcgccggc
     2461 tactccgccg aatccgcgac tggcgaaact ggccgcgctg ggcttcgaaa acgtcaagga
     2521 cgagggcgag gccctggacc gcctgtacga cgcccacgtc cagatgaagg accagttcgg
     2581 gcagcagctg cagcagaccc tggaacaggt ccgcgcccag gtccagccgc ccgccgcgcc
     2641 ggaagccggc cagcgcccca agtttttcga tccgcccaag gtggacctgg ccgtggtcgc
     2701 gcagtaccgc gaggccgacg ggaactggaa agaaaacacg ccggccgagg tcaagaacgc
     2761 ggccctggcc tatcagggct acgtcgaggc ttttggccgc aaactgctga ccgaccccga
     2821 agccacgctg gccccgctga tcgagcggat cgccgcccgg gtcgccgaag agcgttacgg
     2881 ccaggcgacg gcccagcagc aggaagccca gttcttcgag cgggcctatg ccgagaacga
     2941 gtggctgttc gaaaagagcc cgcacaccgg ccgcgtggac cggcagcggc tcagcgccga
     3001 agggcagcgg ttcaacgacc tga
//
LOCUS       DASRJB010000442         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3503990, whole genome shotgun sequence.
ACCESSION   DASRJB010000442 DASRJB010000000
VERSION     DASRJB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503990"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFB80_11670"
     CDS             complement(<1..960)
                     /locus_tag="VFB80_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34474.1"
                     /translation="MNRRHFLSCLFVLVVAFGFQPAQAAEPRLEFTRMVAHWAEYGHP
                     DYLPFIYEAEPELVQLGFYGAHFYSLAHTPAFGGYPAHFPVQGLNECGAWFEEKNRRL
                     HEKQIKVVGHFNVEFLVGDPDGPKGPTGFFKFYRELWDEKELGKRPVDDPLQLLERGP
                     DGQPITQDGYGIGGMKEYWGCLRNPAWQAVLKAWVKRGIERGVDGYIANYFYKHNCHC
                     EHCQREFRAYMKERHSAEELKAAYSISDLATHKFEEMAYWHDPAQTTPLKLEMLRWAQ
                     VSNKQVFDKVFIDYGRSLKKDLIVAQWNHLGDFGQISGDERCLL"
     gene            complement(965..>2030)
                     /locus_tag="VFB80_11675"
     CDS             complement(965..>2030)
                     /locus_tag="VFB80_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN34475.1"
                     /translation="SLAVVGYTAIGGFLAAVWTDLLQSVMMFAGVTLLACLVLTKAGG
                     LEAGTKRAIEHAGPQFATGPGLKVQKDAEGNIQEIAFLPLTMAFSMFVFWPFAGTANP
                     ASVVRVMACKNTAVLRRSIFLLAVYNMGIYLPLIAICICGRGLMPDLGKESDAIVPRL
                     ALSLTEGIPLGSWLSGLILAAPFGAIMATVSCFLLVIASGLVRDVYQRFLNPAATEYE
                     LRRLTYVTMALIGLIAMLLNINPVEKLQMLVTFSSGCAASAFLVPTAMMCYWRRATAG
                     GVISAMLAGSATAFSLNMAGLVISIVNKSGFVPYNLLGFEPIVWGLAVSLLAGIAASL
                     LTAPPDEKLVSKLFDAPEPA"
BASE COUNT          393 a          670 c          620 g          347 t
ORIGIN      
        1 cagcaggcag cgctcgtcgc cgctgatctg gccaaagtcg cccaggtgat tccattgcgc
       61 gacgatcagg tccttcttca gcgagcggcc atagtcgatg aagaccttgt cgaagacttg
      121 cttgttggag acctgcgccc agcggagcat ctcgagcttc agcggcgttg tctgagctgg
      181 atcgtgccaa taggccattt cctcaaactt atgagttgca agatcgctga tgctatacgc
      241 ggccttcaac tcttcggccg agtgccgctc tttcatgtaa gcgcggaact cccgctgaca
      301 atgctcgcag tggcagttgt gcttgtagaa atagttggcg atgtagccat cgacgccgcg
      361 ctcgatgccg cgcttgaccc aggccttcaa gaccgcctgc caggccggat tccgcaggca
      421 gccccagtac tccttcatcc cgccgatgcc atagccgtcc tgcgtgatcg gctggccgtc
      481 cgggccgcgc tctaacaatt gcaggggatc gtcgaccggc cttttcccca attccttttc
      541 atcccatagc tcgcgataga acttgaaaaa gccggtgggg cctttggggc catcgggatc
      601 gccgaccaga aactcgacgt tgaagtggcc gacgaccttg atctgcttct cgtgcagccg
      661 gcggttcttt tcctcgaacc atgcgccgca ctcgttcagg ccctgcacgg gaaaatgcgc
      721 cgggtaaccg ccgaatgccg gcgtatgcgc caggctgtag aagtgagcgc catagaagcc
      781 gagttgcacc agctccggct cggcttcgta gatgaacggt agatagtcgg gatgaccata
      841 ctcggcccag tgggccacca tccgcgtgaa ctccagccgc ggctccgcgg cctgggcggg
      901 ctgaaacccg aaggccacga cgagcacgaa gaggcacgaa aggaagtggc ggcggttcat
      961 gggcttaggc cggctccggg gcgtcgaaca gcttgctcac cagcttctca tcgggcggcg
     1021 cggtgagcag gctggccgcg atgccggcga gcagcgatac ggccaggccc cagacgatcg
     1081 gttcaaagcc cagcaggttg tagggcacaa accccgactt gttgacgatc gaaatgacga
     1141 gtcccgccat gttcagcgag aacgccgtgg ccgacccggc gagcatggcg ctgatcacgc
     1201 cgccggccgt cgcgcgccgc cagtagcaca tcatcgccgt cggcacgagg aacgccgaag
     1261 ccgcgcagcc ggagctgaac gtcaccagca tctgcagctt ctcgaccggg ttgatgttca
     1321 gcagcatggc gatcaggccg atcagcgcca tggtgacgta ggtcagccgc cgcagctcgt
     1381 actcggtcgc cgcggggttg aggaaccgct gatagacgtc ccgcaccagt ccggaggcga
     1441 tcaccaggag aaagcagctc accgtggcca tgatcgcgcc gaacggcgcc gccaggatca
     1501 gccccgacag ccaggagccc agcgggatgc cctccgtcag gctcagcgcc aaccgcggca
     1561 cgatcgcgtc gctctccttg ccgaggtcgg gcatcagccc gcggccgcaa atgcagatcg
     1621 caatcagcgg caggtaaatc cccatgttgt agaccgccag caggaagatc gaacgccgca
     1681 gcacggccgt gtttttgcag gccatgacgc ggacgacgct ggccggattg gccgtacccg
     1741 caaacggcca gaacacaaac atcgagaacg ccatcgtcag cggcaggaag gcgatttcct
     1801 ggatgtttcc ctccgcgtcc ttctgcacct tgagccccgg cccggttgcg aactgcgggc
     1861 cggcgtgctc gatcgcccgc ttcgtaccgg cttccaatcc gccggctttc gtgagcacca
     1921 ggcacgccag caacgtcacg cccgcgaaca tcatcacgct ctgcaagagg tcggtccaca
     1981 ctgccgccag gaagccgccg atggccgtgt agccgaccac tgccagcgaa
//
LOCUS       DASRJB010000443         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2089593, whole genome shotgun sequence.
ACCESSION   DASRJB010000443 DASRJB010000000
VERSION     DASRJB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089593"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3674)
                     /locus_tag="VFB80_11680"
     CDS             complement(<1..3674)
                     /locus_tag="VFB80_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34476.1"
                     /translation="MARQRIRPEEDDDRESRESNKPRKGGSWTFWLGSRLLVVLLLLL
                     VLAFFAPMLIGSTGAWKSIVASTAPKLADKLDAASVTLSWFAPIEMTGVAIRDAAGEP
                     LAEVARVRSRKTLLEIALNYHDLGTFDIEEPKARLSLRAGGSNAEDFLALLPKSEGES
                     PATQIGLAISRGQIQLDDTIAGRQWLLSDVTFDLLWTAAADQPKTGKLSAAVSPAAAA
                     AANPAQIAAEFSWLPPTDGKGSLGAGQAQFMLSGVPAELTEGALRRFVGDIRPAGPLT
                     LEGTYVWTQNGQSHQANIKKLSTPGVALSAPQWLGADQPRIAIRSGQGDVQLAQGKIT
                     ARGVQLDSNWLQLAGTGTAVLSSLRESDLQFRGECDLAEVARQLPSLLRVKQGTQLSS
                     GAIRFVLNQEEEGKAMGRTWNAALETNILRATIDGRPVAFLEPISLNLRVHETQASWG
                     EAEIAGRAAFFNLAGRGTLAGGSLKADADLDKLIAELGQVLDVSGLKLSGKLNTDIQW
                     KHDESIGWLAAADASVQNFAMQSAGLAPWQEPNLKLAAELRGAIGPRGLERIDAGRLS
                     VVAGADQLAAELAEPVQSPSASTVWPLRFSLKGDLATWTPRIQPLVALGDWRLSGAID
                     AVGAGKFSPQSVELTPTGISIEPFALQGGGLSIREPRIKIDTAGAWDQQRATLTLGTT
                     TFASSALAFRADGLRVVAGQEPSLVGVIDVRGDLAKLHGWLDAGNQARTSLLSGALTG
                     RVEVGYRGQTLAAAWQTDVENFAMLVPQQPAGGGGSATLAAAASPQWQPLLPPEHVNL
                     TGQGSYDPSAGTLKIERTALTASTLSLAAAGTVKSVTAAPQVDLSGEIAYDLERLTQQ
                     IQAFYAKRNASGVLVLPYGLNTLQLQGNERRQFVLKGPLFAATDAQSRDQWSRSGMPS
                     TATIGRGFGIAEALTGEASLGWQGAQYVGLVSGAADCRARLAGGIINIGPLDIPVSEG
                     RLTTAPRVLLNSPVPAVVVERGPLIQNVRISPEMCSLWLKFVAPLVAEATRAEGKFSL
                     SLEGAAVPIADPVTSDVTGTLAIHQAQIGPGPLAQQYLGMAQQLRSFFDAKAGEAAAV
                     DPNRGWLILPQQDVPFEVRQGVVHHHGLTMTVKDVVITTEGSVGIETQQINLLASIPV
                     QESWLKNDAKFAFLKGQTIKVPIRGTLSQPQLDGKVLENLGKQLVGNVVQGQINKQVE
                     RGQELIQREFDKGLNRLFGPL"
     gene            3879..4607
                     /locus_tag="VFB80_11685"
     CDS             3879..4607
                     /locus_tag="VFB80_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34477.1"
                     /translation="MSLPDPLHVCVALGPLAMYLIVLGLINLSRRPLLTTGGRDVAAL
                     ALALGGLVAAGPMELFMVEEAAVQFGGWVWAILLTGYCLGVVLLILTIRPRIIIYNTS
                     TDEIRPILEAVVARLDSEARWAGESLVMPQLGVQLHLESWPMMHNVQLVSAGSDQNLL
                     GWRRLEIELAGVLRKTRHAGNLFGVVAASCGLFLAALLTWNLASNPAAVAQAWNDMLR
                     HPPPPAPLEPAPPDPAPGASDANP"
     gene            4594..>5100
                     /locus_tag="VFB80_11690"
     CDS             4594..>5100
                     /locus_tag="VFB80_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34478.1"
                     /translation="MPILDRFRLTGKRLFITGGSRGLGREMALACADAGADCVLVGRE
                     AESLQKTAGDVRGLGRQAFTVQADVSKPAECEAACRRALDEFGPIDILINNAGGRRQN
                     IATDEMPLEVWRELMDLNLTSTFICTKQIGGAMLARGRGGRIINIASINALVAGRKIA
                     GRHYETSKA"
BASE COUNT          791 a         1725 c         1703 g          881 t
ORIGIN      
        1 agcgggccaa acagccggtt gaggcccttg tcgaattcgc gctggatcag ctcctggccg
       61 cgctcgacct gcttgttaat ctgcccctgg actacgttgc cgacgagctg cttgcccaaa
      121 ttctccagca ctttgccgtc gagctgcggc tgcgagagcg tgccgcggat cggcaccttg
      181 atcgtctggc ccttgagaaa cgcgaacttc gcgtcgttct tcagccagct ctcttgcacc
      241 ggaatgctgg ccagcaggtt gatctgctgg gtctcgatgc cgacgctgcc ttcggtggtg
      301 atgaccacgt ctttgacggt catcgtcagt ccgtggtgat gcacgacgcc ctggcggact
      361 tcgaacggca cgtcctgctg cggcagtatc agccagccgc gattgggatc gacggcggcc
      421 gcctcgccgg ctttggcgtc gaagaacgag cggagctgct gggccatgcc caggtattgc
      481 tgggcgagcg ggccgggacc gatctgggcc tggtgaatcg ccagcgtgcc ggtcacgtcg
      541 ctcgtcacgg gatcggcgat cggcaccgcc gcgccctcca acgagagcga gaatttgcct
      601 tcggcccgcg tggcctcggc gaccagcggc gcgacgaact tgagccacag gctgcacatc
      661 tcgggcgaga tgcggacgtt ttgaatcagc gggccgcgct cgacgacaac cgccggcacg
      721 gggctgttca gcagcacgcg cggcgcggtc gtcagccggc cctcgctgac cggaatatcc
      781 agtggtccga tgttgataat tccgccggcg agcctggccc ggcaatccgc ggctcccgag
      841 accaggccga cgtactgcgc gccttgccag ccgagactgg cttcgccggt aagcgcctcg
      901 gcgattccaa agccgcgacc aatggtcgcg gtgctcggca ttccggaccg cgaccactgg
      961 tcgcggcttt gagcatcggt cgcggcaaac agcggccctt tcagaacgaa ctgccggcgc
     1021 tcattgccct gaagctgcag cgtattcaga ccgtacggca gcaccagcac gccgctggcg
     1081 ttgcgcttgg cgtagaaggc ctgaatctgc tgcgtcaggc gttccaggtc gtaggcaatc
     1141 tcgcccgaca gatcgacctg gggcgcggcg gtgacgctct tgaccgtgcc ggcggcggcc
     1201 aggctgagcg tgctggccgt cagcgccgtg cgctcgatct tgagcgtgcc cgcgctgggg
     1261 tcgtacgagc cttgaccggt gagattgacg tgttccggcg gtaagagcgg ctgccattgc
     1321 ggcgacgccg ccgcggccag ggtcgccgag ccaccgccgc cggccggctg ctgcggcacg
     1381 agcatcgcga aattctccac gtccgtctgc caggcggctg ccagcgtttg cccgcgatag
     1441 ccgacttcga cgcgcccggt cagtgcacca gagagcagcg acgtccgcgc ctgattgccc
     1501 gcatcgagcc agccgtgcag cttcgccagg tcgccgcgga cgtcgatcac gccgactaac
     1561 gaaggctcct ggccggcaac cacgcgcagg ccgtcggccc ggaaggcgag cgcggagctg
     1621 gcgaacgtcg tcgtgccgag cgtcagggta gcccgctgct gatcccacgc gccggccgtg
     1681 tcgatcttga ttcgcggctc gcggatggac agtccgccgc cttgcagcgc gaagggctcg
     1741 atcgagatgc ccgtcggcgt caactcgacc gactgcggag agaatttgcc agcgccgacg
     1801 gcgtcgatcg cgccggacag ccgccaatcg ccgagcgcaa cgagcggctg aatgcgcggc
     1861 gtccaagttg ccaggtcccc cttcaggctg aaccgcagcg gccacacggt ggacgccgac
     1921 ggcgactgga cgggctcggc caactcggcc gccagttgat cggcgcccgc gacgaccgac
     1981 agccggcccg cgtcgatgcg ttccagtccg cgaggcccga tcgccccgcg caattccgcc
     2041 gccagcttga gattgggctc ctgccacggc gcgaggcccg ccgattgcat cgcgaagttc
     2101 tggacgctcg cgtcggcggc agccagccat ccgatgctct cgtcgtgctt ccactgaatg
     2161 tccgtgttga gttttcctga aagcttgagg cccgagacgt ccagcacctg ccctaactcc
     2221 gcgatgagct tgtcgagatc ggcgtcggcc ttgagcgatc ctccggccag cgtgccgcgc
     2281 ccggccaggt tgaagaacgc ggctcgcccc gcgatttcgg cctcacccca gctcgcctgt
     2341 gtttcgtgca cgcgcaagtt gagcgagatc ggttccagga acgcgacggg gcggccgtcg
     2401 atcgtcgcgc ggagaatgtt cgtctccagc gcggcgttcc aagtgcgccc catcgccttc
     2461 ccttcctctt cctggttcag cacgaagcgg atggctccgg acgacagctg cgtcccctgt
     2521 ttgacgcgga gcagcgaggg cagttgccgc gcgacctccg ccagatcgca ctcgccgcga
     2581 aattgcagat cgctctcgcg aagactgctg agaacggcag tccctgttcc ggcgagctgc
     2641 agccaattgg aatcgagctg cacgccgcgg gcggtgattt ttccctgagc cagttgaaca
     2701 tcgccctgac cggacctgat cgcaattcgc ggctggtcgg cgcctagcca ctgcggcgcg
     2761 gacagcgcca ctcccggagt ggagagcttc ttgatgttgg cctggtgcga ctggccgttt
     2821 tgcgtccaga catacgtgcc ttccagcgtg agcgggccgg cggggcgtat gtcgccgaca
     2881 aagcgccgca gtgcgccttc ggtcagttcg gccggaacgc cactcagcat aaactgcgcc
     2941 tggcccgcgc ccagcgagcc ttttccgtcc gttggcggaa gccaggagaa ttcggccgcg
     3001 atctgagccg gattggcggc cgccgcagcg gctggactga ccgccgcgga cagcttgccc
     3061 gtcttcggct gatcggcagc cgcggtccat agcaggtcga aggtcacgtc gctgagcagc
     3121 cactgcctcc cggcgatcgt atcgtcgagc tggatttgcc cgcgcgagat cgccaggccg
     3181 atttgcgttg caggcgactc gccttccgac ttcggcaata gcgccagaaa atcctcggcg
     3241 ttgctgccgc cggcgcgcag cgagagcctg gccttcggct cctcgatatc gaacgttccc
     3301 aggtcgtgat agttgagggc aatctccagc agggtcttgc ggctgcgaac gcgggccact
     3361 tccgccagcg gctcgccggc ggcatcgcgg atggcgacgc cggtcatctc gatcggcgca
     3421 aaccaggaga gcgtgaccga ggctgcatcg agcttgtccg ccaacttggg cgcggtgctc
     3481 gcgacgatcg atttccatgc gccggtgctc ccgatgagca tcggggcgaa gaatgccagc
     3541 actaagagca gcagcagcac gaccagcagg cgcgagccga gccagaaggt ccacgagccg
     3601 cccttgcgcg gcttgtttga ctcgcgcgat tcgcggtcgt cgtcctcttc gggccgaatt
     3661 cgttgcctgg ccatctcgat cgcctcctcc gtgaggcctc cgactgacag aggcggcctg
     3721 gattgtaagc cgaggcagtg ctggcaacct agagcgactt ggctctggcg tcggcaggac
     3781 tggatcgtcg caactgcttt cctgccagca ccttgtgtcg ccggatggcc gctcctagaa
     3841 tcgacgaaac gcgcgcccgc tccccgctgg acccatccat gtcgctgccc gatccgctgc
     3901 atgtttgcgt ggcgctgggt ccgctggcga tgtatctgat cgtgctggga ctgatcaatc
     3961 tttcgcgccg gccgctgctc acgaccggcg gccgcgatgt cgcggcgctg gctctggcgc
     4021 tgggcggttt ggttgccgct gggccgatgg agctgtttat ggtcgaagaa gccgccgtgc
     4081 agttcggcgg ctgggtgtgg gcgattctcc tcacgggcta ttgcctgggc gtcgtcctgc
     4141 tcatcctgac gatacggccg cggatcatca tctacaacac gagcaccgat gagattcggc
     4201 cgatcctgga ggcggtcgtc gcccggctcg actccgaggc ccgctgggcc ggcgaatcgc
     4261 tggtcatgcc gcagctcggc gtgcagctgc acttggagtc gtggcccatg atgcacaacg
     4321 tgcagctcgt ttcggctggg tcggaccaga acttgctcgg ctggcggcgg ctcgaaatcg
     4381 agctggcggg tgtactgcgc aagacgcggc acgccggcaa cctgttcggc gttgtggccg
     4441 ccagctgcgg cctgttcctg gccgcgctgc tgacctggaa cctggccagc aatccggccg
     4501 cggtcgcgca agcctggaac gacatgctcc gccacccgcc gcccccagcg ccgctcgagc
     4561 cagcgccgcc ggatcctgca ccaggagcct ccgatgccaa tccttgatcg tttccgcctg
     4621 acgggcaaac ggctgttcat caccggcggc agccgcgggc tgggacgcga gatggccctg
     4681 gcctgcgccg acgcgggcgc cgactgcgtg ctcgtcggcc gcgaggcgga gagcttgcag
     4741 aaaacggccg gcgacgtccg cggtctgggc cggcaggcat tcacggtgca ggccgacgtc
     4801 agcaagccgg cggaatgcga agccgcctgc cggcgggccc tcgacgaatt cgggccgatc
     4861 gacatcctga tcaacaacgc gggcggccgg cggcagaaca tcgctacgga cgagatgccg
     4921 ctggaagtct ggcgcgagct gatggacctg aatctgacca gcacgttcat ctgcaccaag
     4981 cagatcggcg gcgcgatgct ggctcgcggc cgcggcgggc ggatcatcaa cattgcctcg
     5041 atcaacgccc tggttgctgg ccgcaagatc gcgggccggc actacgaaac gagcaaagcg
//
LOCUS       DASRJB010000444         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3223867, whole genome shotgun sequence.
ACCESSION   DASRJB010000444 DASRJB010000000
VERSION     DASRJB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223867"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFB80_11695"
     CDS             complement(<1..640)
                     /locus_tag="VFB80_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34479.1"
                     /translation="MGIRLFLGVGMALALASATAAEEAAKIDGYWTLVHDEAVLADLK
                     LTPEQRAAWRKMLDPLDVSFFPFRGRSASEATKALLERTEQAQAHAAKILTAAQLQRL
                     AKLQVRLEGTEALLRDDFAAQLKLTDEQRADVRKRIEDTRAALAKVNSQVVAGSLEDA
                     AARKEMERLNKAQWDGVLKTVTKEQQGVWSSLLAGDFDPAKLGQTKFKSPDLI"
     gene            752..934
                     /gene="rpmF"
                     /locus_tag="VFB80_11700"
     CDS             752..934
                     /gene="rpmF"
                     /locus_tag="VFB80_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZN34480.1"
                     /translation="MAVPKRRQSNSRTRRRRAHHAKKPKQLSYCPKCSAAVPQHVVCP
                     NCGYYMGRVIVESEEE"
     gene            1011..1922
                     /gene="fabD"
                     /locus_tag="VFB80_11705"
     CDS             1011..1922
                     /gene="fabD"
                     /locus_tag="VFB80_11705"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZN34481.1"
                     /translation="MTKPAFLFPGQGAQTVGMGRQLVESTPRAREVFERAKAVLGYDL
                     LAVCLEGPAEKLDATDFSQPALFVTSLAALEWLRANKPEVHDGCRGVAGLSLGEYTAL
                     VFAGVMSFEDGLRVVQERGRAMQAASDAVPSGMVSILGLDAEKTEQVCGEARSAGEVL
                     QIANYLCPGNIVVSGHKTACARVADLAVKAGAMKAIPLAVAGAFHTPLMQPAVDRLTK
                     ALAGVKLNPPRIPVISNVDAAAHSDPEEIRELLVKQVVSPVRWEDSLRNLLAEGYAPF
                     WEVGPGRVLRGLLKRIDRKAESDGVEC"
     gene            2020..2790
                     /gene="fabG"
                     /locus_tag="VFB80_11710"
     CDS             2020..2790
                     /gene="fabG"
                     /locus_tag="VFB80_11710"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZN34482.1"
                     /translation="MADNPYSSLKVDLTGQTAVVTGASQGLGKAIAMALAAAGAKVAC
                     VARSADKLKDTVAAITAAGGQAEAFPCDVKESAAVDALIDGIAEKWGKIDILINNAGV
                     TRDTLLPRMSDQEWDDVINTNLRGSFLFARACSRHMMRARYGRVINISSVSGLMGNAG
                     QTNYSASKAGLIGFTRSMSRELAGRKVTINAVCPGFIESDMTKVLGEMIVDEVKKRIP
                     AKRLGLPEDVATCVLFLASPAACYVTGQVLTVDGGMTG"
     gene            3003..3242
                     /locus_tag="VFB80_11715"
     CDS             3003..3242
                     /locus_tag="VFB80_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN34483.1"
                     /translation="MASVAERVIEIVAEQLGVDKEKITPETSFVNDLGADSLDTVELV
                     MELEEEFDINIPDDAAEKIQTVGQAIKFIEEAQNG"
     gene            3320..4588
                     /gene="fabF"
                     /locus_tag="VFB80_11720"
     CDS             3320..4588
                     /gene="fabF"
                     /locus_tag="VFB80_11720"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZN34484.1"
                     /translation="MKRRVVVTGLGCVTSLSCQVEDLWQRLLRGESGVHPLRILDTER
                     FKVKFGGDVYDWAPEKWPAAFVEKPDSFTEKKEIKRLDRFSQFAMVAGWQAVQDAGLD
                     FTREDVWRCGVILGSGIGGLNEIEEQCTKLFEKGPDRVSPFVVPKMMLNAAGGNLSIR
                     YGLRGLNYSVATACASANNAVGDALQAIQHSALDVAITGGTEAAITQMGLAGFANMKA
                     LSMRNDDPPRASRPFDSERDGFVLSEGAGLLILEELEHAQQRGARIYCEVVGFGASAD
                     AGHITQPDEQGAGAGRAMSDALADAQLNATAIGYINAHGTSTPLGDAAETQAIKRVFG
                     DHARAVSISSTKSQLGHSLGASGGIELVVTVKALASSAIPPTINLTSPDPACDLDYTP
                     NTARERRFTHAMSNSFGFGGHNASIVIAKL"
     gene            4814..6214
                     /locus_tag="VFB80_11725"
     CDS             4814..6214
                     /locus_tag="VFB80_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34485.1"
                     /translation="MPAKSWFVAILLAGAAAPLGAADEFPYTAYVALEGAEVVAGPGH
                     RYYATEHLPRGTPVEVYREEASGWLAIRPPDSSFSWVPAKSVERAPDDRSAGRVTGPT
                     PAWVGTSVEHVGEHRQQVTLKEGELLQIIGEKEVAADGAEKEVWLKIAPPAGEFRWIH
                     LRDVSRQAPPPALPEPEPEVDEQPQRPEPAEPRRLDVAGPPIALADIERPKSRQLALV
                     ERAQFQNSGGLSNPLSPDGFVPRKRTGSEHLQPPAVPPAGLTRTSTPTIRSRLASESP
                     REPAAPAPVSASGGAGDLAAQLERLELDLSLMVAKDKTLWDLASIKTRVRDLVDHGPD
                     PAGRGRARLLLDKVEQFEQAFAVQNYGPIRTGATAAAAKSKPASPGMEDPRYDAQGWL
                     KPVISRQGDKPVAPYAVVDQDGQPLCFITPQPGINLRRYENKHVGVYGRRGFLEELKK
                     PHVTAERIIDVDRQWR"
     gene            complement(6439..7044)
                     /locus_tag="VFB80_11730"
     CDS             complement(6439..7044)
                     /locus_tag="VFB80_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34486.1"
                     /translation="MRARTTSWWLGVLAASVLVAAGAVRIWQARQSAALQVSQQRYQA
                     QIAALPERQAARLVSLLAEDDQRLEIVARAWADPRPQVARSGRLGVQSAANRWSQLTP
                     EEAAPRVAALASLLARHAPQTSALDRDAARALAQRLLDWPVDSRQIDAARLIGDCDSV
                     LRLDSPAPEELRLAAAPAPPAQEIAAPLPPAAPPPAPRRLN"
BASE COUNT         1280 a         2354 c         2474 g         1190 t
ORIGIN      
        1 caatgagatc cggcgatttg aatttcgtct ggccgagctt cgccggatcg aagtcgccgg
       61 ccagcaggct gctccagacg ccctgctgct ctttcgtaac cgttttcagc acgccgtccc
      121 actgagcctt gttcagccgc tccatttcct tgcgggcggc agcgtcctcc aggctgccgg
      181 cgacgacctg gctgttgact ttggcgagcg cggcgcgcgt gtcctcgatc cgtttcctta
      241 cgtcggctcg ctgctcgtcg gtgagcttga gttgggcagc gaaatcgtcg cgcaagagcg
      301 cctcggtgcc ttccaggcgc acttgcagct tcgcgaggcg ctggagctgc gccgcggtga
      361 gaattttggc ggcgtgtgct tgggcctgct ccgttcgctc cagcagggct ttggtcgcct
      421 cagacgccga gcggccgcgg aagggaaaga agctcacgtc caagggatcg agcatctttc
      481 gccacgccgc acgctgctct ggcgtgagct tgaggtcggc cagcacggcc tcgtcgtgga
      541 cgagcgtcca atagccgtcg atcttggctg cctcttccgc ggcggtcgcc gaggccagcg
      601 ccaacgccat ccctacaccc aaaaacagtc ggattcccat cgccgatcct ccagttggcg
      661 tgctggctcg ccaacttgac agcttttccg atgaccttta gcatcacctg tttccactga
      721 atcgaccagc cccgccgggg cagagtgtcc catggccgtt cctaaacgcc ggcagtccaa
      781 ctcgcggacc cgccgccggc gcgcccacca cgccaagaag ccgaaacagc tctcgtactg
      841 ccccaagtgc agcgcggcag tgccgcagca cgtcgtttgc ccgaattgcg gctactacat
      901 gggccgcgtg atcgtggaat cggaagaaga ataggtttcg ggttccgagt tcaaagttca
      961 aagtcgagga atatcgagaa ctttgaacct tgagctttga actttgaact atgacgaagc
     1021 ccgccttcct gtttcccggc caaggcgccc agacggtggg catgggccgg cagctcgtgg
     1081 aaagcacgcc gcgggcccgt gaggtgttcg agcgggccaa ggcggtactg ggctacgatc
     1141 tgttggcagt ctgcctggaa ggaccagccg aaaagctcga tgccacggat ttcagccagc
     1201 cggcgctgtt tgtgaccagc ctggcggcgc tggaatggct gcgggcgaac aagcccgaag
     1261 tgcatgacgg ctgccgggga gtcgcgggcc tgagcctggg cgaatacacg gcgctggtct
     1321 tcgcgggcgt gatgagtttc gaggacggcc tgcgcgtcgt tcaggagcgc gggcgggcga
     1381 tgcaggcggc gtccgacgcg gtgccgagcg gcatggtgag catcctcggc ctcgacgccg
     1441 agaaaacgga acaggtctgc ggcgaagccc gctccgcggg cgaagtgctg cagattgcga
     1501 attacctttg ccctggcaac atcgttgttt cggggcacaa gaccgcgtgt gcgcgcgtgg
     1561 ccgacctggc ggtgaaagcc ggcgcgatga aggcgatccc cttggcggtg gccggcgcgt
     1621 ttcacacgcc gctgatgcag ccagcggtcg atcggctaac gaaggcgctg gccggcgtga
     1681 agctgaatcc gccgcggatt ccagtgattt ccaacgtcga tgccgccgcc cacagtgacc
     1741 cggaggagat tcgcgaattg ctggtgaagc aagtcgtctc gccggttcgc tgggaagatt
     1801 cgctccgcaa cctgctggcc gaaggctacg caccgttctg ggaagtcggc cccggccgcg
     1861 tgttgcgagg gctgctgaag cggatcgatc ggaaggcgga gagtgacgga gtggagtgct
     1921 gaggaaaatc caaagaaccg aagaaccgaa gaaccaaaaa tccaccgctt ccgatttggt
     1981 tcttaggttc tttggttctt ggaatttagg agccgagtga tggctgacaa tccctattca
     2041 agtctaaaag tcgatctcac cggccaaacg gccgtcgtca ccggcgcgtc gcaaggctta
     2101 ggcaaggcga tcgccatggc cctcgcggcc gcgggcgcga aggtcgcctg cgtcgcccgc
     2161 agcgctgaca aactcaagga caccgtcgcc gcgatcacag cagccggcgg ccaggcggag
     2221 gcgtttccct gcgacgtcaa ggagagcgct gcggtcgatg cgctgatcga cggcatcgcc
     2281 gagaaatggg gcaagatcga tatcctgatc aacaacgccg gcgtgacccg cgacacgctg
     2341 ctgccgcgga tgagcgacca ggagtgggac gacgtcatca acaccaacct ccgcggctcg
     2401 ttcctgttcg cccgggcctg ctcgcggcac atgatgcggg cccgctacgg ccgcgtgatc
     2461 aacatctcca gcgtcagcgg cctgatgggc aacgccgggc agacgaacta ttccgcgagc
     2521 aaggccggcc tcatcggctt cacgcggagc atgagccgcg aattggccgg ccgcaaagtg
     2581 acgatcaacg cagtgtgccc cggcttcatc gaaagcgata tgaccaaggt gctgggcgaa
     2641 atgatcgtgg acgaggtgaa gaagcggatt cccgccaagc ggctgggcct gcccgaggat
     2701 gtcgccactt gcgtactgtt cctggccagc cccgccgcct gctacgtgac cgggcaggtg
     2761 ctgaccgtcg atgggggaat gacagggtaa tttccaatga ccaggcacca atgaccaatg
     2821 ccaagccgac gaattggaca ttggggcttg gtcattggtg cccggctatc ttgaccctgt
     2881 ttgccgcttt cctctatcct gttgaattcg cttgacgacc ggcggcaaga ctcggtgaac
     2941 aaatacctgt acagggcaag gcccgcacag gccgcaaatc cctttcctgg aggacggtga
     3001 tcgtggcatc cgtcgcagaa cgcgtcattg agatcgtcgc cgagcagctc ggcgtcgata
     3061 aagagaaaat cacgcccgaa acttccttcg tgaacgacct cggcgccgat tcgctcgaca
     3121 ccgtcgagct cgtcatggag ctcgaggaag agttcgacat caacatcccc gacgacgcag
     3181 ccgagaagat tcagaccgtc ggccaggcca tcaagttcat cgaggaagcg caaaacggct
     3241 agggccgcgc agatttccgg agcggtcctg cgcggtaatt tccggaggtc ctgcgcttca
     3301 cctatcgcat gatgtctaga tgaaacggcg cgtcgtcgtt acggggctgg gctgcgtcac
     3361 atcgctcagt tgccaggtcg aggacttgtg gcagcggctg ctccgcggcg agagcggcgt
     3421 gcatcccctg cgcatcctcg acaccgagcg gttcaaggtc aaattcgggg gcgacgtcta
     3481 cgactgggcg ccggagaagt ggccggcggc gttcgtcgag aagcccgatt cgttcaccga
     3541 gaaaaaagag atcaagcggc tcgaccggtt ttcacagttc gcgatggtcg ccggctggca
     3601 ggcggtgcag gacgccggcc tcgatttcac gcgggaagac gtgtggcggt gcggcgtgat
     3661 cctgggcagc ggcatcggcg gcctgaacga gatcgaagag cagtgcacga agctgttcga
     3721 gaagggcccc gaccgcgtca gcccgttcgt cgtgccgaag atgatgctca acgcggccgg
     3781 cggcaacctg tcgatccgct acggcctgcg cggcctgaac tactcggtcg ccacggcctg
     3841 cgccagcgcc aacaacgcgg tcggcgatgc gctgcaggcg attcagcaca gcgcgctcga
     3901 tgtggcaatc accggcggga ccgaagccgc gattacgcag atgggactgg ccggcttcgc
     3961 gaacatgaag gccctctcga tgcggaacga cgacccgccg cgggccagcc ggccgttcga
     4021 ttccgagcgg gacggcttcg tgctcagcga aggggccggc ctcttgatcc tggaagagct
     4081 ggagcacgcg cagcagcgcg gagcacgcat ctactgcgag gtcgtcggct tcggcgctag
     4141 cgccgatgcc ggacacatca cgcagcccga cgagcagggc gcgggcgcgg gccgcgccat
     4201 gagcgacgcc ctggccgacg cgcagctgaa tgccaccgcg atcggctaca tcaacgccca
     4261 cggcaccagc acgccgctgg gagacgcggc cgagacgcag gccatcaagc gggtcttcgg
     4321 cgaccacgcc agggcggtca gcatcagcag caccaagagc cagctcggcc attcgctcgg
     4381 cgccagcggc ggcatcgagc tggtcgtcac ggtcaaggcc ctggctagca gcgccatccc
     4441 gccgacgatc aacctgacca gtcccgatcc ggcgtgcgac ctcgattaca cgcccaacac
     4501 tgcccgcgag cgccgcttca cgcacgccat gagcaacagc ttcggcttcg gcggccacaa
     4561 cgcctcgatc gtgatcgcca agctgtaggg aacgccctcc gtggcgttcc tcggctaagt
     4621 aaggtgtgac aaggccgccg cggccgccgg cacaccaatt tctgaaccgt tttccttgat
     4681 tccggtgtgc ctgcgctcgc ggactcgctg gtcacaccct acgcgttgct agcactcacc
     4741 gctcttgagc gccgacggca gcgcagttac aagtcgcccg ccgaattgcg tttgcacttc
     4801 gcgggaggcg ttcatgccgg cgaaatcctg gttcgtggcg atcttgcttg ctggtgcggc
     4861 tgcgccgctg ggcgcggcgg atgagtttcc ctacacggct tacgtcgcgc tcgagggcgc
     4921 ggaagtcgtc gctgggccag ggcaccgcta ctacgccacc gagcacctgc cgcgcggcac
     4981 gccggtcgaa gtttatcgcg aggaggcctc cggctggctg gcgatccgcc cgccggattc
     5041 atcgttcagc tgggtgcccg cgaaatcggt cgagcgcgcg ccggacgaca ggagcgccgg
     5101 ccgcgtgacc gggccgacgc cagcctgggt cggcacctcg gtcgagcacg ttggcgagca
     5161 ccggcagcag gtgacgctca aagagggtga gctgctgcag attatcggtg agaaggaagt
     5221 ggccgccgat ggggcggaga aggaggtctg gctcaagatc gcgccgccgg cgggagaatt
     5281 tcgctggatt cacctccgcg acgtcagccg gcaagcgccg ccgccggcct tgcccgagcc
     5341 ggagccggaa gtggacgagc agccgcagcg gcctgagccg gctgagcccc ggcggttgga
     5401 cgtcgcgggc ccgccgattg cgttggccga tattgagcgg cccaagtccc gccagctcgc
     5461 gctcgtggaa cgggcccagt ttcagaattc aggcgggtta tcgaatccgc tctcacccga
     5521 cggctttgtg ccccgcaagc ggacgggcag cgagcacctg cagccgccgg ccgtgccccc
     5581 ggcaggtttg acgcgcacct cgactcccac catccgatcg cgcctggcga gcgagtcgcc
     5641 gcgagaacct gccgcgcccg cgcccgtttc ggcgagcggc ggcgctggtg acctggccgc
     5701 gcagctggag cggctcgaac tcgacctctc gctgatggtc gccaaggaca agacgctgtg
     5761 ggacctggcc tcgatcaaga cccgcgtccg cgaccttgtg gaccacgggc ccgacccggc
     5821 gggccgcggc cgggcccgtt tgctgctgga caaagtggag cagttcgagc aggccttcgc
     5881 cgtgcagaat tacggcccga tccgcaccgg tgcgaccgcc gcggccgcga agtcgaagcc
     5941 tgcctcgccg ggaatggaag atccgcggta cgacgcccag ggctggctca agcccgtcat
     6001 ctcgcgccag ggagacaagc cggtcgctcc gtatgccgtg gtggatcagg acgggcagcc
     6061 gctgtgcttc atcacgccgc agccgggcat caacctgcgg cggtacgaga acaagcacgt
     6121 gggcgtttac ggccgccgcg gctttctcga agagctgaag aagccgcacg tcacggccga
     6181 gcggatcatc gacgtcgatc gccagtggcg ttagtaggcc ccttctgccg gaaggggcct
     6241 ttgccggagc gcagcgacca ggaggagccg aggattcgac cttatcgcgc tgtccaggtc
     6301 ccgcccggca ggcgggacct acaagtcagt cgtccgaaat gcggaatgct ttaggggcga
     6361 ccagccgttg gggctcgccc ggcgtctcgc ggctggtatt gggcaagggc gtgggcatgg
     6421 caggcgcttg aggcggcatc agttcaagcg gcgcggggcg ggcggcgggg cggctggcgg
     6481 taagggagcg gcgatttcct gcgccggcgg cgcgggagcg gcagcgagcc gcagttcctc
     6541 gggagccggc gaatcgagcc gcagcaccga atcgcagtcc ccaatcagcc gggcggcgtc
     6601 gatctgccgg ctgtccacgg gccagtcgag cagccgctga gccagggccc gcgcggcatc
     6661 gcgatccagg gcgctggtct gcggagcgtg cctggccagc aggctggcca gcgccgcgac
     6721 gcgcggcgcg gcctcttcgg gagtcagttg cgaccagcgg ttggcggcgg attgcacgcc
     6781 cagtcttccg cttctagcga cttggggccg cggatcggcc caggcacggg cgacaatctc
     6841 cagccgctgg tcgtcctcgg ccaacagcga gaccagccgg gccgcctggc gctccggcag
     6901 cgcggcgatt tgcgcctggt agcgttgctg cgagacttgc agggctgcgc tctggcgggc
     6961 ctgccaaatc cgcacagcgc cggccgcgac caggacggac gccgcgagca cgccgagcca
     7021 ccaggatgtc gttcgcgccc gcatggagtt gctccccggc caatggaagg cgcggattgt
     7081 cgccaagttg gcctgcggct caagagcagc tgcctgctag cggccggcgt tttcggcaag
     7141 gttttcctgg tccctccagc cggcggaagc cgaagatttc cacagaccgg caaagcttgc
     7201 cggcgatagt gattgcgaag ggcaccacag tgcgcgcgga ccgagggggg agcatgggca
     7261 gggaagagac gctgcgcgcg ggtccgccaa acgtgctc
//
LOCUS       DASRJB010000445         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3505944, whole genome shotgun sequence.
ACCESSION   DASRJB010000445 DASRJB010000000
VERSION     DASRJB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505944"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2345
                     /locus_tag="VFB80_11735"
     CDS             <1..2345
                     /locus_tag="VFB80_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZN34487.1"
                     /translation="VRLAAASAQPAARNTAQPAPKAATQAPVRNAAPPATPAPAPAAA
                     KPRPAGPPSPDQIRAKVLGGFQGTIERVPTTPLYKLGIVATALVMVLLPLIYIGIIAL
                     VCLLVWWHLTNNIAIVGAVRGRAAIVALMIYLAPAIVGAILIAFMVKPIFARASGDRR
                     RRSLTHKSDPLLFEFVERICSLVGAAQPRRIDIDCDINASASFRNGWLSLIGGRDLVL
                     TIGMPLAAGLSLQQFAGVLAHEFGHFSQGAGMRLTYIIRSINFWFMRAVYERDSWDDW
                     LAGAATEADLRIGWILYLAMGCVWVTRKILWLLMYAGHLVAGFMLRQMEFDADKYEAR
                     VAGSETFAATARQLKLLGVAWHGAQADLASYHREGRLADNLPRLLMANLKQLPKAAHE
                     YVAKQIAETETGSFDSHPADKDRIESARAEQSAGVFRSDLPATVLFDGFDQTAKNVTW
                     DYYRSIFGPTVKQNALHNTDELLARTENEQAASKAANRFFAGAFNVLRPLRLPVMQGQ
                     TTQHPSVWKAELAETRAAMESQAESYRQTLEAFDKADTRLVQARQARSVLSCGVQLLA
                     DKFDQPFTSDSHASRIRDGAQTEIARCGNRMEAFEESAGRRLRAGLLLLFDPATAVRL
                     SETEAMQQECRCLLPIANQVANNHAGILELRNNNATLVALLGHLDGQERNESLIREIL
                     DFGGRVRKQLADLKQQFERVDYPFDHAEGKMSIGRWLVQIVPPEEEVGAVFEAGDQVL
                     NKLLELYARALSRLCVIAEAVEATFGYSPLPVPDKSESSQ"
     gene            2487..>2865
                     /locus_tag="VFB80_11740"
     CDS             2487..>2865
                     /locus_tag="VFB80_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34488.1"
                     /translation="MPIAAQCPHCGENMQVREKYAGKKGMCPKCQRPFRVPESSDQRA
                     AEPSEARTVASRTLTGRTGGPTAPKEGVIEVDASAAHLVAVVPTNPGELREAILGGFD
                     SRITPPQVPLGRKLFTQIVLGVLL"
BASE COUNT          501 a          968 c          929 g          467 t
ORIGIN      
        1 cggtgcggct ggccgctgca tcagcgcagc ccgccgcaag aaatacagcc cagcctgcgc
       61 cgaaagccgc aacgcaggcc cccgtccgaa atgccgcgcc accagcgacg ccggcacccg
      121 ctccggccgc cgccaaaccg cggccggctg gaccgccttc gcccgaccag attcgggcca
      181 aagtcctcgg cggctttcaa ggcacgattg agcgcgtccc tactacgccg ctttacaagc
      241 tgggcatcgt ggcaacggcc ctggtcatgg tcctcttgcc tctcatctat atcggcatca
      301 ttgctctggt gtgcctgctg gtctggtggc acttgaccaa caatattgcc atcgtcggcg
      361 cggtccgcgg ccgggccgcg atcgtggccc tgatgattta tctcgccccg gcgatcgtcg
      421 gcgcgatctt gattgcgttc atggtcaagc cgatcttcgc ccgggccagc ggcgaccggc
      481 gccgccgcag cctgacgcac aagagcgacc cgctgctctt cgaattcgtc gagcggatct
      541 gctccctggt cggcgctgcc cagccgcggc ggatcgacat cgactgcgac atcaacgcct
      601 ccgccagctt ccgcaacggc tggctgagcc tgatcggcgg ccgcgacctg gtgctgacga
      661 tcggcatgcc cctggccgcg ggcctgagct tgcagcagtt cgcaggcgtc ctggctcacg
      721 aattcggcca cttcagccag ggagccggca tgcggctgac gtacatcatc cgctcgatca
      781 acttctggtt catgcgggcg gtttacgagc gggactcgtg ggacgactgg ctcgccgggg
      841 ccgccacgga agccgacctg cggatcggct ggattctgta cctggcgatg ggctgcgtct
      901 gggtcacgcg caagatcctg tggctgctga tgtatgctgg gcatctggtc gccggcttca
      961 tgctgcggca gatggagttc gacgccgata agtacgaggc ccgcgttgcc ggcagcgaga
     1021 cctttgccgc caccgcgcgg cagctgaagc tgttgggcgt cgcctggcac ggcgcgcagg
     1081 ccgacctggc ctcgtaccac cgcgaaggcc gcctggccga caacctgccg cggctcttga
     1141 tggcgaacct caagcagtta cctaaggcgg ctcacgagta cgtcgcgaag cagatcgccg
     1201 aaaccgagac gggaagtttc gattcgcatc ctgccgacaa ggaccgcatc gaatcggccc
     1261 gggccgagca gtccgccggc gtctttcgca gcgacctgcc cgcgaccgtg ctgttcgacg
     1321 gcttcgacca gacggccaag aacgtgacct gggactatta ccgctcgatc ttcgggccga
     1381 ccgtcaagca gaacgccctc cataacaccg acgagctgct ggcccgcacc gaaaacgagc
     1441 aggcggcgtc caaggcggcg aaccggttct ttgccggggc gttcaacgtc ctgaggccgc
     1501 tgcgattgcc ggtgatgcag ggccagacga cgcagcatcc gtcggtctgg aaagcagagt
     1561 tggccgagac tcgcgccgcg atggagtcgc aggccgagag ctaccggcag acgctggagg
     1621 cgttcgacaa ggccgacact cggctggtgc aggcccggca ggcccgcagc gtactgtcgt
     1681 gcggtgtgca gctcttggcc gacaaattcg accagccgtt cacgtcggat tcgcacgcct
     1741 cgcggattcg cgacggcgcc cagacggaaa tcgcccgctg cggcaaccgg atggaggcct
     1801 ttgaagagtc agccggccga cggctgcggg ctggcttgct gctcttgttc gatccggcca
     1861 cggccgtacg gctttccgaa accgaggcca tgcagcagga gtgccgctgc ctattgccga
     1921 tcgccaacca ggtcgccaac aaccacgccg gaatcctgga gctgcgcaac aacaacgcca
     1981 cgctggtcgc gctgctgggt catctcgacg gccaggagcg caatgaatcg ctgatccgcg
     2041 aaatcctcga tttcggcggc cgcgtccgga agcagctggc cgatctcaag cagcagttcg
     2101 agcgggtcga ttatccgttc gaccatgccg aggggaaaat gtcgatcggc cgctggctgg
     2161 ttcaaatcgt tccgccggag gaagaggtcg gggcggtctt cgaggccggc gatcaggtgc
     2221 tcaacaagct gctggagctc tacgcccggg ctctcagccg actgtgcgtc atcgccgagg
     2281 ccgtcgaggc cactttcggc tattcgccgc tgccagtgcc cgacaagagc gagtccagcc
     2341 agtaaggctg cacagatcgc agtgggggaa ttagactatg agccgttgcc aacgcgcagc
     2401 ccgccgtcgg aaagacaggg cggcccgccg acagaaatgc agcgcagttc gccgcccgcc
     2461 atccggcgca ccttgaggaa ttcttcgtgc caatcgcagc tcaatgcccg cactgcggcg
     2521 aaaacatgca ggtgagggaa aagtacgccg gcaagaaggg catgtgcccg aaatgccagc
     2581 ggccctttcg cgtgcccgaa agctccgatc agcgggcagc ggaaccttcc gaggcccgca
     2641 cggtggcttc ccgcacgttg accggccgca cgggcggccc caccgcgccg aaggaaggcg
     2701 tgatcgaggt ggacgcatcg gcggcccacc tcgtggccgt cgtgcccacc aatccgggcg
     2761 agctgcgcga ggcaatcctc ggcggattcg acagccgcat cacgccgccg caagtgccct
     2821 tgggccgcaa gctgttcaca cagatcgtgc tgggcgtgct gctgg
//
LOCUS       DASRJB010000446         6207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2380721, whole genome shotgun sequence.
ACCESSION   DASRJB010000446 DASRJB010000000
VERSION     DASRJB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380721"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VFB80_11745"
     CDS             complement(<1..1265)
                     /locus_tag="VFB80_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN34489.1"
                     /translation="MARKSDFTEILLRKRIISQDQLNEAKQVSREQNTSLPDTIIKLG
                     YASGEDVMRALAQEHGRDYVDLTEVTIQESIIELVPESVARENVTLPFAEEEDALKVI
                     VSDPYDIDTVEKLRFILNRRIEIALAPRDKILEAINKYYSQIEGESADSVLQEFTDTA
                     IDFTETETVSTAVGPGEVVDENSAPIVRLVQLMITEAVQLRASDIHVEPFEEEVRIRY
                     RIDGMLHKRDSPPRRLLGAIISRIKILAKMDIAERRRPQDGRIKITVGDKELDLRVSI
                     IPTNHGQSAVMRLLDKDNIKVGVRQLGLSDDNFQQFAQLIRRPNGIILVTGPTGSGKT
                     TTLYAALNALNRPDRKIITAEDPVEYYLPGINQVEVRHSIGLDFSRIIRAMLRQAPNI
                     ILVGEMRDDETASMGIQASLTGHLVFSTL"
     gene            complement(1537..2250)
                     /locus_tag="VFB80_11750"
     CDS             complement(1537..2250)
                     /locus_tag="VFB80_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34490.1"
                     /translation="MGKVLSGICCVIVGLEVLIGVPVAVCLSFIVLTQGGPVVQEFHA
                     GPIPSTIPPPPFAGPVSYPPAIVPPLPPAPPSYSRVAASPEGLEMPAVKEAPVAKEIS
                     AHDNPVMTAITEARERIGNPLAGTVLAPAADEPSQAEFVQSLRQLDESAASLPTLADA
                     IQPPAIVEQPADDAVAALKQSVERLYQLAGRFEDEGKFGQADRLRSLARSVREELATM
                     GKGSAPAEEPPIALAPYRP"
     gene            complement(2407..2943)
                     /locus_tag="VFB80_11755"
     CDS             complement(2407..2943)
                     /locus_tag="VFB80_11755"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HZN34491.1"
                     /translation="MTGGIRMGLVAAGLLGIALGIGVWAGAAAQEKKPEDAAVARTRK
                     QVLMLDDLYKNAIVLITENYVQEDSDLAAGAAFQKLFEAMRKKGHHDVRLLDATGEPY
                     NAKNLPRDEFEKAAVAALKSGKANYEQITEQNGKRVLRLATPIPVVMKKCTLCHPAYE
                     KAAAGQPIGALGYTIAIE"
     gene            complement(2990..3478)
                     /locus_tag="VFB80_11760"
     CDS             complement(2990..3478)
                     /locus_tag="VFB80_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZN34492.1"
                     /translation="MRLALHTDYALRTLLYLAGNPGRASVGQVAEFFRISRDHVAKVV
                     QALVRHGYVRSIRGLGGGIELARRPEEIVIGRVILDFEGHLHLLECVGVENVCTIQPG
                     CRLRSVLAHAERLQVEYLNSVRLTEVVRPGGKLLEITRPDQTPAAVPRIEGKESKKKP
                     KT"
     gene            3653..4540
                     /locus_tag="VFB80_11765"
     CDS             3653..4540
                     /locus_tag="VFB80_11765"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN34493.1"
                     /translation="MALEDRDYYREADRYGGGGAAGAALQTVVARLILINGALFLANA
                     FFGGQDNQITRALMLHSDAIVHPWMWYQFLTAGFVHDWENLLHIVGNMLGLFVLGREL
                     EQKLGGREFLLFYLASIVFGSVVWSVRNHFFAGPDASGSCLGASGGVTAVIILFCLQN
                     PRAMLGLVPIPAWVFGLLVVATDIFGSSPAALGSRVAYDVHLAGAAFALAYWAFGWSF
                     GKLPGISQIARLLKSPRKWLEPKPPLRVHDPEQYYEELDEQADRLLEKVNREGLESLN
                     AQERQLLEDYSRRTRQKLR"
     gene            complement(4525..5400)
                     /locus_tag="VFB80_11770"
     CDS             complement(4525..5400)
                     /locus_tag="VFB80_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN34494.1"
                     /translation="MFARLADRFVLRPSRNAIPASHKTRHTVRVEGRQVEVWIERTDG
                     RAAPTIIPSVIGQPAPDLPDGNGRPDGERAPLPEPQIFVLKFNGAGGRAERASIHPLD
                     FWSDVPGEIWSANPPGYGASEGRPSLQWLAPTGRAVLEELVKVAGSRPIVLTGNSLGT
                     TVALRLAAEFAAVSNLAALLLRNPPPLRQLIAGKFGWRTLGLSLGVARQIPPDLDSVT
                     NAARVKLPCVFMCAGCDRVVPPRFQQLIVNAYAGPKQVVTIPDADHVFMLSEPQLTEY
                     GTALTWLRENSYRSF"
     gene            complement(5416..>6207)
                     /gene="metG"
                     /locus_tag="VFB80_11775"
     CDS             complement(5416..>6207)
                     /gene="metG"
                     /locus_tag="VFB80_11775"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HZN34495.1"
                     /translation="GTEIAAAYEACDYARAMRLIMELADRANPYIDAQKPWVLAKQPG
                     SEAELQNVCTVGLNLFRQIIVYLSPVLPKLAEQAGALLNDPIKSWSQSQQPLVGTPVA
                     PFSHMMKRAETKDLDAMIEESKPQESGVASQESAAAAPADSDAALQAEPLLTDLISIE
                     EFSKVDLRVARVLAAEEVPGAKKLLKLTLGLGGDLKRQVFAGIKAAYKPEALVGRLVV
                     VVANLQPRQMKFGLSEGMVTAVGPGEENVFLLALDAGAQPGMRVH"
BASE COUNT         1050 a         1997 c         1963 g         1197 t
ORIGIN      
        1 tgcaatgtac taaaaactaa gtgtccagtc aaagaagctt gaatgcccat gcttgccgtc
       61 tcgtcatcgc gcatctcgcc gacgagaatg atgttgggtg cctgccgcaa catggcacgg
      121 atgattcgcg agaaatccag cccaatgctg tgccggacct ccacctggtt aatgccgggg
      181 aggtaatact caaccgggtc ctcggccgtg atgatcttgc ggtcggggcg attcagggcg
      241 tttagcgcgg catagagcgt cgttgtcttg ccggagccgg tcggcccggt gaccaggatg
      301 atcccgttgg ggcggcggat aagctgggca aactgctgaa agttgtcgtc cgagagcccc
      361 aactggcgca cgccgacctt aatgttgtcc ttgtcgagca gtcgcatcac cgccgactgg
      421 ccgtgattcg tcggaataat acttacacga aggtccaact ccttatcgcc gaccgtgatc
      481 ttgatccgcc cgtcttgggg gcggcgtcgc tcggcgatgt ccattttcgc cagaatcttg
      541 atgcgcgaaa tgatagcccc caggagccgg cgaggcgggc tgtcccgctt gtggagcatt
      601 ccgtcgatcc ggtagcggat gcggacttcc tcctcgaagg gctcgacgtg gatgtccgac
      661 gcccgcagct gcacggcttc tgtgatcatc aactgaacca ggcggacgat cggggcactg
      721 ttctcgtcca ccacttcgcc tgggccgacc gcagtgctta cggtctcggt ttccgtaaag
      781 tcaatggccg tgtcggtgaa ttcctggagc acggagtcgg ccgattcgcc ttcgatctgg
      841 ctgtagtact tgttgatggc ctccaggatc ttgtcgcgag gagcgagggc gatttcgatc
      901 cggcggttaa gaataaaccg caacttttcg acggtgtcga tgtcgtaggg gtcgctgacg
      961 atgaccttca gggcatcttc ttcctcagcg aagggcaaag tcacgttttc gcgggccacc
     1021 gattcgggca ccaattcgat gatcgattcc tggatggtga cttcggtcaa gtccacgtaa
     1081 tcgcggccgt gctcctgggc caaggctcgc atgacgtcct cgcccgaggc atagcccagc
     1141 ttgatgatcg tatcgggcag actcgtgttc tgttcccggg agacctgctt ggcctcgttc
     1201 agctggtcct ggctgatgat ccgcttgcga agcaggattt cggtgaagtc gcttttgcga
     1261 gccacggatg gcggtccttt caataagcag ccgcagcgga tcgcccgccg atcatggatc
     1321 agcgcaggca ccgcatcggc tgcgaacctt ggcccctgtt ggcctcttct acttacggcg
     1381 gttgggcgct gaacgagccc agggaatccg ccccaagggg acgaagcgca gcaccggcac
     1441 cacaaaattg ccgctagtgc ctattttgag agctttgccg ccaatgtcaa ttcattgcgg
     1501 caggcgacgg caagaaacct cacgccccct aatgggctat ggtcgatatg gcgccagcgc
     1561 aatgggtggt tcctcggccg gagccgatcc cttgcccatg gttgccaatt cttcacgcac
     1621 gctgcgggcc agcgagcgca gacggtcggc ctggccgaac ttgccttcgt cttcaaaacg
     1681 gccagcgagt tgatagagcc gctccaccga ttgcttcaag gcggccacgg catcgtcggc
     1741 cggctgctcg acaatggccg gcggctgaat cgcgtcagcc agcgtgggca ggctggcagc
     1801 actttcgtcc aattgacgca gcgactggac gaactccgcc tgcgagggct cgtcggccgc
     1861 gggcgctaga accgtcccgg ccaggggatt gccaatgcgc tctcgcgctt cagtgatcgc
     1921 cgtcatcacg gggttatcgt gagcggagat ttctttggcg accggcgctt ccttgacggc
     1981 aggcatctcc aaaccctcgg gcgaagccgc cacgcgtgaa taagagggcg gagctggcgg
     2041 cagcggagga acgatcgccg gagggtagct caccgggccg gcaaacggcg gcggcggaat
     2101 tgtcgagggg atgggcccgg cgtgaaattc ctggaccaca ggcccgccct gcgtgagcac
     2161 aatgaacgac aggcataccg ctaccggcac gccaatcaac acttccaggc ccacgatcac
     2221 acagcagatt ccagacagca ccttgcccat tgcaacctcc ttgccgcagg tcttccactc
     2281 ctctagccga cgctggcagc gtcggccagg cggtgattat aggtcgcgtg cttctgcgcg
     2341 accaagagca ttgcactgcc gacgctggca gcgtcggcta gcgagttttc cgctcgcaga
     2401 tgcggcctat tcgatcgcga tcgtatatcc gagcgcgccg atgggttgcc cggcagcggc
     2461 cttctcatag gccggatgac agagcgtgca cttcttcatg acgaccggga tcggcgtcgc
     2521 caggcgcagc acgcgcttgc cgttctgctc ggtgatctgc tcgtagttgg ccttgccgct
     2581 cttgagcgcg gcgacggcgg ccttctcgaa ttcgtcgcgc ggcaggtttt tggcgttgta
     2641 cggctcgccg gtagcgtcga gcagccgcac gtcgtgatgg cccttcttcc gcatggcctc
     2701 gaagagcttc tgaaacgccg cgccggcggc gagatccgaa tcttcctgca cgtagttctc
     2761 ggtgatcagc acgatcgcgt tcttatacaa gtcgtcgagc atcagcacct gcttccgcgt
     2821 gcgggcgacc gccgcatcct ccggtttctt ctcctgcgcc gccgcacccg cccaaactcc
     2881 aatgccaagt gcgatgccca gcaggcccgc cgcgaccaac cccattcgaa ttccgcccgt
     2941 cattacattc gctcctctga aatgaagtga aacgcaaccc cacccgcgat catgtcttgg
     3001 gtttcttctt ggattctttg ccctcgatgc gaggcacggc ggccggcgtc tggtccggcc
     3061 gcgtgatctc cagcagcttt ccaccggggc ggacaacctc ggtcagccgc acgctgttca
     3121 aatactcgac ctggagccgc tcggcgtgag ccagaaccga gcgcaagcgg cagccgggct
     3181 ggatcgtgca aacgttttcg acgccgacgc attccagcag gtgcaggtgg ccttcgaagt
     3241 cgaggatgac ccggccgatt acgatctcct cggggcggcg ggccaattcg atgccgcccc
     3301 ccagtccgcg aatgctccgc acgtagccgt ggcggaccag agcctggacg actttggcca
     3361 cgtggtcgcg ggaaatgcgg aagaactcgg ccacctggcc gacgctggcc cggccgggat
     3421 tgcccgccag gtagagcagc gtacgcaggg cgtaatcggt atggagggcc agccgcatgg
     3481 cgaagaggta taataggtac gtcttatacc aattttagag cttagcggcc gcagcgggcc
     3541 tgtcaagcgg aatggcggca ctctatcctt tgccctcccc gctcccccgc ctgcggcgta
     3601 atattcaagc gaccgcggca gcggcggtta cgcgtgcaga ccaaggcaga aaatggccct
     3661 cgaggaccgg gactattatc gcgaagcaga ccgctatggc ggcggcggag cggctggcgc
     3721 tgcgctgcaa acggtcgtgg cccggctaat cctcatcaac ggggccttgt tcctggccaa
     3781 cgccttcttc ggcgggcagg acaaccagat cacgcgggcc ctgatgctcc acagcgacgc
     3841 catcgtgcat ccctggatgt ggtatcagtt tctgaccgcc ggcttcgtgc acgactggga
     3901 gaacctgctg cacatcgtgg gcaacatgct cggcctgttc gtcctgggcc gcgaattgga
     3961 gcagaagctg gggggccgcg aattcctgct gttctatctg gcgtcgatcg tcttcggctc
     4021 cgtcgtctgg agcgtgcgga accatttctt cgccggtcca gatgcgtccg gttcctgcct
     4081 gggcgcgtcg ggcggcgtga cggcagtgat catcttgttc tgcctgcaga acccgcgggc
     4141 catgctgggc ctggttccca ttccggcgtg ggtcttcggc ctgctggtcg tcgcgaccga
     4201 tattttcggc tcatcgccgg cggcgctcgg ttcgcgcgtg gcctacgacg tgcacctggc
     4261 cggcgcggcg ttcgccctgg cctactgggc tttcggctgg agcttcggca agctgccggg
     4321 catttcccag atcgcgcgcc tgctcaagtc gccgcgcaag tggctcgagc ccaagccgcc
     4381 gctccgggtc cacgatcccg agcagtacta cgaagagctc gacgagcagg ccgaccggct
     4441 gctggaaaag gttaaccgcg agggcctgga gagcctgaac gctcaggagc ggcagctgct
     4501 cgaggactac agccgcagga cgcgtcagaa gctgcggtag gagttctccc gcagccaggt
     4561 cagtgccgtg ccgtactctg tcagctgcgg ctcgctgagc atgaagacgt ggtcggcatc
     4621 gggaatcgtc acgacctgct tggggccggc gtaagcgttc acgatcagct gctggaaccg
     4681 cggcggcacg acgcggtcac agcccgcgca catgaaaacg cacggcagct tgacgcgggc
     4741 agcgttggtc acgctgtcga gatcgggggg aatctgccgg gcgacgccga gcgacagtcc
     4801 cagcgtgcgc cagccgaact tgccggcaat cagctgccgc aggggcggtg gattgcggag
     4861 cagcagtgcg gcgaggttgg agacggcagc gaattccgcg gccaatcgca gcgctaccgt
     4921 cgttccgagg ctgtttccgg tgaggacgat cggccgcgag ccggctactt tcactaactc
     4981 ctccagcacg gcccgcccgg tcggcgcgag ccattgcaga ctcggacgcc cttcgctggc
     5041 cccatagcca ggcgggttcg ccgaccagat ttcgccgggc acgtcgctcc agaaatcgag
     5101 cggatgaatg ctcgcccgct cggcccggcc gccggcgccg ttgaatttca ggacgaaaat
     5161 ctgcggctcc ggcagcggcg cacgctcgcc atccggccgt ccgtttccat ccggcaggtc
     5221 tggcgccggc tgcccgatca cgctcggaat gatcgtcggc gcagcccgcc cgtcggtccg
     5281 ttcgatccag acctcgactt gccgcccttc cacgcgcacg gtatgccgcg tcttatggct
     5341 ggccgggatc gcgttccgcg acggcctcag cacaaaccgg tcggcaagac gggcgaacat
     5401 ccagcgatcc cctggctagt ggacgcgcat gcccggctgc gcgccagcgt cgagggcgag
     5461 caagaagacg ttttcttcgc ctgggccgac tgccgtcacc atgccttcgg agaggccgaa
     5521 cttcatctgc cgcggctgca gattggcgac cacgacgacg aggcggccga ccagtgcttc
     5581 gggcttgtac gccgctttga tgcccgcgaa gacctgccgt ttcaaatcgc cgcccaggcc
     5641 cagcgtcagc ttcagcagct ttttcgcccc cggcacttct tctgccgcga gcacgcgggc
     5701 cacccgcaga tcgaccttgc tgaattcttc gatcgagatc aggtccgtca gcagcggctc
     5761 agcctgcagc gcggcgtcgg aatcggcagg agctgccgct gctgactcct gactcgcgac
     5821 tcctgactcc tgcggcttgc tttcctcaat catggcatcg aggtcctttg tctcggctcg
     5881 tttcatcata tggctgaacg gtgcgaccgg tgtgccaacg agcggctgct gggactggct
     5941 ccacgacttg atcgggtcgt tcagcagcgc gccggcctgc tcggccagtt tcggcagcac
     6001 gggcgacaga tacacgatga tctggcggaa caggttcagc ccgaccgtgc agacgttttg
     6061 caactccgcc tcgctgcccg gttgcttggc gagcacccag ggcttctggg cgtcgatgta
     6121 cgggttggcc cggtcggcca gctccatgat cagccgcatg gcccgggcgt agtcgcaggc
     6181 ttcgtaggcc gcggcgatct cggtccc
//
LOCUS       DASRJB010000447         9395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_974809, whole genome shotgun sequence.
ACCESSION   DASRJB010000447 DASRJB010000000
VERSION     DASRJB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974809"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFB80_11780"
     CDS             complement(<1..496)
                     /locus_tag="VFB80_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN34496.1"
                     /translation="MSSAAQLRRAIDDKTARVGIVGLGYVGLPLARTFVAAGFRVLGF
                     DVDAGKVEKLRRGESYIGHIPGEWIKECIAAGRFEPTADMALLREPDAILICVPTPLS
                     ANRDPDLSYVEQTARQIAAAIRPGQLIVLESTTYPGTTRDVVLPILLASGKRKLPDHS
                     QSPEA"
     gene            672..1223
                     /locus_tag="VFB80_11785"
     CDS             672..1223
                     /locus_tag="VFB80_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZN34497.1"
                     /translation="MTHPWHDVTPGLMLPQEFMAVIEIPLGSSVKYELDKATGLLRVD
                     RILYSAVYYPANYGFIPQTLAEDDDPLDVLVLCQEAVDPLTLVRARVIGLMTMVDSGK
                     RDHKVLAVAMDDPEFNSFREASELPIHRLTMLRRFFQDYKQLEGKSVEVDDFQPAAFA
                     LPIIEDALQRYSQQRRQGFGSKK"
     gene            1323..4172
                     /locus_tag="VFB80_11790"
     CDS             1323..4172
                     /locus_tag="VFB80_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34498.1"
                     /translation="MSTLVDSSLIHWLAALGAGALRRQAGAATEEMRQAQAAFMSLVD
                     SLPLCLVIKDASGRRVLANKSYLAAHNKTPAEVLGKTDAEIFPPEIARKFSADDEEVL
                     RTGQLHHAVEEHQVEGGQRRWIERLKGPLRDADGNIVGIQVLFWDVTDREHAEKALDR
                     EQYLLHALMDNVPDSVYFKDRDSRFLRISRAQARRFGLVSPDEAIGKSDADIFTPEHA
                     QEALANEREIMRTGQPMVARIQRLTWPDRPDTWVSSTKMPLRDNEGQIVGTFGISRDI
                     TELKRAELELEKARKAAEDASRAKSEFLANMSHEIRTPMNGIIGMTELALDTELTPEQ
                     REYLSMAKSSADYLLTVINDILDFSKIEAGKLEIENIDFALRDCVEETAATLALRAHK
                     KGLELACHVHQDVPDALIGDPGRLRQILVNLIGNAVKFTERGEVVVEVRSQEPVTRGQ
                     GSPTSGPWPLAPGSYFLQFSVRDTGIGIPPEKAGLLFQAFSQVDSSTTRKYGGTGLGL
                     AISAQLAQLMGGRAWVESEVGRGSTFHFTAQFGIATSPPPALPVEAVRLSDLPVLVVD
                     DNATNRRILKEMLTNWGLRPTVVPGAREALAALDAARCAGRAFPLILLDGMMPEMDGF
                     ELAARIKQQPDLVGASLMMLSSADRREDAARCKQLGVATYLVKPVRQSDLLDAIVNTL
                     AANASQSAPVDAEPPPAKQPAVLQAERRLNLLLAEDNAVNQRVAVRLLEKRGHEVTVV
                     GTGRQAVAAALAQPFDAILMDVQMPELDGFEATAAIRAAEHMHGGRRKIIAMTAHAMK
                     GDRERCLQAGMDGYVSKPLQPEVLFAAIERPAAGGESREGPTPLDEAAISKHFGDEEF
                     FREVAKVFLESCPDWLADLRAAVTARDAKRVLVAAHTLKGAVSHFGAQAVYDAAHQLE
                     QMGKTQQLDDAPPALAALGRALDGLQAALRERSKV"
     gene            4180..5232
                     /gene="rlmN"
                     /locus_tag="VFB80_11795"
     CDS             4180..5232
                     /gene="rlmN"
                     /locus_tag="VFB80_11795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZN34499.1"
                     /translation="MPSSLLDLPRSSLARLLADWGFSAAHGDRLWRYLYREAKADFAA
                     MEELPRRLRERLLASANIDAPSVLRETVADGGLTTKHLLELADGQRIETVLMRPSGRV
                     TACLSSQAGCPLGCVFCATGQMGFGRNLSAGEIVAQAVHVQRRLGPATRLRNVVLMGM
                     GEPLLNYESVLRAADILGDGGGLAIAGKRLTLSTVGVVPGIVRLADEGRTCSLAVSLH
                     AATQEERAALVPAAATWPLADLMRACRYYAAKLGRRIFFEWTLIAGRSDSPEQALELA
                     RLVRGLPAHINLIPLNPTAGYAAAPAEPEAVRRFQAALREQGLPCTIRRRRGIAIAAG
                     CGQLVVADAASALAPA"
     gene            complement(5229..5960)
                     /locus_tag="VFB80_11800"
     CDS             complement(5229..5960)
                     /locus_tag="VFB80_11800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34500.1"
                     /translation="MHRVLAASLACLPLVAAGCADGPVPELRTLNPWVRRQWDADERA
                     IATYHRKVADLAALRAQAARLPAEQAEPLAAQLAAGLQAETAPVLRAELARTLGELPT
                     PAARSALQAALTDEAPNVRIAACQALARHPAPEAVEPLAHAVSADADLDVRIAAADAL
                     GKFAAAGLPAEEYKRLAQSLRPALDDRDPALQTAAMQSLAAITGRDNFRNDLNTWRQY
                     LDGANPAPPPPPTVAELVQRSLNWY"
     gene            6075..6764
                     /locus_tag="VFB80_11805"
     CDS             6075..6764
                     /locus_tag="VFB80_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN34501.1"
                     /translation="MTESPPPSASEPLLPAAGWTARRLVLAAAVLMVFAAAALPLDLA
                     AARFVQAHRLTGDLRRIVTLAEVFGWGGTIALIILTAVVLDPRGWRVAPRLAIGSLAA
                     GLAADGLKLLGVARWRPAPALEAGLNQTFVGWLPLWRHDPALGAYDSRLQSFPSGHAA
                     NAVGLAIALAVLYPRGRWLFAAFAVLACLQRVEGLAHFPSDVLVGAAIGCLASAICAG
                     PLHRRLTALEQ"
     gene            complement(6865..7932)
                     /locus_tag="VFB80_11810"
     CDS             complement(6865..7932)
                     /locus_tag="VFB80_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34502.1"
                     /translation="MSRRAALACLVALAGLHSAIGPAAAQRTVPPRQFAPGVVTTIPP
                     KPETEEMFSGPRPLVEVPVSIPGLEYDPKLAPKTSTIFERSKEATLRRTIWNLEFSFK
                     PMRMVHVDVPQASNRMQRQLVWYMVYRVRNLGSHITPIEEREEVKLESGEVGRVHVNF
                     KRGTTNDVELFGRKTTDLRFFPHFVLASLEYDKEYLDRVIPAALAPIKAREFPGRTDQ
                     LLYDSLSISEVPLKVSTEKEDNGVWGVVTWTDVDLRIDYFAIYIQGLTNAYRFEDPAG
                     AYKSGDPPGTGRQITKKSLQLNFWRPGDTVDPNEEEIRYGCRTDGTPEEQAKVLAEYG
                     IDKPIDHLWVYRSTLAPPPKK"
     gene            complement(8113..8517)
                     /locus_tag="VFB80_11815"
     CDS             complement(8113..8517)
                     /locus_tag="VFB80_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZN34503.1"
                     /translation="MPRFVVLRHETPPGYARPAHFDLMLEVGGVLRTWAALSLPAVGG
                     EPVAAERLPDHRLDYLNYEGPVSGDRGCVSRVDWGTYEPLPSAPNAPNQQRFRLEGTR
                     LRGCLVLAATGQRAENWQVQLLEDWSFNPEPG"
     gene            complement(8541..>9395)
                     /locus_tag="VFB80_11820"
     CDS             complement(8541..>9395)
                     /locus_tag="VFB80_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34504.1"
                     /translation="SHAERLRAQRWLTYERNELILASCLRIAGEMESTVDVTTRTLQR
                     PHSQPGADREVRTTFSAQDRHGLAARFLRLAYRINMDNLKLVQQDELPPLDPLSPEQL
                     AAEEAAAEQLRAERQSAKSNGLSYYDEQRERARQRQAELDEQDRQREEQFAAAARLKE
                     REEAERQALAEELLAQESASAAAAEETEVPSYQVSTNSPAASDSAPSAHKAHNDAPEN
                     HAATADRTTTYAKNGRPEKTARHARMNGRPAARKGKAPPARPAASGQAVAELRISPEL
                     AGAVAKSP"
BASE COUNT         1448 a         3239 c         3170 g         1538 t
ORIGIN      
        1 gcgcttccgg cgattgcgaa tgatcgggaa gcttacgctt cccgctcgcc agcaaaatcg
       61 gcaacaccac gtcgcgggtc gtgcccggat aagtcgtgct ctccagcacg atcagctgcc
      121 ccggccggat cgctgcggcg atctgccgcg cggtttgctc gacgtagctc aaatccggat
      181 cgcgattggc tgagagcggc gtcggcacgc aaatcaggat cgcgtcgggc tcgcggagca
      241 gcgccatatc cgcggtcggc tcgaaccggc cggctgcgat gcactccttg atccactcgc
      301 ccggaatatg gccgatatag ctctcgccgc gccgcagctt ctcgaccttc cccgcatcga
      361 cgtcgaaccc cagcacgcga aaccccgccg ccacgaacgt ccgcgccagg ggcaagccca
      421 cgtagcccaa gccgacgatg cccacgcggg ccgttttgtc gtcgatcgcg cggcgaagct
      481 gagcggcaga ggacataggg cgggagtcgg ggggtcggga gtcgggagtc gggagtcggg
      541 agtcgaagct gattagctaa cccatggtaa ctcaagcgtt gcggcacctg cggtgtattc
      601 tttgcactgg tacgatagag cggatcgaca agttcgagcc ctcgactcca cggacccccg
      661 actcccgccc catgacccat ccctggcacg acgtcacgcc cggcctcatg ctgccgcagg
      721 aattcatggc ggtgatcgaa atcccgctgg gcagcagcgt caagtacgag ctggacaaag
      781 cgaccggcct gctgcgggtg gaccgcatcc tgtattcggc cgtctattac ccggcgaatt
      841 acggcttcat tccccagacg ctggccgagg acgacgaccc gctggacgtg ctcgtgctct
      901 gccaggaggc ggtcgatccg ctgacgctgg tgcgggcccg cgtgatcggc ctgatgacaa
      961 tggtcgatag cggcaagagg gaccacaagg tgctcgccgt ggcgatggac gatccggagt
     1021 tcaacagttt tcgcgaggcc agcgagctgc cgatccaccg gctgacgatg ctgcggcggt
     1081 tcttccagga ctacaaacag ctcgaaggta agagcgtcga ggtggacgac tttcagccgg
     1141 ccgcctttgc cctcccgatc atcgaggacg ccctgcagcg ctacagccag cagcgccggc
     1201 agggttttgg ctcgaagaaa tagacccgcg tggcggtttg gggttgagct tgccgctggg
     1261 ctataatcgg cgtcagcaga agtcattgca atcgcggtcg ccttttcccg atcggccagg
     1321 caatgagtac tcttgtggat tcgtcgttga tccactggct ggcggcgctg ggcgccggcg
     1381 ctctgcgccg gcaggccggc gcggcgaccg aggagatgcg gcaggcccag gcggcgttca
     1441 tgtcgctggt cgatagcctg ccgctgtgcc tggtgatcaa ggacgcctcc ggccgccgcg
     1501 tgctggccaa caagagctac ctcgcggccc acaacaagac gccggccgaa gtcctcggca
     1561 agaccgacgc cgaaatcttc ccgccggaaa tcgcccgcaa gttctcggcg gacgacgaag
     1621 aggtgctccg taccgggcag ctccatcacg ccgtcgaaga acaccaggtc gaaggcggcc
     1681 agcggcgctg gatcgagcgc ctcaagggcc cgctccgcga cgccgacggc aacatcgtcg
     1741 gcatccaggt gctgttctgg gatgtgaccg accgcgagca cgccgagaag gccctcgacc
     1801 gcgagcagta tctgctgcat gccctgatgg acaatgttcc cgactcggtc tatttcaagg
     1861 accgcgacag ccgcttcctg cggatcagcc gggcgcaggc ccgccgcttc ggcctggtga
     1921 gcccggacga ggccatcggc aagtccgacg ccgatatctt cacgcccgag cacgcccagg
     1981 aagccctggc caacgaacgc gagatcatgc ggaccggcca gccgatggtc gcccgcatcc
     2041 agcggctgac ctggcccgac cgccccgaca cctgggtctc ctcgaccaag atgccgctcc
     2101 gcgacaacga ggggcagatc gtcggcacgt tcggcatctc gcgcgacatc accgagctga
     2161 agcgggccga gctggagctg gaaaaagccc gcaaggccgc cgaggacgcc agccgggcca
     2221 agagcgaatt cctggccaac atgagtcacg aaatccgcac gccgatgaac ggcatcatcg
     2281 gcatgaccga gctggctctc gacaccgagc tcacgcccga gcagcgcgaa tacctctcga
     2341 tggccaagag ctcggccgac tacctgctga cagtgatcaa cgacatcctc gacttctcga
     2401 agatcgaggc cggcaagctg gaaatcgaga acatcgactt cgccctgcgg gactgcgtcg
     2461 aagaaacggc cgccacgctc gccctgcggg cgcacaagaa gggcctggag ctggcctgcc
     2521 acgtccacca ggacgtgccc gacgccctga tcggcgatcc cggccgcctg cggcagattc
     2581 tcgtcaacct gatcggcaat gccgtcaagt tcacggagcg gggggaagtg gtcgtcgaag
     2641 tcaggagcca ggagccagtg accaggggcc agggatcgcc aacttctggt ccctggcccc
     2701 tggctcctgg ctcctacttc ttgcaattct ccgtccggga caccggcatc ggcatcccgc
     2761 cggaaaaggc cgggctcttg ttccaggcgt tctcgcaggt cgacagctcg acgacgcgga
     2821 agtacggcgg cacggggctg ggactggcga tctccgccca gctggcccag ctcatgggcg
     2881 gccgcgcctg ggtcgaaagc gaagtcggcc gcggcagcac gttccacttc accgcgcaat
     2941 tcggcatcgc gaccagccca ccgccggccc tgcctgtcga ggccgtgcgg ctcagcgacc
     3001 tgcccgtgct cgtcgtcgat gacaacgcga ccaaccgccg cattctgaag gagatgctca
     3061 ccaactgggg tctgcggccg acggtcgttc ccggcgctcg cgaagccctg gcggccctcg
     3121 acgcggcgcg ctgcgccggc cgggcgttcc cgctgatttt gctggacggc atgatgcccg
     3181 aaatggacgg cttcgagctc gccgcccgca tcaaacagca gccggacctc gtcggcgcgt
     3241 cgctgatgat gctctcgtcg gctgatcgcc gcgaggatgc ggcccgctgc aagcagctcg
     3301 gggtcgcgac gtatctcgtc aagccggtgc gccaatccga cctgctcgat gcgatcgtca
     3361 atacgctggc cgccaacgcc tcccaatccg ctccagttga cgccgaaccg ccgcctgcca
     3421 aacagcccgc cgtccttcag gccgagcggc ggctgaacct gctcctggcg gaggataacg
     3481 ccgtcaacca gcgcgtggcc gtgcggctgc tcgaaaagcg gggccacgag gtgaccgtgg
     3541 tgggcaccgg ccgccaggcg gtcgccgccg cgctggcgca gccgttcgac gcgatcctga
     3601 tggatgtgca aatgcccgag ctggatggct tcgaggcgac cgcggcgatc cgcgcggccg
     3661 agcacatgca cggcggccga cgcaagatca tcgccatgac cgcgcacgcc atgaaggggg
     3721 atcgcgagcg gtgccttcag gccggcatgg acggctacgt cagcaagcca ctccagcccg
     3781 aagtgctctt tgcggcgatc gagcggcccg cggccggcgg cgaaagccgg gagggaccga
     3841 cgccgctcga tgaggcagca atctccaagc actttggcga cgaggagttc ttccgggaag
     3901 tcgcgaaggt ctttctggaa agctgccccg actggctggc cgatctccgc gccgccgtga
     3961 cggcccgcga cgccaagcgg gtcctggtcg ccgcccacac gctcaagggc gccgtcagcc
     4021 acttcggggc ccaggccgtc tacgacgcgg cccatcaact cgagcaaatg ggcaagacgc
     4081 agcagctcga tgacgcgccg ccggcgctcg ccgccttggg ccgcgccctc gatgggctgc
     4141 aagccgccct ccgcgagcgc tccaaggtat gatcgagtca tgccttcgag cctgctggat
     4201 ctcccgcgct cgtccctcgc caggctgctg gccgactggg gcttctccgc agcgcatggc
     4261 gaccggctct ggcggtatct gtatcgcgag gcgaaggcgg atttcgccgc gatggaagag
     4321 ttgcctcggc ggctgcgcga gcggctgctg gccagcgcga acatcgacgc accgtccgtc
     4381 cttcgggaga ccgtggccga cggcggactg acgactaagc acttgctgga gctggccgat
     4441 gggcagcgga tcgagacggt gctcatgcgg ccatccggcc gcgtgaccgc ctgtctcagc
     4501 tcgcaggccg gctgcccgct gggctgcgtc ttctgcgcga ccggccagat gggtttcggc
     4561 cgcaacctgt cggccggcga gatcgtcgcg caggccgtgc acgtccagcg ccggctgggg
     4621 ccggccacca ggctgcggaa tgtcgtgctg atgggcatgg gtgaaccgct gctcaactac
     4681 gaatcggtac tgcgggccgc cgacatcctc ggcgacggcg gcgggctggc catcgccggc
     4741 aagcggctga cgctcagcac cgtgggcgtg gtgccgggca tcgtgcggct ggccgacgaa
     4801 gggcggacct gctcgctggc cgtctcgctg cacgcggcca cgcaggagga gcgggccgca
     4861 ctcgttcccg cggcggcgac ctggccgctg gcggacctga tgcgggcctg ccgctattac
     4921 gcggcgaagc tcggccgccg catcttcttc gagtggacgc tcatcgccgg acgcagcgac
     4981 tcgcccgagc aggctctcga gctggcccgg ctcgtgcgcg gcttgccggc ccacatcaac
     5041 ctgattccgc tcaatcccac ggccggctac gccgcagcgc cggccgaacc ggaagcggtg
     5101 cgccgttttc aagccgcgct tcgcgagcag ggcctgcctt gcaccatccg ccgccgccgc
     5161 ggcatcgcca tcgccgccgg ctgcgggcag ctggtcgttg ccgacgctgc cagcgccctc
     5221 gctcccgcct agtaccaatt cagcgatcgc tggaccagct cggccaccgt cggcggcggc
     5281 ggcggcgcgg ggttcgcgcc gtccaggtac tgccgccagg tgttcaggtc gttgcggaaa
     5341 ttgtcgcggc cggtgatcgc ggccagcgac tgcatggcgg cggtttgcag cgcggggtcg
     5401 cggtcgtcga gcgcgggacg cagcgactgc gccaggcgct tgtactcctc cgcgggcagg
     5461 ccggccgcgg cgaacttgcc cagcgcgtcg gcggccgcga tccgcacgtc gaggtcggcg
     5521 tccgcagaga ccgcgtgggc cagcggctcg accgcttccg gcgcgggatg ccgcgccagc
     5581 gcctggcagg cggcgatccg cacgttcggc gcttcatccg tcaaagcggc ttgcagcgcc
     5641 gagcgggccg ccggcgtggg cagctcgccc agcgtgcggg ccagctcggc ccgcagcacc
     5701 ggcgctgtct cggcttgcaa tccggcggcg agctgcgccg cgagcggttc ggcctgctcc
     5761 gccggcagtc gcgcggcctg cgcccgcagc gcggccaggt ccgcgacttt ccggtgatac
     5821 gtcgcgatcg cccgctcgtc cgcgtcccac tgccggcgaa cccacggatt gagagtgcgg
     5881 agctcgggca ccggaccatc cgcgcagccg gcagcgacca gcggcaggca ggccagtgaa
     5941 gcggccagga cgcgatgcat ggttacttga cccacggggg gcgggatata gcagactctg
     6001 gcgagcgggg ccagacgatt cgccctggac aaacggcccg ccgccgccac aatcggccgc
     6061 gaaggagccc gcgcttgacc gaatcgccgc caccctctgc tagcgaaccg ctgctgccgg
     6121 ccgccggctg gaccgcgcgg cgactcgtgc tggcagccgc ggtgctcatg gtattcgccg
     6181 cggcggccct gcccttggac ctggccgcgg cccgcttcgt gcaggcccat cgcctgacgg
     6241 gcgatctgcg gcgaatcgtg acgctggccg aagtcttcgg ctggggcggg acgatcgccc
     6301 tgatcatcct gacggccgtc gtgctcgatc cgcgcggctg gcgggtcgcc ccgcggctgg
     6361 cgatcggctc gctggccgcc ggcctggccg ccgatggcct caagctgctg ggagtggcgc
     6421 gctggcggcc ggccccggcg ctcgaggccg gactgaacca gacttttgtg ggttggctgc
     6481 cgctctggcg tcacgatccg gcgctgggag cttacgactc ccgcctccaa tcgttcccgt
     6541 cgggccacgc ggccaatgcc gtgggcctgg ccatcgccct ggccgtgctc tacccacgcg
     6601 gccgctggct gttcgccgca tttgcggtgc tcgcctgctt gcagcgagtc gagggcctgg
     6661 cccactttcc cagcgatgtt ctcgtcggcg cagccatcgg ctgtctggcc agcgcgatct
     6721 gcgccggacc gctgcaccgg cggctgacgg cgctcgaaca gtaggtcccg cctgccgggc
     6781 gggacctggg cggcgcgggc aagtccaaac tctgcacgtt ccaggtcgct gcgcttcccc
     6841 aaggcccctt ccggcagaag gagcctactt cttcggcggc ggcgcgagcg tcgagcgata
     6901 aacccagagg tggtcgatcg gcttgtcgat tccatactcc gccaggactt tcgcctgctc
     6961 ctccggtgtg ccgtcggtgc ggcagccgta gcggatttct tcctcgttgg gatcgacggt
     7021 gtcgccgggc cgccagaaat tcagctgcaa gctcttcttg gtgatctgcc ggccggtgcc
     7081 gggcggatcg ccgctcttat aggccccggc gggatcttcg aatcggtagg cgttggtcag
     7141 cccctggata tagattgcga aataatcgat ccgcagatcg acgtcggtcc aggtcaccac
     7201 gccccacacg ccgttgtctt ccttctcggt gctgaccttg agcgggacct cgctgatcga
     7261 cagcgagtcg tagagcagct ggtcggtgcg gccgggaaat tcgcgggcct tgatcggcgc
     7321 gagggcggcg ggaatcacgc ggtcgagata ctccttgtcg tactccagcg aggccagcac
     7381 gaaatgcggg aagaaccgca ggtcggtcgt cttgcggccg aacagctcga cgtcgttcgt
     7441 cgtcccgcgc ttgaaattga cgtgcacgcg cccgacctcg ccgctctcca gcttgacctc
     7501 ttcccgctcc tcgatgggcg tgatgtggct gcccaaattc cgcacccggt agaccatgta
     7561 ccacaccagc tgccgctgca tgcggttgct cgcctgcggt acatcgacgt ggaccatccg
     7621 catcggcttg aacgagaact ccaggttcca gatcgtccgc cgcagcgtcg cctctttgga
     7681 ccgctcgaag atcgtcgagg tcttgggcgc gagcttgggg tcgtattcca gcccgggaat
     7741 cgacacgggc acttccacca gcggacgcgg cccgctgaac atctcctcgg tttcgggctt
     7801 cggcggaatc gtggtgacca cgcccggcgc gaactgccgc ggcggcacgg tccgctgagc
     7861 ggcggccggc ccaatcgcgg aatgcaggcc cgccaaggcc accaggcagg caagagcggc
     7921 gcgtcggctc atagacgaat cttcttgtcg gggcgagagt tgcgatcgcc aggcgaggag
     7981 aatcccaaaa aaccagtcta tttgctggac tttggcattt caaccaaagc ccaaccctca
     8041 aaacaacaca gccaccaacc aattcgcgac cgggccggga tattcttccc ccggtcgcta
     8101 cgtttatgtc gtttaacccg gctccgggtt aaacgaccaa tcctccagca gctgaacctg
     8161 ccagttctcg gccctctggc cagtcgcggc caagaccagg cagccgcgaa ggcgcgtccc
     8221 ttccagccga aatcgctgct ggtttggggc gtttggggcg ctgggtaagg gctcatacgt
     8281 tccccagtcc actcgcgaca cgcatccgcg gtcgccgctc accgggcctt catagttcaa
     8341 atagtccagc cggtgatcgg gcagccgctc ggcagccacc ggctctccgc ccacggcggg
     8401 cagcgacagg gcggcccagg tccgcaggac gccgccaacc tcgagcatca ggtcaaaatg
     8461 ggccggccgc gcatagccgg gcggcgtttc gtgccgcaat acgacgaaac ggggcatggc
     8521 gtcattttaa ctggtctgtt tcagggcgat ttggcgaccg cgcccgccag ctccggcgaa
     8581 attcggagtt cggccaccgc ctggccgctc gcagcaggcc gcgctggtgg cgctttgcct
     8641 ttcctcgcgg cggggcggcc gttcatgcgc gcatggcgtg cggttttttc ggggcggccg
     8701 tttttggcgt aagtggttgt gcggtcggcg gttgcggcgt ggttttcggg cgcatcgtta
     8761 tgcgctttat gcgcgctggg ggcggaatcg gatgcggcgg gcgagttggt ggacacttgg
     8821 tacgatggta cttctgtctc ttcggcagcc gcggcgctgg cggactcctg ggcgagtagc
     8881 tcttcggcca gcgcctggcg ctcggcctct tcgcgctcct tcagccgggc ggcggcggcg
     8941 aactgctctt cccgctggcg gtcctgctcg tcgagttcgg cctggcgctg gcgggcccgc
     9001 tcgcgctgct cgtcgtaata gctcagcccg ttgcttttgg ccgattggcg ctcggcgcgg
     9061 agctgctcgg cggcggcctc ttcggcggcg agctgctcgg gcgaaagcgg gtccagcggc
     9121 ggcagctcgt cctgctggac cagcttgagg ttgtccatgt tgatccgata cgccagccgc
     9181 aggaaccggg cggcgaggcc gtggcggtcc tgggccgaaa acgtggtgcg gacttcgcga
     9241 tcggcgccgg gctgcgaatg cgggcgctgc agcgttcttg tggtcacatc gaccgtgctt
     9301 tccatctcgc cggcgatgcg gaggcagctg gccaggatca gctcgttgcg ctcgtaggtc
     9361 agccaccgct gggcccgcag ccgctcggcg tggct
//
LOCUS       DASRJB010000448         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_687080, whole genome shotgun sequence.
ACCESSION   DASRJB010000448 DASRJB010000000
VERSION     DASRJB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687080"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2520
                     /locus_tag="VFB80_11825"
     CDS             <1..2520
                     /locus_tag="VFB80_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN34505.1"
                     /translation="KKPDGFVRRFGGTCIAVNAQRAVGANVIDVIAGLKKATAALNEN
                     LLYPQGLELVQVYDESDYIHSAVGLVSDNLLWGSLFTFVTLLVFLRSGWSTVIIFMHI
                     LISTIGAFLVMALLGRSLNVPALGGLAFAVGMLVDNAIVMLENIFRRFQQGEDPETAS
                     VKGAGEVWSALLNATMANLAVFIPVLFIREEAGQLFRDIAIAISAAVALSMLVAVAVV
                     PTAAMRILQRVQRRTGSHEQNGSSRTGSRNRSALQSLAHAGWAPIAAILGVCDAVALW
                     MVERLLSVNRFLQRTILARSIFVLSVFVASCVATWLLLPKVEYLPGGNRNLVIAILLP
                     PPGYNLETLLKMGNEVEDGLKPYWDVDPNDPKVAQLDAPPIEDFFFVARSRQVFVGVR
                     SAHPTRAAELVPVIQRLGDRLPGTLLIAKQTSLFEQGLTAGRTVDIEITGPDIHKLVA
                     LGGRIFGQLKMGGGGQPPLMPEAQVFPQPSLDLSNPEIWITPKWVQAADMQLTAADLG
                     YAVDALVDGAYATDYYSGGDKIDLRIIGEKQFAKDSQSVAALPIATPTGQLVPLEAVA
                     NIQIASGPEQINHRTRQRAITLQVSPPAAMPLEAAMDLIRDQVITPLQASGELKGGYG
                     IALSGTAEKLTSTWNSLWLNLVLAVLITYLLMAATFESWLYPFVVIVTVPIGAVGGFL
                     GLWLLNWWVLQPLDVLTMLGFIMLVGTVVNNPILIVEQSLVHFREDGMPIGQAVIESV
                     RTRIRPIFMTTLGGLVGLLPLVLAPGAGSELYRGIGAVLLGGLLVSTVVTLVFVPALL
                     SLMMEAGNGLAHWLWPTPAAEPSLIIETPRPFTVPLEKVER"
BASE COUNT          466 a          886 c          870 g          529 t
ORIGIN      
        1 aaaaagcccg acggcttcgt tcgccgcttt ggcggcacct gcattgccgt caatgcccag
       61 cgggcggtcg gggcgaacgt gatcgacgtc atcgccggcc tgaaaaaggc gaccgcggcc
      121 ctcaatgaaa acctgctcta tccacagggg ttggagctgg tgcaggtcta tgacgaaagc
      181 gattacatcc actcagccgt cggcctggtg agcgacaacc tgctgtgggg cagcctgttc
      241 acgttcgtca cgctgctcgt gttcctccgc agcggctggt ccaccgtcat catcttcatg
      301 cacatcctga tcagcacgat cggggcattc ctggtgatgg cgctgctggg ccgttcgctc
      361 aacgtgccgg cgctcggcgg attggcgttc gccgtgggca tgctcgtcga taacgcgatc
      421 gtgatgctcg agaacatctt ccgccgcttt caacaggggg aagatccgga aacggcctcg
      481 gtcaaaggcg caggcgaagt ctggagcgcc ttgctcaacg caacgatggc caacctggcc
      541 gtgttcattc ccgtgctctt catccgcgag gaggcggggc agttgttccg cgacatcgcc
      601 atcgcgatca gcgccgcggt ggccctctcg atgctcgtgg ccgtcgccgt cgtgccgacc
      661 gccgcgatgc gaatcctgca gcgcgtgcag cgccgcactg ggtctcacga gcaaaacggc
      721 tcgtcgcgga ctggcagccg caatcgttcg gcattgcaat cgctcgcgca tgcaggctgg
      781 gctccgatcg ccgcgatcct tggcgtatgc gacgcggtcg ccctatggat ggtggagcga
      841 ttgctctcgg tcaatcgctt cctccagcgg acgatcctgg cccgcagcat ctttgtgctt
      901 tcggtctttg tcgcctcctg cgtggcgacg tggctcctct tgccgaaggt cgagtacctg
      961 ccgggcggca accgcaacct ggtcatcgcg atcctgctgc cgccgcccgg ctacaacctc
     1021 gaaacgctgc tgaaaatggg caacgaggtc gaggacggcc tgaagcctta ctgggatgtc
     1081 gatccgaacg acccgaaagt ggcgcagctt gacgccccgc caatcgagga tttcttcttc
     1141 gtcgcccgca gccggcaggt ttttgtgggc gtccgctcgg ctcatccgac gcgagccgcc
     1201 gagctggttc cagtcatcca gcggctgggc gaccggctgc caggaacgct gctcatcgcc
     1261 aaacaaacga gcctgttcga gcaggggctg actgcggggc ggacggtcga tatcgaaatc
     1321 accggcccgg acattcacaa gctcgtggcc ctcggcgggc ggattttcgg ccagttgaag
     1381 atgggcggcg gcggccagcc gccgctgatg cctgaagccc aggtcttccc gcagccgagt
     1441 cttgacctgt cgaatccgga aatatggatc actcccaagt gggtgcaggc ggccgacatg
     1501 cagctcaccg cggccgacct gggttacgcg gtcgatgcgc tcgtcgatgg ggcgtatgcg
     1561 accgactact actccggcgg cgacaagatc gatctgcgga tcatcggcga gaagcaattc
     1621 gccaaggatt cgcagagcgt ggcggccctg ccgatcgcta cgccgaccgg ccagctcgtt
     1681 ccgctcgagg ccgtggccaa cattcagatc gccagcggcc ccgagcaaat caaccaccgc
     1741 acgcggcagc gggcaatcac gctccaggtt tcgccgccgg ctgccatgcc gctcgaagcc
     1801 gcgatggacc tgatcaggga ccaggtcatc acgccgctgc aagccagcgg tgagctcaag
     1861 ggcggctacg gcatcgccct ttccggcacc gctgagaagc tcacctcgac gtggaactcg
     1921 ctgtggctaa acctcgtcct ggcggtgttg atcacctacc tgctgatggc tgcgacgttc
     1981 gaatcgtggc tctatccgtt cgtggtcatc gtcaccgtgc cgatcggcgc ggtcggcggc
     2041 ttcctgggat tgtggctgct caactggtgg gtgctgcagc cgctggacgt gctgacgatg
     2101 ctcggcttca tcatgctcgt gggcaccgtc gtgaataacc cgatcctcat cgtcgagcag
     2161 tcgctggtgc acttccgcga ggacggcatg ccgatcggcc aggcggtgat cgaaagcgtt
     2221 cgcactcgca tccgcccgat cttcatgacc acgctcggcg gattggtggg tctcctgccg
     2281 ctggtgctcg cgccaggcgc gggcagcgag ctttatcgcg gcatcggggc cgtgctgctc
     2341 ggcggcctgc tggtctcaac cgtcgtgacg ctggtgttcg tccccgccct gctcagcctg
     2401 atgatggaag ccggcaacgg gttggcgcat tggctctggc ccacgccggc cgccgaacct
     2461 agcctgatca tcgagacgcc gcggcccttc acggtgccac tggaaaaagt cgaacggtaa
     2521 gtattcgcgc ttcgcggcgc cgcgcgtctg gacagcggac cgcaactggc ctaaaaatac
     2581 ctgttcgaac aataatcgct cttccgttgg aggatttcct gtggccacgt tgaagattcc
     2641 agtcctgcgt tggggcaagc cgtatgaatc gctggaagtc gatgaggtca agcatttcat
     2701 caccggcgag ccaatcgcca aagtgagcca ggccaatggc gggatcattg g
//
LOCUS       DASRJB010000449         6957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3506937, whole genome shotgun sequence.
ACCESSION   DASRJB010000449 DASRJB010000000
VERSION     DASRJB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3506937"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFB80_11830"
     CDS             <1..265
                     /locus_tag="VFB80_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN34506.1"
                     /translation="HVMHHDELAFELDGMVKFVGLEDADEFLAQTDDLRRGYLQAVRQ
                     FSEKLQEIVHRNGCERVVCDTSRGLADQLLDYLNKRGMLVRMR"
     gene            511..1689
                     /locus_tag="VFB80_11835"
     CDS             511..1689
                     /locus_tag="VFB80_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN34507.1"
                     /translation="MRYSFRLAEVVGHVPDPRRRPGTIKAICDYTGLDRHQVAALLKN
                     EVKYIPLDALSKLCAYLIERGFAKADELPGRLFGVEPEHFWELLARRRRLELCLGVRR
                     SDADDTLDDAWVVASDSVLLGELLNGATTMGGTAGHTTGGPPAENDSDVQAANGLRPH
                     PAYLHQSLVWSPGQASLAECQAMAKSIYETFCEAAGDKALVCLGSNKSNPVVETVVAS
                     AFGCEPFVSQDGLPASARRSCPFFLRYRDNDPQFASCFGGLKVAKNQASQKPGIYYEQ
                     PDGGWGACEWEANKLDAAFVFYVHRESQGRMEMALGGFSGRATRMLARLLARRGDEFW
                     PPVYSDEGLQIGAFVVKFNLAGQLEDGLGRLEHTADDQIVRLDHEVIAKRMQHMAEAQ
                     "
     gene            1709..2458
                     /locus_tag="VFB80_11840"
     CDS             1709..2458
                     /locus_tag="VFB80_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34508.1"
                     /translation="MDLQGKAALVTGGTMGIGAAIAIELACRGADVAIAARNLGEPAH
                     QVRAATLKLGRRCLLLAADFTSPEDCTMAVEKAVQQFGRLDVLVHNAGGPSPGRIEEI
                     TPEQWRQTMDLHVNANFYLVRAALPHLKKHKEGAIVTISSTAGIRGVPGALAYATAKG
                     AIPQFTRSLARDLADDNIRVNCVAPGVIRTRFHADMTAERKELNLKHRIPLHREGTSE
                     QVAEVVALLVTNDYITGETIVIDGGLTSRIA"
     gene            2488..6066
                     /gene="dnaE"
                     /locus_tag="VFB80_11845"
     CDS             2488..6066
                     /gene="dnaE"
                     /locus_tag="VFB80_11845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZN34509.1"
                     /translation="MADARPFVHLHCHSHYSLLDGASEIGTLVKRAKELGMNALALTD
                     HGNLHGALDFYRKAKDEGINPIIGYEAYIAPSSRFEKKDAANSKEASYHLTLLAQNRT
                     GFRNLIKLASAASLEGFYYKPRIDKELLQAHHEGLICLSGCVSSELSLALIKGGAGEE
                     DFREAREIAGWFRQVFGERYFLEIMNNNVEVQRLQLSAAVDLGKRLGIPLVATSDAHY
                     VQQDDAVAQDVLLCINTGKFRTDTNRMRMENDSFYLRSPDEMYRHFPGLEDAVARSQE
                     IADGVGIDLDLGKRHFPVFQLPPKKTAEDYLRELCIRGLKERYAGDEELLPGGELSGV
                     VRERLERELSVINQLGFPNYFLIVWDFVRHAREQQVPATARGSGVGALVCYALYLSHV
                     CPIRYDLLFERFLDLNRKDAPDIDIDFCKDRRGEIIRYVKEKYGEENVAQIGTFGTLA
                     AKAAITDVGRVLGWPVPRVNALKALVPEQLGISLDEALQSSDELKRVYDSDAEAREVL
                     DLARKIEGLARNVGTHAAAVVIADKPLTEYVPLCRVKDKADIITQWSMGDVEAAGLLK
                     MDFLGLRNLTILTRAVDLIEQTTGQRVDPYKFPLDDKRTFALLQRGETKGIFQLESGG
                     IRDLLQKMKPDHFSDIIATNALYRPGPLEGGMVQEYVDVKHGRKSAEYVHPVCEKILS
                     ETNGVMVYQEQVMRILNELGGIDLAAAYTCIKAISKKKEETIAKNREQFVSGAKANGL
                     TEQQARDFWNLIIKFAGYGFNKSHSTAYALIAYMTAYLKAHYPVEFMAALLSGDIPGR
                     NFKTKDSLVEHLEDCQRMGIEVEPPDVNRSGVDFTVAGGKIQFGLSAIKGCGGSAGDA
                     IVAARQGGAFRDLFDFCERVDSTGASRATIETLIKAGAFDRLGGKRSQLTAAIDRAVQ
                     AGVSLAADRKSGQKSLFGGDDDAAAKTSVKLPDLPEWPDRERAQKEKEVLGFYLTSHP
                     LDEHKHTLAKYCSHSTADIPQLKDREEVILGGLLSVLKFSTTRDPKPGKPSKYVMFDL
                     EDVAGSIRTIVWPEQFAEMGHLVAPDAILLVRGVIDRRGGEEANLVVNELITLDQLDS
                     RYTTGIVVRIDEREHGESALDQIKEIVRAYPGSSELQLAVVLEDGSRVYLKSHRVRID
                     VTRELQDRIDSLLGPGNLQLLTSRPKPSSNGNGSRWSGASGRSAGR"
     gene            complement(6169..>6957)
                     /locus_tag="VFB80_11850"
     CDS             complement(6169..>6957)
                     /locus_tag="VFB80_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34510.1"
                     /translation="KVELMDLATNFQQQIKVPDAAAAKTTLIALAQRLKELPAPGTTP
                     ATSETPPEAPPAPGGDLARQWNEAHATAEPRYLAALKKGESKLTSELKVIFAYATERA
                     EAGEYDKALVAIKRLLPLLEQAERGPGPQVSSRLVESRKFLLTRFQKIRADLKQELDS
                     LQKAIAESAADDDPTELTTAMQGWFDRLLDELQNGIDASINAGDDFQQAQEVIHGLQA
                     EVDDDELIEHLLENPLIDGEQFVSAINGGLDDIATELRASVEVR"
BASE COUNT         1294 a         2168 c         2281 g         1214 t
ORIGIN      
        1 ccacgtcatg caccacgacg agctagcctt tgagctcgac ggcatggtca agttcgtggg
       61 cctggaggac gccgacgaat ttctcgccca gaccgacgac ctccggcgcg gctatctgca
      121 ggccgtgcgg cagttctccg aaaagctgca ggaaatcgtc caccgcaacg gctgcgagcg
      181 ggtcgtctgc gataccagcc gcggcctggc cgaccagctg ctggactatc tcaacaagcg
      241 cgggatgctg gtgcggatgc ggtaacggtg gttgatcggc ggtcggccag tcggtcggtc
      301 gcggacaccg atctaccggc ttaccgacca ccgactcacc gacccacacg gttgcccatt
      361 cccacgcaag ctgctagaac gcttgcaccg gttcatttcc cgttaagggc ctgttttggc
      421 cgtttagcgg gaattgaagc ggtccaaagc gccgtgatag cagtcggcct aaggcgcttt
      481 catgttccgt tagtgggcgg gaggcgggcc atgcgatatt cgtttcggct agcggaggtc
      541 gtggggcacg tacccgatcc gcggcggcgg ccgggcacga tcaaggcgat ctgcgactac
      601 accgggctgg accggcacca ggtcgccgcc ctgctgaaga acgaagtcaa gtacatcccg
      661 ctcgacgcgc tctcgaagct ctgcgcttac ctcatcgagc ggggtttcgc gaaggccgat
      721 gaattgccgg ggcgcttgtt cggcgtcgag cccgagcact tctgggaact gctcgcccgg
      781 cggcggcggc tggagctctg cctgggcgtg cggcggagcg atgccgacga cacgctcgac
      841 gacgcctggg tcgtggcttc ggactcggta ctgctgggcg agctcttgaa cggcgcgacg
      901 acgatgggcg gcaccgccgg ccacacgacg ggcgggccgc cggctgagaa cgattccgac
      961 gtccaggccg cgaacggtct gcggccgcac ccggcctatt tgcatcagtc gctcgtctgg
     1021 agcccggggc aggcttcgct ggccgagtgt caggcgatgg ccaagagcat ctatgagacg
     1081 ttctgcgagg ccgctggcga taaggcgctg gtctgcctcg gcagcaacaa gagcaacccg
     1141 gtcgtcgaaa cggtggtcgc ctcggccttc ggctgcgagc cgttcgtatc gcaggacggc
     1201 ctgccggcct cggcccgccg cagctgcccg ttcttcctgc ggtaccggga caacgacccg
     1261 caattcgcct cgtgcttcgg cgggctgaag gtggccaaga accaggccag ccaaaagcca
     1321 gggatttatt acgaacagcc cgacggcggc tggggggcgt gtgaatggga ggccaacaag
     1381 ctggatgcag cgttcgtgtt ttacgtgcac cgcgagtcgc aggggcggat ggaaatggct
     1441 ctgggcggct tttcggggcg ggccacgcgg atgctcgcgc ggctcttggc tcgccgcggc
     1501 gacgagttct ggccgccggt ctattccgat gaggggctgc aaatcggcgc attcgtcgtg
     1561 aagttcaacc tcgccggaca actcgaagac ggcctgggcc ggctggagca cacggccgac
     1621 gaccagatcg tgcggctgga ccacgaggtg attgccaagc ggatgcagca catggccgag
     1681 gcccaatagt tcgattcaga ggctggacat ggatctgcaa ggcaaagcgg cgctcgtaac
     1741 gggcggcacg atggggatcg gggcggcgat cgccattgag ctggcctgcc gcggtgcgga
     1801 cgttgcgatt gcggcacgta atctgggcga gccggctcac caggttcgcg ccgccaccct
     1861 gaaactgggc cggcgttgcc tgctcctggc ggccgatttc accagcccgg aagattgcac
     1921 gatggccgtc gaaaaagcgg tgcagcaatt cggccgcctc gacgtgctcg tgcacaacgc
     1981 cggcggccct tcgccgggcc gtatcgagga aatcacgccg gagcagtggc ggcagacgat
     2041 ggatctgcac gtcaatgcga acttctacct ggtcagggcc gcactgccgc acctcaagaa
     2101 acacaaggaa ggcgcgatcg tcaccatctc ctcgacggcg ggtattcgcg gcgtgcccgg
     2161 agcactggcc tatgccaccg ccaagggcgc catcccgcaa ttcacgcgga gcctggcccg
     2221 cgacctggcc gacgacaaca tccgcgtcaa ttgcgtagcc cccggcgtca tccgcacgcg
     2281 gtttcacgcc gacatgaccg ccgagcgcaa ggagttgaac ctgaagcacc gcattcccct
     2341 gcaccgcgag ggcacgagcg agcaagtcgc cgaggtggtt gcgctgctgg tcaccaatga
     2401 ctacattacg ggtgagacca tcgtcatcga cggcggcctc accagccgca ttgcgtagat
     2461 tggattctct tagcacggat cgctttcatg gccgacgctc gccccttcgt tcatttacac
     2521 tgccactcgc actacagcct gctcgacggg gccagcgaga tcggcacgct cgtgaagcgg
     2581 gccaaggagc tggggatgaa cgccctggcg ctaaccgacc acggcaacct gcatggcgcg
     2641 ctggatttct atagaaaggc caaggacgaa gggattaacc cgatcatcgg ttatgaggcc
     2701 tacatcgcgc ccagcagccg cttcgagaaa aaggacgccg ccaacagcaa ggaagcgagt
     2761 tatcacctga cgctgctggc tcagaaccgc acgggcttcc gcaatctgat caagctggcc
     2821 tcagccgcgt cgctggaggg tttttactac aagccgcgga tcgacaagga gctgctgcaa
     2881 gcgcaccacg aagggctgat ctgcctgtcg gggtgtgttt cgagcgagct gagcctggcg
     2941 ctgatcaagg gcggtgccgg cgaagaggac ttccgggaag cccgcgagat cgctggctgg
     3001 ttccggcagg tgttcgggga gcggtacttc ctggagatca tgaacaacaa cgtggaggtg
     3061 cagcgactgc agctctcggc ggcggtcgat ctggggaagc ggctcggcat tccgctggtg
     3121 gcaacgagcg acgcccacta cgtgcagcag gacgacgcgg tggcccagga cgtgctgctg
     3181 tgcatcaaca cgggcaagtt ccgcaccgac acgaatcgga tgcggatgga gaacgactcg
     3241 ttctatctcc gttcgccgga cgaaatgtac cggcattttc caggcctgga ggacgcggtc
     3301 gcccgcagcc aggaaattgc cgacggcgtc ggcatcgacc tggatctggg caaacggcat
     3361 tttccggtct ttcagttgcc gccgaagaag accgcggaag actacctgcg ggaattgtgc
     3421 atccggggcc tgaaagagcg ctatgccggc gacgaggagc tgctgcccgg cggcgagctg
     3481 tcgggcgtgg tgcgcgagcg gctggagcgc gagctgtcgg tcatcaacca gctgggattt
     3541 cccaactact tcctgatcgt ttgggatttc gtgaggcatg cccgcgaaca gcaggtgccg
     3601 gcgaccgccc gcggcagcgg cgttggggcg ctggtttgct acgcgctgta tctgagccac
     3661 gtctgcccaa tcaggtacga cctgctcttc gagcggttcc tcgatctcaa ccgcaaggac
     3721 gcgcccgaca tcgatatcga cttctgcaaa gaccgccgcg gcgagatcat tcgctacgtc
     3781 aaggagaagt acggcgagga aaacgtagcc cagatcggca cgttcggcac gctggcggcg
     3841 aaagccgcga tcaccgacgt cggccgcgtc ttgggctggc ccgtgccgcg ggtcaacgcg
     3901 ctcaaggcgc tcgtcccgga gcagctcggt atctcgctcg acgaagcgct ccagtcgagc
     3961 gacgaactga aaagggtcta tgactcggac gcggaagcgc gcgaagtgct ggacctggcg
     4021 cgcaagatcg aggggctggc ccgcaatgtc ggcacgcacg ccgccgcggt cgtgatcgcc
     4081 gacaagccac tcaccgagta tgtgcccctc tgccgcgtga aggataaggc cgacatcatt
     4141 acccagtggt cgatgggtga cgtcgaggcc gcgggactct tgaagatgga tttcctgggc
     4201 ctgcggaacc tcaccatcct gacgagggcc gtcgatctga tcgagcagac gaccggccag
     4261 cgggtcgatc cttataagtt tcccctcgac gacaagagga catttgccct gttacagcgc
     4321 ggcgaaacca aaggcatctt tcagctcgag agcggcggca tccgcgacct cctgcaaaag
     4381 atgaagcccg accacttcag cgacatcatc gccaccaacg cgctgtatcg ccctggcccg
     4441 ctggaaggcg gcatggtgca agagtacgtc gatgtcaagc acggccgcaa gtccgccgaa
     4501 tacgtccatc ccgtctgcga gaagattctc tccgagacca acggcgtgat ggtctatcaa
     4561 gagcaggtga tgcggattct caatgagttg ggtggaatcg acctggcggc cgcctacacc
     4621 tgcattaagg ccatcagcaa gaagaaagaa gaaacgattg ccaagaatcg cgagcagttc
     4681 gtcagcggcg ccaaggccaa cggcctcacg gagcaacagg cccgcgactt ctggaatctg
     4741 atcatcaagt ttgcggggta cggattcaac aagtctcaca gtacggcgta cgctctcatc
     4801 gcctatatga cggcgtattt gaaggcgcat tatcctgtcg agttcatggc cgcgcttctg
     4861 tcgggcgaca ttcccggccg gaatttcaag accaaggact cgctcgtcga acatctggag
     4921 gattgccagc ggatggggat cgaggtggag ccgccggacg tgaaccgctc gggtgtcgat
     4981 ttcacggtgg cgggcggcaa gatccagttc ggactatccg cgatcaaggg ctgcggaggc
     5041 agcgcgggcg acgctatcgt cgcggcccgc caaggcggcg cgttccgcga cctgttcgac
     5101 ttttgcgaac gcgtggacag cacaggcgct agccgggcca cgatcgaaac gctgatcaag
     5161 gcgggcgcgt tcgatcggct gggcgggaag cgctcgcaac tgacggctgc catcgaccgg
     5221 gccgtgcagg ccggggtttc gctggcagcc gaccgcaaga gcggccagaa gtcgctgttc
     5281 ggcggtgacg acgacgcagc cgcgaaaacc agcgtcaagc tgcccgatct gcccgaatgg
     5341 cccgaccgcg agcgggcaca gaaggagaag gaagtcctcg gcttttacct gaccagccat
     5401 ccgctcgacg agcataagca cacgctggcc aagtattgct cgcactcgac ggccgacatt
     5461 ccgcagctca aggatcgcga ggaggtgatc ctggggggcc tgctttcggt cctcaagttt
     5521 tccacgactc gcgacccgaa gcccggtaag ccgtcgaagt acgtaatgtt cgacctggaa
     5581 gacgtggccg ggtcgattcg cacgatcgtc tggcccgaac aatttgccga gatgggccac
     5641 ctcgtcgcgc ccgatgcgat cctgctggtt cgcggcgtga tcgaccgccg cggcggcgaa
     5701 gaggccaacc tggtcgtgaa cgaattgatc acgctcgacc agctcgattc acgatacacg
     5761 acgggcatcg tcgtgcgcat cgacgagcgc gagcacggcg aaagcgccct cgaccagatc
     5821 aaggagatcg tccgcgcgta tcccggctcc agcgagctcc agctggcggt cgtcctcgaa
     5881 gacggcagcc gcgtctatct caagtctcac cgcgtccgca tcgatgtcac gcgcgagctt
     5941 caggaccgca tcgacagcct cctcgggccc ggcaacctgc aactgctcac cagccggccc
     6001 aagccgagtt ccaacggcaa cggttcgcga tggagcggcg ctagcgggcg ctcggcaggc
     6061 aggtgaaagc cgcgcagcat gcgcaccacc ggaggttatt gcggccccga cttttcgtcg
     6121 gggtactcga gccgctgcgg cgatcccgcg tgttcgctgg cgaacgtctc aacggacctc
     6181 cacggaggcg cggagctcgg tggcgatgtc gtccagtcct ccgttgatgg cgctaacaaa
     6241 ctgctcgccg tcgatcaggg ggttttccag cagatgttca atcagctcgt cgtcgtcgac
     6301 ttcggcttgc agcccgtgaa tgacttcttg agcctgctga aagtcgtcgc ctgcattaat
     6361 cgaggcgtcg atgccattct gcaactcgtc gagcagacgg tcgaaccagc cctgcatcgc
     6421 ggttgtcagt tcggtgggat cgtcgtcggc ggcggattcg gcgattgcct tctggagcga
     6481 gtccagctcc tgcttgaggt cggcccgaat cttctggaat cgcgtcagga ggaactttcg
     6541 cgactcgacc agccgcgatg agacctgtgg gccagggccg cgctcagcct gctccagcag
     6601 gggcaacagc cgctttatcg ccacgagggc cttgtcgtac tcgccggctt cagcccgctc
     6661 agtggcgtag gcaaagatga ccttgagctc gctggtcaac ttgctctcgc ctttcttcag
     6721 ggccgccagg tagcgcggct cggcggtggc gtgggcctcg ttccactgtc tggccaggtc
     6781 gccgcctggg gcgggcggcg cttccggcgg agtctcggaa gttgctggtg tcgtgccggg
     6841 cgcgggcagc tccttcaacc gctgggccag ggcgatcagc gtcgtcttgg cggctgcggc
     6901 atcgggcact ttgatttgct gctggaaatt cgtcgccagg tccatcaact cgacttt
//
LOCUS       DASRJB010000450         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4073759, whole genome shotgun sequence.
ACCESSION   DASRJB010000450 DASRJB010000000
VERSION     DASRJB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4073759"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFB80_11855"
     CDS             <1..1010
                     /locus_tag="VFB80_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34511.1"
                     /translation="LALRLEGYEHAEIAERLGCHERTVRRDLAEARRVLTARAGGDFI
                     PTAARRAPPRSLQPRREVSQELLALALLPWSDFVLREQIGAGASGRVYRALRRSTGHE
                     VAVKFLRKSLRDNPAAVERFLREALTVSKLHHPGIVAVHGAGRTPGGGFFLLMDLVPG
                     RDLERVARGETVEPRRAGAWVAEAARAVHAAHERGVIHCDLKPGNLLLDAGGRIRVTD
                     FGLAMPLSSDRTDLLPAGGTPAFMAPEQADPRWGAISPRTDVYGLGAVLHFLLYSRPP
                     YTAASAAEVLSQVASPEPVPVPAFSIDPRLREILVKALCKPPAQRFATGAELAEALRG
                     P"
     gene            complement(1015..1662)
                     /locus_tag="VFB80_11860"
     CDS             complement(1015..1662)
                     /locus_tag="VFB80_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN34512.1"
                     /translation="MEDLLQQAAQASQRGEHQTAIARLSDAIQQSPMTALPWYLRGRE
                     HFRTGKIKESVADFDRYVQLAPRAESQQWERGIAYYYAGEYKKGAKQFADYQTFHDQD
                     VENSAWRYLCVAKDEGVEQARKNLLPIKSDTRVPMMSIYKLYQGENKPEEVLAAAGGD
                     NQALFYAHLYIGLWHEVNGRPAEAKSHILEAEKHKISHYMWDVAHVHAERLRAEK"
     gene            2208..2447
                     /locus_tag="VFB80_11865"
     CDS             2208..2447
                     /locus_tag="VFB80_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HZN34513.1"
                     /translation="MLVLSRKIGERLVIGDNITVVVSRVAGNRVTLGIEAPSEVRIIR
                     GELKATGGDGPHARAAVGYHQSADGLALSLPGLAK"
     gene            complement(2458..3627)
                     /locus_tag="VFB80_11870"
     CDS             complement(2458..3627)
                     /locus_tag="VFB80_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN34514.1"
                     /translation="MQNADGPNEALALDGGQPLVPEGPPPWPRPDEAVEAALAAAYAD
                     GSWGKYHGPHCERLRELLCQLHGVKQAWLCSSGTIAVELALRGLRIGVGDEVILAAYD
                     FPGNFRAIEAVGAAPVLVDLAAGTWTIEAEQVAAAIGPQTKAVIVSHLHGSLADMRRL
                     REIADQGGLAIVEDASQVPGATIAGRPAGSWGDVGVLSFGGSKLLTAGRGGALVTHRD
                     DVLQRVRIFCERGNDAFPLSELQAAVLAPQIPQLAAANRQRLSAVHRLLGACGAMPGL
                     IPLRLADSADLPAFYKLPWLVAGNSDECSSPHFEERRRWFIAAGRAEGLALDEGFRGF
                     VRRSASRCRTVGDLSHARCASAGTLLLHHPVLLTGGDTIDRAALALRRIAAAKQH"
     gene            complement(3647..4999)
                     /locus_tag="VFB80_11875"
     CDS             complement(3647..4999)
                     /locus_tag="VFB80_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN34515.1"
                     /translation="MPKPTDIQIRNVTVETERIDYRSPIKFGGRVVTDAVLLNVTVEV
                     ESAGGRRGSGFGSMPMGNVWAWPSSVVSVPQSLEAMLQFALAAAEAARDHLEFAHPLD
                     LTRQLAAGHPALAAAVVKKLNLAEPMPKLAELVAASPLEAAIHDAYGKTLQANSYNVL
                     GPEFVAHDLAHYLTPEFAGEYLDRYTLRHPKEKMPLYHLVGALDPLVDSDINQRLNDS
                     LPETLGEWILMDGLTHLKIKLNGDDLPWDVERVVGVERVAAQTQASAGVTSWNYSLDF
                     NEKCASVEYLLEFLRKLEERSPAALGRVHYIEQPTHRDLKKHPENKMHKAAAIKPVVI
                     DESLTDLESLQLAREQGYSGVALKACKGQTEALLMGAAAQKYDMFLCVQDLSCPGWSF
                     LHSASIAARLPTIVAIEGNSRQYCPAGNRTWGELYPSMFHITDGTVGTGVLTGNGLGF
                     "
BASE COUNT          820 a         1670 c         1672 g          883 t
ORIGIN      
        1 cgctggccct gcggctggaa ggctatgagc acgcggagat cgccgagcgg ctcggctgcc
       61 acgagcggac cgtgcggcgc gacctggccg aagcgaggcg cgtgctgacg gcccgcgccg
      121 gcggcgattt cataccaacg gcggcccgcc gcgccccgcc gcgcagcctc cagccgcgcc
      181 gcgaagtttc gcaggagctg ctggcactgg cgctgctgcc ctggagcgac ttcgtgctcc
      241 gcgagcagat cggcgcaggg gccagcggca gggtttatcg tgcactgcgg cgcagcaccg
      301 gtcacgaagt cgccgttaag ttcttgcgga aatcgctccg cgacaacccg gcggcagttg
      361 agcgtttcct gcgcgaggca cttacggtat ccaagctcca tcatccgggg atcgtggccg
      421 ttcacggcgc ggggcgcacg ccaggcggcg gcttcttcct gctgatggac ctcgtgccgg
      481 ggcgcgattt ggagagggtc gcccgcggcg aaacggtcga gccgcgacgg gccggagcct
      541 gggtcgcgga agcggcgcgg gccgttcacg cggcccacga gcggggcgtg atccactgcg
      601 atctcaagcc gggcaatctg ctgctggacg ccggcgggcg gattcgcgtc acggactttg
      661 gcctggcgat gccgctctcg tccgaccgca ccgatttgtt gccggcaggg ggcacgcccg
      721 cgttcatggc ccccgagcag gccgatcctc ggtggggcgc gatttcgccg cgcacggatg
      781 tgtatggcct gggcgcggtg ctgcatttcc tgctgtattc ccggccgccc tacacagcgg
      841 ccagcgcggc agaggttttg tcgcaggtcg ccagccccga accagtgccc gtgcccgcgt
      901 tctccatcga cccgcggctg cgagagattc tcgtgaaggc cttgtgcaag ccgcctgccc
      961 agcgatttgc caccggggcg gaattggccg aagcgctccg aggaccgtga gcggctattt
     1021 ctcggcccgc agccgctcgg cgtgaacgtg cgctacgtcc cacatgtagt gagagatctt
     1081 gtgtttttcg gcttccagga tatgcgactt cgcctcggcc ggacggccgt tgacctcgtg
     1141 ccagaggccg atatagaggt gggcgtagaa cagcgcctga ttgtcgccgc cggcggcggc
     1201 cagcacttcc tcgggcttgt tctcgccctg gtagagcttg tagatgctca tcatcgggac
     1261 gcgggtgtcg cttttaatcg gcagcagatt cttccgggcc tgttcgaccc cttcgtcttt
     1321 cgcgacgcac aagtaccgcc aggccgagtt ctcgacatcc tggtcgtgaa aggtctggta
     1381 atctgcgaac tgcttggcgc ccttcttgta ttcgccggcg tagtagtacg cgatgccgcg
     1441 ctcccactgc tgcgactcgg cccggggcgc gagctgcacg tagcggtcga aatcggccac
     1501 cgattccttg atcttgccgg tgcgaaagtg ctcccggccg cggagatacc agggcagcgc
     1561 cgtcatcggc gactgttgga tcgcgtccga cagccgcgcg atggcggttt gatgttcgcc
     1621 ccgctgcgag gcctgggcgg cctgctgaag caggtcctcc accgaatcag cgccggcaga
     1681 acaggagcaa gcgaggcaca ggagcaacgg aaaactgcaa cgtatcaaca tcggtacgat
     1741 gggtgtcaca agagggactg ccacgaacga tgccgttgac ggtacggcac agcgccaccg
     1801 gatgcaagtc gcggctgatg cgtgggcgtc aagctggtcg cttggctgcc agcatgtcct
     1861 ttgaacgcgc tcaatgtggg acgcgagtct cacttatgga cgcatgcaga taccgctagc
     1921 gaagggtgtt gagagctggc ggacccgcga ctgactttcg catggcgcaa aattccagga
     1981 tttcgaacaa aaatcggggg acactacaat tttcattcaa gaaacatgcg gtcggcccga
     2041 acgttgctgc attctggccg cacgaacgtt gactgccgac ttgacggcgg cgaatggtgc
     2101 cggggggcca tacttcgaaa cgctggaaaa gtgaaacagg cggatgattc gcaagctggc
     2161 cacccgcaat tcggccggcg accaaccttt caaggaggat cgtgctcatg cttgtgctca
     2221 gtcgaaaaat tggcgaacgg ctcgtcattg gcgacaacat taccgtggta gtcagccgag
     2281 tggcgggcaa tcgcgtgacg ctggggattg aagccccttc cgaggtccgc atcatccgcg
     2341 gcgagttgaa ggccacgggc ggcgacggtc cgcacgctcg cgccgccgtc ggctatcacc
     2401 aatcggccga tggcttggcc ctctcccttc cgggcctggc gaaatagtat tcagccgcta
     2461 atgctgcttc gccgcggcga ttctgcgcaa agcgagcgcc gcgcggtcga tcgtgtcgcc
     2521 gcccgtgagc agcacggggt gatgcagcag cagcgtcccc gcgctggcgc agcgcgcgtg
     2581 ggacaaatcg ccgaccgtgc ggcagcggct agcgcttcgc cgcacgaagc cgcgaaagcc
     2641 ctcgtcgagg gcgagcccct cggcccttcc agccgcgatg aaccagcggc ggcgctcttc
     2701 gaaatgaggc gagctgcatt cgtcgctgtt ccccgcgacg agccagggca acttgtagaa
     2761 agccggaagg tcggcagagt cggcgagccg caggggaatc agcccgggca tcgcgccgca
     2821 cgcgcccagc aggcggtgca cggcggaaag gcgctgccga ttggcggctg ccagctgcgg
     2881 aatctggggc gcgagcacgg cggcttgcag ctcgctcagc ggaaatgcgt cgttgccgcg
     2941 ctcgcagaag attcgcacgc gctggagcac gtcgtctcga tgggtgacga gcgccccacc
     3001 gcggccggct gtcagcagct tgctgccgcc gaagctgagc acgcccacat cgccccagga
     3061 gcccgccggc cggccggcga tcgtcgcacc gggaacctgg gaggcgtctt cgacgatcgc
     3121 cagcccgccc tggtcggcaa tctcgcgtag ccggcgcatg tcggccagcg agccgtgcag
     3181 atgcgagacg atgaccgcct tcgtttgcgg cccgatcgca gccgcgacct gctcggcctc
     3241 gatcgtccaa gttcccgccg cgagatcgac gagcacgggc gccgcgccga cggcctcgat
     3301 cgcgcggaag tttccgggaa agtcgtaggc ggcgaggatc acctcgtcgc cgacgccgat
     3361 cctgaggccg cgcagcgcga gctccacggc aatcgttccg gacgagcaga gccaggcttg
     3421 ctttacgccg tgcaactggc agagcagctc gcgcagccgc tcgcaatgcg ggccgtgata
     3481 cttgccccag ctgccatcgg cataagccgc tgccagcgcc gcctcgacgg cctcatccgg
     3541 ccgcggccac ggcggcggac cttctggcac gagcggctgg ccgccgtcga gtgctaacgc
     3601 ttcgttggga ccatctgcat tctgcattct gaattctgca ttcccatcag aacccaagtc
     3661 cattccccgt cagcacgcct gtccccaccg tcccgtccgt gatatggaac atgctcggat
     3721 acagctcacc ccaggtgcgg ttccccgcgg ggcaatactg ccggctgttg ccttcaatcg
     3781 ctacgatcgt gggcaagcgg gcggcgatgc tggccgagtg caggaacgac cagccggggc
     3841 agctcaggtc ctgcacgcag aggaacatgt catatttctg cgcggcagcg cccatgagca
     3901 gcgcctcggt ctgtcccttg cacgccttga gcgccacgcc gctgtagccc tgctcgcggg
     3961 cgagttgcag gctctccagg tccgtcagcg attcatcgat cacgacgggc ttgatcgccg
     4021 cggccttgtg catcttgttt tccgggtgct ttttcaggtc gcggtgcgtt ggctgctcga
     4081 tgtaatgcac gcggcccaag gccgccggcg agcgctcctc gagcttccgc aggaattcga
     4141 gcaggtactc gacgctggcg cacttctcgt tgaagtcgag cgagtagttc caggacgtga
     4201 cgccggccga ggcctgcgtc tgggccgcga cgcgttcaac gccgacaacg cgttcgacat
     4261 cccagggcag atcgtcgccg ttgagcttga tcttcaggtg cgtcagaccg tccatcagaa
     4321 tccactcgcc cagtgtctcc ggcaggctgt cgttcagccg ctggttgatg tcgctgtcga
     4381 ccagcggatc gagcgccccg acgaggtgat acagcggcat cttctccttg ggatggcgga
     4441 gcgtgtagcg gtcgaggtat tcgccggcga actcgggcgt gaggtaatgg gccaggtcgt
     4501 gtgcgacgaa ctccggcccc agcacgttgt aggaattggc ttgcagggtc ttgccgtagg
     4561 cgtcgtggat cgccgcttcc agcgggctcg ccgcgaccag ctccgccagc ttgggcatcg
     4621 gctccgcgag gttgagcttc ttcacgaccg ccgcggcgag cgccggatgc cccgcggcca
     4681 actggcgcgt caaatcgagc ggatgtgcga attccaggtg atcgcgggcc gcctccgccg
     4741 ctgccagggc aaactgcaac atcgcctcca gcgactgcgg cacggagacg acgctgctcg
     4801 gccaggccca cacgttgccc atcggcatcg agccgaaccc actgccccgc cgcccgcccg
     4861 ccgattcgac ttccacggtt acgttcagca gcaccgcatc cgtcacgacc cgcccgccaa
     4921 acttgatcgg cgagcggtag tcgatccgct ccgtttcgac cgtcacgttt ctgatctgaa
     4981 tatcggtcgg cttcggcatg cggcacccct ggcggcttcg cggctgcggc ctatcctagg
     5041 gcagg
//
LOCUS       DASRJB010000451         6761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2381834, whole genome shotgun sequence.
ACCESSION   DASRJB010000451 DASRJB010000000
VERSION     DASRJB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381834"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFB80_11880"
     CDS             <1..329
                     /locus_tag="VFB80_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZN34516.1"
                     /translation="DVDVHASDVIIYDAQGYEPLKKFLLSNGIRHVLLTGYATDMCFC
                     RTTAGYENLARDFNVFLVGDASLATFPANRSPRFAVNATISLAALNHLITQVSWVKLD
                     PPAAAR"
     gene            359..1348
                     /locus_tag="VFB80_11885"
     CDS             359..1348
                     /locus_tag="VFB80_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN34517.1"
                     /translation="MLLTRRSWMRNAAAATTAALVPAQLRAAESDKALVAITLDLEMS
                     RNFPTWETTHWDYEKGNLNDETKRYTTAAAKRVKAAGGVLHCFAVGRVFEQADVTWLK
                     EIVEAGHPVGNHTYDHINVTAARPEDLQFRFQRAPWLLRGQSVAEAIRENVVLTNLAM
                     KSRLGMEPAGFRTPGGFRDGLREHPAVRAILKDLGFKWISSLYPPHPNSAAMEEPSAE
                     VLDSIVAALPKAQPFAYSDDLIEVPMSPISDIGAFRTGRWRLEWFLRAIRLCVEWTIE
                     NRAVFDFLAHPSCLYVVDPEFRAIDLICDLVRKAGPRAALVDLGTIAKRAVGR"
     gene            complement(1398..2483)
                     /locus_tag="VFB80_11890"
     CDS             complement(1398..2483)
                     /locus_tag="VFB80_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZN34518.1"
                     /translation="MPAPQILPPKLQQCLAVAVAQGASDLHLAAGYQPAIRLHGDLVE
                     LAGPPLAEAELQGMFSQLCPGELFAKLLAQKNLDWAFDAELDGQLCRFRANLFHAGGC
                     LAACLRIVPPKIPSFEWAGFPRPLAEKLAFLRDGLVIFTGATGSGKTTSLAMIVNLLN
                     QAGGYRIITVEEPLEYQFPHAPHSLVTQREVGPDVLSFADGLKYGLRQDPDVILVGEV
                     RDRETAQMALSAAETGHLVFTTLHTRDAKGAISRYADLFPQDVQSDVRYQLASSLRAV
                     VAQRLLPALERGAKRHLALEVLWNITPIVAGIRQGKLESIDNYLLTSKGEGLISFDES
                     VRQLFRAGLITRQIAEQNVRDLGYLNR"
     gene            2588..3811
                     /locus_tag="VFB80_11895"
     CDS             2588..3811
                     /locus_tag="VFB80_11895"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HZN34519.1"
                     /translation="MTTVAPPLPSPPAPQPRLASIDAYRGLVMFLMMAEVLRLSAVAK
                     GLIEAGHPSDFWNFLAWHQTHVEWAGCTLHDLIQPSFSFLVGVALPFSIASRVAKGQS
                     RWQMLAHAVLRAVILVLLGVFLRSMDYKQTRWTFEDTLSQIGLGYVPLFLVGLTRQRW
                     QWLALAVILVGYWGAFALYEPPVNFDYQEVGVPADWQYHYSGLASHWNKNSNLAWGFD
                     TWFLNLFPREKKQPFTHNGGGYATLSFIPTLATMILGLIAGGWLRLDEAAWKKVGSFV
                     AVGGLLIALGLGAHESGLCPCVKRIWTPSWTLYSGGWCFLLLAFFYTFIDVTSWTSWS
                     FPLRVIGANSIAAYLLAHGPDQFILRSFRIHFGQQIFEAFGKEYVHVVAGVAVLIVQW
                     LILYWLYRQKIHIRI"
     gene            3828..4700
                     /locus_tag="VFB80_11900"
     CDS             3828..4700
                     /locus_tag="VFB80_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN34520.1"
                     /translation="MVRLLSPLALLACSLAVAAEPTRVLILVGPSTHPPGTHEVAAGG
                     RLIEHCLEHMENVSGIEAEVIDKWPDDRMRLQKVASVVFTGDRFPPAEMPDRERIMAD
                     LSAMMDHGCGLVCYHYATGLGASHVTPEGDHPLLRWMGGYFATRCPHHQSIARIFDSA
                     TIEPDGGDHPVLRGWRAFTIRDEPYIKNYFGKEGPAKNVTILATSMLPPEAPAREAVA
                     WAVSRPDGGRGVGIVMPHFYKNWQNEDLRRLILNGIVWSAKLEVPKEGVRTTLPDLAT
                     FKPAAVEPPPTKAK"
     gene            4783..5823
                     /locus_tag="VFB80_11905"
     CDS             4783..5823
                     /locus_tag="VFB80_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34521.1"
                     /translation="MPDELPRYDRTQTYRWNYDHAPDSPPAVDVPPVGGSWTVCRRPV
                     GSPLGISAGPLLNGRWILYYAALGFDLLTYKTTRSRFRECYDLPNLQPVETGPIHTAG
                     GTLAAMDEMRGDWAISFGMPSREPADWRADVEWTRRRLPKEKLLSVSVVATQQPGWTL
                     DDLADDFARCAKWALDSGADVAETNFSCPNVTTVDGQLYQQPAGAGLVAQRVRAAIGR
                     APYSIKIGLVESDDLAGELIAAIGPFADALAMTNTISAKVRRTDGAPLFGGQSRGIGG
                     ESIRAASLAQVARFARIIKQQRLPTRITGCGGISSAEHVRDYLAAGAESVQLATAVMV
                     DPLVGCKIRREL"
     gene            complement(5828..>6761)
                     /locus_tag="VFB80_11910"
     CDS             complement(5828..>6761)
                     /locus_tag="VFB80_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN34522.1"
                     /translation="EADALKCGALATPFGCDWDGDGDTDIVCGNTAGYIELFENLSGL
                     KVAEPKWAAPRRLEAGGKTFRVMAGENGSVQGPAEAKWGYTTLNVADWDADGLPDIVF
                     NSIWGKVQWLRNVGTRTAPRLAEPQPIEVEWAGPTPKPAWVWWHLQGKELVTQWRTTP
                     VVYDFTGDGLLDLAVLDQEGYLALFERAKLDGRLTPRAPRRAFVDEGGKPLWLNPRAA
                     GASGRRKLCVTDWDGDGRFDILLNSANADFVRQIGQRGGQWVFQNAGALAPTNIEGHD
                     VSPATVDFDGDGIPDFLGGAEDGRLYFLRNPRSK"
BASE COUNT         1101 a         2223 c         2200 g         1237 t
ORIGIN      
        1 ccgacgtgga cgtgcacgcc agtgacgtga ttatctacga cgcccagggt tacgagccgc
       61 tgaagaagtt tctgctaagc aacggcattc gccacgtgct gctcactggc tatgcgaccg
      121 acatgtgctt ttgccgcacg acagccggct acgaaaatct ggcccgcgac ttcaatgtgt
      181 tcctcgtggg cgacgcctcg ctggcgacct ttcccgccaa ccgctcgccg cggtttgccg
      241 tcaacgccac catctcgctc gcggcgctga atcacctgat cacgcaggtc tcctgggtca
      301 agctcgaccc gcccgccgcc gcgcggtgac actaacccga cgcgccagcg agggagtcat
      361 gctcctcacc cgtcgctctt ggatgcggaa tgccgcagcg gccaccaccg cggcgcttgt
      421 gcccgcgcag ctgcgagcgg ccgaatcgga taaggccctg gtcgcgatta cgctcgacct
      481 ggagatgagc cgcaactttc cgacctggga gacgacgcac tgggactacg agaagggcaa
      541 tcttaacgac gagacgaagc ggtacacgac cgccgccgcg aagcgggtga aggcggccgg
      601 cggcgtgctg cactgtttcg cggtcggccg agtcttcgag caggccgatg tcacctggct
      661 caaggaaatc gtcgaggccg gccatccggt gggcaaccac acctacgacc atatcaacgt
      721 cactgccgca cggcccgaag atttgcagtt ccgtttccag cgcgctccgt ggctgttgcg
      781 cgggcagtcg gtcgctgagg cgatccgcga gaacgtcgtg ctcaccaacc tggcgatgaa
      841 gtcgcgcctg ggcatggagc cggcgggctt tcgcactccc ggtggctttc gcgacgggct
      901 gcgcgagcat ccggccgtcc gtgcgatctt gaaagacttg ggctttaagt ggatcagcag
      961 cctctatcca ccgcatccca actcggccgc catggaagag ccgagcgcgg aagtgctcga
     1021 cagcattgtt gccgcgctgc ccaaggcgca gcccttcgca tattccgatg acctgatcga
     1081 agtgcccatg agcccgatca gcgacatcgg ggcgttccgc accggccgct ggcggctgga
     1141 gtggttcttg cgggccatcc gcctctgcgt ggaatggacc atcgagaacc gcgcggtctt
     1201 cgacttcctc gcccaccctt cgtgtctcta cgtcgtcgat cccgaatttc gcgcgatcga
     1261 cctgatctgc gacctggtcc gcaaggccgg cccgcgcgcg gcgctcgttg atttagggac
     1321 aatcgctaag cgcgcggtcg gtcggtaaat cggtggtcgg ttggtcggtg gattttccga
     1381 ccgactgacc gaccgactca ccgattgaga tatcccaggt cccgcacgtt ctgctccgcg
     1441 atctgccgcg tgatcaggcc cgcacggaag agctgcctga ccgattcgtc gaagctgatc
     1501 agcccttcgc ccttgctcgt caacaggtag ttgtcgatcg attcgagctt cccctgtcgg
     1561 atgccggcca cgatcggtgt gatattccag agcacttcca gggccaggtg ccgcttcgcc
     1621 ccgcgttcca gggcgggcag cagtcgctgg gcgacgacgg cccgcaggct cgaagccaat
     1681 tgataacgca catcggattg cacgtcctgt gggaacaagt cggcgtagcg gctgattgcc
     1741 cctttggcgt cgcgggtgtg cagcgtcgtg aagacgaggt ggcccgtttc ggcggcgctg
     1801 agggccatct gggccgtttc gcggtcgcga acctcgccga ccaggatcac gtcggggtcc
     1861 tgacgcaggc cgtatttcag cccgtcggcg aagctcagca cgtcggggcc gacctcgcgc
     1921 tgcgtgacca gcgagtgcgg cgcgtggggg aactggtatt ccagcggctc ctcgaccgtg
     1981 atgatccggt agccgcccgc ctgattgagc aggttgacga tcatagccag cgaggtcgtt
     2041 ttacccgagc cggtcgcccc cgtgaatatc accaggccgt cgcggaggaa cgccagcttc
     2101 tcggccagcg gccgcgggaa gccggcccat tcgaagctcg gaatcttggg cggcacgatc
     2161 cgcaggcagg ctgccaggca gccgcccgcg tggaacaggt ttgcccggaa gcggcagagc
     2221 tggccgtcca gctcggcgtc gaacgcccag tcgaggtttt tctgcgcgag cagctttgca
     2281 aacagctcgc cgggacagag ctgcgagaac atgccttgca gctccgcctc ggccagcggc
     2341 gggccggcca gctcgaccag gtcgccgtgc aggcgaatcg ccggctgata gcctgccgcc
     2401 aggtgcaggt ccgacgcccc ctgcgcaacg gcgaccgcca ggcactgctg aagcttcggc
     2461 ggcaggatct gcggtgcggg caatcggacc tcggcaaatc ggcggattac aatgaaacac
     2521 cactctcggg taaaattacc gcgcctcaaa caatctggcg actgtgctcg tcttggggac
     2581 ctgaccgatg acgaccgtag caccgccgtt gcccagtccg ccagcgcccc agccccggct
     2641 ggcatcgatc gacgcctatc gcggcctggt catgtttctg atgatggccg aggtgctgcg
     2701 attgtccgcc gtagcaaagg gcttgatcga ggccggccat cccagcgatt tctggaactt
     2761 cctggcctgg catcagacgc atgtcgagtg ggccggctgc acgctgcacg acctgattca
     2821 gccgtcgttt tcgttcctgg tgggcgtggc gttgccgttc tcgatcgcca gccgcgtcgc
     2881 caaggggcag tcacggtggc agatgctggc gcacgccgtg ctgcgggcgg tgatcctggt
     2941 gctgctcggt gtgttcctgc ggtcgatgga ctacaagcag actcgctgga cattcgagga
     3001 cacgctgtcg cagatcgggc tgggttacgt gccgctattc ttggtgggcc tgacgaggca
     3061 gcgctggcag tggctggcgc tggcggtgat tctggtcggc tattggggtg cgttcgcgct
     3121 ctacgagccg ccggtaaact tcgactatca agaggtcggc gtaccggcgg actggcagta
     3181 tcactacagt ggtctggcat cgcactggaa caaaaacagc aacctggcgt ggggctttga
     3241 cacgtggttc ctgaatctct tcccgcgcga aaaaaaacag cccttcacgc acaacggcgg
     3301 cggctatgcc acgctgagct tcattcccac gctcgcgacg atgatcctgg gactgatcgc
     3361 cggcggctgg ctgcggctgg atgaggcggc ctggaagaag gtcggctcgt tcgtcgccgt
     3421 cggcggcctc ttgatcgcgc tgggcctggg ggcacacgaa agcggcctct gcccgtgcgt
     3481 caaacgaatc tggacgccgt cctggacgct ctacagcggt ggctggtgct tcttgctgct
     3541 ggcattcttc tacacgttca tcgacgtcac cagctggact tcgtggagct tcccgctgcg
     3601 ggtgattggc gcgaattcga tagcggcgta cctgctggcg cacgggcccg accagttcat
     3661 cctccgttcg tttcgtattc acttcggcca gcagattttt gaggctttcg gcaaggaata
     3721 cgtccacgtg gtcgctggtg tagccgtgct gatcgtgcaa tggctgattc tgtattggct
     3781 ctaccggcag aagatacata tccggatctg aactgggagt catgaagatg gttcgcctct
     3841 tgtctccgct ggccctactc gcgtgtagtc tcgccgttgc cgccgagccg accagggtgc
     3901 tgattctcgt cgggccgagc acgcatccgc cgggcacgca tgaggtcgcc gccggcggac
     3961 ggctgatcga gcactgcctg gagcacatgg agaacgtcag cggcatcgag gccgaagtga
     4021 tcgacaagtg gccggacgat cggatgcggc tgcagaaggt cgccagcgtc gtcttcaccg
     4081 gcgaccgctt cccgccggcc gagatgccgg atcgcgagcg gattatggcg gatttgtccg
     4141 cgatgatgga tcacggctgc ggcctggttt gctaccacta cgccaccggc ctgggcgcga
     4201 gccacgtcac gccggaaggc gaccatccgc tgctgcgctg gatgggcggc tattttgcga
     4261 ctcgctgccc gcatcatcag tcgatcgccc gcatcttcga ctccgccacg atcgaaccgg
     4321 acggcggcga ccatccagtg ctccgcggct ggagggcgtt cacgattcgc gacgagccgt
     4381 acatcaagaa ctacttcggc aaggagggcc cggcgaagaa cgtgacgatc ctggccacgt
     4441 cgatgctgcc gccggaggct ccggcccgcg aagccgtggc ctgggccgtg tcgcggccgg
     4501 acggcggccg cggcgtcggc atcgtcatgc cacacttcta caaaaactgg cagaacgagg
     4561 atctgcggcg gctaatcctg aacggcatcg tctggtcggc gaagctcgaa gtgccgaagg
     4621 aaggcgtccg cacgacgctg ccggacctgg cgactttcaa gccggccgcc gtcgagccgc
     4681 cgccgacgaa ggcgaagtga aggattacca cgaaagacac gaaatacacg aaagtgattc
     4741 gaattctttc gtgtgtctcg tgtctttcgt ggtttgttat ctatgccaga cgaactgccc
     4801 cgctacgacc gcacgcagac gtatcgctgg aactacgacc acgcgccgga ttcgccgccc
     4861 gctgttgacg tgccgccggt tggcggatcg tggacggttt gccggcggcc ggtcggctca
     4921 ccgctgggga tctcggcggg gcccctgctc aacggccgct ggattctgta ctacgcggcc
     4981 ctcggcttcg acctgttgac gtacaagacg acccgcagcc ggtttcgcga gtgttacgac
     5041 ctgccgaact tgcagcccgt cgaaacgggg ccgattcata cggctggcgg gacgctcgcg
     5101 gcgatggacg agatgcgggg cgactgggcg atctcctttg ggatgccctc aagggagccg
     5161 gccgactggc gggccgacgt ggagtggacc cgccggcgat tgccgaagga aaaactgctc
     5221 tcggtctctg tcgttgccac gcagcagccg ggctggacgc tggatgacct ggcggacgac
     5281 ttcgcccgct gcgcgaagtg ggcgctcgac agcggcgcgg atgtggcgga gacgaacttt
     5341 tcgtgcccca acgtgacgac ggtcgatggg cagctctacc agcagccggc cggcgcgggc
     5401 ctcgttgccc agcgggtccg cgcggcgatc ggcagggccc cctactcgat caagatcggg
     5461 ctggtcgaga gcgatgacct ggccggggag ttgatcgccg cgattggtcc tttcgccgat
     5521 gccctcgcca tgaccaacac catctccgcg aaggtccgcc gcaccgatgg cgcgccgctc
     5581 ttcggcggcc agtcgcgggg catcggcggc gagtcgattc gcgccgcctc gctggcccag
     5641 gtcgcccgat tcgcccggat catcaagcag cagcggctac cgacgcgaat caccggctgc
     5701 gggggcattt cttcggccga gcacgtccgc gactatctcg ccgcgggcgc cgaatcggtg
     5761 cagctcgcca ccgccgtcat ggtcgatccg ctcgtcggct gcaagatccg gcgagagctg
     5821 taagtgatta cttcgaccgc gggttccgca agaagtacag ccggccgtct tccgcgccgc
     5881 cgaggaaatc gggaatgccg tcgccgtcga aatcgacggt ggcggggctg acgtcgtggc
     5941 cttcgatgtt cgtcggggcg agcgcgccgg cgttctggaa gacccactgg ccgccgcgct
     6001 ggccgatttg ccgcacgaaa tcggcgttgg ccgagttcag caggatatcg aaccgcccgt
     6061 cgccgtccca gtcggtgacg cacagcttcc gccgcccgct ggcgcccgcc gccctcggat
     6121 tcagccacag cggcttgccg ccttcgtcga cgaaggcgcg gcggggcgct cgcggtgtca
     6181 gcctgccgtc caacttcgcc ctctcgaaca gcgccagata gccctcctgg tcgagcactg
     6241 ccaggtcgag caggccgtcg cccgtgaagt catagacaac cggcgtcgtc cgccactgcg
     6301 tgaccagttc cttcccctgc aggtgccacc agacccaggc gggcttcggc gtcggtccgg
     6361 cccactcgac ctcgatcggc tgcggctcgg cgagccgcgg cgcggtccgc gtgccgacgt
     6421 tcctcagcca ctgcaccttg ccccagatcg agttgaagac gatgtccggc aagccgtcgg
     6481 cgtcccagtc ggccacgttg agggtcgtgt atccccactt ggcttcagcc gggccctgca
     6541 cactgccatt ttcgccggcc atgacgcgaa acgtcttgcc gccggcctct agccgccgcg
     6601 gggcggccca ttttggctct gcgaccttca gcccgctcag gttttcgaac aactcgatgt
     6661 agcccgcggt gttgccgcaa acaatgtccg tgtcgccgtc gccgtcccaa tcgcaaccaa
     6721 agggcgtggc cagtgcgccg cacttgagcg cgtccgcttc c
//
LOCUS       DASRJB010000452         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3507294, whole genome shotgun sequence.
ACCESSION   DASRJB010000452 DASRJB010000000
VERSION     DASRJB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3507294"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB80_11915"
     CDS             complement(<1..574)
                     /locus_tag="VFB80_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34523.1"
                     /translation="MTRSAYYGFALLCSLPAFAVAQQPAGDQPVNTWVQQSPRDGKPS
                     PNFPYEGSGAYDPIGKRWIHHAGHDGIPQSFHTFTFDLDGGAWEQKFPPTSPPGVCCV
                     DGTNCFDTANRRFVRFPGGSLGHGFQWSRGVKLKESAVWLYDPVTNGWTNMRPGPYGQ
                     PDSRQTVGGLDSGATYDPEHELAYTFGGQSS"
     gene            1195..>2065
                     /locus_tag="VFB80_11920"
     CDS             1195..>2065
                     /locus_tag="VFB80_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN34524.1"
                     /translation="MPHAIICRQLVKRYPGKPPVMAVRGLDLAVEEGECFGLLGPNGA
                     GKTTTMEIVEGLLDATSGEVEVLGRRWDHNGDEIREQIGISLQETRLADKLTVRETVT
                     LFRSFYQSGLTPDEAIGRVSLEEKVNSYVVKLSGGQQQRLAVACALVGDPRLLFLDEP
                     TTGLDPQSRRQLWEVIGQFRKTGRTVMLTTHYMDEAERLCDRVAIVDHGQTIALGSPA
                     QLIAQLGGENIVDFALAGGSPPLSEAELAQLPAVRTARCQGETYSLAVTQPHVALPAL
                     LGLLQSRGLALAHL"
BASE COUNT          376 a          636 c          686 g          367 t
ORIGIN      
        1 tggaggactg cccgccgaac gtataagcca gctcgtgctc gggatcgtag gtcgcgccgc
       61 tatccagccc gccaaccgtt tgccgcgaat ccggctggcc atacggccca ggccgcatgt
      121 tggtccagcc gttggtcacc gggtcataga gccagaccgc cgactccttc agcttgacgc
      181 cgcggctcca ctgaaacccg tggccgagcg agccgccggg gaagcggacg aaccgccggt
      241 tcgccgtatc gaagcagttc gtgccgtcca cgcagcagac gccgggcggc gaagtcggcg
      301 ggaatttctg ctcccacgct ccgccgtcga gatcgaacgt gaacgtgtgg aagctctgcg
      361 ggatgccgtc gtgccccgcg tgatgaatcc accgcttgcc gatcgggtcg taggccccgc
      421 tgccctcata ggggaaattt ggagaaggct ttccgtcgcg cggactctgc tgcacccagg
      481 tattcaccgg ctggtcgccc gcgggctgct gggcgactgc aaatgcgggg agcgagcaaa
      541 ggagtgcgaa cccatagtag gcgctgcgtg tcatgggcag ttctccggca aggcttgttg
      601 gacgtatcgt aatgcgccga tgcgaccggc agcaaaccgt tggcagaagg atcgagtcaa
      661 atgaaaacgg acccgcaaaa aacacccaag attgcggcaa accgtacgat actttctatg
      721 tggactggcg tttaacacgt gaggcattct cgggtgaggt cgcgatcgaa cgagcgttgt
      781 tttgccggga ttttgtgttc tttacaacta tagtggatgg atgtttaata agggcgaaga
      841 ttctgccgtt gcaccgccgg ccgacgctcg cgcgcgagag gggctgcggg ggtaaggata
      901 tttcccgaag gacctctcta acgcttttaa ctcaaacggc atttggcgag agtttattgg
      961 acgtaaacgc tcgccgcgga aggggttagg attggaattc gagcgtccaa ggggacgcca
     1021 gccggccgaa gcacaagcaa ttatccttac ctgacttctg acgacatcgg cgggcgatgg
     1081 ctttcctgct aagggcaaag tcagggcggc gtcgctcgct gagtcgcggc caggtttggt
     1141 tgccgcccgg acttggcggt ttcgcagcgg gctgtcatcc tgatgggttc gcctatgcct
     1201 cacgccatca tctgccgaca gctcgtcaag cgttatccgg gcaagccgcc cgtgatggcg
     1261 gtgcgggggc tggatctggc ggtcgaggag ggggagtgct ttggcctcct ggggccgaac
     1321 ggcgcgggca agacgaccac gatggagatc gtcgaggggc tgctcgacgc caccagcggc
     1381 gaagtcgaag tgctcggccg ccgctgggac cataacggcg acgaaattcg cgagcagatc
     1441 ggtatctcgc tgcaggaaac gcggctggcc gacaagctga ccgtccgcga gacggtaacg
     1501 ctgtttcgca gcttctatca atcgggcctg acgccggatg aggcgatcgg ccgcgtctcg
     1561 ctggaggaaa aagtcaacag ctacgtcgtc aagctctccg gcgggcagca gcagcggctg
     1621 gccgtggcct gcgccctcgt cggcgatccg cggctgctgt tcctcgacga gccgaccacc
     1681 ggcctcgacc cgcaatcgcg gcggcagctc tgggaggtga tcggccagtt ccgcaagacc
     1741 ggccgcacgg tgatgctcac gacgcactac atggacgaag ccgagcggct ctgcgaccgc
     1801 gtggcgatcg tcgatcatgg gcagacaatc gccctcggct cgccggccca gctcatcgcc
     1861 cagctcggcg gcgagaacat cgttgacttt gccctcgccg gtggatcgcc tccactaagc
     1921 gaggccgagc tggcgcaact gcccgcggtc cgcacggccc gctgccaggg cgaaacgtac
     1981 agccttgccg tcacgcagcc gcacgtggcg ctgccggcac tgctcgggct gctgcagagc
     2041 cgcggcctgg ctctggccca cctga
//
LOCUS       DASRJB010000453         9885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_125630, whole genome shotgun sequence.
ACCESSION   DASRJB010000453 DASRJB010000000
VERSION     DASRJB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125630"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFB80_11925"
     CDS             <1..332
                     /locus_tag="VFB80_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34525.1"
                     /translation="TDGTSNTFLFLEAGHYKNQSWLPRLKGANHFIWTHHASQGYVQS
                     RTNANVLSPPNDTQNNTRAPASQHPSGVQATMVDGHVVWVSNHIDSVIYENTYSRLGG
                     EPNGGQF"
     gene            378..806
                     /locus_tag="VFB80_11930"
     CDS             378..806
                     /locus_tag="VFB80_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34526.1"
                     /translation="MFRSLVWLGVCVSLAMLLGCRSDAPDIVEIEGTLTYKGKPIPNI
                     KIFFCPTTGRPSWAVTDQNGHFVLDYDEQFDGAKVDTHTVYFVDEGGMVDPTAALSGA
                     APPKRSPDAAELVRKYNSRETSTLKVEVKERNKNFKLDVN"
     gene            822..1727
                     /locus_tag="VFB80_11935"
     CDS             822..1727
                     /locus_tag="VFB80_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN34527.1"
                     /translation="MPRLAAFPKAWMDPLCKTGTLTLWAWIELASTLEIDGLEFYAGF
                     LDLADEANWQPARQAAEAAGLAIPMLCCSPDFTHPDADFRRAQVQQEKSWITMAAALG
                     AKFCRVLSGQRRPEVSREEGLAYAADAIQACLPHAEWCGVTLVIENHYKDNYWQYPEF
                     AQEMDVFCELVARIDSPRFGVNYDPSNTLLAGEDPLELLRRVKHRVRTMHASDRYLAE
                     GTLADLRREESSVGYAKRLRHGVIGRGLNDYDAIFRELASAGFDGWISIEDGENGLDE
                     LAASVAFLRQKIAAHWPIADGGRAG"
     gene            complement(1756..2070)
                     /locus_tag="VFB80_11940"
     CDS             complement(1756..2070)
                     /locus_tag="VFB80_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34528.1"
                     /translation="MKRLLLAAVCAASLAVPLGCNRGTPTVESVQAPAPDPLAEAKAI
                     LTNYAKGRPVTSEAESFADLAQRVKDKDPAKGELLDKGLQSIKANPASARSKATELLK
                     KL"
     gene            complement(2095..3156)
                     /locus_tag="VFB80_11945"
     CDS             complement(2095..3156)
                     /locus_tag="VFB80_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34529.1"
                     /translation="MNAYPRSRGFTLVELLVVIAIIGVLVALLLPAVQSAREAARRMQ
                     CSNNLKQLGLGIHNFHDTFNLFPYNYQLIGTNAWEAASAHYQILPYIEQQNLQSQFQI
                     PPTSRPGQPAGAAAGDAAMWTSSWNGPMNTPVKTFLCPSALKGPTRAQVIWGGPGSNY
                     GWCTGSRVQVVWVGSAFNGMIAYQHERRMKDVTDGLSNTVLASEFLGGSGTNFGTAKY
                     PFDVFYAGDAPFNAVADKDFPTPAELDNIGAAALRMDGGGFRGNVGGSWAWYAAGHST
                     FNTAATPNWKYPTAGGACCPGGAQDWFDGVIPARSMHPNGVTALLGDGSVRFVINTVN
                     LLTWQRLGARDDGQALGEF"
     gene            complement(3322..4668)
                     /locus_tag="VFB80_11950"
     CDS             complement(3322..4668)
                     /locus_tag="VFB80_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34530.1"
                     /translation="MRSWYSLAATVLLATISSARAQSPTALANWGQWRGPLGTGASPQ
                     GNPPTEWSETKNVQWKVPIPGHGTSTPVIWGDKVFVQTALSARSSAGSSSAKGDAPAK
                     KGKGGFGGAPPPTEPYRFVLMCLDRNTGKTLWEKVCREQVPHEGFRPGDGSFAAASAI
                     TDGQSVFAFFGSRGLFCFDMSGALKWEKDLGDQETRNDFGEGATPALHGNTIVVPWDH
                     EDEDFVVALDKRSGDELWRQPRDEPTGWATPLIAEHGGSPQVITIGTNRTRSYDLASG
                     KQIWETEGLTANVIPTPVAAGGVAYVTSGFRGSKLLAIKLGATGDITGTPAVLWRLDQ
                     DTPYVPSPLLSGQRLYFFKSNNNILTCLDAASGKPHYSAQRIDGLGTVYPSPVAAAGR
                     VYLTDRRGKTVVIKDANQLEVLATNSIAEPIDASPAVVGKQMFLRSHSSLYCIAEK"
     regulatory      4902..5099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5229..7469
                     /locus_tag="VFB80_11955"
     CDS             5229..7469
                     /locus_tag="VFB80_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN34531.1"
                     /translation="MSLLVFGFGAPLAQISNVISTIGISGVVLIGAAFALLLAAWLSL
                     RFIPNHSVGVVEKLWSAKGSVPEGQILALKGEAGFQADLLRGGIHFGLWRWQYRVHLC
                     PLVTVSQGKIGYVYARDGQPLSPSQTLCHAVACNNFQDARAFLQGDEKSQRRGQRGRQ
                     LVIVREGVYAVNPALFVVITENAAYTLRSLLTPHELATVRSWQEELSEIGGFAPVVIG
                     QLVKAANPLHPEEPMTVDSIGIVTVHDGPSLAVGEIIAPAVGADVGETNYHNNFQDPQ
                     RFLDGGGRRGRQYQPITDGTYFINRWFATVELIPKTVVPIGYVGVVVSYYGQAGDDLS
                     GIAFRHGERVAEGRRGVWERPFGPGKYAFNTYAGNIVLVPTTNFVLHWVTGKSEAHRY
                     DESLRSIDLVTRDAYEPRLPLSVVVHIDYQRAPNVIQRFGDVKKLITQTLDPMLSAYF
                     RDIAHKKTMLELLHERDLIQTEARAELLRKFREFDIECVDVLIGKPDTEEQGGKIEVL
                     LEQLRTRQLSIEQLETYQRQREASDKRRELAEAQAQADMQTSLTNTRVKAQIAESEGE
                     AELQRARKQAEKQIVMAEANLAQSRRQAEQTLVLAEADSQQAILAGKGEAQRIMQVGL
                     SEAAVLMQKVSSYGDPRLFALTHVGSALSKSTQPLVPERVFLSGGGSDSPSGNGHDGA
                     SIATTGLVGMLLQTLLAERTSFANGNSEDLAALKELTSRLTRETVANMPCAQPPAEAK
                     EIAAAN"
     gene            complement(7898..8107)
                     /locus_tag="VFB80_11960"
                     /pseudo
     CDS             complement(7898..8107)
                     /locus_tag="VFB80_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            8401..9504
                     /locus_tag="VFB80_11965"
     CDS             8401..9504
                     /locus_tag="VFB80_11965"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34532.1"
                     /translation="MKSAKRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRIHCG
                     NNMKNLGLAFHNYNDTFNRFPFASVAHPFDFFAPTATASFRDTPGTAVPAAPGWGTTW
                     GIALLPFVEQQNRYELWNNSLGYGSTANQRVVTGTPLLVMKCPSDPKAPAAVNPDNNL
                     GTFDKGNYGYNFGGGFANENGNANGGGPLNSPSWTALPPPNGYGMGSMNRGLASLRDD
                     FLRQLPTSVRLSEIQDGTSNTILLGEMLKSPSNEDCRGCWGKALGAAVSAYTLAFPED
                     GSIGIATPNVRAVGDFRDGPTHCNAPATDLQLACTDRAGDSRGGNAMRSRHPSGAQAV
                     FCDGRVSFFSNSVDRLAYRAAFTIQGSETINSN"
     gene            9515..>9885
                     /locus_tag="VFB80_11970"
     CDS             9515..>9885
                     /locus_tag="VFB80_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34533.1"
                     /translation="MKTTTDARIFLATSAIALCLAGCGQRSGRPDLDAIPAEELHGAS
                     TKGLLYEFRAKVRKYGVTAAKKELPTVLEAFADYEQQPIGSHKDTYRQIAEKLKALET
                     TLAGSPTKDAAIQAADEIGALA"
BASE COUNT         1807 a         3238 c         3139 g         1701 t
ORIGIN      
        1 tcaccgacgg aacgagcaat acgttcctgt tcctcgaggc cggccattat aagaatcaga
       61 gttggctccc gcggctgaaa ggcgctaatc actttatttg gactcaccat gcctcgcagg
      121 gctatgtcca gtcgcgaacg aacgccaatg tcctctcgcc tccgaacgac actcagaaca
      181 atacccgcgc tccggcgagc cagcatccat cgggcgtgca ggccaccatg gtggatggcc
      241 acgtcgtttg ggtcagcaat cacatcgata gcgtcatcta cgagaacacc tattcgcgac
      301 tcggcggcga accgaacggc ggccaattct gaaatctgac ccgcaccttg agtctttcat
      361 tcccggaagg agcagtgatg ttccgctcat tggtgtggct gggcgtttgt gtttcgctgg
      421 cgatgcttct gggatgccgc tccgacgctc ctgacatcgt cgaaatcgaa ggcacattga
      481 cctacaaagg caaaccgatt ccgaacatca agattttctt ctgccccacc accggtcggc
      541 cgagctgggc agttacggat cagaatggcc acttcgttct cgactacgat gagcaattcg
      601 acggcgccaa ggtggatacg catactgtct atttcgtgga cgaaggcgga atggtcgacc
      661 ccacggcagc cttgagcggt gcggcgccac caaaacgctc gccagatgcg gccgagctgg
      721 ttcgcaaata caattctcgc gagacatcaa cgctcaaggt cgaggtcaaa gagcgaaata
      781 agaacttcaa actcgatgta aattgagcat ggggctccgc tatgccccgt ctggccgcgt
      841 ttccgaaggc ctggatggac ccgctgtgca agacggggac gctcacgttg tgggcctgga
      901 tcgagctggc cagcacgctg gaaattgacg ggctggagtt ttatgccggg ttcctggacc
      961 tggcggacga ggccaattgg cagcccgcgc ggcaggcggc cgaggcggcg ggtctggcga
     1021 tccccatgct ctgctgctcg ccggacttca cgcatccgga tgctgacttt cgccgcgctc
     1081 aggtccagca ggaaaagtcc tggatcacca tggccgcggc gctcggcgca aagttctgcc
     1141 gcgtcctgtc cggccagcgg cggcccgaag tcagccgcga agagggcctg gcctacgccg
     1201 ccgatgctat ccaagcttgt ctgccgcacg ccgagtggtg cggcgtgacg ctggtgatcg
     1261 aaaaccacta caaggacaac tactggcagt atccggaatt cgcccaggag atggacgtct
     1321 tctgcgagct ggtcgcgcgg atcgattcgc cgcgcttcgg cgtcaactac gatcccagca
     1381 acacgctctt ggccggcgag gacccgctgg aattgctccg ccgcgtcaag caccgcgtcc
     1441 gcacgatgca cgccagcgac cgctacctgg ccgaggggac gctcgccgat ctgcgccgcg
     1501 aagaatcgag cgtcggctac gccaaacgcc tccgccacgg cgtcatcggc cgcggcctca
     1561 acgattacga cgccatcttc cgcgagctgg cctccgccgg cttcgacggc tggatcagca
     1621 tcgaggatgg cgagaacggc ctggacgagc tcgccgccag tgtcgccttc ctccgccaaa
     1681 aaatcgccgc gcactggccg atcgcggacg gtgggcgtgc cggctgattc tcggcccgct
     1741 gggcggcgaa tcgccttaca acttcttcag cagttccgtg gccttggacc tggctgaagc
     1801 cggattggcc ttgatcgact gcaagccctt gtcgagcagt tcgcccttgg cggggtcctt
     1861 gtccttcacg cgctgggcga gatccgcgaa cgactccgcc tcgctggtga cgggccggcc
     1921 cttggcgtag ttcgtcagga tcgccttggc ttcggcgagc ggatctgggg caggcgcctg
     1981 aaccgattcc acggttggcg ttccacgatt gcaacccagc ggcacggcca gcgaggcagc
     2041 acacacggcg gccagcagca atcgcttcat aacgtccccc gccgcatgtg gcgcttagaa
     2101 ttcgcccaac gcctgcccgt cgtcgcgagc gcccaggcgc tgccacgtga gcagattgac
     2161 ggtattgatg acaaaccgta ccgagccgtc gcccagcagc gcggtgaccc cgttggggtg
     2221 catgctccgc gcgggaatca cgccgtcgaa ccagtcctgt gcgccgccgg ggcagcacgc
     2281 gccgccggcc gtgggatact tccagttcgg cgtggcggca gtgttgaagg tcgaatggcc
     2341 cgccgcgtac caggcccagc tgccgccgac gttgccgcga aagccaccgc catccatccg
     2401 cagggccgcc gcgccgatgt tgtccagctc ggccggcgtc ggaaaatcct tgtcggcgac
     2461 ggcattgaac ggcgcgtcgc ccgcgtagaa cacgtcgaac ggatattttg ccgtgccgaa
     2521 gttcgtgccg ctgcctccga gaaactccga cgccagcacc gtgttgctca ggccgtcggt
     2581 cacgtccttc atccgccgct cgtgctggta ggcgatcatc ccgttgaacg cgctgccgac
     2641 ccaaaccacc tgcacgcggc tgcccgtgca ccagccgtag ttgctgccgg gcccgcccca
     2701 gatcacctgg gcccgcgtcg ggcccttgag cgccgagggg cagaggaacg tcttaacggg
     2761 cgtgttcatc gggccgttcc agctggaggt ccacatggcc gcgtcgcccg cagcggcgcc
     2821 ggccggctgg ccgggccggg acgtcggcgg aatctggaac tggctctgga gattctgctg
     2881 ctcgatatac ggcaggatct gatagtgggc gctggctgcc tcccaggcgt tcgtcccgat
     2941 cagctgatag ttgtagggga acaggttgaa cgtgtcgtga aagttgtgga tgcccaggcc
     3001 gagctgcttg aggttgttcg agcattgcat ccgccgggcg gcttcgcggg ccgactgcac
     3061 ggctggcagc agcaaggcca ccagcacacc gataatggcg atcacgacga gcagctccac
     3121 gagagtaaag ccgcggctgc gtggatatgc attcatggtt ccctccaaga aatgaataga
     3181 cgcaagtccc ttgcgttcag ttccgcggcg aaatcaccac aaatccccta gaagtggggc
     3241 tgaaagggtt ctgaatcgtg aggggccata cagtattcgc ttggcggcga ggcgtcaatg
     3301 ccagttagct ggctgctggc gctacttctc cgcgatgcag tacaagctgg aatggctgcg
     3361 gaggaacatt tgcttgccga cgacggcggg cgaagcgtcg atcggctcgg cgattgagtt
     3421 tgtcgccaaa acctccagct ggttggcgtc cttgatcacg accgtcttgc cgcggcgatc
     3481 ggtgagatag acgcgccctg ctgcggcgac gggagaggga tagacggtgc ccagcccatc
     3541 gattcgctgg gcgctgtagt gcggcttgcc gctggcggca tcgaggcatg tcaggatgtt
     3601 gttgttgctc ttgaagaaat agagccgctg gcctgaaagg agtggcgaag gtacataggg
     3661 agtgtcctga tcgagccgcc ataggacggc gggcgtgccg gtgatgtcgc cggtcgcgcc
     3721 gagcttgatc gccaggagct tgctgccgcg aaagccgctg gtcacatagg cgacgccgcc
     3781 cgcggcgacg ggcgtgggaa tcacgttggc ggtcagcccc tcggtctccc agatttgctt
     3841 gccgctggcc aggtcgtagc tgcgggtgcg gttggtgccg atcgtgatga cctgcggcga
     3901 gccgccgtgc tccgcgatca gcggcgtggc ccagccggtc ggctcgtccc gcggctgccg
     3961 ccacagttca tcgccactgc gcttgtcgag ggccacgacg aagtcctcgt cctcatggtc
     4021 ccagggcacg acgatcgtgt tgccgtgaag tgcgggcgtg gcgccttcgc cgaaatcgtt
     4081 gcgggtctct tgatcgccga ggtccttttc ccatttcagc gcgccgctca tgtcgaagca
     4141 gaacagcccg cgcgacccga aaaaggcgaa tacgctctgg ccgtccgtga ttgccgacgc
     4201 cgcggcgaag ctgccgtcgc ccggacgaaa gccctcgtgc ggcacctgct cgcggcagac
     4261 tttttcccag agcgtcttgc ccgtattgcg gtcaaggcac atcagcacga agcgatacgg
     4321 ctcggtgggc ggcggtgcgc ctccgaatcc gcccttgccc ttcttcgccg gtgcatcgcc
     4381 tttggccgac gacgagcccg ccgagcttcg cgccgacagc gccgtctgca cgaagacctt
     4441 gtcgccccag atcactggcg tagaggtgcc gtggccgggg atcggcacct tccactggac
     4501 gtttttggtc tcgctccact cggtgggtgg atttccttgc gggctagcgc cagtaccgag
     4561 cggcccgcgc cactggcccc agttggccag agccgtgggt gactgggcgc gagcggatga
     4621 aatcgtggcg agcagcacag tcgcagcgag cgaataccaa gatctcacga gcaagtcctc
     4681 ccaagatgtc gaaacttgtc cgaacaagtt ctaacacgcg aagatgacga ttgtggcggc
     4741 tgctttttgc attaacgtct cgaaataaaa agcccctcta ctgggccgac ccccactaac
     4801 ggcggttgac ggaaccgcaa tcccgacagc cagttgcgcc caaacgccac cttggcgtcc
     4861 cgtttgcacc ccaaggaagg cctagaattg acgatataat ctctgactag cagcctaatc
     4921 ctgcatgcaa gtgcgggagc gggggaacca atgagttggc gagtagccaa ctccggggcg
     4981 caggttggag cggcccagcg agccgcaagg cagacacgaa tgtctgcccc acattccagc
     5041 cagggtactt tcaagcccta gcccgacagc taacctcgtc ggcggactgc ggcgtgtgcc
     5101 gccgtccaga attgaaaggg catcttcgtc cgccgctcgg acagatcgcc cccagagttt
     5161 tgacgtaggg tatcctgcta cgcgctgcgc gcggctatga cgcaggttct gccctacagg
     5221 aggcggttat gtctctccta gtctttgggt tcggcgctcc cctagcgcaa atctccaatg
     5281 tgatcagcac catcggcata tccggcgtgg tgctgatcgg cgcggccttc gcgctgctgc
     5341 ttgcagcctg gctcagcttg cggttcattc cgaaccactc ggtgggcgtg gtcgagaagc
     5401 tgtggtccgc caagggctcg gttcccgagg gccagatcct cgctctcaag ggcgaggccg
     5461 gctttcaggc cgatctgctc cgcggcggca tccactttgg cctctggcgg tggcagtacc
     5521 gcgtgcactt gtgcccgctg gttacggtgt cgcagggcaa gatcggttac gtctatgccc
     5581 gcgacggcca gccgctgtcg ccgagccaga cgctatgcca cgcggtcgcc tgcaacaact
     5641 ttcaggatgc ccgagcattc ctgcaaggcg atgaaaagtc ccagcgccgc ggccaacgcg
     5701 gccgccaact cgtcatcgtt cgcgaaggcg tgtacgcggt caacccggcc ctgttcgtgg
     5761 tgatcaccga gaacgccgcc tacacgctgc ggagcctcct gacgccgcac gagctggcca
     5821 ccgtccgctc gtggcaggaa gagctctccg agatcggcgg ctttgcgccg gtcgtgatcg
     5881 gccaactggt caaggcggcg aacccgctgc atccggaaga gccgatgacg gtcgatagca
     5941 tcggcatcgt gacggtgcat gacggtccgt cgctggccgt gggcgagatc attgccccgg
     6001 cggtcggcgc cgacgtcggc gagaccaact atcacaacaa cttccaggat ccgcagcggt
     6061 tcctggacgg agggggccgc cgcggccggc agtatcagcc gatcaccgac ggcacctatt
     6121 tcatcaaccg ctggttcgcc acggtcgagc tgattcccaa gacggtcgtg cccatcgggt
     6181 acgtcggcgt ggtggtcagc tattacggcc aggcgggcga cgacctgtcg ggcatcgcgt
     6241 ttcgccacgg cgagcgcgtg gccgagggcc gccgcggtgt ctgggagcgg cctttcgggc
     6301 cgggaaaata tgccttcaac acttatgccg gcaacatcgt cctggtgccc accacgaact
     6361 tcgtgctgca ctgggtcacc ggcaaatccg aggcccatcg ctacgacgag tcgctgcggt
     6421 cgatcgacct ggtgacccgc gacgcttacg agccgcggct gccactgtcg gtggtggtgc
     6481 acatcgacta ccagcgggcc ccaaacgtca tccagcggtt cggcgacgtc aagaagctga
     6541 tcacgcagac gctcgacccg atgctctcgg cctacttccg cgacatcgcc cataagaaga
     6601 cgatgctgga gctgctgcac gagcgcgacc tcattcaaac cgaggcccgc gcggagctgc
     6661 tgcggaagtt ccgcgagttc gacatcgaat gcgtcgatgt gctgatcggc aagcccgaca
     6721 ccgaagagca gggaggcaag atcgaggtgc tcctcgagca gctccgtacg cggcagctct
     6781 cgatcgagca gctcgagacc taccagcggc agcgcgaggc gtcggacaaa cgccgcgagc
     6841 tggccgaggc ccaggcccag gccgacatgc agaccagcct gaccaacacc cgcgtgaagg
     6901 cccagatcgc cgaaagcgaa ggcgaggccg aattgcagcg ggcgcgcaag caggccgaga
     6961 agcagatcgt catggccgag gccaacctcg cccagtcgcg gcggcaagcc gagcagacgc
     7021 tcgtcctggc cgaagccgac agccagcagg cgatcctcgc cggcaagggc gaggcccagc
     7081 ggatcatgca ggtcggcctc tccgaggccg ccgtgctgat gcaaaaggtc agcagctacg
     7141 gcgacccgcg gctgttcgcc ctcacgcacg tcggcagcgc gctctccaag agcacgcagc
     7201 cgctcgtgcc cgagcgggtg ttcctgtcgg gcggcggaag cgacagcccc agtggcaatg
     7261 gccacgacgg tgcgtccatc gccacgaccg gcctcgtcgg catgctgctg caaacgctcc
     7321 tggccgagcg gacgagcttc gctaacggaa acagcgaaga cctcgccgcc ctcaaggagc
     7381 tgaccagccg gctgacgcgc gagaccgtgg ccaacatgcc ctgcgcgcag ccgcccgctg
     7441 aggcgaagga gatcgccgcg gcgaactgat cgtcacgaat gctgggcagc gtgcggcaaa
     7501 ttgctgcacg ctgcccggtg gcgaacgact acagtcccat cagcaatgcc gccgccgggc
     7561 caacggcggc tcgggtgacc tggtcgcgca gctcctggag ccgggccatg ccgccgtcca
     7621 tcatcccgtt atccggattg gccaggacgt cgagcaggaa caggatgtga tcctcggcca
     7681 gcagtacgtc ctgcacgccc tgcatcgcga accgcaaggt ccgccgatcg gcgtcggtaa
     7741 gcatggggtc ttgcagcgcc ttctcgatgt cgccggcgac atcctcgaag ttggccctgc
     7801 tattctcgaa ttccatgtgc aagcggcgca cctcgtccag attgacttca cccgcgcgga
     7861 gcatcgcatg aaacgcgaca agcgagtcgc ggagcagcat cgaggcggaa tcgtgatagt
     7921 tgtgcagcgc cagtccgatc tgcttgaggt tgttcgacgc ctccgcccga gccgccgccg
     7981 cccgcacctt atgcactgca ggcaagagca ggccgatcag aatcgcgatg atggcaatca
     8041 cgaccagcag ctcgatcagc gtgaatcccc ggctgcgagt gcggcgcact agaagagcac
     8101 gcgacatgga tcacctcctg cacgtgcgag taaagaataa aagggaaacg ccgagcgcga
     8161 ttatcaaacc ctcccgctgc gcccgtcaac cagagcactc gtccgaggcc agtgggtccg
     8221 ccgcgacgat tcttgaccgc cgcccgcctg cgctgttagc gtgaaagccg gcggaggaat
     8281 gtttcgcatg ctcctcccgg cgaggggcgc gagcgcactc gataagcagg aatctttctc
     8341 cgtctccgtc tccgcagggg cggttggttg gctcgatcct ggcaatcgcg ggggtgtctc
     8401 atgaaatccg caaagcgggc cttcacgctg gttgagttgc tggtggtcat cgcgatcatc
     8461 ggcgtcctgg tggcgctgct cttgcccgcg gtgcaggccg ctcgcgaggc cgcccgccgc
     8521 atccactgcg gcaacaacat gaagaacctg ggcctggcgt tccacaacta caacgacacg
     8581 ttcaacaggt tccccttcgc atcggtcgcc catccgttcg acttttttgc tcccacggcg
     8641 acggcgagct ttcgcgacac gccgggcacc gccgtccccg cagcgcccgg ctgggggacg
     8701 acctggggca tcgccttgtt gccattcgtt gagcagcaga accgctatga gctgtggaac
     8761 aacagcctgg gctacggcag cacggcgaat cagcgggtag tgacgggaac gccgctgctc
     8821 gtgatgaaat gccccagcga tcccaaggcg cccgcggcgg tgaacccgga caacaacctc
     8881 ggcacgttcg acaagggcaa ctacggctac aactttgggg gcggcttcgc caacgaaaac
     8941 ggcaacgcca atggcggcgg acccttgaac agtccgagct ggaccgctct gccgcctccc
     9001 aatggctacg ggatgggctc gatgaaccgc ggcctggcct cgctccgcga cgacttcctg
     9061 cggcaattgc cgacgtccgt ccgcctcagc gagattcagg acggcacgtc caacacgatc
     9121 ctgctcggcg aaatgctcaa aagcccgagc aacgaagact gccgcggctg ttgggggaaa
     9181 gctttggggg ctgcggtcag cgcttacacg ctggcctttc ccgaggatgg ctcgatcggc
     9241 atcgcgacgc ccaacgtccg ggcagtcggc gactttcgcg acggcccgac gcactgcaac
     9301 gccccggcga ctgatctgca actggcctgc accgacaggg ccggcgattc ccggggcggc
     9361 aacgcgatgc gcagccgcca tcccagcggc gcgcaagcgg tgttctgcga cgggcgggtg
     9421 tctttctttt ccaacagcgt cgatcgcctg gcctatcgcg cagctttcac gattcaaggc
     9481 agcgagacga taaacagcaa ttaaggggcc gcgcatgaag acgacgacgg acgcgcgcat
     9541 cttcttggcg acgagcgcca ttgcgctctg ccttgccggc tgcggccagc ggtcggggcg
     9601 gcccgacctg gatgcgattc ccgccgagga gctgcacggg gcatcgacca agggcctgct
     9661 ctacgagttt cgggccaagg tccgcaagta cggcgtgaca gccgcgaaga aggagctgcc
     9721 cacggtgctc gaggccttcg ccgactacga gcagcagccc atcgggagcc acaaagatac
     9781 ctaccgccag atcgcggaga agctcaaggc cctcgaaacc actctcgccg gttcccccac
     9841 caaggacgcc gccatccaag ccgccgacga aatcggcgcc ctcgc
//
LOCUS       DASRJB010000454        10233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2947295, whole genome shotgun sequence.
ACCESSION   DASRJB010000454 DASRJB010000000
VERSION     DASRJB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947295"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1087
                     /locus_tag="VFB80_11975"
     CDS             221..1087
                     /locus_tag="VFB80_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34534.1"
                     /translation="MPISYSCPHCGKQFSVAEQFAGQTGPCAACGKPISIPLSGGGSG
                     AAVAAAGVGIGATLMIVAGAAIMGLLLCAGLLVALLLPAVQAAREAARRMQSMNNMKQ
                     ISIALLNYHDVYGTFPPAVVTDASGKPLYSGRVLLLPFLEQDPMFQQFRKDEAWDSPS
                     NLPLTQQSLKIFQDPSAPPSASGQTDYLFVTGTGTAMEPKPGGIKMNDIVDGTSNTMA
                     MVEFKGSGINWAQPRDFDVTQPTALPPGNHPGGNLVMMYDGSVRFVSKNVSPQLIQQM
                     STRAGGEVVSPP"
     gene            1183..2055
                     /locus_tag="VFB80_11980"
     CDS             1183..2055
                     /locus_tag="VFB80_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34535.1"
                     /translation="MAIAYSCPHCSKQFSVAEQYAGQTGPCAACGKPITIPMAGYAYA
                     PKAGAAAAAGGGIAVVAIVLVVMLVLCAGVGLALLLPAVQAAREAASRSQSSNNLKQI
                     GLALHNYHDVYGAFPPAVVTDASGKPLYSGRVLLLPYLEQNNLYQAFNKDEPWDSPNN
                     RIISQTPIRTFMDPSSPERPATAGKTDYLFVTGQGTIFEAGKNTKLMQITDGTSNTIL
                     CIEVKNSGVNWAEPRDFDASQPVPLPPGNHPNVNIVLFADGSVRTLSQSAASPPLVRQ
                     ISSKDGGEAVNVPY"
     gene            complement(2142..4397)
                     /locus_tag="VFB80_11985"
     CDS             complement(2142..4397)
                     /locus_tag="VFB80_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN34536.1"
                     /translation="MKLAQSHRLAPPGSAAGAEPRLTSWRVSTGGMATAESKAASADS
                     ELQHSFGLGGVGFDFLPLELPGVDPSLPTGPQTKLPVIGLESAGPLPVVDDRGADAPR
                     SPAEPAPNRGPGEIWLDGDVILCVCPDCSAPMSVRFWLMLADCWRCGASIELSEEQER
                     QVQQLLAERQRQAAAPAQAPRPPAPRASAAKNDSPKQPLAPPARPAAERFRGAPRPEP
                     AAPARPRPPRPEPPAQSPPAQPAAPTSAPPARNSAHHAGRTNNVPPPRVLHHEPWLKH
                     LLNDTPAWFISMLLHLVAFTVLALLFTDEESSIFGRNILLSTNYGRNRTEGGDTIAYP
                     PDERAEFDLPLPSKADLNDNRQRNVLLAAAQDARELRLDDDTPNLPNLQAVKVAIGRA
                     DGFTHAVASRDPRLRTELVKQEGGTTLTEAAVARGLRWLSAHQNADGSWSIAGFSRAG
                     DCECSGDGVYRTKSPGTALAMLPFLGAGQTHLAGKYKDTVSRGLRWLIENQKENGDLR
                     AGSNGNEGMYTHGQAAIVLCEAFAMTGDEELRVPAQKAINFVAAAQYNDGGWRYQPGP
                     RNQQSDTSVVGWQLMALQSARAANLTVPEETWARADLYLDGVQHQNGALYSYQSRSGP
                     TPTMTAEGLLCRMYLGWKNDRPALLAGATYLAEDHLPSRSDPNIYYWYYGTQVLHHLG
                     GPQWDEWNRRMRDVLVISQETRGHAAGSWRADGQHGSAGGRIYMTSLAVCTLEVYYRH
                     LPLYRQLELGK"
     gene            complement(4487..5200)
                     /locus_tag="VFB80_11990"
     CDS             complement(4487..5200)
                     /locus_tag="VFB80_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HZN34537.1"
                     /translation="MMLRILLAASTLIMWAGVAAAQEKPRFIDKGEYVEDVQTGLWWQ
                     KDGQASGKKNFYDAAKYAAGLKLGGRTGWRVPTAKEFETIFPADKAPFTNSAYNPDQC
                     CAGPKEFRSYWTSELDGPPEKDYAFVYHWYAKGGKNNCFASANLVYVRCVLDPKGAAP
                     AAAAPAAEKLITGEEAVQALELIAQLGSDDFARREAAVAGLKAFGRRILPLLTAALEK
                     AQDAEVRFRLKKLIEEAGR"
     gene            5436..5771
                     /locus_tag="VFB80_11995"
     CDS             5436..5771
                     /locus_tag="VFB80_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34538.1"
                     /translation="MTRRSWFCYALGSLAALVARHATAGPEQANLNETLRYVLKARRP
                     QEFAFVDLVTQKVKEGKLPTDLVLSMMKWAVERAREKENPKLPFPYFQEGLRLRAKEL
                     GVDLPPFTP"
     gene            complement(5801..6655)
                     /locus_tag="VFB80_12000"
     CDS             complement(5801..6655)
                     /locus_tag="VFB80_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34539.1"
                     /translation="MTRLIGTLMLLVAVTAAQAHPGVGIVQDSRGNIFFTDLRQVWKV
                     APDGRKSVAVPDVHSHELCLDADDNLYGEHLSGEGGKWRHRVWRLTRDGSLSDVIPAR
                     DGFLRDDSFVRDKAGNRYWTEAGGPAKILKRSTDGRITTLATADLRAIGRMTVTADGT
                     LFLIASGNLHRVTPDGKVMLVAARLSEHSPPPAEVAELNYHMGIWSDAAGNAYVAVAR
                     ERLVLQVRPDGSKKVAARSGDGWSPSGGMFDRDGNLWLLEYDAANAVRIRRTSPPGSE
                     RVILASDP"
     gene            complement(6687..8126)
                     /gene="pyk"
                     /locus_tag="VFB80_12005"
     CDS             complement(6687..8126)
                     /gene="pyk"
                     /locus_tag="VFB80_12005"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZN34540.1"
                     /translation="MPNSALLTSRARTKIVATVGPACNSVDKLSELIRAGVSVFRINT
                     AHGTPQEREQKLADIRAAAAATGFPVGVLVDLAGPKIRLGELAVDPTNCLVGQEFAFV
                     PGAKSNAPHELTSTYPALIGELAVGDSVMLADGTVSMVVIGKTAAEVRCRVVGGGVIR
                     SRQGINLPGAKLSVAALTEADIASAKWAAASEVDFVSLSFVRRAADLVVLKDLLRSHG
                     SAAMTIAKIEKPEALQQLPEIVAAAEGVMVARGDLGVEIDVAEIAVVQKRIVQECQRQ
                     GKPVIVATQMLDSMHHNRRPTRAEATDVANAILDGADACMLSGETAIGDFPVEAVQTM
                     NRISLATEPLIKDQSHIANVQATDVHPITAAAVRGSAIIAEQLGAKLVVVATRGGRTC
                     RLRSKQRDLVPTIGVSDSPSVLRKLSLYWGVTPLAGAPVHDGPQLRAFIDAWGRQHGL
                     IAKGDRILFVTGNNFYPMAQNILVIHEVE"
     gene            8178..8735
                     /locus_tag="VFB80_12010"
     CDS             8178..8735
                     /locus_tag="VFB80_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34541.1"
                     /translation="MAQMEVAGQPRVSALRRFLVWTLIGGAAGVVATLLVVGWMSRDP
                     TPPLTPQIFENAHERWKSAAPQDYDIEVKVSGPQAATYRTEVRRGQPQAAWRNGEPLV
                     GMRTFGTWSVPGMFSTISRDLESIQRRAEGRAMPGAPELILKAQFDSRYSFPQSYRRI
                     EWGSRRGSDAVTVKWDVVEFKVVAP"
     gene            8722..9114
                     /locus_tag="VFB80_12015"
     CDS             8722..9114
                     /locus_tag="VFB80_12015"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HZN34542.1"
                     /translation="MSRPSELENPYAAPASTCIEAADVRCSYCGRPGREVGSLVQSPG
                     GTSYICGGCAKMACELVEYRQRPLVERLAGPIFVIGALGLALCILFWDAFLSADPHVT
                     RFASIPVLMILAVALVVSGLVAVQVLRR"
     gene            9154..9822
                     /locus_tag="VFB80_12020"
     CDS             9154..9822
                     /locus_tag="VFB80_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34543.1"
                     /translation="MRFTLIGLLAFVTAIGVALGCLRTGTTASLDAVNLLFVGSIAFA
                     AAAAWLVRRSQRAYWIGAAAAGWAYWLVISVGLLERTGMDHIDTGLPTDSLVAVLTDV
                     LRRKPDLGDHVWGARPSGLGLPGPDEWSEGTVLETDDPQYLIQWDDGIRPRWTRIRPT
                     AETRTYERSAHSLVGMVLATAGGVLATLACSPRLRRGRPATEWRTTGTRSERKETPLA
                     PSLP"
     gene            complement(9867..>10233)
                     /locus_tag="VFB80_12025"
     CDS             complement(9867..>10233)
                     /locus_tag="VFB80_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34544.1"
                     /translation="VGMYGFALIATIQIVVAWLVKDLWPWRWVFLGYAVAGVYWSIAN
                     AIGAFGDRDTAHQLDSFGARFQWSWQWLILAAIMSLAGFDLYRRRSRDWLHWTGIGYI
                     TAWVVSQSLVILMHRLATS"
BASE COUNT         1636 a         3419 c         3398 g         1780 t
ORIGIN      
        1 gttatttatt cccaataggt tgcgacgaac acgatgcgtc gcggctatgc agtttatgca
       61 acttaagtgc ttcgcctgca tacggaatct cttcggctgt cgcgattcgt ttttgtacac
      121 cactcgcccc cggttgtcaa actgctgtgg cccgcgctgc caatgttaga atcgggcggt
      181 cgaataccgt ggtgactttt cattctcggc gagatgcgtc atgccgattt cgtattcctg
      241 cccgcactgc ggcaagcagt tctcggtcgc cgagcagttc gcgggccaga ccggcccctg
      301 cgccgcgtgc ggcaagccga tctcgattcc cctgtcaggt ggcggttcgg gggccgctgt
      361 ggcggcggcc ggcgtgggaa tcggggcgac actcatgatc gtggcaggcg cggcaatcat
      421 gggattgcta ctctgcgcgg gcctgctggt ggccctgctc ctgccggccg tgcaggcggc
      481 ccgcgaagcc gcgcggcgaa tgcagtcgat gaacaatatg aagcagatca gcatcgcctt
      541 gctcaactac cacgacgtct acggcacctt tccgccggcg gtcgtcaccg acgccagcgg
      601 caagccgctc tacagcggcc gggtgctctt gttgccgttc ctggagcagg atccgatgtt
      661 ccagcagttt cgcaaggacg aggcctggga cagcccgagc aacctgccgc tgacccagca
      721 gtcgctgaaa atcttccagg acccctccgc tccgccgagc gccagcggcc agaccgacta
      781 tctgttcgtc accggcacgg gaaccgctat ggagcccaag cccggtggca tcaagatgaa
      841 cgacatcgtg gacggcacgt cgaacacgat ggccatggtc gagttcaagg gaagcggcat
      901 caattgggcc cagccccgcg acttcgacgt cacgcagccg acagcgctgc cgcccggcaa
      961 tcacccgggc ggcaacctcg tcatgatgta cgacggctcg gtgcggttcg tcagcaagaa
     1021 tgtctcacca cagctgatcc agcagatgtc cacccgcgcc ggcggcgaag tcgtatcgcc
     1081 accgtagcca cattcgttta acccgcagcc ggaggcgacg gcgcgaccgg aaaacaccgt
     1141 cgcctccggc tccgggttaa acaagaattg gtcattaaac ctatggctat tgcctattcc
     1201 tgtccgcatt gcagcaagca gttctcggtc gccgagcagt acgcgggtca gaccggcccc
     1261 tgtgcggcgt gcggcaagcc gatcacgatt cccatggccg gctatgccta cgctcccaag
     1321 gccggcgcgg cggccgcggc cggcggcggc atcgccgtcg tggcgatcgt gctggtggtg
     1381 atgctggtgc tgtgcgcggg cgtgggcctg gccctgctcc tgccggcagt gcaggccgcc
     1441 cgcgaggcgg ccagccggtc gcaatcgtcg aacaacctca agcagatcgg cttggcgctg
     1501 cacaactatc acgacgttta cggcgcgttt ccgcccgccg tcgtcaccga cgcgagcggc
     1561 aagccgctct acagcggccg cgtgctgctg ctgccctacc tggagcagaa caacctctac
     1621 caggccttca acaaggacga gccctgggat agccccaaca accgcattat cagccagacc
     1681 cccatccgca ccttcatgga tccatcgagt ccagaacgac ccgccacagc cggcaagacc
     1741 gactatctgt tcgtcacggg ccaggggacg atcttcgaag cgggcaaaaa cacgaagctg
     1801 atgcagatta ccgacggaac ctccaacacc attctctgca tcgaggtgaa gaacagcggc
     1861 gttaactggg ccgagccgcg cgacttcgac gccagccagc ccgtgccgct gccccccggc
     1921 aaccatccga acgtcaacat cgtgctgttc gccgacggat ccgtgcggac gttgtcgcaa
     1981 tcggccgctt cgccgccgct cgtccggcag atttcgagca aagacggcgg cgaagcggtg
     2041 aacgtgccct actaggccgc tcgctcgctt agccccggcg cccacgccgg ctgcgacgga
     2101 tcaactggcc ggcctggggg ctggggctaa gcggtgaagg actattttcc cagctccagc
     2161 tgccgataca gcggcaggtg ccggtaatag acctccagcg tgcagacggc cagcgaagtc
     2221 atgtaaatcc gcccgccggc gctgccgtgc tggccgtcgg ctcgccagct ccccgccgcg
     2281 tggccgcggg tctcttgcga aatcaccagc acgtcccgca tccgccggtt ccactcgtcc
     2341 cactgcggcc cgcccaggtg gtgcagcacc tgcgtcccgt aataccagta atagatgttc
     2401 gggtcgctgc ggctgggcag gtggtcctcg gccaggtacg tcgcacccgc caagagcgcc
     2461 ggccggtcgt tcttccagcc gagatacatc cggcagagca ggccctcggc ggtcatcgtg
     2521 ggcgtggggc cgctgcgaga ctggtacgaa tagagagcgc cgttctggtg ctgcacgccg
     2581 tcgagataga ggtcggcccg ggcccaggtc tcctccggca ccgtcaggtt cgccgcccgc
     2641 gccgactgca gggccatcag ctgccagccg accacgctgg tgtcgctctg ctggttgcgc
     2701 gggccgggct gataccgcca gccgccgtcg ttgtactgcg ccgcggcgac gaagttgatc
     2761 gccttctggg ccggcacgcg cagctcctcg tcgccggtca tcgcaaaggc ctcgcagagc
     2821 acgatcgccg cctggccgtg cgtgtacatc ccctcgttgc cgttggaccc ggcccgcaag
     2881 tcgccgttct ccttctgatt ctcgatcagc caccgcaggc cgcggctgac ggtgtccttg
     2941 tatttgccgg ccaggtgcgt ctggcccgcg cccaggaagg ggagcatcgc cagcgccgtg
     3001 cccggcgatt tcgtccggta aacgccgtcg cccgagcatt cgcagtcccc cgcccggctg
     3061 aatccggcga tgctccagga gccgtcggcg ttctgatggg cggagagcca gcgcaggccg
     3121 cgggccacgg ccgcctcggt gagcgtcgtg cctccctcct gcttgaccag ctcggtccgc
     3181 agccgcggat cgcgcgaggc gacggcgtgg gtgaagccgt cggcccggcc gatcgcgact
     3241 ttgacggcct gcaggttggg cagattcggc gtgtcgtcgt ccagccgtaa ttcgcgggcg
     3301 tcctgggcgg cggccagcag gacgttccgc tgccggttgt cgttcaggtc cgccttgctg
     3361 ggaagcggca ggtcgaactc ggcccgctcg tcgggcgggt acgcgatggt gtcgcctccc
     3421 tcggtgcgat tgcgtccgta attggtgctc agcaggatgt tccgcccgaa gatcgaggac
     3481 tcttcgtccg taaacagcag ggccagcacg gtgaacgcca ccaggtgcag cagcatgcta
     3541 atgaaccagg ccggcgtgtc gttgagcagg tgtttgagcc agggctcgtg atgcagcact
     3601 cggggcggag ggacgttatt cgtccggccg gcgtggtggg cggagtttct ggcgggcggc
     3661 gcgctagtcg gcgcggccgg ctgagctggg gggctctgtg caggtggttc gggccggggc
     3721 ggacgcggac gcgccggcgc ggccggttct gggcggggcg cgccgcggaa tcgctcggcg
     3781 gccgggcgcg caggcggtgc gagcggctgt tttggcgaat cgttcttggc agcgctcgcg
     3841 cgcggcgctg gcggccgcgg cgcctgcgct ggggcggcgg cttggcgctg ccgctcggcc
     3901 agcagctgct ggacctgccg ctcctgctct tcgctcagct cgatggatgc gccgcaccgc
     3961 caacagtcgg cgagcatcag ccagaaccgc accgacatcg gggcggagca atccggacag
     4021 acgcacagaa tgacgtcgcc gtcgagccag atttcgcccg gtccgcggtt cggcgctggc
     4081 tcggcaggcg agcggggggc gtcagccccc cgatcatcaa ccaccggcag cggccccgcg
     4141 gattccagcc caatcacagg cagtttcgtc tgcggcccgg ttggcagcga gggatcgact
     4201 ccgggcaatt ccagcggcag gaaatcaaac cccacgccgc ccagcccgaa gctgtgctgc
     4261 aattcgctgt ccgcgctggc cgccttggat tctgccgtcg ccatgccgcc ggtcgaaacg
     4321 cgccacgagg tcagccgcgg ctcggcccct gcggcggaac ccggcggcgc cagacgatgg
     4381 gactgggcca gtttcatcct accaccggag tcaaacggca gcgagaacgc gggaaaacgc
     4441 cgtatttgta ttgcgccggc ggcgggggtt gttacaggta gggtgcctac cggccagcct
     4501 cttcgatgag cttcttcagt cggaagcgga cctccgcgtc ctgggctttc tccagggcgg
     4561 cggtcaatag cggcaggatg cggcggccaa aggctttgag gccggccacg gctgcttccc
     4621 gcctggcaaa atcgtcgctg ccgagctgcg cgatcagctc cagggcctgg acggcttcct
     4681 cgccggtgat gagcttctca gctgctggcg cagcggccgc gggggcagca cctttgggat
     4741 cgaggacgca ccgcacgtag acgagatttg cgctcgcgaa gcaattgttc ttaccgccct
     4801 tggcatacca gtgatagacg aaggcgtagt ccttctcggg cggcccatcg agctcgctgg
     4861 tccaatacga gcggaactcc ttggggcccg cacagcattg gtccgggtta taggccgagt
     4921 tcgtgaatgg ggctttgtcg gcggggaaga tcgtctcgaa ttccttggcc gtcggcacgc
     4981 gccagccggt gcggccgccg agcttcaacc ccgcggcgta cttggcggcg tcatagaaat
     5041 tcttcttgcc gctggcctgc ccgtccttct gccaccagag gcccgtctgc acgtcctcaa
     5101 cgtactcgcc cttgtcgatg aaccgcggct tctcctgggc ggccgcaact ccggcccaca
     5161 tgatcagcgt gctcgcggcc agcaggatgc gcagcatcat gttcgtgttc ctctcttcgg
     5221 acagagattg tgctgctgtc atcttcgcag cagcgcccgc cgttgtcgag caatcgctta
     5281 actgcttagc cccggccccc aggccggctg ccgcggaccc ggcgtggacg ccggggctaa
     5341 aaggggggcg gccgctagca ccagattggc gttgccagcc tcacgcggat tttgtatggc
     5401 tcgcggcgaa ttcaattctt ttcaggaccg ccgccatgac gcgccgcagc tggttttgct
     5461 atgcgctcgg aagtctcgcc gcgctggtcg cgcgccacgc cacggccggg ccagagcaag
     5521 cgaatctcaa cgaaacgctg cggtacgtgc tcaaggcccg ccggccgcag gagttcgcct
     5581 tcgtcgatct ggtgacgcag aaagtgaaag agggcaagct gccgacggac ctggtgctga
     5641 gcatgatgaa gtgggccgtc gagcgagccc gcgagaagga gaaccccaag cttcccttcc
     5701 cctacttcca ggaaggcctc cgtttgcgtg ccaaggagct tggcgtcgat ctgccgccgt
     5761 ttacgccgtg aatcggctgt ttgaacatga tccttgacgg tcagggatcg cttgccagga
     5821 tcactcgctc gctgccgggc gggctcgtgc ggcggatgcg caccgcgttg gccgcgtcgt
     5881 attcaagtag ccacagattg ccgtcgcgat cgaacatccc gccggagggc gaccagccgt
     5941 cgccggagcg ggctgcaacc ttcttcgagc catcgggacg cacctgcaag accagccgct
     6001 cccgggccac ggcgacataa gcgttgccgg cggcatccga ccaaattccc atgtgatagt
     6061 tcagctccgc gacttcggcc ggcggcggcg aatgttcgct gagccgggcg gccacgagca
     6121 tgactttgcc gtccggcgtg acgcggtgca aattgccgct ggcgatcagg aacaatgtgc
     6181 catcggcggt caccgtcatc cggccaatcg cgcgcaggtc ggccgtggcg agcgttgtga
     6241 tccggccatc ggtcgagcgc ttgagaatct tcgcagggcc accagcctcg gtccaatagc
     6301 ggttaccggc cttgtcgcgc acaaagctgt catcccgaag gaagccgtcg cgggcgggaa
     6361 tcacgtcgct caagcttccg tcacgcgtca gccgccagac gcggtgccgc catttgccgc
     6421 cctcgcccga caggtgttcg ccgtagagat tgtcgtcggc atcgaggcac agttcgtgcg
     6481 agtgcacatc cggcacagcc acggacttgc ggccatcggg cgcgaccttc cagacctgcc
     6541 tcaggtcggt gaagaaaatg ttgccgcggc tgtcctgcac gatgccgacg ccgggatgcg
     6601 cctgcgccgc agtgacggca accagcaaca tcagtgtccc aatgagcctg gtcacgtcat
     6661 acctcccgac tcctgacccc cgagctctac tccacctcgt ggatcaccag gatgttctgg
     6721 gccatcggat agaagttatt gccggtgacg aagaggatgc ggtcgccttt ggcgatgagg
     6781 ccgtgctggc ggccccaggc gtcgatgaag gcgcggagct gcgggccgtc gtgcaccggt
     6841 gcgccggcca gcggcgtcac gccccagtag agcgacagtt tgcggagcac gctgggcgaa
     6901 tcgctgactc caatcgtggg caccaggtcg cgctgcttgc tccgcagccg gcaggtccgg
     6961 ccgccgcggg tggcgacgac gaccagcttc gcgccgagct gttcggcgat gatcgcgctg
     7021 ccgcggaccg ccgccgccgt gatcgggtgc acgtcggtcg cctgcacgtt ggcgatgtgg
     7081 ctctggtcct tgatcagcgg ctccgtggcc aggctgatgc ggttcatcgt ctgcacagct
     7141 tccacgggaa aatcgccgat ggccgtctcg cccgagagca tgcaggcgtc ggccccgtcg
     7201 aggatcgcgt tggccacgtc ggtggcctcg gcacgcgtcg gccggcgatt gtgatgcatg
     7261 ctgtcgagca tctgcgtggc gacgatcacc ggcttgccct gccgctggca ctcctgcacg
     7321 atccgcttct gcacgacggc gatttccgcc acgtcgatct ccacgcccag gtcgccgcgg
     7381 gccaccatta ccccctcggc ggcggcgacg atctcgggca gctgctgcag ggcctcgggc
     7441 ttttcgatct tggcgatcgt catcgcggcg gagccgtgcg aacggagcag atccttaagg
     7501 acgaccagat cggcggcccg gcggacgaag ctgaggctga cgaaatcgac ctcgctcgcg
     7561 gccgcccatt tggcgctggc gatgtcggcc tcggtcagcg cggccacgct gagtttcgcc
     7621 ccgggcaggt tgatgccctg ccgactgcga atgacgccgc cgccgaccac gcggcagcgg
     7681 acttcggcgg ccgttttgcc gatcaccacc atgctcacgg tgccgtcggc cagcatcacg
     7741 ctgtcgccga cggccaattc gccgatcagc gccggatagg tgctggtcag ctcgtgcggg
     7801 gcgttggatt tcgcgccggg gacgaacgcg aactcctggc cgaccaggca gttcgtcgga
     7861 tcgaccgcca attctccgag gcggattttc ggcccggcca gatcgaccag tacgccgaca
     7921 ggaaagccgg tggccgcggc cgcggcgcgg atgtcggcca gcttctgctc gcgctcctgc
     7981 ggcgtgccgt gggccgtgtt gatgcggaac accgagacgc ccgcgcggat cagctcgctg
     8041 agcttgtcca ccgagttgca ggccggaccg accgtggcca cgattttcgt gcgggcgcgg
     8101 gaggtgagca gggcggaatt cggcatgggc actgttatac ttgagttttc caccgcctgg
     8161 cgattgggga gaagcgcgtg gctcagatgg aagttgccgg ccagccccga gtttccgcgc
     8221 tgcggcgatt cctggtgtgg acgctgattg gcggcgctgc tggagttgtc gccacgctgc
     8281 tggtggtcgg ctggatgtcg cgcgatccga cgccgccgct aacgccgcaa atcttcgaga
     8341 acgcccacga gcgctggaaa tctgccgcac cgcaggacta cgacatcgag gtcaaggtca
     8401 gcgggccgca ggcggcgacc taccgtactg aggttcgccg cgggcagccg caggccgcct
     8461 ggcggaacgg cgagccgctc gtgggaatgc ggacgttcgg cacctggagc gtgcccggca
     8521 tgttcagcac gatcagccgc gacctggaat cgatccagcg gcgggccgaa gggcgggcga
     8581 tgccaggtgc gccggagctg atcctgaagg cccagtttga ctcgcggtac agctttcccc
     8641 agagctatcg ccggatcgaa tggggctcgc gccgcggcag cgacgcggtg accgtgaagt
     8701 gggacgtcgt ggagttcaag gttgtcgcgc cctagcgagc tagagaatcc ctacgccgcg
     8761 ccggcctcga cgtgcatcga ggcggccgac gtccgctgct cgtattgcgg ccgcccgggc
     8821 cgcgaagtcg gctcgctcgt ccagtcgccc ggcggtacga gctacatctg cggcggctgc
     8881 gccaagatgg cctgcgagct ggtcgaatac cgccagcggc cgctggtgga acgcctcgcc
     8941 ggcccgatct tcgtcattgg cgcgctgggt ctggccctct gcatcctgtt ttgggacgcg
     9001 ttcttgtccg cggacccgca cgtcacccgg ttcgccagca tcccagtact gatgatcctc
     9061 gcggtagccc tggtcgtctc cgggctggta gcagtgcaag ttctgcggcg ctagttacca
     9121 tccgccgtcg tatactggcg accgaggcac cgcatgcgct tcaccctgat cgggctcttg
     9181 gccttcgtca ccgcgatcgg cgtcgcgctg ggctgcttgc gcacgggcac gacggcgtcg
     9241 ctggacgccg tgaacctgct gttcgtcggc tcgatcgcct tcgcggctgc cgcggcgtgg
     9301 ctggttcgcc gctcgcagcg ggcctattgg atcggcgcag cggcggcggg ctgggcctat
     9361 tggctggtca tcagcgtggg cctgctggag cggacgggaa tggaccacat cgacactggc
     9421 ttgccgaccg attcgctggt cgccgtgctc accgacgtgc tgcgccgcaa gccggacctg
     9481 ggcgatcacg tgtggggcgc gcggccgagc ggcctgggac tgccgggtcc cgacgagtgg
     9541 tccgagggaa ccgtcctgga aaccgacgac ccacagtacc tgatccagtg ggacgacggc
     9601 atccgcccgc gctggacgcg aattcgcccg accgccgaga cgcggaccta cgagcggagc
     9661 gcgcactcgc tggtgggaat ggtactggcg accgccggcg gcgtgctggc gacgctcgct
     9721 tgtagcccgc gactccgtcg cgggaggccc gccacggagt ggcggactac gggaacgcgc
     9781 agcgagcgta aagaaactcc cctggcccca agcctgcctt gaccggattg tcggcgatgt
     9841 actcgcgaac gtaccgcctt gcgggttcaa gaggtcgcca gccggtgcat caggatcacc
     9901 agactctgtg aaaccaccca agcggtgatg tagccaatac ctgtccaatg caaccagtcg
     9961 cgagagcgcc ggcgataaag atcgaaaccg gccagcgaca tgattgctgc cagaattagc
    10021 cattgccacg accattggaa tcgtgcgccg aaactatcca attggtgtgc cgtatcgcgg
    10081 tcaccgaagg ctccaattgc gttcgcgatc gaccaataca cgcctgctac ggcatacccc
    10141 aggaagaccc atcgccacgg ccataagtct ttgacgagcc aagccacgac aatttgaatg
    10201 gtagcgatga gtgcgaatcc atacatccct acg
//
LOCUS       DASRJB010000455         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2665266, whole genome shotgun sequence.
ACCESSION   DASRJB010000455 DASRJB010000000
VERSION     DASRJB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665266"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFB80_12030"
     CDS             complement(<1..800)
                     /locus_tag="VFB80_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34545.1"
                     /translation="MTSRGKSILTGIVVGSAALASFALIAAQGSRALAQTGAAQAQAA
                     QAREAEAKAREAAAANAKDEAIRAQAAQALPAAPAKGAETVSLDLSKFVLMKADNFVK
                     STRHPWPAVPTGKQTFAGVPVEIQGATMLWGQRNADAGQKYPESVGGIPCGQKFMTLY
                     LLHGTFYEGARGEPSFEVVLNYQGGEKQTDAILCGEDSRDWFAKAGEKMPGPSAKRST
                     LAWTGAGKFNGRDQPIRFCLTAIENKYPDRLVATIDLVSSKKQTAGCIL"
     gene            complement(997..1503)
                     /locus_tag="VFB80_12035"
     CDS             complement(997..1503)
                     /locus_tag="VFB80_12035"
                     /inference="COORDINATES: protein motif:HMM:NF022838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HZN34546.1"
                     /translation="MSRFAALVCAAALCWLVVPGRGQAQEEAVSPEKIPQAVMDALRS
                     KFPQAKIDKCTKAKEGDDVVYDIEFKQAERKCEADIKENGTYINYEKAIAEKDLPKAC
                     RDAIEKKYPKAKLGDIMEETEVTGKTEKLSAYEVTFVTADKKDGEVRLSPDGKILEDT
                     LAAPEAKK"
     gene            complement(1592..2995)
                     /locus_tag="VFB80_12040"
     CDS             complement(1592..2995)
                     /locus_tag="VFB80_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34547.1"
                     /translation="MRTCLLPGLLISLWSAALAAQEPITLSIHPRAIESPALKYRLFP
                     PEADLKSGNAVPILLRLPWEQVNYVSSDKFRDLDDWNSRPLDSPEWKDFRQSFPFRFY
                     DEMKRAAYRREAAWEYPLSEEPPFLILLPDVQGMRQWLGRGLSAKIRYHLSRGELEQA
                     REGILVGLANAKHISTTPFHVNQLVATAIHRIMLDRVGELISQPDSPNLYWALAALPE
                     SLVALDRAADFEGSAFLLTFPAAADLDQPRDAAEWKKMLQQWHELNRYLNRAPHDQIK
                     EAEGTIAPLAKVARGELPGLAGLPADRVAAMSDEEAAVRWLVHVRLSYDQQEAAALSL
                     PPREALPRLKQLRAEKAAIESKVGAARIELRDTTAGYVAMWSLRRRIAALRTIEAVRH
                     HLARYAGKLPANLTDIADVTLPLDPLTDQPFVWKVDGNQATLAAPNLPVDLAPLGPMG
                     QRPGFLEYRLEARPKTD"
     gene            complement(3023..3472)
                     /locus_tag="VFB80_12045"
     CDS             complement(3023..3472)
                     /locus_tag="VFB80_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34548.1"
                     /translation="MNEPRLDPELESLAAKLAAQSPRLPAAESQALLYQCAFAAGQSA
                     ASRNLRRWQGLAAALAVLLAGACLPLAALRLPMAFHRPAASDGEPSPRALADAPADPA
                     EIDVDAWQQPSDSSRFLEQGLTRFGQVDPALRSLAAANLSRRVLDQQ"
     gene            complement(3469..>3696)
                     /locus_tag="VFB80_12050"
     CDS             complement(3469..>3696)
                     /locus_tag="VFB80_12050"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN34549.1"
                     /translation="ATEAADPLELAETLAALERLDADLREVLVARIWGQLSLAEIGQL
                     CGISTATAHRRYESALQTLRLHLAAQCENEP"
BASE COUNT          582 a         1186 c         1253 g          675 t
ORIGIN      
        1 aggatgcagc cggcggtttg cttttttgat gagaccagat cgatggtggc gacgaggcga
       61 tcgggatact tgttttcgat tgcggtcagg cagaagcgga tcggctggtc gcggccattg
      121 aacttgccgg cgccggtcca ggcgagcgtc gagcgcttgg cggacgggcc gggcattttc
      181 tcgcccgctt tggcgaacca gtcgcggctg tcctcgccgc acagaatcgc atcggtctgc
      241 ttctcgccgc cttgatagtt cagcacgacc tcgaacgacg gctcgccccg ggcgccctcg
      301 tagaacgtgc cgtggaggag atagagcgtc atgaacttct gcccgcaggg aatgccgccg
      361 actgactcgg gatacttctg tccggcgtcg gcattgcgct ggccccagag catcgtcgcg
      421 ccttggattt cgaccggcac gcccgcgaag gtttgcttgc cggtgggcac cgcgggccaa
      481 gggtgcctgg tggatttcac aaagttgtcc gctttcatca ggacgaactt cgacaggtcg
      541 agggacaccg tttccgcgcc tttggcgggc gcggcaggta gggcctgggc tgcttgggcc
      601 ctgattgcct cgtccttggc attcgccgcc gctgcctccc gagctttcgc ctccgcttcc
      661 cgggcctgcg ccgcctgagc ttgtgccgcc ccggtctgcg ctagcgcacg cgaaccctgg
      721 gccgcgataa gggcgaagct ggccagggcc gcggatccaa cgacgatgcc ggtcaggatc
      781 gatttgccgc gcgatgtcat gtgagagctc cgtaagaagg atggagagaa gagcagaata
      841 agacattctg tcttaatttg cgccgctcgt ccagccgtgg gtcgcgcaac tgaacccggt
      901 acggtgcgtt cacgtcggtc acaccaactg tgcccaagcc tgtggtcggc tgcgccgcct
      961 acaggcacgc caagttttgt caccttcacc aagcaatcac ttcttcgctt ccggcgccgc
     1021 gagggtgtct tcgaggattt tgccgtcggg agagagccga acctcgccgt ccttcttgtc
     1081 ggccgtcacg aaggtgacct cgtaggcgga gagcttctcg gtcttgcccg tgacctccgt
     1141 ttcctccatg atgtcgccga gcttggcctt gggatacttc ttctcgatgg cgtcacggca
     1201 ggccttgggc aggtccttct cggcgatcgc cttttcgtag ttgatgtacg tgccgttctc
     1261 cttgatgtcg gcctcgcact tgcgctcggc ctgcttgaac tcgatgtcgt agacgacgtc
     1321 gtcgccttcc ttggccttgg tgcacttgtc gatcttggcc tgcgggaact tggaccgcag
     1381 ggcgtccatc acggcctgcg gaatcttctc gggcgatacg gcctcttcct gcgcctggcc
     1441 ccggccggga acaaccagcc agcacagcgc ggcagcacac accagcgcgg cgaacctgct
     1501 catggcgaac tcctgtgaaa tgaggacgag caatccacga gccatcagcg tcggacgtgc
     1561 agccgcaccg gtcaagtaga ggccgggacc gctagtcggt cttgggcctg gcttcgagcc
     1621 ggtattcgag gaagcccgga cgctggccca tcgggcccag cggggccaaa tcaacgggca
     1681 agttgggcgc cgcgagcgtc gcctgattac catccacttt ccacacaaac ggctgatcgg
     1741 tgagcgggtc gaggggcagc gtgacgtcgg cgatatcggt caggttggcc ggcagcttgc
     1801 cggcgtacct ggccaggtgg tgacggacgg cttcgatggt tcgcagggcg gcaatcctcc
     1861 gccgcagcga ccacatcgcg acataaccag cggtggtatc gcggagctcg atgcgggccg
     1921 cgccaacctt gctctcgatc gcggctttct cggcccgcaa ttgcttcagc cgcggcagcg
     1981 cctcgcgcgg cggcagcgag agcgccgccg cttcctgctg gtcgtagctc aggcgcacgt
     2041 gcacgagcca gcgcacggcc gcttcctcat ccgacatggc cgcgacccgg tcggccggca
     2101 gcccggcgag tcccggcagc tcgccgcggg ccaccttggc cagcggcgcg atggtccctt
     2161 cggcttcctt gatctggtcg tgcggcgcgc ggttgagata gcggttcagc tcgtgccact
     2221 gctggagcat cttcttccat tccgcggcgt cgcgcggttg gtccaaatcg gcggcggcgg
     2281 gaaacgtgag caagaaggcg ctcccctcga agtccgccgc gcggtccagc gcgaccagcg
     2341 attcgggcag cgcggcgagg gcccagtaaa ggttcgggct gtccggctgc gagatcagtt
     2401 cgcccacccg gtcgagcatg atgcggtgga tcgcggtcgc gacgagttga ttgacgtgaa
     2461 agggcgtggt ggagatgtgc ttcgcattgg ccaggccgac gaggatccct tcccgtgcct
     2521 gttccagctc gccgcgcgac aggtgatagc gaatcttggc cgacagcccg cggcccagcc
     2581 actgccgcat gccctgtacg tcgggcagca ggatcaaaaa cggcggctcc tcgcttagcg
     2641 gatactccca cgccgcctcg cggcggtagg cggcccgctt catttcgtcg tagaagcgga
     2701 atgggaacga ctgccgaaaa tccttccatt cggggctgtc gagcggccgc gaattccagt
     2761 cgtcgagatc gcgaaacttg tcgctgctga cgtaattcac ctgctcccac ggcagccgca
     2821 gcaggatcgg cacggcgttg ccggacttga ggtcggcttc cggcgggaag agccggtatt
     2881 tgagggccgg cgattcgatc gcccggggat ggatcgaaag cgtgatcggc tcctgggcgg
     2941 ccagcgccgc gctccagagc gaaatcagca gtcccggcag cagacaagtg cgcatggcac
     3001 gactccagtt ctttcgttcg agctattgct gatcgagaac gcgccgcgac aggtttgccg
     3061 cggcgagcga gcggagcgcc ggatcgacct gcccaaagcg ggtcaggcct tgctccagga
     3121 accggctgga atcggacggc tgctgccagg cgtccacgtc gatttcggct gggtcagccg
     3181 gcgcgtccgc cagagcccgc ggggacggct cgccgtccga agcggcgggc cgatgaaagg
     3241 ccatcggcag gcgcagggcc gccagcggca ggcacgcgcc ggcgagcagg accgccagcg
     3301 ccgctgccag gccttgccag cggcgaaggt tgcgagaggc cgcggactgc ccggcggcaa
     3361 acgcgcactg gtagagcagc gcttgcgatt cggcggcggg caatcgcgga gattgcgccg
     3421 cgagtttggc ggcgagcgat tccagttcgg gatcgaggcg aggttcgttc atggttcgtt
     3481 ctcgcattgg gcggccagat gcagccgcaa ggtttgaagg gccgattcgt agcggcggtg
     3541 ggccgtcgcc gtggaaatgc cgcaaagctg gccgatctcg gccagcgaca gctgtcccca
     3601 aatccgggcc acgagcacct cccgcaggtc ggcgtcgagc cgctcgaggg ccgccagcgt
     3661 ctcggccagt tccagcggat cggccgcttc agtggc
//
LOCUS       DASRJB010000456         6125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4075568, whole genome shotgun sequence.
ACCESSION   DASRJB010000456 DASRJB010000000
VERSION     DASRJB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4075568"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFB80_12055"
     CDS             complement(<1..685)
                     /locus_tag="VFB80_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HZN34550.1"
                     /translation="MNSLKMFQNRHSRLWANRKSSRRLFRPLVEPLENRLALAVITVD
                     TTDQEVNSNDKCSLPEAIYAANFDRSLAIDPTATDLQTFISTACMAGDGDDTIVLEEG
                     AVYSMSSVVSDPLNQLGPSATPVIFSDITIRANGATLQRTGDEHLRLFSIADLYTGIN
                     VDGHGISAGTRGELTLHDAFVTGFQTHGGDGRAGGGGGLGAGGAIYVKDSVLYVFNST
                     FAGNSAIGGN"
     gene            complement(759..2894)
                     /locus_tag="VFB80_12060"
     CDS             complement(759..2894)
                     /locus_tag="VFB80_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HZN34551.1"
                     /translation="MSSKRRQLRKTTRSPARRWRPCLEALEQRLALAIGTDLSLADVS
                     VAVTHSPEPPYVGEEVTFLYTIANAGPDAAVNAQFAATLPDVSDFAFVDFGPFPYSGG
                     CSIGGTHFSCHFDADLAPGGSVSASIIMRANTAGSFSHTAVASSDTNDPNSANNQAAE
                     SVAVIPRRPVAIWTGASEVSDLWSDPDNWVGRETPSPDVSEIVITKDIDVPSTKLRST
                     NDFPDGTRFHSITLRGDGLVLQGNAVIVGEGGLISEDGAQTVGIALRTASGLESPNLN
                     LSVGAGSSLTLSGQISGAGGLQKDGPGALILSAANTYGGLTQIDAGTLVVNNNQALGS
                     AASATELLGGTTLMLEGNVTLAESLTLVGVPPGTKLPGVRLVSFGENVINERIATNPL
                     LDISVLGGTLKLGGVLSGDDDLTKAGPGTLVLAADNQLNGTITVQAGSLVVDGSQPNT
                     PIVVEGGSLGGTGLLGPITLAGGQLQGAVLQASPIQPGKRDLVAGGTDGADTILFHPG
                     GTGGSVKVLINGVVQGTFSPDGRLVAYGLGGDDDLQAAGGLQQSAWLDGGAGSDRLKG
                     GSGNDVLQGGVGDDTLQGGSGRDLMIGGSGRDRLIGNDDDDILIGGTTSHDADVAALA
                     AIMAEWTRTDAGFAERAVHLAEGGGHNSLDGGQTFVKLDRHTVHGDDAVDLLTGSAGK
                     DWFFGVWDGGLEDRVTDASLVELLVDLLT"
     gene            complement(3066..>6125)
                     /locus_tag="VFB80_12065"
     CDS             complement(3066..>6125)
                     /locus_tag="VFB80_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN34552.1"
                     /translation="RAEQLQPVKRHVALKIIKPGMDSRQVIARFEAERQALALMDHPN
                     IARVLDGGTVGDAGRPYFVMQLVQGLPVTQFCLERGLSVPERLELFAAICHAVQHAHQ
                     KGVIHRDLKPSNVLVVLESGRPAPKVIDFGIAKAIDQKLTDQTLHTHHGVLVGTLEYM
                     SPEQADLSPAGIDTRSDIYALGVLLYELLTGTTPLDKERLKSADFDEVRRIIREEEPE
                     TVSKRLARTRRVGSAHQSSSAVVSKSHPGGHSPPYRLSELDWIVARCLEKDRNRRYES
                     ASALALDLERYLANEPVSAVPPSAGYRLRKFARRNRGALAVAAGLLLAVTVIAACVGW
                     TVRDRIAQQALQEQAEAARLATVASQVRESLQAARALVAESKVPLAREKLAQAKAQLG
                     NDGTSLASLAAPALAGAAELDRWQHFLDLIDLAHQAETSPPELSLSRSDLSGSSTADS
                     RKAKAAALRALALQSYRILEQPDWKGQLAGGFLEQPQIEQIGRLAYESLLWLANDALG
                     RQKEIPSEASLTPSAAARMAQTYLDRAASSYRPTQALYVLRARCHVVLGDEAAAQSDQ
                     ALAAGTAPSVAVDHFLHGLQAEGGRRLAEAVQAYQQALRLEPNHYWSMMQLGSCLADL
                     GQGPADFREAAGVFTGCILNRPDHAHAYRCRANAWIKLRRLPDALADNTQAIRLDPQS
                     TKAWHNRGVTYRFLGQPDKALADFSKVIELDPRDAKAWNNRGDVFRVLGRLDEAAQNF
                     TRAIELVPQYVLALTNRGLTNAQLGRREEAEADFARALEQDPKSKIAWYGRGSAYLEW
                     KHPDKALADFSQAIELDPRFVDAWNNRGIAHKQLNQPAKALADYSRAVELDPSHAKAW
                     YNRGNTYSLVRQWEHAVADFSKAIELDPKYAAALHYRGVAYLKLSKFEQALPDCKRSV
                     ELAPTERFYWQSLGVAHYRTGDWQAAAGALDMAVELSRGGQATDWLYLAMARQKLGQA
                     DAARQAFARAQEWLDQNKAALAMDEGLADELRQARLEAEEALEMKPK"
BASE COUNT          993 a         2002 c         2009 g         1121 t
ORIGIN      
        1 cgtttccgcc aatcgccgag ttgcccgcga aggtgctatt gaagacgtac agcacgctgt
       61 ctttcacgta gatggccccg cccgcgccga ggccgccgcc gccacccgcg cgaccgtcgc
      121 cgccgtgcgt ttgaaaaccc gtcacgaaag catcgtggag cgtgagctcg cccctcgtgc
      181 cggcggagat accatgccca tccacgttga tgccggtgta gagatcggcg atcgagaata
      241 gccgcaggtg ttcgtcgccg gtgcgctgca gcgtcgcgcc gttggcgcgg atcgtaatgt
      301 ccgagaagat caccggcgtg gcggaagggc cgagctggtt gagcgggtcg ctgacgacgc
      361 tgctcatcga atagaccgcg ccctcttcca gcacgatcgt gtcgtcgccg tcgcccgcca
      421 tgcaagcggt ggaaatgaac gtctgcaagt cggtcgcggt ggggtcgatc gccaggctgc
      481 ggtcgaagtt ggccgcgtag atcgcctctg gcagcgagca tttgtcgttc gagttgactt
      541 cttgatccgt cgtatcgacc gtgatgaccg ccaaggccag gcgattttcc aacggctcca
      601 cgagcggccg aaacaggcgg cggcttgact tgcggttagc ccagaggcgg gaatggcgat
      661 tttggaacat cttgagcgaa ttcatgacat cacttgcagc gagagttaca accagaaaac
      721 cctgaatcgg cgcgcatatg gagcgctggg gtgatagctc aagtcagaag atcgacgagc
      781 agctccacca ggctggcgtc ggtcacgcgg tcctcgaggc cgccgtccca tacgccgaag
      841 aaccagtcct tgccggcgct gccggtcagc aggtccaccg cgtcgtcgcc gtggaccgta
      901 tggcgatcga gcttcacgaa cgtctggccg ccgtccagcg agttgtgacc accgccctcg
      961 gccaggtgca ccgcgcgctc ggcaaaaccc gcgtcggtgc gggtccactc ggccatgatt
     1021 gcggccagcg cggcgacgtc ggcgtcgtga ctcgtcgtgc cgccgatcag gatgtcgtcg
     1081 tcgtcgttgc cgatcagacg atcccgcccc gagccgccga tcatcaggtc tcggccgctg
     1141 ccgccttgca acgtgtcgtc gccaacgccg ccttgcagta cgtcgttgcc cgagccgccc
     1201 ttcagccggt cgctgccagc gcctccgtcg agccaggcgg attgctgaag gccgccggcc
     1261 gcctgcagat cgtcgtcacc acccaacccg taggccacca gtcgcccgtc cggtgaaaac
     1321 gtcccctgca ccacgccgtt gatgagaact ttgacgctgc cgccggtgcc gccgggatgg
     1381 aacaggattg tgtcggcgcc atcggttcca cccgcgacga ggtcgcgttt gcccggctga
     1441 atcgggctgg cctgcagcac ggccccctga agctggccac cggcgagcgt gatcgggccg
     1501 agcagtcccg tgccgccgag cgagccgccc tccacgacga tcggcgtgtt tggctgcgag
     1561 ccgtcgacga ccaggctgcc ggcctggacc gtgattgtgc cgttgagctg gttgtcggcc
     1621 gccagcacca gcgtgccagg gccggccttc gtcagatcgt cgtcgccgct gaggacgccg
     1681 cccaacttca gcgtgccgcc cagcacggaa atgtcgagca gaggattggt cgcgattcgc
     1741 tcattgatca cgttttcacc gaaactgacc aaccgcactc ccggcagctt cgttcccggt
     1801 ggaactccga ccagggtgag actctccgcg agtgtgacgt tgccttcgag catcagcgtc
     1861 gtgccgccga ggagctcggt cgcgctcgcc gccgagccca gagcctggtt gttatttaca
     1921 acgagcgtgc cggcatcgat ctgcgtcagg ccgccgtagg tattggcagc cgagaggatg
     1981 agggcgccgg gtccatcctt ttgcaggccg cccgcgccgc tgatctggcc cgagagagtc
     2041 aagctgctgc cggcgccaac ggagaggttg agattcggag actccaaccc actcgcagtc
     2101 ctcaaggcga tgccgacggt ctgcgcgccg tcctcggaaa tgaggccgcc ttcgccgaca
     2161 atcaccgcgt tgccctgcag gaccaggccg tcgcctcgca gagtgattga gtgaaacctg
     2221 gttccgtcgg ggaagtcgtt cgtgctcctg agcttggtac tcggcacgtc gatgtccttg
     2281 gtaatgacaa tctcggaaac gtccggcgaa ggggtctctc tgccaaccca gttgtccgga
     2341 tcgctccaca gatcgctgac ctcgctggcg ccggtccaga tagccacagg ccggcgaggg
     2401 atcaccgcca ccgactcggc ggcctggttg ttggccgagt tgggatcgtt ggtatccgag
     2461 gaagcgactg ccgtgtggct gaagctgccg gccgtattgg cccgcatgat gatcgaagcc
     2521 gagaccgaac cgccgggcgc caaatcggcg tcgaaatggc agctgaagtg cgtgcctccg
     2581 atgctgcaac cgccgctata tgggaatggg ccaaaatcca caaaggcgaa gtcgctgacg
     2641 tccggcaacg tggcggcgaa ttgggcgttc accgccgcgt ccgggccggc gtttgcgatg
     2701 gtgtagagga acgtcacctc ttcgccgacg tagggcggct cagggctgtg cgtgacggca
     2761 accgaaacat cggccagcga aaggtcggtg ccgatggcca gtgccaggcg ctgttcgagc
     2821 gcctccaggc acggccgcca acggcgagct ggagagcgag tcgttttgcg gagttggcgt
     2881 cgtttgctgc tcatggcgat cggttccgag ttgcgaggta cgaacaaaag aactcagtgc
     2941 cagcgcgcac gttgagcgct ggggcagaac gcttggtcga tggggcggcg aacccgcgga
     3001 gcggatcggc ttggcggcga gaacttaccg gccgcctgat gttgatgggc gacggatgac
     3061 cgtcgttact ttggtttcat ttccagggcc tcctcggcct ccaaacgtgc ctggcgaagc
     3121 tcgtccgcaa gaccctcgtc cattgccagc gcagccttgt tctggtccag ccactcttga
     3181 gcccgtgcaa atgcctggcg ggcggcgtct gcctggccca gcttttgccg ggccatcgcc
     3241 aggtagagcc agtcggtcgc ttgtccgccg cggctcagct ccaccgccat atccaaagcg
     3301 cctgcggcgg cctgccagtc gccggtccgg tagtgggcca cgcccagcga ttgccagtaa
     3361 aagcgttccg tcggggccag ttccacggac cgcttacagt cgggcagcgc ttgctcgaac
     3421 ttgctcagct tgaggtatgc tacgccgcgg tagtgcagcg ccgctgcata cttgggatcc
     3481 aactcgatcg cctttgaaaa atcggcgacg gcgtgttccc actggcggac cagcgaatag
     3541 gtattgcccc ggttgtacca ggccttggcg tggctcgggt ccagctcgac ggcccggcta
     3601 taatcggcga gcgcctttgc cggctggttc agctgcttgt gtgcaattcc ccggttgttc
     3661 caggcgtcga caaatcttgg atccagctca atggcctggg aaaaatcggc cagcgccttg
     3721 tcgggatgct tccattcgag gtaggctgag ccccggccat accaggcgat tttggacttc
     3781 gggtcctgct cgagggcccg agcgaaatcg gcttcggcct cctctcgccg tccaagttgg
     3841 gcattggtca gtccgcggtt ggtgagcgcc agcacatact ggggcaccag ctcgatggcc
     3901 cgcgtgaaat tctgggcggc ctcatccagc cttcctaaaa cgcggaacac gtctccgcgg
     3961 ttgttccagg ccttggcgtc gcgcggatcc agctcgatca ccttggagaa gtcggccaaa
     4021 gccttgtctg gttgtcccag aaagcggtag gtcacgcccc ggttgtgcca ggccttcgtg
     4081 gattgcggat ccagccggat cgcctgcgtg ttgtcggcca aggcgtccgg caagcggcgc
     4141 agctttatcc acgcattggc ccggcagcga taggcgtgcg cgtggtcggg ccggttgagg
     4201 atgcagcccg taaagacccc ggccgcctct cgaaaatccg ccggtccctg cccgagatcg
     4261 gccagacacg aaccgagctg catcatcgac caatagtgat tcggctccag gcgcagcgcc
     4321 tgctgatagg cttggactgc ctccgccagc cgcctgccgc cctcggcctg gagcccgtgc
     4381 aggaagtggt ccacggcaac cgagggcgcg gtcccggcgg ccaacgcctg gtcggactgc
     4441 gcggcggcct cgtcgcctaa gactacgtgg caacgggccc gcagaacata gagcgcctgg
     4501 gtagggcggt acgagctggc ggcccggtcc aaatacgtct gcgccattcg agcagccgcg
     4561 gacggcgtca aactcgcctc cgagggaatc tctttttgcc gccccagcgc gtcgttggcc
     4621 agccagagca ggctttcgta ggccaggcgt ccgatttgtt cgatttgcgg ctgctcgagg
     4681 aagccccccg ccaattggcc cttccaatcc ggctgctcca agattcgata cgattgcagg
     4741 gccagggcgc gcaaggccgc ggctttggcc ttgcgcgagt cggcagtgga cgaaccggag
     4801 aggtccgatc tcgacagcga gagttccggc ggcgatgttt ccgcctgatg ggccaggtcg
     4861 atcaggtcca ggaaatgctg ccagcggtcc agctcggctg ctcccgcaag ggcgggcgcc
     4921 gccaggctgg ccagcgaggt tccgtcattt cccaattgag ccttggcttg ggccagcttt
     4981 tcacgtgcca gcggcacctt gctttccgcg acgagggccc gcgccgcttg cagggattcg
     5041 cgaacctggc tggccaccgt cgccaggcgg gcggcttcgg cttgctcctg gagggcctgc
     5101 tgcgcgatgc ggtcgcggac ggtccagccg acgcaggccg cgatcaccgt cacagccaac
     5161 agcaagccgg ctgcgaccgc cagcgcgccg cggttccgcc gggcgaattt tcgcaggcga
     5221 tatccggctg atggcggcac cgccgacacc ggctcgttgg ccagatagcg ctcgaggtcg
     5281 agcgccagtg cactggccga ttcgtaacgg cgattgcggt ccttctccag gcacctggcg
     5341 acgatccagt caagctcgga gagcctgtag ggtgggctgt gcccaccagg atgcgatttg
     5401 gacacaactg ccgacgagga ttggtgggca gagcccaccc tacgcgttcg cgccagccgc
     5461 ttgctgaccg tctccggctc ttcctcgcgg atgatgcggc ggacttcgtc gaagtcggcg
     5521 cttttcagcc gctccttgtc gaggggcgtc gtgccggtaa gcagctcata gagcaacacg
     5581 ccgagcgcgt agatgtcgct gcgcgtatcg atgcccgccg ggctcaggtc cgcttgctcg
     5641 gggctcatgt actccagcgt gccgaccagc acgccgtggt gcgtgtgcag cgtctggtcg
     5701 gtcagcttct ggtcgatcgc cttggcgatg ccgaagtcga tcaccttcgg cgccggccgg
     5761 cccgattcca gcaccaccag cacgttcgac ggcttgagat cgcggtggat gactcccttc
     5821 tggtgcgcgt gctgcaccgc gtggcagatc gcggcgaaca gctccaggcg ctcgggcaca
     5881 ctcagcccgc gctcgaggca gaattgcgtg accggcaggc cctggacgag ctgcatgacg
     5941 aagtaggggc ggccggcgtc ccctaccgtt cccccatcca gtacccgcgc gatgttcgga
     6001 tggtccatca gtgccagcgc ctgtcgctcg gcttcgaagc gggcgatgac ttgccggctg
     6061 tccatcccgg gcttgatgat cttgagcgcg acgtgccgct tgaccggctg caattgctcg
     6121 gcccg
//
LOCUS       DASRJB010000457         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2665468, whole genome shotgun sequence.
ACCESSION   DASRJB010000457 DASRJB010000000
VERSION     DASRJB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665468"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFB80_12070"
     CDS             <1..201
                     /locus_tag="VFB80_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34553.1"
                     /translation="FPNPRSFETTKNRVVKLAPGQKQTFDVTLEIHGSAQEVAAAEAA
                     VAKLQAGRPPKIFDQPQPDWCA"
     gene            complement(198..956)
                     /locus_tag="VFB80_12075"
     CDS             complement(198..956)
                     /locus_tag="VFB80_12075"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN34554.1"
                     /translation="MRQKPDEIEHALEVFVRGFCAGKSATHPYEHSREGRMWWLRDAP
                     RKNPKNYRKEEWIAYRVPPREADAVARSKTRGRFFVCALRSMDEPEDGLRAGYKELGY
                     RLLATEPLFVHRLTRIPRLEHEVAIERVRTVELAERLGKATRSRPIPAGQLTEDAPFR
                     QYVALEEDNLVGWVRSVNAGDSTWCANMYVRPAHRRRGIGRALLLRMLRDDRRRGAAQ
                     SVLLSSHTGALVYPRVGYEQLGMLLIFAPKRLTN"
     gene            complement(1329..>2015)
                     /locus_tag="VFB80_12080"
     CDS             complement(1329..>2015)
                     /locus_tag="VFB80_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34555.1"
                     /translation="QLMTSSGGQSPWSQNAAAEQWLIHPTQQAPKKICSVVSAAAKPK
                     LDGRLDDPVWQSGKPVALHAARGDDSATGAAVVLAFDDEFLYVAASCRRAAGVDYGPH
                     DSARPAARPSDSDMSPRDHVTLFIDIDRDYGSFWQLAFDDRGWPAESCFGDRHWNPQW
                     YLAAGGDEQFWTVEAAIPLAELTPKRPQVRDVWAVGIQRTIPRVGRQSFTGPAGIESR
                     PETSGLLVFE"
BASE COUNT          343 a          674 c          627 g          371 t
ORIGIN      
        1 tttcccaatc cccgctcgtt cgagaccacc aagaaccgcg tcgtgaagct cgcccccggc
       61 cagaaacaaa ccttcgacgt cacgctcgaa atccacggct ccgctcagga agtcgccgcc
      121 gccgaagccg ccgtcgccaa actgcaggcc ggccgcccgc cgaaaatctt cgatcagccg
      181 caacccgatt ggtgcgccta attggtcagt ctcttcggcg cgaagatcag cagcattccc
      241 agctgctcat atcccactcg cggatagacg agggccccgg tgtggctcga gagcaggacc
      301 gattgcgcgg cgcctcgccg ccggtcgtcg cgcagcatcc gcaggagcag tgcgcggccg
      361 atcccgcggc ggcggtgcgc gggacggacg tacatgttcg cgcaccaggt cgagtcaccc
      421 gcgttgacgc tccgcaccca gccaaccagg ttgtcctcct ccagggccac gtattgccgg
      481 aacggcgcat cctcggtgag ctgcccagcc gggatcggcc gcgaccgcgt ggcctttcct
      541 aatcgctccg ccagctccac ggtccgcacc cgctcaatcg ccacttcatg ctccaagcgc
      601 ggaattcgcg tcagccgatg gacgaacagc ggctccgtgg cgagcaagcg atatcccaat
      661 tccttataac cggcccgcag cccgtcttct ggctcgtcca tgctccgcag ggcacagacg
      721 aagaatcgcc cccgtgtctt gctgcgggcc acggcgtctg cctcgcgggg cggcacgcga
      781 taggcgatcc actcttcctt gcgatagttc ttcgggttct ttcgcggcgc gtcccgcagc
      841 caccacatcc tcccctcgcg cgaatgctcg tacgggtgcg tcgcgctctt ccccgcacaa
      901 aaaccgcgca caaacacttc cagcgcgtgc tcgatctcgt ccggcttttg ccgcatgatg
      961 ggaaattgta gccgggccta tttgcccaac gagggcgaag ctaccgagtt gtgaacattg
     1021 gtatgctatt tgcagtatgg tgcatttttg cgcggtgggc tgccgttttg gcaatggcta
     1081 cgactggcgg ttttccatcg acgtgccgtg ccgtgtatta accgtggcga gtcggccgtg
     1141 gctgaaatct ggatcctcga cctagaccac tggccagggt aaggataatt cgcgccggcc
     1201 ccttctaagt cttataacgc taatctgaag tgatggcctg gcaaatgcgc ctaagtggcg
     1261 ttgccgtctc gacttgggga taagtcgccc ggtttcgcgg gccggaaaac gggacagcta
     1321 atgtggactt actcgaacac cagcaggcca gaagtttctg gtcgcgactc aatgccagcg
     1381 ggtccggtga acgactgccg gcccacgcgg ggaatcgtcc gctggatgcc gacggcccag
     1441 acgtcgcgga cttgcggcct cttgggcgtc agctccgcca ggggaatggc ggcttcgacg
     1501 gtccagaact gctcgtcgcc gccggcggca agataccact gcgggttcca gtggcggtcg
     1561 ccaaagcagc tctccgccgg ccagccccgg tcgtcgaagg cgagttgcca gaagctgccg
     1621 tagtcgcgat cgatatcgat gaagagcgtg acgtggtccc gcggcgacat gtcgctgtcg
     1681 ctggggcggg ccgccggtcg ggcgctgtcg tggggaccat agtcaactcc ggccgcccgg
     1741 cggcagctgg cggcgacgta gaggaactcg tcgtcaaagg cgagcacgac ggcggcgccg
     1801 gtcgccgaat cgtctccgcg agcggcgtgg agcgccaccg gcttgcccga ttgccacact
     1861 gggtcatcga ggcggccgtc gagcttgggt ttggcggcgg cactcaccac ggagcagatc
     1921 ttcttcggag cttgctgcgt cggatgaatc aaccactgct cggcggcggc attttgagac
     1981 cacgggctct gcccgccgct gcttgtcatc aactg
//
LOCUS       DASRJB010000458         5522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2383813, whole genome shotgun sequence.
ACCESSION   DASRJB010000458 DASRJB010000000
VERSION     DASRJB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383813"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFB80_12085"
     CDS             <1..318
                     /locus_tag="VFB80_12085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN34556.1"
                     /translation="FHRVIPDFMVQTGCPQGTGTGGPGYKFADEFHPDLTHSGPGILS
                     MANAGPNTNGSQFFITHVATPHLDGKHSVFGQVIEGQNVVNAIKQGDVMKTVTVAEVP
                     DAK"
     gene            complement(472..1872)
                     /locus_tag="VFB80_12090"
     CDS             complement(472..1872)
                     /locus_tag="VFB80_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN34557.1"
                     /translation="MKTLFPVLCLVICTAVQAADRPPNFVIIYCDDLGYADIGPFGAK
                     GYATPNLDRMAAEGMKLTDFQTAAAVCSASRAALMTGCYPQRVGILGALGPKSQNGIN
                     DSEVLLPEILKARGYATAIFGKWHLGHHPQFLPTRHGFDRYFGLPYSNDMWPNHPTNG
                     QDYPPLPLIEGEKTTLTNPDQTQLTTWYTERAVQFIADSKDRPFFLYLPHNMPHVPLF
                     VSSKFKGKTERGLFGDVIAEIDWSVGQILAALKQHGLDDNTLVLFSSDNGPWLSYGNH
                     AGSAGPHREGKGTVWDGGHCEPTLARWPGKIPPGSVCTELAGTIDVLPTLAKLAGAEP
                     PKDRLIDGRDIWPLLNGQPGAKSPHEAYYYYWNLGLEAVRSGEWKLHLPHEYRTLAGE
                     PGKDGKPGPYKQVKTEQALYNVVADIGESKNVAAEHPDVVARLLKIADTARADLGDSL
                     TGQKGANRRPGGMVAK"
     gene            1981..2529
                     /locus_tag="VFB80_12095"
     CDS             1981..2529
                     /locus_tag="VFB80_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34558.1"
                     /translation="MRSCSLAVVCLIAAPSAAAEVEKLYYVGEAKLSSATGQPMGSQA
                     FLLEKIHDQDNSQIIERAVVVKPDGTANEYPMTMKVKGDSFTITDAENTISGSGKLFG
                     PAWHWTYFKATYSSTNGARIEDENYMADPGVLVARKTVSLPDGKVVMHMDITLKTITP
                     QTFAILAAGLLKKESASKVKQP"
     gene            2556..3086
                     /locus_tag="VFB80_12100"
     CDS             2556..3086
                     /locus_tag="VFB80_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34559.1"
                     /translation="MSFLLFLLLYAGLVFGLIVVHESGHYLAGWLAGIPPRDMRLVLF
                     SFPQHVAIRDGERWLSPVREIERYIEVTKQHLPSRTAAFCWVAGGMVVELATAFALWV
                     AATAAGYGGVAFWVAAISLGMYLINLCFMDLPWAVRYRCAAGDTSGLWQIAPVAAVIF
                     TAVMLASRILLVVLST"
     gene            complement(3093..3623)
                     /locus_tag="VFB80_12105"
     CDS             complement(3093..3623)
                     /locus_tag="VFB80_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34560.1"
                     /translation="MSSFGRGGVLSRFFSGLCEYVFEGQLGVADPPLIDYVSDLMLRF
                     ARLDTAQRVRNRSGRPVVEVADMLSEAENRIGLARRDVHRHIGDVTLFWTGVYPESLQ
                     KLRSVEKKDYFVDYCSQGKRAYYIASTIQTDREEDTPNEILHRLSEQFELCAYGLGEV
                     RREWERRDDDRTLMVD"
     gene            4032..>5522
                     /locus_tag="VFB80_12110"
     CDS             4032..>5522
                     /locus_tag="VFB80_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN34561.1"
                     /translation="MAIRVALHHVTEYRYDRPVTIQPHVVRLRPAPHCRTPILSYSLK
                     VEPKPHFLNWQQDPYSNYLARLVFQQPARGISFTVNLVAEMISINPFDFFIESSAEKC
                     PFAYDAVASRELIPFLEKQDCGPQFDELVRATRQPGERTIDFLVGLNRKLSQLIRYVI
                     RLEPGIQSCGQTLELGSGSCRDSAWLLVQLLRHHGLAARFASGYLIQLTADEKSLDGP
                     SGPEKDFTDLHAWAEVYIPGAGWIGLDPTSGLLAGEGHIPLACAADPVTAAPITGSYS
                     WFDDDKVREDFRHDMSVTRIYEDPRVTKPYTDQQWRRIDSLGREIDEMLRAGDVRLTM
                     GGEPTFVSIDNKDGEEWNTAALGEHKRERAGVLFRRLAGRFAPGGLLHFGQGKWYPGE
                     SLPRWALGCYWRKDGVPIWQNPQLIAEDGSDYRAGPEQAQQFATTLAQKLGVDPKFAI
                     PGHEDAWYYLWRERRLPTNVDPLESQLDDPEERARLAKVFEQGLNTI"
BASE COUNT          963 a         1782 c         1757 g         1020 t
ORIGIN      
        1 ttccaccgcg tcattcccga cttcatggtc cagaccggct gcccgcaggg aaccggcacg
       61 ggcggcccag gctacaaatt cgccgacgaa tttcacccgg acctgaccca cagcggcccg
      121 ggaattctct cgatggcgaa cgccggcccg aacaccaacg gctcgcagtt cttcatcacg
      181 cacgtcgcca cgccccacct cgacggcaag cattccgtct tcggccaggt gatcgaaggc
      241 caaaacgtcg tcaacgccat caagcaggga gacgtgatga agacggtgac ggtcgcggaa
      301 gtgccggacg cgaagtagtc cggtcccccc tctcccattg ggagagtctg acaggcgaag
      361 agaaggatta cgattaggat taagaatagg attaggagca agagaaagat gacgagcatg
      421 acgcgggcag caggatcatg ctcttactct taatcctggc ctccaggagc atcacttcgc
      481 caccattccc cccggccggc ggttggcgcc cttctggccg gtgagcgagt cgcccaggtc
      541 ggcgcgggcg gtgtcggcga tcttgagcag ccgagcaacg acgtccgggt gctcggcggc
      601 gacgttcttc gattcgccga tgtcggccac gacgttgtag agggcctgct cggtcttgac
      661 ctgcttgtag gggccgggct tgccatcctt gccaggctcg cccgcgagcg tgcggtactc
      721 gtgcggcagg tgcagcttcc actcgccgct gcggaccgcc tccaggccca gattccagta
      781 gtaatagtag gcttcgtgcg gcgacttcgc ccccggctgc ccgttgagca acggccagat
      841 gtcgcggccg tcgatgaggc ggtcctttgg cggctccgcc ccggccagct ttgccagcgt
      901 cggcagcacg tcgatcgtgc cggcgagctc ggtgcagacg ctgcccggtg gaatcttgcc
      961 cggccagcgg gccagcgtcg gctcgcagtg gccgccgtcc cagacggtgc ccttgccttc
     1021 gcggtgcgga ccggccgagc ccgcgtggtt gccgtacgag agccacggcc cgttgtccga
     1081 cgagaagagc accagcgtgt tgtcatcgag gccgtgctgc ttgagggccg ccagaatctg
     1141 cccgacgctc caatcgatct cggcaatcac gtcgccgaac aggccccgct cggtcttccc
     1201 cttgaacttg ctggaaacga acagcggcac gtgcggcatg ttgtgcggca gatacagaaa
     1261 gaacggccgg tccttgctgt cggcgatgaa ctgcacggcc cgctcggtgt accaggtcgt
     1321 cagctgcgtc tggtcggggt tggtcagggt cgtcttctcg ccttcgatca gcggcagcgg
     1381 cggatagtcc tggccgttgg tcggatggtt gggccacatg tcgttgctgt agggcaggcc
     1441 gaaatagcgg tcgaagccgt gccgcgtcgg caggaactgc ggatggtgcc ccaggtgcca
     1501 cttgccgaaa atcgcggtcg catagccgcg ggccttcaga atctccggca gcaggacttc
     1561 gctgtcgttg atcccgtttt gcgacttcgg ccccagcgcc cccagaatgc cgacgcgctg
     1621 cggatagcag ccggtcatca gcgcggcccg cgacgcgctg cacacggccg cggccgtttg
     1681 aaaatcggtg agcttcatcc cctccgccgc catccggtcc aggttcggcg tcgcataacc
     1741 cttcgccccg aacggtccga tgtcggcgta gcccaggtcg tcgcagtaga tgatgacaaa
     1801 attcggcggc cggtccgccg cctggacggc ggtgcaaatc acaaggcaga gtaccgggaa
     1861 aagtgtcttc atggtcagcg ttgcctcagg cgggtattct actggtcgct agttcgtgcc
     1921 gcatccgcct cttccgagcc aacttgacaa tcgcattatc actgcgttga aaggaacacc
     1981 atgagatctt gctcgctagc cgtcgtctgc ctgatcgccg cgccgtcggc agcggcggag
     2041 gttgaaaagc tgtactacgt gggcgaggcg aagctcagtt ccgcgactgg acagccgatg
     2101 ggctcgcagg cttttctgtt ggagaagatt cacgaccagg ataatagcca gatcatcgag
     2161 cgcgcggttg tggtcaagcc tgacgggact gccaatgaat atcccatgac catgaaggtg
     2221 aagggcgaca gtttcacgat caccgatgcg gagaacacga tcagcggctc cggaaaactg
     2281 ttcggcccgg cgtggcactg gacgtatttc aaggccacgt actcatcaac caacggcgcc
     2341 cgaattgaag acgaaaacta catggccgat ccgggcgtgc tcgtggctcg caagacggtc
     2401 tccctaccgg acgggaaagt cgtgatgcac atggacatca ctctcaagac catcaccccg
     2461 caaacgttcg ccatcctggc ggcgggactg ctcaagaagg agtctgcgtc aaaggtcaag
     2521 caaccatgac cgtcactggc gcttggcgat gaccaatgag cttccttctg tttctgctac
     2581 tctacgcggg cttggtgttt gggctgattg tggttcatga atcgggacat tacctcgccg
     2641 gctggctggc tggtatcccg ccgcgcgata tgcggctggt gctcttctcc ttcccgcaac
     2701 atgtggcgat tcgcgacggc gagcggtggc tctcgccggt ccgggaaatc gagcgctata
     2761 tcgaggtcac caaacagcat ctgccttcgc gcactgccgc tttttgctgg gtggccggcg
     2821 gaatggttgt cgaactcgcg acggccttcg cgctctgggt cgcagccacg gcagccggct
     2881 acggcggcgt ggccttctgg gtcgctgcca tttcgctggg catgtacctg atcaacctgt
     2941 gcttcatgga cctgccctgg gccgtgcgct atcgctgtgc cgccggcgac acctcgggcc
     3001 tgtggcaaat cgccccggtc gccgccgtga tcttcacggc cgtgatgctc gccagtcgca
     3061 tcctgctcgt cgtgctcagt acctgatcgc gatcaatcga ccatcagcgt ccggtcgtca
     3121 tcccgccgtt cccattcgcg gcggacttcg cccaggccgt aggcgcagag ctcgaattgc
     3181 tcgctgaggc ggtgcaggat ttcgttgggc gtgtcttcct cgcggtcggt ctggatcgtg
     3241 ctggcgatgt agtaggcccg cttgccctgc gagcagtaat cgacgaagta gtccttcttc
     3301 tccaccgacc gcagcttctg cagcgattcg ggatagaccc ccgtccagaa cagcgtcacg
     3361 tcgccgatgt gccggtggac gtcgcggcgg gccaggccga tgcggttctc ggcctcgctg
     3421 agcatgtcgg ccacttcgac cacgggccgg ccggagcggt tccgcacccg ctgcgcggtg
     3481 tccagcctgg caaagcgaag catcaggtcg ctgacgtaat cgatgagcgg cggatcggcc
     3541 acgccgagct gcccttcaaa gacatactcg cagagccccg aaaagaagcg ggacagcact
     3601 ccgccgcggc caaaactgct catggaaaca gcctctccgt ctgcgacttt tcgcgcattt
     3661 gcgacggagg cgacgagcgg cgatcaggga ctattgtccg gcttcgtaaa gcgccaacac
     3721 gtcagccggc agtcgcctat gcgactgaaa ccatccaatt caactaatat ttcgattgcc
     3781 gcagggagtc aaggtcgatg aatgcgggtc ttgcgggctg atccgcctgc cctgcgggcg
     3841 aagaactgcc taacgatcgg gcagccaccc gcgccaagcg gccgcttttc ccgcttttcg
     3901 cggccttgcg ccccacggaa tggcagttgc tccgtacaat cggtgcggct gcgattcccg
     3961 gcggtcgcct tccgccgccg gtcgatattc agcctgatcc cgctcgaatc gcggccttct
     4021 gcccagcttc tatggccatc cgcgttgccc tgcatcacgt tacggaatat cgctacgacc
     4081 ggccggtgac gattcagccg cacgtcgtcc gcctgcggcc ggcgccgcac tgccgcacgc
     4141 cgatcctgag ctactcgctg aaagtcgagc cgaagccgca cttcctgaac tggcagcagg
     4201 atccctacag caactacctg gcccggctgg tcttccagca gccggcccgc ggcatctcgt
     4261 tcaccgtcaa tctggtggcc gagatgatct cgatcaaccc gttcgacttc ttcatcgagt
     4321 cgtccgccga gaagtgcccg ttcgcttacg acgcggtggc cagtcgcgag ctgattccgt
     4381 tcctggagaa gcaggactgc gggccgcagt tcgacgagct ggtccgcgca acgcggcagc
     4441 cgggcgaaag gacgatcgac ttcctggtcg gcctgaaccg caagctcagc cagctgatcc
     4501 gctacgtgat tcgcctggaa ccgggcatac agtcgtgcgg ccagacgctc gagctgggca
     4561 gcggctcgtg ccgcgacagc gcctggctct tggtgcagct attgcggcac catggcctgg
     4621 cggcgcggtt tgcctcgggt tatctgattc agctcacagc cgacgaaaag tcgctcgacg
     4681 ggccgtcggg tccggagaag gacttcaccg acctgcacgc ctgggccgag gtctacattc
     4741 ccggggcggg ctggatcggc ctcgatccga cgagcgggct cttggccggc gaggggcaca
     4801 ttccgctagc ctgcgccgcc gatccggtca cggccgcccc aatcactggc tcgtacagtt
     4861 ggttcgacga cgacaaggtc cgcgaagact tccgccacga catgtcggtg acgcggattt
     4921 acgaagaccc gcgggtcacg aagccttaca ccgaccagca gtggcggcgg atcgattcgc
     4981 tgggccgcga gatcgacgaa atgctccggg ccggcgacgt gcggctgacg atggggggcg
     5041 agccgacgtt cgtgagcatc gacaacaagg acggcgagga atggaatacg gccgccttgg
     5101 gcgagcacaa gcgcgagcgg gccggcgtgc tcttccgccg cctggcgggc cgcttcgcgc
     5161 cgggcgggct gctgcatttc ggccagggca agtggtatcc gggcgaatcg ctgccgcggt
     5221 gggccctggg ctgctactgg cggaaggacg gcgtgccgat ctggcagaat ccgcagctga
     5281 tcgccgagga cggcagcgac tatcgcgccg gtcccgagca ggcgcagcaa ttcgcaacga
     5341 cgctcgccca gaagctcggc gtcgatccca agttcgcgat ccccggccac gaagacgcct
     5401 ggtattacct gtggcgcgag cggcggctgc cgacgaatgt cgatccgctt gagagccagc
     5461 ttgacgatcc agaagagcgg gcgcgtctgg cgaaagtctt cgagcagggt ctgaacacga
     5521 tc
//
LOCUS       DASRJB010000459         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2384102, whole genome shotgun sequence.
ACCESSION   DASRJB010000459 DASRJB010000000
VERSION     DASRJB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384102"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFB80_12115"
     CDS             <1..405
                     /locus_tag="VFB80_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN34562.1"
                     /translation="KRSSPRVPFKGRAQALIFPPLETAAVSDLQDAEIVTSDLSRSGV
                     SLLYRRQLTRGQRLLLLLHDTSRMVEVCWCCRVWDDLFVAGCQFVDVAGPSGAEQLLN
                     AVDAAISEEVVWLDAGSPSSVAAAPVAESRGA"
     gene            554..1786
                     /locus_tag="VFB80_12120"
     CDS             554..1786
                     /locus_tag="VFB80_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN34563.1"
                     /translation="MPSPNMPGPKNPFDLTSAEFIRDPYSAYATLRQGTPVCWDERLS
                     YWLVTRYADVFALLRDHRVSSNSLGDWIGRVPAEQQAAVQPLKEILTNRIVLTDSPDH
                     RRIRGLMQLAFTPRRVELMRREIESYLNHLLGKAEAAGRVELVSQVADPLPAHVIASM
                     LGLPQEDHHRFKQWTDEIYLFMGASAVPLAERARRGTAAAVQLREYLTALFAQIRAKP
                     RDDLLSGMVAAEEQGEKLSQTELFSNVVGMINAAHETTTNLIANTVLHLLQNREQWEA
                     LVADPGLVESTVEEGLRFESPIQVVARRTLEEIEVAGVKIPANQPLALGLAAANRDDA
                     QFPHADRFDIRRPDNKHVAFGGGQHFCLGAALGRLEGQIALATISRRWPKLQLADESI
                     AWRPYPVFRGLKALHLRP"
     gene            1783..2682
                     /locus_tag="VFB80_12125"
     CDS             1783..2682
                     /locus_tag="VFB80_12125"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN34564.1"
                     /translation="MKTFAWTVLLLVLLLGVAGGQDMPLSQVLIDGEGWTLAAAGYQF
                     TEGPAVDQGGIVFFVDVPASKIFKLDLKTKGVTLFAENTGRSSGLAFGGDGRLYACQS
                     GSRAIVAYDAAGKPTEICNGIGVNDLAVTRDNHIFVTDPQGHRVWHVDPSGKKTVVDE
                     GIERPNGVCLWSDQRTLVVADSATESLWTFRIADDHSLEFKQPYSTLQLTTAAIAAGK
                     RTSGADGLKTDAAGRLYVTSQAGLQVFDTQGRLSGVIAKPQEKSLSNVAFAGEKLDTL
                     VVTSADKVYTRRTKTAGWTNFSR"
BASE COUNT          508 a         1044 c          973 g          505 t
ORIGIN      
        1 aagcggtcgt ctccccgggt gccgttcaag ggccgggctc aggccctcat ctttccgccg
       61 cttgaaaccg ctgcggtcag cgatctgcag gacgccgaaa tcgtcaccag cgacctctcg
      121 cgcagcggcg tcagcctgct ctatcgccgg cagctcacgc ggggccagcg gctgctcctg
      181 ctcctgcacg acaccagccg gatggtcgag gtctgctggt gctgccgggt gtgggacgac
      241 ttgttcgtgg ccggctgcca gttcgtcgat gtcgccggcc cctccggcgc cgagcagctg
      301 ctgaacgccg tcgatgcggc cattagcgaa gaggtcgtct ggctcgacgc tggcagccct
      361 tccagtgtgg cggccgcgcc cgtggccgag tcgcgcggtg cctgatattt gacggggcgg
      421 cgcacctggc cttaagctcc acgcagcctt cagcatcgtt taacccggag ccggaggcga
      481 cggcgacacc ggcgaatccg cctgcgcctc gggctgcggg ttaaacaatg atcgccaatc
      541 ggatattccc agtatgccca gtcccaatat gcccggccct aaaaacccgt tcgacctgac
      601 gtcggccgag ttcatccgcg atccctactc ggcgtatgcc acgctgcgcc agggcacgcc
      661 ggtctgctgg gacgagcggc tctcctattg gctcgtcacg cgctacgccg acgtgttcgc
      721 cctgctccgc gatcatcgcg tctcgtccaa ctcgctgggc gactggatcg gccgcgtgcc
      781 cgccgagcag caggcggccg tgcagccgct caaggagatc ctcaccaacc gcatcgtgct
      841 caccgacagc cccgaccacc gccgcatccg cggcctgatg cagctggcgt tcacgccgcg
      901 gcgggtcgag ctgatgcggc gcgagatcga gtcgtatttg aatcacctgc tgggcaaagc
      961 cgaggccgcc ggccgggtcg aactggtttc gcaggtcgcc gatccgctgc cggcgcacgt
     1021 gatcgcgtcg atgctcggcc tgccgcagga agaccatcat cggttcaagc agtggaccga
     1081 cgagatctat ttgttcatgg gggccagcgc cgtgccgctg gccgagcggg cccgccgggg
     1141 gaccgcggcc gccgtgcagc tgcgcgagta tctgacggcc ctgttcgccc agattcgcgc
     1201 caagccgcgc gacgaccttt tgagcggcat ggtcgccgcc gaggagcaag gcgaaaagct
     1261 cagccagacg gagctcttca gcaacgtcgt cggcatgatc aatgccgccc acgagacgac
     1321 gaccaacctg atcgccaaca ccgtgctcca cctgctccag aaccgcgagc agtgggaggc
     1381 cctcgtcgcc gatcccggcc tcgtcgaatc caccgtcgag gagggcctcc gattcgagag
     1441 cccgattcag gtcgtcgccc ggcggacgct ggaggaaatc gaggtcgccg gcgtcaagat
     1501 tcccgccaat cagccgctgg ctctgggcct ggccgccgcc aaccgcgacg acgcccagtt
     1561 cccgcatgcc gaccggttcg acatccgccg ccccgacaac aagcacgtgg ccttcggcgg
     1621 cgggcagcac ttctgcctcg gcgcggctct cggccggttg gaaggccaga tcgccctggc
     1681 caccatcagc cggcgttggc ccaagctgca gctggccgac gaatcgattg cctggcgacc
     1741 ctacccggtc ttccgcggcc tgaaggctct ccacctgcgc ccatgaaaac gttcgcctgg
     1801 actgtactgc tgctggtttt gttgttgggc gtcgctggcg gccaggacat gccgctgtcg
     1861 caggtcctga tcgatggcga aggctggacg ctcgccgccg caggctacca gttcaccgag
     1921 ggcccggccg tcgatcaggg cggcatcgtc ttcttcgtcg atgtcccggc gagcaagatc
     1981 ttcaagctcg atctgaagac caaaggcgtc acgctgtttg ccgagaacac cggacgctcc
     2041 agcggcctgg ccttcggcgg cgatggccgg ctgtacgcct gtcagagcgg cagccgggcg
     2101 atcgtggcct acgacgccgc gggaaagccg acggagattt gcaatggcat cggcgtgaac
     2161 gacctggccg tcaccagaga caaccacatc tttgtcaccg acccgcaagg ccaccgcgtc
     2221 tggcacgtcg atccgtccgg caaaaagacc gtcgtcgatg aaggcatcga acggccgaac
     2281 ggcgtttgcc tgtggtccga tcagcggacg ctcgtcgtgg ccgattccgc caccgagagc
     2341 ctctggacct ttcgcatcgc cgacgaccat tcgctggagt tcaagcagcc ctactcgact
     2401 ctgcagctga ccaccgcggc catcgccgcc ggcaagagga ccagcggcgc cgatggactc
     2461 aagaccgacg ctgccggccg gctgtacgtc acgtcgcaag ccgggctgca agtcttcgac
     2521 acgcagggcc gcctctcggg cgtcatcgcc aaaccccagg agaagtcgct cagcaacgtc
     2581 gccttcgccg gcgaaaagct tgacacgctc gtcgtcacct ccgccgacaa ggtttacacg
     2641 cgccgcacca agaccgccgg ctggaccaac ttctcccgat aagacgcctg gcggtcgcgc
     2701 acccggactt ggccccgctg aaaagcaggc gggacgagta acggcgagcc gttcttgaaa
     2761 agtggtgtac ttggaatttg gccgttggct tgtgcggtgg aaagcgctgt acgatgatca
     2821 aagtaagtgc tctggggctg cggccagggc cctaatgcag gggtgattgc catgcggttt
     2881 gttcttccca ttgcagtcgc agcagcctgt ttaggatgtg gcggaaacgg ttctcagccg
     2941 agcgcgggga atccgcctga cgcggccgcg ccggtggcgc ccaacgggag cggagcggca
     3001 cagacgtctc cgggcgggac actgacgggg
//
LOCUS       DASRJB010000460         9689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4076298, whole genome shotgun sequence.
ACCESSION   DASRJB010000460 DASRJB010000000
VERSION     DASRJB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4076298"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="xylB"
                     /locus_tag="VFB80_12130"
     CDS             <1..1353
                     /gene="xylB"
                     /locus_tag="VFB80_12130"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZN34565.1"
                     /translation="VATIRAVVKKAKLKPADVKAIGLSGQMHGSVFLDKSGKVIRRAL
                     LWNDNRTAAECEEIERRAGGRKKLIQLVANPALTGFTAPKILWLRNKEPKNFEKLAKV
                     LLPKDDIRRRLTGEFATEVSDASGMLLLDVAKRNWSKKLLEKLELDESLLARCHESEE
                     VTGQLTPAVAKQLGLSTDCVVVGGAGDCAANAVGTGVVKRGVLSTSIGTSGVMFVHSD
                     EMQFDPAGRLHTFCHAVHGKWHMMGVSLSAGGSLQWFAEQVCQDAAAKGQSVYDVLNA
                     EAAQVAAGSGGLFFLPYLTGERTPHADPLARGCFIGLTLAHSRGHLARSVMEGVTYAL
                     KDSLTIIREQGVPVNEIRASGGGSKSPLWRQIQADVFGQDVVTINAEQGPAYGVALLA
                     AVGAGAYKNVEEACAATIRVVSRTVTSQSAAKVYDRGFPVYQRLYQSLKEDFRAISEL
                     "
     gene            1658..3013
                     /locus_tag="VFB80_12135"
     CDS             1658..3013
                     /locus_tag="VFB80_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN34566.1"
                     /translation="MSSPPVAPPRACADGVGRVRSVRARKLPAGFLALAASFFLLSPG
                     QSHAQFPIRQIPPRLNVFPAAQPTPLLPPANSLPPQSLPAAPQPSQAPHPAVARIIVP
                     EKGAVSYGSGSLVDVRGQFGLVVTNWHVVRDAAGQISVEFPGGFKSPAQVVKTDHDWD
                     LAALSVYRPPVTPIAITSAAPQLGESLTIAGYGDGKYRAASGSLGNYVSPSPDLPQEM
                     LDIARVEARQGDSGGPIFNQRGELCGVLFGAGHGYTNGSYGGRVLQFLTTVVPGGTPG
                     GDAPAQLALAPPPAEGTPPQATALHQPQPPIGLPLGPPLSKDGWAVRGGAPADAAGSS
                     SPAPMPLDVPLLTPPPPRDEPASRVALAPAALREADAVAAAIHSSLPPRITTGVPASA
                     GVDVNQAPPAALAAAIWRQVGGTTAVDQGKTILAIVGVLSLLVFGWRISRPREPEHDE
                     D"
     gene            complement(3046..4029)
                     /locus_tag="VFB80_12140"
     CDS             complement(3046..4029)
                     /locus_tag="VFB80_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34567.1"
                     /translation="MKIAILHNAVADTDSPSDRDVLVQVAAVEAALRTLGHQSRRLPC
                     TLNFELALESLQADRPELIFNLVESLGGSDRLAHLAGVLIEDLDLPYTGTPAAALHLT
                     NNKPLTKARLREAGLPTADWAVTDGPPAALDPPYIIKAIWEHASLGLDDHAVITAGDG
                     ALVQGQIASRSRQLGQLCFAERYIDGREFNLSLIATGNGPCVLPPAEIDFSKFPAGKP
                     KIVGYAAKWNEAAFEFENTPRKFDHAAADTPLVDWLRALAVECWDLFGLRGYARVDFR
                     VDRQGQPWILELNANPCLSPDAGFAAALAQANVPLERAIAWIIDDALRPAR"
     gene            complement(4026..5045)
                     /locus_tag="VFB80_12145"
     CDS             complement(4026..5045)
                     /locus_tag="VFB80_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZN34568.1"
                     /translation="MRIGLTYDLRQDYLDAGFSEDETAEFDRISTVEAIEASLGQLGH
                     QSERIGHARQLARRLVSGETWDLVFNIAEGLSGASREAQIPSLLDVYGIAYTFSDPLV
                     MALTLHKGLTKTVVAAGGVPTPRFAVVEKPADVEPIDLPYPLFAKPVAEGTGKGVSPA
                     SKVKDRAALQAVCDRLLAEHQQPVLVETYLPGREFTVGITGSGASAVAVGTVEILLLE
                     GAEADAYSYLNKERCEQLVEYRLVHAGRDEQVRLAEKVALAAWRILGCRDAGRVDLRC
                     DAAGQPSFMEVNPLAGLHPEHSDLPILATKAGLPYVELIGRIVASAAPRVEEARHSKA
                     RRFRA"
     gene            5181..6203
                     /locus_tag="VFB80_12150"
     CDS             5181..6203
                     /locus_tag="VFB80_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN34569.1"
                     /translation="MTRAIRFVVAVLAYVVLAPGADRLHAQAAISAANADKVSAATEL
                     PVRAHKILRGPGRGELTFLDWNDSVQVVDDVHFRPLRKLAEGRKPIDFTISRDGQHFL
                     WNERGKTAYIVEEAATGTTLEIEIGKTPGFAAFSPDGKLLAIGSSFWDPLIEGAGFSE
                     MQLFDRSGKLVRKLEKSGAGALMPVFSSDGKLLAVGNRNHQTQVFEVDSGKLLHTLDR
                     KMTQEIAFSPDNTTLAAGYVDGMVVVWDMATGQPQRAAVSGCDEIYSVDWSPKGDVLV
                     TSGRKGKIVLWDKDLARLKELDAPLWVIQARFTEDGARLLTSSASDLTARTDRKVVVW
                     TVPAEK"
     gene            6337..7668
                     /locus_tag="VFB80_12155"
     CDS             6337..7668
                     /locus_tag="VFB80_12155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZN34570.1"
                     /translation="MFKMFSLGDSARQLSAARTVLTQLAEVLDAGVSVQLWDGSQVPL
                     GHSADGRLVIEIRGPGVIGSLLRWPTLDNLLRHYATGNIEVHGGDMIELFQALRVEKS
                     RQRLKKLSLGLLLKSAVPFLFSRADEARPQHAYAGNHLARASAARDNRDYIQFHYDLG
                     NDFYELFLDPQMQYSCGYFSDWNNSLEQAQHDKLEMICRKLRLQPGERLLDIGCGWGG
                     LVCYAAQKYGVEAHGVTLSQPQCEYAQARIRRLGLENRVTVELRDYTTLAGKFDKIAS
                     VGMYEHVGIANYPAYFGKIQSLLRDRGILLNHGITRPAKRTKRGFHRVRPEHRLILKY
                     IFPGGELDHIGHTCESLEASRFEVHDVEDWREHYAQTTRLWCQRLSARRDEAIAMVGP
                     ERYRLWVAYLAGVSFAFLDGSVRIYQVVASKHAARGRSELPLTRADLYRAA"
     gene            complement(7675..7757)
                     /locus_tag="VFB80_12160"
     tRNA            complement(7675..7757)
                     /locus_tag="VFB80_12160"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7721..7723),aa:Tyr,seq:gta)
     gene            8603..9433
                     /locus_tag="VFB80_12165"
     CDS             8603..9433
                     /locus_tag="VFB80_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34571.1"
                     /translation="MRNLALGLALCVLAWSGTASGQLPKYNPYAPTQDDPLPVGQDGK
                     LNWPAFFKDKAKEDRFQGYFKIGACVGTREEINTMLKNNKVNVNALPEAAVQGQAASL
                     GSGTVIIADAAGKKTLVVTHPAGVSKVSVSGPMTIRQLKPEMIVRFIGRVDAHGMGVD
                     PVDSLEVVTPDANFRWPQIEADKLRTVTAKVVKLTANRIQVQLPAGKLRKLTFTLADE
                     PKVNVDSSSLALIGAGDAVSAKGRTYTGPGAGGIQVVFASDVTITKALAASANSASER
                     "
     gene            9600..>9689
                     /locus_tag="VFB80_12170"
     CDS             9600..>9689
                     /locus_tag="VFB80_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN34572.1"
                     /translation="MVYSDSPRGSRRAFTLVELLVVIAIIGVLV"
BASE COUNT         1661 a         3162 c         3255 g         1611 t
ORIGIN      
        1 gtcgccacga tccgggccgt cgtgaagaag gcgaagctca agccggccga cgtcaaggcg
       61 atcggcctgt cggggcagat gcacggctcg gtgttcctcg ataagagcgg caaggtcatc
      121 cgccgggcac tgctctggaa cgacaaccgc acggcggccg agtgtgagga gatcgagcgg
      181 cgggccggcg ggcgaaagaa gctgattcaa ttggtcgcca acccggcgct caccggtttt
      241 accgccccga aaatcctctg gctgcggaac aaggagccca agaacttcga gaagctcgcc
      301 aaggtgctgc tgcccaagga cgacatccgc cgccgcctga ccggcgaatt cgccacggaa
      361 gtgagcgacg ccagcggcat gctgctgctg gacgtagcca agcgcaactg gtcgaagaag
      421 ctgctggaaa agctggagct cgacgagtcg ctgctggcca ggtgccacga gtcggaggaa
      481 gtgacgggcc agctcacgcc cgctgtggcc aaacagctcg gcctctctac cgactgcgtc
      541 gtggtcggcg gcgcgggcga ttgtgccgcc aatgccgtcg ggacgggcgt cgtcaagcgc
      601 ggcgtactct cgacgtccat cggcaccagc ggcgtgatgt tcgtccactc cgacgagatg
      661 cagttcgatc ccgccggccg gctgcacacg ttttgccacg cggtgcacgg caagtggcac
      721 atgatgggcg tcagcctgag cgccggcggc tcgctgcagt ggttcgcgga gcaagtctgc
      781 caggatgccg cggccaaggg ccagagcgtc tatgacgtgc tcaacgccga ggcggcgcag
      841 gtcgccgccg gcagcggggg gctgttcttc ctgccttatt tgactggcga acgcacgccg
      901 cacgccgatc cgctggcccg cggctgcttc atcggtctga cgctggccca cagccgcggc
      961 cacctggccc gcagcgtgat ggagggcgtg acctacgccc tcaaggacag cctgacgatc
     1021 attcgcgagc agggcgtgcc ggtcaacgag atccgcgcgt cgggtggcgg cagcaaaagc
     1081 ccgctctggc ggcagattca ggccgacgtg tttggccagg acgtggtgac gatcaacgcc
     1141 gagcagggcc cggcgtacgg cgtcgcgctg ctcgccgccg tcggcgcggg cgcgtacaaa
     1201 aatgtcgaag aagcttgcgc cgctacgatc cgcgtcgtca gccgcaccgt tacgtcccag
     1261 agcgccgcca aggtttacga ccggggattt cccgtttacc agcggctgta tcagtcgctg
     1321 aaggaggact tccgggcgat cagcgagctg taaaacgctt agccccggcc cccaggccgg
     1381 ctggccgtgg accggcgtgg acgccggggc taagcgggat aacgaagtca acaatctttg
     1441 ccggtccaga taagcgcagc gttccagcgg cgcggctaat gcggcaaatc gcggcaagcc
     1501 gcaatcaagt cgagcgggtg gcgggccgcg ggcgatgaag agaaggcacg accgcgatct
     1561 cggcggctac ctccgagcgc ctcggctcct ccgaagccgg gtgcgcggcc gccggacgcg
     1621 cgtgtgtcac ctcgcgtggc ggaaatcgag gtgcgaagtg tccagccccc cggtcgcccc
     1681 tccccgggct tgcgccgacg gcgtcggccg tgtgcggagt gtccgcgcgc gcaagctgcc
     1741 ggcgggattt ctggccctcg ctgcctcgtt tttcctgctt tcgccgggcc agtcgcacgc
     1801 gcagtttccg atccggcaga ttccgccgcg gctgaacgtg tttcctgcgg cgcaacccac
     1861 gccgcttttg ccgccggcga attcgctgcc gccgcaatcg cttcccgcag cgcctcagcc
     1921 gtcacaagcg ccccatccgg ccgtggcgcg aatcatcgtg ccggagaagg gcgcggtctc
     1981 ctacggcagc gggtcgctcg tcgatgtccg cgggcaattc gggctggtcg tgacgaactg
     2041 gcacgtcgtc cgcgatgccg cggggcaaat cagcgtcgaa ttccccggcg gattcaagtc
     2101 gccggcccag gtcgtgaaga ccgatcacga ctgggacctg gcggcgctca gcgtctaccg
     2161 gccgcccgtg acgccgatcg ccattacctc cgcggccccg cagctcggcg aatcgctgac
     2221 gatcgccggc tacggcgacg gcaagtatcg cgccgcctcg ggctcgctgg gcaactacgt
     2281 ctcgcccagc cccgacctgc cgcaggagat gctcgacatc gcccgcgtcg aggcgcgaca
     2341 aggtgattcg ggcggaccga tcttcaacca gcgcggcgag ctgtgcggcg tgttgttcgg
     2401 cgcggggcac ggctacacca acggcagtta tggcggccgc gtgctgcagt tcctgacgac
     2461 ggtcgttccc ggcgggacac cgggcggcga cgcgcccgcg cagcttgcac ttgctccacc
     2521 gccggccgag ggaacgccgc ctcaagcgac ggcgctgcat cagccgcagc cgccgatcgg
     2581 attgccgctt gggccgccgc tttcgaaaga cggctgggcg gtgcgcggcg gtgcgcctgc
     2641 cgatgcggcg ggatccagct cacccgcgcc catgccgctg gacgttccgc tgctgacgcc
     2701 gccgcctccg cgcgatgaac cggcctcacg cgtggccctc gctcccgccg cgctgcgcga
     2761 agcggacgca gtcgccgccg cgattcacag ctcgctgccg ccgcggatca ctactggtgt
     2821 gcccgccagc gccggtgtgg acgtcaatca agcgccgccc gcggcgctgg cggctgccat
     2881 ctggcggcaa gtcggcggca ccaccgcggt cgatcagggc aagacgatcc tggcgatcgt
     2941 cggcgtgctg agcctgctcg tcttcggctg gcggatcagc cgtccgcgcg agccggaaca
     3001 tgacgaggac tagcgcaagt gcggaatgcg gaatgaggga aatagtcatc gagccggccg
     3061 tagcgcgtcg tcgatgatcc aggcgatcgc ccgttccagc gggacgttag cctgggccag
     3121 cgcggcggcg aacccggcgt cgggcgagag acagggattg gcgttgagct cgagaatcca
     3181 gggctgcccc tggcgatcga cgcgaaaatc gacgcgggcg tagccgcgga ggccgaacag
     3241 gtcccagcac tcgaccgcca gcgcccggag ccaatcgacc agcggcgtgt cggcggcagc
     3301 gtggtcgaac ttccgcggcg tgttctcgaa ctcgaacgcg gcctcgttcc acttggcggc
     3361 gtagccgacg attttgggct tgcccgccgg gaacttggaa aagtcgatct ccgccggcgg
     3421 caagacgcac gggccgttac ccgtggcgat cagcgagaga ttgaactcgc ggccgtcaat
     3481 gtaccgctcg gcaaaacaca gctggccgag ttgccgcgag cgcgaggcga tttgcccctg
     3541 gaccagcgcc ccgtcgccgg cggtgatgac ggcgtgatcg tcgaggccga gtgacgcgtg
     3601 ctcccagatc gccttgatga tgtagggcgg atcgagggcc gccggcgggc catcggtcac
     3661 ggcccagtcg gccgtcggca aacccgcctc gcgcaggcgg gccttcgtca gcggcttgtt
     3721 gttggtgagg tgcaacgcgg ccgcgggcgt gcccgtgtac ggcaggtcga gatcctcgat
     3781 cagcacgccc gccaggtgcg ccagccggtc ggacccgccc agcgattcga cgaggttgaa
     3841 gatcagctcc gggcggtccg cctgcagcga ttccagggcc agctcgaagt tgagtgtgca
     3901 gggcagtcgc cgcgactggt ggcccagcgt gcggagcgcc gcctcgaccg cggccacttg
     3961 cacgagcaca tcgcggtccg acggcgaatc ggtgtcggcg acggcgttgt gaaggatggc
     4021 gattttcaag cgcgaaatct ccgagccttc gagtgcctcg cttcctcgac gcgcggggcg
     4081 gcgctggcga cgatccgccc gatcagctcg acgtacggca ggccggcctt cgtcgccagg
     4141 atcggcaaat cggagtgttc gggatgcaga ccggccagcg gattgacctc catgaaactc
     4201 ggctgcccgg cggcgtcaca ccgcagatcg acccggccgg cgtcgcggca gccgaggatc
     4261 cgccaggccg ccagcgcgac cttttcggcg aggcggacct gctcgtcgcg gccggcgtgc
     4321 accaggcggt actcgaccag ctgctcgcag cgctccttgt tgaggtacga ataggcgtcg
     4381 gcctcggcgc cttccagcag caggatctcg accgtaccga cggcgaccgc gctggcgccg
     4441 ctgccagtga tgccgacggt gaactcgcgg cccggcaagt aggtctcgac gagcactggc
     4501 tgctggtgct cggcgagcag gcgatcgcag acagcttgca aggcggctcg gtccttcact
     4561 ttggacgcgg gcgacacgcc cttgccggtg ccttcggcca ccggcttggc gaagagcgga
     4621 tagggcaagt cgatgggttc gacgtcggcg ggcttctcga cgaccgcaaa tcgcggcgtc
     4681 ggcactccgc cggcggccac gacggtcttg gtcagcccct tgtgcagggt gagcgccatc
     4741 acgagcggat cggagaacgt gtaggcaatg ccatacacat cgagcagcga cggaatctgc
     4801 gcctcgcggc tggcgccaga cagcccctct gcgatgttga acaccaggtc ccaggtctcg
     4861 ccacttacca gccgccgggc cagctgccgg gcgtggccga tccgctcgga ctggtggcca
     4921 agctgtccga gggacgcttc gatcgcttcg acggtcgaaa tgcggtcgaa ctcggccgtt
     4981 tcgtcctcgc tgaatccggc atcgaggtag tcctgccgca ggtcataggt caatccgatg
     5041 cgcatcgcgt cacactagcc cccaggctgc cgtcgcggaa ggccgaacat cgcttgcgcc
     5101 gctggcaggt tccaattttt cccgctggtg cgagcttcgt tctccggtct agactgtcgg
     5161 ctccactacg gaggccaggc atgacccgcg caatccgttt cgtcgtcgcc gtgctggcgt
     5221 acgtggtgct cgcgcctggc gcggacaggc tgcacgctca ggctgccatc tccgcggcca
     5281 atgccgacaa ggtcagcgcc gctacggagc tgccggtgcg ggcgcacaag atcctgcgcg
     5341 gcccgggacg cggcgaattg acgttccttg attggaacga ctccgtgcaa gtcgtggacg
     5401 acgtgcattt tcgcccgctg cggaagctcg ccgagggccg caagccgatc gacttcacca
     5461 tcagccgcga tggccagcac ttcctgtgga acgagcgcgg caagacggcc tacatcgtgg
     5521 aggaagctgc caccggaacg acattggaga tcgagatcgg caagacgccc ggcttcgccg
     5581 cgttcagtcc cgacggcaag ctgctggcga tcggcagcag cttttgggat cctcttatcg
     5641 aaggcgccgg cttctctgag atgcagctct tcgaccgctc gggcaagctc gttcggaagt
     5701 tagagaaatc gggcgccggt gcgctgatgc ccgtcttcag ctcggacggc aagctgctgg
     5761 ccgtcggcaa ccgcaaccac cagacgcagg tcttcgaagt ggactcgggc aagctgctcc
     5821 acaccttgga tcgcaagatg acccaggaga tcgccttcag ccccgacaac acgaccctcg
     5881 ccgccggcta cgtagacggg atggtcgtcg tctgggacat ggccaccggt cagccgcagc
     5941 gggcggcggt ctcgggctgc gacgaaatct actccgtcga ttggagtccg aaaggcgacg
     6001 tgctggtcac gtctggccgc aagggcaaga tcgtcctgtg ggacaaggac ctggcgcgct
     6061 tgaaggagct cgatgccccg ctgtgggtca ttcaggcccg ctttaccgag gacggcgcac
     6121 gcctgctcac ctctagcgcc tccgacctca cggcccggac tgaccgcaag gtcgtcgtct
     6181 ggaccgtccc ggctgagaaa tagcagcggc ttccaaccgc agcggtcaat cttgcggtga
     6241 ttgccctgct ttgctaaatc cccacaccgt cgggagcgtt ccgatccctc ggcggctgga
     6301 agtcgccgcg cgaagttgtg acggctggag gctcgtatgt tcaagatgtt ctcgctgggc
     6361 gattcggcgc ggcagctgtc ggcggcgcgg acggtgctga cgcaactggc ggaagtgctc
     6421 gatgccggcg tcagcgtgca actctgggat ggctcgcagg tccccttggg gcacagcgcc
     6481 gacggccgtc tggtcattga aatccgcggg ccgggcgtga ttggctcgct gctccgctgg
     6541 ccgacgctcg acaacctgct gcggcactac gccacgggaa acatcgaggt ccacggcggc
     6601 gacatgatcg agctgttcca ggcgctccgc gtcgagaaat cgcgccagcg cttgaaaaag
     6661 ctcagcctgg gcctgctgct caaaagcgcg gtgccgttcc tcttctcccg ggcagacgag
     6721 gcccggccgc agcacgctta tgcgggcaac cacctggccc gcgcctcggc cgcgcgggac
     6781 aaccgcgact atatccagtt ccactacgac ctgggcaacg acttttacga gctgtttctc
     6841 gatccgcaga tgcagtactc ctgcggctac ttcagcgact ggaacaactc attggaacag
     6901 gcccagcacg acaagctgga gatgatctgc cgcaagctcc gcctgcagcc gggcgagcgg
     6961 ctgctcgaca tcggctgcgg ctggggcggt ctggtctgct acgcggcgca aaagtacgga
     7021 gtcgaagccc acggcgtgac gctctcgcag ccgcagtgcg aatatgccca ggccaggatt
     7081 cgccggctgg gtctggaaaa ccgggtcacg gtggagctgc gggactatac gacactggcg
     7141 ggcaagttcg acaagatcgc cagcgtcggc atgtacgagc acgtggggat cgccaactat
     7201 ccggcctatt tcggcaagat ccagtcgctg ctccgggacc gcggtatcct gctgaatcac
     7261 ggcatcacgc ggccggccaa gcgaacgaag cgcggctttc accgcgtccg gcccgagcac
     7321 cgcctgattt tgaaatacat cttccccggc ggcgaattgg accacatcgg tcacacctgc
     7381 gagtcgctcg aagcgagccg ctttgaggtc cacgacgtgg aggactggcg cgagcattat
     7441 gcccagacga cgcggctctg gtgccagcga ctgagcgccc gccgcgacga ggcgattgcg
     7501 atggtcggcc ccgagcgcta ccggctgtgg gtggcctatc tggcgggagt ctccttcgcg
     7561 tttctcgacg gctcggtccg catttaccag gtcgtggcca gcaagcacgc cgcccgcggc
     7621 cgctccgaat tgccgctaac gcgggcggac ttgtaccgcg cggcgtgaag taagtggaca
     7681 gagcaggatt cgaacctgcg taggcaaatg ccacctgttt tacagacagg tccctttaac
     7741 cgctcgggca tctgtccagt ccaaacgacg gacacggctt tcgccggtcc ggttcgtgtt
     7801 tggttggttc accgggcgaa ttgcccggct gtctatccaa gcggggaaac tggggttctg
     7861 tttcagctac gatcaggcgg aaacaaagta ggccccttct gccggaaggg gccttcaggg
     7921 aagcgtagcg accaggaacg ttgccgagat tcgaccgcac cgcgctgcca ggtcccgccc
     7981 ggcaggcggg acctacgacc agcattggag tggcacctat gaaaaaatcg aaggcgctga
     8041 gagagccatt aaggctctcc gacgaagttg aaaattcttc gcgccagcac ccctccaatg
     8101 agggttcgag gcgtgggccg tttgctataa tctgttcact acggaaaatt gacttcgccc
     8161 ggtgagcggg cggccggatg ttctttggaa attcgtggca ggcgaccgac gcgtagccgg
     8221 tcgccagcgt tggcgtgggt gctgagtgca tcaggcgcag gttgcgatac gaggcgcggt
     8281 tgagcagcga atttttcgcc acccgcattt cgttcgtaac tgccgacgcg acaaggcact
     8341 tgcggccggc ggcggggaat tgatcgccag acccccagat atcgaggcag attggcgaac
     8401 aattcgcggc ccaaagtgcg gaaaagctag cgattttcgc aaaacgcggc cgaattattc
     8461 gccaccgagc tgcccgaccc ggcgaagaac gttcgaccgc cactcgcgct tccgacgggc
     8521 ttcggacttg actgcgcagc tttcccagtt taacacggaa tccggagcag gcgtccgaga
     8581 ccaaactttt acggggtgtg ttgtgcgaaa tctggcgctg ggattggctt tgtgcgtgct
     8641 cgcttggagt ggcactgcga gcgggcagct tcccaagtac aacccgtacg cccccacgca
     8701 ggacgatccg ctgccggtgg gtcaggatgg caagctcaac tggccggcgt ttttcaagga
     8761 caaggcgaag gaggaccgct ttcagggcta cttcaagatc ggcgcctgcg tcggcacgcg
     8821 ggaagagatc aacacgatgc tgaagaacaa caaggtcaac gtgaacgcgc tgccggaggc
     8881 ggcggtgcag gggcaagctg ccagcctcgg cagcgggacc gtgatcatcg ccgacgcggc
     8941 ggggaaaaag acgctggtcg tgacgcatcc ggccggcgtg agcaaggtca gcgtcagcgg
     9001 gccgatgacg atccggcaac tcaagccgga gatgatcgtg cggtttatag gccgcgtcga
     9061 tgcgcacggg atgggtgtcg atcccgtgga cagcctggag gtcgtcacgc ccgatgccaa
     9121 tttccgctgg ccccagatcg aagccgacaa gctcaggacg gtgacggcga aggtcgtcaa
     9181 actcaccgcc aaccggattc aggttcagct tcccgccggg aagcttcgca agctgacgtt
     9241 tacgctggct gatgaaccga aggtcaacgt cgattccagc agcctggccc tgatcggcgc
     9301 tggcgacgcc gtttcggcca aaggccgcac ctacaccggc cccggcgctg gcggcataca
     9361 agtcgtcttc gcctccgacg tgaccatcac caaggcgctg gctgcctcgg cgaattctgc
     9421 ctcggaacga taggacgggc ggtgtgcctg cggccggagc cggcctggtc acacctgacg
     9481 tccacttcgc ggattgttaa gcttgcgtta tcgcctgcgc gcgggataat gctggcgcta
     9541 cgaaacagga agagtgcggc gctcagcgtc tgttcacggt tcgttccgga gtcttgctca
     9601 tggtctattc agacagcccg cgcggctctc gtcgcgcctt cacgctcgtc gagctgctcg
     9661 tcgtcatcgc catcatcggc gtcctcgtg
//
LOCUS       DASRJB010000461         5360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_128181, whole genome shotgun sequence.
ACCESSION   DASRJB010000461 DASRJB010000000
VERSION     DASRJB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128181"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..285)
                     /locus_tag="VFB80_12175"
     CDS             complement(34..285)
                     /locus_tag="VFB80_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34573.1"
                     /translation="MATASELDDWRRSRRQSLRALPGHAVFVLALTAVLLALHWLLGL
                     WISACLAILALGSFSLAGDLINIIWVSRRIAQAERRQRL"
     gene            complement(338..3937)
                     /gene="secD"
                     /locus_tag="VFB80_12180"
     CDS             complement(338..3937)
                     /gene="secD"
                     /locus_tag="VFB80_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZN34574.1"
                     /translation="MNVFHWLQSLSPILAENRYRLIDFDWPPILPDSLNVASFPAVLL
                     GLIVVSFVLGWLFERATRLKDYGWKVGLILATTLVSTFVVLFGNFRLGPDLSGGIILV
                     YEVDKRATAEMSRKGKSDWEMGQLLTVIRRRLNPDGLKEIVVRPFGQQQVEIVVPETD
                     PLEVQKIKELINTAGVLQFMIVAGETNDDNDLVELAKTHAERPDQERLKREVLNENGE
                     RVGFWATLAREDPKPGETDPPFRGLELIQTAILRGTGNGQILDLTQQERNTYAFNEPL
                     FKAYLAQHGLRDVDVLLKFDADFDVRGDDLSSSAASIDHETFSPCIHFTMTTGGSNKM
                     GFLTQNNLQRKLAIVFDSKLLSAPVIRSKIDTNGQITGRFTQEEVDFIVGILKSGSMP
                     VVMQKQPTSENQIGAILGRDTIEQGSWSVVVALGLILLFMVLYYRVSGAIASFALALN
                     LLLTVAIMVLLQAPFTLPGLAGLVLTVAMSVDANVLISERMREELAKGATLRMAIRNG
                     FDKALSAIIDGNLTTFFTALVLYAIGTDQVRGFGITLMLGNVTSMFTAIFCARVLLDI
                     GERTRWLKTLRMASFLTNPQIDWVKFFAPAAVASVILIVVGIVATVARGRGLFDIDLA
                     GGTSATFILKEPTPETEIRQRLEAAFKGMIDEATNTRVDHNVYQFKVNTERDQTVYKV
                     DSSLESVDKLKETIRNALRAKDGSDLLKTYNMELSTVTEVSTEKPPPSISAPPAIGAP
                     PAGGPALTPPAGAPPAEAPQTGTPAEAPPAKAPAATPDEKSKKTSEPAPGKSPETTEP
                     KKTEEDAKAKDSSECDPAQEAKSAEPAKTDQEKAEPAKTETKADQEKTDAAKTEPPKA
                     AEAPPAAAGATKAADAAKAGEPPPAATSPAGTPLATEPPLPGTPVEAPLKPLPKTKVT
                     AHLKFPGTPLSGFALHDRIVASGRAAIGREVNVLVKNPQWDSHDNSTFSEWDVEITGV
                     TQEQGEKILQNMKETLRKEVIWQTSNKIGGQVSADTRWRALGALAVSLLIIVAYVWFR
                     FQRVAWGLAAVAALAHDALVMLTCIALSYWCASALGFLGVEEFKISLSVVAAFLAILG
                     YSVNDTIVIFDRLREIRGKSPDVTRQMLNDAVNQTLSRNIILAGITVTVVVILYVFGG
                     PGIHAFAFALMTGVISGCYTTLVIAAPLLLWLLNRGAVQSTGKKVEKREGVAA"
     gene            complement(3992..4411)
                     /gene="yajC"
                     /locus_tag="VFB80_12185"
     CDS             complement(3992..4411)
                     /gene="yajC"
                     /locus_tag="VFB80_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZN34575.1"
                     /translation="MLAYFWLLLAQGGDAGGGAAPAGGAAAPGGAAGGGGLFETLFGG
                     SMILPLVATMALMYFLLMRPEQKRKKEMAELLANLKKNDKVVTIGGILGTVVSVSPEA
                     KFVTIRVDEGTKIRILRSAISQVGGPDEAEPDVKPAA"
     gene            complement(4551..5285)
                     /locus_tag="VFB80_12190"
     CDS             complement(4551..5285)
                     /locus_tag="VFB80_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN34576.1"
                     /translation="MSEQVTRGNLPHWYRPGFAHFVTYRLAGSIPNEVLRELRDEYSA
                     RLKSRPPAGMDRAEHKAKVHRFMFGKYDRVLDGQAAISWLADARLAQIVRENLYHHHG
                     TKYELLAYCIMPNHVHAVLQPLEGVVAAVGDAASVAEQLETPDRYSVLSKIMHSLKSY
                     TANRANELLGRSGVFWQKESYDHWIRDADELERIVAYVASNPVKAGLCDQSRRWQFSS
                     AYDRYQRDGSESALVGWLRDDWRTTP"
BASE COUNT          922 a         1707 c         1737 g          994 t
ORIGIN      
        1 tttttcggcc atcttgacga cagcgcggcc gatctagagc cgctggcggc gctcggcctg
       61 ggcgatgcgg cggctgaccc agatgatgtt gatcaggtcg ccggccaggc tgaacgagcc
      121 gagggccaat atcgcgaggc aggccgaaat ccaaaggccc agcagccagt ggagcgccag
      181 cagcacagcc gtcagcgcga gtacaaagac cgcgtgaccg ggcagcgccc gcagcgactg
      241 ccgccggctg cgccgccaat cgtccagctc gctggcggtt gccatggcgg gatctcacac
      301 ggaatgttgg cggcgacgct ggcagcgtcg tcaacgacta ggcggccact ccttcccgct
      361 tctcgacttt tttcccagtc gattgcacgg cgccccggtt gagcagccag agcaagagcg
      421 gcgcggcgat gaccagcgtc gtgtagcagc cggaaatcac gccggtcatc agggcgaagg
      481 cgaaggcgtg aatgcccggt ccgccgaaga cgtacaggat caccacgacc gtgaccgtga
      541 tgccggccag gatgatgttg cggctgagcg tctggttgac ggcgtcgttg agcatctgcc
      601 gcgtgacgtc ggggcttttg ccgcgaatct cgcggaggcg gtcgaagatg acgatcgtgt
      661 cgttcaccga atagccgaga atggccagga acgcggccac gaccgagagg ctgatcttga
      721 actcctcgac gccgaggaag cccagcgcac tggcgcacca gtagctgagg gcgatgcagg
      781 tgagcatcac cagggcgtcg tgggccaggg ccgcgaccgc cgccagtccc caggcgacgc
      841 gctgaaagcg gaaccagacg taggccacga tgatcagcag gctgacggcc aaggcgccca
      901 aggctcgcca gcgcgtatcg gccgagacct ggccgccgat cttgttcgac gtctgccaga
      961 tcacttcctt gcgcagcgtc tccttcatgt tctgaagaat cttctcgccc tgctcctgcg
     1021 tgacgccggt aatttcgacg tcccattcgg aaaaggtcga gttgtcgtgc gagtcccact
     1081 gcgggttttt caccaggacg ttcacctcgc ggccgatcgc cgcccggccg ctggccacga
     1141 tgcgatcgtg gagggcaaag ccggaaagcg gcgttcccgg gaacttgagg tgagcggtga
     1201 ccttcgtctt gggaaggggc ttgagcgggg cctcgacggg agttcccggg aggggcggct
     1261 cggtcgccaa cggcgtgcct gctggagatg tcgccgcagg cggcggctcg ccggctttcg
     1321 cagcatccgc ggctttcgtc gcgcccgctg cggcgggcgg cgcttcggcg gccttgggcg
     1381 gctccgtctt cgccgcatcc gtcttctcct ggtcggcttt agtctcggtt ttggcgggct
     1441 cggctttttc ctggtcggtc ttggcgggct cggcggactt cgcttcctgc gcggggtcgc
     1501 actcggagga atctttcgct ttggcgtcct cttcggtttt cttcggttcg gtggtctccg
     1561 gggactttcc gggcgcaggc tcactggtct tcttcgactt ctcgtcaggc gtcgctgcgg
     1621 gggccttggc cggcggcgct tcggccggcg tgccggtctg cggtgcttcc gctggcggcg
     1681 cgcccgcagg aggcgtcaag gccggaccgc ccgccggggg cgcaccgatc gcaggcggcg
     1741 cggaaatcga cggcggcggt ttctccgtgg acacctcggt gactgtgctc aattccatgt
     1801 tgtaggtctt gagcaggtcg ctgccgtcct tggctctcag ggcgttgcgg atcgtctcct
     1861 tgagcttgtc caccgactcc agcgagctat cgaccttata gaccgtctgg tcccgctcgg
     1921 tgttgacctt gaactgatag acgttgtgat cgacccgcgt gttggtcgct tcatcgatca
     1981 tgcccttgaa ggccgcttcc agcctctggc ggatttcggt ttcgggggtc ggctccttga
     2041 gaataaacgt cgccgaggtc ccgccggcca ggtcgatatc aaacaggccg cggccgcggg
     2101 ccaccgtggc gacgatgccg acgacaatca ggatgacgga ggccacagcc gcgggggcga
     2161 agaacttgac ccaatcgatc tgcggattgg tgaggaagct ggccatgcgg agcgtcttca
     2221 gccagcgggt ccgctcgccg atgtcgagca gcacgcgggc gcagaagatg gccgtgaaca
     2281 tgctcgtgac gttgcccagc atcagcgtga tgccgaagcc gcggacctgg tcagtgccga
     2341 tggcatacag cacgagggcc gtgaaaaacg tcgtcaggtt gccgtcgatg atcgccgaga
     2401 gggccttgtc gaagccgttg cggatggcca tccgcagggt cgcccctttg gccagctctt
     2461 ctcgcatccg ctcggagatg agcacgttgg catcgaccga catggcgacc gtcagcacca
     2521 ggcccgccag gccgggcagc gtgaacggcg cttgcagcag caccatgatc gccacggtga
     2581 gcagcaggtt gagggccagg gcgaacgacg cgatcgcgcc cgacacgcgg tagtacagga
     2641 ccatgaacag caggatcagg cccagagcca ccacaaccga ccagctcccc tgctcgatcg
     2701 tgtcgcggcc caggatcgcg ccgatctggt tctcgctcgt cggctgcttt tgcatcacga
     2761 cgggcatgct gccgctcttg aggatgccga cgatgaaatc gacctcttcc tgcgtaaagc
     2821 ggccagtaat ctggccgttg gtgtcgatct tgctgcgaat caccggggcc gagagcagct
     2881 tgctgtcgaa cacgatcgcc agctttcgct gcaggttgtt ctgggtcagg aaccccatct
     2941 tgttgctgcc gcccgtcgtc atcgtgaaat ggatgcacgg gctgaacgtc tcatggtcga
     3001 tcgaggccgc ggagctgctc aggtcgtcgc cgcgaacgtc gaagtcggcg tcgaatttga
     3061 gcagtacgtc cacgtcgcgc aggccgtgct gggccaggta cgccttgaac agcggctcgt
     3121 taaaggcata ggtgttgcgc tcctgctgcg tgaggtccag gatttgcccg ttgcccgtgc
     3181 cgcggagaat ggcggtctgg atgagctcca gacctcggaa cggcggatcg gtttcgcccg
     3241 gcttgggatc ctcgcgggcc agggtggccc aaaagcccac ccgctcgccg ttctcgttca
     3301 gcacttcgcg cttcagccgc tcctggtcag gacgctcggc gtgggtcttg gccagctcga
     3361 ccagatcgtt gtcgtcattg gtctcgccgg cgacgatcat gaactgcagg acgcccgcgg
     3421 tgttgatcag ctccttgatc ttctgaacct ccaggggatc ggtctccggc acgacgatct
     3481 ccacctgctg ctggccgaac ggccgcacca cgatttcctt caggccgtcg gggttcagcc
     3541 gcctgcggat gacggtgagg agctggccca tctcccagtc gctcttgcct ttgcgactca
     3601 tctcggccgt ggcccgctta tcgacctcat agacgaggat gatgccgccg ctcaggtcgg
     3661 gacccaggcg aaagttgccg aacagcacga caaaggtcga gaccagcgtc gtggcgagga
     3721 tcaggccgac tttccagccg taatccttca accgcgtggc ccgctcgaac agccagccga
     3781 gcacgaacga gaccacgatc aggcccagca ggaccgccgg aaagctggcg acgttgagcg
     3841 aatcgggcag gatcggcggc cagtcgaagt cgatcagccg gtagcgattt tcggccagga
     3901 tcgggctcag ggattggagc caatgaaaaa cgttcatcgc aagcatcccg ccaaagaaga
     3961 aggaagaatt tggccgcgag gcgaagcccc gctaggcggc cggtttgacg tcgggctccg
     4021 cctcgtcggg gccgccgacc tgggaaatgg cgctgcggag gatgcggatc ttggttccct
     4081 catccacccg gatcgttacg aacttggcct cgggcgagac ggagacgacc gtccccaaaa
     4141 tgccgccgat ggtcacgacc ttgtcgtttt tcttgaggtt ggcaagcaat tcggccatct
     4201 ccttcttccg cttctgctcc gggcgcatca gcagaaagta catcagggcc atcgtcgcga
     4261 ccaagggcag gatcatgctg ccgccgaaga gggtctcgaa caggccgccg ccacccgcgg
     4321 caccgccagg ggcggcggct ccgcccgccg gcgcggcacc gccacccgca tcgcccccct
     4381 gggccaatag caaccagaag tatgccagca acgcacaccc tccccgcggc aaaacaccgt
     4441 aacctccagc atcctaaggc gttgcggcaa atggaacaag gccgtaaccg ccgtagccgc
     4501 tgctgccagc agcggtttac gagtggcacg ccgacgctgg cggcgtcgag ctacggtgtc
     4561 gtccgccaat cgtcgcgcag ccagccgacg agtgcagatt ccgacccgtc ccgttgatat
     4621 cgatcgtaag ccgacgagaa ctgccaccgc cgcgactggt cgcacagtcc tgccttcact
     4681 ggattgctcg cgacgtatgc gacgatccgc tccagctcgt cggcatcgcg gatccaatgg
     4741 tcataagact ccttctgcca gaagacaccc gaccgtccca gcagctcatt cgcgcgattg
     4801 gctgtgtagc tcttgaggct gtgcatgatc ttgctaagca cgctgtatcg atcaggcgtt
     4861 tccaattgct cggcgacgct ggcagcgtcg cctacggcag cgacgactcc ttccagtggc
     4921 tggagcactg catgtacgtg attgggcatg atgcagtacg cgagcaactc gtatttcgtg
     4981 ccgtgatggt gatagagatt ttcgcgaact atttgtgcaa ggcgcgcgtc agctaaccag
     5041 ctgatcgcgg cctgtccgtc cagcacgcga tcgtattttc cgaacatgaa tcggtggacc
     5101 ttcgctttgt gttcagcgcg atccatgcca gccggcggcc tcgacttgag ccgcgccgaa
     5161 tactcgtcgc gcagctcccg gaggacctca ttcggaatac tgccagcgag tcggtaggtc
     5221 acaaaatgag cgaaaccggg ccgataccaa tgcggcaggt tcccgcgcgt tacctgctcg
     5281 ctcatggcga ccagcccgta gccgctgcgg ccagcagcgg cggattagtg acacgccgac
     5341 gctggcagcg tcggctacga
//
LOCUS       DASRJB010000462        11275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2094605, whole genome shotgun sequence.
ACCESSION   DASRJB010000462 DASRJB010000000
VERSION     DASRJB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094605"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFB80_12195"
     CDS             <1..308
                     /locus_tag="VFB80_12195"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HZN34577.1"
                     /translation="ELQAAIEGLSALKRPCRIELFTDSEYVRKGLAEWMPGWKRNGWR
                     RREKGKLKPVANAELWQQLDELLAKHEVKFTRVAGHSGHPENDRCDELAVQAYQKFL"
     gene            complement(326..1594)
                     /locus_tag="VFB80_12200"
     CDS             complement(326..1594)
                     /locus_tag="VFB80_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34578.1"
                     /translation="MLLTRLFGCLILSALLLNAAVAADWLQFRGTAGQGISTETGLPT
                     TWSASENIVWKTAVPGAGTSSPIFVGDKIFLTAYRGYNVPGEGGGDMSQLERLVLCLN
                     RKDGKLVWSTPVPSKLPEQETIRDDHGYASSTPVSDGQRLYVFFGKAGVFALDLAGKI
                     LWQADVGDGLNYWGSAASPILHGNLVIVNASVESQSLVAINKNTGKEVWRARGIKESW
                     NTPIVVKTAAGKPEVVVAIMGDVLGFDPASGEELWSCDTDIGWYMVPSLVAEKDVVYC
                     IGGRGSGGGLAVRVGGRGDVTDTHRLWIAQKGSNVSSPLVYGGHLYWAHESLGIVYCA
                     NLATGRVVYEERLPRAGQFYSSPVLADGKIYYTTRNGRTFVVAAQPKFELLATNDLGE
                     RSTFNACPVAADGRLFIRSDKTLYCLDQLR"
     gene            1703..2083
                     /locus_tag="VFB80_12205"
     CDS             1703..2083
                     /locus_tag="VFB80_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN34579.1"
                     /translation="MLIAQLNHVALHVADVERSVQFYRDVLQLTPIPRPAFTFPGAWF
                     RLGVDQELHLIGERTKEVVSHNRGNHFALLVDDFNAWEQRLTELGVTFLPRRTRPDGA
                     LQLFIIDPDGHYLELCTPPGAVAP"
     gene            complement(2100..2894)
                     /locus_tag="VFB80_12210"
     CDS             complement(2100..2894)
                     /locus_tag="VFB80_12210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN34580.1"
                     /translation="MIDLKGHSALVTGSTKGVGRSIAEAFAAAGCHVVLHGRQRDANA
                     EEALSACRSKGVHADYVISDLMGETIPAVDALFASAIHALPQIDILVNNAGTYIDKPF
                     LEMDWQTYDRTMRLNVAAPYFLTQRFAQHWIARQVAGRVLMIGSINGRLAEPTHSCYD
                     SSKGAIDALVRSLCVSLAPYNIRVNGLAPGLVRTPLTQVIDRDERFRRWMELHTPNGQ
                     VPHSDVCGSGAVYLCSDDAWHVHGQMLLVDGGMSAWQQPDMPDSWQ"
     gene            complement(2932..3288)
                     /locus_tag="VFB80_12215"
     CDS             complement(2932..3288)
                     /locus_tag="VFB80_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34581.1"
                     /translation="MKPHNVAWLLAYLAVLALVVGGLIYGRAQALAIYGSEHAQADWD
                     QWRARAKKTATQPGPVKRREPKSAEPPALVLMRDYFLVCSLLAVLLSSVLFATFMFFI
                     RGAMSSPGSRLPTPDS"
     gene            complement(3293..4594)
                     /locus_tag="VFB80_12220"
     CDS             complement(3293..4594)
                     /locus_tag="VFB80_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN34582.1"
                     /translation="MSLSRGRLVLASTLLAVSCLAARAEDPRAWATKHEGELVELYQH
                     FHAHPELSFEEVETAARVAKELRALDIEVTEKVGRLGVVGILKNGAGPTVMIRTDLDA
                     LPVTEQTGLAYASKVKVKSDAGESGVMHACGHDIHITNLIGTARWLAAHKDQWRGTVM
                     FLGQPAEERVGGAKVMLEDGLFTRFPKPDYAIALHCASNLATGKVGVTPGYALANTDS
                     VDITMVGRGGHGAYPHTTIDPIVMAAQLVLDLQTIVSREIKPLEPCVITVGSIHGGTK
                     HNIIGNECKLQLTVRSYTDEVREQMKAAIVRKAKAVAAGAGAPEPKVAFIDGTPAMFN
                     DEKLAARLHAVFVKELGAANVETPEKELGGEDFSMYGRAGVPILMFRLGTVEPQRLAR
                     LTQLGQEPPSLHSPLYYPDAEQSLITGVQTTVAATLELLKK"
     gene            complement(4767..5942)
                     /locus_tag="VFB80_12225"
     CDS             complement(4767..5942)
                     /locus_tag="VFB80_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN34583.1"
                     /translation="MIRTFSLVACLLSACAAVAWSQQPRPTQPVEAKAASELSAQLVA
                     DDLARYVDYALIDMSKSLIADNGLGIDVAPPEAALRAQLGLDEGAGLVVTSAPEESLG
                     AKAGLRVHDVILKFGDQQSGEIEKLKEWLGAADGKVVTLKVLRGGKPLEIQVMPKKPE
                     SARVRVRKLTDLAEGWKHLAVASQERYRIGVSLSEADDTLRAQLRLAAGEGLVVTEVI
                     KDSAAAAAGMQVNDVLTVLDGKRLTTVDAINAQVQEIKDRLVDLRLLRAGEERILQVA
                     PRKSVEAAFHDRAVRYWDMQSCQKCHAQPFDDAQAAHLMMKWKLGAHHSAWTDGEQLH
                     LYGKLFNLNEKPAAEGDSQKQLEVLKSQLAEMQKTLAALESSLAASKEEQPQPQEKK"
     gene            complement(5982..6623)
                     /locus_tag="VFB80_12230"
     CDS             complement(5982..6623)
                     /locus_tag="VFB80_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34584.1"
                     /translation="MNTKFTLDDAMRAQLDRLVAGELPENDRRSLLAWLDEDASRWRN
                     CALAFLEAQTWEAASAGWPAAMKAAPAPINSIQNQSSLSWQRLLTLAAAAAVLFFAGH
                     LSARVWRPPAAPQREVAQPTGPQGQKGPVLASVPVRTNPSLPATLQIAVSPVADDAPA
                     APALSDYERKQWEKSGFDVQEELRYLPARLPDGTPVLVPVNKLHVKLKSNRFS"
     gene            complement(6620..7189)
                     /locus_tag="VFB80_12235"
     CDS             complement(6620..7189)
                     /locus_tag="VFB80_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN34585.1"
                     /translation="MTAADQSPPAARSPGLDWERCLPLHEGWLRRVIAARTGEPQSVE
                     EVWQQVALAAIEQRWPLTDPAKVGPWLHRLAVIAAARYCRQLGRQRRAVQGLAAARHS
                     GGGAAAAPDPLRLLMRRERVQLTRQALVAIAPRDAEILLLKYGERWSYRQIAEHLGIT
                     DRAVDSRLLRARDSLRRALAALGIDEDEP"
     gene            complement(7238..8623)
                     /locus_tag="VFB80_12240"
     CDS             complement(7238..8623)
                     /locus_tag="VFB80_12240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34586.1"
                     /translation="MASRSVFKNSALLSGLVTQEQLDQAVATAGTGPGGSPAPMVEVD
                     DAALALRLIEMGILTAYQADQIKAGRTKFHLGQGAYVVTDFIGQGGMGQVFKGVHCVM
                     GRDCAIKVLPLHKATPEAIANFMREVRTQAQLDHPNLVRAYDAGRDGNTHYLVVEYVP
                     GVDLRRLVRGQGPLTQQQAASVILQAARGLDYAHKRGLVHRDVKPGNILVTAEGISKV
                     SDLGLAGFIHDAGNDPRAGKIVGTADYLAPEQIRNAGDVTHLSDIYSLGCTLYYAVCG
                     KVPFPGGKANEKARRHLNDTPWHPRRFSAELNEEFVDVIADMMEKEPKVRIQTAAEVV
                     ARLELFAGEASPISARTNIRSPWTAPPLPGGSDDDKDVLQDTDGGDFSASGAEASTSQ
                     NSQGTSAVASAGHETPSSKSRPRPLPMAQPLQLPVLTPTAIVALTLMISIPVSMLVGA
                     LIGAVVASWWR"
     gene            9108..9536
                     /locus_tag="VFB80_12245"
     CDS             9108..9536
                     /locus_tag="VFB80_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HZN34587.1"
                     /translation="MEMAVATPVSHAPLAHEDRRQTAHRVAADMFSHQPDWVTFFREV
                     LGVDGLIRKLFTNPDDLAAFEKTAEYAEIQNMLARLRERNGAAADGKEPTRVITVRLP
                     KSLHESLRAEAHDRNTSMNQLCISKLLMVIDAEIAAAASE"
     gene            complement(9617..10058)
                     /gene="rnpB"
                     /locus_tag="VFB80_12250"
     ncRNA           complement(9617..10058)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB80_12250"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10243..>11275
                     /locus_tag="VFB80_12255"
     CDS             10243..>11275
                     /locus_tag="VFB80_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN34588.1"
                     /translation="MKRTLLFCLLSATVGALAATAWHQTGVFRRATAQEPLLRQPRGN
                     TLELPPPPVPPSSRRPAGGLSPAISSAAPSGLDEFAPEERTNILVYERANRSVVHITT
                     RAAQRDLLFTEVAEGAGSGSILDRQGHILTNYHVVEGAQEIRVTLHTGESFEGTVVGS
                     DPPNDMAVLRVNAPQDQLVPMQLGDSSRLKVGQIVYAIGNPFGFERTMTTGIISGLNR
                     PLQTRAGRTMKSIIQIDAALNRGNSGGPLLDTRGRLIGMNTAIASSTGENTGVGFAIP
                     ADTIKRVVPQLITTGKVIRPEAGISRVQQTESGLVIVTLVPGGAAERAGLRGFKVVRE
                     QKRRGPFTYE"
BASE COUNT         1908 a         3763 c         3618 g         1986 t
ORIGIN      
        1 tggagctgca agccgcgatc gaagggctgt ccgccctcaa gcggccctgc cgcatcgagc
       61 tgttcaccga cagcgaatac gtccgcaagg gcctcgcgga gtggatgccc ggctggaagc
      121 gcaacggctg gcggcggcgc gagaagggca agctaaagcc cgtcgccaac gccgagctct
      181 ggcagcagct tgacgagctg ctcgccaaac atgaggtcaa gttcactcgc gttgccggcc
      241 acagcggcca tccggaaaac gaccgatgcg acgaactcgc cgtgcaggcg tatcaaaagt
      301 tcttgtgagc tggcggctgc gtttgctacc gcaactgatc caggcagtac agcgtcttgt
      361 cagatcggat gaacagccgg ccgtcggcgg cgacggggca ggcgttgaag gtgctccgct
      421 cgccgaggtc gttcgtcgcc agcagctcga acttcggctg ggcggcgacc acgaacgtgc
      481 ggccattgcg ggtcgtgtag taaatcttgc cgtcggcgag cacgggcgac gaatagaact
      541 gaccggcccg cggcagccgc tcttcataca ccaccctgcc cgtggccagg ttggcgcagt
      601 agacgattcc cagcgactcg tgggcccagt acagatggcc gccgtacacg agcggcgacg
      661 agacgttcga gcctttttga gcaatccaga ggcgatgcgt atcggtcaca tcgccccgtc
      721 cgccgacgcg caccgccagc ccgcccccgc tgccgcggcc gccgatgcaa tacacgacgt
      781 ctttctcggc caccaggctg ggcaccatgt accagccgat gtcggtgtcg cacgaccaaa
      841 gctcctcgcc ggacgccgga tcgaatccca gcacatcgcc catgatcgcc acgacaacct
      901 ccggcttgcc ggcggcagtc ttgacgacga tcggcgtgtt ccacgactcc ttgatcccac
      961 gggcccgcca gacttccttg ccggtgttct tattgatggc gacgagcgac tggctctcga
     1021 cgctggcgtt cacaatgacc aggttgccgt gcaggatcgg cgaggcggcg ctgccccagt
     1081 agttgagccc gtcccccacg tcggcctgcc agagaatctt gcccgccagg tccagcgcga
     1141 aaacgccggc cttgccgaag aagacataga gtcgctggcc atcgctgacg ggtgtgctgg
     1201 aagcgtagcc gtggtcgtca cgaatcgtct cttgttctgg cagcttcgac ggcacgggcg
     1261 ttgaccagac gagcttgccg tcttttcgat tgaggcagag cacgagccgc tccagttggc
     1321 tcatgtcgcc gccgccttcg ccgggcacgt tatagccgcg ataagcagtg aggaagatct
     1381 tgtcgccgac gaagatcggg ctcgacgtgc cagcgcccgg aacagcggtc ttccagacga
     1441 tgttctcact agccgaccag gtcgtcggca ggcccgtttc ggtgctgatg ccctgacccg
     1501 ccgttccacg gaattgcagc cagtccgcgg caacggcggc attcaacagc agggcagaca
     1561 ggatcagaca tccgaaaagc cgtgtcagca gcatgaaaat ctcccggaat gatattgccc
     1621 aacggtaaat ggccgccggc cggccaggtg tgacataata atcgccaccc ctcaccgccg
     1681 accactcacg actcactgcc acgtgctcat cgcccaactc aaccacgtcg ctttgcacgt
     1741 cgccgacgta gaacgcagcg tgcagttcta ccgcgacgtg ctgcagctga cgccgatccc
     1801 gcggccggcg ttcacttttc ccggcgcgtg gtttcggctg ggcgtcgatc aggagctgca
     1861 tctgatcggc gagcggacga aggaggtcgt ttcgcacaac cgcggcaatc actttgcgct
     1921 cttggtggat gacttcaacg cctgggagca gcggctgacc gagctggggg taacgttcct
     1981 gccccgtcgc acccggcccg acggcgcgct ccaactcttc atcatcgacc ctgacggaca
     2041 ctacttagaa ctctgcacgc cgcccggggc cgtcgcgcct tgaccgacat ttgcggttct
     2101 cattgccagc tatccggcat gtccggctgc tgccaggcgc tcatgccgcc atcgaccagc
     2161 aacatctgac cgtgaacgtg ccaggcgtcg tcgctgcaca gatagaccgc gccggagccg
     2221 cagacgtcgc tgtgcggaac ctggccattg ggcgtatgca attccatcca gcggcgaaaa
     2281 cgctcgtcgc ggtcgatgac ctgtgtcagc ggcgtgcgga ccaggcccgg cgccaggccg
     2341 ttgacgcgga tgttgtaggg cgcgagcgaa acgcacagcg agcgaacaag cgcgtcgatc
     2401 gcgcccttcg acgagtcgta gcacgagtgc gtcggctccg cgagccgccc gttaatcgag
     2461 ccgatcatca gcacgcggcc ggcgacctgc cgtgcgatcc agtgctgggc aaaccgctgc
     2521 gtcagaaagt acggcgcggc gacgttcagc cgcattgtgc ggtcgtaggt ctgccagtcc
     2581 atctccagga agggcttgtc gatgtaggtg ccggcgttgt tgacgaggat gtcgatctgc
     2641 gggagcgcgt ggatcgccga cgcgaacagc gcatcgacgg cgggaatcgt ttcgcccatc
     2701 aagtcgctga tgacatagtc ggcgtgcaca cccttgctgc ggcaggcgct tagcgcttcc
     2761 tcggcgttgg cgtcgcgctg gcggccgtgc agcaccacat ggcaaccggc agcagcaaag
     2821 gcctcggcaa tcgagcggcc cacgcccttg gtcgagccgg tgacgagagc cgagtggcct
     2881 ttgaggtcga tcatgggccg ggggcggggg tcgggagtct ggggtcgcga gtcaggagtc
     2941 gggagttggg agtcgcgagc cgggagagct catcgctccg cgaatgaaga acatgaacgt
     3001 agcaaacagc acgctggaga gcagcacggc cagcaaggag cagacgagaa aatagtcccg
     3061 catcaggacc agtgccggcg gctcggcgct tttgggctcg cgacgcttca cgggcccggg
     3121 ctgagtcgcc gttttctttg cgcgagcgcg ccactggtcc caatcagcct gggcatgctc
     3181 ggagccatag atcgccagcg cttgcgcgcg gccgtagatc agtccgccga ccaccagcgc
     3241 gagcactgcg aggtaagcca gcagccaggc gacgttgtgc ggtttcattc cgctacttct
     3301 tgagcagctc gagtgtcgcg gcgaccgtgg tctgcacgcc ggtaatcagg gactgctcgg
     3361 cgtcgggata atagagcggc gagtggagcg acggcggctc ctggccgagt tgcgtcaggc
     3421 gggccagccg ctgcggctcc acggtgccga ggcggaacat caggatcggc acgccggcgc
     3481 ggccgtacat actgaagtct tcgccgccca gctccttctc gggcgtctcg acgttggccg
     3541 cgccgagctc cttcacaaac acggcgtgca gacgggccgc tagcttttcg tcgttgaaca
     3601 tggccggcgt gccgtcgatg aacgcgactt tcggttcggg cgctccggcc ccggcggcca
     3661 cggccttggc cttgcggacg atggcggcct tcatttgttc gcggacctcg tcggtgtagc
     3721 tgcggaccgt aagctgcagc ttgcactcat tgccgatgat gttgtgcttg gtgccgccgt
     3781 ggatcgagcc aaccgtgatc acgcagggct ccagcggctt gatctcccgg ctgacgatcg
     3841 tctgcaagtc gaggacgagc tgggccgcca tcacgatggg gtcgatcgtc gtgtgcgggt
     3901 aggctccgtg gccgccacgg ccgaccatgg tgatatcgac gctgtccgtg ttcgccaggg
     3961 catagccggg cgtcacgccg accttgccgg tcgccaggtt cgaggcgcag tgcagggcga
     4021 tcgcatagtc cggcttggga aaccgcgtga acagcccgtc ttccagcatc accttggccc
     4081 cgccgacgcg ctcctccgcc ggctgcccga ggaacatgac ggtgccgcgc cactggtctt
     4141 tgtgggccgc cagccagcgg gccgtgccga tcaggttcgt gatgtgaatg tcgtggccgc
     4201 aggcgtgcat cacgccgctt tcgccggcgt cgctcttgac cttcaccttc gacgcgtagg
     4261 ccagaccggt ctgctccgtc accgggaggg cgtcgaggtc ggtacggatc atgaccgtcg
     4321 gcccggcccc atttttcagg atgccgacga cgcccagccg gccaactttc tcggtcactt
     4381 caatgtcgag agcccgcagc tctttcgcga cgcgggccgc cgtttccact tcctcgaacg
     4441 acaattccgg gtgggcgtga aaatgctggt acagctcgac cagctcgccc tcgtgctttg
     4501 tcgcccaggc tcgcggatct tcggcccgcg cggcaaggca ggagacggcc agcaacgtcg
     4561 aagcgagaac caatcggccg cgcgacaagc tcatgagtgt ctcccagtcg atggaaattc
     4621 ttggagctgc ccttggcaaa ccagggcgac ttgggcttta atcaacccaa gtcatcgctc
     4681 ttattctcat ccggcaggtt ttcgcaatta tctcgcggaa acctgcctta ttttatgcgc
     4741 gctgggccgc gcttccgcca gggtggctat ttcttctcct ggggctgtgg ctgctcttcc
     4801 ttgctggcgg cgagcgacga ctccagcgcc gccagcgtct tttgcatctc ggcgagctgc
     4861 gatttgagca cctcgagctg cttctgcgaa tctccctccg ccgcgggctt ctcattcaga
     4921 ttaaatagtt ttccataaag gtgcagctgc tcgccgtcgg tccacgccga atggtgcgcg
     4981 ccgagcttcc atttcatcat caggtgggcg gcctgggcat cgtcaaaggg ctgggcgtgg
     5041 cacttctggc agctttgcat gtcccaatac cgcaccgcgc ggtcgtgaaa cgccgcctcg
     5101 accgacttcc gcggcgcaac ttgcaggatc cgctcctcac cggcccgcag cagccgcaaa
     5161 tcgaccaagc ggtccttgat ctcctggacc tgggcattga tagcatcgac ggtcgtcagc
     5221 cgcttgccgt cgagcaccgt cagcacgtcg ttgacttgca tgccggccgc ggccgcggca
     5281 ctgtccttga tgacctccgt gacgacgagc ccttccccag cggcgagccg cagctgggcc
     5341 cgcaacgtgt cgtcggcctc cgacagcgaa acaccgatgc ggtatcgctc ctggctggca
     5401 accgccaggt gcttccagcc ctcggccagg tcggtgagct ttcgcacgcg aaccctggcc
     5461 gactccggct tcttgggcat gacctgaatc tccaggggct tgcctccgcg aaggactttc
     5521 agcgtgacca ccttgccgtc ggcggcgccc agccactcct tgagcttttc gatctcgccg
     5581 gactgctgat cgccaaactt caaaatcacg tcgtggactc tcaggcccgc cttcgcgccg
     5641 agcgactctt ccggagcgct cgtcaccacc aggcccgcgc cctcgtccag ccccagctgc
     5701 gcccgcaggg ccgcctcggg cggggcgacg tcgattccca gcccattatc ggcaatcaag
     5761 gacttcgaca tgtcgatgag cgcataatcc acgtagcggg ccaagtcgtc cgcgaccagt
     5821 tgggcagaca attccgaagc ggccttggcc tcgaccggct gagttggccg cggctgttga
     5881 ctccaggcga cagccgcaca ggccgacaac aggcaggcga cgagagagaa cgttcgaatc
     5941 atggtcggac tccttgagga gattgctgaa tcggggaaag gctaggagaa gcgattggac
     6001 ttgagcttga cgtgcagctt gttgaccggc acgagcaccg gcgtgccgtc gggaagccgg
     6061 gccgggaggt agcggagctc ttcctgcaca tcgaagccgc tcttctccca ttgcttgcgt
     6121 tcgtagtcgg acagtgctgg agccgccggc gcatcgtccg caacgggtga cacggcgatc
     6181 tgcagcgtgg ccggcaggct gggattcgtg cgaaccggta cgctcgccag caccggtcct
     6241 ttttggccct gcggcccagt cggctgcgcg acttcacgct gcggcgcggc cgggggccgc
     6301 caaacgcgcg ccgacaagtg gcctgcgaaa aacagcacag cggcggctgc cgccagcgtc
     6361 agcagtcgtt gccaggacag gctcgactgg ttttggatgc tgttaatcgg cgcgggtgct
     6421 gccttcatcg ccgccggcca gcctgcgctt gctgcttccc aggtctgggc ctccagaaat
     6481 gccagcgcgc agttccgcca gcggctggcg tcttcgtcga gccaggcgag caggctccga
     6541 cggtcgtttt cgggcagttc gcccgccacc aggcgatcga gctgcgcccg catggcgtcg
     6601 tcgagcgtaa actttgtatt catggttcgt cctcatcaat tcccaaggca gccagcgctc
     6661 ggcgcaggga atcgcgcgcc cgcagcagcc ggctatcgac ggcccggtcg gtaatgccca
     6721 agtgctcggc gatctggcga tagctccagc gctcgccgta tttcagcagg aggatttcag
     6781 cgtcccgcgg cgcgatggcc acgagcgcct gccgcgtcaa ctggacccgt tcgcggcgca
     6841 tcaacagccg cagcggatcg ggcgcagcgg cggcgccgcc gccggaatgc cgcgcggcgg
     6901 ccagcccttg cacggcccgc cgctgccggc ccagttgccg gcagtagcga gcggctgcga
     6961 tgaccgccag gcggtgcagc cacggtccga ccttggccgg atcggtcagc ggccagcgct
     7021 gctcgatcgc cgccaaggcg acctgctgcc agacttcctc gaccgattgt ggctcgccgg
     7081 tgcgggcggc aatcacccgc cgcagccagc cctcgtgcag cggcaggcag cgctcccagt
     7141 cgagtcctgg tgagcgtgca gcaggcggcg attggtcggc agcggtcatc actgcctcaa
     7201 gagttcccgc agccgacaaa atcccgcagc gaattcccta ccgccaccac gaggccacga
     7261 ccgccccgat gagcgccccg acgagcatcg acacgggaat cgagatcatc aaggtcaggg
     7321 ccacgatggc ggtcggcgtc agcaccggga gctgcagcgg ctgggccatc ggcagggggc
     7381 gggggcgact cttggaggag ggggtctcgt gtccggcgga cgcgactgcc gaagtgccct
     7441 gcgaattctg gctcgtgctg gcctcggccc cgctggccga gaagtcgccg ccgtcggtgt
     7501 cttggagcac gtccttgtcg tcgtccgagc cgccgggcag cggcggcgcg gtccagggcg
     7561 agcggatgtt cgtcctggcg ctgatcggcg atgcctcgcc cgcaaacagt tccagccggg
     7621 ccacgacttc ggccgccgtt tgaatacgga ctttgggctc cttctccatc atgtcggcga
     7681 tgacatcgac gaattcctca ttgagctcgg cgctaaatcg ccgtggatgc cagggcgtgt
     7741 cgttcaggtg ccggcgggcc ttctcgttgg ccttgccgcc gggaaacggg accttgccgc
     7801 agaccgcgta gtagagcgtg cagccgagcg aatagatgtc gctaagatgc gtgacgtcgc
     7861 cggcgttgcg aatctgctcg ggggccagat agtcggccgt gccgacgatc ttccccgcgc
     7921 gcgggtcgtt gcccgcgtcg tgaatgaacc ccgccagccc caggtccgag accttggaga
     7981 taccctcggc ggtgaccagg atgttgcccg gcttcacgtc gcggtgcacc aggccccgtt
     8041 tgtgggcata atcgaggccg cgggccgcct gcaggatgac gctcgccgcc tgctgctgcg
     8101 tcagcgggcc ttgtcctcgg accagccgcc gcaaatcgac tcccggcaca tactccacga
     8161 ccaggtagtg cgtgtttccg tcgcgaccgg cgtcgtaggc tcgcacgagg ttcggatgat
     8221 cgagctgcgc ctgcgtccgg acttcgcgca tgaagttcgc gatcgcctcg ggcgtggcct
     8281 tgtggagcgg caggaccttg atcgcgcagt cgcggcccat gacgcagtga acgcccttga
     8341 acacctgccc catgccgccc tggccgatga agtcggtgac gacgtaggcc ccctggccga
     8401 gatgaaactt ggtccggccg gccttgattt ggtccgcctg gtaggccgtg aggatgccca
     8461 tctcgatcag ccgcagggcc agcgcagcat catcaacttc gaccatgggg gcgggcgagc
     8521 cgcctgggcc cgtcccggct gtagccacgg cctggtcgag ctgctcctgc gtcaccaggc
     8581 cgctgagcag ggccgagttt ttgaatacgg agcgggaggc catggtcttt ggtgctgtca
     8641 ctgaggcgcg acgccgatta gacggagctg cgcccaatcc gttccgccgc agcctacttc
     8701 cgctcgaccc cacgcgccaa ggatgtgaag aaaagggagg gtgaggctcc cgccgagccg
     8761 cgaaccgcgg gtgaagccgc ggctcagcag gacttcgccc tcccgatatg gcgacaggtt
     8821 tacccagtgt gaaacttcgc ccgatttctg ccgtccgcta gctggacact tggccgaatg
     8881 cgactttggc tcctgcctaa aaatcaggca cccggccctc tgctgcccct ctaggcaggc
     8941 ctgaccgaaa ttgagcacgt gggccggccg cgtcgtaagt gcaagcagtg caagcacctg
     9001 caagctgggc ttgactccat tcgcagattt tgggaaatcg ccgcccgtgg gggcagcgag
     9061 tccgattttg tcccgccaat tacgacccgt tttggaagga gccagacatg gaaatggccg
     9121 tagcaacccc cgtctcccac gccccgctcg cccacgaaga ccgccggcag accgcccatc
     9181 gtgtcgcggc tgacatgttc agccatcagc ccgactgggt cacgttcttt cgcgaagtgc
     9241 tcggcgtgga cggcctgatc cggaagctat tcacgaaccc ggacgacctc gccgcgttcg
     9301 agaagaccgc cgagtacgcc gagattcaga acatgttggc ccgcctccgc gagcggaacg
     9361 gcgcggccgc cgacggcaag gagcccacgc gggtcatcac cgtgcggctg cccaagagcc
     9421 tgcacgagtc gctgcgggcc gaggcccacg atcgcaacac cagcatgaac cagctctgca
     9481 tcagcaagct gctcatggtg atcgacgcag agattgcagc ggctgcgtcg gagtgagtca
     9541 gccggccgat tagcttgact ggcacagaat ggagaaacat cgggaggctg actcctcccg
     9601 ctcgccgaaa aagcggtctc ggagcgggcc tgtaagccgg gttctgtccc cgccgccctt
     9661 gcagacggcg gaggacggcc atttttctag gacgccggtt gcccggcgtc tcgagcagcc
     9721 tacccgagcg ccgaatcggg ccggaccggc ccgcgcggac acttgcgcat ccgcggacgc
     9781 tctgcttggc cttgctccag gtggggtttg ccgagccagc cgggtcgccc cggctgctgg
     9841 tgcgctctta ccgcaccgtt tcacccttac cgcggcaagc gaaaaagacg ggggcccatc
     9901 gctcgccggc ggcggtctgc tctctgttgc actttcccga gccttgcggc cggtcgccgt
     9961 tagcgaccac cttcgtcctg tggagcccgg actttcctct cccgggaagg aatgccaagg
    10021 cattccctac aaccggcagc ggccgtccgg cccgctccga gaccgcatgc acgatatcgg
    10081 aatggctacc aattagatag acgctttata atgccgggtg gttcggggaa agctccaaga
    10141 accgaagaac caaagaacca aatggcggca acttccgcag gttcggcacc ttcggttttc
    10201 ggttcttttg gttctttggt tcttcgattt tccaacaatg ccatgaaacg caccctcctg
    10261 ttttgcctgc tctccgccac cgttggcgca ttggccgcga cggcctggca tcaaacgggc
    10321 gtcttccgcc gggcgacggc ccaagagccg ctgctccggc agccgcgggg caatactctg
    10381 gagctgccgc cacccccagt tccgccttcg tcgcgtcgac ccgcgggcgg cctttcgcct
    10441 gcgatcagtt ccgcagcgcc gtcgggcctc gacgagttcg cgccggaaga gcgcacgaac
    10501 atcctggtct atgagcgggc caaccgcagc gtcgtccaca tcacaacccg ggcggcgcag
    10561 cgcgatttgc tattcaccga ggtcgccgaa ggcgcgggca gcggctcgat cctcgaccgg
    10621 caggggcaca tcctgacgaa ctaccacgtc gtggaagggg cccaggagat ccgcgtcacg
    10681 ctgcacacgg gcgagtcgtt cgaagggaca gttgtcggat ccgatccgcc gaacgacatg
    10741 gccgtgcttc gcgtcaatgc tccgcaggac cagctggtgc cgatgcagct gggcgattcg
    10801 tcgcggctga aggtcgggca gatcgtctat gccatcggca acccgtttgg atttgagcgg
    10861 acgatgacga ccggcatcat cagcggcctc aatcgcccgc tgcagactcg cgccggccgc
    10921 accatgaagt cgatcatcca gatcgacgcc gcgcttaacc gcggcaactc cggcgggccg
    10981 ctgctcgata cccgcggccg attgatcggc atgaataccg ccatcgccag ctcgaccggc
    11041 gaaaacaccg gcgtcggctt tgcgatcccg gccgacacga tcaagcgggt cgtgccgcag
    11101 ctcatcacga ccggaaaggt gattcgtccc gaagccggca tcagccgcgt gcaacagacc
    11161 gaaagcggcc tggtgatcgt gacgctggtt cccggcggcg cggccgaacg ggcgggcctg
    11221 cgcggcttca aggtcgttcg cgagcagaag cgccgcgggc ctttcaccta cgaag
//
LOCUS       DASRJB010000463         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3229243, whole genome shotgun sequence.
ACCESSION   DASRJB010000463 DASRJB010000000
VERSION     DASRJB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229243"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3282
                     /locus_tag="VFB80_12260"
     CDS             <1..>3282
                     /locus_tag="VFB80_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34589.1"
                     /translation="ADRATFDTTAANEKIETALSTDGSLSLQWRPKVAEGMVDQALTA
                     RSSVAFDVREDSLRVAWQVRLEFGRAFRDAFSLTAPADYLIESVTGDNVRGWTVKKEA
                     DKQRIDVTLLKPVQGGETLTLQLAKRGRVGQGELAEFEAPLIQVEGAALEQGEIAVRK
                     SPRLDLRTVAAVSLVRADAGGQTAAVEQLADAADAAVLLVKPYQTFRFVKPPFRLALA
                     ASSMPQGATADIKAALQLAERGTTLDAAITFRPQGQPLYQVRLYLPEGFSLDRLAPSD
                     LEWAITTDNNRQLLTVYLLDGRSDQFTLTLLGKIAAPPQPPAPPAPAPATGQPSRSLP
                     LPRLEILDVQKQEGEIVLVPDPDTDVRLDQVTNADTAPLTGGPGWMKGEQQPLAKAVL
                     RYRVPSHSATITLTPRTPIVSARSISNVKITPRSIEETILIDFQIEQAGIRRVSFLVP
                     QHLAKARLKTLLLKQKTIETATGAAGSPPAGMVRVRLELQDYVRGRYSVLLEHDRLLT
                     SGKQRIDLPVIETGRTDRRLVAIENAGRDEVVIGPNDAAGLEPINRQQQAWRDLAAVL
                     GESVTQAYSALDNAPQPNLSFTTVERQQAERPRARIGLGTTLLVVDASGAYRALVQYR
                     LTNAEEQFLEVTLPAGARLWTVQVAGEPVKPVQTVPPLAHVVRIPLVKTGEGEGDYLV
                     ELKYGGKLPAIHGLAPVDFPLIRETSVGVELSQVRLLLPESQQWFNFGGTLRRVLEEE
                     ELQKGFHAYLSERIREATEALSSANDFTKVRAAANLKQSRQMFDYNRQYQAANNLKQL
                     DADLRNETLLGRAEQQAQSELAQQQRGEEADNRSRLNSAWSKQGVTRSKNIVDDLGSN
                     FDGAAAGDAGKMKGAEVFNPAFLDQNKLRREEEKGEKDAAKDLPAGKPGESQPEGKGS
                     GRYFRGNNQPMDESGGGKQGQGQADQKAPDIADKKELDQLQQKLQQEVEGREQTRRSS
                     GDRAQQLQRYQQNLDFQNNAQQPLNNYALPMQPQAGGATPQLGNLDGRLGQPGGGQGG
                     GQFGGYGGVPNSGGNRSGSGQSSDGGLGNPNGPMNQPQPAQDPNAAPAAPQPPPGDAE
                     GYMQI"
BASE COUNT          621 a         1113 c         1108 g          440 t
ORIGIN      
        1 tcgccgaccg cgcgacgttc gacacgacgg ccgccaacga gaagatcgag acagcgctca
       61 gtactgacgg ttcgctcagc ttgcagtggc gtcccaaagt cgccgagggc atggtcgatc
      121 aggccctcac ggcccgctcc agcgtggcct tcgacgtccg cgaagattcg ctccgcgtgg
      181 cgtggcaggt gcggctggag ttcggccggg ccttccgcga cgccttctcg ctgaccgccc
      241 ccgcggacta cctcatcgaa tcggtcaccg gcgacaacgt ccgcggctgg accgtcaaaa
      301 aggaggccga caagcagcgg atcgacgtga cgctgctcaa gcccgtgcaa ggcggcgaga
      361 cgctgacctt gcagctggcc aaacggggcc gcgtgggcca gggcgaactg gccgagttcg
      421 aagccccgct gatccaggtc gaaggcgccg cactggagca gggcgagatc gccgtccgca
      481 agagcccgcg gctggatttg cgcacggttg ccgccgtcag tctcgtgcga gccgacgccg
      541 gcggacagac cgcggccgtc gagcagctgg ccgatgccgc cgacgccgcc gtgctgctcg
      601 tgaaacccta tcagaccttc cgcttcgtca agccgccctt ccgcctggcg ctcgccgcga
      661 gcagcatgcc gcagggcgcg accgccgaca tcaaagcggc gctccagctg gccgaacgcg
      721 gcaccacgct cgacgccgcc atcaccttcc gcccgcaggg ccagccgctg tatcaggttc
      781 ggctgtacct gcccgaggga ttttcgctcg accgcctggc cccgagcgac ctcgaatggg
      841 cgatcacgac cgacaacaac cgccagctgc tcaccgttta cctgctcgac ggccgcagcg
      901 accagttcac gctgacgctg ctggggaaaa tcgccgcgcc gccgcagccg ccagcaccgc
      961 cggctcccgc gccggcaacc ggtcagccgt cgcgaagtct gcctctgccg cggctggaaa
     1021 ttttggacgt gcagaagcag gagggcgaga tcgtgctggt gcccgatccg gacaccgacg
     1081 tgcggctcga ccaggtgacc aatgccgaca ccgcgccatt gaccggcggg cccggctgga
     1141 tgaaggggga gcagcagccg ctggccaagg cggtgctgcg atatcgcgtt cccagtcact
     1201 ccgccacgat cacgctgacg ccgcggacgc cgatcgtctc ggcccgctcg atcagcaacg
     1261 tgaaaatcac gccgcgatcg atcgaagaga ccattctgat cgacttccag atcgagcagg
     1321 cgggcatccg ccgcgtctcg ttcctcgtgc cgcagcacct ggccaaggcc cggctgaaga
     1381 cgctgctgct caagcagaag acgatcgaga ccgcgacggg cgctgccggc tcgccgcccg
     1441 cgggcatggt ccgcgtccgg ttggaactgc aggattacgt ccgcggccgg tattcagtgc
     1501 tgctggagca cgaccggctg ctgacctcgg gcaaacagcg gatcgatttg cccgtgattg
     1561 aaaccggccg caccgaccgc cggctggtgg cgatcgaaaa cgccggccgc gacgaagtgg
     1621 tgatcgggcc caacgacgcc gcgggcctgg agccgatcaa ccggcagcag caggcctggc
     1681 gcgacctggc cgcggtgctg ggcgagagcg tgacgcaggc ctactcggcc ctcgacaatg
     1741 ccccgcagcc gaacctctcg ttcaccacgg tcgagcggca gcaggcggag cggccgcggg
     1801 cccgcatcgg cctgggcacg acgctgctgg tggtcgatgc ctcgggcgcg tatcgcgcct
     1861 tggtgcaata ccgcctgacg aacgccgaag agcagttcct ggaagtcacg cttcccgcgg
     1921 gcgcgcggct gtggaccgta caggtcgccg gcgaaccggt gaagccggtg cagaccgtgc
     1981 cgccgctggc gcacgtggtc cgcattccgc tcgtcaaaac gggcgaaggc gagggggact
     2041 accttgtcga gttgaagtac ggcggcaagc tgccggcgat ccacggactg gcccccgtcg
     2101 attttccgct gatccgcgag acaagcgtcg gcgtcgagct gagccaggtg cgattgctcc
     2161 tgcccgaatc gcagcagtgg ttcaacttcg gcggcacgct gcggcgggtc ctcgaagagg
     2221 aggagctgca aaagggcttt cacgcgtatc tgagcgagcg gattcgcgag gccaccgagg
     2281 ccctgtcgag cgcgaacgac ttcacgaagg tccgggccgc cgccaacctc aagcagtcgc
     2341 ggcagatgtt cgactacaac cgccagtacc aggccgccaa caacctcaaa cagctcgacg
     2401 ccgatttgcg gaatgagacc ctgctcggcc gggccgagca gcaggcccag tccgaattgg
     2461 ctcagcagca gcgcggcgaa gaggccgaca accgttcgcg gctgaattcc gcctggagca
     2521 agcagggcgt gacgcggtcg aagaatatcg tcgacgacct gggcagcaac ttcgacggcg
     2581 cggcggcggg cgacgccggc aaaatgaagg gggccgaggt cttcaatcct gcgttcctcg
     2641 accagaacaa gctgcggcgc gaggaggaaa agggtgaaaa ggacgccgcc aaggacctgc
     2701 ccgccggcaa gcccggcgag tcgcaacccg aaggcaaggg gagcggccgc tatttccgcg
     2761 gcaacaacca gccgatggac gaaagcggcg gcggcaagca gggccagggg caggcggacc
     2821 agaaagcgcc cgatatcgcc gacaaaaaag agctcgacca gttgcagcag aagctccaac
     2881 aggaagtcga gggccgcgag cagacccggc gttcgtcagg cgaccgcgcc cagcaattgc
     2941 agcgctatca gcagaacctc gatttccaga acaacgccca gcagccgctg aataactacg
     3001 cgttgccgat gcagccgcag gccggcggcg cgacgccgca attgggtaac ctggatggtc
     3061 ggctgggtca gcctggagga ggtcaaggtg gcggacagtt tggaggctac ggcggggttc
     3121 caaactccgg cggtaatcga tccggcagtg gacaatcgag cgacggcggg ctcggcaatc
     3181 cgaatgggcc catgaatcag ccgcagccgg cgcaggatcc caatgccgcc ccggcagctc
     3241 cgcagccgcc gccgggtgac gcggaaggct acatgcagat cg
//
LOCUS       DASRJB010000464         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3510243, whole genome shotgun sequence.
ACCESSION   DASRJB010000464 DASRJB010000000
VERSION     DASRJB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3510243"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VFB80_12265"
     CDS             <1..1636
                     /locus_tag="VFB80_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34590.1"
                     /translation="LLDNTGAVERFRREFRAAARLAHPNVVTAFDADQAGNLHFLVME
                     FVEGLSFDRLVAQTGPLSVAQACHFIRQAAIGLVHVHEQGMIHRDIKPQNLMVMRKGQ
                     VKILDCGLARIVQAVAPEPAASGAASGPEATSAGMILGTPDYIAPEQVNDPRSADIRA
                     DIYSLGCTLYFLLAGRPPFPHGTITDKLVAQASAEPPPLSQFRSDVPPGLSRVLARML
                     AKDPRERYATPGEVARDLAPFAGERAGEQAAPSAGEPPPPPLVAPVAAEAPTTAEPFA
                     GLTLASTPPPGKPAASGSFPKIGPLAIVAAILACGVALAMMVLAFPLAGWLFHRSAAT
                     AEPVPNVRPSTLPATAPAALAVTPVGKLPRRVLFLLPKDALWPADYRDVRKNLPTDIE
                     VVAAGTTLEPVKFHTGANVPQQLVKADVLLKDVRAADYGAIVFAGYSSEEFAPGGAGG
                     EQARRLINECKQQGRLITSICRGQLALLEHGELRHKTVAYCFYCKEQYPSHELNLRPT
                     SVERDGQLITAGRDTDAKAFAETIAAALNESPGATP"
     gene            1729..1992
                     /locus_tag="VFB80_12270"
     CDS             1729..1992
                     /locus_tag="VFB80_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34591.1"
                     /translation="MNKEEILKKLQGGELSVEEATKLLNEADAPKKGLYCKVSEKGAI
                     SVYGLQRMPVTLYVEQWTRLLDFADNLKEFMKENDAKLKRKGQ"
     gene            complement(2136..>2337)
                     /locus_tag="VFB80_12275"
     CDS             complement(2136..>2337)
                     /locus_tag="VFB80_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZN34592.1"
                     /translation="PLLVEGPVTIVDAAGNRFVVPPEKPAVALCRCGQSKNKPFCDGS
                     HKTCSFMAAERAPAAGGGLPMA"
BASE COUNT          394 a          825 c          764 g          354 t
ORIGIN      
        1 gctgctcgac aacaccgggg cggtcgagcg gttccgccgc gagttccggg cggcggcccg
       61 cctggcgcac cccaacgtcg tgaccgcctt cgacgccgac caggccggca acctgcactt
      121 cctggtgatg gagttcgtcg aggggctgag cttcgaccgg ctcgtcgcgc agaccgggcc
      181 gctgtcggtc gcgcaggcct gccacttcat ccggcaggcg gccatcggtc tggtgcacgt
      241 gcacgaacaa gggatgatcc accgcgacat caagccgcag aatttgatgg tgatgcggaa
      301 gggccaggta aaaatactcg actgcgggct ggcgcggatc gtgcaggccg tcgcgcccga
      361 gcccgccgcc agcggcgccg cgagtggacc tgaggcgacc agcgcgggca tgatcctcgg
      421 cacgcccgac tacatcgcgc ccgagcaagt caacgatccc cgcagcgccg acatccgggc
      481 cgacatctat tccctcggct gcacgctcta tttcctgctc gcgggccggc cgccgtttcc
      541 gcacggcacg atcaccgata agctcgtggc tcaggcttcc gccgagccgc cgccgctgtc
      601 gcagttccgc agcgacgtgc cgcccggcct gtcgcgcgtg ctggcgcgga tgctggccaa
      661 ggacccgcgc gagcgctatg ccacgccggg cgaagtggcc cgcgacctgg cgcccttcgc
      721 gggcgagagg gcaggggagc aggccgcgcc ttctgccggc gagccgcccc cgccgccgct
      781 ggtcgcgcca gtggcggcgg aagcgccgac cacggctgaa ccttttgctg gactcacgct
      841 ggcctccact ccgccgcccg gcaagccggc cgcgagcggc agcttcccga aaatcgggcc
      901 gctggccatc gtcgcggcga ttctggcctg cggcgtcgcg ctggcgatga tggtcctggc
      961 gttccccctg gccggttggc tcttccatcg ctcggctgcg accgcggaac ctgtgcccaa
     1021 cgtccggccc agcactttgc ccgcgacggc tccggcagca ctcgccgtga cgccagtcgg
     1081 aaagcttccc cgccgcgtgt tgttcctgct gccgaaagac gcgctctggc cggccgacta
     1141 tcgcgacgtg cgaaagaacc tgccaacgga catcgaagtc gtcgctgcgg gcacgacgct
     1201 cgaacctgtc aaattccata cgggcgccaa cgtgccgcag cagctcgtga aggccgacgt
     1261 cctgctcaaa gacgtgcggg ccgccgatta cggcgcgatc gtcttcgccg gctactcctc
     1321 cgaagagttc gcgccgggcg gcgcgggcgg cgagcaggcc cgccggctga tcaacgagtg
     1381 caagcagcag ggccgcctga tcacgtcgat ctgccgcggc cagctggccc tgctggagca
     1441 cggcgagctg cgccacaaaa cggtggccta ctgcttctac tgcaaggagc aatacccgtc
     1501 ccatgagttg aatctgcgcc cgacgtcggt cgagcgcgac ggccagctca tcaccgccgg
     1561 ccgcgacacc gacgccaagg cctttgccga gacgatcgcc gccgccctga acgagtctcc
     1621 gggcgccaca ccgtagcgca gtcctctttc cacggtcgcc agattccgca ctcgccgagc
     1681 gtgcggtaca ttgccgctgc tgattcgact cgcagctgga gaccatcgat gaacaaggaa
     1741 gagatcctca aaaaactgca gggcggcgag ttgtcggtcg aagaagcgac gaaactgttg
     1801 aacgaggctg atgcgcccaa gaaaggcctg tactgcaagg tcagcgagaa gggcgcgatc
     1861 tcggtctatg gcctgcagcg gatgcccgta acgctgtacg tcgaacaatg gacccgcctc
     1921 ctcgattttg ccgacaactt gaaggagttc atgaaagaaa atgacgcgaa gctgaagcgg
     1981 aagggtcagt agcagtggtc agtagggtgc ggttccccgt ccgctgcgat gatatccaga
     2041 acgccgcggt tgccgggagg gaactcgaca ccgacgcgcg gccctttgcc gaggccattg
     2101 ccgccgccct ggacgaccct gcccgcgcgc cgtagctagg ccatcggcag gccgccgccc
     2161 gccgccggcg cacgctcggc cgccatgaag ctgcacgtct tgtgcgagcc gtcgcaaaag
     2221 ggcttgttct tcgattgccc gcagcggcag agggcgaccg ccggcttctc tggcggaacc
     2281 acgaaccggt tgccggccgc atcgacaatc gtgaccggac cttccaccag cagcgga
//
LOCUS       DASRJB010000465         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2667418, whole genome shotgun sequence.
ACCESSION   DASRJB010000465 DASRJB010000000
VERSION     DASRJB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667418"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFB80_12280"
     CDS             complement(<1..663)
                     /locus_tag="VFB80_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34593.1"
                     /translation="MPSITTHARAVAPLRQAPAVAALLALCLACSTHLRGEEPAKPDD
                     PVRAKRLELLSSRVQQFEIEVAGDAKRKLTPGKQPILRWSNPVRDNLNDGVIYLFFEG
                     VRPRAVVTVWADGPKGNLASGNLWREFVSLSGEPLVCRRDGTALWSPKTGGLVDQVLA
                     DAPAPAARPAQRLTQLRDLARRFEAVNYKMDVPNELRLLTQPLHRYAKEEGKTLDGAL
                     FAF"
     gene            809..3808
                     /locus_tag="VFB80_12285"
     CDS             809..3808
                     /locus_tag="VFB80_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN34594.1"
                     /translation="MVRSRLALIALVLAILGGSTAAADIDFLRDIQPIIARHCAKCHI
                     EKREGGLRLASRRDAFVAADSGEVFIKPGDSGGSELFQRITADDDTRMPPEGERLSAA
                     EVALMKRWIDEGARWPDDAVAPRHWSYVKPVRSPLPVVSHPDWPQTFVDSWILARLDR
                     EKLLSSPEAPRTALLRRLSLDLRGLPPSLDEAEAFLADDSPDAFERQADRYFASPAFG
                     ERWARHWLDLARYADSNGFQRDGFRDLWAFRDWVVRALNADLPFDQFTIEQIAGDLLP
                     DATLDQKIATGFHRCTTVNVEAGTDQEENRVNQVIDRVNTTGAVWLGTTLECAQCHNH
                     KYDPITQQEYYRLFAFFNNTAIETAFRGKNATAAIDFTGPMLEMPSEQVGQERQRLQK
                     QVAEIDALLAAAKAKLPPPTPEWEAELLTSAEKSGQWHVLAPAAFESAGKATGTILGD
                     QSILVSGPVPDKDTYTVTLKTALPEITGLKLETLTDASLPGGGPGRGDDQRPNFVLHK
                     FSVAVEINGEPGPAVKFKSAAADFSQQGYAVAGAIDDDPKTAWAIGQQFGKPHEATFL
                     LAEPIRGGEGAELVVKLEQNFGAGRTIGRLRLSALTGDSEAAVPPDVVAILKTAASER
                     SAEQQKRLADYRLSLDPEVQKLRSERETAAKALADLTAPQTLVMQEMPTPRTTNIFRR
                     GDFLHPLAEVKPQTPNFLHAAVEGPPDRLTLARWLTSAENPLVARVTVNRWWAELFGR
                     GIVSTPEDFGIQGELPTHPELLDALALELTDRAWSMKTILRRIVASATYRQSSKVTPE
                     LLARDDQNRLYARSPRLRLDAETVRDNAMAIAGLLSLKLGGPPVKPPQPEGVWTVTGL
                     VDNTYKVSPGEDAYRRGIYVVWRRSAPYPSFMNFDASIRAACVVRRSRSNTPLAALTL
                     LNDPVYVEAAAALARRIVAEAPSITDGSRIRHGFLLAVCREPTATEFSAVAKLLSEAR
                     ERHGPNEQAVWQELATVLLNLDETITK"
     gene            3956..>4390
                     /locus_tag="VFB80_12290"
     CDS             3956..>4390
                     /locus_tag="VFB80_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34595.1"
                     /translation="MTRRALLQSGGISLGAIALAGLLNEGRGIEPAATADPLAPRPSH
                     FAAKAKSVIYLHMVGAPSQLDLFDHKPELVKRDGQLCPQEFLDGQRFAFLRGHPKLMG
                     TQFKFARAGRGEIELSELLPELTKVADELAVIKTVHTEEFNHG"
BASE COUNT          809 a         1475 c         1371 g          735 t
ORIGIN      
        1 gaaagcgaac agcgcgccgt cgagagtttt cccctcttct ttggcgtatc ggtgcagcgg
       61 ctgggtcagc aggcgcagct cgttggggac gtccatctta tagttgaccg cctcgaagcg
      121 gcgggccagg tcgcgcagct gcgtcaatcg ctgcgccggc ctggcggcgg gagcgggcgc
      181 atcggccagc acttgatcga ccaggccgcc cgtctttggc gaccagagcg ctgtgccgtc
      241 ccgccggcag acgagcggct ctccgctgag cgagacgaat tcccgccaca ggttgccgct
      301 ggccagatta cccttggggc catcagccca aaccgtgacg acggcccgcg ggcgaacccc
      361 ctcgaagaac aggtagatga ccccgtcgtt gaggttgtcc cggaccggat tgctccaccg
      421 caggattggc tgcttgccag gcgtcagctt gcgcttcgca tcgccggcga cctcgatttc
      481 gaactgctgc acgcgcgaac ttaatagctc cagccgcttg gcgcgcacgg gatcatctgg
      541 cttagcgggt tcttcgccgc ggaggtgagt cgagcaggcg agacaaagcg ccaggagcgc
      601 ggcgactgcc ggcgcttgtc gcagcggagc aactgcccgg gcgtgagtcg tgatggacgg
      661 catgatgcct ccgcagctgg cggagtgaat tcagacatta tgtctatgac tgctcttgcg
      721 agcaagcggt ccggcgggca gaagtcggga gaccagagag tacaatgtcc ccctgacagc
      781 ccctgtcctg aaaatggatt tggcccacat ggttcgcagt cgtcttgcac ttattgcttt
      841 ggtgcttgcg atcctcggcg gctcgactgc cgccgccgac atcgacttcc tccgcgacat
      901 ccagccgatc atcgcccgcc actgcgcgaa gtgccacatc gagaagcggg aaggggggct
      961 gcggctggcc agccgtcggg acgcgttcgt tgcggccgat tcgggcgaag tgttcatcaa
     1021 gccgggagac agcggcggca gcgaactctt ccagcgaatc acggccgacg acgacacgcg
     1081 catgccgccg gaaggggagc ggctgagcgc ggcggaagtc gctctgatga agcgctggat
     1141 cgatgaaggg gccaggtggc cggatgatgc tgtagcgcca cggcactggt cgtacgtgaa
     1201 gccggttcgc tcgccgctgc cggtagtctc gcaccccgac tggccacaga cgttcgtcga
     1261 tagctggatc ctcgcccggc tcgatcgcga gaaactgctg tcgtcgcccg aagccccgcg
     1321 cacggcactt cttcgccgcc tgtcgcttga cctgcgtggg ctcccgccgt cactcgacga
     1381 agcggaggcg tttctcgccg acgattcgcc cgatgccttc gagcgccagg ccgatcgcta
     1441 ctttgcttca ccggctttcg gcgagcgctg ggcacgccac tggctcgacc tggcccgcta
     1501 cgcggactca aacggctttc agcgcgacgg ctttcgcgac ctgtgggcct tccgcgactg
     1561 ggtcgtgcgg gcgctgaatg cggatttgcc gtttgaccaa ttcacgatcg agcagattgc
     1621 cggcgatctg ctgccggatg ccacgctgga ccaaaaaatt gccaccggct ttcaccgctg
     1681 cacgacggtc aacgtcgaag ccggcaccga ccaggaggag aaccgcgtca atcaggtgat
     1741 cgatcgcgtg aacacgaccg gcgcggtctg gctcggcacg acgctggaat gtgcccagtg
     1801 ccacaaccac aagtacgatc cgatcacgca gcaggagtat taccggctgt ttgcattctt
     1861 taacaacacg gcaatcgaga ctgccttccg cggcaagaac gccacggcgg cgatcgactt
     1921 cacgggaccg atgttggaga tgcccagcga gcaggtcggt caggagcggc agcggctgca
     1981 aaagcaggtt gccgaaatcg atgcgctgct ggcggccgcc aaggcaaaac tgccgccgcc
     2041 aacgcccgag tgggaagcgg agctgctcac cagcgccgaa aaatcgggcc agtggcacgt
     2101 tctggcgcca gcggcgttcg aatcggctgg aaaggccacc ggcacgatcc ttggcgacca
     2161 gtcgatcctc gtcagcggcc cagtgccgga caaagacacc tacacggtca cgctgaagac
     2221 cgcgctgccg gagattaccg gtctaaagct cgaaacgctc accgacgcgt cgctgcccgg
     2281 cggcggccca ggccgcggcg acgaccagcg gccgaacttc gtgctccaca agttttcggt
     2341 cgccgtcgag attaacggcg agcccgggcc ggcggtaaag ttcaaatccg cggcggccga
     2401 cttttcgcag caggggtatg cggtggccgg cgcgatcgac gacgatccga aaacggcgtg
     2461 ggccattggc cagcaattcg gcaagcccca cgaggcgacg ttcctcctgg ccgagccgat
     2521 ccgcggcggc gaaggagcgg aactcgtcgt caagctcgag cagaacttcg gcgccggccg
     2581 caccatcggc cgcctgcgtc tctcggcact caccggcgat tctgaagcgg ccgtgccgcc
     2641 ggacgtcgtc gcgatcctga aaaccgcggc gagcgagcgg tcggccgagc agcagaaacg
     2701 gctggccgac taccgcctgt cactcgatcc ggaggtgcaa aagctccgca gcgagcgcga
     2761 aacggccgcc aaggcgctcg ccgacctgac cgccccgcag acgctcgtga tgcaggaaat
     2821 gccgacgccg cggacgacca acatcttccg ccgcggcgat ttcctccatc cgctggccga
     2881 ggtcaagccg cagactccaa actttttaca tgcggccgtg gagggcccac ccgatcgcct
     2941 gacgctggcc cgctggctaa ctagcgccga aaacccgctc gtcgcccgcg tcaccgtcaa
     3001 ccgctggtgg gccgaactct tcggccgcgg cattgtcagt acgcccgaag atttcggcat
     3061 tcaaggggag ctgccgacgc atccggagtt actcgacgcg ctggccctcg agctaaccga
     3121 tcgcgcctgg tcgatgaaaa ccatcctccg ccgcatcgtt gcctccgcca cctatcgcca
     3181 gtcgtcgaaa gtcacgcccg agctgctggc ccgcgacgac cagaaccggc tctacgctcg
     3241 cagcccaagg ctgcggctcg atgccgagac cgtgcgcgac aacgccatgg cgattgccgg
     3301 gctcctttcg ctcaaattag gcggcccgcc ggtcaagccg ccgcagccgg aaggcgtctg
     3361 gaccgtcacg ggcctcgtgg acaacacgta taaggtctct cccggggagg acgcctaccg
     3421 ccgcggcatt tacgtcgtct ggcgacgcag cgccccgtat cccagcttca tgaacttcga
     3481 cgcgtcgatc cgcgctgcct gcgtcgtgcg gcgctcgcgc tcgaacactc cgctggcagc
     3541 cctgacgctc ctcaacgacc cggtctacgt cgaagcggcc gctgcactgg cccgccggat
     3601 cgtcgctgaa gccccctcca tcaccgacgg gagccgaatt cgccacggat ttttgctggc
     3661 cgtctgccgc gagccgacgg ctaccgagtt ttcggccgtt gccaagctgc tcagcgaagc
     3721 gcgcgagcga cacggaccaa acgaacaagc cgtatggcag gaactggcga ccgtgctact
     3781 gaatttggat gaaaccatta ccaagtaaac aaacagtcag aattgattcg ccgcggattt
     3841 cgcggatgac gcggatcagg cacgaaaaat ccgcgtcatc tgcgttatcc gcggccaaac
     3901 ttgcgatgcg aactgattga ggtagcacgt ggattcttca tcgcgatttg tttcattgac
     3961 ccgccgcgct cttctccaat ccggcggcat ctcgctgggc gccatcgctc tggccggtct
     4021 gctcaatgaa ggccgcggca tagagccagc cgccactgct gatccactcg ctccgcggcc
     4081 ctcgcacttt gccgccaagg cgaaaagcgt catctatctc cacatggtcg gcgcgccgag
     4141 ccaacttgac ctgttcgatc acaagcctga gctggtgaag cgagacggcc agctctgccc
     4201 gcaggagttc ctcgacggcc agcggttcgc cttcctccgc ggccatccta agctaatggg
     4261 gacgcagttc aagttcgccc gcgccggccg tggcgaaatc gagctgtcag aattgctccc
     4321 cgagctgacc aaagtcgccg acgagctggc agtgatcaag accgtccaca ctgaggagtt
     4381 caaccacggc
//
LOCUS       DASRJB010000466         5580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2951272, whole genome shotgun sequence.
ACCESSION   DASRJB010000466 DASRJB010000000
VERSION     DASRJB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951272"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFB80_12295"
     CDS             complement(<1..605)
                     /locus_tag="VFB80_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34596.1"
                     /translation="MNPIPDLPPVERRHFLRRAALAPLCLLLVAGLHAARVWTARQTP
                     WKGGGFGMFSTIDSEGNRFLRAYLITGSGELPLPLPAALDKPAAEQRAAPSVVGLKSI
                     ARRLAAQDWRWTDDRQRRQAQAISQRDGLEISAAALRCPSSVTAAPLSAGQSHALEPL
                     PRSELPGETIPFTAVRLECWRGRFDAATDLLSAERLLSVTVT"
     gene            855..1859
                     /locus_tag="VFB80_12300"
     CDS             855..1859
                     /locus_tag="VFB80_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34597.1"
                     /translation="MSHRRTWGKGFTVVEMLVVISIIGVMMALLLPAVQAARETARRM
                     QCSSNLRQLGIAVVNFDTTKQHLPPARSFPVLSPTVYAKPQNWNQTGAFPEQVVSWVY
                     FILPNIEQGDLKTTMDKQFLQAGIDITTMHQSAPIAVRPLHCPSDQTDRAPMHVSYAC
                     NGGLSDDTSPANNNPFDWAANGVFLTRLKGTNDTHKIEQQSLGDIASADGNSNTIMLA
                     ENHYLDLWHNATSEYSASIVWQESPTSIGGQLGIDLTMGYGPMLDEDHAHPSSLHPGG
                     FNVVMCDGSTKYIADSVDYTVYARLMTSNGKKYKSAGQSTAIGAVQTIQQTPLSADSY
                     "
     gene            1999..4272
                     /locus_tag="VFB80_12305"
     CDS             1999..4272
                     /locus_tag="VFB80_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN34598.1"
                     /translation="MWPSSAWLIAPALVALLAGPLAGQTLSHSVPGAVAPGRTTEIVL
                     HGAKLGPGSRVWTSFPAQLEIAADPNAKEPAQLACKITLPAGSGAGIGGIAVATPAGL
                     SEVLYMMIDDLPSTAEVATNHTAGSPQDVPLPAAIDGASDGTVLDYFRFTAKAGQRIA
                     CEVVASRLGWDYDAVCRVLDASGAELLRADDDPSSGADARFVFTAPADGQYVLEVGDN
                     RYKPGGRYRLRLGDFPLVSTPLPLVASAGAATPIAFAGPHVDGLAAATLLAPLAAAGQ
                     SLPLSVHAAGGQASGWTTLGVTDLPVVVESSGTASIPGVFCGQLAAAGEQDLFEFRAS
                     KGAAINFRGIGRSAGSAAILRLRVLNAAGNQVAESPVTDSDEPVLRFTAGEDGAFKLA
                     VSELAGRGGVDYTYAIEATTGPQFSLLLKNDKNNKAKHSLPEEGGALYLDVQCQRSGY
                     DGPIRLAVDSPRPGWQAFNAVIPAKANEVRMYLASPIDWSPGEFAPLRIVGTGDANVR
                     ATMETTTQLRTARPQMPYPPAWHDGTIFVSATESKPAFYSVAAAKSEVNFPRLVGQAQ
                     LTLDFARLDDKFKDVPLTVLPLGLPAGITAEVKRNGNGPKETYDITFKGPKDQAERQH
                     TVRYFAFAEYAGAGRAILSGDIRLNVITPLAVAAAPAGPLVQGQKQKVKLTLTRRGDD
                     KQPVDLKFKSLPAGVTAPEKTTLAADQNEVEVELTAAADAAIAKFDQLIAVASCKYAG
                     TDITIESPAASLEVKAP"
     gene            4286..>5580
                     /locus_tag="VFB80_12310"
     CDS             4286..>5580
                     /locus_tag="VFB80_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34599.1"
                     /translation="MKFTGRLLSSVALTVLVATAIQAADEPALQPIAAGQPVRLEVFP
                     KEIKLTGSRSRRQLVVTGHYEGGGVQDLTWASQFASSNPQIADVANAVVLPRQDGAAE
                     ITVTCGGQTAKVSVEVSAFTQPQPVSFEFDALAAISKQGCNSGACHGSPSGKGGFRLS
                     LRAFDPVLDKLTLIREDLGRRTNPLNPDESLLLNKPLMKVPHGGGLKLTKHDPAYAAL
                     RQWIAEGCRPDGDNVARCVRIELHPASGRLLKWPAHTQQLAVLAHFADGQVRDITELA
                     CYSTSDSQVADVTAAGRVIGKDRGEATIIVRYLEHIESCSLTFVKDVPNYVWNNPPAA
                     NYIDELVAAKLRQLQYQPAGLCSDEEFLRRVYLDVIGQLPTLAETKEFLADTAENKRA
                     KLIDRLLERPEHARFFALKWGDLLRLTSNQIGNSGVFKY"
BASE COUNT          984 a         1903 c         1838 g          855 t
ORIGIN      
        1 gtcacggtaa cgctcagcag ccgctcggcg ctgagcaagt cggtcgcggc gtcgaaccgc
       61 ccgcgccagc actcgaggcg gacggcggtg aaagggatgg tttcgcccgg cagctcgctc
      121 cgcgggagcg gttccagagc gtgcgactgg ccggcggaga gcggggctgc ggtcacggaa
      181 ctcggacaac ggagggccgc cgcggagatc tctagcccat cgcgctggct gatcgcttgg
      241 gcctgccgcc gctggcggtc gtcggtccag cgccagtcct gggccgcgag ccggcgggcg
      301 atggacttta gtcccaccac cgagggcgcg gcccgctgct cggccgcggg cttatcgagg
      361 gccgcgggca acgggagcgg caattcgccc gagcccgtaa tcaggtaagc gcgcagaaag
      421 cggtttcctt ccgagtcgat cgtcgagaac atcccgaagc cgccgccctt ccagggcgtc
      481 tgccgcgcgg tccagacccg cgcggcgtga aggcccgcca cgagcagcag gcagagcggg
      541 gccagcgccg cgcggcggag aaaatgccgc cgctcgacgg gcggcaaatc gggaatgggg
      601 ttcattcaca aatctacgtc ctggtcgttt aacccggagc cgaaggcgac ggcgtattcc
      661 ggtagccccc gtcgcctccg gctccgggtt aaacgaggcg gcataatcgt cctcgcccct
      721 acattcgtca aaaaactcgc cttccgactc gacatccgct ccgccaagcg ccacaatcag
      781 ggcagaatct ggcgcgggcg ttcgcccggc gcatcgctac ggggaacaga atcagagaga
      841 aagagaggcc agctatgtcg caccgtcgta cttggggcaa agggtttacg gtcgtcgaga
      901 tgctcgtcgt catctcgatc attggcgtga tgatggccct gctgttgcct gccgtgcagg
      961 ccgcccgcga aaccgcgcgg cggatgcaat gcagttccaa tctccggcaa ctcggaattg
     1021 ctgtggtgaa cttcgacacc acgaagcagc acctgccgcc tgcgaggtcg ttccccgtgc
     1081 tgtcgccgac ggtctatgcg aagccccaga actggaacca gacgggtgcg ttccccgagc
     1141 aggtcgtgag ctgggtctat ttcattctgc ccaacatcga gcagggcgac ctgaagacaa
     1201 cgatggacaa gcagtttctc caggcgggta tcgacatcac cacgatgcat cagagcgctc
     1261 cgatcgccgt ccgcccgctc cattgcccgt cggatcagac cgaccgcgcg cctatgcacg
     1321 tcagctatgc ctgcaacggc gggctgtccg acgacacctc gcccgccaac aacaatccgt
     1381 tcgattgggc cgccaacggc gtgtttctga ctcgcctgaa gggcaccaac gatacgcaca
     1441 aaatcgagca gcagagcctc ggcgacattg ccagcgccga cggcaacagc aacacgatca
     1501 tgctcgccga gaaccattac ctggatttgt ggcacaacgc cacgagcgag tactcggcgt
     1561 cgatcgtctg gcaggagtcg cccacgtcga tcggcggcca actcggcatc gacctcacca
     1621 tgggttacgg gccgatgctc gacgaggacc acgcccatcc ctcgagcctg caccccggcg
     1681 gcttcaacgt cgtgatgtgc gacggcagca cgaagtacat cgcggattcg gtggactaca
     1741 cggtctacgc gcggctgatg acctccaatg gcaagaagta caagagcgcc gggcagagca
     1801 cggccatcgg cgcggtgcag acgatccagc agacgcccct gagcgcggat tcctactagg
     1861 cgacttcgtc cggcgtgcgg ttggttgacc tttacggccc tgccatccac aatggggacg
     1921 catggcgggg ctgaagctgc cgccacgcgc cgctcgtgcg gccctgcctg aaaacctccc
     1981 gcctggacga cgacttcgat gtggccatct tctgcttggc tgattgcccc tgcgctggtg
     2041 gccctgctcg ccgggccgct ggccggccag acgctgagcc actccgtccc cggggcggtc
     2101 gcgccgggtc ggacgactga gatcgtgctg cacggcgcga agctgggacc tggctcgcgg
     2161 gtctggacca gttttcccgc ccagctcgag atcgcggccg atcccaatgc caaggaaccg
     2221 gcgcaactgg cttgcaagat cacgctgccg gcaggcagcg gcgctgggat cggcgggatc
     2281 gccgtggcca cgcccgccgg cctctctgaa gtgctctaca tgatgatcga cgacctgccg
     2341 agtacggccg aagtcgccac caaccacacg gccggttcac cgcaggacgt gccgctgccc
     2401 gcggccatcg atggcgcgag cgatggcacc gtgctcgact atttccgctt cacggccaag
     2461 gccggccagc ggattgcctg cgaagtcgtg gcctcccgac tgggctggga ttatgacgcg
     2521 gtctgccgcg tcctcgacgc gagcggcgcg gaattgctgc gggccgacga cgaccccagc
     2581 agcggggccg acgcgcggtt cgttttcacc gcccccgccg acgggcaata cgtgctggag
     2641 gtcggcgaca accgctacaa gcccggcggt cggtatcgcc tgcgtctggg cgactttccg
     2701 ctcgtcagca cgccgctgcc gctggtcgcc tccgccggcg cggcgactcc gatcgcgttt
     2761 gccgggccgc acgtcgatgg gctcgctgcc gccacgctgc tcgcgccgct ggctgccgcc
     2821 gggcaatcgc tgccgctctc ggtccacgcc gcaggcggcc aggcctccgg ctggacgaca
     2881 ctcggcgtga ccgacttgcc ggtcgtcgtt gaatcgtcgg ggaccgcgag cattccaggc
     2941 gtcttttgcg ggcagctggc cgccgcgggc gagcaggatc tgtttgagtt ccgcgcgagc
     3001 aagggcgcgg cgatcaactt ccgcggcatc ggccgcagcg ccggctcggc ggcgatcctg
     3061 cggctgcgcg tcctgaacgc cgctgggaac caggtcgccg agagtccggt gaccgacagc
     3121 gacgaaccgg tcctccgctt cacggccggc gaggacggtg cgttcaagct ggcggtgagc
     3181 gagctggccg gccgcggcgg cgtcgattac acgtacgcca tcgaagccac gaccggcccg
     3241 cagttttcgc tgctcctgaa gaacgacaag aacaataagg cgaagcactc gctgcccgag
     3301 gagggcgggg ctctctatct cgacgtccag tgccagcgct ccggctacga cgggccgatt
     3361 cgcctcgccg tcgattcgcc tcggcccggc tggcaggcgt tcaatgccgt gatcccggcc
     3421 aaggccaacg aagtgcgaat gtacctggcc agccccatcg actggtcgcc gggcgaattt
     3481 gcgccgctgc gaatcgtcgg cacaggcgac gccaacgtgc gggccacgat ggaaacgacg
     3541 acgcagctcc gcacggcccg gccgcagatg ccctatccgc ccgcgtggca cgatggaaca
     3601 atcttcgttt cggccacgga atcgaagccc gcgttctatt cggtggcggc cgccaagagc
     3661 gaagtgaact tcccgcggct cgtcggccag gcccagctca cgctcgattt cgcccgcctc
     3721 gacgacaaat tcaaggacgt cccgctgacc gtgctgccgc tgggcttgcc ggctggaatc
     3781 accgccgagg tcaagcgcaa cggcaacggc ccgaaggaga cgtacgacat cacgttcaag
     3841 ggtccaaagg atcaggcgga gaggcagcac acggtccgct acttcgcctt cgccgaatac
     3901 gcgggcgcgg gtcgcgcgat cctctccggc gacatccggc taaacgtgat cacgccgctg
     3961 gcggtggccg ccgccccggc cgggccgctc gtgcagggcc agaagcagaa ggtgaagctg
     4021 acgctcactc gccgcggcga cgacaagcag cccgtggacc tgaaattcaa gtcgctcccc
     4081 gccggcgtga ccgcgcccga gaagacgacg ctcgccgccg atcagaacga agtcgaggtc
     4141 gagctgacgg ccgctgctga tgcggcaatc gccaaattcg accagctgat cgccgtcgcg
     4201 agctgcaagt acgccggcac ggacatcacg atcgaaagcc ccgccgccag cctcgaagtc
     4261 aaggcgccgt aaggaggata cccctatgaa gttcaccgga cgactgcttt cgtcggtcgc
     4321 gctgacggtc ttggtggcca ctgcgattca ggccgcagac gagcccgcgt tgcagccgat
     4381 cgccgccggt cagccggtgc gcctcgaagt gtttcccaag gagatcaagc tcacaggttc
     4441 acgcagccgg cggcagctgg tggtgacggg ccactacgag ggtggcggcg tgcaggatct
     4501 gacctgggcc agccagttcg ccagcagcaa tccgcagatc gccgacgtcg ccaatgccgt
     4561 ggtcctcccg cggcaggacg gagcggcgga aatcaccgtg acttgcggcg gtcagaccgc
     4621 gaaagtgtcg gtagaagtga gcgccttcac gcagccgcag cccgtgtcgt tcgagttcga
     4681 tgccctggcc gcgatcagca agcagggctg caatagcggg gcgtgccacg gctcgccgag
     4741 cggcaagggc ggcttccgcc tctcgctgcg ggcgttcgac ccggtgctcg acaagctgac
     4801 gctgatccgc gaggacctcg gccggcggac caacccgctg aatccggacg agagcctgct
     4861 gctgaacaag ccgctgatga aagtgcctca cggcggcggc ctgaagctga cgaagcacga
     4921 cccggcctat gccgcgctgc ggcagtggat tgccgagggc tgccggccgg atggggacaa
     4981 cgtcgcgcgg tgcgtgcgga tcgagctgca ccccgccagc ggccgactgc tcaagtggcc
     5041 ggcccacacc cagcaactcg cggtgctggc tcatttcgcc gatgggcagg tccgcgacat
     5101 caccgagctg gcctgctatt ccacttccga cagccaggtc gccgacgtga ccgcggccgg
     5161 ccgcgtgatc ggcaaggacc gcggcgaggc gacgatcatc gtccgctacc tggagcacat
     5221 cgagtcctgc tcgctgacgt tcgtcaagga cgtcccgaac tacgtctgga acaatccgcc
     5281 ggcggcgaac tacatcgacg agctggtcgc ggccaagctg cggcagcttc agtatcagcc
     5341 cgccggcctg tgcagcgacg aggaattcct gcggagggtt tatctcgacg tgatcggcca
     5401 gctgcccacg ctggccgaga ccaaggagtt cctcgcggac acagcggaga acaagcgggc
     5461 gaaactgatc gaccggctgc tcgagcggcc tgagcacgcc cgattctttg cgctcaagtg
     5521 gggcgaccta ctcaggctga cgagcaatca gatcggcaac agcggcgtat tcaagtatca
//
LOCUS       DASRJB010000467         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4078043, whole genome shotgun sequence.
ACCESSION   DASRJB010000467 DASRJB010000000
VERSION     DASRJB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078043"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VFB80_12315"
     CDS             <1..1867
                     /locus_tag="VFB80_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN34600.1"
                     /translation="AANFFLTDGQQNNSGFLAPGTYSVAEVNIPSGWTLSSAVCSDGS
                     PANAISLQPGETVTCIFTNTQQGQIIVQKQTLPDGSAQSFEFDPSYAANFFLTDGQQN
                     NSGFLAPGIYSVAEVNIPSGWTLSSAVCSDGSPVSAVSLQPGEIVTCVFTNTQKNEII
                     VIGPDKSPTVPAPVLIVDKDTGAIVNSFLAYEPTFVGGTRVATGDLTGDGIDEIITAP
                     GRGRAPEVRVFTQAGVELPQFRTLAYAATYVGGVHVAVGDVNGDGLNDIITSPSSGKV
                     LVKVFLNQIPNVDPIANVPFKSFYAFPTTFVGGGFVRAADMGLRLGSGTFVNTLDGKA
                     EIIVGSGPGIKAAVKVFHVNGTPTAVRTVFPFSTASSVYKNGVNLDVARLNADLIPDI
                     IVGTENGGNSRVETFVWNGSAALVKTGSFMAYGDSPSKIAPVRVAALDASGDGIADKL
                     ATVQGPGGTTQQIRCFDIVSPNPLSVTQTAALGGFPGPYWIAALDSPAPPPQPIEAEG
                     EADFEASALSVTSAPNLDVNGDGAVTAQDALLVVNGLNQQSAGTSLTTEQVKALDVSG
                     DGQLTALDALLLVNELNRTAAAVAALAPAISSATSSSQSDESRFTAEVDELFALL"
BASE COUNT          390 a          753 c          640 g          348 t
ORIGIN      
        1 cgccgccaac ttcttcctga ccgacggcca gcaaaacaac agcggctttt tggcgccggg
       61 aacctattcc gtcgccgaag tcaacatccc cagcggctgg acgctcagca gtgccgtctg
      121 ctcggacggc agcccggcca atgccatctc gctccagccg ggcgagaccg tcacctgcat
      181 cttcacgaac acgcagcagg gccagatcat cgtccagaaa cagacgctac ccgacggcag
      241 cgcccagtcg tttgagttcg accccagcta cgccgccaac ttcttcctga ccgacggcca
      301 gcagaacaac agcggcttcc tggctcctgg catttactca gtggccgaag tcaacatccc
      361 cagcggctgg acgctcagca gtgccgtctg ctcggacggc agcccggtaa gcgccgtctc
      421 gctccagccc ggcgagatcg tcacctgcgt cttcaccaac acgcagaaaa acgagatcat
      481 cgtcatcggc cccgacaaga gcccgaccgt gccggccccg gtgctcatcg tcgacaagga
      541 caccggcgcg atcgtcaact cgttcctcgc ctacgagccg accttcgtgg gcggcacgcg
      601 cgtcgccacc ggcgatctga ccggtgacgg catcgacgag atcattacgg cccccggccg
      661 cggccgcgcc cccgaggtcc gcgtcttcac gcaggcggga gtcgagctgc cgcagttccg
      721 cacgctggcc tatgccgcca cgtacgttgg cggcgttcac gtggcggtcg gcgacgtcaa
      781 cggcgacggt ttgaatgaca tcatcacctc cccctccagc ggcaaggtcc tggtgaaggt
      841 cttcctgaac cagattccca acgtcgaccc gatcgccaat gtgccgttca agtcgttcta
      901 cgctttcccg accaccttcg tcgggggcgg tttcgtgcgg gccgccgaca tgggcttgcg
      961 gctgggcagc ggcacgttcg tgaatactct cgacggcaag gccgagatca tcgtcggcag
     1021 cggcccgggg atcaaagcgg ctgtcaaggt gttccacgtc aatggcacgc cgacggccgt
     1081 ccgcaccgtg ttccccttca gcacggccag ctcggtctac aaaaacggcg tcaacctgga
     1141 cgtggcccgc ctcaacgccg acttgatccc cgacatcatc gtcggcacgg aaaacggcgg
     1201 caattcgcgc gttgaaacgt tcgtctggaa cggcagcgcg gcactggtaa aaaccggctc
     1261 gttcatggcc tacggcgaca gccccagcaa gatcgccccg gtccgcgtcg ccgcgctcga
     1321 cgccagcggc gacggcattg ccgacaagct ggctacggtg cagggtccgg gcggcaccac
     1381 ccagcagatt cgctgcttcg acatcgtcag ccccaacccg ctgtcggtca cgcagaccgc
     1441 cgccctcggc ggattcccag gaccatactg gatcgcggca ctcgacagcc cggcgccgcc
     1501 gccgcagccc atcgaggcgg aaggagaagc tgacttcgag gcttcggcac tcagcgtgac
     1561 atccgccccg aacctcgacg tcaacggcga cggcgcggtg accgcgcagg acgcgctact
     1621 ggtggtcaat gggctcaacc agcagtccgc gggcacttcg cttacgaccg agcaggtgaa
     1681 ggcgctcgac gtcagcggcg atggccagct cacggcactc gacgcgctgc tgctggttaa
     1741 tgagctgaac cgcactgcgg ccgccgtggc ggcactggcc ccggccattt ccagcgcgac
     1801 gagcagcagc cagtcggacg aaagccgctt cacggcggag gtggacgagc tgttcgcgct
     1861 gctgtagagc actcgctccg cgagtgctca atcaacttgt tcccaggctc cgcctgggaa
     1921 cacactaacc ccaggctcct gcctcggcgg gccgcgtcaa ttcgactttt ctttgcgaag
     1981 tggcggctcc acttggaacc acgagccgtc cgcggagtag cggcgccatg tgtcgccagt
     2041 gtcggaatcg aacagaacct gtgagtcgaa gttcttcgga tcgatatgca attcgtagcg
     2101 cccgattgcg ggattcgacc gaggggcgtt c
//
LOCUS       DASRJB010000468         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3511311, whole genome shotgun sequence.
ACCESSION   DASRJB010000468 DASRJB010000000
VERSION     DASRJB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511311"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFB80_12320"
     CDS             complement(<1..809)
                     /locus_tag="VFB80_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34601.1"
                     /translation="MHVGFISVAAGLALVAVGCGHEPTAAIPPPAFQPQQLPAKHLPN
                     PWQIHPQVISGGLPEGEAAFGELAALGVKTVISVDGARPDVALARKYGLRYVHLPHGY
                     DGIPAQRMKELAKAVRDLPGPIYIHCHHGHHRSPVAATVACVGAGLLTHDHALAVLRT
                     AGTSENYRGLYQSAELARRLDDALLDELKAEYPETAQIPPIADAMIAIEHTHDHLKAL
                     AANRWRTLEKQPDLGAAHEALLLREHYTELARTDEINSQPAGFRKLLAESEA"
     gene            complement(895..>2208)
                     /locus_tag="VFB80_12325"
     CDS             complement(895..>2208)
                     /locus_tag="VFB80_12325"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34602.1"
                     /translation="AAVRALQLIKPDRNVSIPLVVKLLQDSDPNVAMLALSAIAEGGP
                     EAVPVLTEALEHPEARYWACLALSEIGAAAKPAVSGLIKALADERPEVRLQAAIALAE
                     IGPDAKPAVPTLVKLLADPFESLRHASAFALGRIGDKSAAQALGALQTSSDTLLQSLS
                     IWALAKMYPEDKPRQEAAVGHLVLRLADKNPSIGHMAARAIAELSPADEVIRPPMDKL
                     LASADAETAERIFTAYASLGPKIVPLAIRALKDTPQRRERALQVLSRMGADAAPALPE
                     LIEIAKSGPAKEKVEALFIIGSIGPKADSAVSAVIAALGDADPVVKSAASYAVGKIGP
                     AAKDALPALRTMAGADDEMLRLSSVWAMLQIGAMNDEVTKMAVPMLAKALTNEREFVR
                     VEAAMSLGKLGKAATSAVPALEKAQQDPSPAVRHAAAEALKLIKG"
BASE COUNT          319 a          735 c          769 g          385 t
ORIGIN      
        1 gcttcgctct cggccagcag tttccggaat ccggcgggct ggctgttgat ctcgtcggta
       61 cgagccagct cggtgtagtg ttcgcgcaac aggagcgcct cgtgagccgc gcccagatcc
      121 ggctgcttct caagcgtccg ccaccgattg gctgccaggg ccttcaagtg gtcgtgcgta
      181 tgctcgatcg cgatcatcgc atcggcaatg ggcggaatct gggccgtctc gggatactcc
      241 gccttcaatt cgtcgagcag tgcgtcgtcc aatcgccgcg ccaactccgc cgattgatac
      301 agcccgcggt aattctcgct ggtgccggcc gtcctcagga ccgccagcgc gtgatcgtgc
      361 gtcagcagcc ccgcgccgac gcaggcgacc gtcgccgcga ccgggctgcg atgatggccg
      421 tgatggcaat gaatgtagat cggccccggc aggtcgcgga ccgccttggc cagctctttc
      481 atccgctggg ccgggatacc gtcgtagccg tgcggcaagt gaacgtagcg caggccgtat
      541 ttcctggcca aggccacgtc gggccgagcg ccatcgacgc tgatgaccgt cttcacgccc
      601 agggcagcca gctctccaaa cgccgcctcc ccctcgggca agccgcccga gatcacctgg
      661 ggatgaatct gccacgggtt cggcagatgc ttcgccggca gctgctgtgg ctggaaggca
      721 gggggcggaa ttgccgctgt cggttcgtgg ccgcacccga cggcgacgag cgccagcccg
      781 gctgcgacgg agataaagcc gacgtgcatt cccgcatccg tctcttggat tactatgggc
      841 ctgtctgctg gtcgcgcgcc gctaagggat tcgctccgcg gccgggaata ttggttaccc
      901 cttgatcagc ttaagggctt cggcggcggc gtggcggacg gcggggctgg gatcctgctg
      961 ggccttttcc agggcgggaa cggcggatgt cgccgccttg cccagcttgc ccagcgacat
     1021 ggcggcctcg acgcggacga actcgcgctc gttggtcagg gccttggcca gcatcggcac
     1081 cgccatcttt gtcacttcgt cgttcatggc gccgatctgg agcatcgccc agacgctgct
     1141 gagcctgagc atctcgtcgt ccgcgccggc catcgtccgc agggccggca gggcgtcctt
     1201 ggcagctggg ccgatcttgc ccacggcgta actggcagcc gacttcacca ccgggtcggc
     1261 gtcgcccagc gcggcgatga cggcggaaac cgcggagtcg gctttcgggc cgatcgaccc
     1321 gatgatgaac agggcctcga ccttttcctt ggccgggccg gacttggcga tctcgatcag
     1381 ttccggcagc gcgggcgcgg cgtcggcacc cattcgggaa agcacctgca gggcccgttc
     1441 gcggcgctgg ggcgtgtctt tgagcgcccg gatcgccagc ggcacgatct tgggaccaag
     1501 cgacgcgtag gcggtgaaaa tccgctcggc ggtctccgcg tcggcgctgg cgaggagttt
     1561 gtccatcggg ggccggatga cttcgtccgc gggcgagagc tcggcgatcg cgcgggcggc
     1621 catgtggccg atgctcgggt tcttgtcggc cagccgcagg accaggtggc cgacggcggc
     1681 ctcttgtcgc ggcttgtctt cgggatacat cttggccagg gcccagatgc tcagcgattg
     1741 cagcagcgtg tcagacgagg tttgcagcgc tcccagcgcc tgggccgcgg acttgtcgcc
     1801 gatccgaccc aaggcgaacg ccgaggcgtg ccgcagcgac tcgaacggat cggccagcag
     1861 cttaacgagc gtcggcaccg cgggcttggc atcggggccg atctcggcca gggcgatcgc
     1921 cgcctgcagc cgcacttcgg gccgttcgtc ggccagggcc ttgatcagcc cggaaaccgc
     1981 gggcttggcc gcggcgccga tctcggagag cgcgaggcag gcccagtagc gggcttcggg
     2041 atgctcgagg gcttcggtga gcaccggcac ggcttccggg ccgccctcgg cgatggcact
     2101 gagcgccagc atcgccacgt tcggatcgga atcctggagc agcttcacca ccagcggaat
     2161 cgacacgttg cggtccggct tgatcaattg cagcgcgcgg acggcggc
//
LOCUS       DASRJB010000469         7853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3511429, whole genome shotgun sequence.
ACCESSION   DASRJB010000469 DASRJB010000000
VERSION     DASRJB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511429"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFB80_12330"
     CDS             <1..1341
                     /locus_tag="VFB80_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34603.1"
                     /translation="EVLVIDGLRPERGDAQLWRQDTIEPDQSSRQATIYTQQRTTNNP
                     LLGPRLINYDATNRMNFNSGPLLTGGFCFQRGRTLFCVDPLTGQSLWERALPWPQAEI
                     FGDDELLFVSDATGQQTLMLSAIDGSLLSRRKLEPAERRWATCGRNVLAFEAAGTTIQ
                     LRLYDASRQPDPTEPAAAAGEALWTRTVPLGTRGCTIDGEAIGLLEPGGQFTVVSLAS
                     GQVRLAVPLEPEPSLSWIQVQTSRDQLVLLASQEQGDPTGNVMMQPLQTVNNPLGRMH
                     GRVYAFQRKTGKLQWQTAAFISQHGMPPDQPAESPLLFFVRNRTEAGRGNTRSTTSVL
                     ALDRRDGRPVYENDSVLPQIANNCEVSVEPAKQSVTLSLYGGANKTLVFKLSDKPTPP
                     QPPAQTGSLASSTAGQAPGIVDGTVGAAVELLNRGLNPRILLPRAAPPPPPARP"
     gene            complement(1347..1682)
                     /locus_tag="VFB80_12335"
     CDS             complement(1347..1682)
                     /locus_tag="VFB80_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34604.1"
                     /translation="MFGKPQWFRPKTIGFGVVPISWQGWLYTSGWLAAVGMPFLLLLS
                     RWQPLEAGAWLVLSLGALSYDVWQIRRALLAPVASAPPKTDDNVLYILDSQPGQQAAT
                     RNYNLQVRR"
     gene            2010..2756
                     /locus_tag="VFB80_12340"
     CDS             2010..2756
                     /locus_tag="VFB80_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34605.1"
                     /translation="MPATVLAQYAEPAEHSIIRQTGFGWRNHVEVADGCGCSVPVRAD
                     CYDQPCCFRCGLHPCCFLQRVHRMLDCLLPCKKCCCLLGGRCGGCCGSSYDDGVGGLP
                     GLSDPFVDDALVPPKPIADPGTEVRFQPAAPRRLPSLPPRTIVNKPAPVSRPAATTQP
                     RPSPYKVTAPPAAQPVEYRVSHARQAAASPAQPHLVRPAQQSILRRISADEPAPFEVE
                     ASSLQPIVRSQSPPAEAADDAIPHNPLRSR"
     gene            complement(2763..4025)
                     /locus_tag="VFB80_12345"
     CDS             complement(2763..4025)
                     /locus_tag="VFB80_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN34606.1"
                     /translation="MKLSRRSFLRAGGVSLALPLFDAVLDRQSRGALNADPPRRLVCI
                     CAPLGLHGPNLFPQQAGRDYESTPYLDLLKDFRDDLTVISGLAHPGVDSGHDSIFSFL
                     TAAQHPERRAGFKNSISLDQLAAEKIGTQTRFPSLTLSAEGFSLSWTRSGALVPSDVW
                     PASVFARLFLEGKPEEVQKQIRRLQDGRSILDQVGDQARRMQAGLGAGDRDKLDEYFS
                     SVRELEQRLAANEEWSHKPKPKVEAQQPQNAMNAADLVGKTKLLFDLTHLAFQTDSTR
                     LITIMLLGTSLVPPIPGVSDGHHNLSHHGQDPGKLEQLRKVELEKMRLLAELLGKLKQ
                     SQEAGGSLLDRTSVFFSSNLGNASSHSCTNLPVILAGGGFKHGQHLAVDASTPLSNLY
                     VSLLQRLGLEIGEFGTSTGTLTGLEVAG"
     gene            complement(4030..6414)
                     /locus_tag="VFB80_12350"
     CDS             complement(4030..6414)
                     /locus_tag="VFB80_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN34607.1"
                     /translation="MRTVLASTLLVLPLWSAFAAAPPENVRAYLTGHCTKCHSGEKPK
                     GKLDLESLAADFSVDADRRRWQIVAERVKAGTMPPKTQPRPAAGDVQMLTDWIGSQAA
                     AAETARRAAHGRTVLRRLNRVEYENTVRDLLGIEVDLKEALPADTAADGFDNAGLALH
                     TSSFLLEKYLDAAETALNLAIVNGPQPPLVKKRFSFKDERAVKVATEKVFLARDDDLV
                     MFSSSPWNSITTGQFYPQNRGIYRIRVSASGHQSAGQPVSFQIDAGPMLMGTKNHLVG
                     YFEAAADQPTIVEFTDHFEARNTLRVLPYGLATAQAVNKIGAEKYEGPGLAVQWVEYE
                     GPLYDQWPPASHRRIFGDLQQAKVPIQSDRNHLEVVSSDPASDAKVILRRFARRAFRR
                     TVTDDDIAPFVALFEQKLAAQYTFEQAVRVALKGVLVSPEFLFFRERPGRLDDFALAS
                     RLSYFLWSTMPDDELLSLAGEGKLEQPGVLREQVERMLKSPQSRALTENFLSQWLGLR
                     DIDFTVPDHLMYPEYDDLLKTSMVEETQRFFEEILKEDLSLTNFVASDFTFANERLAR
                     HYGIPGVKGHALVKVALPPELHRGGVMTMGSVLKVTANGTTTSPIVRGAWVLERIMGT
                     PPPPPPGGVPAIEPDIRGATTIREQLAKHRQIESCANCHKLIDPPGFALESFDVIGGW
                     RDFYRTRGGGKAVVVDGKRMYYGRGKDIDPADELQGGRRFQNIDEFKQLLLEDKDQLA
                     RALATKLLTYATGRAPEAADKAEVDAVVAKIRDKNYGLRSLVHEIVQSSIFQSK"
     gene            complement(6411..>7853)
                     /locus_tag="VFB80_12355"
     CDS             complement(6411..>7853)
                     /locus_tag="VFB80_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34608.1"
                     /translation="VGISEDIVLSGDDVFEVLGTAEKPCRLDANGQQIRTSGDWHGRV
                     QIQHCELRAFGSAARPAIDLTAAGSGEQIVIEHTAFLSSGAIHLNNAGESATVFCHNR
                     LDANSYLPVTNQPDSSPPVFRATGRSPAKKRFQGNFVGKSIVLFEDTRNWQIGGLQDE
                     KSNVCIGLRSSLSIFRSAEMHVCGNYVHTEIPSFRWSQVHTLQVGGPSPGLVVEHNIL
                     RHGQWVVRGLEGEFRYNLVLDADGHNFIIGPRAGTHIHHNIFARYTTVDPNLNATIGV
                     IYKGDGIEIFNNTFDGGGPDTARPWHVPTIEVGPEAHLASLRNNVFYNHPTRFSSGSA
                     TVRPGFSEKQTSPGPPRLGYADFNLFYNPDARERQNYALSVAGKTERTDAGFGLHDVP
                     AGGETNAQVKPQFRGPIPRKFEFSDDEIASRKVTVRQILAAYRRAYSPAEGSPLIAAG
                     DPADGAGSFIGAVGAGERSPGDRFGNWEQP"
BASE COUNT         1285 a         2598 c         2569 g         1401 t
ORIGIN      
        1 gaagtcctgg tgatcgatgg gctgcggccg gagcgcggcg acgcgcaact ctggcggcag
       61 gacacgatcg agcccgacca gagctcgcgg caagccacga tctacacgca gcagcggacg
      121 acgaacaacc cgctgctcgg cccgcgactg atcaattacg acgccacgaa ccgcatgaac
      181 ttcaacagcg ggccgctgct caccggcggc ttctgcttcc agcggggccg cacgctgttc
      241 tgcgtcgatc cgctcaccgg ccagtcgctc tgggaacggg ccctaccctg gccgcaggca
      301 gaaatcttcg gcgacgacga actgctcttc gtttccgacg cgaccggcca acaaacactc
      361 atgctcagcg ccatcgacgg ctcgctgctc agccgccgca aactcgagcc ggccgaacgc
      421 cgctgggcga cctgcggccg caacgtgctc gcgtttgaag ccgccggcac cacgattcag
      481 ctccgactct acgacgccag ccggcagccc gatccgacag aaccggctgc ggctgctggc
      541 gaagcgctct ggacgcggac cgttccgctc gggactcgcg gctgtaccat cgacggcgag
      601 gcgatcggac ttttggagcc cggcgggcag ttcacggtcg tctcgctggc cagcggccag
      661 gtgcgactcg cggtgccgct ggagccggag ccgtcgctct cctggatcca ggttcagacg
      721 tcgcgcgatc agctggtgct gctcgccagt caggagcagg gcgacccgac aggcaacgtg
      781 atgatgcagc cgctgcaaac tgtcaataat ccgctgggcc ggatgcacgg ccgcgtgtat
      841 gccttccagc ggaagacggg caaactgcag tggcaaaccg cggcgttcat ctcgcagcac
      901 ggcatgccgc cggaccagcc ggccgagtcg ccgctgctat tcttcgtgcg caatcggacg
      961 gaggccggcc gcggcaatac gcgatcgacg acgtcggtgc tggccctcga tcgccgcgac
     1021 ggccggccag tctacgagaa cgattcggtg ctgccgcaaa tcgctaacaa ctgcgaagtc
     1081 tcggtcgagc cggccaaaca gtcggtcacg ctcagcctct acggcggcgc caacaagacg
     1141 ctggtcttca agctcagcga caagcccact ccgccgcagc cgccagccca gacgggctca
     1201 ttggccagca gcaccgcggg gcaagcgccg ggaatcgtcg atggcacggt cggcgcagct
     1261 gtcgaactgc tgaatcgcgg gctcaacccg cggattctgc tcccccgcgc cgctccgccg
     1321 ccgccgccgg cccggccgtg aaaagtttac cgccgcacct gcaagttgta gttccgcgtc
     1381 gcggcctgct ggccgggctg gctgtcgaga atgtacagca cgttgtcatc ggtcttgggc
     1441 ggtgcggagg cgacgggggc caatagcgcc cggcggattt gccacacgtc gtagctcagc
     1501 gcccccagcg aaagcaccag ccacgcgccc gcttccagcg gctgccagcg cgacagcagc
     1561 agcaagaacg gcattcccac ggcggccagc cagccactcg tgtagagcca gccctgccac
     1621 gagatgggaa cgacaccaaa tccaatcgtt ttcgggcgga accactgggg tttgccaaac
     1681 atctcgcact ctccaaagag gctggtttac cgcgggcgga taaaaggatt tcggcggtcg
     1741 gggaggaatc tttggatttc gactgctttt ttccgtacgc ccgcgccacc gttgcaaatc
     1801 caggcgattt gggctgactt ttccaggtct gcggaaagtt ctcgccgctc cgcctttccc
     1861 ggttcgatga agattggcgg aactgcctga aggtccggat ttggcggcgg cagccactcg
     1921 ccgccggcat ttggacagga ttcgcccaca ccggggaggg ggagtcgatg aaggttgcag
     1981 tgtctttggt gaccgtttgc ctgctaagtt tgcccgcgac cgtcctggct caatatgccg
     2041 agccggccga gcacagcatc atccgccaga ccggcttcgg ctggcgcaac cacgtcgagg
     2101 tagctgacgg ctgcggctgc tcggtgccgg tccgggccga ctgctacgac cagccctgct
     2161 gcttccgctg cggcctgcat ccgtgctgct tcctgcagcg cgtccatcgg atgctcgact
     2221 gcctcttgcc gtgcaagaag tgctgctgcc tgttgggcgg gcgctgcggc ggatgctgcg
     2281 gttcgtcgta cgatgacggc gtcggcgggc tgcccggcct gtcggacccg ttcgtcgatg
     2341 acgcgctcgt gccgcccaag ccgatcgccg atcccggcac ggaagtccgc ttccagccgg
     2401 cggctccgcg ccgcctgccg tcgcttcctc cgcggaccat cgtcaacaag cccgcgcccg
     2461 taagccggcc cgcggcgacg actcagcccc ggccctcgcc ttacaaggtg accgctccgc
     2521 cggcggcgca gccggtcgaa tatcgcgtca gccacgcgcg acaggcggcc gcgagtcccg
     2581 cgcagccgca cctggtccgc ccggcccagc aatcgatcct gcgccgcatc tcggccgacg
     2641 agcccgcgcc attcgaggtc gaagccagct cgcttcagcc gatcgtccgt tcgcagtcgc
     2701 cgccggccga ggctgcggac gacgccatcc cgcacaatcc cctgcggagc cggtaggcaa
     2761 tctcagcccg cgacctccag cccggtcagc gtgccggtgc tggtgccgaa ctcgccgatc
     2821 tccaggccca gccgctggag caggctgaca tacaggttcg agagcggcgt gctcgcgtcg
     2881 acggccagat gctggccgtg cttgaatccg ccgccggcga gaatcaccgg caggttcgtg
     2941 caggagtggc tgctggcgtt gcccaggttg ctgctgaaaa acacgctggt gcggtcaagc
     3001 agactgccgc ccgcttcctg cgactgcttg agcttgccca gcagctcggc cagcagcctc
     3061 atcttttcca gctcgacttt gcggagctgc tcgagcttgc ccggatcctg cccgtggtgc
     3121 gagaggttgt ggtgcccgtc ggagacgcca ggaatcggcg gcaccaggct cgttcctagc
     3181 agcatgatcg tgatcagccg cgtcgaatcg gtctggaagg ccaggtgcgt caggtcgaac
     3241 agcagcttcg tcttgcccac aaggtcggcc gcgttcattg cgttctgcgg ctgctgggct
     3301 tcgaccttcg gcttgggttt gtgcgaccac tcttcgttgg cggccagtcg ctgctcgagc
     3361 tcgcggacgc tactgaagta ttcgtcgagc ttgtcgcggt cccccgcgcc gagaccggcc
     3421 tgcatccgcc gggcctgatc gccgacctgg tcgaggatgc tccgcccgtc ctggaggcgg
     3481 cgaatctgct tctgcacttc ctcgggcttg ccttccagga acaagcgtgc gaagaccgac
     3541 gccggccaga cgtctgacgg cacgagcgcc ccactgcgcg tccaggacag gctgaagccc
     3601 tcggccgaga gcgtgaggct gggaaagcgg gtctgcgtac cgatcttctc tgccgcgagc
     3661 tggtcgagcg aaatgctgtt cttgaatccg gctcgccgct ccgggtgctg ggccgccgtg
     3721 aggaaactga agatcgaatc gtgcccggaa tcgacgcccg gatgcgcgag gcccgagatc
     3781 accgtcaggt cgtcgcgaaa atccttgagc aagtccagat agggcgtcga ttcatagtcg
     3841 cggccagcct gctggggaaa caggttcggg ccatgcaatc ccagcggtgc gcagatgcag
     3901 accagccggc gcggcggatc ggcattcaga gcgccgcgcg attggcgatc gagcacggca
     3961 tcgaagagcg gcaatgccag cgagactccg cctgcgcgaa ggaaactgcg tctggaaagc
     4021 ttcattggat tacttgctct gaaaaatgga actttgcacg atctcgtgaa ccagcgatcg
     4081 caaaccatag tttttgtcgc ggattttggc gaccaccgca tccacttcgg ccttgtcggc
     4141 cgcctccggc gcacggccgg ttgcataagt gagcagcttc gtggccagcg cgcgggcgag
     4201 ctggtcttta tcttccagca ggagttgctt gaactcgtcg atgttctgga atctgcggcc
     4261 gccctgcagc tcgtcggcgg ggtcgatgtc cttgccgcgg ccgtagtaca ttcgcttgcc
     4321 gtccacgacg acagccttgc cgccgccgcg ggtacggtag aagtcgcgcc agccgccgat
     4381 gacgtcaaaa ctctccaggg cgaagccggg cgggtcgatc agcttgtggc aattcgcgca
     4441 cgactcgatc tgccggtgct tggcgagctg ctcgcggatc gtcgttgctc cgcggatgtc
     4501 cggctcgatc gccggcacgc cgccgggcgg aggcggcgga ggtgttccca taatccgctc
     4561 cagcacccac gcgccgcgga cgatcggcga agttgtcgtg ccgttggcgg tgaccttgag
     4621 gacgctgccc atcgtcatca cgccgccgcg gtgcaattcg ggcggcagcg cgaccttgac
     4681 cagcgcgtgg ccttttacgc cgggtatgcc gtaatgccgg gccagccgct cgttggcgaa
     4741 cgtgaagtcg ctggcgacga aattcgtgag gctgaggtcc tccttgagaa tttcctcgaa
     4801 gaaccgctgc gtctcctcga ccatcgaggt cttcagcagg tcgtcgtatt ctggatacat
     4861 caggtggtcg ggcacggtaa agtcgatgtc ccgcaggccc agccactggc tgagaaagtt
     4921 ctcggtcagc gcccgcgact gggggctttt cagcatccgc tcgacctgct cgcgaagcac
     4981 gccaggttgc tctagcttgc cttcgcccgc aaggctcagc agctcgtcgt ccggcatcgt
     5041 gctccagagg aaatacgaca atcggctggc cagggcgaag tcgtcgagcc ggcccggccg
     5101 ctcgcggaag aacagaaact ccggcgacac gagcacgcct ttgagggcca cccgcacggc
     5161 ctgctcgaac gtgtactgcg cggccagctt ctgttcaaac aacgcgacaa agggcgcgat
     5221 gtcgtcgtcc gtgaccgtgc ggcgaaacgc ccggcgggcg aagcggcgga ggatcacttt
     5281 cgcgtcgctg gccggatcgg acgagacgac ttccaggtgg ttgcggtcgc tctggatcgg
     5341 gaccttggct tgctgcaggt cgccgaagat gcggcggtgg ctcgccggcg gccactgatc
     5401 gtagagcgga ccctcatatt cgacccactg caccgccagg ccgggccctt cgtacttctc
     5461 cgcgccgatc ttgttcaccg cctgggccgt ggccaggccg tagggcagga cccgcagcgt
     5521 gttgcgggcc tcgaagtggt cggtgaattc gacgatcgtc ggctggtcgg cggcggcctc
     5581 gaagtagccg accaggtggt tcttcgtccc catcagcatc gggccggcgt cgatctgaaa
     5641 gctcaccggc tggcccgcgc tctgatgccc gctggctgac acgcggatcc ggtagatgcc
     5701 gcggttctgc ggatagaact gccccgtggt gattgagttc cacggagagc tgctgaacat
     5761 caccagatcg tcgtcgcggg ccagaaacac tttctcggtc gcgaccttca ctgcccgttc
     5821 gtccttgaag ctgaaccgct tcttcaccag cggcggctgc ggaccgttca cgatggccag
     5881 gttgagcgcc gtctcggcag cgtcgaggta tttctcgagc aggaaagacg aagtgtgcag
     5941 ggccagcccg gcgttatcga agccgtcggc cgcggtgtcc gcgggcaagg cctccttcag
     6001 gtccacctca atacccaaga ggtcgcggac cgtattctcg tattcgacgc ggttcagacg
     6061 gcggagcacc gtgcggccgt gggcggcccg ccgcgcggtt tctgcggccg cggcctggct
     6121 gccaatccag tcggtcagca tctgcacgtc gccggccgct ggccgcggct gagtcttggg
     6181 cggcatggtt cccgccttca cgcgctcggc cacgatttgc cagcgccgcc ggtcggcatc
     6241 aacactgaaa tcagccgcga gggactccag atcgagcttc cccttgggct tttcgccgct
     6301 gtggcacttg gtgcaatggc cggtcagata cgcgcgaacg ttctccggcg gcgcggccgc
     6361 gaaagcgctc cagagcggca ggaccagcag cgtggacgcc aacaccgttc tcatggttgc
     6421 tcccagttgc caaaccgatc gccgggcgat ctttctccag cgcccacggc tccgataaac
     6481 gagcctgcac cgtcggccgg gtcgccggca gcgatcagcg ggctgccttc ggcagggctg
     6541 tacgcccgcc ggtacgcggc caggatttgc cggaccgtga ccttgcggga agcaatctca
     6601 tcgtcgctga attcgaactt gcggggaatc ggcccgcgaa attgcggctt gacttgcgcg
     6661 ttcgtctcgc cccctgcggg cacgtcgtgg aggccgaagc cggcgtcggt gcgttcggtt
     6721 ttgccagcaa cggagagggc ataattctgt cgctcgcggg cgtcggggtt gtaaaagagg
     6781 ttgaagtccg cataacccag ccgcggaggg ccgggcgagg tctgcttctc gctgaagccg
     6841 ggccgcacgg tcgcgctgcc gctgctgaac cgtgtgggat gattgtagaa gacgttgttc
     6901 cgcaggctgg ccaggtgcgc ctcggggccc acctcaattg tcggcacgtg ccagggacgg
     6961 gccgtgtcgg ggccgccgcc gtcgaacgtg ttgttaaaga tttcgattcc atcgcccttg
     7021 tagatcacgc cgatcgtcgc gttgaggttc ggatcgacgg tcgtgtagcg ggcgaaaatg
     7081 ttgtggtgga tgtgcgtgcc cgcccgcggg ccgatgatga agttgtgccc gtcggcgtcg
     7141 agcacgaggt tgtagcggaa ctcgccttcg aggccgcgga ccacccattg cccgtgccgg
     7201 agaatgttgt gctcgacgac cagcccgggc gacgggccgc cgacttgcag cgtgtggacc
     7261 tggctccagc gaaagctggg aatctcggtg tggacatagt tgccgcagac gtgcatctcg
     7321 gccgagcgaa aaatgctcag cgacgaccgc agcccgatgc atacattgct tttctcgtcc
     7381 tggaggccgc cgatctgcca gttcctcgtg tcttcgaaga gcacgatcga tttgccgacg
     7441 aagtttccct gaaatcgctt tttggccggc gagcggccgg tggcccgaaa gaccggcggc
     7501 gaagagtccg gctggtttgt caccggcagg taggagttcg cgtccagccg attgtggcag
     7561 aagactgtgg ccgactcgcc agcgttattc aggtggatcg cgccggacga cagaaaagcc
     7621 gtgtgctcga tgacaatctg ctcgccgctg cccgccgcgg tcaggtcgat cgccggccgc
     7681 gcggcgctgc caaaagcccg cagctcgcaa tgctgaatct gtacgcgccc gtgccagtcg
     7741 ccgctggtgc gaatttgctg gccattggca tccaggcggc agggcttctc ggcagtgccc
     7801 agcacttcga agacgtcgtc gccgctcagg acaatgtcct cggaaatgcc gac
//
LOCUS       DASRJB010000470         9258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1540640, whole genome shotgun sequence.
ACCESSION   DASRJB010000470 DASRJB010000000
VERSION     DASRJB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540640"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..2130)
                     /locus_tag="VFB80_12360"
     CDS             complement(223..2130)
                     /locus_tag="VFB80_12360"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZN34609.1"
                     /translation="MSYALRRTLEELAAAGHLVRLEDEIDPRLEAAEIQRRVYARGGP
                     AVLFTKVQGCRFPLVSNLFGTIERARYLFRHTYDSVRQAIELKIDPSRALANPLKYAS
                     APLTAWRMQPASVRTGPVLANETTIGQLPQVVSWPQDGGAFITLPQCYTEDVNQPGTA
                     HSNLGMYRVQLSGNDYVPDREVGLHYQIHRGIGVHHRAALQAGQPLRVSIFVGGHPAM
                     TLAAVMPLPEGMSELTFAGALAGQRIPLIQRAGQLPIYADADFCIQGQVDPAAPQKLE
                     GPFGDHLGYYSLAHEFPVLKVERVYHRQDAVWPFTVVGRPPQEDTTFGQLIHELTGDV
                     IPTVLHGVKAVHAVDAAGVHPLLLAVGSERYMPFLPNPRPQELLTQASAILGQGQLSL
                     AKFLLIVNRYDDESLDIHDIPAFLQHLLRRVDWRRDLHFHTNTTIDTLDYSGEGLNMG
                     SKVVIAAAGPPVRELPSELPGDLRLPEGFREPRVVLPGVLVVEGPRVQDGGTERRRDG
                     ETERSSHSLPFSPSLRPSVSPSESPEIARLCEAVDTAFPLLVVVDDADFAARNLANFL
                     WVTFTRANPAADVYGVGAFTERKHWGCTGPLVIDARMKPHHAPPLAPDPEVAKRIDAL
                     AARGGPLARWL"
     gene            complement(2381..3727)
                     /locus_tag="VFB80_12365"
     CDS             complement(2381..3727)
                     /locus_tag="VFB80_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HZN34610.1"
                     /translation="MFALFGLAIFSTAAAQEADWKVGLARVKVTPEQPVFMAGYASRN
                     KPYESVRDDLYAKAMVIEDAAGTRAVLVTSDLIGFTAEVGDPIRERIAKETGIPAASV
                     LLNASHTHTGPELSLDPKPEGNRALADVERTIAYTKQVQDRVVQVVKEAAARLAPAKL
                     AWGVGVVHFPMNRREFTIERGVILGVNPRGLADRSVPILRIDDPAGQLRAVVFGAACH
                     NTTLGGQHYDISGDFTGRAQRQIEAPHSGIETLFVQGCAGDANPYPRGSYELADQHGD
                     ELAREVERLLTSKLVPIRGPLKVAAGEVTLPLAIATTKDELEKDAAAKTGYKATAARQ
                     ALTRLERGEKVATEYSCPLAVWQFGGDLTLVALSGEVVVEYVKLIEEALGPNRLWVAA
                     YTNDVYGYLPSARTLTEGGYETRGLYSGGIGQFAPAAQNALVEKATDLARSAGRSK"
     gene            complement(3758..4810)
                     /locus_tag="VFB80_12370"
     CDS             complement(3758..4810)
                     /locus_tag="VFB80_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZN34611.1"
                     /translation="MRPACCLPLVVAALAGLGVRAGAEERRFARSELHMGVEFEVVLY
                     AEDEARASAALTKAMARIAALDKALSDYDLESELSRLSATSHVTSPTPPAEFPAVSVS
                     DELLTVLATGQEISRASGGAFDVTVGPLTRLWRRARRQRELPDAELLKTAQASVGYKF
                     LQLDAASKTARLQRPNMRLDLGGIAKGFAADEAVKAVVGCGLPRVLVRGSGDIACWDP
                     PPGQAGWTIGVAPLDPDDPPQEFISLRRLAVSTSGDARQHLVVDGKRYSHILDPRTGQ
                     PVSGRSSVTVIATSGIIADALDTAASVLGPEQSLALLKQYPGSELLMTVEDAAGLRHR
                     TQSPEFQKYETTKPTP"
     gene            4874..5902
                     /locus_tag="VFB80_12375"
     CDS             4874..5902
                     /locus_tag="VFB80_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34612.1"
                     /translation="MDCRRYALMGLLFLLAATLEAQTSLRPEEGVVLLRNGFILSGLV
                     TQAGDFYIVTLGETGEVRLPSADVEALCGSLDEVYEFKVRHLSGSGVKPHLALAEWCL
                     RQNLPRHCSQQLILAMRIDPDNEQLKHLERRLELAIATPPPPAAAPQPAIAAPSNDQL
                     EKSLRTLPPGSIEKFSAVVQPILLNRCGANQCHGPAAKSEFRLLRPPEGQIASRRFTQ
                     RNLHAALAQLDMQNPEASPLVKLPQGQHGSSLAPIFDKQTQHQLAELLAWVKLTAPPP
                     PAPAVPATIGPANQTLSQPAGSAVAAGPPLETAPPAGPPQPAAGPATFTPRDRYDPEI
                     FNRRYHGK"
     gene            complement(5877..6035)
                     /locus_tag="VFB80_12380"
     CDS             complement(5877..6035)
                     /locus_tag="VFB80_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34613.1"
                     /translation="MPVANPLEVESFAWLTAAEIRQLPDLLASNLEFLAALEGNDIAG
                     LTSRDSGG"
     gene            complement(6152..6316)
                     /locus_tag="VFB80_12385"
                     /pseudo
     CDS             complement(6152..6316)
                     /locus_tag="VFB80_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(6576..7277)
                     /locus_tag="VFB80_12390"
     CDS             complement(6576..7277)
                     /locus_tag="VFB80_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34614.1"
                     /translation="MSGLSLALVALAATACLAETPDLSGARTPPARLLSGGRMFNGQN
                     WWTRFGEPVNAAALSASDGARGDVAPVAMYGHDYIYGPGACDCPPPCIDDLWGGYYQH
                     PKRCDGYTGLFHRGHCGHCGRAGCDGGCGKGLGCGKGACGCGVALGCSKPVDSCCAPI
                     CGGGHLGHKWKHMKAHWCSRDCDSCAKPLGCGCSTPLAPFAPEVQVPSEKQALVRPEP
                     LPEDAALIQLPRLNN"
     gene            7640..8557
                     /locus_tag="VFB80_12395"
     CDS             7640..8557
                     /locus_tag="VFB80_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34615.1"
                     /translation="MPTRPTEAIVAAVTLATGAAILALHQRRIRQTTLTSAWCWSWAA
                     LAGWAGVELLAAWRSDLTALALAPLRLAAVALSFCPAVSLVGAKRPQHAAWNIVVVSL
                     WGIVALPAAENYFLHPGQRLALGDARGWFLWILILLGPINFLPTRFWLAALLVAVGQV
                     VALSRYVALPQRPPVPQPELVGLALCGLGMLSAAASARRKTIAANPLDRLWLDFRNSF
                     GLFWSLRVQERMNAASAQHGWDVELGWRGFRRREEALKPEAQAKEPSDSPSLAIQSSE
                     PALRTTFKGLLRRFVSGEWIAERMGQSLD"
     gene            8562..>9258
                     /locus_tag="VFB80_12400"
     CDS             8562..>9258
                     /locus_tag="VFB80_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN34616.1"
                     /translation="MTAPSPAALPARYCHSLDGLNRLLEVTRTLAEEVDLAPMLDAIV
                     REACHALRCDRALLYQFDAKRNVLSATAGIAEGLIVPLDCGIAGHVARCGAMLNVPDA
                     ARDSRWDPAYDNLVGYQTKTVLAAPLIAARDGRLLGVLEMLNNAGGPFDSDDEALAVA
                     FSCHAAAALDRARLIDEIHKRRELEHSLLAAREVQRRFMPGSLPAIAGYEVATWWFPN
                     EAVGGDYCDVVPLA"
BASE COUNT         1474 a         3139 c         3088 g         1557 t
ORIGIN      
        1 caccgccgtt gccgccggga tcgccacgac ggcctggcac ttggggcact tgccgcgctt
       61 tcccgcgaat tcgtcgcgca cgctgaccgt gtgctgacat tggccgcagg tgacggaaat
      121 cggcatggtc gagactcgtg cgagcccgcg ctgttttcgc aggaattatc ggttcgcagg
      181 caccggcgcc gcaagcgcgg cccgccggct gcgacgattt gttcacaacc acctggccag
      241 cgggccgccg cgggcggcga gggcgtcgat ccgtttggcg acttcgggat ccggcgccag
      301 cggcggcgca tggtgcggct tcattcgggc gtcgatcacc agcggcccgg tacagcccca
      361 atgcttccgc tcggtgaacg cccccacgcc gtaaacatcc gccgccggat tggcccgcgt
      421 gaacgtcacc cagaggaagt tggccaggtt ccgcgccgcg aaatccgcgt catcgaccac
      481 gacgaggagc ggaaaggcgg tgtcgaccgc ctcgcagagt cgggcgattt ctggcgattc
      541 ggaaggagag acagagggac ggagagaagg agagaagggg aggctgtggg atgatcgctc
      601 tgtctctccg tccctccgtc tctccgtccc tccgtcctgc acccgcggtc cctccaccac
      661 caacaccccc ggcagcacca cccgcggctc tcgaaaacct tctggcagcc gcaagtcgcc
      721 gggcagctcg ctcggcagct cgcgcaccgg cggaccggcg gcggcgatga ccaccttgga
      781 gcccatgttc agcccctcgc ccgaatagtc gagcgtgtcg atcgtggtgt tggtgtgaaa
      841 gtgcaggtcg cggcgccaat cgacacgccg cagcaggtgc tgtaagaacg cggggatgtc
      901 gtgaatgtcc agcgactcgt cgtcgtagcg gttgacgatc agcaggaact tggcgagcga
      961 gagctgcccc tgaccgagaa tcgcagaggc ctgcgtcagc aattcctgtg gccgcggatt
     1021 gggtaggaat ggcatgtacc gctcgctgcc gacggcgagc agcaacggat gcacaccggc
     1081 cgcatcgacc gcgtgcacgg ctttcacgcc gtgcaggacg gtcggaatca cgtcgccggt
     1141 cagctcatgg atcagttggc cgaaggtcgt gtcctcctgc ggcggccggc cgacgacggt
     1201 gaacggccag accgcgtcct ggcggtgata gacccgctcg accttcagca cgggaaactc
     1261 gtgggccaga ctgtagtagc ccaggtgatc gccgaacggg ccttcgagct tctgcggcgc
     1321 ggccggatcg acctggccct gaatgcagaa atcggcgtcg gcatagatcg gcagctgacc
     1381 ggcccgctgg atcagcggaa tccgctgccc ggccaaggcc ccggcgaacg tcagctcgct
     1441 catcccttcc ggcagcggca tcaccgccgc cagcgtcatc gccggatgtc cgccgacgaa
     1501 aatgctcacc cgcagcggct ggccggcttg caaggccgcg cggtggtgca cgccgatccc
     1561 gcggtgaatc tggtagtgca ggccgacctc gcggtccggc acatagtcgt tgcccgaaag
     1621 ctgcacccgg tacatgccca ggttgctgtg cgccgttccc ggctgattca cgtcctcggt
     1681 gtagcattgc ggaagcgtga tgaacgcccc gccgtcctgt ggccagctca cgacctgcgg
     1741 cagctggccg attgtcgtct cgttggccag caccggcccc gtccgcacgc tcgccggctg
     1801 catccgccag gcggtcagcg gggcggaggc atacttcagc gggttggcca gcgcccgcga
     1861 gggatcgatc ttcagctcga tcgcctgccg cacgctgtcg tacgtgtggc ggaacaaata
     1921 ccgcgcccgc tcgatcgtcc cgaacaggtt cgagaccagt ggaaaccggc agccctgcac
     1981 tttggtgaac aggacggccg gcccgccacg ggcatagacc cgccgctgaa tctccgccgc
     2041 ctccagccgc ggatcgatct cgtcttccag gcgcaccaga tgcccggcag cggccagttc
     2101 ctcgagcgtg cggcgaaggg catacgacat gcgcgcatac gaaacaaaaa agtccggact
     2161 aggatttctg ccgcaactcc attggtttca atgacttgcg ttcaattctg cggcacagtg
     2221 attatgcgca ttatgcgcgc ttggctccaa ctaccagtcg aggttgtcga aacggctgaa
     2281 gcgaaattgg aagtgcatat atagctcttt gtaagccaga cactacgtat tatacagttg
     2341 tatgaaatcg agggcaatgc agtaaccgat gggccgcggc ctatttgctc cgtcccgcgc
     2401 tacgggccaa atcggtggct ttctcgacga gggcgttctg ggcggcgggg gcgaattggc
     2461 caatgccgcc ggagtagagg ccgcgggtct cgtagccgcc ttcagtcagc gtgcgggcgg
     2521 aagggaggta gccgtagacg tcgttggtgt aggcagcgac ccagaggcgg ttggggccga
     2581 gggcctcctc gatcagcttt acgtattcga cgacaacttc gcccgagagg gccaccagcg
     2641 tgaggtcgcc gccgaactgc cagacggcca gcgggcacga atactcggtg gcgactttct
     2701 cgccccgctc gaggcgcgtc agggcctgcc tggctgccgt ggccttgtag ccggtcttgg
     2761 ccgcggcgtc cttttcgagt tcgtccttgg tggtagcgat cgccagcggc agcgtcactt
     2821 cgcccgcggc gaccttcagt gggccgcgaa tgggaaccag cttgctcgtg agcagccgct
     2881 cgacttcgcg ggccagctcg tcgccgtgct ggtcggccag ctcatacgag ccgcgcggat
     2941 acggattggc gtcgccggcg cagccctgga caaagagcgt ctcgatgccg ctgtgggggg
     3001 cttcaatctg ccgttgggct cggcccgtga agtcgccgct gatgtcgtag tgctggccgc
     3061 cgagcgtcgt attgtggcag gccgccccga acacgacggc ccgcagctgg cccgccgggt
     3121 cgtcgatccg caggatcggc acgctgcggt ccgccagccc gcgcgggttg acgcccagga
     3181 ttacgccgcg ctcgatcgtg aactcgcggc ggttcatcgg aaagtgaacg acgccgacgc
     3241 cccaggcgag cttcgccggt gcaagccgcg cagcggcctc cttcacaacc tgcacgacgc
     3301 ggtcctgcac ctgtttcgtg taggcgatcg tccgctcgac gtcggccagc gcccggttcc
     3361 cctcgggttt tggatcgagg ctcagctccg gcccggtgtg cgtgtgcgag gcattgagca
     3421 gcaccgacgc ggccggaatg cccgtttctt ttgcgattcg ctcgcggatc ggatcgccga
     3481 cctccgccgt gaagccgatc aagtccgagg tgaccagcac ggcccgcgtg cctgctgcgt
     3541 cttcgatgac catggccttg gcgtacaagt cgtcgcggac gctctcgtag ggcttgttcc
     3601 gcgaggcata gccggccatg aacaccggct gctcgggcgt gaccttgacc cgcgcgaggc
     3661 cgaccttcca gtccgcttcc tgggcggcgg ccgtggagaa aatcgccaga ccgaacagag
     3721 cgaacatagt gttccgcatc ggtgttgctc cggaggccta cggtgttggt ttcgtggttt
     3781 cgtatttttg gaattcgggc gactgggtcc tgtgccgcag gcccgcggcg tcttcgaccg
     3841 tcatcagcag ctcactgccg ggatattgct tgagcaatgc cagcgactgc tcgggaccga
     3901 ggacgctggc cgcggtatcc agcgcgtccg cgatgatacc gctcgtggcg atgaccgtca
     3961 cgctgctgcg gccggagaca ggctgaccgg tgcgcgggtc gagaatgtgc gaataccgtt
     4021 tgccatcgac gaccaggtgt tgccgcgcgt cgccggaggt cgaaacggcc aggcggcgca
     4081 gcgaaatgaa ctcctgaggc ggatcatccg gatcgagcgg cgcgacgccg attgtccagc
     4141 cggcctgtcc gggcggagga tcccagcagg caatatcgcc gctcccccgc accaggacgc
     4201 gcggcaggcc gcagccgacg accgccttca cggcttcgtc ggcggcaaag cccttggcga
     4261 tgccgcccag atcgagccgc atgttgggcc gctgcaggcg ggcggtcttc gaggccgcgt
     4321 cgagctgcag gaacttgtag ccgacgctgg cctgcgcggt tttcagcagc tcggcatcgg
     4381 gcagctcgcg ctggcggcgg gccctccgcc agagcctggt cagcggcccg accgtgacgt
     4441 cgaacgcgcc gccgctggcc cggctgattt cctgccccgt ggcaagcacg gtcaacagtt
     4501 catcgctgac actgaccgcc ggaaactccg ccggcggcgt cggactggtg acgtgcgacg
     4561 tcgcgctgag gcggctcagc tcgctttcca ggtcgtagtc ggagagcgcc ttgtcgagcg
     4621 ccgcgatgcg ggccatcgcc ttggtgagcg cagcgctagc ccgcgcttcg tcctcggcgt
     4681 agagcacgac ttcgaattcg acgcccatgt gcagctcgct gcgggcgaag cggcgctctt
     4741 ccgcgccggc acgcacccca agtccggcca gggcggcgac aacgagcggc aagcagcaag
     4801 caggtcgcat ggcagcatga tagcacggtt tgacattgac cctggggtaa acggccagta
     4861 ccgttccgcg cctatggatt gccggcggta tgcgctgatg ggcctgttgt tcctgctcgc
     4921 ggccacactc gaggctcaaa cctcgctgcg gccggaggag ggcgtggtcc tgctgcgcaa
     4981 cggctttatc ctctccgggc tggtcacgca ggccggcgac ttctacatcg tgacgctcgg
     5041 cgaaaccggc gaggtccgcc tgccctcggc cgacgtcgaa gcactgtgcg gctcgctcga
     5101 cgaggtttac gaattcaagg tccgtcattt atcgggcagc ggcgtcaagc cgcacctggc
     5161 gctggccgag tggtgcctgc gacagaacct gcctcgccat tgctcgcagc agctaatcct
     5221 ggcgatgcgg attgatcccg ataacgagca actcaaacac ctggagcggc ggctggagtt
     5281 ggcgatcgcc actccaccgc cgccggccgc cgcgccgcag ccggcgatcg ccgcgcccag
     5341 caacgatcag ctggaaaaat cgctccgcac gctgccgccg gggagcatcg aaaagttctc
     5401 ggccgtcgtg cagccgatcc tgctcaaccg ctgcggggcc aatcagtgtc acggcccggc
     5461 agccaagagc gagttccgcc tgctgcgtcc gccggagggg caaatcgcca gccggcgttt
     5521 cacgcagcga aacctgcatg ccgcgctggc gcagctcgac atgcagaatc cggaagccag
     5581 tccgcttgtg aagctgccgc agggccagca cggctcgtcg ctcgccccga tcttcgataa
     5641 gcagacgcaa caccagctgg cggagctttt ggcctgggtg aagctgaccg cgccgcctcc
     5701 acccgcgcct gcggtcccgg cgacgatcgg tccggcgaac cagacgctat cgcagcccgc
     5761 cggcagcgcc gttgcggcgg gcccgccgct cgaaaccgct ccgcccgccg gtccgccgca
     5821 gcctgccgcg ggaccggcga ccttcacgcc gcgcgatcgc tacgacccgg agatcttcaa
     5881 ccgccgctat cacgggaagt aagacccgcg atatcgttgc cctcgagtgc ggccaggaac
     5941 tccaggttgc tggccagcaa gtccggcagc tggcgaatct cggccgcggt gagccaggcg
     6001 aacgactcca cttccagcgg attggcgacc ggcacgtccg cgtggtcgat ctcggtgagc
     6061 caccaggcag cgccacgccc cagggggtcg tgctacgcca caggagccgc agcggcgtgg
     6121 cgtccaccgc gagctcttcc ttgagttcgc ggcggaccgc ggccggctcg tcctcgcccg
     6181 gctcgatcgc cccaccagga aagcagtggg ccagcggcgc tcgcacgtgc tgcgagcggc
     6241 ggatcaccag gaaccgctcg ccgcgcagga tgacggccac aatgccgcgg cgggaatcgg
     6301 tgggaggagt cgtcattgcc gccagcgcac gaaaaaggtc gtggtacgtc gctccgtcgg
     6361 cgtgccacga cctctatcct gaaacggaat cgctcgagac ggttccgtcc tcccggtgct
     6421 tgggcatcaa tcgtatcggc agtagcgcgg ctagcgcatg agccgttttt tcccgccgtt
     6481 ggggcggtac agtcccaacc gacacagcgc ataaaaacag gcagcgccaa gggcgctgcc
     6541 tgtgaagctg gttggatcgt gccgacaatt ggcggctagt tgttcaaccg cggcagctga
     6601 atcagggctg catcctccgg cagcggctcc ggtcggacga gcgcctgctt ctcgctgggc
     6661 acttgcactt caggagcaaa cggggccagc ggcgtcgagc agccgcagcc cagcggcttc
     6721 gcgcacgaat cgcagtcgcg gctgcaccag tgggccttca tgtgcttcca cttgtggccc
     6781 aggtgaccgc cgccgcaaat cggagcgcag cagctgtcga ccggcttgga gcagcccaga
     6841 gccacgccgc atccgcaagc gcccttgcca cagccgagcc ccttgccgca gccgccatcg
     6901 cagccggcgc ggccgcaatg gccgcaatgt ccgcgatgga acagccccgt gtagccgtca
     6961 caccgcttgg ggtgctgata gtagccgccc cacaggtcgt cgatgcaggg gggcgggcaa
     7021 tcgcacgctc ccggcccgta gatgtagtcg tggccgtaca tggccacggg tgcgacgtcg
     7081 cccctggcgc cgtcactcgc cgacaaggcc gcggcattga cgggttcgcc gaagcgggtc
     7141 caccagttct ggccattgaa catgcggccg cccgaaagca gtcgggcggg cggagtgcgg
     7201 gcgccggaca ggtccggcgt ttccgcgagg caggcggtcg cggccagggc gaccagcgcc
     7261 aaagacagtc ctgacatcct catgtaatcg ttcctcctcg aatcgagctt gcttccgggc
     7321 actgccgttt gccgccatct tcgtctcgcg cgcccaccct cggcaccggc gggcgcgcaa
     7381 gcgcaaacgg acccattcaa acatgcccgg caaggcgcgg agcggcaaat gcgagcgttg
     7441 atttttggca acattcgccg tcgccgcggc cgcttgcacc gggggcgggc tgtgtagcgc
     7501 cttgagcgct tggccctccc agctaagatc gaccgttggc cgcgattgtt gcgttcttac
     7561 caccatcgtc ccgactcgcg cggtttttgc agtcattcgg cctgtgctgg cagcgcccgg
     7621 cactcgcgga gtaaggcttg tgccgacgag accgaccgag gcgatcgtag cggctgtgac
     7681 gctggcgaca ggcgccgcga ttctcgcact gcatcagcgc agaatccggc agacgacgct
     7741 gacgagcgcg tggtgctggt cgtgggcggc gctcgctggc tgggcgggcg tggaattgct
     7801 cgcggcctgg cgctcggatc tcaccgcgct ggccctcgcg ccgctgcggc tggcggcggt
     7861 cgcgctcagc ttttgcccgg cagtcagcct ggtgggggcc aagcgtccac agcacgcggc
     7921 gtggaacatc gtcgtcgttt cgctgtgggg catcgtcgcg ctgccggcgg ccgagaacta
     7981 cttcctccat cccggccagc ggctggcgct gggcgatgcc cgcggctggt tcctctggat
     8041 tctgattctg ctcggcccca tcaactttct gccgacccgg ttctggctgg cggcgctgct
     8101 cgtcgccgtg gggcaggtgg tggcgctgtc gcgctatgtc gcgctgccac agcggccacc
     8161 tgttccgcaa ccggagctag tcggcctggc gctctgcgga ctaggcatgc tcagcgcagc
     8221 cgctagcgcc cgccgcaaga cgatcgcagc caatccgctc gaccggctgt ggctcgactt
     8281 tcgcaacagc tttggcctct tctggagcct ccgcgtccag gaacggatga acgcagcttc
     8341 cgcgcagcac ggctgggacg tcgaactggg ctggcgcggc tttcgccgca gagaggaggc
     8401 attaaagcct gaagcgcaag cgaaggagcc gagcgactcg ccttcgctcg cgattcagtc
     8461 gtcagagccg gcgctgcgga ctacctttaa gggcctgttg cggcggtttg tctcgggcga
     8521 gtggattgca gagcgaatgg gccagtccct agactgaccg catgactgct ccctccccgg
     8581 cggctctgcc ggctcgctat tgccacagcc tggatgggct caatcgtttg ctggaagtga
     8641 cccgcacgct ggccgaggaa gtcgatctag cgccgatgct cgacgcgatc gttcgcgagg
     8701 cgtgccacgc gctccgctgc gaccgggccc tgctctatca gttcgacgcc aaacgcaacg
     8761 tgctttcggc gaccgcgggg attgccgaag gtttgatcgt gccgctcgat tgtggcatcg
     8821 ccggccacgt cgcccgctgc ggcgcgatgc tcaacgtgcc cgacgccgcc cgcgattcgc
     8881 ggtgggatcc ggcctacgac aacctggtcg gatatcagac caagactgtg ctcgccgcgc
     8941 cactgatcgc cgcccgcgac ggccggctgc tgggcgtgct cgagatgctg aacaacgccg
     9001 gcggcccgtt cgacagcgac gacgaggcgc tggccgtcgc cttcagctgc cacgccgcgg
     9061 cggccctcga ccgggcccgc ttgatcgacg aaatccacaa gcgccgcgag ctggagcact
     9121 cgctcctggc cgcccgcgaa gtccagcggc gattcatgcc tggcagcctg ccggccatcg
     9181 ccggctacga ggtcgccacc tggtggtttc ccaacgaagc ggtaggcggc gactactgcg
     9241 acgtcgtccc gctggccg
//
LOCUS       DASRJB010000471         5687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3231227, whole genome shotgun sequence.
ACCESSION   DASRJB010000471 DASRJB010000000
VERSION     DASRJB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231227"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1768)
                     /locus_tag="VFB80_12405"
     CDS             complement(218..1768)
                     /locus_tag="VFB80_12405"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HZN34617.1"
                     /translation="MTDKTTARLKLHVEEIPEQPALALPQLAPLCRAFEQATGWQLRY
                     EQSPAGIGEAWSMAIAAGQSAAGRLVLAEPSRDDAETPAAELHQARPLALAIGGLLGE
                     VNRLRGELWHREAELAAGVPVAVRPDEGPHLAERLEAVLKGGAEAVGCQAAGLYLLDE
                     GTSELKLRAAFGLPHERLLAPARPLRGSVADLEALVGHAVVLEDTTLLPHWRCPEDFP
                     SAVCVPVSSPTIPLGTLWVFSQQQRDFTPQETNLLEIIAGRLAADLEREMLLAAGAQA
                     KSRSQQLDAAGQWLTDRLPMIAPLVDDYEVAGWTRPADDLGRGFHDWSVLPDGRLALA
                     VGAAEGRLLAGAMSAASLHAAVKAHAGYRHSAAELLARVNDTLWTASPGDQHAGLAYA
                     VLDEGSGRLELALAGGVGAILIGTEQPRVFVADGLPLGTEPEAVVVKADATLAPGEAL
                     LLVSGGVRCALDAAGLRIGENAIASLVSKHLRDSADDLAARLRKLLDGGEPAADLSIV
                     VVKRRLTS"
     gene            2033..3406
                     /locus_tag="VFB80_12410"
     CDS             2033..3406
                     /locus_tag="VFB80_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34618.1"
                     /translation="MLRLTGRGTAHTCDGFTRRDFMQVGALGAIGLSLPQLVAAQEQG
                     AVRPEHDDRSCILIFNLGAPSQMDTFDPKPDAAAEIRGPFAPISTKGGFQITEILPRH
                     AAVADKFSVVRSCYHTAAAVHDTGHQMLQTGRLFTGGLNTPHVGCALAYLRGRKSDLP
                     PHVILPEAMGPTGGNLPHGQDAGFLGKAHDPFVLNTDPSVANFKVPDLLPPKELGDVR
                     LERRRKLRSIVEQHAAEFEASASAKLMDANFEAAWRMMTSPQARAAFDLSQEPDSVRD
                     KYGRNRFGQCCLLARRLIEAGVRFVTVNTFLTVFDEITWDIHGSKPFTSIEGMKNIVA
                     PMYDQAYGTLIEDLADRGLLAKTLVCNLAEFGRTPRINPAGGRDHWPQCFCAYFAGGG
                     IQGGRAVGKSDAIGAYPAERAVEPAEVVATIYKSLGFDLDTTLPGPAGRPFPLVDFGK
                     HEIKELF"
     gene            3440..4594
                     /locus_tag="VFB80_12415"
     CDS             3440..4594
                     /locus_tag="VFB80_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN34619.1"
                     /translation="MNRPSVAVLTPQGRGAVATIGIRGAGAVQLVDQCVALAAGQPLG
                     SCAAGRVLLGRVGFAGSAAEEVVVGLVAPDEVEVHCHGGLAVAEAVAQALVAAGGRRV
                     GWQEWVQDGAPDPIAAAALLALPQARTERTAAILLAQYRGALRSEVVAIDELLGRGEN
                     SAAGNRLTQLAERADVGLHLTQPWRVVVAGRPNAGKSSLVNALLGYQRAIVFEQPGTT
                     RDVLTAATALDGWPIELADTAGLRAPGNPIEAAGVERAAAAITAADLTLLVAETTAAW
                     DAALHAQVAASARRLLIVHNKSDLAPPPADGRPAGIAVSAKTLAGIAGLAAAITAALV
                     PQPPTVDAAVPFTAEQFTALAEAREQLTRSGAGAARSALTRLLGKLQCPP"
     gene            4591..>5687
                     /locus_tag="VFB80_12420"
     CDS             4591..>5687
                     /locus_tag="VFB80_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN34620.1"
                     /translation="MKPFLPLLLASLTATALAADGFPRVPPTPPEKAEATFETLHGFR
                     MELIAAEPLVASPVDLAYDEDGRAYVVEMRDYPYPEEKNAVPTDFPGTVRLLEDADRD
                     GKYDKATIFADQLAWPTSVCCYKGGVFVAAAPDIWYFKDTDGDGKADVRRKVFTGFSR
                     YNVQAIMNSLRWGLDNKIYGSASGNGGNIRHAERPDDPLIQLTRRDFRFDPVTEKIEA
                     ISGGERFGATFDDWGNRFLCNIRNPVQHVVLPLHYLARNPNLVVPSAIHDCAESGDQL
                     RVYRLSPPEAWREFRAQRWALEQANMPRSETIGAGFWTSSSGVCVYRGAAYPEPFRGN
                     VFIGEVAGNLIHRQILERDGVTFKSRRADENT"
BASE COUNT          924 a         1973 c         1931 g          859 t
ORIGIN      
        1 cagacagtaa tagtgatgca ccaagcaatt tgacaggcca gcccgaaacc acgcgtcgtg
       61 gcgatgcttg gatccgtccg tcccaaagaa tcctaagtgc aacgaccgcc atgattccga
      121 tcacgccgag tgagatcatc ctcgatacct caggatcggc ggattatagt agcccttcaa
      181 tcgactgcaa ccgctcggtc cgatgcaggc gctcgaatca cgaagtaagt cgccgcttca
      241 cgacgacgat gctcaagtcc gcagcgggct cgccgccgtc gagcagcttt cgcaggcggg
      301 cggccaggtc gtcggcggag tcgcggaggt gcttgctcac cagcgaagcg atggcgtttt
      361 cgccgatccg cagaccggcc gcgtcgagag cgcagcgaac gccgcctgaa acgagcagca
      421 aggcctcgcc cggggccaac gtggcgtcgg ctttcaccac aaccgcttca ggctcggtgc
      481 cgaggggtag gccgtccgca acgaagacgc gaggctgctc ggtcccaatc aatatcgctc
      541 cgacgccgcc cgccagcgcc agctccaatc ggccgctgcc ctcgtcgagg acggcgtaag
      601 ccaggccggc gtgctgatcg ccaggcgagg cggtccagag cgtgtcattg acgcgcgcca
      661 atagctcggc cgcgctgtga cggtagccgg cgtgagcctt gaccgcggcg tgcagcgacg
      721 ccgcactcat cgcgccagcc agcagccggc cttccgccgc accgacggcc agagccaggc
      781 ggccatcggg caagaccgac caatcgtgga agccgcggcc cagatcgtcc gccgggcgcg
      841 tccagccggc gacttcataa tcgtccacca gcggtgcgat cattggcagg cggtcggtca
      901 gccactgccc agcggcgtcg agctgctggc tgcggctctt ggcctgggct ccggcggcca
      961 gcagcatctc ccgctcgaga tcggcagcca gccggccggc gataatctcc agcaggttcg
     1021 tctcctgggg cgtgaagtcg cgctgctgct gactgaagac ccacagcgtg ccgagtggaa
     1081 tcgtcgggct cgagaccggc acgcagaccg ccgacggaaa atcctcaggg caccgccagt
     1141 gcggcaacag cgtcgtgtct tccagcacca cggcgtggcc gacgagcgcc tccagatcgg
     1201 cgacggaacc ccgcagcggc cgggccggcg cgagcagccg ctcgtgcggc agcccgaagg
     1261 cggcccgcag tttgagttcg ctcgtgcctt cgtcgagcag gtacagcccc gcggcctggc
     1321 agccgacggc ttccgcgccg cccttgagca cggcctccag ccgctcggcc aggtgcggcc
     1381 cttcatccgg ccgcacggcg accggaactc cggccgcgag ctcggcctcg cggtgccaca
     1441 gttcgccgcg gaggcggttg acctcgccca agaggccgcc gatggcgagg gccagcggcc
     1501 gggcctggtg tagttcggcg gccggcgttt ccgcgtcgtc gcgcgagggc tcagccagca
     1561 cgagccgccc ggccgcggat tggcccgcgg cgatcgccat cgaccaggct tcgccgattc
     1621 cggccggcga ctgttcatag cggagctgcc agccggtggc ctgctcgaag gcccggcaga
     1681 gcggggcgag ctgcggcagg gcgagtgcgg gctgctccgg aatctcttcg acgtgcagct
     1741 tcaagcgggc ggtggttttg tcagtcacgt gcctagcaat cgcgcgagcg gtcggggcgt
     1801 gaagccatcc gtgccgagcg tagggcaggc cgcgggcccg ccctaaacct tccgtgaccg
     1861 tcacagattc catcgaccgc ttcggccggc cgacgtgaac cgccggcggc cagagcactc
     1921 taaccggcag catccgcaga accgggcaat tcgccccggc ggctgcaact cctggcggca
     1981 attgatctag cggccgcgga tggctacgtt gaagcgagcg gaggaactca cgatgctgcg
     2041 actcaccggc cgcgggacgg ctcacacgtg cgacggcttc acgcgccgcg actttatgca
     2101 ggtcggggcc ctgggcgcaa tcgggctgtc gctgccgcag ctcgtcgcgg cgcaggagca
     2161 gggcgccgtc aggccggagc acgacgaccg gtcctgcatc ctgatcttca acctcggcgc
     2221 gccgagccag atggacacct tcgatccgaa gcccgacgcg gcggcggaga ttcgcgggcc
     2281 ttttgcgccg atctcgacca agggcggctt tcagatcacc gaaatcctgc cgcggcacgc
     2341 ggcggtcgcc gacaagttct cggtcgtccg ttcgtgttat cacaccgcgg ccgcggtgca
     2401 cgacacgggc caccagatgc tgcaaaccgg ccggctgttc accggcgggc tcaacacgcc
     2461 gcacgtgggc tgcgccctgg cctatctccg cggccgcaag agcgacttgc cgccgcacgt
     2521 gatcctgccc gaggcgatgg gtccgaccgg cggcaatctg ccgcacgggc aggacgcggg
     2581 ctttttgggc aaggcccacg acccgttcgt cttgaacacc gatccgagcg tcgcgaattt
     2641 caaagtgccc gatctcttgc cgccgaagga gctgggcgac gtgcgcttgg agcggcggcg
     2701 gaagctgcgg tcgatcgtcg agcagcacgc ggccgagttc gaagccagcg ccagcgcgaa
     2761 gctgatggac gcgaacttcg aggcggcctg gcggatgatg accagcccgc aggcccgcgc
     2821 ggcgttcgac ttgtcgcagg agccggactc cgtccgcgac aagtacggcc gcaaccgctt
     2881 tggccagtgt tgtttgctcg ctcgccgcct gatcgaggcg ggcgtgcggt tcgtgacggt
     2941 caacacgttc ctcaccgtct tcgacgagat cacctgggat atccacggca gcaagccctt
     3001 caccagcatc gaagggatga agaacatcgt cgcgccgatg tacgaccagg cctacggcac
     3061 gctgatcgag gacctggccg accgcggcct cttggccaag acgctggtct gcaacctggc
     3121 cgagttcggc cgcacgccgc ggatcaatcc cgccggcggc cgcgaccact ggccgcagtg
     3181 cttctgtgcg tatttcgccg gcggcggcat tcagggcggc cgggccgttg gcaagagcga
     3241 cgccatcggc gcgtatcccg ccgagcgggc cgtcgagccg gcggaagtcg tcgccacgat
     3301 ctacaagagt ctgggcttcg acctcgacac cacgctcccc ggccccgccg gccgcccctt
     3361 cccgctcgtc gatttcggca agcacgagat caaagagttg ttctagccgc ggtgcagcgt
     3421 catgagtgac gggccgcgaa tgaatcgtcc cagcgtggcc gtcctgacgc cgcagggccg
     3481 tggggccgtg gcgacgattg gcatccgcgg cgccggcgcg gtgcagctcg tcgatcaatg
     3541 tgttgccctg gcagcgggcc aaccgctggg ctcgtgcgcg gccggccgcg tgctgctggg
     3601 ccgcgttgga ttcgcaggtt ccgcagccga ggaagtggtc gtcgggctcg tcgcgccgga
     3661 cgaagtcgag gtgcattgcc acggcggcct ggcggtggcg gaggccgtcg cccaggcact
     3721 cgtggcggcc ggtggccggc gagttggctg gcaggagtgg gttcaggatg gcgcacccga
     3781 cccgattgcg gctgcggccc tgctcgcctt gccgcaggct cgcacggagc ggaccgcggc
     3841 gatcctgctg gcccagtatc gcggagcgct gcggagcgaa gtcgttgcga tcgacgaact
     3901 gctgggccgc ggcgaaaact cggccgcggg caatcggttg acgcaactcg cggagcgggc
     3961 cgatgtcggc ctgcacctga cgcagccctg gcgcgtggtt gttgcggggc ggccgaacgc
     4021 cggcaagagc agcctcgtca acgccctgct cggctaccag cgggcgatcg tcttcgagca
     4081 gccgggcaca acccgcgacg tgctcaccgc cgccacggcg ctggacggat ggccgatcga
     4141 attggccgat acggcgggcc tccgtgctcc gggcaatccg attgaggcgg cgggcgtcga
     4201 acgagcagcc gctgcgatta ctgccgccga cctgacgctg ctggttgccg aaacgacggc
     4261 cgcctgggac gcagcgctcc acgcacaagt cgcggccagc gcccgacggc tgctcatcgt
     4321 ccacaacaaa tccgacctgg ccccgccgcc tgccgatggc cgtccggcgg gaatcgcggt
     4381 cagcgccaaa acgctcgcgg gcatcgctgg gctcgccgcg gcgatcaccg cggcgcttgt
     4441 cccacagccg ccgacggtgg atgcggcggt tccatttacg gccgagcagt tcacggctct
     4501 tgctgaggcg cgcgagcagc tgactcgctc tggcgccggc gcagcccggt cggcgctcac
     4561 gcggctgctg gggaagttac aatgcccgcc atgaagccct ttttacccct gctgctcgcc
     4621 agcctgaccg ccacagcgct tgccgccgac ggctttcccc gcgtgccgcc gacgccgcca
     4681 gaaaaggccg aggcgacgtt cgaaacgctg cacggcttcc ggatggagct gatcgcggcc
     4741 gagccgctag tcgccagccc cgtcgatctg gcctacgacg aggacggccg ggcctacgtc
     4801 gtcgaaatgc gggactatcc gtatcccgaa gagaagaacg ccgtcccgac cgactttccc
     4861 ggcaccgtgc ggctgctgga agacgcggac cgcgacggca aatacgacaa ggcgacgatc
     4921 tttgccgacc agctcgcctg gccgacttcg gtctgctgct ataagggcgg cgtattcgtc
     4981 gcggcggccc cggacatctg gtatttcaag gacactgacg gcgacggcaa ggccgacgtg
     5041 cggcgcaaag tcttcacggg cttcagccgc tacaacgtgc aggcgattat gaacagcctc
     5101 cgctgggggt tggacaacaa gatttacggg tcggcctcgg gcaacggcgg caatatccgc
     5161 cacgcggagc ggccggatga tccgctgatt caactgaccc gacgcgactt ccgctttgat
     5221 ccggtcacgg agaagatcga agcgatcagc ggcggcgagc ggtttggggc gacgttcgac
     5281 gactggggca accgctttct gtgcaacatt cgcaacccgg tgcagcacgt cgtcctgccg
     5341 ctgcattatc tggcccgcaa tccgaacctg gtcgtgccga gcgcgatcca cgactgcgcc
     5401 gaatcgggcg atcagctgcg cgtctatcgc ctcagcccgc cggaagcctg gcgcgagttc
     5461 cgcgcccagc ggtgggccct ggagcaggcc aacatgccgc ggagcgagac gatcggcgcg
     5521 ggcttctgga ccagctccag cggcgtctgc gtctatcgcg gcgcggctta tcccgaaccg
     5581 ttccgtggca acgtctttat cggcgaagtc gccggcaacc tcatccaccg ccaaatcctc
     5641 gagcgcgatg gcgtcacgtt caaaagtcgc cgcgccgacg agaacac
//
LOCUS       DASRJB010000472         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1822071, whole genome shotgun sequence.
ACCESSION   DASRJB010000472 DASRJB010000000
VERSION     DASRJB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822071"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1406
                     /locus_tag="VFB80_12425"
     CDS             270..1406
                     /locus_tag="VFB80_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZN34621.1"
                     /translation="MTIVDRYLLRLYVKVLVVCLLCLFGLFVVIDAFGNLDEFLAYGK
                     RHSAGVFGVLLDYYWPRLLWFFDRTGAMLAMVSVAFVLTLIQRSNELTAIVAAGISPA
                     RVARPLLVASLIVAILGIVNREFGLPLVRESLAKNAQDWLGEESRKCTPRYDLRTDIL
                     ISGKSTYSKDRRIAAPLFRMPPELSQWGPQILADNAYYQPESAGRPAGYLLRGVRQPL
                     DLVQRKSVSPHGLPVLYSPADTPWLKPDECFVASVVTFEQLSVGGSWRQYLSSLELIA
                     GIRGQTIEPGADVRLMIHARVVQPLLDLSLVLLGIPLVLSRGNRNIFLAAGIGGLLVG
                     AMMILVLVCHAIGSNYLLDATLAAWLPLLIFGPVAYASARPLWD"
     gene            1493..3160
                     /locus_tag="VFB80_12430"
     CDS             1493..3160
                     /locus_tag="VFB80_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN34622.1"
                     /translation="MAKGPWVDGLTIGQVLAETARRHGDREALVFPQLGRRWTWRQFA
                     ADVDAAARGLVALEIKPGEHIALWATNVPQWVLLQFAAARMGAVLVNINPAYRPFELQ
                     YVLGQSDSVALFLVDRFKASNYFEMLREICPQIAAAEEGWVNCPRFPKLRNIVTLKGA
                     PPAGLMTWDAMVRAGEKVTPEELEIISRSLSPQQAINIQYTSGTTGFPKAATLSHRNL
                     LLNAYYVGACQRFTEQDRLCIPVPFYHCFGCVLGTLACAVYGTTMIVPAEYFQPGPTL
                     DALEKERATALYGVPTMYIAQLQDPSFAGRKISTLRTGIMSGAPCPIEVMRQVIDKLG
                     PTELTIAYGQTEASPVITQTRAEDPIELRVETVGRPLPDVEVKIVDPATGAELPDNQQ
                     GELCSRGHVVMLGYYNNPEATAAAIDAAGWLHTGDLAVRLPSGYYKITGRLKDMVIRG
                     GENIYPREIEEFLFTHPAVEQAAVFGVPDPKYGEELSAWIKLKAGCSATADEVRAYCK
                     SQLAHFKVPRYVKFVDSFPQTVTGKIQKFKMRDVMKEELGLVDQKTA"
BASE COUNT          590 a         1074 c         1110 g          625 t
ORIGIN      
        1 tcgcactcgg ggcaaaagag cggctggcca gcgcgcgaca ggcactcggc cgcacagaac
       61 gtgcagcgtg gcggaaacaa gaggtccagc gccgccctgc ggctggcagg cagccagcgc
      121 cagacgcggc gcggcggcag ctgggccgtc ggtcctgcta ctgtgtctgt cacgctagcg
      181 aggcttgctt tcgtcttgac cctgagtgcc aaagcggcct atcgtaccgc cgcgaagggc
      241 aagccgccat tgtttcggaa cggactccga tgacgatcgt cgatcgctac ctgctgcggc
      301 tgtatgtcaa ggtgctggtg gtctgtctgc tctgcctgtt tgggctgttc gtcgtcatcg
      361 acgccttcgg caacctggac gagtttctgg cctacggcaa gcggcacagc gccggcgtgt
      421 tcggcgtgct gctcgactat tactggccgc ggctgctgtg gttcttcgat cgcacgggcg
      481 caatgctggc gatggtctcg gtagcatttg tgctgacgct gatccagcgg agcaacgagc
      541 tgacggcgat cgtcgccgcg ggcatctccc cggcccgcgt ggcgcggccg ctgctcgtcg
      601 cgtcgctgat cgtggcgata ctgggaatcg tgaatcgcga attcggcctg cctctagtcc
      661 gggagagcct ggcgaaaaac gcccaggact ggctgggtga agagtcgcgc aagtgtacgc
      721 cgcggtacga cctgcgcacc gacatcctca tcagcggaaa atcgacctac agcaaggacc
      781 gccgcatcgc cgcgccgctg tttcggatgc cgcccgagct gtcccagtgg gggccgcaga
      841 ttctcgccga caacgcctac taccaaccag aatctgccgg ccgacctgcc ggttatctgc
      901 tgcgcggcgt gcggcagccg ctagacctgg tgcagcggaa aagcgtctcg ccgcacggac
      961 tgccggtcct gtactcgcca gccgatacgc cctggctcaa gcccgacgaa tgcttcgtgg
     1021 ccagcgtcgt cacgttcgag cagctctccg tcggcggctc ctggcggcaa tatctctcgt
     1081 ccctggagct gatcgcgggc atccgcggcc agacgatcga gcccggggct gacgtgcggc
     1141 tgatgatcca cgcccgcgtc gtgcagccgc tcctggacct gtcgctggtg ctgctgggaa
     1201 ttcccttggt tttgagccgc ggcaatcgta atattttcct ggcagcgggg atcggcgggc
     1261 tgctggttgg ggcgatgatg attctggtgc tggtctgcca cgcgatcggg tcgaattact
     1321 tgctcgacgc gacgctggcg gcgtggctgc cgctgctcat cttcggcccg gtggcctatg
     1381 cgtccgcccg cccgctgtgg gattagccgt agcataagcg tcctcgcttg tgcagcgccc
     1441 ttcatggagg cgtgagcaag cgaggacgct tgctctacga ggagaccggg cgatggcgaa
     1501 aggtccctgg gtggacggac tgacgattgg ccaggtgctg gcggaaacgg cgcggcggca
     1561 cggcgaccgc gaggcgctcg tctttccaca gctcggccgc cgctggacct ggcggcaatt
     1621 cgcggccgac gtcgatgccg cagcccgcgg cctggtggcg ctggaaatca aaccgggcga
     1681 gcacatcgcg ctgtgggcga cgaacgtgcc gcagtgggtg ctgctgcaat tcgccgcggc
     1741 ccgcatgggg gcggtgctcg tcaatatcaa cccggcctac cggccatttg aattgcagta
     1801 cgtgctcggg cagagcgatt cggtggcgct gttcctcgtc gatcggttca aggcgtcgaa
     1861 ctatttcgag atgctccgcg aaatctgccc ccagatcgcc gccgccgagg agggctgggt
     1921 caattgcccg cgctttccca agctccgcaa catcgtcacg ctcaagggcg cgccgccggc
     1981 cggcctgatg acctgggacg cgatggtccg cgcgggcgag aaggttacgc ctgaagagct
     2041 ggagatcatc agccgctccc tctcgccgca gcaggcaatc aacattcagt acacgtcggg
     2101 gacgaccggc ttcccgaagg cagcgacgct cagccatcgc aatttgctgc tgaacgcgta
     2161 ctacgtcggc gcgtgccagc ggtttaccga gcaggaccgg ctctgcattc ccgtgccgtt
     2221 ctaccactgt tttggctgtg tgctggggac gctggcctgt gcggtctatg gcaccacgat
     2281 gattgtgccg gcggagtact ttcagccggg accgacgctc gacgcgctgg agaaagagcg
     2341 ggccactgcg ctctatggcg tgccgacgat gtacatcgcc cagcttcagg acccgtcgtt
     2401 cgccggccgg aagatcagca cgctgcggac gggcatcatg tcaggcgccc cctgcccgat
     2461 cgaagtgatg cggcaagtga tcgacaagct cggcccgacg gagttgacga tcgcctacgg
     2521 ccagaccgag gcctcgccgg tcatcacgca gacgcgggcc gaggacccga tcgagctgcg
     2581 cgtcgagacc gtcggccgcc cgctgccgga cgtcgaggta aagatcgtcg atccggccac
     2641 cggcgcggaa ttgcccgaca accagcaggg ggagctctgc agtcgcgggc acgtggtgat
     2701 gctcggctac tacaacaacc ccgaggccac ggcggctgcc atcgacgccg caggctggct
     2761 gcacacgggc gacctcgccg tgcggctgcc cagcggctat tacaagatca cgggccgcct
     2821 gaaggacatg gtcatccgcg gcggcgagaa catctatccc cgcgagatcg aagagttcct
     2881 gttcacgcac cctgccgtcg agcaagccgc cgtgttcggc gtgcccgatc ccaagtatgg
     2941 cgaagagctg tcggcctgga tcaagctcaa ggctggctgt tccgcgacag ccgacgaagt
     3001 ccgagcctac tgcaaatcgc agctcgctca cttcaaagtg ccgcggtacg tcaagttcgt
     3061 cgattccttt ccgcagaccg tgaccggcaa gatccagaaa ttcaaaatgc gcgatgtgat
     3121 gaaagaagaa ttggggctgg tggaccagaa gacggcgtga ctgccgcgcc tgatcgtgca
     3181 tcgacgttgg ctcggcggag gcgctaagcc agaaatattt atttagtgaa cggaataata
     3241 gaaatcgctc gcgcatctaa gttatgggtg cgacctcgat gcggtggttg acacctggcc
     3301 ttcgaacccg gctcgcgtta gcgacctgtt caccccgttt ctctaagcct tatgatgcta
     3361 atgtgaacaa atcgccataa atcgatattc agcaatagg
//
LOCUS       DASRJB010000473         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1822129, whole genome shotgun sequence.
ACCESSION   DASRJB010000473 DASRJB010000000
VERSION     DASRJB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822129"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFB80_12435"
     CDS             <1..686
                     /locus_tag="VFB80_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN34623.1"
                     /translation="AICCVLLFLTGLFRTLPDAVLAAVVLVAVSGLIDIPELRHLWRA
                     SRLDFAAAVVALAGVLLMGVLDGVLIAVLASILMLLARMSRPAVAFLGRIPGTNRFSD
                     LARHPSNEAIGGLLAFRVQSSLLYFNVDHVLEIVRQHAHAQPGLRRVICDLSNVPYMD
                     VAGARMLRRLHAELVRENIDLKIVEAHGPVREALRAERLEELVGPITRGASLAEAVAE
                     HEAPPKSAG"
     gene            1058..>2129
                     /locus_tag="VFB80_12440"
     CDS             1058..>2129
                     /locus_tag="VFB80_12440"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN34624.1"
                     /translation="MRGRVDTDAVWTTQSPASLATFGDLGDVVGLRRARIGVQGDLAI
                     GGNYLAEIDLASGDVVIRDLFVGLGEVRGGGEFRSGHYREPFSLEIDTGANYFAFLER
                     SPVSVLDPARNWGLGLLRAGESSTFALGVFQSGTDANDFEGGPGSTVDLTGKWTAAPI
                     NEGEGERLLHLGLAFSERFPEDGVIIIQEQTQSPLLDLGDVATSPFVPRISVPASFQQ
                     LLNLQFAAARGPFWTQAEWYGSWIDQLGGGTVYLYGCHADCGWFLTGEHREYRAASSS
                     FGPIRVSRPLIRCHACDRPAGWGAWELTARYSYLDFQDPDTPPGPGGQLVGILLPEAT
                     FGVNWYLCDRIRLMFNYTYALPN"
BASE COUNT          303 a          735 c          692 g          399 t
ORIGIN      
        1 cggcgatctg ctgcgtgctg ctatttctga ccggtctctt ccgcactctg ccagatgccg
       61 tgctcgcggc ggtcgtgctc gtcgccgtgt ccgggctgat cgacattccc gaactccgcc
      121 acctgtggcg ggccagccgg ctggacttcg ccgccgcggt agtggccctg gccggcgtgc
      181 tgttgatggg cgtcctcgac ggcgtgctga ttgccgtgtt ggcttcgatt ctgatgctgc
      241 tggcccgcat gtcgcggccg gcggtcgcct tcctcgggcg aattcccggc accaatcgct
      301 tttccgacct ggcccggcac cccagtaacg aggcgatcgg cggcttgctc gcttttcgcg
      361 tgcagtcctc gctgctctac ttcaacgtcg atcacgtcct cgaaatcgtg cggcagcatg
      421 ctcacgcaca accgggcctg cggcgcgtca tctgcgatct gtcgaacgtt ccctacatgg
      481 acgtcgccgg cgcgcggatg ctgcggcggc tgcacgccga actggtgcgg gaaaatatcg
      541 acctcaagat cgtggaggcc cacggcccgg tccgcgaggc gctgcgggcc gaaaggctcg
      601 aagagctcgt cggcccgatc acgcgcggcg caagcctggc cgaagctgtc gccgagcatg
      661 aagcgccgcc caaatctgca ggctagggaa gccttgccgc tcaatccgga tttgccggca
      721 atctcctacg gcacgcggcg cacttgcggc tggcgagaag tctcaagcga cagagcctgc
      781 acagcttgcc cagggtgcaa tctctgcccg ttcctcgttt tcgaagtcga ggaggttcga
      841 ttttctggat gtgcgatact ctgctttcgc tgccgcggga aggggcctgg cgacctggct
      901 actggtcgcc tgggcgtgct gcttcgtccc tgcgttggcc tgcgtccgcg cggaagacct
      961 ggccccgctc ccaagcgccc cagggataga ggccgaaatc tgtcccgatg gcacgtgtcc
     1021 gccccagtcc gctgacgttg gcagaacgac cttccgcttg cggggccgcg tcgataccga
     1081 cgcggtctgg acgacgcaga gcccggccag cctcgcgacc ttcggcgacc tgggcgacgt
     1141 cgtcggcctg cggcgggcgc ggatcggcgt ccagggcgat ctggccatcg gcggcaacta
     1201 tctcgcggaa atcgacctgg cctccggcga cgtcgtgatc cgcgatctgt ttgtcgggct
     1261 cggcgaagtt cgcggcggcg gcgaattccg ctccggccac taccgcgagc cgttcagcct
     1321 ggagatcgac accggagcga actatttcgc gttcctggag cgctctccgg tcagcgtcct
     1381 cgacccggcg cggaattggg gcctggggct gctgcgtgcg ggcgagtcaa gcacgtttgc
     1441 gctgggcgtc tttcaatcgg gcacggatgc gaacgacttt gagggaggcc caggcagcac
     1501 cgttgacctc acgggaaaat ggacggccgc tcccatcaac gaaggcgagg gcgagcgctt
     1561 gctgcacctg ggcctggcgt tttccgagcg gttccccgag gacggagtca tcatcatcca
     1621 ggagcagacg caatcgccgc tgctggacct gggcgacgtg gccacctccc ccttcgtgcc
     1681 gcgcatcagc gtcccggcca gcttccagca gctgctgaat ctccagttcg cggcggctcg
     1741 cgggccgttt tggacgcaag ccgaatggta cggctcctgg atcgaccagc tcggcggcgg
     1801 gaccgtctat ctgtacggct gccacgccga ttgcggctgg ttcctcaccg gcgaacaccg
     1861 tgagtatcgg gctgcgagca gctctttcgg cccgatacgt gtcagccgcc cgctgattcg
     1921 ctgccacgcc tgcgatcgtc cggccggctg gggcgcctgg gagctgaccg cccgctattc
     1981 ctacctcgac ttccaggacc ccgacacgcc gcccggcccc ggcggccagc tcgtcggcat
     2041 cctcctgccc gaagcgacgt tcggcgtgaa ctggtatctc tgcgaccgca tccgcctgat
     2101 gttcaactac acgtacgccc tgcccaacg
//
LOCUS       DASRJB010000474        13227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1257075, whole genome shotgun sequence.
ACCESSION   DASRJB010000474 DASRJB010000000
VERSION     DASRJB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13227
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257075"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VFB80_12445"
     CDS             <1..2181
                     /locus_tag="VFB80_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN34625.1"
                     /translation="AICASAAAAEQASFDRDVMAVLSKAGCNLGACHGNANGKGGFKL
                     SLRGQDPAVDYLSLTRDAEQRRVNLLDTEASLILLKPTAQVVHQGGLRFKRDSQEYRL
                     LLDWIAAGAPGPSPEGSQLEKLEVTPAKAIVMSPDEQVQLMAIAHFAGGKSRDVTALA
                     CYEPTNRSVTISPGGLVRREKLGQATVIVRYLDKQLAVPLAFLPARPDFVASSPPPAN
                     YIDELVFAQLRELRMNPSPTCDDATFIRRATLDAVGLLPTAAEARAFVADTASEKRTK
                     LIDQLLARPEFAEHWALKWSDLLRNEEKVLDAKGVDTFYGWIRDSIAAGKPIDRFVRE
                     LVSARGSTYENPPANFYRANRDPATRGETAARLFLGVRLQCARCHTHPYDRWTQDDYY
                     SWAAVFGRIDYQILSNTRKDRLDKHEFNGEQLVLIAEEGEVTNARTGLDAPPKFLGAY
                     SPSLHPAGDRLEPLADWLASRENQLFVNSQANFIWYHLLGRGLVEPIDDFRVTNPASI
                     PPLMEALARDFARCGFDLRHLVRAIMNSRTYQAAAEPNETNADDEGCFSRAIVRRLSA
                     ESLLDAQCQVLGTRPEFHGYDAGTRAGQVRGTQRVRPRDRRPTAAERFLKTFGKPERL
                     LACECERSDGTTLKQAFTLIGDEGLNEMLEGEQNRLAELAGSARPSAEIIAELYWSAL
                     SRGPAPDELSAAEALLASGGENRLSPLQDLAWAILNAKEFVFRH"
     gene            2193..3278
                     /locus_tag="VFB80_12450"
     CDS             2193..3278
                     /locus_tag="VFB80_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34626.1"
                     /translation="MFGKNWRGVRPARVQRRPRLALERLEDRSLMAAFADLVAYRPVT
                     EYINYANYPVAAAVETDPKAGPGIRVNGDDDNANGVADFSDAAATAAENDLVRVDVNA
                     SGGDFTLSWPDELRLWTTSTKTQAVTEGALYLAGPRTWWVEYVGAAHSSAATLSLIVG
                     AGADVAQDDVTFHSFQSDVIVIGGNSQNPANVGDPRLGVFTTGLTLYKQGYDVHLYSH
                     DKVQSTGQGAAFNEVKSAVLKRNVDYVAILGYSWGGGATYELAAGLKADASLAGQFQL
                     KYTASIDGIRHNTISAETRLPPGTLYHDNLFQRRDWLLRGNNVAGAVNLNVTSTAWGQ
                     SLVHTTIDDSAMVQSIIVSNLTTKVPA"
     gene            3311..4339
                     /locus_tag="VFB80_12455"
     CDS             3311..4339
                     /locus_tag="VFB80_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN34627.1"
                     /translation="MRHTLAPIASLLLCTAALAGPADQQLDIYWIDVEGGAATLLVTP
                     AGETVLIDTGNPGRRDPDRIVKAVTAAGMKQIDHLIVTHYHGDHFGGALTLVDQLPVK
                     NMYDNGQFEGLTENPGKAYFELKVEKRHVVKPGLTLPLAQADRAAKISFTFVGGAKKF
                     VDAPAGAADNADLCASGREKERDLSDNANSVVMLVQFGPWRFYDAGDLTWNQEARLVC
                     PKNLIGQVDVYQVTHHGLDSSNNPLVLRSLQPRVAIMNNGSTKGCLPEVASNLKETKS
                     LEAVYQLHKNLRPDGATGNVADEYIANKEKDCQGNYVKLSVAADGKSYTVAIPANKHE
                     RKFQTRTN"
     gene            4460..5836
                     /locus_tag="VFB80_12460"
     CDS             4460..5836
                     /locus_tag="VFB80_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34628.1"
                     /translation="MSQLVFPRRTVLRAGGAGLLGLTLPGLLRAADSAKAQPAGLKVR
                     AKRVIFLFQWGGPSHVDMFDMKPKAPDDYRSPLAPIASSCPEIQVCEQLPETARRMHH
                     VTLIRTLTHAMKNHNSAGYYALSGHAPPTDDQRLRDSLDLFPGYGSVVDKLAPSKNGM
                     PTFVSYPYTIADGSITPGQHASFLGKAHDPLFFTEDPAASDFRLPELSLPDNLPLDRL
                     HRRREMQRLVDRQARLLEYSAEARGFDEYYDRAIGMLTSDKVRQAFDLSTEPAEIRDR
                     YGRSTYGQSCLLARRLVEAGVKFVTVYFAASIGGRSDTSGGWDTHGFDNTRMFPIVKK
                     YHFPRTEETLPTLLDDLSERGLLDDTLVLWMGEFGRTPKINANVSRDHWPQCYTALLA
                     GGGVKRGFVYGKSDATGSYPDEHPVRPDDLAATLYYLMGINPEAEIHDRTDRPLVIGG
                     KPVLEVVA"
     gene            5836..6645
                     /gene="proC"
                     /locus_tag="VFB80_12465"
     CDS             5836..6645
                     /gene="proC"
                     /locus_tag="VFB80_12465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZN34629.1"
                     /translation="MLTQTIGFIGAGQMARALGQGFQVAELIPGERIVAWDPVPEAGQ
                     AFTQVVRGAVLAASNAEVVRRADVLFLAVKPQSMPAVMHELAGKIGSDKLVVSVAAGI
                     TIGRLSEGLKTGRVVRVMPNTPALVGQGASAYALGPGATAADGQLVGKLVGAVGRAFA
                     VDEKLLDAVTGLSGSGPAFVYVMIEALADGGVRMGLPREIAAALAAQTVRGAAEMVIA
                     TGEHPAVLKDKVASPGGTTIAGLQALEDQGLRAALIAAVAAATRRSQELGT"
     gene            6799..7062
                     /locus_tag="VFB80_12470"
     CDS             6799..7062
                     /locus_tag="VFB80_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34630.1"
                     /translation="MPSPPLFVVVDDKHVPLFRILWISDVPHFCGNDDCECEGDYEVR
                     LEQDESVWASRAERDKVLKAIEEWYNGEHDHDHGWEEGETEGE"
     gene            7177..7665
                     /locus_tag="VFB80_12475"
     CDS             7177..7665
                     /locus_tag="VFB80_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34631.1"
                     /translation="MSLFADSRYQWRETYFVLFDRQRRPLAADVKRALVELGPRIEVH
                     EINASESGQLESMTILSHADAAGMDITYVEGEEVKEQIVQLRQEWKGKRPGEKEKPHL
                     ARALHADARFDVFHFEEISTIPDEEDDGFLDPGTLLLVLNKLARLCHGEALDPQTGEL
                     LE"
     gene            complement(7733..8050)
                     /locus_tag="VFB80_12480"
     CDS             complement(7733..8050)
                     /locus_tag="VFB80_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HZN34632.1"
                     /translation="MAWIILAIAGVLEITWAVCLKKSEGFTRPPWGAISLVLSLASVI
                     LLALALRTIPLGTGYAIWTGIGAAGTALLGIWLFQEAASPLRLLFIALIVISMIGLRL
                     TAR"
     gene            complement(8071..9432)
                     /locus_tag="VFB80_12485"
     CDS             complement(8071..9432)
                     /locus_tag="VFB80_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HZN34633.1"
                     /translation="MSDTLAVRPDAEPAARRSDSSSISPPDASGHPALHERLLEPLAA
                     RLKRHKITRIGLEFLDRCADFHLKDLRIDDMDDHRGASILGRRVVNFGSDSFLGLDRD
                     ERVQKAIVESLPQWGTHNGASRAFSSVALCTEAEARLARWLGVEDTLIFPSVTLANAG
                     LIPALASKGDLLVVDRQSHDSIHQATKIASSNGARVVEMHPCRGDVLESLLSSHEGGK
                     GSITAVDGVYSMSGKVSPLAELNEVARRHGGFLYIDDAHGTGVVGPRGRGAASVALPS
                     LRGVLQVGSLSKGFSCLGAYVTCHPELKRILKIRSSTFIFGGPVPPPYLAAIIAVCDI
                     LDSPEGDELIAHLRSLVVRLTEGLNRLDLIVAGGVAPIVSVQVGEIEETFAAGKWLFD
                     RGYYVQSATYPAVGINEGLIRIQVNANHRPEAIDGLIAAFADLKREHRLPAASAQRPR
                     AVL"
     gene            9546..10709
                     /locus_tag="VFB80_12490"
     CDS             9546..10709
                     /locus_tag="VFB80_12490"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZN34634.1"
                     /translation="MPPTPADRSSHPGWTEAAPWMGCSLPALVRILAGGRFRVDLASW
                     PECLVDLVFASGNSAAGLVQKVIFGGRAARTELTADPVFVIGHWRTGTTLLHELLALD
                     PRLRAPTTYECLAPHHFLLTGSWLPRLTTFALPKTRPPDKMRVAWDAPQEDEFALVNL
                     GVPSPYATIAFPNDGSANDAWLELDTLPTPDRQRWERAVVAFFRQLTYARPGRLLLKS
                     PTHTFRLPTLTRLFPQARWINIARNPYEVFSSTVRLWRSMYQAYGYQRPRLTGLEEFV
                     LATFERMHERLERTRPLAPAGGIVDVRFEDLLAAPVETMRGVYAALELGDFAVVEPKV
                     AEYFAARASFAANRHELEPRWQEEVSRRWLPYFERYGYSLAAKSARPANPAHA"
     gene            10766..11701
                     /locus_tag="VFB80_12495"
     CDS             10766..11701
                     /locus_tag="VFB80_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34635.1"
                     /translation="MSTQPQISPLAVFNGSFLGLIALIIGFATSFIAGHELLTSLRCG
                     ASPSPASVAELAQHGPTAGDYVRLNDFEVEWQGYIYWPGEDGRWTFCDVPLRVAGSAA
                     PPRVLARVYGAANDAELRRLMAGSELTGIVSGHGIRGEGAAALASYNPGMDPGACWIV
                     SINRHPADSRLLGGIFAAGVGIFTLSMFLFVYCRPRATPEQAVLKMMSPLLIMVEGLH
                     ALSDWLPAGSRRICGAVLFAPGAALAAWGGYGLWQAARASAAEVGAGPEILAILALDF
                     GVALALVALSFVFVEPKGEAPASAVYPQASAGAGA"
     gene            complement(11719..12795)
                     /locus_tag="VFB80_12500"
     CDS             complement(11719..12795)
                     /locus_tag="VFB80_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HZN34636.1"
                     /translation="MSIDLRSDTVTRPSPEMRLAMANADVNDDVIDVDPTCDRLQKLT
                     AEILGKEAAIFMSSGSQTNQVGVRVHCKPGDEFICETGCHIYNYEQGAFAQLSGIVAR
                     TVDGKAGVLHVDQLRGLVRPENDHLVRTRLVCLENTHNRGAGKIQPLENVVGICRWAH
                     DSGLVTHLDGARLWNAAAATGVPEAEWCKHFDTVSVCFSKGLGAPVGSALAGPKDLIK
                     EARRHRKLFGGAMRQVGVIAAGALYALQNNRARLSQDHANAQILAESIRAAEGLSLMP
                     EAVETNIVIFRVEPKLGSAAEFAAELKSQNVLCLAIGPQQVRLVTHLDVSEAQCRQAA
                     KLIRETAGRLASGQKSAKELEPAY"
     gene            complement(12818..13099)
                     /locus_tag="VFB80_12505"
     CDS             complement(12818..13099)
                     /locus_tag="VFB80_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34637.1"
                     /translation="MTANSAGYSAHVEMFLILNGQQFDLGQIGPEHCIVRNPAEVPPS
                     PGEIVLIVDGNETRLQVYLPHGISSSSRKVAYRPASPAAAPWPAAAASR"
BASE COUNT         2294 a         4335 c         4389 g         2209 t
ORIGIN      
        1 gcgatttgcg cttcagcggc agcggctgag caggcgtcgt ttgaccggga cgtgatggcg
       61 gttctgtcga aggcgggctg caacctgggg gcgtgtcacg gcaacgccaa cggcaaaggg
      121 ggcttcaagc tctcgctccg cgggcaggat ccggcggtgg attacctgtc gctgacgcgc
      181 gacgccgagc agcggcgggt gaatctgctc gatacggaag cgagcctgat cctgctgaag
      241 cccacggccc aggtcgtgca tcaaggaggc ctgcggttca aacgcgattc gcaggaatac
      301 cggctgctct tggattggat cgccgccgga gcgccggggc cgagcccaga gggctcgcag
      361 ctggaaaagc tggaagttac tccggccaaa gcgatcgtga tgtcgcccga cgagcaagtg
      421 cagctgatgg ccatcgccca cttcgccggc ggcaagtcgc gcgacgtgac ggcgctggcc
      481 tgttacgagc cgacgaaccg cagcgtgacc atcagtccgg gcggcctggt gcggcgcgag
      541 aagctgggcc aagcgacggt gatcgtccgc tatctcgaca agcagctcgc ggtgccgctg
      601 gcgtttctgc ccgcgcggcc cgatttcgtc gccagttcgc cgccgccggc caattacatc
      661 gacgagctgg tcttcgccca gctgcgtgag ctgcggatga acccctcgcc gacctgtgac
      721 gacgcgacgt tcattcgccg ggccacgctc gacgccgtcg gcctcttgcc gacggccgcc
      781 gaggcccggg cgttcgtcgc cgacaccgcc agcgagaagc ggacgaaact catcgaccag
      841 ctgctggccc ggccggagtt tgctgagcac tgggcgctca aatggtcgga cctgctccgc
      901 aacgaagaga aggtgctcga cgccaagggg gtcgatacgt tttacggctg gattcgcgac
      961 tcgatcgcgg cagggaaacc gatcgaccgc tttgtccgcg agctggtctc ggcccgcggc
     1021 agcacgtacg agaacccgcc cgccaacttc taccgggcca atcgcgaccc ggccacccgc
     1081 ggcgagacgg ccgcccggct gttcctcggc gtgcggctgc aatgcgcccg ctgtcacacg
     1141 catccctacg accgctggac gcaggacgac tactacagct gggccgcggt gttcggccgg
     1201 atcgattacc agattctcag caatacgcgc aaggaccggc tcgacaagca cgagttcaac
     1261 ggcgagcaac tggtgctgat cgccgaggaa ggcgaggtta ccaacgcccg cacgggactg
     1321 gacgccccgc ccaagttcct gggggcctac tcgccgtcgc tgcatccggc cggcgaccgc
     1381 ctggagccgc tggccgattg gctggcctcg cgggagaacc agttgttcgt gaattcgcag
     1441 gcgaatttca tctggtatca cctgctcggc cgcggcctgg tcgagccgat cgacgacttc
     1501 cgcgtcacca acccggccag cattccgcca ctgatggagg ccctggcccg cgactttgcc
     1561 cgctgtggct tcgacttgcg gcatctggtc cgcgcgatca tgaacagccg cacgtaccag
     1621 gcggccgcgg agccgaacga gacgaacgcg gacgacgagg gctgcttttc gcgggcgatc
     1681 gtccgccgcc tcagcgccga gtcgctgctc gacgcgcagt gccaggtgct gggcactcgg
     1741 ccggaatttc acggctacga cgccggcacg cgggccggcc aggtccgcgg cacgcagcgc
     1801 gtgcggcccc gcgaccgcag gcccacggcc gccgagcggt tcctcaagac atttggcaag
     1861 cctgagcggc tgctggcctg cgaatgcgaa cggtccgacg gcacgacgct aaagcaggcc
     1921 ttcacgctga tcggcgacga aggactgaac gagatgctcg agggcgagca gaaccggctg
     1981 gccgagctcg cgggctccgc gcggcccagc gcggagatca tcgctgagct ctactggtcc
     2041 gcccttagcc gcgggccagc gcccgacgag ctgtccgctg ccgaagcgct attggcctct
     2101 ggcggcgaga atcggctgag cccgctgcag gacctggcct gggccattct gaacgcgaag
     2161 gaatttgtgt ttcgccacta ggaggagcaa ccatgttcgg gaagaactgg cggggcgtgc
     2221 gtccggcacg agtgcagcgt cggccaaggc tggcgctgga gcggctggag gaccgctcgc
     2281 tgatggcggc tttcgcggac ctggtcgcct atcggccggt gaccgagtac atcaactatg
     2341 ccaactatcc ggtggccgcc gccgttgaaa cggaccccaa ggccggaccg ggcattcgcg
     2401 tcaacggcga cgacgacaat gccaacggcg tggccgattt ttccgacgcc gcggccacgg
     2461 cggccgagaa cgacctggtc cgcgtcgatg tcaatgcctc ggggggcgac tttacactca
     2521 gctggcccga tgagctgcgg ctgtggacga cgagcaccaa gacgcaagcc gtcaccgagg
     2581 gtgcgctcta cctggcgggc ccgcgcacgt ggtgggtcga atatgtcggc gcggcacatt
     2641 cctccgccgc gacgctgtcg ctgatcgtgg gagcgggcgc ggacgtcgct caggacgacg
     2701 tgaccttcca cagctttcaa agcgacgtga tcgtcatcgg cgggaattcg cagaacccgg
     2761 ccaacgtggg cgacccgcgg ctgggcgttt tcacgacggg gctgaccctg tacaagcagg
     2821 gctacgacgt gcatctctac agccacgaca aggttcaatc gaccggccag ggcgcggcgt
     2881 tcaacgaggt gaagagcgcg gtgctgaagc gcaacgtcga ttacgtcgcg attcttggct
     2941 atagctgggg cggcggagcg acgtacgagt tggccgcggg cctgaaggcc gacgctagcc
     3001 tcgccggtca gttccagctg aaatacaccg catccatcga cggcatccgg cataacacga
     3061 tcagcgccga gacgcgcttg ccgcccggca ccctctatca cgacaatctg ttccagcgcc
     3121 gcgactggct gctccgcggc aataacgttg ctggagctgt caacttgaac gtcaccagca
     3181 cggcctgggg ccagagcctc gtgcatacca ccatcgacga cagcgcgatg gtgcagagca
     3241 tcattgtgag caacttgacc acgaaagtgc ctgcataatc acttcttcat ccttactgca
     3301 tccacaacac atgcgtcaca cgcttgcccc catcgcatcg ctcctgctgt gtaccgccgc
     3361 gctcgccggc ccggccgacc agcaactcga catttactgg atcgacgtcg aggggggcgc
     3421 ggcgacgctg ctggtgacgc ccgcgggcga gacggtcttg atcgacacgg gcaatccggg
     3481 ccgccgcgat cccgaccgga tcgtgaaggc ggtcaccgcc gccgggatga agcagattga
     3541 ccacctgatc gtcacgcact accacggcga ccactttggc ggcgcgctga cgctcgtcga
     3601 tcagctgccc gtcaagaata tgtacgacaa cgggcagttc gaagggctga ccgaaaaccc
     3661 gggcaaggcc tacttcgagc tgaaggtcga gaagcggcac gtcgtgaagc cgggcctgac
     3721 gctgccgctg gcgcaggcgg accgcgcggc gaagatcagc ttcacgtttg tcggtggcgc
     3781 gaagaagttc gtcgatgcgc cggccggcgc ggcggataac gccgacctct gcgcttccgg
     3841 ccgcgagaaa gagcgcgacc tctcggacaa cgccaacagc gtcgtgatgc tggtgcagtt
     3901 cggcccctgg cggttctacg acgccggcga cctgacctgg aaccaggagg cccggctggt
     3961 ctgccccaag aacctgatcg gccaggtcga tgtctaccag gtcacgcacc acggcctcga
     4021 ctccagcaac aatccgctcg tcctgcggag cttgcagccg cgggtcgcca tcatgaacaa
     4081 cggcagcacg aagggctgcc tgccggaagt cgcttcgaac ctgaaggaga cgaagtcgct
     4141 ggaagccgtc tatcagcttc acaagaacct ccgccccgac ggcgcgacgg gcaacgtcgc
     4201 cgacgaatac atcgccaaca aggaaaagga ctgccaggga aactacgtga agctctccgt
     4261 cgccgccgat ggaaagagct acaccgtggc gatcccggcc aacaagcatg agcggaagtt
     4321 ccagacgcgg accaactgat gcaggaaaca gaggaatcga acagaaggaa acgaaggtaa
     4381 cgaagaggag cttgcgtttc ttgattcttg tctgcttcgt ttgcttcgtt accttctgtt
     4441 caatctgcct ggtgagccta tgagccaact tgtttttccg cgccgtaccg tgctccgcgc
     4501 cggcggcgca ggtctgctcg gtttgacgct gccggggctg ctcagggccg cggatagtgc
     4561 gaaggcgcag ccggcggggc tgaaggtgcg ggccaagcgg gtgatcttcc tgtttcagtg
     4621 gggcgggccg agccacgtcg atatgttcga catgaagccg aaggcgccgg acgattaccg
     4681 ctcgccgctg gcgccgattg cgtcgagctg cccggaaatc caggtctgcg aacagctgcc
     4741 cgagacggcc aggcggatgc accacgtcac gctcatccgc acgctcacgc acgcgatgaa
     4801 gaaccacaac tcggccggct actacgccct ctccgggcac gccccgccga ccgacgacca
     4861 gcggctgcgg gactcgctcg acctgttccc gggctacggc agcgtcgtcg ataagctcgc
     4921 gccgtcgaaa aatggaatgc cgacgtttgt ctcgtatcct tacacgatcg ccgacggctc
     4981 gatcacgcca ggccagcatg ccagcttcct cggcaaggcc cacgacccgc tgttcttcac
     5041 ggaagacccc gccgccagcg attttcgcct gccggaattg agcctgccgg acaatctgcc
     5101 tctcgatcgg ctgcaccgcc gccgcgaaat gcagcggctg gtcgatcgcc aggcccggtt
     5161 gctggaatat tcggccgaag cccgcggttt tgacgaatac tacgaccggg cgattggcat
     5221 gctgaccagc gacaaggtcc gccaggcgtt cgacctctcg accgaacccg ccgagatcag
     5281 agaccgctac ggccgctcga cctatggcca gtcgtgcctc ctcgcccgcc gcctcgtcga
     5341 ggccggcgtc aaattcgtca ccgtctattt cgccgccagc atcggcggcc gcagcgacac
     5401 cagcggcggc tgggacacgc acggtttcga caacacgcgg atgttcccga tcgtcaaaaa
     5461 gtatcacttt ccccggaccg aagagacgct gcccacgctg ctcgacgacc tctccgagcg
     5521 cggccttctg gacgacacgc tcgtcctctg gatgggtgaa ttcggccgca cgcccaagat
     5581 caatgccaac gtcagccgcg accactggcc gcagtgctac acggcactgc tcgccggcgg
     5641 cggcgtcaag cggggcttcg tgtacggcaa gagcgacgcc accggctcgt accccgacga
     5701 gcatcccgtc aggcccgacg acctcgccgc cacgctctat tacctgatgg gcatcaaccc
     5761 cgaagccgaa attcacgacc gcaccgaccg ccccctcgtc atcggcggca aaccggtgct
     5821 ggaggtcgtg gcgtgatgtt aacgcagacg attggcttta tcggcgcggg gcagatggcg
     5881 cgggcgctgg ggcaggggtt tcaggtcgcg gagctcattc caggcgagcg aatcgtggcc
     5941 tgggatccgg tgcccgaggc ggggcaggcc ttcacgcagg tggtccgcgg cgcggtgctg
     6001 gcggccagca acgccgaggt ggtgcggcgg gcggacgtct tgtttctggc cgtcaagccg
     6061 cagagcatgc ccgcggtgat gcacgagctg gcgggcaaga tcggcagcga caagctcgtc
     6121 gtctcggttg ccgcggggat cacgatcggc cggctgagcg aagggctgaa gacgggccgc
     6181 gtcgttcgcg tgatgcccaa tacgcccgcg ctggtcgggc agggcgcctc ggcttatgcg
     6241 ctcggccctg gagcgacagc tgccgacggc cagctcgtgg gcaagctggt aggcgcggtc
     6301 ggccgcgcgt ttgccgtgga cgagaagctg ctcgatgccg tgaccggcct gtccggttcc
     6361 gggccggcgt tcgtctatgt gatgatcgag gccctggccg acggcggcgt gcggatgggc
     6421 ctgccccgcg agatcgccgc cgcgctggcc gcccagaccg tccgcggcgc ggccgaaatg
     6481 gtgatcgcca cgggcgagca ccccgccgtg ctgaaggaca aagtcgccag ccccggcggc
     6541 accaccatcg ccggcctgca agccctggaa gaccagggtc tccgcgccgc cctgatcgcc
     6601 gccgtcgccg ccgcgacccg gcgatcgcag gagctgggga cgtaatacaa acccgaagcg
     6661 tcagcgaggg gctttctagg cgcggcctgc gttcaaaaag tccctcgctc acgcttcggg
     6721 ttggtatcag ttgcctttac ccaaaaaccc gcccgcgacg tagaatagac gtagtttcgt
     6781 cgccccgggg attccgccat gccgtcaccg ccgctgttcg tcgttgtgga cgataagcac
     6841 gttccgctgt tccgcatcct ctggatttcc gacgtgcctc acttctgcgg caacgacgat
     6901 tgcgagtgcg aaggggatta cgaagtgcgg ctggagcagg acgaatcggt ctgggcctcg
     6961 cgggccgagc gcgacaaggt gctgaaggcc atcgaagagt ggtacaacgg cgagcacgac
     7021 cacgaccacg gctgggaaga gggcgagacg gagggggaat agcactaccg ccccggtatg
     7081 cacgcggtgt gaagattctc ggctgccggt ggtttacgcc gcgactgctt gctagattgc
     7141 ctgcaacttt cgccccgcac tcgccaaggg ctccgcatgt cgctctttgc cgacagccgc
     7201 tatcagtggc gcgaaacgta ttttgtgctc ttcgaccgtc agcgccggcc gctggccgcc
     7261 gacgtgaagc gggccctggt cgagttgggc ccgcggatcg aggtgcacga gatcaacgcc
     7321 agcgagagcg gccagctgga gtcgatgacg atcctgtcgc acgccgacgc ggctggcatg
     7381 gacattacct acgtcgaagg cgaagaagtc aaggaacaga tcgttcagct ccgccaggag
     7441 tggaaaggca agcggcccgg tgagaaagaa aagcctcacc tggcccgggc cctgcacgcc
     7501 gatgcccgct tcgacgtctt tcactttgaa gaaatcagca ccatccccga tgaagaagac
     7561 gacggattcc tcgaccccgg cactttgctc ctggtcctga acaagcttgc caggctgtgc
     7621 cacggcgaag ccctcgatcc gcaaaccggc gagctgctgg aataacggtc ggtcatagta
     7681 gtcatctcgc tccgcgagat gtttgcctca cgcggagcgt gaggtctact attcacctgg
     7741 ctgtcagccg caggccgatc atgctgatca caatgagcgc gatgaagagc agccgcagcg
     7801 gcgaggcggc ttcctgaaag agccagatgc cgagcagggc cgtgccggcc gcgccgatgc
     7861 cggtccagat ggcatagccg gtgcccagcg ggatcgttcg cagggcgagg gccagcagga
     7921 tgacgctggc caggctgagg acgagcgaga tcgcgcccca gggcgggcgc gtgaagccct
     7981 cgctcttttt caggcaaaca gcccaggtta tttccaggac gccggcgatt gccaggatga
     8041 tccaggccat gcgctgtttc tgcgtagggg tcataggacc gcgcggggcc gctgggccga
     8101 ggcggcgggc aagcgatgct cgcgcttgag atcggcaaaa gccgcgatca gcccgtcgat
     8161 cgcctccggg cggtggttgg cgttcacctg gatgcgaatc agcccctcgt tgatgccgac
     8221 ggccggatag gtagccgact gcacgtagta gccccggtcg aagagccatt tgccggcggc
     8281 aaaggtctct tcgatctcgc caacttggac cgagacaatc ggcgcgacgc cgccggccac
     8341 aatcagatca aggcgattca gcccctccgt cagccgcacg accagcgacc gcaggtgggc
     8401 gatcagctcg tccccttcgg gcgaatcgag gatgtcgcag acggcgatga tcgccgccag
     8461 gtagggcggc ggcaccggtc cgccgaagat gaacgtgctg gagcggattt tgaggatccg
     8521 cttcagctcg ggatggcagg tgacgtaggc ccccaggcac gagaatccct tggaaagcga
     8581 gccgacttgg agcacgcccc gcagcgacgg cagtgcgacg ctcgccgcgc cgcggccgcg
     8641 cgggccgacg acgccggtgc cgtgcgcgtc gtcgatatac aggaaaccgc cgtgccggcg
     8701 ggcgacctcg ttcagctcgg ccagcggcga gactttgccc gacatgctgt aaacgccgtc
     8761 caccgccgtg atcgagcctt tgccgccctc gtgggaggag agcagcgatt cgagcacgtc
     8821 gccccggcag ggatgcattt cgacgacccg tgctccgttg gacgacgcga tcttcgtggc
     8881 ctggtggata ctgtcgtgcg actgccgatc gaccacgagc aggtcgccct tcgacgccag
     8941 ggccggaatc aggccggcgt tggccagcgt aacgcttgga aatatcagcg tgtcctcaac
     9001 gcccagccag cgggccagcc gggcctcggc ttcggtgcac agggccacgc tggagaacgc
     9061 tcgcgacgcg ccgttgtgcg tgccccactg cggcagcgat tcgacaatcg ccttctgcac
     9121 gcgctcgtcg cggtccaggc cgaggaagct gtccgagccg aaattgacca cgcggcggcc
     9181 gaggatgctc gccccgcgat gatcgtccat gtcgtcgatc cgcaggtcct tgaggtggaa
     9241 gtcggcgcag cggtcgagaa attccaggcc gatccgcgtg atcttgtgcc gcttgagccg
     9301 cgcggccagc ggctcgagca ggcgttcgtg cagcgcgggg tgaccgctgg catccggcgg
     9361 cgaaatggaa gaagagtcgc tccgtctagc ggcaggctcg gcgtccggtc gaaccgcgag
     9421 agtgtcgctc atgagaggca gattctccaa aaccgacggc gggcccatca atgcgagatc
     9481 gtcactgtag cggcctccgc ggacagtgca agccgcgtgg cagccgctcg attagagtag
     9541 tacgcatgcc ccccacgcca gccgaccgca gctcgcatcc cggttggact gaggccgcgc
     9601 cgtggatggg ctgctcgctg ccggcgctcg tgcgaattct cgccggcggc cggtttcgcg
     9661 tggatttagc ctcgtggccc gagtgcctcg tcgatctggt gtttgcatct ggcaattcgg
     9721 ctgcaggact tgtccaaaaa gtaatcttcg gcggtagggc ggcccgcacc gagctgaccg
     9781 ccgatccggt ttttgtcatc ggtcattggc ggacgggcac gacgctgctg cacgagctct
     9841 tggccctcga tcctcggctg cgagcgccga cgacctacga gtgtctcgcg ccgcaccact
     9901 tcctgcttac cgggagctgg ctgccgcggc tgacgacgtt cgccctgcca aaaacccgcc
     9961 cgcccgacaa aatgcgggtc gcttgggacg cgccgcagga agacgagttc gcgctcgtca
    10021 acctcggcgt gcccagccct tatgcgacga ttgcctttcc gaacgacgga agtgccaacg
    10081 atgcctggct cgagctcgac acgctgccga cgcccgaccg ccagcgttgg gagcgtgcag
    10141 tcgtggcgtt tttccgccag ctgacctatg cccggcccgg ccggctgctg ctcaagtcgc
    10201 cgacgcacac gttccgcttg ccgacgctca cgcggctgtt tccccaggcc cgctggatca
    10261 acatcgcgcg caacccgtac gaggtctttt cctcgaccgt gcggctgtgg cgctcgatgt
    10321 atcaggccta cggctatcag cggccgcggc tgaccgggct ggaggagttt gtcctggcca
    10381 cgttcgagcg gatgcacgag cggctggagc gaacgcggcc cttggcgccg gccggcggca
    10441 tcgtcgatgt gcggtttgaa gacctgctgg ctgcgccggt cgaaaccatg cgcggagtct
    10501 atgcggcgct ggagctgggt gacttcgccg tcgtcgagcc gaaagtagcg gagtatttcg
    10561 ccgcccgcgc gagttttgca gccaaccgtc acgagctgga gccgcgttgg caggaagaag
    10621 tgagccgccg ctggctgccg tacttcgagc ggtatggcta tagcctggcg gcaaaatccg
    10681 cacggccggc aaatccggct cacgcttgag cagcacaatc gcggcctatt cttgcaggtc
    10741 ggccctgcca gcgccgggag aatctatgag cacgcaaccg cagatatcgc cgctcgccgt
    10801 tttcaacggc tcgtttctcg gtctgatcgc gctaatcatc ggctttgcaa cgtcctttat
    10861 cgccggccac gagctgctga cgtcgctgcg ctgcggcgcg tcgccttcgc cggcgagcgt
    10921 cgccgaactg gcccagcacg gtccgacggc cggcgactac gtccggctga acgatttcga
    10981 agtcgagtgg cagggctata tctattggcc cggcgaggac ggccggtgga ccttctgcga
    11041 cgtgccgctg cgggtggccg gttcggcggc cccgccgcgg gtgctggcgc gggtttacgg
    11101 cgcagccaac gacgccgagc tgcggcggct catggccggc agcgaattga ccggcatcgt
    11161 atccggccac gggattcgcg gcgaaggggc tgcggcgctg gcgtcctaca atccgggcat
    11221 ggatccgggg gcctgctgga tcgtctccat caatcgtcac cctgccgact cgcggctgct
    11281 gggcggcatc tttgccgccg gcgtgggaat cttcacgctc agcatgttcc tgttcgtcta
    11341 ttgccggccg cgggcgacgc cggagcaagc cgtcctgaag atgatgtcgc cgctgctgat
    11401 tatggtcgaa ggcctgcacg cgctctccga ttggctgccg gccggcagcc gccgcatttg
    11461 cggagcggta ttgttcgcgc cgggagcggc gcttgcggcg tggggcggat atgggctgtg
    11521 gcaagccgcc cgggccagcg cggccgaagt gggggccggc ccggagatcc tggcgatctt
    11581 ggcgctggac tttggcgtgg ccctagcgct cgtcgccctc agcttcgtgt ttgtcgagcc
    11641 gaagggcgaa gcgccagcat ccgcagttta tccgcaagcg agtgcgggcg ctggcgcata
    11701 aaaggcaccc gcgcttgatc aatacgccgg ctcgagttcc ttggccgact tctggccgct
    11761 ggcgaggcgg ccggccgttt cgcggatgag tttggccgcc tggcggcact gggcttcgct
    11821 gacgtcgagg tgcgtgacca ggcggacctg ctgcgggccg atcgccaggc agagcacgtt
    11881 ctgcgatttc agctcggcgg caaactccgc cgcggatccg agcttgggct cgacgcggaa
    11941 aatcacgatg ttggtctcga ctgcctcggg catcagcgag agtccctcgg cggcgcggat
    12001 cgattcggcc aggatttgcg cattggcgtg gtcctggctc aaccgagcgc ggttgttttg
    12061 cagggcgtac agtgcgccgg ccgcaatgac gccgacttgc cgcatcgcac cgccaaacag
    12121 tttgcggtgc cggcgggcct ccttgatcag gtccttcggg cccgccagcg ccgagccgac
    12181 cggggcgccg agccccttgc tgaagcagac gctcacggtg tcgaagtgct tacaccactc
    12241 cgcctcggga acgccggttg ccgccgcggc gttccacagg cgggcgccgt ccaagtgcgt
    12301 gaccaggcca ctgtcgtgcg cccagcggca gatgccgacg acgttttcca gcggctgaat
    12361 cttgcccgcc ccgcggttgt gcgtgttttc caggcagacc agccgcgtgc ggaccaggtg
    12421 gtcgttttcg ggccgcacca ggccgcggag ctgatcgacg tgcagcacgc cggctttccc
    12481 gtccaccgtg cgggccacga tgccgctgag ctgtgcgaac gcgccctgct cgtagttgta
    12541 gatgtggcag ccggtctcgc agatgaactc gtcgcccggc ttgcagtgaa cccgcacgcc
    12601 gacctggttg gtctgcgacc ccgacgacat gaagatcgcc gcctccttgc ccaggatttc
    12661 ggccgtcagc ttttgcagcc ggtcgcaagt tggatcgacg tcgatcacat cgtcgttcac
    12721 atccgcattg gccatcgcca agcgcatttc cggagacggc cgggtcacgg tgtcggagcg
    12781 gaggtcgatg gacatggtga aatgaattcc agagaaacta gcgcgacgcg gcagccgcag
    12841 gccacggggc agcagcgggc gaggccggcc gataagcgac cttgcgactc gacgacgaaa
    12901 taccgtgcgg cagataaacc tgcaagcgag tttcgttgcc gtccacgatc agcacgattt
    12961 cgcccggcga tgggggaacc tccgcaggat tccgcacaat acaatgctcc ggtccaatct
    13021 ggccgagatc gaactgttgg ccgttcagaa tcaggaacat ctcgacatgt gccgagtagc
    13081 cagcggagtt ggcggtcatg cctgattctc atccggatgt ggaattgcga gcccagcaat
    13141 acgccgacaa agctggcatc actctagtcg aatcgctggg acacggaatt gatggtaacg
    13201 tctggcgtac gagccgccaa tctgcat
//
LOCUS       DASRJB010000475         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2388230, whole genome shotgun sequence.
ACCESSION   DASRJB010000475 DASRJB010000000
VERSION     DASRJB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388230"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..520
                     /locus_tag="VFB80_12510"
     CDS             263..520
                     /locus_tag="VFB80_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN34638.1"
                     /translation="MDQVCDITAKLLSEQRSEITPSTSLGELAADELDIVELVMQLEE
                     HFDISIPDEAVERIMGADNGRKGMNNVTMANLAALVDAQRN"
     gene            complement(554..1321)
                     /gene="rnc"
                     /locus_tag="VFB80_12515"
     CDS             complement(554..1321)
                     /gene="rnc"
                     /locus_tag="VFB80_12515"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZN34639.1"
                     /translation="MSDSVDVAAILPDEVLIEAGPGTDDLLARCEHRIGYRFRERRIL
                     RAALTHASGAEHRLASNERLEFLGDAILGFVVCELLFQQFPQYLEGELTKIKSVVVSR
                     QTCAKISEALGLQEFLILGKGMTTHPSVPPSVLADVFESLIAAIYLDGGVEAARQFIN
                     TYIGPEVELAATGELGGNYKSLLQQFAQREFGSTPTYHLLDEKGPDHSKCFKMVAQVS
                     GQRYQPAWGRNKKESEQRAACNALSELKGEAAPYPSD"
     gene            1554..2549
                     /locus_tag="VFB80_12520"
     CDS             1554..2549
                     /locus_tag="VFB80_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZN34640.1"
                     /translation="MRIVLCYPIEPRHLAEIRAAAAGHEVVDAGQERIAAELLEADIF
                     CGHAKVPVPWDEVVQRRRLKWIQSSAAGLDHCLTPATIASDIIVTSASGLFADQVAEQ
                     TLALLLGILRGLPTFLRAQQRREFIRRPTRDLAGATVGIVGLGGNGTRLAKVLQPFRV
                     RVIATDMFTNQRPPGVAELWPAEHLDRLLAESDVVILCVPLNAQTLGMIAAPQLARMK
                     GGAILINVARGQVVVERDLVAALQSGHLGGAGLDVTEVEPLPAESPLWDLPNVIITPH
                     VGAQSARRSDDTTHLVCDNLRRFFSGRPLLNLVDKQLGFPTPAARTVGLAAHRSH"
     gene            2592..2735
                     /locus_tag="VFB80_12525"
     CDS             2592..2735
                     /locus_tag="VFB80_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34641.1"
                     /translation="MRISLRTLLMLLALEGLLLACAVPIVRELRHAKMRASCVNGLKE
                     QVR"
     gene            2779..>3002
                     /locus_tag="VFB80_12530"
     CDS             2779..>3002
                     /locus_tag="VFB80_12530"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34642.1"
                     /translation="MLSGRFRQGGQISRRAALRAGALGAIGWTLADLLALEARAAPNL
                     AQPRAKSVILLWLWGGPSHLDTFDMKPKAPL"
BASE COUNT          511 a          987 c         1000 g          504 t
ORIGIN      
        1 agggtgcaat tccaatgcct ccattctggc cctggccgca caatccaatc cacgggaatt
       61 ggccaacctc acctttccgc cgcggtaacg taggtttgag caactccagg cgattcttca
      121 cagtcacgaa gcccctcata ccatgccact ccaagcgata ttccgctgcg ttttcctggt
      181 gcttgttggc tgtgccttga gcagcagtgg gtgccaggtt ggcacgccgg ctgctggcgc
      241 cgccgacaac agcagaccaa cggtggacca ggtttgcgac atcacggcga agctgttgtc
      301 cgaacagaga tcggaaatca ctcccagcac ttcgctaggc gagctcgcag ctgatgaatt
      361 ggacatcgtc gagctggtga tgcaattgga agagcacttc gacatttcca taccggacga
      421 ggcggtcgag cggatcatgg gagccgacaa cgggcggaaa gggatgaaca acgtcacgat
      481 ggcaaatctg gctgcactcg tggatgcgca gcgaaactaa cctgcgacac cgtcccgcgc
      541 ggctcagccc gcctcagtcc gacgggtacg gcgccgcttc gcctttgagc tcggaaaggg
      601 cgttgcaggc ggcccgctgc tcggattcct tcttgttgcg gccccaggcg ggctgatagc
      661 gctggcccga aacctgcgcg accatcttga agcatttgct gtggtcgggg cctttttcgt
      721 ctagcaggtg ataggtcggg gtgctgccga attcgcgctg ggcgaattgc tggagcagcg
      781 acttgtaatt gccgcccagc tcgcccgtcg ccgccagctc gacctcgggg ccgatgtagg
      841 tgttgatgaa ctgccgcgcg gcctcgacgc ccccgtcgag atagatcgcc gcgatcagcg
      901 actcgaaaac gtcggccagc acgctcggcg gcacgctggg atgcgtcgtc atccccttgc
      961 cgaggatcaa aaactcctgc aggcccagcg cctcgctgat cttggcgcag gtctgccggc
     1021 tgacgacgac cgacttgatc ttggtcagct ccccttcgag gtactgcggg aactgctgga
     1081 acaacagctc gcagacgacg aatcccagga tcgcgtcgcc gaggaattcc aaccgctcgt
     1141 tcgacgccag ccggtgctcg gcgcccgagg cgtgagtcaa agcggcccgt aggatccgcc
     1201 gttcgcgaaa tcggtagccg atgcggtgct cgcagcgggc gaggaggtcg tccgtgccgg
     1261 gcccggcctc gatcaagacc tcgtccggca ggatcgccgc gacatccacg gaatccgaca
     1321 tattggcccc aaaagcgcca gcccgccgcg agggaacaca gccagcccgc cgcgagggaa
     1381 cacagccggc ccgccgcgag ggaacacagc cctagcctgc tggccctctt gccgcaaccc
     1441 tagctggcat cgaaactttt gtcaacgcaa cggaagggct agggctgggg gctagggcta
     1501 gggaaagcag ctaccccgcg gcccctagcc ctagccccca gcccttagcc ctcatgcgca
     1561 tcgtcctctg ttacccgatc gagccgcggc acctggcgga aatccgcgcg gcagcggcgg
     1621 ggcacgaggt ggtcgatgcg ggtcaggagc ggattgccgc cgagctcctg gaggcggata
     1681 tcttctgcgg gcacgccaag gtgccggtgc cctgggacga agtcgtccag cgccgccggc
     1741 tgaaatggat tcagtcgtcg gccgccgggc tggaccattg cctcacgccg gcgacgatcg
     1801 cgtcggacat tatcgttacc agcgcctcgg ggctgttcgc ggaccaggtg gcggaacaga
     1861 cgctcgcgct gttgctgggc attttgcgcg gcctgccgac cttcttgcgg gcgcagcagc
     1921 ggcgcgaatt catccgccgc ccgacgcgcg acctggccgg cgcgacggtc ggcatcgtcg
     1981 gcctcggcgg caacggcacg cgcctggcga aagtcctgca gcccttccga gtgcgggtca
     2041 tcgccacgga catgttcacg aaccagcggc cgcccggcgt ggccgagttg tggccggctg
     2101 agcacctcga ccgcctgctc gcggaatcgg acgtcgtgat cctctgcgtg ccgctcaatg
     2161 cccagacgct ggggatgatc gccgccccgc agctggcgcg gatgaagggc ggcgcgatcc
     2221 tgatcaatgt ggcccgcggg caggtcgtag tcgagcggga tctcgtcgcg gccctgcaaa
     2281 gcggccacct tggcggggcg gggctcgacg tcacggaggt cgagcccctg ccggccgaga
     2341 gcccgctatg ggacctgccc aacgtgatca tcacgccaca cgtcggcgcg caatccgccc
     2401 ggcggagcga cgacacgacg cacctggtct gcgacaacct gcggcgattt ttcagtggcc
     2461 gcccgctcct caatctggtt gataagcagc tgggctttcc gaccccggca gcgcggaccg
     2521 tggggctggc tgcacaccga agtcactgag taggacgcgg gagtcccaga cctcccctca
     2581 gcggcgaacc gatgcgaatc agcctgcgga cgctactgat gctgctggcc ctcgaagggc
     2641 tgctgctcgc gtgcgcggtt ccgattgtgc gggaattgcg tcacgcgaaa atgcgggcca
     2701 gctgcgtcaa cggcctgaag gagcaggtcc gctaggagcg aatttggtaa ttgcgcgccc
     2761 gtcggctagg ctgatgccat gctgagcggt cgattccggc agggcgggca gatatcgcgg
     2821 cgggcggcgc tccgcgccgg ggcgctgggc gcgattggct ggaccctggc cgatttgctc
     2881 gcgctggagg cccgcgcggc gccgaatctg gcgcagccgc gggcgaagtc ggtgattttg
     2941 ctctggctgt ggggcgggcc gagccacctc gacacgttcg acatgaagcc caaggcgccg
     3001 ct
//
LOCUS       DASRJB010000476         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2097487, whole genome shotgun sequence.
ACCESSION   DASRJB010000476 DASRJB010000000
VERSION     DASRJB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097487"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFB80_12535"
     CDS             complement(<1..252)
                     /locus_tag="VFB80_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34643.1"
                     /translation="MIGLALLILANGVVLGVVAYVLSTWVRLIGLVTAPFWIRLLNSL
                     FDGVLTAQLLLIGLWCALAPERLVQRLLVGIVLAALPVGI"
     gene            complement(386..1933)
                     /locus_tag="VFB80_12540"
     CDS             complement(386..1933)
                     /locus_tag="VFB80_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34644.1"
                     /translation="MTTRRDFLESVGRGMLVAGLGATLTTDLGFSTAFAAEGPAELAF
                     GKYDALVDLLQSTPADKLQPILVEKLTSGETNLKQLMAAGALANAETFGGEDYVGFHT
                     AMAMLPGLNMVGLLPADRQPLPVLKVLYRNSQQIQQYGGASKKTLREMHAAEEATVEN
                     TGVAIRDACRVGDVDKGEKLFSSLEKAPLEDAFNALQPLMQDDVNVHRFVFAHRTYGL
                     ASLLGQDYAYTILRQCVRFSCNHEQGRITRKGQESPIRALMPKLLDQYKLAGWKAGTR
                     DPGDAAVEELAETIYRGPADKSAEAAAAALAEGLSPEVVGEAISLASNLLVLRQGPDP
                     WRTHGDSAGVHSSDATNAFRNMARHSRPEFAVSGLIVAAYHSAAHKAFPGSSYPSTEH
                     RSKIKMTDPEGLLAEAEDAVRNNDQGRAAAAIALYGEGGGSPEAVIQRMLKYTISEDG
                     RLHGEKYFQTVQEEYKTTRPAFRWRQMISLARVTASAYGYYDREDHYGRRAPGYEEAC
                     RLLKVTA"
BASE COUNT          374 a          701 c          697 g          380 t
ORIGIN      
        1 gattcccacc ggcaacgcgg ctagcacaat tcccacgagc aaccgctgaa ccaatcgctc
       61 gggcgctaat gcacaccaaa ggccaatgag caagagctgg gcggttagga ctccgtcaaa
      121 cagactatta agcaggcgaa tccagaaagg ggctgtcaca agtccaatca accgcaccca
      181 ggtggacagt acgtaggcca ccacgcccag caccacaccg ttggcgagga tgagcaaagc
      241 cagcccgatc aagcggcgcc gagtttcagc aaagttgtct gacgagggcg tcacacaccc
      301 aagttggcat gagcggcact gaaaagcaaa aaaccacgcc cactcgctgt gggcgtggtt
      361 ctcttgatag tcgcaggcta acgggctaag ccgtgacctt cagcagccgg caggcttctt
      421 cgtagcccgg ggcgcggcgg ccgtaatggt cttcgcggtc gtagtagccg taagcgctgg
      481 cggtgacgcg ggccaggctg atcatctgcc gccagcggaa ggcgggccgc gtcgtcttgt
      541 actcctcctg cacggtctgg aaatacttct cgccgtgcag gcggccgtcc tcgctgatcg
      601 tgtacttcag catccgctgg atcaccgcct cgggcgagcc gccgccttcg ccgtagaggg
      661 cgatcgcagc cgcggcccgg ccctggtcgt tgttgcggac cgcgtcttcc gcctcggcca
      721 gcaagccctc gggatcggtc atcttgatct tcgagcgatg ctcggtcgag ggatacgacg
      781 agccggggaa cgccttgtgg gccgccgagt ggtaggcggc gacgatcagg ccagacaccg
      841 caaactcagg ccgcgagtgg cgggccatgt tgcggaacgc gttggttgcg tcggaggagt
      901 gcacgccggc cgaatcgccg tgcgtacgcc acggatcggg cccttgccgc aggaccagca
      961 ggttcgacgc cagcgagatc gcctcgccga cgacttcggg gctgagccct tcggccagtg
     1021 cggcagccgc ggcctcggcc gacttgtccg ccggaccacg ataaatcgtc tcggccaatt
     1081 cctcgaccgc ggcgtcgccc gggtcgcgcg tgccggcctt ccagccggcc agcttgtact
     1141 ggtcgagaag cttgggcatc agggcgcgaa tcggcgactc ctggcccttg cgggtgatgc
     1201 ggccctgttc gtggttgcag gagaaccgca cgcactgccg caggatcgta taggcgtagt
     1261 cctggcccag caggctggcc agcccatacg tccgatgggc gaagacgaag cggtgcacgt
     1321 tgacatcgtc ctgcatgagg ggctgcagcg cgttgaaggc gtcctccagc ggcgccttct
     1381 ccaggctgga aaacagcttc tcgcctttgt ccacgtcgcc gacgcggcag gcgtcgcgga
     1441 tggccacgcc cgtgttttcg acggtcgcct cttcggcggc gtgcatctcg cggagcgtct
     1501 tcttcgaagc gccgccgtac tgctgaatct gctgcgagtt gcgatagagc actttgagca
     1561 ccgggagcgg ctggcgatcg gccggcagca gccccaccat gttcaatccc ggcagcatcg
     1621 ccatcgcggt gtggaagccg acgtagtcct cgccgccgaa cgtttcggcg ttggccagcg
     1681 ccccggcggc catcagctgc ttcaggttcg tctcgccgct ggtgagcttc tcgaccagga
     1741 tcggctgcag cttgtcggcg ggcgtgctct gcagcagatc gaccagtgcg tcgtacttgc
     1801 cgaacgccag ctccgccggc ccttcggccg cgaaggcggt ggagaagccc aggtcagtgg
     1861 tcagcgtcgc gccgagtccg gcaacgagca ttccgcgtcc aaccgattcc agaaaatcgc
     1921 gacgggtggt catgggcttg ctccgagtgt gtggggagag gcgtttcctt agataacgag
     1981 agcgcgttac acctgaaaag ctgccggacg taacgccttg ccgttaatat agttgtcgcc
     2041 gcctactcaa tgccatagct ggctccgccc ggcggctttc cgacccatgt ctccctgccc
     2101 cgacagcccg actttgcagg cctttgccat cggtaagctc ggaggcagcg ag
//
LOCUS       DASRJB010000477         7895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2953680, whole genome shotgun sequence.
ACCESSION   DASRJB010000477 DASRJB010000000
VERSION     DASRJB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953680"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..928
                     /locus_tag="VFB80_12545"
     CDS             92..928
                     /locus_tag="VFB80_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34645.1"
                     /translation="MPRLLTIAGFLVLTAPALAAEPGLTMSWEKNYLTIRGDFPGQEL
                     KILYLEAYCRPGSTDRDWKETVIPHTAEQLEASEGGRTIKLRDKLADGVVVEHSLTAS
                     TDEIDFRLVAHNPTDKASEAHWAQPCIRVDRFTGTTNTDARTLVPAYARKCFLFLDGK
                     LTRMPTYPWADKARYEPGQVYCPQGVDRNDINPRPLSKLVPSSGLCGCFSADEKQIMA
                     VAWEPYQEIFQGVIACLHSDFRVGGLASGETKKIRGKLYIVPADVEKLVARYERDFPE
                     QK"
     gene            complement(1116..2972)
                     /locus_tag="VFB80_12550"
     CDS             complement(1116..2972)
                     /locus_tag="VFB80_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN34646.1"
                     /translation="MSLVDLLGHPAARLLTLALAHFLWQGALIAVLLAAAVQLLGVRR
                     ATSRYACSLAALAAMLICPLVTLGWLSLPGEEPVGWAVPTSGVLMVGTAHPTLSELLA
                     PLQPVALAAWLCGVALFGTRLLAGAIGVARLRRHKLPLPLDLASLVERLGRRLKLDAL
                     PLVFVSRQATEAMAVGLLRPLVLIPAAWVTEMPLDMLEAVIAHELAHLRRRDLWVNLL
                     QRLAETLLFYHPAIWWLSRRLRTERELCADELAVAVTGRRLEYAQALEQVAHRRQAEI
                     RPALAAFMRGETNMRVLERVRNVLGLAGGERSRLWPVGIVALALPAVLWIAAAGWRSA
                     VADEPKDGDKPAVKRDGDRPDGDKEPVVRKIEARFFAKQDRDEDGEVKKDRPRDGEVK
                     KDAPRDGEVKKDATRRDGEVKKDAPRDGEVKKDATRRDGEVKKDAPRDGDVRNLRKVV
                     AREGDAASIEELTALVKRLSAQVEKLQAEVSALRGGRGEANEKVPVTRRIKEGAGDKE
                     ALKEAIKREAATDKDAIKREAAEAIRARELKTREGGDKPTGENARRAAEAKEREAVGR
                     RFKDAILKEVENKEAAVKERELIERKKALAAEIEKKAAAEKEAAAKKEKEDK"
     gene            complement(2974..3366)
                     /locus_tag="VFB80_12555"
     CDS             complement(2974..3366)
                     /locus_tag="VFB80_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN34647.1"
                     /translation="MARPKEEQPTAGELEVLKVLWDRGASTVREVMEVLNAERPRAYT
                     SVMSLLNVMTDKGLLVREPQGRAFVYRPKTDRGRTLKRMVGDLLGRAFEGSTSQLVAH
                     LLDQSKPTAAELAEIRRAIEQYQSLKGG"
     gene            complement(3471..4526)
                     /locus_tag="VFB80_12560"
     CDS             complement(3471..4526)
                     /locus_tag="VFB80_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34648.1"
                     /translation="MTKTATTQTKQTRSRRPWTPDALHLEIFERVRMLGEKQQEIALS
                     LGINQSTVSRIVARVERWQSRATPKEAGRLTHGERLRTQTSLTFERNELLLASCLRMA
                     SRMESTTNYVHTTTRRQDPATREKITLCDEQLDSCGVAARFLRLAFRINMEQLKLVRE
                     EELPPLPPLSAEELHEEELGADAAAAELAASLQQYREQFDENHRQDMEHLKEQERLKA
                     QEEAERAELERQLAETGVGGQESGVGEEPVAGTLRVPSPADDHPPASDGSRSEPATHN
                     LHKVHKANGHASAASAGSEETYDENRGAEKSANDACMKPRKRNPSVRNVWSEAEPGTK
                     ERRSARRPPAPSPSPQP"
     gene            4882..5139
                     /locus_tag="VFB80_12565"
     CDS             4882..5139
                     /locus_tag="VFB80_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34649.1"
                     /translation="MQPVATNRNVELPGTWPIRGPYEILAERAGGVGPTTAAFRDRGH
                     LDRVLERIPRRDCVTRTAYPDERAIRVDVQRLRERALAAAR"
     gene            complement(5210..6331)
                     /locus_tag="VFB80_12570"
     CDS             complement(5210..6331)
                     /locus_tag="VFB80_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN34650.1"
                     /translation="MKTFWYLAAVVLLAGAAVAAAIWSRSRSADSYQPRLESDPPPPS
                     PPQWAVSDFNAFLERLRLAAGRRESLLLHRDHLERAEAAFKSTAGVPADVQLEMHAPF
                     FAVDPEAIYALYADLPTLERGLALFQQNCSGCHGSFGRGNGAATREWYVGNYPRNFWY
                     GKYKSRSTPYAQLPEDGDLFRTLTRGMYGSSMPSFRSLSEADRWCLVQFLKTLANFDD
                     EYENKVVNRFDPAEDKSQPPPAIGPEPPVSLESVTRGRILFIEQACVSCHQGNKPKPV
                     GLGRWEGNFNWYDEMQRPVAHSRDLTTGVFRSGAASSDVFRIITFGPNVGPMPSYQNI
                     PEADRWALVHYVRSTYKSDYPQAPASADAQAKKPAPLPQ"
     gene            complement(6454..7233)
                     /locus_tag="VFB80_12575"
     CDS             complement(6454..7233)
                     /locus_tag="VFB80_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34651.1"
                     /translation="MRTFFLLAAIFALGGVCTLAVTSFMNGRGAVAQESVITPRSQPG
                     TDMTIYSPKLHGLYDGKFKLSCGRTYVVGSLSDKEGWEHMDNAGTNSKLTEGTVDIDV
                     DEIKNTGKFVAKLKLPEGMFEMTLDNFKESLACQDGGVASMIYEHGDSGCGNTLWPKT
                     ILYIAAWGKASATLNGKPLYKDYDAHFMVTQGIRHRETLKVDYPQVSRKDIFGKPSLA
                     GEVNPAAMQCDFWIRSPQQNNKNNPPHEHFMHFYSLEVTWK"
     gene            complement(7250..>7895)
                     /locus_tag="VFB80_12580"
     CDS             complement(7250..>7895)
                     /locus_tag="VFB80_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZN34652.1"
                     /translation="EIPAQTMVPIATDPEICQKHGMDGLYPMEDYVIDRQTRGIRYVI
                     VYLQSDAIRKWAGTPRQDATIDNRNCRFEPHVALATVGSQVEVKNSDDVYHTTHLYGP
                     AGFQYNPGLRAKGQSERTELTRPGTYIVKCDRHGWMSAFLRVDDHPFHAVTDEKGRFE
                     IKGIPPGKHRVEVFHEKFGERPIAQPAGGNTAPEVIEVNIEADKTSTLDLKLSH"
BASE COUNT         1223 a         2715 c         2452 g         1505 t
ORIGIN      
        1 acggtctggg ggttgattct cgcggccgca gacagtgtga tgacgcgtgc cgagagggat
       61 tagttcgcag tctaagacgg gagaaagcca gatgccacgg ctgctgacga ttgcgggttt
      121 cctcgtactg acggcgccgg ccctggctgc cgagcccggg ctgacgatgt cctgggagaa
      181 gaactatctc accatccgcg gcgactttcc cggccaggag ctgaagatct tgtacctgga
      241 ggcctattgc cggccgggct cgaccgaccg cgactggaag gagacggtca tcccgcacac
      301 cgccgagcag ctcgaggcca gcgagggcgg ccgcacgatt aagctccgcg ataagctggc
      361 cgatggcgtg gtcgtggagc attcgctcac cgcgagcacc gatgagatcg acttccggct
      421 cgtcgcccac aatccgaccg acaaagcctc cgaggcccat tgggctcagc cctgcattcg
      481 cgtcgatcgc ttcaccggca cgaccaacac cgacgcccgc acgctcgtgc cggcctacgc
      541 ccgcaagtgc tttttgttcc tggacggcaa gctgacgcga atgcccacct atccctgggc
      601 cgacaaggcc cgctacgagc ccggccaggt ctattgcccc caaggcgtcg atcgcaacga
      661 catcaacccg cggccgctca gcaagctcgt gccgtcgagc ggcctgtgcg gctgcttttc
      721 ggccgacgag aagcagatca tggccgtcgc ctgggaaccg taccaagaga tcttccaggg
      781 cgtgatcgcc tgcttgcaca gcgactttcg cgtcggcggc ctcgcctccg gcgagacgaa
      841 gaaaatccgc ggcaagctct atatcgtgcc cgccgacgtc gagaagctcg tggcgcggta
      901 cgagcgggac tttccggaac agaagtaggc ccggagggcc ggcaggtaat agccccgacc
      961 gtcaggtcgg ggattgttga atcacgaaat cgcagaggcc cggagggccg gcactttgcc
     1021 ggccctccgg gccttatcat tcaatcgcgt ttcgaaaacc ccgacctcac ggtcggggct
     1081 actgcctgcc ggccctccgg gcctgaggca ggctgctact tgtcctcttt ctccttcttg
     1141 gcggcggctt ccttctccgc ggcggccttc ttctcgattt ccgcggccag ggccttcttc
     1201 cgctcgatca gctcccgctc cttgacggcg gcctctttat tctcgacttc cttcaggatc
     1261 gcgtccttga agcggcggcc aacggcttct cgttccttgg cctcggcggc gcggcgagcg
     1321 ttctcgccgg tgggcttgtc gccgccttcg cgggtcttca attcgcgagc gcgaatcgcc
     1381 tcggcggctt cccgcttgat ggcgtccttg tcggtggcgg cttcccgctt gatcgcctcc
     1441 ttgagggctt ccttgtcacc ggcgccttcc ttgatgcggc gggtcacggg taccttctcg
     1501 ttggcttcgc cccggccgcc gcggagggcg ctcacttcgg cttgcagctt ctcgacctgc
     1561 gccgacaggc gcttcacgag cgcggtcagc tcttcgatgc tggcggcgtc gccttcgcgg
     1621 gccacaacct tgcgtaggtt tcgcacgtcg ccgtcccgcg gcgcatcctt cttcacttcg
     1681 ccgtcgcgcc gtgtggcgtc cttctttacc tcgccgtccc gcggcgcatc cttcttcact
     1741 tcgccgtcgc gccgcgtggc atccttcttc acctcgccat cccgcggcgc gtccttcttc
     1801 acttcaccgt cccgcgggcg atcctttttc acttccccgt cttcgtcgcg gtcttgcttg
     1861 gcgaagaacc tggcttcgat cttgcgtacg acgggctcct tgtcgccgtc cgggcgatcg
     1921 ccgtcgcgct tcacggcagg cttgtcgccg tccttcggct cgtcggccac cgccgaccgc
     1981 cagccggccg ccgcgatcca caacaccgcc ggcagcgcca acgccacgat tcccaccggc
     2041 cacagccgcg accgctcgcc gccggccagg cccaacacgt tccgcacccg ttccaggact
     2101 cgcatgttcg tttctccccg catgaacgcc gccagcgcgg gccggatttc ggcctgccgc
     2161 cggtgggcga cttgttccaa agcctgggca tattccagcc gccgccccgt gacggcgacc
     2221 gccaattcat ccgcgcacag ctcgcgctca gttcgcagcc gccgcgacag ccaccagatg
     2281 gccggatgat aaaagagcaa ggtctcggcc agccgctgca gcaggttgac ccacaggtcg
     2341 cgccgccgca aatgggccaa ctcgtgggca attaccgcct ccagcatgtc cagcggcatc
     2401 tccgtcaccc acgccgccgg gatcagcacc agcggcctca agaggcccac cgccatcgcc
     2461 tcggtggcct gccggctgac aaacaccagc ggcaaggcgt cgagctttag gcggcggccc
     2521 aatcgctcga cgagcgaagc caggtccagc ggcagcggca gcttatggcg gcggagccgg
     2581 gccacgccga tcgcgccggc caggagccgc gtgccgaaca gcgccacgcc gcacagccag
     2641 gccgccaagg ccaccggttg cagcggggcg agcagttcgg agagcgtagg gtgggctgtg
     2701 cccaccatta atactccaga ggtgggcaca gcccacccta cgggctcctc gccaggcagc
     2761 gacagccagc cgagcgtgac cagtgggcag atcagcatcg ccgccagcgc cgccagcgaa
     2821 caggcatagc gcgatgtcgc gcggcggacg cccagcagct gcacggccgc ggcgagcagc
     2881 acggcgatga gcgctccctg ccacaggaaa tgcgccaagg ccagcgtcag caggcgcgcg
     2941 gccggatgcc ccagcagatc gacgagcgac atattaaccg cccttcagtg actggtattg
     3001 ttcgatggct cggcgaatct cggccagctc ggccgccgtc ggcttgctct ggtcgagcag
     3061 gtgggcgacc agctggctcg tggagccttc gaaggcccgc cccagcagat cgccgaccat
     3121 ccgcttcagc gtccggccgc ggtcggtctt cggccgatac acaaacgccc gcccctgcgg
     3181 ctcgcggacc agcagcccct tgtcggtcat cacgttgagc aggctcatca ccgacgtgta
     3241 ggcccgcggg cgctccgcgt tgagcacttc catcacttcg cggacggtgc tcgcgccgcg
     3301 gtcccaaagt actttgagga cttccagctc accagccgtc ggctgctctt ccttcggtcg
     3361 ggccatgtca gacaagctcg cgagtacaaa cgaacgaaaa agaccaacgg actcattcta
     3421 cggtttatgc cgtagaagtc aacgggttaa accgttgata gattctaggg ctagggctgg
     3481 ggactagggc taggggctgg tggtcggcga gcggagcgcc tctccttggt accaggctct
     3541 gcttcggacc acacgttccg gacgctcgga ttccgcttcc gcggcttcat gcaggcatca
     3601 ttggcgcttt tttctgcgcc gcgattctcg tcgtaagtct cttcactgcc ggcacttgca
     3661 gccgatgcat ggccgttggc tttatgcacc ttatgcaggt tatgcgtagc aggctcactc
     3721 cgtgagccgt cacttgcggg tggatgatcg tcggcgggcg acggcacacg gagtgtgcct
     3781 gctactggct cctctccgac gcccgactcc tgacccccga ctcccgtctc cgccagctgc
     3841 cgctccagct cggcccgctc ggcctcctcc tgggccttca gccgctcctg ctccttcaaa
     3901 tgctccatgt cctggcggtg gttctcgtcg aactgctcgc ggtactgctg cagcgaggcc
     3961 gcgagctcgg cggctgcggc gtcggcgccg agctcttcct cgtgcagctc ctcggccgag
     4021 aggggcggca gcggcggcag ctcctcctcg cggaccagct tcagctgctc catgttgatc
     4081 cggaacgcca gccgcaaaaa ccgcgcggcg acgccgcagc tgtcgagctg ctcgtcgcag
     4141 agcgtgatct tctcgcgcgt ggccgggtcc tgacgccggg tcgtcgtgtg cacgtagttg
     4201 gtcgtcgatt ccatccgcga ggccatgcgg aggcagctgg ccaagagcag ctcgttccgc
     4261 tcgaaggtca gcgaggtctg cgtccgcagc cgctcgccgt gcgtcaggcg gccggcttct
     4321 ttgggcgtgg ctcgcgactg ccagcgttcg acgcgggcca cgatccgcga gacggtcgat
     4381 tggttgatcc ccaggctgag ggcgatctcc tgctgcttct cgcccagcat gcggacccgc
     4441 tcgaagatct ccaggtgcaa ggcatccggc gtccacggcc ggcggctgcg ggtctgtttg
     4501 gtctgggtgg tcgcggtctt cgtcatggca agtctcctga gtaggttccg cctgccgggc
     4561 ggaacctgat cgctggggaa cgtcttttct ggccgcagag gtctcgcccg gcaggcgaga
     4621 cctactaaat gcgctttctg tacacgtatg ctcaggttct gctacttgcg tgaacacccg
     4681 tcaacaaccc cccgcccaga attctcaccc ccctgcctgc acgcctcgcc gcaacctctt
     4741 tcccccacga cctttacgac cagagaaatt tcgcgaaatt ctcgccgacc ccgtttcaga
     4801 atggactagt gtggtccatt ccagacggcc ctctttcggg gtgctcggac agatttccga
     4861 ggccccgagg gcctgctttc gatgcaacca gtggccacca accgcaacgt agagttgcct
     4921 ggcacctggc ccatccgcgg tccctatgag atcctggccg agcgcgccgg gggcgtcggt
     4981 ccaacgaccg cggcctttcg cgaccgaggt cacctcgacc gcgttttgga gcgaatcccc
     5041 cgccgcgatt gcgtcactcg caccgcctat cccgacgaac gcgcaatccg tgtggacgtc
     5101 cagcggctcc gcgaacgagc gctggccgcg gcgcgctaac gtcgagcact cgaaccgcgg
     5161 tgggcacagc ccaccctact tttcttgcgc aaatgccctt cggcgggtct cattgcggca
     5221 ggggcgctgg cttcttggcc tgagcgtcgg cggaggcggg ggcctgcgga tagtcgctct
     5281 tgtaggtcga gcggacgtag tgcaccagcg cccagcgatc ggcctcagga atattctgat
     5341 agctgggcat gggtccaaca ttggggccga aggtaatgat ccggaaaacg tcgctgctgg
     5401 ctgcgccgga gcggaacacg cccgtcgtca aatcccgcga gtgtgcgacg gggcgctgca
     5461 tctcgtcgta ccagttgaag ttcccctccc agcggcccag gcccaccggc ttcggcttgt
     5521 tcccctggtg gcagcttacg caggcttgct cgatgaacag aatccgcccg cgggtcaccg
     5581 attccaggct caccggcggc tccggcccga tcgccggcgg cggttgcgac ttgtcctctg
     5641 cggggtcgaa gcgattgacc actttgttct catactcgtc gtcgaagttg gccagcgttt
     5701 tcaggaactg aaccaggcac cagcgatcgg cctcgctcag gctgcggaac gacggcatcg
     5761 acgagccgta catcccgcgg gtcagcgtgc ggaacaggtc gccatcctcg ggcaattggg
     5821 catagggcgt cgagcggctc ttgtatttcc cgtaccagaa gttgcgcgga tagtttccca
     5881 cataccactc gcgcgtcgcc gcgccgttgc cgcgcccaaa gcttccgtgg cagccgctgc
     5941 agttctgctg gaacagggcc aggccgcgct ccagcgtagg caggtcagca tagagggcgt
     6001 agatcgcctc gggatcgacc gcgaagaacg gggcgtgcat ttccagttgc acgtcggccg
     6061 gcactcccgc cgtgcttttg aacgcggcct cggcgcgctc cagatggtcg cgatgcaata
     6121 gcagcgactc ccgccgccca gcggccagcc gcagccgctc caggaaggcg ttgaagtcgc
     6181 tgaccgccca ctggggcggc gagggcggcg gcggatccga ctccagccgc ggctgatacg
     6241 agtccgctga gcgcgaccgc gaccagatcg ccgccgccac agctgcgcca gccaggagca
     6301 ccacggcggc cagataccag aaggtcttca tggtttggcc acattcaacc gtttaacccg
     6361 gagccggagg cgacggcgaa ctcgcgaaat gcctgcgcct ccggctacgg ctacggctac
     6421 gggttaaacg agttagcggc cgcgggcggc gttttacttc cacgtcactt ccaacgaata
     6481 gaagtgcatg aagtgctcgt gcggaggatt gttcttgttg ttttgctgcg ggctgcgaat
     6541 ccagaagtcg cactgcatgg ccgcggggtt gacctcgccg gccagcgacg gcttgccgaa
     6601 aatgtccttc cgcgaaacct gcggataatc gaccttcagc gtctcgcggt ggcggatgcc
     6661 ctgcgtgacc atgaagtggg cgtcgtagtc cttgtacagc ggcttgccgt tcaaagtggc
     6721 gctggccttg ccccaggcgg cgatgtagag gatcgtcttg ggccacagcg tgttgccgca
     6781 gcccgagtcg ccgtgctcgt agatcatcga ggcgacgccg ccgtcctggc aggccaggct
     6841 ctccttgaag ttgtcgagcg tcatctcgaa cattccctcc ggcagcttca gcttggccac
     6901 gaacttgccg gtgttcttga tctcgtccac gtcaatatcg accgtgccct cggtgagctt
     6961 ggagttggtg ccggcgttgt ccatgtgctc ccagccctct ttgtcggaca ggctgcccac
     7021 cacataggtc cggccgcagc tcagcttgaa cttcccgtcg tagaggccgt gcagcttggg
     7081 gctgtaaatc gtcatgtcgg tgccgggctg gctccgcggc gtgatcacgc tttcctgggc
     7141 gaccgccccc cggccgttca tgaaggaagt cacggccagg gtgcacactc ctccgagcgc
     7201 aaatatggcc gccagcaaga agaaggtccg catcgccgtc gctccttttc taatgggaaa
     7261 gcttgagatc cagcgtagag gtcttgtcgg cctcgatatt gacttcgatc acttcgggtg
     7321 ctgtatttcc accggcgggc tgcgcgatgg gccgctctcc gaatttctcg tggaacacct
     7381 cgacgcggtg tttgccgggc ggaatgcctt tgatctcgaa gcggcctttc tcgtcggtga
     7441 ccgcgtggaa cggatggtcg tccactcgca ggaacgcgct catccacccg tgccgatcgc
     7501 acttgacgat gtacgtgccg ggccgcgtca gctccgtccg ctccgactgg cccttggctc
     7561 gcagccccgg gttgtattga aacccggccg gcccatacag gtgcgtcgtg tgatacacgt
     7621 cgtcgctgtt cttcacctcg acctggctgc ccacggttgc caaggccacg tgcggctcaa
     7681 accggcagtt gcggttgtcg atggtcgcgt cttggcgcgg agtgcccgcc catttgcgga
     7741 tcgcgtcgct ctggaggtag acgatcacgt agcggatgcc gcgcgtctgg cggtcgatca
     7801 cgtagtcctc catcggatac agcccgtcca tcccgtgctt ctggcaaatc tccgggtccg
     7861 tggcgatggg caccatcgtc tgcgccggga tttcg
//
LOCUS       DASRJB010000478         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1258324, whole genome shotgun sequence.
ACCESSION   DASRJB010000478 DASRJB010000000
VERSION     DASRJB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258324"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFB80_12585"
     CDS             complement(<1..908)
                     /locus_tag="VFB80_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34653.1"
                     /translation="MTRQIFGWLVCTLLAAGWVAAQEPAAPAAAPVPAAEAGPTSADE
                     LALNQSQIADKYARLEQLMLKMAELEGVTNPKRAQLLTRAVEQSKERLTKTQLETLVT
                     LLNQKQLKRAIDGQATVQTDLKALLDLLMSEDRSDRLKSEQQRIKEYIKEVERLIRLE
                     KSVQGQNEGGADPKKVAQEQGRIAGQTGDLSRKIRENEEGGAPQQPGEQKPGENDGQQ
                     GKPGEQKPGEQKPGEQKPGEQKPGEQKPGEQKPGEQKPGEGQQGQQGQQGQQGQQGQQ
                     GQQGQQGQQGQQGQQGQQQQQQKQQA"
     gene            complement(975..3410)
                     /locus_tag="VFB80_12590"
     CDS             complement(975..3410)
                     /locus_tag="VFB80_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34654.1"
                     /translation="MATAIPKLALTPDLRSLLAGLRWRIRLYIWLEGLALAVIWVGLM
                     FWVGLALDYLPVLLGASEMPAVARGVLLAATGVMLAYILYRWIGRRTFVRLSDRSMAL
                     LLERRYSGFHDSLVTAVEMSGAPDHASAFSRDLLGLTTKEARAEVGHVRYRQVFNNGS
                     LAWKVLLAAVVAGSVALFFSTNAAAAGRATERLLLLSNEPWPRSAKIEVTGIEVLREA
                     APGEDSPRTITLPFVNRMVKVAKGANVSLKVRAAQAPEAQVVPQHCTIYYRTRTSGEG
                     IRGERGSVTMSNFRDVISAGGSGSSRNFWFDGKPFKGVLSTVEFDVVGFDHRVGGYKL
                     EVVDSPAVVETLLDLEFPKYMVDEAISSRLPASDQPYLPAGTFIPTGTRVTLKFRSNK
                     ELLQAEIAVEGTDQRTVIDISPTAADRFRFSHRIDALPGSQTLSISLIDHDNVTTERP
                     FRVFLTAIEDQPPQVEVSLKGIGSSVTPDVTVPFRGKVSDDYAVAKSWFEVRVNESGD
                     PHDVAVAIGKAGAVDQEIDFRRARTDKTGLEIKPGDKLFLAVKAADKFDLPDSSGQPG
                     QPHEASGERYQLDVVTPEQLLAQLEVREVGLRRRFEQIIDELTQMRDSLLRVKASVSP
                     GAAAADPEDLRSDDDPEGKQLSPEQKAQRAADFRLLRVQRAIQQSQKSVAEVAGVAAG
                     FLDIREELINNRVDTEDRKKRLKDLIADPLNTTCAEEFPKLDQQMQVLETKLREAGVK
                     LVPAEASPLADSAIDQANAVLAKLEEVLARMQDLETYNELLEIVRDLLKDQQKLSERT
                     DQERKRQIRDELK"
     gene            complement(3478..>5097)
                     /locus_tag="VFB80_12595"
     CDS             complement(3478..>5097)
                     /locus_tag="VFB80_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN34655.1"
                     /translation="QAQNYSRPSVELKLYEAEADGSNESVADSKTVDVGRLGQVATVK
                     FERKPDVQGVRRYRLAAATFTGEIDTKDNEKTAKVEIIDRRTRVLLIAGGPMRDFLFL
                     RNQLFRDKEVISDVWLQSGKPGISQEANEVLYKFPDSADELFEYDAIVAFDADWEQLD
                     EDHVRLLERWVAEKAGGLVVVAGPVFTPQWSSRRRGDPRIDLIKALYPVAFYYQGAAT
                     LSLGRFGSDKTWPLTFTRDGLEAEFLWLDENALASEKAWSQFEGVSGYYAVKDFKPGA
                     RVYSRFSDPDTAIDNVQPIYMAGQFYGSGRVFFMASGEMWRVRAVDDVYFEQFYTKLI
                     RWVSEGRLLRDSSRGVLLVDKDRATLGDQVAVRAILQDAQHQPLTMDEVPAVIVKPDT
                     TRAALTMKKVKDEGREGIYVEQFTALQEGDYQIQLQHPAAAEQVLTREVRVKIPAKES
                     ESPERNDALLREIADKSGGDYFVGLASAVGRDAGGRPSILNLIKPQDQVTPLPGTPDR
                     SFEQQLMTWLLGVICGALCLEWLLRRLSKLA"
BASE COUNT          759 a         1730 c         1593 g         1015 t
ORIGIN      
        1 tgggcctgct gcttctgttg ctgttgctgc tggccctgtt gcccttgttg tccctgctgt
       61 ccttgttgtc cctgctgtcc ttgttgtccc tgctggccct gttgtccctg ctgtccttgc
      121 tgaccctgtt ggccctcgcc cggcttctgc tcaccgggct tctgctcgcc gggtttctgt
      181 tctcctggct tctgttctcc tggcttctgc tcaccgggtt tttgctcacc gggtttttgc
      241 tcgcccggtt tgccctgctg gccgtcgttt tcgccgggct tttgctcgcc gggctgctgg
      301 ggagcaccgc cctcttcgtt ttcgcgaatc ttccgcgaca ggtcgcccgt ctggccggcg
      361 atgcggccct gctcctgggc caccttcttg ggatcggcgc cgccttcgtt ctggccctgc
      421 accgattttt ccaggcggat cagccgctcg acctctttga tgtactcctt gatccgctgc
      481 tgttcgctct tgaggcggtc cgagcggtcc tcgctcatca gcaggtccag gagcgccttg
      541 agatcggtct ggacggtggc ctggccgtcg atggcccgct tgagctgctt ttggttgagc
      601 agcgtcacca gcgtctcgag ctgcgtcttg gtcagccgct ccttcgactg ttcgacggcc
      661 cgcgtcaaaa gctgggcccg cttgggattg gtgacgccct ccagctcggc catcttgagc
      721 atcagctgct ccaagcgggc gtacttatcg gcgatctggc tctggttgag ggccagctcg
      781 tcggccgagg tcggccccgc ctcggcagcc gggaccggcg ccgcggcagg cgccgctggc
      841 tcttgcgcgg caacccatcc cgctgccagc aaggtgcaga ccagccatcc gaatatctgt
      901 cttgtcatcg ctgactcctt caggttcaga cgattccgat cctcactttg attcgacggg
      961 aacttgcttc ctgcctactt gagttcatcc cgaatctgcc gcttgcgctc ctggtcggtg
     1021 cgctcgctga gcttctgttg atctttaagc aaatcccgga cgatctcaag caattcgttg
     1081 taagtttcca ggtcctgcat gcgggccagg acttcctcca gcttcgccag gacggcgttg
     1141 gcctggtcga tcgcgctgtc ggccagcggc gacgcttcgg ccggcacgag cttcacgccg
     1201 gcctcgcgga gcttcgtctc caggacctgc atctgctggt cgagcttcgg aaactcctcg
     1261 gcgcaggtgg tgttcagcgg gtcggcgatc aggtccttca gccgcttctt gcggtcttcg
     1321 gtatcgacgc ggttgttgat cagctcttcg cggatatcga ggaagccggc agcgacgccc
     1381 gcaacctcgg ccacggactt ctggctctgc tggatcgccc gctgcacgcg gagcaagcgg
     1441 aaatcggcgg cccgctgggc cttctgctct gggctaagct gcttgccttc ggggtcgtcg
     1501 tcgctgcgga ggtcttccgg gtcggcggcc gccgcgccgg gcgagacgct ggccttgacc
     1561 cgcagcagcg agtcccgcat ctgcgtcagc tcgtcgatga tctgctcgaa ccgccgccgc
     1621 aggccgacct cgcggacctc cagctgcgcc agcaactgct cgggcgtaac cacgtcgagc
     1681 tggtagcgct cgccgctcgc ctcgtgcggc tggccgggct ggcccgagct atcgggcagg
     1741 tcgaatttgt cggctgcctt gacggccagg aagagcttgt cgccgggctt gatctccagg
     1801 ccggtcttgt cggtccgcgc gcggcggaaa tcgatctctt gatcgaccgc gccggccttg
     1861 ccgattgcca ccgcgacgtc gtgcggatcg ccgctctcgt tgacccgcac ttcgaaccag
     1921 ctcttcgcga cggcataatc gtcgctgacc ttgccgcgga agggcacggt cacgtcgggc
     1981 gtcaccgagc tgccgatgcc tttcagggag acctcgacct gcggtggctg gtcctcgatc
     2041 gcggtgagaa acactcgaaa cggccgctcg gtcgtgacgt tgtcgtggtc gatcagcgag
     2101 atgctcaggg tctgcgagcc cggcagggcg tcgattctgt ggctgaagcg aaagcggtcg
     2161 gccgcggtcg gcgaaatgtc gatcaccgtc cgctggtctg tgccctcgac ggcgatctcg
     2221 gcctgaagca gctccttgtt gctcctgaac ttcagcgtca cccgcgtccc cgtggggatg
     2281 aatgtgccgg caggcaagta cggctgatcg ctggccggca ggcggctcga aatcgcctca
     2341 tcgaccatgt acttcggaaa ttccagatcg agcagcgtct cgacgaccgc cggactgtct
     2401 acgacctcga gcttgtagcc gccgacgcgg tgatcgaagc cgacgacgtc gaactcgacg
     2461 gtcgagagca cgcccttgaa cggcttgccg tcgaaccaga agttgcgcga gctgccagag
     2521 ccaccggccg agattacgtc gcggaagttg ctcatcgtga cgctgccccg ctcgccgcgg
     2581 atgccttcgc cgctggtgcg ggtgcggtaa tagatggtgc agtgctgcgg cacgacctgg
     2641 gcctcggggg cctgggcggc gcggaccttg agcgaaacgt tcgccccctt ggcgaccttc
     2701 accatgcggt tcacgaaggg cagcgtgatc gtccgcggcg aatcctcgcc cggcgcggct
     2761 tcgcggagca cctcgatgcc cgtcacttca atctttgcgc tccgcggcca gggttcgttg
     2821 ctgaggagca gcagccgctc ggtcgcccgt cccgcggccg cagcgttcgt gctgaagaac
     2881 aaggccaccg accccgccac gacagcagcc aacagaactt tccaggcgag cgaaccgttg
     2941 ttgaagacct gccggtagcg gacgtggccc acctccgcgc gggcttcctt cgtggtcaga
     3001 cccaagagat cgcggctgaa cgccgaggcg tggtcgggcg cgccggacat ctcgaccgcc
     3061 gtcaccaggc tatcgtggaa gccggaatag cgccgctcca ggagcagcgc catgctgcgg
     3121 tcactgaggc ggacgaacgt gcggcggcca atccagcgat agaggatgta ggccagcatt
     3181 acgcccgtcg ccgcgagcaa gacgccgcgg gccacggccg gcatctcact ggcccccagc
     3241 agcaccggca gatagtcgag cgccaggccc acccagaaca tcaggccgac ccagatcacc
     3301 gccagcgcga gcccctccag ccagatatag aggcgaatcc gccaccgcag acccgcgagc
     3361 agcgatcgca ggtcgggcgt caaggcgagt ttgggaatcg cggtggccat ggacgtcgta
     3421 attctatcag cgttcgttaa tttgagtaaa actcaatatt ttgtagttac atcgcaccta
     3481 cgccagcttg ctcagccgcc gcaatagcca ttccaagcag agcgcgccgc agatcacgcc
     3541 cagcagccag gtcatcaact gctgctcgaa gctgcggtcg ggagtgcccg gcagcggcgt
     3601 cacctggtct tgcggcttga tcaggttcag gatcgacggc cggccgccgg cgtcgcgtcc
     3661 cacggccgag gccagcccga cgaagtagtc gccgcccgac ttgtccgcga tctcccggag
     3721 caaagcgtcg ttccgctccg gggactcgct ctccttggcc ggaatcttca cgcggacctc
     3781 gcgggtcagc acctgctcgg cggctgcggg gtgctgcagc tgaatctggt aatcgccctc
     3841 ctggagggcg gtgaattgct cgacgtaaat accctcgcgg ccttcgtcct tcaccttctt
     3901 catcgtgagt gccgcgcggg tcgtgtcggg tttcacgatt accgccggaa cttcgtccat
     3961 cgtcagcggc tgatgctggg cgtcctgcaa aatagcccgc acggcgacct ggtcccccag
     4021 ggtcgcccgg tccttatcca ccagcagcac gccgcggctg gaatcgcgca gcaagcggcc
     4081 ctcggagacc cagcggatca gcttcgtgta aaactgctcg aaatagacgt cgtccacggc
     4141 tcgcacccgc cacatctcgc cgctggccat gaagaagacg cggccggagc catagaactg
     4201 tccggccatg taaatcggct ggacgttgtc gatggccgta tcgggatcgc tgaagcggga
     4261 atagacccgc gcgcccggct tgaagtcctt gacggcgtag tagcccgaga cgccctcgaa
     4321 ctggctccag gccttttcgc tggccagggc attttcgtcg agccacagga actccgcctc
     4381 cagtccgtcg cgcgtaaagg tcagcggcca ggtcttgtcg gacccaaaac ggcccaggct
     4441 gagcgtcgcc gccccttgat aatagaaggc gaccggatag agggccttga tcaggtcgat
     4501 ccgcggatcg ccgcggcggc ggctggacca ctgcggcgtg aacaccggcc ccgcgacgac
     4561 gaccaggccg cccgccttct ccgcgaccca ccgctccagc agccgcacgt ggtcttcgtc
     4621 gagctgctcc caatcggcgt cgaaggcgac gattgcgtca tactcgaaca actcgtccgc
     4681 gctatcgggg aacttgtaga gcacctcgtt ggcctcttgc gaaatgcccg gcttgcccga
     4741 ttggagccag acgtcgctga tcacttcttt gtcgcggaag agctggttcc gcaggaacag
     4801 gaagtcgcgc atcggcccgc cggcgatcaa gagcacgcgc gtccgccggt cgatgatctc
     4861 caccttggcg gtcttctcgt tgtccttcgt gtcgatctcg ccggtgaacg tggcagccgc
     4921 caggcgatac cgccgcacgc cctgcacgtc gggcttgcgc tcgaacttga ccgtcgccac
     4981 ctgccccagg cggcccacgt ccaccgtctt cgaatcggcg acggactcat tgctgccgtc
     5041 ggcttcggcc tcgtacagct tcagctcgac gctcggccgg ctgtagttct gggcctg
//
LOCUS       DASRJB010000479         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1823253, whole genome shotgun sequence.
ACCESSION   DASRJB010000479 DASRJB010000000
VERSION     DASRJB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823253"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFB80_12600"
     CDS             <1..808
                     /locus_tag="VFB80_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34656.1"
                     /translation="PVPETVHWKEFIGPAEMRPYSPAYHDFKWRGWWAFGTGALGDMA
                     CHTLNMAYHALDLRDPVSVQATTSGHNLETYPSWSIINCQFPERNGRAPLTFMWYDGG
                     KRPDTELFQKAEEAAIAKQQNQKSKDRVRGVMRSGCLVVGDKGWLLAPGDYAGDGIYM
                     HLDDIPKVEYPRSPGHFEEWVNAIKGGEQSMSNFPGYSGPLAETVLLGNLAVWVAAGE
                     QDPSKRAGQAEKGVVGKKIEWDAKTMTATNAPDVAHIIKPEFHNGYKLEG"
     gene            1011..2582
                     /locus_tag="VFB80_12605"
     CDS             1011..2582
                     /locus_tag="VFB80_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34657.1"
                     /translation="MDDRQSTLLDDLRRADSLTAQEARRSARRAAWLVECSTEQWREW
                     LTAVYELPPPLDDAADRLLATWLAAVNAERSQAARSGAPPLPPVTELLPEIQRLYHAL
                     TPVSRARAQFLPWLANSTDDAALDLLAEILVADPPADDNDVVLALAPLFQRRRREAAR
                     LFPRLLAALAHPALAAPVLDLANFLSREQLVPRHSAADQSAQLIQLLGNLVHSLEALE
                     ERQGAQGQSPQDLSQKVSRGVALAVSLCDALAQIGDKAAIGKLRQALALPHRRIRTEA
                     AAALARLGDDEGRAELIQLAAQPVARLRVLAYAAELSLLDRIDPEFQTPLARAEAELC
                     VWLAEPTQYGVPPAACELYDQRRWRWPGFDEPVDCFLFRFQYAVTLADQERSFSNIGI
                     AGPVTHAVLADLSDLPPDDIYALFAGWQAQHEEIREFEVARLARTEQLEVARLARRLN
                     DAGYAQIEPQRLGYFFGDKALVASARHQGVPGVAVADFQEIAFFPERHSQRPIGASEA
                     YWIYKGRRLLAAFNR"
     gene            2604..>3109
                     /locus_tag="VFB80_12610"
     CDS             2604..>3109
                     /locus_tag="VFB80_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34658.1"
                     /translation="MGNPTSPKIASLRPPDPHRAALLDEIVRLRTRVAQLEAAALVDR
                     APPNESPSDSPNESLVELLDRAADPQLLRRDQLLQALALGVSSATGEAFFRLLARHLA
                     HTLQCDCAVVGLLQSDRADRVRTLAFFADHDWLPNIEYELAGTPCEQVVTQDLCLYPA
                     GVQQHFPQ"
BASE COUNT          492 a         1099 c         1038 g          480 t
ORIGIN      
        1 gccagtgccc gagacggtcc actggaagga attcatcggc cctgccgaga tgcggcccta
       61 cagcccggcc tatcatgact tcaagtggcg cggctggtgg gccttcggca ccggggcgct
      121 gggcgacatg gcctgccaca cgctgaatat ggcgtatcac gccctggacc ttcgcgatcc
      181 ggtcagcgtg caagccacga cctccggcca caacctcgag acctatccga gctggtcgat
      241 catcaactgc cagttccccg agcggaacgg ccgcgcgcct ttgaccttca tgtggtacga
      301 cggcggcaag cgccccgaca ccgagctgtt ccagaaggcc gaggaagccg ccatcgccaa
      361 gcagcagaac cagaagagca aggaccgcgt ccgcggcgtg atgcgcagcg gctgcctggt
      421 agtcggcgac aagggctggc tgctggcccc cggcgactac gccggcgacg gcatctacat
      481 gcacctggac gacatcccca aggtcgagta cccgcgctcg cccggccact tcgaggaatg
      541 ggtcaacgcc atcaagggcg gcgagcaatc gatgtcgaac ttccccggct attccggccc
      601 gttggccgag acggtgctgc tgggcaacct tgccgtctgg gtcgccgccg gcgaacaaga
      661 cccgtcgaag cgggccgggc aagccgagaa gggcgtcgtc ggcaagaaaa tcgagtggga
      721 tgccaagaca atgaccgcca cgaacgcgcc ggacgtggcc cacatcatca agcccgaatt
      781 ccacaacggc tacaagctgg aagggtagtc gcccactttc gtttaacccg gagccggagg
      841 cgacggcggc tacgcgttag acgcgagatc aacactactg ccgcgagcgg accgccgggc
      901 tgctcgcggc tttttgtttc taccaagggc cagtcggtcg gtcggtcagt cggtgagtcg
      961 gtgaaccgac caaccgacca cggactgacc gaccaccgac ccaccgcccc atggatgacc
     1021 gccagtcaac gctgctggac gacctccgcc gcgccgattc gcttaccgcg caggaagctc
     1081 ggcgcagcgc gcggcgggcg gcgtggctcg tcgaatgttc cacggagcag tggcgcgagt
     1141 ggctgacggc ggtctacgaa ctgccgccgc cgctggatga cgccgccgat cgactgctgg
     1201 ccacctggct ggcggcggtc aatgccgagc gcagccaggc ggctcgctcg ggcgcgccgc
     1261 cgctgccgcc ggtgacggag ctcctgccgg agattcagcg gctctatcac gcgctcactc
     1321 ccgtttcgcg agcgcgggcg cagttcctgc cctggctggc gaattccacg gacgacgccg
     1381 cgctggacct gctggcggaa atcctcgtcg ccgatccacc ggccgacgac aacgacgtgg
     1441 tcctcgcgct cgcgccgctc tttcagcgcc gccgccgcga ggccgcgcgg ctgtttccgc
     1501 ggctgctggc ggcgctggcc catccggcgc tggccgcgcc ggtgctggac ctggccaatt
     1561 ttctgagccg cgagcagctc gtgccccggc actcagcggc cgatcagtcc gcgcagctca
     1621 tccagctgct gggcaacctc gtgcactcgc tggaggcatt ggaggagcgg cagggcgccc
     1681 agggccaatc gccccaggat ctcagccaga aagtgtcccg cggcgtggcg ctggcggtct
     1741 cgctctgcga cgcgctggcg caaatcggcg acaaggctgc catcggcaag ctgcggcagg
     1801 cgctggccct gccgcaccgc cgcatccgca ccgaggcggc tgccgctctc gcccggctcg
     1861 gcgacgacga gggccgggcc gagctgattc agctcgccgc gcagcccgtg gcccggctgc
     1921 gcgtgctggc ctatgccgcc gagctgagcc tgctggaccg gatcgatccg gaatttcaaa
     1981 cgccgctggc gcgggccgag gccgagctgt gcgtctggct ggcggagccg acgcagtacg
     2041 gtgtgcctcc cgcggcttgc gaactctacg accagcgccg ctggcggtgg cccggcttcg
     2101 acgagccggt ggattgcttt ctattccgct ttcaatatgc cgtcacgctc gccgaccagg
     2161 agcgctcgtt cagcaacatc ggcatcgccg gtccggtcac ccacgccgtg ctggccgatc
     2221 tttccgacct gccgcccgac gacatctatg ccctgttcgc cggctggcag gcccagcacg
     2281 aggaaatccg cgagttcgag gttgcccgcc tggcccgcac cgaacagctg gaagtcgcgc
     2341 ggctagcgcg gcggctcaac gacgcgggct atgcccagat cgagccgcag cggctgggct
     2401 attttttcgg cgacaaggca ctggtcgcct ccgcccgcca tcagggcgtg ccgggcgtcg
     2461 ccgtcgccga cttccaggag attgccttct tcccggagcg gcacagccag cggccgattg
     2521 gcgcgagcga agcctactgg atatacaaag gtcggaggct cttggcggcg ttcaatcgct
     2581 gacgacgccg gtcgtgccgc atcatgggaa atccaacttc accaaaaatc gcctcgctgc
     2641 ggccgccgga tccgcaccgt gcggcgctgc tcgacgagat cgtgcggctg cggacccgcg
     2701 tcgcccagct ggaggcggcg gcgctggtcg atcgcgcgcc gcccaatgag tcgcccagtg
     2761 actcgcccaa tgagtcactc gtggagttgc tcgaccgggc cgccgatccg cagctcctcc
     2821 gccgcgacca gctgctgcag gcgctggcgc tgggcgtctc gtcggcaacg ggcgaggcgt
     2881 tcttccggct gctggcgcgg cacttggcgc atacgctgca gtgcgactgc gccgtcgtcg
     2941 gcctgctgca atcggaccgg gcggaccggg tgcggacgct ggcatttttc gccgatcacg
     3001 actggctgcc gaatatcgaa tacgagctgg ccggcacgcc ctgcgagcag gtcgtcaccc
     3061 aggacctgtg cctgtatccc gcgggcgtgc agcagcactt tccgcagga
//
LOCUS       DASRJB010000480         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2954292, whole genome shotgun sequence.
ACCESSION   DASRJB010000480 DASRJB010000000
VERSION     DASRJB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954292"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..3135)
                     /locus_tag="VFB80_12615"
     CDS             complement(313..3135)
                     /locus_tag="VFB80_12615"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN34659.1"
                     /translation="MKISTFAATIAAALFLAACGSPLAGQSPPKRVPWTTSKITGAPE
                     PPRPYVIERVFPSLAFDQPVELVLIPGTNRLAVLELMGKIYSIEDQPLAQSAKRDLMA
                     DIADCDPRFFRLYGMTFHPQFAENRFCYISYVLKDRLPGCSRVSRFKMPAGDPPRIDV
                     ASEEVLLTWQGQGHNGAHLQFGPDGYLYVSTGDGGDSFPPDGRNTGQNLTDLEASILR
                     IDVDRPAEGRLYTIPADNPFVSVAAARGEVWAYGLRNPWKMCFDPADGSLWVGDVGWE
                     MWEMIYRIERGGNYGWSVVEASQPVQEERERGPTPILPPTVAHNHVESRSITGGYFSQ
                     TSRLPELRGAYVYGDYVTGKIWGLKHTGGQITWREELVDTPLQMATFGLDHAGDVLIV
                     DHPSGAIYRLAPNPRRGANDTFPRKLSETGLFADTAKHVPAAGVIPYKVNAEPWADGT
                     TAERLIALPGLAKLGVYKKTDNQVGYIAGEWQFPPDGVLAKTVSLGARRLETQVLHFD
                     VDAWKAYNYLWNEEQTDAVLADDVSSESVARDSVASTQGGVAGYKWHHASRTECILCH
                     TTRVGTVHGFRLPQLGSEADRLDALGLFAEPLPRKRDVIPDPHDAGQTLEARARAYLH
                     VNCGHCHSRGGGGSSFFDVRWEIPTEKARLFLRPTQGAFGIAGAGIVAPGDPYHSVLY
                     YRMCKLGRGHMPQFGSQLIDLRGTRLIHDWIASLPATGPITPPPAQTWEQLLATPSGA
                     LQVLTMLDAGKLPVSEAVAAIARGAAHSDPTIRDLFERFMPEEKRVKRLGSAVKSEAI
                     LSLAGDATRGRELFLNATGVACKNCHRIGQEGKPLGPDLTQIGKKLDRPKLLESILEP
                     SKTVEPNFTSYLVESDGQVLTGLIAQRDPTTLVLRQADGKEVRLATSEIDRLAPTAKS
                     LMPELLVQEMTAQQVADLLEFLAGLK"
     gene            3168..4085
                     /locus_tag="VFB80_12620"
     CDS             3168..4085
                     /locus_tag="VFB80_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34660.1"
                     /translation="MLRAEPGTNMQRLLISTRAESECHEIARRLRGASAVWNGGRPPR
                     EPFAEGFDAVAFPSPNADTPAELALGVRAGKHLLLVDPWQVPEEAVTWMFTQARAAGV
                     RFVVANYDRYWPSRRLIHQELVAGKLGRPGLVRIHRWWQLALDDSRLGQRLPPGLSRD
                     LDLAIWLAGARPDVAYACGDIANVGAYLQVHLGFDGGGMALIDYCTRLPEDSRGYQSL
                     HVIGSSGAAYCDEQQQVQLSFGGGLPRAVIGRDSYVVPSIVQDFVDDTPAEASLAMSY
                     AQWQHVRAAAEAVRQSLATRKAVRVEAVA"
     gene            4082..>4408
                     /locus_tag="VFB80_12625"
     CDS             4082..>4408
                     /locus_tag="VFB80_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34661.1"
                     /translation="MSAPGKFRCAVLSAVKHDYVARGMASHPRFELAVVADDPQVPAW
                     AHERNQKLADEFRIPYVQDIEQALAEFDVQVAIVSPEAERHCDLSARAAAAGRHVVQD
                     KPLATCR"
BASE COUNT          742 a         1387 c         1499 g          780 t
ORIGIN      
        1 tcgtcgtcag cgccgacttg aggaaggcgt cggaagagga gctgaagcgg gcgaaggtgg
       61 agatgacgat cgtgggtggg aaggtcgttt accgcggcgc tggcgagtag aacggcgacc
      121 gggcaacgag caagagaaag atgaagatta ggattaggaa gatgattaag agcaagatta
      181 ggagaatgag taggatcgag ccgtctgagt cctgctcttg atctttatct tgctcctaat
      241 cctattctta atcttactcc tcctcttaat ctttctctga gtgctagtgc gagactgcct
      301 cccaagacaa cgctacttca gccccgccaa aaactcgagc aaatccgcga cttgctgggc
      361 ggtcatctct tgcacgagca attccggcat cagtgatttc gccgtcgggg cgaggcggtc
      421 gatttcgctc gtggccaggc ggacttcctt gccgtccgcc tgccgcagaa cgagcgtggt
      481 cggatcgcgc tgggcgatga ggcccgtcag cacctggccg tccgactcga ccaggtagct
      541 ggtgaagttc ggctccacgg tcttcgacgg ctccagaatg ctctccagca gcttcggccg
      601 gtcgagcttc ttgccgatct gcgtcagatc ggggccgagc ggcttgccct cctgcccgat
      661 gcggtggcag ttcttgcagg ctacgccggt ggcattcagg aacagctcgc ggcctctggt
      721 cgcgtcgccc gccagcgaca ggatcgcctc cgacttgacc gcgctaccga ggcgcttgac
      781 gcgcttctct tccggcatga accgctcgaa caggtcgcgg attgtgggat ccgagtgggc
      841 cgccccgcgg gcaatggccg ccacagcctc gctgactggc aatttgccgg catcgagcat
      901 tgtcagcact tgcagtgcgc cgctgggagt cgccagcagc tgctcccagg tttgggccgg
      961 cggcggcgtg atcgggcccg tagccggcag cgaggcgatc cagtcgtgga tcagccgcgt
     1021 gccgcggaga tcgatgagct gcgagccgaa ctgcggcatg tgcccgcggc ccagcttgca
     1081 catccggtag taaaggaccg agtggtacgg atcgccgggt gcgacgattc cggcccccgc
     1141 gatgccaaac gcgccctgcg tcggccgcag aaagaggcgg gctttttccg tggggatctc
     1201 ccaccgcacg tcgaagaacg acgagccgcc gccgccccgc gagtggcagt ggccgcagtt
     1261 gacgtgcaga taggcccggg cccgggcctc cagcgtttgc ccggcatcgt gcgggtcggg
     1321 aatgacatct cgtttccgcg gcagcggctc ggcgaacagg cccagtgcat ctagccggtc
     1381 cgcttcgctg cccagctgtg gcagccggaa gccgtggacg gtgccgacgc gggtcgtgtg
     1441 gcagaggatg cactcggtgc ggctggcgtg atgccacttg tagcccgcga ctccgccctg
     1501 ggtgctcgcg acggagtcgc gagctacgga ttcgctcgac acatcgtcgg cgagcaccgc
     1561 gtcggtctgc tcctcgttcc agagatagtt gtaggccttc caggcatcga cgtcgaaatg
     1621 caggacttgc gtctccagcc gccgcgcgcc gagcgagacc gttttggcca gcacgccgtc
     1681 cggcggaaac tgccattcgc cggcgatgta gccgacctgg ttgtcggtct tcttatagac
     1741 gccgagcttc gccaggccgg ggagggcgat taagcgctcg gcggtcgtgc cgtcggccca
     1801 cggttccgcg ttgaccttgt acggaatcac gcccgccgcc ggaacgtgct tcgccgtgtc
     1861 ggcaaacagg ccggtctcgc tcagcttccg cgggaaagtg tcgttcgccc cgcggcgcgg
     1921 attgggcgcg agccgataga tcgcaccgga cggatgatcg acgatcagca cgtcacccgc
     1981 gtgatccagg ccgaatgtgg ccatctgcag cggcgtatcg accagctcct cgcgccaggt
     2041 gatctggccg ccggtgtgct tgaggcccca gattttgccg gtgacgtagt cgccatagac
     2101 gtacgccccg cgcagctccg gcagtcgcga ggtttgcgaa aaatagccgc cggtgatcga
     2161 acgggactcg acgtggttgt gggcgaccgt cggcggcagg atcggcgtgg gccctcgctc
     2221 gcgctcttcc tgcaccggct gcgaagcctc gacgacactc cagccgtagt tgccaccccg
     2281 ctcgatgcgg tagatcatct cccacatttc ccagccgacg tcgccgaccc acagcgagcc
     2341 gtcggcggga tcgaagcaca tcttccacgg gttccgcagg ccataggccc agacctcgcc
     2401 gcgggcggcg gcgacgctca cgaacggatt gtcggcgggg attgtgtaga gccggccctc
     2461 cgccggacga tcgacgtcga tccgcaggat cgaggcctcc aggtcggtca ggttttgccc
     2521 ggtgttgcgg ccgtcgggcg gaaagctgtc gccgccgtcg ccggtcgaga cgtagaggta
     2581 gccgtcgggg ccaaattgca ggtgcgcacc gttgtgccct tggccttgcc aggtgagcag
     2641 cacctcttcc gacgccacat cgatccgcgg cgggtcgccc gccggcattt tgaaccgcga
     2701 cacgcggctg catcccggca ggcggtcctt cagcacgtat gagatgtagc aaaagcggtt
     2761 ctcggcgaac tggggatgaa acgtcatccc gtacagccga aagaaccgcg ggtcgcagtc
     2821 cgcgatatcg gccatcaagt cgcgctttgc actctgggcc agcggctgat cctcgatcga
     2881 atagatcttg cccatcagct ccagaacggc gagccggttc gtgcccggaa tcaggacgag
     2941 ctcaaccggc tgatcgaagg cgagcgaggg aaagactcgc tcgatgacgt acggccgcgg
     3001 cggctccgga gcgccggtga tcttcgaggt cgtccaggga acgcgctttg gcggcgactg
     3061 gccagcaagc ggcgatccgc aggccgccag gaacaaggct gccgcgatcg tcgcggcgaa
     3121 agtgctgatc ttcatagccg ccgattgtac caacggccaa aggcgatatg ctgcgtgccg
     3181 aaccaggcac aaacatgcag cggctgctaa tcagcacgag ggctgagtcg gaatgtcacg
     3241 agattgcgcg gcggctgcgc ggggcgagcg cagtctggaa cggtggccgt ccgccgagag
     3301 agccgtttgc tgagggattc gatgcggtcg cctttccatc gccgaatgca gacacgcctg
     3361 ccgaactggc cctgggtgtg cgggccggca agcacttgtt gctcgtcgat ccctggcaag
     3421 tcccggagga ggcagtgact tggatgttca cccaagctcg ggcggccggc gtgcgattcg
     3481 tcgtggccaa ttacgaccgc tattggccgt cgcgacggct gattcaccag gagctggtgg
     3541 ccggcaagct gggccgcccg ggcctggtgc gaattcaccg ctggtggcaa ctggcgctgg
     3601 acgattccag gcttggtcag cgcctgcccc cgggtctgtc gcgagacttg gacctggcca
     3661 tctggctggc tggcgcgcgg ccggacgtcg cctatgcctg cggcgacatt gccaacgtcg
     3721 gcgcatatct ccaagtccac ttgggctttg acggcggcgg gatggcgctc atcgactatt
     3781 gcacccgctt gccagaagac agccgtggct accaatcgct gcatgtcatc ggctcttcgg
     3841 gggcggccta ttgcgacgaa cagcagcaag tgcagctctc gtttggcggc ggtttgccga
     3901 gggccgtcat aggtcgagac agctacgtgg tgccatcgat cgtgcaggat tttgtcgacg
     3961 acacaccagc cgaagccagc ctcgccatga gctacgcgca gtggcagcac gtgcgggctg
     4021 ccgccgaggc ggttcggcaa tcgctggcca cgcggaaagc cgtgcgggtg gaggccgtcg
     4081 catgagcgcg ccaggcaagt tccgctgcgc cgtcttgagc gccgtcaagc acgactatgt
     4141 cgcccgcggg atggcgtcgc atccgcgatt cgagctggcg gtggtggccg acgatccgca
     4201 ggtgcccgcg tgggcgcacg agcggaatca gaagctggcg gacgagtttc ggattcctta
     4261 cgtccaggac atcgagcagg cgctggccga gttcgatgtg caggtagcca tcgtcagccc
     4321 ggaggcagag cggcattgcg acctgagcgc gcgggccgca gcagcgggca ggcacgtcgt
     4381 gcaggacaag ccgctggcga cctgccgg
//
LOCUS       DASRJB010000481         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4082496, whole genome shotgun sequence.
ACCESSION   DASRJB010000481 DASRJB010000000
VERSION     DASRJB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082496"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..492
                     /locus_tag="VFB80_12630"
     CDS             223..492
                     /locus_tag="VFB80_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34662.1"
                     /translation="MSRWNRLLAAAVLLALLASSPAAQAGPPAYLLLRRAESPGRHFH
                     AGHADAAMYDARASGYAYGYFGAAPRSHFSRHYGYYDTYKQWSRW"
     gene            complement(757..2319)
                     /locus_tag="VFB80_12635"
     CDS             complement(757..2319)
                     /locus_tag="VFB80_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZN34663.1"
                     /translation="MALARHNSPLIAACWLTAALAGCASPCPWGPPCNGGACGPPNVP
                     IVVPPASAAPAAAPGVTVRGQSPGGYYGSETMPGGYPRGPQPVSGGQPQTVSPYGQPP
                     AGNAAAYPGYNDPQMTGYQPPGVDPSLTGPPLPAPPAEAMPGGPITPAYPGFMPPSNI
                     IPPGTPTPLDVFVEEARTGRFMFGVAVNSDAGVTGQITIDERNFDYTRWPGSWDDFAT
                     GGALRGAGQGFRVELQPGSQVQRYLISFTDPYFWNDISFSASAFYFDRRYFDWNESRY
                     GGRLAWGYRLTPDLSVTAAAGAQNVNIFEPRVTGVPELDAALGRSDLFSGRISLTHDT
                     RDLPFMPTEGHLFELSYEQVFGSFDYPRGEVNYSQYFMVRQRPDGSGRHTLAFSTRAG
                     FSGSQTPIYENYFAGGFSSLRGFAFRGASPVDMGVRVGGEFQFINSAEYFFPLTADDM
                     IRGTLFCDFGTVETDIAIRPDNFRVAPGFGFRISVPALGPAPLAFDFAFPVAYAATDQ
                     RQTFSFFFGAMR"
     gene            complement(2355..>3770)
                     /locus_tag="VFB80_12640"
     CDS             complement(2355..>3770)
                     /locus_tag="VFB80_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZN34664.1"
                     /translation="LAIFLACGAVASGQDLSQSFPAPGGPMPQAPQPFQPAPRQLVVE
                     VVLAGNFFTKEYDIQKHIHTRKDREFDPEVVQADVRRLVTSGLFRDVKTKLRQEPGGV
                     VVIFELEERPRIHEVKFLGNRGVSEKKLNKEIGAKAGDPLNTYLAEESRRKIEELYHH
                     EGYPLASVNLLEGGRPGDKNLTFEIVEGPVQRIFRVAFENNTIASDGRLKTQIQSKPG
                     WAWYFFGGKVDRDKIDADREKLEIYYRNLGFFKARVSRRLDADASGKWVTLRFIIDEG
                     PRYRVRNVSIEGNAKFASQPLLDFLELKSGAFYNQSLMTRDLNTLTDLYGSQGYVFAD
                     VQADPRFLEEPGLLDLVYRVKEGEVFRVGEIHVNIAGEFPHTRQTVILNRLGFGPGEI
                     IDMRKVKNAERRLLASQLFAGGPSAPGDPPRITVRPPEFGSLVRGQEPEATWPAAPAD
                     APRLPESPWRPPPGPPPFAPR"
BASE COUNT          649 a         1245 c         1261 g          615 t
ORIGIN      
        1 ggacttccgc ggcgcgtcgc tgctgggaac gaccttcacc agcgccggtt tcgactacgc
       61 aacgcaattc gacctggacc gactcgatga cctcgcgccg ggcgaggccg agttcatccg
      121 cgccagcctg ggccgctagc aacgcatttc cgcgcggccg gcggaacttg aactgccggc
      181 ccggtcgcgg ccgcgccgat ggaactggaa cgagggctcg ccatgtccag atggaaccga
      241 ctgctcgcgg ctgccgtact gctggctctg ctggcgtcca gcccggcagc gcaggccgga
      301 ccgccggcat acctgctgct ccgccgcgcc gaatcgccgg gccggcactt ccacgccggc
      361 cacgccgacg ccgcgatgta cgacgcccgc gccagcggct acgcctacgg ctacttcggg
      421 gctgccccgc gctcgcactt ctcgcggcac tacgggtatt acgataccta caagcagtgg
      481 agccggtggt agccagcgcc accggcaaga ttgtgtacat tggtacgtaa tttgctcaat
      541 agttccaagt gaggcatggg gctgccattt tggcccgcgc ttcgcgcggg cacgagcacc
      601 gtagcgagcc cagaatcggg cctcaaacac tcatatataa ctacttaccg aggtggtcgg
      661 gatttgaccc aagtcgaaga gccgccgaac tttgtggcga acgaggcgaa ttgcaagcca
      721 ccttaccggg gggcgccttg atgagaacgc cggtccttac cgcatcgcac cgaagaagaa
      781 gctgaacgtc tgccgttgat cggtggcggc gtaggcgacc gggaacgcga agtcgaacgc
      841 cagcggcgcc gggcccaggg ccggcacgct gatgcggaag ccaaagcccg gagcgacgcg
      901 gaaattatcc ggccggatgg cgatgtcagt ctcgaccgtg ccgaagtcgc agaacagcgt
      961 gccgcggatc atgtcgtcgg ccgtgagggg gaagaaatac tcggccgagt tgatgaactg
     1021 gaattcgccg ccgacccgca cacccatatc gacgggcgag gctccccgga acgcgaaccc
     1081 gcggagtgac gagaagccgc cggcgaagta attctcgtag atcggcgtct gcgagccgct
     1141 gaagcccgct cgcgtggaga acgccagcgt gtgccggccg gagccgtcgg gccgctgccg
     1201 caccatgaag tactgcgaat agttcacctc gccgcgggga taatcgaacg agccgaatac
     1261 ctgctcgtag gacagctcga acaagtgccc ctcggtcggc atgaacggca agtcgcgggt
     1321 gtcgtgcgtc aggctgatgc ggccgctgaa caggtcgctg cggccgaggg ccgcgtcgag
     1381 ctccggcact ccggtcacgc ggggctcgaa gatgttcacg ttctgcgccc cggccgccgc
     1441 cgtcaccgac aggtcgggcg tcaggcgata gccccacgcc aaccggccgc cgtaccgcga
     1501 ctcgttccag tcgaaatacc ggcggtcgaa atagaacgcg ctggcactga agctgatgtc
     1561 gttccagaaa tacgggtcgg tgaagctgat caggtaccgc tgaacctggc tgccgggctg
     1621 aagctcgaca cggaagccct gaccagcgcc gcggagcgcc ccgccggtgg cgaagtcgtc
     1681 ccagctcccc ggccagcgcg tgtaatcgaa gttccgctcg tcgatcgtga tctgcccagt
     1741 gacgcccgcg tcggagttca ccgccacgcc gaacatgaat cggccggtgc gggcttcctc
     1801 gacgaacacg tccagcggcg tcggcgttcc cggcgggatg atgttcgacg gcggcatgaa
     1861 gccgggataa gccggtgtga tcggaccgcc cggcatcgcc tcggctggcg gcgcaggcaa
     1921 tggcggaccc gtcagtgacg gatcgacgcc cggcggctga tagccagtca tctgcggatc
     1981 gttgtagcct ggatacgctg cagcgtttcc ggcgggcggc tggccatagg gcgaaaccgt
     2041 ctgcggctgc ccgcccgaca ccggctgcgg accgcgcgga tagcctccgg gcatcgtctc
     2101 gctgccgtaa tagccgcccg gcgattggcc gcggactgtc actcccggcg cggccgcagg
     2161 cgcggcagag gccggcggca ctacgatcgg cacattcggc ggcccacagg cgccgccatt
     2221 gcacggcgga ccccacgggc acgggctcgc gcagcccgct agcgcggccg tcagccaaca
     2281 ggcggcgatc agcggcgaat tgtggcgggc aagtgccaag gcgggaatct cggcaatcct
     2341 cgaagtgtct ggttttagcg cggggcaaac ggtggtgggc caggcggcgg tcgccacggc
     2401 gattcgggca atcgcggcgc gtcagcaggc gccgccggcc aggtcgcctc cggctcctgc
     2461 ccgcggacga gcgagccgaa ttccggcggc cggacggtga tccgcggcgg atcgcccggt
     2521 gcgctggggc cgccggcaaa cagctgcgag gccagcagcc ggcgttcggc gtttttgacc
     2581 ttgcgcatgt cgatgatctc gccggggccg aagccgagcc ggttcaggat gaccgtctgc
     2641 cgcgtgtgtg gaaattcgcc ggcgatgttg acgtggatct cgccgacgcg aaagacttcg
     2701 ccttccttga cgcgatacac cagatccaag aggcccggct cttccaggaa gcgcggatcg
     2761 gcctggacgt cggcgaagac gtagccctgg ctgccgtaca agtcggtgag cgtgttcaga
     2821 tcgcgggtca tcagcgactg gttgtaaaac gccccggatt tcagctccag gaaatcgagc
     2881 agcggctgac tggcgaactt cgcgttgcct tcgatcgaga cgttgcggac gcggtaccgc
     2941 gggccttcgt cgatgatgaa ccgcagcgtg acccacttgc ccgaggcatc ggcgtcgagc
     3001 cgccggctga cgcgggcttt gaagaagccc aggttgcggt aatagatttc cagcttctcg
     3061 cggtcggcgt cgatcttgtc ccgatcgacc ttgccgccga aaaagtacca ggcccagccg
     3121 ggcttcgact ggatttgcgt cttcagccgg ccgtcgctgg cgatggtgtt gttttcgaag
     3181 gcgacgcgga agatccgctg caccggccct tcgacgatct caaacgtgag gttcttgtcg
     3241 ccaggccggc cgccttcgag caggttcacg ctcgccagcg gatagccctc gtggtgatac
     3301 agctcctcga tcttgcggcg cgactcctcg gccaggtaag tgttcagcgg atcgccggcc
     3361 ttggcgccga tctccttgtt gagctttttc tcggagacgc cgcggttgcc gaggaacttc
     3421 acctcgtgaa tccgcggccg ctcttccaat tcgaagatca cgaccacgcc gcccggctcc
     3481 tgccgcagct tggtcttcac gtcgcggaac aggcccgacg tgaccagccg ccgcacgtcg
     3541 gcctgcacga cctcgggatc gaactcgcgg tccttgcggg tgtgaatgtg tttctgaatg
     3601 tcgtattcct tggtgaagaa gttgcccgcc aggacgacct ccaccaccag ctgccgcggc
     3661 gccggctgaa agggctgcgg cgcttgcggc atcggccctc ccggagccgg aaacgactgc
     3721 gagaggtcct gcccgctcgc taccgcgccg caggcaagga aaatcgccaa
//
LOCUS       DASRJB010000482         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_983594, whole genome shotgun sequence.
ACCESSION   DASRJB010000482 DASRJB010000000
VERSION     DASRJB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983594"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFB80_12645"
     CDS             <1..194
                     /locus_tag="VFB80_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34665.1"
                     /translation="APLAEVSRHPTNPNVFGLKNASSGRWVATTADGAVKDVEPGKSI
                     VLAGGTRIAFGRQTGELVQ"
     gene            complement(188..961)
                     /locus_tag="VFB80_12650"
     CDS             complement(188..961)
                     /locus_tag="VFB80_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34666.1"
                     /translation="MRDKLCRMVARFGAAVIDDARRCEALLKDFCPEDRREVHVLMAA
                     VREQVAADLVAPTIKQKPAAAIGRLTKRLEENLGLSAEAARWAVESWALALGVVKPQQ
                     LAAARPQKPPAPAVAKQVAAPPVIVKRPANAIAPLKSPRRGWLIALSLAALVLAPGAG
                     VGLAWSSGAMRGMRSRTSEALPQRQAVEATTPAAMSVESSSPETAEPPERPAAIEFAS
                     ALAVQPRAASMDPISPEEEQWVRDVLKRSAPAGSAGRDY"
     gene            1091..2035
                     /locus_tag="VFB80_12655"
     CDS             1091..2035
                     /locus_tag="VFB80_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZN34667.1"
                     /translation="MDKLQAKPTEPIAWLNGRWIPLSQAAVSVFDAGFVQGVTVAEQL
                     RTFGGKLFRLQMHLERLAQSLGIVGVDPGLSTTELAELAEAVAARNFQLLDPADDLNL
                     TIFVTPGPYGAYAAIAEHKAPSVCLHAQSLPFGQWAEKYRTGESLVVSDVQQVPASCW
                     PAVLKCRSRMHYYLADKQARQKEAGARALLLDERGFVTEASTANVLVYDAKHGLLSPP
                     QENILPGVTVAVIEELAGQLGIPFQHRDLAVDDVARAAEVLLCSTSPCLLSVTRLNGR
                     PVGDGRPGPISARLRQAWNEMVALDIEAQAKRFSTRPV"
BASE COUNT          362 a          775 c          731 g          410 t
ORIGIN      
        1 ttgccccgct ggccgaagtc agccgccatc ctacgaatcc caacgtcttt ggcctcaaga
       61 atgcgagcag cggccgctgg gtcgcgacga ccgccgacgg cgcggtgaag gacgtcgagc
      121 ccggcaagag catcgttttg gccggcggca cgcggatcgc cttcggccgc cagaccggcg
      181 agctggttca atagtcccgg ccggcgctgc ctgccggcgc gctccgcttg agcacgtcgc
      241 gcacccactg ttcctcttcg gggctgatgg ggtccatgct cgctgctcgg ggctgcaccg
      301 ctagtgcgct tgcgaactcg atggccgccg gccgctcggg cggttccgca gtctcgggcg
      361 agctggattc aacgctcatt gcggccggag tcgttgcctc tacagcctgc cgttgcggaa
      421 gggcctcgct ggtccggctg cgcatgcctc gcatggcgcc cgagctccaa gccagcccca
      481 ctccggcgcc cggcgcgagg acgagcgccg ccagcgacag cgcgatcagc cagccgcggc
      541 gcggcgactt cagcggcgcg atggcattgg ccggccgctt tacgatcacg ggcggggccg
      601 cgacttgctt cgcaactgcc ggcgctggtg gtttctgcgg ccgggctgcg gccaattgct
      661 gcggcttgac gacgccgagg gccagcgccc acgattccac cgcccagcgg gccgcttcgg
      721 ccgagagtcc caggttctct tccagccgct tcgtcaggcg gccgatcgcc gccgcaggtt
      781 tctgcttgat tgtcggtgcg accaggtccg ccgcgacttg ctcccgcacc gcggccatca
      841 gcacgtgcac ctcgcgcctg tcctcggggc aaaagtcctt gagcagtgcc tcgcaccgac
      901 gcgcatcgtc gatcaccgcc gccccaaacc gggcgaccat ccggcacagc ttgtctcgca
      961 cgctcgggct catcgcacac cccggctttc atccctgcca agcgcatttg cggcgaaaat
     1021 gtgactcgcc aaagtccagg tacaatggga gcaagcgaat tggacatttt ccggcgggat
     1081 ggagttgttg atggataaat tgcaggccaa gccaaccgag ccgattgcct ggctgaacgg
     1141 ccgctggatt ccgctgtccc aggcggccgt gagcgtgttc gacgcgggct tcgtgcaggg
     1201 tgtaaccgtt gccgagcagc ttcgcacatt cggcggcaag ctgtttcgcc tgcagatgca
     1261 tctggagcgg ctcgcccagt cgcttgggat cgtcggcgtc gatccgggcc tctcgacgac
     1321 cgagctggcc gaactcgctg aggcggtcgc cgcgcggaac ttccagctgc tcgatccggc
     1381 cgacgacttg aacctgacga tcttcgtcac gcccggcccg tatggcgctt atgccgcaat
     1441 cgcggagcac aaggcgccgt cggtatgcct gcatgcgcag tcgctgccgt ttgggcaatg
     1501 ggccgaaaag taccgcaccg gcgaatcgct cgtggtgagc gacgtccagc aagtgcccgc
     1561 cagttgctgg cccgcggtgc tgaagtgtcg cagccggatg cactactatc tggccgacaa
     1621 acaggcccgc cagaaagagg ccggcgccag agcgttgctc ttggacgagc gcggctttgt
     1681 caccgaggcc tccaccgcca atgtgctggt ttacgacgcg aaacatggcc ttctttctcc
     1741 gccgcaggag aacatcctgc ccggtgtcac ggtcgccgtg atcgaggagc tggcgggcca
     1801 gctgggcatc ccgtttcagc accgcgacct ggcggtggac gacgtggccc gcgccgccga
     1861 agtgctcctg tgcagcacgt ctccctgtct gctctccgtc acgcggctga atggccgccc
     1921 ggtcggcgac ggccggcccg gacccatttc cgcccggctg cggcaggcct ggaacgagat
     1981 ggtcgctttg gacatcgaag cgcaggcgaa gcggttttca acgaggcccg tatagcgcct
     2041 cgagtgcaac cccctgaatt ggtagcctga acttgccctt cgcttgccaa tttcgccaca
     2101 aggctttgca gtggaatgac ttgtgacgcg atcccgaaaa gcccataaca actacttatt
     2161 agtgtttgag accggctgtt tcgtgtcgcc gcacctgggg gatttggcgg ctaagggcga
     2221 atcagaatta acgtggcgaa tccgcctcac cgcgatctcg ctaacgcgag aaccgcca
//
LOCUS       DASRJB010000483         4585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1260168, whole genome shotgun sequence.
ACCESSION   DASRJB010000483 DASRJB010000000
VERSION     DASRJB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260168"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VFB80_12660"
     CDS             <1..1295
                     /locus_tag="VFB80_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZN34668.1"
                     /translation="PGVTSAGSSILLNGINQYVALPAAPFGDPAAPGFSASFETWFRA
                     APGTSGVILSQTAAGATPGGAIPAGYVPVVHLGSDGRVRSTMFWHGDANARVVSPEAI
                     SYNDGRWHHVAVTYAAGVETLYLDGVAAGQQVRPEVPYAAAYNYYLGTGQTSLWAGGN
                     GGWHFFSGQLDEAAIYPRALSPAEVAAHFAAASQATAAAWYRFDESSGAAAADSSGNG
                     QTAAYVNQPVLAQPGAASGGTAASLNGINQYVALPAAPFGNAAAGPLAFETWFQATPG
                     TSGVILSQTAAGATPGGATPPGYVPVVHLGTDGRIRSTMFWHGDAGARIVSPSAYNDG
                     LWHHVAVTYAGGIETLYLDGATIGQQAQPEIAYAAAYNYFLGTGQTSLWAGGNGGWHF
                     FNGRLDEAAIYDRALTSGEIAVHAALDPAAVDAVMGGG"
     gene            1636..2271
                     /locus_tag="VFB80_12665"
     CDS             1636..2271
                     /locus_tag="VFB80_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HZN34669.1"
                     /translation="MPRVSATTSAAEFLPHRRNLPSLRKAAETCRGCPLYANATQTVF
                     GEGPNTARAVFVGEQPGDVEDRAGRPFVGPAGRLFDELLDAAGIDRRQIYVTNAVKHF
                     KWKPQGKRRLHAKPSSREIFACRPWLQAELEAIEPELLICLGATAAQSLLGRGFKLTQ
                     RRGEPFASDWADWTLATYHPSAVLRAKHLEGGEILVREFQADLELVAAKLK"
     gene            2359..4377
                     /locus_tag="VFB80_12670"
     CDS             2359..4377
                     /locus_tag="VFB80_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34670.1"
                     /translation="MPAFFRARSSRTLVLTAALCLSAAATLLAEPQDRQVAEWVLLMG
                     GSVGIEGQAERVRDIAALPQGDFRLQLVDLVGTNILPPDLKWLTGLTRLRVLKLPGPM
                     WNPSSGATIEYSRNLKHIAGIPTLEELTFSYTYLESIKFDDTGIEEIAPLAPSLKLLS
                     LENTQVRGRHLGKFTNLQSLDLVYCPINDEGLKQIGGLTKLRQLYLRDAVISDEGLAS
                     LKDLTRLEQLDLGGTRIGDAGATHLRGMTKLKKLSLQGAVLTDAGLRNLAGLTALEEL
                     NLYGTKVTNSGVDVLENMQRLRTIDLRYSRLTRAGVDRLRAAVPQCQVTFLGASVRPA
                     IPAGAERTVSGDDSAVAQWVRAIGGKATLDGGRLMAVDLATTSVTDDLLRNLRGLAHL
                     RKLQLGSTEIGDPGLEYLAGLTTLMELDLRGTAISDAGLAHLAGLTSLRQLGLADTQI
                     TGGGLKHLASLPLEVLRLSNAPVGDEGLAHLGKMASLRELALAFTDVTNDGLAQLAPL
                     AGLQRLDLAGTDVGDNGLAHVGQLTGLTGLALDYTRITDAGVPRLEKLTGLTQLGLTR
                     TRVTDKSMGTVGRLTRLSELKLDYTDVGDEGLAALKDLAQLARLSLDSTNVTDASAPR
                     LAEFKQLRQLNLYHTFYTDKGREQLRAALPQCEVIFDARSSDPKRRRS"
     gene            4390..>4585
                     /locus_tag="VFB80_12675"
     CDS             4390..>4585
                     /locus_tag="VFB80_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34671.1"
                     /translation="MTPTRRASEGVLFLALLTPPLSLAAADQAVLAELDALGARTTRN
                     EAGEVTAVDLTNAWLSDADLA"
BASE COUNT          771 a         1593 c         1466 g          755 t
ORIGIN      
        1 aaccgggcgt aacgtcggcc gggtcgtcga tcctcctgaa cgggatcaat caatacgtcg
       61 ccctgcctgc cgcgcccttt ggcgatccag cggcccccgg cttctccgcc agcttcgaga
      121 cctggtttcg ggccgcgcct ggcacgagcg gcgtgatcct gagccaaacg gcggccggcg
      181 ctacgcccgg cggcgcgatt cccgccggct atgtgcccgt cgtccacttg ggcagcgacg
      241 gcagggtccg ttcgacgatg ttctggcacg gcgacgccaa tgcgcgggtc gtctcgccgg
      301 aggctatatc ctataacgat ggccgttggc atcacgtggc cgtcacgtat gccgcgggcg
      361 tcgaaacgct gtacctggac ggcgtggccg cggggcaaca ggttcggccc gaagttccct
      421 acgccgcggc ttacaactat tacctcggga ccggccagac atcgctgtgg gccggcggca
      481 acggcggctg gcatttcttc agcggccagc tcgacgaggc cgccatctat ccgcgggcgc
      541 tttctccggc agaagtcgcc gcgcactttg ccgcggccag ccaggcgact gcggcggctt
      601 ggtatcggtt cgacgagtca agcggcgcag cggccgccga ctcctcaggc aatggtcaga
      661 ccgccgcata cgtcaatcaa ccggtgctgg ctcagcccgg cgcggcctcg ggcggaacgg
      721 ccgcgagctt gaatggcatc aatcaatatg tcgcccttcc agccgcgcct tttggcaacg
      781 cggcagcggg tcccctcgcg ttcgaaacct ggtttcaggc gacgcctggc acgagcggcg
      841 tgatccttag ccagacggca gccggagcca cacccggcgg cgcaactcct cccggatacg
      901 tcccggtcgt gcatctggga accgacggca gaattcgctc gacgatgttc tggcacggcg
      961 atgccggggc gcgcatcgtc tcgccgtccg cttacaacga cggcctctgg catcacgtgg
     1021 ccgtcacgta cgccggcggc atcgaaaccc tctacctgga cggtgccacc atcggccagc
     1081 aggcgcagcc cgaaattgcc tacgcggcag cttacaacta tttcctgggg acgggccaaa
     1141 cctcgctgtg ggccggcggc aacggcggct ggcacttttt caacggccgg ctcgacgaag
     1201 cggctatcta cgatcgtgca ctcacctccg gcgagatcgc ggtgcacgct gctctcgacc
     1261 cggcagccgt tgatgccgtc atggggggcg gatagtcaag aaacaaagcg accggccacc
     1321 gtcgcacttc ctgtctgcat cctgccgagt tcacgcttgt caagtctctc ggtatgaacc
     1381 aagtgattcg ttgactagct gatttttctc tgcaagttgt ttcaggtttg cggcagttgc
     1441 cggtctgtga gaagagtgtc gcaacctccg tgcccggcgt cgcgaaattt gcccgcctga
     1501 attctggcgt gttccacaac ggtcattcgc aaggaaacgc gccagccatc gcgacactct
     1561 ggccgctgcc gcgtcaatgc tgctagcagc cgtcgtcgcg aactatctgg cgcggttgtt
     1621 gcttcctgaa tcaccatgcc gcgcgtttcc gctacgacat cggccgccga gttcctcccg
     1681 caccgccgca atctgccctc gctcaggaag gcggccgaga cctgccgggg ctgtccgctg
     1741 tatgccaacg ccacgcaaac ggtatttgga gaagggccga acacggcccg ggccgtgttc
     1801 gtgggcgagc agcccggcga cgtcgaagac cgcgccggtc ggccgtttgt tggacctgcc
     1861 ggtcgattgt tcgacgagct gctggacgcc gccggaatcg accgccggca aatctacgtc
     1921 accaacgccg tcaagcactt caagtggaag ccgcaaggca agcggcgatt gcacgccaag
     1981 ccgtcgtcgc gcgaaatctt tgcctgccgc ccctggctgc aggcggagct cgaagccatc
     2041 gagcccgagc tgctgatctg cctcggagca accgccgctc agtcgctcct gggccgcgga
     2101 ttcaaactca cacagcggcg cggggagccg ttcgcatccg attgggccga ctggacgctc
     2161 gccacctacc atccctccgc cgtgctgcgg gccaaacatc tggagggtgg ggagatcctg
     2221 gtccgcgagt ttcaggcgga cctggagctg gtcgcggcga aactcaagta ggccgaagtc
     2281 ctgcccgctg agactattcc gacgcgtgcg aatcgaccat attggcggca tggtttctgg
     2341 tcccgcggag gccgagtcat gcccgctttc ttccgagccc gctcgagccg cacgttggtg
     2401 ctcacggcgg cactttgcct gtccgcagcc gccacgctgc tggctgagcc gcaggatcgg
     2461 caggttgccg aatgggtgct gctgatgggc ggctcggtcg gcatcgaggg ccaggcggag
     2521 cgggtgcgcg acatcgccgc gctccctcag ggggactttc gcctgcagct cgtggacctc
     2581 gtcgggacga acatccttcc gccggacctc aagtggctga ccggcctgac gcgcctccgc
     2641 gtcctcaagc tgcccgggcc gatgtggaat cccagctccg gcgccacgat cgaatacagt
     2701 cgcaacctca agcacatcgc gggcattccc acgctggaag aattgacctt cagctacacc
     2761 tacctggagt cgatcaagtt cgacgacaca ggcatcgagg agatcgcgcc cctggcgccg
     2821 agcctcaagc tgctgagcct ggaaaacacg caggtccgcg gccgccacct gggcaagttc
     2881 acgaatctgc agtcgcttga tctcgtctat tgcccgatca acgacgaggg cctgaagcag
     2941 atcggcggcc tcacgaagct tcgccagctc tacctccgcg acgccgtgat cagcgacgag
     3001 gggctggcca gcctcaagga cctcacgcgg ttggagcagc tcgacctggg cggcacccgc
     3061 atcggcgacg ccggcgcgac gcacctccgc gggatgacga agctcaagaa gctcagcttg
     3121 cagggcgcgg tcctgaccga tgcggggctg cgaaacctcg cggggctgac ggcgctagag
     3181 gagctgaacc tgtacggcac gaaggtgacc aactccggcg tcgatgtgct ggagaacatg
     3241 cagcgcctgc ggacgatcga cctgcgatac tcgcggctga cgcgggccgg cgtcgatcgc
     3301 ctgcgggccg ccgtgcccca gtgccaggtc acgtttctag gcgcttcggt gcggcccgcc
     3361 attcccgccg gagccgagcg gacagtgagc ggcgacgata gcgcggtcgc ccagtgggtg
     3421 cgggcgatcg gcggcaaagc cacgctcgac ggcggccggc tgatggcggt tgatctcgcg
     3481 acgaccagcg tgaccgacga cctgctccgc aatctgcgcg gcctggcgca cctgcgaaag
     3541 cttcagctcg gctcgaccga gatcggcgat cccggcctgg agtacctggc aggactaacc
     3601 accttgatgg agctcgacct gcgcggcacg gcgatttccg acgccggcct cgcgcacctg
     3661 gccggactga ccagcctccg ccagctcggc ctggccgaca cccagatcac gggcggcggc
     3721 ctgaagcatc tcgcgagcct gccgctcgaa gtgttgcggc tgtcgaacgc gcccgtcggc
     3781 gacgaaggcc ttgcccacct gggcaagatg gcctcgctgc gggagctcgc cctggccttc
     3841 accgacgtca ccaacgacgg cctggcgcag ctcgcgccgc tggccggtct ccagcggctc
     3901 gacctcgccg gaaccgacgt cggcgacaat ggtctggcac acgtgggcca attgacgggt
     3961 ctgacgggcc tggcactcga ctacacccgc atcaccgacg ctggagttcc gagactggag
     4021 aagctaacag gcctgaccca gctcggcctg acccgcaccc gagtgaccga caagagtatg
     4081 ggcacggtcg gccggctgac gcgcctcagc gagctcaagc tcgattacac cgacgtcggc
     4141 gacgagggtc tcgcggccct caaagatctg gcgcagctag cgcggctgag cctcgacagc
     4201 actaatgtga ccgacgccag cgccccgcgg ctggccgaat tcaagcagct gcgccagctg
     4261 aacctgtacc acacgtttta taccgacaag ggccgcgagc agctccgcgc cgccctgccg
     4321 cagtgcgagg tcatcttcga cgcccgctcg agcgatccca agcgccgccg cagctgatgg
     4381 agggctctaa tgacacccac ccgacgcgcc agcgagggag tgctgttcct ggctctgctg
     4441 accccaccac tctccctggc cgctgccgac caggccgtgc tggccgagtt ggacgcgctc
     4501 ggggctcgca ctacccgcaa cgaggccggc gaagtcacgg cggtcgatct taccaacgcc
     4561 tggctcagcg acgccgatct cgccc
//
LOCUS       DASRJB010000484         7785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_984077, whole genome shotgun sequence.
ACCESSION   DASRJB010000484 DASRJB010000000
VERSION     DASRJB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984077"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1771
                     /locus_tag="VFB80_12680"
     CDS             17..1771
                     /locus_tag="VFB80_12680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN34672.1"
                     /translation="MAADRWNETGRVWEDDHGLLDEEQVESCQRADVAEPTSPIPTRM
                     ISNGEYMPVPQTRQQKQVESRVGELAEKASRKLGISRRQFLAGSGGMAAALLAMNEVY
                     GKFFTVSEAELYEAAAYAEAAPPKDLFVFDDQLHMVRGSRPSPAALRAIAQGPTSAPR
                     VTQNPYNRSGQKDELGGEWSVWNPALVGLPIDPAYAHITQFIKDVFLDSQVTIGLLSN
                     VTASMVQVEGEPSRPPKNAEEAQRGEILTAAQTAAARNFINEISGSTRMLCHGLLYLG
                     KGNLAFIQEQTDKNEPDSWKGYNISNSAKIDDDPASLMRQWRHDDPEVAYPTFELIQK
                     NYEKLKARRPGFNNICVHKGLAPGPPDPKRGHPDDLPKAARDWPKLNFITYHSCIQPN
                     FFLADALEEVKAEKLRGGVPNISWTTEYCQLVGPLPNCYGEIGTTWASSVVTFPTIAA
                     HILGQLLKYLGEDRVVFGSDSVWYGSPQWQIEALWRFQIPAAMREKWGYPELTEQAKR
                     KILGLNSAKLYGIAGVDPAAYKPVPKDYESRMSEKLKSILEFGQLRADNLSRMKETYI
                     ALAIQPDLTRYGWIREPA"
     gene            1945..2883
                     /locus_tag="VFB80_12685"
     CDS             1945..2883
                     /locus_tag="VFB80_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34673.1"
                     /translation="MLTSLRTRAAFTLVELLVVIAIIGVLVALLLPAVQAARESARRS
                     SCTNNLRQLGLGTHLFHDVYLRFPAGTVWEPGAGGNQKGSEATWLTHLLPYVEQDNLF
                     KTGDFNQGFGQGNANHPNNRITSTFLKVTRCPSDVYPVQICSWGSSVAWARGNYVANN
                     GVGPMIETTTIDVNFRPQGVFMLQSKNRFADITDGTTSTVFLSELLNSPGNDWRGVMH
                     YPEGSLYHHNQTPNSRVPDEFRQGMCISILRAPCVPTSTSWNPKRITLTARSSHPGGV
                     IVVLGDAGTRWVSNNVTLATWKALSTPQGGETVGEF"
     gene            2886..3338
                     /locus_tag="VFB80_12690"
     CDS             2886..3338
                     /locus_tag="VFB80_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34674.1"
                     /translation="MNHHLRIHFALLATLALLCGCNGGKVEVRGMVKVDGQPLEEGSI
                     SLEPADGQGPTTGGMIQAGKYELVGEAAAVPGEKIVRIVGLRKTGKMVPAGPPAPKGT
                     LIAELVQCVPPNYNDKSSLRVDVVPGKANQHDFNLDSRAGAGGEAKGG"
     gene            complement(3394..5604)
                     /locus_tag="VFB80_12695"
     CDS             complement(3394..5604)
                     /locus_tag="VFB80_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34675.1"
                     /translation="MAHSNTLDELLARWDEAHSRGLELSPEELCAALPLADTAPAAPD
                     FLARLREQIRGLKEVDGLLRAQVRSSDPAPADVDLDGIMPITEQRYVIQSHLAKGGLG
                     TVFIAEDQELRREVALKFILERFANDPDCLASFRAEREITARMQHPGIVPVYGVGQLP
                     GGRPFYVMRYIKGRSLKEAIADFYARAAAMTRTQRELEFHKLLSSFVAACHTVAYAHN
                     RCVINRDLKPEHIMLGRYGETLVVDWGLAMRVAPPNAKSDPSANTVLADPTTSQSSRG
                     GAGTLVFMSPEQAAGAVFLTPATDIYSLGATLYVMLTGRLPFATKGTVHELRQKVIRG
                     EFVPPRQANPRCSKALDAVCRKAMSAEPEDRYATAEELAADVERYLADQPVSVLRDTR
                     SEALLRWTRRHRGAALTGLLSLVSVTILALVASLALLRSSHEAHAARQASLHTSATFA
                     AKMLGREVENRWLALELAAADPELLALLRRWELERDKTSGNSQLHRECDAWLAAQRRR
                     FDAALPAFSWVVNDQRGIQVGRQAADGKEFHGESFAHRTYFHGGQRELTTEQLRQARP
                     IARPNLSPVFENRSFADGSQQRHRLTVTCSVPIRDSGQVVGVLGMSLEITSFEALRLD
                     PVQQRIALLIDSRPDWKDDRGLVLFHGGQTARSRQPLLAQNDEWPVVDAAALEQMLKR
                     DAAGQIASGVLLDFHDPESRLAPQSNAGFAPVWVRPDRQQPHFSGWFVVVCEAR"
     gene            complement(5717..6583)
                     /locus_tag="VFB80_12700"
     CDS             complement(5717..6583)
                     /locus_tag="VFB80_12700"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN34676.1"
                     /translation="MHYLSTVSLLFLAAVCGADEQRIGLWNGRAPVGDGKFEEADAWI
                     TVHRPAKSGGTAVIICPGGGYGTLVKDAEGHGIAAWLNRHGITGVVLEYRLPAGRHLV
                     PLLDAQRAIRTTRSKAKDWEIDPARIGIMGFSAGGHLATSAGTHFDDGDSAAPDAASR
                     VSCRPDFLILVYPVVMMGEKTHRGCMNNLFGKDPSPKTVELFSNDKQVTADTPPAFLA
                     HALDDKVVPPEHSRAFYEALRAKKIAAEYLELPSGGHGLNGYRGPMWDAWQEKSLAWL
                     AALKTSRPPADR"
     gene            6746..>7785
                     /locus_tag="VFB80_12705"
     CDS             6746..>7785
                     /locus_tag="VFB80_12705"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34677.1"
                     /translation="MNCTSPRRLLLVAALTASLLGPAAAGAQNVAAEPAVRVDFGRQI
                     RPLLSDNCFKCHGPDAKAREAELRLDLREGALRSEKPVIVPGKSKESELWRRVSSTDP
                     DTVMPPPASGRKLTGDQKELVRQWIDGGAAWEQHWAFRAPMRPELPKFKLWYWPHTAI
                     DAFVAARLEPERLGPAPPASRETLIRRLSLDLTGLPPTPEEAAEFVADDSPDAWERLI
                     DRLLASPRFGERMVWEWLDAARYADTNGYQGDPTRTMWYWRDWAIGALNANQPFDQFT
                     VEQLAGDLLAAPTQSQLIATGFHRNHMINGEGGRIAEESRVDYVQDRVETTGTVWMGL
                     TFNCCRCHDHKF"
BASE COUNT         1353 a         2505 c         2698 g         1229 t
ORIGIN      
        1 gactcgggag gcaatcatgg ctgctgacag gtggaacgaa acggggcggg tgtgggagga
       61 cgatcatggg ctgctcgacg aggagcaggt ggagagctgc cagcgggcgg acgtggccga
      121 gccgacctcg ccgatcccga cgcggatgat cagcaacggc gagtacatgc cggtgccgca
      181 gacgcggcag cagaagcagg tcgagtcgcg agtgggggaa ttggcggaga aggccagccg
      241 gaagctggga atcagccggc ggcagtttct ggccggatcg ggcggaatgg cggcggccct
      301 gctggcgatg aacgaggtct atggaaaatt cttcaccgtc agcgaggcgg agctgtatga
      361 agccgcggcc tatgcggagg cagccccgcc gaaggatttg ttcgtcttcg acgaccagct
      421 gcacatggtc cgcggcagcc ggccgtcgcc ggcggccctg cgggcgatcg cccagggccc
      481 gacctccgcg ccgcgggtga cgcagaatcc gtacaaccgc agcgggcaga aggacgagtt
      541 gggcggcgag tggagcgtct ggaatccggc gctggtcggg ctgccgatcg atccggccta
      601 cgcccacatc acgcagttca tcaaggacgt ctttctggac agccaggtga cgatcggcct
      661 gctctcgaac gtgaccgcgt cgatggtgca ggtggagggc gagccgtcgc ggccgccgaa
      721 gaacgccgaa gaggcccagc ggggcgagat cctcaccgcc gcccagaccg ccgcggcccg
      781 caacttcatc aacgagattt cgggctcgac gcggatgctc tgccacggcc tgctctacct
      841 gggcaaaggc aacctggcgt tcatccagga gcagaccgac aagaacgaac cggattcgtg
      901 gaaaggctac aacatctcga actccgcgaa gatcgacgac gacccggcga gcctgatgcg
      961 gcaatggcgg cacgacgatc cggaggtggc ctatccgacg ttcgagctga ttcagaagaa
     1021 ctacgagaag ctgaaggcgc ggcggccggg cttcaacaac atctgcgtcc acaaggggct
     1081 cgcgccggga ccgcccgatc ccaagcgggg gcatcccgac gacctgccga aggcggcccg
     1141 cgattggccg aagctgaatt tcatcaccta ccactcctgc attcagccga acttcttcct
     1201 ggccgacgcc ctggaagagg tcaaggccga gaaactgcgc ggcggcgtgc cgaacatcag
     1261 ctggaccacg gagtattgcc agctggtcgg gccgctgccg aactgctacg gcgagatcgg
     1321 cacgacctgg gcctcgtccg tcgtgacgtt tccgacgatc gccgcccaca tcctcggcca
     1381 gctgctcaag tacctgggtg aggaccgcgt ggtcttcggc tccgactcgg tctggtacgg
     1441 ctcgccgcag tggcagatcg aagccctatg gcgattccag attccggccg ccatgcgtga
     1501 gaagtggggc taccccgagc tgaccgagca ggccaagcgc aaaatcctgg gactgaattc
     1561 cgcgaagctc tacggcatcg cgggagtgga tccggccgcc tacaagccgg tgcccaagga
     1621 ctacgagtcg cggatgagcg agaagctgaa atcgatcctg gaattcggcc agctgcgggc
     1681 cgacaacctt tccaggatga aggagacgta catcgcgctg gcgattcagc ccgacctgac
     1741 gcgctacggc tggattcgcg agccagcgta ggcgacgctg ccagcgtcgc aaaaactccg
     1801 acgctggcag cgtcggctac ggcgcttgac tggctgcgcc gcggcccctt acagtagcgc
     1861 gctggggggg aggcattcgc cgagttcctt tttgcgcgcg agaggaagag atggtgcaga
     1921 accggtaacg acaaggagcc tgccatgctc acgagcttgc gaacgagggc tgcgtttacg
     1981 ctggtcgagc tgttggtggt gatcgccatc atcggcgtgc tggtggctct gctcttgccc
     2041 gcagtacagg ctgcccgcga gtcggcccgc cgcagcagct gcacgaacaa cctgcggcag
     2101 ctgggcctgg gcacgcacct ctttcacgac gtttacctgc gatttcccgc gggcacggtc
     2161 tgggagcccg gggcaggcgg caatcaaaag ggttcggagg cgacctggct gacgcacctg
     2221 ctgccgtatg tcgagcagga caacctgttc aagacgggcg atttcaacca gggatttggc
     2281 cagggaaacg ccaatcatcc gaacaaccgg atcacgagta cgtttctgaa agtgacccgc
     2341 tgtccgtcgg acgtgtaccc ggtgcagatt tgctcctggg gcagttcggt ggcctgggcc
     2401 cgcggcaatt acgtcgccaa caacggtgtc gggccgatga tcgagacgac gacgatcgac
     2461 gtcaacttcc gaccgcaggg cgtgttcatg ctgcaaagca agaaccgctt cgccgacatc
     2521 accgacggga cgacgagcac cgtgtttctc tccgagctgc tgaacagccc gggcaacgac
     2581 tggcgcggcg tcatgcacta tcccgagggc tcgctctatc accacaacca gacgcccaac
     2641 agccgcgtgc ccgatgagtt ccgccaaggc atgtgtatct cgattctgcg cgcgccgtgc
     2701 gtgccgacct cgacgagctg gaaccccaag cgaatcacgc tgaccgcccg cagcagccat
     2761 cccggcggcg tgatcgtcgt cctcggcgac gccggcacgc ggtgggtctc gaacaacgtc
     2821 acgctcgcca catggaaggc gctctcaacg ccgcagggcg gcgagacggt gggcgagttc
     2881 tgacgatgaa ccatcacctg cgaatccact ttgccctgct ggccaccctg gccctgctgt
     2941 gcggatgcaa cggcggcaag gtcgaagtgc gcggcatggt gaaggtcgat ggccagccgc
     3001 tcgaggaggg ctcgatcagc ctggagcccg ccgatggcca ggggccaacg acgggcggca
     3061 tgatccaggc cggcaagtac gagctggtcg gcgaggcggc cgccgttccc ggcgagaaga
     3121 tcgtccgcat cgtcggcctg cgaaagaccg gcaagatggt gccggccggg ccgcccgcgc
     3181 ccaagggcac gctgattgcc gagctcgtgc aatgcgtgcc gccgaactac aacgacaaaa
     3241 gcagcctccg cgtcgatgtg gttcccggca aggccaacca gcacgacttc aacctcgact
     3301 cgcgcgctgg cgccggcggc gaggccaagg gaggatgagc ctggccgccg cagcgcacgc
     3361 cgcgctgccg gcctgggggc cggggctaag cggctagcgt gcctcgcaga cgacgacgaa
     3421 ccagccgctg aagtgcggct gctggcggtc gggccggacc cagacgggtg cgaagccggc
     3481 gttcgactgg ggggcgaggc ggctttccgg atcgtggaag tcgagtagca cgccgctggc
     3541 gatctggccg gcggcgtcgc gcttcagcat ctgttccagg gcggccgcat ccaccacggg
     3601 ccactcgtcg ttctgcgcta acagcggctg acgcgagcgg gcagtctggc cgccgtgaaa
     3661 caggaccagt ccgcggtcgt ccttccagtc cggccgcgag tcgatcagca gggcgattcg
     3721 ctgctggacg ggatcgagcc gcagggcctc gaacgaagtg atctccagcg acatgccgag
     3781 gacgccgacg acctggccgc tgtcgcggat gggcaccgag caggtcaccg ttagccggtg
     3841 ccgctgctgc gatccgtcgg cgaatgagcg gttttcgaag acgggcgaga ggttcggcct
     3901 ggcgatcggc cgcgcctgcc ggagctgctc cgtcgtcagc tcgcgctggc cgccgtgaaa
     3961 gtaagtgcga tgggcgaacg attcgccgtg gaactccttg ccgtcggcgg cctggcggcc
     4021 gacctggatg ccgcgctgat cgttgaccac ccagctgaag gccgggaggg cggcgtcgaa
     4081 ccgccgccgc tgggcggcca gccaggcgtc gcattcccgg tggagctggc tgttgccgga
     4141 cgtcttgtcg cgctccagtt cccagcggcg gagcagcgcg agcagttccg gatcggcggc
     4201 ggccagttcc agcgccagcc agcggttttc cacttcgcgg ccgagcatct tggcggcgaa
     4261 cgtggccgag gtgtgcaggc tggcctgccg ggcagcgtgt gcttcgtgac tggaccgcag
     4321 cagcgccagc gatgcgacga gcgccaggat cgtcacggag acgagcgaca ggagcccggt
     4381 cagtgccgcg ccgcggtgcc ggcgcgtcca gcgcagcagc gcttcgctcc gcgtatcgcg
     4441 gagcaccgac accggctgat cggccaggta ccgctcgacg tcggccgcca gctcctccgc
     4501 cgtggcgtag cggtcttccg gttcggccga catcgccttg cggcagacgg cgtcgagcgc
     4561 cttggagcag cgcggattgg cctgccgcgg cggcacaaat tcgccgcgga tgaccttctg
     4621 gcgcagctcg tgaacggtgc ccttggtcgc aaacggcaat cggccggtga gcatgacgta
     4681 cagcgtcgcc cccaggctgt agatgtcggt cgcgggcgtg aggaacaccg cgccggccgc
     4741 ctgctccggg ctcatgaata ccagcgtgcc cgcgccgccg cgggaggact ggctggtggt
     4801 cgggtcggcc agcacggtgt tggcgctggg gtcgctcttg gcattgggcg gagcgacgcg
     4861 catcgccagt ccccaatcga caacgagcgt ctcgccgtag cggcccagca tgatgtgctc
     4921 gggcttgagg tctcggttga tcacgcagcg attgtgggcg taggccaccg tgtggcaggc
     4981 ggcgacgaag ctggacagca gcttgtggaa ttcgagctcc cgctgggtcc gcgtcatggc
     5041 ggccgcgcgg gcgtagaaat cggcgatcgc ctccttgagc gagcggccct tgatgtaacg
     5101 catcacgtaa aacggccggc cgcccggcag ctggccgacg ccgtaaaccg gcacgatgcc
     5161 cggatgctgc atgcgggcgg tgatttcgcg ttccgcgcga aagctggcca ggcagtccgg
     5221 gtcgttggca aaccgctcca ggatgaattt gagcgcgacc tcgcggcgca gctcctggtc
     5281 ctcggcgatg aacacggtgc ccaggccgcc cttggccagg tggctctgaa tcacgtaccg
     5341 ctgctcggtg atcggcatga ttccgtccag gtccacgtcg gcgggagccg gatcgctgga
     5401 gcggacctgc gcccgcaaga gcccgtccac ttccttgagc ccgcgaatct gctcgcgcag
     5461 gcgggccagg aaatcgggcg cggccggcgc agtgtccgct agcggcagcg ccgcacacag
     5521 ctcctccggg gacagctcca ggccccggct gtgggcctcg tcccagcggg cgagcagctc
     5581 gtcgagggtg ttgctgtggg ccatgacagg gccgattggt gaaaagggcg cagtccctga
     5641 acaggaaagc gcaaactaca gggccgatgt gacacgaatt ctttcaaacg tggcggatcg
     5701 gccagaatcc cgcgatctag cgatcagcgg gaggccggct ggttttgagc gcggcgagcc
     5761 aggccagcga cttttcttgc caggcgtccc acatcggccc gcgatagccg ttcaggccgt
     5821 gaccgcccga cggcaattcg agatactccg ccgcaatctt cttggcgcgc agagcctcgt
     5881 agaatgcccg gctgtgctcg ggcggcacga ctttgtcgtc gagggcatga gccagaaacg
     5941 ccggcggcgt gtcggctgtc acctgcttgt cgttcgagaa gagctcgacg gtcttgggcg
     6001 acggatcttt gccgaacagg ttgttcatgc agccgcggtg ggtcttttcg cccatcatga
     6061 cgaccggata cacgaggatc agaaagtcgg ggcgacaact gacgcggcta gcggcgtcgg
     6121 gcgctgcaga gtcgccgtca tcgaagtgcg tgcctgccga ggtcgccaga tgtccgccgg
     6181 ccgagaagcc catgatgccg atccgcgccg gatcgatctc ccagtccttc gccttggaac
     6241 gcgtcgtgcg gattgctcgc tgtgcgtcga gcagcggcac gaggtgccgg ccggcgggaa
     6301 ggcggtattc gaggacgacg cccgtgatgc cgtggcggtt cagccacgcg gcgatgccgt
     6361 ggccctcggc atccttgacg agcgtgccat aacccccgcc ggggcaaatg atgacggccg
     6421 taccgccgga tttcgcgggt cggtgtaccg tgatccaggc gtccgcttcc tcgaacttgc
     6481 cgtcgcccac gggcgcgcgg ccgttccaca ggccgatccg ctgctcgtca gcgccgcaga
     6541 ccgctgccag aaacagaagg gagactgtgg agaggtagtg catagttcga tgtctctatc
     6601 tcgagtcggt cggcgaaggg actgcattct tgccgaaagt aacgggcgaa atcaatcagc
     6661 gatcaaaacc ggttgtttgc gatcggccat taggtttgaa tggagaacta gcccgcctcc
     6721 accgcccccg aaagttgtcg cctgcatgaa ttgcacctcg ccgcgacgct tgctgctcgt
     6781 ggccgctttg accgcctcct tgctcggccc cgcggccgct ggtgctcaaa acgttgccgc
     6841 cgagccagcc gtgcgcgtcg attttggccg gcagattcgc ccgctgttgt cggacaattg
     6901 tttcaagtgt catggaccgg acgccaaggc ccgcgaagcc gaattgcggc tggacctgcg
     6961 cgagggcgca ctgcgcagcg agaagcccgt gatcgtgccg ggcaagagca aggaaagcga
     7021 gttgtggcgc cgcgtctcct cgaccgatcc ggacacggtg atgccgccgc cggccagcgg
     7081 ccgcaagctg accggcgacc agaaggagct ggttcgccag tggatcgacg gcggcgcggc
     7141 gtgggagcag cactgggcgt ttcgagcgcc gatgcgtccc gagctgccca agttcaagct
     7201 ctggtactgg ccgcacacgg cgatcgacgc gttcgttgca gcccgcctgg agcccgaacg
     7261 gctcgggccc gctccgccgg ccagccgcga aacgctcatc cgccgcctgt cgctcgacct
     7321 gaccgggttg ccgcccacgc ccgaagaggc ggcggagttt gtcgctgacg actcgcccga
     7381 cgcctgggag cggctgatcg accggctgct ggcgtcgcca cggtttggcg agcggatggt
     7441 ctgggaatgg ctcgacgccg cccgctacgc cgacaccaac ggctaccagg gggatccgac
     7501 ccggacgatg tggtactggc gcgactgggc gatcggcgcg ctgaacgcca atcagccgtt
     7561 cgatcagttc acggtcgagc agctcgcggg cgacctgctg gctgcgccga cgcagtcgca
     7621 gctcatcgcg accggctttc accgcaatca catgatcaac ggcgaaggcg ggcggatcgc
     7681 ggaggagtca cgggtcgatt acgtgcagga ccgcgtcgag acgacgggca cggtctggat
     7741 ggggctaacg ttcaattgct gccgctgcca cgaccacaag ttcga
//
LOCUS       DASRJB010000485         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_696925, whole genome shotgun sequence.
ACCESSION   DASRJB010000485 DASRJB010000000
VERSION     DASRJB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696925"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFB80_12710"
     CDS             complement(<1..214)
                     /locus_tag="VFB80_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34678.1"
                     /translation="MSTAVFDPYEAWLGIAPAEQPADHYRLLGLARFENDASRIARAA
                     DERMALVRSYQVGPRGAYTQRLLNELS"
     gene            complement(276..2777)
                     /locus_tag="VFB80_12715"
     CDS             complement(276..2777)
                     /locus_tag="VFB80_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN34679.1"
                     /translation="MQQPPTRDQLASLYLDQLPFPPYPVQEEALLAYFTAEQGVLVCA
                     PTGTGKTLIAEAALFEALHTGTRAYYTTPLIALTEQKLQEMQAAAVRWGFSADDIGLV
                     TGNRRVNPDAPVLVVVAEILFNRLLHGAREGGKEGRSDRETERQSNPSVTPSLLPSIP
                     SPLGDRSALNPALDFSNVSAVVMDEFHNFADPERGIVWELALGLLPAHVRLLLLSATV
                     GNAFEFRQWLQAAHHRSIELVQSSERKVPLQYQWVGDQLLSEQLEKMAEGDEAKRYTP
                     ALVFCFNRDECWNVAEMLKGKSLLADGQQARLADALARYDFSEGAGPKLKAILQRGVG
                     VHHAGVLPKYRRIVEELFQQKLLSVCVCTETLAAGINLPARSVVLPTLLKGKPGEMKL
                     IDSSTAHQIFGRAGRPQFDAQGYVFALAHEDDVKILRAKEKLDKIPEDTKDVGLLRMR
                     KELKRKLPTRRATEQYWSEPQWDKLRTAPPLKLASRGPIPWRLLAYMLEASPEVEPIR
                     RLAGKRLMESGRMVGAQKQLDQMLRTLWAGGYVRLEPTPPADGETEGKSDGGKERQKE
                     EPRRKPLLDVTVRAPVSPSLPPSVAPSPPPAPPVYQAKFAHSTEDLPKLLRLRGINPL
                     YGVFLVNQLGIASREERLQAFESVLEMPGSVAKHVRVPKYEEMPPGPLATTRLDVELL
                     QMGLATPEQLGAKPAQEEDEPQQRRGMFEEKVWPLTLGEKLRLLFDATFSEVHSLFTT
                     SVWAAGEVLEFGGNFNKYITSRGLQKQEGVIFRHLLRLILLVKEFQQFTPPDCSPEEW
                     LAELRDIADRLADCCRRVDPQSTDKTLEEAEREAF"
     gene            2880..3362
                     /locus_tag="VFB80_12720"
     CDS             2880..3362
                     /locus_tag="VFB80_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34680.1"
                     /translation="MSLAIAETARWVGGTPDSSASIRQQTNHNVVRLASGEPGAIDRR
                     LRELHDEWALERTLEANAAAAALVGLGLGAFVDRRFFLLPALAAGLLLQHAASGWCPP
                     LTWFRRLGLRTHSEIDEERYALKALRGDFRHVAAAGESGRPEQAQAALRAVRRAWRWK
                     "
     gene            3369..4262
                     /locus_tag="VFB80_12725"
     CDS             3369..4262
                     /locus_tag="VFB80_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZN34681.1"
                     /translation="MWKTLSETVNEFLQDECPRLAAALAYYAIFSLPALLVVVISIAG
                     LVVNKQAATDRLIAHVSEAMGPRTAENLQTILDQAHLPGHGLWGSLVGAAVLLVGASG
                     VLLELQTALNRAWGVQPDPKQSGWRAFVLKRVLSLGMLLGVAFLLLTSLVASWLLAEF
                     GQWIDAHAPAGLSSRVIWALNAALSLSIITLLFAAILKFLPDAKIAWSDVWAGALATS
                     VLFVLGKFGLGLYFAWADPTSAFGAAGSLALLLLWIYYSAMILFFGAEFTQVLARQHG
                     KRVVPEAGAAHLPAASSKATA"
     gene            complement(4259..4681)
                     /locus_tag="VFB80_12730"
     CDS             complement(4259..4681)
                     /locus_tag="VFB80_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34682.1"
                     /translation="MVSRLSWMTIVAVFAAGCGQPSGTVSGTVTFHNVPIEQGLITFF
                     PLGAEGGSAGGEIVKGKYTVADLKPAKYHVVVEATKEPKFTTPNDPANSRTKSAEEIR
                     AQYDPLPLDTSGKEQDLEVKGGPQTLDFNLASKSKPKL"
     gene            4698..>4933
                     /locus_tag="VFB80_12735"
     CDS             4698..>4933
                     /locus_tag="VFB80_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34683.1"
                     /translation="MREKQAAARVGEIFRQSRRTINWKRKNLNRNQADGTLQPPGGRR
                     FVSIPENPMTMPAQWRFRKSPAPPEWRGFTLVELL"
BASE COUNT          769 a         1622 c         1617 g          925 t
ORIGIN      
        1 ccgacaattc attgagcagc ctctgcgtat aggcgccccg cgggcccacc tggtagctcc
       61 gcaccagcgc catccgctcg tctgcggccc gtgcaatccg cgaggcatcg ttctcgaatc
      121 gcgccagccc cagcagccga taatggtccg ccggctgctc cgctggcgcg atgccgagcc
      181 aggcctcgta ggggtcgaag acggcagtgc tcaagacggg gtcgggggtc gggagtcggg
      241 agtcagtaag aaagcgtagt atctgttccg gcccattaga acgcttctcg ctcggcttcc
      301 tccagcgtct tgtccgtgct ttgcggatcg acgcggcggc agcagtcggc caggcggtcg
      361 gcgatgtcgc gcagctccgc cagccactcc tccggcgaac agtcgggcgg cgtgaactgc
      421 tggaattcct tgaccagcag aatcagcctg agcaggtgcc ggaagatcac cccttcctgc
      481 ttctggaggc cgcggctggt gatgtacttg ttgaagttgc cgccgaactc gagcacctcg
      541 cccgcagccc agacggaggt cgtgaagagc gaatggacct cggaaaacgt cgcgtcgaag
      601 agcagccgca gcttttcgcc gagcgtgagc ggccagactt tttcctcgaa catgccgcgg
      661 cgctgctgcg gctcgtcctc ttcctgggcc ggtttcgctc ccagttgctc gggcgtcgcc
      721 aggcccattt gcagcagctc cacgtccagt cgcgttgtgg ccagcggccc cggcggcatc
      781 tcctcgtact tcggtacgcg gacgtgcttg gctaccgagc cgggcatttc caggacgctc
      841 tcaaaggcct gcagccgttc ctcgcggctg gcgatgccga gctgattcac caggaacacg
      901 ccgtacagcg gattgatgcc gcggagtctt agaagcttgg ggaggtcttc ggttgagtgg
      961 gcgaatttgg cctgatagac cggaggcgct ggaggcggcg agggagcgac ggagggaggg
     1021 agggacggag agacgggggc acggaccgtt acgtcgagca acggcttgcg gcgcggctct
     1081 tccttctgtc gctccttccc tccatcactc tttccctccg tctctccgtc cgccggcggc
     1141 gtcggctcca gccgcacata cccgccggcc cacagcgtcc gcagcatctg atcgagctgc
     1201 ttctgcgcgc cgaccatccg gccactttcc atcaggcgct tgccggccag ccggcggatc
     1261 ggttcgactt ccggcgatgc ctcgagcatg taggccaaga gccgccaggg aatcggcccg
     1321 cggctggcga gcttgagggg cggcgcggtg cggagcttgt cccattgcgg ctcggaccag
     1381 tactgctcgg tggcccgccg cgtcggcagc ttccgcttca gctccttccg catccgcagc
     1441 aggccgacgt ctttcgtgtc ctcgggaatc ttgtcgagct tctctttggc ccgcaggatc
     1501 ttcacatcgt cttcgtgggc cagggcgaag acgtagccct gggcgtcgaa ctgcggccgg
     1561 ccggcgcggc cgaaaatctg gtgggccgtg ctggagtcga tcaatttcat ctcgcccggt
     1621 ttgcccttga gcagcgtggg cagcacaacg ctgcgggccg gcaggttgat ccccgccgcc
     1681 agcgtctcgg tgcagacgca gaccgagagc agcttctgct gaaacaactc ctcgacgatg
     1741 cggcggtatt tcggcagcac gccggcgtgg tgcacgccta cgccgcgctg caagatcgcc
     1801 ttcagcttcg gcccggcgcc ctcgctgaag tcgtatcggg ccaatgcatc ggccaggcgc
     1861 gcctgctgcc cgtcggctag cagcgacttg cccttgagca tctcggccac gttccagcac
     1921 tcgtcgcgat tgaagcaaaa gaccagcgcc ggcgtgtacc gcttcgcctc gtcgccctcg
     1981 gccattttct ccagctgctc cgagagcagt tgatcgccga cccactggta ttgcagcggc
     2041 accttccgct cgctgctctg caccagctcg atagaacggt ggtgcgccgc ttgcagccac
     2101 tggcggaact cgaacgcgtt gcccaccgtc gccgacaaga gcagcagccg cacgtgcgcc
     2161 ggcagcaggc ccagggccag ttcccacacg attccccgct ccggatcggc gaagttatgg
     2221 aactcgtcca tcaccaccgc tgagacgttg gaaaaatcga gtgcgggatt gagcgcggaa
     2281 cggtctccca atggagacgg gatggaggga aggagcgaag gagtgacgga gggattgctc
     2341 tgtctctcgg tctctctgtc gctccttccc tccttcccgc cctcccgcgc cccatgcagg
     2401 aggcggttaa acagaatctc ggccaccacc accagcaccg gcgcgtcggg attgacgcgg
     2461 cggttgccgg tgaccaggcc gatgtcgtcg gccgagaagc cccagcgaac agcggcggcc
     2521 tgcatctcct ggagcttctg ctcggtgagc gcaattagcg gcgtcgtgta ataggcccgc
     2581 gtgccggtgt gcagcgcctc gaagagggcc gcctcggcga tcagcgtctt gccggtgccc
     2641 gtcggggcgc agaccagcac gccctgttcg gccgtaaaat aggccagcag cgcttcttcc
     2701 tggaccgggt agggcggaaa cggcagctga tcgagataga gcgaggcgag ctgatcgcgc
     2761 gtcggcggct gttgcatgct tcgcattctc gccgcccgac cgccggcctc caaggcggcg
     2821 actggcccgt ggtttgcaca ctctggctct gttacagtct cgaacgaacg agggaaacga
     2881 tgtctttggc gatcgcggaa accgcccgat gggttggcgg cacgccggat tcgagcgcgt
     2941 caatccggca gcagacgaat cacaacgtcg tccgcctggc cagcggcgag ccgggggcca
     3001 tcgaccgccg cctgcgggag ctccacgacg agtgggccct ggagcggacg ctcgaggcca
     3061 acgcggccgc cgcggcgctg gtcggcttgg ggctgggggc cttcgtcgat cggcgcttct
     3121 ttttgctgcc cgcgctcgct gccgggcttt tgctgcagca cgcggccagc ggctggtgcc
     3181 cgccgctgac ctggttccgg cggctgggcc tgcgcacgca ctcggagatt gacgaagaac
     3241 ggtacgccct gaaggcgcta cgcggtgatt ttcggcacgt cgcggcggcc ggcgaatcag
     3301 gccgcccgga gcaagcgcag gcggcgctgc gggccgtgcg gcgcgcgtgg cgctggaaat
     3361 gacgatccat gtggaagact ctctccgaaa cggtgaatga gttcctgcag gacgagtgtc
     3421 cgcggctggc ggcggcgctg gcgtactacg ctatcttctc gctgccagcg ctgctggtcg
     3481 tcgtgatctc gatcgccggg ctggtggtca acaagcaggc tgcgaccgac cgcttaatcg
     3541 cccacgtttc ggaggcaatg ggtccgcgga cggccgagaa tctgcagacg atactggacc
     3601 aggcccatct gccgggccac ggcctgtggg gctccctcgt cggcgcggcg gtcttgctgg
     3661 tcggggccag cggcgtcttg ctggaactgc aaaccgcgct gaaccgggcc tggggcgtcc
     3721 agcccgatcc caagcaaagc ggctggcggg cgttcgtgct caagcgcgtg ctctcgctgg
     3781 ggatgctcct gggcgtggcg tttctgctgc tgacgtcgct cgtggccagc tggctgttgg
     3841 cggagttcgg ccagtggatc gacgcgcacg ctccggccgg actttcgagc cgcgtcatct
     3901 gggccttgaa cgcggcgctg tcgctgtcga tcatcacgct gctcttcgcg gcgattctca
     3961 agttcctgcc cgacgccaag atcgcctggt ccgacgtctg ggccggcgcg ctggccacgt
     4021 ccgtgctgtt cgtgctgggc aagttcggcc tgggcctgta cttcgcctgg gccgatccga
     4081 ccagcgcctt cggcgccgcc ggctcgctgg ctctcttgct gctctggatc tattactcgg
     4141 cgatgatcct gttcttcggg gcggagttta cgcaagtgct cgcccgccag cacggcaagc
     4201 gcgtcgtacc cgaagccggc gcggcccatt tacccgctgc atcgagcaaa gcaacggcct
     4261 agagcttggg cttggatttc gacgccagat tgaaatcgag cgtctgcggt ccacccttta
     4321 cctccaggtc ctgctccttg cccgatgtgt ccagcggcag cgggtcgtat tgggcgcgaa
     4381 tttcctcggc ggactttgtg cggctgttgg ccggatcgtt cggagtggta aatttgggct
     4441 ccttggttgc ctcaacgacg acgtggtact tggccggctt caggtcagca accgtgtatt
     4501 tccccttgac aatctcgccg cccgccgagc cgccttctgc ccccagggga aagaacgtaa
     4561 tcaggccctg ctcaatcggc acgttgtgaa acgtgaccgt cccgctcacg gtgcccgaag
     4621 gctggccgca gccagcagcg aagaccgcca cgatcgtcat ccaactcagt cgggaaacca
     4681 tattggactc gcatcttatg cgggagaagc aggcagcagc gcgcgttggt gagatttttc
     4741 gccaaagcag gcggacaatc aactggaaac gcaaaaactt gaatcgaaat caagccgatg
     4801 gtactttaca gccgcctggg ggacgccgct ttgtttcgat tccggagaac cccatgacta
     4861 tgcctgcgca gtggcgcttc aggaaatctc ccgctccgcc tgaatggcgc ggtttcacgc
     4921 tcgtcgagct cct
//
LOCUS       DASRJB010000486         4565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_985236, whole genome shotgun sequence.
ACCESSION   DASRJB010000486 DASRJB010000000
VERSION     DASRJB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985236"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFB80_12740"
     CDS             complement(<1..839)
                     /locus_tag="VFB80_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34684.1"
                     /translation="MDSTTSNFGKTWITPDRLLLGGVLLAALIVRGGALWALRGNLDG
                     DPDAYRQIAENLLRHGVFAMGDSDDPPPPTAFRPPLYPIVLSNLPDASGHNVSLGKVA
                     VLHVLLGVATVWLTWLVARRVGSARASSAGPPLAGQASDGGPAAADHRSAAEPALSHP
                     TIAALLVACDPILLNQQSLVMTETLATFLAVLALWCLARFSERPSWFWAGLSGGAIGL
                     AALCRPTFLPWLGLVGIAMVVIQNSDFRIQKCSAQARSWWAGLGVRLARALALVAVAA
                     TVMS"
     gene            1077..1475
                     /locus_tag="VFB80_12745"
     CDS             1077..1475
                     /locus_tag="VFB80_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN34685.1"
                     /translation="MVLITPRLLITDDDRDFRETVAGLLADRGFETLQAGDGEEALEV
                     VARHEVHLLLLDMHMPRLSGLEMIERLHARELLVPLPWILISAGLDDAIVARARAAAA
                     YSVLPKPVRLPELTGAVSKALADAYGWRAA"
     gene            1856..2797
                     /locus_tag="VFB80_12750"
     CDS             1856..2797
                     /locus_tag="VFB80_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34686.1"
                     /translation="MGTAVLAVGLLSVAVRAAEPAAGTAYRVLAQDQGRVAIIDAHGG
                     VEWEVACKHNSHDIACLANGNFLLHTGPAKIDELTPGKQLVWTYTARPRPGYSGRVEI
                     HAFQRLPDGNTLVAESGNRRLVEVDRDGKLAREIPLTVEHPDPHRDTRLARKLASGNY
                     LVCHEADGAVREYDPAGKVVWTYRLDLAGRPASPGHGPEGHGTSVFGALRVASGNTLI
                     ACGNGNRVIEVTPEGKTVWSIEQKELPGITLAWVTTLQVLKNGNIIFGNCHAGEGNPQ
                     LIEVTKDKKVVWTFRDFTHFGNSLASAQILGPEDVLR"
     gene            complement(2830..3750)
                     /locus_tag="VFB80_12755"
     CDS             complement(2830..3750)
                     /locus_tag="VFB80_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZN34687.1"
                     /translation="MTILLLLTIYCSLIVVASLLGGALPGWFRLTHRQMQLVMSFVGG
                     LMLGVAVLHLLPHSVAEQGSVDRAAAWTLGGLLTMFLLIRVFHVHAHEHGDAGDVLDE
                     HRHTHDHEGPCSHEHQHQHAHGQSRGTHAFSWLGLAFGLSLHTLIDGLALGAAVAADA
                     HEARSFALYGLATFLAVALHKPLDALSITSLMAAGGWKQSDIQLANAIFALMCPLGAL
                     AFVFGFAGLFAEQHVVVGCALAFAAGVFLCISLADLLPEVAFHTHDRVRLTLALMAGI
                     ALAWLIGFIEPGHSHQHHHDTAHQHSENGP"
     gene            3889..>4565
                     /locus_tag="VFB80_12760"
     CDS             3889..>4565
                     /locus_tag="VFB80_12760"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34688.1"
                     /translation="MKVTVLANLENGKSEPDAVVPQITRALLARGDKTNVVATFDDVE
                     QLVRDLKAAKTDLVFNVFESFGHEVIGGQMGVTGILDLLQIPYTGGGPGELYLQEDKA
                     LAKKLLAYERIQYPDFAIFAPNADFETGGKLRMPLFVKPLRMDASIGIDEKSLVRNMQ
                     QLMDRVMKIHKELGDAALVEEFIEGREFYVGVLGNHDLLAFPPIEMDFSGMPKGSLKV
                     MDAKAKF"
BASE COUNT          868 a         1464 c         1479 g          754 t
ORIGIN      
        1 gacatcaccg tggccgcaac tgccaccagt gccagcgctc tggccaagcg gacgccgagc
       61 cccgcccacc aagacctcgc ctgagcgctg catttctgaa ttctgaaatc tgaattctga
      121 attacgacca tcgcgatccc gaccagcccc agccagggca ggaacgtcgg ccggcaaagc
      181 gcggccaggc cgatcgctcc gccggacaag ccggcccaga accaggaggg acgctcgctg
      241 aaccgcgcaa ggcaccaaag agccagcacc gccaaaaacg tcgccagcgt ctcggtcatc
      301 accagcgact gctggttcag gaggatcggg tcgcaggcaa cgagtagtgc cgcgattgta
      361 gggtgggaca aggcgggctc cgccgcggag cggtggtcgg cggccgccgg cccaccatcg
      421 ctcgcctgcc cggctaatgg tgggccggcg ctcgatgctc tcgctgatcc caccctacgg
      481 gcgacgagcc acgtcagcca cacagtcgcc acgcccagca gcacgtgcag cacggcgacc
      541 ttgcccagcg agacgttgtg gccgctggcg tcgggcaggt tgctcagcac gatcggatag
      601 agcggcggcc ggaaagccgt cggcggtggc gggtcgtcgc tgtcccccat tgcaaagacg
      661 ccgtgccgca gcaggttctc ggcgatctgg cgataggcgt cggggtcgcc gtccagattg
      721 ccccgcagcg cccagagcgc tcctccgcgg acgatcagcg ccgccagcag cacacccccc
      781 agcagcagcc ggtcgggggt gatccaggtc ttgccgaaat tgcttgtggt tgagtccatg
      841 ccttgcaaat tggcagccgc cgcagccggc cgccaagaat ttgactttct tcagtttaca
      901 acgtttgata gaagtacagc gccggtaacg ggttgcggca gcacgccagg ccgggttgcg
      961 gcagcacggt agtcattgag cagtacccgg catcctgctt gcattgacgt aggaattagc
     1021 tgttccgctg tcagtttggt tcttgggttc ttcggttctt tgatttttcg attgccgtgg
     1081 ttctgattac gccccgcctg ctgatcaccg acgacgatcg cgactttcgc gagacggtgg
     1141 ccgggctgtt ggccgatcgc ggcttcgaga cgctgcaggc cggcgacggc gaagaagccc
     1201 tggaggtcgt cgcccgccac gaggtgcatc tgctgctgct cgacatgcac atgccgcggc
     1261 taagcggcct ggaaatgatc gagcggctgc acgcccgcga gctcttggtg ccgctgccat
     1321 ggatcctgat ctcggcgggc cttgacgacg ccattgtcgc ccgggcccgg gcggctgcgg
     1381 cctattcggt cttgcccaag cccgtgcggc tgccggagtt gaccggtgcg gtgagcaagg
     1441 cgctggccga cgcctacggc tggcgcgcgg catagcggtt cttcgaccag ggacgggaac
     1501 attggcgttt tttcagcggc gatggaagtt tctaccatcg cctgtgggca ggccgcgcga
     1561 agccagcacc gctggcagtc cttaaaaccg ggtcgactcg cacgacccgg ccgctagcgg
     1621 ctgcggcgcg cccactttcg cgactttccg tgcccgcggc acaatacgtg ctttgccagt
     1681 ccgtgccggt caggatgccc ggcaaaagca actttcaggt cgaagggccc cttggggcag
     1741 gttgcggccg cacggacttg ctgctgcaaa cgtctgcttg catcgccctt ccctcgctga
     1801 aaggtgcacc atgggtatcc tcggtcgtag gttagcaaac tccagttgga cggccatggg
     1861 aaccgcggta ctggcggtcg ggctcctaag cgtggcggtt cgcgccgcgg agcccgccgc
     1921 cgggaccgcc taccgagtgc tggcccagga tcagggccgc gtggccatta tcgacgcaca
     1981 cggaggtgtg gaatgggagg tcgcctgcaa gcacaactcg cacgacatcg cgtgtttggc
     2041 aaatggcaac ttcctcctcc atacgggccc cgcaaagatc gacgagctga cgccaggcaa
     2101 acaactcgtc tggacctata ccgcccgacc ccggcccgga tactccggac gagtcgagat
     2161 tcatgccttc cagcggctgc ctgacggcaa cacgctcgtc gccgaaagcg gcaaccgccg
     2221 cctcgtcgaa gtcgatcgcg acggcaagct tgcccgcgaa atcccgctca ccgtcgagca
     2281 tcccgatccg caccgcgaca cgcgcttggc ccgcaagctc gccagcggca actacctggt
     2341 ctgccacgaa gccgacgggg ccgtccgcga gtacgacccc gcgggcaaag tcgtctggac
     2401 ctaccggctc gatctcgccg gccgccccgc ttcgcccggc cacggccccg aaggccacgg
     2461 cacgtccgtc ttcggcgcgc tgcgggtggc cagcggcaac acgctcatcg cctgcggcaa
     2521 cggcaaccgc gtgatcgaag tcacgcccga gggcaaaacc gtctggagca tcgagcaaaa
     2581 ggagctgccc ggtatcacgc tggcctgggt caccacgctg caagtgctca aaaatgggaa
     2641 catcatcttc ggcaactgcc acgccggcga gggcaacccg cagctcatcg aggtcaccaa
     2701 ggacaagaag gtcgtctgga ccttccgcga cttcacccac ttcggcaaca gcctcgcctc
     2761 ggcgcaaatc ttaggccccg aggacgtcct gcggtgagac actaagcatg aggcgggacg
     2821 aagttgcgac taggggccgt tctcggagtg ctgatgtgct gtgtcgtgat ggtgctggtg
     2881 ggaatgccct ggctcgatga agccgatcaa ccaggccagc gcaatgcccg ccatcagcgc
     2941 cagcgtcagg cgcacgcggt cgtgtgtgtg aaaggcaact tcgggcaaca gatcggcgag
     3001 cgagatgcag aggaagacgc ccgcggcaaa ggccagggcg caaccgacga cgacgtgctg
     3061 ctcggcaaac aatccggcga agccgaacac gaaagccagc gcacccagcg ggcacatcag
     3121 cgcaaaaatg gcgttggcca gctggatgtc gctctgcttc cagccgcccg ccgccatcag
     3181 cgaggtgatc gagagggcgt caagcggctt atgcagagcg accgccagaa aggtggccag
     3241 gccgtacaga gcgaagctgc gcgcctcatg tgcatcggcc gcgacggcag cgccgagcgc
     3301 gaggccgtcg atcaacgtat gcagcgatag accgaaagcc agtcccagcc agctaaaggc
     3361 gtgagtgccg cggctttgcc cgtgggcgtg ctggtgctga tgttcgtgcg agcaaggtcc
     3421 ctcgtggtcg tgggtgtggc ggtgctcgtc gagcacgtcc cccgcatcgc catgctcgtg
     3481 ggcgtgcacg tgaaacacgc gaatgagcag aaacatggtg agcaggccgc ccagggtcca
     3541 cgccgccgca cgatcgaccg agccctgctc ggccacggaa tgtggcagca ggtgaagcac
     3601 ggccacgccg agcatgagcc cgccgacgaa gctcatcacg agctgcattt gccggtgcgt
     3661 gagtcggaac cagccgggca gcgctcctcc gagcagggaa gcgaccacga tcaagctgca
     3721 atagattgtc aagagcagca gaattgtcat aaggcgtcat cttaagccgg ggccgcacaa
     3781 gtcgcctaga tcacgcctgc atcgacattt ggcgcttgcc gtcgcccgcg ccggcatagc
     3841 ttttgcttcg gtcggtggga gttgcttggg cgagagcgag gagcctccgt gaaggtcacc
     3901 gtgttggcga acctggagaa cgggaagtcg gagcccgacg ccgtcgttcc gcagattacg
     3961 cgggcgctgt tggcccgcgg cgacaagacc aacgtcgtgg cgaccttcga cgacgtcgag
     4021 caattggttc gcgacctgaa agcggccaag acggacctgg tgttcaacgt attcgagtcg
     4081 ttcggccacg aggtgatcgg cgggcagatg ggcgtgacgg gcatcctcga tttgctgcaa
     4141 attccctaca ccggcggcgg acccggcgaa ctgtatttgc aggaagacaa ggccctcgcc
     4201 aaaaagctgt tggcctacga aaggatccaa tatcccgatt tcgcgatctt cgcaccgaat
     4261 gccgatttcg aaacgggagg caagctgcgg atgccgctgt tcgtgaagcc gctgcggatg
     4321 gatgcctcga tcgggatcga cgagaaatcg ctggtccgca atatgcagca gttgatggat
     4381 cgggttatga agattcacaa ggaactgggc gacgcggccc tggtcgaaga gttcatcgaa
     4441 ggccgcgagt tttacgtcgg cgtgctcggc aaccacgatc tgctggcctt tccgccaatc
     4501 gagatggatt tctccggcat gcccaagggc agcctgaagg tgatggacgc caaggccaag
     4561 ttcga
//
LOCUS       DASRJB010000487         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_135053, whole genome shotgun sequence.
ACCESSION   DASRJB010000487 DASRJB010000000
VERSION     DASRJB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135053"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFB80_12765"
     CDS             complement(<1..1124)
                     /locus_tag="VFB80_12765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN34689.1"
                     /translation="MKHVLLALALCLSAMSAAADDLPTTGEPRPELAAFDAWMQGFLQ
                     EHKIPGAALAIVKDGKLVYARGFGWADRDAKEQVEPDALFRIASVSKPITAVAILKLV
                     DQGKLKLDDKVLDHLKYDPHFEKDGKFDERWKQVTIRHCLAHTGGWDRGKSYDPMFQA
                     IRMAKSMEIELPILPEHIIRYQLGQPLDFDPGERYAYSNFGYSLLGRTIEKISGQPYE
                     KYVQEEVLKPLGITRARIGGSLESQRAEGEVRYYDARNGEGTAIVGLGAGVKKVPISY
                     GTWRQETFDSHGGWIASAPDLARFAAAFDFNDSQDHTRADLLKAGSVRLMFGPVVKMT
                     SATAGKTGGDRSYSLGWMIEPGENQTFTARHSGSLACTASS"
     gene            complement(1336..1989)
                     /locus_tag="VFB80_12770"
     CDS             complement(1336..1989)
                     /locus_tag="VFB80_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34690.1"
                     /translation="MTQQEIVSLVVFVSMCVVCCFTGAVVRRWLLNRCFPAFRFEPWL
                     FEPIRQSDLTDEQRQFFAKHTPVYLARGFEPLGDFLLRRDARPSVVRFFLSRDRTTVG
                     ELTWYLDTTTIGCMSVLLDGFFLESGTGEVQVLPPAEHGLQFFMLPQEDAPGLIDLHA
                     ASVAGVAAERRTSPAKIERDDVKALANYGRELSLRSLHKQGVIDELPAFLRKKQAAC"
     gene            complement(2104..>2586)
                     /locus_tag="VFB80_12775"
     CDS             complement(2104..>2586)
                     /locus_tag="VFB80_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN34691.1"
                     /translation="NFELLVTIDCDGQHEPQRIPLFFDACTPDVDIVSGSRYLEIFAG
                     DSAPPAQRRKINEQVTAELNRRLGLNLTDAFCGFKCYRADGLRKLHITEPGYAMPLEV
                     WVQAAAAGLKIIELPVPLIYLDEKRSFGGVLDDAQTRLEYYHLTLDRAIAAAPAGCCG
                     "
BASE COUNT          450 a          841 c          840 g          455 t
ORIGIN      
        1 gacgaggccg tgcaggccag cgagccgcta tgccgggccg tgaaagtctg gttctcgccc
       61 ggctcgatca tccagcccag gctgtaagag cggtcgccgc cggtcttgcc ggccgtcgcg
      121 gatgtcatct tgacgacggg accgaacatc agccgcacgg agccggcctt gagcagatcc
      181 gcgcgcgtat gatcctggct gtcgttgaag tcgaacgccg cggcaaagcg ggccaggtcc
      241 ggggccgagg cgatccagcc gccgtgcgag tcgaatgtct cctgccgcca ggtgccgtag
      301 ctgatcggca ctttcttcac gccggccccc aggcccacga tcgcggtgcc ttcgccattg
      361 cgggcgtcgt agtaccgcac ttcgccctcg gcccgctggc tttccagcga gccgccgatc
      421 cgcgcccgcg tgatgcccag cggtttcagg acttcctcct gcacgtactt ctcgtacggc
      481 tggccgctga tcttttcgat ggtccggccg agcaggctgt agccgaagtt ggaataggcg
      541 tagcgctcgc cgggatcgaa atcgagcggc tgcccaagct ggtagcggat gatgtgctcc
      601 ggcaggatcg gcagctcgat ctccatcgat ttggccatgc ggattgcctg gaacatcgga
      661 tcgtaggact tgccgcggtc ccagccgccg gtgtgtgcca ggcagtgccg gatcgtgact
      721 tgcttccagc gctcgtcgaa cttgccatcc ttctcgaagt gcgggtcgta tttcaggtgg
      781 tcgagcactt tgtcgtcgag cttcagcttc ccttgatcga cgagcttgag gattgcgaca
      841 gccgtgatcg gcttgctcac gctggcgatg cgaaacagcg cgtcgggctc tacctgctcc
      901 ttggcgtcgc ggtcggccca gccgaagccg cgggcataga ccagcttgcc gtccttgacg
      961 attgccagcg cggcccctgg aatcttgtgc tcctgcagaa agccctgcat ccacgcatcg
     1021 aacgcggcca actccggccg cggctcgccg gtggtgggga gatcatcagc agccgcggac
     1081 atcgcggaca ggcagagggc cagggccaac agcacgtgtt tcatgggata cctgcagtcg
     1141 tttcaagtcg ctcaatctaa cgcgtggtgt agcggcgcaa gaggagcagg cagtatgggc
     1201 ggatttgctc ccggctatca agttcctctt cgcgctgccg tgcctccttg cctcaatgcg
     1261 agatcaggcc cctgcgcggc ccaatcctgc gcggcccaat cctgcgcggc ccggtccagg
     1321 aacatgttgg atcggctaac aggccgcctg cttcttgcgc aggaatgcgg gcaactcatc
     1381 gatgacgccc tgcttgtgca gcgagcgcag cgacagctcc cggccgtagt tcgccagggc
     1441 cttcacgtcg tcgcgctcga tttttgccgg ggacgtcctg cgctctgctg ccacgccagc
     1501 aacgctggct gcgtgcaggt cgatcagccc cggcgcgtcc tcttgcggca acatgaagaa
     1561 ctggagcccg tgctcggccg gcggcagaac ctgcacttca ccggtgccgg attccaggaa
     1621 aaagccgtcg agcagcaccg acatgcagcc gatcgtcgtg gtatcgaggt accaggtcag
     1681 ctcgccgacc gtcgtccggt cgcggctgag gaagaagcgg accacggagg gccgggcgtc
     1741 gcgcctaagc aggaagtcgc ccaggggctc gaatccacgg gccagataaa ccggcgtatg
     1801 cttcgcgaag aactgccgct gctcatccgt caggtccgac tggcgaatcg gctcgaacag
     1861 ccagggctca aaccggaatg ccgggaagca gcggttgagc agccagcgcc gcaccactgc
     1921 gccggtgaaa cagcacacca cgcacatcga cacaaaaacc accagcgaga cgatttcctg
     1981 ctgagtcatc gcgcacccca aggcgaggcg gaagcgcaca agcctaggtc acgccgcggg
     2041 ccggattgtt ccggatttga cgaaggtgcg cttcaagcgg ctggaagcag cgtcggctac
     2101 ggactacccg cagcagccag cgggtgcggc ggcgatggcg cgatcgagcg tgaggtggta
     2161 atactcaagc cgcgtctgag cgtcgtcgag gacgccgccg aacgagcgct tctcgtccaa
     2221 gtatatgagc ggtaccggca gctcgatgat cttcagcccc gcggccgcgg cctggaccca
     2281 aacctccagc ggcatcgcgt agcccggctc ggtgatgtgc agcttccgca gcccgtccgc
     2341 gcggtagcac ttgaagccgc aaaaggcgtc ggtcaggttg agccccaggc ggcgattgag
     2401 ctccgccgtg acttgctcgt tgatcttgcg gcgctgggcc ggcggggcgc tgtcgcccgc
     2461 aaaaatctcc aggtagcggc tgccggacac aatatcgacg tcgggcgtgc aggcgtcgaa
     2521 gaacagcgga atccgctgcg gctcgtgctg tccgtcgcag tcgatcgtaa cgagcagctc
     2581 gaaatt
//
LOCUS       DASRJB010000488         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1826384, whole genome shotgun sequence.
ACCESSION   DASRJB010000488 DASRJB010000000
VERSION     DASRJB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826384"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VFB80_12780"
     CDS             <1..1283
                     /locus_tag="VFB80_12780"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZN34692.1"
                     /translation="LRVLYLDGEKRFEQKFLRASLNASPDIELDDRIIDRRNQASQYD
                     ITGDLRTGRYDAFVIGDLDAELLTPESVKLLAEQVNAGKGLVMIGGETSFGWGHYRGT
                     ALEEVLPIEMDPIEGTQLSPQAQDRFFRDGPVPLAPADSHSITRLAPAAENDSVWKKL
                     TPLNWVHRFAGVKQAPGVRVLLETPQQEPVLVSGEFGRGRVLALGTESTWLWQMHGFE
                     KEHKRFWRQIILWLVRRDDLARDDVWVKLEQRRFNPGSRVQVTAGARTSAGDVIADAQ
                     IDAAVTLPSGGKQTLKLAKDREGNFAGSLEVTEPGDYAIEVAATRQGQTLGSARAEFL
                     VFDRDVELSNIAADPLFLDSLARETRHEGGKLVLPEQFPVLLGEYADRPKEYEERQIK
                     WKLASTGADAWAFLLTLAVTLSLEWYLRKRWGLV"
     gene            complement(1300..>2269)
                     /locus_tag="VFB80_12785"
     CDS             complement(1300..>2269)
                     /locus_tag="VFB80_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN34693.1"
                     /translation="WRGRAGTGGLMTGACLLVALAVVATPADGLPAAPVTALAFAPDG
                     TSVLAATPHEVAELSWPELKLRRKIVADLNHVHDLAFAPRGKALAIAGGKPGESGRVA
                     LVSWPEGMPREPIDAGSDVIYRAAWFPDGSKLALACADKCVYVAAPGGHAELTFQEHS
                     APVLSAIVIPLANRLLLLSGGRDQTIRVWNADQSDKSLRSFDNHTAAVYDLALRPAAD
                     DLPPIVASAGADRTVRFWQPTIGRMLRFSRLASPPLAVCWTADGQQVCAACQDGRVRI
                     VELQTARVVAEHDAIDGWAHSIILAPDGKSILVGGEGGQLKVITQH"
BASE COUNT          407 a          749 c          759 g          354 t
ORIGIN      
        1 gcctgcgcgt gctctatctc gacggagaaa aacgcttcga gcaaaaattc ctccgcgcca
       61 gcctcaacgc ttcgcccgac atcgagctcg acgaccgtat tatcgaccgc cgcaatcagg
      121 ccagtcagta cgacatcacg ggcgaccttc gcaccggccg ctacgacgcc ttcgtgattg
      181 gcgacctgga cgccgagctg ctcacgcccg aaagcgtgaa gctcctggct gagcaggtga
      241 atgccggcaa ggggctggtg atgatcggcg gcgaaaccag ctttggctgg gggcactatc
      301 gcggcacggc gctcgaagag gtgttgccca tcgaaatgga ccccatcgaa gggacgcagc
      361 tcagccccca ggctcaggat cgcttcttcc gcgatggtcc ggtgccgctg gcgccggccg
      421 attcgcattc gatcacacgg ctggcgccgg ccgccgagaa cgactcggta tggaagaagc
      481 tgacgccgct gaactgggtg caccggtttg ccggcgtcaa gcaggccccg ggcgtgcggg
      541 tgctcctgga aacgccgcag caggagccgg tgctcgtgtc gggcgagttc ggccgcggcc
      601 gcgtgctggc tctggggacc gaatcgacgt ggctctggca aatgcacggg tttgaaaagg
      661 agcacaagcg gttctggcgg cagatcatcc tgtggctagt gcggcgagac gacctggccc
      721 gcgacgacgt gtgggtcaag ctcgagcagc ggcggttcaa ccccggcagc cgcgtgcagg
      781 ttacggccgg ggcgcgaacg agcgccgggg acgtgatcgc ggacgcacag atcgacgccg
      841 ccgtcacgct ccccagcggc ggaaagcaaa cgctgaagct ggccaaggac cgggagggga
      901 acttcgccgg ctcgctggaa gtgaccgagc cgggcgacta tgccatcgaa gtggccgcca
      961 cgcgccaggg gcagacgcta ggaagcgccc gtgcggagtt cctcgtcttt gaccgggacg
     1021 tcgagctgag caacatcgcg gccgatccgc tgttcctcga ctcgttggcc cgcgagacgc
     1081 ggcacgaagg gggcaagctc gtgctccctg agcaatttcc cgtgctgctc ggcgagtatg
     1141 ccgaccgccc caaggaatac gaagagcggc agatcaagtg gaagctggca tccaccggcg
     1201 cggacgcctg ggcgtttttg ctgacgctgg cagtgactct gtcgctcgaa tggtatctgc
     1261 gaaagcggtg ggggctggtg tgaatcagcg cggcgtgact cagtgctgcg tgatgacctt
     1321 gagctggccc ccctcgccgc cgaccaatat cgacttgccg tcgggcgcga ggataatgct
     1381 atgggcccag ccgtcgatcg cgtcgtgctc ggcgacgacg cgggcagtct gcaattcgac
     1441 aatccgcaca cggccgtcct ggcaggccgc gcagacttgc tggccgtctg cggtccagca
     1501 aactgccagc ggcgggctcg ccagccggct gaagcgcagc atgcgcccaa tcgtcggctg
     1561 ccagaagcgg accgtgcgat cggcccccgc cgaggccacg atcggcggca aatcgtccgc
     1621 cgctggccgc agcgccaggt cgtaaaccgc cgctgtgtgg ttgtcgaagc tgcgcagtga
     1681 tttgtcggat tgatccgcat tccacacccg gatcgtctga tcgcgcccgc ccgaaagcag
     1741 tagcagtcga ttcgccaacg ggatgacaat tgccgaaagc acgggcgcgg aatgctcctg
     1801 gaatgtgagt tccgcatggc caccgggggc cgcgacgtac acacacttat ccgcacacgc
     1861 cagcgccagc ttgctgccgt ctggaaacca ggccgcccga tagatcacgt cgctccccgc
     1921 atcgatcggc tcacgcggca ttccttccgg ccagctcacc agcgccacgc gtcccgactc
     1981 gcccggtttg ccgccggcaa tggccagcgc cttgccgcgc ggggcaaagg cgagatcgtg
     2041 aacgtggttg agatcggcga cgatcttgcg ccgcagtttg agttcgggcc aggacaattc
     2101 ggctacctcg tgcggcgtgg ccgccaggac cgacgtccca tcaggtgcaa aggccagcgc
     2161 ggttaccggc gcagcgggca ggccgtctgc cggtgtcgcg acaacagcca gcgccacgag
     2221 caagcacgcg cccgtcatca ggccaccagt tcccgcacga cctcgccat
//
LOCUS       DASRJB010000489         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3800525, whole genome shotgun sequence.
ACCESSION   DASRJB010000489 DASRJB010000000
VERSION     DASRJB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800525"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFB80_12790"
     CDS             complement(<1..932)
                     /locus_tag="VFB80_12790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN34694.1"
                     /translation="MSQPLLSVIIPTYNRAELVRECLASLRAAGVGSQASGIGGRETG
                     PTTDHRPPTPDSRLEVIVADDGSTDDTGEVVEREWPGAKYLWQPNSGTPATARNLGFA
                     ESRGKYVAFVDCDDRWLPGVAGEVLRLLEEHPQIDVLFTEARMGNETEGYRSWIEMGG
                     EQAFFELPCERPAAGLRSDAGGGLRVLARRPFFRRMAVRNAVFISAVVMRREAFERSG
                     GFDPALRGAADWELWLRMASQMTFAYFEQPLAVYTRHLDGAANMSSDHDGMGAEFIRA
                     LEGVLRKCPHLPAEDRRLVERQLKHHLFGHAYAA"
     gene            complement(929..2095)
                     /locus_tag="VFB80_12795"
     CDS             complement(929..2095)
                     /locus_tag="VFB80_12795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN34695.1"
                     /translation="MATDLISRGLSDTSAPANRPLRVGFSELHGMAHELCQFPPEGVT
                     CSEVVGRPVRLSPIRSEIKGYMRRCESAEHDVIEAVISPIVTRNNWVYSVECMQAAMS
                     FSLLGCPLPRSVRAAWFRRLLLQPNLKKIVFWSQAGRRTLESYAGIRDERIVRKATVV
                     YPAIRRVSDELIRFSDGDVQILFSGDFFRKGGVHVVEAFERAQEQYRGIRLRLCCDEK
                     IDFSTRDAALRDEFLGRIRRNPGIVFGRVPRDEMIRQVLPATDIYALPTYVETFGFAL
                     LEAMAFGIPVISTNHFAIPEIVEHGECGFLIDTRRFDCERLFRGYRVDAIPRGFREYM
                     TGEVYLRLCELIESVDLRRRLGTAGVQIARTKFSLDARNERMLGIYREAVGEAA"
     gene            complement(2111..2923)
                     /locus_tag="VFB80_12800"
     CDS             complement(2111..2923)
                     /locus_tag="VFB80_12800"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN34696.1"
                     /translation="MSAANQTALQLVIGLYRFLFAREFFYPLNKLLFALSARGLGLLN
                     FEDERASGESHLIRRVLPRLIRREEPVVFDVGANVGSYAAAVLDRFPRARVHAFEPHP
                     ATFITLERRDSRIVAHRLALGETRGTVTLYDRNDCAGSQQASIYGEVISELYHQDPLP
                     VEVPIDTIDNVVAQQQVDFVDFLKIDTEGHELAVLKGASQTLACGKIGCIHFEFNEMN
                     VVSRVFFRDFRMLLAGYKFFRLLPSGLLRLGENPLQTELFAYQNILAVKADS"
     gene            complement(2920..>3445)
                     /locus_tag="VFB80_12805"
     CDS             complement(2920..>3445)
                     /locus_tag="VFB80_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN34697.1"
                     /translation="VGIDPSANVLENPLLDEREQTGIEGYRAREPFDLVTLRMVVEHV
                     PDPQACVAAIAQNARPGGVVVVYTPHKYSLLSIASALVPQRAHHALKSRLWGTEERDT
                     FPAFYRMNTPGMLRRLFEAGGFRTLAMWLLDDATVFHSRPRLGPAELAFWSLTRRLRL
                     PYPERNILAVFERA"
BASE COUNT          639 a         1257 c          994 g          555 t
ORIGIN      
        1 taagccgcat acgcgtggcc gaacaggtga tgcttcagct gccgctcgac cagccgccgg
       61 tcctcggccg gcaagtgcgg gcacttccgc agcacacctt ccagcgcccg gatgaactcc
      121 gctcccatcc cgtcgtggtc actggacatg ttcgccgcgc cgtccaaatg ccgcgtatag
      181 accgccaacg gctgctcgaa ataggcgaac gtcatctggc tggccatccg cagccacagc
      241 tcccagtcgg ccgccccgcg cagggccgga tcgaaaccgc ccgaccgctc gaacgcctcc
      301 cgccgcatca ccaccgcgct gatgaacacc gcgttccgca ccgccatccg ccggaagaac
      361 ggccgccgtg ccagtacgcg cagcccgccg ccggcatcac tgcgcaggcc cgccgcgggc
      421 cgctcgcagg gcagctcgaa aaacgcctgc tcgcccccca tctcgatcca cgaccggtaa
      481 ccctcggtct cgttccccat ccgggcctcg gtgaacaaca cgtcgatctg cggatgctcc
      541 tccagcagcc gcagcacctc ccccgccaca cccggcaacc agcggtcgtc gcaatcgacg
      601 aacgccacgt acttcccgcg gctctccgca aaccccagat tccgcgccgt cgccggcgtc
      661 ccggaattcg gctgccacaa atacttcgcc cccggccact cgcgttccac cacctccccc
      721 gtgtcatcgg tcgagccgtc atcggccacg atgacctcga gtcgggagtc gggggtcggt
      781 ggtcggtggt cggtagtcgg tcctgtctcc cgccccccga ttcccgacgc ctgactcccg
      841 actcccgccg cccgcagcga cgccaaacac tcccgcacca actccgcgcg gttgtacgtg
      901 ggaataatga ccgacagcag cggctggctc atgccgcctc cccgaccgct tcgcggtaga
      961 tgccgagcat ccgctcgttg cgggcgtcga gcgagaactt cgtgcgggcg atctgtaccc
     1021 cggccgttcc caaccgccgc cgcaaatcga ccgactcgat cagctcgcac agccgcaggt
     1081 aaacctcccc ggtcatgtat tctcgaaaac cgcgcggaat cgcatcgacc cggtagccgc
     1141 gaaacagccg ctcgcagtcg aaccttcgcg tatcgatcaa aaatccgcac tccccatgct
     1201 cgacgatttc ggggatcgcg aaatggttcg tcgaaatcac cggaatgccg aaggccatcg
     1261 cctccagcag ggcgaaccca aacgtctcga cataagtcgg caaggcatag atatccgttg
     1321 cgggcaggac ctgccggatc atctcgtcgc gcggcacgcg cccgaagacg atgcccggat
     1381 tgcggcggat ccgccccagg aattcgtccc gcagggcggc atcgcgggtg ctgaagtcga
     1441 tcttctcgtc gcagcacagc cgcaggcgga tgccgcggta ttgctcctgg gcccgctcga
     1501 aggcctcgac gacgtgcacg cctcccttgc ggaagaagtc gccgctgaac aggatttgca
     1561 cgtccccgtc gctgaaccgg atcagctcgt cgctcacgcg gcggatcgcc ggatagacga
     1621 ccgtcgcctt ccgcacgatc cgctcgtcgc gaatgccagc gtacgattcg agcgtccgcc
     1681 gccccgcctg gctccagaac acgatcttct tcaggttcgg ctgcaagagc agccgccgga
     1741 accaggccgc ccgcacactc cgcggcagcg ggcaccccag caggctgaac gacatcgccg
     1801 cctgcatgca ctcgaccgaa tagacccagt tgttccgcgt cacgatcggg ctgatcaccg
     1861 cctcgatcac gtcgtgctcg gccgactcgc agcgccgcat atatcctttg atctcgctgc
     1921 gaatcgggct cagccgcacc ggccggccca ccacctccga gcaagtcacc ccctccggcg
     1981 gaaactggca cagctcatgc gccatcccat gcagttcgct gaagccgacg cgcagcggcc
     2041 ggttggcggg agccgacgta tcgcttaggc cgcgggaaat caggtcagtt gccacagaag
     2101 ccatgccgcc ctagctgtcc gcctttaccg ccaaaatgtt ctggtacgca aacagctccg
     2161 tctgcagtgg gttctcgccc agtcgcagca gcccgctcgg cagcagccgg aaaaacttgt
     2221 atcccgcgag cagcatccgg aagtcccgaa agaaaacgcg gctgaccacg ttcatctcgt
     2281 tgaactcgaa atggatgcag ccgatcttgc cgcaggccag cgtttgcgac gctcccttga
     2341 ggaccgccag ctcgtgccct tcggtgtcga tcttgaggaa gtccacgaaa tcgacctgct
     2401 gctgcgcgac cacgttgtcg atcgtgtcaa tcggcacttc caccgggagc ggatcctggt
     2461 ggtacagctc agaaatgact tcgccataga tcgaagcttg ctgtgatccg gcgcagtcat
     2521 tgcggtcgta tagcgttacg gtgccgcgcg tttctccgag ggccagccga tgcgcgacga
     2581 ttcgtgagtc gcgccgctcc aaggtgatga acgtcgccgg atggggttcg aaggcgtgga
     2641 cgcgcgcccg cggaaagcga tcgaggaccg ccgccgcgta acttccaacg ttggccccga
     2701 catcgaaaac gaccggctct tccctcctga tcagccgcgg cagcacgcgg cgaatcaagt
     2761 gcgattcgcc gctcgctcgc tcgtcctcaa agttcagcag gcccagtcct cgcgcgctca
     2821 gggcgaatag gagcttgttg agtgggtaga agaactcccg cgcaaacagg aagcggtaca
     2881 aaccgataac cagttgcaac gccgtttgat tcgcagcgct catgcccgct cgaagaccgc
     2941 caggatgttc cgctcgggat agggcagccg cagccgccga gtgagcgacc agaaggccag
     3001 ttccgccggg ccgagccgcg ggcgggaatg aaacacggtc gcgtcgtcca gcagccacat
     3061 tgccagcgtg cggaagccgc cggcctcgaa cagccggcgg agcatccctg gcgtattcat
     3121 ccgatagaaa gccggaaacg tatcccgctc ctcggttccc cacagccggc tcttcagcgc
     3181 atgatgcgcc cgctgcggca cgagcgccga ggcgatcgac agcagcgaat acttatgcgg
     3241 cgtgtagacc actacgacgc cgcccggccg cgcgttttgc gcgatcgccg ccacgcaggc
     3301 ctgcggatcc ggaacgtgct cgaccaccat tcgcaacgtg accaggtcaa agggctcgcg
     3361 cgcgcgatac ccttcgatcc cggtctgctc acgctcatcg agcagcggat tctccaggac
     3421 gttcgcgctt ggatcgattc ccacc
//
LOCUS       DASRJB010000490         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_985841, whole genome shotgun sequence.
ACCESSION   DASRJB010000490 DASRJB010000000
VERSION     DASRJB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985841"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFB80_12810"
     CDS             <1..1177
                     /locus_tag="VFB80_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34698.1"
                     /translation="AGVRSVTGEIPTAIPGVTFGSTFTRLAGLADKLAIVRSFRTGDA
                     NHDIKPVVGKATGGANMGSLFARVAGTNDPQTGMPLNAALFPRAVEPMAGAAVEQFGN
                     FLATGGLGAAYSPFVPGGSGAAQKDMELKLDPTRLGDRKSLLSNLDRIRRDLDAAGAL
                     PGVDRFQEQALQTILGGVAKAFDVSQEDAKTIARYDTAPLVRPEQISRKWNNYPRYVD
                     NAKTLGRLLLLARRLCEAGCGFVTVTTNFVWDMHADQNNAGVEEGMQYMGRPFDHAVA
                     SFIEDCEARGLSDKILLVATGEMGRTPKINKNGGRDHWGGLSPLLLYGGGLKGGQVIG
                     SSTRDAGEPLSEPYGNDNLLATVMHTLLDIGQVRLLTGLPQDLLRTLTGAEPIKGLA"
     gene            1295..1891
                     /locus_tag="VFB80_12815"
     CDS             1295..1891
                     /locus_tag="VFB80_12815"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN34699.1"
                     /translation="MIQIAPRATIADLYHVEGKAELIGGKIVHLMASGFQPSEVAFLI
                     AISLRAFALRIGVGVALPDGIGYAIRPPLSNGRESFSPDASYYAGPLPSDPMRFIEGA
                     PTFAVEVRSEEDYGPAAERALADKRADYFEAGTKAVWDVDPKAKTIASYRNPSLQRDK
                     TGNGTSNPLVFHIGDNANAEPAVPGWTMAVIDCFNPAA"
     gene            complement(1954..2976)
                     /locus_tag="VFB80_12820"
     CDS             complement(1954..2976)
                     /locus_tag="VFB80_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34700.1"
                     /translation="MSRSRAEQFEAPGKDSFLDVVANVVAILIILVIVVGSQASKAAV
                     QKETARLAAQASGGGAGQAEAGSVASEAKALESGIQDLQSTIDRQKLEVAFRRAERDR
                     VQLLVAAAERRLAEHREQLSAAEKERYDLDAELIASRGELTSLAQAEARPGKPPRIVL
                     QHLPTPMAKTVFGDEVHFRLQGHRLAYLPWDEMMAQLKADAPNQVHKLRSQPRVEVSL
                     PPISGFGARYALKVATQEMQTRLGVATQSQVVLDRIFFVDAEPNLGQPAAEAFAPTSE
                     FRSRLAGHKPQATTITIWVYSDSFDDYRALKAELFKLGYLCAARPMPDGHPIGAAPDG
                     TRSSAE"
     gene            complement(2973..3299)
                     /locus_tag="VFB80_12825"
     CDS             complement(2973..3299)
                     /locus_tag="VFB80_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34701.1"
                     /translation="MPGSRGRVTAVTRMIHLAVLSDRIVIVPEQGDERLPKHLPVSPE
                     LTPAEVDRFVAAVQREIQGWGLAVEDGYWKPLLLVEVAPDAESQAQQLETALQGSGFD
                     IQRKLR"
BASE COUNT          602 a         1349 c         1254 g          622 t
ORIGIN      
        1 ggccggggtc cgcagcgtca cgggcgaaat tcccacggca atcccgggcg tcaccttcgg
       61 cagcacgttc acgcggctgg cgggcctggc cgacaagctg gcgatcgtcc gcagcttccg
      121 caccggcgac gccaaccacg acatcaagcc cgtcgtgggc aaggcgaccg gcggtgcgaa
      181 catgggttcg ctcttcgccc gcgtcgccgg cacgaacgat ccccagaccg gcatgccgct
      241 gaacgccgcg ctctttccgc gggccgtcga gccgatggcc ggcgcggccg tggaacagtt
      301 cggcaacttc ctggcgacgg gcgggctcgg cgcggcttac tcgccgtttg tgcccggcgg
      361 cagcggcgcg gcccagaaag acatggagct caagctcgat ccgacccggt tgggcgaccg
      421 caagagcctg ctctcgaacc tcgaccgcat ccgccgcgac ctcgacgccg ccggcgcatt
      481 gccgggcgtg gaccgctttc aggagcaggc gctccagacg atcctcggcg gcgtcgccaa
      541 ggccttcgac gtttcgcagg aggacgcgaa gacgatcgcc cgctacgaca cggccccgct
      601 cgtccgcccc gagcagatca gccgcaagtg gaacaactac ccgcggtacg tcgataacgc
      661 caagacgctc ggccggctgc tgctcctggc ccgccggttg tgcgaagcgg gctgcggctt
      721 cgtgacggtc acgacgaact tcgtgtggga catgcacgcc gatcagaaca acgccggcgt
      781 cgaggaaggc atgcagtaca tggggcggcc gttcgaccac gcggtagcca gcttcatcga
      841 agactgcgaa gcccgcggct tgtcggacaa gattctgctg gtggccacgg gcgagatggg
      901 ccgcactccc aagatcaaca agaacggcgg ccgcgaccac tggggcggcc tctcgccgct
      961 cttgctctat ggcggcggcc tgaagggcgg ccaggtgatc ggcagttcga cgcgcgacgc
     1021 gggcgaaccg ctctccgagc cctacggcaa cgacaacctc ctggccaccg tcatgcacac
     1081 gctcctcgac atcggccagg tccgcctgct gaccggcctg ccgcaggacc tcctccgcac
     1141 gctgaccggc gcggagccga tcaagggact ggcatagcat tgtcagccgg atatctgctg
     1201 tccgctggag gaaccgttca aacttgctcg ctgcatggca gcgggatagg cttgaggata
     1261 tcgaggtaat ctccgccgcc acggtagcat cgagatgatt cagattgccc ctcgggccac
     1321 gattgccgat ctctatcacg tcgaggggaa ggcggagctg atcggcggca agatcgtaca
     1381 ccttatggcc agcggctttc agccctcgga agtggcgttc ttgattgcaa ttagcctgcg
     1441 ggcgtttgcc ctgcggatcg gcgtaggagt ggcgctcccc gatggcatcg gctacgccat
     1501 tcggcccccg ctgtccaacg gacgcgaatc gttttctccc gacgcttctt actacgctgg
     1561 tccgctgcca agcgacccga tgcgcttcat cgagggcgcg ccgacctttg ccgtggaagt
     1621 gcgcagcgaa gaggactacg gccccgccgc cgaacgcgcg ctggccgaca agcgcgccga
     1681 ctacttcgaa gccggcacca aggccgtctg ggacgtggat cccaaagcca agacgatcgc
     1741 cagctatcgc aacccgtctc tgcagcgcga caagacaggg aacggaacgt ccaatcctct
     1801 cgtctttcac atcggcgaca atgccaacgc cgaacctgcc gtacccggct ggaccatggc
     1861 cgtcattgat tgcttcaacc ccgctgccta aagccggcgg tgcgatcaac gactttgtgg
     1921 ctttggcacg atttgcgcaa atgagaaact ggctcactcc gccgacgacc gcgtgccgtc
     1981 gggggcggcg ccgatggggt ggccatcggg catggggcgg gcggcgcaaa ggtagccgag
     2041 cttgaacagc tcggccttga gtgcgcggta atcgtcaaag ctgtcggaat agacccagat
     2101 cgtgatcgtc gtggcttggg gcttgtggcc cgccagccgg ctgcggaatt ccgacgtggg
     2161 cgcgaacgcc tcggctgcgg gctggcccaa attcggctcg gcatcgacga agaaaatccg
     2221 gtccagcacg acctgcgact gcgtggccac gcccagccgc gtctgcattt cctgggtggc
     2281 gaccttcagc gcgtaccgcg caccaaatcc ggagattggc ggcagcgaca cctcgacccg
     2341 cggctgcgag cggagcttgt gaacctggtt gggagcgtcg gcctttagct gagccatcat
     2401 ttcgtcccag ggcagatacg ccaggcgatg gccctgcagg cggaagtgga cttcgtcgcc
     2461 gaacaccgtc ttcgccatcg gcgtgggcag gtgctgcagg acgatccgcg gcggcttgcc
     2521 gggcctggcc tcggcctggg ccaggctcgt caattcgccg cgcgacgcga tcagctcggc
     2581 gtccaggtcg taccgctcct tctcggcggc cgacagctgc tcacggtgtt cggccagccg
     2641 ccgctcggct gccgccacca gcagctgcac gcgatcgcgc tcggcccggc gaaaggcgac
     2701 ctccagcttt tgtcggtcga tcgtggactg caagtcttga attccggact cgagggcctt
     2761 ggcttcggac gccacgctgc cggcttccgc ctgccccgcg ccgccgccag aggcctgagc
     2821 cgccaggcgg gccgtttctt tctgcacggc cgccttgctg gcctgcgagc cgacgacgat
     2881 gaccagaatg atgaggatgg ctacgacgtt ggccacgaca tccaggaagg agtctttgcc
     2941 cggcgcttcg aattgttcgg cccgcgaccg gctcaccgaa gtttcctctg aatgtcgaag
     3001 ccgctgcctt gcagggcggt ttccagctgc tgcgcttggg actcggcgtc gggcgcgacc
     3061 tcgaccaaca gcagcggctt ccaatagccg tcctcgaccg ccagacccca gccttgaatc
     3121 tcgcgctgca cggcggcgac aaagcgatcg acctcggcgg gcgtcagctc gggcgagacc
     3181 ggcaggtgtt tgggcagccg ctcgtcgcct tgttccggca cgatcacgat ccggtcgctg
     3241 agcaccgcca ggtggatcat ccgcgtgacc gccgtgacgc ggccgcgcga gccgggcaag
     3301 ccccaattgt tgccgcgcgc ggcattgcct tgtgcacttc cgccgccgga ggcttgactg
     3361 ccgggcctgc cgctgccagc ccctccgggt ggtccgggcc gcggccggcc gtaagcgagt
     3421 ttgccggcag gctgtccgcc tcctccgcct ggcacagctc cggcgcttgt tggtccaccg
     3481 ccagcgcctg gcgcctgacc ctgaggaccc gcgccagcgg tcgcgtttct gctggcagca
     3541 tttcctccgg cgccgccagg gaacggtctg ggcggctcgc ttccgcccgg cgcgccgact
     3601 gctccgccgc cactgcctgc cggagcgcca ccagacgtgc ctgccgggta gctctctagt
     3661 cccattgcat ctccgtgccc gccgccgcgg ccgccggcca gtccggtgcc gctgcctccg
     3721 ccgccggtgc ccaccccgcg acccggcgcg gaggaaccgg ccgcgccaag catcgggccg
     3781 ccctcgacgg gtgatgggcc gccgaacgat tcgccgtgga accgccg
//
LOCUS       DASRJB010000491         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3801231, whole genome shotgun sequence.
ACCESSION   DASRJB010000491 DASRJB010000000
VERSION     DASRJB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801231"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFB80_12830"
     CDS             <1..392
                     /locus_tag="VFB80_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZN34702.1"
                     /translation="EQDTLGRMKLRGVILSRIETELDGRLAHTHVLKDDFTRTGSLPS
                     DTEDVVNSALAIRGTQFAVIFVEQAGGGFKISFRSRCAVDCSKLAEQWGGGGHKAAAG
                     AFVAGPLAEVQPKVLDAVRKAMGNVVE"
     gene            798..1916
                     /locus_tag="VFB80_12835"
     CDS             798..1916
                     /locus_tag="VFB80_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN34703.1"
                     /translation="MKITAVVCQILRIPQVQAKTASSQDSVLVRVRTDEGLEGVGEAD
                     SSPEVVKAIVDAPFSHNIACGLRQIVLGENPLEHERLWQKMYRRTMYFGRTGVGITAM
                     AAVDMALWDLKGKAFGQPIHRLLGGKQHEKLRAYASILFGKDGRETAEIARRWVGEGY
                     TAVKFGWEPMGQSERLDRELAAGAREGLGQHTLLIDAGCVWDARTALQRAQAFAEYRA
                     EWLEEPLREDDIEGYVWLRDRSPIPIAAGEGECGRESFRPLIDRRALDIYQVDLSRCG
                     FTDAAYIKSRVEEIGARLCNHCYTSPITAAASLHWLSTCRDAFLFEDCVDDSPLRHEL
                     THERVQAEGGWIRVPDGPGLGVTLNEEFVRAHLVAESR"
BASE COUNT          391 a          638 c          709 g          356 t
ORIGIN      
        1 acgagcagga cacgctcggc cggatgaagc tccgcggcgt gatcctgtcg cgaatcgaga
       61 cagaattgga cgggcgcctg gcgcatactc atgtgctgaa ggacgatttc accaggaccg
      121 gttcgctgcc cagcgacacg gaggacgtag tcaactcggc cctcgccatc cgcgggacgc
      181 agttcgctgt gatcttcgtc gagcaagccg gcggcggctt caaaatcagc tttcgcagcc
      241 gctgcgccgt cgattgcagc aagctggccg agcaatgggg cggcggcggg cacaaggcgg
      301 cggccggcgc attcgtcgcc ggtccgctgg ccgaagtgca gccgaaggtt ttggatgcgg
      361 tgcgcaaggc gatgggtaac gtcgtggaat aattcgccgc gagttgcagc tccgtggcga
      421 attaatcagg cagtggtgag cgtcgcggaa atccgcaagt gccgccccgc aatcgagttg
      481 cggcattcga cggcgtcgct ggccggccga ataattcgcc aaacgcctca aacacgcggg
      541 gaagtggcga attattcggt cattttcgcc gcaacatact gccggtgcgt cagttgcggc
      601 gaaaaacgcg tggcgaataa ttcgctacgc gggatcaaat tgccaaggcg ccaaaatgac
      661 agtctcgctg ccaaagggaa ctatgtagca aataccatac caatgttcac acttcgccaa
      721 gaatctcatc ggccgcccga aatcggcctg ctatgctggc ggctcattgc aggcggtggt
      781 tgctcttaag gcggaccatg aagatcacag cagtcgtctg tcagatcctg cggattccgc
      841 aggtccaggc caaaacggcc agcagccagg actcggttct cgtccgcgtc cgcaccgacg
      901 agggcctgga gggcgtcggc gaggccgact cgtcgccgga ggtcgtcaag gcgatcgtcg
      961 atgctccctt cagccacaat atcgcctgcg gactgcggca gatcgtgctg ggcgagaacc
     1021 cgctcgagca cgagcggctg tggcagaaga tgtatcgccg cacgatgtat tttggccgca
     1081 cgggcgtcgg aatcacggcg atggcggccg tcgatatggc cctctgggac ctgaagggca
     1141 aggccttcgg ccagccgatt caccgcctgc tcggcggtaa gcagcacgag aagctgcggg
     1201 cttatgcgtc gatcctcttc ggcaaggacg gccgcgaaac cgccgagatc gcccgccgct
     1261 gggtgggtga aggttacacc gccgtcaagt tcggctggga gccgatgggc cagagcgagc
     1321 ggctcgaccg cgagctggcc gccggtgcac gcgaagggct gggccagcac acgctcttga
     1381 tcgacgccgg ctgcgtgtgg gatgcccgca cggccttgca gcgggctcag gcctttgccg
     1441 aatatcgggc ggaatggctg gaagagccgc tccgcgagga cgacatcgag ggctacgtct
     1501 ggctgcggga ccgctcgccg attccgatcg cggccggcga gggagagtgc ggccgcgagt
     1561 cgttccgccc gctcatcgac cggcgggcgc tcgacattta ccaggtcgat ctctcgcggt
     1621 gcgggttcac cgacgcggcg tacatcaaga gccgcgtcga agagatcggc gcgcggctct
     1681 gcaatcactg ctacaccagc ccgatcacgg cggcggcgag cctgcactgg ctcagcacct
     1741 gccgcgacgc gtttctgttc gaggattgcg tcgatgattc gccgctgcgg cacgagctga
     1801 cgcacgagcg ggtgcaggcc gaaggcggct ggattcgcgt gcccgacggc ccggggctgg
     1861 gcgtaacgct caacgaggaa ttcgtgcggg cacacctcgt cgcggagtcc cgctaggagt
     1921 tcagaattca gaatgcagaa ttcagcagcc cgaagttact ggccggtcgt aatcctgctg
     1981 atgctcgtcg tcggcctggg acacttcaac cgcgtcggca tgtcggtggc ggggaccgag
     2041 cggatcatcc cggagtacaa gatcgcgccg gaccagatgg gcctggtcta ctct
//
LOCUS       DASRJB010000492         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3518885, whole genome shotgun sequence.
ACCESSION   DASRJB010000492 DASRJB010000000
VERSION     DASRJB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518885"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFB80_12840"
     CDS             <1..1098
                     /locus_tag="VFB80_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34704.1"
                     /translation="SQSDRVTEIAGGRMIGLGLGVRLGRRVTTLSAYVQDADSGSLAA
                     VERSFADPAPDSGEEPKSYADLGGIVLSRGVSLAALSRSQLLLKSGKRTPSGRLVLPR
                     TAASLTINPQTFSWEQLKPPAAVESFAQLAARLASLPPSYLRPRRLTENVYACPVQAA
                     EELRFNESQQRLEAVLRDAAGGRALLVHPFHSRGKTGFGTLLGALTSRGPSVRFVSGH
                     VTGSREGLLIRPILVVLEDGDRRIGIQPLVSESAPGRSAPAPTPGEPEQDPRSPVQAD
                     PLESFLTQMRLELSELLLSGARRAGQPQARAWTDLATHGRELGFVRLVEPLARLAELL
                     AGRADALRWDPAPAARALMDLCVYARIASDA"
     gene            1264..1527
                     /locus_tag="VFB80_12845"
     CDS             1264..1527
                     /locus_tag="VFB80_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34705.1"
                     /translation="MAKRRNKAAKIRQMLAKHPDASAKEIVAALAAQRTRVTPAQVYN
                     VKSMQGKPKTGRYDTLLHAKKLADALGGIDKARQALDLLARLM"
     gene            2153..>2449
                     /locus_tag="VFB80_12850"
     CDS             2153..>2449
                     /locus_tag="VFB80_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34706.1"
                     /translation="MRSIFSCCWTAGLLVLALAAPSWGQDPFVGRIILVNDGQVAKDI
                     GLADADVAKLKEFIDHRLVEAQTLMNATKTAPAAEREAKLAEFLAESEKQGLELL"
BASE COUNT          441 a          796 c          783 g          429 t
ORIGIN      
        1 tcgcagtcgg accgcgtcac tgagatcgcc ggcgggcgga tgatcggcct ggggctgggc
       61 gtgcggctgg gacggcgcgt gaccacgctc agcgcgtacg tgcaggacgc cgattcgggc
      121 agcctcgccg ccgtggagcg ctcgttcgca gatcccgcgc cggattcggg cgaagagccc
      181 aagagctacg ccgatctggg cgggatcgtg ctctcgcgcg gcgtctcact cgcggccctg
      241 agccggtcgc agttattgct caagtcgggc aagcgcacgc cgagcggccg cctggtcctg
      301 ccgcgcaccg ccgcgtcgct gaccatcaac ccgcagactt tctcctggga gcagctcaag
      361 ccgccggccg ccgtcgagag cttcgcccag ctcgcggccc gcctggcgtc gctgccgcca
      421 tcgtacctgc ggccgcggcg gctcaccgaa aatgtctatg cctgccctgt gcaggccgcc
      481 gaggagctgc ggttcaacga atcgcagcag cgcctggagg cggtactgcg cgacgcagcg
      541 ggcgggcggg cgctgctcgt gcatccgttc cactcgcgcg ggaagacggg ctttggcacc
      601 ctgcttggcg ccttaacaag cagggggccg agcgtccgtt ttgtcagcgg acacgtaacc
      661 ggttcccgtg aaggattgct gatccggccc atcctggtcg tccttgagga cggggatcgg
      721 cggatcggca tccagccgct ggtctctgaa tcggccccgg gccgctcagc cccggcgccc
      781 acgccgggcg aaccggaaca ggacccccgg agccccgttc aggccgatcc cctcgaatcg
      841 ttcctgacgc aaatgcggtt agagctttcc gagctgttgc tctccggtgc ccgccgcgcg
      901 ggccagccgc aagcacgagc ctggaccgac ctggcgacgc atggccggga gttgggattt
      961 gtccgtctcg tagagccgct ggcccgcctc gccgagttgc tggcgggccg ggccgacgcg
     1021 ctccgctggg accccgctcc ggcggctcgc gccctcatgg acctctgtgt ttatgcgcga
     1081 atcgccagcg acgcgtagcg cgcctctcaa agccgcgacc agtggtcgcg gtataccagg
     1141 tgcaccgcga ccactggtcg cggctttggg agtcgtccgc cgccgctacg cttgcagtgg
     1201 gcgcggcggc aaccacaata gacgccagcc ctgtcaaccg ccttatcaac tggagaaagc
     1261 tccgtggcca aacgccgcaa caaagccgcc aagattcgcc agatgctggc caagcatccc
     1321 gatgcctcgg ccaaagagat cgttgccgcc ttggccgccc agcggacccg ggtcaccccc
     1381 gcgcaggtct ataacgtcaa gtcgatgcag ggaaaaccga aaaccggccg ctacgacacg
     1441 ctgctgcacg ccaagaagct ggccgacgct ctgggcggca tcgacaaggc ccgccaggcg
     1501 ctcgacctgc tggccaggtt gatgtaggat gcgggttgcc tctaactata cgaaagtggt
     1561 aaactcgcga tggcgagcga ctgcgatcgt gcgttcccct gaaagcagta aatctgcata
     1621 agtgcatatg tacggcagcg tgatttcgat gcaaattact ggtgcgaaac aacttacggc
     1681 aattctgttg gatcgagaaa attgcgccgc atgcatatgc atatgcaggg gaacaaacca
     1741 gggaagccgc ggcgtgctga ctcgcccagg aggcctgcgt ggccttcggt tgaactcggg
     1801 acaagtcaga cggagttaaa cgtgcataaa ctgcatatca tgcacggcga catttcactg
     1861 taagtctcga atttaaaaag gcttaccgct atttgtccaa cgcagcaatt tgttccaggc
     1921 gtgcatatgc accgcatggc tgctgcgcgg cagacaaccg actagccgcg aaacctgcga
     1981 tgtagggcgg ggttacaacc actagcaaac gggcggcaag gcaattcgcc tcttctgccg
     2041 gtttcgacta tgatcgaggt ttgctgatcg cgggtttgca tccaaagtct ttgggcgtca
     2101 acacttgcgt tcgctggccg gggtaaagac gcttcactcc cacgagattt tcatgcgctc
     2161 catcttctct tgctgttgga cggcaggctt gctggttctg gccctggcgg cgccatcctg
     2221 gggtcaggac ccgttcgtcg gccgcatcat cctggtcaac gacgggcaag tcgcgaagga
     2281 tatcggcctg gccgacgccg acgtcgccaa gttgaaggag ttcatcgacc accggctggt
     2341 cgaagcccag accctgatga atgcgaccaa gacggcgccc gccgccgagc gcgaggccaa
     2401 gctggccgag ttcctggccg agtccgaaaa gcagggcctg gagctgctg
//
LOCUS       DASRJB010000493        10968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1828276, whole genome shotgun sequence.
ACCESSION   DASRJB010000493 DASRJB010000000
VERSION     DASRJB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828276"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VFB80_12855"
     CDS             <1..1265
                     /locus_tag="VFB80_12855"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HZN34707.1"
                     /translation="GFLKSEELAEIHQVGAQMDAIRPDLALAETAAKEAMARTDAERA
                     ALKAQKKAEAAERKKQRAEAVARRKASDIVFLGRGVSYGLADRQADIEKLQRQGLPLL
                     STPADVATALELTIPRLRWLAFHSDAAARTHYVRFQVPKKSGGTRELAAPHKTLKKAQ
                     QWILSRILAKLPAHDAAHGFVPGRSTVTGARLHTGKEVVVNLDLKDFFPTITFPRVKG
                     ALAQLGYSPAAATILALLCTESPRRTVTYAGQTLHVATGPRSLPQGACTSPALSNLIA
                     RRLDARLSGLCRKLGWTYTRYADDLTFSAGGETATKVGYLLARVRHIAQDEGFAVNEP
                     KTRVQRRNTRQSVTGLVVNDAVGVPRRTARRLRAILHRARREGLAAQNRSGRPHYLNW
                     IRGMIAYIAMARQAPGRRLRERLGQIVG"
     gene            1351..2352
                     /locus_tag="VFB80_12860"
     CDS             1351..2352
                     /locus_tag="VFB80_12860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34708.1"
                     /translation="MEPGQTIAGRYRIDREIGRGNFGIVYLAEDERTGDLRAIKVLLP
                     WAARNEQLRHRLRREARLAGSLTGEHAVKIHESGETQEGDVFLAMEYLQGEELTHVLQ
                     NRGPLPPARVQEIAQQVLAALDEAHRLGVIHRDLKPSNIFLCRDADGRDFVKVFDFGI
                     AKVTGDGDLMATAKLTVSGGVMGTPVYMSPEQCRGEELTPASDFYSLGIVLYELLAGR
                     VPFDDENPVRIMLMQNQDSVPPLPAAIAASPLGQAVRRSLAKQPGDRFATAGEFQAAL
                     RGEVTRPTPPPAAAEPRPERPAAQSAQPAKSAAWIWWVLAAALLLAAGGVLLFMAWR"
     gene            2444..3379
                     /locus_tag="VFB80_12865"
     CDS             2444..3379
                     /locus_tag="VFB80_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HZN34709.1"
                     /translation="MVEQLRRLTTSEGFNRFILAMIVLSGVLVGCQTYPAFDETTSIG
                     RVVNLVQNIILWIFVGEIVLKIAACGNRPWEYFKRGWNLFDFTIVLICFLPLDARFAT
                     VFRLARLLRTLRMVTILPRLQVLVGALLKSIPSLGYIGILLGLHFYVYACAGTFVFGK
                     NDPIRFGSLHTAMQTLFQVLTLEGWNDVLYTASYGSELQYSDEWKQLAEQKFPGKRLS
                     TPQPAAAAIYFTSFILLGTMIMLNLFTGVIISSMEEAQDEQAEEVREEHMKKKGFLTL
                     HDELNLLSKQLADLSSRIKGMELKEEQREEETADV"
     gene            complement(3376..4491)
                     /gene="holA"
                     /locus_tag="VFB80_12870"
     CDS             complement(3376..4491)
                     /gene="holA"
                     /locus_tag="VFB80_12870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZN34710.1"
                     /translation="MPSALHALDFLAAPAEHKPPAVCVVFGDDPFLKRLVLRSLRRQI
                     VGDDAEVPVASYDCQERRPEWRDVADELATVSLFGGGAPRLVILEGADDFVSAERPKL
                     EDYVAKPRGSGVLVLEVEDWPATTRLYKAVDQSGLAIDCRPPTKGGKSKDVDEGRIVK
                     WIAQWGKSQHNLAIGSDAGQHLFDLTGPVFGLLDANLAKLALLVPPGSKATADQVQEI
                     IGGWRGRSIWALVDSAVGGETAAALAHIDHLLHAGEHPLAIVGSLSWSLRRYASATRI
                     YQQAERAGRKIPLKDALKSAGFRDWPLGELAKAEKRLIALGRHRAGKIYRWLLELDLA
                     LKGSHSSEERGRWALEQLLLRMAKQPAARPQPAHQAS"
     gene            complement(4494..5306)
                     /locus_tag="VFB80_12875"
     CDS             complement(4494..5306)
                     /locus_tag="VFB80_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34711.1"
                     /translation="MTARDKLISDWRARLAAAEAEPVSSSLRRAWLVRVQMRLYRFLL
                     ALYGQGDWRADERPPQKAADVVVFDSAEAESLAGKPAKDLGKIRSVLKTVANSQDHRP
                     AAGSLTSDEVIAGCWFAVAAVSSRLDLIRCIELLRSHKIECRLQFRAGDRIVEVPGPL
                     RTEALQLIDEHRVRLRRRPRIVHQDPQARAVAAPVRFARQPSSPWWAALVFLLACSLL
                     MLVLATAAGNPQPTDEELQARLVASLGTAAVCCVAIFWSWRCSRASPLRHNC"
     gene            complement(5308..5871)
                     /locus_tag="VFB80_12880"
     CDS             complement(5308..5871)
                     /locus_tag="VFB80_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZN34712.1"
                     /translation="MTGQEIVVGVTGGIAAFKTAALVSQLVQGGANVTVVMTAAAEKF
                     VGPSTFAALTGRPVAREIFDESHYPLGAHVMLAEKAKLLVVAPASADFLAKAAQGLAD
                     DLLSTLYLAFSGRVLMAPAMNTAMWEKAAVQRNIAQLRADGVQFIEPESGWLSCRQVG
                     AGRMADPEVILRTIRGLPGDSAAGQNG"
     gene            complement(5916..6176)
                     /gene="rpoZ"
                     /locus_tag="VFB80_12885"
     CDS             complement(5916..6176)
                     /gene="rpoZ"
                     /locus_tag="VFB80_12885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZN34713.1"
                     /translation="MLDALKEESIVNKVGGRFKLSTLIQKRLVQLNAGSRPLVDQSTQ
                     DKMAIVIQEIAQDKIYLDATQQVRVVGEFQGTGEAMELDFGE"
     gene            complement(6161..6754)
                     /gene="gmk"
                     /locus_tag="VFB80_12890"
     CDS             complement(6161..6754)
                     /gene="gmk"
                     /locus_tag="VFB80_12890"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZN34714.1"
                     /translation="MPATATSRLVVISGPSGAGKSTVARELMESCPLPLKWSVSATTR
                     QPRQGEVEGEDYFFLTREQFAARRAAGDFLEWKEYAGNWYGTLQSQVAAGLAEGKWVL
                     LEIDVEGTFAVLERHPDALTIFVNVESFEELERRLRARNTESEEALARRLATAKREIS
                     QKHRYRFEVINREVPNAVREVCEILLKHAKETQCSMP"
     gene            complement(6840..7712)
                     /locus_tag="VFB80_12895"
     CDS             complement(6840..7712)
                     /locus_tag="VFB80_12895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZN34715.1"
                     /translation="MTGHGEAHRHLDGLSIAVEIRTVNNRYFKLNLRVSDGYLALEPH
                     VEAAVRQQIRRGTVQLNVQLAREHSADDFRINTAVLTGYVRQLEGAFGVPLGHGSGSL
                     APLLALPGVVEEPAMALDAIESQWPQVEQVLNEALARLSKMRIEEGRSMAADLAVNAR
                     AMAAELAQVETRAPQVVAAYRTRLTEKLNKLLAEVGSRIEPADVIREVGVFAERGDIS
                     EEIVRLKSHLEQFGTALAAEESQGRKLDFLVQEMFRETNTIGSKANDAEIARHVIEMK
                     TAIERMREMIQNVE"
     gene            complement(7765..8304)
                     /gene="secG"
                     /locus_tag="VFB80_12900"
     CDS             complement(7765..8304)
                     /gene="secG"
                     /locus_tag="VFB80_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZN34716.1"
                     /translation="MSLLLLARVDALHYLFAIPMTVLSIFLVLVILVQRGRGGGLTGA
                     LGGMGGQSAFGTKAGDLFTRITIVVAAIWVLLAMASIKVLAQRTPFDKSQTGYTPPAA
                     KTTPGTTTPGATTPGAKTPGTTSPGATTPGKGAPASGTPAKGATPEAPPPSAAPSSAA
                     PATAPPAEAPPAAPAGKTP"
     gene            complement(8345..9106)
                     /gene="tpiA"
                     /locus_tag="VFB80_12905"
     CDS             complement(8345..9106)
                     /gene="tpiA"
                     /locus_tag="VFB80_12905"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZN34717.1"
                     /translation="MRRKFIAGNWKMNTTRAEGVALAKAIAAKVGASSPVEVAVCPPS
                     LYLEAVGQALAGSAVGLGAQNCYHEAKGAFTGEIAPPMLRDIGCTYVIVGHSERRAIF
                     QESSGDVNKKVHAALGVGLVPIVCVGETLDQRQANQTAAIVRGQIEGSLAGLSAEQML
                     KTVIAYEPVWAIGTGVVATPQQAEEVHADLRRLLETRYNSEVASSIRIQYGGSVNAEN
                     AASLLGQPNIDGALVGGASLKADGFLAIVQAARPA"
     gene            complement(9181..10206)
                     /gene="aroF"
                     /locus_tag="VFB80_12910"
     CDS             complement(9181..10206)
                     /gene="aroF"
                     /locus_tag="VFB80_12910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZN34718.1"
                     /translation="MIIILKSEVTDEQVQHVVERVEALGLKAHLSRGTYRTIIGVIGD
                     EQKISAEPLRAIPGVHDVIPVLPPYKLASREAHPQPSVIDVAGVKIGGGSLAMIAGPC
                     AVEEPERMNAIGAAVKSAGANILRGGAFKPRTSPYAYQGMGEPGLKLLRETGDKFGLP
                     VVTEVMDPRYVEMVERYADMIQIGARNMQNFSLLTEVGRTKKPVLLKRGMSATIEDLL
                     MSAEYILSQGNSQVVLCERGVKGFDKVTRNLFDVAAVAVVKGLSHLPIIVDPSHATGR
                     PDLIPACALAGVAAGADGVHIEVHDCPERAKSDGPQALLPRQYSEVMGQIRKLAALFG
                     KTISPSK"
     gene            10450..>10968
                     /locus_tag="VFB80_12915"
     CDS             10450..>10968
                     /locus_tag="VFB80_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZN34719.1"
                     /translation="MFLAFGEIMARIAPPGALRWRQALPGAVNVTWGGGEANVCASLA
                     LLGQKARFLTALPKTPVTEALVGTLRGLGVDTEQILWRKEGRLGLYFVEGGANQRGST
                     VLYDRGQSAVSLAGPEEYDFARVLAGVKWLHVTGITPAIGEAACRANLALVKQAKAAG
                     TRVSCDLNFRKKL"
BASE COUNT         1773 a         3700 c         3629 g         1866 t
ORIGIN      
        1 acggctttct gaagagcgag gagctggccg aaatccacca ggtcggcgcg cagatggacg
       61 cgatccggcc ggacctcgcc ctggccgaga cggccgcgaa agaggcaatg gcccgcaccg
      121 acgccgagcg ggccgccctc aaagcccaga aaaaagccga agccgccgag cgaaagaagc
      181 agcgggccga agcggtcgcc cgccgcaagg ccagcgacat cgtgtttctc ggccgcggcg
      241 tctcgtacgg cctggcagac cggcaggccg acatcgaaaa gctgcagcgg cagggattgc
      301 ccctgctctc cacgccggcc gacgtcgcga ccgccctgga gctgacgatt ccgcggctgc
      361 ggtggctggc cttccacagc gacgccgccg cccgcacgca ctacgtccgc tttcaagtgc
      421 ccaaaaagag cggcggcacg cgggagctgg ccgcgccgca caagacgctg aagaaagccc
      481 agcagtggat cttgagccgc atcctggcaa agctgccggc ccacgacgcg gcccacggct
      541 tcgtgccggg ccgcagcacc gtgaccggcg ctcggctgca cacgggcaag gaggtggtcg
      601 tcaacctgga cttgaaggac tttttcccga cgatcacgtt cccgcgggtc aaaggcgcac
      661 ttgcgcagct cggctactcg cccgcggcag cgacgatcct ggcactgctg tgcaccgagt
      721 cgccacggcg gacggtaacg tacgcgggcc agacgctgca cgtcgccacc ggcccgcgct
      781 cgctgccgca aggcgcgtgc accagcccgg cgctctcgaa cctgatcgcc cggcggctcg
      841 acgcgcggct ctccggcctg tgccgcaagc tcggctggac ctatacccgc tatgccgacg
      901 acttgacgtt ctcggcgggc ggcgaaacgg caacgaaggt cggatacctg ctggcccgcg
      961 tgcggcacat cgcccaggac gagggctttg ccgtaaacga gccgaagacg cgcgtgcagc
     1021 ggcggaatac gcggcagagc gtcacggggc tggtggtcaa cgacgcggtc ggcgtgccgc
     1081 ggaggacggc ccgccgcctg cgggcgattc tgcaccgcgc tcgccgcgag ggactggcgg
     1141 ctcagaaccg cagcggccgc ccgcactacc tcaactggat tcgcggcatg atcgcctaca
     1201 tcgccatggc ccgccaggcg ccgggacgcc ggctccgcga gaggctgggc cagatcgtcg
     1261 gctgattgtc tcacgcggag cgtgagggcg actttgcgat acaatatcgc cggggacagg
     1321 gaacgactcg cgcaccaacc gtcgcgtcgg atggaaccgg ggcagaccat cgccggccgt
     1381 tatcggatcg atcgagagat cggccgcggc aactttggca tcgtctatct ggccgaggac
     1441 gagcggaccg gcgacttgcg ggcgatcaag gtgctgctgc cctgggccgc ccgcaacgag
     1501 cagctgcggc accgcctccg ccgcgaagcc cgcctggcgg gcagcctcac cggcgaacac
     1561 gccgtgaaga tccacgaatc gggcgagacg caggaaggcg acgtctttct ggcgatggag
     1621 tacctgcagg gggaggagct cacgcacgtc ctgcagaatc gcggtccctt gccgccggcg
     1681 cgggtgcaag agatcgccca gcaggtgctc gcggctctcg acgaggccca ccgcctgggc
     1741 gtcatccacc gcgacctcaa gccgagcaac atcttcctct gccgcgacgc ggacggccgc
     1801 gatttcgtca aggtcttcga cttcggcatc gccaaggtga ccggcgacgg cgatttgatg
     1861 gcgacggcga aactgacggt ctcgggcggc gtgatgggca cgcccgtcta tatgagcccc
     1921 gagcagtgcc gcggcgaaga gctgacgccg gcgtcggact tctattcgct gggaatcgtc
     1981 ctctacgagc tgctcgcggg ccgcgtgccc ttcgacgacg agaacccggt ccgcatcatg
     2041 ttgatgcaga atcaggactc ggttccgccg ctgcccgccg ccatcgcagc ctcgccgctc
     2101 ggtcaggccg tcaggcggtc gctcgccaag cagccgggcg atcgatttgc cactgccggc
     2161 gagtttcagg cggcgctgcg cggcgaagtt accaggccga cgcctccgcc ggcagccgcc
     2221 gagccgcggc cggagcgccc cgcggcacaa tcggcccagc cggcgaaatc ggcagcctgg
     2281 atttggtggg tgctggccgc ggctctcctg ttggccgccg gcggcgtgct acttttcatg
     2341 gcttggcggt agcggaccgt ccgttgtcgc ccgtcgccaa tcgcagtatt atcggaacgc
     2401 gcgccggccg agacgtgcac agggccccca gcggaggatt gtcgtggtcg aacaactaag
     2461 gcgacttacc acctcggagg ggttcaatcg cttcattctg gcgatgattg tgctctcggg
     2521 tgttctcgtc ggctgtcaga cctaccccgc gttcgacgaa acgacgtcca tcggccgggt
     2581 cgtcaacctg gtgcagaaca tcattctctg gatcttcgtc ggcgagatcg tcctgaagat
     2641 cgccgcctgc ggcaatcggc cgtgggaata cttcaagcgc ggctggaacc tgtttgactt
     2701 cactatcgtc ctcatctgct tcctgccgct ggatgcccgg tttgcgacgg tctttcgcct
     2761 ggcccggctg ctacgcaccc tgcgcatggt gacgatcttg ccgcggctgc aagtgctggt
     2821 gggagcgctc ttgaaatcga ttccctccct gggctacatc ggcatcctgc tgggcctgca
     2881 tttctacgtc tacgcctgcg ccgggacgtt cgtgttcggc aagaatgacc cgatccgctt
     2941 cggcagcctg cacaccgcga tgcagacgct gtttcaggtg ttgacgctgg aaggttggaa
     3001 cgatgttctg tacacggcgt cctacggctc agaattgcag tattcggacg aatggaagca
     3061 gctcgccgag caaaaatttc ccggcaagcg cctgtcgact ccgcagccgg cggcggcggc
     3121 gatctacttc acgtcgttca ttctgctggg gacgatgatc atgctgaacc tgttcacggg
     3181 cgtcatcatc agcagcatgg aggaggccca ggacgagcaa gctgaagagg tccgcgagga
     3241 gcacatgaaa aagaaaggct ttctcacgtt acacgacgag ctgaacctgc tgagcaagca
     3301 gctcgccgac ctctcgtcgc ggatcaaggg aatggagttg aaggaagagc agcgcgagga
     3361 ggagaccgcg gacgtctagc tcgcctggtg ggcgggctgg ggccgggccg ccggctgttt
     3421 ggccatccgc agcagaagct gctccagggc ccagcggccg cgttcttccg acgagtggct
     3481 gcctttgagg gccaggtcga gctccagcag ccagcggtaa atcttgccgg cccggtggcg
     3541 gcccagcgcg atcaggcgtt tctcggcttt ggccagctcg cccagcggcc aatcgcgaaa
     3601 gccggcgctt ttgagggcgt ccttgagcgg aatcttgcgg ccggcccgct cggcctgctg
     3661 atagattcgc gtggccgagg cataccgccg cagcgaccag gagagcgagc cgacgatcgc
     3721 cagcggatgc tcgccggcgt gcagcaggtg gtcgatgtgc gccagagcgg ccgccgtctc
     3781 acctccgacg gcgctatcaa ccagcgccca aatgctgcgg ccgcgccagc ctccgatgat
     3841 ctcctggacc tggtcggccg tcgccttcga tcccggtggc acgagcaggg cgagcttggc
     3901 caggttggcg tcgagcaggc cgaacaccgg cccggtcaga tcgaacaggt gctgcccggc
     3961 gtcgctgccg atggcgagat tgtgctgcga cttgccccac tgggcgatcc acttcacgat
     4021 ccgtccttca tcgacgtcct tgctttttcc gcccttggtg ggcgggcggc agtcgattgc
     4081 caggccggat tgatcgacgg ctttgtagag ccgcgtcgtc gccggccagt cttcgacttc
     4141 caggacgagc acgccgctgc cgcgcggctt ggcgacgtaa tcttccagct tcggccgctc
     4201 cgccgagacg aaatcgtccg ccccttccag gatcaccagc cttggagccc cgccgccgaa
     4261 cagcgacacc gtcgccagct cgtccgccac gtcgcgccat tccggccgcc gctcctggca
     4321 gtcgtagctg gccaccggca cttccgcatc gtcaccaacg atctgccgcc gcagagatcg
     4381 cagcaccagc cgcttcaaga acggatcgtc gccaaagacc acgcacaccg ccggcggctt
     4441 gtgctccgcc ggcgcggcga ggaaatcgag ggcgtggagg gcgctgggca tcgtcagcaa
     4501 ttgtgccgca gcgggctcgc ccggctgcac cgccaggacc agaagatggc gacgcagcaa
     4561 acggccgcgg tgcccagcga ggccaccaat cgagcctgga gctcctcgtc cgtgggctgc
     4621 ggattgccgg ctgccgtagc cagcacgagc atcagcaggc tgcaggcgag caggaacacc
     4681 agcgccgccc accacggcga gctgggctga cgggcaaagc gaacgggcgc tgcgaccgcc
     4741 cgtgcctgcg ggtcctggtg aacaatccgc ggccttctcc gcaatcgcac gcggtgctcg
     4801 tcgatcagct gcagcgcctc ggtgcgcagc gggccgggta cctcgacgat ccggtcgccc
     4861 gcgcgaaatt gcaggcggca ctcgatcttg tggctgcgga gtaattcgat gcagcgtatc
     4921 aggtccagac gcgagctgac cgcggccacg gcgaaccagc agccggcgat aacctcatcg
     4981 gaggtcagcg agccggcggc cggacggtgg tcctggctgt tggcgaccgt cttgagcacc
     5041 gagcggattt tgcccaggtc cttcgcgggc ttgcccgcga gcgattcggc ctcggcagag
     5101 tcgaagacga cgacgtcagc cgctttctgc ggcgggcgtt cgtcggcccg ccagtcgccc
     5161 tggccgtaga gcgcgagcaa aaagcggtac agccgcatct gcacgcggac cagccaggcc
     5221 cggcgaagcg acgagctgac cggctccgcc tcggccgcgg ccaggcgggc ccgccaatcg
     5281 gagatcaact tgtcgcgggc ggtcatgtca accattctgt cccgccgcac tgtcgccagg
     5341 caagccgcga atcgtccgca ggatgacttc cgggtcggcc atccggcccg cgccgacctg
     5401 gcggcagctc agccagccgg attcgggctc gatgaattgc acgccgtcgg cgcggagctg
     5461 ggcaatgttc cgctgcacgg cagctttctc ccacatcgcc gtgttcatcg ccggggccat
     5521 gagcacgcgg ccggaaaacg ccaggtagag cgtcgagagc aggtcgtcgg ccaggccctg
     5581 cgcggccttg gccagaaagt cagccgaagc cggcgcgacg acgagcagct tcgccttctc
     5641 ggccagcatg acgtgcgcgc cgagcggata gtgcgattca tcaaagattt cgcgggcgac
     5701 cgggcggccg gtgagggccg cgaacgtcga ggggccgacg aatttctcgg ccgcggccgt
     5761 catcacgacc gtgacgttcg ccccgccctg cacgagctgg ctgaccaggg ccgccgtctt
     5821 gaacgcggcg atgccgccgg tcacgccgac gacgatttct tggccggtca tgcagaatca
     5881 tcggaaggct tacgcctccc gctcgctggc ggccgtcact cgccgaagtc cagctccatc
     5941 gcctcgccgg tgccctggaa ctcgccgacg acgcggacct gctgcgtggc gtcgaggtaa
     6001 atcttgtcct gggcgatctc ctgaatcacg atcgccatct tgtcttgcgt gctctgatcg
     6061 acgagcggtc ggctgcccgc gttgagttgc accagccgct tctgaatcag cgtgctgagc
     6121 ttgaaccggc cgccgacctt gttgacgatc gactcttcct tcagggcatc gagcattgcg
     6181 tctccttggc gtgcttcagc agaatctcgc aaacttcgcg aacggcgttt ggcacttcgc
     6241 ggttaatgac ttcgaaccgg tagcggtgct tctggctgat ttcccgtttg gccgtcgcca
     6301 ggcggcgagc cagggcttct tcgctttcgg tgttacgggc acgcagtcgc cgctccaact
     6361 cttcgaacga ctcgacgttg acgaagatgg tcagcgcgtc gggatgccgc tccaggacgg
     6421 cgaaggtgcc ctcgacgtca atctccaaga gtacccattt tccttccgca agtccagcgg
     6481 cgacttggct ttgcagcgtg ccgtaccaat tgccggcgta ctccttccac tccaggaagt
     6541 cgccagccgc acgccgagcg gcaaactgtt cccttgtaag aaagaagtaa tcttccccct
     6601 cgacctctcc ctgccgcggc tgccgggtcg ttgccgagac gctccacttc agcggcagcg
     6661 ggcagctttc catcagctcg cgcgcgacgg tcgatttccc cgcgccggaa gggccggaga
     6721 tgacgacgag gcggctggtg gcagtagcag gcataggctc aaagaaccaa aggaccgaag
     6781 aaccaaagca gccttatttg cccgccttcg gttctttggt tcttcggttc ttgggatttc
     6841 tactcgacat tctgaatcat ttcccgcatc cgctcgatgg ccgtcttcat ctcgatcaca
     6901 tgccgggcga tttcggcgtc gttggccttg gagccgatgg tgttggtctc gcggaacatt
     6961 tcttgcacca ggaagtctag cttgcggcct tgcgactctt ccgccgcgag ggccgtcccg
     7021 aactgctcca ggtggctctt caggcggacg atttcttcgg aaatgtcccc ccgctcggca
     7081 aacacgccga cttcgcggat cacgtcggcc ggctcgatcc ggctgccgac ctcggccagc
     7141 agcttgttga gcttctcggt cagccgcgtg cgataggcgg cgacgacctg cggggcgcgg
     7201 gtctcgacct gggccaactc ggcggccatg gcccgggcgt tgacagccag gtcggccgcc
     7261 atggagcggc cttcctcgat ccgcatcttg gacagccggg cgagggcctc gttcaaaacc
     7321 tgctcgacct gcggccactg cgattcgatc gcgtccagcg ccatggccgg ctcctcgacg
     7381 acgcccggca gtgccagcag cggcgcgagg ctgccgctgc cgtggcccag cggcacgccg
     7441 aacgcccctt cgagctgccg gacatagccg gtgaggaccg ccgtgttgat gcggaagtcg
     7501 tcggcggagt gctcgcgggc aagctgcacg ttcagctgca cggtgccgcg gcgaatctgc
     7561 tgccggacgg cggcctcgac gtgcggctcc agggccaggt agccgtcgct gacgcggaga
     7621 ttcagcttga agtagcggtt gttgaccgtg cggatttcga ccgcgatgga caagccgtcc
     7681 aggtgccggt gggcctctcc gtggccggtc atgctcagca gcaacttcgg cggctcctgg
     7741 gcttgcactg caagcggttg gatctcacgg cgtcttgccc gcgggcgctg cgggaggcgc
     7801 ttcagcaggc ggcgctgtgg ccggcgcggc gctagacggt gcggcagagg gaggaggcgc
     7861 ttcgggagtc gcaccctttg ccggtgtgcc gctggccgga gcacccttgc ccggcgtcgt
     7921 ggcgccaggc gaggttgtgc ccggcgtctt ggcacctggc gtcgtggcac ctggcgtcgt
     7981 cgtgcctggc gtcgtcttag ccgcgggcgg cgtgtatcca gtttggctct tgtcgaacgg
     8041 cgttcgctgg gcgagcactt tgatcgaggc catcgccagc agcacccaga tcgccgccac
     8101 gacgatcgtg atccgcgtga acaagtcgcc ggccttggtg ccgaacgcgc tctggccgcc
     8161 catcccgccc agagcgccgg tcaagccgcc gccgcggccg cgctggacca ggatcaccag
     8221 gaccaggaag atcgacagca cggtcatcgg aatcgcaaac aggtaatgca gcgcgtccac
     8281 gcgggccaaa agcagtaagc tcataggttc taatcccttc ccaattcaac catgcggcga
     8341 atgctcaggc aggcctggca gcttggacga tcgccagaaa gccgtccgcc ttgagcgacg
     8401 cgccgccgac cagtgcgccg tcgatgttgg gctgccccag caggctggcg gcgttctcgg
     8461 cgttgacgct gccgccgtac tggatccgga tggacgacgc aacttctgaa ttgtagcggg
     8521 tttccagcaa cctgcgaagg tcagcatgca cctcctccgc ctgctgcggc gtcgcgacca
     8581 cgccggtgcc gatcgcccag actggctcgt aagcgatgac ggttttcagc atctgctcgg
     8641 ccgaaagccc ggccagcgag ccctcgatct gcccgcgcac gatggcggct gtctggttgg
     8701 cttgccgctg gtcgagcgtt tcgccgacgc agacgatcgg tacgaggccg acgcccaggg
     8761 ccgcgtggac cttcttgttc acatcgccgc tggattcctg gaagatcgcc cgccgttcgc
     8821 tgtggccgac gatcacatac gtgcagccga tgtcgcggag catcggtggg gcgatttcac
     8881 cggtgaaggc gcccttggcc tcgtgatagc agttctgcgc gcccaggccg acggctgaac
     8941 cggccagcgc ctggccgacg gcctcgagat acaagctcgg cgggcaaacg gcgacttcca
     9001 ccggcgaaga cgcgccgacc ttcgcggcga tcgccttcgc gagcgccacg ccttcggccc
     9061 gcgtggtgtt cattttccaa ttgccggcga tgaacttgcg tcgcataggc acctatagta
     9121 gtcctcacgc tccgcgtgag gaaaccgcat ctcgcggagc gagatgcctg cttgtatcgg
     9181 ctactttgac ggcgaaatcg tcttcccgaa cagcgcggcc agcttccgga tctgccccat
     9241 cacctcgctg tattgccgcg gcagcagggc ctgcgggccg tcggacttgg cccgctcggg
     9301 gcagtcgtgg acctcgatgt gcacgccgtc cgcgcccgct gccacgcccg ccagggcgca
     9361 ggccgggatc aggtcgggcc ggccggtcgc gtggctcgga tcgacgatga tcggcaggtg
     9421 cgacagcccc ttcacgactg ccaccgccgc cacgtcgaac aggttccgcg tgaccttgtc
     9481 gaaacccttc acgccgcgct cgcacaggac gacttgcgag ttgccctgcg acaagatgta
     9541 ttcggcgctc atcagcaaat cttcgatcgt ggcgctcatg ccacgcttga gcagcaccgg
     9601 cttcttggtg cggccgacct ccgtcagcag gctgaagttc tgcatattgc gggcgccgat
     9661 ctggatcatg tcggcatacc gctcgaccat ctcgacgtac cgcggatcca tcacttcggt
     9721 gacgaccggc aggccgaact tgtcccccgt ctcgcgcagc agcttcagcc ccggctctcc
     9781 catgccctga taggcgtagg ggctggtccg cggcttgaat gccccgccgc ggaggatgtt
     9841 cgccccggcg gatttgaccg ccgccccgat ggcgttcatc cgctccggct cctcgaccgc
     9901 acagggccct gcgatcatcg ccaggctgcc gccgccgatc ttcacgcccg ccacgtcgat
     9961 cacgctgggc tgtggatggg cttcgcggct ggccagcttg tacgggggca gcaccgggat
    10021 cacgtcgtgc acgccgggaa tcgcccgcag cggctcggcg ctgatcttct gctcgtcgcc
    10081 gatcacgccg atgatcgtgc gataggtgcc gcggctgagg tgagccttca ggcccagcgc
    10141 ctcgacgcgc tcgacgacgt gctggacctg ttcgtcggtg acttcggatt tgaggatgat
    10201 gatcatggga gtcgcctcac tggaaacgcg gcccaacgaa ttgcggaccg caggcaactc
    10261 tgcctaagcg caatccgcag cccggcgcta caccgtgagg ttacctggcc ctagtggaat
    10321 ctcggatcat agcatagccg cgaagtcgtt gtcagggttc gcgcgccaaa cgcggtaggg
    10381 cggctgctgc ttgccggctg ccggggcgaa gtctaagttg ggcgccaagc cttctcggac
    10441 gagacgagca tgttcctggc attcggcgaa atcatggcgc ggattgctcc gccaggggcg
    10501 ctgcgctggc ggcaggcgct gccgggggcg gtgaacgtaa cctggggcgg cggcgaggcc
    10561 aacgtctgcg cgtcgctggc gctgctcggg cagaaagccc gctttctgac cgcgctgccg
    10621 aagacgcccg tcaccgaagc gctggtcggc acgctccgcg ggctgggcgt cgataccgag
    10681 cagattctct ggcggaagga gggtcggctg ggactctatt tcgtcgaagg cggcgcgaat
    10741 caacgcggat cgacggtgct ctacgaccgc gggcagagcg cggtcagcct ggccgggccc
    10801 gaggaatatg acttcgcgcg ggtgctggcg ggcgtgaagt ggctgcacgt cacgggcatc
    10861 acgccggcga tcggtgaggc ggcctgccgg gccaacctgg ccctcgtcaa gcaggccaag
    10921 gccgccggca cgcgcgtctc gtgcgacctc aactttcgca agaagctc
//
LOCUS       DASRJB010000494         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_987205, whole genome shotgun sequence.
ACCESSION   DASRJB010000494 DASRJB010000000
VERSION     DASRJB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987205"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFB80_12920"
     CDS             <1..778
                     /locus_tag="VFB80_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZN34720.1"
                     /translation="LWKELKIVAFDPALAAAMGFRVALVHYLLMGMVSLVTVASFESV
                     GSILVVAVLIVPAATAALITERLSWMIAWAVAIGATSAVFGYTAAMRLNTNVAGATAV
                     AAGLQFTAAVFLSPQRGLVSRWLRNQALAVRIAAEDVVGRLYRAEESGARGQEPGASG
                     VVGWLANWRLFQRGWVMRDRAGDLRLTAEGRDAARRLIRAHRLWESYLDTHFDLPRDH
                     LHEAAERMEHYLDPQLQDELAAELAGRAVDPHGKEIPSAP"
     gene            949..2382
                     /locus_tag="VFB80_12925"
     CDS             949..2382
                     /locus_tag="VFB80_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN34721.1"
                     /translation="MHWLCQPRSFAVVAAWLLSSLLSAAIACGQTIGDEAPEFWPTQP
                     APLAAAEPAAYHTTTEMESVLRRLDALETQTAKSVQPAPAAPKPADGWIDVSKEKWTV
                     RLGGHVQLDLINWAQASPAIVGDQNYFEFRRLRLSAEGTGYGVLDFRLQLTLEPESVG
                     DISTPQTPEVKDAYLSMNEVPFLGRFRIGNFFVPFGLEQVTNDRFNIFLERSIPTQGI
                     FTADREVGMAFYNCTEDQRLTWCGGVFLDSISEALKERIDDNQGCRVSGRVTWLPYYN
                     EPSNGRYLIHTGLGILYTDDHDNRVRFRARPQIHEGPFLINSGILNATSNTTGNVELA
                     IVAGPVTVQSEAFVSSVNLIGAGAVNVQGAYAHLSYFLTGENRNYERFGQHGAQFAGI
                     TPFSNFFWVPGSFSPGAWEFRTRWSLLDLNQVNAGQYNDLTVGFNWYWNERVRVMFDW
                     IHPITSSQTVFGATQSDILGMRFDFAW"
     gene            2419..2673
                     /locus_tag="VFB80_12930"
     CDS             2419..2673
                     /locus_tag="VFB80_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34722.1"
                     /translation="MSEPQEKRGCLPLALSAVVICLALYVPSCGPVLATAFWLREATG
                     WDGFYAVMWLYYPLLVWGHGGLLGQYIEWWVKLFGTVGPG"
     gene            2686..4413
                     /locus_tag="VFB80_12935"
     CDS             2686..4413
                     /locus_tag="VFB80_12935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN34723.1"
                     /translation="MDSRNRSRRTFLWQGALTAGGVACLPVLGRAQEPPVEVDGGPDL
                     VVINAKIYTQEERQPRAEALAVTGDRLLAVGSTERIKALATKRTRVIDAEGMTLVPGF
                     IDAHTHPADSGVSELMFVDCNRPAVAEIQAAMRGRAAKTPAGEWVAGFKFDDTKLKDG
                     PLTRTHLDAAAPDHPARVLHRGGHTAIVNSAAFKQLGITKDTPDPEGGKFGRGSDGEL
                     TGFVAETALKVVDRGTRTPAVGPRERQAGVKLISEMMTAAGLTTVHDAQASKDDFQAY
                     QDALAAGQMLFRTYLLVVPELLADLQRAGVRARMGSDRLRIGGVKLFCDGSASERTMR
                     MSRPYVGRPNDYGILVTTQDKLNDAVLAAHQAGFQVGVHANGDVAIDMVLTAYELAQR
                     LAPREGTRHRIEHCTLVNPELLKRIAAAGVIPTPFYTYVYYHGDKWAQYGDERVRWMF
                     AHRSFLDHGIRVAGASDYVPGPFEPLMAIQSMVTRTDYAGRVWGENQRISVAEALQIC
                     TLHGAYASFEERHKGSLAAGKLADFVLLEGDPHEVDPGRIKDIRVARTVVGGQTVHPA
                     AESGGRQTR"
BASE COUNT          818 a         1562 c         1655 g          826 t
ORIGIN      
        1 cctctggaag gagctaaaga tcgtcgcgtt cgatccggcg ctggcggcgg cgatgggctt
       61 tcgcgtcgcg ctggtccact acctgctgat gggcatggtc tcgctcgtca cggtggcgtc
      121 gttcgagtcg gtcggctcga tcctggtcgt cgccgtgctg atcgtccccg ccgcgaccgc
      181 cgccctgatc accgagcgat tgagctggat gatcgcctgg gccgtcgcca tcggggccac
      241 gtccgcggtc ttcggctaca cagccgccat gcggctgaac accaacgtcg ccggcgcgac
      301 cgccgtcgcc gcgggcctgc agttcaccgc cgccgtcttc ctttcgccgc agcgggggct
      361 cgtcagccgc tggctgcgga accaggcgct ggcggtgcgg atcgcggcgg aggacgtggt
      421 ggggaggctg tatcgggcgg aggagtcagg ggccaggggc caggagccgg gagccagcgg
      481 tgtggttggt tggctggcga attggcggct ctttcaacgc gggtgggtga tgcgcgatcg
      541 cgcgggcgac ttgcggctga cggcggaggg gcgcgacgcc gcgcggcggc tgatccgtgc
      601 gcaccgcctc tgggagagtt acctcgacac gcacttcgac ttgccgcgcg accacctgca
      661 cgaagcggcc gagcggatgg agcactacct cgatccgcag ctccaggacg agctggccgc
      721 cgagctcgcc ggccgcgcgg tagatccaca cgggaaagaa atcccctcgg ctccttagcc
      781 gggaatcgct caagctgctg gcacccgcgt cccgatactt tttcaatctg tatcggttgt
      841 aacgattctg ccgattacgc cgattgccat cacccaaacg cgcgacttta ctccgaccgt
      901 tgactggaac caccagaggc agccggtgat gtcaggcggg aacctccgat gcattggctt
      961 tgccagccgc gatctttcgc tgtggtagcg gcctggctgc tgtcgtcact gctatccgcg
     1021 gcaattgcgt gcgggcagac gatcggcgat gaggcgcctg aattctggcc gacacagcct
     1081 gcgccgctag cggcggcaga gccggccgcg tatcacacca ccaccgagat ggagtctgtc
     1141 ctccgccgcc tcgatgcgct ggaaacgcaa accgccaaat ctgtgcagcc tgcgcccgcc
     1201 gcccctaagc cggccgacgg ctggatcgac gtctcgaaag agaagtggac cgtgcggctg
     1261 ggcgggcacg tgcagctcga ccttatcaac tgggcccaag ccagcccggc gatcgtcggc
     1321 gaccaaaact attttgagtt ccgccgcctg cggctctcgg ccgaggggac gggttacggc
     1381 gtgctggatt ttcgcctgca gctgacgctg gagcctgagt cggtgggcga catcagcacc
     1441 ccgcagacgc cggaagtcaa agacgcctat ctgtcgatga acgaggttcc gttcctgggg
     1501 cggtttcgca tcggcaactt cttcgtcccc ttcggcctgg agcaagtgac gaacgaccgg
     1561 ttcaacatct tcctggaacg atcgattcct acgcagggca tcttcaccgc cgaccgcgaa
     1621 gtgggaatgg cgttttacaa ctgcaccgag gaccagcggc tgacctggtg cggcggcgtt
     1681 ttcctcgaca gcatcagcga ggcccttaag gagcggatcg acgacaatca gggctgccgc
     1741 gtgtcgggcc gcgtgacctg gctgccgtat tacaacgagc ccagcaacgg gcggtatttg
     1801 atccacaccg gcctgggcat tctctacacc gacgaccacg acaatcgggt tcgcttccgt
     1861 gcccgcccgc agattcacga gggtccgttc ctgatcaaca gcggcatttt gaacgccacg
     1921 tcgaatacca ccgggaatgt cgagctggcg atcgtggcgg ggccagtgac ggtgcagagc
     1981 gaggcattcg tttccagcgt gaatctcatc ggcgcgggcg cggtgaacgt gcaaggggcg
     2041 tacgcccacc tgagctattt cctgacgggt gagaaccgca actacgaacg gtttggccag
     2101 cacggggcgc aattcgccgg tatcacaccg ttctcgaact tcttctgggt gcccggaagt
     2161 ttttcgcccg gggcgtggga gttcagaacg cggtggagcc tgctggacct gaaccaggtc
     2221 aacgccggcc agtacaacga cttgaccgtc ggcttcaact ggtattggaa cgagcgcgtc
     2281 cgcgtgatgt tcgactggat tcatcccatc accagctcgc agaccgtgtt cggcgcgacg
     2341 cagtcggaca ttctggggat gcggttcgac tttgcctggt agctacggag acactttgac
     2401 caacggtaga atgcggcgat gagcgagccg caggaaaaac gcggttgctt gccgctggca
     2461 ctgtctgccg tggtgatttg cctcgcgctc tacgttccca gctgcggacc ggtgctggcc
     2521 acggcgttct ggctgcgcga ggccacgggc tgggacggct tctacgcggt gatgtggctc
     2581 tactatccgc tcttggtctg ggggcatggc ggactgctcg gccagtacat cgaatggtgg
     2641 gtcaagctgt ttggcaccgt cggccccggc taaaggacca gagcgatgga ttcacgcaac
     2701 cgctcgcggc ggacgttctt atggcaaggc gcgctcactg ccggcggcgt cgcttgcctg
     2761 ccggttttgg gccgggcgca ggagccgccg gtcgaggtcg atggcgggcc cgacctggtc
     2821 gtgatcaacg ccaagatcta cacgcaggag gagcggcagc cgcgggctga ggcgctcgca
     2881 gtgaccggcg accgtctgct ggcagtgggg agcacggagc gaattaaggc gctggcgacg
     2941 aagcggacgc gggtgatcga tgcggaagga atgacgctgg tgccggggtt catcgacgcc
     3001 cacacgcacc cggccgattc cggcgtgagc gagctgatgt tcgtcgattg caaccggccc
     3061 gcggtggccg aaatccaagc ggccatgcgc gggcgggcgg ccaagacgcc ggccggcgaa
     3121 tgggtggcgg gattcaagtt cgacgacacg aagctcaagg acgggccgtt gacgcgcacg
     3181 cacctcgacg cggccgcgcc ggaccatccg gcccgcgtgc tgcatcgcgg cgggcacacg
     3241 gcgatcgtca atagcgcggc tttcaagcag ctcggcatta ccaaggacac gcccgatccc
     3301 gagggcggca agttcggcag aggcagcgat ggcgaattga ccggcttcgt ggccgagacg
     3361 gccctcaagg tcgtcgatcg cggcacgcgc acgcccgcgg tcggcccgcg cgagcggcag
     3421 gcgggcgtga agctgatctc cgagatgatg accgccgccg gcctgacgac ggtgcacgac
     3481 gcgcaggcga gcaaggacga ctttcaggcc tatcaggacg cgctggccgc cgggcagatg
     3541 ttgtttcgga cctacttgct ggtcgtgccc gaactgctgg ccgatttgca gcgggcgggc
     3601 gtccgcgcgc gaatgggcag cgatcgcctg cggatcggcg gcgtaaagct gttttgcgac
     3661 ggctcggcct cggagcggac gatgcggatg agccggccct acgtcggccg gcccaacgac
     3721 tacggcatcc tcgtcacgac gcaggacaag ctgaacgacg cggtgctcgc cgcgcaccag
     3781 gccggctttc aggtcggcgt ccacgccaac ggcgacgtgg cgatcgacat ggtgctcacc
     3841 gcctacgagc tggcccagcg gctcgcgccg cgcgaaggca cgcggcaccg catcgagcac
     3901 tgcacgctgg tcaaccccga gctgctcaag cggatcgccg cggccggcgt catcccgacg
     3961 ccgttttaca cctacgttta ctaccacggc gacaagtggg cccagtacgg cgacgagcgg
     4021 gtgcggtgga tgttcgccca ccgctcgttt ctcgaccacg gcatccgcgt cgccggagca
     4081 tcggactatg tgcccggccc gttcgagccg ctgatggcga tccagagcat ggtcacgcgc
     4141 accgactatg ccggccgcgt ctggggcgaa aaccagcgga tcagcgtcgc cgaggccctc
     4201 cagatctgca cgctgcacgg cgcttacgcc agctttgagg agcgccacaa gggctcgctc
     4261 gccgccggca agctggccga cttcgtgctg ctggaaggcg atccacacga ggtcgatccc
     4321 ggccgcatca aagacatccg cgtcgcccgt acggtcgtcg gcggccagac ggtgcacccc
     4381 gccgcggaat cgggcggccg gcagacgcgg tgacggcggc tcgcaactag actgggcaac
     4441 tctagactgg gcaacttgaa gtcagccgca acccagaggc cgccatgatt cgccgctcgc
     4501 tgttgctcct cttgttcatc gctgcgcccg ccgcggctga gtctcctgcg ccggatctgc
     4561 cgcctggcgc gaggccgtag agggccgcgg gggcaaaggt attcgccact tggagagccc
     4621 ggcgacaacg ccgggccgtt tcgcgcggaa cgattgccgc gcatgctcgg tggtgaggtt
     4681 gctgtccagc agcagcagtt tgagcctggg caatccgctc agggcctcca acgaagtgtc
     4741 cgtcagcgga attccggaaa gatcgacgtg ctggagattt ggatgacctt ggaaaggaca
     4801 aatcccgtcg tcggagatcg ttctcgtgcg ggcgagaccc aagccctcca atctcgggca
     4861 g
//
LOCUS       DASRJB010000495         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3238111, whole genome shotgun sequence.
ACCESSION   DASRJB010000495 DASRJB010000000
VERSION     DASRJB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238111"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..884
                     /locus_tag="VFB80_12940"
     CDS             150..884
                     /locus_tag="VFB80_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34724.1"
                     /translation="MIRPLACLMVAAGSLLLLADDAPPAKNKEHAAQQERLEKIQQLV
                     GAWRGVAQPQRGSTRDTWIEEADWAWSFGKEGPALVAKSPKAKFFSQLSLTTGKDAGQ
                     YVLAATPAAGGEAVRYAGTLDDEQRLVLTLEKPQGDLPQRLSFRFVAGGDRLLVLMEK
                     KSPTSDQLVRLAEVGYTRQGSGFGKNTAQRECVVTGGLGTIEVMHKGQTYYVCCTGCR
                     DYFYEKPDEILAEYVARKAAEKAKRP"
     gene            1090..1785
                     /locus_tag="VFB80_12945"
     CDS             1090..1785
                     /locus_tag="VFB80_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZN34725.1"
                     /translation="MKRLLLASLVLLVANQASAALIARWTFETSPPADLNNSATIGGI
                     LADAGIGTASGVHASAATDWSTPVGNGSGNSLSSNEWAVGDYYQFAFNAAGLTNLQLN
                     FDQTSSNTGPRDFKVQTTLDGTLFTDLVNYVVLANAAPNPFWTSATSSPIFSFAISLP
                     NNIVGIRLVDRSAVSAEGGAVAAGGTSRVDNVSINTVPEPSTFVFFSFAVVGFGGAAL
                     RRTQSRRRLTAAS"
     gene            complement(1782..2420)
                     /locus_tag="VFB80_12950"
     CDS             complement(1782..2420)
                     /locus_tag="VFB80_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZN34726.1"
                     /translation="MSSDAEAPKADCYSAGERGAMVYWQELRGWALGPLLTALSNCGL
                     TADHITAVSLTLGLAFCPLWLWPGSPPWAKPAALLALALHVLLDGLDGPLARHQGTAS
                     RRGSFTDTLADQIVVTATTLALMASNPPLVGVWAGGSYVFLYAMVVAFAMVRTALSVP
                     YTWLIRPRFWVYAWIAVELYLLAGWLELIIVAFSAVLAIKLATGFIAIRRLL"
     gene            complement(2483..3907)
                     /locus_tag="VFB80_12955"
     CDS             complement(2483..3907)
                     /locus_tag="VFB80_12955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN34727.1"
                     /translation="MNPRAFAILIVSPDRGTLRRLSKFLEVFGYDVRQATDGPEAIAA
                     AESSQPDFLILDGSSGKPADLQLCGQVRGVWPDRYSYCLLLVERPSVADITSALELGC
                     DDFLAAPIVFGELLARLRTGARVLEFERRLSEQAGFDPTTGLADKAGLAAALAKRASM
                     AKGSAGWLTLIDPDYFQRFTDRYGRRPAQDLLRQVADKVQARCGLEAFAASLGDDRLA
                     ILLPASGSETAVAWAEQVLASLAEETFTLGDQSLKLTASCGLTELGVGETLDVVLARA
                     QRSLQLAKSSGRNCVVTSNEVDRDAEQWNEIAADGKLFTTTQARDVMSPCPVLIHLDE
                     TFDQAHAMLQLAGLPSAPVVDGDGRLAGVVSLDQLAAARLRNPKARTTAANSSVRLVR
                     QVMSTEVTKFEEATPLSTLMEFFTGDGTPLAVVVRDKRPRGLIHCQGLASLNDRLTAD
                     HFVATKPRTGASEDLLVPDLAIAE"
     gene            4179..5333
                     /locus_tag="VFB80_12960"
     CDS             4179..5333
                     /locus_tag="VFB80_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN34728.1"
                     /translation="MRTTILPGTSSATAWLERDIPGKETERITLESFPFTLGRNESCD
                     YQILSSRVSREHAEIVREGGLYRLRDLKSTNGTFVNGKRIEEHRLTDGDLFVIADVEF
                     SFKRGGSDEVRKTVTQVMDSGRGELGEDRVDDAVADLILAVRRTHETLLHRAARNRFQ
                     PIFDLAENRCVGFEAIRRSQLPGDPPQAQQILDSTDCRLTERMNQIHRLIAAEHLARL
                     ADATMLFVSLQPAEVGADALPSSLARLAKLAGNKKIVAEIPDTAVVDIPYFRDFLARL
                     RDLKIGVAYDGFAGSPHQIKAQEQFPPDFLKLTPALARGVDKSTQRQQQIKALVEAAQ
                     QIGAQLIACGVHSENEARTCHELGCRLAQGDHFGRPQTIDWPIDGLSLGA"
     gene            5638..>6751
                     /locus_tag="VFB80_12965"
     CDS             5638..>6751
                     /locus_tag="VFB80_12965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34729.1"
                     /translation="MTPAANPSRTTNEPIPGYKLVKRIGAGGYGEVWTAEAPGGLVKA
                     IKFVYGLLDEDRAARELKALNRIKTVRHPFLLSLERIEVVEGQLLIVTELADCSLKDR
                     FDECKKAGLQGIEREELLSNMRDSADALDYMSEQHSLQHLDVKPENLLILGGRVKVAD
                     FGLVKDIHDHTASMMGGLTPVYAPPEVFEGRPSRKSDQYSLAIVFQEMLTGSLPFPGR
                     TAAQLAAQHLNAKPRLNGLSEADQAVLSKALAKKPADRYESCRAMLQALTDAGRAPSP
                     PKSDGPSPATTDVAGMPTSTRLPSEMLSNLDAATRAIQQLTSTGSIIQPSTLPPGVSR
                     PMRMTHVRKSMTAEEAAAFLGPTPELAALQTTPPLVD"
BASE COUNT         1247 a         2259 c         2187 g         1058 t
ORIGIN      
        1 tctttacgcc ggcggcccgc gtggagtcgc tcgtgcgggg ccgcgacgtg caaacggcct
       61 acgggcacta gggacgcgac aaacggcaga gcgcggggtt agaataagac acgttgtctg
      121 aatcgccgcc gcccaccgcg aggcctccca tgatccgccc gctcgcctgc ctaatggtcg
      181 ccgccggctc gctgctgctt ttggctgacg atgctccgcc cgcgaagaac aaggagcatg
      241 ccgcccagca ggagcggctg gagaaaatcc agcaactcgt gggcgcctgg cgcggtgtgg
      301 ctcagccgca gcgcggcagc acccgcgaca cctggatcga agaagccgac tgggcctgga
      361 gcttcggcaa ggagggcccg gcgctggtcg ccaaatcgcc gaaggccaaa ttcttcagcc
      421 agctctcgct tacgacgggc aaggacgccg gccagtacgt cctggcggcg acgccggcgg
      481 ccggtggcga ggcggtccgt tacgccggca cactggacga cgagcaacgg ctggtcctca
      541 cgctcgaaaa accgcagggc gacttgccgc agcggctcag cttccgcttc gtcgccggcg
      601 gcgatcggct gctggtgctg atggagaaaa aaagcccgac gagcgaccag ctcgtgcggc
      661 tggccgaagt cggctacacc cggcagggaa gcggttttgg caaaaacacc gctcagcggg
      721 agtgcgtcgt gacgggcggc ctgggcacga tcgaagtgat gcacaaaggc cagacctact
      781 acgtctgctg caccggctgc cgcgactatt tctacgagaa gcccgacgag attctcgccg
      841 aatacgtcgc ccgcaaggca gccgagaagg ccaagcggcc ttgaccggcc gttagagcag
      901 ccggcggagt gccacaatgc cggtggcgag attgagtgcc agaaccacgg agaacgccgc
      961 gacgatcggg tccggccaag acttcgcaat gctaattctt caaacattaa ctgtcaggaa
     1021 agcggcgtgg gcggcaaata ggagttgtta aggaaagtta gcgctgcccc cgcagatgag
     1081 ggaacgccga tgaagcgcct cttgcttgcc tctcttgtcc ttctcgtagc caaccaggcc
     1141 agcgcggccc tcatcgcacg ctggacgttc gaaacgagtc cacccgccga cttgaacaac
     1201 agcgccacga ttggtggaat tctggcggat gcagggatag gaactgcatc cggtgttcat
     1261 gcaagtgcag cgacagactg gtcaactcca gttggcaatg gctccggaaa ttcgctctcg
     1321 tcaaacgagt gggccgtcgg agactactac cagtttgcgt tcaacgcagc gggactaacg
     1381 aatctgcagc tgaacttcga ccagaccagc tccaacactg gcccgcgtga tttcaaggtc
     1441 caaacaactc tggacggcac acttttcacg gacttggtga actacgtggt gctggcaaac
     1501 gcggctccca atccattctg gaccagcgcc acaagctccc ccatcttctc atttgcgatc
     1561 tcccttccga acaacattgt gggtatacgg ctggtcgatc gctcagctgt ttccgcggag
     1621 ggcggtgcgg tcgctgctgg cggcacgtcg cgcgtcgata acgtcagcat caataccgtc
     1681 ccggagccat cgacctttgt ctttttcagc ttcgccgttg ttggattcgg cggcgcggcc
     1741 ctccgccgca ctcaatctcg cagacgattg actgccgcca gctaaagcag ccgacggatg
     1801 gcgatgaagc cggtcgccag cttgatcgcg aggacggccg agaaggcaac gataatcagc
     1861 tcgagccagc cggcgagcag gtacagctcg acggcaatcc aggcatagac ccagaaccgc
     1921 gggcggatga gccaggtgta cggcacgctc agggcggtgc ggaccatcgc aaacgcgaca
     1981 accatcgcat acaggaagac gtacgacccg ccagcccaca cgccgactag cggcgggttc
     2041 gaggccatca gcgcgagcgt ggtggcggtg accacgatct ggtcggccag cgtgtcggtg
     2101 aacgagccgc gacgcgacgc cgtgccctga tgcctggcga gcggcccgtc gaggccatcc
     2161 agcagcacgt gtagagccaa agccaagagc gccgccggct tggcccacgg cggactgccc
     2221 ggccagagcc agagcgggca aaacgccagg cccagcgtca acgaaaccgc cgtgatgtgg
     2281 tctgccgtca ggccgcagtt actaagcgcg gtaagcaggg gacccagcgc ccagccgcgg
     2341 agttcctgcc aatagaccat cgcgccgcgt tcgccggcgg aataacaatc ggccttcggg
     2401 gcttcggcgt cggagctcat gcgggcaccg cgaggaccgc gaccgctgtc gcgactctgg
     2461 aacgcggtgc attgtgatcg ctctattccg cgatcgccag atcgggaacc agcaagtcct
     2521 cgctggctcc ggtgcgaggc ttggtggcga cgaagtgatc ggccgtcagc cggtcgttga
     2581 gcgaggccag cccttggcaa tggatcaggc cccggggccg cttgtcgcgg acgacgaccg
     2641 ccagcggcgt gccgtcgccg gtgaagaact ccatcagcgt cgagagcggc gtggcctctt
     2701 cgaacttcgt cacttcggtg gacatcacct gccgcaccag ccgcacggag ctgttggccg
     2761 cggtcgtgcg cgccttggga ttgcggaggc gggccgcggc gagctgatcg agcgagacga
     2821 cgcccgccag gcggccgtcg ccatcgacga ccggggcgct gggcaggccg gcgagctgca
     2881 gcatcgcgtg ggcctggtcg aaggtttcgt cgagatgaat cagcacgggg cacggcgaca
     2941 tcacgtcgcg ggcctgcgtg gtggtgaaca gcttgccgtc ggcggcgatc tcgttccact
     3001 gctcggcgtc gcgatcgacc tcgttgctgg tcacgacgca gttgcggccg gagctcttgg
     3061 cgagctggag gctccgctgg gcgcgggcca ggacgacgtc cagggtctcg ccgacgccca
     3121 gctcggtcag gccgcagctg gcggtcagct tgagcgactg gtcgcccagc gtgaaggtct
     3181 cttcggccag cgaggccagc acctgctcgg cccaggccac cgccgtctcg ctgccgctgg
     3241 ccggcaacag gatcgcaagg cggtcgtcgc cgagcgatgc ggcaaaggct tccagcccac
     3301 agcgggcctg aaccttgtcg gccacctgcc gcagcaggtc ctgcgccggg cggcggccgt
     3361 agcggtcggt gaagcgctgg aaatagtcgg ggtcgatgag cgtcagccag ccggcgctgc
     3421 ccttggccat gctcgctcgc ttggccagcg cggcggccag gcccgctttg tcggccagac
     3481 cggtggtcgg atcgaagccg gcttgttcgg agagccgccg ctcgaactcc agcacccgtg
     3541 ctccggtccg cagccgcgcc agtaattcgc caaagacgat cggcgcggcc aggaagtcgt
     3601 cgcagcccag ttccagggca gacgtgatgt ccgcgacgga gggacgctcg acgagcagca
     3661 ggcagtagga gtaacggtcg ggccagacgc cgcggacctg cccgcagagc tgcaagtcgg
     3721 cgggcttgcc ggaactgccg tcgaggatca agaagtccgg ttgcgacgat tccgcggcgg
     3781 cgatggcttc cggcccatcc gtcgcctgcc ggacgtcgta gccaaacacc tccaggaact
     3841 tcgacaggcg gcggagtgtg ccgcggtcgg gcgaaacgat caaaatcgcg aacgcacggg
     3901 gattcatcgg cagatccttc ggtccggtgg agcgatgcca gtctgcgcgg tttcgcgcgc
     3961 ggaacttcgc aatccaagcc taggtcagga ttttccggcg cagcaaacgc gctaaacaaa
     4021 aacctgccgg gccggttgta gcggaaatac caaagaaccg aagaaccaaa ggcaacagta
     4081 ttcccttcga ttctttggtc cgttggttct tggcctttcg tcctgcatgc tacgtttcct
     4141 aaggcccgcg ccctcgtttc ggccgacttg gagcaatcat gcgcacgaca attctccccg
     4201 gcacttccag cgctaccgcc tggctggagc gcgacatccc cggcaaggaa accgaacgga
     4261 tcacgctgga aagcttcccc ttcaccctgg gccgcaacga atcgtgcgac taccagatcc
     4321 tctcctcgcg ggtctcgcgc gagcatgccg agatcgtccg cgagggcggc ttgtatcgac
     4381 tacgtgatct gaagagcaca aacggtacct tcgtcaatgg caagcggatc gaagagcacc
     4441 gcctgaccga cggcgatctg ttcgtgatcg ccgacgtcga gttcagcttt aagcgcggcg
     4501 gcagcgacga agtccgcaag accgtcacgc aggtgatgga ctccggccgc ggcgaattgg
     4561 gcgaggaccg cgtcgatgac gccgtcgccg atttgatcct cgccgtccgc cggacgcacg
     4621 agaccctgct gcaccgggcc gcccgcaacc gcttccagcc gatcttcgac ctggccgaga
     4681 accgctgcgt cggcttcgag gcgatccgcc gctcgcagct gcccggcgat ccgccgcagg
     4741 cccagcagat tctcgacagc accgattgcc gcctcaccga gcggatgaat cagatccatc
     4801 gcctgatcgc cgccgagcac ctggcccgcc tggccgacgc cacgatgctg tttgtcagcc
     4861 tgcagccggc ggaagtcggc gccgacgccc tgccgtcctc gctggcccgg ctagcgaagc
     4921 tggcgggcaa caagaagatc gtggccgaga ttcccgacac ggccgtcgtc gatattcctt
     4981 acttccgcga cttcctggcc cggctgcggg acctgaagat cggcgtggcc tacgacggct
     5041 tcgccggctc gccgcaccag atcaaggccc aggagcagtt cccgccggac ttcctcaagc
     5101 tcacgccagc cctggcccgc ggcgtcgata agagcacgca gcggcagcag caaatcaagg
     5161 ccctggtgga agccgcccag cagatcggcg cgcagctgat cgcctgcggc gtgcactccg
     5221 agaacgaagc ccgcacctgc cacgagctcg gctgccgcct cgctcaaggc gaccactttg
     5281 gccgccccca aaccatcgac tggccgatcg acggcctctc gctgggggcg taacaggctg
     5341 tccggtcggc ccggaagtgc cgacctacgt gcgctactct cccgcggcta tttcggtggg
     5401 cgaaggtctg tcttaacggt cgtgcggatt gtggcgcgaa gtggcgccgg gtgggaaaga
     5461 taattcgccc gcggtgaatc gtaagccaga gttcaggtgg acggtaggct gcgcgccgaa
     5521 taggcgagcg gccggcgtga gccggccgat cattcggtcc cgcccagaac gtcgctatcg
     5581 ggaggctcac gcctcccgct cgcctcactg acccctgact cctgacccct ccgcccagtg
     5641 acccctgctg caaacccctc ccgaactacc aacgagccga ttcccgggta taagctcgtg
     5701 aagcggatcg gcgcgggtgg ctatggcgaa gtctggacgg ccgaagcgcc gggcgggctg
     5761 gtcaaggcga tcaagttcgt ctatggcctg ctggacgagg accgcgccgc ccgcgaactc
     5821 aaggccctca accgcatcaa gaccgtgcgg cacccgttcc ttctctcgct ggagcggatc
     5881 gaagtcgtcg aagggcaact gctgatcgtc acggagctcg ccgattgcag cctcaaggac
     5941 cgttttgacg agtgcaagaa agccgggctg cagggcatcg agcgcgaaga gctgctctcc
     6001 aacatgcggg actcggccga cgcgctggac tacatgagcg agcagcactc gctccagcac
     6061 ctcgacgtca aacccgagaa cctgctgatc ttgggcggcc gcgtcaaggt cgccgacttc
     6121 ggcctggtca aggacatcca cgaccatacc gcctcgatga tgggcggcct cacgcccgtc
     6181 tatgctccgc ccgaagtgtt cgaaggccgc cccagccgca agagcgacca gtacagcctg
     6241 gcgatcgtgt ttcaggagat gctgacgggc tcgctgccgt ttccgggccg cacggcggcc
     6301 cagctggcgg cccagcacct caacgccaag ccgcgcttga acggcctgtc ggaagccgac
     6361 caggccgtgc tctccaaggc gctggcaaag aagcccgccg accggtatga gagctgccgt
     6421 gcaatgctgc aggcgctgac cgacgccggc cgcgccccca gcccgccaaa gagcgacggc
     6481 ccgtcccccg ccacgaccga cgttgcgggc atgccgactt cgacgcgcct gccgtcggag
     6541 atgctctcga atctcgacgc ggcgacgcgg gccatccagc agctcacctc gaccggcagt
     6601 atcattcagc cgtcaactct gccgccgggc gtgtcgcgtc cgatgcggat gacgcacgtc
     6661 cgcaagtcga tgacggccga ggaagcggcc gcgtttctgg gaccgacacc cgagctggca
     6721 gcgctacaaa ccacgccgcc gctggtcgat g
//
LOCUS       DASRJB010000496         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3519574, whole genome shotgun sequence.
ACCESSION   DASRJB010000496 DASRJB010000000
VERSION     DASRJB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519574"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFB80_12970"
     CDS             <1..539
                     /locus_tag="VFB80_12970"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HZN34730.1"
                     /translation="GGAAIATAAPAMPAAVMPAAPVPAPTLPMPAPRAPAMPAQPAPL
                     PTPSVGDPIAEAPGAIWYVRPPSGGQFGPARGDVMRKWIAEGRVSSDSLVWREGWSDW
                     QNAGKLFPNLQAANTPALAIAPAASGVTRAASSVSRYQSKKGGSSGGLSAGLLIFLAI
                     VCVALVAVLAYVLFTMNA"
     gene            599..1153
                     /locus_tag="VFB80_12975"
     CDS             599..1153
                     /locus_tag="VFB80_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34731.1"
                     /translation="MRMLSLLAATLLVAGPALAQEAAAPTNFSIGEGKLSFTAPAGWT
                     KKQPATRIVEAEFATPAAKGEETPGRLTAMGAGGTIESNIDRWIGQFVGEGGAAVKPK
                     QDKLTVSGCEIHVVDLAGTYKDTPGGPFAGGKTTLRENYRMLGAIVVTKDAGNYFLKL
                     YGPKAAIGEAEKGFQEMLKSLKVK"
     gene            1238..2674
                     /locus_tag="VFB80_12980"
     CDS             1238..2674
                     /locus_tag="VFB80_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34732.1"
                     /translation="MKVQQFLDHHGIARNPFAEEDAQTDPVFKEHCIESTYHPTWDKV
                     YGDPSEPATSIIFGEKGSGKTAMRLQLARHLEHYNERHPGKRLFVIHYDDFNPFLDRF
                     RDRLSKRNRKPERVLAQFKLWDHMDAVLSLAVTGLVDRVLEVKQPSQAVVCQISASDV
                     DSLDQNQARDLLILAACYDQSTAETFKGRWHRLRRRLRFQSWKSYWQLALGWGLTALL
                     VLLVLIIVAYGGQAWRNFWLARGVLGRMRVGIRETNPLRQVLMQFLPAELSGQPLPNK
                     DSTDDRYELLMKLQGLLLSLGHSGLIVLVDRLDEPHLVNGSAELMKALLWPMLDNKFL
                     KHPGLGLKLMLPIELTRFIEREDRDFYQRARLDKQNMIPSFEWTGEALYDVATARLKA
                     CAKPGAKPALADLFDSALTHQRMIEAFRMLRVPRRLFKFLYHLIASHCNAHTDQNPTW
                     QISTTLFESTLALSLRDQDAFDRGVGAG"
     gene            complement(2730..3323)
                     /locus_tag="VFB80_12985"
     CDS             complement(2730..3323)
                     /locus_tag="VFB80_12985"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN34733.1"
                     /translation="MTSESYFRIALVAVIVPTMAVAVWHRLRARSGERISHKDEGYLF
                     ALVLRLAGLWLWVVTFAYLLYPQSVLWASLPWPGWLRWIGLVTGALCIVLMYWTLSSL
                     GKNLTDTVVTRAQATLVTHGPYRWVRHPFYVTAALLMASLTLLTANWLIGVSSLAVMA
                     LLALRTPKEEQKLIERFGDDYRRYMATTGRYLPRLSR"
BASE COUNT          640 a         1154 c         1088 g          575 t
ORIGIN      
        1 ccggcggtgc ggccatcgcc accgctgcac cggcgatgcc cgcagccgtc atgcccgctg
       61 ccccggttcc cgcgccgacc ctgccgatgc cggccccgcg ggcgccggcg atgcccgcgc
      121 agcccgcgcc tttgcccacc ccgagcgtcg gcgatccaat cgccgaggcc cccggggcga
      181 tctggtacgt ccggccgccc agcggcgggc agttcggccc ggcccgcggc gacgtgatgc
      241 gcaagtggat cgccgagggc cgcgtcagca gcgactcgct ggtctggcgc gaaggctgga
      301 gcgattggca gaatgcaggg aaactgtttc ccaacttgca ggcggccaat acgcccgccc
      361 tggccatcgc gccggcggcc agcggcgtga cccgcgccgc gagctccgtc tcgcgctatc
      421 agtccaagaa aggcggcagc agcggtggct tgagcgcggg cttgctgatt ttcctggcga
      481 ttgtctgtgt ggcactggtt gcggtgctcg cctacgtgct ctttactatg aacgcctgat
      541 gctgcgtcgc gaccagaggc ggcagggcaa taccactttg ctcacccgag gaactaccat
      601 gcggatgctc tcgctgctgg ctgctacgtt gctggttgcc ggcccggcac tggcccagga
      661 ggccgcggca ccgaccaact tctccatcgg cgaaggcaag ctctcgttca ccgcgcctgc
      721 gggctggacg aagaagcagc ccgcgacgcg aatcgtcgag gccgagttcg ccactccggc
      781 cgccaaaggc gaggagactc ccggacgcct gaccgcgatg ggcgccggcg gcacgatcga
      841 aagcaacatc gaccgctgga tcggccagtt cgtgggcgaa ggcggcgcgg ccgtcaagcc
      901 caagcaggac aagctcaccg tcagcggctg cgagattcac gtcgtcgatc tggccggcac
      961 ctacaaggac acgccgggcg gcccctttgc cggcggcaag acgaccctcc gcgaaaacta
     1021 tcgcatgctc ggcgcgatcg tggtcaccaa agacgccggc aactacttcc tcaagctcta
     1081 cggccccaag gccgccatcg gcgaagcgga gaagggcttt caggagatgc tgaagagcct
     1141 caaggtgaag taggaaactt atcagttgtc agttgtcagt ggtcagttgg ttcaaggcca
     1201 atatcaactg actactgacg actgactact gacaaccatg aaagttcagc agttcctcga
     1261 ccaccacggc atcgcccgca atccgttcgc ggaggaggat gcgcagaccg acccggtctt
     1321 caaagagcac tgcatcgaga gcacctatca cccgacctgg gacaaggtct acggcgatcc
     1381 gtccgagccg gccacttcga tcatcttcgg cgaaaaggga tcgggcaaaa cggcgatgcg
     1441 gctgcagctt gcccggcacc tggagcacta caacgagcgg catccgggca agcggctgtt
     1501 cgtcatccac tacgacgact tcaatccgtt cctcgatcgc ttccgcgacc gcctctcgaa
     1561 acgcaaccgc aagcccgagc gggtgctggc ccagttcaag ctctgggacc acatggacgc
     1621 ggtcctctcg ctggcggtga cgggcctcgt cgatcgcgtg ctggaagtca agcagcccag
     1681 ccaggccgtc gtctgccaga tctcggccag cgacgtggac tcgctcgacc agaaccaggc
     1741 ccgcgacctg ctcatcctcg cggcctgcta cgaccaatcg acggccgaga ccttcaaggg
     1801 ccgctggcac cggctgcggc ggcggctgag gttccagtcc tggaaatcct actggcaact
     1861 cgcgctcggc tggggcctga ccgcgctctt ggtgctgctg gtgctgatca tcgtggccta
     1921 tggcgggcag gcctggagga atttctggct ggcccgcggc gtgctcggcc gaatgcgagt
     1981 cggcatccgc gaaaccaacc cgctgcggca ggtgctgatg cagttcctgc cggcagagct
     2041 ttccgggcag ccgctgccga ataaggacag cacggacgat cgctacgagc tgctcatgaa
     2101 attgcagggg ctgctcttgt cgctgggcca cagcggcctg atcgtgctgg tcgatcggct
     2161 ggacgagccg cacctggtca acggctcggc ggagctgatg aaagcgctgc tttggccgat
     2221 gctcgacaac aaatttctga aacatccggg gctgggcctc aagctgatgc tgccgattga
     2281 gctgacgcgg ttcatcgagc gggaagatcg cgacttttat cagcgagccc ggctcgacaa
     2341 gcagaacatg atcccgtcgt ttgagtggac gggcgaggcg ctctatgacg tagccaccgc
     2401 ccggctgaaa gcctgtgcca aacccggcgc gaagcccgcg ctcgccgacc tctttgattc
     2461 ggccctaacc caccagcgga tgatcgaagc tttccgcatg ctccgcgttc cccgccggct
     2521 cttcaagttc ctctatcacc tgatcgcctc gcactgcaac gctcacaccg accagaaccc
     2581 gacctggcag atctcgacca cgctcttcga atccacgctg gccctgtcgc tccgcgacca
     2641 agatgccttc gaccgcggcg tgggggcggg ataaggcaag gttgggctcg gtatttgggt
     2701 ttgtctgcgc aggtgaagtg acgggccaat cagcgtgaca acctgggcaa ataccggccc
     2761 gtggtggcca tgtaccgccg gtaatcgtcg ccaaatcgct cgatcagttt ctgttcctcc
     2821 ttgggggttc gcagggccag caaagccatg accgccaggc tgctgacgcc gatgagccag
     2881 ttggcggtca gcaacgtcag cgaagccatg agcagcgcgg ccgtgacata gaacggatgg
     2941 cgaacccagc ggtacgggcc gtgcgtgacc agcgtagcct gggcccgcgt gacgacggtg
     3001 tcggtgaggt tcttgcccag gctcgacagc gtccagtaca tcaggacgat gcacagagcg
     3061 ccggtgacga gcccgatcca gcgcagccac cccggccagg gcaggctcgc ccacaggacg
     3121 gattgcggat acagcaggta ggcaaaggtg acgacccaaa gccagaggcc cgccagccgg
     3181 agcacaagtg cgaacaaata gccttcgtcc ttgtgcgaga tgcgttcgcc cgatctggcc
     3241 cgcagccggt gccagaccgc gacagccatc gtcggcacaa tcaccgcgac tagcgcgatt
     3301 cggaagtagg attcggaggt catgcgccac attcctctag cccgatgccg ggcagccacc
     3361 gttgccgctg cctgggccat gcgcgcatag ccgacagaaa cttccgcgtg ccgttttcga
     3421 cataactcca gtgcctccag tctgttacgt catgcgc
//
LOCUS       DASRJB010000497         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_137953, whole genome shotgun sequence.
ACCESSION   DASRJB010000497 DASRJB010000000
VERSION     DASRJB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137953"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(479..>3342)
                     /locus_tag="VFB80_12990"
     CDS             complement(479..>3342)
                     /locus_tag="VFB80_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34734.1"
                     /translation="PAKIAGEPVAKLLDLLKEPEDRTRYRARIELSGRPTGEVLAATK
                     SWLASQNQSDPQYEHHALEALWLHQHHNVVDTSLLERMLASPDFHARAAAVRVLCYWR
                     DRASNSLDLLKKAAADQHPRVRLEAVRAASFFTAPEAVEVPIIAAEYPVDTYIDFVSR
                     ETMKTLDPYVQRALAANERINFSTDMGARYFLRNVSTEQLLKENRTRPVLLEMLLRPG
                     LQDPLRREAVASLAALDKKSELAVVIDTIKLLDAKETNVNVSVVFDLVRQLTGRSSRE
                     LAAARTELQRLATAARQPVFRQIGYVSLINVDGQIDPAWNLATKDAKALLDFVSAMPL
                     IADPTVRAALYEKVLPLLDSLPGPLAASAGKGTLGRYVRVELPGRGTLTLAEVEVTSG
                     GVNIARKGRASQKNTSSGGDASRGIDGNKSGVYGDGGQTHTEENTNRPYWEVDLGEEV
                     PIEEIAVYNRTEGTLGNRLDGFTLSVLDAKRGEVFKQDRIAAPAPSINVELSGSGPAA
                     SVRRAAMTAITQVRGKEGEAFTALAKFVIDGNDRLAAIRALQRIPRTYWAKDLAKPLL
                     EPIVADVKKTPVADRTSPAVLDSLEFADALVSLLPAGEAKLRRAELRELGVRVIRLGT
                     IFEKMAFDKEILVLQAGKPVEFLLENTDLMPHNFVIAQPGSLEEIGLLSEASAQDPKF
                     AVRQFVPLSPKVLAGSRLMQPRDSQRLSFTAPAAVGVYPYVCTYPGHWRRMHGALYVV
                     NDLDAYEANPEAYLAANAIPIKDELLKDRRPRTEWKLEDLADAVSHLESGRSFANAKQ
                     MFTAASCVACHKLDGVGNQLGPDLAQLDAKLQPLDLLKELLDPSAKINEKFQTQVFQL
                     ESGVTVTGLVIEETPAVIKLVENPLAKAAATEIKVADITARKKSATSIMPKGLLDKLT
                     REEILDLIAFLHARGKKDHAAFSAPAGGPGHGAHGGHGH"
BASE COUNT          516 a         1032 c         1169 g          625 t
ORIGIN      
        1 gccgaagttg gatagtaagc aacggtcgga gcgtagtagg cagtcgttgg agcgtagtag
       61 gcagtcatcg tcggagcgta gtaggcagtc gtcgtcggag cgtagtaggc agtcgttgtc
      121 ggagcgtagt aggcagtcgt agtcggcgcg tagtaggaag tcgttgtcgg cgcgtagtag
      181 gtagtcgttg tcggcgcgta gtacgtagtc gtcggagccg gcgcgtaata cgacgtagtt
      241 tgcagatatc caaattgagc atcagctacc tgtgtgaaaa caaaaatcga cgtccccgct
      301 acgataagtg ccagtgagtt cttcacgagt cttctcccga aaaagtcgtg tgcgattcga
      361 tcggaacaaa cgagtagaaa cgagaaatcc ttattcaaca agcagtaggg gggtgaaaag
      421 ggtcaggcga aattatttgg gtttcctcgg acgcccgcgg ggacggaggc accagccatt
      481 agtgcccgtg gccgccgtgg gcgccgtggc cggggccgcc ggcgggggcg gagaaggcgg
      541 cgtggtcctt cttgccgcgg gcgtgcagga aggcgatcag gtcgaggatc tcctcgcggg
      601 tcagcttgtc gagcagcccc ttgggcatga tcgacgtggc cgacttcttg cgggccgtga
      661 tgtcggcgac cttgatctcg gtcgcggccg ccttggccag cgggttctcg acgagcttga
      721 tcacggccgg cgtttcctcg atcaccaggc cggtgaccgt cacgcccgat tccagctgaa
      781 agacctgcgt ctggaacttc tcgttgatct tcgccgaagg atcgagcagc tccttcagca
      841 gatccagcgg ctgcagcttc gcgtcgagct gcgccaggtc ggggccgagc tggttgccga
      901 cgccgtcgag cttgtggcag gcgacgcagc tggcagccgt aaacatctgc ttggcattgg
      961 cgaacgagcg gccgctttcc aggtggctca ccgcgtccgc cagatcctcg agcttccact
     1021 cggtccgcgg gcggcggtcc ttcagcagct cgtccttgat ggggatcgcg ttggccgcca
     1081 gatacgcttc ggggttggcc tcgtaagcgt cgaggtcgtt cacgacgtac agcgcgccgt
     1141 gcatccgccg ccagtgaccg ggatacgtgc agacgtacgg gtaaacgccc accgccgcgg
     1201 gcgcggtaaa gcttagccgc tgcgaatccc gcggctgcat cagccggctg ccggcgagca
     1261 ctttgggcga cagcggcaca aactgccgca cggcgaactt tgggtcctgc gcgctcgctt
     1321 cggagagcag gccgatctct tccagcgagc cgggctgggc gatgacgaag ttgtggggca
     1381 tcaggtcggt gttctccagc aggaactcga ccggcttgcc cgcctggagg acgaggattt
     1441 ccttgtcgaa ggccatcttc tcgaagatcg tacccaggcg gatgacgcgg acgcccagct
     1501 cgcgcagctc ggcccgccgc agcttcgctt cgcccgcggg caagaggctg accagcgcgt
     1561 cggcgaactc cagcgaatcg aggaccgccg gcgatgttcg gtcggcgacc ggcgttttct
     1621 tcacgtcggc gacgatcggc tcgagcagcg gtttggcgag gtccttggcc cagtaggtcc
     1681 gcggaatccg ctggagggcg cggatcgcgg cgaggcggtc gttgccatcg atcacgaatt
     1741 tggccagcgc ggtgaaggcc tcgccttcct tgccgcggac ttgcgtgatg gcggtcatcg
     1801 ccgcgcggcg gacgctcgcg gcgggaccgc tgccggacaa ttcgacgttg atgctcgggg
     1861 cgggcgcggc gattcgatct tgcttgaaga cttcgccgcg cttggcgtcc aggacggaga
     1921 gcgtgaagcc gtccagtcga ttgccgagcg tcccctcggt gcggttgtag acggcgatct
     1981 cttcgatggg cacttcctcg cccagatcga cctcccagta gggccggttg gtgttttcct
     2041 ccgtgtgagt ctggccgccg tcgccgtaga cgccgctctt gttgccgtcg atgccgcggc
     2101 tggcgtcgcc gccgctggag gtgttcttct ggctggcgcg gcccttgcgg gcgatgttga
     2161 cgccgccgct ggtcacttcg acctcggcca aggtcagcgt gccgcggcca ggcaattcga
     2221 cgcgcacgta gcggccgagc gtgcccttgc cggcggatgc cgccagcgga ccgggcaaac
     2281 tgtccaggag cggcagtacc ttttcataca gggccgcccg caccgtcggg tccgcgatca
     2341 gcggcatggc cgagacgaaa tccaagagcg ccttggcgtc cttcgtcgcc aggttccagg
     2401 ccggatcgat ctggccatcg acgttgatca gcgacacgta accgatctgc cggaacaccg
     2461 gctgccgggc cgcggtcgcc aggcgctgga gctcggttct ggcggcggcc aattcgcggc
     2521 tgctgcgtcc ggtgagctgg cggacgaggt cgaacacgac gctcacgttg acgttggtct
     2581 ccttggcatc gagcagcttg atcgtgtcga tcacgacggc cagctcgctc ttcttgtcga
     2641 gcgcggccag gctcgccacg gcctcgcggc ggagcgggtc ctgcagtccc ggccgcagga
     2701 gcatctccag cagcaccggc cgcgtgcggt tttccttcag gagctgctcg gtgctcacgt
     2761 tgcgcaggaa atagcgagcg cccatgtcgg tcgagaagtt gattcgctca ttggccgcca
     2821 gggcccgctg gacgtagggg tcgagcgtct tcatcgtctc gcggctgacg aagtcgatgt
     2881 acgtatcgac cgggtattcg gccgcgatga tcggcacctc gaccgcctcc ggagcggtga
     2941 agaagctcgc ggcccgcacc gcctccagcc gcacccgcgg gtgctggtcg gcggcggcct
     3001 tcttcaggag gtcgagcgag ttgctggcgc ggtcgcgcca atagcagagc acgcggaccg
     3061 cagccgcacg ggcgtggaag tcgggcgagg cgagcatccg ctccaggagc gacgtgtcga
     3121 cgacgttgtg gtgctggtgc agccagaggg cctccagcgc gtgatgctcg tactgcggat
     3181 cgctctggtt ctgcgacgcc agccagctct tcgttgccgc gagcacttcg cccgtgggcc
     3241 ggccgctcag ctcgatgcgg gcgcggtacc gcgtgcggtc ctctggctct ttgagcaggt
     3301 cgagcagctt cgcaaccggc tcgcccgcga tcttggcggg cg
//
LOCUS       DASRJB010000498         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_987993, whole genome shotgun sequence.
ACCESSION   DASRJB010000498 DASRJB010000000
VERSION     DASRJB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987993"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFB80_12995"
     CDS             <1..900
                     /locus_tag="VFB80_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34735.1"
                     /translation="APVFDEALERVSPAVEAARGKVSSEIVPKLTEALSAAAAAPIVA
                     EATKRGKAAVAAAKGEVEPPKEKKGGWLKWLVIAAGLAGVAVFVVRRFLGGRDADWQA
                     ARPTTPYAPPRPAETARPTTAESAAGASFVDAAGADAGDDPQSGQSAHLLNLEADGSA
                     VPDQPAEGGDFEAAAETTAVVDESPETTMISDALGETTGPEAAESAAPGEAVADAAPA
                     EDQAATLLSEVAAPSGSGKRKYEGEGVYVGSEPPEGFTIKGNERSMKYHVPESAGYGR
                     TSAEVWFNSEEAAKEAGFVRAQR"
     gene            1044..1535
                     /locus_tag="VFB80_13000"
     CDS             1044..1535
                     /locus_tag="VFB80_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34736.1"
                     /translation="MTVRAEGAGLTGQWVDARPFRAQLHYLMASGSLTVDDVAAVAGI
                     SARVAERLLHGRDGRPLRRISPDTARRLIQISHQHVRALRRIMVPAGPARLQLRRLHR
                     AGWENLALAQQICVAAPELIELAAGAETCSLLLAVRLTAAARAEDGAHFRRPVGSPTE
                     HAA"
     gene            1573..>2186
                     /locus_tag="VFB80_13005"
     CDS             1573..>2186
                     /locus_tag="VFB80_13005"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZN34737.1"
                     /translation="MIEQALVIGAIALTIALTTALLARPVLQRLSEPAAGDGKPKYRD
                     LGSSRFLIACGVLAGVAATLSWLSLPRYAQPMWSVLAILGVLLAAIDARTSWLPLQLT
                     RVAWLAMAAASLLAAFLGGDPWVAVRAAAGAAIAAGLYLIVWLISRGGFGFGDVRFAP
                     LLGAAAAADSWQLLWLTLLLGTMVGGLVGLLRLALGRRDAFPYAP"
BASE COUNT          350 a          699 c          763 g          374 t
ORIGIN      
        1 gctccggtct ttgacgaagc gctcgaacgg gtcagtcccg ctgttgaggc cgcgcgcggc
       61 aaggtgtcca gcgagatcgt gcccaagctc accgaagcat tgagcgctgc cgcggcagct
      121 ccgatcgtgg ccgaggcaac gaagcgaggc aaagctgcgg tcgcggcggc gaagggtgag
      181 gtggagccgc ccaaggaaaa aaagggcggg tggctcaagt ggctggtcat agccgccggc
      241 ctcgccggag tcgcggtgtt cgtcgtccgg aggttcctgg gtggacggga cgccgattgg
      301 caggctgctc gaccaacgac gccgtacgcg cctcccaggc ccgcggaaac agctaggccc
      361 accaccgcgg agagcgcggc aggagcttct tttgtcgatg cggcgggggc cgacgcaggc
      421 gacgaccctc agagcggaca atcggcacac ctgctcaatc tcgaggcaga cgggtcagcg
      481 gttccagatc agccggctga gggcggcgac ttcgaggcag cggccgaaac caccgctgtc
      541 gtcgacgaat cgccggagac cacgatgatc agcgacgccc tcggcgaaac aacgggtccc
      601 gaggcggccg agtcggccgc gcctggcgag gcggtggccg atgcggctcc tgcagaggat
      661 caagcggcaa ccttgttgag cgaggtggcg gcgccttcgg gcagcggaaa gcgaaagtat
      721 gaaggcgagg gcgtctacgt cggcagcgag ccgcctgagg gcttcaccat caagggcaac
      781 gagcgatcga tgaagtacca cgttccagaa tcggccggct acggtcgcac ctccgccgag
      841 gtctggttca acagcgagga ggcggccaag gaagccggct tcgtccgcgc ccagcgctag
      901 gtctttgccg cacgttgcgc cgtaatttct tgcggtaatg gacgtctggt caagaggatc
      961 gtgcaagttt ccggcgcccg ttaacattcg gcctcttcgc gaagccttct acagatagga
     1021 ggcttcgacg aagggatttt ggcatgacgg tgcgtgccga aggcgctgga ctgaccggcc
     1081 aatgggtgga cgcgcgtccg ttccgcgcac aactgcatta cctgatggca agcggttcgc
     1141 tcaccgttga tgatgtagca gccgttgccg gcatttccgc tcgagtggcc gagcgcctgc
     1201 ttcatgggcg ggacggccgt ccgttgcgcc gaatcagccc cgacacggcg cgtcggctga
     1261 tccagatctc tcaccagcac gtgcgcgcac tgcgacggat catggtgccc gctggcccgg
     1321 ccaggctgca attgcggcgg ctccatcgcg cgggctggga aaatctcgcg ctggcccagc
     1381 agatctgcgt cgcggctcca gagctgatcg aattggcggc cggtgccgag acgtgctcct
     1441 tgttgctcgc ggtccgactg accgcggctg cgcgtgccga ggacggcgcc cacttccgca
     1501 ggccggtcgg gtcgccgacg gagcatgcgg catgagccag atcgcagggt cggacggcat
     1561 taggatcgcc gggtgatcga gcaggcgttg gtcattggcg ccattgctct cacgatcgca
     1621 ctgacgactg cactattggc gcgaccggta ctgcagcgac tttcagaacc agctgcaggc
     1681 gacggcaagc cgaagtaccg cgaccttggc agcagccgct tcctgatcgc ttgcggcgtc
     1741 cttgccggtg tggccgcgac gctcagctgg ctcagcctcc cccgctacgc ccaaccgatg
     1801 tggtccgtgc tggccatctt gggagtactg cttgctgcca tcgacgcacg caccagctgg
     1861 ttgccgctgc agctgactcg ggtggcctgg ctcgcgatgg ctgcggcctc gctgctggca
     1921 gcgtttctgg ggggcgaccc ctgggtcgcc gtcagggcgg ctgccggggc cgcaatcgct
     1981 gccgggctct atctgatcgt ctggctgatc tcccgcggtg gcttcggctt tggtgacgta
     2041 cggttcgcac ctctgctcgg cgcagccgca gccgccgact catggcaact gctgtggctg
     2101 accctgctgc tcggcaccat ggtgggcggt ctggtcggat tgctgcgact cgccctcggc
     2161 cgccgggatg cattcccgta cgcgcc
//
LOCUS       DASRJB010000499         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_421692, whole genome shotgun sequence.
ACCESSION   DASRJB010000499 DASRJB010000000
VERSION     DASRJB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421692"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2284)
                     /locus_tag="VFB80_13010"
     CDS             complement(<1..>2284)
                     /locus_tag="VFB80_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34738.1"
                     /translation="DRVLVEVLEGLPDEWNWSFVPPTPSERWTQRDLAFVELPRKYTS
                     HGVIDDRTSPLVLWAHTEMKLPTGKHRLLLRSRSAARLFVDGRLVVENAFQSGKTDGH
                     NAYKPVESAVSPRIRPLQPGDSENVAEAELSGGAHRLKLEIFVGGKKRRPEVGETSVS
                     IAPAGTDDFQVIGHVGDQTTSFPLTDAGWQAWEPATRAELIGINQRRRSEASAEYAKY
                     WDRRHSWARDLAGRASGLPYDSIDAYIDARLAAEKVAPAALIDDYGFVRRVYLDTLGV
                     TPTKDQVAAFIADPRADKRAKLIDELLAQPGWADNWVGYWQDVLAENPNIVNPTLNNT
                     GPFRWWLYEALLDNKPLDRFATELILMEGSQRYGGTAGFAVATENDAPLAAKAQNLGL
                     AFLACDMRCARCHDAPHHDFSQEDLFRLAAMLHRGTQTLPKTSTIPGDEKSHASLIVK
                     VTLKPGQKIAPQWPFDGEFQRELPAEWLANPADQREELAFRITSPQNPRFAKVFVNRL
                     WQRYLGRGLVEPIDDWETAQPTHPELLEWLARELLAHGYDARHIARLILNSRAYQRVP
                     TADADRARLVAAPLRRRMTAEQVLDSLLVAAGKELHVEELNLDVYGSRLETQSISLGL
                     PARAWQFTTMGNERDRPSLSLPGAQTAVTLLETFGWRASRQDPASYREKEPTVLQPAI
                     LANGVIARRIAQLSEDSAFVPLALAAETPADFVDSVYRQVLSRRPTADERALFVELLA
                     EDFSQRKTGAAPGPIPGWPAR"
BASE COUNT          345 a          777 c          790 g          372 t
ORIGIN      
        1 cgggcgggcc agccgggaat cggccccggg gcagcgccgg tctttcgctg gctgaagtcc
       61 tccgcgagca actcgacaaa cagtgcgcgt tcgtcggcag ttggcctccg cgacaggacc
      121 tggcgataga cggagtcgac aaaatcggcg ggcgtttccg cggccagggc cagcggcacg
      181 aaggcgctgt cctccgacag ctgcgcgatc cgccgggcga tgacgccgtt ggccaggatc
      241 gccggctgca agaccgtcgg ctccttctcg cggtagctgg ccggatcttg ccgcgaagcc
      301 cgccagccaa aagtttccag cagcgtgacc gcggtttgcg caccgggcag cgagagactc
      361 ggccggtcgc gctcattccc catcgtcgtg aactgccagg cccgcgccgg cagacccagc
      421 gagatcgact gcgtctccag ccggctgccg tagacatcga ggttcaactc ctcgacgtgc
      481 agctccttgc cggcggcgac cagcagtgaa tcgagcacct gctcggccgt cattcgccgc
      541 cgcagcggag cggcgacgag ccgcgcgcgg tccgcgtccg cggttgggac gcgctggtag
      601 gcccgcgaat tcaggatcag ccgggcgatg tgcctggcgt cgtagccgtg cgcgagaagc
      661 tcgcgggcga gccactccag cagctcggga tgcgtcggct gcgccgtctc ccaatcgtcg
      721 atcggctcga ccaggccgcg gcccaggtag cgctgccaga ggcgattgac gaaaaccttc
      781 gcgaaccgcg gattctgcgg cgacgtgatg cggaaggcca gctcctcgcg ctggtcggcc
      841 gggttagcca gccactcggc cggcagctcg cgctgaaatt caccatcgaa cggccactgc
      901 ggcgcgatct tctgcccggg cttgagcgtg accttgacga tcagcgaggc gtgcgacttc
      961 tcgtcgccgg ggatcgtgct ggtcttgggc agcgtctgcg tgccgcggtg cagcatggcc
     1021 gccaggcgga acaggtcctc ctgcgagaag tcgtggtgcg gcgcgtcgtg gcagcgggcg
     1081 caccgcatgt cgcaggccag gaacgccagg cccaggttct gggccttggc cgccagcggg
     1141 gcgtcgttct cggtcgcgac cgcaaagccg gccgtgccgc cgtaccgctg cgagccctcc
     1201 atcaggatca gctcagtcgc aaagcggtcg agcggcttgt tgtcgagcag ggcctcgtag
     1261 agccaccagc ggaacggccc ggtgttgttc agcgtcggat tgacgatgtt cgggttctcg
     1321 gccagcacgt cctgccagta gccgacccag ttgtcggccc agccgggctg cgccagcaat
     1381 tcgtcgatca atttcgcccg cttatccgcc cgcgggtcgg cgatgaacgc cgcgacctgg
     1441 tccttggtcg gcgtgacgcc gagagtgtcg agatagacgc ggcgcacgaa accgtagtcg
     1501 tcgatcaatg cggcgggcgc aaccttttca gcggcgagcc tggcgtcgat gtaggcgtcg
     1561 atcgagtcat agggcaggcc cgaggcccgc cccgccaggt cgcgggccca ggagtgccgg
     1621 cgatcccaat acttggcgta ttccgcggag gcctcgctgc ggcggcgctg attgatgccg
     1681 atcagctcgg cgcgcgtggc cggctcccag gcctgccagc cggcatcggt cagcggaaag
     1741 ctggtcgtct ggtcgccaac gtgtccaatg acctggaaat cgtccgtgcc ggccggagcg
     1801 atcgaaacgc tcgtctcgcc gacttccggc cgccgcttct tgccgccgac aaagatctcc
     1861 agcttcagcc ggtgcgcgcc gccggacaac tccgcctcgg cgacgttttc ggagtctccc
     1921 ggctgcagcg ggcgaatccg cggcgagacc gcgctctcga caggtttata ggcgttgtgc
     1981 ccgtcggtct tgcccgactg gaacgcgttc tcgacgacca gcctgccatc gacaaatagc
     2041 cgggcggcgc tccgcgagcg gaggagcagg cgatgcttgc cggtaggcag cttcatctcg
     2101 gtgtgcgccc agaggaccag cgggctcgtg cggtcgtcga tcacgccgtg gctggtgtac
     2161 ttgcgcggca gctcgacaaa ggccaggtcg cgctgcgtcc agcgctcgct gggcgtcggc
     2221 ggaacgaagc tccagttcca ttcgtcgggc aggccctcca gcacttccac cagcacccgg
     2281 tcgg
//
LOCUS       DASRJB010000500         6295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2677239, whole genome shotgun sequence.
ACCESSION   DASRJB010000500 DASRJB010000000
VERSION     DASRJB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677239"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFB80_13015"
     CDS             complement(<1..322)
                     /locus_tag="VFB80_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HZN34739.1"
                     /translation="MTRGFCLSVAFVLLIAATALAEDARPRLLVLTDIGGDPDDTQSL
                     VRLLVHSNEFEIEGLIASAAGVPGELKEAITRPDLIRQVVEAYGQVQPNLAKHAAAFP
                     SAENL"
     gene            383..682
                     /locus_tag="VFB80_13020"
     CDS             383..682
                     /locus_tag="VFB80_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34740.1"
                     /translation="MLIAWILIPLGLLAALYGFHRFMLYLENCDPFSSWRSASGGSGY
                     NPLLEIYQPQIRHIVQAQEQRLGDSEDDAGRPPLRRISRPPAPSCYWCHSPMKGR"
     gene            complement(649..2445)
                     /locus_tag="VFB80_13025"
     CDS             complement(649..2445)
                     /locus_tag="VFB80_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34741.1"
                     /translation="MAAQPANTWVKQTPLENSPVSPRLGYEGACVWDSKHQLLVRYGG
                     HNQGGGGEQGAEVWTYNPHTAVWKLMEPNTSPPGVCCNAQNVYCPPRERYVRFPFFSG
                     SHGWQWGRELYLNDSSVWTYDLSANQWRNMRPLPTPKLAPYRAAAWDSDRNVVVVFGG
                     ENSTEGTLIYDPLKNEWRWPKPPSEPEGRSGGSMAYDTSHRLHILFGSQFTDDPHTWA
                     YDVAKNQWRDMQPPSQPPTNKNDAVLTYDPLHQKVIGIIKVSMGEGDEATSELQTWAY
                     DAGANQWQRMSPAAEPQSSGNRARNLIFAPELNLAILENCTSRPREQQVWTYRYAEAP
                     VAAKPPAAAPSPLVEDVVVSVLNAKRIEISWTAAADPGVAGYHVERAAVEVYSDDQLQ
                     RLKSRTPPLAEPSVGAFRKIGPFESLTKEPTTALAFVDEGVDLDKPQVIEGDATYDRE
                     LHPEHVDAGGRPYRRAVFAYRVRSVNKRGESSGPSPAVFTIPSSPQQVFSREEGTTCQ
                     VKWAANPERGICGYRVYRMDGRFDKEPISRLTPDPLAATTYADPAAGKQSRRYYIVAV
                     DALGQEGLPSAPVWFQREWRQFYLPFIGEWHQ"
     gene            3135..4904
                     /gene="ilvB"
                     /locus_tag="VFB80_13030"
     CDS             3135..4904
                     /gene="ilvB"
                     /locus_tag="VFB80_13030"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZN34742.1"
                     /translation="MAAATTKPVDSQVLTGSDILVKSLVDHGVEVLFAYPGGASMPLH
                     QSLTKYADKIRTILPRHEQGGAFAAQGYARSSGKPGVCMATSGPGATNLVTAIADAKL
                     DSIPLVVITGQVKTDVIGTDAFQETPIVEVCRGITKHHYLVTDIKDIARVMKEAFFIA
                     TSGRPGPVLVDMPKNIQQESFQPDWDEPMNLPGYNGKLRMARPEQIKQVAAAIKLAKR
                     PVIYAGGGIIGGNASDELREFARKTGIPVTMTVMGLGAYPADGPLCLDMLGMHGSIYA
                     NWAVRDCDLLIALGVRFDDRVTGKLEAFAKHAKIIHVDIDPSEINKNKPAHIPICSDV
                     KYFLAELNKQVEPPADISTWVKQCLDWKRTQPFKFDVHFDGILQQHAIAELSRLSAGR
                     KTYITVGVGQHQMWSAQFYKFRQPRTFLSSSGLGTMGFGLPAAMGVQAAHPDALVVDI
                     DGDGSFQMNIQEMATCYCEKLPVKVLLLDNQHLGMVVQWEDRFHGSNRAHTYLGPIDH
                     AEATGRGGNHFVADRYPDFVAIAKGYRWGGATISSKADLVPALEEMINSKGPYLLNVQ
                     VPYVEHVLPMIPGGKTVDDMITE"
     gene            4940..5797
                     /locus_tag="VFB80_13035"
     CDS             4940..5797
                     /locus_tag="VFB80_13035"
                     /inference="COORDINATES: protein motif:HMM:NF040660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN34743.1"
                     /translation="MSQLQPFYEQVQAHYDLSNDFFELFLDRSLTYSCAYFERDDMSL
                     EEAQRAKIDLALGKCDLQPGQRLLDIGCGWGSAVARAAEKHGASCVGLTLSRRQFEYA
                     LRRLRHLEGLAQVRLQGWEEFDEPVDRIVSIGAFEHFRHERYPEFFARCYRLLPAGAP
                     MLLHSIVYPEDEVFGQPGMALTYDDVLFLKFIAKRIFPGGQLTRGSVVCRYAEAAGFR
                     VTRQHSLQPHYARTLEAWADNLAASRERAIELTSEETYDTYRHYLTGCAHYFRSGKID
                     VVQFTLRKP"
     gene            complement(5900..>6295)
                     /locus_tag="VFB80_13040"
     CDS             complement(5900..>6295)
                     /locus_tag="VFB80_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34744.1"
                     /translation="AVGCSQSGGPKLANVTGTVSLDGVPVEGAGLEFVADVGAVAYGR
                     TDASGQYYMSFGSSRTGAPLGKNLVRITAGDRVTVGDKKYESKEIFPKKYNVDSELSV
                     EVTSGSNHFDFDCKSAGEKLQQPISRGGN"
BASE COUNT         1130 a         2038 c         1972 g         1155 t
ORIGIN      
        1 acaggttctc cgcggacgga aacgccgcgg catgtttcgc cagattcggc tgcacttggc
       61 catacgcctc gacaacttga cggatcagat cgggccgcgt tatggcctcc ttcaattctc
      121 ccggcacgcc cgcagccgac gcaatcagcc cttcgatctc gaactcgttg ctgtgcacca
      181 gcagccgcac cagcgactgc gtgtcgtccg ggtcgccgcc gatgtcggtg agcacgagca
      241 accgcgggcg ggcatcttcg gccagcgccg tggcggcgat gagcagcaca aaggcaacgc
      301 tcaggcaaaa gccccgtgtc atgggtcgcg ccaagaagaa tggttctatg tcgaattaca
      361 tacagactgg agggttcata ccatgctgat tgcctggatt ctaatacccc tcggcctgct
      421 cgccgccctc tacggatttc accggttcat gctgtatctc gagaactgcg atccattttc
      481 atcgtggcgc agcgcttcgg gcggctctgg ttataacccg ctcctggaga tttatcagcc
      541 gcaaatccgc cacatcgtcc aggcccagga gcagcgcctg ggggacagcg aggacgacgc
      601 cggccggccg cccctgcggc ggattagtcg cccaccagcg ccaagctgct actggtgcca
      661 ctcgccgatg aagggcaggt agaactgccg ccactcgcgc tggaaccaga cgggcgcgga
      721 gggcaggcct tcctggccga gggcgtcgac ggcgacgatg tagtaccgcc gcgactgctt
      781 gccggccgcg ggatcggcat aggtcgtggc cgcgagcgga tcaggcgtga gccgcgagat
      841 cggctccttg tcgaaacggc cgtccatccg atagacgcgg tagccgcaga tgcctcgctc
      901 gggattggcg gcccatttga cctggcaggt cgtgccttcc tcgcgcgaga agacttgctg
      961 cggcgaggaa ggaatcgtga acactgccgg cgaggggccg ctgctctcgc cccgcttgtt
     1021 cacgctgcgc acgcggtagg cgaacaccgc gcggcgatac ggccggccgc cggcatccac
     1081 gtgctccgga tgaagttccc gatcgtaggt cgcgtcgcct tcgataacct gcggcttatc
     1141 cagatcgacg ccctcatcga cgaacgcgag cgccgttgtc ggctccttcg tgagcgattc
     1201 aaacggcccg atcttgcgga aggccccgac cgacggctcg gccagcggcg gcgtccgcga
     1261 cttcagccgc tggagctggt cgtcgctgta aacctccacc gcagcccgct cgacgtggta
     1321 gccggccacg cccggatcgg cggcggccgt ccacgaaatc tcgatgcgtt tggcgttcag
     1381 gaccgaaaca acgacgtcct cgaccagcgg cgacggtgcc gccgctggcg gcttcgcggc
     1441 cacaggagcc tcggcatagc ggtacgtcca aacctgctgc tcccgcggcc ggctggtgca
     1501 gttctcgaga attgccaggt tcagctccgg tgcgaaaatc aggttgcggg cccgatttcc
     1561 cgacgactgc ggctcagcag ccgggctcat tcgctgccac tggttcgcgc cggcgtcgta
     1621 ggcccaggtt tgcaattcgc tggtggcctc gtctccttcg cccatgctga ccttgatgat
     1681 gccgattacc ttctgatgca gcggatcgta ggtcagcacg gcgtcgttct tgttggtcgg
     1741 cggctggctc ggcggctgca tgtcgcgcca ttggttcttc gccacgtcat aagcccaggt
     1801 gtgcggatcg tcggtgaatt gcgacccgaa caggatgtgc agcctgtggg aggtgtcgta
     1861 agccatactt ccgccgctgc ggccctcggg ctcgctcggc ggcttgggcc accgccactc
     1921 gttcttcagc ggatcgtaga tcagcgtccc ctccgtgctg ttttcgccgc cgaagactac
     1981 gaccacgttc cggtcgctgt cccacgcagc cgcccggtag ggcgccagtt tcggcgtcgg
     2041 cagcggccgc atgttccgcc actggttggc cgacaggtcg taggtccaaa ccgacgaatc
     2101 gttgaggtac agctcgcggc cccattgcca gccgtggctg ccgctgaaaa acggaaagcg
     2161 gacgtatcgc tcgcgcggcg ggcaatagac gttctgggcg ttgcagcaga cgcccggcgg
     2221 cgaggtgttc ggctccatca gcttccagac cgcggtgtgc gggttgtagg tccagacttc
     2281 cgccccctgc tccccgccgc caccttgatt gtgcccgccg tagcggacca gcagctggtg
     2341 cttgctgtcc cacacgcacg cgccctcgta acccagccgc ggcgacacgg gcgagttttc
     2401 gagcggcgtc tgcttgaccc aagtgttggc aggctgagca gccaattgtc gagtctcttg
     2461 tcccgatgcg gggaccacgg acgggacgat tccgagcgcg agcaaacagg cgcgtaaaga
     2521 actttgcgtg cacatgctgg gtctccaaag cgggcggctc gtgcatcgtc aaacgtgaga
     2581 tgcgcggttg tagggcaaac cgggcggtga atgcaaccaa gcccaaggag aattggcagg
     2641 accctctttc gggggcctcc tcgtgcgccg accacaacct attgtggttt ggcggaggga
     2701 gaccacaatc tgttgtggtt gacttgacat atagtgtacg gatatttata gcctctaatt
     2761 cggagagagc cgcggataaa cgcggatgac acggatctga taggtgccga atccgtgtca
     2821 tccgtgttca tccgcggcca aagctgctta gtggcgggag gcgcgcataa tgcgcataat
     2881 cgccgggccg gcaaaactga cgcaagttgc tttgcagcaa ggaattgcgc cgcttttttg
     2941 ccgtcggacg ttttttgtga gtatgcgcgc atgatgggcc ggagcgatat tcctatgaac
     3001 cagtgcccgc ggcgcgacgt cggttgctgg tctggcgcac gtcctctatc ttgagtggtt
     3061 cccgctaggc gggggcgaat caggcggaaa tcgacgcaag ttcttgacca atcacgcttc
     3121 tctgaaagcc gaagatggcc gcagctacga ccaagcccgt cgattcgcag gtgctcacgg
     3181 ggtccgacat cctcgtcaaa tcgctggtcg atcacggcgt cgaagtgctg ttcgcctatc
     3241 cgggtggggc gagcatgccc ctgcaccagt cgctgaccaa atacgccgac aagattcgca
     3301 cgatcctgcc ccgccacgag cagggcgggg ccttcgcggc ccagggctac gcccgctcga
     3361 gcggcaagcc gggcgtctgc atggcgacca gcggaccggg ggcgacgaac ctggtcacgg
     3421 cgatcgccga cgccaagctc gacagcattc cgctcgtcgt catcaccggg caggtgaaaa
     3481 ccgatgtgat tggcaccgat gcctttcagg aaacgccgat cgtcgaagtc tgccgcggca
     3541 tcaccaagca ccactacctg gtgaccgaca tcaaggacat cgcccgcgtg atgaaggagg
     3601 cgttcttcat tgccaccagc ggccggccgg ggccggtgct ggtcgatatg cctaagaaca
     3661 tccagcaaga gtcgttccag cccgactggg acgagccgat gaacctgccg ggctacaacg
     3721 gcaagctgcg gatggcgcgg cccgagcaga tcaagcaagt cgcggcggcg atcaagctgg
     3781 ccaagcggcc ggtgatctat gccggcggcg gcatcatcgg cggcaatgcc agcgacgagc
     3841 tgcgcgaatt cgcccgcaag acgggcatcc ccgtaacgat gaccgtgatg ggcctgggcg
     3901 cttatcccgc cgacggcccg ctctgcctcg acatgctcgg catgcacggc agcatctatg
     3961 ccaactgggc cgtccgcgac tgcgacctct tgatcgccct gggcgtgcgg ttcgacgacc
     4021 gcgtgaccgg caagctcgag gcctttgcca aacacgccaa gatcatccac gtcgatatcg
     4081 acccctccga gatcaacaaa aacaagccgg cccacattcc gatctgcagc gacgtgaagt
     4141 acttcctcgc ggagctgaac aagcaggtcg agccgccggc cgacatctcc acctgggtca
     4201 agcagtgcct cgattggaag cggacgcagc cgttcaagtt cgacgtgcat tttgatggca
     4261 tcctgcagca gcacgcgatc gccgaattgt cgcggctctc agcgggccgc aagacgtaca
     4321 tcaccgtcgg agtcgggcag caccagatgt ggtcggccca gttctacaag ttccgccagc
     4381 cgcggacgtt cctctcgtcg agcggcctgg ggacgatggg atttggcctg ccggcggcga
     4441 tgggcgtgca ggcggctcat cccgacgcgc tggtggtcga tatcgacggc gacggcagtt
     4501 ttcagatgaa catccaggag atggcgacct gctattgcga gaagctgccc gtaaaggtgc
     4561 tgctcttaga caaccagcac ttgggcatgg tggtgcagtg ggaggaccgc ttccacggca
     4621 gcaaccgggc ccacacctat ctcggcccca tcgaccacgc cgaagccacc ggccgtggcg
     4681 gcaaccattt tgtcgccgac cgctaccccg acttcgtcgc catcgccaaa ggctaccgct
     4741 ggggcggcgc cacgatcagc agcaaagccg atctcgtgcc tgcgctggaa gaaatgatca
     4801 acagcaaagg cccctacctg ctcaatgtgc aggttcccta cgtcgagcac gttctgccca
     4861 tgatccccgg cgggaagacc gtggatgaca tgatcaccga gtgaccgaag gattcgcgct
     4921 cggctcaagc agcatggcta tgagccagtt acagcccttt tacgaacaag tccaggcgca
     4981 ctacgacctg tcgaacgact tctttgagct gtttctcgat cggtcgctga cctatagctg
     5041 cgcgtacttc gagcgcgacg acatgtcgct cgaagaggcc cagcgggcga agatcgacct
     5101 ggcgctgggc aagtgcgact tgcagcccgg ccagcggctg ctggacatcg gctgcggctg
     5161 gggttcagcc gtcgcgcggg ccgccgaaaa gcacggcgca tcgtgcgtgg gcctgacgct
     5221 cagccgccgg caattcgagt acgctctccg ccggctgcgt cacctggagg gcctggccca
     5281 agtccggctg caaggctggg aagagttcga cgagccggtg gaccggatcg tgtcgatcgg
     5341 cgcgttcgag cattttcgcc acgagcgcta tccggagttc tttgccaggt gttatcgcct
     5401 gctgccggcc ggcgcgccca tgctcttgca ttcgatcgtc tatcccgagg acgaagtgtt
     5461 tggccagccg ggcatggccc taacgtacga cgacgtgctt ttcctgaagt tcatcgccaa
     5521 gaggatcttc cccggcggcc aattgacgag aggctcagtc gtttgccgct atgccgaggc
     5581 agccggcttt cgcgtcacgc ggcaacactc gctgcagccg cactacgccc gaacgctcga
     5641 ggcctgggcc gacaacctgg cggcttcccg cgagcgggcc atcgagctga cctccgagga
     5701 aacctacgac acctaccgcc actacctcac cggctgcgcc cactatttcc gcagcgggaa
     5761 gatcgacgtg gtgcaattca cgctgcggaa gccgtgagcg gggcgcgtgg caccacccga
     5821 tttacatcct ggagaccgcc ggagcggtaa ccactttgtc gaagagcgtt atccgactcg
     5881 tcgccaccgc ggccagcggc tagtttccgc cgcggctgat gggctgctgc agcttttcgc
     5941 cggcggactt gcagtcgaaa tcgaagtggt tgctcccgct cgtcacttcg acagacaatt
     6001 ccgaatcgac gttgtatttc ttggggaaga tctccttgga ttcatatttc ttgtcgccga
     6061 cggtgactct atctccggcc gtgatccgga cgagattctt tcctaacggc gcaccggttc
     6121 ggctggagcc aaagctcata tagtattggc ccgacgcgtc ggtccgcccg taggcgacgg
     6181 cgcccacgtc ggcgacgaat tccaggccgg cgccctccac cggcacgccg tccaggctga
     6241 cggtgccggt cacattcgcc aacttggggc cgccggactg cgaacagccc acggc
//
LOCUS       DASRJB010000501        13471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_701660, whole genome shotgun sequence.
ACCESSION   DASRJB010000501 DASRJB010000000
VERSION     DASRJB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13471
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701660"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..395)
                     /locus_tag="VFB80_13045"
     CDS             complement(45..395)
                     /locus_tag="VFB80_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34745.1"
                     /translation="MRAILAAAVMLLAAGQACGAEARPNILLIYADDQSPKTLSCYPQ
                     AYPWAKTPQIDDLARRGVRFSHCYLGSWCMPSRANMLTGRYPHAIESMRMEGEYPGST
                     YDPAKCPFWPRVFR"
     gene            578..3760
                     /locus_tag="VFB80_13050"
     CDS             578..3760
                     /locus_tag="VFB80_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN34746.1"
                     /translation="MAALRPSRLTPLVLVLLALLPLYAPAAPPATAPVQGLAQNTPAV
                     FALTNLRIVPEPGKSIETGTIILRDGVITAAGAEVQPPADARVVDLAGKTAYAGLVDA
                     YGEQAASVDPAKQGAPSWNAQVAPQLDLAEHYAADEALNNKLRSQGIAARLVAPSQRI
                     IKGQSVVVSTAPGDNAHVILKNGVAQHFRLTVSFGASRGEYPGSPMGAVALARQTLLD
                     ADWYGKAWAAWRGDNKLPRPERNDALAALADTLNGNQPAIFNAANEQFFLRADRFARE
                     FGLAAIMHGSGREYRRLDEIRATGRAVIVPVNFPQPPSVATVEDALDASLADLMHWDI
                     APENPARLDAAGVTFALTSHGLRDEATFLAAVRRAVERGLAPQTALAAVTTTPASLLG
                     LGDRLGKIAPGMLGNLVIADGDLFDKQTKLLETWVDGRRFEHDKPPAVDVRGTWQLEA
                     AGPDDRVLRLKLKLAGPARQPTGAVSKVVSEGEKADEIRLAQVSLTELQLGFRFEGKL
                     FGKEGPARVTATIRSDDDSKLTLLGAVIWPDGTSERLTGERTARLTPEEEKAAAAQKS
                     KGKGKAKGEDGVTEGRSEGETETKEAAASFAVNYPFGDFGRPAQPAASNAAPQQPKLI
                     AFKNAALWTCGPAGTIEAGTLVVREGKIVAAGKEAEIPEGAVVIDAAGKTITPGIIDC
                     HSHMASDGGINEGTQAITAEVRIGDFIDETDITIYRQLAGGVTAANILHGSANPIGGQ
                     NQVIKLRWGLGGEQLKFAAAPAGIKFALGENVKRSNDGEVSTRYPQSRMGVEQIIRDA
                     FGRARDYAAAWERWNAAHAGLPPRRDLELEAIADILAKRRWIHCHSYRQDEILALIRT
                     CDDYGITIGSLQHILEGYKVADVMARHGATGSSFSDWWAYKIEVWDAIPYNGALMHRA
                     GVVVSFNSDDAELGRHLNHEAAKAVKYGGVPPIEALKFVTLNPAKQLRIDPWVGSLEA
                     GKDADFVVWSGDPLSPRSLCQETWIDGRKYFDRAADQSLRSEQAKMRAALIRKVIDSG
                     QATQAAGQREPEEWELWPRHDEYCEHGHDH"
     gene            3813..5114
                     /locus_tag="VFB80_13055"
     CDS             3813..5114
                     /locus_tag="VFB80_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN34747.1"
                     /translation="MRIIFCVACFCLTATAFANPEVPGAPQKKPVAITNVTIHPVSGP
                     VIAGGTLVFDKGKIAALGKDVTVPADAEVIDAAGKHVYPGLFDALTDLGLVEINSIRA
                     TIDGLEVGQLNPNVRALVAVNPDSEIIPVTRANGVLLALAAPYGGLMSGRSAVIQLDG
                     WTWEDLALAGDAGMHIQWPQYGAGGRGRRGGTPPSEAGPPRSEQAIEQLRQALADARA
                     YGAARQADPNFPRDARWESLQDVVGGKLPVIIHADDLRQLQAAVAFAQREKLKLIVAG
                     GYDARECLPLLKKHDVPIIVGGTYRLPRRRGDAYDSAYALPAALHAAGIRYCIGTQGR
                     FGASNVRNLPYHAAAAIGFGLPADEALKAITLYPAQILGASARVGSLEVGKDATLIIA
                     DGDVLDTPTQVTAAWIAGRKVELNDRHKRLYRKYEQKYQQP"
     gene            5448..5747
                     /locus_tag="VFB80_13060"
     CDS             5448..5747
                     /locus_tag="VFB80_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34748.1"
                     /translation="MEFDEVANKVLQSGGELVELKADLRQLQARVDRNTLVIQVLKEM
                     LLAQGGFREEEFADRLQRAAAQRTADKACRKCGKPLNPKHNRCMYCGEGRPPELL"
     gene            complement(5822..7081)
                     /locus_tag="VFB80_13065"
     CDS             complement(5822..7081)
                     /locus_tag="VFB80_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34749.1"
                     /translation="MSDSDRLLERIEEGVVAHGGDLGGWTRRSDDSLQLFAGRVTLRA
                     ELKDAGPSATDGMVHAHVLTTLHEHEDEALDACLYGMGDDREGALGQAAAIWITGVAG
                     PIKSFLDNKPICMTCQAGVAGGDLAGGYSPNDYGLTGLRAFVGPSLSRGIEDDRVMAA
                     IDDRKPWFRYAAESAAPRRVHLAKVTIVSKGKEGWDRELEIDGHDVSHLDPNWPAGVR
                     GPDFGYLTRFAVFEFPRNSTEIQRRAELERTIRHFALHLPKYDSVDRLMAEMVGQGFD
                     PDLVQETESISTIAFGRMLFEHLGVQYSSTIIRARRDGRIQTDVPLMGIPAYSRARAL
                     AVSLRDAMSEDAFQSLCLYNAESHALVQALNAAGDKPDLSSMKMFPCVVPERGVSGQT
                     MDAALATLNRLVERDRQAAKKPWWKFW"
     gene            complement(7095..9224)
                     /locus_tag="VFB80_13070"
     CDS             complement(7095..9224)
                     /locus_tag="VFB80_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN34750.1"
                     /translation="MKNLLPAVLLVLIGGWAAAQDAATAPAKVDTSRGDRMLAAYFAA
                     ETAQIQKNCLAEIKTLDDWNAKKEECRRQLREMLGLDPLPERTDLKPVITGTLEQDEF
                     RVEKLHFQSRPGLYVTGNFYLPKKVDKSLPTILYVCGHSVNAKDGVPLGAKVGYQHHG
                     AWFARNGYACLTIDTLQLGEIEGIHHGTHRYGMWWWLNRGYTPAGVEAWNCVRALDYL
                     ETRPEVDKSRFGVTGRSGGGAYSWWIAALDDRIKCAVPTAGITDLQNHVVDGCVEGHC
                     DCMYLLNTYRWDYPQVAALVAPRPLLISNSDSDGIFPLDGVYRTFEQVRRIYRLHEPV
                     EKGVTAKVALNIAAGPHKDTQELQTSAFRWFNHYLKGDDSLIDTRAPKFFAPEQLKVF
                     GKLPDDQINTKIHETFVAKAPPPEVPADKAAWEKMREGWLKALEEKSFRGSRIHFTTS
                     SADPEPFTPRSADFVAAADRDGLRISVYDCGWGIQPRLIVVHRLGLEKADLKVLTVLD
                     EARWSELLATFRDVDLEMFRNHPGPTNAEGWKSTQQMLQKNAWVMMYVAPRGIGATSW
                     DQSARKQVQNQRRFYLLGQTLDAERVWDVHLACTCSQSIPAWKEAPLWLQSHGPMAGV
                     TLYTSLFESDVKRLDLYDLPKSHRDGPYFLNVERYLDMPQAVAMAAERSKVVLYQDDD
                     SGWEYPQSVAKQLGWDAKQIQIRRKPK"
     gene            9467..10594
                     /locus_tag="VFB80_13075"
     CDS             9467..10594
                     /locus_tag="VFB80_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34751.1"
                     /translation="MRRGRTGFTLVELLVVVAIIGILMALLLPAVQAARESARRTQCY
                     NNLKQLALACHNFEQNHKKLPPYFGVFPERGVGAVDGGWFLHILPYIEQQQITDAVIG
                     AGGGMGRTQVLVTPASPDYTPSQTIYPPGGHWETVDGTGGDGSSHQGHTWPHTTPPNR
                     VWVGPPPITIPGTGTPPVFEFVNHGIDVIDSAGFSVLKCYSDPSRAQPNQRVSFRFGR
                     WSLTNYLGNYHLFAVNGVRTKQRRLAEVPDGLSNTVLAAEGMRLCDGTYRLALWGHYQ
                     FQHSHNFGVDWNGVPNTFMFQAVPHHTKCNNWRVQGLHFGRLSVAFADGSVRSLGKGM
                     TRREESDPDSPEWGIDPVIGSESGTWDRLLMAEDGEALGDY"
     gene            complement(10591..11724)
                     /locus_tag="VFB80_13080"
     CDS             complement(10591..11724)
                     /locus_tag="VFB80_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN34752.1"
                     /translation="MQERLAILGASTRAAAFSAVKAGIAPYAIDLFADRDLAAMCPAV
                     KIDRYPADFPAALASATPSPWIYTGGLENHPRLVDRLAAERPLLGNGGRVLRKVRDPA
                     RLAAVSTDTGCLFPEWRAAPPVQSGNVWLLKPRRSSGGQSIRFAAPEDYGRTLRATYW
                     QEYVEGQSASSVFVAARGQAVLLGTSRQLTGRDFGLDRPFLYAGSIAPLELPPNQTQR
                     LTRLGNLLAGRFELQGLFNVDFLANRDGLWVLEVNPRYSASVEILERLGGLSFVRLHV
                     DACRHQSLPDEPRQQVATFAGKAVVYARRASVVAGEFEALVSQWNRAGEPPGIADLPR
                     IGQHLAAGEPVATVLAGGESAEEVEAALRAQASAAQETLCPAV"
     gene            11817..12866
                     /locus_tag="VFB80_13085"
     CDS             11817..12866
                     /locus_tag="VFB80_13085"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HZN34753.1"
                     /translation="MRRRPRPLTDLADGDRVVLFPSLGQLSTDQRHWIVQIHGDVSAA
                     GHMTLGKRLLLKLLQRTMRATNDDFASPLFQQRIARFVAQDRPGKRIAVRIGEAVVPL
                     PKTTRRNGHFQSLVKVPCEEVAESGSPAAQELPLAVHSLTCEAPLPGRAFLLAPRGLS
                     VISDIDDTLKHSFVACKRTLLANTFLRPFETIPGMAGLFRDWAAAGAAIHYVSSSPWQ
                     LYQHLAEHLQAEGFPDGSFHLRAFRLRDHLLRRLLMLRRSGKIGVIRNILRMYPQRRF
                     LLIGDSGEHDPEIYGALARRFPQQVAGVLIRQLDDPRSSPQRFTKAFRHVDPLLVRLY
                     RDSGELSAVRLPEMA"
     gene            complement(13253..>13471)
                     /locus_tag="VFB80_13090"
     CDS             complement(13253..>13471)
                     /locus_tag="VFB80_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN34754.1"
                     /translation="MLTLFFNQERPADWEGASRSDTARYARYFWGLIDRGVYMPCSQY
                     EALFVSAAHTEAHIDATIQAAGEVFAEL"
BASE COUNT         2379 a         4428 c         4374 g         2290 t
ORIGIN      
        1 gtgccggtgt gccacttgcc gatctgcgcc gtttgatacc cgtgctaacg gaaaacgcgc
       61 ggccagaagg ggcatttggc gggatcatac gtgctgccgg gatactcccc ctccattcgc
      121 atcgactcga tcgcgtgcgg ataccgcccg gtcagcatgt tggcccgcga cggcatgcac
      181 cagctgccca ggtaacagtg cgagaaccgc acgccgcggc gggcgaggtc gtcgatctgc
      241 ggcgtcttgg cccaagggta tgcctgcggg taacaactca gcgtcttggg ggactgatcg
      301 tcggcgtaga tcagcaaaat gttggggcga gcctccgcgc cgcatgcctg ccccgccgcc
      361 agcagcatca ctgctgccgc caagatcgca cgcattcgca tgtccacgcc ctttcctctg
      421 tgtgctctgt gtctctgtga gagaactcgc ggcctcaatc ctaccgccct acggcctgta
      481 ccgcaaaaac gtgcaactcg ccagccggca tgttagagtt gctctcgaac attttgcgag
      541 ttttcaccca gttttgcttc cctgaggtcg gctgcctatg gctgcgctcc gcccatcccg
      601 gctcacgccg ctggtcctcg tcctgctcgc gttgctgccc ctctatgccc cggctgcacc
      661 acccgcgacc gcgccggttc agggcttggc gcagaacaca ccggccgtgt tcgcgctaac
      721 caacttgcgg atcgttccgg agccgggcaa gtcgatcgag acgggtacga tcatcctccg
      781 cgacggcgtc atcacggctg caggagccga ggtgcagccg ccagccgatg cgcgggtcgt
      841 cgatctggcg ggcaagaccg cgtacgccgg gctggttgat gcctacggcg agcaggccgc
      901 cagcgtcgat cctgcgaagc agggcgcgcc gagttggaac gcgcaagtcg cgccgcagct
      961 cgacctggcg gaacactatg cggctgacga agcgctgaac aacaagctcc gcagccaggg
     1021 catcgccgcg cggctcgtcg cgccgtcgca gcgaatcatc aaaggacaga gcgtcgtcgt
     1081 ttccaccgcg ccgggcgaca acgcccacgt gatcttgaaa aacggagtcg cccagcactt
     1141 ccgcctgacg gtcagcttcg gcgctagtcg aggcgagtat ccgggttcgc cgatgggcgc
     1201 agtggccctg gcccggcaga cgctgctcga cgccgactgg tacggcaaag cctgggcagc
     1261 gtggcgcggc gataacaagc tgccgcggcc ggagcgcaac gacgccctgg cggcattggc
     1321 agacacgctc aacggcaatc agccggcgat cttcaacgcc gccaacgaac agttcttcct
     1381 gcgggccgac cggtttgccc gcgaattcgg actggcggcg atcatgcacg gctcgggccg
     1441 cgagtatcgc cggctggacg agattcgcgc gaccggccgg gctgttatcg tgccggtcaa
     1501 tttcccccag ccgccgagcg tggccaccgt ggaggacgcg ctcgacgcat cgctagccga
     1561 cctgatgcac tgggacatcg cgccggaaaa tccggcgcgg ctcgacgcgg ccggcgtgac
     1621 gttcgccctc acgagccacg gcctgcgcga cgaggcgacg tttctggccg cggtgcgccg
     1681 ggcggtcgag cgcggcctgg cgcctcagac cgcgctcgcg gccgtcacga caactcccgc
     1741 gtcgctcctc ggcctcggcg accggctggg caagatcgcg ccggggatgc tgggcaacct
     1801 ggtcatcgcg gatggcgatc tgttcgacaa gcagacgaag ctgctcgaga cctgggtcga
     1861 tggccgccgg ttcgagcacg acaagccgcc ggcggtggat gtgcgcggga cgtggcagct
     1921 ggaagcagcc ggtcccgacg accgggtgct gcgactgaag ctgaagctgg ccggcccggc
     1981 gcggcaaccg acgggagccg tctcgaaggt agtcagcgag ggcgaaaagg ccgatgaaat
     2041 ccggctggca caggtttcgc tcactgaatt gcagctcggc tttcgtttcg aaggaaagtt
     2101 gttcggcaag gaagggccgg cacgtgtgac ggccacgatc cgctcggatg acgacagcaa
     2161 gctgacgctg ctcggcgcgg tcatctggcc cgacggcacg agcgagcgcc tgaccggcga
     2221 gcggacggcg aggctcacgc cggaggaaga gaaggccgcc gctgcgcaga aatcgaaagg
     2281 gaagggcaaa gccaagggag aggacggagt gacggaggga agaagtgaag gagagacgga
     2341 gacgaaggag gcggccgcca gcttcgcggt gaattatccc ttcggagatt ttggccggcc
     2401 cgcgcagccc gccgccagca acgccgcccc gcagcagccg aagttgatcg ccttcaaaaa
     2461 tgcggcgctc tggacctgcg ggccggcggg cacgatcgag gccggcacgc tggtggtgcg
     2521 agagggaaag atcgttgcgg ccggcaagga agcggagatc ccggaaggcg cggtcgtgat
     2581 tgacgcggcg ggcaagacga tcacgcccgg catcatcgat tgccactcgc acatggccag
     2641 cgacggcgga atcaacgaag gcacgcaggc catcacggcc gaagtgcgga tcggcgactt
     2701 tatcgacgag accgacatca cgatctatcg ccagctcgcc ggcggcgtca ctgcggcaaa
     2761 tatcctgcac ggctctgcca acccgatcgg cggccagaac caggtgatca agctccgttg
     2821 gggcctgggc ggcgagcaac tcaagtttgc cgccgcaccg gcaggcatca agttcgccct
     2881 gggcgaaaac gtcaagcgga gcaacgatgg cgaggtttcg acgcgctatc cgcaaagccg
     2941 gatgggcgtt gagcagatca ttcgcgacgc attcggccgg gcccgcgact atgccgcggc
     3001 ctgggagcgg tggaacgcgg cccacgcagg gctgccgccg cggcgcgacc tggagctgga
     3061 ggcgatcgcg gacatcctgg ccaagcgccg ctggattcac tgccacagct accggcagga
     3121 cgaaatcctt gccctgatcc gcacctgcga cgactacggg attacgatcg gctcgctgca
     3181 gcacatcctg gagggctaca aggtcgccga cgtgatggcc cggcacggag cgacgggttc
     3241 gtcgttcagc gactggtggg cgtacaagat cgaagtctgg gacgcgatcc cctacaacgg
     3301 cgcgctgatg caccgcgcgg gcgtggtcgt gtcgttcaac tccgacgacg ccgagctagg
     3361 ccggcacctg aaccatgagg cggccaaggc cgtcaaatat ggcggagtgc cgccgatcga
     3421 ggcactaaag ttcgtcacgc tcaatccggc caaacagctc cgcatcgatc cgtgggtcgg
     3481 ctcgctggag gccggcaaag acgccgattt cgtcgtctgg tcaggggatc cgctttcgcc
     3541 tcggtcgctc tgccaggaga cgtggatcga cggccgcaag tatttcgacc gcgccgccga
     3601 ccagtcgctt cgcagcgaac aggccaagat gcgggccgcg ctgattcgca aggtcatcga
     3661 ctccggtcag gccacgcaag cggcgggcca gcgcgagccg gaggaatggg aactctggcc
     3721 gcggcacgac gagtactgcg agcacgggca cgaccactaa caaagccgcg accattggtc
     3781 gcgctctagt cagactaccg agaatctcat ccatgcgaat cattttttgc gtcgcttgtt
     3841 tttgcctgac cgcgactgcc ttcgccaacc ccgaagtgcc cggcgcgccg cagaagaagc
     3901 ccgtggcgat caccaatgtc acgattcatc ccgtcagcgg gccggttatc gccggcggca
     3961 cgctggtgtt cgacaaaggc aagattgccg cgctgggtaa ggacgtcacc gttccggcgg
     4021 acgccgaggt catcgacgcc gcaggcaagc acgtctatcc cggcctgttc gacgcgctca
     4081 ccgacctggg gctggtggag attaactcga ttcgggccac gatcgacggc ctggaggtcg
     4141 gccagctcaa ccccaacgtc cgcgcgctcg tcgcggtaaa tcccgacagc gagatcattc
     4201 ccgtcacccg cgccaacggc gtgctgctgg ccctcgccgc gccttatggc gggctgatgt
     4261 ccggccgctc ggccgtgatt cagctcgacg gttggacctg ggaggacctc gcgctggcgg
     4321 gtgatgccgg aatgcatatc cagtggccgc aatacggcgc gggcggccgc ggccgccgcg
     4381 gcggcactcc gccatcggaa gccggcccgc cgcgcagtga gcaggccatc gagcagctgc
     4441 ggcaggcatt ggccgatgcg cgagcctacg gcgcggcccg ccaggccgat ccgaacttcc
     4501 cgcgcgacgc ccgctgggaa tctctccagg atgtcgtcgg cggtaagctg ccggtgatca
     4561 ttcacgccga cgacctgcgg caattgcagg cggccgtggc cttcgcccag cgcgagaagc
     4621 tgaagctgat cgtcgccggg ggctacgacg cccgcgagtg cctgccgctg ctcaagaagc
     4681 acgacgttcc catcattgtt ggcggcacct accgcctgcc gcggcggcgc ggcgacgcct
     4741 acgactcggc ctacgccctg ccggccgctt tgcacgcagc cggcatccgc tactgcatcg
     4801 gcacgcaggg ccgcttcggg gcgagcaacg tccggaacct gccttaccat gcggccgcgg
     4861 cgatcggttt cggattgccg gccgacgagg cgctcaaggc gatcacgctc tacccggcgc
     4921 agatcctcgg tgccagtgcc cgcgtcggct cgctggaggt cggcaaggac gccacgctca
     4981 tcatcgccga cggcgacgtg ctcgacacgc ccacgcaagt caccgccgcc tggattgcgg
     5041 gccgcaaggt cgaactcaac gaccgccaca agcggctcta tcgcaagtac gagcaaaaat
     5101 accagcagcc gtaggtcgcc ccgccggctt agaattgccg ctgtgaggcc attgcagctg
     5161 tccgtgctgg acctgtttgt gatgatgacg ggcggcgcgc tgctcgcgct ggccatcggc
     5221 ctgctgctgc ggagctagga tcgatcggtc gacaatcaag ctccgtcgga aatgaacgcc
     5281 gtgatcgtgc gcgctctgct acactgcagc catgctcaaa gtcgctggcg atgatccggg
     5341 cccttcgcgc aaagcctgga accgctcgac atccattggg cggagcccaa gccgtttccg
     5401 cagggaagca gccggtaatc gatgcccagg aaatcggagg tgcgattatg gaattcgatg
     5461 aggtcgcgaa caaagtcttg cagagcggcg gcgagctggt cgagttgaaa gccgacctgc
     5521 ggcaactcca ggcgcgggtg gatcggaata cgctggtcat ccaagtgctc aaggaaatgc
     5581 tgctcgcgca gggcggcttc cgcgaggagg aattcgccga tcgactccag cgggctgctg
     5641 cccaacggac ggccgacaag gcctgtcgca agtgcgggaa gccgctcaac cccaagcaca
     5701 accgctgcat gtattgcggc gaagggcgcc cgccggagct cctgtgagcg atccggcgat
     5761 ggaacccgac acggagcaat tggattgcgt tgctccgtag catgccaacg gcgagcgagg
     5821 cttaccagaa cttccaccac ggtttcttgg cggcctggcg gtcgcgctcg accaggcggt
     5881 tcagcgtggc caatgcggca tccatggttt gaccgctcac gccgcgctcg ggcacaacgc
     5941 acggaaacat cttcatgctg gagaggtccg gcttgtcgcc cgcggcgttc agcgcctgca
     6001 ccagcgcgtg cgattccgcg ttgtacaggc acagcgactg gaaggcgtct tcgctcatcg
     6061 catcgcgcaa ggaaacggca agtgcgcggg cccgggaata tgcgggaatg cccatcaggg
     6121 gtacgtcggt ctgaatgcgg ccgtcgcggc gggcgcggat gatggtcgag gaatactgca
     6181 cgcccaggtg ctcgaagagc atccggccga acgcgatcgt cgagatcgac tcggtctcct
     6241 gcacgaggtc cgggtcaaat ccctggccga ccatctccgc catgaggcga tcgaccgaat
     6301 cgtacttcgg gagatgcagc gcgaagtggc ggatcgtccg ctccagctcc gctcgccgct
     6361 gaatctccgt ggagttgcgg ggaaactcga acaccgcaaa ccgcgtcagg tatccgaagt
     6421 ccgggccgcg cacgccggcg ggccagtttg gatccaggtg ggacacgtcg tgcccgtcga
     6481 tttccaattc gcgatcccaa ccttccttcc ccttcgagac gatggtcacc ttcgccaggt
     6541 gcacccggcg cggggcagcg gattcggcag cgtagcggaa ccacggcttg cggtcgtcga
     6601 tggccgccat cacccgatcg tcctcgattc cgcgggacag cgagggtccg acaaaggccc
     6661 gcaggccggt cagcccatag tcgtttggcg aatagccccc cgcgaggtcg ccgccggcca
     6721 cgcccgcctg gcacgtcatg cagatgggct tgttgtcgag gaacgatttg atcggtccgg
     6781 caacgccggt gatccagatc gcggctgcct gccccagggc gccctcgcgg tcgtcgccca
     6841 ttccatacaa gcacgcgtcg agcgcttcgt cctcgtgttc gtgcagcgtg gttagcacgt
     6901 gcgcgtggac catgccgtcg gtggcggaag gaccggcgtc cttcaactcg gcgcggagcg
     6961 tcacccgccc ggcgaagagc tgcaagctgt cgtcactgcg gcgggtccag ccgcccaggt
     7021 cgccgccgtg cgcgaccacg ccctcttcga tccgttccag caggcgatct gagtctgaca
     7081 tcgagcgagc ccagtcattt cggcttgcgg cgaatctgga tctgtttcgc gtcccagccg
     7141 agttgtttgg cgacggactg cggatactcc cagcccgaat cgtcgtcctg gtagagcacc
     7201 accttcgacc gctcggcagc catggcgacg gcttgcggca tgtcgagata cctctcgacg
     7261 ttcaggaagt acggcccgtc gcggtgcgac ttgggcaggt cgtagaggtc gaggcgcttc
     7321 acgtctgatt cgaagagcga cgtgtagagc gtcacgccgg ccatcggtcc gtggctctgc
     7381 agccagagtg gcgcctcttt ccaggcggga atggactgcg aacatgtgca cgccaggtgc
     7441 acgtcccaca cgcgctctgc gtccaacgtc tggcctagca ggtagaagcg ccgctggttc
     7501 tgcacctgct tgcgggccga ctggtcccag gacgtcgctc ctattccgcg cggcgcgacg
     7561 tacatcatga cccaggcatt cttctgaagc atttgttgcg tcgatttcca gccctcggca
     7621 ttggtcgggc cagggtggtt gcggaacatt tccaggtcca catcgcgaaa ggtggccaga
     7681 agttcgctcc accgcgcttc gtccagaacg gtcagcacct tgaggtctgc cttttccagc
     7741 ccaaggcggt gcacgacgat caaccgaggc tgaatgcccc agccgcaatc gtaaacagaa
     7801 atgcggagac catcccggtc agccgcggcg acaaaatccg cgctgcgagg tgtgaacggc
     7861 tccggatcgg ccgagctggt ggtaaagtga atccttgaac cccggaacga tttttcctcc
     7921 agggccttca gccaaccctc gcgcatcttc tcccacgcgg ccttgtccgc gggaacttcc
     7981 ggcggcggcg ctttggcgac gaaggtctcg tgaatcttgg tattgatctg gtcatcgggc
     8041 agcttgccga agaccttgag ctgctcgggg gcgaagaatt tgggggctcg cgtgtcgatc
     8101 agcgaatcgt ctcccttgag ataatggttg aaccagcgga aggcgctggt ttgcagctcc
     8161 tgcgtgtcct tgtgcgggcc ggcggcgatg ttcagcgcga cctttgccgt cacgcccttc
     8221 tcgaccggct cgtgtagccg gtagatgcgg cggacctgct cgaacgtgcg atagaccccg
     8281 tccagcggaa agatgccgtc gctgtcgctg ttcgagatga gcagcggccg cggggccacc
     8341 agcgcagcga cttgcggata atcccagcgg taagtgttga gcaggtacat gcagtcgcag
     8401 tgcccttcga cgcagccgtc cacgacatgg ttttgcaggt cggtgatgcc ggcggtcggc
     8461 acggcgcact tgatccggtc gtcgagcgcc gcgatccacc agctgtacgc gccgccgccg
     8521 ctgcggccgg tcacgccgaa ccgcgactta tcgacttccg gccgcgtttc cagatagtcc
     8581 agggcccgca cgcagttcca ggcctcgacg cccgccggcg tgtagccgcg attgagccac
     8641 caccacatgc cgtagcgatg cgtgccgtgg tggatgcctt cgatctcgcc gagctgaagc
     8701 gtgtcgatcg tcaggcaggc gtagccgttc cgggcgaacc acgccccatg gtgctgatag
     8761 cccaccttcg cccccagcgg cactccgtcc ttggcattca cgctgtgacc gcagacatac
     8821 aggatcgtcg gcagcgactt gtcgaccttc ttcggcagat agaagttgcc ggtgacgtac
     8881 aaacccggcc gcgactggaa gtggagcttc tcgacgcgaa actcgtcctg ctccagcgtg
     8941 ccggtgatga ccggcttcaa gtccgtccgc tccggcagcg gatcgagccc caacatctcc
     9001 cgcagctgcc gccggcattc ttccttcttg gcgttccagt cgtcgagcgt tttgatctcg
     9061 gccaggcagt tcttctgaat ctgcgcagtt tccgctgcga agtacgcagc cagcatccgg
     9121 tcaccgcggc tggtgtccac tttcgccggg gccgtggcag cgtcctgggc agcggcccaa
     9181 ccaccaatca aaaccagtaa aacggctggc agcaggttct tcatggcgga gaatccggat
     9241 tgaggcgaga agtcggctaa tcgtcatccg ctgtagggcg gattgcaaca aggatcgagg
     9301 cttgtcaatt gtttaaacgg gcaaaggttt ggaaaaatag ttgacagggg ctgtgaaagg
     9361 tccctacgat taccgacatc tatcttggcg gcgattccgc tgcctgcgaa ctacgaagag
     9421 gcttgcgttt gggcgtcgaa gagtgggtgc tataaggggc actgccatgc gccggggacg
     9481 aactggtttt accctcgtcg agttgttggt tgttgtcgcg atcatcggaa ttctgatggc
     9541 gctgctcttg cctgccgtcc aggctgcgcg cgagtccgcg cgccgtacgc agtgctacaa
     9601 caacctcaag cagctggccc tggcctgcca caacttcgag cagaaccaca aaaagctgcc
     9661 gccctatttc ggcgtctttc ccgaacgagg cgtgggcgcg gtcgatggcg gctggttcct
     9721 gcacattctg ccctacatcg agcagcagca gatcaccgac gcggtgattg gggccggcgg
     9781 cggcatgggc cgcacgcagg ttctggtcac gccggccagc ccggattaca cgccgtccca
     9841 gacgatctat ccgccaggcg gccactggga aacggtggac ggcaccggcg gcgacggctc
     9901 cagccaccag gggcacacct ggccacacac cacgcctccg aaccgggttt gggtcggccc
     9961 gccgccgatc acgattcccg gcaccggcac gccgcccgtc tttgaattcg tgaatcacgg
    10021 gatcgacgtc atcgacagcg cgggcttcag cgtcctcaag tgctacagcg atcccagccg
    10081 cgcccagccg aaccaacgcg tgagcttccg cttcggccgc tggtcgctga cgaactatct
    10141 gggcaactat catttgttcg ccgtcaacgg cgtccgcacc aagcagcgcc ggctggcgga
    10201 agttcccgac ggcctttcca acaccgtgct cgcggcggaa ggcatgcggc tgtgcgacgg
    10261 cacgtaccgc ctggcgctgt ggggccacta tcagttccag cactcgcata atttcggcgt
    10321 cgattggaac ggcgtcccca acacgttcat gttccaggcc gtgccgcacc acacgaagtg
    10381 caacaactgg cgggtgcagg ggctgcactt cggccgcctc agcgtcgctt tcgccgacgg
    10441 cagcgtccgc tcgctcggca aggggatgac ccgccgcgaa gagagcgatc ccgacagccc
    10501 cgagtggggc atcgatcccg tcatcggctc cgagagcggc acctgggacc ggctgctcat
    10561 ggcggaagac ggcgaagcgc tcggcgatta ctagacggcg gggcatagag tttcttgggc
    10621 ggcgctggct tgcgcgcgaa gcgctgcctc cacttcttcc gcggattcgc cgccggcgag
    10681 cacggtggcg actggttcgc cggccgccag gtgctggccg atccgtggca gatcggcgat
    10741 gccgggaggc tctcccgctc ggttccactg gctgaccagc gcttcgaact ctcccgcaac
    10801 gacgctcgcg cggcgggcat agaccacggc cttgcctgca aaggtcgcaa cctgctggcg
    10861 aggctcgtct ggcagcgact gatgacggca ggcatccacg tgcagtcgga cgaagctcaa
    10921 tccaccgagc cgctccagga tctcgaccga cgccgaatac cggggattca cttccagcac
    10981 ccacaggccg tcgcgattgg cgagaaaatc gacgttgaac aagccctgca gctcaaaccg
    11041 cccggccagc agatttccca atcgcgtcag ccgctgagtc tggttgggcg gaagctccag
    11101 cggtgcgatc gaaccggcat agaggaacgg gcgatccagc ccaaagtcgc ggccggtgag
    11161 ctggcgcgac gtgcccagca gcaccgcctg cccccgcgcg gcaacaaaga cgctgctagc
    11221 cgactggcct tcgacgtatt cttgccagta ggtcgcccgc agcgtccgcc catagtcttc
    11281 tggcgcggcg aagcgaatcg actggccgcc gctggagcgc cgcggcttca agagccagac
    11341 attgccactc tggacgggtg gggcggcgcg ccactcggga aacaggcagc ccgtatcggt
    11401 gctgacggca gccaaacgcg caggatcgcg aactttccgc agcacccggc cgccatttcc
    11461 caagagcggc cgctcggccg ccagacgatc gaccagccgc gggtgattct ccaggccgcc
    11521 cgtgtagatc caaggcgaag gagttgcgct cgccagcgcg gcgggaaaat cggccggata
    11581 gcggtcgatc ttgacggccg ggcacatggc ggcaaggtcg cggtcggcaa acaggtcgat
    11641 cgcatagggc gcgatgcccg ctttcacggc cgaaaaggca gccgctcgcg tgctggcgcc
    11701 gagaatcgct aatctttcct gcatgttgtc ggaaaccgat tgacagcggc ggggcgcata
    11761 ggttctaacc aaaattcctc tcgcaactcc cgcgcatgag ccttttgctg gacgtaatga
    11821 gacgccggcc caggcccctt accgatcttg ccgacggcga tcgggtggtg ctctttccct
    11881 cgctggggca actgtcgacc gaccagcggc actggattgt tcagattcac ggcgacgtct
    11941 cggccgccgg ccacatgacg ctgggcaagc ggctgttgct caagctgctg cagcggacga
    12001 tgcgggccac gaacgacgac ttcgccagcc cgttgttcca gcagcggatc gcccggttcg
    12061 tcgcccagga ccggccggga aagcggatcg ccgtccggat cggcgaggcg gtcgtgccgc
    12121 tgcccaagac cacccgccgc aacggccact ttcaatcgct ggtcaaagtt ccctgcgagg
    12181 aagtcgcgga gagtggctcg ccggcagcac aggagttgcc cctggccgtg cattcgctga
    12241 cctgcgaagc gccgctgccg gggcgggcgt tcctgctcgc cccgcggggc ctctcggtga
    12301 tttcggacat cgacgacacg ctcaaacatt cgtttgtggc ctgcaagcgg acgctcctgg
    12361 ccaacacgtt cctcaggccg ttcgagacga tcccgggaat ggccgggctg tttcgcgatt
    12421 gggccgcggc cggcgccgcg atccactatg tctcctccag cccctggcaa ctctaccagc
    12481 acctggccga gcatctgcag gccgaaggct ttcccgatgg ctcgttccat ttgcgggcct
    12541 tccggctgcg ggaccacttg ctgcggcggc tattgatgct ccgccgcagc ggcaagatcg
    12601 gcgtcatccg caatatcttg cgaatgtacc cgcagcggcg gttcctgctc atcggcgatt
    12661 cgggcgagca cgacccggaa atttacggcg cgctggcccg ccgctttccg cagcaagtcg
    12721 ccggcgtttt gatccgccag ctcgacgacc cgcggagctc cccccagcgg tttaccaaag
    12781 ccttccgcca cgttgaccct ttgctggtgc ggctctatcg cgactccggc gaactctccg
    12841 ccgtgcgcct gcccgaaatg gcgtgacttc gttagcggcg gaatctcggc cgcaagcccc
    12901 agcggggcaa ggtaaggata attgcaccaa ccggcccagc cgcaacaacg ccccattggg
    12961 ccgcgcctcg gcccaacgcc ttgccgcgcc atgacttgag ttgaatcata acagcatatg
    13021 tggacatttg ggtttgaagc cttagagacc cctggcggga attatcctta cctccgcgct
    13081 ggtttgtctt tgggggcgcg tgtttccctt aggcgatgga cgaaagctcc gagtgttttc
    13141 agtcgggctg ccaaattgac cgctcgcgcg ccaactggaa cgatactgca aatatcgtac
    13201 caatgttcac tgatagccgc gcggcaacac tggccgcggc agcccgcgtg gattacagct
    13261 cggcgaaaac ttccccggcg gcttggatcg tcgcgtcgat gtgggcttcg gtgtgggcgg
    13321 cggagacgaa cagcgcttcg tactggctgc agggcatgta gacgccgcgg tcgatgaggc
    13381 cccagaagta gcgggcgtag cgggcggtgt cgctgcggct agcgccttcc caatcggcgg
    13441 gccgctcctg gttgaagaac agcgtgagca t
//
LOCUS       DASRJB010000502         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_989456, whole genome shotgun sequence.
ACCESSION   DASRJB010000502 DASRJB010000000
VERSION     DASRJB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989456"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFB80_13095"
     CDS             <1..1032
                     /locus_tag="VFB80_13095"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34755.1"
                     /translation="PLGVTDLTDLSSRYFLGVRLNCAQCHDHPFVAWKRQDYWGMAAF
                     FAQIQTPQRAKLVYMVGVQDNPKFTLATLPTADTIDGYQPEPPTFLGGQRLEAAGQRP
                     LRVALAEWMTAPENPFFARAAVNRMWWHFFGRGLVNPVDDMHSANVSSHPQLLDQLSR
                     EFAESGFDLKLLCRAIVGSRTYRQTSRPGQQGEGEERLFGRMPVKVLSAEQLYDSLIE
                     VLGPPAKAPAIDARLGTRYEFTQFFAADETGDPTSYQRGIPHLLRLMNSPQFAGRSIA
                     SLVSRTTAGRSVDEATDELFLATLARLPTAAERELARQQIAGAESSPTGYRELAWALL
                     LSSEFALNH"
     gene            1051..>2074
                     /locus_tag="VFB80_13100"
     CDS             1051..>2074
                     /locus_tag="VFB80_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34756.1"
                     /translation="MPTILARRNFLSLAAAGATLSGWLGRLAASAAEQPAAKSCILLW
                     MAGGPSHLDTFDLKPEASDKVRGEFRPIETSVPGIQISEHFPQLARQMQHAAVLRSMS
                     TVESDHQLATYHLHTGYQKRAGGIAFPSLGAIVARELGRADFPLPNFVCLGSGSRLGT
                     RSGFLGPDHQPLDVLDPARATDNLAPLASRAEFDRQYQLLRRLNASFDEAYRAEPARA
                     HASALARAVRLMNAEQRRAFDLSLEPEADRDRYGRGSFGQGCLVARRLVEAGVPFVEV
                     ISGGGVGWDTHQGNFPRTKALSLEADAAMASLLQDLHDRGRLDSTLVIWMGEFGRSPP
                     ITSGGGR"
BASE COUNT          333 a          700 c          689 g          352 t
ORIGIN      
        1 ccgcttgggg tcaccgacct gaccgacctc agctcgcgat acttcctcgg cgtacggctc
       61 aactgtgcgc agtgccacga ccaccccttc gtcgcctgga agcggcagga ctattggggc
      121 atggcggcgt tctttgccca gattcagacg cctcaacgcg cgaaattggt atatatggtt
      181 ggcgtgcaag acaatcccaa gtttacgctg gcgacgctgc cgaccgccga cacgatcgat
      241 ggctaccagc cagagccgcc gacttttctg ggcggccaaa ggctcgaggc cgccggccag
      301 cggccgctgc gagtcgcgct ggccgagtgg atgaccgcgc cggagaaccc gtttttcgcc
      361 cgagcggcgg tcaaccggat gtggtggcac ttcttcgggc ggggactcgt caatccggtg
      421 gacgacatgc actcggccaa cgtctcgtcg cacccgcagc tgctggacca actcagccgg
      481 gaattcgccg aatcgggctt cgatctgaaa ctgctttgcc gtgcgatcgt cggcagccgc
      541 acgtaccggc aaaccagccg ccccggtcag cagggcgaag gggaggagcg gctcttcggc
      601 cgaatgccgg tcaaggtgct gtcggccgag cagctctacg actcgctgat tgaggtgctg
      661 gggcctcccg cgaaagcgcc ggctatcgac gcgcggttgg gcacgcggta cgaattcacg
      721 cagttctttg ccgccgacga aaccggcgat ccgacgagct atcagcgggg gattccgcac
      781 ctgttgcggc tgatgaactc gccccagttc gccggccgca gcatcgcctc gcttgtctcg
      841 cggacgaccg ccggccgctc ggtggacgaa gcgaccgacg aattgttcct cgcgacgctg
      901 gccaggcttc ctactgcggc cgagcgcgag ctcgcgcggc agcaaatagc cggcgccgaa
      961 agttccccaa ccggctatcg ggagctggcc tgggcactgt tgctctctag cgagttcgca
     1021 ctcaatcatt aaccacgcgg caaatcgcct atgcctacca tccttgcgcg tcgcaatttc
     1081 ctatcgctgg ccgcggccgg cgctacgctc agcggctggc tgggccggct ggcggcaagc
     1141 gcggccgagc agccggcggc caaatcgtgc attctccttt ggatggcggg cgggccgagc
     1201 cacctcgata ccttcgatct caagcccgag gcctcggaca aggttcgcgg cgaattcagg
     1261 ccgatcgaaa cctccgtccc gggaattcag atcagcgaac actttcccca gctcgcccgg
     1321 cagatgcagc atgccgccgt gctccgcagc atgagcacgg tcgagtcgga ccaccagttg
     1381 gccacgtatc acctgcatac tggctatcag aaacgggccg gcggaatcgc gttcccgagc
     1441 ctgggggcga tcgtcgcgcg cgagctgggc cgggcggact ttccgctgcc gaatttcgtc
     1501 tgcttggggt ccggcagccg gctcggcacg cgctccggct tcctcggccc ggaccatcag
     1561 ccgctggacg tgctcgatcc ggcgcgggcg acggacaatc tcgcgccgct ggcaagtcgc
     1621 gcggagtttg accggcagta tcaattgctg cggcggctga acgcctcgtt cgacgaggcc
     1681 taccgcgcgg agccggcgcg agcccacgct tcggcgctgg cgcgggcggt gcgcctgatg
     1741 aatgccgaac agcggcgggc cttcgacttg tcgctcgaac cggaggccga ccgcgatcgc
     1801 tacggccgcg gcagttttgg gcaaggctgt ctggtggccc gccggctggt cgaggcgggc
     1861 gtgccgtttg tcgaggtgat cagcggcggc ggcgtgggct gggacacgca ccagggaaac
     1921 tttcctcgca ccaaggccct ctcgctcgag gccgacgcgg cgatggcgtc gctcctccag
     1981 gatttgcacg accgcggccg gctcgattcg acgctggtaa tctggatggg agaattcggc
     2041 cgctcgccgc caatcaccag cggcggcggc cgca
//
LOCUS       DASRJB010000503         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_139960, whole genome shotgun sequence.
ACCESSION   DASRJB010000503 DASRJB010000000
VERSION     DASRJB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139960"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFB80_13105"
     CDS             <1..336
                     /locus_tag="VFB80_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN34757.1"
                     /translation="ARDLVQLFKLLADETRLRIMYYLMQKDELNVRTLCRMLRQSQPA
                     VSHHLALLRVAGIIECRRDGKHNYYHLVPKRCQAYLNVVFGVSGDQTRKVRVEDAVLT
                     YGRDAQLVS"
     gene            352..825
                     /locus_tag="VFB80_13110"
     CDS             352..825
                     /locus_tag="VFB80_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34758.1"
                     /translation="MFLFGDHPVQESPFAYSIDLANLLTAQLTKFTTLNRHQLAGHVA
                     NLDFWLAEARHALEVLDGYQVRFDRLKAAQTQYASCHQTTEFSHDDPCCTKQHASVPK
                     RIPGDERSRPRRQLCDAVYRFLVRCFRERLIDETRLLEACESLKISVESRDLNLR"
     gene            896..1978
                     /locus_tag="VFB80_13115"
     CDS             896..1978
                     /locus_tag="VFB80_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34759.1"
                     /translation="MRNLIFALLAGSLSLAAAAEPRKTAVSIAADAFHINGRPTYAGR
                     TWQGHKIEGLLLNSRMVQGIFDDLNPEPVKLWAYPDTGKWDAERNTREFIAAMPTWRE
                     HGLLAFTICLQGGSPQGYSRDQPWHNSAINADGSLRPDYMARLERIVNRADELGMVVI
                     LGVFYFGQDQKIADEAAVKRALDNAVTWVHDRGYTNVLIEINNECNVRYDHPILQPAR
                     VHELIEQVKSRTRDGRRLLVGTSYGGSTLPKENVVRASDFLLIHGNGVKEPTRIAEMV
                     RQCRQVPGYRAMPILFNEDDHFDFDKPDYNFKAALSEYASWGYFDYRMQGEAYGDGYQ
                     SVPVNWGLSSPRKKAFFERLKEVTGN"
     gene            complement(1986..2738)
                     /locus_tag="VFB80_13120"
     CDS             complement(1986..2738)
                     /locus_tag="VFB80_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZN34760.1"
                     /translation="MHLGNGAITPECVVLTVSAGAAGLAAAAAAARADLAKRDKLLLA
                     AGLGALVFAAQAVNVPAWAGVSAHLVGGVLLASLLGPGLGACTMALVLAVQACLLGDG
                     GVAALGANIVNMALLPAALVALAKGRSTIVMALLAGLAVPLAAGLIAIETALFRPAAE
                     LAGWSSFAALLIGTHLWIGVLEAGCTAGVIAALSRLTEPAAMQPRYALRTAAWCLAAA
                     LVLIAIWPLSSGLPDGYEASALASGQERLLAD"
     gene            2885..4366
                     /locus_tag="VFB80_13125"
     CDS             2885..4366
                     /locus_tag="VFB80_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZN34761.1"
                     /translation="MRIGIIALLQESNTFIGQPTTFAHFEQDLLATGAAVRERLAGTH
                     HEVAGMFHALDEAGEEAVPIFAARAYPFGVVEAAATQRLLDLLFAELKKAGPLDGVLV
                     APHGATVSEPFPDLDGHWLSELRKHVGDDVPIIGTLDPHANLSPAMVAAATALIAYRT
                     NPHLDQRARGIDAARMMIETVRGKLRPTMAAAFPPLAINIEKQHTAEEPCHSFYRFAD
                     QQLLDDHLLVNSIILGFPYADVAEMGSATIAVTHDNPALARRLADELADYLWERREQF
                     AGDFIGIDEALDKAAKMSGPVCLLDMGDNVGGGSPGDGTLLALAMHERKLPKSFVCLC
                     DAEAVAQALAMGEWGTGTFHVGGKLDRLHGPPLVCEATVVGLFDGQFEEPQPRHGGFT
                     RFDQGPTAVIRADHGLTIMLTSRRAPPFSLRQLTAHGVLPEQQHLLVAKGVNAPVAAY
                     KEVCKQFIRVNTPGVTTADVEQLHYQHRRKPMFPWERDAVWRE"
     gene            complement(4438..5097)
                     /locus_tag="VFB80_13130"
     CDS             complement(4438..5097)
                     /locus_tag="VFB80_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN34762.1"
                     /translation="MTRIVATVAALAILSGPLYAQAPKAAPAADAAKEKWESLFDGKS
                     LTGWKETNFGGEGEIEVKDGQIILQMGQPLTGLTIKDGSKLPKDNYEITLQAMKRKGD
                     DFFCALTFPIRDTHASFVVGGWGGSVVGLSSIDGQDASENETTDYVTLPKDKWHTIKV
                     RVAGGKVECWLNDKQQVEVELKDKRLSTRIEVDANKPLGICCYNVEAAVKDIKLRRLE
                     K"
BASE COUNT          923 a         1802 c         1604 g          856 t
ORIGIN      
        1 gcccgcgacc tggtccagct cttcaagctg ctggccgacg aaacgcggct gcggatcatg
       61 tattacctga tgcaaaagga cgagctgaac gtgcggacgc tctgccgcat gctgcggcaa
      121 agccagccgg cggtgagcca ccacctggcc ctgctgcggg tggcgggcat catcgaatgc
      181 cgccgcgacg gcaagcacaa ctactatcac ctcgtgccca agcggtgcca ggcctacctg
      241 aacgtcgtgt tcggcgtcag cggcgaccag acccgcaagg tccgcgtcga agacgccgtc
      301 ctcacctacg gccgcgacgc ccagctcgtc tcgtagaatt ggcgtcctcg cttgttcttg
      361 ttcggtgacc atcctgtcca ggagtctccc ttcgcctatt ccatcgacct tgccaacctg
      421 ctcaccgccc agctgaccaa attcacaacg ctcaatcgcc atcaactggc cggccacgtc
      481 gccaatctcg atttctggct cgctgaagcg cggcacgccc tggaggtgct cgacggctat
      541 caagtcaggt ttgatcgcct aaaggccgct cagacgcagt acgccagctg ccaccagacg
      601 actgagttct cgcacgacga tccctgctgc accaaacagc acgcctccgt tccgaagcga
      661 attcccggcg acgagcgcag ccgacctcgc cgtcaattgt gcgatgccgt ctatcgcttc
      721 ctcgtacgct gcttccgcga gcggttaatc gacgagaccc ggctgctaga ggcctgcgaa
      781 tcactcaaga tcagcgtgga atcgcgagat ctgaatttgc gatagccgcg cagttcaatt
      841 cgccgtccgt gcggcgtaca ttgggcgagc cactgtcgct cactgaggaa tttccatgcg
      901 caatctcatt ttcgcactgc tggcgggatc gttgtccttg gcagccgctg ccgagccgcg
      961 caaaaccgcc gtctcgattg ccgcagacgc cttccacatc aacggccggc caacctacgc
     1021 cggccgcacc tggcaggggc acaagatcga aggcctgctg ctcaacagcc gcatggtgca
     1081 gggcatcttc gatgacctca atcccgagcc ggtcaagctc tgggcctatc ccgatacggg
     1141 caagtgggac gccgagcgca acacgcggga gtttatcgcg gcgatgccga cttggcgcga
     1201 gcacggcctc ttggccttca cgatctgcct gcagggcggc agcccgcagg gctattcccg
     1261 cgatcagccg tggcacaatt cggcgatcaa cgccgatggc agcttgcggc cggactacat
     1321 ggcccgcctg gagcggatcg tcaaccgcgc cgacgagctg ggcatggtcg tcatcctcgg
     1381 cgtcttctat ttcggccagg accagaagat cgccgatgaa gcggccgtca agcgcgccct
     1441 ggataacgcc gtgacctggg tccatgaccg cggctacacc aacgtcctga tcgaaatcaa
     1501 caacgagtgc aacgtccgct acgaccatcc gatcctgcag cccgcccgcg ttcacgagct
     1561 gatcgagcag gtcaagagcc gcacgcggga cggccggcgg ctgctggtcg gcacgagcta
     1621 cggcggcagc acgcttccca aggaaaacgt ggtccgcgcg tcggactttc tgctgatcca
     1681 cggcaacgga gtcaaagagc ccacgcggat cgccgagatg gtccgccagt gccggcaggt
     1741 gcccggctac cgcgcgatgc cgatcctgtt caacgaggac gaccatttcg acttcgacaa
     1801 gcccgactac aacttcaagg ctgccctcag cgaatacgcc tcctggggct attttgacta
     1861 tcgcatgcag ggcgaagcgt atggcgacgg ctaccagagc gtgcccgtca actggggcct
     1921 ctccagcccg cgcaaaaagg cattcttcga gcggctcaaa gaggtcactg gcaactagcg
     1981 tttcgctaat ccgccagcag gcgctcttga ccgctggcca gcgccgaggc ctcgtagccg
     2041 tcgggcaagc ccgacgacag cggccatatc gcgattagca ccagcgcggc ggccaggcac
     2101 caggcggcgg tccgcagcgc gtaccgcggc tgcatcgccg ccggttcggt cagccgcgac
     2161 agagcggcaa tcacccccgc cgtgcacccc gcctccagta ctccgatcca cagatgcgtg
     2221 ccaatcaaga gcgcggcgaa gctcgaccag ccggccagtt cggcggccgg gcgaaacagc
     2281 gccgtctcga tcgcgatcag cccggcggcg agcggcacgg ccagtccggc cagcagggcc
     2341 atcacgatcg tggagcggcc tttggccagc gcgaccagcg cggccggcaa cagggccatg
     2401 ttgacaatgt tagcgcccag cgcggccact ccgccgtcgc cgagcagaca tgcctgcacg
     2461 gctaacacca acgccatcgt gcaggcgccg agtcctggcc ccagcaggct cgccagcaaa
     2521 acgccgccga cgaggtgcgc actgacgccc gcccacgcgg gcacgttcac cgcctgcgcg
     2581 gcaaacacaa gtgcgcccag tcccgccgcc agcagcagct tgtcgcgctt cgccaggtcc
     2641 gcgcgggcgg ccgccgcagc agccgccagg ccagccgcac cggcagaaac cgtgaggacg
     2701 acgcattccg gcgtgattgc tccatttccg aggtgcatat tcaagctcca cgtccgctgg
     2761 tggtgaaagt cccggcaagt gtaccccatc cgccgcggcg ggcgaagcgg agcaggtggc
     2821 cggttcgggg gaaaactgat accctcagat tccggttctt cctcgccgct aacgcctgct
     2881 ttccatgcgg atcggcatca tcgccctgct gcaggaatcg aacaccttca tcgggcagcc
     2941 gacgacgttt gcgcattttg agcaggattt gctggcgacg ggagcggcgg tccgcgagcg
     3001 cctggcgggc acgcaccacg aagtcgccgg catgttccac gccctggacg aggcgggcga
     3061 ggaggccgtg ccgatcttcg ccgcccgggc ctatccgttc ggcgtcgtcg aggccgcggc
     3121 gacccagcgg ctgctggacc tcctgttcgc tgagctgaaa aaggcgggcc cgctcgacgg
     3181 cgtgctcgtc gcgccgcacg gagcgacggt cagcgagccg tttcccgacc tcgacggcca
     3241 ctggctgagc gagctgcgga aacacgtcgg cgacgacgtg ccgatcatcg ggacgctcga
     3301 cccgcacgcc aacctctcgc cggcaatggt tgcggcggcg actgccctga tcgcctatcg
     3361 cacgaacccg cacctggacc agcgggcccg cggcatcgac gccgcccgga tgatgatcga
     3421 gaccgtccgc ggcaagctgc ggccgacgat ggccgcggcg tttccgccgc tggcgatcaa
     3481 catcgaaaag cagcataccg cggaggagcc ctgccactcg ttctatcgct tcgccgacca
     3541 gcagctgctc gacgaccacc tgctcgtcaa cagcattatc ctcggcttcc cgtacgccga
     3601 cgtggccgag atgggctcgg cgacgatcgc cgtcacgcac gacaacccgg ccctggcccg
     3661 ccggctggct gacgagctgg ccgactacct ctgggagcgc cgcgagcaat tcgccggcga
     3721 cttcatcggc atcgacgagg cgctcgacaa ggccgcgaag atgtcggggc cggtctgcct
     3781 gctcgacatg ggcgacaacg tcggcggcgg ttcgcccggc gacggcacgc tgctggccct
     3841 ggcgatgcac gagcgaaagc tgcccaaatc gttcgtctgt ttgtgcgacg ccgaagcggt
     3901 cgcccaggcc ctggccatgg gcgaatgggg cacgggcacg tttcacgtcg gcggcaagtt
     3961 ggatcggctg cacgggccac cgctggtttg cgaggcgacg gtcgtgggac tcttcgacgg
     4021 gcagttcgag gagccacagc cgcggcacgg cggcttcacg cgttttgacc agggcccgac
     4081 ggccgtgatc cgcgccgacc acgggctgac gatcatgctc accagccggc gagcgccgcc
     4141 gttcagcctg cggcagctga cggcccacgg cgttctgccg gagcagcagc acttgctcgt
     4201 cgccaagggc gtcaacgcgc cggtcgctgc ttacaaggaa gtctgcaaac agttcatccg
     4261 cgtcaacaca ccgggcgtca cgaccgccga cgtcgagcag ctccattacc agcaccgccg
     4321 caagccgatg ttcccgtggg agcgggatgc ggtctggcgg gagtgaccaa agccgcgacc
     4381 attggtcgcg gttcgcgcgt cgccaccgga ccgagaccaa tggtcgcggc tttgaggcta
     4441 tttctccagc cggcgcagct tgatgtcctt cacggcggcc tcgacgttgt agcagcagat
     4501 gccgagcggc ttgttggcat cgacctcgat gcgggtggag agccgcttgt ctttcaattc
     4561 gacttcgacc tgctgcttgt cgttcagcca gcactcgacc ttgccgccgg caacgcggac
     4621 cttgatcgtg tgccacttgt ccttggggag cgtgacgtag tcggtcgttt cgttctccga
     4681 agcgtcctgg ccgtcgatgc tcgacaggcc gacgaccgaa ccgccccagc cgccgacgac
     4741 gaagctggcg tgcgtgtcgc ggatcggaaa ggtgagggcg cagaagaaat cgtccccctt
     4801 ccgcttcatc gcctggagcg tgatttcgta attgtccttg ggcagcttac tgccgtcctt
     4861 gatcgtcagc ccggtcagcg gctgccccat ctgcaggatg atctgtccgt ccttgacctc
     4921 gatctcacct tcgccaccga aattcgtctc cttccagccc gtcagggact tgccgtcgaa
     4981 caagctctcc cacttttcct tggcggcatc agccgcgggc gctgccttcg gagcctgggc
     5041 atatagcggc ccgctgagga tcgccagagc ggcaacggtc gcgacgattc gagtcatggc
     5101 acttctccgg acagggacgg caaaaccaaa ccaacatcct gggcgaccgc tggctgaaat
     5161 tcaacgggca cctatgcgcg catag
//
LOCUS       DASRJB010000504         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2678809, whole genome shotgun sequence.
ACCESSION   DASRJB010000504 DASRJB010000000
VERSION     DASRJB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678809"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFB80_13135"
     CDS             <1..1159
                     /locus_tag="VFB80_13135"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN34763.1"
                     /translation="RDSASAVALVTGQEDADKEASSLDYAALANFPGTLVFYMGVTTA
                     GHWTQALIAAGKSSATPVAIVRRVSLPGQQRLDTTLGEVADVVKDQHLRPPVVFIVGD
                     VAANGPAWSWFERRPLFGQKVLVTRPAHQSDDLAQPLAEMGAAVLLQPVIKIYPPGVR
                     SHIEPEFLDDLVKRLPLHGRWEHVDRALELLDRQDWLVFSSSNGVRYLLSRLPAIGKD
                     ARWLDNVKIAAIGSATADELAKFHLRPDLVPDEFRAEALAAALAGGAAGKRFLLVRAS
                     RGREVLADELTKAGGIVEQVVAYESVDVESADPEIAAQMADGKIDWTTVTSSAIARSL
                     ARLFGESLRRTKLVSISPVTSATLRELGFEPAAEAKEYTMAGVVAAIEGHA"
     gene            complement(1876..3282)
                     /locus_tag="VFB80_13140"
     CDS             complement(1876..3282)
                     /locus_tag="VFB80_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34764.1"
                     /translation="MTTARFGATLALCAILSIAAAGFAVAGDDRLVVHEWGTFTSLQD
                     EQGRELSGINTDDEPVPKFVHNLNQFLLSRPVLSSLHWQYRQKGAPRVHPQITMRLET
                     PVLYFYPPKSWKEGRPIDVSVRFRGGWLTEFYPNAQAQAPGLKEGIFEFGALNRQTES
                     SLSWNDLRIGTQGAGPDTDAHVWLAPRKVAAANITTPDNESERYLFYRGVGNLRAPLR
                     ATLDRKAGRLSLAANFDEVPSSRVPPQIPVLWLVQVRPDGVCAFRTLEGFSVSGDVSA
                     KLAETSYKFDSSEFREGNRDQLEAAMHAALVADGLYPDEATALLTTWQRSYFTSPGLR
                     LFYLVPRVWTDHYLPLTLSGEAEVNRVMIGRLELISDEQRTLLGKLADVTPSDGRWVD
                     LIPESAARERFLAGRTDFGDLGTKIPADYQIYLDLGRFRNALVAHEERVRPAPSLTKF
                     INTYELHPFRVPAVQASP"
     gene            complement(3372..4532)
                     /locus_tag="VFB80_13145"
     CDS             complement(3372..4532)
                     /locus_tag="VFB80_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN34765.1"
                     /translation="MRANPTVVKLFCFLALLGSFPAAAADQPLLALAAGSPLNVGGGA
                     LAAGDVNGDRCADLIVVAGASLQIYFGSATRPWQPKPDVTNSLPGKASEVAVVDLDGD
                     AKPDLVLADHDSYGVTVLRGSGDGKFQPCPGSPFAAREGTQPHTHGLVVTDLDQDGRL
                     DIVTANNSDGDVSVLLGQPGLIFARARGSPFSCGKSPYPIACADINGDGRADILVPNA
                     VHDSAAVKSLTILLAKSGGELARAPSSPLVPDATVWYVAAGDLNGDRHPDVAATHSEG
                     GSGVTIWLNDGQGKLSPAAGSPLELGHGAWGVEIADMNRDGHGDLVLGADESIRVFLG
                     DARGRFRPAGGSPFKTGKGAWRIVVADFNGDSKLDVATWCVEAKRLEVWQAN"
     gene            4710..5648
                     /locus_tag="VFB80_13150"
     CDS             4710..5648
                     /locus_tag="VFB80_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN34766.1"
                     /translation="MHPTEPLPSLSTDQVAAFVQLARLGSLRAAAETLFITEQGLRSR
                     LLALEGRLGVELYRKARGLRRRTPLTPQGQQFLPHAEAFLDRAAHLCGMFRETEEQQE
                     VQVVASQYLIAYLLIDTVRRFHRMFPQIHVRLSARTEHEIEETLLESLEFSFGVAAPY
                     ESSPELTYRHLFSMHWSLIAPPRHPLAQKRSLRLADVVPHPLIMFERGSTGRQHVAEA
                     FQRLGLSPHVELEATTTDLIVRMVEAGLGVAIVPLHPSGAVTRGRKVAIQGLGKQVRP
                     IDSGVLLRRNEQLPPAAEKLLDFLCATQSAASPNSP"
     gene            5758..>8124
                     /locus_tag="VFB80_13155"
     CDS             5758..>8124
                     /locus_tag="VFB80_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34767.1"
                     /translation="MRWCCWLAATLSLSLSLTGRLAAQTAPPDPAAESPKPIAGPAVE
                     ASPLDTFLLRDSKGNLVPVPGMPFEEFERLLLMKKGLSPPAAPGFTFDALSLVGEAKE
                     KLATFEVTATIRVRAEGWIRVPLLLPSAILRGQSRHEGPGEHFVAFDSAAGGYVCWLK
                     GADARPHVVTLEFAAGLSTAGDDTRLALSLPRATESSLRLTVPVASIEAALTGEGLAT
                     TRSAGGAQSEISVLGPAGELQLSWHKGAAAVKGPTQLDASGSEIQVRVESQQSITSTA
                     RLKVRSYGGLLETFQVRLPPGMELAPSALGPVGYTLTPLAGSGPAPAPRRNGPGLVVE
                     VRLDKPTPGVADVQLAASLVPEASPAPLMPARFEVLGAVRQRGTIDVSVGGQWQLEWT
                     EDRSVRRVDLAAETSPADLVARFDYFRQPCGLQLRVAARPSRVSVEPTHLVFVDPQLV
                     LIKSTLKYRFRGSRVTALALELGDWKFGSIMPSDLFEAPVFKADAAGLVQLPFRQDKP
                     LPAELELTLEAHRQLAPAAEHLVFGLPRPLADIVAPATLVIAPADNVELTPQEELLLG
                     LTPDTSGVRIPERQQPPLVYRDLGGGEPALFATRYRTRTRTSTVVARATARIDRQQLQ
                     IEQQLEYRIAHEPQRAFTLLAPRGLLSSGNLQVTLGGQPLSTSAAPEPLAPAATSAQS
                     APGEAVQRIEFTTPSDQIGVCTIVVRYTSALPASDGHQPLDVSVPLVIPADEGEQQFQ
                     AQTLEFLAGEAWQIEPGEHTGGEFSHPAPLPAAAGGQAFTWSRATPLTH"
BASE COUNT         1365 a         2670 c         2683 g         1406 t
ORIGIN      
        1 tcgcgacagt gcgtcggccg tggccctcgt caccggtcag gaagatgcgg acaaggaagc
       61 ttcgtcattg gactacgccg ccttggcgaa tttcccaggc acgctcgtct tctacatggg
      121 tgtgacgaca gccggccact ggacccaggc gctgatcgcc gcgggcaaat cgtccgccac
      181 acccgtcgcg atcgtgcgcc gggtcagcct gccgggccaa cagcggctcg acacgacgct
      241 cggcgaagtc gcagacgtcg ttaaggacca gcacttgcgg ccgccggtcg tattcatcgt
      301 cggcgacgtc gccgccaacg gaccggcctg gtcctggttc gaacgccggc cgctgttcgg
      361 ccagaaagtc ctcgtcactc ggccggcgca tcagtccgac gacctggccc agccgctggc
      421 ggaaatgggc gccgccgtcc tgctgcaacc ggttattaag atctatccgc cgggggttcg
      481 gtcacatatc gagccggaat ttctggacga cctggtcaaa cggttgccgc tgcacggtcg
      541 ctgggaacac gttgaccgcg cactggagct gctcgaccgt caggactggc tggtcttttc
      601 cagcagcaac ggtgtgcgct atttgctcag ccgcctgccg gcgatcggca aggacgcgcg
      661 ctggctggac aacgtcaaga tcgcggccat cggatcggcg accgcggacg agctggcgaa
      721 attccatctc cggccggacc tggttcccga cgaattccgc gccgaggcgc tggccgcggc
      781 cctggccggc ggagcagccg ggaagcggtt tctgctggtt cgcgccagcc gggggcgcga
      841 ggtgctggcg gacgagctga ccaaagcggg cggaatagtc gagcaggttg tggcctacga
      901 gagcgtcgat gtcgaatcgg ctgatccgga aatcgccgcg caaatggcgg acgggaagat
      961 cgattggacg accgtcacca gctcggcgat cgcccgctcg ctggcccggc tgttcggcga
     1021 gtcgctgcgg cggacgaagc tggtcagcat cagcccggtg acatcggcca cgctccgcga
     1081 actgggcttc gagccggcgg cagaggccaa agagtacacg atggccggcg tcgtggccgc
     1141 catcgaaggg cacgcgtagc gggagcaata tcaggccccc tggtcggatg gtgcgcaacg
     1201 cgcctccttt ccattttccc gatgtaactg ctgacgcctc attggcttac ggcgagcagg
     1261 ctgaaacacg ataagtagta cttattagtg tttgagggac gctgtccgaa cttcggaacc
     1321 aagttgacct cacgctccgc gtgaggcgaa catctcgcgg agcgagatga ctactatttt
     1381 gaacggtcgg ggtcgggagt ctttggcgcc gcacgacttg ccatgggcaa cggacccgcc
     1441 gccaaagact cccgacccct cgcgctgcca ctcggttcga caaccaccgc caagctgtct
     1501 ctacccggca ccgcgtggcg ggacgaatcg cgggccaccc ccaaggggtc aggagtcttt
     1561 ggcggtcgtg gtcaccatat ggagaacatg attgcgccaa agactcccga ccccggaggt
     1621 cgttcggcag ccgctggaaa aacatgtgtt cagtgggtgg cgaccctgat tgtcacatgt
     1681 ggacagatta tacaatacgc tcccgcgttt ttctctgccg cgtagccaaa attaccgaaa
     1741 agcggccgcg caagtctttg gcccgcttgg ctttgcaact attttcaaga aaatgcgtgc
     1801 cgcgctgggc tttctccagg cggcaactgg cagattgccg ctggaaacca cgattgggcg
     1861 actggaaagc cgccgctacg gagacgcctg cactgcgggc acgcggaacg ggtgcagctc
     1921 gtaggtattg atgaacttcg tcaagctggg cgccggtcgc acgcgttcct cgtgggccac
     1981 cagcgcgttg cggaagcggc caagatccaa gtagatctgg tagtcggccg gaatcttggt
     2041 gcccagatcg ccaaagtcgg tgcggccggc caggaaccgc tcgcgagcgg ccgattcagg
     2101 gatcaaatct acccagcggc cgtccgacgg agtgacatcc gctaatttgc cgagcaacgt
     2161 ccgctgctcg tcgctgatca actccagccg gccgatcatg acgcgattga cctcggcttc
     2221 gcccgagagc gtcagcggca ggtagtgatc ggtccagacc cgcggcacga ggtagaacag
     2281 ccgcaggccg gggctggtga agtacgacct ctgccaggtc gtgagcaagg cggtggcttc
     2341 gtcggggtac agcccgtcgg cgaccagcgc ggcgtgcatg gcggcttcga gctgatcgcg
     2401 attgccctcg cggaactcgc tggaatcgaa cttatagctg gtctcggcca gtttcgcaga
     2461 gacgtcgccg ctgacgctga agccttcgag cgtgcggaag gcgcaaaccc cgtcgggccg
     2521 cacctgcacg agccacagca cgggaatctg cggcggcacg cggctgctgg gaacttcgtc
     2581 gaaattcgcc gcgagcgaca gccggccagc cttgcggtcc agcgtcgcgc ggagcggcgc
     2641 gcgcaagttg cccacgccgc ggtagaacag gtagcgttcg ctttcgttgt cgggcgtggt
     2701 gatgttggcc gctgcaacct tgcgcggcgc gagccagaca tgcgcgtcgg tatccgggcc
     2761 ggcgccctga gtgccgatcc gcagatcgtt ccagctaaga ctgctctcgg tctgccggtt
     2821 cagcgcgccg aattcgaata tcccttcctt caggccgggc gcttgggcct gggcattggg
     2881 atagaactcc gtgagccagc cgccgcggaa gcggaccgag acgtcgatgg gccgcccctc
     2941 tttccaggac ttcggcggat agaaatagag cacgggcgtc tccagccgca tcgtgatttg
     3001 cggatgcacg cgcggcgcgc ccttctggcg atactgccag tgcaagctcg acagcacggg
     3061 ccggctgagc aggaactggt tgaggttgtg aacgaatttg ggcacgggct cgtcgtcggt
     3121 gttgatgccc gacagctccc ggccctgctc gtcctgcagc gacgtaaacg tgccccattc
     3181 gtgaacgacc aggcggtcgt cgccggcgac cgcgaagccg gccgcggcga ttgacagaat
     3241 tgcgcacagg gcgagcgtcg caccaaatcg tgcagttgtc atgttcgaac tcctcagggg
     3301 actatgggcg aaattggccg ccagccttcg gctggaggga aatctgcgtc gtgcacgaaa
     3361 actcctcgag actagtttgc ctgccagacc tccaagcgtt tggcttctac gcaccaggtc
     3421 gccacgtcga gcttgctgtc gccgttaaaa tcggccacga caattcgcca ggcgcctttg
     3481 ccagtcttaa agggcgagcc gcccgccggc cgaaaccgtc cgcgagcgtc tcccaggaaa
     3541 actcggatgc tctcgtccgc gccgagaacc aggtcgccgt gcccgtcgcg attcatgtcc
     3601 gcgatttcga cgccccacgc gccgtgaccg agctcgagtg gagagccggc cgcgggcgac
     3661 agctttcctt ggccgtcatt gagccagatc gtgaccccgc tgccgccttc ggaatgcgtc
     3721 gccgcgacgt cgggatgccg atcgccgttc aggtcgcctg ccgcgacgta ccacacggtg
     3781 gcatcgggga ccagcggcga cgacggcgcc cttgccagtt caccgccgct cttggccagc
     3841 aggatcgtaa gcgacttgac ggctgccgag tcgtgcactg cattgggaac cagaatgtcc
     3901 gcgcggccat cgccgttgat gtcggcgcac gcaatgggat aagggctctt gccgcaggaa
     3961 aagggcgaac cgcgagctcg cgcgaagatt aatcccggct ggcccaagag caccgacaca
     4021 tctccgtcgc tgttgttggc cgtgacaatg tccagccggc cgtcctggtc taagtcggtg
     4081 acgacgagcc catgcgtgtg cggctgtgtt ccctcgcgtg cggcaaaagg cgagccaggg
     4141 cagggctgga acttgccgtc gccgcttccc cgcaggacgg tgacgccgta gctgtcgtgg
     4201 tcggcaagca ccagatccgg cttcgcgtcg ccatccaggt ccaccaccgc gacctcgctc
     4261 gccttgcctg gcagcgaatt ggtgacgtcc ggtttcggct gccaggggcg cgtcgcgctg
     4321 ccgaagtaga tctgcaggct cgcgccggcc acgacgatca gatcggcgca gcggtcgccg
     4381 ttgacgtcac ctgcagctaa tgcgcccccg ccgacgttca gcggcgagcc ggccgccagt
     4441 gcgagcagcg gctgatcggc cgctgcagcg ggaaatgaac ccagcagcgc gagaaagcag
     4501 aacagtttca ccaccgtagg gttggctcgc ataagctcgc ctctctgtcc agcccgcctg
     4561 cgcgacgggg cgctcgccct tgggccactt gcgttcattg tcggacttgc ttgatcgcgg
     4621 gaaaaattgt ttttattctg cgacgtaatg gttattgttg tctttggaag tgccgccggg
     4681 gtggtagcct gccttggcag gagcggatga tgcacccaac cgaacccttg ccgtcgctct
     4741 cgaccgatca ggtcgccgcc ttcgtgcagc tcgcccgcct gggcagcttg cgggccgcgg
     4801 ccgagaccct gttcatcacc gagcagggcc tgcgcagccg gctgctggcg ctcgaagggc
     4861 ggctgggcgt ggagctgtac cgcaaggccc gcgggctgcg ccgccgcacg ccgctgacgc
     4921 cgcaggggca gcagttcctg ccgcacgcgg aggctttcct cgaccgggcg gcgcacctgt
     4981 gcggcatgtt ccgcgaaacc gaggaacagc aagaggtgca ggtggtcgcg agccagtacc
     5041 tgattgcgta tttgctaatc gatacggtgc ggcggtttca tcgcatgttt cctcagattc
     5101 acgtccgctt gagcgcccgc accgagcacg agatcgaaga aacgctgctc gagtcgttgg
     5161 agttctcgtt cggcgtcgcc gcgccctacg agtcgtcccc ggaactcacc taccgccact
     5221 tgttctcgat gcactggagc ctgatcgcgc cccccaggca tccgctggcc cagaagcgca
     5281 gcctgcggct agccgacgtc gtgccccatc cgctcatcat gttcgagcgc ggttccacgg
     5341 gccggcagca cgtcgccgag gcgtttcagc ggctggggct ctcgccgcac gtcgagctcg
     5401 aggcaacgac gaccgatttg atcgtgcgga tggtcgaggc gggcctgggc gtcgcgatcg
     5461 tacccttgca tccgtccgga gcggtgacgc gcggccgcaa ggttgccatt caaggcctcg
     5521 gcaagcaggt ccggcccatc gacagcggcg tcctgctccg gcgcaacgag caactgcccc
     5581 cggcggcgga aaaactgctg gactttcttt gcgcgactca atcggctgcg agcccgaatt
     5641 ccccctgatc gaagggtctt cggattttgt gaacctttgc ggtctggata gactaaagat
     5701 agcagggttt ctcctcttct atcctcgaaa gctggcctgc cacagggagg cagtcgagtg
     5761 cggtggtgct gttggctggc cgcgacgttg tccctgagcc tttcgctcac cggccgactg
     5821 gctgcccaaa ccgccccgcc cgatccggcc gctgaatcgc caaaacctat tgcggggcca
     5881 gcagttgagg cgagcccgct cgatacgttc ctgctccgcg acagcaaagg aaacctggtt
     5941 cccgtaccag ggatgccatt tgaagagttc gagcggctgc tgctcatgaa aaagggcctg
     6001 agccctcctg cggcccccgg ctttacattc gacgcactct cgctcgtcgg cgaagcgaaa
     6061 gagaagctcg ccacttttga ggtaacggcc acgatccgcg ttcgagccga aggctggatt
     6121 cgcgtaccgc tgctcctgcc ttctgcgatt ttgcgcggcc aatcgcgtca cgaggggccg
     6181 ggcgagcact ttgtggcgtt cgattccgcc gccggcggct acgtctgttg gctcaagggg
     6241 gcggatgcca ggccgcacgt cgtcacgctg gagttcgccg ccggcctgag caccgccggg
     6301 gacgacacgc gcttggcgct gtcgctgccg cgggcgaccg aatcgtcgct gcgtctgacg
     6361 gtcccggtcg cgagcatcga agctgcgcta accggcgagg ggctggcgac gacgcgctcg
     6421 gcgggcggag ctcaaagtga aatatcggtg ctgggtccgg cgggcgaact gcagctgagc
     6481 tggcacaagg gggcggcggc ggtaaagggt ccgacgcagc tcgatgccag cggcagcgag
     6541 attcaggtcc gcgtggagag ccagcaatcg atcacgagca ccgcccggct gaaagtccgc
     6601 agctacggcg gactgctgga aacgtttcag gtccgcctgc cgccgggtat ggagctagcg
     6661 cccagcgcgc tgggcccggt gggatatacg ctcacgccgc ttgccggcag cggcccagcg
     6721 cctgcgccgc gcaggaacgg ccccggcctg gtggttgagg tgcggctcga caagccgacg
     6781 ccaggcgtgg ccgacgttca actcgcggcc tccctcgttc ccgaagcgag cccagcgccg
     6841 ctgatgccgg cgcgttttga agtcctgggg gccgtccggc agcggggaac gatcgacgtc
     6901 agcgtcggcg ggcagtggca gctcgaatgg accgaagatc gctccgtccg gcgcgtcgat
     6961 ctggccgccg agacatcgcc ggcggacctc gtggcgcggt tcgactattt ccgccagcct
     7021 tgtgggctgc agctgcgcgt ggccgcgcgg ccttcgcggg tgagcgtcga gccgacccat
     7081 ctggtcttcg tcgatccgca gctggtgctc atcaagagca cgttgaaata ccggttccgc
     7141 ggttcgcggg tcaccgcgct ggccctggag cttggcgact ggaaattcgg cagcataatg
     7201 cccagcgacc tcttcgaagc gccggtgttc aaggcggacg cggcaggact cgtgcagctt
     7261 ccgtttcggc aggacaagcc gctgccggcc gagttggagc tgacgctcga ggcccaccgc
     7321 cagctcgcgc ctgcggccga gcacctcgta tttggcttgc cccgcccgct ggccgacatc
     7381 gtcgcgcccg ccacgctggt gatcgcgccg gcggacaacg tcgagctgac gccgcaggaa
     7441 gagctgctcc tggggctcac gccggacacc agcggcgtgc ggattcctga gcggcagcag
     7501 ccgccgcttg tgtatcgcga tttgggcggc ggagagccgg cgctgttcgc cacccggtat
     7561 cgaacccgca ctcggacgtc gaccgtcgtg gcccgcgcca cggcccgcat cgatcggcag
     7621 caactgcaaa tcgaacagca gctcgaatac cgcatcgcac acgagccgca gcgggccttc
     7681 acgctcctcg cgccgcgcgg gctgctgtcc agcggcaact tgcaggtcac gctgggcggc
     7741 cagccgctga gcacgagcgc agcgccggag ccgctggcgc ccgcagcgac ctccgcgcag
     7801 tccgctcccg gcgaagcggt ccagcggatc gaattcacga cgcccagcga ccagatcggc
     7861 gtctgcacga tcgtcgtccg ctacacgtct gccttgccgg cgtccgatgg tcatcagccg
     7921 ctcgacgtct ccgtgccgct agtgattcct gccgacgaag gcgaacaaca gtttcaggcg
     7981 cagacgctcg agtttctcgc gggtgaagcc tggcagatcg aaccgggcga acacacaggc
     8041 ggcgagttct cgcatcccgc tccgctgccg gccgcggccg gcggtcaggc gtttacctgg
     8101 tccagagcca caccgctgac ccac
//
LOCUS       DASRJB010000505         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_990111, whole genome shotgun sequence.
ACCESSION   DASRJB010000505 DASRJB010000000
VERSION     DASRJB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990111"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VFB80_13160"
     CDS             <1..1934
                     /locus_tag="VFB80_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34768.1"
                     /translation="KPRATLEVGSTNDPIPAKHKAVIRLHFVEGHNPQSCPAIVCCGG
                     RMDFHGAPLAHTWVKLGATAKKGATEIACEEAIAGWRVGDRIIVTATDADQHTEGLTE
                     ERVVTAIDGQTLKFEQPLAYQHAGEGEFRGEVANLSRNVVVESAAPDGVRGHTMYHRG
                     SAGSISYAEFRHLGKKDTLGRYSLHFHLCGSTMRGSYVIGASIWDSHNRWLTIHGTNY
                     LVVKDNVGYKSVGHGYFLEDGTEVYNILDRNLAVAAREGKRLPKQVLPFDQNEGAGFW
                     WANSLNSFTRNVAADCDRYGFRFEATPSSALKLVFPIMQPDGSEKETDIRTLPFVRFD
                     GNEVHSSFGLYGINLGEGVRGVGPDAKHPFVMRNTKIWNVHYGLRPQAPSLLVENITI
                     KSHYGVYHPNFDKHVYRNMTIRQTNTEPFNRGHDDDSVQHGLLTVDGLVFDGIRSGGM
                     PLIQISDDNSTGAAVSHFRGVQTVNWTDGRRQKALVNLGGGPRPQPKTAKGVPIYLHD
                     WYGPGRTAMVVSTRSPEYKASPGEYREDAPLTGDESRVREVQDVDFPSLLDPVDDLPP
                     ITVVTHVEKRAGQLLVRGSAADNGEIKRVVVNGQPAQLDAASSAWQAELPADTASITA
                     HSEDAAGNIEKLTHKVQLK"
     gene            1945..3093
                     /locus_tag="VFB80_13165"
     CDS             1945..3093
                     /locus_tag="VFB80_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34769.1"
                     /translation="MKWTSWLVILAVLGLSMPLAAQDAPRRKAAAAAKGDEPIDFARA
                     RELRRKQQSGEKLTAEEEAFLKTAVAARQAKGGQPGRPAIAEAEKTGFKPLTEMTADD
                     RYKGQDGGLYGQGRNSPPDELKKAAEVELKKIVPLDKDGKPAASGTIAFISISMSNAT
                     QEFSTFKPLADRDAAKSPRVTIVDCAQGGQAMAEWVDPSANPWQVASQRLVSAGVSPQ
                     QVQVAWVKLANKGPRGDLQEHGAKLQKDTTAVLQNAKTKFPNLRIVYLGSRIYGGYTG
                     GPLNPEPYAYEGAFVVRWLIQDQMKGSAALNYDAAKGDVKAPLLLWGAYFWGDGVTPR
                     KSDGLVWKREDFAGDGTHPSQSGREKVARMILDFCKSEELAKPWFTGK"
BASE COUNT          664 a         1101 c         1091 g          501 t
ORIGIN      
        1 ccaagccgcg ggccacgctc gaagtcgggt ccacgaacga tccgattccc gccaaacaca
       61 aggcggtcat tcggctgcat tttgtcgaag gccacaatcc gcagtcctgc ccggcgatcg
      121 tctgctgcgg cgggcggatg gactttcatg gagcgccgct ggcccacact tgggtgaagc
      181 tcggcgctac cgccaagaag ggcgccacgg aaatcgcctg tgaagaagcg atcgccggct
      241 ggcgggtcgg cgaccggatc atcgtcacgg cgaccgatgc cgatcagcac accgaggggc
      301 taactgaaga gcgcgtcgtc acggccatcg acgggcaaac gctaaagttc gagcagccgc
      361 tggcttatca gcacgcgggc gagggcgaat tccgcggcga agtggcgaat ctgagccgca
      421 atgttgtcgt ggagtcggcc gcccccgacg gcgttcgcgg gcacaccatg tatcaccgcg
      481 gctcagccgg gtcgatcagc tacgccgagt ttcgccacct gggcaaaaag gacacgctgg
      541 gccgctacag cctgcacttc cacctctgcg gcagcacgat gcggggcagc tacgtgatcg
      601 gggcctcgat ctgggacagc cacaaccgct ggttgacgat ccacggcacg aactacctcg
      661 tggtcaagga caacgtcggt tacaagagcg tcggtcacgg ctatttcctc gaggacggca
      721 ccgaggttta caacatcctc gaccgcaacc tggccgtcgc cgcccgcgaa ggcaagcggc
      781 tgcccaagca ggtgctgccg ttcgaccaga acgaaggcgc cggattctgg tgggccaaca
      841 gcctcaacag cttcacccgc aacgtcgccg ccgactgcga ccgctacgga ttccgcttcg
      901 aggcgacgcc atctagcgcc ctcaagctgg tgtttccgat catgcaaccc gatggcagcg
      961 aaaaggagac cgacattcgc acgctgccct tcgtgcggtt cgacggcaac gaagtccaca
     1021 gcagcttcgg cctgtatggc atcaacctgg gcgagggcgt ccgcggcgtc gggccggacg
     1081 ccaaacaccc attcgtgatg cggaacacca aaatctggaa cgtgcactac ggcctaaggc
     1141 cgcaggcgcc gtcgctgctg gtcgaaaaca tcacgatcaa gtcgcattac ggcgtctatc
     1201 acccgaattt cgacaagcac gtttaccgca acatgacgat tcgccagacc aacaccgagc
     1261 cgttcaaccg cggacacgac gacgacagtg tgcagcacgg cttgctgacc gtcgatggcc
     1321 tggtcttcga cggaattcgc tcgggcggga tgccgctgat ccagatttcg gacgacaaca
     1381 gcaccggcgc tgcggtgtcg cacttccgcg gcgtgcagac cgtgaattgg accgacggcc
     1441 gccggcaaaa ggcgctggtc aatctcggcg gcggtccgcg gccgcagccc aagacggcca
     1501 agggcgtgcc gatctacctg cacgactggt acgggccggg ccgcacggcc atggtcgtga
     1561 gcacgcgctc gcccgagtac aaggccagcc cgggcgagta ccgcgaggat gcgccgctga
     1621 ccggcgacga gtcgcgggtc cgcgaagtgc aggacgtcga tttcccgtcg ctgctcgacc
     1681 cggtcgatga tctgccgccg attacggtag tgacccacgt cgagaagcgc gcaggccaac
     1741 tgctcgtccg cggcagcgcc gcggacaacg gcgagatcaa gcgcgtcgtc gtcaacggcc
     1801 agccggcgca gctggatgcg gccagcagcg cgtggcaggc cgaacttccc gccgacactg
     1861 cgtcgatcac ggcccacagc gaagacgccg ccggcaacat cgagaagctg acgcacaagg
     1921 tgcaactgaa ataggagcgc aggcatgaag tggacgtcct ggctggtgat tctggcggtc
     1981 ctcggattgt caatgccgct ggcagcgcag gacgcgcccc gcagaaaggc ggcagccgct
     2041 gccaagggcg acgagccgat cgacttcgcg cgggctcgcg aactgcggcg caagcagcag
     2101 agcggcgaga agctgaccgc ggaggaagag gcctttctaa aaacggccgt cgcggcgcgt
     2161 caagccaagg gcggccagcc gggacgtccc gccatcgccg aggccgaaaa gaccggtttc
     2221 aagccgctta ccgagatgac cgcggacgat cgatacaagg gccaggacgg tggcctgtac
     2281 ggccagggcc gcaattcgcc gccggatgaa ttgaagaagg cggcggaggt cgagctgaag
     2341 aaaatcgtgc cgctcgacaa ggacggcaaa ccggcggcta gcggcacgat cgccttcatc
     2401 tcgatcagca tgtcgaacgc cactcaggag ttctcgacgt tcaagccgct ggccgatcgc
     2461 gacgcggcga agtcgccgcg cgtgacgatc gtcgattgcg ctcaaggcgg ccaggcgatg
     2521 gccgaatggg ttgatccgag tgctaatccg tggcaggtcg ccagccagcg cctggtctcc
     2581 gcgggcgtct cgccacagca agtgcaggtc gcctgggtca agctggccaa caagggcccc
     2641 cgcggcgacc tgcaggaaca cggggcgaaa ctccagaaag acacgaccgc cgtcctgcaa
     2701 aacgccaaga ccaagtttcc taacctgcgg attgtctatc tcggcagccg catctacggc
     2761 ggctacacgg gcggcccgct gaatcccgaa ccctacgcgt acgaaggcgc cttcgtcgtt
     2821 cgctggctga tccaagatca gatgaaaggc tcggcggcgc tcaactacga cgcggccaag
     2881 ggcgacgtca aagcgccgct cttgctctgg ggcgcatatt tctggggcga cggcgtcacg
     2941 ccgcgcaaga gcgacggcct ggtttggaag cgcgaggact tcgccggcga cggcacgcac
     3001 ccctcgcagt ccggccgcga aaaggtcgcc cggatgatcc tcgacttctg caagagcgaa
     3061 gagctggcaa agccctggtt caccgggaag tagtccactt aaagcggttg tgccggttct
     3121 gccgaaggta gcgaatacgc cgcgctcccg ctgcggctga atccccgctg gatcgaccca
     3181 tgctggcacg ctgctgcgcg cattggctgc tattggcagg actcacgctg ctggcggtcg
     3241 gtgctgcgcc gtttgcgttc gcccagcaac cggtggtggt ccggccggcg gcgatgtcgc
     3301 agcagttcgc tccgccgcca ttgcagccct tgcctccgcc gcagccgatg ccggaag
//
LOCUS       DASRJB010000506        10895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2105356, whole genome shotgun sequence.
ACCESSION   DASRJB010000506 DASRJB010000000
VERSION     DASRJB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105356"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2909)
                     /locus_tag="VFB80_13170"
     CDS             complement(<1..2909)
                     /locus_tag="VFB80_13170"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN34770.1"
                     /translation="MFSFQHNHRRAVFTAWNLLCAALAFASAAAAEPQLPQPAALALT
                     KYCTDCHGKEAAEARVSLARMLADDDFGRAFKDWEKAIRMLRQGKMPPKDAPQPIATE
                     RASIAAAIERTLDAYIAKHAGDPGPVVLRRLTAAEYGYTIADLTGLDLRLERNFVGDA
                     AGGEGFTNVGGAQFVQDSSLERYLEAAKAVADHAVIGAGPLAFFADPGQTGRELSAIH
                     RIKDIYRRHGFRTAAGEGATPFGLDLYPRAMFVAWRFRHRAKLGLGEVSLPQLAAGEG
                     LSVRFCEHVQSVLAAPAKSFPLSAIALRWLALAPPGDETTEAKVRTQCEEIARELREW
                     QSTLAAAAGDEEEAAVLTEGDITVRPAHSFTTAITWEEGAKSAEFELSVTSASGRPVE
                     SAVVIWRNPRLRFRRGDNRRGLFVPLVANLAPESLAELQPGQHPAGKEIGKNDFVIGG
                     EKKIVLKLVVPAGAISAQLAVDVELDRPGGGDNIVRCRIWDGSTVGKTAAAVGAASAL
                     IAHPDSPAVSAWQAGVAEFARLLPEVSHREPAPSDRDPIPPPYDNTYNLPERNHYHYA
                     IKYCRDDRFLALHMLDDASRVELDQAWTDLLTSFEYHDANLRFVAKKFKLELVGRGIA
                     DLDPTFIASQPAEPRGYIQRWYDEYRAMQQALRTAEPGHVADALKLAERAWRRPLSAT
                     EQQRLRDFYTGIRETGGLDHTQALRALIARILVAPAFLYRAEPTQQTAGVTPLSDWEL
                     ASRLSYFLWSSLPDDELRRAAAAGELRQPEQLAAQARRMLKDEKARRLAAEFFGQWLG
                     FYQFDEFQGIDAGRFPEFNEPLKAAMYDEAVSFFEHIVREDRPPAEILFGDYTFVNQL
                     LARHYGLAPADVPADRAVRIADVSGQHRGGILGMGAMLGVTSAPLRTSAVKRGDWLLR
                     RVLGTPVPPPPADAGSIPAEETLADELTVRQRLEKHRTAASCVSCHSRIDPLGFAL"
     gene            3036..4661
                     /locus_tag="VFB80_13175"
     CDS             3036..4661
                     /locus_tag="VFB80_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN34771.1"
                     /translation="MAGGIHYFWTGTIAAALWWCGSPSWASADDPPAVARPESLAAEI
                     DRRLEAHWQAQGIAPAAPADDATIYRRLMLDLTGRIPTRVEWEAWLADKTPADERYRQ
                     ALRQILDGPEFPLYWGAVLDEIVQGTAAGSPDFIDYLRRSLRERKRWDAVFRELLIGP
                     WKTDESKPAARFLEVRAKDLDRLTTDTTRVFFGVDVSCARCHDHPLVDDWKQDHYYGM
                     ASFFNRTTGGKGNLGEKFEGDVTFKGAGGTQKTAALMFLTGRVIDEPPPEEAKQSKFS
                     RREQLVAVALAEQTLLSRSLVNRLWQQFFGRGLVHPVDQMHSGNPSSVPGLLEWLAGE
                     FAADYDVQRLVAAIVSTKAYRLNSRWDSGSPLPDERHFAAMRLRLLSPRQYALSLLVA
                     SGRTDFAAAEARQARAERLLGAKGVHRAAQYLAAEELAAPLLAQLDPAQDGQSSVAEA
                     LYLSNNSAVQALLGREPGTLAARLAETADARQIAESAIGQVYCRPPAADEVERLTNWL
                     KQQEQSSQPADKHRICEQLVWALVTAAEFRFQH"
     gene            4681..5979
                     /locus_tag="VFB80_13180"
     CDS             4681..5979
                     /locus_tag="VFB80_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34772.1"
                     /translation="MRRHSTCQPAEHALHRRTFLGGLFGGAAATLTLGGVSLAGEVPA
                     ASDLRRQQKRALFIWLAGGASQFEMWDPKPGRETGGPFRTIPTAVSGYRVCELMPHLA
                     ARMNQLAVVRSLNTQISEHGQAADLMSTGWPKEPALEHPELGVVLAKELALEGSLLPD
                     YVSLFATTEGRRRPATGFLSRRHSPLLLEESLQPRNIDLPAGLTPEQHAGREELRASL
                     SSRFAEQRAGGELVQGYNEAYARVRGLMRSDELFDLAREPAAAKDRYGRTPFGQHCLL
                     ARRLLEAGVPMVKVARGFWDSHHDNFESHRELVPDFDRVLSVLLDDLHERGLLDSTLV
                     IVLSEFGRTPKINEDVGRDHYADAWSCAFAGCGIRGGTVYGQTDEDGVKVVEGEATAG
                     DVAATIFRAVGIDPARHYQVGPRPVPLAKEDAKPIKAILA"
     gene            5976..6998
                     /locus_tag="VFB80_13185"
     CDS             5976..6998
                     /locus_tag="VFB80_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34773.1"
                     /translation="MTAFDPQRATLQHKAETPGILFASCYDAASGNYYGAGTDGAVYR
                     LATRAEKPAAEKRWRHHDNYVSALGWLDGTVVSTGYDRQLVWTRAESGEKLRSSEAHD
                     GWVRDLALIPARGQIATVGDDLLVKLWDAGSGEPVKTFDGHAKQTPEGFATAIYALAA
                     SADGKLLASGDRIGEVCIWELDSGRLAARLKAPEFYTFDPQKRSRSIGGIRALLFLPD
                     GRLAIGGLGPVSNVDGFVGPARFEIWDWQNAKMLHRGQDKHHAILDHLEYLPELSLMI
                     GGGGGDAGPFLGFWNLRSEAPLHKAKPKSHIHHFVFDAASPRILAAGHAGFQIWTFTG
                     EEEKKP"
     gene            7050..7421
                     /locus_tag="VFB80_13190"
     CDS             7050..7421
                     /locus_tag="VFB80_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34774.1"
                     /translation="MKCEVCGNDYDKAFAVEMAGQRHTFDSFECAIHALAPTCGHCGC
                     HIVGHGVEQNGSIYCCVHCASQAGAHGLKDRNDTSGKAPPAGRKKTGVSKQKADALVR
                     EASEESFPASDPPAWSGGREA"
     gene            7437..7631
                     /locus_tag="VFB80_13195"
     CDS             7437..7631
                     /locus_tag="VFB80_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34775.1"
                     /translation="MTMKKSKVAASSVAAKSTVKRRSRTVARPQQPGKTIPGGEQDVR
                     RRLGNFTGAGEHARMGARRK"
     gene            complement(7650..8234)
                     /locus_tag="VFB80_13200"
     CDS             complement(7650..8234)
                     /locus_tag="VFB80_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN34776.1"
                     /translation="MSCSEFTARTVHATEEQLAFGLNQITAAPKIDGTLELIVARPQS
                     GQRVVLERGALTPEGGLSGDRWQADSWRKFPDGTPDPAVQITLMNARCIALVAGDREF
                     WPLAGDNLFVDLDLSRENLPLGTRLLIAGAILEIAKPPHNGCWKFEGRFGKAALAFVN
                     SPEGKKLRLRGVHARVVQGGSIAVGERIGVQRPA"
     gene            8333..8551
                     /locus_tag="VFB80_13205"
     CDS             8333..8551
                     /locus_tag="VFB80_13205"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZN34777.1"
                     /translation="MLEWSQCPEVERSAQRVSGAWVFKGTRVPVTALFENLEDGATVD
                     EFVEWFPGVRREQVLAVLEFASRSLVEA"
     gene            8548..8883
                     /locus_tag="VFB80_13210"
     CDS             8548..8883
                     /locus_tag="VFB80_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34778.1"
                     /translation="MSKARVRILFDQGTPAPLRHHLAAHSVSTSFELGWEQLDNGALL
                     LAAEQAGFEVFVTTDKNLRFQQNLSGRQLAILVLPFASWRRLRAHAGLIAIAIDALKP
                     SDYVEIMLP"
     gene            9002..10282
                     /locus_tag="VFB80_13215"
     CDS             9002..10282
                     /locus_tag="VFB80_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34779.1"
                     /translation="MLSLWGQKRECRRGMSRRDLLRAGALGLGGLTLAEVLKLQQAGA
                     AESKTGPVKGKSVIWIWLRGGASHIDSWDMKPAAPAEVRGEFSPIPTSVPGIEICEHL
                     PRQAAMMDRLAIVRGIKSNDLGDHTPHYILTGFADRGIRPSLGAVASYLHQQPAEVPP
                     YVSTFDYSYGESTYTGVAHQPFIPQGAGLENLRQKRDVAIGRMQDRRELLGRLDTLRR
                     DVDRSGTFDGLDAFTRQAIEMVTSEKAREAFDLNREPAAARERYGKYSESFLLARRLV
                     EAGVSVVTVKVGDWDTHEHNFRDMREQLPLLDQGFHALVSDLYDRGLQEDVAVVLWGE
                     FGRAPRISRIAGRDHWPEAGAAVLAGGGFHVGQAIGETDSHAGQSKGQPYTPANILAN
                     LYRHLGIDSAATIPGRDGRPMHILDDRRPVRELL"
     gene            complement(10283..10723)
                     /locus_tag="VFB80_13220"
     CDS             complement(10283..10723)
                     /locus_tag="VFB80_13220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN34780.1"
                     /translation="MATVFVTTRRVEFSDTDAAGIVHFVAFFRMMEQAEHDLLRSVGL
                     SVVAAGEAGTISWPRVSARCDFESAARFEEILEIEVRIARLGNKSVTYAHRISRQGQL
                     VAAGEITSVCCRLREGQPPEPIPIPAWFADKLRPFAENSSASGE"
     gene            complement(10734..>10895)
                     /gene="rpsR"
                     /locus_tag="VFB80_13225"
     CDS             complement(10734..>10895)
                     /gene="rpsR"
                     /locus_tag="VFB80_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037202082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZN34781.1"
                     /translation="VDYKDTELLKKLVNRHGKIVSRRKSGCTAVSQHAVALAIKRARH
                     MALLPYVGE"
BASE COUNT         1858 a         3590 c         3649 g         1798 t
ORIGIN      
        1 agggcgaatc cgagcggatc gatccgcgag tggcaactga cgcacgaggc cgcggtgcga
       61 tgcttttcca gccgctggcg aacggtcaat tcgtcggcga gcgtttcctc ggcgggaatc
      121 gagccggcgt cggcaggcgg cggcggaacg ggcgtgccca gcacgcggcg gagcagccag
      181 tcgccgcgct tcacggcgct cgtccgcagc ggcgccgagg tgacgcctag catcgcgccc
      241 atgcccagga tgccgccgcg gtgctgaccg ctgacatcgg cgatgcgaac ggcgcgatcg
      301 gcgggaacat cggcaggcgc cagcccgtag tgccgggcca gcaactggtt cacgaacgtg
      361 taatcgccaa acaggatttc cgccggcggc cggtcctcgc gcacgatgtg ctcgaagaac
      421 gagacggcct cgtcgtacat cgcggccttg agcggctcgt tgaattcggg aaagcggccg
      481 gcgtcgatcc cctgaaattc gtcgaactgg taaaagccga gccactggcc gaagaactcg
      541 gcggccaagc gccgggcctt ctcgtccttg agcatccgcc gcgcctgtgc cgcaagctgc
      601 tccggctggc gcagctcgcc cgcggcggcc gcgcggcgga gctcgtcgtc gggcaggctc
      661 gaccacagga aatagctcag ccggctggcc agctcccaat ccgacagcgg cgtcacgcct
      721 gccgtttgtt gcgtcggctc ggcacgataa agaaatgccg gcgcgaccag aatccgggcg
      781 atcagcgctc gcagtgcctg ggtatggtcc aggccaccag tctcacgaat gcccgtgtaa
      841 aagtcgcgca gccgctgctg ttcggttgca ctgagcggcc gccgccaggc ccgctccgcg
      901 agcttcagag cgtctgcgac gtggcccggc tccgcggtgc gcagagcctg ctgcatggcc
      961 cgatattcgt cgtaccagcg ctggatatag ccacgcggct cggccggctg gctggcgatg
     1021 aacgtcggat cgagatcggc gatgcctcgg ccaacgagct ccagcttgaa tttcttggcg
     1081 acgaaccgca ggttcgcatc gtgatactcg aacgacgtga gcaggtcggt ccaggcctgg
     1141 tcgagctcca cgcgcgacgc gtcatcgagc atgtgcaggg cgagaaagcg gtcgtcgcgg
     1201 cagtacttga tcgcgtagtg atagtggttc cgctcgggca ggttgtaggt gttgtcgtag
     1261 ggcggcggga tcggatcgcg gtcgctcggg gccggttcgc ggtgcgaaac ttccggcagc
     1321 aggcgggcga actcggcgac gccggcttgc caggcgctca ccgccgggga atcgggatga
     1381 gcgatcagtg ccgaggccgc gccgaccgca gcggccgttt tgccgacggt cgagccgtcc
     1441 caaatgcggc accgcacgat gttgtcgccg cccccggggc ggtccaactc gacatcgacg
     1501 gccagttgcg ccgaaatcgc cccggccggc acgaccagct tgagaacgat ctttttctcg
     1561 ccaccgatga cgaaatcgtt cttcccgatt tccttccccg ccggatgctg gccgggctgc
     1621 agttcggcga gcgactccgg cgcgaggttc gctaccagcg ggacgaacaa tccgcggcgg
     1681 ttatcgccgc ggcggaatcg cagccgcgga ttgcgccaga ttacgacagc actctctacg
     1741 ggccggccgc tggccgaggt gacggatagc tcgaactccg ccgacttggc gccttcctcc
     1801 caggtgatgg ccgtcgtaaa cgagtgtgcg gggcgcacgg tgatgtcgcc ctcggtcagc
     1861 acggccgctt cttcctcgtc gcccgccgcc gcggccagcg tgctctgcca ctcgcgcagc
     1921 tcgcgggcga tttcctcgca ctgggtgcgg actttcgcct cggtggtttc gtcgcctggc
     1981 ggcgctagcg ccagccagcg cagggcgatc gccgagagcg ggaacgattt ggccggcgct
     2041 gccagcacgc tctgcacgtg ctcgcagaag cggacgctca agccctcacc agcggcgagc
     2101 tgcggtagcg atacctcgcc cagacccagc ttcgcccggt gccgaaagcg ccaggcgacg
     2161 aacatcgccc gtggatacaa gtcgagcccg aacggcgtgg ctccttcacc ggccgccgtg
     2221 cggaagccgt gccggcgata gatgtccttg atgcggtgga tcgccgacag ctcgcggccc
     2281 gtctgaccgg gatcggcaaa gaacgccagc ggccccgcgc cgatcaccgc atggtcggcg
     2341 accgccttgg ctgcctccag gtatcgctcc agcgacgaat cctgcacgaa ctgtgcaccg
     2401 ccgacgttgg taaatccttc gccgccggca gcatcgccga cgaagttccg ctccaacctc
     2461 aagtccaagc ccgtcaagtc ggcaatcgtg tagccgtact cggccgccgt cagccgccgc
     2521 agcaccaccg ggcccggatc gccggcgtgc ttggcgatgt acgcgtccag cgtccgctcg
     2581 atcgccgccg cgatgctggc ccgctcggtt gcgattggct gcggcgcgtc cttgggcggc
     2641 atctttcctt gccgcaacat gcggatcgcc ttttcccaat ccttgaaagc ccggccgaag
     2701 tcgtcgtcgg ccagcatgcg ggccaggctg acccgcgctt cggccgcctc cttgccgtgg
     2761 cagtccgtgc agtacttcgt cagcgcaaga gccgccggct gtggcaattg gggctccgcg
     2821 gcggccgcgc tcgcaaaggc cagcgcagcg cagagcagat tccaagcggt gaagactgcc
     2881 cgcctgtggt tgtgctggaa agagaacatc gcacgtcccg tcgcgacctg attgtacccc
     2941 gcgggctgcc gattcacacc tatcaagctg cgttgatatt ggtgggggcc tgtgttatca
     3001 gtaacggacg gtccagttcg atgcggagac aacgtgtggc tggcggcatt cattacttct
     3061 ggaccggtac gatcgccgcc gccctgtggt ggtgcggcag cccgtcctgg gcaagcgccg
     3121 acgatccgcc ggcagtcgcc aggcctgaat cgctcgccgc ggaaatcgac cggcggctgg
     3181 aagcgcattg gcaggcccag ggaatcgcgc cggcagctcc agccgacgac gccacgatct
     3241 atcgccggct gatgctcgac ctgaccgggc gaattccgac acgcgtcgaa tgggaggcct
     3301 ggctggccga caagacgccg gcagacgaac gttatcggca ggcgctgcgg cagattttgg
     3361 acgggccgga gtttccgctc tactggggcg ccgtgctgga tgaaatcgtg caaggcacgg
     3421 cggccggcag cccggacttc atcgactatc tccgccgcag ccttcgcgag cgcaagcggt
     3481 gggacgccgt ttttcgcgag ctgcttatcg gcccgtggaa gactgacgaa tccaagccgg
     3541 cggcccgttt cctggaagtg cgagccaagg atctcgaccg gttgacgacc gatacgaccc
     3601 gcgtcttttt cggcgtcgat gtctcctgcg cccgctgcca cgaccacccg ctggtcgatg
     3661 actggaagca ggaccattac tacggcatgg cgtcgttttt caatcgcacg accggcggca
     3721 aagggaacct cggcgagaag ttcgaaggcg acgtgacctt caagggcgct ggcggcacgc
     3781 agaagacggc cgcgctgatg ttcctcaccg gccgcgtcat cgacgagccg ccgccggaag
     3841 aagccaaaca gagcaaattc agccgccgcg agcagctggt tgccgtggcc ctcgccgagc
     3901 agacgctgct cagccgctcg ctggtcaacc gtctgtggca gcagtttttc ggccgcggcc
     3961 tggtgcaccc cgtggatcaa atgcactcgg gcaacccaag ctcggtgccg ggcctgctcg
     4021 aatggctcgc cggtgagttc gcggcggact acgacgtgca gcgcctggtc gcggcgatcg
     4081 tttcgacgaa ggcgtaccga ctgaacagcc gctgggattc gggctcgccg ctgccggacg
     4141 agcggcattt cgcggccatg cgattgcggc tgctctcgcc gcggcaatat gccctgtcgc
     4201 tgctggtggc gagcggccgg accgattttg ccgccgcgga agctcggcag gcgcgggccg
     4261 aacggctgct cggcgcaaag ggtgtgcatc gcgccgcaca gtatctcgcc gccgaagaac
     4321 ttgccgcgcc gctcctggcg cagctcgatc cggcccagga cgggcaatcg agcgtggcgg
     4381 aggcgttata cctgtcgaac aattccgcgg tgcaagcgct gctgggcagg gagccgggga
     4441 cgctggccgc gcggctggcg gaaactgccg acgcgcggca aatcgccgag agtgcgattg
     4501 gccaggttta ctgccggccg cccgcggcgg acgaggtcga gcggttgaca aactggctga
     4561 aacagcaaga gcaatccagc cagccggcgg acaagcaccg catctgcgag caattggtgt
     4621 gggcgttggt gacggcggct gagtttcgct tccagcacta gagacaatca agggcgacag
     4681 atgcgcagac attcaacttg tcagccggcc gagcacgccc tgcatcgccg tacgtttttg
     4741 ggagggctat ttggcggcgc ggccgcgacg ctgacgctcg gcggcgtttc gctggccggc
     4801 gaggttccgg ccgcgagcga tctacgccgc cagcaaaagc gggccctctt catttggctc
     4861 gccggcggtg ccagccagtt cgaaatgtgg gatcccaagc cgggacgcga aacgggcggc
     4921 ccctttcgca cgattcccac agccgtgagc ggctaccgcg tctgcgagct gatgccgcac
     4981 ctcgccgcgc gcatgaacca gctggccgtc gtccgctcgc tcaacacgca aatctcagaa
     5041 catggccagg cggccgacct gatgagcacc ggctggccca aagagccggc cctggagcat
     5101 ccggagctgg gtgtcgtcct ggccaaggag ctggcgctcg aaggcagcct gttgcccgat
     5161 tatgtctcgc tgtttgccac gaccgagggc cgacggcggc ccgctacggg cttcctcagt
     5221 cggcggcaca gtccgttgct cttggaagaa tcgctccagc cgcggaatat cgacctgccg
     5281 gcgggcctga cccccgagca gcacgccggc cgcgaggagc tgcgagcttc gctcagcagc
     5341 cgctttgctg agcagcgggc cggcggcgag ctggtacagg gctacaacga ggcctacgcc
     5401 cgcgtccgcg gcctgatgcg ctccgacgag ctgttcgatc tggcgcgcga gccggcggcc
     5461 gccaaggacc gctacggccg cacgccgttc ggccagcatt gcctgttggc ccggcggctc
     5521 ttggaagccg gcgtgccgat ggtcaaggtg gcccgcggct tctgggacag ccaccacgac
     5581 aacttcgaga gtcaccgcga gctggtgccc gacttcgacc gggtgctgag cgtgctgctc
     5641 gacgacctgc acgagcgggg ccttttggat tccacgctgg tgatcgtgct cagtgaattc
     5701 ggccgcacgc ccaagatcaa cgaggacgtc ggccgcgacc attatgccga cgcctggtcg
     5761 tgcgctttcg cgggctgcgg gatccgcggc ggcaccgtgt acggtcagac ggacgaagac
     5821 ggcgtcaaag tggtcgaggg cgaggcgacg gcgggcgacg tcgcggcgac gatctttcgg
     5881 gcggtcggca tcgacccggc caggcactac caggtcggac cgcgcccggt gccgctggcc
     5941 aaagaagacg ccaagccgat caaggcgatt ctggcatgac tgccttcgac ccccagcgag
     6001 caacgctcca gcacaaggcc gaaacgccgg gcatcttgtt tgcctcctgc tacgacgcgg
     6061 cgagcggcaa ctactacggc gcggggaccg acggagccgt ctatcgcctg gcgacccgcg
     6121 ccgaaaaacc ggcagccgag aagcggtggc ggcatcacga caactatgtt tcggcgctcg
     6181 gctggctgga tggcacggtc gtttcgaccg gctacgaccg gcaactcgtc tggacgcggg
     6241 ccgaatcggg cgagaagctg cgctccagcg aggctcacga cggctgggtc cgcgatctgg
     6301 cgcttattcc agcccgcggg caaatcgcga ccgtcggcga cgacctgctg gtcaaacttt
     6361 gggatgccgg atcgggcgag cctgtcaaaa cgttcgatgg ccacgccaaa cagacgcccg
     6421 agggatttgc cacggccatc tatgctctgg ccgccagcgc ggacggcaag ctcctggcca
     6481 gcggcgatcg gatcggcgaa gtctgcatct gggaactcga ctccggccgg ctcgcggcgc
     6541 gcctcaaagc gccggaattc tacaccttcg atccgcagaa gcgctcccgc tcgatcggcg
     6601 gcattcgggc actgctcttt ctgccggacg gccggctggc aatcggcggc ctggggccgg
     6661 tcagcaacgt cgatgggttc gtggggccgg cacggttcga aatctgggac tggcaaaatg
     6721 cgaagatgct ccatcgcggc caggacaagc accatgcgat cctggaccac ctggagtatt
     6781 tgcccgagct gagcttgatg atcggcggcg gcggcggcga cgccggcccg tttctcggct
     6841 tctggaatct ccggtccgag gccccgctgc acaaggccaa gcccaagagc cacattcacc
     6901 acttcgtctt cgacgccgcc tcgccgcgga tcctcgccgc cggccatgcc ggatttcaga
     6961 tttggacctt caccggcgag gaagagaaaa agccgtaagg aagctagccg gcactccgtt
     7021 tgcacgcttt ctggcgggag gatcgtgcca tgaaatgcga agtttgcggc aatgactacg
     7081 acaaggcatt cgccgtcgag atggctggcc agcggcacac ctttgattct ttcgaatgtg
     7141 ccattcacgc gctcgcaccg acctgcggac actgcggctg ccacattgtc ggccacggag
     7201 tcgagcagaa cgggtcgatc tattgctgcg ttcattgtgc ctcgcaggcc ggcgcacacg
     7261 gcctgaagga tcggaacgac accagcggca aagcgccgcc ggctgggcgg aagaaaaccg
     7321 gcgtgagcaa acagaaggcg gatgcgctcg tgcgcgaggc atcggaggaa tcgtttccgg
     7381 ctagcgaccc gcccgcctgg tccggcggcc gggaagccta acaggagcca cgagccatga
     7441 cgatgaagaa atccaaggtg gccgcaagtt ccgtcgcggc caaatcgacg gtgaaacggc
     7501 gctcccgcac ggtcgcccgc ccgcagcagc cgggaaagac aattcccggc ggcgagcagg
     7561 acgtgcggcg gcggctgggc aacttcaccg gagctggcga gcacgcccgc atgggcgcgc
     7621 ggagaaagta gcgcgggcgc ccggcgcttt catgccggcc gctggacgcc gatccgttcg
     7681 ccgacggcaa tcgagccgcc ttgcaccacc cgggcatgca cgccgcgcag ccgcagcttt
     7741 ttgccctcgg gcgagttgac gaatgccagt gccgccttgc cgaagcggcc ctcgaatttc
     7801 cagcagccgt tgtgcggcgg cttggcgatc tcgaggatcg ccccggcgat cagcagccgc
     7861 gtgcccagcg gcaggttctc gcggctgaga tcgaggtcca caaacaggtt gtcgccggcc
     7921 agcggccaga attcgcggtc accggcgacc agcgcgatgc agcgggcgtt catcagcgtg
     7981 atttgcaccg ccgggtcggg agtaccatcg ggaaacttgc gccagctatc ggcctgccag
     8041 cggtcgccgc tcaagccgcc ttctggtgtg agcgctccgc gttccagcac gacgcgctgc
     8101 cccgactgcg gccgggctac gatgagttcc agcgtgccgt caatcttcgg cgcagcggta
     8161 atctgattca gtccgaatgc aagctgctcc tctgtcgcat gaaccgtgcg ggcggtgaat
     8221 tcactgcaac tcatcaacga tcccattaaa accaacaggc agatgacgtg gaccacgaac
     8281 ggttatcagc gtagcataaa gtggagtgat atgagagcgc ggagaagcgg cgatgcttga
     8341 gtggagtcaa tgcccggaag tcgagcggtc ggcccagcgt gtcagcggcg catgggtgtt
     8401 caagggtacg cgcgtgccgg ttacagcgct cttcgaaaac ctggaagatg gcgcgaccgt
     8461 ggatgaattc gtggaatggt tccccggcgt gcgccgcgag caagtgctgg cggtcttgga
     8521 gtttgccagc cgcagcctcg tggaagcgtg agcaaagcac gcgtgcgaat tctctttgat
     8581 caaggtacac ccgctccgct tcgccaccat ctggccgcgc attcagtttc gacgagtttc
     8641 gaactgggct gggaacagct tgacaacggg gcgctacttc tggccgcgga gcaggctgga
     8701 ttcgaagtct tcgtgacaac ggacaagaat ctccgctttc agcagaatct gtcgggacgt
     8761 cagctcgcaa tcctcgtcct tccttttgcc agttggcgac gcttgcgagc tcatgccggg
     8821 ctcatcgcca tcgcgatcga cgccttgaag ccgtctgatt acgtggaaat catgctaccc
     8881 tagaggtcat cgtattgccg aaataggagt cgcccgctga ctgcccaaag cgcataattg
     8941 cccgagctag cgcatcagca gatctgacgc ccgctgacct tggatggtag gaggcttgca
     9001 catgttgtcg ctatggggcc agaagcgcga gtgccggcgg gggatgtcgc ggcgcgattt
     9061 gcttagggcg ggcgcgctgg gattgggcgg actgacgctg gcggaagtgc tgaaactcca
     9121 gcaggctggg gcggcggaat cgaaaaccgg ccccgtcaaa ggcaagtcgg tcatctggat
     9181 ctggctccgc ggcggcgcgt cgcacatcga tagctgggac atgaagccgg ccgcgccggc
     9241 tgaagtccgc ggcgagttct cgccgatccc gaccagcgtg ccgggaatcg agatttgcga
     9301 gcacctgccg cggcaggcgg cgatgatgga ccgcctggcg atcgtccgcg ggatcaagtc
     9361 gaacgacttg ggcgatcaca cgccgcacta cattctcacg ggcttcgcgg accgcggcat
     9421 ccggccgtcg ctcggcgcgg tcgccagtta tcttcatcag caaccggcgg aggtgccgcc
     9481 ctacgtcagc acgttcgact attcgtacgg cgaatcgacc tataccggcg tggcccatca
     9541 gccgttcatt ccgcagggcg ctggcctgga gaacctgcgg cagaagcggg atgtggcgat
     9601 cggccggatg caggaccgcc gcgagttgct cggccgcctg gatacgctcc gccgcgacgt
     9661 cgatcgcagc ggcacgttcg acggcctgga cgccttcacg cggcaagcga tcgagatggt
     9721 cacgtcggaa aaggcccgcg aggccttcga tctcaatcgc gagccggcgg cagcgcgcga
     9781 gcggtacggc aagtactccg agagcttcct gctggcccgc cgactggtcg aggcgggcgt
     9841 ttcggtggtc acggtgaagg tgggcgattg ggacacgcac gagcacaact tccgcgatat
     9901 gcgcgagcag ttgccgctgc tcgatcaggg tttccacgcg ctggtcagcg atctctacga
     9961 ccgcggcctc caagaggacg tcgctgtcgt gttgtggggc gagttcggcc gcgcgccgcg
    10021 gatctcgcga atcgccggcc gcgaccactg gccggaagcg ggcgctgcgg tgctcgccgg
    10081 cggcggcttc cacgtcggcc aggccatcgg cgagaccgac tcccacgccg gtcagtcgaa
    10141 gggccagccc tacacgcccg ccaacatcct cgccaacttg tatcgccacc tcggcatcga
    10201 ctcggccgcg acgattcccg gccgcgatgg ccgacccatg cacatcctcg acgaccgcag
    10261 gccggtgcgc gagctgctgt agctactcgc ccgatgcgga cgagttctcc gcgaacggcc
    10321 gcagtttgtc cgcgaaccac gcgggaatcg gaatcggctc cggcggctgc ccctcgcgaa
    10381 gtcggcagca gaccgacgtt atctcgcccg cggccaccag ttgcccctgg cggctgatcc
    10441 ggtgagcgta ggtcacgctc ttgttgccta gccgggcgat gcgaacttcg atctccaaga
    10501 tttcctcgaa ccgcgcggcg gattcgaaat cgcaccgggc ggagactcgc ggccagctga
    10561 tcgtgcccgc ttcgccagcc gcaaccacgc tcaggcccac gcttcgcagc agatcgtgct
    10621 cggcctgctc catcatccgg aagaaggcca cgaaatgcac gatgcctgcc gcgtcggtgt
    10681 cgctgaattc gacgcggcgg gtggtgacga agaccgtggc cattccgccg gcctcactcg
    10741 cccacgtacg gcagcagcgc catgtgccgg gcacgcttga tcgccagcgc gacggcgtgc
    10801 tggctcaccg cggtgcagcc gctcttgcgg cggctaacga tcttgccgtg gcgattgacg
    10861 agcttcttga gcagctccgt gtctttgtag tccac
//
LOCUS       DASRJB010000507        11790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1267184, whole genome shotgun sequence.
ACCESSION   DASRJB010000507 DASRJB010000000
VERSION     DASRJB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267184"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VFB80_13230"
     CDS             complement(<1..1201)
                     /locus_tag="VFB80_13230"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZN34782.1"
                     /translation="MIIYGWRTLESEVGQGGFRCPSCKTQQAYRQFSHRTWFTLYFLP
                     VFPLGTENSYVQCQCCYSQFGKHALRSLPAAAESPIQAVLVEEPAHSKQPRIVQAPHA
                     RPAAPLSAGVQIPIAQPLGNVIPDAPRTSSLALASLILGLISPVLLCAFGLSLLSSLA
                     AIVTGHLALYHLKREFPRLLGQTQAITGLVLGYVLLVASIGFWSLLLGPIFRDDNRPL
                     AQAPDTNRERLLDAEMKVTGASGDDGAATGNTAAAEALARTYSEMLAKMRNAEFTPDR
                     ERKVTLSGGKFVVHCELHPGRCALLVHVPSYRDFDSDAKEELARRSWELAQELVTPEL
                     QPGDLLAVGLRGSLLYGSVMVGKVGGAPQDGFTGSERDDLIAFFPAPAAPAAEAPAKV
                     AEEPPPAA"
     gene            complement(1232..4429)
                     /locus_tag="VFB80_13235"
     CDS             complement(1232..4429)
                     /locus_tag="VFB80_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN34783.1"
                     /translation="MRSQRLLLGAFCVAVAAVQQHVAAQQAGEQPQFDPAHAAKMQEG
                     LSLFKAQVRPVLIEKCVDCHGGDEVESGFDLATRKGLLRGGAHGPAVVAGKSGDSNVV
                     RFISHKEKPFMPDGGDKLPAEQIAAIAQWIDLGAPYDKPLVANPRDPDAWTNTVVDPR
                     AREFWSLKPLAKPQPPVVKNEAWVRNPIDRFVLAKLEEKGLTPNRPAEARVLIRRAHF
                     DLIGLPVPPSLFPSIAPSGHDGEKERRRDGETEYAALLDQLLDSPHYGERWGRHWLDT
                     ARFAESHGFEQDYDRPYAYHFRDFVIEALNRDMPYDQFVRWQLAGDELAPEDPLALKA
                     TGFLGAGVFPTQITANEVERTRYDALDDMAATTGSAMLAFSVGCARCHDHKFDPIPQA
                     DYYRLLATFTTTVRSNIDVNLDPEPYKRAKAAYDREHQPLVEAVARFEREQLPARLAE
                     WEKSAAAGEVRQPAWIVLDLESAKSFAGATLTKQDDGSLLAGGTNPDQDTFTFSAKTS
                     GRNIRAIRLEALADPSLVKSGPGRAKNGNFALSDFKVRAKTTGAENAASIKLQNPRAT
                     FEQKNMPIAAAIDENETSSWAVDPQFGKDHAAVFELAEPLANDAGVTLSFQLDFKNNK
                     QHAIGRVRIAVSADLPAEVVAGGGVPESVALALQTPAEKRIGDQVGKLLAWYRHRDAE
                     WQKLDAIRAEHAKQEPQPKLVKMMVCSEGVTPIRHHTQGADFFTETFFLKRGDCDQKM
                     GQAQQSFLQVLMTAPQREKRWQVAPPEGSKLSYRRTALANWITDTEHGAGHLLARVIV
                     NRLWQHHFGRGIVATPNDFGKQGSPPTHPELLDWLAQELIRGGWRLKPLHKLIMTSAT
                     YRQDSGFRVQGSEALPLNPEPRNLNPSAVDPDNALLWRQNLRRLEAEVIRDSMLTVSG
                     TLDRRLFGPGTLDEGHTRRSIYFLVKRSKLVPMMQLFDQPEPLVSVGGRPSTTIAPQA
                     LAFLNSPHVRTYAHNFAKQLLPAYEKSPADCVRQGYLTAIGRAPDDEELATTAMFLAA
                     QEKSYAAASKPDARQLALADFCQVLFGLNEFVYVE"
     gene            complement(4519..5007)
                     /locus_tag="VFB80_13240"
     CDS             complement(4519..5007)
                     /locus_tag="VFB80_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34784.1"
                     /translation="MNTNVSLALALCTMCLVLPAAAHGEEGGGWKMPNLNPFSRKGAP
                     PTSARISDQDEGWKMPKLLPTSNSSRTAAKPKGQSAWQRMTTGTKTFMAKTADALNPF
                     DDAQDQEPVRITGSKNTVRRASAKKEQKPSPWLPSLWGHGDEERGPKTVNEFLNQERL
                     KP"
     gene            5535..6284
                     /locus_tag="VFB80_13245"
     CDS             5535..6284
                     /locus_tag="VFB80_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZN34785.1"
                     /translation="MPRKPLIGLNADYRPSRKEGPALSFLAAGYYNAIQAAGGVPVIV
                     PPMSDAADLGRVLDLLQAFVLVGGADLDPRRDGYQLHSSVRAMDPRREEFDRLLMKQI
                     AQRRLSVFGIGAGMQLLNVSQGGTLYLHLPEELPSAIPHKDPHDPEHRHALEVVFGTL
                     MERVFGDGEIRVNSMHHQAIDDVAPGFQVSARCPDGVIEAIESTQEDWWAIGTQFHPE
                     AESATALDVRIFEEFLDGVRERTRGLKLAAA"
     gene            6704..8800
                     /gene="glgX"
                     /locus_tag="VFB80_13250"
     CDS             6704..8800
                     /gene="glgX"
                     /locus_tag="VFB80_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZN34786.1"
                     /translation="MLMRHVHPTLQFTHMLPYGAILHENGVQFVVFSRSAKAMRLLLY
                     EGVDDAEPEEIIEFDPNNDRWGDIWSIFVPGAGAGQLYHFQADGPHDPERGQWFDSTA
                     RLIDPYAKALAGEFQPSPDGIVRPPKCVVVDDYFDWEGDRHLRRSVSESVIYEAHVRG
                     FTRDKTSKVKQPGTYLGLIEKIPYLQSLGVTAVELMPVHEFPTNGIYGQPLERPNYWG
                     YDPMAFFAPHRGYQVGSRPGSQVAEFKQMVKALHAAGIEVILDVVFNHTCEGNELGPV
                     LSFKGLENRVYYMLGNGGSYYMNFSGCGNTVNGNHPVVREMIFHCLRHWVHNYHVDGF
                     RFDLASILSRDRQGNLVPNPPLIEAIAEDPMLADTKIIAEAWDAAGAYQVGSFGDLRW
                     AEWNGRYRDDVRRFWRGDKAQLGAFVTRLAGSSDLYEHAGRPPYCSINLITSHDGFTL
                     NDLVCYKEKHNEPNGEGNRDGDNNNFSDNYGVEGATKRPEIENLRLRQIKNFLATLML
                     SQGVPMIVAGDECRRTQRGNNNAYCQDNETSWFNWDLVKSNESLVRFASALINFRRSQ
                     PTVRREHYLTGQPKFDGELPDVSWFSALGTAVDWHGDDSTLTALLKAVEPEHDPEGHS
                     RDVLILVNASTQPREFILPPVAKGTRWRLLLDTAAAPPYDVYPDLDGPPPPRSRRLTL
                     SYRSMCVFVADSGRGK"
     gene            complement(8801..9787)
                     /locus_tag="VFB80_13255"
     CDS             complement(8801..9787)
                     /locus_tag="VFB80_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34787.1"
                     /translation="MLKHNLRKAAANGNGVYVLGTDEAGYGPNLGPLLIGASAWRVPA
                     RQVADELYEVLAECVTADPADESGKLAIADSKRLYQPGGGLANLERGVLAAMWLLERR
                     VSRWRELWPVLDPHSLTQIDGLPWHDGFDEPLACEARELAMRLDGGCQNARVQLTGLQ
                     ATAIFPAQFNAAVGRCDNKAEVLSLATLRLAERTLFALPEGPAIVLCDKHGGRNRYAA
                     LLQEVFPDELVRVRRESGELSVYDVRAAGRAVEFRFQPKGERHLPTALASMTAKYLRE
                     LAMRPFNLFWQRQVPGLAPTAGYPGDSRRFWNEIRAAQQRLQIADDVLWRQR"
     gene            9853..11166
                     /locus_tag="VFB80_13260"
     CDS             9853..11166
                     /locus_tag="VFB80_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34788.1"
                     /translation="MPCACCLLPVLFLCTLLLAADPNSWPQFRGPAGQGHADAVGLPA
                     SWNENENIVWKCPLPGRGWSSPVMAGHEIWLTAAIESPVSEAEKAERIKGSTNSQPVS
                     VSGRLSLRALCVNRQSGRLVHNVELLVEPRPQPTHTTNSFASPTPVLESGRLYCHFGA
                     YGTACLDTATQRVVWTNRDFVINHENGPGSSPVVWGEVVIFHCDGSDEQYIVALHKGT
                     GKLAWRTARSGKMNAEPQLKKSYGTPLVVEIGGRSQLLSPAADWLYAYDPASGRELWK
                     LPYEKLGFSIAPRPVAGHGMLFMLTSFTQSELLAIDLAPQPKIAWRFARQAPQVPSPL
                     LVGDELYLLGDKGIATCLDAHTGQVHWTERLGGNYYASPLFADGRIYFFSREGQSTAI
                     APGKTFQRLGSGQLDGAICASPAAIGQALYIRTEKSLYRVEYLKQ"
     gene            11255..>11790
                     /locus_tag="VFB80_13265"
     CDS             11255..>11790
                     /locus_tag="VFB80_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34789.1"
                     /translation="MLAEVPKNAPPEKDTLQAVFDRIQNHAAGDAWKQPGWKDDAIQS
                     YLDKLIATIAFATENKDLTLPVRMGDVKPFDPTPRNAFRPEEMLLIGKNLKITNAPQR
                     SIILADGSVELMSIPQECTIVARHAIVGRSNLRSCVLVAGSYFCGQYDGMPDNPAAGS
                     LIITRGRAEMTASWGSLIA"
BASE COUNT         2008 a         3894 c         3745 g         2143 t
ORIGIN      
        1 cggcggcagg aggcggttcc tcggcaacct tcgccggcgc ttccgctgcc ggcgcagctg
       61 gcgcgggaaa gaaagcgatc aggtcgtcgc gctcgctgcc ggtgaatcca tcctgtggtg
      121 ctccgcccac cttgcccacc atgaccgatc cgtacagcag gcttccgcgc aggccgacgg
      181 cgagcaaatc tcccggttgc agttcaggcg tgacaagttc ctgcgccagc tcccaagacc
      241 gtctggccaa ctcctccttg gcgtccgagt caaaatcgcg gtacgacggc acgtggacca
      301 gcagcgcgca ccgccccggg tgcagttcgc aatgcacgac gaactttccg ccgctcagcg
      361 ttacctttcg ctcgcgatcg ggcgtgaact cggcgtttcg catcttggcc agcatctcgg
      421 aataggtcct ggcgagcgcc tcggccgccg ccgtattgcc ggttgccgca ccgtcgtcgc
      481 cgctggcgcc cgtcactttc atttccgcat cgagcaggcg ctcgcggttt gtgtcgggcg
      541 cctgtgctag cggccggttg tcgtcccgga agattggccc cagcaggagc gaccagaatc
      601 cgatcgacgc cacgagcagc acatagccca ggaccaaacc cgtgatggcc tgcgtttgac
      661 ccaacagccg aggaaactcg cgcttcaggt ggtaaagcgc cagatgcccg gtgacgatcg
      721 ccgccagcga tgaaagcagg gacaatccaa aggcacacag cagcacgggc gagatcagcc
      781 cgaggatcag actggccagg gccaatgacg atgttcgcgg cgcgtccggg atgacgtttc
      841 ccagcggctg agcaatcgga atctggacgc ccgccgacag tggagcagcc ggcctggcat
      901 gcggggcctg cacgatccgc ggttgtttcg agtgggccgg ttcctctacc aacacagctt
      961 gaatcggtga ttcagcggcc gccggcaagc tgcgcagcgc gtgtttgccg aattgtgaat
     1021 agcaacattg gcattggacg tagctgtttt cggtccctag cggaaacacc ggcaggaagt
     1081 aaagcgtgaa ccaggttcgg tggctgaact gccgatacgc ttgctgcgtt ttgcatgacg
     1141 gacagcgaaa cccgccctgg ccgacttccg actcgagcgt tcgccagccg tagatgatca
     1201 tcgccggact cctcgcagct agccgcagtg gctattccac atacacaaac tcgttcaaac
     1261 cgaacagcac ctggcagaag tcggccaggg cgagctggcg ggcgtcgggc ttgctcgcgg
     1321 cggcgtaaga tttctcctgg gccgccagga acatagcggt ggtggcgagt tcttcgtcgt
     1381 cgggcgctcg gccgatggcg gtgaggtagc cttgccgcac gcagtcggca ggggattttt
     1441 cataggcggg caagagctgc ttcgcaaaat tgtgggcgta ggtgcggacg tgcgggctgt
     1501 tgaggaaggc cagggcttgc ggggcgatcg tcgtgctcgg ccggccgccg acgctgacca
     1561 gtggctcggg ctgatcgaag agctgcatca tcggcacgag cttgctccgc ttgaccagaa
     1621 aatagatgct ccgccgcgtg tgcccttcgt cgagcgtgcc ggggccgaac agcctgcggt
     1681 cgagcgtgcc gctgacggtg agcatgctgt cgcgaatcac ctcggcttcc agccgccgca
     1741 ggttctgccg ccagagaagc gcgttgtccg gatcgaccgc ggaagggttc agatttcgcg
     1801 gttcagggtt cagggggagg gcttctgaac cctgaaccct gaaccctgaa tcctgccggt
     1861 acgtcgccga cgtcatgatc agcttgtgca ggggcttcag ccgccagccg ccgcggatca
     1921 gctcctgggc gagccagtcg agcagttccg ggtgcgtggg gggcgaaccc tgtttgccga
     1981 aatcgttggg cgtggccacg atgccgcggc cgaaatggtg ctgccagagg cggttgacga
     2041 tcacgcgggc caagaggtgc cccgcgccgt gctcggtgtc ggtgatccag ttggccagcg
     2101 ccgtgcggcg gtagctcagc ttggatcctt cgggcggcgc gacctgccag cgcttctcgc
     2161 gctgcggcgc tgtcatcagc acttgcaaaa agctctgctg ggcctggccc atcttctgat
     2221 cgcagtcgcc gcgcttgagg aagaaagttt cggtaaaaaa gtcagcgcct tgcgtgtggt
     2281 gccggatcgg cgtcacgcct tcgctgcaga ccatcatctt cacgagcttg ggctgcggct
     2341 cttgcttcgc gtgctcggcg cgaatcgcgt cgagcttctg ccactcggcg tcccggtggc
     2401 ggtaccaggc gagcaatttg cccacctgat cgccgatccg cttctcggcc ggtgtttgca
     2461 gcgccagagc gaccgattcg gggacgccgc cgcctgccac aacctctgcg ggcaaatcgg
     2521 cgctcacagc gattcgcacc cggccgatcg catgctgctt gttgttcttg aagtcgagct
     2581 ggaagctgag cgtgacgccg gcgtcgttgg ccagcggttc cgccagctcg aacacggccg
     2641 cgtggtcctt gccgaactgc ggatcgacgg cccaggagct ggtttcgttc tcgtcgatcg
     2701 ccgcggcgat gggcatgttc ttctgctcga aggtcgcccg cggattctgc agcttgatgc
     2761 tcgccgcgtt ttccgcaccg gttgtcttgg ctcgcacctt aaagtcggag agcgcgaagt
     2821 taccgttctt ggcccggccc gggccgctct tgaccagcga gggatcggcc agggcttcca
     2881 gccggatcgc gcggatgttt cgcccgctcg tcttggcact gaacgtgaat gtgtcctggt
     2941 cgggattcgt accgccagcc aagagcgagc cgtcgtcctg cttcgtcagc gtagccccag
     3001 cgaacgactt ggcgctttcc aggtcgagga cgatccacgc cggctgccgc acctcgccag
     3061 ccgcggccga cttttcccac tcggccagcc gcgccggcaa ctgctcccgt tcaaacctgg
     3121 ctacggcttc gaccagcggc tgatgttccc ggtcgtacgc cgccttggcc cgcttgtagg
     3181 gctcggggtc gagattgacg tcgatgttgc tgcgaacggt cgtcgtaaac gtcgccagca
     3241 agcggtaata gtcggcctgc gggatcgggt cgaacttgtg gtcgtggcag cgggcgcagc
     3301 cgacggagaa ggccagcatc gcgctgccgg tcgtggctgc catgtcgtcg agtgcgtcgt
     3361 accgcgttcg ttcgacttca ttggccgtga tctgcgtcgg aaaaacgccg gcccccagaa
     3421 agcccgtcgc cttcaaggcc agcgggtctt ccggcgcgag ttcgtcgcct gccaattgcc
     3481 agcgtacgaa ctggtcgtag ggcatgtccc ggttcaaagc ctcgatcacg aagtcgcgaa
     3541 agtgataggc gtacggccgg tcgtagtcct gctcgaagcc gtggctctcg gcaaagcggg
     3601 ccgtatcgag ccaatggcgg ccccagcgct cgccatagtg cgggctgtcc aggagctggt
     3661 cgagcagcgc cgcatattct gtctctccgt ctctccgtct ctccttctct ccgtcatggc
     3721 cagacggagc gatggaggga aagagcgacg gagggacggg caggccgatc aggtcaaagt
     3781 gggcgcggcg aataagcacg cgcgcctcgg ccggcctgtt tggcgtgagg cccttttcct
     3841 ccagcttcgc cagcacaaag cggtcgatcg gattgcggac ccaggcttcg ttcttcacga
     3901 ccggcggctg cggcttcgcc aggggcttca gcgaccagaa ttcgcgggcc cttggatcga
     3961 cgacggtgtt cgtccaggca tccggatcgc gcggattggc gacgagcggt ttgtcgtagg
     4021 gcgctccgag gtcgatccat tgggcgatgg cggcgatctg ctccgccggc agcttgtcgc
     4081 cgccgtcggg catgaagggt ttttctttgt ggctgatgaa ccggacgacg ttgctgtcgc
     4141 ccgacttgcc ggccaccaca gccggcccat gcgcgccacc gcgcaacagc cccttgcgcg
     4201 tcgccagatc gaatcccgac tcgacttcat cgccgccgtg gcaatcgacg cacttctcga
     4261 tcaggaccgg ccgcacctgc gccttgaaca ggctcaggcc ctcttgcatc ttggccgcgt
     4321 gcgccgggtc gaattgcggc tgctcaccgg cttgctgcgc ggccacgtgc tgctggacgg
     4381 cggcgacggc gacgcagaac gcgcccagga gaagacgttg acttcgcatc gaaagctcag
     4441 ctgcagccgc ggtgaaacac aatggcacgc cggccattct atttcagcgg caacgagatg
     4501 agcaacgcgc agccaggctt acggcttgag ccgttcctga ttcagaaact cattgaccgt
     4561 cttggggcca cgctcttcgt caccatgtcc ccatagcgaa ggcagccagg ggctgggttt
     4621 ttgctctttc ttggccgagg cgcggcggac ggtgtttttc gagcctgtga tgcgcacagg
     4681 ttcctggtcc tgggcgtcgt cgaaaggatt gagcgcgtcg gccgttttgg ccatgaaggt
     4741 cttggtgccg gtagtcattc tctgccaggc gctttgtccc ttgggcttgg ccgccgtcct
     4801 gctgctgttg cttgtaggca acagcttggg catcttccag ccctcatcct gatcgctgat
     4861 tcgcgctgaa gtcggcggcg ctcccttacg cgaaaaggga ttcagattcg gcattttcca
     4921 gccgccgccc tcctcgccgt gcgccgcggc cggcaggacc aggcacatcg tgcagagggc
     4981 cagcgccaga ctcacattcg tgttcatgct tagcctcgaa ctcgggcaga gattgtccgc
     5041 gggcttgtcg cagaaccagg gcctggcggc aagaggaatc tgcactgcgc ctcggcgcac
     5101 agcagctagc aggcggctgc atgcacagga tgtggtgcta tcacttcacc aaaacacaaa
     5161 atatggttcg aggcttgcac caatgttgat catgtttatc ttttggcccc tgcagccgcc
     5221 gcattgttcg cggcggacga tcagaggatt cgcttaggat agggagatcg cttcagcgca
     5281 ggttcgggat gaacgtcgcg ctgaaggagt caggattcgg gcccagcgag gcctcgtcag
     5341 ggatgaccgc ggcccctctc ctccaccggc cgcggaataa cccagccgag ccgcgagccg
     5401 cagccttggc tgcggcgtgg ccccgttctg cgccttcacg caacgccttc caacttgggc
     5461 tgtgctcggc atggtcgccg ggctcgttcc cgccagggat ttcgggttgt tcctccacag
     5521 gaaaggaatt tgcgatgcct cggaagccac tcattggatt gaacgccgat tatcgcccct
     5581 cgcgcaagga gggcccggct ttgagcttcc ttgccgccgg ctattacaac gcgatccagg
     5641 ccgccggtgg agttcccgtg atcgttccgc ccatgtcgga cgcggccgac ctcggccgag
     5701 tgctcgatct gctgcaggcg ttcgtcctcg tcggcggggc cgatctcgat ccgcgccgcg
     5761 atggctatca gctgcatagc agcgtacgcg cgatggatcc ccgccgcgag gaatttgacc
     5821 gcctgctcat gaagcaaatc gcgcagcgcc gcttgagcgt attcggcatc ggtgcgggca
     5881 tgcagctgct caacgtctcg cagggaggca cgctgtacct gcacctgccc gaggaattgc
     5941 cgtccgcgat tccgcacaag gacccgcatg acccggagca tcgccatgct ctggaggtcg
     6001 ttttcggcac gctgatggag cgggtcttcg gcgacggcga gatccgcgtc aacagcatgc
     6061 accaccaggc gatcgacgac gtcgcccccg gcttccaggt ctcggcccgc tgccccgacg
     6121 gcgtgatcga agccatcgag agcacgcagg aggactggtg ggccatcggg acgcagttcc
     6181 accccgaagc cgagtcggcc accgcgctcg acgtccggat cttcgaggaa ttcctcgacg
     6241 gcgtccgcga acgcacccgc ggcctgaagc tcgccgcggc gtaacgtggg gcgggcgtct
     6301 cgcctgcccg tcaactggtt cccaagctcc gcttcccccg gccgcgaagc tgagcttcgc
     6361 cagacgtgcg tgcccaagcg gagcttgggc acgagggtct ctgtgcgcga aaacctcact
     6421 actaccgtca gcgtcaaccc gcgccctaca ctctccgcgc tacgctttgc gccgccagcg
     6481 ccgcttcgca cggcggcggc aagcgtccat cgcataaact ctcggtgacc tgtgcgacag
     6541 gcgtcggggg cgcgtttttc tgagctatat tgcgctgccc gcccgcctcg accgctgccg
     6601 ggtgttacgg tttttagcca gttcaaagct ccgcgttcgg cgctgcttgc cgcagcggag
     6661 ttgggaaggt cgaactggtc tgccgcgctg tcgaggcccg cccatgctca tgcgtcacgt
     6721 ccacccgacc ctccagttca cccacatgct gccgtacggc gctattctcc acgagaatgg
     6781 cgtccagttc gtcgtcttca gccgcagtgc gaaggcgatg cggctgctgc tttatgaagg
     6841 cgtggacgat gcggagccgg aagagattat cgagtttgac cccaacaacg accgctgggg
     6901 cgacatctgg agcatcttcg tgccgggggc cggcgccggg caactctatc actttcaggc
     6961 cgacggtccg cacgatcccg agcgcggcca gtggttcgat tcgacggccc ggctgatcga
     7021 tccgtatgcc aaggccctgg ccggcgagtt ccagccgtcg cccgacggca tcgtccgccc
     7081 gccgaagtgc gtcgtcgtgg acgactattt cgattgggaa ggcgaccggc acctccgccg
     7141 cagcgtcagc gagtcggtaa tctacgaagc acacgtccgc ggtttcacgc gcgacaaaac
     7201 cagcaaggtc aagcagccgg gaacctacct ggggctgatc gagaagattc cctatctcca
     7261 gtcgctgggt gtgacggcgg tagagctgat gccggtgcac gagtttccca ccaacggcat
     7321 ctatggccag ccgctggagc ggccgaacta ctggggctac gacccgatgg cgttcttcgc
     7381 cccgcaccgc ggctaccagg tcggcagccg gcccggctcg caggtcgccg agttcaagca
     7441 aatggtcaag gccctgcacg cggccggcat cgaggtgatc ctcgacgtcg tcttcaatca
     7501 cacctgcgag ggcaacgagc tcggtcccgt tctctcgttc aagggactgg agaatcgcgt
     7561 ctattacatg ctcggcaacg gcggcagcta ctacatgaat ttcagcggct gcggcaacac
     7621 cgtgaacggc aaccaccccg tcgtccgcga gatgatcttc cattgcctgc ggcattgggt
     7681 gcacaactat cacgtcgatg gcttccgctt cgatctggcg tcgatcctca gtcgcgaccg
     7741 gcagggcaac ctggtgccca acccgccctt gatcgaggcg atcgccgaag acccgatgct
     7801 ggccgatacc aagatcatcg ccgaagcctg ggatgccgcc ggcgcctacc aggtcggcag
     7861 cttcggcgac ctgcggtggg ccgagtggaa cggccgctac cgcgacgatg tgcggcggtt
     7921 ctggcgcggc gacaaggccc agcttggcgc gttcgtgacc cggctggccg gctccagcga
     7981 cctttatgag cacgccggcc gcccgccata ctgctcgatc aacctgataa cctcgcacga
     8041 cggcttcacg ctgaacgatc tcgtctgcta caaggagaag cacaacgagc ccaacggcga
     8101 aggcaaccgc gacggcgaca acaacaactt cagcgacaac tacggcgtcg aaggggcgac
     8161 caagcggccg gagatcgaga acctgcggct gcggcagatc aagaacttcc tggccacgct
     8221 gatgctcagt cagggcgtgc cgatgatcgt tgccggcgac gaatgccgcc gcacgcagcg
     8281 cggcaataat aacgcctatt gccaggacaa cgagacctcg tggttcaatt gggaccttgt
     8341 gaagagcaac gagtcgctgg tgcgttttgc ctcggccctg attaatttcc gccgctcgca
     8401 gccgacggtc cgccgcgaac actacctcac cggtcagccg aagttcgacg gcgaattgcc
     8461 cgacgtgagc tggttcagcg ccctgggcac cgcggtcgat tggcacggcg acgactcgac
     8521 gctgaccgcc ctgctcaaag cggtcgagcc cgagcacgac cccgaagggc actcgcgcga
     8581 cgtgctgatc ctggtcaacg ccagcacgca gccgcgggaa ttcatcctgc cgccggtggc
     8641 caaaggcacg cggtggcggc tcttgctcga tacggccgcc gcgccgccat acgacgtcta
     8701 tcccgatctc gacggcccgc cgccgccgcg ctctcgacgg ctgacgctct cgtaccgctc
     8761 gatgtgcgtc ttcgtggccg acagcggccg cgggaaatag ctatcgctgt cgccagagca
     8821 cgtcgtcggc aatttgcagc ctttgttggg ccgcgcggat ctcgttccaa aaccgccgcg
     8881 aatcgcccgg atagccggcg gtcggcgcga ggcccggcac ctgccgctgc cagaacaggt
     8941 tgaacggccg catcgccagc tcgcgcaggt acttggccgt catcgaggcc agggccgtgg
     9001 gcagatgccg ctcccccttg ggttgaaagc ggaactcgac cgcgcggccc gctgcgcgga
     9061 cgtcgtagac gcttaactcg ccgctctcgc ggcggacgcg cactagctcg tcgggaaaaa
     9121 cctcctggag cagggccgcg tagcgattgc ggccgccgtg cttgtcacag agcacgatcg
     9181 ccggcccctc cggcagcgca aagagcgtcc gctcggccag ccgcagcgtc gccagcgaaa
     9241 gcacttccgc cttgttgtcg cagcggccga cggcggcgtt gaactgtgcg ggaaagatgg
     9301 ctgtcgcttg caacccagtc aattgcacgc gcgcgttttg acagccgccg tccagccgca
     9361 ttgccaactc tctggcttcg caggctagcg gctcatcgaa accgtcgtgc cacggcaaac
     9421 cgtcgatctg tgtcaaggag tggggatcga gcaccggcca gagctcgcgc caccggctga
     9481 cgcggcgctc gagcagccac atcgccgcga gcacgccccg ctccaggttc gccaggccgc
     9541 cgcccggctg atagagccgc ttggaatcgg cgatggccag cttgccgctt tcgtcggccg
     9601 gatcggcggt cacgcattcg gcaagcactt catacagttc atccgccacc tgccgcgcgg
     9661 gcacccgcca cgccgaagcg ccgatcaaca gcggccccaa attcgggcca tagccggctt
     9721 cgtccgtgcc aagcacataa acgccgttcc cgttcgccgc ggccttgcgt aagttatgct
     9781 tcagcataac gtgcttccag aagcgtgccg tccttgagtt gacgtagcat aacgaactcg
     9841 ccgagtcctg ccatgccctg tgcttgctgc ttgctgccgg tcctgtttct ctgcactctc
     9901 ttgctcgccg ccgatcccaa cagctggccg cagtttcgcg gccccgctgg ccaaggccac
     9961 gccgacgccg ttggattacc tgcatcgtgg aacgagaacg agaatatcgt ctggaagtgc
    10021 ccgctccccg gccgcggctg gtcgagcccg gtgatggccg gccacgagat ctggctcaca
    10081 gcagcgatcg aatcgccggt cagcgaggcg gagaaagccg agcggatcaa agggtcgacc
    10141 aacagccagc cggtgagcgt ctcgggccgg ctcagcctgc gggcgctgtg cgtgaaccgg
    10201 cagagcggcc ggctcgtgca caacgtcgag ctgctcgtcg agccccgccc gcagccgacg
    10261 cacacgacca actcattcgc cagcccgacg ccggtgctcg aaagcggccg gctctattgc
    10321 cactttggag cgtacggcac cgcctgcctc gacacggcga cccagcgcgt ggtttggacc
    10381 aatcgcgact ttgtcatcaa ccacgagaac ggccccggca gctcgccggt cgtgtggggc
    10441 gaagtggtca tctttcattg cgatggcagc gacgagcagt acatcgtggc cctccacaag
    10501 ggaacgggca agctcgcctg gcgaaccgcc cgcagcggca agatgaacgc ggagccgcag
    10561 ctcaaaaagt cgtacggcac gccgctggtc gttgaaatcg gcggtcggtc gcagctgctc
    10621 tcgcccgcgg cggactggct ctatgcctac gacccagcca gcggccgcga gctgtggaag
    10681 ctgccctatg aaaagctcgg cttctcgatc gctccacggc cggtggcggg ccacggcatg
    10741 ctttttatgc tcaccagctt cacgcagtcg gagctgctgg cgatcgatct cgccccgcag
    10801 ccgaagatcg cctggcggtt tgcccggcag gccccgcagg tgccctcgcc gctcctggtc
    10861 ggcgacgaac tctacctgct cggcgacaag ggcatcgcga cgtgcctcga cgcccacacc
    10921 ggtcaggtcc attggaccga gcggctaggc ggcaactact acgcgtcgcc gctgtttgcc
    10981 gacggccgta tctatttctt cagccgcgag ggccaatcga cggccatcgc ccccggcaag
    11041 acatttcagc ggctcggctc cggccagctc gacggcgcga tctgcgcctc gcccgccgcc
    11101 atcggccagg ccctctacat ccgcaccgag aagtccttgt atcgcgtgga gtatttgaag
    11161 cagtaagcta gtggcggagg aacgccctat gttccgcaca ctcacggtcg cattcgcctg
    11221 ggcaagttgc gcgtggctag cctgttcctt tcctgtgctc gccgaagtgc cgaagaacgc
    11281 gccacctgaa aaggacacgc tccaagcggt gttcgaccgg atacaaaacc acgcggcggg
    11341 cgacgcctgg aaacagccgg ggtggaagga cgacgcgatt cagtcgtatc tcgacaagct
    11401 catcgcgacg atcgcctttg ccacggagaa caaggacttg acgctgccgg tgcggatggg
    11461 cgacgtgaag cccttcgatc ccacgccgcg gaatgcattc cgaccggagg agatgttgct
    11521 catcggcaaa aatttgaaga tcactaacgc gccgcaacgg tccatcatcc tggccgacgg
    11581 cagcgtggag cttatgtcga ttccgcagga atgcaccatt gtggcccgtc acgccatcgt
    11641 gggcagatcg aacctgcgca gttgcgtgct cgtcgcggga agctacttct gtgggcagta
    11701 tgacggcatg cctgacaacc cagccgccgg aagcctgatc attacgagag gccgggccga
    11761 gatgacggca tcgtggggtt ccctgatcgc
//
LOCUS       DASRJB010000508         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2105727, whole genome shotgun sequence.
ACCESSION   DASRJB010000508 DASRJB010000000
VERSION     DASRJB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105727"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFB80_13270"
     CDS             <1..645
                     /locus_tag="VFB80_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34790.1"
                     /translation="DQSQQLDADRSAVLAERRAWDEQQARQRQALDEARRAADAEIAE
                     RRQRLEDRQEWLERQKATLEQVRGEIVALHRQSLEMRLLAEQLWSQIAGRLPSVEVTH
                     SIAQLRMKLADQYKLEEANLASRREELLELGERITQRHRELTQLRSGLREWAAARQSD
                     IAGQAAALVRREAELDQQQERLRQAEQDWSAARRGYEQQIRDLTTQLRALPAAA"
     gene            684..1280
                     /locus_tag="VFB80_13275"
     CDS             684..1280
                     /locus_tag="VFB80_13275"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HZN34791.1"
                     /translation="MTPSVILLAGPNGAGKTTASKVVLPRLAQVTEFVNADVLAQGLS
                     GFAPEQHAIEAGRVMLARLRELAAQRANFAFEATLASRSFAVWIAELIHRGYDFQLFY
                     FWVPSAEFAIKRVANRVKMGGHSIDPETIRRRYERGLRNFFELYQPLASSWYFYDNTV
                     DSGEVPIASGRGRIVEHVDDPAIWNMLISKYAPKHQAP"
     gene            1324..1479
                     /locus_tag="VFB80_13280"
     CDS             1324..1479
                     /locus_tag="VFB80_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34792.1"
                     /translation="MRQAGREARLLHKQLGVPLVIWRDGQIVEIPPEEIVVDLFPRKP
                     EAQAKAE"
     gene            complement(1497..2837)
                     /locus_tag="VFB80_13285"
     CDS             complement(1497..2837)
                     /locus_tag="VFB80_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN34793.1"
                     /translation="MRTIVILAIALLAAGGIIAASYQPATNYWQMRSMPKWRTAEVSE
                     GEIISVVNSTGTLKPVLQVSVGSFVSGPIDAEYELKDVDGKPLLDKLGQPLHLAEFNQ
                     EVRKGDQMAKIDERIYKTAVDRDHASLLTRQADLERVEAQLELANRDKNRAMDLQKKD
                     AEFISKAEIDKFNYGWMSLLAQKKLAEASVQQAQSQLEQSQANLGYTEIKAPVDGIVI
                     NRKIDPGQTLAAQFQTPELFVVAPNMREKMHVHASVDEADIGLIHGAQQKQLPVTFTV
                     DGYPDDLFTGEIEEIRLSSTTTQNVVTYPVLVAAKNPDLKLLPGMTASISFEVDRRHN
                     VVKVPNSALRFFPLPRHVRKEDQPLLEGKAQQQQEEEVGQLAEATLSASERSEARRNR
                     SRRHVWVLDGFKLKAIEVRTGLSDSQSTELISGDLKPGMKLIIGIDTTTTGWGG"
     gene            2982..>3365
                     /locus_tag="VFB80_13290"
     CDS             2982..>3365
                     /locus_tag="VFB80_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34794.1"
                     /translation="MRQICPVLAVLAALAVGPASAQEAADKSVADLVKQLDAESFADR
                     QAAGEELVKRGKGAIEALVLATRSESAETVTRSLDILRRQSESKEAEVKVAAVAALQK
                     LASGEGLVARRAGELLKPKPVTSAAT"
BASE COUNT          580 a         1075 c         1114 g          596 t
ORIGIN      
        1 gaccagtcgc agcagctcga cgccgaccgc tcggccgtcc tggccgagcg ccgcgcctgg
       61 gacgagcagc aggcgcggca gcggcaagcg ctggacgaag cccgccgggc ggccgacgcg
      121 gaaatcgccg agcggcgtca gcggctggaa gaccgccagg agtggctcga gcggcagaag
      181 gccacgctcg agcaggtccg tggcgagatc gtggcgctgc accggcagtc gctcgagatg
      241 cggctcttgg ccgagcagct ctggtcgcag atcgccggtc gcctgccgtc cgtggaagtg
      301 acgcactcga tcgcgcagct gcggatgaag ctggccgacc aatacaagct ggaagaagcc
      361 aatctggcgt cgcggcgcga ggagctgctg gagctgggcg agcggatcac ccagcggcat
      421 cgcgaactga cgcagctccg cagcggcctg cgcgagtggg ccgccgcccg gcagtccgac
      481 atcgccggcc aggccgccgc gctggtgcgg cgcgaggccg aattggacca gcagcaagag
      541 cgcctccggc aggccgagca ggactggtcc gccgcccgcc gcggctacga gcagcaaatc
      601 cgcgacctca ctacgcagct ccgcgccttg ccggcggctg cgtaggcgta gcgggcagcg
      661 gtcggctgcg atagcgtgac ggcatgaccc ccagcgtgat cttgctcgcc ggtcccaatg
      721 gagccgggaa aacgacggcc tccaaggtcg ttttgccacg cctggcccag gtgacagagt
      781 tcgtgaatgc cgacgtgctg gcgcagggac tgtcgggatt tgctccggaa caacacgcca
      841 tcgaagccgg ccgcgtcatg ctggcgcgat tgcgcgagct cgccgcgcag cgtgccaact
      901 ttgcgtttga ggccaccttg gcgtcacgct cattcgcggt ctggatcgcc gagctgattc
      961 accgaggtta cgattttcag ttgttctact tctgggttcc gtcggctgaa ttcgcgatca
     1021 agcgagtggc gaatcgcgta aaaatgggag ggcactcaat cgaccccgag acgattcgca
     1081 ggcgctacga gcgaggtctg cgcaatttct tcgagctgta tcagccgctc gcgtcgagct
     1141 ggtacttcta cgacaacact gtggattccg gagaagtccc gattgcgagc ggacgaggta
     1201 gaatagtgga acatgtagat gatccagcta tctggaatat gctgatttcg aagtatgctc
     1261 caaaacatca agcgccttag tgtctcggaa cttctggccg acagagaccg cgtcaccgaa
     1321 gccgtgcgcc aagcgggacg cgaggcccgg ctgctgcaca aacaactcgg cgtgccgcta
     1381 gtcatctggc gcgacgggca gatcgtggaa attccgcccg aagagatcgt cgtcgatctg
     1441 ttcccgcgca aaccggaagc gcaagcgaaa gccgaataaa ttgaggcgtc cgacgcctac
     1501 ccgccccagc cggtcgtcgt ggtgtcgatc ccgatgatca gtttcatgcc gggcttgagg
     1561 tcgccggaga tcagctcggt cgattggctg tcggagaggc cggtccgcac ctcgatggcc
     1621 ttcagtttga agccgtccag cacccagacg tgccgccgcg agcgattccg ccgcgcctca
     1681 gaccgctcgc tggccgagag cgtcgcctcg gcgagctgcc cgacttcttc ctcctgctgc
     1741 tgctgagcct tgccctccag gagcggctga tcctccttgc gcacgtgccg cggcagcgga
     1801 aagaaccgca aggccgagtt cggcaccttg acgacgttgt gccggcgatc gacctcgaac
     1861 gaaatgctgg cggtcatccc cggcagcagc ttcaggtccg ggttcttcgc ggcgacgagg
     1921 accggatagg tcacgacatt ttgcgtcgtc gtgctgctga ggcggatctc ttcgatctcg
     1981 cccgtgaaca gatcgtcggg ataaccatcg acggtgaagg tgaccggcag ctgcttctgc
     2041 tgagcgccgt ggatcagccc aatgtcggcc tcatcgacgg aggcgtgcac gtgcatcttc
     2101 tcccgcatgt tcggggcgac gacgaacagt tccggcgtct gaaactgggc ggccagcgtc
     2161 tggcccggat cgatcttgcg gttgatcacg atgccatcga cgggggcctt gatctcggtg
     2221 tagcccaggt tggcctgcga ctgctcgagc tgcgactggg cctgctggac cgacgcctcg
     2281 gccagcttct tctgggccaa gagcgacatc cagccatagt tgaacttgtc gatctcggcc
     2341 ttggagatga actcggcgtc cttcttctgg aggtccatgg cccgattctt gtctcgattg
     2401 gcgagctcca gctgggcctc gacccgctcc aggtcggcct gccgcgtgag caggctggca
     2461 tgatcgcgat cgaccgccgt cttgtagatc cgctcgtcga tcttcgccat ctggtcccct
     2521 ttgcggacct cctggttgaa ttcggccagg tgcagcggct ggcccagctt gtcgagcagc
     2581 ggcttgccgt caacgtcctt cagctcgtac tcggcgtcga tcggccccga gacgaacgag
     2641 ccgaccgaga cttgcagcac cggcttgagc gtgcccgtcg agttgaccac cgagatgatc
     2701 tcgccctccg acacctcggc ggtccgccac ttgggcatgc tccgcatttg ccagtagttc
     2761 gtcgccggct gatagctcgc ggcaatgatg ccgccggctg ccagcagggc tatcgccagg
     2821 atcacgatgg ttcgcatagg ggtaattcgg tccgcggccg gcggtcttaa agagctatca
     2881 gtttaatcgg catcgccctt tcccggcatt ggagtccgcc cgcggctgcc gctaccatgg
     2941 agggcttggg acgatcgccc cgttcaccag gagtcgatgc catgcggcaa atctgcccgg
     3001 ttttggccgt tctcgcagca ctcgcagtcg ggcccgcatc tgcccaggag gctgcggaca
     3061 agtcggtggc cgacctggtc aaacagttgg acgcggagag ctttgccgat cggcaagcgg
     3121 ccggcgaaga gctggtcaag cgcggcaagg gggcaattga ggcgctcgtc ctggccacgc
     3181 gatcggagag cgccgagacg gtaacccgct cgctcgatat cctgcgccgg cagtcggagt
     3241 cgaaggaggc cgaggtcaag gtagcggcgg tcgcagcgct acaaaagctg gccagcggcg
     3301 aggggctggt ggcccgccgc gctggggagt tgctcaagcc caagcccgtc acctcagcgg
     3361 ccacg
//
LOCUS       DASRJB010000509        10143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3523535, whole genome shotgun sequence.
ACCESSION   DASRJB010000509 DASRJB010000000
VERSION     DASRJB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3523535"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFB80_13295"
     CDS             <1..527
                     /locus_tag="VFB80_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN34795.1"
                     /translation="EQQGVWSSLLAGDFDPAKLGQTKFKSPDLIGGPGQWVNGQPLTG
                     QQLRGRVVVVHYFAFGCINCIHNYPTYRKWQDELAGKNVQLVGIHTPETAGEHNALTL
                     KSKLKAERLDFPVLVDNEKANWTAWGNTMWPAVYVLDQEGYLRSFWAGELHWQGRDGE
                     AVLRKQIDSLLDGK"
     gene            complement(643..1341)
                     /locus_tag="VFB80_13300"
     CDS             complement(643..1341)
                     /locus_tag="VFB80_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34796.1"
                     /translation="MFASLCRCRSALTASLITCLLTGTARADNRDLIEVYKSRAEVIS
                     ADAQNKQANAAIITAAATFTRAQAESAKMGQETREKKLQNDLLGAKVFYDKRALYHTY
                     KDTHQTPRSTADQYTERARSAAPERLSSYQLWIKPGYVRWPSLLLAIDYSEPRSQIDY
                     LLVRRTPADSGAGSQNCVDLQQQIDTLRLALQGRMRTYKPADYLAARKFLDSLAYEVQ
                     FPADFGGATAQISK"
     gene            1620..2264
                     /locus_tag="VFB80_13305"
     CDS             1620..2264
                     /locus_tag="VFB80_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN34797.1"
                     /translation="MMKYVVELIGTFFLVLTIGNCVLAPNDAVGNMAPLAIGSALMVM
                     IFAGGHVSGAHYNPAVTLAVFLRGKCPAADVLPYIAAQVVGAAAAAAIVLYLKGLSGI
                     PPATPIPADNILRAIVAEFLYTFALCYVVLNVATAKGTSGNSFYGLAIGFTVLTGAYS
                     VGAISGGAFNPAVAVGLIVLGKVAATSIWIFLVANFAAGAAAAFVFKTINPTDK"
     gene            complement(2298..3323)
                     /locus_tag="VFB80_13310"
     CDS             complement(2298..3323)
                     /locus_tag="VFB80_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZN34798.1"
                     /translation="MSRNEQLVRQHKLLQILERTRIGWTTLELRDGMAQELGLPSLHL
                     RTVKRDLEALLASGFDIEGQYLRRGKVWRLGRKSRKAHPIQASATELIALSIGRDLLY
                     PLAGTPFWQALESFWDKLKDGLTPSVWKHYEAYRQVLYVRGMPAKSYQQQHGILSTID
                     RAIRERRVLAIEYQPTGREPQQREIEPYAVVFYQSSLYIVAAARELPADHADRIRHLK
                     LDRFLRATAQSVFFHRPEDFDLEEHLGQGVGIFSGGKARDFKIRISAKGARWVVEDPW
                     HANQRIETLSGGDLVLTVPAHHDLEIVPRVLALGSEAEVLAPASCRAAVAVIVRQLAE
                     RYAQAEP"
     gene            complement(3401..3988)
                     /gene="fae"
                     /locus_tag="VFB80_13315"
     CDS             complement(3401..3988)
                     /gene="fae"
                     /locus_tag="VFB80_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HZN34799.1"
                     /translation="MAKKKQKDKDGKKRKRIVLRAGEALVEAEAAWSAAEPEVVIGEL
                     DGPVGYAIASLIGDQAKGHSKVFAILNSDVMVRPVTVMVSKVTVKSSKYTNILMGTVQ
                     AAIAHGVLDAVRAGDIPRRKVDDLGIIVSVWLDPGILDAENVDFNILFQTNREATAKA
                     IHKALHGEPSIDWLLENQDQVPHYFHQLALEGKFE"
     gene            4089..4742
                     /locus_tag="VFB80_13320"
     CDS             4089..4742
                     /locus_tag="VFB80_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN34800.1"
                     /translation="MDERDIIQQTAEHVRALLSGEGTGHDWHHIERVRRAALAIGQGE
                     GADLFVVELAALLHDIADWKFAGGDHEAGPRQSRAWLQSLGVDARAIDHVCEIVAGLS
                     FQGAGVKAAVLTLEGQCVQDADRLDALGAIGIARAFAYGGHKGQPLYEPDAPPTPHDS
                     FEAYQRGASSTINHFYEKLLLLRDRMNTAAGRRLAAERHRFLEAYLEQFYAEWNGQR"
     gene            4961..5329
                     /locus_tag="VFB80_13325"
     CDS             4961..5329
                     /locus_tag="VFB80_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34801.1"
                     /translation="MRFTIADLLVATSIAGVGVGALVAADRWFNVPAWVHLAAAPVVV
                     LSTVGWLLGQFRGALVGAVIGFAIGLFATIAAAVLHGGSSRGEDPVLPSVFHQARIPR
                     ASSHARCCYLTASGSYDSDR"
     gene            5582..6460
                     /gene="murB"
                     /locus_tag="VFB80_13330"
     CDS             5582..6460
                     /gene="murB"
                     /locus_tag="VFB80_13330"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZN34802.1"
                     /translation="MAIPAGFEHITRENEPLAPYTWFRLGGAAQYFAEPTSVEELTGL
                     VRRCHEMGLGVRVLGGGSNLLVRDEGIPGLVVSLGAAAFGRIQVAGRKLTAGGGAKLG
                     HVISTAVREGLAGLESLVGIPGTLGGALHTNAGTLGGDIGQFTTTATAMTGKGETVTR
                     QKGEFRFGYRASSLDELVILEATLELEPSDSRHLTKQMQQAWIIKRAEQPLFDQKTGQ
                     IFKSPGGVSAASLIEDARLDTAHVGDAAISDRNANFIVVGASATSRDVIELIEVVRKG
                     VAERVGVELELAVEVW"
     gene            6573..7442
                     /locus_tag="VFB80_13335"
     CDS             6573..7442
                     /locus_tag="VFB80_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34803.1"
                     /translation="MGTNKPKTPRPSPVAAQLVAALLKPQNRGLVLTAAIVVAAVCGA
                     VYAWQRFGAAALNSADYAVTPAQIAVTPQPAWIHADVKAEALRSASMSRLNLRDRDLV
                     EKVARAFALHPWVAKVVRVEKRNPPQVSVELEYRRPVAAVEVSGGGEAGLVFIDEQGV
                     LLPSADFAPGQAKDYLRIAGGRETTASVYGSPWGSQRIAGAARLAALWGKRWQPLGLF
                     RIDAEQPPGQELNYELKTRRGVRVIWGPIAGSSREPTAEQKIAALEQFISDKGPLERE
                     GGPAVLDLCELAR"
     gene            complement(7530..7742)
                     /locus_tag="VFB80_13340"
     CDS             complement(7530..7742)
                     /locus_tag="VFB80_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HZN34804.1"
                     /translation="MLVLSRKVGERVLIGDRIAITIVRINGNAVRLGIEAPADMVVVR
                     QELAERTAEGEATATEATSPLPLGEG"
     gene            8103..9506
                     /locus_tag="VFB80_13345"
     CDS             8103..9506
                     /locus_tag="VFB80_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34805.1"
                     /translation="MASSTGRYATAQDQPASKSPNERLNVACVGVKGRGGSHISAWAA
                     KPDTMVTYVVDVDSKIGNGRVDEIEKRQGVRPKLVDDIRDVLEDKSVDIISVATPNHW
                     HSLMAIWAMQAGKDVYVEKPVSHNVSEGRRAVETARKYKRICQTGTQCRSNPGMRQLI
                     EYVQSGKIGAVKVARGLCYKPRGSIGAKGTYEVPASVNYDLWCGPARMLPVTRPQFHY
                     DWHWIWEYGNGDLGNQGIHQMDIARWGLGVNELCKSTFSYGGRLGYEDAGETANTQVC
                     VHDFGDKSLVFEVRGLRTDRYKGAGVGVIFECTEGYAVSPSYNGGTVFDKEGKEIARF
                     SEGEDKYHFANFLAAVRTRKLTDLNADIEEGHLSSALCHLGNISYRLGSPVAANAVEK
                     SLADLKTTDNAKDTLERTLAHLKDNRVDLASARLTVGPVLPFDPKSETFPGNEAANKM
                     LTREYRAPYTVPAAGQV"
     gene            9592..>10143
                     /locus_tag="VFB80_13350"
     CDS             9592..>10143
                     /locus_tag="VFB80_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HZN34806.1"
                     /translation="MPGFDILYEDNACLVVAKPPGLLTQAPLGIDSLEFRIKRFLKER
                     GNKPGGVYLGVPHRLDRPVSGVIVFAKNVRAARRISEQFEGRLVEKRYWALVGGEVSP
                     DEGTWTDQLKKIEGEPRSVIVDHTDPAGKLAILHYRVLQFVARDSIAGPATQSPATLL
                     EIDLETGRTHQIRVQAASRGHPLL"
BASE COUNT         1778 a         3255 c         3374 g         1736 t
ORIGIN      
        1 aagaacagca gggcgtctgg agcagcctgc tggccggcga cttcgacccg gcgaagctcg
       61 gccagacgaa attcaaatcg ccggatctca ttggcggccc gggacagtgg gtgaatgggc
      121 agccgctgac cggccagcag ctgcgcggcc gcgtcgtggt cgtgcactat ttcgcgttcg
      181 gctgcatcaa ctgcatccac aactatccca cgtaccgcaa gtggcaggac gagctggcgg
      241 gcaagaacgt ccagctcgtc ggcatccaca cgcccgaaac ggccggcgag cacaacgccc
      301 tgacgctgaa gtcgaagctc aaggccgagc ggctcgattt tccggtgctg gtcgataacg
      361 agaaagccaa ctggacggcc tggggcaaca cgatgtggcc cgcggtttac gtgctcgatc
      421 aggaaggtta tctccgctcg ttctgggccg gcgagctgca ctggcaaggc cgcgacggcg
      481 aggcggtgct ccgcaagcag atcgactcac tgctggacgg gaagtaggtc ttacttgtag
      541 gtccgcctgc cgggcggaac ctgggcagcg cggtgagttc gaatcctcgg caacgctcct
      601 ggtcgctgcg cttccctaaa ggccccttcc ggcagaaggg gcctacttgc tgatctgggc
      661 cgtggcgccg ccgaagtcgg cggggaactg gacttcgtag gccaggctgt cgaggaattt
      721 cctggcagcc aggtagtcgg caggcttgta ggtccgcatc cggccttgca gggccagccg
      781 cagggtatcg atctgctgct gcaggtccac gcagttctgt gaacccgcgc cgctgtcggc
      841 gggcgtccgc cgcacgagca gataatcgat ctgcgaccgc ggttcggaat aatcgatggc
      901 caacagcagc gacggccagc ggacgtagcc cggcttgatc cacagctggt agctgctcag
      961 ccgctcgggt gcggccgagc gggctcgctc ggtgtactgg tcggcggtgc tccgcggcgt
     1021 ctggtgggtg tctttgtagg tgtgatagag ggcccgcttg tcgtaaaaga ccttcgcgcc
     1081 caagaggtcg ttctgcagct tcttctcgcg ggtctcctgg cccatcttgg ccgactcggc
     1141 ctgagcccgc gtgaaagtgg cggcggcggt gatgatcgcc gcgttggctt gcttgttctg
     1201 ggcgtcggcc gagatcactt cggcccgcga tttgtagact tcgatcaggt cgcggttgtc
     1261 ggcgcgggcc gtaccggtca acaaacaggt aatcaggctg gcggtcagcg cggaccggca
     1321 gcgacacagc gaggcaaaca tcggcgcatc cttcgttgtc tggtgggagt ggtgcggccg
     1381 catcctgggg ccgcggcggg cgggtactct gttggaatgc aggagccaag cgtggtcccc
     1441 cggctgatgt tactccaatc gataagcggc tcggcgtaac tgctggctga caagtagctt
     1501 acggcgcgag gtttattctt tgccgggcca gatttcgccc gattctgcgg aagaatcgtt
     1561 gaccgctata atgcggccgc tagcgatctg catgagccac ctcgatcggg agtcgcgcca
     1621 tgatgaagta cgtcgtggag ttgatcggca cattctttct agtgctcacg atcggcaact
     1681 gcgtgcttgc gcccaacgat gcggtgggca acatggcccc gctggcgatt ggctcggcgc
     1741 tgatggtgat gatcttcgcg ggcggccacg tctcgggcgc gcactacaac cccgcagtga
     1801 cgctggcggt gttcctccgt ggcaagtgcc cggctgcgga tgtgctgccc tacatagcgg
     1861 cccaagtcgt cggggcagcc gcggcggcgg cgatcgtgct ctacctcaaa gggctctcgg
     1921 ggattccgcc ggccacgccg atcccggcgg acaatatctt gcgggccatc gtcgccgagt
     1981 ttctctacac cttcgcgctg tgctacgtcg tgctgaacgt cgccacggcc aaaggcacga
     2041 gcggcaactc gttctacggc ctggcgatcg gcttcacggt gctgaccggc gcgtattccg
     2101 tcggcgcgat ttccggcggc gcgttcaatc cggccgtagc cgtcggcttg atcgtgctgg
     2161 gcaaagtcgc cgccacgagc atctggatct ttctggtcgc gaacttcgcc gccggagcag
     2221 ccgcggcatt cgttttcaag acgatcaacc cgacggacaa gtagctgcct atttggcgcg
     2281 gctgctgggt gcggatctta cggctcggcc tgcgcatacc gctcggccaa ttgccggacg
     2341 atcaccgcca cggcagcccg gcaggaggcg ggggcgagga cttcggcctc gctccccagg
     2401 gccaggacgc gggggacgat ctccaggtcg tggtgggcgg gcacggtgag caccaggtcg
     2461 ccgccgctca gcgtctcgat ccgctggttc gcgtgccagg ggtcctcgac gacccaccgc
     2521 gcgcccttgg cggagatgcg aatcttgaag tcgcgggcct ttccgccgga aaagatgccg
     2581 acgccctgcc ccaggtgctc ctccaggtca aaatcctccg gccgatgaaa aaagacgctc
     2641 tgcgccgtgg cccgcagaaa gcgatcgagc ttcaggtgcc gaatccggtc ggcgtggtcc
     2701 gccggcagct cgcgggcggc ggccacgatg tacaggctcg attgatagaa caccacggcg
     2761 tagggctcga tctcgcgctg ctgcggctcg cggcccgtcg gctgatactc gatcgccagc
     2821 acccgccgct cgcggatcgc ccggtcgatg gtggacagga tgccgtgctg ctgctgatag
     2881 ctcttggccg gcatgccgcg gacatagagg acctggcgat aggcctcgta gtgcttccag
     2941 accgacggcg tcagcccgtc cttgagcttg tcccagaacg actccagcgc ctgccagaag
     3001 ggcgtgccgg ccagcggata gagcaggtcg cggccgatcg agagcgcgat cagctcggtg
     3061 gccgaggcct ggatcggatg ggccttgcga gatttccttc ccagccgcca gaccttgccg
     3121 cggcgcaggt actggccttc gatgtcgaag cccgacgcga gcagcgcctc caagtcgcgc
     3181 ttcaccgtcc gcaggtgcag cgacggcagg cccagctcct gggccatgcc gtcgcgcagc
     3241 tccagggtcg tccagccgat ccgcgtccgc tccaaaatct ggagcagctt gtgctgccgg
     3301 acgagctgct cgttgcgaga cataggaatc taggatttag aatctgggat ttgggattgc
     3361 gggttcaaat ccccaagccc aaatcccaaa tctgaaatcc ctattcgaac tttccctcca
     3421 gcgccagctg gtggaaatag tgcggcacct ggtcctggtt ttcgagcagc cagtcgatcg
     3481 agggctcgcc gtgcagggcc ttgtgaatgg ccttggcggt ggcttcgcgg ttggtctgaa
     3541 agaggatgtt gaaatcgacg ttctcggcgt ccaggatgcc cgggtcgagc cagaccgaga
     3601 caatgatgcc gaggtcatcg accttccgcc gcggaatgtc gccggcccgg acggcgtcga
     3661 gcacgccgtg ggcgatggcc gcctgcaccg tccccatgag aatgttcgtg tacttgctgc
     3721 tcttgacggt caccttgctg accatcacgg tcaccggccg caccatcacg tcgctgttca
     3781 ggatggcaaa gaccttgctg tgccctttgg cctggtcgcc gatcaggctg gcgatcgcat
     3841 agcccacggg cccgtccaac tcgccgatca cgacctccgg ctccgccgcc gaccacgccg
     3901 cctccgcctc gaccagtgcc tcgcccgcgc ggagcacgat ccgtttgcgt ttcttgccgt
     3961 ccttgtcttt ctgctttttc ttagccatgg tggacaatca aaatcgaaga atcgaagaat
     4021 cgaagaaccg aagaaccgaa gaaccgaaga accaaataca aaacaccaaa cgcataccct
     4081 cgccagtcat ggacgaacgc gacatcatac agcaaaccgc cgagcacgtg cgggcgctct
     4141 tgtcgggcga agggacgggc cacgactggc accacatcga gcgcgtccgc cgcgcggcgc
     4201 tagccatcgg ccagggcgaa ggcgcggatt tgttcgtcgt tgagctggcg gccttgctgc
     4261 acgacattgc ggattggaag ttcgccggcg gcgatcacga ggcgggcccg cggcagtcgc
     4321 gggcctggct ccagtcgctc ggtgttgacg cgcgggcgat cgaccacgtc tgcgagatcg
     4381 tcgccggact ctcgttccaa ggcgcgggag tgaaggcggc ggtcctgacc ctcgaagggc
     4441 agtgcgttca ggacgccgac cgcctcgacg cgctgggcgc aatcggcatc gcccgcgcgt
     4501 tcgcttacgg cggccacaag gggcagccgc tgtacgaacc cgacgccccg ccgacgcctc
     4561 acgattcgtt cgaggcctac cagcgcggcg cgagctcgac gatcaaccac ttctacgaaa
     4621 agctgctcct gctgcgcgat cgcatgaaca ccgccgcggg ccgccgcctg gccgccgagc
     4681 gtcaccgctt tttggaggct tatctcgagc agttctacgc cgagtggaac ggccagcggt
     4741 aagtcccgcc gcggtcagat cttgcgccga ttcgccgcgc gattggccag tcggaagcta
     4801 gagaaagccg cggtcggcct tcattttctt cgaaatagtt gcaactcgcg ccccgctctg
     4861 gctttgcaac gcggccccag gccacaagtc ccacggtatt caggaattct ttatgacatt
     4921 tgcgtataat atgtgtacgg tcgctacgat gagagacccc gtgcgtttca ccatcgcgga
     4981 cctgctggtc gccacgtcga ttgccggcgt aggcgtcggc gcgctcgtag ccgccgatcg
     5041 gtggtttaac gtgcccgcct gggttcatct tgccgcggcg cccgtcgtgg tgctcagcac
     5101 ggtgggttgg ctgctgggtc agtttcgcgg agcccttgtg ggcgcggtca tcggcttcgc
     5161 catcggactg ttcgcgacga tcgcggctgc ggtcctgcat ggtggttcct cacgcggtga
     5221 ggatccagtg ctgccatcgg ttttccatca ggcacgaatc ccgcgcgcgt ccagccatgc
     5281 ccgctgctgc tacctgacgg cgtcggggag ctacgattcg gataggtaag gataattccc
     5341 gtggatcccc tcaaaccctt ctaacgcagt cgtctctgcg gcccacgaat cgcgacgtaa
     5401 ccgacgcggc atcaaggact tggaaaaagc cgatgaagtc acgaggcaag ttcaaacgcg
     5461 gcccgctttg ggaattatcc ttaccccgcg ccgcgccgat tccagagcca gccaaccgct
     5521 gctgacgcac caccggaccc ccgctagaat ccacggttga agcaccctac gccgccaact
     5581 tatggccatt ccagccggtt tcgagcacat cactcgcgaa aacgagccgc tggcgccgta
     5641 cacctggttc aggctgggcg gggcggccca gtacttcgcc gagccgacca gcgtcgagga
     5701 gctgacgggg ctcgtccgcc gctgccacga gatgggcctc ggcgtgcgcg tgctcggcgg
     5761 gggctccaac ctgctggtcc gcgacgaggg gattccgggg ctggtcgtct cgctcggcgc
     5821 ggccgccttc ggccggattc aagtcgccgg acgcaagctg acggccggcg gcggggcgaa
     5881 gctggggcac gtgatctcga ccgcggtccg cgaagggctc gccggcctgg aatcgctcgt
     5941 cggcattccc ggcacgctcg gcggcgcgct gcacaccaac gccggtacgc tgggtggcga
     6001 catcggacaa ttcacgacga cggctacggc gatgaccggc aagggcgaga ccgtgactcg
     6061 gcaaaagggc gagttccgct tcggctaccg ggccagcagc ctcgacgagc tggtgatcct
     6121 cgaggccacg ctggaattgg agcccagcga ttcgcggcac ctcaccaagc agatgcagca
     6181 ggcctggatc atcaagcggg ccgagcagcc gctgttcgac cagaagaccg gacagatctt
     6241 caagagcccc ggcggcgtca gcgcggcgag cctgatcgag gacgccaggc tcgataccgc
     6301 ccacgtcggc gacgcggcga tcagcgaccg caacgccaac ttcatcgtcg tgggtgccag
     6361 cgccaccagc cgcgacgtga tcgagttgat cgaggtggtc cgcaagggag ttgcggagcg
     6421 ggtcggcgtg gagttggaat tggctgtgga agtctggtaa cgaaatctaa gaaccaaaga
     6481 accgaagaac cgaagaacca aacagggagt cagctctttg gttcttggat ctttggttct
     6541 tcgatttttc ccgccgaaac ggagttcgga cgatgggaac gaacaagccc aagacgccgc
     6601 ggccctcgcc ggtggcggca caactggtag cggcgctgct taagccgcaa aaccgcggcc
     6661 tggttttgac ggcggcgatc gtggttgccg ccgtctgcgg cgcggtgtac gcctggcagc
     6721 gctttggcgc agcggcgttg aattcggccg actacgccgt gacgccggcg cagatcgccg
     6781 tcacgccgca gccggcgtgg attcacgcgg atgtgaaagc cgaggcgctg cggtcggcgt
     6841 cgatgtcgcg gctgaatctc cgcgaccgcg atctggtaga gaaggtcgcg cgggccttcg
     6901 ccctgcaccc gtgggtcgcg aaggtcgtgc gcgtcgaaaa gcgcaatccg ccgcaggtga
     6961 gcgtcgagct ggaatatcgc cgcccggtcg ctgcggtcga agtttccggc ggcggcgagg
     7021 ccggcctggt tttcatcgac gagcagggcg tgctcctgcc ctcggccgat ttcgcgccgg
     7081 gacaggccaa ggactacttg cgaatcgccg gcggccgcga gacgaccgcc agcgtttatg
     7141 gttcgccctg gggcagccag cggatcgctg gcgcggcccg gctggccgcc ctgtggggaa
     7201 agcgctggca gccgctgggt ctgttccgca tcgacgccga gcagccgccg ggccaggagc
     7261 tgaattatga gttgaaaacc cgccgcggcg tgcgcgtgat ctggggtccg atcgctggat
     7321 catcccgcga gccgacggcg gagcagaaga tcgccgcgct ggagcaattc atcagcgaca
     7381 agggccccct ggaacgcgag gggggaccgg ccgttttgga cttgtgcgaa ctggcgcggt
     7441 gagggggcag cgagcgggat taagattagg attaggatta agattaggac tcggctgcgg
     7501 atcctaatcg taatcctagt cctaccgcgc tagccctctc ccaaagggag aggcgacgtc
     7561 gcctcggtgg cggtggcttc gccctcggcg gtccgctcgg ccagttcctg gcggacgacg
     7621 accatgtcag ccggggcctc gatccccagg cgcaccgcgt tcccgttgat tcgcacgatc
     7681 gtgatcgcga tgcggtcgcc gatcagaact cgctcgccca ctttgcggct gaggaccagc
     7741 atggcagcac tccttgccca aacggggact gaaccacccg ttgcaatgcc tccgaaatgc
     7801 actccgcgat gccttggtac gttctatcag agctagcggg cacccgcaat cagccgggcg
     7861 gcaacccgct tagccccggc gtccacgccg gtcgagcggg gtgaccgctg gccaaaccgg
     7921 cctgggggcc ggggctaagc gagattggca attcgggcaa ggctgctgga aagccgttac
     7981 ggcggcgggt tattatgagc cggcgcacat ttctctccct ggggcaggag ctaccgaaaa
     8041 tggccagaag aaaccgccgt caattcttgc aagattcgat gtttgccacg gcagcagccg
     8101 cgatggcgag ttccacgggc cggtatgcca cggcccagga ccagccggcg agcaagagcc
     8161 ccaacgagcg gttgaacgtg gcctgcgtgg gcgtcaaggg ccgcggcggc agccacatca
     8221 gcgcctgggc ggctaagccc gacacgatgg tgacctacgt cgtcgatgtg gacagcaaga
     8281 tcggcaacgg ccgggtcgat gagatcgaga agcggcaggg cgtgcggccg aagctggtcg
     8341 atgacattcg cgacgtgctg gaagacaaga gcgtcgacat catcagcgtg gccacgccga
     8401 accactggca ctcgctgatg gcgatctggg cgatgcaggc cggcaaggac gtgtacgtcg
     8461 agaagcccgt gagccacaac gtcagcgagg gccgccgcgc agtggaaacg gcccgcaagt
     8521 acaagcggat ttgccagacg ggcacgcagt gccgcagcaa ccccggcatg cggcagctca
     8581 tcgagtacgt ccagtcgggg aagatcggcg cggtgaaggt cgcccgcggc ctgtgctaca
     8641 agccccgcgg gtcgatcggc gcgaagggca cctacgaagt ccccgcgtcc gtcaactacg
     8701 acctgtggtg cggcccggcc cggatgctgc ccgtgacccg cccgcagttc cattacgact
     8761 ggcactggat ttgggagtac ggcaacggcg acctgggtaa ccagggcatt caccagatgg
     8821 acatcgcccg ctggggcctg ggcgtgaacg agctctgcaa gtccacgttc agctacggcg
     8881 gacggctggg ctatgaggac gccggcgaga ccgccaacac gcaggtctgc gtccacgact
     8941 tcggcgacaa gtcgctggtg ttcgaggtcc gcggcctgcg gaccgaccgc tacaagggcg
     9001 ccggcgtcgg cgtgatcttc gaatgcaccg agggctacgc cgtctcgccc agctacaacg
     9061 gcggcacggt gttcgacaag gagggcaagg agatcgcccg cttcagcgag ggcgaggaca
     9121 agtatcactt cgccaacttc ctggccgccg tccgcacgcg gaagctgacc gacctgaacg
     9181 ccgacatcga agaagggcac ctgtcgagcg ccctctgcca cctgggcaac atctcgtacc
     9241 ggctcggatc gccggtggcg gctaacgccg tggagaagtc gctcgccgac ctgaagacga
     9301 ccgacaacgc caaggacacg ctcgagcgga cgctcgcgca cctgaaggac aaccgcgtcg
     9361 atctggcctc ggccaggctg accgtcggcc cggtgctgcc gttcgatccc aagtccgaga
     9421 ccttccccgg caacgaagcg gccaacaaga tgctcacccg cgagtatcgc gcgccataca
     9481 ccgtgcccgc ggccggacag gtctaggtag cttcctcacg ccgtgaggaa gcaattcgcc
     9541 aacggcgcca ccgcgtgcgc tagccccccg acggcgtcgg gggcctactt tatgcccggc
     9601 ttcgacatcc tctacgagga caacgcctgc ctcgtcgtgg cgaagccgcc gggattgctg
     9661 acgcaggccc cgctgggcat cgacagcctg gagttccgca tcaagcggtt cctcaaagag
     9721 cgcggcaaca agccgggcgg agtatacctg ggcgtgccgc accggctgga ccggcccgtg
     9781 tcgggcgtga ttgtatttgc gaagaacgtc cgcgcggctc gccgcatctc cgagcagttc
     9841 gaagggcggc tggtcgagaa gcgctattgg gcgctggtcg gcggcgaagt atcgccggac
     9901 gagggaacgt ggaccgatca gctcaagaag attgagggcg agccgcggag cgtgatcgtc
     9961 gatcacaccg atcctgcggg caagctggcg atcctgcact accgggtctt gcagttcgta
    10021 gcccgcgatt ccatcgcggg acccgcgaca cagtcgccgg ccacgctgct ggagatcgat
    10081 ctggaaaccg gccgcacgca ccagattcgc gtgcaggccg ccagccgcgg ccatccgctg
    10141 ctc
//
LOCUS       DASRJB010000510         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_425138, whole genome shotgun sequence.
ACCESSION   DASRJB010000510 DASRJB010000000
VERSION     DASRJB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425138"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /gene="ettA"
                     /locus_tag="VFB80_13355"
     CDS             complement(<1..1407)
                     /gene="ettA"
                     /locus_tag="VFB80_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZN34807.1"
                     /translation="MSRQYIYTIERLTKKHGQREVLKDIWLAFYPGAKIGVLGRNGSG
                     KSTLLRIMAGVEKDIDGEARLSDGFTRGFLSQEPQLSPGTVWDNLQEAIKPRRAEVDR
                     YNEITTLLAEPMDDEQMQKLCDEMARLQDHIETTGAWELDRELEIAMDAMNLPDREAD
                     SQTLSGGERRRVALCKLLLNKPDLLLLDEPTNHLDAEAVNWLEHHLAEYHGTVVAVTH
                     DRYFLDNVAQWILELDRGMGFPFEGNYSSWLEQKKARLEREEKSNVAKQKTLQRELEW
                     VRMSPRARQAKSKARIAAYEKMSAERFEERQDEFEIQIPPGKHLGELVVEAKGLTKGY
                     GDRVLIDRLDFRLPAGGIVGIIGPNGAGKTTLFRMIVGQEKPDGGELRVGPTVELGYV
                     DQNRDALAADKTAYEEISGGHDNLEMGGRKVNSRAYVNRFNFRGPDQEKKVGTLSGGE
                     RNRVHLAKLLRRGSNLLLL"
     gene            complement(1567..2235)
                     /locus_tag="VFB80_13360"
     CDS             complement(1567..2235)
                     /locus_tag="VFB80_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HZN34808.1"
                     /translation="MALFEIVAPAGLKMIRCVIENEMVRAESGALHYMRGNIELTSAA
                     PSVGGFLKSVMTRENIFRPTYQGTGEIFFGPPTFGEYEILELKQEEWILEQGSYVCSD
                     ASIEVGVWRNKALTALFGGEGWFQTSVKGTGKVVMQAPGRVQRIDLRGEKLSVDGKFA
                     IARTASVNFEVQKAAKSILGSLSSGEGLLSTFTGHGTVLIAPVPNTYQSLLASITQAI
                     PRGK"
     gene            complement(2323..>3043)
                     /locus_tag="VFB80_13365"
     CDS             complement(2323..>3043)
                     /locus_tag="VFB80_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN34809.1"
                     /translation="SFRERYRREMSCQVVHDSIHRRAGWMLIYRLALDGAAAGFGSVA
                     VGGPWKDKPTLLEFYVLPESRWRAFELFEALLAASGAELVEMQSNDALFAVMLHTYAG
                     QIASEKIVFHDQLTTSLPACGAALRRVTSAEETQAHIEQRQGGIECVLELDGQVIGQG
                     GVLFHYNRPYGDIYMEVAEPFRRRGLGSFLVQELKRVAYDLGAVPCARCSPANIASRR
                     TLQKAGFVPFAHMLTGIIAQP"
BASE COUNT          506 a          996 c          953 g          588 t
ORIGIN      
        1 gaggagcagc aggttgctgc cgcggcggag gagcttggcc aggtggacgc ggttgcgttc
       61 gccgccggag agggtgccga cttttttctc ctggtcgggc ccgcggaagt tgaagcggtt
      121 gacataggcc cgcgagttca ccttgcggcc gcccatttcc aggttgtcgt ggccgcccga
      181 gatttcttca taagcagtct tgtccgccgc gagggcgtcg cgattctgat cgacgtagcc
      241 cagctcgaca gtcgggccga cgcgcagctc gccgccgtcc ggcttttcct ggccgacgat
      301 catgcggaac agcgtcgttt tcccagcacc gttagggcca atgatgccca cgatcccgcc
      361 cgccggcagc cgaaagtcga gcctgtcgat cagtacgcgg tcgccgtagc ccttggtcag
      421 ccccttggcc tcgacgacca gctcgcccag gtgttttccg ggcggaatct gaatttcgaa
      481 ttcgtcctgg cgttcctcaa aacgctcggc cgacatcttt tcgtaggccg cgatgcgggc
      541 tttgctcttg gcctggcggg cgcgggggga catgcggacc cactccagct cgcgctgcag
      601 cgtcttctgc ttggcgacgt tcgacttctc ctcgcgctcc aggcgggcct tcttctgttc
      661 cagccacgag ctgtagttgc cctcgaacgg aaagcccatc ccacggtcga gctccaaaat
      721 ccactgggcc acgttgtcga ggaagtagcg gtcgtgcgtg acggcgacga ccgtgccgtg
      781 gtactcggcc aggtggtgct cgagccagtt gacggcttcg gcatcgaggt ggttggtcgg
      841 ctcgtccagc agcagcaggt ccggcttgtt gagcagcagc ttgcagaggg cgactcgccg
      901 ccgctcgccg cccgaaagcg tctggctgtc cgcctcgcgg tcgggcaggt tcatcgcgtc
      961 catcgcgatt tccagctcgc ggtcgagctc ccacgccccg gtcgtctcaa tatggtcctg
     1021 gagccgggcc atctcgtcgc acagtttctg catctgttcg tcatccatcg gctcggccaa
     1081 gagcgtcgta atctcgttgt agcgatcgac ctcggcccgc cgcggcttga tggcctcctg
     1141 caagttgtcc cagaccgtgc cgggcgagag ctgcggctcc tgcgacaaaa acccgcgcgt
     1201 gaaaccgtcg ctcaagcggg cttcgccgtc gatatccttt tcgacgccgg ccatgattct
     1261 tagcagcgtg ctttttcccg acccgttgcg gcccagcact ccgatcttgg cccccggata
     1321 gaacgccagc cagatatcct tcagcacctc gcgctggccg tgcttcttcg tgagccgctc
     1381 gatggtgtag atgtactgcc gcgacatggg cgggaaaacc cgaagaacca aagaaccgaa
     1441 gaaccaaaaa tcagtctagc ggtcgcccgg attcgccgca gggggacgtg cagttgcggc
     1501 gccgtgacga gcactcttaa catcacttct ttcggttctt tggatcttcg gttcttgggt
     1561 tcttcgtcac ttcccgcgcg ggatggcctg agtaatcgag gcgagcagac tctggtacgt
     1621 gttggggacc ggcgcgatga ggacggtgcc gtggccggtg aaggtgctga gcaggccttc
     1681 gccggaggag agggagccga gaatcgattt ggccgctttc tgcacctcga aattcacgct
     1741 ggcggtgcgg gcgatcgcga acttgccgtc caccgagagc ttttcgccgc ggaggtcgat
     1801 tcgctggact ctgcccgggg cctgcatgac cactttgccg gtccccttga ccgaggtttg
     1861 aaaccagcct tcgccgccga agagcgcggt cagggccttg ttccgccaga cgccgacttc
     1921 gatcgaggcg tcgctgcaaa cgtacgagcc ctgctccaga atccactctt cctgcttgag
     1981 ttcgagaatc tcgtactcgc caaaagtggg cggaccgaag aagatttcgc ccgtgccctg
     2041 ataggtcggg cggaagatgt tctcgcgcgt catgacgctc ttcaggaagc cgcccacgga
     2101 cggcgctgct gacgtcagct cgatgttgcc ccgcatgtag tgcagcgccc ccgattccgc
     2161 ccgcaccatt tcgttctcga tcacgcagcg aatcatcttc agcccagccg gtgccacaat
     2221 ctcgaacagc gccatgccac gccctccgca attctcagaa atgtcgccag acgccgcacc
     2281 gtgattcgcc cggccggggc gaatattggc ggcagaacgc cgctacggct gtgcaatgat
     2341 gccggtgagc atgtgggcga agggcacgaa gccggccttt tgcagcgtgc ggcgcgaggc
     2401 gatgttcgca gggctgcacc gggcgcaggg aacagcgccg aggtcgtagg cgacccgctt
     2461 cagctcctgg acgagaaacg aacccagccc gcggcggcga aatggctcgg ccacctccat
     2521 atagatgtcg ccgtatggcc ggttgtagtg aaacaggacg ccgccctgcc cgataacctg
     2581 gccgtccagc tccagcacgc attcgatccc gccttgccgt tgctcgatgt gggcctgcgt
     2641 ctcctcggcc gaggtgacgc ggcgcagcgc cgcgccgcag gcaggcagcg acgttgtcag
     2701 ctggtcgtga aacacgatct tctcgctggc gatctggccg gcgtaggtgt ggagcatgac
     2761 ggcaaacagc gcgtcgttcg attgcatctc gaccagctcc gcgccgctgg cggccagcag
     2821 cgcctcgaac agctcgaagg cccgccagcg agattccggc agcacgtaga actcgagtag
     2881 cgtcggcttg tccttccacg gcccgccgac agcgaccgag ccaaatcccg ctgccgcacc
     2941 atccagtgcc agccggtaga tcagcatcca gcccgcgcgg cgatggatgg aatcatgcac
     3001 cacctggcag ctcatctcgc ggcggtatcg ctcgcggaag gaa
//
LOCUS       DASRJB010000511         8064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3242981, whole genome shotgun sequence.
ACCESSION   DASRJB010000511 DASRJB010000000
VERSION     DASRJB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242981"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFB80_13370"
     CDS             complement(<1..833)
                     /locus_tag="VFB80_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34810.1"
                     /translation="MFPPTNRRHFLLTASSTALAAAAVTAPAAAQSANNKLVVGLIGC
                     GGRGTHDAGLFQKTTNVEVACVCDVDDGRRNLAAKNLGIAAGKAVSDMRRILDDKSVD
                     AVIVATPDHWHSPAAILACEAGKHVYVEKPISHNIREGRLLVESSQRNKTHVQHGTQS
                     RSTAMMIQAVQLLREGIIGNVLVAKCWNIQRRGSIGRGTDTAPPAGFDYDAWVGPATM
                     IPYRTNRVHNRWTWWYHFGTGDMGNDGVHDIDYTRWGLGVETHPSKIVALGGKYVNDD
                     D"
     gene            923..2209
                     /locus_tag="VFB80_13375"
     CDS             923..2209
                     /locus_tag="VFB80_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN34811.1"
                     /translation="MLLRSPFVCLGLVLLAGRCPAATVVHAGRLIDGRSDQPRMEVSI
                     VIDGGRIVRVAAGYLKPAADDKLVDLREYTVLPGLMDMHTHLQSQHSKESYTERFFMD
                     QADYALRSTLHARRTLLAGFTTVRDLGDNGVNSIALRKAIEQGWAMGPRIFTSGKSLA
                     TTGGHADPTNALRGDYRRDPGPLEGVINGADDARKAVRQRYKDGADLIKLTATGGVLS
                     LAASGQNPQFTDEELRAVVATAKDYGMIVAVHAHGTEGIKRAVLAGVDSIEHGTYMTD
                     EIMELMKQRGTYWVPTNMAGEWVAKQAEVPGYFPEIVRPKAAAIGPAIRETFKKAQAA
                     GVKIAFGTDSGVSPHGENAHEFELMVAGGMPPMKAIQSATLVAAQLLRIDDRLGTVEE
                     KKLADLVAVKGDPLANISLLREIAFVMKDGIVFKGP"
     gene            complement(2286..3065)
                     /locus_tag="VFB80_13380"
     CDS             complement(2286..3065)
                     /locus_tag="VFB80_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN34812.1"
                     /translation="MDKNWAADFLGRLSASWHLLGVSLRRLLWSRQTIISAVLVVLAG
                     VAAYAWARDRQRQPSDFVEEVLLTVYVPFLLPILCLSYATAGIATDRDEQTLVYLVTS
                     PLPRPFIFLAKAAAALVLSLAWTMGTLALLAGIAGKPGLEVFRAVWPGLFWATVAYVC
                     LFQVFSVAFRRATIYALGYALFLETLIGNMPGIVKRLAISFYARCLIFDASAAFDVGP
                     SGPFNELLFQPLPAATAYTILYAASAGLLLLGLVIFCTREY"
     gene            complement(3068..4021)
                     /locus_tag="VFB80_13385"
     CDS             complement(3068..4021)
                     /locus_tag="VFB80_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN34813.1"
                     /translation="MTGAPIPQPLCRFQAASKWYGPVIGLNDLTWDLPPGITGLVGHN
                     GAGKSTLIKLLTGQLRPSLGRVLVRGHRAWTAAARRHIGYCPDVDAFYEEMSGRAFVR
                     MLARLHGFSSKTARERTDDVLDQVDMAERADRPLGSYSKGMRQRIKLAQALVHNPDLI
                     VLDEPLNGVDPVGRVELIRLFRSLADRGKSLLISSHILDEMDTLADQILFICRGRMLA
                     SGSLDEIRALLDDHPLKVRIAAPRARQLAAELLRLESVRSAALESEGQLLLEVQRPKE
                     FFGPLAAAVAEQACDVQRLQVIDASTEAIFDYLMETAKRPR"
     gene            complement(4018..4899)
                     /locus_tag="VFB80_13390"
     CDS             complement(4018..4899)
                     /locus_tag="VFB80_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34814.1"
                     /translation="MSVDTAGYRRWAGRQLPNALACWPIARSGLVLVLRRKLFWLLLA
                     LAALHFLFIFATIYLKFQIAAQNPGLERFVNQVLRSVTGTGETYREFMFRQGTVTMLV
                     LAFAGGMLVGDDMRRGGLTFYLSRRIGRWHYVVGKLLAIGLLVSLTTTLPALVLYIEY
                     GLLTNSFSYFRENLGILVGILGYGLVMAVTLALLLFALAAWLHKTVPLVMAWACLFVL
                     LPALGELLQDVFDDRHWLLLSLWRDIRLVGTWFFGGIDTERELAMLNPSLAIVLGVCI
                     VSTLALVPRVRAVRVVQ"
     gene            complement(4896..5840)
                     /locus_tag="VFB80_13395"
     CDS             complement(4896..5840)
                     /locus_tag="VFB80_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN34815.1"
                     /translation="MSLLLELHEVTHFYGRVCALSGVTLNVEPGPIGLVGQNGAGKST
                     LLQILLGLIRPTHGTAVVLGQNVRTAGLALRGRVGYMPEHGGLVTGLAGVEYVALAGE
                     LCGMPRKQALRRGHETLSQLGLEDARYRPLDQYSVGMVQRLKLAATLVHDPDLLLLDE
                     PTSGLDPEGRTAMLHLLESLASRPGKSLLLASHLLGDIERVCQSAIILRQGQVLKVGR
                     IDELRTTRPRSFRLRWDGDAAPYLAGLRERGVQIELDGKPGQAKALVPTDWQTREFFA
                     EAHRQSLPLTGLEPEEEDLQAVYQRLMTAPPEPAGRTA"
     gene            5956..6888
                     /locus_tag="VFB80_13400"
     CDS             5956..6888
                     /locus_tag="VFB80_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN34816.1"
                     /translation="MTRRISFAIAVIACLSSAHLAAADEPQVIELWPAGKVPEEPGTI
                     GAEFTRPSPALGRKDVEVTEPTTLVSNVTRPTLTIYRPAKEMDTGTAVLVCPGGGYWN
                     LYWQLEGEEVAKWLNTIGATGILLKYRVPRRPDEPRGQPARRPLQDAQRAVSLVRSRA
                     KELGIHPERIGLVGFSAGGHLAIATATSFEKRTYEPVDEIDQVSCRPNFAIPVYSGYL
                     KAQDKDELAPGLAVPKETPPVFLVHGGDDIISPPEGTVIMYLALKRAGIPAELHIYAR
                     TAHDFGVRQVDRPYASWTDTCARWLKDQGLLKKE"
     gene            complement(7312..>8064)
                     /locus_tag="VFB80_13405"
     CDS             complement(7312..>8064)
                     /locus_tag="VFB80_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34817.1"
                     /translation="MFGFVDVIEIHPPQGIFTPPQKQADGKLERNPTCHWMQMLNLGY
                     RHPGVINTDSHYNYHESGFFRNFIHSSTDDPARIGVMEMVHNAEAGHVVVSTGPFLTV
                     DAVGDSLGKEVGAMPGMDLPATGGRVTLHVRVQCPNWFDINRVQVFANGRPLPALNFT
                     RRLAPDRFSDKTVRFDAKIPVEVKADTHLIVAAIGEGLELGPVMGPTYGKFPPVAVAN
                     PIFVDVDGGGFKPNGDMLDVPLTVDPNRPKAN"
BASE COUNT         1462 a         2654 c         2712 g         1236 t
ORIGIN      
        1 tggtcgtcgt cattgacgta tttgccgccg agggcgacga tcttcgacgg atgcgtctcc
       61 acgccgagtc cccagcgggt gtagtcgatg tcgtggacgc cgtcgttccc catgtcgccg
      121 gtgccgaagt ggtaccacca ggtccagcgg ttgtgaacgc gattggtgcg atacggaatc
      181 atcgtcgctg ggccaaccca ggcgtcgtag tcgaagccgg cgggcggagc ggtgtcggtg
      241 ccgcggccga tcgagccgcg gcgctggatg ttccagcact tggcgaccag cacattgccg
      301 atgatcccct cgcggaggag ctgcacggcc tggatcatca tcgcggtgct gcggctctgc
      361 gtgccgtgct gcacgtgagt cttgttccgc tgcgacgact cgaccagcag gcggccctcg
      421 cggatgttgt gcgagatcgg cttttcgaca tagacgtgct tgccggcctc gcaggccagg
      481 atcgccgccg gcgaatgcca gtggtcgggc gtcgcgacaa tcacggcatc gacgctcttg
      541 tcgtcgagga tgcggcgcat gtcgctcacc gctttgcccg cagcgatgcc caggtttttg
      601 gccgccaggt tgcgccgccc gtcatccacg tcgcagacac aggccacttc gacgttggtc
      661 gttttctgaa acagccccgc atcgtgcgtg ccgcggccgc cgcagccaat caggccgacg
      721 acgagcttat tgttcgcgct ctgagcggca gccggtgcgg tgaccgcggc agcggccagc
      781 gcggtggaag aagccgtgag caggaagtgc cggcggttgg tgggaggaaa catggctggg
      841 ctcctggaga agacgggatg cccgctcatt gtagagacgg cagccggccg caggtaacat
      901 ggggccacgg gagaccaaca ccatgctgct ccgctcgcca ttcgtctgcc tgggtctcgt
      961 tcttctggcc ggccgctgcc ctgccgctac ggtcgttcac gccggccgcc tgatcgacgg
     1021 ccgcagcgat cagccgcgaa tggaggtttc gatcgtgatc gacggcgggc ggatcgtgcg
     1081 ggtggccgcg ggctatttga agccggcggc ggacgacaag ctggtcgatc tgcgcgaata
     1141 caccgtgctg cctggcttga tggacatgca cacgcacttg cagtcgcagc attcgaagga
     1201 gtcgtacacc gagcggttct tcatggacca ggccgattac gccctccgct cgacgctgca
     1261 cgcccgccgc acgctgctgg ccgggttcac gacggtccgc gacctgggcg acaacggcgt
     1321 gaactcgatc gcgctccgca aggcgatcga gcagggctgg gcgatggggc cgcggatttt
     1381 cacgtcgggc aagtcgctgg ccacgaccgg cggccacgcc gatccgacga acgcgctccg
     1441 cggcgattat cgccgcgatc cggggccact ggagggagtg atcaatggcg ctgacgacgc
     1501 ccgcaaggcc gtgcggcagc ggtacaagga cggcgcggac ctgatcaagc tgacggcgac
     1561 cggcggcgtg ttgagcctgg ccgcgagcgg gcagaacccg cagttcaccg acgaggagct
     1621 gcgggcggtc gtcgcgaccg ccaaggatta cggcatgatc gtcgccgtgc atgcccacgg
     1681 cacggagggg atcaagcggg ccgtgctggc cggcgtcgat tcgatcgagc acggcacgta
     1741 catgaccgac gagatcatgg aattgatgaa gcagcggggc acgtactggg tgccaacgaa
     1801 catggccggc gagtgggtcg cgaagcaggc cgaggtgccc ggctattttc ccgagatcgt
     1861 gcggcccaag gcggcggcga tcgggccggc gattcgcgag accttcaaga aggcccaggc
     1921 cgccggcgtg aagatcgcct tcggcaccga cagcggcgtc tcgccgcacg gcgagaacgc
     1981 ccacgaattc gagctgatgg ttgccggcgg gatgccgccc atgaaggcca tccagtcggc
     2041 cacgctcgtg gcggcgcagc tgctgcggat cgacgaccgc ctggggaccg tcgaggagaa
     2101 gaagctcgcc gatctcgtgg ccgtgaaagg cgatccactg gccaacatca gcctgctccg
     2161 cgagatcgcc ttcgtgatga aggacggcat cgtcttcaag gggccgtagc cggacgcaaa
     2221 gccgcgacca gtggtcgcgg ctacctgtgc accgcgacca ctggtcgcgg cttgaggcga
     2281 cgaggtcaat actcccgtgt gcagaagatg acgaggccga gcaacaacag cccggcgctg
     2341 gcggcgtaga gaatcgtata ggccgtcgcg gccggcagcg gctgaaagag cagctcgttg
     2401 aacggcccgg aagggccgac gtcgaaagcg gccgaggcgt cgaagatcag gcaccgggcg
     2461 taaaacgaga tcgccagccg cttgacgata ccgggcatgt tgccgatcag cgtctccagg
     2521 aacaaagcgt agcccagcgc ataaatcgtc gcccggcgaa aggccacgct gaagacttga
     2581 aacaagcaaa cgtaggccac cgtcgcccaa aacaggcccg gccagaccgc gcggaatacc
     2641 tccaggccgg gcttgccggc gatgccagcg agcagggcca gcgtacccat cgtccaggcc
     2701 agcgagagga ccagcgcggc ggcggccttc gccagaaaga tgaacggccg cggcagcggc
     2761 gaggtgacca gatacaccag cgtctgttcg tcgcggtcgg tggcgatgcc ggccgtggca
     2821 taagagaggc agaggatcgg cagcagaaac ggcacgtaaa cggttaacag cacttcctcg
     2881 acgaagtcgc tcggctggcg ctggcgatcg cgggcccagg cataggcggc cacgccagcc
     2941 agcacgacga gcaccgcact gatgatcgtc tgccgcgacc agagcagccg ccgcagcgac
     3001 accccgagca ggtgccagct cgccgagagg cgtccgagga aatccgcggc ccaattcttg
     3061 tccatggtca ccgcgggcgt ttggccgttt ccatgaggta gtcaaaaatc gcttcggtcg
     3121 aggcgtcaat cacttgcagc cgctgcacgt cgcaggcctg ctccgccacg gcagccgcca
     3181 gcggtccgaa gaactctttg ggccgctgca cttccaggag cagttgcccc tcggactcga
     3241 gcgcggcgga gcggaccgat tcgaggcgga gcaattcggc ggcgagctgc cgggcccgcg
     3301 gcgcggcgat gcggaccttg agcggatggt cgtcgagcag ggcccggatt tcgtccagcg
     3361 agccgctggc cagcatccgg ccgcggcaga tgaacagaat ttgatcggcc agcgtgtcca
     3421 tctcgtccag gatgtggctg gagatgagca gcgacttgcc acggtcggcc agcgagcgga
     3481 agaggcggat cagctcgacg cggccgaccg gatcgacgcc gttgagcggc tcgtcgagca
     3541 cgatcaggtc ggggttgtgc acgagcgcct gcgccagctt gatccgctgc cgcatgcctt
     3601 tggaatacga gcccagcggc cggtcggctc gctcggccat atcgacctgg tcgagcacgt
     3661 cgtcggtccg ctcgcgggcg gtcttggacg aaaagccgtg caggcgggcc agcatccgca
     3721 cgaacgcccg gcccgacatt tcctcgtaaa aggcatcgac gtcggggcag tagccgatgt
     3781 gccttcgggc ggccgccgtc caagcgcggt ggccgcggac cagcacgcgg cccaggctcg
     3841 gccggagttg cccggtgagc agcttgatca gcgtgctctt gcccgcgccg ttgtggccaa
     3901 cgagcccggt gatgcccggc ggcaggtccc acgtcaggtc gttcaggccg atcaccggcc
     3961 cgtaccattt gcttgctgcc tgaaagcggc agagcggctg cggaattgga gcgccggtca
     4021 ttggaccacc ctgaccgcgc ggacccgcgg cacgagcgcc agcgtggaga cgatgcagac
     4081 gcccagcacg atcgccagcg acggattcag catcgccagc tcccgctcgg tgtcgatgcc
     4141 gccgaagaac cacgtgccga ccagccggat gtcgcgccac agcgagagga gcagccagtg
     4201 acggtcgtcg aatacgtctt ggagcagctc gcccaaggcc ggcagcagca cgaacaggca
     4261 ggcccaggcc atcaccagcg gcaccgtctt gtgcagccac gccgccaagg cgaatagcaa
     4321 cagcgccagc gtgaccgcca tcaccaggcc gtagcccaga atgccgacga gaatgcccag
     4381 gttctcgcga aagtagctga atgaattcgt gagcaggccg tactcgatat acaggaccag
     4441 cgccggcagc gtcgtcgtca gcgagaccaa caggccgatg gccagcagct tgccgacgac
     4501 gtaatgccag cggccgatcc gccgcgacag atagaacgtc agcccgccgc ggcgcatgtc
     4561 gtcgcccacg agcatcccgc ccgcgaaggc gagcacaagc atggtcacgg tgccttggcg
     4621 aaacatgaac tcgcgatacg tctcgccggt gccggtcacc gaccgcagca cctgattgac
     4681 gaaccgctcc aggccgggat tctgggccgc gatctgaaac ttgagataga tcgtcgcgaa
     4741 gatgaacagg aaatgcaggg ccgccagcgc cagcagcagc cagaacagct tccgccgcag
     4801 caccagcacc aggccgctgc gggcgatcgg ccagcaggcc agcgcgttgg gcaattgccg
     4861 cccggcccag cggcgatagc ccgccgtatc gacgctcatg cggtcctccc ggctggttcc
     4921 ggaggagccg tcatcagccg ctgatagacg gcctgcaaat cctcctcttc gggttccagg
     4981 ccggtcagcg gcagcgactg gcgatgggcc tcggcgaaga actcgcgggt ctgccagtcg
     5041 gtcggcacga gcgccttggc ctgtcccggc ttgccgtcga gctcgatctg cacgccgcgc
     5101 tctcgaaggc cggcgaggta cggcgcggcg tcgccgtccc accgcaggcg aaagctgcgg
     5161 ggccgcgtcg tccgcaattc gtcgatccgg ccgaccttca gcacctggcc ctgccgcagg
     5221 atgatcgccg actggcagac ccgctcgatg tcgcccagca ggtgactggc caggagcagc
     5281 gacttgcccg gccgcgacgc cagcgattcc agcaggtgga gcatcgccgt ccgcccttcg
     5341 ggatccagcc cagaggtcgg ttcgtcgagc agcaacagat cgggatcgtg cacgagcgtc
     5401 gcggccagct tcaggcgctg gaccatgccg acggagtact ggtcgagcgg gcggtagcgg
     5461 gcgtcctcca ggccgagctg cgaaagcgtt tcgtgcccgc ggcgcagggc ctgcttccgc
     5521 ggcatgccgc acaattcgcc ggccagggcg acatattcca cgcccgccag gccggtgacc
     5581 agtccgccgt gttcgggcat gtagccgacg cggccgcgga gcgccagccc ggcggtccgg
     5641 acgttctgac ccagtacgac ggccgtgccg tgggtcggcc ggatcaaccc cagcaggatt
     5701 tgcagcagcg tggatttgcc cgcgccgttc tggcccacca ggccgatcgg tcccggctcg
     5761 acattcagcg tcacgcccga cagcgcgcag accctcccgt agaagtgagt gacctcgtga
     5821 agctccaaga gcagcgacat gcagcaatgt tccgtgcggc ggccgcattc tggaccccgc
     5881 gcgggaaagc ggtcaagatg gcgggcactg tagacctcgc gctccgcgtg aggccgcttc
     5941 gccaaggatc caccaatgac gcgcagaatc tcttttgcga ttgcggtgat cgcctgcttg
     6001 agctcagccc atttggcagc cgccgacgag ccgcaggtca tcgagctctg gccggccggc
     6061 aaggtgcccg aggaacccgg cacaatcggc gcggagttta ctcgaccgtc gccggcgctg
     6121 ggccgcaagg acgtcgaggt caccgagccg acgacgctgg tcagcaacgt cacgcggccg
     6181 acgctcacga tctatcgccc cgccaaggaa atggacaccg gcacggccgt gcttgtctgc
     6241 cccggtggcg gctactggaa tctctactgg cagctcgaag gcgaggaggt cgcgaaatgg
     6301 ctgaacacga tcggcgcgac cggcatattg ctgaagtacc gcgtgccgcg gcggcccgac
     6361 gagccccgcg gccagccggc ccgccgcccg ctgcaggacg ctcagcgggc ggtgagcctc
     6421 gttcgcagcc gggcgaagga gctaggcatt catcccgagc ggatcggcct ggtcggattc
     6481 tccgccggcg gccacctggc aatcgcaacg gctaccagct tcgaaaaacg gacctatgag
     6541 ccggtggacg agatcgacca ggtgagctgc cggccgaact ttgcgattcc cgtttactcc
     6601 ggctacttaa aggcccagga caaggacgag ctggcgcccg gcctcgccgt tccgaaggag
     6661 acgcccccgg tgtttctcgt ccacggcggc gacgacatca tcagccctcc cgaaggcacc
     6721 gtgatcatgt atctggccct caagcgggcc ggcattcccg ccgagctgca catctatgcc
     6781 cgcaccgccc acgacttcgg cgtccgccag gtcgaccggc cgtatgcgag ctggaccgac
     6841 acgtgtgcgc ggtggctgaa ggaccagggc ctcctcaaga aagaatgacc gaacaaaccg
     6901 ttcgagcggc tgccgtaaac gatcgccgtg gatataaaga aaactgccat tagcacggat
     6961 tccttcgcgc cccttctgcg ccgtggcctc gccacaactt cctggcggct aaggatttcc
     7021 ggcagcctgc cggcggtttc gagacattag cgttagaaga cttagaagac ccgccgggag
     7081 tttcccttat attttgcggc cgagcggaac ggcggcgatt ctgccacaag agccaacgac
     7141 caaattgtca gagagcacag cggaccccgc ggcgggcggc tagattggca gcctgcggca
     7201 ccaaggggaa ctatactgca aatagcgtac caatgttcac aaaaccggtc cgcccgcgtg
     7261 gcgatgctgg cgtgccgccc ggtgcgcttc gaagggcgag ccgcgccctc cctagttggc
     7321 cttcggccga ttgggatcga ccgttagcgg cacatccagc atatcgccat tgggcttgaa
     7381 gccgccgcca tcgacatcga cgaagatcgg attggcgacc gccacgggcg ggaacttgcc
     7441 gtaggtcggg cccatcacgg gcccaagctc cagtccctcg ccgattgcag ccacgatcag
     7501 gtgcgtgtcg gccttcacct cgaccggaat cttggcgtcg aacctcacgg tcttgtcgct
     7561 gaaccgatcg ggcgccagcc ggcgggtgaa attcagtgcg ggcagcggcc ggccgttggc
     7621 aaagacctgc acgcggttga tgtcgaacca gttggggcac tgcacgcgga cgtgcagcgt
     7681 cacgcggccg ccggtcgcgg gcagatccat ccccggcatc gcgccgactt ccttgcccag
     7741 cgagtcgccg acggcatcga ccgtcaaaaa cggcccggtg ctgacgacga cgtgtcccgc
     7801 ttcggcgttg tgcaccatct ccatcacgcc gatccgggcc ggatcgtcgg tcgagctgtg
     7861 gatgaagttc cggaagaacc ccgactcgtg atagttgtag tgcgagtcgg tgttgatcac
     7921 gcccgggtgg cggtagccca ggttgagcat ctgcatccag tgacaggtcg gattgcgctc
     7981 cagcttgccg tcggcctgtt tctggggcgg cgtgaagatg ccttgcggcg gatgaatctc
     8041 gatcacatcg acaaagccga acat
//
LOCUS       DASRJB010000512        10814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4092071, whole genome shotgun sequence.
ACCESSION   DASRJB010000512 DASRJB010000000
VERSION     DASRJB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4092071"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFB80_13410"
     CDS             <1..789
                     /locus_tag="VFB80_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34818.1"
                     /translation="VAGAEESLAKAAASRPALDRLKQLESAELTAGHQLADAKFAVRS
                     LDDKIATAKACLDYSAVAKTDPVKAAAAWTALVERWTIACQIAPLKPLTPEQLAASSL
                     QAAGMLQGQEASAIATIEKTPPEPLKTATDAEKPRMKARYVELRLIDQLDGVVAEFVR
                     QYGGLPGQDFQATVNQALFFGNGTTVDGWLKPAGENLVARLVKVEDAAQLADEMYVAV
                     FSRQATESEKQQVAAYLKDRTDKPVALGEMAWALLSSTEFRFNH"
     gene            856..2136
                     /locus_tag="VFB80_13415"
     CDS             856..2136
                     /locus_tag="VFB80_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34819.1"
                     /translation="MKCNYACQSAEHVLSRRSFVGGLAAGAAGLSASLAAAPAVAEQI
                     KAKQKRVLNIFLHGGVSQLETWDPKPNTDTGGPFRAIPTSVPGTHICELLPHTAQQLH
                     RLAIVRSLNTKNQDHGKGQVEMTTGRNRMLGTDYPHLGAVAAKSLLPEGKFPLPGHIL
                     IRGGGSGGHQAAYLGPKYASVVMDDGKPPQFTERPAALSAEADARRNAFRAVASDRFA
                     GRRRTADTDAYTFSYDQAADLLAQREVFDATKEPAADQERYGTSEFGRHCLLARRLLE
                     HNVPFVQINHSNYDTHFENFDFHIEQLGEFDRPFATLIADLADRGLLDDTLVCVMSEF
                     GRTPKINARYGRDHWGTAWSVLLGGAGIQPGACIGKTNANGTAVTEREVDHGHVFHTL
                     LQAVGVNSKSEFDIAGRKFPIADPAKGPIKELLV"
     gene            2229..3251
                     /locus_tag="VFB80_13420"
     CDS             2229..3251
                     /locus_tag="VFB80_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34820.1"
                     /translation="MPDAPKIDAAKTHLVLEFKHSRPLIACRFDPQSRYVYFGAEDNL
                     VHRFEIETKKLTPLAAHDSWVRALGTSPSGDVLFSGGYDGRLVWWPAGDAEPKPVRVV
                     DAHQGWIRALAVSPDGQLLATCGNDNLVKLWDAADGKVIGTLAGHAAHVYNVAFHPSG
                     QTLASCDLKGGLKEWDVGAGTLKRDLAAAAALYKYDNTFRADIGGARSMAYRTDGAQL
                     AVGGITNVSNAFAGIGHAAIVLIDLAAEGKPEGKVALQLEAKEKPNGVAWGLAHHPDG
                     FWIGLTGGGGGGWLYFWKGDAASEFHKLKLPSDGRGLSLSPQADRVAVAHADGNLRIF
                     ALHEKA"
     gene            3609..4541
                     /gene="ispH"
                     /locus_tag="VFB80_13425"
                     /gene_synonym="lytB"
     CDS             3609..4541
                     /gene="ispH"
                     /locus_tag="VFB80_13425"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZN34821.1"
                     /translation="MKVLLAAPRGFCAGVNMAIESLDLAIRAFGTPIYVYHEIVHNKY
                     VVDTFRSKGAVFIDDLGEVPVGATLLFSAHGVSPEIRRVARERKLRAIDATCPLVTKV
                     HLEAIRFAKEGYTILLIGHEGHDEVIGTMGEAPEAIVLVESEEHAGRLEFPPGARLAY
                     LTQTTLSVDDANRIINRLKERFPQIVGPPKEDICYATQNRQEAIHALAADADLVLVLG
                     SQNSSNSQRLKELAQERGVAAKLIDGAADIDERWFAGVENVVITAGASAPESVVQECV
                     AFLRDRYGATVESRTLRNEDVHFPLPRELRRIEA"
     gene            complement(4573..4998)
                     /locus_tag="VFB80_13430"
     CDS             complement(4573..4998)
                     /locus_tag="VFB80_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN34822.1"
                     /translation="MLVRDILSGKGRTVHTCTPDDSLADVVDLLVGHNIGSLIVMKDD
                     AMVGIITERDILRATAVTRGPLEFVKVHDRMTRLPVVISPHDAVADAMCVMTERRIRH
                     LPVVEQGRLAGIISIGDIVKAQHDELSRENHYLKSYIQS"
     gene            5328..6758
                     /gene="leuC"
                     /locus_tag="VFB80_13435"
     CDS             5328..6758
                     /gene="leuC"
                     /locus_tag="VFB80_13435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZN34823.1"
                     /translation="MTAAVKSQPRTMFQKIWDSHVVHAEPGKQTILYIDLQLVHEVTS
                     PQAFEGLRLSGRKVRRPELTVATPDHNIPTTDRSLPIADPISRQQVETLRANCRQFGI
                     KLYDMNDVRQGIVHVIGPELGLTRPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVL
                     ATQTLLQQLPKTLELRVDGPLPRGVTAKDLILYLIGQITTDGGTGYVIEYTGEAVRGL
                     NMEERMTVCNMSIEAGARAGMIAPDEKTYEFIRGREFAPQGAAFDAAVARWKELPTDA
                     GAKYDKSLSFQSADISPQVTWGTNPGQVAPVTAKVPDPASLGDETTRKSAAQALQYMG
                     LTAGTPLTELKIDRVFIGSCTNARIGDLRAAAAVVQGHKVAGSVHAMVVPGSGQVKLQ
                     AEKEGLDRIFTEAGFEWREAGCSMCLAMNPDKLEPGERCASTSNRNFEGRQGKGGRTH
                     LVSPAMAAAAAVAGHFVDVREWKYRA"
     gene            6805..7401
                     /gene="leuD"
                     /locus_tag="VFB80_13440"
     CDS             6805..7401
                     /gene="leuD"
                     /locus_tag="VFB80_13440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZN34824.1"
                     /translation="MTPFTTHTGLVATMDRANVDTDQIIPKQFLKRIERTGYGPFLFF
                     DWRYQDDGQTPNPKFELNQPKAQGASILLARRNFGCGSSREHAVWAIENYGFKAVIAP
                     SFADIFYNNCFKNGVLPIKLSEEQVESLFQKSAGGGYRLTVDLNSCQLSDSQGLKLSF
                     AVDESRRHCLLNGLDDIALTLQHADKIAAFERSRGIAV"
     gene            7584..8591
                     /locus_tag="VFB80_13445"
     CDS             7584..8591
                     /locus_tag="VFB80_13445"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN34825.1"
                     /translation="MRRCCLLLTLLIAAATAPINAVADELPKLPLGLKPVPVPTDNPQ
                     TPEKVALGKQLYFDPRLSCDDTTSCASCHDPKKGWSNSANFATGVRGQKGGRSAPTII
                     NAAYSDLQFWDGRAFRLEGQALGPIQNPIEMDHKLEDCVAKLNKVPGYRSQFQKIFGT
                     DVTPESVAKAISAFERTVLSGNAPYDRFKAGDANALSPSAQRGMKVFFGKGHCSACHT
                     GANFTDQAFHNVGVNINVRKPDLGRFEVTKVVGDKGSFKTPTLREIARTAPYMHDGSM
                     KALEEVVDHYIKGGTPNDQLDEEIFPIKMTPEEKADLVIFLKEGLSSPDYPDIPPPQL
                     P"
     gene            8649..9665
                     /locus_tag="VFB80_13450"
     CDS             8649..9665
                     /locus_tag="VFB80_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34826.1"
                     /translation="MNRTLSGLAFAALISLGLAAAAQDVKVETVVGGLSNPCGVAVQP
                     GTGAVVVSDSANGRVCRVDGGKLVDLITGFPKDVFGKGPFYDIGPLGVAFSGDGKSLI
                     VGDGGFKDGEECVRVYTMPEAGKPALNFDKDATAKLGPLAPAEGVLGEGNLYAIAVTP
                     TALYVTCNGDDTKGWVARATIEGTKYGKLERYLPTKEMVEVDAPVGITINKQGDIVVG
                     QFGENNKPKDSLLSFYSAKTGKLLKNDETGLYDITALAYSPKTGLLYATDFAWMKTDE
                     GGLFRIDAAQTGVKATKIVGLDKPTALAFAPDGTLYITVIGPKGAEENAPKDGKLLKI
                     APGL"
     gene            complement(9666..10553)
                     /locus_tag="VFB80_13455"
     CDS             complement(9666..10553)
                     /locus_tag="VFB80_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34827.1"
                     /translation="MQIFTRQVLTAFVLLAIGPARLWAAGEEKLGNEPLPDGNYAKWP
                     GLAAVVNDKSRVYYQWVNGNENFYYIGSTAELSAILARFAAATLKSHEVLITPGPGKA
                     WSFNRENEFTVNCHLQIFDGIAGHQLTRDQGDQVWPKEPRLTIFTGGDLDLAKLEIPK
                     GLTFITPGELSARIRKGSASKDKSVKGWSAGVLAHHDPFDPANLAVIQKLLADEDDWV
                     RLNAAGVAPLFGPQAKAALPALKDCLARKDEGLKTAAQKAIEQIEAAPDRSAAERERA
                     AAIATIDDFVANYRKAAAK"
BASE COUNT         2021 a         3613 c         3455 g         1725 t
ORIGIN      
        1 gtcgccgggg ccgaagagtc gctcgcaaaa gccgccgcat cgcgaccggc gctcgatcgg
       61 ctgaagcagc tcgaaagcgc cgagctgacc gccggccacc agctggcgga cgcgaagttt
      121 gcggtccgtt cgctggacga caagatcgca accgccaagg cctgcctcga ctactccgcc
      181 gtagcgaaga ccgatccggt caaggcggcg gcggcctgga ccgcgctcgt cgaacgctgg
      241 acgatcgcct gccagatcgc cccgctcaag ccgctgacgc cggagcaact ggcggccagc
      301 agcctgcaag ctgccggaat gctccagggc caggaggcca gcgctatcgc tacgatcgag
      361 aaaacgccgc ccgaaccgct caagacagcc accgacgccg agaagccgcg gatgaaggcc
      421 cgctacgtcg agctgcggct gatcgatcag ctcgacggcg tcgtggccga gttcgtgcgg
      481 caatacggcg gcctgccggg ccaggatttc caggccacgg tgaaccaggc gctgtttttc
      541 ggcaacggca cgaccgtcga tggctggctg aagcccgcgg gcgagaacct cgtcgcccgg
      601 ctggtcaaag ttgaagatgc tgcacagctc gccgacgaga tgtatgttgc tgtattctcg
      661 cgccaagcca ccgagagcga gaagcagcaa gttgccgcgt acctgaaaga ccgcaccgac
      721 aagcccgtgg ccctcggcga gatggcttgg gccttgcttt cgtccacgga gttccggttc
      781 aatcactaac gtagacgtct cgctccgcga gacgcggcct cacgcggagc gtgaggacta
      841 ctatggagcc gccccatgaa atgcaactac gcctgtcagt cggctgagca cgtgctgtcg
      901 cggcggtcct ttgtgggcgg cctggctgcg ggcgcggcgg gcctgtcggc gtcgctcgct
      961 gccgcgccgg cggtggccga gcagatcaag gcgaagcaga agcgggtgct caacatcttc
     1021 ctgcacggcg gcgtgagcca gctggagacg tgggatccga agcccaacac cgacaccggc
     1081 gggcccttcc gcgccatccc gacgtcggtg ccgggcaccc acatttgcga gctgctgccg
     1141 cacaccgccc agcagctgca ccgcctggcg atcgtccgca gcctgaacac gaagaaccag
     1201 gaccacggca agggccaggt cgagatgacg accggccgca accggatgct gggcacggac
     1261 tatccgcact tgggcgcggt cgccgcgaaa agtttgctgc cggagggcaa gttcccgctg
     1321 ccggggcaca tcctcatccg cggcggcggc tcgggcggcc accaggcggc ctacctgggg
     1381 ccgaagtatg ccagcgtggt gatggacgac ggtaagccgc cgcaattcac cgagcggccg
     1441 gccgccctct ccgccgaggc cgatgcccgt cgcaacgcct tccgcgcagt ggccagcgac
     1501 cgctttgccg gccgccgccg cacggccgac accgatgctt acacgttctc gtacgaccag
     1561 gcggccgacc tgctcgccca gcgcgaggtg ttcgatgcca ccaaggagcc ggcggccgac
     1621 caggagcggt acggcacgag cgagttcggc cggcactgcc tgctggcccg ccgactgctg
     1681 gagcacaacg tgccgttcgt gcagatcaac cactcgaact acgacacgca tttcgagaat
     1741 ttcgatttcc atatcgagca gctcggcgaa ttcgaccggc cgttcgcaac gctgatcgcc
     1801 gacctggccg accgcggcct cttggatgac acgctggtct gcgtgatgtc ggagttcggc
     1861 cgcacgccca agatcaacgc ccgctacggc cgcgaccact ggggcacggc ctggagcgtg
     1921 ctgctcggag gcgcgggcat ccagccgggc gcctgcatcg gcaaaaccaa cgccaacggc
     1981 accgccgtca ccgagcgcga agtcgatcac ggccacgtgt tccacacgct gctgcaggcc
     2041 gtcggcgtca attccaagag cgaattcgac atcgccggcc gtaagttccc catcgccgac
     2101 ccggccaagg gaccgatcaa ggagctgcta gtgtaggcga gcgggaggcg tgagcctccc
     2161 gatttggtcg cgttcgaagc cgccggatta tcgggaggct gacgcctccc gctcgccagg
     2221 actacatcat gcccgacgca cccaaaatcg acgccgccaa gacgcacctg gtgctggaat
     2281 tcaagcacag ccggccgctg atcgcctgcc ggttcgatcc acaatcgcgg tacgtctatt
     2341 tcggcgcgga agacaacctg gttcaccgct tcgagatcga gacgaagaag ctcacgccgc
     2401 tggccgcgca cgacagctgg gtgcgggcgc tgggaacatc gcccagcggc gacgtgctct
     2461 ttagcggagg ctacgatggc cggctggtct ggtggccggc cggggacgcc gagcccaagc
     2521 ccgtccgagt ggtggacgcc catcaaggct ggatccgcgc gctcgcggtc agccccgatg
     2581 gccagctcct cgccacctgc ggcaacgaca acctcgtcaa gctctgggat gcggcggacg
     2641 gcaaagtgat tggcacgctc gccggccacg cggcgcacgt ctacaacgtg gcctttcatc
     2701 ccagcggcca gacgctcgcc agctgcgacc tcaagggcgg cctcaaggaa tgggacgtcg
     2761 gggcgggtac gctcaagcgc gacttggcgg cggctgccgc gctctataag tacgacaaca
     2821 cctttcgggc cgacatcggc ggcgcgcgga gcatggccta tcgcaccgac ggcgctcagc
     2881 tggccgtcgg cggcatcacc aacgtcagca acgccttcgc cggcatcggc catgcggcaa
     2941 ttgtgctgat cgacctggcc gcggagggaa agcccgaggg caaagtcgcc ctccagctcg
     3001 aggccaagga gaagcccaac ggcgtcgcct ggggcctggc ccatcatccg gacggcttct
     3061 ggatcggcct gaccggcggc ggcggtggcg gctggctcta tttctggaag ggcgatgccg
     3121 ctagcgagtt ccacaagctc aagctgccca gcgacggccg cggcctgtcc ctgtcgccgc
     3181 aggctgatcg cgtggctgtc gcccacgccg acggcaacct gcggatcttt gccctgcacg
     3241 agaaggcgta gcgcgccgct tccggcaagc ggatgtgaac attggtacgt tatttgcagt
     3301 gatgttccac ttgcgcggcg agtccgccat tttggcgctg gccgacttcg gtttcctgcg
     3361 aacgacggcg gcgtcaaacg cttataacaa ctacttaccg ggtttcggcc catgtggcgc
     3421 aaacccaagt cccgtctcag cttgcggcgg gaatttcgac gagagggcat gtcgcggtag
     3481 ctcaaccggg tggtgcatga gcgaggaggc cgggccccgc cggtgaaagt cgccgaaagt
     3541 ctggcggttg aagaatgggc gggtgaccgc aaggcggatc gagagcaatt tgggctaaaa
     3601 tcaagagcat gaaagtcctg ctggccgcgc cccgcgggtt ttgcgcgggc gtgaacatgg
     3661 cgatcgagag cctggacctg gcgatccggg cgttcggtac tccgatctac gtttatcacg
     3721 agatcgtcca caacaagtac gtcgtcgata cgttccggtc caaaggggcg gtcttcatcg
     3781 acgacttggg cgaagtgccc gtgggggcga cgctgctctt ctcggcccac ggcgtctcgc
     3841 ccgagattcg ccgcgtggcc cgcgagcgga agctgcgggc gatcgacgcc acctgcccgc
     3901 tggtgaccaa ggtccacttg gaggcgatcc gcttcgccaa ggaaggctac acgatcctgc
     3961 tcatcggcca cgaagggcac gacgaggtga tcggcacgat gggcgaagca cccgaggcga
     4021 tcgtgctcgt cgaatcggaa gagcacgccg gccggctgga gttcccgcct ggcgccaggc
     4081 tggcttacct cacccagacg accctctcgg tcgatgacgc caaccggatc atcaaccgat
     4141 taaaggagcg gtttccgcag atcgtcggcc cgccgaagga agacatctgc tacgccaccc
     4201 aaaaccgcca ggaggcgatc cacgccctgg ctgccgacgc cgacctggtg ctggtcctgg
     4261 gcagccagaa cagctccaac agccagcgct tgaaggaact cgcccaggag cgcggcgtcg
     4321 cggccaaact gatcgacggc gcggccgaca tcgacgagcg gtggttcgcc ggcgtcgaga
     4381 acgtcgtgat cacggccggc gcgagcgccc cggagtcggt ggtccaggag tgcgtggcct
     4441 ttctccgcga ccgttacggc gcgacggtag agagccgcac gctccgcaac gaagacgtac
     4501 actttccgct gccgcgggag ctgcggcgga tcgaggccta gtgcgtccgg cgtggacgcc
     4561 ggggctaagc ggctacgact ggatgtagct cttcaaataa tggttctcgc gcgacagttc
     4621 gtcgtgctgg gccttgacga tgtcgccgat cgagatgatg cccgccagcc ggccctgctc
     4681 gaccaccggc aagtgccgga tccgccgctc ggtcatgacg cacatcgcgt cggcgaccgc
     4741 gtcgtgcggc gagatcacca ccgggagccg cgtcatccgg tcgtgaacct tgacgaattc
     4801 cagcggcccg cgggtcacgg cggtcgcccg caggatgtcg cgctcggtga tgatgccgac
     4861 cattgcgtcg tccttcatca cgatcagcga accgatgttg tgccccacga gcagatcgac
     4921 cacgtcggcc agcgagtcgt ccggggtgca ggtatgcacc gtccggcctt tgccggaaag
     4981 aatatctcgc accagcatga acccaagcct ccgcctgcaa atcgggatga gagaggaata
     5041 ccgaacaggg gcgctcgtct ctgtgctgct taccgcgtga aaacgcggta agcgaaaaac
     5101 tcagcagctt gcggacatcc taaagtcgcc gcgcaagcgc cgtcaaacat tttctccatt
     5161 gcttgtgaaa agcgtcacaa actccaggca ccgcgaaccc cgcaggccag attcggctcc
     5221 catccccagc cgcggcggtg caaggtagaa ttgaacaaat tcttgccgcg gctgcgtccc
     5281 gcccagatta gccgcgaaaa attgctctcc agcccgctgt tccaagtatg accgctgctg
     5341 tgaaatccca gcccaggacc atgttccaga aaatctggga cagccacgtc gtccatgccg
     5401 aaccgggcaa gcaaacgatc ctgtacatcg atttgcagct cgtgcacgaa gtgaccagcc
     5461 cgcaggcctt cgaaggcctg cggctttccg ggcgaaaggt gcggcggccg gagctgacgg
     5521 tcgcgacgcc ggaccacaac attcccacga ccgaccgctc gctgccgatc gccgacccga
     5581 tctcgcggca gcaggtcgag acgctgcggg ccaactgccg gcagttcggc atcaagctct
     5641 acgacatgaa cgacgtgcgg caggggatcg tgcacgtcat cgggcccgag ctgggcctga
     5701 cgcggccggg catgacgatc gtctgcggcg acagccacac ggccacgcac ggggcgttcg
     5761 gcgcactggc gttcggcatc ggcacgagcg aagtcgagca cgtgctggcg acgcagacgc
     5821 tcctgcagca gctgcccaag acgctggagc tgcgggtcga tgggccgctg ccccgcggcg
     5881 tcacggccaa ggacctgatc ctgtacctga tcggccagat cacaaccgac ggcggtaccg
     5941 gctacgtcat cgagtacacg ggcgaggccg tccgcggcct gaacatggaa gagcggatga
     6001 ccgtctgcaa catgtcgatt gaagccggcg cgcgggctgg catgatcgcg cccgacgaga
     6061 agacgtacga gttcatccgc ggacgcgagt ttgcaccgca gggcgccgcg ttcgacgccg
     6121 cggtggctcg ctggaaggaa ctgccaacgg atgccggcgc gaagtacgac aagtcgctgt
     6181 cgttccagtc cgccgacatc tcgccgcaag tcacctgggg cacgaatccc ggccaggttg
     6241 cacccgtgac cgcgaaagtg cccgatccgg cctcgctcgg cgacgaaacg acacggaaat
     6301 cagccgcaca ggctctgcaa tacatgggtc taacggccgg cacgccactg accgagctga
     6361 agatcgaccg cgtcttcatc ggctcctgca ccaacgcccg catcggcgac ctgcgagccg
     6421 cggccgccgt cgtgcaggga cacaaggtcg cgggcagcgt ccacgcgatg gtcgttcccg
     6481 gcagcgggca ggtaaaactg caagccgaga aggaaggcct cgaccgcatc ttcaccgagg
     6541 ccggcttcga atggcgcgaa gccggctgct cgatgtgcct ggcgatgaac cccgacaagc
     6601 tcgagcccgg cgagcgctgc gccagcacca gcaaccgcaa cttcgaaggc cgtcaaggca
     6661 aaggcggccg tacgcacctc gtctcccccg ccatggccgc cgccgccgca gtggcgggcc
     6721 attttgtgga tgtgcgggaa tggaagtatc gggcgtaagt gcgctccgca caactttgaa
     6781 ctttgaactc ggaaatttga actgatgact ccctttacca cccacaccgg cctggtcgcc
     6841 acgatggacc gggcgaacgt cgataccgac cagatcatcc ccaagcagtt ccttaagcgg
     6901 atcgagcgga cggggtacgg gccgttcctg ttcttcgatt ggcggtacca ggacgacggc
     6961 cagacgccga atccgaagtt cgagctgaac cagcccaagg cccaaggtgc gtcgatcctg
     7021 ctggcccggc gcaacttcgg ctgcggcagc agccgcgagc acgccgtgtg ggcgatcgag
     7081 aactacggct tcaaagccgt catcgccccg agcttcgccg acatcttcta caacaattgc
     7141 ttcaagaacg gcgtgctgcc gatcaagctc agcgaagagc aggtcgagag cctgttccag
     7201 aagtcggccg gcggcggcta ccgactcacg gtcgatctga attcctgcca gctcagcgat
     7261 tcgcagggcc tgaagctgag cttcgccgtc gatgaatcgc ggcggcattg cctgctgaat
     7321 ggcttggacg acattgccct gacgctccag cacgccgaca agatcgcggc cttcgaacgc
     7381 agccgcggaa tcgcggttta ggcgagcggg aagcgttagc ttcccgatga tttccccttc
     7441 tccatgctat cgggaggctt acgccttccg ctcgccgggc tggctatagc cacttgctcg
     7501 ccccctgccg gtcggcaaga atactgcgat tcgagcccgc ccattccccc cgcccgccgc
     7561 tcctcgcgcg agacgaccct gacatgcgcc gctgttgtct tttgctcacc ttgctgattg
     7621 ccgccgccac cgccccaatc aatgcagtcg ccgacgaact gcccaagctg ccgctggggc
     7681 tcaagccggt tccggtaccg accgataacc cgcagacgcc cgaaaaggtc gccctgggca
     7741 agcagctcta tttcgaccca cggctctcct gcgacgacac caccagctgc gcgagctgcc
     7801 acgacccgaa gaagggctgg tcgaacagcg cgaatttcgc gacgggcgtt cgcggacaga
     7861 agggtggtcg cagtgctccg acgattatta acgcggccta cagcgacttg cagttctggg
     7921 acggccgggc gtttcgcctg gaaggccagg ccctggggcc gatccagaac ccgatcgaga
     7981 tggaccacaa gctcgaggac tgcgtcgcca agctgaacaa ggttcccggc taccgctccc
     8041 agttccagaa gatttttggc accgacgtca cacccgaaag cgtggcgaag gcgatttcag
     8101 cgttcgagcg aacggtactc tccggcaacg caccctacga ccggttcaag gcgggcgacg
     8161 ccaatgccct ctcgccctcc gcccagcgcg gcatgaaggt cttcttcggt aaggggcatt
     8221 gctcggcctg ccacaccggc gcgaatttca ccgatcaggc gttccacaac gtcggcgtga
     8281 atatcaacgt gcgcaagccc gatctgggcc gctttgaagt gaccaaggtc gttggcgaca
     8341 agggctcgtt caagacgccc acgctccgcg agatcgcccg cacggcgccc tacatgcacg
     8401 acggcagcat gaaggcgctg gaggaagtcg tggaccacta catcaagggc ggcactccca
     8461 acgaccagct cgacgaagag atcttcccga tcaagatgac cccggaggag aaggccgacc
     8521 tcgtgatctt cctgaaggaa ggcctttcca gcccggacta tcccgacatc cctccgcccc
     8581 agctgccctg acttccggcg gctgcggcac gacttgcacc ctcgcatcac catccaggag
     8641 cacggaatat gaatcgcacg ctgtccggtt tggccttcgc ggccctcatc agcctgggcc
     8701 tcgcggccgc ggctcaagac gtcaaggtcg aaaccgtcgt gggcggcctg tcaaatccgt
     8761 gcggcgtcgc cgtgcagccg ggaacggggg cagtcgtcgt ctccgacagc gccaatggcc
     8821 gcgtctgccg cgtggacggc ggcaagctgg tggacctgat caccggcttc ccgaaggacg
     8881 tgttcggcaa ggggccgttc tacgacatcg gcccgctggg cgtggccttc agcggcgacg
     8941 gcaagtcact gatcgtcggc gacggcggct tcaaggacgg cgaggaatgt gtccgcgtct
     9001 atacgatgcc cgaggccggc aagccggccc tgaacttcga caaggacgcc actgccaagc
     9061 tcggcccgct ggccccggcc gaaggcgtct tgggcgaagg caacctgtac gcgatcgccg
     9121 tcacgccgac cgcgctctac gtcacctgca acggcgacga caccaagggc tgggttgccc
     9181 gggccacgat cgaaggcacg aagtacggca agctggagcg ctacctgccg accaaggaaa
     9241 tggtcgaagt cgatgccccc gtcggcatca cgatcaacaa gcagggcgac atcgtcgtcg
     9301 gccaattcgg cgaaaacaac aagcccaagg acagcctgct cagcttctac agcgccaaga
     9361 ccggcaagct gctgaagaac gacgagacgg gcctgtacga catcacggcg ctggcctact
     9421 cgccgaagac ggggttgctg tatgccaccg acttcgcctg gatgaagacc gatgaaggcg
     9481 gcctgttccg catcgatgcg gcccagaccg gcgtgaaggc gaccaagatc gtcggcctcg
     9541 acaagccgac ggccctggcc ttcgcgcccg acgggacgct ctacatcacc gtgatcggac
     9601 ccaagggggc cgaggaaaac gcccccaagg acggcaagct gctgaagatt gctccagggc
     9661 tatagtcact tcgcggctgc ctttcggtaa ttcgccacga agtcgtcgat cgtcgcaatc
     9721 gccgccgcgc gctcgcgctc ggcggccgag cgatcggggg cggcttcgat ttgctcgatc
     9781 gccttttggg cggcggtttt gaggccctcg tcctttcgcg ccaggcagtc cttcagcgcc
     9841 ggcagcgcgg ccttggcctg cgggccgaag aggggcgcca cgcccgccgc gttcaggcga
     9901 acccaatcgt cctcgtcggc cagtagcttt tgaatgacgg ccaggttagc cggatcgaac
     9961 gggtcgtgat gcgcgagcac accggcggac cagcccttca ccgatttgtc cttgctggcc
    10021 gagcccttgc ggatgcgggc gctcagttcg cccggcgtga tgaatgtcag tcccttgggg
    10081 atttccagct ttgccaggtc gagatcaccc cccgtgaaga tcgtcagccg cggctccttg
    10141 ggccaaacct ggtcgccctg gtcgcgggtg agctgatggc cggcgatgcc gtcaaagatt
    10201 tgcaggtggc agttgaccgt gaattcgttc tcacgattga acgaccaggc cttgccgggt
    10261 cccggcgtaa taagcacctc gtgcgacttc aacgtggcgg ccgcaaagcg ggccaggatg
    10321 gcgctcagct cggccgtgct gccgatgtag tagaagttct cgttgccgtt gacccattgg
    10381 taatagacgc ggctcttgtc gttgacgacc gccgcgaggc ctggccattt cgcatagttg
    10441 ccgtcgggga gcggctcgtt gccgagcttc tcctcgcccg ccgcccacag ccgggcgggc
    10501 ccgatggcga gcaggacgaa tgccgtaagt acttggcgcg taaagatttg catgactgtg
    10561 actccgtagc aggcggcttc cagtcgccgc caatgcgatc gaacaaacga cgaccgcaat
    10621 aaagacgatc tgtctgccgc gaacgatttc acggcagcgg cgatccagcc gccgctactt
    10681 cacgacgcgg ccttgcggcg attcgaggcc ggcggcctgg cggagcatgt tcaggacgtg
    10741 ctcgaccttc ttgtgggtct gggcggtctg cgtcacgacg atcaggccgt gatactcgct
    10801 gatcgagccc gcgc
//
LOCUS       DASRJB010000513         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3243920, whole genome shotgun sequence.
ACCESSION   DASRJB010000513 DASRJB010000000
VERSION     DASRJB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243920"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1470
                     /gene="carA"
                     /locus_tag="VFB80_13460"
     CDS             328..1470
                     /gene="carA"
                     /locus_tag="VFB80_13460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZN34828.1"
                     /translation="MTKPAKLALEDGTVFTGTSFGAAGEVDGEVCFNTSMTGYQEILT
                     DPSYRGQIVTMTYTEMGNYGVNAEDVESGKPHLAGFIVKEYSERPSNFRAQESLDAYL
                     KRHGIVGLAGIDTRALVRRLRTGGAMKGVVSSVDLDDASLVRKAKASPGLVGRDLVRE
                     VIPAQARQWEGNLSTWAKLPGQPNPAFEQPDFHVVALDYGMKWNIARHLRDEGCRITI
                     LPGTASAAEVLSHQPDGVFLSNGPGDPEPLHYAQECIRGVLGKVPVFGICLGHQLLGL
                     ACGAKTFKLKFGHRGANQPVLNVLSGKVEITAQNHGFAVDEKSMPGFLEITHRNLNDN
                     TVAGLRHKEVAAFSVQYHPESSAGPHDSRYLFRQFREQMEAERAGA"
     gene            complement(1471..2232)
                     /locus_tag="VFB80_13465"
     CDS             complement(1471..2232)
                     /locus_tag="VFB80_13465"
                     /inference="COORDINATES: protein motif:HMM:NF040416.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34829.1"
                     /translation="MRLEQAVFTSTRSSRLDGYQLAARSSGVSEALAKELTTWGPAHD
                     SLWDTRLDARTVNFHPLSTGDYCLSWTSIAGAEYSGRGGGRIYTQMFLLPRTALTRFA
                     CDPFLVLRALSAAGRMVVHDQVPDSLPSVPLLGRCERADAGIATQVIDEVGAKVFDDL
                     VDAVANAPKVAVLTNGHVERLFQAVLHTFSPDDRLTISFTTGLKDSPRRPFKLFVLPN
                     DQSIVRQSQRLNGARVVDLVGEDVRGSSRLSGAAK"
     gene            complement(2281..3186)
                     /locus_tag="VFB80_13470"
     CDS             complement(2281..3186)
                     /locus_tag="VFB80_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34830.1"
                     /translation="MRANGLLEMDSYRLAAYTVPCPCYICGGGNNFDAELCRHCHAPM
                     ALAHQATSQKIHPRMIAAVGPSGAGKTVYLGMLTDMLSRQDENMQLLARGAFSIRLQQ
                     NAMASLGRCEFPHKTPNEPDRWNWVHCQVLRKGQRATELIMPDLAGEALLEEVDHPNS
                     YPVIRQFLSKCSGVMVLVDAPRVESGDHDEDFHTMKLITYLSELSADKKTNWHTRPVA
                     LVFSKADQCEHCFDDPQAFARRHTPGLVQQCRERLKRHHFFAVGVAGACGFRSEPFGK
                     VHVPLRIEPRGIVEPFKWLLDELAT"
     gene            complement(3183..4451)
                     /locus_tag="VFB80_13475"
     CDS             complement(3183..4451)
                     /locus_tag="VFB80_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZN34831.1"
                     /translation="MRRFLPKTLNQWLLAAIPLVLLLAWLLNHWLLGVAAVLSALCVL
                     AVRNPKFEGNWRRLPWLQKLFPQPAAPAPIQPAPVMPAPPPPPRRIRRHEAGASLAEQ
                     MIDQGRVALLLRPQVATSLSAADLEQAQAALDEAMAIVPQGQVAMRSRAFDHADDENP
                     VRAERLIQVEGFFLDRFCVTNHDYEQFVADGGYEQMSLWDESIWPAVLGFVDRSGQPG
                     PRYWENSTYAANLADHPVVGVSWYEACAYARWAGKRLPTDPEWVKAGSWPVFAEGAKP
                     VQRRFPWGDSMDRKRVNVWGSGYVSTVAVHAMPAGASVNGVQQLIGNVWEWTSSTFGA
                     WEPASRKIETALPLKSVRGGAYDTYFDTQSHCQFQSGESPLARKHNIGFRCSLGFCDV
                     IHQIDQAAEDDADTRNTDRLPTATPEEVHA"
     gene            complement(4521..>4781)
                     /locus_tag="VFB80_13480"
     CDS             complement(4521..>4781)
                     /locus_tag="VFB80_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34832.1"
                     /translation="VNQQRRELVEQRADVTTELRLLRELVEQRGGTRGTSSYEPEEEP
                     ALVGTVSRSEASSGAPADPVVSSVMAQFARLQKDVAQRRKKK"
BASE COUNT          845 a         1554 c         1580 g          802 t
ORIGIN      
        1 tgccggagca gatggcgagg cagagcctcg gggaagttga atccgaggca gagcctggga
       61 cgcagggaac gccacagagg gcgttcccta aggcgcgacg ccacacgcag ccgcgcaaca
      121 aaaaaggagg tctcttgttc gccgcaacaa gaggcctccc gtatttggct gtttcggttt
      181 tgggttgccc caattccgaa gacccacagc cgtagtgtgg gtatcggaag cggcgcgcag
      241 ccggctttaa gacaccaacc gccgccgtac cattcggcgc aactttcacg aatcctccca
      301 ctgacaactg acgactgacc acggcccatg accaagccag caaagcttgc cctcgaagac
      361 ggcacggtat ttacgggaac ttcgttcggc gcggcgggcg aggtggacgg cgaagtctgc
      421 ttcaacacct cgatgaccgg ctatcaggaa atcctcaccg atccgagcta tcgcgggcag
      481 atcgtgacga tgacctacac cgagatgggc aactatggcg tcaacgccga ggacgtcgag
      541 agcggcaaac cccacctcgc cgggtttatc gtgaaggagt attccgagcg gccgagcaat
      601 ttccgcgcgc aggaatcgct cgacgcttat ctgaagcggc acggcatcgt cggcctggcg
      661 ggcatcgaca cgcgggccct cgtccgccgc ttgcggaccg gcggcgcgat gaaaggcgtc
      721 gtctccagcg tcgatctcga cgacgccagc ctcgtccgca aggccaaggc cagccccggc
      781 ctggtcggcc gcgacctggt gcgtgaagtc atccccgcac aagcacgcca gtgggaaggc
      841 aacctctcca cctgggccaa actgcccggc cagcccaatc cggcattcga gcagcccgac
      901 tttcacgtcg tcgcgctcga ctatggcatg aagtggaaca tcgcccggca cctccgcgac
      961 gaaggctgcc ggattacaat tttgccagga accgcatcgg ccgcagaagt gctatcgcac
     1021 cagccggacg gcgtcttcct cagcaacggc ccgggcgacc ccgagccgct gcattacgcc
     1081 caggagtgca ttcgcggcgt cttgggcaag gtgcccgtct tcggcatctg cctgggccat
     1141 caattgctcg gactggcctg cggcgcgaag accttcaagc tcaagtttgg ccaccgcggc
     1201 gcgaatcagc cggtgctgaa tgtgctgtcg ggcaaagtcg agatcaccgc ccagaatcac
     1261 ggcttcgccg tcgatgagaa atcgatgccg ggcttcttgg aaattaccca ccgcaacctg
     1321 aacgacaaca cggtcgccgg actgcggcac aaggaagtcg cggcgttcag cgtgcaatac
     1381 caccccgaat cttccgccgg accgcacgac agccgctatc tgttccgcca gttccgcgag
     1441 cagatggaag ccgagcgggc cggggcgtga ttacttcgcg gcgcccgaga gccggctgct
     1501 gccgcggacg tcttcgccca cgagatcgac aacgcgtgcg ccgttgagcc gctgcgactg
     1561 gcggacgatc gactggtcgt tgggcaggac gaagagtttg aacgggcggc ggggcgagtc
     1621 cttgaggccc gtggtgaacg aaattgtcag ccggtcgtcc gggctgaacg tgtgcaggac
     1681 ggcctggaac agccgctcga cgtggccgtt ggtcagcacg gcgaccttcg gtgcattcgc
     1741 gacggcatcg acgaggtcgt cgaaaacttt cgcgccgact tcgtcgatga cttgcgtagc
     1801 gatgcccgcg tcggcccgct cgcagcggcc caagagcgga acgctgggca gcgagtccgg
     1861 cacctggtcg tgaacgacca tccgtcccgc ggcggacaga gcgcgcaata ccaggaacgg
     1921 atcacaggca aatcgcgtca gggcggtgcg tggcaggagg aacatctgcg tatagatgcg
     1981 tccgccgccg cggccgctgt attcagcgcc ggcgatcgag gtccaggaaa ggcaatagtc
     2041 gccggtggag agcggatgga aattgaccgt gcgggcgtcg agccgcgtgt cccacagcga
     2101 gtcgtgagcc ggtccccagg tggtcaactc cttggccagg gcctcgctga cgccgctgct
     2161 gcgcgcggcc agttgatagc cgtccaaccg gctgctgcgc gtcgaagtga agaccgcttg
     2221 ttccaaacgc atggcacaca cccgcaatgc caaggccgga ggccgatcga ggttctggcc
     2281 ttacgtcgcc agctcgtcga gcagccactt gaacggttcg acgatgccgc ggggctcgat
     2341 ccgcagaggc acgtgcactt tgccgaacgg ctcgctgcgg aagccgcagg ctcccgccac
     2401 accgacggcg aagaagtggt gccgcttgag gcgctcgcgg cattgctgca cgagtcccgg
     2461 cgtgtgccgg cgggcgaagg cctgcgggtc gtcgaaacag tgttcgcatt ggtcggcctt
     2521 ggagaagacc agtgccactg gccgcgtgtg ccagttggtt ttcttgtcgg ccgaaagttc
     2581 gctgaggtag gtgatgagct tcatcgtgtg gaagtcttcg tcgtggtcgc ccgattcgac
     2641 ccggggcgca tcgacgagca ccatcacgcc cgagcacttg ctcaggaact ggcggatgac
     2701 cggataggag ttgggatgat cgacttcttc gagcaacgct tcgccggcca gatcgggcat
     2761 gatcagctcg gtggcccgct gtcccttgcg gaggacctga caatggaccc aattccagcg
     2821 gtcgggctcg ttgggcgtct tgtgcgggaa ctcgcagcgg cccagcgacg ccatggcatt
     2881 ctgctggagc cggatcgaga acgcgccgcg ggccaggagc tgcatgttct cgtcctgccg
     2941 cgagagcatg tcggtgagca tgcccaggta aaccgttttg cccgcgcccg acggtccgac
     3001 cgcggcgatc atccgcggat ggatcttctg cgaggtggcc tggtgggcca gggccatcgg
     3061 cgcgtggcag tgccggcaga gctcggcgtc gaagttgttg ccgccgccgc agatgtagca
     3121 cgggcagggc accgtgtagg cggcgaggcg gtaggaatcc atttccaaga ggccgttggc
     3181 tctcatgcgt gcacctcttc gggggtggcg gtgggcaggc ggtccgtgtt gcgggtgtcg
     3241 gcgtcgtcct cggcggcctg gtcgatttga tgaatcacgt cacagaaccc cagcgagcag
     3301 cggaagccga tgttgtgctt gcgagccagc gggctctcgc cgctttgaaa ttgacaatgc
     3361 gactgcgtgt caaagtatgt gtcgtacgcg ccgccgcgga cgctcttgag cggcaaggcc
     3421 gtctcgatct tgcgcgacgc gggctcccag gcgccaaatg tgctgctcgt ccattcccag
     3481 acgttgccga tcaactgctg gacaccgttg acgctcgcgc ccgccggcat ggcgtgcacg
     3541 gcgactgtgc tcacgtaacc gctgccccag acgttgacgc gtttgcggtc catcgaatcg
     3601 ccccaaggga accgccgctg cacgggcttg gccccttcgg caaagacggg ccacgagccg
     3661 gcctttaccc attcggggtc ggtgggcaat cgcttaccgg cccagcgggc ataggcgcag
     3721 gcctcatacc acgacacgcc gaccaccggg tgatcggcca gatttgccgc ataggtgctg
     3781 ttctcccaat accgcggccc aggctggccc gagcgatcga cgaagcccag cacggccggc
     3841 cagatcgact cgtcccacag cgacatctgc tcgtagccgc cgtcggcgac gaactgttcg
     3901 tagtcgtgat tcgtgacgca gaaccggtcg aggaagaagc cttcgacctg gatcagccgc
     3961 tcggcgcgga ccgggttttc gtcgtcggcg tgatcgaagg cccgcgaccg catggccacc
     4021 tggccctgcg ggacgatggc catcgcctcg tccagcgccg cttgagcctg ctccagatcg
     4081 gcggcggaaa gacttgtcgc cacctgcggc cggaggagca aagccacgcg gccctggtcg
     4141 atcatctgct cggccagcga tgcgccggcc tcgtgccgcc gaatgcggcg gggcggaggc
     4201 ggcggggctg gcataacggg ggccggctgg atcggcgccg gggccgctgg ttgcggaaac
     4261 agcttttgaa gccacggcag gcgccgccaa ttgccctcga atttcggatt gcgcaccgcc
     4321 agcacgcaga gcgcgctaag gacggccgct acgcccagca gccaatgatt gagcagccag
     4381 gccaggagca agaccagcgg gatggccgcc agcagccact ggttcagcgt cttgggaagg
     4441 aatcgccgca ttgctttcac cctcgtaggt acaccatcgc ccgttggcct cgcattccgc
     4501 tcgtccgcgc tctccgcggc ttacttcttc ttcctccgct gggccacatc cttttgcagc
     4561 cgggcgaact gagccatgac ggagctgacg accggatcgg ccggagcgcc gctactggct
     4621 tcgctgcggg agaccgtgcc caccagggcc ggctcttcct cgggctcgta cgaggacgtc
     4681 ccgcgcgtgc caccccgttg ctcgaccagc tcgcggagca accgcagctc ggtcgtgaca
     4741 tcggcccgct gctcgaccag ctcgcggcgc tgctggttga c
//
LOCUS       DASRJB010000514         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2107630, whole genome shotgun sequence.
ACCESSION   DASRJB010000514 DASRJB010000000
VERSION     DASRJB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107630"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(460..2883)
                     /gene="rnr"
                     /locus_tag="VFB80_13485"
     CDS             complement(460..2883)
                     /gene="rnr"
                     /locus_tag="VFB80_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZN34833.1"
                     /translation="MSFDPKNAVNEELTEEVVEKEENEEEAEAEKASPAARKLEPIVL
                     AHVLGPKYQPVKPRVIAKQLKLASEQHRALKLAIRRLVKAGKLVYGAGHQVRPPKPAA
                     GARSQEPEARGQGTGDRSQESGVRRRQESRSPRDSANIVVGTFRRTSKGFGFVRPQGA
                     RRGDKEGDIYIAASSTMDAADRDLVRVRLSRNKARGRGGQLRLAGEIVEIVERDTHRF
                     VGVYQERGGTSYVQVDGKVFAQLVPVGDPGAKGAAVGDKVVIEMVRFPTHQHAGEAVI
                     VEVLGAKGAPGVDTLSIIREFGLPEEFPDDVLEESREQADAFDESIGNRLDLTGQTIV
                     TIDPVDARDFDDAVSLKRLENGHWSLGVHIADVAHFVRSKSALDREAKERGTSVYLPD
                     RVIPMLPEIISNNLASLQPDKVRYTLSALMEFTADGAYVGGEVKRTAIRSCRRFAYEE
                     VDEYLADRAAWREKLTPQVWRLLADMHELAMILRRRRLEGGSIELTLPEVKIDLDKRG
                     EVVGAHLVQNTESHQVIEEFMLAANECVARMLGEAGLNFLRRVHEPPEPRKLAALTTF
                     VRELGIETESLESRFEIKRVVAAVAGQPEAHAVNYAVLRSMQKAVYAPTDEGHYALNK
                     EHYCHFTSPIRRYPDLTIHRMIETLARGKKPADDYDRMVVLGDQCSDREQRAEAAERE
                     LNKVKLLTYLSKRIGEQMDAVITGVEEFGLFAQGVELPAEGLIHVDSLGDDFYRFDRT
                     THSLEGHRSGNKFRLGDVIRVEVAHVDIDRRELDFRVVKKIGHAGKSQKAKTSRDQTS
                     SRRTAKRRR"
     gene            complement(3163..>3597)
                     /locus_tag="VFB80_13490"
     CDS             complement(3163..>3597)
                     /locus_tag="VFB80_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN34834.1"
                     /translation="VWGSSGGGSLTLSLLFRYPDLYRTGMAIASVPDMRLYDTIYQER
                     YMGLPQENAEDYKQGSPITHAEGLKGNLLLVHGSGDDNVHAQGMEKLVDRLIELNKPF
                     TMMSYPNRTHALSDGEGKNTTRHMYELLTRYLNENLPPGPRP"
BASE COUNT          555 a         1222 c         1094 g          726 t
ORIGIN      
        1 gaccagtatt cgcggtgcac ggtagagcgc gaccactggt cgcggctttg gtccaaatta
       61 tcagccccct ggttgggcag gtgcgaactc gcgtattgcg taaattctcc gtcgcaactc
      121 ttgatgcgcc agcgacttgc ggcacggtgc ccgcaacccc ttaagtagta cttattacac
      181 ttagagaatc gccccgccgt ctcctcaaag cggcgaccac tggtcgcgct cttcccatgc
      241 agcgcgacca ctggtcgcgg ctttgggcga ctgcgtcatc gaattgccaa tgaccattgt
      301 cgacgccgaa cgcaagttcg cgtcggaccg aattgtaaca atccgttcag tacatgtcaa
      361 gcgcgacaca acagattgtg tttggcctcc gcccaaccac aaccggtagt ggttgcgcca
      421 cctggcggcc cctgaaagaa gggtgagccc gtcccgggcc tagcggcggc gcttcgcggt
      481 ccgccgggaa gaggtttggt cccggctggt tttggctttc tgggattttc ccgcgtggcc
      541 gatctttttg accacgcgga agtccagctc gcggcggtcg atatcgacgt gggccacttc
      601 aacgcggatc acgtcgccga ggcggaactt attgccgcta cgatgccctt cgaggctgtg
      661 cgtcgtgcga tcgaagcggt agaagtcgtc gccgagcgaa tcgacgtgga tcagcccctc
      721 cgcgggcagc tcgacgccct gggcgaacag gccgaactcc tcgacgcccg tgatcaccgc
      781 gtccatctgc tcgccgatcc gcttcgacag gtacgtcagc agcttgacct tgttcagctc
      841 ccgctcggcg gcttcggccc gctgctcgcg gtcgctgcac tggtcgccca gcacgaccat
      901 ccggtcgtag tcgtcggccg gctttttgcc gcgggccagc gtctcgatca tccggtggat
      961 cgtcaggtcg ggatagcggc ggatcggcga cgtgaagtgg cagtagtgct ctttgttgag
     1021 ggcgtagtgc ccttcatcgg tcggagcgta aaccgccttc tgcatcgacc gcagcacggc
     1081 gtagttcacc gcgtgggcct ccggctgccc cgcgaccgcc gccacgaccc gcttgatctc
     1141 aaagcggctc tcgaggcttt cggtctcgat gcccagctcg cgaacgaatg tggtgagggc
     1201 agcgagcttc cgcggctccg gcggctcgtg gacgcggcgg aggaaattca gccccgcctc
     1261 gcccagcatc cgggcgacgc actcgttggc cgccagcatg aactcctcga tcacctggtg
     1321 gctctcggtg ttctgcacca ggtgcgcccc gacgacttcg ccgcgcttat cgaggtcgat
     1381 cttcacctcg ggcagtgtca gttcgattga gccgccttcc agccgccgcc ggcggaggat
     1441 catcgccagc tcgtgcatat cggccagcag ccgccagacc tgcggcgtca gcttctcgcg
     1501 ccacgccgcg cggtcagcca ggtattcatc gacttcctca taggcgaacc tgcggcagct
     1561 cctgatcgcc gtgcgcttca cctcgccgcc gacgtaggcc ccgtcggcgg tgaactccat
     1621 cagggccgag agcgtgtacc gcaccttgtc cggctgcagg ctggccaggt tgttgctgat
     1681 gatctccggc agcatcggga tcacgcggtc gggcagatag acgctcgtgc cgcgctcttt
     1741 ggcctcgcgg tcgagggccg acttggaccg cacgaaatgg gccacgtcgg cgatgtgcac
     1801 gccgagcgac cagtggccgt tttccagccg cttcagagaa actgcgtcgt cgaagtcgcg
     1861 ggcatcaacc gggtcgatcg tgacgatcgt ctggccggtc aagtcgagcc ggttgccgat
     1921 cgactcgtcg aacgcatcgg cctgttcgcg ggactcttcg aggacgtcgt ccgggaattc
     1981 ttcgggcagg ccgaattcgc ggatgatcga gagcgtatcc actcccggcg cgcccttcgc
     2041 gccgagcacc tcgacgatca cggcctcgcc ggcgtgctgg tgcgttggaa agcgcaccat
     2101 ctcgatcacg accttgtcgc cgaccgccgc gcctttcgcg ccaggatcgc cgacagggac
     2161 cagctgggcg aagaccttgc cgtccacctg cacgtagctc gtgccgccgc gctcttgata
     2221 gacgcccacg aagcggtgcg tgtcgcgctc cacgatctcg acgatctcgc cggcgagccg
     2281 cagttgcccg ccgcggccgc gagccttgtt ccggctcagc cgcacgcgga ccaggtcgcg
     2341 gtcggctgca tccatcgtgc tgctggcggc gatatagatg tcgccttcct tgtcgccccg
     2401 ccgcgccccc tgtggccgca caaacccaaa ccccttgctc gtccggcgaa acgtgccaac
     2461 gacgatgttc gcactgtctc tgggcgatcg gctctcttgc cgacgcctga cccctgactc
     2521 ctgactcctg tcccctgtcc cctgtcccct ggcttctggc tcctggctcc tggctcctgc
     2581 cgccggcttc ggtggccgca cctgatgccc cgcgccatag accagctttc ccgccttcac
     2641 cagccggcgg atcgccagct tcagcgcccg gtgctgctcg ctggcgagct tgagctgctt
     2701 ggcaatgacc cgcggcttca cgggctgata ttttggcccc aagacgtggg ccagcacgat
     2761 cggctccagc ttgcgggccg ccggcgaagc cttttcggcc tcagcctctt cttcgttttc
     2821 ctctttctcg acgacctctt ccgtcaattc ctcattcaca gcgttctttg gatcgaaact
     2881 catggactca atctatccgt tttccagtct gaagcgggca ggcaaaatcg cctgcgaaca
     2941 agtggccggc aaacgcgggc caagattggg gcgatttgcc ccatagagca tcccggtccg
     3001 cttcacgtcc taaagaggac agtcttgccg ggaaacacca gaaacctgag gccaggaagg
     3061 cccaagatgc ggacttcttc gttccgctcg attcatcttc atccagtata gcatcgattc
     3121 gccgcccggc cagggccttg ccgccgcggg acactcggga gcctacggcc gcgggccagg
     3181 cggcagattc tcgttgaggt atcgggtcag cagctcgtac atatgccgcg tcgtgttctt
     3241 gccctcgccg tcgctgaggg cgtgggtccg gttcgggtag ctcatcatcg tgaacggctt
     3301 gttgagctcg atcagccggt cgaccagctt ctccatgcct tgggcgtgca cgttgtcgtc
     3361 gccgctgccg tgcacgagca gcaagttgcc tttcaatccc tcggcgtgcg tgatgggcga
     3421 gccttgcttg tagtcttcgg cgttctcctg gggcagcccc atgtatcgct cctgatagat
     3481 cgtgtcgtac agccgcatgt ccgggacgga ggcgatcgcc atgccggtgc ggtacaagtc
     3541 ggggtaacgg aagagcaggc ttagcgtcag cgatccgccg ccgctgctgc cccagac
//
LOCUS       DASRJB010000515         6035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_143214, whole genome shotgun sequence.
ACCESSION   DASRJB010000515 DASRJB010000000
VERSION     DASRJB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143214"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..677)
                     /locus_tag="VFB80_13495"
     CDS             complement(84..677)
                     /locus_tag="VFB80_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34835.1"
                     /translation="MSDALINDSSGAAPPPAGSNIQIERFPDGVTINVPAQGVWKATK
                     GLFVFAIIWNGIVAMISMCMLAGIIGATQAVPVKKADATALWIVPLIFSLFWAVGIGL
                     LLAAINMGKRRAALAVTGGSLLVIQTGLFGSKRRTWESGEVESVCTGPSGLTVNDVPV
                     LELQIHDGGGKFGLLCGRADPDLECIAAELRQALQLK"
     gene            732..1631
                     /locus_tag="VFB80_13500"
     CDS             732..1631
                     /locus_tag="VFB80_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZN34836.1"
                     /translation="MPAPVPSTSQPPESISGPKPRAYLLGSGDKPNLREEAERLRPTI
                     QQHLEVVLADFQYQEDLSVVAADLAVVLGGDGSILRAALQMRERQIPILGVNLGKLGF
                     LASVSPDELPGVLPQVTGGEARVVEHLMFRCTILRDGQPVAETLGLNEVAIFVGPPFA
                     MLHAHLYVDAELATTYACDGLIISTPVGSTAHSLSAGGPILRKTLAAFVISPICPHTL
                     TVRPVVDTADRVYEVVVPQPGEGTTVVVDGRPLWKLAANDRVRVIRAEPTFKLLECRG
                     QSYYRTLRDKLGWAGNLYHKERP"
     gene            1720..2676
                     /locus_tag="VFB80_13505"
     CDS             1720..2676
                     /locus_tag="VFB80_13505"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HZN34837.1"
                     /translation="MKKGLSLATLLAALIVMTLFSACVMAARGAEVLSACGADDVAAE
                     LEKAKQRLSPAYTLAYKFTAGEKFRTKVVHLATVETKIKGAEQTTKSRTISTRAWRIK
                     EVNPQGHITFENVVERVEMWNSVSGKEEVRYDSRTDENPPPGFETVAQSIGRVLATIT
                     IDPHGRILTRENAQLQFNPGIGELTIPFPATPVKPGASWSIPDELKMTTDDGKVKKVQ
                     TQQQYTLEKVETGVATIKVQTQILTPINDPKLQSQLVQRLQRGTIKFDLDAGRLIHKQ
                     MDIDEEVFAFSGADSHMQYLARLTEEPLKEETAAAPDAGQRK"
     gene            3144..4229
                     /locus_tag="VFB80_13510"
     CDS             3144..4229
                     /locus_tag="VFB80_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34838.1"
                     /translation="MDASDARYSLPAALADRPWRRHAANLASPPPTMLMQPELDLLHW
                     LTSQYYQGQGLIIDAGCFLGGSTSALASGLATNPAAHGRKRLIHCYDLFSTSGKLWYD
                     FLPQFNLGRDQSFEDRFRRNVAAHAERIEVHAGELLEQTWGQEPVEILFLDICKFPPL
                     HDHATRLWFPRLIPGRSILIQQDYGWWDYHWGNIMMEVYRDRFAVLDDVPVASRVYLC
                     THAISESEAAERVYARLSEVDKLRHMDAAIASVGHSSYREHLWLNAAELAKTLGREDL
                     IKRALVEIFSNPSPGPAGASARERFPQYFAGAGTLAAAAPPPPPPAPQPPAATGRRLD
                     RPAASRGSLSSRFARLWRKVRRAVGSR"
     gene            4403..>6035
                     /locus_tag="VFB80_13515"
     CDS             4403..>6035
                     /locus_tag="VFB80_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN34839.1"
                     /translation="MFFSRWSIRRKLLICLAIVLSLVAALAYSGFAGAYSYRELARTI
                     GVRATDLKLAEELTHSLNELRVSISQTLQLTDMPGLSGLTGVEQGALGMEFDKKLANI
                     ERNLLTFRKHVNNVTLSDARFGDRVHEQRTIANLQAALDRLKRVKSDPTFVFTVAQQG
                     VLWPEIDSMYVTASALQDTMHDRMRDFARDVKSEYRAWIFTEWTATVLAAIFLPVAIS
                     LFYDWIFQPLNVLIAGSRRIAQQDDFDHRIELATSDEMAELAAAMNEMTARFQEIRSD
                     LDAQVKQRTREVVRSEQLASVGFLAAGVAHEINNPLASIAWCAESLDSRLHETLHGET
                     RPEQVLLDGADVEVLRKYLRRIQDEAFRCKGITEKLLDFSRLGDVQKQETDLGELIQG
                     VIEMVRHLGKYRQKKIELDCQEYVVAAVNAQEIKQVALNLITNALDSLEPGGTVWISL
                     KKTGGSAELLVRDNGCGMTAEVLRHLFEPFFSRRRDGTGTGLGLSITYRIVTDHGGSI
                     EPASDGPGKGAAMRVLLPLVAKDKVHERNPEKRLQA"
BASE COUNT         1115 a         1997 c         1922 g         1001 t
ORIGIN      
        1 caagggattc ccccttctcc tccggcggcc gagctgccgc cgcagccagg tttcgttcca
       61 gtcccacgcc gtgagaggcc gcttcacttg agctgcaggg cctggcgcaa ttcggcggcg
      121 atgcattcca gatcggggtc ggcgcggccg cagagcaggc cgaacttgcc gccgccgtcg
      181 tggatttgca gctccagcac gggtacgtcg ttgacggtca gcccggaggg gccggtgcag
      241 accgactcga cctcgcccga ctcccacgtc cgccgcttgc tgccaaacaa gccggtttgg
      301 atcaccagca gcgagccgcc ggtcaccgcc agcgccgccc gccgcttacc catgttgatc
      361 gccgccagca ggaggccgat ccccacggcc cagaacagcg agaaaatgag cggcacgatc
      421 cagagagctg tcgcgtccgc ctttttcact ggcactgcct gggtggcccc gataattcca
      481 gccagcatgc acatcgagat catcgcgacg atcccgttcc agataatcgc gaacacgaag
      541 agccctttgg tggctttcca aactccctgc gcgggaacgt tgatcgtcac gccatcggga
      601 aaccgctcga tctgaatgtt cgagccggcg ggcggcggcg ccgcgccgga cgaatcgttg
      661 attagcgcat cggacatggc ggcatcatac cgagcgttcg gcatccgcgc cgactcgtgt
      721 atgatgagac catgcccgct cctgtgccct cgacgtctca gccgcccgaa tccatctccg
      781 gaccaaagcc gcgggcctat ctgctcggct cgggcgacaa gccgaacctc cgcgaagagg
      841 ccgagcggct gcggccgacg atccagcaac atctcgaggt cgtgctagcg gacttccagt
      901 atcaagagga cctgtcggtc gtcgcggccg acctggccgt ggtcttgggg ggcgacggat
      961 cgatcctgcg ggcggctctc cagatgcggg agcggcaaat cccgattctg ggcgtgaacc
     1021 tgggaaaact ggggtttctg gcgagcgtct cgcccgacga gctgccgggc gtactgccgc
     1081 aggtgacggg cggcgaggcg cgggtcgtcg agcacctgat gttccgctgc acgatcctgc
     1141 gcgacgggca gccggtggcc gagacgctgg gtctgaacga ggtggcgatc ttcgtcggtc
     1201 cgccgttcgc gatgctgcac gcgcatcttt acgttgacgc agaactggcc acgacttatg
     1261 cttgcgacgg tttgatcatc agcacgccgg tcggttcgac ggcgcacagc ttgtcggctg
     1321 gcgggccgat cctgcggaag accttggccg cgttcgtcat ctctcccatc tgcccgcaca
     1381 ccctcaccgt gcggcccgtc gtcgatacgg cggatcgcgt ctatgaagtg gtggtgccgc
     1441 agccgggcga ggggacgacg gttgtggtcg atggccggcc gctgtggaag ctggccgcga
     1501 acgatcgcgt gcgggtgatt cgggccgagc cgaccttcaa gctgctggag tgccgcgggc
     1561 aaagttatta ccgcacgctc cgcgacaagc tcggctgggc cggcaacttg tatcacaagg
     1621 aacgaccgta gatcccgctt gcgagcggga gctcggacga cgagccgatc ccgcgaacgg
     1681 aaaggtcccg cccggagggc gggaagcaag gacgcaatca tgaagaaggg cctctctctc
     1741 gcgacgctgc tggcggctct gatcgtgatg acgctcttct cggcctgcgt gatggccgcg
     1801 cggggggcgg aggtgctgtc cgcctgtggg gcggatgacg tcgccgccga gctggaaaaa
     1861 gccaagcagc ggctgtcgcc cgcctatacg ctggcctaca agttcaccgc cggcgaaaag
     1921 ttccgcacga aagtcgtgca cctggccacg gtcgagacga aaatcaaggg cgccgagcag
     1981 acgacgaagt cgcggacgat ctcgacccgc gcctggcgga tcaaggaggt caacccgcag
     2041 gggcacatca cgttcgagaa cgtcgtcgag cgcgtcgaga tgtggaacag cgtcagcggc
     2101 aaggaagagg tccgctacga cagccgcacc gacgagaatc cgccgccggg gttcgagacg
     2161 gtcgcgcaat cgatcggccg cgtgctggcg acgatcacga tcgacccgca cggccggatc
     2221 ctgacccgcg agaacgcgca gctgcagttc aaccccggca tcggcgagtt gacgattccg
     2281 ttcccggcga ctcccgtcaa gccgggcgcg agctggtcga tccccgacga gctgaagatg
     2341 acgacggacg acggcaaggt gaaaaaggtg cagacgcagc agcaatacac gctggagaaa
     2401 gtcgaaaccg gcgtcgccac cattaaggtc cagacgcaaa tcctgacgcc catcaacgac
     2461 cccaagctcc agtcgcagct cgtgcagcgg ctgcagcgcg gcacgatcaa gttcgacctc
     2521 gacgccggcc gcctgattca caagcagatg gacatcgacg aagaggtctt tgccttcagc
     2581 ggtgccgaca gccacatgca gtacctggcc cgcctcaccg aagagccgct gaaagaggaa
     2641 acggcggcgg ccccggacgc gggccagcgc aaatagggcc gcgaaccttc ttgccccgcc
     2701 gctgctcttg atttctgacg gcatcgtaac aatgatgttt ttagacgtgt cgcgctgtac
     2761 gcggcgcgac gggactagca agctcggagt aggccgcatc tttcggcatc gcggccgaaa
     2821 cggggtcatt tctagttgag aaaggaggtg gtctagtgag ttatgtctgt accagccgag
     2881 gtggcgcctg ctaatgcagc cggcgggctg ccgtaggcag ccaccaaaac ccctcgctca
     2941 cagatcgcct gtgatcgagc taccttgggc ccggcctccc cgtgtgggag gccgggcttt
     3001 tttgtgcgca gccaaagggc gaaagaacca aagagccaaa attccaaaat gaattcggtt
     3061 ctttggttct ttggttcttg gaatttctct tctgccgacg gcgccaaacg cctaaactcc
     3121 ctccgcaacc gggagtcgaa tgcatggatg caagcgatgc gcgatacagc ctgcccgcgg
     3181 ctcttgccga ccggccgtgg cggcggcatg cggccaacct ggccagcccg cccccgacga
     3241 tgctgatgca gccggagctg gacctgctcc actggctgac ctcgcagtac taccaagggc
     3301 agggcctgat tatcgacgcc ggctgtttcc tcggcggctc gaccagcgcc ctggcctcgg
     3361 gactggccac gaatccggcg gcgcacggcc ggaagcggct catccactgc tacgacctgt
     3421 tttctacgtc gggcaagctc tggtacgact ttctcccgca attcaacttg ggccgcgacc
     3481 agtccttcga ggaccgcttc cgccgcaacg tggccgccca cgccgagcgg atcgaggtcc
     3541 acgccggcga gctgctcgaa cagacctggg gccaggagcc cgtcgaaatc ctgtttctcg
     3601 acatctgcaa gttcccgccc ctgcacgacc acgccacgcg gctctggttc ccgcggctga
     3661 ttcccggccg ctcgatcctg atccagcagg actacggctg gtgggactac cactggggca
     3721 acatcatgat ggaggtctat cgcgaccgtt tcgcggtgct cgacgacgtg ccggtcgcca
     3781 gccgcgtcta tctctgcacg cacgcgatca gcgagtccga ggcagccgag cgggtctatg
     3841 cgcggctttc cgaggtggac aagctgcggc acatggacgc ggcgatcgcc tcggtcggac
     3901 attcgagcta ccgcgagcac ctctggctca acgcggccga gctggccaaa acgctcggcc
     3961 gcgaggactt gatcaagcgg gccctcgtcg aaatattctc gaatccctcg cccggcccgg
     4021 ccggcgcttc ggcccgcgag cggttcccgc agtatttcgc gggcgccggt accctcgcgg
     4081 cggcggcccc gccaccaccg ccgccagcac cgcagccgcc cgctgcgacc ggccgccgcc
     4141 tggaccggcc ggccgcatcg cggggcagtc tttccagccg cttcgcccgg ctgtggcgca
     4201 aggtgcgccg ggccgtcggc agtcgctagc gttggcagtg gtcagtagtc agtggtcagt
     4261 tggaacgcat cagacctcca actgaccact gacaaccgac cactgacctc ctgctagcag
     4321 acatcgctat ttagcgattg cttgtccgcc gctacactcc gcggcgattc cgggcgggat
     4381 ccgcgcgaac aaggaggcgc tgttgttctt ttcgcggtgg agcattcggc gaaagctgct
     4441 gatctgcctg gcgattgtgc tgtccctggt ggcggccctc gcctacagcg gctttgcggg
     4501 ggcctattcg taccgggaac tggcccgcac gatcggcgtc cgcgccaccg acctcaagct
     4561 cgcggaagaa ctgacgcaca gcctgaacga gctccgcgtc agcatcagcc agacattgca
     4621 actgaccgac atgccgggcc tcagtgggct gacgggcgtc gagcagggtg cgctggggat
     4681 ggagttcgac aaaaagctgg cgaacatcga aaggaacctg ctgacctttc gcaagcacgt
     4741 gaacaacgtc actctttcgg atgcacgctt tggcgatcgc gtccacgagc aacgcaccat
     4801 tgcaaatctc caagcagcct tggatcggct caagcgagtg aaatccgacc cgacgtttgt
     4861 gttcaccgtc gcccagcaag gggtgctgtg gcctgagatc gacagcatgt atgtcacggc
     4921 gagcgccctg caggacacca tgcacgatcg gatgcgcgac ttcgctcgcg acgtgaagag
     4981 cgagtaccgg gcctggatct tcaccgaatg gaccgcgacc gtgctggccg cgatcttcct
     5041 gcccgttgcc atctcgctgt tctacgactg gatctttcag ccactgaacg tgctgattgc
     5101 cggctcgcgg cggattgctc agcaggacga cttcgaccat cgcatcgagc tggccaccag
     5161 cgacgagatg gccgagctgg ccgcggcgat gaacgaaatg accgcccgct tccaggaaat
     5221 ccgcagcgat ctcgacgccc aggtcaagca acggacccgc gaggtcgtgc gcagcgagca
     5281 gctcgccagc gtcggcttcc tggccgccgg cgtggcccac gagatcaaca acccgctggc
     5341 ctcgatcgcc tggtgcgccg aatcgctcga ctcgcggctg cacgaaacgc tgcacggcga
     5401 gaccaggccc gagcaggttc tgctcgacgg ggccgacgtc gaagtgctgc gcaaatacct
     5461 ccgccgcatc caggacgaag ccttccgctg caagggcatc accgagaagc tgctcgactt
     5521 ctcgcggctc ggcgacgtgc aaaagcagga gaccgacctg ggcgagctga tccagggcgt
     5581 gatcgagatg gtccgccacc tgggcaagta ccggcagaag aaaatcgagc tcgactgcca
     5641 ggaatacgtc gtcgccgcgg tcaacgccca ggaaatcaag caggtcgcgc tcaacctgat
     5701 caccaacgcc ctcgacagcc tggagcccgg cggcaccgtc tggatctcgc tcaagaagac
     5761 cggcggctcg gccgagctgc tggtccgcga taacggctgc ggcatgacgg ccgaagtgct
     5821 gcggcatctg ttcgagcctt tcttcagccg tcgccgcgac ggcacgggga cgggcctcgg
     5881 cctttcgatt acgtaccgga tcgttaccga tcacggcggc tcgatcgagc cggccagcga
     5941 cggtccgggc aagggcgcgg cgatgcgcgt cctgctcccg cttgtagcta aggacaaggt
     6001 ccatgaaaga aatcctgaaa aacgactcca agccg
//
LOCUS       DASRJB010000516         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2965740, whole genome shotgun sequence.
ACCESSION   DASRJB010000516 DASRJB010000000
VERSION     DASRJB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965740"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFB80_13520"
     CDS             <1..824
                     /locus_tag="VFB80_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34840.1"
                     /translation="RFAPDGQEIVLSGDEPEEGPPRVGGAVFGPVRWHAADSGKLIRE
                     QRLASRARLITPSPDWSRLAVATQEVRDEDGGPPAIRLLDAKTGAETGHFDLEPTGWE
                     TVALAWSADGRSLFTATSKKVIQTDAATLKTIAEIKLPHFRLEPATKTPMESGLYRGA
                     FVRHGTEILTAGDMAEFYGWRLMGGERLWAVKTDGQHFRVLLPSPDERLLAAVCIAAD
                     KSAKLRLFDLASRRNIATFDLGRDHADRLAFSPDGSKLLAGCYDGTALVFDVSDL"
     gene            complement(919..1197)
                     /locus_tag="VFB80_13525"
     CDS             complement(919..1197)
                     /locus_tag="VFB80_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZN34841.1"
                     /translation="MVLDFAWWCIFGLIAGAIAQFQMPGKDPGHPVDIAITLVLGIVG
                     AVVGGLLCSRVFGWDDMVGVAGGAVVLLVLYRGLAEAGIGRSHAANWQ"
     gene            complement(1332..2156)
                     /locus_tag="VFB80_13530"
     CDS             complement(1332..2156)
                     /locus_tag="VFB80_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34842.1"
                     /translation="MGRPGVGAPGVGVAPGVGRPGVGAPGVGVAPGLGRPGTPGQNLA
                     QNRPGRIENRDQRQGDRTERRDEIRNQYNENNPGSFWQENPVFSTWAITRPFAWANWG
                     SVGSWCGYSEQAMPYSYGENVYYADDQVYYGDMPVATTEEYAQQAEALAATDPATPPA
                     KGDWLPLGVFALTQDGQASGAPPTLYMQLAISKQGVIAGTLKNTTSGKVDTLDGMADV
                     KSQRVAWTIAGQQRPIVETGVANLTQDTAPALIHFADGQTQQWLMVRMPETMPQPK"
     gene            complement(2123..2299)
                     /locus_tag="VFB80_13535"
     CDS             complement(2123..2299)
                     /locus_tag="VFB80_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34843.1"
                     /translation="MFRPAALPSLVEREPHAVAGRQLIFCSREPAGCSGLWLARLRSL
                     QRPAWVAPASVRLA"
     gene            complement(2381..2584)
                     /locus_tag="VFB80_13540"
     CDS             complement(2381..2584)
                     /locus_tag="VFB80_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34844.1"
                     /translation="MEAHARPRAAADSRRPLPLALPLQQRGPPHLRPGRVLPPHGRAQ
                     AQSLHDRAAQRPAPRDPAPPHPA"
BASE COUNT          447 a          952 c          858 g          423 t
ORIGIN      
        1 tccgctttgc acccgatggc caggagatcg tgctcagcgg tgatgagccg gaagaaggac
       61 cgccgcgagt gggtggtgca gtctttggac cggtccgctg gcacgcggct gacagcggca
      121 agctcattcg cgagcagcgg ctggcctctc gcgcccgctt aatcactccg tcgcccgact
      181 ggtcgcgcct ggccgtggcg acgcaggagg ttagagacga agacggcggc ccaccggcga
      241 tccgcctgct ggatgccaaa acgggcgcag aaaccggcca cttcgacctc gaacctaccg
      301 gctgggagac ggtcgccctg gcctggtccg ccgacggcag atcgctcttc acggcgacga
      361 gcaagaaggt gatccagacg gatgccgcca cgctcaagac aatcgccgag atcaagctgc
      421 cgcacttccg cctggagccg gccacgaaaa cgccgatgga aagcggactt taccgggggg
      481 cgttcgtgcg ccacggcacg gagattctaa cggccggcga catggccgag ttttatggct
      541 ggcgtttgat gggcggtgaa agactgtggg cggtgaaaac cgacggtcaa catttccgcg
      601 tgttgctgcc atcgcccgac gagcgactgc tggcggcggt ctgcatcgcc gccgacaagt
      661 ccgcgaagct gcggctgttt gacctcgcct cccgccgcaa catcgccacc ttcgacctcg
      721 gccgcgacca cgccgaccgc ctggccttct cgcccgacgg cagcaagctg ctcgccggct
      781 gctacgacgg cacggcgctg gtgtttgacg tgtcagacct ctgaccggcc cgcggatcgc
      841 ccgcggaatt ggctttcagg gccccgagaa acggcttcca gaatggacca cactagctag
      901 tccattcatc aaaattcttc attgccagtt cgcagcatgc gagcgcccga ttccggcctc
      961 agccaatccg cggtacagca ccaagagcac gaccgctccg ccagccacac ccaccatgtc
     1021 gtcccagcca aagacgcggc tgcagagcag tcccccaacc acggcgccga cgatgcccag
     1081 cacgagcgta attgcaatgt cgacggggtg gcctggatct ttgccgggca tctgaaactg
     1141 ggcaatcgct cccgcgatca atccgaaaat gcaccaccat gcgaaatcga ggaccatcgc
     1201 tccgtcctcc gcgaaggcgc ttgggcgtcg ccatgcttgg agcaattgcc ccagcgcccc
     1261 gccgcacata acaaacggcg tcaggcgctg gtcgtaacac tcttacggtc aggcaagtgg
     1321 aactgaatca gttacttcgg ctgcggcatc gtctcgggca ttcgcaccat cagccattgt
     1381 tgcgtctgac cgtcggcaaa atggatcagg gccggcgcgg tgtcttgcgt caggttggcc
     1441 acgccggtct cgacgatcgg acgctgctgg ccggcgatgg tccaggcgac tcgctggctc
     1501 tttacgtcgg ccattccgtc cagagtatcg accttacccg aggtcgtgtt cttcagcgtg
     1561 ccggcaatga cgccctgctt gctgatggcc agttgcatgt agagcgtcgg cggggcgcct
     1621 gaagcctggc cgtcctgggt gagggcaaac acgcccagcg gcagccagtc gccttttgcc
     1681 ggcggcgtgg ccgggtcggt cgcggccagg gcctcggctt gctgagcata ttcctcggtc
     1741 gtcgccaccg gcatatcgcc gtaatagacc tgatcgtcgg cgtagtacac gttttcgccg
     1801 tagctatagg gcatcgcctg ttcgctatag ccgcaccaac tgccgaccga gccccaattg
     1861 gcccaggcga agggccgcgt gatggcccaa gtcgaaaaga cgggattctc ctgccagaaa
     1921 ctgcccgggt tgttttcgtt gtattgattg cgaatctcat cgcgccgctc agtgcggtcc
     1981 ccctgccgct gatcgcggtt ctcgatccgg ccgggccgat tctgagccag gttctgaccg
     2041 ggcgtgcccg gacgtcccag gccaggagca acgccgacgc ccggagcacc gacccccggc
     2101 cgaccgacac ccggtgctac gcctacgcca ggcgcaccga cgccggggcg acccacgccg
     2161 ggcgctgcag cgatcgcagc cgcgccagcc acaggccgct gcaccccgcc ggctccctgc
     2221 tgcagaaaat cagctgccgc cccgccaccg cgtgcggctc ccgctccacc agcgacggca
     2281 gcgccgccgg ccggaacatc gaggaagttg ttcacctggc cggccgacgg gcgggcgccg
     2341 ccggcgacgt tgccagcgcc cgcccccgga cgcgctgcgc ctacgccggg tgcggcggcg
     2401 ccgggtcgcg cggcgccggg cgctgcgccg cccggtcgtg cagcgactgc gcctgtgctc
     2461 ggccgtgggg cggcaggact cggccgggcc gcaggtgcgg cgggccgcgc tgctggagcg
     2521 gcagcgcgag gggcagcgga cggcgagaat ccgccgccgc ccgcggccgg gcgtgcgcct
     2581 ccaccaccgc ctccgccacc tcctcgcccg ccaccacctc ctcctcctcc gccgcgggcg
     2641 taaatttgga actgcacgac taggacaccc agaccgacga
//
LOCUS       DASRJB010000517         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_992987, whole genome shotgun sequence.
ACCESSION   DASRJB010000517 DASRJB010000000
VERSION     DASRJB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992987"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /gene="tsaE"
                     /locus_tag="VFB80_13545"
     CDS             complement(<1..384)
                     /gene="tsaE"
                     /locus_tag="VFB80_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZN34845.1"
                     /translation="MSEIVIDATSEQDTDHLGADLAARLPAGAVVSLTGTLGAGKTRL
                     VQAIAAACGVPREEVVSPTFVLCQQYSGTRTINHLDAYRLKSEDEFRELGAAELLNSD
                     ALTIVEWGDKIASALPADHVRIDIEV"
     gene            complement(381..1307)
                     /locus_tag="VFB80_13550"
     CDS             complement(381..1307)
                     /locus_tag="VFB80_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZN34846.1"
                     /translation="MERELVRWLTQRIPSHPRLLVGLGDDAAIVRLPDESHLVATTDM
                     LMDGVDFESGKQPEARRIGRKALAVNLSDLAAMAAQPVAALVALALPRQGGEVLAKNL
                     YEGMLPLAKEFDCPIAGGDTNSWEGPLVISVTAFGAVPEGRRWRRSGARPGDAILVTG
                     SFGGSILGKHLDFQPRVREALWLAEHAEVHAAIDVSDGLSLDLARLCEASGCGAALQT
                     SAIPLADAAAELAATRGDAPLARALADGEDFELILAVPADQAQELVQAGALGTRLTQI
                     GNFIEQPGLFAVGPGERLEPLAVRGYEHRLDP"
     gene            complement(1390..2343)
                     /locus_tag="VFB80_13555"
     CDS             complement(1390..2343)
                     /locus_tag="VFB80_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34847.1"
                     /translation="MIRYRPFENWDPPALAEIWRTQRPIRGRMQSVTPQVLEQHVLAK
                     PWFDRQGLIVACDGRRPVGFAHAGFGADCEQRGLDCSQGTTCLVLIAPHDERPQIARD
                     LLAASEDYLRRKGARQLFAGSQFPLNPFYLGLYGGSDTAGVLNSNQGFVDLLAASGYE
                     PKQRRVLAGRPLAGFRPPVDRQQMQVRRRFKVAGPTDVLPDNWWDAGVWALHELSRFD
                     LVLPGGGEPIISATFWDIDPLAASWGVRTVGLVKLDDTPEAREEGLSLYLLADVLKQY
                     QTTGYAHFEALAPADDPSLRNLFDQLGLVEYDEGVLWRKRA"
     gene            complement(2497..2811)
                     /locus_tag="VFB80_13560"
     CDS             complement(2497..2811)
                     /locus_tag="VFB80_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN34848.1"
                     /translation="MSRWVRIAAASEVPEGAGGEFVAGDQIVALFHVGGAFYALDGIC
                     PHQGGPLGKGQLAGCIVTCPWHGFQFDVTTGQHQTSRSLVHGRFPVKVEAGEVWVDVG
                     RG"
     gene            3256..>3660
                     /locus_tag="VFB80_13565"
     CDS             3256..>3660
                     /locus_tag="VFB80_13565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZN34849.1"
                     /translation="MSCCQKAVPGISIDESPAAPGWIERAIRASGVRRAFWSWLAGRA
                     RQRPYLQMLARCVRLEGDIVECGVFRGQSLLRMAAWLQQQGIRKTVHGLDSFTGFPRE
                     EVGASDLAPGRSLRAVRGRFRTAASAPRRILRV"
BASE COUNT          605 a         1247 c         1216 g          592 t
ORIGIN      
        1 gacctcgatg tcgatccgca cgtggtcggc gggcaaggcg gacgcgatct tgtcgcccca
       61 ttcgacgatg gttagcgcat cggagttcag cagctccgcg gcgccaagct cgcgaaactc
      121 gtcttcgctc ttcaggcgat aggcgtcgag gtggttgatc gtccgcgtgc cgctgtactg
      181 ctggcagagg acgaaggtgg ggctgacgac ctcctcgcgc ggcacgccgc aagccgcggc
      241 gatcgcctgc acgaggcgcg tctttcccgc gcccagcgtg cccgtgaggg aaacaaccgc
      301 tcccgccggc aagcgggcgg ccaggtccgc tcccaagtgg tcggtgtcct gctcgctcgt
      361 cgcgtcaatg acgatctcgc tcatgggtcc agccgatgtt cgtagccgcg aaccgccagc
      421 ggctccagcc gctcgcccgg cccaacggca aacagccctg gctgctcgat gaagttgccg
      481 atctgggtca gtcgcgtgcc gagggcaccg gcctggacga gctcttgggc ctgatcggcc
      541 ggcacggcca ggatcaactc gaaatcctcg ccgtcggcca gggcccgcgc cagcggtgcg
      601 tcgccgcgcg tggccgccaa ttcggcggcg gcgtcggcca gcggaatggc gctggtctgc
      661 agcgccgccc cgcagccgct ggcctcgcag aggcgagcca gatcgagcga gaggccgtcg
      721 ctgacatcga tggccgcgtg gacttcggca tgctcggcca gccagagcgc ttcgcggacc
      781 cgcggctgaa aatcgagatg tttgcccaga atgctgccgc caaagctgcc ggtgaccagg
      841 atcgcgtcgc ccggccgagc gccgctgcgc cgccagcggc ggccctccgg gacagcgccg
      901 aaggcggtga cgctgatgac cagcggacct tcccagctgt tggtgtcgcc gccggcgatg
      961 gggcaatcga actccttggc cagcggcagc attccctcgt agaggttctt cgccagcacc
     1021 tcgcccccct gacgcggcag ggccagcgcg accaaggccg ccacgggctg cgcggccatc
     1081 gccgccaggt cgctcaggtt caccgccagc gccttgcggc cgatgcggcg cgcctcaggt
     1141 tgttttcccg actcgaaatc gacgccgtcc atcagcatgt ccgtcgtcgc gacaaggtgg
     1201 ctctcgtccg gcaggcgcac gatcgccgcg tcgtcgccca agcccaccag caatcgcggg
     1261 tgcgacggaa tccgctgcgt cagccagcgc accagttcgc gttccatgga aaggtcgttg
     1321 ttcgaaaggt ttcggtggag caccgatttt ttggttcgaa gctgaaccct gaaccccgaa
     1381 ccctgaaccc taagctcgct tccgccacag cactccctcg tcatattcca ccaggcccag
     1441 ctggtcgaac aagttgcgga gactgggatc gtcagcgggg gccagggcct cgaagtgggc
     1501 gtagccggtg gtttgatatt gcttgagcac gtcggccagt aaatacaggg aaaggccttc
     1561 ttcgcgggcc tcgggcgtgt cgtcgagctt caccaggcca accgtccgca cgccccagct
     1621 ggctgccagc ggatcgatgt cccaaaacgt cgccgagatg atcggctcgc cgccgccggg
     1681 cagcaccagg tcgaaccgcg acagctcgtg cagggcccag acgccggcgt cccaccagtt
     1741 gtcgggcagc acgtcggtgg gcccggcgac cttgaatcgc cgccgcacct gcatctgctg
     1801 gcgatcgacc ggcgggcgga atcccgccag cggccgtccg gcgagcaccc gccgctgctt
     1861 gggctcgtaa ccgctggcgg cgagcaaatc gacgaaaccc tggttcgagt tgagcacccc
     1921 ggccgtgtcg ctgccgccgt atagccccag gtaaaacgga ttgaggggga actggctgcc
     1981 ggcaaagagc tgccgcgctc ccttgcggcg gaggtagtct tcgctggcgg ccagcaagtc
     2041 gcgggcgatt tgcggccgct cgtcgtgcgg cgcgatcaac accaggcacg tcgtcccctg
     2101 gctgcagtcc aggccccgct gctcgcaatc ggccccgaaa cccgcgtggg caaagccgac
     2161 cggccgcctc ccgtcgcacg cgacgatcag tccctgccgg tcgaaccagg gcttcgccag
     2221 cacgtgctgc tccagcactt gcggcgtcac ggactgcatc cggccccgga tcggccgctg
     2281 cgtccgccag atctcggcca gcgcaggcgg atcccaattc tcaaaggggc ggtagcggat
     2341 caagggaagc gcggggtcaa gtgatgggcg gaggccgccg cctgcaatca ggatacagga
     2401 gacaggggac aggagtcagg ggagtgacca ggggacagaa gccagtggcc aggagccaga
     2461 ttagatgcgt cccagcggtc catccgtccc atcctgtcac ccgcggccga catccaccca
     2521 tacttcgccc gcttccacct tcaccggaaa ccgcccatgc accagcgagc ggctggtttg
     2581 atgctggccc gtggtcacgt cgaactgaaa gccgtgccag gggcaggtga cgatgcagcc
     2641 ggcgagctgc cccttgccca gcggcccgcc ctggtgcggg cagatgccgt ccagggcata
     2701 gaacgcgccg cccacatgga agagggccac gatctggtcg ccggcgacga actctccgcc
     2761 ggcgccttcc ggcacttcgc tagcggccgc aatgcgaacc cagcgggaca tccccacagg
     2821 ataaccgacg gtgcccgaac cacggccttt gtgaacattg gtatgctatt tgcagtatag
     2881 ttccctttgg cgcggggcgg ctgccatttt ggctaccgcc gcccagccga gacgctcact
     2941 ggcaatttgg tcgttgacct ctggggcaga atcgccgccg ctgcccaggg cggcaaaata
     3001 taatggaaac tccccgcggg gttctctaag gcttatgctc ctaatgcgcg aaaatcgccg
     3061 agcgttacgc cgaaagtcat tgaccgccat gcagttgtga cgcgagcgac ggtgaaaagg
     3121 ggctcgctga gaattcccgc tcctgtcagt tttcgttatg ttcctagggc agctcgctcc
     3181 ctggcgactg tcgcaaagtg ccctttctcg gccgatttcc ccggagctat agtccgcccg
     3241 aaagggaggc ggagaatgtc gtgttgccag aaggcggtac caggaatctc gattgacgag
     3301 tcgccggctg cgccgggatg gatcgagcga gccattcggg ccagcggagt ccgccgggcg
     3361 ttttggagct ggctagcggg acgagcgcgg cagcggccgt acctgcaaat gctggcccgc
     3421 tgcgtgcggc tggaaggaga catcgtcgag tgcggcgtct ttcgcgggca gtcgctgctc
     3481 cgcatggcgg cctggcttca gcagcagggc atccgcaaga cggtccacgg cctggacagc
     3541 tttaccgggt ttccacgcga ggaggttggc gccagcgacc ttgctcccgg ccgctcgctg
     3601 cgggcggtcc gcgggcggtt ccgcacggcc gcaagcgcgc cgcggcgaat cctgcgcgtg
//
LOCUS       DASRJB010000518         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_707597, whole genome shotgun sequence.
ACCESSION   DASRJB010000518 DASRJB010000000
VERSION     DASRJB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707597"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /gene="priA"
                     /locus_tag="VFB80_13570"
     CDS             complement(<1..1897)
                     /gene="priA"
                     /locus_tag="VFB80_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZN34850.1"
                     /translation="MSSRQTTLFDAEPDPWQLDAAHEVLAASVVFAEQPLGPYDYAVP
                     ANLAAKLKPGQRVQVPLGKGNRSVTGYCTAVASKPVGSRPLKPIGRVIDERPLLSPAM
                     LRLAEWMADYYLCPLGQVLQAIVPAGVRGQAGTREMTFLSVPQQVREQLAAGALKLGQ
                     KQADALRVLAASARPLTPPELAQAAKCTLAPVNELRKKKLVQADVRRIQQVAAEQQPL
                     PRENHLQLNADQRTALDAILAPLKARRHETILLHGVTGSGKTEVYIRAIEEVLGYGRQ
                     AIVLVPEISLTPQTRSRFQSRFASVAVLHSHLSDAERHWHWQQIAAGLVQVVVGARSA
                     VFAPTPQLGLIVIDEEHDGSFKQNEAPRYHARDVALRRAAMESVPLVLGSATPSLESW
                     QKAAEGSGFRVQGSGIEIPNPKSQIANPQPPAPSPSPFVLIDLPRRVEDRPLPAVVTI
                     DLRAEFQSRSHRGAVSRSLQRAIEEALRDEGQVILLLNRRGFSTHIQCPACGHVVQCP
                     ACDLALTHHRQDEQAICHYCDFQTPAPVRCPECAFDGIRFSGFGTERLEAEIRGRFPD
                     VSVLRMDSDTMQRPGSHEAALSRFKSGEVKILLGTQMIAKGLDFPNVTLVGVINADTT
                     LHFADLRA"
     gene            complement(1907..3367)
                     /locus_tag="VFB80_13575"
     CDS             complement(1907..3367)
                     /locus_tag="VFB80_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN34851.1"
                     /translation="MSVRMITALVFLSCAAIAGHAGAADPPRPNVVFILADDLGWGDL
                     GCYGHPQTKTPHLDQLAREGTLFTQFYVCGSVCSPSRCAFFTGQYPARHKIHGHYATV
                     EQNSARGMTQFLDPAVPNTAALLKKAGYATAHVGKWHLGSNSGGPSPEKYGFDYVGST
                     ERGGADGPADDQYYRAKSTELFVNETLAFLKSHREQPCYVQLWTLVPHATLRPTEEQL
                     AAYPNLHPGGANFPHAAAAEIFSASVTDLDKQIGRLLAGIAELGIADKTLVLFSSDNG
                     PEDIHIKNAGHSGVGSAGPFRGRKRSLYEGGVRVPFIARWPGHVPAARIDDASVVAGC
                     DFLPTICQLAGAEIPANHALDGEDVGDILLGPSRPRTKPLMWNWRFRIAGEVFHHSPM
                     LAIRDGDYKLLLNPDRSRVELYDIPQDPTQLSNIAEKHPDIVARMSERVLAWHKTLPE
                     GPTDPGAGKASYPWPGKSAPAASPMGKGKGKSRAKQ"
     gene            complement(3430..4197)
                     /locus_tag="VFB80_13580"
     CDS             complement(3430..4197)
                     /locus_tag="VFB80_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34852.1"
                     /translation="MSAALKLPPSERDFEVFFKVMFHSVSTWRTAEFFNISQTRVRQL
                     VTLVGDWVADHLPDVGETDLQKQVRLAQHVAANRLEHQYEQVMALWTAEYNPKYLRQA
                     TRIALAQARLGLVTGRIHALAADVTAGPLELGDEIPPVDAHSPNRDCSPAASNGAGGR
                     AEDAEEIAASDGPEQMIERERYQRLRSVEGCSLMENRLLTLIANQGSHNPEKVAELEE
                     TLSRVRAEKATAELRLSRYVPGVQIAPLDVPREAALQ"
BASE COUNT          714 a         1505 c         1508 g          817 t
ORIGIN      
        1 ccgcccgcag atcagcaaag tgcagcgtcg tatcggcgtt gatcacgcca accagcgtca
       61 cgttgggaaa atcgagcccc ttggcgatca tctgcgtccc cagcaggatc ttcacttcgc
      121 cgctcttgaa cctggacagc gccgcctcgt ggctgccggg ccgttgcatc gtgtcgctgt
      181 ccatccgcag gacgctgaca tccggaaagc gcccgcgaat ctccgcctcc agccgctcgg
      241 tgccgaaccc ggagaagcgg atgccgtcga aggcacactc cgggcagcgg accggcgccg
      301 gcgtctggaa atcgcagtag tggcaaatcg cctgctcgtc ctggcggtga tgcgtcaggg
      361 ccaggtcgca ggccgggcac tgcaccacgt ggccgcaggc cgggcactgg atgtgcgtgc
      421 tgaagccgcg gcgattcagg agcagaatca cctggccctc gtcgcggaga gcctcctcga
      481 tcgcccgctg gagcgagcgg ctgaccgcgc cgcgatggct gcggctctgg aactcggccc
      541 gcaggtcgat cgtcaccacc gccggcagcg gccggtcttc cacgcggcgg ggcaggtcga
      601 tcagaacgaa ggggctaggg ctaggggctg ggggctgggg atttgcgatt tgggatttgg
      661 gatttgggat ttcaattcct gaaccctgaa ctctgaaccc tgagccctcc gccgccttct
      721 gccagctttc cagcgacggc gtcgcgctgc ccaggaccag cggcacgctt tccatggcgg
      781 cgcggcggag agcgacgtcg cgggcgtgat acctcggggc ttcgttctgc ttgaacgagc
      841 cgtcgtgctc ttcgtcgatc acgatcagtc ccagctgcgg cgtcggagcg aacaccgcgc
      901 tgcgggcccc cacgaccacc tgcaccaggc cggcggcgat ctgctgccag tgccagtgcc
      961 gctcggcatc ggacagatgc gagtgcagca ctgccacgct ggcaaaccgc gactggaacc
     1021 gcgagcgggt ctgcggcgtc aggctgatct ccggcaccag cacgatcgcc tgccgtccgt
     1081 accccagcac ctcctcgatc gcccggatgt agacctccgt cttgccgctg ccggtcactc
     1141 cgtgcagcag aatggtttcg tgccgccggg ccttgagcgg cgctaggatc gcgtccagtg
     1201 ccgtccgttg atcggcgttc agctgcaggt ggttttcgcg cggcagcggc tgctgctccg
     1261 cggccacctg ctgaatgcgg cggacgtcgg cctgcaccag cttcttcttc cgcagctcgt
     1321 tgaccggggc gagcgtacat ttagccgcct gggccagttc cggcggcgtc agtgggcggg
     1381 cggaagcggc gagcacccgc agggcgtcgg cctgcttctg gcccagcttg agcgcaccgg
     1441 ccgccagctg ctcgcggact tgctgcggca cgctgaggaa cgtcatctct cgcgtgcctg
     1501 cctggccgcg gacgcccgcc ggcacgatcg cctgcagcac ctgccccagc ggacagaggt
     1561 aatagtcggc catccactcg gccagccgca gcatcgctgg cgaaaggagc ggccgctcgt
     1621 cgatcacgcg gccgatgggt ttgagcggcc ggctgccgac cggcttgctg gccaccgccg
     1681 tgcaatagcc agtgaccgag cggttgcctt tgcccagcgg cacctgcacc cgctgcccgg
     1741 gcttcagctt cgccgcgagg ttcgccggca ccgcatagtc gtacggcccc agcggctgct
     1801 cggcgaacac cacgctcgcc gccagcacct cgtgcgcagc atccagctgc cacggatcgg
     1861 gctcggcgtc gaagagggtg gtttggcgag aggacattgt tcgattctac tgtttcgctc
     1921 tcgacttgcc ttttcccttg cccatcgggc ttgctgccgg cgctgatttg cccggccagg
     1981 ggtagctcgc tttgcccgcg cccggatcgg tcgggccttc gggcagcgtc ttgtgccagg
     2041 ccagcacgcg ctcggacatg cgggcgacga tgtcggggtg cttttcggcg atgttggaaa
     2101 gctgcgtcgg gtcttgggga atgtcgtaca gctccacgcg gctgcggtcc gggttcagca
     2161 gcagcttata gtcgccgtcg cggatggcga gcatcgggct gtggtgaaag acctcgccgg
     2221 cgatgcggaa ccgccagttc cacatcagcg gtttcgttcg cggccgtgat gggccgagca
     2281 ggatatcgcc cacgtcctcg ccgtcgagcg cgtgattcgc tggaatctcc gcgccggcca
     2341 gctggcagat cgtgggcagg aagtcgcagc cggcgacgac cgacgcgtcg tcgatccgcg
     2401 cggcgggcac gtgccctggc cagcgggcaa tgaatggcac gcgaacgccc ccttcataga
     2461 ggctccgctt gcggccgcga aaaggaccgg ccgagccgac gccgctgtgg ccggcgttct
     2521 tgatgtgtat gtcttccggg ccgttgtcgc tcgagaagag aaccagcgtc ttgtcggcga
     2581 tgcccagctc tgcgattccc gccagcagtc ggccgatctg cttgtcgagg tcggttaccg
     2641 aggccgagaa gatttcggcc gcggcggcgt gcgggaaatt cgccccgccg ggatgcaggt
     2701 tgggatacgc tgcgagctgc tcctcggttg ggcggagcgt cgcgtgcggc acgagcgtcc
     2761 acagctgcac gtagcacggc tgctcacggt gcgacttcaa aaacgccagc gtttcgttga
     2821 cgaacagctc cgtcgatttg gcccggtagt actggtcgtc ggccggtccg tcggccccgc
     2881 cgcgctcggt gctgccgaca tagtcgaagc cgtatttctc gggcgaaggg ccgccagaat
     2941 tgctccccag gtgccacttg ccgacgtgtg ccgtggcata gcccgccttc ttcagcaggg
     3001 ccgccgtgtt gggcacggcc ggatcgagga attgggtcat cccgcgggcc gaattctgct
     3061 cgactgtggc atagtggccg tgaatcttgt gccgcgcggg atattgcccc gtgaaaaacg
     3121 cgcagcggct gggcgagcag accgagccgc agacgtagaa ctgcgtgaac agcgttccct
     3181 cgcgggccag ctggtcgagg tgcggcgtct tcgtctgcgg atggccatag caacctagat
     3241 cgccccagcc caaatcgtcg gccaggatga acacgacgtt cggccgcggc ggatcggcgg
     3301 ccccggcgtg cccagcgatc gcggcgcagc tcagaaacac gagggctgta atcattcgca
     3361 ccgacataga aaactccttc acccggcggc ttcgcggtcc cattgccatc atttgactgg
     3421 cggccttcgt cattgcaacg ccgcctcccg cgggacgtcc agcggcgcga tttgcacgcc
     3481 cggcacgtaa cgcgagagcc gcagctcggc cgtggccttt tccgcgcgca cgcgactgag
     3541 ggtctcctcg agctcggcta ccttctccgg gttatgcgag ccctgattgg cgatcagcgt
     3601 gagcaggcgg ttctccatga gcgaacagcc ctcgaccgac cgcagccgct gataccgttc
     3661 gcgctcgatc atctgttccg ggccatcact tgcggcgatt tcctcggcgt cctccgcccg
     3721 gccgccggct ccgttgctcg cggcgggtga gcagtcccta ttcggggaat gagcatccac
     3781 cggcggaatc tcgtcgccca gctccagcgg cccggcggtc acatcagcgg ctagcgcgtg
     3841 aatccggccg gtgaccaggc cgagccgggc ctgggccaga gcaatccgcg tagcttgccg
     3901 caagtatttt gggttgtact cggcggtcca tagcgccatc acctgttcgt attggtgctc
     3961 cagccggttg gccgcgacgt gctgggccag gcggacctgt ttctgcaagt cggtctcgcc
     4021 gacgtcgggc agatggtcgg cgacccaatc gcccacgagc gtcacgagtt gccgcacgcg
     4081 ggtctgtgaa atgttgaaga actccgcggt ccgccaggtc gaaaccgaat gaaacatcac
     4141 cttgaaaaac acctcaaaat cccgctcact cggcggcagc ttcagcgcgg cagacatcgg
     4201 ttgatctcct gtaggacggt ggtcggtaag tcggtggtcg gtaagtcggt aagtcggtgg
     4261 tcggtggtcg gtgagtcagt ggtcagcggt ttgcacgaca aaccgactca ccgactcacc
     4321 gactgaccga ccgaccgccc ggaccgggcc caagatcata cggagcgcta tggactagtc
     4381 tggtccattt gagtcaagcg aaaaaaagat ttatcgaaat agttgggccg ctttcttcgc
     4441 ggcggtcggg tgccactggc gtctcgccag tgtcgaacga gggagccagc gctggaccgc
     4501 caaaaggcgg ccccatggcc attttcgaga aaggcaactc ccta
//
LOCUS       DASRJB010000519         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_707598, whole genome shotgun sequence.
ACCESSION   DASRJB010000519 DASRJB010000000
VERSION     DASRJB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707598"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /locus_tag="VFB80_13585"
     CDS             <1..2382
                     /locus_tag="VFB80_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34853.1"
                     /translation="PPGMTRPAAIAQAAAPAPRPSDSRPLQPVVVPNAQAIVDPLENM
                     PQATPVNAQRPLRVAQPLAVAPAPTVQVRAPQPVTPAAKPAAETTPGAEQKRGIPWQQ
                     PVKIILGGILGLFLGNIVVYFLTGDDFTGILPSRKPPLQNQPVASRPVQPQAPRTNTS
                     FNSPTPTQPAAPIPATPTPAENNLVPPPAPTSGGFNEPTLPANAAEPVGPPQTPASIL
                     ATPSAPKTPVATPAAAAPTSPPPAVPAAPAGKQQPPKPDQQQKMLAELQTIYKAEFDR
                     AARPDGRAGFVAFLRKTARELKTEPLAQFVLLRQAYDAAIGQQDFLSAADTIDDLEKV
                     FEMDGFRLRQHLLTEASQAAKKPEERTLLIPFALDLADFAARAQRMEELPKLANIADS
                     LARTTADKDLKAQVTARAIELRKAAADFAQVAAARKRLAEDPANPAASLIDGKFRCFL
                     SGEWAAGLKLLAQCDHAALAAAAKLDLAGPSEAVKPAQIGDAWFDLGTADPALTLALV
                     RAAHWYRQSAASAQGLEKVKLDKRLEEIAAKNLPAPSAADAAAPQLPSARSMLARSKT
                     FERVDLLAALNDQTIRTSGWTVNPGYAHVSSADFARLQAPTSPTGEYQVGLRLRRTSS
                     SSSQNGMFVIGLPHARSQFLLVLDFPIPGQGYASFLTLSNAKRPEDNPTFQLQAGQTH
                     RLTTSREYFVACTVAPNQIQVLLDGESLINYQGDMNRLTMPREWSVPDARSLFLGSHQ
                     GSFSIYSWVMAPLRDASGNELPLETAPSSRSRPTLGDPRFNFPPPFSPKSKQN"
     gene            complement(2383..>3811)
                     /locus_tag="VFB80_13590"
     CDS             complement(2383..>3811)
                     /locus_tag="VFB80_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HZN34854.1"
                     /translation="GYAFALILIPLGPLAVLAHIGELPLARDILMVGLALFVPVGLLV
                     LWILRLFSPRMVDYDSRSALLVNVARGFAEAVEGRKLPGLPGKLPSGQVLPGQAHTGI
                     GPNWVLIGAVFGGCAVLMLGCVGMMLVAGAIGRAGRAQQLAQTEARFKQMQAGAEARR
                     DQFLAERGGPRPPNWAAPPVGPPSPPPSVSNTAPPPAISAAPPDPLRGFGNPATTPVP
                     ATAPPAAFPTSPPGSSETTPPVATGAPTPAETKPAEPAPNPVPTRPRNPGFPPDFPPG
                     FPSFPEGTPGPPDVPGFGGGFGAGNPPAEMPRRNGSRRPSGGFGSDEQREEFRPGRGG
                     QFPPKSKQVAAASQLQPGAQVWVLWGPVWYRSTVVDSAGRQAAKVHYLGWGSNFDQVV
                     PLEGIRLATDAASSPGSEAPEGGNQPAHSRTWTDATGQFKVEAEFVEMQGNIVVLKKT
                     ADGQAIRIPLDKLSAEDQKTAKQLK"
BASE COUNT          622 a         1375 c         1243 g          571 t
ORIGIN      
        1 ccgcccggaa tgacccggcc tgctgcgatc gcgcaagccg cagcgcccgc gccgcggccg
       61 tccgattcgc gcccgctgca gccggtggtc gtgcccaacg cgcaggcgat tgttgatccc
      121 ttggaaaaca tgccgcaagc gacgccggtg aatgcccagc gcccgctccg cgtggctcag
      181 ccgctggccg ttgctccggc gcccaccgtt caagtccgcg cgccgcagcc ggtgacgcct
      241 gctgcgaagc cagctgccga gactacgcca ggcgccgagc agaagcgcgg catcccctgg
      301 cagcagccgg tgaaaatcat cctgggcggc attctcggac tgttcctcgg caacatcgtc
      361 gtctatttcc tgacgggcga cgacttcacg ggtatcctgc ccagccgcaa gccgccactg
      421 cagaatcagc ccgtggccag ccggcctgtg cagccacagg cgccgcgaac caatacgtca
      481 tttaactcgc cgacgccgac ccagcccgcc gcgccgatcc ctgcgacgcc aactccggcg
      541 gaaaacaacc tcgtcccgcc acccgcgccc accagcggcg gcttcaacga acccacgctt
      601 cctgcgaacg cagccgagcc agtcgggccg ccgcaaactc ctgcgagcat cctagcaacg
      661 ccgagcgcac cgaagacgcc ggtcgcaacg ccggctgcag ccgctcctac gtcgccgccg
      721 ccggctgtgc cggccgcccc cgcggggaag cagcagccgc cgaagcccga ccagcagcaa
      781 aagatgctgg ccgagttgca gacaatctat aaggccgagt ttgatcgcgc cgccaggccc
      841 gacggccggg ctggcttcgt cgcctttctg cgcaagaccg cccgcgagct gaagaccgag
      901 ccgctggccc agttcgtcct gttgcggcag gcctatgacg cggcgattgg ccagcaggac
      961 tttctctccg ccgccgacac gatcgacgac ctggaaaagg tattcgagat ggacggcttc
     1021 cgcctgcgtc agcacctgct gaccgaggcc agccaggcgg cgaaaaagcc cgaagagcgg
     1081 acgctgctga ttccgtttgc gctcgacctg gccgatttcg ccgcccgcgc gcagcgaatg
     1141 gaggagctgc ccaagctggc caacatcgcc gacagcctgg cccgaacgac ggccgacaag
     1201 gacttgaaag cccaggtgac ggcccgggcc atcgagctcc ggaaggcggc cgccgacttc
     1261 gcccaggtcg ccgcggcccg caagcggctg gcggaggacc cggccaatcc ggcggcgtcg
     1321 ctcatcgacg gcaaattccg ctgcttcctg tccggcgaat gggccgcggg gctgaagctg
     1381 ctggcccagt gcgatcacgc ggcgctggcc gcggccgcga agctggacct ggctgggccg
     1441 agcgaggcgg ttaaacccgc gcagatcggc gacgcctggt tcgacttggg cacggccgac
     1501 ccggcgctga cgctggcgct cgtgcgggcc gctcactggt atcggcaatc ggcggccagc
     1561 gcgcagggcc tggagaaagt caagctcgac aagcggctgg aggagatcgc ggccaagaac
     1621 ctgcccgctc cgagcgcggc cgacgccgcc gcgccgcaat tgccctcggc ccgctcgatg
     1681 ctggcccgct ccaagacgtt cgagcgggtc gacctgctgg cggcgctcaa cgatcagacg
     1741 atccgcacct cgggctggac ggtgaacccc ggctatgctc acgtcagctc cgccgatttc
     1801 gcccgcctgc aagcgccgac cagcccgacg ggcgaatacc aggtgggctt gcgcctgcgg
     1861 cggacatcgt cttcgtcgtc gcagaatggg atgttcgtga tcggcttgcc gcacgcccgc
     1921 tcgcagttcc tgctggtgct cgactttccc atcccgggac agggctatgc gagcttcctc
     1981 acgctcagca atgcgaagcg gcccgaggat aaccccacct ttcagctgca ggccggccag
     2041 acgcatcgcc tgacgaccag ccgcgagtat ttcgtcgcct gcacggtcgc gccgaaccag
     2101 attcaggtgc tgctcgacgg cgagtcgctg atcaactacc agggggacat gaaccgcctg
     2161 acgatgccgc gggaatggag tgtgcccgat gcgcggtcgc tcttcctcgg ctcgcaccag
     2221 gggagttttt cgatctacag ctgggtgatg gctccgctcc gcgacgccag cggcaacgag
     2281 ctgccgctgg agaccgcgcc ctcgtcccgc tcgcggccga cgctcgggga cccgcggttt
     2341 aactttccgc ccccgttctc tccgaagtcg aagcagaact agctacttca attgcttcgc
     2401 agtcttttga tcctccgcgc tcagcttatc gagcgggatg cggatcgcct gcccgtccgc
     2461 ggtctttttt agcaccacga tgttgccctg catttcgacg aactcggcct ccaccttgaa
     2521 ctggcccgtg gcgtcggtcc aggtccggct gtgggcgggc tggttaccgc cttcgggtgc
     2581 ttccgacccc ggcgagctcg ccgcgtcggt cgcgaggcga attccctcga gcggaaccac
     2641 ctggtcgaag ttgctgcccc agccgaggta atggaccttg gccgcctgcc ggcccgcgga
     2701 atccaccacc gtcgatcggt accaaaccgg tccccacaag acccagactt gtgctccggg
     2761 ctgaagctgg ctggcggccg ccacttgctt gctctttggc gggaactgcc cgccgcgacc
     2821 gggacgaaat tcttcgcgct gctcgtcgct gccgaagcct ccggagggcc ggcgcgagcc
     2881 attgcggcgt ggcatttccg ccggcgggtt gcctgcgcca aagccgccgc caaaaccagg
     2941 aacatccggc ggccccggag ttccctcggg gaaactggga aatcctggcg gaaagtccgg
     3001 cgggaacccc ggatttcgcg gccgggttgg gaccggattt ggcgctggtt ccgccggctt
     3061 cgtttcagca ggcgtcggtg cgccagtcgc gaccggcggt gttgtctcgc tggatcccgg
     3121 cggcgaagtt ggaaaggccg ccggcggggc ggtcgctggg actggagttg tagcagggtt
     3181 gccgaagcct cgtaaaggat cgggcggcgc cgcgctaatg gcggggggcg gcgccgtgtt
     3241 gctgacgctg ggaggtggcg atggcgggcc aaccggcggt gccgcccaat tcggcggcct
     3301 tggcccgccg cgctcggcca ggaactggtc gcgccgcgct tccgcaccag cctgcatctg
     3361 cttgaagcgc gcctccgtct gcgccaactg ctgggcgcgg ccggcgcgcc cgatcgcacc
     3421 cgcgaccagc atcatgccga cgcagcccag catgaggacc gcgcacccgc cgaacaccgc
     3481 gccgatcaag acccagttcg gcccgattcc ggtgtgtgcc tggccgggaa gcacctggcc
     3541 ggagggcagt ttgcccggca atcccggcag ctttcgcccc tcgacggcct cggcaaagcc
     3601 gcgggccaca ttcaccagca gcgccgaccg cgagtcgtaa tcgaccatcc gcggcgagaa
     3661 caaccgcagg atccacagca ccagcaagcc cacgggcacg aatagcgcca ggccgaccat
     3721 caggatgtcg cgggccagcg gcagctcgcc gatgtgggcc agcacggcca acggaccgag
     3781 cggaatcaag atgagtgcga acgcatagcc c
//
LOCUS       DASRJB010000520         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1554407, whole genome shotgun sequence.
ACCESSION   DASRJB010000520 DASRJB010000000
VERSION     DASRJB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554407"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFB80_13595"
     CDS             complement(<1..950)
                     /locus_tag="VFB80_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34855.1"
                     /translation="MAIDTPARLAILGAGPIGLEAALYARFLGYDVAVFEAGEVAASV
                     RRWGHVQMFTPFAMNHSPLGLAAIQAQDDSYQPPAEDALLTGREWVQRYLLPLAHTDL
                     LAEHLRLHTTALAVGKEELLKGDMPGNRSPHAPGEESVSRSETATIADRGDWLFRVLS
                     RDAAGAEQIDTFDGVLDCTGVFGRHNWLGHGGMPAAGELALAGEIEYRLPDILGADRA
                     RYAGRHTLLVGGGMSAATNVVALADLAKSDPATRVTWITRREGPAAAGGPIAVIDNDR
                     LPQRAELARAANVLTLKPEAQAKESPVTYWPRTVVERIVKA"
     gene            1150..1476
                     /locus_tag="VFB80_13600"
     CDS             1150..1476
                     /locus_tag="VFB80_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6793 family protein"
                     /protein_id="HZN34856.1"
                     /translation="MPLFEVETNAHIIITWAADDNAANAVVREAYPSDKVIRLTKRPR
                     DTWVISKGALGLNNKQVDVCTVARDCLSKAAGDKVHAIRLYIQHTGADLEQARKVIES
                     NMVMGW"
     gene            1768..3201
                     /locus_tag="VFB80_13605"
     CDS             1768..3201
                     /locus_tag="VFB80_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34857.1"
                     /translation="MSIYSWRWSLAVLAVVACSPFAQAQQKPSAVKVAEPVAAPQDAK
                     KPAAVAPAKSAPAPTPAGEPKAPPPVVAVEPEPAPPPPPQAAGAQPTPAQPGPAQPGA
                     AQPGPAQPAPLQHAPAPLPPAESSALVESDAPQESDAVIEVIKERYPNGAIKIEREVT
                     QDGEGNYLLHGAWRHFDDKGHLIIDGRLLRNRKEGVWRRFYRGDEVPLLASAPYKDFT
                     APFVSAATFHADRLHGRWTISDGRQRKVHEIEFTDGERHGKAIWYYPSGTVMLEAIYE
                     HGSVNGDVLKFAPDSSLLGRENFQTGRKLAPKVEFYDAAQQVKKLEAVYLHALLVIKT
                     PDSWERGALAVFENRGHDERHGAFALWHPNGQLARQGEFRYDHPVGPVNYWFANGQKQ
                     LEGQYVDGKQQGVWTWWHENGQKAITGEYHDGAAVGQWSWWNAAGKVAQKTDLTGERA
                     AVPQTPEAETELREAKLQLVEPGLPKR"
     gene            3198..3842
                     /locus_tag="VFB80_13610"
     CDS             3198..3842
                     /locus_tag="VFB80_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34858.1"
                     /translation="MSTAPQATWRFVVRAYLDAQYEDARQRHASELMNCEARCLREFG
                     AEPLWKEYEQLIQRRIAALGNTPPESAYEVIEESGERVVALVAAQRGNHGSGIPFLTT
                     RFVLSRGATAWKIDEILRPCFGCNPLLFEGRELRSGRRVVGSCFFCSGTGTPPWMQPR
                     GWWIFRRFTPSPEQCRFCHGTGRCKDCATSPERGWTKVYRSEKLADGESSRAEA"
BASE COUNT          718 a         1354 c         1410 g          655 t
ORIGIN      
        1 gccttaacga tccgctcgac gacggtccgc ggccagtatg tcaccggaga ctccttcgct
       61 tgcgcttcag gcttcagtgt gagcacgttc gcggcccgcg ccagctcggc ccgctgcggc
      121 aggcgatcgt tgtcaataac cgcaatcggc ccgccggccg cggccggtcc ttcgcggcgc
      181 gtgatccagg tgacgcgcgt cgccgggtcg gatttcgcca ggtccgccag cgcgaccacg
      241 ttcgtcgccg ccgacatccc cccgccgacc agcagcgtgt gccggccggc gtagcgggcg
      301 cggtccgctc ccaggatgtc cggcaggcga tattcgatct cgccggccag cgccagctca
      361 cccgccgccg gcatgccgcc gtggccaagc cagttatgcc gcccgaacac gcccgtgcag
      421 tcgagcacgc cgtcgaacgt gtcgatctgc tccgcgccgg ccgcatcgcg cgacaggacg
      481 cgaaataacc agtcgccgcg gtcggcaata gtagccgtct cgctccgcga gacgctttcc
      541 tcaccaggag cgtgaggact acgattgccc ggcatatccc ccttgagcag ttcctccttc
      601 cccacggcga gggccgtcgt gtgcagccgc aagtgttccg ccagcaggtc ggtatgggcc
      661 agtggcagca gatatcgctg gacccattcg cggcctgtaa gcagcgcatc ctcagcgggc
      721 ggctgatagc tgtcgtcctg agcctggatc gcagccaggc cgagcgggct gtggttcatc
      781 gcgaaggggg tgaacatctg gacgtggccc cagcggcgga cgctggccgc gacctcgccg
      841 gcctcgaaca ccgccacgtc gtagcccagg aaccgcgcgt agagcgcggc ctccagcccg
      901 atcggaccgg ctccgagaat ggccaggcgg gctggggtgt caattgccat cgccgctact
      961 tgtcaatgct gggcgtctgg ggcatcctaa ccgatggcag aaggcgagcg cgactggccg
     1021 cggagtttgg gcggtggagt ggtacaatac gcagtagacg aaccgattac gggccggctc
     1081 ttctttcctc cggatcgcca ggttgccggc gagcacgccg gcccctagct tttgcaaagg
     1141 agttttccca tgccgctgtt cgaggttgaa acgaacgcac acatcatcat cacctgggcc
     1201 gccgacgaca atgccgccaa tgcggtcgtt cgcgaggcct atcccagcga caaggtcatt
     1261 cgcctgacca agcggccgcg cgacacctgg gtgatcagca agggggcgct ggggctgaac
     1321 aacaagcagg ttgatgtgtg caccgtcgcc cgcgactgtc tgtccaaagc ggccggcgac
     1381 aaggtgcacg cgatccgcct ctatatccag cacacgggcg cggacctgga acaggcccgc
     1441 aaggtcatcg aatcgaacat ggtcatgggc tggtaggccg ctcggcgtcg cgcgtcagga
     1501 ggctcggctc gaccccagcg gaagcgacga gtcgatggca agtttgacgg cggggcaaac
     1561 tttgccgcct gtgccggttg taggcggtga tccgtaattg ccggttagcg ctggcgtgct
     1621 gcaaatttcg gcggcggccg tcgattacga agagtggacc tgtaattgcc gcaaattcgc
     1681 ccagcgagca agatgcgagc aagtttggcc cgcccgcagg atgcggacgc cgtctgtgac
     1741 ggaccacggt tggcaaagga gtgcctgatg tcgatctatt cttggcgctg gagcctggcg
     1801 gttctggcag tcgtcgcgtg ctcgcccttc gcgcaggccc aacaaaagcc gagcgccgtc
     1861 aaagtggccg aaccggtcgc cgccccgcag gacgcgaaga agcctgctgc tgtggcgccg
     1921 gccaagagcg ctcccgcgcc gacaccggcc ggcgagccga aagcgccgcc gcccgtggtc
     1981 gcggtcgagc ccgaaccggc gccgcccccg cccccgcagg ccgccggggc gcagccgact
     2041 ccggcgcagc ctggtcccgc gcagccgggc gccgcgcagc ctggtccggc acagcccgct
     2101 ccgctgcagc atgctccggc cccgctgccg cctgccgaga gttcggcgct ggtcgaaagc
     2161 gacgcgccgc aagagagcga cgcggtgatc gaagtcatca aggagcgcta tcccaacggc
     2221 gcgatcaaga tcgagcggga agtgacccag gatggcgaag gcaattacct gttgcacggg
     2281 gcctggcggc acttcgacga caagggccac ctgatcatcg acggccggct gcttcgcaac
     2341 cggaaggaag gcgtgtggcg gcggttctat cgcggcgacg aagttccgct gctggccagc
     2401 gccccctaca aggatttcac ggccccgttc gtctcggcgg cgacgtttca cgccgaccgc
     2461 ctgcacggcc gctggacgat cagcgacggc cggcagcgga aggtgcacga gatcgaattc
     2521 accgacggcg agcggcacgg caaagctatc tggtactatc ccagcggcac cgtgatgctg
     2581 gaggcgatct acgagcacgg cagcgtcaac ggcgacgtgc tgaagtttgc ccccgattcg
     2641 agcctgctgg gccgcgagaa ttttcagacg ggacgcaagc tggcgccgaa ggtggagttc
     2701 tatgacgcgg ctcagcaagt caagaagctg gaggcggtgt acctgcacgc actgctcgtc
     2761 atcaagacgc ccgatagctg ggagcgcggc gcactggcgg ttttcgaaaa ccgcggccac
     2821 gacgagcggc acggagcgtt cgccctctgg catcccaacg gccagctcgc ccggcaaggc
     2881 gagttccgct acgaccaccc cgtcggcccg gtgaactact ggttcgccaa cggccagaag
     2941 cagctcgagg gccagtacgt cgatggcaag cagcagggcg tctggacctg gtggcacgaa
     3001 aacggccaga aggcgatcac cggcgaatac cacgacggcg cggcggtcgg ccagtggtcg
     3061 tggtggaacg cggccggcaa ggtcgcccag aagaccgacc tcaccggcga gcgggcggcg
     3121 gttccgcaaa cgccggaagc cgagaccgag ctgcgcgaag ccaagctcca gctcgtcgag
     3181 cccgggctgc cgaagcggtg agcaccgcgc cgcaggcgac ctggcgattc gtcgttcgcg
     3241 cgtacctcga tgcccagtat gaggacgcgc ggcagcgtca cgcctccgag ctgatgaact
     3301 gcgaggcgcg gtgcctgcgg gagtttggcg ccgagccgct ttggaaggag tacgagcagc
     3361 tcattcagcg ccggatcgca gcgctgggca acacgcctcc cgaatcggcc tatgaggtca
     3421 tcgaggagag cggcgagcgg gtcgtcgccc tggtcgcggc gcagcggggc aaccacggct
     3481 ccgggattcc ttttctcacg acccggttcg tgctcagccg tggcgcgacg gcctggaaga
     3541 tcgatgagat cctgcgcccc tgctttggct gcaacccgct gctctttgaa gggcgcgaac
     3601 tgcggtcggg ccggcgggtg gtcggtagct gcttcttttg cagcggcacc gggactcctc
     3661 cctggatgca gccgcggggc tggtggatct tccggcggtt cactccgtcc ccggagcaat
     3721 gcagattctg ccacggcacg ggccgctgca aggattgtgc gacctcaccc gaacgcggtt
     3781 ggacgaaggt gtatcggtct gagaaactgg cggacggaga atccagcagg gctgaggcgt
     3841 aagttgttgt gaagtaaggc gttagcgata agcgccgagg ggcgggaata atcgccggct
     3901 actgggtctc tatgatttgc cgcgaaggcc aggcaggggg gtcctgctga gtctaagtta
     3961 ttttgtggca ctggtttaag gacgaattcg atgaagaagc tctttgcatt ggtcgccgcg
     4021 tgcggcctgg gactgtcggc gattggctgt ggcggtgata cgcctgcgcc ggtcacaccg
     4081 gctccggtca caccggctcc aacccccgaa ggtggcacgc ccgctccggc acccgaa
//
LOCUS       DASRJB010000521         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_708020, whole genome shotgun sequence.
ACCESSION   DASRJB010000521 DASRJB010000000
VERSION     DASRJB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708020"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFB80_13615"
     CDS             <1..242
                     /locus_tag="VFB80_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZN34859.1"
                     /translation="PGVGPVDRRAQDAQPAAAARVRGVVAMKRPNDLCSCGSGKKFKR
                     CCRSKQFSPPIEVRVQRRNVFGRPQLFVILKPNVR"
     gene            246..1337
                     /locus_tag="VFB80_13620"
     CDS             246..1337
                     /locus_tag="VFB80_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34860.1"
                     /translation="MRCKDFVRLAVLLLALSVGPLLAQLPPPGLPVAGQVDQAEILRR
                     GDLVVEAGEGPRSAAERLFVAGMAPPVDDSDQFYLTIFGKSGDARTEALRKAFETDPA
                     LACFVAPQPGTSRPWAHFNVYHADDRTQQFRFQAFKIDPQKLPVIVFQPPRDGSYGGL
                     AQIDEGGQKVTRSAVIDRIDAAQLGSSEQIASRLRSSFWLWCQKLEESGFVPGQKIAR
                     RLHQEPELRLAASDSHGQIPGPVPRQQPFSPQFPAGGPAAAESNPWPGIGGPLTPSVP
                     TVWSFIATLASGFLVYRSLRQQQGQKLLIPESVVQPLAILLQLLGNQPAPPPAPQPGP
                     VLTPLPEGWKWALINGVPTPVPPAPPSSA"
     gene            1480..1704
                     /locus_tag="VFB80_13625"
     CDS             1480..1704
                     /locus_tag="VFB80_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34861.1"
                     /translation="MRRLLVMSFVVLLALPAAPAEACPLGRFFRQRRPRRAAAVFICP
                     SCQAPPQVGRPADHTIGHQAGPGEPLPPAP"
     gene            1705..2061
                     /locus_tag="VFB80_13630"
     CDS             1705..2061
                     /locus_tag="VFB80_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN34862.1"
                     /translation="MPSSAPRACRCGGKIIDGSCTRCTHQAARRAAPPRASAAKRGYG
                     RKWREASQAYLRKHPLCVHCQQRGKLTPATVVDHITPHRGDWDLFWDSSNWQPLCKPD
                     HDHKTATEDGGFGRSN"
BASE COUNT          308 a          767 c          721 g          320 t
ORIGIN      
        1 cacctggcgt cgggccagtg gatcgtcgtg ctcaagacgc ccagcccgcc gctgccgccc
       61 gtgtacgtgg agtggtggcg atgaagcggc cgaacgacct ttgcagctgc ggcagcggca
      121 agaagttcaa acgctgctgt cgctcgaaac aattttcgcc gccaatcgaa gtccgcgtcc
      181 agcggcgcaa cgtcttcggc cggccgcagc tgttcgtgat cctgaagcca aacgtgaggt
      241 gacctatgcg gtgcaaagat ttcgtgcggc tggccgtgct gctgctggct ctcagcgtcg
      301 gcccgctgct ggcccagctg ccgccgcctg gattgccggt cgccggccag gtggaccagg
      361 ccgagatcct ccgccgcggc gacctggtcg tcgaggccgg cgagggcccg cgatcggcgg
      421 ccgagcggct gttcgtcgcg gggatggccc cgcccgtcga tgacagcgac cagttctatc
      481 tgacgatctt cggcaagagc ggcgacgcgc ggaccgaggc cctgcggaag gcattcgaga
      541 ccgatccggc gctggcctgc ttcgtggcgc cccagcctgg cacgagccgg ccctgggccc
      601 atttcaacgt gtaccacgcg gacgaccgga cgcagcagtt ccgcttccag gccttcaaga
      661 tcgacccgca gaagctgccc gtgatcgtgt tccaaccgcc ccgcgacggc agctacggcg
      721 gcctggccca gatcgacgag ggcggccaga aggtcacgcg gagcgccgtg atcgaccgga
      781 tcgacgccgc ccagctgggc agcagcgagc agatcgcctc acggctccgc tccagcttct
      841 ggctgtggtg ccagaagctc gaggagtcgg gcttcgtgcc cggacaaaag attgcccgcc
      901 ggctgcacca ggagcccgag ctgcggctgg cggccagcga ctcgcacggc cagatccccg
      961 gcccggtgcc gcggcagcag ccgttctcgc cccagttccc ggccggaggt ccggcggcgg
     1021 ccgaatcaaa cccttggccg ggcatcggcg ggcccctgac gccgtcggtg ccgacggtct
     1081 ggagtttcat cgcgaccctg gccagcgggt tcctcgtcta tcgctcgctg cggcagcagc
     1141 aggggcagaa gctgctgatt cccgagtcgg tcgtgcagcc gctggcgatc ctgctgcagc
     1201 tgctgggcaa ccagccggcg ccgccgccgg cgccgcagcc cggcccggtg ctgacgccgc
     1261 tccccgaggg ctggaagtgg gccctgatca acggcgtgcc gacgcccgtc ccgcccgccc
     1321 cgccctcgtc cgcttagcgc gcctgctgga cgagtggacc cacgtgcccc agcgggccca
     1381 gcagaagatc cagttggcgg tcatgctcta cacgtcgtcc gtgtgcgcgg cgatgatcat
     1441 cgccgccttg atccgcgccc gtttcactcg gaggtcctga tgcgtcgcct gctcgtgatg
     1501 tcgttcgtcg tgctgctggc gctgccggcc gcacccgccg aggcctgccc gctgggccgc
     1561 ttcttccgcc agcgccgacc gcggcgagct gcggcggtct tcatttgccc gagctgccag
     1621 gccccacccc aggtcggccg gccggccgat cacacgattg ggcaccaggc cgggccgggc
     1681 gagccgctgc cgcccgcccc gtagatgccc tcgtctgctc cgcgcgcctg ccgctgcggc
     1741 gggaagatca tcgacgggag ttgcacgcgg tgcactcacc aggcggcccg gcgtgcagct
     1801 ccacccaggg ccagcgccgc caagcgcggc tacggtcgca agtggcgcga agcgagccaa
     1861 gcgtatctgc ggaagcaccc gctctgcgtg cactgtcagc agcgcggcaa gctgacgccg
     1921 gcgacggtcg tcgatcatat cactccgcac cgcggcgact gggatctgtt ctgggactcg
     1981 agcaactggc agccgctgtg caagccagac cacgatcaca agacggcgac ggaagacggc
     2041 ggctttggcc gatctaactg acccccaccg gtattgtggc tgtgggaacc ctggagaccg
     2101 cctcgcgagt cgggaa
//
LOCUS       DASRJB010000522         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2400182, whole genome shotgun sequence.
ACCESSION   DASRJB010000522 DASRJB010000000
VERSION     DASRJB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400182"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..1599)
                     /locus_tag="VFB80_13635"
     CDS             complement(340..1599)
                     /locus_tag="VFB80_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HZN34863.1"
                     /translation="MPARPSAPADGHVQRVELAGHIIDSLLLPKVLDHITAGGGAFRI
                     EQISIGQARQDPSYALIEVRAPSAERLQTILADIADHGAVAVDAQDARLVAADIAGAF
                     PEGFYSTTNQRTEVRIGDKWLEVQNQEMDCGVLIDKAAGTAKCAAMTDVKLGELYVVG
                     HAGVRVHPQERQAQAHGFEFMNSPVSTEKPKGVAIRQVAHELIRTRREGGKALLVGGP
                     AIVHTGSVEHLMALIRKGYIHRLFAGNALATHDIEQALFGTSLGVRLDSGDIIEAGHE
                     HHLRAINRIRRLGGIRAAVEGGDLKSGIMYECVQHNVEFLLAGSIRDDGPLPEVITDT
                     LVAQAKMREQIRDVTFCLMVATTLHSIAVGNLLPAWVKVVCVDINPSTVIKLADRGSF
                     QTVGLVTDVEPFFRALVSDVERLEKNG"
     gene            1818..>2579
                     /locus_tag="VFB80_13640"
     CDS             1818..>2579
                     /locus_tag="VFB80_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34864.1"
                     /translation="MPEPGKSDRWNSLLETLGVPVSEPAPSPSPPPEASLVGEAPPAA
                     PTKPQPVSMLPPEKPKPAPKPKPAPSPAKSPSYWSRIAGALGLEAPPEPEPEPPAAAE
                     APSAPPPRAPEPRQARPPRAEPPRAFERPPRPAREPEPQRPEDLPARAFDATPSRPAA
                     EPAAPPTLDSMFGPKQPDLDVFGLGRDADEPPRQREMPPPRRRDEDTRSREDEERGPV
                     LEYDVAGGSDLSLPEDATAEGGSPRREREERGEEGR"
BASE COUNT          416 a          877 c          849 g          437 t
ORIGIN      
        1 gacatggagc ctccagagaa tatgcggtac tcaccactca cgccctagtg ctcaaccgct
       61 cacagcaccc ggcattttgc cgctggacgc cgaggctggc aagcaacttg tacacatttc
      121 gttcacgtta cgcatactta cgtgaacagg cgttgccata agcccgtggg ccgcctcgac
      181 ttccgctgcc gcgcatcgcc gtaagttttt ttccgggccg ctagcggaat ggactagtgt
      241 ggtccattcg gggacccctc ttcagagggc gcgctacgcc tggcgccacg ccgcgcggtc
      301 gatttgcgcg gcgtctggtc ggtggagcgg acagccacgc tacccgttct tctccagtcg
      361 ctcgacgtcg ctgaccagcg cgcggaagaa cggctccacg tcggtgacca ggccgacggt
      421 ctgaaagctg ccgcggtcgg cgagcttgat gacggtgctg gggttgatat cgacgcagac
      481 gaccttgacc caggccggca gcaggttgcc cacggcgatc gagtgcagcg tcgtggcgac
      541 catcaagcag aaagtcacgt cgcgaatctg ctcgcgcatc ttcgcctggg cgacgagcgt
      601 gtcggtgatc acttcgggca gcgggccgtc gtcgcggatg ctgccggcca gcaggaactc
      661 gacgttgtgc tggacgcact cgtacataat gccgctcttc aggtcaccgc cttccacggc
      721 cgcgcggatg ccgcccaggc ggcggatgcg gttaatcgcc cgcaggtgat gctcgtggcc
      781 ggcttcaatg atgtcgccgc tgtcgagccg cacgcccagg ctggtgccga acagggcctg
      841 ctcgatgtcg tgcgtggcca gggcgttgcc ggcaaagagc cggtggatgt agcccttgcg
      901 gatcaacgcc attaggtgct cgacgctccc ggtgtgcacg atcgccgggc cgccgaccag
      961 cagggctttg ccgccctcgc gccgcgtgcg gatcagctcg tgggcgacct ggcgaatggc
     1021 gacccccttg ggcttctcgg tggagaccgg cgagttcatg aactcgaagc cgtgggcctg
     1081 cgcctggcgc tcctgcgggt gcacgcgcac gccggcgtgg ccgaccacgt acagctcgcc
     1141 gagcttgacg tcggtcatcg cggcgcactt cgccgtgccc gccgccttgt cgatcagcac
     1201 gccgcagtcc atctcctggt tctgcacctc cagccacttg tcgccgatgc ggacctcggt
     1261 tcgctggttg gtcgtgctgt aaaagccttc gggaaatgcg ccggcaatgt cggccgcgac
     1321 cagccgggcg tcttgcgcat cgacggcgac cgcgccgtgg tcagcgatgt cggccaggat
     1381 cgtctgcagc cgctcggccg acggcgcgcg gacttcgatc agggcatagc tggggtcctg
     1441 ccgcgcctgg ccgatcgaaa tctgctcgat gcggaacgcg ccgccgcccg ccgtgatgtg
     1501 gtcgagcacc ttgggcaaga gcaggctgtc gatgatatgg ccggccagtt cgacgcgctg
     1561 aacgtggccg tcggccggtg cggatgggcg ggcaggcatg ggattccttt cgcaacaatt
     1621 cagctcactg gcacattgta cgaaaaacaa atcccaaaga accaaagaac cgaagaacca
     1681 aacggcaagc ttgggatctg atcttcggtt cttgggttct ttggctcttt ggggtttccc
     1741 acgcgtcttt gcgcaatttg cctttggcgg tatattgccg gtttgaagcc aacaaaatca
     1801 tcacgagcgt ccgttgtatg cctgaacctg gaaagtctga tcgctggaat tcgctgctcg
     1861 aaaccctggg cgtgccggtg agcgagccgg ctccctcccc ctctccgccg ccggaagctt
     1921 cgctcgtggg cgaggccccg cccgctgcgc cgaccaaacc gcagccggtc tcgatgctgc
     1981 cgccggaaaa gcccaaaccc gcgcccaagc ccaagcccgc tccctcgccg gcaaagtccc
     2041 cgagctactg gtcgcggatt gccggggcgc tgggattgga agcgccgccc gaaccggagc
     2101 ccgagccgcc ggcagcggct gaagcaccat ccgctccgcc gccgcgagcg ccggagccgc
     2161 gtcaggctcg tccgccacgc gccgagccgc cgcgggcctt cgagcgccct ccacgtccgg
     2221 cgcgagagcc agaaccacag cggccggagg acttgccggc gcgggccttc gacgcgactc
     2281 cgtcgcggcc agccgccgaa cccgccgcgc cgccgacgct cgattcgatg ttcggcccca
     2341 aacagccgga cctggatgtg tttggcctgg gccgtgatgc agacgagccg ccgcggcagc
     2401 gcgaaatgcc gccgccgcgt cgccgcgacg aggatacgcg cagtcgcgaa gacgaggagc
     2461 gcggtcccgt gctggaatac gacgtggctg gcggctcgga cctgtcgctg ccggaagacg
     2521 ccactgccga aggtggttcc cctcgccgcg aacgcgagga gcgcggcgaa gaaggccgc
//
LOCUS       DASRJB010000523         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1835441, whole genome shotgun sequence.
ACCESSION   DASRJB010000523 DASRJB010000000
VERSION     DASRJB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835441"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFB80_13645"
     CDS             complement(<1..357)
                     /locus_tag="VFB80_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN34865.1"
                     /translation="MPAPAKNAIGIDLGTTFSVISQLDDLGRPQTLINSEGDKITPSV
                     VFFEGENIVVGKEAVKALSTDAEAVAECAKRDLGNRFFHKPLGGRRYPPEALQAWVLN
                     KLRADAQKQIGQITKAV"
     gene            523..1977
                     /locus_tag="VFB80_13650"
     CDS             523..1977
                     /locus_tag="VFB80_13650"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HZN34866.1"
                     /translation="MKSHVFSLVILLAAAPLALAQQPPTRDEARAALAKAVAFFDGTV
                     SVEGAYLWRYSEDLSLREGEEAATATTAWLQPPGTPAVGEALLAAYERCGEKFLLDAA
                     VHTARSLVKGQLVSGGWDNSMEFDPDKRKDHAYRADWPPADGARNTTTLDDDKTQSAI
                     RFLMHVDRALAFKDQPIHEATLYALAGLIKAQYPNGAWAQRFTGPPKAEDFPVIKANY
                     PDSWPRTFPGVNYAGFYTLNDNAQADAIEVMFEAAEIYGDKQYSDCGRRGGDFLILAQ
                     MPDPQPAWAQQYNAQMQPAWARKFEPPSITGGESQGAIRILMEVYRQTGDKKYLEPIP
                     RALEYLAESKLEGGRLARFYELKTNRPLYFTRQYELVYTADDLPTHYAFIIGGSNIAR
                     LKADYEELLATDPAKLKPAARPARYEMSAELSSAAAQAIAALDSRGAWIVDGRLRAAD
                     PERKAKRIITTTTFNRNVETLSRFLAASKGSNAP"
BASE COUNT          388 a          728 c          735 g          363 t
ORIGIN      
        1 gacggccttg gtgatctggc cgatctgctt ctgggcgtcg gcccgcagct tgttcagcac
       61 ccaggcctgc agggcttccg gcggatagcg gcggccgccc agcggcttgt ggaagaagcg
      121 gttgcccagg tcgcgcttgg cgcactcggc gacggcctcg gcgtcggtcg agagggcctt
      181 caccgcctcc ttgccgacga cgatgttctc cccctcgaag aacaccacgc tgggcgtgat
      241 cttgtcgcct tcggaattga tcagcgtctg cggccggccc aggtcgtcga gctgcgagat
      301 gaccgagaac gtcgtgccca ggtcgatgcc aatggcgttt ttcgcgggag caggcatgcg
      361 gctcttgaaa accgtagccg aactcgccag agttcggaaa tcggcgggga tctgaattct
      421 ggcgaattca gctacgggcc gcagccagtc gcggcccgga tcaactaaga ttggtccact
      481 ctaccaacgg ctggaaaccc tcacaacgac tgaggtgagg ccatgaaatc ccacgttttt
      541 tccctggtga tcttgctggc ggccgcgccg ctggcgttgg cccagcagcc gcccacgcgg
      601 gacgaggccc gcgcggcact ggcgaaggcg gtggccttct tcgacggcac ggtctcggtc
      661 gaaggcgctt acctgtggcg gtacagcgaa gatctgtcgc tccgcgaagg cgaggaggcg
      721 gcgacggcga cgactgcctg gctgcagccg ccgggcacgc cggcggtggg tgaggcgctc
      781 ttggcggcct acgagcggtg cggcgagaag tttctgctcg acgcagccgt gcacacggcg
      841 cggtcgctcg tgaaggggca gctcgtcagt ggcgggtggg acaactcgat ggagttcgat
      901 ccggacaagc ggaaggacca cgcctatcgg gccgactggc cgcccgcgga cggggcccgc
      961 aacaccacga cgctcgacga cgacaagacg cagtcggcga ttcgcttcct gatgcacgtc
     1021 gatcgggcgc tggcgttcaa ggaccagccg atccacgagg cgacgctcta cgccctggcg
     1081 gggctgatta aggctcaata tcccaacggg gcctgggcgc agcggttcac tgggccgccg
     1141 aaggcggagg actttccggt catcaaggct aactatcccg attcctggcc gcggaccttt
     1201 cccggcgtga actacgccgg tttctacacg ctcaacgaca acgcccaggc cgacgcgatc
     1261 gaggtgatgt tcgaggcggc cgaaatctac ggcgacaagc agtattccga ttgcggccgc
     1321 cgcggcggcg atttcttgat cctcgcccag atgcccgatc cgcagccggc ctgggcccag
     1381 cagtacaacg cccagatgca gccggcgtgg gcccgcaagt tcgagcctcc gtcgatcacc
     1441 ggcggcgagt cgcaaggggc gatccgcatc ctgatggagg tctaccgcca gaccggcgac
     1501 aagaagtacc tggagccgat tccgcgggcc ctcgagtatc tcgctgaatc gaagctggaa
     1561 ggcggccggc tggcccgttt ttatgagctg aagaccaatc gcccactcta tttcacgcgg
     1621 cagtacgagc tggtctacac cgccgacgat ttgccgacgc actacgcctt tatcatcggc
     1681 ggctccaata tcgcccgcct gaaggccgac tacgaagagc tgctcgccac cgacccggcg
     1741 aagctgaagc ccgccgcccg gccggcccgc tacgaaatgt cggccgagct ctcctccgcg
     1801 gcggcccagg caatcgccgc gctcgattcc cgcggcgctt ggattgtgga tggcaggctc
     1861 cgcgccgccg atcccgagcg gaaggccaaa cgaatcatca ccaccaccac gttcaaccgc
     1921 aacgtcgaaa cgctcagccg gtttctggcg gcgagcaagg gaagcaacgc cccgtagagc
     1981 aagaggcagc cgagggaagg actacttcgc gtcgcacggc gctgctggcg tgcagcgtga
     2041 ggtggaactg gccgtcgtcc ttaggaccat gcaagttcaa aagaccgctt tcggtggctt
     2101 gaccagctat gtcgtgcacc agccgggaga cgccccgccg cggctggcgg tcgtgctctg
     2161 ccacggctat ggggcgacgg ggaccgacct ggtcggcctg gtgcgcccgc tgct
//
LOCUS       DASRJB010000524         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1835516, whole genome shotgun sequence.
ACCESSION   DASRJB010000524 DASRJB010000000
VERSION     DASRJB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835516"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /locus_tag="VFB80_13655"
     CDS             <1..2117
                     /locus_tag="VFB80_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN34867.1"
                     /translation="QRAIQMLRSGAMPPEDAVQPSDAVRRKIASLIEKTIYNIDCDLP
                     ADPGRVTIRRLNRAEYNNTIRDLIGVSFRPADDFPSDDVGGGFDNIGDVLTLPPLLME
                     KYLAAAERIAEAAIVADTSLYLKSQYRDRTQLSSKGQASYDQDFRRWTLWPAASAGSD
                     FEFSRDGEYQLSAQARLPRGATEPVVVELRIDGRPLEQFEVVPDRDANEKLELKRRVT
                     GGTHRLSLHLVPTPTPAFLEVSTANVNVGALEVTGPLDLRPEDFPPTHQRLIAARPGD
                     KLSVAEAARKNLEPLLTRAFRRPATEGEVQRFATLAEDAVKAGDSFEQGMQVALTAAL
                     VSPHFLFRTELDARPQDPQAVRELGDYELASRLSYFLWSSMPDDELFALAKAGKLREP
                     ATLEQQIRRMLADPKSAALVDNFITQWLNLRLLDGAAPDPRVFPRFDNGLKSAMRRET
                     ELFAAAIIREDRSILDFVSGRFTYVNERLARHYGIDGIRGDEFRRVEFSDKQRSVAAG
                     GLRSGVLTQASILTLTSNPGRTSPVKRGKWILENLLGSPPPDPPPDVPDLEATQRSQP
                     NLPLRKQLEIHRQNAVCASCHKTMDQLGFGLENFDAVGRWRDRDGQFPVDASGTLPGG
                     AKFNGPLELARVLDKRRGEFARCLAEKMLTFALGRELSPPDRCAVDKMVDEVERGEYR
                     FSALVTAVVNSDPFRKRRGEAP"
BASE COUNT          389 a          747 c          758 g          352 t
ORIGIN      
        1 ggcagcgggc gatccagatg ctccgctcgg gcgcgatgcc gcccgaagac gccgtgcagc
       61 cgagcgacgc ggttcgccgc aagatcgcca gcctgatcga gaagacgatc tacaacatcg
      121 actgcgacct tccggcggac cccggccgcg tgacgatccg ccggctgaac cgggccgagt
      181 acaacaacac gatccgcgat ctgatcggcg tttcgttccg cccggccgac gactttccct
      241 cagacgacgt cggcggcgga ttcgacaaca tcggcgacgt gctgacgctg ccgccgctct
      301 tgatggaaaa atacctcgcg gccgccgagc ggatcgcaga agcggcgatc gtggccgaca
      361 cgtcgctgta cctgaagtcg cagtatcgcg accggacgca gctgtcgtcg aaggggcagg
      421 ccagctacga ccaggatttc cgccgctgga cgctctggcc cgccgccagc gctggcagcg
      481 acttcgagtt ctctcgcgac ggcgaatatc agctctcggc ccaggcccgg ctgccgcggg
      541 gcgcaaccga accggtggtg gtggagttgc ggatcgacgg ccggcccctg gagcagtttg
      601 aggtcgtgcc cgatcgggac gccaacgaga aactggaact caagcggcgc gtgacgggtg
      661 ggactcaccg cttgagcctg cacctggttc ccacgccgac gccggccttt ttggaagttt
      721 ccaccgcgaa cgtcaacgtc ggcgcgctcg aggtcactgg cccgctcgac ttgcggccgg
      781 aagactttcc gcccacgcat cagcggctga tcgcggcccg gcccggcgac aagctcagcg
      841 tggcggaggc ggcccgcaag aatctcgagc cgcttttgac gcgggccttc cgccgcccgg
      901 cgacggaagg cgaggtgcag cggttcgcga cgctcgcgga agacgcggtc aaggccggcg
      961 attcgttcga gcaaggcatg caagtcgcgc tgaccgccgc gctcgtctca ccgcacttcc
     1021 tgtttcggac cgagctcgat gcgcggccgc aagacccgca ggcggttcgc gaactgggcg
     1081 actacgaact ggcgtcgcgg ctctcgtatt tcctgtggag cagcatgccg gacgacgagc
     1141 tgtttgccct ggcgaaggcc ggcaagctgc gcgagccggc cacgctggaa cagcaaatcc
     1201 gccggatgct cgccgacccg aagagcgccg cgctggtgga caatttcatc acgcagtggc
     1261 tcaacctgcg gctattggac ggcgccgcgc ccgatccgcg ggtctttcca cggttcgaca
     1321 acggtctgaa gagcgcgatg cgccgcgaga ccgagctgtt cgccgcagcc atcatccgcg
     1381 aggaccgcag catcctggat tttgtcagcg gccgcttcac ctacgtcaac gagcgcttgg
     1441 ctcggcacta tggaatcgac ggcattcgcg gcgatgaatt ccgccgcgtc gagttcagcg
     1501 acaagcaacg cagtgttgct gccggcgggc tgcgcagtgg agtactgacc caggcgagca
     1561 tcctcacgct gaccagcaat cccggccgca cgtcgcccgt gaagcgcggc aagtggatcc
     1621 tggagaacct ccttggctcg ccgccgcccg acccgccgcc ggatgtgccc gacctggaag
     1681 cgacgcagcg gagccagccg aacctgccgc tccgcaagca gctggaaatc caccggcaga
     1741 atgcggtctg tgcatcctgc cacaagacga tggaccagct ggggtttggc ctggagaatt
     1801 tcgacgccgt cggccgctgg cgggaccgcg acgggcaatt cccggtcgat gcctcgggca
     1861 cgctgcccgg cggtgcgaag ttcaatggcc cgctggagct ggctcgcgtg cttgacaagc
     1921 ggcgggggga attcgctcgc tgtctggcgg aaaaaatgtt aacttttgcc ttgggccgcg
     1981 aattgtcgcc ccctgaccgc tgcgcggtcg ataaaatggt ggacgaggtc gagcgcgggg
     2041 aataccggtt ttccgcgcta gtaacggccg tcgtgaatag cgaccccttc cggaagcgcc
     2101 gaggcgaagc accatgagca ggaccatcag ccggcggacg atgctgcgag gcctgggttg
     2161 cgcgatgggg ctgccgctgc tcgaggcaat gaggccggcc cgcggttttg ccgccgacgc
     2221 ggcaaatagg ccgccggtgc gactgg
//
LOCUS       DASRJB010000525         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3809736, whole genome shotgun sequence.
ACCESSION   DASRJB010000525 DASRJB010000000
VERSION     DASRJB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809736"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFB80_13660"
     CDS             <1..217
                     /locus_tag="VFB80_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34868.1"
                     /translation="IQGGRVVGQTSPTPKLAENQWTEDVSDPRNIEDLNATILHALGI
                     DFKQEIETPIGRPMAFSKGKVIAELLA"
     gene            complement(214..1677)
                     /locus_tag="VFB80_13665"
     CDS             complement(214..1677)
                     /locus_tag="VFB80_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34869.1"
                     /translation="MQSQTLSRRQLLAQAGGGAGLLALAALLAEERWLGPAAAAETSG
                     ASADPRLNPLAPKKSHFPAKAKSVIWLFMNGGPSQVDTWDYKPELEKRDGKELEGFDK
                     NTGFFTAQVGPVMRSPFQFAQHGKCGQWVSDIFPRMAEHVDEMAFIRSCYTGSNNHSP
                     ALFMMNTGTTRMGYPSVGSWVTYGLGSESLNLPAFVAMTDPLGRGLPKGYSQNWSAGL
                     LPSVYQGTWLKPQGEAIDNLYRPKDQGEAQQRAALDLLSKLNKHRLEQTPQELELAAR
                     IESFELAYRMQMAAPEAIDVDREPESIHKLYGVGEKRCDHFARQCLVARRLVERGVRF
                     VQIYSGGMENERSWDGHQNIKDNHTQFAGETDTPIGGLLSDLKQRGLLDSTLVIWGGE
                     FGRLPIVQKGGTGRDHNPHAFTVWMAGGGVKGGTIYGATDELGHKAVENRVHVNDLHA
                     TILHLLGLDHLKLTFRMSGRDFRLTDVAGHVVKDILA"
     gene            complement(1732..>2652)
                     /locus_tag="VFB80_13670"
     CDS             complement(1732..>2652)
                     /locus_tag="VFB80_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN34870.1"
                     /translation="AADAPAKVAEEPPPAAAPAPPEAAVASVPPAGAGQTPPAPAWPP
                     GPIPGQFPPGYMPPPGFRPPPGYNPPPGFAPPNPFAASPPPGNAAKAAAAPRSSKAPS
                     APPRPAQTLDEIVELLKSGQPTFGRLYSPLHRLRGIEPVASRREEVAALLDPLLTTGD
                     FSVRHAAYDAVEVWGTQRNVPTLLAILDGSDRSERWPAMEALGKIGGSPEAAARLAKL
                     MLDSGESLQAKRALEDMGAVAEDPVWQLVGNSDNLIHSYACQVLGKVGTSKSLERLKS
                     RPKEAQTVRQVSVDLAVQDMQGRLRGQPQN"
BASE COUNT          416 a          869 c          882 g          485 t
ORIGIN      
        1 catccagggc gggcgcgtcg tcggtcaaac gtcgcccacg cccaagctgg ccgagaacca
       61 gtggaccgag gacgtatctg atccccgcaa catcgaggac ctgaacgcca cgatcctcca
      121 tgctctgggc atcgacttca agcaggagat cgaaacgcca atcggccgcc cgatggcgtt
      181 ctccaagggc aaggtcatcg ccgagctgct ggcctaagcc agaatgtcct tcacgacgtg
      241 tcccgcgacg tcggtcaggc ggaagtcgcg accagacatg cggaaagtaa gcttcaggtg
      301 gtcgaggccg agcaggtgca ggatcgtggc gtgcaggtcg ttgacgtgaa cgcggttctc
      361 gaccgccttg tggcccagct cgtcggtcgc gccgtagatc gtgccgccct ttacgccgcc
      421 accggccatc cagacggtga acgcgtgcgg gttgtggtcg cgaccggtgc cgcccttctg
      481 gacaatcggc aggcggccga actcgccgcc ccagatgacc agcgtgctgt ccaggaggcc
      541 gcgctgcttg aggtccgata gaaggccgcc gatcggcgtg tcggtctcgc cggcgaactg
      601 cgtgtggttg tccttgatgt tctggtgtcc gtcccagctc cgctcgttct ccatgccgcc
      661 cgaatagatc tgcacaaacc gcacgccgcg ctcgaccagc cggcgggcca cgaggcactg
      721 ccgggcaaaa tggtcgcagc gtttttcgcc aacgccgtag agcttgtgaa tcgactcggg
      781 ctcgcggtcc acgtcgatcg cttccggggc cgccatctgc atccgatagg ccagctcaaa
      841 actctcgatg cgggccgcca gctccaactc ctgcggcgtt tgttccagcc ggtgcttgtt
      901 gagtttgctc agcaggtcga gggccgcccg ctgctgagct tcgccttgat ccttgggccg
      961 gtagaggttg tcgatcgctt cgccttgcgg cttcagccaa gtcccctgat agacgctggg
     1021 cagaaggccc gcgctccagt tttgcgaata gcccttgggc aggccgcggc cgagcggatc
     1081 ggtcatggcg acgaacgccg gcagattgag actctcgctc cccaggccgt aggtcaccca
     1141 ggagccgacc gacgggtagc ccatccgcgt cgtgccggtg ttcatcatga acagggcggg
     1201 cgaatggttg ttgctgccgg tatagcagga gcggatgaac gccatctcat cgacgtgctc
     1261 ggccatccgc ggaaagatat cgctgaccca ctgcccgcac ttgccgtgct gagcaaactg
     1321 gaacggcgag cgcatcaccg ggcccacctg ggccgtgaag aagcccgtgt tcttgtcgaa
     1381 gccttcgagc tccttgccgt cccgtttttc cagctccggc ttgtagtccc aggtgtcgac
     1441 ctggctgggt ccgccgttca tgaacagcca gatcacgctc ttggccttcg ccgggaagtg
     1501 gctcttcttc ggcgcgagcg ggttcagccg cggatcggcc gaagcgccgc tcgtctctgc
     1561 cgccgcggct ggcccgagcc atcgctcctc cgcgagcagc gccgccagcg cgagcaagcc
     1621 cgccccgccg ccggcttggg ccagaagttg acggcgggac aaggtttgag attgcatggc
     1681 tgagttgtcc ggcgtgttat cagttagtgt atcgcagttc ctgcggcccg gctaattctg
     1741 cggttgtccc cgcagccgtc cctgcatgtc ttgaacggcg agatccaccg aaacttgccg
     1801 gacggtctga gcttccttgg gacgcgattt cagtctttcc aggctcttgg aggtccccac
     1861 ttttcccaac acttggcagg cataggagtg aatcagattg tctgaattcc cgacgagttg
     1921 ccagaccgga tcctcggcca ctgctcccat gtcttcgagc gcccgcttgg cctgcagcga
     1981 ttcgccgctg tcgagcatga gtttcgcgag ccgggccgcg gcctcgggcg agccgccgat
     2041 cttgcccagg gcctccatcg ccggccagcg ctcgctgcgg tcgctgccgt cgaggatggc
     2101 cagcagagtg ggaacgttgc gctgcgtccc ccagacttcg actgcatcgt aggcagcatg
     2161 gcggaccgaa aaatctcccg tggtgagcag cggatcgagc agcgccgcca cctcctcgcg
     2221 gcgggacgca accggctcga tcccgcgcaa acggtgcagg ggcgagtaaa gtcggccgaa
     2281 cgtcggctgg cccgacttca gcaattcgac gatctcgtcc aacgtctgcg ccggccgagg
     2341 aggagcactc ggcgccttgc tggatcgcgg agcagcggcc gccttggcgg cgttgcctgg
     2401 cggagggctc gccgcgaaag ggtttggcgg cgcgaatccg ggcggtgggt tatagccggg
     2461 gggcggccga aatccgggcg gcggcatgta gccgggcgga aactgccccg ggattggtcc
     2521 gggcggccaa gctggcgccg gcggcgtttg ccctgcgccg gccggaggaa cggaggctac
     2581 tgcggcttcg ggcggtgcgg gcgcggcggc aggaggcggt tcctcggcaa cctttgccgg
     2641 tgcatccgct gc
//
LOCUS       DASRJB010000526         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3246810, whole genome shotgun sequence.
ACCESSION   DASRJB010000526 DASRJB010000000
VERSION     DASRJB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246810"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFB80_13675"
     CDS             complement(<1..191)
                     /locus_tag="VFB80_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34871.1"
                     /translation="MKMEMMVRQTVRQAAADLVAALDACAARLAAGESEAASYAAVAA
                     ALDGCLAELRSLGLWGQDNR"
     gene            252..1658
                     /locus_tag="VFB80_13680"
     CDS             252..1658
                     /locus_tag="VFB80_13680"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34872.1"
                     /translation="MPVTVACRCGQRFAAPNHLQGKQVPCPVCKAPISIPAAGIKIAC
                     RCGMVSFAPDTLRGQTTRCPGCGDLIRVPALGTDDLQVAGPKPAPDPLGRGPRIAPPA
                     KSPWLIVGLVAGGCGLVVVMMIAALAVMALKNSLGQARRPTVPPPGFSTPPVDPGPGY
                     SRSNPPRPWSPPPTPPPSAYQPPPPPPTSIRPVPSNPSFPPFGLPPAPPSPPSSSPSS
                     SASSPPPAAPKKNVTLPEGIATWYEQPSLKLTGIRRVGPASPPSAHFSWMTGLLPFLD
                     HDETFDKLNFSQPLTQQANLQVGATVIPQFLNPLDSRQRWKGYPLDGLALTHFVGMSG
                     VEDARNVVAAKLPRSDPRAGVFGYDEVARAEQITDGQSQTIMVVGGGELANPWLFGGG
                     ATIRGAREPLFDKTSGLGTKGLAGGGTLAIMADGSVRQINPQIDPAVFKAMCTIHGSE
                     KVDLEKSATPFDWELLKK"
     gene            1859..3274
                     /locus_tag="VFB80_13685"
     CDS             1859..3274
                     /locus_tag="VFB80_13685"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HZN34873.1"
                     /translation="MISALLAAPASADVVFYRLPTTRGDVVMLEGATTVNPGGTVTFS
                     HPRFGKIHFDLASTEIRKAPTVSAQFSRVLGRAGSDAEKRMEAAQWALRHGLLAQFYS
                     TIDKVLEANPRHPRALLVRQLKAQMDAPLADPSRQEQELRKLVPLESMKFKTSKHFLL
                     MHDTPDTLTKGKLTRADERLQLLETVYECFLLRFYAYGVALEVPKERLKVVLFNDYQQ
                     FRQFADKQSPALASASGFWDGETNTSVFFDHGTNDEFKALKQISDELQFRKEEAVRTR
                     AGNAANLVRTADTLKMLVEIERENCDIEVVSHETTHQMAGNTGLLPRHVRIPTWVHEG
                     LATYFETPDGATWGGIGEVNASRLSLYRDLEWDKTHSNIGFIVGDQIFDFARTTRGTL
                     HGYGQSWALTHFLLEKHFDKFMTFYRRLGELPPDTFLSSDVVNRLFDESLGLDRNVLD
                     SQWRLYMSNLKTDVELILEGQ"
BASE COUNT          651 a         1237 c         1109 g          608 t
ORIGIN      
        1 cggttgtctt gtccccacag acccagcgat cgcagttccg ccaggcaacc gtcgagcgcc
       61 gccgccactg cggcataact ggccgcttcg ctttcgcccg cggccagccg cgcggcgcag
      121 gcgtccagcg ccgcgacgag gtcggctgcg gcttgccgca cggtctggcg caccatcatc
      181 tccatcttca tccgcggttc gagtacgatg gacccttctg gcgcgtccgc cggcctccat
      241 cgtacgtccc catgccagtc accgtcgcct gtcgctgcgg ccagcggttc gccgctccca
      301 accacctcca aggaaagcag gtgccttgcc cggtctgcaa ggcgccaatt tcgattccgg
      361 cggcaggcat caagatcgcc tgccgctgcg gcatggtttc gttcgcgccc gatacgctcc
      421 gcggacagac gacgcgctgc cccggctgcg gcgacttgat ccgcgtcccc gcgctgggga
      481 ccgacgattt gcaggtagcc gggccgaagc ccgcacccga tccgctcggc cgcggaccgc
      541 ggatcgcccc gccggccaag agcccctggc tgatcgtcgg cctggtcgcc gggggctgcg
      601 gcctggtcgt ggtgatgatg atcgccgcgc tggcggtcat ggcgttgaag aactcgctcg
      661 gccaggcgcg gcggccgacc gttccgcccc cgggcttttc cacgccgccc gttgatcccg
      721 gtcccggcta ttcgcgcagt aatccgccgc ggccttggag cccgccgccc accccgccgc
      781 cctctgcgta tcaaccgcca ccgccgcccc ccacgagtat tcgacccgtg ccgtcgaatc
      841 cgtcgtttcc tcccttcggt ctgccgcctg cacccccgag cccacccagc agttccccga
      901 gcagcagcgc cagcagcccg ccgccggccg cgccgaagaa aaacgtgacg ctgcccgaag
      961 gcatcgcaac ctggtacgaa cagccgagcc ttaaactcac cggaatccgc cgtgtcggcc
     1021 cagcgtcgcc gccgtcggcc catttcagct ggatgacggg tttacttccg ttcctcgacc
     1081 acgacgagac gttcgacaag ctcaacttct cgcagccgct gacgcagcag gccaacttgc
     1141 aggtcggggc gacggtgatc ccgcagtttc tgaacccgct ggattcgcgg cagcggtgga
     1201 aaggctatcc gctcgacggc ctggcgctga cacacttcgt cggcatgtcg ggagtcgagg
     1261 acgcccgcaa tgtcgtcgcg gcgaagttgc cgcggagcga tccgcgggcc ggcgtcttcg
     1321 gctacgacga agtcgcccgc gccgagcaga ttaccgacgg ccagagccag acgatcatgg
     1381 tcgtcggcgg cggcgaactg gccaatccct ggctcttcgg cggcggggcg acgatccgcg
     1441 gcgcgcgcga gccgctgttc gacaagacca gcggcctggg caccaagggt ctggccggcg
     1501 gcggcacgct ggcgatcatg gccgacggct cggtgcggca gatcaacccc cagatcgacc
     1561 ccgccgtttt caaggcgatg tgtacgatcc acggcagcga gaaggtggac ctggagaaat
     1621 cggcgacgcc gtttgattgg gagctgctga agaagtgagc aaagccgcga ccagtggtcg
     1681 cggttcgcgg agcgatggtg agtggcgagt ggtcgcgcga caattggaga attgcaaaat
     1741 gcaaattgca aaatgcaaat tgatgaacga cctgcagggt cccaatatct tccgtgctcg
     1801 caacccaatt tccactttgc aattggcaat ttgcaatttg aaatgtctcg ggctggcatt
     1861 gatctccgcc cttcttgccg cccccgcctc ggccgacgtt gtcttctatc gcctgccgac
     1921 gacgcggggc gacgtggtga tgctggaagg ggcgacaacg gtcaaccccg gcgggacggt
     1981 caccttctcg cacccgcggt tcggaaaaat ccactttgat ctggcgagca cagagattcg
     2041 caaagcgccg accgtctcgg cccagttcag ccgcgtgctc ggccgggctg gcagcgacgc
     2101 ggaaaaacgg atggaggccg cccagtgggc cctgcggcac ggcctgctcg cgcagttcta
     2161 cagcacaatc gacaaggtcc tggaagccaa cccgcgccat ccgcgggcgc tcctcgtgcg
     2221 gcagctcaag gcgcaaatgg acgccccgct ggcggatcca tcgcggcaag agcaggagct
     2281 ccgcaagctc gtcccgctgg aaagcatgaa gttcaagacc agcaagcact tcctgctgat
     2341 gcacgacacg cccgacacgc tcaccaaggg caagctcacg cgggccgacg agcgattgca
     2401 actgctcgag accgtctacg agtgctttct gctgcgattc tatgcctacg gcgtggccct
     2461 ggaagtgccc aaggagcggc tgaaggtcgt gctgttcaac gactatcagc agttccggca
     2521 gttcgccgac aagcagagcc cggccctggc cagcgccagc ggcttttggg acggcgagac
     2581 caacaccagc gtgttcttcg accacggcac caacgacgaa ttcaaagccc tgaagcaaat
     2641 ctccgatgag ctgcagtttc ggaaggaaga agcggtccgc acccgggccg gcaatgcggc
     2701 caatctcgtc cgcaccgccg acacgctgaa gatgctggtc gagatcgaac gcgaaaactg
     2761 cgacatcgaa gtcgtcagcc acgagacgac gcaccagatg gccggcaaca ccggcctgtt
     2821 gccgcggcac gtgcggattc ccacctgggt ccacgagggg ctggccacct atttcgaaac
     2881 gcccgacggc gcgacctggg gcggcatcgg cgaggtcaac gcctcgcggc tctcgctcta
     2941 tcgcgacctg gagtgggaca agacgcattc caacatcggc ttcatcgtcg gcgaccagat
     3001 tttcgacttc gcccgcacga cccgcggcac gctgcacggc tacggccagt cctgggccct
     3061 gacgcacttc ctgctggaaa agcactttga caaattcatg accttttatc gccgcctcgg
     3121 cgaattgccg cccgacacgt tcctcagctc cgatgtcgtc aatcggctct tcgacgagtc
     3181 gctcggcctc gaccgcaacg tgctcgacag ccagtggcgg ctctacatga gcaacctcaa
     3241 gaccgacgtc gagctgatcc tggaagggca gtagccgggt cgtctcgttc ccaagctctg
     3301 ctagctctgc ttgggaacgc acgtctggaa gctccgcttc ctgaagacga aggactaaac
     3361 cgtgcagcgg gaggtgctca acaccgtcgg actgggattg tcgatgatgg gcggggtgct
     3421 attgacctcc ttctgctgca taaacccgct cgaccacacc gagacaggga tcgagcaggg
     3481 cgcaatgctc acgctggccg gagccttatg cggggcgttc gcctggaagt tcaggcagtg
     3541 gtggcggact ctcccaagac cggctaggcc agggaattgc actttgcaat tcgcaattca
     3601 ctttg
//
LOCUS       DASRJB010000527         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2684207, whole genome shotgun sequence.
ACCESSION   DASRJB010000527 DASRJB010000000
VERSION     DASRJB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684207"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="VFB80_13690"
     CDS             <1..1940
                     /locus_tag="VFB80_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN34874.1"
                     /translation="VHRMWAGPTGNASANRRRAGMLESMSATATPAPTAPAAAADQPA
                     IKKLSITAPRKDDREPDKRPLDLRLITRLIGYMRPYAGKRNAMIVMVLLRSLQLPAMA
                     WSIGAVIDGPIAHHAPLSSILWAAAGVLALAGSTQVVFHFRQRLALELGEAVIHDLRQ
                     QIYEHLQRMPMSFYNKTKIGRIISRVTSDCEALRVGVQDVLFVSLVGVGQMLTAGLIM
                     LYYDWALFSVVAAMSPVLYVLNRYFRRQLSAAYRRIQESFSRVTATLAESINGVRVTQ
                     AYVRQETNAGLFRELMQWHGNNVMQGVRMEGLLMPLLELNSQTFIAALLLVGGYRVLT
                     PEIGMPPGDLIRFFFLANIFFSPIQILGNQYNQALTAMAGAERVFGLLDRPPEWQDAP
                     DARPLPPIVGHVELRSVTFGYEADRMVLHDICFTAQPGQTVALVGRTGSGKSSIINLI
                     ARFYLPQQGAVLIDGHDTRGITGDSLHHQMGLVLQQNFLFTGTITENIRVGKPTATDA
                     EVLEAARRLDCLDMLQALPQGLETQVGERGASLSLGQRQLVCFARAMLADPRILILDE
                     ATSSVDTLTELRIQRALAKLLAGRTSFVVAHRLSTIRSADQILVLEDGRILQRGTHAE
                     LLAFGGEYAHLVEQFSRGVAA"
     gene            complement(2073..2786)
                     /locus_tag="VFB80_13695"
     CDS             complement(2073..2786)
                     /locus_tag="VFB80_13695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN34875.1"
                     /translation="MHYQHAEIEGRYRGLTDSPVAVDYAGRSFDDFDLREFLTACLGR
                     MRFAGREPRVLEYGTGTGPGACFLAARGFRVTAIDISPTAIGLARRYAAERGLAIDYA
                     VANICDYGGQSQAFDLVIDSFCLHRIIADEARQQAMRNVRRLLKPSGYYLLGSVILRP
                     GRDYGAERLDPATGVVYSPLASGAANFHDAVQQGGRWWYARYRLLSAARLREELEAAG
                     FIVREQPGEGGRILCVPAG"
     gene            complement(2741..3862)
                     /locus_tag="VFB80_13700"
     CDS             complement(2741..3862)
                     /locus_tag="VFB80_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34876.1"
                     /translation="MHDRSLAYAMKGALWRLPLAVCDWAANRACRGTRPRLLIVRHPA
                     KKARFYDVILDWVAQHVPEARGRFELRLLPCRLQAPGDYALHVPWLQDPVEAWSLAAY
                     RQASALARRCDQAGIPVFNRVDRLANASKRECSRRLAALGIRTPRVELIDDPARFRRT
                     QLGLSLPLIVREDQGHGGAMHRVDAAGDLARVPIEPMRRPVAIELIDVRCPADGLFRK
                     FRYVAAGSLGVPHHLQTTDHWITRGTIRVHTPAVDEQEIAFISQPEPHQALFQAARQA
                     LGLDLVAFDYGFDASGRLVVWEANPFPHFHLPRRRLSYLAPAMHRTLAAVVHAYLERA
                     GLPIPEPLAAILDSPAVSRSSALQGCTTNTPRSKGDIAA"
     gene            complement(3945..4592)
                     /locus_tag="VFB80_13705"
     CDS             complement(3945..4592)
                     /locus_tag="VFB80_13705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN34877.1"
                     /translation="MSEPLPDWIGRVLAHRDLRGQGHAQRQADGNLGLGWIYYGLARV
                     LRPQHVVVIGSLRGFSPLVFGRALADNGEGGLVTFLDPSLVDDFWRDAERVRRHFAAY
                     GLANIRHFPQTTQDFAQTAAFRDLPPVELLFVDGYHTAEQARFDHEAFTAKLTPAAIV
                     LFHDSTTLVTSGIYGEERRYLHTVKHYIDELKRNPTLQVFDLPVDSGVTLVRRAE"
     gene            complement(4589..5983)
                     /locus_tag="VFB80_13710"
     CDS             complement(4589..5983)
                     /locus_tag="VFB80_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HZN34878.1"
                     /translation="MDIGELLSRPDEVARLAAVLGDAARVSYAPPNIYPMAAPLLSAG
                     GDCLRRQPGERELGIYVHVPYCNYSCTFCFYATRLTPQPEEMQRYVRALTRELAWVTP
                     GMRLSQLYVGGGTPTTLPPALLDELLAAVCERMTPRGRDVHTVETSPESLTPEHAAVL
                     QARGIERVSMGIQSLSGGVLDVVKRRHAAEQALAACDLLVSAGFVVNVDLIYGLPGQT
                     HDSFRRDLETLAARGVQSITTYNLRVNERTPVGRSLEEHERLDLPALIRWRAMIRTTV
                     DSMGFEPLRWHTFRRKLTPETAATAAGRFLDLTGRGNQFGAGVSARSRLESCVYRNHP
                     SYDMYLARVESGENPVHETKVLSETERRLRYVALTLGDGHPLVLAEYSAEFGSEFADD
                     FSEAAGQLEAAGLIRDEGQRLVMTAAGELVYDLVTRAFYPNPVRRWLDERQQLAHTAA
                     NLHPRQRPREAAPL"
BASE COUNT         1026 a         2152 c         2187 g          982 t
ORIGIN      
        1 cagtccaccg aatgtgggcc ggccctaccg ggaatgcctc cgccaatcgc cgccgcgccg
       61 gtatgctgga aagcatgtcc gcgaccgcca cgcccgcacc aaccgccccg gcagccgcgg
      121 ccgatcagcc cgcgatcaag aagctctcga tcacggcccc gcggaaggat gaccgcgagc
      181 cggacaagcg gccgctggac ctgcggctga tcacccgcct gatcggctac atgcggccct
      241 acgcgggcaa gcggaacgcc atgatcgtga tggtgctgct gcggtcgctg cagctgcctg
      301 ccatggcctg gagcatcggc gcggtgatcg acggcccgat cgcccaccac gcgccgctga
      361 gcagcatcct gtgggccgcc gccggcgtgc tggccctggc cggcagcacg caagtcgtat
      421 ttcacttccg ccagcggctg gcgctggaat tgggcgaggc ggtaatccac gacctccgcc
      481 agcagatcta cgagcatctg cagcggatgc cgatgagctt ttacaacaag accaagatcg
      541 gccgcatcat cagccgcgtg acgagcgact gcgaggcctt gcgggtcggc gtgcaggacg
      601 tgctgttcgt gagcctggtg ggcgtggggc aaatgctgac ggccgggctg atcatgcttt
      661 attacgactg ggccctgttc agcgtcgtgg cggcgatgag cccggtgctg tacgtgctga
      721 accgctattt ccgccgccag ctttcggccg cctatcgccg gatccaggag agcttcagcc
      781 gggtgacggc gacgctggcc gagtcgatca acggggtccg cgtgacgcag gcctacgtgc
      841 ggcaggagac gaacgccggg ctgtttcgcg agctgatgca gtggcacggc aacaacgtga
      901 tgcagggcgt gcggatggaa ggcctgctga tgccgctgct ggagctcaac agccagacgt
      961 tcatcgcggc cctgttgctc gtgggcggct atcgcgtgct cacgccggag attggcatgc
     1021 cgccggggga cctgatccgc ttcttcttcc tggcgaacat ctttttcagc ccgatccaga
     1081 ttctcggcaa tcagtacaac caggcgctga cggccatggc cggcgcggag cgcgtgttcg
     1141 gcctcttgga ccggccgccc gaatggcagg acgcaccgga cgccaggccg ctgccgccga
     1201 tcgtggggca cgtcgagttg cggagcgtga ccttcggcta cgaagccgac cggatggtgc
     1261 tgcacgacat ctgcttcacc gcccagccgg gacagacggt agccctggtc ggccgcacgg
     1321 ggagcggcaa gagctcgatc atcaacctga tcgcccggtt ctatcttccg cagcagggcg
     1381 cggtgctgat cgatggccac gacacgcgcg gcatcaccgg cgactcgctg caccatcaga
     1441 tgggcctggt gctccagcag aatttcctgt tcaccggcac gatcaccgag aacatccggg
     1501 tcggcaagcc cacggccacg gacgccgagg tgctcgaagc agcccgccgg ctggattgcc
     1561 tcgacatgct gcaggcgctg ccgcagggcc tggaaacgca ggtgggcgag cggggcgcga
     1621 gcctgtcgct cggccagcgg caactggtct gcttcgcgcg ggcgatgctg gccgatcccc
     1681 ggattctgat cctcgatgag gcgaccagca gcgtcgatac gctcaccgag ctgcggattc
     1741 agcgggccct cgcgaaactg ctggccggcc gcaccagctt cgtggtcgcc caccgcctga
     1801 gcacgatccg cagcgccgac cagattctcg tgctcgagga cggccgcatc ctgcagcgcg
     1861 gcacgcacgc cgagctgctg gctttcggcg gcgaatatgc ccacctcgtc gagcaattct
     1921 cccgcggcgt cgcggcgtag ggtgtgacaa ggccgccgcg gccgcaggca caccatgacg
     1981 agcgcggacg attagcgttg cttcgatctc ggtgggccgg cgctcgccca ccgccaggcg
     2041 gcgttggccg atgctggtcc caccctacgc gcctagccag ccggcacgca cagaattcgg
     2101 ccgccttcgc cgggctgctc gcggacgatg aatccggcgg cctccagctc ctcccgcagt
     2161 cgcgcggccg atagcagccg ataccgcgcg taccaccagc ggccgccctg ctggacggcg
     2221 tcgtggaaat tcgccgcgcc gctcgcgagc ggcgagtaga caactccggt cgccggatcc
     2281 agccgctcgg caccgtagtc gcgtcccggg cgaaggatga ccgagccgag caggtaatag
     2341 ccgctgggtt tgagcagtcg gcgaacgttt cgcatggcct gctggcgggc ctcgtcggcg
     2401 atgatgcggt gcaggcagaa actgtcgatc accaggtcga aagcctggga ctggccgccg
     2461 tagtcgcaga tgttggccac cgcgtagtcg atggccaggc cgcgctcggc cgcgtagcgg
     2521 cgggccaggc cgatggccgt cggcgaaatg tcgatcgccg tcacgcgaaa gccgcgagcc
     2581 gccaggaagc acgcgccggg ccccgtgccc gtgccatatt ccagaacccg cggctcgcgc
     2641 ccggcaaacc gcatccggcc gaggcaagct gtcaggaatt cccgcaggtc gaagtcgtcg
     2701 aagctgcggc cggcgtaatc gaccgccacg ggcgaatcgg tcaggccgcg atatcgccct
     2761 tcgatctcgg cgtgttggta gtgcatcctt gcagggcgct ggaccgcgac acggccggcg
     2821 aatccaaaat tgccgccagc ggctcgggaa tcggcaggcc cgcccgctcc aggtaggcat
     2881 ggacgacggc cgccagcgtg cggtgcatcg cgggcgccag ataagatagc cgccgccgcg
     2941 gcagatgaaa atgcggaaac ggattggctt cccagaccac cagccggccg ctggcgtcaa
     3001 agccgtagtc gaacgccacc agatcgaggc ccagcgcttg ccgcgcggcc tgaaacagcg
     3061 cctggtgcgg ttccggctgg ctgatgaagg cgatctcctg ctcatccacg gcgggagtgt
     3121 ggacgcggat ggtaccccgc gtgatccagt ggtcggtcgt ttgcaggtgg tgcggcacgc
     3181 ccaggctgcc ggccgcgaca tagcggaact tgcggaacag gccgtcggcc ggacatcgca
     3241 cgtcgatcag ctcgatcgcc acgggccgcc gcatcggctc gatgggaacg cgggccagat
     3301 cgcccgccgc atcgacgcgg tgcatcgcgc cgccgtgtcc ctggtcctcg cgaacgatca
     3361 gcggcaacga caggcccagc tgcgtgcggc ggaacctggc aggatcatcg atgagctcga
     3421 cgcgcggcgt gcggatgccc agtgccgcca gtcggcggga gcattcgcgt ttggacgcat
     3481 tggccaggcg gtccacgcgg ttgaaaacgg gaatgcccgc ctggtcgcag cggcgagcca
     3541 gcgcggaggc ctgccgatag gccgccagcg accaggcttc gaccggatcc tgcagccagg
     3601 gcacgtgcag cgcgtaatcg ccgggagcct gcaggcggca gggcaacaat cgcagctcaa
     3661 agcggccgcg agcctcgggg acgtgctggg caacccagtc caagatcacg tcgtaaaagc
     3721 gggccttctt ggccgggtgc cgcacgatca aaagcctggg cctcgttccg cggcaagcac
     3781 ggttggcggc ccagtcgcac acagcgagcg gcaggcgcca cagggcgcct ttcatggcat
     3841 aggccaggct gcgatcgtgc atgcgggcac cctgcgagtg ctgagcaatt ctgccaacta
     3901 cgccctcgat cgacaatgtc gattccctcg gcccaactcc gcggtcactc ggctcgccgg
     3961 acgagcgtca cgccgctgtc cacgggcaga tcgaagacct gcagggtcgg attgcgcttc
     4021 agctcgtcga tgtagtgctt gacggtgtgc aagtagcggc gctcctcgcc gtaaatgccg
     4081 ctggtgacga gcgtcgtgct gtcgtggaac aggacgatgg cggcgggcgt cagcttggcg
     4141 gtgaaggcct cgtggtcgaa ccgcgcctgc tcggccgtat ggtagccatc gacgaacagc
     4201 agctcgaccg gcggcaagtc gcgaaacgcg gcggtttggg caaagtcctg cgtcgtctgc
     4261 ggaaagtggc gaatgttggc caggccgtag gcggcgaagt gcctgcggac tctttccgcg
     4321 tcccgccaga aatcatcgac cagcgacggg tcgaggaacg tgaccaggcc gccttcgccg
     4381 ttgtcggcca gggcccggcc gaaaaccagt ggcgaaaaac cgcgcagcga accgatcacg
     4441 accacgtgct gcggccgcag cacgcgggcc agcccgtagt aaatccagcc gaggcccaga
     4501 ttgccgtctg cctggcgctg agcatgtccc tgcccgcgga ggtcgcggtg agccagcacg
     4561 cggccaatcc agtcgggcag cggctcgctc atagcggcgc ggcctcgcgc gggcgctgcc
     4621 gcgggtgcag gttggcggcg gtgtgggcca gctgctgccg ctcgtcgagc caccgccgca
     4681 cgggattggg ataaaaggcc cgcgtcacca ggtcgtaaac gagctcgccg gccgcggtca
     4741 tgaccagccg ctggccctcg tcgcggatca gccccgccgc ctcgagctgg cccgcggcct
     4801 cgctgaagtc gtcggcgaat tcgctgccga attcggcgct gtattccgcc agcacgagcg
     4861 ggtgcccgtc ccccagcgtc agcgcgacgt agcgcagccg ccgttcggtt tcgctcagca
     4921 ccttggtctc gtggacggga ttctcgccgc tttcgacccg cgccagatac atgtcgtagc
     4981 tcggatggtt gcggtacaca cagctctcca gccgcgagcg tgcgctgaca cccgcgccga
     5041 actggttgcc gcgccccgtc aagtccagga agcggcccgc ggcggtggcc gcagtctcgg
     5101 gggtgagctt gcggcgaaac gtgtgccacc gcagcggctc aaagcccatg ctgtccaccg
     5161 tggtccggat catcgcccgc caccggatga gcgcaggcag gtcgagccgc tcgtgttcct
     5221 cgagcgaccg gccgacgggc gtccgctcgt tgacgcgcag gttgtaggtc gtgatcgatt
     5281 ggacgccgcg ggcggcgagc gtttccaggt cgcggcgaaa cgagtcgtgc gtctggccgg
     5341 gcaggccgta aatcaagtcg acgttgacga cgaagcccgc gctcacgagc aggtcgcagg
     5401 ccgccagcgc ctgttctgcc gcgtggcggc gcttgacgac gtccagcact ccgccgctca
     5461 gcgactgaat gcccatgctc acccgttcga tgccgcgggc ttggagcacg gccgcgtgct
     5521 cgggcgtcag cgactcggga gaggtttcca cggtgtgcac gtcgcggccc cgcggcgtca
     5581 ttcgctcgca gacggcggcc agcaattcgt cgagcagcgc cggcggcagc gtcgtcggcg
     5641 ttcccccgcc gacatagagc tggctcagcc gcatgccagg cgtcacccag gcgagttcgc
     5701 gggtcagcgc ccgcacgtac cgctgcattt cttccggctg cggcgtcagg cgcgtggcgt
     5761 agaagcaaaa cgtgcaactg tagttgcagt acggcacgtg gacgtagatg cccagctcgc
     5821 gctcgcccgg ctgccgccgc aggcagtcgc cgccggccga gagcagcggg gcggccatcg
     5881 gatagatgtt cggcggggcg tacgagacgc gggccgcgtc tccgagcacg gctgccaggc
     5941 gagcgacttc gtcgggcctg ctaagcagtt cgccgatgtc catgggatcc atcctccgga
     6001 agtgccaccg tggccgggcg gccgcattgt agcggcgagg caggatttcg gtaatcgaga
     6061 ctcgctggcg gatctctcgg ggcggagtcg gttcggccga atcgccaatc aaacgtcgat
     6121 tgtgttatcg ccgcgatgcg ttctataatc cccgccctgg acggagccga cttgtgatga
     6181 caatccgccg gacgcggccg cctcccacgc tgatcgccac cgccatttcc ctcggatacg
     6241 gcccggcggc caagctgctt gtcctgacct ctggcctgcg gcagcgcggc gtgcgggtgg
     6301 tgttcgtggg aaacggcagc gcgctggagc tcgcttcccg ccacggc
//
LOCUS       DASRJB010000528         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3528320, whole genome shotgun sequence.
ACCESSION   DASRJB010000528 DASRJB010000000
VERSION     DASRJB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528320"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFB80_13715"
     CDS             <1..1045
                     /locus_tag="VFB80_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34879.1"
                     /translation="YLALTGVYHARRSGNPPPTPNDIPTYGAVLRRISKQPRFVSDAI
                     HVNGPALVPREAGPGQNGGFLGRAFEPLVVGDPNSAAGAIPGLAAQADLPIVRQGDRL
                     TLKQTLDRYAQRLEADPRAADMSRQYEQAYALLASAAAREAFDLEAEPAAVRDRYGRH
                     RSGQALLLARRLVEAGVPYINVIWNHSNRGQDDAPDDTDLYGWDTHNDIFTALKDRLL
                     PRFDESFSALIADLDQRGLLDQTLVVCMGEFGRAPRVALEPKFAGATPGRKHWASVYS
                     IVVAGAGASRGAVLGASDRMGAEPITDRYGPWDVAATMYHALGIDPRGHYTDSLGRPY
                     LITTGQPIAGLYA"
     gene            1156..2145
                     /locus_tag="VFB80_13720"
     CDS             1156..2145
                     /locus_tag="VFB80_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZN34880.1"
                     /translation="MSDYYDTLGVGRGATSDEIQKAYRKLAKKYHPDLNPDDKAAQKK
                     FKEVQQAYDVLSDEKKRKMYDQFGPAFEQIQQGGGPQWSGQMPEGFDFSQMFGGGPRG
                     GRAAGGAGPLPPDLEELLKQFTGGGGGFEFGGGGGRRRGRRQPAQPGADVQHEVEVPF
                     RTAVTGGEVNLRLRRPGSKTETLSVKIPAGIEDGQTIRLRGQGEESPHDGLAGDLLIT
                     VRVAPHPSFRRDGLDLIVRVPVTLAEAALGAKVDVPTPQGEIALKVPPGTSSGTRLRA
                     KGQGIRKADGRAGDLYAEILIAIPRQIEDEAAELIRKLDSHLRLDNPRAELEW"
     gene            2153..2470
                     /locus_tag="VFB80_13725"
     CDS             2153..2470
                     /locus_tag="VFB80_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34881.1"
                     /translation="MPAHWSKRLSIAAASVLVLASTAWAELPPPLIKRIDGATRARVL
                     AALAGLIILGFAMVLLTWLGARVVQRYRQGSSVFRPTPRPSEHDWAKTPLVPGEDDPP
                     ADP"
     gene            complement(2474..2920)
                     /locus_tag="VFB80_13730"
     CDS             complement(2474..2920)
                     /locus_tag="VFB80_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34882.1"
                     /translation="MSFDPQGGRSPYIPPMNDPYAPRKSSGASMVLWVVVGGGGLLLV
                     LLCCGGGAGLLVFTRNFMQAEVKDQLRDNPKLREHIGELETVELDFAGTMAVDDDETF
                     RYNVRGSKGSGELTVVESPDDSTIVEATLRLKDGTQVKIVPLAPEG"
     gene            3061..3681
                     /locus_tag="VFB80_13735"
     CDS             3061..3681
                     /locus_tag="VFB80_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34883.1"
                     /translation="MSYNSHKCLFGLAAAILLAGCSESVQTPTSYKKWNAKDGLFAVE
                     YPEGWKADGGGKQGIQWAEFKKGSCIITIDTNTSGSVVTDIAASGLGGGDDGVPIDPK
                     VAEKLAPAAAAHNWIAKDPQRFEKFKNYKEEEPTKITPPIGEGRKSLFTASAGMGQKV
                     KGYRVTIPTNTKAIIVFAYGAEKDWSKVQGAYDKIFDGMEMGTPEM"
     gene            3868..>4131
                     /locus_tag="VFB80_13740"
     CDS             3868..>4131
                     /locus_tag="VFB80_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZN34884.1"
                     /translation="MGFPIYYNLQMLLFLSPALILAFIAQMWVQSAYARGRQMAASVS
                     GFMAARRLLDSAGLQQVEIETVPGQLSDHYDPRGKVLRLSEENY"
BASE COUNT          728 a         1397 c         1378 g          628 t
ORIGIN      
        1 ctacctcgcc ctgactggtg tctatcacgc ccgccgctcg ggcaacccgc cgccgactcc
       61 caacgacatt ccgacgtacg gcgcggtcct gcgccgcatt tcgaagcagc cgcggtttgt
      121 ctcggacgcg atccacgtca acggaccggc gctggtgccg cgcgaagcgg ggccgggcca
      181 gaatggcggc ttcctgggcc gggcctttga gccgctggtg gtcggcgatc caaactcggc
      241 ggcgggggcg attcccgggc tcgcagcgca ggccgacctg ccgatcgtcc gtcagggcga
      301 ccgcctgacg ctcaagcaga cgctcgaccg ctatgcccag cggctggagg ctgacccgcg
      361 ggccgccgac atgagccggc agtacgagca ggcgtatgcc ctgctggcct cggccgcggc
      421 ccgcgaggcg ttcgacctgg aggccgagcc ggcggccgtc cgcgaccgct atggccggca
      481 ccgctcgggg caggcgctgc tcctggcccg ccggctggtc gaagcgggcg tgccctacat
      541 caacgtcatc tggaaccaca gcaaccgcgg ccaggacgac gcccccgacg acaccgacct
      601 ctacggctgg gacacgcaca acgacatctt cacggcgctc aaggaccgcc tgttgccgcg
      661 gttcgacgag agtttttcag ccctgattgc cgacctcgac cagcggggcc tcctggatca
      721 gacgctggtc gtctgcatgg gcgagttcgg ccgggccccg cgggtcgcgc tggagccgaa
      781 gttcgccggg gccacgccag gccgcaagca ctgggccagc gtctattcga tcgtcgtcgc
      841 cggggccggc gccagtcgcg gggcggtgct cggtgcctcg gaccggatgg gcgcggagcc
      901 gatcaccgac cgctacggcc cctgggacgt cgcggccacg atgtaccacg ccctgggcat
      961 cgatccccgc ggccactaca ccgattcgct ggggcggccg tatctcatca ccaccggcca
     1021 gccgatcgcg gggctgtacg cgtagctagt ccaagccgcg accggtctac caatgcaccg
     1081 cgaccactgg tcgcggcttt gggcaaaggg ctccgccact ggccgtcttg gccaccggct
     1141 tcgtagaatc gccacatgag cgattattac gacacgctgg gcgtcggccg gggagccacc
     1201 agcgacgaga ttcaaaaggc ctaccgcaag ctggcgaaga aatatcaccc ggacctgaac
     1261 cccgacgaca aggccgcgca gaagaagttc aaggaagtgc agcaagccta cgacgtgctg
     1321 agcgacgaga agaaaaggaa gatgtacgac cagttcgggc cggccttcga acagatccag
     1381 caaggcggcg gcccgcagtg gtcgggccag atgccggagg gctttgactt cagccagatg
     1441 ttcggcggcg gcccgcgcgg cggacgagcg gccggcggcg ctggcccgct gcccccggac
     1501 ctggaagaac tgctcaagca attcacgggg ggcggaggcg gatttgaatt cggcggcggt
     1561 ggcggccgcc gacgcggccg gcgtcagccc gcacagcccg gggccgacgt gcagcacgaa
     1621 gtcgaagtcc ccttccgcac ggcggtcacc ggcggcgaag tgaacctgcg gctgcggcgg
     1681 ccaggcagta agaccgagac gctttcggtg aagattcccg cggggatcga ggacggccag
     1741 acgatccgcc tccgcggtca gggcgaggaa tcgccccatg acggtctggc cggcgacctg
     1801 ctgatcaccg tccgcgtcgc cccacacccg tcgttccgcc gcgacggcct ggacctgatc
     1861 gtccgcgtgc cggtgacgct ggccgaagcc gccctgggcg cgaaggtcga tgttcccacc
     1921 ccgcagggcg agatcgccct caaggttccg ccgggaactt ccagcggcac gcgcttgcgg
     1981 gccaagggcc agggcattcg caaggccgac ggcagggccg gcgacctgta cgccgagatt
     2041 ctgatcgcga ttcccaggca gatcgaagac gaagccgccg agctgattcg caagctggac
     2101 tcccacctgc ggctcgacaa cccgcgggcg gaactggagt ggtgagacac cgatgccagc
     2161 ccactggtcc aagcgactct cgatcgccgc ggcaagcgtg ctcgtcctgg cgagcacggc
     2221 ctgggccgag ctgcctccgc cgctcatcaa gcggatcgac ggggcgacgc gggcccgtgt
     2281 gctggcggcc ctggcggggc tgatcatcct gggcttcgcc atggtgctgc tcacctggct
     2341 cggggcgcgg gtggtgcagc gctaccgcca gggctcgtcc gtgtttcgcc ccacgccgcg
     2401 gccaagcgag cacgactggg ccaagacgcc gctggtgccc ggcgaggacg atccgccggc
     2461 tgatccgtga ctctcagccc tccggggcta gcggcacaat tttcacctgc gtgccgtcct
     2521 tgaggcggag cgtggcctcg acgatggtgc tgtcgtcggg cgactcgacg actgtcaact
     2581 cgcccgagcc cttgctgccg cggacgttgt agcgaaatgt ctcatcatca tcgacggcca
     2641 tggtgcccgc gaagtcgagc tccaccgttt ccagctcacc gatgtgctcc cggagcttgg
     2701 gattgtcgcg gagctggtcc ttgacctcgg cctgcatgaa gttccgcgtg aacacgagca
     2761 gcccggcgcc gccgccgcag cagagcagca cgagcaacaa gccgccgccc cccaccacga
     2821 cccacagcac catcgaggcc ccgctggact tccgcggagc atacggatcg ttcatcggcg
     2881 gaatgtaggg cgagcggccg ccttgcggat cgaagctcat ttgcggctcc tggctggaag
     2941 tgtggaatat cgtaccgcac cggcggttct aagccagcag cgacttgtcc ggcgcgcgtc
     3001 tcgattacgc gccctgttag tgctaaccta gcacttcgtc cattttccag gagccctgat
     3061 atgtcctaca actcgcacaa gtgtttgttt ggtctggcgg ccgcgatcct gctggccggg
     3121 tgcagcgaat cggtgcaaac gccgacctcg tacaagaagt ggaacgccaa ggacggactg
     3181 ttcgccgtcg agtaccccga aggctggaaa gccgacggcg gcggcaagca gggcatccag
     3241 tgggcggagt tcaaaaaggg gagttgcatt atcacgatcg acacgaacac cagcgggtcc
     3301 gtcgtcaccg acatcgccgc gtcgggcctc ggcggaggcg acgacggcgt tccgatcgat
     3361 cccaaagtgg ccgaaaagct cgccccggca gccgcggccc ataactggat cgccaaggac
     3421 ccgcagcggt tcgagaagtt caagaactac aaggaggagg agccgaccaa gatcacgccg
     3481 ccgatcggcg aggggcgcaa gagcctgttc acggcgtcgg ccggcatggg tcagaaggtg
     3541 aagggctacc gggtcacgat tccgacgaac accaaggcga tcatcgtctt cgcctacggc
     3601 gccgaaaagg actggagcaa ggtccaggga gcctacgaca agatctttga cggcatggag
     3661 atgggtacgc cagagatgta ggcgccggtt ggtcggtcag tcggtaaatc ggtcggtcgg
     3721 ttcgcgtggt gcaacgaccg actcaccgac tcaccgacta ccgaccaccg accaacctgc
     3781 cccaaaatcg gcgcggggtt tgcaccaatc cggtgtgact gtcatttcgc gccacaagat
     3841 cgcgccggcc accggcgaat taccgatatg ggatttccga tctattacaa cctgcaaatg
     3901 ctgctgtttc tctcgccggc cctaattctg gcattcattg cccagatgtg ggtccagtcg
     3961 gcgtacgccc gcggccggca gatggccgcc agcgtgagcg ggttcatggc ggcccgccgg
     4021 ctgctcgatt ccgccggctt gcagcaagtc gagatcgaga ccgttccggg ccagctctcg
     4081 gaccactacg acccgcgcgg gaaggtgctg cgcctgagcg aagagaacta t
//
LOCUS       DASRJB010000529         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_145943, whole genome shotgun sequence.
ACCESSION   DASRJB010000529 DASRJB010000000
VERSION     DASRJB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145943"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..950)
                     /locus_tag="VFB80_13745"
     CDS             complement(27..950)
                     /locus_tag="VFB80_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZN34885.1"
                     /translation="MSGARQTTGVAGLDELLGGGLIPGTLTVVVGATGIGKTQLGLQF
                     ANAGLAQDGRRGIVLDCSSRGDSQSHAQYAQRMFGWQLAAADAGQPVDVESVFQSRDQ
                     WSRSADTPRPLVAALCDYLHIFDYHGRRVTRRDLEWEEWRNWQAELNARLRAAIAFLY
                     GNFVRGVRRVVIDGIEPADRPGDSIQFSLFEYVYHQILRKDPEWVARDLFRESYRRHA
                     AEAAQHTYDPARIGCLLLYTSHETMLDDLISRRLDEGDVLSNANTLIYIGKTRDGNRL
                     GRGLYIAKHRGSAAAERIVEFRIDDQGLSIA"
     gene            complement(947..2305)
                     /locus_tag="VFB80_13750"
     CDS             complement(947..2305)
                     /locus_tag="VFB80_13750"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HZN34886.1"
                     /translation="MACSRWLPGLLLLVLLPGMALARAPLTGALAEYVAAKDDSYRWV
                     KRSEGSVLTCKYIELTLTSQTWRGIVWKHQLFVVKPGRVNPEAKHATLFIAGGSWRDE
                     LADPKTQIKLPGEALALAAVAEGLQTPVAILLHVPQQPLFGGKREDEIISLTFREYLK
                     TGEADWPLLEPMVKSAVRAMDATQESLKQEWGLEVERFTITGASKRGWTTWLTAAADD
                     RVVAIAPMVINMLNMSPHSKLQKASFGGQASEQIDEYKGLDDYMDTPRGVALRKIVDP
                     WEYRSRLELPKLVILGTNDRYWPLDASNLYWNDLVGEKYLLWVPNAGHDLKERARVVA
                     GLAALNRRIISGQPLPKIDWKYASGSGGVRLSVTSDVKPSRVRIWTAKARARDFRESL
                     WTAIDAAAAEGGYAYQLAAPSAGYAALLGELVYNEGTETEFSLSTDVRMVPAATAAAG
                     GQ"
     gene            complement(2502..>2941)
                     /locus_tag="VFB80_13755"
     CDS             complement(2502..>2941)
                     /locus_tag="VFB80_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZN34887.1"
                     /translation="DAAPLAKLTAMGSLDLSNNQIKDMAPLAGLTKMHALYLTANPVA
                     DFAPLASLKNLQRLDLRKTGISDLTPLAGHTEWTFLFLSNNKIMEIAPLLAAVKKDLE
                     GQKRFAPFFKLYIGDNPLSDAGKAQLEELKKLVPSGRIHLTYP"
BASE COUNT          490 a          985 c          932 g          534 t
ORIGIN      
        1 ggtgtccgcc cggcgtggac gccgggctaa gcgatggaaa gcccctggtc gtcgatccgg
       61 aattcgacga tccgctcggc ggcggcgctg ccgcggtgct tggcgatgta caggccgcgg
      121 ccgaggcgat tgccgtcgcg agtcttgccg atatagatca gcgtgttggc gttcgagagc
      181 acgtccccct cgtccagccg ccggctgatc aggtcgtcga gcatcgtctc gtgcgaggtg
      241 tacagcagca ggcagccgat gcgggccgga tcgtacgtgt gctgggccgc ctctgctgcg
      301 tgccggcgat agctttcgcg gaacaagtcg cgggccaccc actccgggtc cttgcgcagg
      361 atctggtgat agacgtattc gaacaggctg aactggatcg aatcccccgg tcggtctgcc
      421 ggctcgatgc cgtcgatcac gacccgccgc acgccacgga cgaagttgcc gtacaggaag
      481 gcaatcgccg cccgcaaccg ggcgttcaat tccgcctgcc agttccgcca ctcctcccac
      541 tccaagtcac gccgcgtcac ccgccggccg tgatagtcga agatgtggag gtaatcacac
      601 aaagccgcga ccagtggtcg cggtgtatcg gcagaccgcg accactggtc gcggctttgg
      661 aacacgctct ccacgtccac cggctgcccg gcatcggcgg cggccagctg ccagccgaac
      721 atccgctgag cgtactgggc gtggctctgc gagtcgccgc ggctgctgca atcgagcaca
      781 atgccgcggc ggccgtcctg cgcgaggccc gcattcgcaa attgcagccc gagttgcgtc
      841 ttgccgattc ccgtcgcacc gacgaccacg gtcagcgtgc ccggaatcag cccgccaccg
      901 agcagttcgt cgagcccggc gacgcccgtc gtttgccgcg cgccgctcat tgcccgcctg
      961 ctgccgcggt cgccgccggc accatccgca catcggtgct gagtgagaac tcggtttccg
     1021 tcccttcgtt gtaaacgagc tcgccgagca gcgccgcata gcccgcactc ggagcggcga
     1081 gctgatacgc gtagcccccc tcggccgccg cagcatcgat tgcggtccag agcgactcgc
     1141 ggaagtcgcg cgcccgcgcc ttggcggtcc agatgcgaac ccgcgacggc ttgacgtcgc
     1201 tcgtcacgct cagtcgcact ccgccgctgc cgctagcgta tttccagtcg atcttgggca
     1261 gcggctggcc gctgatgatc cgccgattga gcgccgccag gccggcaacg acccgcgccc
     1321 gctccttcag gtcgtgaccc gcgttgggca cccatagcag gtatttctcg ccgaccaggt
     1381 cattccagta gaggttgctc gcgtcgagcg gccagtagcg gtcgttagtg cccaggatca
     1441 ccagcttggg cagctccagc cgcgagcggt actcccacgg atcgacgatc ttccgcagcg
     1501 ccacgccgcg gggagtatcc atgtaatcgt cgaggccctt gtattcgtcg atctgctcgg
     1561 aagcctggcc gccgaacgag gccttctgca gcttgctgtg cggagacatg ttgagcatgt
     1621 tgatgaccat cggggcgatc gccacgacgc ggtcgtcggc tgccgcggta agccaggtcg
     1681 tccagccgcg cttgctcgcg ccggtgatcg tgaaccgctc gacctccagg ccccactcct
     1741 gcttcagcga ttcctgcgtg gcgtccatcg cccggaccgc gctcttgacc atcggctcca
     1801 aaagcggcca gtcggcctcg cccgtcttca aatattcgcg gaacgtgagc gagatgatct
     1861 cgtcctcgcg cttgccgcca aacagcggct gctgcggcac gtgcagcagg atcgcgacgg
     1921 gtgtttgcag gccttcggcg accgcggcca gggccagggc ctcgccgggc agcttgatct
     1981 gcgtcttggg atctgccagc tcgtcgcgcc agctgccgcc ggcgatgaac agcgtcgcgt
     2041 gtttcgcctc cggattgacg cggcccggct tcacgacgaa gagttgatgc ttccagacga
     2101 tcccgcgcca ggtctgtgag gtcagcgtca gctcgatgta cttgcaggtg agcaccgagc
     2161 cttcgctgcg cttgacccag cggtaactgt cgtccttggc ggcgacgtat tccgcgagcg
     2221 ctccggtgag cggcgctcgg gccagggcca ttcccggcaa cagaaccagc agcagcaaac
     2281 ctgggagcca gcgcgagcaa gccatggaaa cctcgtaggc aaacttggaa cgaacaagga
     2341 gtcgaacaga agcaaacgaa gagaacgaag aaacgagaag cagatcggga tcaaagagga
     2401 ccgatcccat tttctccttc gttgccttcg tttccttctg ttcaaattgc ctgccgaatc
     2461 cgggcgaatt cggctcgccg atcctatgcc agacgccggg gctaaggata ggtcaaatga
     2521 atgcggccgc tgggaaccag cttcttcagc tcttcgagct gggccttgcc ggcgtcggag
     2581 agcgggttgt cgccgatata gagcttgaag aacggggcga accgcttctg cccctccagg
     2641 tccttcttca ccgcggcgag gagcggcgcg atctccatga tcttgttgtt gctcagaaac
     2701 agaaacgtcc actcggtgtg tccggcgagc ggcgtcaggt cgctgattcc cgttttccgc
     2761 agatccagcc gctgcaagtt cttcagactg gccagcggtg caaaatcggc gaccgggttg
     2821 gccgtcaggt acagggcgtg catcttcgtc agcccggcga gcggcgccat gtccttgatc
     2881 tgattgttcg acagatccag cgagcccatc gcggtcagct tggccagcgg cgcggcgtcg
     2941 g
//
LOCUS       DASRJB010000530         6354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_145975, whole genome shotgun sequence.
ACCESSION   DASRJB010000530 DASRJB010000000
VERSION     DASRJB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145975"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFB80_13760"
     CDS             <1..620
                     /locus_tag="VFB80_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34888.1"
                     /translation="GGLTTGLIGERAMGYHTFHHLENVPDVPQLKGMGKGGQLSRPAD
                     CEWYLQLLEELIAASKEPFPEARERARAAEMKLKQRAGAKNPLEKMKYMVTLMILPAT
                     GAAFDASGRTLAHRDVLRMAVAAERHRLATGAFPAQASDLVPKYLPAIPRDPFDGLPL
                     RLKPGADSGELFIYSVGKDGQDNGGLATSPSDPDIVVRLRAKKGP"
     gene            complement(689..1087)
                     /locus_tag="VFB80_13765"
     CDS             complement(689..1087)
                     /locus_tag="VFB80_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34889.1"
                     /translation="MRVYLLAAAALLLAAGTGCRNINLAKGGCGQCGTGGGCGPCDGG
                     GMGGNGGHGGHGGGAYAGYPGAGPHGHIPGGHYGPHGGPYGTPGYPHHHFDREYVGPP
                     GPPTAQVAYPYYTIRGPRDFLVDNPPSIGR"
     gene            1335..2228
                     /locus_tag="VFB80_13770"
     CDS             1335..2228
                     /locus_tag="VFB80_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34890.1"
                     /translation="MPTHPDLVCEVLDLCRQAAEANRSCAVRQGNVVSLTPGNAEDVM
                     IVADLHGNRLNFEKLLRVADLADNPRRHLVMQEVCHGGPEYPGENGGCMSHLLLEDCI
                     RLKTEFPERFHFLLSNHELAELGDFPICKSRKMLNLLFRSGINEMYGQAGEKVRDAYL
                     AFLATCPLAVRLSSGVFISHSLPDQCDREPFDASVLARPLSCADYRSGSPAFKLVWGR
                     DFRSANAEAFARQVGAELLIHGHEPCESGFSAPNARQVILDGCCSHATYLILPVGPKL
                     SQADVVARITSLHNRRETCPV"
     gene            2444..2677
                     /locus_tag="VFB80_13775"
     CDS             2444..2677
                     /locus_tag="VFB80_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34891.1"
                     /translation="MNPPVKQPKRPAFQFSLLGLMVVMFVVAAAAAPGYYMLRGQTLP
                     QSRLVGMLMVLAGPLLLMTLISVVLSLTHRGQR"
     gene            2879..3952
                     /locus_tag="VFB80_13780"
     CDS             2879..3952
                     /locus_tag="VFB80_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34892.1"
                     /translation="MQPPATHARLRAAGVEPTLEGVRDYLESLLDPQLEKQVDRWIAE
                     LGDPQFEVRERASLQLASLDFVPTLKLQAAATSPDPERAWRAKVALKGASPEAGLLPA
                     ALRLIEEEKLPVGLPLLVQLRSRIVSPAMQEAAARAMLAIVAEADRAALAELQRHEDA
                     AIRGVAGRLLARLDRPPAKYQEGSFDAVKLTPGGISGGGRNLIEGWEFRPKADIVVTH
                     LGLFDSGGDGLAAQHEIAIWDIEDPSGPLTQETMVPGTTAPLAGAFRLITTHPIKLSA
                     GRRYAIVAHYPDARDSNVGMINPAGLTIDYAEHVEALGRRYSFPHPSMAFPAEFAEGP
                     KHSKHGPTFRYSLAADPKDERKP"
     gene            3981..4577
                     /locus_tag="VFB80_13785"
     CDS             3981..4577
                     /locus_tag="VFB80_13785"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN34893.1"
                     /translation="MIALALTAALAALPAFAADLPAEERDAGFVELFNGKDFTGWRFG
                     DGPSPPAEIPANWRVEEGVIKVTGGGAPHLGSEKAYGNFELRLEWRGLKNGYNSGLYV
                     RSGKKVGSNQINLAHKSEGAFIGGKVTGAKAVGDLQKPAGEWNEWRVTAVGDKLTLHC
                     NGKLAWEATGFAPAEGHIGMQAEGAAMEFRNIRLREIK"
     gene            4592..5083
                     /locus_tag="VFB80_13790"
     CDS             4592..5083
                     /locus_tag="VFB80_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34894.1"
                     /translation="MQFSVAKLLGSTAFVAIGLAALASASHLLASAFLSVTILALCAA
                     VMGAIVTSGAARAFWLAFLLFAGVHLLLTLGPWLDDVTGEILLSRLAIDALGRAMGQD
                     VADHQRMPGVWLNLPYATAGSSYGFLNFVVIGQCWFSLALGCVAGELAGFFAGRASGE
                     RRR"
     gene            complement(5090..6175)
                     /locus_tag="VFB80_13795"
     CDS             complement(5090..6175)
                     /locus_tag="VFB80_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN34895.1"
                     /translation="MASRRLEPIWQLTLARVKEFTRQPAAIFWVYGFPILMMMSLGIA
                     FRDNPTEIIAVDLIVDQSRDQWSRSATPPAALSEIQSRLAADSRFKLNIQPADWQKRL
                     QAGKTDLVIEVLPTASQPAYRLWDEPHRAESRVARYAVEALLLRTDESPPPAPVQHLQ
                     KAGSRYIDFLLPGMIGMGLMGGGMWGVGFVIVDMRVRKLLKRFLATPMRRSDFLLAVM
                     ISRLMFSLADIVILLVFGYFVFGVRCQGSYLDLTAAVLVGGVAFSGIGLLVASRAETL
                     ETVSGLMNLIMLPMWILSGVFFSSERFPEVVQPLINLLPLTALNQLLRGIILEGKSLA
                     ALWQQAAILAAYGATTFAIALRIFRWK"
     gene            complement(6162..>6354)
                     /locus_tag="VFB80_13800"
     CDS             complement(6162..>6354)
                     /locus_tag="VFB80_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34896.1"
                     /translation="ARSQDESYSLAVTQPHVALPALLALLQSRGLALAHLTTRHATLE
                     DVFVSLTGRHLEEQPNGQP"
BASE COUNT         1086 a         2123 c         2109 g         1036 t
ORIGIN      
        1 agggcggtct gaccacgggg ctcatcggcg agcgggcgat gggctaccac acgttccatc
       61 acttggaaaa cgtgcccgac gttccgcagc tcaaaggaat gggaaaaggc gggcagctca
      121 gcaggccggc cgattgcgag tggtatctcc aactgctgga ggaactgatc gccgcgtcga
      181 aagagccctt tccggaagcc cgcgaacgcg cccgggcggc cgagatgaag ctcaagcagc
      241 gggccggcgc gaagaacccg ctggagaaaa tgaagtacat ggtgacgctg atgatcctgc
      301 cggcgaccgg cgcggcgttc gacgccagcg gccggacgct cgctcaccgc gacgtcctgc
      361 ggatggccgt cgcagccgag cgccatcgcc tggcgactgg cgccttcccc gcacaggcca
      421 gcgacctcgt gcccaagtac ctgcctgcga ttccgcgcga tcccttcgac ggcctgccgc
      481 tgcggctcaa gccgggggcg gattccggcg agctgttcat ctacagcgtc ggaaaggacg
      541 gccaggataa cggcgggctg gcgacttcgc ccagcgatcc cgacatcgtc gttcgcctgc
      601 gagcgaagaa ggggccgtag acgacgctgc cagcgtcgtc ctgacggcct cggccagcgc
      661 cgacgctggc agcgtcggct acgccaggct accggccgat gctcggcggg ttgtcgacca
      721 ggaagtcgcg cgggccgcgg atcgtgtagt acggataggc gacctgcgcc gtcggcggac
      781 caggcgggcc gacgtattcg cgatcgaagt ggtggtgcgg gtagccgggc gtgccatagg
      841 gaccgccgtg cgggccgtag tgtccgccgg gaatatggcc atgcggacca gcgccgggat
      901 agcccgcata cgcaccgccg ccgtgaccgc catgtccacc gttcccgccc atgccgcctc
      961 cgtcgcacgg cccgcagccg ccgccggtac cacattgccc acagccacct ttggccaggt
     1021 tgatgttgcg acagccggtg ccggcggcca acaacagcgc agccgcggcc agcaggtaaa
     1081 ccctcatggc aatgcatcct tccttggaat gggtcaacct cgcccggaga atgggtcgtt
     1141 tgcgtcgggc gcaggctgca agcggggccc aactggaccg ccgccctcct acaggcatca
     1201 tcggcggcaa aacccgcaga attcagaaaa tcggcatgcg cggcagcccc cgccaggcga
     1261 ctccagttgg tcttaggccc ggcgactgca tatatttgac tggagcgctg gtgctgaagg
     1321 cttccctcct cgcgatgccc acccatcccg atctcgtttg cgaagtgctc gacctctgcc
     1381 gccaggcggc cgaggcgaat cggtcctgcg cggtgcggca gggcaacgtc gtcagcctga
     1441 cgccgggcaa cgccgaagac gtgatgatcg ttgccgatct gcacggcaac cggctgaact
     1501 tcgagaagct gctccgcgtc gccgacctgg ccgacaatcc gcggcggcac ctggtcatgc
     1561 aggaggtctg ccacggcggc cccgagtatc cgggcgaaaa cggcggctgc atgtcgcacc
     1621 tcctgctcga ggactgcatc cgcctcaaga ccgaattccc ggagcggttc cacttcctgc
     1681 tctccaatca cgagctggcc gagctgggcg actttccgat ttgcaagtcg cggaagatgc
     1741 tcaacctgct cttccgcagc ggcatcaacg agatgtacgg ccaggccggc gagaaagtcc
     1801 gcgacgccta cctcgcgttc ctcgcgacct gcccgctggc ggtgcggctc tccagcggcg
     1861 ttttcatcag ccacagcctg cccgaccagt gcgaccgcga gccattcgac gccagcgtgc
     1921 tcgcgcggcc gctctcgtgt gccgactatc gcagcggcag cccggccttc aagctcgtct
     1981 ggggccgcga cttccgctcg gccaatgccg aagctttcgc ccgccaggtc ggcgcggagc
     2041 tgctcattca cggccacgag ccgtgcgagt cgggcttcag cgcccccaac gcccggcagg
     2101 tcatcctcga cggctgctgc tcgcatgcga cctacttgat tctgcccgtc ggccccaagc
     2161 tgtcgcaggc cgacgtcgtc gcccgcatca ccagcctgca caaccgccgc gaaacgtgcc
     2221 cggtgtgagc gcctcccgct tcttgtaggt cccgcctgcc gggcgggacc ttggtggcgc
     2281 ggaaaacgaa gagggtgcaa ctcgctgcgc ttcaaggtcc cttccggcag aaggggccta
     2341 ctgacgggtg agggcgagcg gaggacgatt aggattaaga ttaggattaa gattaggacg
     2401 cagtcatgat cttactcgtc atcgttctct cctcggcgaa gccgtgaacc cgcccgtcaa
     2461 gcagcccaag cgcccggcct ttcagttcag cctgctgggg ctgatggtcg tgatgttcgt
     2521 cgtcgctgcg gcagccgcgc cgggatacta catgctccgc ggccagacgc tgccccagtc
     2581 gcgcctggtg ggcatgctga tggtcctggc cggcccgctc ctcttgatga cgctgatcag
     2641 cgtcgtcctc tcgctcaccc accgcggcca gcgctgactt tggtggcttc ttgagcggcg
     2701 atccgcgaca atgaggcgta gcataagcgt cctcgcttgt gcagcggtcg tgcgcggatg
     2761 tcgatgagca agcgaggacg cttgctctac gcttgccatg cgccctctga ttctcctcgc
     2821 cgcagtactt gtattcgcag cggcaaactg cgcgcaagcg cagcctgcga ggagcgccgt
     2881 gcagccgccg gcgacgcacg ctcgacttcg ggcggcaggc gtcgagccga cgctggaagg
     2941 agtccgcgac tacctggaat cgctgctgga cccccagctc gaaaagcagg tcgatcgctg
     3001 gatcgccgag ctgggcgatc cgcagttcga agtccgcgag cgggcctcgc tgcagctggc
     3061 cagtctcgac ttcgtgccca cgctgaagtt gcaggcggcg gcgaccagcc ccgatcccga
     3121 gcgggcctgg cgggccaagg tggccctgaa gggcgcttcg cccgaggccg gactcttgcc
     3181 ggcggcgctg cggctgatcg aggaggagaa gctgcccgtc ggcctgccgc tgctggtgca
     3241 gcttcgcagc cggatcgttt cgccggcgat gcaggaagcg gcggcccggg cgatgctggc
     3301 gatcgtggcc gaggccgacc gagcggcgct ggccgagctg caaaggcacg aggacgctgc
     3361 tatccgcggc gtggccggcc ggctgctggc gcggctcgat cgcccgccgg ccaaatacca
     3421 ggagggctcg ttcgacgcgg tcaagctgac gcccggcggc atcagcggcg gcggccggaa
     3481 tcttatcgag ggctgggaat tccggccgaa ggccgacatc gtcgtgacgc acctgggcct
     3541 cttcgacagc ggcggcgacg gcctggccgc ccagcacgaa atcgccatct gggacatcga
     3601 ggacccgtcg gggccgctga cgcaagagac gatggttccc ggcacgactg cgccgctggc
     3661 cggcgcgttc cggctgataa cgacgcaccc gatcaagctc tcggccggcc ggcggtacgc
     3721 gatcgtcgcg cactatcccg acgcccgcga ctcgaacgtc ggcatgatca acccggcagg
     3781 cctgaccatc gattacgcgg agcacgtcga agccctgggc cgccgctatt cgttcccgca
     3841 cccgtcgatg gcgtttcccg ccgagttcgc tgaaggcccc aagcattcca agcacggccc
     3901 gactttccgc tattcgctcg ccgccgatcc gaaggacgaa aggaaaccgt agccacatgt
     3961 ccgctgtccc accccgatcc atgatcgccc tcgccctgac tgccgcgctg gccgccttgc
     4021 ccgctttcgc cgccgacttg ccggcggaag agcgcgacgc ggggttcgtg gagctgttca
     4081 acggcaagga cttcaccggc tggcggttcg gcgatgggcc gtcgccgccg gcggagattc
     4141 cggcgaattg gagagtcgag gagggcgtca tcaaggtcac cggcggcggg gctccgcatt
     4201 tgggatcgga aaaggcctac ggcaatttcg agctgcgcct cgagtggcgc ggcctgaaaa
     4261 acggatacaa cagcgggctc tatgttcgca gcggcaaaaa ggtcggcagc aaccagatca
     4321 acctggccca caaaagcgag ggcgcattca tcggcggcaa ggtcaccggc gcgaaggccg
     4381 tcggcgactt gcaaaagccc gccggcgagt ggaacgagtg gcgcgtcacc gcggtcggcg
     4441 acaagctcac gctccactgc aacggaaagc tcgcctggga agccaccggc ttcgcgcccg
     4501 ccgaaggcca catcggcatg caggccgaag gcgccgccat ggagttccgc aacatccgat
     4561 tgcgggagat caagtagcag gaagaatcgc cttgcaattc tcggttgcca aactgctcgg
     4621 atcgactgct ttcgtcgcga tcggcctggc agcgctggcc agtgcttcgc acctgctggc
     4681 cagcgccttc ctgtcggtga cgatcctggc gctctgtgct gcggtcatgg gcgctatcgt
     4741 gacgagcggt gcagcgcggg ccttctggct ggctttcttg ttgttcgcag gcgttcatct
     4801 gctcctcacg ctgggccctt ggctggacga tgtgaccggc gagattctcc tgtcgcgcct
     4861 ggcaatcgac gccttgggaa gggcgatggg ccaggacgta gccgatcatc aaaggatgcc
     4921 cggcgtgtgg ctcaatttgc cgtatgcgac cgcgggatcg agttatggct tcctgaattt
     4981 cgtggtgatc ggacagtgct ggttctcgct cgcgctgggt tgtgtcgccg gcgaattggc
     5041 cggctttttc gctggtcggg caagcgggga aaggcgacgc taggcggagc tatttccacc
     5101 gaaaaatccg cagcgcgatc gcgaatgtcg ttgcgccgta cgcggccaag atcgccgcct
     5161 gctgccagag cgcggccagc gatttgcctt ccaggatgat cccacggagc agctggttca
     5221 gcgcggtcag cggcagcagg ttgatcagcg gctgcacgac ctcagggaaa cgctcggacg
     5281 aaaagaacac acccgagaga atccacatcg gcagcatgat caggttcatc aggccgctga
     5341 ccgtttccag cgtctccgcc cggctggcca cgagcaggcc aatcccggaa aaggcgacgc
     5401 cgccgacgag caccgcggcg gtcaggtcga ggtagcttcc ctggcagcgg acgccaaata
     5461 cgaagtagcc gaacacgagc aggatcacga tgtcggccag cgagaacatc agccggctga
     5521 tcatcacggc gagcagaaag tcgctgcggc gcatcggcgt cgccagaaac cgcttgagca
     5581 gcttgcggac ccgcatatcg acgatgacga agcccacgcc ccacatgccg ccccccatca
     5641 gccccatccc gatcatcccc ggcagcaaaa agtcgatgta ccgcgagccg gccttttgga
     5701 ggtgctgcac cggggccggc ggcggcgact cgtccgtccg caggagcagc gcttcgactg
     5761 cgtagcgggc cacgcggctc tcggcccggt gcggctcgtc ccacaggcga tacgccggct
     5821 ggctggccgt cggcagaact tcgatgacca ggtcggtctt gccggcttgc aggcgctttt
     5881 gccaatcggc tggctggatg ttgagcttga atcgcgaatc ggcggcgaga cggctttgaa
     5941 tctcgctgag ggctgccggc ggggtcgcag agcgcgacca ctggtcgcgg ctttggtcca
     6001 cgatgagatc gaccgcgatg atctcggtgg gattgtcgcg gaaggcgatc cccagcgaca
     6061 tcatcatcag aatcgggaag ccatagaccc agaaaatggc cgccggctgg cgggtgaatt
     6121 ccttcacccg cgccagcgtc agttgccaga tcggctcgag cctacggctg gccattgggc
     6181 tgttcctcca gatggcggcc ggtgagcgag acaaagacgt cttccagcgt ggcgtgccgc
     6241 gtcgtcaggt gggccagcgc caagccgcgg ctctgcagca acgccaaaag cgccggcagc
     6301 gccacatgcg gctgggtgac ggcgaggctg tacgattcgt cctggctgcg ggcc
//
LOCUS       DASRJB010000531         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1836741, whole genome shotgun sequence.
ACCESSION   DASRJB010000531 DASRJB010000000
VERSION     DASRJB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836741"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..2988
                     /locus_tag="VFB80_13805"
     CDS             34..2988
                     /locus_tag="VFB80_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN34897.1"
                     /translation="MSISSLIVLWLIACATLPQAAAAPPKSLKLGIVQVGLEPTLEAS
                     RDKIAAKIREAKAEGCRVVVFPETSLFAPPAATTPQIDAAVEELRKVAAASDVYVLLG
                     GLYKRNAAEKPFERLLVIDPRGEIIQTYNKMWSDARFPDCPGLFEIDGVPCAAAICAD
                     RWIRSVEELPAMAGAKILFEVSNNYADEWLPELGWFWYVPRAIRNEVFVVFANSAKED
                     RAQNTPGHGHSAVINPDGSLLASLGEESDKLLVAELDLTRATAGEAQRRKSHPQFKEF
                     WTAGVSILSGQSVAAPPFRALTSPQVELKIAAAQMACSTSLDENAAHINELIYQAKDG
                     GADLVVFPELALTGAEGADIRSAKQADLEAYLASVQVAARNTGLYVVCGLPWRERDRL
                     LNTAVAIGPDGQLLTRYAQLVVERPELFAAGTSTADMWFQVKGVPCVLTIGRDALWSE
                     MAEMAALRGAQVHLHLAYDRQTSPAADLRRQQLWVNLASFRTFTATVNAASPRTIPNP
                     SSPASGGSAIWDDFHRGQNRKLGGYGPYSAVRVAHAKQDETMLFATQKIPATNPQLSI
                     FTGKTNRQMTPWYATGAALIKPTGKETTEPIAGPFIDGKFRGRIAYSADGNHNDPDDW
                     AASPVALAIFAEAGLKDRLVHFDYNCILPQTDPAWEKTHAESVLGAAERYGYRPELFH
                     DCRQDLSSAVASIARAIDASTAEDPLYFIVAGPMEVPYLGIQKAQPEKRQHVYCISHS
                     RWNDGFARQYKFTHSKRSVIETGVHWVQIRDQNRLLSKSPYGQPAPADSWQAYHWMRD
                     SADAKVRWLWERLKVSTRPDPSDAGMAYFLATGDEDADPAKLQKLLDNHAAPQPLASR
                     EQIRLEAENFRELKGCGVEDTNDRAASHRLQVKLTGDSGQLATRFAQPYAADGKYDVE
                     IRCAPAPIARGRVQLFVDGVAQGSPWQGGEGDAGWITHTVRGVPVRLGSEIRLDAVGE
                     GVRIDYVQFNRQ"
     gene            3019..>3478
                     /locus_tag="VFB80_13810"
     CDS             3019..>3478
                     /locus_tag="VFB80_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN34898.1"
                     /translation="MSSRSLAAATTLPATLPVPAAEAPAMPSTQQLRIAVLPGDGIGI
                     EVMAEAVRVLRAVEAAGSGLRLELSELPAGAGEYQRRGDPLPAETLAACRASDAVLLG
                     AMGWPEIRWPDGREIAPQLDLREQLDLYCGLRPIRLYHAADTPLKGCQAGD"
BASE COUNT          592 a         1197 c         1157 g          532 t
ORIGIN      
        1 cgctgctcga gggtgccggg gatgaaacac agcttgagta tttcgtccct gattgttctg
       61 tggctgattg cctgcgccac gttgccacag gcagctgccg ccccgccgaa gtcgctcaag
      121 ctcggcattg tgcaggtcgg cctggagccg accctggagg ccagccgcga caagatcgcg
      181 gccaagattc gcgaggccaa agcggagggc tgccgcgtcg tagtcttccc cgagacttcg
      241 ctcttcgccc cgcccgcagc taccacgccg caaatcgacg ccgccgtaga agaattgcga
      301 aaggtcgcgg ccgccagcga cgtgtacgtg ctgctcggcg gcctttacaa gcgcaacgct
      361 gccgaaaagc ccttcgagcg gctgctggtg atcgatccgc gcggcgaaat catccagacc
      421 tacaacaaga tgtggtccga cgcgcggttt cccgattgcc cgggcctgtt cgagatcgat
      481 ggcgtaccct gcgcggcggc gatctgcgcc gaccgctgga tccgcagcgt ggaagagctg
      541 ccggcgatgg ccggggccaa aatcctgttc gaggtctcga acaactacgc cgacgagtgg
      601 ctgccggagt tgggctggtt ctggtacgtg ccgcgggcga tccgcaacga ggtgttcgtc
      661 gtcttcgcca attcggcgaa ggaggaccgg gcccagaata cgcccggcca cgggcacagc
      721 gcggtgatca acccggacgg cagcttgttg gcgtcgctgg gcgaggaatc ggacaagctg
      781 ctcgtcgcgg agctggacct gacgcgagcg accgccggcg aggcccagcg gcgaaagagc
      841 catccgcagt tcaaagagtt ctggacagcg ggcgtgtcga tcctgagcgg ccaaagcgtc
      901 gctgctccgc cgttccgcgc gcttacctcg ccgcaggtcg aactgaagat cgccgcggcc
      961 cagatggcct gctcgacctc gctggacgaa aacgccgcgc acatcaacga attgatctat
     1021 caggcgaagg acggcggcgc ggacctcgtc gtctttcccg agctggcgct gaccggcgcg
     1081 gagggcgcgg acatccgcag cgcgaagcag gccgatctgg aggcctacct ggcgtcggtt
     1141 caagtcgcgg cccgcaatac cgggctgtac gtcgtctgcg gcctgccctg gcgcgagcga
     1201 gatcggctgc tgaatacggc ggtcgcgatc ggcccggacg gccagctgct gacgcggtac
     1261 gcccagctcg tcgtcgagcg gcccgagctc tttgcagcgg gaaccagcac tgccgacatg
     1321 tggttccagg tcaagggagt gccctgcgtc ctcacgatcg ggcgcgacgc gctgtggagc
     1381 gaaatggccg agatggcggc cctccgcggc gcgcaggttc atttgcacct ggcctatgat
     1441 cgccagacgt cgcccgcggc ggacctccgc cgccagcagt tgtgggtgaa cctggcttcg
     1501 ttccgcacct tcacggcgac ggtcaacgcc gcctcgccgc ggacaattcc gaatcccagc
     1561 agcccggcca gcggcggcag cgcgatctgg gacgacttcc accgcggcca gaaccgcaaa
     1621 ctcggcgggt atggtcccta ctccgccgtc cgcgtggccc acgcgaagca ggacgagacg
     1681 atgctcttcg ccacccagaa gatccccgcg accaacccgc agttgagcat cttcaccggc
     1741 aagaccaatc gccaaatgac gccctggtat gcgaccggcg cggccctcat caagccgacg
     1801 ggcaaggaaa ccaccgagcc catcgcgggc ccgttcatcg atggcaagtt ccgcggccgc
     1861 atcgcctaca gcgccgacgg caaccacaac gatcccgacg actgggccgc gtcgcccgtt
     1921 gctctggcga tcttcgctga ggcgggcctg aaggaccgcc tcgtccactt cgattacaac
     1981 tgcattctcc cgcagaccga tccggcctgg gaaaagacgc acgccgaaag cgttctcggc
     2041 gcggccgagc gctacggcta ccggccggag ctgtttcacg actgccgcca ggacctgagt
     2101 agcgccgtgg ctagcatcgc ccgggcgatc gatgcctcca cggccgagga cccgctctat
     2161 ttcatcgtcg ccgggccgat ggaggtcccc tacctcggca tccagaaggc ccagcccgag
     2221 aagcggcagc acgtctattg catctcgcac agccgctgga acgacggctt cgcccggcaa
     2281 tacaagttca cgcacagcaa gcggagcgtg atcgagacgg gcgtgcattg ggtgcagatt
     2341 cgcgaccaga accgcctcct gagcaagagc ccctatgggc agcccgcgcc ggccgactcg
     2401 tggcaggcgt atcactggat gcgggattcg gccgacgcga aggtccgctg gctgtgggag
     2461 cggctcaagg tttcgacgcg gcccgatccg tccgacgcgg gcatggcgta tttcctggcc
     2521 accggcgacg aagacgccga cccggccaag ctgcaaaagc tgctggataa ccacgccgcg
     2581 ccgcagccgc tcgccagccg cgagcagatc cgcctggaag ccgaaaactt ccgcgagctg
     2641 aagggatgcg gcgtcgagga cacgaacgat cgcgcggcgt cccaccggct gcaagtcaag
     2701 ctgacgggcg actcgggcca gctggcgacg cgttttgccc agccctacgc ggctgacggc
     2761 aagtacgatg tggaaatccg ctgcgctccg gcgccgatcg cccgcggccg cgtgcaattg
     2821 ttcgtggacg gcgttgccca gggcagtccc tggcaaggcg gcgagggaga cgcgggctgg
     2881 atcacgcaca ccgtgcgcgg cgtgcccgtt cgcctgggca gcgagattcg cctggatgcc
     2941 gtcggcgaag gagtgcggat tgactacgtg cagttcaatc ggcagtgagt tttcgtccgc
     3001 cttcaccttg gggatcttat gtcttcccgc tctctggccg ccgcaacgac cttgcccgcc
     3061 acgctgccgg tgcccgcggc ggaagcgccc gcgatgccct ccacgcagca gcttcgcatt
     3121 gccgttctgc cgggcgacgg catcggcatc gaagtgatgg ccgaggcggt ccgcgtcttg
     3181 cgggccgtgg aagccgcagg cagcggcctg cggctggagc tgagcgagct gccggctggc
     3241 gcgggcgagt accagcgccg cggcgatccg ctgccggcgg aaacgctcgc cgcctgccgc
     3301 gcgtccgacg cggtgctgct gggggcaatg ggctggcccg aaatccgctg gcccgacggc
     3361 cgcgaaatcg ccccgcagct cgacctccgc gagcagctcg atttgtattg cggcctgcgg
     3421 cccatccgcc tctatcacgc ggccgacacg cccctcaagg gctgccaggc cggcgaca
//
LOCUS       DASRJB010000532         7864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3248220, whole genome shotgun sequence.
ACCESSION   DASRJB010000532 DASRJB010000000
VERSION     DASRJB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248220"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1180)
                     /locus_tag="VFB80_13815"
     CDS             complement(41..1180)
                     /locus_tag="VFB80_13815"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34899.1"
                     /translation="MPKFLCRRPQSRGFTLVELLVVIAIIGVLVALLLPAVQAAREAS
                     RRSQCLNNLKQIGLGVHNFESSHSKLPNSGQCDSTGSNSTTYMIHSTATQILPYIEQQ
                     AVYDMFDHEASPATFGATLSGTQWTSNGAILHLNAKGRPYDDPANARWQQAAKTQIST
                     FVCPSAPLKKSTRDPVHGYGGWDYMFIALSDVDETQGSATFKQRTVPAGSAAWLAQVV
                     PGMLTCDGARFANTTDGTSNTFLCIEDAGRAHPNVVKFGAASSRNSPMASPADAMPSR
                     LRRVFAWADPDAVTNGFSGPNNATVAGDRVAKVNNHKAPVGGPTTCPWSTNNCGPNDE
                     PFSFHPNGVNAVMGDGSVRFVLDSMDALLLKALCGANDGKVTQLD"
     gene            1410..1982
                     /locus_tag="VFB80_13820"
     CDS             1410..1982
                     /locus_tag="VFB80_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34900.1"
                     /translation="MVIIWGTTHAGKVDEVPGGMFHVVTQFGHVYYIPLIPTASYIVL
                     EKTADGGFRGTDIPLSFKSILAGWLRGGSVIAIIGACIGLAICLLSQKAGPFDWVLPV
                     VIGCAAGGVLFLTYKLAFFTQASYERAMELAQRVGLNDTGLLMLEVAYGRMNAAQAEA
                     ELARREAQAAAQQPVQAELADPQPGGFLVN"
     gene            2007..4016
                     /locus_tag="VFB80_13825"
     CDS             2007..4016
                     /locus_tag="VFB80_13825"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HZN34901.1"
                     /translation="MAADNSVSRRGFLENALLGAGALAALPVVFQPAAAAQRAAAAVP
                     HPLDPLTADEIEAATAILDEAGVLGRGFRFISTSLLEPAKTEMQAWKPGQPLARLAQV
                     VLLDRESGLGFEAIVDLGKHAVSGFKKLPAGVQPAMFIGECQECEQAVRQSPKFLEAL
                     AKRGIDDANLVIIDCWSAGNYGNEPAGYKNRRLTRPLFFLKPDEAANPYSRPIDGLLA
                     VIDLNTSEVLEVRDFGPVPLPPEEGRWDRDSIGKFRDGLMPLEIVQPEGPSFTVSGRD
                     VRWQHWRFRIGFNAREGLTLHDVRHWDAGRERPILHRASICEMLVTYGDPGEPYYRKN
                     PFDIGEYLLGAMANPLSQGCDCLGTIRYFDAHIVTERGDALAIKNAICLHEEDAGLLW
                     KHTEQRMDQAEARRSRRLVVSYIATAGNYDYAFYWHFYQDGSLACEVKLTGIMNTTAI
                     HRGQTPEFGVEVAPQLAAPFHQHVFCARLDLDIDGNENSVQEVNTVGLPRAPKNPHGH
                     AFRAEVTPLAIESAARRHVNGAAGRFWRVVNPQRKNRLGQPVGYRLIPGENCPLLLQP
                     DSAVARRAGFTQHQLWVTPHRDDERFAAGEYPNQHAGGDGLPRWTTADRSVAATDIVL
                     WYVFGHNHVPRLEDWPVMPVSSLGFALKPDGFFERNPALDLPPPG"
     gene            4057..5292
                     /locus_tag="VFB80_13830"
     CDS             4057..5292
                     /locus_tag="VFB80_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN34902.1"
                     /translation="MPFRQHILDNGLEIVAEVSPDAYSSAFAFFVRTGARDENDAISG
                     VSHFLEHMVFKGSANRTAAEVNRDLDDLSAGSNAYTSEEQTVYYATTLPEDQEQILEL
                     LADMMRPVLRQDDFDTEKQVILEEIAKYDDQPPYGAHEKCMAAFFGEHPLGNSVLGTT
                     ASVGGLARDAMLHYFERRYSPRNMVLAAAGNVAFDALILQAQNYCGRWQMFDAPRELP
                     PARPRSGFQVIHKPLAVQQYVVQLAAAPSATDDDRYAARLLATIIGDETGSRLFWELI
                     DTGLAEYAAISTQEYQGAGVFYIGMSCLPEEAQSNLRRLSDLFAKLEADGITAEELSQ
                     AQNKVCASIVLQAERPTNRMFSIGSSWLQRRQYKTVREAVEAYRRVTLDDLHRVLKKY
                     PLSQTATVAIGPLTELHAP"
     gene            complement(5350..6117)
                     /locus_tag="VFB80_13835"
     CDS             complement(5350..6117)
                     /locus_tag="VFB80_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN34903.1"
                     /translation="MRPNAVKAKLAAGGTALGAFMFEFATTGVARLAAEAGADFAVFD
                     MEHSGWSIETIRMLLATCGGTSLVPLVRVPTTEYDLIARALDMGAAGIMVPMVESAEQ
                     ARRIVEFAKYPPIGRRGAAFGIAHDNYTGGDTLAKIAAANRETLLIAQVETAAGVRHV
                     EEIAAVDGIDVLWIGHFDLSNSLGIPVQFTHPMFQDAVAKVLAACATHRKTAGFMCAD
                     VADGKALLAKGFRMLAYSGDLWLYQQALRAGLAELRR"
     gene            complement(6131..7159)
                     /locus_tag="VFB80_13840"
     CDS             complement(6131..7159)
                     /locus_tag="VFB80_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34904.1"
                     /translation="MSKFRVGITRDFLRADGTLGLGDIGLALLDLPHVEREFLISSDG
                     EIPPDVADEYDALLVLAPRVTERTVTGCKRLGLVARFGVGYDTVDVPACTQHDVLLTI
                     TPDGVRRPVAVSALTLILALAHKLLIKDKLTRAGRWSEKLDHLGMGLAGRTLGVIGLG
                     NIGREVFRVALPLAMRHVGHDPFVGADQAAAMGVQWLVLDQLLAEADFVVICCALTAE
                     TRRLIDARRLALLKPTAYLINVARGPIVDQAALTAALQQRRIAGAGLDVFDPEPIDPR
                     DPLLALDNVILAPHALCWTDECFLGNGRSACEAILDYAAGRVPRHVVNRDVLDRPGMQ
                     AKLKRLAT"
     gene            7225..>7864
                     /locus_tag="VFB80_13845"
     CDS             7225..>7864
                     /locus_tag="VFB80_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN34905.1"
                     /translation="MTLARVIIADFQHGSLEVEQEILKGLAAVAALDVTSEEQMWGRV
                     EDADAIMVYHTLKVTRATIERLTRCRLIVRCGVGYDNVDHVFAKSRGIPVANVPDYGN
                     EEVADSAIGMMLALTRGINLQNTVLRPAGSKWRYDNVAPLYRLRGRTFAVLGLGRIGT
                     AAALRAKALGMNVIFYDPYKADGYDKSLSVRRVETLAELLGEAFVLSCHSPLT"
BASE COUNT         1357 a         2612 c         2584 g         1311 t
ORIGIN      
        1 ctcgtgaggc cgttgccgtt tttcggccgg tgtgcagcgg ttagtcgagt tgcgtcacct
       61 tgccgtcgtt ggcgccgcag agcgccttga gcagcagggc atccatgctg tccagaacga
      121 aacggaccga gccgtcaccc atgaccgcgt tcacgccgtt gggatggaag ctgaagggct
      181 cgtcgttcgg gccgcaattg ttcgtgctcc acgggcaggt ggtcggtccg ccaaccgggg
      241 ccttgtggtt gttgaccttg gcgacccggt cgcccgcaac ggtggcgtta ttcggaccgg
      301 agaagccgtt ggtgacggcg tccgggtcgg cccaggcgaa cactcgccgc agtcggctgg
      361 gcattgcgtc ggcagggctg gccatggggc tgttccggga tgaggcggcg ccgaacttga
      421 ctacgttggg atgggcccgg ccagcgtcct caatgcaaag aaacgtgttg cttgtgccgt
      481 cagtggtgtt ggcaaaccgt gcgccatcgc aagtcaacat gccaggcaca acctgagcca
      541 gccaggccgc ggagccggcc ggcaccgtcc gctgcttgaa ggtggccgag ccctgcgtct
      601 catccacgtc gcttagagca atgaacatgt agtcccaccc gccatagccg tgcactgggt
      661 cgcgagtcga ctttttcaag ggcgcgcttg ggcagacaaa ggtcgaaatc tgggtcttcg
      721 ccgcttgctg ccagcgggca ttggcggggt catcataggg gcggcccttg gcattgaggt
      781 gcaagatcgc gccgttcgaa gtccattgcg tgccggaaag cgtcgctcca aacgtcgcag
      841 gggatgcctc gtgatcgaac atgtcgtaca ccgcttgctg ctcgatgtac ggcaggattt
      901 gcgtggccgt cgagtgaatc atgtaggtcg tcgagttgct gcccgtggaa tcgcattgcc
      961 ccgagttcgg caacttggaa tgactcgatt cgaaattgtg aactcccaag ccgatttgct
     1021 tgagattatt caggcactga gagcgccgcg aagcctcgcg cgccgcttga acggcgggca
     1081 atagcaaggc gaccaggacg ccgatgatgg cgatgacaac gaggagttcg acgagcgtaa
     1141 agcctcgcga ttgaggcctg cggcagagaa acttcggcat ggatttcccc agagaaaatg
     1201 aggcggcgtc ggttgatttg actccagttg aaaatcgaga attaatatga aggttcaatg
     1261 aaggcgcgat gaaaaccccg ctagcaatgg gcgagctttt aaccaattgc cttcccccga
     1321 agagagagga agccctgccc cctttttctt gagtctcagg ggcggttcgc tatgctagag
     1381 ccgttacttc acacagctgg gagccagcca tggtcatcat ctggggcacg acgcacgccg
     1441 ggaaagtcga tgaagttccc ggcggcatgt tccacgtcgt cacgcagttc gggcacgtct
     1501 actacatccc gctcattccc accgcttcgt acatcgtgct ggaaaagacg gctgacggcg
     1561 gcttccgtgg cacggatatc ccgctgagct tcaagtcgat cctcgccggc tggctccgcg
     1621 gcggcagcgt catcgccatc atcggggcct gcatcggcct ggcgatctgc ctgctcagcc
     1681 agaaagccgg gcccttcgac tgggtgctgc ccgtcgtcat cggctgcgcg gccggcggcg
     1741 tgctctttct cacgtacaag ctcgcgttct tcactcaagc gtcctacgag cgggcgatgg
     1801 agctggccca gcgcgtcggc ctgaacgaca cgggcctctt gatgctcgag gtagcctacg
     1861 gccggatgaa cgccgcccag gccgaagccg agctggcccg ccgcgaagcc caggcggcag
     1921 ctcagcagcc ggtgcaggcc gagctggcag atccgcagcc gggcgggttc ctggttaact
     1981 agattcagca accacggaac cgattcatgg ccgctgacaa ttcggtctcc cgccgcggat
     2041 tcctggagaa cgcgctcctg ggcgccggcg ccctcgcggc gctgcccgtg gtctttcagc
     2101 ccgccgcagc tgctcaaagg gcagccgctg ccgtcccgca tccgctcgat ccgctgacgg
     2161 ctgatgagat cgaagcagcc accgccatcc tggacgaggc cggcgtgctc ggccgcggct
     2221 tccgcttcat cagcacctcg ctgctggagc cggcgaagac cgagatgcag gcctggaagc
     2281 ccgggcagcc gctggcccgg ctagcgcagg tcgtgctgct cgatcgcgag agcggcctcg
     2341 gcttcgaggc gatcgttgat cttggcaagc acgctgtgag cgggttcaaa aagctgccgg
     2401 cgggcgtgca gccggcgatg ttcatcgggg agtgccagga gtgcgagcag gcggtgcggc
     2461 aatcgcccaa attcctggaa gcgctggcca agcgcggcat cgacgatgcg aacctggtca
     2521 tcatcgattg ctggtcggcg ggaaactatg gcaacgaacc ggccggctac aagaaccgcc
     2581 ggctgacgcg gccgctgttc ttcctgaagc ccgacgaggc ggcgaatccc tattcccgcc
     2641 cgatcgacgg cttgttggcg gtcatcgatc tgaacaccag cgaagtgctg gaggtccggg
     2701 actttggccc cgtgccgctg cccccggagg agggccgctg ggatcgcgat tcgatcggca
     2761 agtttcgcga cgggcttatg ccactggaga tcgtgcagcc ggaagggccg agcttcacgg
     2821 tcagcggccg cgacgtccgc tggcagcatt ggcggttccg catcggcttc aacgcccgcg
     2881 aggggctgac gctgcacgac gtgcggcatt gggacgccgg ccgcgagcgg ccgatcctgc
     2941 accgggcgtc gatttgcgaa atgctcgtga cctacggcga tccgggcgag ccgtactatc
     3001 gcaagaaccc gttcgacatc ggcgagtacc tgctgggggc gatggccaat ccgctgtcgc
     3061 agggctgcga ctgcctgggc acgatccgct atttcgacgc ccacatcgtc accgagcgcg
     3121 gcgacgcgct ggcgatcaag aacgcgatct gcctccatga ggaggacgcc ggcttgcttt
     3181 ggaagcacac cgagcagcgc atggaccagg ccgaggcccg ccgctcgcgg cgcctggtgg
     3241 tttcgtacat tgcgacggcg ggcaactacg actacgcgtt ctactggcac ttttaccagg
     3301 acggcagcct ggcctgcgag gtgaagctga ccggcatcat gaacacgacg gcgattcatc
     3361 gggggcagac gccggagttt ggcgtggagg tcgccccgca gctcgccgcc cctttccacc
     3421 agcacgtgtt ctgcgcgcgg ctcgacctgg acatcgacgg caacgagaac tcggtgcaag
     3481 aggtcaacac cgtcgggctg ccgcgcgctc cgaaaaaccc gcacggccac gcgttccggg
     3541 cggaagtcac gccgctggca atcgagtctg ccgcgcggcg gcatgtgaac ggggcagccg
     3601 ggcgattttg gcgggtggtc aacccgcagc ggaagaaccg cctgggtcag cccgtcggct
     3661 atcgcctgat tccaggcgag aactgcccgc tgctattgca gcccgattcg gccgtggccc
     3721 ggcgagcagg cttcacgcag caccagctgt gggtcactcc gcatcgggac gacgagcggt
     3781 tcgcggctgg ggaatatccg aaccagcacg ccggcggcga cggcctgccc cgctggacca
     3841 ctgccgaccg cagcgtcgcc gccaccgaca tcgtcctgtg gtacgtgttt ggccacaacc
     3901 acgtcccgcg gctcgaggac tggccggtca tgccggtttc ttcgctaggt tttgcgctca
     3961 agcccgacgg cttctttgag cggaatcctg ccctcgactt gccgccgccg ggatgaacaa
     4021 tggaagtgat ccccagctcc acggaaaagg acccgcatgc cctttcgcca gcacatcctc
     4081 gataacggcc tggaaatcgt ggccgaggtc agccccgacg cctattcttc ggcgttcgcg
     4141 ttcttcgtcc gcacgggggc gcgggacgag aacgacgcga tctcgggcgt gagccacttc
     4201 ctggagcaca tggtcttcaa gggctcggcc aaccgcacgg cggccgaggt caatcgcgac
     4261 ctcgacgacc tgagcgccgg cagcaacgcc tataccagcg aggaacagac ggtctattac
     4321 gcgacgacgc tgcccgaaga ccaggagcag atcctcgagc tgctggccga catgatgcgg
     4381 ccggtgctgc ggcaggacga cttcgatacc gagaagcagg tgatcctgga ggagatcgcc
     4441 aagtacgacg atcagcctcc gtacggggcc cacgagaagt gcatggcggc gttttttggc
     4501 gaacatccgc tgggcaacag cgtgctaggc acaaccgcca gcgtcggcgg cctggcccgc
     4561 gacgcgatgt tgcactattt cgagcgccgc tacagcccgc ggaacatggt gctcgccgcg
     4621 gcgggcaacg ttgccttcga cgcgcttatc ctccaggccc agaactactg cggccgctgg
     4681 cagatgttcg acgccccgcg cgagctgccg ccggcgcggc cgcggtccgg ctttcaagtc
     4741 atccacaagc cgctggccgt gcagcaatat gtcgtgcagc ttgccgcggc gccgtcggcg
     4801 accgacgacg accgatacgc cgcgcggctg ctggcgacga tcatcggcga cgagaccggc
     4861 agccggctgt tctgggagct gatcgacacg ggcctggccg agtacgccgc gatcagcacg
     4921 caggagtacc agggggccgg cgtgttctac atcgggatga gctgcctgcc cgaggaggcc
     4981 cagagcaatc tccgccggct gtccgatctc ttcgccaagc tcgaggctga cggcatcacc
     5041 gcggaggagc tgtcgcaggc tcagaacaag gtctgcgcga gcatcgtgct tcaggccgag
     5101 cggccgacga accggatgtt ctcgatcggc agcagttggc tgcagcggcg gcagtacaag
     5161 accgtccgcg aagcggtcga ggcctatcgc agagtaacgc tcgacgactt gcaccgtgtg
     5221 ctgaagaagt acccgcttag ccagaccgcc accgtcgcca tcggcccgct gacagagctg
     5281 cacgcgccgt aggcgacgct gccagcgtcg ccctcgttgc tgtgccgacg ctggcagcgt
     5341 cggctacgat caccgcctca gctcggccag ccccgcccgc agggcctgct gatacagcca
     5401 caaatcgccg ctgtaggcga gcatgcggaa tcccttggcc aacagcgcct tgccgtcggc
     5461 gacgtcggcg cacatgaagc cggcggtctt gcggtgggtc gcgcaggcgg ccagcacctt
     5521 ggccactgcg tcctggaaca tggggtgcgt gaactgcacg ggaattccca gcgagttcga
     5581 gaggtcgaag tgcccgatcc agagtacgtc gattccgtcc accgcggcga tttcctcgac
     5641 gtgccgcacg cccgcggctg tttcaacctg cgcgatgagc agcgtctcgc gattagcggc
     5701 ggcgattttg gccagcgtgt cgccgcccgt gtagttgtcg tgggcgatgc cgaacgccgc
     5761 cccgcgccgg ccgatcggcg gatacttggc gaactcgacg atccgccggg cctgctcggc
     5821 cgattcgacc atcggcacca tgatgcccgc cgcgcccatg tcgagggccc gggcaatcag
     5881 gtcgtactcg gtcgtcggca cgcggaccag cggcacgagg ctcgtcccgc cgcaggtcgc
     5941 cagcagcatg cggatcgtct cgatcgacca gccggagtgc tccatgtcga agacggcgaa
     6001 gtccgcgccg gcctcggcgg ccagccgggc gacgcccgtc gtcgcgaact cgaacatgaa
     6061 cgcgcccagc gccgtgccgc cggcggcgag tttggctttc actgcattcg gtcgcatgct
     6121 ctttgccttg tcaggtggcc agccgcttca acttcgcctg catcccgggg cgatcgagca
     6181 cgtcgcggtt gacgacgtgc cggggaacac gccccgcagc ataatcgaga atcgcctcgc
     6241 acgccgagcg gccattgccc agaaagcact cgtcggtcca gcagagcgca tgcggcgcga
     6301 gaatcacgtt gtcgagcgcc agcagcgggt cgcgcggatc gatcggctcc ggatcgaata
     6361 cgtccagccc cgcgcccgcg atccgccgct gctggagggc cgcggtcagc gccgcctgat
     6421 cgacaatcgg tccgcgggcg acgttgatca ggtaggccgt cggcttgagc agcgccagcc
     6481 gccgcgcgtc gatcagccgc cgcgtttcgg ccgtcaacgc gcagcagatg acgacgaaat
     6541 cggcctcggc cagcagctgg tccagaacca gccactgcac gcccatcgcg gccgcctggt
     6601 cggccccgac aaaggggtcg tgtcccacgt gtcgcatcgc caggggcagc gccacgcgga
     6661 acacctcgcg gccgatattg cccaggccga tcacgcccag agtccgcccg gccagaccca
     6721 tgcccaagtg gtcgagcttc tcgctccagc gcccggcgcg tgtgagcttg tccttgatca
     6781 gcagcttgtg ggccagggcg agaatcagcg tcagcgccga cacggcgacc ggccgccgca
     6841 cgccgtcggg cgtgatcgtg agcagcacgt cgtgctgcgt gcaggccggc acatccaccg
     6901 tgtcgtagcc cacgccgaag cgggccacca ggcccagtct cttgcagccc gtcaccgtcc
     6961 gctcggtcac gcgcggcgcg agaaccaaga gcgcgtcgta ctcgtcggcg acgtcgggcg
     7021 gaatctcgcc atcgctggag atcaggaact ctcgctccac gtgcggcaga tcgagcagcg
     7081 ccaggccgat gtcgccaagc ccgagcgtgc cgtcggcccg cagaaagtcg cgcgtgatgc
     7141 cgacgcggaa tttgctcatc gttctccctt gcggcaatct accacacctc gcatgatgag
     7201 tgccccactc aacgagacag ccgcatgacc ttggcccgcg tcatcatcgc cgactttcag
     7261 catggctcgt tggaggtcga gcaagagatt ctcaagggcc tggcggccgt cgccgccctc
     7321 gacgtcacct ccgaggagca gatgtggggc cgcgtcgagg acgccgacgc gatcatggtc
     7381 tatcacacgc tgaaggtgac gcgggccacg atcgagcggc tcacgcggtg ccggctgatc
     7441 gttcgctgcg gcgtcggcta cgacaacgtc gatcacgtat tcgccaagag ccgcggaatt
     7501 ccggtggcca acgtgcccga ctacgggaac gaggaagtcg ccgactcggc gatcgggatg
     7561 atgctcgccc tcacgcgcgg catcaacctg caaaacactg tcttgcggcc cgccggctcc
     7621 aaatggcgat acgacaacgt cgcaccgctc taccgcctcc gcggccgcac gtttgccgtg
     7681 ctcggcctgg gccgcatcgg cacggcggcc gccctgcggg ccaaggccct gggcatgaac
     7741 gtcatctttt acgaccccta caaggccgac ggctacgaca agtccctcag cgtgcggcgg
     7801 gtcgagacgc tcgcggagct gctcggcgag gcgttcgtcc tgagctgcca ttcgccgctg
     7861 acgc
//
LOCUS       DASRJB010000533         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2403067, whole genome shotgun sequence.
ACCESSION   DASRJB010000533 DASRJB010000000
VERSION     DASRJB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403067"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFB80_13850"
     CDS             <1..303
                     /locus_tag="VFB80_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZN34906.1"
                     /translation="FKRDGEACQYCGARPGLDSLTIDHIVPRSRGGTSTWENCVLACI
                     NCNKKKADRTPKEAHMRLHGKPSRPKWQPLYATRHAPVASWAKFISEAYWNVELEP"
     gene            674..1102
                     /locus_tag="VFB80_13855"
     CDS             674..1102
                     /locus_tag="VFB80_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34907.1"
                     /translation="MKRQFRTNDRLSELRAAADADDGQDPRDFFKKESSTRGQGRKTR
                     QLCAQVTQTLEQLLGEAADAQLQALHVVEVAPAPDASQLVVLLASLAGGDVDPGEAEA
                     ALGRAAGWLRSEIAAAIHRKRAPSLIFRILPAASPREVQP"
     gene            1099..2232
                     /locus_tag="VFB80_13860"
     CDS             1099..2232
                     /locus_tag="VFB80_13860"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN34908.1"
                     /translation="MNPPAIARWLAGPLPQDVARSLERLSAAEDVRHIAVMPDVHLSG
                     DVCVGVALATRRLIYPAAVGGDIGCGMAAVAVHAQADMLDERAAAAILSELPRSVPPM
                     RHSRATMPTLAASDTASQLSHPRLAKLVERDGRVQFATLGRGNHFLEFQADQEGRLWI
                     MLHSGSRAMGQAITAHHVALAEQRSQRRKLPWLDAETPEGAAYLADVAWAVQYAAESR
                     LAMLRAAERLLRELFGVSLDWQALIHADHNHVRRETHFGEPLLVHRKGALPAAAGQGG
                     VIPGSMGTASFHVTGRGCEAALCSSSHGAGRALSRSEAGQRIPPRQLQRELRGVWFDQ
                     RLARRLVDEAPLAYKDIRDVMRAQKELTRIDRELRPLISYKGV"
BASE COUNT          371 a          787 c          766 g          372 t
ORIGIN      
        1 ttcaagcgcg acggcgaagc ctgccagtat tgcggcgcca ggcccggact cgattcgctc
       61 acgatcgacc acatcgtgcc ccgctcgcgc ggcggcacca gcacctggga gaactgcgtg
      121 ctggcctgca tcaactgcaa caagaagaag gccgaccgca cgccgaagga agcccacatg
      181 cggctccacg gcaagccctc gcgccccaag tggcagccgc tgtacgccac gcgccacgcc
      241 cccgtcgctt cctgggccaa gttcatcagc gaggcctact ggaacgtcga gctggagccg
      301 taggaaatcg aagccgccac cagtggtcgc ggttcggaat aagtgcggag tgtgaagcga
      361 aagcactgca ttccgcattt ttcgttgatg tcgcctgggc cacgcactct gctcagggtc
      421 cgtttccgtt gcgcgaacct tcttgagacg gctctcgtcc tgaggtaaac ggtggagaat
      481 ctaaccatgc accgcaattc ctctttggcg tccatgagcc tgtaggtctc tcgggaccgg
      541 cgggctcact ctctcacagc ggctttctgc cgcacgattc tttctgcgcg ccgcgggaat
      601 gccgccgcgc cacgccgttt gccttgccgt tcgccactcc ggctgcgctg ccattatcca
      661 atcgagagag aacatgaaac gccaatttcg tacgaatgat cgtctatcag aactccgggc
      721 cgcggcggat gccgacgacg gccaggatcc acgtgacttt ttcaaaaagg agagctccac
      781 tcgcggccaa ggccgcaaga cgcggcaact gtgcgcgcag gtgacgcaaa cgctcgagca
      841 attgctgggc gaagcggccg acgcgcagct ccaggcgctg cacgtggtcg aggtcgctcc
      901 cgcgccagat gcttcgcagt tggtcgtgct gctcgcatcg ctagccggcg gtgatgtcga
      961 tccgggcgaa gcggaagcgg cgctgggccg tgccgccggc tggctgcgca gcgagattgc
     1021 cgcggcgatc caccgcaagc gcgcgcccag cctgatcttc cgcatcctgc cggcggccag
     1081 tccgcgggag gtgcagccgt gaatccgcct gccattgccc gctggctcgc gggaccgctg
     1141 ccgcaggacg tggcccggtc gctcgagcgc ctctccgccg ccgaagacgt gcggcacatc
     1201 gcggtgatgc ccgacgtaca cctgtcgggc gacgtgtgcg tcggcgtcgc gctggccacg
     1261 cggcgcctga tctatcccgc cgctgtcggc ggcgacatcg gctgcggcat ggcggccgtc
     1321 gccgtgcacg ctcaggccga catgctcgac gagcgagcag ctgccgcgat cttgtcggag
     1381 ttgccgcgca gtgttccgcc aatgcggcat tcgcgggcca caatgccgac gctggcagcg
     1441 tcggatacgg cgtcgcagct cagccatccg cggctcgcaa agctggtcga gcgcgacggc
     1501 cgcgtccagt tcgccacgct cggccgcggc aatcacttcc tggaattcca ggccgatcag
     1561 gagggccggc tctggatcat gctgcacagc ggttcgcggg cgatgggcca ggcaattacc
     1621 gcccatcacg tcgcgctcgc cgagcagcgg tcgcagcgcc ggaagctgcc gtggctcgac
     1681 gccgagaccc ccgagggtgc cgcgtatctg gccgacgtgg catgggccgt gcaatacgcc
     1741 gccgaaagcc gcctggcgat gctgcgggct gccgagcggc tgctgcggga gttgttcggc
     1801 gtctcgctcg attggcaggc gctgattcac gccgaccaca accacgtccg ccgcgagacg
     1861 cacttcggcg agccgctgct ggtgcaccgc aaaggagcgc tgcctgctgc ggcagggcag
     1921 gggggcgtga ttccaggctc gatgggaacg gccagtttcc acgttaccgg ccgcggctgc
     1981 gaggcggcgc tgtgctccag ctcacacggc gcgggccgcg ccttgagccg ctcggaggcc
     2041 ggccagcgga tcccgccgcg gcaattgcaa cgcgagctgc gcggcgtctg gttcgaccag
     2101 cgtctcgccc gccggctcgt ggacgaagca cctttggcct ataaagacat tcgcgacgtc
     2161 atgcgggctc agaaggagct gactcggatc gaccgcgagc tgcggccgct gatttcgtat
     2221 aagggcgtat gaatcgtccc gcgagcaggg cggcggcgag cagcagccag ctcgcgccgc
     2281 tgatccatgc gccgcg
//
LOCUS       DASRJB010000534         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4097009, whole genome shotgun sequence.
ACCESSION   DASRJB010000534 DASRJB010000000
VERSION     DASRJB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097009"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFB80_13865"
     CDS             <1..927
                     /locus_tag="VFB80_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34909.1"
                     /translation="QQGQQGQQGQQGQQGQQGQQGQQGQQQQQKQQAQNPARQRLDQA
                     QQKMQDAQKKLEEANRKDSIEDQRAAREELEKAKAELEEILRQLREEEIARTLAALEG
                     RFKKMLEMQLAVYESTRRLDQLPQESRGEPFLVQSGKLSADERKIAVEAQKALTLLQE
                     EGSSIALPTTVDQMHDDMEQVVTRLADAKVDDITIGYEEDIIAALEDIIEALQQAQKD
                     LEEQQQQQQQQQQQPGEQPLVDQIAELKMIKALQERVNKRTVRYSRLLTDDQDPVGRA
                     EAPELADAVKKLSDRQQEIQKITRDIVLGKNQ"
     gene            953..1987
                     /locus_tag="VFB80_13870"
     CDS             953..1987
                     /locus_tag="VFB80_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34910.1"
                     /translation="MRNELTLTLAATILCATGAALAQDELTKRATWSIPATADVKARL
                     DDYLAGKTLDEAAKLKIEALWPDEAQPVDNAELLDRLTASLAVVEPKAAEVVAAVRAG
                     AVPATQPKFAGLSDEKLPPLVRDNLRLLVARWFAQHDLVDESLEILKDTSTDHVADPA
                     TLLFYQAMGQHRLLKKEECLAAVTKLLEREAQLPRRFTTLARLMEADIKPLKVDSLDE
                     IARLMDDIRRRLALARAGKVVRDEEDSVVAKLEKMIEELEKQRQQQQQSGNSGGSQPS
                     NPLQDSQAAQLKGPGEVDPRNLGKKDGWGNLPPKQRQEVMQQIGRELPAHFRETIEEY
                     FKRLAQDGVK"
     gene            2044..>2593
                     /locus_tag="VFB80_13875"
     CDS             2044..>2593
                     /locus_tag="VFB80_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34911.1"
                     /translation="MTTTAISLLALAALAAGPQVTVRPLAGESIAGELAHLAADKVTV
                     QTSAGDKTLSAAELMWVEFPAQKPTEKPPSAWIELLDGSKLLATSFTAAAGKAQITLT
                     SGQTVEAPTRLIRHVRFREQQTPELATQWREIALSMASGDMVVVRKTSMRTVEQAGGD
                     SITLTEQALDQLEGTIHSVGPDS"
BASE COUNT          576 a          834 c          878 g          305 t
ORIGIN      
        1 caacaaggcc aacaggggca acaggggcaa caggggcaac agggacaaca gggacaacaa
       61 ggacaacaag gacagcagca gcagcagaag cagcaggccc agaatccggc ccgccagcgc
      121 ctggaccagg cgcagcagaa gatgcaggac gcacagaaga agctggaaga ggccaaccgg
      181 aaggattcga tcgaggatca gcgggcggct cgcgaggagc tggagaaggc caaggccgag
      241 ctggaggaaa tcctgcggca gctgcgcgag gaagaaatcg cccgcacgct ggccgcactg
      301 gagggccgtt tcaagaagat gctggagatg cagctggccg tgtatgaatc gacccgccgg
      361 ctcgaccagc tcccgcaaga gagccgcgga gagccgttcc tggtgcagtc gggcaagctc
      421 tcggccgacg agcgaaagat cgccgtcgag gcccagaaag ccctgacgct gctccaggaa
      481 gaaggctcct cgatcgccct gccgacgacc gtcgatcaga tgcacgacga catggagcag
      541 gtcgtcaccc gcctggccga cgccaaggtg gacgacatca ccatcggcta cgaggaagac
      601 atcatcgcgg ccctggagga catcatcgaa gccctccagc aggcccagaa ggatctggag
      661 gagcagcagc aacagcagca gcagcagcaa caacagccgg gcgagcagcc gctggtcgat
      721 cagatcgccg agctgaagat gatcaaggcg ctccaggagc gcgtgaacaa gcggacggtc
      781 cggtattcgc ggctcttgac cgacgaccag gacccggtgg gccgggccga ggccccggag
      841 ctggccgacg cggtcaagaa gctgagcgat cggcagcagg agatccagaa gatcacgcgc
      901 gacatcgtgc tggggaaaaa ccaataggcg aatccaacgc agggacgaca ctatgcgaaa
      961 cgaactaacg ctcacgctcg ccgcaacgat cctctgcgcc accggtgcgg cgctggccca
     1021 ggacgagctg accaaacggg cgacctggtc gatcccggcc acggccgacg tcaaggccag
     1081 gctcgacgac tacctggcgg gcaaaacgct cgacgaagcg gcgaagctga agatcgaggc
     1141 cctctggccc gacgaagccc agccggtcga taacgccgag ctgctcgacc gcctgacggc
     1201 ctcgctggcc gtggtcgagc cgaaggccgc cgaggtcgtg gccgccgtgc gggccggcgc
     1261 ggtcccggcc acgcagccga aattcgccgg actgagcgac gaaaagctgc cgccgctggt
     1321 tcgcgacaac ctgcggttgc tcgtcgcccg ctggttcgcg cagcacgacc tggtcgatga
     1381 gtcgctggag atcctgaagg acacctccac ggaccacgtc gccgacccgg ccacgctgct
     1441 cttctatcaa gcgatggggc aacaccgcct gctcaagaag gaggagtgcc tggccgccgt
     1501 cacgaaactg ctggagcgcg aggcccagct gccacggcgg ttcaccacgc tggcccgcct
     1561 gatggaggcg gacatcaagc cgctgaaggt cgattcgctc gacgagatcg cccgcctgat
     1621 ggacgacatc cgccgccgcc tggccctggc tcgcgcgggc aaggtcgtcc gcgacgagga
     1681 agactcggtc gtcgccaagc tggagaagat gatcgaggag ctggaaaaac agcgccagca
     1741 acagcagcaa tcgggtaaca gcggcggcag ccagcccagc aacccgctgc aagacagcca
     1801 ggcagcccag ctgaaagggc ccggcgaggt cgatcccaga aacctcggca agaaggacgg
     1861 ctggggcaac ctgccgccca agcagcggca ggaagtgatg cagcaaatcg gccgcgagct
     1921 gccggcccac ttccgcgaga caatcgagga gtatttcaag cgtttggcgc aggacggcgt
     1981 taagtaacgg gccgccgagc aacgtagaat agggatatcc cgctttccga tggatggtga
     2041 gttttgacga cgacggcgat atccctgctc gcgctggcgg cgctggccgc cggcccgcaa
     2101 gtcacggtca ggccgctggc cggcgagagc atcgccggcg agctcgcgca cctcgccgcc
     2161 gacaaagtca cggtgcagac ttcggcgggc gacaagacgc tcagcgcggc cgaactgatg
     2221 tgggtcgaat tcccggctca aaagccgacg gagaagccgc ccagcgcctg gatcgagctg
     2281 ctcgacggct cgaagctgct ggcgactagc tttacggcgg cggccggcaa ggcccagatc
     2341 acgctcacca gcggccaaac ggtcgaagct ccgacgcggc tgattcgcca cgtgcggttc
     2401 cgcgagcagc agacgccgga gctggcgacc cagtggcgtg aaatcgccct gtcgatggcc
     2461 tccggcgaca tggtcgtggt ccgcaagact tcgatgcgga cggtcgaaca ggcgggcggc
     2521 gattcgatca cgctcaccga gcaggccctc gaccagctgg aggggacgat ccacagtgtc
     2581 ggcccggaca gcg
//
LOCUS       DASRJB010000535         7156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1557241, whole genome shotgun sequence.
ACCESSION   DASRJB010000535 DASRJB010000000
VERSION     DASRJB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557241"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VFB80_13880"
     CDS             complement(<1..955)
                     /locus_tag="VFB80_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN34912.1"
                     /translation="MVTTRPANPTLPASSPPQITECQRLEPAIRVQGVSHAYGSRLAL
                     DDLSLEVSPGELLAVLGPNGGGKSTLFRLLSTLLPLSRGSASVLGYDLRTQAQAVRSV
                     IGVVFQAPSLDRKLTVAENIRLQAALYGLGGRELKTRLEELLDQFALRERARELTERL
                     SGGLRRRVELAKGLIHRPAVLLLDEPSTGLDPAARSDLWQYLRRLKTERGTTIVLTTH
                     YLDEADGADRIAILNEGRLVALGPPDELRASVGGDSITLETNNPDQLAAQIAAQFPLP
                     ARVIDEAVRLETPDGHHWIAKLVEAFPGEINAVRLGKPTLED"
     gene            complement(894..1406)
                     /locus_tag="VFB80_13885"
     CDS             complement(894..1406)
                     /locus_tag="VFB80_13885"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZN34913.1"
                     /translation="MADLAPTDPRYPGHGYDPLEHGDEHGHGHGPSGVGKYVIVFLVL
                     LGLTAVSYLVGNSQTLREKSPGVMWAAMMAVSCAKAMLVILFFMHMLWEANWKYVLTI
                     PASMMSIFLLLMLIPDVGRRVDRYSEERWLYAAQPDSNSHAVAKAAEQHGHDKAGKSD
                     AAGEQPAANH"
     gene            complement(1484..2968)
                     /locus_tag="VFB80_13890"
     CDS             complement(1484..2968)
                     /locus_tag="VFB80_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZN34914.1"
                     /translation="MAHDHDHGHTAVKLEYQPALPIGRGKLCLWLFLSTEIMFFAGLI
                     GTYIVLRFGAPPESWPLPHDVHLQEFIGAFNTFVLICSSVTIVLALEAARGNHAGLSK
                     FWMVATFLLGSVFLGVKAYEYNEKFKHGIFPAKPHSLLYEKADLNYVAAVRQSLAPKS
                     LALTEKLPELTAKRDPLLAKLDAGQALTEQDQQQFTELNRQLLPNDLQYDLVQWTELI
                     AAKDPDQAQRRRALESLAVAIYPLRPLGKEGAEMEHHYVQMLDEEAKTVRHQMQELEK
                     DRSLAQNELAAKKAESDALTAEIAPVQMQRDMIQKEIERLQKAETTTAATEEAPSNLV
                     SLQPPATQPPTANPQVEKLKTELAALDAQLMPKLEAQRALLAEQQRIFEREAAFTTEL
                     KRYQGRVRLLLYTLKAAPHGLNHTFSELRLPMVIPSGNMWASTYFLLTGFHAIHVLVG
                     LIAFVLVLFYKLDRTKAHILENVGLYWHFVDLVWIFLFPLLYLF"
     gene            complement(3002..3910)
                     /gene="cyoE"
                     /locus_tag="VFB80_13895"
     CDS             complement(3002..3910)
                     /gene="cyoE"
                     /locus_tag="VFB80_13895"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN34915.1"
                     /translation="MSTSPLTLAAPRPLLLAKALDYLELTKPKIAVLELVVVLAAGFV
                     ATWGQPHPALLLHVMVGTLLIAASASAANQWLERYRDGRMARTASRPLPAGRLSAGEA
                     LAFAAVTLLAGSLYLSAFVNSASLGWALLTWVVYVLAYTPLKTVSPANTAVGAVAGAL
                     PVLIGWSATGAAIDARAVALALVLFLWQFPHFMAIAWLYRRDYGAAGYQMLTVVDPTG
                     RRAGAQAVLAALALVPVSLVPVLSAAGVGSALYAAGAILLGGGQLVAAGLFARRPDEF
                     SARLLLRASLAYLPTLLVTLALAPWI"
     gene            complement(3907..4941)
                     /locus_tag="VFB80_13900"
     CDS             complement(3907..4941)
                     /locus_tag="VFB80_13900"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZN34916.1"
                     /translation="MPNRNEPRPSPWPHRLAVTLALVTFPLIWVGGLVTTYDAGMAVP
                     DWPGTYGYNLLLYPWQSWLAAPWDLFIEHGHRLLGSAAGLVSIVLLAVVLLVDRRRWL
                     LLAAIGALALVIVQGVLGGARVLFDERLVALLHGCVGPLFFAYVGALAVATSKPQDAS
                     VKESAAGRNLLRAAAVTAALAYVQLVLGALVRHVPLTASPQVFRAALLLHLVVGGVLA
                     VQIVLTAARAWSARAAGGVAGPGLLLPVLVAVQIALGLATYVAKYSWPAWLGDYAFAA
                     SFVVQEKSLAQSLITTAHVAGGSLILFTAVVLAARSVRLFGLKAAISGHALDAGTSAA
                     IGRRLEWRAV"
     gene            complement(4961..6766)
                     /locus_tag="VFB80_13905"
     CDS             complement(4961..6766)
                     /locus_tag="VFB80_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN34917.1"
                     /translation="MSAISLPAPAHEAHGGHTMSVGEFINTYVFSRDHKIIGIQFLFS
                     TLLWFLVGGLLALGIRWQLAWPWSNMPVIGRMLFSAEGGQISPEFYTTLFTMHATVMI
                     FFVIIPILAGAFGNYLIPLMIGADDMAFPTLNMLSYWFMWPAFICIGVSFLWPPYGAG
                     GGWTSYPPLSAVSEAAPGSGFPQTMWLLGITCVGISSMLGSVNYMTTIIQMRAPGMTM
                     FRLPLTIWAMFITAILQAFALPVLTAAGFMQLADRLLGTGFFLPEGLIVNNSLPASGG
                     GQPLLWQHLFWFYSHPAVYIMILPAMGMVSDIIACFARKPIFGYKPMVYSISGIAGLG
                     FIVWGHHMFVSGMNPLLGMTFMVSTMMIALPSAVKVFNWLGTVWGGKVQYTTPMLFAL
                     SFVSMFIIGGLSGIFMAATPVDIFIHDTYFIVAHFHYVLFAGTAMAVFGAIYMWFPKM
                     FGRMMDENLGKVHFFLTFIFLNGTFYTMHILGAVGFPRRLADPYDYETFRHLLPMNQF
                     MSICAILMVATQVIFAYNFIRSMFLGTPVGRNPWGANSLEWFAPSPPGHGNFDMQPIV
                     YRGPYEYGSPEVDTDNYPQWQPPPTDPKRRYKPEH"
     gene            complement(6763..>7156)
                     /locus_tag="VFB80_13910"
     CDS             complement(6763..>7156)
                     /locus_tag="VFB80_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZN34918.1"
                     /translation="RYAHTKGKDWKAGDSYEFGKDDVFIVNDLHLPQNEEIVLAIKSM
                     DVLHSFFLPNLRVKQDVVPGMKQYVWFQANKSGVYDIVCAELCGWGHYKMRGRLTVEP
                     RDTYDAWLAQQSAEQNRAGFTPSPGAAE"
BASE COUNT         1365 a         2234 c         2392 g         1165 t
ORIGIN      
        1 cgtcctccag agtcggcttg cccaggcgca cagcattgat ctcgcccgga aaggcctcca
       61 ccagtttggc aatccagtga tgtccgtccg gcgtttctag ccgcacggct tcgtcgatga
      121 ctcgcgcggg caaggggaac tgcgcggcga tttgcgcggc gagctgatcg ggattgttcg
      181 tttccagcgt gatcgaatcg cctccgacgc tggcccgcag ctcgtccggc ggaccgaggg
      241 ccacgagccg gccttcgttc agaatcgcga tccggtctgc gccgtcggct tcgtccaagt
      301 agtgcgtggt gaggacgatc gtcgtgccgc gctcggtttt cagccgccgc aaatactgcc
      361 acaagtcgct gcgggccgcc gggtcgaggc cagtactcgg ctcgtcgagg agcagcacgg
      421 ccggccgatg gatcaggccc ttggccagct ccacgcgccg ccgcagtcca cccgacagcc
      481 gctccgtcaa ctcgcgggcc cgctcgcgga gggcgaactg gtcgagcaat tcttccaggc
      541 gcgttttcag ctcgcgtccg cccaggccgt agagggcggc ttgcagccgg atgttctccg
      601 ccacggtgag cttgcggtcg aggctgggcg cttgaaacac aacgccgatc acgctgcgca
      661 cggcctgggc ctgcgtccgc agatcgtagc cgaggacgct cgccgagccg cgcgacagcg
      721 gcagcagcgt cgagagcaag cggaacaatg tggacttgcc gccgccgttg gggccgagca
      781 cagccagcag ctcgcccggg ctgacttcga gcgagagatc gtccagcgcc agccgcgagc
      841 cgtaggcgtg gctcacgccc tgcacgcgga tcgccggctc cagccgctgg cactcagtga
      901 tttgcggcgg gctgctcgcc ggcagcgtcg gatttgccgg ccttgtcgtg accatgttgc
      961 tcggcagcct tggctaccgc gtggctgttg gaatcgggct gggcggcgta gagccaccgc
     1021 tcctcggaat atcgatcgac gcggcggccc acgtcgggaa tgagcatcag gagcaggaag
     1081 atgctcatca tgctggcggg gatcgtgagc acgtatttcc agttggcttc ccagagcatg
     1141 tgcatgaaga acaggatcac cagcattgcc ttggcgcagg agacggccat catcgcggcc
     1201 cacatcacgc cgggcgactt ctcgcgcaga gtctggctgt tgccgacgag atacgaaacc
     1261 gccgtaagtc ccagcagcac gagaaacacg atgacgtact tgccgacgcc gctcggtcca
     1321 tggccgtggc catgttcgtc gccgtgctcg agcggatcgt agccgtgccc gggatatcgc
     1381 gggtcggtgg gggcgaggtc ggccataaaa agggttcagg gttggaggtt caggattcag
     1441 gtcagcatcg ctgaccactc actaaatcag cactcacttg cgcctaaaac aggtacagca
     1501 gcgggaagag gaaaatccac accagatcga cgaagtgcca atacaggcca acgttttcca
     1561 ggatgtgggc cttcgtccgg tcgagcttgt agaacaggac caggacgaag gcgatcaggc
     1621 cgactagcac gtgaatcgcg tgaaagccgg tgagcaggaa gtaggtgctg gcccacatgt
     1681 tgccgctggg gatgaccatc ggcagccgca attccgagaa cgtgtggttc aggccgtgcg
     1741 gagcggcctt gagcgtgtag agcagcagcc gcacgcggcc ctggtagcgc ttgagctcgg
     1801 tggtaaaagc ggcctcgcgc tcgaagatgc gctgttgttc agccagcagg gccctttgcg
     1861 cttcgagctt gggcatgagc tgcgcgtcga gggcggccag ttcggtcttg agcttctcga
     1921 cctgcggatt ggcggttggc ggctgcgtgg cgggcggctg gagcgagacc aggttggatg
     1981 gtgcctcctc ggtcgccgca gtcgtcgttt cggccttttg cagtcgctcg atctccttct
     2041 ggatcatgtc gcgctgcatt tgcacggggg cgatctcggc ggtcagcgcg tcggattcag
     2101 ccttcttagc cgcaagctcg ttctgcgcga ggctgcggtc cttttccaac tcctgcatct
     2161 gatgccggac ggtcttcgcc tcctcgtcga gcatctggac gtagtggtgc tccatctccg
     2221 cgccctcctt gccgagcggg cggagaggat agatggccac ggcgaggctt tccagcgccc
     2281 ggcggcgctg cgcctgatcg gggtccttgg cggcgatcag ctcggtccac tgcaccaggt
     2341 cgtactgcaa gtcgttaggc agcagctgcc ggttgagctc ggtgaattgc tgttgatctt
     2401 gctccgtgag cgcctgtccg gcgtcgagct tggccagcag cggatcgcgc ttggccgtca
     2461 gctcgggtag cttttccgtg agggccaggc tcttgggggc gagactttgc cgcacggcgg
     2521 cgacgtaatt caggtccgcc ttttcataaa gcaggctgtg cggcttggcg ggaaaaatgc
     2581 cgtgcttgaa tttctcgttg tactcgtagg ccttgacgcc gagaaacacg ctgcccagga
     2641 ggaacgtcgc caccatccag aatttggaca ggccggcgtg gttgccgcgg gccgcttcca
     2701 gggccagcac gatcgtcacg ctcgagcaga tcagcacgaa cgtgttgaac gccccgatga
     2761 actcctgcag gtgcacgtca tgcggcagcg gccagctttc cggcggcgcg ccgaagcgga
     2821 gcacaatgta ggtgccgatc aggcccgcaa agaacatgat ctccgtcgag aggaagagcc
     2881 acaggcagag cttgccgcgc ccgatcggca gcgcgggctg atactccagc ttgacggcgg
     2941 tgtgtccgtg atcgtgatcg tgtgccatct tcggttcttc agttctttgg tacttcgact
     3001 ttcaaatcca gggcgcgagc gccagcgtga ccagcagcgt gggcaagtaa gccagcgacg
     3061 cccgcagtag caatcgcgcc gaaaactcgt cgggccgccg ggcgaacagt cccgccgcaa
     3121 ccagctggcc gccgcccagc aggatcgccc ccgcggcgta cagcgcgctg cccacgccgg
     3181 ccgcagaaag caccggcacg aggctgactg gaacgagcgc cagcgccgcc aataccgcct
     3241 gagcgccggc ccggcggccg gtcggatcga cgacagtcaa catctggtaa cccgccgcgc
     3301 catagtcgcg gcggtacagc caggcaatcg ccatgaaatg cggaaactgc cagaggaaca
     3361 gcaccagggc cagggcgacg gcccgcgcgt cgatggccgc gccggtcgcc gaccagccga
     3421 tcagcaccgg cagcgcgccc gccaccgcgc cgacggcggt attggcgggc gagaccgtct
     3481 tcagcggcgt ataggccagc acatagacga cccacgtcaa gagggcccag cccaggctgg
     3541 cggaattgac gaaggcggac aggtagagcg agcctgccag cagcgtgacg gcggcgaaag
     3601 cgagcgcctc gcccgcggag agccgtcccg ccggcagcgg acgcgaggcc gtgcgggcca
     3661 tccgcccgtc gcgataccgt tccagccact ggttggctgc gctggcgctg gccgcgatca
     3721 gcaatgtgcc gaccatcacg tgcagaagca gcgcgggatg cggctggccc caagtcgcta
     3781 cgaaaccggc cgccaaaacg acgaccagtt ccagcacggc gatcttcggc ttggtcagtt
     3841 ctaagtaatc gagcgccttg gccaacagta gcggccgcgg ggcggcaagg gtgagggggg
     3901 acgtgctcat acggccctcc actcaaggcg gcgaccaatc gccgcgctgg tgccagcgtc
     3961 aagagcgtga ccactgatcg cggctttgag accaaacagc cgcacggacc gcgcggcgag
     4021 cacgacggcg gtgaacagaa tcagcgagcc acctgcgacg tgggcggttg taatcagcga
     4081 ttgagcgagg ctcttttcct gcacgacaaa cgaagcggcg aaggcgtaat ctcccagcca
     4141 cgccggccag gagtacttgg caacatacgt ggccagtccc agtgcgattt gcaccgcaac
     4201 cagcacgggg aggagcaggc ccggcccggc gacgcccccc gcggctcgcg cagaccaggc
     4261 ccgagcagcg gtcaggacga tctgcacggc cagcacacca ccgacgacca gatgcagcag
     4321 caaggcggcg cggaacactt gcggggacgc cgtcagcggc acgtgccgca ccagcgcgcc
     4381 cagcacgagt tgcacatagg ccagcgctgc cgtcactgcg gcggcccgta gcaggtttcg
     4441 gcccgcggcc gactccttca cagacgcatc ttgcggcttg ctcgtcgcga cggcgagcgc
     4501 tccgacatat gcaaagaaca gaggtccgac acagccgtgg agcagtgcga ccagccgttc
     4561 gtcgaacagc acgcgagcgc cgccgagcac gccttggact atgactagcg ctagagctcc
     4621 gatagcagcc agcagcagcc agcggcggcg atcgacgagc aggacgacgg ccaagagcac
     4681 gatcgaaacc agccctgcgg cgctacccag caaccggtgg ccgtgctcga tgaacaggtc
     4741 ccaaggtgcc gccagccagg actgccaggg atagagcagc aggttgtagc cgtaggtgcc
     4801 gggccaatcg ggcacggcca tgccggcgtc gtaggtggtg acgagtcccc cgacccaaat
     4861 cagcgggaag gtgaccagcg caagggtaac cgccagccgg tgaggccaag gggacggtcg
     4921 cggctcattg cggttgggca tcgttcaagt acagggccga ctagtgttcg ggcttgtagc
     4981 gccgcttggg gtcggtgggc ggcggctgcc actgcggata gttgtcggta tcgacctcgg
     5041 ggctgccgta ttcgtatggg ccgcggtaaa cgatcggctg catgtcgaag ttgccgtgcc
     5101 cgggcggact gggagcaaac cactccaggc tgttggcgcc ccacggatta cggccgacgg
     5161 gggtgccgag gaacatgctg cggatgaagt tgtaggcaaa gatcacctgc gtggcgacca
     5221 tcagaatcgc gcagatgctc atgaactggt tcatgggcaa caggtgccgg aaggtctcgt
     5281 agtcgtacgg gtcggccagg cggcgcggaa aaccgacggc gcccagaatg tgcatcgtgt
     5341 agaacgtgcc gttgaggaag atgaacgtca ggaagaaatg gaccttgccc aggttctcgt
     5401 ccatcatgcg gccgaacatc tttggaaacc acatgtagat cgcgccgaac acggccatcg
     5461 ccgtgccagc gaagagcacg tagtggaagt gggccacgat gaagtaggtg tcgtggatga
     5521 agatgtcgac cggcgtggcc gccatgaaaa tacccgacag gccgccgatg atgaacatcg
     5581 acacgaacga cagggcgaac agcatgggcg tggtgtattg cactttgcca ccccagaccg
     5641 tccccagcca gttgaacacc ttcacggcgc tcggcagggc gatcatcatc gtgctgacca
     5701 tgaacgtcat gcccagcagc gggttcatcc ccgacacgaa catgtggtgg ccccacacga
     5761 tgaagcccag cccggcgatg ccgctgatcg aatagaccat cggcttgtag ccgaagatcg
     5821 gcttgcgggc gaagcaggca atgatgtcgc tgaccatgcc catcgccggc aggatcatga
     5881 tgtacacagc cgggtgcgag tagaaccaga acaggtgttg ccaaaggagc ggctgcccgc
     5941 cgccgctggc cggcagcgag ttgttgacga tcagcccctc gggcaggaag aagccggtgc
     6001 ccagcaagcg gtcggccagc tgcatgaagc cggcggccgt cagcacgggc agggcgaagg
     6061 cttgcaaaat ggccgtgatg aacatcgccc agatcgtcag cggcaggcga aacatcgtca
     6121 tgccgggagc ccgcatctga atgatggtgg tcatgtagtt gaccgagccg agcatcgacg
     6181 aaatgccgac gcaggtgatg cccagcagcc acatcgtctg tggaaagccg ctgcccgggg
     6241 cggcctcgct gacggccgag agcggcggat agctggtcca gccgccgccg gcgccgtagg
     6301 gcggccagag gaagctgacg ccgatgcaga tgaacgcggg ccacatgaac cagtagctga
     6361 gcatgttcag cgtcgggaac gccatgtcgt cggcgccgat catcagcggg atcaggtaat
     6421 tgccgaacgc gccggcgagg atcgggatga tcacgaagaa gatcatcacc gtggcgtgca
     6481 tcgtgaacag cgtggtgtag aactcgggcg aaatctgccc gccttcggcc gagaacagca
     6541 tccggccgat caccggcatg ttcgaccacg gccaagccag ctgccaacgg atccccaggg
     6601 ccagtaggcc tccgaccaga aaccacagca gcgtagaaaa caggaactga atcccaatga
     6661 tcttgtggtc gcgcgagaac acgtaggtgt tgatgaactc gccgaccgac atcgtatggc
     6721 cgccgtgcgc ctcgtgggcg ggggcgggca ggctgatcgc actcattccg cggctcctgg
     6781 gctgggtgtg aatcccgcgc gattctgctc ggcggattgc tgggccagcc acgcgtcata
     6841 tgtgtcgcgc ggctccaccg tcagacggcc gcgcatcttg taatgtcccc agccgcacaa
     6901 ctcggcgcag acgatgtcgt agacgccgct cttgttggcc tggaaccaga cgtactgctt
     6961 catgccgggt acgacatcct gcttcacccg caggttgggc agaaagaagc tgtgtagcac
     7021 gtccatgctc ttgatggcca ggacgatctc ttcgttctgc ggcaggtgca ggtcgttgac
     7081 gatgaacacg tcatccttgc cgaattcgta cgaatcgccg gccttccagt ccttgccctt
     7141 ggtgtgggca tagcga
//
LOCUS       DASRJB010000536         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3249427, whole genome shotgun sequence.
ACCESSION   DASRJB010000536 DASRJB010000000
VERSION     DASRJB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249427"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFB80_13915"
     CDS             <1..1122
                     /locus_tag="VFB80_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34919.1"
                     /translation="GLDQPDYVLLRAPRPTIISSTTGDFFDIQGSWDNFRQAKRFYGT
                     LGYPERVDLVEAPGGHGVQAGNLTGIVRWMRRWLLNKDDAVTVDKVETRKEAELLCTE
                     KGQVLLLPGERSVFDLNVEIEERLAAGRKEYWQKTPKEEALAAVRKRAGVRKLADMQP
                     PTHTDIGRVQRDGYHIDKLTINTASGVPLPALTYHPKTPQDDAYLYLHEDGKAADGAV
                     GGPIEKLVHEGYVVVAVDLRGIGETASGKPDAMLGDWKHFYTAYLLGQSLVGLHTEDA
                     LAAGHFVAYYKTKKPRRVHLVGVGQAGIPALHAAALGDDLFASLALRGAPAAWSPLVG
                     QRVPARQLASTVHGALGLYDLPDLVRAFEPRKVYVESAQ"
     gene            1103..2809
                     /locus_tag="VFB80_13920"
     CDS             1103..2809
                     /locus_tag="VFB80_13920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN34920.1"
                     /translation="MSSRPSEPASSDSSPRLEEAVAAYLERLDSGATPDREAFLKQYA
                     DVAEGLAEFFADHDQMNSLAQPLREQGSTSPGLDPTITAPPHSPQASAAGEPPLRIGP
                     YEIETELGRGGMGVVYRARHSELRRNVALKTLLIGRLASREDRTRFAAEALAAAKLDH
                     SGIVPIFDVGESEGQPYFAMPLIVGLSLAERLREEGPLAPHTAARLLRKIVAAVAYAH
                     AHGVVHRDLKPGNILLAEGESSTSGELSGVGDPKITDFGLAKRMGDEAHLTASGQILG
                     TPSYMPPEQAAGKLNEVGPAADIYALGAILYATLTGRPPFQAESPVEVILQVLEREPP
                     LPSSLARGVPRELEWICLKCLEKRAADRYATADELLADLDRFLRHEPPEARRATPIAH
                     FRRWVRRRPVLAGHVIGLGVPLAVAQIVFVSHPEREWAYHLRICGVLVAWIAASFVFQ
                     WLMDRKRTKDWPLYVWCSADAALLAGLLYFVKSPLGAFFGSYLMLIAAAGLAGSTRLV
                     AWTTFCCCLSYLLLLAVHPEEKQPLHYIALALVNFALVGTAVGYQVWRMSVLREYYGE
                     RP"
     gene            complement(2806..3615)
                     /locus_tag="VFB80_13925"
     CDS             complement(2806..3615)
                     /locus_tag="VFB80_13925"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HZN34921.1"
                     /translation="MPIEVTCQSCSGQFRLPDSAAGKKIRCPKCKRPIEVPAAKAPVF
                     SMAMDETSVLPPAPLPPAPLPPAPLPPAPKTPVPLPPAPKPPAVEWRLKTADGEEYGP
                     VPRSELDDWFREGRITAECQILGGLGQQWQPAADVFQLNGAAERVAPQAAADDDAEEG
                     PSDPLAAEIEQPLPNFQAAPTKSPARSKKAAPAAGAAVSADRSQRSRIVAGFLGVFLG
                     PLGIHRFYLGYWGVGLAMLFTGGGLVLWSLIDSIFVMVGRVPDADGRPLSD"
     gene            complement(3625..>3985)
                     /locus_tag="VFB80_13930"
     CDS             complement(3625..>3985)
                     /locus_tag="VFB80_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN34922.1"
                     /translation="ISKKLYDLTTAGRLDEARDLQYRLLPLFDTMLYSAEFPEGFRAG
                     LQLRGFNTGRGRQPQSLVQQQSLVKLKDQIQCLLAAEGFTSEPLGGCPAGESIDPEHV
                     ARIVQGVVGELKRRGMA"
BASE COUNT          635 a         1344 c         1382 g          624 t
ORIGIN      
        1 ggcctcgatc agccggacta cgtgctgctg cgtgccccgc ggccgacgat catcagctcg
       61 acgaccggcg acttcttcga cattcaggga agctgggaca acttccgcca ggccaagcgc
      121 ttctacggca cgctcggtta tcccgagcgg gtcgatctgg tcgaagcgcc gggcggccat
      181 ggcgtgcagg cgggaaatct caccgggatc gtgcggtgga tgcggcggtg gctgctcaac
      241 aaagacgacg ctgtcacggt ggacaaggtc gagacccgca aagaggcgga gctgctttgc
      301 accgagaagg gacaggtcct gctgctgccc ggcgagcggt cggtcttcga cctgaacgtg
      361 gagatcgaag agcgcctggc ggccggccgg aaggaatact ggcagaagac gcccaaggaa
      421 gaagcgctcg cggcggtgcg gaagcgtgcc ggcgtccgca agctggccga tatgcagccg
      481 ccgacgcata ccgatatcgg ccgcgtgcag cgcgacggct atcacatcga caagctgacg
      541 atcaacacgg ccagcggcgt gccgctgccg gcgctcacgt atcacccaaa gacgccgcag
      601 gacgacgcct atctgtatct gcacgaagac ggcaaggcgg ccgacggcgc ggtcggcggg
      661 ccgatcgaga agctcgtgca cgagggctat gtggtcgtcg cggtcgatct gcgcggcatc
      721 ggcgagacgg ctagcggcaa gcccgatgcg atgctcggcg attggaagca cttctatacg
      781 gcgtatctgc tcgggcagtc gctcgtcggc ctgcacacgg aggatgctct ggccgcgggc
      841 cacttcgtcg cctactacaa gacgaagaag cctcgccgcg tgcacctggt cggagtgggc
      901 caggcaggca ttcccgcgtt gcacgccgcg gccttgggcg atgacctgtt cgcatcgctc
      961 gccctgcgcg gcgcgccggc ggcgtggtcg ccgctcgtcg gccagcgtgt gccggcccgg
     1021 cagctcgcct cgacggtcca cggcgcactg ggcctctacg atctgcccga cctcgtccgc
     1081 gccttcgagc cgaggaaggt ctatgtcgag tcggcccagt gaaccggcca gcagcgattc
     1141 ctcgccgcga ttggaggagg ctgtcgcggc ctacctggag cggctcgaca gcggcgcgac
     1201 gcccgaccgc gaggcgtttt tgaaacagta cgccgacgtg gccgaagggc tggccgagtt
     1261 ctttgccgac cacgaccaga tgaactcgct cgcgcagccg ctgcgcgaac aaggcagcac
     1321 atcgcccggt ctcgatccga cgatcaccgc gccgcctcac tcgccacaag ccagcgccgc
     1381 gggcgagccg ccactgcgaa tcggcccgta tgaaatcgag acggagcttg gccgcggcgg
     1441 catgggcgtc gtttaccgcg cgcggcacag tgagctgcgg cggaacgtgg cgctcaagac
     1501 gctgctcatc ggccggctgg cctcgcgcga agaccgcaca cggttcgcgg ccgaagccct
     1561 ggccgcggcg aagctcgacc acagcggaat cgtcccgatc ttcgacgtcg gcgagtccga
     1621 aggacagccc tacttcgcga tgccgctcat cgtcggcctc agcctggccg agcggctgcg
     1681 cgaagagggt ccgctcgcgc cgcacactgc ggcccggctg ctccgcaaga ttgtggcggc
     1741 cgtcgcctac gctcacgcgc acggcgtcgt acaccgcgac ctcaagccgg ggaacatcct
     1801 gctggccgaa ggcgagtcga gcaccagcgg cgagctgtcc ggcgtcggcg atccgaagat
     1861 caccgacttc ggcctggcca agcggatggg ggacgaggct cacctcacgg ccagcgggca
     1921 gattctcggc acgccgagtt acatgccgcc cgagcaggcc gccggcaagc tcaacgaggt
     1981 tggcccggcg gcggacattt atgcgctggg agcgatcttg tacgcgacgc tgacggggcg
     2041 gccgccgttt caagcggaga gcccggtgga agtgatcctg caggtattgg agcgcgaacc
     2101 gccgctgccc agctcgctgg cgcggggcgt gccgcgggaa ttggagtgga tttgcctcaa
     2161 gtgtttggag aagcgggcag ccgaccgcta cgcaacggcc gatgagctgc tggccgacct
     2221 cgaccgcttt ctccgccacg agccgccgga ggcccgccgg gcgacgccca tcgcccactt
     2281 tcgccgctgg gttcgccggc ggccggtgct ggcgggccac gtcatcggcc tgggcgtgcc
     2341 gctggcggtg gcccaaatcg tgttcgtctc gcacccggag cgcgagtggg cgtatcacct
     2401 gcggatctgc ggcgtgctgg tggcctggat cgcggcgagc ttcgtcttcc agtggctgat
     2461 ggaccgcaag cgcacgaagg actggccgct ctacgtgtgg tgctcggccg acgcggcgct
     2521 gctcgccggc ctgctctact tcgtcaaatc gccgctcggt gcgttcttcg gcagctacct
     2581 gatgctgatc gccgccgccg gactggcggg cagcacgcgg ctggtcgcct ggacgacgtt
     2641 ttgctgttgc ctgagctact tattgctcct cgctgtccat ccggaggaaa agcagccgct
     2701 gcactacatt gccctggccc tggtgaactt cgcgctggtc ggcacggcgg tcggctatca
     2761 ggtctggcgg atgagcgtgc tgcgggagta ctacggcgag cggccttagt cgctgagcgg
     2821 gcggccgtcg gcgtcgggaa cgcggccgac catgacgaag atcgaatcga tcagcgacca
     2881 cagcaccagg cctccgccgg tgaacagcat cgccaggccg acgccccagt agccgagata
     2941 gaaccggtga attcccagcg ggccgagaaa cacgcccaaa aaaccggcga cgatccgtga
     3001 ccgctgactg cggtcggcgc tcactgccgc gccggccgcc ggcgcggcct tcttcgaccg
     3061 cgccggactc ttcgtcggcg cggcttggaa attcggcagc ggttgctcga tttccgcagc
     3121 aagcggatcg gagggccctt cttcagcatc gtcgtcggca gccgcctgcg gtgcgactct
     3181 ttccgccgcg ccgttcagtt gaaagacgtc cgccgctggc tgccactgtt ggccgaggcc
     3241 gccgagaatc tggcactccg cggtgatgcg gccctcgcgg aaccagtcgt ccagctcgct
     3301 ccgcggtacc gggccgtatt cctcgccgtc ggcggtcttg aggcgccact caaccgccgg
     3361 gggcttcggc gcaggcggca agggcacggg cgtcttgggc gccggcggca ggggcgcagg
     3421 cggaaggggc gccggcggca atggcgccgg cggcaacacg ctcgtctcgt ccatcgccat
     3481 cgagaagacc ggcgctttcg ccgccggtac ttcgatcggc cgcttgcact tgggacagcg
     3541 gatcttcttg cccgccgccg aatcgggcag gcgaaactgg ccagagcagc tctggcacgt
     3601 gacttcaatc ggcatagggt gccattatgc catcccgcgg cgcttcagtt caccgacgac
     3661 cccctgcacg atccgggcca cgtgctccgg gtcgatcgat tcgcccgcgg ggcagccgcc
     3721 gagcggctcg ctagtgaagc cttcagccgc gagcaggcac tgaatctggt ccttgagctt
     3781 gaccaggctc tgctgctgca ccagagactg cggctgccgg ccgcggccgg tgttgaagcc
     3841 gcggagctgc agcccggccc gaaaaccctc ggggaactcg gccgagtaga gcatcgtgtc
     3901 gaagagcggc agcaggcggt actgcaagtc gcgggcttcg tccaggcgac cggccgtcgt
     3961 caggtcgtag agcttcttcg agatc
//
LOCUS       DASRJB010000537         6465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1557499, whole genome shotgun sequence.
ACCESSION   DASRJB010000537 DASRJB010000000
VERSION     DASRJB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557499"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFB80_13935"
     CDS             <1..1160
                     /locus_tag="VFB80_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34923.1"
                     /translation="LTIEDRRLKVADDGRTLAGPEGAPVRVATRLKRDEFLAWCLDRI
                     LSHGTPTWPAPPGNLAQLVEQGGFPSRVHVVEDYDTDIEKRWWLCGKLESQDLPPGSR
                     RAFRAVLTQDFDDRQGNRNTSYRAVVFNPVPGPPMGASTRLSFRYKLRGTDRLRVQLF
                     SLSNGYHRYLSLAGLPQDAWRSMAVDMTQMRRPDGTGGPLAADERIDDIQFYIDPRAE
                     LLIDDVILYDASAADEKRPFPQRILFTGWFDTGKQGQEWPGEFEIVPHEKPRTWKHAR
                     SIAGEGGQQRLVVDLRGPRRLSPVTALTFHYRLRGADRLRIELADRRGGTSIAGTAAD
                     LKRDAWSETTVPFEVPAAADKEAFATEIRFLPQAGAVLEIDNLLLYQPAGE"
     gene            1281..3710
                     /locus_tag="VFB80_13940"
     CDS             1281..3710
                     /locus_tag="VFB80_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN34924.1"
                     /translation="MVMLLAGCWGPQSPPPAPPAVASGGQQSAAETLPSKPPAEWTAG
                     EILQRLLATYRSAQTYQDQGVVRLAYRQDGQPVSREEPARVAFERPGKLSLAAYQAIV
                     KCDGQSLRARIEDPDSRNIDGQVLVRPAPRELKLTDLAGDALLYDTISSELRRQPIQL
                     ELLLESSGLVSAFGADVACRRLEDETHEARPCFRVEVPSPGGAFVFWVDQAQWLLRRL
                     DYPAAALLPALANDPAVSDLQLLADMRGATIGATIPAEQFVLEIPAGAKRMKSFVIPP
                     PPLPSNLFGRQPPEFSFVDLEGKAVGAKELAGKPAVLVWYHDNPACEATLQQVSLARQ
                     RLKDSAALLAVATDPTTTDNAAVARRLAEWKVELPVARDLEAFGDKAFRIQVQPTIVV
                     LDPQGRVQIYQPGGNPELADQLVTIVQRLSRGDDLATELLERRAREQQQYEQLVAKGG
                     EEPSQVVELPEAVIRRRTEPKQLKLQPLWTCSELKSPGNLLVVESEGQPPRIFVFEGW
                     RTIAEVDAQGKIAGRHALAIPERAAVALLRTATAKDGQRYFAVSAPLAPQVFVFDGDW
                     KLVLTHPPPGETPLAVLDLAFADLGQADGTPDLLVASVADQGLAAVSLKGEQLWRNRA
                     FPNVVSAAVTLPNDAGSWGILLTGESGAILRVNGYGHEDPPAQVAGRQVLSLIAGRFP
                     AAKQAALLALAGGERGERLAVGLTSNLKEAWNYPLPAGVHARPIEPVAPGRVLPGREG
                     EWWLAAPDGSIHVVSEDGDFFDSFYYGEVLTGLAAAKLNQRPALLIATDAGLSAWEVA
                     APLPRPATRER"
     gene            3852..4589
                     /locus_tag="VFB80_13945"
     CDS             3852..4589
                     /locus_tag="VFB80_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34925.1"
                     /translation="MRMSQRWVMLCSLALVVALTASAAAQQRRGGFGFGFGRAPQGLV
                     SLAAQEPVQKDLGLSADVVGKISPLNDEYRAAIQKEMQAAGTLDFQGLRDLPEAERAA
                     KLAEYTKKTTEASAKVNTEYTPKLQAIVGADSIKRLKQIQIQAQGSEALATADVAAEL
                     KLTDDQKKKLADLATEYTAKQRELFNPGTDQQEARAKRAELNKERDTKAEALLTAEQK
                     SKYTALKGPAFDVSTLSTGRGRRGNNN"
     gene            4800..5039
                     /locus_tag="VFB80_13950"
     CDS             4800..5039
                     /locus_tag="VFB80_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34926.1"
                     /translation="MRQSTISRRELRSSDVRGSMRDARRQRIRDAWSPAERSARAEAA
                     QQRREDLARLLFGAPALEIWAVGAPGDDDLARLAG"
     gene            complement(5190..>6465)
                     /locus_tag="VFB80_13955"
     CDS             complement(5190..>6465)
                     /locus_tag="VFB80_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN34927.1"
                     /translation="LIFFKGVEIMKSSWNKWLACGLVAASAVWAGPWASGQSVPVQVR
                     RPRVVEEVDGDFLIRLAEGGAGEAEDKDALPTSDYWIGIALGGELPAIVRQQLGLEHG
                     LAVADVMADSPAAKAKFQTNDILIKAGDANLKGAADLIKAVDAAKERELAIIILRGGK
                     EQTIKVAPIKRPKTEADSGSGSNFSLRAVAPDVQAEIKKLEEALSQLKDKTGAAPLGL
                     MLARPAVVPPTVAGKYYSVLNSTDLPKDVTISITKKGDEPAKIHVEKDGKTWDVTEEK
                     LGDLPEDLRSHVQQFLGHRLSLKLPGAVGGSGPNTIRYGYGRTPAAPGTPVPAARPVP
                     PAPPAPPASPRPPQAVAPPIPAQPQYAPLRTYRLETAGGGGVEGKLDAIMKKLDQLES
                     KSVEQLEKEVKQLRKELDELRGKSPGEKAKGE"
BASE COUNT         1052 a         2194 c         2155 g         1064 t
ORIGIN      
        1 tcctcacgat cgaagaccgc cgcctgaagg tcgccgacga cggccgcacg ctcgctggcc
       61 cggagggagc gcccgtccgc gtcgcgacgc gcctcaagcg cgatgaattc ctcgcctggt
      121 gcctggatcg catcctgtcg cacggcacgc cgacctggcc cgcgccgccg gggaatctgg
      181 cgcagctcgt ggagcagggc ggctttccca gtcgcgtgca cgtcgtcgag gactacgaca
      241 ccgacatcga aaaacgctgg tggctgtgcg gcaagctgga atcgcaggac ctgccgccgg
      301 gcagccggcg ggcgtttcgc gccgtgctga cgcaggattt cgacgaccgc cagggcaacc
      361 gcaacacgag ctatcgcgcg gtggtgttca atcccgtgcc gggcccgccg atgggggcca
      421 gcacgcggct ctcgttccgc tacaagctgc gcgggaccga ccgcctccgc gtgcagctgt
      481 tcagcctttc gaacggctat caccgctatc tcagtctcgc cggcctgccg caggacgcgt
      541 ggcggtctat ggcggtcgat atgacgcaga tgcgccggcc cgacggcacc ggcgggccgc
      601 tggcagccga cgagcggatc gacgacatcc agttctacat cgacccgcga gccgaattgc
      661 tcatcgacga tgtgattctc tacgacgcgt ccgcagccga tgaaaagcgg ccgttcccgc
      721 agcgaatcct gtttaccggc tggttcgaca ccggcaaaca gggccaggaa tggccgggcg
      781 aattcgagat cgtgccgcac gaaaagccgc ggacctggaa gcatgcccgc tcgattgccg
      841 gtgaaggcgg gcagcagcgg ctggtcgtcg atctccgcgg gccgcggcga ttgtcgccgg
      901 ttacggcgct gacgttccat tatcgccttc gtggcgcaga cagattgcgg atcgagctgg
      961 ccgatcgccg cggcggcacg tcgatcgccg gcactgccgc cgatttgaag cgcgacgctt
     1021 ggagcgaaac cacggttccg ttcgaagtgc ccgctgctgc tgacaaggag gcgttcgcga
     1081 ccgaaattcg cttcctgcct caggccggtg cggtcctgga aatcgacaac ctgctgcttt
     1141 atcagccggc aggcgagtag ttcgcctttc tcgaatcggc tagatggagc acaatcgagg
     1201 aaactgaatc ggggcaggca gagaggactc gcgggatggc agagcggagc gtcggccgcg
     1261 tattcggaat cgcggcttcc atggtgatgt tgctcgctgg gtgctggggg ccgcagtcgc
     1321 cgcctcccgc gccgcccgca gtcgccagcg gcggacagca atcggcggcc gagacgttgc
     1381 cctccaagcc gcccgccgaa tggaccgcgg gcgaaatcct ccagcgactg ctggccacct
     1441 atcgcagtgc ccagacctac caggatcagg gagtcgtgcg gctggcctat cgccaggacg
     1501 gtcagcccgt cagccgcgag gagcccgccc gcgtcgcctt cgagcggccg ggcaagctgt
     1561 cgctggcggc gtaccaggcg atcgtcaaat gcgacgggca atcgctgcgg gcgcgaatcg
     1621 aagatcccga ctcgcggaac atcgatggcc aggtgctggt gcggccggcc ccgcgggagc
     1681 tgaagctgac cgacctcgcc ggcgacgcgc tgctctacga cacaatcagc agcgagctgc
     1741 gccggcagcc gatccagctg gagttgctgc tggagtcgag cggcctggtt tcggcgtttg
     1801 gcgcggacgt cgcctgccgc cggctggagg acgagacgca cgaggcgcgc ccttgcttcc
     1861 gcgtcgaggt gccctcaccc ggcggagcgt tcgttttctg ggtggatcag gcgcaatggc
     1921 tgctccggcg gctggactat ccggcggcgg cgctcttgcc cgctctggcc aacgatccgg
     1981 cggtcagcga cttgcagctg ttggccgata tgcgcggggc cacgatcggc gcgacgattc
     2041 cagccgagca gttcgtgctc gaaattcccg ccggcgcgaa gcggatgaag tccttcgtga
     2101 ttccgccgcc gccattgccc agcaatctgt tcggccggca gccgccggag ttctcgttcg
     2161 tcgatctgga ggggaaggcc gtcggggcca aagagctggc cggcaagccg gccgtactgg
     2221 tgtggtacca cgacaacccg gcctgcgagg caacgctgca gcaggtgtcg ctcgctcgcc
     2281 agcggctgaa ggacagcgct gcgctgctcg cggtcgccac cgatccgacg acgaccgaca
     2341 atgccgccgt cgcccgccgg ctggccgaat ggaaggtcga gctgccggtc gcccgcgacc
     2401 tggaggcgtt tggcgacaag gcctttcgca ttcaggtgca gccgacgatc gtcgtgctcg
     2461 acccgcaggg ccgcgtgcaa atctatcagc ccggcggcaa cccggagctt gccgaccagc
     2521 tcgtcacgat cgtgcagcgc ctgtcgcgcg gcgacgacct ggcgaccgaa ttgctcgagc
     2581 ggcgggcccg cgagcagcag cagtacgaac aactcgtcgc caaagggggc gaagagccgt
     2641 cccaggtcgt ggagctgccc gaggcggtaa ttcgccgccg cacggagccc aagcagctca
     2701 agctgcagcc gctgtggacg tgcagcgagc tgaaatcgcc cggcaatctg ctcgtcgtcg
     2761 agtcggaagg ccagccgcct cggatcttcg tgttcgaagg ctggcggacg attgccgagg
     2821 tggatgctca aggcaaaatc gccggccgcc atgcgctggc gattcccgag cgtgcggccg
     2881 tcgcgctgct ccgcacggca acggccaagg acggccagcg ctacttcgcg gtcagcgcac
     2941 cactggcccc gcaggttttt gtattcgatg gcgattggaa gctggtcctg acgcatccgc
     3001 cccccggcga aacaccgctg gccgtgctcg atctggcctt cgccgatctg ggccaggccg
     3061 acggcacgcc cgatttgctc gtggccagcg tcgccgatca gggactcgcc gccgtttcgc
     3121 ttaagggcga gcagctgtgg cggaaccgcg cctttcccaa cgtggtcagt gccgccgtca
     3181 cactgcccaa cgacgccggc tcgtggggca tcctgctcac gggcgagagt ggcgcgatcc
     3241 tccgcgtcaa cggctacggc cacgaggacc cgccggccca ggtcgccggc cggcaggtcc
     3301 tcagtctgat cgccggccga tttccggcgg ccaaacaagc ggcgctcttg gccctggccg
     3361 gcggcgagcg cggcgagcgg ctggcggtgg gtttgaccag caatctcaag gaggcttgga
     3421 actacccgct gccggcgggc gtgcacgcgc ggccgatcga gccggtcgcg cccggccgcg
     3481 tcctgcccgg gcgtgagggc gagtggtggc tggctgcccc cgacggctcg attcacgtgg
     3541 tgtcggaaga cggcgacttc ttcgactcct tttactacgg cgaagtgctc accgggctcg
     3601 ccgcggcgaa gctgaaccag cgccccgcgc tgctcatcgc caccgacgcc ggcctgtcag
     3661 cctgggaggt cgctgcgcct ttgccgcggc cggcaactcg cgagcggtga ataattcccc
     3721 gccgcctgct tgcgatgggc ctgccgaaca ggtgaaacta ggcgtctgtt ctcgcagccg
     3781 gccgttcgcc gcgcctgcgc cgcttgtggg gattcgtcca ttccacttct tatctacgcg
     3841 ggagggttgt catgagaatg tcgcagcgat gggtgatgtt gtgcagcctg gccttggtcg
     3901 tcgccctgac ggcatcggcc gcggcccagc agcggcgggg cgggtttggc ttcgggttcg
     3961 gccgcgcgcc gcagggcctg gtgtcgctgg ccgcccagga gccggtgcaa aaggacctcg
     4021 gcctgagcgc cgacgtggtg ggcaagatca gcccgctgaa cgatgaatat cgggccgcca
     4081 tccagaagga aatgcaggcg gccggcactc tcgacttcca ggggctgcgc gatctgcccg
     4141 aggctgaacg ggctgccaaa ctggcggagt acacgaagaa gaccaccgaa gcctcggcca
     4201 aggtgaacac ggaatacact cccaagctcc aggccatcgt cggggccgac agcatcaagc
     4261 ggctgaagca aatccagatc caggctcagg gttcggaagc gctggccacc gcggatgtcg
     4321 ctgccgagct caagctgacc gacgaccaga agaaaaagct ggccgacctg gcgaccgaat
     4381 acacagccaa gcagcgcgag ctgtttaacc ccggcaccga tcagcaggaa gcgcgagcca
     4441 agcgggccga actgaacaag gaacgcgaca ccaaggccga ggccttgctc actgccgagc
     4501 agaagtcgaa gtacacggcc ctgaagggtc cggcgttcga cgtctcgacg cttagcacgg
     4561 gccgcggccg gcgcgggaac aacaactagc cggtcttgca ctgagacacg tcgtcgcggc
     4621 ggcgacacac tcgccgcccg cgagcagtgg cgactcgagc atcggccccg tcggcgaaat
     4681 ccggcggggc tttttcgtgc gcatcttcga tcgctcgaaa attggcacgc gattcgcaat
     4741 caggtgccag caacataacg gccggtctat ttgaacccac gccggaagga gcctcagcca
     4801 tgcgacaatc cacgatttct cgtcgcgaat tgcgaagcag cgacgttcgc ggctcgatgc
     4861 gcgatgcccg ccgccagcgg attcgcgacg cctggtcgcc cgccgagcgc agtgctcgtg
     4921 ccgaagccgc gcagcagcgc cgcgaagatc tcgcccggct cttgttcggc gcgcccgcgc
     4981 tggaaatctg ggccgtcggc gctccaggcg acgacgacct cgcccggctc gccggctgat
     5041 tcccccacga ccaatacggg ggggcgcgaa gcaccgtgcc cccagcagaa ttcgaccttt
     5101 gccaaccgga atggagtccg ttttcacact cagtcgccgg cggctgcttg ggggccgccg
     5161 gcgatttcgt ttatcaggcg accggaagac tactcgccct tggccttctc gccgggcgac
     5221 ttgccgcgca gctcgtccag ctctttgcgc agctgcttga cttccttctc cagctgctcg
     5281 accgacttgc tctcgagttg atcgagcttc ttcatgatcg cgtccagctt gccttccacg
     5341 ccgccgccgc ctgccgtttc aaggcggtag gtgcgtagcg gggcgtattg cggctgcgca
     5401 ggaatcggag gcgcgacagc ctgcggcggc ctgggagacg cgggaggcgc cggcggcgcg
     5461 ggcggaacgg ggcgcgccgc agggaccgga gttccgggcg cggccggcgt gcggccgtat
     5521 ccatagcgaa tcgtgttcgg accggaccca cccaccgctc cgggcagttt cagcgacaag
     5581 cggtggccga gaaactgctg cacgtgcgac cgcaaatcct cgggcaggtc gcccagcttc
     5641 tcctccgtca cgtcccacgt cttgccgtcc ttctcgacgt gaatcttcgc cggctcatcg
     5701 cccttcttcg tgatgctgat cgtcacatcc ttgggcaggt cggtggaatt caggacgctg
     5761 taatactttc ccgcaaccgt gggcggtacg acggcaggcc gggcgagcat caggcccagc
     5821 ggtgcagcgc cggtcttgtc cttgagctgg ctgagagctt cctccagctt cttgatctct
     5881 gcctgcacgt cgggcgctac ggcgcggagt gagaagttgc tgcccgagcc cgagtcggct
     5941 tccgtcttgg gccgcttgat cggtgcgacc ttgatcgtct gctccttgcc gccgcggagg
     6001 atgatgatcg ccagctccct ttccttggcg gcatctaccg ccttgatcaa gtcggccgcg
     6061 cctttcaggt tggcgtcgcc ggccttgatc aggatgtcgt tcgtctgaaa tttggccttg
     6121 gcggccgggc tgtcggccat tacatcggcg acggccagtc cgtgctccag gccgagctgc
     6181 tgccggacga tcgcgggcaa ctctcccccc agcgcaatgc ctatccaata gtcggacgtc
     6241 ggcagcgcgt ccttatcctc ggcctcaccg gccccgcctt cggccaggcg aatcaggaag
     6301 tcgccatcga cctcctccac gacacggggc cgccgcactt gcacgggcac agactgtccg
     6361 ctggcccacg ggccagccca cacggccgac gcggcgacca ggccgcacgc cagccactta
     6421 ttccacgatg acttcataat ctcgacccct ttgaaaaaga tgagt
//
LOCUS       DASRJB010000538         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_147104, whole genome shotgun sequence.
ACCESSION   DASRJB010000538 DASRJB010000000
VERSION     DASRJB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147104"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1189
                     /locus_tag="VFB80_13960"
     CDS             170..1189
                     /locus_tag="VFB80_13960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN34928.1"
                     /translation="MQPNVWIVLVNYNGLEDTCACLESIAKIEYPRLTTVVVDNASQV
                     DPRVVLNERFGWCRVMMNPVNAGWAGGNNVGLKAALIAGADLVVLLNNDTVVSPQLVE
                     RVVAAAAANPEIGILGPVINEMNEPALVQTDGVLFNHERDAGFFSREAVPLDAEQPAV
                     REVDIVNGCCIAIRREVADRIGLIDERFFLIHEESDYCLRAREAGFRCGVIGQSLVWH
                     KHSASFKRSGQRIQRYYDARNLHLLLRTHVGPHRPTHGASRGGRQSWLHYYRYAYWRY
                     CLEREQGQDDAALAVLEGVADAWRGHYGPWARRQRLLVGPLRAGFEALRWWKGWATMS
                     AAEAS"
     gene            1192..2418
                     /locus_tag="VFB80_13965"
     CDS             1192..2418
                     /locus_tag="VFB80_13965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN34929.1"
                     /translation="MRFLFVKSALAWPRGSGHDVHTYHMMRALAEAGHEVSLTTAEPP
                     SEQAVAGLMLANRWTWGEAASRAENGQPLLRGLQERFRSYWGIEERDIRAIGKQAEQS
                     SADAVVVVGLEVLPLLGAVRKAQRVWYAADEWVWHHLSLVRLLDRGTWAHVPSAAVKG
                     LYEFAFRKLIDRAWVVSQADRRAMRWLAGIRHVDVAPNGVDTEHYRPHDVAADEHSCV
                     FWGRLDFEPNIQALEWFGRRVWPRVRERQPAAELRVFGFQPTAVVQRLSQQLGIRLKA
                     NLPDLRAEIAKSQVVVLPFQSGGGIKNKLLEAASMARPIIGSRRACNGLVLPAERPLV
                     IAGSERQWVSELLALWADQPRREALGKAARQWVIDRHSWSRAAAIAVAGLAQSQHPAS
                     AASNLQEPAASEALVG"
     gene            2424..>2693
                     /locus_tag="VFB80_13970"
     CDS             2424..>2693
                     /locus_tag="VFB80_13970"
                     /inference="COORDINATES: protein motif:HMM:NF039329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34930.1"
                     /translation="MSLVEIESPPSRRTAQLVVPLRIWGSPSEQAVPLSFGVPLPLGK
                     WRGGSAQLRAAGHSPATCQTKVLQHWPDGSACWVLVSGTVPAAGDG"
BASE COUNT          418 a          778 c         1041 g          456 t
ORIGIN      
        1 ttgcggcggg ccggcggcct gaccgcgggg agcgcggcgt acgaggcgct gtgctggctg
       61 cccgcgccgg tgaccggcgg gctgcggagg atcaagtggg cagtgactgg atagggttca
      121 gggttcgggt tcagggttca gcaggggaca ggcgtcaggg gacagaccga tgcagccaaa
      181 cgtttggatt gtgctggtca actacaacgg gctggaggat acctgcgcgt gcctggagtc
      241 gatcgcgaag atcgagtatc cgcggctgac gaccgtggtg gtcgataatg cgtcgcaggt
      301 cgatccgcgg gtggtgctga acgagcgttt tggctggtgc cgggtgatga tgaatccggt
      361 caacgccggc tgggcgggcg gcaacaacgt gggactgaag gcggcgctga tcgccggggc
      421 ggacctggtc gtgctgctca ataacgacac ggtcgtcagc ccgcagctcg tcgagcgggt
      481 ggtcgcggca gccgcggcga atcccgagat cggtattctg gggcccgtca ttaacgaaat
      541 gaacgagccg gccctggtgc agaccgacgg cgtgctgttt aaccacgagc gcgacgcggg
      601 gttcttcagc cgcgaggcgg tgccgctgga cgccgagcag ccggcggtgc gggaggtgga
      661 catcgtcaac ggctgctgca tcgcgattcg gcgcgaggtg gcggatcgga tcgggctgat
      721 cgacgagcgg ttctttctga ttcatgagga atcggactat tgtctgcggg ctcgggaggc
      781 gggctttcgc tgcggcgtga tcgggcagtc gctggtatgg cacaagcatt ctgcgagctt
      841 caagcggtcg gggcagcgga ttcagcggta ttacgacgcg cggaacctgc acctgctgct
      901 gcggacgcac gtcggcccgc acaggccgac gcacggggcc agccgcggcg gacggcagtc
      961 gtggctgcat tactaccggt acgcgtattg gcggtattgc ctggagcgtg agcaaggcca
     1021 ggacgacgcg gccctcgcgg ttttggaggg cgtggccgac gcttggcgcg ggcactatgg
     1081 gccgtgggcc cggcggcagc ggctcctggt ggggccgctg cgagccggtt tcgaggcact
     1141 gcggtggtgg aaagggtggg ccacgatgag cgcggccgag gcaagctaaa aatgcgattt
     1201 ctcttcgtca aatcggcgct ggcgtggccg cgcggcagcg ggcacgacgt gcatacgtat
     1261 cacatgatgc gggcgctggc cgaagcgggg cacgaggtct cgctgaccac ggccgagccg
     1321 ccgagcgagc aggcggtggc aggattgatg ctggccaacc gctggacgtg gggcgaggcg
     1381 gcaagccgtg cggaaaacgg tcagccgctc ttgcggggct tgcaggagcg gtttcgctcg
     1441 tattggggca tcgaagagcg ggacattcgg gcgatcggca agcaggcaga gcagtcttcc
     1501 gccgacgcgg tcgtcgtggt gggcctggaa gtgctgccac ttctgggcgc cgtccggaag
     1561 gcccagcgtg tctggtatgc agccgacgaa tgggtctggc atcacctatc cctcgtgcgg
     1621 ctgctggatc gcggcacgtg ggcgcacgtg ccgtccgcgg cggtgaaagg gctgtacgag
     1681 tttgcgttcc gtaagttgat cgatcgggcc tgggtcgtgt cacaggctga ccggcgagcg
     1741 atgcggtggc tggcgggcat tcgccacgtc gatgtcgcgc cgaacggggt cgataccgag
     1801 cattaccggc cgcatgacgt ggccgccgat gagcacagct gcgttttttg ggggcggctg
     1861 gatttcgagc cgaacattca agccctggag tggttcggcc ggcgcgtctg gccgcgggtc
     1921 cgcgagcgac agccggcggc ggagctgagg gtgtttggct ttcagccgac ggcggtggtc
     1981 cagcggcttt cccagcagct gggaatccgc ctgaaggcga atctgccgga tttgcgggct
     2041 gagatcgcca aaagccaggt cgtggtcctg ccgtttcaaa gcggcggcgg gattaagaac
     2101 aagctgctcg aggcggcgag catggcacgg ccgatcatcg gctcgcggcg ggcttgcaac
     2161 gggctggtgc tgccggccga gcggccgctc gtgatcgccg gcagcgagcg ccaatgggtc
     2221 agcgagttgc tggcgctgtg ggccgatcag ccgcggcgcg aagcgctggg caaggctgcg
     2281 cggcaatggg tcatcgaccg gcattcatgg tcgagggcgg cagcaatcgc cgtcgccggt
     2341 ctggcgcagt cgcagcaccc tgcgtccgcc gcctcgaact tacaggaacc ggcggcgagc
     2401 gaagctctcg tcggctgaac aacatgtcgc tcgttgagat agagtcaccg ccctcgcgcc
     2461 gcacggctca gctggtcgtg ccgctgcgca tctggggcag cccgtccgag caggccgtcc
     2521 cgctgagctt tggcgtaccg ttgccgctgg gaaaatggcg tggcggatcg gcacaactgc
     2581 gggccgcggg ccactctcct gccacgtgcc agaccaaggt cctccagcat tggccggacg
     2641 gcagcgcctg ttgggtcctg gtaagcggca cagttcccgc ggccggcgac ggc
//
LOCUS       DASRJB010000539         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3530956, whole genome shotgun sequence.
ACCESSION   DASRJB010000539 DASRJB010000000
VERSION     DASRJB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3530956"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..927)
                     /gene="folE"
                     /locus_tag="VFB80_13975"
     CDS             complement(253..927)
                     /gene="folE"
                     /locus_tag="VFB80_13975"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZN34931.1"
                     /translation="MPGPTSVDAISRDSDAGCGGNGAPSGTIVRNGTVLAAVDFPRIE
                     RAVREILAAVGEDPDREGLVETPARVARMYAEMFSGLAQDPREHTQKFFSEQYDEVVL
                     VRDISFCSMCEHHLLPFIGTAHVAYIPNGKVIGLSKIARVVEVISRRPQVQERMTEEI
                     ANLLVGELGAKGVAVVIEASHSCMTIRGVKKPGSVMVTSAMKGLFRSNLSTRAEIMQL
                     IYGSRT"
     gene            complement(1003..2403)
                     /locus_tag="VFB80_13980"
     CDS             complement(1003..2403)
                     /locus_tag="VFB80_13980"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34932.1"
                     /translation="MHRSTCLYGAVLGCLSWLLVGAAWGQDWPQWRGPNRDGKVTGFK
                     APATWPKELKEKWKTTVGDGVSTPALVGDRLYVFTREGGEEVIRCLNAADGKEIWQDK
                     YAGEPATGSAGGFPGPRSSPAVADGKVVTLGVRGVVSCLEAASGKLLWRKDDYPGLAP
                     QFFASSSPLIQDGVVFVQLGGVEAGRGFVGGGGRGTGGVIAYDLSTGAKKWEWKGDAP
                     AYGSPILATVAGEKTLLMPTAGNMAALGTADGKQLWFVSYTQGRYNAATPIVTGDTVI
                     YGGLQVGMSAEKLAKKGSELEGTIVWTNDQHSLIYNTPVLKDGKLYGLSTTSNLFCLD
                     AETGKTAWSAPLNPGAAGAAAPAPAKAGGGGGFGGGAGGGFGGGGGGGKGKGGGGRGG
                     FGNTAGYGSVVDAGSVLFALSPAGSLVVYEPNPTEYKELARYKVAEAGTYAYPIIAGN
                     RIFIKDATAVKLLAIE"
     gene            complement(2583..>2972)
                     /locus_tag="VFB80_13985"
     CDS             complement(2583..>2972)
                     /locus_tag="VFB80_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN34933.1"
                     /translation="PELVESRYDDAFLYLNTHCKKRSLIVLVSNVIDEVNAHQIESYL
                     RNLVGRHLPLGVLLRDHQLFDAADREPSGDEQLYQAAAAAEILTWRHQILRDLERHGI
                     LALDVFPEDMTAPLVNSYLEIKARHLL"
BASE COUNT          512 a          988 c          943 g          529 t
ORIGIN      
        1 tgcgcagcgg gcgtgagccc catcgccacg gccacgccga gcgtcaggcg aaacaggaac
       61 tcgacgagga tttggctgaa caagcaaaga gtccaaggtc caaagtccaa ggtccaaagt
      121 cagcaacctg ggactttgga ccttggactt tggactcgtt tcaccactca cctactcact
      181 actcaccacg cacggctcgg cgggagcctc gccctcccga atgaccactg acgactgacc
      241 actgacgacc gcctatgtcc gactcccata aatcaactgc atgatctcgg ctcgcgtcga
      301 aaggttcgag cggaagagtc ccttcatggc cgacgtgacc atcacgctcc ccggcttctt
      361 cacgccgcgg atcgtcatgc aactgtgcga ggcctcgatg accacggcca ctcctttggc
      421 ccccagttcg cccacgagca ggttggcgat ctcctcggtc atccgctcct ggacctgcgg
      481 gcggcgcgag atgacctcga cgacgcgggc gatcttcgac aggccgatca ccttgccgtt
      541 gggaatgtat gccacgtgtg ccgtgccgat gaacggcaat aggtgatgct cgcacatgct
      601 gcaaaagctg atgtcgcgga ccaacacgac ctcgtcgtac tgttcggaga aaaacttctg
      661 cgtgtgctcg cgcgggtcct gtgcgagacc gctgaacatc tcggcgtaca tccgggccac
      721 gcgggccggc gtttcgacga gcccttcgcg gtcgggatct tcgccgaccg cagcgagaat
      781 ctcgcgcacc gcccgctcga tgcgcggaaa atcgacggcc gccaggacgg taccattgcg
      841 gacgatcgtg cccgacggcg ctccattgcc gccgcaacct gcgtcgctgt cacgacttat
      901 ggcatcgact gaggttgggc cgggcatggc ggaccgctgc gtatggcgtg gcgatgactc
      961 tcctttgatc gtagtagtcc aaggggaacg cggtcaagga cttcactcaa tcgcgagcag
     1021 cttcaccgcg gtggcgtctt tgatgaagat gcgattgccg gcgatgatcg gataggcgta
     1081 cgtgccggct tccgcgactt tgtaccgggc gagctccttg tactcggtcg gattgggctc
     1141 atagacgacc agcgagccgg ccggcgagag ggcgaacaat acggagccgg catcgacgac
     1201 cgagccgtag ccggcagtgt tgccaaagcc gccgcgtccg ccgccacctt tgcccttccc
     1261 accgccgccg ccccctccaa atccgccgcc ggcgccgcca ccgaatccgc cgccaccgcc
     1321 ggctttggcc ggtgccggtg cggctgcgcc cgctgctccg ggattcagag gagcggacca
     1381 ggcggtcttg ccggtctcgg cgtcgaggca gaacaaattg ctggtcgtgg agaggccgta
     1441 gagcttgccg tccttgagca cgggcgtgtt gtagatcagc gaatgctggt cgttggtcca
     1501 aacgatggtg ccctccagct cgctgccttt cttggcgagc ttctcggccg acatgccgac
     1561 ttgcaggccg ccgtagatga ccgtgtcgcc ggtgacgatc ggcgtcgcgg cgttgtagcg
     1621 gccttgcgta tagctgacga accagagctg cttgccgtcg gccgtgccca gcgcggccat
     1681 gttgccggcg gtcggcatca gcagcgtctt ctcgccggcg accgtcgcca ggatgggcga
     1741 gccgtaggcc ggcgcgtcgc ccttccattc ccatttcttc gcgcccgtgg acaggtcgta
     1801 ggcgatgacg ccgcccgtgc cgcggccgcc gccgccgacg aagccccgac cggcctcgac
     1861 gccgcccaat tgaacgaaga ccacgccgtc ctgtatgagc ggcgagctgg acgcaaagaa
     1921 ctgcggcgcg aggcccgggt agtcgtcctt tcgccacagc agcttgccgc tggcggcttc
     1981 caggcaggat acgacgccgc gcacccccag cgtgacgacc ttgccgtcag ctacggccgg
     2041 cgagctgcgc gggccgggaa atccgcccgc cgagcccgtc gccggctccc cggcgtactt
     2101 gtcttgccag atttccttgc cgtccgccgc gttcaggcag cggatcactt cttcgccgcc
     2161 ctcgcgggtg aatacgtaca gccgatcgcc caccagggcg ggcgtggaga cgccgtcgcc
     2221 aacggtcgtc ttccacttct ctttcagctc cttcggccag gtcgctgggg ctttgaagcc
     2281 cgtgactttc ccgtcgcgat tggggccgcg ccactgcggc caatcctgcc cccaggcagc
     2341 gccaaccaac aaccaggaca ggcagccgag aaccgcccca tataggcaag tcgagcgatg
     2401 catgatgggt cctttcgtga cgcggattag actccgaagg gattgcggac gaacgccgcg
     2461 aaggtttaac cccgccgccg gcccaaggtt gcggccgccg gtttggaaat attcaccgcc
     2521 gtggatgact agcgcagaac tgcgacaaaa aatatgtgtg ttgtccgtaa gcggacgctg
     2581 gcctacagca ggtgccgcgc tttgatttca agatagctgt tcaccagggg cgcggtcatg
     2641 tcttcaggaa agacgtcgag ggcgaggatg ccgtggcgct ccaggtcgcg gaggatctgg
     2701 tgccgccagg tgagaatctc cgcggcggcg gcggcctggt agagctgctc gtcgccggac
     2761 ggctcgcggt cggcggcgtc gaacagctgg tgatcccgca gcagcacacc cagcggcagg
     2821 tgccggccga ccaggttccg caggtagctc tcgatctggt gggcgttcac ctcgtcgatc
     2881 acgttgctga ccagcacaat cagcgaacgc ttcttgcagt gcgtattgag atagaggaag
     2941 gcgtcgtcgt agcgcgactc gaccagctcg gg
//
LOCUS       DASRJB010000540         7129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4098137, whole genome shotgun sequence.
ACCESSION   DASRJB010000540 DASRJB010000000
VERSION     DASRJB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4098137"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VFB80_13990"
     CDS             complement(<1..1501)
                     /locus_tag="VFB80_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN34934.1"
                     /translation="MSQLFAKKSLKVLLADMESDNRLKRALGPVTLTSLGIGAVIGAG
                     IFAATGAAAHNTAGPALMVSYMVAGLACIFAALCYAEFASMAPVAGSAYTYAYTTMGE
                     LFAWIIGWDLVLEYAVGASTVANAWSGYFQKVLKGIGIHIPEALGKAPLDWNHDAKWG
                     DPANWITFTGSYVNLPAVIIVAIVTAILVKGISESAGFNALMVFIKVAAVLFVVLVGA
                     FYINPANWTQVTAPDGRYGFAPYGWGGFSFFGIKQLTIGQVVEGRPVGMLAGAALIFF
                     AYIGFDSVSTHAEEAKNPKRDVPIGIVASLVICTILYVAVVAVLTGMVPYYKIDINAG
                     VSSAFAERGLKWAEFIVATAGVAGITSVLLVMMLSGPRVFLAMARDGLVPKAFFADVH
                     PTFRTPWKSTIAIGIFVAVLAGLLPINALLELTNIGTLFAFVVVCAAVLVMRRINPEA
                     HRPFRCPLVPVIPVLGILLCLMLMMSLPVGNWMRLFGWMIIGLIIYFSYS"
     gene            complement(1625..2650)
                     /gene="dusB"
                     /locus_tag="VFB80_13995"
     CDS             complement(1625..2650)
                     /gene="dusB"
                     /locus_tag="VFB80_13995"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZN34935.1"
                     /translation="MLLTEPLPQVKPLFIGPLLVDPPILQAPMAGYTNYAFRQIVREY
                     GGVGLQATEMVSARGFVWMDQNEAEHPERLWGVKEEPRPLAVQMWDNDPETLAKVGER
                     LANEYQVSVVDINFGCPVKDVTEKAHSGSYLLKFPERIHAIVSKVVEVCRPTPVTAKI
                     RLGCTRDRLTCFEVAQAVEAAGAAALTVHGRVAADFFKGTADWNQIAEIKQHLARIPL
                     IGNGDLDSAEKVVEAFRRYGVDGVMIARASLGRPWLFAQAAAALRGEPIPPEPTLAEQ
                     KECLLWHYELCCRRFGDEKGTQLMRKFACNYAQGKTGARFFRTHVAHVATRADFLAVV
                     EHYFPRE"
     gene            complement(2788..3447)
                     /locus_tag="VFB80_14000"
     CDS             complement(2788..3447)
                     /locus_tag="VFB80_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN34936.1"
                     /translation="MPRTIAIGDIHGCSAALRALIAAIQPEADDTLVPLGDYVDRGPD
                     SRGAIELVLELEQQCRVVPILGNHELMLLDALANPRVIGPWSECGGDATVRSYASLAA
                     IPPEHLDFIRRCRRYYETPTHFFVHANYAADIPLDEQPDYLLFWEHLFLHTPPAHENG
                     KIAVVGHTSQKTGEIFDLGHVVCIDTFCHGGGWLTALEVQSGRVWQADRSGRLRDGAK
                     E"
     gene            3578..4687
                     /locus_tag="VFB80_14005"
     CDS             3578..4687
                     /locus_tag="VFB80_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZN34937.1"
                     /translation="MSLLDFNAEYPRGRQKRLLAEMQRLKLDLVIVQTIEHVQWLSGA
                     RYPWTMSPAAVLRADGHLLVVAPRKPILEVAAHEIVNYEAQWHSTLRNDQREASSVEL
                     LKALSGKTGFKRIGVEFSCFTSHLAALPGERIDIEPALFRLRRRKDADELARIKQAIA
                     GTEKMYALARQIIEPGITEIEVFNRLQAAAVQEFGEMLTGTGNDYQCGARGGPPRNNR
                     RAQDGELYILDLGPAFRGYFADNSRAIAVNRRPTEVQQTAWTHVMKVFQHVEKAARPG
                     KNCQELFHEAQAILNEAPVGIFNHHLGHGIGLFPHEAPHLNPHWSDTFEVGDVFTCEP
                     GLYDEQQLRAGMRLENDYVVTASGVENLCPFKMEL"
     gene            4751..6763
                     /locus_tag="VFB80_14010"
     CDS             4751..6763
                     /locus_tag="VFB80_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN34938.1"
                     /translation="MSGLNPAQQEAVNTLAGPMLVLAGAGSGKTRVVTFRIANLIKHR
                     TRPERILAVTFTNKAAAEMQERIGGLLGKLKERPKISTFHSHCVRVLRRHARKLGYPE
                     RFAIYDRGDQESLARGVLREIRVPSESLRPADLLFLISRWKTHALRPAEASKAAQSDK
                     EHLAAMGYRRYQKSLQNAGAMDFDDLLLLTEELFGKHSAARKEEAALFDHVLVDEYQD
                     TSGVQYRIIKALAAEHRNLCVVGDDDQSIYGWRGAEVRHILRFGQDWPDAKVIRLDWN
                     YRSTAAILEIANRLIHFNKVRHDKTLQAARAGGEPPRILQFPSETDEAGQTVADIRRR
                     IEGDRLQPKDFAILFRTNEQPRAFETELRKAKIPYVILGSQSFFDRKEVKDILAYLRV
                     IERPEDEVSLLRIINNPPRGIGEKTIESLLKEAVSRGRPVWEVMKVAGTLRVPSAQEA
                     VGRFGMLIEKYHRQLESPHPGPLPKGEGVLVALVRRLIAEIGYEAELKRLYSDPNEQQ
                     ARWQSVEEVVNALGIYERGTKRPTLGGFIDEVTLGQQDMADEKEKQLAKNAVALMTLH
                     SAKGLEFPHVYMVGLEEGILPHHRSLGESDDVEEERRLCYVGVTRAMERLTLSLSLTR
                     MKWGKPRDTIPSRFLYEIMGQAEKAPTSGPRRTQRAGRTPKVAAGE"
BASE COUNT         1408 a         2257 c         2302 g         1162 t
ORIGIN      
        1 ggctgtagct gaagtagatg atcaggccga tgatcatcca gccgaacagc cgcatccaat
       61 tgcccacggg cagcgacatc atcagcatca ggcacagaag gatgcccagc acggggatga
      121 cagggaccag cgggcagcgg aagggccggt gtgcctcggg gttgatccgc cgcatgacca
      181 ggaccgcggc gcagacaacg acaaaggcga acagcgtacc gatgttggtc agttccagca
      241 aggcgttgat cggcaacagc cctgccagca ccgccacaaa gatgccgatg gcgatggtgc
      301 tcttccacgg agtgcgaaac gtcggatgca cgtcggcgaa gaaggccttg gggacgagcc
      361 cgtcgcgggc catcgccagg aagacgcgcg ggccggacag catcatgacc agcagcacgc
      421 tggtaatgcc ggccacgccg gccgtggcga cgatgaattc ggcccacttc aggcctcgtt
      481 cggcgaacgc actggagacg cccgcgttga tgtcgatctt gtagtacggc accatgccgg
      541 tcagcacggc gacgacggcg acgtagagga tggtgcagat gaccagcgag gcgacgatcc
      601 cgattggaac gtctcgcttc ggattcttcg cctcctcggc gtgagtgcta acggaatcga
      661 agccgatata ggcgaaaaaa atcagggccg cgccggccag catgccgacc ggccggcctt
      721 ccacgacctg tccgatcgtt aactgtttga tgccgaagaa gctgaacccg ccccagccgt
      781 acggagcgaa gccgtagcgg ccatccggcg cggtgacctg agtccagttg gcgggattga
      841 tgtagaaggc gccgacgagg acgacaaaca gcaccgcggc gaccttgata aagaccatca
      901 gcgcgttgaa cccggccgat tcgctgatgc ctttcaccag aatcgccgtg acaatcgcga
      961 cgatgatgac cgcgggcaga ttgacatagg aaccagtaaa agtgatccag ttcgctgggt
     1021 cgccccattt tgcgtcgtgg ttccaatcga gcggcgcctt gccgagcgcc tctgggatat
     1081 ggatgccgat gcccttgagc accttctgaa aatagcccga ccaggcattg gcgaccgtgc
     1141 tcgcgcctac cgcatattcg aggacgaggt cccagcctat gatccaggcg aaaagctcgc
     1201 ccattgtcgt gtaggcatac gtataggcgc tgcctgcaac cggcgccatc gaggcaaact
     1261 ccgcgtagca cagcgcggca aagatgcacg ccagtccggc gaccatgtac gagaccatca
     1321 gcgctggtcc ggccgtattg tgtgccgccg cgcctgtcgc ggcgaagatg cccgcgccaa
     1381 tcaccgcccc gatgcccaag gacgtcagcg tgaccggtcc cagcgcccgc ttgaggcgat
     1441 tgtcgctctc catgtcggcg agcaggacct tgagcgactt cttggcgaac agttgcgaca
     1501 tccgggcctc cgggtgacag aaccagcgca gcagccagcg agggcagaca agcgtggtta
     1561 agtatcttcg cccgctatct agcgtcaagc ggaaaagggc ggccgttgtt tcggcggccg
     1621 gtgactactc ccgcgggaaa taatgctcga cgacagcgag gaagtcagcg cgggtggcga
     1681 cgtgggccac gtgcgtgcgg aagaagcggg cgccggtttt gccttgggcg tagttgcagg
     1741 cgaacttccg catcagctgc gtaccctttt cgtcgccgaa gcggcggcag cagagctcgt
     1801 agtgccagag caggcactcc ttctgctcgg cgagcgtcgg ctcgggtggg atcggctcgc
     1861 cgcgcagggc ggcggccgcc tgcgcgaaca gccacggccg gccgagcgac gcccgagcga
     1921 tcatcacgcc atccacgcca tagcggcgga acgcctcgac cactttttcg gccgaatcga
     1981 ggtcgccgtt gccgatcagc ggaatccgcg cgaggtgctg cttgatttcc gcaatctggt
     2041 tccagtcggc cgtgcccttg aaaaagtcgg cggccacgcg gccgtgtacc gtcagcgccg
     2101 ccgcgccggc cgcctcgacg gcttgggcga cctcgaagca agtcagccga tcgcgcgtgc
     2161 agcccaggcg aatcttggcc gtcaccggcg tcggccggca gacctcgacc accttcgaca
     2221 caatcgcgtg aatccgctcg gggaacttca gcaggtacga gccgctgtgg gccttttcgg
     2281 tcacgtcctt caccgggcag ccgaagttga tatcgacgac gctgacctgg tattcattgg
     2341 ccagccgctc gccgaccttc gccagagtct ccgggtcgtt gtcccacatc tgcacggcca
     2401 gcggccgcgg ctcctctttc acgccccaca gccgctcggg atgctcggcc tcgttctggt
     2461 ccatccagac gaagccgcgg gccgaaacca tctcggtcgc ctgcaatccg acgccgccgt
     2521 attcgcggac gatctgccgg aaggcgtagt tggtgtagcc cgccatcggc gcctgcaaga
     2581 tcggcggatc gaccagcagc ggcccgatga atagcggctt gacctgcggc agcggttcgg
     2641 tgaggagcat ggttttagtc tggctgtctt ggcagcgccg cgccaagccc gatggaccga
     2701 tgacggagag acagagggac ggagcgaagg agggaaatga gcgaacgcgt gtatgtcgct
     2761 ccgtcgctcc ttccctccgt ctctctgtca ctccttcgct ccgtctctca agcgtcccga
     2821 ccggtccgcc tgccaaacac ggcccgactg cacctccagc gccgtcagcc agccaccgcc
     2881 gtggcagaag gtgtcgatgc acacgacgtg gccgaggtca aagatctcgc cggtcttttg
     2941 cgaggtgtgg ccgaccaccg cgattttgcc gttctcgtgc gccggtgggg tgtgcaaaaa
     3001 gaggtgctcc caaaagagca ggtagtcggg ctgctcatcg agaggaatat cggcggcgta
     3061 attggcgtgg acgaaaaagt gcgtcggcgt ctcgtaatac cgccggcagc ggcgaatgaa
     3121 atcgagatgc tcgggtggga tggcggccag cgaagcgtag ctgcggaccg tcgcatcgcc
     3181 gccgcattcc gaccagggtc cgatcacgcg tggattggcc agggcgtcga gcagcatcag
     3241 ctcgtggttt cccaggatcg gcacgacgcg gcactgctgc tccaattcca acaccagctc
     3301 gatcgccccg cggctgtcgg gtccgcgatc gacgtaatcc ccgagcggca ccagcgtatc
     3361 gtccgcctcc ggttgaattg ccgcgatcaa ggcgcggagc gccgccgagc agccgtgaat
     3421 gtcgccgatg gcgatggtgc ggggcatgtt gggctcgtga ttcgacccgc tggcggggtg
     3481 gggcgggctg cgataggtta gccgaaactt cctaagtggg gcaggctttc cagcctgccc
     3541 cacaaacctg gcaggctgga aagcctgccc cactacgatg tccctcctcg attttaacgc
     3601 cgagtacccc cgcgggaggc agaaacgcct gctggccgaa atgcagcggc tgaagctcga
     3661 cctggtcatc gtgcagacga tcgagcacgt gcagtggctc tccggcgcgc ggtacccctg
     3721 gacgatgtcg ccggccgccg tgctgcgcgc cgacggtcat ctgctggtcg tcgcgccgcg
     3781 gaagccgatc ctggaagtgg cggcgcacga gatcgtgaac tacgaagccc agtggcacag
     3841 cacgctgcgc aacgaccagc gcgaggcgtc gagcgtggag ctgctgaagg ccttatcagg
     3901 aaagaccggc ttcaagcgga tcggcgtcga attctcctgc ttcacgtcgc acctggcggc
     3961 gctgcccgga gagcggatcg atatcgagcc ggcgctgttc cggctgcgcc gccgcaaaga
     4021 tgccgatgaa ttggcccgca tcaagcaggc gatcgccggc acggaaaaga tgtacgctct
     4081 ggcccggcag atcatcgagc cgggcatcac ggagatcgaa gtcttcaacc gcttgcaggc
     4141 cgcggccgtc caggagttcg gcgaaatgct gacggggacc ggcaacgact atcagtgcgg
     4201 ggcccgcggc ggtccgccgc ggaacaaccg ccgggcgcaa gacggcgagc tgtacattct
     4261 cgatctcggc ccggcctttc gcggctactt tgccgacaac tcgcgggcca tcgccgtcaa
     4321 ccggcggccg accgaagtgc agcagaccgc ctggacccac gtgatgaagg tcttccagca
     4381 cgtcgagaag gcggccaggc ccggcaagaa ttgccaggag ctgtttcacg aggcccaggc
     4441 gattctcaac gaagccccgg tcggcatctt caaccaccac ctcggccacg gcatcggcct
     4501 gtttccgcac gaagcgccac acctcaaccc gcattggagc gacacgttcg aagtcggcga
     4561 cgtcttcact tgcgagcccg gcctgtacga cgagcagcag ctgcgggccg gaatgcggct
     4621 ggagaacgac tacgtggtga cggccagcgg cgtcgagaat ctttgtccgt tcaagatgga
     4681 actgtaggcc gggggtcggg agtcggcaga aatgcagacg acacggactc ccgactcccg
     4741 actcccgact atgtccggcc ttaatccagc acagcaggaa gcggtgaaca cgctggccgg
     4801 gccgatgctg gtcctggccg gggcaggcag cggcaagacc agggtcgtga cgttccggat
     4861 tgcgaacctg atcaagcacc gtacgcggcc ggagcggatt ctggcggtta ctttcaccaa
     4921 caaggcggcg gcggagatgc aggagcggat cggcggactc ctgggcaagc tgaaagagcg
     4981 gccgaagatc agcacgttcc actcgcactg cgtccgcgtg ctgcggcggc acgcccggaa
     5041 gctgggctat cccgaacggt ttgcgatcta cgaccgcggc gaccaggaga gcctggcccg
     5101 tggcgtcttg cgcgaaatcc gcgtccctag cgaatcgctg cgaccggcgg acttgctgtt
     5161 cctgatcagc cgctggaaga cgcatgccct gcgcccggcg gaagcctcga aggcggctca
     5221 aagcgacaag gagcatctcg cggcgatggg ctaccgccgc tatcagaaat cgctgcaaaa
     5281 cgccggcgcg atggattttg acgacttgct tcttttgacc gaggagctgt tcggcaagca
     5341 ttcggccgcg cggaaggaag aggccgcgct cttcgatcac gtgctggtcg atgagtacca
     5401 ggacaccagc ggcgtccaat accgcatcat caaggccctc gctgctgagc accgcaacct
     5461 gtgcgtcgtc ggcgacgacg accagtcgat ctacggctgg cgcggagccg aagtgcggca
     5521 catcctgcgg ttcggccagg actggccgga tgccaaggtg atccgcctgg attggaacta
     5581 ccgttcgacg gcggcgattc tggagatcgc caaccggctg atccatttca acaaggtccg
     5641 gcacgacaaa acgctgcagg cggcccgcgc cggcggcgag ccgccgcgga ttttgcagtt
     5701 tcccagcgaa accgatgaag ccggccagac cgtcgccgac atccgccgcc gaatcgaggg
     5761 cgaccgcttg cagccgaagg actttgcgat cctgtttcgc accaacgagc agccgcgggc
     5821 gtttgagacg gagctgcgga aggcaaaaat accgtacgtg atcctgggca gccagtcgtt
     5881 cttcgaccgc aaggaggtca aggacatcct ggcgtatctg cgggtaatcg agcggcccga
     5941 ggacgaggtg tcgctcttgc ggatcatcaa caacccgccc cgcggcattg gcgagaagac
     6001 gatcgagtcg ctgctcaagg aagcggtcag ccgcgggcgg ccggtgtggg aggtgatgaa
     6061 agtagcaggc acactccgtg tgccgtcggc tcaagaggcg gttggcagat tcggaatgct
     6121 tattgagaaa tatcatcgtc agcttgaaag ccctcacccc ggccctctcc caaagggaga
     6181 gggagtgctc gtcgctctcg tccgccggct gattgccgaa atcggctacg aagcggagct
     6241 gaagcggctc tacagcgatc cgaacgagca gcaggcccgc tggcaatcgg tcgaggaggt
     6301 cgtcaacgcg ctgggcattt acgagcgcgg cacgaagcgg ccgacgctcg gcggcttcat
     6361 cgacgaggtc acgctcggcc agcaagacat ggccgacgag aaggaaaaac agctcgccaa
     6421 gaacgccgtg gccctgatga cgctgcactc ggccaagggc ctggagtttc cgcacgtcta
     6481 tatggtcggc ctggaagagg gcatcctccc gcaccaccgc agcctcggcg agagcgacga
     6541 cgtcgaggaa gagcgccgcc tctgctacgt cggcgtcact cgcgcgatgg agcgcctgac
     6601 cctctcgctc tcccttacgc gcatgaaatg gggcaagccg cgggacacga tccccagccg
     6661 gtttctgtat gaaatcatgg ggcaggccga aaaggcgcca accagcggcc cgcgccggac
     6721 gcagcgcgcg gggcgaaccc caaaagtagc cgcgggggag tgaacagcgc tattcgggca
     6781 cgatgtcact gtcgcccagc tgatcagcca cttggggcgg actcgtttgc gaaaggccga
     6841 tcgccgcgga cttaatccgc ggagaagatt cggcaaccaa gccagcccca aaaccagagc
     6901 caaagtcccg catctcgaca gttcctcacc tgccccgcga cgcgagcgga atttggtttt
     6961 ttctccggat gcttagattc aagcgaatcg acatatatca agatctctaa tcccagactg
     7021 catgcctgag cgggcgaaag gtccaagctt ctcaaatgtt gcgaaaagcg actcaccgaa
     7081 acgcctgaag attgcaagtt ttccatgtag taatcggttc cccagccca
//
LOCUS       DASRJB010000541        15047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1838705, whole genome shotgun sequence.
ACCESSION   DASRJB010000541 DASRJB010000000
VERSION     DASRJB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838705"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFB80_14015"
     CDS             <1..822
                     /locus_tag="VFB80_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34939.1"
                     /translation="YWGSGFLPTKYQGVKFRGAGEPVLYLSNPPGISREVRRGLLDDL
                     RTLNEQKLAAVGDPEIATRIAQYEMAYQMQSSVPELTDLASEPQHVLDLYGPDVRRPG
                     SFAYNCLLARRLVERGVRFVQCFHAGWDHHRNLATQFEIQCRDTDQPSAALVQDLKQR
                     GLLDETLVIWGGEFGRTPFCQGDINDKKTLGRDHHPYCFTLWMAGGGVKRGFSFGASD
                     DFAHNVAENPVHVHDFQATILHLLGIDHERLTFKFQGRHFRLTDVHGRIVREVLA"
     gene            complement(954..1814)
                     /locus_tag="VFB80_14020"
     CDS             complement(954..1814)
                     /locus_tag="VFB80_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34940.1"
                     /translation="MLEREEYIEQAHLFRTLAERIADNVPMQDLLAYARQEILSTTRL
                     PLAIDFLLSELRHTGMMNSAMARLPHYFTPFQTYVMREAEAERGRFDLRIAIHILRHE
                     AEYRAKSPAPQGLFLFHFECLCRNRLKYDPGLAAVAADPLYDADWRQWIDELRTRIGM
                     VDFADMIYVRSQFNLVHRTRPNEPPPVAEKPVLFGEKEGQIAWANRRKDPLYLFAALQ
                     RHLGYPVVPRPKRPDESVNLLPLLNLRVERLEARLKLLEEEGKGGIDLRKLYGQSDRP
                     LPNVPPPDDL"
     gene            complement(1835..2371)
                     /locus_tag="VFB80_14025"
     CDS             complement(1835..2371)
                     /locus_tag="VFB80_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34941.1"
                     /translation="MKLTTRVYELEPNWLLICFKGHKPPSENRAFWFQRTLRDYLGDH
                     PDRHVRRIETIQDNGELLGLHVWLEVQPQPGKRKELPVKIHHQLFCEMQPEHMEALLH
                     YAYDIFFRHEAGSGVLAVVSRGGKVVLFDRGKEKCFVVPLAELRNLPAEALAKLRQWQ
                     SEPTTPYFTIELGGFELS"
     gene            2491..3786
                     /locus_tag="VFB80_14030"
     CDS             2491..3786
                     /locus_tag="VFB80_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34942.1"
                     /translation="MRRLLTGCLLGLFVAMASPAAAENWPGWRGPRGDGTSLEANVPV
                     KWNATMGENIAWKVAVPGIAHASPIVWQDRVILVTCLPDSQERLLLCLDRKSGKELWR
                     QAVVRAPLETKHALNSFASSTPATDGDSIYVSFLEVDGHTIPAPNVSNVRPVTPGKMV
                     VAAYDFSGKQRWLVRAGDFISAHGYCASPVLFEDLVILNGDHDGDGYIVALKKSTGEK
                     VWSSPREHKTRSYVTPIIRDVAGRTQLVFSGSKSVVSLNPRDGSTHWSIQGPTEQYVA
                     SMVFDGKLFFMAAGFPDYHVLGIRPDGSGNVTDTHIAWRETSAKCYVPSPVVAGGYLI
                     VADDRGTANCFVAATGERLWQERLGNHYSGSLVTAGGLVYLTADDGTTKVVRPGPKLD
                     VVAENPLGEFCYSSPAISQGQIFIRGEKQLVCVGGVSGE"
     gene            complement(4246..5727)
                     /locus_tag="VFB80_14035"
     CDS             complement(4246..5727)
                     /locus_tag="VFB80_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZN34943.1"
                     /translation="MPAHRFALSLVLSLGGLVLLSPAYGQNALAPPAAAAVAEDDHAH
                     AGQASIGRTLPIWTVIPFVVLLLCIAILPLAAGHWWEHNNNKLLVSGLLGALVAGYVL
                     IAFGQRGAAALEHVLTEYVSFLLLLGALYLISGGVYVKGSLSGTPLANTSLLALGAVL
                     ASFIGTTGASVLLVRPLLRANQSRVTKAHIVVFFIFIVSNCGGLLTPLGDPPLFMGFT
                     AGVPFEWTLQHLWPQWLAVNLALLVIFHLWDEVIFNMEEKARPGSQLEEVLKHEPLGI
                     RGGLNFLFLAGVVAAVYFKGVYNWPWGVQEAILAALAALAYLTTAAENRRANKFSFGP
                     ILEVAYLFVGIFVTMTPALQILNAWGGGEREVLGMQFTMTRPWQFFWASGSLSSFLDN
                     APTYITMAATAAGLHHVPARGQYLAEFLAKGPETAALLAAISCGAVFMGANTYIGNGP
                     NFMVKAIAEENGVQMPSFFGYMAYSCGILIPIFVVVTFVFFSS"
     gene            5842..6474
                     /locus_tag="VFB80_14040"
     CDS             5842..6474
                     /locus_tag="VFB80_14040"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZN34944.1"
                     /translation="MKVAILGTGAVGAALGARWAKAGHGVTFGSRQPQSEKVLQIVEK
                     CGSAAKACSPAQAVANAEAILLAVPWSVARQTLSELGDLRGRPVIDCTNPLLPDLSGI
                     ELGHSTSAAEQIAAAAPSARVVKAFNTASVKVMNDPQFADHRAAMFYCGDDAAAKAVV
                     QKLIVDIGFEPVDAGPLASARYLEPLAMLYIHLAFKQGWGSNCAFKVMKR"
     gene            complement(6557..7534)
                     /locus_tag="VFB80_14045"
     CDS             complement(6557..7534)
                     /locus_tag="VFB80_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN34945.1"
                     /translation="MFVQIFNSPMVTQLAVFGLFAALAWLAFDWFTGSGSRMEQRLED
                     FKDPLSRKRRDELGPGKKKQDAMSRMLEASAPVLSKPLRPKTEKEQSKLKLKLSHAGF
                     RSEAAPQIFLGMKFIMLVVGVLLGGGTTLAIMGLSTGSLFRVVMIAGGMFYFPDLVVF
                     YLGKKRKEKIFLGLPDALDLMVVCVEAGLGLDQALRKVAEEMKKSYRTISEEFNLCNL
                     HLQMGRAKHQVLQELGIRSGVDDLRALASILIQADKFGSSIAQALRVQSDSMRTRRKQ
                     LAEERAAKTAVKLIFPLVLFIFPGIFVVLVGPAAIVMVREMFPAMNGGG"
     gene            complement(7589..8560)
                     /locus_tag="VFB80_14050"
     CDS             complement(7589..8560)
                     /locus_tag="VFB80_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN34946.1"
                     /translation="MVVTILIVGAAFIGVACLVGGVATLVLRSSGDAEIENRLDVLTG
                     ATSPTAGKGKANEPALLASPLNDVPNFMEVFVKRFFNLRAFLQQADTQMSPLKFIVIS
                     LAMGFGTAVLLPILRAPVAVAPLGLMVGSIPFVLLFFKRKRRLAAFGKQLPEALELIG
                     RALRAGHSLASGFKLVADEMGAPIATEFERCYEAQNLGVPLEDAIEEMTNRVPNLDLR
                     FFATAVILQRQTGGDLAEILDKIGRLVRERFVIWGQIQSLTGEGRLSGIVLLALPPCL
                     FVVMWYLNPGYSMTLFTDPMGHQMLAGAIVLQVLGALIIRKIVNIKV"
     gene            complement(8586..9908)
                     /locus_tag="VFB80_14055"
     CDS             complement(8586..9908)
                     /locus_tag="VFB80_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZN34947.1"
                     /translation="MKPLATTARTADAAKAEEFDQLKRKIHNKLVDKLDLNRVGELQG
                     DVLRREIRLVVEHLCDTEETFLNRAERERLIEEVLDETFGLGPLEMLLKDHTISDILI
                     NGPKNIYVERRGKLEKTSVEFRDNAHLLQIIDRIVSKVGRRVDEVCPMVDARLTDGSR
                     VNAIIPPLALDGAAVSIRRFGSNPLKLEDLLNFKALTPEMVMLLEGAIKARLNMIIAG
                     GTGSGKTTLLNTLSSFIPNTDRIVTIEDAAELQLQQDHIVRLETRPPNIEGKGAITAT
                     DLVKNALRMRPERIIIGECRGPETLDMLQAMNTGHDGSLTTLHANTPRDAIARLETMI
                     MMSGFELPIKAMRQQIASAVHLLIQANRLQGGIRRVTNITEVCGMEQDTVVMQDIYKY
                     VQEGIDETGRARGHFEATGIRPTFMSKLESAGVRLPSLAFKQRTMLVD"
     gene            complement(10115..11401)
                     /locus_tag="VFB80_14060"
     CDS             complement(10115..11401)
                     /locus_tag="VFB80_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34948.1"
                     /translation="MTNNTRRQFLEKSSLAVAGSLATLALAPRVHAAGSDVLKVGLIG
                     CGGRGSGAASQALKADSGARLWAMGDAFEDRIDLSLQSLQKIEEVAPKLDVAKERRFV
                     GFDAYKEVIACCDVVLLTTPPQFRPLHLAAAVAAGKHIFAEKPVAVDAPGVRRVLDIC
                     EQAKAKNLSVVSGLCLRYDWRFQEIVKRIHDGQIGKPLALQANDYRGGIWVKPRQPDW
                     SDMTYQMRNWYYFTWLSGDFNVEQHVHFLDVCAWIMKNQYPTKAVGLGGRQVRTGKDY
                     GNIYDHFSVTYEYDDGTKVFSNCRQIPNCVGDMSAHVLGTKGTARINERKRGMLLKTD
                     AGDWAYDGPENNLYQTEHDELFAAIRNGKPLNNGEYMAKSTLLAVMGRMAAYTGEAIT
                     WDMAFNSKEDLTPPAYDWNIKLPEPPVAIPGVTKFV"
     gene            complement(11479..12306)
                     /locus_tag="VFB80_14065"
     CDS             complement(11479..12306)
                     /locus_tag="VFB80_14065"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN34949.1"
                     /translation="MAAARWVTLTAILAACGQVAWAQEGTQAIAPRQAIQLFNGRDLS
                     GLTTWLKESQREDPSRVFRVTEGMIHLTGGGNGYVATAKEYRDYRLVVEYKWGQRTDG
                     GKYVRNSGILLHATGPDGGAGGAWPSCIECQLAQGCVGDIIVIRGKDEQGEPIPVRVT
                     AETELAPDGRRRRWKEGGEALTFPPTRGQLWWSRHDWDFKELLDTRGKEDVESPLGDW
                     TRVECIADGSKLTIKVNGQTVNECREVFPAAGRILLQTEGHEIYFRKFEIQPVSFNP"
     gene            12419..14107
                     /locus_tag="VFB80_14070"
     CDS             12419..14107
                     /locus_tag="VFB80_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HZN34950.1"
                     /translation="MSFKAAILRGALTMWMVVGAATSVSSQDVGTQAQEFVREHERKI
                     QPLQIASARAWWTANITGQDEDFAAKEEAENKINEALSDKQQFNRLKQIQAGKIADPV
                     LRRTIDVLYLQYLDKQVDPALLRRMTAKANAIEKAFNVFRAQVKGKSLTDGEVRQVLQ
                     KSKDSQERQAVWEASKRVGAEVENELKDLVRLRNEAATGLGFPDYHALQLHLTELTQA
                     QVLKIFDELDELTRQPFDRAKSRIDEKLAASYALKPQQLMPWHYHDPFFQEAPAVYEA
                     DLDQPFADADIQRICREFYAGIGLPIDDVLAKSDLYEKRGKSPHAFCTDIDRAGDVRV
                     LGNIRPTEYWMSTMLHELGHSVYSSKNIPAELPWALRSDAHTLCTEGLAMMFERFSKS
                     AEWLRAYGVEVTEPEKFSASAAEMRRDRLLIFSRWAQVMFRFEKELYRDPSQDLNKLW
                     WDLVEKYQGVRRPPERNAPDYASKIHVVSAPAYYHNYLLGQLFACQLHASIAKEVLNR
                     SDAADAIYTREARVGKYLREKLFSQGALRSWNDVTRYATGSELNAKAFAAELQD"
     gene            14104..14997
                     /locus_tag="VFB80_14075"
     CDS             14104..14997
                     /locus_tag="VFB80_14075"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN34951.1"
                     /translation="MRPTAVNVNMDLQTLLGQTSLSRFVLDYYQRLPFSAGGAFQAAA
                     LADWGTLTALLGESGADVFICRRNERYAGDLPRDAAAATRLVQEGYTLLVRHAERHDA
                     RLARLAAAFERDFASVVNVHLYCTPAGEFGFGWHYDAEEVFIVQTVGRKQYALRKNTV
                     NPWPLEETLPADMQFEREIMPLWRCDLQPGSWLYIPSGYWHRAEARELSISLAIGLAP
                     HSALDVWDFLRRELLQSLEWRQRLPVLGRASPLTQDELRARYAQVFQALGSDLSHRLT
                     DPGAVDKYLRSRGGERTAAEL"
BASE COUNT         2653 a         4780 c         4919 g         2695 t
ORIGIN      
        1 tattggggca gcggcttttt gcccacgaaa tatcagggcg tgaaattccg cggcgcgggc
       61 gagccggtac tctatctttc gaatccgccg gggatcagcc gcgaggttcg ccgcgggctg
      121 ctcgacgacc tgcgaacgct caacgagcag aagctggcgg ccgtaggcga tccggagatc
      181 gccactcgca tcgcgcagta cgagatggct taccagatgc agtccagcgt gccggagctg
      241 accgacctgg ccagcgagcc gcagcacgtc ctcgacctgt acggcccgga cgtgcggcgg
      301 cccggctcgt tcgcctacaa ctgcctcctg gcccgccgcc tggtcgagcg cggcgtgcgg
      361 ttcgtgcagt gctttcacgc cggctgggac caccaccgca acctggcgac gcagttcgag
      421 attcagtgcc gcgacaccga tcagccttcg gccgcgctgg ttcaggacct caagcagcgc
      481 ggcctgttgg acgagacgct cgtcatctgg ggcggcgagt tcggccgcac gccgttctgc
      541 cagggagaca tcaatgacaa gaagacgctc ggccgcgacc accatcccta ctgctttacg
      601 ctctggatgg ccggcggcgg cgtgaagcgc ggcttcagct tcggcgcctc cgacgacttt
      661 gcccacaacg tcgccgagaa ccccgtccac gtccacgact tccaggccac catcctgcac
      721 ctgttgggca tcgaccacga gcggctgacc ttcaaattcc agggccgcca cttccgcctc
      781 accgacgtgc atggccggat cgtccgcgaa gtcctagcct agagccgcaa cgagctcggc
      841 ctacgagtag gccccttctg ccggaagggg cctttcggga agcgcagcga ccaggggtgt
      901 tcccgagaac tcgaccgcac cgcgctgccc aggtcccgcc cggcaggcgg gacctacaaa
      961 tcgtcgggcg gcggcacgtt gggcagcggg cggtcgctct ggccgtagag cttgcggagg
     1021 tcgatgccac ccttgccttc ttcctcgaga agcttcaggc gggcttccag ccgctcgacg
     1081 cggaggttga ggagcggcag caggttcacc gattcgtcgg gccgtttggg ccgcgggacg
     1141 accggatagc ccaggtgccg ctggagcgcg gcgaaaaggt aaagcggatc cttgcggcgg
     1201 ttggcccagg cgatctggcc ctctttctcg ccgaacagca ccggcttttc cgcgaccggc
     1261 ggcggctcgt taggccgcgt gcggtgcacg aggttgaact ggctgcggac gtagatcatg
     1321 tcggcgaaat cgaccatccc gatccgcgtc cgcagctcgt cgatccactg ccgccagtcg
     1381 gcgtcgtaca gcgggtccgc ggcgaccgcg gccagccccg gatcgtactt cagccggttg
     1441 cggcagaggc actcgaagtg aaacaggaac agcccctgcg gcgccggcga tttcgcgcgg
     1501 tattcggcct cgtgccgcag gatgtgaatc gcgatccgca ggtcgaaacg gccgcgctcc
     1561 gcctcggcct cgcgcatgac gtaggtctga aacggcgtga aatagtgggg caggcgggcc
     1621 atcgccgaat tcatcatgcc cgtgtgccgc agctcgctca agagaaagtc gatcgccaag
     1681 ggcagccgcg tcgtcgagag gatttcctgc ctggcgtagg ccagcaggtc ctgcatcggg
     1741 acgttgtcgg cgatccgctc ggcgagcgtg cggaagagat gggcctgttc gatgtattct
     1801 tcgcgttcca gcacggcagg tgagtcagca ggcgttacga gagttcaaag ccgcccagtt
     1861 cgatcgtgaa gtacggggtt gtcggttccg actgccactg gcggagtttg gccagcgctt
     1921 cggccggcag gtttcgcagc tcggccagcg gcacgacaaa gcacttctcc ttgccgcggt
     1981 cgaagaggac gaccttgccg ccgcggctga cgaccgccag cacgccgctg cccgcctcgt
     2041 ggcggaagaa gatgtcgtaa gcgtaatgca gcagggcctc catgtgctcc ggctgcatct
     2101 cgcagaacaa ttgatggtga atcttgaccg gcagctcttt gcgcttgccg ggctgcggct
     2161 gcacttccag ccagacatgc aggcccagca attcgccgtt gtcttgaatc gtctcgattc
     2221 gccgcacgtg acgatcgggg tggtcgccga gataatcgcg gagcgtgcgc tggaaccaga
     2281 aggcccgatt ctcgcttggc ggcttatgcc ccttgaagca gatcagcagc caattcggct
     2341 ccaattcgta aacgcgcgtc gtgagtttca tcaagcttga tcttagccgc ccggccgcgg
     2401 gcggtgccac ttcggttgag catccacgcc ggttgatcta tgttagagcc tcgggttttg
     2461 cctgatggaa ctcgtgaacg aggattagcg atgcgacggt tgttgactgg atgtttgctc
     2521 ggtctgttcg tcgcgatggc gtcacccgcc gcggccgaga actggccagg ctggcgcggg
     2581 ccgcgcggcg acgggacgag tctcgaggcg aacgttccgg tgaagtggaa cgcgaccatg
     2641 ggcgagaaca tcgcgtggaa ggtcgccgtg ccgggcatcg ctcacgcgtc gccgatcgtg
     2701 tggcaggacc gggtaattct cgtcacctgc ttgcccgact cgcaggaacg actgctgctg
     2761 tgcctggacc gcaagagcgg gaaggagctt tggcggcagg cggtggtccg cgcgcccctg
     2821 gagacgaagc acgcgctgaa cagctttgcc tccagcacgc cggcgaccga cggcgactcg
     2881 atttacgttt cgttcctgga agtcgatggg cacacgattc ccgcgcccaa cgtgagcaac
     2941 gtccggccgg tgacgcccgg gaagatggtc gtggccgcct acgacttcag cggcaagcag
     3001 cggtggctgg tgcgggcggg cgatttcatc agcgcccacg gctattgcgc cagcccggtg
     3061 ctgttcgaag acctggtgat cctgaacggc gaccacgacg gcgatgggta catcgtggcc
     3121 ctcaagaaat cgaccggcga aaaggtctgg agcagtcccc gcgaacacaa gacgcggagc
     3181 tacgttacgc cgatcatccg cgatgtcgcc ggccgcacgc agctcgtctt ttcgggcagc
     3241 aagagcgtcg tcagcctcaa tccccgcgac ggctcgactc actggtcgat ccaggggcca
     3301 accgagcagt acgtggcctc gatggtgttc gacggcaagc tgttcttcat ggccgccggc
     3361 tttcccgatt atcacgtgct gggcatccgc cccgacggca gcggcaacgt gaccgacacg
     3421 cacatcgcct ggcgcgagac cagcgccaag tgctacgtgc cgtcgccggt cgtggcgggc
     3481 ggctatctga tcgtcgccga cgaccgcggc accgcgaact gcttcgtggc ggctaccggc
     3541 gagcgcttgt ggcaggagcg gctgggcaac cactacagcg gctcgctcgt gacagccggc
     3601 gggctggtgt atctcaccgc cgacgacggt acgacgaagg ttgtgcgtcc cggaccgaag
     3661 ctcgacgtcg tggccgaaaa cccgctgggc gaattctgct attcttcgcc cgccatctcg
     3721 cagggccaga tttttatccg cggcgagaag cagctggtgt gcgtgggagg agtgagtggt
     3781 gagtagtcag gggtgattgc gtcaacatca attccaccac tcaccaccga ccactcacca
     3841 ctcacttttc acggccaaaa tcagccttga cccattttgg acgttggcct atcttcctgc
     3901 ccacgcttgg cggccatgcc gccgttcaag tgcgataaac agagcagccg cgaggttggg
     3961 ctcgcacctg gaacgtgtca cgagtgcgtt ccgcgggccg ctggaggcaa gtgctggcaa
     4021 ggaggccggc gcggcttttg gcttcgcctc ttcggctgcg tacggcccgg cgtcacaagg
     4081 acgtggcgct gaccgtccaa tcgctcccgg aatccgtagt gtccatcgcc ccccttggac
     4141 gctgcttgtt tcgggggctt ttttgttgcg ccctgcgggc aacaaatccc aagaaccaaa
     4201 gaaccaaagc agattggttc ttcggttctt cggttcttgg attttctacg aactgaaaaa
     4261 cacaaaggtc acgacgacga aaatcggaat caagattccg cagctatacg ccatgtagcc
     4321 gaagaagctg ggcatctgga cgccgttttc ttcggcgatg gctttgacca tgaagttcgg
     4381 tccgttgccg atgtaggtgt tggcgcccat gaagaccgcg ccgcaggaga tggcggccaa
     4441 tagcgcggca gtctctggcc ctttggccag gaattcagcc agatactggc cccgggcggg
     4501 gacgtggtgc aagccggctg ccgtcgcggc catcgtgata taagtcggcg cgttgtcgag
     4561 aaagctcgac agcgagccgc tggcccagaa gaactgccag ggccgcgtca tagtgaactg
     4621 catgccgagc acttcgcgct ccccgccccc ccaggcgttg agaatctgca gggcgggagt
     4681 catcgtgacg aaaatgccga cgaacagata cgcgacttcc aggatgggac cgaacgaaaa
     4741 cttgtttgct ctccggtttt ccgccgccgt cgtcagatag gccagggccg ccagcgcggc
     4801 caggattgct tcctgcacgc cccagggcca gttgtaaacg cccttgaaat agaccgccgc
     4861 cacgacgccg gccaggaaca ggaagttcag gccgccgcgg atgcccagcg gctcgtgctt
     4921 caggacttcc tcgagctgcg agccgggccg ggccttttcc tccatgttga agatgacttc
     4981 gtcccagagg tgaaagatca cgaggagggc caggttgact gccagccact gcggccaaag
     5041 atgctgcagc gtccactcga atggcacgcc ggcggtgaag cccatgaaca ggggcggatc
     5101 gcccagtggc gtgagcagcc cgccgcagtt ggagacgatg aaaatgaaaa agacaacgat
     5161 gtgggccttg gtgacgcggg attgattggc ccggagcaag ggccgcacga gcagcacgga
     5221 ggccccggtc gtgccgatga agctggccag caccgcgccg agcgccagca ggctggtatt
     5281 tgccagcggc gtcccgctga gcgagccttt gacgtagacg ccgccgctga tcaggtacag
     5341 cgcgccgagt agcagcagga aggagacgta ctccgtcagc acgtgctcca gggcggcggc
     5401 gccgcgctgg ccaaacgcga tcagcacgta cccggcgacc agggccccca gcaggccgga
     5461 gaccagcagc ttgttgttgt tgtgctccca ccagtgccca gcggccagcg gcaggatggc
     5521 gatgcacagc agcagcacga cgaacggaat caccgtccag atcggcagcg tcctgccgat
     5581 cgatgcctgg ccggcgtggg cgtgatcgtc ttcggccacc gcggccgcgg ccggcggcgc
     5641 gagggcattc tggccgtaag ccggtgaaag gaggacgagg ccgccaagcg aaagcacaag
     5701 gctgagggcg aaacgatggg cgggcaccga actgactcct gactgatctg ggtgaatgga
     5761 cctttcgctc cgcctcacga aatgctccaa aaaggttacg atgaagccgc cctgcccaac
     5821 aggggactcg gaggtgcatc cttgaaggtc gcgattctag gcaccggcgc ggtcggcgcg
     5881 gccctcgggg cgcgctgggc gaaggccggc cacggcgtga cgttcggctc acggcagccg
     5941 cagagcgaga aagtgctgca aatcgtggag aaatgcggct ctgcggcgaa ggcctgctcg
     6001 ccggcccagg cagtcgccaa tgcagaagcg atcctgctgg cggttccgtg gtccgtggcg
     6061 cggcagactc tttccgagtt aggcgacctg cgcggccggc cggtcatcga ttgcacgaac
     6121 ccgctgctgc cggacctgtc gggcatcgag ctggggcact ccacgtccgc tgccgagcag
     6181 atcgccgcgg ctgcaccttc ggcccgcgtc gtcaaggcgt tcaacacggc cagcgtgaaa
     6241 gtgatgaacg atccgcagtt cgccgaccac cgggcggcga tgttctattg cggcgacgac
     6301 gccgcggcca aggccgtcgt ccagaagctg atcgtcgaca tcggcttcga gcccgtcgat
     6361 gccggcccgc tggccagcgc ccgctacctg gagccgctgg cgatgctcta catccacctc
     6421 gcattcaagc aaggctgggg gagcaactgc gcgttcaagg tgatgaagcg ctgagcaggg
     6481 ctagggctgg gggttagggc tagagacgat tcaactctac accccagcct tagtccctag
     6541 gccctagccc tattcattac ccgccgccgt tcatcgccgg gaacatctcg cggaccatca
     6601 cgatcgccgc ggggccgacg agcacgacga agatgccggg gaagatgaag agcaccagcg
     6661 ggaagatgag cttcaccgcg gtcttggcgg ccctctcttc ggcgagttgc ttgcggcggg
     6721 tccgcatcga gtcgctctgc acgcggaggg cctgggcgat cgacgagccg aacttgtcgg
     6781 cctgaatcag gatcgaggcc agtgcgcgga ggtcatcgac gccgctgcgg atgcccagct
     6841 cctggagcac ctggtgcttg gctcgaccca tttgcaggtg caggttgcac aggttgaact
     6901 cctcggagat cgtgcggtag gactttttca tttcttcggc gactttgcgg agcgcctggt
     6961 ccaggcccag gccggcttcg acgcaaacca ccatcaggtc cagcgcgtcg ggcaggccca
     7021 ggaagatctt ctctttccgt tttttcccca ggtagaacac caccaggtcg gggaaataga
     7081 acatgccacc ggcgatcatc acgacgcgga acagcgagcc ggtcgaaagg cccatgatgg
     7141 ccagcgtggt gccgccgccc aagaggacgc cgacgaccag catgatgaac ttcatcccca
     7201 ggaagatctg cggggcagcc tcgctacgga agccagcgtg cgagagcttc agcttgagct
     7261 tgctctgctc tttctcggtc ttcggcctga ggggcttgga aagcacgggg gccgaggcct
     7321 cgagcatgcg ggacatggcg tcctgcttct tcttgcccgg gcccagctcg tcgcgccgct
     7381 tgcgggacag cgggtccttg aagtcctcca gccgctgttc catgcgcgag ccgctgccgg
     7441 tgaaccagtc gaacgccagc caggccagcg ccgcgaacag gccgaacacg gccagttggg
     7501 taaccatcgg gctattaaag atttggacga acatggcaga ctcgagtacg aggaagtctc
     7561 agttctttga ttctttggac tttattccct agaccttgat attcacgatc ttacgaatga
     7621 tcagggcgcc gaggacctgc aatacgatcg ccccggcgag catctgatgg cccatcggat
     7681 cggtgaacag cgtcatcgaa tagccggggt tgaggtacca catcacaacg aacaggcacg
     7741 gcggcaaggc caggagcacg atgcccgaca ggcggccttc gccggtcagc gactggatct
     7801 ggccccagat gacgaaccgc tcgcggacga ggcggccgat cttgtcgagg atttccgcca
     7861 ggtcgccgcc cgtctgccgc tgcaggatga ccgctgtggc gaagaaccgc aggtcgaggt
     7921 tcggcacgcg gttggtcatt tcctcgatgg cgtcttccag cggcacgccc aggttctgag
     7981 cctcatagca gcgctcgaac tcggtggcga tcggtgcgcc catttcgtcg gccaccagct
     8041 tgaatccgga ggccaaactg tgaccggcgc gaagggcccg gccgatcaat tccagcgctt
     8101 cgggcaactg cttgccgaac gccgccagac gccgcttgcg cttgaagaac agcagcacga
     8161 agggaatgct gcccaccatc aggcccagcg gcgcaacggc caccggcgcc cgcaggatcg
     8221 gcagcagcac cgccgtgccg aaacccatgg ccagcgagat cacgatgaac ttcagcggcg
     8281 acatctgggt gtcggcctgc tggaggaagg cgcggaggtt gaagaaccgc ttgacgaaca
     8341 cctccatgaa gttcggcacg tcgttcaggg gcgacgcgag cagcgccggc tcgttggctt
     8401 tgcctttgcc cgccgtgggc gaggtcgcgc cggtgagcac gtccagacgg ttctcgatct
     8461 cggcgtcgcc gctgctgcgg aggacgaggg tcgcgacgcc gccgacgaga caggccacgc
     8521 cgatgaacgc cgcgccgacg atcaggatgg tgacgaccat aacgaacgat ggggctagag
     8581 tggggttagt ccacgagcat ggtccgctgc ttgaaggcca gcgacggcag ccgcacgccg
     8641 gccgattcga gcttgctcat gaacgtgggg cggatgccgg tggcctcgaa atggccgcgg
     8701 gcccgtcccg tctcatcgat gccttcttgc acgtacttgt aaatgtcctg catcaccacc
     8761 gtgtcctgct ccatgccgca gacctcggtg atgttggtga cgcggcggat gccgccctgc
     8821 aggcggttgg cctgaatgag caggtgcacg gcgctggcga tctgctgccg catggctttg
     8881 atcggcagct cgaagccgga catcatgatc atcgtctcga ggcgggcgat cgcgtcgcgg
     8941 ggcgtattgg cgtgcagcgt cgtcagcgag ccgtcgtggc ccgtgttcat ggcttgcagc
     9001 atgtccagcg tttcgggtcc gcggcattcg ccgatgatga tccgctcggg acgcatacgc
     9061 agggcgtttt tcaccaggtc cgtggcggtg atcgcgccct tgccttcgat gtttggcggc
     9121 cgggtctcca gccgcacgat gtgatcctgc tggagctgaa gttcggccgc gtcctcgatc
     9181 gtgacgatgc ggtcggtgtt cgggatgaag ctcgacagcg tgttgagcag cgtggttttg
     9241 ccggaacccg tgccgccggc gatgatcatg ttcagccggg ccttgatcgc cccttccagg
     9301 agcatgacca tttcgggcgt cagggccttg aagttcaaca ggtcttcgag cttgagcgga
     9361 ttgctgccga aacggcgaat cgagaccgcc gcgccgtcca gggccagcgg cggaatgatc
     9421 gcgttcacgc gggagccgtc ggtcaggcgg gcgtccacca tcgggcagac ttcgtccacg
     9481 cgtcggccga ctttcgacac gatccggtcg atgatttgca gcaggtgagc gttgtcgcga
     9541 aactcgacgg aagtcttctc caacttgccg cggcgctcga catagatgtt cttcgggccg
     9601 ttgatcagga tgtcgctgat cgtgtggtcc ttgagcagca tttccagcgg ccccaggccg
     9661 aacgtttcgt cgaggacctc ctcgatcaag cgctcgcgct cggcccggtt gaggaaggtc
     9721 tcctcggtgt cgcagaggtg ctcgacgacc aggcggattt cccgccgcag cacgtcgcct
     9781 tggagctcgc cgacgcggtt cagatcgagc ttatcgacca gcttgttgtg gatttttcgc
     9841 ttgagctggt cgaactcttc ggccttcgcg gcgtcggccg tgcgggcagt cgtggccagg
     9901 ggcttcatcg cagcattccg gggacaacga gggggctagt tgcggagggc tgtacgccat
     9961 ccgccaaaaa catagctcac cactcttgcg ccgccggaaa tgtttgacgc gcgccccaga
    10021 ccgtctggcg cgatggaggc ggctttgccg gatttgtcac ttgtcactcg taatggtcat
    10081 ttgaagaggc caatgacaag tgaccaatga cagctcacac gaacttcgtc acccctggaa
    10141 tcgccaccgg gggctccggc agcttgatgt tccagtcgta ggccggcggg gtgagatctt
    10201 ccttggaatt gaaggccatg tcccaggtga tggcttcgcc ggtgtaggcg gccatccggc
    10261 ccatcacagc cagcagcgtg ctcttggcca tgtactcgcc gttgttgagc ggcttgccgt
    10321 tgcggatcgc ggcgaacagc tcgtcgtgct cggtctggta gaggttgttc tccggcccgt
    10381 cgtaggccca gtcgccggcg tcggtcttca gcagcatacc ccgcttgcgc tcgttgatgc
    10441 gggccgtgcc cttggtgccc agcacgtggg cgctcatgtc gccgacgcag ttgggaatct
    10501 gccggcagtt gctgaacacc ttcgtgccgt cgtcgtattc gtaggtgacg ctgaagtggt
    10561 cgtagatgtt gccgtagtcc ttgccggtgc ggacctgccg cccacccagt ccaacggcct
    10621 tcgtcggata ttggttcttc atgatccacg cgcagacgtc gaggaagtgc acgtgctgct
    10681 cgacgttgaa gtcgcccgac agccaggtga agtagtacca gttccgcatc tggtaggtca
    10741 tgtcgctcca gtcgggctgc cgcggcttga cccaaatgcc gccgcggtag tcgttggctt
    10801 gcagggccag cggcttgccg atctggccat cgtggatccg cttgacgatc tcctggaacc
    10861 gccagtcgta gcggaggcag aggcccgata cgaccgagag gttcttggct ttggcctgtt
    10921 cgcaaatgtc gagaactctc cgcacgcccg gcgcatcgac cgccaccggc ttctcggcga
    10981 agatgtgctt gccggcggcg accgcggctg ccaggtgcag cgggcggaac tgcggcggcg
    11041 tggtgagcag gaccacgtcg cagcaagcga tgacctcttt ataggcgtcg aagccgacga
    11101 accgccgttc cttggccacg tcgagcttgg gggcgacctc ctcgatcttc tgcaggctct
    11161 gcaggctgag atcgatgcgg tcctcgaagg cgtcccccat cgcccagagc cgcgccccgg
    11221 aatcggcctt gagggcctgc gacgccgcgc cgctgccgcg cccgccgcag ccgatcaggc
    11281 cgaccttgag cacgtcgctg cccgcggcgt ggacgcgcgg cgccagcgcg agcgtcgcca
    11341 gcgagccggc gacggcgagc gaggactttt ccaggaactg ccggcgagtg ttgttcgtca
    11401 tggatgattc ctattgtcgt ttaacccgga gccggaggcg acggctccgc gaacggcctg
    11461 cgccgcctgc gcctccggct acgggttaaa cgagactggc tggatttcga acttgcgaaa
    11521 gtaaatctcg tgcccttcgg tctggagcag aatccggccg gcggcgggaa acacctcgcg
    11581 gcactcgttg accgtctggc cgttgacctt gatcgtgagc ttgctgccgt cggcgatgca
    11641 ctcgacgcgg gtccagtcgc ccagcgggct ttcgacgtct tccttgccgc gggtgtcgag
    11701 cagctccttg aagtcccagt cgtgccgcga ccaccagagc tgcccgcgcg tgggcggaaa
    11761 cgtgagcgct tcgccccctt ccttccagcg acggcggcgg ccgtcgggcg cgagctccgt
    11821 ctcggccgtg acgcggacgg gaatcggttc cccctgctca tccttgccgc gaatcacgat
    11881 gatgtcgccg acgcagccct gggcgagctg gcactcgatg cagctcggcc acgccccgcc
    11941 cgcgccgcca tcggggccag tggcatgcag caggatgccc gaattgcgga cgtacttgcc
    12001 gccgtcggtc cgctggcccc acttgtattc gacgaccagc cggtagtcgc gatattcctt
    12061 ggccgtcgcg acgtagccgt tgccgccgcc cgtcaagtgg atcatcccct cggtgacgcg
    12121 gaagactcgg ctcggatctt cgcgctggct ctctttgagc cacgtcgtca gtccgctcag
    12181 gtcgcggccg ttgaagagct ggatcgcctg ccgcggagcg atcgcctggg tgccctcttg
    12241 ggcccacgcg acctggccgc aggctgcaag aattgccgtc agcgtcaccc agcgagccgc
    12301 agccatcaga attctccgcc accaatatcg tggttctgcc ctgtcgagtc aaatttccag
    12361 ttacattggt tcgagcaact cgttccagcc agccgtgtca aattcgtgag gagcggtcat
    12421 gtcgttcaaa gctgcaatcc tgcggggagc gctaaccatg tggatggttg tcggtgcggc
    12481 gacgagcgtc tcgtcgcaag acgtcggcac acaagcccag gaattcgtgc gcgagcacga
    12541 gcgcaagatc cagccgctgc aaatcgcgtc ggctcgcgcc tggtggaccg ccaacatcac
    12601 gggccaggac gaggattttg ccgccaagga agaggccgag aacaagatca acgaggcgct
    12661 ctccgacaag cagcagttca atcgcctgaa gcaaatccaa gctggaaaga tcgccgatcc
    12721 agtgctccgc cgcacgatcg acgtcctcta cctgcaatac ctcgacaagc aggtggaccc
    12781 tgcgctcttg cggcggatga ccgccaaggc caacgccatt gaaaaggcat tcaatgtgtt
    12841 ccgggcgcaa gtgaagggaa agtcgctcac cgacggcgag gtgcggcagg tcctccagaa
    12901 atccaaggac agccaggagc ggcaggccgt ctgggaagcg tccaagcgcg tcggcgcgga
    12961 ggtcgaaaac gagctgaaag acctggtgcg attgcgaaat gaagcggcca cggggctggg
    13021 ctttcccgat taccacgcgc tacagctgca cctgaccgag ctgacccagg cgcaggtcct
    13081 caagattttc gacgagcttg acgaattgac gcggcagccg ttcgaccggg ccaagtcgcg
    13141 gatcgacgaa aagctggccg cgagctacgc gctaaagccc cagcagctga tgccctggca
    13201 ctatcacgat ccgttcttcc aggaagcgcc ggcggtctac gaggccgatc tggaccagcc
    13261 attcgccgac gccgacatcc agcggatttg ccgcgagttt tacgccggca tcgggctgcc
    13321 gatcgacgac gtcctggcca agagcgatct gtacgagaag cggggcaaga gcccgcatgc
    13381 cttctgcacc gacatcgacc gcgcggggga cgtacgcgtg ctgggcaaca ttcgccccac
    13441 cgaatattgg atgagcacga tgctgcacga gttggggcac tcggtctatt cgagcaagaa
    13501 cattccggcc gagctgccgt gggccctccg cagcgacgct cacactctgt gcaccgaagg
    13561 gttggcgatg atgttcgagc gtttttccaa gagcgccgag tggctccgcg cctacggcgt
    13621 cgaggtcacc gagcccgaga aattcagcgc ctccgccgcg gaaatgcgcc gcgaccggct
    13681 gctgatcttc tcgcgctggg cacaggtaat gttccggttc gagaaggagt tgtaccggga
    13741 cccgtcgcag gatctgaata agctctggtg ggacctggtg gagaaatatc aaggtgtgcg
    13801 gcggccaccc gagcggaacg cccccgacta cgccagcaag atccacgtcg tctcggcccc
    13861 ggcttactac cacaactacc tgctgggcca gctcttcgcg tgccagttgc acgcgtcgat
    13921 cgccaaggag gtcctgaatc gcagcgatgc cgcagatgcg atctacaccc gcgaagcgcg
    13981 cgtgggaaag tacctgcgcg agaagctctt ctcgcaaggg gcgctgcgga gctggaacga
    14041 cgtcacgcgc tacgcgaccg gctcggagct gaatgccaag gcttttgccg ccgagctgca
    14101 agattgagac cgaccgcggt caatgtgaat atggacctgc aaacgctgct cggccaaacc
    14161 agcctgtcgc ggtttgtgct cgactattac cagcggctgc cgttttcggc cggcggcgct
    14221 ttccaggcgg cggcgctggc cgactggggc acgctgaccg cgctgctagg cgagagcggc
    14281 gcagacgttt tcatctgccg ccgtaacgag cgctacgcgg gcgatttgcc gcgcgatgcc
    14341 gccgcggcaa ccaggctggt ccaggaggga tacacgctgc tcgtgcggca cgccgagcgg
    14401 cacgacgctc gactcgcgcg gctcgcggcg gcattcgagc gcgatttcgc ttcggtcgtc
    14461 aacgtacacc tctactgcac gcccgccggc gagttcggct tcggctggca ctatgacgcc
    14521 gaggaagtgt tcatcgtgca gaccgtcggc cgcaagcaat acgccctgcg caagaacacc
    14581 gtgaacccct ggcccctgga agagacgctg cccgccgaca tgcagttcga gcgcgagatc
    14641 atgccgctgt ggcgctgcga tctccagccc ggcagctggc tctacattcc gtcgggctat
    14701 tggcaccggg ccgaggcccg cgagctgtcg atttcgctgg ccatcggcct ggcgccgcat
    14761 tccgccctgg acgtgtggga ctttctccgc cgcgagctgc tgcagtcgct cgagtggcgg
    14821 cagcgactgc cggtcctggg ccgcgcgtcg ccacttaccc aggacgagct gcgggccagg
    14881 tatgcgcagg tttttcaggc gctgggcagc gatctgtccc accggctcac agatcccggc
    14941 gccgttgaca aatacctgcg gtcgcgcggc ggcgagcgga ccgcggccga gttgtgaaca
    15001 ttggtacgct atttgcagta tagttccctt tggcgcgggc ggctgcc
//
LOCUS       DASRJB010000542        11186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3531216, whole genome shotgun sequence.
ACCESSION   DASRJB010000542 DASRJB010000000
VERSION     DASRJB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531216"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VFB80_14080"
     CDS             complement(<1..2078)
                     /locus_tag="VFB80_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN34952.1"
                     /translation="MPSPSLLHLILGAAVLLPLGSFVVILLCAKWLGDKAGLVATAAI
                     LSSGLLSFGAFGIWVYAHFPQPVHHEKHAEHPRPLASDHHPSPAPEGKHSRVADAPLN
                     FVSFAQEPAEKSKTADHPPAAAAASAEPESAAPGNAHHTERPVYTGEYYTLGQFGPLR
                     ITISYYIDALTIAMFCMVTLIATLIHVYAIGYMHDELSPVTDKEVVLSSGEVLHRPGR
                     YPRFFQYLSLFCFSMLGLVLSGNIAMVFVFWELVGICSYFLIGFYIERQSASNAANKA
                     FIVNRVGDFGMIIGLLVIWSTLGTFAFGDVDTDRDGTKEPGIFSLVRPEPNHALIVPD
                     EMVKADAYEEIAGVYSDHSTDADGLSRSQSEVASRVSGWRAKGYGYWLLVLAGVGIFC
                     GCVGKSAQFPLHVWLPDAMEGPTPVSALVHSATMVAAGVYLVGRFFPVFTPEVLLVIA
                     VIGTITMLLGASIAFAAYDIKRVLAYSTVSQLGLMMLALGVGGWAAGMMHLITHAFFK
                     SLLFMCSGSVIHAVHTNDLRKMGGLFNKMPVTAITMLIGCLAIAGVAVPFVVGFSGYY
                     SKDAILEQALSFWQFNGTPQAGVFFILAVFGAALTTCYMFRMWFLTFVGQPRDQHAYD
                     HAHESPPVMYLPLVILAIFAVSVAWNIALLGYGLIAAAFFILLGIQQGWFQRSAAAAH
                     GHDDHGHGHD"
     gene            complement(2078..2413)
                     /gene="nuoK"
                     /locus_tag="VFB80_14085"
     CDS             complement(2078..2413)
                     /gene="nuoK"
                     /locus_tag="VFB80_14085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZN34953.1"
                     /translation="MNPFTEPITVSHYLAAGAVLFVAGAICMATKRNALGVLMGIELV
                     LNGANLNFVAFGSKFLRTDTLGLDGELIALFVIVLAAAEAAVALAIALNFYNNHTTID
                     VDQADELKG"
     gene            complement(2419..3474)
                     /locus_tag="VFB80_14090"
     CDS             complement(2419..3474)
                     /locus_tag="VFB80_14090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZN34954.1"
                     /translation="MTLLLAAIHWHSVTFLFFALVACAFAVAVVATSNVVRMAFYLTL
                     SLGATSGLFFLAGAPFVGAMQLMIYVGGTLVLLIFGVMLTAQARFISMKTSGGEWVMA
                     AVLGASLLGLLVATGFSIESWRKPRADLAAVTLADGQTSTPIGMALTGVRVDKLSEPS
                     AARRHGMSGYLFPFVIISMHLLVVLIGAGYMARTKRVGSRMALERAAPATPRIRRRSF
                     AVAGGLISGIVVNLALAIACFAWWLRPPAAAASGNSLLGQIQILLEPASGWLWPVLGG
                     LFAANVLLLVIVYLWQSWAVIGLVAIPLAQAAAIANAGLGNVPAVAFALLALAPVVLL
                     IVLMCSGPRPTMWEQMD"
     gene            complement(3471..4853)
                     /gene="nuoH"
                     /locus_tag="VFB80_14095"
     CDS             complement(3471..4853)
                     /gene="nuoH"
                     /locus_tag="VFB80_14095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZN34955.1"
                     /translation="MAQFFNEELLHRLAGWFGSSVGPNFWLGYVLAAAVHIVLLIHVV
                     AVGALIFIWLERKVAGRIQDRLGPTRVGGKFGWLQTLADGIKLIAKEDITPKDADPLL
                     FKLAPYVSFAASFSAYMALPFANGWAAQEVNVAVFFIVAVLGVEIFGVILAGYASGSK
                     WSLFGAMREAAQVVSYEVPIGLCVVVPVLIVGSMNLVTIGEAQAGLLTNWLIFHDPAT
                     FCIFFVYFTCAVASVNRAPFDLAEAESELVAGFHTEYSGLRWSFFFMAEYGSMFLVSG
                     LAAILFYGGWNGPIPIFHWLPGFFGLEAAPGGGWAYVQGETSWRVLGYIAQVAGVLNF
                     IIKSCLFVIVMMWVRWTLPRLRIDQVMTTCLKYCVPLGAAFFVGAMFWKLLGIPFIND
                     IAPFRGQTLADVRENWVLVPEPVAAAKPASDEPQPMPEPAHPTPPAATTTTHHSPRTS
                     ATLTSQETAP"
     gene            complement(5041..5877)
                     /locus_tag="VFB80_14100"
     CDS             complement(5041..5877)
                     /locus_tag="VFB80_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34956.1"
                     /translation="MLPLIWLTSVSGLAAETPRVQFDMPFAVACREVTPPNFSLASPG
                     QKLIEVKLEISSLLVAGQERDLTQYFIRIENPARPLSVADYLPKTLHESRMSGPVTIT
                     DTTEKNASLGINVAGKYELISAIGATAGIGQKNTSCVKYNLLPPLEAVAASGTLNRGS
                     TVFFKLRATPRNVLEGTREYGLVLRVPSSWRADSMRVHCEAEGIQRSIVSTFNEEVSC
                     GRREFTVALYLEGDEQARLTAEDFARRKSADARNSGGKASSRGKSEPGWTLSSPWEML
                     RR"
     gene            complement(6102..7007)
                     /locus_tag="VFB80_14105"
     CDS             complement(6102..7007)
                     /locus_tag="VFB80_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN34957.1"
                     /translation="MSRLVVAAAILFVATSALRAADPPPDDGKLRIVVFGAHPDDAEY
                     KAGGCGAKWAKQGHHVLLCSVTNGDIGHWQMAGGPLAARRTAEVAAVSKILGTTSKVL
                     DIHDGELEPNLENRKKIIRVIREWKADVVISHRPWDYHPDHRYVGVLVQDAAYMVTVP
                     FICPDTPILTRNPVFLYSSDRFQKPYPFKADIAVSIDDVFDQKMDAIHEMASQHYEGG
                     ANGSEAHVRSVPPASDVAGRKAWLREQWFGRSGGEATRHRADLIQWYGEEKGKAVKYA
                     ECFEICEYGRQPNREEIKRLFPFFN"
     gene            7131..8345
                     /locus_tag="VFB80_14110"
     CDS             7131..8345
                     /locus_tag="VFB80_14110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN34958.1"
                     /translation="MTAANPPPPADSQPPAASPIPRNVLLLGFTSLLNDVASEMLFPL
                     LPTFMISVLKGTKEQLGLLDGLADTAASLLKLWSGASSDAAGRRKGFVIFGYGVAAIA
                     RPLMALAAAPWHLVALRTLDRIGKGVRTAPRDALIAESTSRESHGRAFGFHRAMDHLG
                     AAIGPLLAFAFLWYWPNQLRVLFALSLIPAIPILFIVIFGIREEPRPAHAKPKERFAL
                     SLAPFGAHFRLYLAALLLFTLGNSSDSFLLVRAGELGVPATWLPILWGVFHFAKSGLT
                     QAAGPWIDRLGPRPLILSGWLIYAATYLAFGLATQAWHAWSLFLVYAIYYALAEPAEK
                     ALVAGLVPAERRGLAFGWFNLVIGIGALPASLVFGSVYQRLGPLAAFGMGAGLALAAA
                     VLLASVPHKPEA"
     gene            complement(8421..9827)
                     /locus_tag="VFB80_14115"
     CDS             complement(8421..9827)
                     /locus_tag="VFB80_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34959.1"
                     /translation="MKPSPFDSPVSRREVLRRSAVGFGAIGLAGTLQAAGLLGSAQAA
                     GISRGHFAPRAKRVIYLFMNGGPSHVDTFDPKPALKEHEGEIPGGDNGRKKKGGFIPS
                     PFAFAPQGQSGVVMSELFPNIARCADDLCVIRSMHTDVPNHEPGLLIMNCGNQQPIRP
                     SLGSWISYGLGSENENLPAFVVLATRKPVVGPALWSNSFLPAAYQGMSVDTSEMEISK
                     LVTNLHHPTLGRIEQRQQLDLLAQLNQLHLQQRRDSALEAQIQAMETAFQMQAEAAEA
                     FDISREPEALREAYGKTNFGQSCLLARRLCEHGVRLVQVFYLSKDNNQPWDTHSNNDD
                     GHRKLCKDADQATAALLTDLKQRGLLEDTLVIWGGEFGRTPYAEKQEKAKPGRDHHHT
                     GFSMFLAGGGVKGGLTYGSTDEFGMHAVENRVHVHDLHATILHLLGLDHERLTYHYSG
                     RDFRLTDVHGRVVRDILA"
     gene            complement(9836..>11186)
                     /locus_tag="VFB80_14120"
     CDS             complement(9836..>11186)
                     /locus_tag="VFB80_14120"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34960.1"
                     /translation="IAALNRDLPYDEFVRLQLAADLLQGEDTPNLPALGLIGLGPKYY
                     SRRSLQVMSDEWEDRVDIVGRGLLGLTVACARCHDHKFDPIGTEDYYALAGVFASTSM
                     FNRPLTAEAARKEDDKNDTKEFENGKQVVGEAKDPKQALHIVRDGEPKNLNIFVRGNV
                     DTKGPEVPRHFLRVLAGDDPRAFTSGSGRRELAEAIASPENPLAARVIVNRLWALYFG
                     KPLVSSPSNFGAHGERPTHPELLDDLAVRFIESSWSLKWLTREIVLSAAYRQASTATE
                     VTLAADPENRLLSRMNRRRLTVEQWRDALLAAGGKLESAIGGPSLDPQKPEERRRTVY
                     ARASRLELNKLLAMFDYPDPNVHADRRVETTTPLQKMFVLNSPFMVAQSAAFAERLAS
                     EVDSDDRARIERAYLLLYGRPAMEKEIRLGLDYLSGQDRWQQYAHVLLAANEMLYLD"
BASE COUNT         1973 a         3721 c         3618 g         1874 t
ORIGIN      
        1 tcatcgtgac cgtgcccgtg atcgtcgtgg ccatgtgccg ccgcggcgga gcgttggaac
       61 cagccctgct gaattcccag cagaatgaaa aacgcggcgg cgatcaggcc atagccgagc
      121 agggcgatgt tccaggcgac gctgacggca aaaatggcca ggatcaccag cggcaggtac
      181 atcaccggcg gcgattcgtg ggcgtggtca taggcgtgct ggtcgcgcgg ctggccgacg
      241 aaggtcagga accacatccg gaacatgtag caggtcgtca gcgccgcgcc gaacaccgcc
      301 aggatgaaga acacgccggc ttgaggcgtg ccgttgaatt gccagaacga gagggcctgc
      361 tccaggatcg cgtccttgga gtaatagccg ctgaagccga ccacgaaggg cacggcgacg
      421 ccggcgatcg ccaggcagcc gatgagcatc gtgatcgcgg tgacgggcat tttgttgaac
      481 aggccgccca tcttccgcag gtcgttggta tggacggcgt gaatcaccga gccggagcac
      541 atgaacagca ggctcttgaa gaaggcgtgc gtgatcaggt gcatcatgcc ggccgcccag
      601 ccgccgacgc ccagggccag catcatcagt ccgagctggc tgaccgtcga ataggctagc
      661 acgcgcttga tgtcgtaggc ggcgaaggcg atcgacgccc ccagcagcat cgtgatcgtg
      721 ccgatgaccg cgatgacaag caagacctcc ggcgtgaaga ccgggaagaa gcggcccacg
      781 agatacacgc cggcggcgac catcgtcgcc gagtggacca gcgccgagac cggcgtcggt
      841 ccttccatgg cgtccggcaa ccagacgtgg agcgggaact gggcactctt ccccacgcag
      901 ccgcagaaga tgccgacgcc agccagcacc agcagccagt aaccgtaacc cttggcccgc
      961 cagccgctga cccgcgaggc aacttccgat tgcgagcggc tgagtccgtc cgcgtccgta
     1021 gagtggtcgg aatagactcc ggcgatttcc tcgtaggcgt cggccttcac catttcatcc
     1081 ggcacgatca gcgcatgatt tggctcgggg cggaccaggg aaaagatccc cggttccttt
     1141 gtgccgtcgc ggtcggtatc gacgtcgccg aaggcaaacg tgcccagcgt ggaccagatc
     1201 accaacaggc cgatgatcat gccgaagtcg ccgacgcggt tgacgatgaa cgccttgttg
     1261 gcggcgttgc tcgcgctctg tcgctcaatg taaaagccaa tcaggaaata ggagcagatg
     1321 cccaccagct cccagaacac gaacaccatc gcgatgtttc cggacagcac caggccgagc
     1381 atgctgaagc agaacagcga caggtactga aaaaaccgcg ggtagcggcc gggccggtgc
     1441 agcacttcgc cgctcgagag gaccacttcc ttgtcggtca ccggcgacag ctcgtcgtgc
     1501 atgtagccga tggcgtagac gtggatcaag gtggcgatca acgtcaccat gcagaacatg
     1561 gcgatcgtca gggcgtcgat gtagtagctg attgtaatcc gcaacgggcc gaactggccc
     1621 agcgtgtaat actcgcccgt atagaccggc cgctcagtgt gatgggcgtt tcccggtgcc
     1681 gccgattcag gttcggcgct cgccgctgcc gcagccgggg ggtgatccgc ggttttgctc
     1741 ttctccgctg gctcctgggc gaaactgacg aagttcagcg gagcatcggc gacgcggctg
     1801 tgcttgcctt cgggcgcggg cgacgggtga tgatcgcttg ccagaggacg cggatgttcg
     1861 gcgtgcttct cgtgatgcac cggctgcgga aagtgggcgt acacccagat gccaaaggcc
     1921 ccgaacgaga gcaagcccga ggagagaatt gccgctgtcg ccaccagacc cgccttgtcg
     1981 cccagccact ttgcgcagag caaaatgacc acgaacgagc ccagcggcag gagcaccgcc
     2041 gcgccgagga tcaaatgcag cagggaaggg gagggcatca gcctttgagc tcgtccgcct
     2101 gatcgacgtc gatcgtggtg tggttgttgt aaaagttgag ggcgatggcc agggcgacgg
     2161 cggcctccgc ggcggccagc acaatcacga acagggcgat caattcgccg tcgaggccca
     2221 gcgtgtcggt ccgcaggaac ttgctgccga aggccacgaa gttgaggttc gcgccgttga
     2281 gcacgagctc aatccccatc agcacaccca gggcgttccg cttcgtcgcc atgcagatcg
     2341 cgccggcaac aaacagaacc gccccggctg ccaggtaatg cgaaacggtg atgggttcgg
     2401 taaagggatt cattccggct agtccatttg ctcccacatc gtcggccgcg ggcccgagca
     2461 catcagcacg atcaagagca cgacaggcgc taaagccagc agggcgaagg cgaccgcggg
     2521 cacgttaccc agacctgcat tggcgatcgc ggccgcctgg gccagcggaa tcgccaccag
     2581 cccgataacc gcccacgact gccagaggta aacgatgacg agcaaaagca cattcgccgc
     2641 gaacaggccg cccagcaccg gccagagcca accggatgcc ggctccagga gaatctgaat
     2701 ttgaccgagc agcgaattac cgctcgccgc tgccgcgggc ggcctaagcc accaggcaaa
     2761 acaggcaatg gccagcgcca ggttgacgac gatccccgaa atgagcccgc ccgcgacggc
     2821 aaacgaccgg cggcggatgc gcggcgtcgc cggggccgcc ctctccaggg ccatccgcga
     2881 gccaacccgc ttggtgcggg ccatgtagcc cgcgccgatc aatacgacca agaggtgcat
     2941 cgagatgatc acgaacggaa acaggtagcc cgacatcccg tgccggcggg ccgcacttgg
     3001 ttcgctgagc ttatcgacgc gcacgcccgt caaagccatg ccgatgggcg tgctcgtctg
     3061 gccgtcggcg agcgtgaccg cggccagatc ggcccgcggt ttcctccacg actcgatgct
     3121 gaagcccgtc gccaccagca gccccaagag actcgcgccg agcaccgccg ccatcaccca
     3181 ttcgccgccg ctggtcttca tcgagatgaa gcgggcctgg gcggtgagca tgacgccgaa
     3241 aatcaagagc accagcgtgc cgcccacgta gatcatcaac tgcatcgcgc cgacgaacgg
     3301 cgcgccggcc aggaagaaca ggccggaggt cgcgcccagc gaaagcgtca ggtaaaacgc
     3361 catccgcacg acgttgctcg tcgccaccac cgccacggcg aacgcgcacg cgaccagtgc
     3421 gaaaaacagg aacgtcaccg agtgccagtg gatggcggcc aggagcagcg tcatggcgcg
     3481 gtctcctggg aggtcagggt ggcggaagtg cgtggtgagt ggtgagtggt cgttgtggcg
     3541 gcgggcggcg tgggatgcgc aggttcaggc attggctggg gttcgtccga ggcgggcttg
     3601 gcggctgcga ccggttccgg cacgagcacc cagttctccc gcacgtcggc cagcgtttgc
     3661 ccgcggaagg gggcgatgtc gttgatgaac ggaatgccca gcagcttcca aaacatcgcg
     3721 ccgacgaaga acgccgcccc cagcggcacg cagtacttca ggcaggtcgt catcacctgg
     3781 tcgatccgca aacgcggcag cgtccagcgg acccacatca tcacaatcac aaacaggcag
     3841 gacttgatga tgaaattcag cacgccggcg acttgtgcga tgtagccgag gaccctccag
     3901 cttgtctcgc cttgcacgta ggcccagccc ccgcccggcg cagcttccaa gccgaaaaag
     3961 ccggggagcc aatggaaaat cgggatcggc ccgttccagc cgccgtagaa caggatcgcc
     4021 gccagcccgc tgaccaggaa catcgagcca tattcggcca tgaagaagaa gctccaccgc
     4081 aggccggagt actcggtgtg gaagccggcg acgagctcac tctcggcctc ggccaggtcg
     4141 aacggggcgc ggttcacgct ggcaacggcg cacgtgaaat agacgaagaa gatgcaaaac
     4201 gtcgcggggt cgtggaagat cagccagttc gtcagtaggc ccgcctgggc ctcgccgatc
     4261 gtcaccaggt tcatcgagcc gacaatcagc accggcacca caacgcacag gccgatcggc
     4321 acctcatagc tcacgacttg ggccgcttcg cgcatcgcac cgaacagcga ccattttgag
     4381 ccggacgcat agccggccag gatcacgcca aagatctcga cgcccagcac ggccacgatg
     4441 aagaacaccg cgacgttgac ttcctgggcc gcccagccgt tggcgaaggg cagcgccatg
     4501 tacgccgaga agctggcggc aaaactcacg tacggggcga gcttgaacag cagcggatcg
     4561 gcgtccttcg gcgtgatgtc ttccttggcg atcagcttga tgccgtcggc cagcgtttgc
     4621 agccagccaa acttgccgcc gacgcgcgtc gggccgaggc ggtcctgaat gcggccggcg
     4681 acctttcgct ccagccagat aaagatcagc gccccgaccg caacgacgtg gatcagcagg
     4741 acgatgtgca ccgcagccgc gaggacatag cccagccaga aattcgggcc gacggaactg
     4801 ccgaaccaac cggccagccg gtgcagtagt tcttcgttga aaaactgtgc cactgtcgtc
     4861 gcccagcggg aggccgccct gcgacggaaa ccttgccgca gcaagccttt ccgtcgctat
     4921 gccaggtgcc ggtgcgccgt gacagcaccc ttgccggccc caaagatcgt gaaatatggc
     4981 acaaagtggt ccgcaccggc aagcccccgg ctggccaatt tcggctttcc cttgctaact
     5041 ctaccggcgc agcatttccc acggagagct gagcgtccaa cccggctcgg actttccgcg
     5101 gctgctggcc ttgcctcccg agtttcgagc gtcggccgac ttccgccgcg cgaagtcctc
     5161 ggcggtaagc cgcgcctgct cgtcgccttc caaatagagc gcgacggtga actcccgccg
     5221 tccgcagctc acctcttcgt tgaacgtgct gacgatgctc cgctggatgc cctcggcctc
     5281 gcaatgcacc cgcatgctgt cggcccgcca cgacgagggc acgcgcagca cgaggccgta
     5341 ttcccgcgtg ccttccagca cgttgcgggg cgtcgcacgc agcttgaaaa acaccgtgct
     5401 gccgcggttc agcgtgccgc tggcggcgac ggcctccagc ggcggcagca ggttgtactt
     5461 cacgcacgac gtgttcttct gaccgatccc ggccgtcgcg ccgatcgcgg aaatgagctc
     5521 atatttgccg gccacgttga tccccagcga cgcgttcttc tcggtggtat cggtgatcgt
     5581 caccggcccg ctcatgcggc tctcgtgcag cgtcttgggc agataatcgg caaccgacag
     5641 cggccgggcg ggattctcga tccggatgaa gtactgcgtc aggtcgcgct cctggccggc
     5701 gaccagcagc gacgaaatct ccagcttcac ttcgatcagt ttctggcctg ggctggccag
     5761 tgaaaaattc ggcggcgtca cctcgcggca ggccaccgca aacggcatgt cgaactgcac
     5821 tcgcggcgtc tcggccgcca ggcccgaaac ggacgtgagc caaatcaagg gcaacatcat
     5881 tccgctacaa acgcgctcca tcgatcgaga ctcctcagga tgaacagcgg ccctgcgctc
     5941 ctcctgcgct gccgggcggg gaccgtccag aaccggtccc cgcggtctcc ttcctggagc
     6001 catagaatcc cggagccccg cgccgccgcg ggccccgaac agtcgaatct tagaaggcgg
     6061 gctgccgcag tcaagtttgt ccagtgctag ctgccgccac tctagttgaa gaacgggaac
     6121 agccgcttga tttcctcgcg gttgggctgg cggccgtatt cgcagatctc gaagcactcg
     6181 gcgtacttca cggccttgcc tttctcctcg ccgtaccact ggatcaggtc ggcgcgatgg
     6241 cgggtggcct cgccgccgct gcggccgaac cactgctcgc ggagccaggc cttgcggccg
     6301 gcgacgtcgc tggccggcgg caccgagcgg acgtgggcct cgctgccgtt ggccccgcct
     6361 tcgtagtgct gcgaggccat ttcgtgaatc gcgtccatct tctggtcgaa cacgtcgtcg
     6421 atcgagaccg cgatgtcggc cttgaacggg tacggcttct ggaagcggtc gctcgaatag
     6481 aggaacaccg ggtttcgcgt gaggatcggc gtgtcggggc agatgaacgg caccgtgacc
     6541 atgtaggccg cgtcctgcac cagcacgccg acgtagcggt ggtcggggtg atagtcccac
     6601 ggccggtgcg agatcacgac gtcggccttc cactcgcgga tcacacggat gatcttcttg
     6661 cggttctcca ggttcggctc caattccccg tcgtggatgt cgagcacttt cgaggtcgtg
     6721 cccaggatct tcgagaccgc cgcgacctcc gcggttcgcc gcgcggccag cggcccaccg
     6781 gccatctgcc agtggccgat gtcgccgttg gtcacgctgc agagcagcac gtggtggccc
     6841 tgcttggccc acttcgcgcc gcagccgccg gccttgtatt ccgcgtcgtc cgggtgcgcc
     6901 ccgaagacga caatccgcag cttgccgtcg tccggcggcg gatcggcggc gcggagggct
     6961 gacgtggcaa cgaataggat tgcagcggca acaacaagac gggacatggt gtggttcctc
     7021 gacgggacaa gtcagtgata atcgcccgta ctctgtactg cgtactgagt actcgctacc
     7081 gagtacggag tatagccgcg cccaacgtcg cacctcaaag ccgccgatca ttgaccgccg
     7141 ccaatccacc gccgcccgcc gattcgcagc cgccggccgc ttcgccgatc ccgcgcaacg
     7201 tcctgctcct gggcttcacc agcctgctca acgacgtggc cagcgagatg ctctttccgc
     7261 tcttgcccac gttcatgatc tcggtcctca aggggacgaa ggaacagctc ggacttttgg
     7321 acggcctggc cgacaccgcc gcgagcctgc tcaagctctg gtcgggtgcg agctccgacg
     7381 ccgccggtcg ccgcaagggc tttgtcatct tcggctacgg cgtggccgcg atcgcccggc
     7441 cgctgatggc cctggccgcc gcgccctggc acctggtcgc gctgcggacg ctcgaccgca
     7501 ttggcaaagg cgtccgcacc gcaccgcggg atgcgctcat cgccgaatcg acgtcccgcg
     7561 aaagccacgg ccgcgccttc ggctttcacc gggcgatgga ccacctcggc gcggccatcg
     7621 gcccgctctt ggccttcgcc tttctctggt actggccgaa ccagctgcgg gtgctgttcg
     7681 ccctttcgct aatcccggca attcccatct tgttcatcgt gatcttcggc attcgggagg
     7741 agccgcggcc ggcgcacgcc aagccgaagg agcggttcgc cctttcgctc gccccttttg
     7801 gcgcccactt tcggctttac ctcgccgcgc tcttgctctt cacgctcggc aactccagcg
     7861 acagctttct cctcgtgcgg gccggcgagc tgggcgtgcc cgcgacgtgg ctgccgatct
     7921 tgtggggcgt gttccatttt gccaagagcg gcttgacgca ggccgccggg ccgtggatcg
     7981 accggctcgg cccgcggccg ctgatcctgt ccggctggct gatctacgcg gcgacttacc
     8041 tcgctttcgg cctggcgacg caggcttggc acgcctggtc gctgttcctg gtctatgcga
     8101 tctactacgc gctggccgag cccgccgaaa aggcgctcgt cgccggcctc gtgcctgccg
     8161 agcgccgcgg cctggccttc ggctggttca acctggtcat cggcatcggc gctttgccgg
     8221 ccagcctggt cttcggcagc gtctatcagc gcctcggccc gctggcggcc ttcggcatgg
     8281 gggccggcct ggcccttgcc gccgcggtgc tgctggcgag cgtgccacac aaacccgaag
     8341 cgtaagagcg taagcgaggg gcattctcga taccgcccga acttaggaag tccctcgctt
     8401 acgctcgggt tgctgtcctg ctaagccagg atgtcgcgca cgacccgccc gtgcacgtcg
     8461 gtcaggcgga agtcccggcc gctgtagtgg tacgtcagcc gctcgtggtc gaggcccaag
     8521 aggtgcagga tcgtggcgtg cagatcgtgg acgtggacgc ggttctcgac ggcgtgcatg
     8581 ccgaactcgt cggtcgagcc ataggtcagg ccgcccttca cgccgccgcc agccaggaac
     8641 atcgagaatc ctgtgtggtg gtggtcgcgg ccgggcttgg ccttctcctg tttctcggca
     8701 tagggcgtgc gtccgaactc gccgccccag atgacgagcg tgtcttccag gagcccgcgc
     8761 tgcttgagat cggtcagcag cgccgccgtc gcctggtcgg catccttgca cagcttgcgg
     8821 tgaccgtcgt cgttgttgct gtgcgtgtcc cagggctggt tgttgtcctt cgacagatag
     8881 aacacctgca ccagccgtac gccgtgctcg cacaagcggc gggccaggag gcacgactgc
     8941 ccgaagttcg tcttgccgta ggcctcgcgg agcgcctccg gctcgcggct gatgtcaaag
     9001 gcctcggccg cctcggcctg catctgaaac gccgtctcca tggcttggat ctgcgcctcg
     9061 agggccgagt cgcggcgctg ctgcaggtgc agctggttga gttgggcgag caaatcgagc
     9121 tgctgccgct gctcgatgcg gccgagcgtc ggatggtgca ggttagtaac cagcttgctg
     9181 atctccatct cgctggtatc gaccgacatg ccctgatagg cggccggcag gaatgagttc
     9241 gaccagaggg ctgggcccac caccggcttg cgcgtggcca gcacgacaaa cgcgggcaga
     9301 ttctcgttct cgctccccag gccgtagctg atccaggaac ccagcgaagg gcggatcggc
     9361 tgctgattgc cgcagttcat gatcaagagg cccggctcgt ggttcggcac gtcggtgtgc
     9421 atcgagcgga tgacgcacag gtcgtcggcg cagcgggcga tgttcggaaa cagctcgctc
     9481 atcacgacgc cgctctggcc ctgcggggcg aacgcgaacg gcgaggggat gaaaccgccc
     9541 ttcttcttgc ggccgttgtc gccgccgggg atttcgccct cgtgttcctt gagggccggc
     9601 ttggggtcga acgtatcgac gtgggacggc ccgccgttca tgaacaggta gatcacccgc
     9661 ttggcccgcg gggcaaagtg gccgcgcgaa atgccggcgg cctgcgccga gcccagcaag
     9721 cccgccgcct ggagcgtgcc cgccagcccg atcgcgccaa agccgaccgc cgagcggcga
     9781 aggacctcgc ggcgggaaac cggcgagtcg aagggcgaag gtttcatcgc gcacctcaat
     9841 cgagatagag catttcgttc gcggccagga gcacgtgggc gtattgctgc cagcgatcct
     9901 ggcccgacaa atagtccagt cccaggcgaa tctctttttc catcgccggc cggccgtaga
     9961 gcagcaggta ggcccgctcg atccgcgccc ggtcgtcgga atcgacctca ctcgccagcc
    10021 gctcggcaaa cgccgccgac tgggccacca tgaacgggct gttgagcacg aacatcttct
    10081 gcagcggcgt cgtcgtctcg acgcggcggt cggcgtgcac gttgggatcg ggatagtcga
    10141 acatcgccag cagcttgttg agctccagcc ggctggcgcg ggcatagacc gtccgcctcc
    10201 gctcctcggg cttctgcgga tcgagcgacg gcccaccgat tgccgactcg agcttgccgc
    10261 cggcggccag caaggcgtct cgccactgct cgaccgtcag gcggcggcgg ttcatccgcg
    10321 agagcaggcg gttttccgga tcggcggcga gtgtgacctc agtggccgtg ctcgcctgcc
    10381 ggtaggcggc gctgagcacg atctcgcggg tcagccactt cagcgaccag ctggactcga
    10441 tgaaccgcac cgccaggtcg tcgagcaatt ccggatgcgt cggccgctcg ccgtgtgcgc
    10501 cgaagttgct ggggctggaa accagcggct tgccgaaata cagcgcccac aggcggttga
    10561 cgatcacgcg ggctgccagc ggattctccg gcgaggcgat cgcctcggcc aactcgcggc
    10621 ggccgctgcc gctggtaaac gcgcgcggat cgtcgcccgc gagcacgcgg aggaaatgcc
    10681 gcgggacttc cgggcccttc gtatcgacgt tgccgcggac gaagatgttc aggttcttgg
    10741 gctcgccgtc gcggacgatg tgcagggcct gcttgggatc cttggcctca ccgacgacct
    10801 gcttgccgtt ctcgaactcc ttggtgtcgt tcttgtcatc ttccttccgc gccgcttcgg
    10861 cggtgagcgg tcggttgaac atcgacgtgc tggcgaacac gccggccagg gcgtagtagt
    10921 cctcggtgcc gatcgggtcg aacttgtggt cgtggcagcg ggcgcaggcc accgtcagcc
    10981 cgagcaaacc gcggccgaca atgtccaccc ggtcctccca ttcgtccgac atcacctgca
    11041 ggctgcggcg gctgtagtat ttgggtccca ggccgatcaa gcccagcgcg ggcaaattcg
    11101 gcgtatcttc cccctgtagc aaatctgccg cgagctgcag gcgcacgaat tcgtcgtagg
    11161 gcaggtcgcg gttgagcgcg gcgatg
//
LOCUS       DASRJB010000543         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1839330, whole genome shotgun sequence.
ACCESSION   DASRJB010000543 DASRJB010000000
VERSION     DASRJB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839330"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFB80_14125"
     CDS             complement(<1..420)
                     /locus_tag="VFB80_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZN34961.1"
                     /translation="MATQEGQHAARIRENLAAVRDRVAAAAARAGRRDLPRIVGITKY
                     VEPAVARLLAQAGLNELGESRPQELWRKAAALADMQVRWHVVGHLQRNKVRRTLPLVA
                     SIDSADSLRLLEEISREAVSQNLTANVLLEVNVSGEPA"
     gene            1078..1344
                     /locus_tag="VFB80_14130"
     CDS             1078..1344
                     /locus_tag="VFB80_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN34962.1"
                     /translation="MDRETLRTTLLDVLEQETWERPENAADHVKIREGLKLDSVDLLS
                     VMLRIETQLGISLESRDFEKVVTVGDLLTVIQAKLAAKSQSKAA"
     gene            1417..2235
                     /locus_tag="VFB80_14135"
     CDS             1417..2235
                     /locus_tag="VFB80_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34963.1"
                     /translation="MSVSQRTTCAALSFGLSSWGVAQAEYSAIGPLRKQLPDWSAAGT
                     AGHFLKHADEQTVMAVQAVDRAIREQHLDVREQANWPIVSAPRFPGRLAGIDSLKRFT
                     TGGGPALSPQLIAQHSLHSVSGALSVLIGSRAQNLGVGGGADSLREGLLTALTMARPR
                     GASGAWLVLTAYSPEPVIADDGAIQNRPVCQAAALALSFAAGASHCGRLSLRGDVGTP
                     AAIYEERPLALAELVASLQQLAAGAAARFTWRLDWGATIALEVRQSAARLAAAA"
     gene            2255..3466
                     /locus_tag="VFB80_14140"
     CDS             2255..3466
                     /locus_tag="VFB80_14140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN34964.1"
                     /translation="MRACPVVITGVGAATPLGADFDTFAANLLAGRSPAKAVTDSAAG
                     VEVRLPACLSDDPPAPVGWDEAEFRSLPRTEQFVLWCCTAALADAGLLAEDGARIGLS
                     LGAGGEWIRRWEGDWAAGGREVYEGRTNSPSLVDVAADRLSLNGPRATVAAACASGNA
                     AIAQARRWIELGLADICLAGGVETITPICRSGFNNLRALSKRVDDARRASRPFDQDRD
                     GFVIGEGAAILVLESSERARRRGARAYAEVAGFGATSDAFHMIIPSSDPAPAAAAMQL
                     ALADAAIEPHEIDYINAHATSTPVGDKAEARALHSVFGSHTATTPVSSTKSVTGHLLS
                     AAAAIEALACLVAIREQAIPPTINLERPDPECDLCHVPHQAIAHPVEYALSNSFGFGG
                     SNTSLILRKAA"
     gene            complement(3467..5107)
                     /locus_tag="VFB80_14145"
     CDS             complement(3467..5107)
                     /locus_tag="VFB80_14145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZN34965.1"
                     /translation="MNASELFVKALESERVQYVFGIPGEENLELLDALSRSSIRLIVT
                     RHEQAAGFMAATYGRLTGKAGVCLSTLGPGATNLVTAAAYAQLGGMPMLMITGQKPIK
                     TSKQGQFQLVDVVDMMRPLTKSTRQIVSGGSIPSRVREAIRLAEEERPGATHLELPED
                     VAREETSEPLLSASYVRRPVAEEKAIRAAVSRIEQSRHPLLLVGAAANRRTTSRMLRR
                     LIETTGIPFVTTQMGKGVVDETHPLFLGTAALSSGDFVHRAVEAADLIINVGHDVVEK
                     PPFIMRAGGVQVIHVNFFTAEVDPVYFPQVEVVGDIANSIWQLTERLSKPGHWDFSRF
                     ERIGAALEEHVRQGADDPRFPIYPQRLVADVRRAMQDDGILALDNGIYKLWFARNYQA
                     RQPNTVLLDNALATMGAGLPSAMAARLVYPGRQVMAVCGDGGFLMNSQELETAVRLKM
                     NLVVLIVRDDGYGMVKWKQVHQGFADFGLDVGNPDFVKYAESYGARGRRVERAADLLP
                     LLEECHRASGVQLIDVPIDYSDNDRILHHEIAERSAAI"
     gene            complement(5215..>5612)
                     /locus_tag="VFB80_14150"
     CDS             complement(5215..>5612)
                     /locus_tag="VFB80_14150"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34966.1"
                     /translation="QRLWSTNYPHNTDSGAEFYGTKGQMYLSRRGKIQVQADGNKPVD
                     VPVQPGAQDDASHVRNFCEAVRGEAKLNAPALTGHLSTSLCHLGNIATRLGRSLNFDR
                     ETEQIVGDDEANALVRRTYREHWGKPKGA"
BASE COUNT          842 a         1862 c         1940 g          968 t
ORIGIN      
        1 cgctggctct cccgagacgt tcacttccaa cagcacgttg gcagtcaggt tctgcgacac
       61 cgcctcgcgg ctgatctcct ccagcagtcg caggctgtcg gccgagtcga tggaagccac
      121 cagcgggagc gtgcggcgga ccttgttgcg ctgcaggtgg ccgacgacgt gccagcggac
      181 ctgcatatcc gccagggccg ccgccttccg ccacaactcc tgcggccggc tctcgcccag
      241 ttcgttgagc cccgcctggg ccagcaagcg ggcgactgcg ggctcgacgt atttcgtgat
      301 tccgacgatc ctcggcagat ccctccgccc agcgcgggcg gctgccgccg cgacccggtc
      361 gcgcaccgcc gccaggtttt ctcgaatgcg ggcggcgtgc tggccctctt gggtcgccat
      421 cccgccatcc taatgcaggc ggcgatggaa aaccgggcgc cacgcctggc gcgggctcgc
      481 gcgggcggcg gaggagaatt gtgatgtttg gtacactatt tgcagtatag ttctccttgg
      541 cgcagtgggt gccattttgg ctccccgctg ccagccgata cgctctctcg caattgggtc
      601 gttggcattt ggggcagaat cgccgccgct acgttttgct gcaaagggta aggataattc
      661 ccggtccgtc tctctagtgc ttctaatgca atgccgccaa accgcccgca ttccgcccga
      721 agtcctcaac tggcaggcag ttgtggcgac tggcggcgca gaggggagcg aaaaaaactg
      781 gcgctctcgg gaattatcct taccctcgcc ggcgtgcgtt tagggcagcc gctggaatag
      841 gaccgtctcg cgggtatgcc cggccggcgg tgaccatgcg agtcggccag agtggtacgg
      901 tcgggagacg gcgggtcggc aattggcacg cccacgcggc gtgggcaccc cggcgttatg
      961 gtctcaaatc cgtctttgtc cgcggcggcg gctttgatag tcttccggtc cgcaaatcgg
     1021 cggcgggcga ggatgctagc agccgcccgc atttccaccg tccccgaagg tgaagcgatg
     1081 gatcgcgaga cgctacggac gacgctgctc gacgtgctgg agcaggagac gtgggagcgg
     1141 cccgagaacg ctgccgacca cgtcaagatt cgcgaagggc tgaagctcga ttcggtcgat
     1201 cttttgagtg tgatgctgcg gattgagacg cagctcggca tcagcctgga aagccgcgat
     1261 ttcgagaagg tcgtgacggt cggcgatttg ctgacggtga ttcaggccaa gctggccgcc
     1321 aagtcgcagt cgaaagcggc ctgaagcgaa agatgagcgg gagcttctgt tcccgccggc
     1381 ggccgaccgc agccgccgcc ctgtgggagg ctcagcatgt cagtcagcca acgcacgacg
     1441 tgtgccgcgc tgtccttcgg gctcagctcc tggggcgtgg cccaggcgga gtactcggcg
     1501 atcggcccgc tccgcaagca gctgcccgac tggtccgcgg cgggcacggc tgggcatttc
     1561 ctcaagcatg ccgacgagca aaccgtgatg gccgtgcagg cggtcgatcg agcgatccgc
     1621 gagcagcacc tggacgtccg cgagcaggcc aactggccga tcgtttccgc cccgcggttt
     1681 cccggccggc tggccgggat cgattcgctg aagcggttca ccaccggcgg cgggccggcc
     1741 ctctcgccgc agctgatcgc ccagcattcg ctgcacagtg tcagcggggc gctgagcgtg
     1801 ctgatcggca gccgggcgca aaacctgggc gtaggcggcg gcgctgactc gctgcgcgaa
     1861 gggctgttga ccgcgctgac gatggcccgg ccccgcgggg ccagcggcgc atggctggtg
     1921 ctcacggcgt acagcccgga gccggtgatt gccgacgacg gcgcgattca gaaccggccc
     1981 gtgtgccagg cagcggcgct ggccctgtcg tttgccgcgg gggcgagcca ctgcggccgg
     2041 ctcagcctgc gtggcgacgt cggcacaccc gcggcgatct acgaagagcg gccgctggcg
     2101 ctggccgagc tggttgccag tctccagcag ctcgctgccg gcgctgccgc acgattcacc
     2161 tggcggctgg actggggcgc gacgatcgcg ctggaagtcc gccaatccgc ggctcgcctg
     2221 gcagctgcgg cctaactcgt tcaggaccat cgacatgcga gcctgccccg tcgtcatcac
     2281 cggagtcggc gcggcgacgc cgctgggggc tgacttcgat acgtttgccg cgaacctgct
     2341 cgcgggacgc agcccggcga aggccgtcac cgattccgcg gcgggcgtgg aagtccgctt
     2401 gccggcgtgt ttgtccgacg atccgccggc ccctgttggc tgggatgaag cggagttccg
     2461 ctctctgccg cggaccgagc aattcgtgct ttggtgctgc accgcggcgc tggccgacgc
     2521 gggcctgctg gccgaggacg gcgcgcggat tggcctgtcg ctcggagcgg gcggcgagtg
     2581 gatccgccgt tgggaagggg attgggccgc gggcggacgc gaagtttatg aagggcgcac
     2641 caactcgccg tcgctggtcg atgtcgcggc cgatcggctg tcgctgaatg ggccgcgagc
     2701 gacggtagcc gccgcctgcg ccagcggcaa tgccgcgatc gcgcaggccc gccgctggat
     2761 cgagctgggc ctggctgaca tttgcctggc gggcggcgtc gagacgatca cgccaatctg
     2821 ccgctcgggg ttcaacaacc tgcgggcgct gtcgaagcgc gtggacgacg cccggcgggc
     2881 cagccgcccg ttcgaccagg accgcgacgg cttcgtgatc ggcgaaggag cggcgattct
     2941 cgtgttggaa tcgtccgagc gggcccgccg ccgcggcgcg cgtgcgtatg ccgaggtcgc
     3001 cggcttcggc gcgacgagcg acgcctttca catgatcatt cccagcagcg atcccgcccc
     3061 ggctgcggcg gcgatgcagc tagcgctggc cgatgccgcc atcgagccgc acgagatcga
     3121 ctacatcaac gcccatgcca ccagcacgcc cgtcggcgac aaggccgagg cccgcgccct
     3181 gcactcggtc ttcggctcgc atacggccac gacgcccgtc agctcgacca agagcgtcac
     3241 cggccacctg ctgagcgcgg cagcggcgat cgaggccctg gcctgcctcg tcgcgatccg
     3301 cgagcaggcg attccgccga cgatcaacct ggagcggccc gatcccgagt gcgacctgtg
     3361 ccacgtgccg caccaggcga tcgcccatcc ggtcgagtac gccctgtcca actcgttcgg
     3421 cttcggcggc agcaacacga gcctgattct gcggaaggcg gcgtagctaa atggccgcgc
     3481 tccgctcggc gatttcgtgg tgcaggatcc ggtcgttgtc ggaatagtcg atcgggacat
     3541 cgatcagctg cacgccgctg gcgcggtggc actcttcgag caacggcagc aagtcggccg
     3601 cacgttcgac gcggcggccg cgagcgccgt agctctcggc gtacttcacg aagtccggat
     3661 tgcccacatc gaggccgaaa tcggcgaacc cttgatggac ctgcttccac ttgaccatgc
     3721 catagccgtc gtcgcggacg atgagcacga ccaggttcat cttcagccgc acggccgtct
     3781 ccagctcctg cgagttcatc aggaagccgc cgtcgccgca gacggccatc acctggcggc
     3841 cgggatagac gaggcgggcg gccatggccg acggcaagcc cgcgcccatc gttgccaggg
     3901 cgttgtcgag cagcaccgtg ttgggctggc gggcctggta gttgcgggcg aaccagagtt
     3961 tgtaaatgcc gttgtcgagg gccaggatgc cgtcgtcctg catcgcgcgg cggacgtcgg
     4021 ctacgaggcg ctgcggatag atcggaaacc gcgggtcgtc cgcgccttgc cgcacgtgct
     4081 cttccagcgc ggcgccgatt cgctcaaagc gagagaagtc ccagtggccg ggcttgctca
     4141 gacgttcggt gagctgccag atgctgttgg caatgtcgcc cacgacttcg acctgcggga
     4201 aatagaccgg atcgacctcg gcggtaaaga agttgacgtg gatgacttgc acgccgccgg
     4261 cccgcatgat gaacggcggc ttttcgacca cgtcgtgccc gacgttgatg atcaggtcgg
     4321 ccgcctcgac cgcccggtgg acgaagtcgc cggacgagag cgcggcggtt cccaggaaca
     4381 gcggatgcgt ctcatccacg acgcctttgc ccatttgcgt cgtgacgaat ggaatgccgg
     4441 tggtctcgat gagccgccgc agcatccgcg aagtcgtgcg gcggttggcg gccgcgccga
     4501 ccaggaggag cggatgccgc gattgctcga tgcggctgac tgccgcgcgg atcgccttct
     4561 cttcggccac cggccggcgg acgtaactcg ccgagagcaa cggctcgctc gtttcctcgc
     4621 gggcgacgtc ctcgggcaat tccaggtgcg tcgcgccggg ccgctcctcc tcggccaggc
     4681 ggattgcttc gcggacccgc gagggaatgc tgccgccgct gacgatctgc cgcgtcgatt
     4741 tcgtcagcgg ccgcatcatg tccacgacat cgacgagctg gaattgcccc tgcttgctgg
     4801 tcttgatcgg cttctggccg gtgatcatca gcatcggcat gccgccgagc tgggcatagg
     4861 ccgcggccgt gacgaggttc gtcgcgcccg gtccgagcgt cgagaggcag acgcccgcct
     4921 tgccggtcag ccgcccgtaa gtcgcggcca tgaagccggc cgcctgctcg tggcgggtga
     4981 cgatcaagcg gatgctcgat ctcgacagcg cgtcgaggag ctccaggttc tcttcgcccg
     5041 gaatcccgaa gacgtattgg acccgctcgc tttccagggc tttgacgaag agctcggagg
     5101 cgttcatagc ggtcggtcgt cggtggtcgg tctgtcggtc agtcggtggt cggtctgtcg
     5161 gtggtcggtc gatgcttata ccgaccaacc gatcaccgac caccgaccta ccactcacgc
     5221 ccctttgggc tttccccaat gctcgcgata ggtccgccgc acgagggcgt tggcttcgtc
     5281 gtcgccgacg atttgctcgg tctctcggtc gaagttcagc gagcggccca gccgggtggc
     5341 gatgttgccc aggtggcaga gcgacgtcga gagatggccg gtcagcgccg gagcgttcaa
     5401 cttcgcctcg ccgcggactg cctcgcagaa gttgcggacg tgcgacgcgt cgtcctgcgc
     5461 tcccggctgc acgggcacgt ccaccggctt gttgccatcg gcctgcacct ggatcttgcc
     5521 gcggcggctg agatacatct ggcccttcgt gccatagaac tctgcgccgc tgtcggtgtt
     5581 gtgcgggtag ttggtggacc agagccgctg ct
//
LOCUS       DASRJB010000544         7598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2687850, whole genome shotgun sequence.
ACCESSION   DASRJB010000544 DASRJB010000000
VERSION     DASRJB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687850"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFB80_14155"
     CDS             <1..584
                     /locus_tag="VFB80_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HZN34967.1"
                     /translation="VSLESKLANVEGTGAYTIRDLLKNALRMRPDRIIVGECRGPEAL
                     DMLQAMNTGHDGSLTTVHANSAAEVLQRLEVLVLMAADLPVPAIHRQIDSAIDLVVHI
                     SRVSGGKRAVTQISEMSGVDENGQVKIRDIFNLRGESEELQPTGYMPTFVDQLVAKNL
                     LDARFLYSKRAPEAPATNGRGRSAPSPAGRGPA"
     gene            662..1513
                     /locus_tag="VFB80_14160"
     CDS             662..1513
                     /locus_tag="VFB80_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN34968.1"
                     /translation="MNPTLLPLLSSLLLGSAAALGAYGGRNWLGAGFAWIERDVGDKL
                     RALRIAPRSLRRWLIAWAVTIGSVLVVLGLALQSIVPALLICALLAALPWYVVRRLAE
                     ERRRKIEDQLADSMVSFSNGVRAGLSIAQALDILAEQSPRPVRDEFRQITSEFSLGRP
                     LEQTLVEAKERLKSENFSLFAAALLASRRSGGKLNETVERIARSVLEMQRLERKVQSE
                     TAQARKSAVYMAIAPAAILLVYYFVDPENTTALFTTFFGQVLISIAVVLNVVAYVWAR
                     MILSPDI"
     gene            1555..2457
                     /locus_tag="VFB80_14165"
     CDS             1555..2457
                     /locus_tag="VFB80_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN34969.1"
                     /translation="MQLALLASLLIGLAAACLAWWFVRALADVDDNDKEWRYDRVRIA
                     ELKRHDGLYRWLYPLFAPLARINRGLFRGSLPEIQRELAAAGLPRFWLAEEYLARCEL
                     LGLALMPAYAMSCWMMFGPVGIVLAVVFSALTAWLLRRNLRSRARYRLVLIKRRLPFF
                     LDMLTLLMEAGSTLLQALEEAVHEFRGQPVAQEFGRVLADIRMGRTRAESLLAMRDRL
                     EDSEVGGVLGSILQGEHLGTPLAQLFRMQADVLRVKRTQRAETIAGEAGVQMLLPGIL
                     VMAATVLVILGPFALNYLTFGFNL"
     gene            2595..3362
                     /locus_tag="VFB80_14170"
     CDS             2595..3362
                     /locus_tag="VFB80_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34970.1"
                     /translation="MAASSAFAGQMIACPNCRGEFLMTPPGMMPPPAAPLPPPSGSYA
                     PPPVAANPAPPAAPQAVAVAAPPAARPMPAPAPAVVPPPATVPAQPPPNTARFKTAAT
                     QAPAIAPAADGKLPTLQLADASAPAAKGGSGEKGVPLWAAAMAVVASTVISAILLLGD
                     LEGTQSTGSRRTFARQKLTEHYATGSAEPRAYQVLLREAQQAHTRGDFATERKKYRDV
                     LALLRSEDRKKKFDWLTGAESSDQDLENQLSILLAND"
     gene            3456..6734
                     /locus_tag="VFB80_14175"
     CDS             3456..6734
                     /locus_tag="VFB80_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN34971.1"
                     /translation="MQPHDRYEILGLIGNGDFAAVYRARDRELGRDVAIKQIHGQYLS
                     DPRRLERFWREAQLLASLQHPHVLTIYDVVRPRGWLVLELMHGTIQDAARGQPLDLEM
                     LRSALIGSLSALAMLHGNQVLHGDIKPSNLLLDRQGRVKLGDFGLARRVASDQGSYLK
                     GATRYMAPELVAPQFGPVCPASDLYSLGFTAYELLCGPQQFENLFPGLDAFGRDRQIA
                     WMMWHAAPDRRLPPVGSVLDGVPTDVARVIDRLTTKDQSRRYRSADEALADLSAQGAV
                     AVPAEDDGAAEAGRKKFQKRLVAGLALCASLLICTLILVMPGKKPPPPAPPPPVAIRG
                     AVRTLLPERQTVIIEQAGDLGPKEIVVRSNDRVLLNDKVILFSELREADQVTIQTLKD
                     DQGRPLLEIAATRPQASQGTITRVAADIGELVLALAAGGELKLNVGQETPIELNGESR
                     KKGQPLALADLAAGDRVAVQHYRPAEREEAMRIVAQRIVPGKGVVRAIDLKKGQISIA
                     DGPEDGSPVTIWRFAPQPEITLNGRRVLNSRLLTPADLQVGDQVSFQRDVLLVSVAAQ
                     RQFADSGTVRAIRYDVRSLTAAVAGGAERTFVLAPQCTVTLGGQSAAFDDLRRGDTFH
                     VTFDEPEAVSPAVTSIAAQRPRDASKWVVAIAGAAYDDSSVPAMPTAAAAVARLQAAL
                     AARYAVPPEQVLVLSDPSRVRLEQGLSESLAKTASAGQLWVVVAGRVLSDGKNPPLVA
                     PRDFARARADATGVPLTTILSAVEGIAVKDKLVLLELHSVGGLSGQKSAAALVDSVRG
                     TRSKPLLRTTPVLALDSVPATDAPAALVEALATGALGAADPNRDNLITLAELTDYLKS
                     SAASSVRLILPETTPPRLTDDAKEAIRRVAAAVAQQKIDSTDAMFLISAADRLAPKQP
                     EPKLVGGILLLKARQHNDAIDHLAQVVAQYPRTPLAWEVSAWARFEKLNFTGGISDLT
                     QMIRNLPEGTLPDADRRAIPWVGRLREFAANAASVDRRPPPAAIEALDAAVATRSEDV
                     QMLYRQGREAVSQIIQDFDRRVLAADPNEQIKIKLDRVALRHYTAFSLEAASQQALAT
                     LDVE"
     gene            7405..>7598
                     /locus_tag="VFB80_14180"
     CDS             7405..>7598
                     /locus_tag="VFB80_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HZN34972.1"
                     /translation="MNALPSPTIFMQEAIRLAIDGARQGRGGPFGAVVVREGQIVGRG
                     CNCVTSTNDPTAHAEVIAIR"
BASE COUNT         1264 a         2576 c         2556 g         1202 t
ORIGIN      
        1 tcgtcagtct cgaaagcaaa ctggccaacg tcgaagggac cggggcttat acgatccgcg
       61 atctgctgaa gaacgccctg cggatgcggc ccgaccggat catcgtcggc gagtgccgcg
      121 gacccgaggc cctggacatg ctccaggcga tgaacaccgg ccatgacggc tcgctgacca
      181 cggtgcacgc caacagcgcg gccgaggtgc tgcagcggct ggaggtgctc gtgctgatgg
      241 cggccgacct gcccgtgccg gcgatccatc ggcagatcga ctcggcgatc gacctggtcg
      301 tgcacatcag ccgggtttcg ggcggcaagc gggcggtgac gcagatttcc gagatgagcg
      361 gcgtcgatga aaacggccaa gtgaagattc gcgacatctt caacctgcgg ggcgagagcg
      421 aagaactgca gccgacgggc tacatgccca cgttcgtcga tcagctcgtg gccaagaacc
      481 tcctcgacgc gcggtttttg tattccaagc gagcgccgga agcgccggcg acaaacggac
      541 gcggccgctc cgctccctct cccgctggga gagggccggc gtgagggttt tcaagagcag
      601 ggcgatcacg caacgtagag acctcaccct aaccctttcc atagggagcg gggaccgaaa
      661 catgaacccc acgctcctgc ccctcctcag cagcttgctc ctcggctcgg ccgcggcgct
      721 gggggcgtat ggcggccgca attggctcgg cgcgggcttc gcctggatcg agcgcgacgt
      781 gggcgacaag ctgcgggcct tgcggattgc gccgaggagc ctccgccgct ggctgatcgc
      841 ctgggccgtt acgatcggca gcgtgctggt ggtgcttggc ctggccttgc agagcatcgt
      901 gccggcgctg cttatctgcg cgctcttggc ggcgctgccc tggtacgtcg tccgccggct
      961 ggccgaagag cggcggcgga agatcgaaga tcagctcgcc gattcgatgg tctcgttcag
     1021 caacggcgtg cgggccggcc tgtcgatcgc ccaggccctc gacatcctgg ccgaacagtc
     1081 gccgcggccg gtccgcgacg aattccgcca gatcaccagc gagttcagcc tcggccgccc
     1141 gctcgagcag acgctggtcg aggccaagga gcgcctcaag agcgagaatt tctcgctctt
     1201 cgccgcggcc ctgctggcca gccgtcgcag cggcggcaag ctcaacgaaa cggtcgagcg
     1261 gatcgcccgc tcggtgctgg agatgcagcg gctggagcgt aaggtccaga gcgaaacggc
     1321 ccaggcccgc aagtcggccg tctacatggc gatcgccccg gccgccatcc tgctggtcta
     1381 ttatttcgtc gatcccgaga acaccaccgc cctgttcacg acgttcttcg gccaggtgct
     1441 gatctcgatc gccgtcgtgc tgaacgtcgt cgcctatgtt tgggcgcgga tgatcttgag
     1501 tcccgacata tagaagaacc cagaaccaaa gaactgaaga tccaaagaac cgaaatgcag
     1561 ctcgccctcc tagccagtct cctcatcggc ctggcggcgg cctgccttgc ctggtggttc
     1621 gtccgcgcgc tcgccgacgt ggacgacaac gacaaagagt ggcgctacga ccgcgtccgg
     1681 atcgcggagc tcaagcggca cgacggcctc taccgctggc tctatccgct gttcgcgccg
     1741 ctggcccgga tcaatcgcgg cctgttccgc ggctcgctgc cggagatcca gcgcgaattg
     1801 gccgccgctg gcctgccgcg gttctggctg gccgaggaat acctggcccg ttgcgaactg
     1861 ctcggcctgg cgttgatgcc ggcctatgcg atgtcttgct ggatgatgtt cggaccggtg
     1921 ggaatcgtcc tggcggtcgt cttctccgcc ctgactgcct ggctcctgcg gcgcaacctc
     1981 cgcagtcggg ctcgctaccg cctggtgctg atcaaacgcc ggctgccgtt ctttctcgac
     2041 atgctgacgc tgctcatgga ggccggctca acgctgctgc aagccctgga agaagcggtg
     2101 cacgagttcc gcgggcagcc cgtggcccag gagtttggcc gcgtgctggc cgacatccgc
     2161 atggggcgga cgcgggccga gtcgctgctg gcgatgcgcg accgcctgga agacagcgaa
     2221 gtcggcggcg tcctgggctc gatcctgcag ggggagcacc tcggcacgcc gctggcccag
     2281 ctcttccgca tgcaggccga cgtgctgcgg gtcaagcgga cgcagcgggc cgaaacgatc
     2341 gccggcgaag cgggcgtgca gatgctgctg ccgggcatcc tggtgatggc cgctacggtg
     2401 ctcgtgatcc tggggccgtt cgcgctgaat tacctgacgt ttgggtttaa tctgtagggt
     2461 aggtcagtgg tcagtagtca gtggccagta ggaccaggtt gagcgctcga tgactccaac
     2521 tgacaactga ccactgccaa ctgacaacca tgtccctgcg cgtagcgatt cactgtccct
     2581 tctgcaagcg gcccatggcg gcttcgtcgg catttgcggg gcaaatgatc gcctgcccga
     2641 attgccgcgg cgagtttttg atgacgccgc cagggatgat gccgcccccc gctgcgccgc
     2701 ttccaccgcc ctccggcagt tatgcaccgc cgccggttgc ggccaatcca gcaccgcccg
     2761 ccgcgccaca ggccgtcgcg gtcgcggcgc cgcccgccgc gcggccgatg ccggcgccgg
     2821 cccctgccgt cgttccgccg ccggcgaccg tgcccgctca gccgccgccg aatacggccc
     2881 gcttcaagac ggcggcgaca caggcgcctg caatcgcgcc agcggccgac ggcaagctgc
     2941 cgacgctgca actggccgac gcctccgctc cggcagccaa gggcggatcg ggcgagaagg
     3001 gagtgcccct ctgggcggcg gcgatggccg tcgtggcgag caccgtcatc tcggcgatcc
     3061 tgctcctggg cgacttggaa gggacgcaga gcactggctc gcgccgcacc tttgcccgcc
     3121 agaagctgac cgagcactac gctaccggtt cggccgaacc gcgggcctac caggtgctgc
     3181 tgcgcgaagc ccagcaggcc catacccgcg gcgatttcgc caccgaacgc aaaaaatacc
     3241 gcgacgtgct ggcgctgctg cgctccgaag atcgcaagaa gaaattcgac tggctcaccg
     3301 gcgcggaatc cagcgaccag gatttggaga accagctttc gatcctgctg gccaatgatt
     3361 agaaaatgat ccgtcgttta gccgcagcca gaggcccagg cgtattccgc tggccgccgt
     3421 cgcctccggc tccgggttag acgatattgg tgcttatgca gccgcacgac cgctatgaaa
     3481 tcctgggcct gatcggcaac ggcgatttcg ccgcggtcta tcgcgcgcgc gaccgggagc
     3541 tcggccgcga cgtggcgatc aagcagatcc acgggcagta cctcagcgac ccgcggcggc
     3601 tggagcggtt ctggcgcgag gcccagctgc tggcctcgct ccagcatccc cacgtgctca
     3661 caatctacga cgtcgtgcgg ccccgcggct ggctggtgct cgagctgatg cacgggacga
     3721 ttcaggatgc cgcccgcggg cagccgctgg atctggaaat gctccgctcg gcgctgatcg
     3781 gctcgctttc ggcgctagcg atgctgcacg gcaaccaggt gctgcacggc gacatcaagc
     3841 ccagcaacct gctgctcgac cggcaggggc gtgtgaagct cggcgacttc ggcttggccc
     3901 gccgcgtggc cagcgaccag gggagctacc tcaaaggggc cacgcggtac atggccccgg
     3961 agctggtcgc gccgcagttc gggccggtct gccccgcgag cgacctgtac tcgctcggct
     4021 tcacggccta cgagctgctc tgcgggccgc agcaattcga gaacctcttt cccggcctgg
     4081 acgcctttgg ccgcgaccgc cagatcgcct ggatgatgtg gcacgcggcc cccgaccggc
     4141 gcttgcctcc agtcgggtcc gtgctcgatg gcgtgccgac cgacgtggcc cgcgtgatcg
     4201 acaggctgac gaccaaggac cagtcgcgtc gttaccgctc ggccgacgag gccctggccg
     4261 acctgagtgc acaaggggca gtggccgtcc cggcggaaga cgacggcgcg gccgaggcag
     4321 ggcggaagaa attccagaag cgtctcgtgg ccggcctggc gctctgcgcg agcctgctta
     4381 tctgcacgct gatcctggtg atgccgggca aaaagccgcc gcctcccgcg ccgccgccgc
     4441 cggtggcgat ccgtggcgcg gtgcgcacgc tgctgcccga gcggcagacc gtaatcatcg
     4501 agcaggcggg cgatctggga ccgaaagaga tcgtcgttcg ctccaatgac cgcgtgctgc
     4561 ttaacgacaa ggtgatcctg ttcagcgagc tgcgcgaggc ggaccaggtt acgatccaga
     4621 cgctcaagga cgaccagggc cggccgctgc tggagatcgc tgccactcgc ccgcaggcca
     4681 gtcagggcac gatcacgcgg gtggcggccg acatcggcga attggttctg gcgctggcgg
     4741 ccggcggtga gctgaaactg aacgtcggcc aagagacgcc gatcgagctc aacggcgaga
     4801 gccgaaagaa aggccagccc ctggcgctgg ccgacctggc ggcgggcgac cgcgtcgcgg
     4861 tgcagcacta tcgccccgcc gagcgcgaag aggcgatgcg gatcgtcgcc cagcggatcg
     4921 tccctggaaa gggcgtcgtg cgggcgatcg acctcaagaa gggccagatt tcgattgccg
     4981 acggtccgga ggacggctca ccggtcacaa tctggagatt cgccccgcag ccggagatca
     5041 cgctcaacgg ccgccgcgta ctgaacagcc ggctgctcac gccggccgac ctgcaggtcg
     5101 gcgatcaggt ttcgttccag cgcgacgtgc tgctggtgag cgtcgccgcc cagcggcagt
     5161 tcgccgatag cggcacggtc cgcgccatcc gctacgacgt tcgctccctg acggcggcgg
     5221 tcgcgggcgg cgcggagcgg acgttcgtgc tggctccgca gtgcaccgtc acgctcggcg
     5281 ggcagagtgc cgcgttcgac gacctccgcc gcggcgatac ctttcacgtc acatttgacg
     5341 agccggaagc cgtcagtcct gccgtgacca gcatcgcggc ccagcgcccc cgcgatgcca
     5401 gcaaatgggt cgtcgcgatt gccggcgcgg cctacgacga ctcttccgtg ccggcgatgc
     5461 ccacggccgc ggccgcggtc gccaggctgc aggcggcact ggcggcgcgg tacgcagtcc
     5521 cgcccgagca agtcctcgtc ctcagcgacc ccagccgcgt gcgcctggag caggggctgt
     5581 ccgaatcgct ggccaagacg gccagcgccg ggcagttgtg ggtcgtcgtg gccggccgcg
     5641 tgctgagcga cggcaagaat ccgccgctgg tagcgccgcg cgatttcgcc cgcgctcggg
     5701 ccgacgcgac cggcgtgccg ctgacgacga tcctctccgc cgtcgaaggc atcgccgtca
     5761 aggacaagct cgtgctgctg gagttgcact cggtcggcgg cctgtcggga caaaagtcgg
     5821 ctgcggctct ggtcgatagc gtgcgcggca cgcgctcgaa gccgctgttg cgcacgacgc
     5881 cggtcctggc gctggactcc gtcccggcca ccgatgcgcc ggcggcgcta gtcgaagcac
     5941 tggccaccgg cgccctagga gcggccgatc ccaaccgcga caacctcatc acgctcgccg
     6001 aattgaccga ctacctcaaa tcgtcggcag ccagcagcgt gcggctgatc ctgcccgaaa
     6061 cgacgccgcc ccgcctgacc gacgacgcta aggaagcgat tcgccgcgtg gccgcggccg
     6121 tcgcccagca gaagatcgac tccacggacg ccatgttcct gatcagtgcc gccgaccggc
     6181 tcgcccccaa gcagccggag cccaagctcg tcggcggaat cctgctgctc aaggcccgcc
     6241 agcacaacga cgccatcgac cacctggcgc aggtcgtggc ccagtatccg cggacgccgc
     6301 tggcctggga ggtgagcgct tgggcccggt tcgagaagct gaacttcacc ggcggcatca
     6361 gcgacctgac gcagatgatc cgcaacctgc ccgaaggaac gcttcccgac gcggaccgcc
     6421 gggcgattcc ctgggtcggc cggctgcggg agttcgcggc gaatgccgcc tcggtcgatc
     6481 gccgtccgcc gcctgccgcg atcgaagcgc tcgacgccgc cgtggcgacc aggagcgaag
     6541 acgtgcagat gctttatcgg caggggcgag aagcggtaag ccaaatcatc caggacttcg
     6601 accgccgcgt gctggcggcc gaccccaacg aacagatcaa gatcaagctc gaccgcgttg
     6661 ccctgcggca ctacaccgct ttttcgctcg aagccgccag ccagcaggcc ctggccacgc
     6721 tggacgtcga gtgacgtcaa accgtccggg cttgcggttt gccgccgaat tgtgttctgg
     6781 atagaataaa tgaactctcg tgcgcatcag agagatgggc gacggctgtt gatccttggc
     6841 aattcggcta ggaaacgtgg gcgcgcataa tgcgcatatt cgccgagcgc gcattttctc
     6901 cacaagtgat ttgcgcatca gcacttgcgg cgaaaacatc gtcccacaga aaaagtccga
     6961 gtatgcgcgc atgcgaccag tgacccggtc tggtgctaat ccgcccgcac cgcagagggt
     7021 gctggcgacc cactcgtgct ggggggccgc gcttcgaggc ctgcaccgcc gttggattgg
     7081 acttgcggtg cgggaaggcc agccgggtag aggtcggccg gaatcaggcg ggcgttgcac
     7141 tgcgggcagt gccagaaggg ccagacgatg cgcatacgcg cacgccgagc gcacgccggg
     7201 cagaggtaaa cgcgcggcgt cagggcgtgc gtgggatcga tgcgatggcg gtccatgagg
     7261 ctccgccgag ggaaccagtt gggacagccc ctcctccgtc caccctttca gtcgtgatgg
     7321 agtggaaaga gcgggcaaaa acagccggaa aaatgcacga cggtaccgct cgcgaagttt
     7381 tgctaagctg ggtggacttt gacgatgaac gcactgccgt ccccgacgat cttcatgcag
     7441 gaggccatcc gcctggcgat cgacggcgcc cggcagggac gcggcgggcc gtttggagcc
     7501 gtcgtcgtgc gagaggggca gatcgtcggt cgcggttgca attgcgtcac ctcaaccaat
     7561 gatccgacgg cccacgccga ggtcatagcg attcgcga
//
LOCUS       DASRJB010000545         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_714184, whole genome shotgun sequence.
ACCESSION   DASRJB010000545 DASRJB010000000
VERSION     DASRJB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714184"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFB80_14185"
     CDS             complement(<1..612)
                     /locus_tag="VFB80_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HZN34973.1"
                     /translation="MPDISDGETVEFKGSAAQPYLLKNVGGVYSCTCPAWRNQSVAIE
                     QRTCKHLRKYRGEEAEKARLGSALPQKPAAKKTADGEEEEGAAGPPILLAQTWANDAD
                     LAGWWMSEKLDGVRAYWDGKQFLSRQGNLYHAPDWFAEQLPPVPLDGELWLARKSFQR
                     TVSIVRRQDKSEHWREISYVIFDAPGVESSFEERQEYLREALAR"
     gene            complement(628..1335)
                     /locus_tag="VFB80_14190"
     CDS             complement(628..1335)
                     /locus_tag="VFB80_14190"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN34974.1"
                     /translation="MSKPPLKILYEDNHLLVVEKPAGLPTQGVAEGTPSVVTRAKAYL
                     KQKYRKPGNVYLGVVSRLDAFVSGVLVLARTSKAATRLTEQFRSSTVSKVYQALVERP
                     PDPPAGELTHWLLKDDKERRIEVVPPRTKNAQHARLSYRTLNKHRRGTLVEIELHTGR
                     KHQIRVQLAEIGCPIVGDRKYGSRQPLADMAIALHAVRLTIEHPTTRQPLTVESPLPE
                     NWNTNPKRQRGNTNPKR"
     gene            1430..2017
                     /locus_tag="VFB80_14195"
     CDS             1430..2017
                     /locus_tag="VFB80_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HZN34975.1"
                     /translation="MPRLPLILASSSPRRRQLLQQAGYTFTVAPPHESAEDEPRPGET
                     PRELVARLARQKASDVARGIERGLVIGCDTVAESGGQILGKPADRGDARRMLAALRGQ
                     EHRVYSGLCLWRRPDDRMWQEVEITTLVMERLTDAELENYLATGDWQGKAGAFGYQDG
                     LDWVRVVSGSESNVVGLPLELLERMIANVDREQVS"
     gene            2025..2735
                     /locus_tag="VFB80_14200"
     CDS             2025..2735
                     /locus_tag="VFB80_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZN34976.1"
                     /translation="MISSDDNSPAPLAAAEPVRLFPLPNLVMFPHVVQPLHIFEPRYC
                     DMLEAALARDKLIAMVLLEPGWESDYEGRPALSPVACLGKVIAHERLPTGRHNILLRG
                     LKRAAIRRELPPAAAFRQAEVDLLDDFYPASSAPQRAQVQRDLLALARRLLPDSDALH
                     EQLDELLASQASLGMLTDIFAFTLGFPTAVKQRLLAEWNVDRRAKLLSDHLSRLAQRV
                     PPVESSDERPYPPRFSLN"
BASE COUNT          435 a          958 c          913 g          525 t
ORIGIN      
        1 gcgggcgagc gcttcgcgca gatattcctg ccgctcctcg aacgaggatt ccacacccgg
       61 ggcgtcgaag atcacgtacg agatttcgcg ccaatgctca ctcttgtcct gccggcggac
      121 gatgctcact gtccgctgaa acgacttgcg ggccagccac aactctccgt cgagcggcac
      181 cgggggcagc tgctcggcga accagtcggg cgcgtggtac aggttgccct gccgcgagag
      241 gaattgcttg ccgtcccaat aggcccgcac gccgtcgagc ttctcgctca tccaccagcc
      301 ggccaggtcg gcgtcgttcg cccaggtctg ggccagcagg atcggcgggc cggcggcgcc
      361 ttcctcttcc tcgccgtccg ccgttttctt cgccgccggc ttttgcggca gggccgaccc
      421 cagccgggcc ttctcggcct cttcaccgcg atactttcgc aggtgcttgc aggtccgctg
      481 ctcgatcgcc accgactggt tccgccaggc cgggcacgta cacgaataga cgccgcccac
      541 gtttttcagc aggtacggct gcgccgccga gcctttgaac tcaaccgtct cgccgtcgga
      601 aatgtctggc atgagcggat tgcctcgcta acgtttgggg ttggtgttcc ctcgctggcg
      661 cttcgggtta gtattccaat tctccggcag cggcgattcg accgtcagcg gctgccgcgt
      721 tgtcgggtgc tcgatcgtca ggcggacggc gtgcagggcg atcgccatgt cggccagcgg
      781 ctgccggctg ccgtacttcc ggtcgccgac gatcgggcag ccgatctcgg ccagctgcac
      841 gcgaatctgg tgcttgcggc cggtgtggag ctcgatttcg accagcgtgc cgcggcggtg
      901 cttgttcagg gtccggtagc tcaagcgggc gtgctgggcg tttttcgtcc gcggcggcac
      961 gacctcgatc cgccgctctt tgtcgtcctt cagcagccag tgcgtgagct ccccagcggg
     1021 cggatcgggc ggccgctcga ccagcgcttg atagacctta ctgaccgtac tcgagcggaa
     1081 ttgctccgtc agccgggtcg cggccttgct ggtgcgggcc agcaccagga cgccgctcac
     1141 gaaggcatcg agccggctga cgacgcccag atagacgttg cccggcttgc ggtacttctg
     1201 cttgagatac gccttggccc gcgtcaccac gctcggcgtc ccctcggcca cgccctgcgt
     1261 cggcaggccc gccggtttct cgaccaccag caggtggttg tcttcgtaga ggattttcag
     1321 cggcggcttg gacatgcaaa aaaccaaccc gacgcgtacg cgaggggctt cttcagggcg
     1381 ggctgcactc aagaaatccc tcgctcacgc ttggggttaa tatatcccaa tgccacgttt
     1441 gccattgatt ctcgccagca gctcgccgcg gcggcggcaa ttgctgcagc aggccggtta
     1501 cacgtttacc gtggcgcctc cgcacgaatc tgctgaagat gaacctcgcc ctggggagac
     1561 gccacgcgag ctcgtcgccc ggctcgcccg gcaaaaagcc agcgacgtgg cccgcggcat
     1621 cgagcggggc ctcgtcatcg gctgcgatac ggtcgccgag agcggcgggc agattctcgg
     1681 caagccggcc gaccgcggcg acgcccgccg gatgctcgcc gcgctccgcg gccaggagca
     1741 ccgcgtctat agcggcctct gcttgtggcg gcggcccgac gatcggatgt ggcaggaggt
     1801 cgaaatcacg acgctcgtaa tggaaaggct taccgatgca gaactcgaaa actacctggc
     1861 aaccggcgac tggcagggca aggccggggc cttcggctat caggacgggc tcgattgggt
     1921 ccgcgtcgtc tccggcagcg agtcgaacgt cgttggcttg cctttggaac tgctggaacg
     1981 gatgattgcc aatgtagatc gcgagcaagt ctcgtagccg ccacatgatt tcgtcggacg
     2041 ataactcgcc tgcaccactg gcagcggcgg agcccgtgcg gctctttccg ctgccgaacc
     2101 tggtgatgtt tccgcacgtc gtgcagccgc tgcacatttt tgagccgcgc tattgcgaca
     2161 tgctggaggc agccctggcc agggacaagc tgatcgcgat ggtcctgctg gagcccggtt
     2221 gggagagcga ctacgaaggc cggccggcgc tgtcgccggt ggcctgtctg ggcaaggtca
     2281 ttgcccacga gcgcttgccg accgggcggc ataacatcct cctgcgaggc ctcaagcggg
     2341 cggcaatccg gcgggagctg ccgccggcgg cggcgttccg ccaggccgag gtcgatctgc
     2401 tggacgattt ctatcctgcc tccagcgccc cgcaacgagc ccaagtgcag cgtgacctgc
     2461 tcgcgctggc ccgccggctg ttgcccgatt cggacgccct gcacgagcag ctcgacgagc
     2521 ttctggcgag ccaggcctcc ctcggcatgc tcaccgacat cttcgcgttc acgctcggct
     2581 ttcccaccgc ggtgaagcag cggctgctgg ccgaatggaa cgtcgatcgc cgggccaagc
     2641 tgctgagcga ccacctgagc cgcctggccc agcgcgtgcc gcccgtcgaa tcgtccgacg
     2701 agcgacccta tccgccgcgg ttcagcttga attgatcgac gctgccgacc gatcgcggat
     2761 tcgacatgca cgcacttaga atggggacat ggaacggttc tagactccaa tcgtcgcatt
     2821 gccgcatcac c
//
LOCUS       DASRJB010000546         5701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3815628, whole genome shotgun sequence.
ACCESSION   DASRJB010000546 DASRJB010000000
VERSION     DASRJB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815628"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..3238
                     /locus_tag="VFB80_14205"
     CDS             41..3238
                     /locus_tag="VFB80_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN34977.1"
                     /translation="MLDALSNRRAGLALLLAAVAAQAQAGDKLQYNRDIRPILAENCF
                     ACHGPDSAARKSELRLDRREAAVEYGAIVPGKTDESLIVERITSADPMMVMPPPATKK
                     RLTAAQQARLKQWIAEGAEYQPHWSLIAPSRPAPPAVKNSAWVRNPIDAFVLAKLEAA
                     GLSPAPEADRRTLARRLSLDLTGLPPDPELVESFVNDKEANAYENVVEKLMSSPHWGE
                     HRGRQWLDVARYADTHGIHFDNYREIWAYRDWVINAFNHNRPFDQFVVEQLAGDLLPG
                     ATLDQKIATGFVRCNITTNEGGIIDEEYKVLYARDRTETFGLVFMGMTVGCAVCHDHK
                     FDPVSQKDFYSLTAFFDNTTQAVKDGNIKDTPPVVVVPAAADRQRWDELQAELVEVRF
                     QLEARKRDAQPAFDEWLASVKAADLGQYVPQEGLALQATLDEGKGKSLTLVVNGAERE
                     LAREEAINWAPGRDPKQKVLSLATTPAIEIADAGDFEADHGFAVSVWVKLGRRNQTGA
                     ILSRMDNASDYRGWDLWLEGDKVGMHVVDKWPDNALKVVSQTPLKANEWTHVCVTYDG
                     SRKAAGVQVYYDGKLQPANVAADKLAGSIKTAVPFKIGQRSTDQKLQFMTVADLRLFG
                     RGLSPQEAEQLAAADQLEAIVAKKAEDRTPDEKAKALDWWLRSLDEPSRKLDARLKAV
                     AADEVAIQGRGTVAHVMNEQAGEPMAHILYRGEYDQKRDQVKATTPAALPPLGEFAPR
                     NRLGLAQWLLTPEHPLTTRVTVNRFWQELFGTGIVRTAGDFGVAGELPSHPELLDWLA
                     VEFRDPSDGSRPWDVKRFFKLLVTSNTYRQMAQVTPEKLAKDADNRLLSRGARYRMDA
                     EMVRDYALAASGLLVRKLGGPSVRPYQPDGVWEAVAMIGSNTRDYKQDSGENLYRRSM
                     YTFWKRAAPPASMEILNAPNRETCAVRRERTDTPLQALVTLNDPQFVEAARHLAQNTM
                     KASGGRQPPEADDARLDYLARRLLARTLRESEHAIVKSSLADLLAHYQANPEDAKQLI
                     AVGESRADPALDPAQLAAWTMLANELMNLDEVLNK"
     gene            3248..3457
                     /locus_tag="VFB80_14210"
     CDS             3248..3457
                     /locus_tag="VFB80_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34978.1"
                     /translation="MNVLHACEAARILPPVSDNCLRPPALLSAEAEDGESVEWIWTHF
                     AGGHSAVTGYRIVPRWPALLCGGSQ"
     gene            3454..4038
                     /locus_tag="VFB80_14215"
     CDS             3454..4038
                     /locus_tag="VFB80_14215"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HZN34979.1"
                     /translation="MTKPKSTLIIDPHELERGIFVVRGQRVMLDAHLAKLYGVPTKRL
                     NEQVQRNADRFPADFAYQLTQQEFMELKSQIATSKTVRGGTQKLPWVFTEHGVAMLSS
                     VLRSPRAVQVNIGIMRAFVRLRRLMATPGELVQHLNELAKTVQLHDGQIAAITDVLRR
                     MMEPPPGPPKRKIGFRPEAKSTSHSNGQPTGDEQ"
     gene            4035..5486
                     /locus_tag="VFB80_14220"
     CDS             4035..5486
                     /locus_tag="VFB80_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN34980.1"
                     /translation="MNPFTEFTQNLTRRHFFQQGSHAVGWAALASLMGSEAGAAEAKQ
                     PAKSLQHFAGHAKHVIYLHMVGGPPQMDLWDYKPVMQEWYDKDLPDSIRRGQRLTTMT
                     SGQARFPIAPSKYKFAQQGQCGMWVSELLPWKSKMVDEMCFIRSMNTEAINHEPAITY
                     IQTGNQVTGRPCLGAWASYGLGSLNDNLPTFVVLVAKPTNTEQVQAISARLWQAGYLP
                     GQHAGCSFRSAGDPILYINNPAGVPSEVRRKTLDGLKALNELNYQQIGDPETRTRIEQ
                     YELAFRMQSSVPELTDLSKESEATLNLYGDAVKKPGSFANTALLCRRLVERGVRFVQV
                     YHNNWDTHSNVAGRLPDQCRDVDQPCYGLVQDLKQRGLLDQTLIIWGGEFGRTIYSQG
                     GLSKENYGRDHHPRCFTIWMCGGGIQGGVIHGETDDFSYNIVKDPVHLRDWHATILHL
                     LGFDHERFSVRHLGLDQRLTGVEPAKVIQPLLA"
BASE COUNT         1102 a         1844 c         1857 g          898 t
ORIGIN      
        1 gcgggcgagt tcgttgccgc gcgacatcca ggaagaaact atgctcgacg ccctttcgaa
       61 tcggcgcgct ggattggctt tgctgctggc ggccgtggcc gctcaggcgc aggccggcga
      121 caagcttcaa tacaaccgcg atatccggcc gatcctggcg gaaaactgct ttgcctgtca
      181 cgggccggac agtgctgccc gcaagtcgga gctgcggctg gaccgtcgcg aggcagccgt
      241 cgagtacggc gcgatcgtgc cgggcaagac cgacgagagc ctgatcgtgg agcggatcac
      301 gtcggccgat ccaatgatgg tcatgccgcc gccagcgacg aagaagaggc tgacggccgc
      361 gcagcaggca aggctcaagc agtggatcgc cgaaggggcc gagtatcagc cgcattggtc
      421 gctgatcgcg ccgtcgcggc ccgctccgcc ggcggtgaag aactcggcgt gggtgcgaaa
      481 cccgatcgat gcctttgtgc tggcgaagct ggaagcggcg ggcctctcgc ccgcgccgga
      541 ggccgaccgc cggacgctcg cccgccggct gagcctcgat ctgacgggcc tgccgcccga
      601 tccggagctg gtcgaatcgt tcgtcaacga caaggaggcg aacgcctacg agaacgtcgt
      661 cgagaagctg atgtcgtcgc cgcactgggg cgagcatcgc ggccgccagt ggctcgacgt
      721 ggcccgctat gccgatacgc acggcatcca tttcgacaac tatcgcgaga tttgggccta
      781 tcgcgactgg gtgatcaacg ccttcaacca caatcggccg ttcgaccagt tcgtggtgga
      841 gcagctggcc ggcgacctgc tgccgggggc gacgctcgac cagaagatcg ccaccggctt
      901 cgtccggtgc aatatcacga cgaacgaagg cgggatcatc gacgaggaat acaaggtgct
      961 gtatgcccgt gaccgcaccg agacgtttgg cctcgtcttc atgggaatga ccgtcggctg
     1021 tgcagtctgc cacgaccaca agttcgatcc ggtgtcgcag aaggacttct attcgctgac
     1081 ggccttcttc gacaacacga cgcaggccgt gaaggacggc aacatcaagg acacgccgcc
     1141 ggtggtcgtg gtgccggcgg ctgccgaccg gcagcggtgg gatgaactgc aagccgagct
     1201 ggtggaagtg cgctttcagc tcgaagcccg caagcgggac gcccagccgg cgttcgacga
     1261 gtggctggcc agcgtgaagg ccgcggacct ggggcagtat gtgccgcagg aaggcctggc
     1321 cctgcaggcg acgctcgacg agggcaaagg caagtcgctg acgcttgtgg tcaacggggc
     1381 cgagcgcgag ctggcgcggg aagaggcgat caactgggcg ccgggccgcg atccgaaaca
     1441 gaaagtgctc tcgctggcga cgacgccggc gattgagatt gccgacgccg gcgatttcga
     1501 ggccgatcac ggctttgcgg tcagcgtctg ggtcaagctc ggccgccgca atcagaccgg
     1561 agcgatcctc tcgcgcatgg acaacgccag cgactatcgc ggctgggatc tttggctcga
     1621 aggcgacaag gtcggcatgc acgtcgtcga taagtggccc gacaatgccc taaaggtcgt
     1681 ctcgcagacg ccgctcaagg ccaacgagtg gacgcacgtc tgcgtcacct acgacggctc
     1741 gcgcaaggcg gcgggcgtcc aggtgtatta cgacggcaag ctccagcctg cgaatgtcgc
     1801 cgccgacaag cttgccggct cgatcaagac cgccgtgccc ttcaagatcg gccagcggag
     1861 caccgaccag aaactccagt tcatgaccgt tgccgacctg cggctgtttg gccgcggact
     1921 ctcgccgcaa gaggccgagc aactggcggc cgccgaccag ctggaggcga tcgtcgccaa
     1981 gaaggccgag gaccggacgc ctgacgagaa agccaaggcg ctggactggt ggctgcggtc
     2041 gctcgacgag cccagccgca agctcgatgc aaggctgaag gcagtcgcgg cggacgaggt
     2101 cgccatccaa ggccgcggca ccgtcgccca cgtcatgaac gagcaggccg gcgagccgat
     2161 ggcccacatt ctctatcgcg gcgagtacga ccagaagcgg gaccaggtga aggccacgac
     2221 gcccgccgcc ctgccgccgc tgggcgagtt cgctccgcgg aaccggctgg ggttggcgca
     2281 atggctgctc acgcccgagc acccgctcac cacgcgcgtg acggtcaatc gcttctggca
     2341 ggaactattc ggcacgggaa tcgtgcggac tgccggcgac ttcggtgtgg cgggcgaatt
     2401 gccctcgcat cccgagctgc tcgactggct ggcggtcgaa ttccgcgatc ccagcgacgg
     2461 cagccggccg tgggacgtca aacggttctt caagctgctg gtgacgagca acacatatcg
     2521 ccaaatggcg caggtcacgc ccgaaaagct ggcgaaggac gccgacaacc ggctgctctc
     2581 gcgcggggcc cgctaccgga tggacgccga gatggtccgc gactacgcgc tggccgccag
     2641 cggcctcctg gtccgcaagc tcggcggacc gagcgtgcgg ccgtaccagc ccgacggcgt
     2701 gtgggaggcc gttgcgatga tcggcagcaa cacccgcgac tacaagcagg acagcggcga
     2761 aaacctctac cgccgcagca tgtacacgtt ctggaagcgg gccgccccgc ccgcctcgat
     2821 ggaaatcctc aatgccccca accgcgagac ctgcgccgtg cgccgcgaac ggaccgacac
     2881 gccgctgcag gcgctcgtga cgctcaacga tccgcagttt gtcgaagccg cgcggcactt
     2941 ggcgcagaac acaatgaagg cgagcggggg gcgtcagccc cctgaggccg acgacgcccg
     3001 cctcgactac ctcgcccgtc gtctgctcgc tcggactctg cgagagtcgg aacacgccat
     3061 cgtcaagtcc tcgcttgccg atctgctcgc ccactatcag gccaatcccg aagacgccaa
     3121 acagctcatc gccgtcggcg aatcgagagc cgatcccgcc ctcgatccgg cgcagctcgc
     3181 ggcgtggacg atgctggcga acgagctgat gaatttggac gaagtgctca acaagtaagg
     3241 tgccaggatg aatgttctgc acgcctgcga agctgctcgc atcctgccgc ccgtcagcga
     3301 caactgcctc cgcccgcctg cgctgcttag cgcggaagcg gaagacggcg agtccgtcga
     3361 gtggatttgg acgcacttcg ccggcggcca cagcgccgtc actggctacc gaatcgtgcc
     3421 gcgatggccg gcgctgcttt gcggaggcag ccaatgacga agccgaaatc cacgctgatc
     3481 atcgatccgc acgaattaga gcggggcatt ttcgtggtcc gtggccaacg ggttatgctc
     3541 gatgcgcacc tcgcgaagtt gtatggggtg ccaaccaagc ggctcaatga gcaggtccag
     3601 cgcaatgctg ataggttccc tgcagatttt gcgtatcagc ttacgcagca agagtttatg
     3661 gaattgaagt cgcaaattgc gacatcaaaa acggtgcgcg gcggcacgca gaagttaccg
     3721 tgggtcttta ccgagcacgg cgtcgcgatg ctctccagcg ttctgcgatc gccgcgggcc
     3781 gtacaggtca acatcggcat catgcgggct ttcgtcaggc tgcggcggct gatggctacg
     3841 cccggcgaac tagtccagca tctgaatgag ctggccaaga cggttcaact tcacgacggg
     3901 caaatcgcgg cgatcactga cgtactgcgg cgaatgatgg aaccgccgcc aggcccaccc
     3961 aaacgcaaga ttggcttccg acccgaagcc aaatcaactt ctcactctaa cggacagccg
     4021 acgggcgacg agcaatgaac ccattcactg aattcactca aaacctcacc cgccggcact
     4081 tctttcagca gggctcgcac gccgtgggct gggcggcgct ggcttcgctg atgggcagcg
     4141 aggcgggcgc ggcggaggca aagcagccgg cgaagagctt gcagcatttt gcgggccacg
     4201 ccaaacacgt gatctatctg cacatggtcg gcggaccgcc ccagatggac ctttgggact
     4261 acaagccggt gatgcaggag tggtacgaca aggacctgcc cgacagcatc cgccgcggcc
     4321 agcggctgac gacgatgacc agcggccagg cgcggttccc gatcgcgccg tcgaagtaca
     4381 agttcgcgca gcagggtcag tgcggcatgt gggtcagcga actgctgccg tggaagtcga
     4441 agatggtgga tgaaatgtgc ttcatccgca gcatgaacac cgaggcgatc aaccacgagc
     4501 cggcgatcac ctacattcag accggcaacc aggttaccgg gcggccgtgt ctgggggcct
     4561 gggcctctta cggcctgggc tcgctgaacg acaatctgcc cacgttcgtc gtcctggtcg
     4621 ccaagccgac gaacaccgag caggtccagg cgatctccgc ccgactgtgg caggcgggct
     4681 atctgccggg gcagcacgcc ggctgctcgt tccgctcggc gggcgatccg attctgtaca
     4741 tcaacaaccc tgccggcgtg ccgagcgagg tccgccgcaa gacgctcgac ggcctgaagg
     4801 ctctcaacga actgaattac cagcagatcg gtgacccgga aacgcgaacg cgcatcgaac
     4861 agtacgagct ggccttccgc atgcagtcaa gcgtgccgga actgaccgac ctctcgaaag
     4921 agtccgaggc gacgctgaat ctgtacggcg acgcggtgaa aaagcccggc agctttgcca
     4981 acacagccct actctgccgc cggctggtcg aacgcggcgt gcggttcgtg caggtctatc
     5041 acaacaactg ggacacgcac agcaacgtgg ccggccgcct gcccgaccag tgccgcgacg
     5101 tggatcagcc ctgctacggc ctggtgcagg atctcaagca gcgcggcctc ctggaccaga
     5161 cgctgatcat ctggggtggc gaattcggcc gtacgatcta ctcgcagggc ggcctctcca
     5221 aggagaatta cggccgcgac catcacccgc gatgtttcac gatctggatg tgcggcggcg
     5281 gcatccaggg cggcgtcatc cacggcgaga ccgacgattt ctcctacaac atcgtcaagg
     5341 accccgtcca cctccgcgac tggcacgcca cgatcctgca cctgctcggc ttcgaccacg
     5401 agcggttcag cgtccgccac ctcggcctcg accagcggct gaccggcgtc gagccggcga
     5461 aggtgatcca gccgctcctc gcttaagcgg ggcgggcctt tccagcctgc catggcagac
     5521 aagaatgtct gccccacagg gacgttatcg ctgattctcg tccttgtcga acaggatgct
     5581 gatcgactgg tcgtggtgaa tccgcttgat ggcttcggcc agaagtggcg cgaccgagag
     5641 caccttgatc ttgggatcat cttctccggc gagatgggaa tcgtgtcggt gacggcgagc
     5701 g
//
LOCUS       DASRJB010000547         8479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2116149, whole genome shotgun sequence.
ACCESSION   DASRJB010000547 DASRJB010000000
VERSION     DASRJB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116149"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2324)
                     /locus_tag="VFB80_14225"
     CDS             complement(<1..2324)
                     /locus_tag="VFB80_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZN34981.1"
                     /translation="MKRISLAGLAFLLLGSIAFGQGVLVVINPPHPVPLPRPIPRPRP
                     TPPPMSYKIKELDYHAKIVDQVAQVQVTQSFVNTGSAAMEVSFVFPIPYDGAIDRMTF
                     MVDGKEYDAKLLAKDEARRIYEGYVRRNQDPALLEWVGYGMFQTSVFPVPAGAERKVS
                     LKFSQLLRKDQKLTDLMIPLSTAKYTSSPVEKLSIHAAIESSQEIKSVYSPTHNVSIQ
                     RPDNKHAVVKFEATNTVPTTDFRLLYDVNDGSLGASLISYRPDSSDEGFFLLLASPEI
                     KATSDERPAKTVIFVVDRSGSMSGKKIEQAKEALKFVLNNLRQGDTFNIVAYDSNVES
                     FKPELQKFDDETRKAALGFVDGIYAGGSTNIDGALSTALSMIKDDSRPNFVFFLTDGL
                     PTAGETNEAKIAANAKQNNKVRTRMINMGVGYDVNSRLLDRLSRDNFGQSGYVRPDEN
                     IEAHVSRLYAKMSAPVLTNVAVKIDIEGAGSESGGVSRVYPKEVYDLFAGEQLVMVGR
                     YKKYGMGKVTISGKVGKDERKHDFPGSFTEKSGDQSFAFVEKLWAMRRIGEIIDEMDL
                     KGKNDELLKELVGLSTKHGILTPYTSFLADENAPVRQLADTRLHLDYARRETDRLSTA
                     DGIAGFSQRSDKNVLQNAERAAQAAAPSFSQPAGEARGAGLGAPGAPTPAASLAARAK
                     SGFQGQAGAAIAGGGLGGGYGSPDGGRVSNYFRDIDSDREVYASSVQNVGKETLYKRG
                     NTWVANNCKDVDPEKDKAKIKEVKRFSDDYFALV"
     gene            2824..4068
                     /locus_tag="VFB80_14230"
     CDS             2824..4068
                     /locus_tag="VFB80_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN34982.1"
                     /translation="MRNTSICTAAVVSCLALASASGQEWPQMLGPNRNGISAETGLLE
                     SWPEGGPKVLWRVPGGVGMSGLAISRLKLLTLVQKDGQQFLTAKNAETGAPLWETPLA
                     SEYENQMGNGPRATPTIAGERVFAYTGEGVLVALNFADGKLLWKHNVPVELKGQPADY
                     GMACSPLVVGQHVIVQAGAPQAAVAAYDAASGKLAWTAGEDGAGYSSPALLDVGGQQQ
                     VVAFTGGSAIGLAPEKGTLLWRYPYETDYQCNIATPLAHRGQVFLSSGENHGCVLLGL
                     RADGEKFVPRVIWESQGSESVLRNEWQTSILLDGHLYGFDNIGSAGPVTHLTCVDVAT
                     GKRAWQQTRFGKGNLIAADGKLFISTMKGELVVVRATPKAYEEIGRLAILGSTRQAPA
                     LAGGRLYLRDDKEIVCLDVRKK"
     gene            4123..5799
                     /locus_tag="VFB80_14235"
     CDS             4123..5799
                     /locus_tag="VFB80_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34983.1"
                     /translation="MPPLVQRRLRAPADDGSALFDPPLSQAPALIERNRAVASKLEKH
                     GISARLRVTARIILLGCAEIRDGVQLEREELQRPIILAGHQPELYHPGVWLKNFALSS
                     LATSVGATSLGLIIDNDTVRSTSIRVPSGGSPPVVEVPFDAPADAIPWEARWVSDPAK
                     FAKFRLEVRGAFGSFLKSPAYKHGLLLDRVWPHALAAADEQREMAKRSLEKLRRGGPH
                     PELLAQAVNLGECLATARHRVERELGLVHREAALGAVTGQPPFLEFAHRLLARHREFH
                     AVHNAALAEYRAVNHIRSQTHPVPDLAQDGDWHEMPLWIWTMDDPRRRRVFVREAASS
                     WELTDRQGLVIDGPAAGQDCDEVYSRLLREGIALRPRALITTMFARLVLSDLFIHGIG
                     GAKYDELTDLIVRRFFGVEPPAFITATATFRLPIERLQISLDDVRRSAARIRDLRWHP
                     ETFARNPLVAGEPSLAEKLAALAAEKREYLAQHDLRRCPPEVFARADALNRAMHALLG
                     PVERALRAEHAELVEQFKRAQVLGSREFSFVLFPSEILSARLLDLCKVSS"
     gene            5870..7279
                     /locus_tag="VFB80_14240"
     CDS             5870..7279
                     /locus_tag="VFB80_14240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN34984.1"
                     /translation="MATRTVSVARRSPSITLTKPRPTVAAPGLRLTPARTGDHGLIHR
                     LLVSVFHGPSAGEFHAQLDEPGYEPADRLVVKDGEQIAAHLRLARQTIRVGEAALPAA
                     RFMDLATAPEYRSRGLATTMLAAGERAAAERGVLIGLTRTRVPALFARLGWAICGRHN
                     YSSAAPRAVLAELGATAAGVIDARPAAPVGLPLPPVEPIVVRPLRRIELPAIVRLYEQ
                     QSAGRRGWPIRSEAYWDWLLARGACDRIYVAATGTEPAELPKLIDSIVGYAFVRQCRL
                     VEVIAAPGRDEVARHLTARVCADASEQDDWLVRCDAPPEHPLHVLFRQAGGKVTSSQE
                     LGGEVFMARLLDPLALLRQLQPTFTARARAADVHRPSILGIELRSGCGKKDQGVIERF
                     RIQLGRRRTTIETGGPSRHTVTLRYTDLAPLLLGDQSAADLAAANRLRASTKKSNHLA
                     AALFPGNSWWRPPLDDLLA"
     gene            complement(7316..7663)
                     /locus_tag="VFB80_14245"
     CDS             complement(7316..7663)
                     /locus_tag="VFB80_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34985.1"
                     /translation="MKFISTVFGIALLACLAGCGAAANQAKLRNDLKGLGLAYHSYHD
                     EHQKGPANWDELIAFAGKANLFPDGIKRVRDAGYVVTWDAKFTEVTQGLSNTVLAKPA
                     GEGPTLMMDGSVQ"
     gene            complement(7695..7767)
                     /locus_tag="VFB80_14250"
     tRNA            complement(7695..7767)
                     /locus_tag="VFB80_14250"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7732..7734),aa:Lys,seq:ttt)
     gene            8005..8160
                     /locus_tag="VFB80_14255"
     CDS             8005..8160
                     /locus_tag="VFB80_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34986.1"
                     /translation="MPLALGPQTIIVPIEAYLGMGFVALAIIVVPTLLIWYTIREQRR
                     IAARGRE"
BASE COUNT         1356 a         2829 c         2758 g         1536 t
ORIGIN      
        1 ttgaccaggg cgaagtagtc gtcgctgaac cgcttgacct ccttgatctt ggccttgtcc
       61 ttctcgggat ccacgtcctt gcagttgttg gcaacccagg tgttgccgcg cttgtagagc
      121 gtctccttgc cgacgttctg cacgctggag gcataaacct cgcggtcgct gtcgatgtcg
      181 cggaagtagt tcgacacgcg gccgccgtca gggctgccat agccgccacc aaggccgccg
      241 ccagcgatag ctgctccggc ttgaccctga aatccgctct tcgcgcgggc cgccaatgac
      301 gctgccggcg taggagcgcc cggcgcgccc agccccgcac cgcgggcctc gcccgccggc
      361 tgcgagaacg acggcgccgc ggcctgcgcg gcccgttcgg cgttttgcag cacgttctta
      421 tccgaccgct gcgagaagcc ggcgatgccg tcggccgtgc tcagccggtc cgtctcgcgg
      481 cgggcgtaat ccaaatgcag cctcgtatcg gccagctgcc gcaccggggc gttttcgtcg
      541 gccaggaacg acgtatacgg agtgaggatg ccatgtttcg tcgagagtcc gaccagctcc
      601 ttcaagagct cgtcgttctt ccccttgagg tccatctcgt cgatgatctc gccgatccgc
      661 cgcatggccc acagcttttc gacgaaggca aagctctggt cgcccgactt ctcggtgaag
      721 ctccctggga agtcgtgctt ccgctcatcc ttgccgactt tgcccgagat cgtgaccttg
      781 cccatgccgt acttcttgta gcggccgacc attaccagct gctcgccggc aaacaggtca
      841 tagacttcct tgggatagac gcggctcact ccgcccgact cggagcccgc cccttcgatg
      901 tcgatcttca cggcgacatt ggtcagcacc ggggcgctca tcttggcgta cagccggctg
      961 acgtgggcct cgatgttctc gtcggggcgg acgtagccgc tctggccaaa attgtcgcgc
     1021 gacaggcggt cgagcagccg gctgttgacg tcgtaaccga cgcccatgtt gatcatccgg
     1081 gtgcggacct tgttgttctg cttcgcgttg gcggcgatct tggcctcgtt agtctcgccg
     1141 gcggtcggca ggccgtcggt gaggaagaaa acaaagttcg gccgcgagtc gtccttgatc
     1201 atcgagaggg ccgtgctcag cgccccgtcg atgttcgtgc tgccgccggc atagatcccg
     1261 tccacgaagc cgagggccgc tttgcgcgtc tcatcgtcga acttttgcag ctccggcttg
     1321 aagctttcga cgttgctgtc gtaggccacg atgttgaacg tgtcgccctg ccgcaggttg
     1381 ttgagcacga acttcagcgc ttccttggcc tgctcgatct tcttgccgct catgctgccc
     1441 gagcgatcga cgacgaagat caccgtcttg gccggccgct cgtcgctggt ggctttgatc
     1501 tccgggctgg ccagcagcag gaaaaagccc tcgtcgctgc tgtcgggccg gtagctgatc
     1561 aggctcgcgc cgagcgagcc gtcgttgacg tcgtagagca ggcggaaatc cgtcgtgggc
     1621 acggtgttcg tggcctcgaa cttgacgacc gcatgcttgt tgtccggccg ctggatgctc
     1681 acgttgtgcg tcgggctata gacgctcttg atttcctgcg acgattcgat cgcggcatgg
     1741 atcgagagct tctcgaccgg cgacgacgtg tacttcgccg tcgagagcgg aatcatcagg
     1801 tcggtgagct tctggtcctt ccgcaggagt tggctgaact tgagcgagac tttgcgctcg
     1861 gcccccgccg gcaccgggaa gacgctggtc tggaacatgc cgtagccgac ccattccagc
     1921 agggccgggt cctggttgcg gcggacgtag ccttcgtaaa tccggcgggc ctcgtccttg
     1981 gccagcagct tggcgtcgta ctccttgcca tcgaccatga aggtcatccg gtcgatcgcc
     2041 ccgtcgtagg gaatcggaaa gacgaatgag acctccatcg cggcgctgcc ggtgttgaca
     2101 aacgactgcg tcacctgcac ctgggcgacc tggtcaacga tcttggcgtg gtagtccagc
     2161 tccttgatct tgtacgacat cggcggcggc gtcggccgcg gacggggaat cggccgcggc
     2221 agcggcactg ggtgcggcgg attgatcacc accagcacgc cctgtccaaa agcaatgctg
     2281 cccagaagca gaaatgccaa gcccgccaga gagatgcgtt tcatggccaa attcctgtaa
     2341 gacgccagag aatgccgcct gtaaagatag gcaagccgcc gcccgcacgt gtggtcgcaa
     2401 actattcgta tgacgactct tgcccgccca tagttttcgc acctccccat cagccggcaa
     2461 gccggcgtta cggtatcgtt acgcgactgg cttcggcaac gccccggtta ggtggactgc
     2521 actcgctctc tgacgcttaa ttttcgccgc aacccctggc agctcaagat gttgtggcga
     2581 acgtgctgcg aaccccttaa gtactactta ttagtgtttg agatttgcat tggccggctg
     2641 gccccgcgcc aaaatggcag gccgcagcgc agctgaaaca ctactgcaaa tagagtacca
     2701 aagttcacta atcggttacg ggccagttac ccgcgaggca ttccctccca gccgccggag
     2761 cgcccataat ttcgcagagc ccttgcccgg cgactctccc ctttcctgcc ctggacgatt
     2821 cccatgcgta acacttcgat ctgcaccgcg gctgtcgttt catgcttggc gctggcgtcg
     2881 gcttctggcc aggagtggcc ccagatgctg gggccgaatc gcaatggcat ctcggccgag
     2941 acggggctgc tcgaatcctg gcccgagggc gggccgaagg tcctgtggcg cgtgccgggc
     3001 ggcgtcggca tgtcggggct ggcgatcagc cgccttaagc tgctgacgct ggtgcaaaag
     3061 gacggccagc agttcctgac ggccaagaat gccgagacgg gagcgccgct gtgggagacg
     3121 ccgctggcca gcgagtacga gaaccagatg ggcaacggcc cgcgggcgac tccgacgatc
     3181 gccggcgagc gcgtcttcgc ctacacgggc gagggcgtgc tcgtggccct gaacttcgcg
     3241 gacggcaagc tgctctggaa gcacaacgtc cccgtggagc tgaagggcca gcccgcggac
     3301 tatgggatgg cttgctcgcc gctggttgtc ggccagcacg tgattgtcca ggccggtgct
     3361 ccgcaggcgg cagtcgcggc gtacgacgcc gcgagcggca agctcgcctg gactgcgggt
     3421 gaagatggcg cgggctactc gtcccccgcg ctgctcgacg tcggcggcca gcagcaggtc
     3481 gtcgccttca cgggtggctc ggcaatcggc ctcgctcccg agaagggcac gctgctgtgg
     3541 cgctatccat acgagaccga ttaccagtgc aacatcgcca cgccgctggc ccaccgcggg
     3601 caggtcttcc tctcgtcggg cgaaaatcac ggctgcgtgc tgctgggcct gcgggccgac
     3661 ggcgagaagt tcgtgccgcg cgtgatttgg gagtcgcaag gctccgaaag cgtgctccgc
     3721 aacgagtggc agacgtcgat cttgttggac gggcatctgt acggcttcga caacatcggc
     3781 agcgccgggc cggtgacgca cctgacctgc gttgacgtgg ccacgggcaa gcgcgcctgg
     3841 cagcagacgc gttttggcaa ggggaacctg atcgccgccg acggcaagct gttcatttcc
     3901 accatgaagg gcgaactggt cgtcgtccgc gccacgccca aggcctacga ggaaattggc
     3961 cggctggcga tcctcggctc gacgcgccag gcccccgccc tggccggcgg ccggctctac
     4021 ctgcgcgacg acaaggaaat cgtctgtctc gacgtacgga agaagtgacg atccggccgc
     4081 aggacgccgc cggccatcgc gggtagaata gcgcctcccg ccatgccgcc gctcgtccaa
     4141 cgccgccttc gcgcccccgc cgacgacggc agcgcgctgt ttgacccgcc gctgtcgcaa
     4201 gcccccgcgc tgatcgagcg caatcgggcg gtcgcttcga aactcgagaa gcacgggatc
     4261 agcgctcgcc tgcgcgtgac ggcgcgaatc atcctgctcg gctgcgcgga gatccgcgac
     4321 ggagtgcaac tcgagcgcga ggaactgcag cggccgatca tcctcgccgg acaccagccg
     4381 gagctgtacc atccgggcgt ctggttgaag aacttcgccc tgtcgtcgct cgcgacgtca
     4441 gtcggcgcga ccagcctggg cctgatcatc gacaacgaca ccgtccgcag cacttcgatt
     4501 cgcgtgccgt cgggcggctc gccgccggtg gtcgaggtcc ccttcgatgc gccagccgat
     4561 gcgattccct gggaagcgcg gtgggtcagc gacccggcga agtttgcaaa gttccgcctg
     4621 gaggtgcgcg gagcatttgg atcgttcctc aagtcgccag cgtacaaaca cggattgctc
     4681 ctggatcgcg tttggccgca cgcgctggca gcggccgacg agcagcgcga aatggccaag
     4741 aggtcgctgg agaaactccg gcgcggcgga ccgcatccgg agctgctcgc gcaagccgtc
     4801 aatctgggcg aatgcctggc gaccgcgcgg catcgcgtcg agcgcgagct cggcctggtg
     4861 caccgcgagg ccgccctggg cgcggtgacc ggccagcccc ctttcctgga atttgcccac
     4921 cggctgctag cgcggcatcg cgagtttcac gcggtccaca acgcggcctt ggccgaatac
     4981 cgggcagtaa atcacatccg cagccagacg catcctgtcc ccgacctggc ccaggacggc
     5041 gactggcacg agatgccgct ctggatttgg acaatggacg atccgcgccg acgccgcgtc
     5101 ttcgttaggg aggctgccag ctcgtgggag ctgaccgatc ggcaggggct ggttattgac
     5161 ggcccagccg ccgggcagga ctgcgacgag gtgtactccc gcttgcttcg ggaagggatc
     5221 gcgcttcgcc cgcgggccct gatcacgacg atgttcgccc ggctggtttt gagcgacctg
     5281 ttcatccacg gcatcggtgg ggcgaagtac gacgagctga ccgatttgat cgttcgccgg
     5341 tttttcggcg tcgagccgcc ggcgttcatc acggccaccg caacctttcg gctgcccatt
     5401 gagcgccttc agatttcgct ggacgacgtg cgccgctcgg ccgcgcgaat tcgcgacctc
     5461 cgctggcatc cggagacctt cgcccgcaat ccgctggtcg cgggtgagcc gtcactggct
     5521 gagaaactcg cggcgctggc ggcggaaaag cgtgagtatc tcgcgcagca cgacctgcgg
     5581 cgctgcccgc cggaggtctt cgcgcgggcg gatgcgctca atcgcgcgat gcacgcgctg
     5641 ctcgggccgg tcgagcgcgc gctgcgcgcc gagcacgctg aattggtcga gcagttcaag
     5701 cgcgcgcagg tcctgggctc gcgcgagttt tcattcgtac tgttcccttc agaaattctt
     5761 tccgcgcgcc tactagattt gtgcaaggtt tcgtcgtaac ttgttagcaa cttcgcggct
     5821 cacgacgagc ctgctggaag gattcaacaa ggcaaaaggg actgcctgca tggccacgcg
     5881 caccgtttcg gtcgcacgac gctcgccatc catcactctt accaagcctc gcccgacagt
     5941 tgccgccccc ggtctgcggc tcacgccggc ccgcaccgga gatcacgggc tgatccaccg
     6001 gctcctggtt tccgtctttc atggcccgtc ggccggcgaa ttccacgctc agctcgacga
     6061 gccgggctac gaaccagccg accggctcgt cgtgaaagac ggcgagcaga tcgcggccca
     6121 cctgcggctg gcgcggcaga cgatccgcgt cggcgaagcg gcgctgcccg cggcgcggtt
     6181 catggacctg gcgaccgcgc cggagtatcg cagccgcggt ctggcgacga cgatgctggc
     6241 ggccggcgag cgggcggcgg ccgagcgggg tgtgctgatc ggcctgacgc ggacccgcgt
     6301 gccggcactt ttcgcccgcc tggggtgggc gatctgcggg cggcacaact attcgtccgc
     6361 cgcgccgcgg gccgtgctgg ccgagctggg cgcgacggcc gccggcgtga tcgacgcgcg
     6421 gccggccgcg ccggtgggct tgccgctgcc gccggtcgag ccgatcgtcg tccgcccgct
     6481 gcggcggatc gagctgcccg ccatcgtgcg tctctacgag cagcagagcg cggggcgccg
     6541 cggctggccg atccgcagcg aggcctattg ggactggctg ctggcccgcg gagcgtgcga
     6601 ccggatctac gtcgccgcca ccggcaccga gcccgccgag ctgcccaagc tgatcgactc
     6661 gatcgtcggc tatgcgttcg tccgtcagtg ccggctggtc gaagtgatcg cagcgcccgg
     6721 ccgagacgaa gtcgcgcgtc acctgacggc gcgggtctgc gccgacgcca gcgagcagga
     6781 cgattggctc gttcgctgcg atgctccgcc cgagcatccg ctgcacgtgc tcttccgcca
     6841 ggccggcggc aaggtcacct ccagccagga gctgggcggc gaggtcttca tggcccgcct
     6901 gctcgacccg ctggccttgc tccgccaatt gcagccgacg ttcaccgcgc gggcccgcgc
     6961 ggccgacgtc caccggccca gcattttggg aatcgagctg cgcagcggct gcggcaagaa
     7021 ggaccagggc gtgatcgaac gcttccgcat ccaactcggc cgccgccgga caacgatcga
     7081 aacgggcggc cccagccggc ataccgtgac gctccgctac accgacctgg cgccgctgct
     7141 gctcggcgac cagtcggccg ccgacctcgc cgccgccaat cgcctgcgag cctcgaccaa
     7201 aaagtccaat cacctcgccg ccgccctctt ccccggcaac tcgtggtggc ggccgccgct
     7261 cgatgatctg ctcgcctaat cgcgacgacc tgcccgcctg gtttgttcgc gagcgctatt
     7321 gcacgctgcc gtccatcatc agcgtgggac cttcgccggc cggcttggcc aacaccgtat
     7381 tcgacagtcc ctgcgtcacc tccgtgaact ttgcatccca tgtcaccaca tatccggcgt
     7441 cgcggacccg cttgatgccg tcggggaaca ggttcgcctt gccggcgaac gcgatcagct
     7501 cgtcccagtt ggccggtcct ttctggtgct cgtcgtgata cgagtggtag gccaggccca
     7561 gtcccttgag atcgttgcgg agcttcgcct gattcgctgc cgcgccgcac cctgccaggc
     7621 aagccagcag tgcaatgcca aaaacggtcg atatgaactt catcgattcc tccgcgggac
     7681 gcgatccaaa aatgtgaggc tgtcggggct cgaacccgag acctacggat taaaagtccg
     7741 ttgctctacc gactgagcta cagcctctaa gcggcggcct cgtaatgagt ggcgtcgtga
     7801 gcgggcggga gatctaccgc acgatcgggc cgcttcgtca gtctagccga atcggggcca
     7861 gcgacaagcc aagcgaactg aaccaccggc ggcccgctgg catcgactaa aaagcgtggt
     7921 tcagcgctcg cgacaaccaa gcacaacctt cgcgccgtcc gcgtcggctg gcggggcttt
     7981 ttcgtgcggg ggtagaatag gcaggtgccg cttgcccttg gccctcagac gatcatcgtg
     8041 cccattgagg cctacctggg catggggttc gtggccctcg cgatcatcgt ggtgccaacg
     8101 ctcttgatct ggtacacgat ccgggagcag cggcggattg cggcgagagg ccgcgaatag
     8161 tagaggtagc gagaaccgaa agtctcgatc gctaacccat gcgctgccgc ctccgcacac
     8221 tgctgatcgt cttggcgctg gggccgccgg cgctggcggg cctttggtgg ggataccgcg
     8281 attggcaggc acgtcgtgcc gcagcattgg agcgccaagc agtgtttcag ttctacatcg
     8341 gcataacacg gtgaatggag ccgtcgatga tgcgattcac gatccgcgag ctggtgctgg
     8401 tgacggtcat cgtggcaatg ggcgccgcgt ggtggattga caggcgggcg cagttcagcg
     8461 acgcgaatag tcgcgccga
//
LOCUS       DASRJB010000548         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4101737, whole genome shotgun sequence.
ACCESSION   DASRJB010000548 DASRJB010000000
VERSION     DASRJB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4101737"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFB80_14260"
     CDS             complement(<1..277)
                     /locus_tag="VFB80_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34987.1"
                     /translation="MSLPPRPDFEPDDEDPRPTRRAGPLLAWLRLLRLPNVFTALADV
                     AMGYLFVHRSVPSPAQLICLAAASGLLYSAGMVLNDVFDLEVDSRERP"
     gene            complement(286..1269)
                     /locus_tag="VFB80_14265"
     CDS             complement(286..1269)
                     /locus_tag="VFB80_14265"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZN34988.1"
                     /translation="MSADVVVRRKKRKSPAHRRPIKQRRDHSPDFPPSTASHRLIMGL
                     AVALPFLGCIAAAGLLWQWGFMGWLYVGMLVGGWLLTGTGITIGFHRLLTHRSFDTYR
                     WVKLVWMALGALSVEGSPLVWCAVHRRHHEHSDQPGDPHSPHLHGSGLIGTLRGLWHA
                     QTGWLFTGYWTSPELARYVPDLLADPALVAVDRMYYVWVLLSLFLPTLIGGLATWSWQ
                     GALLGLLWGGLVRIFITHHITWSINSICHIFGSRDFAAGDDSRNNWLCGLLGLGEGWH
                     NNHHAFPTSARHGLAWWQLDASWLIIRGMELVGLAWNVRLPNERAIAVKRL"
     gene            complement(1391..1831)
                     /locus_tag="VFB80_14270"
     CDS             complement(1391..1831)
                     /locus_tag="VFB80_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZN34989.1"
                     /translation="MAKRIYVGNLPWSYGSTELEQLFAQHGQVAAAEVIMDRETGRSR
                     GFGFVQMANDADMEGAINALNGFNCGGRPLVVNEARERTPRAGGGGGGYGGGGGGRGG
                     GGGGYGGGGGRGGGGYGGGGGGYGGGGGGYGDREGGGGGGGRRY"
     gene            complement(2123..2779)
                     /locus_tag="VFB80_14275"
     CDS             complement(2123..2779)
                     /locus_tag="VFB80_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34990.1"
                     /translation="MVSLGKWIVAGSIGGAAGAAVWAMIAYFAHAEVGWIAWGIGAVV
                     GICIRFVSGESQEGLGPGIAAALIAILSVLGGKYAAVSMLVASLDMGVETLELTDEQM
                     IATLADEIVGELQAKGKKVVFPAGKTLEDASGQADYPPAIWKSAAQRWTALGPAKQKE
                     RREEQQKQYAAVMGAVRGALHERAFAESFSLYDALWFFLAAATAFRLGAGNVGSEDDD
                     "
     gene            complement(2883..>3505)
                     /locus_tag="VFB80_14280"
     CDS             complement(2883..>3505)
                     /locus_tag="VFB80_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN34991.1"
                     /translation="VPVAMHLAETREELELLESHSGPLVEMLGSLGAWQPTALSRGLR
                     PLDYLRTLATAHRALVIHGNYLAADEIEFAAAHRDRMSVVYCPRTHAFFRHEPYPLAK
                     MRDAGVRVAVGTDSRASNPDLNLWSELRHIAEHHRGVSPDEILRMGTLAGAEALGLDG
                     DLGSITPGKSARLVMASLPAGGQAALAAILESASTIVPVHLSHDEN"
BASE COUNT          679 a         1183 c         1106 g          537 t
ORIGIN      
        1 acggccgctc gcggctgtcc acctcgagat cgaacacgtc gttgagcacc atgcccgccg
       61 aatagagcag gcccgaagcg gcggcgaggc agatgagttg agcggggctg ggaaccgagc
      121 ggtggacgaa gagatagccc atcgccacgt cggccagagc ggtgaatacg ttggggagcc
      181 gcaacaggcg gagccaggcg agcagcggac ctgcgcggcg cgtggggcgg gggtcttcgt
      241 cgtcgggctc gaaatcggga cgaggcggca gggacaaggc gtcaactaca gtcgtttaac
      301 agcaatcgcc cgttcgttcg gcagacggac gttccaggcc agaccgacga gctccatgcc
      361 ccggatgata agccagctgg cgtcgagctg ccaccacgcc agcccgtgtc gagcggaggt
      421 ggggaacgcg tggtggttgt tgtgccagcc ttcgcccaga cccagcaggc cgcagagcca
      481 gttgttgcgc gagtcgtcgc cggccgcgaa atcgcggctg ccgaagatgt ggcagatcga
      541 gttgatcgac caggtgatgt ggtgggtgat gaagatccgc acgaggccac cccaaagcag
      601 tcccagcaaa gctccttgcc acgaccaggt cgccagtccg ccgatcaacg ttggcaggaa
      661 aaggctgagc agcacccaca cgtaatacat ccgatcgact gcaacgagcg ctgggtcggc
      721 caataaatcg ggcacatagc gggccagctc gggagacgtc caatagcccg tgaagagcca
      781 gccggtttgc gcgtgccaca ggccgcggag cgtgccgatc agcccgctgc cgtgcaagtg
      841 cggcgaatgc ggatcgcccg gctgatcgct gtgttcgtgg tgccgccggt gcactgcgca
      901 ccagaccagc ggcgagccct cgaccgacag ggcaccgagg gccatccaga ccaacttcac
      961 ccagcgatac gtgtcgaaac tgcggtgcgt gagcagccgg tgaaatccga tcgtgatgcc
     1021 cgtgccggtc agcagccagc cgccgacgag catgccgacg tagagccagc ccatgaagcc
     1081 ccattgccag agcagaccgg ccgctgcgat gcagccgagg aaaggcagcg cgaccgccag
     1141 gcccatgatg agcctgtgac tggccgtcga gggagggaaa tcggggctgt ggtcgcgtcg
     1201 ctgctttatg ggacgccggt gcgccggcga cttgcgtttc ttgcggcgga cgacgacgtc
     1261 ggctgacact cgggatcctt cagcaatggc gcaagcgcca gcggactgcc gcgttttttg
     1321 cctgcggcag tcacagttgg caacatgctt ggacgaagaa ccgtccaagc ctcgctttga
     1381 acggagggaa ctagtagcgc cggccgccgc caccgccgcc gccttcgcgg tcgccgtagc
     1441 cgccgccacc gccaccgtaa cctccaccac caccgccgta gccgccgcca ccgcggccgc
     1501 caccaccgcc atagccgccg ccaccgccgc cgcggccgcc accaccgccg ccatatccgc
     1561 cgccgccgcc gccagcacgc ggcgtgcgtt cgcgggcttc gttcaccacg agcggccggc
     1621 cgccgcagtt gaacccgttg agagcgttga tcgctccctc catatccgcg tcattcgcca
     1681 tctgcacgaa cccaaacccg cgcgaccgtc cggtctcgcg atccatgatc acctcggcag
     1741 cggccacctg gccgtgctgc gcaaacaact gttccagctc cgtagagccg tacgaccagg
     1801 gcagattgcc cacatagatc cgcttcgcca tccgacaggt ccttacaaaa accacggtgg
     1861 aaaatcaaca gttgcccggc gctaacctgg aagcggcggg gagcgcggcg cgtcgtttcc
     1921 gaaggaccct gccagcagac gagtggaagc aacccgatcg gcagacaaac aaggaccgga
     1981 cacaaaacag cctgacaaga ctccgccaac ctccacgcga ggtcggcaac tgttacctcc
     2041 gcgaactata cccgctagca gggtgcaatg aaagagcatt ccgtcaagat tacgcggaag
     2101 cggacgctag cggccgatgc gactagtcgt cgtcctcaga acccacgttt ccggcaccca
     2161 atcggaaggc agttgcggcc gccaggaaaa accagagggc gtcgtaaaga ctgaagcttt
     2221 cggcaaaagc ccgctcgtgc aacgcgccgc ggaccgctcc catgacggca gcatattgct
     2281 tctgctgctc ttcgcgtcgc tccttctgtt tggccgggcc cagggcggtc cagcgctggg
     2341 ctgcagactt ccagatggca ggcggataat cggcctggcc gctagcgtct tccaaggttt
     2401 ttccggcagg aaagacgact ttctttccct tggcttgcag ttcgcccacg atctcatcag
     2461 ccagcgtggc aatcatctgc tcgtcggtca gttccagcgt ttccacgccc atgtcgagcg
     2521 aggctacgag catgctcacg gcggcgtact tgccgccgag caccgacagg atcgctatca
     2581 gcgccgcggc gatacccggc cccagccctt cttgcgactc gcccgacacg aagcggatgc
     2641 agatgccgac gaccgcaccg atgccccagg cgatccagcc gacttcggcg tgcgcaaaat
     2701 aggcgatcat ggcccacaca gcggctccgg ctgcaccgcc gatcgaaccg gccacgatcc
     2761 acttgcccaa gctgaccatc ggcgtgttgc ctttccgcga atcgaagttc cccctcgagt
     2821 cgtccgacga ctgcgagcat cgtagttgcc cgcctacgta gtgggaagaa gcggcggcga
     2881 attcagttct cgtcgtgcga taaatgaact ggcacgattg tgctggccga ttccaggatc
     2941 gccgcgagcg ccgcttggcc gccggccggc aaggaagcca tgacaagccg ggccgacttt
     3001 cccggtgtaa tgctgcccag atcgccgtcc agcccaagcg cttcggcgcc ggcgagcgtg
     3061 cccatccgca ggatttcgtc gggcgagacg ccgcggtgat gctcggcgat gtgccgcagc
     3121 tcggaccaca ggttcaagtc cgggttgctg gcccgcgaat cggtgccgac ggcgacgcgc
     3181 acgcccgcgt cgcgcatctt ggccagcgga tacggctcgt ggcgaaagaa cgcgtgcgtc
     3241 cgcgggcaat agacgacgct catgcggtcc cgatgcgccg cggcgaattc gatttcgtcg
     3301 gcagcgaggt aatttccgtg aatgaccagc gcgcggtgcg ccgtagccag cgtccgcaga
     3361 taatcgagcg gccgcaggcc gcgcgaaaga gcagtaggct gccaggcccc cagcgagccg
     3421 agcatttcca cgagcggtcc ggaatgcgat tccagcagct ccaattcctc gcgcgtctcg
     3481 gccaggtgca tagccaccgg aactt
//
LOCUS       DASRJB010000549         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1280062, whole genome shotgun sequence.
ACCESSION   DASRJB010000549 DASRJB010000000
VERSION     DASRJB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280062"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1178)
                     /locus_tag="VFB80_14285"
     CDS             complement(270..1178)
                     /locus_tag="VFB80_14285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN34992.1"
                     /translation="MGVLLALLAAAYVHYYLYLPMGSGPAGPPVAAAPFEQPWTERQV
                     LLVGIGDSVTAGLGSSRGRSYFERLVRNPPDEFGDMQGRSLSRVLPNLKTLNIAVSGS
                     TSLHHLEHIRQKLPPQKPDVFGLVVMTTGGNDLIHWYGRSPPREGAMYGATLAEAQPW
                     IASYEQRLDTMFGEIESRFPPGGCAIFVADIYDPSDGLGDPESTWALPAWPDCLAIHR
                     AYNAALARVVAKHAAAHVVPVHDEFLGHGIHCRQFWRARYDSADPHYWYFENIEDPND
                     RGYDALRRLFLNEIVRHRGALADPEK"
     gene            1481..2800
                     /locus_tag="VFB80_14290"
     CDS             1481..2800
                     /locus_tag="VFB80_14290"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34993.1"
                     /translation="MRHLPRLAVGTIQPEARGEPVLWGLLAALAAADDSPVLFHSSCC
                     FAPHDPAKTILGRSARHLDSWAMSRDAAAAALARATNDRDLAIVAGAYHDPGCTSNLN
                     TLCEWLDLARIAIVDVGCLRQRGWAMPPAHVDALLLDRVNTAEEAAYWQTTLEALWKT
                     PVLGWLDEARALRELCTSLPAGANPSRELGEALGARLLLNLRRDKLHALARRAAPLTV
                     DAEDFIPRGERSGQRRFRIAVAFDQAYCGYYPETLDLLEAAGAELCDFSPLKSEALPD
                     GADVVYFGCGHPERDPEALARNHCLKQSLRCYAAVGGRIYAEGSGLAYLCREAVFDGG
                     RTVPMTGLLPATARWLPQAGPPEPTEVVFGASSWLVPAHVSLRGYRHTGWQIEPRGPM
                     LTYAADPAQRLDILGRGNVIGSRILVNLAANRHLLRRFFEPYAPAVS"
     gene            complement(2852..3205)
                     /locus_tag="VFB80_14295"
     CDS             complement(2852..3205)
                     /locus_tag="VFB80_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34994.1"
                     /translation="MAVAAPNGTILQIAYQCTQFSRGILKRRKSASGRTDSSGQYTLT
                     TYFSPDDQPAGAMPGEYVVTVTKIDEPEGIVDPYKKGQMPKNQLPDKYSSQRSPLSAT
                     VTAGGANRFDFPLED"
     gene            3652..>4746
                     /locus_tag="VFB80_14300"
     CDS             3652..>4746
                     /locus_tag="VFB80_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN34995.1"
                     /translation="MCTRAHPRFERIRRGFTLVELLVVIAIIGVLVALLLPAVQSARE
                     AARRMSCQNNLKQLALACHNFEDTFKSLPAGNVYRPDAQGKNNYYETWTISILPFMEQ
                     ANLYTSHYDQNVPNAITDANSPTKMAILRKTVLKAHICPSDPTDMKKPAIPDSGPGGD
                     TGLPRNLHAPSNYRGVAGVSFGGRSGVDDSGGDANWDDATQVAWLANWNRGYRGPLHA
                     CTNFFPSLTPPVPPPNLNAVRLAEVTDGTSNTLMIGEYATKPFTDANGVLHDRRGFWA
                     YAYTSYNLSGITINQSRIFIPDFVKCSATPPTRSNNQCKRAWGSFHGGGGVNFAMTDG
                     SVRGISPNIDVNGVNAPFVALGSIGGGETVQ"
BASE COUNT          815 a         1573 c         1513 g          845 t
ORIGIN      
        1 tgtaaaccgg cgtgtagcac ggctggcagt gggtcacggg aaaacagccg ccgtgatggg
       61 cctgggcggc cgacggcagc gagagcaggg cgagcgcggc ggcgaagagc gcgccgcgaa
      121 cgagcgattg gatgcggaac atgtctggat ctcctgggcg gaggttgtgg ttggacttcg
      181 ccgggcgaca ctgcccagcg tgcatttatt gcgaagcgta gagccgccgc ggttgttaca
      241 acccgcagca ctttgctaga caatcgccgc tacttttccg gatccgccag cgccccgcga
      301 tgccgcacga tctcgttcag aaacagccgc cggagggcgt cgtatccgcg gtcgttcggg
      361 tcttcgatgt tctcaaaata ccaatagtgc ggatcggcgc tgtcgtaccg cgcccgccag
      421 aactgccggc agtgaatgcc gtggcccaga aactcgtcgt gcacgggaac gacgtgagcc
      481 gcggcgtgct tcgcgacgac gcgggccagc gccgcgttgt aggcccggtg gatcgccagg
      541 cagtcgggcc aggcgggcag ggcccacgtg ctctccggat cgcccagccc gtcggacgga
      601 tcgtagatgt cggcgacgaa gatcgcgcag ccgccggggg ggaaccgcga ctcgatctcg
      661 ccgaacatcg tgtccagccg ctgctcgtag ctggcgatcc agggctgcgc ctcagccagc
      721 gtcgcgccat acatcgcgcc ctcgcgaggc ggcgagcggc cataccagtg gatcaggtcg
      781 ttgccgccgg tcgtcatcac gaccaggccg aagacatcgg gcttttgcgg cggcagcttt
      841 tggcgaatgt gctccaggtg atggagcgag gtgctgcccg agactgcaat gttcagcgtc
      901 ttcaagttcg gcaagacgcg cgacagcgac cggccctgca tgtcgccgaa ctcatccggc
      961 ggattgcgaa cgaggcgctc gaagtacgac ctgccgcggc tggatcccag tccggccgtc
     1021 acactgtcgc cgattcccac cagcaggacc tgccgctcgg tccacggctg ctcgaacggc
     1081 gcggcggcaa caggcggccc ggcgggtccg ctgcccatcg gcaggtagag gtaataatgg
     1141 acgtacgccg ccgccaggag agcgagcaaa acccccacac ccagcagcca tgccagccgt
     1201 cgccaacggc gccagcgagc gggagtctac ttggcagccg gggcgcaaga agttgacatt
     1261 tcaacgattc tacagggaaa aacgggacat ttttgcgtgc ggttttccgc actaccggca
     1321 cgccgcgccg ttgactctcc acttcagccc tcgaacaatc tagcggtgag cctgccctcc
     1381 gctgccggcc gccggtttgt cctcccgcaa tcgctagcaa gcctcccgaa gccccctctc
     1441 tccgtcggct gctcagcatg acccagctct ccgaggtcgt atgagacatc tgccgcgctt
     1501 ggccgttggc acaattcagc cggaggcacg cggggagcca gtgctctggg gattgctggc
     1561 cgcgctcgcg gccgcggacg actcgccggt cttgtttcac agctcctgct gctttgctcc
     1621 gcacgatcct gcgaagacga tcctcggccg ctccgcgcgg cacctcgaca gttgggccat
     1681 gtcgcgcgac gccgccgcgg cggcgctggc gcgggccacg aacgaccgcg acctggcgat
     1741 cgtcgccggc gcttatcacg acccgggctg cacgagcaac ctgaacacgc tgtgcgagtg
     1801 gctcgacctg gcgcggatcg cgatcgtcga tgtcggctgc ctgcggcagc gcggctgggc
     1861 aatgcccccg gcccacgtcg atgcgctcct gctcgaccgc gtgaataccg ccgaggaggc
     1921 ggcctattgg caaaccacgc tcgaagcgct gtggaagacg cccgtgctcg gctggctcga
     1981 cgaagcccgc gcgctgcggg agctgtgcac gtcgctgcct gccggggcga atccgtcgcg
     2041 cgagctgggc gaagcgctgg gcgcccgcct gctcctgaat ttgcggcggg acaagctgca
     2101 cgcgctggcc cgccgcgccg cgccgctgac ggtggatgcc gaggatttca ttccccgcgg
     2161 cgagcgctct gggcagcgcc gcttccgcat cgccgtggcc ttcgaccagg cgtattgcgg
     2221 atactacccc gagacgctcg acctgctcga agccgccggc gcggaactgt gcgacttttc
     2281 gccgctcaag agcgaagccc tgcccgacgg ggccgacgtt gtctatttcg gctgcggaca
     2341 tcccgagcgc gatcccgaag cgctggcccg caatcactgc ctcaagcaat cgctccgctg
     2401 ttatgccgcg gtcggcggac ggatttatgc cgaagggagc ggcctggcct atctctgccg
     2461 cgaggccgtc ttcgacggcg gccgcacggt gcccatgacc ggcctgttgc ccgcgacggc
     2521 acgctggctg ccgcaggccg gcccgccgga gccgacggag gtcgtcttcg gcgcgagttc
     2581 ctggctcgtc ccggctcacg tctcgctccg cggctatcgc cacaccggct ggcagatcga
     2641 gccccgcggc ccgatgctca cctacgccgc cgaccccgcc cagcggctcg acatcctcgg
     2701 ccgcggcaac gtcatcggca gccgcatcct ggtcaacctc gccgccaacc gccacctgct
     2761 gcggcgattc ttcgagccgt acgcccccgc ggtgtcgtag ggctgtcctg tacgttgggc
     2821 tgtgcccacc tgtcgagcgc gagtctgcgc ttcaatcctc aagcggaaag tcgaagcgat
     2881 tggcgccgcc ggcggtgacc gtggccgaga gcgggctgcg ctgcgagctg tacttgtcgg
     2941 gcagctggtt cttcggcatt tggccctttt tgtagggatc gacgatcccc tccggctcgt
     3001 cgatcttcgt caccgtcacg acgtactcgc ccggcatcgc cccggccggc tgatcgtcgg
     3061 ggctgaagta cgtcgtcagc gtgtactggc cggaggagtc ggtgcgcccg ctggcactct
     3121 tccggcgctt cagaattccg cggctgaatt gtgtacattg gtacgctatt tgcagtatcg
     3181 ttccatttgg cgcggcgact gccattttgg ccccgccaca ccggaaaatt cggtgcgcgg
     3241 tcacctgctc caaagcgccc tctgacgttt ctcaaactgt tataagtagt atttaatggc
     3301 ctgcggctgc ctgtgccgca agccgatggc gcatcaggag ttgcgaccga aaacgtggaa
     3361 aaaggcgcga tcgcactcgc ccaaccaggg ggcggatatt tgcgccgccc aatggcgtgg
     3421 ccaatttatc gggcctcaag attggcggcg gtggagccga gttcgagcac agctcggcat
     3481 acggcattcc gcacagcgga cgcgcgggaa aagactttca catcgtggag gaattggtta
     3541 ggctaccggt tgagattcag cgttgccgtt cggcggcgct gggaaagagc attggggact
     3601 tgggtttcgt tgcggttggg gttcgcgagt acttttcaaa ggggccttcg tatgtgcacg
     3661 cgcgcacatc ctcgattcga gcgcatccgc cgcggcttca cgctggtcga gctgctcgtg
     3721 gtcatcgcca tcatcggcgt gctggtcgcc ctgctgctgc cggcggtcca atcggctcgc
     3781 gaggcagcgc ggcggatgag ctgccagaac aatctcaagc agctcgcgct ggcctgccac
     3841 aatttcgagg acacctttaa gtcgcttccg gcgggcaacg tttatcgacc ggacgcccaa
     3901 ggtaagaata actactacga gacttggacg atcagtatct tgccgttcat ggaacaggca
     3961 aacctttata ccagccacta cgaccaaaac gtccccaacg ccatcaccga tgcgaatagc
     4021 ccgacgaaaa tggcgatcct gcgaaagacg gtgctcaagg cccacatctg tccctcagat
     4081 ccgaccgaca tgaaaaagcc ggcgattccc gattctgggc ctggtggtga tacagggttg
     4141 ccacgaaacc tccacgctcc ctcgaactat cgcggcgtcg cgggtgtatc gttcggcggc
     4201 cggtcaggcg tcgatgacag cggcggcgat gccaactggg acgatgcgac gcaagtggca
     4261 tggctggcga actggaatcg gggctatcgc ggaccactcc acgcatgcac gaacttcttt
     4321 cccagcctca ctcccccagt tccgccacca aatctcaacg cagtcaggct ggccgaggtg
     4381 accgatggaa cttcgaatac gctaatgatt ggcgaatacg ccaccaagcc gttcaccgat
     4441 gcgaatggcg tgctacacga tcggcgcggg ttctgggcct atgcctacac gtcgtacaac
     4501 ctttcgggca tcaccatcaa ccagagccgg atatttattc ccgacttcgt aaagtgctcg
     4561 gcaacgcctc cgacgcgctc caacaatcaa tgcaagcgag cctggggcag cttccatggc
     4621 ggcggcgggg tcaacttcgc catgaccgac ggctcggttc gcggcatctc gcccaatatc
     4681 gacgtaaatg gcgtcaatgc accgtttgtg gcgctgggtt ccatcggcgg cggcgagaca
     4741 gtgcag
//
LOCUS       DASRJB010000550         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2409161, whole genome shotgun sequence.
ACCESSION   DASRJB010000550 DASRJB010000000
VERSION     DASRJB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409161"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFB80_14305"
     CDS             <1..197
                     /locus_tag="VFB80_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN34996.1"
                     /translation="VLEIGKFFHSKKPPIPQAETLEIYAFMEAADESKRRGGEPVEIE
                     EVIAKAQPLAAKRLAELGVK"
     gene            234..2309
                     /locus_tag="VFB80_14310"
     CDS             234..2309
                     /locus_tag="VFB80_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HZN34997.1"
                     /translation="MRSTRTMTLLWLALWSLIPLAARGDEGMWLYNQFPKQLLKQKYG
                     FEPSDAWLDHLRLASVRFNSGGSGSFVSSRGLVMTNHHVGADALQKMSTADRDLLKTG
                     FHARALAEEQKCVDLELNVLVKIEDVTAQVNAAVKPGQPPAEAQLARRAVINTIEKEQ
                     FDKTGLRSDVVTLYQGGQYQLYQFKKYTDVRLVFAPEHSIAFFGGDPDNFEYPRYDLD
                     ICFFRIYENNQPAKIEHYLKWSQAGAADGELIFVSGHPGRTNRMNTVAHLEYLRDIMV
                     PQQLDVLRRREVLLSNYSSRSLENERRAGDELFGIQNSRKARLGGLGGLQDPEVFARK
                     QAEESELRSKVESNPMLKEKYAGAWDEIAATLQKVKSFRTEFNMLQGSAFNSDLFFIA
                     RTLVRMADEDAKPNAQRLPEFGQAGRASLEQQLYSEAPIYTDLEIVKLADSLGMLMEQ
                     LGGNHELVKQVLEGKSPRVRAAELIGGSKLADVAERKKLAGGGQAAIAASKDPLIKLA
                     KAVDPRSRELRTMFEQQIEEPQRQAYAKIAAARFAVLGSTTYPDATFTLRLAFGAVKG
                     YEDEGRKIEPWTTMGGTFGHAEAHGSKDPFELPPSWLKNRDKISANTPFNFVSTADII
                     GGNSGSPVVNKAGELVGIIFDGNIQSLVLDYAFSDVQARAVSVHSASIQEALRKIYSA
                     TALADELGK"
     gene            2319..>2992
                     /locus_tag="VFB80_14315"
     CDS             2319..>2992
                     /locus_tag="VFB80_14315"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZN34998.1"
                     /translation="MRIAVGGILHETSTFLPQTTGVEDFAAGFGLFRGAAVRERFAGA
                     NMCIGGFFDAAESCGFDAVPLLWGFAYPSGLIRNADYGRLKAEFLERLAAADAERRLD
                     GVLLDLHGAMVIEGLEDGDGDVVAAVREAIGTERPIAVTYDLHGNHTPRRMQLANASI
                     GYDTYPHVDMAERGREAGQLMARTVRGEARPVTALKQIPMFWSAQRQVTAHEPMDEVL
                     RRIHEL"
BASE COUNT          574 a          986 c          987 g          445 t
ORIGIN      
        1 tggtcctgga gatcggcaag ttcttccact cgaagaagcc gccgattccg caggccgaga
       61 cgctggagat ctacgccttc atggaagccg ccgacgagag caagcgccgc gggggcgagc
      121 ccgtcgagat tgaggaagtc atcgccaagg cccagccgct ggcggccaag cgcctggcgg
      181 agttgggcgt gaagtaacaa ccaattcaga aaacgtggcc acggagcaca acgatgagat
      241 ccactcgcac aatgaccctt ttgtggctcg cactttggtc gctgattccg ctcgccgcgc
      301 gcggcgatga gggaatgtgg ctctacaacc agttccccaa gcagctgctc aaacagaagt
      361 acggctttga gcccagcgac gcctggctgg accacttgcg gctggcgagc gtgcgattca
      421 acagcggcgg ttcggggtcg ttcgtgtcgt cgcgcggcct ggtgatgacc aaccaccacg
      481 tgggggccga cgcgctgcag aagatgagca ccgccgaccg cgacctgctc aagaccggct
      541 tccacgctcg ggcgctcgcc gaggagcaga agtgcgtcga tctggagctg aacgtcctcg
      601 tgaaaatcga ggacgtcacg gcgcaggtca atgccgccgt caaacccggc cagccgccgg
      661 ccgaggccca gctggcccgc cgcgccgtca tcaacacgat cgaaaaggag cagttcgaca
      721 agaccggcct gcggagcgac gtcgtcacgc tctatcaggg cggccagtat caactgtacc
      781 agttcaagaa gtacaccgac gtccggctgg tcttcgcccc cgagcactcg atcgccttct
      841 tcggcggcga ccccgacaac ttcgaatatc cgcgctacga cctcgacatc tgcttcttcc
      901 gcatttacga gaacaaccag ccggcgaaga tcgagcacta cctgaaatgg agccaggccg
      961 gcgcggccga cggcgagctg atcttcgttt ccgggcatcc cggccgcacc aaccgcatga
     1021 acacggtggc ccacctggaa tacctccgcg acatcatggt cccgcagcag ctcgacgtgc
     1081 tccgccgccg cgaggtcctg ctctcgaact acagcagccg cagcctggag aacgagcgcc
     1141 gcgcgggaga cgagctgttc ggcattcaga acagccgcaa ggcccgcctg ggcggcttgg
     1201 gcggcttgca ggacccggag gtttttgccc gcaagcaggc cgaggagtcg gagctgcggt
     1261 cgaaggtcga gtccaacccg atgctgaagg agaagtacgc cggcgcctgg gacgagatcg
     1321 ccgcgacgct gcaaaaggtc aagagcttcc gcaccgagtt caacatgctg caaggcagcg
     1381 ccttcaactc cgacctgttc ttcatcgccc gcacgctggt tcgcatggcc gacgaagacg
     1441 ccaagcctaa cgcccagcgg ctgcccgaat tcggccaggc cggccgggct tcgctcgagc
     1501 agcagctcta ctccgaagcg ccgatctaca ccgacctgga gatcgtcaag ctggccgatt
     1561 cgctgggcat gctgatggag caactcggcg gcaaccacga actggtgaag caagtcctgg
     1621 agggtaagtc gccgcgggtg cgggcggccg agctgatcgg cgggtcgaag ctggccgacg
     1681 tggccgagcg gaagaagctt gccggcggcg gccaggcggc aatcgccgcc tccaaagacc
     1741 cgctcatcaa gctggccaag gccgtcgatc cccgctcccg cgagctgcgg acgatgttcg
     1801 agcagcagat cgaagagccg cagcggcagg cctatgccaa gatcgcggcc gcgcggttcg
     1861 ccgtgctggg cagcacgacc tatcccgacg cgacgttcac gctgcggctg gccttcggcg
     1921 cggtcaaggg ttacgaggat gaagggcgga agattgagcc ctggacgacg atgggcggca
     1981 cctttggcca cgccgaggcg cacggcagca aggacccgtt cgagctgccg ccgagctggc
     2041 tgaagaaccg cgacaagatc agcgccaaca cgccgttcaa cttcgtgagc acggccgaca
     2101 tcatcggcgg caactccggc agcccggtcg tcaacaaggc cggcgagctg gtgggcatca
     2161 tcttcgacgg caacatccag tcgctggtgc tggactacgc gtttagcgac gtccaggccc
     2221 gcgcggtgag cgtccattcc gcgtcgatcc aggaggccct ccgcaagatt tacagcgcga
     2281 ccgcgctggc cgacgagctg ggaaaataga ggccagccat gcggatcgcg gtcggcggca
     2341 tcctgcacga gaccagcacg tttctgccgc agacgacggg cgtcgaggat ttcgccgccg
     2401 gcttcggcct gttccgcgga gcggccgtgc gggagcggtt cgcgggcgca aacatgtgca
     2461 tcggcgggtt ctttgacgcc gccgagtcgt gcggctttga cgccgtgccg ctcctctggg
     2521 gctttgccta tcccagcggc ctgatccgca acgcggatta tggccgcctc aaggcggagt
     2581 tcctcgagcg gctggcagca gccgatgccg agcggcggct ggacggcgtg ttgctcgacc
     2641 tgcatggcgc gatggtcatc gagggcctgg aggacggcga cggcgacgtg gtcgccgcgg
     2701 tccgcgaagc gatcggcacg gagcggccga tcgcagtgac gtacgacctg cacggcaacc
     2761 acacgccgcg gcgaatgcaa ctggccaatg ccagcatcgg ctacgacacg tatccccacg
     2821 tcgatatggc cgagcgcggc cgcgaagcgg gccagctgat ggcgcgaacg gtccgcggcg
     2881 aagcccgccc agtcaccgcg ctcaagcaga tacccatgtt ctggagcgct cagcggcaag
     2941 tcaccgccca cgagccgatg gacgaagtcc tgcggcggat tcacgagctg ga
//
LOCUS       DASRJB010000551         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3536450, whole genome shotgun sequence.
ACCESSION   DASRJB010000551 DASRJB010000000
VERSION     DASRJB010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536450"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFB80_14320"
     CDS             <1..211
                     /locus_tag="VFB80_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN34999.1"
                     /translation="LPGAKGTTHGSPWHYDTHVPLLLVGAGIAPGQFDRPVGPACLAS
                     TVAELFGVDYPSGNVEQPLREALVK"
     gene            281..505
                     /locus_tag="VFB80_14325"
     CDS             281..505
                     /locus_tag="VFB80_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35000.1"
                     /translation="MTIDLDAATRQRLDDLARSQGQDAPALARRILLDYLDFQALPAD
                     SAEDWAAASVALAPEAMDPSDWNDSSHDAQ"
     gene            492..806
                     /locus_tag="VFB80_14330"
     CDS             492..806
                     /locus_tag="VFB80_14330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZN35001.1"
                     /translation="MTRSEVWWADLPPPLGRRPVLILTRSTAVAARNQVVVAQVTTTV
                     HGLPCEVSLTRADGLPRDCVVNCDVLLTVPKARQTSRIARLSRPKMDAVHQALKFALD
                     IP"
     gene            827..2530
                     /locus_tag="VFB80_14335"
     CDS             827..2530
                     /locus_tag="VFB80_14335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN35002.1"
                     /translation="MPTTLGFWYERDGYDTGGQRLLGRQAAGEAFLRSLLKHTAAERL
                     YCVAHNREQHKDCVARAQSWQVNRELAFLPADDPARLAEAGIVYRPDPGLPDLAWLRR
                     FGDQRAYSLCGVTHTICTKEVMRLFGQLAIAPVQPWDALICTSTAVKVAVERTLAEWT
                     EYLALRCGGKPAVPLQLPVIPLGIEAASFAASKETQSIRAGLRKTLDIAENDLVLLYV
                     GRLNFYAKAHPIPMYLAVERAFKAATSGRGPGVGSRGSGVGKLHLLQVGWFEQEREEK
                     AFKQAAKMFCPSVPCVFLDGRRPEIRRQAWAAADLFVSLSDNIQETFGLTPLEAMAAG
                     LPVVCTDWDGYQETVRHEIDGFRIPVTLPPAGSGVELARDYLSDRLMYNAYIGRACQS
                     TAADIDAVTRAIVALIEQPALRAKMGAAGRERAVGEFDWPVIIRRYEALWDELAELRS
                     RATETAPLTAGVPAHPLCDDPFRAFGHYSERHLHPAMVLALGESGTVTQFTLLLKDWM
                     GSFGGDRRLTQPEFTALLDHLKNIGPAPLAEFLRIYPGREAEVYRTIGWLLKFDVVQA
                     K"
     gene            2617..>3951
                     /locus_tag="VFB80_14340"
     CDS             2617..>3951
                     /locus_tag="VFB80_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN35003.1"
                     /translation="MVFRLRTCLLIVVLAAVSSSTVSFAADPAWKEVAIPDEWKKAPT
                     GENGWLWYRAKVRLPATWRDRKFELAVEAIDDAREIFFNGQSLGKLGQFPPQYRSGLG
                     ETLRLAIPPAAVKHAADNVVAIRVCNIDGRNGFNVAAPVLFAGQAAIRMAGKWETANG
                     DDPAWALVQAAAIKTEPFGKVEDAATIEKELKKLASGDDGPLSPAESLARMKTPDDLQ
                     VELVLSEPQIGQPLSMKWDGRGRLWVVNYLQYPNPAGLKMISRDKHLRTVYDQVPPAP
                     PHHFRGADKITIHEDTDGDGRLDRHKTFLEGSSLLTSFEFGRGGVWVLNPPYLLFYPD
                     ADGDDVPDGDPVVHLEGFGIEDSHSVTNNLRFGPDGWLYATQGSTVTGNIKRPGDKTV
                     VHSEGQLVWRYHPELKKYEIFAEGGGNAFGLEIDSKGRIYSGHNGGNTRGFHY"
BASE COUNT          685 a         1329 c         1282 g          655 t
ORIGIN      
        1 cctgcccggg gccaagggca ccacccacgg cagcccctgg cactacgaca cgcacgtgcc
       61 gctcctgctg gtcggcgcgg gcatcgcccc gggccaattc gaccgccccg tcggccccgc
      121 ttgcctggcc tccaccgttg ccgagctgtt tggcgtggat tatcccagcg gcaacgtcga
      181 gcagccactc cgcgaggcgc tggtgaaatg aacgtggtcg ggtggcaacc ggcgtagtac
      241 acttgactag gagaacgtac gtggtgcttc catgccccaa atgaccatag acctcgacgc
      301 cgctactcgg cagcgactcg acgatctagc gcggtcgcag gggcaggacg cccccgcttt
      361 ggcccgtcgc attctgctcg actatctcga ttttcaagcg ctgccggccg actcggctga
      421 agactgggcg gccgcctctg tggctttagc gccggaggcg atggacccat ccgactggaa
      481 cgactccagc catgacgcgc agtgaagtct ggtgggccga cctgccgcct ccgcttggcc
      541 gccgcccggt cctcattctc acgcgcagca cggcagtcgc cgcgcgcaat caggtcgtcg
      601 tcgcacaggt caccacgacc gtgcatggac tgccctgcga ggtcagcttg acccgggccg
      661 acggtctccc gcgagattgt gtcgtgaatt gtgacgtctt gctcacggtg cccaaggccc
      721 gccagacttc gcgtatcgcc cggctctcgc gtccaaagat ggatgcagtg catcaagcgc
      781 tgaagttcgc cttggacata ccgtgaagtg caattgagag cgatccatgc ccaccacgct
      841 cggcttctgg tacgaacgcg acgggtacga caccggcggc cagcggctgc tggggcggca
      901 ggcggcgggc gaggcgttct tgcggtcgct gctcaagcac acggcggccg agcggctgta
      961 ctgcgtggcg cataaccgcg agcagcacaa ggactgcgtc gcgcgggcgc agtcgtggca
     1021 ggtgaaccgc gagctggcat ttctgcccgc ggacgatccg gcgcgtctgg ccgaggcggg
     1081 catcgtctat cgccccgatc cggggctgcc cgacctggcc tggctgcggc ggttcggcga
     1141 ccagcgggcc tacagcctgt gcggagtcac ccacaccatc tgcaccaagg aagtgatgcg
     1201 gctgttcgga cagctggcga tcgcgcccgt gcagccgtgg gatgcgctga tttgcacctc
     1261 gaccgccgtg aaggtcgcgg tcgagcggac gctggcggaa tggactgaat acctggcgct
     1321 gcggtgcggc ggcaagcctg ctgtcccgct ccagttgccg gtgattccgc tgggaatcga
     1381 agcggcgtcg tttgccgcga gcaaggaaac gcaatcgatc cgcgccggct tgcgaaaaac
     1441 gctcgacatc gccgaaaacg acctcgtcct cctctacgtc ggccggctga atttctacgc
     1501 caaggcccac ccgattccga tgtacctggc cgttgagcgg gccttcaaag ccgcgaccag
     1561 tggtcgcggg ccgggagtcg ggagtcgggg atcgggagtc ggaaagctcc acctgctcca
     1621 agtcggctgg ttcgagcagg aacgggaaga gaaggcgttc aagcaggcgg cgaaaatgtt
     1681 ctgccccagc gtgccgtgcg tcttcctcga cggccggcgg ccggaaatcc gccggcaggc
     1741 ctgggccgcg gccgacctct tcgtgtcgct gtccgacaac atccaggaaa cgttcggcct
     1801 cacgccgctg gaggcgatgg ccgccggcct gcctgtcgtc tgcaccgact gggacggcta
     1861 ccaggaaacc gttcgccacg agatcgacgg cttccgcatc ccggtcaccc tgccacccgc
     1921 cggcagcggc gtcgagctgg ctcgcgacta cttgagcgac cggctgatgt acaacgccta
     1981 tatcggcagg gcctgccaga gcacggccgc cgacattgac gccgtcacgc gagcgatcgt
     2041 cgcgcttatc gagcagccgg cactgcgggc gaagatgggg gctgccggcc gcgagcgagc
     2101 cgtgggcgag ttcgactggc cggtcatcat ccgccgctac gaagccctgt gggacgagct
     2161 ggccgagctc cgctcgcgtg cgaccgagac cgcgccgcta actgccggcg tgcccgccca
     2221 tccgctctgc gacgatccgt ttcgcgcgtt cggccactac agcgagcggc acctgcaccc
     2281 ggcgatggtg ctcgcgctgg gcgaatcggg cacggtgacc cagttcaccc tgctgctcaa
     2341 ggactggatg ggatcgttcg gtggcgaccg tcgcctgacg cagcccgagt tcaccgcgct
     2401 gctcgaccac ctcaagaaca tcggcccggc gccgctcgct gaattcctcc gcatctatcc
     2461 cggccgcgaa gccgaagtct atcgcaccat cggctggttg ctgaaattcg acgtcgtgca
     2521 ggcaaagtag taggcacact ccgtgtgccg tcgtaccttc gacgagttat catcaagcat
     2581 cccgccgcgt gccccgccta tacgcccgag atatctatgg tctttcgcct gcgaacttgc
     2641 ctactaatcg tcgtcctagc ggctgtttcg tcgtcaaccg tcagctttgc cgccgatcct
     2701 gcctggaaag aagtcgccat tcccgacgag tggaaaaagg ccccgaccgg cgaaaacggc
     2761 tggctctggt accgcgcaaa ggtgcggctg ccggcgacct ggcgggaccg gaagtttgag
     2821 ctggccgtgg aggcgatcga cgacgcccgc gaaatctttt tcaacggcca atcgctcggc
     2881 aagctgggtc aattcccgcc gcagtatcgc agcggcctgg gcgaaacctt gcgcctggcg
     2941 attccgccgg cggcggtcaa gcacgccgcg gacaacgtcg tcgcgatccg ggtctgcaat
     3001 attgacggcc gcaacggctt caacgtcgcc gcgccggtgc tgttcgcggg gcaggctgcc
     3061 atccgaatgg ccggcaaatg ggaaacggcc aatggtgacg atccggcctg ggctttggtc
     3121 caggccgctg cgatcaaaac ggagccattc ggcaaagtcg aagacgccgc cacgatcgaa
     3181 aaggagctga agaagctggc ctcgggcgac gatggcccgc tctcgccggc cgagtcgctg
     3241 gcccgcatga aaacgcccga cgacctgcag gtcgagctcg tgctctccga gccgcagatc
     3301 gggcagccgc tgtcgatgaa atgggacggc cgcggccgct tgtgggtcgt gaactacctg
     3361 cagtatccca acccggccgg cctgaagatg atcagccgcg acaagcatct gcggactgtc
     3421 tatgaccagg tgccccccgc gccgccgcac cattttcgcg gggccgacaa aattacgatc
     3481 cacgaagaca ccgatggcga cggccggctc gaccggcaca agacgtttct cgaaggctcg
     3541 agcctgctga cctcgttcga gttcggccgg ggcggtgtgt gggtgctcaa cccgccgtat
     3601 ctgctcttct atcccgacgc cgatggcgac gacgtgcccg acggcgaccc ggtcgtgcac
     3661 ctggaaggtt ttgggatcga ggactcgcac agcgtgacca ataacctccg cttcgggccc
     3721 gacggatggc tgtatgccac gcagggcagc accgtgacgg gcaacatcaa gcggccgggc
     3781 gacaagacgg tcgtgcactc cgaggggcag ctcgtctggc ggtatcaccc tgagctgaag
     3841 aagtatgaga ttttcgccga aggcggcggc aacgccttcg gcctggagat cgacagcaag
     3901 ggccgcatct acagcggcca caacggcggc aacactcgcg gcttccacta c
//
LOCUS       DASRJB010000552         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1280400, whole genome shotgun sequence.
ACCESSION   DASRJB010000552 DASRJB010000000
VERSION     DASRJB010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280400"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(711..1466)
                     /locus_tag="VFB80_14345"
     CDS             complement(711..1466)
                     /locus_tag="VFB80_14345"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HZN35004.1"
                     /translation="MIWSQRWLDCLFLHFPVPPAALSRLVPPRLEVEAFAGSAWISYV
                     LFRLKLRPAWLPLVPGFSSLVELNLRTYVRHRGQSGICFLRIFADNRLSIAAARLLTP
                     LPYEHARMAYGSRSASCLPTADPGRRLLVQFRSPTSFSKMKPGTLDAWLLERYRLFVG
                     GQNGSVLAADAEHSPWQAASLAPVVIENSLAADLSQDLGTHPAAAHYSPGVAARFHTF
                     RTVAAQLADPGRRHEPSSPGSVVFHRVFRSRGE"
     gene            1650..2441
                     /gene="nagB"
                     /locus_tag="VFB80_14350"
     CDS             1650..2441
                     /gene="nagB"
                     /locus_tag="VFB80_14350"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZN35005.1"
                     /translation="MRVIIQPDADSASRRAARFVADLIRKKPQCVLGLATGSSPLGTY
                     RELIRLHKEEGLDFSQVTTFNLDEYVGLGPAHPQSYRAFMQQNLFDQINIDPKRTNVP
                     DGRALDFENHCRQYEQRIKDAGGIDLQILGIGSDGHIAFNEPGSSLGSRTRLKTLTSE
                     TIRDNARFFGGEDKVPRLAVTMGVGTILESRRCLLLAFGPHKSLAIRDTVEGPVTAQV
                     TASALQLHREVIGIFDEAAARLLVRRDYYAEVERAQSQLEAGQKP"
     gene            2482..2898
                     /locus_tag="VFB80_14355"
     CDS             2482..2898
                     /locus_tag="VFB80_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZN35006.1"
                     /translation="MARIERFEDIEAWKRARQLTKKIYDCTRAPRFARDFALKDQICR
                     ASVSTMSNMAEGFERGGNQELIQFLATAKGSIGEVRSQLYVALDQGYITQQQFDEMYG
                     DCSAISRMIAGFISYLQKSAHRGSKFVRPRRASSRP"
     gene            2931..3923
                     /locus_tag="VFB80_14360"
     CDS             2931..3923
                     /locus_tag="VFB80_14360"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN35007.1"
                     /translation="MRQLGIITDEREAERLAAWLVAQRIEAHAEEAGEAGFAIWVRDE
                     DQLPQARAALEHFRANPQDPRYQNAERDAAEFQRKEEAKRRDAQKNVVQMRSRWGSGL
                     PGSGMGGPPRRAPCVLTLIGLSVLLGIFTYSDTLGFDEGATERPPPSGIYRGMLLADP
                     ETAQSIWDNIARGQVWRTITPIFIHFGWPHLVFNMICLYSFGSQIEDRKGTRFMLLLV
                     LVLAVTSNLGQAAEYAWRQVGGEFGGMSGVAYGLFGYLFVKTRYDNRSGFHLSPVTSF
                     MAILWFVLCIARDVPPFSALLQNAIPAIANSAHAVGLIVGAALAYAPLVLRKPA"
     gene            4004..4240
                     /locus_tag="VFB80_14365"
     CDS             4004..4240
                     /locus_tag="VFB80_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35008.1"
                     /translation="MPLFHFGSRFALTSLFGAIAWLAAEQPLAAQVLLAQSGAAKSIL
                     GWLLTLLAIALGLIVVCRPSSRKDPDKKEKEPRK"
     gene            complement(4276..4635)
                     /locus_tag="VFB80_14370"
     CDS             complement(4276..4635)
                     /locus_tag="VFB80_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN35009.1"
                     /translation="MNAHEFYAIDTRQQPWQERFNEKIGRALFRKDLYLDPVSGAEIR
                     LVRYPAGVINPRHTHPCGHGMYVLEGQLVTHKGTFGPGHFVWFPEGEVMEHGASAAGD
                     VTVLFITNKAFRMDYLP"
     gene            4711..>6143
                     /locus_tag="VFB80_14375"
     CDS             4711..>6143
                     /locus_tag="VFB80_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN35010.1"
                     /translation="MPILNDRKAALLISAACLLWVAAAGAAEPYDIVIRGGRIVDGTG
                     APWYVADIGIRGGKIAKIGRLNAADGKRLIDAAGLIVAPGFIDMMGQTATPMLEDPAS
                     AINLLTQGITTINAGEGASAAPLDDEAAKKAGWRTMGEYFRRIDERGLPLNVAQTIGH
                     TQVRKIVLGDVDRRPSDAELDQMRALVKEAMEAGAIGVSTALIYPPAVYATTEEVAAL
                     AEVAGQFGGRYYTHMRNEGDRLEEAIDEALEIGRRAKTPVHIFHLKTAGRQNWGKMPL
                     AIAKIKAARTAGQQVTADIYPYINNGLGIEAFIHPRHAAEGHQRLIRKLDDPAVRAEI
                     RKEMETTSGWENWYRHVGHDWSLVVIGRANGMRYSALAGQSVAAMAQARSEDPWDTFF
                     NLVSEGAFALPQSMSEANKIAAIQQEFVSFCTDVGPAGGSLIAGHPRAFGAFPRLLSR
                     YVRDQGVISLERAVAQASAAGANNVLA"
BASE COUNT         1205 a         1906 c         1965 g         1067 t
ORIGIN      
        1 cgcctcgcag aagcaagtcg tcaacgtcgc ccaacgcggc ggcatgtgcg tcgtcttcat
       61 cggccgccac agcggacggt tgcatggacc agggtgagtc gcccgcttaa ttgccagttc
      121 cgcagtctag aaaagtcgat aattctggaa ttatgtcgga ataattgcaa gcctttgtgc
      181 ccaagcaggt tgcggcggca ctttcgcgga tcgaccgccg aatcgcgcct gtattcaccg
      241 aaccatattg tataatcccg ttcacatagc ggcggtttcc agcgatgaac aagccctggg
      301 aacaacaagg ctgtattgcc tgccgaattg cagccgcggg cggaatgcgg tcgccgctca
      361 tcttgattga caatgacgat gaattgaccc agttcgcgcg gctttggcga tgcgagaagt
      421 gccaggcgtt ctgggtcgag gaacaacgct ctgcttgtga gatttcggag agcgcagcac
      481 aaaagagatt tccgagcgta tttggtccac atgcttgacc acattcagaa catgtggaga
      541 taaccgattt tttcaccaca tgggttttgc ccgaaacagt ggcggccaaa ggagttgcga
      601 agagtactgc cgattaattc accggttgcc caggtatcgc gctgttttgg cgatttaatc
      661 gcaccgccaa gtgcagcaaa acctgagaaa aacggtcaaa gggtttggaa ttattcacca
      721 cgtgatcgaa agacccggtg aaaaacgacc gagcctggcg atgacggttc atggcgccgg
      781 ccggggtcag ccagttgagc cgctacagtg cgaaatgtat ggaaccgcgc tgcgaccccg
      841 ggcgagtaat gcgcggctgc gggatgcgtt ccgaggtcct gactcaagtc ggcggcgagc
      901 gagttttcga taacgactgg cgcgagcgac gcggcctgcc acggcgagtg ctcggcgtcg
      961 gcggcgagga cggagccgtt ctggccgccg acgaacagcc ggtagcgttc cagcagccag
     1021 gcgtcgagcg tgcccggttt catcttggag aatgaagttg gtgatctgaa ttgcaccaac
     1081 agccgtcgcc cgggatccgc tgtcggcagg caacttgccg agcgcgaacc gtaggccatt
     1141 cgtgcgtgct cgtagggcaa tggtgtcagc agtctggcgg ccgcaatcga aagtcgattg
     1201 tcggcgaaga tgcggagaaa gcaaatgccg ctctggccgc ggtggcggac gtaagtgcgg
     1261 agattcaatt cgaccagcga cgagaatccc ggcaccagcg gcagccacgc cggccgcagc
     1321 ttcagccgga acagcacgta gctgatccag gccgatcccg cgaaggcttc gacctccagc
     1381 cttggcggca cgagcctcga cagcgccgcc ggcggcacag gaaagtgcag gaacagacag
     1441 tccagccagc gctgcgacca gatcatggca gcggcctccc ggctgccaac cgcagtatgg
     1501 ttggagcatt aaccgctggc cgcggccttg ttggcggaaa gcgggcgttg gtatgctgcg
     1561 agcggtcagc tgtcattggt cattcatttg tcatttgtga gtagtgaatc gacctgcgct
     1621 gtcctgaacc ccgaaccctg aaccctgccg tgcgcgtcat cattcagccc gatgcggatt
     1681 ccgccagccg gcgggctgcg cggtttgtgg cggacctgat ccgcaagaag ccgcagtgcg
     1741 tgttgggcct ggcgacgggc agcagcccgc tgggcaccta tcgcgagtta attcgcctgc
     1801 acaaggagga ggggctcgat ttctcgcagg tcacgacctt caacctcgac gaatacgtcg
     1861 gccttggtcc ggcacatccg cagagctacc gggcgttcat gcagcagaac ctgttcgacc
     1921 agatcaacat cgaccccaaa cggacaaatg tgcccgacgg ccgggccctc gatttcgaga
     1981 accattgccg gcagtacgag cagcggatca aggacgccgg cggcatcgac ctgcagattc
     2041 tcggcatcgg ctccgacgga catatcgcgt tcaacgagcc gggctcgtcg ctgggcagcc
     2101 gcacgcgctt gaaaacgctg accagcgaga cgatccgcga taacgcccgc ttcttcggcg
     2161 gcgaggacaa ggtgccgcgg ctggcggtca cgatgggcgt cggaacgatc ctcgaatcgc
     2221 ggcgctgcct gctgttggcg tttggccctc acaagtcgct ggccatccgc gacaccgtcg
     2281 agggccccgt caccgcgcaa gtcaccgcat cggccctaca attgcaccgc gaggtcatcg
     2341 gcattttcga cgaagccgcc gcccggctgc tcgtgcggcg ggactactac gccgaagtcg
     2401 aacgcgctca gtcgcaactc gaagcggggc agaagcccta gttcaaggtt caaagttcaa
     2461 ggttaaggtc gaggatgagt cttggcgaga attgaacgat tcgaagacat cgaagcctgg
     2521 aaacgcgcta ggcaactcac gaagaagatc tacgattgta caagggcgcc caggttcgcc
     2581 cgcgacttcg cactcaagga tcaaatctgt cgcgcttccg tttcgacaat gagcaacatg
     2641 gccgaaggct tcgagcgcgg cggaaatcag gagctcatcc agtttctggc aacggcgaaa
     2701 ggctcgatcg gcgaagttcg gtctcaactc tacgtcgcgt tagaccaggg ctacatcacg
     2761 cagcagcagt tcgacgaaat gtacggcgac tgcagcgcga ttagtcgcat gatcgcaggc
     2821 ttcatcagct accttcagaa atcggcacat cgcggcagca agttcgtccg accgaggcgc
     2881 gcgtcttccc gaccttgaac tttgaacctt gaacttgaac ctttgagata atgcgtcaac
     2941 tcggaatcat cacagacgag cgcgaggccg agcgcctggc tgcctggctg gtggcgcagc
     3001 ggatcgaagc gcatgccgag gaggcgggcg aggcgggctt tgcgatctgg gttcgcgacg
     3061 aggaccagtt gccgcaggcc cgcgcggcct tggaacattt tcgggccaat ccgcaggatc
     3121 cgcggtacca gaacgccgag cgcgacgcgg ccgaatttca gcgaaaggaa gaagccaagc
     3181 gccgcgacgc gcagaagaac gtcgtccaga tgcgcagccg ctggggaagt ggcctgccgg
     3241 gctccgggat gggcggcccg ccgcgccgcg ctccctgcgt gctgacgctg atcggcctgt
     3301 cggtcctgct gggcatcttc acgtacagcg acacgctcgg tttcgacgaa ggagcgaccg
     3361 agcggccgcc accgagtggc atctatcgag gaatgctgct ggccgatccg gaaacggccc
     3421 aatcgatctg ggacaacatc gcccgcggcc aggtctggcg cacgatcacg ccgatcttca
     3481 ttcacttcgg ctggccgcac ctggtcttca atatgatctg cctctacagc tttggctcgc
     3541 agatcgagga ccgcaaaggg acgcggttca tgctgctcct cgtgctggtg ctggccgtga
     3601 cttcgaatct cggccaggcg gccgagtatg cctggcggca agtcggcggc gagttcggcg
     3661 gcatgtcggg cgtggcgtat gggctgttcg gttacctgtt cgtcaaaacg cggtacgaca
     3721 accgctcggg gtttcacctc agccccgtca catcgttcat ggcaattctc tggttcgtct
     3781 tgtgcatcgc ccgcgacgtg cccccgtttt cggcgctgct gcaaaacgcc atcccggcga
     3841 tcgccaactc cgcccacgcg gtgggcctga tcgtcggcgc ggcgctggcc tatgctcctt
     3901 tggtcctgcg caagccagcc tagcgagaaa ctgctacaat ctccgctcga ctcgaattag
     3961 aattcagcca ccagcaacca cactgcctcg ccgctgccgc ccgatgccac tcttccactt
     4021 cggatctcga tttgcattga ccagcctgtt cggcgctata gcctggctgg ccgccgagca
     4081 gccgctcgcg gcccaggtgc tgctggctca aagcggggcg gccaagagca ttctcggctg
     4141 gctgctgacg ctgctggcga tcgccttggg gctgatcgtc gtctgccggc cgagcagccg
     4201 caaggatccg gacaagaagg agaaggagcc gcgcaagtag cggcggcttt cgcggcccga
     4261 ggatcgcagc gcttgctagg gcagatagtc catccggaag gccttgttgg tgatgaagag
     4321 cacggtgacg tcgccggccg cgctagcgcc gtgctccatg acctcgccct cggggaacca
     4381 aacaaagtgc ccggggccga acgtgccctt gtgcgtgacg agctgcccct cgaggacgta
     4441 catgccgtgc ccgcaggggt gcgtatgccg cggattgatg acgccggccg gatagcggac
     4501 caggcgaatc tccgcgccgc tgaccgggtc gaggtacagg tctttgcgga acagggcccg
     4561 gccgattttc tcgttgaacc gctcctgcca gggctgctgc cgcgtatcga tcgcatagaa
     4621 ttcgtgggcg ttcatgggac ttgattggta gttggggtta gcgaacgcaa aatgatcaag
     4681 caccaagatc aaatcacaaa caaatcccaa atgccaattc tcaatgaccg aaaagcggcc
     4741 ctgctgatca gcgccgcctg cttgctgtgg gtggcggccg ccggcgccgc ggagccgtat
     4801 gacatcgtga tccgcggcgg acggatcgtc gatggcacgg gagcgccgtg gtatgtggcc
     4861 gatattggca ttcggggcgg caagatcgcc aagatcggac ggctgaacgc ggccgacggc
     4921 aagcgcctga tcgatgccgc gggcctgatc gtcgcgccgg gcttcatcga catgatgggc
     4981 cagaccgcga cgccgatgct ggaagacccg gccagcgcga tcaacctgct cacgcagggc
     5041 atcacgacga tcaacgccgg cgagggcgcc agcgccgcac cgctcgacga tgaggcggcg
     5101 aaaaaggccg gttggcggac aatgggcgag tatttccggc ggatcgacga gcggggactg
     5161 ccgctgaacg tcgcgcagac gatcggccac acgcaagttc gaaagatcgt gctgggcgac
     5221 gtcgatcgcc gcccgtcgga cgcggagctc gaccagatgc gggcgctggt caaggaggca
     5281 atggaggccg gcgcgatcgg tgtttccacg gccctcatct atccgccggc cgtctatgct
     5341 acgaccgaag aggtcgccgc cctcgcagaa gtcgccgggc agttcggcgg ccgctactac
     5401 acgcacatgc ggaacgaagg cgaccggctc gaggaggcga tcgacgaggc cctcgagatc
     5461 ggccgccggg cgaagacgcc ggttcacatt ttccacctca agacggcggg ccggcagaac
     5521 tggggcaaga tgccgctggc catcgccaag atcaaagcgg cccggaccgc cggccagcag
     5581 gtcaccgccg acatctaccc ctacatcaac aacggcctgg gaatcgaggc cttcattcac
     5641 ccgcggcacg cggctgaagg ccaccagcgg ctgatccgca agctcgacga ccccgccgtg
     5701 cgggccgaaa tccgcaagga gatggaaacg accagcggct gggagaactg gtatcgccac
     5761 gtcggccacg actggtcgct ggtggtgatc ggccgcgcca atggaatgcg ctactcggcc
     5821 ctcgccggcc agagcgtcgc cgccatggct caggcccgca gcgaggaccc gtgggatacg
     5881 ttcttcaacc tcgtcagcga gggcgcattc gccctgccgc aaagcatgtc cgaggccaat
     5941 aaaattgccg ccatccagca ggagttcgtc tcgttctgca ccgatgtcgg acccgcaggc
     6001 ggctcgctca tcgccggcca tccgcgggcc ttcggcgcgt tcccgcggct gctgtcgcgc
     6061 tacgtccgcg accagggcgt gatctcgctg gagcgggccg tcgcccaggc cagcgccgcg
     6121 ggcgcgaaca acgtgctggc ctt
//
LOCUS       DASRJB010000553         5445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2693892, whole genome shotgun sequence.
ACCESSION   DASRJB010000553 DASRJB010000000
VERSION     DASRJB010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693892"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFB80_14380"
     CDS             complement(<1..664)
                     /locus_tag="VFB80_14380"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN35011.1"
                     /translation="MRRRRFLQTAAAAALSAAACPSVRAAGIAARYEGLATDRYLSDL
                     KTVAKVMGPEIFTEGPCCDRAGNVYFTNTAASQILKWDGKQLSVFREDKNAANGLLID
                     PQGRLVACEGGSGRVTRTDLSTGQVTVLADQFGGHPLGAPNDLCFDRQGRIYFTSRLP
                     NTDPAKGNVNAVYRIDPDGKLSQILRAPEIDMPNGIVTSPDDKILYLVESDGHESRKR
                     CIL"
     gene            complement(674..1657)
                     /locus_tag="VFB80_14385"
     CDS             complement(674..1657)
                     /locus_tag="VFB80_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN35012.1"
                     /translation="MLIRSFALILTLIAAPLWAQAPQAAQKKAAEKKAPERPAPTAAD
                     FAYASDSPRQKFDFWQAKSDAPTPLVLLIHGGGWTGGDKTSYGTAAIQPYLDAKISVA
                     AINYRYILQAMDDKVEPPVKGCLHDAARALQTLRSKAKEWNLDPARVGSTGSSAGACT
                     SLWLALHDDLADPKSNDLVARQSSRLTCAAVSGAQTSLDPKELKEWIPNAIYGGHAFG
                     FAAKGRTRPEEFELLIANREKVLPWIKEYSPIELVSQDDPPIFLDYPNQRTEPKVGQP
                     EADPTHSAMYGVQLESKLKSAGVEVVLSYPGHKDEKYGSIQKFLIEKLTTK"
     gene            complement(1686..2231)
                     /locus_tag="VFB80_14390"
     CDS             complement(1686..2231)
                     /locus_tag="VFB80_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35013.1"
                     /translation="MSQEYLLPCSCGQQLRVAPAQAGGRVSCACGRSMSVPTLRGLRE
                     LKLAPQAKAAKAAAGWSPVHGTIFAAGMLLAAAGLAVAAYNFVFYRAYRPLTVDQTEA
                     VVRHAELQRPIDKLTPLAALEEWKTEVLPGLSKSLDPDWILARKAAARHLLWSKVGGG
                     MLAGGLLTAMATLFIGRGQKT"
     gene            complement(2311..3624)
                     /locus_tag="VFB80_14395"
     CDS             complement(2311..3624)
                     /locus_tag="VFB80_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35014.1"
                     /translation="MSARAANDSRREFLKASSAAAATAGLAATFGALPAVHAQGSGAI
                     NVALIGCGGRGTAAAMNAMQADPDNRLTMMVDAFPDRVQQSRQSLTRQLGQQMRVTDD
                     TALSGLDAYKRVMESDVKVVILTTPPHFRPAQLKAAIDAGKHVFCEKPVAVDAPGVQS
                     VLKTTEEARKKGLAIVSGLCWRYDSAVRETIKRIQDGAIGDIVAIQENYLTGTLWHRG
                     RKPEWSEMEYQIRNWLYYTWLSGDHNVEQHIHSLDKAMWLMGDKPPLAATGLGGRQVR
                     TGPEWGNIYDHHAVVYEWASGVRVFAFTRQMGGQQVFNETDDIILGTKGQARILKNEI
                     TGENPWKYPRAKKDADKGMYDIEHDELFASIKAGKPINNGVYMSYSTMLAILGRMATY
                     TGQRITWDEAMKSTLDMTPKSYAMGPVELPPGVLEVAMPGVTKLT"
     gene            complement(3732..4697)
                     /locus_tag="VFB80_14400"
     CDS             complement(3732..4697)
                     /locus_tag="VFB80_14400"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HZN35015.1"
                     /translation="MNADLQTCDVPKPPRASAEVPRWIVWSLRAAGIYNLVWGAWVIL
                     FPAAFWSWLELPQPNYPALWQCIGMVIGVYGVGYWIAARDPARHWPIVLVGWLGKVLG
                     PIGMLYAALRGNLPWSFGIVNVWNDLIWWWPFSAALYYAWRANSAPPVGAPLNFAQAV
                     GTIRSQRGRTLAELSAPSPLLVVFVRHAGCTFCREALADVAAAKQDLDRAGTALAVVH
                     MSTATRASELLEHYSLGDIDQFSDPACRLFQAFELARGNWWQLLGPPIWWRGLTAIFR
                     HGLGPIDGDGFQMPGAFLLRGGRIARAYRHRTAADRPDYVKLAAA"
     gene            complement(4921..>5445)
                     /locus_tag="VFB80_14405"
     CDS             complement(4921..>5445)
                     /locus_tag="VFB80_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35016.1"
                     /translation="AVAGVAGAIGYRVALRRRRDRLRAGQLLLSVMRFPITGNRRKWT
                     VWGLTAAVILDGVVMSVLVLPAAPLFDSQDDFPPMLGFAVQVVLVQAILWCACLDGWT
                     YDVALGFHEHGLLTKERFYAWTTISRIQWTSPQLAVYGEHSLIVGIDPADFQSVTGVL
                     EIIRQRTGVPITSE"
BASE COUNT          932 a         1800 c         1769 g          944 t
ORIGIN      
        1 ccaggatgca ccgcttacgg ctctcgtggc cgtcggactc gacgaggtac agaatcttgt
       61 cgtcgggcga cgtgacgatg ccgttgggca tgtcgatctc cggagcgcgg agaatctgcg
      121 acagcttgcc gtccggatcg atccgataca cggcgttgac gtttcccttg gccgggtcgg
      181 tgttgggaag ccgcgacgtg aagtagatcc gcccttgcct gtcgaagcac aaatcgttgg
      241 gcgcgcccag cggatggccg ccgaactgat cggcgagcac agtcacttgc cccgtgctca
      301 gatcggtccg cgtgacgcgg cccgaacccc cctcgcaggc caccagccgg ccttgtggat
      361 cgatcagcag gccgttagcg gcgttcttat cctcgcggaa tacggagagt tgcttgccgt
      421 cccacttgag aatctgcgag gcggccgtgt tcgtgaaata gacgtttccc gcgcggtcgc
      481 agcagggtcc ctcggtgaaa atctccggtc ccatcacttt cgcgacggtc ttcaggtcgc
      541 tcaggtatcg atccgtcgcc aaaccctcgt atcgcgccgc gattcccgcc gcgcggacgg
      601 aaggacaagc cgccgcggaa agggccgcgg cggctgcagt ctgaaggaat cggcgacggc
      661 gcatcggacg ctcctacttg gtcgtcagct tctcgatcag gaacttttgg atcgagccgt
      721 acttctcgtc cttgtggccg ggatatgaca gcacgacctc gacgcccgcg ctcttcagct
      781 tgctttccag ctgcacgccg tacatggccg agtgcgtggg atcggcttcc ggctggccga
      841 cttttggctc ggtccgctgg ttcggatagt ccagaaagat cggcgggtcg tcctggctga
      901 ccagctcgat cggcgaatac tccttgatcc agggcagtac cttctcgcga ttggcgatca
      961 gcagctcgaa ctcctcgggc cgcgtgcgcc ccttggcggc gaagccgaac gcgtgaccgc
     1021 catagatggc gttgggaatc cactccttca gctccttcgg atcgagcgag gtctgcgccc
     1081 cgctcaccgc agcgcaggtc agccgcgagg actgccgcgc gaccagatcg ttgctcttgg
     1141 gatcggccag gtcgtcgtgc agggccagcc acagcgaggt gcacgcgccg gcggagctgc
     1201 ccgtggaacc aactcgcgcc ggatcgaggt tccactcctt ggccttggag cggagcgtct
     1261 gcagggcccg agcggcatcg tgcaggcagc ccttcaccgg cggctcgacc ttgtcgtcca
     1321 tcgcctggag gatgtagcgg tagttgatcg ccgccacgga aatcttggcg tccaggtaag
     1381 gctgaatcgc agcagtgccg tagctggtct tgtcgccgcc cgtccagccg ccgccgtgaa
     1441 tcagcagcac gagcggcgtc ggggcgtcgg acttggcctg ccagaagtcg aacttctgcc
     1501 gcggcgaatc gctggcataa gcgaagtcgg cggccgtcgg cgcgggccgc tccggggctt
     1561 tcttttccgc cgccttcttc tgggcggcct gcggcgcctg tgcccacagc ggagcggcga
     1621 tcaatgtcag aatcaaggcg aacgagcgga tcagcatgat aggactccca aggttgcagg
     1681 tcgcttcacg ttttctggcc acgaccgatg aagagcgtgg ccatcgcggt gagcagtccg
     1741 cccgccagca tgccgccgcc gactttggac cacagcagat gcctggcggc cgccttgcga
     1801 gccaggatcc agtctgggtc caagctcttg ctcaaaccgg gcaatacctc cgtcttccat
     1861 tcttcgagcg ccgcgagcgg cgtcagcttg tcgatgggtc tttggagttc cgcgtggcga
     1921 accacggcct cggtttgatc gaccgtcagc gggcggtagg ccctgtagaa cacgaagtta
     1981 taggcggcaa ccgccaggcc cgcggcggcc agcagcatgc cggcggcgaa gatcgtcccg
     2041 tgaacggggc tccagccggc agcggcctta gccgccttgg cctgcggtgc gagcttcagc
     2101 tcgcgcaggc cgcggagcgt cggtacgctc atgctccggc cacaggcgca gctcacccgc
     2161 cccccggcct gggccggggc gacgcggagc tgctgtccgc acgagcacgg gagcaagtat
     2221 tcctgagaca tgcggctagt acgcaaccag ccaagaacca gttcgacttt ggttcttggg
     2281 cgcttcggtt ctttagttcc tcggttttcc tcaggtcagc ttcgtcaccc ccggcatcgc
     2341 gacttcgagc acgcccgggg gcagctcgac gggccccatc gcgtacgact tgggcgtcat
     2401 gtcgagcgtc gacttcatcg cctcgtccca ggtgattcgc tggccggtgt acgtcgccat
     2461 gcggccgagg atcgccagca tcgtgctgta cgacatgtag acgccgttgt tgatcggctt
     2521 gccggccttg atcgaggcaa acagttcgtc gtgctcgatg tcgtacatcc ccttgtcggc
     2581 gtccttcttg gcccgcggat acttccaggg gttttcgccg gtgatctcat ttttgagaat
     2641 ccgggcctgc cccttggtgc ccaggataat gtcgtcggtc tcgttgaaca cctgctggcc
     2701 gcccatctgt cgcgtgaagg caaacacgcg gacgccactg gcccattcat acacgaccgc
     2761 gtgatggtcg tagatgttgc cccactcggg ccccgtgcgg acctgccgcc cgcccaggcc
     2821 cgtcgcggcc agcggcggct tgtcgcccat cagccacatc gccttgtcga ggctgtggat
     2881 gtgctgctcg acgttgtggt cgcccgagag ccacgtgtaa tagagccagt tgcggatctg
     2941 gtactccatc tcgctccact cgggcttgcg gccgcggtgc cagagcgtgc cggtgagata
     3001 attctcctgg atagcgacga tgtcgccgat cgcgccgtcc tgaatccgct tgatcgtctc
     3061 gcggacggcg ctgtcgtaac gccagcacag gccggagacg atcgccaggc ctttcttccg
     3121 cgcttcctcc gtcgtcttca gcacgctttg cacgccgggc gcatcgaccg cgacgggctt
     3181 ctcacaaaag acgtgcttgc cggcgtcgat cgccgccttc agttgcgcgg ggcggaagtg
     3241 cggcggcgtc gtgaggatca caaccttcac gtcgctctcc atcactcgct tgtaggcatc
     3301 gaggccggac agcgccgtat cgtcggtgac ccgcatctgc tggccaagct gccgtgtgag
     3361 cgactgccgc gactgctgaa cccggtcggg aaaggcgtcc accatcatcg tcaagcggtt
     3421 gtccgggtcg gcctgcatgg cgttcatcgc ggccgccgtg ccgcggccgc cgcagccgat
     3481 caaggccacg ttgattgcgc cgctcccctg ggcgtgtacg gcgggaagag cgccaaaggt
     3541 cgcggccagg ccggccgtcg cggctgctgc agacgaagcc ttcaggaatt cgcggcggga
     3601 atcgttggcg gcacgagcgg acatcggcgg gctccaggaa gaatggttca ggcgggacgc
     3661 ggggggtgcc gcgcggcggt aaggccaacc gccaatctta attgccagcc gccgcatctg
     3721 acaaccggcc gctatgccgc cgccagtttg acgtaatccg gccgatcggc ggcggtccga
     3781 tggcgatagg cgcgggcgat ccgcccgccg cgcagcagaa aggcgccggg catctgaaag
     3841 ccgtcgccgt cgatcggtcc caagccgtga cgaaagatgg cggtaagtcc gcgccaccaa
     3901 atgggcgggc cgaggagctg ccaccagttg ccgcgggcca gctcgaaggc ctggaacagc
     3961 cggcaggccg gatcgctgaa ctggtcgatg tcccccaggc tgtagtgctc cagcaattcg
     4021 ctggcgcggg tcgccgtgct catatggacg acggcgagcg cggtgccggc gcgatcgagg
     4081 tcctgcttgg ctgccgcgac atcggccagc gcctcgcggc agaaggtgca gccggcatgg
     4141 cggacaaaga cgaccagcaa cggcgagggc gccgagagct cggccagggt acggccgcgc
     4201 tggctgcgaa tcgtaccgac ggcctgggcg aagttgagcg gcgcgcccac tggcggggcg
     4261 gagttcgccc gccaggcgta gtagagcgcc gcgctgaacg gccaccacca gatgaggtca
     4321 ttccagacat tcacaattcc gaacgaccac ggcaggttgc cgcggagggc ggcatagagc
     4381 atgccgatcg gaccgagcac cttgccgagc cagcccacaa ggacgatcgg ccaatggcgg
     4441 gccggatcgc gggcggcgat ccaatagccg acgccgtaca cgccgatgac catgccgatg
     4501 cactgccaga gcgcgggata gttcggctgc ggcagttcga gccacgacca gaacgcggcc
     4561 ggaaacagga tgacccacgc gccccagacg aggttataga tgcccgcggc ccgcaacgac
     4621 cagacgatcc accgcggcac ctcggcggag gcgcgaggcg gtttgggcac gtcgcaggtt
     4681 tgcaggtcgg cgttcattat cttcggaccg cgaggacacc agcactttaa cagccccggt
     4741 ggtacaggcg caattatcca tcaccgcaga acgaaccggg ggctgctgcg agctacgctt
     4801 gcaaggaaga ctcctgggga ccgctcgacc ccgaggactc gctcgacgcc agcaagcacg
     4861 ccgtcgtcgc cttgtccgtc atcaacttca tctaccagtg ctgcatggag ggcaaggccc
     4921 ttattctgat gtaatcggga ctcccgtccg ctggcgaatg atctccagga caccggtcac
     4981 agactggaaa tccgcggggt cgattcccac gatcaaagag tgctcgccgt acacggccaa
     5041 ctgcggcgaa gtccattgga tgcggctgat cgttgtccag gcgtagaacc gctccttggt
     5101 cagcaggccg tgctcgtgga acccgagcgc cacatcgtag gtccagccgt cgaggcacgc
     5161 gcaccacaag atggcttgaa ccagcacgac ctggacggcg aacccgagca tcggcggaaa
     5221 atcatcctgt gaatcgaaca acggtgcggc gggcaacacc agcaccgaca tcaccacacc
     5281 gtccaggatg accgcagccg tcaaccccca cacggtccac ttccgccgat tgccggtgat
     5341 ggggaatcgc atcacgctca gcagcaactg gcccgcccgc agacggtcgc gccgccgccg
     5401 cagagccacg cgatagccaa tggcaccggc aacacctgcc acggc
//
LOCUS       DASRJB010000554         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2121000, whole genome shotgun sequence.
ACCESSION   DASRJB010000554 DASRJB010000000
VERSION     DASRJB010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121000"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..529)
                     /locus_tag="VFB80_14410"
     CDS             complement(95..529)
                     /locus_tag="VFB80_14410"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN35017.1"
                     /translation="MPRSKLVQTAGLTVAIYWVLMFVGTHVPIWGSPGATLSKLGEGS
                     DKAAHCLAFAGLAFLLCTVASLRWGFRQSFIAAVVGLLLAYSAIDECTQAFVPTRDNS
                     LEDWFADMLGVGLGLSAFSLVCEPLLRRVPRTSPSDDSPAVC"
     gene            612..1577
                     /locus_tag="VFB80_14415"
     CDS             612..1577
                     /locus_tag="VFB80_14415"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZN35018.1"
                     /translation="MSVLVTGGAGFIGSHLVEQLLQTTDRPVVILDNFNDYYDPRLKR
                     AGVATLLGNPRVTLVEGDFCDADLVSRVLLEHEVQAVCHLGASPGVPASLQRPRQNVE
                     NNVVGTVTLLEAARRHPVERFLFASSSTVYGRGAAAPFVEDAPLGIPASPYGASKRAA
                     EILGQTYHQLFGVPFVSLRFFNVYGPRLRPELALAVFTRTILEGTPLPLFGDGSVLRD
                     FTHVSDICRGILAALERPNLAGQAINLGHDRPIRISRLIELIEQAAGAKAKIDHRPPR
                     AEDMPFTHADLTKARRLLGYEPRVGIEEGVREYVAWFRANSLAHP"
     gene            1982..>2520
                     /locus_tag="VFB80_14420"
     CDS             1982..>2520
                     /locus_tag="VFB80_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZN35019.1"
                     /translation="MSPAVAASFGLVAYLALFALVGIVFLFANLLVGRFLRPHDPHPE
                     KLEIYECGEPTIGSSFVQFDLRFYVVALLFIIFDVEVAFFFPWAAVFGKSTNLMDERF
                     KVVERAADGSVALTPQATYLYREMGVSSPTPPQQVPPSLTAEVTVTDDLARADQVIHL
                     GARQLAWVTVVDIAAFFAVL"
BASE COUNT          431 a          814 c          813 g          462 t
ORIGIN      
        1 ggctttgagg cgggggcaac acgtcttagg cggaccaatc agccgcgaat cgtaacagcc
       61 gccgccgcca gcacctagag cgaaaggcgc cttgctagca gaccgcgggg ctgtcgtcgg
      121 acgggctggt ccgcggcacc cgtcgcagca gcggctcgca cacgagcgaa aacgccgaca
      181 agccgaggcc aacgcccagc atgtccgcaa accagtcctc caagctgtta tctcgcgtcg
      241 ggacgaaggc ctgtgtgcat tcgtcgatcg cactgtaggc cagcagcagc cccaccaccg
      301 cggcgatgaa cgactggcga aagccccacc gcaagctggc tacggtacac agcaggaacg
      361 ccagacccgc aaacgccagg cagtgcgcgg ctttatcgga gccctcgccg agcttgctca
      421 gcgtcgctcc cgggctgccc caaatcggca cgtgcgtgcc cacgaacatc agcacccaat
      481 agattgcaac ggtcaggccg gccgtctgga cgagcttcga acggggcatc gggcgagtga
      541 tcctggtggg cggctgccgt cggcaaaccg ctcagtatat catggccccc gcctggcaca
      601 gggaggcctt catgtcggtg ttggttacgg gcggcgcggg attcatcggc agccatctgg
      661 tcgagcagct gctccagacg acggaccggc cggtcgtcat tctcgacaac ttcaacgact
      721 actacgaccc gcggctaaag cgggccggtg tcgcgacgct gctcggtaat ccgcgggtga
      781 cgctggtcga gggcgacttt tgcgacgcgg acctggtcag ccgcgtgctg ctggagcacg
      841 aagtgcaggc cgtctgccat ctcggcgctt cgccgggcgt gcccgccagc ttgcagcggc
      901 cgcggcagaa cgtcgagaac aacgtggtgg gcaccgtgac gctgctcgaa gcagcgcggc
      961 ggcacccggt cgagcggttt ctgttcgcgt cgtcctcgac ggtgtacggc cgcggggcag
     1021 ccgcgccgtt tgtcgaagac gctccgctgg gaataccggc cagcccctac ggcgcgagca
     1081 agcgcgcggc cgaaatcctg ggccagacct atcaccagct gttcggtgtt cctttcgttt
     1141 cgctgcggtt cttcaacgtg tacggcccgc gtttgcggcc ggaattggcc ctggccgtct
     1201 tcacgcggac gatcctggag ggcactccgc tgccgctatt cggcgacggc tcggtgcttc
     1261 gcgacttcac gcacgtcagc gacatctgcc gcggcatcct cgctgccctc gagcggccca
     1321 acctcgctgg ccaggcgatc aacctggggc acgaccggcc gatccgcatc tcgcgcctga
     1381 tcgagctgat cgagcaggcc gccggggcta aagccaaaat cgatcaccgc ccgccgcggg
     1441 ccgaggacat gcccttcacc catgccgacc ttaccaaagc ccgccgccta ctgggctacg
     1501 agccacgggt cggtatcgag gagggggttc gggagtacgt cgcctggttc cgggccaaca
     1561 gcctggccca cccctgaacg gctgcgaacg gacgctcgtg actttgtgaa cattggtatg
     1621 gtatttgcat caaagttccc gatgcgcgcc cgcctgccat tttggcgctc ggcggagcgt
     1681 cagcgcggcg tgtcaggcgc ggcgcaggat ctctctaacg ctcatatata actacttaac
     1741 agcgaatcgg atgattgggc gcaagccatt tagctgcctc acgttgcggc gaaatcggcg
     1801 gaaaattcgc gttgcgaggc ggcactttca ggtggtaacc atgtcgctgc tcggaaccca
     1861 acccgttata ggaaccttgc ggtttccagt agccgccgtc gtcttgtcgg cgagggcgaa
     1921 gggcttacaa tcagcggttt gtttgtgaaa tcgttcacaa ggtcgggaaa ccgggccgtc
     1981 tatgtcgcct gccgtagccg catcgtttgg cttggtcgcg tatctggccc tgttcgcgct
     2041 ggttgggatt gtcttcctgt tcgcgaacct gctcgtcggg cggttcctgc ggccgcacga
     2101 tccgcatccc gagaagctgg aaatctacga gtgcggcgag ccgacgatcg gcagcagctt
     2161 cgtgcagttc gacttgcggt tctacgtggt ggccctgctg ttcatcatct tcgacgtcga
     2221 ggtcgcgttc ttctttccgt gggccgcggt cttcggcaaa tcgacgaacc tgatggacga
     2281 gcggttcaag gtcgtggaac gggcggcgga cggctccgtc gcgcttaccc cgcaggcaac
     2341 ctatctgtac agggaaatgg gcgtcagcag tccaacgccg ccgcaacagg tgccaccgtc
     2401 gctgaccgcg gaagtgaccg ttactgatga tctggcccgg gccgaccagg tgatacatct
     2461 gggagcgcgg caactggcct gggtgacggt ggtcgatatc gcggcgttct tcgccgtgct
//
LOCUS       DASRJB010000555         7069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2694809, whole genome shotgun sequence.
ACCESSION   DASRJB010000555 DASRJB010000000
VERSION     DASRJB010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694809"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2744)
                     /locus_tag="VFB80_14425"
     CDS             complement(<1..2744)
                     /locus_tag="VFB80_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35020.1"
                     /translation="MALSTITLKSLHLLIDRVKRLTDPTAVVSADALDEAYREYDESI
                     RESNDRLHECDELLQGGHRSQALARCEQEPDLLEVTALLDFPERPLWHALLQESGYTA
                     PPAVLWDVASDLNEAYNLEKPLTDLMRMHRLHALAGSPLKKRLGILRKIAERDPGTAI
                     WRDDLRTYERARHLQIEQELSDSEKKRDASLAAALEREVSADDWDEAPPPPLVESAVR
                     IHTQLRQSKAREKIKQIAGQLHDASASRDEAQAQQLRLAWNGQIALAQLPASDPLLEE
                     VEPVFDWLDREEARRQQDKSHKAAIVALEEALDAGAKRIDLERSYNEVARYGPVPEEL
                     DRKVQLRVELLERRGRLRLIAGVIVALALIAVSTGGVFWYIRAQGRAAEFRRRVDALQ
                     QLVAGGKRSEAAALLAEFKAKAPETYSSPEIQTLAGELEFLERKERDRIAARERTLAS
                     IQAAIDEGSWQRLELAMPEIAEAEKLCATDVERREVQDLLARLREGIRTHQDAKNAEF
                     QQLLGELGGKVARVSAADRTTQAALKAEAENLSNMPRVSPELRRHVQPLIERLATIAK
                     GLSAREMEEQALALVSGSIGNLGNYVAALDGYAKSFPQTARAAAFTRVVPEEQPLWTA
                     VEPWNRAVARWSQQDFRKIKPEEAKALLMDLDATAQMHGAFPAAAQLQAARPYLEAVQ
                     RRVNDAGERVHKDLLAVLEDPRVKDLAMLGTIEKKRYYFAETTDKPPQPDSTKEGQWF
                     VIYYKDPQLQETKRTSFLMVLIANPPRGASFDWESPQRRIYKKATAALDQITDATWEK
                     TFLDILATIEAEREMEPTLKLHLTQLFLNMGRQGSHCLALGFKPWLDILANTQADIEA
                     NWIDPDDPLGKKARQTSSDRLARLVPLATCRQAAGIHYVALAPKARGPQYAWVGCLLK
                     "
     gene            complement(2796..5444)
                     /locus_tag="VFB80_14430"
     CDS             complement(2796..5444)
                     /locus_tag="VFB80_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35021.1"
                     /translation="MAKKPPPSPVRRGRPAPPLATAPGGHAARPAAGGGGTVLVRIAI
                     GVTVGVAAALLVTAGTVLGVRMLGRGPQQLAATAPIHPSPDAPTARPSTEPAAEPSSP
                     ASAPAAPEPSLPPAADPAQPASAPAPPAPAAPAAPTPPPATPTPSPAPTPQPIASPSA
                     AAPPAAAAQNSAPAPLSPPAAPAAPDFLLTVKVAGGAGERIEQVRPALTRLDELIAAG
                     GAEQAEFKVKLFDNERTFSSPQEARAGAQAMLLAYFDTISRQYAVTIVTTEVRTLQHG
                     GFWHTKPPQKDDVLVFGLEAVRFLCSFTVEAIRRTGLKNFVLCDEATLNGGRWTPGGA
                     AVQDTIMLEIKNVHPAMGAGLYHELYHVFDQTMLTAQGWAKDAEWLALNPPGQYQGYA
                     AFYGRGAEAQRQAFSQPAPSGFVSVYATASEWEDKAETYSAMMLRSEWLAATIEQDRH
                     IRAKVELIERRLRQLGPEFTPGFWRKSPAPTSVPANPPMAPLAAAPPAAAPAPPTAAP
                     AAAAQPAAAPPGASAIEFVNLPPHKDAGERALLQVGMAAPAQFQLELLGLDEILPKGS
                     VARLEVADPAADVRQWTLLVKAPGALSKEKPLADFRLKAGGLSFQWRLPGSTPPPPPL
                     GYCLLKITGGGDQKICQLIAPADCPPAAVKIGERAPLSIKLPAGACGSATPCQLEVLP
                     QDFPTVQFSGNAALKPGDKTVLKVSGAASGKHPVELELEVEYKLAGPTEAAVSIAAFA
                     STPDTAGKGNNARLREPISLANYERQRKSLLGASKNKESAAKQLAKQIAELERQQTTL
                     TAPGRPQTQLSVQQLAQVRSQLAQAQQERDEADELAQKFAAGHAWYSDMTTLIADLQS
                     KARLGLRVFRPLGGEVVEIARPKAGP"
     gene            complement(5461..>7069)
                     /locus_tag="VFB80_14435"
     CDS             complement(5461..>7069)
                     /locus_tag="VFB80_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35022.1"
                     /translation="KTNPFSRARRRSVWLHYALAGAAGGILAIVAFLLLVVFGPKPEP
                     PIAGLPPAGGGPDTSVPTLPVAATPVVQPDVVPEPVATTPSSDPDASNPPPNPTPEPT
                     PAISSSIPTAPRPMPPPASAKDPFEDILHNKKLKLILPQRPGAGGLTAGSGGASPHLA
                     DVYVQSAAELNLQLLGKEAVFGPEAARVKVEREDKGGASTWRIVKEPAGPLGKKEELG
                     AFKLEGQKLSFQWNRSVQPGAKPDALRHCVLEVNIGGKRQVCQLSEPIRKEGLKLAEL
                     QAGAQTIELTELDSATQSLKLEVQPVDLPAPFVKLPKDEALSPDTEQRILITGGSAGE
                     GPRIELTVKFASDPQRPQRRLIQVSAKATYRTSDVSEEDALKSVGGPLEMKRIAAGVR
                     EYTDKADRVDLNVKRLQTRLDEHKKAAEREDKGIDVAAKSLRSQLAVADGAQKEETKR
                     ELDALMKRKAAFEKKRDELRATLLDGLKQETDERNKFKQMATRFESLGQLMSDTAAKG
                     RLEFRIYFQIDGQVVELYRSQGFPQEL"
BASE COUNT          994 a         2384 c         2368 g         1323 t
ORIGIN      
        1 ttcttcagca ggcagccgac ccaggcatac tgcgggccgc gagccttggg ggcaagcgca
       61 acgtagtgaa ttccggcggc ctgccggcac gtggccagcg gcacgaggcg ggcgagccgg
      121 tcggacgagg tttggcgagc cttcttgccc agcggatcgt cgggatcgat ccagttggct
      181 tcgatatcgg cctgcgtatt ggcgaggatg tcgagccagg gcttgaagcc cagcgccagg
      241 caatggctgc cctggcgccc catgttgagg aagagttgcg tcaggtgcag cttgagcgtc
      301 ggctccatct cacgctcggc ctcgattgtg gccaggatgt cgaggaaggt tttctcccag
      361 gtagcgtcgg tgatttgatc gagggctgcg gttgctttct tatagattcg ccgttgcggg
      421 ctctcccaat cgaacgatgc gccccgcggc ggattggcaa tcaggaccat caagaacgac
      481 gtccgcttgg tctcctggag ctgcgggtcc ttgtaataga tgacaaacca ctggccttcc
      541 ttggtcgaat cgggttgcgg cggcttgtcg gtcgtctcgg cgaagtagta ccgcttcttc
      601 tcaatcgtgc cgagcatcgc caggtccttg acgcgcggat cctccagcac ggcgagcaga
      661 tccttgtgca cgcgctcgcc ggcgtcgttc acgcggcgct gcaccgcctc caggtagggc
      721 cgcgccgcct gcagctgcgc ggcggccggg aacgccccgt gcatctgcgc ggtcgcgtcg
      781 aggtccatca gcagggcctt ggcctcctcg ggcttgatct tgcgaaaatc ctgctgcgac
      841 cagcgggcca ccgcgcggtt ccaaggctcg acggcggtcc atagcggctg ctcctcgggc
      901 acgacccgcg tgaaggccgc ggcccgggca gtctgcggaa aactcttggc atagccgtcc
      961 agcgcggcga cgtagtttcc caggttgccg atcgagccgc tgaccagggc cagggcttgc
     1021 tcttccatct cgcgagccga tagccccttg gcgatggtgg cgagccgctc gatcagcggc
     1081 tgcacgtgcc gccgcaattc gggggaaacg cggggcatat tgctgagatt ctcggcctcg
     1141 gccttgaggg cggcttgcgt ggtgcggtcg gcggcggaaa cccgggcgac cttgccgccg
     1201 agctcgccca gcagttgctg aaactcggcg ttcttggcgt cctggtgcgt acggatccct
     1261 tcgcgcagcc tggcgagcag gtcctgcact tcgcggcgct cgacgtcggt cgcgcagagc
     1321 ttctcggcct cggcgatctc gggcatcgct agctccagcc gctgccagct gccctcgtcg
     1381 atggccgcct ggatggaagc gagcgtccgc tcgcgggcgg caatgcggtc gcgctctttc
     1441 cgctccagga actccagctc gccggccagc gtctgaattt ccggcgagct gtacgtctcg
     1501 ggtgctttgg ccttgaattc ggcgagcagg gctgcggcct cgctgcgctt gccaccggcg
     1561 acgagctgct gcagggcgtc cacgcggcgg cgaaattcgg ccgcgcgacc ctgcgcgcgg
     1621 atgtaccaga agacgccgcc ggtcgagacg gcgatcagcg ccagcgcgac gatcacgccc
     1681 gcgatcagcc gcaaccgccc gcggcgttcc agcagctcga cgcggagctg caccttgcga
     1741 tcgagctctt cggggaccgg gccgtaccgc gccacctcgt tgtacgaccg ctccaggtcg
     1801 atccgcttcg cgccggcgtc gagggcctct tccagggcga cgatcgccgc cttgtggctc
     1861 ttgtcctgct ggcgacgggc ctcctcgcgg tcgagccaat cgaagaccgg ctcgacctct
     1921 tccagcagcg ggtcgctggc cggcagctgg gccagggcga tttgaccgtt ccaggccagg
     1981 cgcagctgct gggcctgggc ctcgtcgcgg ctggccgagg cgtcgtgcag ctggccggcg
     2041 atctgcttga tcttctcgcg ggccttggac tggcgaagct gcgtgtgtat ccgcaccgcc
     2101 gactcgacga gtggcggtgg cggcgcttcg tcccagtcgt cggccgagac ttcccgctcc
     2161 agggcggccg ccagcgacgc atcgcgcttc ttctccgagt cggatagctc ctgctcgatc
     2221 tgcaggtgcc gcgcgcgttc gtacgtccgc aggtcgtcgc gccagatcgc cgtgcccgga
     2281 tcgcgctcgg cgatttttcg caaaatgccc agccgttttt tcagcgggct gccggccagc
     2341 gcgtgcaggc ggtgcatccg catcaggtcg gtcagcggct tctcgaggtt gtaggcctcg
     2401 ttcaaatcgc tggccacgtc ccacagcact gccggcggcg cggtatagcc cgattcctgc
     2461 aacagcgcgt gccacagcgg ccgctcgggg aagtccaaga gtgccgtcac ctcgagcagg
     2521 tccggctcct gctcgcagcg ggccagggct tgcgagcggt ggccgccttg cagcaattcg
     2581 tcgcactcgt gcagccggtc gttgctctcg cggatcgatt cgtcgtattc gcgataggcc
     2641 tcgtcgagcg catcggccga gacgaccgcc gtcggatcgg tcagccgctt gaccctatcg
     2701 atcagcaggt gcagcgactt cagcgtaatc gtggaaagcg ccatacagtc agcccgtcaa
     2761 aagtcccgcc cgtcgcatca gtcccatttg tcccatcaag gcccggcctt cggccgcgca
     2821 atctccacca cctcgccgcc cagcggccgg aagacccgca ggccgagccg ggccttgctc
     2881 tgcagatcgg caatcagcgt cgtcatgtcc gaataccagg cgtgcccggc cgcgaatttc
     2941 tgcgccagct cgtcggcctc gtcgcgctcc tgctgggcct gagccagctg ggagcggacc
     3001 tgggcgagct gctgcacgct gagctgcgtc tgcgggcggc cgggagcggt cagcgtcgtc
     3061 tgctgccgct ccaactccgc gatttgcttg gccagttgct tggccgcgga ctctttgttc
     3121 ttgctcgcac cgagcagcga cttccgctgc cgctcgtagt tggccaggga gatcggctcg
     3181 cgcagacgcg cgttgttgcc cttgcccgcg gtgtcgggcg tgctggcaaa ggcggcaatg
     3241 gaaaccgcgg cctccgtggg gccggcgagc ttgtactcca cctccagctc cagttcgacc
     3301 ggatgcttgc cgctggcagc gccgctgacc ttgagcacgg tcttgtcccc cggcttgagc
     3361 gcggcattgc ccgagaattg cacggtcggg aaatcttgcg gcagcacttc gagctggcag
     3421 ggcgtcgccg aaccgcacgc tcctgcgggc agcttgatcg acagcggcgc tcgctcgccg
     3481 atcttcaccg ccgccggcgg gcagtcggcc ggcgctatca gctggcagat tttctggtcg
     3541 ccgccgccgg tgattttcag caggcagtag cccagcggcg gaggcggcgg agtggagccg
     3601 ggtaagcgcc actgaaacga tagcccgccc gccttcagcc gaaaatcggc cagcggcttt
     3661 tccttgctca acgcccccgg cgctttgacc agcaaggtcc attgccgtac atcggccgcc
     3721 ggatcggcga cttccagccg tgccacagag cctttgggca gaatctcgtc caggcccagc
     3781 agctccaact ggaattgcgc cggggccgcc atgccgactt gcagcagcgc tcgttcgcca
     3841 gcgtccttgt gcggcggcag attgacgaat tcaatcgcgc tggcgccagg cggcgcggct
     3901 gcgggctggg cggcagcggc cggcgcagcg gttggtggcg ccggcgcagc ggctggtggc
     3961 gccgccgcca gcggcgccat tggcggattc gcggggacac tcgtcggcgc tggcgacttt
     4021 cgccagaatc ccggcgtgaa ttccggtccc agctgccgca gtctccgctc gatcagctcg
     4081 accttggcgc ggatatggcg gtcctgctcg atagttgctg ccagccactc gctgcggagc
     4141 atcatcgccg agtaggtctc ggccttatct tcccattcgc tcgccgtggc atagacgctg
     4201 acgaagccgc tgggtgcggg ctgtgaaaag gcctggcgct gcgcctctgc gccacggccg
     4261 tagaaggctg catatccttg gtattggccc ggcggattca aggcgagcca ctcggcatcc
     4321 tttgcccacc cttgcgcggt aagcatcgtc tgatcgaaca cgtggtacag ctcgtggtac
     4381 agacccgcgc ccatggcggg gtggacgttc ttgatttcca gcatgatggt gtcttgaacc
     4441 gccgcgccgc cgggagtcca gcggccgccg ttcagtgttg cttcatcgca gagcacgaaa
     4501 ttcttgagtc ccgtccgccg aatcgcctcc acggtaaagc tgcacagaaa gcggacagcc
     4561 tccagcccaa agaccaggac gtcgtccttc tgcggaggtt tggtatgcca aaatccaccg
     4621 tgctgcaacg tccgcacctc ggtggtgacg atggtcacgg catactggcg gctgatcgtg
     4681 tcgaagtacg ccagcagcat cgcttgggca cccgcccgcg cctcttgcgg gctggagaac
     4741 gtccgttcgt tgtcgaacag cttcaccttg aattcggctt gctcggcccc gccggccgca
     4801 atcaattcat ccaggcgagt gagcgccggc cggacctgct cgatccgctc gccggctccg
     4861 ccagctacct tcacggtcag cagaaagtct ggagcagcgg gagctgccgg cgggctcagc
     4921 ggcgctgggg cactattctg agccgctgct gccggcggag cagcagcgga gggcgaagcg
     4981 atcggttgcg gcgtcggcgc tggggacgga gttggtgttg ctggcggagg tgtcggcgcg
     5041 gccggggccg cgggcgctgg tggagccgga gcgctagcgg gttgggcagg atcagcagca
     5101 ggcggcagcg atggttctgg cgcggcgggt gcagaggccg gagaacttgg ctccgctgct
     5161 ggctcggtgg atggtctggc ggttggcgca tcaggcgacg gatgaatggg ggccgtagct
     5221 gcgagctgct gcgggccgcg gcccagcatc cggactccca gtaccgttcc ggccgtgacc
     5281 agcagcgcag ccgcgacccc gacggtgacg ccgatcgcga ttctcaccag gaccgtccca
     5341 ccgccgccgg ctgcggggcg cgccgcgtgc cccccgggcg cagtcgccag cggcggcgcg
     5401 gggcggccgc ggcggaccgg cgaaggcggc ggctttttgg ccatgcggac ggtcccttgc
     5461 ctacagctcc tgcgggaagc cttggctgcg atacagctcg acgacctgtc cgtcgatttg
     5521 aaagtaaatc cggaattcca agcggccctt ggcggcggta tcgctcatca actggccgag
     5581 cgactcgaac cgcgtcgcca tttgtttgaa cttgttccgt tcgtcggtct cctgcttgag
     5641 tccatcgagc agcgttgccc gcagctcgtc gcgctttttt tcgaaggccg ccttgcgctt
     5701 catcagggcg tccaactcgc gcttcgtctc ctctttctgt gctccgtcgg cgactgccag
     5761 ctggctgcgg aggctcttgg ccgcgacgtc gatccctttg tcttcgcgct cagccgcctt
     5821 tttgtgctcg tccagcctgg tctgcagacg cttgacgttc aggtccacgc ggtccgcctt
     5881 gtccgtgtac tcgcggacgc cggctgcaat ccgcttcatt tccagcggtc cgccgaccga
     5941 cttcagcgcg tcctcctcgg aaacatcgct cgtccggtac gtggctttgg ctgagacctg
     6001 gatcaggcgg cgctgcggcc gctgcgggtc gctggcgaac ttgaccgtca gctcgatccg
     6061 cggaccctcg cccgcgctgc cccctgtgat cagaatccgc tgctcggtgt cggggctgag
     6121 cgcctcgtcc ttgggcagtt ttacgaacgg cgccggcaga tcgaccggct gcacttccag
     6181 cttgagcgac tgcgtggcgg agtcgagttc cgtcagctcg atcgtctgag cccccgcctg
     6241 cagctcggcc agcttcaacc cttctttgcg gattggctcg gacagttggc aaacttgccg
     6301 cttgcctccg atgttgactt ccagcacgca atgccgcagg gcatcgggct tggcgcccgg
     6361 ctgaacggac cggttccact gaaacgacag cttctggcct tccagcttga aggcaccgag
     6421 ctcttccttc ttgccgagcg ggcctgctgg ctccttcacg atcctccagg tgctcgctcc
     6481 gcccttgtct tcgcgctcca ccttgacacg tgctgcttcc ggcccgaaca ccgcttcctt
     6541 gccgagcagc tgcaaattca actcagccgc cgattgcacg tagacatcgg ccaggtgcgg
     6601 cgacgcgccg ccgctgcccg ccgtcagacc gccagcgccg ggccgctgcg gcaggatcag
     6661 cttcagtttc ttgttatgca ggatgtcttc gaatgggtct ttggcggaag cgggcggcgg
     6721 catcggcctc ggggccgtgg gaatcgacga cgaaatggcc ggagtcggtt cgggcgtcgg
     6781 gttcggcggc ggattgcttg cgtccggatc gctgctcgga gtcgtggcga ctggctcggg
     6841 aacaacgtcc ggctgaacca ccggcgtcgc agcaacgggc agcgtcggca cgctcgtgtc
     6901 cggtccgccc ccagccggcg gcagtccggc gatcggcggc tcgggcttgg gaccgaatac
     6961 gaccagcagc agaaacgcga cgatcgccag tatgccgcca gccgctccgg ccagcgcgta
     7021 gtgcagccag accgagcggc gccgcgcccg gctgaaggga ttggtcttc
//
LOCUS       DASRJB010000556         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1282840, whole genome shotgun sequence.
ACCESSION   DASRJB010000556 DASRJB010000000
VERSION     DASRJB010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282840"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1019
                     /locus_tag="VFB80_14440"
     CDS             126..1019
                     /locus_tag="VFB80_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN35023.1"
                     /translation="MISSLPAAEPRTVKVAAIQCSSDLGAVDANRQKLAALVRQAAAK
                     GAKIVVLPETAITGYVSQDLRQNWHLPGRPIEPAFRGRDPLPFAETVPGVSSEQFCAL
                     AKELKIYLTIPLLERVGDLPPTVSLGAGEPPAVFTGKLPKLFNTVCLASPEGKLVAHY
                     RKLNPWPYPEKSWATPGDRGLATFDTEYGRVGLAICFDIHTILEQYRAEKLWALLYPI
                     AWVDDEHPAEWYFHRLPARVGEFRHYLIGANWSVDQPQRWRGYGFSTIIDPAGKVVAS
                     AKSLHGSEIVLAEIEAAQPQR"
     gene            1030..1503
                     /locus_tag="VFB80_14445"
     CDS             1030..1503
                     /locus_tag="VFB80_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35024.1"
                     /translation="MPGEFFPDTNPQAAASPPPAAGSRWWIWLLAIGGFVLISPFCCC
                     GGLIVAASTYKDFALSNGQHLGGPPMNVRFDYEIRNDRLMFDTFYIVCKSADGTSRER
                     NLAGSFAKRGTLHFNAFDLGPASTVRGPVQVWIEKADMRGGRSRASNVLTIYTKT"
     gene            1508..2167
                     /locus_tag="VFB80_14450"
     CDS             1508..2167
                     /locus_tag="VFB80_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35025.1"
                     /translation="MRSFQISLRKAEKHLAAADAKMARLIDTHGKCALAPYWDRSPYE
                     SLVRAVAYQQLHATAAQAILGRLVARFTGQPFPAPADIVALSAEELRPLGFSLAKATT
                     IRGIAEGALSGVVPARAAAEALSDEELIDRLTSLRGIGRWTVEMLLIFTLGRLDVMPI
                     DDFGVRAGLAAVYRIRDKVTKHHFPRRTDRWAPYRSIGAWYLWREADRLKAAAKKKAA
                     V"
     gene            complement(2133..2906)
                     /locus_tag="VFB80_14455"
     CDS             complement(2133..2906)
                     /locus_tag="VFB80_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35026.1"
                     /translation="MALIELRDVWKVYDLGEVKVEALKEATLDIEQGEYIALIGPSGS
                     GKSTLMNTLGCLDRPTKGSYKLAGEEVATMSRDQRARIRNQRLGFVFQNFNLLARTTA
                     LENVELPLLYSRGVSAHERKRRAIDKLNLVGLADRLDHHPTQLSGGQQQRVAIARALV
                     NEPAILMADEPTGNLDSRTSREVIGLFRQLNEQQKITVILVTHDQDVARHARRTIVLR
                     DGRVVADTADLSQALAALHASHNGDGAAATPLPSSSPQP"
     gene            3128..4087
                     /locus_tag="VFB80_14460"
     CDS             3128..4087
                     /locus_tag="VFB80_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35027.1"
                     /translation="MLRRPTKTARRSFRPVVETLEGRDMPSVFLGRFDTDGDGGGEDV
                     FQDSSRVEIREANGATRNYFVGTQGVFTTATNTDGLAGVELVFSTGQGVRIIADRTRT
                     TRDYVTGSTAVFLTAANTDGLAGNELVYDIGPSVRIIHDSTRRVSDTLIGTEWELIAV
                     ADTNGLVGEELVFDVFGSIRIYRDANRTLKTYNVAASWELLTVADTDGKPGKDLVLVV
                     DQFLRILHDASGTITNHFLGGPFTLLDVVDTDGKAGRELVFSQGNTIKIIRDATRTSR
                     IYFLDPPFVYLGAAQLDGVAGFELRFNSNGRIFVLNDRLQTLR"
     gene            4371..>4714
                     /locus_tag="VFB80_14465"
     CDS             4371..>4714
                     /locus_tag="VFB80_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN35028.1"
                     /translation="MFVAASTECFGDLSLPLALERLADLEYTSVELALYEDANQLRPS
                     QVAANVEEAVATCRNTQRLDVVAFDTRITAEKAAHYEQFTAICRLAKAAKVVTLSVPS
                     AELGTPFNEEVE"
BASE COUNT          803 a         1560 c         1518 g          833 t
ORIGIN      
        1 ttgcactctc gttacttgac cgcggtgcac tcgcctccaa tcattcgccg tgggccggcg
       61 gtggaggcat tacgatggcc gcgcgctgca gtatggtggt ggttccgctt gcgggactgc
      121 tgctgatgat ttcatcttta ccggccgccg agcccaggac cgtgaaggtc gcggcgattc
      181 agtgctcgtc ggacctgggc gctgtcgatg ccaaccggca gaagctcgcg gctctcgtgc
      241 gacaagcagc cgcgaagggt gcgaagatcg tcgtcctgcc cgaaacggca atcacgggct
      301 acgtatcgca ggacttgcgt caaaattggc atttgccggg ccgcccgatc gagccggcgt
      361 ttcgcggccg cgatccgctc ccgttcgccg agaccgtgcc gggcgtcagc agcgagcaat
      421 tctgcgccct ggcgaaggag ctgaagatct acctgacgat cccgctgctg gagcgcgtag
      481 gcgatctgcc gccgaccgtt tcgctgggcg cgggcgagcc gcccgcggta ttcaccggca
      541 agctgcccaa gctgttcaac acggtctgcc tggccagccc ggaaggcaag ctcgtggcgc
      601 actaccgcaa gctgaatccc tggccctatc cggaaaaatc gtgggccacg cccggcgacc
      661 gcggcctggc gactttcgat acggagtacg gccgcgtcgg tctggcgatc tgcttcgata
      721 tccacacgat cctggagcaa tatcgagccg agaagctctg ggccctgctc tatccgatcg
      781 cctgggtgga cgacgagcat cccgccgagt ggtacttcca ccggctgccc gctcgcgtcg
      841 gcgaattccg ccactacctg attggcgcga actggagcgt tgatcagccg cagcggtggc
      901 gcggctacgg cttctcgacg atcatcgacc cggcgggcaa ggtcgttgcc tcggccaaga
      961 gcttgcacgg ctcggagatc gtcctggcgg agattgaggc ggcgcagccg cagcgataga
     1021 ctttgccgga tgcccggcga attctttccc gacaccaatc cgcaggccgc ggccagcccg
     1081 cctccggccg ctggctcgcg gtggtggatc tggctgctgg cgatcggcgg gttcgtgttg
     1141 atctcgccct tctgttgctg cggcggcttg attgtcgctg cttcgaccta caaagacttc
     1201 gcgctgagca acgggcagca tctgggcgga ccgccgatga acgtgcggtt cgactacgag
     1261 attcgcaacg accgcctgat gttcgacacg ttctacatcg tttgcaaatc ggccgacggg
     1321 acgtcccgcg agcgcaacct cgccggcagc ttcgccaagc gaggcacgct ccacttcaac
     1381 gccttcgatc tcggtccggc gagcacggtc cgcggaccgg ttcaggtctg gatcgagaag
     1441 gcggacatgc gcggcggacg cagccgggcc tcgaacgttc tcacgattta cacgaagacc
     1501 tagccgcatg cgttcgttcc aaatatcgct ccgcaaagcg gagaagcacc tcgcggccgc
     1561 cgatgcgaag atggcccggc tgatcgacac gcacggcaag tgcgcgctgg cgccctattg
     1621 ggaccgctcg ccctacgaga gcctcgtgcg ggccgtggcc taccagcaac tgcacgccac
     1681 cgcggcccag gcgattctcg gccgcctcgt ggcccggttt acggggcagc cgtttcccgc
     1741 gccggccgac atcgtggcgc tgagcgccga ggaattacgg ccactgggct tttcgctggc
     1801 caaggcaacg acgatccgcg gcatcgccga aggagcgctt tcgggagtcg ttcccgcgcg
     1861 ggccgccgcc gaggccttga gcgacgagga gctgatcgat cggctgacct cgctccgcgg
     1921 gatcggccgc tggaccgtag agatgctgct gatcttcacg ctcggccgtc tggatgtgat
     1981 gccgatcgac gatttcggcg ttcgcgccgg cctggccgcg gtctatcgca tccgcgacaa
     2041 ggtgaccaag caccacttcc cccgccgcac cgatcgctgg gcgccctacc gctcgatcgg
     2101 cgcgtggtac ctgtggcgcg aggccgaccg gctcaaggct gcggcgaaga agaaggcagc
     2161 ggtgtagcgg cggccccatc gccgttgtgc gaggcgtgca gggccgcgag ggcctgcgaa
     2221 aggtccgccg tatcagcgac gacccgtcca tcacggagca cgatcgtgcg gcgggcgtgg
     2281 cgggccacgt cctggtcgtg cgtcaccagg atcaccgtga tcttctgctg ctcgttgagc
     2341 tgccggaaca ggccgatcac ctcgcggctg gtgcggctgt cgaggttgcc cgtcggctcg
     2401 tcggccatca ggatcgccgg ctcgttgacc agggcccggg cgatggccac acgctgctgc
     2461 tggccgccgg agagctgtgt ggggtgatgg tccagccggt cggccaggcc gacaagattc
     2521 agcttgtcga tcgcccgccg cttccgctcg tgggccgaga cgccgcggga atagagcagc
     2581 ggcaattcga cgttttccag ggccgtcgtg cgggccagca gattgaagtt ttgaaagacg
     2641 aaccccagtc gctggttgcg gatgcgggcc cgctggtcgc gggacatcgt ggcgacctcc
     2701 tcgcccgcca gcttgtagct ccccttcgtc ggccggtcga ggcagccgag cgtattcatc
     2761 agcgtcgatt tgccggagcc ggagggtccg atcagggcga tgtactcgcc ctgctcgata
     2821 tcgagcgtcg cctccttgag ggcctcgacc ttgacctcgc ccaagtcgta gaccttccag
     2881 acgtcgcgga gctcgatgag ggccaaggaa atcggtccgg acgaaaaagg gcgggattaa
     2941 gattaggatt aagattaaga acaggacggt ggatcgcggt cctaatctta atcctaatct
     3001 taatcctaat ctgcctgctc tctgctctcc gccgccccca caatcgccgg cataaaagtt
     3061 gccttgcggt cctcctcgaa gcatagtggg cgcgatatca ggcgattccc cgagaggagt
     3121 gctagccgtg ttgcgccgtc cgaccaaaac cgcccgccgc agttttcgtc ccgtcgtcga
     3181 aacgctcgag gggcgcgata tgcccagcgt cttcctcggc cgcttcgaca ccgacggcga
     3241 cggcgggggc gaagacgtgt tccaggacag ctcgcgggtg gagattcgcg aagccaacgg
     3301 cgccacgcgg aactacttcg tgggcacgca gggcgtcttc acgacggcca ccaacaccga
     3361 cggcctggcc ggtgtcgagc tggtcttcag caccggtcag ggagtccgca tcatcgccga
     3421 ccgcacgcgg acgactcgcg actacgtcac cggctcgacc gcggtatttc tgacggcggc
     3481 caacaccgac ggcctggcgg ggaacgagct ggtttacgac atcggcccgt cggtccgcat
     3541 cattcacgac tcgacgcgcc gcgtcagtga cacgctgatc ggcacggagt gggagctgat
     3601 cgcggtggcc gacaccaacg gcctggtcgg cgaggagctg gtgttcgacg tcttcggctc
     3661 gatccgcatc taccgcgacg ccaaccgcac gctcaagact tacaacgtcg cggccagctg
     3721 ggaactgctg accgtagccg acaccgacgg caaaccgggc aaggacctgg tgctggttgt
     3781 cgatcagttt ctgcggatct tgcacgacgc ctcgggcacg atcacgaacc acttcctcgg
     3841 cgggccgttc acgctcctgg acgtcgtcga taccgatggc aaagcgggcc gcgagctggt
     3901 gttcagccag ggcaacacga tcaaaatcat ccgcgacgcc acccgcacga gccggatcta
     3961 tttcctcgac ccgccgttcg tctatctcgg cgcggcccag ctcgacggcg tcgccggttt
     4021 cgagctgcgg ttcaattcca acggccggat tttcgtgctc aacgaccgcc tgcaaacact
     4081 gcgctaagta agcgagagac ctgacagcca tcgaggctca cccggcgatt cagcgccgtt
     4141 cgatttaacg caaagcagca aaggagcaaa gacgcgatgc gagcactctc aggtctgctc
     4201 tgcgcctttg gccccttcca tccttgcgtt agatgagagg tcgttagatt tcccgcccgc
     4261 tcccgattct cgaccccgac ctgccgttgc ccaaagcggc gcggccgcgt taaactacgt
     4321 cgcgcccgcc tggggtggca cggacggact gccaacgaag gagtgttctg gtgtttgtcg
     4381 cggcttcgac ggaatgtttt ggcgacttgt cgctcccgct ggcgctagag cggctggcgg
     4441 acctggaata tacgagcgtc gagctggcgc tctacgaaga cgccaatcag ctgcggccct
     4501 cgcaagtcgc ggccaacgtc gaagaagccg tggcgacctg ccgcaatacg cagcggctcg
     4561 acgtggtcgc cttcgacacg cggatcaccg ccgagaaagc cgcccactac gagcagttca
     4621 ccgcgatctg ccgcctggcg aaggccgcga aggtcgtcac cctcagcgtc ccctcggccg
     4681 agctgggcac gccgttcaac gaagaggtcg agca
//
LOCUS       DASRJB010000557        14886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1846186, whole genome shotgun sequence.
ACCESSION   DASRJB010000557 DASRJB010000000
VERSION     DASRJB010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846186"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /gene="asnB"
                     /locus_tag="VFB80_14470"
     CDS             complement(<1..1628)
                     /gene="asnB"
                     /locus_tag="VFB80_14470"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN35029.1"
                     /translation="MCGIVGLYERNSGSSADALREAAAKMAGCLVHRGPSEGGVWADP
                     AAGIALGHRRLSILDLSPAGSQPMTSACGRFTIVYNGEVYNHQELTAELRAAGQAFRG
                     HSDTEVLVEAIARWGVQSAVERANGMFAFAVWDAQERTLTLVRDRLGIKPLYYAWVGE
                     TFVFASELKSLVVHSQFRPEIDRDALAQFLQHSYIPAPATIYQGVWKLPPGCSLTIGP
                     DSPKSAKPVRYWSVEGAVERGRQKPFRGTAAAAVSELESLLRDSVARQRLADVPVGAF
                     LSGGIDSSVVVGLMQAQGALSVRAFTIGFDEPDYDESPFAEAIARHWQIDHVIERVTA
                     QEARAVIPQLPAIYDEPFADVSQIPTFLVSQLARRHVTVSLSGDGGDELFGGYDRYAR
                     IDAIWKRIAWLPAPLRRLASRLYQNVMHERLLGRSGQSFRARALGVTDRRELYAELHR
                     HWERPAEVVIGARGSSSVIDGIRSVPATFLEQMMALDTATYLPDCILTKLDRASMAVS
                     LEARVPLLDHRVVEFAWTLPLEFKVRAGTSKWPLR"
     gene            1748..3232
                     /locus_tag="VFB80_14475"
     CDS             1748..3232
                     /locus_tag="VFB80_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZN35030.1"
                     /translation="MGLVTGLTLVQLLVQFVLQRILAGQFGSTAEMDAYVAVLALPVV
                     ISAMLAGSLGYVLVPVFAERRSDAGEQAAAMVTAQIGLYLLAISLVVAAAVAVAAQPI
                     AGLLCPGFTPGQLSLAARLLRILAGLIVANSLVSFLNALHQCLRQFALPAAGGVIGTL
                     VTVGYVALFIERQGIDAAAYGVLIGGLTTAAILVPPWLLQLRERQAWTLVPGAAVRRC
                     LTLLVPLVLGSIYWRLDPLLDRYLGSFLSAGSLSHLGYAGRLTGALMLVGTSGLSAVT
                     FPALAGHAAEGRRAELNAEIAHAWRLLIVLLTPVVLGLIGFSEPVTRLLYEGGAFSAR
                     DTQQVARLIVLSVGMIVGAGCGDLLSRVMYALHDTRTPVVVGFAGFMLGAALKFGLLR
                     SWGAAGLVAATSTFYLFHAAVLTAVLLHRLGGDMLAGAAACLARSGVSAALACVAAWA
                     VIQVPLTWAVLPAAAAGAVTYGLALWLMGDEFARRFLQMIAGSR"
     gene            3235..4755
                     /locus_tag="VFB80_14480"
     CDS             3235..4755
                     /locus_tag="VFB80_14480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN35031.1"
                     /translation="MPKPAVKSLFSFRWGRGRVYLLPLRGLAYGAISLAVVAALLLFI
                     DRVVLGTLERGGWTAGEMWRRNNQLVEENHRRKTLGDVDGLRQWRGEPLPAAASTGER
                     PTILVLGDSFVFGPPYPTLNQLWWRQLAIELERRGYRQVQVLAAGRSGWSTHDQLQRA
                     RTLIPEIKPDLVLWGYVTNDPDEKLVPQIADRNDRGPLPHRVRMQLQALLPNLMFKFE
                     SLRADKLATQYAGPKYGYRYFDWEQKLLEGDNFARYRETVAALGKLLGNERTPGVMIT
                     LPGWPCREHYEPRYAPVLPLWRSAGVAVCETLEPFLSRYGNAPQNGPEAIRWGINPAD
                     SHPGPRATHFHAVMAADYLEQHHAELLGPKDAARPHELAINDWLPFDLNVRPHGAGEF
                     LLDYPATTQFMPTLPLDVPTALVALRYPLSVNEIRISGAGLRGVRVWISTLHPAENYD
                     ECQWRELPRASGGEFSFRLPADLAGCALTTVRFSADALGASRELTLTLVPAAGEQP"
     gene            4755..6584
                     /locus_tag="VFB80_14485"
     CDS             4755..6584
                     /locus_tag="VFB80_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HZN35032.1"
                     /translation="MVRILGISAFYHDSAAALLEDGQIVGAAQEERFTRIKGDASFPH
                     RAIGWCLDQAGLGAGDVDHVVFYEQPVVKFERLLVSYLATAPRGWASFLRSMPAWLTS
                     KLWLEREIARELGIRKRVWFTTHHMSHAASAFFPSPYEEAAILTIDGVGEWSTATWGV
                     GHGNRIELAEEMRFPNSLGLLYSAFTYYCGFKINSGEYKLMGLAPYGQPKYAAEILDK
                     LVSLHDDGSLLLNQAYFNYAGGLTMTNERFHRLLGGPPRPPESLLTQREMDLAASVQE
                     VVNKAILRMGRYVHERTGLPHLTLAGGVALNCVATGKLSLEGPFESLWTQPAAGDAGG
                     ALGAALWFWHHRLAAPRVAPQPDGMHGAFLGPEIPPESADDDALLRRLGAVWDELQGD
                     ELAERIAAEIEAGKVVAVARGKMEFGPRALGARSILGDARSPSMQSHMNLKIKFREGF
                     RPFAPMVLAEDAERYFGARQESPYMLLVYPVSEHLRRPVQADGKFGIELLQQPRSEIP
                     AVTHVDFSARVQTVDRQRNPLLESVLRRFKGRTGCSVIVNTSFNVRGEPIVNTAEDAY
                     RCFMATDIDCLVVGNRFLARDRQQNAPLNQDERRRWLQRFDLD"
     gene            6544..7230
                     /locus_tag="VFB80_14490"
     CDS             6544..7230
                     /locus_tag="VFB80_14490"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZN35033.1"
                     /translation="MSAAAGSSVSTSTEGRPRAPDAAAPRPRARGAYAADLARRREAA
                     AIKRQIGYGLVVGWILALVGGFFFYTVTSPVDWLWGSLMVVGWLHLVLAVVLPQALYW
                     PERAWSGLAQWQGKLVMAVLLTVVYFGLIWPARWLRRSAEPGFFAWDNERPGSGSAWQ
                     PLDSAEADPKLVHERQRSLPLLLAGVIAFFFRRGHFILLPILILLLVLGLVLFFVQSS
                     ALAPFIYTLF"
     gene            complement(7259..8944)
                     /locus_tag="VFB80_14495"
     CDS             complement(7259..8944)
                     /locus_tag="VFB80_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35034.1"
                     /translation="MTTVDLPAAAPKEAEATAPPAAPADRWAATWLQFETLLDRAGDR
                     LNPILVKEARQSMKSRQFVVTFSLLLVFGWLWTVLFISFGVPAIFYAPVGPGVLMGYY
                     VVLSIPLLIVVPYAAFRSLAAEREDGTFELLSITALSSRQIVLGKLGSAILQMMVYYS
                     ALAPCIAFTYLLRGIDIITIGLFLAYTFLASLLLSIFGLMMATVTRARHWQVLLSVVF
                     VMALLVFTFVWDMSFVSLLASRAAMPLDQVEFWIANLCMLSFYAPLATLLLFISGGQI
                     TFASENRSTPIRMVLIVPQFLLVGWMIFFWVAYRVEEGFYVWTTVAAIYWAIAGAFLS
                     GEMAHLSPRAKRQLPQSFLGRTLFTWFNPGSGTGYVFAMLNLLSVMSVAWFAVLYGSI
                     LGQEVPSFTEWFCYTISLTGYVLAYIGIVRLAIVALRRVTPVGLLATFLCQVIVASGG
                     VLFPLLLQTIISWGDYTTFGYTMLQLPNYFWTLYEIAERSSFGGWELAIIIGSVGGAI
                     FLLNLLVAAGEVEHVRQQAPQRVLEDERQLHPQPEQRKKRNPWDEEPVSSEAT"
     gene            complement(8952..9896)
                     /locus_tag="VFB80_14500"
     CDS             complement(8952..9896)
                     /locus_tag="VFB80_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35035.1"
                     /translation="MVATHSMIELRRLHRFFGSTRAVSDVTFEVGRGQVFGYIGPNGA
                     GKTTSMRILATLDLPTAGDAFVDGFSCVNDPDRVRRRLGFMPDYFGTYADVNCYEYLD
                     FFARSYGLVGRERHQAVRHTMSFTRLDELAEKPIRGLSKGMRQRLCLGRAMIHDPSVL
                     ILDEPANGLDPRARIELREMIRLLAADGKTVLVSSHILTELAEMCDEVGIIERGQLLA
                     VGTVAEIQKQQQPHREVKVRVLGGAAALALWLGQRSDVTHVKADGELVRFTHEGEQDT
                     EVALLREIVLANFAVAEFGSTNKSLEDVFMAVTRGAVQ"
     gene            complement(9900..12272)
                     /locus_tag="VFB80_14505"
     CDS             complement(9900..12272)
                     /locus_tag="VFB80_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35036.1"
                     /translation="MVRRTRFPGLFAALAAALICGLLATAARAGSGPLFSLPQVGVKN
                     KSGLIISLDGRGVEGNGYRPVRVEVTPWPPKPLTADRQIRIVIKPNSYNAASTPQVSQ
                     VIDLPEGSMQVEAILSVPQSALWYSMAIETYEGGEKLEDLSHPYMGWANVNYWDWTED
                     RPTMLFIDSQVLARAARDPAVAAFKSTSADPTPTNNLPDVRTMAYLFPDPSRGMPVGS
                     PGTSPGMLLATTPKPTDVMLLSQIADMGRVEMLPPAELPERWIDLSQYDVITIRFGDL
                     KDLAAKQPQTFAALRQWLSTGPLLNVYDVGDKFERLADLEQLLKLPPLPAGEEQPAEH
                     RGWTAPDTNMKKHELRSAWEAQDYELTQQYPGGGYSKTIRNKRKPTAAIAPKSKDAKS
                     KDDSPLLEKPPFLFRRAGVGCVAAISAAKPFPGQDTDWILLFNSVPDNHWKWFRRTGF
                     SQHRTNDDYWTFLIPGVGEAPVVSFLLLVSLFAIVIGPVNYVLLGRSRRLYLLLLTVP
                     AGALLVTLCLFTYALLTDGLGVRLRSRSFTALDQRTGEAAAWSRQSYYAAIAPSQGLT
                     YPNDTTVFPLEYEPGYRGSNPNGTTGKLVWDEDQHLTSGYLTSRTATQFMIQRATKTP
                     ALLVVKEGAAAGQPPEIENRLESEIRYLLLRDSRGDYFAGKMVGQDAKAKLAKIDPAA
                     AEKELRKLADAVEPDLPRGYDPNIHNNNALSFLNGNYGGAWNNVDASAAKPLMAASLL
                     EKNIVECILPSKRPLEPGSYTAVVDASPAVPIGVPRVREEASLHVIRGKY"
     gene            complement(12291..12620)
                     /locus_tag="VFB80_14510"
     CDS             complement(12291..12620)
                     /locus_tag="VFB80_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35037.1"
                     /translation="MIIPQFSLRWLLGLTALCAGISLILSFAMRGDGWAVGSAAALGS
                     VAAVAILHVAAFLGAWLVSQMSIGLFGRRAEGQSPFANKTVAEAPFEGAGLVESPMTE
                     GPPPITG"
     gene            12943..14820
                     /locus_tag="VFB80_14515"
     CDS             12943..14820
                     /locus_tag="VFB80_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35038.1"
                     /translation="MSDLPAPPESPQNSNWALIRRMIGLGWQYRWGCLRLLCLQGLLL
                     LAALGTLRLTGLGIDLIRFYAHASLDKPPIPFGLTLPFSSPLAQVALLAALVLLLEAI
                     RGSLNYVYALSAGYLVHTRIVPQLRSLVYDKLQRLSFRFFDANATGSIINRVTSDVQS
                     VRSFIDGVLIQFVLLLVSLVCYLGYMLSLNVGLTLACLATTPLMWLVTVIFSRMVRPL
                     YDRNRDLVDRVILRLAESIQGVQVIKGFGREREEIARFAADNRAVRDQQHGIFWRVSI
                     FGPTIGYMTQINLVILLAYGGWLVTQDRLALGSGLVVFAGLLQQFSSQVANLTNIANS
                     VQQSLSGARRVFEILDTPIAIHSPPNAVRLVARDSVAGTRHGVAGYGGRIEFDHVWFE
                     YTPGNPVLKDVTFIVEPGQKVAILGATGAGKTTLLALLCRFYDPTRGRILLDGHDLRD
                     LDLDDLRRHIGLVFQETYLFSNTVAANIAFGHPEATLGQVQASAKIAAAHDFIAALTH
                     GYDTVLGEGGLDLSGGQRQRLAIARAVVLDPTLLLLDDPAAAIDPHTEHEILSAMERA
                     MQDRTTLVIAHRLSTLRQCDRVIVLEHGRIAQRGTHAELMEADGHYRHAAESQLAGAR
                     A"
BASE COUNT         2431 a         4873 c         4947 g         2635 t
ORIGIN      
        1 cgcagcggcc acttgctcgt gcccgcgcgg actttgaact ccagcggcag agtccaggcg
       61 aattccacca cgcgatggtc cagcagcggt acgcgggcct ccaggctgac cgccatgctc
      121 gcccggtcga gcttcgtcag gatgcagtcc ggcagatacg tcgccgtatc cagggccatc
      181 atctgctcca gaaacgtagc aggcacactc cgtatgccgt caattaccga agatgaacca
      241 cgggcgccga tcaccacctc cgccggcctc tcccagtgcc ggtgcagctc ggcatacagc
      301 tcgcgccggt ccgtcacgcc cagtgcgcgg gcccgaaagc tctgcccgct tcggcccagc
      361 agccgctcgt gcatgacgtt ttggtaaagc cgtgaagcga gcctcctgag cggagcgggc
      421 agccacgcga tccgcttcca gatggcgtcg attcgtgcgt agcggtcgta gccgccgaac
      481 agctcgtcgc cgccgtcgcc cgacaggctc acggtcacgt gccggcgggc gagctgcgat
      541 acgaggaacg tcggaatctg cgacacgtcg gcaaaaggct cgtcgtagat cgccggcagc
      601 tgcgggatga ccgcgcgggc ctcttgcgcc gtgacgcgct cgatcacgtg gtcgatctgc
      661 cagtggcggg cgatcgcctc ggcaaacggc gactcgtcgt aatcgggctc gtcaaagccg
      721 atcgtaaagg cccgcacgct gagcgcccct tgggcctgca tcagcccgac gacgaccgag
      781 gaatcgatgc cgcccgagag gaaggccccg accggcacat cggccagccg ctgccgggcg
      841 accgagtcgc ggagcaggct ttccagctcg ctgacggcag cggcggcggt gccgcgaaat
      901 ggcttctgcc ggccgcgctc gaccgcgccc tcgaccgacc aatagcggac cggctttgcc
      961 gactttggcg agtcgggacc tattgtcagc gagcatcccg gcggcagctt ccacacgccc
     1021 tgatagatcg tcgccggcgc ggggatgtaa ctgtgttgca agaattgcgc cagcgcgtcg
     1081 cggtcgatct cgggccgaaa ctgggaatga accaccaggg acttgagctc cgacgcgaat
     1141 acaaacgtct cgccgaccca ggcatagtag agcggcttga ttcccagccg gtcgcggacg
     1201 agcgtcaacg tccgctcttg ggcgtcccaa acggcgaacg cgaacatccc gttggcccgc
     1261 tcgaccgccg actgcacgcc ccagcgagcg atcgcttcca cgaggacttc ggtgtcggag
     1321 tgcccgcgaa acgcctggcc cgcggctctc agctcggccg tcagctcctg atggttatag
     1381 acctcgccgt tgtagacgat cgtgaatcgg ccgcaggccg aagtcatggg ctggctgccg
     1441 gccggcgaga ggtcgaggat cgacagccgg cggtggccca aggcgattcc cgccgctgga
     1501 tcggcccaga cgccgccttc gctcggcccg cgatggacca ggcagcccgc cattttcgcg
     1561 gcagcctcgc gcagggcgtc tgcggaagag ccggaattcc gctcatacag tccgacaatc
     1621 ccgcacattc cttttgtctt tccggtcggt tgtggaacac tagattacag gttttttggc
     1681 ctcgccccgc cacccagagc cggcaattcg cttgaactcc ccccagcccc aaagttcgct
     1741 gcggtctgtg gggctcgtca ccggcctgac gctggtgcag ttgctggtgc agtttgtcct
     1801 gcagcggatt ctggcgggcc agttcggctc cacggccgaa atggatgcct acgtggcggt
     1861 gctggccttg ccggtcgtca tctcggccat gctggcgggg tcattgggct atgtgctcgt
     1921 gccggtcttt gccgagcgcc gcagtgacgc cggcgagcag gctgccgcga tggtgactgc
     1981 ccagatcggc ctctacctgc tggcgatctc gctggtcgtg gcagcggcgg tcgccgtggc
     2041 ggcgcagccc atcgccggcc tgctctgccc gggatttacg ccagggcaat tgtcgctcgc
     2101 cgccaggctg ctgcgaatcc tcgcgggact gatcgtcgcc aacagcctgg tcagctttct
     2161 caacgctctg caccagtgcc tgcggcagtt cgcgctgccc gcagccggcg gcgtcattgg
     2221 cacgctggtc accgtcggct atgtggcgct gttcatcgag cggcagggga tcgacgccgc
     2281 ggcttacggc gttttgatcg gcgggctgac cacggcggcc attctcgtgc cgccgtggct
     2341 tttgcaactg cgcgagcggc aagcctggac gctcgtcccc ggcgccgcgg tgcggcggtg
     2401 cctgacgttg ctcgtgccgc ttgtgctggg ttcgatttac tggcggctcg atccgctgct
     2461 ggaccgttat ctgggttcgt ttctctcggc gggaagcctg tcgcatcttg gctacgccgg
     2521 gcggttgacc ggcgcactca tgctcgtggg caccagtggc ctttccgccg tcacgttccc
     2581 cgcgttggcc ggccacgcgg ccgaaggccg gcgggcggag ttgaacgccg aaatcgccca
     2641 cgcctggcga ctcttgatcg tgctgctcac accggtggtg ctgggcctga tcggcttcag
     2701 cgagcctgtt acgcggctgc tctacgaggg gggcgcgttc tcggctcgcg acacgcaaca
     2761 ggtcgcccgc ttgatcgtgc tgtccgtcgg aatgatcgtc ggggccggct gcggcgacct
     2821 gctctcgcgg gtgatgtacg ccctgcacga cacccggacg cccgtcgtgg ttggatttgc
     2881 cggattcatg ctcggggcgg cgctcaagtt cgggctgctg aggtcgtggg gagcggcggg
     2941 cctggtggcc gcgacgtcca ccttctatct gttccacgca gccgtgctga cggccgtttt
     3001 gctccatcgt ctgggagggg atatgctggc aggagcggcg gcttgcctcg cacggagcgg
     3061 ggtgagcgcg gcgctagcgt gcgtggcggc ctgggccgtc attcaggttc cgttgacctg
     3121 ggcggtgctg ccggcagccg cggcgggcgc agtcacgtat ggtctggcac tttggctaat
     3181 gggcgacgaa tttgcccgcc gctttttgca aatgatcgcc ggatcgcgct aacgatgccc
     3241 aagccagcgg tgaaatcgct cttttccttt cgctggggcc gcgggagggt ctatttgctg
     3301 ccgctgcgcg gcctggccta tggggcgatt tcgctggcgg ttgtggcagc cctgctgctg
     3361 tttatcgacc gcgtggtctt ggggacgctg gagcgcggcg gttggacggc gggcgagatg
     3421 tggcggcgca acaaccagct tgtggaggag aatcaccgcc gcaagacgct gggcgatgtg
     3481 gacggcttgc ggcaatggcg gggcgagccg ctaccggccg ccgcttccac cggcgagcgt
     3541 cccaccattc tcgtgctcgg cgactcgttc gtgttcggcc cgccgtatcc gacgctcaat
     3601 cagctgtggt ggcggcaact ggcgatcgaa ttggagcggc gcgggtatcg tcaggtgcaa
     3661 gtgctcgccg caggccggtc gggctggtcc acgcacgacc agctgcagcg agcacggacg
     3721 ctgatccccg aaatcaagcc agatctcgtc ctctggggct acgtcaccaa cgaccccgac
     3781 gagaagctgg tgccgcagat cgccgaccgc aatgaccgcg gcccgctgcc gcaccgcgtg
     3841 cggatgcagc tccaggcgct gttgccgaac ctgatgttca agttcgagtc gctgcgggcc
     3901 gacaagctgg caactcagta tgccggcccg aaatacggct atcggtactt cgactgggaa
     3961 cagaagctgc tggaggggga caactttgcg cgctatcgcg aaacggtcgc cgctcttggc
     4021 aagttgctcg gcaacgagcg tacgccgggc gtgatgatca cattgcctgg ctggccctgc
     4081 cgcgagcatt atgagccacg ctacgcgccg gttctcccgc tgtggcgttc cgcaggggta
     4141 gcggtctgtg agacgctcga gcctttcctc agccgctatg gcaacgcccc acagaacggg
     4201 ccggaggcaa tccgctgggg gatcaacccg gccgacagcc atccggggcc gcgggcaaca
     4261 cactttcacg ccgtaatggc ggccgactac ctggagcagc atcacgcgga attgctgggt
     4321 cccaaagatg ccgcgcggcc gcacgagctg gcgatcaacg attggctgcc gttcgatttg
     4381 aacgttcgtc cgcacggggc cggggaattc ctgctcgatt atccggccac gactcagttc
     4441 atgcccacgt tgccgctgga tgtgcccacg gcgctggtgg cgctgcgcta cccgctgagc
     4501 gtgaatgaga tccggatttc gggcgcaggt ctgcgaggcg tacgcgtgtg gatttcgacg
     4561 ctgcatccgg cggaaaacta cgacgagtgc caatggcgcg agctgccgcg cgccagcggc
     4621 ggcgagtttt catttcgtct gccggcggac ctcgccgggt gtgctctaac gacagttcga
     4681 ttttcggcgg acgctttggg cgccagtcgc gagctcacgt tgacgctcgt gccggccgcc
     4741 ggggagcagc cctgatggtg cggatcctcg gcatatcggc cttctatcac gactcggccg
     4801 cggccctgct ggaagacggg cagatcgtcg gcgcagccca ggaagaacgc ttcactcgca
     4861 tcaagggcga tgcctcgttt ccccaccggg ccatcggctg gtgcctggac caggccggcc
     4921 tcggcgcggg cgacgtcgat cacgtcgtgt tctacgagca gccggtggtg aagttcgagc
     4981 ggctgctggt cagctacctg gcgaccgcgc cccgcggctg ggcgagtttc ctgcgctcga
     5041 tgccggcctg gctgaccagc aagctctggc tggagcgcga gattgcccgc gagctgggca
     5101 tccgcaagcg ggtctggttc acgacgcacc atatgtcgca cgcagccagc gcgttctttc
     5161 cgtcgcccta cgaggaagcg gcaatcctga cgatcgacgg cgtcggcgaa tggtccacgg
     5221 caacctgggg cgtcggccac ggcaatcgga tcgaactggc cgaggaaatg cggttcccca
     5281 attcgctggg gctcttgtac tcggcgttca cctattattg cggcttcaag atcaacagcg
     5341 gcgagtacaa gctgatgggg cttgcgccgt atggtcagcc aaaatacgcc gcggagattc
     5401 tcgacaagct cgtgtcgctg cacgacgacg gcagcctgct gctcaaccag gcgtacttca
     5461 actatgccgg cggcctgacc atgaccaacg agcggtttca tcggctgctg ggcggcccgc
     5521 cgcggccgcc ggagtcgctc ctgacgcagc gcgagatgga cctcgcggcc agcgtgcaag
     5581 aggtcgtgaa caaggcgatt ttgcggatgg gccgctacgt tcacgagcgc acgggcctgc
     5641 cgcatctgac gctggccggc ggcgtggccc tgaattgcgt cgccacggga aaactctcgc
     5701 tggaaggccc gttcgagtcc ctctggacgc agcccgcggc cggcgacgcc ggcggagcgc
     5761 tcggcgcggc gctctggttc tggcatcacc gccttgcggc gccgcgagtt gcgccgcagc
     5821 ccgacggcat gcacggggcg ttcctgggtc cggagattcc gcccgaatcg gccgacgacg
     5881 acgcactgct ccgccggctg ggcgctgtct gggacgaatt gcagggcgac gagctggccg
     5941 agcggatcgc ggccgagatc gaggccggca aggtcgtcgc ggttgcccgc gggaagatgg
     6001 aattcggccc gcgggcgctc ggggcgcggt cgattcttgg cgacgcccgc tcgccgtcga
     6061 tgcagtcgca catgaacctg aagatcaagt ttcgcgaagg cttccgcccg ttcgcgccga
     6121 tggtgctggc cgaagacgcc gagcggtatt ttggcgcacg ccaggaaagt ccgtacatgc
     6181 tgctggtcta tccggtgagc gagcacttgc ggcggccggt gcaggcggac ggcaagttcg
     6241 gcatcgaact gctccagcag ccgcgatcgg agattcccgc ggtgacgcac gtcgatttct
     6301 ccgcccgcgt gcagacggtc gatcggcagc gcaatccgct gctggagagc gtgctccgcc
     6361 gctttaaggg ccgcacggga tgcagcgtaa tcgtcaacac ctcgttcaac gtccgcggcg
     6421 agccgatcgt caatacggcc gaagacgcct accgctgctt catggccacc gacatcgact
     6481 gcctggtcgt cggcaatcgc tttctcgccc gcgaccgaca gcagaacgcc cccctgaatc
     6541 aagatgagcg ccgccgctgg ctccagcgtt tcgacctcga ctgaaggccg cccgcgcgca
     6601 ccggacgccg ccgcgccgcg cccgcgcgcc cgcggcgctt acgcggccga cctggcccgc
     6661 cggcgcgagg ccgcagcgat caagcggcag atcggctatg gcctcgtcgt cggctggatt
     6721 ctcgcactgg tcggcggctt ctttttctac acagtgacca gcccggtcga ttggctatgg
     6781 ggcagcctga tggtcgtcgg ctggttgcac ctggttctgg ccgtcgtgct gccgcaggcg
     6841 ctctactggc ccgagcgagc ctggagcggg ctggcccagt ggcaaggcaa gctggtcatg
     6901 gccgtgctgc tcacggtcgt ctatttcggg ctgatctggc cggcccgctg gctgcggcgg
     6961 agcgccgaac cgggcttctt cgcgtgggac aacgagcggc ctggctccgg atcggcctgg
     7021 cagccgctgg acagcgcgga agcagacccg aagttagtgc acgagcgcca gcggagtctg
     7081 ccgctgctct tggcgggagt gattgcgttc ttcttccgcc ggggacactt tatcctgctg
     7141 ccgatcctga tcttgctctt ggtgttgggt ctggtgctgt tcttcgtgca gagttcggcg
     7201 ctggcgccgt tcatttacac gttgttctaa gtcggtagga cggcccggaa gggccatcct
     7261 acgttgcttc gctgctgacg ggctcttcat cccacgggtt gcgtttcttg cgctgctcgg
     7321 gctgcgggtg caactggcgc tcgtcctcga ggacccgctg cggagcttgc tgccgcacgt
     7381 gctcgacctc gcccgcggcg acgagcaagt tgagcaggaa gatcgcgccg ccgacgctac
     7441 cgatgatgat ggccagctcc cagcccccga agctgctgcg ctcggcaatc tcgtacagcg
     7501 tccagaaata gttaggcagc tggagcatgg tgtagccgaa cgtcgtatag tcgccccagc
     7561 tgatgatcgt ttgcaggagc agcggaaaga gcacgccgcc ggaggcgacg atcacctggc
     7621 agaggaacgt cgccaacagg ccgacgggcg ttacgcggcg cagcgcgacg atcgccagcc
     7681 gcacgatgcc gatataggcc aaaacataac cggtgaggct gatcgtgtag cagaaccact
     7741 cggtgaagct gggcacttcc tggccgagga tcgagccgta gagcacggcg aaccaggcca
     7801 cgctcatcac ggagagcagg ttgagcatgg caaacacgta gccggtgccc gagccgggat
     7861 tgaaccaggt gaagagcgtg cggccgagaa agctctgcgg gagctgccgc ttggctcgcg
     7921 gcgagaggtg cgccatttcg ccgctgagga acgccccggc gatcgcccag tagatcgcag
     7981 cgacggtcgt ccagacgtag aacccttcct cgacacggta ggcaacccag aagaaaatca
     8041 tccagcccac gagcaggaac tgcggcacga tcaggaccat gcggatgggc gtcgagcggt
     8101 tctcgctggc gaaggtgatc tgcccacccg agatgaacaa gagcagcgtc gccagcggcg
     8161 catagaagct gagcatgcaa aggttggcga tccagaattc gacctggtcc agcggcatcg
     8221 ccgcccggct ggccagcagg ctgacgaacg acatgtccca cacgaacgtg aacacgagca
     8281 gggccatcac gaacacgacg ctgagcagca cctgccagtg ccgggcccgc gtgacggtcg
     8341 ccatcatcag gccgaagatg ctgagcaaga gcgacgccag gaacgtgtac gcgagaaaca
     8401 gtccaatcgt gatgatgtcg atcccgcgga gcagataggt gaaggcgatg cacggcgcca
     8461 gggccgagta atagaccatc atttgcagga tcgcgctgcc cagcttgccc aggacgatct
     8521 gccggctgct gagggcggtg atcgagagca gctcgaacgt gccgtcctcg cgctcggccg
     8581 ccagcgaccg gaaggccgcg taaggcacga cgatgagcag cgggatcgat agcacgacgt
     8641 agtagcccat caaaacgccc gggccgaccg gcgcgtagaa gatcgccggc acgccgaacg
     8701 agatgaagag caccgtccac agccagccga ataccagcag cagcgagaac gtgaccacga
     8761 attgccggct cttcatcgac tggcgggctt ctttcaccag aatcggattc agccggtccc
     8821 ccgcgcggtc gagcagcgtc tcgaattgca gccaggtcgc tgcccagcgg tcggcggggg
     8881 ctgcgggagg cgcggtggcc tcggcttctt tgggcgcggc ggcgggaaga tcgacggtgg
     8941 tcatcatttc ctcactgcac cgctccgcgg gtcacggcca taaatacgtc ctccagcgac
     9001 ttgttggtgc tgccgaactc ggccacggcg aagttggcca gcacgatctc gcgcagcaag
     9061 gcgacctcgg tgtcctgctc gccttcgtgc gtgaagcgga ccagctcgcc gtcggccttg
     9121 acgtgcgtca cgtcgctgcg ctggcccagc cacagagcca gagccgccgc gccgcccagc
     9181 acacggacct tcacctcgcg gtgcggctgc tgctgctttt ggatttcggc gacggtgccg
     9241 accgccaaca gctgcccgcg ctcgatgatg cccacctcgt cgcacatttc ggccagttca
     9301 gtgaggatgt gcgagctgac cagcaccgtc ttgccgtcgg cggcaagcag gcggatcatc
     9361 tcgcgcagct cgatgcgggc ccgcggatcg aggccgttgg ccggctcgtc gaggatcaag
     9421 acgctgggat cgtggatcat cgcgcggccc aggcagagcc gctgccgcat gcccttggag
     9481 aggccgcgga tcggcttctc ggccagctcg tccagccgcg tgaaactcat tgtgtggcgg
     9541 acggcctggt gtcgttcgcg gccaaccagt ccgtagctgc gggcgaagaa gtcgaggtat
     9601 tcgtagcagt tcacgtcggc gtaggtgccg aaatagtcgg gcatgaatcc caagcggcgg
     9661 cggacgcggt cgggatcgtt cacgcacgag aagccgtcca caaaggcgtc gcccgccgtc
     9721 ggcaggtcga gcgtcgccag aattcgcatg ctcgtcgtct tgcccgcgcc gttggggccg
     9781 atatagccga acacctgtcc gcggccgacc tcgaacgtca cgtcgctcac ggcccgcgtg
     9841 gagccgaaaa agcggtgcag ccgccgcagc tcgatcatgg aatgcgtcgc gaccatgctt
     9901 caatattttc ctcgaatcac gtgcaggctg gcctcctcgc ggacgcgcgg cacgccgatg
     9961 ggcaccgcgg gggaagcgtc gacgacggcc gtgtagctgc cgggctcgag cggccgtttc
    10021 gacggcagga tgcactcgac gatgttcttc tcgagcaggc tcgccgccat cagcggcttg
    10081 gcggcgctgg catcgacgtt gttccaggct ccaccatagt tgccgttcag gaacgacagg
    10141 gcgttgttgt tgtggatgtt cgggtcgtac ccacggggca aatcgggctc gaccgcatcg
    10201 gccagcttgc ggagctcctt ctcggccgcc gccggatcga ttttggccag tttcgccttc
    10261 gcgtcctggc cgaccatttt gccggcgaag taatctccgc ggctgtcgcg cagcagcaga
    10321 tagcgaatct ccgattccag gcgattctct atttccggcg gctggccggc cgccgcaccc
    10381 tctttcacga ccagcagcgc cggcgtcttc gtcgcccgct ggatcatgaa ttgcgtggcc
    10441 gtccgcgaag tcaggtatcc ggaagtgagg tgctggtctt cgtcccagac cagcttgccg
    10501 gtagtgccgt tagggttgct gccgcgatag ccaggctcgt attcaagggg aaacaccgtc
    10561 gtgtcgttgg gataggtcag accctgcgac ggcgcgatcg cggcataata cgactgccgc
    10621 gaccaggccg cggcctcgcc cgtgcgctgg tcgagggccg tgaagctgcg cgaccgcaac
    10681 cgcacgccca ggccgtcggt taacagggcg tacgtgaaga ggcacagcgt cacgagcagc
    10741 gccccggcag gcacggtgag cagcagcaga tagagccgcc gcgagcgtcc aagcaggacg
    10801 taattgacgg gcccgatcac gatcgcaaac agcgagacca gcagcaggaa cgacacgacg
    10861 ggggcttcgc cgacgccagg aatcaggaac gtccaatagt cgtcgttcgt gcgatgctgc
    10921 gagaagccag tgcggcggaa ccatttccag tggttgtccg gcaccgagtt gaatagcagg
    10981 atccaatcgg tgtcctgtcc gggaaacggc ttggcggcgg agatggccgc gacgcagccg
    11041 acgcccgccc gccggaacag aaacggcggc ttctccagca gcggactatc gtccttcgac
    11101 ttggcgtcct tcgacttggg cgcgatcgcg gccgtcggct tgcgcttgtt gcggattgtt
    11161 ttcgaatagc ctccgccggg atactgctgg gtgagctcgt agtcctgcgc ttcccacgcc
    11221 gaacgcaact cgtgcttctt catgttcgta tccggcgcgg tccagccgcg atgctcggca
    11281 ggctgctctt cgccagccgg cagcggcggc agctttagca gctgctccag atcggccagc
    11341 cgctcgaact tgtccccgac gtcatacaca ttgagcagcg ggcctgtgga cagccattgc
    11401 cgcagggctg cgaacgtctg cggctgtttg gcggccaggt ccttcaggtc gccgaagcgg
    11461 atcgtgatga cgtcgtactg gctgaggtcg atccagcgct cgggcaattc cgccggcggc
    11521 agcatctcga cgcggcccat gtcggcgatt tgcgacagca gcatgacgtc ggtcggcttg
    11581 ggcgtcgtcg ccaaaagcat gccgggcgag gtccccggag agccgacggg catcccgcgg
    11641 ctgggatcgg gaaacaggta ggccatcgtg cggacgtcgg gcaggttgtt cgtcggcgtc
    11701 gggtcggcgc tggtcgattt gaaggcagcg acggcaggat cacgagcggc gcgcgccaga
    11761 acctgcgaat cgatgaacag catcgtcggg cggtcttcgg tccagtccca atagttgacg
    11821 ttggcccagc ccatgtaggg atgcgacagg tcctcgagct tctcgccccc ctcgtaggtc
    11881 tcgatcgcca tcgaatacca gagggccgat tgcggcaccg agaggatggc ttcgacttgc
    11941 atgctgcctt cgggcaagtc gatcacctgc gaaacctgcg gagtgctcgc cgcgttgtac
    12001 gaattcggct tgatgacgat gcgaatctgc cggtcggccg tcagcggttt gggaggccag
    12061 ggggtgactt cgacgcggac gggccggtag ccgttgccct cgacgccgcg gccgtcgagc
    12121 gagatgatca ggccactttt gttcttgacg cccacttgcg gcagcgaaaa cagcggcccg
    12181 ctgccggcgc gcgcggccgt cgccaatagc ccgcaaatca gcgcggcggc cagcgctgca
    12241 aatagacccg ggaatcgggt gcggcggacc atcttcggct cctgggcgga ctatccggta
    12301 atgggaggcg gaccttcggt cattggcgat tcaaccaggc cagcgccctc gaagggcgcc
    12361 tcagccacgg tcttgttggc aaacggcgat tgaccctcgg cgcggcggcc gaacaggccg
    12421 attgacatct gggaaacaag ccaggccccc aggaacgctg ccacgtgcag gatcgccacc
    12481 gccgcaacgc tgcccagcgc ggccgccgac ccgaccgccc agccgtcgcc ccgcatggcg
    12541 aacgagagga tgagcgaaat tcctgcgcac agagccgtca gccccaagag ccaccgcagc
    12601 gagaattgcg gaatgatcat gcggtcgatt gttgcacacg agtgcggcaa ttgccaagcg
    12661 acgcagaaat caacccgaag cgtaagcgag ggacattatc caggcacgtc ggattccgat
    12721 ccctcgtttg cacttccggt tgctatgccg ctaccgtatt cgccagcatc cgcgcgatat
    12781 tgagacgatt cgtctgattt tactcggaac cgcgggatgg tgcggatatt ccccgcagcg
    12841 gctgccattt tcgttcggcg acgcggcgga tagattagca attggcctac atgtgcagcc
    12901 gacgtcgctg ggcctggttc tgctcccgcg ccgcctccgc cgttgtccga cctacccgcc
    12961 ccaccagaat cgccccagaa ttccaactgg gccctcatcc gccgcatgat cggcctgggg
    13021 tggcagtatc gctggggctg cctgcggctg ctttgcttgc aggggctgct gctgctcgcg
    13081 gcgctgggca cgctgcgact gacggggctg ggcatcgacc tgattcgctt ctacgcgcat
    13141 gcctcgctcg acaagccgcc gattccgttt ggcctcactt tgccgttttc gtcgccgctg
    13201 gcccaggtgg ccctgctggc ggcgctggtg ttgctgctag aggccattcg cggctcgctg
    13261 aactacgtgt atgccctctc ggcgggctat ctggtacaca cgcggatcgt gccgcagctc
    13321 cgcagcctcg tttacgacaa gctgcagcgg ctctcgttcc ggttcttcga cgccaacgcg
    13381 accggctcga tcatcaaccg ggtgaccagc gacgtgcaga gcgtgcgctc gttcatcgac
    13441 ggcgtgctga ttcagttcgt gctgctcttg gtctcgctgg tctgctacct gggctacatg
    13501 ctcagcctga atgtcggcct gacgctggcc tgtctggcca cgacgccgct gatgtggctg
    13561 gtgaccgtga tcttctcgcg gatggtccgc ccgctgtacg accgcaaccg cgacctggtc
    13621 gatcgcgtca tcctgcggct ggccgagagc attcagggcg tgcaggtgat caagggcttt
    13681 ggcagggagc gggaggagat cgcccgtttc gcggccgaca accgcgccgt ccgcgaccag
    13741 cagcacggca tcttctggcg ggtcagcatt ttcggcccca cgatcggcta catgacccag
    13801 atcaacctcg tgatcctgtt ggcctacggc ggctggctgg tcacgcagga taggctcgcg
    13861 ctgggcagcg gcctggtcgt cttcgcgggc ttgctccagc aattctccag ccaggttgcc
    13921 aacctgacga acatcgccaa cagcgtgcag cagagcctgt ccggcgcgcg ccgcgtcttt
    13981 gaaatcctcg acacgccgat cgcgatccac agcccgccca acgcggtgag acttgtagcc
    14041 cgcgactccg tcgcgggaac ccgccacgga gtggcgggct acggaggccg catcgagttc
    14101 gaccatgtct ggttcgagta cacgccgggc aacccggtgc tgaaagacgt gacgtttatc
    14161 gtcgagcctg gccagaaggt cgcgattctg ggtgccaccg gcgcgggcaa gacgacgctg
    14221 ctggcgctgc tctgccgctt ctacgacccg acccgcgggc gaatcctgct cgatgggcac
    14281 gacctgcgcg acctggacct cgacgacctg cggcgacata tcgggctcgt ctttcaggag
    14341 acgtatctct tcagcaacac ggtggccgcg aatatcgcct tcggccatcc cgaggccacg
    14401 ctcggccagg tccaggcttc cgcgaagatc gccgcggcgc acgatttcat cgccgcgctg
    14461 acgcacggtt acgacacggt tctgggtgaa ggcggcctgg acctttcggg cggccagcgg
    14521 cagcggctgg cgatcgcccg cgcggtcgtg ctcgatccaa cgctgctctt gctcgacgat
    14581 ccggccgccg ccatcgatcc gcatacggag cacgagatcc tctcggcgat ggagcgggcc
    14641 atgcaggacc gcaccacgct cgtcatcgcc caccgcctga gcacgctccg ccagtgcgac
    14701 cgcgtcatcg tgctcgagca cggccgcatc gcccagcgcg gcacccacgc ggagctgatg
    14761 gaagccgacg gccactatcg ccacgccgcc gagtcgcaac tcgccggcgc gcgggcgtga
    14821 gcgactggct cagactataa ttaacagccg actccttctt cgctccaaac gcgctaccat
    14881 gaccgt
//
LOCUS       DASRJB010000558         5615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1568071, whole genome shotgun sequence.
ACCESSION   DASRJB010000558 DASRJB010000000
VERSION     DASRJB010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568071"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..840
                     /locus_tag="VFB80_14520"
     CDS             301..840
                     /locus_tag="VFB80_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HZN35039.1"
                     /translation="MLSVRPKVAELSFQLFGRALHPELFEVHKTRTVERGEFSARLDI
                     TSAGHVVTWRYRGMTLTEVAASAQHPLPERRRLLSYRLKGQRSDRVECRGGVVYQTSF
                     QLEPVAPELFWTFQEELAQGGERQGLIHTFDSSGRMALGAVSYIYAETRTRSLLVQAF
                     HTFPDDCAIVKCQSVFELP"
     gene            complement(844..1635)
                     /locus_tag="VFB80_14525"
     CDS             complement(844..1635)
                     /locus_tag="VFB80_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN35040.1"
                     /translation="MPAVMDSDFRLNYEERGSGPPLLLVHGFPLDHTMWRFQLEALSD
                     RWRVIAPDLRGFGQSSVAPGTVTMAQFADDLAGLLSVLKIDRPVVFCGLSMGGYVAWQ
                     FALKHRSKLAKLILCDTRAIGDSAEAAAGRLKTAEKVLAEGARVVADAMLPKLFAAST
                     ATRSPALVAATKEVILKTSPAGIAAALRGMAERPDVTSRLSELDLPVLVICGQHDAIS
                     PPAEMQGIAAKLPQGKYVEIAEAGHMAPLEQPAAVNRAIAEFLGP"
     gene            complement(1742..2215)
                     /locus_tag="VFB80_14530"
     CDS             complement(1742..2215)
                     /locus_tag="VFB80_14530"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HZN35041.1"
                     /translation="MSAADGAAPLVARYAGPRPDPMYHVVLLAMCSGVLVLAAVLSVR
                     NQSQVVLPILGLPLPELCLARRLTGWGCPGCGMTRCFIALMHGDWLAAWRYNPSGIWL
                     FGLMAAQIPFRAIQLWRIRRGLPELNTGWTAQILLGLLIVLMLGQWVLRAIGLPI"
     gene            complement(2212..4083)
                     /locus_tag="VFB80_14535"
     CDS             complement(2212..4083)
                     /locus_tag="VFB80_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35042.1"
                     /translation="MNLASFRTWMLGLALAAVLAGGPAGRALADDPPADPAAQDAKLK
                     TLIEQLGADGDFAARERAQAELAKLGLEAFDALHAAQNHHVPEIALRARYLVRSMNVR
                     WFSETDGPEVIRILKEYGDLSESDRRNRMERLAALENRQGVAALVRLSRFETNDPLAK
                     YAALKILEMPPPADDAAKAETAKSIRNIVGTSKRPAAGWLLLYARSLADPAATLAEWD
                     QATKAEHEALKNPDKTSREIVRDLYRYQVDTLKRLSRNEEAIAVIRRTFDLTNEVPDH
                     VLEIVDWLMQIQAWPVVLEAMQRFEKTVADNADLLYRQAETYQRLGRTEEAEVTAKKA
                     LALRPENLEEHLLIGFGLEEKRGMHAWAEREYREVIKAAAAGSVADFKARFRLSELLH
                     DQAQELLAAEALKPVCDLMQKDEAAKEACQRAYRSPEGVIARMHFFFACHAHGQKDYP
                     KEREHLDLAITSDPTDADALIAMYRVPDADNPWKEKTRKAIEETSDMYAKGIDEWQQR
                     YDAADNEQIQSAAGEQLAQECNQYAWLVGNTIGDYDKAVRMSHKSLELRPSTYSFADT
                     LGRAYYGKGDVANAVKYQRLAVKLSPFSGQIRRQLDFFVKEAQAKGVKLPPAEGTQP"
     gene            4280..4885
                     /locus_tag="VFB80_14540"
     CDS             4280..4885
                     /locus_tag="VFB80_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN35043.1"
                     /translation="MNLTASLLAITAKMLSGATLRWHECQPDTCQRVYFANHTSHLDA
                     LVLWATLPGDIRDLTRPVAAKDYWAQGAVRRYIARSFNAMLIDREEIKVHQSPVDMMI
                     REIGMTHSLIVFPEGRRNTEEEMLEFKSGLYYLSKKRPDLELMPVYIDNLNRVLPRGE
                     FLPVPLLSCITVGAPIWLEPNESKLDFLKRARQAVLRLKDV"
     gene            4889..>5615
                     /locus_tag="VFB80_14545"
     CDS             4889..>5615
                     /locus_tag="VFB80_14545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN35044.1"
                     /translation="MTPAELLPLLAQLGPNVRLDPRTFILMFSVVAALLVAYFVGRTL
                     RRQPESTVHPAIVQTFNQRVAAWLFMFCILFAALIPQGRVAYICTIVLFGFVSFWALR
                     EFITMTPTRRGDHRTLFLTFFVFTPMQYFVVALGRNYYGLYTLMIPVYASLLIPAMIS
                     ISGDPKRFLERVAKIQAGLMICVYCLSHAPAMLDLDLKTSRGGQPWFIDGPAPAPAAQ
                     APAAAPAAPAAPPAPAAPRARGSN"
BASE COUNT          875 a         1959 c         1783 g          998 t
ORIGIN      
        1 tgcagtgcta gcgtgccgcg cttcgtgcag catctcccac gcagtttagt acggcagacg
       61 gcggacgttg gtcaaggcgg atttgcctgc gattcagcgg cgcggcgggc gccacgccca
      121 cgcttcgtgg cgtgggggtg gcactcagga gcggccgatt cacaccctgt caagaggcgt
      181 gcccgcgaca aattcggccg cgggcaaact ggctttgtga cctccaaatg ggcgtcgtat
      241 aatgcgaccc agcatgaggc ttttccagtt acttgcagct cctcacgcgg aatcatcgcc
      301 gtgttatccg ttcgaccgaa agtcgccgag ttgtcgtttc agctcttcgg ccgcgcgctg
      361 caccccgaat tgttcgaggt gcacaagacg cggaccgtgg agcgcggcga gttctccgcg
      421 cggctcgaca tcacgtcggc cgggcacgtc gtcacctggc ggtatcgcgg catgaccctc
      481 accgaggtcg ccgcctcggc ccagcacccg ctgcccgaac gccgccggct gctctcctac
      541 cgcctgaagg gtcagcggag cgaccgcgtc gaatgccgcg gcggcgtcgt ctatcagacg
      601 agctttcagc tggagccggt cgcaccggaa ttgttctgga cgtttcagga agagctggcc
      661 caagggggcg agcggcaggg cctcatccac acgttcgatt ccagcgggcg gatggccctc
      721 ggcgcggtca gctatatcta cgccgaaacg cgaacgcgga gcctcttggt gcaggccttc
      781 cacacgttcc ccgacgactg cgcgatcgtg aagtgccagt cggtgttcga gctgccgtag
      841 caactacgga ccgagaaact cggcgatggc ccggttgacc gcggcgggct gctccagcgg
      901 cgccatgtga ccggcttcgg caatctcgac gtactttccc tgcggcagct tcgcggcgat
      961 gccctgcatc tccgccggcg gcgagatcgc gtcgtgctgg ccgcagatca ccagcaccgg
     1021 cagatcgagc tcgctcaggc ggctcgtcac gtcgggccgc tcggccatcc cgcgcaaggc
     1081 cgcggcgatt cccgctggcg acgtcttgag gatgacttcc tttgtggccg cgaccagcgc
     1141 cgggctcctg gtcgccgtgc tggcagcaaa cagtttgggc agcatggcgt cggccacgac
     1201 ccgcgcccct tcggccagca ccttttcggc cgtcttcagc cggccggccg ccgcctcggc
     1261 cgaatcgccg atggcccgcg tgtcgcacag gatcagcttc gccagtttgc tgcggtgctt
     1321 gagcgcgaat tgccaggcca catagccccc catcgacagg ccgcaaaaga cgaccggccg
     1381 atcgatcttt agcacgctca gcaagcccgc cagatcgtcc gcgaattgtg ccatggtcac
     1441 cgtaccgggc gcgacgctgc tttgtccgaa gccacggagg tccggcgcaa tgacgcgcca
     1501 ccgatcggac agcgcttcca gctgaaagcg ccacatcgtg tggtcgagcg ggaagccgtg
     1561 gacgagcaac agcggcggcc cgctgccccg ttcttcgtag ttcaggcgaa aatcactatc
     1621 catgacggcg ggcatgggag cgtccttgta ggtcccgcct gccgggcggg acctgggcag
     1681 agcgttgagg ttcccggtcg ctgcgcttcc ctcaaggccc cttccggcag aaggggccta
     1741 cttagatcgg caggccgatc gcccgcagca cccactggcc gagcatgagc acgatcaaga
     1801 gtcccagcaa aatctgggcc gtccagccag tgttcagctc cggcagcccg cggcgaatcc
     1861 gccacagctg aattgcccga aacggaatct gcgcggccat caagccgaac agccagatgc
     1921 ccgacgggtt gtaccgccag gcggccagcc agtcgccgtg catcagggcg atgaagcacc
     1981 gcgtcatccc gcatccaggg cagccccagc cggtcagccg gcgggccagg cagagctccg
     2041 gcagcggcag accgaggatc ggcagcacga cctgcgattg attccggacc gacagcacgg
     2101 cagccaggac gaggaccccg ctgcacatcg ctagcaggac cacgtgatac atcgggtcgg
     2161 gccgcggccc ggcgtatctg gccacgagcg gcgcagcgcc gtccgccgcg ctcacggctg
     2221 cgtcccttcg gccggcggca gcttgactcc cttggcctgg gcctctttga cgaagaagtc
     2281 gagctgccgg cgaatctggc ccgagaaagg actgagcttg accgccagcc gctgatactt
     2341 cacggcattg gccacgtcgc ctttgccgta ataggcccgc cccagcgtgt cggcgaagct
     2401 gtaggtgctg ggccgcagct ccagcgactt gtggctcatc cgcaccgcct tgtcgtaatc
     2461 gccgatcgtg ttgcccacga gccaggcgta ctggttgcat tcctgggcca actgctcgcc
     2521 ggccgcggac tggatctgtt cgttatccgc ggcgtcgtac cgctgctgcc attcgtcgat
     2581 gcccttggca tacatgtccg acgtctcctc gatggccttc cgcgtcttct ctttccaggg
     2641 attgtcggcg tccggcacgc gatacatggc gatcagcgca tcggcgtcag tcggatcgga
     2701 agtaatggcc agatcgaggt gctcgcgttc cttgggatag tccttctgtc cgtgggcatg
     2761 gcaggcgaag aagaagtgca ttcgcgcgat gacgccttcc ggcgagcggt aggcccgctg
     2821 gcaggcctcc ttggccgctt cgtccttctg catcaggtcg cagacgggct tgagcgcctc
     2881 ggccgccagc agctcttggg cctggtcgtg caacagctcg gagaggcgga agcgggcctt
     2941 gaagtcggcg accgagccgg ccgcggcggc cttgatgacc tcgcgatact cacgctcggc
     3001 ccaggcgtgc atgccgcgct tctcttccag gccgaagccg atcagcaggt gctcttccag
     3061 gttctccggc cgcagggcga gggctttttt ggccgtgacc tcggcctcct cggtccgacc
     3121 gagcctctga tacgtctcgg cctgccggta gagcagatcg gcattgtccg cgacggtctt
     3181 ctcgaatcgc tgcatcgcct ccagcaccac cggccaggcc tggatctgca tcagccaatc
     3241 gacgatctcc aggacgtggt cgggcacttc gttggtcagg tcgaaggtgc ggcggatgac
     3301 cgcgatcgcc tcttcgttcc gcgacaagcg cttcagcgta tcgacctggt agcggtacaa
     3361 gtcgcgaacg atctcgcggc tggttttgtc gggattcttc agggcttcgt gttcggcctt
     3421 cgttgcctgg tcccactcgg ccagtgtcgc cgcgggatcg gccagcgagc gggcatagag
     3481 cagcagccag ccggcggcgg ggcgtttgct ggtgccgacg atgttgcgga tggacttggc
     3541 ggtctcggcc tttgctgcgt cgtcggccgg gggcggcatc tccaggattt tgagcgccgc
     3601 gtacttggcc agcggatcgt tcgtctcgaa ccgcgacagg cggaccaggg cggcgactcc
     3661 ctgccggttt tcgagggctg ccaggcgctc catgcggttg cggcggtcgg actcggacag
     3721 atcgccgtat tccttgagga tgcggatcac ttccggcccg tcggtctccg aaaaccagcg
     3781 gacgttcatg ctgcggacca ggtaccgcgc ccgcagggca atttccggca cgtggtggtt
     3841 ctgcgcggcg tgcagggcgt cgaaggcttc caggccgagc ttggccagct ccgcctgggc
     3901 ccgctcgcgg gcggcgaaat cgccgtcggc cccgagctgc tcgatcagcg tcttcagctt
     3961 cgcatcttgc gcggccggat cggccggcgg atcgtcggcc agggcccggc cggccggccc
     4021 gccagcgagc acggcggcaa gcgccaggcc gagcatccac gtacgaaagg aagccagatt
     4081 catgcccact acctcccagg ccacgctaca accccttcag cttacccctg cgccccggca
     4141 gcggacagag cgattcgcac ggccgccggc tgcgattgcc gctccgcggg ccgcgggcta
     4201 cgcttacttt cgtccggtca tagttgacct cacgctccgc gtgaggcaaa catctcgcgg
     4261 agcgagatgg ctactctcga tgaacctcac tgcctcgctc ctggccatca ccgccaaaat
     4321 gctcagtggg gcgacgctgc gctggcacga atgccaaccc gacacctgcc agcgggtcta
     4381 tttcgccaac cacaccagcc acctcgacgc cctcgtcttg tgggccacgc tgcccggcga
     4441 catccgcgac ctgacccgcc cggtggccgc caaggactac tgggcccagg gcgccgtccg
     4501 ccgctacatc gcccgctcgt tcaacgcgat gctgatcgac cgcgaagaga tcaaggtcca
     4561 ccagagcccg gtcgatatga tgatccgcga gatcggcatg acgcactcgc tgatcgtctt
     4621 tcccgagggc cgccgcaaca ccgaggagga gatgctcgaa ttcaaatcgg gcctctacta
     4681 cctcagcaag aagcggccgg acctggagct gatgcccgtc tatatcgaca acctcaaccg
     4741 cgtgctgccg cggggcgagt tcctgccggt gccgctgctc agctgcatca ccgtcggcgc
     4801 gccgatctgg ctggagccca acgaaagcaa gctcgacttc ctcaagcggg cccgccaggc
     4861 cgtgctgcgg ctcaaggacg tgtgacggat gacgccagcc gaactattac cgcttctcgc
     4921 ccagctcggg ccgaacgtcc gcctcgatcc gcggacgttc atcctgatgt tctcggtcgt
     4981 cgcggcgctg ctggtggcct atttcgtcgg ccgcacgctc cgccgccagc cggagagcac
     5041 cgtgcacccg gcgatcgtgc agacgttcaa ccagcgagtc gccgcctggc tgttcatgtt
     5101 ctgcatcctg ttcgccgccc tgattccgca gggccgcgtg gcctacatct gcacgatcgt
     5161 gctgttcggc ttcgtctcgt tctgggcgct ccgcgaattc atcacgatga cgccgacccg
     5221 ccgcggcgac caccgcacgc tgtttctgac gttcttcgtc ttcacgccga tgcagtactt
     5281 cgtcgtggcc ctgggccgca actattacgg cctctacacg ctgatgatcc cggtctacgc
     5341 ctcgctgctg atcccggcga tgatctccat ctcgggcgat cccaagcggt tcctggagcg
     5401 ggtcgccaag attcaggccg ggctgatgat ttgcgtctat tgcctcagcc acgccccggc
     5461 gatgctggat ctcgatctga agaccagccg cggcggccag ccctggttca tcgacgggcc
     5521 cgctcccgcc cccgccgccc aggcccccgc ggctgcgccc gccgcgcccg ccgctccgcc
     5581 agcgcccgcc gcgccgcggg cccgcggcag caacg
//
LOCUS       DASRJB010000559        15917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3822253, whole genome shotgun sequence.
ACCESSION   DASRJB010000559 DASRJB010000000
VERSION     DASRJB010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3822253"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..597)
                     /locus_tag="VFB80_14550"
     CDS             complement(268..597)
                     /locus_tag="VFB80_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZN35045.1"
                     /translation="MSKTRQKVFEYVEPIGARVLVRKDDAKRHTKGGIALPDAAEIPT
                     ITGRIVAISAQIENDEDLPLRQYDKILFHPKNAIPVDFESDNQLFVIPVEDIVAVFRR
                     EEKESSE"
     gene            919..2586
                     /locus_tag="VFB80_14555"
     CDS             919..2586
                     /locus_tag="VFB80_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35046.1"
                     /translation="MRKLLLTLAAISLGLVAGPAATLAQNAGQEDLDKATELQITAET
                     LGELEQVIKLAETALDKGLDKGQTEFAKKLLGATLFQHAERSARSVFEQIPPSRNWRS
                     IRDQGMKNLAKAKKFDPTLPDVYLLEAKFQALPGGETRDAIAALDEAIKLLKPKDQPK
                     QLADAYVLRAQFLEDDERKLADYDEALKVQPKHVAARQARALHYLTKGENEKAIADFQ
                     KLLEQDADNPGVIAELARALILNKKFDEAMKYCEEVIKLAPKATEGYKLRAQLRWIKE
                     DTKGALEDLGEALSINPNDISGLLMRCRLYASEKKDALAKADLDRVLRLRPDLPQAIE
                     LRSTMAALKQNYADAIADIQTLLQGDPTNPELRLQLASYYVGDKRPRKAIELITSVID
                     GLADDKDAGAKENKADALRQRGDALLSVGKHADAVKDYEEALKLDPNDTGVLNNLAWV
                     LATSPDDPVRNAKRSLECSLKACELTKYEKPHILSTLAAGYAEQGDWETAIKWSGKAV
                     ELGVEEVMVDDQLKKELQSYKDKKPWREKQEVEENTKPLGKKSDLET"
     gene            2586..3155
                     /locus_tag="VFB80_14560"
     CDS             2586..3155
                     /locus_tag="VFB80_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN35047.1"
                     /translation="MDRSAALALVHEYTPSLSLRRHMLAVEAALRAYARKFGEDEEKW
                     GLVGLLHDFDYERWPNPPDHPLQGAAILAERGYPADVIYAIKSHADYLPDCPRVSRLD
                     KTLYACDELAGFITACAMVRPERLAGMQASSVKKKLKAKGFAASINRDDIARGAADLG
                     VDLDQHIQFVIDAMQPIAGELGLAATPAA"
     gene            complement(3251..4087)
                     /locus_tag="VFB80_14565"
     CDS             complement(3251..4087)
                     /locus_tag="VFB80_14565"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZN35048.1"
                     /translation="MNDSALGLLLLVGLAGCAAEAPPRAEAAAPAVQPAEPAAAAGRQ
                     IIRRALLVVRVESFGEAERRLNELIGADGAYVAQFREDRSSGARRGGQWTIRVPVPRF
                     AHFVEQAGLLGIAEQRETHTDDVTEEYVDLEARAKNKQALEARLLELVARRSDDIKDV
                     LALEAELARVREEIERMQARLRQLRDRVALSTVDITAYERLDYRPPLATTFAGKISQT
                     FWDSANELRKCGEACVLVIVGLLPWLAAISLALCVVLAPLVWWARRRNRRPVIVVAQA
                     VA"
     gene            complement(4509..5366)
                     /locus_tag="VFB80_14570"
     CDS             complement(4509..5366)
                     /locus_tag="VFB80_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZN35049.1"
                     /translation="MLNETSSPQPQSVFPNYLLAEDDGKFLKRPELRPVRLQLELLKP
                     DMIFEEQRVTSTLVVFGGTQIVERPEAERRLAEAKTALAADPADPLAQRAVSRAERVL
                     AKAHYYDEAREFARLVSAACQVHGQCDFAIVTGGGPGIMEAANRGAADVGAKSIGLNI
                     TLPEEQLPNPYITPDLCFQFRYFAIRKMHFLLRAKGLIVFPGGFGTLDELFEVLTLRQ
                     VNRMQPIPVILYGREFWDGVLNLRFLADEGTIRDDHLSLVSYAETPHEAWEIICQFYR
                     LPEDRYAVG"
     gene            complement(5360..6319)
                     /locus_tag="VFB80_14575"
     CDS             complement(5360..6319)
                     /locus_tag="VFB80_14575"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZN35050.1"
                     /translation="MEFDVWPAVLFAAPPIREPRVPLWVMEWTAAVLLHVWLFCVGAC
                     VGSFLNVVVYRLPRGRSLAHPGSCCPSCGHAIRLWDNIPILSWLLLRGRCRDCRAPIS
                     PRYFWVELFVATLFLAVAFWERTHFIGVWPGVPLRPLPSGAYDLWPYWSRYAVHVVLL
                     ATLVGGALILGDSFRTPSRLFWPAIGLALLLPVIWPEIRSIAAWRYGKMPDWQAGLID
                     GLAGLAAGVIGGLAGSLWRWFRTGEWPPFAPVSWAASLCIVLGWQRGLLWSVPALALY
                     LVAAGLLWTVQSFLADLAPATDSPTDRPPTTDLPTTDPPPPPC"
     gene            6581..8512
                     /locus_tag="VFB80_14580"
     CDS             6581..8512
                     /locus_tag="VFB80_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35051.1"
                     /translation="MRWIASLAAFALAALLAQPAAAQLVATAAAPRSAEADQIRGWYR
                     DYLGREVGPELTAWVELLKGGMSAIDVQATILGSDEFYYSKGRDPESWVRETLQAVTW
                     ADPTYAELRRWNDRLSRLHGDRFALSREIVLSTSQPQTQTPAGQVGDLAARLASAARL
                     AVDTIDFEIGGTTQGQQANLRAQALLSAASQLNQVVTLRSYRPDDALRALDSADRSYQ
                     ALQQTLNSLPGSAPSATGIVRRIGTMLTDARGAIRPPVVGPTYPTTPTIPGGYGFDTR
                     QLTAQVDAISRGVESLTQLLTSQAYQNYAYSALLRDLDTFASRVDAFEQALVRGTSRD
                     RLSWELESLREQGGRIRTQLLAGRPPYFARLYWQSVESGLDQLRDTLGPASTSGSPAG
                     SGVAVLRPPLYDNLVTLLDQAAQHLEVFLAGTNPLVYGIPDVPRVQRDARILKNRILE
                     LRQQAANGDPAPVLKQTLQTMVGEYKVVYDRWNQVVAGNRLINPARLSPAGETLNRVE
                     QLINDALAAGDLPAAGPTRAGQLLAALQNDVNSGRRSLVAFGGYREQQSIDTYFGQLS
                     DYVQSINQSQTRTTNVDARRLAVAMQGVVGRLQNDADALNARVDSAGTVQQRQQATAL
                     VQLAASIGRLVDEIEAALY"
     gene            8684..9949
                     /locus_tag="VFB80_14585"
     CDS             8684..9949
                     /locus_tag="VFB80_14585"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HZN35052.1"
                     /translation="MRCLLVITLLCTLALPAAAQTKPAIVARKSSEQSAANFQAVMQQ
                     ALGALARAGSYALDVQSEWGAVDDPNGPQGGSRYRLVAAGGKYRVEVRSPAAQAPELL
                     CVNDGRQVTTLLPARGLYSQHAAGSPQASLEANTMLSLSLQGSAIDILLQPDVAGHVR
                     TQASDLKDLGAGQLGSVKAHHFELVWAGAKVQLWFAAEGEPLLLQFRRTTCVPTGTSD
                     FYEQECTAKFQWQLGVKPAEGTFALALPKDARRVNEIYDALSGEESASRIGKPLPKLQ
                     LGKLDGSEIALAAAEGKKATVLIFWATWCASSTEDLATVSQFVAANKDKGIAFYAINV
                     GEQPGEVRRFTAKSPLVSTVLLDPRGKASSALRVTELPAVAILAPDNTVRAILHGTAK
                     ELQSELTAQLESLLSASTARSPGETTRPK"
     gene            complement(10064..10396)
                     /locus_tag="VFB80_14590"
     CDS             complement(10064..10396)
                     /locus_tag="VFB80_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35053.1"
                     /translation="MNLISRLTGMFSRGGRDEERLRQAMEHAKANRPERAMQIYNSLV
                     ASTTTDREVRAKALFNRALAHSSLKEDQRALEDLSRVLALPNVPDSVTTAARSQLARV
                     RKRSERRA"
     gene            complement(10564..13431)
                     /locus_tag="VFB80_14595"
     CDS             complement(10564..13431)
                     /locus_tag="VFB80_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZN35054.1"
                     /translation="MLNRLLGWLDSRTGYRRIVNEALYERIPSGARWRYITGSMLVFA
                     FVTQSVTGIFLWMAYSPSSQTAYESVYFIQHEMAGGWLVRGIHHFMAQAMVVVMALHL
                     LQVVWDGAYRAPREVNHWLGLVLLQLVLGLGLTGYLLPWDQKGYWATNVATNLATLVP
                     GVGKQVQQLAVGGNEYGHHTLTRFFAMHAGVLPVLLIAALTVHIALFRRHGIMATITP
                     GRADETFWPKQVLLDAAGCLVLLAIVLLCVFHWDLNALASGKFDPAHLGAELGAPADP
                     AEAYSAARPEWYYLFLFQLLKYFPGSSEIIGAIVIPGVVMTIFALMPFIGWSNLGHRF
                     NQAFTVLVLLAAVGLTGAALFDDYYAQVAPRLGLHDNKKLTASKEFLAARADAEIQAR
                     RSIELINRREMLPDGKLSDVRLIPKQGAVYLLRNDPLTRGPRLFGQHCAGCHDYADPK
                     GQRPPQVAGLFQSPVVGEGGKVVRSADEQVTYGDAANSAPNLYGFASREWIKGLLNHD
                     LLVRFEIGPPQPSPDPKIAADKDRPDNHKRPIDAPYFGNTGHKTGRMAAWVKEHSAKL
                     LKDDPAKSDDDLDAIVTALSAQAKLPGQAAADGKDKTLIERGVGLIELNCTKGCHKFG
                     ASGQLGLAPDLTGYGSYEWMLGLASDPAHERFYRQENDGMPSFAANLAQPEKNQLSVR
                     ELSLIVDWLRGDYYRAGDAAPRLPHPEDVAQKAVQLARTTASPWTSVVGAPQAAKPSE
                     AQQAERLFAANCAACHSHTYEHGRGIAAAWPSAPNLFGFGSRAWLAGMLDLELIQSDR
                     YFGQTRHAKGDMVGFVESDLAEPNDAAKAKVAAIVAALSAEAALPSQADEDKKAADDG
                     MLERGRKAMGEAFENQACVECHKFRDAGDVGSAPVLTGWASKDWLVRFISDPTHDDFY
                     RATNDRMPSFGKDPRGPKQPLLSPEQIELLARWLRGELSGN"
     gene            complement(13540..14145)
                     /locus_tag="VFB80_14600"
     CDS             complement(13540..14145)
                     /locus_tag="VFB80_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN35055.1"
                     /translation="MAKSLNPHAAGHAAPPPPPAAAPRRNVLVACLAAICAAVVGLFP
                     FGAGLLVFLDPILKRKQNSQGGEDQRPFRRVASAAALPADGTPVQVPVIADLTDAWNR
                     EPNQPVGAVYLRKSAQGVECFNAICPHAGCFVGYAADRKVFQCPCHTSSFQLTGQRIL
                     PSPSPRDMDALKVDEAKLQEGEVWVQFVNYYPGKPDREEKP"
     gene            complement(14326..14727)
                     /locus_tag="VFB80_14605"
     CDS             complement(14326..14727)
                     /locus_tag="VFB80_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35056.1"
                     /translation="MNVASSAVNPYASPLAQSRGEPEFSPVEFSAQLLSRGWMRRRFA
                     VSGPCPCIVDYNGRGIGYESVLVNGALAVRIHGDWRKLSDWIWLQPRLDFDLFGSTMR
                     KRACIEARGILRLSAFRLTVDGKVLYSEGTW"
     gene            complement(14724..15794)
                     /locus_tag="VFB80_14610"
     CDS             complement(14724..15794)
                     /locus_tag="VFB80_14610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35057.1"
                     /translation="MALGLTAAPALDPAAQAAVADLLLLPDEQLLTRFFKQHEDGAFA
                     AIVKRHGPVVYGVCRRILSDSNDAEDAFQATFLVLVRKGASLRQPGKLSSWLYGVANR
                     TARKVKLQAALRKSCESRAPRPLPSDPGELKYDELRSILDEEIGQLPEKYALPLVLCY
                     LEGKTNAQAAEQLGWPEGSMSRRLSRGRELLRKRLMRRGLALTAALIAAVFCKQTLAA
                     VPAALAEATAIAGQKLLAGAKLREVASPAAAKAVLAVLESMTAAVRLTGASLLAAASI
                     LLALTTAIYTFTRPAYGASSYGFGNGDWFQGLVTNSSDRSTRYVFPTVAPASGGCGGA
                     VVAPSCGDSSCAPPTAHSTPPL"
BASE COUNT         2696 a         5357 c         5470 g         2394 t
ORIGIN      
        1 ctacgatggc gaggaattgc atcgggcgaa ttcaaaggtt gacgagggcc ggaggcccgg
       61 caggtaatag ccccggccgt gaggccgggg tttgaggtga tattacaatg aacgcaggcc
      121 cggagggccg gcacattgga cgccggaatc cgtcctgcca tgtgccggcc ctccgggcct
      181 caacgtttca tatttgcgac gaataatccc cgacctgacg gtcggggcta cttcctgtcg
      241 gccctccggg cctgaggacg tggtgggcta ctccgacgat tctttttctt cgcgccggaa
      301 gacggcgacg atgtcttcga cggggatcac gaacagctgg ttgtcggact cgaaatcgac
      361 ggggatggcg ttcttcgggt gaaacaggat cttgtcgtac tggcggagcg gcaggtcctc
      421 gtcgttctcg atctgggccg aaatggcgac gatccggccg gtgatcgtgg gaatctcggc
      481 cgcgtcgggc agggcgatgc cccccttggt gtgccgcttg gcgtcgtcct tgcggaccag
      541 cacgcgggcc ccgatcggct cgacgtattc gaagactttc tgacgggtct tggacatggg
      601 aaactcaggt ggtagcccgc gactccgtcg cgggttcccg ccacggagtg gcgggctacg
      661 gcggccgccg gagtggcggg ctacggcagc cgccgtcaca acattactcg gcacctggat
      721 ttgcggcaag ccgcaggccg ggagtcgggg gtcgggagtc aggagtcgga gaaagtcagc
      781 ctccgactcc cgatccccga ttcccgactc ccgactcctg acgtagcgtc actcccgcaa
      841 aaccgacata ataggaacag cttaccgaaa ctgccgcggc tgttccggcc ggcggctgcc
      901 gccgattggg gatttaccat gcgcaagcta ctactcacgc tggccgcgat ttcgctgggc
      961 ttggtcgccg gaccggccgc cacgctggcc cagaacgccg gccaggaaga cctggacaag
     1021 gccaccgagc tgcaaatcac ggcggaaacg ctgggcgaac tcgagcaggt catcaagctg
     1081 gccgagaccg ccctggataa gggcctcgac aagggccaga ccgagttcgc caagaagctc
     1141 ctgggggcga cgctgttcca gcacgccgaa cgctccgcca ggtcggtctt cgagcagatt
     1201 ccgcccagcc gcaactggcg gtcgatccgc gaccagggga tgaagaacct ggccaaggcg
     1261 aagaagttcg atccgacgct gccggacgtt tacctgctgg aggccaagtt ccaggccctg
     1321 cccggcggcg agacgcggga cgcgatcgcc gcgctcgatg aggcgatcaa gctcctcaag
     1381 ccaaaggatc agcccaagca gctggccgat gcgtacgtgc tgcgggccca gttcctcgag
     1441 gacgacgagc gcaagctggc cgactacgac gaggccctca aggtgcagcc caagcatgtg
     1501 gccgcgcggc aggcgcgggc cctgcactat ctgaccaagg gagagaacga aaaggcgatc
     1561 gccgatttcc agaagctgct ggagcaggac gcggacaatc cgggcgtgat cgccgaactg
     1621 gcccgggccc tgatcctgaa caagaagttc gacgaggcga tgaagtactg cgaggaggtg
     1681 atcaagctcg ccccgaaggc gaccgagggc tacaagctgc gggcccagct ccgctggatc
     1741 aaggaggaca ccaagggagc cctggaagac ctgggcgagg ccctgagcat caaccccaac
     1801 gacatctctg ggctgctcat gcggtgccgg ctgtacgctt cggagaagaa ggacgccctg
     1861 gccaaggccg acctcgaccg cgtgttgcgg ctgcggcccg acctgccgca ggcgatcgag
     1921 ctgcggagca cgatggcggc cctgaagcag aactacgccg acgctatcgc cgacatccag
     1981 acgctgctgc agggggaccc gacgaacccc gagctgcggc tgcagctggc gtcgtactat
     2041 gtgggcgaca agcggccgcg gaaggcgatc gagctgatca ccagcgtcat cgacggcctg
     2101 gccgacgaca aggacgccgg tgcgaaggag aacaaggccg acgccctgcg gcagcgcggc
     2161 gacgccctgc tcagcgtcgg caagcacgcc gacgcggtga aggattacga agaggccctc
     2221 aagctcgacc cgaacgatac gggcgtgctc aacaacctgg cctgggtgct ggccaccagc
     2281 cccgacgacc cggtccgcaa cgccaagcgt tcgctggagt gcagcctgaa ggcctgcgag
     2341 ctgaccaagt acgagaagcc gcacatcctc agcacgctcg ccgccggcta cgccgagcag
     2401 ggcgactggg agacggccat caagtggtcg ggcaaggcgg tcgagctggg cgtcgaagag
     2461 gtaatggtgg acgaccagct gaagaaggag ctgcagagct acaaggacaa gaagccctgg
     2521 cgcgagaagc aggaagtcga ggagaacacg aagccgctgg ggaagaagtc ggacctcgag
     2581 acgtaatgga tcgctccgcc gctctcgcgc tcgttcacga atacactccc tcgctctcgc
     2641 tgcggcgtca catgctggcg gtcgaggcgg cgctgcgggc ctatgcccgc aagttcggcg
     2701 aggacgaaga gaagtggggc ctggtcgggc tcttgcacga cttcgactac gagcgctggc
     2761 ccaatccgcc cgatcatccg ctgcaggggg cggcgatcct ggccgagcgc ggctatccgg
     2821 ccgacgtgat ctacgcgatc aagtcgcacg ccgattacct gcccgattgc ccgcgcgtca
     2881 gccggctcga caagacgctc tacgcctgcg acgagctggc cggcttcatc accgcctgcg
     2941 cgatggtccg ccccgagcgg ctggcgggca tgcaggcctc gtcggtgaag aagaagctga
     3001 aggccaaggg ctttgccgcc agcatcaatc gcgacgacat cgcccgtggc gccgccgacc
     3061 tcggcgtcga tctcgaccag cacatccagt tcgtcatcga cgcgatgcag cccatcgccg
     3121 gcgagctggg actggccgcg acgccagcag cgtagggtgg gtccagcgag tccgcgagcg
     3181 ccgacccacg cggcatcggc gtggtctccg cgtgggtctg cggccgcggc ggccttgacc
     3241 caccctacgc ctacgcaacc gcctgggcca cgacgatcac cggccgccgg ttgcggcggc
     3301 gggcccacca cacgagcggc gcgaggacga cgcacagcgc cagcgagatg gccgccagcc
     3361 acggcagtaa tccgacgatc accagcacgc acgcttcgcc gcacttgcgc agctcgttgg
     3421 ccgagtccca gaaggtctgg ctgatcttgc cggcaaacgt cgtggccagc ggcggccggt
     3481 aatccagccg ctcgtaggcc gtgatatcga cggtggacag cgcgacgcga tctcgcagct
     3541 gccgcagccg ggcctgcatc cgctcgatct cttcgcggac ccgcgccaat tcggcttcca
     3601 gggccagcac gtccttgatg tcgtcgctcc gccgtgcgac cagctccagc aggcgggcct
     3661 ccagcgcctg cttgttcttc gctcgtgctt ccagatcgac gtattcctcg gtgacgtcgt
     3721 cggtgtgcgt ttcgcgctgc tcggcgattc cgagcaggcc tgcctgttcg acgaagtggg
     3781 caaaccgcgg cacggggacg cggatggtcc actgcccgcc gcgccgcgcg cccgaggaac
     3841 ggtcctcgcg aaactgggcg acgtacgcgc cgtcggcgcc gatcagctcg ttcagtctcc
     3901 gctcggcctc gccgaagctt tcgacgcgga ccaccagcag cgcccggcgg atgatctgcc
     3961 gcccggccgc ggccgccggc tcggccggtt gcaccgccgg cgctgccgct tctgccctgg
     4021 gcggcgcttc tgccgcgcat ccggccaaac ccacgagcaa caagagtccc agcgcggagt
     4081 cgttcatagc gccgcctccg gtgaacaaag cgttgtggta ctgcttgatc gagccgagcg
     4141 ggccgcggga tattcccgcc gcgcaagaat tctggccgcg ctacttcgcg gcaatcactc
     4201 gccgccgtag ggtgggacca gcgagcggag cgagcaccgg cccaccgcgc gtgactaccc
     4261 gaccgcgtag cagcgtaggg tgggtccagc gagtccgcga gcgcagaccc acgcggcatt
     4321 ggcgtcgtct ccgcgtgggt ctgcggccgc agcggccttg acccacccta cacctctcgg
     4381 gcagccgtag ggtgggacca gcgagcggag cgagcgccgg cccaccgcgc gtgaccgcac
     4441 caaattggtg ggccgtcggc cgccgaccac cgcttcgcgg cggtgcccgc cttgtcccac
     4501 cctacgcgct acccgacggc gtaccggtcc tcgggcaggc ggtagaactg gcagatgatc
     4561 tcccaggcct cgtgcggcgt ctcggcgtag ctgaccagcg agaggtgatc gtcgcggatc
     4621 gtgccttcgt cggccaggaa ccgcaggttg aggacgccgt cccagaactc gcggccgtag
     4681 aggatgaccg ggatcggctg catgcggttg acctgccgca gcgtgagcac ctcgaacaat
     4741 tcgtcgagcg tgccgaagcc gccgggaaaa acgatcaagc ccttggcccg cagcaggaag
     4801 tgcatcttgc ggatcgcgaa atagcggaac tgaaagcaga ggtcgggcgt gatgtacggg
     4861 ttgggcagct gctcctcggg cagcgtgatg ttcaggccga tcgacttggc ccccacgtcg
     4921 gccgccccgc ggttggccgc ctccatgatc cccggcccgc cgccggtgac gatcgcaaag
     4981 tcgcactgcc cgtgcacctg gcaggccgcc gagaccaggc gggcgaactc gcgggcctcg
     5041 tcgtagtagt gggccttggc cagcacccgc tccgcccggc tgaccgcccg ctgggccagc
     5101 gggtccgccg gatcggcggc cagcgctgtc ttcgcctcgg ccagccgccg ctcggcctcg
     5161 ggccgctcca cgatctgcgt gccgccgaac acgaccagcg tcgaggtgac ccgctgctcc
     5221 tcgaaaatca tgtcgggctt gagcagctcg agctgcagtc gcaccggccg cagctcgggc
     5281 cgcttgagga acttgccgtc gtcctcggcc agcaggtagt tgggaaagac gctttgcggc
     5341 tgcggcgaag aggtctcgtt tagcacggcg ggggcggtgg gtcggtggtc ggtaaatcgg
     5401 tggtcggtgg tcggtcggtc ggtgagtcgg tggcgggtgc gaggtcggcc aggaacgact
     5461 gcaccgtcca cagcaggcca gctgccacaa gatacagggc cagcgccgga acggaccaca
     5521 gcaatccgcg ctgccagccg agcacgatgc acagcgacgc ggcccacgac acgggcgcaa
     5581 acggcggcca ctcgccggtc ctgaaccacc gccacaggct gccggccagc ccgccgatca
     5641 cgcccgcggc cagtcccgcc agcccgtcga tcagcccggc ctgccagtcc ggcatcttgc
     5701 cgtagcgcca cgccgcgatg ctgcgaatct ccggccagat gaccggcaac agcagcgcga
     5761 ggccgatcgc cggccagaac agccgcgagg gcgtgcgaaa gctgtcgccc aggatcagtg
     5821 cgccgcccac gagcgtcgcc agcaggacga cgtgcaccgc gtagcggctc cagtagggcc
     5881 agaggtcgta cgcgccgctc ggcagcgggc ggaggggaac gccaggccag acgccgatga
     5941 aatgggtgcg ctcccaaaac gcgacggcca ggaacagcgt cgccacgaag agctcgaccc
     6001 agaaatagcg cggcgagatc ggcgcgcggc aatcgcggca gcggccgcgc aagagcagcc
     6061 agctcaggat cgggatgttg tcccacaggc ggatcgcgtg gccgcagctg gggcagcacg
     6121 agcccggatg ggccaggctc ctgccccgcg gcaggcgata gaccacgacg ttgaggaagc
     6181 tgccgacgca ggccccgacg cagaacagcc acacgtgcaa gaggaccgcc gccgtccact
     6241 ccatcaccca cagtggtacc cgcggctcgc ggatcggcgg cgcggcaaac aggacggcgg
     6301 gccagacgtc gaattccatg tcaaccgcaa gcgtacgcta accaggagtc caactcgtgc
     6361 ccaagctccg cttgggcacg caagttccga agctctgctt catccgcacg caggaagcgg
     6421 agcttccgta cgtgggttcc caagcagagc ttgggaacca ggagtgccag gcagtgctag
     6481 catactccgc ggcgccggca agcgctgttg gtgatctctg ggcccgcgct atacaaccaa
     6541 ggccggcctc gcggaaggcc gttcaccaaa ggatgctctc atgcgatgga ttgcttcgct
     6601 cgccgcgttt gcgctcgcgg ctctgctggc ccagcccgcc gccgcccagc tcgtcgccac
     6661 cgccgccgcg ccccgctcgg ccgaggccga ccagatccgc ggctggtacc gcgactacct
     6721 gggccgcgag gtcggcccgg agctgaccgc ctgggtcgaa ttgctcaagg gcggcatgtc
     6781 ggcgatcgac gtgcaggcga cgatcctcgg ctccgacgaa ttctattatt ccaagggccg
     6841 cgatccggag tcctgggtcc gtgaaacgct gcaggcggtg acctgggccg acccgaccta
     6901 cgccgagctc cgccgctgga acgatcggct gagccgattg cacggcgacc gctttgccct
     6961 gtcccgcgag attgtcctct cgacgtcgca gccgcaaacc cagacgccgg cgggccaggt
     7021 cggtgacctg gccgcgcggc tggccagcgc ggcccggctg gccgtcgata cgatcgactt
     7081 cgaaatcggc ggcacgacgc aggggcagca ggccaacctg cgggcccagg cgctgcttag
     7141 cgcggccagc cagctcaacc aggtcgtcac gctgcggagc taccgcccgg atgacgccct
     7201 gcgggcgctg gacagcgccg accgctcgta tcaagcgctg cagcagacgc tgaacagcct
     7261 gccgggcagt gcgccctcgg ccacgggaat cgtccgccgc atcggcacga tgctgaccga
     7321 cgcccgcggg gcgatccgcc cgccggtagt cggtccgacg tatcccacga cgccgacgat
     7381 tcccggcggc tacggtttcg atacgcggca gctcaccgcc caggtcgatg cgatctcccg
     7441 cggcgtcgaa tcgctgacgc agctgctgac cagccaggcg tatcagaact acgcctacag
     7501 cgccctcctc cgcgacctgg acacgtttgc ctcgcgggtc gatgccttcg agcaggcgct
     7561 ggtccgcggc acgtcgcgcg accgcctgag ctgggagctg gagagccttc gcgaacaggg
     7621 cggccgcatc cgcacgcagc tgctcgccgg ccggccgccc tatttcgccc ggctgtactg
     7681 gcagagcgtc gaaagcggcc tcgatcaatt gcgcgacacg ctgggcccgg ccagcaccag
     7741 cggcagcccg gccggcagcg gggtggccgt gctgcggccg ccgctgtatg acaacctcgt
     7801 gacgctgctc gaccaggcgg cccagcacct ggaggtcttc ctggcgggca ccaatccgct
     7861 ggtctatggc attcccgacg tgccccgcgt gcagcgcgac gcccggatcc tgaagaaccg
     7921 catcctggag ctccgccagc aagccgccaa cggcgatccg gccccggtgc tcaagcagac
     7981 gctgcagacg atggtcggcg aatacaaggt cgtctacgac cgctggaacc aggtcgtcgc
     8041 cggcaaccgg ctgatcaatc cggcccgcct gtcgccggcc ggcgagacgc tcaaccgcgt
     8101 cgagcagctg atcaacgacg ccctggcggc cggcgacctg cccgcggcgg gcccgacccg
     8161 cgccggccag ctgctggcgg cgctgcagaa cgacgtgaac agcggccgcc gttcgctggt
     8221 ggcgtttggc ggctaccgcg agcagcaatc gatcgacacg tacttcgggc agctcagcga
     8281 ctacgtgcag tcgatcaacc agtcgcagac gcggacgacg aacgtcgatg cccgccggct
     8341 ggccgtcgcc atgcaagggg tcgtcggccg gctgcaaaac gacgccgacg ccctgaacgc
     8401 gcgggtcgat tccgccggca ccgtgcagca gcgccagcaa gccaccgcgc tggtccagct
     8461 cgccgccagc atcggccggc tggtcgatga gatcgaggcg gcgctgtact aactcaaagc
     8521 cgcgaccagg cgtagggtgg gtcaagcgag ccgctcgcgc agacccacgc ggtgaatacc
     8581 tcattcagaa attgattccc gaaaacgaat cgctcgccgc gtgggtcggc gctcgcctcg
     8641 ggcgagcttg acccacccta cgcgaaaaac gaaaggactc gccatgcgtt gcctgctcgt
     8701 cattacattg ctctgcacgc tcgccctgcc ggcggcggcg cagacgaaac ccgcgattgt
     8761 cgcccgcaag agcagcgaac aatcggccgc gaactttcag gccgtgatgc agcaggcgct
     8821 cggcgctttg gcccgcgccg gcagctacgc cctcgacgtc cagagcgagt ggggcgcggt
     8881 cgatgatccg aatggcccgc agggcggcag ccgctatcgg ctggtggcgg ccggcggcaa
     8941 gtaccgcgtc gaggtccgct cgccggcggc ccaggcgccg gagctgctct gcgtcaacga
     9001 cggccggcag gtgacgacgc tgctgccggc ccgcgggctc tattcgcagc acgcggccgg
     9061 ctcgccgcag gccagcctgg aggccaacac gatgctgagc ctcagcctgc agggctcggc
     9121 gatcgacatc ctgctgcagc ccgacgtcgc cggccacgtg cggacgcagg ccagcgacct
     9181 gaaggatctc ggcgcggggc agctcggcag cgtcaaggcg caccacttcg agctggtctg
     9241 ggccggcgcg aaggtgcagc tctggttcgc cgccgaaggg gagccgctct tgctccagtt
     9301 ccgccgcacg acctgcgtgc cgaccgggac gagcgacttc tacgagcagg agtgcacggc
     9361 gaagttccag tggcagctgg gcgtcaagcc cgcggagggg acttttgccc tggccctgcc
     9421 caaggacgcc cgccgcgtca acgaaatcta cgacgccctt tccggcgaag aatcggccag
     9481 ccgcatcggc aagccgctgc ccaagctgca gctgggcaag ctcgacggca gcgagatcgc
     9541 cctggccgcc gccgagggca agaaggcgac cgtgctgatc ttctgggcga cctggtgcgc
     9601 atcgagcacc gaggatttag ccacggtgtc gcagttcgtc gccgccaaca aggacaaggg
     9661 gattgccttt tacgcgatca acgtcggcga gcagccgggc gaagtccgcc gcttcacggc
     9721 caagagcccg ctggtgagca cggtgctgct cgatccgcgg ggcaaggcca gctcggccct
     9781 ccgcgtcacc gagctgccgg cggttgccat cctcgcgccg gacaacacgg tccgcgccat
     9841 cctgcacggc accgccaagg agctgcagag cgaattgacg gcccagctcg aatcgctgct
     9901 ctcggccagc accgcccgtt ctccgggcga aaccacgcgg ccgaagtagc gtcctaggtc
     9961 tgatcctaat cctaatcgta atcctaatcc tgcggcctcg actgcggctg cgccgccgag
    10021 agaacgatta agatcaggat taggattaag attaggaagg gagctaagcc cgacgctcac
    10081 tgcgtttgcg gacgcgggcc agctgcgagc gggcggcggt cgtcacgctg tcgggcacgt
    10141 tgggcaaggc cagcacgcgg gacaggtctt ccaacgcccg ctggtcctct ttgagggaag
    10201 agtgcgccag ggcccggttg aagagcgcct tggcgcggac ttcgcggtcg gtcgtggtcg
    10261 aggcgaccag cgagttgtaa atctgcatcg cccgctcggg ccgattcgcc ttggcatgct
    10321 ccatcgcctg ccgcagccgc tcctcgtccc gtccgccgcg cgaaaacatc cctgtcaggc
    10381 gactgataag attcatgcgg actccccccc aagttgcaat acgctactcg actggagttg
    10441 gcgacgcaat cggatttaat cttcgctgat gctcgctagc gaagcgggag ggcggcgcct
    10501 acgacatatc gtcgcagtcg tcgctcgctg gccccgcgcg tcgagacggt tgattgccga
    10561 tcgctagttt ccgctcaact cgccccgcag ccagcgggcg agcagctcga tctgctcggg
    10621 cgacaagagc ggttgcttgg ggccgcgggg gtccttgcca aagctgggca tgcggtcgtt
    10681 ggtggcccgg tagaagtcgt cgtgcgtcgg atcgctaatg aatcgcacga gccagtcctt
    10741 ggacgcccag ccggtgagga cgggggcgct gccgacgtcg ccggcatcgc ggaacttgtg
    10801 gcactcgacg caggcctggt tctcgaaggc ctcgcccatc gccttgcggc cccgctcgag
    10861 catgccgtca tcggcggcct tcttgtcctc gtcggcctgg gagggcagag cagcttccgc
    10921 ggaaagcgcg gcgacgatcg cggcgacttt ggccttggcg gcgtcgttgg gctcggccag
    10981 atcgctctcg acgaagccca ccatgtcgcc tttggcgtga cgcgtctgac caaaatatct
    11041 gtcgctctgg atcagctcca ggtcgagcat tccggcgagc caggcccgcg agccgaagcc
    11101 aaacaaattc ggcgcgctcg gccacgcggc ggcaatcccg cggccgtgct cgtaggtatg
    11161 gctgtggcag gcggcgcagt tggccgcgaa cagccgctcg gcctgctggg cctcgctcgg
    11221 cttcgccgcc tgcggcgctc ccacgacgct ggtccacggg ctggcggtcg tgcgggccag
    11281 ctgcactgcc ttctgggcca cgtcctccgg atgcggcagc cgcggggcgg catcgcccgc
    11341 gcggtaatag tcgccgcgga gccaatcgac gatcagcgac agctcgcgga cgctcagctg
    11401 gttcttctcg ggctgggcca ggttggccgc gaagctgggc atcccgtcgt tctcttgccg
    11461 gtagaaccgc tcgtgggccg ggtcgctggc gagccccagc atccactcgt acgagccgta
    11521 gccggtcaag tcgggcgcga ggccgagctg gccgctggcg ccgaacttgt ggcagccctt
    11581 ggtgcagttc agctcgatca gcccgacgcc ccgctcgatc agcgttttat ccttgccgtc
    11641 cgcggctgcc tggcccggca gcttcgcctg ggccgagagg gccgtgacga tcgcgtccag
    11701 gtcgtcgtcg gacttcgcgg gatcgtcctt cagcagcttc gcagagtgct ccttgaccca
    11761 ggcggccatc cggccggtct tgtggccggt gttgccgaaa tagggcgcgt cgatcggccg
    11821 cttgtggttg tccgggcggt ccttgtcggc ggcgatcttt gggtccggcg agggctgggg
    11881 cgggccgatt tcgaatcgga cgagcaggtc gtggttgagc agccccttga tccactcgcg
    11941 gctggcgaag ccgtacaggt tcggcgcgct attggcggca tccccgtagg tgacttgctc
    12001 gtcggcgctg cggacgacct tgccgccctc gccgacgacg ggcgactgga acaggccggc
    12061 cacttgcggc ggccgctggc ccttgggatc ggcatagtcg tggcagcccg cgcagtgctg
    12121 gccaaacagc cggggccctc tggtcagcgg gtcgttgcgg agcaggtaga ccgcgccctg
    12181 cttgggaatc agacgcacgt cggagagctt gccatccggc agcatttcgc ggcggttgat
    12241 gagctcgatc gagcggcggg cctggatttc ggcgtcggcc cgggcggcga ggaactcctt
    12301 cgacgcggtc agtttcttgt tgtcatggag tcccagccgc ggcgccacct gggcgtagta
    12361 gtcgtcgaac agcgccgcgc ccgtgagccc gacggcggcg agcagcacca gcaccgtgaa
    12421 ggcctggttg aagcggtggc ccaggttcga ccagccgatg aacggcatca gggcgaagat
    12481 cgtcatcacg acgccgggaa tgacgatcgc gccgatgatc tcggacgagc cggggaagta
    12541 cttcaggagc tggaacagga acaggtagta ccactcgggc cgggcggcgg aataggcttc
    12601 cgcgggatcg gccggcgcgc cgagctcggc ccccaggtgc gccggatcga acttgccgga
    12661 cgccagcgcg ttcagatccc agtggaagac gcagagcagc acgatcgcca gcagcaccag
    12721 gcagccggcg gcgtccagca ggacctgctt gggccagaag gtctcgtccg cgcggccggg
    12781 cgtgatggtg gccatgatgc cgtggcggcg gaagagggcg atgtggaccg tcagcgcggc
    12841 gatgagcagc accggcagca cgccggcgtg catggcgaag aaccgcgtga gcgtgtggtg
    12901 gccgtattcg ttgccgccga cggccagctg ctgcacctgc ttgccgacgc cgggcacgag
    12961 cgtggccagg ttcgtcgcca cgttcgtcgc ccagtagccc ttctggtccc acggcagcag
    13021 atagcccgtc aggcccagtc cgagcacgag ctgcaacagc accagcccca gccagtggtt
    13081 gacttcgcgc ggggcgcggt aagccccgtc ccacacgacc tgcaacaggt gcagggccat
    13141 caccacgacc atcgcctggg ccatgaagtg gtggatgccg cggaccagcc agccgccggc
    13201 catttcgtgc tggatgaaat agacgctttc gtaggcggtc tggctgctgg ggctgtaggc
    13261 catccacaga aagatgccgg tgacggactg cgtgacgaac gcgaacacga gcatgctgcc
    13321 ggtgatgtac cgccagcggg ccccgctggg aatccgctcg tagagcgcct cgttcacgat
    13381 ccggcgatag ccggtgcggc tgtcgagcca gcctaagagt ctgttgagca tggtgttgtt
    13441 ggttgtcagt agtcagttgc cagtggtcag ttgttgaaag cgccttccca ctgacaactg
    13501 actactgact actgactact gactactgac cactgaccat cacggcttct cctcgcggtc
    13561 tggctttccc gggtagtagt tcacgaactg cacccagact tcgccttcct ggagcttcgc
    13621 ctcatcgact ttcagggcgt ccatgtcgcg ggggctgggg gaggggagaa tccgctggcc
    13681 ggtgagctgg aacgagctgg tgtggcaggg gcactgaaac accttgcggt cggcggcata
    13741 gcccacgaag cagccggcgt gcgggcagat cgcattgaag cactccacgc cctgggccga
    13801 cttccgcagg tagaccgcgc cgaccggctg attgggctcg cggttccagg cgtcggtcag
    13861 gtcggcaatg acggggactt gcacgggcgt gccatcggcg ggcagggccg cggccgaggc
    13921 gacgcggcgg aagggccgct ggtcctcgcc gccctggctg ttctgtttcc gcttgagaat
    13981 cggatcgaga aagaccagca ggcccgcgcc gaacggaaac agcccgacga ccgccgcaca
    14041 gatggccgcc agacaagcga cgagcacgtt gcggcgcggc gctgcggccg gcggcggcgg
    14101 gggtgcggcg tggccggcgg catggggatt gagcgatttg gccatgcggt tactccgcga
    14161 tcaatcgcgg cgggcgagcg cgtttgcgag gcgggatcga ttccggcggg accgcgatta
    14221 tcgcagcgca cgaagcagag cgtcaagaag cttcgtttaa cccgtagccg gaggcgcagg
    14281 ctctttcggc atagccgccg tcgcctccgg gttaaacgaa ggaggttacc acgtgccttc
    14341 cgagtacagc accttgccgt ccaccgtcag gcgaaacgcg ctcaggcgga ggattccgcg
    14401 ggcttcgatg caggcgcgct ttctcatcgt gctgccgaac aggtcgaagt ccagccgggg
    14461 ctgaagccaa atccaatcgc tgagcttgcg ccagtcgccg tggatgcgga cagccagcgc
    14521 accgttgacc agcaccgact cgtagccgat cccgcggccg ttgtaatcga cgatgcacgg
    14581 gcagggcccg gagaccgcga agcgccgccg catccagccg cgcgacaata gctgcgccga
    14641 gaactcgacg ggcgagaatt ccggctcgcc gcgcgattga gccagcggcg aggcgtaggg
    14701 attgacggcg ctcgatgcga cgttcacagc ggaggcgtcg aatgcgcggt ggggggagcg
    14761 cagctggaat cgccgcagct gggagcgacg acggccccgc cgcagccgcc ggaagccggg
    14821 gcgacggtgg gaaatacgta ccgcgtggag cggtcgctgc tgttagtcac caggccctgg
    14881 aaccagtcgc cgttgccgaa gccgtacgag ctcgcgccgt aggccggccg ggtgaacgta
    14941 tagatggcgg tggtgagggc cagcaggatc gaggccgcgg ccaaaagcga tgcgccggtc
    15001 agccgcacgg cagcggtcat gctctcgagg acggccagca cggccttggc ggcggcgggc
    15061 gaggcgactt cgcggagctt cgcgccggcc agcagctttt gccccgcaat cgcggtggcc
    15121 tcggccagcg ccgcgggcac ggcggccagc gtctgcttgc agaacaccgc ggcgatcagg
    15181 gcggctgtca gcgccaggcc gcggcgcatc aggcgcttcc gcaacagctc gcggccgcgc
    15241 gacaagcgcc gcgacatgga gccctcgggc cagccgagct gctcggccgc ctgggcgttg
    15301 gtcttgcctt ccaggtagca gagcaccagg ggcagcgcgt atttctcggg cagctggccg
    15361 atctcctcgt cgaggatcga ccgcagctcg tcgtatttca actcgccggg gtcgctgggc
    15421 agcggacgtg gggcgcggct ctcgcagctt ttccgcaggg ccgcctgcag cttgaccttc
    15481 cgcgccgtgc ggttggccac gccgtacagc cagctgctga gcttgcccgg ctgccgcagc
    15541 gaggcgccct ttcgcacgag gacgaggaac gtcgcctgaa acgcatcctc ggcgtcgttc
    15601 gagtcgctca ggatccgccg gcagacgcca tagacgacgg ggccgtgccg cttgacgatg
    15661 gcggcgaacg cgccgtcctc gtgctgcttg aaaaagcggg tcagcagctg ctcgtcgggc
    15721 agcagcaata aatccgcgac ggccgcttgg gcggcgggat cgagcgcagg cgcagccgtc
    15781 agacccagtg ccatggcgaa tcctccagca ggcttatcca ttggcttact atcggcggga
    15841 gtggaatgca agggtcggga gtggtgagtg ttcaaatcgg atgctaaccc caaggcgtca
    15901 gcgagggact tctttaa
//
LOCUS       DASRJB010000560         9965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1568902, whole genome shotgun sequence.
ACCESSION   DASRJB010000560 DASRJB010000000
VERSION     DASRJB010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568902"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFB80_14615"
     CDS             <1..608
                     /locus_tag="VFB80_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN35058.1"
                     /translation="LLIELDGLEAGLDEQRDRVVEVCMQNRAREVRRADDPKERLKLW
                     KSRKQAFGAIGRLSPSYCTQDGVVPRTRLPHILRKIIEIGRRYDIRIVNVFHAGDGNI
                     HPILLFDERNAEQVRRVLAASNEILDACLACGGSVTGEHGIGVEKIAFMNKMFTPSDL
                     AAMKNLRDAFNPRGNLSPYKMLPTAAGCGMEQKHPGRRAAL"
     gene            892..2142
                     /locus_tag="VFB80_14620"
     CDS             892..2142
                     /locus_tag="VFB80_14620"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN35059.1"
                     /translation="MSDAALPIKDTLSPASHDEVAAAVRAAFDSITPLYPIGGGTALG
                     FGLPAKAPGRGLSLAKLNRVVDYPARDMTVTVEAGITMHAVGELLAKERQRLPIDVPQ
                     AERATIGGVIATNWNGPRRFGEGTVRDYVIGISAVDGRGQPFKGGGRVVKNVAGYDFC
                     KLLTGSLGTLGVITQVTLRLKPIPEQSALVACAVADSAVAEKLVAGLVNSKTTPTAIE
                     LLAGPAWQNDPALAGLGALVKSSLYLVVGLEGTGPEVEFMTRQLASEWRAAGVDSLVV
                     GEAAALWRRLVEFPAEGESPLVLKASVAPSGVAPVIEAARRLDPECSLQAHAGSGIVL
                     IKLSKFPEQGLSRALVGNLQPAAAAHLGYLIVLSNPSGAEMTHQCVWGATDVPLWLMT
                     EVKRKFDPQDILNRGRFVYLGRSQ"
     gene            2382..2597
                     /locus_tag="VFB80_14625"
     CDS             2382..2597
                     /locus_tag="VFB80_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35060.1"
                     /translation="MQPAETMTRLNLLTDEGPIAVLFTPALESTHYSELFRLSKEFAS
                     EADMRAIVAAAAERWGRDVVISSYSSN"
     gene            3293..4696
                     /locus_tag="VFB80_14630"
     CDS             3293..4696
                     /locus_tag="VFB80_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZN35061.1"
                     /translation="MTSPAADAAPQASAPALAQPLQESTALQLSQIGPAPQANGAPGA
                     AIDYELFLDCVHCGLCTASCPTYVETGNENDSPRGRIYLMRAVRDGRLTLNHEVRRHL
                     ELCLDCRACETACPSGVQYGKLIEPFRVSMEGHAGTNSAKANDWFHKYILFGLFPYPE
                     KLRQSLRPARVAQSLGLDKLLIATGLWRLLPPRLGRLVTMLPPLEKPLPALPEFLAAK
                     GKRRARVALFTGCVGDVMFRHTNWATARVLQENGCDVVVPQAQVCCGAIHFHAGSSPP
                     AREMADKNVAAFDAAGVDAVIVNVAGCGSMLKDYGHHWHDEHSAAREQFAHKVRDCSE
                     FLDQLGLIDPPGEIRLTATYHDACHLAHAQKVREQPRNLLRKIPGLKLIDLPESDLCC
                     GAAGTYNLTEPEMAGRLGKRKLDNIRSTGAQVVITANAGCLLQIAREARLRGESLKIM
                     HPMELLDLSYQRQGVRF"
     gene            complement(4693..7002)
                     /locus_tag="VFB80_14635"
     CDS             complement(4693..7002)
                     /locus_tag="VFB80_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN35062.1"
                     /translation="MLAVSAVEPTWLEMIGTWVWMILGVAGGLTFVIFVHELGHFLVA
                     KACGVKCEKFYVGFDFFEIPIPFTKWKLPRSLFKFQWGETEYGLGSLPLGGYVKMLGQ
                     DDDPRNAEAEAARIRTAAPAASDARVEAIASGDAAEGLVAGQSVEKLTNEALAAAHVH
                     DKDKPADPPVPAQTTAGRTILLDPRSFPAKSVLARMAIISAGVIMNLIFAVIMATVAF
                     RLGVKEMPAVIGATSPGGAAWVEGLQPGSRLIQIGERGRRNEFLSFTDFRQTAMLNNG
                     RPVKLLVREPDGKEDWHELTPTTKPGGKSPMIGIAPAEDLELNIWPGPASYLNPKSSA
                     QLLERDKVVEAAGQKVASGAELGAVLAQQPLGQIAIKVERRERQPSGELVKDSKRQPQ
                     FVDVTLEPRPMRELGLTMKMGPIVAVRKNSPAQLAGLLPDDTILEIDGEPPGDPLSLS
                     QRLTPKPGSTEPLKITVQRKDRQGQPVTKTLEVTPEPPLQSALHPPAMNPTSIESIGV
                     AFAVMNEVNSVDSAGPAGAAGLRAGDFVTKLEFVPGGRPEDVKKIKKIAGSFRFDALE
                     FAETKDSWIDAVVGLQMVPADTKIKLTWLRGKQTMSAVMSPRDSATFFDDRRGLKTYA
                     LTQTQIGTNWGDAFQAGAQETVERVEEVLVILYSLVTGRLPATNLSGPPGILMAAAGF
                     ASEGFSSLLMFLVILSANLAVLNFLPIPVLDGGHMLFLTYEGVRGKPASGELQYRLTL
                     LGLLCLLALMVFASAMDIGRFAELIRNWF"
     gene            complement(7002..8222)
                     /gene="dxr"
                     /locus_tag="VFB80_14640"
     CDS             complement(7002..8222)
                     /gene="dxr"
                     /locus_tag="VFB80_14640"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZN35063.1"
                     /translation="MDHPPRRIALLGSTGSIGQSTLEVVAASRGRLEVVALAARQRLA
                     DLCKQARQLRPRFVVATDAEAARGFDWSQLPRETEPLIGPEGLSRIVRLPEVDIVLSA
                     IVGSAGLTGTWEALDAGKPVALANKETLVMAGPQVMRLSAQRGAKILPVDSEHSAVFQ
                     LLEGQSRDQWSRCTADDPPTPPRPLVAALKRIILTASGGPFRCWSREQMETATVADAL
                     AHPTWRMGDKITIDSATMMNKALEIIEARWLFDLPPEQIEVVVHPQSVVHSLVEYVDG
                     SVLAQLSPPDMKLPIQYALTWPERQPSPARRLDWTEALRLEFEPPDEDRFPALKLGRE
                     VAAAGGTAGAVLNAANEAAVASFLAGEMPFMEIVPACRAVLESHHFDAEPSLDELLVL
                     DGWARQEMQRWIVA"
     gene            8583..>9965
                     /locus_tag="VFB80_14645"
     CDS             8583..>9965
                     /locus_tag="VFB80_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN35064.1"
                     /translation="MSQDNGSGRQKPEGNGSSGSNLLLWAGLIVATVLLVVLWVTPWL
                     TKELRPEDLQALIKAGAKDTSGNPVNTVEYVDVRAPNNKYYRYSKLQKVVIRERSITG
                     QVDLVELVQQGDGEAKKLVPNGKTRSEAQAFRTDIDPEGEYRAQFPKLLRENNFTDWK
                     FDTTQNTWEQHTPWILFTLLAIFLIYFMLRRLIGAGSPMSFGRSRGKLYAQEDLGVTF
                     NDVAGIDEAVEEVREVVDFLRSPDKYQRLGGRIPKGVLLVGPPGTGKTLLAKAIAGEA
                     GVPFFSLSGSDFVEMFVGVGAARVRDMFTQAEAKAPCIIFIDELDALGKSRGTSIVGG
                     HDEREQTLNALLVEMDGFGSNSGVIVMAATNRPETLDPALLRAGRFDRQVLVDRPDIR
                     GREAILKVHVKNVKLDPTVELKEVAAITPGFVGADLANLVNEAALLAARGEKNAVGMK
                     EFNEGVERVTA"
BASE COUNT         1801 a         3307 c         3171 g         1686 t
ORIGIN      
        1 tgctcttgat cgaactcgac ggcctcgaag caggccttga tgagcagcgc gaccgcgtcg
       61 ttgaagtctg catgcagaat cgggcccgcg aagtccgccg ggccgacgac cccaaggagc
      121 ggctgaagct gtggaaaagc cgcaagcagg cgttcggcgc catcggccgg ctcagtccca
      181 gctactgcac gcaagacggc gtggtgccac ggacacggct gccgcacatc ctgcggaaga
      241 tcatcgagat cggccgccgc tatgacatcc ggatcgtcaa cgtctttcac gccggcgacg
      301 gcaacatcca cccgattttg ctattcgacg agcggaacgc cgagcaagtg cggcgcgtgc
      361 tggccgccag caacgagatc ctggacgcct gcctggcctg cggcggcagc gtcaccggcg
      421 agcacggcat cggcgtcgag aagatcgcct tcatgaacaa gatgtttacg cccagcgacc
      481 tggccgccat gaagaacctc cgcgacgcct tcaatccccg cgggaacctc agcccctata
      541 agatgctccc caccgccgcc ggctgcggca tggagcagaa gcacccgggc cggagggcgg
      601 cgctgtagga gttcaacatg ctcaaagaaa aaatcagccc gatggacctg agacatcccg
      661 agcacggcgc gtatattgcg tggctgcgca atctgacaat gcaggaacgg ggcgattaat
      721 tatggaaaaa tgtagtgaag ctgcagtcgc agagcgggcg cggattgctg cgggcctgcc
      781 acctacgcct ccagaaccga tccctgcatc cacgctggag ttttttcgtc gggcaatcgc
      841 acgacaacag gcgaaagagg cagccgctcc ctctgactcc gagtcgcccg cgtgagtgac
      901 gctgcgctac cgattaaaga cacgctgtcc ccggcgtcac atgacgaggt ggcagcggcg
      961 gtgcgcgcgg ctttcgatag cattacaccg ctctatccca tcggcggcgg gacggcgctg
     1021 ggattcggct tgccggcgaa ggcgccgggc cgggggctct cgctggcgaa gctgaatcgc
     1081 gtcgttgatt atccggcccg cgacatgacc gtcacggtcg aggcggggat cacgatgcac
     1141 gcggtcgggg agttgctggc gaaggagcgg cagcgattgc cgatcgacgt gccgcaggcg
     1201 gagcgggcga ccatcggcgg cgtgattgcg accaactgga acggcccgcg gcgattcggc
     1261 gaaggaaccg tccgcgacta cgtgatcggc atcagcgcag tcgatggtcg cgggcagccc
     1321 ttcaagggcg gcggccgggt cgtcaaaaac gttgccggct acgacttttg caagctgctc
     1381 accggctcgc tgggcaccct gggcgtcatc acgcaagtca ccctgcggct gaaacccatt
     1441 cccgagcaat cggcgctcgt cgcttgtgcc gttgccgatt ccgcggtcgc cgaaaagctg
     1501 gtcgcggggc tcgtaaactc caagacgact cccacggcca tcgagctcct ggccgggccc
     1561 gcctggcaga acgatccggc gctcgccggc ctgggggcgc tcgtcaagtc gagtctgtac
     1621 ctcgtcgtcg gcctggaggg aacgggaccg gaggtggaat ttatgacgcg gcagcttgcc
     1681 agtgaatggc gggctgcggg ggtcgattcg ctcgtcgtgg gcgaggcggc agctctgtgg
     1741 cggcggctgg tcgagtttcc cgccgaaggg gaatctccgc tggtgctcaa ggctagcgtc
     1801 gcccccagcg gcgtcgcacc tgtcatcgaa gcagcccgcc gcctcgaccc ggaatgttcg
     1861 ctccaggccc atgccggcag cggcatcgtg ctgatcaagc tgtcgaagtt cccagaacag
     1921 ggcctgtccc gcgcgctggt cggcaatttg cagccggcgg cagcggcgca ccttggctat
     1981 ttgatcgtgc tcagcaaccc ttcgggcgcg gagatgacgc atcagtgcgt gtggggcgca
     2041 accgatgtgc ccctgtggct gatgaccgag gtcaagcgga agtttgatcc gcaggacata
     2101 ctcaatcgtg gacggttcgt ttatttaggt agatcgcagt agccgtgtct ggctattgca
     2161 gcgtactacg atcttttcga ttgccggcgc aattcgttat tcggcagaaa gccacgatcc
     2221 tccaggcggc cgcgcaggcg cttgaggtcg ccggcccgac ctcaggcgca gtttgcagcg
     2281 cccgggtgga atctcaagtg gccggatcgt ctcaaaaaag tatctcagag tggtacggca
     2341 acgcacggag cgccggctgc cagcgggcta tactccccgc catgcagccc gcggaaacca
     2401 tgacccgcct caaccttctg accgacgaag gccccatagc ggtcttgttt actccggcgc
     2461 tcgaatcgac ccactactcc gagctgttcc gactgtccaa ggaattcgct agtgaggcag
     2521 atatgcgagc catcgtcgcc gctgccgcgg agcgatgggg ccgcgacgtg gttatcagca
     2581 gttacagctc aaattgaacg ccagagcccc actgcgttgc caaggcctct actgccttac
     2641 gcagttggtc ccgcgtcgtg gcctgctcgg ccgcgtgcag caactcccga tattgatcgg
     2701 cagtaagctt cgggtgcagg gccattcgca gcgcaccgtc acgcatcaag agttgcaacg
     2761 ccgtttccat gaccgatcaa cctagccctt gcagccgatc gtcgcaaggc gaatgctatg
     2821 tgcggtaacg tacagtaacc gactgtgcga gctcccccta cggtgcatag ctccaccatc
     2881 acgcccccgt cagcaccggc ggccccagcg ccaagacgat cagcagcgtg cggagtcggt
     2941 accgcatcat cgtttcaggt tcggcagggc gggatcgatt catcgagcca cgaaagcgga
     3001 gcgtaaatcc aagaaaccaa ccttcgcagg aaggcgggat gtagccgcgg ctggcgcacc
     3061 atgcgtatgg cccgaccgaa agcacgtaca gcgctggcaa cgcacgatca tggctgcgat
     3121 gaacggtgtc ctgccaggcg aggcggccag gcgggctgtc gggctctggc gtacgcggcc
     3181 tagcgggctg atttcaggta aaatagattg tggcctctgg cgacaggtcg cacagtctat
     3241 ttaccaaatg ccacgtatcg tggacggttc gtttacgcca gtctcgagca tcatgacgtc
     3301 gcccgccgcg gatgccgcgc cacaggccag cgcgcccgcg cttgcgcagc ccctacagga
     3361 aagcactgcg ctgcaactct cgcaaatcgg gccagcgccg caggccaacg gcgcgccggg
     3421 cgcggcgatc gactacgagc tgttcctcga ctgcgtccat tgcggcctgt gcacggcatc
     3481 gtgcccgacg tatgtcgaga cgggcaacga aaacgacagc ccgcgggggc ggatttacct
     3541 gatgcgggcc gtccgcgacg ggcggctgac gctcaatcac gaagtgcggc ggcacctgga
     3601 actgtgtctc gattgccggg cctgcgaaac ggcctgcccg tcgggcgtgc aatacggcaa
     3661 gctgatcgag ccgttccgcg tctcgatgga agggcacgcc ggaaccaact cggccaaggc
     3721 gaacgactgg ttccacaagt acattctgtt cggcctgttt ccttacccag agaagctgcg
     3781 gcaatcgctg cggccggccc gcgtcgcgca gtcgctcggg ctcgacaagc tgctgattgc
     3841 caccgggctg tggcggctgt tgccgccgcg gctgggccgg ctggtgacga tgctcccgcc
     3901 gctggaaaaa ccgctgccgg cgctgccgga gttcctggcg gccaaaggca agcggcgggc
     3961 ccgcgtggcc ctcttcacgg gctgcgtcgg cgacgtgatg ttccgccaca ccaactgggc
     4021 cacggcccgc gtgctgcaag aaaacggctg cgatgtcgtc gtcccgcagg cgcaggtctg
     4081 ctgcggagcg atccactttc acgccggcag cagcccgccg gcccgcgaaa tggcagacaa
     4141 gaatgtagcg gccttcgacg ccgctggcgt cgatgccgtg atcgtcaacg tcgccggctg
     4201 cggctcgatg ctcaaggact acgggcatca ttggcacgac gagcactcgg cggcccgcga
     4261 gcagtttgcc cacaaggtcc gcgactgcag cgagttcctg gaccagctcg gcctgatcga
     4321 cccgccgggc gaaattcgcc tcaccgcgac ctatcacgac gcctgccacc tggcccacgc
     4381 gcagaaggtc cgcgagcagc cgcgaaactt gctccggaaa attccgggcc tcaagctgat
     4441 cgatctgccc gaatcggacc tgtgctgcgg cgccgcaggc acctacaacc tgaccgagcc
     4501 cgagatggcc ggccgcctcg gcaagcggaa gctcgacaac attcgctcga ccggcgcaca
     4561 ggtcgtcatc actgccaacg ccggctgcct gctgcagatt gcccgcgaag cccgcctccg
     4621 cggcgagtcg ctcaagatca tgcacccgat ggaattgctc gacctttcct accagcgcca
     4681 aggcgtgcgg ttctagaacc agttccggat cagctccgca aagcggccga tgtccatcgc
     4741 gctggcgaac accatcagcg ccagcaggca gagcagcccc agcaaggtca ggcgatactg
     4801 cagttcgccg ctggcgggct tgccccgcac gccctcatag gtgaggaaca gcatgtgccc
     4861 gccgtccagc acgggaatcg gcaggaaatt cagcacggcc aggttggcgc tcaggatcac
     4921 caggaacatc aacagcgagc tgaacccttc cgaggcaaag cccgcagccg ccatcaggat
     4981 tcccggcggc ccggagagat tcgtcgccgg gagccgcccc gtcaccaagc tatacagaat
     5041 cacgaggact tcctcgacgc gctcgacggt ctcttgcgcg cccgcctgga acgcatcgcc
     5101 ccaattggtg ccgatttgtg tctgcgtaag cgcgtacgtt ttcaggccgc gccggtcgtc
     5161 gaagaatgtc gccgaatcgc gcggactcat cacggccgac atcgtctgct tgccgcggag
     5221 ccaggtcagc ttaatcttcg tgtcggccgg caccatttgc agccccacca ccgcatcgat
     5281 ccaggagtcc ttcgtctcgg caaattccag cgcgtcaaac cgaaacgagc cggcgatttt
     5341 cttgatcttc ttcacgtctt ccggccgccc gccggggacg aattccagct ttgtgacgaa
     5401 gtcgccagca cgcagaccgg cggcgcccgc cggcccggcg gaatcgacgc tgttcacttc
     5461 gttcatcacc gcgaaggcca cgccgatcga ttcaatcgac gtcgggttca tcgccggcgg
     5521 gtgcagcgcc gattgaagcg gcggctctgg agtaacttcc agcgtcttcg taacgggctg
     5581 tccttgccga tccttgcgct gcacggtgat cttgagcggc tcggtgctgc ccggcttggg
     5641 tgtcaggcgc tgcgagagcg acagcggatc acccggcggc tcgccgtcga tctccagaat
     5701 ggtgtcgtcc ggcaagaggc ccgccaattg cgccggcgag ttcttacgga ccgccacgat
     5761 cgggcccatc ttcatcgtca ggcccaactc gcgcatcggc cgcggctcga gagtgacatc
     5821 cacaaactgc ggctgccgct tcgagtcctt gaccagttcg ccgctgggct ggcgctcgcg
     5881 gcgctcgact ttgattgcga tctgccccag cggctgctgg gcgagcaccg cgcctagttc
     5941 ggcgccgctc gcgaccttct ggccggcggc ctcgacaact ttgtcgcgct ccagcagctg
     6001 ggcggaggac ttgggattca agtacgaggc tgggccgggc cagatattga gctccaagtc
     6061 ctcggccggc gcgatgccga tcatcggcga cttgccgccg ggtttcgtcg tgggcgtcag
     6121 ctcgtgccag tcttccttgc cgtcgggctc tcggaccagc agcttcaccg ggcggccgtt
     6181 gtttagcatg gccgtctgcc ggaaatcggt gaagctcagg aattcattgc gcctcccccg
     6241 ttcgccgatc tgaatcagcc gcgatccggg ctgcaatccc tcgacccacg ccgcgccgcc
     6301 tggcgaagtc gcgccgatga ccgcgggcat ctccttgacg cccaggcgaa acgccaccgt
     6361 ggccatgatc accgcgaaga tcaggttcat gatcacgccg gccgagatga tcgccatccg
     6421 cgccaggacg ctcttggcgg gaaagctgcg cgggtcgagc aggatcgtgc ggcccgcagt
     6481 cgtctgagcc gggaccggtg gatcggcggg cttgtccttg tcgtggacgt gagcagctgc
     6541 cagagcttcg ttggtcaact tctcgacgga ctgaccggcg acgaggccct cggctgcgtc
     6601 gccgctggcg atcgcttcga cgcgggcatc ggacgcggcc ggcgccgcgg tgcgaatgcg
     6661 agccgcttcg gcctcggcgt tccgcgggtc gtcgtcctgg cccagcatct tcacatagcc
     6721 gcccagcggc aggctgccca ggccgtattc cgtctcgccc cactggaact tgaacagcga
     6781 gcggggcagc ttccacttgg tgaacggaat cgggatctcg aaaaagtcga agccgacgta
     6841 gaacttctca cacttcaccc cgcaggcctt ggcgacgagg aagtggccca gctcgtggac
     6901 gaagatcacg aacgtcaggc cgccggccac gcctagaatc atccagaccc aggtgccgat
     6961 catttccaac caggttggct cgaccgccga taccgcgagc atcaggcaac tatccaccgt
     7021 tgcatctcct gccgggccca gccgtcgagc accagcagct cgtccagcga aggttcggcg
     7081 tcaaagtgat gactttccag tacggcccgg caagccggga cgatctccat gaacggcatc
     7141 tcgccggcca ggaagctggc gaccgccgcc tcgttggccg cattgagcac tgcacccgcc
     7201 gtaccgccgg ccgccgcgac ctcgcgcccc agcttgagag ccggaaagcg gtcctcgtcg
     7261 gggggctcga actccagccg caaggcttct gtccagtcga gccgcctcgc cggggacggc
     7321 tgccgctcgg gccaggtcag agcatactgg atcggcagct tcatgtcggg cgggctcagc
     7381 tgggccagca ccgaaccgtc cacgtattcg acgagcgaat ggacgaccga ctgcgggtgc
     7441 acgacgactt cgatctgctc gggcggcaag tcgaacagcc accgcgcctc gatgatctcc
     7501 agcgccttgt tcatcatcgt ggccgagtcg atcgtgattt tgtcccccat ccgccaggtt
     7561 ggatgcgcca gcgcgtcggc cacggtcgct gtctccatct gctcgcgcga ccagcagcgg
     7621 aacggaccgc cgctagcggt caggatgatc cgcttcaaag ccgcgaccag tggtcgcggg
     7681 ggggtcggtg ggtcgtccgc tgtgcaccgc gaccactggt cgcggctttg gccctccagc
     7741 aactggaaca ctgcactatg ttcgctatcg accggcagaa tcttggctcc ccgctgggcc
     7801 gacagccgca tgacttgcgg gccggccatc accagcgtct ccttgttcgc caacgcgacc
     7861 ggcttacccg cgtcgagggc ttcccacgta ccggtcaacc ccgcactgcc gacgatcgcc
     7921 gaaagcacaa tgtccacttc aggcagccgg acgattcggg ataatccctc gggtccaatc
     7981 agcggctccg tttcccgcgg cagctgggac caatcaaagc cccgggcggc ctctgcatcg
     8041 gtggccacga caaaccgcgg ccgcagctgg cgggcctgct tgcagaggtc ggccagtcgc
     8101 tgccttgccg ccagggccac aacttccagc cggccccgcg aagccgccac gacctccagc
     8161 gtgctctggc cgatgcttcc ggtcgagccg agaagagcga ttctgcgagg ggggtgatcc
     8221 atagcgggcg gcaggcggcg tggggccgag gaatcagcag cttattcctt gccaggttcg
     8281 gccggcatag caacaatgaa cctaaccacg tcgcgaagcc tgcgtcgcca gcccgaaccg
     8341 gacattccct gtccggcctc tggcacgccg caaggaactc gacctaactt gtgtgctcgg
     8401 caagagttcc ggctcgccgg gtattgtagg gacgtaagcc aagcccggca accacatcaa
     8461 tttacggtca gggtgcggtg gtgggtcttg cgcaggcagg caaacctacc ttgcccgccg
     8521 gaggatgcgt cggcgggcgt cccgatacgt attggcgaat ctgtagttca ggactgtgag
     8581 atatgtcgca agacaacggc tccggccggc aaaaacccga aggaaatggc tcgtccggct
     8641 cgaacctctt gctctgggcg gggttgatcg tcgccaccgt gctgctcgtg gtcctgtggg
     8701 tcacgccctg gttgacgaaa gaattgcggc cagaagacct gcaagccttg atcaaggctg
     8761 gcgccaagga caccagcggc aatccggtca acacggttga atacgtcgat gtgcgggccc
     8821 ccaacaacaa gtactatcgc tattcaaagc tgcagaaggt cgtcatccgc gagcggtcga
     8881 tcaccgggca ggtggacctc gtcgagctgg tccagcaggg cgacggggag gccaaaaagc
     8941 tcgtccccaa cggcaagacg agatcggagg cccaggcctt ccgcaccgac attgatcccg
     9001 aaggcgagta tcgcgcgcag ttccccaagc tgctccgcga gaacaacttc accgattgga
     9061 agttcgacac gacgcagaac acctgggagc agcacacgcc ctggattctg ttcacgctcc
     9121 tggcgatctt cttgatctat ttcatgctcc gccgcttgat cggcgcgggc tcgccgatgt
     9181 cgttcggccg cagccgcggc aagctctatg cccaggaaga cctgggcgtg acgtttaacg
     9241 acgtggcggg catcgacgag gcggtcgagg aagtccgcga ggtcgtcgat ttcctccgct
     9301 cacccgacaa gtaccagcgg ctgggcgggc ggattcccaa gggcgtgctg ctggtcggcc
     9361 cgccgggcac cggcaagacg ctgctggcca aggcgatcgc cggcgaggcg ggcgtgccgt
     9421 tcttcagcct ctcgggctcg gacttcgtgg aaatgttcgt cggcgtcggc gcggcccgcg
     9481 tccgcgacat gttcacgcaa gccgaagcca aggccccctg catcatcttc attgacgagc
     9541 tggacgccct cggcaaatcg cgtggcacca gcattgtcgg cggccacgac gagcgcgagc
     9601 agacgctgaa cgccctgctc gtcgaaatgg acggtttcgg cagcaatagc ggcgtgatcg
     9661 tgatggccgc gacgaaccgg ccggaaacgc tcgacccggc cctgctccgc gccggccgtt
     9721 ttgatcggca ggtgctcgtc gatcggcccg atatccgcgg ccgcgaagcg atcctcaagg
     9781 tccacgtcaa gaacgtgaaa ctcgacccga ccgtggaact gaaggaggtc gccgcgatca
     9841 cgcccggctt cgtcggggcg gacctggcga atttggtcaa cgaggcggct ctgctcgcgg
     9901 cccgcggcga gaagaacgcc gtcgggatga aggagttcaa cgaaggggtc gagcgcgtca
     9961 ccgcc
//
LOCUS       DASRJB010000561         5931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_157600, whole genome shotgun sequence.
ACCESSION   DASRJB010000561 DASRJB010000000
VERSION     DASRJB010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157600"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VFB80_14650"
     CDS             complement(<1..1503)
                     /locus_tag="VFB80_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN35065.1"
                     /translation="MRQRHSGTSTRRLVLETLEDRSLLAAVASDGRRLGAPVATPAAL
                     IDDSFEENDSRLAARNLGTLTASATFNNLVMADAADWYRFSTTLTGTSSDNVRITFLH
                     AQGDVDMELYNASGIRLRYSNGVTNAEQISLSGLAAGTYYVRVYGYLGVFNPSYSLTI
                     NPPRAGAADDAYENNDTLGTARDLGTLAAAQTISNLVMADSHDWYRFSMSGPGAAGDL
                     VAINFQQTQGDLQLELYSSGGAPLGSSLGTASGESISLAGLAAGTYYVHVFGAGGALN
                     PNYSLTIDPGTAAAPPPPPPGTGGFDIQFSFSGLTASQQSIFEQAAQRWESIIVGDLP
                     NASYNFLPVDDLLIGASSVFIDGSGGVLGQAGYDRLRSATAGGLPYHGIMQFDSADLA
                     SMQANGTLLGVIAHEIGHVLGIGTLWSNRGLLVGASTSNPIFVGPQATAEYNALFGTT
                     AAGVPVENTGGSGTRNSHWRESIFRNEIMTGWVGPGTSLPISRMTVASLAD"
     gene            complement(1884..2324)
                     /locus_tag="VFB80_14655"
     CDS             complement(1884..2324)
                     /locus_tag="VFB80_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35066.1"
                     /translation="MNRATDTFRSRFGYHPCDFATYQKLKSLKKWYWQSVKDFHRWWR
                     WQRKRPENRRGPEPAFCPVFVRNEPWFKPRRVHGQDAVRYYPKTLIEHGVLELLALAR
                     QPQAEPPTPLDESHLRVIDRLHAAAAEWFEVGSACRAEPQHGDA"
     gene            2357..2776
                     /locus_tag="VFB80_14660"
     CDS             2357..2776
                     /locus_tag="VFB80_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35067.1"
                     /translation="MQAAVAAQRPALVAAERRCAGGGELAAEIGLAERAVVDRQARQA
                     RDDAVERQFRGNATEHQMRMLARGRDQRVPRGFNRGVCGLDGLLRRRQIRADEQINVR
                     RLNLRDLRETNASHEGLLSKWHERELVNAAGKGRAVP"
     gene            3028..3690
                     /locus_tag="VFB80_14665"
     CDS             3028..3690
                     /locus_tag="VFB80_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35068.1"
                     /translation="MRCELVLAVVLCAGFVAPALAEDKPVRKAGEGKGDQAALQQKLL
                     QQFDANKDGVLSDQEKLAANEFLRRSGAVAGVAPGGFPGADQFAKQFDKDGDGKLNDQ
                     EKLMAQAAWQRMRARSSGPTYGPGYAGPYGFAQANANAGGVAAANAAAAGGGAGGEGK
                     AKVSALVKRFDKDGDGKLNDEEKAAAQAELKKDKKSKADKPMDEKPAEEKPAEKPAAE
                     KP"
     gene            4285..>5931
                     /locus_tag="VFB80_14670"
     CDS             4285..>5931
                     /locus_tag="VFB80_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN35069.1"
                     /translation="MVGLLLGVLVGQVSTGGERQIDFQKDVAPILQQQCLDCHGPALQ
                     MAELRLDQRKFVLADDGRGLVKPGKSSESLLVHRLTDRKLGILMPPTFPFFPGEKAGL
                     AEEKIKVLQAWIDQGAQWPEGVTLASETASAENAKVRALFVAIRSGRHQAVRDLLDKD
                     KDLASARAADGSTPLMQAAAYADAQLMQLLLDRGPDVNAKNRDGATALMRAAGDLEKV
                     KLLLARGADLQAKSALGRTALLIAATYPGNPATVKLLLDRGANIKDVDSYGETCLTSA
                     CKRGDAEMAKLLLAAGADPIAGGRPPLVWAAEEGNLATIACLLEHGTPQPPPVISAAL
                     SSAAARGPAEAVKLLLARGADPNAPSRIAGYTPLMWASYSDYQSDNAVKLLLDKGADR
                     GGKGADGQTPLSLARKFGATPIVALLEQGRAGEPAPEAAAGDGAQLPELRAAIDKSLK
                     LLQECGPKFFNQSGCIACHQQSVTSLAVAAARRKGVKVDEQTAREQVAMTALTAKTYI
                     ERFLQRADHPAGSAPSGGYISLGLAAEKYPADEITDATIFE"
BASE COUNT         1080 a         1916 c         1978 g          957 t
ORIGIN      
        1 gtcggctagc gacgcgacgg tcatccggct gatcggcagg cttgtgccgg ggccaaccca
       61 gccggtcatg atctcgttgc ggaagatcga ctcgcgccaa tgcgagttgc gggtgccgct
      121 gccgccggtg ttctcgacgg gcacgccggc cgcggtcgtc ccgaacaggg cgttgtattc
      181 ggccgtcgcc tgcgggccga cgaagatggg attgctggtc gaagcgccga cgagcagccc
      241 gcgattcgac caaagcgtgc cgatgcccag cacgtgccca atctcgtgcg cgatcacgcc
      301 cagcagcgtg ccattggcct gcatcgacgc caggtcggcc gaatcgaact gcatgatgcc
      361 gtgataaggc aagccgccgg cggtggcgct gcggagccgg tcatagccgg cctggccaag
      421 tactccgcca ctgccatcga tgaagaccga gctggcgccg atcagcaagt catcgaccgg
      481 caagaagttg tagctggcgt tcggcaagtc gccgacgata atcgactccc accgctgcgc
      541 cgcctgctcg aagatcgact gctggctcgc cgtcaggccg ctgaagctga actggatgtc
      601 gaacccgccc gtgccgggcg gcggcggagg cggcgcggcg gcggtcccag gatcgatcgt
      661 cagcgaatag ttcgggttga gtgcgccgcc ggccccaaaa acgtggacgt agtacgtgcc
      721 ggccgccaga ccggcgagcg agatcgactc tccgctggcc gtacccagcg acgagccgag
      781 cggcgcgccg ccgctgctat acagctccag ctgcaagtcc ccctgcgtct gctggaagtt
      841 gatcgccacc aggtcgccgg ccgcgcccgg accgctcatc gagaagcgat accagtcgtg
      901 gctgtcggcc atgaccaggt tcgagatcgt ttgtgcggct gcgagcgtgc ccaggtcgcg
      961 ggccgtcccc agcgtgtcgt tgttctcgta agcgtcgtcg gccgcgcccg cccgcggcgg
     1021 attgatcgtc agcgaatacg agggattgaa cacgccgagg tagccataga cgcgaacgta
     1081 atacgtcccc gccgccaggc cgctcagcga aatctgctcg gcgttcgtca cgccgttgga
     1141 ataccttagc cggatgccgg acgcgttata taactccatg tccacatcgc cctgcgcgtg
     1201 cagaaaggtg atgcggacgt tgtcgctgct cgtgcctgtc agcgtcgtgc tgaaccggta
     1261 ccagtcggcc gcgtcggcca tgacgagatt gttgaaggtc gccgaggccg tcagcgtgcc
     1321 caggtttcgc gccgccagcc ggctgtcgtt ctcctcgaag ctgtcatcga tcagcgccgc
     1381 cggcgtcgca accggggccc ccagccgcct gccatccgac gctaccgctg ccagcaggct
     1441 gcgatcttcc agtgtttcca gcacgagccg tctggtcgaa gtccctgagt gacgctggcg
     1501 catcaaacgc tccctgtgtt gcgctgcaag cagcacgctg tggtgtcgct tgcgcgtgtt
     1561 cgagaagagg tctgtcccgt cggtccgccg gaatcggcgc cgagaaactg ctgcatcatc
     1621 gcgatgcaag cgagttccag caagcgaatg gcgaaaatgt aaaataccgg acgtgctagc
     1681 aggggttgtc actctgccac tctcgcgcca gggcgctcac cctttcaggt gccagtgcgc
     1741 tgaaccacgc cgatagcgcg tagcagtgac tgcgccgggg ctcgaacccg gatcagcgat
     1801 tttagagatc gccgctctga cctgttgagc tacgcagtca cgtaggtccc gcctgccggg
     1861 cgggacctgt tcttcccagt ggatcacgcg tcgccgtgtt gaggttccgc ccggcaggcg
     1921 gaacctactt cgaaccactc ggccgcggcc gcgtgcagcc ggtcgattac ccgcaagtgg
     1981 ctctcgtcca acggcgtggg cggttcggcc tgcggctgcc tggccagggc cagcagctcc
     2041 agcacgccat gttcgatgag cgtcttggga taatagcgca ccgcgtcctg cccgtgcact
     2101 cgccgcggct tgaaccaggg ttcgttccgc acgaagaccg ggcagaacgc cggctccggt
     2161 ccgcggcggt tctcgggccg cttgcgctgc cagcgccacc agcggtgaaa gtccttgacc
     2221 gattgccagt accatttctt cagcgacttc agtttttgat aggtggcaaa gtcgcacgga
     2281 tgatagccaa agcggctgcg aaaagtgtca gttgccctgt tcattgagtt cgtcctgttg
     2341 gatttgaggt tggagattgc aggcagccgt agcggcgcag cggccggcgc tggtcgccgc
     2401 ggagcggcgt tgtgcaggcg gcggtgagct ggccgccgag atcgggctcg cggaacgtgc
     2461 ggtggtcgat cgccaggcgc gacaagcgcg cgacgacgcc gtggagcgcc agttccgagg
     2521 aaacgccaca gagcaccaga tgcggatgct cgcccgtggg cgggatcaaa gagtgccgcg
     2581 cggcttcaat cgcggcgtgt gcggcctgga cggcctgctg aggaggaggc aaatccgtgc
     2641 ggacgaacag ataaacgtac gccgtctcaa cctgcgggac ctacgtgaaa ccaatgccag
     2701 tcatgaaggg ctcctgtcaa agtggcatga aagggaactt gtgaatgccg cgggcaaagg
     2761 ccgtgccgta ccttgattgg cgagcgggag gcgtaagcct cccgatagcg ccgcgcaccg
     2821 acagccaccg attatcgaga caactttgac atcgggaggc tgacgcctcc tgctcgccgg
     2881 tccctgcggg agggaggagg cacaattctc ctccaagtcc tagcaattcg ctggaatctg
     2941 tgagtacaat caagcgttgg agagcattcc aactgcgtgc cgccaggggc aagtcaggcg
     3001 ggccagctat tttgtgagcg acgtaacatg agatgcgaac tggttttagc ggtcgtgctc
     3061 tgcgccggtt tcgtggcgcc cgccttggcc gaagacaagc cagtccgcaa ggctggcgaa
     3121 ggcaagggcg atcaggcggc cctgcagcag aagttgcttc agcagttcga cgccaacaag
     3181 gacggcgtgc tcagcgacca ggagaagctg gccgccaacg agttcctgcg gcggtccggc
     3241 gccgtggccg gcgtcgcgcc gggcgggttt cccggcgctg accagtttgc caagcaattc
     3301 gacaaggacg gcgacggcaa gctcaatgac caggagaagc tgatggccca ggccgcctgg
     3361 cagcggatgc gggcgcgcag cagcggtccc acttacggcc ccggctacgc tggtccctac
     3421 ggcttcgccc aggccaatgc caatgctggc ggagttgccg cagccaacgc ggctgccgcg
     3481 ggcggcggcg ccggcggcga agggaaggcc aaggtctcgg ccctcgtcaa gcggttcgac
     3541 aaggacggcg acggcaagct gaacgacgaa gaaaaggccg ccgcccaggc cgagctgaag
     3601 aaggacaaga agagcaaagc cgacaagccc atggacgaaa aacccgcgga agagaagccc
     3661 gcagagaagc ccgccgctga aaagccctag ctccaagccg cgaccagtgg tcgcggtcta
     3721 accccccgcc attgccaggg cggcaatgac cagcgccaga atcgtccctg cgattgccgc
     3781 gcgcacgatt cggaccgcca atagatcttg accggtggct ctgaccggca atcgaaagct
     3841 gggatcgaag caaggcatag cgcagcccta cataagcggg gaaaccaccg ggctctttcc
     3901 caagcgcatt cactctcccg ttgttacgcc cggtggcgac gcagctcgcg tcgtcgcctc
     3961 cggctccgta agtaaacatt ggtacgctat ttgcactaca gttccaagtg gcgcgccagg
     4021 ctgccgtttt ggccgttgcg tcgcgcgggc cgagcgccga atcgagccgg ctgccaggcc
     4081 ctcaaccacg catacggagc cctcaaacac tcatatataa ctacttaacg gggtagtgag
     4141 aatttgtccc aagtcgatgg gctgccggac tttggggcaa acgacgcgct ttgcaagcca
     4201 ctccaccggg ggtcgccctt ggcgatttcc ggttcgtccg tatgatgtcg ctccagcggg
     4261 cgtcatctgc aacaggaggc aggaatggtc ggtttgctgt tgggcgtgct ggtcggacag
     4321 gtttctacag gaggcgaaag gcagatcgat tttcaaaagg acgtcgcgcc gatccttcag
     4381 cagcagtgcc tcgattgtca cgggccggcg ctgcagatgg ccgaattgcg gctggaccag
     4441 cggaagttcg tgctggccga tgacggccgc gggctcgtca agccgggcaa gagcagcgag
     4501 agcctgctgg tgcatcggct gaccgatcgc aagctgggca ttttgatgcc gccgacgttc
     4561 ccgtttttcc ccggcgagaa ggcgggcctg gcggaagaga agatcaaggt gctccaggcc
     4621 tggatcgatc aaggcgccca gtggccggaa ggcgtgacgc tcgccagtga gacagcctcg
     4681 gccgaaaatg ccaaggtgcg ggcgctcttc gtcgcgattc gcagcggccg gcaccaggcc
     4741 gtgcgcgact tgctcgacaa agacaaagac ctggcgagcg cccgcgccgc cgacggcagc
     4801 acgcctctga tgcaggccgc agcctatgcc gatgcgcagc tcatgcagct gctgctcgac
     4861 cgcggcccgg acgtgaacgc caagaatcgc gatggcgcga cggccctgat gcgggccgcc
     4921 ggcgacctcg agaaagtcaa gctgctcctg gcgcgcggcg ccgatttgca ggccaaatcg
     4981 gccctcggcc gcacggcgct cctgatcgcc gcgacttatc cgggcaatcc cgcgacggtg
     5041 aaattgctgc tcgatcgcgg cgcgaatatc aaagatgtcg attcctacgg cgagacctgc
     5101 ctgaccagcg cctgcaagcg cggcgacgcc gaaatggcaa agctgctgct ggctgctggg
     5161 gccgacccga tcgccggcgg ccgtccgccg ctggtctggg ccgcggaaga gggcaacctg
     5221 gcgaccatcg cctgcctgct ggaacacggc acgccgcagc cgccgccggt catcagcgcc
     5281 gccctttcat cggccgcggc ccgcgggccg gccgaggcag tgaaactgct gctggcgcgc
     5341 ggcgctgatc ccaatgcccc gtcgcggatt gccggctaca cgccgctgat gtgggcgtcg
     5401 tattccgact accagagcga caacgccgtg aagctgctgc tcgacaaggg cgccgaccgc
     5461 ggcggcaaag gcgcggacgg ccagacgccg ctcagcctgg cccgcaaatt cggcgcgacg
     5521 ccgatcgtcg cgctcctgga gcagggcagg gcaggggagc ccgcgccgga agccgccgcc
     5581 ggcgatggcg cgcagctgcc ggaactgcgt gcggcgatcg acaagagcct caagctgctg
     5641 caggagtgcg gtcccaagtt cttcaaccag tcgggctgca tcgcctgcca ccagcagtcg
     5701 gtgacgtcgc tggcggtggc cgccgcacgc cgcaagggcg tgaaggtcga tgagcagacg
     5761 gcccgcgagc aggtggcgat gaccgcgctc accgccaaaa cctacatcga gcggttcctg
     5821 cagcgtgccg atcacccggc cggcagcgcg ccttcgggcg gctacatctc gctcggactg
     5881 gcggcggaga aatatcccgc cgatgagatc accgacgcca cgatcttcga g
//
LOCUS       DASRJB010000562         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2414130, whole genome shotgun sequence.
ACCESSION   DASRJB010000562 DASRJB010000000
VERSION     DASRJB010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414130"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFB80_14675"
     CDS             complement(<1..204)
                     /locus_tag="VFB80_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35070.1"
                     /translation="MAKRPIIAKADRESGPGAELLALCQRITEHGKLTEEGIKELIRW
                     LRNNKAVNLPAKDYLTEILSQIAA"
     gene            complement(212..1603)
                     /gene="asnS"
                     /locus_tag="VFB80_14680"
     CDS             complement(212..1603)
                     /gene="asnS"
                     /locus_tag="VFB80_14680"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZN35071.1"
                     /translation="MEKISVKAARQPAAIGQSVLVQGWIRTRRDSKGGFSFLEVNDGS
                     SLANLQVIAEGSLPNYESEVKHLVAGCSVAIEGEVKASGGKGQATEVLAKKLTVLGTA
                     DPEAYPLQKKQHSLEKLREWAHLRPRTNTFGAVTRVRNCVSRSIHNFFQEDGFLYIHT
                     PIITASDCEGAGAMFRVTTLDLDKLAASGTKLNPALDFFGRPSYLTVSGQLEGEIFAC
                     SLGKIYTFGPTFRAENSNTSRHLSEFWMVEPEMAFYELADNMDLAERFLKRIFKDALE
                     QCPEDMAFFNEHYDKTTLETLQKIIATDFRRLPYTEAVEILEKCGEKFEFPVKWGIDL
                     QSEHERYLTEKHFQGPVILYDYPRTLKPFYMRCNDDNKTVRAMDVLVPKVGEIIGGSQ
                     REERLDVLTQRMQDQHLKPEDYWWYLDLRRYGTVPHAGFGLGLERAVQFLTGMANIRD
                     VIPFPRTPGSAEF"
     gene            1785..3617
                     /locus_tag="VFB80_14685"
     CDS             1785..3617
                     /locus_tag="VFB80_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35072.1"
                     /translation="MTKKPIMQPDKGAMRSAFAPYTRRELVLLGVVLAAGVAARVWAV
                     SRSAVEHFDEGVYASNVYFGEPDYAYPLQRYYAPPLLPALIEAGMIAGLPPNLAAMLP
                     SFLAGCGTIAALWWFGRSWFGPEVGLAAAALVALNDYHITFSATALTDVLLGLWLVLA
                     VDAIGRSLVGERPGVSRPIAPAGDKRGRERERHREADASRSPRGDYRWAIVAGIYTGL
                     AWWTKYNGWLPLAIEAAALPVLWLLLRPPARQLQSWLGCCAITAVVAAAVWSPYYFSL
                     QSHGGYGPIAANHAKYVVGLAGWFQSAARQIGQQYSFGQWQTAAGVVLAFALPTLLQR
                     RAWRDWVWRLVAGLGVAIAGLCLFSVLVVSVGAAIGLLRFLVALHRTPKLDDAWNRRA
                     IGFCLVAAWWSGMLVATPLYHPYPRLALPLMLAAWLGLAINTGDVVIETEQDFYETRL
                     GKFWSGLSLLVVAGMLLVFALFMHRRELPSDRRGVQHIAQQIHASDPASRQRVVYVYG
                     EPALFFQLRAAGEEIVSTVQQVPTAAATLEDKPIPTFLIAGPHAQRDPAFVRQFAAAK
                     ERWELVKEFEYRPSAVVWLDLNDPRHTLKETADLDRVRLYRLRP"
     gene            3823..>4715
                     /locus_tag="VFB80_14690"
     CDS             3823..>4715
                     /locus_tag="VFB80_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35073.1"
                     /translation="MARLESPPAAAATKPLLPAPGSAGGAGKWMALAAALLGWMFDGA
                     EMGIFSMVGRPAVKDLLSAQLSSLSVADQEKVIGLWFGIITAGFLVGAATGGVLFGWL
                     GDRIGRVRAMTLSVLTYALFTGLCGVVGEAWQVGILRFIASLGMGGEWSLGVALVMEV
                     WPNKSRAFMAGLIGAAANVGYLLVGFAGLGLLALIGTFKGWMLGAGMPDAWVEMLVRN
                     NGWRLMMLLGTLPALLTFFIRIFVPESEKWEKEKQKGSAGHWATQDLLGILVGIAGPA
                     LIVYVWATPQSPYLRFAATLLG"
BASE COUNT          720 a         1377 c         1648 g          970 t
ORIGIN      
        1 ggcggcgatc tgggagagga tctccgtcaa gtagtctttg gccggcaagt tgacagcctt
       61 gttgtttcgc aaccaacgaa tgagctcctt gatgccttcc tccgtcagct tgccgtgttc
      121 cgtaatccgc tgacaaagcg ccaacagttc agcgcccgga ccggattcac ggtcagcctt
      181 cgcgataatc ggtcgcttcg ccatttcgca cctaaaactc tgcactcccc ggcgtacgcg
      241 gaaacggaat cacgtcgcgg atgttggcca tgccggtgag gaattggacg gcgcgctcga
      301 ggccgaggcc gaagccggcg tgggggacgg tgccgtagcg gcggaggtcc aagtaccacc
      361 aatagtcttc gggcttcagg tgctggtcct gcatccgctg ggtgaggacg tccagccgct
      421 cttcgcgctg ggagccgccg atgatctcgc cgactttggg cacgaggacg tccatcgccc
      481 gcacggtttt attgtcgtcg ttgcagcgca tgtagaacgg cttcagcgtg cgcgggtagt
      541 cgtagaggat gaccgggccc tggaagtgct tttcggtgag atagcgctcg tgctcggatt
      601 ggaggtcgat gccccatttc acggggaatt cgaacttctc gccgcatttt tccaggattt
      661 caaccgcttc ggtgtagggc agccggcgga agtcggtggc gatgattttc tggagcgttt
      721 ccagcgtggt cttgtcgtaa tgctcgttga agaaggccat gtcttccggg cactgctcca
      781 gggcgtcctt gaagatccgc ttgaggaacc gctcggcgag gtccatgttg tcggccagct
      841 cgtagaaggc catctcgggc tcgaccatcc agaattcgga gaggtgccgc gaggtgttgc
      901 tgttctcggc ccggaaggtg gggccgaagg tgtagatctt gccgagcgag caggcaaaga
      961 tttcgccttc gagctggccg ctgaccgtga ggtagctcgg gcggccgaag aagtcgaggg
     1021 cggggttgag cttcgtgccg ctggccgcga gcttgtcgag gtcgagcgtc gtcacgcgga
     1081 acatcgcccc ggccccttcg cagtcgctgg ccgtgatgat cggcgtgtga atgtagagga
     1141 agccgtcttc ctggaagaag ttgtggatgc tgcggctgac gcagttgcgg acccgcgtga
     1201 cggcaccgaa ggtgttggtc cgcggccgca agtgggccca ttcacgaagt ttttccagcg
     1261 aatgctgctt cttctgcagt ggataggcct cggggtcggc cgtgccgagc accgtcagct
     1321 ttttggcgag cacttccgtc gcctgcccct tgccgccgga ggctttcacc tcgccttcga
     1381 tcgcgacgct gcagccggcg acgaggtgct tgacctcgct ttcatagttc ggcagcgagc
     1441 cctcggcgat gacctgcaga ttcgccaggg acgagccgtc gttgacttcg aggaagctga
     1501 agccgccctt gctgtcgcgg cgggtgcgaa tccagccctg cacgaggacg ctctggccga
     1561 tcgccgccgg ctgccgggcg gctttgacgg agattttttc catgggatgg ttttgaggag
     1621 cacggatgat acatggagga acacggattt taggggacag gaggcggcgg gagaatctca
     1681 cacagaggcg cagaggcaca gagagaggaa tgaattctct gtgcctccgt gcctctgtgt
     1741 gagcctatga tcggagtagc aacgcggcga tccggatgtg ctccgtgacc aagaaaccaa
     1801 tcatgcagcc cgacaaaggt gcaatgcgct ccgcttttgc accctacacg cggcgcgaac
     1861 tggtgctgtt gggcgtcgtg ctcgcggcgg gagtggcggc gcgggtgtgg gcggtgtcgc
     1921 gttcggccgt ggagcatttt gatgagggcg tgtatgcgtc gaacgtctat ttcggcgagc
     1981 cggactatgc ctatccgctg cagcggtact atgcgccgcc gcttttgccg gcgctgatcg
     2041 aagcggggat gatcgcgggc ctgccgccga atctggcggc gatgctgccc agctttctcg
     2101 cgggctgcgg aacgatcgcg gccctgtggt ggtttggccg ctcgtggttc gggccggaag
     2161 tcggcctggc agccgcggct ctggtggcgc tgaacgacta tcacatcacg ttcagtgcga
     2221 cggcgctgac cgacgtgctg ctgggcctgt ggctggtgct ggcggtggac gcgatcggac
     2281 ggtcgctcgt cggcgagcgg cctggcgtta gccggccgat agcgccggct ggagacaagc
     2341 gtgggagaga gagggaacga catcgggagg ctgacgcctc ccgctcgccc cgcggcgatt
     2401 accgctgggc gatcgtggct gggatctaca ccggcctggc ctggtggacg aagtacaacg
     2461 gctggctgcc gctggcgatc gaagcggcgg cgctgccggt actgtggctc ctactgcggc
     2521 cacctgccag gcaactgcaa tcctggctgg gctgctgcgc gatcacggcc gtcgtggcgg
     2581 cggccgtttg gagtccgtat tacttctcgc ttcagtccca cggcggttac ggccccatcg
     2641 cggcgaacca tgcgaagtat gtcgtcggcc tggcgggctg gttccaatcg gccgcgcggc
     2701 agatcgggca gcagtactcg tttgggcagt ggcagacggc tgctggcgtc gtgctcgcgt
     2761 ttgcgctgcc gaccttgttg cagcggcgag cctggcggga ctgggtttgg cgattggtgg
     2821 ccggtcttgg tgtggccatc gctgggctat gcctgttcag cgtgctggtc gttagcgtcg
     2881 gcgcggcgat tggcctcttg cgatttctcg tggccttgca ccgcacgcca aaactcgacg
     2941 acgcctggaa caggcgggcc atcggctttt gcctggttgc ggcctggtgg agcggcatgc
     3001 tcgtggcgac gccgctttac catccctatc cacgcctcgc tctgccgttg atgctcgccg
     3061 cctggttggg cctggccatc aacaccggcg acgttgtgat tgaaacggaa caggactttt
     3121 atgaaacacg actgggcaag ttttggagcg gcctctcctt gctggttgtg gcaggcatgc
     3181 tgctcgtatt cgccctgttc atgcaccgca gggaattgcc cagtgaccgc cgcggcgtcc
     3241 agcacatcgc gcagcagatc catgcttctg atccagcaag ccggcagcgc gtcgtctacg
     3301 tctatggcga gccggcgctg ttttttcaac tgcgggccgc gggcgaggag atcgtgagca
     3361 cggtgcagca ggtgccgacg gcggctgcga cgctggagga caagccgatc ccgacgttcc
     3421 tgatcgccgg gccgcacgca cagcgcgatc cagcgttcgt gcggcaattt gccgcggcga
     3481 aggagcgctg ggaattggtc aaggagttcg aatatcgacc cagtgccgtc gtgtggctcg
     3541 atctaaacga cccgcgccat acgctcaagg agacggccga cctggaccgc gtgcggctgt
     3601 atcgattgcg gccttgattc gagtgcagac aaggaggtta gccgcggatt tgcgcggatg
     3661 acacggatcc ggcagcggca tttccgatcc gcgtgatccg tgtttatccg cggcaattcc
     3721 tctccgttga ttcgagcagt cctaaggcgc gagttgcggc ccggcaacaa tcaaccgtag
     3781 aatcgacgct cccgcccgcc tttccctctt ccgagtttgc tcatggctcg cctggaatct
     3841 ccgcctgctg cggctgcgac gaagcccctc ctgccggctc ccggctcggc gggcggcgcg
     3901 ggcaagtgga tggcgctggc cgcggccctg ctcgggtgga tgttcgacgg cgcggagatg
     3961 ggcatcttct cgatggtcgg acgacccgcg gtgaaggacc tcttgtcggc gcagctgtcg
     4021 tcgctgagcg tggccgacca ggaaaaagta atcggcctgt ggttcggcat cattacggct
     4081 ggctttctgg tcggggcggc gaccggcggc gtgctgttcg gctggctggg cgaccggatc
     4141 ggtcgcgtgc gggcgatgac gctcagcgtg ctgacctacg cgctcttcac cggcctgtgc
     4201 ggtgtcgtcg gcgaggcctg gcaggtcggc atcctgcgat tcattgcgtc gctgggcatg
     4261 ggcggcgaat ggtcgctggg cgtggccctg gtgatggaag tctggccgaa caagtcgcgg
     4321 gcgttcatgg cagggctgat cggcgcggcc gccaacgtcg gctatctgct cgtcggcttc
     4381 gcgggcctgg gactgctcgc gctgatcgga acgttcaagg gctggatgct cggcgcggga
     4441 atgcccgacg cctgggtcga gatgctcgtc cgcaacaacg gctggcggct gatgatgctc
     4501 ctgggcacgc tgccggcgct gctcacgttc ttcattcgaa tcttcgtgcc tgaatcggag
     4561 aagtgggaaa aggaaaagca gaagggctcg gccgggcatt gggcaacgca agatttgctc
     4621 ggcattctgg tgggcatcgc cgggccggcg ttgatcgtgt atgtctgggc gacgccgcag
     4681 agcccctacc tgcgttttgc tgcgacgctg ttggg
//
LOCUS       DASRJB010000563         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2414787, whole genome shotgun sequence.
ACCESSION   DASRJB010000563 DASRJB010000000
VERSION     DASRJB010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414787"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..952)
                     /locus_tag="VFB80_14695"
     CDS             complement(113..952)
                     /locus_tag="VFB80_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HZN35074.1"
                     /translation="MRIGAVSYLNTKPLVYGLAELAPQHELVFDLPSRLADELAAGRL
                     DVALIPSIEYFQNPAYRIVSDACIGCRGPVRSVKLLSRVPLEEIRTLALDEGSRTSVA
                     LVQILLRERFGLSPQLLPFPIDARPESVAADALLMIGDRAMQPPPGEFVSQWDLGDVW
                     CRWAELPFVFAMWVAGRGSGVGSRESLDEIAALLNRARDNGVANVERIAAEEHSRFEL
                     SYEDCLTYLRDHLHFYLGPRELAGLRLFRQYAMTLAGTKPGPGELRTSDPLATAGTRF
                     GPG"
     gene            complement(1116..2993)
                     /locus_tag="VFB80_14700"
     CDS             complement(1116..2993)
                     /locus_tag="VFB80_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN35075.1"
                     /translation="MLIGPIFSRELVTAPRRPQHFVYRGVYGLALFVLMYTAFLVFYG
                     TQTSFTIGDLAKFGSILFLILAPLQLALLTFLAALRAASAVALEKDKKTILLLLMTRL
                     SNHELVLGKLLASLLDVFVMLATALPIFMLLTLFGGVSPAQVVRVFAVTLATILAAGS
                     LGSTVALARDKTFQSLAMTALILVIWVAGFEALALAGSGPLATFAAAASPVRAILSAA
                     HPFRDTLGLWAIDTWFVLVALAASALLNALAIARLRVWNPGRELLVKKDEEEEKDQRT
                     EEPGEKNQRTKEPKNQMKNSAFGSLVLGSSEFDPEAARQTHVDARVRAVGQKSRQVWD
                     NPVLWREMCTWAYGRKVLIIKVVYVLLFAMALAAIVWSLRTGAAYARASETILPAAAL
                     PLAPFFLVSLVIVNALAVTSITNERDGGSLDLLLVTDLSPKEFLFGKMGGVAYVTWEM
                     LAGPLVLSLVLFFAGALTLENLLYLILGLAVMDLFVITLGIHCGLNYESSRTAIGVSL
                     GSVFFLFLGVVTLLMFMISFSSSYQIQLTPFLAFVVGGSVGLFVTLGNRNSSSAIGWA
                     ALLLPFATFFAITNFLVDHIAAAFLVSSATYGFTTAAMLVPALYEFDVAMGRSKVPGE
                     E"
BASE COUNT          716 a         1127 c         1075 g          544 t
ORIGIN      
        1 cgcggcccgg ccgatcgcgg ccaggtcgtt cgattccagc agcgccaatc cctcctcgcg
       61 cgagaggcgc tcgccggctg tcgctttgtc gaggatcgat tcgatcatgt tcttagccgg
      121 gaccaaacct ggtcccggcg gtcgccaaag gatcgctcgt tcgcagttct ccgggaccag
      181 gtttggtccc ggctaatgtc attgcatatt gacggaacag ccgcagtcca gccagctcgc
      241 gcgggccgag atagaagtgc aagtggtctc gtaagtacgt caggcaatcc tcatacgata
      301 attcaaaccg cgagtgctcc tccgccgcaa tccgctcgac attcgccact ccattgtcgc
      361 gggcgcgatt gagcagcgcg gcaatctcgt ccagcgactc ccgactcccg acccccgacc
      421 cccggccggc cacccacatc gcaaacacaa aaggcagctc cgcccagcgg caccagacat
      481 cgcccaggtc ccactggctg acgaactctc cgggcggcgg ctgcatcgcg cggtcgccga
      541 tcatgagcag cgcgtcggcg gcgaccgatt cgggcctggc gtcgatcgga aacggcagca
      601 gctgcggcga gaggccgaac cgctcgcgga gcaggatctg caccagggcc acgctcgtcc
      661 gcgagccctc gtcgagcgcc agcgtgcgaa tctcctccag cggcacgcgg ctgagcagct
      721 tcacgctccg caccggcccg cggcagccga tgcaggcgtc ggacacgatt ctgtaggccg
      781 gattctggaa atactcgatc gacggaatca gggccacatc caggcggccg gcggccaatt
      841 catcggccag ccggctgggc aggtcgaaga ccagctcatg ctgcggcgcg agctcggcca
      901 gtccatagac cagcggtttg gtgttcaggt atgaaaccgc gccgattcgc attcaacgct
      961 ccgctttgct caagtccgcg attctagtgg cccaccttca cgctgacaga gggataaccg
     1021 caggtcgggg cagcattcaa catccaggcc acgccttccg agcgaattct ctccggtgaa
     1081 tacgaccata agtgctttcc gcgtaatgac ttgcatcact cttcgcccgg gaccttgctg
     1141 cggcccatgg cgacgtcgaa ctcgtagagg gcggggacga gcatggcggc ggtcgtgaag
     1201 ccgtaggtgg cggacgagac caggaacgcg gcggcgatgt gatcgaccag gaagttggtg
     1261 atggcgaaga aggtggcgaa gggcaagagc agggccgccc agccgatggc ggaggaggag
     1321 ttgcggttgc cgagcgtcac gaacaagccg acgctgccgc cgacgacaaa agccaggaac
     1381 ggcgtgagct gaatctggta ggagctgcta aagctgatca tgaacatcag cagcgtcacg
     1441 acgcccagga acagaaagaa aacgctgccc aggctgacgc cgatggcggt ccgcgagctt
     1501 tcgtagttca ggccgcagtg gatgcccagc gtgatgacga acaggtccat cactgccagt
     1561 cccaggatca ggtagagcag gttctccagc gtcagggccc cggcgaagaa cagcacgagc
     1621 gagagaacca gcggcccggc cagcatctcc cacgtgacgt aggccacgcc gcccatcttg
     1681 ccgaacagga attccttcgg cgagaggtca gtgaccaaga gcaagtcgag tgagccgccg
     1741 tcgcgctcgt tggtgatcga cgtgacggcc agcgcgttga cgatcaccag gctcaccagg
     1801 aagaacggcg ccagcggcaa ggccgcggcg ggcaggatcg tctcgctggc ccgcgcatag
     1861 gccgcgccgg ttcgcagcga ccagacgatg gcggccaaag ccatcgcgaa cagcagcaca
     1921 tacacgacct tgatgatcag caccttgcgg ccgtaggccc aggtgcacat ttcccgccac
     1981 aacaccgggt tgtcccagac ctgccggctc ttctgaccca cggcacgcac gcgggcatcg
     2041 acgtgggtct gccgagccgc ctccggatca aattccgaag aaccaagaac caaagaacca
     2101 aaggcggaat tcttcatttg gttcttcggt tctttggttc tttggttctt ttcccctggt
     2161 tcttcggttc tttggtcctt ctcctcctct tcgtcctttt tgaccagaag ctcgcggccc
     2221 gggttccaca cacgcagccg ggcgattgcc aaggcgttga gcagcgcgct tgcggccagc
     2281 gcaaccagca cgaaccaggt gtcgatcgcc cacaggccga gcgtgtcgcg gaagggatgc
     2341 gccgcgctca agatggcccg cacggggctg gccgcggcgg cgaacgtcgc cagcgggccg
     2401 ctgccggcga gggccagcgc ctcgaagcca gcgacccaga tcaccaggat cagcgccgtc
     2461 atcgccagcg attgaaacgt cttgtcgcgg gccagggcca ccgtcgagcc gaggctgccg
     2521 gcagcgagaa tcgtcgccag cgtcacggcg aagacgcgca cgacctgcgc cggcgagacg
     2581 cccccgaaca gcgtgagcag catgaagatc ggcagcgcgg tcgccagcat cacgaaaacg
     2641 tcgagcaggc tcgccagcaa cttgcccagc accagctcgt ggttcgacag ccgcgtcatc
     2701 aagagcagca ggatcgtctt cttgtctttc tcaagggcca ccgcgctggc cgcgcgcagg
     2761 gcggccaaaa acgtcagcag ggccaattgc agcggggcga gaatcaggaa caggattgag
     2821 ccgaacttgg ccaggtcgcc gatcgtgaac gacgtctgcg tgccgtagaa cacgaggaac
     2881 gccgtgtaca tcagcacgaa cagcgccagg ccatacacgc cgcgatagac aaaatgctgc
     2941 ggccgccgcg gggcggtgac gagctcgcgg ctgaatatcg gaccgatcag caagggaacc
     3001 tcaaagcggg caaatctcga cggcgctccg cctggcgact agattcaacc ggagcagcgg
     3061 attctaaccg tcagcaaccg ggcgcggcag aaccgagatc gcccggcgct gttggaatta
     3121 cgctgccccc gcgtcgtcag ttcctaccga aggccaagcc gggctggaag gcaacggccc
     3181 tcccgatcaa cgcaaagagg caaaggtgca aaggcgcaga gagggccact tctaacctct
     3241 cttggcacct ctgcccttcg cgcctttgtg tttattggaa tcgcgcagga ctaggcataa
     3301 gtatctgtcg ccaaacaact taggaacgat tctcccggcc gaatcccatt ttttcaagat
     3361 tccgaccaaa tatccccccg ccacagcgca tctgaactgt gagcgtccgg agccgagcgg
     3421 tcggccagga gcttgtgtgt ccgcggaaga tcgccagaac ga
//
LOCUS       DASRJB010000564        11275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2982049, whole genome shotgun sequence.
ACCESSION   DASRJB010000564 DASRJB010000000
VERSION     DASRJB010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982049"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFB80_14705"
     CDS             <1..585
                     /locus_tag="VFB80_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZN35076.1"
                     /translation="AMAALDAMDKTAGVRLLQTESNDFYGVVLKVTGAQANVAAAIAA
                     GKATAEQMGGKPVATILNRPDGRAWPALESKVEVSPLIQQPVVKLPNYEVVGKREGIT
                     VADNISALGFIETQGFTAVFEAIDCACKAASVEVVGKEKLGGGYITVVIKGDVAAVKA
                     AIEAGTTKVNGLGKLIASHVIPRPSAAVLSLLPK"
     gene            complement(605..1519)
                     /locus_tag="VFB80_14710"
     CDS             complement(605..1519)
                     /locus_tag="VFB80_14710"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZN35077.1"
                     /translation="MPRFAKWLIDAAPDAPVDQVARKALTTRLRAVAWYLDAAIGGED
                     ESEAIHQLRIWTRRSDAALRLFAPALADRPARRLRKILRKLRRKAGAVRDCEIQRQQI
                     QDSADRPPRHICSQLDKQHRHARQKLKSLRRRVHKADRLQRRSNDVLEAIAWPKRHSS
                     REAPPFAPWCRQQLAALGQLFFESAAIQPADDRQLHELRKAGKRLRYALELSPAALPP
                     RVHRRLYDELSRLQDRLGAVCDRLVSVEQFRDWESAAHKRKHREQLAMLLSQEDQRLA
                     AARQQFHRWWSGARRARLKRQWEAATAA"
     gene            1849..2478
                     /locus_tag="VFB80_14715"
     CDS             1849..2478
                     /locus_tag="VFB80_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN35078.1"
                     /translation="MYSQRSLTQTLRPGRRGFSLVEALVALTIMALAGSVLLLSVESS
                     LETTTDAVDRTIADGIAQQVLDQALTEKYTESAGSGLTLLEILGPLGPAADELLGVGT
                     SLFDDVDDYAGYIAQPVKDEYGEVLGTGDDSGGLRSTTFRIPSGFFQQWRYRVKVYYV
                     DASDHRTESSSATAYRAIEVFVEKLGEGGRVIPLASRKRIVTYLPPPAS"
     gene            2489..3307
                     /locus_tag="VFB80_14720"
     CDS             2489..3307
                     /locus_tag="VFB80_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35079.1"
                     /translation="MRVKQIATAGRRALTLAELLVASAIMVLIAAGLATLSATVHSAN
                     TYAHGRIVCAQHARVALDRIEHAVENAAVSEQFPACLVVTEQVGSEELPHTLVVWSPT
                     ATAANPSGLPLVKELAIYAPDPAKPSRLTEFRASNNANVVPAPSNVTAWRTLVESLKT
                     SDTTEKIVLTDRLRTAPLSGEWTSNLTAAQLRGVVRFHRLVAPSEQQWAEYKAGTRAW
                     NALAWPLDSYRTTSGTRAIACQTELQVVAGHMASAASTAVPFFGSAMRTHELAR"
     gene            3323..4891
                     /locus_tag="VFB80_14725"
     CDS             3323..4891
                     /locus_tag="VFB80_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35080.1"
                     /translation="MNHSQRLTRRGMAILLVLGVLAITLAVCYATLRGQGATAQLARN
                     NSRALQAREAAHSGLAAALRKMSEAGWAGVNVPLNSYVTPDSWYEVTFSTGDPTLTAG
                     HPKFGEYPYRVTINSTGLASDPSDPTIRAIHKSRCVVQLIRKAIASEPPAWPKLTSST
                     VYQWGNRNTYLQEPVRINGRTTILGRIYLSTEYPLTTPSKERYLGDLKQMSSANRGDH
                     RPVESPLYVAVARQDLPTMTLLNVNLGLLTIDTLDSTANPLTHPGAVTSYQLYPGGRS
                     YAPPVLQLNYGSAVQDLTLAPDPVANPLGVFRSSGTLTINNNVSITGTLISETTMSEI
                     YVAGTNVVVQAANLPKLYGSNTVVQLPATIARDSIFINSAAAAQWKGFTMVWDDFDMK
                     KGAATTQFTMVGNLATAGLMLRGREPWTMTSTAWNNDYNDYMGSGGLLPALLNALFNT
                     IRAALGIPSGEPVYFPEYMQHVRGFTIQPTLTFQPDLSGVQPHWQDWSQPVFQKGPGD
                     EGLCWDVIRWEDNL"
     gene            4926..5183
                     /locus_tag="VFB80_14730"
     CDS             4926..5183
                     /locus_tag="VFB80_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35081.1"
                     /translation="MAMSTVVRSRWTWLVAANLAALGMLGFYSQLGAAPPAGQPPFAN
                     PIDQRLDMIRELREIKELLKEQNDLLRGGADKTNAIRPAGR"
     gene            5158..5784
                     /locus_tag="VFB80_14735"
     CDS             5158..5784
                     /locus_tag="VFB80_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN35082.1"
                     /translation="MPFDPRDASTSIALDRSARRGLTLIELLLTIAVLGILAAALIPQ
                     LTSDLPERLDAAAQVVAADLDYTRALAVAHNSTYQVVFQPAQNRYFLRHSGANSLLNA
                     LPRSPFRQNSDAPDQQTTRLADLPLPEPGVRLVGAVVMQGGGQAAGNVEFTPLGGTTT
                     KYETVVWLACGQGGSQRYISVHINPITGLSEIGPLQGALPPAVAAIQF"
     gene            5805..6863
                     /gene="pilM"
                     /locus_tag="VFB80_14740"
     CDS             5805..6863
                     /gene="pilM"
                     /locus_tag="VFB80_14740"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZN35083.1"
                     /translation="MVRWLPKRRFSPIGVDIGARALKLVQFSGDHSRLIDAARVELPA
                     LSDQATPEQQANRIVEGLKRGLDDRDFRGREAVLCLNDKQLCLQSVRVPKQTGASLDQ
                     HVAQEAVSRVPFGIDEAEIRYLEAADVRQGDQTLREVIVFAVQRTVLQQALDTVERAK
                     LHPVAVDVEPAALVRSYTLQFRREDDKLARALLVHIGYTRTAALIVQADELLFVKYID
                     IGGQSFDQAVARHLKMALPDAVSLRKHNGDRRAELQDPEVAKSVAAATRPIVERLASE
                     LAMCVRYHSVTFRGQPIVRMVMSGGEATPQLVEAVGKQLDLKCELSDPFRGLPQIPNV
                     GRKGQWDVAAGLAMRELN"
     gene            6933..7706
                     /locus_tag="VFB80_14745"
     CDS             6933..7706
                     /locus_tag="VFB80_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35084.1"
                     /translation="MKNIDFLPDIYRQRAALRKARIWWCAVVVLFGGAISSAFTAQVW
                     LRVSLKRQLAALEESFLSSQTQVRELANLQAQIAKSAHEASLFTYLQHPWPRTQLLAQ
                     VVRPLPKSIRLTQIHVSDEELPRDAPHAAPLHRGPETEPAAKPQPAEDDLAKLQQEMD
                     FKATIVQLQGFTPEAADLHEYVALLNGSSLIAAAQIKSLESDSTHDPPRTRFTLRLIV
                     HPGYGQRGSEGPPAPPTDGRGQSWTRLRGNGNAPSGGGG"
     gene            7703..8269
                     /gene="pilO"
                     /locus_tag="VFB80_14750"
     CDS             7703..8269
                     /gene="pilO"
                     /locus_tag="VFB80_14750"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZN35085.1"
                     /translation="MKKLIASPRSGKPRTWIITALLAALAVGYVVFVFIPCQRSISLL
                     QSQVQERQQQILHAQSLARPLVQARERLAQTREVSRQWRDEAPRHTELISHLANLTKQ
                     AEAAGVKMDRLDPLPAVELRAVAQQNVTLQFQGAFRETFDLLRRMESLRGTIWMRDLR
                     LTATDETNNQLRGELTLTIFVDRAVYPD"
     gene            8420..9013
                     /locus_tag="VFB80_14755"
     CDS             8420..9013
                     /locus_tag="VFB80_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35086.1"
                     /translation="MALVALYFWAPLVWGWLSQGERKGAGAGAAELILKDDPEEPAAQ
                     TKRRASKFRWEKVRQAIAQDGRMASAGFDNQWTDPFAGIAAPRTAAAAVPTASGAQAL
                     IAGADLDPAQAGLVLSSVMIGNQRRTATINGDTYREGSTIRIQGEAGRPGAVEFRLVR
                     VTGRGVELERRGKTYVLLFAQPGLAKGDQIERTKQSD"
     gene            9035..11197
                     /locus_tag="VFB80_14760"
     CDS             9035..11197
                     /locus_tag="VFB80_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HZN35087.1"
                     /translation="MPTLLRITLIAVSLALGIAVALGLALREPARPVARQARAAAPAA
                     AEAPPVAPAATPAPVSAPYRDPVAARINELHESMLEIEQSSQRRQASVMRAVSLLQDQ
                     LAEAQQAPPPAAPAAAPPLAAAPAAAIPPADPLAGGDRIEDEGDGKLSINAQNSDIRV
                     VLEQLSQQAGLNIMAGRGVTGSVTANLSGVSVETALAAILKTTGFVARREGNIIYVGT
                     PAELLQMDQSQDEIRTRVYRPNYVKAADLQQLFTSLLTPVIGKITVSSPSEIDIPQDQ
                     NKTGGNGFAGTDVVIVRDYASVIVQIDQIFEEVDVKPRQVAIEAMILSVSLSDTYKFG
                     VNFEALRDNANARLISGSPPANLANISVADGGLKFGFMDASLTLFIEALETIGDTNVI
                     ASPRLTCLDKQRAEIQIGEELGYVSTTVTESAATQTVNFLDVGTLLRIRPFIGNDGLI
                     RLEVHPELSTGSVEVQQGLSLPQKQVTQVTTNVLCPDGCTVVIGGLIREDLTTVTNQI
                     PLLGSLPWLGPVFRQKTETIDRNEIIVLITPRIVSEPFMCQEGQKLGHEFAQRQDVYF
                     DKMSPLGRRNIGQHHLRMARAAFSAGDMLTAMKQVNLAIHYDPQSREAVALRDQIVAA
                     GGFENESIHEYLHRGLAPLTGRHIDYSRRGFPWKDPPEFGGPLEVTASDDYGQVGPTR
                     DILTPPPPAGALRLPQELLPAQPLPPPRLSPPPGGPER"
BASE COUNT         2059 a         3736 c         3670 g         1810 t
ORIGIN      
        1 gcgatggcgg cgctcgacgc gatggataag accgccggcg tgcggctgct ccagaccgag
       61 tcgaacgatt tctacggcgt ggtgctgaag gtcacaggcg ctcaggcgaa tgtcgcggcc
      121 gccatcgcgg ccggcaaagc aaccgcggag cagatgggcg gcaaacccgt cgcgaccatt
      181 ttaaatcggc ccgacggccg cgcgtggccg gccctcgaaa gcaaggtcga agtcagcccg
      241 ctcatccagc agcccgtcgt caaactcccg aattacgaag tcgtcggcaa acgcgaggga
      301 ataaccgtgg cagacaacat ctccgccttg ggattcatcg aaacgcaagg cttcaccgcc
      361 gtgttcgagg ccatcgactg cgcctgcaaa gcggccagcg tcgaggtcgt cggcaaggaa
      421 aaactgggtg gcggatacat caccgtcgtc atcaaggggg acgtcgccgc cgtgaaagcc
      481 gctatcgaag ccggcacgac gaaggtgaac gggctgggca agctgattgc cagccacgtg
      541 attccgcgcc cctcggcggc ggtcctgagc ttgcttccga aataactgat ggtgggagcg
      601 gcaatcaagc tgccgtagcc gcttcccact gccgcttgag ccgcgcgcgg cgggcacccg
      661 accaccagcg gtggaattgt tggcgggcgg cggctagccg ctggtcttcc tgcgatagca
      721 gcatggcaag ttgctcgcgg tgcttccgct tgtgggcggc cgattcccaa tcgcggaatt
      781 gctcaacgga aaccagccgg tcgcagacgg cgcccaaacg gtcctgcaag cggctcagct
      841 cgtcatacag ccggcggtga acgcgcggcg gaagggcggc gggcgacaat tccagcgcgt
      901 accggagccg cttgccggcc ttccgcaatt cgtggagttg ccggtcgtcg gccggttgga
      961 tcgcggccga ctcgaagaac aactgaccca gcgcggccaa ctgctggcgg caccagggcg
     1021 caaaaggcgg cgcttcgcgg ctggaatgcc gtttgggcca ggcgatcgcc tccagcacgt
     1081 cattcgagcg gcgctgcagg cggtctgcct tgtgcacgcg ccggcgaagc gatttcagct
     1141 tttgccgggc gtggcgatgc tgtttatcca actgcgagca aatgtggcgc ggcggccgat
     1201 cagcagagtc ctgaatctgc tgccgctgga tttcgcaatc gcggacggcg ccggccttgc
     1261 gccgcagctt gcgcaagatt tttcgcagtc gccgggccgg tcgatcggct agcgccgggg
     1321 caaacagccg cagggctgca tccgagcggc gggtccagat gcgcagctga tggatcgcct
     1381 cggactcgtc ctcgccgccg atcgcggcat ccagatacca ggctacggct cgcagcctcg
     1441 tcgtgagggc tttgcgcgca acctgatcga cgggagcgtc cggggcggcg tcgatcagcc
     1501 acttcgcaaa gcgaggcata cccaaccgca gatttggagc cgctgctcgg ctgagaatcg
     1561 actggcggct tcctgcacac aaggaggcgg gctcgtcccc gcaaaccatt tgattgtaat
     1621 ccggcctgcg ggcgacgtgg gcgaaaggaa gcctgcgtga tgaaagaatg ccgcataatc
     1681 gctggatttg acaaaatccg gccgcgacct agggtttagt tgcaaccgcg cctcggggag
     1741 gggcgctggg tgcattgggc acggcgggga tagctgggag ctagagggga ccccgccgtg
     1801 ctttttcttt ccccgcttga ttgccagcgg agcggcccac ctttcgccat gtactcccaa
     1861 cgatcgttga cgcaaaccct tcgcccgggg cgacgcgggt tttcgctcgt cgaggcgctg
     1921 gtcgccctga ccatcatggc gctggccggg tcggtgcttc tgttgtcggt tgaaagctcg
     1981 ctcgagacaa cgaccgacgc cgtcgatcgc acgatcgctg acgggattgc ccagcaggtg
     2041 ctcgaccagg cgctgaccga gaaatacacc gagtcggcag gtagcggcct cacgctcctt
     2101 gaaatactcg gccccctcgg cccggccgca gatgagttgc tgggcgtcgg cacttcgctg
     2161 ttcgacgacg tggacgacta cgccggctac atcgcgcagc ccgtcaaaga cgagtatggc
     2221 gaggtgctgg gcacgggcga cgactcgggc ggcctgcggt ccacgacctt tcgcattccc
     2281 agcggattct tccagcagtg gcgctatcgg gtgaaggtgt actacgtgga cgccagcgac
     2341 catcgcacgg agtccagcag cgccacggcc tatcgggcga tcgaagtatt tgtcgaaaag
     2401 ctgggcgaag gcggccgagt cattccgctt gccagccgca aacgcatcgt tacctacctt
     2461 cctccgccag cgagctaagg agcgagccat gcgcgtcaaa caaatcgcga ccgctggccg
     2521 ccgcgccctg acgctggcgg aactgctggt ggcctcggcg atcatggtgt tgattgctgc
     2581 cggtttggcg acgctgtccg cgaccgtcca ctccgccaac acctacgcac acgggcggat
     2641 tgtctgcgcc cagcatgccc gtgtggcgct cgaccggatc gagcacgccg tcgaaaacgc
     2701 cgccgtcagc gagcagtttc ccgcctgcct ggtcgtgacc gagcaagtcg gctcggaaga
     2761 gctgccgcat acgctcgtcg tgtggtcgcc gaccgcaacg gctgccaatc ccagcggctt
     2821 gccgctggta aaggagctgg ccatctatgc tcccgatccg gcgaagccct cgcgcctgac
     2881 tgagtttcga gcctccaaca acgccaatgt cgtcccggcg ccgagcaacg tgactgcgtg
     2941 gcggaccctc gtcgagagcc tgaaaaccag cgacaccacg gaaaagatcg tcctcaccga
     3001 ccggctgcga acggccccgc tcagcggcga gtggacttcc aacctgacgg cggcccaatt
     3061 gcgcggcgtc gtgcgatttc atcggttggt cgcgcccagc gagcagcagt gggcggagta
     3121 caaagccggc acgcgggcgt ggaacgcctt ggcgtggccg ctggacagct accggacgac
     3181 gagcggcacg cgggccatcg cctgccagac cgagctgcaa gtcgtcgcgg gccacatggc
     3241 ctcggccgcc tccacggcgg tgccattctt cggatcggca atgcggaccc atgagctggc
     3301 gcgttaacgg acggaggccg cgatgaatca ctcccaacga ctgacccgcc gtggcatggc
     3361 gattttgctc gtgctcggcg tgctggccat cacgctggca gtatgttacg cgacgctgcg
     3421 cggccaaggg gccacggccc agctggcccg caacaacagc cgcgcgctac aggcccgcga
     3481 agcggcgcat tcgggcctgg cggcggcgct gcgcaagatg agcgaagccg gttgggccgg
     3541 cgtcaacgtg ccgctcaatt cgtatgtgac gcccgactcc tggtacgaag tcacgttctc
     3601 gacgggcgat ccgacgctga ctgccggcca ccccaaattc ggcgagtacc cctaccgcgt
     3661 cacgatcaac tccaccgggc tcgcctccga tccttcggac ccgacgatcc gggccatcca
     3721 caagagccgc tgtgtggtgc agctcatccg caaggcgatc gccagcgagc cgccggcctg
     3781 gcccaagctg acgagttcca ccgtgtatca atggggaaac cgcaatacct atttgcagga
     3841 gcccgtgcgg atcaacggcc gcaccaccat tctcggccga atctacctca gcaccgagta
     3901 cccgctgacg acaccctcca aggaacgcta cctcggcgat ctcaaacaga tgtctagcgc
     3961 caaccgcggc gatcaccggc ctgtcgaaag cccgctttac gtggcagtcg cccggcagga
     4021 cctgcccacg atgaccttgc tcaacgtcaa tctcgggctc ctgacgatcg acacactcga
     4081 cagcaccgcc aacccgctta cgcatcccgg cgccgtgacg agctaccaac tctatcccgg
     4141 cggaaggagc tacgcaccgc cggtgctcca attgaactac ggcagcgcgg tgcaagactt
     4201 gacgctggca ccagatccag ttgcaaaccc gctcggagtc ttccgcagca gcggcacgct
     4261 gacgatcaac aacaacgtca gcatcaccgg cacgctgatc tccgagacca cgatgtccga
     4321 gatttatgtc gcgggtacga acgtcgtggt acaggcggcc aacctgccca agctgtatgg
     4381 ctcgaatacc gtcgttcaac tgcccgcgac gatcgcccgc gatagcatct tcatcaactc
     4441 ggcggccgcc gcccagtgga agggcttcac gatggtctgg gacgacttcg acatgaaaaa
     4501 aggcgctgcg accacgcagt tcacgatggt ggggaacctc gccacggcag ggctgatgct
     4561 ccgcgggcgc gagccgtgga cgatgacctc gacggcctgg aacaacgact acaacgacta
     4621 catgggctcc ggcggcctcc tgcccgctct gctcaacgct ttgttcaaca cgattcgcgc
     4681 ggccctgggg atcccctccg gggaaccggt ctattttccc gaatacatgc aacacgttcg
     4741 tggcttcacg atccaaccga ccttgacgtt tcagcccgat ttgagtggag ttcagccgca
     4801 ttggcaggac tggtcgcagc ccgtgttcca aaaggggcct ggcgacgagg ggctctgctg
     4861 ggacgtgatc cgctgggaag acaacctgta acgatgctat cagtcgtcag cctggaaggg
     4921 gttttatggc tatgagcacg gtcgttcgct cgcgctggac gtggctcgtg gcggccaatt
     4981 tggccgcgct gggcatgcta ggtttttaca gccagcttgg cgcggccccg ccggcgggcc
     5041 agccgccgtt cgctaacccg atcgaccagc gcctggacat gatccgggag ctgcgcgaga
     5101 tcaaagagct gctgaaggag cagaacgacc tgctccgcgg cggcgccgac aaaaccaatg
     5161 ccattcgacc cgcgggacgc tagcacctcg atcgccctcg atcgcagtgc gcgccgcgga
     5221 ttgacgctca tcgagctcct gctcacgatc gcggtgctgg gaattctcgc cgccgcgctc
     5281 atcccgcagc tcacgagcga cctgcccgag cggctggacg ccgcggccca ggtcgtcgcg
     5341 gccgacctcg attacacccg cgccctggcc gtcgcgcaca actcgacgta tcaggttgtc
     5401 tttcagcctg ctcagaatcg ctacttcctg cggcacagcg gggccaactc gctgctcaat
     5461 gcgctacccc gctcgccttt caggcagaac agcgacgcgc ccgatcagca gacaacgcga
     5521 ctggctgatc tgccgctccc cgagcccggg gtgcgactgg tgggtgcagt cgtgatgcaa
     5581 ggcggggggc aagcggccgg caacgtcgaa ttcactcccc tgggcggcac gactaccaag
     5641 tatgaaacgg tcgtgtggct ggcctgcggt cagggcggat cgcagcgcta catttcggtc
     5701 cacatcaatc cgatcaccgg gttgtccgaa atcggaccgc tccaaggcgc gctgcctccg
     5761 gccgtggccg cgattcagtt ctagactctc gcggaattcg ccccatggtt cgctggctcc
     5821 ccaaacgacg cttcagcccc atcggcgtgg acatcggcgc tcgcgcgctc aagctggtgc
     5881 agttcagcgg cgaccactcg cgattgatcg acgcggcgcg ggtcgagctg ccggccttga
     5941 gcgaccaggc gacgcccgag cagcaggcga atcggatcgt cgagggacta aagcgcggct
     6001 tggacgatcg cgacttccgg ggccgcgagg cggtgctctg tctgaacgac aagcagctct
     6061 gcctgcagag cgtgcgcgtg cccaagcaaa cgggagcgtc gctcgaccag cacgtggccc
     6121 aggaggcggt cagccgcgtg cccttcggca tcgacgaagc ggaaatccgc tatctcgagg
     6181 cggccgacgt gcgccaggga gaccagacgc tccgggaggt gatcgtcttc gccgtccagc
     6241 ggacggtcct gcagcaggcg ctggatacgg ttgagcgcgc taagttgcac ccggtggccg
     6301 tcgatgtgga gccggcggca ctggtccgca gctacacgct gcagttccgc cgcgaagacg
     6361 acaagctggc gcgggccttg ctcgtgcaca tcggctacac gcgcacggca gcgctgatcg
     6421 tgcaggccga cgagctgctg ttcgtgaagt acatcgatat cggcggccag agcttcgatc
     6481 aggctgtggc ccggcacctg aagatggcct tgcccgatgc ggtctcgctt cgcaagcaca
     6541 acggcgaccg gcgggccgag ctgcaggacc cggaagtggc caagagcgtc gccgccgcca
     6601 cgcggccgat cgtggagcgc ctggccagcg agttggccat gtgcgtccgc taccacagcg
     6661 tgacgtttcg cggccagccg atcgtgcgga tggtgatgag cggcggcgag gcgacgcccc
     6721 agctggtcga ggcggtcggc aagcagctcg acctgaaatg cgagctcagc gatcccttcc
     6781 gtgggttgcc gcagattccc aatgtcggcc gcaagggcca atgggacgtt gcggcgggac
     6841 tggcgatgcg agaactcaac taattcaagt tcaaggttca aggttacatg ttaccggctc
     6901 tgcaactttg aaccttgaac cctgcctgaa acatgaagaa catcgacttc ctgcccgaca
     6961 tctaccgtca gcgagcggcg ctgcgcaagg cccgcatctg gtggtgcgcg gtggtcgtat
     7021 tgttcggcgg cgcgatctca tcggcgttca cggcccaggt ctggctccga gtcagcctga
     7081 agcgccagct ggcggcgttg gaagaatcgt ttttgtcctc gcagacgcag gtccgggagc
     7141 tggccaacct gcaggcccag atcgccaaat ccgcccacga ggcgagcctg ttcacctacc
     7201 tgcagcaccc ctggccgcgg acgcagctgc tggcccaggt cgtgcggccg ctgcccaagt
     7261 cgatccgcct gacgcagatt cacgtcagcg atgaggagct gccgcgcgac gcgccgcatg
     7321 ccgccccgct ccatcgtggc ccggagacag agcccgccgc gaagcctcag ccggctgaag
     7381 acgatctcgc gaagttgcag caggagatgg acttcaaggc tacgatcgtt cagcttcaag
     7441 ggttcacgcc cgaagccgcc gatttgcacg agtacgtggc actcctgaac gggtcttcgc
     7501 tgattgcggc ggcccagatc aagtcgctcg aatccgactc gacccacgac ccgccgcgaa
     7561 cccgattcac gctgcggctg atcgtgcatc cgggctacgg gcagcgcggc agcgagggcc
     7621 cgccggcccc gcccaccgat ggccgggggc aatcgtggac gcgcctccgc ggaaatggca
     7681 acgctccaag cggaggcggc ggatgaaaaa gctcattgcc tcgccccgct cgggcaaacc
     7741 gcggacgtgg atcatcacgg cgctgctggc agcgctggcc gtcgggtacg tggtgtttgt
     7801 cttcattccg tgccagcggt cgatcagcct gctccagtcg caggtgcagg agcggcagca
     7861 gcagatcctg catgcccaga gcctggcccg gccgctggtg caggcccgcg agcggctggc
     7921 ccagacccgc gaggtcagcc gacaatggcg ggatgaagcg ccgcggcaca ccgagctgat
     7981 cagccacctg gcaaacctga cgaagcaggc cgaggcggcc ggcgtgaaga tggaccggct
     8041 cgatccactg ccggccgtcg agctgcgggc cgtcgcgcag caaaacgtta cgctccagtt
     8101 tcagggcgca ttccgcgaga cgttcgacct gctgcggcgg atggaatcgc tgcggggaac
     8161 gatctggatg cgcgacctgc ggctgacggc caccgacgaa acgaataacc aattgcgggg
     8221 cgaattgact ttgacgattt ttgttgaccg tgccgtttat cccgattagg cgggccggtg
     8281 cgggccgata actgaggagc cggactagcc tgcgccaaac gtgctggcat ctggtgcgac
     8341 cgtgaaactg gacaaattag cgaaacgact cggccgcgac ctagcggcga atcccaaaaa
     8401 ggcagccgcc ctgggcgtta tggctctggt ggctctgtac ttctgggcgc cgctggtctg
     8461 gggctggctt tcgcaggggg aacgaaaggg ggccggtgcc ggagccgctg agctaatcct
     8521 gaaggatgat cccgaggagc cagcggcaca aaccaagcgc cgcgcgagca agttccgctg
     8581 ggagaaagtc cggcaggcga tcgcgcaaga cggcaggatg gcgtcggccg gtttcgacaa
     8641 ccaatggacc gatccgttcg ctggaatcgc agcgccgcga actgcggccg cggcggttcc
     8701 aaccgcgagc ggggcccagg cgctaattgc cggcgcggac ctcgatccgg cgcaggcggg
     8761 cctggtgctg agcagcgtga tgatcggcaa ccagcggcgg acggcaacga tcaacggcga
     8821 cacctatcgc gaaggaagca cgattcgaat ccagggcgag gccggacggc ccggagcggt
     8881 ggagtttcgc ctggtgcgag tcaccggtcg cggcgtggaa ctggaacgca gaggaaagac
     8941 ctacgtgctg ctgttcgccc agccgggatt ggccaagggc gaccagattg aacggacgaa
     9001 acagagcgat tgaggacctg gtcacggacg acctatgcca acgctgcttc gcatcacgct
     9061 gatcgccgtc tcgctggcgc tgggcattgc cgtggcgctg ggcctggccc tgcgcgagcc
     9121 cgccaggcca gtggcgaggc aggccagggc cgcggcgccg gcggcggctg aagcgcctcc
     9181 tgtagcgccg gcggcgacgc cggccccggt ttccgcgccg tatcgcgatc cggtggcggc
     9241 gcgaatcaat gagctgcacg aatcgatgct cgagattgag cagtcgtctc agcggcggca
     9301 ggcctcggta atgcgggcag tctcgctgct gcaagatcaa ctggccgaag ctcagcaagc
     9361 gccgcctccg gctgcgccgg cagccgcacc accgctggcc gcggcgccgg cggccgccat
     9421 tccacccgcc gatccgctgg ccggcggcga ccggatcgag gacgaaggtg acggcaaact
     9481 gagcattaac gcgcagaatt cggacattcg cgtggtcctg gagcaactga gccagcaggc
     9541 gggcctgaac atcatggccg gccgcggcgt cacgggctcg gtgacggcca acctctcggg
     9601 cgtgagcgtc gaaactgcgc tggccgcgat tctgaaaacg acgggcttcg tcgcccgccg
     9661 cgaggggaac atcatctacg tgggcacgcc cgccgagctg ctccagatgg accagtcgca
     9721 ggacgaaatc cgcacgcgag tctaccggcc caattacgtc aaggccgctg acttgcagca
     9781 gctcttcacg tcgctgctca cgccggtgat cggcaagatc accgtcagca gcccgtccga
     9841 gatcgacatc cctcaagatc agaacaagac cggcggcaac ggtttcgccg gcaccgacgt
     9901 cgtgatcgtc cgcgactatg cttccgtgat cgtccagatc gatcagatct tcgaagaggt
     9961 ggacgtcaag ccgcggcaag tggcgatcga ggcgatgatc ctcagcgtga gcctcagcga
    10021 tacatacaag tttggcgtca actttgaggc gctccgcgac aacgccaacg cccgcctcat
    10081 cagcggctcg ccgccggcga acctcgccaa tatcagcgtc gccgacggcg gcctcaagtt
    10141 cggcttcatg gacgccagcc tgacgctgtt catcgaggcc ctggagacga tcggcgacac
    10201 caacgtcatc gcctcgccgc ggctgacctg tctcgacaag cagcgggccg aaattcaaat
    10261 cggcgaagag ctgggctacg tcagcaccac cgtgaccgaa agcgccgcca cgcagaccgt
    10321 caacttcctc gatgtcggca cactgctgcg gattcgcccg ttcatcggca acgatggcct
    10381 gatccgcctg gaggtccacc cggaactttc gaccggctcg gtcgaagtcc agcagggcct
    10441 gtcgctgccg cagaagcaag tcacgcaagt cacgacgaac gtgctctgcc ccgacggctg
    10501 cacggtcgtc atcggcgggc tgatccgcga ggacctgacc acggtgacga accagatccc
    10561 gctgttgggt tcgctgccct ggctggggcc ggtcttccgt cagaagacgg aaacgatcga
    10621 ccgcaacgag atcatcgtgc tgatcacacc gcggatcgtc tccgagccgt tcatgtgcca
    10681 ggaggggcag aagctgggcc acgaatttgc ccagcggcag gacgtctact tcgacaagat
    10741 gagcccgctc ggccgccgca acatcgggca gcatcacttg cggatggcgc gggccgcgtt
    10801 cagcgcgggg gacatgttga ccgccatgaa gcaggtcaac ctggcgatac actacgaccc
    10861 gcaaagccgc gaggccgtcg ccctccgcga ccagatcgtc gccgcgggcg gcttcgagaa
    10921 cgagagcatt cacgaatacc tccatcgtgg cctggcgccg ctcaccggcc ggcacatcga
    10981 ctactcccgc cgcggttttc cctggaagga tccgccggag ttcggcgggc cgctggaggt
    11041 cactgcctcg gacgactacg ggcaagtcgg ccccacgcgc gacatcctga cgccgccgcc
    11101 gcccgcaggc gctctgcgcc tgccgcagga gctgctgccc gcgcagccgc tgcctccgcc
    11161 gcgactgagc ccgcccccgg gagggcccga acgatgaatg gctgcctgcg gctggcgctg
    11221 ttggcgagta tcctgttcgg cggctgtgcc gcctggcgcg gccagcggcc ggcta
//
LOCUS       DASRJB010000565         5482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2126122, whole genome shotgun sequence.
ACCESSION   DASRJB010000565 DASRJB010000000
VERSION     DASRJB010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126122"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFB80_14765"
     CDS             <1..744
                     /locus_tag="VFB80_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN35088.1"
                     /translation="VTNKHLLPVYDKPMIYYPIECLLNAGIRDIMIVTGGEHAGDFLK
                     LLKNGKQLGVKHLEYAYQEGEGGIADALKLAEDFAEGDKICVVLGDNIIQGNIRRAAG
                     DFFTQRSGAKLLLKEVTDAQRFGVVRLGDDGRIDEIIEKPENPPSNKAVIGIYFYDAD
                     VFDVCRTLKPSRRGELEITDVNNYYLQRGDLTCEILDGWWTDAGTFESLLRASSLVAG
                     GGANHAHSTTRPPAPAKAPPPLAVELLTI"
     gene            778..1281
                     /locus_tag="VFB80_14770"
     CDS             778..1281
                     /locus_tag="VFB80_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35089.1"
                     /translation="MTSFNKIEAVPTQAGDVAAAHARALAESQPVFMPAAIFADDRGW
                     SIMNQLQGVMGKEGQINYSVTYPGVIKAWHRHQHQADFWLVLMGHIKVGIHRDDGRTW
                     RTVIGEKKPGILIIPPPLWHGAATVGHEPAGLLYYVTKAYSAQQPDEERRAYDSVAGF
                     SWSVEHK"
     gene            1278..2144
                     /gene="rfbD"
                     /locus_tag="VFB80_14775"
     CDS             1278..2144
                     /gene="rfbD"
                     /locus_tag="VFB80_14775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZN35090.1"
                     /translation="MKGGSDNPILLLGGSGMLGRAWKALLDESDCAFEAPSSEACDLR
                     SAVSVRGAVHGRHRAVINCAAATDVDGCEVRQIDAHAINGLGVGILARRCAELNVPLI
                     HYSTDYVFDGLASAPYPVQTPRRPLNIYGASKALGEQLIEEAAGPHLILRTSWLYAAW
                     GKNFVRTIARLAAERPVLRVVNDQRGRPTACEALVAASWQLLARGCRGIYHVSNEGNC
                     TWFELASEIVRLTGAACRVEPCTTAEFPRPAPRPAYSVLDLSLTTAAIGPLSHWRDSL
                     AETITRLEPAAA"
     gene            2141..3184
                     /gene="rfbB"
                     /locus_tag="VFB80_14780"
     CDS             2141..3184
                     /gene="rfbB"
                     /locus_tag="VFB80_14780"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZN35091.1"
                     /translation="MTKRLLVTGGCGFIGSNFVRFALDQRPDYQITNLDCLTYSGNPE
                     NLADLESDPRYEFVRGNILDRPLVERLLDRCDAVVHFAAESHVDRSIQDSRPFIESNV
                     MGTQTLLDAARTVWADGRGGFRGRLLHVSTDEVYGSLPLGRPDLLFTETTPITTSSPY
                     SASKAASDLLVHAYHHTFHLDVVTTRCSNNFGPYQFPEKVIPLFITNLIEGQQVPLYG
                     DGQNVRDWLHVDDHCEAILTVLERGRAGEVYNIGGNNERSNLELTHTLLELMGKGREF
                     IKYVPDRPGHDRRYAIDASKIRRELGWQPTRSAWPAALAATVRWYIENESWWRRVKSG
                     AYREYYARQYAVA"
     gene            3211..4122
                     /locus_tag="VFB80_14785"
     CDS             3211..4122
                     /locus_tag="VFB80_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN35092.1"
                     /translation="MSTLIETETADAAAAALAPVDREHAEPHLPDEHLTVIQARPGWQ
                     IVDFAEMWRYRELLCFLVWRDVKVRYKQTVLGAAWAVLQPFAQMVVFSIFFGRMAEMP
                     SSSIAYPLFAFAGLLPWTFFSNAIASAASSVVGNQNLITKVYFPRLFVPASAIGVGLV
                     DFGIAFGMLLVLMLYYGVWPSAGLLLVPLILVGLTAAALGVGTLLSALTVAYRDFRYV
                     VPFMVQLWMFATPCVFMDATMVGPRWQVVLPLNPAYGLILNFRNAALGRPLDTYSLAV
                     SLAVTAGLLILSCLYFRRVERTFADII"
     gene            4745..>5482
                     /locus_tag="VFB80_14790"
     CDS             4745..>5482
                     /locus_tag="VFB80_14790"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN35093.1"
                     /translation="MFTELIKRLIGRFGYDIRYSPTTVGECAGRYWILDIQRLLTAQS
                     PLILDIGANEGQTAREFKARWPQASVHSFEPAPETFDRLAATAKSLKGVTANNVALGA
                     KPGTAPLICHRHSVLNSILPPGRDYDTWETLGTCEVEVSTVDTYCAERGIERIDVLKT
                     DTQGFDLEVLRGSREMFVGNRVHLVFAEITFTEMYDGQARYDQVFGILADHGFSIVSL
                     YDLRYRDHRLEWCNALFVNSNWQSTHIG"
BASE COUNT          999 a         1906 c         1591 g          986 t
ORIGIN      
        1 gtgaccaaca agcatctgct gccggtctac gacaagccaa tgatctacta cccgatcgaa
       61 tgcctgctga acgcgggcat ccgggacatc atgatcgtga ccggcggcga gcacgccggc
      121 gactttttga agctgctcaa gaacggcaag cagcttggcg ttaagcacct ggagtacgcc
      181 tatcaggaag gcgaaggcgg aattgccgac gcgctcaagc ttgccgagga ttttgccgag
      241 ggcgataaga tctgcgtcgt gctcggcgac aacatcatcc agggcaacat ccgccgcgcc
      301 gccggcgact tcttcaccca acggagcggc gcgaagctcc tgctcaagga agttaccgac
      361 gcccagcgat tcggcgtcgt gcggctcggc gacgacggcc ggatcgacga gatcatcgag
      421 aagcccgaaa atcctcccag caacaaggcg gtcatcggca tctatttcta cgacgccgac
      481 gtgttcgacg tctgccgcac gctcaagccc tcgaggcgcg gcgagctgga gatcaccgac
      541 gtcaacaact actacctcca gcgcggcgac ttgacctgcg agatcctcga cggctggtgg
      601 accgacgccg gcacgttcga aagcctgctg cgggccagct cgctcgtcgc cggcggcggc
      661 gcgaatcacg cccactcgac cacccgcccg cctgctcccg cgaaagcgcc gccgccgctg
      721 gccgtcgagc tgctgaccat ctgagcggag gactccgcga ctcggtacat ctgcgtcatg
      781 acctcgttca acaaaatcga agccgttccc acccaggccg gcgatgtggc cgcggcccac
      841 gcccgcgctc tggccgaatc gcagccggta tttatgcccg cggcgatctt tgccgacgac
      901 cgcggctggt cgatcatgaa ccagctgcaa ggcgtgatgg gaaaagaagg gcagatcaat
      961 tactcggtga cctaccccgg cgtcatcaag gcctggcacc gccatcagca ccaggccgac
     1021 ttctggctcg tgctgatggg ccacatcaag gtcggcattc accgcgacga cggccgcacg
     1081 tggcggaccg tcatcggcga gaagaagccg gggatcctca tcattccgcc gccgctgtgg
     1141 cacggcgcgg cgacggtcgg ccacgaaccg gctggcctcc tgtattacgt gaccaaggcc
     1201 tatagcgccc agcagcccga cgaagagcgg cgagcgtatg acagcgtcgc cggattctcc
     1261 tggagcgtag agcacaagtg aaaggcggta gcgacaaccc gatcctgctg ctcggcggca
     1321 gcggcatgct cggccgcgcc tggaaggcgc tgctcgatga gtccgactgc gcattcgaag
     1381 caccctcctc agaggcatgc gacctccgct cagcggtgtc cgtgcgcggc gcagtccacg
     1441 gccgccatcg ggccgtcatc aactgcgccg ccgcgaccga cgtcgatggc tgcgaggtcc
     1501 ggcagatcga cgcccacgcg atcaacggcc tcggtgtcgg catcctcgcc cgccgctgtg
     1561 ccgagctgaa cgttccgctc atccattact cgaccgacta tgtttttgac gggctcgcct
     1621 ccgcgcccta tccggtccag acgccgcgcc ggccgctgaa tatctacggc gccagcaagg
     1681 cgctcggcga gcagctgatc gaggaggccg ccggcccgca tctcattctc cgcaccagct
     1741 ggctctatgc cgcctggggc aagaattttg tccgcacgat cgcccgcctc gcggccgagc
     1801 ggcccgtgct ccgcgtcgtc aacgaccagc gcggccgacc cacggcctgc gaggcgctcg
     1861 tcgcggccag ctggcaactg ttggcccgcg gctgccgcgg catctatcac gtctcgaacg
     1921 aaggcaattg cacctggttc gaactcgcct cggaaatcgt ccgccttacc ggcgcggcct
     1981 gccgcgtgga gccctgcacg accgcggagt ttccgcggcc cgcgccgcgg ccggcgtaca
     2041 gcgtcctcga cctgtccctc accaccgccg cgatcggccc gctgtcccac tggcgcgatt
     2101 cgctcgccga aaccatcacc cgactggagc ccgccgccgc atgaccaaac gtctgctcgt
     2161 caccggaggc tgcggcttca tcggttcgaa tttcgtgcgc ttcgctctgg atcagcgccc
     2221 ggactatcag attacgaacc tcgattgtct gacctattcc ggcaatccgg aaaacctcgc
     2281 cgacctcgaa agcgatcccc gctacgagtt cgttcgcggc aacatcctcg accgcccgct
     2341 cgtcgagcgg ctgctggacc gctgcgacgc cgtggtccac ttcgcggccg aaagccacgt
     2401 cgatcgctcg atccaggact cgcggccgtt catcgaatcg aacgtgatgg ggacgcagac
     2461 gctgctcgac gccgcccgca ccgtctgggc cgacggccgc ggcggctttc gcggccgcct
     2521 gctgcacgtc agcaccgacg aggtctatgg ctcgctcccg ctcgggcggc ccgacctgct
     2581 cttcaccgaa acgacgccga tcacgaccag cagcccctac agcgccagca aggccgccag
     2641 cgatctgctc gtgcatgcct accaccacac gttccacctc gacgttgtca ccactcgctg
     2701 ctcgaacaac tttggcccct accagttccc ggaaaaggtc atcccgctgt tcatcaccaa
     2761 cctgatcgaa ggccagcagg tcccgctcta tggcgacggg caaaacgtcc gcgactggct
     2821 gcacgttgac gaccactgcg aggcgatcct gaccgtgctg gagcgcggcc gcgccggcga
     2881 ggtttacaac atcggcggca acaacgaacg cagcaacctg gagctcacgc acacgctgct
     2941 ggagctgatg ggcaagggcc gcgagttcat caagtacgtt cccgaccgcc cgggccacga
     3001 ccgccgctac gccatcgacg cctccaagat ccgtcgcgag ctcggctggc agccgacgcg
     3061 ctcggcctgg ccggccgccc tggcggcgac ggtccgctgg tacatcgaaa acgaaagctg
     3121 gtggcgccgc gtcaagagcg gcgcttatcg cgaatactac gcccgccagt acgccgtggc
     3181 ctgacctcct tcctcgtacg cttcagctcc ttgagcacgc tcatcgaaac cgaaaccgcc
     3241 gacgcagcgg ccgccgccct ggcgcccgtc gatcgggagc acgccgagcc tcacctgccc
     3301 gacgagcacc tgaccgtcat ccaggcccgg cccggctggc agatcgtcga tttcgccgaa
     3361 atgtggcgct atcgcgagct gctctgtttt ctggtttggc gcgacgtgaa agtccgctac
     3421 aagcagaccg tgctcggcgc ggcctgggcc gtgctgcagc cttttgccca gatggtcgtg
     3481 ttcagcatct tcttcggccg gatggccgag atgccctcct cttcgatcgc ctacccgctg
     3541 ttcgccttcg ctggcctctt gccctggacc ttcttcagca acgccatcgc ctccgccgcc
     3601 agcagcgtcg tcggcaacca aaacctgatc accaaggtct acttcccccg cctgttcgtg
     3661 ccggccagtg cgatcggcgt gggtctggtc gatttcggca tcgccttcgg catgctgctg
     3721 gtcctgatgc tctactacgg cgtctggccg tcggccggcc tgctcctcgt tccgctgatc
     3781 ctcgtcggcc tgacggcggc ggccctcggc gtcggcacgc tcctctcggc cctgacggtg
     3841 gcctaccgcg acttccgcta cgtggtcccg ttcatggtcc agctctggat gttcgccacc
     3901 ccctgcgtct tcatggacgc cacgatggtc ggcccccgct ggcaggtcgt cctgccgctg
     3961 aacccggctt acggcctgat cctcaacttc cgcaacgccg ccctcggccg cccgctggac
     4021 acttactcgc tcgcagtctc cctggccgtc accgccggcc tcctgatcct cagctgcctc
     4081 tacttccgcc gcgtcgaacg gacctttgcc gacatcatct agtccgaggc gattcggtac
     4141 gtcagcacgc ctttcggagg gtcagccgtc aatccggaaa acacgcctcc ccgacattag
     4201 cattagacgc aagtatatac ccatacatga cttatgacga catttggccg tgacaaccac
     4261 attagcgttt gaagcgttag agagtttcga gagaggccga acgtgcgcgc tagacgtaaa
     4321 cgactttcct caatttgcgt cactccgtcc tgcagtcgcg gctgcgtagc acgccgggac
     4381 cactcgacca cgcggccgca ccgcattgag cataattgta tacatagagt ccaaagaatt
     4441 caatggctcg ggcgagcgct agggctgggg acgcagccag ccactagcca ccagcactag
     4501 ccactccttc ccagccccag cccccagccc ccgccctatg cgccccgcca ttcgcgttga
     4561 cggcctgccg aagcactacc gcgtgagtgc gcgccaacgg ggctatacga cgctccgcga
     4621 gtcgatcgct gaggcgttct ggtcgccgct gaagcggctc caagggctag ggctagtggg
     4681 taaggctagg gaagcggcag cagacaagag tccctagtcc tcgacaataa agcggagact
     4741 ctgtttgttt actgaactta ttaagcgcct tattggacgc ttcggctacg acatccggta
     4801 ttcaccgaca accgtggggg agtgcgccgg cagatactgg atcctcgata ttcagcggct
     4861 cttgaccgct caatcgccac taatcttgga tattggtgcc aatgagggcc aaacggctcg
     4921 cgaattcaag gcgcgctggc cacaggcttc ggtgcactca ttcgagccag ctccggagac
     4981 atttgaccga ttagcggcca cggcgaagag tctgaaggga gtaaccgcca acaacgttgc
     5041 cctcggagca aagccaggaa cggcgccctt gatttgtcat cgtcattccg ttctgaactc
     5101 gatcttgccg ccgggacgag attacgacac ctgggaaaca ttgggaacat gcgaggttga
     5161 agtatcaacc gtggacacgt attgcgccga acgcgggatt gagcgaattg atgtgcttaa
     5221 gacggacacc caggggtttg acctcgaagt tctgcgaggt tcacgggaaa tgttcgtcgg
     5281 gaatcgcgtt catcttgtgt ttgccgagat tacattcacc gagatgtacg acgggcaggc
     5341 cagatacgat caggttttcg gcattttagc cgatcacggg ttctcaatcg tgtccctata
     5401 cgatctcaga taccgcgatc atcgcttgga atggtgcaat gccctgttcg taaattccaa
     5461 ctggcagagt acacacatcg gc
//
LOCUS       DASRJB010000566         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4109455, whole genome shotgun sequence.
ACCESSION   DASRJB010000566 DASRJB010000000
VERSION     DASRJB010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4109455"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFB80_14795"
     CDS             complement(<1..369)
                     /locus_tag="VFB80_14795"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN35094.1"
                     /translation="MSAAAAVAAPVQNERAARVADPRIVLRDVPWETYQILRRAEANN
                     HLRMIYDRGNLEIMSPLKRHGKIATLLDHMFHAWTLHCGTEIESGRDMTCDREDMKKG
                     LEPDLCYWTIHQPLVHGKDEV"
     gene            complement(460..>3937)
                     /gene="smc"
                     /locus_tag="VFB80_14800"
     CDS             complement(460..>3937)
                     /gene="smc"
                     /locus_tag="VFB80_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZN35095.1"
                     /translation="LIFDNSEKKLPVDAPEVHITRRVYRSGEGEYLINRQPARLKDIR
                     DMFRGTGVGADAYSLIEQGKVDKLLQASARDRRAIFEEAAGISRFKAKKVEAQRRLER
                     VEQNLLRLSDIVEEVENRLKTVRAQAGKARRYREYSERLQQLRTQIALVDWRRLTEQL
                     AQIETELVALAEQIQTADAETQRLEAQQLELETAAAAAAEELHNCENQLGFLRQHSAQ
                     HQATIDHERLRGVELEEAARLHGKNLLAMSDKAGDVRSRLKATQAEVAAAQASHAVAA
                     TALCDREGHLVELSRRQQQARQQAESRRQQYMAQMQAAATLASQVEGGQTQLAAVQQA
                     VIQLQRQLEELAGSEADLAAEQATSHERERQLAEQLAAHQQQVEAVTRDLAENRNLHA
                     SRAGDLSQLHGRQRGIVERAEVLAEFERTLEGIGAGVKELLARAGVKSQEPGASGQEP
                     VARASGLPWSSVRGVVADFVQVGVEHAAAIDVALGDAAQAVVIEGGEVEASALIAVAS
                     DLAGRVMLIPSPSSPPSHQPPATSHFPAPSIELTADMASGVHVIARADKLALCPDEMR
                     PLVAQLLGRTFIVRTLDDAFRLRAKAPPGTRVVTAAGELLDEQGAVAFGPRQSATSLV
                     SRRSQLRAARLDLAVLDQQIEDAQRETAHLKREIERHEAELARLHEQGKSLDQQVASE
                     GATQRTLALRAAQLEQQTAAAQRSLNESKIRRQSLEAGLAQASRNLVVAQSAAGQFQA
                     AIAGQEQELLELDAERQQAAGEATAAKIELARCDQRLESLRLRLTQFEDDSRERERAI
                     AQVRQQWKQAQQRQEASQLAALAAGAELAELALKQQSLGRQMCDCLARRDAAASGRAA
                     LVEQLSGLRRRTAKLHSEEHQQQLAAEQVRHERKTLADRMRDDYSIDLAAAAAESATH
                     QAAEERAAVEQEIESLRRKIQQIGAVNLDALDELDELEQRFATLSAQHKDLTDAKESL
                     ARIIHKINADSRRLFLETLEAIRVNFQALYRKAFGGGKADIVLEEGVDVLDCGVEIIA
                     TPPGKPSFNNSLLSGGEKALTAVALLLAIFQFRPSPFCVLDEVDAPFDEANIGRFIDV
                     LKDFLGWTRFVIVTHSKKTMTAATTLYGVTMQESGVSKRVSVRFDDVTDDGHIRQAAL
                     DRAGDEAAENAA"
BASE COUNT          523 a         1361 c         1312 g          741 t
ORIGIN      
        1 gacttcatcc ttgccatgca ccagcggctg atggatcgtc cagtaacaga gatcgggctc
       61 caatcccttt ttcatgtcct cgcggtcgca ggtcatgtcg cgacccgatt cgatttcagt
      121 gccgcagtga agtgtccagg catgaaacat gtgatcgagc agggtggcga tctttccgtg
      181 ccgcttgagt ggagacatga tttcgaggtt tccgcgatcg taaatcatcc gcaagtggtt
      241 gttcgcttcg gcccggcgga ggatctggta cgtctcccag ggcacgtcgc gaagaacgat
      301 ccgaggatct gcaaccctcg cggccctctc gttctggacc ggagctgcaa ccgcggcggc
      361 agcactcata gtctcgctcc cagtgtcggc aggcgtccaa cccgacgcct actattgtac
      421 acctgcccgg ccgaaaaggg aatccgcgtc gccgcgcacc tacgccgcgt tctcggccgc
      481 ctcgtcgccg gcgcggtcga gggccgcctg gcggatgtgc ccgtcgtcgg tgacgtcgtc
      541 gaagcggacg ctgacccgct tggagacgcc ggactcctgc atcgtcacgc cgtacagcgt
      601 ggtggcggcg gtcatcgtct tcttgctgtg cgtgacgatc acgaaccgcg tccagccgag
      661 gaaatccttg agcacgtcga tgaaccggcc gatgttggcc tcgtcgaacg gcgcatcgac
      721 ctcatcgagc acgcagaacg ggctggggcg gaattgaaag atggccagga gcagggccac
      781 cgctgtgagg gccttttcgc cgccggagag cagcgagttg ttgaacgacg gcttgcccgg
      841 cggcgtggcg atgatctcga cgccgcaatc cagcacatcg actccctctt ccagcacgat
      901 gtcggccttg ccgccgccga acgccttgcg atagagggcc tgaaagttaa cgcggattgc
      961 ctccagcgtt tccaggaaca gccgccggct gtcggcgttg attttgtgga tgatccgggc
     1021 cagcgactcc ttggcgtcgg tcaggtcctt gtgctgggcc gagagcgtcg cgaatcgctg
     1081 ttccaattcg tccagctcgt ccagcgcgtc gaggttgacc gcgccgatct gttgaatctt
     1141 gcggcggagc gattcgatct cctgctcgac cgcggcccgc tcctcggcgg cctggtgggt
     1201 cgccgactcg gcggccgcgg cggccaggtc gatgctgtaa tcgtcgcgca tccggtcggc
     1261 aagcgtcttc cgctcgtggc ggacctgctc ggcggccagc tgctgctggt gctcctcgct
     1321 gtgcagtttg gccgtgcggc ggcggagccc gctgagctgc tcgacgagcg ccgcgcggcc
     1381 ggacgcggcg gcgtcgcgcc gggccagaca gtcgcacatc tggcggccga gcgactgttg
     1441 cttgagtgcc agctccgcca gctcggcccc agcggccagc gcggccagct gcgaggcctc
     1501 ctgccgctgc tgagcctgtt tccattgctg ccggacctgt gcaattgccc gctcgcgctc
     1561 gcggctgtcg tcttcgaatt gcgtcaggcg gagcctcagg ctctccaatc gctggtcgca
     1621 gcgagccagt tcgatcttgg cggcggtagc ctcgccggcg gcttgctggc gctcggcgtc
     1681 gagctccagc agctcctgct cttgcccggc gatcgccgct tgaaactgcc cggcggcgga
     1741 ctgcgcgacg accaggttcc ggctggcctg cgccagaccg gcctcgagcg actgccgccg
     1801 gatcttcgat tcgttgagcg atcgctgcgc cgcagccgtt tgctgctcga gctgcgcagc
     1861 gcgaagcgcg agcgtccgct gcgtcgcccc ctcgctagcc acctgctggt cgagcgattt
     1921 gccctgctcg tgcagccgag cgagctccgc ctcgtgccgc tcgatctcgc gcttgaggtg
     1981 ggccgtttcg cgctgggcgt cttcgatctg ctgatcgagt acggccaggt cgaggcgggc
     2041 cgcccgcagc tggctccgcc gcgagacgag gctcgtcgcc gactggcgcg ggccgaaggc
     2101 gaccgcgccc tgctcgtcga gcagttcgcc ggcggcggtg acgacgcgcg tgcccggcgg
     2161 cgctttggcc cgcaggcgga aggcgtcgtc cagcgtgcgg acaatgaacg tgcggccgag
     2221 cagctgagcc acgagcggcc gcatttcatc cgggcacagg gccagcttat cggcccgggc
     2281 gatgacgtga acgccgctgg ccatgtcggc ggtgagctcg atggaggggg cggggaagtg
     2341 gctagtggct ggcggctggt ggctaggggg cgacgacggc gacgggatca acatcactcg
     2401 tcctgccaaa tcgctcgcca cagcaatcag ggccgacgct tcgacttctc caccttcgat
     2461 gacgaccgcc tgagccgcgt cgcccagggc cacgtcgatc gctgcggcgt gttcgacacc
     2521 gacctgcacg aagtcggcca caactccgcg gacactcgac cagggcaggc cagaggcccg
     2581 cgccactggc tcctggccac tggctcctgg ctcctgactc ttgactcccg ccctggccag
     2641 cagttccttc acgcccgcgc cgatgccttc cagcgtacgc tcgaattcgg cgagcacttc
     2701 ggcccgctcg acaatgccgc gctggcggcc gtgcagctgt gacaggtcgc ccgcgcgcga
     2761 ggcatgcagg ttgcggttct cggccaggtc acgcgtgacg gcttccacct gctgctgatg
     2821 tgccgcgagc tgctccgcca attgtcgctc gcgctcgtgg ctagtggcct gctcggcggc
     2881 gaggtccgct tcgctcccgg ccagctcttc cagctgccgc tggagttgga tcacggcctg
     2941 ctgcaccgcg gccagctgcg tctggccccc ttccacctga ctggccagcg ttgcggccgc
     3001 ctgcatctga gccatgtact gctgccgccg gctctcggcc tgctgacggg cctgctgttg
     3061 ccgccggctg agctcgacca gatgtccctc gcgatcgcaa agagcggtcg ccgccacggc
     3121 gtgcgacgcc tgggccgcgg ccacttcggc ctgcgtcgcc ttgagccgcg agcgcacgtc
     3181 gcccgccttg tcgctcatcg ccagcaggtt tttgccgtgc agcctggccg cctcttccag
     3241 ctcgacgccg cgcaagcgtt cgtgatcgat cgtcgcctgg tgctgggcgg agtgctgccg
     3301 cagaaagccg agctggttct cgcagttgtg caattcctcg gccgcggcgg cggctgccgt
     3361 ttccagctcc agctgctgcg cctccagccg ctgcgtttcc gcgtcggccg tttgaatctg
     3421 ctcggcgaga gcgaccagct cagtttcaat ctgggccagt tgctcggtca gccgccgcca
     3481 atcgaccagg gcgatctggg tgcgcagctg ctgcagccgc tcgctgtatt cgcggtagcg
     3541 gcgagccttg ccggcctggg cccgcaccgt cttcaggcgg ttctcgacct cctcgacgat
     3601 gtccgacagc cgcagcaggt tctgctcgac tcgctccagc cgccgctggg cctcgacctt
     3661 cttggctttg aagcggctga tgccggcggc ctcctcgaag atcgcccggc ggtccctggc
     3721 cgaggcctgc agcagcttat cgaccttgcc ctgctcgatc aggctgtagg cgtcggcccc
     3781 gacgcctgtg ccgcggaaca tgtcgcggat gtctttcagc cgggcgggct gccggttgat
     3841 caggtactcg ccttcgccgc tgcgatagac gcggcgcgtg atgtggacct cgggggcatc
     3901 gacggggagc ttcttctcgc tgttgtcgaa gatcagc
//
LOCUS       DASRJB010000567         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2982960, whole genome shotgun sequence.
ACCESSION   DASRJB010000567 DASRJB010000000
VERSION     DASRJB010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982960"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2914
                     /locus_tag="VFB80_14805"
     CDS             <1..2914
                     /locus_tag="VFB80_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35096.1"
                     /translation="VIARFEAERQALALMDHANIARVLDAGATPASRPYFVMDLVRGV
                     PITRYCDEHRLTPRQRLELFIPVCQAVQHAHQKGIIHRDLKPSNVLVAQYDGRPVPKV
                     IDFGVAKAAGQPLTEKTLVTGFGAIVGSLEYMSPEQAEINQLDIDTRSDIYSLGVLLY
                     ELLTGSPPFSRAAQTPAGMLEMLRLIREQEPTRPSTKLSTAEGLPTLAANRGTEPAKL
                     TRLLRGELDWIVMKALEKDRSRRYETATSLAHDLERYLADEPVLACPPSASYRLWKFA
                     RRHKAALGTAAVVAAAILLAVGSLGWAARDRAAGRLKSAGEVNAFLARAELHYADNEL
                     PEALAEVEKAKGLLEPGDVRGPLKHRVEQWRKDLETAMRLEEIRLDTLSGNQRKRRYG
                     DVVQAIREYGIDIEALPVEQAAARIKASQIKLDLALTLGSTAEGLQFGERPASTPWRR
                     LLAISQAVDPDSLQMRLYEAVAAKDVQTLRDLASDADPARLRTRTLAQLGNSLSTAGE
                     LQSAVDFLRRAQRQHPSDFSINLQLAYALIRTGQPSREVTAFRRAALAARPKSALAHQ
                     QLAVALFAEQRPDEAVLICQQALALDPSYAPAYYTLGDALAMQEKLNDAIAAYRAAIV
                     RNPTDAEAHFALGRTLERHGDPAEALDKYRAAFNLDPTVVGLSPALGRLLRNYGRLEE
                     AIDVFRNAVDQQRDTNKTTAQKDILRRGFADVLVSRAWQLATATDLQQRDPARALELA
                     LEASTLVPGYTSYWRPLGVAQYRLGDYHAAAQTLGMVRQQAPDFNDSVYLFFLSMAYA
                     RLNQAEKARELFNVAAQWRLSDYTADAEVRKLCAEAAEVLGLAVPPDLQEPPVLAKGP
                     TLLAPASSGTLPNGTLGAKQPMVWEFDWSDVPGATRYHLSVKAARADAPSVSQSKLAA
                     SSYRYESKSYVPEPNRTGWRWKVRALVDDVWTDWSQEGTFTVEPIDLGAAATPKGR"
BASE COUNT          582 a         1036 c         1044 g          526 t
ORIGIN      
        1 agtcatcgcc cgctttgagg ccgagcggca ggcgctggcc ctgatggacc acgccaacat
       61 cgcgcgtgtg ctggacgctg gtgcgacgcc agccagccgg ccgtacttcg tgatggacct
      121 ggttcgcggc gttccgatca ctcgctactg cgacgagcac cgcctcacgc cccgccagcg
      181 gctggagctg ttcatccctg tttgccaggc ggtccagcac gcccatcaaa agggcatcat
      241 ccaccgcgac ctcaagccgt cgaacgtgct cgtcgcccag tacgacggcc ggcccgtgcc
      301 caaggtcatc gactttggtg tggccaaggc ggctggtcag cccctcaccg aaaagacgct
      361 ggtcacgggc ttcggcgcga tcgtcggctc gctggaatat atgtcgcccg agcaggccga
      421 gatcaaccag ctcgacatcg acacgcggag cgacatctat tcgctgggcg tgctgctgta
      481 tgagctattg accggcagcc cgccgttcag ccgcgcggcg cagacgccgg ccggcatgct
      541 ggagatgctg cggctgatcc gcgagcagga gccgaccagg ccgagcacca agctgagcac
      601 ggcggagggc ttaccgacgc tggctgccaa tcgcggcacg gaacccgcga agctgaccag
      661 gctgcttcgc ggcgagctcg actggatcgt gatgaaggcc ctggagaagg accgcagccg
      721 ccgctacgag acggcaacca gcctcgccca cgacctggag cgctacctgg cagatgagcc
      781 ggtgctggcc tgcccgccgt cggccagcta tcggctgtgg aagttcgccc gccggcacaa
      841 ggctgccttg ggcaccgccg cggtggtcgc cgcggccatc ctgcttgccg tcggcagtct
      901 tggctgggcg gcgcgcgacc gcgccgcagg ccgactcaag tcggctggtg aggtgaacgc
      961 gtttctcgcg agggccgagc tccattacgc cgacaacgag ctgcccgaag cgctggctga
     1021 ggtcgagaaa gccaaagggt tgctcgagcc gggcgacgtc cgcgggccct taaagcaccg
     1081 cgtggagcag tggcggaaag acctggaaac ggccatgaga ctggaggaga tccggctcga
     1141 taccctttct gggaaccagc gcaaacggcg atacggggat gtcgtccagg cgattcgcga
     1201 atacggcatt gacattgagg cactgcccgt tgaacaagcc gcggcgcgga tcaaggccag
     1261 ccagatcaag ctcgacttgg cgcttacact gggcagcacc gcggaggggc tgcaatttgg
     1321 cgagcggccg gcttcaacgc cctggcggcg gctgcttgcc atttcgcagg ccgtcgatcc
     1381 ggattcattg caaatgcggc tgtatgaagc ggtggcggcc aaggacgttc aaacgctccg
     1441 cgatctggca agtgatgctg acccggcgcg gctccgcacg cgaaccctcg cccagctcgg
     1501 gaacagcctg agcacggccg gcgagctgca gtcagccgtt gactttcttc gtcgggccca
     1561 gcggcagcac cccagcgact tttcgatcaa cttgcaactg gcctacgcgc tgattcgaac
     1621 aggacagcca tcgagggagg ttaccgcgtt tcgccgggcg gccctggccg cccggccgaa
     1681 atcggcgctt gcgcaccagc agctggccgt cgcgctcttc gccgagcagc gaccggacga
     1741 ggccgttctg atttgtcagc aggccttggc gctcgatccg agttacgccc ctgcttacta
     1801 cacgctgggc gacgccctgg cgatgcagga aaagctgaac gacgccatcg ccgcgtaccg
     1861 cgcggccatc gtgcggaatc cgacagacgc tgaggcccat ttcgccctgg gccgcacgct
     1921 ggaacgccat ggcgacccag cagaggcact cgacaagtac cgggcagctt ttaacctcga
     1981 tccgacggtt gtcggccttt caccggcgct ggggcgattg ctccgtaatt acggccggct
     2041 ggaagaggct attgatgtgt ttcgcaacgc ggtcgatcag caaagggaca ccaacaaaac
     2101 cacggctcaa aaggacattc tccgaagggg cttcgccgac gtcctggttt cgcgggcctg
     2161 gcaactggcg accgccaccg acctccagca gcgggatccg gcccgggcgc tcgaactggc
     2221 attggaagcc agtacgctgg tgcccgggta caccagctat tggcggcctc tgggagtcgc
     2281 gcagtatcgc ctaggggact atcacgcggc cgcgcagacg ctgggaatgg tgcggcagca
     2341 agcgcctgac ttcaacgaca gcgtctacct tttcttcctg agcatggcct atgcccggct
     2401 gaatcaggcc gagaaggctc gcgagttatt taacgtggcc gcccaatgga gattgagcga
     2461 ctacaccgcg gacgccgagg tgcggaagct gtgcgccgaa gctgcggaag tgctgggtct
     2521 ggcagttccg cccgacttgc aggagccgcc ggtgctggcc aaagggccta ccctgctagc
     2581 gcccgcctcc agtggcactt tgcccaacgg caccttgggc gcgaagcagc ccatggtctg
     2641 ggagttcgat tggtccgacg tgccgggtgc gacccggtat cacctgtccg tcaaagcggc
     2701 cagagccgac gcacccagcg tcagccaatc gaagctcgca gcgtcatcct accgctacga
     2761 aagcaagtcc tatgtccccg agccgaatcg caccggctgg cgctggaagg tgcgggccct
     2821 ggtggacgat gtttggaccg attggagcca ggaggggacc tttacggtgg agccgatcga
     2881 tctgggtgcc gccgcgacgc cgaagggccg ctgaggccgg tccgtccgac tgaggcggcc
     2941 caagctgaat tctgggaggg gttacatcga gtcagaacgg ctgaccgcgg attccggtcg
     3001 gcccggattt tcgaaatgat cttttgcgct tgtcaggtcg caatctggca tatcgatatc
     3061 gagggaagca cccttgaggt cccgccgtga acgagcgtga acttttcatc gccactctgc
     3121 agctcgacga aagccggcgc gcagactact tgaacaaggc ctgcaccggc gatgcctcca
     3181 tgcgccag
//
LOCUS       DASRJB010000568         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_160416, whole genome shotgun sequence.
ACCESSION   DASRJB010000568 DASRJB010000000
VERSION     DASRJB010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160416"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFB80_14810"
     CDS             complement(<1..174)
                     /locus_tag="VFB80_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZN35097.1"
                     /translation="MEKLVSLCKRRGFLFQSSEIYGGLNGFWDYGPLGVELKRNIKEA
                     WWRDMVIGHDDLAP"
     gene            complement(366..1142)
                     /locus_tag="VFB80_14815"
     CDS             complement(366..1142)
                     /locus_tag="VFB80_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HZN35098.1"
                     /translation="MNARSFSELTMPAERQLDVYLLPALVEPAELAGKTAVVIDVLRA
                     TTTVIHALACGATQVLPCLEVEETLETAAACLLRGESAIRGGERGGRQIPGFELGNSP
                     AEYSRQVVGGKTVVFTTTNGTRAMLRCRQARRVLIGAFVNFSAVCRELGGESRIALVC
                     AGTDGHVTREDTLLAGAIASDLAREGSWRLNDQAEIAADSWQTAVRLMTDRPLGILLR
                     GSRGGRNLIDIGQENDIDLAAQIDKFDIVPELDLLAWRIR"
     gene            1279..2100
                     /locus_tag="VFB80_14820"
     CDS             1279..2100
                     /locus_tag="VFB80_14820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN35099.1"
                     /translation="MSSAPSTSTSPQHSSAERRFAGKVALITGASDRGIGGEIAVRLA
                     REGAAVAMISRSVPERLLKKVGRFQHGVVHTAGDVTKRDDIARALDACLGEFGKLDVV
                     VNNAGVELARSLEKHDEAEWKQLLDINLNGAIAVSQAALPCLSSPGGVIVNIASALGL
                     GGCPGFSIYSASKAGLIGFTQSLAWELAPKGIRVVAVAPGLVHTPMIHKHVNQLTPET
                     WAKIEACHPLGMGRPDDVAAAVAFLASADARWITGITLPLGWAYHYPLPTGQMMG"
     gene            2186..3610
                     /locus_tag="VFB80_14825"
     CDS             2186..3610
                     /locus_tag="VFB80_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZN35100.1"
                     /translation="MPRSAVQLRADALAIWHAAVAAVRSDRLVQENVWVEGDWLHVGD
                     ETLDLPSIGRIAVVGAGKAGAGMAAGLAAALGPQLLAAKQLAGWVNVPADCIPADTNP
                     TRQRGSPIHLHAARPAGINEPTTDGVYGAEKILEIVRGLGPQDLCFCLISGGGSALLP
                     APVEGVSLAEKQALTRLLSAGGANIQELNTVRKQLSRIKGGGLARACRAGRLISLIIS
                     DVLGDPLDVIASGPTVTDRSTPAEALAILERYLRGNESLAPAALAYLRAQAVVRGANH
                     SHTPSNVTNRVIGNNAVAVDAAGVEAERRGYSHAMDAATKLEGPAEEVGRHLARMALR
                     MQSEPGPDCLITGGEPTVQLAPAEIRGKGGRNQQLVLAAVVELLPSLALAPSPSPLCL
                     LSGGTDGEDGPTDAAGAFLDAETFVRVGDRNLDPADYLRRNDAYRFFQETGGLIHTGP
                     THTNVCDVRVVVVDRRQDSPGSRI"
     gene            3818..4462
                     /locus_tag="VFB80_14830"
     CDS             3818..4462
                     /locus_tag="VFB80_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35101.1"
                     /translation="MASTQPDQVGVIRSADSGPASHAADLALVQRLAGGDAAAWREFV
                     ERYQRLILTRVLVAAREANQPLAVADAEDQTAEVFAALVAGGFAMLRRFEGRSTLSTW
                     LCVVTRRMVLRRLSARRRDPARPAAGPPLDALAGPPHDSPLTKLIGGEDLDRLSAGLD
                     QLGERQQQIARLFYWGGCTYREISEQLDMPVNSIGPTLARIQQRLRAALGEEDP"
     gene            4459..>5199
                     /locus_tag="VFB80_14835"
     CDS             4459..>5199
                     /locus_tag="VFB80_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35102.1"
                     /translation="MNHPAPDLLTLLDWSQRLARGEHCAEWKAWRESSPDAAWQWPRV
                     ALAVDLLDAGELPEMAAEDVAAWLDGGLSEDAAAAAEQCCWHSPAQLAEVASATSFRD
                     PATLLAAPTEDLTRRLLELGPQPAMWRLDGAQKSLPVASPLPPPVAEQVPLAVVQPKP
                     ARPRRSADLPLWVLISAAALLLVALSGVIGWFATSSAPRGPGPGPVVEGPGERPAPPP
                     PWPLPVPRPPEAPREPAPSSSPPQVESPQ"
BASE COUNT          836 a         1837 c         1731 g          795 t
ORIGIN      
        1 cggcgcgagg tcgtcgtggc cgatgaccat gtcccgccac caggcttcct tgatgttccg
       61 cttcagctcg acgcccaatg ggccatagtc ccagaagccg ttgagcccgc cgtaaatctc
      121 gctcgactga aacaaaaatc cgcgccgctt gcacagcgag acgagctttt ccatttccat
      181 gggagggttc agagttcagg gttcagttct tcagccctcg ttcccgagcc tccgcctggg
      241 aacgcacttc cgcgaggctc ctgcctcgcc gacaacaaca gacgccagtt taccggtgaa
      301 ggttcaaagc cgcgaccact ggtcgcggtc tgcgggagaa ccgcgaccag tggtcgcggc
      361 cttgactagc gaatccgcca cgccagcagg tcgagttccg gcacgatatc gaacttgtcg
      421 atctgggcgg ccaagtcgat gtcgttctcc tggccgatgt cgatcaggtt ccgcccgccg
      481 cggctgccgc ggagcaggat gcccagcggc cggtcggtca tcagccgcac ggccgtttgc
      541 cagctgtcgg ccgcgatctc ggcctggtcg ttcaagcgcc acgagccctc gcgggccagg
      601 tcgctggcga tcgctccagc cagcagcgta tcctcgcgcg tcacgtgccc gtcggtgccc
      661 gcacagacca gcgcgatccg agactcgccg cccaattccc ggcagaccgc ggagaaattg
      721 acgaaggccc cgatcagcac ccgccgtgcc tgccggcagc gcagcatcgc ccgcgtgccg
      781 ttcgttgtcg tgaacacgac cgtcttgccg ccgacaacct gtcggctgta ttccgccggc
      841 gagttgccca gctcaaaccc gggaatttgc cggccgccgc gttcaccgcc gcgaatcgcg
      901 gactcgccgc gcagcaaaca cgcagccgcc gtctcgagtg tctcttcaac ctcaaggcag
      961 ggcagcactt gcgttgcgcc gcaagccagg gcgtgaatga ctgtcgtcgt ggcccgcagc
     1021 acgtcgataa ccacggcggt ttttccggcc aattccgcag gctcgaccag cgccgggagc
     1081 aaatagacgt caagctgccg ctcagccggc atagtcaact ccgaaaacga acgggcgttc
     1141 attgacgccg cccttaaccg ctcagaaaat cagtctagcg gccgataaac tgccatcgta
     1201 gggtgcatga acggagcgaa atgcaccttg tcacccactc aggtgcattg cgctgcgcta
     1261 atgcacgcta ccaggctcat gtcctccgcc cctagcacca gcactagccc ccagcactct
     1321 tcggcagagc ggcgtttcgc cggcaaggtc gccctgatca ccggggcgag cgaccgcggt
     1381 attggcggcg agatcgcggt tcgcctggcc cgggaagggg cggccgtcgc gatgatttcg
     1441 cgcagcgtgc ccgagcggct gctcaagaaa gtcggccgct tccagcacgg cgtggtgcac
     1501 acggccggcg acgtcaccaa gcgcgacgac atcgcgcgag ccctcgacgc gtgcttgggc
     1561 gagttcggca agctcgacgt ggtcgtgaac aacgccggcg tcgaactggc ccgctcgctc
     1621 gagaaacacg acgaggccga gtggaagcag ctgctcgaca tcaacctcaa tggcgcgatc
     1681 gccgtttcgc aggcggcgct gccgtgcctc tcttcgcctg gaggcgtaat cgtgaacatc
     1741 gcctcggcct tgggcctcgg cggctgtccg ggcttctcga tttacagcgc gagcaaggcc
     1801 gggctgatcg gatttacgca gtcgctcgcc tgggagctcg cgcccaaggg cattcgcgtc
     1861 gtagcggtcg cgccaggcct ggtgcacacg ccgatgattc acaagcacgt caatcagctc
     1921 acgcccgaaa cgtgggccaa gatcgaggcc tgccatccgc tcggcatggg ccgccccgac
     1981 gacgtggccg ccgccgtcgc cttcctcgcc tcggccgacg cccgctggat caccggcatc
     2041 acgctcccgc ttggctgggc ctaccactac ccgctgccga ccgggcagat gatggggtaa
     2101 ggtgcgctgt agtgtacgcg ctccgcggcg tgccgagcct ccggtaacgc cggagaacgc
     2161 cacagagggc gttccctaca gaaacatgcc ccgctctgcc gtccaactcc gcgccgacgc
     2221 cctcgccatc tggcacgccg ccgtcgccgc ggtgcggagc gatcggctgg tgcaggaaaa
     2281 cgtgtgggtc gagggggact ggctgcatgt tggggatgaa acgctggatt tgccgtcgat
     2341 cggccgaatc gcggtcgtcg gcgcgggcaa ggcaggagcg ggaatggccg cgggcctcgc
     2401 ggcggcgctg gggccgcaac tgctggccgc caagcagctc gccggctggg tgaatgtccc
     2461 ggcggactgt attcccgccg acactaaccc gacgcgccag cgagggagtc cgatccactt
     2521 gcacgcggcc cgccccgctg gcatcaacga gccgacaacc gacggcgtct acggggcgga
     2581 gaagatcctc gagatcgtcc gcggcctcgg cccgcaagac ttgtgcttct gcctgatttc
     2641 gggcggcggc tcggcgctgt tgccggcgcc ggtggaaggc gtctcgctgg ccgaaaaaca
     2701 agcgctgacg cggctgctga gcgcaggcgg ggcgaacatc caggaattaa acaccgtccg
     2761 caaacagctt agccggatca aggggggcgg gctggcccgg gcctgccgcg cgggacgcct
     2821 catttcgttg atcatctcgg acgtgctggg cgatccgctc gacgtgattg cgtcggggcc
     2881 gaccgtgacg gatcgatcca cgccagccga ggcgctggcg attctggagc gatatttgcg
     2941 cggcaacgaa tcgctcgcgc cggcggcgct ggcctatttg cgtgcacagg ctgtggttcg
     3001 cggcgcgaac cacagccaca cacccagcaa cgtcaccaac cgtgtcatcg gcaacaacgc
     3061 cgtcgccgtc gatgccgccg gagtggaagc cgagcgccgc ggctattcac acgcgatgga
     3121 cgcagccaca aagctcgagg gcccggccga agaggtcggc cggcacctgg cccgcatggc
     3181 cctgcggatg cagtccgagc ccggccccga ctgcctcatc accggcggcg aaccgaccgt
     3241 gcagctcgcc cccgccgaaa tccggggcaa gggagggaga aatcagcagc tcgttctggc
     3301 ggcggtggtt gagctattgc cttccctagc cctagccccc agccctagcc ctctctgcct
     3361 cctctccggc ggcaccgacg gcgaagacgg cccgaccgac gccgctggcg cgtttctgga
     3421 cgccgagacg tttgtgagag tcggcgaccg caacctcgat ccggccgact atctccgccg
     3481 caacgacgcg taccgcttct tccaagagac cggcggcctg atccacaccg gcccgaccca
     3541 caccaacgtc tgcgacgtgc gggtggtcgt cgtcgatcgc cgtcaggaca gcccgggatc
     3601 gagaatctga aaaaaagtcg cctcggactg tacgaaggct gccgcgattg acactaatgg
     3661 agtatggcgg tttgcgccgc cacgggacta gtgagaagag gcgcgtgctt gcggcggctc
     3721 atccagaggc gagccgccgt accgtccgcc gagggcgggc ggccgcgcct gacggctcga
     3781 ttccgagtcc gcacgtgcgt ccgccgaggc ttcggccgtg gccagcaccc agccagacca
     3841 ggtaggagta atccgctccg cggacagcgg ccctgcctcg cacgcggccg atctggcgct
     3901 cgtgcagcgg ctggccggcg gcgacgccgc ggcctggcgc gagttcgtcg agcgctacca
     3961 gcggctgatc ctgacccgcg tgctggtggc ggcccgcgag gcgaaccagc ccctggcggt
     4021 ggccgacgcc gaggaccaaa cggccgaggt cttcgcggcg ctggtcgcgg gcgggttcgc
     4081 aatgctgcgg cggttcgagg ggcggagcac cctctcgacg tggctctgcg tggtgacgcg
     4141 gcggatggtc ctgcgccgac tcagtgcccg ccggcgcgat cccgcgcggc cggccgccgg
     4201 gccgccgctg gacgccctcg ccggcccgcc gcacgacagc ccgctaacaa aactgatcgg
     4261 cggcgaggac ctcgaccggc tctccgccgg gctggatcaa ctcggcgagc gacagcagca
     4321 aatcgcccgc ttgttttact ggggcggctg cacctaccgc gaaatcagcg agcagctcga
     4381 catgcccgtg aactccatcg gcccgacgct ggcccgcatt cagcagcggc tccgcgcggc
     4441 ccttggcgaa gaggacccat gaaccatccg gcgcccgatc tcctgacgct tctcgactgg
     4501 tcgcagcggc tggccagagg agagcactgc gccgagtgga aagcctggcg cgagtcgtcg
     4561 ccggacgccg cctggcagtg gccgcgcgtc gcgctggccg tggacctgct cgatgcgggc
     4621 gagctgccgg aaatggcggc cgaagacgtc gcggcctggc tcgacggcgg cttgagcgaa
     4681 gatgcggcgg cggccgctga gcaatgctgc tggcattcgc cggcgcagct cgccgaagtc
     4741 gccagcgcca cgagcttccg cgatccggcg acgctcctgg ctgcgccaac cgaagatcta
     4801 acccgccggc tgctggagct gggcccgcag cccgcgatgt ggcggctcga cggcgcgcag
     4861 aagtcgctgc cggtcgcatc gcctcttccg ccgccggtcg cagagcaggt gccgctcgcc
     4921 gtggttcagc ccaagccggc caggccgcgg cggagcgccg acttgccatt gtgggtgctg
     4981 atcagcgctg ccgcgctgtt gctggtcgcg ctgagcggcg tgatcggctg gttcgcaaca
     5041 agcagcgcgc cgcgtggtcc tggtccgggg cctgtcgtcg agggcccggg cgagaggccc
     5101 gctccgccgc ccccgtggcc gttgcccgtg ccgcgcccgc ccgaagcacc gcgcgagcca
     5161 gcgccttcgt cctctccgcc gcaagtcgaa tcgccgcag
//
LOCUS       DASRJB010000569        12784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2700999, whole genome shotgun sequence.
ACCESSION   DASRJB010000569 DASRJB010000000
VERSION     DASRJB010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700999"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFB80_14840"
     CDS             <1..1006
                     /locus_tag="VFB80_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35103.1"
                     /translation="TENQRLFDIEVISSGHRFPMGMARNRDGELFVTDNQGNYNPFNE
                     LNHVRKGAHFGFINALEKEQGFRPPPLAEPAINIPHPWTRSVNGICFLETPVGWVKDA
                     ERPQTHAGTASEAGAPRGSSAAAASTHPTMFGPLEGQLVGCEYDTRRLIRMSLQKVGD
                     TYQGAAYPLSVPPAEVEKGLLGPIVCAIKPTTAELYVGEIHDSGWGAGNNIGQIVKIK
                     IEPEKLPCGIAEVRATSGGFAIDFMQPVDRAKAADLASYAIESYRRESTPAYGGPDID
                     RRIEKIGSAEVAADGKRVMLTLPNLRAGFVYELKLKNLAPAGSVFFPDEAHYTLHKIP
                     "
     gene            complement(1032..2018)
                     /gene="fmt"
                     /locus_tag="VFB80_14845"
     CDS             complement(1032..2018)
                     /gene="fmt"
                     /locus_tag="VFB80_14845"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZN35104.1"
                     /translation="MRIVVLGTGPFAVPLFQSLVDSRHEILALITRPTPPAKGREKAP
                     PNPMRDLAEQRGLTVHAPESVNSEEGLILIRSLQPDLLVVCDYGQILSAEALGIARLG
                     GINVHASLLPKYRGAAPIQWALLHGESETGVSVIHMTPRLDGGPILAVRATLISSDET
                     QLALEDRLAHLGIEAVHEAIDLLGKWDRASPIGTPQDPQLATKAPRLKKEHGLIDWQR
                     TAEQIRNQVRALKPWPGTFTFWSRPGHEPLRIVIDRVSILGLGIRAAAMVDSSAPVHQ
                     PGEVVVSDRQQLIVATGDGGLSLINVQPAGKRVMPVADFLHGYHIREGERLG"
     gene            complement(2085..2666)
                     /gene="def"
                     /locus_tag="VFB80_14850"
     CDS             complement(2085..2666)
                     /gene="def"
                     /locus_tag="VFB80_14850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZN35105.1"
                     /translation="MQILTYPHPTLRRVSKPLRRVDAELRQKIRQMFDLMYAAKGIGL
                     AANQVDLPLRLFIVNLAAKPDEGEELVFINPVISLPRGGSEEAEEGCLSLPGVYGNVT
                     RPKQVRLNAYSLAGNEIEADASGLFARCVQHELDHLGGVLFPDRMSPTARADIQDELN
                     VFETEFNSRRATKAIPSDEEIARQWAELEQRYT"
     gene            2892..2965
                     /locus_tag="VFB80_14855"
     tRNA            2892..2965
                     /locus_tag="VFB80_14855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2926..2928,aa:Thr,seq:cgt)
     gene            3032..4363
                     /locus_tag="VFB80_14860"
     CDS             3032..4363
                     /locus_tag="VFB80_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HZN35106.1"
                     /translation="MAQAAAIDIRDVVLSNSSFGPREIGQLTLAISDEFQQFGLLRDA
                     VSELEAREDRTPAAAVRLGVCQYLLGRYRQAIDTLKSADGGALAHFYIGKAQFALGNF
                     ADAIKSYSAAKSAGYNGDDCALAISEVQRNTKDLKKSLETLDKLSGAVEQTAEYLYQR
                     GSTVAALGGNPTEVIALFERAVEVDGRHPGALFGLAVENDRRGNDDTALQLYQRAAAT
                     FPSNVGTLINLGLLYEDKQQFDRAQLCYQRVLDSFPDHPRARLYSKDASASGNILFDE
                     EAQRKQDRLAAVLNIPVTDFELSVRSRNCLQKMGVRTLGDLTRTTEADLLASKNFGET
                     SLVEIRDMLHSKGLELGQFASESAPKLAEPSLDLAAMSPDEQALLERPISDLNLSVRA
                     RKCMARLGLTLIAELIRKTGDDLLECKNFGVTSLNEVREKLTGLGLKLRGD"
     gene            4398..5516
                     /gene="tgt"
                     /locus_tag="VFB80_14865"
     CDS             4398..5516
                     /gene="tgt"
                     /locus_tag="VFB80_14865"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZN35107.1"
                     /translation="MFRFTLHHVAASAAARRGTLHTPHGPVETPAFMPVGTQATIKGL
                     TVDQVRSTGAEMILGNTYHLALRPGEEVIRDLGGLHAFMGWDGPILTDSGGFQLYSLA
                     QNTKVSEQAAVFRSHIDGQLLELSPERSIAIQEALGSDVAMVLDHVIALPAAGQAIKD
                     ACERTIRWAERCRSAARRSDQAQFAIVQGGLDPELRVWCARQLAALDFPGYAVGGLSV
                     GESPDEMYRVLEIVCPALPADKPRYLMGVGTPRDLLQAIGRGIDLFDCVMPTRNGRNA
                     LAFTDAGPLRLRNSIHQRDERPLEEGCPCLACRHSRAYLRHLFLADEMLGPILLTHHN
                     LTYYQRLLAAARAAIAADGYAEFCATRVSGWSREPQSA"
     gene            5598..6995
                     /locus_tag="VFB80_14870"
     CDS             5598..6995
                     /locus_tag="VFB80_14870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN35108.1"
                     /translation="MKLHFLGANRQVTGSRYCLEVAGGKVMIDCGMFQERKFEGRNWD
                     ACPLPAAGIDALLLTHVHIDHCGLIPKLVKEGYRGPIHTTEPSAGLIEIMLRDSAEIQ
                     VEDLKYKQKRHRKAGRQGPHPYQPLYTEEDVDQTLPLVRPCGYSLPVQVTPQVTARFF
                     DAGHILGSAMIQLDVDEAGRQTRLVFSGDIGQSDKPIIRDPSLLAEADYVVMESTYGD
                     RNHRRVGDTETQLAAVINDTVARGGHVIIPTFAVERAQELIYHLARLVHARSIPQLPV
                     FLDSPMAVDVTAIYLKFQHYCDEALLEMIEQHQPPLRFPTLTMVRTAEESKKLNSLSQ
                     PSVIMASSGMCNAGRIKHHLRNNIEKPEATILFVGHQGEGTLGRQILDGQKRVRIHGQ
                     DYTVRAKVAQIHGFSGHADHDGLLKWITFFKRRPKRVFFTHGEEQVALQFAGEVRAKL
                     GYDTHVPEYREVVEL"
     gene            7043..7579
                     /gene="gmhB"
                     /locus_tag="VFB80_14875"
     CDS             7043..7579
                     /gene="gmhB"
                     /locus_tag="VFB80_14875"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZN35109.1"
                     /translation="MFLDRDGVINEDSGFVTRPEDFVFRPGAIESLAEIQRLGYLLIV
                     VTNQSGIGRRLYTDEDYARVTQHMLAQLASSGVRVDGVYHCPHSPDDGCSCRKPAPGL
                     IQKAAQEHQIDLGRSWLVGDKQIDIAAADRAGIRKTLLIRSPYPVDPTGAKPLFVGDS
                     LAAIMPPLRREVEAGDPA"
     gene            7566..8528
                     /gene="rfaD"
                     /locus_tag="VFB80_14880"
     CDS             7566..8528
                     /gene="rfaD"
                     /locus_tag="VFB80_14880"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HZN35110.1"
                     /translation="MTQLDLNGRTVLITGGAGFIGSNLALHIEQQFPKCRVIVADAFA
                     LGHFQNLRGFRGECLAADIASEADLKQLAGLRFDYLFHQAAISDTTVTDQQLMLRVNT
                     NAFRKLLEIAASLSAPVVYASSAGVYGNSPAPNRVGEGEQPENVYGFSKLMMDHVARE
                     FSRARPELRIVGLRYFNVYGPRESFKGRMSSMILQLGRQLLADQSPRLFKHGEQRRDF
                     VYVEDVVQANLKALSARQSGVYNVGSGRARSFNEVLEILGSKLGVRPEALYIDNPWNF
                     FQTHTEADIETTQSQLGYQPQFSLEAGIERYAPEIRRLAAREAA"
     gene            8525..9928
                     /gene="rfaE1"
                     /locus_tag="VFB80_14885"
     CDS             8525..9928
                     /gene="rfaE1"
                     /locus_tag="VFB80_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HZN35111.1"
                     /translation="MISLRGAAPHIAVVGDLMLDHYLIGGCQRISPEAPVPVVQVERE
                     THSLGGAGNVVANLRALGARVSLASAIGDDTCGGRVLELLQQQGVNSCGVARTAGRIT
                     SQKTRVVVSRHQLVRIDREWAQPIGALEQQALFESLTAGEPPAAVLLSDYAKGVLTPA
                     LCQQIIGWARARSIPVLVDPKGAEGGKYRGATLVTPNRKEAAELTGIATSTDASLLAA
                     GQHLRRDLALEYAIITLSEEGLAVFDGRMSRIRAAAREVFDVSGAGDTVLAALGFGLA
                     SGLTIHEAAELANRAAGIVVGKFGSATVTLDEIEAAGGASAIQSAAEVEQTAERLRGQ
                     GRKIVFTNGCFDILHRGHIEYLRASKACGDVLIIGLNSDASVRRLKGPSRPVVSQEDR
                     ATILADLSAVDYVVIFDEETPYELIRRIRPDVLTKGADYAGRQVVGSDLVSDVRLIPF
                     VAGRSTTGTIDRIQRAA"
     gene            9987..10994
                     /gene="waaF"
                     /locus_tag="VFB80_14890"
     CDS             9987..10994
                     /gene="waaF"
                     /locus_tag="VFB80_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZN35112.1"
                     /translation="MRILVRMPNWLGDAVMATPALHNLLRHYEGAQFVLVGSRVVAEL
                     FAGDRRFAAVIADESGKARPRLAGLWRLARRLQRDHGPFDEGWTFQNGLSSRLLLWTA
                     GARRRIARRHHGWADWLLTDPIRCDLTRHHTEIYNGTVNGGLGTSYPAGPTVLPPIRP
                     HDYLRPTAGLHPGAAYGDAKRWLPERFAETAAALARQYDIVLFAGPDERELAEQVEAL
                     LIAGGVKNATNLGGRTSIRELVARIAGLDLFLANDSGPMHVAGALGVPTVAIFGSTDP
                     RVTCPWSAQHTRIVRRELPCSPCHARTCPLHHHACMREITAEQVLAAARSLATPHRAA
                     G"
     gene            11537..>12784
                     /locus_tag="VFB80_14895"
     CDS             11537..>12784
                     /locus_tag="VFB80_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35113.1"
                     /translation="MFSSGRFAVALVVLVVAPNLVADEPPTPDKKPASPVLVEPDAKL
                     TDIQISVFNSTGNKPLPAFRVIAGVRSGVSDEFEKKTGRPVVNWQPHTLKPGKDGDLI
                     WPLAKAYDEMALRVEADGYQPQVWAWLRKKEGPKHIVFQLADDPGVAGRVLTPEGKPA
                     AGATIALAMAQRDAVLDNGRLRHVDEPPAEKPGDQRRRPLFVKSDAEGRFRLPTEIEP
                     AAVLIVHDAGVSELAYDAWAKAPEVTLQGWGRIEGSILWKDKPGAGEPVSLIIHRDDY
                     GYPGMVAQYAKSKADGDGKFVFDRILPGHVQLSRPLPASGGGETILPGMFIHLKVAAG
                     QPTPAVIGGQGRQLTGKLKGRDSWEDVTLHFHPNAPRVGFGGDDAQWKAFSELKAGPL
                     GPLLFRDKIKPAADGTITIDNVLP"
BASE COUNT         2201 a         4246 c         4233 g         2104 t
ORIGIN      
        1 aacagaaaat cagcgactct tcgacatcga agtcatctcc tccggccacc gctttccgat
       61 gggtatggcc cgcaatcgtg acggcgagct gttcgtcacg gataaccagg ggaactacaa
      121 cccgttcaat gagctgaacc atgtgcggaa gggggcgcat tttgggttca tcaacgcgct
      181 ggagaaggag cagggattta ggccgccgcc gctggcggag ccggcgatca atattccgca
      241 tccctggacg cggagcgtga acgggatttg cttcttggaa acgcccgtag gatgggtcaa
      301 ggacgcggag cgtccgcaga cccacgcggg aacggcgtcg gaggctggag cgccgcgtgg
      361 gtcttcggcc gccgcggcct cgacccaccc tacgatgttc gggcccttgg agggtcagct
      421 cgtcggctgc gaatacgaca cgcggcggct gattcggatg tcgcttcaga aagtcggcga
      481 tacgtatcag ggagccgcgt atccgctgag cgtgccgccg gcggaggttg agaagggcct
      541 gctcggaccg atcgtctgtg cgatcaagcc cacgacggcg gagctatacg tcggcgagat
      601 tcacgacagc ggctggggcg cgggaaacaa catcggccag atcgtgaaga tcaagatcga
      661 gccggagaag ctgccctgcg gcatcgccga ggtccgcgcg acctccggcg gctttgccat
      721 cgacttcatg cagccggtgg atcgggcgaa ggcggccgat ctggccagct acgctatcga
      781 atcgtatcgc cgcgaatcga cgcccgcgta cggcggcccg gacatcgacc ggcgaatcga
      841 gaaaatcggc tctgcggaag tcgcggccga cggcaaacgc gtgatgctca cgctgccgaa
      901 cctgcgggcc ggctttgtgt atgagctgaa gctcaagaat ctcgcgccgg ccggcagtgt
      961 gttcttcccc gacgaggccc actacacgct gcacaagatt ccgtagccga cgctgccagc
     1021 gtcgcctacg gctatcccag ccgctcgcct tcgcggatgt ggtacccgtg caggaagtcg
     1081 gcgacgggca tgacccgctt gccggcgggc tgcacgttga tcagcgacag gccgccgtcg
     1141 ccggtagcca cgatcagctg ctggcggtcg ctgacgacga cctcgcccgg ctgatggacg
     1201 ggcgcggagc tatcgaccat ggctgcggcg cggatgccca ggccgagaat gctgacgcgg
     1261 tcgatgacga tccgcagcgg ctcgtggccg gggcgcgacc agaaggtgaa cgtgccgggc
     1321 cagggcttga gcgcccgcac ctggttgcgg atctgctcgg ccgtgcgctg ccaatcgatc
     1381 agaccgtgct ccttcttcag ccgcggggcc ttcgtcgcca gctgcgggtc ttgcggcgta
     1441 ccgatggggc tggcgcggtc ccacttgccg agcaaatcga tcgcttcgtg gaccgcttcg
     1501 atgcccaggt gggccaggcg gtcctcgagc gctagctgcg tctcgtcgga ggagatcagc
     1561 gtcgcccgta cggccaggat cgggccgccg tccagccgcg gcgtcatgtg gatcacgctg
     1621 acgcccgtct cggattcgcc gtgcaacaag gcccactgga tcggcgctgc cccgcgatac
     1681 ttgggcagca gcgaggcgtg cacattgatg ccgcccaagc gtgcgatacc gagcgcttcg
     1741 gccgagagaa tctgcccata gtcgcagacc acgagcaagt cgggctggag cgagcggatc
     1801 agaatcagcc cctcctcgct gttgacgctc tctggcgcat gcacggtcag cccgcgctgc
     1861 tcggccaggt cgcgcatcgg attgggcggc gctttctcgc gccccttcgc cggcggcgtc
     1921 ggccgcgtaa tcagcgcgag aatctcatgg cgcgaatcca ccaacgactg aaacagcggc
     1981 accgcaaatg ggcccgtacc gagaacgaca attcgcatgt caatttggga ttggggattt
     2041 gggatttggg atggggctga aatcccaaat catacatccc aaattcatgt gtatcgttgc
     2101 tccagctcag cccattgccg ggcgatttcc tcgtcgctgg ggatcgcctt ggtcgcccgc
     2161 cggctgttga attcggtctc gaagacgttc agctcgtcct ggatgtcggc gcgggcggtg
     2221 gggctcatgc ggtcgggaaa gagcacgccg cccaagtggt cgagttcgtg ctgcacgcag
     2281 cgggcaaaga ggccgctggc gtcggcctcg atttcgttgc ccgcgagcga gtaggcattg
     2341 agccgcacct gcttggggcg ggtgacgttg ccgtaaacgc ccggcagact gaggcagcct
     2401 tcttccgcct cctccgatcc gccgcggggg agcgaaatca ccgggttgat gaagaccagc
     2461 tcctccccct cgtcgggctt ggcggcgagg ttcacgatga acagccgcag gggcagatcg
     2521 acctggttgg ccgccagtcc gatgcctttg gcggcgtaca tcaggtcgaa catctggcgg
     2581 attttctggc gcagctcggc atcgacgcgg cgcagcggtt tgctcacgcg gcggagcgtc
     2641 gggtgaggat aggtgagaat ctgcaagcgg agggttcagg gctgggggtt cggggttcag
     2701 agttcaggaa tctgcgcggc gaaccgctga ggaattatag gtgtcgagca ggacgagccg
     2761 taggcgggcc gcagctctat cggcttgccg cgaatcgccg gaagtcctga cggcaccagc
     2821 ccttgacgca aattgaggcc gtacctaggc ttacaggatt acaggctgtc cggccggttt
     2881 cgccgaaaaa tgccagcgta gcttcaatcg gcagagcacc gcattcgtaa tgcgggggtt
     2941 gtgggttcga ctcccaccgc tggctctgcg taggagcgcc ggcaggaacc gtggcggcgg
     3001 cccaggcagc ggatttcgga gtcataccca gatggcgcaa gcggcagcga tcgatattcg
     3061 agacgtcgtt ctctccaaca gctcgtttgg cccccgcgag attggccagt tgacgctggc
     3121 gatctcggac gagttccagc aattcggcct gctccgcgac gccgtgagcg agctggaggc
     3181 ccgcgaggat cgcacgcccg ccgcggcggt ccgcctggga gtgtgccagt atctgctggg
     3241 ccgctaccgg caggcgatcg acacgctcaa gtcggccgac ggcggcgcct tggcccactt
     3301 ctacatcggc aaggcccagt tcgccctggg caacttcgcc gacgcgatca agtcgtacag
     3361 cgccgcgaag agtgcgggct ataacggtga cgactgcgcc ctggcgatct ctgaggtcca
     3421 gcggaacacg aaggacctca agaagtcgct ggagacgctc gacaagctct ccggcgcggt
     3481 cgagcagacc gcggaatacc tctatcagcg cggctcgacc gtcgcggccc tgggcggcaa
     3541 tccgacggaa gtgatcgccc tgttcgagcg ggccgtcgag gtcgatggcc ggcacccggg
     3601 cgctctgttc ggcctggccg tcgagaacga ccgccgcggc aacgacgaca cggcgctgca
     3661 gctctatcag cgggccgccg ccacgtttcc ctccaacgtc ggcacgctca tcaacctggg
     3721 cctgctctac gaggacaagc agcagttcga ccgggcccag ctctgttacc agcgcgtgct
     3781 cgattcgttc cccgatcatc cgcgggcccg gctctattcg aaggacgcct cggcctcggg
     3841 caacatcctg ttcgacgagg aggcccagcg gaagcaggac cgcctcgcgg ccgtgctcaa
     3901 cattccggtc accgatttcg agctctcggt ccgcagccgc aactgcctgc agaagatggg
     3961 cgtgcggacg ctcggcgacc tgacacgcac gaccgaagcg gacctgctcg ccagcaagaa
     4021 ttttggcgag acttcgctgg tcgagatccg agacatgctg cacagcaagg ggctggagct
     4081 ggggcaattc gcgagcgaat ccgcgccgaa gctggccgag ccgtcgctgg acctggccgc
     4141 gatgtcgccc gatgagcagg ccctcctgga gcggccgatc tccgacctca acctgtcggt
     4201 gcgggcccgc aagtgcatgg cccgcctcgg cctgacgctg atcgccgagc tgatccgcaa
     4261 gaccggcgac gacctcttgg agtgcaagaa cttcggcgtc accagtctca acgaagtccg
     4321 cgagaagctg accggcctgg ggctgaagtt acgcggagat tgaggacagg tgtcgggagt
     4381 caggggtcag gagtcaggtg ttccgcttca ccctgcacca cgtcgccgct agcgccgccg
     4441 cgcgccgcgg cacccttcac acgccgcatg gccccgtcga aacaccggcg ttcatgccgg
     4501 tcggcacgca ggccacgatc aagggcctga cagtcgacca ggttcgctcg accggcgcgg
     4561 agatgatcct gggcaatacg tatcacctcg cgctgcggcc gggcgaggag gtgatccgcg
     4621 acctgggcgg cctgcacgcc ttcatgggct gggacggacc gatcctcacc gacagcggcg
     4681 gctttcagct ctacagcctg gcccagaaca cgaaagtcag cgagcaggca gccgtctttc
     4741 gctctcacat cgacggccag ctgctggaac tctcgcccga gcggtcgatc gccattcagg
     4801 aggccctcgg cagcgacgtg gccatggtgc tcgaccacgt catcgcgctg ccggccgccg
     4861 gccaggccat caaggacgcc tgcgagcgga ccatccgctg ggccgagcgc tgccgctcgg
     4921 cggcgaggcg cagcgaccag gcccagttcg cgatcgtgca gggcggcctc gatcccgagc
     4981 tgcgcgtctg gtgcgcccgc cagctcgccg cgctcgattt ccccggctac gccgtcggcg
     5041 gtctcagcgt cggcgaatcg ccggacgaaa tgtaccgcgt gctcgagatc gtttgccccg
     5101 cgctgccggc cgataagccg cggtacctca tgggcgtcgg cacgccgcgg gatttgctcc
     5161 aggcgatcgg ccgcggcatc gacctgttcg actgcgtgat gcccacccgc aacggccgca
     5221 acgccctggc cttcaccgac gccggccccc tgcggctgcg gaacagcatt caccagcgcg
     5281 acgagcggcc gctggaggaa ggctgcccct gcctggcctg ccgccacagc cgagcctacc
     5341 tccgccacct gttcctcgcc gatgagatgc tggggccgat cctgctcacg caccacaacc
     5401 tcacctacta ccagcggctg ctcgccgcgg cccgtgcggc gattgctgcc gatggctatg
     5461 ccgagttctg cgcgacgcga gtgtccggct ggagccgtga gccgcagtcc gcgtagtcaa
     5521 ctgcggccgg cctgagtaca aagggagacg gaccttccac tttagggccg ccggcaatcg
     5581 gccgcggcga aacaagcatg aagcttcact tcctgggcgc gaatcggcag gtcaccggca
     5641 gccgctattg cctggaggtc gccggaggga aggtgatgat cgattgcggg atgttccagg
     5701 agcggaagtt cgagggccgc aactgggacg cctgcccgct gcccgcggcc ggcatcgacg
     5761 ccctgctgct gacgcacgtc catatcgacc actgcgggct gatccccaag ctggtcaagg
     5821 agggctatcg cggcccgatt catacgacgg agccgtcggc cgggctgatc gagatcatgc
     5881 tccgcgactc ggccgaaatt caggtcgagg acctgaaata caagcagaag cggcaccgca
     5941 aggccggccg ccagggcccg catccctatc agccgctcta caccgaggag gacgtcgatc
     6001 aaacgctgcc gctggtgcgg ccctgcggct attccctgcc ggtgcaagtg acgccgcagg
     6061 tgacggcccg gttcttcgac gccgggcaca tcctgggctc ggccatgatt cagctcgacg
     6121 tggacgaagc cggccggcag acgcgcctcg ttttctccgg cgacatcggc cagagcgaca
     6181 agccgatcat ccgcgatccg agcctgctcg ccgaggccga ctacgtcgtg atggaatcga
     6241 cctatggcga tcgcaaccac cgtcgcgtcg gcgacaccga gacgcaactc gccgcggtca
     6301 tcaacgacac ggtcgcccgc ggcggccacg tgatcatccc cactttcgcc gtcgagcggg
     6361 cccaggagct gatttatcac ctcgcccggc tggtccacgc ccgcagcatc ccgcagctgc
     6421 cggtctttct cgacagcccg atggcggtcg atgtcacggc gatctatctc aagttccagc
     6481 actactgcga cgaggccctg ctggagatga tcgagcagca ccagccgccg ctgcggtttc
     6541 ccacgctcac gatggtccgc acggccgagg aatcgaagaa gctgaatagc ctgtcgcagc
     6601 cgagcgtgat catggccagc tccggcatgt gcaacgccgg acggatcaag caccacctgc
     6661 ggaacaacat cgagaagccg gaggcgacga tcctgttcgt cggccaccag ggcgagggca
     6721 ccctcggccg gcagatcctc gacggccaaa agcgggtgcg gattcacggc caggactata
     6781 cggtgcgggc caaggtcgcg cagatccacg gcttctcggg gcacgccgac cacgacggcc
     6841 tcttgaagtg gatcacgttc ttcaagcgcc ggcccaagcg ggtcttcttt acccacggcg
     6901 aagagcaggt cgccctccag ttcgcgggcg aggtccgcgc caagctgggc tatgacacgc
     6961 acgtgccgga gtaccgggaa gtggtggaac tgtgagccag gggccagggg ccaggggcca
     7021 ggggccagtc ggcaggcggg ccgtgttcct cgatcgcgac ggcgtgatca acgaggattc
     7081 gggattcgtc acacggccgg aggatttcgt cttccgcccg ggcgcgattg agtcgctggc
     7141 cgagattcag cggctcggct atttgctcat cgtcgtgacc aatcaatcgg ggatcggccg
     7201 ccgcctctac accgacgagg actacgcccg cgtgacgcag cacatgctgg cgcagcttgc
     7261 tagcagcggc gtgcgggtgg acggcgtcta tcactgcccg cactcgcccg atgacggctg
     7321 ctcctgccgc aagcccgccc cgggcctgat tcaaaaggcc gcccaggagc accagatcga
     7381 cctgggccgc tcgtggctcg tcggcgacaa gcagatcgac atcgcagccg ccgaccgcgc
     7441 gggcattcgc aagaccttgc ttattcgcag cccgtatccg gtcgatccaa cgggcgctaa
     7501 accgctcttt gtgggcgatt ccctggccgc tatcatgccc ccgctccgca gggaggtgga
     7561 ggcaggtgac ccagcttgat ctcaacggcc ggaccgtgct gataaccggc ggcgcgggct
     7621 tcatcggctc gaacctggct ctgcacatcg agcagcagtt tcccaagtgc cgcgtgatcg
     7681 tggccgacgc cttcgccctg ggccacttcc agaacctccg cggctttcgc ggcgaatgcc
     7741 tggcggccga catcgccagc gaggccgacc tcaagcagct ggccggtctg cgtttcgact
     7801 acttgtttca ccaggcggcg atcagcgaca cgacggtaac cgaccagcag ctcatgctgc
     7861 gggtcaacac caacgccttc cgcaagctgc tggagattgc ggcgtcgctc tcggccccgg
     7921 tcgtttatgc ctccagtgcc ggcgtctatg gcaattcgcc cgcccccaac cgcgtcggcg
     7981 aaggcgagca gccggaaaac gtctacggct tctcgaagct gatgatggac cacgtggccc
     8041 gcgagttttc gcgggcccgg ccggagctgc ggatcgtggg actgcggtac ttcaacgttt
     8101 acggcccgcg ggagtcattc aagggccgca tgagctcgat gatcctccag ctcggtcggc
     8161 agctgctcgc cgaccagtcg ccccggctat tcaagcacgg cgagcagcgc cgtgacttcg
     8221 tctatgtcga ggacgtcgtg caggcgaact tgaaggccct ctcggcccgc cagagcggcg
     8281 tttacaacgt cggcagcggc cgcgctcgct cgttcaacga agtgctcgag atcctcggta
     8341 gcaagctcgg cgtgcgaccg gaggccctgt acatcgacaa tccctggaac ttctttcaga
     8401 cgcataccga ggccgacatc gagacgacac agtcgcagct gggttatcag ccgcagtttt
     8461 cgctcgaagc cggcatcgag cggtacgctc ccgagatccg ccgcctggcg gcgcgggagg
     8521 cggcatgatc tcgctgcgcg gcgcggcgcc gcacattgcc gtcgtcggcg acctgatgct
     8581 cgatcactac ctgatcggcg gctgccagcg gatcagcccc gaagcgccgg tgcccgtcgt
     8641 gcaggtcgag cgggaaacgc actcgctggg cggcgcgggc aatgtggtgg ccaatttgcg
     8701 ggcgctcggc gcgagagttt ccctcgcctc cgcgatcggc gacgatacct gcggcggccg
     8761 cgtgctggaa ctgcttcagc aacagggcgt caactcctgc ggtgtcgctc gcacggcggg
     8821 ccgcattacg tctcaaaaga cgcgcgtcgt cgtttcgcgg catcaactcg tgcggatcga
     8881 ccgcgagtgg gcgcagccga tcggcgcctt ggagcagcag gcgctgttcg agtcgcttac
     8941 cgcgggcgag ccgcccgccg cggtattgct gtcggactat gccaagggcg tgctgacgcc
     9001 ggcgctctgc cagcagatca tcggctgggc ccgcgcgcga tcgatccccg tgctggtcga
     9061 tcccaaaggc gcggagggcg gcaagtaccg cggggcgacg ctcgtgacgc ccaatcgcaa
     9121 ggaagccgcc gagctgaccg gcatcgcgac tagcaccgac gcgagcctgc tggcggcggg
     9181 ccagcacttg cgccgcgacc tggcgctcga atacgccatc atcacgctct ccgaagaagg
     9241 cctggcggtc ttcgacggcc ggatgagccg gattcgcgcc gccgcccgcg aggtgttcga
     9301 cgtcagcggc gcgggcgaca cggtgctggc cgcgctcggc ttcgggctgg ccagcggttt
     9361 gaccatccat gaggcggcgg agctggccaa ccgggcagcc gggatcgtcg tcggcaagtt
     9421 cggcagcgcg accgtcacgc tcgacgagat cgaagccgcg ggcggcgcat ccgccatcca
     9481 gtccgccgcg gaagtcgagc agactgccga gcggctgcgc ggccagggac gaaaaatcgt
     9541 cttcaccaac ggctgcttcg acatcctcca tcgcggccac atcgaatatc tgcgggcgtc
     9601 gaaggcctgc ggcgacgtgc tgatcatcgg gctgaattcc gacgcgagcg tccgccgctt
     9661 gaaaggcccg tcgcggcccg tcgtctcgca ggaggaccgc gcaacaattc tggccgacct
     9721 gtcggcggtc gattacgtcg tgatcttcga cgaggagacg ccctacgagc tcatccgccg
     9781 cattcgcccc gacgtgctca cgaagggagc cgactatgcc ggccgccagg tcgtcggcag
     9841 cgacctggtg agcgacgtgc ggctgattcc gttcgtcgcc ggccgctcga cgacgggcac
     9901 aatcgaccgc atacaacggg ccgcgtagcc cgccactccg tggcgggcga cacgcgacgg
     9961 agtcgcgtgc tacaaaccag caaaacatgc gaatcctggt gcgaatgccg aactggctgg
    10021 gggacgccgt gatggcgacg ccggcgctgc acaacttgct gcggcactat gaaggcgcgc
    10081 agttcgtgct cgtcggctcg cgcgtcgtgg cggagctgtt cgcaggggac aggcgctttg
    10141 ccgccgtgat tgccgatgaa agcggcaagg cgcggccgcg tctggccggc ttgtggcgac
    10201 tggcccgacg cctccagcgc gaccacggcc cgttcgacga aggctggacg tttcagaacg
    10261 ggctttcctc gcggctgctc ctctggacgg ccggcgcgcg gcggcgaatc gcacgccgcc
    10321 atcatggctg ggccgattgg ttgctcaccg acccgatccg ctgtgacctg acgcggcacc
    10381 acaccgaaat ctataacggc accgtcaacg gcggcttagg cacaagctat cccgccggcc
    10441 ctacggtgct gccgccgatc cggccgcacg actacctgcg gccgaccgcc ggcctgcatc
    10501 cgggcgcggc ctatggcgac gccaagcgct ggctgcccga gcggtttgcc gaaacggcgg
    10561 cggccctcgc gcggcagtac gacatcgtgc tcttcgccgg gccggacgag cgcgagctgg
    10621 ccgagcaggt tgaagcgctg ctgatcgccg gcggcgtgaa gaacgcgacc aatctgggcg
    10681 ggcggacttc gattcgcgag ctggtcgcgc ggatcgccgg cctggatttg ttcctggcca
    10741 acgactccgg tccgatgcac gttgccggcg cgctgggcgt gccgaccgtc gccatcttcg
    10801 gctcgaccga tccgcgcgtc acctgcccct ggtccgccca gcacacccgc atcgtccgcc
    10861 gcgagctgcc ctgctcccct tgccacgccc gcacctgccc actgcaccac cacgcctgca
    10921 tgcgcgagat caccgccgag caggtattgg ccgcagcccg ctcgctcgca accccgcacc
    10981 gggccgcggg ttaagttcag gcgccgggcg aattgccagt ttccggtcca gaaagccgat
    11041 ccgcgcgccc attttcttgg aaatagtcgc aagcctcgtc cgcacctaga cttgcgaggc
    11101 gaccatcggc ccatttttga tcgcccgatg gcacaaactt caggaccctg tgatataatc
    11161 tgtccactta agaaacgtac gattcaagcg aattcaaggg agcggtgcca ggcactgctc
    11221 gctcgccgtg cgcgcatcga aaggcggggt gccgaatcgg ttgctgggta gttggagagg
    11281 tgccgagaga ggtaaggata attccccgcg aacccttcaa acacttcaaa cactaatgcc
    11341 gtttctagcg ctcagaatcg ccgcaacctt agccgcagca aacagttgcg cgccgccatt
    11401 cgcttcagcc actgcgatcg ccagtcgctg cacatgggaa ttatccttac ctcgattcca
    11461 ggtgttttgg cgagttgaat ccgacacgaa cgttggcccg tcttacggct agaccatgcg
    11521 aaataggagc aaggccatgt tcagttctgg tcgcttcgcc gtagccctgg tggtgctcgt
    11581 ggtcgctccg aatctggttg cggatgaacc gccgacgccg gacaagaagc cggcttctcc
    11641 tgtccttgtc gagccggacg caaagctcac cgacatccag atttccgtct tcaactccac
    11701 gggcaataag ccgctcccgg cgtttcgtgt gatcgcgggc gtccgctccg gagtttcgga
    11761 cgagtttgaa aagaagactg gccggccggt cgtcaactgg cagccgcaca cgctcaagcc
    11821 gggcaaggac ggcgacctga tttggccgct ggcgaaggct tacgacgaaa tggccctgcg
    11881 ggtcgaggcg gacggctatc agccgcaggt ttgggcctgg cttcgcaaga aagaagggcc
    11941 caagcacatc gtgtttcagt tggctgacga tccgggcgtg gcgggccgcg tgctgacgcc
    12001 ggagggcaag ccggcggccg gcgcgaccat cgccctggcg atggcccagc gcgacgccgt
    12061 gctcgacaac ggacgcctgc ggcacgtgga tgagccgccg gccgagaagc cgggcgatca
    12121 gcggcggcga ccgctatttg tgaagtcgga cgccgaggga cggtttcgcc tgccgaccga
    12181 aatcgagccg gccgccgtgc tgatcgtcca cgacgccgga gttagtgaat tagcgtacga
    12241 cgcctgggcg aaagcgccgg aagtgacgct gcaaggttgg ggccgcatcg aaggaagcat
    12301 cttgtggaag gacaagcccg gcgccggtga gcccgtgagc ctgattattc atcgcgacga
    12361 ctacggttat ccgggcatgg tcgcacagta cgccaaaagc aaggcggacg gcgacggcaa
    12421 gttcgtgttc gaccggattt tgcccggcca cgtgcagctt tcgcggccgc tgccggccag
    12481 tggcggcggc gagacgattt tgcccggcat gttcatccac ttgaaggtcg cggcgggcca
    12541 gccgacgccg gccgtcattg ggggccaggg ccggcagctt accggcaagc tgaagggacg
    12601 cgattcctgg gaagacgtca cgctgcattt tcatcccaac gcgccgcggg tcggcttcgg
    12661 cggcgacgat gcgcagtgga aggccttttc ggagctcaag gccggcccgc tgggcccgct
    12721 gctgttccgc gacaagatca agccggctgc cgacggcacg attacgatcg acaacgtgct
    12781 gccg
//
LOCUS       DASRJB010000570         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2127986, whole genome shotgun sequence.
ACCESSION   DASRJB010000570 DASRJB010000000
VERSION     DASRJB010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127986"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..738
                     /locus_tag="VFB80_14900"
     CDS             109..738
                     /locus_tag="VFB80_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HZN35114.1"
                     /translation="MARILITSGPTRQYLDPVRYLTNASSGRMGRELASAALELGHQV
                     IVVSGPVSLEYPAGAQVRPVVSTEELLAACQELFPGCDGAIGAAAPCDYRPLRVEEHK
                     IAKTGQPLVLNLEETPDVMATLGASKRADQWVVGFALETDDQRFRALTKLIKKSCDLI
                     VLNGPAAMDAADNEVEVLDHSGAVVAAARGSKQEVAREILRTIQQRLID"
     gene            complement(763..2160)
                     /locus_tag="VFB80_14905"
     CDS             complement(763..2160)
                     /locus_tag="VFB80_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN35115.1"
                     /translation="MSAWRRMFASGLRGMLVAGVWLSFFPAAVALAAPPSTSELRAWL
                     AGLDADDYAQRENASRRLIEAGEAAIDLLAEGVGSPSAEAAWRASSALEQIALHGDES
                     ALARVSEALARLSQQGRPGLAHLAQELKAKQAQLKRERAAARIRGFGGRLSGSEYSEV
                     FGGFFGGGMPAIVEVIDEVVAEKVAEDIKVLVEAGEPVADDGKPLEISPDLKAFGDGL
                     KLAAAGMGKAAEPNEKDPPPPPDAPPPEPLAPPAPPAADEPPAIAVAAVDPFAEVFIA
                     DAVGIDLGFAVEGDVEAQESLAIGDDWRGGDAGLAALRDLPSVVSIQISGKQLTDAAL
                     PHIAALPKLASLELQGTAFSAAGLWKFRQQRPAARIYARGDAMLGVNADTEGPCVLTS
                     IYYGSGAFDAGLVQGDEIVSVDGHKVADFSDLTIAVFTHQPGEKLQVEFKRGDELKKV
                     SILLKDRKDVDRSRR"
BASE COUNT          442 a          929 c          896 g          454 t
ORIGIN      
        1 caccaggccg aggagacgct gcacgccttc tccggcgcac gctccgcgtt ctggaagtaa
       61 acgtgtcaac tgctgcggga gcaagccgct cccgctacga tattgcccat ggcccgcatc
      121 ctgattacct ccggaccgac gcggcagtac ctcgatccgg tccgctatct cacgaatgcc
      181 tccagcggcc gcatggggcg ggaattggcc tcggcggccc tggagctcgg acaccaggtg
      241 atcgtggtca gcgggccggt gagcctcgag tatcccgcgg gggcacaggt gcggccggtc
      301 gtttcgaccg aggagctgct ggcggcctgc caggagctgt ttcccggctg cgacggcgcg
      361 atcggggcgg cggccccctg cgattaccgg ccgctgcggg tcgaagagca caagatcgcc
      421 aagaccgggc agccgctggt gctgaacctg gaggagacgc ccgacgtaat ggcgacgctg
      481 ggcgcgtcga agcgggccga ccagtgggtg gtgggttttg ccctggaaac cgacgaccag
      541 cggttccgcg cgctcaccaa gctgatcaaa aagagctgcg acctgatcgt gctcaacggg
      601 ccggccgcga tggacgccgc cgacaacgaa gtggaggtgc tcgaccacag cggggccgtc
      661 gtcgccgctg cccgtggcag caagcaggag gtggcgcggg aaatcctgcg gacgatccag
      721 cagcggctga tcgactgagc gcgaccactg gtcgcggctt tgtcaacgtc tgctgcgatc
      781 cacgtccttg cggtccttga gcaggatgct gaccttcttc agctcgtccc cgcgcttgaa
      841 ttcgacttgc agcttctcgc cgggctggtg cgtgaaaacg gcaatcgtga ggtcgctgaa
      901 atcggcgacc ttgtggccgt ccaccgagac gatctcgtcg ccttgaacca ggccggcgtc
      961 gaacgccccg gagccgtagt agatgctggt caggacgcac ggcccttcgg tgtcggcatt
     1021 gacgccgagc atcgcgtcgc cgcgggcgta gattcgcgcc gcaggccgct gctggcggaa
     1081 cttccacaag cccgcggccg agaacgccgt gccttgcagc tccaggctgg ccagcttcgg
     1141 cagggccgcg atgtgcggca gggcggcatc ggtgagttgc ttcccgctga tctggatcga
     1201 gacgacgctg ggcaaatcgc ggagcgcggc caggcccgca tcgccgcccc gccagtcgtc
     1261 gccaatggcg agggactcct gcgcctcgac gtcgccttca acggcaaagc ccaggtcgat
     1321 gccgacggcg tcggcgatga acacctcggc gaacggatcg acagcggcca cggcgatcgc
     1381 cggcggctca tcggctgcag gcggagcagg aggagccaag ggttcaggcg gcggcgcgtc
     1441 gggtggcggc ggcgggtcct tttcgttcgg ctccgcggcc ttgcccatgc cggcggccgc
     1501 gagcttcagt ccgtcgccaa acgccttcag gtccggcgag atttcgagcg gcttgccgtc
     1561 gtcggccacc ggctcgcccg cctcgaccag caccttgatg tcttccgcga ccttctcggc
     1621 cacgacttcg tcgatgactt ccacgatcgc gggcatcccg ccgccaaaga agccgccaaa
     1681 gacctccgaa tattccgacc cgctcagccg accgccgaaa ccgcggattc tcgcggcggc
     1741 ccgctcgcgt ttgagttgcg cctgtttggc cttcagctcc tgagccagat gggccaggcc
     1801 cgggcggccc tgctggctca gccgcgcgag cgcctcgctg acgcgggcca gtgctgattc
     1861 gtctccatgc agggcgatct gttccagggc cgagctggcc cgccaggccg cctcggcgct
     1921 ggggctgccc acgccttcgg ccaggaggtc gatcgctgct tcgcccgctt cgatcaaacg
     1981 ccggctggcg ttctcgcgct gggcgtagtc gtcggcgtcg agccccgcga gccaggcgcg
     2041 cagctcgctc gtggaaggcg gcgctgccag cgccactgcc gcgggaaaga aactgagcca
     2101 gactcccgca acgagcatcc cgcgcagccc gctggcaaac atcctgcgcc acgcgctcat
     2161 gactcgtctc cgcatccgcc cccaagcata accctggtta catactatgg gtcaccgata
     2221 gccgttgtcg agagttaagc caaatgcctt tagctgccgc ggccaaggac tggccccctg
     2281 gttgggcgag ggcgaacgtc ggttttttgc ttattccgtg tcttaaccgt tgatgcacca
     2341 gcgacttgcg gcgcgaccgt cggcggcccg ttaagtacta cttataaggg tttgaggact
     2401 ggcttcgtcg cgcctcctcg tgggcggcaa cgtgcggctc aaggccgcac tcgcaccggc
     2461 cttcacggcc gatccgggac gaggccagct ttccttcgat tcgccaaacg tggatgagga
     2521 cacgacgatc tcgtgcgaaa cgacgagccc gcccgcgacc cgccaggcgc cacggaatcg
     2581 caatggacct cacggcgacg agaattgtaa ctatccgtcc agtatatgtc aagcgcaaca
     2641 caacagattg tgttccgcct ccgcccgacc acaacctgta gtgggtcgcg ccttttagcg
     2701 gcccctgaaa ggagggcggc c
//
LOCUS       DASRJB010000571         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_726856, whole genome shotgun sequence.
ACCESSION   DASRJB010000571 DASRJB010000000
VERSION     DASRJB010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726856"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFB80_14910"
     CDS             complement(<1..588)
                     /locus_tag="VFB80_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN35116.1"
                     /translation="MWARNLLFLAICLLGAGLVARELLRHDRVAEPRTFSAARVAATA
                     EGTSAAGLADEWSQTLSRLNAEFREHWKAQSLEAAPPADDLALARRLSLALVGSVPSL
                     EEIRALEAVSPEARLEWWTSHLLEDRRFADYWAERLARIYVGNEMGPFILFRRRRFTL
                     WLSEQLHDGTPYDEIVRRLISEEGIWTGKPATNFIT"
     gene            730..2148
                     /locus_tag="VFB80_14915"
     CDS             730..2148
                     /locus_tag="VFB80_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35117.1"
                     /translation="MTFGTSQLDPAQGSPPLDVALDVLEVESPPQTEVVAEAIETRDE
                     VVAEAVEPPPRTWPRSWPMIVWYGLCWSLEWLFGLASLLATLAVLAAIPLVNFVSLGY
                     LLEASGRVAKSGKLRDGFIDIPKFARIGSIALGTWLWLLLPRLISSLATDAWLIDPGG
                     WRARAWQIGLIVCTAVVISHILLAWFSGGRLRHFFWPLLAPFQLTGRLLFGGIVSPIT
                     RPLVRSIWPALADDIYAMQPLSEWFPPAILVAAIRRGRMYQSARDAVWDFVVSLQLPH
                     YFWLGLRGFAGAIAWLLLPSLMLMVGTSGRGPLTILIGYLGALGLAFVLLYLPFLQTH
                     FACQNRLAALFEWSAVRAQFRRAPIAYWLALAITLLFALPLYLLKIEPPPRELWWTVT
                     IFFILFIYPARLLTGWAVGRGLHHAKPRFFLFRWMARLAAVPIVLFYVFILFFTQYTS
                     FLGPASILEQHAFLVPVPFLAM"
     gene            complement(2161..2604)
                     /locus_tag="VFB80_14920"
     CDS             complement(2161..2604)
                     /locus_tag="VFB80_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35118.1"
                     /translation="MSLSRNGRRRLCLAGYVGVLAAISLVAGCLGGNAASVTGAVTLD
                     GQPLTTGDVSFYPDGSPGAAAYGQINAEGKYTLSTGTAAGLSPGKYVAVVVATKPLPP
                     PKDPYAPEVPPERITPAKYATVSTSDLHVEVKPGANDLPLSLKSK"
     gene            complement(2623..3552)
                     /locus_tag="VFB80_14925"
     CDS             complement(2623..3552)
                     /locus_tag="VFB80_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35119.1"
                     /translation="MSEPAARRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMA
                     CSNNLKQFGIAIHNYHDVHLTLPISISYARENPRPAPQVNGKGWILSILPQLEQPALF
                     TQFEPGFNGMFSATGGIQMPACRTAIRTRIKVIECPSDGKSKRIITNAAQWGGIEVAV
                     TNYKGVIGDTRMGGATSIHQGTEPDTHNTIGNNGLFYRNSYQEPNRLATITDGTANTF
                     MVGEDVPDQNIHSAAYFANGDYASCHAPLNYFPKPPTPGSWWNVISFRSLHPSGAHFC
                     LADGSVHFVSQTIDYTLYRRLSSKNGGENAQLP"
     gene            complement(3674..4846)
                     /locus_tag="VFB80_14930"
     CDS             complement(3674..4846)
                     /locus_tag="VFB80_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35120.1"
                     /translation="MTRRLSIGIAVCLIGSGAIATAQSFGPQPGQAQQPQQRQAYGPQ
                     SGPAQNPPPQPVGGNPIRPVAGQQPAGQAPPPGNLPPANIAPSPQPPQWALTMSPEEA
                     DWINRVLAYWENHSNKIKTFSCNFQCWEYNAHAPLDVAQRYSEGVIKYAQPDKGLFRV
                     EKLTFYEPPPRPNPAAQPAAAPAPPEKPKYVVQDKSLGEHWVCDGKQVFQFEARTRRV
                     IVLPLPVEMQGKAIADGPLPFLFGAKAETIKARYWVRRLPQTGNGKYWLEAVPKSRDD
                     ARNFKMVHIVLEEKDFLPDMLTVFEPNSSEVTPYRKTYVFPAASRQTTTDKMAVGDLL
                     KALDIFRREFYEPTVPSGWKKEVQSDVVSVINPPPGARPPQEAQRPPQKSPLPLPR"
BASE COUNT          732 a         1581 c         1632 g          936 t
ORIGIN      
        1 cgtgatgaag ttggtcgccg gcttgccggt ccagatgcct tcttccgaga ttagccggcg
       61 aacgatttcg tcgtagggcg tgccgtcatg gagttgctcg ctcagccaga gcgtgaagcg
      121 gcggcggcgg aagaggatga acggccccat ctcgttgccg acatagatcc gcgccagccg
      181 ctcggcccag tagtcggcaa agcgacggtc ctcgagcagg tggctggtcc accattccag
      241 ccgggcctcg ggcgaaacgg cctccagcgc gcgaatctct tccagcgacg gcacgctgcc
      301 gaccagcgcc agcgacaacc ggcgggccag cgccaggtcg tcggccggcg gcgcggcttc
      361 caggctctgc gccttccagt gctcccggaa ctcggcgttt agccgggaca gcgtctgtga
      421 ccattcgtcg gccaggccag cagccgaagt accctcagcc gtggccgcga cgcgggccgc
      481 gctgaacgtg cggggctctg cgacccggtc gtgccgcagc aattccctgg caacaaggcc
      541 ggcgccgagc aggcaaatcg ctaggaacag caggttgcgg gcccacattg ttgctaggcc
      601 agtttgttgc taggccaggt tgttgccagg ccagttgcgg ctggctcggt ttggattgaa
      661 acgcccggcc acggacggta ggtattactc caaggataac ttccgggttt cgctgcgggc
      721 acgcggaaaa tgacgttcgg gaccagccag ctcgatccgg cgcaagggtc gccgccactg
      781 gacgtcgccc tggacgttct ggaggtcgag tccccgccgc aaacggaggt cgttgcggaa
      841 gccatcgaga cccgtgacga agtcgtcgcc gaagcggtcg agccgccgcc gcgcacgtgg
      901 ccgcgcagct ggccgatgat cgtctggtac ggtctgtgct ggagcctgga gtggctgttc
      961 ggcctggcga gcttgctggc gacgctggcc gtgctcgcgg cgatccccct cgtcaacttc
     1021 gtcagcctcg ggtacctgct ggaagccagc ggccgcgtcg ccaaaagcgg caagctccgc
     1081 gatggtttca tcgatattcc caagttcgct cgcatcggca gcatcgccct gggcacgtgg
     1141 ctgtggctgc tcctgccgcg tctgatatcg tcgctggcga cggacgcctg gctgatcgat
     1201 cccggcggct ggcgggcgcg ggcctggcag atcgggctca tcgtctgcac cgcggtggtc
     1261 atcagccaca tcctgctggc ctggttcagc ggcgggcggc tgcggcactt tttctggccg
     1321 ctgctcgcac cgtttcagct gaccgggcgg ttgctgttcg ggggcatcgt gagtccgatc
     1381 acgcggccgc tggtgcggtc gatctggccg gccctggcgg acgacatcta cgcgatgcag
     1441 ccgctgtcgg agtggttccc gccggcgatc ctcgtggcgg cgattcgccg cggccggatg
     1501 taccagtccg cccgcgacgc cgtgtgggac ttcgtcgttt cgctccagct gccgcactat
     1561 ttctggctgg ggctgcgcgg ctttgccggg gcgatcgcct ggctgctgtt gccgagcctg
     1621 atgctcatgg tcggcacatc gggccgcgga ccactgacga tcctgatcgg ctatcttggc
     1681 gcgctggggc tggcgtttgt cctgctttac ctgccgttcc tccagacgca cttcgcctgc
     1741 cagaaccgcc tggcggcgtt gttcgagtgg agcgccgtgc gggcccagtt ccgccgcgcg
     1801 ccgatcgctt attggctggc cctggcgatc acgctgctct tcgcgctgcc gctctatctg
     1861 ctcaagattg agccgccgcc gcgcgaactg tggtggaccg tgacgatctt cttcatcctg
     1921 ttcatttacc cggcccggct gctcaccggc tgggccgtcg gccgcgggct gcaccacgct
     1981 aagccgcggt tcttcctgtt ccgctggatg gcccgcctgg ccgcagtccc gatcgtgctg
     2041 ttttacgtgt tcatcctgtt cttcacgcag tacacgtcgt tcctcggccc ggcgagcatt
     2101 ctggagcagc acgcgttcct cgtgccggtg ccgttcctgg cgatgtagcc ggcgcgagcg
     2161 ctacttcgat ttgagagaca gcggcaggtc gttcgcgccc ggcttgacct ccacgtgcag
     2221 gtcgctcgtg gaaacggtcg cgtacttcgc cggcgtgatc cgctccggcg gaacttcggg
     2281 ggcgtaggga tccttcggcg gcggcagggg tttcgtggca acgacgacgg cgacatattt
     2341 cccgggcgag aggcccgcgg ccgtgcccgt gctgagcgtg tacttgccct ccgcgttgat
     2401 ctgaccatac gcggccgcgc ctgggcttcc atcgggatag aaactgacgt cgccggtcgt
     2461 cagcggctgg ccgtcgagcg tgaccgcgcc cgtaacggac gcagcattcc cgcccaggca
     2521 gcccgccacg agggagatcg ccgccagcac accgacgtag ccagcgaggc acagtcgccg
     2581 gcggccgttt cgtgacaagg acatggagac taattcgctg ggctaaggaa gctgcgcgtt
     2641 ctcgccgccg tttttcgaac tcagccggcg atagagcgtg tagtcgatcg tctgcgacac
     2701 gaagtggacc gatccgtcag caaggcaaaa gtgcgccccg ctgggatgca ggctgcggaa
     2761 cgagataacg ttccaccagg aacccggcgt cggcggtttg gggaaatagt tgagcggcgc
     2821 gtggcaactg gcgtagtcgc cgttggcgaa gtacgcggcg gagtgaatgt tctgatccgg
     2881 cacgtcctcg cccaccatga acgtgttcgc cgtgccgtcg gtgatcgtcg ccagccggtt
     2941 cggctcctgg tagctgttgc gataaaacag gccgttgttg ccgatcgtgt tgtgcgtgtc
     3001 cggctcggtc ccttgatgga tgctggtcgc gccgcccatc cgcgtatcgc cgatcacgcc
     3061 cttgtaatta gtgaccgcaa cctcgatccc gccccactgg gccgcattgg tgatgatccg
     3121 tttgctcttg ccgtccgacg ggcactcgat gaccttgatc cgcgtgcgga tggccgtgcg
     3181 gcaggcgggc atctgaattc cgcccgtggc gctgaacatg ccgttgaagc cgggctcgaa
     3241 ctgggtgaag agggcgggct gctcgagttg cggcaagatg ctaagaatcc agcccttgcc
     3301 gttcacctgg ggcgccggcc gtggattctc ccgcgcatac gaaatgctga tcggcagcgt
     3361 cagatggacg tcgtggtaat tgtgaatcgc gatgccgaac tgcttcaggt tgttgctgca
     3421 cgccatccgg cgggcagcct cgcgggcggc ctgaacggcc ggcagcagca gcgctaccag
     3481 gacgccgatg atggcgatga cgacgagcaa ttccaccaac gtaaacgcgc gacgcgcagc
     3541 aggttccgac atggagcacc tcgctttgcg gagcagaacc gaccccaaga attccgcgca
     3601 cagacgcctg cattgtgcga cgccggccag gtcgggtcaa caaaaacagt gcgattcttg
     3661 cggacttgcc gtctcaccgc ggcagcggta agggcgactt ttggggcggc cgctgggctt
     3721 cctgcggcgg gcgagcgccc ggcggcggat tgatgaccga aaccacgtcg gactgcactt
     3781 ccttcttcca gccgctggga acggtcggct cgtaaaactc gcggcggaag atatcgaggg
     3841 ccttcagcaa atcgcccacg gccatcttgt cggtcgtcgt ctgccggctg gccgcgggaa
     3901 agacgtaggt cttgcgatag ggcgtcacct cgctcgagtt gggctcgaac acggtcagca
     3961 tgtcgggcaa aaagtccttc tcttccagca cgatgtggac catcttgaag ttgcgggcgt
     4021 cgtcgcgcga cttgggaacg gcttccagcc agtatttgcc gttgcccgtc tgcggcaggc
     4081 ggcggaccca gtagcgggcc ttgatcgtct cggccttcgc accgaacagg aacggcagcg
     4141 gcccgtcggc gatcgccttg ccttgcatct ccaccggcag cggcaacacg atcacgcgcc
     4201 gcgtgcgggc ctcgaactga aacacctgct tgccgtcgca gacccaatgc tcgccgagcg
     4261 atttgtcctg gacgacgtat ttcggcttct cgggcggcgc gggcgcggca gcaggctgcg
     4321 ccgcgggatt cggcctcggc ggcggctcat agaacgtgag cttctcgacg cggaacaatc
     4381 ccttgtcggg ctgagcgtac ttgatcactc cctccgaata acgctgggcc acgtcgagcg
     4441 gcgcgtgggc gttgtattcc cagcactgaa agttgcagct gaacgttttg attttgttgc
     4501 tgtggttctc ccagtaggcc agcaccctgt tgatccagtc tgcctcttcc ggcgacatcg
     4561 tcagcgccca ctgcggcggc tgcggcgagg gcgcgatgtt cgccggcggc aggttgccgg
     4621 gcggcggtgc ttgccccgcc ggctgttggc ccgcgaccgg gcggatcgga ttgccgccca
     4681 ccggctgcgg tggcggattt tgcgccggtc ccgactgggg accataggcc tgtcgctgct
     4741 gcggctgctg cgcttggccc ggctgcgggc caaaggactg ggccgtcgca atcgcaccgc
     4801 ttccaatgag gcaaaccgca atgccaatcg acaatcgtcg ggtcatccgt gacctccgtt
     4861 tccgctccaa agccgcgacc a
//
LOCUS       DASRJB010000572        13624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3265237, whole genome shotgun sequence.
ACCESSION   DASRJB010000572 DASRJB010000000
VERSION     DASRJB010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265237"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFB80_14935"
     CDS             <1..365
                     /locus_tag="VFB80_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35121.1"
                     /translation="IFGALHWLSAEPVYYAFFAIQSLTICIAQMLHGQTPRKASIIAG
                     AVILPLFMLIAANFSEQSSNDGLFCLLIPLIPCGAVLGYLTGTCAAGIFLVMDKAEAY
                     FKGEPLDAALHAPPSPAP"
     gene            362..652
                     /locus_tag="VFB80_14940"
     CDS             362..652
                     /locus_tag="VFB80_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35122.1"
                     /translation="MSETPHSVNRLDPPPVFNCVVNVARPDAGGIVVARVVNLPGIEG
                     RGRSEREALGQVVPAFKAAVAALVAAGQPIPWIASPAAPAQGESQRLIAVHL"
     gene            complement(662..1249)
                     /locus_tag="VFB80_14945"
     CDS             complement(662..1249)
                     /locus_tag="VFB80_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35123.1"
                     /translation="MALSDIDRQLLQRCLSRQSRAWEDFVDRFLGLVVHVVNHTAQSR
                     SISISTHDLEDLASEVFVAILADDFGVLRRFRGQSSLATYLTVISRRVVVRELLKRRI
                     VPNPADTTEMRNVEDETAGHEERVANREQVERLLDELRGDDADIVRLYHIEGKSYHEI
                     SRQTGVPENSIGPALSRARAKMRRVGSSVGTAAGS"
     gene            1607..1885
                     /locus_tag="VFB80_14950"
     CDS             1607..1885
                     /locus_tag="VFB80_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZN35124.1"
                     /translation="MTHVVCQPCFGCKYTDCVVVCPVECFYEGEQILYIHPDECIDCE
                     ACVPECPVEAIFHQDNVPEDQKDFIALNAEMAPQCPVITEKKKPLAEE"
     gene            2149..2457
                     /locus_tag="VFB80_14955"
     CDS             2149..2457
                     /locus_tag="VFB80_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35125.1"
                     /translation="MIHHTCDRCKRVIDPEQELRYVVRLEIEVAMDPLYDEELHEDRD
                     HLSEIDEILERADASADELGDEPLQKRRYDLCPQCYRKFAASPLGREKQPASLGFSHN
                     "
     gene            complement(2470..3213)
                     /locus_tag="VFB80_14960"
     CDS             complement(2470..3213)
                     /locus_tag="VFB80_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN35126.1"
                     /translation="MDPNWYRTFFDDITVDFWRQAATPEWTTAEVDLAWRELRLAAGS
                     RVLDCPCGHGRHVLELARRGCQVIGIDYSEYSLQVARESAAEQKLAVDLRRGDMQELP
                     SLPTCDAAITLGNSLFYCEHAATGRFFQSVARALRKGGRWLLNTGTVAESIIAHLKPE
                     LNYELGEFKARFSNTYHVADSCLETLAEITRGARTESRTIWQCFVTVGELRRMLAAAG
                     LKTIGLYGDAAGAPYALGSHQLYLVAERE"
     gene            3400..3597
                     /locus_tag="VFB80_14965"
     CDS             3400..3597
                     /locus_tag="VFB80_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35127.1"
                     /translation="MTSDAPQTWFVMLRDEEAAGKVIVPLKPFLRFSRKMDKQIARLE
                     RQLRKRYPQLGRRGKFLRRPR"
     gene            complement(3600..5300)
                     /locus_tag="VFB80_14970"
     CDS             complement(3600..5300)
                     /locus_tag="VFB80_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN35128.1"
                     /translation="MFYLMANNGPQQGRRYELKGDKWVLGRHPDCQIVIEVGAVSRNH
                     CQVIRDGSLFFVEDLGSRNGTFINDEPEKMEGRRQLKPGDLVRVCEVTFTFRSDDVAH
                     NVPPTGPVNKMIDGAGLGAFLADDEGQPPSSTIMSKLDVSSSSRGNLHVSASAEVKLA
                     AMVEIIQSLGRALALDDVLPQVLKSLFKIFVQADRGFIVLQTPDNKLIPRWVRLRRED
                     STDTLRISRTIIRHVMESKQAILSADAASDERFEMSQSIADFRIRSMMCAPLMDNEGN
                     AFGALQIDTLDQRQRFTKEDLELLVSTASQAAVAIQNAQLHEQALKQRELERDMALAT
                     EVQHGFLPDRKPELPGYEFFDYYQPASQIGGDYFDYIPLTGGRVAIVVADVVGHGVAA
                     ALLMAKVSAETRFALYSEPTPAAAITRLNERMCQSNLQRFVTMILLVLDPAAHRALIV
                     NAGHMAPLHRKSTGKIEEPGDSNAGLPLGVTDALGYEQSEIDIAPGDCLVLFTDGLNE
                     SIDANGAFYTIDRLRDLLRKLGPKPQAAGQGIVDDVRKFLGKAAQNDDMCLVCFGRAG
                     "
     gene            5712..8015
                     /locus_tag="VFB80_14975"
     CDS             5712..8015
                     /locus_tag="VFB80_14975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN35129.1"
                     /translation="MPSETPIDAIQAGAPPLAESPRLEPERTADAQIGAVATLLTSLE
                     APPPPPPAPVILDTTELAVENELVQVRLGIAASLFAALRAKHAPTAHHSLRVALSCSA
                     WSALLGLTDLERDELEVAALLHDIGKIGIADSILLKPAALMPEEYLVVERHRQIGVDI
                     LRNCCATSSILKIVQHSGDWFDGSRDGATHRGEDLPLGARIVAIVDAFDAMTSDQVYR
                     RALAQERALAELFQFAGTQFDPRLVQEFCAYVNADPVKLQTVVARYWLKELARDESNA
                     LWQRTPTRPSTVAYREGMFYGQLLESMHDAVLFVDSALRILMWNRAAERLTGIAAASI
                     VHKHWSPTLLGMRDDKGQVLDDDVCPVIRAIHSGVQTLRRLSIAGRSGQRLEVDAHLV
                     PVHGKGGAAHGAALLLHDASSQITLEQRIQSLHEKATRDPLTQVANRAEFDRILASYV
                     ESHLERRIPCSLILCDLDFFKRVNDTYGHQAGDEVLITFAALLRRHCRSGDLVARYGG
                     EEFVLLCADCDNATATRRAEELRQEVAELPMAVLQGRSVSASFGVTEVQSGDTPETML
                     RRADRALYQAKSSGRNTVVQLGSGFGDQDAAPKRSSWFSWLLPTRPDQVLQRTLATGV
                     PFHVVVEKMRGFVADHHAQIDSVTENYLVLKVESGQELLRRAADRLVPFVIEMRFDES
                     SSPAQSGAPGKIVRTVIHVTIRPQRGRDRRRRDVLEKAQRLLASLKCYLIAQEHNPKS
                     KSATPLTPTGVEKDRLQAAAKSWTESA"
     gene            complement(8052..9329)
                     /locus_tag="VFB80_14980"
     CDS             complement(8052..9329)
                     /locus_tag="VFB80_14980"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN35130.1"
                     /translation="MKALLDLPLEVKLAALALIGLAVGSAVNWAIYALAWHSRPISPW
                     QRPDPKALPRRWADYLPLVGWLGLAREASLHGKGFWVRPLAIELFIAVGLPALYWWEI
                     TEHLAPPVMGVRAPQAMLHWEFLSHAILILFLTVATFIDFDEQMIPDTITVPGTLIGL
                     LLAGLAPGSLLPVFRIINVALGALGYGPLLLTSSQLWPGWLDGWQGLALGAGIFCCWC
                     LVLIPALATLRRGWQKAIQYYFASIIREQTWWQMLILAAIGSAYIAFIWRGGGPAWQS
                     LLTALVGLAGGGILVWGVRIVGYVALRKEAMGFGDVTLMAMIGVFLGWQTCLVVFFLS
                     PLAALIVSLTQWVLTGRRDIAFGPYLALTAVGVIVNWNAVWAFAGPLFAAGMLVPAMV
                     AAGLLLMMGLLMLWRIIEQALFAGEKEPGTKSR"
     gene            complement(9326..9856)
                     /locus_tag="VFB80_14985"
     CDS             complement(9326..9856)
                     /locus_tag="VFB80_14985"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZN35131.1"
                     /translation="MNLFLIGYRGSGKSTVAAALAERLRWPWIDADAELERRAGLSIK
                     EIFAREGESGFRQSEADVVQDLAGRDGWIVALGGGAVLRDDNRRAVAGRGKIVWLKAS
                     PETLAKRIHADPTTAERRPNLTAQGGLAEIRELVTRRAPLYAAWADLTVVAEEQSPAE
                     IADRICRELHLETTTP"
     gene            complement(9853..11361)
                     /gene="aroE"
                     /locus_tag="VFB80_14990"
     CDS             complement(9853..11361)
                     /gene="aroE"
                     /locus_tag="VFB80_14990"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN35132.1"
                     /translation="MAEASLLCVTIGRGRHRAMIAEHKHLAEQGCKLVELRLDFLVTE
                     PNLKRLLPERPCPVIVTCRREKDGGRWVQGETERMMLLRQAIAAGVEYVDLEEDIAAQ
                     IPRFGKTKRVISYHNFQETPEDLTHLHARLSELDADVVKIATMAHDPHDNLRVMRLMR
                     TSRVPTIAICMGEFGQPSRILAGKFGAPFSYATFSAERTMAPGQLSYEQMKEVYHYEK
                     INPDTEVYTVIADPVAHSLSPHIHNAAFEAAGLNKVYVPFRVRPEDLGQFLSDCRELA
                     VKGVSVTIPHKEEILKYKSKCDEQTDEIGASNTLVFKDEDRIAYNTDYRAAMNSIDTQ
                     FGGDETNSGLRTKSALVLGAGGVARAIVVGLRRRRAAITVTSRKLDRADDLARFCGGQ
                     SVPWELREGVNPDLVINCTPVGMHPNVDESPLPVSQLRRDMVVFDTVYNPEQTLLLKQ
                     AREAGCTVITGVDMFVGQAGLQYKLFTGEDPNVDLMRQVVRRTISAVRRQNG"
     gene            complement(11896..12369)
                     /locus_tag="VFB80_14995"
     CDS             complement(11896..12369)
                     /locus_tag="VFB80_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35133.1"
                     /translation="MFLEYWPWILAAAGVALAAAALGWWLLRRPPRRPRIERARKLFH
                     LQRERVEHRLFMLASKSGMPRGLEWVECDFEDDVSFARDRHTGRLRALVAVTIRFKAI
                     EGGPMEDNPNVDNLRAASAVFHLDGDDWGTDGRVMFNVNPTEAIQLYREELELVE"
     gene            12515..13174
                     /locus_tag="VFB80_15000"
     CDS             12515..13174
                     /locus_tag="VFB80_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35134.1"
                     /translation="MAIEILCPGCSRTLRVPDEHAGKQVRCPACQQISIAPGGTTSPV
                     AAKDGRWHLRTAGGPTYGPLDWRQVQEWAAEGRVTAGCQLADDADGTWRPATEFFPNL
                     RRPEVTAANTGPQTYPWTAEQLAEPGAATRQYVNPHRGGLILVLGLLGFMTCPIFSLM
                     AWVMGSHDLNEMRAGRMDRSGEGLTQAGQVLGMILSLLCIMGCVLMLFVFLLAAIAGN
                     F"
     gene            13181..>13624
                     /locus_tag="VFB80_15005"
     CDS             13181..>13624
                     /locus_tag="VFB80_15005"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HZN35135.1"
                     /translation="MPIETVCQTCGRQLRIADEHAGKTARCPACQTIYTVPQAASLSQ
                     PVHWPAAASASPGGIDRWHLRTPDGLTYGPVARAELDQWLQQGRITAQSQVLCEADGQ
                     WVFAAKLYPLLGATAPPASAPAPQATTNTYPYASQFADRPYTNMPY"
BASE COUNT         2360 a         4642 c         4308 g         2314 t
ORIGIN      
        1 ccattttcgg cgcgctgcac tggctcagcg cggagccggt ctattacgca ttcttcgcga
       61 ttcagtcgct gaccatctgc atcgcccaga tgctgcacgg ccagacgccg cggaaagcct
      121 ccatcattgc cggcgcggtg atcctgccgc tgttcatgtt gatcgccgcg aacttcagcg
      181 agcagagtag caacgacggg ctgttctgcc tgctcattcc gttgattccg tgcggggccg
      241 tgctgggata cctcacgggc acctgcgccg ccggcatttt cttggtgatg gacaaggccg
      301 aggcctactt caaaggcgag ccgttggatg ctgctttgca cgctccgccg agcccggccc
      361 catgagcgaa acaccgcaca gcgtaaatcg cctcgatccg ccgccggttt tcaactgcgt
      421 ggtaaatgtc gcgcggcccg acgccggcgg catcgtcgtc gcccgcgtcg tcaacctgcc
      481 cggcatcgaa ggccgcgggc gcagcgagcg cgaggcgctg ggccaggtcg ttccggcatt
      541 caaagcggcc gtcgccgcgc tagtcgccgc cggtcagccg attccctgga ttgccagccc
      601 cgcagcgccg gcgcagggcg agtcccagcg gctgattgcg gtacacctgt aagagagcca
      661 actaggaacc ggctgccgtg ccgacgctgc tgcccacgcg gcgcattttt gcccgggcgc
      721 ggctcagagc ggggccgatc gagttctcgg gaacgccggt ctgccggctg atctcgtggt
      781 acgatttgcc ctcgatgtgg tacagccgga cgatgtcggc atcgtcgccg cggagctcgt
      841 cgagcagccg ctcgacctgc tcgcggttgg cgacccgctc ctcgtgaccg gccgtttcgt
      901 cctcgacgtt ccgcatttcg gtggtgtcgg ccgggttggg aacgattcgc cgcttgagca
      961 actcgcggac cacaacccgc cgcgaaatca cggtcaggta ggtggccagg ctgctttgac
     1021 cgcggaaccg ccgcaggacg ccgaaatcgt cggccaggat ggccacaaag acttcgctgg
     1081 caagatcttc caggtcgtgg gtggaaatgg agatcgaccg cgactgggcg gtgtggttga
     1141 cgacgtggac gaccagtccc aggaaccgat cgacgaaatc ttcccacgcc cgcgattggc
     1201 gggagaggca gcgctgcaag agctgacgat cgatgtcgga aagcgccacg gcagtttcct
     1261 caaagttgcc cccacctttg gcaactcggc cggccgctag cagagtgcac ggccagcgcc
     1321 taaccatctg gatcgtctca ttttacgtcc ctcctccaac cgaaacaagc ttaacgtttt
     1381 gcccgggaat gcgggttgag cgggggattt ggggccgctt gacactcgcg ggggtcagga
     1441 ctacgattcc cgaaattcag gaaggaccgc tcaagtcgtt ccctgataac gacttgtcgc
     1501 ggctgacgca gattcgccca agcaggctgt ccgccactgt tggcactcat tcagcgcacg
     1561 cgccgctcca ttcttcgcac cgtatcgcac gaggagacat tgccccatga cccacgttgt
     1621 ttgccagccc tgcttcggct gcaagtacac ggactgcgtc gtcgtttgcc ccgtcgagtg
     1681 cttctatgaa ggggagcaga tcctttacat ccacccggat gaatgtatcg attgcgaagc
     1741 ttgcgtgccg gaatgccccg tggaggcgat cttccaccag gacaacgtgc ccgaagatca
     1801 gaaagacttc atcgccctca acgccgaaat ggcgccgcag tgtcctgtga ttaccgagaa
     1861 gaagaagccg ctggcggaag agtagcgggc cggcggtcgg cgcgtagcgg ctgaagaccc
     1921 gcgctggatt tcggcgcggg tttgtcgttt cttgccacgc gcgagagcac cgcgcgccgt
     1981 taaagtcaag ccctttatcg tccgcgaaat tggattattc gcgcgcggcg agaaaaccgc
     2041 ttcgctcgac ttgtggcgat tcgccgatcg gcggtaggat gagggggtcg cctttctgag
     2101 aggtcttttg cgaggcgact ttcatcatcc gcttcctggg gcccgtccat gatccatcac
     2161 acctgtgacc gctgtaagcg cgtcatcgac ccggagcagg agttgcgata cgtagtgcgc
     2221 ctggaaattg aagtggcgat ggatccgctc tacgacgagg agctgcacga agaccgcgac
     2281 catttgtcgg agatcgacga aatcctcgag agggccgatg cctcggccga cgagttgggc
     2341 gacgagccgc tccagaagcg gcgttacgat ctgtgcccgc aatgctatcg caagttcgcc
     2401 gcgagcccgc tgggccgcga gaagcagccg gcctcgctgg gtttcagcca caactaggca
     2461 gaaagctggc tattcccgtt cggcgaccag atacagctgg tggctgccca aggcgtaggg
     2521 tgcgcccgcc gcgtcgccat agaggccaat ggtcttcagc ccggcggcgg ccagcatgcg
     2581 ccgcaattcg ccgaccgtta caaagcattg ccagatcgtg cggctctcgg tgcgggcgcc
     2641 gcgggtgatc tccgcaagcg tttccaggca actgtcggcc acgtggtagg tgttgctgaa
     2701 ccgggccttg aactcgccca gctcgtagtt cagctccggc tttaagtgag cgatgatcga
     2761 ttcggcaacc gtgccggtgt tcagcagcca gcgaccgccc ttgcgcaggg cgcgagcgac
     2821 cgactgaaag aaccggccgg tggcggcgtg ctcgcagtag aacagtgaat tgccgagcgt
     2881 aatcgccgca tcgcaagtcg gcaggctggg cagctcttgc atgtcgcccc gccgcagatc
     2941 gaccgcgagc ttctgctctg ccgcggactc gcgggcgacc tgcagggaat attccgagta
     3001 gtcgatcccg attacctgac agccgcgtct ggcgagctcc aggacatgcc ggccgtggcc
     3061 gcaggggcaa tcgagaacgc ggctgccggc ggccagccgc aactcgcgcc aggccagatc
     3121 gacttccgcc gtagtccact cgggcgtcgc cgcctgccgc cagaaatcga ccgtaatgtc
     3181 gtcaaagaag gtgcgatacc agttgggatc catgcggtgc tccgggatgg ccccaggatg
     3241 gccagcgccc gcgccgggtt cgcgcgattc cggaaaatcc ggcgacccgc gctgtgcagc
     3301 gcgaacttat cggggggctg cggcgtcaca agatgagagg tcacggtgcg aataccagat
     3361 agggacacgg ggccgccaca gcgaaggggt gaaactgaag tgaccagcga tgcgccgcaa
     3421 acctggtttg tgatgttgcg ggacgaagag gcagcgggca aagtcatcgt ccctctcaag
     3481 ccgttcctgc ggttcagccg gaagatggac aagcaaatcg cccgactcga gcgccagctc
     3541 agaaaacgct atccgcagtt aggccgccgg gggaagttcc ttcgccgccc caggtagccc
     3601 taaccggccc ggccgaagca aaccaggcac atgtcgtcgt tctgggccgc cttgcccagg
     3661 aacttccgca cgtcatccac aatcccctgg cccgccgctt gcggcttggg cccgagcttt
     3721 cgcagcagat cacggagccg gtcgatcgtg taaaacgccc cgttggcgtc gatcgattcg
     3781 ttgaggccgt ccgtgaacag gaccaggcaa tcacccggag cgatgtcgat ctcactttgc
     3841 tcgtagccga gggcatcggt cacgcccaga ggcaagccgg cgttcgagtc gccgggctct
     3901 tcaatcttgc cggtggactt gcggtgtagc ggcgccatgt ggccggcatt gacgatgagc
     3961 gcccggtggg cagctggatc gagcaccagc aggatcatcg tcacgaaccg ctggaggttc
     4021 gactggcaca tccgctcgtt cagccgggtg atcgcagcgg cgggcgtcgg ctcgctgtag
     4081 agggcgaacc gcgtttcggc cgagactttg gccatgagca gcgccgcggc gacgccgtgc
     4141 ccgaccacgt cggccaccac gatggccacc cggccgccgg tgagcgggat gtagtcgaaa
     4201 tagtcgccgc cgatctgcga ggcgggctgg tagtagtcga aaaactcgta gcccggcagc
     4261 tccggcttgc gatcaggcag aaagccgtgc tggacctcgg tggccagcgc catgtcgcgc
     4321 tccagctcgc gctgcttgag ggcctgctcg tgcagctgcg cgttttgaat cgcgaccgcg
     4381 gcctgtgagg ccgtgctgac caatagctcc aggtcttcct tcgtgaaccg ctgccgctgg
     4441 tcgagcgtgt cgatctgcag cgccccgaag gcgttcccct cgttgtccat cagcggggcg
     4501 cacatcatcg agcggatgcg gaaatcggcg atgctctggc tcatctcgaa ccgctcgtcg
     4561 ctcgccgcgt cggctgaaag gatcgcctgc ttcgattcca tgacgtggcg gatgatcgtg
     4621 cggctgatcc gcagcgtatc cgtcgaatcc tcccggcgga ggcggaccca gcgggggatc
     4681 agcttgttgt cgggggtctg caacacgatg aagccgcggt cggcctgcac gaaaatctta
     4741 aacaggctct tgagtacctg cggcaacaca tcgtctagcg ccagcgcccg tcccaagctt
     4801 tggataatct ccaccatcgc cgccagcttg acttcggcgc tggcgctgac gtgcaggttg
     4861 ccgcggctgg agctcgacac gtccagcttg gacatgatcg tcgagctggg cggctggcct
     4921 tcgtcgtcgg ccaggaacgc tcccaggccc gccccatcga tcatcttgtt caccggcccg
     4981 gtcggcggca cgttgtgtgc aacgtcatcc gaccggaacg taaacgtcac ctcgcagacc
     5041 cgcaccaggt cgcccggctt gagctgccgc cggccttcca tcttctccgg ctcgtcgttg
     5101 atgaacgtgc cgttgcggct gcccagatcc tcgacgaaaa acaggctgcc gtcgcggatt
     5161 acctggcaat ggttgcggct gacggccccg acctcaatca cgatctggca gtccgggtgc
     5221 cggccgagaa cccacttgtc ccccttcagc tcgtagcgcc ggccctgctg cgggccgttg
     5281 tttgccatca gataaaacat gaccgcggaa tcctgcgaac cacgggcgcc aggcgtgaac
     5341 gaatcctgaa tcggcgaatt cgctggggag atgccgccgc attcacgggt tgattgtaga
     5401 gagcgaccgg aatgagcgtc aaccgaacgg aatggcaaca gttcctgccc gacttttttc
     5461 ggcaaattcg gatcgccgct cagtagcgct tcctcttgcg cggatggcag ccccggcatt
     5521 ctcgccacaa tcgacgcgct gggtgccaaa ccccttctct ctgctctcgc cccgcgccgc
     5581 caggtttgcc gcaagtcccc ccacgaccta actttgcgca ggaaggcggc cgattcgcgg
     5641 ctgccggaga cgatcgttcc ccctaatcgg tctgcgccga agcggactct cggcccggat
     5701 cgttgactgg catgccctct gagactccga tcgacgcgat ccaagcgggc gcgccgccgc
     5761 tcgccgaatc tccgcgcctg gagccagagc gaaccgccga cgcgcagatc ggcgccgtcg
     5821 ccacgctgct gacctcgctc gaagccccgc ctcctccgcc gcctgctccc gtaatcctcg
     5881 acacgaccga gctcgccgtc gaaaacgagc tggtgcaggt gcggctggga atcgccgcca
     5941 gcttgttcgc cgcgcttcgc gccaagcacg ccccgaccgc gcaccacagc ctccgcgtgg
     6001 ccctcagctg ttcggcctgg agcgcgctgc tgggcctgac cgacctcgag cgggacgagc
     6061 tggaagtcgc cgcactgctc cacgacatcg gtaagatcgg catcgccgac tcgatcctgc
     6121 tcaagccagc cgcgctcatg ccggaggagt acctcgtcgt cgagcgccac cgccagatcg
     6181 gcgtcgatat cctgcggaac tgctgcgcca cgtcgagcat cctcaagatc gtgcagcact
     6241 ccggcgactg gttcgacggg agccgcgacg gcgcgaccca ccgcggcgaa gacctgcccc
     6301 tgggcgcgcg gatcgtggcg atcgtcgatg ccttcgacgc gatgaccagc gaccaagtct
     6361 atcgccgggc gctggcccag gaacgggcgc tggcggagct gttccagttc gccggcacgc
     6421 aattcgaccc gcggctggtg caggaattct gcgcgtacgt gaacgccgac ccggtcaagc
     6481 tgcaaacggt cgtcgcgcgg tattggctga aagaactggc ccgcgacgaa agcaacgccc
     6541 tctggcagcg cacgcccaca cgaccttcga ccgtcgccta tcgcgaaggc atgttctacg
     6601 gccagctgct ggagagcatg cacgacgccg tcctcttcgt ggacagcgcc ttgcggattc
     6661 tgatgtggaa ccgcgccgcc gagcggctga cgggcatcgc cgccgccagc atcgtccaca
     6721 agcactggtc gcccacgctg ctcggcatgc gcgacgacaa gggccaagtc ctggacgacg
     6781 acgtctgccc cgtcattcgg gccattcaca gcggcgtgca gacgctgcgg cggctgtcga
     6841 tcgccgggcg cagcggccag cggctggaag tggatgccca cctggtgccg gtgcacggca
     6901 aagggggcgc ggcccacggc gcggccctgc tgctgcacga cgcttcgagc cagatcacgc
     6961 tcgagcagcg gattcagtcg ctgcacgaaa aggccacccg cgatccgctg acccaggtcg
     7021 ccaaccgggc cgagttcgac cgcatcctgg cgagctacgt cgagtcgcac ctggagcggc
     7081 ggattccctg cagcctgatc ctttgcgacc tcgacttctt caaacgcgtc aacgacacct
     7141 acggccacca ggccggcgac gaagtgctga tcacgttcgc cgcactgttg cggcggcact
     7201 gccgctcggg cgacctcgtg gcccgctacg gcggcgagga gttcgtgctg ctctgcgccg
     7261 actgcgacaa cgccacggcc acgcggcggg ccgaagagct gcggcaggaa gtggccgagc
     7321 tgcccatggc ggtcttgcag ggccgctcgg tctcggccag cttcggcgtc accgaagtgc
     7381 agtccggcga tacgcccgag acgatgctgc ggcgggccga ccgggccttg taccaggcca
     7441 aatcgagcgg ccgcaacacc gtcgtccagc tgggctccgg attcggcgac caggacgctg
     7501 cccccaagcg ctccagctgg ttctcgtggc tcttgccgac tcgccccgac caggtcctgc
     7561 agcggacgct ggccaccggc gtgccgttcc acgtcgtcgt cgagaagatg cgcggcttcg
     7621 tcgccgacca ccacgcccag atcgattcgg tcaccgagaa ctacctcgtg ctcaaggtgg
     7681 aaagcggcca ggaattgctc cgccgggccg ccgaccggct ggtgccgttc gtgatcgaga
     7741 tgcggttcga cgagtcgtcg tcgcccgcgc agtccggcgc gccgggcaag atcgtccgga
     7801 ccgtgatcca cgtcaccatc cgcccgcagc gcggccgcga tcgccgccgc cgcgacgtgc
     7861 tggaaaaagc ccagcggctg ctggccagtc tcaagtgcta tctcatcgct caggaacata
     7921 atcccaagtc caaatcggcc actcctctca cgcccaccgg cgtcgaaaag gaccgcttgc
     7981 aagccgccgc caagagctgg acggaatcgg cgtgactcag gggccaggag ccattcatca
     8041 ctgtcaactg gttaccggct cttggtccct ggctccttct cgccagcaaa cagcgcctgt
     8101 tcgatgatcc gccagagcat cagcaggccc atcatcagca agaggcccgc ggcgaccatg
     8161 gccggcacga gcattcccgc cgcgaatagc ggccccgcga acgcccacac ggcgttccaa
     8221 ttcacaatca cccccaccgc ggtcagcgcc aggtaggggc cgaacgcgat gtcgcgccgc
     8281 cccgtcagga cccactgggt caaggagacg atcagcgccg ccagcggcga caggaagaac
     8341 accaccaggc aggtttgcca gcccagaaac actccgatca tcgccatcag cgtcacgtcg
     8401 ccgaagccca tcgcctcttt ccgcaaggcg acatagccca cgatccgcac gccccacacc
     8461 aaaatgccgc cgccggccag gccgaccagc gccgtcagca ggctctgcca tgccgggccg
     8521 ccgccgcgcc agatgaacgc gatgtacgcg ctgccgattg ccgccaggat cagcatctgc
     8581 caccaggtct gctcgcggat gatgctggcg aagtagtact ggatcgcttt ctgccagccg
     8641 cggcggagcg tggctagcgc gggaatcagc accaggcacc agcagcagaa gattcccgcg
     8701 cccagcgcca gcccctgcca gccgtcgagc cagccaggcc agagctgcga cgacgtgagc
     8761 agcagaggtc cgtaaccaag cgcccctaag gctacgttga tgatccggaa caccgggagc
     8821 agcgaaccgg gcgcgaggcc tgccagcaag aggccgatca gcgtgccggg aacggtgatc
     8881 gtgtccggga tcatttgttc gtcgaagtcg atgaacgtcg ccacggtcag gaacaagatc
     8941 aggatcgcat ggctcaggaa ctcccagtgc aacatcgcct gcggcgctcg cacgcccatc
     9001 accggcggcg ccaagtgctc ggtaatttcc caccagtaca gcgccggcaa tccgaccgcg
     9061 ataaacagct cgatcgccag cgggcgaacc cagaatccct tgccatgcag cgatgcctcg
     9121 cgcgccaggc ccaaccagcc gactagcggc aaatagtcgg cccaccgccg cggcaacgct
     9181 ttggggtcgg gcctctgcca ggggctgatc ggccgcgagt gccacgccag ggcgtaaatc
     9241 gcccagttca cggcgctgcc caccgccaat ccgatcagcg caagcgccgc cagcttgact
     9301 tcgagcggca ggtccagaag cgctttcacg gggtggttgt ttccaggtgc agctcgcggc
     9361 aaatgcggtc agcgatctcg gcgggcgatt gctcctccgc cacgaccgtc aggtccgccc
     9421 acgcggcata gagcggcgcg cggcgagtta caagctcgcg aatttcggcc agaccgccct
     9481 gggctgtcaa gttcggtcgc cgctcggccg tcgtcgggtc ggcatgaatg cgcttcgcca
     9541 gcgtttcggg cgaggccttc agccagacga ttttgccgcg gccggcgacg gcccgccggt
     9601 tgtcgtcgcg caggacggcg ccgcctccca aggcgacgat ccacccatcg cggccggcga
     9661 ggtcctggac cacgtcggcc tcgctctggc gaaatccgct ctcgccttcg cgggcgaaaa
     9721 tctccttgat cgacaggccc gcccgtcgct ccagctctgc gtcggcgtcg atccacggcc
     9781 agcggagccg ctcggccagc gcggcggcga ccgtcgattt gccgctgccc cgatagccga
     9841 tgaggaacaa gttcatccgt tttgacgccg caccgcgctg attgtccgcc ggacgacctg
     9901 ccgcatcagg tccacgttcg gatcttcgcc ggtgaacagc ttgtattgca gccccgcctg
     9961 gccgacgaac atgtccacgc ccgtgatcac cgtgcagccg gcctcgcggg cctgcttgag
    10021 cagcagcgtc tgctcggggt tatagaccgt gtcgaacacg accatgtcgc gccgcagctg
    10081 cgagacaggc agcggcgact cgtccacgtt ggggtgcatt cccaccggcg tacagttgat
    10141 cacgaggtcg gggttcacgc cctcgcgcag ctcccacggc accgactgtc cgccgcagaa
    10201 ccgggccagg tcgtcggccc gatcgagctt gcgcgaagtg acggtgatgg cggcccgccg
    10261 ccgccgcagg ccgaccacga tcgcccgcgc cacgccgcca gcaccgagca ccagggccga
    10321 cttggtccgc agccccgagt tcgtctcgtc gccgccgaac tgggtgtcga tgctgttcat
    10381 cgccgcccga tagtcggtgt tgtaggcgat ccggtcttca tccttgaaga ccagcgtgtt
    10441 ggaggcccca atctcgtcgg tctgttcgtc gcacttcgac ttgtatttca ggatttcttc
    10501 cttgtgcggg atcgtgacgc tcacgccctt caccgccagc tcgcggcaat cgctgaggaa
    10561 ctgccccagg tcctccggcc gcacgcggaa cggcacgtag accttgttca ggcccgccgc
    10621 ctcgaacgcc gcgttgtgaa tgtgcgggct caggctatgg gcgaccggat cggcgatcac
    10681 cgtatagacc tcggtgtcgg ggttgatctt ctcgtagtga tagacctcct tcatctgctc
    10741 gtacgacagc tggccgggag ccatcgtccg ctcggccgag aaggtcgcat agctgaacgg
    10801 cgctccgaat ttccccgcca ggattcgcga gggctgcccg aactcgccca tgcagatcgc
    10861 gatcgtcggc acgcggctgg tccgcatcag ccgcatgacc cgcaggttgt cgtgcgggtc
    10921 gtgggccatc gtggcgatct tcaccacgtc ggcgtcgagc tccgacagcc gggcatgcag
    10981 gtgcgtcagg tcctcgggcg tttcctggaa attgtggtag ctgatgaccc gcttggtctt
    11041 gccgaaccgc gggatttgcg ccgcgatgtc ctcctccaga tcgacatact cgacgcccgc
    11101 cgcgatcgcc tgccgcagca gcatcatccg ctcggtttcg ccctgcaccc agcggccgcc
    11161 gtccttctcg cggcggcagg tgacgatcac cgggcaaggc cgctccggca gcagccgctt
    11221 caggttcggc tccgtgacca ggaaatccag ccgcaactcc acgagcttgc agccttgctc
    11281 ggccaggtgc ttgtgctcgg cgatcatcgc ccggtgccgc ccgcggccga tggtcacgca
    11341 caacaaggaa gcttcagcca tgggagggtc cgcaaggggc gggaacgaag cgccactatc
    11401 gtaggatggt tagcctcacc ggtcgaatca aggcggcgga atcgaccgga gccgccgcca
    11461 gatcagtgcc tatgcgcgca tattgcgaag aaagtgttgg ggggcggaac tggtcgcaag
    11521 ttattgtggc caaacgagtt gcattgcaat tcaggcgatt ggcattatgc gcactatgcg
    11581 cgcgggcttg gcgtgcacga ccgcgaatcg catcgcggat gcaccggggg gaactcagtg
    11641 ctcgccctgg tctgcatatg catatttcaa aaatttccgg cagcagagtt ttcggcgcaa
    11701 ctctgtgttg cgataagact taacgtcgat tccaaggcac gcgccgtatg cagtttatgc
    11761 acgttaggct gagatgcaac gatcccgtcc ccagccgcaa gcattgtaac aatcggtaca
    11821 ctaatgtcaa tgaagtaccg agtcccagcc acgccgtacc ggctctgccg ctggagggcc
    11881 tcgactcgcg aggcactact ccacgagctc cagctcctcg cggtagagct ggatcgcttc
    11941 ggtggggttg acgttgaaca tcacccggcc gtcggtgccc cagtcgtcgc cgtcgaggtg
    12001 gaagacggcg ctggcggccc gcaggttatc gacgttgggg ttgtcttcca tcgggccgcc
    12061 ttcgatggcc ttgaagcgga tcgtcacggc caccagggcc cgcagacgcc cggtgtgccg
    12121 gtcgcgggcg aaagagacgt cgtcctcaaa atcgcactcc acccattcca ggccgcgggg
    12181 catcccgctc ttcgacgcca gcatgaatag ccggtgctcg acgcgttccc gctggaggtg
    12241 gaacagcttg cgggcccgct cgatgcgggg cctccgcggc ggccgccgca gcagccacca
    12301 gccgagggcc gcggcggcca gcgccacgcc cgctgctgcc agaatccacg gccagtattc
    12361 aaggaacatg actgatccgc cgtcaaacag gtcgcggagc accctttacc ccgatattgt
    12421 aggcgcgccg acgaatagct ggcaaccgat ttgccccgct gcgcctcgcg atgctccgcc
    12481 cgccgcttag ccccggcctg ctggccggct ccccatggcc atcgaaattc tctgccccgg
    12541 atgcagccgc acgctgcgtg tgcccgacga gcacgccgga aagcaggtcc gctgccccgc
    12601 ttgccagcaa atcagcatcg ccccgggcgg cacgaccagc ccggtcgctg cgaaggacgg
    12661 ccgctggcat ctgcggacgg ctggcggccc cacgtacggc ccgctcgact ggcggcaggt
    12721 gcaggagtgg gccgccgagg gccgggtcac cgccggctgt cagctggccg acgacgccga
    12781 cggcacctgg cggcccgcga ccgagttctt tcccaacctc cgccgtcccg aggtcacggc
    12841 cgcaaatacc gggccgcaga cctatccctg gaccgccgag cagctcgccg agccaggcgc
    12901 ggcgacgcgg caatacgtca atccgcatcg cggcggcctg attctcgtgc tgggcctgct
    12961 cggcttcatg acctgcccga tcttcagctt gatggcctgg gtcatgggca gccacgacct
    13021 gaatgagatg cgggccggcc gcatggaccg cagcggcgag gggctgacgc aggccgggca
    13081 ggtcctgggt atgattctgt cgctgctctg catcatgggc tgcgtcctga tgttgttcgt
    13141 ctttctcctg gcggccatcg cgggcaattt ttagtcgagc atgccgatcg aaaccgtttg
    13201 ccagacctgc ggtcggcaac tgcgcatcgc cgacgagcac gcgggaaaga ccgcgcgctg
    13261 cccggcctgc cagacgatct ataccgtgcc gcaggccgcg agtctttcac agcccgtcca
    13321 ctggcccgcg gctgccagcg cttcgccagg cgggatagac cgctggcact tgcgaacgcc
    13381 cgacggcctg acgtatggcc cggtggcgag ggccgagctg gaccagtggc tgcagcaggg
    13441 ccgcatcacg gcccagtcgc aagtcctgtg cgaggcggac ggccaatggg tttttgccgc
    13501 gaagctctat ccgctgctcg gggccacggc cccaccggca tccgcgccgg cgccgcaggc
    13561 gacaaccaac acttatccgt acgcatcgca attcgcggac aggccttaca cgaacatgcc
    13621 ctac
//
LOCUS       DASRJB010000573         7247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2703269, whole genome shotgun sequence.
ACCESSION   DASRJB010000573 DASRJB010000000
VERSION     DASRJB010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703269"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFB80_15010"
     CDS             complement(<1..415)
                     /locus_tag="VFB80_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN35136.1"
                     /translation="MPANLTPQYKKAEEEYRRAQTPEEELRCLELMLREIPKHKGTDK
                     LQAELKAKISKARHETEQAKKTAKKGHHGPHIQRQGAGRATILGGPNAGKSQLLARLT
                     RATPEVAPYPFTTREPQPGMMPWEDVQVQLVDTPPI"
     gene            complement(423..1589)
                     /gene="bioF"
                     /locus_tag="VFB80_15015"
     CDS             complement(423..1589)
                     /gene="bioF"
                     /locus_tag="VFB80_15015"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZN35137.1"
                     /translation="MSDPLGWLDDALADLDRRALRRTLTARRGPQRGDRIEIGGQSLV
                     NFGSNDYLGLASDPRVAAAVRAALGDYGWGSGASPLVTGRSDLHAELERKLAEFQGTE
                     AALLFPSGYAANVGAITALASKGDAIYSDELNHASLIDGCRLSGAAVHVYPHRDVGQL
                     RQLLIDSRQVRRKLIVTDSLFSMGGDRAPLEGLAELAQRYDAMLLVDEAHATAIFGAT
                     GRGLAEEAGVEDGVHIRVGTLSKALGSLGGFVVGGRTLIDWLANRARPYVYSTAAPAA
                     LAAAGLAALELIAGEPERRQTLQQLASFARHEFLRQRVNYSGAFSQIMPVRIGNPHQA
                     IAAAAHLRDSGFLVPAIRPPSVPEGQSLLRISLTCLHTERQVEDLVAALCEVAR"
     gene            1690..2559
                     /locus_tag="VFB80_15020"
     CDS             1690..2559
                     /locus_tag="VFB80_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35138.1"
                     /translation="MLPTKWLTRLEERTLPHFNQLVIWLLLATAMVLAVWRFEIRTGE
                     RSGFVSLLSWLPDSVVASPATWLACRGLLIAGIVLWALNRWLPWSCWLTVAGFTCVWS
                     LHVETTYNTAHIFHMANMLLVIQALWITGDARLIRERIAAGTFWQTPLVPRWVSLASI
                     AYIGIFHTAAGLSKLLYSGPQWATGTSLQLWTYLWGRPWSPTTQFLLSSRSFTRGLQV
                     LTLVVESSGILAIFPRLRTWIGLALLAFYAGVLATFDYGFQFNAMLTAIYFLPLESWI
                     TRWARKHQPSRAT"
     gene            2713..4911
                     /gene="glgP"
                     /locus_tag="VFB80_15025"
     CDS             2713..4911
                     /gene="glgP"
                     /locus_tag="VFB80_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZN35139.1"
                     /translation="MTTIDTSIVPEPNPFLAGELSAQSLYDKCWSLARNLYWTWHPEI
                     ISLFRDLDPIRWRQLDHNPIALLREFTAERLAQRAAEMVLYSRINYAHRRLKEYLANK
                     QTWAAWNAGVLGGKPVAYFSAEFGLHESIPIYSGGLGVLSGDHIKSASGLGVNLVAIG
                     LFYDQGYFKQQLDDTGWQREEYIDTKVENVPMEPALSPQGKPITVRVDTRNGPLFAKV
                     WMMHVGRVRLFLLDCDVEGNSPHDRELTSRLYGGDERVRIRQELVLGVGGVKALRALG
                     ITPGVYHLNEGHSAFAPLEVIRERMHDDGMRFDDAFREVARQTVFTTHTPVPAGHDRF
                     HGGLVEEHLGPLRDQLGISYEQLMGLGRVEPQNDQEAFTMTVIGLKLSRRANAVSQLH
                     GHISRRMWASLWPWRVEEEIPIGHITNGVHIPTWLAQQMLQLYDRHFPAQWIFRMGEP
                     EVWQNIHSVDPGELWETHNALKNLLLAFVRRRVSRQCRRRGESDDAVEAARNVLDPNV
                     LTIGFGRRFATYKRATMVINNLDLLNAICNDKERPVQFVFAGKAHPKDEPGKRLIQTI
                     ANLRKDPRFASRVAYVEDYDMNVCRHMIQGVDVWLNNPRRPLEASGTSGEKTVLNGGL
                     NLSILDGWWAEAYDGSNGFAIGQGHSHVDDAITDKRDAESLYRVIEDQVVPMYYDRDV
                     DGLPRHWVKRMMNSISTLAWRFSAHRMVMDYTRLCYVPAAGGLSSEMTTH"
     gene            5025..5942
                     /locus_tag="VFB80_15030"
     CDS             5025..5942
                     /locus_tag="VFB80_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZN35140.1"
                     /translation="MGHAHHHDHDHSGTASTTPAFAIGVALNAAFVVVEAAFGFWAGS
                     LALLADAGHNLSDVLGLLLAWGASYLACQRPSRRRTYGLRRSSIMAALANALLLLAAV
                     GAIVWEAIGRLIQPTPAAPVPVIVVAGIGVAVNTLTALLFLRGRHHDLNVRGAFLHMA
                     ADAAVSLGVVVAGIVIAFTGWQRVDPLLSLLIAGVIAWSTWDLLWQAVNLSLDAVPRG
                     IDPAAVRAYLAALPGVSEVHDLHIWGLSTTESALTVHLIKPGAALDDDWLAAVSHELH
                     DRFGIGHATIQVESGAGSQSCRLAPEDVV"
     gene            complement(5939..6808)
                     /locus_tag="VFB80_15035"
     CDS             complement(5939..6808)
                     /locus_tag="VFB80_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35141.1"
                     /translation="MPGMMEGLSTVNSFLRTMIALVVIGGVGVAGWFGYTTYNAKEID
                     ARKKAEQLTAATAALADAKTRLETAEADIAAKAEQIRERDAQITKLNEDVEKLETALS
                     LLKVDHRVARLTAVDQTKDEATGEVTTKVEFVELNDEGHPIDTPRLFPIRGDTVFLDN
                     WVVKFDDKYVESADLERGTSLVLFRRIFGEKQQPIDGFPLDDVGSAPRAYQRGGKMSD
                     FEKQIWGDFWAIANDAQRAEQLGIRAAHGLALSMKVQKGKSYTVQLRASDGLSFVPDA
                     AAPASGEVKPNER"
     gene            complement(6867..>7247)
                     /locus_tag="VFB80_15040"
     CDS             complement(6867..>7247)
                     /locus_tag="VFB80_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HZN35142.1"
                     /translation="AVLLIAECLDDCNLRKLHNEAIELGMTPLVEFYEPRNLERVLAA
                     GAALVGVNNRDLRTFAVDLGHTVRMRSQVPLDCVLVGESGIESRADVLRLQEAGVEAM
                     LVGESLMREPDIGRAVDLLLGRAA"
BASE COUNT         1159 a         2479 c         2327 g         1282 t
ORIGIN      
        1 tgatcggcgg cgtatcgaca agctgcacct gcacatcctc ccagggcatc atccccggtt
       61 gtggctcgcg ggtcgtgaac ggatagggcg cgacctcggg cgtggcccgg gtcaggcggg
      121 cgagcagctg gctcttgccc gcgttggggc cgccgaggat cgtcgcccgc cccgcgccct
      181 gccgttggat gtgcggcccg tgatgcccct ttttcgccgt cttcttcgcc tgttccgtct
      241 cgtgcctggc cttgctgatc ttggccttca gctcggcctg cagcttgtcg gtgcccttgt
      301 gcttgggaat ctcccgcagc atcagctcca ggcatcgcag ctcctcctcc ggcgtctgcg
      361 cgcggcggta ctcctcttcg gctttcttgt actggggagt gaggttcgcc ggcatggctc
      421 gattatcgcg ccacttcgca cagcgcggca acgaggtcct ccacctgccg ctcggtatgc
      481 aggcacgtca ggctgatccg cagcagcgac tggccctcgg ggacgctcgg cgggcggatc
      541 gccggtacca ggaagcccga gtctcgcaga tgcgcggccg cggcgattgc ctggtgcgga
      601 ttcccgatgc gaacgggcat gatctggctg aaggcgccgc tgtagttcac gcgctgccgc
      661 aagaactcgt gtcgggcgaa tgaggccaac tgctgcagcg tttggcggcg ctctggttcg
      721 ccggcaatca gctccagcgc tgccagcccc gcggcagcca gcgccgccgg cgcagcggtc
      781 gaatagacgt aggggcgggc gcgattggcc agccagtcga tcaaggtccg tccgcccact
      841 acaaaaccgc ccagactgcc gagcgccttg ctcagcgtgc caacgcggat gtggactccg
      901 tcctcgactc ccgcttcttc ggcaagcccc cgaccagtcg ccccaaaaat ggcggtcgca
      961 tgtgcttcat cgaccagcag catcgcgtca tagcgctggg ccagttccgc cagtccctcc
     1021 agcggcgcgc gatctccgcc catcgagaac agcgaatccg taacgatcag cttccgccgc
     1081 acttgacgcg agtcaatcaa aagctgccgc agttggccca cgtcgcggtg cggatacacg
     1141 tgcaccgccg ccccgctcag ccggcagccg tcgatgaggc tggcgtggtt gagttcgtcg
     1201 ctgtagatcg cgtccccttt gcttgcgagg gccgtgatcg cgccgacgtt ggcggcgtag
     1261 cccgagggga agagcagcgc agcctcggtg ccctggaatt cggccagctt gcgttcgagc
     1321 tcggcatgca ggtccgagcg gccggtgacc agcgggctgg cgccgctgcc ccagccatag
     1381 tcgccgagcg cggcccggac agccgcggcg actcgcggat cgctggcgag gccgaggtag
     1441 tcgttggagc cgaagttgac gagcgactgg ccgccgattt cgatccggtc gccccgctgc
     1501 gggccgcggc gcgcggtcag cgtacgccgc agcgcgcggc ggtccaagtc ggcgagcgcg
     1561 tcgtcaagcc aacccagggg atcggacatg acttgatcgt agtggcaacc tgccggcttg
     1621 ccaggggcgg cggatggcgt atgttcggcg gcacgccggc ctgggggccg gggctaagcg
     1681 agaccacctg tgctaccgac caaatggctg acgcggctgg aagagcggac gctgccgcac
     1741 ttcaatcagc tcgtgatctg gctgctcttg gccacggcaa tggtcctggc tgtctggcgg
     1801 ttcgagatcc gcaccggcga gcgatccggc ttcgtctcac tgctctcgtg gctgccggac
     1861 tctgtagttg ccagccccgc gacgtggctg gcctgccgcg ggctgctgat tgcgggcatc
     1921 gtgctgtggg ccctcaaccg ttggctcccc tggtcgtgct ggctgacggt ggccggcttc
     1981 acttgcgtct ggtcgctgca cgtcgagacg acctacaaca cggcgcacat ctttcacatg
     2041 gcgaacatgc tgctggtgat tcaagccctt tggattaccg gcgatgcgcg actgatccgc
     2101 gagagaatcg ccgccggcac gttttggcaa acgccgctgg tcccgcgctg ggtctcgctg
     2161 gcctcgatcg cttacatcgg catcttccac accgccgcag gtctctcgaa gctgctctac
     2221 agcggcccgc agtgggccac cggcacttcg ctgcaattgt ggacgtacct ttggggccga
     2281 ccctggtcgc cgacgaccca gttcctgctg agcagccgct cgttcacgcg cgggctgcaa
     2341 gtgctgacgc tcgtcgtcga atcgtccggc attctggcga tcttcccgcg actgcgaacc
     2401 tggatcggcc tggcgctgct cgcgttttac gccggcgtcc tggccacgtt cgactacggc
     2461 ttccaattca acgcgatgct gaccgcgatc tattttctcc cgctcgaatc ttggatcaca
     2521 cggtgggcaa ggaagcacca gccatcaagg gcaacctaaa ctccgctaag tccgcattta
     2581 gggcagtctg cggcggatgt aatcgcccgt tgcttgacac gtcgcttaat gtgccgataa
     2641 cagttgctag ggtccgctca tagcggcccg catgctcatc agctctggct ctcgccgcgg
     2701 aaggctcttc gcatgacgac gatcgacacc agcatcgtcc cggaaccgaa tccgttcctg
     2761 gcaggcgagc tttcggccca gtcgctctac gacaaatgct ggtccctggc ccgcaacctg
     2821 tactggacgt ggcatcccga gatcatcagc ctttttcgcg acctcgaccc gatccgctgg
     2881 cggcagctcg accacaaccc gatcgccctg ctccgcgagt tcaccgccga gcgcctcgcc
     2941 cagcgggcgg ccgagatggt gctctacagc cgcatcaact atgcccaccg ccggctgaag
     3001 gaatacctgg cgaacaagca gacttgggcg gcctggaacg cgggggttct ggggggcaag
     3061 ccggtggcct atttctcggc cgagttcggc ctgcacgaat cgattccgat ctactcgggc
     3121 ggcctgggcg tgctctcggg tgaccacatc aaaagcgcca gcggcttggg cgtgaacctc
     3181 gtcgccatcg gcctgttcta cgaccagggc tatttcaagc agcagctcga cgacaccgga
     3241 tggcagcgcg aggagtacat cgacaccaag gtcgagaacg tgccgatgga gccggccctc
     3301 tctccgcagg gcaagcccat caccgtccgc gtcgataccc gcaacggccc gctcttcgcc
     3361 aaggtctgga tgatgcacgt cggccgcgtg cggctgttcc tgctcgactg cgacgtggaa
     3421 gggaacagcc cgcacgaccg cgagctgacc agccggctgt acggcggcga cgagcgggtc
     3481 cgcatccggc aagagctggt gctgggcgtc ggcggtgtca aggcgctgcg agccctgggg
     3541 atcacgcccg gcgtctatca cttgaacgaa gggcatagcg cgttcgcccc gctggaggta
     3601 atccgcgaac ggatgcacga cgacggcatg cggttcgacg acgccttccg cgaggtggcc
     3661 cgccagacgg tcttcaccac gcatacgccc gtccccgccg gccacgaccg ctttcacggc
     3721 ggcctggtcg aggagcatct cgggccgctc cgcgaccagc tcggcatcag ctacgagcag
     3781 ctgatgggcc tgggccgcgt cgagccgcag aacgaccagg aagcgttcac gatgaccgtg
     3841 atcggcctca agctctcgcg gcgggccaac gcggtgagcc agctgcacgg gcatatctcg
     3901 cggcggatgt gggcctcgct ctggccctgg cgggtcgaag aggaaattcc catcggccac
     3961 atcaccaacg gcgtgcacat cccgacgtgg ctggcccagc agatgctgca gctctacgac
     4021 cggcacttcc cggcccagtg gatcttccgc atgggcgagc cggaagtctg gcagaacatc
     4081 cacagcgtcg atccgggcga actctgggaa acgcacaacg ccctcaagaa cctgctgctg
     4141 gcgttcgtcc gccgccgcgt cagccggcag tgccgccgcc gcggcgaaag tgacgacgcc
     4201 gtcgaggccg cccgcaacgt gctcgatccc aacgtgctga cgatcggctt cggccgccgc
     4261 ttcgccacgt acaagcgggc cacgatggtc atcaacaacc tcgacctgct gaacgcgatc
     4321 tgcaacgaca aggagcggcc ggtgcagttc gtctttgccg gcaaggccca tcccaaggac
     4381 gagcccggca agcggctgat ccagacgatc gccaacctgc ggaaggaccc gcggttcgct
     4441 agccgcgtcg cctacgtcga agactacgac atgaacgtct gccggcacat gatccagggg
     4501 gtcgatgtct ggctgaacaa tccgcggcgg ccgctggaag ccagcggcac cagcggcgag
     4561 aagacggtgc tcaacggcgg actgaacctg tcgatcctcg acggctggtg ggccgaggcc
     4621 tacgacggct ccaacggctt cgcgatcggc caggggcaca gccacgtcga tgacgcgatc
     4681 acggacaagc gtgacgcgga gtcgctgtat cgcgtgatcg aggaccaggt cgtgccgatg
     4741 tattacgacc gcgacgtgga cggcctgccg cggcactggg tcaagcggat gatgaacagc
     4801 atcagcacgc tggcctggcg attcagcgcc caccgcatgg tcatggacta cacgcggctc
     4861 tgctacgtcc ccgccgccgg cggcctgagc agcgagatga cgacgcactg acggctgcgg
     4921 tcgagacggg gttagcgaca ctggcccatt cagaaaatct cggttcccaa gtatttggga
     4981 cctagaatga aagtgaggca actcgcgctt cgtttcgccg ggaaatgggc cacgcacacc
     5041 accacgatca cgatcattcc ggcacggcga gcaccacgcc ggcctttgcg atcggagttg
     5101 ccctgaacgc ggccttcgta gtcgtcgaag ccgcgtttgg attctgggcc ggctcgctgg
     5161 cgctattggc cgacgccggc cacaacctga gcgacgtgct gggcctgctg ctggcctggg
     5221 gcgcgagcta cctggcttgc cagcggccca gcaggcggcg gacatacggc ctgcgacggt
     5281 cgtcgatcat ggcggcgctg gccaacgcgc tgctcttgct cgcggccgtc ggcgcgattg
     5341 tctgggaagc cattggccgg ctcattcagc cgacgccggc cgcgcccgtg ccggtgatcg
     5401 tggtcgccgg catcggcgtg gcggtgaaca cgctgacagc gctgttgttc ttgcgcggcc
     5461 gccaccacga cctgaacgtc cgcggggcgt tcctgcacat ggccgccgac gccgccgtgt
     5521 cgctgggcgt tgtcgtcgcc ggcatcgtca tcgccttcac tggctggcag cgcgtcgatc
     5581 cgctgctcag cctgctcatc gccggcgtca ttgcctggag cacctgggac ctgctctggc
     5641 aggcggtgaa cctctcgctg gatgccgtgc cgcggggcat tgatcccgcg gccgtgcgcg
     5701 cgtacctggc ggcgctgccc ggggtgagcg aggtgcacga tcttcacatc tggggcctga
     5761 gcaccacgga gagcgccctc accgtgcatc tcatcaagcc cggcgctgcg ctcgacgacg
     5821 actggctcgc cgcggtgagc cacgagctcc acgaccgctt tggcatcggc cacgccacga
     5881 ttcaggtcga aagcggcgcc ggcagccagt cctgccgcct cgcgccggaa gacgtcgtct
     5941 agcgctcgtt cggcttcact tcgccgctcg ccggcgcggc agcgtcgggc acgaacgaga
     6001 gcccgtcgga ggctcgcagc tgaaccgtgt aggacttgcc cttttggacc ttcatcgaca
     6061 gcgccaggcc gtgggccgcg cggatgccca gctgctcggc cctctgggcg tcgttggcga
     6121 tcgcccaaaa gtcgccccag atttgcttct cgaaatcgct catcttgccg ccccgctgat
     6181 aggcccgcgg ggcggagccg acgtcgtcca gcggaaagcc gtcgatcggc tgctgcttct
     6241 cgccgaagat acggcggaag agcactagcg atgtcccccg ctccaggtcg gcggattcga
     6301 cgtatttgtc gtcgaacttc acaacccaat tatcgaggaa caccgtgtcg ccgcggatcg
     6361 ggaacagccg cggcgtgtcg atcgggtgcc cctcgtcgtt caattcaacg aattccacct
     6421 tcgtcgtcac ctccccggtc gcctcgtcct tcgtttgatc gacggccgtc agccgggcca
     6481 cgcgatgatc gaccttgagc aggctgagcg ccgtctcgag cttctcgacg tcctcgttga
     6541 gcttcgtgat ttgcgcgtcc cgctcgcgaa tctgctcggc cttggcggcg atgtcggcct
     6601 cggccgtttc gaggcgggtc ttggcgtcgg ccagggccgc ggtggccgcg gtcagctgct
     6661 cagctttctt gcgagcgtcg atttccttgg cgttgtaggt cgtgtagccg aaccagcccg
     6721 cgacgccgac gccgccgatg acgaccagcg caatcattgt ccgcaggaag ctgttgacgg
     6781 tcgaaagtcc ttccatcatg ccgggcatgt tgagtctccc gtgcgacagg ttacgcagtg
     6841 cgacaagttc acgcagtgtg gaatcatcac gctgcgcggc cgagcagcag atccaccgcg
     6901 cggccgatgt ccggctcgcg catcaggctt tcgccgacga gcatcgcctc gacgcccgcc
     6961 tcctgtaatc gtagcacgtc ggcccggctc tcgatgccac tttcacccac caaaacacaa
     7021 tccagcggca cttgcgaacg catgcgaacc gtgtgcccga gatcgaccgc gaacgtccgc
     7081 aagtcgcgat tgttgactcc caccagcgct gccccggccg ccagcacccg ctccaggttc
     7141 cgcggctcat aaaactccac cagcggcgtc attccgagct caatagcctc gttgtgcagc
     7201 ttgcgcaagt tgcagtcgtc gaggcactcg gcgatgagca gcacggc
//
LOCUS       DASRJB010000574         7745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4112503, whole genome shotgun sequence.
ACCESSION   DASRJB010000574 DASRJB010000000
VERSION     DASRJB010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112503"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFB80_15045"
     CDS             <1..1219
                     /locus_tag="VFB80_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN35143.1"
                     /translation="NLISPIALILNFLMWLPVSLAMYSGLGTLVFGPFTALGGKLLGV
                     SCDECLRLLEQLIAWGREIPYAYLWHPAPPGWLIGVFYALLAIFVAFPALRPRRQWLA
                     AIAAAWVAAALATSGIGSSMLAQVRPRPLACNFVAVGHGVSVLVELPDGKNLLYDAGR
                     LGSPLAGVRPVSAVLWSRGITHLDAVVVSHADADHFNALPDLLERFDVGVIYVSPVMF
                     ERRQPAVEELRRSIERAGVPLREIHGSQRLILGGGVKIEVLHPPRRGVLGSDNANSIV
                     LLIEYAGRRVLLTGDLESPGLDDVLAEEPLDCDVVLAPHHGSPRSHPSRFAEWSEPET
                     VVISGSQTLGDLRAVESVKHSFRLAGAEVYHTAADGCLRVEIAPRGLSARSFRPHVRA
                     VAADANANLRQPE"
     gene            1352..1591
                     /locus_tag="VFB80_15050"
     CDS             1352..1591
                     /locus_tag="VFB80_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35144.1"
                     /translation="MDAALQTNLTIAAFACSVLALFFSLLAAFPGLKVVLAAFRDGVL
                     WFALFLVLGGIGFVVWQQVQKRPQPSAAVGQAALP"
     gene            complement(1596..3035)
                     /locus_tag="VFB80_15055"
     CDS             complement(1596..3035)
                     /locus_tag="VFB80_15055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZN35145.1"
                     /translation="MSEILFHYEKIAPTTWVYVSSLLLISIFFKFNRFWSVRNLDLIL
                     LILLAPGLLMVNESRQARTAQVTAKITTVQPAPLAASALSAQDTVRLDELRRTEYFGY
                     LWLFGVSALWLIRLLLDPTMVRRPLLEPNLSNGGLIFMGCALFVFLNTNVLMSDVAPD
                     DLKGAQGAQALLDRQDRTGDKDQPEEYWRRGPGFYFLHLLPSIPTHPFVLGDHGRPER
                     VFSLVAKSMAVASQLAVVIALITIGYWHFENLKMGIGAAAMYLMLPYTAQMTGHVDHV
                     LPAALLLWAVVFYRRPLVAGILIGLALSVFYYPLFLLPLWISFYWQRGFARFVTGALG
                     SVAVMVLSLVFVSKDLASFWEKFQPMFALWIPFTSNLHGIWGLEWDPWFRLPVIVVCV
                     VLAAGLAIWPSHKNLGTLLSCSAAMMVAVQFWHGFGDGGGMYMAWYLPLTLMTVFRPN
                     LEDRVALTVLGEGWFLRRRARLAQLGRAA"
     gene            3426..4805
                     /locus_tag="VFB80_15060"
     CDS             3426..4805
                     /locus_tag="VFB80_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35146.1"
                     /translation="MNEELKEQLKAAKKKALRFAAVVAGTAPTGLLVGKKIMSPDVKA
                     LKDEKGGSKVVEGVVKGDGKNFTFQTLEKISLSEKKLKDLIESETELTVSVEWKVVEK
                     LEEIVDEAFPTELTEAYTALKLEIAQAVQAMPSRAISFTAPTEQFEKAVKKNDPEAGK
                     KALDELRTQLETAQLLIPAEQMRVTYVEKMQNRYQALFDEDVPFNLDGFLERRSVLQK
                     KWEGISKHPLSERSQATSYPIRQQEMDAEFRELLPLPERIVKDKNRRVAEFNSKIAEL
                     GEYYDAVKTALIDGKKVLSDKFEKPFSDLQRKDPSTWTSQQKFDDAEKVLVLLNQWMT
                     EATKALEKRRKEQLEKGEIVLKFDGSELDESGLKDKIKEYLPQNGHGNLNQSLNDALL
                     GRGKATTGHTGVLHASAGKPGAQGGCTLFFERDGKGNVNVVGVGQHTSSTEYFIHYGL
                     GLRGKKLSL"
     gene            4870..5337
                     /locus_tag="VFB80_15065"
     CDS             4870..5337
                     /locus_tag="VFB80_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZN35147.1"
                     /translation="MADSVFDRVCGLLTGRAIGFDVLRHAPVYTSEEAAAVRGTPLGS
                     GAKALVCKADDQFVMIVLPADRKLASKAVRKNAGVKSLRFASREEVEQLTGLAPGSIP
                     PFGSLFGLPTWCDERLSEHARINFNAGDHGISLSMNYADYLTAEQPRLGVYAE"
     gene            complement(5343..5996)
                     /locus_tag="VFB80_15070"
     CDS             complement(5343..5996)
                     /locus_tag="VFB80_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35148.1"
                     /translation="MRRTLVSLIAALVLVAIADMAAADTVQLANGDTLSGEVLSLDAK
                     ALKIKSPILGEVTIGRAKVQSITLGDAAARPAAKSTGDGLSVEGLLKQLGGVAAEAAG
                     KVNEGDLLKQLQGGIDPAELDKLKKQMPLLASPEVQAYFNKTVGGLLDGSLTIDDIRK
                     EAIRARDETKDAIKDLGPDAEKALSGYLGILDHFIKETAPKPASPPAGNTTPPAKKP"
     gene            complement(6088..7356)
                     /locus_tag="VFB80_15075"
     CDS             complement(6088..7356)
                     /locus_tag="VFB80_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35149.1"
                     /translation="MNEAGPPPEQPVASPAGGEIPAAAEAAQPLDPLTAATLTALDQA
                     PPQRTWIKSLILLAVSLGFFAMAGLFNNSPTGLAILVGVLLFHELGHYAGMRWFNYRD
                     VRMFFIPLFGAAVAGRRTSVEGYQEAIVLLLGPLPGILLGGVLGVICMFYDSEMLRSA
                     TVTLLAINGFNLLPFMPLDGGRLLHLVLFSRQRHLEALFQFVTGVLLAICGGLLGGWL
                     LAILGVFMAFGCFFTFKMSTLARELRSALALTGDTDFAAPIPRELAVPLIERVQRAFP
                     QVKQPQSLANVVRQLWERIHIRPPGMLASLGILALHGGAFLSLPAMVVIFNRPLPREA
                     TATRVDGNVVRTREVRVWGRLRSSTELDDRGRLNGRHLEYSPVTGKLLAEGNFLDGQR
                     EGIWMEYDAKGQPASMILYRRGVAIQVEQP"
     gene            7476..>7745
                     /locus_tag="VFB80_15080"
     CDS             7476..>7745
                     /locus_tag="VFB80_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN35150.1"
                     /translation="MPKDFLAGAQDEYDVVVIGSGLAGLTAANTLAKTGRSVLLLEQH
                     YQLGGMATWFKRQNGHIFDISLHGFPVGMKKTCRKYWTQEIADSIV"
BASE COUNT         1497 a         2439 c         2493 g         1316 t
ORIGIN      
        1 caacctcatt tcgccgatcg cgctgatttt gaattttttg atgtggctgc cggtatcgct
       61 ggctatgtac tccggcctgg ggacgctcgt gttcggcccc ttcaccgcgc tcggcggcaa
      121 gctgctggga gtcagctgcg acgagtgcct gcggctgctg gagcagctca tcgcctgggg
      181 ccgcgagatt ccctatgcct atctctggca ccccgcgccg cccggctggc tgatcggcgt
      241 gttctatgcg ctcttggcaa tcttcgtcgc ctttccagcg ctgcgtcccc ggcggcagtg
      301 gctggcggcg attgccgcgg cgtgggtcgc cgctgcgctg gcgacgtccg gcataggctc
      361 gagcatgctc gcgcaagttc ggccgcggcc gctggcctgc aacttcgtcg ccgtgggcca
      421 cggcgtcagc gtgctcgtgg agttgcccga cggcaaaaac ctgctctacg acgcgggccg
      481 gctcggctcg ccgctggcgg gcgtgcggcc ggtctcggcg gtgctctggt cgcgcggaat
      541 cacgcacctc gatgcagtgg tcgtctcgca tgccgacgcc gaccacttca acgccctgcc
      601 cgacctgctc gagcgtttcg atgtaggcgt gatttacgtc tctccggtca tgttcgagcg
      661 ccggcagccg gccgtggaag agttgcgaag gtccattgag cgggccggcg tgccgctgcg
      721 cgagattcac ggcagccagc ggctgatact gggcggcggc gtgaagatcg aggtgctgca
      781 cccgccgcgc cgcggcgtcc tgggcagcga caacgcgaac agcatcgtgc tgctgatcga
      841 atacgccggc cgccgcgtgc tgctcaccgg cgatctcgaa tcgcccgggc tcgacgacgt
      901 gctggccgag gagccgctgg actgcgacgt ggttctcgcg ccccaccacg gcagcccgcg
      961 cagccacccc agccggttcg ccgagtggag cgagccggag acggtcgtca tcagcggcag
     1021 ccagaccctg ggcgacttgc gcgcggtcga gagcgtcaag cactcgttcc gcctggcggg
     1081 cgcggaggtc tatcacaccg ctgccgacgg ctgcctgaga gtcgaaattg ccccgcgcgg
     1141 cctttcggcg cggagctttc gcccgcacgt tcgcgccgtc gccgctgacg cgaacgcaaa
     1201 tcttcgccag ccggaataag cggtcgcttt acggcgggaa gtagccgaat tccgcccata
     1261 gcacgccgga tttgcttgaa tcgcggctgg ccgatttgct aagacgcagg tggttgcgat
     1321 ttccacctta catctcacag gcgccacgac catggacgcc gcactgcaaa ccaacctgac
     1381 gatcgccgcc ttcgcctgct cggtgctggc cctcttcttc tcgctgctgg cggcctttcc
     1441 cggcctgaag gtcgtgctgg ccgcctttcg cgacggcgtg ctctggttcg cgctgttcct
     1501 ggtcctgggc ggcatcgggt tcgtcgtgtg gcagcaggtc cagaagcggc cgcagccatc
     1561 tgccgccgta gggcaagccg ccctgcctta gcgcgtcacg cagcgcggcc gagctgggcg
     1621 agccgcgcgc ggcggcggag gaaccagccc tcgcccagga cggtgagggc gacgcggtcc
     1681 tcgagattcg ggcggaagac cgtcatcagc gtcagcggca ggtaccaggc catgtacatc
     1741 ccgccgccgt cgccgaagcc gtgccagaac tgcacggcga ccatcatcgc ggccgagcag
     1801 ctcagcagcg tccccaggtt cttgtgcgac ggccagatgg ccagccccgc ggccagcacc
     1861 acgcaaacca cgatcaccgg cagccgaaac cacggatccc actccaggcc ccagatgccg
     1921 tggagattgc tggtgaacgg aatccagagg gcgaacatcg gctggaattt ctcccagaac
     1981 gacgccaggt ctttggagac gaaaaccagc gacaggacca tcacggcgac ggagcccagt
     2041 gcgcccgtga cgaagcgggc aaagccgcgc tgccaataaa agctgatcca gagcggcagc
     2101 aggaacagcg gatagtaaaa gaccgagagc gccaggccga tcaggatgcc cgcgaccagc
     2161 gggcgacggt agaacacgac ggcccacagg agcagcgccg ccggcagcac gtgatcgacg
     2221 tggccggtca tctgggccgt gtaaggcagc atcaggtaca tcgccgccgc gccgatgccc
     2281 atcttcaggt tctcgaaatg ccagtagccg atggtgatca gtgcgatgac gacggccaac
     2341 tgcgaggcga ccgccatcga tttggccacg aggctgaaca cgcgctccgg ccggccgtgg
     2401 tcgcccagca cgaaggggtg cgtgggaatg ctgggcagca ggtgcagaaa gtagaagccc
     2461 ggcccgcgcc gccagtattc ctccggctgg tccttgtcgc ccgtgcgatc ctggcggtcg
     2521 agtagcgctt gcgctccctg cgcgcccttc aagtcgtcgg gcgcgacgtc gctcatcagt
     2581 acattggtgt tgaggaacac gaacagcgcg cagcccatga agatcaagcc gccgttgctc
     2641 aggttcggct ccagcagcgg ccgccggacc atcgtcgggt cgagcagcag ccggatgagc
     2701 cagagcgccg aaacgccgaa cagccacagg tagccgaaat actcggttcg cctcagttcg
     2761 tccaaccgca ccgtgtcctg agccgagagc gcagacgcgg ccaggggagc cggttggacg
     2821 gtggtgatct tggcagtcac ttgcgccgta cgggcctgcc gcgattcatt caccatcagc
     2881 agtccgggag cgagcaggat cagcagaatc aagtcgagat tccgcacgct ccagaagcgg
     2941 ttgaacttga aaaagatgct gatgagcagc agcgacgaca catagaccca ggtcgtcggc
     3001 gcgatctttt cgtaatggaa taagatctcg ctcatgcggc tctactgaat gatgctccgc
     3061 atccattccg gcgcgccaac cgcgctgcgc gcgggcgcgc caactcgaca aaatagggac
     3121 ttgtggtttc tttgcaaagc ggggtgagcg gggagggatc ctagcgccgt ttgacgcaac
     3181 tactggcgca ccaaaggttt gctgccggga attcggtaat ccgtgcagtc agagttcatc
     3241 ggaataatta tctttgaact cccccttctt tgcaatggaa aaccgtgcag ccgtttggat
     3301 tccctataat cagacgcccc gggggatgag attctttcgg ccgcggggaa ggcggagcga
     3361 accgagcctg cggaccgccc cgacgattac tcgactttta ctcgcagcac ggagtatgga
     3421 ccgccatgaa cgaagaattg aaggagcagt tgaaagctgc gaagaaaaag gccttgcgct
     3481 tcgccgcggt ggtggccgga acggctccga ccggattgct ggttggaaaa aagatcatgt
     3541 cgcccgatgt caaagccctg aaagacgaga aaggcggctc aaaagttgtc gaaggggtgg
     3601 tgaagggtga cggcaaaaat ttcacgtttc agacgctcga aaagatctcc ctcagcgaaa
     3661 aaaaactcaa ggacctgatt gagtcggaga cggaactcac cgtgtcggtc gaatggaagg
     3721 tcgtggagaa actggaggaa atcgttgacg aagcgttccc gacggaactg acggaagcgt
     3781 acacggcgct caagttggaa atcgcccagg ccgtccaggc gatgccttct cgggcaatct
     3841 ccttcaccgc gcccacggag caattcgaga aagccgtcaa gaagaatgat ccggaggccg
     3901 ggaagaaggc gctcgacgaa ttacgaacgc agctggaaac ggcccagctc ctcatcccgg
     3961 ccgaacagat gcgagtcact tatgtcgaga agatgcagaa tcggtatcag gctttgtttg
     4021 acgaagacgt gccgttcaat ctggatggtt tcctggagcg tcgctccgta ctgcaaaaga
     4081 aatgggaagg aatctccaag catccgctga gcgaacgcag ccaggcgacg agctatccga
     4141 tcagacagca ggaaatggat gccgagtttc gggagcttct gcctctgccg gaacgcatcg
     4201 tcaaggacaa gaatcgtcgc gtggccgagt tcaactccaa gatcgcagag ctaggagaat
     4261 actatgacgc cgtcaaaacg gcgctgattg acggaaaaaa ggtgctcagc gacaagtttg
     4321 aaaagccctt ttccgacctg caaaggaagg atccctccac ttggacctcg cagcagaaat
     4381 tcgacgacgc ggaaaaggtg ctcgtcttgc tcaaccaatg gatgacggag gccaccaagg
     4441 cgctggaaaa acgccggaaa gaacagctgg agaagggaga gatcgttctc aagttcgatg
     4501 gctcggaact cgacgagagc ggcctgaagg acaagatcaa ggaatacctg ccgcagaacg
     4561 gtcacgggaa cttgaatcag tcgctcaacg acgcgctgct ggggcgcggc aaagccacga
     4621 cggggcatac gggcgtgcta cacgcatccg cgggaaaacc cggagcgcag ggcggctgca
     4681 cgttgttctt cgagcgcgac ggcaagggga acgtcaacgt cgtgggcgta ggccaacata
     4741 cctcctccac ggaatacttc attcactacg ggctggggct gagaggcaag aagctgtcgc
     4801 tgtaggtgag gcgacgtcct ccgattcaac agtttctaca tttgagcgac tcgattgtcg
     4861 gcagctggta tggcagacag cgtattcgac cgcgtgtgtg gcttgctcac cggccgggcg
     4921 atcggttttg acgtgctgcg gcacgcgccg gtctatacga gcgaggaagc ggcggccgtc
     4981 cgcggcacgc cgctgggcag cggggccaag gcgctggtct gcaaggccga cgaccaattc
     5041 gtgatgatcg tgctgccggc ggatcgcaag ctggcgagca aggcggtgcg gaaaaacgcc
     5101 ggcgtcaaga gcctgcggtt cgcctcgcgc gaggaagtcg agcagctgac ggggctcgcg
     5161 cccggctcga tcccgccctt cggcagcctg ttcggactgc cgacctggtg cgacgagcgc
     5221 ctgagcgagc acgcgcggat caacttcaac gccggcgacc acggcatctc gctcagcatg
     5281 aattacgccg actatctcac ggcggagcag ccgcggctgg gcgtctacgc cgagtgagcg
     5341 gcctagggtt tcttcgcggg cggcgtggtg ttgccggcgg gcggcgacgc cggctttggc
     5401 gcggtttcct tgatgaagtg atcgaggatg cccaggtatc cgctgagggc tttctcggcg
     5461 tccggcccca ggtccttgat ggcgtccttg gtttcgtcgc gggcgcggat ggcttccttg
     5521 cggatgtcgt cgatcgtcag cgagccgtcc aagaggccgc cgacggtctt gttgaaatag
     5581 gcctggactt cgggcgacgc caagagcggc atttgcttct tcagcttgtc taattcagcc
     5641 ggatcgatgc cgccctgcaa ctgcttgagc agatcgccct cgttcacttt ccccgccgct
     5701 tccgcagcga cgccgcccag ctgcttgagc aggccttcga ccgacagccc gtcgcctgtc
     5761 gatttcgccg ccggcctggc agccgcgtcg cccagcgtga tcgactgcac cttcgcgcgg
     5821 ccgatcgtga cctcgcccaa gatggggctt ttgatcttga gggccttggc gtccagcgac
     5881 aggacttcgc ccgagagcgt gtcgccgttg gccagctgca cggtatcggc cgcagccatg
     5941 tcggcgatag cgaccaggac cagggccgcg atcagactga cgagtgttcg ccgcatgatt
     6001 tcgcctcctc gattccccag agaaacgtgc cttatgttgc tcattttcgc acagccgtac
     6061 gcggaagcaa tctctgtggc gttcggctca gggttgttca acctggatgg ccacgccgcg
     6121 gcggtacagg atcatcgaag cgggctggcc cttcgcgtcg tactccatcc aaattccttc
     6181 gcgctgccca tcgagaaagt tcccttcggc gagcagtttg ccggtgacgg gcgagtattc
     6241 caggtgccgg ccattgaggc ggccgcggtc gtcgagttcc gtggagctcc gcagccggcc
     6301 ccagactcgg acttcgcggg tccgcaccac attgccgtcc acccgagtcg cggttgcctc
     6361 gcgcggcagg ggcctgttga agatcaccac catcgcggga aggctcaaaa aggccccgcc
     6421 gtgcagcgcc aggatcccca gcgacgccag catccccggc gggcggatgt gaatccgctc
     6481 ccacaactgc cggaccacgt tcgccagcga ctgcggctgc ttgacctgcg gaaacgcccg
     6541 ttgcacgcgc tcgatcagcg gcacggccag ctcgcgcggg attggcgcgg cgaaatcggt
     6601 gtcgccggtt agcgcgagcg ccgagcgcag ctctctggcc agtgtgctca tcttgaaagt
     6661 gaagaagcac ccaaatgcca tgaagactcc cagaatcgcc agcagccagc cccccaacag
     6721 cccgccgcaa atcgccagca ggacgcccgt cacgaactga aacagggcct ccaggtggcg
     6781 ctggcggctg aagagcacca ggtggagcag gcggccgccg tcgagcggca tgaacggcag
     6841 gaggttgaat ccgttgatgg ccagcagggt caccgtggcc gagcgcagca tctcgctgtc
     6901 gtagaacatg cagatcaccc ccagcacgcc gccaagcaga attcccggca gcggcccgag
     6961 cagcagcacg atggcctctt ggtagccttc cacgctcgtg cgccggccgg cgacggcggc
     7021 cccaaacagc ggaatgaaaa acatccgcac atcccgatag ttgaaccagc gcatgccggc
     7081 gtagtggccg agttcatgga acagcagcac gcccacgaga atcgccaggc ccgtcggcga
     7141 gttgttgaac aggccggcca tcgcaaaaaa gccgagcgac acagccagca ggatcaggct
     7201 cttgatccag gtccgctgcg gcggcgcttg atcgagcgcc gtcagcgtgg cagccgtcag
     7261 tggatcgagc ggctgggcgg cctcggctgc cgctggaatc tcgccgccag ccggcgatgc
     7321 cactggctgc tccggcggcg ggccggcttc attcatgacg aagtctccct taacgcgact
     7381 agtgcagatg acccgaagcg tgccgcgaaa tcaagcctcg cggcaggcgt ggtccacttg
     7441 acgagttgcc gccaagcact cagtactaac tagccatgcc caaggacttt ctggccggcg
     7501 cgcaggatga atacgacgtc gtcgtgatcg gcagcggcct cgccggattg acggccgcca
     7561 acacgctggc gaagaccggc cgcagcgtgc tgctgctcga acagcactac cagctcggcg
     7621 gcatggccac ctggttcaag cgccagaacg gccacatctt cgacatctcg ctccacggct
     7681 ttcccgtcgg gatgaagaag acctgccgta aatattggac gcaggagatc gcggactcga
     7741 tcgtc
//
LOCUS       DASRJB010000575         9940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2703477, whole genome shotgun sequence.
ACCESSION   DASRJB010000575 DASRJB010000000
VERSION     DASRJB010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703477"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFB80_15085"
     CDS             complement(<1..738)
                     /locus_tag="VFB80_15085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN35151.1"
                     /translation="MPRLLKSPPQLTAALIVRNCAAELRATLDSVNELADEIVVLDTG
                     STDDTPRIAQEFGARIFHRPWDDDYSAARNACLAHARGQWILWLDAGETLTAESVAAL
                     RGRLEQSFDVARAWLLPVVLPQAPGQLGGEQMHQLRLHPRLPGLSFAGRVRESLETAL
                     RSAALATEPLDIPIRRPAADLDAASRAHRARRNIRLADLAIAERGPSVEMHNCLGEAW
                     QRLGDSLQAAQHYTRALSLAETGSRAAL"
     gene            850..1491
                     /locus_tag="VFB80_15090"
     CDS             850..1491
                     /locus_tag="VFB80_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN35152.1"
                     /translation="MPHRHLPVAVLCSFAAALSAADEPGFTPLFDGKSLAGWEGDAKV
                     FRVVEGAIVAGSPTERIPHNEFLASKDEFGDFELRLKARLTGKGQNAGVQFRSQRVAD
                     HFEMIGYQCDMGAMQGRSIWGALYDESRRRKFLAEPADAAKLQGAFKAGDWNDLTIRC
                     EKGHVQIWVNGVQTVDYTEADDAIPRRGRLGLQIHGGEPAEAAYKDIRIRRLD"
     gene            1533..2150
                     /locus_tag="VFB80_15095"
     CDS             1533..2150
                     /locus_tag="VFB80_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN35153.1"
                     /translation="MQLRFLYFDLGNVVLRFSHQRAAEQMARVAGVSFEKVWQAVFED
                     GLEWQLERGNITREEFHARFCDAVGARPDWGALEHAASDIFELNASLVGLVGHLRATG
                     HRLGIFSNTSISHWEHCTRKFHLLNCVFDVYALSFQLRAMKPEAAAYARAAELAGVPA
                     EGIFFTDDRADNVAAALAAGWDAVLYESVSQLNAELRRRGVVLNY"
     gene            complement(2507..3619)
                     /locus_tag="VFB80_15100"
     CDS             complement(2507..3619)
                     /locus_tag="VFB80_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35154.1"
                     /translation="MTARKTRSRKVRFAVAGLGHIAQAAVLPAFAHARRKCVLAALVS
                     TDEVKLKELGEKYGIEHCYTDYDACLASGEIDAVYVAVPNHLHCEYTVRAAEAGLHVL
                     CEKPMAVTAAECSKMIHACDTAGVKLMIAYRLHFEEANLKASDMVHSGELGDVRLFSS
                     VFCLRVKEDNVRLDREQGGGTLYDIGIYCINAARYLFRAEPLEVTALSVAGNEPHLAE
                     VDEMTSAVMRFPDDRLAAFSSSFAIPEVGYYHVLGTEGNLCVDPAYDYASALEHYLTK
                     DGKTTKKRFKKRDQFAPELQYFADCVRQDKQPEPDGREGLIDVMIIEALYESARTGHS
                     VRLSLPPKADRPTLSQSREEPPVREPEEIHAESPQA"
     gene            complement(3698..3997)
                     /locus_tag="VFB80_15105"
     CDS             complement(3698..3997)
                     /locus_tag="VFB80_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35155.1"
                     /translation="MFARHRTALLILVTAALLAPAARAADKTLTGIVVSAEAGRLVIT
                     DNEGKNEQALAVPGSAKVTIDGKPAAMTDLRRGDAIAVTLDAERITQITIKRGKR"
     gene            complement(4095..4634)
                     /locus_tag="VFB80_15110"
     CDS             complement(4095..4634)
                     /locus_tag="VFB80_15110"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZN35156.1"
                     /translation="MSASVQNYDPSRPLRAGVFSTAQAAHAAVERLRAAGFAPEQITV
                     VCSDEVKEREFREFEHEQPAGSHTPTAAAAGGTVGAAVGAIAAGAAAGAAGVITGFPL
                     IAGGAGLLTGGVVGGFLGAMLTRGLEKESANFYDQAVAEGKLLVTVEAHGQRAAKELA
                     QAEQILNAAGAEPLPLESG"
     gene            complement(4791..5234)
                     /locus_tag="VFB80_15115"
     CDS             complement(4791..5234)
                     /locus_tag="VFB80_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN35157.1"
                     /translation="MSIIDAPQESATAGNQGVATPAETTPPKTRLLIVDDNEDAANTL
                     GRLLTLLGKDVRVVHGGPAAIVEVVKFQPQVVLLDLGMPGMDGIETARHIRARPAGGE
                     IVLVALTGWGQEHDRQRTEEAGFAAHLVKPVNAEQIEALLAQLVK"
     gene            complement(5412..5558)
                     /locus_tag="VFB80_15120"
     CDS             complement(5412..5558)
                     /locus_tag="VFB80_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35158.1"
                     /translation="MAVKTDTVHIKSHAKLTLGDELTLSGDQRLLSSLSVGRRAANLL
                     DVRP"
     gene            5752..7056
                     /locus_tag="VFB80_15125"
     CDS             5752..7056
                     /locus_tag="VFB80_15125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN35159.1"
                     /translation="MSRTASLEREIQEVRTGAARRAGKLEAVVEAFGDEQQSGSLDWA
                     ALRGTAEAQRLWAGIDGKSALSGHRLYAATVDDAGFIVMHTDPQRLGGRLASGWYERR
                     VPEAGPDVVWIEGGPLAGDAPAYDISLPLTAAGRWIGQYHEGLDGRWFEAQAAANHRH
                     AIVRWSWALGLIALAGAGSAGGLLYLGRREQRGRRRTERQMRERARELSQIGGGLAHE
                     VRNPLHALRINLHTLRRAMGGRGALSEEQLACTIQESDAAIDRLDALMRDLLQFTDSA
                     PGQRAELEVGREVQSAVNLLQESLRRDEIEVSTEFSPEPAVVAVDPERLRQMLHNLLS
                     FAHHRAGKLGKISVGVSRDETQASITVTDSGPPLADSQLKHLFEPFQALAASGSGLGM
                     TLVQTFAEASGGHVSLERPGPTGSRLRVCLPLAPSNSMGVAQ"
     gene            7053..8456
                     /locus_tag="VFB80_15130"
     CDS             7053..8456
                     /locus_tag="VFB80_15130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN35160.1"
                     /translation="MKPKHKVLVVEDKQHERLAIARLLWQEDYEVTLAENPQQASTFL
                     DEDIDLVITDLKMGEHSGLDLLRLWKKRHPATPFVLITAHGEVNSAVEAMKLGAEDYL
                     TKPVNPDELLMLIARCIEFRRKDETIRHLQERLDERLGFEKMIGSSQEILDVFEQARM
                     AAQADCTVLVTGESGTGKELVAEALHHNSPRKDGPFMTVNMAAVPEHLVESELFGHVK
                     GAFTGALAARTGRFEAAHNGTLFIDEIGDFALSSQAKLLRVLENHMITPVGSNDDKQV
                     DVRAIAATSRRLEEMVKSGAFREDLYYRLNVVTIHLPALRERPEDIPLLAVHFLRSFG
                     EAHAKTDLQIDPDLMEYLENYAWPGNVRQLRNAMENMVVLARGSVLTLDNLPASLEGD
                     PLAGTSAPPPPKSLVALQRAAVERALADSHGHRTRAAEALGISVRTLQRKLKAWGLEN
                     SDPQHSGESLLHRTAER"
     gene            8531..>9940
                     /locus_tag="VFB80_15135"
     CDS             8531..>9940
                     /locus_tag="VFB80_15135"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZN35161.1"
                     /translation="MPALQDPPLEATELAALLDALTALKKGRRGVRLPQNWTGVAGKV
                     ADAFNEVVELNEQMAGELGRLSRVVGKDGKLGQRLSLGKVSGFWEESVDSVNELIGDL
                     VHPTSETARVIGAVAQGDLLQMMALEVGGRPLQGEFLLTARTVNRMVDQLRSFASEVT
                     RVAREVGTEGKLGGQAQVKGVAGTWKDLTDSVNSMAGNLTDQVRNIAAVTKAVAAGDL
                     SKKSTVDVKGEILELKNTVNTMVDQLSSFASEVTRVAREVGTEGKLGGQADVPGVAGT
                     WKDLTDNVNSMASNLTNQVRNIADVTKAVQAGDLSRKITVDVRGEILELKNTVNTMVD
                     QLRSFASEVTRVAREVGTEGKLGGQADVPGVAGTWKDLTDNVNFMASNLTGQVRNIAD
                     VTKAVQAGDLSRKITVDVKGEILELKNTFNTMVDQLSSFASEVTRVAREVGTEGKLGG
                     QADVPGVAGTWKDLTDNVNS"
BASE COUNT         1699 a         3247 c         3369 g         1625 t
ORIGIN      
        1 cagcgccgcg cgcgaacccg tttcggcgag agacaaggcg cgggtgtagt gctgcgccgc
       61 ctggagcgag tcgcccagcc gctgccaggc ttcgccgagg cagttgtgca tttcgacgga
      121 tggtccccgc tccgcgatcg ccaggtcggc caggcggatg ttgcgccgcg cccggtgggc
      181 ccgcgatgca gcgtccaggt cggcggccgg gcgtcgaatg ggaatatcga gcggctcggt
      241 tgccagtgcg gccgaacgca gcgccgtttc gagcgattcg cgcacgcggc cggcaaagct
      301 cagccccggc aatcgcggat gcaggcggag ctggtgcatt tgctcgccgc ccagttggcc
      361 cggcgcctgc ggcaaaacga caggcagcag ccaggcccgc gcgacatcga aagactgctc
      421 cagccggccg cggagcgctg cgaccgactc ggcggtaagc gtctcgcccg cgtcgagcca
      481 caaaatccac tggccgcggg cgtgggcgag gcaggcgttg cgggccgcgg aataatcgtc
      541 gtcccacggc cggtgaaaga tgcgggcgcc gaactcctgc gcaatccgcg gcgtgtcgtc
      601 ggtcgaaccc gtgtcgagaa cgacgatctc gtcggccagc tcgttgaccg aatccagcgt
      661 ggcccgcagc tccgccgcgc agttgcggac gatcagcgcg gcagtaagct gcggcggcga
      721 tttcagcagg cggggcacgg gacgcctcct gggccggcgg accttggcgg cggagtgtag
      781 ccgcgccttt ggcctttgac ctaggccgga acggcgtgct agcttgcacg cctcctggga
      841 gctgccgata tgccgcaccg ccatttgccc gtcgctgttt tgtgctcctt cgccgccgcg
      901 ctgtccgccg cggatgagcc gggcttcacg ccgctgttcg atggcaagtc cctggcgggc
      961 tgggaaggag acgccaaggt ctttcgagtg gtggaaggcg cgatcgtcgc cggctcgccg
     1021 accgaaagaa ttccgcacaa cgaattcctg gcgtcgaagg acgaattcgg cgacttcgag
     1081 ctgcggctga aggcgcggct gaccggcaag ggccagaacg ccggagtgca gttccgcagc
     1141 cagcgcgtcg ccgaccactt cgagatgatc ggctaccagt gcgatatggg cgcgatgcag
     1201 gggcggtcga tctggggcgc gctgtacgac gaatcgcggc ggcgcaagtt tctggccgag
     1261 ccggcggacg cggcgaagct gcaaggggcg tttaaagccg gcgactggaa cgacctgacg
     1321 atccgctgcg agaaaggtca tgtgcaaatc tgggtgaacg gcgtgcagac ggtcgattac
     1381 acggaggccg acgacgcgat tccgcggcgc ggccgcctgg gcctgcaaat ccacggcggt
     1441 gagccggcgg aagcggccta caaggacatc cgcatcaggc ggctcgactg actggccttg
     1501 cgcgaagctg cgcggcgtgg ttcaatagcc ggatgcagct gcgatttctg tatttcgatc
     1561 tgggcaacgt ggtgctccgc ttctcgcacc agcgggccgc ggaacaaatg gcccgggtgg
     1621 cgggcgtttc gttcgaaaaa gtctggcagg ccgtcttcga ggacggcctg gagtggcaac
     1681 tcgaacgggg aaacatcacc cgcgaggagt tccacgcccg cttctgcgac gcggtcggcg
     1741 cgcggcccga ctggggcgcc ctcgagcacg ccgccagcga catcttcgag ctcaacgcat
     1801 cgctcgtggg cctcgtggga cacctccggg ccaccggcca tcggctgggt atcttctcga
     1861 acactagcat ctcccactgg gaacactgca cgcgcaagtt ccacctcttg aactgcgtgt
     1921 tcgacgtcta cgcgctgagt tttcagctgc gggccatgaa gcccgaggcc gccgcctatg
     1981 cccgcgcggc cgaattggcc ggcgtgccgg ccgagggcat cttctttacc gacgaccggg
     2041 ccgacaacgt cgccgccgcc ctcgcggccg gctgggacgc cgtgctgtac gagtcggtga
     2101 gccagctgaa cgccgagctg cggcgtcgcg gcgtcgtgct caactattag ccattagcgg
     2161 cgattttttc cgccgccgaa tcggaacgcc tatcgtccta agcccttggg aaataatgcc
     2221 ttagatcaat tccataggcc ctttgtgtac attaggatta taagcattag aaaggttagt
     2281 aggcggggta ggcgggcgat ttctcgcggg ttggcgacgg tcttgcgtct cccgcgccgc
     2341 ggggccggtg atgcggttgc tgcattccgc gcggacccga ggcgcacccc cgccccgaga
     2401 gtcaggtgcc gtcgctgcct cacgtgatcc ctagtgaaca gattatagca aatgccccct
     2461 gcctcgagcc ctcagtcgag gggtgagcct gaaaagaatc tctggcctac gcctgcggcg
     2521 actcggcgtg gatttcctcc ggctcgcgga ccgggggctc ttcgcggctc tgcgagagcg
     2581 tcggccggtc ggctttgggc ggcagagaca gacgcacgct gtgcccggtg cgggccgatt
     2641 catagagggc ttcgataatc atgacgtcga tcagcccctc gcggccgtcg ggctcgggct
     2701 gcttgtcctg gcggacgcag tcggcgaagt attgcagctc cggagcgaac tgatcgcgct
     2761 tcttgaagcg ctttttggtc gtcttgccgt ccttggtcag gtagtgctcc agggccgagg
     2821 catagtcgta ggccggatcg acgcagagat tgccctccgt gcccagcacg tgataatagc
     2881 cgacctcggg aatggcaaag ctggagctga aggcggccag ccggtcgtcc ggaaaccgca
     2941 tgacagccga ggtcatttcg tccacttcgg ccaaatgtgg ttcgttgccc gccaccgaga
     3001 gcgcggtcac ttccagcggc tccgcgcgaa acagatagcg ggcggcgttg atgcagtaga
     3061 tgccgatgtc gtagagcgtg ccgccgccct gctcgcgatc cagccggacg ttgtcctcct
     3121 tgacccgcag gcagaagacc gagctgaaca gccgcacgtc gcccagctcg ccggaatgga
     3181 ccatgtcgct ggccttgagg ttggcctctt cgaagtgcag gcggtaggcg atcatcagct
     3241 tgacgccggc ggtgtcgcag gcgtggatca tcttcgagca ctccgcggcg gtgacggcca
     3301 tcggcttttc gcagaggacg tgcaggccgg cctcggctgc ccgcactgta tattcgcagt
     3361 gcaggtggtt cgggaccgcg acatagaccg cgtcaatttc gccgctggcc agacaggcgt
     3421 cgtaatcggt gtagcagtgc tcgatgccgt atttctcccc cagctccttc agcttgacct
     3481 cgtcggtcga gacgagggcc gcgagcacgc acttccgccg cgcgtgcgca aacgccggca
     3541 gcacggccgc ctgcgcgata tgaccgagtc cggcgacggc gaagcgaacc tttcggctgc
     3601 gggtcttgcg agcagtcatg gcgttttccc agcgggttta aggtccgacc cgcggcgatg
     3661 ggtaccgcgg gccggcgatg cggccgcgcg atttgcatca gcgcttgccg cgcttgatcg
     3721 tgatctgcgt gattcgctcg gcgtccagcg tcacggcgat ggcgtcgccg cggcggaggt
     3781 ccgtcatcgc ggccggcttg ccatcgatcg tgaccttggc cgagccgggc acggccaggg
     3841 cctgctcgtt cttgccttcg ttgtcggtga tgacgagccg ccccgcttcg gccgaaacga
     3901 cgatgcccgt cagtgtcttg tccgcggcgc gggccgccgg cgcgagcagc gccgcggtga
     3961 cgagaatcaa gagcgcggta cggtggcgcg caaacatgga cgcctcccct gtcggcatgg
     4021 agtttcacga gcccgagact tgcccggcaa tcaaaggcag cactttccgt tccggcgatt
     4081 ttcgccagcg ccggctagcc ggactcgagc ggcaggggct ctgcgccggc ggcgttcaga
     4141 atctgctcgg cctgggccag ctccttggcg gcccgctggc cgtgagcctc gacggtgacg
     4201 agcagcttcc cttccgcgac ggcctggtcg tagaaattgg ccgattcctt ttcgagtcca
     4261 cgcgtgagca tcgcgccgag gaagccgcct acgacgccgc ccgtgagcag gccagcgccg
     4321 ccggcgatca gcggaaaacc cgtgatgacg ccggctgcgc ccgcggctgc tccggcggca
     4381 attgcgccca ccgcggctcc gaccgtgccg cccgcggctg cagctgtcgg cgtatggctg
     4441 cccgccggct gctcgtgctc gaactcgcga aattcccgct ctttcacttc gtccgagcag
     4501 accacggtga tttgttctgg tgcgaatccc gcagcacgca gccgctcgac ggccgcgtgg
     4561 gccgcttggg ccgtggaaaa gactccggcg cggagcggcc gggaggggtc gtagttctgc
     4621 acggatgccg acatcgattg cctccactgg gaagcaatcg caaagcgggt gccaggatcg
     4681 gcgatcttat aggttgtcga ttgcgtcttt ttgactaacc cgtagccgga ggcgcaggct
     4741 ttttcccgat agccgccgtc gcctccggct ccgggttaaa cgaagctcgg ctatttcacc
     4801 agctgtgcga gcagggcctc gatttgttcg gcgttgactg gcttgaccaa atgggcggcg
     4861 aaacccgcct cctcggttcg ctggcggtcg tgctcctgcc cccaaccggt gagggcgact
     4921 agcacgatct cgcctcccgc agggcgagct cgaatgtgcc gcgcggtctc gatcccgtcc
     4981 atccccggca tgcccagatc gagcaagacg acctgcggct gaaacttcac gacctcgacg
     5041 attgccgccg gtccgccgtg aacgacgcgg acgtccttgc ccagcagcgt gagcagccgc
     5101 ccgagcgtgt tcgcggcatc ctcattgtcg tcaacgatca ggagtctcgt tttggggggc
     5161 gttgtttcgg ctggcgtcgc gactccctgg tttcctgcag tcgcggactc ctggggggca
     5221 tcgatgatac tcatttcatc tcaccttgga tcagccagct caaagtgatt cgccggggca
     5281 tccttacaag cgaacccatc aaaaggtgca attaatatgc cgctaatact aagctcacgg
     5341 cacgcgaatt gcgatgtcgc acggccgcga tttggctccc ttccagctag cgttgacggt
     5401 gacatgacgc gctacggtcg cacgtcgagc aggttggctg cgcgcctgcc aacgctgagc
     5461 gacgacagca gacgctgatc accagacagc gtcaattcgt cgccgagcgt caacttggca
     5521 tgcgatttga tatgcacagt gtcggtcttg acggccaacc aacgcaggag tcccagcgct
     5581 cgatgagccg cgtgcctttg tggcgggatc tgcccgccga gccagctatc cttgatcccg
     5641 ccgcgagata gagcgaggac ggattggacg ctgtgggcgt acggcttggt tctcgcgccg
     5701 gcggccttct tgcttgcgct gggcctctgg aacgcctctg ccgatttgca aatgagccgc
     5761 accgcttcac ttgagcgcga aatccaggag gtccggaccg gcgcagcgcg gcgggcgggc
     5821 aagctggagg cagtcgtgga ggcgtttggc gacgaacagc agtcgggctc tctcgattgg
     5881 gcggcgctcc gcggcactgc cgaagcgcag cggctgtggg ccggcatcga tggcaagtcc
     5941 gcgctcagcg gccaccgcct gtacgcggcg acggtggacg acgcggggtt catcgtcatg
     6001 cacaccgacc cgcagcgcct gggcgggcga ctggcgtcgg gctggtacga gcggcgcgtg
     6061 cccgaagccg gcccggacgt ggtgtggatc gaaggggggc cgctcgcggg cgacgccccg
     6121 gcctacgaca tcagcctccc actcacggcc gccggtcgct ggatcggcca gtatcacgag
     6181 gggctcgacg gccgctggtt cgaagcccag gccgccgcga atcaccggca cgccatcgtc
     6241 cgctggagct gggccctggg gttgatcgcg ctcgccgggg ccggatccgc gggcgggctg
     6301 ctctatctgg gccggcggga acaacgcgga cgccggcgga cggagcggca gatgcgcgag
     6361 cgggcccgcg agctgtcgca aatcggcggc ggactggcgc acgaagtccg caatccgctc
     6421 cacgccctgc ggatcaacct gcacactctg cggcgggcca tgggcggccg cggcgcgctg
     6481 agcgaggagc agctggcgtg caccatccag gaatccgatg cggcgatcga ccgactcgac
     6541 gcgctgatgc gcgacttgct gcagttcacg gactctgcgc cggggcagcg ggccgagctg
     6601 gaagtcggcc gcgaagtgca gtcggccgtc aacctgctcc aagaaagctt gcgccgcgac
     6661 gagatcgagg tctcgaccga gttctcgccg gagccggcgg tggtcgccgt cgatcccgag
     6721 cggctgcggc agatgctgca caacctgctg tcgttcgcgc accaccgcgc cggaaagctc
     6781 ggcaaaatct cggtcggcgt gagccgcgac gagacgcaag ccagcatcac cgtcaccgac
     6841 agcggcccgc cgctggccga cagccaactg aaacacttgt tcgagccgtt ccaggccctg
     6901 gccgcgagcg gctccggcct gggaatgacc ctggtgcaga ccttcgcgga agccagcggg
     6961 ggccacgtgt ccctggagcg ccccgggccg accggcagtc ggctgcgggt ttgccttccg
     7021 cttgccccct ccaattcgat gggagtcgcg cagtgaaacc gaagcacaaa gtcctggtgg
     7081 tcgaagacaa gcagcacgaa cggctggcga tcgcccgctt gctctggcaa gaggattacg
     7141 aggtgacgct ggccgagaac ccgcagcagg ccagcacctt cctcgacgag gacatcgacc
     7201 tggtcatcac cgacctcaag atgggcgagc acagcgggct cgacctcttg cggctgtgga
     7261 agaagcggca cccggcgacg ccgttcgtgc tcatcacggc ccacggcgag gtgaattcgg
     7321 cagtcgaggc gatgaagctc ggcgcggaag attacctgac caagcccgtc aatcccgacg
     7381 agcttttgat gctgatcgcc cgctgcatcg agttccgccg caaggacgag accatccgcc
     7441 acctccagga gcggctcgac gagcggctgg gcttcgagaa gatgatcggc tcctcgcagg
     7501 agatcctcga cgtcttcgaa caggctcgca tggccgccca ggccgactgc accgtgctgg
     7561 tcaccggcga atcgggcacc ggcaaggagc tggtcgccga ggcgctgcac cacaacagcc
     7621 cgcggaaaga tgggccgttc atgaccgtca atatggcggc cgtgcccgag catctggtcg
     7681 agagcgagtt gttcggccac gtcaaaggcg cgttcaccgg cgctctggcc gcccgcaccg
     7741 ggcggttcga agccgcccac aacggcacac tgtttatcga cgagatcggc gatttcgccc
     7801 tctcttcgca ggcgaagctg ctccgcgtgc tggaaaacca catgatcacg cccgtgggca
     7861 gcaacgacga caagcaggtg gacgtgcggg ccatcgccgc caccagccgc aggctggaag
     7921 agatggtcaa aagcggcgcg tttcgggagg acctgtatta ccggctgaac gtcgtcacga
     7981 tccatctgcc ggcactgcgc gagcggccgg aggacatccc gctgctagca gtgcacttcc
     8041 tgcggtcgtt cggcgaagcc cacgccaaga cggacctgca gatcgatccg gacctgatgg
     8101 agtatctgga gaactacgcc tggccgggca atgtgcgcca gctgcgcaac gcaatggaga
     8161 acatggtcgt gctagcccgc ggcagcgtct tgacgctgga caacctgccc gcctcgctag
     8221 aaggcgatcc gctggcggga accagcgctc cgccgcctcc caaatcgctg gtggccttgc
     8281 agcgggcggc ggtcgaacgg gccctggccg acagccacgg ccaccgcacc cgcgccgccg
     8341 aagccctcgg catttccgtc cgtacgctac agcgcaagct gaaagcctgg ggtctggaaa
     8401 actcagaccc ccagcattcc ggcgaatcgc tcttgcatcg tacggcggaa cgctaagact
     8461 gatggatcgg ctgcctgcct tttcctgagc ctgccggatt cccgcctact ctcccacgag
     8521 ggaacgaacc atgcccgccc tgcaagaccc gccgctggaa gccacggaac ttgccgctct
     8581 cctggacgcg ctgacggcgc tcaagaaggg gcggcgcggc gtccgcctgc cgcagaactg
     8641 gacgggggtt gccggcaagg tggccgacgc cttcaacgaa gtcgtcgagc tgaacgagca
     8701 gatggcgggc gagctcggcc ggctcagccg cgtcgtcggc aaggacggca agctgggcca
     8761 gcggctctcg ctgggaaagg tctcgggctt ctgggaggaa tcggtcgatt ccgtcaacga
     8821 gctgatcggc gacctggtgc atccgacgag cgagacggcc cgcgtgatcg gcgcggtggc
     8881 ccagggggac ctgttgcaaa tgatggccct ggaggtcggc ggccggccct tgcagggcga
     8941 attcctgctc acggcccgca ccgtcaaccg gatggtcgat cagctccgct cgttcgccag
     9001 cgaagtgacc cgcgtggccc gcgaggtcgg caccgagggc aagctcggcg gccaggctca
     9061 ggtcaagggc gtggccggta cctggaagga cctgacggac agcgtcaaca gcatggccgg
     9121 caacctgacc gaccaggtcc gcaacatcgc agccgtgacg aaggccgtgg ccgccggcga
     9181 cctgagcaaa aagagcacgg tcgatgtgaa gggggaaatt ctggagctga agaacaccgt
     9241 caacacgatg gtcgatcagc tcagcagctt tgccagcgaa gtgacccgcg tggcccgcga
     9301 ggtcggcacc gagggcaagc tcggcggcca ggccgacgtg cccggcgtcg ccggcacctg
     9361 gaaggacctg accgacaacg tcaactcgat ggccagcaac ctgacgaacc aggtccgcaa
     9421 catcgccgac gtgaccaagg ccgtgcaggc cggcgacttg tcgcggaaga tcacggtcga
     9481 tgtccgcggc gaaattctgg agctgaagaa caccgtcaat acgatggtcg atcagcttcg
     9541 ctcgttcgcg tcggaagtga cccgcgtggc ccgcgaagtg ggcacggagg gcaagctggg
     9601 cggccaggcc gacgtgcccg gcgtcgccgg cacctggaag gacctgaccg acaacgtcaa
     9661 cttcatggcc agcaacctga ccggccaggt tcgcaacatc gccgacgtga cgaaggccgt
     9721 gcaggccggc gacctgtcgc gcaagatcac ggtcgatgtg aagggggaaa tcctggagct
     9781 gaagaacacc ttcaacacga tggtcgatca gctcagcagc tttgccagcg aagtgacgcg
     9841 cgtggcccgc gaagtcggca ccgagggcaa gctgggcggc caggccgacg tgcccggcgt
     9901 cgccggcacc tggaaggacc tgaccgacaa cgtcaacagc
//
LOCUS       DASRJB010000576         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1575175, whole genome shotgun sequence.
ACCESSION   DASRJB010000576 DASRJB010000000
VERSION     DASRJB010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575175"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1535
                     /locus_tag="VFB80_15140"
     CDS             282..1535
                     /locus_tag="VFB80_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HZN35162.1"
                     /translation="MQIVVFEDEQVPRLYPLTVGRAAYALSCGSYRLIDWLGRLAAET
                     GAVLQGAVRPHLSEIQRLDFPQIGSAAAASDSPVLLVNARLVPSVTSYRELKQLIAGQ
                     QAVVIDEQSVAAALITAGGPAPPPADKFEQWTGYLHSLPGKLQRAEAKLPLFHYPHDL
                     VRQHLQILGENLAERLKEGRFREVADGVFAAAGAQLGQYCVSDTSKGPVLLDEGATVG
                     PYSFLRGPAYLGKKSRVIEHSAIKDFVALGHTTKIGGEIEASIVEPFTNKQHHGFLGH
                     SYLGSWINLGAGTSNSDLKNTYGNVKMDYRGESVASGMQFVGCFMGDYAKTAINTGIF
                     TGKTIGCCSMVYGFVTTNVPSFVNYARLFGQVTELPPEVMIATQQRMFARRNVEQRDC
                     DIRLLHAMFDLTRHERQLAGEPLAL"
     gene            1669..>2116
                     /locus_tag="VFB80_15145"
     CDS             1669..>2116
                     /locus_tag="VFB80_15145"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN35163.1"
                     /translation="MIQQRRAATIADLYRVQEKAELVNGEIVIMSPASAGHGRVAGRI
                     FRSLLEYEERTLSGQAIPDNVGFIVDLPNRQSFSPDAAYFFGAINEQEFVDGAPSFAA
                     EVRSPEDFGPAAERELAAKRADYFAAGTQVIWDVDMIREKAIRVYRA"
BASE COUNT          404 a          694 c          646 g          372 t
ORIGIN      
        1 ccgatccgcc cgctgccggc ggcgcgggag tagggaggtt accattcgtg gccaatagta
       61 tacaattgag tgagagagtt ggcattggcc aatgacgccc gaatctcgct tgcgcgcata
      121 atgcgcataa tcgccgcgaa gtcatttgca gcataacctg tttctgtgaa acgacttacg
      181 tcaatgattt cggcgcgaca atttatttca atatgcgcgc atgaccgcct gcggaccgtc
      241 gccggactgc ggcgggctag acttgcgacg gcaggcccac gatgcaaatc gtcgttttcg
      301 aggacgagca ggtgccgcgg ctctacccgc tgacggtggg ccgggcggcc tatgcgcttt
      361 cctgcggcag ctatcggctg atcgactggc tgggccgcct cgcggcggaa accggtgcgg
      421 ttttgcaggg cgccgtgcgg ccgcacttga gcgaaatcca gcggctggat tttccgcaga
      481 tcggttcagc cgcggcggcg agtgactcgc cggtgctgct cgtgaacgct cggctggttc
      541 cgtcggtcac ctcgtatcgc gagctgaagc aactgatcgc gggccagcag gccgtcgtta
      601 tcgacgagca atccgtcgca gcggccctga tcaccgctgg cggtcccgct ccgccgcccg
      661 cggacaagtt cgagcaatgg accggttacc tgcattcact gccgggcaag ctgcagcggg
      721 ccgaagcgaa actgccgctg ttccactatc cgcacgacct ggtgcggcag cacttgcaga
      781 ttctcggcga gaacctggcg gagcggctga aggagggccg ctttcgcgaa gtcgccgatg
      841 gagtctttgc ggcggccggc gcgcagctcg gccagtattg cgtgagcgac acgagcaaag
      901 gtccggtgct gctcgacgaa ggcgccacgg tcgggcccta cagtttcctg cgcggcccgg
      961 cctatctggg aaaaaagtcg cgggtcatcg agcactcggc gatcaaggat ttcgtcgccc
     1021 tcggccacac gaccaaaatc ggcggcgaga tcgaggcctc gatcgtcgag ccctttacca
     1081 acaagcaaca ccacggattc ctcggccact cctatctcgg cagctggatc aacctcggcg
     1141 ccggcacgag caacagcgac ctcaaaaaca cctacggcaa cgtcaagatg gactaccgcg
     1201 gcgaatcggt cgccagcggc atgcagttcg tcggctgctt catgggcgac tatgccaaga
     1261 cggccatcaa caccggaatc ttcaccggca agacgatcgg ctgctgctcg atggtctatg
     1321 gcttcgtgac caccaacgtg cccagcttcg tcaattacgc ccgcctgttc ggccaggtca
     1381 ccgagctgcc gccggaggtg atgatcgcca cgcagcagcg gatgttcgcc cgccgcaacg
     1441 tcgagcaacg cgactgcgac atccgcctcc tgcacgccat gttcgacctc acgcgccacg
     1501 agcgccaact cgccggcgaa ccgctcgcgc tgtagggtgg gaccagcgag tccgcgagcg
     1561 ccggcccacc tcaatactgc gccaagtgaa tggtgggccg gcggccgcga cggccttgtc
     1621 ccaccctacc gccgcgagca tgttacgatg aacgaaagga gcacgaccat gattcaacaa
     1681 cggcgcgcgg ccacaatcgc cgacctttat cgcgtccagg agaaggccga gctggtcaat
     1741 ggggaaatcg tcatcatgtc gccagccagc gcagggcacg gccgagtcgc cggcaggatt
     1801 tttcgcagtc tgcttgaata cgaggagcgc acgctcagcg ggcaggccat tcctgacaac
     1861 gtcggtttca tcgtcgatct acccaaccgc cagtcgtttt cgccagacgc tgcttatttc
     1921 tttggcgcca tcaacgaaca ggagttcgtc gatggcgctc cctcattcgc cgccgaagtc
     1981 cgcagccccg aagacttcgg ccctgccgcc gaacgagagc tggccgccaa acgcgccgac
     2041 tacttcgccg ccggtacaca ggtcatctgg gacgtcgaca tgattcgcga gaaagccatc
     2101 cgcgtctatc gcgccg
//
LOCUS       DASRJB010000577         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_449108, whole genome shotgun sequence.
ACCESSION   DASRJB010000577 DASRJB010000000
VERSION     DASRJB010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449108"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VFB80_15150"
     CDS             complement(<1..1485)
                     /locus_tag="VFB80_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN35164.1"
                     /translation="MILRLGSLAAVLLACGYCTAAEAQPVSFEQHVRPILKLHCFQCH
                     GEEDEKQANLDLRQTRLIIKGGDSGPAIVPGNAARSLLIEKLAASEMPPDGKGKPLSA
                     EQLATIRTWIDQGSKTARPEPETLSAVTDAERSFWSFQPLKRPPLPAVREAALLHSPI
                     DAFLSEKLEQRGLAFSPAADRAALIRRASFDLHGLPPTPEEVAVFSADDSPDAWERLL
                     DRLLASPRYGERWGRHWLDVAGYADSDGYTEKDPERKYAFKYRDYVIRSLNADQPWDA
                     FLREQLAGDEMLTQPFANLTAEQADLLAATGFLRMAPDGTADGNVDQAAARNDVVAET
                     LKIVSSSLLGLTVGCAQCHDHRYDPIPQVDYYRLRAIFEPALDVKGWRTPQGRLVSLW
                     TDAVREQAAAVDADLKKLTDEPQAEMDRIVEAIFNDEVAKLPEEQRELARQARAADAK
                     ERTSEQKQLLKNHPSLNVSRGSAYLYDRKRVDALNKDFDARTKEL"
     gene            complement(1567..>2954)
                     /locus_tag="VFB80_15155"
     CDS             complement(1567..>2954)
                     /locus_tag="VFB80_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN35165.1"
                     /translation="GLGIVIAAMLPCWAQSQPNLVVFIADDLGGLDTTPYGSKEVRTP
                     IFDRLAREGLKFTQAFVASPSCAPSRAALLTGLMPARNGAEPNHTRPRAEIKKLPAYL
                     QELGYEVASFGKVAHYNHGSYYGIDYVAGEGGGNYTGIGLAVDWLKQRKSDKPLCLMV
                     GTNWPHVPWPRETPGYEPSKTILPPTFVDTPETRLWRSRYCAAVTKADDDLGSVLEAV
                     GKSLDPKNTFVFFTSDHGAQWPFGKWNLYDSGIRVPLIVTGPGIKAGASADAMVSWID
                     LLPTLVELAGGKPPAEIDGRSFAGVLLGKADKHREEIFATHSGDGRFNVYPIRAVRDG
                     RWKYILNLHPEYQHATHINRAATGDGLSYWNSWEAAAKSDPRAAAIVQRYKQRPREEL
                     YDLSADPHEQTNLAAEPQHAQRLAALRDKVAAWMKSQGDAGRVFNEPLLLGQEATPIQ
                     PPTKAKSSKDK"
BASE COUNT          441 a          969 c          993 g          551 t
ORIGIN      
        1 caactccttg gtgcgggcgt cgaagtcctt gttgagggca tcgacccgct tgcggtcgta
       61 gagataggcg ctgccgcggc tgacattgag gctgggatgg ttcttgagca gctgcttttg
      121 ctcgctggtg cgctccttgg cgtccgccgc ccgagcctgg cgggccagct cgcgctgctc
      181 ctcaggcagc ttcgcgacct cgtcattgaa aatcgcctct acaatccggt ccatctccgc
      241 ctgcggctcg tcggtgagct ttttcaagtc cgcatcgacg gcggccgcct gctcccgcac
      301 ggcgtcggtc cagagcgaga ccagccgccc ctgcggcgtg cgccagccct tcacgtcgag
      361 ggccggctcg aaaatcgccc gcaggcggta atagtccacc tggggaatcg gatcgtagcg
      421 atggtcgtgg cactgggcgc agccgacagt gaggcccagc agcgatgagc tgacgatctt
      481 tagcgtctcg gcgacgacgt cgttgcgggc ggcggcctga tcgacgttgc cgtcggccgt
      541 gccgtcgggc gccatccgca gaaagcccgt cgccgccagc aggtccgcct gctctgcggt
      601 caggttcgca aacggctgcg tcagcatttc gtcgcccgcc agctgctcgc gcaggaaggc
      661 gtcccacggc tggtcggcgt tcagcgaccg gatcacgtag tcgcgatact tgaaggcgta
      721 ttttcgctcc gggtcttttt ccgtgtagcc gtcgctgtcg gcatagccgg cgacgtcgag
      781 ccagtgccgg ccccaccgtt cgccgtaccg aggcgaggcg agcagccgat cgagcaggcg
      841 ctcccaggcg tcgggcgaat cgtcggcgct aaagaccgcg acttcctcgg gcgtcggcgg
      901 caggccgtgc aaatcgaacg aggcccggcg gatcagtgcg gccctgtcgg ctgccggcga
      961 aaacgcgagc ccacgctgct ccagcttttc cgacaggaag gcgtcgatcg gcgagtgcag
     1021 cagcgccgcc tcgcgcaccg cgggcagcgg cggccgcttc aacggctgaa acgaccagaa
     1081 gctgcgttcg gcgtcggtga cggctgagag cgtttcgggc tccggccggg cggtcttcga
     1141 gccctggtcg atccaagtgc ggatcgtcgc cagttgctcg gccgacagcg gcttgccctt
     1201 gccgtccggc ggcatttcgc tcgcggccag tttctcgatg agcagactgc gggccgcgtt
     1261 tcccggcacg atcgccgggc cgctgtcgcc gcccttgatg atcagccgcg tttgacgcag
     1321 gtcgagattc gcctgcttct cgtcttcctc gccgtggcac tgaaagcagt gcagcttgag
     1381 gatcggccgc acgtgctgct caaagctcac cggctgagct tctgcggcgg tgcaataacc
     1441 gcaggcgagc aggacagcgg cgaggcttcc caagcggagg atcatgggcg ggattgcagg
     1501 cgggacaacc gggcgggtag tcgcattctc gccgtggcgg cgggcgtttg caacccaaag
     1561 cgtgtgctac ttgtctttgc tcgatttggc tttcgttggg ggctggatcg gcgttgcctc
     1621 ctggccgagc agcagcggtt cgttgaagac gcggccggcg tcgccttggg atttcatcca
     1681 ggcggcaacc ttgtcgcgga gtgcggccag ccgctgggcg tgctgaggct cggcggccag
     1741 gttcgtctgc tcgtgcggat cggcggaaag gtcgtacagc tcctcgcggg gccgctgttt
     1801 gtagcgctgg acgatcgccg ctgctcgcgg gtcactcttc gccgctgcct cccaggaatt
     1861 ccagtaggac aggccgtcgc cggttgccgc gcggttgatg tgcgtggcat gctggtactc
     1921 gggatgcagg ttgaggatgt atttccaccg cccatcgcgg acggcccgga tcggatagac
     1981 attgaaccgg ccgtcgccgc tgtgcgttgc gaagatctcc tcgcggtgct tgtcggcctt
     2041 acccaggagc acgccagcga acgagcgtcc gtcgatctcg gccggaggct tgcctccggc
     2101 caactcgacc agcgtgggca agaggtcgat ccagctgacc atcgcatccg cactcgcgcc
     2161 ggccttaatt cccggcccgg tcacgatcaa cggcacgcga atgccggaat cgtagaggtt
     2221 ccatttgcca aacggccact gcgccccgtg gtcgctggtg aagaagacga aggtgttctt
     2281 tgggtcgagc gattttccga ccgcttccag gacgctgccc aaatcgtcgt cggccttggt
     2341 gaccgcggcg cagtaccgcg agcgccacag ccgagtttcg ggcgtatcga cgaaggtcgg
     2401 cggcaggatg gttttggacg gctcgtagcc gggcgtctcg cgcggccagg gcacgtgcgg
     2461 ccagttggtc ccgaccatca ggcagagcgg cttgtcgctc ttgcgctgct tcagccaatc
     2521 gacggccagg ccgatccccg tgtagttccc gccgccctcg cccgcgacgt agtcgatgcc
     2581 gtaatacgag ccatggttgt agtgcgcgac cttgccgaac gaggcgactt cgtagcccag
     2641 ctcctgcaag tatgcgggga gcttcttgat ttccgcccgc ggccgcgtgt gatttggctc
     2701 cgccccgttc cgcgccggca tcaatcccgt cagcagcgcc gcccggcttg gcgcacacga
     2761 cggcgaagcc acaaacgcct gcgtgaactt cagcccctcg cgcgctagcc gatcgaagat
     2821 cggcgtgcgg acctccttcg agccatacgg cgtcgtatcg agcccgccca ggtcgtcggc
     2881 gatgaaaacg acgaggttgg gttgagactg ggcccagcac ggcaacatcg ccgcgatcac
     2941 aattcccagt ccga
//
LOCUS       DASRJB010000578         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2130915, whole genome shotgun sequence.
ACCESSION   DASRJB010000578 DASRJB010000000
VERSION     DASRJB010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130915"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFB80_15160"
     CDS             complement(<1..848)
                     /locus_tag="VFB80_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35166.1"
                     /translation="MAEPAAALKNGKLADPNFFPLAVWLQSTSNAAKYKAAGINTYVA
                     LWRGPNDEQLAELAKHGMVAICSQNAAGRAHIGDATILAWMHGDEPDNAQAIRGQKGY
                     GPPIEPAKIIADYERIRAADPSRPVLLNLGQGVAWDNYVGRGVRRNHPEDYAEYVQGC
                     DIASFDIYPACHDNQEVAGKLWFVPDGVSRLRKWAGDSRLVWNCVECTRIHNKTTKAT
                     PQQVKSEVWMSLIRGSKGIIYFCHEWEPRFVEAGLLADAEMLQEVTAVNQQIQKLAPV
                     LHSPDVT"
     gene            complement(919..>2734)
                     /locus_tag="VFB80_15165"
     CDS             complement(919..>2734)
                     /locus_tag="VFB80_15165"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZN35167.1"
                     /translation="IMVANWQREVSRARLVAEVGLEQARLLAPTDPLRDFAPAPGLDL
                     SRVPASVLQGYIAATRALKFAPSTTESNNWVVDGSLSASGKPLLASDPHRPIVLPSLR
                     YLVHLHAPGWNVIGSGEPALPGIALGHNEKIAWGFTIVGTDQSDLFVEETNPNDPRQY
                     KVAGRWEPMRIVRDSLKVKGRTEPLSVELRFTRHGPVVHQDESKHIAIALKWIGSEPG
                     GAAYLGALAVARAQNKEQFLQAIDACKVPSENFVYADVDGNIGWVAAAETPIRKGYDG
                     LLPVPGASDNFEWQGFRPVSELPQRFNPPEHWIATANHNILPAGYPHAIAYEWAAPHR
                     FLRVRERLTAQPKFTLADFQSIQHECTSLPGQALGRVLKGVEVSAELETFKRMLTEWD
                     GVLSVDAKAGPLYAAWLQELMKAAYDSRMPPDARTDRGDLRSISTLLAQLEQPSAAWF
                     GADPIRARDEMVRTTLAAAAARVKKLLGDDPSQWRWGKLHTAWLEHPLASLSQAHAEA
                     FNLGPVPRAGDSHTPLNTRHDENFRQLHGASYRQVLDLADWDRGLATSTPGQSGQPGS
                     PHYGDLLPLWAAGEYFPLAYSRARVEEVTLHRLKLMPK"
BASE COUNT          403 a          884 c          944 g          503 t
ORIGIN      
        1 gtgacatccg ggctatggag caccggcgcg agcttctgaa tctgctggtt tacggccgtg
       61 acctcctgga gcatctcggc gtccgccagc aggcccgctt cgacgaaccg cggctcccac
      121 tcgtggcaga aatagatgat ccccttggag ccgcggatca gcgacatcca gacctcggat
      181 ttgacctgct gcggcgtggc cttggtcgtc ttattgtgga ttcgcgtgca ttcgacgcag
      241 ttccagacca gccgcgaatc gcccgcccat ttccgcagcc gcgagacgcc atcggggacg
      301 aaccacagct tgccggcgac ttcctggttg tcgtggcagg ccgggtagat gtcgaacgag
      361 gcgatgtcgc agccctggac gtactcggcg tagtcctcgg ggtgattgcg gcggacgccg
      421 cggccgacgt agttgtccca ggcgacgccc tggcccaggt ttagcagcac cggccgcgaa
      481 ggatcggccg ctcttatccg ctcgtagtcg gcgatgatct tggccggctc gatgggcggg
      541 ccgtagcctt tctggccgcg gatcgcctgg gcgttgtccg gctcatcgcc gtgcatccag
      601 gccaggatcg tcgcgtcgcc gatgtgcgcg cggccagccg cgttctgcga gcagatggcc
      661 accatcccgt gcttcgctag ctcggcgagc tgctcgtcgt tcggcccgcg ccacagggcg
      721 acgtaggtgt tgatgccggc tgccttgtac ttggcggcgt tgcttgtgct ttgcagccag
      781 accgccagcg ggaaaaagtt cgggtcggcc agcttgccgt tcttcagcgc ggcagccggc
      841 tcggccatgg ggatggagag tgccagctac aggagggacg tcatgcgatg gggaattggg
      901 gatttgggat tgtcttgatc actttggcat gagctttagc cggtgcagcg tgacttcctc
      961 gaccctggcc cgcgaatagg ccagcgggaa atactcgccg gcggcccaca gcggcagcag
     1021 gtcgccgtag tgcgggctgc caggctggcc ggattgcccg ggcgtgctgg tggccagacc
     1081 gcggtcccaa tcggccaggt cgaggacttg ccggtacgat gcaccgtgga gctggcgaaa
     1141 attctcgtcg tgacgcgtgt tgagcggcgt gtgcgagtcg cccgccctcg gcactgggcc
     1201 gagattgaac gcttccgcat gcgcctggct caggctcgcc agcggatgtt cgagccaggc
     1261 cgtgtgcagc ttcccccacc gccactggct cggatcgtcg ccgagcagct tcttcacgcg
     1321 ggcggcggcc gcggcgagcg tcgtccggac catctcgtcg cgagctcgga tcgggtcggc
     1381 gccgaaccag gcggccgacg gttgctccaa ttgcgccagc agcgtggaga tgctccgcag
     1441 gtcgcctcga tcggtccgcg cgtcgggcgg catgcggctg tcatacgcgg ctttcatcaa
     1501 ttcctgcagc caggccgcgt acagcggacc ggcctttgca tccaccgaaa ggacgccgtc
     1561 ccattccgtg agcattcgct tgaaggtctc cagctccgcg gaaacttcga cacccttcag
     1621 aacccggccg agcgcctggc cgggcagcga cgtgcactcg tgctggatcg actgaaaatc
     1681 ggccagcgtg aatttcggct gcgccgtcag ccgctcgcgg acccgcagga agcggtgcgg
     1741 tgcggcccat tcgtaggcga tggcgtgcgg atagcccgcg ggcaggatgt tgtggttggc
     1801 cgtggcgatc caatgctccg gcggattgaa ccgctgcggc agctcgctca cggggcgaaa
     1861 gccctgccat tcgaagttgt cagacgctcc gggcacgggc agcaggccgt catagccttt
     1921 gcggatcggc gtctcggccg cggcgaccca gccgatgttg ccgtcgacgt cggcgtagac
     1981 gaagttttcg ctgggcactt tgcaggcatc gatcgcttgc aggaactgct ccttgttctg
     2041 ggcgcgggcc acggccagtg cgcccagata cgccgcgccg cccggctcgc tgccgatcca
     2101 cttcagggcg atcgcgatgt gtttgctctc gtcttgatgc acgaccgggc cgtgccgcgt
     2161 gaatcgcaat tcgacgctca gcggttctgt gcggcctttg accttcagcg aatcgcggac
     2221 gatccgcatc ggctcccagc ggccagcgac cttgtactgc cgcgggtcgt tcggattggt
     2281 ctcttccaca aacaggtcgc tctggtcggt gccgacgatc gtgaagcccc aggcgatctt
     2341 ttcgttgtgg cccagggcga tgccgggcag cgccggctcg ccgctgccga tgacgttcca
     2401 gccgggagcg tgcaggtgga ccagataccg caggctcggc aggacgatcg gccggtgcgg
     2461 atcgctggcc aggagcggct tgccggatgc cgagagcgag ccatcgacga cccagttgtt
     2521 gctctcggtc gtgctgggcg cgaatttgag cgcgcgcgtc gcggcgatat agccttggag
     2581 cacgctcgcg gggacgcgcg aaaggtccaa gcccggcgcg ggggcgaaat cgcgcagcgg
     2641 atcggtcggc gcgagcagcc gggcctgctc gaggccgacc tctgccacca ggcgggcccg
     2701 cgagacttcg cgctgccaat tcgccaccat gatg
//
LOCUS       DASRJB010000579         6056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_165012, whole genome shotgun sequence.
ACCESSION   DASRJB010000579 DASRJB010000000
VERSION     DASRJB010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165012"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFB80_15170"
     CDS             complement(<1..734)
                     /locus_tag="VFB80_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN35168.1"
                     /translation="MSAEFADKSGVDGVLARFRAAMPIASRVAYFDHAAVAPLPESAR
                     DAIRGWLDQATEEGDPIWTQWARRLEEVRQAAAGLINAQPAEIALVPNTTSGIGLVAE
                     GLPWQRGDNAVTLENEFPSNQYPWMNLASRGVEVRRVPAPGGALDLNRLEEACDARTR
                     IVSISWVGYATGWRIDPAEVAAICQRRGCLFFLDAIQGLGVFPLDVRAAGVDFLAADG
                     HKWLLGPEGAGLLYIRQELLPELRPL"
     gene            858..1040
                     /locus_tag="VFB80_15175"
     CDS             858..1040
                     /locus_tag="VFB80_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35169.1"
                     /translation="MPKALCMSGIAVAILIILLFAADLIIGLPFKRASIFMDIGLLIA
                     AVVLGAISWFTLREQA"
     gene            complement(1041..1820)
                     /locus_tag="VFB80_15180"
     CDS             complement(1041..1820)
                     /locus_tag="VFB80_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35170.1"
                     /translation="MGVGRGTGRLILSVDLELDLAHHEPDQRRLDEVRKRLVDFTRTL
                     RLPATWAVADPVLSAATESILAAGVGHEIAVLGERAWLGRGCGRVRLERELARRFENA
                     RRAGIAVSTLALRNVEQVLDLDLLLDHGVTAVRGPAVESRQAARKLAAPPIRYGIWQP
                     PAAWKIPPQSAWWTPSGWLIRREIRRAIRQQTILHLEIDAPRLVSAPDQAMTLVTAVL
                     AYAAARRDAGQLEVLTIGQVARQALAERTSAPSRSILRPAA"
     gene            2003..2614
                     /gene="nadD"
                     /locus_tag="VFB80_15185"
     CDS             2003..2614
                     /gene="nadD"
                     /locus_tag="VFB80_15185"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZN35171.1"
                     /translation="MRLAIYGGSFDPVHYAHLLLAECCREQLRLDEVWLIPAAVPPHK
                     QSRERASGKARLEMLELALAGNEHLRACSLEIDRGGVSYTVETLAAIATEQPQASLFL
                     LLGADSLLDLPSWREPERICNLALPVIVHRGGAAAPDLSVLAPLVAPERLEEIRAAQV
                     QMPLMDLSSTDLRQRVAAGRSLRFRTPRAVEKYIEAQGLYRGE"
     gene            2695..2985
                     /locus_tag="VFB80_15190"
     CDS             2695..2985
                     /locus_tag="VFB80_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35172.1"
                     /translation="MLRYHDFVPQQTKPAVFFSPAEHESFDAALVDANRWIKENEIRV
                     VSVETVVLPNIWSRFEEGSRDPSLGAGDSVNFWHQFIRVWYEVGAIQSGEQG"
     gene            complement(2982..4331)
                     /gene="bioA"
                     /locus_tag="VFB80_15195"
     CDS             complement(2982..4331)
                     /gene="bioA"
                     /locus_tag="VFB80_15195"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZN35173.1"
                     /translation="MVSADELKLWDRRHVWHAFTQMAEYEPLLIERASGCTLVDTGGR
                     EYIDGVSSLWCNVHGHRHPRLDAAIREQLDKVAHVTSLGLGNPTTARLARRLAEIAPR
                     GLTHVFFSDSGATAVEVALKMAFQYWRQCREPQPRRTKYLCFDDAYHGDTIGSVSLGG
                     VSRFSEMFQPLLFEVVRLPSPGMYRLPPGVTRETACGYYLGLLEQSLEEHGQTLAAVV
                     IEPLVQAAAGMVTHPPGFLRGVRELTQRHGVLLIADEVAVGMGKTGRMFACEHEEVVP
                     DLLCIAKGLTGGYLPVAATLANDEIYGAFLGRYEESKTFFHGHTYGGNPLGAAVALAT
                     LEVFAEERTLENLPPKVSRLADHLSRIAKLPHVGDVRQQGMMAGIELVRDRNTKEPYP
                     WAEKRGIRVCQHARSEGVWLRPLGNVVVVMPALAISLAQIDRICGAVERGIAATTAD"
     gene            complement(4325..4612)
                     /locus_tag="VFB80_15200"
     CDS             complement(4325..4612)
                     /locus_tag="VFB80_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HZN35174.1"
                     /translation="MPGQLQGKIVAYSESGNLVSDIAGDQLAGVPRGEEVTVTCDEHQ
                     TVGIYPADHPEEPFTLLAILGPSGCLELTIVGESAKAMLGVRLGEAIVVKW"
     gene            complement(4709..5536)
                     /locus_tag="VFB80_15205"
     CDS             complement(4709..5536)
                     /locus_tag="VFB80_15205"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZN35175.1"
                     /translation="MRPLITLTTDFGPSSPYVAQMKGVILSLCREVDLVDITHAIGPQ
                     NVREGAVVLADATPRFPAGAIHVAVVDPGVGTARRLVYAEIGVQRYLAPDNGLLSFLA
                     SREPPRQIVVLENAQFWLPQPSRTFHGRDILSPVAAHLAGGLDPVELGPPLDQIEMLP
                     WPRPVKSGETISGEVLYVDSFGNLITNLGLDDIAALGTSATVVECRGRSIESVVPTYG
                     AAAEGELIALFDSQGRLEIAIAGASAARTLGVRVGEPVIVRLKTPDTSQNSEDWREI"
     gene            5612..>6056
                     /locus_tag="VFB80_15210"
     CDS             5612..>6056
                     /locus_tag="VFB80_15210"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN35176.1"
                     /translation="MKPALNDASGRMPPAPIPPLEPGDRLTRDEFERRYEAMPHLKKA
                     ELIEGVVYMPSPVRFYRHGTPQSHLVTWLGNYRAATPGCEVGDNTTARLDMDNEPQPD
                     AMLLIEPMCGGQVKVSEDDYIEGAPELVAEVASSSVSFDLHTKFNV"
BASE COUNT         1027 a         2043 c         1968 g         1018 t
ORIGIN      
        1 atcagtggcc gcagctctgg cagcagctcc tgccggatat agagcaggcc agcgccttcc
       61 ggtccgagca gccacttgtg accgtctgcc gccagaaaat cgacgcccgc cgcccgtacg
      121 tcgagtggga acacgcccag cccctggatc gcgtcgagaa agaacaggca gccgcggcgc
      181 tggcaaatcg cggcaacctc cgcggggtca attcgccagc ccgtggcgta gcccacccag
      241 ctgattgaca cgatccgcgt ccgtgcgtcg caggcttcct cgagccggtt caggtcgagg
      301 gcgccgcccg gcgcgggcac tcggcggact tccacgccgc gcgaagcgag gttcatccaa
      361 gggtattggt tcgaggggaa ctcattctcc agagtgacgg cgttatcacc cctttgccag
      421 ggaagcccct cggcgaccag gccaatgccg ctcgtggtgt tggggaccaa agcgatctcc
      481 gccggctgcg cgttgattaa cccggcggcg gcctgccgga cttcctccag ccggcgggcc
      541 cactgagtcc agattggatc gccctcttcg gtcgcctggt caagccagcc gcggatggca
      601 tctcgcgcgc tctcgggtag cggagcgacg gccgcgtggt caaaataggc cactcgcgag
      661 gcgatcggca tcgccgcgcg gaagcgggcg aggacgccgt ccacgccgga tttatcggca
      721 aattcggcag acacggactc gtgggagatc gagccgcagg ccagcggcgg gcgtgacttc
      781 gaatcagccc gaattatact agcactgcaa acatttgtgt tgacatgctt gagttaagca
      841 cctcgaggat gatgggaatg ccaaaggccc tgtgcatgtc ggggatcgcc gttgccatcc
      901 tcatcatcct gctgtttgcc gccgatttga tcatcgggct gccgttcaag cgggcgagca
      961 tcttcatgga cattggcctg ctgattgcgg cggtcgtatt gggcgccatc agctggttca
     1021 cgctgcggga gcaggcgtag tcaggccgcc ggccgcaaga tcgatcggct cggcgcgctg
     1081 gttcgctcgg cgagggcttg cctggcgacc tggccgatcg ttagcacttc gagctggccg
     1141 gcgtctcgcc gggcggcggc ataggccagc acagccgtca ccagcgtcat cgcctgatcc
     1201 ggcgcactga ccaggcgcgg cgcgtcgatc tccagatgga ggatggtctg ctggcgtatg
     1261 gcccggcgga tttcgcggcg gatgagccat ccggagggag tccaccacgc ggactgcggc
     1321 ggaatcttcc aggcggccgg cggctgccag atcccgtacc ggatcggcgg cgcggctagt
     1381 ttgcgggccg cttggcgaga ttcaacggct ggaccgcgaa ccgccgtgac tccgtgatcc
     1441 agcagcaaat cgaggtccaa gacctgctcg acgtttcgca aggcaagggt gctgacggcg
     1501 attccggccc ggcgggcgtt ttcgaaccgg cgcgccagct cccgctccag ccgcacgcgg
     1561 ccgcagcccc tgcccagcca ggcccgctcg cccagcacgg caatctcgtg cccgacgccg
     1621 gcggccagga tcgattccgt ggctgcggac aataccggat cggcgacggc ccacgtcgcc
     1681 ggcaggcgga gcgtccgggt gaaatcgacc agccgcttcc gcacctcatc gagccggcgc
     1741 tgatccggtt cgtggtgcgc caggtccagt tccagatcca ccgaaagaat cagccgcccc
     1801 gtgccgcgtc cgacgcccat atgccgtccc ttgtccctcg cttgacgcct tccctgcaca
     1861 gctctagtca tcgtccaatg gccgcgggtg gataaaactc ccagtcgtct gccgttccaa
     1921 tcgttaccgc agggcgagag ccgcggggtt ataatcagca cggattgatc gtgcttacct
     1981 gccgcggtga actgattact gtatgcgtct ggcaatctac ggcggctcgt tcgacccggt
     2041 gcactacgcc cacttgctcc tggccgagtg ctgccgggag caactgcggc tggacgaggt
     2101 ctggctgatt cccgcggcgg tgccgcccca caagcagtcg cgcgagcggg cctcggggaa
     2161 ggcccggctg gagatgctgg agctggccct ggccggcaac gagcacctgc gggcgtgcag
     2221 cctggagatc gaccgcggcg gcgtcagcta cacggtcgag acgctggcag cgatcgcgac
     2281 ggagcagccg caggcgagct tgtttctgct cctcggcgcc gattcgctgc tggacctgcc
     2341 cagctggcgg gagccggagc ggatctgcaa cctggcgctg cccgtcatcg tccaccgcgg
     2401 cggtgcagcc gcgcccgacc tgtcggtgct cgcgccgctg gttgcgcccg agcggctcga
     2461 ggagatccgc gcggcacagg tccagatgcc gctgatggac ctctccagca ccgacctgcg
     2521 ccagcgcgtc gccgcaggcc gcagcctgcg tttccggacg ccgcgggcgg tggaaaagta
     2581 tattgaggct caagggctgt atcggggtga gtgaataggc agtgagtggt gagtggtgag
     2641 tggtgttaaa ctggaagaat accactcact actcaccact cactcctccc ccccatgctc
     2701 cgctaccacg acttcgtccc gcagcagacc aagccggcgg tgttcttctc gcctgccgag
     2761 cacgaatcgt tcgacgcggc gctggtcgac gcgaaccgct ggatcaagga gaacgaaatc
     2821 cgcgtcgtca gcgtcgagac ggtcgtgctg cccaacatct ggagccggtt cgaagagggc
     2881 tcgcgcgatc cgtcgctggg cgccggcgac agcgtcaact tctggcacca gttcatccgc
     2941 gtctggtacg aagtcggtgc aatccagtcc ggcgagcaag gctaatcggc ggtggtcgcg
     3001 gcgatgccgc gctcgacggc cccgcagatg cggtcgattt gcgcgagcga gatcgccagc
     3061 gccggcatga cgacgaccac gttgcccaag gggcggagcc acacgccttc gctgcgagcg
     3121 tgctggcaga cgcggatgcc gcgcttttcc gcccagggat acggctcttt cgtattgcgg
     3181 tctcggacca gctcgatccc cgccatcatt ccttgttgcc gcacgtcgcc cacgtgcggc
     3241 agtttcgcga tgcgactgag atgatcggcc agccgcgaga ccttcggcgg cagattctcc
     3301 agcgtgcgct cctctgcgaa gacttcgagc gtcgccaggg cgaccgccgc gccgagcgga
     3361 ttgccgccgt aggtgtgtcc gtgaaagaac gtcttcgatt cttcgtagcg gccgaggaac
     3421 gcgccgtaga tctcgtcatt ggccagcgtc gctgcgacgg gcaggtagcc gccggtcagc
     3481 cctttggcaa tgcacaacaa gtccggcacg acttcctcgt gctcgcaggc gaacatccgg
     3541 cccgtcttgc ccatgccgac ggctacttcg tcggcaatca gcagcacgcc gtgccgctgc
     3601 gtcaactcgc gcacgccccg cagaaatccc ggcggatgcg tcaccatgcc cgccgccgcc
     3661 tgcaccagcg gctcgatcac caccgcggcc agcgtctggc cgtgctcctc gagcgattgt
     3721 tccagcaaac ccaggtaata gccgcaggcc gtctcgcgcg tcactccggg cggcaggcga
     3781 tacatcccgg ggctcggcag ccgcaccacc tcgaacagca gtggctgaaa catctccgag
     3841 aaccgtgaga cgccgcccaa actgacgctg ccgatcgtgt ccccgtgata ggcgtcgtcg
     3901 aagcacaggt acttcgttcg ccgcggctgc ggctcgcgac actgccgcca gtactgaaac
     3961 gccatcttga gggcgacctc gaccgccgtc gcgccgctgt cggagaagaa cacgtgcgtc
     4021 agcccccgcg gcgcgatctc cgccagccgc cgggccagcc gggccgtcgt cggattgccc
     4081 aggcccagcg acgtcacatg ggccaccttg tcgagttgct cgcgaatcgc cgcgtcgagc
     4141 cgcggatgcc ggtgcccatg tacgttgcac cacaggctgc tcacgccgtc gatatattcg
     4201 cggccgcccg tatcgacgag cgtgcagccg ctggcccgct cgatgagcag cggctcgtac
     4261 tccgccatct gggtgaacgc gtgccagacg tgcctgcggt cccaaagttt cagctcgtcg
     4321 gcgctcacca tttgacgacg atcgcctcgc ccagccgcac gccgagcatc gccttggcgc
     4381 tttcgccgac gatcgtcagc tccaggcagc cgctgggccc gaggatcgcc agcagcgtga
     4441 acggctcttc gggatgatcg gccggataga tgccgacggt ctggtgctcg tcgcaggtga
     4501 ccgtcacttc ctcgccgcga ggaacgccgg ccagctgatc gccggcgatg tcggaaacga
     4561 gattgcccga ctcgctgtag gcgacgatct ttccttgaag ctggcccggc aaggcggaag
     4621 ctcccgtccg tgtcggagtg aatcgcgagc cgcattgtaa ggattgcctg gggcaaggcc
     4681 aacccttttg acttcggttg aatcgccatc aaatctcgcg ccaatcctcc gaattctggc
     4741 tggtatccgg cgtcttgagt cgcacgatca cgggctctcc aacccgcacg cccagcgtac
     4801 gagcagcgct agcgccagcg atggcgatct ccagtcggcc ttgggagtcg aacagcgcga
     4861 tcagctcgcc ctccgccgct gcgccatagg tgggaacgac actctcgatc gatcggccgc
     4921 ggcactccac gacggtcgcg gatgtcccga gtgcggcaat atcgtccagg ccgaggttcg
     4981 tgatcagatt gccgaacgaa tcgacgtaca gcacttcgcc gctgatcgtc tcgcccgatt
     5041 tcaccggccg cggccagggg agcatttcga tctgatccag cggcggcccg agctcgaccg
     5101 gatcgaggcc gccggccaga tgcgccgcga ccggcgagag gatgtcccgg ccgtggaacg
     5161 tgcgcgaggg ctgcggcagc cagaactggg cgttttccag aacgacgatt tgccgcgggg
     5221 gctcgcggct ggcgagaaag ctcaataggc cgttgtccgg cgcgaggtag cgctgcactc
     5281 caatctcggc atacaccagc cgccgcgccg tgcccacccc gggatcgacg acggccacgt
     5341 ggatcgcccc cgccggaaac cgcggcgtcg catcggccaa aacgactgcc ccttcccgca
     5401 cattctgcgg cccgatcgcg tgcgtaatat cgacgagatc gacctcgcgg cacagcgaga
     5461 ggatgacgcc cttcatctgc gcgacgtagg ggctgctcgg tccgaaatcg gtcgtcagtg
     5521 tgatgagcgg acgcatcgca gcccgcggtc gcaagaactg gcgtttccag ggtagaatag
     5581 agccgtagga cgaatccggg aaggagcagc catgaagccg gccctgaacg atgcgagcgg
     5641 gcgtatgccg cccgcgccga ttccgccgct ggagccggga gatcggctca cgcgcgatga
     5701 gttcgagcgg cgctacgagg cgatgcccca tttgaagaag gcggagttga tcgaaggagt
     5761 cgtctatatg ccctcgccgg ttcgttttta tcgccacggt acgccgcaga gccacctggt
     5821 gacctggctc ggcaattacc gggctgccac cccggggtgc gaggtaggcg acaacaccac
     5881 ggcgcggctc gacatggaca acgaaccgca gccggatgcc atgctgctca tcgaaccgat
     5941 gtgcggcggc caggtgaagg tgagtgaaga cgattacatt gagggagcgc ccgaattggt
     6001 tgccgaggtc gcctccagct ccgtcagctt cgacctgcac accaagttca acgtct
//
LOCUS       DASRJB010000580         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3267857, whole genome shotgun sequence.
ACCESSION   DASRJB010000580 DASRJB010000000
VERSION     DASRJB010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267857"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFB80_15215"
     CDS             complement(<1..603)
                     /locus_tag="VFB80_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN35177.1"
                     /translation="MNRRWPTWGIVAVTISAGWLSAAEPAKDDIEFFEKKIRPVLVEH
                     CYKCHSEKAEKLQGKLLLDSREAARKGGESGAAVVPGDPDGSLLVQALRFENFEMPPG
                     GKLPAEVIADFERWISRGAADPRDGSGLPAAAPITIDFAKGRQFWSFQPPQPHTAPPV
                     ANETWSRRKIDGFLLAPLEQSGLEPASEADRRTWLRRVTFD"
     gene            complement(842..2167)
                     /locus_tag="VFB80_15220"
     CDS             complement(842..2167)
                     /locus_tag="VFB80_15220"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN35178.1"
                     /translation="MCLLLLSLAKAALAEAPSFKGDIAPLLLGNCLACHGPKKAEGGY
                     RIDTYERVIAAGDSTSPGFHAKDLEGSEAFRRITSTDVKERMPFEGDPLTAEQIALLK
                     KWIEEGAPFDGPDPKAPLASYIPPPTHPAAPESYRATMPITAVEFSPDGSQLLVGGYH
                     EVTVWNPADGKLIRRIANVGQRTYAIRFSPDGQLLAVACGAPGKLGEVRLFRPDSGEL
                     VKVLGLTSDVVFDCAFNPAGDRLATAAADGAVRVFEVASGQEQLTITSHSDWVFCTAF
                     SADGSQLATGSRDKTAKVFDAKTGELLITFNGHNQPVRGVLFHPEGKEVYSAGSDNKL
                     QRWNIAEGKKAAEAGFGGEAYKLTAAGEFFLAEAADNKVRQFNAKDQKQIREFTGPKD
                     WVLCTAWHAGTKRLAGGTFDGQVFVWNADDGAVVTSFYAAPGYQPQEGK"
     gene            complement(2329..>3890)
                     /locus_tag="VFB80_15225"
     CDS             complement(2329..>3890)
                     /locus_tag="VFB80_15225"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN35179.1"
                     /translation="IIVRYLEHIESCSLTFVKDVPNYVWNNPPAANYIDELVAAKLRQ
                     LQYQPAGVCSDEEFLRRVYLDVIGQLPTLAETKEFLADTAENKRAKLVDRLLERPEHA
                     RFFALKWGDLLRLTSNQIGNSGVFKYHRWVERAFVTNMPYDKFARELISSSGSTLDNP
                     AANFYRTATDTQDSVESISQIFLGARLQCAKCHNHPFERWTQDNYYGMAAFFNRVQRK
                     KSPRGDELLIYVAASGEVTQPRTGKQMKPWLPGKGEIDPPAGSDRRGPFVEWLTSKDN
                     LLFAKVEANRLWSFVLGRGIVDPPDDFRDSNPPSNAALLDALTRDFIDHGFDRNHLLR
                     TILNSRTYQADFRPGEFNKDETKYFSHYQPRLLSAAQLLDGICAFTDLPEKFAGLPEG
                     TKAIGIPAPDIVKHDFLKIFGQPERTTVCACERAGDSNLGMAIQFFNGPLIYGKLKDA
                     NNRFRKLLAAGKPHDEIITELYLAAVCRQPTAKELEASRAHIAAKGDAAVAFEDIAWA
                     ILNTNEFLFQH"
BASE COUNT          636 a         1238 c         1267 g          749 t
ORIGIN      
        1 gtcaaaggtg acgcgccgca gccaggtccg gcggtcggcc tcgctcgccg gctcgagccc
       61 cgattgttcc agcggcgcga ggaggaaccc gtcgatcttc cgccgcgacc atgtctcgtt
      121 cgccaccggc ggggcagtat gcggctgtgg cggctggaac gaccagaact gccggccttt
      181 ggcgaagtcg atcgtgatcg gcgcggccgc cggcaggccc gagccatcgc gcgggtcggc
      241 cgcgccccgg ctgatccagc gctcgaagtc ggcgatcacc tccgccggga gcttgccgcc
      301 gggaggcatt tcgaaattct cgaaccgcag cgcttgcacc agcaggctgc cgtcgggatc
      361 gcctggtacg accgctgcgc cgctctcgcc gcccttgcgg gccgcctcgc ggctgtcgag
      421 cagcagcttg ccttgcagct tttccgcttt ctccgaatgg cacttatagc agtgctccac
      481 cagcaccggc cggatcttct tctcgaagaa ctcgatatcg tccttggctg gctcggcggc
      541 gctgagccac cctgccgaga tggtgacggc gactatcccc catgttggcc atcgccggtt
      601 catatcgctc gctccgcgtg ccgcgatcca tcaaggtcgt tttatcgttg aacgatctcc
      661 aactaaccgt tctcgatcca ggctttcggc tcctcaggcc catgcatgca tactcgcgac
      721 atttttctgg cgccaatttg cgtcgcaaag ccaatctgcg aaacaggtta cgcccattcg
      781 ccgaaatgca gattatgcaa atctatgcat tttaaggggc agtatgcagt gtttgattcg
      841 gctacttccc ctcctgcggc tggtagccgg gagccgcgta aaatgacgtg acgacagcac
      901 cgtcgtcggc gttccagacg aagacctggc cgtcgaaagt gcctccggcc agccgcttgg
      961 ttccggcgtg ccaggcggtg caaaggaccc aatccttggg gccggtgaac tcgcgaatct
     1021 gcttctggtc cttggcgttg aactggcgga ccttgttgtc ggcggcttcg gccaggaaga
     1081 attcgccggc agccgtcagc ttgtaggcct cgccgccgaa gcccgcctcc gccgccttct
     1141 tgccctcggc gatgttccac cgctgcagct tattgtcgct gcccgccgaa tagacctctt
     1201 tcccttcggg gtgaaacagc acgccccgca ccggctggtt gtggccgttg aacgtgatca
     1261 atagctcgcc cgtcttggcg tcgaagactt tcgctgtctt gtcgcggctg ccggtggcga
     1321 gctgcgagcc gtcggcgctg aaggccgtgc aaaagaccca gtcgctgtgg ctggtgatcg
     1381 tgagctgctc ctggccgctg gccacttcaa acacccgcac cgcgccgtcg gcggccgcgg
     1441 tcgccagccg gtcgcctgcc gggttgaacg cgcagtcgaa taccacgtcg ctcgtcaggc
     1501 cgagcacctt caccagctcg ccgctgtcgg gtctgaacaa tcgcacttcg ccgagcttgc
     1561 cgggcgcgcc gcaggcgaca gccagcagct gcccgtcggg gctgaagcgg atcgcatagg
     1621 tccgctggcc gacgttggcg atccggcgga tcagcttgcc gtcggccggg ttccagacgg
     1681 tgacttcgtg atagccgccg acgagaagct gcgatccgtc ggggctgaac tcgacggcgg
     1741 tgatcggcat cgtggcgcgg taggattcgg gggcagccgg atgtgtcggc ggaggaatgt
     1801 acgaggccag cggcgctttc ggatcgggcc cgtcgaacgg cgcgccctcc tcgatccact
     1861 tcttcaaaag ggcgatctgc tccgcggtca gcgggtcgcc ttcaaagggc atccgctcct
     1921 tgacgtcggt cgaggtgatt cgccgaaaag cctcgctgcc ctccaggtcc ttagcatgga
     1981 acccgggcga ggtcgaatcg cccgcggcga tcacccgttc gtacgtatcg atgcggtagc
     2041 ccccctcggc tttcttcggc ccgtggcagg ccaggcagtt gccgagcagc agcggagcga
     2101 tgtcgccctt gaagctggga gcttcggcca gtgccgcttt cgccagcgag agcagcagca
     2161 gacacacggc cactcgttcg caagctccgc ttgggaacgc aagcaccgaa gctctgcttc
     2221 gagaagtgga agcagagctt ccgagacgtg ggttcccaag cagagcttgg gaaccaggaa
     2281 cgagactgtg catgttctta ctcggcagac aagaatgtct gccccacttt agtgctggaa
     2341 caggaactcg ttggtgttca ggatcgccca ggcgatgtct tcgaacgcga cggcggcatc
     2401 gcctttggcg gcgatgtggg cccggctggc ctccaactcc ttcgcggtcg gctggcggca
     2461 gacggcggcg aggtacagct cggtgatgat ctcgtcgtgc ggcttgccgg cggcgagcag
     2521 cttgcggaag cggttgttcg cgtccttgag cttgccgtag atcagcggcc cgttgaagaa
     2581 ctggatggcc atccccaggt tcgaatcgcc ggcccgctcg caggcacaaa cggtcgtccg
     2641 ctcgggctgc ccgaaaatct taaggaagtc gtgcttcaca atgtccggcg cgggaatgcc
     2701 gatggccttc gtcccctccg gcaggccggc gaacttctcg ggcaggtcgg tgaacgcgca
     2761 aatgccgtcg agcaactgcg cggccgagag cagccgcggc tgatagtgcg aaaaatactt
     2821 cgtctcgtcc ttgttgaatt cgccgggccg gaaatcggcc tgataggtgc ggctattgag
     2881 gatcgtgcgg agcagatggt tgcggtcaaa cccgtggtcg atgaagtcgc gggtcagggc
     2941 gtcgagcagc gcggcgttgc tgggcggatt gctgtcgcgg aaatcatcgg gcggatcgac
     3001 gatgccgcgg cccagcacga agctccacag gcggttggcc tcgaccttgg cgaacaaaag
     3061 gttgtccttg gaagtcagcc actcgacgaa cggcccgcgg cggtcgctgc cggccggcgg
     3121 atcgatctcg cccttgcccg gcagccaggg cttcatctgc ttgccggtcc gcggctgcgt
     3181 gacttcaccg ctggcggcga catagatcag cagctcgtcg ccgcggggcg actttttccg
     3241 ctgcacgcgg ttgaagaagg ccgccatgcc gtaatagttg tcctgcgtcc agcgctcgaa
     3301 cggatgattg tggcacttgg cgcactgcag ccgcgcgccg aggaagatct gcgagatcga
     3361 ttcgacgctg tcctgcgtgt cggtcgctgt gcggtagaaa ttggcagccg gattgtcgag
     3421 cgtgctgcca ctggacgaga tcagctcgcg ggcaaacttg tcgtacggca tgttcgtcac
     3481 aaaggcgcgc tcgacccagc ggtgatactt gaagacgccg ctgttgccga tctgattgct
     3541 cgtcagccgc agcaggtcgc cccacttgag ggcgaagaag cgggcgtgct caggccgctc
     3601 gagcagccga tcgaccagct tcgcccgctt gttctcagcg gtgtccgcca gaaactcctt
     3661 ggtctcggcc agcgtcggca gttggccgat gacgtcgaga tagacgcgcc gcaggaattc
     3721 ctcgtcgctg cacacgccgg cgggctgata ctgcagctgc cgcagcttgg cggcgaccag
     3781 ctcgtcgatg tagttcgccg ccggcggatt gttccagacg tagttcggga cgtccttgac
     3841 gaacgtcagc gagcaggact cgatgtgctc caggtagcgg acgatgatcg
//
LOCUS       DASRJB010000581        15726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_451617, whole genome shotgun sequence.
ACCESSION   DASRJB010000581 DASRJB010000000
VERSION     DASRJB010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451617"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFB80_15230"
     CDS             <1..693
                     /locus_tag="VFB80_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HZN35180.1"
                     /translation="ILFGDYTFVNQLLARHYGLAPADVPADRTVRIADVSGQHRGGIL
                     GMGAVLSVTSAPLRTSAVKRGDWLLRRVLGTPVPPPPADAGSIPAEETLADELTVRQR
                     LEKHRTAASCVSCHSRIDPLGFALEHYDPLGRWRETYRDGKPIDPTGILNDGTKIDGL
                     DSLRQYLRGQQPQFHKNLSSKLLGFALGRTELASDRPLLAQMQADLDEGRGFSALVVH
                     IVTSQQFRNRRP"
     gene            696..2069
                     /locus_tag="VFB80_15235"
     CDS             696..2069
                     /locus_tag="VFB80_15235"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN35181.1"
                     /translation="MAHTHDHRLSRRGFLRGAGGLALALPWLESLPARADDTGKRVAA
                     TAGGPNQPPVRFLCLFFSNGVEPEHWWAKGSGGEMQIGPGLAPMQPHREDIVFLRGLF
                     NEQAVRHKSVHLGRSPNLLSGAWVSTDQGDIRVGRTMDQLLAQRIGGRTAVPSLVLGI
                     EPTELRLEDGLSMLYGSCISWTSETKPATKEIYPSRVYDLLVGGQGRQLDKSILDQVL
                     ADARDLRPKITGADRVKLDEYLESIRDIEQRIDRAADDGRLEGWRPTLAKPDLPRPGE
                     QLPQDFPTHMRLMLDLIVLAFQMDKTRIATCMLNNDLSQMNFGFLEGVQGSLHLDLTH
                     NGREPDREAMYLKTNQFHVSQFAYLLSRLKKIQEGDGTLLDHSLLMCCSNLFDGDRHS
                     ADQMPILLAGKGGGTLQTGRVLDYQGRGDANRRACSLYLSLLDRLGVRLERFGDADQR
                     LADLGDV"
     gene            2799..4694
                     /locus_tag="VFB80_15240"
     CDS             2799..4694
                     /locus_tag="VFB80_15240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35182.1"
                     /translation="MIHRQETVGWAELPGQSPAELPTVRGYEVLAELGRGGMGVVYRA
                     RQASSQRLVALKLIRDGALAGPQDRARFWIEAEAAARMQHPHIVQIYEVAEHGGLPYF
                     AMELVEGGSLEKLLPQGSLESRLAAGLVHKLAQAIEHAHAQNIVHRDLKPANILVAAD
                     QWPGTSSQEPVDSGSWPLAPGSWLLAPGCLKITDFGLAKRLDGESTAWTQPGVILGTA
                     SYMAPEQAAGRVGAIGPATDIYALGAILYELLTGRPPFQAETWNQTLLQVLHDEPQPP
                     TRLNSAVPRDLEAICLKCLEKEPERRYSSAGDLAADLNRFLTSQPVAAVPLSDGERLA
                     RRALQDGYTLVERIGTGPRSTVWRALYGSPQQTVALKVYDAGICTEDQWRDRLNRGAD
                     LWSALAHPQLAGVQRAGWWDGAAYVSMDYVPQGSLADRLAGQDRPGPLKQALQLVAQL
                     AEIVGYLHRQGVVHGNLKSSNVLLAADGIARLSDLRLTGGLFQGSAPAGDDDPAAVAC
                     LAPELLENPHAEVRPYTDIYGLGIVLYELLTGRLPFAGTTAGEVRERIDAGEFAPPSA
                     LNPDVTPRVESVCMRCLKTNPWRRYHRVYDLQIRLQYLSDNPGGPLEPREWWLNRRPP
                     QRGPHPR"
     gene            4704..5588
                     /locus_tag="VFB80_15245"
     CDS             4704..5588
                     /locus_tag="VFB80_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35183.1"
                     /translation="MIHRVLPIALALLPLPVALSLAGAQETPMSQLRQAVTFYASFDE
                     KVAGDFGGGELSPGTRMNHETEAGKFVFQPGVDEKVFTIARGKGIHGGALSPTDVLPR
                     NGRIYFPAKGNIGFKPGGWGGAASFWLNTDPNTLLKTRFCDPLQITEKGANNGGLWFD
                     FNDASPRDARMGAFPAVPAGGQPIKESDPGAPLVPVKAVGFKVGQWHHVLLNWRNFDT
                     AKADAEADLYIDGRRIGGVRDRAIAMNWNLDQAGIYVAVGYIGLLDELAVFGRPLSAA
                     EIEQLFRQPGSLSSLNRK"
     gene            complement(5821..6174)
                     /locus_tag="VFB80_15250"
     CDS             complement(5821..6174)
                     /locus_tag="VFB80_15250"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HZN35184.1"
                     /translation="MRFIFGIAVLMASFQAEAPLAESQKIEALIRHVEQLKDAKFVRN
                     GVEYDAPAAGQFLRGKWKTQEAEIKTAKDFIDKVASVSSTSGKPYLIQLADGREVKSG
                     EYLAGELKKLESAGK"
     gene            6381..6512
                     /locus_tag="VFB80_15255"
     CDS             6381..6512
                     /locus_tag="VFB80_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35185.1"
                     /translation="MSHLRRQPQQLADRQAVSATMLVALAVGVAAAALVMAAISQMI"
     gene            complement(6509..6703)
                     /locus_tag="VFB80_15260"
     CDS             complement(6509..6703)
                     /locus_tag="VFB80_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35186.1"
                     /translation="MQLPETRFVRFVCWLLLVSGAATGGFFAGYRVGYLDGNARFEST
                     YNGVRVRMVPIGEPAANAPR"
     gene            complement(6771..6935)
                     /locus_tag="VFB80_15265"
     CDS             complement(6771..6935)
                     /locus_tag="VFB80_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35187.1"
                     /translation="MQIWRIPAPRAQHGFETAEPWWTHFEAAIWIGLVLLLLLTACLL
                     VACVLSPKKA"
     gene            complement(7040..8005)
                     /locus_tag="VFB80_15270"
     CDS             complement(7040..8005)
                     /locus_tag="VFB80_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZN35188.1"
                     /translation="MLIRIPIVVALLFAAAVVRAQDQPGREADARPLHRIAARTPEEL
                     QGIFKYSGQTLPLVSAHRGGPRSGFPENCLATFEHTLQHTFAMLEIDPRYAKDGAIVV
                     HHDAMLDRTTTGQGLVADKTLAELKQLRLRDADGKPTEFQIPTLDEALQWARGKTILV
                     LDQKDVPVAARVQKVEEHKAEAYAMLIVYSYKEAQACYALNPNVMLEVMVPTREKVAE
                     FDATGVPWRNVVAFVGHTPPTDAALYQAIHERGARCLMGTSRNLDRRFIRGEVTDIKQ
                     LEQDYRGLLRRGADLIETDIPAPLGTLLYGSTGPPAAQRSFFEVK"
     gene            complement(8011..8853)
                     /locus_tag="VFB80_15275"
     CDS             complement(8011..8853)
                     /locus_tag="VFB80_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN35189.1"
                     /translation="MSYPRAIALTSLTMLAFAGNSLLCRLALKQTSIDAASFTTIRLI
                     SGALILGLAARMTRSPVAGSGDWLSAAALFVYAAAFSFAYVSLPAATGALLLFGAVQA
                     TMIGRGFWAGERLVGWQIAGFLIAVGGLVGLLLPGLSAPPLVGALLMLSAGAAWGVYS
                     LRGKGAGDPLRKTAGNFLRAVPLAAALSLIAAGSASLDQAGFWYAAASGALASGIGYA
                     IWYTVLPSLQATTAATVQLSVPVIASLGGIVLLGEPLTLRLVLASLAILGGIALVILD
                     KQRA"
     gene            8981..9229
                     /locus_tag="VFB80_15280"
     CDS             8981..9229
                     /locus_tag="VFB80_15280"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HZN35190.1"
                     /translation="MNWYSACGTIAQNSGGCDVNVGERERWASLIGGGLLAAYGLLRG
                     SALRLPLVLLGGALAYRGYTGHCQVYEALGRNTASGMA"
     gene            complement(9231..10190)
                     /locus_tag="VFB80_15285"
     CDS             complement(9231..10190)
                     /locus_tag="VFB80_15285"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35191.1"
                     /translation="MSQPIGLCLIGAGAIAERHMQALERLGGVLPRWVVSRPAEAAGD
                     FARRWKFAHVGSEVGPALADASVQLAMIASPSPLHSEQAVQALQAGKDAIVEIPAAMS
                     WPDAQNLARVAAAVGRRAWICHTLRSTPALRLVRERIRSSRLRITHIAGFFGIPRRRN
                     QGMGGIGTRSWIDNLLWHHGCHQVDAALWVLGLPAVTSVQALLGPAHPTLGMALDVGV
                     QLTTAGGELITQSLSYNVEQGVWRLQFIGHEDVLTFQDGRLTNEAGQELVPESPLADL
                     TVQDRELLHAFRSGEKSEYDLPSVLATMEVLGRAQESADAAAR"
     gene            complement(10203..10466)
                     /locus_tag="VFB80_15290"
     CDS             complement(10203..10466)
                     /locus_tag="VFB80_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35192.1"
                     /translation="MLVLLVAAAAMRLVQQRAAAGRFQERLADERMLRQAAEVKLAQA
                     AAAKARLQAAQAEKSAVVQSRQAGPATGTDQTSPRSASGPRDP"
     gene            complement(10629..10907)
                     /locus_tag="VFB80_15295"
     CDS             complement(10629..10907)
                     /locus_tag="VFB80_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35193.1"
                     /translation="MRYKLRTLLILLAILPPLLWIGWTKYSEWKAEQERQRAQRELVQ
                     QLDGLQIYYGNPPPRITPPATPPAPTPTDAAAAPDSPPEKAPAVPQRE"
     gene            11284..11356
                     /locus_tag="VFB80_15300"
     tRNA            11284..11356
                     /locus_tag="VFB80_15300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11317..11319,aa:Arg,seq:cct)
     gene            11372..12052
                     /locus_tag="VFB80_15305"
     CDS             11372..12052
                     /locus_tag="VFB80_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HZN35194.1"
                     /translation="MSLFMFSIALIAAPGEDQSVRLNHSLREYVAARAAEFGEIPEER
                     KEQLSKIADYVRGRIKAGQPARLVFICTHNSRRSHLSQIWAQTAASHYGAANVETFSG
                     GTEATAFNPRAVNALKRAGFMISEPPAGENPRYQVRFHDDAPPMECFSKVYSESPNPT
                     ADFCAVMTCSQADQNCPQVRGAALRIALPFDDPKAFDGTPEEAAKYDERCRQIAREML
                     FVFASGLK"
     gene            12075..13445
                     /locus_tag="VFB80_15310"
     CDS             12075..13445
                     /locus_tag="VFB80_15310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN35195.1"
                     /translation="MPEPQGPARETAALRRSRRLPDRADVVVVGAGAAGVFALRELVR
                     GGLTAIGLEARNRIGGRIFTRRTLARHPLELGAEFVHGPNNIVHQLAAEYGLTLVPHA
                     GTAYSWWDGQLVPDDRLPRPPLAVLQEIRGLAERRRRSGGGAMPLAEFLGTPELSEIF
                     ERSAVTRRYVDQLIKNDHSVEPTSLTLEGWLEPDVTGFEANYRIDEGYSALLHRAAEA
                     DDLDIRLNRPVKRIEWEPGRVTCYASGAAVRSRAAIVTLPLGVLQQGDVEFDEPLPRS
                     KVEALESLLPGKAFKMHTSFHALRGGRPFWPAGLAFLTSALDSQLHWPASQERRRGQR
                     HLLTHLVGGDAADRFGQHPDPPQAMLNQLVHMFGNSRIRELFVRAEWRAWHTDPYSRS
                     GYSAFPSDADLGARQALGQSVMDTLFFAGEAVGVKGRPGNVASVHGAIESGIQCARDA
                     IASLRT"
     gene            complement(13468..15318)
                     /locus_tag="VFB80_15315"
     CDS             complement(13468..15318)
                     /locus_tag="VFB80_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN35196.1"
                     /translation="MSERRRVLLIQLPIPQPGMSPAEENVPLAAGYLKLLARLRGLES
                     SYDIEILPARLANQLSDQGLAAEIQGREAWLVGWTCYLWNVERALDVSRLVKQARPEV
                     RILVGGPEITADNGWVLARPEVDFAAIGEGEQTFCELLAELLTGDIPARPIPGLYASP
                     ALARTVRGDWSAEPATRGELPPFRRPLSKLDEISSPYLAGILDAADERVLLLETLRGC
                     VFKCKFCYYPKSYDDLYFASREKLVANLEHARRRGAREVVLLDPTLNQRPDFEGFLKL
                     LAECNPDRQFTYFGELRAEGITANIARHLRAANFTRVEIGLQSTDPLAQQLMDRKNNL
                     QAFERGVRAMQDAGISVKVDLIIGLPGDTPDSIRRSLSYLRENRLSDEVQVFNLAVLP
                     GTAFRQEARELGLEYQSRPPYYVVQTPTLDLADLSLLMEEAQELFETEFDPLPEPAIP
                     DVERAVALGAPASVARIDLDGESWSAAGCTAAEGGLPRPPERTQAFTLWLRAADFRQH
                     GAAARQLVEQLLGENPHTTLQVILEPGDAASVTAALLAELEQACYRRTTYLDRFYSIL
                     PGPLKGSKRLLVLLDPAEAARMDRQAIDSIEEFAEIVCPSPVTPDARCVG"
BASE COUNT         2728 a         5207 c         5125 g         2666 t
ORIGIN      
        1 atcctgtttg gcgattacac attcgtgaac cagttgctgg cccggcacta cgggctggcg
       61 cctgccgatg ttcccgccga tcgcaccgtt cgcatcgccg atgtcagcgg tcagcaccgc
      121 ggcggcatcc tgggcatggg cgcggtgctg agcgtcacct ccgcgccgct gcggaccagc
      181 gccgtcaagc gcggcgactg gctgctccgc cgcgtgctgg gcacgcccgt tccgccgccg
      241 cctgccgacg ccggctcgat tcccgccgag gaaacgctcg ccgacgaatt gaccgttcgc
      301 cagcggctgg agaagcatcg caccgcggcc tcgtgcgtca gttgccactc gcggatcgac
      361 ccgctgggat tcgcgctgga acactacgac ccgctcggcc gctggcgcga gacctatcgc
      421 gacggcaaac cgatcgatcc caccggcatc ctgaatgacg gcacgaaaat cgacggcctg
      481 gacagcctgc ggcagtatct ccgcggccag cagccgcaat ttcataagaa cctgagcagc
      541 aagctgctcg ggtttgccct cggccggacc gagctggcct ccgatcgccc gctgctcgcg
      601 cagatgcagg ccgatctcga cgagggccgc ggcttttcgg cgctcgtcgt tcacatcgtt
      661 accagccagc aatttcgcaa ccgcaggccg tagccatggc gcacactcac gatcaccggt
      721 tgtcgcgccg cggattcctg cgcggggcag gcggcctcgc gctggcgctg ccctggctgg
      781 agtcgctgcc ggctcgggct gacgacacgg gcaaacgcgt ggcggcaacg gccggcgggc
      841 cgaaccagcc gcccgtgcga ttcttgtgtc tcttcttctc caacggcgtc gaaccggagc
      901 actggtgggc caagggcagc ggaggcgaga tgcaaatcgg gccgggcctg gcgccgatgc
      961 agccgcaccg ggaagacatc gtctttctgc ggggcctctt taacgagcag gccgtgcggc
     1021 acaagagcgt ccatttgggc cgcagcccca atctgctttc cggagcctgg gtcagcacgg
     1081 accagggaga catccgcgtc ggccgcacca tggaccagct gctggcccag cggatcggcg
     1141 gccgcaccgc cgtgcccagc ctcgtgctcg gcatcgaacc gaccgaattg cgcctggaag
     1201 acggtctgtc gatgctctac ggctcgtgca tctcgtggac ctcggaaacg aagccggcca
     1261 cgaaggagat ctatccctcg cgggtctatg acttgctcgt cggcggtcag ggccggcagc
     1321 tcgacaagag catcctcgac caggtgctgg ccgacgcccg cgatctgcgg ccgaaaatca
     1381 cgggcgccga ccgcgtcaag ctcgacgaat acctcgaatc gatccgcgac atcgagcagc
     1441 ggatcgaccg cgctgccgac gacggccgcc tggaaggctg gcggcccacg ctggccaaac
     1501 ccgacctgcc gcggcccggc gagcaattgc cgcaggattt ccccacgcac atgcggctga
     1561 tgctcgacct gatcgtgctg gcgttccaga tggacaagac ccgcatcgcg acctgcatgc
     1621 tcaacaacga cctgtcgcag atgaacttcg gcttcctgga gggcgtgcag ggcagcctgc
     1681 atttggacct gacgcacaac ggccgcgagc ccgaccgcga ggcgatgtac ctgaagacga
     1741 accagttcca cgtcagccag ttcgcgtatt tgctgtcgcg gctgaagaag attcaggagg
     1801 gcgacggcac gctgctcgac cattcgctcc tgatgtgctg ctcgaacctg tttgacggcg
     1861 accgccactc ggccgaccag atgccgatcc tgctcgccgg caaaggcggt ggcacgctgc
     1921 aaaccggccg cgtgctcgac tatcagggtc gcggcgacgc caatcgccgc gcgtgcagcc
     1981 tgtacctctc gctgctggac cgcctgggcg tgcggctgga gcggtttggc gacgccgatc
     2041 agcggctggc cgatctggga gacgtctaga cgggacggtc atccccccat ttggaattgg
     2101 ctggaattgg ccctcattgc agagaaaacc gcaaatgcca gtcggtttct agagaaagcc
     2161 gtcgccgccg gccattttct gaagaattgt tgcaagccaa agtggagacg ggacttgtga
     2221 ccggtgctgg cggccgaaaa tgggccccag gcagccgaga gccgcccctt gcgtgtataa
     2281 tgcatcgcat tgtgtactga tgattcaata cgccctgacg ccggacagct tgctgcgatc
     2341 acgccgtgcc gagccgaagc aggagcgcga aagcgcaaaa cgaggaccct cgcgtgacga
     2401 gggccaggtc cctacgaggt ggagctggcg atcgcggatg gccggcggtc gggagcgacg
     2461 gagcccgcgt ggttgctgca gcgacttggc gattgcctaa accgcgcccg tgatccagcg
     2521 cggctggaag cgcagcggcc gttcatccgc cgcaagcggc cggcgacggg tataacagaa
     2581 actctccact aagtcttcaa acccttcaaa ccctatcctg gccgcaatgt atacaaataa
     2641 acataaagtg gttgtcgagc ggcagttgtg acgtgcgagc gactccacgg ggggccgcca
     2701 aaaagtctgc ggagaggagt tttggttata ttttctggcg agagccaacg gccgacttct
     2761 cgccttacac taattgcgat gcgcgagcga gggacgcgat gatccatcgc caagagaccg
     2821 tcggctgggc tgagctgccg gggcaatcgc cggcggagct gccgacggtt cgcggctacg
     2881 aagtcctggc ggagctgggc cgcggaggaa tgggcgtcgt ctatcgggct cggcaggcga
     2941 gctcgcagcg gctggtggcg ctcaagttga tccgcgacgg cgcgctggcc ggaccgcagg
     3001 accgggcccg gttctggatc gaggccgaag cagcggcccg gatgcagcac ccgcacatcg
     3061 tgcagatcta cgaagtcgca gagcacggcg gcctgcccta tttcgcgatg gagctcgtag
     3121 agggcggcag cctggagaag ctgctgccgc aaggatcgct ggagagccgg ctcgcggccg
     3181 ggctggtcca caagctcgcg caagcgatcg agcacgcgca tgcgcagaac atcgtccacc
     3241 gcgatttgaa gccggcgaac atcctcgttg cagcggacca gtggccagga accagtagcc
     3301 aggagccagt ggactccggc tcctggccac tggctcctgg ttcctggctc ctggcccctg
     3361 gctgcttgaa aatcacggac ttcggccttg ccaaacggct cgacggtgag agcaccgcct
     3421 ggacacagcc cggagtgatc ctcggcacgg ccagctacat ggctcccgag caggcggccg
     3481 gccgcgtcgg cgcgattggc ccggcgaccg acatctatgc cctcggcgcg attctgtacg
     3541 agctgctcac cgggcggccg ccctttcagg ccgaaacctg gaaccagacg ctgctgcaag
     3601 tgctgcacga cgagccgcag ccgccgacga ggctcaactc cgccgtcccg cgcgacctgg
     3661 aggcgatctg cctcaagtgc ctggagaagg aaccggagcg gcggtattcc agcgccggcg
     3721 acctggccgc cgatttgaat cgctttctga cttctcagcc cgtggccgct gtgcccttga
     3781 gcgacggtga gcgcctggcc cgccgtgccc tgcaagacgg ctacacgctg gtggagagaa
     3841 tcggcaccgg accgcgaagc accgtctggc gggccctgta cggttcgccg cagcagaccg
     3901 tcgcgctcaa ggtctatgac gccgggatct gcaccgagga tcaatggcgg gaccggctga
     3961 atcgcggcgc ggatctctgg tcggcactcg cgcacccgca gctcgctggg gtccagcgtg
     4021 ccgggtggtg ggacggcgcg gcctatgttt ccatggatta cgtcccgcag ggcagcctgg
     4081 ccgatcggct ggccggtcag gaccggcccg gcccgctcaa acaggcgctg caactggtcg
     4141 cgcagttggc ggaaatcgtc ggctaccttc atcggcaagg agtggtccac gggaacctga
     4201 aatccagcaa cgtacttctc gccgccgacg ggatcgcccg gctcagcgac ctgcggctca
     4261 ccggcgggtt gtttcaaggc tctgctcccg cgggcgacga cgatccggcc gcggtggcat
     4321 gcctggcgcc ggagctgctg gaaaacccgc atgccgaggt gcgcccttat accgatatct
     4381 acggcctggg tatcgttctg tacgagctgc taaccgggcg gctgccgttt gctggaacaa
     4441 ccgcaggcga agtgcgcgag cggatcgacg cgggcgagtt cgccccgcca tccgccctga
     4501 atccggacgt gacacctcgg gtcgagtcgg tttgcatgcg ctgcctgaag accaatcctt
     4561 ggcggcggta ccaccgcgtt tatgacctgc agattcgcct gcagtatttg agcgacaacc
     4621 cgggcggtcc cctcgagcct cgcgagtggt ggctcaatcg tcgcccaccg caacgaggcc
     4681 ctcacccacg ctaggacatc tgtatgattc atcgcgtttt gccgatcgcc cttgccctgt
     4741 tgccattgcc ggtggcgctc agcttggccg gcgctcagga gacgcccatg agccagcttc
     4801 gccaggcggt gacgttctac gcctcgttcg acgagaaggt cgccggcgac tttggcggcg
     4861 gggaactgtc gcccgggacg cgcatgaacc acgagaccga ggccggcaag ttcgtctttc
     4921 agcccggcgt ggatgagaag gtctttacga ttgctcgcgg caagggaatt cacggcggag
     4981 cgctctcgcc gacggatgtg ctgccccgca atggacgcat ctactttccg gccaagggca
     5041 acatcggctt caaacccggc ggctggggcg gcgcggcttc attctggctg aataccgatc
     5101 ccaacacgct gctgaagacc cgcttctgcg acccgctgca aatcaccgag aagggagcaa
     5161 acaacggcgg cctgtggttc gacttcaacg acgcttcgcc ccgcgacgcg cggatggggg
     5221 ctttccccgc ggtgccagcg ggcgggcagc cgatcaagga gagcgatccc ggcgcgccgc
     5281 tcgttcccgt gaaggcggtc ggcttcaagg tgggccagtg gcatcacgtc ctcctgaact
     5341 ggcggaattt cgacactgcc aaggccgacg ccgaggccga cctttacatc gacggccggc
     5401 ggatcggagg cgtgcgtgac cgggccatcg ccatgaattg gaacctggac caggccggca
     5461 tctacgttgc cgtcggctac atcggcctct tggacgagct ggccgtcttc ggccggccgc
     5521 tctcggccgc ggagatcgag caactgttcc ggcagccggg gtcgctgtcg tcactgaatc
     5581 gcaagtagcc tgctcattcc tcatcctatt ggagagggtt agggtgaggg cgcgctgcga
     5641 cttcagatcc tcaccccggc tttcttccaa agagagaggg agcggaggtc agggtagcgc
     5701 atgttcggcc tactgtaggt cccgcctgcc gggcggaacc tgggcggcgc ggtgcgtcca
     5761 attctcggga gcgctccagg tcgctgcgct tccctgaagg ccccttccgg cagaaggggc
     5821 ctacttgccg gcgctttcga gcttcttgag ctcgccggcc aggtactcgc cgctcttcac
     5881 ctcgcggccg tcggccaact ggatgaggta cggcttgccg ctggtggagg agacactggc
     5941 gaccttgtcg atgaagtcct tggcggtctt gatctcggcc tcctgcgtct tccatttgcc
     6001 gcggaggaac tggccggcgg ccggcgcatc gtattcgacg ccgttgcgga cgaacttggc
     6061 atcctttagt tgctcgacgt ggcgaatgag cgcctcgatt ttttgcgatt ccgctagcgg
     6121 cgcttctgcc tggaatgacg ccattagcac ggcgatgcca aagataaatc gcataatgcg
     6181 ggcccttttt acgccctcga ttctatccca gcccaaaaat cctccgccag tgtggcactt
     6241 ccaccgtggc ctccgggaat taatgagaga cgaggatggc cggaacggag attgacgcta
     6301 gcccccaagc gatcgcccgg tggtgctgcg attcgtcgct caccatccgg gcgtcttttt
     6361 tcgaataaca gctatccgcc atgagccatc ttcgccgcca gccccagcag cttgccgatc
     6421 gccaggcggt cagcgcgacg atgctggttg cgctggccgt gggtgtggcg gcggcggcgc
     6481 tggtcatggc ggccatcagc cagatgatct agcggggtgc gttggcggcg ggttcgccga
     6541 tcggcaccat ccggacacgc acgccgttat aggtcgattc gaatcgcgcg ttgccgtcga
     6601 gatagccgac gcggtagccg gcgaagaatc cgccggtcgc cgcgccgctc accaacagca
     6661 gccagcaaac aaagcggacg aagcgagtct caggcagttg cacgatcacc tcagttccaa
     6721 gaacgtggca gcgctacgcc agaatgcagg ctgggccgcg gctgaacaga tcacgccttt
     6781 ttgggcgaga ggacgcaggc caccagcaag caggcagtca gcagcaacag caggaccagg
     6841 ccgatccaaa tcgccgcttc gaagtgggtc caccacggct cagccgtctc gaacccgtgt
     6901 tgggcgcgcg gggcgggaat cctccagatt tgcattcgtg atccacaagc cccaaggagc
     6961 gagcagagtg cttctctagg taatagcgcc tttcttcgca attccccgtc acaaaattcc
     7021 attttgtgcg gaaattcctc tatttcactt caaagaaact ccgctgggcc gctggcggac
     7081 ctgtggatcc gtagagcagg gttcccagcg gagcgggaat gtccgtttcg atcaggtccg
     7141 caccgcgccg cagcagcccc cgatagtcct gctctagctg cttgatgtcg gtcacctcgc
     7201 cgcggatgaa cctccggtcg aggttgcggg aagtgcccat caggcacctg gccccgcgct
     7261 catgaatcgc ctgatacaga gcggcgtccg tcggcggcgt atgccccacg aaggccacga
     7321 cattccgcca gggaacgccc gtggcatcaa actcggcgac tttctcgcgg gtgggaacca
     7381 tcacttccag catgacgttc gggttgagcg catagcaggc ctgggcctcc ttgtagctgt
     7441 agacgatcag catcgcgtag gcctcggcct tgtgctcctc gaccttctgg acgcgggcgg
     7501 cgacggggac gtccttctga tcgagaacca ggatcgtctt gccgcgggcc cactgcagcg
     7561 cttcgtcgag cgtcggaatc tggaactccg tcggctttcc atcggcgtcg cgcaggcgga
     7621 gctgcttcag ttccgccagc gtcttgtccg cgacgagacc ctggcccgtc gtggtacggt
     7681 ccagcatcgc gtcgtggtgc acgacgatcg cgccgtcctt ggcgtaccgc gggtcgattt
     7741 cgagcatcgc aaacgtgtgc tggagagtgt gctcgaacgt cgccaggcaa ttctccggaa
     7801 agccgctccg cgggccgccg cgatgcgcgc tgaccagcgg cagcgtctgg ccgctgtact
     7861 tgaaaatgcc ttgcagctct tccggcgtcc gggcggcaat gcggtgcagc ggccgcgcgt
     7921 cggcttcacg gccaggctgg tcttgcgcac ggacaacggc cgccgcgaaa agcagtgcga
     7981 ccacgatcgg gatgcggatc aacatggtcg tcaggccctt tgtttgtcga gaatcacgag
     8041 cgcgatcccg cccaggatgg ccagcgaagc caggaccagg cgcagggtca gcggctcgcc
     8101 gagcagcacg atgccgccca acgacgcgat gacgggaacg ctgagctgca cggttgcggc
     8161 ggtggtcgcc tgaagcgacg gcagtacggt gtaccagatg gcgtagccga ttccagaagc
     8221 cagcgcgccc gacgcggccg cgtaccagaa gccggcctga tccagcgagg cgctgccggc
     8281 cgcgattagg ctcagtgcag ccgccagtgg aacggctcgc aggaaattgc cggccgtttt
     8341 ccggagagga tcgcccgccc cctttccgcg cagcgaatag acgccccagg ctgcgccggc
     8401 gctcagcatc agcaaagcgc ccaccagcgg cggcgcggag agccccggca gcaacaggcc
     8461 gacgagccct ccaaccgcga tcaggaagcc ggcgatttgc cagcccacca gccgttcgcc
     8521 cgcccagaag ccgcgtccga tcatcgtcgc ctggacagcg ccaaacagca gcaaggcccc
     8581 ggtggcggcg ggcaagctga cgtaggcaaa cgaaaaggcg gccgcataca caaacagggc
     8641 cgccgcggaa agccaatcgc cgctgccggc aactgggctg cgagtcatgc gggcagccag
     8701 acccagaatc aaggcgcccg agattaaacg gatggtcgtg aagctggcgg cgtcgatgct
     8761 ggtctgcttc agggccagcc ggcagagcag cgaattgccc gcaaaggcca gcatggtgag
     8821 cgaggtcagg gcaatcgcgc ggggatacga catggaggac gagcttggag ctgcggtggt
     8881 gcgacaattc tgaccagaag cccgcactgg cacaaacttt gcgagcattt tcccagtccg
     8941 ttggatcaac gattgctatc ttcttcagga ggagccggtc atgaattggt actctgcttg
     9001 cggaacgatc gcccagaatt ctggcggctg cgacgtcaac gtcggcgagc gcgagcgctg
     9061 ggcctcgctg attggcggtg gcttgctcgc ggcatacggc ttgctgcgcg ggtcggccct
     9121 ccggctgccg ctcgtgctgc tcggcggcgc tttggcttat cgcggttaca ccgggcactg
     9181 ccaggtgtac gaagccctcg gccgcaacac cgcgagtgga atggcatagt ttagcgagcc
     9241 gcggcatcgg cactttcctg ggcccggccg agcacttcca tcgtggccag cacgctcggc
     9301 aggtcgtact cggacttttc gccgctgcga aaggcgtgca gcagctcgcg gtcctgaacc
     9361 gtcagatccg ccaagggcga ttccgggacg agttcctgcc ccgcttcgtt cgtcaggcgg
     9421 ccgtcctgga aagtcagcac atcctcgtgc ccgatgaact gcagccgcca aacgccttgc
     9481 tcgacgttgt agctcagcga ctgcgtgatc agctcgccgc cggcggtcgt cagctgcact
     9541 cccacgtcga gcgccattcc cagcgtcgga tgggcaggac ccagcagggc ctgcacgctc
     9601 gtcacggcgg gcaggccgag cacccacagc gcggcgtcca cctggtggca gccgtgatgc
     9661 cacaacaggt tgtcgatcca gctgcgcgtg ccgatgccgc ccatgccctg gttgcgacgc
     9721 cgcggaatgc cgaagaagcc ggcgatatgc gtgatgcgca gccggctgct gcgaatccgc
     9781 tcgcggacca ggcgcagcgc cggcgtggag cggagcgtgt gacagatcca ggcccgccgg
     9841 ccgaccgcgg ccgcgacgcg cgcgaggttc tgcgcgtccg gccagctcat ggccgcggga
     9901 atctcgacga tcgcgtcttt gccggcttgc agcgcctgca cggcttgttc gctgtgcagc
     9961 ggactggggg acgcgatcat cgccagctgc acgcttgcat ccgcgagcgc cggccccacc
    10021 tcggacccaa cgtgcgcgaa tttccagcgg cgggcaaagt cgccggcggc ctcagccggg
    10081 cgcgacacta cccatctggg caaaactccg cccagccgct ccaaggcctg catatgccgc
    10141 tcggcgatgg cccctgcgcc gatcaggcaa agtccaatgg gctgcgacat ggcggcgctg
    10201 catcaaggat cgcgagggcc actggctgag cgcgggctag tctgatccgt gccggtcgct
    10261 ggccccgcct gtcgggactg cacgaccgcg ctcttttccg cctgcgcggc ctgcaggcgc
    10321 gccttggctg cggctgcctg ggccagcttc acctccgctg cctggcgcag catgcgctcg
    10381 tcggccagtc gctcttggaa ccgccctgca gcggcccgtt gctgaaccag ccgcatggcg
    10441 gcagcggcca ccagcagcac gagcaaagct atcgctccga tccaacacgc catcaacatg
    10501 agctcggtag cgctgagcct gtacttccgg ttctgggtcg ccacgtctgc catgccccta
    10561 ttctagccgc gcacgaaaac gccccgccag cgcgaacgga cggggcgggg gcggtgctac
    10621 gtcgccagct actctcgctg cggcactgcc ggcgcttttt ccggcggcga gtccggtgca
    10681 gccgcagcat cggtcggcgt tggagctggc ggcgttgccg gcggcgtgat ccggggcggc
    10741 ggattcccgt aatagatctg caaaccatcc agctgctgca ccagctcgcg ctgcgcccgc
    10801 tgtcgctcct gctccgcctt ccactccgag tacttcgtcc agccgatcca caacagtggc
    10861 ggcaggatgg ccagcaggat caggagcgtg cggagtttgt atcgcatgca gtcaacctag
    10921 gggccgctga gctgcgactt caaaaagtca atctggtcgg cgtcgctgct aattatcgca
    10981 tacagagaca gcatgagcag cggcaaggca atcgccaaaa atagcgtact catcggccag
    11041 tttgttcccg tgctcgtccg cgagcggaac gaaccttccc ccgtcgttcg cgcaccatac
    11101 cttgcgagca tgacagtaag ccgaggttcc tgctgccgcg cttccgcccc tggaccttcc
    11161 gtgccattga gattgccctc cgcacgagat agtattgcgc agctcggcgc atcggcgatt
    11221 tgctgccaga gaattctcca agccaatcgt aaatcgaggc agagtaaggg ctaaggaaaa
    11281 accgccccgg tagctcagag gatagagcac cggtttccta aaccgggggt cacaggttcg
    11341 aatcctgtcc ggggtaattt ttggagcaat catgtcgctg ttcatgtttt ccatcgcctt
    11401 gattgccgcg cctggggagg accagtccgt gaggctgaac cattcgctgc gcgaatatgt
    11461 cgcggctcgg gctgcggagt ttggggagat tccggaagag cggaaggagc agctgagcaa
    11521 gatcgccgac tacgtgcgcg ggcgaatcaa ggccgggcaa ccggcgcggc tagtcttcat
    11581 ctgcacccat aactcgcggc ggagccacct gtcgcagatt tgggcccaga ccgccgccag
    11641 ccactacggc gcagccaatg tggagacctt ttccggcggc acggaagcga ctgccttcaa
    11701 tccgcgggcg gtcaatgccc tgaagcgggc cgggttcatg atttccgagc cgccggccgg
    11761 cgagaacccg cgctatcagg ttcgcttcca cgacgacgcg ccgcccatgg agtgcttctc
    11821 caaggtctac agcgagagcc cgaacccgac cgcggatttc tgtgccgtca tgacctgctc
    11881 gcaagccgac cagaactgcc cgcaggtccg cggcgctgcg ctgcggatcg cgcttccgtt
    11941 cgatgacccc aaggccttcg acggcactcc cgaggaagcg gccaaatacg acgagcgctg
    12001 ccggcagatc gcccgcgaaa tgctgtttgt ttttgccagt ggcttgaagt aaggcggcgc
    12061 ggcgctgatc gacgatgcca gagccccagg gaccagcgcg tgaaaccgcc gccctgcgac
    12121 gctcacgccg gctgccggat cgtgccgacg tcgtggtggt tggcgccggc gcggccggcg
    12181 tctttgcgct gcgcgagctg gtccgcggag gcctgaccgc catcggtctg gaggcccgca
    12241 atcgtatcgg cgggcggatt ttcacgcgcc gcacgctggc ccggcatccc ctcgagctgg
    12301 gcgcggagtt cgtgcacggc cccaacaaca tcgttcacca actggctgcc gagtacggct
    12361 tgaccctggt tccgcatgcg gggacggcct attcctggtg ggacggccag ctcgtccccg
    12421 acgatcgcct gccgcggccg ccgctggcgg tgctccagga aatccgcggc ctggcggagc
    12481 ggcgcaggcg ctcgggaggc ggcgccatgc cgctggccga atttctcggc acgccggagc
    12541 tgtcggaaat cttcgagcgt tccgccgtca cccgccgcta tgtcgatcaa ctcatcaaga
    12601 acgatcattc ggtggagccg acgtcgctca cgctggaagg ctggctggag cccgacgtca
    12661 cgggcttcga ggccaactac cgcatcgacg agggctactc cgccttgctg caccgcgccg
    12721 cggaagcgga cgacctcgac atccggctca accggccggt gaagcggatc gagtgggaac
    12781 ccggccgcgt gacctgctat gccagcggcg cggcggtgcg gtcccgcgcg gcgatcgtca
    12841 ccttgccgct cggcgtgctg caacaggggg acgtcgagtt cgacgagccg ctgccgcggt
    12901 caaaggtgga ggcgctggag tcgttgctgc ccgggaaggc cttcaagatg cacacgagct
    12961 tccacgcgct gcggggcggc aggcccttct ggcccgcagg cctggctttc ctgaccagcg
    13021 cgctcgactc gcagctgcat tggccggcct cgcaagagcg ccgccgcggt cagcggcatt
    13081 tgctgacgca cctcgtgggc ggagacgccg ccgaccgctt tggacagcat cccgatccgc
    13141 cgcaagcgat gctcaaccag ctcgtgcata tgttcggcaa ttcgcggatt cgcgagctgt
    13201 tcgtccgcgc cgagtggcgc gcctggcaca cggaccccta cagccgcagc ggatattcgg
    13261 cgttccccag cgatgccgat cttggcgccc gccaggcgct cggccaaagc gtcatggaca
    13321 cgctgttctt tgccggcgaa gccgtgggcg tgaagggccg gcccggaaac gtggcgtcgg
    13381 tacacggagc gatcgagagt ggaatccagt gcgctcgcga cgcgattgct tccttgcgaa
    13441 cctagggcgc agcgactttt ttgggcctca gccaacacag cgggcgtccg gggtcaccgg
    13501 gcttgggcac acgatctccg caaactcttc gatcgagtcg atcgcctggc gatccatccg
    13561 ggctgcctca gcgggatcaa gcagcaccag caggcgcttg gagcccttca gcggcccggg
    13621 aagaatggaa tagaaccgat ccaggtaggt cgtgcggcga tagcaggcct gttccagctc
    13681 agccaataac gccgcggtta cggacgccgc gtcgcccggc tccaggatca cttgcagcgt
    13741 cgtgtgcgga ttctcgccga gcaactgctc gaccagctgg cgcgccgctg cgccatgctg
    13801 ccggaaatca gcggcgcgga gccacagggt gaaggcctgc gtccgctctg gcggccgagg
    13861 gaggccccct tcggccgcag tgcagccggc ggccgaccag ctttctccgt ccaggtcgat
    13921 ccgcgctacg ctggctggcg ccccgagggc cacagcccgt tccacgtcgg gaatcgccgg
    13981 ctcgggaagc ggatcgaact ccgtctcgaa cagctcctgg gcttcctcca tcagcagcga
    14041 aagatcggcc aggtcgagcg tcggagtctg cacgacgtag tacggcggcc gcgactggta
    14101 ctcgagcccc agctcgcggg cctcttgccg aaacgccgtt cccggcaata cggccaggtt
    14161 gaaaacctgc acctcgtcgg agagccggtt ctctcgcagg taggagaggc tgcggcggat
    14221 cgagtcgggc gtatctcccg gcaggccgat gatcaaatcc actttgacgc tgattcctgc
    14281 gtcctgcatc gctcgcacgc cgcgctcgaa ggcctggaga ttgttcttgc ggtccatcag
    14341 ctgctgcgcg agtggatccg tcgattgcaa cccgatctcg acgcgcgtga agttcgcggc
    14401 ccgcagatgc cgcgcgatat tggcggtgat tccctcggcc cgcagctccc caaagtatgt
    14461 gaactggcga tccggattgc actcggccag cagcttcaga aagccctcaa agtcggggcg
    14521 ctgattgagg gtcgggtcga gcagcaccac ctcgcgcgct ccccgccgcc gcgcatgctc
    14581 cagattcgcg accagcttct cgcgcgacgc aaagtacaag tcgtcgtagc tcttggggta
    14641 ataacagaat ttgcacttga agacgcagcc ccgcagcgtc tcgagcagca gcacccgctc
    14701 gtcggcggcg tcgagaatgc cggcgaggta cggcgaagag atctcgtcga gtttgctcag
    14761 cggccgccga aacgggggca attcgccgcg cgtggcgggc tccgcgctcc agtcgccgcg
    14821 gaccgtccgc gcgagagcgg gcgacgcgta caacccggga atcggcctgg cgggaatatc
    14881 gccggtcagc agttccgcca gcagctcgca gaacgtctgc tccccttctc cgatggcggc
    14941 gaaatcgact tccggccggg ccagcaccca gccattgtcg gcagtgattt ccggtccgcc
    15001 gacgagaatc cgcacttcag ggcgcgcctg cttcaccagc cgcgacacat cgagcgcccg
    15061 ctcaacgttc cacaggtaac aggtccagcc gacgagccac gcttcgcggc cctgaatctc
    15121 cgcagccagg ccctgatccg aaagctggtt ggcaagccgc gccggcagga tttcgatgtc
    15181 gtagctggat tccaggccgc ggagccgcgc cagcagcttc aaatatccgg cggccagcgg
    15241 aacgttttcc tcggccgggc tcattcccgg ctgcgggatg ggaagctgga tcagcagaac
    15301 ccggcgacgc tcggacatag cagaccgcga tagatgaatt ccaatcgttg cgaaaccagc
    15361 agcggcgcca cgagtccagg ccgcgtcgag cgcgaggttg tattgtacga gcaggcggct
    15421 cgatgcggcg gtcgcggatt acctggcatg agcgcataac gccaggtttt tcgcaggcgg
    15481 caatttgagt tgtaactgca atcgccgcag tgctttacga cgacattcgt cgatttccca
    15541 ttatgcgcat tatgcgcgcc ggcggctcct gcctcgtctt tgctgcagcg aggggggacc
    15601 ctcgcggaat tgcgttacca gggaggaccc tggcaacgag ggagtgctta gccgccagac
    15661 ctgcatctta cgcgggccgc gctggctcgc ctgcccgccg acgagcccgt ggaagacgaa
    15721 gaggaa
//
LOCUS       DASRJB010000582         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3831119, whole genome shotgun sequence.
ACCESSION   DASRJB010000582 DASRJB010000000
VERSION     DASRJB010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3831119"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFB80_15320"
     CDS             complement(<1..1075)
                     /locus_tag="VFB80_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HZN35197.1"
                     /translation="MTNHPLLANLTSAEGLHRMSVAELSQLGGEIRDVLCNLLEERTA
                     HFASNLGVVELALALHSVFDFRRDRLIWDTGHQIYPHKLITGRYRGFTSIRTKGGLMG
                     YPNPHESDYDLFMTGHAGSSLATLSGLRSGHDLLGEADRKTVAVIGDGAFPSGIVFEA
                     LNNISGLNQDVLVILNDNKMSICPRVGGVASYLDRLRTSPFYSGLKQEVVRILENVPL
                     LGDPVERFLAQLKEGVKAGLHGGMLFEELGLRYLGPIDGHNIALLRKYLLMVKNLKGP
                     ILLHVVTEKGHGFTPAAKDPVFFHTPPAFTQENGNPVPKKSGGSPAYTNVVSDAIRQR
                     MLDNPHVTVLTAAMCQGNKLERIR"
     gene            complement(1127..2023)
                     /locus_tag="VFB80_15325"
     CDS             complement(1127..2023)
                     /locus_tag="VFB80_15325"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZN35198.1"
                     /translation="MTAAAETLDFAELSRSLRRRIDDALASYATYGADCPPHLAEAIR
                     HSLLAPCKRLRPQLVLMSCQACGYDAWQALPAACAVEMIHTYSLIHDDLPAMDDDDLR
                     RGRPSCHAKFGEATAILAGDALLAQAFEVLARGLPPDVAARCCGELAAAAGAENLVGG
                     QEDDLRAQFADLGIEQLERIHRRKTGAMIRVSLRLGGIVGGADERQLAALDTYGGKLG
                     LAFQIVDDLLDLQGDAASLGKRANKDTGRGKLTFPGLLGAAESRRRAERLIKEAIAAL
                     APLGPRAAALESLARYVLERNS"
     gene            complement(2113..2448)
                     /gene="xseB"
                     /locus_tag="VFB80_15330"
     CDS             complement(2113..2448)
                     /gene="xseB"
                     /locus_tag="VFB80_15330"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZN35199.1"
                     /translation="MSFEFSLAELEAIVRDLEDGKLGLAEALARYEEGIKHLKHCYAL
                     LAQAEQKIELLTRVAEDGSPVTRPFTDESEPPPRAAGKQRTKPKPKQPADDELPSFRR
                     DIDDGTGRT"
     gene            complement(2480..2815)
                     /locus_tag="VFB80_15335"
     CDS             complement(2480..2815)
                     /locus_tag="VFB80_15335"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZN35200.1"
                     /translation="MAKKQHRIIWTINALDQLERIKQFIARDSPMNALNFVRRLYRHA
                     RRLKTLPYSGAPVPEFGDPETREIIYRNYRIIYEVVLSTVRVLAVHHGAKLLGRQHLA
                     KSRRTDADE"
     gene            complement(2733..2993)
                     /locus_tag="VFB80_15340"
     CDS             complement(2733..2993)
                     /locus_tag="VFB80_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35201.1"
                     /translation="MKLTNKQKIEKLVARMPADISWDEALQRIRLLRSVEIGREQAER
                     GEVIDDEDVEAWLMNDGEEAAPDNLDDQRSRPARKNKAVHRT"
     gene            complement(3066..4286)
                     /gene="xseA"
                     /locus_tag="VFB80_15345"
     CDS             complement(3066..4286)
                     /gene="xseA"
                     /locus_tag="VFB80_15345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZN35202.1"
                     /translation="MTATAPQPPRVLSVSQLTALIQGSLESTFASVWVSGEVSEISRP
                     QSGHVYFTLKDEGAQLRGVLWRSSAVRLRFRLEEGQQVLCLGDLTVYPPRGAYQLDVR
                     RVEPQGQGALQLAFQQLQRRLAAEGLFDPARKRPLPVLPRRVGIVTSPSGAAIHDFLQ
                     VAARRCCGVRIFVIPARVQGDGASGEIVHGIQLANRLHPPLDVLVVGRGGGSLEDLWS
                     FNEEAVVRAIHASRVPVVSAVGHEVDVTLADLAADVRALTPSEAAERVFPAAEELTQR
                     LTAIQRRLIAILRARASAARRHLEQLARSRILRNPKSLVFDLSRRLDEHDQQALRAIA
                     RRLDLARDRLTASTARLESLSPLAVLARGYSVTTDDAGKILKSTAALRAGDTVHTRLA
                     TGGFTSRVGAVESE"
     gene            complement(4294..5133)
                     /gene="dapF"
                     /locus_tag="VFB80_15350"
     CDS             complement(4294..5133)
                     /gene="dapF"
                     /locus_tag="VFB80_15350"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZN35203.1"
                     /translation="MRFTKMHGIGNDYIYVNCFAEPLPKNPAELARRIADRHFGVGAD
                     GLILICPSDSGADARMRMYNTDGSEAEMCGNGLRCVAKYVHDHGIKRADRLTIQTGRG
                     NLTVDLEIRGGKAERICVNMGQPILEGTKIPTKLVGNPPLDVPLVVGGQTLQVTCVSM
                     GNPHCVTYVERASDELVLGLGPKVEQHAMFPARVNVEFIEIISPTEVRQRTWERGSGE
                     TLACGTGASAVCVAGVLTGRTQRKIVNHLRGGDLELEWRESDGCVYKTGPAVEIFSGD
                     WPT"
     gene            complement(5226..5645)
                     /locus_tag="VFB80_15355"
     CDS             complement(5226..5645)
                     /locus_tag="VFB80_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35204.1"
                     /translation="MAAQAILGAALGTAAIAVGRGIAAATGDGLSFAAELLKAAGGQP
                     SEMDDNAGPDELTQRISALADRFRRHLAAAGIKLLEPVELISDGLGGIAVAGVHPQRA
                     EIEEALAADLTLESEFNRLAVEYGEGLPSVVVSNEAQ"
     gene            5765..>7306
                     /locus_tag="VFB80_15360"
     CDS             5765..>7306
                     /locus_tag="VFB80_15360"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZN35205.1"
                     /translation="MPRHPRRATFSAPLGQPQLAREERRALGAYYTPPEIVRLIVELT
                     LEPLLAERPPEPPRVIDPACGAGEFLAASAERLARVYGSRTKVYGLDLAAAAIQAARR
                     RLGAAGVVRQGNALLDDQPADGTFDVVIGNPPYLSIRQLAKSLPAEEIEQLRQRFRTA
                     RGNFDLYVLFIERALALLQPGGRCGLIVPNKWATLDYARTCRELLLEQATIEHVVDLS
                     NERVFQGASVYPHVLVFRKQPAPRRHVVTFRAEDGVRRIVQSSLSPAAFSFSEPLDVE
                     SRVATKPLGELAKLSCGTAGYAAQRIARRLVDDSSPHAPSEESHLAERDDYCDFITSG
                     NIDRYAVRLGGVRYLGRTYERPRLPLDAPEITPAKRHLFESRKIVIAGMSRRIEAAWD
                     ERGLALGVQVFAASEPKVDAFYLLALLNSKLLSYLFATRFAAKRLAGGYLAINNGQLE
                     RLPVVVEQASPLAHAGNKRGRLFYDVSRRLAVLGRLLSGPAGNPPHEAEADRLVYQLY
                     RLSRAE"
BASE COUNT         1164 a         2451 c         2406 g         1285 t
ORIGIN      
        1 cgcgaatccg ctccagttta ttcccctggc acatcgcggc ggtgagcacg gtgacgtgcg
       61 ggttgtccag catccgctgg cggatcgcgt cgctgacgac gttggtatag gccgggctgc
      121 cgccggactt tttcgggacc gggttaccgt tctcttgcgt gaaggcgggc ggcgtatgga
      181 agaagacggg gtccttggcg gcgggcgtga agccgtgccc tttttcggtc acgacgtgca
      241 gcaggatcgg ccccttgagg ttcttgacca tcaagaggta cttgcgaagc agcgcgatgt
      301 tgtgcccgtc gatcgggccg aggtaccgca ggcccaattc ctcgaacagc atgccgccgt
      361 gcaggccggc tttgacgccc tccttgagct gggccaggaa ccgctcgacg ggatcgccca
      421 gcagcgggac gttctcgaga atgcggacga cctcttgctt gaggccggaa tagaacgggc
      481 tggtccgcag ccggtcgagg tagctcgcga cgccgccgac gcgcgggcag atcgacatct
      541 tgttgtcgtt gaggatgacc agcacgtcct ggttcaggcc gctgatgttg ttgagggctt
      601 cgaacacgat gccgctgggg aacgcgccgt cgccgatgac ggctacggtc ttgcggtccg
      661 cttcgcccaa gaggtcgtgg ccgctccgca ggccggagag cgtcgccagg ctgctgccgg
      721 cgtggcccgt catgaacagg tcgtagtcgc tctcgtgcgg attgggatag cccatcagcc
      781 cgcccttggt gcggatcgac gtgaagccgc ggtagcggcc ggtgatcagc ttgtgcgggt
      841 aaatctgatg gccggtgtcc cagatcaggc ggtcgcggcg gaagtcgaag acgctgtgca
      901 gggccagggc caattcgacg acgcccaggt tcgaggcgaa atgggccgtc cgctcctcca
      961 gcaggttgca gagcacgtcg cggatctcgc cgcccagctg cgacagctcc gcgaccgaca
     1021 tgcggtgcag gccctcggcc gaggtcaggt tcgcaagcag cgggtggttg gtcatggtgt
     1081 ggtagcccgc cactccgtgg cgggtttccc gcgatggaat cgcgggctac gaatttcgtt
     1141 ccagcacata ccgggccagc gactccaggg ccgccgcccg cggacccaag ggcgccagag
     1201 cagcgatcgc ttccttgatc agccgctcgg cccgccggcg gctctcggct gctccgagca
     1261 gtccgggaaa cgtcagcttt ccgcgaccgg tatccttgtt ggcccgcttg ccgagcgacg
     1321 ccgcgtcgcc ctgcaagtcg agcaggtcat ccacaatctg aaaggccagg ccgagcttcc
     1381 cgccataagt atctagcgcg gccagctgcc tttcatccgc accgccgacg attccgccca
     1441 gccgcaggct gacccgaatc atcgcgccgg tcttacgacg atgaatgcgt tccagttgtt
     1501 cgattccgag gtcggcgaat tgtgctcgga ggtcgtcctc ctgcccgccg acgaggttct
     1561 cggcgccggc cgccgccgcc agctcgccgc agcagcgggc ggcgacatcg ggcggcaggc
     1621 ccctggccag cacctcgaag gcctgggcca gcagggcgtc gccggcgagg atcgccgtcg
     1681 cctcgccgaa cttcgcatgg cagctcggcc gcccgcggcg gaggtcgtcg tcgtccatcg
     1741 ccggcaggtc gtcgtggatc agcgagtacg tatgaatcat ctccaccgca cacgcggcag
     1801 gcagcgcctg ccacgcatcg taaccgcagg cctggcacga cattaacacc aactgcggcc
     1861 gcaggcgttt acacggcgcc agcaggctgt ggcggatggc ctcggccagg tgcggcgggc
     1921 aatccgcccc gtaggtcgca tagctcgcca gcgcgtcgtc gatccgtcgg cggagcgacc
     1981 ggctcagttc ggcaaagtcg agcgtctccg cggctgcggt cattcctgat gctgctggag
     2041 agttgcaagc tgctaccggc aaaggacttg cgtcagtcgc ttccggagca gacgcacata
     2101 ggcgacacca ttctaggtcc gaccggttcc gtcgtctatg tcgcggcgga agctgggcag
     2161 ctcgtcgtcg gcgggctgct ttggcttggg cttagtgcgc tgtttacccg cggcccgcgg
     2221 cggaggctcc gactcgtcag tgaatggcct ggttaccggc gagccgtctt ccgccacgcg
     2281 ggtgagcagc tcgattttct gctccgcctg cgcgagcaga gcgtagcaat gcttgaggtg
     2341 ctttatgcct tcctcgtagc gggccagcgc ctcggccagc ccgagcttgc catcctccaa
     2401 atcgcgcacg atggcctcga gctcggcgag cgagaattcg aaggacatcg gctgtcggct
     2461 cgacgagtct gccggctgat cactcatctg cgtcagtccg tcgagatttg gccagatgct
     2521 ggcgaccgag cagttttgca ccgtgatgca cggcgaggac tctcaccgtg gacagaacaa
     2581 cttcatagat gatccggtag ttgcggtaaa taatctcgcg cgtctccgga tcgccgaatt
     2641 ctggtaccgg tgcgccagaa taggggagcg tcttcaagcg acgagcgtgc cggtataacc
     2701 gccggacaaa gttcaatgca ttcatcggcg agtcacgtgc gatgaactgc tttattcttt
     2761 cgagctggtc gagagcgttg atcgtccaaa ttatccggtg ctgcttcttc gccatcgttc
     2821 atcaaccagg cttccacatc ttcatcgtcg atgacttcgc cgcgctcggc ctgttcgcgg
     2881 ccgatctcca cgctccgcag caggcgtatt cgctgcaacg cttcgtccca cgaaatatca
     2941 gccggcatcc gcgccaccag tttctcgatc ttctgcttgt tcgtcagttt catcacttcc
     3001 tccgacgacg gtgcaacaac cgcctcactc cattatggcg tcaattggcc tggggcggca
     3061 acgattcact cggattcaac cgctccaacc cggctggtga acccgccggt agcaagcctt
     3121 gtatggacgg tatcacccgc gcggagggcg gcggtggatt tcaggatctt gccggcatcg
     3181 tcggtggtca cgctgtagcc gcgggcgagg acggcgagag ggctgagcga ttcgagccgg
     3241 gcggtgctgg cagtgagccg gtcgcgagcc aggtcgagcc ggcgggcgat cgcgcgcagc
     3301 gcctgctggt cgtgctcgtc gaggcggcgc gagaggtcga aaacgaggga cttcggattg
     3361 cgaagaatgc gcgaacgggc gagctgctcg agatggcggc gggccgcgga ggcccgggct
     3421 cggagaatcg cgatcaggcg gcgctggatc gccgtcagcc gctgcgtcag ttcctcggct
     3481 gccgggaaca cgcgctcggc ggcttcgctg ggcgtcagcg cacggacgtc ggccgcgagg
     3541 tccgccagcg tgacatccac ctcgtggccg accgccgaga cgaccggcac gcgcgaggcg
     3601 tggatcgccc gcacgacggc ctcttcgttg aagctccaca gatcttccag gctgccgccg
     3661 ccgcggccga cgacgagcac gtcgagcggc gggtggagcc ggttggcaag ctggatgccg
     3721 tggacgattt cgccggaggc gccgtccccc tgcacgcggg ccgggatgac gaagattcgc
     3781 acgccgcagc accgccgcgc ggcgacctgg aggaaatcgt ggatcgctgc gccagagggg
     3841 ctggtgacga tgccgacccg ccgcggcagc accggcaggg gccgcttgcg ggcgggatcg
     3901 aacaggccct ccgccgccag tcgccgctgc agctgctgaa acgcgagctg gagcgcgccc
     3961 tgtccctgcg gctccacgcg ccgcacgtcg agctgatacg ctccccgcgg cggatagacc
     4021 gtcaggtcgc cgagacagag gacctgctgg ccttcctcca ggcggaaccg cagccgcacg
     4081 gcgctgctcc gccagagaac cccgcggagc tgcgcccctt catccttgag cgtgaaatag
     4141 acgtgccccg attgcggccg cgagatttcg ctcacctccc cgctgaccca gaccgacgcg
     4201 aaggtcgatt cgagcgagcc ctggatgagc gccgtgagtt gcgagacgct caggacgcgc
     4261 ggaggttgcg gagcggtggc cgtcatggcg cgcctacgtc ggccaatcgc ccgagaagat
     4321 ttcaactgcc ggtccggtct tatagacgca gccgtcggac tcgcgccatt cgagctccag
     4381 gtcgccgccg cggagatggt tgacgatctt tcgctgcgtg cggccggtga gcacgccggc
     4441 gacgcaaacg gcgctggccc ccgtgccgca ggccagcgtc tcaccgctgc ctcgctccca
     4501 ggtccgctgg cggacttccg tcggcgagat gatctcgatg aactcgacgt taacccgtgc
     4561 cgggaacatc gcgtgctgct caaccttcgg accgagtccc aacaccagct cgtcgctggc
     4621 ccgctcgacg tacgtcacgc agtgcgggtt gcccatcgag acgcaggtga cctgcagcgt
     4681 ttggccgccc acgaccagcg ggacatccag cggcggattg cccacgagct tcgtcgggat
     4741 cttcgttcct tccaggatcg gctgccccat gttcacgcaa atccgctcgg ccttcccgcc
     4801 gcgaatttcc agatcgacag tcaggttgcc gcggccggtc tggatcgtca gccggtcggc
     4861 ccgcttgatg ccgtggtcgt gaacgtactt ggcgacgcac cgcaggccgt tgccgcacat
     4921 ctcggcttcg ctgccgtcgg tgttgtacat ccgcatccgc gcgtcggccc cgctgtcgct
     4981 cgggcagatc aggatcagcc cgtcggcccc gacgccgaaa tgccggtcgg cgatccgccg
     5041 ggcaagctcc gccgggttct tgggcagcgg ctcggcaaag caattgacgt agatatagtc
     5101 attgccgatg ccgtgcatct tggtgaaacg catggggcca gtctcgcggc gcgggagcga
     5161 agggctgctc tgattctacc gccgccctaa agccgcgacc attggtcgcg ctgcacgagc
     5221 tggcgtcact gcgcttcgtt cgaaacgacg acgctcggca ggccttcgcc atactcgacg
     5281 gccagtcgat tgaattcgct ctccagcgtc agatcggcgg caagcgcctc ctcaatctcg
     5341 gcccgctgcg gatgtacgcc ggcgacggca attccgccca gtccgtcgct gatcagctcc
     5401 accggttcca gcagcttgat gcccgcggcg gccagatgcc ggcggaagcg gtcagccagc
     5461 gcgctgatcc gctgcgtcag ctcgtccggc ccggcgttgt cgtccatttc agatggctgc
     5521 ccgccagcgg ccttgagcag ctcggcggcg aacgacaacc cgtcgccagt tgccgctgcg
     5581 atgccccggc cgacggcaat cgccgctgtg ccgagcgccg cgccgagaat tgcctgtgcc
     5641 gccatgtttc gcctcgtgag ccgccgcctg ctagcagcgg caagccgtgc cgcattttcc
     5701 cgctccgttg atcgacagaa tcgcgctgcg gcttgagctc aaattgccta cgattcgcgg
     5761 ccccatgccg cgtcacccaa ggcgagcgac gttttccgca ccgctgggcc agccccaatt
     5821 ggcccgcgaa gagcgccgcg cgctgggagc ctattacacg ccgccggaaa tcgtgcggct
     5881 gatcgtcgag ctaacgctcg agccactgct ggccgagagg cctccagagc cgccgcgcgt
     5941 gatcgacccg gcctgcggcg cgggagagtt cctcgccgct tcggccgagc gactcgcacg
     6001 agtctacggc agtcgcacca aagtctacgg tcttgacctc gccgcagccg ccattcaggc
     6061 cgcccggcgg cgcttagggg ccgccggcgt cgtccggcaa ggcaacgcac tgcttgacga
     6121 tcaaccggcg gacggcacgt tcgacgtcgt catcggcaac ccgccctatc tgagcatccg
     6181 gcaactcgcc aaaagcctgc cggcggagga gatcgagcaa ttgcggcagc gctttcgcac
     6241 agcccgcggc aattttgacc tctacgtcct gttcatcgag cgggcgctgg ccctgcttca
     6301 accgggtgga cgctgcgggc tgatcgtgcc caacaaatgg gccacgctcg actacgcccg
     6361 gacctgccgc gagttgctgc tggagcaagc gacgatcgaa cacgtggtcg atttatcgaa
     6421 cgagcgcgtc ttccagggcg ccagcgtcta tccgcacgtg ctggtcttcc gcaagcagcc
     6481 cgcgccgcgg cggcacgtgg tgacgtttcg cgcggaggat ggcgtgcgcc gtatcgttca
     6541 gtcctcgctg tcccccgcgg cgttctcgtt cagcgagccg ctcgacgtcg agtcgcgcgt
     6601 ggcgacgaag ccactgggag agctggcgaa gctgtcgtgc ggaaccgcgg gctatgcggc
     6661 gcagcggatc gcgcggcgat tggtcgacga cagtagtcct catgctccga gtgaggaatc
     6721 gcatctcgcg gagcgagatg actactgtga tttcattacc agcggcaaca tcgaccgcta
     6781 cgcggtccgg ctgggcggcg tccgttatct cggacgcacc tacgagcgcc cgcggttgcc
     6841 actggacgcg ccagagatca ctccggccaa acggcacttg ttcgagtcac gcaagatcgt
     6901 gatcgccggg atgtcgcggc ggatcgaagc cgcctgggac gagcgcggac tcgcgctggg
     6961 cgtgcaagtc ttcgcggcta gcgagccaaa ggtcgatgcg ttctatctac tggccctgct
     7021 caattcgaag ctgctctcgt acctgttcgc cacgcggttc gccgccaagc gcctggccgg
     7081 cggctacctg gcgatcaaca atgggcagct ggaacgactg cccgtcgtcg tagagcaagc
     7141 gtccccgctt gctcacgcgg ggaacaagcg aggacgcttg ttctacgacg tcagccgccg
     7201 gctggccgtt ctcggccgcc ttctctccgg gccggccgga aacccgcccc acgaggcgga
     7261 agccgaccgg ctcgtgtatc aactctatcg cttgtccagg gccgag
//
LOCUS       DASRJB010000583         7104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1016946, whole genome shotgun sequence.
ACCESSION   DASRJB010000583 DASRJB010000000
VERSION     DASRJB010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016946"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFB80_15365"
     CDS             <1..200
                     /locus_tag="VFB80_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35206.1"
                     /translation="AGFGFSGGFGFTSTASGSAFTDAAAYRDCYRVNMKVQEFPRRGQ
                     PNPMNPAAPQPEPAPAAADSL"
     gene            744..1082
                     /locus_tag="VFB80_15370"
     CDS             744..1082
                     /locus_tag="VFB80_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35207.1"
                     /translation="MKNVFVRMWREEDGVLSFEWVLLVTLLTIGIVGGLAAARDAIID
                     ELGDVAQAMLALDQSYTIAFPLLVIVHDPAVSSASDSSFTDALLFTDCSRATTPTGQP
                     AQNDAVPDGA"
     gene            1181..1534
                     /locus_tag="VFB80_15375"
     CDS             1181..1534
                     /locus_tag="VFB80_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35208.1"
                     /translation="MRRICTAMWRDQSGVLSFEWTLLVTLVVLGVVGGLAATRDAIID
                     ELGDAAQAMLALDNSYTITFPLEILIDLNVLAPPGPAAVGFASDSSFTDALAFTDCSR
                     ATSPTGQPAQSDDPS"
     gene            1603..1941
                     /locus_tag="VFB80_15380"
     CDS             1603..1941
                     /locus_tag="VFB80_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35209.1"
                     /translation="MTHNLGKIWREEDGVLSFEWTLLLTLLVLGIVGGLAAARDAIID
                     EMGDVAQAMLALDHSMTIAFPLQVLVHNSSDPSSASDSGFTDALVYTDCTRNDAVPGQ
                     APQFVEDTDS"
     gene            1994..2329
                     /locus_tag="VFB80_15385"
     CDS             1994..2329
                     /locus_tag="VFB80_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35210.1"
                     /translation="MKPALQRLWIEEDGVLSFEWVMLLTLLVIGIVGGVAAARDAIID
                     ELGDTAQVMLAVDQSYHIGFPLLVQLHDATVSSASDSDFADALLYADCERSTFIAQDF
                     VDDGIPDGA"
     gene            2435..2776
                     /locus_tag="VFB80_15390"
     CDS             2435..2776
                     /locus_tag="VFB80_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35211.1"
                     /translation="MNCLLRMWREEDGVLSFEWTLLLTLLVIGVVGGLAAARDAIIDE
                     LGDVAEAAQNIDQSYSLSPISFTFGMMGDPFFFTFTSAGSDFTDDAGIFTDCARATTP
                     TGEGPVSDGGS"
     gene            2822..3130
                     /locus_tag="VFB80_15395"
     CDS             2822..3130
                     /locus_tag="VFB80_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35212.1"
                     /translation="MQQILQQAWREDDGVLSFEWTIITVVIVFGIVGGFAAIRDALID
                     ELGDMAEAVIEIDQSFSFSGLPAFGIPPSSFTDPPGSVTDCGRQTTPLGHPGSSDAPA
                     "
     gene            3336..3866
                     /locus_tag="VFB80_15400"
     CDS             3336..3866
                     /locus_tag="VFB80_15400"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN35213.1"
                     /translation="MHFGYDLAACVAVFTAACAVVDYRTKKIPNWLTVPAAAIGLAYH
                     LLAPGGSGPLVALGGFAIGFGLLIVPWLLGGGGMGDVKMLAALGVWLGPLGILASFGL
                     GAMLAAFGMIGVLIGSTFSDGFSATRKRFAHATASGASAAATPPKKARRVLPFAVPMA
                     LGTWLVLGWMLLKSHG"
     gene            4117..5151
                     /gene="cpaB"
                     /locus_tag="VFB80_15405"
     CDS             4117..5151
                     /gene="cpaB"
                     /locus_tag="VFB80_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZN35214.1"
                     /translation="MKRISPATVTFGVMAIVLGLVAAYVVRQALQKPPVVEKPVPPPP
                     APEVQYLVFAKNVIPKNTRLTAADLFMGAVPKTEKKPEGMFHGINLVEGRITKETIAP
                     GKIIREQNLLGLDESLPDLSNRLPEGHRAVTIIVQGADTGGKRLGEGDHIDIALTVEG
                     SHPDLGEVMTRTLMHNVLIVDAAASRPLMRGTRRTTDLNGSSITVAVTPVDANKLIVA
                     QRTGILQATLVSAKDNAPIGASADPVSRRQLLGLKEIVPPKKYTVEKWSGNSVQILEM
                     SNDRIRESREISAGRREVPVSQPIPAPANDQVGRIPTGVKVPDVAYELGEEPLAALAP
                     VAPEGAKEVK"
     gene            5258..6697
                     /locus_tag="VFB80_15410"
     CDS             5258..6697
                     /locus_tag="VFB80_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZN35215.1"
                     /translation="MFANAFRPAGERNGNGNGNGHGHAETLMQAENLEAEFQRLKTGI
                     HRELLDSLDLSRITGLSDEELRHDIRQLAEGMLRTRSRKLPAIDEERLIEELIAESFG
                     LGPLEPYMQDPDVSDILVNGPHEVYVERLGRLQETNTVFADDEHLLQIIQRIVARIGR
                     RIDEHQPMVDARLPDGSRVNAIIPPLALRGPVLSIRRFGHRPLQIADLLVRGSVCPEM
                     LKLFEMAIEGRINVMISGGTGSGKTTLLNNLSKFIPANERLVTIEDSAELRLQRKHVV
                     KLETRPKSAEGTAEVSQRDLVRNALRMRPDRIILGEVRGGEALDMLQAMNTGHEGSLT
                     TIHANDTRDALSRLEVMVSMAGFDLPVGVTRRYIASAITLVIHLARLKGGVRRVMRVS
                     EITALESGDYAIQDLFGFQQTGVDDRGIARGHFFATGHMPVFARRLIEQGIDLPTKLF
                     EPRQLTPDSSIGPMSVTAAGGAAPLEEMA"
     gene            6694..>7104
                     /locus_tag="VFB80_15415"
     CDS             6694..>7104
                     /locus_tag="VFB80_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35216.1"
                     /translation="MMIFANLAVVGVTFASVTMILAAVGMFIRDLFAGSASTARQRLT
                     FAPEEPAGDINRSFFHLVEESGTTMDMSTALAMVIGAGIVGVTIPLALFDNLLAAAGG
                     LVLGAAIPILYLSVVRWWRLGGMRKKLPYALQAVA"
BASE COUNT         1256 a         2396 c         2259 g         1193 t
ORIGIN      
        1 tcgcgggctt tgggttcagt ggaggttttg gcttcacgtc cacggcctcg gggtcggcgt
       61 tcaccgacgc ggcggcctat cgggattgct accgcgtgaa catgaaggtc caggagttcc
      121 cgcggcgcgg ccagccgaac ccgatgaatc ccgccgcccc gcagcccgag ccggctccag
      181 ctgccgcgga ctccttgtaa cgcacagtcg tcaaactcct taaacacgga acgcggaaac
      241 tcggccacgg ggagtgcccg ccctgcggcc gatccaccag gtttgacctt cgcccttcgt
      301 tcttcgcccg ccagcgcgag tgccccgcgc tggcggcatc caggaagaac aagcggcgca
      361 ctccgagctg cctggcagca cgggaaacac gacgcctcgc agcgagcgat ccgctggctg
      421 cgaggcgttt cttttttgcc acacgtttgt gcttgtgaac atattgcctt ggcgggcaag
      481 cgctgcgtgt ttcagcgcca caacatcgcg cggcggcgca agagcgcggg cctgcggccg
      541 cgaccgctcg taaatcacgt tccgccaacg gctaagcctc agcgcgcagc gattggtcaa
      601 cattttttcg cgcgcggcac atgaggtgca tttatggccg cacgagaaag tcggcgcttc
      661 gggcacaaca agcgccggcg gagcgcacct atgtgggggt gctccacacg tgttttccgt
      721 gttcctggta caaggagttt cgaatgaaga acgtgttcgt gcgtatgtgg agggaagagg
      781 acggcgtcct ctccttcgaa tgggtcctgc tggtgaccct tctcaccatc ggcatcgtcg
      841 gcggcctggc ggctgcccgc gacgccatca tcgatgagct gggcgacgtg gcccaggcca
      901 tgctggcact cgatcagtcc tacacgatcg cgttcccgct gttggtcatc gtccacgatc
      961 cggccgtcag ctcggcgagc gacagctcgt tcaccgacgc gctgctcttc acggactgca
     1021 gccgggcgac cacgccgacg ggccagccgg cccagaacga cgcggttccg gacggcgctt
     1081 agtccaattg cgcgcagtaa ccgagtccac tcggctggtc cgccagccga gtgggctccc
     1141 ttttggtcag tgctggtgga acggagagga gtcagaggca atgcgacgaa tttgcacagc
     1201 gatgtggcgg gaccagtccg gcgtgctctc gttcgaatgg acgctgctgg tgacgctagt
     1261 cgtgctcggc gtagtgggcg gcctggcggc gacccgcgac gcgatcatcg acgagctggg
     1321 cgacgccgcc caggcgatgc tggcgctgga caactcctac acgatcactt tcccactgga
     1381 aatcctgatc gacctgaacg tcctggcgcc tcccggcccg gcggccgtcg ggttcgccag
     1441 cgactcgagc tttaccgacg ccctggcctt caccgactgt tcccgcgcga cctcgccgac
     1501 cggacaaccg gcccaatcgg acgacccctc gtagcgcggc tggcgaacga acacgcaaca
     1561 cacacctggt tctggaaaaa cacgtacacg gggaaacaag aaatgacgca caaccttggc
     1621 aagatctggc gagaggagga cggcgtgctg tccttcgaat ggaccctgct gctcaccttg
     1681 ctcgtgctcg gcatcgtcgg cggcctggcg gcggcccgcg acgcgatcat cgacgaaatg
     1741 ggggacgtcg cgcaggcgat gctggccctc gatcactcga tgacgatcgc ctttccgctg
     1801 caggtgctcg tgcacaacag cagcgatccc tccagcgcga gcgacagcgg cttcaccgac
     1861 gccctggtct acaccgactg cacgcgcaac gacgcggtgc ccggacaggc cccgcaattc
     1921 gtcgaggaca ccgactccta agccgaaaac gccgaaccaa ttcccagcca ccagaagaga
     1981 aagccaaact ccaatgaaac cagcactcca gcgcctgtgg atcgaagagg acggcgtcct
     2041 gtcgtttgaa tgggtgatgc tcctgacctt gctcgtcatc ggcatcgtcg gcggcgtcgc
     2101 ggccgcccgc gacgccatca tcgacgagct gggcgacacg gcccaggtca tgctggccgt
     2161 ggaccagtcg taccacatcg ggttcccgct gctggtccag ctgcacgacg cgaccgtcag
     2221 ctcggccagc gattcggatt ttgccgacgc gctgctttac gccgactgcg agcgctcgac
     2281 gttcatcgcc caggacttcg tggacgacgg catcccggac ggcgcctagt ttgtgacaca
     2341 gccaaacagg cggcggcgca attttggcgt gcgattcgca caccggtgcg ccgctgcctc
     2401 ggaatacgag atctcatcta ctttagaggc tcccatgaat tgcttgctgc gaatgtggcg
     2461 cgaagaggac ggcgtgctct cgtttgaatg gacgctgctc ctgacgctgc tggtgatcgg
     2521 cgtggtcggc ggcctggccg cggcccgcga cgcgatcatc gacgagctgg gcgacgtggc
     2581 cgaggccgcg cagaacatcg accagagcta cagcctgtcg ccgatcagct tcaccttcgg
     2641 catgatgggc gatccgttct tcttcacgtt cacctcggcg ggcagcgact tcacggacga
     2701 tgcgggcatc ttcaccgact gtgctcgcgc cacgactccg acgggggaag gccccgtgtc
     2761 ggacggcggt tcgtaatcgg ccgccgagga atttcgcgac ttgacttccc ggagcaacga
     2821 cgtgcaacag atcctgcagc aagcctggcg agaggacgac ggcgtcctct cgttcgaatg
     2881 gacgatcatc acggtcgtga tcgtgttcgg catcgtcggc ggattcgccg cgatccgcga
     2941 cgcgctcatc gacgagctcg gcgacatggc cgaagccgtg attgaaatcg accaatcctt
     3001 ctcgttctcg gggctgcccg cgtttggcat tcccccgagc agcttcaccg acccaccggg
     3061 ctcggtgacc gattgcggtc gtcagaccac gcccttgggt cacccgggct ccagcgacgc
     3121 cccggcctga tttcgaaact cggtgagaaa gcgcttgccg cgtggctcgg ttggagaccg
     3181 gccacatggg cggtgaaagt ttccgcacga cgcgtcggca caatcgtcaa ctcacgcctc
     3241 acgggggccc cgtattactc gcggcagcgt gggcggcacg tgatttgttg ctggacggtt
     3301 cggcgattga gtttccaccc tggagcgctg ttgtgatgca ttttggctac gacctggcgg
     3361 cctgcgtggc tgtcttcacc gccgcgtgtg cggtcgtcga ttaccggacc aagaagattc
     3421 ccaactggct gaccgtgccc gcggcggcga tcgggctggc ctatcacctg ctcgcacctg
     3481 gcggcagcgg cccgctggtg gccctgggcg gcttcgcgat cggcttcggc ctgctcatcg
     3541 tgccgtggct gctgggcggc ggcggcatgg gtgacgtgaa aatgctcgca gccctgggcg
     3601 tgtggctcgg gcctctagga attctcgcct cattcggcct gggcgcgatg ctcgccgcct
     3661 tcggcatgat cggcgtgctg atcggcagca cgttcagcga cggcttctcg gccacgcgga
     3721 agcgttttgc ccacgcgacc gccagcggcg cgagcgccgc cgccacgccg ccgaagaagg
     3781 cccgccgcgt gcttcccttt gcggtgccga tggccctggg cacctggctc gtcctgggct
     3841 ggatgttgct caaaagccac ggctaacgga cgtttccgaa tcgaaacagc gcgcgtgcaa
     3901 actggcaaca ttcgcccgtt aaccgaatga accacctccc gactaactcc aggaatttca
     3961 ggccgacgca gccggcaagt ccggttagcg cggcccccag cggcagcggc atttcatttg
     4021 cgtacctgcc tcgcaacgcc tccgcgcacc gtgccggcgc tcggcgggct taacacgacg
     4081 aaccacccgg catccgcagc gatgaggaga acctaagtga aacgaatcag tcccgcgacc
     4141 gtgaccttcg gcgtcatggc gatcgtgctc ggcctcgtgg ccgcgtacgt cgtccgccag
     4201 gctttgcaaa agccgcccgt cgtcgagaag cccgtgccgc cgcccccggc gcccgaggtg
     4261 cagtatctgg tcttcgccaa gaacgtgatt cccaagaaca cgcggctgac cgcggccgac
     4321 ctgttcatgg gcgccgtgcc gaagaccgag aagaagccgg agggcatgtt ccacggcatc
     4381 aacctggtcg aaggccgcat cacgaaggaa acgatcgccc ccggcaagat catccgcgag
     4441 cagaacctgc tgggactcga cgagagcctg ccggacctgt cgaaccggct gccggaaggc
     4501 caccgggccg tgacgattat cgtgcaaggc gcagacaccg gcggcaagcg gctgggtgaa
     4561 ggggaccaca tcgacatcgc gttgacggtc gagggctcgc atcccgacct gggcgaagtc
     4621 atgacccgca cgctgatgca caacgtcctg atcgtcgatg ccgctgccag ccgcccgctg
     4681 atgcggggca cgcggcggac caccgacctg aatggctcgt cgatcaccgt cgcggtcacg
     4741 cccgttgacg ccaacaagct gatcgtcgcc cagcggaccg gcatcttgca ggccacgctg
     4801 gtcagcgcca aggacaacgc cccgattggc gccagcgccg atccggtcag ccgccggcag
     4861 ctgctcggcc tgaaagaaat cgtgccgccg aagaagtaca cggtggaaaa gtggagcggc
     4921 aacagcgtcc agatcctcga gatgagcaac gaccggattc gcgaatcgcg cgaaatctcg
     4981 gccggccgcc gcgaggtgcc cgtctcgcag ccgattcccg ccccggccaa cgaccaggtc
     5041 ggccgtattc ccacgggggt gaaggtgccc gacgttgcgt atgagctggg cgaggagccg
     5101 ctggctgccc tggccccggt cgccccggag ggcgcgaagg aagtgaagta agttagggcc
     5161 gtcatacgtt cgccgctcct ctggcagcgg cgaatccatc gcgccaattc cagcgaccaa
     5221 tagcaccagc gaagtcaagg caagggagca ccgaacgatg tttgcaaacg cctttcggcc
     5281 ggccggagaa cgcaacggca acggcaatgg caatggccac ggccatgccg agaccctgat
     5341 gcaggcggag aacctggaag ccgaattcca gcgcctcaag accgggatcc accgcgagct
     5401 gctcgattcg ctcgacctct cgcggatcac cggcctctcg gatgaagagc tccgccacga
     5461 catccgccag ctcgccgaag gcatgctccg gacccgttcg cggaagctgc cggcgatcga
     5521 cgaggagcga ctgatcgagg agctgatcgc cgaatcgttc ggcctcggcc ccctggaacc
     5581 ctacatgcag gacccggacg tcagcgacat tctggtcaat ggcccgcacg aggtctatgt
     5641 cgaacggctc ggccgcctgc aggagacgaa caccgtcttc gccgacgacg agcacctgct
     5701 gcaaattatc cagcggatcg tggcccgcat cggccgccgc atcgacgaac atcagccgat
     5761 ggtcgatgcc cgcctgcccg acggcagccg cgtgaacgcg atcatcccgc cgctggccct
     5821 ccgcgggccg gtgctgtcga ttcgccgctt cggccaccgg ccgctgcaga tcgccgacct
     5881 cttggtccgc ggctcggtct gcccggaaat gctcaagctg ttcgagatgg cgatcgaagg
     5941 ccgcatcaac gtgatgatca gcggcggcac cggcagcggc aaaacgacgc tgctcaacaa
     6001 cctctcgaaa ttcatcccgg ccaacgagcg gctggtgacg atcgaagact cggccgaatt
     6061 gcgattgcag cggaagcacg tggtcaagct cgagacgcgg cccaagagcg ccgaaggcac
     6121 ggccgaggtt tcgcagcgcg acctggtccg caacgccctc cgcatgcgtc ccgaccgaat
     6181 catcctgggc gaagtccgcg gaggcgaagc cctcgacatg ctccaggcca tgaacacggg
     6241 ccacgaaggc tcgctgacga cgatccacgc caacgacacg cgcgacgccc tgtcgcgcct
     6301 cgaagtaatg gtcagcatgg cgggcttcga tttgccggtc ggcgtcacgc ggcggtacat
     6361 cgcgtcggcg atcacgctgg tcatccacct ggctcgtctc aagggcggcg tccgccgcgt
     6421 catgcgggtc tcggaaatca cggccctgga aagcggcgac tacgccattc aggatttgtt
     6481 cggcttccag cagaccggcg tcgatgaccg cggcatcgcc cgcggacact tcttcgccac
     6541 cggacacatg ccggtcttcg cccgccgctt gatcgagcaa ggcatcgacc tgcccacgaa
     6601 gctgttcgag ccccgccagc tcacacccga cagttccatc ggcccgatga gcgtcaccgc
     6661 cgccggcggc gcggcccccc tggaggaaat ggcatgatga ttttcgccaa cctggcggtg
     6721 gtcggcgtca cgttcgcttc ggtgacgatg atcctggccg ccgtcgggat gttcattcgc
     6781 gatctgttcg cgggaagcgc ttccacggcg cggcagcggc tgaccttcgc ccctgaagag
     6841 cccgccggcg acatcaaccg ctcgttcttc cacctggtgg aagaatcagg cacgacgatg
     6901 gacatgtcca cggccctggc gatggtgatc ggggccggga tcgtgggggt caccattccg
     6961 ctggccctgt tcgacaacct gctcgcggcg gccggcggcc tggtgctcgg cgcggcgatt
     7021 ccgattctgt atctgtcggt cgtgcgctgg tggcggctgg gcggcatgcg gaagaagctc
     7081 ccgtacgccc tgcaagcggt ggcc
//
LOCUS       DASRJB010000584         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_168055, whole genome shotgun sequence.
ACCESSION   DASRJB010000584 DASRJB010000000
VERSION     DASRJB010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168055"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFB80_15420"
     CDS             <1..384
                     /locus_tag="VFB80_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN35217.1"
                     /translation="DPAWKLDGVNLLPYLTGQNKAKPHETLYWRFGEQWAIRHGDWKL
                     VVGNEPGSPATKEKPAELINLAADIGEKQNLAAEQPAKVKELQGLYAAWNAEQKDPLW
                     RPNPAAKKAKQAKKAAKAAKAAAGK"
     gene            complement(503..1234)
                     /locus_tag="VFB80_15425"
     CDS             complement(503..1234)
                     /locus_tag="VFB80_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HZN35218.1"
                     /translation="MLLSLVLSASAVLAAEGQAPAAGGSAPARKTLKVARTDDFEITG
                     DGSAAAWSKAAWEPLVPRGTQPREDKTQVKALHSPKGLYFLMQGADRAVTAKMNEDFA
                     DLWKEDVFEVFLWPDEKTPLYFEYEISPLGYELPILVPNFQGKFFGWRPWHYEGDRKI
                     KKATSVQGGSKTSGSPCTGWTAEFFIPYALFTPLENVPPKPGTTWRANFYRMDYDGGQ
                     TSSWDWSRVGPSFHDIQNFGLLVFE"
     gene            1799..>4402
                     /locus_tag="VFB80_15430"
     CDS             1799..>4402
                     /locus_tag="VFB80_15430"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN35219.1"
                     /translation="MRNWTLFPVALLAMLASPLAAAPPAAADHDRRFAETVVPFLQTY
                     CQSCHGKTKQEAKLDLSAFTTAASVTQRHQTWALVLERLEAGEMPPDEAKQRPTTEER
                     KATVAWIRDFRKLEAERRAGDPGVVLTRRLNNAEYNYTIRDLTGVDLRPTREFPVDPA
                     SEAGFDNSGESLAMSPALLGKYVAAAREVADHLVLTPRGLAFAPHPVVTDTDRDKYCV
                     QRIVDFYLRQPTDLAPYFLAAWKYQHRAALGQPEAKLADIAALEKISPQYLATVWTAL
                     TGADAEVGPMARVQSMWRELPAPVEKDSAAAAEGCQRIRDFVKQLRRKLEPRLSDLKV
                     RGIHEGSQCFVLWKNREYASYRRRYDASNLQIEGEPLGKREADPDLAVPADPAARKRF
                     EDSCAQFCDVFPDAFYISERGRDYTGKPKEQQEKGRLLSAGFHSMMGYFRDDQPLCDM
                     ILSESQREELDELWRELDFVASAPMRQYTGFLWFERTDSRYMRDPEFDFARSENKDVT
                     GEAMIQRLAEVYRTKAINSGGAGTPIQAIDDFFRNINAQIRWVEQARLAAEPIHLAEL
                     LRFAERAYRRPLAAAEREDLLNFYRTQRSEASHEEALQDALVSVLMSPHYLFRMDLAR
                     QGEAARRLSDVELASRLSYFLWSSLPDEELMQRAAAGDLHNPDVLLAQTRRLLRDDRV
                     RGLALEFGGNWLDFRRFESHNSVDRDRFPQFTSELRQAMFEEPIRFFLDNVQHDRSVL
                     DFLYGKHTFVNPLLAEHYGISGVSGGGDDWVRIDDAASYGRGGLLPMAVFLTQNAPGL
                     RTSPVKRGYWVVRRLLGEQIPPPPPTVPELPKDEAKLGELTLPQVLARHRDDKACAGC
                     HRRFDSVGLVFE"
BASE COUNT          823 a         1424 c         1409 g          746 t
ORIGIN      
        1 gatccggcct ggaagctcga cggcgtcaac ctgctgccgt atttgacggg ccagaacaag
       61 gccaagccgc acgagacgct ctactggcgg ttcggcgagc agtgggccat ccgccacggc
      121 gactggaagc tcgttgtcgg caatgagcca ggcagccccg cgacgaagga gaagcccgcg
      181 gagctgatca acctggcggc cgacatcggc gagaagcaga acctggcggc tgagcagccg
      241 gcgaaggtca aagagctgca aggcctctac gccgcctgga acgccgagca gaaggacccg
      301 ctctggcgtc cgaacccggc ggccaagaaa gccaagcagg cgaagaaggc ggctaaagcg
      361 gcgaaggctg ctgccggaaa gtagtcgccg tggtgcagga cggagagagg gagagaatga
      421 gagacggagg acggagatgc cctctatccc tctgtccctc cgtctctctg tccctccgtc
      481 tctctgtcac tccctcgcgg cgttactcaa acacgagtag gccaaaattc tggatgtcgt
      541 gaaaactggg cccgacgcgg gaccagtccc aggaggaagt ctggccgccg tcgtagtcca
      601 tgcgatagaa attggcccgc caggttgtgc ccggcttggg cggcacattc tccagcggcg
      661 tgaagagcgc gtagggaatg aagaactcgg ccgtccaccc ggtgcacggg ctgccgctgg
      721 tcttgctgcc gccctgcacg ctggtggcct tcttgatctt gcggtcccct tcgtagtgcc
      781 agggccgcca gccgaaaaac ttgccctgaa agttcggcac caaaatcggt agctcatagc
      841 ccaacggcga aatctcgtac tcgaaataga gcggcgtctt ttcatccggc caaaggaaga
      901 cctcgaacac gtcctccttc cacaggtcgg caaagtcttc gttcatcttg gccgtcaccg
      961 cccggtcggc cccctgcatc aggaagtaca gccccttcgg cgaatgcagc gccttgacct
     1021 gcgtcttgtc ctcccgcggc tgcgtccccc gcggcaccag cggctcccag gccgccttgc
     1081 tccaggccgc cgccgagccg tcgcccgtaa tctcgaaatc gtcggtgcgg gcgaccttga
     1141 gcgtcttgcg ggccggtgcg gagcctccgg cggcaggggc ctgcccttcg gctgccagca
     1201 cggcggacgc ggaaagaacc agggacagca gcatggcgag ggctccgagg agggtgacgc
     1261 gcatcatacg gcacggcagg gacgcgtgaa ccagggccct ctttttccag cctcctcgct
     1321 gtctggacaa acacacacgc ctttcgtata atatgtccac taaatgcctt ggatttgagg
     1381 cgcgcgcgac tttcgcgttt tcgccctttc ggtgtttcgc gttccgccgg ctgttcggta
     1441 cgcgcataat gcgcataacc gcggaaccgc gtttttggac acaagtcgat ggctggcaat
     1501 aacttgcgaa agattgcaac ctccagaaag aaattggcgg ttatgcgcgc atggcaaacc
     1561 gttggacagg cagatcgcga aagcgcgaaa gtgcgacagc gcgaatgagt gaatcagagt
     1621 tggtgcgcgc ataatgcgca tagtccctga gaggcgattt gcgccgcaag tccatacgga
     1681 ataagaactt gcggcagaca atgacgcccg gagaagattt ggcgatatgc gcgcatggac
     1741 ggatgccggc cggtccgttc aactagaatc gacgctcgcg gaggccaaat tccgagccat
     1801 gcgaaactgg acgctctttc ccgtagccct gttggcgatg ctggccagtc cgctcgcggc
     1861 tgccccgccc gccgcagcag accacgaccg gcgatttgcc gaaacggttg tcccgttttt
     1921 gcagacgtac tgccaatcct gtcatggcaa gacaaagcag gaggcgaagc tcgatctgtc
     1981 ggcgtttacg accgccgcca gcgtcaccca gcgacatcag acttgggcac tggtcctgga
     2041 gcggctggaa gcgggcgaga tgcctcccga cgaggccaag cagcggccga ccacggaaga
     2101 gcggaaggcg accgtcgcct ggattcgcga ctttcgcaag ctcgaggcgg agcgccgggc
     2161 gggcgacccg ggcgtcgtcc tcacccgccg gctgaataac gcggaataca actacacgat
     2221 ccgcgatctg acgggcgtcg atctccgtcc gacccgcgaa ttccccgtcg atccggccag
     2281 cgaagcgggc ttcgacaatt cgggcgagtc gctggccatg tcgccggccc tgttgggcaa
     2341 atacgtcgcc gcggcccgcg aggtcgccga tcatctcgtg ctcacgccac gtggactggc
     2401 gttcgcgccg catccggtcg tcaccgatac cgaccgcgac aagtattgcg ttcagcggat
     2461 cgtcgatttc tacctgcggc agccgacgga cctcgcccca tacttcctgg cggcctggaa
     2521 gtatcagcac cgtgcggcgc tcgggcagcc ggaagcgaag ctggccgata tcgccgccct
     2581 ggagaagatc agcccgcagt atttggcgac cgtctggacg gcgttgaccg gcgctgacgc
     2641 cgaagtcggg ccgatggcga gagtgcaatc gatgtggcgc gagctgcccg cgccggttga
     2701 aaaggactcc gctgctgctg ccgaaggctg ccagcggatc cgcgacttcg tgaagcagct
     2761 ccgccgcaag ctggagccgc ggctatctga cctgaaggtg cgcggcatcc acgaaggctc
     2821 gcaatgcttc gtgctctgga agaaccgcga gtacgcgtcg taccgccgcc gctacgacgc
     2881 cagcaacctg cagatcgaag gcgagccgct aggcaagcgc gaggccgatc cggatctggc
     2941 cgtgcccgcc gatcccgccg cgcggaagcg ttttgaagac tcctgcgcgc aattctgcga
     3001 cgtcttcccc gacgcgttct acatttccga gcgcggccgc gactacaccg gcaaacccaa
     3061 ggagcagcag gaaaaaggcc gccttctctc ggccggcttc cacagcatga tgggctattt
     3121 ccgcgacgat cagccgctgt gcgacatgat cctcagcgag tcgcagcggg aggagctcga
     3181 tgaactgtgg cgggagctgg actttgtggc ctcggcgccg atgcggcaat acacgggctt
     3241 cctttggttc gagcggaccg actcgcgcta tatgcgcgac ccggaattcg acttcgcccg
     3301 ttcggagaac aaggatgtta ccggcgaggc gatgatccag cggctggccg aagtctatcg
     3361 caccaaggcg atcaacagcg gcggtgcggg cacgccgatc caggcgatcg acgatttttt
     3421 ccgcaatatc aacgctcaga ttcgctgggt cgaacaggcc cggctggctg ctgagccgat
     3481 ccacctggcg gagctgctgc gcttcgccga gcgggcctat cgccggccgc tggcggccgc
     3541 ggagcgcgaa gacctgctga acttctaccg cacgcagcgg tccgaggcga gtcacgaaga
     3601 agccctgcag gacgcgctgg tctcggtgct catgtcgccg cactatttgt tccggatgga
     3661 cctggcccgg cagggcgaag ccgcccggag attgagcgat gtcgagctgg ccagccggct
     3721 gagctatttc ctctggtcga gcctgcccga cgaggaactg atgcagcggg ccgccgcggg
     3781 cgacttgcac aatccggacg tgctgctggc gcagacgcgc cgtctgctgc gcgatgaccg
     3841 cgtccgcggg ctggccctgg agtttggcgg caactggctc gacttccgtc gcttcgagtc
     3901 gcacaacagc gtcgatcgcg accgcttccc gcaattcacg agcgagctgc ggcaggcgat
     3961 gttcgaagag ccgatccgct tcttcttgga caacgtccag cacgatcgct cggtgctcga
     4021 tttcctgtac ggcaagcaca cgttcgtcaa tccgctgctc gccgagcact acggcatttc
     4081 cggcgtcagc ggaggcgggg acgactgggt gcgaatcgac gatgccgcca gctacggccg
     4141 cgggggcctc ttgccaatgg ccgtcttcct gacgcagaac gcgccaggcc tgcgcaccag
     4201 cccggtcaag cgcggctact gggtcgtccg tcggctgctc ggcgaacaga ttccgccccc
     4261 tcctcctacc gttcccgaac taccgaagga cgaggcgaag ctgggagagc tgacactgcc
     4321 gcaagtgctg gcgcggcacc gcgacgacaa ggcttgcgcc ggctgccacc gccgcttcga
     4381 ttcggtcggc ctggtcttcg ag
//
LOCUS       DASRJB010000585        13062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1579986, whole genome shotgun sequence.
ACCESSION   DASRJB010000585 DASRJB010000000
VERSION     DASRJB010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579986"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFB80_15435"
     CDS             <1..288
                     /locus_tag="VFB80_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35220.1"
                     /translation="LDACQIENLWYFDGLPDTAEKAGRSFAAVPERHLFVGHMHCWLV
                     ITPTGRVDWIGETPLALRREPRYLVAVAAVVDGWCAIYDTAPEELTPVRCD"
     gene            complement(292..945)
                     /locus_tag="VFB80_15440"
     CDS             complement(292..945)
                     /locus_tag="VFB80_15440"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN35221.1"
                     /translation="MILLLDNAVRIPPAAAQDFAAFRRWTKSKDFPQRGEYAFLGGDL
                     WAGISMETLVHNQIKLRIAAVLSLMVVDPLKLGRVFSDRMRLVYEPVHLSCEPDAMFV
                     SHASVDAGRVRWEEGRESLEVIGTPDMVLAVVSSNSIEKDTVVLRDLYAQTGIPEYWL
                     VNPLEGQLSFDILRRSGRRYVATRKSAGWVKSAVFGKSFRLVEESGDDAPQYQLLVR"
     gene            complement(1063..1749)
                     /locus_tag="VFB80_15445"
     CDS             complement(1063..1749)
                     /locus_tag="VFB80_15445"
                     /inference="COORDINATES: protein motif:HMM:NF042733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6690 family protein"
                     /protein_id="HZN35222.1"
                     /translation="MFQRPVLLVSLLAAAVGVPYLLLDKNLSETARGQWRRLSGSLSA
                     TPAATSENASKSGIETPAALTVPQVSIEEAFRFDVRPEWVASRFKSVSTVTDSKQLGM
                     RVALVSGTRPDDVAGSLTYYFDEYHQPQRITFTGQSGDARRLLAAVVGPYGLKSLPTT
                     DAARYVAGNPKQPTSQVVVKYLPQLQGSPTSPRNEIALDLRRTDAIGWEQRQVQAGEA
                     SLLPPSYRQW"
     gene            complement(1847..2269)
                     /locus_tag="VFB80_15450"
     CDS             complement(1847..2269)
                     /locus_tag="VFB80_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35223.1"
                     /translation="MTPPIELLGSYLHLARAAELRRQPLVRDRVLLLAAVIAAQIDLV
                     PIAAACRERILSHNARHVIGRWPTVAEALAEEDFQTLVVQLSGRYGPERVERLVAQLG
                     IERGRERDAYASDGEYAAGLLGLDWEDLQRRHPAEPDE"
     gene            2338..3204
                     /gene="folP"
                     /locus_tag="VFB80_15455"
     CDS             2338..3204
                     /gene="folP"
                     /locus_tag="VFB80_15455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN35224.1"
                     /translation="MAMNSQSNSGRANSWKLRTRTLACGERPLLMGIVNVTPDSFSDG
                     GKFLDHQAAVDHAIQLLDDGADIVDIGGESTRPYSTPVAEEEELRRVLPVIEDVFRQR
                     PAVVISIDTSKPAVAEAAIAAGAEIINDITGLNDVRLAHVARESGAGVCVMHMQGTPQ
                     TMQDNPTYGDVVAEIHVELRQRRDALVAAGIERGRICLDPGIGFGKTHEHNIELMANC
                     WRFHDLGCPLLVGHSRKGFLGKLIGDKAADRTAATIGGALALAAQGVQILRVHDVRPL
                     REALAVFAAASP"
     gene            3567..5747
                     /locus_tag="VFB80_15460"
     CDS             3567..5747
                     /locus_tag="VFB80_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HZN35225.1"
                     /translation="MSHSAPRFQAIHAVTNYKPSAPPLNFKELSSHEVFGANVFSMNE
                     MAKRLPKPVFKSLKKTIETGAKLDPTIADIVATAMKEWAIEKGATHYAHVFYPLTGLT
                     AEKHDSFLAPTADGRAVAEFTGAQLAQGEPDASSFPSGGIRATFEARGYTAWDVTSPA
                     YILENANGTTLCIPTAFVSWTGEALDKKTPLLRSMKALNVQAQRILKLLGHKDVGHVS
                     ATAGPEQEYFMIDRNFFFARPDLLNAGRTLFGAKPPKGQEFEDQYFGAIRDRVLACML
                     EAERELFKLGIPVKTRHNEVAPNQYELAPIYENANIATDHQQLIMLTLKKVAQKHGME
                     CLLHEKPFAGINGSGKHLNWSLGNTTQGNLLEPGDTPHENMQFLIFCAAVIRAVHKFS
                     GLLRAVVATANNDHRLGANEAPPAIISIFLGDQLTDVFEQIKAGGAKTSKAKGTLTVG
                     VDTLPVLPKDAGDRNRTSPFAFTGNKFEFRAVGSSQSLAGPLVALNTIVAESLDYIAT
                     ELERTTGGDPGKLNSAVQKVLQDITAKHSAVIFNGDNYTAAWHEEAEKRGLPNLKTCV
                     DAIPVLASPEVVQLLDKYGVLSPRETQSRHDIYFEQYVKSISTEAKLIIEMARTMIFP
                     AAVRYQNELATTCTNLKALGYTFDTDTLDEITRLVKGLQDSTTALEKSLAHHGGGLID
                     EAKHFCKSVLPAMAKVRDFADRLEGYVADDLWPLPTYQEMLFIK"
     gene            5863..7275
                     /gene="glnA"
                     /locus_tag="VFB80_15465"
     CDS             5863..7275
                     /gene="glnA"
                     /locus_tag="VFB80_15465"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZN35226.1"
                     /translation="MSPREVLALCREKDVKAVDLRFCDLLGTWQHFTIPVGQLDEGVF
                     DDGLGFDGSSIRGWQGIHESDMLVLPQPDTALLDPFAALPTLAMIATVQDPVTGEPYS
                     RDPRYIAIKAANFLRQTGIADVAYFGPEAEFFVFDSARFESSAHEAYYHLDSEEGAWS
                     RGREGAGAHGYKLRHKEGYFPCPPADQLMDLRSEMMQAMLDCGLDVEAHHHEVASGGQ
                     CEIDLKYQPLVTMADWVQIYKYCVRNVARRRGKTATFMPKPLFGDNGSGMHVHFSFWK
                     NDEPLFAGGSYAGLSEIGLYAVGGILRHAPALLALTSPTTNSYKRLVPGYEAPVNLVY
                     SQRNRSAACRIPMYSQSPPSKRIEFRCPDPTCNPYLAFAAIAMAAIDGIQLKLSPGQP
                     VDKDVYHLPPEELAEIRKTPATLDEALTALEKDCEFLLKGDVFTSDVIETWISYKRTN
                     EVDAVRLRPHPYEFCLYYDA"
     gene            7369..8097
                     /locus_tag="VFB80_15470"
     CDS             7369..8097
                     /locus_tag="VFB80_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZN35227.1"
                     /translation="MAITKELLTRLARFDTPTICNVIELFDIRPRNRGYMDGRIKCNF
                     PELPNMVGFACTAAFRSDAPPAGGDAYGSIGQQLEQFAKLPGPAVVVFQDLDDPPAAA
                     VFGEVMCSSYQAFGSAGLVTSGAGRDLEQVRALKYPVFTSGTICSHGYCHMLHLGLPV
                     RVGGLMVNQGDLLHGDANGVTNIPIEIAAEVADIGGEFLAAEELVMSYVKGGGDKTAS
                     RYNELRKEFQSIVAALTKRVSRKK"
     gene            8184..8726
                     /gene="hslV"
                     /locus_tag="VFB80_15475"
     CDS             8184..8726
                     /gene="hslV"
                     /locus_tag="VFB80_15475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZN35228.1"
                     /translation="MKFRSTTILTVRHKGTLALGGDGQVTLGTTVMKADAAKIRRLRD
                     GNVLCGFAGSSADAFALLERFEARLKDNPGNVPRAATELAKEWRTDRALRRLEALMVV
                     ADVRHSLLISGTGDVIQPADGIIGIGSGGPLAVAAARALVAHSSLKAAEVVREALKIA
                     GELDIYTNQNIVVEELPCEP"
     gene            8714..10084
                     /gene="hslU"
                     /locus_tag="VFB80_15480"
     CDS             8714..10084
                     /gene="hslU"
                     /locus_tag="VFB80_15480"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZN35229.1"
                     /translation="MRALASKADDAGVQIRNLTPREIVAELDRYIVGQHDAKRAVAIA
                     IRNRWRRQQLPDELRQEVSPKNILMIGPTGVGKTEIARRLAKLTGAPFIKVEATKYTE
                     VGYYGRDVESIVRELLDSAISLVRQRERTKVEDEARVAVEERLLDLLTQRERESAERE
                     PAAETSREELTERLRKRLLAGELEDRKVELLIEARATPIMVGGVGMEHLDIDLQGMFE
                     KILPKQTNRREVSVAQARQILFEQECEGRLDQDKIHATSIELAENLGIIFIDEVDKIV
                     ASEGRGADVSRQGVQRDLLPIVEGTQVQTRYGYVRTEHILFIAAGAFHKSKPSDLMPE
                     LQGRFPIRVELKDLTKDDFVRILTEPKNSLTRQYAALLETEGVTLDFNQSAIDALAEF
                     AYRVNQTTQNIGARRLYTILERLVEELSFEAPDMKMGKVEITAKYVQERLTQIAEDED
                     LSRYIL"
     gene            10240..11568
                     /locus_tag="VFB80_15485"
     CDS             10240..11568
                     /locus_tag="VFB80_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN35230.1"
                     /translation="MSCRGQALDAAGKPIAGATVFLVSTNQVDKPLGNRTTDADGKYE
                     FREVEIPIPPRREGAAEEDYSAATFQVFGKASGHGLAWRGMKWLYIGRSYFGPDGELL
                     PQNHENGFPLGRDIELNLTFPAAYGIAGRFVDEAGKPVAGVKIRLGNCDYLDPAGKES
                     HRNFREFWALHLAADLMPDAVTAKTDAEGRFNLAAAPPETVCWLLIEHPDFAKISLYT
                     ATTAAPPTDHDKHPVHKLPLEISLRAAKSIPLSVVWADTGKPAAGVRVHASQMRASGS
                     ASYGTTDAAGKFVLKLPSGTYKLTGDPPRDSDYVRTTVELKVKEAPAEQPASLAIDRG
                     AVLLFKATDFDTGAPIARVSFWYEPEDRPGSRFGVQPNPSIVTHPKTNEKGEMRVVAA
                     PGTRKYGVGFNPLPAGYEPSVATDSTPGRELVLSAGEERVVEFLLRKREK"
     gene            complement(11719..12444)
                     /locus_tag="VFB80_15490"
     CDS             complement(11719..12444)
                     /locus_tag="VFB80_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35231.1"
                     /translation="MDTRELSSPTTRLGIIHLLGWMLGCGAVLAIYRLNMPATPTLPD
                     LLRHLGLGLAYGTAASGLGLFLWRWRTGSGPAPTQPGHWLLIFGGLGMLIDMGTTAPY
                     LLALKLGVREAFVWWLCQQLAAWSIATLIGLVVLTRLRGASRTWTTAAWIIVFALGLN
                     AAADITSLIGIRFSAGTWTWYLPIGAHLASVALALPPVWGAELADRLRGVPRDWLHYT
                     GIASVSILAVVDFTIHYIALRSS"
     gene            12536..>13062
                     /gene="eno"
                     /locus_tag="VFB80_15495"
     CDS             12536..>13062
                     /gene="eno"
                     /locus_tag="VFB80_15495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123706.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZN35232.1"
                     /translation="MSLIASVHARQILDSRGNPTVEVEVTLSDGSFGRAAVPSGASTG
                     MHEAWELRDGDKAVYMGKGVTKAVHNANTIIADSLTGWDALDQIGVDQRLIELDATPN
                     KKNLGANAILGVSLAVAKAAASFTQQPLYRYLGGVGANLLPAPMMNIINGGAHADNSI
                     DLQEFMVMPLGFQRF"
BASE COUNT         2509 a         4227 c         4181 g         2145 t
ORIGIN      
        1 cttgatgcgt gccagatcga aaacctgtgg tacttcgacg gccttcccga cacagcggaa
       61 aaagcgggcc gcagcttcgc cgccgtgccc gagcggcatc tcttcgttgg acacatgcat
      121 tgctggctgg tgataacgcc caccggccgg gtggactgga tcggcgaaac gccgctggca
      181 cttcgccgcg agccgcgcta cctcgtggct gttgcggcgg tcgtcgatgg gtggtgtgcg
      241 atctacgata cggcccctga ggaattgacg ccggtgcgct gcgattagcc gtcaacgcac
      301 gagcaactga tattgcggtg cgtcgtcgcc tgattcttcg accaggcgaa atgacttgcc
      361 aaagacggcc gatttgaccc agccggcact cttgcgcgta gcgacatagc ggcggcccga
      421 gcggcggagg atgtcgaacg agagctggcc ttcgagagga ttgaccagcc agtattctgg
      481 gatgccggtt tgggcataca ggtcgcgcag gaccacggtg tccttttcga tcgagttact
      541 gctgacaact gcgagaacca tatccggcgt gccgatgact tccagcgact cgcggccttc
      601 ttcccagcgg acgcgcccgg cgtctaccga tgcgtggctg acgaacatag cgtccggttc
      661 acacgacaag tgaaccggct cgtacaccag ccgcatgcgg tcactgaaaa ctcgccccaa
      721 cttgagcgga tcgacgacca tcaacgaaag gacggctgca attctcagct tgatctgatt
      781 gtggacgagc gtttccatcg agattcccgc ccacaagtcg ccgcccagga aggcgtattc
      841 gccgcgctgg gggaagtcct tcgacttcgt ccaacggcga aacgccgcga agtcttgggc
      901 cgctgcgggc ggaatgcgga ccgcgttgtc caggagcaga atcattggcg agcagacctg
      961 ttgaggatca tcctaccgtc ggggattcct gccgagaggc ttcgtccggc acagcccact
     1021 cgcaccgccg gggtacgact tcacgtggag cgtgaggact actcaccatt gccgatacga
     1081 cggcggcagg aggctggctt cgccggcttg aacttggcgc tgctcccagc cgatcgcgtc
     1141 ggtgcgccgc aggtcgaggg cgatctcgtt ccgcggactg gtcggcgagc cttgcagctg
     1201 cggcaggtat ttgacgacga cctggctcgt cggctgcttg ggattgccgg ccacgtagcg
     1261 ggcggcatcg gtggtgggca gcgacttcag gccgtaaggg ccgaccaccg cggccaagag
     1321 gcggcgggcg tcgccgctct gacccgtgaa cgtgattcgc tgcggctgat ggtattcgtc
     1381 gaagtagtag gtcagcgagc cggcgacgtc gtcgggccgc gtgccgctga ccagggccac
     1441 gcgcatgccg agctgcttcg aatcggtcac ggtgctcacg ctcttaaacc ggctggcgac
     1501 ccactcgggc cggacgtcaa agcggaaggc ctcctcgatg ctcacctgcg gaaccgtcag
     1561 cgccgcgggc gtttcgatgc ccgacttgga ggcattttcg ctcgtcgccg ccggcgtggc
     1621 agaaaggctg ccgctgaggc gccgccactg tccgcgggcc gtctcggaga ggttcttgtc
     1681 gagcagcaga tagggaacgc cgactgccgc tgccagaagc gaaaccaaga gcaccggacg
     1741 ctgaaacatg cgtgccgctc cgatcgcgag agaatctgcc acttcgcagg atcggcgctg
     1801 gcgatctgcc gacttgtgga atcgggccgc ccgccgcacg ccgcgactat tcgtcaggtt
     1861 cggcaggatg ccgccgctgc agatcttccc agtcgaggcc gagcaaaccc gccgcatact
     1921 cgccgtcgct ggcataggcg tcacgctcgc ggccccgctc gatgcccagt tgcgcgacca
     1981 gccgctcgac ccgctccggg ccgtagcggc cggaaagctg cacgaccaac gtctgaaaat
     2041 cctcttccgc cagggcttca gccaccgtgg gccagcggcc aatcacgtgg cgggcattgt
     2101 ggctcaaaat ccgctcgcgg caggcggcgg caatcggcac gaggtcgatt tgcgccgcga
     2161 tcacggcggc cagcagcagc acgcggtccc gaacgagcgg ctgccggcgc aactctgcgg
     2221 cgcgggccag gtgcaagtaa ctgccgagca gctcgatggg cggcgtcatg tgcccatcat
     2281 ggcagccggc ggcggccgtt cccagcagat gtttggccca gcggctagga tgacggcatg
     2341 gccatgaatt cacaaagcaa ttcggggcga gcgaacagct ggaagctccg cacgcgaact
     2401 ctagcgtgcg gcgagcggcc gctcttgatg ggcatcgtga acgtcacgcc ggacagtttt
     2461 tccgacggcg gcaagttcct ggatcatcag gcggccgttg accatgcgat ccaactgctc
     2521 gacgatggag cggacatcgt cgatatcggc ggcgaaagca cacggccgta ttcgacgccc
     2581 gttgccgagg aagaagagct gcgccgcgtg ctgccggtaa ttgaagatgt gtttcggcag
     2641 cggccggcag tcgtgatctc gatcgatact tccaagccgg ccgtcgccga ggcggcgatt
     2701 gccgccggcg cggagattat caacgacatc acgggcctga acgatgtgcg gctcgcccac
     2761 gtcgccaggg agagcggggc gggcgtgtgc gtgatgcaca tgcaaggcac tccgcagacg
     2821 atgcaggaca acccgaccta tggggatgtc gtggccgaga ttcatgttga gctgcggcag
     2881 aggcgcgacg cactcgtggc cgcgggcatc gaacgcgggc gaatttgcct cgacccgggc
     2941 atcggcttcg gcaagactca cgaacacaac atcgagctga tggcgaattg ctggcggttt
     3001 cacgacctgg gctgcccgct gctggtcggc cactcgcgaa agggatttct gggcaagctg
     3061 atcggcgaca aggccgccga tcggacggcc gcgacgatcg gcggggcact tgccctggcc
     3121 gcgcaaggcg tgcaaatcct tcgcgtgcat gatgtccggc cactgcgcga ggccctggct
     3181 gtgtttgccg cggcgagtcc ctgaaaaacg ccactgggtg cttggcaggc cagcgcccag
     3241 tctttaaaac atggacgccc attatggagg agtaggcgcg cggggaagaa ttaggatcga
     3301 taattgtgca cggagtttat ggcctttcgc cgcgcttttg ggcacggctg cccgcaatgc
     3361 atgcatgacg ctagcatgag aagggggagc ctcaccagct gctacgacct aagtctttga
     3421 taacacatta cttaggaatc ccagctcgat tcatcggtat ccattgacgt tggcacgttg
     3481 cgtgcttcac aaagggcgat ttgagagcta cggctctcgg gcgtatttca gacatcgggc
     3541 atctttaggg taggagtgtt gttgccgtga gtcacagtgc acctcgtttc caggccatcc
     3601 acgccgtcac caattacaag ccgagcgcgc cgccgctcaa cttcaaagag ctgtcgtcgc
     3661 acgaagtctt tggggcgaac gtcttctcga tgaacgagat ggctaagcgg ctgccgaagc
     3721 cggtcttcaa gtcgctgaag aagaccatcg aaacaggggc caagctcgac ccaacgatcg
     3781 ccgatatcgt ggcgacggcg atgaaggaat gggcgatcga aaagggcgcc acgcactacg
     3841 cccacgtgtt ctacccgctc accggcctga cagccgagaa gcacgacagt ttcctcgcgc
     3901 cgaccgccga cggccgggcc gttgccgagt tcaccggcgc gcaactcgct caaggcgagc
     3961 cggatgcttc gagcttcccg tcgggcggca tccgggcgac gttcgaagcc cgcggctaca
     4021 cggcttggga tgtgaccagc ccggcctaca tcctggaaaa cgccaacggc acgacgctct
     4081 gcattcccac ggcgttcgtg agctggacgg gcgaggccct cgacaagaag acgccgctcc
     4141 tcaggtcgat gaaggccctc aacgtgcagg cccagcgaat tctgaagctg ctggggcaca
     4201 aggacgtggg gcatgtctcg gcgaccgccg gtcccgagca ggaatacttc atgatcgacc
     4261 ggaacttctt tttcgcccgg ccggacctgc tcaacgcggg ccgcacgctg ttcggcgcga
     4321 agccgcccaa ggggcaagag ttcgaggacc agtactttgg cgccattcgc gaccgcgtgc
     4381 tggcctgcat gctcgaagct gagcgcgagc tgtttaagtt gggcattccg gtcaagacgc
     4441 ggcacaacga ggtcgcgccg aaccagtatg agcttgcccc gatttacgaa aacgccaaca
     4501 tcgccaccga ccatcagcag ctgatcatgc tgacgctgaa gaaggtggcc cagaagcacg
     4561 ggatggagtg cctgctgcac gagaaaccct tcgcgggcat caacggatcg ggcaagcacc
     4621 tcaactggtc gctgggcaac acgacgcagg ggaacctgct cgagccgggc gacacgcccc
     4681 acgagaatat gcagttcctg atcttctgcg cggcggtgat tcgggccgtg cacaagttct
     4741 cgggcctgtt gcgggcggtc gtggcgacgg ccaacaacga ccaccgcctg ggtgcgaacg
     4801 aagccccgcc ggcgatcatc tcgatcttcc tgggcgatca gctcaccgac gtcttcgagc
     4861 aaatcaaggc gggcggcgcg aagaccagca aggccaaggg cacgctgacg gttggcgtcg
     4921 atacactgcc ggtcctgccc aaggacgccg gcgaccgcaa tcgcacgagc ccctttgcct
     4981 tcacgggcaa caagttcgaa ttccgcgccg tgggcagcag ccagtcgctg gccggtccgc
     5041 tggtggccct caacacgatc gtggccgagt cgctcgacta catcgctacg gagctggagc
     5101 ggacgaccgg cggcgatccg ggcaagctca actcggccgt gcagaaggtg ctgcaggaca
     5161 tcaccgccaa gcacagcgcg gtgatcttca atggcgacaa ctacacagcc gcctggcacg
     5221 aagaggccga gaagcgcggg ctgccgaacc tcaagacctg cgtcgatgcc atccccgtac
     5281 tggcttcgcc cgaggtcgtt cagctgctcg acaagtacgg cgtcctctcg ccccgcgaga
     5341 cgcagagccg ccacgacatc tacttcgagc agtacgtcaa gtcaatcagc accgaggcca
     5401 agctgatcat cgagatggcc cgcacgatga tcttccccgc ggcggtgcgg tatcagaacg
     5461 agctggccac gacctgcacc aacctcaaag ccctcggcta caccttcgac accgacacgc
     5521 tcgacgagat cacgcggctg gtgaaggggc tgcaagacag caccaccgcc ctcgaaaagt
     5581 cgctcgccca ccacggcggc ggcctgatcg acgaggccaa gcacttctgc aagtcggtgc
     5641 tgcccgcgat ggccaaggtc cgcgacttcg ccgaccgcct cgaaggctac gtcgccgacg
     5701 acctctggcc gctgccgacg taccaggaga tgctgtttat caagtaggtg agtggtgagt
     5761 ggtaggtgag tgaaaaacga caagcggggg gcgatcatcg cctcccgctt tttgttcgta
     5821 ttgccttctg taccctgtct tctgtttcct gtcgcctcac ccatgtcccc tcgcgaagtc
     5881 cttgccctgt gccgcgaaaa ggatgtcaag gcggtcgatc tgcggttctg cgatttgctg
     5941 ggaacctggc agcacttcac gatcccggtg ggccagctcg atgagggcgt gttcgacgac
     6001 ggcctgggct tcgacggctc cagcatccgc ggctggcagg ggattcacga gagcgacatg
     6061 ctcgtcctgc cgcagcccga tacggcgctg ctcgatccgt tcgcggcctt gccgaccttg
     6121 gcgatgatcg ccaccgtgca ggaccccgtg accggcgagc cttacagccg cgatccgcgg
     6181 tacattgcca tcaaggcggc gaatttcctg cggcagacgg gaatcgccga cgtggcctat
     6241 ttcggcccgg aggccgagtt cttcgtattc gattcggccc gcttcgagtc gtcggcgcac
     6301 gaagcctact atcatctcga cagcgaggaa ggcgcctgga gccgcggccg cgagggcgcc
     6361 ggcgcgcatg gctacaagct gcggcacaag gaaggctatt ttccctgccc gccggccgac
     6421 cagctcatgg acctgcggag cgaaatgatg caggccatgc tcgactgcgg cctggacgtc
     6481 gaggcccacc accacgaagt ggccagcggc gggcagtgcg agatcgacct caagtaccag
     6541 cccctggtga cgatggccga ctgggtgcag atttacaaat actgcgtccg caacgtggcc
     6601 cggcggcggg gcaagacggc gaccttcatg cccaagccgc tgttcggcga caacggcagc
     6661 ggcatgcacg tccacttctc gttctggaag aacgacgagc cgctgtttgc cggcggttcc
     6721 tacgcgggct tatcggagat cggactgtac gccgtgggtg gaatcctgcg gcatgcgccc
     6781 gcgctgttgg cgctgaccag ccccacgacc aacagctaca agcggctggt gcccggctac
     6841 gaagcgccgg tgaacctggt ctattcgcag cggaaccgtt cagcggcctg ccggattccg
     6901 atgtacagcc agagcccgcc gagcaagcgg atcgagtttc gctgccccga tccgacctgc
     6961 aatccgtacc tggctttcgc cgcgatcgcg atggccgcca tcgacggcat ccagctcaag
     7021 ctctcgccgg gccagccggt cgataaagac gtctatcacc tgccgccgga ggaactggcc
     7081 gagattcgca agacgcccgc cacgctcgac gaagccctga ccgcgctcga gaaagactgc
     7141 gaattcctgc tcaaggggga cgtcttcacg tccgacgtca tcgaaacctg gatcagctac
     7201 aaacggacga acgaagtgga cgccgtgcgg ctgcggccgc atccgtacga attctgccta
     7261 tactacgacg cctagacaaa cctcgaagaa ccaaagaacc gaagaaccaa acagtatttg
     7321 gaatgcgtcc ctttggttct ttggatcttc ggttcttgga atttcgcaat ggccatcacc
     7381 aaagaactcc tcacccgcct cgccaggttc gacacgccga caatctgcaa cgtgatcgag
     7441 ttgttcgaca ttcgcccgcg gaatcgcggg tatatggacg ggcggatcaa gtgcaacttt
     7501 cccgagctgc cgaacatggt gggcttcgcc tgcacggcgg cgttccgcag cgacgccccg
     7561 ccggccggcg gcgacgccta cggcagcatc ggccagcagc tcgagcagtt cgccaagctg
     7621 cccgggccgg ccgtcgtcgt gttccaggac ctggacgatc cgccggccgc cgcggtgttc
     7681 ggcgaagtca tgtgctccag ctatcaggcc ttcggctccg ccggcctggt cacgagcggc
     7741 gcaggccggg acctggagca ggtgcgggcc ctcaaatacc cggtcttcac gagcggcacg
     7801 atctgctcgc acggctattg ccacatgctg cacctgggcc tgccggtccg cgtcggcggg
     7861 ctgatggtga accagggcga tctcttgcac ggcgacgcca acggcgtgac gaacatcccg
     7921 atcgagatcg cggcggaagt ggccgacatc ggcggcgagt tcctggcggc ggaagagctg
     7981 gtgatgagct acgtcaaagg gggcggcgac aaaacggcca gccgctacaa cgagctccgc
     8041 aaggaattcc agtcgatcgt cgccgccctc actaagcgcg tctcgcggaa gaagtgacgc
     8101 tctcgtctga cggggtgacc gctaccaccg ggacttgcca agcggtatag aacaaagagc
     8161 gggccactac gacgccagac gccatgaaat tccgctcgac gacgattctc accgtgcgcc
     8221 acaagggtac gctggcccta ggcggcgacg ggcaagtcac gctcggcacg acggtcatga
     8281 aggccgatgc cgccaagatc cgccgcctcc gcgacggcaa cgtgctctgt ggcttcgccg
     8341 gcagtagcgc agatgcgttc gccctgctgg agcggttcga ggcccggctc aaggacaatc
     8401 cgggcaacgt cccgcgggcc gcaaccgagc tcgcgaagga gtggcggacc gaccgggccc
     8461 tgcggcgact ggaggcgctg atggtcgtcg ccgacgtccg gcacagcttg ctcatcagcg
     8521 gtacaggcga cgtgatccag ccggcggacg gcatcatcgg tatcggctcg ggcgggccgc
     8581 tggcagtcgc tgcggcgcgg gccctggtcg cccactcgtc gctgaaggcc gccgaagtgg
     8641 tccgcgaggc tctgaagatc gccggcgagc tcgacatcta cacgaaccag aacatcgtcg
     8701 tggaggaact cccgtgcgag ccctagcctc caaggccgac gatgcgggcg tgcaaatccg
     8761 caatctcacg ccgcgggaga tcgtggccga gctcgatcgc tacatcgtcg gccagcacga
     8821 cgccaagcgg gccgtggcca tcgccatccg caaccgctgg cggcggcagc aattgcccga
     8881 cgagctgcgg caggaagtct cgcccaagaa cattctgatg atcggcccca ccggcgtggg
     8941 caagacggag atcgcccgcc gcctggcaaa actcaccggc gcgccgttca tcaaggtcga
     9001 agccacgaaa tacaccgagg tcggctacta cggccgcgac gtggagagca tcgtccgcga
     9061 gctgctcgat agcgcgatca gcctcgtccg ccagcgcgag cggaccaagg tcgaggacga
     9121 agcccgcgtt gccgtcgaag agcggctgct cgacctgctg actcagcgcg agcgcgagtc
     9181 ggccgagcgc gagccggccg ccgaaaccag ccgcgaggag ctgaccgagc ggttgcgcaa
     9241 gcggctcttg gccggcgaac tcgaggaccg caaggttgag ctgctcatcg aagcgcgggc
     9301 cacgccgatc atggtcggcg gcgtcggcat ggagcacctc gacatcgact tgcagggaat
     9361 gttcgaaaaa atcctgccca agcagaccaa ccgccgcgaa gtctcggtgg cccaagcccg
     9421 gcagattctc ttcgagcagg aatgcgaggg ccggctcgac caggacaaga tccacgcgac
     9481 gagcattgag ctggccgaga atctggggat catcttcatc gacgaggtag acaagatcgt
     9541 cgccagcgaa gggcgcgggg ccgacgtctc gcggcaaggg gtgcagcgcg acctgttgcc
     9601 gattgtcgaa gggacgcagg tccagacccg ctacggctac gtgcggaccg agcacatcct
     9661 gttcatcgcc gcgggggcat ttcacaagag caagcccagc gacctgatgc ccgaactgca
     9721 gggccgcttt ccgatccgcg tcgagctgaa ggacctgacc aaggacgact tcgtgcggat
     9781 tctcacggag cccaagaact cgctcacacg gcagtacgcc gccctgctgg agaccgaagg
     9841 cgtcacgctc gacttcaacc agagcgccat cgacgccctg gccgaattcg cctaccgcgt
     9901 gaaccagacc acgcagaaca tcggcgcccg ccggctctac accatcctgg agcggctcgt
     9961 cgaagaactc tcgttcgaag cccctgacat gaaaatgggc aaggtcgaga tcaccgccaa
    10021 gtacgtccaa gagcggctga cgcagattgc tgaggacgag gacctgagcc ggtatatcct
    10081 gtaggctctg ctcattcaat gcaggcgagg tgcaacatgg ccgcgcgatt gtcaggttta
    10141 gcaagtttcg ccacggcaac tatccttatc ctggcggcgc accaggcggc ggcccaagcg
    10201 ccgcccaccg gccaacaaga gccggctgcg accgtcaaaa tgtcctgccg cgggcaagcg
    10261 ctggatgcgg ccggcaagcc gatcgccggg gcgacagtct ttctggtttc gacgaaccag
    10321 gtcgataagc ccttgggcaa tcgcacgaca gatgccgatg ggaagtatga gtttcgcgag
    10381 gtcgagattc cgatcccgcc gcgccgcgag ggtgcggcgg aggaagacta ttcagcggca
    10441 acatttcagg tgtttggcaa ggcctccggg cacgggctgg cctggcgggg catgaagtgg
    10501 ctgtacatcg gccgcagcta ttttgggccg gatggcgaat tgctgccgca gaaccacgag
    10561 aacgggtttc cgctcggccg cgacatcgag ctgaatttga cgttccccgc tgcctacgga
    10621 atcgcggggc gatttgtcga tgaggcgggc aagccggtgg cgggggtgaa aattcgcctg
    10681 ggcaactgcg actatctcga tccggcgggc aaggaatcac accgcaactt ccgcgagttc
    10741 tgggccctgc acctggcggc tgacctgatg ccggatgcag tcaccgccaa gaccgacgcc
    10801 gagggtcgat tcaatctggc ggccgcgccg ccggaaacgg tttgctggct gctgattgag
    10861 catcccgact ttgccaagat ttcgctgtat accgcaacca cggccgcgcc gcccaccgac
    10921 cacgacaagc accccgtgca caagctgccc ctggagattt ccttgcgggc ggcaaagtcg
    10981 atccctctct cggtcgtttg ggccgatacg ggcaagccgg ccgcgggggt gagagtccac
    11041 gcgtcccaaa tgcgggccag cggcagcgcg tcgtacggca caacggacgc cgccggcaag
    11101 ttcgtcctca agctaccctc cgggacctac aagctcacgg gcgatccgcc gcgcgactct
    11161 gactatgtcc gcaccacggt tgagttgaaa gtcaaagaag cgcccgcaga gcagccggcc
    11221 agcctcgcga tcgaccgcgg ggcggtgctc ctcttcaaag ctacggattt cgacaccggc
    11281 gctccaatcg cccgcgtttc cttctggtac gagccggagg atcgccccgg cagccgtttt
    11341 ggcgtgcagc ccaatcccag catcgtcacc catcccaaga cgaacgaaaa gggagaaatg
    11401 cgagtcgtcg ctgcgccggg cacgcgcaag tacggcgtcg gctttaaccc ccttcccgcc
    11461 ggctacgaac cgtcggtcgc caccgattcc acacccggcc gcgaactggt cctctccgcc
    11521 ggcgaagagc gcgtcgtgga gtttttgctc cgcaaacgcg agaagtgatg agcgcatgac
    11581 ttcgtgccgg ccgcgaggat gccgcttgcg cgcatcatgc gcaatttctg cctggtttgt
    11641 ttttcgtcgc aaccgattta cccacaagcg cttacggcga aagatgcccg cccaagattt
    11701 atccggttga tgcgcgcatc aactggaccg aagcgcgatg tagtgaatgg tgaaatcgac
    11761 caccgcgaga atgctcaccg aggcgatccc cgtgtagtgc agccagtcgc gcggcacgcc
    11821 gcgcaggcga tcggccaatt cggcgcccca gactggcggc agggccagcg cgacggaggc
    11881 caggtgggcg ccgatcggca ggtaccacgt ccaggttccg gcgctgaagc ggatgccgat
    11941 cagcgaggtg atgtcggccg cggcgttcag cccgagggcg aagacgatga tccaggctgc
    12001 cgtggtccag gtccgcgagg ccccgcgcag ccgagtcagg acgaccagtc cgatcagcgt
    12061 ggcaatactc caggccgcca attgttggca cagccaccaa acgaacgctt cgcggacgcc
    12121 gagcttcagt gccagaaggt acggcgcggt agttcccatg tcgatgagca ttcccaatcc
    12181 gccgaagatc agcagccagt ggccgggctg cgtcggcgct gggccgctgc cggttcgcca
    12241 ccgccagagg aataatccca ggccgctggc cgccgtgccg taggccaggc ccaggcccaa
    12301 gtgtcgcagc aagtcgggca gcgtcggcgt cgccggcata ttgaggcggt aaatggccag
    12361 caccgcgccg cagccgagca tccagcccag caagtggatg atgcccaacc gagttgtcgg
    12421 cgaactgagt tccctggtgt ccacgccgct atcctaggca gccgcgcagg catgcgtcaa
    12481 aataacgcga gcttgtcgtc tgactcccga ctccctttcc cgactcccga ctgccatgag
    12541 cctcatcgcc tccgtccacg cccgccagat tctcgacagc cgcggcaatc cgaccgtcga
    12601 ggtggaagtg accctctccg acggctcgtt cggaagggct gccgtgccca gcggggccag
    12661 caccggcatg cacgaggcct gggagctgcg cgacggcgac aaggccgtct acatgggcaa
    12721 gggcgtgacc aaagccgtcc acaacgccaa cacgatcatc gccgactcgc tcaccggctg
    12781 ggacgcgctc gatcagatcg gcgtcgatca gcggctgatt gagctcgacg ccacacccaa
    12841 caagaaaaac ctgggggcca acgcgatcct gggtgtctcg ctggcggtgg ccaaggcggc
    12901 ggccagtttc acgcagcagc cgctgtatcg ctatctgggc ggcgtagggg cgaacctgct
    12961 gccggcgccg atgatgaaca tcatcaacgg cggtgcgcac gccgacaatt cgatcgactt
    13021 gcaggagttc atggtcatgc cgctgggctt tcagcggttc ag
//
LOCUS       DASRJB010000586         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_168422, whole genome shotgun sequence.
ACCESSION   DASRJB010000586 DASRJB010000000
VERSION     DASRJB010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168422"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB80_15500"
     CDS             complement(<1..574)
                     /locus_tag="VFB80_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN35233.1"
                     /translation="MFASLSRRRFLALSAAAAAGATWFDVPRVLAAANLDDPKDAWGG
                     FPVGVQSYSLRNFSAEEAIRHLQGMGVHYVELFKKHLDPDGTPEQDAAVKKLVTAAGI
                     TISAHGVNDFTKDHEANKRLFDFAKRIGFKTITANPRPEAFESLDKLVAEYDIRIAIH
                     NHGPGALYDKLAGLQKAIAGHDKRIGACVDC"
     gene            752..>3150
                     /locus_tag="VFB80_15505"
     CDS             752..>3150
                     /locus_tag="VFB80_15505"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZN35234.1"
                     /translation="MLEFLLPLMLVAQPDPAPPEWQQDAELTAVTFVDADRGWAVGDR
                     GAIWHTDDGGRNWRLQRSSATCRLEAVQFLDGNNGWAAGGWTQPYTHETRGVVLRTRD
                     GGRTWQDTPGLTLPGLKQLKMIDSRQGWALGDPSTLFPSGVFRTDDGGRSWSPVPKGE
                     TAGWITGDFRDGRGGAVAGLGGMVATVTSGEVKPSRTPEADPRFLRRLLLCGEAGGWL
                     VGDGGLALTTADGGCHWTPPPGALPPVAGDVDLRALAVRGNRVWIAGAPGSCVLHSPD
                     NGRTWLSFRTDQTAPLLGLWFADDYRGWAVGALGTILHTKDGGQTWRLARSGPTRAAL
                     LGIFSEPQRIPLELIASQAGAEGYLTAVEILGRRDLEPGASTSAFTEIRRTHAAVVAA
                     GGSGADTSWRFPLAEPGLVQSGESILARWNEASGGHAAEQLEEHLVRRIRQWRPDVIV
                     TEDVSPRGDNPLAHLTNQLTLAAVAKAADDTAWPQQISRTGLAAWRVKKVLAVLPADK
                     PGVVNLTPAQWSQPLGCSIADQAARGRALLVSDVLSPPRTVGLSLLVNQLPQGSGRGD
                     VLSGISLAFGSDARRLAVKPPAGDLKALAEVARKRNLIDQLLSRIGEDHTVGAGWLAQ
                     VGDLTQGLSNQQAGEIIWQLAFKYHATGKSSQAAEALEYLLERHAQHPLADAAARWLV
                     QYYASSEVAWRERQESKFEVQVAAATAQAAEPAAGRANPAPAIARTGFSSTAGNGLNP
                     TQRAGRALALARQIEQSRPLLNADPAVRFSLATAWRQSGQPRSADRLFQLMTSSGGQS
                     S"
BASE COUNT          495 a         1081 c         1096 g          478 t
ORIGIN      
        1 cgcaatcgac gcaggcccca atccgcttgt cgtgacccgc gatcgccttc tgcaggccgg
       61 cgagcttgtc gtacaacgcg cccggcccgt ggttgtgaat tgcgatccgg atgtcgtact
      121 cggcgacgag cttgtcgagg ctctcgaacg cctcgggccg cgggttggcg gtaatcgtct
      181 tgaagccgat ccgcttggcg aagtcgaaca gccgcttgtt ggcctcgtgg tccttggtga
      241 agtcgttcac gccgtgagcg ctgatcgtga tgccggccgc cgtgacgagc ttcttcacgg
      301 ccgcgtcctg ctcgggcgtg ccgtccggat cgagatgctt cttgaacagc tcgacgtagt
      361 gcacgcccat cccctgcaaa tgccggatcg cctcttcggc gctgaagttc cgcagcgagt
      421 aactctgcac gccgacgggg aagccgcccc aggcgtcctt cgggtcgtcc aggttggccg
      481 ccgccagcac ccgcgggaca tcgaaccacg tggcccccgc cgccgcagcg gccgacaagg
      541 caagaaaccg gcgacgagag aggctggcga acatgggcaa agtcctggcg agtgaatgga
      601 agcgatacaa cggcccgatt atgggcagcc gctggccgcg aggcaatcaa gggttcgggg
      661 ttcagccaag ggttcggggt tcagggttca gggttcagga ttgtatgatc cgccgcctcc
      721 cgaaccctga acccccaacc ctgaaccgtc gatgcttgaa ttcctcctgc cactaatgtt
      781 ggtcgctcag cccgatcccg cgccgcccga gtggcagcag gatgccgagc tgacggcggt
      841 gacgtttgtc gatgccgatc gcggctgggc cgtcggcgac cgcggggcga tctggcacac
      901 cgacgacggc ggccggaatt ggaggctgca gcgctccagc gccacgtgcc gtctggaggc
      961 agtgcaattt ctggacggca acaacggttg ggcggcaggc ggctggacgc agccttacac
     1021 gcacgaaacc cgcggcgttg tgctgcggac gcgcgacggc ggccgcacgt ggcaggatac
     1081 gcccggcctc acgctgccgg ggctcaagca gctcaagatg atcgactcgc ggcagggctg
     1141 ggcgctgggc gatccgtcca cactcttccc ctccggagtg tttcgcaccg acgacggcgg
     1201 acgcagctgg tcgccggttc ccaaggggga aacggcgggc tggatcacag gcgactttcg
     1261 cgatggacgc ggcggcgcgg tggccggcct cggcgggatg gtcgcgacgg tgacgagcgg
     1321 cgaagtaaaa ccatcgcgca cgccggaggc agacccgcgc tttttgcggc ggctgctact
     1381 ctgtggcgaa gccggcggct ggctcgtcgg cgacggtgga ctggcgctga cgaccgccga
     1441 cggcggttgc cactggactc cgccgccggg tgccctgcca ccggttgcgg gcgacgtcga
     1501 cttgcgggct ctggccgtgc gcggcaatcg cgtctggatc gccggagcgc cgggcagctg
     1561 cgtgctgcac tcgcccgaca acggacgcac ctggctcagc tttcgcacgg accagaccgc
     1621 gccgcttctg ggcctgtggt tcgccgacga ctatcgcggc tgggccgtcg gcgcgctggg
     1681 cacgatcctg cacaccaagg acggcggcca aacctggcgg ctggcgcgca gcggccccac
     1741 gcgggccgcg ctgctgggga tcttcagcga accgcagcgc attccgctgg agctgatcgc
     1801 ctcgcaagcg ggcgccgagg gatatctcac tgccgtggaa attctcgggc ggcgcgacct
     1861 ggagcccggc gcaagcacgt cggcgttcac cgagatccgc cgcacgcatg cagccgtcgt
     1921 cgccgccggc ggcagcgggg ctgacacgtc gtggcgattt cccttggcag aacccggact
     1981 ggtccagtcg ggtgaatcga ttttggcccg ctggaatgaa gccagcggcg gacacgcagc
     2041 cgagcagctc gaggagcacc ttgtccgccg tatccgtcag tggcgacccg acgtgattgt
     2101 taccgaggac gtcagccccc gcggcgacaa cccgctggcc catctgacga accagctcac
     2161 actggccgcc gttgccaagg cggccgacga tacggcctgg ccgcagcaga tttcgcggac
     2221 gggcctggcg gcgtggcgcg tgaagaaagt cctggctgtg ctgcccgccg acaagcccgg
     2281 cgtcgtcaac ctcactcccg cccagtggtc gcagccgctg ggctgctcga ttgccgacca
     2341 ggccgcccgc ggccgggcgc tgctcgtatc cgacgtgctc agcccgccgc ggaccgtcgg
     2401 cctgtcgctg ctcgtcaatc agctgccgca gggaagcggc cgcggtgatg tgctgagcgg
     2461 catttcgctg gccttcggca gcgacgcccg ccgcctcgcg gtcaagccgc cggccgggga
     2521 cttgaaggcc ctggccgaag tggcccgcaa gcgaaatctg atcgaccagc tcctctcgcg
     2581 gattggcgag gaccacaccg tcggcgcggg ctggctcgct caggtcggcg atctcacaca
     2641 gggcctttcc aaccagcaag ccggtgagat catctggcag ctggccttca agtaccacgc
     2701 cacgggcaaa agcagccagg ccgccgaagc cctggaatac ctgctcgagc ggcacgcgca
     2761 gcatccgctg gcagacgccg ccgcccggtg gctcgtgcag tactacgcca gcagcgaagt
     2821 cgcctggcgc gagcgccagg agtcgaaatt cgaagtgcag gtggctgccg cgacggcaca
     2881 agccgcggag ccggcagcgg gccgggccaa ccccgcgccg gccatcgccc gcaccggctt
     2941 cagcagcacc gctggcaacg ggctgaatcc gacgcagcga gcgggacgtg ccctggccct
     3001 ggcccggcag atcgagcaga gccggccgct gctcaacgcc gatccggccg tgcggttttc
     3061 gctggccacg gcctggcggc agtccgggca gccgcgatct gcggatcgcc tcttccagtt
     3121 gatgacgagc agcggcgggc agagctcgtg
//
LOCUS       DASRJB010000587         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2424878, whole genome shotgun sequence.
ACCESSION   DASRJB010000587 DASRJB010000000
VERSION     DASRJB010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424878"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /gene="hisS"
                     /locus_tag="VFB80_15510"
     CDS             complement(<1..1268)
                     /gene="hisS"
                     /locus_tag="VFB80_15510"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZN35235.1"
                     /translation="MTLIEPRTLKGFRDYLPEAMIPRERLIATAQQVYRSFGYSPIDT
                     PALEYFEVLAGKGGDESDRQMYAFDDHGGRKVGMRFDLTVPLARFAAQHASELGLPFK
                     RYHIATVWRGENTQRGRYREFMQCDFDTIGTRSVAADIETALVIHDLIRRIGAAFGKP
                     LGFTIRVNNRAVLSGLLAKLGLSDKSTQILRALDKLGKIGREKVAAEMVAAAGTSQEQ
                     ADQVLRLAELSGTNDQILSQLGPLVAGNPQGEEGVERLRQILAGAKAGGVADGALLLD
                     VSIARGLDYYTGAIFETFLADLPTIGSICSGGRYDNLAGLFTKQELPGIGASLGLDRL
                     LAALEELGVIDKVRTPAPVLITYFDAARLDDYLRIAATIRAAGIGVEVFPEPKKLGQQ
                     LAYADRRGFRVALIAGQREFDAGTIQLKDL"
     gene            1835..3400
                     /locus_tag="VFB80_15515"
     CDS             1835..3400
                     /locus_tag="VFB80_15515"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN35236.1"
                     /translation="MRLRPIIQSLVLALAASWPAAAAPPTEAEMAAKIDARLAEVWAR
                     EKVEAAPPAEDAEFLRRAWLDLCGTIPPLNDPDGISGIRQFLDSNDPDKRVRLIERLL
                     ARPSHATHFANVWKNVLLPQDAEVQRLGGEAGFQSWLRGQFADNVPYDMLIASLVLAQ
                     GQANQAGPALFFTSLQLKPEELAASTSRVFLGTQIQCAQCHDHPFDHWKKADFWGYAA
                     FFARLERPQQAQQIAFQVSDAATGEVMIPGSSDAVTPRFLGGDTSGDDLGKSRRERLV
                     QWLTWPENPFVARAAVNRVWALLFGRGLVHPVDDLGRHNASAHPELMSELAEYFVRSG
                     YDLNRLVRTLTQTKAYQLSSRSPPGAEDRPELFARMSIKSLTAEQLYDCLLEAMRRRE
                     SASPAPQSALVLNRGLDATRQAFLMRFRAPTQGATEYEGGIPQALTLMNGTNIRQATD
                     LNQSDLLGALDAPFFTAAQKVEVLFLSTLSRPPRSEERAKFVAYVEQAGNRQALGDVL
                     WALLNSAEFMLNH"
     gene            3411..4724
                     /locus_tag="VFB80_15520"
     CDS             3411..4724
                     /locus_tag="VFB80_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35237.1"
                     /translation="MSEHSRRQFLRAAALSAAGVSCSGWMPRLARAAAGDPARRRHCV
                     LLWMPGGPSQMDTFDLKPGHANGGPFKEIETSVSGLKISEHLPKLAAQAENLAIVRSL
                     STAEGDHNRGTYLMHTGHKPGGPISFPTMGSTLSKALGTDDAELPNFISIAPQVALNS
                     AAYGPGFLGPRHAPLTVGSGNVIAPQQPAAGLGVDDLRNPGVGREQEDARLDLWRMLQ
                     TSFLAEHGSASPKAQNTVYERAVRMMRSEAAQAFDLAGESDKVKDAYGRGQFGQGCLM
                     ARRLIERGVPFIEIALRSNGGVLGWDTHQQNFDRVKSLSAELDAGWATLMTELHERGL
                     LESTTILWLGEFGRTPTINPGAGRDHFPKAWTTVLAGGGIKGGQAYGRTSADGGTVEE
                     NQVDVPDVLATLCRALGIDPATCNISELGRPIKIADGKPIEALLA"
     gene            4757..>5876
                     /locus_tag="VFB80_15525"
     CDS             4757..>5876
                     /locus_tag="VFB80_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35238.1"
                     /translation="MTRAAILGVVGALWLLGSASAQEATPQGTRARVPAAKALADKEK
                     AARADVEAARQEFKDAKGAQARLEAADKMAAAVARLVEAQKQFQRAQEAALQAAIRIP
                     SGRDVAERDVVHPDDPIERFALLTPGGPLVVEAALTIDGKPFRQAREQLIRDLIAAAD
                     KDKDGKTTWTEALASPRFTLGRINSPNEQTRTALIRNFDKNADEIVDPGEARKLIAQF
                     FQGGTFSLGSGAGFVRVLTANGVAFGGANNQANLLELLDTDHDKALDAKELAAAAGRL
                     KSRDADDNDLLAADEVSGVVASPAQRVDVRGVMPAVGAVLLGPAATQDGVYTLIYQRY
                     QKNRDNKLSADCFPLFPQLFAALDKDGDSEITKFEILGL"
BASE COUNT         1007 a         1976 c         1976 g          917 t
ORIGIN      
        1 tgcaagtcct tgagctggat cgtgccggca tcgaactcgc gctggccggc gatcagggcc
       61 acgcggaagc cgcggcgatc cgcgtaagcc agctgctggc caagcttctt gggctcgggg
      121 aagacctcga caccgatgcc tgccgcgcga atcgtcgccg cgatgcgcag gtaatcgtcg
      181 agccgggcgg catcgaagta ggtgatcagc accggggccg gcgtgcggac cttgtcgatc
      241 acaccgagct cttcgagcgc ggccagcagc cgatcgaggc cgagcgacgc gccgatgccg
      301 ggtagctctt gcttcgtgaa caatccggcg aggttgtcgt accggccgcc ggagcagatg
      361 ctgccgatcg tgggcagatc ggccaggaac gtctcgaaga tcgcgcccgt gtaataatcc
      421 aggccgcggg cgatcgagac gtcgagcaag agcgcgccgt ccgcgacgcc gccggctttc
      481 gcaccggcga ggatctgccg caggcgttcg acgccttcct cgccttgcgg attgccggcg
      541 acgagcgggc ccagctgcga gaggatttgg tcgttggtgc ccgaaagctc cgccagacgg
      601 agcacctgat cggcctgttc ctggctggtg ccggccgcgg cgaccatttc ggcggccacc
      661 ttctcgcggc cgatcttgcc gagcttgtcg agggcccgca ggatctgggt gctcttgtcc
      721 gagaggccga gcttcgccaa caggccgctc agcaccgcgc ggttattgac gcggatggtg
      781 aaccccagcg gcttgccgaa cgccgcgccg atccggcgga tgaggtcgtg gatgacgagg
      841 gccgtttcga tgtcggcggc gaccgagcgg gtgccgatcg tgtcgaagtc gcactgcatg
      901 aactcgcggt agcggccgcg ctgcgtgttc tcgccgcgcc aaactgtcgc gatgtgatag
      961 cgcttgaagg gcaggcccag ttcgctcgcg tgctgagccg cgaagcgggc cagcgggacg
     1021 gtcaggtcga accgcatgcc gaccttgcgg ccgccgtgat cgtcgaaggc gtacatctgc
     1081 cggtcggact cgtcgcctcc cttcccggca aggacctcga aatactccag ggccggcgtg
     1141 tcgatcggac tgtagccgaa agaacgatag acctgctggg ccgtggcgat cagccgctcg
     1201 cgcgggatca tcgcctcggg caggtaatcg cgaaagcctt tcagcgtgcg aggttcaatg
     1261 agcgtcatga cagcttccgc aggacaagcg ccttgcgaga ccggttcaag gcgaatcagg
     1321 cgcagggtca accgcggcta gcgtggggcg aaaatcggcc gcgactactt cttgcggtgg
     1381 cgaatgcgcg accgcatgcg gcgtttcttc cggcccagct tgcggcgacc gcgtcccgtc
     1441 cgtttgacac ccacgtagac aacccttgaa tggcttttgg tcgagcgaat tccgcatcgt
     1501 cggctgcggc gcatcaccgc cgccggcaac aacttgcggc aaggcgagca gtgtacccgc
     1561 ccgcggcgat tttgagcaag ggcgggcagg gcgaaccttt cgcctcccgc cgcgcactaa
     1621 ctggccgcgg aaaggagatt gaacagaagg aaacgaaggc aacgaagaga gggcgttgac
     1681 cgcgctgagt aatcgcccat ctgcctcctt cgttttcttc gtttccttct gttccaatgc
     1741 gttccaattc ccgccgaatc gttgcgactt gcgacgggtt atatagtaca acaacgactg
     1801 cgtattcctt ggggtgcctg gaaggaggaa tcgcatgcgt ttgcgaccaa tcattcagtc
     1861 gctcgtgctt gccctggcgg cgagttggcc cgccgctgcc gccccgccga ccgaagcgga
     1921 gatggcggcc aagatcgatg cccgcctggc ggaggtctgg gcccgcgaga aggtcgaggc
     1981 cgcgccgccg gccgaagacg ccgagttcct gcggcgggcc tggctcgacc tgtgcggcac
     2041 gatcccgccg ctcaacgatc ccgacggcat cagcggcatc cgccagttcc tcgattcgaa
     2101 cgatccggac aagcgcgtcc gcttgatcga gcggctgctg gcccggccgt cgcacgccac
     2161 gcactttgcc aacgtctgga agaacgtgct cctgccgcaa gacgccgagg tgcagcggct
     2221 gggcggcgag gccgggtttc aatcctggct ccgcgggcaa ttcgccgaca acgtgcccta
     2281 cgacatgctg atcgccagcc tcgttctggc tcaggggcag gccaaccagg ccgggccggc
     2341 gctgttcttc acgtcgctgc agctcaagcc ggaggagctg gcggccagta cgtcgcgggt
     2401 cttcctcggc acgcagattc agtgcgccca gtgccacgac catcccttcg atcattggaa
     2461 gaaggccgac ttctggggct atgcggcgtt cttcgcccgg ctggagcggc cgcagcaggc
     2521 gcagcagatc gcctttcaag tcagcgacgc cgcgaccggc gaggtgatga ttcccggctc
     2581 gagcgacgcg gtgacgccgc ggtttttggg cggcgacacg agcggcgacg acctgggcaa
     2641 gagccgccgc gagcggctgg tgcagtggct gacctggccg gagaatccgt tcgtggcgcg
     2701 ggccgccgtg aaccgcgtct gggcgctgct cttcggccgc ggcctcgtgc atccggtcga
     2761 tgacctgggc cggcacaacg ccagcgcgca tccggagctg atgtccgagc tggccgagta
     2821 cttcgtccgc tcgggctacg acctgaaccg cctcgtgcgg acgctgacgc agacgaaggc
     2881 ctatcagctt tccagccgct cgccgccggg cgcggaggat cgacccgagc tgttcgcccg
     2941 gatgtcgatc aagagcttga cggccgagca gctctacgat tgcctgctcg aagcgatgcg
     3001 gcggcgcgaa tccgcgtcgc cggcgccgca gtcggccctg gttctgaacc gcggcctcga
     3061 cgccacgcgg caggcgttcc tgatgcggtt ccgcgcaccg acgcaggggg cgaccgaata
     3121 cgaagggggc attccgcagg ctctcacgtt gatgaacggc acgaatatcc ggcaggccac
     3181 cgacctcaat caaagcgatt tgctgggggc cctggacgcg ccgttcttca cggccgcgca
     3241 gaaggtggaa gtgctgttcc tctcgacgct gtcgcggcca ccgcggagcg aagagcgggc
     3301 gaagttcgtg gcctacgtcg agcaggccgg caatcgccag gcactgggcg acgtgctctg
     3361 ggcgctgctc aacagcgccg aattcatgct gaatcattga ggtgacaatc atgtccgaac
     3421 attcgcgccg gcaatttctc cgtgcggctg cgctgagcgc ggccggtgtc agctgcagcg
     3481 gctggatgcc gcgcctggcg cgggcggcgg cgggcgatcc ggctcgccgc cggcactgcg
     3541 tgctgctctg gatgcccggc ggccccagcc agatggacac cttcgatctc aagccgggcc
     3601 acgccaacgg cggaccgttc aaggaaatcg aaaccagcgt ctcggggctg aaaatcagcg
     3661 agcacctgcc gaagctcgcg gcccaggccg aaaacctggc gatcgtccgc agcctcagca
     3721 ccgccgaggg agaccacaac cgcggcacgt acctgatgca cacgggccac aagcccggcg
     3781 gaccgatcag ctttcccacg atgggctcga cgctctccaa ggccctggga accgacgacg
     3841 ccgagctgcc caatttcatc agcatcgcgc cgcaggtcgc actcaacagc gccgcgtacg
     3901 gccccggctt cctcggcccg cggcacgcgc cgctgaccgt cggctccggc aatgtgatcg
     3961 ctccgcagca gccggcggcc ggcttgggcg tcgatgacct gcgaaatccg ggcgtcggcc
     4021 gcgagcagga agacgcccgc ttggacctgt ggcgaatgct ccagaccagc ttcctcgcgg
     4081 agcatggctc cgcctcgccg aaggcccaga acacggtgta cgagcgggcg gtgcggatga
     4141 tgcggagcga ggccgcccag gcgttcgacc tggccggcga gagcgacaag gtcaaggatg
     4201 cgtacggccg cgggcagttt ggccagggct gcctgatggc gcgccgcttg atcgagcgcg
     4261 gcgtgccgtt catcgagatc gccctgcgga gcaacggcgg cgtcctcggt tgggacacgc
     4321 accagcagaa cttcgaccgc gttaagtcac tgtcggccga gctcgacgcc ggttgggcga
     4381 ccctgatgac cgagctccac gagcgcggcc tgctcgaatc gacgacgatc ctctggctgg
     4441 gcgagttcgg ccgcacgccg acgatcaacc ccggcgccgg ccgcgaccac ttcccgaagg
     4501 cctggacgac cgtgctcgcc ggcggcggca tcaagggcgg ccaggcgtat ggcaggacta
     4561 gcgccgacgg cggcacggtc gaagagaacc aggtcgatgt gccggacgtg ctggccacgc
     4621 tctgccgggc gctgggcatc gatccggcca cgtgcaacat ttccgagctt ggccgcccga
     4681 tcaaaatcgc cgacggcaag ccaatcgagg cgctgctggc gtagcggcgg acggaattca
     4741 tcgcggagat ccactcatga ctcgtgctgc aattctcggc gtcgttggcg ccctctggct
     4801 gttgggatca gctagcgccc aggaagccac cccgcaaggc acgcgcgctc gagtccccgc
     4861 ggccaaggcc ctggccgata aggaaaaagc cgcgcgggcc gacgtcgaag ccgcgcgtca
     4921 ggagttcaag gacgccaagg gtgcccaggc ccgcctggag gccgcggaca agatggccgc
     4981 cgcggtggcc cggctcgtcg aagcccagaa acaattccaa cgggcgcaag aagccgcgct
     5041 ccaagccgcc atccgcatcc cttccgggcg cgatgtggcc gagcgcgacg tcgtccatcc
     5101 cgacgatccg atcgagcgat tcgcgctgct cacgcccggc ggcccgctgg ttgtcgaggc
     5161 ggcgctgacg atcgacggca aaccgttccg ccaggcccgc gagcaactga tccgcgactt
     5221 gatcgccgcc gccgacaagg acaaagacgg caagaccacc tggaccgagg ccctcgcctc
     5281 gccgcggttc acgctgggcc ggatcaactc gcccaacgag cagacgcgca ccgcactgat
     5341 ccgcaatttc gataagaacg ctgacgagat tgtcgatccc ggcgaagccc gcaagctgat
     5401 cgcgcagttc tttcaaggcg gtacgttctc gctcggcagc ggcgcgggct tcgtccgcgt
     5461 gctcacggcc aacggcgtgg ccttcggcgg cgcaaataac caggcgaacc tgctggagct
     5521 gctcgacacc gatcacgaca aggcgctcga cgccaaggaa ctggccgcag ctgccggccg
     5581 cctcaagagc cgcgacgccg acgacaacga cctcttggcg gctgacgaag tttcgggcgt
     5641 cgtcgcctcg cccgcgcagc gcgtggacgt ccgcggcgta atgcccgcgg tcggagccgt
     5701 gctgctgggt ccggccgcga cgcaggacgg cgtctacacg ctgatctacc agcgctacca
     5761 aaagaaccgt gacaacaagc tgagcgccga ctgctttccc ctcttcccgc agttgttcgc
     5821 ggctctcgac aaggatggcg acagcgagat caccaagttc gagattttag ggctga
//
LOCUS       DASRJB010000588         6904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_169511, whole genome shotgun sequence.
ACCESSION   DASRJB010000588 DASRJB010000000
VERSION     DASRJB010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169511"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1697)
                     /locus_tag="VFB80_15530"
     CDS             complement(276..1697)
                     /locus_tag="VFB80_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN35239.1"
                     /translation="MRIFYAGQWRDGDAKIEVRNPFDGSIVDTVPQVGAAEVDAALAA
                     AVEGAKVMRRLPGYERFKILRKAADILLARQNDLGRIISLEEGKTLAEGIFEVTRAAE
                     TIELSAEEAKRLGGEVLPLDGAPGGAGKLGFTIRVPCGVVVAITPFNFPLNLVCHKVG
                     PALAGGNAVVIKPASNTPLSALKLVEILLEAGLPPLAIACLTGGGGELGKALCSDARV
                     RKISFTGSVEVGQQIVSVAGIKRVTMELGSNSPLIVLDDADLNKVADAVVATGYGNAG
                     QVCISTQRVIALDSIYPDLLEALKPRVEGIQAGNQLAEGTKMGPMIRERDAVRVGQWI
                     EEAVQQGARLVAGGSRQGTLHQPTLLADVQPHMKVAQDELFGPAVGVSRARDVEQAIA
                     LANHSRYGLSAGIFTQDIDAAIRFAREVDAGNLHINWGPAWRADLIPYGGLRDSGFGK
                     EGPKYAIHEMTEMKTVIVHTRAS"
     gene            1944..2243
                     /locus_tag="VFB80_15535"
     CDS             1944..2243
                     /locus_tag="VFB80_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35240.1"
                     /translation="MAKFYVQSGSLELVLQAHDSRCAAIWAVHRTLSPALPFLCEEAG
                     DYLETADARRLGETIRVSERGFGSPSGHWFDTLDIVTEWNQLLVALDRLQERVNS"
     gene            2356..2823
                     /locus_tag="VFB80_15540"
     CDS             2356..2823
                     /locus_tag="VFB80_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN35241.1"
                     /translation="MAAKPVPDGYHTVTPYLVMQGAAKAIEFYKRAFGASELLRLAGP
                     GGTIGHAEIKIGDSIIMLADEHPQMGYRGPQALGGTPVSILLYVANVDALFAQAIAAG
                     GKATRPVADQFYGDRSGTLTDPFGHVWTISTHKEDVSQEEIERRFAELSKGGK"
     gene            2918..3583
                     /locus_tag="VFB80_15545"
     CDS             2918..3583
                     /locus_tag="VFB80_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HZN35242.1"
                     /translation="MIQPGCAICSRRMFLGGLTLSAASLWTPGVFAQELTRTPDQTEG
                     PFYPDKLPLDTDNDLLIINDSLTPAVGEITYVSGRILDRLGKPIKNAAVEIWQCDQNG
                     AYLHSGTSNADKKDRNFQGFGRFTTAASGEYLFRTIKPVPYPGRTPHIHFKIKLKGKD
                     LLNTQCYIKDHPGNARDGIYQSVRDEKLRQAITVDFTPLKDSKLGELSAKFDIVLGVT
                     PEA"
     gene            3659..4507
                     /locus_tag="VFB80_15550"
     CDS             3659..4507
                     /locus_tag="VFB80_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZN35243.1"
                     /translation="MRWQGNRQSENVEDRRGARRPLMMGGGIGGIILLLLVLFLGGPQ
                     AVMQLLQQQGGGPVAGPQVDEDPQATAFVRTVLADTEDVWDEQFQAVGRAYQKPKLVL
                     FSDEVDSACGFASAAVGPFYCPADRKVYLDTSFFRELEQRFEAPGEFAQAYVIAHEIG
                     HHVQNQLGWSDRVQNAQQRVGKVEANKLSVRLELQADYLAGVWAHHAQRSRNILERGD
                     IQAALDAATAIGDDKLQMESRGRVVPDSFTHGTSAQRARWFKRGLQTGDLAGAELLFQ
                     VDDSEL"
     gene            complement(4574..>6904)
                     /locus_tag="VFB80_15555"
     CDS             complement(4574..>6904)
                     /locus_tag="VFB80_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35244.1"
                     /translation="APVQLAATGMQGAALSGDGSLLAVPSPAPAPALGTAVVNVYSTR
                     EPNLPDEFTTELWSADWVALSADGSRLAVLGHDAAECYDVKTKRRTGRIPVDVRPIAP
                     SMLPTFMRGYRRGSEAALSPDGSRLATYKGGQITLYDLPSSKIAYTLTGGPRAIQIVI
                     FTLGFAAWAAGWGIVAKRERLRQPPAAPREEARPAATRPVPTVQSPWRSIGSALIWVI
                     MIAAFLAVFSTVGRALSLRFVLDMLLTLLGGLAAVVAIIFVYFWLARLFMGPHYFTLL
                     RLRQVSGGVGRTFPQGDVQFWFAAPSPYEGHQVGQVFDEARQRARELFGDDGPRSVPA
                     TLVAWLDRQCELDAFVGRATPVPAIVPNIWRGNSAVVCEETALRRFGHPLTALRAALA
                     LMFAIRRKRGLLPGYVGGLVSRELCWDEESPADVRAAARRLKLLLARCPDFSPQRVLL
                     RPPNERRDLWFTLDLPESWREVRAEVDLLATLHPLLFGTTAPAGRRERVLAWLRAIKP
                     KDDPLATMKQAAGLSLDELLAEWRAWLDSADTTYDSPSSERQAQLQTLIVRQILDRSQ
                     PALDRAKAARNLGGTSFVGAAGSLISLLSDPRCEFRGEVVDALENLSGLPLGDDLVAW
                     QAWWTALPAELRWQTAEPPIVAELVEKAGAACALGSPSEVLAAKPNLPAPVELKVVWG
                     LMAIGGLTALAIPITFLFVVGPLVFVTIYLSLIVGVRAMIFGAARETQGLAGVAKLQM
                     LNIIACDPINLLLATLEQTLLRRPNVQEYLAQAGGR"
BASE COUNT         1240 a         2302 c         2251 g         1111 t
ORIGIN      
        1 cgttttcgct ggcaggcctg ccgaagcaat gaggcggtct gtccggagag gtgtacggcg
       61 gaggcgccag cgatttccgt cgccagaaaa tcagagcggc gtttctcaca gaagccctcg
      121 aatgaagcga ggcgagggca aggcgaaacg agcgaccagc gaagccgtca ttctaggacg
      181 gggcccgaag ttgggaagcc gtcggcgacc gggggcgatt cagaattcgc gcagcaagtc
      241 ccccgaccat ctcagcatct acatacgttg aacgatcaac tcgcccgcgt atgaacgatg
      301 accgtcttca tctcggtcat ctcgtggatg gcgtacttgg ggccctcttt gccgaacccg
      361 ctgtcgcgga ggccgccgta ggggatcagg tcggcccgcc aggctgggcc ccagttgata
      421 tgcaggttgc cggcatcgac ttcgcgggcg aagcggatcg cggcgtcgat gtcttgcgtg
      481 aaaatgcccg cgctgaggcc gtaccgcgag tggttggcca aggcgattgc ctgctccacg
      541 tcgcgggccc ggctgacgcc gaccgctggc ccgaacagct cgtcctgggc gaccttcatg
      601 tgcggctgca cgtcggcgag cagcgtcggc tggtgcagcg tgccctgccg cgagccgccg
      661 gcgaccagcc gcgcaccttg ctgcacggcc tcctcgatcc actgcccgac gcggaccgcg
      721 tcgcgctcgc ggatcatcgg gcccatcttc gtgccctcgg ccagttggtt gccggcctgg
      781 atgccttcga cgcgcggctt gagcgcttcg agcaaatcgg gatagatgct atcgagggcg
      841 atcacccgct gggtggaaat gcagacttgg cccgcgttgc catagccggt ggctacgacg
      901 gcgtcggcga ccttattcag gtcggcgtcg tccagaacga tcaaggggct attgctgccc
      961 agctccatcg tgacgcgctt gatgccggcg accgagacga tttgctggcc gacctcgacg
     1021 ctgcccgtga agctgatctt gcggacgcga gcatccgagc aaagggcttt accgagttcg
     1081 cctccgccgc cggtaaggca agcgatcgcc agcggcggca ggcccgcttc cagcagaatc
     1141 tcgaccagct tgagcgccga cagcggggtg ttgctggccg gcttgatgac gaccgcgttg
     1201 ccgcctgcca gcgcggggcc gaccttgtgg caaacgaggt tcagcggaaa gttgaacggc
     1261 gtgatcgcga cgacgacgcc gcaggggacg cggatcgtga agccgagctt gcccgcgccg
     1321 cccggagcgc catccagcgg cagcacttcg ccccccagcc gcttggcctc ttctgccgac
     1381 agctcgatcg tctccgcggc ccgcgtgact tcgaagatgc cttcggccag cgttttgccc
     1441 tcctcgaggc tgatgattcg ccccagatcg ttctgccggg ccagcagtat gtccgcggcc
     1501 ttccgcagga tcttgaaccg ctcgtagccg ggcagccggc gcatgacctt cgctccttcg
     1561 accgccgcgg ccagcgccgc gtccacttcg gccgcgccga cctgcggcac cgtatcgacg
     1621 atcgagccgt cgaagggatt gcggacctcg atcttggcgt ctccgtcgcg ccactggccg
     1681 gcatagaaga tgcgcatcgt aggcagacct ttctggtgac taccgtggag tccggagcga
     1741 tttacgatcc agatttttct ttccgcaagt cattggcagg gcggcggtta accacgcatc
     1801 aacaaaaccg aatggaccac actagtccaa agctcggcga ggaagtcggt agatcggtcg
     1861 gtcggtggtc ggtaagtcgg tgaagccgca ccgaccaacc gaccaccgac tgactgaccc
     1921 acctcaaatc cctggagaca cccgtggcca agttctacgt tcaatctgga agcctcgaac
     1981 tcgttctgca agcccacgac agccggtgtg cggcgatttg ggccgtgcac cgcacgttgt
     2041 cgcccgcgct gccatttctg tgcgaagagg caggcgacta tttggagacg gccgatgccc
     2101 gccggctggg cgaaacgatt cgcgtcagcg agcgcggctt tggcagcccg agcggccact
     2161 ggttcgacac gctggacatt gtcaccgagt ggaaccagct gctggtggcc ctcgaccgct
     2221 tgcaggaacg tgtcaatagt tgacctcacg ctccgcgtga ggcaaacatc tcgcggagcg
     2281 agatgactac ttaccaatgg cagcgcgctc ggcgctatgc gacaattcgt attcctcgtc
     2341 tcccaggaaa ttcccatggc ggccaaaccg gttccggatg gatatcacac cgtcacgccc
     2401 tacctcgtca tgcaaggcgc ggcgaaggcg atcgagttct acaagcgggc cttcggggcg
     2461 agcgagctgt tgcggctggc aggcccgggc ggcacgattg gccacgccga gatcaagatc
     2521 ggcgattcga tcatcatgct ggccgacgag catccgcaga tgggctatcg cggaccgcag
     2581 gccctgggtg gcacgccggt cagcattctg ctgtacgtcg ccaacgtcga tgccctgttc
     2641 gcgcaggcga tcgccgccgg cggcaaggcg acgcgtccgg tggccgatca gttctacggc
     2701 gaccgctccg gcacgctgac cgatccgttc ggccacgtct ggacgatctc gacgcacaag
     2761 gaggacgtgt cgcaggaaga aatcgagcgg cggtttgcgg aattatcgaa gggcggaaag
     2821 taacgtcaaa cgtccgtaac ggggatttgc cgcggcacgt tcgatccccg atgataggag
     2881 cgtccagcca actcttttct ttgaatcgga gcttgtcatg attcagccag gttgcgcgat
     2941 ctgctcgcgg cggatgtttt tgggcggcct cacgctctcg gcggcgtcgc tgtggacgcc
     3001 gggcgtcttt gcacaggagt tgacccgcac gcccgaccag accgagggcc cgttctatcc
     3061 cgacaagctg ccgctggaca ccgacaacga tctcctcatc atcaacgaca gcctgacgcc
     3121 cgcggtcggc gagatcacct acgtcagcgg ccggatcctc gaccgcctgg gcaagccgat
     3181 caagaacgcg gccgtcgaaa tctggcagtg cgaccagaac ggggcctacc tgcactcggg
     3241 caccagcaac gccgacaaga aggacaggaa cttccagggc ttcggtcgct tcacgaccgc
     3301 cgccagcggc gaatacctgt tccgcacgat caagcccgtc ccctatccgg gccgcacgcc
     3361 gcatatccac ttcaagatca aactcaaggg caaggacctg ctcaacacgc agtgctacat
     3421 caaggaccat cccggcaacg cccgcgacgg catttaccag agcgtccgcg acgaaaagct
     3481 ccggcaagca atcaccgtcg atttcacccc ccttaaggac tcgaagctcg gcgagctgtc
     3541 ggccaagttc gacatcgtgc tcggcgtgac gcctgaggcg taggcccgcc gctccccggg
     3601 acaatcggtg cggtatttgc agcacggatc gtcctattcc cggagcagga ggagttctat
     3661 gcgctggcaa ggcaatcggc agagcgaaaa cgtggaggat cgccgcgggg cgcggcggcc
     3721 gctcatgatg ggcggtggca tcggcgggat catcctgctc ctgctggtcc tgttcctggg
     3781 cgggccccag gcggtgatgc agctgctcca gcagcagggc ggcggtcccg tcgccggtcc
     3841 gcaggtcgat gaagatccgc aggcgaccgc gttcgtgcgg accgtgctgg ccgacaccga
     3901 ggacgtctgg gatgagcagt tccaggccgt cggtcgggcc taccagaagc cgaagctcgt
     3961 gctcttcagc gacgaggtcg attcggcctg cggctttgcc agtgcggcgg tcgggccgtt
     4021 ctattgcccg gccgaccgca aggtctatct cgacacttcg ttcttccgcg aattggagca
     4081 gcggttcgag gcccccggcg aattcgccca ggcctacgtc atcgcccacg agatcggcca
     4141 ccacgtgcaa aaccagctgg gctggagcga ccgcgtgcaa aacgcccaac agcgcgtggg
     4201 aaaggtcgaa gccaacaagc tctccgtgcg gctggagttg caggccgatt acctggccgg
     4261 cgtgtgggct catcacgccc agcggtcccg caacatcctg gagcggggcg acatccaggc
     4321 cgccttggac gccgccacgg ccatcggcga cgacaagctg caaatggaat ctcgcggacg
     4381 ggtcgtcccc gactccttca cccacggcac cagcgcccag cgagcccgct ggttcaagcg
     4441 cggcctgcag accggcgacc tcgccggcgc ggaactgctc ttccaggtgg acgactcgga
     4501 gctctgattg caaagccgcg accagtggtc gcggtcttac gaatacaccg cgaccactgg
     4561 tcgcggcttt gagctaccgc ccgccggctt gggccaaata ctcctgcacg ttgggccggc
     4621 ggagtagagt ttgctccaga gtggccagta gcaggttgat gggatcgcag gcgatgatgt
     4681 tgagcatctg gagcttggcg acgccggcca ggccctgggt ttcgcgggcc gcgccgaaaa
     4741 tcatcgcccg gacgccgacg atcaggctca ggtaaatcgt cacgaagacc agcgggccca
     4801 ccacgaacag gaacgtgatg ggaatcgcca gcgccgtcag gcccccgatc gccatcaggc
     4861 cccagacgac tttcagctcg accggtgcgg gaagattcgg tttcgcggcc agcacttcac
     4921 tcggcgatcc aagcgcacag gccgctcccg ctttctcgac caactcggcg acgatcggcg
     4981 gctcggcagt ctgccaccgc aattccgccg gcagcgccgt ccaccaggct tgccacgcaa
     5041 ccaaatcgtc acccaggggc aggccggaga gattctccag cgcgtccaca acctcgccgc
     5101 ggaactcgca gcggggatcg gacagcagcg aaatgagcga gccggccgct cccacgaacg
     5161 acgtgccgcc caggttccgc gccgccttcg cgcggtccag agccggctgc gagcgatcga
     5221 gaatctgccg cacgatcaag gtctgtagct gcgcttgccg ctccgaggaa ggcgagtcgt
     5281 acgtcgtgtc cgcggagtcg agccacgccc gccactcggc gagcagctcg tccagcgaca
     5341 ggcctgctgc ctgcttcatc gtcgccagcg ggtcgtcctt tggcttgatt gcgcgcagcc
     5401 aggcgagcac tcgctcgcgg cggccggcgg gcgcggtggt cccaaacagc agcgggtgca
     5461 gagtcgcgag caggtcgact tccgcgcgga cttcccgcca cgattccggc agatccaggg
     5521 taaaccacag gtcccgccgc tcgttgggcg gccgcagcag cacccgctgc gggctgaaat
     5581 ccggacagcg ggccagcagc aatttgaggc ggcgggcggc agcgcggacg tcggccggcg
     5641 attcttcgtc ccagcacaat tcgcgcgaga cgaggccgcc gacatagccg ggcagcaggc
     5701 cgcgcttccg gcggatcgcg aacatcagtg ccagggcggc ccgcagcgcg gtcagcgggt
     5761 ggccgaaccg ccgcagggcc gtttcttcac agacgaccgc agagttcccc cgccagatat
     5821 tgggtacgat cgctggaacg ggcgtcgcgc ggccgacgaa cgcgtccagc tcgcactgcc
     5881 ggtcgagcca ggccaccaaa gtagcaggca cactccgtgg gccgtcgtcg ccaaacagct
     5941 cgcgggcccg ttgccgcgct tcgtcgaaga cttgcccgac ctggtggccc tcatagggcg
     6001 aaggcgcggc gaaccagaac tgaacgtcgc cctgcggaaa agtccggccg acgcccccgc
     6061 tgacctgccg caggcgaagc agcgtgaagt agtgcggccc catgaagagc cgggccagcc
     6121 agaagtaaac gaaaatgatt gccacgaccg cagccagtcc gcctagcaaa gtgagcagca
     6181 tgtcgagcac gaagcgcagc gacagcgccc gtccgacggt tgagaacact gccaggaatg
     6241 cggcaatcat gatgacccag atcagcgccg agccgatcga ccgccacggc gattgcacgg
     6301 tgggcaccgg ccgcgtcgcc gccggccgcg cttcttcacg cggggccgca ggcggctgcc
     6361 gcaagcgctc gcgcttggcg acgattcccc agccggcagc ccaggccgcg aagccgagcg
     6421 tgaagatgac gatctgaatc gcccgcggcc cgccggtcag cgtgtaggcg attttgctgc
     6481 tgggcaggtc gtagagcgtg atctggccgc ctttgtacgt ggccagccgg ctgccgtcgg
     6541 gcgacaacgc ggcctcgctg ccgcgacgat agcccctcat aaacgttggc agcatgctcg
     6601 gcgcgattgg gcgcacgtca actgggatgc ggccagtgcg gcgtttggtt ttcacgtcgt
     6661 aacattcggc ggcatcgtgg ccgagcacgg ccaggcgcga gccgtcggcc gacagcgcga
     6721 cccagtcggc agaccacaat tcggtggtga attcgtccgg caagttgggc tcgcgcgtcg
     6781 aatacacgtt gacgacggcg gtgcccaagg cgggtgcggg agcagggctg ggcacagcca
     6841 gcaggctgcc gtcgcccgac agcgccgcgc cctgcatgcc cgtggcggcc agctgcacgg
     6901 gtgc
//
LOCUS       DASRJB010000589        10994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1582046, whole genome shotgun sequence.
ACCESSION   DASRJB010000589 DASRJB010000000
VERSION     DASRJB010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582046"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFB80_15560"
     CDS             <1..1146
                     /locus_tag="VFB80_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN35245.1"
                     /translation="PGFNGLTQEVPASGIGRNDYPGNFAEFLKQRPKEQPFCFWMGFT
                     EPHRAYEFDSGVRLGKKLADVKVPAFLPDTDIVRRDLADYAIEVEWGDQQIGRTLRIL
                     EDAGELNRTLVVVTSDHGMPFPYVKGQIHEDAFHLPLAMRWAGGIKPGRVVEDFVNVR
                     DLAPTYLELAGLAVHPQMTGKSLAAILRSDKSGWIENRDVMLVGKERHDIGRPNDLGY
                     PVRAIRTKDYLYVHNFHPERWPAGNPETDYPNCDAGPSKEVIKVLGGHFLEISFGKRL
                     PDELYRLSDDPECVRNLANDLAFKEVADELRYKMWTMLKNEGDPRAAGQTEIFDTYKY
                     TAARGKGYETWLQQQEAGPLAELKAKLEEAAKRPPAKGKAKKAKSSN"
     gene            1183..4077
                     /locus_tag="VFB80_15565"
     CDS             1183..4077
                     /locus_tag="VFB80_15565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN35246.1"
                     /translation="MKRSIFLPLVAAAMVSAHLSAADVLPHWIWTNEPGSQRATFQHN
                     FTAPSAVQSASLRAVADFCLAGIWINGRPVGAIEPYGPALDIDVTAAIRAGQNEIAIN
                     AGAVPGPAAIALRLTIVAADGTRRSIVTDETWRGAKSAGEALPALFGIGRRPPTIDPF
                     DNYEQWRQAIGASAASAAAFWTAPSFEITLVRAAPSEEGSWVSLAFDPQGRATIAREE
                     KGLLRMTLADDRRSVAKVETIDDTLLECRGLLYAHGALYASANNSKGLYRLRDADGDG
                     RFDETKLLRELSGSVGHGRNDLALGPDGSIYWISGDSVDLPRENVVDRTSPFREARRG
                     QTTREGFVLRTDRDGAKWELIAAGLRNPFGIALNEHGDAFTYDADAEFDMGSPWYRPT
                     RVVQLAAGSDFGWRGVTGKWPPYFPDHADNALPALDIGRGSPTAVMFGTDLKFPADYK
                     RSLLILDWTYGRILAVHLLPRGAGYRGAAETLLKGRPLNVTDLAAGPDGALYLVTGGR
                     KTQSALYRLAFTGAERQDSPPSPHEKACQDHAQASRAVRKQLQQLHQPSAAALDAAWA
                     YLNDHDPRLRYAAMVATEHQLVDQWRERALKEKRPMAALTALVALARGGDAAALEALL
                     ELPAGQFNVGQLLLLVQSYLLLNDAAPAEVASRKQRIIAQLDPLFPHPAAAASHVSPA
                     GTAASLNRDLARLLAALDAPGLVERVAKSLLASPAQENRLHGLFVLRNVKSGWTSDTR
                     RAYFTALNDGAKYVAGEGMPKFLAQIREQAVLALTDAERRELADLLEAKAIDDEPLPP
                     ARPLVKRWEAGDFEKLLADPDRRGDAERGSVVFREALCARCHRAGARGPAVGPDLTHV
                     AGRFSPRDLLDSILTPNRVVAENYRGVQVRTTDGRTITGRVLLEGDYRSEKLRLSTQP
                     LAPAEFVELDKKQIEEVRESPISPMPEGLLDGFRESDVLDLLTFLTRATR"
     gene            4116..6935
                     /locus_tag="VFB80_15570"
     CDS             4116..6935
                     /locus_tag="VFB80_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35247.1"
                     /translation="MAANRAGRLTIGLYLLALAAARVAPAQTPAEPVDFLAPAEVIVG
                     PTRLAWGGVLSPDESWFAVGYGHWSNELGRVCVFDRASGKLRWSAEEPRGVRGLAVSP
                     DGALVASGNFAGEIRLRDAASGAVRQTLLETGGSIEKLSFAHGSRRLATGSNGNAVRV
                     WDLATGQIERTLIGHTGRAYWIEFSPDDKLLVTASQDRSVRIWDAANGLLKHTLAHPG
                     EVSAAVFLPDGLQVATVCHDGLIRLFDIQSGALTMTLQDAGQPLAAKYALAVSADGTT
                     LAAGNSGQIQIWDLVGGKLRATLAGHRGLVFGISLSKDGRTLVSTGWDALAKVWDVAG
                     ERELRSIPLPASHHDSPGPIVGLALSPDGALAAAVLTDQTVQLRDRKSGKVVRTLTGH
                     SDVVLAAAFADNGKWLATSAANGEVRIWNVASGQLHKTLSGHKSAVYAASWSAGGKLL
                     ATAGDDKTVRLWNGDSFAPLATLSGHADAVRSLAFAPDAARLYSGSSDGTAIIWDVAK
                     RAAEGTLAGHPAAIRAIATSSDGVSVATGGEEGGVTVWNVATRQPRLKLAGHPMGGVL
                     SLAFSPRGQTLASSGADKNIRLWDPANGQMRKLFGAHAAEVTGVAFLPQAAGLVSAST
                     DGSLRLWKADEPRTQPVVSLPAHAPEAYCAKFSPDGQWLVTGGKNSLIALRDPVTGEV
                     KTTLTGHSGIIYDVAFSPDYLLLASAGSDGTVRLWSLNRAVEIMKYNGYGAKLAGIRS
                     VAFSPDGRTLASGAGDGTLKLFDVDSGVVRKSLPSQTLSVNSIRFTPDSTLMITATGD
                     WQQPNVAGELRVWEVASGQEFAKLPGHTVEIKRIDLSRDGKRLVSSGSDRTLMVWDLT
                     QRKVIGTVKTETVGTAIALLPDGQRLAMGDGRGGVWLWDLTAGKLLTRYAGHNKVIPG
                     IGVSPDGKLIASASQDGTLKLWPVR"
     gene            complement(6940..8064)
                     /locus_tag="VFB80_15575"
     CDS             complement(6940..8064)
                     /locus_tag="VFB80_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35248.1"
                     /translation="MGRTCNRCPPSHSRRMFLAASGLSLLGTSALAQERGADDCPQCG
                     GVGLVPIPDAKPFVWVDGQPAPKPEAAVGEQHCPVCRAGGDSAELVAAAKGRFERAQS
                     KHAEWKERIGGRLVLVMTRHAALTTQFTFAQAKSAGQAVESLTLHLKRVSRSLALVPT
                     RLGEYEQILLWERSAWDAFRKVMEELYTPMQLGDAWSLSKGFNSYDHVVTPHLYESPQ
                     TIRQRPLTHGPVFLAARRQVHLATRWKAPIWLAEGFAAYGDHAVHKANRWWSVYDPGK
                     TPAVGDWLADARRLTKAGSVQPWDKLLRRELRDWEPDDYYQTLGIVAFLLEAEPGKFL
                     ALLRKLAAGDDQVAALEDAYRQPLAELDRRASTWLVARRS"
     gene            8149..8982
                     /locus_tag="VFB80_15580"
     CDS             8149..8982
                     /locus_tag="VFB80_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35249.1"
                     /translation="MRFAICNETFQDWPFDKAFRFAAELGYSGLEIAPFTINVNAYEI
                     SPARRAEVRRQADDAGLEIIGLHWLLAKTTGYYLTTPDDAVRHKTSDYLGELARLCRD
                     LGGNVMVLGSPMQRNLLPGVSHEQGMELAADCLRRAMPTLEQCQVTLAVEPLGPKDGN
                     FLLTAAAGRQLCEMVGSPHCRLHLDCKAMASESKPIPQLLRENASLLEHFHANDPNMR
                     GPGMGELDFRPIFQALSDIDYRGWVSVEVFDYTPGVEALARESIEYLEDCEADLAAPD
                     R"
     gene            9196..9510
                     /locus_tag="VFB80_15585"
     CDS             9196..9510
                     /locus_tag="VFB80_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35250.1"
                     /translation="MANKAHVAVVRRLNERYGNGADNSNGFDVHIGDLVIEVETSATL
                     HDGFQKLQGSAGRRYIAVTNQEAIDDAMALTKESPIGVMNPWGDILRDAVPPEVSPAK
                     SA"
     gene            9649..10932
                     /locus_tag="VFB80_15590"
     CDS             9649..10932
                     /locus_tag="VFB80_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35251.1"
                     /translation="MSLLGASASGWFPLLAKDLAADPKRRRHCILLWMTGGPSQTDTF
                     DMKPGHANGGEFKEVATKAPGLRFSEHLPKLGEMAEHLAVVRSLSTKEGDHGRGTYVV
                     RTGQKPQGPVQYPAIGSSLSKALANEDEAVPHYVSISPYRAFNQAAFSPGFLGPRYAA
                     LTVGATDGVQQAPAAAGGYAQLQVDDLTPGVAKSQFAGRVDLWRALESRFVANHRGPS
                     PAAHQTVYERALKLMDSAAARAFDLSEESSAVRDAYGRGRFGQGCLMARRLIESGVSF
                     VEVTLGNVGDNMFGWDTHQNNFTAVKALSAELDNGWGTLMKELAERGLLDSTTILWIG
                     EFGRTPAINMQAGRDHFPAAWSCVFAGGGIKGGQAFGKTSEGGEEVKEGKVDVPEILA
                     TLCAAVGVDPEEKNVSEQGRPIKIADGKPIRDILA"
BASE COUNT         1881 a         3648 c         3730 g         1735 t
ORIGIN      
        1 ccgggcttta acggcctgac gcaggaggtc ccggcctcgg gcatcggccg caacgactat
       61 ccgggcaatt ttgccgagtt cctcaaacag cggcccaagg agcagccgtt ctgcttctgg
      121 atgggcttca cggagccgca ccgggcgtac gaattcgact ccggcgtgcg gctgggcaag
      181 aagctggccg acgtgaaggt cccggcgttt ctgcccgaca ccgatatcgt ccgccgcgac
      241 ctggccgact atgccatcga agtcgaatgg ggcgatcagc agattggccg cacgctcaga
      301 atcctggagg acgccggcga gctgaatcgt acgctggtcg tggtcacgtc cgaccacggc
      361 atgccattcc cgtacgtcaa ggggcagatt cacgaggacg cgttccacct gccgctggcg
      421 atgcggtggg ctggcggcat caagccgggc cgcgttgtcg aggatttcgt caacgttcgc
      481 gacctcgcgc cgacctactt ggagctggcc ggcctggccg tgcatccgca aatgaccggc
      541 aagagtctcg cggcgatcct gcggtccgac aagtcgggct ggatcgagaa ccgcgacgtg
      601 atgctcgtcg gcaaggaacg gcacgacatt ggccggccga acgacctggg ctatcccgtg
      661 cgggcgatcc gcacgaagga ctacctctac gtccacaatt tccatcccga gcgctggccc
      721 gccggcaatc cagagacgga ctatcccaac tgcgacgccg gcccgtcgaa ggaagtaatc
      781 aaggtcctgg gcggccactt cctcgagatt tcgttcggca agcgcctgcc cgacgagctg
      841 taccgcctct cggacgatcc ggagtgcgtc cgcaacctgg ccaacgatct ggccttcaag
      901 gaagtcgccg acgagctccg ctacaagatg tggacgatgc tcaagaacga aggcgatccg
      961 cgggccgccg ggcagacgga aatcttcgac acctacaaat acacggccgc ccgcggcaag
     1021 ggctacgaaa cctggctgca gcagcaggaa gccggcccgc tcgccgagct gaaggccaaa
     1081 ttggaagaag ccgccaagcg tccgcccgcc aagggcaagg ccaagaaagc caagtcgagc
     1141 aactgactca aagccgcgac cagtgatcgc ggtctcctcg caatgaagcg gtcaatcttc
     1201 ttgccgctgg tggccgcggc catggtctcg gcccacctgt cagcggccga cgtgctgccg
     1261 cactggattt ggaccaacga gccgggcagc cagcgggcga cgtttcagca caacttcacc
     1321 gcgccctcgg ccgtgcaatc ggcgagcttg cgcgctgtgg ccgatttctg cctcgcgggc
     1381 atttggatca acggccggcc ggttggagcg atcgagccgt acgggccggc gctcgacata
     1441 gatgtgacgg cagcgatccg cgccggccag aacgagatcg caatcaacgc cggcgcggta
     1501 ccgggccccg cagcgatcgc cctccggctg acgatcgtag ccgcggacgg cacgcggcgt
     1561 tcgatcgtga ccgacgaaac gtggcgcggc gcgaagtctg cgggcgaggc actgccggcg
     1621 ctgtttggca ttggccgccg gccgccgacg atcgacccgt tcgacaacta cgagcagtgg
     1681 cggcaggcga tcggtgcgtc tgcggcgagc gccgccgcgt tctggaccgc acccagtttc
     1741 gagatcacgc tggtccgcgc ggccccgtcg gaggaaggct cttgggtcag cctggcgttc
     1801 gacccgcagg gccgggcaac gatcgcccgc gaagaaaaag gcctgcttag gatgacgctg
     1861 gccgacgatc gccgcagcgt ggcaaaggtc gagacgatcg acgacacgct gctggaatgt
     1921 cgcgggctgc tctacgccca cggcgcgctc tacgccagcg ccaacaactc caaggggctc
     1981 taccgcctgc gcgacgccga cggcgacggg cggttcgacg agacgaagct ccttcgcgaa
     2041 ttgagcggca gcgttggcca cggccgcaac gacctggccc tcggccccga cggctcgatc
     2101 tactggatca gcggcgactc ggtcgatctg cccagggaga acgtcgtcga tcgcacgtcg
     2161 cccttccgtg aggcccgccg cgggcaaacg acccgcgagg gcttcgtcct ccgcaccgac
     2221 cgtgatggcg ccaagtggga attgatcgcc gcgggcctcc gcaatccgtt cggcattgcc
     2281 ctgaacgagc acggcgacgc tttcacctac gacgccgacg ccgaattcga catggggtcg
     2341 ccctggtacc ggccgacgcg cgtcgtgcag ctggcggccg ggtccgattt cggctggcgc
     2401 ggcgtcaccg gcaagtggcc tccatacttt cccgaccacg ccgacaacgc gctccccgcg
     2461 ctcgacattg gccggggatc gccgacggcg gtaatgtttg gcacggacct gaaattcccc
     2521 gccgactaca agcgcagcct gctgatcctc gattggacgt acggccggat tctcgccgtc
     2581 cacctgctgc cgcggggtgc gggctatcgc ggcgcggccg agacgctgct caagggccgg
     2641 ccgctcaacg tcaccgacct ggccgccggt cccgacgggg cgctgtatct agtcaccggc
     2701 ggacgcaaga cccagtccgc cctgtatcgg ctcgccttca ccggggcaga gcggcaagat
     2761 tcgccgccgt ccccccacga gaaggcctgc caggaccatg cccaagcgtc ccgtgccgtg
     2821 cggaagcagc tccagcagct ccatcagcca agcgccgccg cgctcgacgc cgcctgggcc
     2881 tatctcaacg accacgatcc gcggctgcga tacgccgcga tggttgcgac cgagcatcag
     2941 cttgtggatc agtggcgcga gcgggccctc aaggaaaaac ggccgatggc cgcgctcacg
     3001 gcgctcgtcg ccttggcccg cggcggcgac gcggcggccc tcgaggctct gctcgagctg
     3061 ccggcaggac aattcaatgt cggccaactg cttttgctcg tgcagagcta cctcctgctg
     3121 aacgacgccg ccccggcaga agtggccagc cgcaaacagc ggatcatcgc gcagctagat
     3181 ccgctcttcc cgcatccggc ggctgcggcg tcgcacgtca gcccggctgg aacggccgcg
     3241 agcctcaatc gcgatctggc tcgcctgctg gcggcgctcg acgcgccagg ccttgtcgaa
     3301 cgagtggcaa agtcgctcct ggccagcccc gcccaggaaa accggttgca cggcctcttc
     3361 gtgctccgca acgtgaagag cggctggaca agcgacacgc ggcgggcgta cttcacggca
     3421 ctcaacgacg gcgcgaagta tgtcgcgggg gaaggaatgc ccaagttcct cgcgcaaatc
     3481 cgcgaacagg ccgtgctggc gctcacggac gctgagcgcc gcgagctggc cgacctgctg
     3541 gaggcaaagg cgatcgacga cgagccgttg cctcccgcgc ggccgctggt caagcgctgg
     3601 gaggcgggcg acttcgagaa gctgctggcc gatccggatc gccgcggcga tgccgagcgc
     3661 ggctcagtcg tctttcgcga ggccctatgc gcccgctgcc accgcgcggg tgctcgcggc
     3721 ccggcggtcg gaccggacct gacgcacgtg gccggccgct tcagcccgcg ggacctgctc
     3781 gactcgatcc tcacgcccaa ccgcgtcgtc gccgagaact accgcggcgt gcaggtccgc
     3841 acgaccgacg gccgcacgat caccggccgc gtcctgctcg aaggcgacta ccgctccgaa
     3901 aagctccgcc tctccacgca gccgctggcc cccgcggagt tcgtcgagct cgacaaaaag
     3961 cagatcgagg aggttcgcga gtcgcccatt tcgcccatgc ccgaggggct gctcgacggc
     4021 ttccgcgaga gcgacgtgct cgacctgctg accttcttga cgcgcgcgac tcgctgagca
     4081 gacaatttgt cttaatccta tcgcgtgagg gtccgatggc tgcaaatcgt gccgggcgcc
     4141 tgacaattgg gctgtacttg ctcgcgctgg cggctgcgcg agtcgcgccg gcccaaacgc
     4201 ccgcggagcc tgtcgatttc cttgcgccgg ccgaggtcat cgtaggtccg acgcggctgg
     4261 cctggggcgg ggtgctgtcg cccgatgagt cgtggtttgc cgttggttac ggccactgga
     4321 gcaacgagct gggccgcgtc tgcgtcttcg accgggccag cggcaaattg cgctggagcg
     4381 ccgaggagcc gcgcggagtg cgcggcctgg ccgtctcgcc cgacggcgcg ctcgtggcca
     4441 gcggcaactt cgccggcgag atccgcctcc gcgacgcggc gagcggcgcg gtgcggcaaa
     4501 cgctcctcga gacgggcggc tcgatcgaaa agctctcgtt cgcccacggc agccggcggc
     4561 tggccacggg cagcaacggc aatgccgttc gcgtttggga cctcgccacg ggccagatcg
     4621 agcggacgct gatcggtcac acgggacggg cgtattggat cgagttctcg ccggacgaca
     4681 agctgcttgt cactgccagc caggaccgct cggtgcggat ttgggacgcg gccaacggcc
     4741 tcctgaagca cacgctcgcg catccgggcg aagtatccgc cgccgtgttc ctgccggacg
     4801 gcttgcaggt ggcgacggtc tgccacgacg ggctgatccg cctgttcgac atccagtcgg
     4861 gcgcgttgac gatgacgctc caggatgcgg gccagcccct cgcggccaag tacgcccttg
     4921 ccgtctcggc cgacggcacg acgctggccg cgggcaacag cggccagatt caaatctggg
     4981 acctcgtggg cggcaagctg cgggccacgc tcgccggcca ccgcggcctg gtcttcggca
     5041 tcagcctgtc caaggacggc cggacgctgg tcagcaccgg ctgggacgcg ctggccaagg
     5101 tctgggacgt agcgggcgag cgcgagctgc ggtcgattcc gctcccggcg tcccatcacg
     5161 attcgcccgg tccgatcgtc ggtctggcgc tgtcgcccga cggcgcgctg gccgctgccg
     5221 tgctcaccga ccagacggtt cagctccgcg accgcaagag cggcaaggtc gtgcggacgc
     5281 tgacgggcca cagcgacgtt gtgctggcgg cggcctttgc cgacaacggc aagtggctgg
     5341 ccacgagcgc cgccaatggc gaggtccgca tctggaacgt cgccagcggc caattgcaca
     5401 agacgctcag cgggcacaag agcgcagtct atgccgccag ctggtccgcc ggcggcaagc
     5461 tgctggctac ggccggcgac gacaagacgg tgcggctgtg gaatggcgat tcgttcgcgc
     5521 cgctggccac gttgagcgga cacgccgacg cggtgcgctc gctggcgttt gccccggacg
     5581 ccgctcggct ctattcgggc agttccgacg gcacggcgat catttgggat gtcgccaagc
     5641 gcgccgccga aggcacgctc gcgggccatc cggccgcgat ccgcgcgatt gcgacctcga
     5701 gcgacggcgt cagcgtcgcg acgggcggcg aggaaggcgg ggtcaccgtc tggaacgtcg
     5761 ccacgcgcca gccgcggctg aagctcgccg gccatccgat gggcggcgtc ctgtcgctgg
     5821 ctttttcgcc gcgcggccag acgctggcca gcagcggcgc cgacaagaat attcggctct
     5881 gggatccggc caacggtcag atgcgcaagc tgttcggagc gcacgccgcg gaagtgaccg
     5941 gcgtggcctt cctgccgcag gccgcgggac tggtcagcgc cagcaccgac ggttcgctcc
     6001 gactgtggaa agccgacgag ccgcggacgc agcccgtggt ctcgctgccg gcccacgcgc
     6061 ccgaggctta ctgcgccaag ttctcgcccg acggccagtg gctagtcacg ggcggcaaga
     6121 acagcctgat cgccctccgc gatcccgtga cgggcgaggt caaaacgacg ctcaccggcc
     6181 acagcggcat catctacgac gtcgccttct cgcccgacta cttgctgctg gccagcgccg
     6241 gatcggacgg caccgtgcgc ctctggtcgc tcaaccgggc cgtggagatc atgaagtaca
     6301 acggctacgg ggcgaagctg gcgggcatcc gctccgtcgc cttttcgccg gacggccgca
     6361 cgctggccag cggcgcgggg gacggcacgc tgaaactgtt cgacgtcgat tcgggcgtcg
     6421 tccgcaagtc gctgccgagc cagacgctgt ccgtcaacag catccgcttc acgcccgaca
     6481 gcacgctcat gatcaccgcc accggcgact ggcagcagcc gaacgttgcc ggcgaattgc
     6541 gggtgtggga agtcgccagc ggccaggagt tcgcaaaact gccggggcat acggtggaaa
     6601 tcaagcggat cgacctcagc cgcgacggca agcggctcgt ctcgtccggc tccgaccgca
     6661 cgctgatggt ctgggacctc acccagcgga aagtgatcgg cacggtgaag accgagacgg
     6721 tgggaacagc gattgcgctg ttgccggacg gccagcggct ggcgatgggg gacggccgcg
     6781 gcggcgtctg gctgtgggac ctgacggcgg gcaagctgct tacccgctat gcgggccaca
     6841 acaaggttat tccgggcatc ggggtcagcc ccgatggaaa gctgatcgcc tcggcttcgc
     6901 aggacggcac gctcaagctg tggccggtac ggtagcgatc tacgagcggc gggcgacgag
     6961 ccacgtactg gcacggcggt cgagctcggc cagcggctgg cgataggcgt cttccagcgc
     7021 cgcgacctgg tcgtcgccgg cggccagctt gcgcaagagc gcgaggaact tgcccggctc
     7081 ggcttcgagc aggaacgcga caatgcccag cgtctggtaa tagtcatcgg gctcccagtc
     7141 gcgcagctcg cggcggagga gcttgtccca cggctgcacg ctgccggcct tggtgaggcg
     7201 gcgggcatcg gccagccagt cgccgaccgc cggcgttttg cccgggtcat aaaccgacca
     7261 ccagcggttg gccttgtgaa cggcgtgatc accgtaggcg gcaaagccct cggccagcca
     7321 gatcggcgct ttccaacgcg tggcgaggtg gacctggcgg cgggcggcga ggaacaccgg
     7381 gccgtgcgtc agcggccgct ggcggatcgt ctgcggcgac tcgtacagat ggggcgtgac
     7441 gacatgatcg tagctgttga atcctttgga cagcgaccag gcatcgccca gttgcatcgg
     7501 cgtgtacagc tcctccatca ccttgcggaa ggcatcccac gccgagcgct cccagagcag
     7561 gatctgctcg tactcgccca gccgcgtcgg cacgagggcc agcgaccggc tgacgcgctt
     7621 caggtgtagc gtgaggcttt cgaccgcctg gccggcactc ttggcctgcg cgaaggtgaa
     7681 ctgcgtggtc agggcagcgt gccgcgtcat gaccaggacc aggcggccgc cgatccgctc
     7741 tttccattcg gcgtgcttcg actgggcccg ctcgaaccgc cccttggcgg ctgcgacgag
     7801 ctccgccgaa tctccgccag cccggcaaac cgggcagtgt tgctcgccga ctgcggcttc
     7861 cggcttgggt gcgggctggc catcgaccca gacgaacggc ttggcgtcgg gaattggcac
     7921 caggccgacg ccgccgcact gcgggcagtc gtcagcgccg cgctcctggg ccagcgcgct
     7981 tgtgccgaga agagacagtc ccgaggccgc caggaacatc cggcgcgaat gactaggcgg
     8041 acagcgattg cacgtgcggc ccatgatccg atcattgtag cgcactggga acagagaatg
     8101 gatatcctga tgtgagttgc ctgtcatccg cgaatgaaat ccaccaccat gcgttttgcc
     8161 atttgcaacg agacgtttca ggattggccg ttcgacaagg cgtttcgctt tgcggcggag
     8221 ctgggctatt ccggcctgga gatcgcgccc tttacgatca acgtcaatgc gtacgagatt
     8281 tcgccggcgc ggcgggcgga agttcgccgc caggctgacg acgccggcct ggaaatcatc
     8341 ggcctgcact ggctgctggc aaagacgacc ggctactacc tgaccactcc cgacgacgcc
     8401 gtgcggcaca aaacgtccga ctatctcggc gagctggcgc gtctctgccg cgacctgggc
     8461 ggcaacgtga tggtgctggg ctcacccatg cagcggaacc tgctgcccgg cgtctcgcac
     8521 gaacagggaa tggaactcgc ggctgactgc ctgcggcggg cgatgcccac gctcgagcag
     8581 tgccaggtga cgctggccgt ggagccgctg gggccgaagg acggcaattt cctgctgacc
     8641 gcagccgcgg ggcggcagct atgcgagatg gtcggctcgc cgcattgccg gctgcacctg
     8701 gattgcaagg cgatggcctc cgagagcaag ccgatcccgc agctgttgcg ggaaaatgcc
     8761 agcctgctgg agcattttca cgccaatgac ccgaacatgc gtggtccggg catgggcgag
     8821 ctcgactttc ggccgatctt tcaggctctt tcagacattg actatcgtgg atgggtttcg
     8881 gtggaagtat ttgactatac ccccggtgtg gaagctctgg cccgcgagag tattgaatac
     8941 ctggaagatt gcgaagcgga tctagcagcg cccgatcgct gataaactga ttcgagcaaa
     9001 ggagttaggt gctttgggca gagttttact cgtaccgttt gcggtgaaca attcttgcgc
     9061 cgaagagaaa atgcgtgcca aaaaccgcat gcgtcggaaa ttttgcggtt agccgcctat
     9121 gagaggtagt ggcgggactc gcggccaatg ccgcaggggg gtctcagccg catccctccc
     9181 taggcggtgc aagtcatggc gaataaggca catgtggcgg tcgtccggcg gttgaacgag
     9241 cggtatggca acggggccga caattcaaat gggttcgacg tgcatatcgg cgacctggtg
     9301 attgaggtag aaacttccgc cacgctgcac gacggctttc aaaagcttca gggatcggcc
     9361 ggccggcgat acattgccgt cacgaaccag gaagccatcg acgacgccat ggccctcacc
     9421 aaggaatcac cgatcggcgt catgaatccg tggggcgaca ttctccgcga cgcggtgccg
     9481 cccgaagtat cgccggcgaa gtcggcctag cggatttgca cgacgcctcg gtttcggcga
     9541 caattgtggt tgctcttggg gaccggtcgt tcgacatcga ggtgtccaat gcgctgtatt
     9601 cctaacagcg ggcttcgcgc tcgccgtcaa tggctgcaag ccgcttcgtt gagtttattg
     9661 ggggctagtg ccagcggctg gttcccgctc ttggccaagg acctggcggc cgatcccaag
     9721 cggcggcggc actgcatcct gttgtggatg acgggcgggc cgtcgcagac cgacactttc
     9781 gacatgaagc cggggcacgc caacggcggc gaattcaagg aagtcgccac caaggcgccg
     9841 ggcctgcggt ttagcgagca cctgccgaag ctgggcgaga tggccgagca cctggcggtg
     9901 gtccgcagcc tgagcacgaa ggaaggcgat cacggccgcg gaacgtatgt cgtccgcacc
     9961 ggccagaagc cgcaagggcc ggtgcagtat ccggcgatcg gctcgtcgct gtcgaaggcg
    10021 ctggccaacg aagacgaagc ggtgccgcac tatgtgagca tttcgcccta ccgggcgttc
    10081 aaccaggcag ccttttcgcc gggcttcctt ggcccccgct atgccgccct gacggtgggc
    10141 gcgaccgacg gcgtccagca agcaccggcc gccgccggcg gatatgccca gctgcaagtc
    10201 gatgacctga cgccgggcgt cgcgaagtcg cagttcgccg gccgcgtcga tctgtggcgg
    10261 gcgctggagt cgcggttcgt ggccaatcac cgcgggccgt cgccggccgc tcatcagacg
    10321 gtatatgagc gggcgctcaa gctgatggat tcggcggccg cgcgggcgtt cgacttgagc
    10381 gaggagtcct cggctgtccg cgacgcctat ggccgcggac ggttcggcca gggctgcctg
    10441 atggctcgcc gcctgatcga gtcgggagtg tcgttcgtcg aggtcacgct aggcaacgtc
    10501 ggcgacaaca tgtttggctg ggacacgcac cagaacaact tcaccgccgt caaggcgctc
    10561 tcggccgagc tcgacaacgg ctggggtacg ctgatgaagg aactcgccga gcgcgggctt
    10621 ttggattcga cgacgatcct ctggatcggt gagtttggcc gcacgccggc gatcaacatg
    10681 caagccggcc gcgatcactt ccccgccgcg tggagctgcg tcttcgccgg cggcggcatc
    10741 aagggcggcc aagcctttgg caagaccagc gagggcggcg aggaggtcaa agagggcaag
    10801 gtcgatgtgc ccgaaatcct ggccacgctt tgcgccgccg tcggcgtcga tcccgaggag
    10861 aaaaacgtct ccgagcaagg ccggccgatc aagatcgcgg atggaaagcc gattcgagac
    10921 attctggcct aggtccgaag cagaggaatg gaacagaagg cagcaaagga agcaaagcag
    10981 cggatgcgac tgca
//
LOCUS       DASRJB010000590         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_456062, whole genome shotgun sequence.
ACCESSION   DASRJB010000590 DASRJB010000000
VERSION     DASRJB010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456062"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFB80_15595"
     CDS             <1..733
                     /locus_tag="VFB80_15595"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZN35252.1"
                     /translation="VQLVSTTDREAVGQFLRMPEYIDVAIPRGGESLIRRVAAEAQMP
                     VIKHFDGNCHVYVDAAADLEMAREILVNSKCQRMGVCNACESLLVHAAVADAFLPRIG
                     AALGQRGIEMRADELSRNLLGVPGVKPATEDDYAAEFLGPIISVKVVESLEAAIDHIN
                     RYGSQHTDAIVTRDLAAAREFAARVDSSAVMINASTRFNDGYEFGLGAEIGISTDKFH
                     ARGPCGLKELTTYKYVVFGEGQIRE"
     gene            821..1855
                     /gene="fliM"
                     /locus_tag="VFB80_15600"
     CDS             821..1855
                     /gene="fliM"
                     /locus_tag="VFB80_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HZN35253.1"
                     /translation="MTDGILSQSEVESLLSTMEAQSPRAATPAAAVPAPRAALKPREK
                     ITTYDFKRPERVGKEQMRALQSLHEGLGRNFGAALSALLRSIVEVKLTSVDQLTYSEF
                     VFSLENPTCFNLLKAEPLEGNLILDINPSILYPIIDRLLGGGKEAGPIARRPLTEIEL
                     RLVSRITGLFLQELKHAWQGLLSLKLSVDRVESNPQLVQIVPPNEVVILVSFELTLGD
                     VRGMINFCIPFNSIERVSSKLASNSWASYGKTASTGQTQAAIGRRLDEAKLDLVVTLA
                     ESHISTGDLIGLRVGDIITTDKDVRLPLDVAVQGTTKFHATAGAFKGCKAIRVETPPL
                     PPQQPAKLPG"
     gene            1864..2424
                     /locus_tag="VFB80_15605"
     CDS             1864..2424
                     /locus_tag="VFB80_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35254.1"
                     /translation="MYRLAFALVVVLLLWVQSLFDTTTHTVSGSHHVFAYGWLAWFFQ
                     GLFIVALLGFAWLAWRWMQDRVVAGIMLAGVPLFCLVVMPQTACERVELTDELLIHRR
                     EPPHTKYNFDIPLSEIASATAVSQETGSFGTEHMFGYRFVTKDGRQYDLPLSTTLTAA
                     HEAIDQELSARKIPLNIELRERPPAR"
     gene            2806..3450
                     /locus_tag="VFB80_15610"
     CDS             2806..3450
                     /locus_tag="VFB80_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZN35255.1"
                     /translation="MAAGPAAAASAEGSGETRPLHRHPTLFGMLVRSNNMRRRAGLLP
                     HRMNPALTKAAQDHAAYMARTGQFSHYANGGYQYRASKYGFRGGVLENIAMNGGGLDA
                     AFSMWQASGAHYASIVSRTTDAGFGYAVGPGGAVYYVGVYGTPPAGDEVGETEESIAA
                     YFEEEKKAAEKLAAEKAAAENDPNVKPASATTSTEQKPANEVKPAAATQSASKN"
     gene            complement(3571..>4223)
                     /locus_tag="VFB80_15615"
     CDS             complement(3571..>4223)
                     /locus_tag="VFB80_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35256.1"
                     /translation="GLACLLAAGCGVPSSASHAPTDVPVAAAAQVGDAPTPTEQSPTV
                     EPAEPAAAPAEAPAPAAAAPPASDDAPKPPAPKAQRLPADRRPRRPGEAEKITFEDLN
                     LGMQADVAYRPFMLTDRVKELEGQRVSILGYMHEGQLSTNSVKKFVLLKNTQCKFGPG
                     GQADHLGMIYLKEGTAAKLTNQVVKVEGKLKIEPVNVDGITWSVYDVEDATVEVVK"
BASE COUNT          763 a         1342 c         1353 g          765 t
ORIGIN      
        1 agtccagttg gtgagcacga ccgatcgcga ggccgtcggc cagttcctgc ggatgcccga
       61 gtacatcgac gtggcgatcc cccgcggggg cgagagcctg attcgccgcg tggccgccga
      121 ggctcagatg ccggtcatca agcacttcga cggcaactgc cacgtgtacg tcgacgcggc
      181 ggccgatctc gagatggccc gagagatcct cgtgaactcg aagtgccagc ggatgggcgt
      241 ctgcaacgcc tgcgagtcgc tgctggtgca cgccgccgtg gccgatgcgt tcctgccgcg
      301 aatcggcgcg gccctgggcc agcggggcat cgaaatgcga gccgacgagc tctcccgcaa
      361 cctgctcggc gtgcccggcg tgaagccggc gacggaggac gactacgccg ccgagttttt
      421 ggggccgatc atctccgtga aggtggttga gagcctcgag gcggcgatcg atcacatcaa
      481 ccgctatggc tcgcagcata ccgacgccat cgtcacccgc gacctggccg cagcccggga
      541 gttcgccgcc cgcgtcgata gctcggcggt gatgatcaat gccagcacgc gattcaacga
      601 cggctacgag ttcgggctgg gtgccgaaat cggcatcagc accgataaat ttcacgcccg
      661 cggcccctgc gggctgaagg aactgacgac atataagtat gtggtgtttg gggaagggca
      721 aatcagggag tgagaccggt cagccggtga gtcggtaagt cggttggtca gtaatctgtc
      781 caccgatcaa ccgaccaccg accaaccgac caaccggcct atgaccgacg gaatcctcag
      841 ccagtccgaa gtcgagagcc tgcttagcac gatggaggct cagtcgccgc gcgccgcaac
      901 tccggccgcg gccgtgccgg ctccgcgggc ggcgctcaag ccgcgggaaa agatcacgac
      961 ctacgacttc aagcggccgg agcgcgtcgg caaggagcag atgcgggccc tgcagtcgct
     1021 gcacgagggg ttgggccgca atttcggcgc ggccctctcg gccctgctcc gctcgatcgt
     1081 cgaggtcaag ctgaccagcg tcgatcaact cacctacagc gagttcgtgt tcagcctgga
     1141 gaacccgacc tgtttcaatt tgctcaaggc cgagccgctg gaaggcaacc tgatcctcga
     1201 tatcaatcct tcaatcctct atccgatcat cgatcgcttg ctgggcggcg gcaaggaggc
     1261 cggcccgatc gcccgccgcc cgctgaccga gatcgagctg cggctcgtct cgcggatcac
     1321 ggggctgttc ctgcaggagc tgaaacacgc ctggcagggc ctcttgtcgc tgaagctctc
     1381 ggtggatcgc gtcgaaagca acccgcagct ggtgcagatc gtgccgccga acgaggtcgt
     1441 aatcctcgtc agcttcgagc tgacgctggg ggacgtccgc ggaatgatca acttctgcat
     1501 cccgttcaac tcgatcgagc gagtcagcag caagctggcc agcaacagct gggccagcta
     1561 cggcaagacc gcctccaccg gccagacgca ggccgcgatc ggccggcggc tggacgaggc
     1621 gaagctggac ctcgtcgtca cgctcgccga gtcgcacatc tcgaccggcg acttgatcgg
     1681 actgcgcgtg ggagacatca ttacgaccga caaggacgtc cgcctgccgc tggatgtcgc
     1741 cgtgcaggga acgaccaagt tccacgcgac cgccggcgcg ttcaagggtt gcaaggccat
     1801 tcgcgtcgaa acgccgccgc tgccgccgca gcaaccggcg aagctgcccg gctaagttca
     1861 gccatgtatc gactggcatt tgccctggtc gtggtcctgc tgttgtgggt gcagtcgctg
     1921 ttcgacacga ctacgcacac ggttagcggc agccaccacg tgttcgccta cggctggctg
     1981 gcgtggtttt tccaagggct gttcattgtc gcgctgctgg gatttgcctg gctggcgtgg
     2041 cggtggatgc aggaccgggt tgtggccgga atcatgctgg ctggagtgcc gctgttttgc
     2101 ctggtcgtga tgccgcaaac ggcctgcgag cgcgtggagc tgacggacga gttgctgatt
     2161 catcggcggg agccgccaca cacgaagtac aacttcgaca ttccgctctc ggagatcgcc
     2221 tctgccacgg ctgtgagcca ggaaaccggc tcatttggca ccgaacatat gttcggctac
     2281 cgatttgtta cgaaggatgg ccggcaatat gacttgccgc tcagcacaac actgactgcc
     2341 gcacacgaag ccatcgatca agaattatct gcccgcaaga ttccattgaa tatcgagctt
     2401 cgcgagcgac cgccggctcg atgagtcgct cgccatcgac agccatcgac aggctatcca
     2461 ccgcggcagc tagcacaatc ggtctatgac cgttgtaaaa cgccctagga cagctcctac
     2521 ccaagctttc tattctgctt aactttctag ttttaccatt ttttggtgtt taacatccac
     2581 catatggggt ggagtctgtg gtgagttggg gttttggggg gattgccagc cgcttggccg
     2641 tttgaccata acggccccgc aaactcaggc cagcttgcct gggttcacgc ctttggttcc
     2701 ccaccgtttc gttcccacct gtttgcacgg aggagaagta tgaggagagc acttggattg
     2761 gcggccgttc tggccgcgtc ggttgccttg gctgcagaac cgcccatggc agcgggaccc
     2821 gcggctgccg cttccgccga gggttcgggc gaaacccgac cgctgcaccg gcacccaacc
     2881 ctgttcggca tgctggttcg cagcaacaac atgcggcggc gggcgggcct tttgccgcac
     2941 cggatgaacc cagccctgac gaaagccgct caggaccacg cggcatacat ggcgcggacc
     3001 ggccagttca gccattacgc caacggcggg tatcagtacc gggccagcaa gtacggcttc
     3061 cggggtggcg tcctcgagaa catcgccatg aatggcggtg gactcgacgc ggcgttcagc
     3121 atgtggcagg cgagcggggc ccattacgcc tcgatcgtca gccgaactac cgatgcgggc
     3181 ttcggttacg ccgtgggccc cggcggtgcg gtctattatg tcggcgtcta cggcacgccg
     3241 cccgccggcg acgaggtcgg cgagacggaa gagtcgatcg cggcttactt cgaggaagag
     3301 aagaaggcgg cggagaagct cgcggcggaa aaggccgcgg cggagaatga ccccaacgtc
     3361 aagccggcct ctgcgaccac tagcacggag cagaagccag ccaacgaggt caagcccgcc
     3421 gcagccacgc aatccgcttc gaagaactag ctcatcggac ccggccagcc gcatcgcggc
     3481 cggcagcggt ccccaagtcc tcttcaattg tagagggcga gtcaaatcgc agtcgcggaa
     3541 ggcggcagga tttcgccttc cgcggctgcc tcatttgaca acttccacgg tcgcatcttc
     3601 gacgtcgtag accgaccagg tgatgccgtc cacgttgacc ggctcgattt tcagctttcc
     3661 ttcgaccttg accacctggt tcgtcaattt ggccgccgtg ccttccttga ggtagatcat
     3721 tcccaagtgg tcggcctggc cgccggggcc aaatttgcac tgcgtgtttt tgagcaggac
     3781 gaacttcttg acggaattcg tcgacagctg cccttcgtgc atatagccca ggatcgaaac
     3841 ccgctggccc tccagttcct tcacgcggtc agtgagcatg aacggccggt aggcgacgtc
     3901 ggcctgcatg cccagattga gatcttcgaa cgtgatcttc tcggcctccc cgggccgccg
     3961 tggccggcgg tcggctggaa gccgctgggc cttcggcgcg ggcggcttgg gagcgtcgtc
     4021 actggctggt ggggcggcgg ccgcaggtgc tggggcctcc gcgggcgcgg cagccggctc
     4081 agctggttcg acagttgggg actgctccgt gggcgtagga gcatcgccaa cttgcgcggc
     4141 agcggccacc ggaacgtccg tgggtgcatg gctcgccgac gacggcactc cacaccccgc
     4201 ggccagcaga caggcgagtc cga
//
LOCUS       DASRJB010000591         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1582447, whole genome shotgun sequence.
ACCESSION   DASRJB010000591 DASRJB010000000
VERSION     DASRJB010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582447"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..387
                     /locus_tag="VFB80_15620"
     CDS             178..387
                     /locus_tag="VFB80_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35257.1"
                     /translation="MRYRLRTLLIALAVGPPLLARPIAWLWEHPAVFLGLVFAAIFAI
                     FWVSCGIIWLKVVQLFVHFSEVPRN"
     gene            397..696
                     /locus_tag="VFB80_15625"
     CDS             397..696
                     /locus_tag="VFB80_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35258.1"
                     /translation="MVRFSIRDVLWLTALVALAVGWWLDRREVQRLGEARAAAIRSHA
                     EALQAALRNAQLYQSLTSSTTGTVVSSGAPGSSYIDYGPITPDVDWSLADKPLPP"
     gene            702..932
                     /locus_tag="VFB80_15630"
     CDS             702..932
                     /locus_tag="VFB80_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35259.1"
                     /translation="MTLRYKLRTLLILLAVLPPLLGVAWLKYAAWKAKQDELQRRQGW
                     VIEVRTYVQETGLPYTLEDVQGTVPPSEPPKP"
     gene            948..1187
                     /locus_tag="VFB80_15635"
     CDS             948..1187
                     /locus_tag="VFB80_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35260.1"
                     /translation="MFRYKLRTLLILLAILPPLLGVAWLKYAAWKAEQARRAELAKMQ
                     QLEIIFDEIQPVYVQQLSSFFDPPEPLPLADPPSN"
     gene            1379..2428
                     /locus_tag="VFB80_15640"
     CDS             1379..2428
                     /locus_tag="VFB80_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN35261.1"
                     /translation="MTPLMNRRRSLQTLGALASGAVVAGLCRRAEAAKADPKVEAAIE
                     KGLKWVAKTQSSLGHWTAGGYPTAMTALAGTALIGSGSTTIQGPYAKHIRKTVDYLLG
                     KLRPNGLIGDPASDNRYTYGHGFSMLFLSQVLGEEEDEERRDELVTALEKACDFSGKA
                     QTPSGGWGYISAKDGNNFDEGSTTITQVQGLRGCRNGGIVVPGDVINKAKEYIYGCKN
                     PDGGISYSSRNRGSSRPAITAAALAALYNAGDYDSKHVPEMLEYCKKTLQNLSEGPNS
                     FGHWHYTYLYFSQVMYRQGNKEWEPFRDKLYSHIVKEQLNDGSWQGNFGPIYPTSCNL
                     IMLQLDKAYLPIYQR"
     gene            complement(2486..3307)
                     /locus_tag="VFB80_15645"
     CDS             complement(2486..3307)
                     /locus_tag="VFB80_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZN35262.1"
                     /translation="MSRRVHDDDAGQGPRPAALLGGIYSNYLALEAALADIRRRGVRD
                     VYCLGDLGAFGPHPDRAIELLREHGVACVRGNYDDSIGRELADCQCGYTDPRDNHFAQ
                     ISYDYTLANTSPANRAWLRELPAEIRLELGGRRVLLCHGGPRRMNEFLWESTSSTHFL
                     SALCEQYAADVICTTHTGLKWQRELPGGKLFLNVGVLGRPENDGQTSVWYTLLSASSG
                     AHRAEFIPVAYDHERLAAEMRAERLPEEFALTILTGWWTTCLEVLPAKERRRGRF"
     gene            complement(3335..>3581)
                     /locus_tag="VFB80_15650"
     CDS             complement(3335..>3581)
                     /locus_tag="VFB80_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35263.1"
                     /translation="TAHDGNEAALLQILAEWRSADSYAARIQTLRTGAGGLPILDATT
                     VLDDAARDLLVGEAGQECFFAGPADLLPGRQAAEQLN"
BASE COUNT          612 a         1121 c         1196 g          652 t
ORIGIN      
        1 ggtgtcgctg tacttgcttt cgacgggccc ggcagtctgg ctccgcgaca gcggcctgct
       61 gtcgcgcgaa acgtttttcc gcttgtacgc cccggtcgga tggctctatc gccacgttcc
      121 gttcttcagc cagctgctgg aactatactt gcggttctgg tccagttcgt aatcgccatg
      181 cgatacagac tccgcacgct gctcatcgcg ctggccgttg ggccgccgct tttggcgcgg
      241 ccgatcgcat ggctgtggga acacccggct gttttcctgg gtctggtgtt cgctgcgatt
      301 ttcgcgattt tctgggtctc gtgtgggatc atctggttga aggtcgttca gctctttgtg
      361 cacttctccg aagtgccgcg taactaggaa ccgcccatgg tccgcttctc gattcgtgac
      421 gtgctgtggc tgacggcgct ggtcgcgctg gccgtgggct ggtggctgga ccgcagagaa
      481 gtccagcggc tgggcgaagc gcgggcggcg gcgatccggt cgcacgccga agcgttgcag
      541 gcggccctga ggaatgctca gctgtaccaa agcctgacgt ccagcactac gggcacggtc
      601 gtatccagtg gcgctcccgg ctccagttac atcgattacg ggccgatcac accggacgtg
      661 gactggagcc tggccgacaa accgctgccg ccgtaacagc catgaccctc cgctacaaac
      721 tccgcacgct gttgatcctg ctggccgttc tgccgccgct cttgggcgtg gcgtggctga
      781 agtatgccgc gtggaaggcg aagcaagacg agctccagcg ccgccagggc tgggtcatcg
      841 aagtcaggac ttacgtgcaa gagactggct tgccttacac tttggaggac gtgcaaggga
      901 ctgtccctcc ctccgaacca ccgaagccgt gacggaaacg caatcgcatg ttccgctaca
      961 aactgcgcac cctcttgatc ctgctggcca tcctgccgcc gctcttgggc gtggcctggt
     1021 taaagtacgc tgcgtggaag gcggagcagg cccggcgcgc ggaactggcg aagatgcagc
     1081 aactagaaat catctttgac gagatacagc ccgtctacgt gcagcaattg tcgtcgtttt
     1141 ttgatccgcc tgagccattg ccgctggcgg acccgccgtc caactgaact ctaaccggcg
     1201 gcctgctaac gagttgcggc gggagtgggg cggtcacctt gcggcccaag tggtttgaac
     1261 cacggtcagc cttcgggcga ctcacgttcg ggcagcagcc ggcaattgcg gttgcggctg
     1321 ctcgaatgct ggcgaataat tgaatggccg ctggaatgcc ggaaaggata cacgcccgat
     1381 gacgcccttg atgaatcgcc gccgcagttt acagacgctg ggagcactgg cgagcggggc
     1441 cgtcgtggcc ggcctctgcc ggcgggccga ggccgcgaag gcggatccga aggtcgaggc
     1501 ggcgatcgag aagggcctga agtgggtggc caagacgcag tcgtcgctgg gccactggac
     1561 ggcgggaggc tatccgacgg cgatgaccgc actggccggc acggcgctga tcggaagcgg
     1621 ctcgacgaca atccaggggc cgtacgccaa gcacatccgc aagacggtcg actacctgct
     1681 cggcaagctg cggcccaacg ggctgatcgg cgatccggcc tccgacaacc gctacaccta
     1741 cggccacggc ttctcgatgc tattcctctc gcaggtcctc ggcgaagagg aagacgagga
     1801 gcgccgcgac gagctggtca ccgcgctcga gaaggcctgc gacttcagcg gcaaggccca
     1861 gacgccctct ggcggctggg gctacatcag cgcgaaggac ggcaataact tcgacgaagg
     1921 ctcgaccacc attacgcagg tgcaggggct tcgcggctgc cgcaacggcg gcatcgtcgt
     1981 cccgggcgac gtgatcaaca aggccaaaga atacatttac ggctgcaaga accccgacgg
     2041 cgggatttcg tacagctcgc ggaaccgcgg cagctcgcgg ccggcaatca ctgcggcggc
     2101 cctcgccgcc ctctataacg cgggcgacta cgacagcaag cacgtgccgg agatgctcga
     2161 gtattgcaag aagacgctgc agaacctgag cgaagggccc aattcgttcg gccactggca
     2221 ctacacgtat ctctattttt cgcaggtcat gtaccggcag gggaacaagg agtgggagcc
     2281 gttccgcgac aagctctatt cccacatcgt caaggagcag ctgaacgacg gctcgtggca
     2341 gggcaacttt ggcccgatct atcccacgag ttgcaacctg atcatgctcc agctcgacaa
     2401 ggcgtatttg ccgatctatc agaggtgagc cgcgccgtag ggaacgccct ctgtggcgtt
     2461 ccgatgtgcg agtggtgcaa ctttatcaga acctgccgcg ccgccgttcc tttgcaggca
     2521 gcacctccag gcaggttgtc caccagccgg tgaggatggt tagggcgaac tcttccggca
     2581 ggcgctcggc ccgcatttcg gcggcgaggc gctcgtggtc gtaggcgacg gggatgaatt
     2641 cggcgcggtg ggcgccgctt gaggccgaca gcagcgtgta ccagacgctg gtttggccgt
     2701 cgttttccgg gcggccaagc acgccgacgt tgaggaagag ctttccgccg ggcagttctc
     2761 gctgccactt caggccggtg tgcgtcgtac agatgacatc ggccgcgtat tgttcgcaga
     2821 gggccgacag gaaatgcgtc gagctggtcg attcccagag gaattcgttc atccgccgcg
     2881 ggccgccgtg gcagagcagc actcgccggc cgcctagctc caggcgaatt tcggccggca
     2941 gctcgcgcag ccacgcgcgg ttagcaggcg acgtattcgc cagcgtgtag tcgtagctga
     3001 tctgagcgaa gtggttgtcg cgggggtccg tgtagccgca ttggcaatcg gccagttcgc
     3061 ggccgatcga gtcgtcgtag ttgccgcgga cgcaggccac gccgtgctcg cgcagcagtt
     3121 cgatcgcgcg gtcgggatgt gggccaaaag cgcccaggtc gccgaggcag tagacgtccc
     3181 gcacgccgcg ccggcggatg tcggccagcg cggcctccag ggccaggtag ttgctgtaga
     3241 tgccgccgag cagggctgct ggccgcgggc cctgcccggc gtcgtcgtca tgaacgcgcc
     3301 tgctcacggt cgcattctat cctgcgccgc tgcgtcaatt cagttgctcg gcggcctgcc
     3361 tgccgggcag caggtcggct gggccggcga agaaacactc ctggccggcc tcgccgacga
     3421 gcaggtcgcg ggcggcatcg tccagcacgg tcgtcgcgtc caggatcggc agtccgcccg
     3481 cgccggtgcg gagtgtctgg atgcgcgcgg cgtaggaatc ggccgaacgc cactccgcca
     3541 gaatttgcag cagcgcggct tcattgccgt cgtgggcggt c
//
LOCUS       DASRJB010000592         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3834964, whole genome shotgun sequence.
ACCESSION   DASRJB010000592 DASRJB010000000
VERSION     DASRJB010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834964"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1195
                     /locus_tag="VFB80_15655"
     CDS             152..1195
                     /locus_tag="VFB80_15655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZN35264.1"
                     /translation="MLQGMLGQIHQGDCIAGMNGLPEGCIDLAFADPPFNIGYEYDVY
                     DDKKEHEHYLDWSRQWIAAVYRVLKPDGTFWLAIGDEYAAELKLLSQDIGFHCRSWVV
                     WYYTFGVNCKSKFTRSHAHLFHFVKDPRKFTFRSEELENRIPSARQLVYADARANPVG
                     RLPDDTWILRPQDLAGSFQADEDTWYFPRVAGTFKERAGFHGCQMPEQLLGRIIRLCS
                     HAGETVLDPFSGSGTTLAVAKKLARNYVGFDLSPEYVAQGEERLRFIQPGDPLVGAPE
                     PTMSAPATRAGKRLADKLAKPNGNGKSHRANGKGVTVGGKKRAAGANRMEELPLFSRK
                     KSRARKPGRKAAK"
     gene            1259..1864
                     /locus_tag="VFB80_15660"
     CDS             1259..1864
                     /locus_tag="VFB80_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZN35265.1"
                     /translation="MPTIDQPTLDIVISICLGLGLSAACGFRVFVPLLGLSVAAMSGY
                     VQPAAGWEWVGSWPAVACLATATVLEIAAYYVPWIDNALDSIATPAAVIAGTLATASV
                     VTDLPPLWRWSLAAIAGGGIAGLIQAATVTLRGTSTVTTGGLANFLVSTGELILSLVT
                     TLVALLLPVLGAGLVLALLLWITLKLTNRRAKVPPSARPAL"
     gene            complement(1888..>2365)
                     /locus_tag="VFB80_15665"
     CDS             complement(1888..>2365)
                     /locus_tag="VFB80_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN35266.1"
                     /translation="IKELTEGAEDKAALRGKLHQAALALAAEAEFGSQHGAVPVAAGE
                     IEAGRKLIAGELGCTDCHRFHDAGELGNAPDLTGYGSRAWLAKMIANPQGERFYAGDR
                     NDRMPAFAADPQQPKHNLLGPQELDLVVAWLRGEWHEPERLAADDSAKATAEAARK"
BASE COUNT          397 a          766 c          765 g          437 t
ORIGIN      
        1 aatccaaagc ggtcgaacca tcacgatctt cctcctggct tctattgtgg ctggccggag
       61 gcgatccatc cagcggcggt tggaggctga ttgcaacttg cgctttagga cgtgcgacga
      121 tatattgagg ggcatcttgg aggaggaagt catgttgcag ggaatgctgg gccaaattca
      181 tcagggtgat tgcatcgcgg ggatgaacgg actgccggag ggctgcattg acctggcgtt
      241 cgccgatccg ccattcaaca tcggctacga gtacgacgtc tacgacgaca aaaaggagca
      301 cgagcactac ctcgactggt cgcggcagtg gatcgccgcc gtgtatcgcg tcctcaaacc
      361 cgatgggact ttctggctcg ccatcggcga cgaatatgcc gccgagctga agctgctgtc
      421 acaggacatc ggcttccatt gccggagctg ggtggtctgg tactacacat ttggcgtgaa
      481 ctgcaagtcg aaatttaccc gctcgcacgc ccacctgttt cacttcgtca aggacccgcg
      541 gaagttcaca ttccgcagcg aggagttgga aaaccgcatc ccgtcggcgc ggcagctcgt
      601 gtatgccgac gcgcgggcca atccggtcgg ccggctgccg gacgacacgt ggattctgcg
      661 gccgcaggac ctggccggat ccttccaggc ggacgaggac acgtggtact tcccgcgagt
      721 cgccggcacg ttcaaggagc gggccggctt ccacggctgc cagatgccgg agcagcttct
      781 gggtcggatc atccgcctct gctcgcacgc aggggaaacg gtcctcgatc cgttttccgg
      841 cagcggcacg acgctggccg tggccaagaa gctcgcgcgg aattacgtcg gcttcgatct
      901 gtcgcccgaa tacgtcgccc agggggaaga acggttgaga tttattcagc cgggtgatcc
      961 gctcgtcggc gcgcccgagc cgacgatgag cgccccggcc acgcgggccg gcaagcggct
     1021 ggccgacaag ctggccaagc ccaacggcaa cggcaagtcg catcgtgcca acggcaaggg
     1081 agttacggtc ggcggcaaga agcgggcggc aggggcgaat agaatggaag aactgccgct
     1141 gttttcccgg aagaagtccc gggcgcgaaa gcccggccga aaagcagcga agtgaatttt
     1201 gtaggacggc cttccaaggc cgttcatgcg acggactagg aagtccgtcc tacgactgat
     1261 gcccacaatc gaccagccca ccctcgacat cgtcatctcg atctgcctcg gcctggggct
     1321 gagtgctgcg tgcggctttc gtgtgtttgt gccgctgctc ggactgagcg tggcggcgat
     1381 gagcggctac gtgcagccgg cggctggctg ggaatgggtc ggctcgtggc ccgcggtcgc
     1441 ctgcctggcg accgcgaccg tgctggagat tgccgcctac tacgtccctt ggatcgacaa
     1501 cgctctggac tcgatcgcca cgccggcggc agtgattgcc ggcacgctgg ccacggcctc
     1561 ggtcgtgacc gatctgccgc cgctatggcg gtggtcgctg gcggcgattg ccggcggcgg
     1621 cattgccggc ctgatccagg cggcgacggt cacgctccgc ggcacttcca ccgtcaccac
     1681 cgggggtctg gcgaattttc tggtatcgac cggcgagctg atcctgtcgc tggtcacgac
     1741 tctcgtagcg ctgctgctgc cggtgctcgg ggcggggctg gtactcgcct tactgctctg
     1801 gatcacgctt aagctgacaa accggcgcgc aaaagtacct ccgtcggcgc ggcccgctct
     1861 gtgacgagag tgccgcttgc ccccgcgtca cttgcgagcc gcttccgccg tcgccttggc
     1921 ggaatcgtcg gcagccagtc tctccggctc gtgccactcg ccgcgcagcc aagccacgac
     1981 gaggtccagc tcctgcgggc caagcagatt gtgcttgggc tgctgcggat ccgcggcaaa
     2041 tgctggcatc cggtcgttgc ggtcgccggc ataaaaccgc tcgccttgag gattggcgat
     2101 catcttcgcc agccaggccc gagagccata gccggtaagg tccggcgcgt tccccagctc
     2161 gcccgcatcg tgaaagcgat gacagtccgt gcaacccaac tcaccagcga tcagctttcg
     2221 ccctgcttcg atctcccccg ccgcgaccgg caccgcgccg tgctgtgagc cgaattccgc
     2281 ttccgccgcc agggccagtg ccgcctggtg cagcttgccg cgcagcgccg ccttatcttc
     2341 cgcgccttcc gtcagctctt tgatg
//
LOCUS       DASRJB010000593         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2137574, whole genome shotgun sequence.
ACCESSION   DASRJB010000593 DASRJB010000000
VERSION     DASRJB010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137574"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFB80_15670"
     CDS             <1..257
                     /locus_tag="VFB80_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35267.1"
                     /translation="FLVAGLTAAALLEAEAPTLKVALLLAICIWCFCRAYYFAFYVIE
                     HYVDPQFKFAGLTSFVIYLVRGSRMGARDVTSVDGGENAR"
     gene            complement(223..1797)
                     /locus_tag="VFB80_15675"
     CDS             complement(223..1797)
                     /locus_tag="VFB80_15675"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZN35268.1"
                     /translation="MYDLLFTGGTVIDGTGSPRVSADVAVSGDRIAAIGPLSGADARR
                     TIDCRGLVVAPGFIDVHNHSDGWMVKEPHQTWKTSQGFTTEVLMADGISYAPVDEQNW
                     RQWIYYLRSLNGLSLADYRGWKSLGDYLSLIDGRNVQNCAAHVPYANVRVLACGFGRN
                     IPPDDVQLREIKARIRQGMEDGAVGLSTGLDYVNQCHAMTDEIAAACTAVVPYGGLYV
                     THVRYKIGLLPALREAVEIGRRSGVKVHISHLKGSSEQEVQEVLAFIDNEARRQVDFS
                     FEVYPYQRGSTMLNYLLPYEVWADGPLGVLAKLDRPEIRSRFRAALDAFGVPLSQMTI
                     AWTAMPGGESLLGMPLDEFVRQRGKEPHEALIDLLIDSNLGTLLVAGPPTDHLVEPLV
                     QHDLAILGSDGIYQPGGHVHPRVYGSAARWLGPFVRDRKLFSLETAVHKLSGQSAARY
                     GLADRGVIRQDAFADLVVFDPATIADRATYENPRQTSTGVSTVAVNGQVIVENGQPSA
                     LPAGPLSGRFLRHQLK"
     gene            1956..2540
                     /locus_tag="VFB80_15680"
     CDS             1956..2540
                     /locus_tag="VFB80_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35269.1"
                     /translation="MWQIDRRLLYVAIAVGLVAIAVLSITYANDWVLSGLATLSIGCW
                     LTGVLWTVYAPPKDRAFPLGALAASFIYVLLACGPWFQSNVGPWLLTTRALVTIDTHL
                     LGREQPQVAYQEVPLSTIYSNNLSNTLSFPPNNLWTTARMPASASAVTPSVAIGHWLF
                     AWCAAAIGGFAARWMVRRQQRTSSGGSPFAEAAR"
     gene            2537..3142
                     /locus_tag="VFB80_15685"
     CDS             2537..3142
                     /locus_tag="VFB80_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35270.1"
                     /translation="MKFSLLTLLVITAFAGLACAALVKPSVDWLTAVVSLTALAFIYQ
                     VLRALVQRGASQAAAAGWLLFASTYLLLVFCPWLGERVGPDLITSRAILAAQQRWLPE
                     SNSVELQLAFVPDGGLVASVGWPLQTTVNVNSNASYALVRSLLTAPNRLPHALTPAEL
                     FCRTAHWLCAWLAGGLGAAVALVCYRRRHGAPRGAASLPAR"
     gene            3196..>4993
                     /locus_tag="VFB80_15690"
     CDS             3196..>4993
                     /locus_tag="VFB80_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZN35271.1"
                     /translation="MPSALTLSFPEPDIALVTFDLPGKGANILSRSVLQELAAILDQL
                     QPRRDLAGLVIRSGKPGTFIAGADIREFLASLGAPKSEVVAMGRRGQQLFQRLSQLNM
                     VTVAAIDGVCVGGGAELSIWCDRRVMSDNPKTEYGFPEVKLGLYPGWGGTVRAPRIVG
                     LANALEMITTTENVDSRAALTMGLASDVVPAEKLLDAAIRVIRAEKQSGQYLKDRERW
                     RQPMEVNPTELGFLGATASAMIQNETKGHYPAPLAALELMLETCQQPAEEALEKEAAG
                     MAELFGSPVNAALINVFFLTDRNKRDTGLDKTGVNAANIKSVGVIGSGIMGSGIAGAA
                     LKSELPVALTDANTEALARGAKQLLEEAAYNRKLKGTDPSKALKLAPLLSVTHSDEEI
                     AKRDLVVEAIIENPDAKKQLYARLEPLLRPDALLASNTSTIPITRLAEGLKRPERFCG
                     IHFFNPVRRMKLVEVIRGAKTSDETIATAVAYAKRIGKSPIVVNDGPGFLVNRLLFPY
                     MNEAIELLSDGASIKEIDRAATAFGMPMGPITLYDLVGLDTAMYAGMVMYQAFPDRVV
                     ASPLVPSLVKQGRLGQKSGKGFFSYENKKGRAE"
BASE COUNT          831 a         1612 c         1679 g          871 t
ORIGIN      
        1 tgttcctggt cgcgggcctg accgcggcgg cgctgctcga agccgaagcg ccgacgctca
       61 aggtcgccct gctgttagcc atctgcatct ggtgcttctg ccgcgcgtac tacttcgcgt
      121 tttacgtgat cgagcactac gtcgatccgc agttcaagtt cgccggcctg acttcgttcg
      181 tgatctacct ggtgcgggga tcgcgcatgg gggcgcgcga tgtcacttca gttgatggcg
      241 gagaaaacgc ccgctgagtg gcccagccgg cagcgcggag ggctggccgt tctcgacgat
      301 cacctggccg ttgacggcga ccgtggaaac gcccgtgctc gtctggcgcg ggttttcgta
      361 cgtggcgcgg tcggcgatcg ttgccgggtc gaagacgacc aggtcagcga aggcgtcctg
      421 gcggatgacg ccgcgatcgg ccaggccata ccgcgcggcc gactggccgc tgagcttgtg
      481 cacggccgtt tccagcgaga acagcttccg gtcgcgaacg aaaggtccta gccagcgggc
      541 cgcggagcca tagacccgcg gatgcacgtg gccgcctggc tgatagatgc cgtcgctgcc
      601 caggatcgcc aggtcgtgct gcaccagcgg ttctaccagg tggtccgtgg gcgggccggc
      661 gacgagcagc gtgccgagat tcgagtcgat gagcagatcg atcagcgcct cgtgcggctc
      721 cttgccgcgc tggcggacga attcgtcgag cggcatgccg agcagcgatt ccccgccggg
      781 catcgccgtc caggcgatcg tcatttgaga aagcggcacg ccgaacgcat cgagcgccgc
      841 gcgaaatcgg ctacggattt ccggccggtc gagcttcgcc aagacgccca gcggcccgtc
      901 ggcccagacc tcgtagggca gcaggtagtt gagcatcgtc gagccgcgct ggtacgggta
      961 cacctcgaac gagaaatcga cctgccgccg ggcctcgttg tcgatgaagg ccagcacctc
     1021 ctgcacttcc tgttcgctcg agcccttgag gtgcgagatg tgcaccttca cgccagaccg
     1081 gcggccgatc tcgacggctt cgcggagggc gggcagaagg ccgatcttgt agcgcacgtg
     1141 cgtgacgtag agcccgccgt aagggaccac cgcagtgcac gcggccgcga tttcgtcggt
     1201 catcgcgtgg cactggttga cgtaatccag gcccgtcgag aggccgaccg cgccatcttc
     1261 catcccctgg cggatgcgag ccttgatctc gcgcagctgc acgtcgtcgg gcgggatgtt
     1321 gcggccaaac ccgcaggcca gcacgcggac gttggcgtag ggcacatgcg ccgcgcagtt
     1381 ctgaacgttc cgcccatcga tcagcgacag gtaatcgccg agcgacttcc agccgcgata
     1441 atcggccaag gacaggccgt tgagcgaccg caggtaatag atccactgcc gccagttctg
     1501 ctcatcgacc ggggcgtagc tgatcccgtc ggccatcagc acttcggtcg tgaagccctg
     1561 gctggtcttc caggtctggt gcggctcctt gaccatccag ccgtcgctgt ggttgtggac
     1621 atcgatgaag ccaggcgcga caacgaggcc gcggcagtcg atcgttcgcc gggcgtccgc
     1681 gccgctcaga gggccgatcg cggcgatccg atcgcccgaa accgccacat cggcggaaac
     1741 ccgcggcgag cccgtcccgt caataactgt gccgccagtg aatagcaggt cgtacaagtg
     1801 aacgtgtcct tagcccggac cgaacctggt ccccaagccg gggaccacgt cgatccgcaa
     1861 ttcaccggga gcaggtttgc tcccggctag tgatttgtcg gacgtaatgc gagaattgta
     1921 atcggacttg gggcgagcgc cagcggagac gaactatgtg gcagatcgat cgccggctgc
     1981 tgtatgtggc gattgccgtc ggcctggtgg cgatcgcggt tctctcgatt acctacgcca
     2041 acgactgggt gctcagcggg ctggcaacgc tctcgatcgg ctgctggctc acgggcgtgc
     2101 tctggaccgt ttacgcgccg ccaaaggatc gcgcctttcc cctcggcgct ttggcggcca
     2161 gcttcatcta tgtgctgctg gcctgtgggc cctggtttca atcgaacgtc ggcccctggc
     2221 tgctcaccac gcgggccctc gtcacgatcg acacgcatct gctgggtcgc gagcagccgc
     2281 aggttgctta tcaggaagtg ccgctctcga cgatctattc gaacaatttg agcaacaccc
     2341 tgtcctttcc gccgaacaac ctctggacca cggcgcgaat gcctgccagc gctagcgccg
     2401 taacgccgag tgtggcgatt gggcactggc tgttcgcgtg gtgcgcggcg gcgatcggcg
     2461 gcttcgccgc ccgctggatg gtccgccggc agcagcggac cagcagcggg ggcagcccgt
     2521 tcgcggaggc ggcacgatga agttctcgct gctcacgctg ctggtcatta cggcgtttgc
     2581 aggcctggct tgtgcggcct tggtcaagcc cagcgtcgac tggctgacgg ccgtcgtcag
     2641 cctgacggcc ctggcattca tctatcaggt gctgcgggcc ctcgtgcagc gcggagcctc
     2701 gcaggccgcg gccgccggct ggctgctgtt tgcctcgact tacttgttgc tggtgttttg
     2761 cccgtggctc ggcgagcgcg tcgggccgga cctgattacc tcgcgggcga ttctcgcggc
     2821 tcagcagcgg tggctgccgg aatccaatag cgtcgagttg cagctggcct tcgttccgga
     2881 cgggggcctg gtggcgtcgg tcggctggcc cttgcaaacg acggtgaacg tgaattccaa
     2941 cgccagctac gcgctggttc gcagcctgct gacagcccca aatcggctgc cgcacgcgct
     3001 cacccctgcg gagctgtttt gccggaccgc acattggctg tgcgcctggc tggccggagg
     3061 gctcggcgca gccgtcgccc tggtctgcta tcgccgccgg cacggagcgc ctcgcggcgc
     3121 agcctcgctt ccagcacggt agcgcacgcg gtagcatgca cgcagcctgg ctccgcctct
     3181 catggaatgc aatgaatgcc tagcgccctg actctttcgt tccccgagcc cgacattgcc
     3241 ctggtcacgt tcgacctgcc gggtaaaggg gccaacattc tgtcgcggtc ggtacttcag
     3301 gagctggcgg cgatcctgga ccagctgcag ccgcggcgcg acctggcagg cctggtgatc
     3361 cgctcgggca agccgggcac gttcatcgcc ggggcggaca ttcgcgagtt tttggcgtcg
     3421 ctcggagcgc ccaagagcga agtcgtggcc atgggccgcc gcgggcagca gctctttcag
     3481 cggctgtcgc agctgaacat ggtcaccgtg gcggcgatcg acggcgtctg cgtcggcggc
     3541 ggggcggagc tgtcgatctg gtgcgaccgc cgcgtgatgt cggacaatcc caagaccgag
     3601 tacggctttc ccgaggtcaa gctggggctc tatcccggct ggggcggcac ggtccgcgcg
     3661 ccgcgcatcg tcggcctggc gaacgccttg gagatgatca cgaccaccga gaatgtcgat
     3721 agccgtgccg cgctgacgat gggactggcc agcgacgtcg tgccggccga gaagctgctg
     3781 gacgcggcca ttcgcgtgat tcgagcggag aagcaatcgg gccagtacct gaaggaccgc
     3841 gagcgctggc ggcagccgat ggaagtgaac ccgaccgagt tgggtttcct tggcgcgacc
     3901 gcctcggcca tgatccagaa tgagacgaag ggccactatc cggcgccgct ggccgccctg
     3961 gagttgatgc tggagacctg ccagcagccg gcggaggagg ccctcgaaaa ggaagccgcc
     4021 ggaatggccg agctgtttgg ctcgccggtg aatgcggccc tgatcaacgt ctttttcctc
     4081 acggaccgga acaagcgcga cacgggcctc gacaagacag gcgtcaatgc cgcgaacatc
     4141 aagagcgtcg gggtgatcgg cagcggcatc atgggcagcg ggattgccgg ggcggcgctc
     4201 aagagcgagc tgcccgtcgc gctgaccgat gcgaataccg aagcgctggc ccgcggcgcg
     4261 aagcagctgc tggaggaagc agcctacaac cgcaaattga aaggcacgga ccccagcaag
     4321 gcgctcaagc tcgcgccgct gctctcggtc acccatagtg acgaggagat tgcaaaacgc
     4381 gacctcgtgg tcgaggcgat catcgagaac cccgacgcca agaagcagct ctatgcccgg
     4441 ctggagccgc tcctcaggcc cgacgcactg ctggcgagca acacctccac gattccaatc
     4501 acgcggctgg ccgaggggct caagcggccc gagcggttct gcgggattca cttcttcaac
     4561 cccgtgcggc gaatgaagct ggtcgaggtg attcgagggg cgaagaccag cgatgagacg
     4621 atcgccaccg ccgtggccta tgccaagcgg atcggcaaat cgccgatcgt cgtcaacgac
     4681 gggccgggct tcctggtgaa ccggctgctc tttccttaca tgaacgaagc gatcgagctg
     4741 ctaagcgacg gagcctcgat caaggagatc gaccgcgcgg cgacggcgtt cggcatgccg
     4801 atgggtccga tcacgctcta cgacctcgtg ggcctcgaca cggcgatgta cgccggcatg
     4861 gtgatgtacc aggcgtttcc cgaccgtgtc gtcgcctcgc cgctggtgcc gtcgctcgtc
     4921 aagcaaggcc gcctcggcca gaaatcgggc aaggggttct tctcgtacga aaacaaaaag
     4981 ggacgagccg aag
//
LOCUS       DASRJB010000594         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3555042, whole genome shotgun sequence.
ACCESSION   DASRJB010000594 DASRJB010000000
VERSION     DASRJB010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555042"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..935
                     /locus_tag="VFB80_15695"
     CDS             153..935
                     /locus_tag="VFB80_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35272.1"
                     /translation="MLQIRLGVELASLQSPLRQALLTARELGVEAVEIDARTQLRPEE
                     LSHTGVRQVRKMLDDLNLRVSAVAFRTRRGYHVLEDLDVRLDATRRAMKLAYDLGTNV
                     LVNHVGRVPAEPDSPAFNTLVQALADLGRYGQRVGAMLSAETGTESGADLAGLIARLP
                     PGSIGVDFNPAGLILNGFSPREAIAALAPHVLHLHAIDATRDLALGRGMEVPLGQGNA
                     ELPEILAALEEQQYRGYITVARHQSTSPRADIQQAIEFLRNL"
     gene            complement(1040..2092)
                     /locus_tag="VFB80_15700"
     CDS             complement(1040..2092)
                     /locus_tag="VFB80_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35273.1"
                     /translation="MRVSFTRSLFLGTVCVSLMACAGGCRSGGTGWTTPSWMNWSNWT
                     GGGSTTAPSTSLSQNTRPGMGVAKPSTQVSPQATASVAAGTGGTGAAGGYPGGYTGAT
                     NPRYTTTTSPPPSYGAGTSGYGAAPASAYQQASTSGQVQPTVGYQTGPYSMTSAAAPQ
                     NPYAQSGYGQNNAAAGSTGGSTWSAPAAAQPAAAPANNYGASAYTADQRGMYGAQEAS
                     PYASDPSRQGYSTGQPAYGQQPAAAPSAATGPWQNYTQPAGGYQSSAQPAGAAGTAAY
                     DPSAAAAYAAAAAAAASQPAATTAAASGYPAVPAALATDASSFKPGSTGGYGVQNAAY
                     QQQPATAQTYGNTYQR"
     gene            2480..2782
                     /locus_tag="VFB80_15705"
     CDS             2480..2782
                     /locus_tag="VFB80_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35274.1"
                     /translation="MDFNRNQFFMAGLVVVLLGIQLRLVDTFVLNERTTQFLAQRMQQ
                     LKGQQVASASDVPTLFAAQAPVHKHRLKLPPWIGWACVSVGSVLILHSLALKKPGG"
     gene            complement(2786..>3547)
                     /locus_tag="VFB80_15710"
     CDS             complement(2786..>3547)
                     /locus_tag="VFB80_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35275.1"
                     /translation="PASGVSEATQAARYAAIAELNRRHLAERGRDDALESRLRSYELA
                     ARMQLSIPEATDLAQETAATHALYGTDQKDSADFGRGCLLARRLIERGVRFVQLWSGA
                     AFGTEVHWDAHGSVPTNHRRESAKIDRPVAGLLCDLRQRGLLDDTLVIFNTEFGRTPY
                     AESAGDKAGPGRDHNADAFSVWLAGAGLKHGLAYGTSDDIGWKAAENPVDVHDFHATI
                     LHLLGIDHTRLTFYHNGIRRRLTNVHGRVLKDILA"
BASE COUNT          549 a         1200 c         1211 g          587 t
ORIGIN      
        1 aggagcgata aaatgagggg cagatacata agaaccaaag aaccaaagaa ccaaagaacc
       61 aaagaaccaa agaaccgaag aaccgaagaa ccgaaggata cgcactctgc taatttggtt
      121 ctttggttct tgagatttcc cgggaggggc tagtgcttca gattcgtctc ggcgtcgagc
      181 tggccagcct gcaatcgccg ctcaggcaag ccttgcttac ggctcgcgag ctgggcgtgg
      241 aggcggtgga aatcgacgcc cgcacgcagc tgcggcccga ggagctctcg cacactggcg
      301 tgcggcaggt gcggaagatg ctggacgatc tgaatttgcg ggttagtgcg gtcgccttcc
      361 gcacccgccg cggctaccac gtcctcgaag atctcgacgt ccggctcgac gctacgcggc
      421 gggcgatgaa gctggcttac gacctgggga cgaatgtgct ggtcaaccac gtcggccgcg
      481 tccccgccga gcccgattcg ccggcgttca atacgctcgt ccaagcgctg gccgacctcg
      541 gccgctacgg gcagcgcgtc ggggccatgc tgtccgccga gaccggcacg gagagcggcg
      601 cggatctggc cggcctgatc gcccgcctgc cgcccggatc gatcggcgtc gatttcaatc
      661 cagccgggct cattctgaac ggcttctctc cgcgcgaggc aatcgcggcg cttgcgccgc
      721 acgtgctgca cctgcacgcc atcgacgcga cccgcgactt ggcgctaggc cgcggcatgg
      781 aagtgccgct gggccagggc aacgccgagc tgccggagat tctcgcggcg ctggaagagc
      841 agcagtaccg cggctacatc accgtcgccc gccatcaaag cacatcgccg cgggccgaca
      901 tccagcaggc gatcgaattt ctgcggaacc tgtaatccgc cgtaggcgac gctgccagcg
      961 tcgcccaaag ccgcgaccag tggtcgcggt gcactggtag accgcgacca ctggtcgcgg
     1021 ctttagaaca aatagcgggc taccgctgat aggtattgcc gtaggtctgg gcggtggccg
     1081 gctgctgctg gtaggcggcg ttctgcacgc catagccgcc cgtgctgccg ggcttgaagc
     1141 tgctggcgtc ggtcgccagt gcggccggaa ccgccggata gccgctcgcc gcggcggtcg
     1201 tggccgccgg ctggctcgcg gctgctgcag cagcagccgc ataggcggcc gccgccgacg
     1261 gatcataggc cgccgtgccc gctgcacccg ccggctgggc gctgctctga tagccgccag
     1321 ctggctgcgt gtagttctgc cacggtcccg tggcggccga cggcgccgca gcaggctgct
     1381 ggccataggc cggctgcccg gtcgaatagc cttgccgcga ggggtcgctg gcataggggc
     1441 tggcttcctg agcgccgtac atgccgcgct gatcggccgt gtaagcactc gcgccgtagt
     1501 tgttcgctgg ggcagcggca ggctgagctg cagccggcgc cgaccaggtc gagccgccag
     1561 tcgagccggc cgccgcgttg ttctggccgt agccgctctg ggcatatggg ttctgcggag
     1621 cggccgcgct ggtcatgctg tacggcccgg tctgatagcc gaccgtcggc tgcacctgcc
     1681 cggaggtgct ggcctgctga taggccgatg ccggggcagc gccgtagccg gaagtcccgg
     1741 cgccatacga cggcggcggg ctcgtggtgg tcgtgtaccg cggattcgtc gcgccggtgt
     1801 agccgcccgg atagccgccg gccgcgcccg tgccgcccgt tcctgcggcg acgctggccg
     1861 tggcctgcgg cgagacctgc gtcgagggct tggcaactcc catgcccggc ctggtgttct
     1921 gggacagcga ggtggagggc gccgtggtgc tgccgccgcc ggtccaattg ctccaattca
     1981 tccagctcgg cgtcgtccag cccgtgccgc ccgaccggca gccgccggcg cacgccatca
     2041 ggctcacgca gaccgtaccg aggaacaacg aacgagtaaa cgagactcgc atggctagat
     2101 ccttgacaaa agccgccggc cgtgggccga ggtagattcc attctgctga ttgctgggcc
     2161 aagctcgccg cgggggcggc gctctcattt tccttgtcgg ccggaaatac cggcgcattc
     2221 cacacaatcc gcacagattg ccaaaaaatc tcgcgcgcac ttggcgcgcg tggcactccg
     2281 cagcggggcg ggatggtagg cgcggctgcc ggatgaggtc aacggcaaat tccacggtcg
     2341 cagcgcccca agacgctagc tatcgcgcaa ccgcgccatt ccgtgctatt ggacggttgt
     2401 tgcagcacgc ccctgacgca attcgatact ccgataacag cgccggcttt ttttccgctg
     2461 ccgcttgccc gaggcgtcga tggatttcaa ccgcaatcag ttcttcatgg ccgggctggt
     2521 cgtggtcctg ctcggcattc agttgcggct ggtcgatacg ttcgtactca acgagcggac
     2581 gacgcagttc ctggcccagc ggatgcagca gttgaaaggg caacaggtcg ccagcgccag
     2641 cgacgtgccc acgctcttcg ccgctcaggc ccccgtccac aagcaccgcc tgaagctccc
     2701 gccctggatc ggctgggcct gcgtctccgt cggcagcgtg ctcatcctgc acagcctcgc
     2761 gctgaaaaag cccggcggct gagaatcacg ccaggatgtc cttcagcacg cgaccgtgca
     2821 cgttcgtcag gcggcggcga atgccgttgt gatagaacgt cagccgcgtg tggtcgatgc
     2881 ccaagaggtg caggatcgtg gcgtggaagt cgtggacgtc caccgggttc tcggcggcct
     2941 tccagccgat gtcgtcggac gtgccgtagg ccaggccgtg cttgagcccc gcgccggcca
     3001 gccagacgct gaaggcgtcg gcgttgtggt cgcggccggg gcccgccttg tcgccggcgc
     3061 tctcggcata gggcgtgcgg ccgaactcgg tattgaagat caccagcgtg tcatccagga
     3121 ggccgcgctg ccgcaggtcg cagagcaggc ccgcgacggg ccggtcgatc ttggccgatt
     3181 cgcggcggtg attggtaggc acgctgccgt gggcgtccca gtggacctcg gtgccgaacg
     3241 ccgcgccgct ccagagctgc acgaaccgca cgccccgctc gatcagccgc cgggccagca
     3301 ggcagccgcg gccgaaatcg gccgagtcct tctggtccgt gccgtacagc gcgtgcgttg
     3361 ccgccgtttc ctgggccagg tccgtcgcct cgggaatcga cagctgcatc cgcgccgcca
     3421 gctcgtagct ccgcagccgg ctctccaggg cgtcgtcgcg gccgcgctcg gccaggtggc
     3481 ggcggttcag ctcggcgatc gcggcatagc gggccgcctg cgtcgcttcg ctcacgcccg
     3541 acgccgg
//
LOCUS       DASRJB010000595         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2427947, whole genome shotgun sequence.
ACCESSION   DASRJB010000595 DASRJB010000000
VERSION     DASRJB010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427947"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFB80_15715"
     CDS             <1..331
                     /locus_tag="VFB80_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylgalactosamine-6-sulfatase"
                     /protein_id="HZN35276.1"
                     /translation="MVPTLRGNSSQQKQHDFLYWTFYERGGGRAIRAGDWKLVQQPLS
                     SSERLYNLAQDIGEERDLAAEQRQLVAQLTTKMDSAYVPHENWKFPAPGAAKAKTGGK
                     QKAKSPQ"
     gene            338..1264
                     /locus_tag="VFB80_15720"
     CDS             338..1264
                     /locus_tag="VFB80_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35277.1"
                     /translation="MKTYSHSGWAPPHGVFATLFAGMAAAVPFGFIYAYSFRYIPYPY
                     LNIVITLAFGAGLGFIVAKTAEWGKVRNNIIVGAIGLFVGLFGLYVYWGAYWWAVAGF
                     DRVAQIGLYAFMPWGILDLALRLFDKGSWGISENAGNLTGWLLVGIWVIETCIVLGLS
                     VVVSLLNSDRPFCETCQVWTESQRGVARLSGNGSEPAWEKVLAGELPALAEFPPAPPG
                     AMQYVRLDLARCPRCEYGRFLTICAVDVVVDKEGKASEKARALVTNGIVTPSQCAVVE
                     ACGKLYDQHLEQSLGPLSAEAGGPPAPAEGES"
     gene            1264..2700
                     /locus_tag="VFB80_15725"
     CDS             1264..2700
                     /locus_tag="VFB80_15725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN35278.1"
                     /translation="MAPFRLPSCAAIALAMLATLAEAAERPNFVVLLCDDLGYGDLGC
                     YGHPQIKSPRLDKLATEGIRFTECYAAAPVCSPSRAGLLTGRNPGRAGIYDWISNNHP
                     MHLGANEVTIATLLKQAGYATCQIGKWHLNGKFNQADQPQPGDHGFQHWMSTQNNAGP
                     SHANPRNFVRNGTPLGDQQGYSCDLVTAEAIRWLSDLRDKGKPFFLYVCYHETHEPVA
                     SPADLVAQYPDAKKPGEAEYYANVANVDRAIGRLLDKLDELKLSERTLVFFTSDNGPE
                     TLLRYASGKHCHGSPGALRGMKLHLYEGGIRVPGILRYPTRVKGGQTIDEPVCSLDFL
                     PTFCELGGVKLPEGRPLDGASFTPLFDGQPIVRTTPLYWQYFRSIGEPKAALRVGDYV
                     ILGKWDGPQLPPGAGLAHGDCEIIKAAKLSGFELYNLKTDPGQKTDLAAAEPGKLKEL
                     SDLLVAKYQEVQKEGPVWKVPKPVGKEK"
     gene            3109..4017
                     /locus_tag="VFB80_15730"
     CDS             3109..4017
                     /locus_tag="VFB80_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZN35279.1"
                     /translation="MRRTLTVLTCTFLAFASSALGQEKAGRRYPPDLPGAKVVTYKTV
                     GDTKLNLWIYEPEGHKPEDKSPAIVFFFGGGWTGGSPQQFEQQCKHLAGRGMVAMTAD
                     YRVASRNQVKAVSCVADAKSAVRYIRQNAARLGIDPSRIAAGGGSAGGHLAACTAALK
                     GFDEAGEDASVSSVPNALALFNPAVVLAAVDGIRELDAAKVSSFRDKMGTDPKELSPF
                     HHVHRGMPPTIILHGKADSTVPYQTALLFANAMTDAGNQCRLVGYEGQAHGFFNYGRG
                     DGTHYQKTLDELDKFLVALGYLPPAK"
BASE COUNT          787 a         1391 c         1330 g          724 t
ORIGIN      
        1 catggtgccc acgctccgcg gcaattccag ccagcaaaag cagcacgact ttctgtattg
       61 gaccttctac gagcgcggcg gcggacgggc gatccgtgcg ggcgattgga agctggtcca
      121 gcagccgctg agctcgtccg agcggctcta caacctggcc caggacatcg gcgaggagcg
      181 cgacctggcc gctgagcaac ggcagcttgt cgcccagctg acgactaaaa tggactcggc
      241 ctatgtgccg cacgagaact ggaagttccc cgcgccgggc gcggccaagg ccaaaaccgg
      301 cgggaaacaa aaagcgaagt cgccgcaata gccagctatg aaaacctatt cgcattcggg
      361 ttgggccccg ccgcacggag tgttcgccac gctgtttgcc ggcatggcgg cggccgtgcc
      421 gtttggattc atctacgcct attcgttccg ctacatcccc tatccctatc tcaacattgt
      481 gatcacgctg gccttcggcg ctggcctggg ctttatcgtc gcgaagacgg ccgagtgggg
      541 caaagtccgc aacaacatca tcgtcggcgc gatcggtctc ttcgtcgggc tgttcggcct
      601 gtacgtctat tggggcgcgt attggtgggc cgtggcaggc ttcgatcggg tcgcgcagat
      661 cgggctctac gcctttatgc cctggggcat cctcgatctg gccctgaggt tgttcgacaa
      721 gggttcctgg ggaatttccg aaaacgcggg caacctcacg ggctggctcc tcgtcggcat
      781 ctgggtcatc gaaacctgta tcgtgctcgg actgtcggtg gtcgtctcgc tgctcaattc
      841 cgaccggcca ttttgcgaga cctgccaggt ctggaccgag tcgcagcgcg gcgtcgcccg
      901 cctgtcgggc aacggcagcg agcccgcctg ggaaaaggtc ctcgctggcg aattgcccgc
      961 gctggccgag tttccgcccg ccccgccagg ggcgatgcag tacgtacggt tggacctggc
     1021 ccgctgcccg cgctgcgaat acggccgttt cctcacgatc tgcgccgtgg acgtcgtggt
     1081 ggacaaggaa ggcaaggcct cggaaaaggc gcgggccctg gtgaccaacg gcatcgtcac
     1141 gccgtcgcag tgcgccgtgg tcgaagcctg cggcaagctg tacgaccagc acctcgaaca
     1201 gagtttaggg ccactctccg cggaggcagg cggcccgccc gcaccagccg aaggagaatc
     1261 gtgatggctc cgttccgctt gccctcctgc gcggcgatcg ccctggcgat gctggcaacc
     1321 cttgctgagg cggcggagcg gcccaacttc gtcgtcttgc tctgcgacga cctgggctac
     1381 ggcgacctcg gctgctacgg ccatccgcaa atcaagtcgc cgcggctcga caagctggcg
     1441 accgaaggca tccgcttcac cgagtgctac gcggccgcgc cggtctgcag tccctcgcgg
     1501 gccgggctgc tcaccggccg caatccgggt cgggccggca tctacgactg gatctcgaac
     1561 aaccacccga tgcacctggg cgcgaacgag gtgacgatcg ccacgctgct caaacaggcc
     1621 ggctacgcga cctgccagat cggcaagtgg cacctcaacg gcaaattcaa tcaggccgat
     1681 cagccgcagc ccggcgacca cggatttcag cactggatga gcacgcagaa caacgccggc
     1741 ccgtcgcacg ccaatccgcg gaacttcgtc cgcaacggca cgccgctggg cgatcagcag
     1801 ggctattcgt gcgacctggt gacggcggag gcaatccgct ggctcagcga cctgcgcgac
     1861 aagggcaagc cgttcttcct ctacgtctgc taccacgaga cgcacgagcc ggtggcctcg
     1921 cccgcggacc tcgtggccca gtatcccgac gccaagaagc cgggcgaagc cgagtactac
     1981 gccaacgtcg ccaacgtcga tcgggcgatc ggccggctgc tcgacaagct cgacgagttg
     2041 aagctctccg agcggacgct ggtgttcttc acttccgaca acgggccgga gacgctgctg
     2101 cggtacgcta gcggcaagca ctgccacggc tcgcccggtg cgctgagggg catgaagctg
     2161 cacctctatg aaggcggcat ccgcgtgccg ggcatcctcc gctatccgac gcgagtcaaa
     2221 ggcggccaga ccatcgacga accggtgtgc agcctcgatt tcctgccgac attctgcgag
     2281 ctgggtggag tgaagctgcc cgaaggccgg ccgctggacg gggcgagctt cacgccgctc
     2341 tttgacgggc agccgattgt gcggacgacg ccgctctact ggcaatactt ccgctcgatc
     2401 ggcgagccga aggcggcgct gcgcgtcggc gactacgtga tcctcggcaa gtgggacggc
     2461 ccgcagctgc cgcccggcgc gggcctggct cacggcgact gcgagatcat caaggccgcg
     2521 aagctgagcg ggttcgagct ctacaatctc aagaccgacc ccggccagaa gaccgacctt
     2581 gccgctgccg aacccggcaa gctcaaggag ctctccgacc tgctcgtcgc caagtaccag
     2641 gaagtccaga aggaagggcc agtgtggaag gtgcccaagc cggttgggaa ggagaaatag
     2701 ccgtaatgta ggcctccccc ggatcggtgc cctgaacatg cccctggcgc gccgatttcg
     2761 ccacaaggcc ttgccagaca gtgacttgtg acgcggtgcc gaaaagccca taagtagtta
     2821 tatattaggc tttgagacgg gtcattcgcg attttgcgcg aggccgcggc gcgccaaaat
     2881 ggcagccctc cgcgccaact gaaactgtag agcaaatagc gtaccaatgt acacaaatcg
     2941 ctcacgctgg gaatcgagtc gctctttttg ggagcattgc atttctggtt cccaagctcc
     3001 gcttgggaac ccacacacca aagctccgct tctcaagcag gaagcagagc ttccgcctcc
     3061 cgccgctccc aagcggagct ggggagcgag atactccagg agtattgcat gcgacgcaca
     3121 ctcactgtct tgacttgcac gtttctcgcg ttcgcttcaa gcgccctcgg ccaggaaaaa
     3181 gccggccgca ggtatccgcc ggatttgccg ggggcgaagg tcgtgaccta caagacggtc
     3241 ggcgatacga agctcaacct ctggatctat gaaccggaag ggcacaagcc ggaggacaag
     3301 tcaccggcga ttgtgttctt ctttggcggc ggctggacgg gcggctcgcc ccagcaattc
     3361 gagcagcaat gcaagcacct ggcgggccgc gggatggtgg cgatgaccgc cgactatcgc
     3421 gtcgccagcc gcaaccaggt caaggcggtc agttgcgtgg ccgacgccaa gagcgcggtc
     3481 cgctacatcc gccagaacgc cgcgcggctg ggcatcgatc ccagtcgcat cgccgccggc
     3541 ggcggctccg cgggcggaca ccttgccgcc tgcaccgccg ccctcaaggg cttcgatgaa
     3601 gccggtgaag acgcgtcagt cagcagcgtc cccaatgcgc tggcgctatt caatccggcg
     3661 gtcgtgctcg cggcggtgga cggcatccgc gagctcgacg cggccaaagt cagttccttc
     3721 cgggacaaga tgggcaccga tccgaaggag ctttcgccct ttcaccacgt ccaccgcggc
     3781 atgccgccga cgattatcct ccacggcaag gccgactcga ccgtgcccta ccagaccgcc
     3841 ctgctgttcg ccaacgccat gaccgacgcc ggcaaccagt gccggctcgt cggctacgaa
     3901 ggccaggccc acggcttctt caactacggc cgaggcgacg gcacgcacta tcagaagacg
     3961 ctggatgaac tcgataagtt tctcgttgct ctagggtatc tgccgccggc gaagtagagc
     4021 cccgagccgc tgcaacccaa acctcccaat ctgccgaatt tgacttccgc ggattgacag
     4081 cacgaagaac ggctcagtac gctagcaccg gcaactaccc acaattggtt gtgtttggtg
     4141 caggtttgag ggaagcgaac tcggcagagg tgcggcatgc gtcagcactt ggcagctcgt
     4201 cgtttgagtt gggtttgggc atgtctggtc tt
//
LOCUS       DASRJB010000596         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1861189, whole genome shotgun sequence.
ACCESSION   DASRJB010000596 DASRJB010000000
VERSION     DASRJB010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861189"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1686)
                     /gene="groL"
                     /locus_tag="VFB80_15735"
     CDS             complement(7..1686)
                     /gene="groL"
                     /locus_tag="VFB80_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698943.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZN35280.1"
                     /translation="MAKQMVFDDDCRQPLLAGASKLARAVRSTLGPRGRNAVLDKGWG
                     SPKVTKDGVTVAEDIELDDPFENLGAQLVKEAASKTNEVAGDGTTTATILAEAIFREG
                     LKMIASGYDPMALGRGIAKATDAVCEEVNKLSTPISEKNKKELEQIATIAGNNDPSIG
                     EVLADAFLKVGKDGVITLEEGKGSETTVEVVEGMQFDRGFLSPHFVTNQDDVSVELEN
                     AYILLYEEKISSNKKLIPLLEAISKANKPLLIVAEDIEGEALATLVVNKMRGILQVCA
                     VKAPGYGDRRKAIMGDIGVLTKATPIFKDLGIELESVKLSDLGRAKKIKVTSEDTIII
                     EGAGSKEDIAGRAQQIRDEIERTDSEYDREKLQERLAKLAGGVAQINCGAATETEMKE
                     RKWLLEDAKNAVQAALESGVVPGGGVALIRAEKAIDKLKLEGDEGAGARIIKNVLDQP
                     LRAIANNAGVDGAVVVNRVRQLKSKTEGYDADKDLYGDMLKFGIIDPAKVVTVALQNA
                     ASVASLLLTTEALVAEIPKEKDDEGGHHDHHDHGMGGMGGMGGMGGMEGMM"
     gene            1974..2336
                     /locus_tag="VFB80_15740"
     CDS             1974..2336
                     /locus_tag="VFB80_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35281.1"
                     /translation="MVQTLSTCRRTTTLAAVALILSANLHCAWEWQAALIYARQAPIE
                     HGCENESGCICRGALVAQPLDAAFLTSDSSWLLLELPPPILNGEIVAADSDCPIVHCL
                     APPISGRQLRALYASLVI"
     gene            2426..3916
                     /locus_tag="VFB80_15745"
     CDS             2426..3916
                     /locus_tag="VFB80_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN35282.1"
                     /translation="MSARTSFLATWPGLVRYSNFVIGGAIVAGGLVLLIALRLGWIEI
                     LPEGSQVAAETAVEPAVAPTSVQLTEEKLAAAGLHVALAASEPIQATRPVPGTIAYDA
                     TRRVPVSAPVSGVVTKVLVEPGQQVSEQQPLAEISSPEVGLARDEVLRHQADLELAKK
                     QLTFSEQIAANVESLLALLKQKPKLEELEASLAALTLGEYREKVVAAYSKLVLAERTL
                     ALSGTLEKGTLSQRLLDERRSNREVAAAQFSAACETARFAAALDRDKAKAAAEQAERL
                     LAVAQQALANLLGPLADMTPPADRTRLSELVLLAPIAGRVEERNAVAASRIAAGAPLF
                     ILADTTNLWVSGEIHERDWASLDVVSGREVRIRIPALNDTELPAKVRFVGNQVSPETR
                     SVPLVADLPNPDGRLKPGLFVWALVPLEKPRPALVVPSGAIMRHENQPFVFVPAGERN
                     YRRVDVEVGLDAGDRVEILSGLKAGEAVIDRGAFFLKSELLLEREE"
     gene            3947..>4239
                     /locus_tag="VFB80_15750"
     CDS             3947..>4239
                     /locus_tag="VFB80_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN35283.1"
                     /translation="MLTALIDLSLNNRFLVIALFCLLAIGGAYSATQIPIDAVPDMTN
                     TQVQVLTEAPGLSPIEVERLVTNRVENAMAGLPELTELRSTSKFGISAVTLVF"
BASE COUNT          677 a         1395 c         1293 g          874 t
ORIGIN      
        1 tcctgcctac atcatccctt ccatgcctcc cattccaccc atgccaccca tcccgcccat
       61 gccgtggtcg tgatggtcgt ggtggccgcc ctcgtcgtcc ttctccttgg gaatctcggc
      121 gaccagggct tcggtggtca gcagcagcga ggcgacgctg gcggcgttct gcagagcgac
      181 ggtcacgacc ttggccgggt cgatgatgcc gaacttcagc atgtcgccgt acaggtcctt
      241 gtccgcgtcg tagccttcgg tcttgctctt gagctggcgg acgcggttga caacgaccgc
      301 gccatcgacg ccggcgttgt tggcgatcgc ccgcagcggc tgatcgagga cgttcttgat
      361 gatccgcgct ccggcccctt cgtcgccttc gagtttgagc ttgtcgatcg ccttctcggc
      421 gcgaatcagg gccacgccgc cgccgggaac gacgccgctc tcgagcgcgg cctgcacggc
      481 attcttggcg tcttcgagca gccacttgcg ctccttcatt tcggtttcgg tcgccgcgcc
      541 gcaattgatt tgggcgacgc cgccggccag cttggccagc cgctcctgca gcttctcgcg
      601 gtcgtactcg ctgtcagtcc gctcgatttc atcgcgaatt tgctgggccc ggccggcgat
      661 atcctctttg cttcccgcgc cttcgatgat gatcgtgtcc tccgaggtga ccttgatctt
      721 cttggcccgg cccagatcag acagcttgac gctctccagc tcgattccca ggtccttgaa
      781 gatcggcgtg gccttcgtga gtacgccgat gtcgcccatg atcgccttgc ggcggtcgcc
      841 gtagcccggg gccttcaccg cacaaacctg caaaatcccc cgcattttat tgacgacgag
      901 cgtcgccagg gcctcgcctt cgatgtcttc ggcgacgatc agcagcggct tattggcctt
      961 gctgatcgcc tccaaaagcg ggatcagctt cttgttcgaa gaaatcttct cctcgtagag
     1021 caggatgtag gcgttctcca gctcgacgct cacgtcgtcc tggttcgtga cgaaatgcgg
     1081 cgaaaggaag ccgcggtcga actgcatgcc ttcaacgacc tcgacagtcg tctcgctccc
     1141 cttgccctct tcgagcgtga ttacgccgtc tttgccgacc ttgaggaagg cgtcggcgag
     1201 cacttcacca atcgacggat cgttgttgcc ggcgatcgtg gcgatctgtt ccagctcttt
     1261 cttgttcttc tcgctgatcg gcgtcgaaag cttgttgact tcctcgcaga ccgcgtcggt
     1321 ggctttcgcg atcccgcggc cgagggccat cgggtcatag cccgacgcga tcatcttcag
     1381 cccttcgcga aaaatcgcct cggccaggat cgttgccgtc gtcgtgccgt cgcccgccac
     1441 ttcgttggtc ttgctggcgg cttccttgac gagctgagcc ccgagatttt cgaagggatc
     1501 gtccagttcg atgtcttcgg cgaccgtcac gccgtccttc gtcaccttcg gcgagcccca
     1561 gcccttgtcg agcaccgcgt tacggccgcg ggggccaagc gtgctgcgga ccgcacgggc
     1621 cagctttgac gctccggcca agagcggctg ccggcagtcg tcgtcaaaga ccatttgctt
     1681 tgccatgctt gagaatcctc ctgttgccga tgaaagtgtg cttaagggtc gcgccggagt
     1741 taagtccgaa ccgtcagcga ccaaactggc agtctggggc gtagagccgc tcagaattgc
     1801 aagcgatgtg ccaagccagc aaactggaga cataacctgt ttctggcaaa cgacttgcag
     1861 aacacttcgt cagtcgaatt ctcaccaccc ggctgatagc ttgtcatttt ggcagcgtgc
     1921 gtattccgac cgcgatttgc gttcgacctg agggctggct attgtgacgg gcgatggttc
     1981 agacgctctc cacctgccgc cggacgacaa ccctggcggc cgttgcactg atcctaagtg
     2041 cgaatctgca ttgtgcctgg gagtggcagg cggccctgat ttacgcacgc caggcgccaa
     2101 tcgagcacgg ctgcgagaac gaatcgggct gcatctgccg cggcgccttg gtagctcagc
     2161 ctttggacgc cgcttttctc accagcgatt ccagctggct gctgctggag ttgccgccgc
     2221 cgattttgaa tggcgagatc gtggcggcgg atagcgattg ccccatcgtt cactgtcttg
     2281 cgcccccaat ttccgggcgg caactgcgcg cgctctatgc gtcgctggtg atctagtcgc
     2341 gctattctgc gctgcccttg cgctgcggat gcgggcattc cgcccgcgtg ttcccttcgc
     2401 tcgctgaaat ggctgccgtt ttcccatgag cgctcgcact tcctttcttg cgacctggcc
     2461 gggccttgtc cgctattcca actttgtcat cggcggcgcg attgtcgcgg gcggcctcgt
     2521 gctgctaata gcgctgcggc tgggctggat cgaaatcttg ccggagggca gccaggttgc
     2581 ggccgaaacg gcggtcgagc cggctgtcgc gccgacgagc gtgcaactga cggaagaaaa
     2641 gctcgccgcg gcgggattac acgtggcgct ggccgccagc gagcccattc aggcgacgcg
     2701 gccggttccc ggcacgatcg cctacgacgc cacgcggcga gtgccggtga gtgctccggt
     2761 cagcggcgtc gtgaccaagg tgctcgtcga gcccggccag caagtctccg agcaacagcc
     2821 gctggccgaa atctccagcc cggaggtcgg cctggcccgc gacgaagtgc tgcggcatca
     2881 ggcggacctg gagttggcga aaaagcagct cacgttttcg gagcagattg cagccaacgt
     2941 cgaatctctg ctcgcgctgc tcaagcagaa gccaaaactc gaggagctcg aagcatcgct
     3001 cgcggcgctg acgctgggcg agtatcgcga gaaagtcgtc gcggcttatt ccaagctcgt
     3061 gctggccgag cggacgctcg cattgtcggg cacgctcgaa aagggcacgc tcagccagcg
     3121 tctgctcgac gagcggcgga gcaaccgcga ggtggccgcg gcccagtttt ccgccgcgtg
     3181 cgagacggcc cgcttcgccg ctgctctgga tcgtgacaag gctaaggccg ccgccgagca
     3241 ggccgagcgg ctgctggccg tcgcgcagca agccctggcg aacctgctgg gcccgctggc
     3301 cgacatgact ccgccagccg accgcacaag gctcagcgag ctggtgctgc tcgcgccgat
     3361 cgccgggcga gtcgaagagc gcaacgccgt tgcagcttca cgcatcgccg ccggtgcgcc
     3421 gttattcata cttgccgaca cgaccaacct gtgggtttcc ggcgaaattc acgaacgcga
     3481 ttgggcctcg ctcgacgttg tctcaggacg agaagtccgg atccgcattc ccgccctgaa
     3541 cgacacggaa ttgcccgcca aagttcgatt cgtcggcaat caggtctctc ccgaaacccg
     3601 cagcgtgccg ctggttgccg atctgcccaa tcccgacggc cgactgaagc cgggtttgtt
     3661 cgtctgggcg ctcgtgccgt tggaaaagcc gcggccggcg ctcgtcgtgc cctccggcgc
     3721 gatcatgcgg cacgagaatc agccgttcgt atttgtcccg gcgggcgagc gcaactaccg
     3781 gcgcgtcgat gtcgaggtcg gcctcgacgc tggcgatcgc gtcgaaatcc tctccggcct
     3841 caaagccggc gaagccgtaa tcgatcgcgg ggcgtttttc ctgaaatcgg aactgctgct
     3901 cgaacgagaa gaatgactcc gactcccgac tcttgactcg acgcccgtgc ttaccgccct
     3961 catcgatttg tcgctcaaca accgcttcct ggtgattgcg ctgttctgct tgctggcgat
     4021 cggcggcgcg tatagcgcca cgcaaattcc gatcgacgcg gtgccggaca tgaccaacac
     4081 gcaggtgcaa gtgctgaccg aagcgccggg cctgtcgccg atcgaggtcg aacggctggt
     4141 caccaaccgc gttgagaatg cgatggcggg actgcccgag ctgaccgagc tgcgcagcac
     4201 atcgaagttc ggcatctctg cggtcacgct cgtattcca
//
LOCUS       DASRJB010000597         8226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3274386, whole genome shotgun sequence.
ACCESSION   DASRJB010000597 DASRJB010000000
VERSION     DASRJB010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274386"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFB80_15755"
     CDS             complement(<1..824)
                     /locus_tag="VFB80_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HZN35284.1"
                     /translation="MTTSKLSRPLFDPPIVKRALWEAVVKLDPRIQLRNPVMFVVLVG
                     AAATLLLLVERLAAGSGESPWFVAGVSLWLWFTVLFANFAEAMAEGRGKAQADALKAA
                     RQNVAAKKLLADVGSPKVPLDFQFLVKQGEATFVPAPDLRQGDVVLVEAGDQIPGDGE
                     VIAGVASVDESTITGESAPVIRESGGDKSSVTGGTRVLSDWLIVRITANPGESFLDRM
                     IGLVEGAKRQKTPNEIALSILLSGFTIIFLLVCATLLPYSLFSVGEAKRLDPGSLAA"
     gene            complement(941..2674)
                     /gene="kdpA"
                     /locus_tag="VFB80_15760"
     CDS             complement(941..2674)
                     /gene="kdpA"
                     /locus_tag="VFB80_15760"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZN35285.1"
                     /translation="MRMGILQLVVYLVVLVLLVKPLGWYMARVYQGQPYGLDRLLGWL
                     ERGLYWLAGTRPDAEMTWRQYALAMLLLNLVGLAAVYGLQRFQHLLPLNPEQLGAVTP
                     DSSFNTAVSFATNTNWQGYGGETTMSYLTQMLGLTVQNFVSAAAGMATLVALIRGFTR
                     KEASTIGNFWVDLTRSTLYILLPLSLVLAIVLVSQGVVQTFSPHVTATLIEPTTDADG
                     KPVTEQAIALGPAASQIAIKQLGTNGGGFFNVNSAHPLENPTPLSNFLELLAILLIPA
                     ALCYTFGEMVGDRRQGWALLAVMLVLFVPCLAVCYLSEQGGNPRLTAAGVDQTSGSLQ
                     AGGNMEGKEVRFGIANSALWATATTAASNGSVNSMHDSFTPLGGFVPMWLMQLGEVIF
                     GGVGSGLYGLLAFAIVAVFVAGLMVGRTPEYLGKKIEAYEMKMASLIILIPCAVVLIG
                     TAVAVVTPEGKATILNPSAHGFSEVLYAFSSAGNNNGSAFAGLGANTPFYNTALGIAM
                     FAARYWLAVPILAIAGSLARKRIVPASSGTLPTHTPLFVVLLASVVVIVGALTFLPAL
                     ALGPIVEHLQL"
     gene            complement(2674..2784)
                     /gene="kdpF"
                     /locus_tag="VFB80_15765"
     CDS             complement(2674..2784)
                     /gene="kdpF"
                     /locus_tag="VFB80_15765"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZN35286.1"
                     /translation="MRRPGGRTHMIYAIAAALAVLLLVYLTVSLLKPEWF"
     gene            complement(2942..3439)
                     /locus_tag="VFB80_15770"
     CDS             complement(2942..3439)
                     /locus_tag="VFB80_15770"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZN35287.1"
                     /translation="MRVEFINPFVKATVQVFRSRLACELVRRQLLRKTGRQPHQEVSG
                     IVELSGQAHGAVVFGLSRPLAMHVTQVLRQASVETLNEQVQGAVVELVQLVAAAARVE
                     LAQLELQVGPPRVIIGRHHTLPLASDGDSLDIVFESPWGIIVVEVGLSASEHASQTAG
                     AASAS"
     gene            3800..6196
                     /locus_tag="VFB80_15775"
     CDS             3800..6196
                     /locus_tag="VFB80_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN35288.1"
                     /translation="MRRTASPLHLLLVGLMIGLTGCHPTQPFYLKEDGDLSHYIDAAT
                     TADTPDVHQAPLPEVEHAHSPLTLSHPDFKEFWELSLEECVAITLANSKILRGGNSAR
                     LQNGQLFPGVQQGSRITNPGGLFSTMYDPAVVESNPGQANGAVPGFLQSGAGSAFNGP
                     SVDGGVANVRQGVEAALAEFDAQLNITGTGPSGSIASSTDRPQNVDINAFGFPTILDL
                     RNGGLQATLAKRTAEGSLVSFSSTTDYNRGISRGDIQALNSTWTQVLQVDVRHPLMQG
                     RGAQINRMPVILARMGTDITIMNLMGALQDTLNNVEIYYWDLYLAYRNLETAKVGRDS
                     ALGTWRIVYEKFKQEVEPVQAEAQAREQYFSFRAAVETSLRNLYDTENNLRLLMGLAA
                     TDGRLIRPKDEPSLARVEFEWCDILAEAIARRPELIQQRWRIKTREMELILARNLLLP
                     RLDVGAQYRWLGIGDDLIFAGRTGVDFPLAGSTAFEEITSGRFQEFSFLFQYDMPIGF
                     RRELAGVRHAQLRLAREKAFLEDIELDVSHGLALSVRNVDTNFHLAQTNANRWAASQE
                     DVNAREALYKAGRVALDDVLEAQRRRAVAQSAFWQSVIEYNKSIADLHTRKGSIMEYD
                     GVSFGEGPWPEKAYWDALARARERDAGHYIDYGWTRPKVISRGPIPEHAPGIGSFHLE
                     NPDGAALEQLPAAEPTPAAPPDGGSGILPAPTPRTVPGALEARAPQPLSATPQLAVPD
                     EPTPAALPAPPGGSVRKAAANLAPASFSSTNPLRINPAQPIGTGVADPPARAALPAGN
                     "
     gene            complement(6442..>8226)
                     /gene="sppA"
                     /locus_tag="VFB80_15780"
     CDS             complement(6442..>8226)
                     /gene="sppA"
                     /locus_tag="VFB80_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZN35289.1"
                     /translation="KPDSAKAAETTKQIVAVFRLKGGLTETPTDESFPLMLDQVTALK
                     SLVERMQKAAADKDVKAVVITLNDFGAGFAQIEELRQAIAGLRSAGKDVYAHSDSLQM
                     PDYLLLCGATRLSVVPTGDLWLTGLSGESLHVRKLLDLIGAKPDFLTCGAYKSAAEMF
                     MRAEPSKEADEMHNWLLDSLYDSSVKLIASGRGVQPEKVRKWIDDGPYTAEKAKQAGL
                     IDSVEHRQDFEAGVKQRLGDKVTFDTKYGKAAQQQIDLSSPFGIINFYSELLSGGKKA
                     KSHKESVAIVYVEGPIVLGAPTPSLFDAGGGIAASTPIRAALNKAAADDSIKAVVLRV
                     NSPGGSAVASEIILDATKRVKAKKPLVVSMGSVAGSGGYYVACGSDTIFADECTITGS
                     IGVVAGKLATQEMFGKVGVTWKEYNRGANAALLSSSNVFTEAERKKLQGWMDDIYGVF
                     KGHVTAIRGSKLKKPIDDLAGGRVYTGKQALELGLVDKLGTLDDAVKFVAKEAKLDKY
                     ELRIVPEPKSFIEQILESSSGDKDEDTVGIGAAPFSTGSASLLDSALPYLEHLDRRHC
                     QAVLRALTQLDILHREGIALVTPEVIVH"
BASE COUNT         1421 a         2829 c         2642 g         1334 t
ORIGIN      
        1 gcggccaggg aacctggatc gagcctcttc gcctcgccaa cgctgaacag cgaatagggc
       61 agcagcgtgg cgcagacgag caggaagatg atcgtgaatc cggacagcag gatgctgagg
      121 gcgatttcgt tcggggtctt ctggcgcttg gcgccttcga ccaggccgat catccggtcg
      181 aggaaactct cgccggggtt ggcggtgatg cggacgatca gccagtcgct gagcacgcgg
      241 gtgccgccgg tgacgctgct tttgtcgccg ccgctctcgc ggatgaccgg agcgctttcg
      301 ccggtgatgg tgctttcatc gaccgaggcc acgcccgcga tcacctctcc gtcgccgggg
      361 atctggtcgc cggcttcgac gagcacgacg tcgccctgcc gcagatcggg cgcgggcaca
      421 aacgtcgcct cgccctgctt gacgaggaac tggaaatcga gcggcacctt gggagagccg
      481 acgtcggcga gcagcttttt ggcggccacg ttctgccgcg cggctttcag ggcgtcggcc
      541 tgggctttgc cgcggccctc ggccatcgct tccgcgaagt tggcgaagag caccgtgaac
      601 caaagccaga ggctgacgcc cgccacgaac cacggcgact cgccgctgcc cgcggccaga
      661 cgctcgacca gcagcagcag cgtggccgcc gcgccgacga gcaccacgaa catcacgggg
      721 ttgcggagtt gaatccgcgg atcgagcttg acgaccgcct cccacagcgc ccgcttcact
      781 atcggcgggt cgaacagggg tcggctgagt ttgctggtgg tcattggtca cttgtcattg
      841 gtcacttgtg tagggtgggt caagcgagcc tggcgagcga agacccacgc gggttcaagt
      901 ttgccgcgtg ggtctaaggc cgcggcggcc ttgacccacc ctacagctgc aggtgttcga
      961 cgatcggccc gagggccagc gccggcagga acgtcagtgc gccgacgatg acgacgacgc
     1021 tggccagcag cacgacgaac agcggcgtgt gcgtggggag cgtgccgcta gaggccggca
     1081 cgatcctctt gcgggccagc gagccggcaa tcgccaggat cggcacggcc agccaatagc
     1141 gggccgcgaa catcgcgatg ccgagggccg tgttgtagaa gggggtgttg gctcccaggc
     1201 cggcgaaggc actgccgttg ttgttgcccg cgctggaaaa tgcgtacagc acttcgctga
     1261 agccgtgggc cgacggattg aggatcgtcg ctttgccctc aggagtcaca accgcgaccg
     1321 ccgtgccgat cagcacgacg gcgcagggga tcaggatgat gagcgaagcc attttcattt
     1381 cgtaggcttc gatcttcttg cccagatact ccggcgtgcg gccaaccatc aggcccgcca
     1441 cgaagaccgc gacgatggca aaggcgagca aaccatacag gccgctgccg acgccgccga
     1501 agatcacctc gcccagctgc atcagccaca tcggcacgaa gccgcccagc ggcgtgaacg
     1561 agtcgtgcat cgagttgacg ctgccgttgg aggccgcggt tgtcgccgtc gcccacagcg
     1621 ccgaattggc gatcccaaac cgcacctcct tgccttccat gttgccgccg gcctgcaggc
     1681 ttccggacgt ctgatccacg cccgcagccg tcagccgcgg attgccgccc tgctccgaca
     1741 ggtagcagac cgccaggcag ggcacgaaca ggaccagcat cacggccaac agcgcccagc
     1801 cttgccggcg gtcgccgacc atctcgccga acgtgtagca caaggccgcg ggaatgagca
     1861 ggatcgccag cagctccaga aagttcgaga gcggcgtcgg attctccagc ggatgggccg
     1921 agttcacatt gaagaagccg ccgccgttgg tgcccagctg cttgatcgcg atctgcgaag
     1981 cggccgggcc aagggcgatg gcctgctcgg tgaccggctt gccgtccgca tccgtcgtcg
     2041 gctcgatcaa tgtcgccgtg acatgaggcg agaaggtctg caccacgccc tggctgacca
     2101 gcacgatggc caggaccagc gacagcggca gcaggatgta gagcgtgctc cgcgtcagat
     2161 cgacccagaa gttgccgatc gtgctggctt ccttgcgagt gaagccgcgg atcagcgcca
     2221 cgagcgtcgc catgccggcg gccgccgaga cgaagttctg caccgtcagg ccgagcatct
     2281 gcgtcaggta gctcatcgtc gtctcgccgc cgtagccctg ccagttcgtg ttcgtggcaa
     2341 agctcacggc cgtgttgaac gacgaatcgg gcgtgacggc cccgagctgc tcggggttga
     2401 gcggcagcag gtgctggaac cgctgcaacc catacacggc cgccaggccg accaggttca
     2461 gcagcagcat cgccagcgcg tactgccgcc aggtcatctc ggcatccggc cgcgtgccgg
     2521 ccagccaata cagtcctcgc tccagccagc ccagcaggcg gtccagcccg tacggctgtc
     2581 cctgatagac gcgggccatg taccagccca gcggcttgac gagcagcacg agcaccacca
     2641 agtacacgac gagttggaga atgcccatcc tcatcagaac cactccggct tcaggagcga
     2701 gacggtcagg taaacgagca agagcaccgc cagcgcggcg gcaatcgcgt agatcatgtg
     2761 cgttctccca ccagggcgcc gcacagcccg atcaatccga ccgtcagggc cgcgagcagg
     2821 gccacgattc ccagaaagac agcgtccatt gtgcgagcct ccgcagcagg tatctgcgga
     2881 atgctatccg gacgcgcatc aagatgccgt caaaaccggc cggccgcgcg ttaagaaatc
     2941 gtcaggaggc gctggccgcg cccgccgtct ggctggcgtg ctcgctcgcc gacaggccga
     3001 cttcaacgac gatgatgccc caggggctct cgaagacgat gtccagcgaa tcgccgtcgc
     3061 tggccagcgg cagcgtgtga tggcggccaa tgatcacccg cggcgggccg acttgcagct
     3121 ccagctgcgc gagttccacc cgcgcggcag cggccaccag ctgcaccagc tcgaccacgg
     3181 ccccctgcac ctgctcgttg agcgtctcga ccgacgcctg ccgcaggacc tgcgtgacgt
     3241 gcatcgccag cggccggctg agaccaaaga ccaccgcccc gtgcgcctgt cccgacagct
     3301 cgacgatgcc gctgacttcc tggtgcggct gccggccggt cttgcgcagc agctgccgcc
     3361 gcaccagctc gcaggcgagc cggctgcgga agacctggac ggtggccttc acgaacggat
     3421 tgatgaattc gacgcgcatg gcagcaaggc ggagctagca ggtccccgca ctggaaatct
     3481 atggcgcggg gcgctctggc gcgcgttaat ttgggcagtg ccggttctgc ccgccttgtt
     3541 cagccgccgc ggcttcgcag cgattgtcgg tttgggcact ctggcgaggg agggcaaccc
     3601 gccatttgcc agaaatcccc gccaaagaaa tcgattggtc aaagtttacc ggggaggcag
     3661 tcgatatcaa cgctgctgat ttcccgactt ggggcgtgcg cctgcgccgc ccggcgaggg
     3721 taatcggtca ggggggcctg accgacggcc gcgattggag tacgcaatcg ccgccacacc
     3781 aaccaaggac gggaattcga tgcgccggac cgcctcccct ctgcatctgc tgctggtagg
     3841 gctgatgatc ggcctgaccg gctgccatcc gacgcagccg ttctatctga aggaagacgg
     3901 cgacctgtcg cactacatcg acgcagccac gacggccgac acgccggacg tgcaccaggc
     3961 cccgctgccg gaagtcgagc acgcccactc gccgctcacg ctcagccatc ccgacttcaa
     4021 ggaattctgg gagctctcgc tcgaggaatg cgtcgcgatc acgctggcca acagcaagat
     4081 cctccgcggc ggcaattcgg cccgcctgca aaatggtcag ctctttcccg gcgtccagca
     4141 gggctcgcgg atcacaaatc ccggcggttt gttttcgacg atgtacgatc cggcggtcgt
     4201 tgaatcaaac cctggccagg ccaacggcgc ggtgcccggc ttcctgcaat cgggcgcggg
     4261 cagcgcgttc aacggcccgt cggtcgatgg cggcgtggcc aacgtgcggc aaggcgtcga
     4321 ggccgccctg gccgagttcg acgcgcagct caacatcacc ggcaccggcc cctcgggctc
     4381 aatcgccagc agcaccgacc gcccgcagaa cgtggacatc aacgcattcg gctttccgac
     4441 catcctcgac ctgcgcaacg gcggcctgca ggcgacgctc gccaagcgga ccgcggaagg
     4501 cagcctggtc tcgttctcga gcaccaccga ctacaaccgc ggcatcagcc gcggggacat
     4561 tcaagccctc aacagcacct ggacccaggt gctgcaagtc gatgtccgcc atccgctgat
     4621 gcagggccgc ggggcgcaga tcaaccgcat gcccgtgatc ctggcccgca tggggaccga
     4681 catcacgatc atgaacctga tgggcgctct gcaggacacg ctcaacaacg tcgagatcta
     4741 ctactgggac ctgtacctcg cctaccgcaa cctggagacg gccaaggtcg gccgcgacag
     4801 cgccctgggc acctggcgga tcgtctatga gaagttcaag caggaagtcg agccggtcca
     4861 ggccgaagcc caggcccgcg agcaatactt cagcttccgc gccgccgtcg agacctcgct
     4921 ccgcaacctg tacgacaccg agaacaacct gcggctgctg atgggcctgg ccgcgaccga
     4981 cggccgcctg atccggccga aggacgagcc ctcgctggcc cgcgtcgaat tcgagtggtg
     5041 cgatatcctg gccgaggcca tcgcccgccg gccggaactg atccagcagc ggtggcggat
     5101 caagacccgc gagatggaat tgattctggc ccgcaatctc ctgctgccgc ggctggatgt
     5161 cggcgcgcag taccgctggc tgggcatcgg cgacgacctg atctttgcgg gccgcaccgg
     5221 cgtcgatttc ccgctcgcgg gctcgacggc gttcgaagag atcaccagcg gcaggttcca
     5281 ggagttctcc ttcctgttcc agtacgacat gcccatcggc ttccgccgcg agctggccgg
     5341 ggtgcggcac gcccaattgc ggctggcccg cgaaaaggcg ttcctggaag acatcgagct
     5401 ggacgtctcg cacggcctgg ccctgtccgt ccgcaacgtc gatacgaact tccacctggc
     5461 gcagaccaac gccaaccgct gggccgcgtc gcaagaagac gtcaacgccc gcgaggcgct
     5521 ctacaaggcc ggccgggtgg ccctcgacga cgtgctcgaa gcccagcgcc gccgggccgt
     5581 cgcccagagc gcgttctggc aatcggtcat cgaatacaac aagtcgatcg ccgacctgca
     5641 tacccgcaag ggctcgatca tggagtacga cggcgtctcc ttcggcgaag gcccctggcc
     5701 ggaaaaagcc tactgggacg ccttggcccg cgctcgcgag cgcgacgccg gccactacat
     5761 cgactacggc tggacgcggc ccaaggtcat cagccgcggc ccgattccag agcacgcacc
     5821 gggcatcggc agcttccacc tggagaaccc cgacggcgcg gccctcgaac agctgcccgc
     5881 cgccgagccg acgcccgctg ctccgcccga tggcggcagc gggatcctgc ctgctccgac
     5941 tccgcgaacg gtgcccggag cgctcgaagc ccgcgctccg cagcccctga gcgcgacgcc
     6001 ccagctggcg gttcccgatg aaccgacgcc ggccgcgctg cccgctccgc ccggcggcag
     6061 cgtgcggaag gccgcggcca acctcgcccc cgcctccttc agctccacca atccgctgcg
     6121 catcaacccc gctcagccga tcggcaccgg cgtcgccgat ccgcccgccc gcgccgcgct
     6181 gccggcgggc aactagcggc cgcttagccc cggcccccaa gccggctgac tgccagcgcc
     6241 gctaggcgcg ccgaacgcga cgggcggccg agccggatga agattaggat caggattaag
     6301 attaagaaga tgcgtaagcg caggatgaag agtaagagca ggattgctgc ccgcgctcga
     6361 tcgtaacctt catcctaatc ctactcttcc tcttactctt gctcccgccc cggtcgggcg
     6421 tgtgtttgga cctgggccga cctaatggac aatgacctcc ggcgtcacca gcgcgatgcc
     6481 ctcgcggtgg aggatgtcca gctgggtgag ggcccgcagg acggcctggc agtggcggcg
     6541 gtcgaggtgc tccaggtagg gcagcgccga atcgagcagg ctcgccgagc cggttgaaaa
     6601 gggcgcggct ccgatgccga ccgtgtcttc gtccttgtcg ccgctgctgc tttccagaat
     6661 ctgctcgatg aagctcttgg gctcgggcac gatccgcagc tcgtacttgt cgagcttcgc
     6721 ctctttggcg acgaacttga cggcgtcgtc gagcgtgccc agcttatcga ccaggcccag
     6781 ctccagtgcc tgcttgccag tatagacgcg gccgccggcc aggtcgtcga tcggcttctt
     6841 cagcttgctg ccgcggatcg cggtgacgtg ccccttgaag acgccgtaga tgtcgtccat
     6901 ccagccttgc agcttcttcc gctcggcctc ggtgaagacg ttcgacgagc tgagcagggc
     6961 ggcgttcgct ccgcggttgt attccttcca ggtgacgccg accttgccga acatctcctg
     7021 cgtggccagc ttgccggcga cgacgccgat cgagccggtg atggtgcatt cgtcggcgaa
     7081 gatcgtgtcc gagccgcaag ccacgtagta gccgccgctg ccggcgacgc tgcccatgga
     7141 gacgaccagc ggcttcttgg ctttcacgcg cttggtggca tcgaggatga tctcgctggc
     7201 cacggcagag ccgccgggcg agttgacgcg caggacgacc gccttgattg agtcgtcggc
     7261 cgccgccttg ttcagcgccg cgcggatcgg cgtgctggcg gcgatgcctc cgccggcgtc
     7321 gaagaggctc ggcgtcgggg cgccgaggac gatcggccct tcgacataga cgatcgccac
     7381 cgactctttg tgcgactttg ccttcttacc gccgctgagc agctccgaat agaaattgat
     7441 gatcccgaac ggcgaggaga ggtcgatctg ctgctgggcc gccttgccgt acttcgtgtc
     7501 gaacgtcacc ttgtccccca gccgctgctt cacgcccgcc tcgaaatcct gccggtgctc
     7561 gaccgagtcg atcaggccgg cctgtttggc cttttccgcc gtgtaagggc cgtcgtcgat
     7621 ccacttgcga actttctcgg gctgtacgcc gcggccgctg gcgatcagct tcaccgagct
     7681 gtcataaagg ctgtccagca gccagttgtg catctcgtcg gcttccttgc tgggctcggc
     7741 ccgcatgaac atctcggcgg cgctcttgta ggccccgcag gtgaggaagt cgggcttcgc
     7801 gccgatcagg tcgagcagct tgcggacgtg caggctttcg ccggacaggc ccgtgagcca
     7861 caggtcgccc gtcggcacga cggagagccg cgtcgccccg cacaagagca gatagtcggg
     7921 catttgcagc gaatcggagt gggcatagac atccttgccc gccgaccgca gtcccgcgat
     7981 cgcctgccgc agctcttcga tctgcgcgaa gcccgcaccg aagtcgttga gcgtgatcac
     8041 gaccgccttc acgtccttgt ccgccgccgc tttttgcatc cgctccacga gactcttgag
     8101 tgcagtcacc tggtccagca tcaagggaaa actctcgtcg gtcggcgtct cggtgaggcc
     8161 gcccttcagc cggaagacgg cgacgatctg tttggtggtc tcggcggcct tggccgaatc
     8221 gggctt
//
LOCUS       DASRJB010000598         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2139053, whole genome shotgun sequence.
ACCESSION   DASRJB010000598 DASRJB010000000
VERSION     DASRJB010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139053"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1197
                     /locus_tag="VFB80_15785"
     CDS             106..1197
                     /locus_tag="VFB80_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN35290.1"
                     /translation="MSLAFPNFRPHPLIRGGHLQTVVGSYLPWLKVHYRARQHHVPLP
                     DGDQIVLHDDLPASPVSCSLSPVSRSDATWQPGDPVALLLHGLGGCHQSGYMQRCAVK
                     LAERGYRVFRMDLRGYGAGFPLARHPVHAGRSEDAAAALSYVIETCPDSPIHLVGFSM
                     GANIVLKLAGELGSQAPPHLASVMAIGPPIDLIECSKNMQRGWNRLYDRRFARNLVRF
                     IQQRSQVRPDALSRPLVPQPRSLLDFDAIFTAPLSGFADTDDYYTQASSGRLLPRITV
                     PTLIVTAASDPIIPVGPFERASYSPSTQLIITPCGGHLGFVGARGVDPDHRWLDWRIV
                     EWVASHAVRPAADPRSRPTAHSRDATPAL"
     gene            complement(1518..>2398)
                     /locus_tag="VFB80_15790"
     CDS             complement(1518..>2398)
                     /locus_tag="VFB80_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZN35291.1"
                     /translation="ELDPQLAAYRQQFLRAAAAVIAGRTAADKLEEITQIRENVLALA
                     KKDDLTKAEIVATSDPGLARLKELILVSRKDILDSNANLIAARNRLQVLGKEWELCAT
                     LILAAKPEPAKTDAAPQEKQPAEEIAELDTTPSFEKYLVKDEEIATALATPMSDAARR
                     TLAQNNQLAGQLDPEEARCVLDLNLTRNLLGLDPLVIDLKLAAAARDHSRDMEEQKFF
                     DHESPVPGKKTPWDRAEKFGTTASGENIALGTLDGAVVNQMWWHSPGHHRNMLGSHTR
                     VGLGRHNQHWTELFGR"
BASE COUNT          349 a          803 c          762 g          484 t
ORIGIN      
        1 ctgacagcac gctactaggg attgtcgtgg tgaatgattt tcaaggcggg gcttgaaacg
       61 ggggcggtca aaacgagtat aaagggagaa cggttgcccc cgcatatgtc gcttgccttc
      121 ccaaatttcc gcccgcatcc gctcattcgc ggcggccatt tgcagaccgt cgtcggctcg
      181 tatctgccgt ggctcaaggt ccactatcgc gcgcggcaac atcacgtccc gctgcccgac
      241 ggcgatcaaa tcgtcttgca cgatgatctg ccggcatctc ctgtctcctg ttccctgtcc
      301 cctgtctccc gatcggacgc gacgtggcag ccaggcgacc ccgtcgctct ccttctgcac
      361 ggcctcggcg gctgtcatca gtcgggctac atgcagcgct gcgccgtgaa gctcgccgag
      421 cgcggctatc gcgtgtttcg catggacctc cgcggttacg gcgcggggtt tccgctggcc
      481 cgccatccgg tgcatgccgg ccgcagcgag gacgcggccg ccgcgctgag ctacgtgatc
      541 gagacttgcc ccgattcgcc gattcacctg gtcggcttct cgatgggggc caacatcgtg
      601 ctcaagctgg ccggcgagct ggggagccaa gcgcccccgc acctggccag cgtcatggcc
      661 atcggcccgc cgatcgacct catcgagtgc tccaaaaaca tgcagcgggg ctggaatcgg
      721 ctgtacgacc ggcggttcgc gcggaacctg gtgcgcttca tccagcagcg gtcgcaggtc
      781 cggcccgacg ccctctcgcg gccgctggtg ccgcagcctc gcagtctgct cgatttcgac
      841 gcgattttca ccgcgccgct atcgggcttc gccgacacgg acgactacta tacgcaggcc
      901 agcagcggac gcctgctgcc gcggatcacc gtgccgacct tgatcgtcac ggcggccagc
      961 gatccgatca ttcccgtcgg gccattcgag cgggccagct attcacccag tacgcagctc
     1021 atcattactc cctgcggcgg ccacctgggc ttcgtgggcg cccgcggcgt tgaccccgac
     1081 caccgctggc tcgactggcg gatcgtggag tgggtcgcct cccacgcggt ccggcccgcg
     1141 gccgatccgc ggtctcgacc gaccgcccat tcgcgggacg cgaccccggc attgtgaaca
     1201 ttggtacgct atttgcagtg tagttccgat tgcgcggcgg tcggccattt tggccgcccg
     1261 tcgccctgct ctgtctcaat ttggtcgttc cacgctaggg ccgaatcggc accctaattg
     1321 aacttctgtt tatataacga aaactcgtca cccatctctc taaggcttca aaccctcatg
     1381 cgatccaccg ccggcggctt cgccggaact gcttgaaaac aagggggttg cggcgcgact
     1441 gctggcgcga tggggcgaga aaaaatcctc gcccgcggca gttttcgtta tattcgccgg
     1501 cggttgctta gggcggtcta tcgcccgaac agctccgtcc agtgctggtt gtgccggccc
     1561 agcccgacgc gggtgtggct gccgagcatg ttgcggtggt ggccggggct gtgccaccac
     1621 atttgattga ctacggcgcc gtcgagcgtg cccagggcga tgttctcgcc cgaagccgtc
     1681 gtgccgaact tctcggcccg gtcccaggga gtcttcttgc cggggacagg cgattcgtgg
     1741 tcgaaaaact tctgctcctc catgtcgcgc gagtggtcgc gggcggcagc cgccagcttc
     1801 aggtcgatca ccagcgggtc gaggccgagc aggttgcggg tcaggttcag gtcgaggacg
     1861 cagcgggcct cttcggggtc gagctggcca gcgagctgat tgttctgggc cagcgtccgc
     1921 cgggcggcgt cgctcatcgg cgtagccagc gccgtggcga tttcctcgtc cttgaccagg
     1981 tacttttcga acgagggcgt cgtgtccagt tcagcgatct cctcggcagg ctgtttttcc
     2041 tgcggcgcgg cgtcggtttt cgccggttca ggtttcgcgg cgaggatcag cgtcgcgcac
     2101 aattcccact ccttgcccag cacttgcagc cggttgcggg ccgcgatgag gttggcattg
     2161 gaatccagga tgtccttgcg gctgacgaga atcagctcct tcaagcgagc caggcccgga
     2221 tcgctggtgg ccacgatctc ggccttggtg aggtcgtcct tcttggccag ggccagcaca
     2281 ttctcgcgga tctgggtgat ctcctcgagc ttgtccgccg ctgtccggcc ggcgatcacg
     2341 gcggccgcag cccgcaggaa ctgctgccgg tacgccgcca gttgcggatc gagctcct
//
LOCUS       DASRJB010000599         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1585209, whole genome shotgun sequence.
ACCESSION   DASRJB010000599 DASRJB010000000
VERSION     DASRJB010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585209"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            619..>2416
                     /locus_tag="VFB80_15795"
     CDS             619..>2416
                     /locus_tag="VFB80_15795"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN35292.1"
                     /translation="MLLRPLLLLLIGTCAAAAEPQFTAEQLEFFTREVKPILQANCVV
                     CHGGEKKIQSNFNLTSRGGLLKGGEIGGPMSLEKPEASLLLEAINWDSLEMPPKGKLP
                     QAQIDVLTKWVKWKLPWPDGESGVLVHRGPPRVDEAARNFWSYRPVVRPPVPAVKDAG
                     WVKTPLDAFVLAKLEEKGLRPAPPADKATLLRRAYYAVTGLPPSSDAVTAFLADSSPT
                     AYENVVEDLLESRHYGEHWARHWLDLVRYAESNSFERDNPKPYVWRYRDYIIRSLNAD
                     KPYDQFIREQLAGDELDQVTADTIIATGYYRLGLWDDEPADPLLAYYDGLDDILATTG
                     QTFLGLTVNCARCHDHKLDPFPQRDYYRLLAFFHGVKHYGDRSDQSVAAASLRTIGGA
                     EDQERQRELIAQHERKITENEGQQREIEALIADKLPGGERDDFKSPGNRVRLMRKHIG
                     ELITQEQFDNYARLKFARSRLDRERPQASAQALAVKEDSQPRETFVLLRGNPQGKGDR
                     VEPGFPSVLLAEGSSPPQIPAPPDGSNSSGRRRVLADWVASSNNPLTSRVIVNRVFQY
                     HFGRGIVRSSSNFGYQGTPPTHPELLDWLASEF"
BASE COUNT          434 a          795 c          756 g          431 t
ORIGIN      
        1 cgcggtcttc gtcatggcaa gtctcctgag taggttccgc ctgccgggcg gaacctgatc
       61 gcgggggaac gtctttgctg gccgcagagg tctcgcccgg caggcgagac ctacctacat
      121 gcgcgttctg tacacgtatg ctcaggttct gctacttgcg tgaacacccg tcaacaaccc
      181 cccgcccaga attctcaccc ccctgcctgc acgcctcgcc gcaacccctt gccctcacgc
      241 cctttacaac cacaaaaaaa ccgccaaatt cccaccaacg cgttttcaga atggactagt
      301 gtggtccatt ccgcgcctcc ctatttcggg gtgttttaag aaattcctga tgcctgaagc
      361 gccagcatcg ctacctagcg caccaggaac gcagggcgcg gcctcttggt gcctggtgat
      421 ttgcgagtcc ttggtattaa ccttacggcg cttccttcgc tggcgcttca ggcttcaaat
      481 ctcatgggcc ggaccagaag cttcaccgtt tggagcaatc gcatgtcggc catttcgcac
      541 cttccggctg cgcacggggc ggcctaaaat tcaggcttcc cgccgtggtc tggccttcct
      601 gctgctcgga gcacgtgaat gctgttgcgt cccttgttgc tgttgctgat cggcacttgt
      661 gcggcggcgg cagagccgca attcaccgcc gagcagttgg agttcttcac gcgcgaagtg
      721 aagccgatcc tccaggcgaa ctgcgtcgtc tgccacggcg gcgagaagaa gattcagagc
      781 aacttcaacc tgaccagccg cggcggtctg ctcaaggggg gcgaaatcgg cggtccgatg
      841 tcgctcgaga agcccgaagc gagcctgctc ttggaggcga tcaactggga cagcctcgaa
      901 atgccgccca agggcaagct gccccaggcg cagatcgatg tgctcacgaa atgggtgaag
      961 tggaagctgc cctggcccga cggcgagagc ggagtgctgg tgcatcgcgg gccgccgcgc
     1021 gtcgacgagg cggcgcggaa cttctggtcg tataggccgg tagtgcgtcc gcccgtgccg
     1081 gcggtgaagg acgcgggctg ggtgaagacc ccgctcgatg cgttcgttct ggcgaagctc
     1141 gaggaaaaag ggctgcggcc cgcgccgccg gccgacaagg cgacgctctt gcggcgggcc
     1201 tattacgccg tgacgggcct gccgccttcg tccgacgccg tgactgcgtt tctggccgac
     1261 agttcgccga cggcctacga gaacgtcgtc gaggacttgc tggagtcgcg gcattatggc
     1321 gagcactggg cccggcactg gctcgacctg gtgcgctacg ccgagtcgaa tagttttgag
     1381 cgggacaatc ccaagccgta tgtctggcgg tatcgcgact acatcatccg ctcgctcaac
     1441 gccgacaagc cctacgatca gttcatccgc gagcagctgg cgggagacga gctggaccag
     1501 gtcaccgccg atacgatcat cgccacgggc tattaccgcc tgggtctgtg ggacgacgag
     1561 ccggccgacc cgctgctggc ctattacgac ggtctcgacg acatcctcgc tacgacgggc
     1621 cagacgttcc tcgggctcac ggtcaactgc gcccgttgtc acgaccacaa gctcgatcct
     1681 ttcccgcagc gcgattatta ccgcctgctg gcgttctttc acggcgttaa gcactacggc
     1741 gaccgcagcg atcagtcggt cgccgcggcg tcgctgcgga cgatcggcgg ggcggaggac
     1801 caggagcggc agcgcgagct gattgcgcag cacgagcgga aaatcaccga gaacgaaggg
     1861 cagcagcgcg aaatcgaggc cctcatcgcc gacaaactgc ccggcggcga acgcgacgac
     1921 ttcaagtcgc ccggcaaccg cgtgcggctg atgcgcaaac acatcggcga gctgatcacg
     1981 caggagcaat tcgacaacta tgccaggctc aagttcgccc gatcgcggct cgaccgcgag
     2041 cgcccgcagg cctctgccca ggcgctggcg gtgaaggaag attcgcagcc gcgagagacg
     2101 ttcgtcctgc ttcgcggcaa tccgcagggc aaaggggacc gtgtggagcc cggatttccg
     2161 agcgtgcttc tggctgaggg cagctcgccg ccgcaaattc ccgcgccgcc ggacggcagc
     2221 aattcctccg gccgccgccg cgtgctggcc gactgggtcg ccagctccaa caatccgctg
     2281 accagccgcg tgatcgtcaa ccgcgtgttt cagtaccact tcggccgcgg gatcgtgcgg
     2341 tcgagcagca attttggcta tcaaggcacc ccgcccacgc acccggagct gctcgactgg
     2401 ctggccagcg agttcg
//
LOCUS       DASRJB010000600         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1862032, whole genome shotgun sequence.
ACCESSION   DASRJB010000600 DASRJB010000000
VERSION     DASRJB010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862032"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFB80_15800"
     CDS             <1..535
                     /locus_tag="VFB80_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35293.1"
                     /translation="GEFVKNLPPGFQWFGLGQDLGQWCLVGIALAVFATLAWSLRNLA
                     AGRAVYATGSDPEAAFLAGLAPRRVTFGVFAALGALTGLAALLNGVRFADCDPSAGNG
                     LEMQVIAAVVVGGVAISGGRGTLVGPLLGVLLLATIGPALVFLGSQPYWEKTIQGAII
                     LVAVATDSLARGRRSHV"
     gene            528..>2213
                     /locus_tag="VFB80_15805"
     CDS             528..>2213
                     /locus_tag="VFB80_15805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN35294.1"
                     /translation="MSSRTGRLPQEAILAALLAAEVALFAAIGKNFFTAANAFEIVRL
                     SVEVGLLALALTPVIVSGGIDLSVGSLIGLSAVLFGMLWRDAHLPIPLAALVTLAAGA
                     LAGTLNGLLVTLLRLPPLIVTLGTFSLFRGLAEGLTRGVENYTSFPASFLFFGQGYLP
                     LGVPAQLPLLIVVAAAYWWLLHLTATGRGLVAIGFSAEGARHAGIPVERRLLIVYVLS
                     GLVASLAAVLYVAHLGQAKADAGTGYELMAITAVVLGGTSIFGGRGTIHGTLLGLVAI
                     VVLQNGLRLAALPAELAGILIGGVLLGTIALERLSALPRAAARATMEEFEVKNSQVAV
                     ICGVILVAAGIIAGSNWYALREQARQLELSSSRLMQLAPAASPQHTPPIARTITVAMM
                     PKSKGNAYFIACRQGAEEAAKELGIELIWDGPTDPDPAKQNEVIEAWITRGVDVIAVA
                     VENRSGVSSVLRKARQRGIKVLTWDADAEPDARDFLVNQATPQGIGTTLMDTAAKVMG
                     GKGEFAIITASLTAANMNEWQKAIEARRAEKYPDIRMVALQPCDDLQKKAFDAA"
BASE COUNT          314 a          727 c          786 g          386 t
ORIGIN      
        1 aggcgagttc gtgaagaacc tgccgcccgg gttccagtgg ttcggcctgg gccaggatct
       61 tgggcagtgg tgcctcgtgg gcatcgcgct ggcggtcttt gcaacgctcg cctggagcct
      121 gcggaacctc gccgccggcc gcgcggttta tgccacgggc tccgatcccg aggcggcgtt
      181 cctggcggga ctcgcgccac ggcgggtgac gttcggagtc ttcgcggcgc tcggcgcgct
      241 caccggcctg gcggcgctgc tcaacggcgt gcggtttgcc gactgcgacc ccagcgcggg
      301 caacggactt gagatgcagg tgatcgcggc ggtcgtcgtg ggcggcgtgg ccatctccgg
      361 cggccggggc acactcgtcg ggccgctgct gggcgtcttg ctgctggcga cgatcggccc
      421 ggcgctcgtg ttcctgggct cgcagccgta ttgggaaaag accattcaag gcgcgatcat
      481 cctcgtggcg gtcgccaccg acagcctggc ccgcgggcgg aggagccatg tctagccgca
      541 cgggccgcct gcctcaggaa gcgatcctcg ccgcgctgct cgcggccgag gtcgcgctgt
      601 ttgccgcaat cggcaagaac ttctttaccg cggccaacgc cttcgaaatc gtgcggctga
      661 gcgtcgaagt cggcctgctc gcgctggcgc tgacgccggt catcgtgtcc ggcggcatcg
      721 acctgtcggt gggctcgctg atcgggctgt cagctgtcct tttcggcatg ctgtggcgcg
      781 acgcgcacct gccgattccg ctcgccgcgc tggtcacgct ggcggcgggc gctctggcgg
      841 gaacgctcaa cggcctgttg gtcacgttgc tgcgcctgcc gccgctgatc gttacgctcg
      901 gcacgttttc gctgtttcgt ggactggccg aggggctgac gcgcggcgtc gagaactaca
      961 ccagctttcc tgccagcttc ctgttctttg ggcagggcta tctgccgctg ggcgtgccgg
     1021 cgcagctgcc gctgctgatc gttgtcgccg ccgcttactg gtggctgctg cacctgactg
     1081 ccaccggtcg cgggctggtg gcgattggat tttctgccga gggtgctcgc cacgcgggca
     1141 tccccgtcga acgccggctg ctcatcgttt atgtgctctc gggcctcgtc gccagcctgg
     1201 cggccgtgct gtacgtcgcg caccttggcc aggcgaaagc cgacgcgggc acgggctatg
     1261 aattgatggc gatcaccgcg gtcgtcctcg gcggcacttc gatcttcggc ggccgtggca
     1321 cgatccacgg cacactgctg ggcctggtgg cgatcgtcgt gctgcaaaac ggcctgcggc
     1381 tggcggcact gcccgccgaa ttggccggca tcttgatcgg cggcgtattg ctcgggacga
     1441 ttgcactgga gcggctgtcg gcgctgcccc gcgccgcggc ccgggcaacg atggaggaat
     1501 tcgaagtgaa gaactcgcaa gtcgcggtga tctgcggcgt gatcctggtg gcggcgggca
     1561 tcatcgccgg cagcaactgg tatgccctgc gcgaacaggc acggcagctg gagctgagct
     1621 cctcgcgctt gatgcagctc gctccggccg cgtcgcccca acacacgccg ccgatcgcgc
     1681 ggaccatcac cgtcgccatg atgcccaaaa gcaagggcaa cgcctacttc atcgcctgcc
     1741 ggcaaggcgc ggaagaagcg gccaaagagc tcggcatcga gctgatctgg gacgggccga
     1801 ccgatcccga tccggcgaag cagaacgaag tgatcgaggc ctggatcacg cggggcgtcg
     1861 atgtgatcgc ggtcgcagtc gagaaccggt cgggcgtctc gtcggtgctg cggaaggctc
     1921 gccagcgggg catcaaggtc ctgacgtggg acgccgacgc cgagccggac gcccgtgact
     1981 ttctggtcaa tcaggccacg ccgcagggca tcggcaccac gctcatggac acggccgcga
     2041 aggtgatggg cggcaaaggc gagttcgcca tcattaccgc gtcgctgacg gccgccaaca
     2101 tgaacgagtg gcagaaggcc atcgaggccc ggcgggcgga gaagtacccg gacatcagga
     2161 tggtcgccct gcagccgtgc gacgacctgc aaaagaaagc gttcgacgcg gcg
//
LOCUS       DASRJB010000601         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_173709, whole genome shotgun sequence.
ACCESSION   DASRJB010000601 DASRJB010000000
VERSION     DASRJB010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173709"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VFB80_15810"
     CDS             complement(<1..955)
                     /locus_tag="VFB80_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZN35295.1"
                     /translation="MSDLHHECGIAAVYHLPGEPHPLCPDGGPDEISRLVPRMLLDIQ
                     NRGQLAAGMTSFNPHRDQLLDTHKDVGTVSEVFRLSHRGKAESLMRQYAGRAAIGHVR
                     YATCGSEDRNYAQPFERHHLEKRKWFAFGFNGQLANFQELKEKLLSDADHHLARENDT
                     EIMMHEISRELSGDRRPRLVEVMRSVSQRFDGAYSIVFLNAQGDLLVARDPLGIKPLC
                     YAKEGNLFAAASESVPLLNLGFAPENIHSLAPGHAISITGGKFELEPFATPQRTAHCY
                     FEWIYFANVASTLDERSVYLSRTALGEELARLEMADGRVPLD"
     gene            complement(1064..2326)
                     /locus_tag="VFB80_15815"
     CDS             complement(1064..2326)
                     /locus_tag="VFB80_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN35296.1"
                     /translation="MKRYLGWAAWQASALAAALFIAGALRAGDWPQILGPTRSGHAES
                     ERLLATWPAGGPKLLWKFPLGSGYAGAAVMSGKVVVFHLSGASERVECLDATSGRSLW
                     KADFEANYRGGIDADKGPRCVPLVAGNAVYAFGAAGDLHAVALDSGKALWSRALCDDY
                     KSDLGYFGAGSTPVLAGGRLLVNVGGKGAGLVALDPATGKTLWQATDEVASYSAPAAI
                     TLGGKEQALFITRMNCVLADPATGNVKSLFPFGARGPTVNAATPLVFGGKLFTTASYG
                     IGAVLASLDGAGARTIWSNDDTLSSQYATPVEHEGFLYGTHGREDQGVAELRCVEVAT
                     GKVRWTQPNFGVAHAILADGKLLLQTADGRLALAAADAAKYRELASAQVAQEPTRALP
                     ALAAGQLYVRTGSGGGQLLCFRVGNAAE"
     gene            2559..3671
                     /locus_tag="VFB80_15820"
     CDS             2559..3671
                     /locus_tag="VFB80_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35297.1"
                     /translation="MARLFRFVEKKRGGRRTGSNLVGNFGEALFFGSLFLVGMLALSV
                     LLASHLLAPDPASFTIGFGKWLFLLLMASFVLLGGGGMIWTVLRIGVSAERRSALATR
                     AVDLDLMQEAVPRPRNYPAIPPFDGLVNSPGIDLAYRLPPAQTPGWQLLAMTIFGLLW
                     TGVVCFFTVWTISNALAGRPEWLLTALLVPFWAVCYWAIREFLQLLVVNSGTGSTTVE
                     ISDLPLAPGHEYQVAIAQHGQLTMKSLELWLVCEEEATFTQGTDIRSEVREVWRQLCF
                     ERRDFRIEAGVPFQEQCAIAVPPTAMHSFYSPHNAVRWKLVARGEADGWPAFERGFPL
                     VVYPGEATQQVEVAGHVARAALTPPHVPLPAAGVRA"
     gene            3668..4078
                     /locus_tag="VFB80_15825"
     CDS             3668..4078
                     /locus_tag="VFB80_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35298.1"
                     /translation="MIEPLLSLCLAGPNRNFAAGDELVCEYQIDAVEPADIQAVELSV
                     LWYTEGKGEEDMGVHFFERRLPADAEDGDLRPMRRLRSKLPNSPLSYRGAIVSVFWCV
                     RLRLFLRRGREFVVEEPFTLGSVPAVLPAIAEPS"
     gene            4119..4697
                     /locus_tag="VFB80_15830"
     CDS             4119..4697
                     /locus_tag="VFB80_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35299.1"
                     /translation="MTSNPFATRHIRPGAVDYIFPPGQSAAALIERLRASGWFGQIIG
                     PHGSGKSTLLAALAPALKQAGRTVHSYHLGTLRQFHPKDYLDKLPPSGRGLLVIDGYE
                     QLSWWWRRRTLATCRRHDIGLLVTAHQDMGLPTLLTTEPTLALAKQVVRRLLGPDEAT
                     IAGGDVKRAWDEAGGNLRETLFKLYDVYQARR"
     gene            complement(4699..>6100)
                     /locus_tag="VFB80_15835"
     CDS             complement(4699..>6100)
                     /locus_tag="VFB80_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN35300.1"
                     /translation="VLKAFDEKLQRVVALKVMSPRLAGNAASRKRFIREGRAAAAVCH
                     EHVVTIHAVEEGPLLPYFVMQYISGHSLQQRLERSGPLEVTEILRIGMQAAEGLGAAH
                     AQGLVHRDIKPANILLENGVERVKITDFGLARAVDDASLTKSGVIAGTPLFMSPEQAR
                     GEAVDHRADLFSLGCVLYTLATGRPPFRAATTMAVLRRICDDEPRPIRELNPEIPDWL
                     AAIITRLLAKDPLQRYSSAKELAELLAGCLAHAQQPGSVQLPVVIGNIPPPVSKATSL
                     PAPAEGQRRSWFATPAGIAVLVGVVIAPLVLLLGCAGLLVLGYWIMPIGGTPLQAPLA
                     PLEPRIVGDRSRVTASAAELTELRRLVDIAEEEMRQSKALYDGGRIAAGEHLQTEINH
                     CTAQAGYFHASGQLAERCDALRRKVKLHEQLVHFADERVKANVGVPAERLAAEGALTK
                     AKLELRAAEGEMAGRP"
BASE COUNT          954 a         2102 c         2009 g         1035 t
ORIGIN      
        1 tgtcgagcgg gacgcggcca tcggccatct ccagccgggc cagctcctcg cccagcgccg
       61 tccgcgacag gtagacgctc cgctcgtcga gcgtgctcgc gacgttggcg aaataaatcc
      121 actcgaagta gcagtgcgcc gtccgctgcg gcgtcgcgaa gggctccagc tcgaacttgc
      181 cgccggtgat cgaaatcgcg tgccccggcg cgagcgagtg gatgttctcc ggggcaaagc
      241 ccaggttgag gagcggcacg ctctcgctgg ccgcggcgaa caggttgccc tctttcgcat
      301 agcacagcgg cttgatgccc agcggatcgc gggccacgag caagtcgcct tgagcattca
      361 gaaagacgat gctgtacgcc ccgtcgaacc gctggctgac cgaacgcatc acctccacca
      421 gccgcgggcg acggtcgccc gataattcgc ggctgatctc gtgcatcatg atctcggtgt
      481 cgttctcgcg ggccaggtgg tggtcggcgt cgctcagcag cttctccttc aactcctgga
      541 aattcgccag ctgcccgttg aacccgaacg cgaaccactt gcgtttttcc aaatggtgcc
      601 gctcgaacgg ctgagcgtag ttgcggtcct cggagccgca ggtcgcatag cggacgtggc
      661 cgatcgcggc ccggccggcg tattgccgca tcaggctctc ggccttgccg cggtgcgaga
      721 ggcgaaagac ctcgctgacc gtgccgacgt ctttgtgcgt gtcgagcagc tgatcgcggt
      781 gcgggttgaa gctcgtcatc cccgcggcga gctgcccgcg gttctgaatg tcgagcagca
      841 tccgcggcac gagccgcgag atttcgtccg gcccgccgtc gggacagagc ggatgcggct
      901 cgccgggcag gtgataaacc gcggcgatgc cgcattcgtg gtgcaggtcg ctcatcagca
      961 gtgccaactg gggtaggcgt gggcggcccg cagggccaag gtcgaggcga agcggttact
     1021 tatgaatttt agttttcgag ggcaaaatcg gcaatgggca gtttcactcg gctgcattac
     1081 cgacgcggaa gcagagcagc tggccaccgc cgctgccggt gcgaacgtag agctgccccg
     1141 cggccagggc gggcaaggcc cgcgtcggct cttgcgccac ttgggccgag gccagctcgc
     1201 ggtacttcgc cgcatcggca gcggccagcg ccaagcgtcc gtcggccgtc tgcaacagca
     1261 gcttgccgtc ggccaggatc gcgtgggcca cgccaaagtt cggctgcgtc caacggacct
     1321 tgcccgtcgc cacttcgacg caccgcagct cggcgacgcc ctggtcctcg cggccgtgag
     1381 tgccatagag aaacccctcg tgctcgaccg gagtcgcgta ttgactggag agcgtgtcgt
     1441 cgttggacca gatggttctg gctcccgcgc cgtcgagcga tgccaggacg gcgccgatgc
     1501 cgtaactggc agtcgtgaac agcttgccgc cgaagacgag cggcgtggcc gcgttgaccg
     1561 tcggcccgcg ggccccgaag ggaaacagtg atttgacgtt gccggtcgcc ggatcggcga
     1621 gcacgcaatt catccgcgtg atgaacagcg cctgctcctt gccgcccagc gtgatggcgg
     1681 ccggcgcgga atagctggcg acctcatcgg tggcttgcca cagcgttttt cccgtggccg
     1741 gatcgagagc gacgaggcca gcgcccttgc cgccaacatt caccagcagc ctgccgccgg
     1801 ccagcacggg cgtgctgccc gcgccgaaat agcccaggtc gctcttgtag tcgtcgcaca
     1861 gggcccgcga ccagagcgcc ttgccggaat cgagggcgac cgcgtgcaga tcgccggccg
     1921 cgccaaaggc atacaccgca ttgccggcaa ccagcggaac gcaccgcgga cctttgtcgg
     1981 cgtcgatgcc accgcgatag ttggcttcga agtcagcttt ccacagcgag cggccgcttg
     2041 tcgcgtcgag gcactcgacc cgctcgctcg cgccgctaag gtgaaagacg accactttac
     2101 cggacatcac cgccgcaccg gcatagccgc tgccgagcgg gaacttccag agcagcttcg
     2161 gcccgccggc tggccaggtg gccagcagcc gctcgctctc ggcgtgtccg ctgcgcgtgg
     2221 gcccgagaat ttgcggccaa tccccggccc gcaacgcgcc ggcaatgaac agcgctgccg
     2281 ccagggccga ggcctgccag gctgcccaac cgaggtaacg tttcatatca gcttccttgg
     2341 atatttgcga cattttactg gctgcgagct gcttccaagc cagaactcgc cccacaaagg
     2401 ccaatctgcg gcgattgtag tccctttggc cgcgagggga cacaaaccac ccggaacccg
     2461 accttgcggc ctgggtcgag tttgtcgaga acggggaaat tctctaagat attttcagga
     2521 acagcccgca aagggattaa gggaccggag cggcattagt ggctcgactc tttcgcttcg
     2581 ttgagaagaa gcgcggcggt cgccgcacgg gatcgaatct cgtcggcaac ttcggcgagg
     2641 cgctcttctt cggctcgctg ttcctggtcg gcatgctcgc gctgtcggtt ttgctggcca
     2701 gccatctgct cgctcccgat ccggcgtcgt tcacgatcgg cttcggcaag tggctgttcc
     2761 tgctgctgat ggcgtcgttc gtgttgctcg gcggcggcgg aatgatctgg acggtgctgc
     2821 ggatcggcgt ctcggccgag cggcgcagcg ccctggccac gcgggcggtc gatctcgatc
     2881 tgatgcagga ggccgtgccg cggccgcgga actatccggc cattccaccc ttcgacgggc
     2941 tggtcaacag ccctggcatc gacctggcct atcgcctgcc gccggctcag acgcccggct
     3001 ggcagctgct ggcgatgacg atcttcggct tgctgtggac cggcgtcgtc tgctttttca
     3061 ccgtctggac gatcagcaac gcgctggccg gacggccgga atggctgctg acggcgctgc
     3121 tcgtgccgtt ctgggcggtt tgctactggg cgatccgcga gttcctgcag ctgctcgtgg
     3181 tgaatagcgg cacgggatcg acgacggtcg agatctcgga cctgccgctc gcgccgggcc
     3241 acgaatacca ggtggcgatt gcccagcacg ggcagttgac gatgaagtcg ttggaactgt
     3301 ggctggtctg cgaggaggag gccaccttca cgcaagggac cgacatccgc agcgaagtcc
     3361 gcgaggtctg gcggcagctg tgcttcgagc ggcgcgattt ccgcatcgag gccggcgtgc
     3421 cgttccagga gcaatgcgcg atcgcagtgc cgcccacggc gatgcattcc ttctattcgc
     3481 cgcacaacgc cgtgcgctgg aagctcgtgg cccgcggcga ggccgacggc tggccggcgt
     3541 tcgagcgcgg gtttccgctc gtcgtctatc cgggcgaagc gacgcagcag gtcgaagtgg
     3601 ccggccacgt ggcccgcgcg gccctcacgc cgccgcacgt gcctctgccc gcggcaggag
     3661 tccgggcgtg atcgagccgc tgctcagcct gtgcctcgcc ggcccgaatc ggaacttcgc
     3721 ggccggcgac gagctcgtct gcgaatacca gatcgacgcg gtcgagccgg ccgacatcca
     3781 ggccgtggaa ctttccgtgc tgtggtacac cgagggcaag ggggaagaag acatgggcgt
     3841 gcacttcttc gaacgccgcc tgcccgccga cgccgaggac ggcgacctgc ggcccatgcg
     3901 gcggctccgc agcaagctcc ccaacagccc cctcagctac cgcggggcga tcgtcagcgt
     3961 cttctggtgc gtgcggctgc ggctgtttct tcgccgcggc cgggaattcg tcgtcgaaga
     4021 gcccttcacg ctgggcagcg ttccggccgt cttgccggcg attgccgagc cgtcgtaacg
     4081 tggtgcaggc ccactcggct ggcccgcgga ctgaccacat gacctccaac ccatttgcca
     4141 cgcgccacat ccgtcccggc gcggttgact atatcttccc gcccggccag tccgcggcgg
     4201 cgctgatcga gcggcttcgc gccagcggct ggttcggcca gatcatcggc ccgcacggca
     4261 gcggcaaatc gacgctgctg gcggccctcg cgccggcgct gaaacaagcc ggccgcacgg
     4321 tccacagcta ccacctcggc acgctccggc agtttcaccc gaaggattat ttggacaagc
     4381 ttccgccgag cgggcgcgga ctactcgtca tcgacggcta cgagcagctt tcctggtggt
     4441 ggcggcggcg aacgctggcc acttgccgac gccacgacat cgggctgctc gtcaccgcgc
     4501 atcaggacat gggcctgccg acgctgctga cgactgagcc aaccctagcg ctggccaagc
     4561 aagtggttcg ccgcttgctc ggcccggacg aagccaccat cgctggcggc gacgtgaagc
     4621 gcgcctggga cgaagccggc ggcaacctgc gcgagacgct gttcaagctg tacgacgtgt
     4681 accaggcccg ccgctgactc aggggcgacc ggccatttcc ccctcagccg cccgcaactc
     4741 gagcttcgcc ttcgtgagcg ccccttcggc ggccagccgc tcggcgggga cccccacatt
     4801 cgccttcaca cgttcgtcgg cgaaatgcac caactgttcg tgcaacttca ccttcctccg
     4861 cagtgcgtcg cagcgctcgg ccagctgacc ggacgcatgg aaatagccgg cctgcgcggt
     4921 gcagtgattg atttccgttt gcagatgctc gccggcagca atcctcccgc cgtcgtacag
     4981 cgctttcgat tgccgcatct cctcctcggc gatgtccacc agccgtctca gctcggtcag
     5041 ctcggccgcc gatgcggtca cgcgactcct gtcgccgacg attcgcggct cgagcggcgc
     5101 gagcggcgcc tggagaggcg ttccgccgat cggcatgatc cagtagccca gcaccagcag
     5161 gcccgcacaa ccgagcagca ggaccagcgg cgcaatcacc acgccaacca gcacggcgat
     5221 tcccgccggc gtcgcgaacc agctgcggcg ctggccttca gccggtgcag gcagcgacgt
     5281 cgccttggaa accggcggtg gaatattgcc aatcaccacc ggtagttgca cgctgcccgg
     5341 ctgctgcgcg tgggccaggc agccggcgag caactcggcc agttccttcg cggacgagta
     5401 gcgctggagc gggtccttgg ccagcagccg tgtgataatt gccgccagcc agtcgggaat
     5461 ctcggggttc agctcgcgga tcggccgcgg ctcgtcgtcg caaatccgcc gcagcacggc
     5521 catcgtcgtc gccgcgcgaa aggggggccg gcctgtcgcc agcgtgtaga gcacgcagcc
     5581 caggctgaac aggtccgccc ggtgatcgac cgcctcgccg cgggcctgct cgggcgacat
     5641 gaacagcggc gtccccgcga tgacgccgct cttggtcagg ctggcgtcgt ccaccgcgcg
     5701 ggccagcccg aagtcggtga tctttacccg ctcgacgccg ttctccagca agatgttcgc
     5761 cggcttgatg tcgcggtgca ccaggccctg ggcgtgggcc gcccccagcc cctcggccgc
     5821 ctgcatgccg atccgcagga tttccgtcac ttccagcggg ccgctgcgct ccagccgctg
     5881 ctgcagcgaa tggccgctga tgtactgcat cacgaagtac ggcagcagcg ggccctcttc
     5941 gaccgcgtgg atcgtcacga cgtgctcgtg gcagacggct gcagccgccc ggccctcgcg
     6001 gatgaaccgc ttgcggctgg cggcattccc ggcgagccgc ggcgacatca ccttcagcgc
     6061 gaccacgcgt tgcagctttt cgtcgaacgc tttgagcacg
//
LOCUS       DASRJB010000602         5803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2996042, whole genome shotgun sequence.
ACCESSION   DASRJB010000602 DASRJB010000000
VERSION     DASRJB010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996042"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFB80_15840"
     CDS             complement(<1..674)
                     /locus_tag="VFB80_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35301.1"
                     /translation="MRHTLLFLALAPVLFCSCKGVGQSFLIAKKDGAPAEPTRQVSRA
                     APRYPVQQAQYSLPVHDTLPPSAWTGGVARDSVAGIPPWNGGLHADGGYCPHCGPAGL
                     PPFHFSDEGPSEELSWMPDGLKCPWPQDEFICDGGDLNHDVHVKKDWTVVGLDQEDTV
                     AHFDTLDGRTEIVESNCVCIYAPRFAAVRQVSSPILHEGHERMADVVMPVKPNLHEEN
                     RIPKTAL"
     gene            complement(697..1458)
                     /locus_tag="VFB80_15845"
     CDS             complement(697..1458)
                     /locus_tag="VFB80_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35302.1"
                     /translation="MLRAVANLRQVAAVAAWLAASAMGLPVASAQGPWHYLNKADMPP
                     GAIGLRQLQRGGPLPGYFQPVEVTAPAATLLSVVSEGEFSEAKREKLLAGMLIGQVYR
                     LKVSNIPGREGTEIFPTIEVIDRLYPPPGQAARFPIPVELTEEEIKMAVDGRYVLRVI
                     YVEEPRTALPLRDQPGRQRYFEIRPGDDAMETADRLGRPVAILRMGSRVPLPDEDQSH
                     FLYHQPPVQLIEELPPALPRNQGLEERLPAPPRLP"
     gene            complement(1587..2744)
                     /locus_tag="VFB80_15850"
     CDS             complement(1587..2744)
                     /locus_tag="VFB80_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35303.1"
                     /translation="MKVRLCLVSASLAVCLTALGCAFPKQHNLPPAPRLLEPGPGVGG
                     PGPGVLPPSLPPAMMPVAMPSVQVNFARPEAMQIRWDVGGVGQFDSTPLIVPGRQNFP
                     QNGLYRLKLTDIPGRAGLELYPTVEIGPTTPRTEAFLAHNAIPVQFTEEDFDQVQAGN
                     FVTKVIYLPDPEFQELALAGVDTLVSTRLDPGVDPIVEADRRGSIMAVVRLGNKDVEL
                     AGAEEGLGGFEGGGFGGGFGGPCPMPFGASGLVPNYVAGITAPQYGMPYVGTPIGLPG
                     PPHVPFGGPAGLQKHVMKNHTHMSIPGPTQNVKIHVRQQPGQSYPKPANRAWINEQMI
                     HPSLPFEQPHWDMHEGLRGGVPAPVQGGAIHGRHGAGGMPAGYCPPEEMGH"
     gene            3045..3968
                     /locus_tag="VFB80_15855"
     CDS             3045..3968
                     /locus_tag="VFB80_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35304.1"
                     /translation="MVSALCLGSWLLAPGPWPLFQEPVASGQEPAAGGQQLLAEAVRR
                     LDGQTAISAELRYRVNAFGHDLAGTGSYLQLGAGPQRLLRLELRMQVGEQPATLLEIR
                     GTDRYWIRRDVPPNPPTLGRVNLVQLRRGLRRPDQPGGDDALPRGAWIILGGLPRLLE
                     ALENHFDFAAPRADEVFVAGSGGERQALPIWVLDGKWKPDRLAALSGRQSPAEGSLPE
                     QLPDRVEIVLDRSDQSLPLFPYRVSYWKSAPQTAGNAAAQRELLSLELFNVHRSGQID
                     SRQFEYHAGDQEVLDLTAAYIQRLGGPARQR"
     gene            4059..5063
                     /locus_tag="VFB80_15860"
     CDS             4059..5063
                     /locus_tag="VFB80_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35305.1"
                     /translation="MKLTFSLALALLTISLSVQAADPPLKVGIIGLDTSHVIAFTKTM
                     NTPGPKADAINCRVVAAYPKGSPDIESSTSRVPGYIEEMKKHNVEIVDSIEELLKRVD
                     CVLLETNDGRPHLEQALPCLKAGKPTFIDKPLAGSLADCLAIVDAAKKYNCPMFSSSS
                     LRYGKLTQEVRGGSLGKITSAETTGSAALEKTHPDLFWYGIHGVESLFAVMGTGCELV
                     TRKTTADGKIEVVGEWVGGRKGTFRESQKYGGLAKGDKGEGPVGDSAGYDPLVLEIGK
                     FFHSKKPPIPQAETLEIYAFMEAADESKRRGGEPVEIEEVIAKAQPLAAKRLAELGVK
                     "
BASE COUNT          978 a         1889 c         1929 g         1007 t
ORIGIN      
        1 agcgcggtct tgggaattcg attttcctcg tgcaggttcg gcttgaccgg cattacgacg
       61 tcggccatcc gctcgtggcc ctcgtgcagg atcgggctgg acacctgccg cacggccgcg
      121 aaccgcggcg cgtagatgca gacgcagttg ctttcaacga tttcggtgcg gccgtcgagc
      181 gtgtcgaagt gggcgacggt gtcttcctga tcgaggccga cgacggtcca gtcctttttc
      241 acgtggacat cgtggttgag gtcgccgccg tcgcagatga attcgtcctg gggccaggga
      301 catttgaggc cgtcgggcat ccaggacaat tcttccgacg gcccctcgtc cgagaagtgg
      361 aacggcggca agcccgccgg gccgcagtgc ggacagtagc ctccgtccgc gtgtagcccg
      421 ccattccatg gcgggatgcc cgcgacggag tcgcgggcca cgccgccggt ccaggccgat
      481 ggcgggagcg tgtcgtgaac cggcaggctg tactgagcct gctgcacggg atagcggggc
      541 gcagccctgc tgacttgtcg cgtcggttca gccggcgcgc cgtctttctt ggcaatcaga
      601 aatgactgcc ccacgccttt gcacgagcaa aacagcacgg gcgccagcgc cagaaacaac
      661 agggtgtgtc gcatgcgttg cgtcctgcgt gagtgcttag ggcagccgcg gcggggcggg
      721 cagccgctct tccaggcctt gattgcgcgg cagggccggc ggcaattctt cgatgagctg
      781 caccggcggc tgatggtaga ggaagtggct ctgatcctcg tccggcagcg gcacgcgcga
      841 gcccatccgc aggatcgcga ccggccggcc caggcggtcg gccgtttcca tcgcatcgtc
      901 gcccggccgg atttcgaaat accgctgccg gcctggctga tcgcgaagcg gcagggccgt
      961 ccgcggctct tcgacgtaga tcacgcggag cacgtagcgg ccgtccaccg ccatcttgat
     1021 ttcctcttcg gtcagttcga ccgggatcgg aaagcgggcg gcctggcccg gcggcggata
     1081 gaggcggtcg atgacttcga tcgtcggaaa gatctccgtc ccttcgcggc cggggatgtt
     1141 gctgaccttc aggcgataga cctggccgat gagcatgccg gcgaggagct tctcgcgctt
     1201 ggcttccgag aattcgccct cgctgacaac cgagagcagc gtcgcagccg gggcggtcac
     1261 ttcgaccggc tgaaagtacc ccggcaaggg tccgccacgc tgaagctgcc gcaggccaat
     1321 cgctcccggc ggcatgtcgg ctttgttgag gtagtgccag ggcccttgag cggaggcgac
     1381 gggaagcccc atcgccgacg ccgcgagcca ggcggctacc gccgcgacct gccgaagatt
     1441 tgcgaccgca cgcagcatgg cggacctttc gtttcctgtg ggaacgcctc ggcggcgatc
     1501 ctgtagggaa cgccctccgt ggcgttcccg tgccggtccg ctcgcaatgc gagcggacgg
     1561 tcctcacgcg gggcgtgagg tcgactctag tgccccatct cttccggtgg gcagtagccg
     1621 gcgggcatgc cgccggcgcc gtgccggccg tggattgcac cgccctggac cggagctggc
     1681 actccaccgc gaaggccttc gtgcatgtcc cagtgcggct gctcgaaggg caggctcgga
     1741 tggatcatct gctcgttgat ccaggcccgg ttcgccggct tcggatagct ctggcccggc
     1801 tgctggcgga cgtggatctt cacgttttgt gtgggaccgg gaatgctcat atgcgtgtga
     1861 ttcttcatca cgtgcttctg caggccggcg gggccgccga agggaacgtg gggcggtcca
     1921 ggcaggccga tcggcgtgcc gacatagggc atgccgtact gcggcgcggt gatgccggcc
     1981 acgtagttgg gcacgaggcc cgacgcgccg aagggcatcg ggcaaggccc gccgaacccg
     2041 ccgccgaatc cgccgccctc gaacccgccg aggccctctt cggcgccggc cagctcgacg
     2101 tccttattgc ccagccgcac gacggccatg atcgagccgc ggcggtcggc ctcgacgatc
     2161 ggatcgacgc ccggatcgag ccgcgtgctg accagcgtat cgacgccggc cagggccagc
     2221 tcctggaact ccggatcggg caggtaaatc accttggtga cgaagttgcc ggcctgcacc
     2281 tggtcgaagt cttcctcggt gaactgcacc gggatcgcgt tgtgggccag gaaggcctcg
     2341 gtccgcggcg tggtcgggcc gatctcgacg gtcggataga gttccagccc cgcgcggccg
     2401 ggaatgtcgg tcagcttcag gcggtagagg ccgttctgcg ggaagttctg ccggccgggc
     2461 acgatcagcg gcgtgctgtc gaactgaccg acgccgccga cgtcccagcg aatctgcatc
     2521 gcctcgggcc gcgcgaagtt gacctgcacg ctcggcatcg cgacgggcat catcgccggc
     2581 ggcagcgacg gcggcagaac gcccggaccc ggacccccga cgcccggccc cggctccaag
     2641 agccgcggag ccggcggcaa gttgtgctgc ttcggaaacg cgcatcccag cgccgtgagg
     2701 cacacggcga gcgaagcgct cacaaggcac agacgaactt tcatgttacc cccctgctcg
     2761 cggcgcctcg aaaccgtccg ccgcgcggcg gccgagccga agcctgcttg gttgcgatgt
     2821 ttcgtgctag cggaccatcg tccgattgcc gtgggctcgt ttgccgtgga ccctgccgcg
     2881 cgcttgggct taagatgccg ctgccgatcg tccgatcgac aggcgcatca ccagcgccgg
     2941 cgtgggcggg ctatcgggcc acagggatat gttcggcgcg gtatctttcg attctttggc
     3001 aaaatcagca cgaccggcaa atgcggcgca gcttccccag tgtgatggtc agtgcccttt
     3061 gccttggctc ttggctcctg gcccctggtc cctggcccct cttccaggag ccagtcgcca
     3121 gtggccagga gccagcggcc ggcggtcagc agcttctggc cgaagccgtg cggcgactcg
     3181 atggccagac ggcgatttcc gccgagctgc gctatcgcgt gaatgcgttc gggcacgatc
     3241 ttgccggcac aggcagctac ttgcagctcg gcgctggccc gcagcggctg ctgcggctgg
     3301 agctgcggat gcaagtcggc gagcagccgg cgacgctcct ggaaattcgc ggaacggacc
     3361 gctattggat tcgccgcgac gtgccgccta atccgccgac gctcggccgc gtcaatctcg
     3421 tacagctgcg ccgcgggctg cggcgaccgg atcaaccggg cggcgacgat gctttgcccc
     3481 gcggcgcttg gatcatactg ggcggcttgc cgcgcttgct ggaggcgctg gagaatcact
     3541 tcgacttcgc cgctccgcgt gcggatgagg tattcgtcgc tgggagcggc ggcgagcggc
     3601 aagcgctgcc gatctgggtg ctcgacggca aatggaagcc cgaccggctc gccgcgctca
     3661 gcgggcgtca gtcgccggct gaaggcagct tgcccgagca gttgcccgat cgcgtcgaga
     3721 tcgtcctcga ccgctcggac cagagcctgc cgctgtttcc ctatcgcgtt tcctattgga
     3781 agtcggcgcc gcaaaccgct ggaaacgccg ccgcgcagcg tgagctgctg tcgctggagc
     3841 tcttcaacgt gcaccgctcc ggccagatcg acagccggca gttcgagtac catgccggcg
     3901 atcaggaggt cctggacctc accgcggcct atattcagcg gctcggcgga cctgcccggc
     3961 agcgctagcg gatggcaagc gcccgccgac tctgctatta gtaagtacgc gcttctcgcc
     4021 cgtcctctaa ctgcgctcgt gccatgaaag ctcgtgccat gaaactcact ttcagccttg
     4081 cactggctct gctgacgatt tccttgagcg ttcaggcagc cgacccgccg ctcaaggtgg
     4141 gcatcatcgg gctggatacg tcgcacgtca tcgccttcac taaaacgatg aacacgcccg
     4201 gcccgaaggc cgacgcgatc aactgccgcg tcgtggcggc ctatcccaag ggcagcccgg
     4261 acatcgaatc gagcaccagc cgcgtgccgg gctacatcga ggaaatgaag aagcacaacg
     4321 ttgagatcgt cgattcgatc gaggagctgc tgaaacgcgt cgattgcgtg ctcctggaga
     4381 cgaacgacgg gcggccgcac ttggagcagg ccctgccctg cttgaaggcc ggcaagccga
     4441 cgttcatcga caagccgctg gccggctcgc tggccgattg cctggccatc gtggacgcgg
     4501 cgaagaaata caactgcccg atgttcagct cgtcgtcgct ccgctacgga aagctgacgc
     4561 aggaagtccg cggcgggtcg ctaggcaaga tcaccagcgc ggaaacaacg ggctcggccg
     4621 cgctcgaaaa gacccatccc gatctcttct ggtacggcat tcacggcgtc gaatcgctgt
     4681 tcgccgtgat gggcacgggc tgcgagctgg tgacccgcaa gaccacggcc gacggcaaga
     4741 tcgaagtcgt cggcgaatgg gtcggcggcc gcaagggcac ctttcgcgag agccaaaaat
     4801 acggcggact cgccaagggt gacaagggcg aggggcccgt cggcgactcc gccggctacg
     4861 atccgctggt cctggagatc ggcaagttct tccattcgaa gaagccgccg atcccgcagg
     4921 ccgagacgct ggagatctac gctttcatgg aagctgccga cgagagcaag cgccgcgggg
     4981 gcgagcccgt cgagatcgag gaagtcatcg ccaaggccca gccgctggcg gccaagcgcc
     5041 tggcggagtt gggcgtgaag tagccggttt ttcgcaggac gtaagattag tgttcgtagg
     5101 atgcattggt tcgcggggcg ttgggtgcct gctcatcgag cgattgccga tagcgaatac
     5161 agccccgcag aaaattcggc cagtcgggca ctgactgcgt caaccattac ggccgcctct
     5221 gcacaattcc ggagaaactg cagaggggcg tcagcgcttg ggcagaaacc gtgcctcagg
     5281 tcatgtagac ggcgactccg cagatagggc acacaccgtc tttgacattc gaccgaaccg
     5341 tcaagccaca gcctgcgcag ttaccatatt ggcacagtcc gtagtcgggg ccgaggctgt
     5401 cgccccactt ggcaatattg ggataagcaa cggcgtccca tctccacctt ccccaccgct
     5461 cattctcgtt ctcgtcagac tcgaggtcgc tgcacgcaac acccaggaag aatcggctgc
     5521 ggcagaatgc ggcctcttcc gggaagggac atcgagtttc aaaaacatcg cgcaggaccg
     5581 ctaccaactc ttcaaccggg agttgcctga gatactcgct gatctgcttc cgatccattg
     5641 ttcaggcttg tgacccgggc aaaaaaggac agcgacacta aatccactgc atcgatgtct
     5701 cgcccgttgc gagtaattgg ccgcgacatc gggggcatgg catcccggat cgccatggcc
     5761 gaccgtcagg ccgtttgcac gagggacact gaccgaactc ttt
//
LOCUS       DASRJB010000603         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1586708, whole genome shotgun sequence.
ACCESSION   DASRJB010000603 DASRJB010000000
VERSION     DASRJB010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586708"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1154..2995)
                     /locus_tag="VFB80_15865"
     CDS             complement(1154..2995)
                     /locus_tag="VFB80_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35306.1"
                     /translation="MNLPAASHVRWAAAAIAVIGALAFASGFDGEFVLDDFIAIPHDS
                     QMHRVWPWGPIGDVNRWFGVWTFQLNYALGGNSPLGYHLVNIAIHVAAGLTLFDLARR
                     MLLLPGIPHALAERSTAVALIIALVWLVHPLQTQAVTYVIQRYESLMGLCFLLVLYCT
                     LRGAKGEHARRWYLAAATAAWAGAGSKEVMLACPLVLMLFDRAFLAASWREVLRRRGM
                     LHTAVLATGAWLIWASRSSLAAGGTSTAGFGFRGVTPWEYLRSQAGVILHYLRLAVWP
                     DELILDRGWPVAHAPLEIYGCGAIVLLLLFASIAAWFRWPRISWLGLAFFFVLAPTSS
                     INPIADLAVEHRMYLPLAAVVALAVLAIARLPVHGRFQWGLATLIIAALFVRTTLRNR
                     DYADPIGMWRQVVDHNPDHARGYRMLAAFLAQDGQLDQARRMYAASIEREPRIFQVWI
                     EYGNVFFQQGDYAAAIERYTRATRLNPKSYRAWMNIGRCQLRLGDYCAATSASRAALA
                     ALPDDAAACKQLAWLLATAPDAKVRDGAAALSLVDAIRQNPRRTDIQWHEVRAAALAE
                     LGRFDEATLEAARAVAAAESIRSKRLPELKLQLAGYQGHQPWRPSSH"
     gene            complement(2992..>4408)
                     /locus_tag="VFB80_15870"
     CDS             complement(2992..>4408)
                     /locus_tag="VFB80_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35307.1"
                     /translation="GATAARSWPWYAGAAAATWLGAGTKEVMAVCPLVLLLFDRCFLA
                     ATWRDVFRRRWALHLAVGLAAVWLVFTVRGTFVAAGATANAGFGYQGVSSWEYLRSQP
                     AVLLHYLRLAFWPDTLVLDYGWPVEHSAWRIYGWGAVIVALLGLSLLALARRPRLGFL
                     GISFFLVLAPTSSILPIADIAFEHRMYLPLAAVVTLAVLAAWQLIERLRLNEQRQRLL
                     GGGVLTLVVLALAARTIARNGDYCQPDRLWAKNIAAAPHHGRPYRLLAFHLRKAGRVD
                     EAKAALEQSLAREPNDYKTWVEYGSLFFGERDYDRALVSFQQAIGVQPRCALALGNIG
                     RIEMFRGNFSVALAASRSAAAADPNDPALRKQVAWLLATAPDAALRDGQEALRLIDSI
                     RQNPRKTDIQWHEVRAAALAELGRFAEAVVAIRRAALSAESIRSPRIAELKAQQSSYQ
                     DGRAWRLAPRLPQLVQGEARP"
BASE COUNT          802 a         1413 c         1503 g          690 t
ORIGIN      
        1 tccgtacgag ggctgcgtct cgctgctggt ccgcgaacag gaggccggcc ttcgacttct
       61 ccaggccgag gaaggcgcgg aggatccgga ggatcagcag gaagaaccac aaggttagct
      121 ggctcgttgc ccgccaacca cggtctggcc acattcccgc gccgtgtgaa cgggtcggcg
      181 agcggctcat ccccgccggc cgtctcgcac tccgctggcg catttcaccg cttgttgagg
      241 aaaggaaaag aacatggctc gaacacccct gcgactcccc ccgcaacgtg cgatcgctcg
      301 tccacagcat gctggagtgc tcgtggaggg gggcgtcact tcgcagttgc gcgtctgtac
      361 gccctgtgtc cgcgtcggcg gcggccagtg gtgctttacg ctccccgtca ttggccggcg
      421 gtgcctgagt gttccggccc tcggaacctg gaaggcctgc tgctcgctcc ggttcggctg
      481 gccgccggtg tcctgcggtc tcacccgctg ctaagtcgag ccgcaacggc gttgtaagcc
      541 ctctcccgtg cggagagggt cgaaggtagc ccatcgttga cagtaggtcg cgctgcgcgc
      601 ggcaggcgtc gccaaggtgt accttgcaaa aatgcgggca agtggcggta gtcgtgagtc
      661 gtatcttcct tcttgacaaa tagtggacaa cagatacaat tctctgctat cgctgccgct
      721 aggttggggc ggcgggctct ttggcatcct ggttgtatgc aacgcccaag tcgcagccgc
      781 ggctgctcgc ggcgttatgc cggatttggc cgctaagctg catattttgc atagcgggca
      841 tacggcataa acgtcgtaag tcgtttttct gccgtgactt gcgtcgaaat atgcggttgg
      901 aagaattccg cgattatgca tgcatgcgac tgccggtggc gaggctggag cctcggcagg
      961 cgtagatccc gccccccagc caaggcctgc tggcgcgcat aatgcgcata atggattgcc
     1021 ggcggatttc gacgtaactt attgcggaga ctggagttgc gatccgaatt gccgtccggg
     1081 gaaaacttgg cgatatgcgc gcatcagtat ggccgtgtgc cactgcgtca cgtgtcagga
     1141 attggcagga gggttagtgg ctggaaggcc gccagggttg atggccttga tagccggcga
     1201 gttgtagctt gagctccgga aggcgcttgc tgcggatgga ttcggcagcg gcgacggccc
     1261 gcgcagcttc gagcgtggcc tcgtcgaagc ggccgagctc ggccagggcg gcggcacgga
     1321 cttcgtgcca ttggatgtcg gtgcgccgcg ggttctggcg aatggcatcg accagagaca
     1381 aggcggcagc gccgtcgcgg acttttgcgt cgggcgcagt ggccagcagc caggccagtt
     1441 gcttgcaagc cgcggcgtcg tctggcagcg cagccagtgc cgcgcgcgat gcgctggtgg
     1501 ccgcgcaata atcgccgagc ctcagctggc aacggccgat gttcatccag gcccggtaac
     1561 tcttggggtt aagccgggtc gcccgcgtgt accgttcgat cgcggcagca taatcgccct
     1621 gctgaaaaaa gacgttgccg tactcgatcc agacctggaa aatccggggc tcgcgctcga
     1681 ttgaagccgc atacatgcgg cgggcctgat ccagttgtcc atcttgtgcg agaaacgcgg
     1741 ccagcatgcg gtagccgcgg gcgtggtccg gattgtggtc cacgacttgc cgccacatgc
     1801 cgatcggatc ggcataatcg cggttccgca gcgtggtccg cacgaagagc gcggcaatga
     1861 tcaatgtggc caggccccac tgaaaccgcc cgtgaaccgg caagcgggcg atggccaaca
     1921 cggccagcgc caccacggcc gccagcggca ggtacatccg gtgctcgacg gccaggtcgg
     1981 cgatcgggtt gatgctcgac gtcggggcga gcacgaagaa gaacgccagt ccgagccaac
     2041 tgattcgcgg ccagcggaac caggccgcta tcgaggcgaa aagcagcaag aggacgatcg
     2101 cgccacagcc gtagatttcg agcggcgcgt gggcgacggg ccagccgcgg tcgaggatca
     2161 gctcgtccgg ccagacggcc aggcggaggt agtgcaggat gacaccggct tgcgaacgca
     2221 ggtattccca gggcgtgacg ccgcggaagc cgaaaccggc ggtgctcgtg ccgcccgcag
     2281 ccaggctgct ccggctcgcc cagatcagcc acgcgccggt cgccagcaca gccgtgtgca
     2341 gcataccgcg gcggcgcagc acctcgcgcc acgaggcggc cagaaacgcg cggtcaaaca
     2401 gcatcagcac cagcgggcag gcgagcatca cttccttgct gcccgcgccg gcccaggctg
     2461 ccgtcgcggc tgccagatac cagcgccggg catgctcgcc ctttgcgccg cggagcgtgc
     2521 agtacagcac gaggaggaag cagagcccca tcagcgattc gtagcgctgg atgacgtagg
     2581 tgaccgcctg cgtctggagc ggatgcacga gccacaccag cgcgatgatc aaggcaacgg
     2641 ccgtgctgcg ctcggccagc gcgtgcggaa ttcccggcag cagcagcatc cgccgcgcga
     2701 gatcgaacag ggtcaggccg gcggcgacgt ggatcgcgat gttgaccagg tgatagccca
     2761 gcggcgaatt gccgccgagg gcatagttga gctggaaggt ccacaccccg aaccagcggt
     2821 tgacgtcgcc gatcggaccc cacggccaga cgcggtgcat ttggctgtcg tggggaatcg
     2881 cgatgaaatc gtcgagcacg aattcgccat cgaaaccgct ggcgaatgcc agggctccga
     2941 tcacggcaat cgccgcggcg gcccagcgga cgtgcgaagc ggcaggcagg ttcatggccg
     3001 cgcctccccc tggaccagtt gtggcaagcg cggtgccagc cgccatgcgc gaccatcctg
     3061 atagctggac tgttgagcct tcaactcagc gatccgtggg ctgcgaatcg actcggcgct
     3121 cagggcggct cggcgaatcg cgacgacggc ctcggcaaac cgccccagtt ctgccagggc
     3181 ggcagcgcgg acctcgtgcc actggatgtc ggtctttcgc gggttctggc gaatcgagtc
     3241 gatcagccgc agcgcctcct ggccgtcccg cagcgccgca tccggcgccg tggccagcag
     3301 ccacgcgact tgcttacgca gcgccggatc gttggggtca gccgcggctg cggagcgcga
     3361 agcggccagg gccacggaga aattgccgcg aaacatctcg atgcggccga tgtttcccag
     3421 ggccagagcg cagcgcggct gcacgccaat cgcctgctga aaggacacca gcgcccggtc
     3481 gtagtcccgc tcgccgaaga acaagctgcc gtactcgacc caggtcttgt agtcgttcgg
     3541 ctcgcgggcg agcgactgct cgagcgcggc cttggcctcg tcgacgcggc ccgccttgcg
     3601 aaggtgaaag gccagcaggc gataaggccg gccatggtgc ggcgcggcgg cgatgttttt
     3661 ggcccacagc cggtcgggct ggcaatagtc gccgttgcgg gcgatcgtgc gggcggccag
     3721 cgcgagcacg acaagcgtca gcacgccccc gccgaggagc cgctgtcgtt gctcgttcag
     3781 ccgcaagcgc tcgatcagct gccacgcggc cagcacggcc agtgtgacga cagccgccag
     3841 cggcaggtac atgcgatgct cgaacgcaat gtcggcgatt ggcaggatcg acgatgtcgg
     3901 ggcgagtacg agaaaaaacg agatccccag gaagcccagc cgcggccggc gggccagagc
     3961 cagcaagctg agtcccagga gagcgacgat cactgcgccc catccataga tccgccacgc
     4021 tgagtgctcg acgggccagc catagtcgag cacgagcgtg tccggccaga acgcgagtcg
     4081 cagataatgg agcagcaccg ccggctgcga ccggaggtat tcccacgagg agacaccttg
     4141 atagccaaag ccggcattgg ccgttgcgcc tgctgccacg aacgtgccgc ggaccgtgaa
     4201 gacaagccac acggcggcca ggccgacggc caggtgcaat gcccatcggc ggcgaaagac
     4261 atcgcgccac gttgctgcca gaaaacagcg gtcgaagagc agcagcacga gcgggcagac
     4321 ggccatgacc tccttagtcc ccgcgcccag ccacgtagcg gccgctgcac ccgcatacca
     4381 gggccagcta cgagcagccg tcgcgccg
//
LOCUS       DASRJB010000604         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1863932, whole genome shotgun sequence.
ACCESSION   DASRJB010000604 DASRJB010000000
VERSION     DASRJB010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863932"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFB80_15875"
     CDS             <1..865
                     /locus_tag="VFB80_15875"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35308.1"
                     /translation="FRPGESDRKADASRSPGTTGRRTALAEWIAAPDNPLPSRVLANR
                     LWQHHFGRGLCESPSDFGRLGEAPSHPELLDWLAGELLNNNWSMKRLHRLIVTSAAYR
                     RASHGPEVAAAAAKDPANKWLARMTVRRLSAEQIRDAAMAASGELDAKAGGQASDWTR
                     STRRAVYLKVLRNKQEPTLEVFDAPDGIFTTPVRNVTTTPTQSLYLINGPWMLQRAKS
                     FARRIGSDSSLTLEQRVATAYALAYGRPPTAEESRAALALVQNHSAQASDALTDWCHV
                     LLNSSEFLYVE"
     gene            875..2296
                     /locus_tag="VFB80_15880"
     CDS             875..2296
                     /locus_tag="VFB80_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35309.1"
                     /translation="MYLSRREMLQQAGTGFGALALAHLLARNLQSASPATSHQPLAAA
                     SARKPHHTPKAKSVIFLFMEGGPSHIDTFDPKPELDKLAGQPLPSSYKPVILAMGEKN
                     PPLLASKRNWAQYGEGGLWVSDWLPNMATCADDLCVVRSLYSDGLNHSGGVCQMNTGS
                     ILAGRPSLGAWVNYGLGTENDSLPAFVVMEDNPGAVVNGPRNWGAGFMPAAYQGTALA
                     GGEEPIKHLATPKDVTGERQRAKLALLDQMNRRYAAERPEQSELEARIASYELAFKMQ
                     AEAPEAVDLSQETAETRELYGLDQKETASFGRMCLFARRLVERGVRFVQLYSGSGSKW
                     DAHSGLEKNHSENCRSVDKPTAGLLQDLKRRGLLNDTLVIWGGEFGRTPMSEKGDGRD
                     HNPYGYTMVLAGGGVRGGRTVGSTDELGLHAVEDRLHTHDFHATLLHLLGLDHMQLIY
                     RHKNRPERPTLNEGTACAKIVAG"
     gene            2322..3317
                     /locus_tag="VFB80_15885"
     CDS             2322..3317
                     /locus_tag="VFB80_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35310.1"
                     /translation="MSDLSRRELLAYSATFGSAFLAAQGLASGEQQAAAPIAPAGKKY
                     AMQKSINLWALPYPQKMTLKECLQLCKDAGFDGVELNYALEGDLSPQTSEAGIKEIGA
                     MAEKIGIKISGVCSFLFWPYAFTHEDPERRKKGVELAGEMIRAARLLDTENLLVVPGS
                     VYAPWIPEEPPVPHDVALQRATAAVKSLIPEAAKHQVYLNMENIFANGFLHSPQEMNA
                     FVDGFANDRVKVHFDTGNIMQYHFPEHWIPILGKRIQNIHLKEYSKKLHEFNLNAFRL
                     LLDGTTNWPAVLAALEAAGYRGYLTFEYFNPFPHWPEAIVYHTSDALDRMLGRKA"
     gene            3317..3832
                     /locus_tag="VFB80_15890"
     CDS             3317..3832
                     /locus_tag="VFB80_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35311.1"
                     /translation="MNSRFLTIVVTLVFCCGVTAAVLLVGEFFRRRGAAIAGLKAPDS
                     LVLLAIDGAEDVEAKYTRAVSAGEEVFHQYAVLGQVEIADADERARIVAALQQAAVAP
                     DLEPPGNSFRPRHALIARENGQTIEIAISFESRQYHRYGQGREDYPAISRSAEAILTR
                     HLTDAGISLAP"
     gene            3911..>4779
                     /locus_tag="VFB80_15895"
     CDS             3911..>4779
                     /locus_tag="VFB80_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35312.1"
                     /translation="MNQRVENAQVPYLRTQLPGPRASQLIATDEQYTSPSYTRVYPLG
                     VERAYGAVIEDVDGNRFLDFTAGIAVCSTGHCHPRVVAAIQQQAAKLLHMSGTDFYYA
                     SQGDLAKKLAQLAPGGGARRVFFTNSGAESVEAAFKLARYHTGRQHMIAFFGAFHGRT
                     MGALSLTGSKVVQRRGFAPLIPLVSHIDYANPYRSEANCWQDSLSQLEDLFKRTVAPQ
                     EVAAIIVEPIQGEGGYIVPPVEFHRELRKLCDKHGILLVADEVQSGMGRTGKMFAMEH
                     FGVAPDIVCLAKG"
BASE COUNT          846 a         1626 c         1563 g          744 t
ORIGIN      
        1 gttcaggcca ggcgagagtg atcggaaggc tgacgcctcc cgctcgccgg gtacgaccgg
       61 ccgccgcacg gcgctggccg agtggatcgc cgcgcccgat aatccgctgc cgtcgcgggt
      121 gctggccaat cgcctctggc agcatcattt tggtcgcggc ttgtgcgaat cgcccagcga
      181 tttcggccgc ctgggtgagg cgccgtcgca tccggagctg ctcgattggc tggccggcga
      241 gctactgaac aacaactgga gcatgaagcg cctgcaccgg ctgatcgtga cctccgccgc
      301 ctatcgccgg gcgtcgcacg ggccagaggt cgctgccgcg gccgccaagg acccggccaa
      361 taagtggctg gcgcggatga ccgtccggcg gctctcggcc gagcagattc gcgatgcggc
      421 catggccgcc agcggcgagc tcgatgcgaa ggccggggga caggccagcg attggacacg
      481 cagcacgcgc cgcgcggtct acttgaaggt gctccgcaac aagcaggagc cgacgctgga
      541 ggtctttgac gcgcccgacg gcatcttcac gacgcccgtt cggaacgtaa cgacgacgcc
      601 gacgcaatcg ctgtacctga tcaacggccc ctggatgctg cagcgcgcca aatcgttcgc
      661 ccgccgcatc ggcagcgact cgtcgctcac gctcgagcag cgcgtggcca ccgcatacgc
      721 cctggcctat ggccggccgc cgaccgccga ggagtcgcgg gccgcgctgg cgctagtgca
      781 aaaccattcc gctcaggcca gcgacgccct caccgactgg tgccacgtgc tcctcaactc
      841 cagcgagttc ctgtacgtgg aataggagca gcagatgtac ctctcccgcc gcgaaatgct
      901 ccagcaagcc ggcaccggtt tcggcgcgct ggccctcgcc cacctgctcg cgcgcaactt
      961 gcaatcggct tccccagcca ctagccacca gccactagcc gctgcctccg cccgcaaacc
     1021 gcaccacact cccaaggcca agtccgtcat cttcctcttc atggaaggcg gcccgagcca
     1081 catcgacacg ttcgatccca agccggagct ggacaagctg gccgggcagc cgctgccctc
     1141 gtcgtacaag ccggtgatcc tggcgatggg cgagaagaat ccgccgctac tggcgagcaa
     1201 acgaaattgg gcccagtacg gcgaaggggg cctgtgggtc agcgactggc tgcccaacat
     1261 ggcgacctgc gccgacgacc tgtgcgttgt gcggtcgctc tattccgacg gcctgaacca
     1321 ctcgggcggc gtctgccaga tgaacacggg atcgatcctg gcgggccggc cgtcgctcgg
     1381 cgcgtgggtg aactacggcc tggggaccga gaacgacagc ctgccggcgt tcgtggtgat
     1441 ggaggataac ccgggcgccg tcgtcaacgg cccgcgaaac tggggcgcgg gattcatgcc
     1501 cgccgcctat cagggcacgg ccctggccgg cggcgaagaa ccgatcaagc acctggcgac
     1561 tcccaaggac gtgacgggcg agcggcagcg ggccaagctg gcgctgctgg accagatgaa
     1621 ccgccggtac gcggccgagc ggccggagca atccgagttg gaggcccgca tcgccagtta
     1681 cgagctggcg ttcaagatgc aagccgaggc cccggaagcc gtcgatctgt cgcaggagac
     1741 cgccgagacg cgggagctgt acggcctcga tcagaaagag accgccagct tcggccggat
     1801 gtgcctcttc gcccgccggc tggtcgagcg gggcgtgcgc ttcgtgcagc tctattccgg
     1861 cagcggcagc aagtgggacg cgcactccgg actggagaag aaccactcgg agaactgccg
     1921 ctcggtcgat aagccgacgg cgggcctgct gcaggacctc aagcgccgcg gcctcttgaa
     1981 cgacacgctg gtgatctggg gcggcgagtt cggccgcacg ccgatgagcg agaagggcga
     2041 cgggcgcgac cacaacccct acggctacac aatggtcctg gccggcggcg gcgtccgcgg
     2101 cggccgcacg gtgggctcga ccgacgagct gggcctgcac gcggtcgagg accggctgca
     2161 cacgcacgac ttccacgcca cgctgctgca cctcttgggt ctcgatcaca tgcagctgat
     2221 ctatcgccac aagaatcgcc ccgagcggcc cacgctcaac gaaggcacgg cctgcgcgaa
     2281 gatcgtcgcc gggtagccgc cctttctgct ggagaagcgc catgtcggat ttgtcccgcc
     2341 gcgagctgct ggcctattcc gcgacctttg gctccgcctt cctggccgcg cagggcctgg
     2401 cgtccggcga gcaacaggcc gcggcgccga tcgcaccggc gggcaagaag tacgcgatgc
     2461 agaagtcgat caacctgtgg gcgcttccgt atccgcagaa gatgacgctc aaggagtgcc
     2521 tgcaactctg caaggacgcg ggcttcgacg gcgtcgagct gaactatgcc ctggagggcg
     2581 acctctcgcc gcagactagc gaagcgggaa tcaaggaaat cggagcaatg gccgagaaga
     2641 tcggcatcaa gatcagcggc gtctgctcgt tcctgttctg gccgtacgcc ttcacgcacg
     2701 aggatcccga gcggcgcaag aagggggtcg aactggctgg ggaaatgatc cgggccgctc
     2761 gcctgttgga caccgagaac ctcctggtgg tgccgggcag cgtctatgcc ccgtggattc
     2821 ccgaagagcc gccggtgccg cacgacgtgg ccctccagcg ggcgacggcc gcggtgaagt
     2881 cgctgatccc cgaggcggcg aagcaccagg tttacctgaa catggagaac atttttgcca
     2941 acggctttct gcacagcccg caggagatga acgccttcgt cgatggtttt gcgaacgatc
     3001 gcgtgaaggt gcatttcgac accggcaaca tcatgcagta ccacttcccc gagcactgga
     3061 ttccgattct ggggaagcgg attcaaaaca tccacctcaa ggagtacagc aagaagctgc
     3121 acgagttcaa cctcaacgcc ttccgcctgc tcttggacgg cacgaccaac tggcccgccg
     3181 tgctcgccgc cctcgaagcc gccggctatc gcggctacct gacgttcgag tacttcaacc
     3241 cgttcccgca ctggcccgag gcgatcgtct atcacaccag cgacgccctc gaccggatgc
     3301 tggggcggaa ggcgtgatga actcgcgctt cctgacgatc gtcgtcacgc tggtattctg
     3361 ctgcggtgtc accgcggcgg tgctgctggt cggcgagttc ttccgccgac gcggggctgc
     3421 catcgccggg ctgaaggcgc ctgattcgct cgtcctgctg gccattgacg gcgcggaaga
     3481 cgtcgaggcc aaatacaccc gggccgtctc ggcgggcgaa gaggtctttc accagtacgc
     3541 cgtgctgggc caggtcgaaa tcgccgacgc cgacgagcgg gccaggatcg tcgccgccct
     3601 gcagcaagcc gccgtcgcgc cggacctcga gccgccaggc aacagctttc ggccgcggca
     3661 cgcactcatc gcccgcgaaa acggccagac gatcgaaatc gccatcagct tcgaatcccg
     3721 ccagtaccac cgctacggcc agggccgcga ggactatccc gccatttccc gcagcgccga
     3781 ggcgatcctc accaggcatc tgaccgacgc cgggatttcg ctggcccctt agccgccatt
     3841 ccgattgacc ttgctccccc cgcggtaagg cgtaggattt caaaggcgcg tgtgcgccgg
     3901 gagcagccgc atgaatcagc gagtggaaaa tgcgcaggtg ccgtacctgc ggacacaatt
     3961 gccggggcca agagcgagcc agttgatcgc gaccgacgag caatacacgt cgccgtctta
     4021 cacccgcgtc tatccgctcg gcgtcgagcg ggcctatggc gcggtcatcg aggacgtgga
     4081 cggcaaccgg tttctcgatt tcacggccgg cattgcggtc tgctcgacgg gccattgcca
     4141 tccgcgggtc gtggcggcga tccagcagca ggccgcgaag ctgctgcaca tgtcgggcac
     4201 ggacttctat tacgcttcgc aaggcgacct ggccaagaag ctcgcgcagc tggccccggg
     4261 cggcggcgcg cggcgggtct tcttcaccaa cagcggggcg gaatcggtcg aggcggcgtt
     4321 caagctggcc cgctaccaca ccggccgcca gcacatgatc gccttcttcg gggcgttcca
     4381 cggccgcacg atgggcgcgc tctcgctcac cggcagcaag gtcgtccagc gccgcggctt
     4441 cgccccgctg atcccgctgg tcagccatat cgattacgcc aacccgtacc gatccgaggc
     4501 gaattgctgg caggattcgc tcagccagct cgaggacctc ttcaagcgga ccgtcgcccc
     4561 gcaggaagtg gcggcgatca tcgtggagcc gattcagggg gagggcggct acatcgtgcc
     4621 gccggtcgag tttcaccgcg agctgcggaa actgtgcgac aagcacggca tcctcttggt
     4681 cgccgacgag gtgcagtcgg gcatgggccg caccggcaag atgttcgcca tggagcactt
     4741 tggcgtcgcc ccggacattg tctgcctggc taaggggat
//
LOCUS       DASRJB010000605         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1025350, whole genome shotgun sequence.
ACCESSION   DASRJB010000605 DASRJB010000000
VERSION     DASRJB010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025350"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFB80_15900"
     CDS             complement(<1..379)
                     /locus_tag="VFB80_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZN35313.1"
                     /translation="MILTGNEIKRHLGQDLVIDPFDESRLNPNSYNLTLHDELLTYEE
                     VVLDMAEPNRTRRLRIPKEGLVLTPQKLYLGRTAERTETHNFVPMIEGRSSIGRLGLF
                     VHVTAGFGDVGFCGYWTLEMFAIQ"
     gene            complement(508..1161)
                     /gene="nth"
                     /locus_tag="VFB80_15905"
     CDS             complement(508..1161)
                     /gene="nth"
                     /locus_tag="VFB80_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZN35314.1"
                     /translation="MPLASLAVRKKRAPQVVELLKGEYPNAECALVHDTPFELLIATI
                     LSAQCTDERVNIVTKELFAKCPTPAALAAVPIKQLERLIQSTGFFRNKAKNIHACCEK
                     LVAEHGGQVPAEMEKLIHLPGVGRKTANVVLGTAFRIASGVVVDTHVTRLSQRLGLTE
                     HEDAVKIERDLSAVLPQEEWIDFSHRLIWHGRRVCKARKPLCGSCVLERVCPKVGVE"
     gene            1247..1549
                     /locus_tag="VFB80_15910"
     CDS             1247..1549
                     /locus_tag="VFB80_15910"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HZN35315.1"
                     /translation="MASFHVTVPHALGQETARVRVEQFLAGVEREYAAHISDVRGQWS
                     DNTLQFGFVASGMKINGTLAVEPSAVEVSGPLPLMAALFRGRIEQTIREQLEKLLA"
     gene            complement(1603..2739)
                     /locus_tag="VFB80_15915"
     CDS             complement(1603..2739)
                     /locus_tag="VFB80_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35316.1"
                     /translation="MNGVNGERFNRLTWPAVVWFAACGIAAGQQTSAWKTGPQFRQQL
                     DAPLSLTWSERQLRDGLANLAQLVDVAILLDRRIDPNQTLELSASGESLQLVLAKIAA
                     PAKARVTAVGPCVYLGPPETAGKLATLAALRRQEASALPAEAKARLLRTQAWQWNELA
                     EPRQLLADLARQANVTTANPELLPHDLWPAQSLPPLPWVDRLSLLLAGFDLTFEIADQ
                     GKSIRLVAMPETAVLEKTYTTRADAVDLAAQLRRIVPEAKIRSEPGKLHVAASQDDHD
                     KVERLLSGQPVRTTTTTKAGAGGGEKRYTLTVENQPAGAVLRTVANSLGKELKYDTAV
                     LQKLKGTVTFTLKDATLDELLKKTLDPLGLTYKLDDAALEVVKP"
     gene            2908..3564
                     /locus_tag="VFB80_15920"
     CDS             2908..3564
                     /locus_tag="VFB80_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35317.1"
                     /translation="MTTPRRPTLQRLYHRYLETEESATFVRDVSRHYLLSTLQRLASG
                     GDCVSRRAAILAIGFLGDYSHNSVLGRALSDRDRGVRLLADSGIRQLWRRDGNDQQQR
                     LLGRLCRLNQSEQYEDAIRESSELIDEAPWFAEAWNQRAVGYFATRQFDAAASDCHQT
                     LELNEFHFGAAIGLAHCYLQLEDPFAALENFRRALGLNPDLEDVRGQIDVLERALEGR
                     "
     gene            complement(3569..4195)
                     /locus_tag="VFB80_15925"
     CDS             complement(3569..4195)
                     /locus_tag="VFB80_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35318.1"
                     /translation="MKQFGGARKGYLLESFFPTPGDLRAVLEELQPKLLITNTRVPRD
                     YVSTPIDEWLRAYERYADRAVSGRPVTWRLSSHLHISLSKSSERVGAWPVAGEPFKVL
                     EPRGPLVEFAPQLLFFESGRLSLSIYNLDNAAAFGLEIQCPRDSGRHLNQRLFQALCQ
                     RLRERSRPCVFVVGRKRLRPRLYISTDWGEEINRHSYLRKCKLRVELD"
BASE COUNT          755 a         1474 c         1416 g          826 t
ORIGIN      
        1 gctgaatggc gaacatctcc agcgtccagt agccgcagaa gcccacgtcg ccgaagccgg
       61 ccgtgacgtg cacgaagagg ccgaggcggc cgatgctcga gcggccctcg atcatcggca
      121 cgaagttgtg ggtttccgta cgctcggccg ttcggccgag atagagtttc tgcggcgtga
      181 gaaccaggcc ctccttcgga atgcgcagcc gccgcgtacg gttcggctcg gccatgtcga
      241 ggacgacttc ctcgtacgtc agcagctcgt cgtgcagagt gaggttgtag ctgttgggat
      301 tcagccgcga ctcgtcgaac ggatcgatga cgaggtcctg gccgagatgt cgcttgattt
      361 cgttgccggt caggatcatg ggcgcggggg gcgggagtca gggaacggga gtcggcggag
      421 gtcagaagtc agaggtcaga ggcccgcgaa tcttgccgca agcctatgac ctctgcactc
      481 tgcccctcat tccgcgctcc gcattcccta ttccacaccc accttcggac aaaccctttc
      541 cagcacacac gacccgcaca gcggctttct ggccttgcag acccgccggc cgtgccagat
      601 caggcgatgc gagaagtcga tccattcttc ctgcggcagg accgcgctca aatctcgctc
      661 gatcttcacg gcatcttcgt gctccgtcaa gccgaggcgc tgggaaagtc gcgtcacgtg
      721 cgtatcgacg acgacgccgc tggcgatgcg gaacgccgtg cccaggacga cgttcgccgt
      781 cttgcggccg acccccggca aatggatgag tttttccatc tctgcgggca cctggccgcc
      841 gtgctcggct acgagtttct cgcagcaggc gtggatattc ttcgccttgt ttcgaaagaa
      901 gcccgtgctc tggatcagcc gttcgagctg cttgattggg accgccgcga gcgccgcggg
      961 cgtcggacat ttggcaaaca gctccttggt gacgatgttc acccgctcat cggtgcattg
     1021 ggcggaaagg atcgtcgcga tcaaaagctc aaagggcgtg tcgtggacca aagcgcactc
     1081 ggcatttgga tactccccct ttagcagctc caccacctgg ggggcgcgct ttttgcggac
     1141 ggcgagcgaa gcaagcggca tggcggcggt tttccggagc cgtagaatgg cgggccggaa
     1201 ttctccctgt accgatttgc gactgtcaag tacgcgagcc aatcgcatgg catcttttca
     1261 cgtcaccgtc ccgcacgcgc tggggcaaga aacggcgcgg gttcgcgtcg aacaattcct
     1321 cgcaggcgtc gagcgcgagt acgccgcgca tatcagcgat gtccgcggcc agtggagcga
     1381 caatacgctg cagttcggct tcgtcgccag cggtatgaag atcaacggca cgctggcggt
     1441 agagcccagc gccgtcgagg tttccggccc gctgccgctg atggccgccc tgtttcgcgg
     1501 gcgcatcgag cagacgatcc gcgagcagct ggaaaagctg ctggcgtagg tgcggccggc
     1561 gtggacgccg gggctaagcg gcagacgcga atgtctgccg aactacggtt tcaccacctc
     1621 cagcgccgcg tcgtccagct tataggtcag ccccagcgga tcgagagtct tcttcagcaa
     1681 ctcgtcgagc gtcgcgtctt tcaaggtgaa tgtcacggtg cccttcagct tctggagcac
     1741 ggcagtgtcg tacttcaatt ccttacccag cgagttggcg accgtccgca gcaccgcgcc
     1801 ggcgggctgg ttctcgaccg tcagcgtgta gcgcttctcg ccgccgccag cgccggcctt
     1861 ggtggtcgtc gttgtacgta cgggctgccc ggagagcagc cgctcgacct tgtcgtggtc
     1921 gtcttgcgaa gcggcgacgt gcagcttgcc gggctcgctg cggattttcg cttccggcac
     1981 gatccgccgc agttgcgccg ccagatcgac tgcgtccgcg cgcgtcgtat aggtcttctc
     2041 caggacggcg gtttccggca tcgcgacgag gcggatgctc ttcccttggt ctgcgatctc
     2101 gaacgtcagg tcaaagccgg caagcagcag cgacagccga tcgacccacg ggagcggcgg
     2161 caggctttgg gcgggccaca ggtcgtgcgg cagcagctcg ggattcgcgg tcgtgacgtt
     2221 cgcctgccgc gccaggtcgg ccagcagctg ccgcggctcg gccagctcgt tccactgcca
     2281 ggcctgcgtg cggagcagtc gcgccttggc ttcagccggc aaggcggacg cctcctggcg
     2341 gcggagggcc gctagcgtcg ccagcttgcc agccgtttcc ggcgggccga gatagacaca
     2401 cggtcccacc gccgttacgc gggccttggc cggggccgcg attttcgcca gtaccagctg
     2461 caacgactca ccggaggccg acagctccag cgtttggttg ggatcgatcc gccggtcgag
     2521 aagtatcgcc acgtcgacga gctgcgcaag gttcgccagc ccgtcgcgga gctgccgctc
     2581 ggaccaggtg aggcttagcg gggcgtcgag ctgctggcga aactgcgggc cggttttcca
     2641 tgcgcttgtt tgctggccgg ccgcaatgcc gcaggccgcg aaccacacta ctgccggcca
     2701 cgtaagccga ttgaatcgct cgccattgac gccgttcatg tggagttccg ttaagctctg
     2761 ctggcagatt catttttgcc gcgcggcggc cttgcgccca ggtaagaacg gggcaaattg
     2821 ctcaccgcgc gaattgttgt cggcgcgtgc tccaattaaa ctcgaccctg acccgatgtg
     2881 cgtcctcagc cttcagcccc gcctcacgtg acgacacctc gccgaccgac gctccagcgg
     2941 ctctatcacc gctacctcga aaccgaggag tcggcgacgt tcgtccgcga cgtctcgcgg
     3001 cactacctgc tgagcacgct ccagcggctg gcgagcggcg gcgactgcgt ctcgcggcgg
     3061 gctgcgatcc tggccatcgg ctttctcggc gactattcac acaatagcgt cctcggccgg
     3121 gctctttccg accgcgaccg cggcgtgcgc ctgctggccg acagcggcat tcgccaactg
     3181 tggcgtcgcg atggcaacga tcagcagcag cggctgcttg gccgcctctg ccggctgaat
     3241 cagagcgaac aatatgaaga cgcgattcgc gagtcttccg agctgatcga cgaagctccc
     3301 tggttcgccg aagcctggaa ccagcgggcc gtcggctatt ttgccacgcg gcaattcgat
     3361 gcagcggcga gcgactgcca ccagacgctg gagcttaacg agtttcactt cggcgcggcc
     3421 atcggcctgg cccattgcta cctccaactc gaggacccat tcgccgccct cgagaacttc
     3481 cgccgcgccc tggggcttaa ccccgacctg gaagacgtgc gcggacagat cgacgtgctc
     3541 gagcgggcct tggaaggccg gtagccgtct aatccaactc aacccgcagc ttgcacttcc
     3601 gcaagtacga gtgccggttg atctcctcgc cccagtccgt gctgatgtaa agcctcggcc
     3661 gcaagcgctt tcgccccacg acgaatacac acggccgcga gcgctcgcgc agccgctggc
     3721 aaagcgcctg gaagagccgc tgattcagat gcctgccgct gtcccgcggg cattgaatct
     3781 ccaggccgaa cgcggctgca ttgtcgaggt tgtagatgct caggctcagc cggccggact
     3841 cgaagaagag cagctgcggt gcgaactcga cgagcgggcc gcgtggttcg aggactttga
     3901 agggttcacc ggcaaccggc cacgcgccta cccgctccga cgatttcgaa agcgaaatgt
     3961 gcaggtggct cgacagccgc caggtcaccg gccggcccga cacggcacgg tcggcgtatc
     4021 gctcgtaggc ccgcagccac tcgtcgatgg gcgtcgagac atagtcgcgc ggcacccgcg
     4081 tgttagtgat gagcagcttc ggctgaagtt cttccaggac cgcgcgcaag tcgcccggcg
     4141 tgggaaagaa cgactccagc aggtatccct tccgcgctcc accgaactgc ttcataggca
     4201 cctcatttct ccacgctgtc aattggctgc cagcataggt gatccgccgc atcggcatat
     4261 tccgcaaact cgatagcccg ggcacaaccg ctggttcaag ctcttccggc atcttgagat
     4321 tccctgctac caccgcgcca ggcaggccga cttcaagggc atggggacgg gcaaacgcgc
     4381 actcctcgcg gcggcagtct ttgcgctggc gttacagccg gtaaggtctg cgcctgcgca
     4441 atatccgtac cgcgtcatct accctgggca g
//
LOCUS       DASRJB010000606         8294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_740810, whole genome shotgun sequence.
ACCESSION   DASRJB010000606 DASRJB010000000
VERSION     DASRJB010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740810"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /gene="fliD"
                     /locus_tag="VFB80_15930"
     CDS             <1..1892
                     /gene="fliD"
                     /locus_tag="VFB80_15930"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZN35319.1"
                     /translation="ALDVDLDPADEPALKTLGNLLDRINAADPAKLAARISADGKRIE
                     LEDLTAGGGAFAVTSPVGGSLAEELGLTGAEFGGVIAGQRLISGLKTTLLGSLAGGDG
                     LGALGSIQLTDRSGQTATVNLAAAETLSDVIETINAAGIGITASYNSAHNGLLLTDTT
                     GSTASNLIVADGDATATATKLGLSANAAVAVVNSGSLARQTVSRNTLLSSYNGGRGIG
                     TGTFRITDSAGQAATVNLTLLEAQTIGDVIDAINALSIGVEARLNDAGDGIAIVDTAG
                     GSGALTIADNGVGKTAAGLHIAGAAVAGTIDGTTTHSVELDADDTLEDLVLKLNELDG
                     GFSASVFDDGAGSLRYHLSLLGGVVGAAGQLVIDGSELGLQFQDVSTAQDALLQIGGG
                     STAGILVSSPTGKFDDVHPGLDTTLLAADGTTVTVSVSQTAEDVASALQLFVDQYNKL
                     QEKLETYTAFDLAAGTKGTLFGTSEAVRLDSELSRLVTGRYFNDGQIRSLAELGVSID
                     DQGKLTLDKLKLEEKFNADPEAVSEFFADEERGFAPLADAALERLVGIDRSLLINRTA
                     ALQRKIEDFGGRIDAWNGRLDRSRQRLLLQFFRLEQVVGRIQNNLTAINQIQLIKPIP
                     VDSSN"
     gene            1911..2408
                     /locus_tag="VFB80_15935"
     CDS             1911..2408
                     /locus_tag="VFB80_15935"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZN35320.1"
                     /translation="MELSARDAYLETQVNTATPQRLRLMLIEGALRQAQLAKTAWQES
                     RPAGAAAALIGCRDIVVELLSGISPGDTPLVRQTLGIYAYLYSSLTELQQSRDAHELA
                     GIIRVLEEEQQTWQEVCLALPHRVDTRAAVEERAPPRVGQALQPSYGGPAASSAPIAS
                     MSIEA"
     gene            2462..3850
                     /locus_tag="VFB80_15940"
     CDS             2462..3850
                     /locus_tag="VFB80_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN35321.1"
                     /translation="MGFAQWFWRLFLVFAGLLLAHVLTVSLIVSVQRNSAAGPVTWPI
                     WLAGGVALACGGVAIWYCVRQIVEPLVELSEQARAASAGHLTPPPGVDGHDEVGLLAG
                     AFNRMQRDLDHRHVQIEQNNQRLQAVLGSMAEGVLAVSPDHTILLANEAARRLLDFAA
                     REPLGRPLLEVTRARPVYSAVQQAMTTAAAVELEFEAPGLPRRALLLRATRLPGAPSP
                     GVMVVLHDLTELRRLENLRRELVANVSHELKTPLAAIKGYAETLRLGAVNDPEHNLHF
                     VRRIEEQADRLHELILDILQIARLESGQETFELADVPLAQVIEQCCEQFAETAAGRQV
                     ALKFELPPAEWRVRADDEGVRTILSNLIDNALKFTPAGGAVTVRSSANGAFATIEVAD
                     TGIGIAPHDQERIFERFYRADKARSRDLGGTGLGLSIAKHLAQAFGGNVSVVSRPGQG
                     STFTVELPRPAA"
     gene            complement(4263..5963)
                     /locus_tag="VFB80_15945"
     CDS             complement(4263..5963)
                     /locus_tag="VFB80_15945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35322.1"
                     /translation="MTSSPFTAPQAAEHEQQLALVVSELTDRVQRGEAVDLEAECRRH
                     PGLAQDLRELWGVIAVARAAGSNSALTAAGTALTLPPGSPGDFPSGHLSLPARFGDYE
                     LREELGRGGMGVVYRAAQTSLGREVAVKMILRGQLASKADRERFEAEAQAAARLDHPG
                     IVPVYEVGEINGRPYFSMKHVRGTTLAQRLADGPLPPREAARMLAAVARAIHFAHMRG
                     VLHRDLKPANILLDEQGEPHVTDFGLAKQLTDAATLTKTGAVLGTPAYMAPEQASGHR
                     GQVGPASDVYSLGVILYHMLTGRPPFQAASPGEMVLLVLEQDPVPPRMLNPKADRDLE
                     MICLRCLQKPIDLRYASAAALADDLEAYLNDESISARSGRFAQVLAGLMRETHHAAVL
                     ENWGLLWMWHSLVLLIACVATNLLYLMGVQNRWSYFLLWTAGLGAWAGVFWALRRRMG
                     PVTFVERQIAHLWAGSMICIALLFPLEAWLDFPPLKLSPVLGLVTGMVFLAKAGILSG
                     EFYVPAVALFATAGLMAYIPQYAHFIFGVVSACCFFFPGLKYYRQRLRAIVSALRGSS
                     "
     gene            6240..7739
                     /gene="ffh"
                     /locus_tag="VFB80_15950"
     CDS             6240..7739
                     /gene="ffh"
                     /locus_tag="VFB80_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZN35323.1"
                     /translation="MFESLQTGLRSALKTLQGKARLSEANMRDGLALVEQSLLEADVS
                     YSVAKDFIANISERALGEKVLLALNPTQQLIGLIHQELINVLGPVDPAIPLRKDVTTL
                     MMCGLQGSGKTTTCGKLAKMLDQYKKKCLLVAADLQRPAAIEQLHVIGRQLEVAVYSE
                     PGAKDPVAVCTNAVQKARADQIPVVILDTAGRLAIDQELMDQLVRIDKRVSPDQVYLV
                     VDGMTGQDAVNSAKAFNEALELDGVIMTKLDGDARGGALLSVKHVTGVPIKFIGTGEH
                     LDALEPFRPEGMAGRILGMGDVLGLVDTVQRAVDFEKQKELEAKLAAGEFTLDDFRGQ
                     MRQLMQPGLMQKMLGLLPGGGQLLEMMGAGGEDAEGGMGRMLGIIDSMTPQERRSPKI
                     VDPSRRNRIARGAGVQVQEVNQLLKQFDMMAPIMKTMAGKGMGQRMQALQQLQNSGMF
                     NPGARAPKVKGDTGKRLTSKEKAELRKKREKELKKKKREQGKQGPTGGW"
     gene            7883..>8294
                     /gene="rpsP"
                     /locus_tag="VFB80_15955"
     CDS             7883..>8294
                     /gene="rpsP"
                     /locus_tag="VFB80_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZN35324.1"
                     /translation="MAVKIRMKKLGRNHQPFFRMCAIDSKAPRDGKTIEELGHYDPLV
                     KDVDARAILNGERIAYWLSVGAQPSENCAVLIKKYGKDGTHVAQQQAALERIKTTRPQ
                     APAPYIPPPKPPEPEPQAEAPPETPPAEQPPEAPA"
BASE COUNT         1571 a         2738 c         2702 g         1283 t
ORIGIN      
        1 cggccctcga cgtcgatctc gatccggccg acgagccagc cctgaaaacg ctcggcaact
       61 tgctcgatcg aatcaacgcc gccgatccgg cgaagctcgc ggcccgcatt agtgccgatg
      121 ggaagcggat cgagctggaa gacctaactg ccggcggcgg cgcgttcgcg gtgaccagcc
      181 ccgtcggcgg cagcctggcc gaagagctgg gtctgaccgg cgcggaattc ggcggagtca
      241 tcgcgggcca gcgcttgatc tcgggcctca agacgacgct cctcggctcg ctcgcggggg
      301 gcgacggcct gggtgcgctt gggtcgatcc agctcaccga ccgcagcggc caaacggcga
      361 cggtcaacct cgcggccgcc gagacactca gcgacgtcat cgagacaatc aacgctgccg
      421 gaatcggcat caccgcctcc tacaactcgg ctcacaacgg tctgctcttg accgatacca
      481 cgggctcgac cgccagcaac ctgatcgtcg ccgacggcga cgccaccgcc acggccacga
      541 agcttggttt gtcggccaac gccgcagtcg ccgtcgtcaa ctccggctcg ctggcccgcc
      601 agacggtcag ccgcaatacg ctgctcagct cgtacaacgg cggccgggga atcggcacgg
      661 gcacgtttcg gatcaccgat tcggcgggcc aggccgccac ggtgaatttg accttgctgg
      721 aagcccagac gatcggcgac gtgatcgacg cgatcaacgc gctatcgatc ggggtggagg
      781 cccgcctgaa cgacgccggc gacggcatcg ccatcgtgga cacggcgggc ggcagcggcg
      841 cgctcacgat cgccgacaat ggcgtcggca agaccgccgc cggcctgcac atcgccggcg
      901 ccgcggtggc cgggacgatc gacggcacca cgacgcactc cgtcgaactg gacgccgacg
      961 acaccctcga agatctggtc ctgaagctga acgaactgga cggcggcttc agcgccagcg
     1021 tcttcgacga cggcgcgggc tcgctgcgct atcacctctc gctgctgggc ggcgtcgtcg
     1081 gcgcggcggg ccagctggtc atcgacggca gcgagctggg cctgcaattc caggacgtct
     1141 cgacggccca ggacgccctg ctgcaaatcg gcggcgggag cacggcggga atcctcgtct
     1201 cgtctcccac cggcaaattc gatgacgtgc atccgggcct ggacacgacg ctgctcgccg
     1261 ccgacggcac cacggtgacg gtgagcgtct cccagaccgc ggaagacgtg gccagcgcgc
     1321 tgcagctctt cgtcgatcag tacaacaagc ttcaagaaaa gctcgaaacc tacacggcgt
     1381 tcgacctggc ggccggcacc aagggcacgc tcttcggtac gtccgaagcg gtgcggctgg
     1441 acagcgagct gtcgcgcctg gtcaccggcc ggtatttcaa cgacggccag atccgctccc
     1501 tggccgagct gggcgtttcg atcgacgacc aggggaaact gacactcgac aagctgaagc
     1561 tcgaagagaa gttcaacgcc gatcccgagg ccgtcagcga attcttcgcc gacgaggagc
     1621 gcggctttgc tcccctggcc gacgccgccc tggaaaggct cgtcgggatc gaccgctcgc
     1681 tgctcatcaa tcgcaccgcg gccctgcaac gtaaaatcga ggacttcggc ggccggatcg
     1741 acgcctggaa cggccggctc gaccgcagcc gccagcgcct gctgctgcaa ttcttccgcc
     1801 tcgagcaggt cgtcggtcgt atccagaata atctgacggc catcaatcaa atccagctca
     1861 tcaagccgat cccagtggat agctcgaact agcttgcttg ggagccggcc atggaactct
     1921 ccgcccgcga cgcctacctc gaaacgcagg tgaataccgc cacgccacag cgcctgcggc
     1981 tgatgctgat cgaaggcgcg ctgcggcagg cgcagctggc aaaaacagcc tggcaggaat
     2041 cgcgccctgc tggcgctgcc gccgcgctca ttggctgccg cgacatcgtg gtcgagctgc
     2101 tctcggggat cagcccgggc gatacgccgc tcgtgcggca aacgctgggc atctatgcct
     2161 acctgtattc gtcgctgacc gagttgcagc agagccgcga cgcgcacgag ctcgccggta
     2221 tcatccgcgt gctggaggaa gaacagcaga cctggcagga agtctgcctg gcgctgccgc
     2281 accgcgtcga tactagggcg gccgtcgaag agcgcgcacc gccgcgcgtc gggcaagccc
     2341 ttcagcccag ctacggcgga ccggctgcat cgtccgcgcc gatcgcaagc atgtcgatcg
     2401 aggcgtaaca cggcggccct tcccttttca gttatttgcc gccaccttag gctggcggct
     2461 tatgggcttc gcgcaatggt tctggcgatt gttcctcgtc ttcgctgggc tgctgctcgc
     2521 ccacgtgctc acggtttcgc tgattgtctc ggtgcagcgg aactcggcgg ctgggccggt
     2581 gacgtggccg atctggctgg ccggcggggt ggcgctcgcg tgcggcggtg tggccatctg
     2641 gtattgcgtg cggcagatcg tcgagcccct ggtcgagctg agcgagcagg ctcgggccgc
     2701 gtcggcgggc catctcactc cgccgccggg cgtcgatgga cacgatgaag tgggcctgct
     2761 ggccggcgcc ttcaaccgga tgcagcgcga cctcgaccac cggcacgtgc agatcgagca
     2821 gaacaaccag cgactgcagg ccgtgctggg gagcatggcc gagggcgtgc tggccgtcag
     2881 ccccgaccat accatcctgc tggccaacga ggccgcccgg cggctcttgg atttcgcggc
     2941 ccgcgagccg ctcggccgcc cgctgctcga agtcacccgc gcgcgaccgg tctattcagc
     3001 agtccagcag gcgatgacca ccgcggcggc cgtcgagttg gaattcgaag cgccgggcct
     3061 gccgcggcgg gcgctgctgc tgcgggcgac gcgcttgccc ggtgcaccca gtcccggcgt
     3121 gatggttgtg ctgcacgacc tgaccgagct gcggcgcctg gagaatctcc gccgcgagct
     3181 ggtggccaat gtctcgcacg agctcaagac cccactggct gccatcaagg gctatgccga
     3241 aacgctgcgg ctgggcgcgg tgaacgatcc tgaacacaac ctccatttcg tccgccgcat
     3301 cgaagagcag gccgatcgcc tgcacgagct gattctcgac atcctgcaga tcgcgcggct
     3361 ggagtcggga caggagacgt tcgagctggc ggacgtgccg ctggcccagg tgatcgaaca
     3421 atgctgcgaa caattcgccg agactgcggc cgggcggcag gtcgcactaa agttcgagtt
     3481 gccgcctgcg gaatggaggg tccgcgccga cgatgagggg gtccggacaa ttctcagcaa
     3541 tctcatcgac aacgcgctta agttcacgcc ggccggaggc gccgtcacgg tccgcagcag
     3601 cgccaatggc gccttcgcca cgatcgaagt cgccgacacg ggcatcggca tcgcccccca
     3661 cgaccaggag cggatcttcg agcggttcta tcgcgccgac aaggcccgct cccgcgacct
     3721 gggcggcacc ggcctgggcc tctccatcgc caagcacctg gcccaggcct tcggcggaaa
     3781 cgtcagcgtc gtgagccggc ccggtcaagg ctcaacgttt accgtcgagc tgccacgccc
     3841 cgccgcctaa gcgcgatcct ccatttcttg acaatagtga acggattagt acaatcaccc
     3901 gcttccgcgc gccgcaaccg ctgcgatgag ccgtgatcac ttcatgctgc attggtccgg
     3961 cttaagttgc ataaactgca tacggatgca ccgtaccgtg cggcgtaaat acaattccgg
     4021 acactactta caaccagaaa tggccgccca aaaaaacggg aatatgcgtg catacgcgaa
     4081 ggcatcgcag aatggccctt tttccctcgt ttctttcagt tcgcgtcgcc gccacgagaa
     4141 tgcagcgctg caaaacgtgc atatgcgaca tcgcgaatct gcgtcacaag ttgttgtgag
     4201 ggaatgactt gcggcaaatc atgcagtcga gagattccgc aattatgcat gcatatatgc
     4261 attcacgaac tgccgcgcag ggcgctaaca atggccctga ggcgctgccg gtagtacttc
     4321 agcccgggaa agaaaaagca gcaggcgctg acgacgccga agatgaagtg cgcgtattgg
     4381 gggatgtagg ccatcaagcc ggccgtggca aacaaggcca ccgccgggac atagaactcg
     4441 cccgacagga tgccggcctt ggccaggaag accatgccgg tgaccagtcc cagaaccggc
     4501 gagagcttga gcggcggaaa gtcgagccag gcttccagcg gaaacagcag ggcgatgcag
     4561 atcatcgaac cggcccagag atgggcgatc tgccgctcca caaaggtgac cggccccatc
     4621 cgccgccgca gggcccagaa gactccggcc caggccccca ggcccgcggt ccagagcagg
     4681 aaatacgacc agcgattctg cacgcccatc aagtagagca agttcgtggc aacacaggcg
     4741 atgagcagca ccaggctgtg ccacatccag agcaggcccc agttttcgag caccgcggcg
     4801 tgatgcgtct cgcgcatcaa gccggccagc acctgggcaa atcgcccgct gcgggccgaa
     4861 atgctctcgt cgttgagata ggcctccagg tcgtccgcga gggcagccgc cgacgcgtag
     4921 cgcaggtcga tcggcttctg caggcagcgg aggcaaatca tctccaggtc gcggtcggcc
     4981 ttgggattga gcatccgcgg gggcaccgga tcctgctcca ggacgagcag caccatctcg
     5041 ccgggcgagg cggcctgaaa cggcggccgg ccggtgagca tgtgatagag aatgacgccc
     5101 agcgaataga cgtcgctggc cgggccgacc tgcccgcggt gaccgctggc ctgctccggc
     5161 gccatatagg ccggcgtccc caggaccgcg ccggtcttcg tcagagtcgc ggcatcggtg
     5221 agctgcttcg ccaggccgaa gtcggtgacg tgcggctccc cctgctcgtc gagcaggatg
     5281 ttcgcgggct tgaggtcgcg gtgcagcacg ccccgcatgt gggcgaagtg gatcgcgcgg
     5341 gcgaccgcgg ccagcatccg cgccgcctcg cgcggcggca gcggcccgtc ggccagccgc
     5401 tgggccagcg tcgtgccgcg gacgtgcttc atgctgaaat acggccggcc gttgatctcg
     5461 ccgacttcat agacgggcac gatgcccgga tggtcgaggc gggctgcggc ctgagcttcg
     5521 gcctcgaacc gctcgcggtc ggccttcgag gcgagctgcc cgcggaggat catcttcacg
     5581 gcgacttcgc ggcccaaact ggtctgtgcc gcccgataga ccacgcccat gccgccgcgg
     5641 cccagctcct cgcgcagctc gtagtcgcca aaccgcgccg gcagcgacag gtggccgctg
     5701 ggaaagtcac cgggcgatcc cggcggcagc gtcaaagcgg ttcccgccgc agtgagcgcg
     5761 gagttgctgc ccgccgcccg ggcgactgcg atcacgcccc acagctcacg caggtcctgg
     5821 gccagccccg gatgccgccg gcactctgcc tccaagtcca ccgcttcgcc ccgctgcacg
     5881 cgatcggtca gctccgacac caccagggcc agctgctgct cgtgctctgc cgcttgcggc
     5941 gcggtgaacg gcgacgacgt catgcttcga tcctactcgc ggcgctatac ggcgaacaac
     6001 gtccgaccct gtgcagccgc agcttcgcat taacgcaaag acacgaaggg gcaaaggcgc
     6061 agcacctttg ttcctttgcg ttgaaagcga ggaacgccgc agcacatgcc cgcgccgagc
     6121 gtgacctagg cacacgtcgc cgggatttac agtggcaacc atcattcgct agaatattgg
     6181 gtttccacgc gcggcaagat cacttgactt tgccgtaagt gttgatttga caagcagtta
     6241 tgttcgagtc cctccagacc ggtttgcgct cggcgctgaa gaccttgcag ggcaaggcgc
     6301 ggcttagcga agccaacatg cgcgacggcc tcgcgctggt cgagcagtca ctgctggagg
     6361 cagatgtcag ctacagcgtc gccaaggact tcatcgccaa catctccgaa cgggcgctgg
     6421 gcgagaaagt gctgctggcc ctcaatccga cgcagcagct catcggcctg attcatcagg
     6481 agctgatcaa cgttctgggg ccggtcgatc cggcgatccc gctccgcaag gacgtgacga
     6541 cgctgatgat gtgcggcttg caaggttcgg gcaaaacgac gacctgcggc aagctcgcca
     6601 aaatgctcga tcagtacaag aagaaatgcc tgctcgtcgc cgccgacttg cagcggcccg
     6661 cggccatcga gcagttgcac gtcatcggcc gccagctcga agtggcggtc tattcggagc
     6721 cgggcgcaaa agacccggtc gcggtctgca ccaacgccgt gcagaaggcc cgcgccgacc
     6781 agattcccgt cgtgatcctc gacacggccg gccggctggc gatcgaccag gagctgatgg
     6841 accagctcgt gcggatcgac aagcgggttt cgcccgacca ggtttacctg gtcgtcgatg
     6901 ggatgaccgg ccaggacgcc gtgaacagcg ccaaggcctt taacgaggcg ctggagctgg
     6961 acggcgtgat catgaccaag ctcgacggcg acgcccgcgg cggcgcgctc ttgagcgtca
     7021 agcacgtcac tggcgtgccg atcaagttca tcggcaccgg cgagcacctc gacgccctgg
     7081 agccgttccg ccccgaagga atggcgggcc gcatcctcgg catgggcgac gtgctgggcc
     7141 tggtcgatac ggtccaaaga gccgtcgatt ttgagaagca gaaagagctg gaggccaagc
     7201 tggccgccgg cgaattcacg ctcgacgact tccgcggcca gatgcggcag ctgatgcagc
     7261 cgggcctgat gcagaaaatg ctgggtctgt tgccgggcgg cggccagttg ttggagatga
     7321 tgggcgccgg cggcgaggac gcggaaggag gaatggggcg gatgctgggg atcatcgact
     7381 cgatgacgcc ccaggagcgg cgcagcccga agattgtcga tcccagccgc cgcaaccgga
     7441 tcgcccgcgg ggccggcgtg caggtgcagg aggtcaatca gctgctcaag cagtttgata
     7501 tgatggcccc gatcatgaag acgatggcgg gcaaggggat gggccagcgg atgcaggccc
     7561 tccagcagct gcaaaacagc ggcatgttca atcccggcgc tcgcgcgccc aaggtcaagg
     7621 gcgacaccgg caagcggctg acttccaagg agaaggccga gttgcgcaag aagcgcgaga
     7681 aagaactgaa aaagaagaaa cgcgagcagg gcaagcaggg accaacgggc ggctggtgat
     7741 gcggagaaat ccgaagaacc aaagaaccga agaaccaaac aaagcaattg aggacgatca
     7801 acagaagaac agggaacttg gaaccgcgat ttttggttct ttggttcttc ggttctttgc
     7861 atttttgctt ccggaggtat cagtggcagt caagattcga atgaagaagc tggggcgcaa
     7921 tcaccagcct ttcttccgca tgtgtgcgat cgacagcaaa gcgccgcgcg acggcaagac
     7981 gattgaggag ctgggccatt acgatccgct cgtcaaggac gtcgatgccc gcgcgatcct
     8041 caacggcgag cggatcgctt actggctctc ggtcggcgcg cagccgtcgg agaactgcgc
     8101 cgtgctgatc aagaagtacg gcaaggacgg cacgcacgtg gcccagcagc aggcggccct
     8161 ggagcggatc aagacgactc gcccgcaggc ccccgcgccg tacattccgc cgcccaagcc
     8221 tccggaaccg gagccgcagg ccgaggcgcc accggaaacg ccgcctgccg agcaaccgcc
     8281 agaagcgccg gcag
//
LOCUS       DASRJB010000607        17230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2432017, whole genome shotgun sequence.
ACCESSION   DASRJB010000607 DASRJB010000000
VERSION     DASRJB010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17230
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432017"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..706)
                     /locus_tag="VFB80_15960"
     CDS             complement(173..706)
                     /locus_tag="VFB80_15960"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN35325.1"
                     /translation="MTFLHLQAALVLLFVIIAAALDLRTKKIPNWITVPAALAGLVFH
                     AAAPAGSGILCALAGFAVGFSLLLLPWLLGGGGMGDVKLLAALGVWLGPLTILIVFGA
                     SAVIGAVMAAGVMVGGMCTRGISATRRRYLNVDEDRETAAKNQPRGRRVIPFAVPIAI
                     STVVVLALMLVKASVQP"
     gene            complement(1063..1362)
                     /locus_tag="VFB80_15965"
     CDS             complement(1063..1362)
                     /locus_tag="VFB80_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35326.1"
                     /translation="MKRTLLALWREDDGALSFEWCLVAVLLVFGIVGGLSAARDGILD
                     ELSDVAASVLNFDQSFSFAGVDCIPESVFEDDPGTVVDCARNTLPGQPAVDDVES"
     gene            complement(1403..1723)
                     /locus_tag="VFB80_15970"
     CDS             complement(1403..1723)
                     /locus_tag="VFB80_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35327.1"
                     /translation="MKRTVVALWREEDGILSFEWTMVLTLLVIGIVAGLSAARDAIID
                     ELGDVAQAAGAIDQSYSLAEIDIVDPPIFAPGMSFTDDAMVYTDCARELGPGGQTALS
                     DNGS"
     gene            complement(1819..2169)
                     /locus_tag="VFB80_15975"
     CDS             complement(1819..2169)
                     /locus_tag="VFB80_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35328.1"
                     /translation="MKRLAWQIWQQQDGVLSFEWTLVLTLLVIGVVAGLSAARDAVID
                     ELGDAAQAMLALDGSFVISQPLAMSIDSDGAGPGAPVAVGGASDSAFTDRATYADCDR
                     SELAGQAALSDDGS"
     gene            complement(2256..2582)
                     /locus_tag="VFB80_15980"
     CDS             complement(2256..2582)
                     /locus_tag="VFB80_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35329.1"
                     /translation="MKVLSRMWKEEDGVLSFEWVMLVTLLTIGIVSGLTGARDAIIDE
                     LGDVAQAMLALDQSYTISFPLDVDVHIDDPSSASDSGFTDAALFVDCAREEAPPGQDA
                     LTDGTS"
     gene            3260..4690
                     /locus_tag="VFB80_15985"
     CDS             3260..4690
                     /locus_tag="VFB80_15985"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN35330.1"
                     /translation="MARDDNPFGPQPKPTATSQGILTLPAFWLGLSRDVSQVSYAATG
                     LGLMAAKYAVEAGLIYHYTGRFFSPLDFLNPLLSMREQFLRAPAPPWLGWSLFLWSLP
                     FVWIAVSMSVRRSANAGATAWLGLLVLVPLVNYVVMLVLCLLPSRASFTWQGERPRPV
                     VDHLVRSALLGILVSVAISISMILLCVYVLADYGAALFLGTPILVGAVSSFIYNRPYP
                     RDLGSSLVVAELAISLSGAAILLFAFEGIICLFMLLPIAGIMGLLGGLIGYAVASLTP
                     HRLALLLLPILILPLAAGAERVHRPTPLYEVISSVEIDAPPEKVWPHVVGFSELPAPP
                     AWYFRLGIAYPKRATIEGSGVGAVRRCEFSTGPFVEPITVWDQPRRLAFDVQSQPPPM
                     HELSPYRHVHPPHLDGYLRCRRGEFRLIELPGGRTRLEGSTWYEFEMYPQDYWTLWSD
                     TCIHQIHRRVLDHIKVLAEEDMKGIE"
     gene            4816..6372
                     /locus_tag="VFB80_15990"
     CDS             4816..6372
                     /locus_tag="VFB80_15990"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZN35331.1"
                     /translation="MFAFCGNGRRLLVAAAMSCVSLAVWGAAANAEEAKKIDGRELFT
                     REWIPGDARSHGGDGLGPVFNDTSCVGCHNQGGVGGAGPKAKNAQIVTAFSNRAVNQG
                     FEPASTSLFGAIITMFGGPRTASPRPPATEDEADRIAKERDDLAKIHPGFRTARSVVL
                     HRASTESEYAAFRGKLNGMANFEGMVGNIAMEGRIVPAGESGPPLSVEQQSEINRLKA
                     EAQFGGQQAFNGGAGNFTFLVSERNTIPLFGSGKIDVIPDNVLIAAAEQRFDDFPEVS
                     GRVAKLKNGFIGRFGWKGQKARLSEFVLTACAVELGLNVPGEPQSGMPQRPEYVPQGL
                     DLTQRECNALIAFVASLPAPQERATAHRTEAEYLDSGRKLFASVGCAACHTEKLGDVE
                     GIYSDLLLHDMGQELSDSGNYAGFAPNATEEEELKGPVPPLAAAAAEPVDESKLIGAT
                     RQEWRTPPLWGLRDSAPYLHDGRADTIEQAIAKHGGEATRSVRQFIRLSADERLQLTL
                     FLRSLQAPIP"
     gene            6383..7825
                     /locus_tag="VFB80_15995"
     CDS             6383..7825
                     /locus_tag="VFB80_15995"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35332.1"
                     /translation="MTAITLLGSTRRPCSGPTRRETLRAGALSLLGGLFNVPSLLALE
                     ESPGKYLRPAKAKSVVLLYLQGGAPTQDMFDMKPHAAGGVGGEFKPIATSNPGIEVCE
                     HLPLTSRWMHTAAVVRSVYHNGGCHKNLPMYTGYDVNLPDEEFRDSDPPSMGSVCAYL
                     ERDVPKELPTYAYLPCPLGWGEVRKKAGPHGGFLGRRYDPFCTECTAFVDHPPDDIWK
                     PQIVRGEPRLTDIELKEGITLDRLAGRQRLVEQLDEQRRGLDDGPALGSLPRHKRLAF
                     ELLTSAKIREAFDISREEDKVRDRYGRTLFGSATLLARRLVERGVRFVNVSYDNFSKR
                     FEVSKAGWDTHQRNFPMLKETLLPTFDQTYSAFVADLHERGLLDETLVVTMGEMGRTP
                     KINADGGRDHWTFCYSVLLAGAGIRGGMIHGASDEQAAFIKDKPVHIRDICATIYHLL
                     GIDPEMPVRDRSGRPVPVAHGGQAVREILA"
     gene            7935..8129
                     /locus_tag="VFB80_16000"
     CDS             7935..8129
                     /locus_tag="VFB80_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35333.1"
                     /translation="MVRFSLRTLLIVLLVAPPLLAGVYFVGLSMSATVGGRYLLGLLV
                     WLLPIALFAAHFLPLLRSRS"
     gene            8126..8428
                     /locus_tag="VFB80_16005"
     CDS             8126..8428
                     /locus_tag="VFB80_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35334.1"
                     /translation="MKFSIRDLLLLTLVVALGAAWWVDRRQLERAALDSRHAQAVAER
                     HHDEYRRAYIELHRAVERAGLSITSAGGQTHVVDPKQAAATAPSAVSASAKPGGVE"
     gene            8462..8701
                     /locus_tag="VFB80_16010"
     CDS             8462..8701
                     /locus_tag="VFB80_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35335.1"
                     /translation="MFKFTIRDLLWLTFVVALAIGWWLHHRAWFEKFSATAHDSEIWH
                     ARTEELQVRVDLLAEQLRANRASTPAAPTLNQPGN"
     gene            complement(8920..9216)
                     /locus_tag="VFB80_16015"
     CDS             complement(8920..9216)
                     /locus_tag="VFB80_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35336.1"
                     /translation="MLKIASSGRAKMSSRAHELRSRAKVVSLVAANAGVSLRCWICTG
                     AGGAGGAVGWPLQRGPRQGSGIAGRWSVGRPLLWAAARESDASEWSIPAPVKIT"
     gene            9545..10588
                     /locus_tag="VFB80_16020"
     CDS             9545..10588
                     /locus_tag="VFB80_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35337.1"
                     /translation="MSRPRHAFTLVELLVVIAIIGILVALLLPAVQFARESARRTHCS
                     NNLKQIGVALHLYHDTLRVFPFGKGPSYPGAAGYARWSAHSLILPYMEQKPIWDSINF
                     NFAPATPGMGGVINFMPAYANANGMNVIPSKMYIPSFLCPSDGLNTPQDWPGQNNYCG
                     NQGGTFLCDRGDLPAGPSDTLPSETNRGMLYFLSGVSMGQVVDGTSNTLIFSEKLRGR
                     GSPDKRSDLFVIASQTTLDDTFNTCNATNPLTATPLTSKWGYSWVMGENCCTLYNHVA
                     PPNYPSCAGIPFPGTMTNMAMQVPPSSNHPNGVMACLTDASVKYVSENIDVLTWRALG
                     TRNGYEALDKDAF"
     gene            10610..11050
                     /locus_tag="VFB80_16025"
     CDS             10610..11050
                     /locus_tag="VFB80_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35338.1"
                     /translation="MTRHFEMHVLVFVAITVAGCGGRTRQDYVPKTAAAREALDKALV
                     AWRDGEPPGRIEGKPAVHVGDTHRRAGQKLAKYEIVGELPSDEGRLFAVRVQLENPTA
                     EEKINYLVVGIDPLWVFRQEDYQMVTHWEHKMPPAAAANPPQQE"
     gene            11171..12646
                     /locus_tag="VFB80_16030"
     CDS             11171..12646
                     /locus_tag="VFB80_16030"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZN35339.1"
                     /translation="MAATIKQTFGLAARVLWMRLRFVLAVAGLFAVIALWNQLGALWD
                     RAVVWLSGEALTERGVSPDTEYFCPMCPGVLAAWPEKCPVCKMPLVRRTRGESQLLPE
                     GVTARMQISPYRVQLAGIKTSPVGYLPLAHEFRTRGTLREKDLVAAPISPWDVPYAAA
                     GAPVELRSDRLPAGTSLAGTIKSVAAADGAGQSHELQIRVSDAPQQLASGASLDVIAR
                     VPLASIEPYRSQPRELPPLTDGAPRSYFVCPDHPGWIRAGGGKCPFDDRPLEERRLRD
                     DQRLEWSCRWHAGEHHSETGTSCPHCQRPCNSPRVVTYVPPGEVLVVPESAVIDTGSK
                     KIVFVETMPGMFDGVLVELGAKEGGYLPVLSGLSPGQRVASAGAFLLDAETRLNPSLA
                     ASYFGAGSAAGSGSSAVIAAASSAAGQTPDLLGALNLPPDDLLLARRQKVCPITRMAL
                     GAMGPLIRVEVEGEPVFLCCAACKSRVRQSGNGSTAEPIVP"
     gene            12643..16878
                     /locus_tag="VFB80_16035"
     CDS             12643..16878
                     /locus_tag="VFB80_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN35340.1"
                     /translation="MIDALIDWSVRRRWLVVALAAILAVLGAWAAWETPIDAIPDLSE
                     TQLIVYAQWPGHSPQEIEEQLTYPLSLHLRQLSDVKTLRGSSEFNYALLHLIFAEGTS
                     VDAARRAASERLATFADLPAGASVRLAPDGPATGQIFWYTLHGAGRDLGELRAIQDWQ
                     VRTPLAGVEGVAEVASVGGMPRELHVTVDPQKLSDAGISIAAVTAEIRRALESPAGQV
                     AHSPTAEYFVRTRVDRNSYRSSDSADDPADWNGLKSVLRDLVLPTAHGTVKLGDIADV
                     SLGPGPRRGVLEMDGNEVVGGVVLMRYGESPLDVAQRLKEKLIEIAPSLPDGVYVTVG
                     YDRSPLIRQAVGTVTSTLLEAIVVASLCVVLVLLHFRASLVIAVSLPLAVLSSFLVLW
                     GLRASGLATIETNIMSLAGLAISIGVLVDSSIVMAENVMHHLHRRFADQPVRGDVRQM
                     VIAACQQVGRPIFFSVLIMLLSFLPVFALGGLEGRMFRPLAVTKTLALLASALLAVTL
                     VPALCTLLVRGRIRSERDSPLVRGLMDAYLPLLKLALDRPAWLIWVVAVTFLVGFAPL
                     GLRLAGWPVLLLAALAIGVLAVVATARSRVAAALLSVSLVVVALTADRHMQPLASEFI
                     TPLDEGTAMDMPITVPRASVSQAADDLKARDMVLCRFPEVAMVMGKAGRADTPTDPAP
                     IDMIETMISFRPQEFWPRRKLSSPALSRSIERTLDELVARRLIEGPRPAQRAATIADS
                     AAAIAPKFDILMREVAWQQNREHQQALGGELAMAAATAALRQAQSAGALTGGNRGLAA
                     LLASKFPASLKARLAQLPTELEAAEAERWIGTYLQTEKYVAADRPLNLSLLSSLEAHY
                     RQAWRDHVRRLDLDLAQRASLLVARLALEDLLARLSVRDEKLNAELAQLHRFRLSRPL
                     ATAVTSHHGTASYAPPQIDPLPVLDGVQAELAPRLARHISLARANRRDLSGFGGEMDQ
                     ALQMPGWTNVWTMPIQNRVDMLATGVNTTVGIRVLGPKLDDVVSASQQVAEVLKQVRG
                     ASNVVADPVRGKGYLDIDVRPDAAAELGIPLGDVNAAIEAALGGTTAAQLSLGRERMP
                     VRVRFPSATGEDVESLKRLIVGQVSNLSKNEETSISPGQVENLSYGLCYLGQVANIAI
                     SEGPASIKSENGSLRNYVRLNVRDRDAEDFVREARQIVAQKVSLPAGVRLEWTGQFEH
                     QVRAKARLWLVAPLVLVVILGILYFTYRDLADALLVVPAVIGALAGGLLLQWLLGYTT
                     SVTVAIGYLACFGMAAATGIIMLVYLRDAVEQAGGIGQIGSLAELREAVIRGAAHRLR
                     PKLLTEATTILGLAPLLWATGIGSEVIRPMTAPVLGGILVADEVIDLLLPLMFYWVRK
                     RRWERMHQMEEPGREIETQNQFHSQKPSISVRL"
     gene            16875..>17230
                     /locus_tag="VFB80_16040"
     CDS             16875..>17230
                     /locus_tag="VFB80_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35341.1"
                     /translation="MNLRIALLFSVAVALAGCGGNSSTSYIPKGNDAQDALKLALDAW
                     KNGQTGEPAGKLPSGATVRGIDADWAAGEQLASYEIVKELPAEADAGPRKLIVKLTYE
                     GGNPIEATYYVVGIDPL"
BASE COUNT         2939 a         5521 c         5731 g         3039 t
ORIGIN      
        1 tagcgggact gatgcgcttc acgttaagca ctcctcatgt cgtcgaatca atggtgtccg
       61 ggcgtgacgt ttattcctgc cgcaggggca aggcgtgttt tgttttgttg acgtggcatt
      121 gagcgtgggt attagaagca aatgcagtgc cgcgagttct tcgctcgtcg gctcatggct
      181 ggacggacgc cttgaccagc atcagggcca gcacgaccac cgtgctgatg gcgatcggca
      241 cggcaaacgg gatcacgcgc cggccgcgcg gctggttctt cgccgccgtt tcccggtcct
      301 cgtccacgtt cagatagcgc cggcgcgtag cggatatgcc gcgcgtgcac atgccgccga
      361 ccatcacccc cgccgccatc accgcgccaa tgacggcact ggcgccgaag acgatgagaa
      421 tcgtcagcgg tcccagccac acacccagtg ccgccagcaa tttcacgtcc cccatcccgc
      481 cgccgccgag cagccacggt agcaagagca gcgagaagcc gacggcaaaa ccggccagcg
      541 cgcagagaat gccgctgccg gcaggcgcgg ccgcgtggaa caccagaccc gccaatgctg
      601 cggggaccgt gatccagttg gggatttttt tggtgcgcag gtccagggcc gcggcaatga
      661 tgacgaaaag caacaccaag gctgcctgaa gatgcaagaa cgtcacgcgg ggcctcacga
      721 agaggaggta ggcggccgat tcacccgccg gatcgattcg ccgggcgcga tcggccgcgg
      781 aagttgggtg cggtgggaag aatggcaacc agcgtgcccg aagtacgccg gttctcctgg
      841 cctcggccct tcctgattgg ctccacgacc gaccgccaaa tcagttgttt gtttctggcg
      901 ctgaggcacg aaggcgcgga ggaaccgagc agcaaaggtc tctaatttct actccgtgcc
      961 tctgcgtctc cgagcctctg tgtaaaaaga cgacaacctc gattttggcg aaacgcgtgg
     1021 cacttcgggc ggcgcggcca cggaataagt tccagcaatt cgctacgatt ccacgtcgtc
     1081 cacggccggc tggccgggga gcgtgttgcg agcgcaatcg acgaccgttc ccggatcgtc
     1141 ctcgaacacg ctctcgggaa tgcagtccac gccggcaaag ctgaagctct ggtcgaagtt
     1201 tagcaccgac gcggccacgt cggacagctc gtcgagaatg ccgtcgcggg cggcactcag
     1261 gccgccgacg atgccgaaga cgagcaagac cgcgacgagg caccattcga acgagagtgc
     1321 gccgtcgtct tcacgccaga gggccaggag ggtgcgtttc atcgtttgat cctgaactgc
     1381 cgctgcggaa cgctctgagg aattacgagc cgttgtcgct gagcgcggtt tgaccgcccg
     1441 gcccgagctc gcgtgcgcag tcggtataga ccatggcgtc gtcggtgaac gacatgccgg
     1501 gggcgaagat cggcggatcg acgatgtcga tctccgccag gctgtagctc tggtcgatgg
     1561 cgccggcggc ctgggcgacg tcccccagct cgtcgatgat ggcgtcgcgg gcggcggaca
     1621 ggccggccac gatgccaatc accagcagcg tgaggaccat cgtccattcg aaggagagga
     1681 tgccgtcctc ttcgcgccag agagctacca cggtgcgttt catcgcttga tccttaagtt
     1741 cggtttcgag ttcaatcttc aattcacaac ccggaactcg gaacacaaag ctttccgctt
     1801 gtgcaaagct cgggccgctt acgagccgtc atcgctgagc gcggcctggc cggcaagctc
     1861 gctgcggtcg caatcggcat aggtcgcccg gtcggtaaat gcggaatcgc tcgcgcctcc
     1921 cactgccaca ggagcgccgg gaccggcacc gtcggaatcg atcgacatgg ccagcggctg
     1981 gctgatgacg aatgacccgt ccagggcgag catggcctgg gcggcgtcac ccaactcgtc
     2041 gatcaccgcg tcgcgcgcgg cggacaggcc ggccacgacg ccgatgacca gcaaggtcag
     2101 gacgagagtc cactcgaacg acaggacgcc gtcctgctgc tgccagatct gccaggcgag
     2161 ccgtttcata ggggcgagct ttcgtcgcga aattcgggga gaaaagtgag tccacccggc
     2221 cggcgtgcgg ccgggtggac tctatcacca aagcactacg aagtgccgtc ggtcagcgca
     2281 tcctgaccgg gaggagcttc ctcgcgggcg cagtcgacga acagcgccgc gtcggtgaag
     2341 cccgagtcgc tggccgagct cggatcgtcg atgtgcacat cgacgtccag cgggaagctg
     2401 atcgtgtacg actggtccag ggccagcatg gcctgggcca cgtcacccag ctcgtcgatg
     2461 atcgcatcgc gagcgcccgt cagtccgctg acgatgccga tcgtcagcag ggtcacgagc
     2521 atgacccatt cgaaggagag gacgccgtcc tcttccttcc acatacgaga cagaaccttc
     2581 atccgaaact ccttgtacca gtactgcaga aaacacgtgt ggagcgggcc aacgttggcc
     2641 cctccgtcgg cgcgctttgt gcccgcgcac cgactttctc gtgtcggcag ccaatgcacc
     2701 tgacgtgcca cggccggcag tccttgcggc gcgcaagccg cctgcgccct gtaaccagtg
     2761 cccagcacgg gctttacggc tgtgcccgtg gaggattgtc tgcctggtca ttcccgccgc
     2821 gcgacagggg tctgaacaaa aggcgcatgc cccgcgtgac gccatgttca agcctcctag
     2881 cgccaggccc agggaagaaa cgcggccgcg gcagctccat cccagcgcgg aaatcgtctt
     2941 cgcgcatcat cttgccgatt ctgtcggcaa taacgttgga tggagttttg cgcggccgag
     3001 atcaaaaaaa cttggcgagc ctctcgtcgc gctacacgtc gcaaagacgg ccgtttcacg
     3061 tgctgaacat cgcgctcact aacccgaagc gcgaggcagg gagtttgata ggaacccgaa
     3121 gcgccagcga tagtgataat tccccgcgtt aatcggccgc cccttttgcg gttgaccatc
     3181 ccagcgccca ttggccagaa ttggccgctg ggtcgcggca tttcggcggg cagcggcatt
     3241 tggcgacaag gatcggccca tggcgcgcga cgacaatccg ttcggtccgc aacccaaacc
     3301 gaccgcgact agccagggga ttctcacttt gcccgccttc tggctggggc tgtcgcggga
     3361 tgtctcccaa gtctcgtatg ccgccacggg cctggggctg atggcggcga agtatgcggt
     3421 cgaggcgggg ctgatctacc actacacggg gcgttttttc agcccgctgg acttcctcaa
     3481 tccgctcctc agcatgcggg agcagttcct gcgggcgcca gcgccgccgt ggctgggctg
     3541 gtcgctcttt ttgtggtcgc tgccgtttgt gtggattgcc gtctccatga gcgtgcggcg
     3601 gtcggccaat gcgggagcga ccgcctggct ggggctgctg gtgctggtgc cgctggtcaa
     3661 ctacgtggtg atgctcgtgc tgtgcctgct ccccagccgg gccagtttca cctggcaggg
     3721 agagcggccc cgtccggtcg tcgatcatct ggtccgcagc gcgctactgg gaattctcgt
     3781 cagcgtcgcg atctcgatca gcatgatcct gctctgcgtg tacgtgctgg ccgattatgg
     3841 cgcggccttg tttctgggga cgccgatcct ggtgggcgcg gtcagctcgt tcatctacaa
     3901 ccgcccgtac ccacgcgacc tgggcagcag cctcgtcgtc gccgagctgg ccatctcgct
     3961 cagcggcgcg gccatcctgc tgttcgcctt cgaggggatc atctgtctgt tcatgctgct
     4021 gccgatcgcc ggcatcatgg ggctgctggg aggactgatc ggctacgcgg tggcctccct
     4081 gacgccgcac cggctggcgc tactgttgct gccgatcctg atattgccgc tggcggcagg
     4141 ggccgagcgg gtccaccggc caacgccgct gtacgaagtg atctccagcg tggagatcga
     4201 tgcgcccccc gagaaagtct ggccgcacgt ggtgggcttc tccgagctcc ccgcgccccc
     4261 cgcctggtac ttccgcctcg gcatcgccta tcccaagcgg gcgacgatcg aggggagcgg
     4321 cgtcggcgct gtgcggcggt gcgaattctc cacgggcccg ttcgtggagc cgatcacggt
     4381 atgggatcag ccgcggagac tggcgttcga cgtgcagtcg cagccgccac cgatgcacga
     4441 gctgagcccg taccgccacg tccacccgcc gcacctggac ggctacctcc gctgccgccg
     4501 cggcgagttc cggctgatcg agctgcccgg cggccgcacg cggctggaag ggagcacgtg
     4561 gtacgagttt gaaatgtatc cgcaggatta ctggacgctg tggtcggata cctgcatcca
     4621 ccagatccac cgccgcgtcc tggaccatat caaggtgctg gcggaggagg acatgaaagg
     4681 gattgagtga acgtcggctt tcgcggagcg aaagccgaca ctgaaaaatt gactgccgaa
     4741 caagcgatcc tttagactga ccaaaatctc gtgtgcctgt cagcccgtca ttctcgtctt
     4801 gggcggaggt gatctatgtt tgcattctgc ggcaatgggc ggagactctt ggtggcggcc
     4861 gctatgtcct gcgtttcgtt agcggtctgg ggcgcggcgg ccaatgccga ggaggccaaa
     4921 aaaatcgacg gccgcgagct gtttacacgc gaatggatcc ccggcgacgc gcggagccac
     4981 ggcggcgacg gcctggggcc ggtgttcaat gacacctcgt gcgtcggctg ccacaaccag
     5041 gggggtgtcg gcggcgcggg gcccaaggcc aagaacgccc agatcgtgac ggcattcagt
     5101 aaccgcgccg tcaatcaggg attcgaaccg gcgtccacgt cgctatttgg cgcgatcatc
     5161 accatgttcg gcgggcctcg tactgcatcg cccaggccac ccgcgaccga ggatgaagcg
     5221 gaccgcatcg ccaaggagcg cgacgacctg gccaagatcc accctgggtt ccgcacggcc
     5281 cgcagcgtgg tcctgcaccg agcttccacg gaatcggaat atgcggcgtt ccgcggcaag
     5341 ctcaacggca tggccaactt tgaggggatg gtcggaaaca ttgccatgga gggacgtatc
     5401 gtgccggcag gcgaatcggg tccgccactt tccgtcgagc agcagagtga aatcaaccgc
     5461 ctcaaggccg aagcccagtt cggcggccag caggcattta atggcggcgc tggcaacttc
     5521 acgttcctgg ttagcgagcg gaacacgatt cccctctttg gcagcgggaa gatcgacgtg
     5581 attccggaca acgtgctgat tgccgctgcc gagcagcggt tcgacgattt tcccgaagtg
     5641 agcggccgcg tggcaaaatt gaagaacggc ttcatcggcc ggttcggctg gaagggacag
     5701 aaggcgcggc tgagcgagtt cgtcctcacc gcctgcgcgg tcgagctggg gctgaacgtg
     5761 cccggcgagc cgcagtcggg aatgccgcag cgccccgagt acgtcccgca agggctcgac
     5821 ttgacgcaga gggaatgcaa cgcgctgatc gcgtttgtgg ccagtcttcc tgcgccgcag
     5881 gagcgtgcga cggcgcacag gacggaggcg gagtatctcg acagcggccg caagctgttt
     5941 gcctcggttg gctgcgccgc ctgccacacg gaaaagctcg gcgacgtgga agggatatac
     6001 agcgatttgc tgctgcacga catggggcag gagctgagcg actcggggaa ctacgccggc
     6061 tttgcgccga atgcgacgga agaagaagag ctgaaaggcc cggtgccgcc gctggcggcg
     6121 gcggcggctg agccggtgga cgagagcaag ctgatcgggg caacgcggca ggagtggcgg
     6181 acgccgccgc tgtggggcct gcgcgactcg gctccctacc tgcacgacgg ccgagctgac
     6241 accatcgaac aggcgatcgc caagcacggc ggcgaagcga cccgctcggt gcggcagttt
     6301 atccggctct cggcggacga gcggctgcaa ctgaccttgt tcctgcggtc gctacaggcg
     6361 cccatcccct agactcacaa tcatgaccgc catcacgctt ctcggcagca cgcgccgccc
     6421 ctgctccggt cccacgcgcc gcgagacgct cagggccggc gcgctgtcgc tgttgggggg
     6481 cctgttcaat gttccgtcgc tgctggcgct cgaagagtcc cccggcaaat acctccgccc
     6541 cgccaaggcc aagagcgtcg tgctcctcta tctccaggga ggagcgccga cgcaggacat
     6601 gttcgacatg aagccccacg cggcaggcgg cgtcggcggc gaattcaagc cgattgccac
     6661 gagtaacccg ggcatcgagg tctgcgagca tctgccgctg accagccgct ggatgcacac
     6721 ggcggcagtc gtccgcagcg tgtatcacaa cggcggctgc cacaagaacc tgccgatgta
     6781 caccggctac gacgtcaacc tgcccgacga ggaattccgc gacagcgatc cgcccagcat
     6841 gggctcggtg tgcgcgtacc tggagcggga cgtccccaaa gaattgccga cgtatgccta
     6901 tctcccctgc ccgctcggct ggggcgaggt ccgcaagaag gcggggccgc acggcgggtt
     6961 tctcggccgc cgctacgatc cgttctgcac cgagtgcacc gccttcgtgg accacccgcc
     7021 cgacgacatc tggaagccgc agatcgtccg cggcgagccg cggctgaccg acatcgagct
     7081 gaaggagggg atcacgctcg accggctggc cggccgccag cggctggtcg agcagctcga
     7141 cgagcagcgc cgcgggctcg acgacgggcc cgcgctgggg agcctgccgc ggcacaagcg
     7201 gctggccttt gagctgctca cgtcggcgaa aattcgcgag gcgttcgaca tcagccgcga
     7261 agaggacaag gtgcgcgacc gctatgggcg gacgctgttc ggctcggcca cgctcctggc
     7321 ccggcggctg gtggagcggg gcgtgcggtt tgtgaacgtc agctacgaca acttctccaa
     7381 gcggttcgag gtgagcaagg cgggctggga cacccaccag cggaattttc ccatgctcaa
     7441 ggagacgctg ttgccgacgt tcgaccagac ctactccgcg ttcgtcgccg acctgcacga
     7501 gcggggcctt ttggacgaga cgctggtggt gacgatgggg gaaatgggac ggacgccgaa
     7561 aatcaatgcc gacggcggcc gcgatcactg gacgttctgc tacagcgtgc tgctggcggg
     7621 ggccggaatc cgcggcggca tgatccacgg cgcgtcggac gagcaggccg cgttcatcaa
     7681 ggacaagccg gtccacatcc gcgacatttg cgcgacgatc taccacctgc tggggatcga
     7741 tccggaaatg ccggtgcggg accgcagcgg tcggccggtg ccggtggcgc acggcggcca
     7801 ggcggtgcgg gagatcttgg cctgagcggc tgaacgacta gcggggtcgg gagtcgattc
     7861 gggccgataa ctttgcaagt ggaaaaacgt tttccccgaa ttgactcccg acccctttgg
     7921 gctggcccac ccccatggtc cgcttctcac ttcgcacgct gctgattgtg ctgctggtgg
     7981 cgccgccgct gttggcgggt gtctacttcg tggggctttc gatgagcgcg accgtcggtg
     8041 ggaggtattt gttgggcttg ttggtttggc tgttgccaat tgcgctcttt gccgctcact
     8101 tccttccctt gctgcgcagc cgctcatgaa gttctccatc cgcgatttgc tgctgttgac
     8161 tctggttgta gccctagggg ccgcctggtg ggtggaccgg cggcaattgg agcgtgcggc
     8221 actcgattca cgtcatgctc aggccgtggc ggagcgccac cacgatgaat accgacgcgc
     8281 ctatatcgaa ctccaccgtg ccgtggagcg agcaggtctt tccatcacca gcgctggcgg
     8341 tcagacccat gtcgtcgatc ccaaacaagc cgccgctaca gctcccagcg cggttagcgc
     8401 ttctgccaag ccaggcggcg tcgagtaggg tttacaaagc attcgggctg taacattgac
     8461 catgttcaag ttcacgatcc gcgatttgct ctggctgacg ttcgtggtcg cgctcgcgat
     8521 tgggtggtgg cttcaccatc gcgcttggtt tgagaaattc tcggccacgg cacatgacag
     8581 cgaaatctgg cacgcgcgga cggaagagct gcaagtgcga gtcgatttgc tggcggagca
     8641 gttgcgagcc aatcgagcga gcacgccggc ggcaccgacc ctaaaccagc cagggaatta
     8701 ggcaggaaag gcaaaacgga ttcaccaccc acgcgacaat tcccttgcag cccgtggcgt
     8761 ggacgaatat ccttaaagga tgggggttta gggtcggttt cgtccgcgga tgacgtgcat
     8821 gtatgaacca tccgagcgcg cccggggctg ggctgcggca gtattacttg tgctgctgac
     8881 aggtctcgtg ccgcgcgctg cgctggccgc ctgcggtgac tatgtaatct tcacgggcgc
     8941 ggggatggac cattcgctgg cgtccgattc gcgagccgcg gcccagagca gcggtctgcc
     9001 aaccgaccat cgacccgcga tgccgctccc ctgccgcgga ccgcgctgca gcggccagcc
     9061 gaccgcaccg ccggcgcccc ccgcgccagt gcagatccag caccgcagcg aaacgcccgc
     9121 attggcggca acgagcgaaa cgaccttcgc ccgcgagcgc agctcatggg cccgcgaaga
     9181 catcttggcc cggccgctgg acgcgatctt gagcatcttt caccctccgc ggccctgcgc
     9241 ctgatcggcc gcttgagcct gcgcgaacca tcgcgccctg gcggcccgga tccggccaga
     9301 gagcacgccc ggcgcatgcg ccagcgtcta tttgcgccgg ctcccttggg gggcagttcg
     9361 attcgcaatg attgtagcgc ctacgcaggc gccccttacc cgcggaaatg cgatgcctcg
     9421 cgaaagcggg gtttccccag cggggtataa gccttacgga cgattggtta acaaggccac
     9481 tgccggcctt gacgcacgct ccccaggatt cgtttttatc ttttcatttt tcaagggtcc
     9541 gttcatgtcc cgtccacgcc acgctttcac gctggtcgag ctgctggtgg tcatcgccat
     9601 catcggcatc ttggtcgcac tactcttacc ggctgtgcaa tttgcgcggg agtcagcccg
     9661 ccgcacgcat tgcagcaaca acctcaagca gatcggggtc gccctgcatc tgtatcacga
     9721 cacgctgcgg gtcttcccct tcggcaaagg accgagctac cccggcgccg ccggctacgc
     9781 tcgctggtcc gcgcactccc tgatcctgcc ctacatggag cagaagccga tctgggacag
     9841 catcaatttc aacttcgccc ctgccacgcc cggcatgggg ggcgtgatca acttcatgcc
     9901 cgcctacgcc aacgccaacg gcatgaatgt gatccccagc aagatgtata tcccatcgtt
     9961 cctctgcccc tccgacggtc tcaatacgcc gcaggactgg cccgggcaga ataactactg
    10021 cggcaatcaa ggagggacct tcctctgcga ccgcggcgac ctgccggcgg ggccgagcga
    10081 caccctcccg tcggaaacca accgcggaat gctgtacttc ttgagcggcg tgtcgatggg
    10141 ccaggtcgtg gacgggacca gcaacaccct gatcttcagc gagaagctgc gcggccgcgg
    10201 cagtcccgac aagcggagcg acctgttcgt gatcgccagt cagacgacgc tggacgacac
    10261 gttcaacacc tgcaatgcga ccaacccgct gacggcgacg ccgctgacca gcaagtgggg
    10321 ttatagctgg gtgatgggtg aaaactgctg cacgctctac aaccacgtcg cgccgccgaa
    10381 ctatccatcg tgcgcgggga ttccgttccc ggggacgatg accaacatgg cgatgcaggt
    10441 gccccccagc agcaatcatc ccaacggcgt aatggcctgc ctgacggacg cgtccgtgaa
    10501 gtacgtcagc gagaacatcg acgtgctgac gtggcgggcc ctggggacgc ggaacggcta
    10561 tgaggcgctg gataaagatg cgttctagcc aatggtttgg gtcttttccg tgacgcgcca
    10621 tttcgaaatg cacgtgttgg tgtttgtcgc tatcaccgtg gccggctgtg gcggccgcac
    10681 gcgccaggac tacgtcccca agacggccgc ggcccgggaa gcgctcgata aggcgctggt
    10741 agcctggcga gacggcgagc cgcccgggcg gatcgaaggg aagccggcgg tccacgtcgg
    10801 ggacacgcac cgccgcgccg gccagaagct ggccaaatac gagatcgtcg gcgaacttcc
    10861 gtccgacgaa gggcggctgt ttgccgtccg cgtgcaactg gagaatccaa ccgccgaaga
    10921 aaagatcaac tatctggtcg tcggcatcga tccgctgtgg gtcttccgcc aggaggacta
    10981 ccagatggtg acgcactggg agcacaagat gccacctgct gcggcggcga acccgccgca
    11041 gcaggaatga ccaaatccca agcaccaatg accaatggtg cgagccaaac gcgggttcat
    11101 tggtcattgg gatttggtca ttggtcattc gtgcatgttt ggtgcttgag atttgagatt
    11161 ttgcccaccg atggccgcaa ccattaaaca aacttttggt ctggccgccc gcgtcctgtg
    11221 gatgcggctg cggttcgtgc tggccgtggc cggactgttt gccgtcatcg cgctgtggaa
    11281 ccagctcggc gcgctgtggg accgcgcggt ggtatggctg tcgggcgaag cgctgaccga
    11341 gcggggcgtg tcgcccgaca ccgagtattt ctgcccgatg tgcccgggcg tgttggccgc
    11401 ctggcccgag aaatgccccg tctgcaaaat gccgctggtg cggcggacca ggggtgagag
    11461 ccagctcttg ccggagggcg tcaccgcgcg gatgcagatt tccccctatc gggtccagct
    11521 tgccgggatc aagaccagcc ccgttggcta cttgccgctg gcgcacgagt ttcgcacacg
    11581 cggcactctg cgtgagaagg atctcgtcgc cgcgccgatc agcccctggg acgttccgta
    11641 tgccgcggcg ggcgcgccgg tcgagcttcg ttctgatcgt ctgccggcgg ggacgagcct
    11701 ggcgggcacg atcaaatcgg tcgccgcggc ggacggcgcg ggccagtcgc acgagctgca
    11761 gatccgcgtg agcgatgcgc cgcagcaact ggcttccggc gcgagcctcg acgtgatcgc
    11821 gcgcgtgccg ctggcgagca tcgagccgta ccgctcgcag ccgcgcgagc tgccgccgct
    11881 gaccgacgga gcaccgcggt cgtatttcgt ctgtcccgat catccgggct ggattcgcgc
    11941 gggcggcggc aaatgcccct tcgacgaccg cccgctggaa gaacgccgct tgcgcgacga
    12001 tcagcgattg gagtggtcct gccgctggca cgcaggcgag catcactccg agaccggaac
    12061 aagctgcccg cactgccagc ggccgtgcaa cagccccaga gtcgtcacgt acgttccgcc
    12121 gggcgaagtg ctcgtcgtcc ccgagtcggc agtgatcgac accggatcga aaaaaatcgt
    12181 ctttgtggaa acgatgccag ggatgttcga cggcgtgctg gtcgagctgg gggcgaagga
    12241 aggcggctat ttgccggtct tgtcgggatt gtcgccggga cagcgggtgg ccagtgccgg
    12301 cgcgtttctc ctggatgccg agacgcggct caatcccagc ctggcggcca gctacttcgg
    12361 ggcgggttcc gcggccggca gcggctcgtc ggccgtgatt gccgcggctt cgtcggcagc
    12421 aggccaaacg cccgatctgc tcggcgcgct gaacctgccg ccggacgacc tgcttttggc
    12481 acgccgccag aaggtctgcc cgatcacgcg catggccctg ggcgcgatgg ggccgctgat
    12541 tcgcgtcgaa gtggaagggg agccggtgtt tttgtgctgc gcggcatgca agtcccgcgt
    12601 gcgccaatcg ggcaatggct caaccgcgga gcccatagtg ccatgatcga cgcgctgatc
    12661 gactggtcgg tccgccgtcg ctggctggtc gtggcgctgg cggcaattct ggcggtgctc
    12721 ggcgcctggg ccgcctggga aacgccgatc gatgccattc ccgacctgtc ggaaacgcag
    12781 ctcatcgtct atgcccagtg gcccgggcac tcgccccagg aaatcgaaga gcaattgacg
    12841 tatccactct cgctccatct gcggcagcta tcggacgtga aaacgctccg cggctcaagc
    12901 gaattcaact acgcgctatt gcacctgatc ttcgcggaag ggacgagcgt cgatgcggcc
    12961 cgccgcgccg ccagcgagcg gctggccacg tttgccgatc ttcccgccgg ggccagcgtg
    13021 cggctggcac ccgacggtcc ggccacgggg cagatcttct ggtacacatt gcacggcgct
    13081 ggccgcgatc tcggtgagct gcgggcaatc caggactggc aggtgcgcac gccgctggcc
    13141 ggcgtcgaag gcgtggccga ggtggccagc gtcggcggaa tgccgcggga gctgcacgtg
    13201 acggtcgatc cgcaaaagct gagcgatgcg ggaatctcga tcgccgccgt gactgcggag
    13261 attcgccggg cgcttgagtc gccggcgggg caggtggccc attcgccgac ggcggagtat
    13321 tttgtccgca ctcgcgtaga ccggaattca taccgttcct cggattccgc tgatgaccca
    13381 gcagattgga acggattgaa gtccgttcta cgtgacctcg ttctccccac ggcccacggc
    13441 acggtgaagc tcggcgacat cgccgacgtt tccctcggcc ccggtccgcg ccgcggcgtg
    13501 ctggaaatgg atggcaatga agtggttggc ggcgtggtgc tgatgcgcta tggcgaaagc
    13561 ccgctcgacg tcgcgcagcg gctgaaggaa aagctgatcg agatcgcccc gtcgctgccc
    13621 gacggcgtct acgttacggt cggctacgac cgctcgccgc tgatccgcca ggcggtgggg
    13681 acggtgacca gcacgctgct ggaggcgatc gtcgtggcca gcctgtgcgt ggtgctagtc
    13741 ctgttgcact ttcgcgcgtc gctggtgatt gccgtctccc tgccgctggc ggtgctttcc
    13801 tcatttctgg tgctgtgggg gctgcgggcc agtggcctgg caaccattga gacgaacatc
    13861 atgtcgctgg ccgggctggc gatttccatc ggcgtgctcg tcgattcgtc gatcgtgatg
    13921 gcggaaaacg tgatgcacca tctgcaccgg cggttcgcgg atcagccggt ccgtggagac
    13981 gtacgccaga tggtgattgc cgcctgccag caggtcggcc gcccgatttt cttctccgtg
    14041 ctgatcatgc tactgtcgtt cctgccggtc ttcgctctgg gcgggctgga agggcgcatg
    14101 ttccgcccgc tggccgtgac caagaccctg gcgctgctgg cctcggcact tttggcggtg
    14161 acgctcgtgc cggcgctgtg cacgctgctg gtgcgggggc ggattcgcag cgagcgcgac
    14221 agcccgctgg tccgcggtct gatggacgca tacctgccgc tcctcaagct ggcgctcgat
    14281 cggccggcgt ggctgatatg ggtcgtcgcg gtcacgttcc tggtgggttt tgcgcccctg
    14341 gggctcagac tggccggatg gccggtgctg ctgttggcgg ctctagcgat cggggtgctg
    14401 gcggttgtcg ccaccgcccg cagccgcgtc gcggccgcgc tgctgtcggt cagtctcgtg
    14461 gttgtcgcgc tcacggccga ccgccacatg cagccgctgg ccagcgagtt catcacgccg
    14521 ctggacgaag ggacggcaat ggacatgccg atcaccgtgc cgcgggcgtc ggtgagccag
    14581 gcggccgacg acctgaaggc ccgcgacatg gtgctgtgcc gctttccgga agtggccatg
    14641 gtgatgggga aggcggggcg ggccgacacg ccgactgacc cggcgccgat cgacatgatc
    14701 gagacgatga tcagcttccg gccgcaggaa ttctggccgc ggagaaaact ttccagcccc
    14761 gccctttcgc gcagtatcga gcggacgctc gacgaacttg tcgcgcggcg gctcatagaa
    14821 gggccgcggc cggcacagcg ggcagcgacc atcgccgata gtgccgccgc gatcgcgccg
    14881 aagttcgaca tcctgatgcg cgaggtcgcc tggcagcaga accgcgagca tcagcaggcg
    14941 ctgggcggcg agctggccat ggcggcggcg accgcggcat tgcggcaggc gcagtcggcc
    15001 ggcgcgctaa ccggcggcaa tcgcggcctg gccgctttgc tggcgtcgaa atttccagcc
    15061 agtctcaagg cgcggctggc gcagcttcct accgagctgg aagcggccga agcggagcgc
    15121 tggatcggaa cttacttgca aacagaaaag tacgtggccg cggatcggcc cctgaatctg
    15181 tcgctgcttt cgtcgctgga ggcgcactat cggcaagcgt ggcgcgacca cgtgcggcgg
    15241 ctcgatctcg acctggcgca gcgggccagc ttgctcgtgg cgcggctggc actggaggac
    15301 ctgctggcgc ggctttccgt gcgtgatgaa aaactgaatg ctgagctggc gcagcttcac
    15361 cgctttcgcc tgtcgcggcc gctggctacc gcggtcacca gccatcacgg cacggccagc
    15421 tatgccccgc cgcaaattga tccgctgccg gtgctcgatg gcgtgcaggc ggagctagcg
    15481 ccgcgcctgg cgcgccacat ttcgctggcc cgcgccaacc gcagggatct gtccggcttc
    15541 ggtggcgaga tggaccaggc cctgcaaatg cccggctgga ccaacgtctg gaccatgccg
    15601 atccagaacc gtgtggacat gttggcgacc ggcgtcaaca cgaccgtcgg cattcgcgtt
    15661 ctggggccga agctggacga tgtcgtctcc gccagccagc aggtcgccga agtcctcaag
    15721 caagtacgcg gcgccagcaa cgttgtggcc gatccggtcc gcggcaaagg ttatctcgac
    15781 atcgacgtgc gccccgacgc ggcggccgag ctgggcattc cgctggggga cgtcaacgct
    15841 gcgattgaag cggcactcgg cggcacgacc gccgcgcagc tttcgctggg gcgcgagcga
    15901 atgccggtgc gagtgcggtt tccctcggcc accggcgagg atgtggaatc gctcaagcgc
    15961 ctcatcgtag gacaggtttc caacctgtcc aaaaacgaag aaacctccat ctcgccagga
    16021 caggttgaaa acctgtccta cgggctttgc tacctcggcc aggtggccaa catcgccatc
    16081 agcgaggggc cggccagcat caagagcgag aacggatcgc tgcgaaacta cgtgcggctc
    16141 aatgtccgcg atcgcgacgc cgaggatttc gtccgcgagg cgcggcaaat cgtggcccag
    16201 aaggtgtcgc tgccggcggg tgtgcggctg gagtggaccg gccagttcga gcaccaggtc
    16261 cgtgccaaag cgcggctgtg gctggtcgcg ccgctggtgc tcgtggtgat tctcggcatt
    16321 ttgtacttca cctaccgcga cctggcggat gcactactcg tggtgcccgc ggtcatcggc
    16381 gcgctggctg gcgggctgct actccagtgg ctgctgggat acacgacgtc ggtgacggtc
    16441 gccatcgggt acctggcctg cttcggcatg gcggcggcca ccggcatcat catgctcgtc
    16501 tacctccgcg acgcggtcga acaggccggc ggcatcgggc agattggctc gctggccgag
    16561 ctgcgggagg cggtgatccg cggcgcggcc caccggctgc gtcccaagct gctgaccgag
    16621 gccacgacga tcctcggcct ggcgccgctc ttgtgggcga cagggatcgg ctcggaagtg
    16681 atccgcccga tgacggcgcc ggtcctcggc ggcattctgg tggcggatga agtgatcgac
    16741 ctgctgctgc cgctgatgtt ttactgggtg cggaagcggc ggtgggagag gatgcatcag
    16801 atggaagagc cggggcgtga aatcgaaact caaaatcaat tccattctca aaaaccttct
    16861 atttcagtca gactatgaac cttcgcatcg ctctattgtt ttcggtcgcc gtggccctcg
    16921 ctggctgcgg cggcaattcg tccaccagct acattcccaa ggggaacgac gcccaggacg
    16981 cgctgaagct cgcgctcgac gcctggaaaa acggccagac gggcgaaccg gccggaaagc
    17041 ttccttcggg cgcgaccgtg cgcggcatcg atgccgactg ggccgctggc gagcagctcg
    17101 ccagctatga gatcgtcaaa gagctgcccg ccgaggccga cgccggaccg cggaagctga
    17161 tcgtcaagct cacctacgaa gggggcaacc cgatcgaggc gacatactat gtcgtgggca
    17221 tcgatccgct
//
LOCUS       DASRJB010000608         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2997928, whole genome shotgun sequence.
ACCESSION   DASRJB010000608 DASRJB010000000
VERSION     DASRJB010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997928"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFB80_16045"
     CDS             <1..466
                     /locus_tag="VFB80_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35342.1"
                     /translation="VAPMSNPYQSPLSEQFYNQARQSVEAARQEVINSVQAPALSLMI
                     VSGISIGVFLLAIPFDLLLLVIGGMAQADDSHIVTIVVRLIWSVAILAASGYCLYGAW
                     QMKTLNNYQHAFYASIVAMIPCLGPCCFLGIPFGAWAMTTLYKDEVRQRFQT"
     gene            complement(481..2376)
                     /locus_tag="VFB80_16050"
     CDS             complement(481..2376)
                     /locus_tag="VFB80_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN35343.1"
                     /translation="MTLLDPASCFRALVRPRAIAPDSGEVLPLEDLAAELLQPRSRRL
                     IEISGGPGAGKTTALRHLAATLPAAADAVFLDDAQAEEIGVYAGQRNVFFASRETERS
                     DIVLRLAPWGDDDLIEWLLAAAPQRCGDVMARLHAAADKEVLRGNPTLWHVALDQFLD
                     DASLSTIRGAVVAWLAKLLKLPKERRLAADFSLAILLRDPVEALRAFERLCPDHVPRD
                     RYRPLRHAFVQQLLAAQRLANLIRSGGSCPALHASLPLPLIEELALWVRSDPAVGEAL
                     RSIMKSDRSRDHAQAATILFAADPAWRPDERQVAHLSGGAFPGAQWAGLRLPSQASQS
                     SDLSETNLTSASLTQAVLDGASLRKSVLARARLDKASLALADAHQTDLSGANLTAANA
                     SYMLLSWANLQGALCDDATLTGVNLEGADLRGARFRGANLERVVLRSCQVEGTDFQGA
                     NLRGASLPSLVLREAELQNACFEKADLSHCDLEGVSLPSANFFHATLTGAYLTGSRMP
                     AADFRRASLCGAGLADIHWEGADLRGADLRGCTFHMGSSRSGLVGSPYPGHGSKTGFY
                     TDDYNDQDFKAPEEIRKANLCGADLRGAILDGVDFYLVDLRGALFDAAYAEHLTRCGA
                     ILYDRCA"
     gene            complement(2455..2793)
                     /locus_tag="VFB80_16055"
     CDS             complement(2455..2793)
                     /locus_tag="VFB80_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35344.1"
                     /translation="MARKVVNRKELRAEAEAAEALGKAGEAAAGADGTKKAKAPAEKK
                     APAKRKSRSKTAEPERRKLFWGVFNQSLKRVALFDHTQRKAAEAKAAELTSGGKNPHF
                     VMKVKETISQ"
     gene            complement(2911..3213)
                     /locus_tag="VFB80_16060"
     CDS             complement(2911..3213)
                     /locus_tag="VFB80_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN35345.1"
                     /translation="MELDDELCLCFHVTKRKVVNFIRIEKPKRPAQLSECFGAGTGCG
                     WCRPFLERLFEQAVLGGETSVDFPTPEEYAKLRGKYVRAGGGTPPPGAAPIPDDAE"
     gene            3343..3606
                     /locus_tag="VFB80_16065"
     CDS             3343..3606
                     /locus_tag="VFB80_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35346.1"
                     /translation="MSQPLSAPEILNREFLEMRCKILDLAAAFDRLGRADGTAADDPR
                     LARLREGLTIVLEEPHERAEHVQMLFSRAYDDGWREAFKVKPR"
     gene            3730..>4196
                     /locus_tag="VFB80_16070"
     CDS             3730..>4196
                     /locus_tag="VFB80_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35347.1"
                     /translation="MRIDAIPIWAIFALTICLVMAAIEFGYRLGQSRHQRSEDEKESP
                     VSAIAGAILGLAAFMLAFAFGLVAERHAAKRALVREEAVAIRTAWQRSDFLPEADRDE
                     AAALLRQYVDARVKFAQAGSLEPERVKGILSESARLQARLWNMAVANARKDMNS"
BASE COUNT          713 a         1375 c         1347 g          761 t
ORIGIN      
        1 agtagcacct atgtcgaacc cctatcagtc cccgctgtca gaacagtttt acaaccaggc
       61 gcggcagtcg gtcgaagcgg cgcggcagga agtgatcaat tcggtccaag cgcccgcgct
      121 ctcgctgatg atcgtgtcgg gcatcagcat cggcgtgttc ctgttggcaa ttcccttcga
      181 tctgctgctg ctggtgattg gcggcatggc acaagcggac gattcgcaca tcgtgacgat
      241 tgtggttcgc ctgatctgga gcgtggcgat cctcgccgcc agcggctatt gcctctacgg
      301 cgcgtggcag atgaaaacgc tcaacaacta ccagcacgcc ttttatgcgt cgatcgtggc
      361 catgatcccc tgcctcggcc cctgctgctt tctgggcata ccgttcggcg cgtgggccat
      421 gacaacgctc tacaaagacg aggtccgcca gcggttccag acctgaagcg cgccgcgaat
      481 ctacgcgcag cggtcgtaga ggatcgcgcc gcaccgcgtc agatgctcgg cataagccgc
      541 gtcgaacagt gcgccgcgga gatcgacgag atagaaatcc acgccgtcga gtatcgcccc
      601 gcggaggtcc gcaccgcaca ggttggcctt gcggatttcc tcgggggcct tgaagtcctg
      661 atcgttgtag tcgtcggtgt aaaaacccgt tttgctgccg tggcccggat aaggactgcc
      721 gaccaggccg ctgcggctgc tgcccatatg aaacgtacag ccgcgaagat cggcgccgcg
      781 aaggtcggct ccctcccagt ggatgtcggc cagcccggcc ccgcacaggc tggcgcggcg
      841 aaaatcggcc gcgggcatgc gcgacccggt gagatacgcg ccggtcagtg tcgcatgaaa
      901 gaagttggcg ctggggagcg agactccctc caggtcgcag tgcgaaaggt cggccttttc
      961 gaagcaggcg ttttgcagct ccgcctcgcg gaggacgaga ctgggcagac tggccccacg
     1021 cagattggcc ccctggaaat ctgtgccttc cacctggcag ctgcgcaaca ccactcgctc
     1081 gaggttcgcg ccgcgaaacc tggctccgcg caaatcggca ccttccaggt tcacgccggt
     1141 caatgttgcg tcgtcgcaca gagcgccctg caggttggcc caactgagaa gcatgtacga
     1201 cgcgttggcc gcagtcaggt tagcacccga cagatcggtt tgatgggcgt ccgccagcgc
     1261 cagcgaagcc ttgtcgagcc gcgccctggc cagcacggat ttacgcagcg acgcaccgtc
     1321 cagcactgcc tgagtgaggc tagccgacgt gagattcgtt tccgagaggt cgctcgattg
     1381 cgatgcctgg ctcggcagtc gcaggcccgc ccactgcgcc ccaggaaacg cgccgccgga
     1441 cagatgcgcg acttgcctct cgtcgggtcg ccaggcggga tcggcggcaa agaggatcgt
     1501 cgccgcctgg gcatgatcgc ggctgcggtc gctcttcatg atcgaccgca gcgcctcgcc
     1561 gacggctgga tcggaacgca cccacagcgc caactcctcg atgagcggca aggggagcga
     1621 cgcgtgcagc gcggggcaac ttcctcccga gcggatcagg ttggccaggc gctgcgcggc
     1681 caggagctgc tggacgaacg cgtgccgcag cggccggtag cgatcgcgtg gcacatggtc
     1741 ggggcacagc cgctcgaagg cgcgcagggc ctcaacagga tctcgcagca aaatggccag
     1801 cgagaagtcg gcggccagcc ggcgctcctt gggaagtttc agcagctttg ccagccaggc
     1861 cacgacagcc ccgcgaatcg tcgagagcga ggcgtcatcc aggaactggt cgagcgccac
     1921 gtgccacagc gtcgggttgc cgcggagcac ctctttgtcg gccgcggcgt ggaggcgggc
     1981 catcacgtcg ccgcaccttt gcggggccgc cgccagcagc cattcgatca ggtcgtcgtc
     2041 gccccatggg gcgagccgca agacgatgtc ggacctttcc gtctcgcggc tggcgaaaaa
     2101 cacgttccgc tgtccggcgt agacgccaat ctcctccgcc tgggcgtcgt cgagaaacac
     2161 ggcatccgcg gccgccggca gcgtggcggc cagatgccgc aaggccgttg ttttcccagc
     2221 gcccggtccg ccggagattt cgatcagccg gcggcttcgg ggctggagca gctcggcggc
     2281 cagatcctcc aggggcagaa cctcgcccga gtcgggagct atcgcccgcg gccgcacgag
     2341 ggcccgaaaa cacgaggctg gatccagcag cgtcatgact ggtcacccgg cgtaagccgc
     2401 tcctgtcatg accttcaatg taagcgacgc agcgggcccg gcgtaccggc cggcctattg
     2461 cgagatcgtt tccttcacct tcatcacgaa gtgcggattc ttgccgccgg aggtcagctc
     2521 ggccgctttg gcttccgcgg cctttcgctg cgtgtgatcg aagagggcca ctcgcttcag
     2581 cgactgattg aacacgcccc agaagagctt gcggcgttcc ggctcggcgg tcttgctgcg
     2641 gctcttgcgt ttggccggtg ctttcttttc ggccggggcc ttggccttct ttgtgccgtc
     2701 cgctccggcc gcagcttcgc cggcttttcc gagggcctcc gccgcttcgg cctcagcacg
     2761 taactctttg cgattgacaa ctttgcgcgc catgttcgtt caagatcgag attgcgtagt
     2821 gataccgtgc gaggaattgc cggcggtcgc cacggggcgg tatcttaacg gattcttttc
     2881 cgccctgcta gttgccgagc gcttagcgaa ctattcggcg tcatccggaa tcggggcagc
     2941 cccgggcggc ggagtgccgc caccggcgcg gacatatttg ccgcgcaact tggcatattc
     3001 ctcgggcgtc ggaaaatcga cgctcgtttc gccgcccagc acggcctgct cgaacagacg
     3061 ctccagaaac ggccggcacc agccgcagcc cgtccccgcg ccaaagcact cgctgagctg
     3121 cgccggccgc ttgggcttct cgatgcggat gaagttaacc accttccgct ttgttacgtg
     3181 aaagcagagg cagagctcgt cgtcgagttc cataacggta ttttggccca tcaggcgtct
     3241 gccggcgagt ggcagctgcc tcgccgcgcg cttgattttc gctggccgcg gtaggaaact
     3301 agcagttgag ccgttcgccg ctccattcgt ccaagtacac caatgtccca gcctctctcc
     3361 gcgcccgaaa tcctgaatcg cgaattcttg gaaatgcggt gcaagattct cgacctcgcc
     3421 gccgcattcg accgcctggg ccgcgccgat ggcacggccg ccgacgatcc gcgcctggct
     3481 cgcctccgcg aaggactgac gatcgtcctc gaagagccgc acgagcgcgc cgagcacgtg
     3541 cagatgctct tttcgcgcgc ctacgacgac ggctggcgcg aagcgttcaa ggtaaagccg
     3601 cggtaggact tcccatttgc tgggcgcgaa gttctgcttc tccaaaacat cccaggccaa
     3661 ccggctctga tgccggtcac gcaatcgagt gcgtgattga cgcacgagac tttgcgaggt
     3721 ggatcaccga tgcgcatcga tgccattccc atttgggcga tcttcgccct gacgatctgc
     3781 ctcgtgatgg ctgcgatcga atttggttat cggctcgggc aatccaggca ccagcggtcc
     3841 gaggatgaaa aagaatcgcc ggtttcggcc atcgccggcg ccatcctggg gctggcggcg
     3901 ttcatgttgg cgttcgcgtt cggactcgtg gcggagcgcc atgccgccaa gcgggcgctg
     3961 gtgcgggagg aagccgttgc catccgtacc gcctggcagc ggtcagactt tctgccggaa
     4021 gcggatcgcg atgaagccgc cgcgctgctg cggcaatacg tcgatgcgcg cgtgaagttc
     4081 gcccaggcgg gcagcctcga accggagcgg gtgaagggca tcctgtccga gtccgcgcgg
     4141 ctgcaagccc gcctgtggaa catggccgtc gccaacgcgc ggaaagacat gaactc
//
LOCUS       DASRJB010000609         9039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3278471, whole genome shotgun sequence.
ACCESSION   DASRJB010000609 DASRJB010000000
VERSION     DASRJB010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3278471"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..4834
                     /locus_tag="VFB80_16075"
     CDS             32..4834
                     /locus_tag="VFB80_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HZN35348.1"
                     /translation="MNQDGDAANGETPDDQYTAPIVIGDVHVATASDVPVQLSMFSTS
                     RSFIPFNQDLSIADLDVRVDINYPDAGLLALTLVSPGGVRIPLVGVMGFLGPNFANTV
                     FDDEAAVPIASGSPPFTGSFRPSSPLSVLDGTSALGTWTLEVGDNWFHSGTLNSWSLA
                     IIANPPRLTISDVALSEGDSGTTDALFTVSLSNVIGEMVTVDFATASGSATAGSDYLP
                     RSGTLTFAPGELTKMIAVTIFGDMAEEPSEDFFVNLSNVANATLADAQGVGLIRNDES
                     RLTITDVTLAEGSSGSTSAVFTVSLAAPTSQTISVSYATASGTAQSGSDYTAASGTLT
                     FLPGETTKTIAVAVAGDSLNELDETFTVNLTNPVNAVLTDSQGVGTITNDDPLPALSV
                     NDVKITEGNSGTKTLSFGVSLSAASGRTVTVSYATLAGTATPGSDFTAASSTLTFNPG
                     QTNKTIGIVINGDATPESDETLLLRLDGASGAILLDSEGLGTIQNDDTSLSIADTTIA
                     EGDSGVGTVTFTVSLSTAVGFEVRVAYATANGTASGGSDYAAASGTLVFSPGQSSQTI
                     GVLVLNDRLNEVNETLLVNLSNPIGAVLADSQAAATLLDNDPLPSLSIAGGTISEGNT
                     GTKNLNFTASLSAASGRSVTVEYATLAGTAAAGTDFVPRSGTLTFLPGSVSQTISVVV
                     SGDTAAEADETLSLVLSNPANADLEASAAEGTIQDDDNLSISDTTMVEGDGGLFEAVI
                     TLSLAIPLGSEARVDYATSNGSAAAGADYVAASGSVIFAPGQTSQSIVVPVLGDRADE
                     LDETVLVNFTNPVNLVLADTQAVVTIADDDEQPLIRVADVTVVEGNSGTRNLAFVLTL
                     SAASNRTVTVQYTTADGTATAGSDYTAKSGTLTFLPGSAAQTINVTVSGDTAAEPSET
                     VLLNLSAPTNGTLDDDQAVGTIAGDDPLPLVAVNDVKVTEGQSGTKSLTFNVTLSSAS
                     TSAVSVDYAALGGTAMPGSDFTAAAGTLTFSPGQTSKLVSVAILSDTLSEPDENFTFN
                     LSNPSGAALLDGEGLGTIQNDDTTIGISDASVVEGDTGQAELTFTATLSAAVGFEVAV
                     HFTTANSGAIAGTDFVGDSGTLLFAPGQTSRTVTVLVLADLRDEADETLFVNLTTPAN
                     AVLADSQGVGTIVDDDAAPALSIADATLSEGHTGTKNLNFTVSLSAASGRTISVQYAT
                     SSGSAAAGSDYQPRSGTFTLLAGWTSGTITVPIVGETLAELDESLLVTLTSPSGASLA
                     DSQAVGTIQDDDNLSIGDITVVEGDGGAATALFTITLGVALPHEVRLDYATANGSATA
                     GSDYVATSGTVVMAPGQTSQSIAVPILGDRWDEADETILLNLLRPVGVILADTQVAAT
                     IADDDPLPRLSITDATIAEGNAGTRNLTFTVTLSEASGRNVSVQYATADSSATAGSDY
                     ASKTGTLTFLAGWTSQNITVTVSGDTAIEPDERLLVNLSGVTNGVIVDSQGEGLLLND
                     DTSGIAWLRSQPAAASGSSRSGNEGQGTEGAQQPQSSRDPAGPRPAAGQNLRPDVRWP
                     PELIDAVMRELSSRSKRSGDATPRPGPNRGELPEWDDSWKDLLPGWLR"
     gene            complement(4997..6760)
                     /locus_tag="VFB80_16080"
     CDS             complement(4997..6760)
                     /locus_tag="VFB80_16080"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN35349.1"
                     /translation="MSSPAQPDAPFTAPHPGSSEMPQWTTGELPAPPRLGWNLWALVG
                     PGIVMSASAIGGGEWLLGPTVTARYGGAMLWLATLSIVFQALYNVEISRYTLYCGEPI
                     FSGKFRTLPGPLFWIGLYLLFDFSTIFPYLAANAATPIQVILLGGEIPKPDQVAAHWW
                     LAKITSTVIFLLGMIPLVFGGKIFDSLRVVMSAKLAIVFSFLLVLAVLYSHWSTWVEI
                     GSGFFKFGNVPVRHGEDWNGNGLLDPGEDWDGDGRLDVVEEVSADGKLIDADGDGIRD
                     GDNAQNVFLSLARGEWPKIDFGLVAFITAMAAIAGNGGLSNTPMSNYIRDQGWGMGQH
                     VGAIPSFVGGRGIGLSHVGCVFEVTEQVLPRWRQWYWKVVREQLLIWMPACFVGLALP
                     SMMSVEFLQRGTQAGDWNGAVMTAKGVQEHVAQPAAGTLAYESGLSNVLYGRAWGNLF
                     WSLTLLCGFLVLATTLVSTTDGIIRRWVDAFWTSSRRLREVHPEAIRHVYFGVLLAYV
                     AFGMVALWLNKPAALITWATLGFNLALGFSCWHTLAINLLLLPRELRPGWFMRISLIL
                     GGLFFAALFAVTVLEKLGAFK"
     gene            6934..7905
                     /locus_tag="VFB80_16085"
     CDS             6934..7905
                     /locus_tag="VFB80_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZN35350.1"
                     /translation="MPSLRRFINQLSEREPIDQVFLVADKQMRANRNGNLYLQLRLTD
                     KTGALTGMLWNATDQHFDSVQTGNYLRVQGTSQVHNGQMQIIVTRIEPADARQVDDED
                     FITVTAAEIDKLAARLAEMLRAVKNVHLRNLAECFLSDDEFMGKFTAAPAGIKNHHAY
                     RGGLLAHVVSLMDACRAITPLYPDLDGDLLVLGAFLHDAGKIDELTYDREFGYSDEGQ
                     LIGHVVMIISTLEDKVAESERLSAEKFPRELLLRLKHLIVSHHGEYQFGSPKLPMTLE
                     AIALHLLDNLDAKLYSVGQILKEDVGSDSTWTSYNQALGRKFFKGGK"
     gene            complement(7947..8426)
                     /locus_tag="VFB80_16090"
     CDS             complement(7947..8426)
                     /locus_tag="VFB80_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35351.1"
                     /translation="MDADFLVRRAELAGNSADGLMLRSRPGVATADCTDVTDEGRHFP
                     DIRASSEIRGFPAVRRELRPFEELPAEGLAVVRASATADCTDVTDEGRHFPDIRAISE
                     IRGFPAVRRELRPLEEPPAEGLADANFAICDGQFALCIRSVKCRAGLRGPGKLQIAN"
     gene            8501..8875
                     /locus_tag="VFB80_16095"
     CDS             8501..8875
                     /locus_tag="VFB80_16095"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZN35352.1"
                     /translation="MDAIAPPTPRPDVKTQAAIREVLSFLPPDVVQELNSGQIDLRDA
                     AFLEGLADHISRLAVASPTTGQKALGKFIKLKKLIARSLRDSDPGVRVSSTLHADQRI
                     GRNAKCPCGSGRKFKQCCWHRL"
BASE COUNT         1714 a         3149 c         2802 g         1374 t
ORIGIN      
        1 cccggcgatt ctcgacggcg ccggcaaccc catgaatcag gacggcgacg ccgccaatgg
       61 cgagacgccg gacgaccagt acaccgcccc gatcgtcatc ggcgacgtgc acgtggcgac
      121 ggccagcgac gtgccggtcc agctgagcat gttctcgacc tcgcgctcgt tcatcccgtt
      181 caaccaggac ctgagcatcg ccgatctcga cgtgcgggtg gacatcaact atccggacgc
      241 ggggcttttg gcgctgacgc tcgtttcgcc cgggggcgtg cggattccgc tggtcggcgt
      301 gatgggcttc ctcgggccga atttcgcgaa caccgtgttc gacgacgaag ccgccgtgcc
      361 gatcgccagc ggctcgccgc cgtttacggg ttcgttccgg cccagctcgc cgctctcggt
      421 gctggacgga accagcgccc tgggcacctg gacgctggag gtcggcgaca actggttcca
      481 ttccggcacg ctcaatagct ggtcgctggc gatcatcgcc aacccgccgc ggctgacgat
      541 tagcgacgtg gccctgtcag aaggagactc gggcacgacg gacgccctgt ttacggtttc
      601 gctgtccaat gtgatcggcg agatggtcac ggtcgatttc gcgaccgcca gcggctcggc
      661 gacggcgggc agcgactatc tgcccagaag cggcacgctc acgttcgctc ccggcgagct
      721 gaccaagatg atcgcggtca cgatcttcgg cgacatggcc gaagagccca gcgaggactt
      781 cttcgtcaat ctgtcgaacg ttgccaacgc cacgctggcc gacgcgcagg gcgtgggact
      841 gatccggaac gacgaatcgc gcctgaccat caccgatgtg acgctcgccg aaggcagcag
      901 cggctcgacc agcgccgtct tcaccgtcag cctggccgcg cccaccagcc agacaatctc
      961 cgtcagctat gccacggcca gcgggaccgc ccagagcggc agcgactaca ccgctgccag
     1021 cggcacgctg accttcctgc ccggtgaaac gaccaagacg attgcggtgg cggtcgccgg
     1081 cgacagcctg aacgaattgg acgaaacctt cacggtcaac ctcaccaacc cggtaaacgc
     1141 cgtccttacg gacagccagg gtgtgggcac gatcaccaac gacgatccgc tgccggcttt
     1201 gtcggtcaat gacgtcaaaa tcaccgaagg caactccgga acgaagacgc tcagctttgg
     1261 cgtcagcctg tcggccgcca gcggccgcac cgtgaccgtt tcgtacgcca cgctggccgg
     1321 cacggccacg cccggcagcg acttcaccgc cgccagcagc acgctcacat tcaaccccgg
     1381 ccagacgaac aagacgatcg gcatcgttat caatggcgat gcaactcctg agtcggacga
     1441 gacgctgctg ctgcgcctgg acggcgcgtc aggcgcgatc ctgctcgaca gcgaagggct
     1501 cggcacgatc cagaacgacg acaccagcct gagcatcgcc gacacaacga tcgccgaagg
     1561 agattccggc gtcggcaccg tcacgttcac ggtcagcctc tcgaccgcgg tcggctttga
     1621 ggtgcgcgtc gcctacgcca ccgccaacgg cacggcttcg ggggggagcg actatgcagc
     1681 cgccagcggc acgctcgtct tcagtccggg ccagtcgagc cagacgatcg gcgtgctcgt
     1741 cctcaacgac cggctcaatg aagtgaacga gacgctgctg gtcaatctgt cgaatccaat
     1801 cggagcggtg ctggccgaca gccaggccgc ggccacgctg ctcgacaacg atccgctgcc
     1861 gagcctctcg attgccggcg ggacaatcag cgagggcaac acgggaacca aaaatctcaa
     1921 ttttaccgcc agcctctcgg cggccagcgg gcgatcggtc acggtcgagt acgccacgct
     1981 ggccggcacc gctgcggccg gcacggactt tgtccccagg agcggcacgc tgacgttcct
     2041 gcccggctcg gtctcgcaaa ccatcagcgt tgtggtcagc ggcgacactg ccgccgaggc
     2101 ggacgagacg ctttcgctcg tgctcagcaa tccggcgaac gccgatctgg aggccagcgc
     2161 cgccgagggc acgatccagg acgacgacaa cctgtcgatc tcggacacga ctatggtcga
     2221 aggggacggc ggcctgttcg aggctgtcat cacgctcagc ctggcgattc cgctcggttc
     2281 ggaggcccgc gtggactatg ccacgtccaa cggctcggcg gccgccggcg cggactacgt
     2341 tgccgccagc ggctccgtaa tctttgcccc gggccagact tcgcaatcga tcgtggtccc
     2401 ggtcctgggc gaccgcgccg atgagctgga cgagacggtc ctggtgaact tcaccaatcc
     2461 ggtcaatctc gtgctcgcgg acacgcaggc ggtcgtgacg atcgccgacg acgacgagca
     2521 gccgctgatt cgcgtggccg acgtgaccgt ggtcgaagga aactccggta cgcggaatct
     2581 cgctttcgtc ctcacgctgt cggctgccag caaccggacg gtgaccgtgc aatacacaac
     2641 ggccgacggc accgccaccg ccggcagcga ctacacggcc aagagcggta cgctgacgtt
     2701 ccttcccggg tcggcggcgc aaacgatcaa cgtcacggtc agcggcgata cggccgccga
     2761 accgagcgaa accgtgctgc tcaacctgtc tgctccgacc aacggcacgc tcgacgacga
     2821 ccaggcggtg ggcacaatcg ccggcgacga tccgctgccc ctcgtagccg tcaacgatgt
     2881 gaaagtcacg gaaggtcaga gcggaaccaa gtcgctgacg ttcaacgtca cactctcctc
     2941 cgcgagcacg tcggccgtca gcgtcgatta cgcggcgctc ggcggcaccg ccatgcccgg
     3001 ctcggacttc actgccgccg ccggtacgct gaccttcagc cccggccaga ccagcaagct
     3061 cgtctccgtg gcaatcctga gcgacacgct gagcgagccg gacgagaact tcaccttcaa
     3121 tctgagcaat cccagcggcg cagccctgct cgacggcgag gggctgggca cgatccagaa
     3181 cgacgacacg acgatcggca tcagcgacgc gagcgtcgtc gagggggaca ctggacaggc
     3241 cgagttgacc ttcacggcga cgctctcggc cgccgttggc ttcgaagtgg ccgtccattt
     3301 caccaccgcc aacagcggcg cgatcgccgg cacggacttc gtcggagaca gcggcacgct
     3361 gctcttcgcc cctggccaga cctcccgcac ggtgaccgtg ctcgtgctgg ccgacctgcg
     3421 cgatgaagcc gacgagacgc tgtttgtgaa tctcacgacg ccggcaaacg ccgtgctggc
     3481 cgacagccag ggcgtcggca cgatcgtcga tgacgacgcg gcgccagccc tctccatcgc
     3541 cgacgccacg ctctccgagg gccacacggg caccaaaaac ctgaacttca cggtcagcct
     3601 ctccgctgcc agcggccgca cgatcagcgt gcaatatgcc acttccagcg gctcggccgc
     3661 cgcgggcagc gactatcagc cgcgcagcgg gacctttacg ctattggccg gttggacctc
     3721 cggaacgatc accgtgccga tcgtcggcga aacgctcgcc gagttggacg agtcgctgct
     3781 ggtcacgcta accagtccca gcggcgcgtc gctcgccgac agccaggccg tcggcacgat
     3841 ccaggacgac gacaacctct cgatcggcga catcacggtc gtcgaggggg acggcggcgc
     3901 agccaccgcc ctcttcacca tcacgttggg cgtcgccctg ccgcacgaag tgcggctgga
     3961 ctatgcgacc gccaacggct cggcaacggc cggcagcgac tatgtcgcga cgagcggcac
     4021 ggtggtcatg gctcccggac aaaccagcca gtcgatcgcc gttccgatcc tcggcgatcg
     4081 ctgggacgag gccgacgaga cgattctgct caacctgctg cggcccgtgg gcgtgatcct
     4141 cgccgacacg caggtcgccg ccacgatcgc cgacgacgat ccgctgccgc ggctgtcgat
     4201 caccgacgcc accatcgcgg aaggcaacgc cggcacgcgc aacctgacat tcaccgtcac
     4261 gctctcggaa gccagcgggc ggaacgtcag cgtccagtac gccaccgccg atagctcggc
     4321 gaccgccggc agcgactacg cgtccaagac cggcacgctt accttcctgg ccggctggac
     4381 ctcgcagaac atcacggtca cggtgagcgg cgacacggcg atcgagcccg acgaacggct
     4441 gctcgttaac ctgtccggcg tgaccaacgg cgtcatcgtc gattcgcaag gcgaagggct
     4501 gctgctcaac gacgacacca gcggaatcgc ctggctccgc tcgcagcccg ccgccgcgag
     4561 cggttccagc cggtccggca acgagggaca ggggaccgag ggcgcccagc agccgcaatc
     4621 gtcgcgcgac cccgccggcc cgcggccagc cgccggccag aacctacggc cggacgtccg
     4681 ctggcctccg gagctgatcg atgccgtgat gcgcgagctc tccagtcggt ccaagcgttc
     4741 aggcgacgct accccgcggc ccgggcccaa tcgcggcgaa ctcccggagt gggacgattc
     4801 ctggaaggac cttctgcccg gctggctgcg gtaaacttgc ttccgactcc agcgactcgc
     4861 gagcgacctg gcgacttagg gatctcgttc ccaagctccg cttgggaacg cacggctgga
     4921 agctctgctt gccatgccgc gaagcggagc ttcgctaact tgcgtgccca agcggagctt
     4981 gggcacgaga aaaacactac ttaaacgccc cgagcttctc cagcacggtc accgcgaaca
     5041 gagcggcgaa gaagaggccg ccgaggatca ggctgatccg catgaaccag ccggggcgca
     5101 gctcgcgcgg caagaggagc aggttgatcg ccagcgtgtg ccagcagctg aagccgaggg
     5161 ccaggttgaa gcccagcgtc gcccaggtga tgagcgccgc cggcttgttc agccagaggg
     5221 ccaccatgcc gaacgccacg taggccagga gcacgccgaa atagacgtgg cggatcgctt
     5281 ccgggtggac ttcgcgcagc cgccggctgc tggtccagaa ggcatcgacc cagcggcgga
     5341 tgatgccgtc ggtggtcgag acgagcgtcg tcgccagaac caggaagccg cagaggagcg
     5401 tcagcgacca gaacaggttg ccccaggccc ggccgtagag cacgttcgag agtccggact
     5461 cgtaggccag cgtgcccgcc gccggctgag cgacgtgctc ctgcacgcct ttagccgtca
     5521 tcacggcccc gttccagtcg ccggcctgcg tgccgcgctg caggaactcg accgacatca
     5581 tgctgggcaa ggccaggccg acgaagcagg cgggcatcca gatcagcagc tgctcgcgga
     5641 cgaccttcca ataccactgc cgccagcgag gcagcacctg ctcggtgact tcgaacacgc
     5701 agccgacgtg cgacaggccg atgccgcgtc cgccgacgaa gctggggatc gcgccgacgt
     5761 gctggcccat gccccaaccc tgatcgcgga tgtagttgct catcggcgtg ttggagaggc
     5821 cgccgttgcc ggcgatcgcc gccatcgccg taatgaacgc caccaggccg aagtcgatct
     5881 tcggccactc gccgcgggcc agcgacagga acacgttctg ggcgttgtcg ccgtcgcgga
     5941 taccgtctcc gtcggcgtcg atcagcttgc cgtccgcgga gacttcttcg acaacgtcca
     6001 ggcgcccgtc gccgtcccaa tcctcgcccg gatcgagcag gccgttgccg ttccagtctt
     6061 cgccgtgccg cacgggcacg ttgccgaact tgaagaaccc gctgccgatc tcgacccagg
     6121 tgctccagtg cgaatagagc accgccagca ccagcaggaa gctgaacacg attgccagct
     6181 tggcgctcat cacgacccgc agcgaatcga aaatcttgcc gccgaagacg agcgggatca
     6241 tgcccagcag gaaaatcacc gtcgaggtga tcttggccag ccaccagtgg gccgcgacct
     6301 ggtcgggctt ggggatttcg ccgccgagca ggatcacctg gatcggcgtc gccgcgttcg
     6361 ccgcgaggta cggaaagatc gtgctgaaat cgaacaacag gtagaggcca atccagaaca
     6421 gcgggccggg gagcgtgcgg aacttgccgc taaaaatcgg ctcgccgcag tacagcgtgt
     6481 agcggctgat ctcgacgtta tagagcgcct gaaagacgat gctcagcgtc gccagccaga
     6541 gcatcgcccc gccgtagcgg gcggtgaccg tcggccccag cagccactcg ccgccgccga
     6601 tcgccgacgc gctcatcacg atccccggtc cgaccagggc ccacaggttc cagcccagcc
     6661 gcggcggcgc gggcaattcg cccgtcgtcc actgcggcat ttcggaggag cctgggtggg
     6721 gcgcggtgaa gggagcgtcg ggctgggcgg gagaggacac ggcgcggcgg gggtcgggag
     6781 tcgggagtca ggagacagag tatgtcgttc atgcctgcgg ttcacaagcc gcgcgctcgg
     6841 cgtgctattc gctcgccgca ttcccccata gaatcggtct cgcgcgtgtt cgcggcaccg
     6901 actcccgacc cccgacgccc gacccccgac tccatgcctt ccctccgccg gtttatcaac
     6961 cagctcagcg aacgcgagcc gatcgaccag gtcttcctgg tcgccgacaa gcagatgcgg
     7021 gccaaccgca acggcaacct ctatctgcag ctgcggctca cggacaagac cggcgcgctg
     7081 acgggcatgc tctggaacgc caccgaccag catttcgatt cggtccagac gggcaactat
     7141 ctgcgggtgc aaggcacgag ccaggtccac aacgggcaga tgcagatcat cgtcacccgc
     7201 atcgagccgg ccgacgcccg gcaagtggac gacgaggact tcatcaccgt cactgcggcc
     7261 gagatcgaca agctggccgc gcggctggcg gaaatgctgc gggcagtaaa gaacgtccac
     7321 ctgcgaaacc tggccgagtg cttcctcagc gacgacgaat tcatgggcaa gttcacggcc
     7381 gctccggcgg ggatcaagaa tcaccacgcc taccgcggcg gcctcctcgc gcacgtcgtc
     7441 agcctgatgg acgcctgccg ggcgatcact cccctctatc ccgacctcga cggcgacctg
     7501 ctcgtgctgg gggcctttct gcacgacgcc ggcaagatcg acgagctgac ttacgaccgc
     7561 gagttcggct atagcgacga agggcagctc atcggccacg tcgtcatgat catcagtacg
     7621 ctggaagaca aagtcgcgga gtccgagcgg ctctccgcgg aaaaattccc ccgcgagctc
     7681 ttgctgcgac tcaagcacct gatcgtcagc caccacggcg aataccagtt cggcagcccc
     7741 aagctgccga tgacgctgga ggcgatcgcc ctgcacctgc tcgacaacct cgacgccaag
     7801 ctctacagcg tcgggcagat cctcaaggaa gacgtgggca gcgacagcac ctggaccagc
     7861 tacaaccagg ccctggggcg gaagttcttt aaggggggaa aatagcccag tagcgacctc
     7921 actttgcaat ttgcaatttg catttctcaa ttcgcaattt gcaattttcc cggtcctcgc
     7981 aagccagcgc ggcatttcac cgaccgaatg caaagtgcaa attggccatc gcaaattgca
     8041 aaattggcat cagccaggcc ctcggccgga ggttcttcaa gagggcgaag ctcacggcgc
     8101 acggcaggga aaccgcggat ttcgctgatg gcgcggatgt cggggaaatg cctcccttca
     8161 tccgtgacat ccgtgcaatc cgcggttgcc gacgcccgga cgactgccag gccctcggcc
     8221 ggaagttctt caaaagggcg aagctcacgg cgcacggcag ggaaaccgcg gatttcgctg
     8281 ctggcgcgga tgtcggggaa atgcctccct tcatccgtga catccgtgca atccgcggtt
     8341 gccacgcccg gacgactgcg tagcatcagg ccatccgccg aatttccggc caattccgct
     8401 cgccgcacca aaaaatctgc atccaaggtg cattttcccg gcagtacctc attagcaacg
     8461 ggggccaacc aatccaacga ctcccaggac cgccgcttcg atggatgcta tcgccccgcc
     8521 gacccctcgc cctgacgtca aaacacaagc cgccatccgc gaggtgctga gcttcctgcc
     8581 gcccgacgtc gtccaggagc tgaacagcgg ccagatcgac ctgcgggacg cggcgtttct
     8641 ggagggcctg gccgaccata tttcccgcct ggcggtggcc agtcccacca cgggccagaa
     8701 ggccctcggc aagttcatca agctcaagaa gttgatcgcc cgctcgctcc gcgactccga
     8761 tcccggcgtc cgcgtctcct cgacgctgca cgccgaccag cgaatcggcc gcaacgccaa
     8821 gtgcccctgc ggcagcggcc ggaagttcaa gcagtgctgc tggcaccgac tctaaacaaa
     8881 cccgaagcgt cagcgaggga cttcgtaagc acgccgtgat taaagaagtc cctcgctgac
     8941 gccttgcggt tagtatccga tttgaacact taccactccc gacccttgca ttccactcct
     9001 gccgatagta agccaatgga taagcctgct ggaggattc
//
LOCUS       DASRJB010000610         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2717967, whole genome shotgun sequence.
ACCESSION   DASRJB010000610 DASRJB010000000
VERSION     DASRJB010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717967"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFB80_16100"
     CDS             complement(<1..233)
                     /locus_tag="VFB80_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN35353.1"
                     /translation="MDLSKVRNFGISAHIDSGKTTLSERILYYSGRIHKMEEVKGDGD
                     GATMDYMDLERERGITITSAATHLEWGGCHMNL"
     gene            complement(676..882)
                     /locus_tag="VFB80_16105"
     CDS             complement(676..882)
                     /locus_tag="VFB80_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35354.1"
                     /translation="MATEPKKQNPKKQAIQMVCAAVGVAVALGAMVALGISGALPGAL
                     FGALGGGGGGLIGWVIASLVVKEP"
     gene            967..>2305
                     /locus_tag="VFB80_16110"
     CDS             967..>2305
                     /locus_tag="VFB80_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN35355.1"
                     /translation="MPNLDSPRGPAPNAPLSIGQYLIRRLQDYGLAHVFGIPGDYILT
                     FYGQLEQSPIEVVGCTREDCAGFAADAYARINGLGAVCVTYCVGGLSVCNSIAGAYAE
                     KSPVVVISGSPGIGERVNNPLLHHKVKDFRTQIEVFEKLCVACTELIDPSSAFREIDR
                     VLDAVVRYKRPGYIEIPRDQVEIVPQIVHTWHHGEPLVEPQVAEEAVEEAAQLLAKAQ
                     RPVIIAGVEIHRFGLQDKVLALAERHQIPIAATILGKSVVPEKHPLYIGLYEGAMGRA
                     EVTQYVEESDCVLLLGAFMTDLNLGIFTAKLDPGRCIYATSEQLRIRHHHYHHVPLDL
                     FLSQLAGRELSVAMRPIPPELRPPRSPFQIQPDAPLTIRRVIARLDEQIDNGTIVIAD
                     IGDALFSASELTIHQRTEFISPAYYTSMGFAVPAAVGALTARPQARVLAIVGDG"
BASE COUNT          411 a          782 c          725 g          387 t
ORIGIN      
        1 atcaggttca tgtggcagcc gccccactcc aggtgcgtgg cggcggaggt gatggtgatg
       61 ccccgctcgc gctccaggtc catgtagtcc atggtcgcgc cgtcgccatc tcccttgacc
      121 tcctccattt tgtggatgcg gccggaatag tagagaatcc gctcgctgag cgtggtcttg
      181 cccgagtcga tgtgggcgga gatgccgaaa ttgcgaactt tcgaaaggtc catactgcta
      241 ccctagaaac gccgggacgg ctcgcaagcg gccgaactgg cacgtactaa atcagccgac
      301 aaggcctaag gccccggccg cacacgacaa cctgcggcgc tggcagccgt cccgcgaggc
      361 gtagcactca atgcacacga cgtgccggga cgattcaagc agcccaattg gtgacgctgg
      421 aaggcgggag agaatccatt cccagcggac agtccagagg cctctctcac tgccgaatcc
      481 ggaactcgcc aacgctccag ccgtcaatga tagccgaaag gttctagctg gaaaggtcaa
      541 atgcccgccc ttggcggttc gcgctgtgaa ttcacgcgag ttttacgatg gaaggagtcg
      601 tagggcccaa tcggcactta cgcttgaaac ttaagtcttt atcaagcaat ggtttgcgcc
      661 gcgccgcctt ggtcttcacg gttccttgac caccagcgag gcaatgaccc aaccgatcag
      721 cccgccgcct ccgccgccga gcgcgccgaa cagcgcgcct ggtagtgctc ccgagatgcc
      781 cagcgccacc atcgccccga gcgcgacggc cacgccgaca gcagcgcaca ccatttgaat
      841 cgcctgcttc ttcggattct gctttttggg ttcggtagcc atggccggaa ttgtaccgca
      901 gcgaccacgc aatctcccga tgccgcctgg gcggctgtcg cgtatctttg ggaaaaggag
      961 ggacgcatgc cgaatctcga ctcgccccgc gggccagctc ccaacgcccc gctgtcgatc
     1021 ggccagtacc tgatccgccg gctgcaggac tatggcctgg cgcacgtgtt cggcattccc
     1081 ggcgactaca tcctgacgtt ctacggccag ctggagcaga gcccgatcga ggtcgtcggc
     1141 tgcacgcgcg aggactgcgc cgggttcgcg gccgacgcct acgcccggat caacgggctg
     1201 ggagccgtct gcgtgacgta ctgcgtgggc ggcctgtcgg tctgcaactc gatcgccggg
     1261 gcctatgccg aaaagtcgcc cgtggtcgtc atcagcggct cgccgggcat tggcgagcgg
     1321 gtgaacaatc cgctgctgca ccacaaggtg aaggactttc gcacgcagat cgaggtgttc
     1381 gagaagctgt gcgtggcctg caccgagctg attgacccct ccagcgcgtt ccgcgagatc
     1441 gaccgcgtac tcgacgccgt ggtccgctac aagcggccgg gctacatcga gattccccgc
     1501 gaccaggtcg agatcgtgcc gcaaatcgtg cacacctggc accacggcga gccgctggtg
     1561 gagccgcagg tggccgaaga ggccgtcgaa gaggcggcgc agctcctagc caaggcccag
     1621 cggccggtga tcatcgccgg cgtcgagatc caccgcttcg gcctgcagga caaggtgctc
     1681 gcgctggccg agcggcacca gattccgatc gccgcgacga tcctgggcaa gagcgtcgtg
     1741 ccggaaaagc acccgctcta catcggcctg tacgaggggg ccatgggccg ggccgaggtg
     1801 acgcagtacg tcgaagagag cgactgcgtg ctcttgttgg gcgcgttcat gaccgacctc
     1861 aacctgggca tcttcaccgc caagctcgat ccgggtcggt gcatctacgc caccagcgag
     1921 cagctccgta tccggcacca ccactaccac cacgtgccgc tggatctgtt cctgagccag
     1981 ctcgccggcc gggagctctc ggtcgcgatg cggccgattc cgcccgagct gcggccgccg
     2041 cgctcgccct tccagattca acccgacgcg ccgctgacga tccggcgggt catagcccgc
     2101 ttggacgagc agatcgacaa cgggacgatc gtgatcgccg acatcggcga cgccctgttc
     2161 agcgccagcg agctgacgat ccaccagcgg accgagttca tcagcccggc ctattacacg
     2221 tcgatgggct tcgccgtgcc cgccgcggtc ggcgcgctga ccgcccggcc gcaggcccgc
     2281 gtcctggcga tcgtcggcga cgggg
//
LOCUS       DASRJB010000611         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1866749, whole genome shotgun sequence.
ACCESSION   DASRJB010000611 DASRJB010000000
VERSION     DASRJB010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866749"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFB80_16115"
     CDS             <1..356
                     /locus_tag="VFB80_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35356.1"
                     /translation="LNPVQGKVLHQNQPLAGALVAFHPDGPRDINVENSVGLTKEDGT
                     FHLTTGQKDGARAGKYTVTIICSEIPQSAKKNLSTAGVDSVDRLKGAYANVDASKIKV
                     EIKTGPNQLEPFDLK"
     gene            587..1177
                     /locus_tag="VFB80_16120"
     CDS             587..1177
                     /locus_tag="VFB80_16120"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35357.1"
                     /translation="MDSFSDLVGRLHAGDDRAAQEVFDRFASRLIGLARSQIAAGIRP
                     KLDPEDVVQSALRSFFRRHEEDRFELQSWDSLWGLLSLITVRKCAKKTEYYLAQRRGG
                     GREISLVAEGDSQAGAVAIDREPTPEDAAMLLETVEEALRDFDPADRQVIELSLQGYS
                     AAEISVQLSRAERSVRRLRERVKKRLWRMIGHEQHD"
     gene            1161..>2911
                     /locus_tag="VFB80_16125"
     CDS             1161..>2911
                     /locus_tag="VFB80_16125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35358.1"
                     /translation="MNSTIDSARPRVRRAVELDSGSWRDCEAAIRRFEAAWQSGPPGI
                     SAYLPLEEPLRSAVLLELVQIDLEFRRKRGEAARFETYLPQFPELARFPNLPEFEQPT
                     AGPDAETRRGSGVAAPPGESLPQLPGFEMLRELGRGCMGVVFLARQTALKRLVAVKMI
                     RGGSRPAEELARFRGEIEAAARLKHQHIVQIYEVGEHAGQPYCVLEYVDGGNLQQKLA
                     GQPQPADVAARIIQQLALAVQHAHEAGIVHRDLKPANVLIEAGGRESGVGSRQGKDPT
                     PDPRLPTPALKIADFGLARHLEAESQQTRTGEIVGTPAYMSPEQAEGASRECGPATDI
                     YALGVILYELLTGRPPFQGASLLDTLEQVRSQEPVPPRALAPRTPRDLNTICLKCLQK
                     EPARRYPTAAALAADLAALADGRPISARAIGPLERAWKAARRRPLVATLAAAVVVIAA
                     AGLSYGIYQYGQTRAALAATATALENEKAERTAKEDALKDARESLADFKAFSTFVVED
                     IVNAARPEGKDGGLGIDATVREALDAATANLAKRFAGQPRAEALARLDLGETYRALGE
                     PAKALAHFQEAVRVRRQV"
BASE COUNT          515 a          975 c          986 g          435 t
ORIGIN      
        1 gcctgaatcc ggtgcaggga aaggtcctgc accagaacca gccgctggcc ggggcgctcg
       61 ttgctttcca tccagatggc ccgcgcgaca tcaacgtcga aaactcagtc ggcctgacca
      121 aagaggacgg cacgtttcac ctgacgaccg ggcaaaagga cggcgcacgt gcgggaaagt
      181 acacggtgac aatcatctgc tccgagattc cgcaatcggc aaagaagaat ttatccacgg
      241 ctggcgtgga tagcgtcgat cgcctcaagg gcgcctacgc caatgtcgat gcgagcaaga
      301 tcaaggtcga aatcaagacg ggacccaatc agctcgagcc gtttgatctg aagtgagcgc
      361 gacccgcgac cgctgcacat ctcagccgat caggatgcct cccggcagaa atttcttgcg
      421 gcagccgtaa caattccgcg ctgcctgcgc tgagcgttgt gctctgtgtc cattcgcgct
      481 atccgccaaa tccgcggtcg acctgtccgg cctgcggcag gcgtttgctt gctccgcggc
      541 gggccgctac gcataatccg atcttccttt ctccagggga aagtccttgg attcattcag
      601 cgatcttgtc ggccggctgc acgcgggaga cgaccgggcg gcgcaggagg tcttcgaccg
      661 ttttgcctcg cggctgatcg gcctggcccg cagccagatc gcggcgggca tccgccccaa
      721 actcgacccg gaggacgtgg tgcagtcggc cctgcggagc ttcttccgcc gccacgagga
      781 ggaccgcttc gaactgcaaa gctgggacag cctgtgggga ctgctttcgc tgatcaccgt
      841 tcgcaaatgc gcgaaaaaga cggaatacta tctggcccag cggcgcggcg gcggtcggga
      901 gatctcgctc gtcgccgagg gcgattcgca ggccggcgcg gtcgccatcg atcgtgagcc
      961 gacgcccgag gacgccgcga tgctgctgga gacggtcgaa gaggccctcc gcgacttcga
     1021 ccccgccgac cggcaggtga tcgagttgag cctgcagggc tatagcgcag cggagatcag
     1081 cgtgcagctg tcgcgggccg agcgctcggt gcggcggctg cgggaacggg tcaaaaaacg
     1141 cctgtggcgg atgatcggcc atgaacagca cgattgacag cgcccggcca cgcgtacggc
     1201 gggcagtcga gctcgacagc ggcagctggc gggactgcga agcggcgatc cgccgcttcg
     1261 aagccgcctg gcaatcgggg ccgccgggaa tatcggccta tttgccgctc gaagagccgc
     1321 tgcggtcagc cgtcctgctg gaactggtcc agatcgacct ggaattccgc cgcaagcgcg
     1381 gcgaggcggc gcgttttgag acctacctgc cgcagtttcc cgagctggcg cggtttccga
     1441 acctgcccga attcgagcag cccacagccg gccccgacgc cgagacccgc cgcggcagcg
     1501 gcgttgccgc gccgccgggc gaaagcctgc cgcagctgcc ggggtttgag atgctgcgcg
     1561 aattgggacg cggctgcatg ggcgtcgtgt tcctcgcgcg tcagacggcc ctcaagcgcc
     1621 tggtcgcggt aaagatgatt cgcggcggct cgcggccggc ggaagagctc gcccgcttcc
     1681 gaggcgagat cgaagctgcc gcccggctca aacaccaaca catcgtccag atttacgaag
     1741 tcggcgagca cgccggccag ccctattgcg tgctggaata cgtcgatggc ggcaacctgc
     1801 agcagaagct ggccggccag ccgcagcccg ccgacgtcgc agcgcggatc atccagcaac
     1861 tggccctcgc cgtgcagcac gcacacgagg cgggcatcgt ccatcgggac ctcaagccgg
     1921 cgaatgtgct gatagaggca gggggtcggg aatcgggggt cgggagtcgg cagggcaaag
     1981 atccgactcc cgatccccga ctcccgactc ccgccctcaa gatcgctgac ttcggcctgg
     2041 cccggcatct ggaagcggaa tcgcagcaga cgcggacggg cgagatcgtc ggcacgccgg
     2101 cctacatgtc gcccgagcag gccgagggcg ctagccgcga gtgcggcccg gcgaccgata
     2161 tctacgccct gggcgtgatt ctgtacgagc tgctcacggg ccggcctccg tttcaagggg
     2221 cgagcctgct ggacacgctg gagcaggtcc gctcgcagga gccggtgccg ccgcgggcgc
     2281 tggccccgcg cacgccccgc gacctgaaca caatctgcct caagtgtttg cagaaggagc
     2341 ccgcgcggcg ctatcccacg gcggctgcgc tcgccgccga tctggccgcg ctggccgatg
     2401 gccgcccgat cagcgcccgc gcgatcggcc cgctggagcg agcctggaag gccgcccgcc
     2461 gcaggccgct ggtggcaaca ctcgcggcgg cagtggtagt catcgcagcc gctggcctgt
     2521 cgtacggcat ctatcaatac ggccagacgc gcgctgcttt ggccgccacc gccaccgcgc
     2581 tcgagaacga aaaagcggag aggaccgcca aagaggatgc tctgaaggac gcccgcgagt
     2641 cgctggcaga tttcaaggcg ttcagcacat tcgtcgtcga agacatcgtg aatgcggccc
     2701 gtccggaagg gaaagacggc ggcctgggca tcgacgcgac ggtccgcgag gccctcgacg
     2761 cggcaaccgc gaacctcgcc aagcgctttg ccgggcagcc gcgggccgag gcgctggccc
     2821 gcctcgactt gggcgagacc taccgcgcgc tcggcgagcc ggccaaggcc cttgcgcatt
     2881 ttcaggaggc agtgcgcgtc cgccggcagg t
//
LOCUS       DASRJB010000612         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_462925, whole genome shotgun sequence.
ACCESSION   DASRJB010000612 DASRJB010000000
VERSION     DASRJB010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462925"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFB80_16130"
     CDS             complement(<1..185)
                     /locus_tag="VFB80_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35359.1"
                     /translation="MNFNRIAAIGWLLCAANSAAAQHAAPVLAEPEAVAASPLQFPSP
                     DTPGAGMVFAPYEGKSP"
     gene            complement(477..953)
                     /locus_tag="VFB80_16135"
     CDS             complement(477..953)
                     /locus_tag="VFB80_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35360.1"
                     /translation="MPRGRIRRQPSGRNLELYHELVCEGRSRHEVAARFRLSRPRVTQ
                     LRGQVAEWVAAMLPPGFAATGDPGRRFHQAISVRRLQLQKACGEFLDEFGGGRGAQGY
                     QHLLAARKRGVLPRTSRLPSRELVQSAVRMARELDDLARVASRGPSAGPQVPAEAG"
     gene            1238..2158
                     /locus_tag="VFB80_16140"
     CDS             1238..2158
                     /locus_tag="VFB80_16140"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35361.1"
                     /translation="MTKPLCGRTAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRM
                     QCQNNLRQVGLAIHNYDSTFGRLPTGGQGTSPTNSTTFDRHSLFTVVLPYLEQGNTYQ
                     QFDLRLAYNESAQNIAAAKQVIATYVCPTKAWRMSPSDQQGFGCTDYAATYYTDLDPA
                     TALPNPALRVDGALVTGGSRLSEVTDGTSNTIFVAEDNGRDERMWANHVYIDPVDGQK
                     RRLWRWAEPDNALGVSKLINNNKAPRGGPASCPWNTNNCGPNDEIFSFHSGGANVLLG
                     DGSVRSLQESIAAAPLRAFITRAGGESVGE"
     gene            2385..>3250
                     /locus_tag="VFB80_16145"
     CDS             2385..>3250
                     /locus_tag="VFB80_16145"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZN35362.1"
                     /translation="MAANSVLKIAVIPGDGTGPEVTAEALKVLAAVSKLEKFAFETQS
                     FDWGGERFLKTGETLPAGGVEQLRKFDCIYLGAVGHPDVKPGILEKGLLLELRFQLDQ
                     YINLRPVKLFKGVECPLVGKGPEHIDFVVVRENTEDMYAGIGGWLKKHTADEVATQTA
                     IYTRKGCERCIRWAFEFTRKRNNPKGKMLTLVAKTNVLTYGHDLVWRTFQDVAKEYPD
                     VKADYNHVDACCMWMVKNPEYYDVIVTTNMFGDIITDLGAMIQGGLGVAAGGNINPDK
                     GGVSMFEPMGGSA"
BASE COUNT          627 a         1056 c         1005 g          562 t
ORIGIN      
        1 caggggctct tgccctcgta gggcgcgaag accatgcccg cgccgggtgt gtccggcgag
       61 gggaactgaa gtggcgacgc ggcaaccgct tcaggctctg ccagcaccgg cgcagcgtgc
      121 tgtgccgcgg cgctattggc cgcgcacagc aaccatccga tcgccgcgat ccgattgaag
      181 ttcaccaacg gtctcctacc tacccgaagc gtgagcgagg gactccggca aagtcagcgc
      241 agcccgcaaa tcctctatcg gcggaaaagt ccgcccagcc ctaacaaatt gtctcgccag
      301 actcgccgcg gtcctcggca ggggtaaggt taattcccat cacctggccg tttttcacgc
      361 cgctccgcgg aaaggctgat ttcacaactc cttgccagaa ttgaacttgc agctgttttc
      421 tgccattgcc aatcctcagc gctataaggg tttgagggaa cgatggggaa ttatccttac
      481 ccggcctctg ctggcacttg cgggcccgcg gaagggccac ggctggcgac ccgtgccagg
      541 tcgtccaatt cgcgagccat ccgcacagcg gactggacca gttcgcggct gggcagccgc
      601 gacgttcgcg gcaggacgcc ccgcttccgg gcagcgagca agtgctggta gccctgtgcg
      661 ccgcggccgc cgccaaactc gtccagaaac tctccacagg ctttttgcag ttgcagtcgg
      721 cgaacggaga tggcctggtg gaaacggcgc ccaggatcac ccgtggcggc gaagccgggc
      781 ggcagcatgg ctgcgaccca ctcggccacc tggccgcgga gctgcgtcac gcgcggccgc
      841 gagaggcgga agcgggccgc gacctcgtgc cgcgaccggc cctcgcagac cagctcgtgg
      901 tacagctcca ggttgcgccc cgacggctgt cgccttattc ttccgcgcgg catgagcgat
      961 cctcccgcgg ccccgccggc gcatcaggcg caagtccgct acggcggagc catgccttca
     1021 ttgtaatcgt ttgcacacct ctgtcaatac acttgttccc taaatgccct gaaatttttc
     1081 ccacccggcc gggcgggccc tcggtagcga tgccgccaat gtaatcgccc ttccagcctg
     1141 gaagagcccc gggttgcgtt cagcgcctcc cgcatgtaca ttcaaacgct cttgcaactg
     1201 tctgacgaag ctaatgagtt ccgcatcgag tccctgtatg accaaaccac tctgcggccg
     1261 cacggccttc acgctggttg aattgctggt cgtgattgcg attatcggag ttcttgtcgc
     1321 cctgcttctg cccgccgtgc aggccgctcg cgaggccgcc cggcgtatgc aatgccaaaa
     1381 caacctgcgg caagtcggcc tggcgattca caactacgat agcaccttcg gccgcctgcc
     1441 caccggcggg caaggcacct ctcctacgaa ctcgacgaca ttcgataggc actcgttgtt
     1501 cacggtcgtg ttgccctacc tggagcaggg caacacctat cagcagttcg acctgcgctt
     1561 ggcctacaac gaatccgcac agaacattgc cgccgcgaaa caggtgatcg cgacgtacgt
     1621 ctgtccaaca aaggcctggc ggatgtcgcc cagcgaccag cagggcttcg gctgcactga
     1681 ttatgccgct acctactaca cggatttgga cccagcgacg gccctgccca atccagcgct
     1741 gcgggtcgat ggcgctctgg taaccggcgg cagccggttg tcggaagtga ccgacggcac
     1801 gagcaatacg atctttgttg ccgaggacaa cggccgcgac gagcggatgt gggccaatca
     1861 cgtctatatc gacccggtcg atggccagaa gcgccggctg tggcgctggg ccgagccgga
     1921 caacgcgctg ggcgtttcga aactaatcaa caacaacaaa gcaccacgcg gcggcccagc
     1981 gagttgcccc tggaatacca acaattgcgg ccccaacgac gaaatcttca gctttcactc
     2041 cggcggggcc aacgtcctcc tgggcgacgg ttccgtccgc tccctgcagg agagcatcgc
     2101 cgccgcgccg ctgcgggcat tcatcacgcg agccggcggc gagtcggtgg gcgaatagct
     2161 tctgtcgctc cggtttccct atcggcccga cgatctcgag tatctatcca agcactgctg
     2221 ctgccgcgac gacgccagcc aagagcaacg cgaccggtgc gcccgcatac gctttcgtgc
     2281 gggagccgca ctccacaaag cccaagttgg cggcagggcg ggcatcgcat agaattcggg
     2341 actctcgaat ttcgaacttt ggtgcgcgag gcgaggacac gatcttggct gcgaattccg
     2401 ttttgaagat cgccgtgata cccggcgacg gcaccggccc cgaagtcacg gcggaggccc
     2461 tgaaagtcct ggcggcggtc agcaagctcg agaaattcgc gttcgaaacg cagtcgttcg
     2521 actggggcgg cgagagattc ttgaagacgg gcgagacgct cccggcgggc ggtgtggagc
     2581 agctccgcaa gttcgactgc atctacctgg gcgcggtcgg ccacccggac gtgaagccgg
     2641 gcattttgga aaaagggctg ctgctggagc tgcggtttca gctcgaccag tacatcaacc
     2701 tgcggccggt gaagctgttc aagggggtcg agtgcccgct ggtcggcaag gggcccgagc
     2761 acatcgactt tgtcgtcgtc cgcgaaaaca ccgaggacat gtacgccggc atcggcggct
     2821 ggctgaaaaa gcacacggcc gatgaagtcg cgacgcagac ggcaatctac acccgcaagg
     2881 gctgcgagcg gtgcatccgc tgggccttcg agttcacgcg gaagcggaac aaccccaagg
     2941 gcaaaatgct gacgctcgtg gccaagacca acgtgctgac ctacggccac gacctcgtgt
     3001 ggcggacatt ccaggacgtg gccaaggaat atccggacgt gaaggccgac tacaaccacg
     3061 tcgatgcctg ctgtatgtgg atggtgaaga accccgagta ttacgacgtg atcgtgacga
     3121 cgaacatgtt cggcgacatc atcaccgacc tgggggcgat gattcagggc ggcctgggcg
     3181 tcgctgccgg cggcaacatc aaccccgaca agggcggagt cagcatgttc gagccgatgg
     3241 gcggcagcgc
//
LOCUS       DASRJB010000613         8277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1027283, whole genome shotgun sequence.
ACCESSION   DASRJB010000613 DASRJB010000000
VERSION     DASRJB010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027283"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="tsf"
                     /locus_tag="VFB80_16150"
     CDS             complement(<1..827)
                     /gene="tsf"
                     /locus_tag="VFB80_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZN35363.1"
                     /translation="MAEINAALVRQLREETSLPMMDCKQALTEAMGDKEKAKDILRKK
                     GLKIQEGRGGRETSFGRFGIYAGVDKKTGGIVELKCESAPVAGSADFIQLANDLAKQL
                     ATGPGAKTADELLDQPSPSQPGKTLRQVKDDLFNRIREVFNVGRMARLEGATGGYSHN
                     SGTVSGVLVSVEGGTDEAAKDVSMHVAAMRPLATTKEEIDPAVVAKEREILREAALAE
                     GKPANIVDKMVEGRLKNFYAEKVLLEQPFVKENSLSVGKYAESKGMQVKKFVHWELG"
     gene            complement(932..1696)
                     /gene="rpsB"
                     /locus_tag="VFB80_16155"
     CDS             complement(932..1696)
                     /gene="rpsB"
                     /locus_tag="VFB80_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZN35364.1"
                     /translation="MSETLVNELIEAGVHFGHRASRWNPKMAPYIYARKNGIHIIDIR
                     ETIRGLLRAKKYLAQVAAQGSLILYVGTKKQAGPAVERESLRCHMPFVAERWLGGCLT
                     NFRTIRSRLSRLEEVEKIRGGEQIATYSKKAQSALAREYRKMYRNLNGIRTLNRMPEC
                     LVLVDPNKEKNALKEARTLGITTVALIDTDCDPDLVDLPIPGNDDGIRSIELIMRHLA
                     EAALVGSQSAAAMKKDKGEKPEPAGVGAAEAKDGNV"
     gene            1946..2959
                     /locus_tag="VFB80_16160"
     CDS             1946..2959
                     /locus_tag="VFB80_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN35365.1"
                     /translation="MKRLSWLVLAGVALVLGFLGLGAINNPQQQQKLKDLIAQLSGGQ
                     SAATTAPPARGNDTIRVASFNIQVFGEAKLSDQASMDTIVAILQNFDLIAIQEVRSIS
                     QDVLPRLVALLNAGGKYQYDYAIGPRLGRTTSKEQYAFVFDTATIEIDRSQLYTVVEE
                     QGDRLHREPLVGLFRARGPAPQQAFTFSLVAVHTDPDEVDSELAALQEVFYAVRDDKV
                     RREDDVIMLGDFNARADGLRELGEVPGLVRVISGSTPTNTARTAQYDNILFHDAATSE
                     FTGRGGVYDFMRDFNLTQDQAERVSDHLPVWAEFSVYEGGRPGPIAARPAVGSRGSGV
                     GGR"
     gene            complement(2972..4255)
                     /gene="mtaB"
                     /locus_tag="VFB80_16165"
     CDS             complement(2972..4255)
                     /gene="mtaB"
                     /locus_tag="VFB80_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZN35366.1"
                     /translation="MSLALKTVTLGCKVNQYETELVREGLARIGYRDAAGGEEADLCV
                     VNTCTVTAEGDAKSRQAIRRLARENPRSRIVVMGCYATRAPAELARLPNVAEVLTDKR
                     EIPDLLGRFGVLDVPTGISQFGGRHRAFVKVQDGCLLRCSYCIIPHVRPKFSSRPLPH
                     VLAEIRRLVANGYREIVLTGIHLGHYGVEGNRNKPREQWLRLATLVRAAAALPGEFRL
                     RLSSIEATEVTRELIAALAENPRRVAPHLHICLQSGSDAVLRRMRRRWSSRMFVDRCR
                     LVRESLDNPAITTDIIVGFPGETEPDFAATCRVARECGFSKIHIFPFSPRRGTPAAEM
                     PDQVSAQIKEDRVQRLEEVERELRAAYYRSLLGRRLTVLVEGPSELNPGGWAGTSCRY
                     APVELRSAKGIASDLVEVKGSRITDCGLRIEATVE"
     gene            complement(4302..5489)
                     /locus_tag="VFB80_16170"
     CDS             complement(4302..5489)
                     /locus_tag="VFB80_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HZN35367.1"
                     /translation="MSAQAKNSRPRRPQVALIVETSLASGRDILSGVARYVREHGPWA
                     IYHEPRSLEEMVPRWLKNWKGDGLIARIQNRQIAKAVAAIGLPTVDVLGVVEGCGFPL
                     VHVDDAAIGTSGADHLLERGFQSFGFLSIEGENWSRFRGRAFAARVAQRGFACQTHEI
                     ARRSAKSGSWERYEAELARWIERLAKPAGVMIASDQLGTALLEACRRAGASVPDDVGV
                     IGVDNDEPLCLVSDPPLSSIWPNHAQVGYEAAKLLDTMLQGQPPPAAPVYLSPGKIVA
                     RLSTNTLAVADRLVAAAARFIREHASDGINVEDVVRDAAVCRSVLQRRFKDVFRRTIH
                     DEIVTARLGRAMELLRDTDLPLALIATKSGFRHQEYMGAVFKQRLNTTPARYRIDSRQ
                     DAV"
     gene            complement(5665..7083)
                     /locus_tag="VFB80_16175"
     CDS             complement(5665..7083)
                     /locus_tag="VFB80_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35368.1"
                     /translation="MNSSSLLTDMCPARRAFLRDAFCGFGSLALASLVSQEQARANPL
                     VAKPAPRPAKAKSVIFLFMAGGPSHIDTFDPKPLLNQLDGQKKPEEFGEAKYQFVRPN
                     ARLLGSKRTFKKYGQCGLEVSDLFPHTAQCIDDLAVIRSCYGDKVVHSAAQYELFSGR
                     TVPGFPSMGSWAVYGLGSESDSLPGYVVMPDPDGALEAGQPMYANGFLPAAYQPTLLR
                     PGASPVRNLDLPPGISLDKRRKTVELIRRLNEASLPAGDSDLAARIAAYDLAFKMQTE
                     APQVFDLAGETQATLDLYGIGQEKTDDYGRRCLLARRLVERGVRFTVVVSGGGPGNKQ
                     WDAHGDIEENHLRMAGETDRAVAGLLTDLKQRGLLNSTLVLWGGEFGRSPEAQGGKGR
                     DHHNIGFTMWLAGGGIRGGQAVGATDAIGLRSIEKPYHLRDIHTTILHQLGFDQHDLT
                     YPHLGRDERLTLVEGKVIREIV"
     gene            complement(7398..>8277)
                     /locus_tag="VFB80_16180"
     CDS             complement(7398..>8277)
                     /locus_tag="VFB80_16180"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35369.1"
                     /translation="WLVDPANPLTARVFANRLWHYQFGQGLVATPNDFGVNGAAPSHP
                     QLLDWLANELVDGSWSHKRVQRLVVSSATYRQSAAIGDLGLGIGDLSARNPKSPISNP
                     KLTDPANRLLWHYPRRRLSAEQLRDALLAVSGRMNTKFGGESVMPPVPKELIELLYDP
                     AQWQVTPDAREHDRRSVYLVAKRNLKLPFMEVFDQPDLQTSCARRISSTHPPQSLELL
                     NGELSNRLAADFAARLRSEAGDDPTKQIERAYRLAAGRPPTERERQIALEFLREQPLS
                     EFALAMFNLNAFLYVD"
BASE COUNT         1399 a         2627 c         2726 g         1525 t
ORIGIN      
        1 ttgcccagct cccagtgcac gaacttcttg acctgcatgc ccttgctctc ggcgtacttg
       61 ccgacggaga ggctgttctc cttcacgaac ggctgctcga gcaggacctt ctcggcgtag
      121 aagttcttca gccggccttc gaccatctta tcgacgatgt tggcgggctt gccttcggcc
      181 agggcggctt cacgcagaat ctcgcgctct ttggcgacga ccgccgggtc gatctcttcc
      241 ttggtcgtgg ccagcggccg catcgcggcg acgtgcatgc tcacatcctt ggcggcttca
      301 tcggtgcccc cttcgaccga caccagcaca ccgctgacgg tgccgctatt gtggctgtag
      361 ccgccggtcg cgccttcgag ccgggccatg cggccgacgt tgaacacttc gcggatgcgg
      421 ttgaacaggt cgtccttcac ctgccgcagc gtcttgcccg gctggctggg cgagggctgg
      481 tcgagcagct cgtcggcagt ctttgcgccg gggccggtgg cgagttgctt tgccaggtcg
      541 ttggccagct ggatgaaatc ggcgctgccg gccaccggag cgctttcgca cttcagctcg
      601 acgatgccgc cggttttctt gtccacgccg gcgtaaattc caaatcggcc aaatgacgtc
      661 tcgcggccgc cgcggccctc ctgaatcttc aggcccttct tgcgcaaaat gtccttcgcc
      721 ttctctttat cacccatggc ttccgtcagc gcctgcttgc agtccatcat tggcaggctg
      781 gtctcttctc gcaactgacg caccagcgcg gcgttgattt cggccatgga tgcctcccgt
      841 agagttcaaa gttcaatgtt caagcttcaa agttcggcca ggttcaacat tgaactttga
      901 accttgaact ggagcggtcc cgaaccgggg actaaacgtt gccatccttg gcttcggcag
      961 cgccgacgcc ggccggctcg ggcttctcgc ccttgtcctt cttcatcgcg gcggcgctct
     1021 gcgagccaac gagcgcggcc tcggccaggt gccgcatgat cagttcgatc gagcggatgc
     1081 cgtcgtcgtt gccgggaatc ggcagatcga ccaggtccgg atcgcagtcg gtgtcgatca
     1141 gggccaccgt cgtgatgccc agcgtccgcg cttccttgag ggcattcttc tccttgttcg
     1201 gatcgacgag caccaggcac tcgggcatgc ggttgagcgt gcggatgccg ttcaggttgc
     1261 ggtacatctt gcggtattcg cgggccaggg ccgactgggc cttcttcgag taggtcgcga
     1321 tctgctcgcc gccgcggatc ttttcgacct cctccaggcg gctcagccga ctgcggattg
     1381 tgcggaagtt cgtcaggcag ccgcccagcc agcgctcggc gacgaacggc atgtggcagc
     1441 ggagactctc gcgctcgacc gccggcccgg cttgcttctt ggtgccgacg tacaggatca
     1501 gcgagccctg cgcggcgacc tgagccagat acttcttggc ccgcaaaagg ccgcggatcg
     1561 tctcgcggat gtcgataatg tggatgccgt tcttgcgggc gtaaatgtag ggcgccattt
     1621 tcgggttcca ccggctggcc cggtggccaa aatggacacc cgcttcaatc agttcgttca
     1681 ccaaggtttc ggacacaaaa cactcctctt ccgcggtggc aaatgacctg gtggcagcag
     1741 ccgccagcag tcacaggata aaccttcgcc gaatgcggaa ctctaatggg gacagggact
     1801 taggaccaca gcgaagtgcg atgatccagt agagaatccg tcaggatacc ggccgcctgg
     1861 gacttggtca acggcgtgca agttcaagcg cggcaacgtc tttggtcgac tcaatcaagg
     1921 aatcgcagct cagggagggg caaacatgaa acggctgtcg tggctggtgc tggcgggcgt
     1981 cgcgctcgtg ctcggctttc ttgggctggg cgcgatcaac aatccccagc agcaacagaa
     2041 gctcaaggac ctgatcgctc aattgtccgg cgggcagtcg gcggcgacca ccgcgccgcc
     2101 tgcgcggggc aacgacacga tccgtgttgc ttcgttcaac attcaggtct tcggcgaggc
     2161 caagctgagc gaccaggcct cgatggacac gatcgtggcg atcctccaaa acttcgatct
     2221 gatcgccatt caggaagtcc gttcgatcag ccaggacgtc ctgccgcggc tggtggccct
     2281 gctcaatgcc ggcggcaagt atcaatacga ctatgcgatc ggcccacggc tgggccggac
     2341 cacgagcaag gaacagtacg cgttcgtctt cgacacggcg acgatcgaga tcgatcgcag
     2401 tcagctctat acggtggtcg aggagcaggg cgaccggctg caccgcgagc cgctggtggg
     2461 attgtttcgg gcccgcgggc cggcgccgca acaggcgttc acgttttcgc tggtcgctgt
     2521 ccacaccgat ccggacgaag tcgattcgga gctcgctgct ctgcaagaag tgttctacgc
     2581 cgtgcgcgac gacaaagtcc gccgcgaaga cgacgtgatc atgctcgggg acttcaacgc
     2641 ccgggctgac ggcctgcgcg agctcgggga agtccccggc ctggtacgcg tgatctcagg
     2701 cagcacgcca acgaacaccg ctcgcaccgc gcagtacgac aacatcctct tccacgacgc
     2761 agccacgagc gaattcaccg gtcgcggcgg cgtgtacgac ttcatgcgcg acttcaacct
     2821 gacgcaagac caggccgagc gcgtctcgga ccacttgccg gtctgggccg agttcagcgt
     2881 ctacgaaggc ggccggccgg ggccgatcgc ggcgcggcca gcagtcggga gtcgggggtc
     2941 gggagtcggt ggtcggtaga tcggtggtcg gtcattccac cgtggcctcg atcctcaatc
     3001 cacaatccgt aatccggctt cctttcacct ctaccaggtc gctggcaata cctttggcgg
     3061 atcgcagctc caccggcgca taacggcagc ttgtccctgc ccagccgccg gggttcagct
     3121 cgcttgggcc ctcgaccagc accgttagcc gccggccgag caacgaccgg tagtaggcgg
     3181 cccgcagctc gcgttcgact tcctccagcc gctggacacg atcttctttg atctgagccg
     3241 atacctgatc gggcatttct gccgcgggcg tgcccctgcg cgggctgaac gggaaaatgt
     3301 ggatcttcga gaagccgcac tcgcgggcca cgcggcaggt ggcggcgaaa tcgggttccg
     3361 tctcgccggg aaaaccgacg atgatgtcgg tggtgatggc cgggttgtcg agcgactcgc
     3421 gaaccagccg gcagcgatcg acgaacatgc ggctgctcca gcggcgtcgc atccgccgca
     3481 gcacggcatc gctgccgctc tggaggcaga tgtgcaggtg cggggcgact cgccgtggat
     3541 tttcggccag cgccgcgatc agctcgcggg tgacttccgt ggcctcgatg ctggagagcc
     3601 gcaggcgaaa ttcgcctggc agcgcagcag ccgcacgaac tagcgtcgcc agccgcagcc
     3661 actgctcgcg cggcttattt cgattgcctt cgacgccgta gtggcccagg tgaatgccgg
     3721 tcagcacgat ctcgcgatag ccgttagcga cgagccggcg aatttcggcg aggacgtgcg
     3781 gcagcggccg gctggagaat ttcggccgga cgtgcggaat gatgcagtag ctgcaccgca
     3841 agaggcagcc gtcctgcacc ttgacgaagg cccggtggcg gccgccgaat tgcgagatgc
     3901 ctgtcggcac gtcgagcacg ccgaagcggc cgagcaggtc gggaatctcc cgcttgtcgg
     3961 tcagcacttc ggcgacgttg ggcagccggg cgagttctgc gggcgctcgc gtcgcgtagc
     4021 atcccatcac gacgattcgc gaccgcgggt tctcgcgggc cagccgccgg atggcctgcc
     4081 ggctcttggc gtcgccctcg gccgtaaccg tgcaagtgtt gaccacgcac aagtcggcct
     4141 cttccccgcc ggccgcgtcg cgatagccaa tccgcgcgag cccctcccgc accagctccg
     4201 tttcgtactg gttgaccttg caaccgagcg tgacggtttt cagcgccagg gacatgaacc
     4261 gtccaacata cggctgcgtc cgcgggctgc ccagtggctc gctagaccgc gtcctggcgc
     4321 gagtcgatgc gatagcgggc cggcgtggtg ttcagccgct gcttgaagac tgcgcccatg
     4381 tactcctggt gccggaagcc ggatttcgtg gcgatcaggg ccagcggcaa atcggtgtcg
     4441 cggagcagct ccatggcccg gccgaggcgg gcggtgacga tttcgtcgtg aatggtgcgg
     4501 cgaaagacgt ccttgaagcg ccgctgcagg accgagcggc agacggccgc atcgcggacg
     4561 acgtcttcga cgttgatgcc gtcgctggcg tgctcgcgga tgaaacgggc cgcggcggcg
     4621 accaggcgat cggccaccgc cagcgtgttc gtcgaaagcc gcgcgacgat cttgcccgga
     4681 gaaaggtaga ccggcgcggc cggcggcggc tggccctgca gcatggtgtc cagcagcttc
     4741 gcggcctcat agccgacctg ggcatggttc ggccagatgc tggagagcgg cggatcggag
     4801 accaggcaga gcggctcgtc attatcgacg ccgatgacgc ccacgtcgtc gggcacgctc
     4861 gctccggccc ggcgacaggc ttcgagcagc gccgtgccca gctggtcgga ggcgatcatc
     4921 acgccggccg gcttcgccag ccgctcgatc caccgggcca gctcggcctc gtaacgctcc
     4981 cacgagccgc ttttggccga acggcgggcg atctcgtggg tctggcaggc gaagccgcgc
     5041 tgggcgaccc gcgcggcaaa ggcccggccg cggaagcgcg accagttttc gccctcgatg
     5101 ctgaggaagc cgaagctttg gaaccctcgc tccagcagat gatcggcgcc gctcgtgccg
     5161 atcgcggcgt catcgacgtg caccagcgga aagccgcagc cttcgacgac gcccagcaca
     5221 tcgaccgtcg ggaggccgat tgcggccacg gccttggcga tctggcggtt ctgaatacgg
     5281 gcgatgaggc cgtctccctt ccagttcttc agccaccggg ggaccatctc ctccaggctt
     5341 cgcggctcgt ggtagatcgc ccacggcccg tgctcgcgga cgtagcgggc cacgccgctc
     5401 agaatgtcgc ggcccgacgc cagcgaagtc tcgacgatca gcgccacctg cgggcggcgg
     5461 gggcgggagt ttttcgcttg cgcgctcacg cagctgcttc ctacaagaat tcccgcccga
     5521 tgctgcctgg gagacgacgt gaagtctgcc tcggcgaatt cgcgtaccgc tttcagcggc
     5581 gatatgcggc aattcttacg agaataggcc ggccggcgaa gaattcaagt acgcgccgga
     5641 attctacggc cttaaccgcg gagatcagac gatttcccgg attaccttgc cttccacgag
     5701 cgtcagccgc tcgtcgcggc ccaggtgggg ataggtgagg tcgtgctggt cgaagcccag
     5761 ctggtgcagg atcgtggtgt gaatgtcgcg gaggtgatag ggcttctcga ttgagcggag
     5821 gccgatggcg tcggtggcgc cgacggcctg gccgcccctg atgccgccgc cggcgagcca
     5881 catggtgaag ccgatgttgt ggtggtcgcg gcctttgccg ccctgagctt cgggcgagcg
     5941 gccgaactcg ccgccccaga ggacgagcgt cgaattcaag aggccacgct gcttgagatc
     6001 ggtcaagagg cccgcgacgg cgcggtcggt ctcgccggcc atccgcaggt ggttctcttc
     6061 gatgtcgccg tgggcgtccc actgtttgtt gcccgggccg ccgccggaga cgacgaccgt
     6121 aaaacgcacg ccgcgctcga ccagccggcg ggccaagagg cagcggcggc cgtagtcgtc
     6181 ggtcttttcc tggccgatgc catacaggtc gagcgtggcc tgcgtctcgc cggccaggtc
     6241 gaagacttgc ggcgcttcgg tctgcatctt gaaggccagg tcgtaggcgg cgatgcgtgc
     6301 ggcaaggtcc gaatcgcccg ccggcagcga agcttcgttg agccgacgga tcaattcgac
     6361 tgtcttgcgg cgcttatcga gcgagattcc cggcggcagg tcgaggttcc gcaccgggct
     6421 tgcgccaggc cgcagcagcg tcggctgata ggcagccggc agaaagccgt tggcgtacat
     6481 cggctgtccg gcctcgagag ctccgtcggg atcgggcatc acgacataac caggcagcga
     6541 atcgctctcg gaaccgaggc catacacggc ccacgagccc atgctgggaa agcccggcac
     6601 ggtccggccg ctgaacagct cgtactgggc ggccgagtgc acgactttgt cgccgtagca
     6661 cgagcggatc accgccaggt cgtcgatgca ctgggccgtg tgcggaaaga ggtcggagac
     6721 ttccaggccg cactggccgt actttttgaa ggtgcgtttg ctgccgagga gccgagcgtt
     6781 gggccgcacg aattgatact tggcctcgcc gaattcttcg ggcttcttct gcccatcgag
     6841 ctgattcaag agcggcttgg ggtcgaacgt gtcgatgtgg ctcggcccgc ccgccatgaa
     6901 cagaaaaatg accgacttcg ccttcgccgg ccgcggcgct ggcttcgcca cgagcggatt
     6961 ggcccgcgct tgctcttgcg aaaccaacga ggccagggcc agcgagccga atccgcaaaa
     7021 ggcgtcacga aggaaagcgc ggcgggctgg gcacatgtcc gtcaataggc tggaggaatt
     7081 catctctgat cagaaacgac acgcgagaat gtgacccgac ggctcgtgaa aatactcggc
     7141 cttccaccaa tgtggatcgt tgacttctcg gaacactacc ccggacggaa gcttgcctgg
     7201 tttagtgagg aacggcttgc gaaggttgac gaaaatcgaa gtctcgtgtg gccacccagc
     7261 atgtgtctcg acaatctcgt ttcccgcttt gatctccgaa tccacgatgt cgcgtagttc
     7321 aggacaaagc tgcgcgagtt ccgactgcct aacatcgttg aatcgatcgg actctgattg
     7381 tggatggact gcactcatca atcgacatac aggaaggcat tcaggttgaa catcgccagc
     7441 gcgaattcgg aaagtggctg ctcgcggagg aattccagcg cgatttggcg ttcgcgctca
     7501 gtcggcggcc ggccggcggc gagccgatac gcccgctcga tttgtttggt tggatcgtcg
     7561 ccggcctcgg atctgagtct tgctgcgaaa tcggcggcca ggcgattgga taactcgccg
     7621 ttgagcagct ccagcgactg cggagggtgc gtgctggata tccgccgggc gcagctcgtc
     7681 tgcaagtcgg gctggtcgaa gacctccatg aacggcagct tcaagttccg cttggcgacc
     7741 agatagacgc tccgccggtc gtgctcgcgg gcgtccggcg tcacctgcca ctgcgccggg
     7801 tcgtagagca gctcaatcag ctctttgggc acgggcggca tgacgctttc gccgccgaac
     7861 ttcgtgttca tccggccgct gacggcgagc agcgcatcgc ggagttgctc ggcggagagg
     7921 cgacggcgag ggtagtgcca gaggaggcgg ttggctgggt cggtcaattt gggattggag
     7981 attggggatt tgggattgcg agcactcaaa tccccaatcc ccaatcccaa atccccaata
     8041 gcagcggatt gacggtaggt cgcactagag acgacgaggc gctgaacgcg cttgtggctc
     8101 cagctgccgt ccaccaattc attcgccagc cagtcgagca actgcggatg gctcggcgct
     8161 gcgccgttga cgccgaagtc gttgggcgtg gcgacgaggc cctggccgaa ctgatagtgc
     8221 cagaggcggt tggcgaagac ccgggccgtg agcggattgg ccggatcgac cagccac
//
LOCUS       DASRJB010000614         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1589915, whole genome shotgun sequence.
ACCESSION   DASRJB010000614 DASRJB010000000
VERSION     DASRJB010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589915"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..549
                     /locus_tag="VFB80_16185"
     CDS             121..549
                     /locus_tag="VFB80_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35370.1"
                     /translation="MFQRFCVSLAGAVCLTLAGCGGGSGEKFTPVTGKVTVGGAPLAS
                     GAVTFHPDGTKGNKTQHIPVGSLDAEGKYKLMSATIEGAPPGWYKVTVVAQQPIDPAN
                     PYAPPKHIINPKFSDAATSGFAVEVKEAAAPGAYDFEVTK"
     gene            complement(798..3722)
                     /locus_tag="VFB80_16190"
     CDS             complement(798..3722)
                     /locus_tag="VFB80_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN35371.1"
                     /translation="MGCRSSVVLVCCLIIGSAASHRWLAAADVASPLTPADAQKAFVL
                     ADSSLRIELAAAEPEVVDPVAIRFDEDGRMWVAEMRDYPLGNGGEGSRIRVLEDKDGD
                     GRFESATTFADKLEFVTGLQPWKGGVFVTLSGAVVYMKDTDGDGRCDLEETWFEGFAE
                     QNTQLRANHPRLALDNWIYVANGLRGGTVVSRKLKDLKPINISGMDFRFDPRTGKAEA
                     VSGNGQFGLCFDDWGNRFVCSNRNPVRHVVIEDRYLKANPEVTVPAVMHDVAAWGEQS
                     RIFPISRAWTTSNLHAGQFTAACGVYIYRGDLLPAEFKGNAFTCDPTGNLIHREIMQP
                     AGPTFTSKAAYEGKEFLASPDEWFRPVNMELGPDGALYVVDMYRCVIEHPDFVPDELK
                     RRPDQRLGDDRGRIWRIVPASLPLPLGEGRGEGLPSRRRPNLSKASNEDLIALLSHPN
                     AWQRETAQRLLLERERQALAPRLRELAKDSPEPQARARALWLLAGIDSLEKDLLLSAL
                     LDSQALLKRQAVLIAEQKMDDEAIAAAVRSRRNDADAAVRFQARLSGGSAAFLRRAVA
                     DADDPWLRASLRMGLKPLSVWGALYPWLAKLNGDDALSPGQLLLIEELGELGGANTRI
                     DRRNTLTLAKDLLLTCPQPSAGLAALAGALRGRNKQGGMIAEFTLQDWQAIAERLVVL
                     TKDDKLRPSAIQVLGYVPDSVGYLTPFVARGQDQGLRVAAIAALARQPGALPWQPLVD
                     GFASDTPPVRRAILDALLSRPERVALLLDAIEAGKIKASELESPQVKRLVEHRDAAIK
                     ERAAKLLASAVPADRAQALADYQPVLKMAGDAKRGQAIFEKNCSACHRIAGIGVNVAP
                     DISDSRTKKLDQLLGDILQPNRAIDNNYLGYTVRLTDGTIATGILTTETATSITLKQQ
                     GGKEAVIARSEIDELRSSGQSLMPEGLERTIPHQDMADLLSFIKNWRYLDGRTPLLPA
                     RD"
     gene            3843..4235
                     /locus_tag="VFB80_16195"
     CDS             3843..4235
                     /locus_tag="VFB80_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35372.1"
                     /translation="MLEIILGIYGLYVLVTGKMKLSATKEVVGAPARLIALIMLAPFP
                     IAFAAGLGVGFVAGMNGQDIGDLRMTLMLMEVGIVVACAIAAFSIAHAIGKPPGAAQP
                     QFPTEFGANPPYPQRPSDPNNPYQPPSI"
     gene            complement(4254..4979)
                     /locus_tag="VFB80_16200"
     CDS             complement(4254..4979)
                     /locus_tag="VFB80_16200"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN35373.1"
                     /translation="MTSIPAQPADRIIAQAIPGGWSDREIAVGPHAFRLFTPTDPDEL
                     LNHLESPEATARPHLADPYWAKLWPAAPLLAEAVVRQVSGRRQPADDPPLSPVLELGC
                     GSGLVGIAALASGCEVTFSDYVPFAVELALENAARNGFPNARGLVLDWRQPLDEQFPW
                     IVAADVTYDRTNIDPLLDVCGKMLAKGGQAWFGDAGRGPAADFLRRAKDRGWSVALFD
                     EADGPGLGPAPGRYQRIVLRRGG"
     gene            complement(4976..6262)
                     /locus_tag="VFB80_16205"
     CDS             complement(4976..6262)
                     /locus_tag="VFB80_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN35374.1"
                     /translation="MRTDMLGFFEECFREHGDAAYFRVGNRRSMLLSHPDDIEQVLVA
                     ENRRFIKNYALRFLQPLLGNGLLLNEGESWLRQRRLIQPAFSRQRIESYIPAMAECTI
                     RLLDRWQSGQTISMQPAMLELTMSIAGRTLLGIDVGEQFNTVSDCLDGVMHDFLGRFR
                     AALPLPYWLPTPQNLRLRATVGRLDRVLQELLSERRRTGFAGDDFLSLLMHARDEDDG
                     RGLSDRQIRDEVVTMFVAGHETTANSLSWTWLLLGQHPEIQVRVQEEVQSVLGQRTPT
                     AADLPQLALCDHVIREAQRLYPPAYVIGRRPVCDVTIGGHLLPKNTNVLMSQWVVHRD
                     PRWWDDPLRFNPDRWASGLAGRLPKYAYFPFGGGPRGCIGNTFAQVEAPLVLAMVAQR
                     FTLELVTAGPVKIQPAVTLRPGQSMQFRVHRRQAGP"
     gene            complement(6325..>6918)
                     /locus_tag="VFB80_16210"
     CDS             complement(6325..>6918)
                     /locus_tag="VFB80_16210"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HZN35375.1"
                     /translation="EARALKRYGATNVTVLHTRKREEADTEAFVKPLRQAKGVWFGGG
                     RQWRFVDAYMGTQAEELLQGVLRRGGVIGGSSAGASIQSQYMPRGSPLGNTDVMADGY
                     ERGLGLLPGVAVDQHFTQRRRQPDMTGIMRRYPQLLGIGIDEATAIVVRDSVAEVAGR
                     NHVQFYDYKSGPPAGETDYAQLKAGQKYDLVQRKIVE"
BASE COUNT         1175 a         2266 c         2303 g         1174 t
ORIGIN      
        1 tcgcgtttgc cgacggctcg gtgcggttcg tcaaggattc gactgcccag cagacctatc
       61 gcgacctttc cacgattgcc ggcggcgaaa acgccgtgga acagtaggaa ggacctgagc
      121 atgtttcagc ggttttgcgt cagcctggcg ggcgcggttt gtttgaccct cgcgggctgc
      181 ggcggaggca gcggcgagaa gttcacgccc gtcaccggca aggtgacggt aggcggcgcg
      241 ccgctggcct cgggcgctgt cacctttcac cccgacggca caaaggggaa caagacccag
      301 cacatcccgg tgggcagcct cgacgccgaa ggcaagtaca agctgatgtc ggcgaccatc
      361 gagggcgcgc cccctggctg gtacaaggtc accgtcgtcg cccagcagcc gatcgatccg
      421 gccaacccct acgcacctcc caagcacatc atcaacccga agttcagcga cgccgcgaca
      481 tccggattcg cggtggaagt gaaggaagcc gccgctcccg gcgcttacga ctttgaggtc
      541 acgaagtagg gctcgaccgg tctcgctgag cggccccctg gcctgggtgg atgtgcaacg
      601 gcctgtcttc tcgcaagccc aggcggccga atggattgcg gcgaattctc gaaaactctt
      661 aagtactact tattagcctt tgaggccgcc tgccgtgctc ccccttccgc cgccaaaatg
      721 gcagcgccgc gctaattgga acgatacagc aaatagtgta ccaatggtca aaattcgtgc
      781 ctcgcccccg tccgcggcta atcgcgagcc ggcaacaagg gcgtgcggcc gtcgaggtag
      841 cgccagttct tgatgaagct gagcaggtcg gccatgtctt gatgcgggat ggtgcgctcc
      901 aggccttcgg gcattagcga ttggccgctg gagcgcagct cgtcgatttc gctgcgggcg
      961 atgacggctt ccttgccccc ctgctgtttg agcgtgatcg aggtcgccgt ctcggtcgtg
     1021 agaatgccgg tggcgatcgt gccgtcggtc aaccgcaccg tgtagcccag atagttgttg
     1081 tcgatcgcgc ggttgggctg gaggatgtcg cccagtaatt gatccagctt cttcgtgcgg
     1141 ctgtcgctga tgtcgggcgc aacgttgacg ccaatcccgg cgatccggtg gcaggccgag
     1201 cagttcttct caaagatcgc ctgcccccgc ttcgcgtcgc ccgccatctt cagcaccggc
     1261 tgataatcgg ccagcgcctg cgccctgtcg gccggcacag cgctggccag cagcttcgcc
     1321 gcccgctctt tgatggccgc atcgcgatgc tcgaccagcc gcttgacctg cggcgactcc
     1381 aattcggagg ccttgatttt gccggcttcg atggcgtcga gcaggagcgc cacccgctcc
     1441 ggcctgctca gcaaggcgtc cagaatcgcc cggcggacgg gcggcgtgtc ggaggcgaag
     1501 ccgtccacga gcggctgcca aggcagggcg ccgggctgac gagccaatgc cgcaatcgca
     1561 gccacccgca gcccttgatc ctggccgcga gccacaaacg gcgtcaggta tcccacggaa
     1621 tcggggacgt agccgagcac ctggatggcc gacggccgca atttgtcatc cttcgtcagg
     1681 acaacgagcc gctcggcgat ggcttgccaa tcctgcagcg tgaattcagc aatcatgccg
     1741 ccctgcttgt tccggccgcg caaagcgccg gccagcgccg ccagaccagc tgacggctga
     1801 gggcaagtca gcagcaagtc cttcgcgagc gtcaacgtgt tccgacggtc aattcgcgtg
     1861 tttgcccccc ccagttcgcc cagctcctcg attagcaaca gttgtcccgg cgaaagcgcg
     1921 tcgtccccgt tcagcttcgc aagccacgga tagagggcgc cccacacgga cagcggcttc
     1981 agtcccatcc gcagcgaagc ccgcagccaa ggatcgtcgg cgtcagcaac ggcgcgacgt
     2041 aagaaagcgg cagatccgcc cgaaagtcgt gcctgaaaac gcacggcggc gtcggcatcg
     2101 ttccggcgac ttcggacggc ggcagcgatc gcttcgtcgt ccatcttctg ctcagcgatg
     2161 aggacggcct gtcgcttcaa gagcgcctgc gaatcaagca gcgcggagag caggagatcc
     2221 ttttccagcg agtcgattcc ggcgagcagc cacagggctc gcgcgcgggc ctgcggctcg
     2281 gggctgtcct tggccagttc gcggagtcgc ggcgccaaag cctgacgctc gcgctcgagc
     2341 agcagccgct gggcggtttc gcgctgccag gcattgggat gggagaggag ggcgatcagg
     2401 tcttcgttgg acgccttgga aagattaggg cgccgccgag atggaagacc ctcaccccgg
     2461 ccctctccca gagggagagg gagcgatgcc ggcacgatgc gccagattcg gccgcggtcg
     2521 tcgccgaggc gctggtcggg gcggcgcttg agctcgtcgg ggacgaaatc gggatgctcg
     2581 atgacgcagc ggtacatatc gacgacgtac agcgcgccat cgggaccgag ctccatgttg
     2641 acgggtcgga accactcgtc gggcgaggcg aggaactcct tgccttcgta agcggccttg
     2701 ctggtgaacg tcgggccggc cggctgcatg atctcgcggt ggatcaggtt gcccgtcgga
     2761 tcgcaggtaa aggcgttccc cttgaactcc gcgggcagca agtcgccgcg gtagatatag
     2821 acgccgcacg cggcggtgaa ctggccggcg tgcaggttcg acgtggtcca ggcccggctg
     2881 atcgggaaaa tccgcgactg ttcgccccag gcggccacgt cgtgcatcac ggcgggtacc
     2941 gtgacctcgg gattggcctt gagatagcgg tcctcgatga cgacgtgccg caccgggttg
     3001 cggttgctgc acacgaaacg gttgccccag tcgtcgaagc agaggccgaa ctgcccgttg
     3061 ccgctgactg cctcggcctt gcccgtccgc gggtcgaagc ggaaatccat cccgctgatg
     3121 ttgatcggct tcaggtcctt gagcttgcgg ctgacgaccg tgccgccgcg caggccgttg
     3181 gcaacgtaaa tccaattgtc gagcgccaac cgcggatggt tggcccgcag ctgcgtgttt
     3241 tgctcggcga acccttcgaa ccaggtttcc tccaggtcgc agcggccgtc gccgtcggtg
     3301 tctttcatat agaccactgc gccggagagc gtgacgaaca caccaccctt ccaaggttgc
     3361 aagccggtga cgaactcgag cttgtcggcg aaagtcgtcg ccgattcgaa ccggccgtcg
     3421 ccgtccttgt cttccagcac gcggatccgc gagccctcgc cgccgttgcc gagcggatag
     3481 tcccgcatct cggcgaccca catccgcccg tcctcgtcga accggatcgc caccggatcg
     3541 acgacctccg gctccgccgc ggccaactcg atccgcagcg agctgtccgc caggacaaac
     3601 gccttttgag cgtcggcggg ggtgagcggg ctcgccacgt ctgcggcggc aagccagcga
     3661 tggctagcgg ccgaaccgat gatcagacag caaacgagaa cgaccgagct tcggcaaccc
     3721 atgcaaatcc tcccgcgaat tggctgcatt gtagcctgcc tgcggtggtt tgacgcagcc
     3781 gcgctggatg cgtagattgg gcacatttga accgcgggcg caatcgattg ggagacgccg
     3841 ctgtgctcga gattatcttg gggatctacg gcttgtatgt gctggttacc ggcaagatga
     3901 aattgtcggc gacgaaggag gtcgtcggcg cgccagccag gctaatcgcg ctcatcatgt
     3961 tagcgccgtt tccgattgcc tttgcggcgg gtctgggtgt tggcttcgtc gcgggaatga
     4021 atggccagga tatcggcgat ctgcgaatga cgctgatgct gatggaagtc ggcattgtag
     4081 tggcgtgcgc gattgcggcg ttcagcatcg cccacgcgat cggcaagccg ccgggggctg
     4141 cacagccgca attcccgact gaattcgggg ccaatccgcc gtatccccag cgcccctcgg
     4201 acccgaacaa tccctatcaa ccgccgtcca tatagctcgg agacggcgac gatctagccc
     4261 ccgcgccgga ggacgatccg ctgatagcgg cctggcgcag gaccgaggcc gggcccgtcg
     4321 gcttcgtcga acagcgcgac cgaccagccg cgatctttcg cgcggcggag gaagtcggcg
     4381 gcgggaccgc ggccggcatc tccgaaccag gcctggccgc ccttggcgag catcttgccg
     4441 cagacatcca gcagcgggtc gatattcgtg cggtcatagg tcacgtcggc cgccacgatc
     4501 caggggaact gctcatccag cggctgccgc caatccagca ccaggccgcg ggcattcgga
     4561 aagccgttgc gagcggcgtt ttccagggcg agctcgaccg cgaacggaac gtaatcgctg
     4621 aacgtgacct cgcagccgct ggccagcgcg gcgatgccga cgaggccact gccgcagcca
     4681 agctcgagga cgggactcag tggaggatca tcggcaggct gacgcctgcc gctcacctgg
     4741 cgaacaaccg cctccgccag cagcggcgcc gccggccaca gcttggccca gtacggatcg
     4801 gccaggtgcg gccgcgccgt cgcttcgggc gattcgaggt gattgagcag ctcgtcggga
     4861 tcggtcggcg tgaacaggcg gaaggcatgc ggaccgactg caatctcgcg gtccgaccag
     4921 ccgccgggaa tcgcctgcgc gatgattctg tcggcgggct gcgcagggat cgacgtcatg
     4981 gaccggcctg ccggcggtgc acgcggaact gcatcgactg gccgggccgc agcgtgaccg
     5041 cgggctggat cttcactggc cctgcggtca ctagctccag cgtgaatcgc tgggcgacca
     5101 tcgccagcac gagcggcgct tcgacctgag cgaaggtgtt gccgatgcag ccgcggggtc
     5161 ctccgccgaa ggggaaatag gcatacttgg gcagccggcc ggcgaggccg ctcgcccagc
     5221 ggtcgggatt gaagcgcagc ggatcgtccc accaccgcgg atcgcggtgg acgacccact
     5281 ggctcatcag tacgttggtg tttttgggca gcaagtggcc gccaatcgtg acgtcgcaaa
     5341 ccggccggcg gccgatgacg taggccggcg gatagagccg ctgggcttcg cggatgacgt
     5401 ggtcgcagag cgctagctgc ggcaggtcgg ccgccgtcgg cgttcgctga ccgagcacgc
     5461 tctgcacttc ctcctgcacg cggacctgaa tctccggatg ctggccgagc agcagccagg
     5521 tccagctcag cgagttggcc gtcgtctcgt gtccggcgac gaacatggtc acgacttcgt
     5581 cgcggatttg ccggtcggaa aggccgcggc cgtcgtcctc gtcgcgagcg tgcatgagga
     5641 gcgagaggaa atcatcgccc gcgaatcccg tccgccgccg ctcgctgagc agctcctgaa
     5701 gcacgcggtc gagccggccg acggttgccc gcagccgcag gttttgcgga gtcggcagcc
     5761 agtagggcag aggcagcgcg gcccggaaac ggccgagaaa atcgtgcatc acgccgtcca
     5821 ggcagtccga caccgtgttg aattgctcgc caacatcgat ccccaagagc gtgcggccgg
     5881 cgatcgacat cgtcagctcc agcatcgccg gctgcatgct gatcgtctga ccggactgcc
     5941 agcgatcgag caggcggatc gtgcattcgg ccatcgccgg gatgtagctc tcgattcgct
     6001 gccgcgagaa cgccggctga atcagccgcc gctgccgcag ccacgattcg ccctcgttca
     6061 gcaacagacc gttgcccaag aggggctgca gaaagcggag ggcgtagttc ttgatgaagc
     6121 ggcggttttc ggcgacgagc acctgctcga tgtcgtcggg gtgcgagagg agcatgctgc
     6181 ggcggttgcc gacgcgaaag tacgcggcgt cgccgtgctc gcgaaagcac tcctcgaaga
     6241 agcccagcat gtccgtccgc atgcgcggca aggctccgag cagccaatgg ccgcgtggtc
     6301 cgggcagcgt gcgggtgggg gccattactc gacgatcttc cgctgcacga ggtcgtattt
     6361 ctggcccgcc ttgagctgcg cgtagtcggt ttcgcccgcc ggcggaccac tcttgtagtc
     6421 gtaaaactga acgtgattcc ggccggcgac ttcggcgacc gagtcgcgga cgacaatcgc
     6481 tgtggcttcg tcgatgccga tgcccaagag ctgcgggtag cggcgcatga tgccggtcat
     6541 atccggctgc cggcggcgct gcgtgaagtg ctgatcgacc gccacgccgg ggagcaaacc
     6601 cagtccccgc tcgtaaccgt cggccatcac gtcggtattg cccagcgggc tgccgcgtgg
     6661 catgtattgc gactggatcg aggccccggc gctggagccg ccgatgacgc cgccgcggcg
     6721 aagcacgccc tggagcagct cctcggcctg cgtgcccatg taggcatcga cgaaccgcca
     6781 ctgccggccg ccgccgaacc acaccccctt tgcctgccgc agcggcttga cgaacgcctc
     6841 ggtgtcggcc tcctcgcgct tgcgggtgtg aaggacggtc acgttcgtcg cgccgtaccg
     6901 cttgagcgcg cgggcttc
//
LOCUS       DASRJB010000615         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_463139, whole genome shotgun sequence.
ACCESSION   DASRJB010000615 DASRJB010000000
VERSION     DASRJB010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463139"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2599)
                     /locus_tag="VFB80_16215"
     CDS             complement(<1..2599)
                     /locus_tag="VFB80_16215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35376.1"
                     /translation="MEKSVRQSSAEPQPNPAAGPAAIDPNRPPGTVQMGRTVAGDLGG
                     VGPQAYQPLPVSGAVFGDYELVSEIARGGMGVVYRARQQSLGRSVALKMILSGQLASD
                     HQVKRFQAEAAAAARLDHSGIVPIYEVGQLGGQHFFSMALVEGGSLQERVARGPLPPR
                     EAAELMQRIAEAVHYAHEQGVVHRDLNPQNVLLDRHGNPKVTDFGLAKRDDGADGLTQ
                     SGELVGTPGYMAPEQALGKTHELGPLVDVYALGGVLYCLLAGRPPFQAASLAETLRQI
                     AEQEPVSPRLLNPGTPLDLETICLKSLHKEPQRRYATARALADDLSRYLRGEPILARP
                     IGFLEKAGRFCRRNPAAAGLIGAAAVLLVAAVTLVVVLSRLSAAQAQAEAQDARLAAM
                     GEAARARETQLYAARQLAATQKYYALLGDARQREIRREPGWTWDNLRDLREAARLNTP
                     AIDALDLQNHAAAALCSFDLRPAGTLKELLHVSAIACSPDGRWVALAQEKHWTSCLVF
                     VLSVPEGRLLHKLSYPSSLVWQAFSSQRQDGCESLAVSPDSQQLAVGTRSGMIHLFDL
                     SATEPKPRSWQADKSATDGLAFHPDRQRLISVSADGQLRFWSLESPEQPLGTIAVGRG
                     SYPVCLGGGETIVAGNRILRGPDYSSSSELESSSHRPVLHPSGRLLGQPSGDDFLVSD
                     VATGRDSRKFIDPTLSAAHETSLWGVSFHQGGAVAASSDGVRLKLWDVAGGRLLTSVY
                     LGSKTPGCVAFTPDGRYLVAAAGGKTLLYEVRWPPAVSVLAHEPLPITAIDIATDGRG
                     LVCLSEGLRGKPSGYQGDVSIWDLATGQRRGFTSLASSGTNREPQRPRVLFDRQGSLL
                     AAASQTGAVR"
     gene            2690..4033
                     /locus_tag="VFB80_16220"
     CDS             2690..4033
                     /locus_tag="VFB80_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZN35377.1"
                     /translation="MHEPTQPAPPAESRSWLDRWHQTPLYLRILGACLLGVLVGIALR
                     ELDAAAKASTNPTFHALKPLAWAEGLAVPSRLILRLLGALAGPMVLLAVVQSLMHAQL
                     PKGSAVRLVSLLILNTLVAILIGLFVANTVQPGRWNAVEAPHEKAKELTTNPVQELLE
                     NVPKSLLGPFTDNGKIPSVIMIALALGIAFRQLPKDRFQTASDLVALGFDTLLIVLYW
                     IIEIIPLAVFGVVASIIASKGFTPFLQLGAFVLAVLLALLLQVTYYLARIRLSSWVRP
                     LDVLRGMRDALVMAFSTASSTATMPVTYACLREKVGLRERSASLGALVGANFNNDGTA
                     LYQAMAALFVAQVTGHNLSLLDQFLVVLTCIAASVGAAGIPEAGAVTILIVFRAVNLD
                     VNYIPILLTVDWFLDRCRTTINVMGDVNVSCILDGKEREEPHEPTDQDRELSRLA"
     gene            complement(4049..5107)
                     /locus_tag="VFB80_16225"
     CDS             complement(4049..5107)
                     /locus_tag="VFB80_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN35378.1"
                     /translation="MISRQADPYNAEPALAKLIAEATTPVKHFYVRNHGPVPKVDSAA
                     FRLRVEGLVNKPAEFTLDEIRGRFGSQTSEATLTCAGNRRLEMNRIMPVPGVQWDAGA
                     IGHARWSGAALSDVLRAAELQDSAKHVWFEGLDPIKEKDGSVAPFGGSIPLDKALAAD
                     VKVLLASAMNDEPLTAEHGFPLRAVVPGFIGARSVKWLTKIVVSDRPSPNHYVAEAYK
                     LVQTDAKDELTAAEPIYAWPVNAAICTPGPADKLKAGRTTIAGYALAAGEPGCSVAKV
                     ELSTDGGTTWSGARLLGESRSYSWRLWTTMADLAAGRHELIVRASDSRASAMPQQAAW
                     NLKGYLYNGWHRVNVDVA"
     gene            complement(5620..6981)
                     /locus_tag="VFB80_16230"
     CDS             complement(5620..6981)
                     /locus_tag="VFB80_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN35379.1"
                     /translation="MAATLTQKPLTLADCDHQPAPYDGPSKAEVLALRKQYLTPGLIT
                     YYREPLLVVEGKMQYVWDETGKRYLDAFAGIVTVSVGHCHPYVVEKVREQVGKLQHTT
                     TIYLHPTIGLFGQKLAEHMPEGSELSVSYFTNSGSEANELAVLMSREFTGRSEVISLR
                     NSYHGGTQGTMSLTAHGTWKFPSNPPAAVKNATPGYCYRCPFGLTYPSCDVRCARDVE
                     QLIRYETSGAPACFIAEPIQGVGGTVTPPPEYFQIVYEIVRRYGGLCIADEVQTGFGR
                     TGAHFWGFENWGVTPDIVTMAKGIGNGAPLAACVTRPEIAKTLTSRVHFNTFGGNPIS
                     MTQGLATLEVIDRENIQENARTVGEHLKNRLLDLQERQPLIGEVRGLGLMLGVELVRN
                     RASKEPASSETLDVLERCKERGLLIGKGGLFGNVLRIKPPMCLTKDDADFLVDCLDEV
                     LSR"
BASE COUNT         1144 a         2382 c         2327 g         1250 t
ORIGIN      
        1 gccggaccgc gccggtctga cttgccgccg ccagcaagct gccctggcga tcgaacagga
       61 cgcgcggccg ctgtggctcg cgattggttc cgctagacgc cagacttgta aagccgcggc
      121 gctggccggt tgccaggtcc caaatactca cgtcgccctg gtagcccgag ggcttgccgc
      181 gcaggccctc gctcaggcac accaggccgc gcccgtcggt cgcgatgtcg atcgccgtga
      241 tcggcagcgg ctcgtgggcc agcacgctca ccgctggcgg ccagcgcact tcatacagca
      301 gcgtcttgcc gccggccgcg gccaccaggt agcgcccgtc gggcgtgaag gccacgcagc
      361 cgggcgtctt cgagcccaga tagaccgacg tcagcagccg gccgccggcg acgtcccaaa
      421 gtttcagccg cacgccgtcg gacgaagcag cgactgcgcc gccctgatgg aagctaacgc
      481 cccacagcga cgtctcgtgg gccgcggaca atgtggggtc aatgaacttc cgcgagtcgc
      541 gccccgtggc aacatctgac acgaggaagt cgtcgccgct cggctggccc agcagtcgcc
      601 cgctcggatg cagcactggg cgatgcgagc tgctctccag ttcgctggaa ctcgaataat
      661 cgggtccgcg cagaatccgg ttgccggcga cgattgtctc gccgccgccg agacacacgg
      721 gatacgaccc gcggcccacg gcgattgtcc ccagcggctg ctcgggggac tcgagcgacc
      781 aaaatctgag ctgtccgtcc gcggagacag agatcagccg ctgccgatcg ggatgaaacg
      841 ccaggccgtc ggtcgccgac ttatccgcct gccaggagcg cggctttggt tcggttgccg
      901 agaggtcaaa caggtggatc atgccgctcc gcgtgccgac ggccagttgc tggctatccg
      961 gactgaccgc cagcgattcg cagccgtctt gccgctgcga gctgaaagcc tgccacacga
     1021 gcgacgaggg ataggagagt ttgtgcaaca agcgtccctc gggaacgctg aggacgaaca
     1081 cgaggcagga ggtccagtgt ttttcttggg cgagggcgac ccagcggccg tcgggcgagc
     1141 aggcaatggc cgacacgtgg agcaattcct tgagcgtgcc cgccgggcga aggtcgaagc
     1201 tgcagagggc cgccgcggca tggttttgca ggtccaaggc atcgatcgcg ggcgtgttca
     1261 gccgcgcggc ctcccgcagg tcgcggaggt tgtcccaggt ccagcccggc tcgcgccgaa
     1321 tttcgcgctg ccgcgcgtcg cccagcagcg catagtattt ttgcgtggcc gcgagctgcc
     1381 gcgccgcata gagctgcgtc tcgcgggctc gggcggcttc gcccatcgcg gccaatctgg
     1441 cgtcttgtgc ttcggcctga gcctgtgccg cgctcagtcg cgacaggacg accaccagcg
     1501 tcaccgcggc gaccagcaag accgcggcag cgccgatcag accggccgca gccgggttgc
     1561 ggcggcagaa ccgtccggcc ttttccagga agccgatggg ccgggccagg atcggctcac
     1621 cccgcagata gcgcgacagg tcgtcagcca gagcccgcgc cgtggcgtag cggcgctgcg
     1681 gctccttgtg cagcgacttg aggcagatcg tttccaggtc gagcggcgtg cccggattca
     1741 gcagccgcgg cgagaccggc tcttgctcgg cgatctgccg cagcgtttcg gccaggctcg
     1801 cggcctgaaa cggcggccgc cccgcgagca ggcaatagag cacgccgccg agggcataca
     1861 catcgaccag cgggcccagc tcgtgcgtct tgcccagggc ctgctcgggg gccatgtagc
     1921 caggcgtgcc gacgagctcg ccgctttgcg tcaaaccatc ggccccatcg tcccgcttgg
     1981 ccagtccgaa gtcggtgacc ttcggatttc cgtgccggtc gagcaggacg ttttgcgggt
     2041 tgaggtcgcg gtggaccacg ccctgctcgt gggcgtagtg gactgcctcc gcaatccgct
     2101 gcatcagttc ggcggcctcc cgcggcggca gcggcccgcg ggccacgcgc tcctggaggc
     2161 tgcccccttc gaccagcgcc atcgaaaaaa agtgctgccc gccgagctgg ccgacctcgt
     2221 agatcggcac gatgccgctg tggtccaggc gggccgcggc ggccgcctcc gcctggaagc
     2281 gcttgacctg atgatccgag gccagctgcc cgctcagtat catcttcaag gcgaccgagc
     2341 ggcccagcga ctgctggcgg gcccgataaa ccacgcccat gccgccgcgg gcgatttccg
     2401 ataccagctc gtagtcgcca aagaccgcgc cgctgacggg caacggctga tacgcttgcg
     2461 gaccgacgcc gcccaaatcg ccggccacgg tccgccccat ctgcacggtt cccggcggcc
     2521 ggttgggatc gatggccgcc ggacccgcgg ccgggttcgg ctgcggctcg gcgctcgatt
     2581 ggcgcacgga cttttccatc gctccgtcca agacgaggtt cgctggccag attgctgccg
     2641 gtctgcgtca tccttaggct actgctcacc cgccaaccac ggcaagtcta tgcacgaacc
     2701 gacgcagccc gctccccctg ccgaatcccg gtcctggctc gaccgctggc accagacgcc
     2761 gctttatctg cggattctgg gggcctgcct gctgggcgtc ctcgtcggca tcgcccttcg
     2821 cgagctggac gcggcggcaa aagcctccac taaccccacc ttccacgctc tcaagccgct
     2881 ggcctgggcc gaggggctgg cggtgcccag ccggctgatc ctgcggctct tgggcgcgct
     2941 ggccgggccg atggtgctct tggccgtcgt gcagtcgcta atgcacgccc agctgccgaa
     3001 gggaagcgcc gtgcggctgg tgagcctgct gattctcaac acgctggtgg cgatcctgat
     3061 cggcctgttt gtggccaata cggtgcagcc cggccgctgg aacgcggtcg aggcgcccca
     3121 tgagaaggcg aaagaactga ccacgaatcc agtccaggag ctgctcgaaa acgttccgaa
     3181 gagcctcctg ggcccgttca ccgacaacgg caagattccg tccgtgatca tgatcgccct
     3241 ggcattgggg atcgcctttc ggcagctgcc gaaggaccgt tttcaaaccg cgtccgacct
     3301 ggtcgcgctc ggcttcgata cgctgctgat cgtgctgtat tggatcatcg agatcattcc
     3361 gctggcggta ttcggcgtcg tggccagcat tatcgcgagc aagggcttta cgccgtttct
     3421 tcagctgggt gcgttcgtgc tggccgtcct cctggccctc cttttgcaag tgacttatta
     3481 cctggcacgc atccggctca gctcgtgggt gcgcccactc gatgtgctgc gcgggatgcg
     3541 cgacgcactg gtcatggcgt tctcgacggc cagttcgacg gcgacgatgc cggtgactta
     3601 cgcctgcctg cgcgagaagg tcggcctccg cgagcgctcc gccagcctgg gcgctctggt
     3661 cggcgcaaac ttcaacaacg acggcacggc cctctatcag gcgatggccg cgctgttcgt
     3721 ggcccaggtc accgggcaca acctgtcgct gctcgaccag tttctggtcg tgctcacctg
     3781 catcgccgcc tccgtcggcg cagcgggcat tcccgaggcc ggcgcagtga ccatcctcat
     3841 cgtgtttcgg gccgtgaacc tggacgtcaa ctacatcccc atcctgctga ccgtcgattg
     3901 gttcctcgat cgctgccgca cgacgatcaa cgtcatgggc gacgtgaacg tgagctgcat
     3961 cctcgacggc aaagagcgcg aggagcccca cgagccgacc gaccaggacc gggaattgtc
     4021 tcgcctggcg taacgccgcg gcgaccggtc aggccacgtc cacgttgacg cggtgccagc
     4081 cgttgtagag gtagcccttg aggttccacg cggcttgctg cggcatcgcg ctagcgcggg
     4141 agtcgctggc ccggacgatg agctcgtgac ggccggccgc gaggtccgcc atggtcgtcc
     4201 acagccgcca gctgtaagac cgcgactcgc ccagcagccg agccccagac caggtcgttc
     4261 cgccgtcggt cgagagctcg accttcgcca cggagcagcc gggctcgccc gcggccagtg
     4321 cgtagccggc gatcgttgtg cggccggcct tgagcttgtc ggccgggccc ggcgtgcaga
     4381 tggcggcgtt gaccggccag gcatagatcg gctcggcggc ggtcaactcg tctttggcgt
     4441 cggtctgcac cagcttgtag gcctcggcga cgtagtgatt cggcgaaggg cggtcgctga
     4501 cgacgatttt ggtcagccac ttcacgctgc gagcgccgat gaatccgggc acgaccgcgc
     4561 ggagcggaaa gccgtgctcg gccgtgagcg gctcgtcgtt catggcgctg gccagcagca
     4621 ctttcacgtc ggccgccaag gccttgtcga gcgggatcga gccgccgaac ggcgccacgc
     4681 tgccgtcctt ctccttgatg ggatccaggc cttcgaacca gacgtgcttc gccgagtctt
     4741 gcagttcggc ggcccgcagc acgtcggata aggcagcgcc ggaccagcgg gcgtggccga
     4801 tcgcgccggc atcccactgc acgccgggga ccggcatgat gcggttcatc tccaggcggc
     4861 ggttgccggc gcaggtgaga gtcgcctcgc tggtctgcga gccgaaccgc ccgcgaattt
     4921 catccagcgt gaactccgcc ggcttgttga ccagcccttc gacgcgcagg cgaaacgcag
     4981 ccgaatccac cttgggcaca ggcccgtggt tgcggacata aaagtgcttc acgggcgtcg
     5041 tggcctcggc gatcagcttc gccagcgcgg gctcggcgtt gtaagggtcc gcctggcggg
     5101 agatcaaatc tgacgtctcg gctgccctgg cttgccggct caggccacag gatgctgcga
     5161 gcgccgaagc ggtttgcaaa aaccggcgac gagtaatctg ttggaagtac ggcatcgcat
     5221 gactccccgt tgcgcttcta ggccagcggc tggcaagtcg aacctcgttg gattaatcgc
     5281 caggtccacc gcatccgtgg cgaataattc gggccgcgct cgcctgcgcc cgtgtcataa
     5341 gccgtttcgc ctcttgggtt tgcgaagacc gcttttgcgg cgtgaggacg aataattcgc
     5401 cattggggcc gaaaacctgg ggatctggcg aacaatggct ggaaaaactc ggtaaggtct
     5461 tggcctgcca gattttagtt gtcgtccgca cgtggcgaaa aattcggctg gcgaaggtat
     5521 cgatagcggc aaacacggga tcggtccggg tcaaaatggc agaccggcgc atcgcttgga
     5581 acgtaactgc aaatagcgta ccactgtaca caaatccgtc tatcgcgaca gtacttcgtc
     5641 caggcaatcg acgaggaaat cggcgtcgtc cttggtcaga cacatgggcg gcttgatgcg
     5701 gaggacattg ccgaacaggc cgcccttgcc gatcaacagg ccgcgctcct tgcaccgctc
     5761 cagcacgtcg agcgtctcgc tgctggcggg ttctttcgac gcccgattgc ggaccagctc
     5821 gacgcccagc atcaggccca ggccgcggac ttcgccgatc agcggctgcc gctcctgcaa
     5881 atcgagcagg cggtttttca gatgctcgcc gacggtgcgg gcgttttcct ggatgttctc
     5941 gcggtcgatg acttccagcg tggccaggcc ctgcgtcatc gagatcgggt tgccgccgaa
     6001 ggtgttgaag tggacgcggc tcgtgagcgt cttggcgatc tccgggcgcg tgacgcacgc
     6061 agccaagggc gcgccattgc cgatgccctt ggccatggtc acgatgtccg gcgtcacacc
     6121 ccagttctcg aagccccaaa agtgcgcgcc ggtgcggccg aagccggtct gcacctcgtc
     6181 ggcgatgcac aggccgccgt agcggcggac gatttcatag acgatctgaa agtactccgg
     6241 cggcggcgtc accgtgccgc cgaccccctg gatcggctcg gcgatgaaac aggccggcgc
     6301 gccgctggtc tcatagcgga tgagctgctc gacgtcgcgg gcgcagcgga catcgcagct
     6361 gggataggtc aggccgaacg ggcaccggta gcagtagccg ggcgtggcgt tcttcaccgc
     6421 cgcgggcgga ttgctgggga atttccaggt gccatgggcg gtcagcgaca tggtcccctg
     6481 cgtgccgccg tggtagctgt tccgcagtga gatcacctcg gagcgccccg tgaattcccg
     6541 cgacatcagc accgccagct cgttcgcctc gctgccgctg ttagtgaaat acgagacgga
     6601 cagctccgaa ccctccggca tgtgctccgc gagcttttgc ccgaagaggc cgatcgtcgg
     6661 atgcaggtag attgtcgtcg tgtgctggag cttgccgacc tgctcgcgga ctttctccac
     6721 gacgtaggga tggcagtggc ccaccgagac ggtcacgatt ccggcaaatg catccaggta
     6781 ccgcttgccc gtctcgtccc agacgtactg catcttccct tccaccacga gcagcggctc
     6841 gcggtagtaa gtgatcagcc ccggcgtcag gtactgcttc cgcagcgcca ggacctccgc
     6901 cttgctcggg ccgtcgtagg gcgctggttg atgatcgcag tcggcaaggg ttagcggttt
     6961 ctgagtcaga gttgcggcca ctgttcgtct ccttaccttg gcactcattg tacgaagaga
     7021 taaccgcccc gcgttttcaa ttcaatgcac aggttcgcca acgcttcacc gacattctcg
     7081 ccatcgacat tgacggcaga aat
//
LOCUS       DASRJB010000616         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1027623, whole genome shotgun sequence.
ACCESSION   DASRJB010000616 DASRJB010000000
VERSION     DASRJB010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027623"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1253
                     /gene="nadA"
                     /locus_tag="VFB80_16235"
     CDS             108..1253
                     /gene="nadA"
                     /locus_tag="VFB80_16235"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZN35380.1"
                     /translation="MPALLEPPAAPLRELKPYKSLADEELSARIEAVRQQFGQRLLIL
                     GHHYQQDEVIRHSDLRGDSYQLSQQAASSRECRTIVFCGVHFMAETADILANRPQKLA
                     ERGGERVTVILPDMAAGCSMADMAAIDQVEAAWQDLGEVIDTNDLTPVTYINSAASLK
                     AFCGRHGGIVCTSSNARAVLEWSFKRTKRVFFFPDQHLGRNTALKMGITNAQMPVYDP
                     HGGDLGGSSEEAIAGSRVILWKGHCSVHQMFRAEHVALFRQRYPGIKILVHPECPQEV
                     NDIADVSGSTSKIIQTVEAAPPGTKWAIGTELHLVNRLKHEHPEQEIHFLSPVVCMCA
                     TMYRIDLAHLCWSLENLAAGHAVNVIDVDDEIARWSLASLERMLEVK"
     gene            complement(1306..1821)
                     /locus_tag="VFB80_16240"
     CDS             complement(1306..1821)
                     /locus_tag="VFB80_16240"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HZN35381.1"
                     /translation="MSAPSQFSPPPSSGGGSSALKIILIVVAVLVLGCAGLCAGCLYF
                     AGRTAQEAGKVYEELNKLGGAYTNTQAAVSNDPQVIDRLGQPILMTSPAKRENTGEFK
                     TSGETFQFDIKGSKGTAIVSAVAAAPDKTSAFKVTKITVTFSDGSVVDVQPPSEQFTP
                     SELKIDTGEAK"
     gene            complement(1871..2770)
                     /locus_tag="VFB80_16245"
     CDS             complement(1871..2770)
                     /locus_tag="VFB80_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35382.1"
                     /translation="MIFDHIEKLKRQLTDKYVVADVSHPELQRFRGLTGMVKTVNMSG
                     RALVEFDGYNNIGWYDIDPSFLKVVDAPAPKPVESKKDKAAAPKAESPKAPPAASKPV
                     GEKKTPAPAAKAAAPAAGKPSVAEILAAARRPASAAAAPVAKAKPAAPAKAAPGGKGM
                     SMAEILAAARGKGGPAPAAKAEAAPAAEKPAPKAKPAPKPAPAEKPAATESVKAAPGG
                     KGMSMAEILAAARGKGGAAPAKSAPPPEPVQESPPAAEEPAAAAPAADEPAAAPAAAS
                     GSLKGKITSVAEQLAYCRKADAK"
     gene            complement(2843..3430)
                     /locus_tag="VFB80_16250"
     CDS             complement(2843..3430)
                     /locus_tag="VFB80_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN35383.1"
                     /translation="MRRALVSAGLAIVGSEGIEALSLRAVARRAEVSHSAPYHHFQDK
                     AELLAAVAAAGFEQLVAAIDAQAAESQARTALDALRAVGRGYLRFAVGHPAIFRLMFR
                     PELTRPAEHPVLVAAEALAFGKLLQTVAMCQQTGELPGHDPLPPSAACWSIVHGLAML
                     KVEQVLGETPLGGIPFEQLVAAVVETVIAGVRARP"
     gene            3581..3952
                     /locus_tag="VFB80_16255"
     CDS             3581..3952
                     /locus_tag="VFB80_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN35384.1"
                     /translation="MPKNAIHWFEIPTRDIQRAVRFYNRVLNTEMAAMDMMGCQMAMF
                     PCEQGVGVGGALVQHEQQAPAAQGTLVYLDGGDDLAVPLGKVEAAGGKVAMPKTSIGE
                     HGFCAMFIDTEGNRVGLHSMK"
     gene            4047..4748
                     /locus_tag="VFB80_16260"
     CDS             4047..4748
                     /locus_tag="VFB80_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35385.1"
                     /translation="MSVLLLVAALVGQSGSQFQDNSTLIRSRPTPSAERGFLDYQREL
                     SSLLRQEATAKDNAARARAIRALCDLHGRLVGDSRYSTSDTLKEYRSRLWSRLTKVKT
                     DLKREFGRDADKRQSLEDAAVLESADALSAVAADSLAASLSLSGQTQGGPAYLLGGGA
                     GTGALGGGAGTGDYGPQLVALIERTINPAFWDTVGGPGAIVYYQPLQCLVVRATSEIH
                     GNLGGLLGGLRAAGP"
BASE COUNT          774 a         1600 c         1638 g          814 t
ORIGIN      
        1 gcgcttgaag gatgtgaaac agcggccgga tgtcagcgat tcgccagcga cacggaaatg
       61 aatcgcgtat aattgcagga gtccgttcct cgctgctttg ctgtgccatg cccgccctgc
      121 tcgaaccgcc ggccgccccg cttcgcgagc tgaagcccta caaatcgctc gcggacgagg
      181 agctctctgc gcggatcgag gccgtgcggc agcagttcgg ccagcggctg ctgatcctgg
      241 gccaccatta tcagcaggac gaagtcatcc ggcacagcga cctccgcggc gacagttacc
      301 agctcagcca gcaggccgcc tccagccgcg agtgccgcac gatcgtcttt tgcggcgtgc
      361 acttcatggc cgagacggcc gacatcctcg ccaaccggcc gcagaagctg gccgagcgcg
      421 gcggcgagcg ggtcacggtc atcctgcccg acatggccgc cggctgctcg atggccgaca
      481 tggcggcgat cgaccaggtc gaggcggcct ggcaggacct gggggaggtc atcgatacca
      541 atgacctgac gccggtcacg tacatcaact cggcggcgag cctcaaggcg ttctgcgggc
      601 ggcacggcgg catcgtctgc acgtcgagca acgcccgcgc ggtgctggag tggtccttca
      661 aacgcaccaa gcgggtcttc ttctttcccg accagcacct gggccgcaac acggcactga
      721 agatgggcat cacgaatgcc cagatgccgg tctatgaccc gcacggcggc gatctgggcg
      781 ggagcagcga ggaggccatc gccggcagcc gcgtgatcct ttggaagggg cactgctcgg
      841 tgcaccagat gttccgggcc gagcacgtgg cgctgttccg gcagcgctat ccgggaatca
      901 agattctggt gcatccggaa tgcccgcagg aagtgaacga catcgccgac gtttcgggct
      961 cgaccagcaa gatcatccag acggtcgagg ccgcgccgcc gggcacgaag tgggcgatcg
     1021 gcaccgagct gcacctggtc aatcggctca agcacgaaca cccggagcag gagattcact
     1081 tcctctcgcc ggtcgtctgc atgtgcgcga cgatgtatcg catcgacctg gcccacctct
     1141 gctggagcct ggagaacctg gccgcggggc acgccgtgaa cgtcatcgac gtggatgacg
     1201 agatcgcccg ctggagtctc gccagcctgg agcggatgct cgaagtgaag tagtggggca
     1261 gacatacttg tctgcctcgc gccaggctag aaagcctgcc cgacgctact ttgcctcgcc
     1321 cgtgtcgatc ttcagctcgc tcggagtgaa ctgctcgctc ggcggctgca catccacgac
     1381 ggacccgtcg ctgaacgtga cggtgatctt cgtcaccttg aatgcgctgg tcttgtcggg
     1441 ggcagccgcg accgcactga cgatggccgt gcccttggac cccttgatgt cgaactggaa
     1501 ggtctcgccg gacgttttga actcaccggt gttttcccgc ttcgcggggc tggtcattag
     1561 gattggctgg cccaagcggt caatcacctg agggtcgttg cttacggctg cttgggtgtt
     1621 tgtgtaagcg ccgcccagct tattcagctc ctcgtaaact ttcccagctt cctgggcagt
     1681 ccggcccgcg aaatagagac agccggcgca gaggccggcg cagccgagca ccaggacggc
     1741 cactacgatg aggatgatct tgagagccga actgccgccg ccggaggatg gcgggggact
     1801 gaactggctg ggggcgctca tcggcgtact ccgttagggg tgccggcggg agcgtcgccc
     1861 tcccgccgcg ctatttcgca tccgccttgc gacaataggc gagctgctcg gcgaccgacg
     1921 tgatcttgcc tttaagcgag ccgctcgctg ctgccggtgc agccgcgggc tcgtcagccg
     1981 ccggcgcagc cgcagcaggt tcttcggcgg ccggcggcga ttcttggacc ggctcgggcg
     2041 gaggggcgga tttcgccggt gcggccccac ccttcccgcg ggcagccgcc aggatctccg
     2101 ccatgctcat gcctttgccg cccggtgcgg ctttcaccga ttcagttgcc gcgggctttt
     2161 cggcgggggc gggcttgggg gcgggcttgg ccttgggcgc gggcttctcc gccgcgggcg
     2221 cggcttccgc cttggctgcc ggcgctggac cccctttgcc gcgagcggcg gccagaatct
     2281 cggccatgct catgcccttg ccacccggcg cggccttcgc gggggccgcc ggcttggctt
     2341 tcgcaaccgg cgcagcggca gcgctggccg gtcggcgcgc agcggcgagg atctcggcca
     2401 ccgaaggctt gcccgcggct ggtgcagccg ccttcgccgc cggagcgggg gtcttctttt
     2461 cgccgacggg cttggacgcg gccggcggag ctttgggcga ttcggccttc ggcgctgcgg
     2521 ccttgtcctt cttcgattcg accggcttgg gtgcaggagc atcgacgacc ttcagaaagc
     2581 tcggatcgat gtcgtaccag ccgatgttgt tgtagccgtc gaactcgacg agcgcccggc
     2641 cgctcatgtt gacggtcttg accatgccgg tcagaccacg gaaccgctgc aactccggat
     2701 gcgacacgtc cgccacgacg tacttgtcgg tcagttgccg tttcagcttc tcgatgtggt
     2761 cgaagatcat ggtcgcggac gaggacggcg cttagcgaga attcatgggg aaccgccatt
     2821 gtgggagccg gctggcgatc catcaaggcc gggcgcgaac gccggcgatc accgtctcga
     2881 ccaccgctgc cacgagctgt tcgaagggga tgccgcccag cggcgtctct cccaggacct
     2941 gctcgacctt gagcatcgcc agtccgtgca cgatgctcca gcatgcggcg ctaggcggca
     3001 gcggatcgtg gccgggcagc tcgcccgttt gctggcacat ggccaccgtt tgcagcagct
     3061 tgccgaatgc cagggcttct gccgcgacga gcaccggatg ctcggccggc cgcgtcagct
     3121 cggggcggaa catcagccgg aagatggccg gatgtccgac tgcgaatcgc aggtagccgc
     3181 ggccaacggc ccgcagggcg tcgagcgcgg tgcgggcctg cgactcggcc gcctgggcgt
     3241 cgatggcggc gacgagctgt tcgaagccgg ccgccgcgac cgccgcgagc agctcggcct
     3301 tgtcctgaaa gtggtgatac ggggcgctgt gcgagacctc ggcgcggcga gcgacggccc
     3361 gcaaagacag cgcctcgatc ccctcgctgc cgacaattgc caagcccgcc gatacgagcg
     3421 cacggcgcaa atcgccgtgg tgatagggag gctttgacga acggctctgt ggaggcggct
     3481 ttggcttggt cacagcgtgc ggcaaaactt gacatcgtaa agatgcgcgc gtaggatcgt
     3541 ccgcgatccc aacgctcatt ctcacagcga ggcgccaccg atgcccaaga acgccattca
     3601 ctggttcgag atacccacgc gggatatcca acgagcagtg cgattctaca accgagtact
     3661 gaataccgaa atggcagcta tggacatgat gggctgccag atggccatgt ttccgtgcga
     3721 gcagggcgtg ggcgtcggcg gcgcgctggt ccagcacgag cagcaggctc ccgccgcgca
     3781 gggcacgctg gtctatctcg acggcggcga cgacctggcc gttccgctgg gcaaggttga
     3841 ggccgccggc ggcaaggtgg ccatgcccaa gacgtcgatc ggcgagcacg gcttctgcgc
     3901 gatgttcatc gacaccgaag gcaaccgcgt cggattgcac tcgatgaagt aagcagtgtt
     3961 gcccacgctg cgcttcagcc gatacactaa cagtggcatc ctcggctggg ggcagcgtgc
     4021 gggatgccgg cagagggaga cctctcatgt cggttcttct cttggtggcg gccctggtcg
     4081 gtcaaagcgg cagccagttt caggacaact ccacgctcat tcgctcgcgg ccgacgccga
     4141 gcgccgagcg gggctttctc gactatcagc gcgagctgag cagcctcctg cggcaggagg
     4201 cgacggccaa ggacaacgcg gcgcgggccc gcgcgatccg cgccttgtgc gatctgcacg
     4261 ggcggctcgt cggcgacagc cgctactcga ccagcgatac gctcaaggaa tatcgctcgc
     4321 gcctctggag ccggctgacc aaggtcaaga ccgacctgaa gcgcgagttc ggccgcgacg
     4381 ccgacaagcg gcagtcgctc gaggacgccg cggtgctgga atcagccgac gcgctcagcg
     4441 cagttgccgc cgacagcctg gcggccagcc tgtcgctctc cggacagacg cagggcggcc
     4501 ccgcgtatct gctcggcggc ggcgcgggca ccggcgcgct gggcggcggc gctggcaccg
     4561 gggactatgg ccctcaactc gtcgcgctca tcgagcggac aatcaacccc gcattctggg
     4621 acaccgtcgg cggccccggc gcgatcgtct attaccagcc tctgcagtgc cttgtcgtgc
     4681 gggccacctc cgaaatccac ggcaacctcg gcgggctcct cggcggcctg cgagcggcgg
     4741 gaccgtagcc gacgctgcca gcgtcggccg acgacgcaag ctgcgtcgtc tacgttaggg
     4801 cgtgctcagc gtctcgttca cccgcg
//
LOCUS       DASRJB010000617        14451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_463573, whole genome shotgun sequence.
ACCESSION   DASRJB010000617 DASRJB010000000
VERSION     DASRJB010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14451
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463573"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..262)
                     /locus_tag="VFB80_16265"
     tRNA            complement(189..262)
                     /locus_tag="VFB80_16265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Arg,seq:ccg)
     gene            505..783
                     /locus_tag="VFB80_16270"
     CDS             505..783
                     /locus_tag="VFB80_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35386.1"
                     /translation="MWRAFFLAIGIYLCILGAECLVVDKFVMAAEKPVSALDPTAAPP
                     APATLFGGTTPAPLTMKKEIEPPEWAPWSLLSAGAVVILYSLTINRQG"
     gene            complement(822..1682)
                     /locus_tag="VFB80_16275"
     CDS             complement(822..1682)
                     /locus_tag="VFB80_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35387.1"
                     /translation="MTRQIASRFLLASVQGVLAAVVFCGPLGAADEPEKDKAVQEAQR
                     EQGLKNMQRSAAQYSLSSADTPERAFKFHETAALRFNNPITLTKDGALYVWTNHGRPQ
                     AVVKFFTFDNKTYTHAWLSLSEDNFVAERDGQVIWSPTEPGIKWREIQGAPKPAETAV
                     ERLRQMKTLSAKFSATYTATHLRAKPFELRLLTQPLLRYETDDDYRADGALFGYVQST
                     SPVGLLLLESRPTPDGYRWHYAYASLVTGPLTARYGDQEVFSLQRERDYAGGPKKPFR
                     MFQSLPVPKE"
     gene            complement(1760..1960)
                     /locus_tag="VFB80_16280"
     CDS             complement(1760..1960)
                     /locus_tag="VFB80_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35388.1"
                     /translation="MRFRLRTLLILLAVLPPLLAVGWWKYSAWKAEQERRAALSRTKL
                     LAPLIIDIIDVVWVDDLADPRK"
     gene            complement(2116..3558)
                     /locus_tag="VFB80_16285"
     CDS             complement(2116..3558)
                     /locus_tag="VFB80_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HZN35389.1"
                     /translation="MSIHRPLILSLLALLALTAGGRSSDFSDRLSAWQGGASAAPTPA
                     APPISAAPRMEAAPAPAAPAPRPSNAPRAYAPEPSGHLSGPTTCGQGPSFIGPTCDGV
                     AGCCGPAGCGGQGGHFHGGFCHGGSDCCDYSAWCCHPLVWARFDVLLWWRQGRDYPPL
                     VTTDPVNEPSTTAGILPDATILFGGDRVASNMAVGGRFDIGTWTDPRQCWGIGWRFFG
                     IGQDSTDFSINSLQNPVLAIPFTDANSDTNDALLIAYPGLRTGSIGVRGSSEVLGNDV
                     YGRFLLCRDCDSRLDFITGWNYTRVNDSVTIRSSATVTEIGGNIPVGTVTDIRDDFRA
                     HNEFNGGILGLLWERNCGCWSTQLMGRMSLGSNFEETSIRGSTQIASPGLPAQTDDQG
                     LFAADTNSGVFSRSEFTAITEIGLRLAYRCNNCTQLTVGYTFMYWNDIATASDAIDTF
                     IGSEAQRPRFAFEHSDFWVQGISLGFSREF"
     gene            complement(3690..4607)
                     /locus_tag="VFB80_16290"
     CDS             complement(3690..4607)
                     /locus_tag="VFB80_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZN35390.1"
                     /translation="MAASAELLAQHAALTQSVGIADLTGRTLLEIRGADRVNFLHAFC
                     TNNVKALQTGQSCEAFVTSPQGKTLGHVLVLCEADRLLLDTSPGQAKPLIDHFDRYVI
                     SEDVQFVDRTAETGVLLVAGARAAELLPIAGQQRAARVPYVSNGWFFWVAAADVQAMI
                     GKLEQAGALLCGPEAVEAARIEAGFPLFARDFTEDNLPQEVGRDAEAISFTKGCYLGQ
                     ETVARIDALGHVNKKLVGIKFAGDGVPEIGTPLLAGDKQVGSVTSSAWSPRLSAPLAL
                     AYIRTPHARAGSQLASPLGPAEVVALPLA"
     gene            complement(4616..4969)
                     /locus_tag="VFB80_16295"
     CDS             complement(4616..4969)
                     /locus_tag="VFB80_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN35391.1"
                     /translation="MELLVAHHEGYVLAKVNGPLDDTARGPFREKLHPLFAKKNTWLI
                     VDLSAAQRINSSGIGNLVALTADANTNGSRVTFVSLTPFVLSVMQVTKLDTFFDIAAS
                     LEEAVARPIRPAPAD"
     gene            complement(5054..6160)
                     /locus_tag="VFB80_16300"
     CDS             complement(5054..6160)
                     /locus_tag="VFB80_16300"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HZN35392.1"
                     /translation="MRGISRREALAISGAAAVGLGLADCTAAQEPRPAEELGRKRIDE
                     YDAKNTKVGHIVAATIRDDDIKFLQQIGLRSILVNFRPGESSLEEMRQVQERFARHQI
                     QIFGGLNYTYRSLKIQLGQPGRDEDIKQYQAFLRNLGKLGIGVATYDFHPANTYTTAT
                     VTSRGYTSREFSVDDFRAKVEKQRFEREYPADEIWANYSYFMKAVLPVAEEAGVTLAL
                     HPDDPPLAKMNGVAKLFTHVGGYHRAEEIAAGSRHWGLTFCVGTWSEGGDRMGKDVFG
                     MIRDFGRRGKIHVVHFRNVSSPLPRFHETFPDDGYLDMYQVMKTLREVGFTGCAVPDH
                     IPRLAGDDPLQRAGLAYCIASMRGMLRRANEEAG"
     gene            complement(6181..7155)
                     /locus_tag="VFB80_16305"
     CDS             complement(6181..7155)
                     /locus_tag="VFB80_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZN35393.1"
                     /translation="MPRSVITRPVLVILLGVAGAAPAEDAIDFRPVAGFLQLPEGMKL
                     GPCSGVDIDSRGNVYVIQRQVPPILCFDASGKLLRSWGTPLIGRDTDMQGAHGIRIDK
                     EEFVWITDRARHLVRKFDPAGHLLLTLGTEGSPGTGTNQFNRPADVAFGPSGEIYVAD
                     GYGNSRVMKFDARGKFLKTWGDKGSAAGQFDLPHTIAVGPDGRVYVGDRYNSRIQIFD
                     GEGNWKETWSGFVPCGIAFDRSGHLFVADGVSKVLLIDGRGRIVKSWGTEAEVLGLKP
                     GQRTVPPIANPGGWRFVPHLLAADGQGNLYLADVPNQMLHKLERIASK"
     gene            7367..9421
                     /locus_tag="VFB80_16310"
     CDS             7367..9421
                     /locus_tag="VFB80_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35394.1"
                     /translation="MKLCFQCLLLAAAGICLAATAAAQTTQSGSHPPMRPLPVAAKRE
                     LAPGKKVFVDAARGDDAAAGSEQAPWKTLAHATRQLQPGDTLYLRGGIYYERAQLRNS
                     GTEAAPITIAGYPGELAILDGGRREFFENPATSWQPLEGGVEGEYVSTRTYFDADDRQ
                     VPHQFLPAAWEPMWGIEEERPLALGNFGDSLVPLHGYRLAVDLRSKNELWIGGKGEMR
                     DTGIYCGPGLWFNRRTGRIHIRLARHELAGLGQRAYRGETDPRKLPLIIAVGFGGDVL
                     RASGIQHVRLRDLVLRGATGSPLIHVYGSQNVELDHVTAIGGFPALLINAAKNVRVTH
                     SAFRGLAAPWTSRAHMKYRGTASYQVVLQNNQPANENIELAWCEFTDDHDFAFLRFAT
                     NLSFHHNLVENFNDDGLECGPKLRAHTLFISQNRIGPCLIPLTQHEIEKDESPLDHDA
                     SKGVFVCRNVFDMRGGTYKGPPPAPDPSGAFLHEEGHLVGDHGGPTWPVMHVYHNTLL
                     RDTPVFRDYFLFGLGAQGLRHSERDVFNNIFVQSGKLPGVGFAGIQEAGRVREAGNIL
                     WGLEGGPELKADPFAKFRASPLFAKSREIQTGGWTADDRAVDPRLVRLKADRAQACDL
                     RLADGSPAIDTGVAVPAQWPDPLREQDQGPPDIGALPRGAAAWGVGVDGRIPLFGEPA
                     AG"
     gene            9567..11300
                     /locus_tag="VFB80_16315"
     CDS             9567..11300
                     /locus_tag="VFB80_16315"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HZN35395.1"
                     /translation="MNRRLECLGCLLLVLIWTVGKARAAEKSAGELLPASVVAYVEVP
                     QPAKVLDVVFDHPLARQLQQQPEYKAAQQTPQYEQLQAAIQKAEGALGQPWRKAAAEI
                     GAGGLFFGVDLTTRGVVALAAASSEAQAAKGRDAILELSRASAASIKENEHRGVKVHE
                     IGDAGFAAVGNWLVASNKRALVWQVIDNHQGHSEPLAGDEQFQSVLKARTGQPAAWLY
                     ADLRLLKLIPGFRQAIDKKSDNPPVELLAGGIIGSIPDAPYVTAALDLNPAGVKLKVA
                     LPCKPNETAKKREFYLGGEARGVAPPLLRPQRTLLSAATYRDFASLWRHAPDLFDDGI
                     NAKFAEAESNLTTLFAGRNFREDILGNLHPGMRLVVTRQEFPPDGITPAIKLPAGAIV
                     VRMKSPEETAKIFKITFQSVVGFLNVTGGMNGIDPLDLNSEKVGSALIVSGEYLPPRD
                     AAKRKEAPLHYNASPAAAFVGDLFILSSSRALAVELAEQVQREAPASAGVNSSLVLDA
                     AVGQAALADNRGPLIAQNMLEKGHDRAAAEQEIDRVLALLKNLQRSSLQLAVKEAGLE
                     LTLEVLLASQP"
     gene            11311..12636
                     /locus_tag="VFB80_16320"
     CDS             11311..12636
                     /locus_tag="VFB80_16320"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZN35396.1"
                     /translation="MPRNLAELTPQTAWLPLEAAPAFDRRMAAHLFRRAGFCASRREL
                     DEAVAAGLTATVKRLLTAGERSSAFDEEMRKFAEVALAAGNPQSLAGWWLHRLRHTPA
                     PLVEKMTLFWHGHFATSAAKVRDARLLYDQNELFRRHALGNFRPLVRGIARDPAMLLY
                     LDSATNRKNHPNENFAREVMELFCLGIGRYSERDIRELARCFTGWEILHGKFTFNTYQ
                     HDYGTKSLLGQSGPFDGDDGLRVILEQPACAEFLCGKLVRYFVTDEDLPAEWIAPLAQ
                     QFRESDLAIAPVLETIFTSRLFYSERAIGQKVRGPVELGVGLLRALEINTNMIQLAER
                     LRELGQLVFFPPNVKGWPGGKNWIDSSALLGRANLVRALADNIPPRSAAGPASGEQID
                     QLAELLLAVPPPAEVRAGLIAQLEQAGGMPGALRQAVSVLGALPEFQLS"
     gene            12647..13894
                     /locus_tag="VFB80_16325"
     CDS             12647..13894
                     /locus_tag="VFB80_16325"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35397.1"
                     /translation="MQNASRRKFLASISAAASAGLVSLAPRAPGFLLESAALGADRPA
                     GDRILVVVQLSGGNDGLNTVIPYGDEIYRKKRPSLALGADNVIKLERDLGLHPRMKGA
                     ETLLEKRWLAVVQGVGYPNPNRSHFESMDIWHTAHTQPQDRAAGWLGRSFDAQRAAGR
                     GSADPPGMHLGAESQPLALAARDVPSPSIRSLDQFQLRVDGDAQRASAIQAAASAPRR
                     QASELLKFVQTRTTAALEISRRITAANQGYKTPVAYPETELAGKLKQVAQLIDAGLST
                     RVYYVALDGFDTHSDQANAHAGLLDQLAGGLAAFAEDLQNHGHLDRVVTLAFSEFGRR
                     VEENASRGTDHGAAAPVFLVGSKVKPGLIGQHPKLDDLADGDIKFHTDFRCVYAALLA
                     KWLGWPVEPILGEGFAPAAVLQA"
     gene            13905..>14451
                     /locus_tag="VFB80_16330"
     CDS             13905..>14451
                     /locus_tag="VFB80_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN35398.1"
                     /translation="MLRALLLLVVCLIWVSPVQGQTPSEQVRLAGIVLKWLRTDKQAN
                     YDRAAALIEQAAKGGAKIVCTTECFLDGYAIKDKSIPLETYRALGEPIPDGAYFQKLA
                     QLADRLDIWLVAGMLEADGEQRFNTAVLIDAEGKLAGRYRKHKLEHELVRNTPGDQTP
                     HFDTPYGRLGLMICADRRDSDL"
BASE COUNT         2478 a         4722 c         4815 g         2436 t
ORIGIN      
        1 acgaccgcct ggcccgtggc ctagtggtgc cgatacttgg gcgtgcgatt gataagcttt
       61 ggcatgtggg cttctcccgt ggttgaagcc caaaacggta gtttaccgtt ttgggtttgg
      121 ggaaaacgcc gcactgccac acgcgggcag gtatcgtaac tctagattcg gctgtgactt
      181 accgagaata tccccgacag ggctcgaacc tgtaaccttc ggcttcggag gccgacgctc
      241 tatccaattg agctacgggg acttctgagc tgcggggact tccagacggg tgaggaattg
      301 taacagtgca cgtggtggcc agcaatgact cggctggtga cgattgcagc gcaagtgacg
      361 cctctgccgc agtcgggaaa gcaactgcgg ctgacgacaa gcggccgccg atgtgagaag
      421 agaacggact cgccggcggg tgattctctg cacctgctgc ggcctgcaac ggactgcccc
      481 acgaagtcga agggctggca ccgcatgtgg cgagcgttct ttctggcgat tggcatctac
      541 ctgtgcattc tcggcgcgga atgcctcgtg gtcgataagt tcgtgatggc cgccgagaag
      601 ccggtctccg cgctcgaccc caccgccgcc ccgccggccc cagcaactct gtttggcggc
      661 accacacccg cgccgctcac catgaagaaa gagatcgagc cgcccgagtg ggccccgtgg
      721 agcctgctct ccgccggcgc cgtcgtgatc ttgtattcgc tgacgatcaa ccggcaggga
      781 tagaggcccg ccggtcccct ccctttccct ccgagtcgca gtcactcttt cggaaccggc
      841 agcgactgga acatgcgaaa aggcttcttc gggcctccgg catagtcccg ctcccgctga
      901 agggaaaaga cttcctggtc tccgtaccgg gcggtcaacg gcccggtgac caggctggcg
      961 tatgcgtagt gccagcggta gccgtctggg gtcggacggg actctaacag aagtaacccg
     1021 accggcgacg tgctttgaac gtacccgaac agcgccccgt ccgcgcggta gtcgtcgtcc
     1081 gtttcgtagc ggaggagcgg ctgagtcagc agcctcaact cgaacggctt cgcgcgcaaa
     1141 tgcgtcgccg tatacgtcgc gctgaacttg gccgagagag tcttcatctg ccgcaggcgc
     1201 tccaccgccg tttccgcggg cttgggggcg ccctgaatct cacgccactt gatccccggt
     1261 tcggtcgggc tccaaatcac ctggccgtca cgctcggcca cgaagttgtc ttcggacagc
     1321 gacagccacg catgcgtata ggttttgttg tcgaaggtga agaacttcac gacggcttgc
     1381 gggcggccgt gattcgtcca cacgtagagg gcaccgtcct tcgtgagggt gatggggttg
     1441 ttgaaccgca aggcggcggt ctcgtggaac ttgaaggccc gctccggcgt gtcggccgat
     1501 gaaagggaat actgcgcggc tgaacgctgc atattcttga gcccctgctc ccgctgcgcc
     1561 tcctgcaccg ctttgtcttt ctccggctcg tcagccgcgc caagcgggcc acagaagacg
     1621 acggcagcca ggaccccctg aacagacgcc aagaggaagc ggctcgcgat ttggcgagtc
     1681 atgtcttcgc tactgtgtga agtgggacgt aaggaggttg cggacaatgt tcgattaatt
     1741 ggatctgctc ggccggacgc tacttccgcg ggtccgccag gtcgtccacc cagactacgt
     1801 caatgatgtc gatgatgagg ggtgccaaca gcttcgttcg cgacagcgcc gcccgccgtt
     1861 cctgctccgc cttccacgcc gaatacttcc accagcccac ggccaacagc ggcggcagga
     1921 cggccagcag gatcagcagc gtgcggaggc gaaagcgcat gggggcattc tatccgcggc
     1981 tggctatcga cgtgaaacac aaaggacaaa actacccacg gcgaaaggcg aagcggcacc
     2041 cttccgccag accttgcttg atcgagtcct gtcgtgcttc gtgtccggtc ctctctcggg
     2101 agaggatgcg agtggctaga actcgcggct gaagccgagg ctgatgcctt ggacccagaa
     2161 gtcgctgtgc tcgaacgcga accgcggccg ctgggcctcc gatccgatga acgtgtcgat
     2221 cgcgtcggac gccgtggcga tgtcgttcca gtacatgaac gtgtagccga cggtgagctg
     2281 cgtgcagttg ttgcagcgat aggccagccg caggccgatt tcggtgatcg cggtgaattc
     2341 gctccgcgag aaaacgccgc tgttcgtatc ggcggcgaac aagccctgat cgtcggtttg
     2401 ggcgggcaag cccgggctgg cgatttgcgt ggagccgcgg atgctcgttt cctcaaaatt
     2461 gctgcccagc gacatccggc ccatcagctg cgtgctccag cagccgcaat ttcgctccca
     2521 caagaggccc agaattccgc cgttgaattc gttgtgggcc cggaagtcgt cgcggatgtc
     2581 ggtcacggtg ccgacgggga tgttgccgcc gatctcagtc acggtggcgc tgctgcggat
     2641 cgtcacgctg tcgttgacgc gcgtgtaatt ccagccggtg atgaagtcga gccggctgtc
     2701 gcagtcgcgg cagagcagga aacggccgta gacgtcgttg cccagcacct ccgacgagcc
     2761 gcggacgccg atcgagcccg tccgcaggcc gggataggcg atgagcaggg cgtcgttggt
     2821 gtcgctgttg gcgtcggtga agggaatcgc cagcaccggg ttttgcagcg agttgatgct
     2881 gaagtcggtc gagtcctggc caatgccaaa gaaccgccag ccgatgcccc agcactgccg
     2941 gggatcggtc caggtgccga tgtcgaagcg gccgccgaca gccatgttgc tggccacgcg
     3001 gtcgccgcca aagaggatcg tggcgtcggg caggatgccg gccgtcgtcg aaggctcgtt
     3061 gaccggatcg gtggtgacca gcggcggata gtcgcggccc tgccgccacc acaggagcac
     3121 gtcgaaccgc gcccagacga gcggatggca acaccaggcg ctgtagtcgc agcaatcgct
     3181 gccgccgtgg caaaaaccgc cgtgaaaatg gccgccctgt ccgccgcacc cggccggccc
     3241 gcagcatccg gcgacgccgt cgcaggtcgg cccgatgaag ctgggcccct ggccacaggt
     3301 cgttgggccc gagaggtggc cgctcggctc aggcgcatac gccctcgggg cgttgctcgg
     3361 ccggggagct ggagccgctg gtgcaggagc ggcttccatc cgcggcgctg ccgaaattgg
     3421 cggagcggcg ggcgtcggcg cagcactcgc tccgccctgc caggcggaga gtcggtcgct
     3481 gaagtcggag ctgcggcctc cagcggttag cgccagaagc gccagcaagc tcagaatcag
     3541 cgggcgatga atgctcatga gcaagtgcag ttcgggagta cgagcagaat cgcgcacgag
     3601 gcggctgcaa ggctgtatcg aatatttcgg ttgcgccggc gtttccggtt gaggaaagcc
     3661 gcgaaggtgc gcagagtttt cgggcggcat tacgctagcg gcaacgccac gacctcagct
     3721 ggccccagcg gcgaagccag ctggctgccc gcgcgggcgt ggggcgtgcg gatataggcc
     3781 agtgctagcg gcgccgacaa tcgcggcgac caggcggacg acgtgacgct gccgacctgc
     3841 ttgtcgccag ccagtagcgg agttccgatt tccggcacgc catcgccagc gaatttaatt
     3901 ccaacgagct tcttattcac atgccccagc gcgtcgatgc gggccaccgt ttcctggccg
     3961 agatagcagc ccttggtaaa gctgatcgct tccgcatcgc ggccgacttc ctgcggaagg
     4021 ttgtcctcgg tgaagtcgcg cgcgaagagc gggaagccgg cttcgatccg cgcagcctcg
     4081 accgcctctg ggccgcagag cagagcgcca gcttgttcga gcttgccgat cattgcctgc
     4141 acgtccgccg cagcaaccca gaagaaccag ccgttactca cgtagggtac gcgggccgca
     4201 cgctgctggc cggcgatcgg cagcaattcc gccgcccgcg ctccggccac cagcagcacg
     4261 cccgtctcgg ccgtgcgatc gacgaactgt acgtcctccg agatgacata gcggtcgaaa
     4321 tggtcgatca gcggtttggc ctggccggga gaagtgtcca agagcagccg gtcggcttcg
     4381 cagagcacca gcacgtgccc cagcgtcttg ccctgcgggc tggtgacgaa tgcctcgcag
     4441 ctctggccgg tttggagcgc cttgacgttg ttcgtgcaga acgcgtgcag aaagttcacg
     4501 cggtccgcgc cgcgaatttc aagcagcgtg cggccggtca ggtcggcgat gccgacgctc
     4561 tgcgtcagcg cggcgtgttg cgcgagcagt tcagccgaag cagccatggg cggcctcaat
     4621 cggccggggc cgggcggatc gggcgggcca ccgcttcctc caggctcgcc gcgatgtcga
     4681 agaacgtgtc gagcttcgtc acctgcatca ccgacaacac gaacggagtc aacgagacga
     4741 acgtcacgcg gctgccgttg gtgttggcgt cggccgtcag ggccacgaga ttgccgatgc
     4801 ccgacgaatt gatccgctgc gctgcggaca aatcgacgat cagccaggtg ttctttttgg
     4861 cgaacagcgg gtgcagcttt tcgcgaaagg gcccgcgggc ggtgtcgtcc agcggcccat
     4921 tgaccttggc caggacataa ccctcgtggt gtgcgaccag caattccata tcattctcct
     4981 ctgtccgcca tcatagcgac tgctcgcccg gtcgccatgg cgggtcgccg ccccagtcgt
     5041 gagccggcat cgctcatcca gcttcctcgt tggcgcggcg cagcatgcca cgcatcgagg
     5101 cgatgcaata ggccaggccc gcgcgctgca gcggatcgtc gccggccagc ctggggatgt
     5161 ggtcgggcac cgcgcagccg gtgaagccga cttcgcgcag cgtcttcatg acctggtaca
     5221 tgtcgaggta gccatcgtcg gggaaggtct cgtgaaatcg cggcagcggc gagctgacat
     5281 tacggaagtg gaccacgtgg attttgccgc ggcggccgaa gtcgcgaatc atcccgaaaa
     5341 cgtctttgcc catgcggtct cctccttccg accaggtgcc gacgcagaag gtcaatcccc
     5401 agtgacggct gcccgccgcg atctcctcgg cccggtggta gccgccaacg tgtgtgaaca
     5461 gcttggccac gccgttcatc ttcgccagcg gcggatcgtc gggatgcagg gccagcgtga
     5521 cgccggcttc ctcggccacg ggcaacaccg ccttcatgaa atacgagtaa ttggcccaga
     5581 tttcgtcggc cgggtattcg cgctcgaacc gctgcttttc gaccttcgcg cggaagtcgt
     5641 ccacgctgaa ctcgcgcgac gtgtagccgc ggctggtcac ggtcgccgtg gtgtaggtgt
     5701 tcgccgggtg gaagtcgtac gtggcgacgc cgatgccgag cttgccgaga ttgcgcagga
     5761 atgcctggta ctgcttgata tcctcgtcgc ggcccggctg gcccagctga attttgagcg
     5821 agcggtacgt gtagttgagg ccgccgaaaa tctggatctg atgccgggcg aagcgctctt
     5881 gcacctgccg catctcttcg agcgacgatt cgccgggcct gaagttgacc aggatcgagc
     5941 gaaggccgat ttgctgaagg aacttgatgt cgtcgtcgcg gatggtggcc gcgacgatgt
     6001 ggcccacttt ggtgttcttg gcgtcgtact cgtcgatccg cttgcgtccc agctcttccg
     6061 cgggacgcgg ctcctgagcg gcagtgcagt cggcgagccc caacccgacg gctgccgcgc
     6121 cggaaatcgc cagagcctca cggcgggaaa tgcctcgcat cgttttgcct ccagggggcc
     6181 ctatttgctc gcaatccgct ccagcttgtg caacatctgg ttggggacat cggccaggta
     6241 caggttgccc tggccgtcag ccgccagcag gtgcggcaca aatcgccaac ctccggggtt
     6301 ggcaatcggc gggacggttc tttgccccgg tttcagtccg agtacttccg cctcggttcc
     6361 ccaggacttc acgatccggc cgcgtccgtc gatcaacagc accttgctca ctccgtcggc
     6421 gacgaacaag tggcccgagc ggtcgaatgc aatcccgcac ggcacaaacc cgctccaggt
     6481 ctccttccag ttgccttcgc catcgaaaat ctgaatgcgc gagttgtaac ggtcgcccac
     6541 atagacccgc ccatccggac cgaccgcgat ggtatgcggc agatcgaact ggcccgccgc
     6601 cgatcccttg tcgccccagg tcttgaggaa tttgccgcgg gcgtcgaact tcatcacgcg
     6661 gctgttgccg tagccgtcgg cgacataaat ctctcccgac ggtccgaagg ccacgtccgc
     6721 cggccggttg aattgattcg ttccggtgcc gggacttccc tcggtgccga gcgtgagcag
     6781 caggtggccc gcggggtcga acttgcggac cagatgccgg gcgcgatccg tgatccagac
     6841 gaattcttcc ttgtcgatgc ggatgccgtg agcgccctgc atatccgtgt cgcggccgat
     6901 cagcggcgtc ccccaggagc gcagcagctt gccggaggca tcgaagcaca ggatgggcgg
     6961 aacctgccgc tgaatgacgt agacgttgcc gcggctgtcg atgtccaccc cgctgcacgg
     7021 ccctagcttc attccttccg gcagctggag aaagccggcg accggccgga agtcgatcgc
     7081 atcttccgcc ggcgcagcgc ccgcgactcc gagcaggatg acgaggaccg gccgcgtgat
     7141 tacgcttcga ggcatggcaa tcctcttcaa gtaggcccct tctgccggaa ggggcctgcg
     7201 ggaagcgcag cgaccagtcc cgcccggccg gcgggaccta caaagtagag gttgtcggcg
     7261 tgcgctcaga acctagccca acaaggcgcg tccagcaacc ggccgagttg acgttggaca
     7321 acaagccacg tgacaatccg agggatctgg tattggagac tctgcaatga aactctgctt
     7381 ccaatgtctg ctgctggccg ctgccggcat ttgcctggcg gccactgccg ctgcccagac
     7441 gacgcaaagc ggctcgcatc cgccgatgcg gccgctgccg gtcgcggcca agcgcgagct
     7501 ggcgccgggg aagaaagtat tcgtcgatgc ggcccgcggc gatgatgcgg cggcgggcag
     7561 cgaacaagcg ccgtggaaga cgctcgcgca tgccacgcgg caactgcagc cgggcgatac
     7621 gctgtacctg cggggcggca tctattacga gcgcgcccag ctgcggaatt ccggcactga
     7681 ggccgcgccg atcacgatcg ccggctatcc gggcgagctc gccatcctgg acggcggccg
     7741 ccgcgagttt tttgagaatc ccgcgacgag ctggcagccg ctggagggag gcgtggaggg
     7801 cgaatacgtc tcgaccagga cgtacttcga cgccgacgac cggcaagtgc cgcaccagtt
     7861 cctgcctgcg gcctgggagc cgatgtgggg gatcgaggag gagcggccgc tggccctggg
     7921 gaactttggc gattcgctgg tgcccctgca cggctatcgc ctggcggtcg atctgcggtc
     7981 gaagaacgag ctgtggatcg gcggcaaagg cgagatgcgc gacaccggca tctactgcgg
     8041 gccgggcctc tggttcaatc gccggacggg ccgcattcac atccgcctgg cccgtcacga
     8101 gctggcagga ttgggccagc gggcctatcg cggcgaaacc gatccgcgga agctgccgct
     8161 catcatcgcg gtcggctttg gcggcgacgt gctccgcgcc agcggcattc aacacgtgcg
     8221 ccttcgcgac ctcgtgctcc gcggggctac cggcagcccg ctgattcacg tttacggttc
     8281 gcagaatgtc gaactcgatc atgtcacggc gatcggcggc tttcccgcgc tgctcatcaa
     8341 cgcggccaag aacgtccgcg tgacccactc ggcgtttcgc gggctggctg cgccctggac
     8401 gtcgcgggcc cacatgaaat accgcggcac tgcgtcgtac caggtcgtcc tgcagaacaa
     8461 tcagccggcg aacgagaaca tcgagctggc ctggtgcgaa ttcaccgacg accacgactt
     8521 tgcgttcctg cgattcgcga cgaacctgtc gttccaccac aacctcgtcg agaacttcaa
     8581 cgacgacggc ctggagtgcg ggcccaagct ccgcgcgcac acgctgttca tctcgcagaa
     8641 ccggatcggg ccctgcctga tcccgctgac gcagcacgag atcgagaagg acgaatcgcc
     8701 gctcgaccac gacgccagca agggcgtgtt cgtgtgccgc aacgtctttg acatgcgggg
     8761 cggcacgtac aaaggtccgc cgcccgcgcc ggatccgagc ggcgcgttcc tgcacgaaga
     8821 gggacacctg gtcggcgacc acggcgggcc gacctggccc gtgatgcacg tctatcacaa
     8881 cacgctgctc cgcgacacgc ccgtctttcg cgactacttc ctcttcggcc tgggcgcgca
     8941 gggcctgcgg cattcggagc gcgacgtctt caacaacatc ttcgtgcagt ccggcaagct
     9001 gccgggagtc ggcttcgccg gcattcaaga ggccggccgc gtccgcgaag cgggcaacat
     9061 cctctggggc ctggagggcg gcccggagct caaggccgat cccttcgcaa agttccgggc
     9121 ctcgccgctg tttgccaaga gccgcgagat tcagaccggg ggctggacgg ccgacgaccg
     9181 ggccgtcgat ccgcggctcg tccggctgaa ggcggaccgc gctcaagcct gcgatctgcg
     9241 gctggccgac ggcagcccgg cgatcgacac aggagtcgcc gttccggccc agtggcccga
     9301 tccgctgcgc gagcaagacc agggcccgcc cgacatcggt gcgctgcccc gcggcgcggc
     9361 ggcctggggc gtcggcgtcg atggccggat tccgctgttt ggagagcctg ccgccgggtg
     9421 aaccgtgcaa ttgaaaccgc tgggggctat gttgggtacg tcaacatagt agtcatctcg
     9481 ctccgcgaga tgtttgcctc acgcggagtg tgaggtcaac tatgtccggt cggatggcag
     9541 cacacgacct tctgcggaga gccctcatga atcgccgttt ggagtgcctg ggctgtctgc
     9601 tgctggttct gatctggacc gtgggaaagg ctcgggcggc ggagaagtcc gctggcgagt
     9661 tgctgcccgc ctctgtggtg gcctacgtcg aggttccgca gccggcaaag gtgctcgacg
     9721 tcgtgttcga tcatccgctg gccaggcagt tgcagcagca gcccgagtac aaggcggcgc
     9781 agcaaactcc gcagtacgag cagctgcagg cggccatcca gaaggcagag ggcgcgctcg
     9841 gccagccctg gcggaaggcc gccgccgaaa tcggcgcggg cggactgttt ttcggcgtcg
     9901 atctgaccac gcggggggtc gtcgccttgg ccgcggccag ctcggaggcc caggcggcca
     9961 aggggcgcga cgcgattctg gaactgtcgc gggctagcgc cgcctccatc aaggagaacg
    10021 agcatcgcgg cgtcaaggtt cacgaaatcg gcgacgcggg cttcgctgcc gtcggcaact
    10081 ggctcgtcgc ctccaacaaa cgggccctgg tctggcaggt gatcgacaac caccagggcc
    10141 acagcgagcc gctcgccggc gacgagcagt tccagtcggt gctcaaggcc cgcaccgggc
    10201 agcccgcggc ctggctgtac gccgacctgc ggctgctgaa gctcattccc ggtttccggc
    10261 aggcgattga caagaaaagc gacaatccgc ctgtcgagct gctggccggc ggaatcatcg
    10321 ggtcgattcc cgatgcgccc tatgtcacgg cggcactgga tctgaatccg gcgggcgtca
    10381 aactgaaggt ggcgctgcca tgcaagccga acgagaccgc gaagaagcgc gagttctacc
    10441 tgggcggcga ggcccgcggc gtcgctccac cgctcttaag gccgcagcga acgctgctgt
    10501 cggcggccac ctatcgcgat ttcgcctcgc tctggcggca cgcgcccgac ttgttcgacg
    10561 acggcatcaa cgccaaattc gcggaggccg aaagcaactt gaccacgctc ttcgccggcc
    10621 gcaacttccg cgaggacatt ctgggaaatc tgcatccggg gatgcggctg gtggtgacgc
    10681 ggcaagagtt ccccccggac ggcatcacgc cggcaatcaa gctgccggcg ggggcgatcg
    10741 tggtgcggat gaaatcgccg gaggagacgg ccaagatctt caagatcacg tttcagagcg
    10801 tggtcggctt cctgaatgtg accggcggga tgaacgggat cgacccgctg gacctgaata
    10861 gcgagaaagt cggctcggcc ttgatcgtgt cgggcgaata cctgccgccc agggacgcgg
    10921 ccaagcggaa ggaagcgccg ctgcactaca acgcctcgcc ggcggcggcg tttgtcggcg
    10981 atctgttcat cctctccagc agccgggcgc tggcggtgga gctggcggag caagtccagc
    11041 gcgaagcgcc ggcatcggcc ggcgtgaaca gcagcctggt gctcgacgcc gcggtgggcc
    11101 aggcggccct ggccgacaat cgcggcccgc tgatcgccca gaacatgctg gagaaggggc
    11161 acgaccgcgc cgcggccgag caggagatcg atcgcgtgct ggcactgctg aagaatctgc
    11221 agcggtcgtc gctgcagcta gccgtcaagg aagccggcct ggaactgacg ctggaagttc
    11281 tgctggcgag ccaaccctga ggccgctcgt atgccacgca acctggccga gctgacgccg
    11341 caaaccgcct ggctgccgct ggaggcggcc ccggcctttg accggcggat ggcggcccac
    11401 ctgtttcgcc gcgcgggctt ttgcgcctcg cggcgcgagc tggacgaggc ggtcgccgct
    11461 ggcctgacgg cgacggtcaa gcgactgctt acggcaggcg agcgaagcag cgccttcgac
    11521 gaagaaatgc ggaaattcgc cgaggtcgcc ctcgccgccg gcaatccgca aagtctcgct
    11581 ggctggtggc tgcaccgcct gcggcacacg cccgcgccgc tcgtggagaa gatgaccctg
    11641 ttctggcacg ggcacttcgc caccagcgcc gccaaggtgc gcgacgcgcg tctgctttac
    11701 gatcagaacg aactgttccg ccgccacgcc ctgggcaatt ttcgcccact ggtccgcggg
    11761 atcgcccgcg acccggcgat gctgctctat ctcgactccg cgaccaaccg caagaaccat
    11821 cccaacgaga atttcgcccg cgaagtgatg gagctcttct gcctgggcat cggccgctac
    11881 agcgaacgcg acatccgcga gctggcccgc tgcttcaccg gctgggaaat cctccacggc
    11941 aagttcacgt tcaacaccta tcagcacgat tacgggacga agtcgctctt gggccagagc
    12001 ggcccattcg atggcgacga cggcctgcgg gtgatcctcg agcagccggc gtgcgccgag
    12061 ttcctctgcg gcaagctcgt gcggtatttc gtcaccgacg aggacctgcc cgccgaatgg
    12121 atcgcgcccc tggcccagca gtttcgcgag agcgacctgg ccatcgctcc ggtcctggaa
    12181 acgatcttca ccagccggct gttctattcc gagcgggcca tcggccagaa ggtccgcggg
    12241 ccggtcgagc tgggcgtggg cctgctgcgg gcgctggaaa tcaacacgaa catgatccag
    12301 ctagccgagc ggctgcggga gctggggcag ctcgtgttct ttccgccgaa cgtcaagggc
    12361 tggcccggcg gcaagaactg gatcgattcc tcggcgctgc tgggccgggc gaacctggtg
    12421 cgggccctgg cggacaacat tccgccgcga agcgccgccg ggccagcgag cggcgagcag
    12481 attgatcaac ttgccgagct gctcttggcc gtgccgccgc ccgcggaagt gcgcgccggg
    12541 ctgatcgccc agctcgagca ggccgggggc atgccggggg cgctgcggca ggccgtgagc
    12601 gtgctcggtg cactgccgga gtttcaactg tcatagggag gccgccgtgc aaaacgcatc
    12661 gcgccgcaag tttctcgcgt cgatttcggc agcggcctca gcggggctgg tttcgctggc
    12721 gccgcgcgcc ccggggttct tgctcgaatc ggcggcactt ggcgccgatc gcccggcggg
    12781 cgatcggatt ctggtcgtcg tgcagctcag cggcggcaac gacgggctga acaccgtcat
    12841 tccgtatggc gacgaaatct atcgcaagaa gcggcccagc ctggccttgg gcgccgacaa
    12901 cgtgatcaag ctagagcgcg acctggggct gcacccgcgg atgaaggggg cggaaacgct
    12961 gctcgagaag cgctggctcg cggtcgtgca gggcgtgggc tatcccaacc ccaaccgctc
    13021 gcacttcgag tcgatggaca tctggcacac cgcccatacg cagccgcagg accgcgccgc
    13081 gggctggctg ggccgctcgt tcgacgccca gcgcgccgcc ggtcgcggca gcgccgatcc
    13141 gccgggcatg caccttggcg ccgagtcaca gccgctggcg ctggcggccc gcgacgtgcc
    13201 ctcgccgtcg atccgctcgc tcgatcagtt ccagctccgg gtggatggcg acgcccagcg
    13261 ggccagcgca atccaggccg ctgctagcgc accgcggcgg caagccagcg agctgctcaa
    13321 gttcgtgcag acgcgaacga ccgcggccct ggaaatcagc cggcggatca cggctgcgaa
    13381 ccaaggctac aaaacgcccg tggcctatcc cgaaacggag ctggcgggca agctgaagca
    13441 ggtggctcag ctgatcgacg ccgggctttc cacgcgcgtc tattacgtcg cgctcgacgg
    13501 gttcgacacg cactccgacc aggccaatgc ccatgcgggc ctgctcgacc agctggccgg
    13561 cgggctggcg gcatttgccg aagacttgca aaaccacgga cacctggacc gcgtcgtgac
    13621 gctggccttc agcgaattcg gccgccgcgt ggaagagaac gccagccgcg gcacggatca
    13681 cggcgcggcg gccccggttt tcctggtcgg cagcaaggtc aagccgggcc tgatcggtca
    13741 gcatcccaag ctggacgacc tggccgacgg cgacatcaag ttccacaccg acttccgctg
    13801 cgtctatgcc gcgctgctcg cgaagtggct cggctggccg gtcgagccga tcctcggtga
    13861 aggctttgct cccgcggccg tgctgcaagc gtaggatcca gaacatgctt cgcgctctct
    13921 tgcttctcgt tgtgtgcttg atctgggtgt cgccggtgca aggccaaacg ccgagcgagc
    13981 aagtgcggct ggcgggcatc gtgctgaaat ggctgcggac cgacaagcag gccaactacg
    14041 atcgcgcggc ggcgctgatc gagcaggcgg ccaagggagg cgcaaaaatc gtctgcacga
    14101 ccgagtgctt cctcgacggc tatgccatca aggacaagtc gatcccgctc gaaacgtacc
    14161 gggccctcgg cgagccgatt ccggacgggg cctatttcca aaaactcgcg cagctggccg
    14221 accggctcga catctggctc gtggctggca tgctggaggc cgacggcgag cagcgcttca
    14281 acacggccgt gctgatcgac gcggagggca agctcgccgg gcgctatcgc aagcacaagc
    14341 tggagcacga actggtccgc aacacgccgg gcgaccagac gccgcacttc gacacgccct
    14401 atggccgcct cgggctgatg atctgcgccg accgccgcga cagcgacctg g
//
LOCUS       DASRJB010000618         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1027978, whole genome shotgun sequence.
ACCESSION   DASRJB010000618 DASRJB010000000
VERSION     DASRJB010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027978"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..829)
                     /locus_tag="VFB80_16335"
     CDS             complement(155..829)
                     /locus_tag="VFB80_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZN35399.1"
                     /translation="MFAPVRYLAAVAALLLWAQAADSRPILSGKVVGVTDGDTLTLLT
                     ADKRQVKIRPDGIDAPESGQPFGTKAKQALSAKVFGKAVTVEDRGPDKYGRTIGRVKN
                     LGISVNQAMVMDGWAWHFTKYSDDETLATAEKAARGAKRGLWADPSPIPRWDWRDGER
                     GGVATLPITGAADPAAATLTVYLTKTGEKYHAAGCRHLSKSRIPISLAEAKRRYTPCS
                     VCSSPR"
     gene            1283..1939
                     /locus_tag="VFB80_16340"
     CDS             1283..1939
                     /locus_tag="VFB80_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuvB-like domain-containing protein"
                     /protein_id="HZN35400.1"
                     /translation="MQTIQLFEQHRPRQWSEVVGQQKALAVLDRLRQRGGLAGRAYFL
                     SGATGTGKTTIALLIAAEVADDFCVTSIDDGAQVNAETLAEMGRDKQRRPFGRGCCWI
                     INEAHKLSNAAITRLLGICEQMPPWFTVIFTTTSEAKEGLFDAKLDSHPLLSRCNELP
                     LARRDLAKAFAERARMIAQVEGLDGQPLDRYIALAKDCRNNLREMLTRIENGEMLAAS
                     "
     gene            2059..2748
                     /locus_tag="VFB80_16345"
     CDS             2059..2748
                     /locus_tag="VFB80_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HZN35401.1"
                     /translation="MAHLFSTGLFFGQRAWHGLGTTLPADSPARLSIDDSVRLAGLDW
                     QVETRPIFLEGGQQITGHQAVIRTDSTKVLGVVGDRYQPLQNADHFRWFAPFLDTREV
                     AFETCGALRDDQLVWVLAKIQRPDAVVSASDKIAKYLLLSSSHDGSAATSVGYCPIRV
                     VCWNTLSTGLSHQASKLFKVRHTRSQAETLAAIRETINLADETFEATAANIASCWPAR
                     FLPPICGATSS"
     gene            2727..3002
                     /locus_tag="VFB80_16350"
     CDS             2727..3002
                     /locus_tag="VFB80_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35402.1"
                     /translation="MRRYVKLVLELPEDEAAYSGRARNSLADVVELARRGVGNDGKTV
                     WSAYNGVTHYVTHAYGHSADSRLRANWYGEGRRMNDRALTLALQVAG"
     gene            3123..3476
                     /locus_tag="VFB80_16355"
     CDS             3123..3476
                     /locus_tag="VFB80_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35403.1"
                     /translation="MQSHVLIEVRGGVAEYQTAGPVAVTLIDYDNDPTAEFPHDWDNV
                     DPAATSRPVYYLSQLDPAERNVLRNTLAPGHDDLLAACEAVLESLERNLAADYLPETR
                     AALADAIAKAKGGGQ"
     gene            3531..4085
                     /locus_tag="VFB80_16360"
     CDS             3531..4085
                     /locus_tag="VFB80_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35404.1"
                     /translation="MSLLACEGDDQWEVALDVEELELAEEGEDIDLDDFHRFRGELYR
                     LLAQLDQPDLAWPSAREIELQSPPACRRRNTLPRSCAIPLEERAEKGDDAAMATKELC
                     DKYGKNSALSINAAIRAELPGGSELLDALEIAHKAAGRRDDPAAATVHRILESLRAAK
                     AALEAEIRRRYDMPPRDERGEPQG"
     gene            complement(4332..>4988)
                     /locus_tag="VFB80_16365"
     CDS             complement(4332..>4988)
                     /locus_tag="VFB80_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZN35405.1"
                     /translation="KAMILLGINCGFGNSDCGTLPLAALDLTGGWVNYHRPKTGIDHR
                     CPLWPETVKAIKAALAKRPTPKSDDAEATVFVTKYGASWAGSAVHNPVSREFGKLAKE
                     QDVARTFYDLRHTFRTVADECRDQPAVNSIMGHADASMAATYRERIADDRLRAASDHV
                     RRWLYPEVVAKEKAEREAAEADAEPRKAASRQAKSGARGKAQPTAESTTGGFALRIVG
                     "
BASE COUNT          871 a         1620 c         1591 g          906 t
ORIGIN      
        1 tctttcccga gcgaacgacc gccgtcgacg cagcgggctg cggccaacga gattcccgcg
       61 attaacgtcg cgagcatcaa gtcgcggatc aacagcggag acgccgcgac cggaggggcg
      121 tcccgcctga cgaggcgcca gcctacccaa ccggctaccg cggcgacgaa cagacggagc
      181 agggcgtata gcggcgcttc gcctccgcga gcgagattgg gatgcggctt ttgctgagat
      241 gccggcagcc ggcggcgtga tacttctcgc ctgtcttcgt caagtacacc gttagcgtcg
      301 cggcggccgg gtccgcagcg ccagtgatcg gcagagttgc cactccgccc cgctcaccgt
      361 cccgccaatc ccaacggggg atcggcgacg gatcagccca aagtccgcgc ttggccccac
      421 gagctgcttt ctctgcggtg gcgagcgtct cgtcatcgct gtacttcgtg aagtgccagg
      481 cccacccgtc catcaccatc gcctggttga cgctgatacc aaggttcttc acgcgcccta
      541 tcgtccggcc gtacttgtcc ggtccacggt cctcaaccgt gactgccttc ccgaacacct
      601 tggctgagag cgcttgcttg gcctttgtcc caaacggctg accgctctcc ggagcgtcga
      661 ttccgtcagg cctaatcttg acctgccgct tgtccgcggt caggagcgtg agcgtgtcgc
      721 cgtctgtgac gcccacgacc ttgccggaga ggatcggccg agagtccgcg gcctgagccc
      781 acagtaggag ggcggcgacg gcggctagat agcggacggg tgcgaacatc gggggccagt
      841 atccgcggca ggtaatcggg agtccagcag aatcggctcc aatcctagta gagtgcgttt
      901 gtggtggatc tgggttaacc gcgggagtcc cagcgcgaat cgggtagggg gtcccggtta
      961 caattctgct ttgctggggt cccccgcggt catctctcga ctagcagcgt tgccgcgcaa
     1021 acggtctgca aacggcggcg aaacggcgtt caaactatga cgcaacaagc aggcgtcgca
     1081 gttttttgaa cttgcggcga gcgacgacaa aatccgttcg catctatccg cttgccgcgg
     1141 cggcacttaa ttaaaacact tgttcactat ggtatgaact tcttactctt atgcttgtag
     1201 agggccaatc gtggcccgcc acactttggc ccgcctaggc gggccagcaa cactctgagg
     1261 actcgcccag gcgagtgaag ccatgcaaac cattcagctt ttcgagcagc atcgtccgcg
     1321 ccagtggtcg gaagtcgttg ggcagcagaa agcccttgcg gtgctggacc ggctgcggca
     1381 gcgcggaggc ctggcgggcc gcgcgtactt cctgtcgggc gcgaccggca cgggcaagac
     1441 gacgattgcc ctcctcatcg ccgcggaagt cgcggacgat ttttgcgtca cgtcgatcga
     1501 cgacggcgcg caggtcaatg ccgagacgct ggccgaaatg ggccgcgaca aacagcgccg
     1561 cccattcggc cgcggctgct gctggattat caacgaagcg cacaagctgt cgaatgcggc
     1621 gatcacccgg ctgctgggaa tctgcgagca aatgccgccc tggtttaccg tcatcttcac
     1681 cacgacttcg gaagctaaag aagggctgtt tgacgcaaag ctggactcgc acccgctgct
     1741 gtcgcgctgc aacgaattgc cgctggcccg ccgcgacttg gcgaaagcct ttgccgagcg
     1801 cgcccgcatg atagcgcagg tcgaagggct ggacgggcag ccgctggatc gctacatcgc
     1861 gctggccaaa gactgccgca acaacctgcg tgaaatgctc acccgaatcg aaaatggcga
     1921 gatgctcgcc gcgtcgtgac agctcgggcg cagccggccg cggcaatcac cgacgcggct
     1981 ggtccgcccg cgctgttgcg ggtctgtttg ccgttcgttt cacccttctt ggaggctcgc
     2041 taaggcgagc gcagtaccat ggcgcatctt ttctccaccg gtttgttctt tggtcaacgg
     2101 gcctggcacg gcttgggcac gacgctgccg gccgattccc ccgcacggct cagcatcgac
     2161 gattccgttc ggctggccgg cctggactgg caagtcgaga cgcggcccat ctttctggag
     2221 ggaggccagc agataaccgg gcaccaggca gtcattcgca ccgattccac caaggtgctg
     2281 ggagtcgtcg gcgaccgcta ccagccgctg cagaatgcgg accatttccg ctggtttgcg
     2341 ccgttcctgg acacgcgcga agtcgcattt gagacgtgcg gcgcgctgcg ggacgatcag
     2401 ctcgtttggg tgctggccaa aatccagcgg ccagacgcgg tcgtttcggc cagcgataaa
     2461 atcgccaagt acctgctgct ttccagcagc catgacggca gcgcagcaac tagcgtcggc
     2521 tactgcccga tccgcgtcgt ctgctggaat acgcttagca ccggcctgtc gcatcaggcc
     2581 agcaagctat ttaaggtccg ccacacgcgc agccaggcgg agacgctggc cgcgatccgc
     2641 gagacgatca atctggccga cgagacgttt gaggcgaccg cggccaatat cgcaagctgt
     2701 tggcctgctc gatttctccc gccgatttgc ggcgctacgt caagctagtc ctagagctgc
     2761 ccgaggacga ggcggcctat agcggccgcg cccgcaactc gctggctgat gttgtggagc
     2821 tggcccgccg cggcgtgggg aacgacggca agacggtatg gagcgcctac aacggcgtga
     2881 cgcactacgt cacgcatgcc tatggccaca gcgccgattc ccgtctccgc gccaattggt
     2941 atggcgaagg ccggcgaatg aatgaccgcg cattgaccct cgccctgcag gtcgcaggct
     3001 aatagctcgg gcgcagccgg ccgcggcaat caccgacgcg gccggtccgc ccgcgctgtt
     3061 gcgggtctgt ttggatttcg tttccccatt cgttggaggc tcgctgcggc gagtgcagca
     3121 ccatgcaatc tcacgttctg attgaagtcc gcggcggcgt cgccgaatac cagacggctg
     3181 gcccggtggc cgttacgctg attgattacg acaacgatcc tactgccgaa ttcccgcacg
     3241 attgggataa cgttgatccg gctgcgacga gcaggcccgt ttattacctg tcgcagctcg
     3301 atccggcgga gcgcaacgtc ctccgcaata ctctcgcgcc gggacacgat gacttgctgg
     3361 ccgcgtgcga ggcggttctg gagtctctgg agcggaatct cgcagccgac tatctgcccg
     3421 aaacgagggc cgccctcgct gatgccatcg ccaaggcgaa aggcgggggc caatgacgat
     3481 tcgcgagctg ctgcagggcg tcggacgcaa cggactattg gccgcctggc ttgtcgctcc
     3541 tggcctgcga gggcgacgac cagtgggaag tggcgctcga tgtcgaggag ctggagctgg
     3601 ccgaagaggg cgaagacatc gacctggacg acttccatcg cttccgcggc gagctatacc
     3661 gattgctggc tcagctcgac cagccagacc tggcttggcc cagcgcccgc gagatcgagc
     3721 tgcaatcgcc gccggcctgt cgccggcgga atacgcttcc caggtcctgc gcgattccgc
     3781 ttgaagagcg cgcggagaaa ggcgacgatg ccgccatggc caccaaagag ctgtgcgaca
     3841 agtacggcaa gaactcggcg ctgtcgatca acgccgccat ccgggccgag ctgcccggcg
     3901 gcagcgagct gctcgacgcg ctggagatcg cccacaaggc cgccggccgc cgggacgatc
     3961 cggccgcggc caccgtccat cgcatcctcg aatcgctccg cgccgcgaaa gccgcgctgg
     4021 aggccgaaat ccgccggcgg tacgacatgc cgccgcgcga tgagcgcggc gagccccaag
     4081 gctaaagagc cgccagcggc cggctgacta acgagtgcgg cgacgcaacg gccgggagcc
     4141 tcttacggaa aatggcgggt gcggcgaagg cgtcaatccc gcctttttcg tgcgcaattc
     4201 ttgggcagac acctgctctg aggtgtcaac gactgatagt cgattgacgt aagccatttg
     4261 cccacaacca gtttgccgtg ggctgagcaa ggtttcctga atcccatact ggttggaaag
     4321 ccgcccgggc gctagccgac gattcgcagg gcgaatccac cggtggtcga ctcggctgtt
     4381 ggctgtgctt tgccacgcgc gcccgacttg gcttgtcgcg acgctgcttt gcgcggctca
     4441 gcatctgctt ccgcggcttc gcgctcagcc ttctctttcg caacgacttc gggataaagc
     4501 cagcggcgga cgtgatcgga cgcagccctt agccgatcgt ccgcgatccg ctcgcgatag
     4561 gtcgcggcca tgctggcgtc ggcgtggccc atgatgctgt tgacggccgg ctggtcgcgg
     4621 cactcgtcgg ccacagtgcg gaacgtatgc cgcaggtcgt agaacgtcct ggccacgtct
     4681 tgctctttgg ccagcttgcc gaattcgcgg gacactggat tgtggaccgc ggaccctgcc
     4741 caacttgcgc cgtacttcgt cacaaacacc gtggcctcgg cgtcgtcgct cttgggtgtg
     4801 ggccgcttgg ccagcgccgc ctttatcgcc ttgacggtct cgggccacag ggggcatcgg
     4861 tgatcgatgc ccgtcttcgg ccgatggtaa ttcacccatc cgcccgtcag gtcgagcgcc
     4921 gcgagcggca gtgtgccgca gtccgaattg ccgaagccac agttgatgcc cagcaggatc
     4981 atcgcctt
//
LOCUS       DASRJB010000619         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2719484, whole genome shotgun sequence.
ACCESSION   DASRJB010000619 DASRJB010000000
VERSION     DASRJB010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719484"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFB80_16370"
     CDS             complement(<1..912)
                     /locus_tag="VFB80_16370"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35406.1"
                     /translation="MFGASGRGPSYLRRSSCAAFTLVELLVVIAIIGMIVALLLPAVQ
                     SAREAARRTQCQNHLHQIVVGMHHFEDVHKHLPAAYTTTGTIPGVLGVPGWGWGMWVL
                     PFTEQANLYQAAHPDSARFGGGVNPAQPDSVSQARLAIYRCPSDPARDKNPERLLHAT
                     SNYRAVAGQMSLAVFIADFDYGGVLYQNSRTRLAEITDGTSSTLAIGECILDERADKR
                     AALWTGMAGTRPLPPDSALAVWVSDVMWSVDDADSRINGSAPQAFSSRHPGGAFFAYC
                     DGSVRFFSDTADMSLAKWAAGRDDGNVT"
     gene            complement(1043..2005)
                     /locus_tag="VFB80_16375"
     CDS             complement(1043..2005)
                     /locus_tag="VFB80_16375"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HZN35407.1"
                     /translation="MPKPKGAVRILHQVLVGALICLVSIDTAAACRLFGKRRNRRSCC
                     PPPPTCGCESPPPTCGCDAVSPKAAVESAIDSPPASSVGPMLEDAAPMPVPMPDSPLA
                     PIQIPPAPPVPADPPMPPKAVQPAPPKPVERATPKPAEPAKAAEPMPAPATVTPAPVA
                     PAPATRRPAPVTPAAPAPAPRAAPRPALDSDDPFAPAKPATPAAPAPATPEDDDPFAP
                     IKARPAAVVTDPVRQQPRLPAALVLDDQGRLPARLWTDDSGFFQVRGRLILILEGKVR
                     LLKETGRTTTVPLARLSPADRAYIEQVIGHYGSDLSRPSRVAVR"
     gene            2228..3235
                     /locus_tag="VFB80_16380"
     CDS             2228..3235
                     /locus_tag="VFB80_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35408.1"
                     /translation="MIVVTKDLTKIYGELAALSRCSLAVEAGEVFGLLGPNGAGKTTL
                     LRLLLGYLRPTAGVAQVAGFDCVRQSVAVRRNVAYLPAEAAMFPHMRGRDALRFFAEI
                     RPGGELARSLRLAERLELDISRKVSFMSTGMKQKLALAATLSAQTPIYILDEPTANLD
                     PTVRATVLALVAEARAAGKTVMFSSHVLSEVEEVCDRVVILRSGQLVHTQLMSQLRRQ
                     HRIVAELAGPLPAIPESLSDQLALVSASDREAIFETPGELAPLLKWLATLPLADVRIE
                     PIGLRTVYQRFHSENGGPSKPPASGQRELADGAAPEVPLHYREAHASRSPVPRLPISD
                     S"
     gene            3238..>3869
                     /locus_tag="VFB80_16385"
     CDS             3238..>3869
                     /locus_tag="VFB80_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN35409.1"
                     /translation="MNRAVLKKCLLEARLLFAATAALLFAFCWVRVFIVSRLQMSQFA
                     AIIEQIWDQFKDFSPVPLDQLLSYTGRIAIGYNEPIVVFGISIFAIARGSDAVSGELG
                     RGTLEMLLAQPVSRLQVLYTQALVTVACLALLCGLTWAGTTVGIYTTRVKEDRPPPTL
                     KLPWFKAGIPLPVRNPEKMIVPMREKTDPKFFMAGAVNLLCFGVALAGFST"
BASE COUNT          605 a         1257 c         1370 g          637 t
ORIGIN      
        1 ggtcacgttg ccgtcgtccc gcccggccgc ccacttcgcc agcgacatgt ccgcggtgtc
       61 cgagaagaac cgcaccgagc cgtcgcagta tgcgaagaag gcgccgcccg gatgtcggct
      121 ggagaaggcc tggggagcgg agccgttgat gcgggagtcc gcgtcgtcca cggaccacat
      181 cacgtcgctc acccagaccg ccagggccga gtcgggcggc agcggccgcg tcccggccat
      241 tccggtccac agggcggccc gtttgtcggc tcgctcgtcg aggatacatt cgccgatcgc
      301 cagcgtgctc gacgttccgt cggtgatttc cgccaggcgg gtccggctgt tttgatacag
      361 cacgccgcca tagtcgaaat cggcaatgaa aacggcgagc gacatctggc ccgcgacggc
      421 gcggtagttc gacgtcgcgt gcagtagccg ctcgggattt ttgtcccggg cggggtccga
      481 gggacagcgg tagatggcga gcctggcctg gctgacggag tcgggctgag ccgggttgac
      541 tccgcctccg aaccgcgcgg agtcgggatg ggcggcctga tagaggttcg cctgttccgt
      601 gaacggcagc acccacattc cccagcccca gccgggcacg cccaaaacac cgggaatcgt
      661 tcccgtcgtc gtgtaggccg cggggaggtg cttgtgaacg tcctcgaagt ggtgcattcc
      721 gacgacgatt tggtgcaggt gattctggca ctgcgttcgc cgggccgctt cgcgagccga
      781 ctgcaccgcg ggcaacagca gggccacgat cattccgatg atcgcaatga cgaccagcaa
      841 ctccacgagc gtaaacgccg cgcaagagct gcgccgcaaa tacgatggac ctcgcccgct
      901 ggccccgaac atggaagcgc ctcaaccaga acgttccgct gcctcacctc ctgccgctct
      961 tgtctgcccg ccggaaaagc taaccgagcg cgggcgcaga atcaaccaat acgcccgctg
     1021 atgctagcga ccggggcgag agctaccgga cggcaacccg cgacggccga gacaaatcgg
     1081 agccgtagtg gccgatgacc tgctcgatgt acgcgcggtc cgccgggctc agccgggcca
     1141 gcggtaccgt cgtcgtgcga ccggtttcct tgagcaggcg gaccttcccc tcgaggatca
     1201 ggatcagccg gccgcggacc tggaagaagc ccgaatcgtc ggtccacagg cgggcgggca
     1261 gccgcccttg atcatcgagc acgagcgccg cgggcaaacg aggctgctgc cggacgggat
     1321 cggtcaccac ggccgcgggc ctggccttga tcggcgcgaa gggatcgtcg tcctcgggcg
     1381 tcgcgggagc cggtgcggca ggcgtggcgg gcttcgccgg ggcaaaggga tcgtccgaat
     1441 cgagcgcggg ccgcggcgcg gcccgcggcg ccggagccgg cgcggccgga gtcactggag
     1501 cgggccgcct ggtggccggc gctggagcga cgggcgcggg agttactgtc gcaggcgccg
     1561 gcatcggctc agcagccttc gcgggttctg ccggcttggg cgtggctcgt tctaccggct
     1621 tgggcggagc gggctggacg gccttcggcg gcatgggtgg atcggctgga acaggcggag
     1681 caggcgggat ttgaatcggg gccagcggcg agtcgggcat cggcacgggc atcggcgcag
     1741 cgtcttccag catcggaccg acgctgctgg ccggcggcga atcgattgcc gactcaacag
     1801 cggctttcgg gctgacggcg tcacagccgc aagtgggcgg ggggctttcg cagccgcaag
     1861 tcggcggcgg cggacaacag ctgcgtcgat tgcggcgttt tccgaacagc cggcaggccg
     1921 cggcggtgtc gatcgaaacg aggcagatca gcgctccgac gagcacttga tgcaagatcc
     1981 ggacagcgcc ttttggcttg ggcatcacgt tgcgagcctc cgcgcgggga aacgtcacct
     2041 tcctacgcta cggcgcggat tgccgcgggc aattccgccg ccgcccagtg cgcggcaaga
     2101 cgctatggcg gccagggcaa cgatagcggt cgcgccggcc ttgcgggcgg catgggaaat
     2161 gatgggtgat cgtcccgttg ccggtttgcc gcctggtgcg gacaattgac ggttcgagga
     2221 actcgcattg atcgtcgtaa ccaaagacct gaccaagatt tatggcgagc tggccgcttt
     2281 gagccggtgc tcgctcgccg tggaagcagg cgaggtcttc gggctgctcg ggcccaacgg
     2341 cgcgggcaag acgacgctgc tgcggctgct gctgggctac ttacgtccca cggcgggagt
     2401 ggcccaggtc gcgggcttcg attgcgtgcg gcagtcggtg gccgtccggc ggaacgtcgc
     2461 ctatttgccg gctgaggccg cgatgtttcc gcacatgcgc ggccgcgacg ccctgcggtt
     2521 ctttgccgag attcgccccg gcggcgagct ggcgcggtcg ctgcggctgg cggagcggct
     2581 ggagctcgat atcagccgca aggtgagctt catgtccacc ggcatgaagc agaagctcgc
     2641 gctggcggcg acgctctcag cgcagacacc gatctacatc ctcgacgagc cgacggccaa
     2701 cctcgatccc acggtgcggg cgaccgtgct ggcgctggtg gccgaggcgc gggcggcggg
     2761 caagacggtg atgttctcgt cgcacgtgct gtcggaggtg gaggaggtct gcgaccgcgt
     2821 cgtgatcttg cggtcggggc agctcgtgca cactcagctc atgtcgcaac tgcggcgcca
     2881 gcaccggatc gtcgccgagc tggctgggcc gctgccggcg attcccgaat cgctcagcga
     2941 ccagctcgca ctcgtatcgg cgagcgaccg ggaggcaatc ttcgagacgc ccggcgagct
     3001 agccccgctg ctgaagtggc tggccacgct gcccttggcc gacgtgcgga tcgagccgat
     3061 cggcctgcgg acggtgtatc agcggttcca ttccgagaac ggcgggccgt cgaagccacc
     3121 ggcgagcggc cagcgtgagc tggctgatgg cgccgcgccc gaagtgccgc tgcattatcg
     3181 ggaggctcac gcctcccgct cgcctgtccc ccgactcccg atctccgact cctgaccttg
     3241 aaccgcgccg tcctgaaaaa gtgcctgctg gaagcccgcc tgctctttgc ggcgacggcg
     3301 gcgctgctct tcgcgttttg ctgggtccgc gtgttcatcg tcagccggct gcagatgagc
     3361 cagttcgcgg cgatcatcga gcagatctgg gatcagttca aggatttttc gccggtcccg
     3421 ctcgatcagc tgctgtcgta caccggccgc attgcgatcg gctacaacga gccgatcgtc
     3481 gtgttcggca tctcgatctt cgccatcgct cgcggcagcg acgcggtcag cggcgagctg
     3541 ggccgcggga cgctggagat gctgctcgcc cagcccgtca gccggctgca agtgctctac
     3601 acgcaggcgc tggtcacggt cgcttgcctg gcgctgctct gcggactcac ttgggctgga
     3661 acgacggtcg gcatctacac gacgcgcgtg aaggaagacc ggccgccgcc gacgctgaag
     3721 ctgccctggt tcaaggcggg aattcctctg cccgttcgaa atccggaaaa gatgatcgtg
     3781 ccgatgcgcg agaaaacgga tcccaaattt tttatggcgg gcgcagtcaa cctgctctgc
     3841 ttcggcgtgg ccctggctgg attcagcac
//
LOCUS       DASRJB010000620        19055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1868913, whole genome shotgun sequence.
ACCESSION   DASRJB010000620 DASRJB010000000
VERSION     DASRJB010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 19055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19055
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868913"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFB80_16390"
     CDS             <1..963
                     /locus_tag="VFB80_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35410.1"
                     /translation="CEIRLKGEPADRGEAPPRGDLRIPGLPRLASVPASAGGRLELAR
                     WITSPDNPLTARVMVNRVWQHLFGDGLVRTVDDFGTNSRPPVQQELLDHLATRFRDDG
                     WSVKRLIRAIVLSRTYRQSSASQPAGQEQNPDNTLLWRMNRRRLEWEPLRDSLLEVSG
                     QLTSERPAGIQVSGIGGKSPKSQVASLLPVDSPYRTVYLPVLRDKLSEEYALFDFPNP
                     CLLQGQREVTTVAPQALFFMNDKFVAKCVRETAERLLADAGPSDAERAAWAYQRVLRR
                     PPTAEETAGTIALVKSLDRDLSDAYHWSALIQALFASAEFRYVR"
     gene            976..2370
                     /locus_tag="VFB80_16395"
     CDS             976..2370
                     /locus_tag="VFB80_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35411.1"
                     /translation="MSPTIPTRSVSEGFPVRPPRRTLLQSTAAGFGWLAFSALHAQWS
                     RSAEPKAYKSPLAPKQPHFTAKAKRVIFLFMAGGPSHIDTFDWKSAMVGMTGGRSKHL
                     GPVFSFSPAGQSGLMIADVFPHLARHADDLCLLNGMHTRVAGHQQATVTLHTGSETFV
                     RPSLGSWVVYGLGSVAEDLPGFITINPLSDLGGAQNYGSAFLPAMYQGTRLSGGARGV
                     PNIENTHLAPGDQRKQLDFVARANRRLLAEDPHDPALAGLIESYELAYKMQTSVPQTL
                     DLSAEPQSIRDLYGLDDPATQNFATQCLMARRLAEKGVRFIQLTSQGWDQHNNLREAH
                     GRNAASIDRPIAGLIADLKQRGMLHETLIVWGGEFGRTARDDKGDGNGRGHANRGYTM
                     WLCGGGVKGGLRYGATDELGENAVSGRIGTHDLHATLLYLLGLDHESLTFHYAGRDFR
                     LTDTSGEVHKQIVA"
     gene            complement(2380..3531)
                     /locus_tag="VFB80_16400"
     CDS             complement(2380..3531)
                     /locus_tag="VFB80_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35412.1"
                     /translation="MSAAAFDDLQSALASGGADAALAQAADLLRREKKHHELFEVLKM
                     QLRRRLGLPLLASEAADGLPEQQRAKLEDGLIEACREVGSALLKEGQIREGWMYLRPV
                     GDRAEAAKLLAQVEATDENYEDLIDVCLHEGVDIGRGYGLVLEHFGTCNAITQYDSAI
                     IRRPRSEQAPAARRLLAQVHADLIASVKADITRQEGQQPKEQTLRDLLLDREWLFQDN
                     AYHLDTTHLAATVRIARVLSEPADLRLALDLTEYGRRLSQQFQYHGDEPFAETYPANA
                     LYFQALLGQNIQEAALYFQTKAELLDPQHHGYGAIETYIDLLARLGRHAEALAAAVKF
                     DVGSIQPLGNAPPLVELARKSGEYTSVLAHCRQKQDLLGFTAALVQSAK"
     gene            3701..4648
                     /gene="hpnC"
                     /locus_tag="VFB80_16405"
     CDS             3701..4648
                     /gene="hpnC"
                     /locus_tag="VFB80_16405"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZN35413.1"
                     /translation="MDLAAELAAYGPEAQSRAISPVEARAYCRRLASSHYENFTVVSW
                     LFPRRLHQHLANVYAYCRWADDLADETANLGPPPSAGMPQRAFPIARDESLRLLDWWE
                     RQLDAMFAGEARHPVFVALAETVREFDLRRQPLADLLVAFRRDQTQTQYETLGELLTY
                     CEKSASPVGRIVLCLGRALNNENVYKSDLICTGLQLANFWQDVRRDYERGRIYIPQEA
                     CRARGWDEARFAAGRCDDDFRELLWPLIQFAEMQLFLGQSLVKDLPRELKLPVRAFIA
                     GGQAILAAIRRQRYDVWSRRPTVGRLTKLRLLAQAWWSP"
     gene            4645..5493
                     /locus_tag="VFB80_16410"
     CDS             4645..5493
                     /locus_tag="VFB80_16410"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZN35414.1"
                     /translation="MTPELDAAFRECRLIARRSSSTFACLFWPLPADQRRGMEALYAF
                     ARRCDDIADGPEPLDHRRARLARWREQLRAALAGHADEAVLLALADTVGRFVVPPHLL
                     WHIVDGVAMDLDQRGYATYAELRKYCEHVASAVGLACLPIWGCRDDEAILPAADCGVA
                     FQLTNILRDLQEDGRQGRVYLPHDELARFRCAPVDWLGPVGTAQLAELLKFQCQRAAG
                     LFEAGERTEKYLDRDPRRVFRLMWGTYRALLAKIERDPLVVLRRRVRLSRPRKLLLAA
                     RTLTVR"
     gene            complement(5515..6459)
                     /locus_tag="VFB80_16415"
     CDS             complement(5515..6459)
                     /locus_tag="VFB80_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35415.1"
                     /translation="MKAYEHSGIVPVSGALMTLGAGSLAALLLGVVYTFAFYHILYVY
                     LNVLFTFGFGAAVGWTVGFVAREGKIRNVPVVGGLALVAAFVGLYSAWGTTAYALVPS
                     SELKQFWNDVGLFPFLPHNIAALMGKLYSVGSWGMTANAPVHGLPLVALWVIEAGIIG
                     AMAIAMSVGQILELPFCEPCDEWVVARAEPHLFAGQGYEAAWSDVKHGAFESLAQTPR
                     ASGDETHYVRVTVSTCPKCDASNYLSVVACENRVNPKGGRYKVEQKLATNIPLTAAQV
                     EIVDAAAFIAPAIGSEQLVSCQSVGSAAIEEPANRFPD"
     gene            complement(6518..7024)
                     /locus_tag="VFB80_16420"
     CDS             complement(6518..7024)
                     /locus_tag="VFB80_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35416.1"
                     /translation="MDESSKKSAGQRQASSEPWTVALHRRLSRAWWYFAIPIVTFLML
                     GFGCYYWATWPARQVADDFQQSLAWADYARAFSLCSPELQKLGNAEKLEQIAEASGEF
                     YRPWAWDSGAWEGNRVQLNAAGSGASRASTLPAAANRRCGCRSRPGAPVPLMTDFPSR
                     SALIAAEC"
     gene            complement(7064..7522)
                     /gene="rpiB"
                     /locus_tag="VFB80_16425"
     CDS             complement(7064..7522)
                     /gene="rpiB"
                     /locus_tag="VFB80_16425"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZN35417.1"
                     /translation="MRITIGADHRGFNLKQQIAGWLKSQGHEVADVGTTSTESTDYPL
                     FAAQVARKVAQGQADRGILVCATGVGMCITANKVRGVRATVAYDEDVARASRQHNNVN
                     VLCMGERLTPEAAQQIVQTWLTTEFEGGRHSRRLGEIADVEQSECGVSRG"
     gene            complement(7538..8680)
                     /locus_tag="VFB80_16430"
     CDS             complement(7538..8680)
                     /locus_tag="VFB80_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HZN35418.1"
                     /translation="MAPLVLDVTAADDPRDAVHRAVQALVEGKVVSLPTETVYVAAAS
                     GLSERAVGRLLALRDGKIDGPAVLAVRSTEEVLDYVPQLPLVGQRLARRCWPGPVTME
                     LADAQPESAIHRLPKQVKAAVLPRGEIRVRVPAHDLMLRVLRLLAGPVVMIGARRPQD
                     GDSVTAQEVVQRLGSGVDVVLDDGRCKFAQRSSIVRVDDRGFQVLRAGVISEANLKRL
                     ASFMVVLVCTGNTCRSPMAEALLKQRLADKLKIKIGELDDRGIVVMSGGISAAPGGRA
                     AAESIQTMRERNLDLSQHESQPLSDRIVRFADVIITMTRGHREAILEQWPDAEPRVHP
                     ISGGRGDVSDPIGGPIEQYRRCAEQLDAYLEDWVEKLPLAELRGEA"
     gene            8878..9660
                     /locus_tag="VFB80_16435"
     CDS             8878..9660
                     /locus_tag="VFB80_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN35419.1"
                     /translation="MVPFQATPLLGHVAVVTGASGGIGRAIALELARCGAALVVHGNR
                     HAERAEDVASEIRALGGDAAVLLADLGAPQTAGEFATAAWNWRSGVDIWVNCAGADVL
                     TGAAAAWPYEQKLAALWRVDVLATIHLSRLIGGRMVSRTDRPGGVILNIGWDQAEQGM
                     AGDSGEMFAAIKGAVMAFTRSLAQSLAPHVRVNGLAPGWIRTAWGAAASQYWQERAAR
                     QSLRGRWGTAEDVARAAAFLASPAADFITGQIIPVNGGFRYE"
     gene            9748..11196
                     /locus_tag="VFB80_16440"
     CDS             9748..11196
                     /locus_tag="VFB80_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HZN35420.1"
                     /translation="MPHHIHFVTGRLAEHALRPMVASLAERVGFAYTIEVLPITVAAL
                     MTPAWIARHVHIPPAATRVIVPGYCEGDLAPVQALTSAKVERGPRDLRQLDEFFGQSR
                     RTSDYGQYDIEIIAEINHAPRLPLAEIVGQARALRADGADVIDVGCDPGEPWGGVGEC
                     VGALKAEGLRVSIDSLQPAEIEPAVRAGAELVLSVNATNRDAARDWGCEVVAIPDDFA
                     TLGGLGETVELLAASGVPLRIDPVLEPIGCGFAASLGRYLEVRRRFPDAEMLMGIGNL
                     TELTDADSAGINVLLLGFCQETGIRSVLTTQVINWSRTSVRECDLARRLVYHAASHRI
                     PPKHLEPRLVTLRDPKVHEFGAENLARLAAQIKDHNYRIYAEGGQVHLLGAGLHLTDH
                     DPFLLFERLLHPGFGGSSDTHEPATNVDPSHAFYLGYEMAKAAIALALGKEYRQDEAL
                     DWGYLTVSEESHRLRKSAQPAVRNTARREAGT"
     gene            11193..12611
                     /locus_tag="VFB80_16445"
     CDS             11193..12611
                     /locus_tag="VFB80_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZN35421.1"
                     /translation="MSFAAETDLPLAVELRPATDVESALVRLAARPHCLLLDSALRDP
                     RLGRYSFLAAEPFEFLTVPADHTDGLGRLEVKLRQWRTAARPDLPPFQGGAAGLLSYD
                     LAHSLELVPSSRFDEFQIPALAVGLYDTVVAIDHVEHRAWLISQGFPEVEPAARRRRA
                     ERRIKEFQEILEATGAATPWFAARTTATRADLAPQFPVAGPAGLSSNFTAREYCAAVE
                     RAIDYIHAGDVFQVNLSQRLLFPARGDALSLYLRLRNCNPAPFAGWFDLGDVQIASAS
                     PERFLSVRGGRVEARPIKGTRRRTAWAEADLFAGDDLLQSEKDRAENVMIVDLLRNDL
                     SRVCTPDSVRVNQLCGLETYQFVQHLVSAVEGQLAPGKTAIDLVRAAFPGGSITGAPK
                     IRAMEIIAELEPTARGPYCGSLGYFGFDGSADLSILIRTITSAGGWWQFPVGGGIVAQ
                     SLPEKEYEETWHKAEGLLRALV"
     gene            12614..13273
                     /locus_tag="VFB80_16450"
     CDS             12614..13273
                     /locus_tag="VFB80_16450"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZN35422.1"
                     /translation="MLLLIDNYDSFVHNLARYLRRLGCETQVVRNDALTPDQIARLRP
                     AAIVLSPGPCTPNEAGHSLEIVKQLAGTIPMLGVCLGHQTIGSALGARIVRAPAPMHG
                     RTSQVEHGGEGLFADLPSPLTVGRYHSLVVDRQTLPDELVVTAQTADGVVMALAHTRW
                     PVFGVQFHPESILTEGGYTILANFLRLAGIAELAELPRMESELPRASEPSQVPSQPVT
                     F"
     gene            13286..13873
                     /locus_tag="VFB80_16455"
     CDS             13286..13873
                     /locus_tag="VFB80_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HZN35423.1"
                     /translation="MSADEPILEGIVVTLDDAGRANIAPMGPRVDRQVSRLVLRPFRT
                     SQTYQNLKRSGCGVFHVIDDVELLAHAAVESLDPQPALVKIDGFPCPRLADCCRWLAF
                     RVDLLDDASERTTIDCRVIQRGEVRPFFGFNRAKHAVLEAAIHATRIGIVPAETIRAD
                     LSRLAVIVQKTAGDQERRAFAFLQTHIELQLGHQT"
     gene            complement(13864..15501)
                     /locus_tag="VFB80_16460"
     CDS             complement(13864..15501)
                     /locus_tag="VFB80_16460"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZN35424.1"
                     /translation="MNQHASHSRSGFWPDLLAGLRQTEEQARQGGGPAAIERQHSKGR
                     LTARERIERLIDRGSPLLELGLWAGYEMYADWGGAPGAGVVTAIGTIGGRRHMLIAND
                     ATVKAGAFFPITAKKVLRAQRIAFASRLPLVYLVDSAGVFLPLQEDVFPDEDDFGRIF
                     RNNAVISAAGIPQYAAIMGNCVAGGGYLPVLCDTVLMTEGSGLFLAGPALVKSAIGQE
                     ISAEELGGAAMHAQISGTIDYREPNDEACLARLRSLIELQRPESDQPPAPFARRESAE
                     PSRPGSDLYELIDRDPRSSYEVKDVLACVVDAGSFDEYKAEYGQSLVCGTARLGGYSL
                     GIVANQRHRVKSARGEFQFGGVLYVDSAEKAARFVMNCNQEWLPILFLQDVNGFMVGR
                     DSERAGIIKAGAKLVNAVSNSRVPKLTLITGGSYGAGNYALCGKAFDPRFIYAWPTAR
                     YAVMGPAQATGTLLDVMVASLKKQGREPDAAELESLRDQVAADYERQTDVRYAAARLW
                     VDAILDPARTRDVLIQSLAVATRYAGEEPFRVGVYQV"
     gene            15826..16545
                     /locus_tag="VFB80_16465"
     CDS             15826..16545
                     /locus_tag="VFB80_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35425.1"
                     /translation="MKANLVNTSVLSIVGLLMLGGVAAAQTFATPSGGVTESFYGGGY
                     GGGYGYFSSTYEEGVLRGLGYLRRSTGEMNYFNSLSNINNQEAAARYLVNREKATETY
                     FRMQQINRAAREATRPERLTAEQQIALARQQAPNGLNPAQYNRELGRLSWPAALSADM
                     FAPERLLLDRAFAARAPSDAGDGSAFHSQVKQLTLAMDAKLKEHLPLLSTTEYMAAKK
                     FILGLSVESRQPMIFGALASK"
     gene            complement(16777..17406)
                     /locus_tag="VFB80_16470"
     CDS             complement(16777..17406)
                     /locus_tag="VFB80_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN35426.1"
                     /translation="MIEIRHKDSGAVLKTVNGESLAGEYFEGAKLAGADLSKQNLQKA
                     DLQRADLRDADLSGANLKGALLNGALVRGAIFDGANMEKADLTDSFALGAGFHGANLN
                     GAIMRYAKLSEADFSESSLVGADMSMAELGCDFSRANLMKADLRGSILAGAVMDGATL
                     LGANLTDAKLAGADMSGARLEGAIMAQGRKLGVGGFKKKEARPWWQFWG"
     gene            17610..18365
                     /locus_tag="VFB80_16475"
     CDS             17610..18365
                     /locus_tag="VFB80_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZN35427.1"
                     /translation="MDFRVQLETFRGPLDLLLFLVRKHEVDIRDIPIVPITEQYLAHL
                     EVLQQLDVNEVGDFLELASTLVEIKSQLVLPRGGEEVQAWDDPRDELVQRLLEYKKYK
                     DAASMLDERSRDWQQHYGRLASDLPPRAVDPAGQPIREVELWDLVSAMGRILRESQAI
                     QPQAIVYDETPIQVYMQRIHGKLVGERRVAFSDMFQPGMHKSAIVGVFLAVLELVRHH
                     SVVAEQDDLHGEIWIVPGEQFDPTKEIAPADEY"
     gene            18408..18626
                     /locus_tag="VFB80_16480"
     CDS             18408..18626
                     /locus_tag="VFB80_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35428.1"
                     /translation="MNGSEFTFSVDLAPGERLSLPQSIVETIGPGRWQIHISPAGNRP
                     DPTRGHGAFLNGYSPEDEGLYDDVRPAR"
     gene            18604..18933
                     /locus_tag="VFB80_16485"
     CDS             18604..18933
                     /locus_tag="VFB80_16485"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZN35429.1"
                     /translation="MTSAQPGEFWVADIPFTDGSGSKKRPILVLWIDGTDVVAAAVTS
                     AAPRSTTDVTLADWKVGALRAPSTVRLSRLDCLEQSLLLARIGSISANDAKAVKQAWT
                     NYVHPQF"
BASE COUNT         3206 a         6306 c         6232 g         3311 t
ORIGIN      
        1 tgcgaaatcc gcctcaaggg agagccggcc gaccgcggcg aggccccgcc ccgcggcgac
       61 ttgcgaatcc ccggcctgcc acggctggcg agcgttcccg ctagcgccgg cggacggctg
      121 gagctcgccc gctggatcac ctcgcccgac aatccgctga cggcgcgggt catggtcaac
      181 cgtgtctggc agcacctgtt cggcgacggt ctcgtccgca cggtcgatga tttcggcact
      241 aacagccggc cgccggtgca gcaagaattg ctcgaccacc tggcaacgcg gttccgcgat
      301 gacggctggt cggtcaagcg gctgatccgg gcgatcgtgc tcagccgcac ttaccggcag
      361 tctagcgcca gtcagccggc aggccaggag cagaacccgg ataatacgct gctgtggcga
      421 atgaaccgcc gccgactgga gtgggagccg ctgcgcgatt cgctgctgga agtctccggc
      481 caactgacca gcgagcggcc ggcgggcatt caggtctcgg ggatcggcgg caagagcccc
      541 aaatcgcagg tcgcgagcct gctgcccgtc gattcgccct accgcaccgt ctatctgccg
      601 gtcctccgcg acaagctctc agaggagtac gcgctttttg attttcccaa tccctgcctg
      661 ctgcaagggc agcgcgaggt gacgaccgtc gccccgcagg cgctgttctt catgaacgac
      721 aagttcgtgg cgaagtgtgt gcgcgagacg gccgagcggc tgctggccga tgccggcccg
      781 tccgatgccg agcgagccgc ctgggcgtat cagcgcgtcc tccgccgccc gccgaccgcc
      841 gaggaaaccg ccggcacgat cgcgctcgtc aagagcctcg atcgcgactt gagcgatgcc
      901 tatcactggt ccgccctgat tcaggccctg ttcgcgtccg ccgaatttcg ctatgtccgc
      961 taactgctta ctgacatgag tccgaccata ccaacccgaa gcgtaagcga gggatttcct
     1021 gttcgacccc cccgccgtac cctgctgcaa tccacagccg ccggcttcgg ctggctggcc
     1081 ttttctgccc tgcatgccca gtggtcgcgc tccgccgagc cgaaggccta taagagcccg
     1141 cttgcgccga agcagccgca ttttaccgcg aaggccaagc gcgtcatctt cctgttcatg
     1201 gccggcgggc cgagccatat cgacacgttc gactggaaat cagcgatggt cggaatgaca
     1261 ggcgggcgga gcaagcatct ggggccggtc ttttcgttct ctccggcggg gcagagcggc
     1321 ctgatgatcg ccgacgtgtt tccgcacctg gcaaggcacg ccgacgatct ttgcctcttg
     1381 aacggcatgc acacccgcgt tgccggccat cagcaggcga ccgttacgct gcacaccggc
     1441 agcgagacct tcgtccgccc gtcactcggt tcgtgggtcg tctacgggct gggctccgtc
     1501 gctgaagacc tgcccggctt catcacgatc aatccgctca gcgacctcgg gggcgcccaa
     1561 aactacggct cggcgtttct gccggcgatg taccaaggca cgcggctgtc gggcggagcg
     1621 cgcggagtgc ccaacatcga aaacacgcac ctcgcgcccg gcgaccagcg gaagcagctc
     1681 gatttcgtcg cgcgtgccaa tcgccgcctc ctggccgaag acccgcacga tccggcgctg
     1741 gccggcctga tcgagtcgta tgagctggcc tacaagatgc agacctcggt gccccagacg
     1801 ctcgacctgt cggccgagcc tcaatcgatc cgcgacctgt acggcctgga cgatccggcg
     1861 acccagaact ttgccacgca gtgcctgatg gcccgccggc tggcagaaaa aggcgttcgc
     1921 ttcatccagc tcaccagcca gggctgggac cagcacaaca acctccgcga ggcccacggc
     1981 cgcaacgccg cgtcgatcga ccgcccgatc gccgggctga tcgccgatct caagcagcgc
     2041 gggatgctgc acgagacgct gatcgtctgg ggcggcgagt tcggccgcac cgcccgcgac
     2101 gacaagggcg atggcaacgg ccgcggccac gccaaccgcg gctacacgat gtggctgtgc
     2161 ggcggaggcg tgaagggcgg cctccgctac ggcgcgaccg acgagctggg cgaaaacgcg
     2221 gtcagcggcc gcatcggcac gcacgacttg cacgccacgc tgctttacct gctgggcctc
     2281 gatcacgagt ccctcacgtt ccattacgcc ggccgcgact tccgcctcac cgacaccagc
     2341 ggcgaagtcc acaagcagat cgtggcatag tccgacgttt cacttcgccg actgcaccag
     2401 cgccgcggtg aacccaagca ggtcctgctt ctgccggcag tgagccagga ccgacgtgta
     2461 ctcgcccgac ttgcgggcca gctcgaccag cggcggcgcg ttgccgagcg gctgaatgct
     2521 gccgacatcg aacttgacgg cggcggccag ggcctcggcg tgccggccca agcgggcgag
     2581 cagatcgatg taagtctcga tcgccccata gccgtggtgc tgcgggtcga gcagctcggc
     2641 cttggtctga aagtacaggg ccgcttcctg gatgttttgg cccagcaatg cctggaaata
     2701 aagcgcattg gccgggtaag tttcggcaaa gggctcatcg ccgtggtact gaaactgctg
     2761 gctcaaccgg cggccgtatt cggtcaggtc gagcgcgagc cgcagatcgg ccggttcact
     2821 aagcacgcgg gcaatccgca cggtcgcggc cagatgcgtc gtatcgaggt gataggcgtt
     2881 gtcctgaaac agccactcgc ggtcgagcag caagtcgcga agcgtttgct ccttgggctg
     2941 ctgcccttcc tgccgggtga tgtcggcctt cacgctggcg atcagatcgg cgtggacctg
     3001 ggccagcagg cgcctggccg cgggcgcctg ttccgaccgc ggccggcgga tgatcgccga
     3061 atcgtactgc gtgatggcgt tgcaggtgcc gaaatgctcg agcaccaggc cgtagccgcg
     3121 gccaatatcg acgccctcat ggaggcagac gtcgatcagg tcttcgtagt tttcgtcggt
     3181 cgcctcgacc tgggccagca gctttgcggc ttccgcccgg tcgcccaccg gccgcaggta
     3241 catccagcct tcgcgaatct gcccctcctt cagcagtgcc gagccgactt cgcggcaggc
     3301 ctcgatcaaa ccgtcctcca gtttcgcgcg ctgctgctcc gggagtccgt cggctgcttc
     3361 gctggccagc agcggcaggc ccaatcgccg ccgcagctgc atcttcaaga cttcgaacag
     3421 ctcgtgatgc ttcttttcgc gccgcagcaa atccgccgcc tgcgccagcg ccgcatccgc
     3481 cccgccagag gccagggcgg attgaaggtc atcgaatgcc gccgcgctca tgatcttgtc
     3541 cctcgagagt tggcaatctt gcagtctaat ggccaatcgc cagcgggggt aggttcggag
     3601 cgcataatgc ttccttgcgc gcagcgaatg caaagtgcaa actgacaagg gccaagtgca
     3661 actggagttg gtctccgttt gtggatttgg acgagacgaa atggacctgg ccgctgaact
     3721 tgctgcctat ggaccggagg cgcagtcccg cgcgatctcg ccggtagaag cgcgggcgta
     3781 ctgccgccgg ctggcgagct cgcactacga gaatttcacg gtcgtcagtt ggctcttccc
     3841 gcggcggctg caccagcatt tggcgaacgt ctatgcctac tgtcgctggg cggatgacct
     3901 ggcggatgag actgccaatt tggggccgcc accaagcgcg ggaatgccac agagggcatt
     3961 ccctatagcg cgggacgagt cgctgcggct gctggattgg tgggagcgac agctggacgc
     4021 gatgttcgcc ggcgaggccc ggcaccccgt gtttgtggcc ctggccgaaa cggtccgcga
     4081 gttcgacttg cggcggcagc cgctggcgga tttgctcgtg gcctttcgcc gcgaccagac
     4141 gcagacgcag tacgagacgc tgggcgagct gttgacgtat tgtgaaaaat cggccagccc
     4201 ggtggggcgg atcgtgctct gcctgggccg ggcgctgaat aacgaaaacg tgtacaagtc
     4261 ggatctcatc tgcacggggc tgcagctggc gaatttctgg caggatgtcc gccgcgacta
     4321 cgagcgcggg cggatttaca tcccgcaaga agcgtgtcgt gcccgcggct gggacgaagc
     4381 tcgcttcgca gccggccgct gcgacgacga ctttcgcgag ctgctctggc cgctgattca
     4441 attcgccgag atgcagctct tcctgggcca gtcgctggtc aaggatttgc cccgcgagct
     4501 gaagctgccc gtgcgggcgt ttatcgccgg cgggcaagcc atcctcgcgg cgatccgccg
     4561 ccaacggtac gatgtctggt cgcgccgccc gacggtcggt cggctgacga agctgcggct
     4621 cctcgctcaa gcctggtggt ccccatgact cccgagttgg atgccgcgtt ccgcgaatgc
     4681 cggctgatcg cgcggaggtc ttcgtccacc ttcgcctgcc tgttctggcc gctgccggcc
     4741 gaccagcggc ggggcatgga ggcgctgtat gccttcgcgc gccgttgcga cgacatcgcg
     4801 gacgggccgg agccgctgga tcatcggcgg gccaggctgg cccgctggcg cgagcagctg
     4861 cgggcggcgc tggccggcca tgcggacgag gcggtgctgc tggccctggc cgacaccgtc
     4921 ggccggttcg tcgtgccgcc gcatttgctg tggcacatcg tcgatggcgt ggcaatggac
     4981 ctggatcaac gcggctacgc gacgtacgct gagctgcgga agtactgcga gcacgtggcg
     5041 tcggcggtgg ggctggcctg cctgccgatc tggggctgcc gcgacgacga ggcaatcctg
     5101 ccggcagccg attgcggcgt ggcctttcag ctgacgaaca tcctccgcga tttgcaggaa
     5161 gacggccggc agggccgggt ctacttgcct cacgacgaac tggctcgctt ccgttgtgcc
     5221 ccggtcgatt ggctgggacc ggtaggaacg gcgcagctgg cggaactgct caagttccaa
     5281 tgccagcggg ccgcggggct gtttgaggct ggggagcgaa ccgaaaaata cctcgaccgc
     5341 gatccgcggc gcgtgttccg gctgatgtgg ggcacctacc gcgcgctgct ggccaagatc
     5401 gagcgcgatc cgctagtcgt cttgcggcgg cgggtccgtc tgtcgcggcc gcggaagcta
     5461 ctgctcgccg cgcggacgct gaccgtgcgg tgagtcggca tgccggtcgg tcggttagtc
     5521 aggaaatcgg ttggccggct cttcgatcgc cgcggaaccg acggactgac aactgaccaa
     5581 ctgctcgctg ccgatagcgg gcgcgataaa cgcggccgca tcgacaatct cgacctgcgc
     5641 cgccgtgagc ggaatgttcg tcgcgagttt ctgctcgacc ttgtagcggc cgcctttggg
     5701 gttgacccgg ttctcgcagg cgacgaccga gaggtagttg ctggcgtcgc acttgggaca
     5761 ggtgctgacc gtcacgcgga cgtagtgcgt ctcgtcgccg ctggcccgcg gcgtctgagc
     5821 gaggctttcg aatgcgccgt gcttcacatc cgaccaggcc gcctcatacc cttggcccgc
     5881 aaacaggtgc ggctcggcgc gggcgacgac ccattcgtcg cacggttcgc agaacggcaa
     5941 ctcgaggatt tgcccgaccg acatggcgat ggccatcgcg ccgatgatcc cggcctcgat
     6001 gacccacagc gctaccagcg gcagcccgtg caccggagcg ttggccgtca tgccccacga
     6061 gccgacgctg tagagcttgc ccatgagggc ggcgatgttg tgcggcagaa acgggaacaa
     6121 gccgacatcg ttccagaatt gcttcagctc ggacgagggc accagggcgt aggccgtcgt
     6181 gccccaggcc gaatagaggc cgacgaaggc cgcgacgagg gccaggccgc ccaccacggg
     6241 cacgttacgg atcttgccct cgcgagcgac gaagccgacc gtccagccca cggccgcgcc
     6301 gaacccgaac gtgaagagca cattcagata gacatacagg atgtgataga aggcaaacgt
     6361 gtagaccacg cccagcagca gcgcggccag cgacccggcg ccgagcgtca tcagcgctcc
     6421 ggaaaccggc acgatccccg aatgttcgta tgctttcatg cggctggtcc cccaggccgc
     6481 tcgcttccaa ggcggatgcg cagcggccgg agcaaaccta gcattcggcg gcgatcaggg
     6541 ccgaccgcga cggaaaatcc gtcataagcg gcactggcgc ccccggccgg ctacggcagc
     6601 cgcagcggcg atttgccgct gccggaagag tggaagctcg agacgcgcca ctgcccgccg
     6661 cgttcagctg gacgcggttg ccttcccacg cgccgctgtc ccacgcccac gggcgataga
     6721 actcgccgct ggcctcggcg atctgctcca gcttctccgc gttaccgagc ttctgcaact
     6781 ccggcgagca aagcgaaaac gcccgcgcgt agtctgccca cgccagcgac tgctgaaagt
     6841 catcggctac ttgccgcgcc ggccaggtgg cccaatagta gcagccaaag cccagcatca
     6901 ggaatgtgac gatgggaatg gcgaagtacc accaggcccg actcagccgg cgatgaaggg
     6961 cgaccgtcca cggctcgctg gaggcctgcc gctggccagc ggattttttc gacgactcgt
     7021 ccatcccggt aattcgccgg acgtcggcca atatcggtcc ggcctacccg cggctgactc
     7081 cgcactcgga ctgttcaacg tcggcaatct cgccgaggcg tcggctgtgc cgtccgcctt
     7141 cgaattcggt ggtcagccag gtctgcacga tctgctgggc ggcctctggc gtgaggcgtt
     7201 cgcccatgca gagcacgttc acattgttgt gctgccgcga ggcgcgggcc acgtcttcgt
     7261 cgtacgccac ggtggcccgc acgccgcgga ccttgttcgc ggtaatgcac attcccacgc
     7321 ccgtcgcaca cacgaggatg ccgcgatcgg cctggccctg ggccactttt cgggcgactt
     7381 gcgcggcgaa gaggggatag tcggtgctct cggtcgaggt agttcccacg tcggccacct
     7441 cgtggccctg cgacttcagc cagcccgcaa tttgctgctt gagattgaag ccgcgatggt
     7501 cggcgccaat ggtgattcgc atgggtggac tccgcaatta ggcctcgccg cgcagctccg
     7561 ccagcggcag cttctcgacc caatcttcca ggtacgcgtc gagctgttcg gcgcagcggc
     7621 ggtattgctc gatcggtccg ccgatcggat ccgacacatc gccgcgaccg cccgagatcg
     7681 gatgcacccg cggctcggca tccggccatt gctcaagtat cgcctcgcga tgaccgcggg
     7741 tcattgtgat gatcacgtct gcaaaccgca cgatccggtc cgagagcggc tggctctcgt
     7801 gctgcgacag atcgaggttg cgttcgcgca tggtctggat cgactcggct gccgcgcgcc
     7861 cgcccggcgc cgcggagatg ccgccggaca tcaccacgat gccgcggtcg tccaactcgc
     7921 cgatcttgat cttcagcttg tcggccagcc gctgcttgag cagcgcttcg gccatcgggc
     7981 tgcggcacgt gttgccggtg cagaccagca ccaccatgaa gctcgccagc cgcttgagat
     8041 tcgcctcgga gatgacgccc gcccgcagca cttgaaaacc gcgatcatcg acccgcacga
     8101 tgctcgaccg ctgggcgaat ttgcagcggc cgtcatcgag caccacatcg acgccgcttc
     8161 ccaagcgctg gacgacttcc tgggccgtca cggaatcgcc gtcctgcggc cgccgcgcgc
     8221 cgatcatcac gacgggccca gccagcagcc gcaacacgcg cagcatcaag tcgtgtgccg
     8281 gcactcggac acgaatctcg ccgcggggaa gcacggccgc cttcacttgc ttcggcagcc
     8341 ggtggatggc actctccggc tgagcatccg ccaattccat ggtcaccggg ccgggccagc
     8401 agcggcgggc caggcgctgg cccacgagcg gcagttgcgg cacgtaatcg agcacttcct
     8461 ccgtgctgcg gacagccagc acggccgggc catcgatttt cccgtcgcgc agggccagca
     8521 gccgcccgac cgcccgttcc gacaagccgc tggcggcggc gacatagacc gtctccgtgg
     8581 ggagcgagac gactttgcct tcaaccagcg cttgcacggc ccgatggacg gcgtcgcgcg
     8641 gatcatccgc tgcggtgacg tcgagaacta gcggcgccat gcgggatgcc ggaaattacc
     8701 ggctaaaatt cctttgtttc tacacccaaa caggtgttgg aaatgcgaac gccccccaag
     8761 actccatagg caattgtccc gagcgggctg cggcgggtca agtggcgcaa ctccattcgt
     8821 tccttgctgc taggttccga ctgaactaga accgaaccgg aggaaatgat gtcgagcgtg
     8881 gttcctttcc aagccacacc gctgttgggc cacgtggccg tggtcaccgg tgcgtccggt
     8941 ggcatcgggc gagcaatcgc cctggaactg gctcgctgcg gcgcggcgct tgtcgtgcac
     9001 ggaaaccggc acgccgagcg cgccgaggac gttgcgagcg agattcgtgc gctcggcggc
     9061 gacgcggccg ttctcctcgc cgacctgggc gcgccgcaaa cagccggcga atttgcaacg
     9121 gcggcttgga actggcgcag cggcgtggac atctgggtga attgcgctgg ggccgatgtg
     9181 ctcacgggtg cggcggcggc ctggccgtat gagcagaaac tcgcggctct gtggcgggtc
     9241 gatgtgctgg ccacgatcca cctgtcgcgg ctcatcggcg ggcggatggt gtcgcgcacc
     9301 gatcgcccgg gaggcgtgat cctcaacatc ggctgggacc aggccgaaca aggcatggcc
     9361 ggcgacagcg gtgagatgtt cgccgcgatc aagggcgcgg tgatggcgtt cacgcggagc
     9421 ctggcccagt cgctcgcgcc gcacgttcgc gtcaacggtc tcgcaccggg ttggattcgc
     9481 accgcttggg gtgcagcagc gtcgcaatat tggcaggaga gggccgcgcg gcaatcgctc
     9541 cgcggccgct ggggcacggc cgaggacgtg gctcgggccg cggccttttt ggcttcgccc
     9601 gctgcggatt tcatcacggg tcagatcatt cccgtcaatg gcgggttcag gtatgagtag
     9661 gagcagtgag tggtgagtgg tcggaggtga gtgggcgact gcattttcgc ttgccactca
     9721 ccactcacca ctcgccactc agcccacatg ccccaccaca tccacttcgt caccggccgg
     9781 cttgccgagc atgcgctgcg gcccatggtg gcttcgctgg cggagcgggt gggctttgcg
     9841 tacacgatcg aggtgctgcc gatcaccgtc gcggcgctga tgacgccggc ctggatcgca
     9901 cggcacgttc acattccgcc cgcggcgacg cgggtgattg tccctggcta ttgcgaaggg
     9961 gatctagcgc ccgtgcaggc gctaacgagc gctaaggtcg agcgcgggcc gcgcgacctg
    10021 cggcagctcg atgagttttt tggccagtcg cgccgcacca gcgactacgg ccagtacgac
    10081 atcgagatca tcgccgagat caatcatgcg ccgcggctgc cgctggccga gatcgtcggg
    10141 caggcacggg ccctgcgggc tgacggtgcg gacgtgatcg acgtcggctg cgatccgggc
    10201 gagccctggg gcggcgtcgg cgagtgcgtt ggggccttga aagccgaagg cttgcgggtc
    10261 tcgatcgaca gccttcagcc agcggaaatt gaaccggccg tgcgggccgg cgcggaactg
    10321 gtgctgagtg tcaatgccac caatcgggac gcggcacgcg actggggctg tgaggtcgtc
    10381 gcgattcccg acgatttcgc cacgctcggc ggcttgggcg aaaccgtcga gctgctggcg
    10441 gcctccggcg tgccgctgcg gatcgacccg gtgctggaac ccatcggctg cggctttgcg
    10501 gcgagcctcg ggcgctactt agaagtccgc cgccgctttc ccgacgccga gatgctgatg
    10561 ggcatcggca acctcacgga gctaaccgac gccgactccg ccgggatcaa cgtgctcctc
    10621 ttgggctttt gccaggaaac gggcattcgc agcgtgctca cgacgcaggt gattaactgg
    10681 tcacggacga gtgttcgtga gtgcgacctg gcccgccggc tcgtctatca cgcggccagc
    10741 catcgcattc cgcccaaaca tttggagccg cggctggtga ccttgcgcga tccgaaagtg
    10801 cacgagttcg gcgcggagaa tctggcccgg cttgccgcgc aaatcaagga ccacaactac
    10861 cgcatctacg ccgagggcgg acaggtgcac ttactcggcg cggggctgca cctgaccgac
    10921 cacgatccgt ttttgctctt cgagcggctg ttgcatccgg gctttggcgg gtcgagcgac
    10981 acgcacgagc cggcgacgaa cgtcgatccc tcgcacgcct tctatttggg ctacgagatg
    11041 gccaaggccg cgatcgcgct ggccctcggc aaggagtacc gccaagacga ggccctcgac
    11101 tggggctatc tcaccgtgag cgaagaaagc catcgactgc ggaagtcggc gcagcccgcc
    11161 gtcagaaaca ccgcacggcg ggaggcgggg acgtgagctt cgccgcggag accgacttgc
    11221 cgctggccgt cgagctgcgg cccgcaacgg acgtcgagtc ggcactggtg cggctggcgg
    11281 cgcggccaca ctgtctgctg ctggacagcg cgctgcgcga cccgcgcttg gggcggtatt
    11341 cgttcctggc agccgagccg ttcgagtttc tgacggtgcc cgccgatcac acggacggcc
    11401 tgggccggct ggaggtgaag ttacgccaat ggcgaactgc ggcgcggccg gacctgccgc
    11461 cgtttcaggg tggtgcggcg gggctattgt cgtacgacct ggcccacagc ctggagctcg
    11521 tgccgtcgtc gcggttcgac gaattccaga ttccggcgct ggcggtcggc ctgtacgaca
    11581 cggtcgtggc gatcgaccac gtcgaacatc gggcgtggtt gatctcgcag gggtttcccg
    11641 aagttgagcc cgcggcgcgg cggcggcggg cggagcggcg aattaaggag ttccaggaga
    11701 tattggaagc aaccggcgcc gcgacgccgt ggttcgctgc gcgaaccaca gccacccgcg
    11761 ccgacctcgc gccgcagttt cccgttgccg gaccagccgg cctgagcagc aacttcacgg
    11821 cgcgagagta ttgtgccgcc gttgagcggg cgatcgatta catccacgcc ggcgatgtct
    11881 tccaggtcaa cctgtcccag cgattgctgt ttccggcgcg cggcgatgcg ctgtcactct
    11941 acttgcggct gcggaactgc aatcccgcgc ccttcgcggg ctggttcgac ctgggcgacg
    12001 tgcaaatcgc cagcgcgtcg cccgagcggt ttctaagcgt gcgcggcggg cgcgtcgagg
    12061 cgcggcccat caagggcacg cgccgccgca ccgcttgggc cgaggccgac ctgttcgccg
    12121 gcgacgacct gctgcaaagc gaaaaggatc gggccgagaa cgtgatgatc gtcgatctct
    12181 tgcggaacga cctgtcgcgg gtctgcacgc cggacagcgt ccgcgtcaac caattatgcg
    12241 gcctggaaac ttatcagttc gtccagcacc tcgtctccgc agtcgagggg cagctcgctc
    12301 cgggaaagac ggcaatcgac ctggtgcggg cggcctttcc cggcggctcc attacgggag
    12361 caccaaagat ccgcgcgatg gagatcatcg ccgagctgga gccgacggcc cgcggcccgt
    12421 actgcgggtc gctgggttat tttggcttcg acggctccgc ggacttgagc atcctgatcc
    12481 gtacaattac ctcagccggc ggctggtggc agtttccggt cggcggcggc atcgtcgccc
    12541 agtcgctgcc ggagaaggag tacgaagaaa cctggcacaa ggccgagggg ctgctacgag
    12601 cgctggtcta gacatgctgc tactcatcga caactacgac agcttcgtcc acaacctggc
    12661 ccgctacctg cggcggctgg gatgtgaaac gcaagtcgtc cgcaacgacg cactgacgcc
    12721 cgaccagatc gcccgccttc ggcctgcagc gatcgtcctg tcgcccggcc cgtgtacgcc
    12781 gaatgaagcc gggcattcgc tcgagatcgt caagcaattg gccgggacga ttccgatgct
    12841 cggcgtctgt ttgggccatc agacaatcgg ttcggccctc ggcgctcgta tcgtccgcgc
    12901 gcccgcgccc atgcatggcc ggaccagcca ggtcgagcac ggcggcgagg gactgtttgc
    12961 cgacctgccc agcccgctga ccgtcggccg ctatcactcg ctggtcgtcg atcgccagac
    13021 gctgccggac gaactcgtgg tcaccgccca gacagcagac ggcgtcgtga tggcgctggc
    13081 ccacacgcgg tggccggttt tcggcgtgca gtttcatccg gagtcgatcc tgacggaagg
    13141 cggctacacg atcctggcca acttcctgcg gctcgcggga attgctgagc tggccgaact
    13201 gccgcggatg gagagcgaac tgccgagggc gtccgagccc agccaggtac cctcgcagcc
    13261 ggtgactttt tgaggcacga gcgcgatgtc cgccgatgag ccgatcctgg agggaatcgt
    13321 cgtcacgctg gacgatgcgg ggcgggctaa catcgcgccc atgggaccgc gcgtcgatcg
    13381 ccaggtatcg cggctcgtgc tgcggccgtt tcggacttcg cagacttacc agaatctcaa
    13441 acgatcgggc tgcggcgtgt tccacgtgat cgacgacgtc gagttattgg cccatgccgc
    13501 ggtcgaatcg ctcgatccgc agccggcatt ggtgaagatc gacggctttc cctgtccgcg
    13561 gctggcggac tgctgccgct ggctggcgtt tcgcgtggat ttgctcgacg acgcgtccga
    13621 gcggacgacg atcgattgcc gcgtgatcca gcgcggcgaa gtgcggccgt tcttcggctt
    13681 caaccgcgcc aagcacgccg tgctggaagc ggccatccac gccacgcgga tcggcatcgt
    13741 cccggccgag acgattcgcg cggacctatc gcggctggcg gtcatcgtcc aaaagaccgc
    13801 cggtgaccag gagcgccgcg cgtttgcgtt cctgcagacg cacatcgagc tgcaattggg
    13861 gcatcaaacc tgatatacgc cgacgcggaa cggctcttcg ccggcgtacc tcgtcgcgac
    13921 ggccagcgat tgaatgagca cgtcgcgcgt ccgggcagga tcgaggattg catcgaccca
    13981 cagcctggcg gcggcgtagc ggacgtcggt ctgccgctcg tagtcggcgg cgacctggtc
    14041 gcggagcgat tcgagctctg ccgcgtccgg ctcgcggccc tgcttcttca ggctggcaac
    14101 catcacatcg agcagcgtgc ccgtggcttg ggccggcccc atcacggcgt agcgggccgt
    14161 aggccaggca taaatgaatc gcggatcaaa cgctttcccg cacagggcgt agttgcccgc
    14221 cccatacgag ccgccggtga tcagcgtgag cttgggcacg cggctgttgc tgacggcgtt
    14281 gaccagtttc gccccggcct tgatgattcc cgcccgttcg ctgtcgcggc cgaccatgaa
    14341 gccattcacg tcctggagga acaggatcgg cagccactcc tgattgcagt tcatcacgaa
    14401 gcgggcggcc ttctcggccg aatcgacgta cagcacgccg ccgaactgga attcgccgcg
    14461 ggcggacttc acgcggtgcc gctggttggc gacgatgccc agcgaatagc cgccgagccg
    14521 cgcggtgccg cagacgagcg actggccgta ctcggccttg tattcgtcaa agctgccggc
    14581 atcgacgacg caggccagca cgtccttgac ctcatagctc gaccgcggat cgcggtcgat
    14641 cagttcatag agatcgctgc ccggccggct cggctcggcg gactcgcggc gggcaaacgg
    14701 agcgggcggt tgatcgctct ccggccgctg caattcgatc agcgaccgca gccgggccag
    14761 gcaggcctcg tcattgggct cgcggtagtc gatcgtgccg ctgatctggg cgtgcatcgc
    14821 cgcaccgccc agttcttcgg ccgagatttc ctggccgatc gcactcttga ccagcgccgg
    14881 cccggccagg aacagcccgg aaccctcggt catcagcacc gtgtcgcaaa gtacgggcag
    14941 atagccgccg ccggcaacgc agttgcccat gatggccgcg tactgcggaa ttcccgccgc
    15001 cgagataacc gcgttgttcc ggaagatgcg gccaaaatcg tcctcgtcgg gaaacacgtc
    15061 ttcctggagc ggcaggaaca cgccggccga atcgaccaga taaacgagcg gcagtcggct
    15121 agcgaaagca atccgctggg cccgcagcac cttcttggcc gtgatgggaa agaacgcgcc
    15181 ggctttgacc gtggcgtcgt tggcgatgag catgtgccgc cggccgccga tcgtgccgat
    15241 cgccgtgaca actcccgcac ccggcgcacc gccccaatcg gcatacatct cgtagcctgc
    15301 ccagaggccc aattccaaca gcggcgagcc gcgatcgatc aatcgctcga tgcgctcgcg
    15361 tgcggtcagc cggccttttg agtgttgtcg ctcgattgcc gctggtcctc ctccctgccg
    15421 agcctgctcc tcggtctgcc gcaggcctgc taacagatcg ggccagaatc cgctgcgaga
    15481 atgggaagcg tgctgattca tcgatgacgc gcaaagtttg tggcgaaacg gcaacatcct
    15541 cgccgcggcg aacggcatgt cttggatcgc atattctcaa aacgggagaa aaagccgtcc
    15601 cagtctgaga atttgaggcg cctgcgatcg ttctccaaaa acgccccgca cgatttttgt
    15661 cgccgtaagt ccctggccaa ttctactctt tcggcgacgc agttttccgg tggccgctct
    15721 ggcacggccc ttgcttttag ggttcggccg taacagacgt tgaggaagca cgctttgcaa
    15781 gtcggcgagt tgccgcaacc aaacacggaa agtgagaggg tggaaatgaa agcgaacctg
    15841 gtcaatacga gtgtgttgtc gatcgtcggc ctgctgatgc tggggggcgt cgcggccgca
    15901 caaacctttg ccacgcccag cggcggggtg accgagagtt tctacggcgg cggatacggc
    15961 ggcggatacg gctacttctc ttcgacctac gaagagggtg tgctccgcgg cctgggttat
    16021 ctgcggcgtt cgaccggcga gatgaactat ttcaactcgc tgtcgaacat caacaaccag
    16081 gaagccgccg cccgttatct cgtcaatcgt gagaaagcga ccgagaccta cttccggatg
    16141 cagcagatca accgcgcggc ccgcgaagcg acccggcccg agcggttgac cgccgagcag
    16201 cagatcgcgc tggcccggca gcaggctccg aacggtttga atcccgctca gtacaaccgc
    16261 gagctgggcc gcctgagctg gcccgccgcc ctgtcggctg acatgttcgc tcccgagcgg
    16321 ctgttgctgg accgggcctt cgcggctcgt gctccgagtg acgccggcga cggctcggcc
    16381 ttccacagcc aggtgaagca gctgacgctg gcgatggatg cgaagctgaa ggagcacctg
    16441 cccctgctga gcacgaccga atacatggcc gccaagaagt tcatcctcgg cctgagcgtc
    16501 gagtcgcggc agccgatgat cttcggggcc ctggcctcga agtaactgca caacccgcga
    16561 tgagggtggc ccgtggggcg attgatggga tcgtcacacg tccttgtggg atcagaaccg
    16621 gtttgatctc cggccaccct cacccgtaca cccgctcgag ggcgcggcaa gccacggtgg
    16681 tttgtcgcgc ccttcttgct ttcttgagcg atgtccctcg ctcacactct tgcgcgtcgg
    16741 attggtgggg ccggcccttg cacgttggat caggcctcag ccccagaact gccaccaggg
    16801 tcgggcttct ttcttcttga agccgcccac gccgagcttg cggccttgcg ccatgattgc
    16861 gccttccagg cgagcgcccg acatatcggc cccggccagc ttggcatcgg tcagattcgc
    16921 gcccagcaaa gtcgcgccgt ccatgaccgc cccggccaga atagaaccgc gcaggtcggc
    16981 cttcatcagg ttcgcccggc tgaagtcgca gccgagctcc gccatgctca tgtcggcgcc
    17041 caccaggctg gattcggaga aatcggcctc ggagagcttg gcgtagcgca tgatcgcgcc
    17101 gttcaggttc gcgccgtgaa agccggcacc cagggcgaac gaatcggtga ggtcggcctt
    17161 ttccatgttt gcgccgtcga agatcgcccc ccgcacgagc gctccgttga gcagcgcccc
    17221 cttgaggttg gcccccgaca ggtcggcgtc gcggaggtcg gcccgctgca aatcggcctt
    17281 ttgcagattc tgcttcgaga gatctgcccc ggcgagtttg gcgccctcga aatactcgcc
    17341 cgccaacgac tccccattga cggtcttcag gactgcaccg gaatctttgt ggcgaatctc
    17401 aatcatcaac ccacccgggg agaaactgtg ccgcgatcgg ccggccgcgg ccagccgccg
    17461 gtcctgcaaa agatgaatca gcgcctcgtc cgcctgccga aaaatcggca gattatggtt
    17521 tcgtaatata cacaccggcc ggggtcgccg caaatcaact ttgctgtttg ccatggcctg
    17581 ggccgcgcgc caggcgttac gattcccgca tggactttcg cgttcaactg gagaccttcc
    17641 gcgggcccct ggacctgctg ctgttcctgg tccgcaagca cgaggtcgat atccgcgaca
    17701 ttccgatcgt gccgatcacc gagcagtacc tggctcacct ggaagtcctc cagcagctcg
    17761 acgtcaacga ggtcggcgac ttcctggagc tggccagcac gctggtcgaa atcaaatcgc
    17821 agctggtcct gccccgcggc ggcgaggaag ttcaagcctg ggacgatccc cgagacgagc
    17881 tggtccagcg gctgctggaa tacaaaaagt acaaggacgc cgcgagcatg ctcgacgagc
    17941 ggagccgcga ctggcagcag cactatggcc gcctggccag cgacctgccg ccgcgggcgg
    18001 tcgatccggc cggccagccg atccgggaag tcgagctgtg ggacctggtc agcgccatgg
    18061 gccgcattct ccgcgagtcg caggccattc agccgcaggc catcgtctac gacgaaacgc
    18121 cgatccaggt ctacatgcag cggatccacg ggaagctggt gggcgagcgc cgcgtggcgt
    18181 tctccgacat gtttcagccg ggcatgcaca agtcggcgat cgtcggcgtg ttcctggccg
    18241 tcctggagct cgtccgccac cacagcgtcg tcgccgagca ggacgacctc cacggcgaaa
    18301 tctggatcgt ccccggcgag cagttcgacc cgactaagga aatcgcgccg gcggatgagt
    18361 actaatggcc acggtggtat aatagaagca gtggcagagg gttttcgatg aacggctccg
    18421 aattcacttt ctccgtggac ctcgcccccg gcgagcggct cagcctgccg cagtcaatcg
    18481 ttgaaaccat cggtccgggt cgctggcaaa ttcacattag ccctgcgggc aatcggcccg
    18541 atcccacgcg cggccacggc gctttcctga acgggtacag tcctgaggac gaagggttgt
    18601 acgatgacgt ccgcccagcc cggtgaattc tgggtcgcgg acatcccatt tacggatggc
    18661 agcggttcca agaagcgacc gattctggtt ctgtggatcg atggaacgga tgtggtcgca
    18721 gcggccgtca cttcagccgc accgcgttcc acgaccgatg tcacactggc ggactggaag
    18781 gtcggcgcac tccgagcgcc ttcgacggtt cggctttccc gcttggattg cttagagcaa
    18841 tcgctgctgt tggcacgaat tggctcaatc tctgcaaacg atgccaaggc cgtgaagcag
    18901 gcctggacga attacgttca cccgcaattt tgaaactcac caggtctgac gatggacgat
    18961 tcaaagcggc ttgagctgat cggagaggca gttcggtact gccaaagggt caagtcactc
    19021 ggaatgccgc cgtcgtgttg cacaaaggcg ctgcg
//
LOCUS       DASRJB010000621         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2436313, whole genome shotgun sequence.
ACCESSION   DASRJB010000621 DASRJB010000000
VERSION     DASRJB010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436313"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFB80_16490"
     CDS             <1..765
                     /locus_tag="VFB80_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZN35430.1"
                     /translation="GGDELFGGYDRYARIDAIWKRIAWLPAPLRRLASRLYQNVVHER
                     LLGRSGQSFRARALGVTDRRELYAELHRHWERPAEVVIGASGSPLAGDSTRSVPAAFL
                     EQMMALDAAMYLPDCILTKLDRASMAVSLEARVPLLDHRVVEFAWTLPLEFKVRAGTS
                     KWPLRQILGRHLPPSLFERPKRGFGVPIGQWLRGPLREWAESLLAAERLESEGFLRAG
                     PVVAKWREHLAGRANWQYLLWDVLMFQSWLEHRSSA"
     gene            762..1934
                     /locus_tag="VFB80_16495"
     CDS             762..1934
                     /locus_tag="VFB80_16495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35431.1"
                     /translation="MTRPLRILFAIGDMTGGGSQRQLVGILQRLDRGRFVPHLYLVTP
                     GGELLPELPRDVPVYVFGERWEQPAWRFPGQAHWARIRDFSAVLRHEQIDLVYDRTYH
                     MTLTTAGAVRHPVSRVSVIVTDPRLDFETNVERYRWFKRRLLRRAYHTADRVLAVSEG
                     VRTAAIDYYSLPPAKIETHYNAFDIGPIDRRMHEPLPPGEQRTPLRFEITSAGRLHPQ
                     KGFAYLIEAIRILVHERKRQQVHLRILGAGPLEAELRATVARQGLEQHVTLAGFRANP
                     LPYFRQADLFCLSSLYEGMPNALVEAMLCRTPVLATDCKSGPREILDAGRFGRLVPPA
                     DARLLADAIEDALLNPETWRSRIEPARDHIEQTFSPAAAMEPLQNLFESLVPKREA"
     gene            complement(1936..2280)
                     /locus_tag="VFB80_16500"
     CDS             complement(1936..2280)
                     /locus_tag="VFB80_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZN35432.1"
                     /translation="MSAPQPVKQIIAVVKPFLAEKVVEALRRAPLEALTVREVKGYGR
                     QKNYLEEYGQSEYALAFLPKVEIEAWVDDARVSEVVERINSAARTGRMGDGKVFILPV
                     SRFEQAFDLAER"
     gene            complement(2293..2706)
                     /locus_tag="VFB80_16505"
     CDS             complement(2293..2706)
                     /locus_tag="VFB80_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN35433.1"
                     /translation="MPIVKFVKEKKEIDVPAGANLRAEAVKAGINLNQGFNGIGQSIN
                     KVFNCTTLSFGLCGGFCGTCRVLVTKGMENTNKLTLREWVKFKVPVMPPDPIPPLAYV
                     GHEDTMRLACCTTVHGDIEVESGPEVNLFGENFFS"
     gene            3086..4054
                     /gene="epsC"
                     /locus_tag="VFB80_16510"
     CDS             3086..4054
                     /gene="epsC"
                     /locus_tag="VFB80_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZN35434.1"
                     /translation="MASDLRLKEQLPRLTDRIVAGYQEAGSIHYLGHCPLPNYGEIVG
                     CVEDLKELLYPGYRRREGLHIGNVTYHVGDLIDGLHDKLTRQIARALQHEDRVVTKAS
                     TCVNAADYEAKGQSMAITFLERIPDLRQLLATDAQAAYDGDPACKSIDEVVFCYPGYE
                     AITVHRISHELRRLGVPFIPRMMSEWAHKQTGIDIHPGATIGHHFFIDHGTGVVIGET
                     CDIGNHVKIYQGVTLGALSFATDEDGNLVRGHKRHPTLEDGVVIYANATILGGKTVVG
                     HHSVIGSSVWLTHSVEPHTTVTMEKPKLRIRAEVPDELKPELNFSI"
     gene            complement(4051..5403)
                     /gene="accC"
                     /locus_tag="VFB80_16515"
     CDS             complement(4051..5403)
                     /gene="accC"
                     /locus_tag="VFB80_16515"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZN35435.1"
                     /translation="MFKRILIANRGEIALRIIRACKELGIETVCVYSTADRGSQYLEL
                     ADEAICIGPPRGAQSYLKIDQIISAAEVGNVEAIHPGYGFLSENAHFNEVCQSCNIAF
                     IGPTPQAMEALGDKNTARTMAKKAKVPVVPGSDGLITDDDDAVRVAHQIGFPVLIKAT
                     AGGGGKGMRVAGNDLSLKTALLQARTEAQAAFGNSGVYLERYIDRPRHVEVQIIADSR
                     GNVCHLFERECSTQRRHQKLIEESPAPRLPAAQRQAMCDAAVRMVREANYTNAGTVEF
                     IVDQQDNFYFIEVNARIQVEHCVTELITGIDLIKTQIRVAAGEPLPFKQADVVQRGAA
                     IECRINAENPERNFQPCPGLIKQLHVPGGPGVRWDSHAHPGYTVPPYYDSMIGKLLVH
                     RATREEAIACMQRALAELRIEGIQTTAPFHQRILSHTTFAQGLVDTKFVERELLVEQA
                     "
     gene            complement(5557..6036)
                     /gene="accB"
                     /locus_tag="VFB80_16520"
     CDS             complement(5557..6036)
                     /gene="accB"
                     /locus_tag="VFB80_16520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZN35436.1"
                     /translation="MSKPEPISGSVFELDRLRKLVELMKEHDLSEVDLREARQRIRVC
                     RGPKEPPRGAYAAPPPVAAIGAPPAAKAPAAEAANVAVIKSPMVGTFYSRPNPKAEPY
                     VKSGDRVDTTTVVCIIEAMKVFNEIPAEIRGKIVAVLCEDGEAVEFDKPLFKVDTSG"
     gene            complement(6198..>6676)
                     /locus_tag="VFB80_16525"
     CDS             complement(6198..>6676)
                     /locus_tag="VFB80_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN35437.1"
                     /translation="SESKIGDYDFVLIDWGARGRLYHSDLTRVLVTGKLSPQLHSVYG
                     VVLAAQRAAIAAIRPGAVLKEIDGIARKVIEAAGHGERFGHSLGHGIGLAVHELPRLA
                     PDQDGQLAPGMVVTVEPGIYLPEWGGVRIEDDVLVTPDGCEVLTSVPKELDECLVA"
BASE COUNT         1096 a         2204 c         2178 g         1198 t
ORIGIN      
        1 ggcggcgacg agctgttcgg cggctacgac cgctacgcac gaatcgacgc catctggaag
       61 cggatcgcgt ggctgcccgc tccgctcagg aggctcgctt cacggctgta ccaaaacgtc
      121 gtgcacgagc ggctgctggg ccgcagcggg cagagctttc gggcccgcgc gctgggcgtg
      181 accgaccggc gcgagctgta tgccgagctg caccggcact gggagaggcc ggcggaggtg
      241 gtgattggag ctagcggctc gccattggca ggcgacagta cacgtagtgt gcctgctgcc
      301 tttttggagc agatgatggc tctcgacgcg gcgatgtatc tgcccgattg catcctgacg
      361 aagctcgacc gggcgagcat ggcggtcagc ctggaggccc gcgtaccgct gctggaccat
      421 cgcgtggtgg aattcgcctg gacgctgccg ctggagttca aagtccgtgc gggcacgagc
      481 aagtggccgc tgcggcagat cctcggccgg cacttgccgc cgagcctgtt tgagcggccc
      541 aagcggggct tcggcgtacc gatcggccag tggctgcgcg ggccgctccg cgagtgggcc
      601 gagtcgctgc tcgctgccga gcgactggag agcgaaggat ttctgcgggc agggcccgta
      661 gtcgcaaaat ggcgcgagca tctggcgggc cgcgccaact ggcaatacct gctgtgggac
      721 gtactcatgt ttcaatcctg gcttgagcac cggagcagcg cgtgacgcgg cccctgcgga
      781 tcttgtttgc gatcggcgac atgaccggcg gcggcagcca gcggcagctc gtcggcatat
      841 tgcaacggct ggaccgcggg cggttcgtgc cgcacttata cctggtgacg ccgggcggcg
      901 agctcttgcc ggaactgccg cgcgacgtgc ccgtgtatgt ctttggcgag cgctgggagc
      961 agccggcgtg gcgatttccc gggcaggccc actgggcccg gattcgcgat ttctcggccg
     1021 tgctgcgcca cgaacagatc gacctggtct acgaccggac ctaccacatg acgctgacga
     1081 ccgccggcgc ggtccgccat ccggtgtctc gagtatcggt gatcgtgacc gatccgcggc
     1141 tggatttcga aaccaacgtc gagcgctatc gctggttcaa gcggcgcttg ctgcggcggg
     1201 cctatcacac ggccgaccgc gtgttggccg tttccgaggg cgtgcggaca gcggcgatcg
     1261 actactacag cctgccgccg gcgaaaatcg aaacgcacta caacgcgttc gacatcggcc
     1321 ccatcgaccg gcggatgcac gagccgctgc cgccgggcga acaaaggacg cccttacgct
     1381 tcgaaatcac cagcgccgga cggctgcacc cgcagaaggg attcgcgtac ctgatcgagg
     1441 cgattcgcat tctcgttcac gagcgaaaac ggcagcaggt tcacttgcgg attctgggcg
     1501 ccggccccct ggaggcggaa ctgcgggcaa ccgttgcccg gcagggcctg gagcagcacg
     1561 tcacgctcgc cggcttccgc gccaatccgc tgccctactt ccggcaggcc gatctgtttt
     1621 gcctgtcgtc gctctacgaa ggcatgccca acgcgctggt ggaggcgatg ctctgccgca
     1681 cgcccgtgct ggccaccgat tgtaagagcg gcccgcgcga aatcctggac gccggccgtt
     1741 tcgggcggct cgtgccaccg gccgacgcgc ggctgctcgc cgatgcgatc gaggacgcac
     1801 ttctgaatcc agagacgtgg cggtcgcgca tcgagccggc ccgcgaccac attgagcaga
     1861 cgttttcgcc cgcggcggcc atggagccct tgcaaaacct cttcgaaagc ctcgtgccga
     1921 agcgcgaggc gtagcctatc gctccgccag gtcgaacgcc tgctcgaagc ggctgaccgg
     1981 cagaatgaaa accttgccgt cccccatcct ccccgtgcgg gctgcgctgt tgatccgctc
     2041 gacgacttca ctcacgcggg cgtcatcgac ccaagcctcg atctcgacct tgggcaggaa
     2101 ggccagggcg tattccgact ggccgtactc ttccaggtag ttcttctgcc gtccgtagcc
     2161 cttgacctcg cgcacggtga gggcctccag cggcgctcgc cgcagcgcct cgacgacctt
     2221 ctcggccaga aacggcttga cgaccgcgat gatctgcttc accggctgtg gggcagacat
     2281 tcttgtctgc cactacgaaa aaaagttctc gccgaacagg ttcacttccg ggccgctctc
     2341 gacctcgatg tcgccgtgca ccgtcgtgca gcaagccagc cgcatcgtgt cttcgtggcc
     2401 gacgtaggcc aggggaggaa tcgggtcggg tggcatgact ggcaccttga atttcaccca
     2461 ctcccgcagc gtgagcttgt tcgtgttctc catccccttg gtgaccagca cgcggcaggt
     2521 gccgcagaac cccccgcaca ggccgaagct cagcgtcgtg caattgaaga ccttgttgat
     2581 cgactggccg atgccgttga acccctggtt caggttgatc cccgccttga cggcctcggc
     2641 acgcaggttg gccccggcgg gaacgtcgat ttcctttttc tctttgacaa acttgacgat
     2701 cggcatggct gactcgcgtt aagaacgacg gctacctgtt tactttaccg cacgccatgc
     2761 agggcgctaa ccccgctgcc agcgtccagg cacgcacgaa tcggcaggga atttggcttc
     2821 atttcgcgat cccgtactac cgaaaaaaca cggtaagcct tgaatcgggg cgggcttgcg
     2881 cctacgattc ggcgaactgc cgcaagggcg ctccgcctcg cggcaaaagg cacccctcgc
     2941 atgccagctc gcagctatca ccttgggtct tcgcggtgcc agtgtaacgg ctgacgcgct
     3001 gccccgcgcg tcaggactca ggcggtgttc gcagtttgcc cgcttctggc cggcggtcct
     3061 gccggctctt ccggagtaac tcgccatggc ttccgatttg cgtctcaagg agcagcttcc
     3121 gcggctgacc gaccggatcg tcgccggcta ccaagaggcc ggctcgatcc actacctcgg
     3181 ccactgcccg ctgccgaact atggagagat cgtcggctgc gtggaggatc tgaaggagct
     3241 cctttatccc ggctatcgcc gccgcgaagg cctgcacatc ggcaacgtga cctatcacgt
     3301 cggcgacctg atcgacggcc tgcacgacaa gctcacgcgg cagatcgccc gggccctgca
     3361 gcatgaggat cgcgtggtga ccaaggccag cacgtgcgtc aacgcggccg actacgaggc
     3421 caagggccag tcgatggcga tcacgttcct ggagcggatt cccgatctgc gccagttgct
     3481 ggcgaccgac gcccaggccg cctacgacgg cgatccggcc tgcaagagca tcgacgaggt
     3541 cgtgttctgc tatccgggct acgaggcgat cacggtgcac cgcatctcgc acgagctgcg
     3601 gcggctgggc gtgccgttca tcccgcggat gatgagcgaa tgggcccaca aacagaccgg
     3661 catcgacatc catcccggcg cgacgatcgg tcaccacttc ttcatcgatc acggcacggg
     3721 cgtcgtcatc ggcgagacgt gcgacatcgg caaccacgta aagatctacc agggggtgac
     3781 gctcggcgcg ctgagctttg ccaccgacga ggacgggaac ctggtccgcg gccacaagcg
     3841 gcatcccacg ctcgaggacg gcgtggtgat ctatgccaat gccacgatcc tcggcggcaa
     3901 gacggtcgtc gggcaccact cggtcatcgg atccagcgtc tggctcacgc acagcgtcga
     3961 gccccacacg accgtgacga tggaaaagcc caagctccgc atccgcgccg aagtgcccga
     4021 cgagctgaaa cccgaactga acttttccat ttaagcctgc tcaaccagca gctcgcgctc
     4081 gacgaacttg gtatcgacca ggccctgggc gaaggtcgtg tggctgagaa tccgctggtg
     4141 gaagggcgcg gtggtctgga tgccctcgat ccgcagctcg gccagggccc gctgcatgca
     4201 ggcgatggcc tcctcgcggg tcgcgcggtg gaccagcagc ttgccgatca tcgagtcgta
     4261 gtaggggggc accgtgtagc cgggatgggc gtgcgaatcc cagcggacgc cggggccgcc
     4321 gggcacgtgc agctgcttga tcaggcccgg gcagggctga aagttccgct ccgggttctc
     4381 ggcgttgatc cggcactcga tcgccgcgcc ccgctgcacg acgtcggcct gcttgaaggg
     4441 cagcggctcc ccggccgcca cgcggatttg cgtcttgatg aggtcgatcc ccgtaatcag
     4501 ctcggtgacg cagtgctcga cctggatccg ggcgttgacc tcgatgaaat agaagttgtc
     4561 ctgctgatcg acgatgaact cgaccgtgcc cgcgttggta tagttcgctt cgcggaccat
     4621 cctcacggcg gcgtcgcaca tcgcctgccg ctgggcggcc ggcagccgcg gtgcgggcga
     4681 ttcctcgatc agcttctggt gccgccgctg cgtgctgcac tcgcgctcga agagatggca
     4741 gacgttgccg cggctgtcgg cgatgatctg cacctcgacg tgccgcgggc ggtcgatgta
     4801 ccgctccagg tagacgccgg aattgccgaa cgcggcttgg gcctcggtgc gggcctgtag
     4861 cagcgccgtt ttcagcgaga ggtcgttgcc ggcgacgcgc atgcccttgc ccccgccgcc
     4921 ggcggtggcc ttgatcagca cgggaaagcc gatctggtgg gccacacgga ccgcgtcgtc
     4981 gtcgtcggtg atcagtccgt cgctgccggg caccaccggc actttggcct tcttggccat
     5041 cgtgcgagcc gtgtttttgt cgcccagggc ctccatcgcc tgcggcgtgg ggccgatgaa
     5101 cgcgatgttg cagctttggc agacctcgtt gaagtgggcg ttttccgaaa gaaatccgta
     5161 gcccggatgg atcgcctcga cgttgccgac ttcggcggcg ctgatgatct ggtcgatctt
     5221 caggtagctc tgcgccccgc ggggcgggcc gatgcagatc gcttcgtcgg ccagttcgag
     5281 atactggctg ccgcgatcgg ccgtgctgta gacgcagacg gtctcgatcc ccagctcttt
     5341 gcaggcgcgg atgatccgca aggcgatttc gccgcgattg gcgatgagaa tgcgtttgaa
     5401 catggatttc agagtgcaga gggtctggtt cccaagctct gcttgggaac ccgcggcctg
     5461 cgaagctctg cttcgccgct ctcgaagctg agcttcgggg gcgggcgttc ccaagcggag
     5521 cttgggaacg agaggcaggc tgaaagcctg ccccacttaa ccactggtat ccaccttgaa
     5581 caatggcttg tcgaactcga ccgcctcgcc gtcttcgcag agcacggcga cgatcttgcc
     5641 gcggatctcg gctgggatct cgttgaagac cttcatcgcc tcgatgatgc acaccacggt
     5701 cgtcgtatcg acgcggtcgc cggacttcac gtagggctcg gccttgggat tcggccggct
     5761 gtagaacgtg ccgaccatcg gcgacttgat cacggccaca ttcgccgcct cggccgcggg
     5821 cgctttggcg gccggcggag cgccgatcgc ggcaaccggc gggggcgcgg cataagcgcc
     5881 gcgcggcggc tccttcggcc cgcgacagac gcggatccgc tgccgggcct cgcgcagatc
     5941 gacctcgctc aaatcgtgct ccttcatcag ctcgaccagc ttgcgcaggc ggtcgagctc
     6001 aaacacgctg ccggaaatcg gttcaggttt gctcatcgaa agtttccggg acttcggtag
     6061 cggcgacttg gggggcggct tccggcgacg tttatcgggg tcaggcatag attcggtagg
     6121 gatcgccctc tgtggcgatc caaggccgcg gctctttgtc actccaggga acgccacgga
     6181 gggcgttccc tacagcgcta ggccacaagg cactcatcca gttccttcgg tacgctcgtc
     6241 agcacttcac agccgtccgg agtcaccagc acatcgtcct cgatccgcac gccaccccac
     6301 tcgggaaggt agatgccggg ctcgaccgtc acgaccatgc cgggagcgag ctggccgtcc
     6361 tggtcgggcg ctagccgcgg cagctcgtgg accgccaggc cgatgccgtg gcccagcgag
     6421 tgaccgaagc gttcgccgtg gccggcggcc tcgatcacct tgcgggcgat gccgtcgatc
     6481 tccttcagca ccgcgccggg ccggatcgcg gcgatcgccg cccgctgcgc ggcgagcaca
     6541 actccataca cgctatgaag ttggggcgaa agtttaccgg tgaccagcac ccgcgtcaag
     6601 tcgctgtgat agagccggcc gcgagcgccc cagtcgatca acacgaagtc gtagtcgccg
     6661 atcttcgact cggaga
//
LOCUS       DASRJB010000622         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4130219, whole genome shotgun sequence.
ACCESSION   DASRJB010000622 DASRJB010000000
VERSION     DASRJB010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4130219"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFB80_16530"
     CDS             complement(<1..278)
                     /locus_tag="VFB80_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35438.1"
                     /translation="MIASDSNPARPAAGSSEQPIVAEVVTRPRPMQFGLRTLLLLMAV
                     CSVQFAVMSYAGVLPGIALSLLLACAAFAGVFLMGILPGVFAVQQVRQL"
     gene            complement(321..4220)
                     /locus_tag="VFB80_16535"
     CDS             complement(321..4220)
                     /locus_tag="VFB80_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZN35439.1"
                     /translation="MRYRRQAILLVGLLAAARLAAGQETAALPAGVKIVWDAAQAQRE
                     STATRERICLSGLWRWQPAQPAASEVPARGWGYFKVPGSWPGITNYMQKDTQTVIAHP
                     AWQNVAISGVTAAWHQREFSVPREWAGRRIALAAEYVNSLATVYVDGRPVGEISFPAG
                     EVDLTAVCRSGEKHVLSLLVVALPLKSVLLSYTDTAAARQAPAVVARRGLCGDVFLVS
                     TPKAARITDVKVDPSVRRGEIAFETALTGLAADSSYTLLGRVTSPGESPREFRSPPLT
                     TGDLKEGRFTFTEKWRPAKLWDLHTPQNQCELSLTLVDSQGRTLDVSDPVRFGFREFW
                     IGSRDFYLNGSRIFLSAVPLDNAQVSAAAASYAGARESLERLKAIGVNFVYTHNYGCQ
                     PGDHLSFAEILRAADDVGMLVALSQPHFTHYDWQAADADAANGYARHAEFYVRVAQNH
                     PAVVAYSTSHNACGYGEDMNPDMIDGRTDPRSPGEQNNARRALRSEAIIKRFDPSRIV
                     YHHAGGNLGAMHTMNFYPNFVPVQEMSDWFEHWATTGVKPAFTCEYGAPFSWDWGMYR
                     GWYKGERSFGSARVPWEFCLAEWNAQFLGEAAYKIEEPEKANLRWEARQLAAGRLWQR
                     WDYPQNLNLNSKQFTQRDPIVAEYITRNWRAFRTWGVSAISAWEHGQYWDVKSGVERN
                     IRQELPVDWDKLQRPGFSPDFLGQRYERMDMAYERADWTPSAAGQALLRNNQPLLAYI
                     AGKPEAFTSQDHLFVPGETVSKQAIVINNSRQTATCDCTWSLGLPQPLAGSQQVTVAT
                     GEQARIPLELALPPSLAPGDYELRAEFKFAGGDAQAYSLAIHVVPRPRALQVSARIAL
                     FDPPGQTRAWLSKLGLAFSDVDAAADLSGFDVLIIGKSALTLSSAAPSISRVKDGLRV
                     LVFEQTAEVLQQRLGLRVTEYGLREVFPRVADHPILKGISKECLHDWRGSATLVPPRL
                     KYELRPRLGPTVQWCGLTVPRLWRCGNRGNVASVLIEKPARGDFLPIVDGGYSLQYSP
                     LLEYREGAGLMLFCQLDVTGRTESDPIADMLASNLLEYVAASKPVARREAVYAGDAAG
                     KMHLEAAGISAVPFAAGKLPPSAVLIAGPGSGAVLGEDAAALADWLASGGRLVTVGLS
                     EQDARQVLPQLRLRKAEHIAAFFEPPPFRSPLAGVSPADVHNRDPKELPLVMAGATTV
                     GDGVLAWSEDGRIAVCQLAPWQFGGSQQPNLKKTFRRTSFVLSRLLANQGANSETPLL
                     SRFAAPLAAGSKEQRWLSGLYLDQPEEWDDPYRHFRW"
     gene            complement(4230..4748)
                     /locus_tag="VFB80_16540"
     CDS             complement(4230..4748)
                     /locus_tag="VFB80_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35440.1"
                     /translation="MREVLLLVSFLAVGCVALKYPNEWWWVALSSCTLLVFLTALVAV
                     LVDRGSRQAMAIGCAVCMATYGVLVWSSADREFDPYSGRLPTTQIMKPLFQAIVAVTY
                     HDMNTGKLLADYDPASAPTNAPLNVYSLESPDRGQFMLISHLLWALLLAWFGARVACA
                     LYARRIREQQAT"
     gene            4888..>5973
                     /locus_tag="VFB80_16545"
     CDS             4888..>5973
                     /locus_tag="VFB80_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN35441.1"
                     /translation="MKLRSLALSGLFACLAAAAAAAERPNIVLILADDLGYGDLHCFG
                     AQDVQTPNLDRLAAEGLRLTSCYAGHPNCSPSRTALMTGRTPTRVGVRNWIPEDSTMH
                     LRQSEITVARLLKQAGYATCHSGKWHLNGAFNKPTQPQPKDHGFDHWFSTQNNAAPSH
                     KNPSNFVRNGQAAGPQEGYSAHVVVDEALRWLKSGRDAAKPFFLYVCFHEPHEPIAAD
                     EKYARLYPSDDPRFSAHHGNITQMDDAAGRLLRGLDEMKLRENTLVVFTSDNGPEATW
                     PWQGSSGSWRGYYFTHMEGSLRAPFIIRWPNRIPAGRVSNEIVHEVDTYNTFARIAGA
                     QVPKDRPIDGVDQTDFLLGKSDKSNREG"
BASE COUNT         1095 a         2025 c         1896 g          957 t
ORIGIN      
        1 agctggcgaa cttgctgcac ggcgaaaacg cccggcagga tgcccatcag aaagacgccc
       61 gcaaacgcgg cgcacgccaa gagcaggctc agcgcgattc ccggcagcac ccccgcgtac
      121 gacatcaccg cgaactgcac cgagcagacc gccatcaaga gcagcagcgt ccgcaggccg
      181 aactgcatcg gccgcggccg cgtcacgact tcagccacaa tcggctgctc ggagctgccg
      241 gcggcgggcc gcgcgggatt ggagtcggac gcgatcatcg tcactattca actcgcaggc
      301 aaatggatca gtcaaccgcg tcaccagcgg aaatggcgat acggatcgtc ccattcctcg
      361 ggctggtcga gatacaggcc gctcagccag cgctgctcct tgctgccagc cgcgagcggc
      421 gccgcgaagc ggctgagcag cggtgtctcg ctattggcgc cctggttggc cagcagccgg
      481 gagagcacga acgacgtgcg acgaaatgtc ttcttcaggt tgggctgctg gctgccgccg
      541 aattgccagg gcgccaactg acagacggcg atgcggccat cctcggacca ggccaatacg
      601 ccgtcaccga cagtggtcgc tccggccata acgagcggca gctccttggg atcgcggttg
      661 tgaacatcgg ccgggctgac tcccgccagc ggagagcgga acggcggcgg ctcgaagaac
      721 gccgcaatgt gctcggcttt gcggaggcgc agctgcggca aaacttgccg ggcgtcctgc
      781 tcgctgaggc ccacagtcac gagtcgcccg ccggatgcca gccaatcggc cagggcggca
      841 gcgtcctcgc cgagaaccgc gccgctgccc ggaccggcga tcagtaccgc cgatggcggc
      901 agctttcctg ccgcgaaggg aacggcggaa atgccagctg cttccaggtg catcttgccg
      961 gcggcatcgc ccgcataaac cgcttcgcgg cgggcaaccg gcttgctggc agccacatat
     1021 tccagcagat tgctggccag catatcggcg atcggatcgg actcggtccg cccggtgaca
     1081 tcgagctggc aaaacagcat cagcccggct ccctcgcgat attccagcag cgggctgtac
     1141 tgaaggctgt agccgccgtc cacaatcggc aggaaatcgc cgcgggccgg cttctcgatc
     1201 agcaccgagg cgacgttgcc gcggttgccg catcgccaca gccgcggcac cgtgaggccg
     1261 caccactgca cggtcggtcc cagccgcggc cgcagttcgt acttcagccg cggcggcacg
     1321 agcgtcgccg aaccgcgcca gtcatgcagg cactccttcg agatgccttt aagaatcgga
     1381 tgatccgcaa cccgcggaaa cacctcgcgc aggccgtact cggtgacacg caagcccagc
     1441 cgctgctgaa gcacctcagc cgtctgctca aacacgagca cgcggagacc gtccttcacg
     1501 cgcgagatgc tgggcgctgc gctgctgagc gtcagggccg atttgccgat gatcagcaca
     1561 tcgaagccgg acaggtcggc cgcggcgtca acgtcgctaa acgccaggcc gagcttgctg
     1621 agccaggccc gcgtctgacc cggcggatcg aaaagtgcga tcctggcgct cacctgcaac
     1681 gcgcgaggcc gcggcacgac gtgaatcgca agcgaatagg cctgcgcatc gccgcccgcg
     1741 aatttgaatt cggcccgcaa ctcatagtcg cccggcgcga gcgatggtgg cagcgccagc
     1801 tccagcggaa tccgcgcctg ctcgcccgtc gctaccgtga cttgctggct gcccgccagc
     1861 ggctgcggca agccaagcga ccacgtgcag tcgcaggtcg ccgtctgccg cgagttgttg
     1921 atcacaatcg cctgcttgga aaccgtctcg ccgggaacaa acaggtggtc ctggctggtg
     1981 aaggcctcgg gcttgccggc gatatatgcc aaaagcggct gattgttcct cagcaaggcc
     2041 tgtcccgcgg cgctcggcgt ccaatcggct cgctcatagg ccatgtccat ccgctcgtag
     2101 cgctggccca gaaagtccgg actgaagccg ggccgctgca gcttgtccca atcgacaggc
     2161 agttcctggc ggatgttccg ttcgacgccg ctcttcacgt cccagtattg accgtgctcc
     2221 caggccgaga tggccgacac gccccaggtg cggaaagcac gccagttcct cgtgatgtac
     2281 tcggcgacga tcgggtcgcg ctgtgtgaac tgcttggagt tcaaattgag attctgcgga
     2341 tagtcccacc gctgccacag ccggcccgcg gcgagctgcc gggcctccca gcgcagattc
     2401 gccttctccg gctcctcgat cttgtatgcg gcttcgccca ggaactgggc gttccactcg
     2461 gccaggcaga actcccacgg cacgcgggcg ctgccgaacg accgctcgcc cttgtaccag
     2521 ccccgataca tgccccagtc ccacgagaag ggcgcgccat attcgcaggt gaacgccggc
     2581 ttgacgccgg tcgtcgccca gtgctcgaac cagtcggaca tctcctgcac ggggacgaag
     2641 ttcggataga agttcatcgt gtgcatcgcc ccgagattgc cgccggcgtg gtgatagacg
     2701 atgcggctgg gatcgaagcg cttgatgatc gcttcggatc gcagcgcccg ccgcgcgttg
     2761 ttctgctcgc cgggactgcg aggatcggtg cggccgtcga tcatgtccgg attcatgtcc
     2821 tcgccgtagc cgcaggcgtt gtggctggtc gaataggcca cgaccgccgg atgattctgt
     2881 gcgacccgca cgtagaactc cgcgtgccgg gcgtagccat tggccgcgtc ggcatcggcc
     2941 gcttgccagt cgtagtgcgt gaagtgcggc tgcgacaggg cgacgagcat tcccacgtcg
     3001 tcggccgccc gcaggatttc cgcgaaactc aggtgatcgc ccggctggca gccgtagttg
     3061 tgcgtgtaaa cgaaattgac gccgatcgcc ttcagccgct cgaggctctc gcgggccccg
     3121 gcgtagctgg cggcggccgc gctgacctgg gcgttgtcga gcggcaccgc cgacagaaag
     3181 atgcggctgc cgttgaggta gaagtcgcgg ctgccgatcc agaactcgcg gaagccaaag
     3241 cgaacggggt cgctcacatc gagcgtgcga ccctgcgaat caaccagcgt cagtgacagc
     3301 tcgcactggt tctggggcgt gtgcaggtcc cagagctttg ccggccgcca cttctcggtg
     3361 aaagtgaagc gaccctcctt taagtcgccg gttgtgagcg gcgggctgcg gaattcgcgc
     3421 ggcgattcac cgggactggt aacgcggccc agcaacgtgt aactggagtc ggccgcgagg
     3481 ccggtcagcg cggtctcgaa ggcaatctcg ccccgccgca ccgaaggatc gactttcacg
     3541 tcggtgatcc gcgccgcctt cggcgtgctt accagaaaca cgtcgccgca gaggccgcgg
     3601 cgggcgacga ccgccggcgc ttgtcgggcg gccgccgtat cggtgtacga gaggagcacg
     3661 ctcttcagcg gcaacgccac aaccagcagg ctcagcacgt gtttctcgcc cgaacggcag
     3721 acggcggtca gatcgacctc gcccgccgga aacgaaatct cgcccaccgg ccggccatcg
     3781 acatacacgg tggccagcga attgacgtac tcggccgcga gcgcgatgcg gcggccggcc
     3841 cactcgcgcg gaacgctgaa ctctcgctgg tgccaggcgg ccgtaacgcc gctgatagcc
     3901 acattctgcc acgccggatg ggcaatgacc gtctgcgtat ccttctgcat gtagttcgtg
     3961 atgccaggcc agctgccggg gactttgaag tagccccagc ctcgcgccgg cacttcactc
     4021 gcggcgggct gcgccggctg ccatcgccag aggccgctca ggcaaatccg ttcccgcgtc
     4081 gcggtcgatt cgcgctgcgc ttgggccgcg tcccagacga tcttcacgcc tgccggcaag
     4141 gcggctgttt cctgaccggc agccagccgc gcggccgcga gcagcccgac cagtaaaatc
     4201 gcttgccggc ggtatcgcat cggtatccct caggtcgcct gttgttcccg aatccgccgg
     4261 gcgtagagcg cgcaggcgac gcgagcgcca aaccaggcga gcagcagcgc ccacagcaaa
     4321 tggctgatca gcatgaactg gccgcggtcg ggcgactcta gcgagtagac gttcaagggc
     4381 gcattggtcg gcgcacttgc agggtcgtag tccgccagga gcttgcccgt attcatgtcg
     4441 tggtaggtca ccgcaacgat ggcctgaaac agcggcttca tgatctgcgt cgtcggcagc
     4501 cgccccgaat agggatcaaa ttcgcggtcc gcactggacc aaaccaggac tccgtacgtc
     4561 gccatgcaaa cggcacaacc gatcgccatt gcctgccgcg atccgcgatc gacgagcacc
     4621 gcgaccaggg ccgtcaggaa cacgagcaaa gtgcagctcg acagggcgac ccaccaccac
     4681 tcgttgggat acttcagcgc gacgcagccg acggcgagga aactgaccag cagcaagacc
     4741 tcgcgcaacg aaacacgcca cgggctcgct ggcgggcgtg gtggatttgc cggatcggcc
     4801 attacaataa ccccgcagcc cgctgattgt cgccgcaccg acttgggtat tcaaccgcgc
     4861 cgctttcgcc ccatctggaa tccgatcatg aaactccgct cgctggcgtt atccggatta
     4921 ttcgcatgtc tggctgccgc cgccgcggcc gccgagcggc cgaacatcgt gctgatcctg
     4981 gccgatgacc tgggctatgg tgacttgcac tgcttcggtg cgcaggatgt gcagacgccg
     5041 aacctcgatc ggctggcggc ggaaggcctg cgactgacca gttgctacgc gggccatccc
     5101 aactgctcgc cgtcgcggac ggccctcatg accggccgca cgcccacgcg cgtgggtgta
     5161 cgaaactgga ttccggaaga ctcgacaatg cacctccggc aaagcgagat cacggtcgcc
     5221 aggctgctga agcaggccgg ctatgctacc tgccacagcg gcaagtggca tctcaacggc
     5281 gcgtttaaca agcccacgca gccccagccg aaggaccacg gcttcgacca ctggttctcg
     5341 acccagaaca acgccgcgcc gagccacaag aacccgagca atttcgtccg caacggtcag
     5401 gcggccggcc cgcaggaagg ctattcggcg cacgtcgtcg tcgatgaggc tctccgctgg
     5461 ctgaagagcg gccgcgacgc ggccaagccg ttcttcctct atgtctgctt ccacgagccg
     5521 cacgagccga tcgccgcgga cgagaaatac gcgcggctgt acccctcgga cgatccgagg
     5581 ttcagcgccc accacggcaa catcacgcaa atggatgatg ccgccggccg gctactgcgg
     5641 gggttggacg agatgaagct ccgcgagaac acgctcgtgg ttttcaccag cgacaacggc
     5701 ccggaagcga cctggccctg gcaaggctcc tcggggtcgt ggcgcggcta ctacttcacg
     5761 catatggaag gttcgttgcg ggcgccattt atcatccgct ggcctaacag gattcccgcc
     5821 ggccgcgtga gcaacgagat cgtgcacgag gtggatacct acaacacgtt cgccaggatc
     5881 gccggcgcac aggtaccgaa agaccgcccc atcgacggtg tggatcaaac cgatttctta
     5941 ctcggcaagt ctgataagtc caatcgcgag ggt
//
LOCUS       DASRJB010000623         7711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_181167, whole genome shotgun sequence.
ACCESSION   DASRJB010000623 DASRJB010000000
VERSION     DASRJB010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181167"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..92
                     /locus_tag="VFB80_16550"
     tRNA            19..92
                     /locus_tag="VFB80_16550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Met,seq:cat)
     gene            792..983
                     /locus_tag="VFB80_16555"
     CDS             792..983
                     /locus_tag="VFB80_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35442.1"
                     /translation="METRLQLWMQDGALAIAFEPKLTPDQYAELLEVSKESTTREELR
                     ASVMALAKTWGMEAQCDDA"
     gene            1217..1669
                     /locus_tag="VFB80_16560"
     CDS             1217..1669
                     /locus_tag="VFB80_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN35443.1"
                     /translation="MAGRTIAVVGDVYRFLAVGEDTNGKYALFEAVVPPGGGPPPHVH
                     SREEEGFYVLEGEITFQIGGERLVAGAGTFANVSVGTPHSFKNESDRPAKMLISVAPA
                     GLERMFLEVGQPVDEGATTTAPTTKEEIEKLLSVAPRYGIEIRLPRQG"
     gene            2043..2447
                     /locus_tag="VFB80_16565"
     CDS             2043..2447
                     /locus_tag="VFB80_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35444.1"
                     /translation="MRFRLRTLLIAVSILAFASAAAGWSWRRNQAEQQAVKVLAGHRI
                     GLGYTRGLPLGPLEKWFDARLGREFRSDVSSVHLHSFEADEPKVVAALRRLPGLKRVE
                     VDAWPGSQATVAKLQSDFRDTPVEVKVEHVVW"
     gene            complement(2468..4603)
                     /locus_tag="VFB80_16570"
     CDS             complement(2468..4603)
                     /locus_tag="VFB80_16570"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35445.1"
                     /translation="MLSFRYRGGRLCDGVTRREWLRVGGLGVVGLSLTELLARRSAAE
                     EPQQVNRLAAGLSGAMFGRAKNVIFLWLQGGPPQHETFDPKPDAPLEIRGPFKPIQTN
                     VPGMQFCELLPRTAQRADKLAVIRSLSTDDNNHDVSGYWVLTGYPYGPGSARQIKPTD
                     WPYLGSVIKMLKPSERLPALTSVWLPDRMRLNDNVTPAGQTGGFLGSFWDPERFIGDP
                     ADDAYRIDGLSLPVDLPPERMQDRAALLKRLNDYLPLAERASAASGWSELSKFALDLV
                     TSGQAREAFDLSRESAKLRDRYGRHTWGQSCLLARRLIEAGVRLVHVNWPREPGDSAV
                     DNPMWDTHAQNADRLQDVLCPLFDVTFAALLDDLADRGLLAETLVVAIGEFGRTPKIN
                     SLAGRDHWGAVFSCALAGAGIAGGQVFGASDRNGAYPASDPIRPHDLTATIYHLLGIP
                     HHGAFRDRANRPQLLTKGEPLHRLLGDRPATAERCAATGDESLVPPYDLKPLVDVDFR
                     AGKLIAATPPSRDKGWRANSLLAADAGGFGARAEGSPQPHIRLGILEIPGEKGVSLPA
                     GSKVMLAQEIRSARGGQFKFTAAISGGGSSRESFERWFGKALTCRLTLFRFANANKNP
                     LEAVELASESFQPLLVDFDKPAEFAISRFLGSTVPGTNFAIGNGLGVAIVLQSTQSQE
                     MRPGESAYLCIHQVQLEFQARARDESVTV"
     gene            complement(4673..5734)
                     /locus_tag="VFB80_16575"
     CDS             complement(4673..5734)
                     /locus_tag="VFB80_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35446.1"
                     /translation="MRFCSFARWLRPAGGWLSPALVALAALTFVARAPAEGPPDAALQ
                     APAKACPTFRVREQDQIWLVSTRCLGCPSDGPEMPAWQLWQYENATWQPRTTAEFYAA
                     DSADLVTPFYIHGNRIDPGVASSDGLAVYFQLAGKFDDEPPVRFVIWSWPSSQIHGPL
                     RDVRSKAARSDVDAYYLGRFLAGMNPDVRTGLIGYSYGARIACGALHLLGGGTQIGLA
                     IEPGPRPHVRVAMWAAGEHDHWLLPGHYHGQALHEAERWFITYNCCDPVLMRYRFVDP
                     CEHPAALGYSGFAGRYLLPPDLDARIEEVNVTNIVGGTHDMRAYLYSLYIQNRTRDYA
                     LWHELPPLPIAAPALTAAR"
     gene            6009..6230
                     /locus_tag="VFB80_16580"
     CDS             6009..6230
                     /locus_tag="VFB80_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35447.1"
                     /translation="MRKVIGLLILTVALASLSGCHTCRRMTGGWFDRGDRCEPPPPAD
                     CPPGMPRASVLYPGSATVLPGPIEVAPQP"
     gene            6313..7272
                     /locus_tag="VFB80_16585"
     CDS             6313..7272
                     /locus_tag="VFB80_16585"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZN35448.1"
                     /translation="MVRLLVIVAALSIFARPAAGQFGNAPAPPAAGAGGKLESRTQKW
                     EFGVSIRAMGGPCAGLVGTFPLPADWPEQQVKVVGEAISPHVRSHSIRASEGLKQMLF
                     EVPQLAVGETATCFITLEVVKTAQQPPADTSGLVVPKDPPREIRKYLGVSPLIESTHA
                     KVKMLARDLTAGKTSAWEQVQAIQSGVRDRVQFEKETKNVFRGAAGALRDGKADKEDL
                     TATFVAVCRAAKIPARMVWVLDYCYAEFYLDDAAGKGGWYPCIVHDDVPLGAVKDFRP
                     ILEKGDNFKVPEEKAPLRFVPEFLTGKGGGGRPSVEFRRRSAD"
     gene            7316..>7711
                     /locus_tag="VFB80_16590"
     CDS             7316..>7711
                     /locus_tag="VFB80_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35449.1"
                     /translation="MRHLLLIPVICTLAGPARAEEPRGIRLGEATTSQWRFGVIVRAP
                     AGASGIRATLPVPMDWPEQQVKVVRDEKSAGVGGVTYATLDGGVKQMQVNIPRLAAGE
                     EASAIVTLEVTKHRILAPDETTAYQIATKP"
BASE COUNT         1387 a         2470 c         2599 g         1255 t
ORIGIN      
        1 cccgtccgcc gaccccttgg cgggatagct cagttggtta gagcaacggc ttcataagcc
       61 gtgtgtcgcc ggttcaagtc cggctcccgc tactgagtag aaaaccagac gccgaaacgg
      121 gatacgttac ctgccagcgt aaggtagtgc ggcattttcc aattcgccaa accggtagac
      181 taccggcgcg ccgtcgcgga agtcctcgtc caaggagtcg aggtggatgc cgcgggcggc
      241 gcttgcggcg ataatctcag cgaaggtcat gcaaaaaagc ccgctgcggc agggtgtctg
      301 gagtcggacc gacctgcttg gacgctgtag cggcgtacct attgcgtcgg tttggacgtt
      361 tcgtgggcgc gtggccaatt caggaccgcc agcagttggc gaagttgggc gacacctgcc
      421 ccgtggtgct gaagcaggcg tgattacttc tcgtcgtccg tgagcagttg tacaagacga
      481 tcattctctg cctgcgatat gtcgaaggat tcaccgttga tcagttcaat gcgaaacttc
      541 ccatacgcgc catagatacg agcaatgtga acggcgttaa caacggtggg aacttggcca
      601 gtgactttga tccatttcat tgggagctgc tccagtcttc gagtttatag aaggttggcg
      661 atttgctgac gtgtcaacat cccgattttg ccccgtccgc ttcggctggc tggctttctt
      721 atgcgctgct cttgcgcgtg ggtgcgttat cgtactgcac cgcgccgcct gccagagtta
      781 ctctgcccgg catggaaacg cggcttcaac tctggatgca agacggggcg ctggcgatcg
      841 ccttcgagcc taagctaacg cccgaccaat acgccgaact gctggaagtg tctaaggaat
      901 ctacgacccg cgaggaactg cgggcatccg tcatggcgct ggcgaaaaca tgggggatgg
      961 aagcccagtg cgacgacgcg tagcggctcg cctgccgctc acgctggcgg ggccccaact
     1021 acgggcgact ggaagtggaa gccggcgaag gcgggggagt aggattacct tcaacccacc
     1081 acggcgggca agtaggcttt tccagactcg tagctcaccg gtgccccgcc ggctgctgtg
     1141 cggtgtcgct cggcgagcgg cacaacagga ggtgaagctg atggcactga cgcacaggtc
     1201 aacgctcaca aagcctatgg cgggccgcac catcgccgtt gtcggcgatg tgtaccgctt
     1261 ccttgccgtg ggcgaggaca ccaacggcaa gtacgccttg ttcgaggcgg tcgtgcctcc
     1321 gggcggcggg cctcccccgc acgtccacag ccgtgaagag gaaggcttct atgtgctcga
     1381 aggtgaaatc acattccaga tcggcggcga gcggcttgtg gccggggccg ggacgttcgc
     1441 caacgtgtca gtcgggacgc cacactcgtt caagaacgag agcgaccgac cagccaagat
     1501 gctcatatcc gtcgcccccg ccggactgga gcggatgttc ctagaagtag gtcaaccggt
     1561 cgatgaaggc gcgacgacca ccgcgccgac gacgaaggag gagatcgaga agctgctgtc
     1621 ggtcgcaccc cgctacggca tcgagattcg gttgccgcgc cagggctgaa gcacctgacc
     1681 acggcggcgt tacgcgcggc ggggcatgag ccgaagctgc gggagtgccc taagcgccga
     1741 cttatcctga aagccaaatc ggtatcgacc gttttgcgcc ggcccactac cgcgaatccc
     1801 tagcaaaacg tgcacctgct tgccaatgat tgcaaatgca gtgccccaag tgccgcggac
     1861 gggacgtaaa aatgttgagc gaaagtgagc agccccgttt gccaaccgac gatcgcacga
     1921 ggacttaggc gtctataaat gtgctgcctg tgggacggtg tttgccctgg cgcggtaact
     1981 cgcctgtaac aatgcgctgg ccgcgtgccg gggagtgcgc ctaacatcaa ggcatgccgg
     2041 ccatgcgctt ccgcctccgc acgctgctga ttgcagtttc gatcctcgcc ttcgcgagcg
     2101 ccgctgccgg ctggtcgtgg cggaggaacc aggccgagca gcaggcggtt aaagtcctgg
     2161 ccggacatcg gataggcttg ggctacaccc gcggcttacc tctgggcccc ctggaaaagt
     2221 ggttcgacgc ccggctgggg cgggaattcc gctcggacgt cagctcggtc cacctgcaca
     2281 gcttcgaggc ggacgagccc aaagtcgtgg cggcgctgcg gcggctgccc gggctgaaac
     2341 gcgtggaggt agacgcctgg cccggctcgc aggccaccgt ggcgaagcta cagagcgact
     2401 tccgggatac tcccgtggaa gtcaaggtgg aacacgtcgt ctggtaggcc ggctgctcgc
     2461 ggggccgcta aaccgtgacg gactcgtcgc gggcccgggc ctgaaattcc aactggacct
     2521 ggtgaatgca caaatacgcg gattcgccgg gccgcatttc ctgagattgg gtggattgca
     2581 gcacgatggc cacgcccaag ccattgccga tcgcaaaatt cgttccggga acggtcgagc
     2641 ccaggaaccg ggaaatcgcg aactccgcgg gcttgtcgaa gtccaccagc agcggctgaa
     2701 acgactcgct ggccaattcc accgcttcca gcggattttt gttcgcattg gcaaagcgaa
     2761 acagcgtcag ccggcacgtc aatgccttgc cgaaccagcg ctcaaacgat tcgcggctgg
     2821 agcccccgcc gctgatcgcc gccgtaaact tgaattggcc gccccgcgcg ctgcggatct
     2881 cctgcgcgag catgactttc gacccagccg gcagcgaaac acctttctca ccgggaattt
     2941 ccaggatgcc gagccgaatg tgcggctgcg gcgaaccctc cgcgcgagcg ccaaagccgc
     3001 cggcatccgc ggccagcagc gaattggccc gccagccctt gtcgcgcgac ggcggcgtgg
     3061 cggcgatcag cttgcccgcg cggaaatcga catcgaccag cggcttcaag tcgtacggcg
     3121 gcacgaggct ctcatcgccg gtcgcggcac accgctcggc cgtcgccggg cggtcaccga
     3181 gcaagcgatg cagcggctcg cccttggtca agagctgcgg gcgattcgcg cggtcgcgga
     3241 atgcgccgtg gtgcgggatg ccgagcagat gatagatggt tgccgtgaga tcgtgcgggc
     3301 ggatcggatc ggatgccgga tacgcgccgt tgcggtcgct ggcgccaaag acctgcccgc
     3361 ccgcgatgcc tgcgccggcc agcgcgcagc tgaaaacggc gccccagtgg tcgcggccag
     3421 ctagcgagtt gatcttgggc gtgcggccga attcgccgat cgcgacgacg agcgtctcgg
     3481 ccaacagccc gcggtcggcc agatcgtcga gcagggccgc gaaggtcacg tcgaacagcg
     3541 ggcagagcac gtcctgcagg cggtcggcgt tctgggcgtg cgtatcccac atcgggttat
     3601 cgacggccga gtcgcccggc tcgcgcggcc aattgacgtg caccagccgc acgccggcct
     3661 cgatcagtcg gcgggccagc aggcagctct gcccccaggt atgccgtccg tagcggtcgc
     3721 ggagcttcgc cgattcgcgc gacaggtcga acgcctcgcg ggcctgcccg gaagtcacca
     3781 ggtccagcgc gaacttcgag agctcgctcc agccactggc cgccgacgcc cgctcggcca
     3841 gcggcaggta atcgttgagc cgcttcagca gcgcggcccg gtcctgcatt cgctcgggcg
     3901 gcagatcgac cggcaggctc aggccgtcga tccgataagc gtcgtccgcc ggatcgccga
     3961 tgaaccgctc gggatcccag aagctcccca gaaatccgcc cgtctgtccg gccggcgtga
     4021 cgttgtcgtt cagccgcatc cggtccggca gccagacgct ggtgagcgcc ggcagccgtt
     4081 cgctcggctt gagcatcttg atgaccgagc ccagataggg ccaatcggtc ggcttgatct
     4141 gccgcgcgct gccggggccg tacggatagc cggtcagcac ccagtagccg ctgacgtcgt
     4201 gattgttgtc gtcggtggac agcgagcgga tcactgccag cttgtcggcc cgctgagccg
     4261 ttcgcggcaa gagctcgcag aactgcatgc cggggacgtt tgtctgaatc ggtttgaaag
     4321 ggccgcggat ttcgagcggc gcgtcgggct tgggatcgaa cgtctcatgc tgcggcggcc
     4381 cgccctgcag ccacaggaag atgacgttct tcgcccggcc gaacatcgcc cccgacaaac
     4441 cagccgccag ccggttcact tgctgcggct cttcggccgc tgaccgccgc gcgagcaact
     4501 ccgtcaacga cagccccacc actccgagtc cacccacgcg cagccattcc ctccgcgtca
     4561 ccccatcgca caaccgcccg ccacggtaac ggaaggagag catgccattt ctccaagcgc
     4621 ggcccgcata gcgcgggcct cgacagagga tcatagcagc tagacgcacg aattaccgcg
     4681 cggcggtgag ggctggcgcg gcaatgggca gcggcggcag ctcgtgccag agggcgtagt
     4741 cgcgggtgcg gttctgaatg tagagcgaat agagataagc ccgcatgtcg tgcgtgccgc
     4801 cgacgatgtt ggtgacgttg acttcttcga tgcgggcatc gaggtcgggc ggcagcaggt
     4861 agcggccggc gaagccggag tagccgaggg cggcggggtg ctcgcaggga tcgacgaagc
     4921 ggtagcgcat gagcaccgga tcgcagcagt tgtaagtgat gaaccagcgc tcggcctcgt
     4981 gcagggcctg gccgtgatag tggccgggca agagccagtg gtcgtgctcg ccggcggccc
     5041 acatcgcgac ccgcacgtgc ggccgcggac caggctcgat cgccaggcca atctgcgtgc
     5101 cgccccccag caggtgcagc gcgccgcagg cgatccgggc cccgtagctg tagccgatca
     5161 gtcccgtgcg gacgtccggg ttcatccccg ccaggaaccg gcccaggtaa taggcatcga
     5221 cgtcggaccg tgcggccttc gaccgcacgt cgcgcagcgg gccgtgaatc tgcgagctgg
     5281 gccacgacca gatgacgaac cgcaccggcg gctcgtcgtc gaacttgccc gccaactgga
     5341 aatagaccgc caggccgtcg ctgctcgcca cgccgggatc gatgcgattg ccgtggatat
     5401 agaacggcgt cacgaggtcg gccgaatccg cggcatagaa ctcggccgtg gtccgcggct
     5461 gccacgtggc gttttcatac tgccagagct gccaggcggg catttccggg ccgtcgctgg
     5521 gacagcccag gcagcgcgtg ctgacgagcc agatctggtc ctgctcgcgg acgcgaaaag
     5581 tcgggcaggc tttcgccggg gcctgaaggg cggcgtcggg cggaccctcg gccggcgcgc
     5641 gggcgacgaa cgtcagcgcg gccagcgcga cgagtgcggg agagagccaa ccgccggctg
     5701 ggcgaagcca gcgggcaaac gagcagaagc gcatggtgcc gaatcacgtt gcagagctgc
     5761 gagatggaga gcagcctcca tcattgttgc ggcccgccgc aaaaagcaag ctccttcacg
     5821 atttcgtcaa gttcgcggcc gaccggcggg tccggaattc acgggcggcg tccgcacaga
     5881 gcgcacgcac ggcacgccgc gctcgctccg tgcggcgaaa agaccaccgt tgcgtttgca
     5941 ccacagcagc cgcgggctag agttgcccag ccaccacggc aaattcctcg cttcggaggg
     6001 agcaagagat gcgaaaagtg attgggttat tgattttgac ggtcgcgctg gccagcctga
     6061 gcggctgcca cacttgccgg cggatgacgg gcggctggtt tgaccggggg gaccgctgcg
     6121 aacccccgcc acccgccgac tgcccgccgg gaatgccgcg ggccagcgtg ctgtatcccg
     6181 gcagtgcgac ggtcctgccc ggcccgatcg aggtcgcgcc gcagccgtag cgggctttag
     6241 cgactctgca acctatgctt cccagggcgc cgcaaatcgc ggcgtccatt gcgctccagg
     6301 cgtgagggaa gcatggtccg gctgctggtg atcgttgccg cgctctccat ttttgcgcgg
     6361 cccgccgctg gtcaatttgg caatgcgccc gcgccgcccg ctgccggagc gggcggcaag
     6421 ctggagtcgc ggacgcagaa gtgggagttc ggcgtctcga tccgcgcgat gggcggcccc
     6481 tgcgcggggc tcgtcgggac gtttccgctc ccggccgact ggcccgagca gcaagtgaaa
     6541 gtcgtcggcg aggcgatctc gccgcacgtc cgcagccaca gcattcgcgc gagcgaaggg
     6601 ctcaagcaga tgctgttcga agtgccgcag ctcgcggtgg gtgaaacggc aacctgcttc
     6661 atcacgctgg aagtcgtcaa gaccgcccag cagccgccgg ccgatacgag cggcctggtc
     6721 gtgcccaagg atccgccgcg cgaaatccgc aagtatctgg gcgtcagccc gctgatcgaa
     6781 agcacgcacg ccaaggtcaa aatgctggcc cgcgacttga cggcgggcaa gaccagcgcc
     6841 tgggagcagg tgcaggccat tcaaagtggt gtccgcgacc gggtgcagtt cgagaaggag
     6901 accaaaaacg tcttcagagg ggcggcgggc gcactgcgcg atggcaaggc cgacaaggaa
     6961 gacctcacgg caacatttgt ggcggtgtgc cgggccgcaa aaattccggc ccgcatggtc
     7021 tgggttctgg actactgcta tgccgagttc tacctcgacg acgcggctgg caagggcggc
     7081 tggtatccgt gcatcgtgca cgacgacgtg ccgctcggag cggtgaagga ctttcgcccg
     7141 atcctggaaa agggtgacaa cttcaaggtc cccgaagaga aggccccgct gcggttcgtg
     7201 cccgagtttc tgacgggaaa agggggcggc ggccggccct cggtcgaatt ccgccgccgc
     7261 tcggcggatt gacgaccgcg ttgacgcggc ccgcgctagg aagaagacta gcgatatgcg
     7321 gcacttgctc ctgattccgg tcatttgcac cttggccgga cctgctcgcg ccgaggagcc
     7381 gcgcggcatc cgcctgggtg aagccacgac cagccagtgg cggttcggcg ttattgtccg
     7441 cgcccccgcc ggcgccagcg gcatccgggc gacgttgccc gtaccgatgg attggccgga
     7501 gcagcaggtc aaggttgtcc gcgacgagaa gtcggccgga gtcggcggcg tcacgtacgc
     7561 cacgctcgac ggcggcgtga agcaaatgca ggtgaatatt ccgcggctgg cggccggcga
     7621 agaggcctcg gccatcgtca cgctggaagt cacgaagcat cgcatcctcg ctccggacga
     7681 gacaaccgcg tatcaaattg cgacgaagcc g
//
LOCUS       DASRJB010000624         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3284777, whole genome shotgun sequence.
ACCESSION   DASRJB010000624 DASRJB010000000
VERSION     DASRJB010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284777"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VFB80_16595"
     CDS             complement(<1..1740)
                     /locus_tag="VFB80_16595"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN35450.1"
                     /translation="MTRALSAALALVATAALAADPSMNSLAPAGVQRGTETEFTITGG
                     NLADVQEILFYTPGFTVKKLTPADNNLKALIAVAPDCQLGIHAIRIRSLGGVSNLRTF
                     TVGNLPEVKEIEPNTLFTQPQAISPNVTVSGTIQNEDVDHFVVELKKGDRLTAELEGL
                     RLGNTFFDPYVAILNAERFELTRSDDAALLNQDCLASIVAQEDGKYIVQVRESSFGAG
                     SAYRLHIGTFPRPTAAFPPGGRPGETLMVRWIGDAAGPFDQQITLPAANAAGEAPIVP
                     RDAKGEAPSPNVLRVIDLPGYIEAEPNDEAKAASSSGAAPLALNGIIDKENDIDFFKF
                     TAKKGQQLDVRVYARRPLRSPLDSVLTVHNAQGGGIGNNDDTGGPDSYFRFNVPADAE
                     YLVSVRDQLKAGGPDYVYRVEITENKPSLVMRLPERRQYVSTTLVVPKGNRNAVMVAA
                     QRQNFGGDLAVTFEGLPPGMTAEAIPLTAGLSEIPVLFTAAADSQPAGALVGIVGKTV
                     DEKLGIVGRLDQRTMLVRGQNNTDVWGHNADRMASVLSEEIPYSIEIVPPKAPLVRNG
                     SLDLKVVAKRKEGF"
     gene            complement(1909..>2093)
                     /locus_tag="VFB80_16600"
     CDS             complement(1909..>2093)
                     /locus_tag="VFB80_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35451.1"
                     /translation="IYGTSNATASEPDSDPVGPEDLATTVYHQLGIVADKELMAPGGR
                     PIEIVDGGKVVKGLLA"
BASE COUNT          318 a          672 c          717 g          386 t
ORIGIN      
        1 gaagccttcc ttccgcttgg cgacgacctt caagtcgagc gagccgttgc ggaccagcgg
       61 cgctttgggc gggacgatct cgatcgagta cggaatctcc tcggagagga cggacgccat
      121 ccggtcggca ttgtggcccc aaacgtcagt gttgttctgg ccgcggacga gcatcgtccg
      181 ctgatcgagc cggccgacga tgccgagctt ttcatcaacc gtcttgccga cgattccgac
      241 cagcgccccg gccggctgcg agtcggccgc ggcagtgaac aggaccggta tttccgacag
      301 gccggcggtc agcgggatgg cctcggcggt catgcccggc ggcagcccct cgaaggtcac
      361 ggccaggtcc ccgccaaaat tctgccgctg ggcggccacc atcaccgcgt tgcgattgcc
      421 cttcggcacg acaagcgtcg tcgagacata ttgccgccgc tcgggcagcc gcatgacgag
      481 cgagggcttg ttctcggtga tctcgacgcg gtagacgtaa tccggtccac ccgccttgag
      541 ctggtcgcgg acggaaacga ggtattcggc gtcggcgggc acgttgaagc ggaagtagct
      601 gtcgggaccg ccggtgtcgt cgttgttgcc gatgccgccg ccttgcgcgt tatgcaccgt
      661 cagcaccgag tcgagcggcg agcggagcgg cctacgggca tacacccgca cgtcgagctg
      721 ctgacccttc ttggccgtga acttgaagaa gtcgatgtcg ttttccttat cgatgatgcc
      781 attgagggcc agcggggcgg cacccgacga actggcggcc ttcgcttcgt cgttcggctc
      841 ggcttcgata tagcccggca ggtcgattac gcggaggacg ttcggcgacg gcgcctcgcc
      901 cttggcatca cggggtacaa tcggcgcctc gccggccgcg ttggccgccg gcaaggtgat
      961 ctgctgatcg aacgggccgg ccgcatcgcc gatccagcgg accatcagcg tctcgcccgg
     1021 ccgtccgccc ggcggaaacg ccgccgtggg ccgcgggaac gtaccgatgt gcaggcgata
     1081 ggccgagccc gcaccgaagc tgctctcgcg gacctgcacg atgtacttgc cgtcctcctg
     1141 ggccacgatc gaggccaggc agtcttgatt gagcagcgcc gcgtcgtcgc tgcgggtcag
     1201 ctcgaaccgc tcggcgttca ggatcgcgac atacggatcg aaaaacgtgt tgcccagccg
     1261 caggccctcc agctccgccg tcagccggtc ccccttcttc agctcgacga cgaagtgatc
     1321 gacgtcctcg ttctgaatcg tgccgctgac ggtcacgttt ggcgagatcg cctgcggctg
     1381 cgtgaagagc gtgttcggct cgatctcttt gacttcgggc aggttgccga cggtgaaggt
     1441 ccgcaggttg ctgacgccgc ccagcgagcg aatgcggatc gcatggatgc cgagctgaca
     1501 atcgggggcg acggcgatca gggccttgag gttgttatcg gccggcgtga gcttcttgac
     1561 cgtgaagccg ggcgtgtaga agaggatctc ctgcacgtcc gccagattgc cgccggtgat
     1621 cgtgaattcc gtttccgtgc cgcgctgcac gcccgccggg gcgaggctgt tcatgctcgg
     1681 gtcggcggcg agcgcggcgg tggcgacgag cgcgagcgca gcggacaggg cgcgcgtcat
     1741 agtggcctct caatgttgcc gctggcgggg cttcggcctc atgccgaagg agcggcgcgg
     1801 tgggcgtggg gcgggcctct tgcccgcgcg cttcgtagag caagcgtcct cgcttgctca
     1861 tggttgactc tcgtccgcgc tgcacaagcg aggacgctta tgctattgct acgccagcag
     1921 tcccttgacg accttgccgc catcgacgat ctcgatgggg cggccgccgg gagccatcag
     1981 ctctttatcg gcgacgattc cgagctggtg ataaaccgtc gtggccaggt cttcggggcc
     2041 gaccggatcg ctgtcgggct cggacgcggt cgcgttcgac gtgccgtaga tca
//
LOCUS       DASRJB010000625         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1871671, whole genome shotgun sequence.
ACCESSION   DASRJB010000625 DASRJB010000000
VERSION     DASRJB010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871671"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFB80_16605"
     CDS             <1..1164
                     /locus_tag="VFB80_16605"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35452.1"
                     /translation="GSAYLYDRKRVDALNKDFDARTKELQARRPVDDFVPCLYETPGK
                     IPQTVLFFRGDIQQPRQAVEPSELSVLTTAAAMIPQDDPALPTSGRRLAYARWLTSGQ
                     QPLTPRVLVNRFWLHHFGRGIVATPGDFGVLGARPSHPELLDWLAARFIDSGWELKRL
                     HRLLMSSTVYQQQSRRTNELDQADPENQLLGRMSIRRLEAEAVRDAMLAAAGSLNLKM
                     QGPPVPVTVDDVGQVIVGLDNRDTAGRPMGKRGVLGADEFRRSVYIQVRRSLPLSMLE
                     TFDAPLLNPNCELRTRSTVAPQSLLLMNSEFVLGQSRTLAARAQSASSDRSEQIRLLC
                     RWALAAEPTDEEISRAAAFVAALEAELAENNKDQALAGLCQALFSSSAFLYVD"
     gene            1310..3340
                     /locus_tag="VFB80_16610"
     CDS             1310..3340
                     /locus_tag="VFB80_16610"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN35453.1"
                     /translation="MGGHRFQRFIRCVALLAAALGAAFQTASAQDASVDESFFAKSLY
                     PVLHAAQCVRCHGKSGVASETRLAFPRDGASPEQVTAFGLSLALLVDRKNPEQSLLLQ
                     KPTRRIKHTGGQRIKPDSDEERALVAWINYLAGLSDEQLQKNRERIAAALPRGERMPT
                     VRRLTHSQYDHTVRDLLGDQIQPASSFPKEDFISGFKNQVEGQGISPLQAEAYGLAAE
                     RLARAAFRGGDQRGLVPGQPASPTDAQLAEKFVRQFGLKAFRRPLAENEVRLYRELFL
                     REAGRTQDFYSGATIVVEAMLQSPHFLFRAERGAENAPYEVASRLSYFLWDTMPDAEL
                     FQAAERGELASREQVERAARRMLTEARATASLEEFLAQWMRFDRVLGATRDRRRFREF
                     GTELAAAMAEETRRLFNHLVWDDQNFMEFFTADYTFVSSDLARLYGLPAPTEEFARVS
                     YPPDSGRSGVLGHGSFLVLTSKPAETSPTARGLFIRNQFLGHEVPPPPPGVNTVLPAV
                     TENAPLTNRQRLETHLNSEACANCHRLIDPIGLAFEQYNAIGVFQKKLQLQFGGGRGQ
                     GNRDRQPTMIELDLDTAGHIQGIENSAFSTPKELGKLLAESPACQKCIVKQLFRYALG
                     REETPQDQPVLESLLVRFRDSGFRFRELIVGLVTSDSFLGAAPPAPSPPAPG"
     gene            3363..4670
                     /locus_tag="VFB80_16615"
     CDS             3363..4670
                     /locus_tag="VFB80_16615"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN35454.1"
                     /translation="MLTSSQELSRRGFLRGVGLSGAAICVGLPAFESMFNAGGTAYAA
                     EAGVDPIETRFIFWFNGNGIIEKYWIPLEDGEDFALTPCLTPLARFRQDIHVLSGVDN
                     PAARLPGPGNGHHNSMSGLVAGEMFSGRGAGGASIDQAIAQKIGNNSRFRSLQIGVCQ
                     ESFGESIQRNMSWAGRDRPLPPEMIPHRLFDRLFGSKEQYWIERKKSILDAVSGEAKD
                     LKKALGRGDQQRLEEYLNSIRSLERSVASLPPEYAQVVEQPPEGGDLKDWPRIAKLQT
                     DLLVHALASRQTRVASYMLTKCQGLSRFPWLGYTYERHHEYTHGRVESPEGQRIMRDI
                     NRWHVEEFAYLLGKLKSIPEGSGTLLDHTCALFVHEHAEANSHKNAGLAMIAAGHAGQ
                     LKTGRHTRLTGTVGDLYLTLAEEVVGAKLDSFPTADKKLTEIV"
     gene            4741..5217
                     /locus_tag="VFB80_16620"
     CDS             4741..5217
                     /locus_tag="VFB80_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35455.1"
                     /translation="MNQWVDISFDCLPLRSVGRLDIPLDASPKYRQRCERIKRFLEKH
                     GSHNTYYLYNAGCAFHLTNRPEQGSLEFRFDGTVITDAADMRATAADLEVELIRETCD
                     WLTAPVVQWFAQSVQHAVCVEFDRYIEAGDLAKTKARMERLQAELEKTGGHVGMYL"
     gene            5347..6630
                     /gene="hemL"
                     /locus_tag="VFB80_16625"
     CDS             5347..6630
                     /gene="hemL"
                     /locus_tag="VFB80_16625"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZN35456.1"
                     /translation="MGRARSHEIFRRAQELMPGGVNSPARAFGGVGGEPIVFERASGA
                     YLFDVDGNRYIDYIGSWGPMILGHQHPRVVAAVQEAVGRAFTFGAPTPAENELAELIV
                     AAVPSIQMLRLVNSGTEATMSAIRLARGFTGRDAIIKFAGNYHGHVDSLLVAAGSSAA
                     TLGVPNSPGVTAGMTKDTLICRYNDSAGLARVFAEHGPRIAGVIFEPIVGNMGCVVPT
                     SEFLRALRELTAKHGALLICDEVMTGFRVAYGGAQALLKIQPDITTLGKIVGGGMPIG
                     AYGGRREIMRHVLPAGKVFQAGTLSGNPVACAAGIATLQLLRDHPPYERLEALSARLE
                     QGLHAAASRADLPHSITRVGSMLTLFFNQERPADWEGASRSDTARYARYFWGLIDRGV
                     YMPCSQYEALFVSAAHTEADIDATIQAAGEVFAEL"
BASE COUNT         1197 a         2310 c         2292 g         1141 t
ORIGIN      
        1 ggcagcgcct atctctacga ccgcaagcgg gtcgatgccc tcaacaagga cttcgacgcc
       61 cgcaccaagg agttgcaagc ccggcggccg gtcgacgact tcgtgccctg cctgtacgag
      121 accccgggca agattcccca gaccgtcttg tttttccgcg gcgacatcca gcagccgcgg
      181 caggcggtgg agccgagcga gctctcggtg ctgactaccg cggccgccat gatcccgcag
      241 gacgatccgg ccctgccgac ttctggccgc cggctggcct atgcccgctg gctgacgagt
      301 ggccagcagc cgctgacgcc gcgcgtgctg gtgaatcgct tctggctcca ccatttcggc
      361 cgcgggatcg tggccacgcc gggcgatttc ggcgtgctcg gcgcgcggcc cagccatcca
      421 gaactgctcg actggttggc agcgcggttc atcgacagcg gctgggagct gaagcggctg
      481 caccgcttgc tgatgtcttc gacggtctat caacagcagt cgcggcgcac gaatgaactg
      541 gatcaggccg atccagagaa ccagctgctc ggccggatgt cgatccgccg cctggaagcc
      601 gaggcggtcc gcgacgcgat gctggccgcg gccggctcgc tgaacctgaa gatgcagggc
      661 ccgccggtgc ctgtcaccgt cgatgacgtg ggccaggtaa tcgttggcct ggacaatcgc
      721 gatactgccg gccggccgat ggggaagcgc ggcgtgctgg gcgcggatga gttccgccgc
      781 agcgtctaca tccaggtgcg gcggtcgctg ccgctgtcga tgctggagac cttcgacgct
      841 ccgctgctga atcccaactg cgagctccgc acccgctcga cggtcgcccc gcagtcgctg
      901 ctgctcatga acagcgaatt cgtcctcggc cagtcgcgga ctctcgccgc gcgggcccag
      961 tctgcgtcga gcgaccgctc cgagcaaatc cgcctcctct gtcgctgggc gctggccgcg
     1021 gaacccaccg atgaggaaat ctccagagcg gctgcctttg tggccgcatt ggaagccgag
     1081 ctcgccgaga acaacaagga ccaggccctg gccggcttgt gccaggctct gttcagctcc
     1141 agcgcgtttc tgtacgtcga ttgacgccaa gaccctcgaa caaccggcgg cgaaatggca
     1201 tccgcgatgc gcgcccagac tgttccgaga gtgggagtaa tcgttgtcgg ctgccggagc
     1261 gaagtgctag gataggctgg tcggttgcag cagcagcagg aaggtcttca tgggcgggca
     1321 tcgattccag cgctttattc ggtgcgtcgc gctgctggcg gctgccctcg gcgccgcttt
     1381 ccagacggcc tctgcgcagg atgcctccgt cgatgagtcg ttctttgcga agtcgctcta
     1441 tccggttttg cacgccgccc agtgcgtgcg ctgccacggc aaaagcggcg tagcctccga
     1501 gacgcggctg gcgtttccgc gcgacggggc cagtcccgag caggtcacgg ccttcggcct
     1561 gtcgctcgcc ctgctggtcg atcgcaagaa tcccgagcag tcgctgctcc tgcaaaagcc
     1621 cacgcggcgc atcaagcaca ccgggggcca gcggatcaag ccggacagcg acgaagagcg
     1681 ggcgctggtg gcctggatca actacctggc cggcctgtcc gacgagcagc tgcaaaagaa
     1741 tcgcgagaga atcgctgctg ctttgccgcg cggcgagcgc atgccgaccg tccggcggct
     1801 gacgcacagc cagtacgacc acacggtgcg cgacctgctc ggcgaccaga ttcagccagc
     1861 gagcagtttt cccaaagagg atttcatcag cggctttaag aaccaggtcg aggggcaagg
     1921 gatctcgccg ctccaggccg aggcgtacgg tctcgcagcc gagcggctgg cccgggcggc
     1981 ttttcgcgga ggcgaccagc ggggcctggt tccaggccag ccggcgtcgc cgaccgacgc
     2041 gcagctcgcg gaaaagttcg tccggcagtt cggcctcaag gcgtttcgcc ggccgctggc
     2101 ggaaaacgaa gtccggctgt accgcgagtt gttcctccgc gaagcgggcc gcacgcagga
     2161 cttctacagc ggtgcaacca ttgtcgtcga ggcgatgctc cagtcccccc acttcctgtt
     2221 tcgcgcggag cggggcgctg aaaacgcccc gtacgaagtc gccagccggc tgtcgtattt
     2281 tctgtgggac acaatgcctg acgccgagct gttccaggcc gccgagcgcg gcgaattggc
     2341 ctcgcgcgag caggtcgagc gtgccgcccg gcgaatgctg acggaagcgc gggccacagc
     2401 gtcgctggaa gagttcctgg cccagtggat gcgttttgac cgcgtgctgg gagcgacccg
     2461 cgaccgccgc cggttccgcg aattcggcac cgagttggcg gccgcgatgg ctgaagagac
     2521 gcggcggctt ttcaaccacc tggtctggga cgaccagaac ttcatggagt tcttcaccgc
     2581 cgactacacg ttcgtcagca gcgacctggc ccggctctac ggcctgcctg cgcccaccga
     2641 ggaattcgcc cgcgtcagct atccgcccga tagcggccgc agcggcgtcc tgggacacgg
     2701 cagcttcctg gtactcacca gcaaaccggc ggaaacgtcg ccgactgcgc ggggcctctt
     2761 catccgcaac cagtttctgg ggcacgaggt cccgccgcct ccgccaggcg tgaacaccgt
     2821 cctgcccgcc gtcaccgaaa acgctccgct caccaaccgc cagcggctgg agacgcacct
     2881 caatagcgag gcctgtgcta actgtcaccg gctgatcgac ccgatcggcc tggccttcga
     2941 gcaatacaac gcgatcggag tgtttcagaa gaagcttcag ctgcaattcg gcggcggccg
     3001 aggtcaaggc aatcgcgacc ggcagccgac gatgattgaa ttggacctcg acaccgccgg
     3061 ccacattcaa ggcatcgaga actcggcgtt ttccacgccc aaggagttgg gtaagctgtt
     3121 ggcggaaagc ccggcctgcc agaagtgcat cgtcaagcag ctgttccgct acgcgctggg
     3181 ccgcgaagag acgccgcagg atcagccggt gctcgagtcg ctcctggtga gattccggga
     3241 ttccggattc cggtttcgcg aattgatcgt gggtttggta acatcggatt cgttcctcgg
     3301 ggccgcccca cccgcgccgt cgcctccggc tccgggttaa acgattgcag aggagatcca
     3361 ccatgttgac ctccagccag gaattgtccc gccgcgggtt tctgcggggc gtggggttgt
     3421 cgggcgcggc gatctgcgtc ggcctgccgg ccttcgagag catgttcaac gccggcggca
     3481 cggcctacgc cgccgaagcc ggcgtggatc cgatcgaaac ccgcttcatc ttctggttca
     3541 acggcaacgg gatcatcgag aagtattgga ttccgctcga ggacggcgag gactttgccc
     3601 tgacgccctg cctgacgccc ctggcccgct ttcgccagga catccacgtg ctcagtggcg
     3661 tcgataatcc ggcggcccgc ctgcccgggc cgggcaacgg ccatcacaac tcgatgagcg
     3721 gcctagtcgc cggcgagatg ttctccggcc gcggcgccgg cggagcgtcc atcgatcagg
     3781 ccatcgcgca gaagatcggc aacaacagcc gcttccgctc gctgcagatc ggcgtctgcc
     3841 aggagtcgtt cggcgagagc atccagcgga acatgagttg ggccggccgc gaccggccgc
     3901 tgccgccgga aatgattccg caccggctgt tcgaccggct gttcggctcc aaggagcagt
     3961 actggatcga gcggaagaag agcatcctcg atgcggtgag cggcgaagcc aaggacctga
     4021 aaaaggccct cggtcgcggc gatcagcagc ggctggagga atatctcaac tcgatccgca
     4081 gcctggagcg gagcgtcgcc agcctgccgc cggaatacgc gcaagtcgtc gagcagccgc
     4141 cggagggcgg cgacctgaag gactggccgc ggattgcgaa gctgcagacg gatttgctgg
     4201 tgcacgccct ggcctcgcgg cagacgcggg tcgccagcta catgctgacg aagtgccagg
     4261 gcctgtcgcg gtttccctgg ctgggctaca cctacgagcg gcaccacgaa tacacgcacg
     4321 gtcgcgtcga atcgcccgag gggcagcgga tcatgcggga catcaaccgc tggcacgtcg
     4381 aggaatttgc ctacctgctg ggcaagctca agtcgatccc cgaaggcagc ggcacgctgc
     4441 tggaccatac ctgtgccctg ttcgtgcacg agcacgccga ggcgaactcg cacaagaacg
     4501 ccggcctggc gatgatcgcc gcgggccacg cgggccagct gaagaccggc cggcacaccc
     4561 gcctcaccgg caccgtcggc gacctgtacc tgacgctggc cgaggaagtc gtcggcgcga
     4621 agctcgacag cttccccacg gccgacaaga agctgaccga aatcgtgtga tccgccgggc
     4681 cggcgctctc gccaaaagac cgcattccag tctggcaggc cggcggccta caatgggcgc
     4741 atgaaccagt gggtcgatat ttcgttcgat tgcctgccgc tgcggagcgt cggccgcctg
     4801 gatattccgc tcgacgcctc gcccaaatac cgccagcggt gcgagcggat caagcggttc
     4861 ctggaaaagc acggctcgca caacacgtat tatctctaca acgccgggtg cgcgtttcac
     4921 ctgacgaacc ggccggagca gggctcgctg gaattccgct ttgacggcac cgtcatcacc
     4981 gacgcggccg acatgcgggc gaccgccgcc gacctggagg tcgagctgat ccgcgaaacg
     5041 tgcgactggc tgaccgcgcc cgtcgtgcag tggttcgccc agtccgtgca gcacgcggtc
     5101 tgcgtcgagt tcgaccgcta tatcgaggcc ggcgacctgg ccaagaccaa ggcccggatg
     5161 gagcggctgc aagccgagtt ggagaaaacg ggcgggcacg tggggatgta tttgtaggtg
     5221 agtggtgagt acttgtaggg tgggtcgagg ccgctgcggc cgctgaccca cgcgggaaat
     5281 cgatgccgcg tgggtcagcg ctcgcggact cgcttgaccc accctaccgt gaatggatag
     5341 ggcagcatgg gccgagcgcg tagtcacgag attttccggc gggcgcagga actgatgccg
     5401 gggggcgtca acagcccggc gcgggccttt ggcggcgtcg gcggcgagcc gatcgtattc
     5461 gagcgggcca gcggcgcgta cctcttcgac gtcgacggca atcgctacat cgactacatc
     5521 ggctcctggg ggccgatgat cctggggcac cagcatccgc gagtcgtggc cgcggtgcag
     5581 gaagcggtcg gccgggcctt caccttcggc gcgccgacgc cggccgagaa cgagctggcc
     5641 gagctgatcg tggccgccgt gccgtcgatc cagatgctgc ggctggtcaa ttcgggcacc
     5701 gaagcgacga tgagcgcgat ccgcctggcc cgcgggttta cgggccgcga tgccatcatc
     5761 aagttcgccg gcaactacca cggccacgtc gatagcctgc tggtcgccgc gggcagctcg
     5821 gccgcgacgc tgggcgtgcc caattcgccg ggcgtgaccg ccggcatgac caaggacacg
     5881 ctgatctgcc gctacaacga ctccgcgggc ctggcgcggg tctttgccga gcacggcccg
     5941 cggatcgccg gcgtaatttt cgagccgatc gtcggcaaca tgggttgcgt cgtccccact
     6001 tccgaattcc tgcgggccct gcgggagctg acggcgaagc acggggcgct cttaatctgc
     6061 gacgaagtga tgacggggtt ccgcgtggcg tacggcgggg cccaagcgct gctcaaaatc
     6121 cagcccgaca tcacgacgct gggcaagatc gtcggcgggg gcatgccaat cggcgcctat
     6181 ggcgggcggc gcgagatcat gcggcacgtc ttgcccgcgg gcaaagtctt ccaggccggc
     6241 acgctcagcg gcaatccggt cgcctgcgcc gctgggatcg ctacgcttca gcttctccgc
     6301 gaccacccgc cctacgagcg gctggaagcc ctctccgccc ggctggagca aggtctgcat
     6361 gcggcggcca gccgagcgga tttgccgcac tcgatcaccc gcgtcggcag catgctcacg
     6421 ctgttcttca accaggagcg gcccgccgat tgggaaggcg ctagccgcag cgacaccgcc
     6481 cgctacgccc gctacttctg gggcctcatc gaccgcggcg tctacatgcc ctgcagccag
     6541 tacgaagccc tcttcgtgtc cgccgcccac accgaagccg acatcgacgc gacaatccaa
     6601 gccgcggggg aagttttcgc cgagctgtaa tccacccggt gctgccgcgg ccagtgttgc
     6661 cgcgcggcca taagtgaaca ttggtacgat atttgcagta tcgttccgat tggcgcgcga
     6721 gcggtcaatt tggcagcctg actgaaaaca ctcgaagctt tcgtccgtcg cccaagggca
     6781 ccacgcgccc ccaaagacaa accagcgggg aggtaaggat aattcccgcc aggggtctct
     6841 aaggcttcaa acccaaatgt ccacatatgc tgttatgatt caactcaagt catggcgcgg
     6901 caaggcgttg ggccgaggcg cggcccaatg gggcgttgtt
//
LOCUS       DASRJB010000626         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1871791, whole genome shotgun sequence.
ACCESSION   DASRJB010000626 DASRJB010000000
VERSION     DASRJB010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871791"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFB80_16630"
     CDS             <1..232
                     /locus_tag="VFB80_16630"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35457.1"
                     /translation="STVLSGGGIRGGQAIGRTDRDAAEVVERPISVTDFLATVCTILK
                     IDYKRQNRAPGIERPIPIVDTSKPIEIITELL"
     gene            complement(915..2018)
                     /locus_tag="VFB80_16635"
     CDS             complement(915..2018)
                     /locus_tag="VFB80_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN35458.1"
                     /translation="MTSTSPPSPPPFSLAEARGIVGDLFAPRPWIYWADFLASIVTGY
                     AAFGLTRWLHDIRLEPLWLRLLLQAATFSVQCACFYRAVMFNHEIAHLPERRFRAFRV
                     VWNLLCGIPFLVPSFTYATHLEHHRRHLYGTRDDGEYLPLASLSPWYFVLYLSQCLWI
                     APLAVVRFGLLTPLAWLSPRLRRLIDQRASSLVMDARHLRPLPTAAERWPSRLQELGC
                     LLYLAGCLTVPPLVWNRPLIVLAIHAYVTSVVLVLFNSLRTLAAHRYTSDGHEETFLE
                     QLLDSITLDNDSLAAVIVNPVGLRYHSTHHLFPSLPYHNLRAAHRRLLEQLPADSPYR
                     LTVERSLLKVIGDLWRHATARAGSESQVATAYR"
BASE COUNT          419 a          641 c          730 g          364 t
ORIGIN      
        1 gagcaccgtg ctctccggcg gcggcattcg cggcggtcag gcgatcggcc gcaccgatcg
       61 tgacgccgcc gaagtcgtcg agcgtccgat cagcgtcacc gatttcctgg cgacagtctg
      121 cacgatcctc aagatcgact acaagcgcca gaaccgcgct cccggcatcg agcggccgat
      181 tccgatcgtc gatacgagca agccgatcga gatcattacg gagctgctct agcggcttcc
      241 cgatcgctcg acacgcgccg cggtcgcgga ttcttcgccg ggtcgggcag cttggtggcg
      301 agtaattcgg ttgcagttgg tgtgattcgc aggcttttcc gcgttgttgg ccgcgaatta
      361 ttcgccaatc tgcctcgata tttggggatc tggcgattaa tgcactgccg ccggccgcaa
      421 gtgccttgcg gcatcagcag ttacgagcga attgagggcg gcgaacaatt cgccgcctgt
      481 cggccatggc ggcatcatct tgcgaggaac tcgttttgaa acacgaacca ggagcacgaa
      541 ggcgcgcctc atctgcttcg tgatctttgt gtttcattgc ctgcgactgt gcaactgtgg
      601 gacgccggcg ttcttcaccg ggtggcgagc cgagcctccg tggtgaaaaa ctcggacgcg
      661 aattgcgata cctggggatc tggtgaataa ttcgcgccga acggcgtaag tggctctcgc
      721 cgctggactt gaatttttca ccatggcgaa aaattcggaa tgccggaggc tggggccctt
      781 gcctgtggcc acgcgacctg gacgaccaaa ttgccaatgc cggtgttcgc cgctgcgggc
      841 tggccgcggt ttggaattgt aaacaagtgt gaagtacgtc taccaaaatg agatagctga
      901 gacctctgcc cgctctagcg gtacgcggtg gcgacctgcg actcgctgcc cgctcgggcc
      961 gtcgcgtgac gccaaaggtc gccgatcact ttcaagagcg agcgctcgac cgtcagccga
     1021 tacggcgaat cggcaggcag ctgctccagc agccggcggt gggcggcgcg caggttgtga
     1081 taggggagcg acgggaagag gtggtgcgtc gagtggtagc ggaggccgac cgggttgacg
     1141 atgacggctg ccagcgagtc gttgtcgagc gtgatcgaat cgagcagctg ctcgaggaat
     1201 gtctcttcgt ggccgtcgct ggtatagcgg tgggcggcca gcgtgcggag cgagttgaag
     1261 aggaccagca cgacgctcgt cacgtaggcg tgaatcgcca gtacgatgag cgggcgattc
     1321 cagaccagcg gtggtacggt gaggcagccc gccaggtaca gcaggcagcc cagctcctgc
     1381 aggcggcttg gccagcgctc ggcggcggtc ggcagcggcc gcagatggcg cgcgtccatc
     1441 acgagcgacg aagcccgctg gtcgattaac cgccgcagcc gcgggctaag ccaggccagc
     1501 ggcgtcaaga gcccgaagcg gaccaccgcc aggggagcaa tccacaggca ttgcgagagg
     1561 tacagcacga agtaccacgg cgagaggcta gccagcggca ggtactcccc gtcgtcccgc
     1621 gtgccataga ggtgccgccg gtgatgttcc aggtgcgtgg cgtaagtgaa cgacggaacg
     1681 agaaacggaa ttccgcagag caggttccag acgacgcgaa acgcgcgaaa tctccgctcc
     1741 ggaagatgcg cgatctcgtg gttgaacatg accgcccggt agaagcacgc gcattggacg
     1801 gaaaacgtcg ctgcctgcaa gagcagccgc agccagagcg gctccaggcg gatgtcatgg
     1861 agccagcgcg tcaggccgaa ggcggcgtag ccggtgacga tcgacgccag aaagtcggcc
     1921 caatagatcc acggcctcgg cgcgaacagg tcgccgacga tcccgcgggc ctcggcaagc
     1981 gaaaacggcg gcgggctggg cgggctggtg gacgtcatgg tgaaaccgat gcttcggcga
     2041 atcatatgcc gctgcaccgg ttgcagccca tgccaaaagt aggttcctca cgccgtgagg
     2101 aacccgcacc gccgcgggcc ttcccaccta gatgattccc gcaaacgggc gacc
//
LOCUS       DASRJB010000627         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_468063, whole genome shotgun sequence.
ACCESSION   DASRJB010000627 DASRJB010000000
VERSION     DASRJB010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468063"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VFB80_16640"
     CDS             <1..2035
                     /locus_tag="VFB80_16640"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35459.1"
                     /translation="CQCHTHKFDPIPHREYYQLFAFFNNGTDVNNKGATVSVARGELF
                     GPPPSAPPATKAPDPARLAVRQKAWEKFELARLEKLASQPQSPPAWSPAKYVEHGTQS
                     GAALELQEDNSLLASDKTSEHDVYRVVVKAGLPQIAAIRLRTLTHPSLPKNGPGLASN
                     GNFVLTEFAASTGGKELPLAAAFADHEQPSFAVGGAIDDDPKSGWAINVGPSSKARMN
                     ADHEAVFVLAQPIELKDDQTLELKLLHELNANYLVGRFAIDFSATPPPQQAALNESLL
                     AALRTAAGERTPEQVKAIADAYALTGVPTESSAGPAPAVEKAEVMVWKELEQPRPTFI
                     CLRGDFLRPDKEVGPLVPDVLSAVPPKLSPAESRSRLDLARWLVNPENPLTPRVTMNR
                     IWMRYFGRGIVETEEDFGTQGTMPTHPELLDWLAGELHRQRWSLKAMHKLIVTSSTYR
                     QASNFRPDLKDKDPRNLLLARQERLRFDAEIVRDAALSASGLLDRTIGGPSVRPPQPD
                     GVYAFTQTAKKWTPDTGPARYRRAMYTTFFRSSPHPLFTTFDAPDFQTVCTRRGRSNT
                     PLQALTVANDVAFLEFAQGLAARVQREIPLDDADARLRRAFLLALCREPSSAEFTILR
                     GYLDRQAADLAKDEERAKALLPPETKSDRVAESAALVLACRAILNTDGFITRE"
     gene            2068..3522
                     /locus_tag="VFB80_16645"
     CDS             2068..3522
                     /locus_tag="VFB80_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35460.1"
                     /translation="MPNLKSEILNLKFQTRRHFFGQCAVGVGATALNLMLQQDGLAGK
                     PVKLDPANPLDPRQPHFAPKAKNVIYLFMAGAPSQLELFDDKPMLRELHGQPPPPSLL
                     EGKRFAFLKGNETLMGSQRKFERVGECGLTLSEMLPNFRRIVDEVCWLQGLTTDVFNH
                     GPAKLFMNTGFPAPGRPALGAWAIYGLGSESRSLPGFVVLQSGPRGPRAGASLWASGF
                     LPTSFQGVPFRSKGDAILNLKSPQGLTPAAERDFYDTVGQLNQQRLGQTGDPEILTRI
                     NAYEMAFRMQTSSPELMDLSQESQETLDLYGATAGQPSYAVNCLLARRLIERGVRFVQ
                     LYHTNWDSHGGPGENLNEDFEKVCKDVDQATAALVLDLKRRGLLKDTLVIWGGEFGRT
                     PMGEVRNTIGRDHHIDAFTMFLAGAGVKPGLIHGKTDELGFGVVEDKHHVHDLHATIL
                     HLLGFDHEQLTYRFQGRDFRLTDVHGHLIEKILT"
     gene            complement(3519..>3721)
                     /locus_tag="VFB80_16650"
     CDS             complement(3519..>3721)
                     /locus_tag="VFB80_16650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZN35461.1"
                     /translation="TACHEIGHLLGLRHGGPDEIMAASYRGQRKIPNEVDAKKAQHFW
                     GPAPPPMGTPTDQGGFGSGSGT"
BASE COUNT          652 a         1302 c         1177 g          590 t
ORIGIN      
        1 ctgccagtgc cacacgcaca agttcgaccc gatcccgcac cgcgagtact accagctttt
       61 cgcgtttttc aataatggca ccgacgtcaa caacaaaggc gcgacggtct ccgtggcccg
      121 cggcgaactg ttcggcccgc cgccgagcgc cccgcctgcc acgaaagcgc ccgatcccgc
      181 acgcctggct gtccggcaga aggcctggga gaagttcgag ctggcccgcc tggagaagct
      241 cgcctcgcag ccgcaatcgc ccccggcctg gtcgcctgcc aagtacgttg agcatggcac
      301 acagagcggc gcggccttgg agcttcagga ggacaactcg ctgctcgcca gcgataagac
      361 ttcggagcac gacgtctatc gcgtcgtcgt caaggctggg ctgcctcaaa tcgccgcgat
      421 ccgcctgcgg acgctgacgc atccttcgct gccgaagaac ggacccggcc tggccagcaa
      481 cggcaacttc gtgctcacag aattcgcggc gagcaccggc ggtaaggagc tgccgctagc
      541 tgcggccttt gccgaccacg agcagccgag cttcgcggtc ggcggagcga tcgatgacga
      601 tccgaagtcg ggctgggcga tcaacgtcgg gccgagctcg aaggccagaa tgaacgccga
      661 tcacgaggcg gtgttcgtgc tggctcaacc gattgagttg aaggacgacc agacgctgga
      721 attgaagctg ctgcacgaac tgaatgcgaa ctacctggtc ggccgcttcg cgatcgattt
      781 ctccgccact ccgccgcctc agcaggccgc gctgaacgaa tcgctgctgg ccgcgctgcg
      841 gaccgcggcc ggcgagcgga cgcccgagca ggtcaaggcg atcgccgatg cctacgccct
      901 cacgggagtg cccaccgagt cgagcgcggg tccagcgccc gcggtcgaaa aggccgaagt
      961 gatggtctgg aaagagctgg agcagccgcg gccgactttc atttgcctcc gcggcgactt
     1021 tctgcggccg gacaaggaag tggggccgct cgtgcccgac gtactgagcg cggtcccgcc
     1081 gaagctgtcg ccggccgagt cgcgcagccg gctcgacttg gcccgctggc tggtcaatcc
     1141 cgagaacccg ctgacgccgc gggtcacgat gaaccgcatc tggatgcggt atttcggccg
     1201 cggcatcgtc gagaccgagg aggacttcgg gacgcagggg acaatgccca cgcatcccga
     1261 gctgcttgac tggctcgccg gcgagctgca ccgccagcgc tggtcgctga aggcgatgca
     1321 caagctgatc gtcaccagca gcacctatcg gcaggcctcg aacttccgcc cggacctgaa
     1381 ggacaaggat ccccgcaacc tgctgctcgc ccggcaggag cggctgcggt tcgacgccga
     1441 gattgtccgc gacgctgccc tttcggccag cggcctcttg gaccgcacga tcggcggtcc
     1501 cagcgtccgc ccgccgcagc ccgatggcgt ctatgccttc acgcagaccg cgaagaagtg
     1561 gacgcccgac accggcccgg cccgctatcg ccgggcgatg tacacgacct tcttccgcag
     1621 ctcgccgcac ccgctcttca cgacgttcga cgcgcccgat ttccagaccg tctgcacccg
     1681 ccgcggccgc agcaacacgc cgctccaggc gctcaccgtc gccaacgacg tcgcattcct
     1741 ggaatttgcc cagggactcg ctgcccgcgt gcagcgtgaa atcccactgg acgacgccga
     1801 cgcccgtctc cgccgcgcct tcctgctggc cctctgccgc gagccctcct ccgccgagtt
     1861 caccatcctc cgcggctacc tcgatcggca agccgccgac ctggcaaagg acgaggagcg
     1921 ggcgaaggcc ttgctgccgc cggagacgaa atcggatcga gtcgctgaat cggcagcctt
     1981 ggttctggcg tgtcgagcaa tcctgaatac ggatggtttc atcacgcggg aatgagcaaa
     2041 tgcccactga ctactgacaa ctgaccaatg cccaatctga aatctgaaat cctgaatctg
     2101 aaattccaaa cccgccgcca tttcttcggc cagtgcgccg tcggcgtcgg cgcgacggcg
     2161 ctcaacctga tgttgcagca ggacggcctg gcgggcaagc ccgtgaagct cgatccagcc
     2221 aacccgctcg acccgcggca gccgcatttt gcgcccaagg cgaagaacgt catctacctg
     2281 ttcatggccg gcgcgcccag ccagctggag ctcttcgacg acaagccgat gctccgcgag
     2341 ctgcacggcc agccgccgcc gccgagcctg ctggagggca agcgattcgc gttcctgaag
     2401 gggaacgaga cgctgatggg tagccagcgg aagttcgagc gggtcggcga gtgcggcctg
     2461 accttgagcg agatgctgcc gaacttccgc cggatcgtcg atgaggtctg ctggctgcag
     2521 gggctgacga ccgacgtctt caaccacggc ccggcgaagc tgttcatgaa cacgggcttt
     2581 cccgcgcccg gccggccggc gctcggggcc tgggcgatct acggcctggg cagcgaatcg
     2641 cggagtctgc ccggcttcgt cgtgctccag tcgggcccgc gcgggccgcg ggccggcgcg
     2701 tcgctgtggg ccagcggctt tctgcccacg tcgttccagg gcgtcccctt ccgcagcaag
     2761 ggcgacgcga ttctgaacct caagagcccg cagggcctga cgcccgccgc cgagcgcgac
     2821 ttttacgaca ccgtcggcca gctcaatcag cagcggctcg gccagacggg tgacccggaa
     2881 attctcacgc ggatcaacgc ctacgagatg gcgttccgca tgcagaccag cagccccgag
     2941 ctgatggacc tctcacagga gtcgcaggag acgctcgacc tgtatggcgc gacggccggc
     3001 cagccgtcct atgccgtgaa ttgcctgctg gcccgccggc tgatcgagcg gggcgtgcgg
     3061 ttcgtgcagc tctaccacac caactgggac agccacggcg gtcccggtga gaacctgaac
     3121 gaggattttg aaaaggtttg caaagacgtc gatcaggcga ccgccgcgct ggttctcgac
     3181 ctcaagcgcc gcggcctctt aaaggacacg ctggtcatct ggggcggcga gttcggccgc
     3241 acgccgatgg gcgaggtccg caacacaatc ggccgcgacc accacatcga cgccttcacg
     3301 atgttcctcg ccggcgcggg cgtgaagccc gggttgatcc acggcaagac cgacgagcta
     3361 ggcttcggcg tcgtcgagga caagcaccac gtccacgacc tgcacgccac gatcctgcac
     3421 ctcttgggct tcgaccacga gcagctcacc taccgcttcc agggccgcga cttccgcctg
     3481 accgacgtcc acggtcatct gatcgagaag atcctgacct aagtgccgct tccggagccg
     3541 aacccgccct ggtccgtcgg cgttcccatg ggtggaggcg cggggcccca aaagtgctgc
     3601 gcctttttgg cgtcaacttc gttggggatc ttgcgctggc ctcgatagct ggcggccatg
     3661 atctcgtccg gcccgccgtg tctcaggccg agcaaatggc caatctcgtg gcaggccgtc
     3721 g
//
LOCUS       DASRJB010000628         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2149382, whole genome shotgun sequence.
ACCESSION   DASRJB010000628 DASRJB010000000
VERSION     DASRJB010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149382"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFB80_16655"
     CDS             <1..778
                     /locus_tag="VFB80_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HZN35462.1"
                     /translation="EALARCGAKHLGLHQQTVCRGIEHLREELARVEALGGEGLMLRQ
                     PGSSYEVGRSATLLKVKTFHDGDARVIEHQPGAGRHKGRLGALGVELADGTRFFVGTG
                     FSDAQRANPPAIGSTITFRYQELSDRGVPRFPSFVRVRTDVAASAASGRREPPDIVPS
                     ARSASPHREAGSSRSPTKAGASRSPEAAKRYFEFSDDKSSKFWEIAVSGTSVTVRFGR
                     IGASGQTQTKSFTDEAAAQAHAARLIGDKMEKGYEEKPPS"
     gene            832..1317
                     /locus_tag="VFB80_16660"
     CDS             832..1317
                     /locus_tag="VFB80_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35463.1"
                     /translation="MSFDDQLRQAIERGQRRGDLQARQAAARALTEDEYRRLHSQARL
                     TLSDHIESCVKRLPTYFPGFQYETMYGDRGWGGACFRDDLVISRGSRASDYSRLEVTV
                     RPYSTVHVLDLAAKGTIRNREIFNRNYFEPLDQADAAKFLQLIDAWVLEYAELYAART
                     S"
     gene            1324..1740
                     /locus_tag="VFB80_16665"
     CDS             1324..1740
                     /locus_tag="VFB80_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZN35464.1"
                     /translation="MIALTNSPIDPAAVLAQVASREAGAVVLFLGTTREFTGGRRTVS
                     LDYECYPEMAERKLAELEAEARRRWPLTGCMVVHRLGHLELGEASIAIAVSSPHRRVA
                     FEAGQWLIDTIKQVVPIWKQEHWADGTSEWVHPGIS"
     gene            1737..2738
                     /gene="moaA"
                     /locus_tag="VFB80_16670"
     CDS             1737..2738
                     /gene="moaA"
                     /locus_tag="VFB80_16670"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZN35465.1"
                     /translation="MTDNSPLIDSLGRVHTNLRISVTDRCNIRCFYCMPDVNVRFLPR
                     RDILSFEEIERFARVSARLGVNKLRLTGGEPLVRAELHKLVARLAAIEGIHDIALTTN
                     GILLAEQAQALKDAGLLRINISLDALTEETFRHIARRDGLDRVLAGIHAAKRVGFQRI
                     RLNAVAIKGVTEPEVVPLANFAREHGMEMRFIEFMPLDAENHWQHDQVLTGEEIRRQL
                     EDAIGPLEPAERPDPSQPATDFRFSDGGGTIGFINPVTQPFCEDCNRLRLTAEGKVRN
                     CLFSTVEWDARAVLRGGGSDDDLIDLLRTCIRAKKPGHGIDSSDFVKPERAMYQIGG"
     gene            complement(2752..>3777)
                     /locus_tag="VFB80_16675"
     CDS             complement(2752..>3777)
                     /locus_tag="VFB80_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35466.1"
                     /translation="ELLVVIAIIGVLVALLLPAVQSAREAARRMKCQNNLRQITLASH
                     NFEDANKKFPYGTKADVLDAYHWLHLTLSYLEQKNVQDLYSNLDTPLVKPITGDWPNS
                     HGFGTSAPFRQARMTVIDVYQCPSDAPHVHNEGGNTLPYYERMRGNYRGCTGNADMYG
                     EARSPIPTTLAAGRGVFYTVPGQWMGGDPPPHYTRIAEITDGTSNTLMFAEGLKSKKS
                     IQWAGTMGDITIGNMGGAFFSAFNTPNTTAADRIWGPCPRDNVGGQIDQAYKAPCSSL
                     GGPLRPPGSSTRNQTGAHAAARSRHPNGVNVTMSDGSVRFVSQSIALATWQAMATMDL
                     GETLGEF"
BASE COUNT          655 a         1245 c         1247 g          630 t
ORIGIN      
        1 cgaagcgctc gcccgctgcg gggcaaagca cctggggctg caccagcaga cggtctgccg
       61 cggcatcgaa cacctgcgcg aggagctggc ccgcgtcgaa gcactcggcg gcgaagggct
      121 gatgctgcgg cagcccggct cgagttacga agtcggccgc tcggcgacgc tgctgaaggt
      181 caagaccttt cacgacggcg acgcccgcgt gatcgagcat cagccgggag cgggacggca
      241 caagggccgc ttgggggcgc tcggcgtgga actagccgac ggcacgcggt tctttgtcgg
      301 cacgggcttc tccgacgccc agcgggccaa cccgccggca atcggcagca cgatcacctt
      361 tcgttaccag gagctgtccg accgcggcgt gccgcggttc ccatcgttcg tccgcgtgcg
      421 gaccgacgtc gccgcgagcg cggcgagcgg gaggcgtgag cctcccgata tcgttccatc
      481 cgcacgatcg gcttcacccc atcgggaggc tggctcctcc cgctcgccga ccaaggctgg
      541 cgcctctcgc tcgccggaag cggcaaagcg ctatttcgag ttcagcgacg acaaatcgag
      601 caagttctgg gagatcgcag tcagcgggac gagcgtcacc gtccgattcg ggcggatcgg
      661 cgcaagcggc cagacccaaa ccaaatcgtt caccgacgaa gcggcggccc aggcgcacgc
      721 cgccaggttg atcggcgaca agatggaaaa gggatacgag gaaaagccgc cttcttgatg
      781 tctctggacc atacgcctgc cccgtgttcg catcggactt ataatgccag catgagtttc
      841 gacgaccagc ttcgccaggc gatcgagcgc gggcagcggc gcggcgattt gcaggcccgc
      901 caggccgcgg cccgcgcgct gaccgaggac gaatatcgcc ggctgcattc gcaagcccgg
      961 ctcacgctct cggaccacat cgaaagctgc gtcaagcggt tgccgaccta cttccccggc
     1021 tttcagtacg aaacgatgta cggcgatcgc ggctggggcg gggcctgctt tcgcgacgac
     1081 ctggtgatca gccgcggttc gcgggcgagc gactatagcc ggctggaagt cacggtgcgg
     1141 ccgtactcga cggtacacgt gctcgacctg gcggcgaagg ggacgatccg caaccgcgag
     1201 atcttcaacc gcaactactt cgagccgcta gaccaggccg acgccgcgaa gttcctgcag
     1261 ctgatcgatg cctgggtttt ggaatacgcc gaactgtatg ccgcgcggac ctcgtaacag
     1321 ccgatgatcg ccctgacgaa ttcgccgatc gatcctgcgg ccgtgctcgc gcaggtcgcc
     1381 agccgcgagg ccggcgcggt cgtgctgttt ttgggcacga cccgcgaatt caccggcggc
     1441 cggcggaccg tgtcgctcga ctacgaatgc tatcccgaaa tggctgagcg gaagctggcc
     1501 gaattggagg ccgaggcccg ccgccgctgg ccgctcaccg gctgcatggt cgtccaccgc
     1561 ctcggccacc tggagctggg cgaggccagc atcgccatcg ccgtcagctc gccccaccgc
     1621 cgcgtcgcct tcgaagccgg ccaatggctc attgacacca tcaaacaggt cgtgccaatc
     1681 tggaagcagg agcactgggc cgatggaacg agcgaatggg tgcatccggg aatttcatga
     1741 cagacaattc accacttatc gattcactcg gccgcgttca cacgaattta cggatcagtg
     1801 tgaccgaccg ctgcaacatc cgctgcttct attgcatgcc ggatgtgaac gtgcggttcc
     1861 tgccgcggcg cgacatcctc tcgttcgagg agatcgagcg gttcgcgcga gtcagcgcgc
     1921 ggctgggcgt gaacaagctg cggctgacgg gcggcgagcc gctggtgcgg gccgaattgc
     1981 acaagctggt cgcccggctg gcggcgatcg agggcatcca cgacatcgcc ctgaccacca
     2041 acggcatcct cctggccgag caggcccagg cgctgaagga cgccggcctg ttgcggatca
     2101 acatcagcct cgacgccctc acagaggaga ccttccgcca catcgcccgc cgcgacggcc
     2161 tggaccgcgt gctggccggc attcacgccg ccaagcgcgt cggcttccag cggattcgcc
     2221 ttaacgcggt ggcgatcaag ggcgtcaccg agccggaggt cgtgccgctg gcgaatttcg
     2281 cccgcgagca cggcatggag atgcggttca tcgagttcat gccgctggat gcggagaacc
     2341 actggcagca cgaccaggtc ctcaccggcg aggaaatccg ccgccagctc gaggacgcca
     2401 tcggcccgct ggagccggcc gagcggcccg atcccagcca gccggcgacc gacttccgct
     2461 tttccgacgg cggcggcacg atcggcttca tcaacccggt gacgcagccg ttttgcgaag
     2521 actgcaaccg cctgcggctg acggccgaag gaaaggtccg caactgcctg ttctcgacgg
     2581 tcgaatggga cgcccgcgcc gtcctccgcg gcggcggcag cgacgacgac ctgatcgact
     2641 tgctccgcac ctgcatccgc gcgaagaagc ccggccacgg catcgactcg tcggacttcg
     2701 tcaaacccga gcgggccatg taccagatcg gcggctagcc ggcggcacgg cctagaactc
     2761 gcccagcgtc tcgccgaggt ccatggtggc catggcctgc cacgtggcaa gggcgatcga
     2821 ttggctcacg aaccggacgg agccgtcgct catcgtgaca ttgacgccat tgggatgccg
     2881 gctccgggcg gcggcgtgcg cgcccgtttg gttgcgcgtg ctgctgcccg gggggcggag
     2941 cgggccgccc agcgaactgc acggcgcttt gtacgcctgg tcaatctgtc cgccgacgtt
     3001 atctcgcggg cacggtcccc aaatccggtc ggcggcggtc gtgttgggcg tattgaacgc
     3061 cgagaagaat gcgcccccca tattgccgat cgtgatgtcc cccatcgttc cggcccactg
     3121 gatcgatttc ttggacttga ggccctcggc gaacatgagc gtattcgacg tgccgtcggt
     3181 gatttcggcg attcgcgtgt agtggggcgg cggatcgccc cccatccact gaccgggcac
     3241 ggtgtagaac actccgcgac cggcggccaa cgtcgtcgga atcggcgagc gggcctcgcc
     3301 gtacatgtca gcgtttccgg tgcagccgcg atagttgccg cgcatccgct cgtagtacgg
     3361 gagcgtgttg ccgccttcgt tgtgaacatg cggcgcgtcg ctgggacatt ggtagacgtc
     3421 gattacggtc atccgggcct ggcgaaaggg ggcggacgtg ccgaacccgt gagaattggg
     3481 ccaatcgccg gtgatcggct tgacgagcgg cgtatcgaga ttgctgtaaa ggtcctggac
     3541 gttcttctgt tcgaggtatg aaagcgtcaa gtgcagccag tggtaggcat ccagcacgtc
     3601 ggccttggtg ccatacggaa acttcttgtt ggcgtcctca aagttgtggg atgccagcgt
     3661 gatctgccgc aggttgttct ggcacttcat cctccgcgca gcttcgcggg cggattgcac
     3721 cgcgggaagc aatagcgcca ccagcacacc gattatggcg attacgacca ggagctc
//
LOCUS       DASRJB010000629         6138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1033361, whole genome shotgun sequence.
ACCESSION   DASRJB010000629 DASRJB010000000
VERSION     DASRJB010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033361"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFB80_16680"
     CDS             complement(<1..552)
                     /locus_tag="VFB80_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN35467.1"
                     /translation="MTSFDSASRREFLAASTALAAAAALPALSRAQEKQEKKEQLFKI
                     SLAQWSLHRTLRAGELDNKDFAKTAKEKFGIEAIEYVNQFFKDKAKDTAYIAELKKRA
                     EDLGVKTLLIMCDGEGALGDPDAAKRTTAVENHYKWVEAAKELGCHSIRVNAQSGASL
                     PFEEQQKLAADGLRRLSEFAAPHG"
     gene            722..1561
                     /gene="pstB"
                     /locus_tag="VFB80_16685"
     CDS             722..1561
                     /gene="pstB"
                     /locus_tag="VFB80_16685"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZN35468.1"
                     /translation="MSEGGINRLEAIVRGEPIEAVVHKRVESEQPVLEINKFSLWYGS
                     KQALFDVSLSVPKGKVTAIIGPSGCGKSTLLRCVNRMNDLIDGVRTEGDMRVNGDSIY
                     AHGVDVIELRKRMGMVFQKPNPFPMSIFENVVYPLRIDGERNRKIIQAVCEQSLIGAA
                     LWDEVAGRLRESALGLSGGQQQRLCIARAIAGEPEVLLLDEPCSALDPIATDKIEDLI
                     LELRGDFSILMVTHNMQQASRVSDYTAFMYLGRLIEYGPTADIFTRPKLKETEAYVTG
                     RFG"
     gene            complement(1592..2467)
                     /gene="lpxI"
                     /locus_tag="VFB80_16690"
     CDS             complement(1592..2467)
                     /gene="lpxI"
                     /locus_tag="VFB80_16690"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913214.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HZN35469.1"
                     /translation="MIESSTPHPTPARIGLVAGWGRFPLAVARSLAAQGCEVHCVGLR
                     GLADAALADECTSFRICGLARLGAHIRCLKSCGVTRATLAGKLFKHKMLFGRMGWLSL
                     WPDWRTIRAFWPHFVLRRHDRRDDTLLAVVAAEYAKEGITMSPATDFAPHLLAQAGQL
                     TRRGPTASQRQDIEFGWTLAKEMGRLDIGQCVAVQGRAIIAVEAVEGTDACIRRAGEL
                     CPQGGFTVVKVAKPQQDMRFDVPTIGLGTIESLIAAGAGLLAIEAGKTIVVDQDQAIA
                     LADKHGLAIVALERP"
     gene            complement(2482..3543)
                     /gene="lpxD"
                     /locus_tag="VFB80_16695"
     CDS             complement(2482..3543)
                     /gene="lpxD"
                     /locus_tag="VFB80_16695"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZN35470.1"
                     /translation="MNGLALSEIARLVEGTLSGEGETLILGAATLAVARPGEITLADG
                     PKLAPQLARSQASAVIVPPNFKPEGRPYITVGDVHAAFARVVSHYRPPRKTRLSGISP
                     SAYVHPSATIGPDVEIHPHVFVGADVVIGEGSVIHSGVQILDGCNLGRHCRLFPNAVL
                     YENTVLGERVIVHAGAVLGAYGFGYTTVQGHHKLCSQLGYVEVGDEVEIGAGTAIDRG
                     TYGPTVIGAGTKIDNLVQIGHNCRIGSHNLICSQVGIAGSSTTGDYVVLAGQVGVADH
                     LHIADGALVGAKAGVMIDAAAGKRYLGAPAVEERQFFRTISLLQQLPSMRKQLAELRQ
                     QMAQLLAQAAEESPARDAA"
     gene            3805..5106
                     /locus_tag="VFB80_16700"
     CDS             3805..5106
                     /locus_tag="VFB80_16700"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZN35471.1"
                     /translation="MQVTKVALIGMGTVGSGVARLLIDHGDRTARHAGRTLWVEAVVV
                     QDVNKPRDYELPKGVLTDDLSRVLKNGEIKLAAMLVGGVEPARTITLRLLESGKDIVT
                     ANKLLLAEHGPELFDRARELGRSIAFDASVAGGIPIITNISQCFSANQIQNLRGILNG
                     TTNFIVSQMEEQGATYAAALKEAQERGYAEADPTMDVSGGDAAQKLAILAHLAFGARV
                     HWSEITRIGIDALDASDLRYAKELGYRIKLIAVAQLHAGGLELHVTPTLVKMGTPLAE
                     VRGAYNAIQAVGDAVGPVFFHGLGAGQMPTASAVVADMIDTAVGRTGITFRTLNLWSQ
                     GAARVQTLDHKQLGGRYYLRFTVEDRPGVLAEIAGVLGRHQISIASVIQHEPVGDGAG
                     RTVPLVIMTYQAKEGAAQAAMAEIDRLGCMREKSVRLRVLD"
     gene            5179..>6138
                     /locus_tag="VFB80_16705"
     CDS             5179..>6138
                     /locus_tag="VFB80_16705"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZN35472.1"
                     /translation="MKYAIVIPDGCADEPQAALGGKTPLQAARTPAMDEIARLGVVGR
                     SNNVPAHLPPGSDVANLSLFGYDPNKYFTGRAPLEAAAQGIQLGPADWAIRCNLVTIE
                     NQTMRSFTADHVSSAEAAALLKTCQEKLGSAELEFIPGVSYRNLLIYRGAKQPAPFTR
                     DTRATPPHDLTDKSVADDFPRGPGSDFLNDLMGRSVALFAEHPVNAARKAAGKLPATN
                     VWLWGLGKTPALTPFFELHKKRGKMITAVDLLRGLAALIGWERIEVPGATGYLDTDYA
                     AKGRYAVEALTTTDIVCVHVEATDEASHEGRADAKIKALEEIDA"
BASE COUNT         1058 a         2001 c         2058 g         1021 t
ORIGIN      
        1 gccgtgcggc gcggcgaatt ccgacagccg gcggagccca tccgcggcga gcttctgctg
       61 ctcctcgaag gggaggctcg cgcccgactg ggcgttgacg cggatcgagt ggcagcccag
      121 ctccttggcc gcctcgaccc atttgtagtg gttctcgacg gcggtcgtcc gcttggcggc
      181 gtccggatcg cccagggccc cttcgccgtc gcacatgatc aagagcgtct tcacgcccag
      241 gtcctcggcc cgcttcttca attcggcgat gtaggccgtg tccttggcct tgtccttgaa
      301 gaactgattc acgtattcga tggcctcgat gccgaacttc tccttggcgg tcttcgcgaa
      361 atccttgttg tcgagctcgc ccgcgcggag cgtccggtgc agcgaccact gggccagcga
      421 aatcttgaac agctgctcct tcttctcttg cttctcttgg gcccgactca acgccggcag
      481 cgccgcggca gcagccaggg ccgtggaggc ggcgaggaat tcacggcgcg aggcgctatc
      541 gaaagaggtc atggcgagat tccggagagg gcgacggggt ctgcgtaaac ggctcaatat
      601 aaccagccgc ttttcgggct gctactcgtt ccagcgggcc tgaatttcct cgattcttaa
      661 ccgcatccgc ctcgccgccg atgccagagc cgcttaggat gtcgccagaa aaggacgcgg
      721 catgagcgaa ggcgggatca accggttgga ggcgatcgtt cgcggcgagc cgatcgaggc
      781 cgtcgtccat aagcgagtcg agagcgagca gccggtgctc gaaatcaaca agttttcgct
      841 ctggtacgga tcgaagcagg ccctgttcga cgtctcgctg tccgttccca agggcaaggt
      901 cacggcgatc atcgggccgt cgggctgcgg caaatcgacc ctgctgcggt gcgtcaaccg
      961 gatgaacgac ctgatcgacg gcgtgcggac cgagggggac atgcgggtca acggcgactc
     1021 gatctatgcc cacggcgtcg atgtgatcga gctccgcaag cggatgggca tggtcttcca
     1081 gaagccgaac ccgtttccga tgagcatctt cgagaacgtc gtctatccgc tgcggatcga
     1141 cggcgagcgg aaccgcaaga tcatccaggc cgtctgcgag cagagcctca tcggggccgc
     1201 cctgtgggac gaagtcgccg gccgcctgcg cgagagcgcc ctggggcttt ccggcggaca
     1261 gcagcagcgg ctgtgcatcg cccgggcgat cgccggcgag cccgaagtgc tgctgctcga
     1321 cgagccttgc tcggctctcg acccgatcgc caccgacaag atcgaagact tgatcctcga
     1381 gctgcgcggc gacttttcga tcctgatggt gacccacaac atgcagcagg cctcgcgggt
     1441 cagcgattac acggccttca tgtacttggg ccgcctgatc gagtacggcc cgacggccga
     1501 cattttcact cgccccaagc tcaaagagac cgaagcctac gtcacgggcc gcttcggctg
     1561 accgtagccg acgcagcctg cgtcggcttt gctacggccg ctccaaagcg acgatggcca
     1621 ggccgtgctt gtcggccagg gcgatggcct ggtcttgatc gacaacaatt gtcttgccgg
     1681 cctcaatggc cagcaggccg gcgccggcgg caatgagcga ttcgatcgta cccaagccga
     1741 tcgtgggaac gtcgaaccgc atgtcctgct gcggcttggc gaccttgacg acggtgaagc
     1801 cgccctgcgg gcagagttcg ccggcgcggc ggatgcaggc gtcggtccct tcgaccgctt
     1861 cgaccgcgat gatcgcgcgg ccctgcacgg ccacgcactg gccgatgtcg aggcggccca
     1921 tttccttggc cagcgtccag ccgaactcga tgtcctgccg ctgggacgcg gtcggtccgc
     1981 ggcgggtcag ctggcccgct tgtgccagca ggtgcggagc aaaatcggtg gcgggcgaca
     2041 tggtgattcc ttccttggcg tattccgcag cgacgaccgc cagcagcgtg tcgtcgcggc
     2101 ggtcgtggcg gcggagcaca aagtgcggcc agaacgcgcg gatcgtccgc caatcgggcc
     2161 acagcgacag ccagcccatc cggccgaaga gcatcttgtg cttgaagagc ttgccggcca
     2221 gggtcgcccg ggtgacgccg caacttttga ggcagcggat atgcgctccc aggcgggcca
     2281 ggccgcagat gcgaaagctc gtgcattcgt ccgccagcgc cgcatcggcc aggccccgca
     2341 atccgacgca gtgcacctcg cagccttggg ccgcgagcga acgggcgacc gcgagtggaa
     2401 agcggcccca gccggcgacc aggccgatgc gggcaggcgt cggatgcggt gtggaggatt
     2461 caatcacggc tcaagaattt ctcatgcagc gtctcgcgcc ggagactctt ccgccgcctg
     2521 cgccagcagc tgggccatct gttgccgcag ctcggccagt tgcttgcgca tcgagggcag
     2581 ttgctggagg agcgagatgg tgcggaaaaa ttgccgctct tcgaccgccg gcgcgccgag
     2641 gtagcgcttg ccggcggccg cgtcgatcat gactccggct ttcgcgccaa ccagcgcgcc
     2701 gtcggcgata tgcaggtggt ccgcaacgcc gacctggccg gcgagcacca cgtagtcgcc
     2761 cgtagtcgag ctgccggcga tgccgacttg cgagcagatc aggttgtggc tgccgatgcg
     2821 gcagttgtgg ccgatctgca cgaggttgtc gatcttcgtc cccgcgccga tgaccgtcgg
     2881 gccgtaggtg ccgcgatcga ttgccgtccc cgcgccgatc tccacttcat cgccgacttc
     2941 gacgtagccc agctgcgagc agagcttgtg gtggccttgc acggtcgtgt agccgaagcc
     3001 gtatgctccg agcactgcgc ccgcgtgcac gatcacccgc tcgcccagca ccgtgttttc
     3061 gtagagcacc gcgttgggga acagccggca gtggcggccc aggttgcacc catcgagaat
     3121 ctgaacgccg ctgtggatga ccgagccctc gccgatcacg acgtccgcgc cgacgaacac
     3181 gtgcggatgg atttcgacgt cggggccaat cgtcgccgac ggatgcacgt aggccgaggg
     3241 gctgatcccg gagagccgcg tcttccgcgg cgggcgatag tggctgacga cgcgggcgaa
     3301 cgccgcatgg acgtcgccga cggtgatgta gggccgacct tccggcttga aattgggcgg
     3361 cacgatcacc gccgacgcct gcgagcgggc cagttgcggc gcgagcttcg gcccgtcggc
     3421 cagcgtgatc tcgcccggac gggccacggc cagcgtcgcc gcaccgagaa tcagcgtttc
     3481 gccctcgccg ctcagcgtcc cttccaccag ccgagcaatt tccgacaggg ccaggccgtt
     3541 catgcggagt cctttccgta cgccaagacg ggtagccgcc agatcgtagc cgcgcacgcg
     3601 tgtcccacca agaccgaccg ttgccggccg aacggctgag ctacggacga aacacagatt
     3661 tgcaccgctg gcgtgattgc ctgccgcttg acgttttcca aagattgcaa atagcataga
     3721 aacgcttgcg gcaggcgcga ccttcccgga tcctcacacg tcgtgccgca acccgctggc
     3781 ccgcaatact ttacgttcgc agctatgcaa gtcaccaaag tcgccctcat cggcatgggt
     3841 acggtcggct cgggcgtggc ccggctgctg atcgaccatg gcgaccggac cgcgcggcat
     3901 gcggggcgga cgctgtgggt cgaggcggtg gtcgtgcagg atgtgaacaa gccacgggat
     3961 tacgagctgc ccaagggcgt gttgaccgac gacctgtcgc gggtgctgaa gaacggcgag
     4021 atcaagctgg ccgcgatgct cgtaggcgga gtggagccgg cgcggacgat cacgctcaga
     4081 ctgctggaga gcggcaagga catcgtcacg gcgaacaagc tgctgctggc cgagcacggg
     4141 ccggaactgt tcgaccgggc gcgggagctg ggccggtcga tcgccttcga tgcctcggtg
     4201 gccggcggga ttccgatcat cacgaacatc agccagtgct tctcggccaa ccagatccag
     4261 aacctccgcg gcatcctgaa cggcacgacg aacttcatcg tctcgcagat ggaggagcag
     4321 ggcgcgacct atgcagcggc cttgaaggag gcgcaggagc gcggctatgc cgaggccgat
     4381 ccgacgatgg acgttagcgg cggcgacgcg gcccagaagc tggcgatcct ggcccacctc
     4441 gcgttcgggg cgcgggtcca ctggagcgag atcacgcgga tcggcatcga cgccctcgac
     4501 gcgtccgacc tgcggtatgc gaaggagctg ggctatcgca tcaagctgat cgccgtggcc
     4561 cagctgcacg ccggcgggct ggagctgcac gtcacgccga cgctggtgaa aatgggaact
     4621 ccgctggccg aagtccgcgg ggcctacaac gcgattcaag cggtcggcga cgcggttggc
     4681 cccgtctttt tccacggcct gggcgcgggg cagatgccga cggcgtcggc cgtcgtcgct
     4741 gacatgatcg acacggcggt cggccgcacg ggaatcacgt tccgcacgct caatctctgg
     4801 tcgcagggcg cagcccgggt ccagaccctc gatcacaagc agctcggggg ccgctattat
     4861 ctgcggttca ccgtcgagga ccggccgggt gtcctggccg agatcgccgg cgtcctgggc
     4921 cgccaccaga tttcgatcgc ctcggtcatc cagcacgagc ccgtgggcga cggcgcgggc
     4981 cgcaccgtgc cgctggtcat catgacctac caggccaagg aaggcgcagc ccaagccgcc
     5041 atggcagaga tcgaccggct gggctgcatg cgggagaaaa gcgtgcggct gcgagtactg
     5101 gattgatgat ttgggattta ggatttggga tttcaaggtt ccaaatccca aatccccaat
     5161 cctaaatccc aaatagagat gaagtacgca atcgtcatcc ccgacggctg tgccgatgag
     5221 ccgcaggctg cgctgggcgg aaagacgccg ctgcaagccg cgcggactcc ggcgatggat
     5281 gagatcgcgc ggttgggggt ggtggggcgc agcaataatg tgccggcgca tctgccgccg
     5341 ggttccgatg tcgccaactt gagcctcttc ggctacgacc cgaacaagta ctttacgggc
     5401 cgcgcgccgc tggaggccgc ggcgcagggg atccagctcg gtccggcgga ttgggcgatc
     5461 cgctgcaacc tggtaacgat cgaaaaccag acgatgcggt cgttcacggc cgatcacgtc
     5521 tcctcggccg aagcggcggc gctgctgaag acgtgccagg agaagctggg gtccgccgag
     5581 ttggaattca ttcccggcgt gagctaccgc aacctgctga tctatcgcgg ggcgaagcag
     5641 ccggcgccct ttacccgcga tacgcgggcc acaccgccgc acgacctgac cgacaagtcc
     5701 gtcgccgacg acttcccccg cggccccggc agcgacttcc tcaacgacct gatgggccgt
     5761 tcggtcgccc tgtttgccga gcatccggtg aatgccgctc gcaaggccgc cggcaagctg
     5821 ccggcgacga acgtctggct gtggggcctg ggcaaaacac ccgctttgac gccgtttttt
     5881 gagctgcaca aaaaacgcgg caagatgatc accgcggtgg atctgttgcg gggcctggcg
     5941 gcgctgatcg gctgggagcg gatcgaggtg cccggcgcga ccggctatct cgacaccgac
     6001 tatgccgcca agggccgcta cgcggtcgag gctctgacga ccaccgacat cgtctgcgtc
     6061 cacgtcgaag ccaccgacga agcttcgcac gagggccggg ccgatgccaa gatcaaggcc
     6121 ctggaagaga tcgacgcg
//
LOCUS       DASRJB010000630        10417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_469001, whole genome shotgun sequence.
ACCESSION   DASRJB010000630 DASRJB010000000
VERSION     DASRJB010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469001"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..3062
                     /gene="aceE"
                     /locus_tag="VFB80_16710"
     CDS             315..3062
                     /gene="aceE"
                     /locus_tag="VFB80_16710"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZN35473.1"
                     /translation="MSEVDANSAVDEAASAAAANGEGPHITPVPPVDIDPAETQEWLE
                     SLEYVLNTKGPERVKQLLAVLDRKARQEGVELPYALNTPYINTIHVSQQPRYPGNREL
                     ERRIKSIIRWNAMAMVHRANKHFDGLGGHISTFASSATLYEVGYNHFFRGRGEGGYEG
                     DLIYFQGHASPGMYARAFLEGRLSEDNLEHFRRELQTTRGLSSYPHPWLMPDFWEFPT
                     VSMGLGPIMAIYQARFNEYLKDRGIKDTSGRKVWAFLGDGECDEPETLGAITLAGREQ
                     LDNLIFVVNCNLQRLDGPVRGNGKIIQELEGLFIGAGWNVIKVVWGGDWDPLLEKDHS
                     GLLVKRMGEVVDGQYQKYTVMPGSYIREHFFGKHPELLALVENYSDEKLWKLRRGGHD
                     PEKVFAAYKAALEHKGQPTVILAKTIKGYGLGEAGEGRNTAHNEKKLNEEELLEFRTR
                     FGIPISDDEVAQAPFYRPPENSLEMKYLRERRADLGGFVPSRPTTHPRTETPRIDEYK
                     EFLAGSQGKEMSTTMAFVRLLRKLCRDKQVGKQVVPIVPDESRTFGMEGMFGEIGIYS
                     HVGQLYEPVDSHQIAKYKESVSGQILEEGITEAGSMSSFNAAGTAYSTHGINMIPFYI
                     YYSMFGFQRIGDLIWAAQDMRAKGFLVGGTAGRTTLNGEGLQHQDGHSLLNAIAFPSV
                     RAYDPAYAYEMAVIVLDGMRRLYEEGDTAIYYLMAGNENYVHPAMPSGVEEGIVRGMY
                     RLQTREVDGAKAKVNLFGSGAILRHVLLAQELLAAKFQIASNVWSVTSYTELRRDAHR
                     AERWNMLHPDKKPRQSYVEKTLAGEQGLFVAASDYVRALSEQIGRWVPGELFALGTDG
                     MGRSETRETLRRHFEVDAESIAIATLYRLHKQGVFEAKFVAQAIKDLGVNPEKIDPYF
                     A"
     gene            3164..4531
                     /locus_tag="VFB80_16715"
     CDS             3164..4531
                     /locus_tag="VFB80_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZN35474.1"
                     /translation="MPTEVKLPNLGDGIDTGDVLELLVKEGDTIAKDQGIVELETGKA
                     TLQVPSSAAGKILKVHIKPGQTIPVGATLLTLEGATADVAQAPPKTAPKPAKAPPPKP
                     APALPPAAEAPSPPRPAAAPTPVPPAPPAPASVQAPAPAPSPSPSPLPEPTTDGHVAA
                     GPAVRRFAREVGVDLARVTGSGPGGRITREDVLTVVRQASQTAAAAPTAKGKGSTATA
                     RDNWGPIRVEKMTKIRKVIAAKMHESWMTCPRVVNFDDADVTDLEKIRASSKDDYAKK
                     GIKLTSLPFVIKAVASALRNHPLLNASVDLEQGQIVFKEYVNIGIAVDTERGLVVPSL
                     RSADELSIADTARALAALAERVRDGSFNMADLQGSTFTISNLGAVGGTYSTPIINTPE
                     VAILLVGRSRQLPVVVDDQIAVRLMMPLSLAYDHRLVDGATAARFLNEVIDYLEAPSR
                     LLLAP"
     gene            4684..5037
                     /locus_tag="VFB80_16720"
     CDS             4684..5037
                     /locus_tag="VFB80_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35475.1"
                     /translation="MSEIPEIPAERREQAAMLIRLYCKRPFRDHGRRRTDGPYKGWYQ
                     HDWGHVVQRIYRLFFDQQLDIPSATIASKAVRSEFPSRNDWEKRVGTAQAQIRMLALI
                     EEGLAPFAPVKTPVA"
     gene            complement(5114..5890)
                     /locus_tag="VFB80_16725"
     CDS             complement(5114..5890)
                     /locus_tag="VFB80_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35476.1"
                     /translation="MGEFGICMGLCLLAIPAFFIGLVLLTVAVAMWERRVVWYFVPVD
                     TATGRPAALDPNNPYAASGVPLPQVPPPSVDASQANAAMKTMGFEFLGTCGDAKGAKY
                     HVRYDFWVAPERDVLVNVGCGKVIGIDVDARWLYTRLQDGRTIVTVSDQSASEYDLTG
                     QRVEALVSAISFQQLLSAHRKRIKAQFSLPLSFGPDPLKDLRTEIKQRVDTLVASGYA
                     KHLDPQQATWSYTLLGALLFAPMANGQALRRQLIPDSGQP"
     gene            complement(5910..6392)
                     /locus_tag="VFB80_16730"
     CDS             complement(5910..6392)
                     /locus_tag="VFB80_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HZN35477.1"
                     /translation="MSYRVRLEKEHHVFSAAHFITFAGDVCERLHGHNYRVAVEVEGP
                     LDENQYVIDFVALRDELKTILDDLDHRMLLPTGHPLIRVTEGASEVEVVFTPDGRRWV
                     FPHGDCAILPVANTTAELLARYIGERLRAAIDAQIGQTPARLIVAVDENHGQWGICEL
                     "
     gene            complement(6429..7289)
                     /locus_tag="VFB80_16735"
     CDS             complement(6429..7289)
                     /locus_tag="VFB80_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZN35478.1"
                     /translation="MKAAPPLSIGHCATLACLLEATAPKVGNVHRGADFDGLTFTDFA
                     VSAALIAPAMESAAQIGVGRAVLHAITATRSVVATNTNLGMALLIAPLAAVARSRRLT
                     TASVSEVLQSLTADDSRLVYEAIRLAQPGGLGQAESMDVADGAPPSLLAAMQAAAARD
                     QIAEQYATNFRLVLDRLLPGLITGRAKGWSLTEAIVHTQLASLAEFGDSLIARKCGQE
                     LSQQAAARAARALEAGSPGGEAYHAALADFDFWLRADGNRRNPGATADLIAAALFAGL
                     RDGLLPPPWN"
     gene            complement(7293..8153)
                     /locus_tag="VFB80_16740"
     CDS             complement(7293..8153)
                     /locus_tag="VFB80_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN35479.1"
                     /translation="MPWRDLYPFQSRYLQLGPHRLHYLDQGAGQPLLLVHGNPTWSFY
                     WRNLIRGLAGDFRCLAVDHIGCGLSDKPQVYPYTLPRRIDDLVQVVERLDLTGTTLLA
                     HDWGGAIGLGTVQRLPQRFARIVLFNTGAFPPPFVPWRITACRLPLAGTLAVRGLNAF
                     ARAALSMAVEKPERMTADVRAGLLAPYDSWASRVAVDRFVKDIPFSPRHPTWQILERI
                     EAGLDSLAHLPIQLIWGMRDWCFRPECLERFLKHWPSAEVHRFEDCGHYVVEDAHERI
                     VPLVRAFLKR"
     gene            complement(8306..9844)
                     /locus_tag="VFB80_16745"
     CDS             complement(8306..9844)
                     /locus_tag="VFB80_16745"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN35480.1"
                     /translation="MKSIWPIATVFAALFANFAAAAQLELKAVDKATGQPVAARVHLK
                     NAAGKPVKPPGKAPYWKDHFVFDGSVTLELPPGSYTFEMECGPEYKLGSGYFTLDRDS
                     NDTKTVEMVRFVEMKKEGWWSGELHIHRPPAEIELHLRAEDLHIGPVMTWWNNQNQWK
                     EKALPEQPLVKFDGDRFYHLMAGEDEREGGALLYFNLPQPLPIAGSKREYPSPLKFLD
                     QARQQAGVHVDVEKPFWWDMPLWVATGKVDSMGLANNHQHRDGMLDNEAWGKPRDPAR
                     FPSPHGNGRWSQELYYKLLNCGLRIPPSAGSASGVLANPVGYNRVYVHVDGELTWEKW
                     FENLRKGQVVVTNGPLLRPRVLGPGAPPPGALPGHVFTAEKGQTIELQMALQLSTRDK
                     IDYLEVIRDGQTVHEVRLDQWAKAGGKLPPVAFDKSGWLLVRAVTNNEKTYRFASSGP
                     YYVEIDGQRRVSKEAAQFFMDWVFERARRVKLDDADQQAEILATHKLARDYWQKMVNE
                     ANAE"
     gene            complement(9935..>10417)
                     /locus_tag="VFB80_16750"
     CDS             complement(9935..>10417)
                     /locus_tag="VFB80_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35481.1"
                     /translation="ATAEVSQGGWLATGDLGRLDADGFLYITGRKKEILVTSGGKNIA
                     PVFLESLLTQDPLILQAMVVGDGRSHLGALIIPNRDLVKAELARGTAADEQALIRARI
                     DACLANVSPYEQVRKFVLLPRPFSIEAGELTPKLSLRRQEITAHFAAEIESMYRAAAC
                     "
BASE COUNT         1942 a         3357 c         3353 g         1765 t
ORIGIN      
        1 caaatcagct cgcggaaaaa tgctctgcga atgcatacat atgcggatcg ccccggaaat
       61 ggggcgcttg cgtgcgacac gacgtgcgtc ataaactatt tcacttgaac aacttacaac
      121 tgaagtgacg ctttggttag caatccgcca gcccctttac ctacctaggc aaaaggcgga
      181 gaatgctgca cagagacgtt ttatgcacga taggcttagc ggcgtataaa ctaccactgc
      241 cacgagcttg gatggtttga cggaattaca tctcacctta gccgattatt tgctcggcgg
      301 attcggaatg ctcgatgtct gaagttgatg ccaattccgc ggtggacgag gccgcctcgg
      361 cggctgcggc caacggcgaa ggtccacaca tcacgccggt gcccccggta gacatcgatc
      421 cagccgagac ccaggaatgg ctcgagtcgc tcgaatatgt gctgaataca aaagggccgg
      481 agcgggtcaa gcagctcctg gccgtgctcg accgcaaggc ccggcaagag ggggtcgagc
      541 tgccctacgc cctcaatacg ccctacatca acacgatcca cgtcagccag cagccgcgat
      601 atccgggcaa ccgcgaactg gagcggcgga tcaaaagcat cattcgctgg aatgcgatgg
      661 cgatggtcca ccgggccaac aagcacttcg acggcttagg cggacacatt tcgacgttcg
      721 cttcgtccgc cacactgtat gaagtcggct acaaccactt cttccgcggc cgaggcgagg
      781 gcggctacga aggcgacctg atctacttcc aggggcatgc gtcgccggga atgtacgcgc
      841 gggcctttct cgaaggccgg ctgagcgaag acaacctgga gcattttcgc cgcgagctgc
      901 aaacgacacg gggcctgtcg agctatccgc atccctggct gatgccggat ttctgggaat
      961 tcccgacggt ctcgatgggc ctggggccga tcatggccat ttaccaggcg cggttcaacg
     1021 aatacctcaa ggaccgcggc atcaaggaca ccagcggccg caaggtctgg gcgtttctgg
     1081 gcgatggcga gtgcgacgag cccgaaacgc tgggcgcgat cacgctggcc ggccgggagc
     1141 agctcgacaa cttgatcttc gtcgtcaact gcaacctcca gcggctggac ggcccggtcc
     1201 gcggcaacgg caaaatcatt caagagctgg aagggctgtt catcggcgcg ggctggaacg
     1261 tgatcaaagt cgtttggggc ggcgactggg atccgctgct ggagaaggac catagcggcc
     1321 tgctcgtcaa acggatggga gaggttgtcg atgggcagta ccagaagtac acggtgatgc
     1381 ccggctcgta tattcgcgag cactttttcg gcaaacatcc cgagctgctc gcgctcgtcg
     1441 agaactattc ggacgagaag ctctggaagc tgcgccgcgg cggccatgac ccggagaagg
     1501 tctttgccgc gtacaaggcg gcgctggagc acaagggcca gccgacagtc atcctggcga
     1561 agacgatcaa gggctatggc ctgggcgaag cgggcgaggg ccgcaacacg gcccacaacg
     1621 aaaagaagct caacgaagag gaactgctgg agttccgcac ccggttcggc attccgattt
     1681 ccgacgacga agtggcccag gccccgttct atcggccgcc cgaaaacagc ctggagatga
     1741 aatatctccg cgagcggcgg gccgacctgg gcgggttcgt tcccagccgg ccgaccacgc
     1801 acccgcggac cgagacgccg cggatcgacg agtacaagga attcctcgcc ggcagccagg
     1861 gcaaggagat gtccacgacg atggcgttcg tgcggctgct gcgaaagctc tgccgcgaca
     1921 agcaggtggg caagcaggtc gtgccgatcg tgcccgacga atcgcggacc tttggcatgg
     1981 aaggcatgtt cggcgagatc ggcatctact cgcacgtcgg ccagctctat gagccggtcg
     2041 attcccacca gatcgccaag tacaaggaat cggtcagcgg ccaaatcctg gaagagggga
     2101 tcaccgaagc gggctcgatg agcagcttca acgccgccgg caccgcgtac agcacgcacg
     2161 ggatcaacat gatcccgttc tacatctact attcgatgtt cggcttccag cggatcggcg
     2221 atctgatctg ggcggcccag gacatgcggg ccaaaggctt tctcgtcggc ggaacggcgg
     2281 gacgcacgac gctcaacggc gagggcctgc agcaccagga cgggcacagc ctgctgaatg
     2341 caatcgcctt ccccagcgtg cgggcctacg acccggcgta tgcctatgaa atggcggtga
     2401 tcgttctcga cggcatgcgg cggctgtacg aagaaggcga cacggccatc tattacctga
     2461 tggcgggcaa cgagaactac gtccacccgg cgatgcccag cggcgtcgaa gaggggatcg
     2521 tccgcgggat gtaccggctg caaacgcgcg aggtggacgg ggcgaaggcc aaggtgaatc
     2581 tcttcggcag cggcgcgatc ttgaggcatg tgcttctcgc gcaggagctg ctggctgcga
     2641 agttccagat tgcctcgaac gtctggagcg tcacgagcta taccgagctg cgccgcgacg
     2701 cccaccgcgc cgagcgctgg aacatgctgc atccggacaa aaagccgcgg cagagttacg
     2761 tcgaaaaaac gctggccggc gagcagggcc tgttcgttgc cgcgtcggac tacgtgcgtg
     2821 cgctgagcga acaaatcggc cgctgggtgc ccggcgagct ctttgccctg ggcaccgacg
     2881 gtatgggccg cagcgaaacc cgcgaaacgt tgcggcggca tttcgaggtc gatgccgaat
     2941 cgatcgccat cgccacgctc taccggctgc acaaacaggg cgtgttcgag gcgaagtttg
     3001 tggcccaagc gatcaaggac ctcggtgtaa acccggaaaa gatcgatccc tactttgcat
     3061 gagatttcag atttaggatt tcagatttga ggagggattc ccctaactga cttgctccaa
     3121 atctgaaatc tggcatctgc aatctgaagt cctaaactga tccatgccca ccgaagtcaa
     3181 actccctaat ctcggcgacg gcatcgatac gggcgacgtg ctggagctgc tcgtcaaaga
     3241 gggcgacacg atcgccaagg accagggcat cgtcgaattg gaaacgggga aggccacgct
     3301 gcaggtgccc agctcggcgg cgggcaagat tctcaaggtg cacatcaagc cgggacagac
     3361 gattcccgtc ggcgcgacgc tgctgacgct cgaaggcgcg accgccgatg tcgcgcaggc
     3421 cccgccgaag accgcgccca agcccgccaa agcgccaccg ccgaaaccgg ctcccgcgct
     3481 gccaccggcc gcggaagcgc cgtctccgcc gaggccagcc gctgcgccca cgccagttcc
     3541 gcctgcgcct cctgcgccgg cttccgtgca ggccccagcc ccagccccca gccccagccc
     3601 tagccctctg ccagagccga ccacggacgg ccacgtcgcc gccggtccgg ccgtccgccg
     3661 ctttgcccgc gaggtcggcg tcgatctggc tcgcgtcacc gggtctgggc ccggcggcag
     3721 gatcacgcgc gaggatgtgc tcaccgtcgt gcggcaagcc agccagacag ccgccgcggc
     3781 gccaaccgcc aagggaaagg gctcgaccgc aacggcccgc gacaactggg ggccgattcg
     3841 cgtcgagaag atgaccaaga ttcgcaaggt catcgcggcc aagatgcacg agtcgtggat
     3901 gacctgcccg cgggtggtca atttcgacga cgccgacgtg accgacctgg agaagatccg
     3961 cgccagcagc aaggacgatt acgccaagaa ggggatcaag ctgacgagcc tgccgttcgt
     4021 gatcaaggcg gtggccagcg ccctgcggaa tcatccgctg ctcaacgcgt cggtcgatct
     4081 ggaacaggga cagatcgtct tcaaggaata cgtcaacatc ggcatcgcgg tcgataccga
     4141 gcggggcctg gtcgtgccca gcctgcgcag cgccgacgaa ctgagcatcg ccgacaccgc
     4201 ccgggccttg gcggctctgg ccgagcgcgt tcgcgacggc agtttcaaca tggccgattt
     4261 gcagggctcg accttcacga tcagcaacct gggcgcggtc ggcggcacct acagcacgcc
     4321 catcatcaac acgcccgaag tggcgatcct gctcgtcggc cgctcgcggc agctgccggt
     4381 cgtcgtcgat gaccagatcg ccgtgcgcct gatgatgccc ctctcgctgg cctacgacca
     4441 ccgcctcgtc gatggcgcga cggccgctcg cttcctgaac gaagtgatcg actacctcga
     4501 agcccccagc cggttgttgc ttgctccgta gatcaagcgt ccgcgcttgc tcgcttccac
     4561 ccgggcaatt ccgcacaagc gagggcgttt tgccgcgagc agtgggcggc gccttcgagt
     4621 acaatgctag ttacctggtg tctctccgag ccagtgtcca tcccactcgc ctggagacca
     4681 gcgatgtcag agattcccga aattcccgca gaacgccgcg agcaagcggc catgctgatc
     4741 cggctgtatt gcaaacggcc gtttcgcgat catggccgcc ggcggacgga cggcccgtat
     4801 aagggctggt atcagcacga ttggggccac gtcgtgcagc ggatctatcg cctgtttttc
     4861 gaccagcaac tcgacattcc cagcgcgacg attgcctcca aggccgtccg cagtgaattc
     4921 cccagccgca acgactggga gaagcgcgtc ggcacggctc aggcccagat ccgcatgctg
     4981 gcgctgattg aagagggcct ggcgccgttt gcgccggtca agactccggt cgcctgactg
     5041 cggcgtgatg tatttctggg ggctggctgc gctgtgaccc tggaggcggg ctgcgcgtgg
     5101 cgggttgtgc cgatcaaggc tggccgctgt cggggatcag ctgccgccgc agcgcctggc
     5161 cgttggccat gggagcaaac agcagcgcgc ccagcagcgt atagctccaa gtcgcctgct
     5221 gcggatcgag gtgcttggcg tagccgctgg cgacgagcgt atcgactcgc tgcttgattt
     5281 cggtccgcaa gtccttgagc ggatctgggc cgaacgacag cggcagcgag aactgcgcct
     5341 tgatccgctt gcggtgggcg ctcaagagtt gctggaacga gatggccgaa acgagcgcct
     5401 cgacgcgctg gccggtgagg tcgtattcgc tggcgctctg atcgctgacg gtgacgatcg
     5461 ttcgcccgtc ctgcaggcgg gtgtagagcc agcgggcatc cacgtcgatg ccgatcacct
     5521 tgccgcagcc gacgttgacc agcacgtcgc gctcgggcgc gacccagaag tcgtaccgca
     5581 cgtggtactt cgcccccttg gcgtcgccgc acgtgcccag gaattcaaaa cccatcgtct
     5641 tcatcgcggc gttggcctgc gacgcgtcca ccgagggcgg cggcacctgc ggcagcggca
     5701 cgcccgacgc ggcgtacggg ttgttgggat cgagcgcagc gggcctgccc gtcgccgtat
     5761 caaccggcac gaaataccag acgacacgcc gctcccacat cgccaccgcg accgtgagca
     5821 gcaccagacc gatgaagaac gcggggattg ccagcaggca gagccccatg cagatgccga
     5881 attcacccat ggatgtcgtg cccgcgattc tacaattcgc aaattcccca ctgaccgtgg
     5941 ttctcgtcca cggcgacgat cagccgggcc ggagtctggc cgatctgggc gtcgatcgcc
     6001 gcccgcagcc gctcgccaat atagcgggcc agcagctcag ccgtggtgtt ggccacgggc
     6061 aagatggcgc agtcgccgtg gggaaagacc cagcggcggc cgtcgggcgt gaagacgacc
     6121 tccacttcgc tagcgccttc ggtgacgcgg atcagcggat ggccggtcgg cagcagcatc
     6181 cggtggtcga ggtcgtcgag gatcgtcttc agttcgtcgc ggagggcgac gaagtcgatc
     6241 acgtactggt tctcgtcgag cgggccttcc acttcgaccg ccacgcggta gttgtgcccg
     6301 tgcagccgct cgcagacgtc gcccgcgaac gtgatgaagt gggcggcgct gaagacgtga
     6361 tgttctttct cgaggcggac gcggtaggac atagtggcca gtcggtcggt tggtcggtga
     6421 tcggtcggtc agttccaagg cggtggcagc agtccgtcgc ggaggcccgc gaacagggcc
     6481 gcagcaatca ggtcggccgt tgcgccgggg ttgcggcggt tgccgtcggc ccgcagccag
     6541 aagtcgaagt cggccagagc ggcgtgatag gcttcgccgc ccggcgatcc cgcttccaga
     6601 gcccgcgcgg cccgcgccgc ggcctgctgc gacagctcct ggccgcactt gcgggcgatc
     6661 aggctgtcgc cgaactcagc cagcgacgcc agttgcgtgt ggacgatcgc ctcggtgagc
     6721 gaccagccct tggcccggcc tgtgatgagc ccgggcagca gccggtcgag cacgaggcgg
     6781 aagttcgtcg cgtattgctc ggcgatctgg tcgcgggcgg cggcggcctg catggccgcc
     6841 agcaggctcg gcggcgcgcc atccgccacg tccatcgact cggcctgccc caggccgccg
     6901 ggctgcgcca ggcggatcgc ctcgtaaacc agccggctat cgtcggccgt cagcgattgc
     6961 aggacctcac tgacgctggc cgtagtcagc cgccggctgc gggccacggc cgccagcggt
     7021 gcgatgagga gcgccattcc caggttggta ttcgtcgcga cgaccgaccg tgtcgccgtt
     7081 atcgcatgga ggaccgcgcg gccaacgccg atttgggcag ccgattccat cgccggcgcg
     7141 atcagcgcgg cgctgaccgc gaaatcggtg aacgtgaggc cgtcgaagtc ggccccgcgg
     7201 tgtacgttgc cgaccttcgg cgccgtcgct tccagcagac aggccagcgt ggcacagtgg
     7261 ccgatcgaaa gtggtggtgc agctttcatc gttcaacgtt tcagaaacgc tcgcaccagc
     7321 ggcacgatcc gctcgtgtgc gtcttcgacg acgtagtggc cgcagtcttc gaaccggtgg
     7381 acctcggcgc tgggccagtg cttcagaaac cgctccaggc actcgggccg aaagcaccag
     7441 tcgcgcatgc cccagatcaa ttgaatcggc aaatgcgcca ggctgtccag ccccgcctcg
     7501 atccgctcca ggatctgcca ggtcgggtgc cgcggagaga acggaatgtc cttcacgaag
     7561 cgatcgactg caacgcggct ggcccagctg tcgtacggcg cgagcaagcc cgcccggaca
     7621 tcggccgtca tccgctcggg cttctcgact gccatcgaca gggccgcgcg ggcgaaggcg
     7681 ttcaggccgc ggacggccag cgtgcccgcc aggggcaagc ggcaggcggt gatccgccag
     7741 ggtacaaacg gcggcggaaa tgcgccggta ttgaacagca cgatccgggc aaagcgctgg
     7801 ggcagtcgct gcacagtgcc caggccgatc gccccgcccc agtcgtgcgc cagcagcgtc
     7861 gtacccgtca gatcgagccg ctcgacaacc tgcacgaggt cgtcgatccg ccgcgggagc
     7921 gtgtagggat acacttgcgg cttgtcggac agtccgcagc cgatgtgatc gacggcgagg
     7981 cagcggaaat cgcccgccag gccgcgaatc agatttcgcc agtagaacga ccaggtcgga
     8041 ttgccgtgaa ccaacaagag cggctgcccg gcgccttggt cgagatagtg cagccggtgc
     8101 gggccgagct ggagatagcg cgactgaaaa ggatagaggt cgcgccaggg catttcggat
     8161 ttcggatgtc agttttcaga ctgcagtttt gtcgtttaac ccggagctgg aggcgacggc
     8221 gtctccccga attcgcctgc gccttcggct gcgggttaaa cgaggcaacc aatcataaat
     8281 ctaaaatccc aaatcataaa ttcccctact ccgcgttcgc ctcgttcacc attttctgcc
     8341 aatagtcgcg agcgagctta tgcgtagcga gaatctccgc ctgctgatcg gcgtcgtcga
     8401 gcttgacgcg gcgggcccgc tcgaaaaccc agtccatgaa gaactgggcg gcttctttgc
     8461 tcacgcgacg ctggccgtca atctcgacat aatacggccc actgctggca aagcggtagg
     8521 ttttctcgtt gttggtcaca gcgcggacca gcagccagcc gctcttgtcg aacgcaacgg
     8581 gcggcagctt gccgccggcc ttggcccact ggtccagccg gacttcgtgg acggtctggc
     8641 cgtcccggat gacctcgaga tagtcgatct tgtcgcgcgt cgaaagctgc aatgccatct
     8701 gcagctcgat cgtttgtccc ttttcagccg taaagacgtg ccccggcaaa gcgcctggcg
     8761 gcggtgcgcc ggggccgagc acgcgcggcc gcagcagcgg gccgttggtg acaacgacct
     8821 gccccttgcg gagattctcg aaccacttct cccacgtcag ctcgccgtcc acgtgcacat
     8881 agactcggtt gtagcccacc ggattggcca agacgcccga ggcgctgccg gccgagggcg
     8941 gaatccgcag cccgcagttg agcagcttgt agtagagctc ctgggaccag cggccgttgc
     9001 cgtgtggcga gggaaaacgc gccggatcgc gcggcttgcc ccaggcttcg ttgtcgagca
     9061 tcccgtcgcg gtgctggtgg ttgttggcca ggcccatcga atcgaccttg cccgtcgcca
     9121 cccacagcgg catgtcccac cagaagggct tctccacatc gacgtgcacg cccgcctgct
     9181 gccgggcctg atccaggaat ttcagcggcg aaggatattc ccgcttcgac ccggcgatcg
     9241 gcaagggctg cggcaggttg aagtacagca gtgcgccgcc ttcgcgctcg tcctcgccgg
     9301 ccatcaggtg atagaagcgg tcgccgtcga acttcaccag cggctgctcg ggcagcgcct
     9361 tttccttcca ctggttctgg ttattccacc aggtcatcac cgggccgatg tgcaaatcct
     9421 cggcccgcag gtgcagctca atttccgccg gcgggcgatg gatgtgcagt tcgcccgacc
     9481 accaaccctc cttcttcatt tcaacgaacc gcaccatctc gaccgtcttg gtgtcattgc
     9541 tgtcgcgatc cagcgtgaaa tagccgctac cgagcttgta ctccggaccg cactccattt
     9601 cgaacgtgta gctgccggga ggcagctcaa gggttaccga cccatcaaaa acaaagtggt
     9661 ctttccagta cggcgccttg cccggcggct tcaccggctt gccggcggca ttcttcaagt
     9721 gcacgcgggc ggccaccggc tggccggtag ccttgtccac ggccttcaat tccagctggg
     9781 ccgcggcggc aaagttggcg aacaacgccg cgaagactgt cgcaatcggc cagatcgatt
     9841 tcatgcgatg tgctccgtag ccgaattcgc cagaattcga acccgcagcg gacagaccga
     9901 attctggcga attgggctac acggtagcgc aagtttagca cgccgcggca cggtacatcg
     9961 attcgatttc ggccgcgaag tgggccgtaa tctcttgacg gcggaggctg agcttgggcg
    10021 tcagctcgcc ggcttcgatc gaaaacggcc gcgggagcag gacgaacttg cggacctgtt
    10081 cgtagggcga aacgttcgcc aggcaggcgt cgatccgtgc gcgaatcagc gcttgctcgt
    10141 cggccgccgt accgcgggcc agttccgcct tcacgaggtc gcgattcggg ataatcagcg
    10201 cccccaggtg cgaccgcccg tcgccgacga ccatcgcctg caggatcagc gggtcctgcg
    10261 tcagcagcga ttcgaggaac accggcgcga tgttctttcc gccggacgtg acgagaattt
    10321 ccttcttgcg gccggtgatg tagagaaagc cgtcggcatc gagccggccc aggtcgccgg
    10381 tggcgagcca gccaccctgc gagacttcgg cggtcgc
//
LOCUS       DASRJB010000631         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_469081, whole genome shotgun sequence.
ACCESSION   DASRJB010000631 DASRJB010000000
VERSION     DASRJB010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469081"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFB80_16755"
     CDS             complement(<1..1027)
                     /locus_tag="VFB80_16755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35482.1"
                     /translation="MKAEEIFLAVLERTSPTKRAAYLDEACAGNAALRALVEGLLYSH
                     HEAGSFLDAPLFDAAATMNQPHAERTGAQIGPYKLLQQIGEGGFGVVFMAEQLEPVRR
                     KVALKLIKPGMDTRQVIARFEAERQALAVMDHPHIAKVLDAGATESGRPYFVMELVRG
                     VPITHYCDENHLSVRERLGLFASVCQAIQHAHTKGIIHRDIKPTNVLVTRQEGQPVVK
                     VIDFGIAKALGQQLTDKTLFTEFAQMIGTPLYMSPEQAELSSNDIDTRSDIYSLGVLL
                     YELLTGSTPVSKEQLKAAAFDEIRRIICEDEPQKPSTRISTADAAPSIAAHRHTEPAK
                     LASLVRGE"
     gene            complement(1109..1507)
                     /locus_tag="VFB80_16760"
     CDS             complement(1109..1507)
                     /locus_tag="VFB80_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN35483.1"
                     /translation="MSDVTRILAQIDSGDPAAAEKLLPLVYDELRKLAAAKLAHEQPG
                     QTLQATALVHEAYLRLVRPGVGENRLLPTPDFRPPTPGFRYFGGLTIEQAAEVLKTSR
                     VTAHRYWTVARGWLHQEITGEQRTPAESAG"
     gene            complement(1549..2583)
                     /locus_tag="VFB80_16765"
     CDS             complement(1549..2583)
                     /locus_tag="VFB80_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35484.1"
                     /translation="MLMPDRLMLAVCCLFLAQTGAAAEPLPAGVVCLLEDNAAELLPL
                     LSNPGGDLGEGEVEHKVVFSGRSAVKITSYQKYFNLLPGWAHRIAEKPAAGEYRYLRF
                     AWKSAGLTGIMLQLHDARDWHIRYVAGANKFGWAANQVAESPPPEWTLVTLDLFKDFG
                     EREIHGIALTAFDGAAGYFDHIYLGRTIDDLDAIDATGRADAGPIQLTPEEVDKHLEQ
                     LRSADGSIAYRAFWTLAAGDGSVRALLAGRTSAEPAAKAEPAAIEQWLRDLNADEFDV
                     REKATMQLAAHLAAYRERLEEELRRTASAESRARLAGILAAARKPLTDYERTEQQIQR
                     ILQTIKRRQG"
     gene            complement(2995..>3346)
                     /locus_tag="VFB80_16770"
     CDS             complement(2995..>3346)
                     /locus_tag="VFB80_16770"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN35485.1"
                     /translation="LWRCDLQPGSWLYIPSGYWHRAEARELSISLAIGLAPHCAMDVW
                     DFLRRELLQSLEWRQRLPILGRASPLAPDELRARVAQVFQALGSDLAHRLADPGAVDR
                     YLRSRGGERTAADL"
BASE COUNT          576 a         1078 c         1082 g          610 t
ORIGIN      
        1 gctcgccgcg caccaggctg gcgagcttcg ctggctccgt gtgacgatgg gcggcaatgc
       61 tgggggcggc atcggcggtg ctgatccgcg tgctgggctt ctgcggctca tcctcgcaga
      121 taatgcggcg gatttcgtcg aaggcggccg ctttgagctg ctccttgctg actggcgtcg
      181 agccggtcaa taactcgtag agcagcacgc cgagcgagta gatgtcgctg cgggtgtcga
      241 tgtcgttgct ggagagctcc gcctgctcgg ggctcatgta gagcggcgtg ccgatcatct
      301 gggcgaattc ggtgaacagc gtcttgtcgg tgagctgctg ccccagcgct ttggcgatgc
      361 cgaagtcgat cactttcacc accggctgcc cttcctgccg cgtgaccagg acgttagtcg
      421 gcttgatgtc gcggtggatg attcctttgg tatgggcgtg ctggatcgcc tggcagacgg
      481 aagcaaacag tcccagccgc tcgcggacgg acaaatggtt ctcgtcgcag tagtgcgtga
      541 ttggaacgcc gcgaacgagc tccatgacga agtaggggcg gccggactcc gtggctcctg
      601 cgtcgagcac cttggcgatg tgcgggtggt ccatcacggc cagcgcctgc cgctccgcct
      661 cgaaacgggc aatcacttgc cgcgtgtcca tccctggctt gatcagcttg agcgccacct
      721 tgcggcggac cggttccagc tgttcggcca taaagacaac gccgaaaccc ccttcgccga
      781 tctgctgcag cagcttgtag gggccgatct gcgcaccggt ccgctcggcg tgcggttgat
      841 tcatcgtcgc ggctgcgtcg aacagcggtg cgtcgaggaa gctgccagcc tcgtggtgcg
      901 aatacagcag gccttccacc agggcccgca gggcggcatt gcccgcacag gcctcatcca
      961 ggtacgccgc gcgcttggtg ggcgacgttc gctccagcac cgccaggaag atttcttcgg
     1021 ccttcatgga accgcactcc ctgagcaaac ggctgtgctc atcatccaat gcgacgccgg
     1081 cggcccgcgc gctccagccg ccggctcatt atccggcgga ttccgccggc gtgcgctgtt
     1141 cgccggtgat ctcctggtgc aaccaacccc gggcaacggt ccagtaccgg tgagccgtaa
     1201 cccgtgaagt cttcagcact tccgccgcct gttcgatggt cagtccgccg aagtagcgga
     1261 agccaggggt cgggggtcgg aagtcggggg tcggcagcag ccgattctct ccgactcccg
     1321 gcctcactag ccgcaaatac gcctcgtgca ccagcgccgt tgcctgcagc gtctgtcccg
     1381 gctgctcatg ggccagtttc gccgcggcca gcttccgcag ctcgtcatag accagcggca
     1441 gaagcttctc ggcggcggcc ggatcgccgg agtcgatctg cgccaaaatg cgcgtcacgt
     1501 cggacataac gcaattctgc tgtgccgacc agcgagccgc aattgtgtct agccttgccg
     1561 ccgcttgatc gtctgcaaga tgcgttgaat ctgttgctcg gtcctttcgt aatctgtcag
     1621 cggcttgcga gcagcggcca gaatgccggc caggcgggcg cgtgattccg cggaagccgt
     1681 gcggcgtaac tcctcctcga ggcgttctcg gtaagccgcc agatgggccg ccagctgcat
     1741 cgtggccttc tcgcgcacgt cgaactcatc ggcgttgaga tctctcagcc actgctcgat
     1801 cgcggcgggc tccgccttgg cagcaggttc ggccgatgtc ctgccggcga gcaaggcgcg
     1861 gaccgaacca tcgccggcgg ccaatgtcca gaacgcacgg taagcgatcg agccatcggc
     1921 gctccgcagc tgctccaagt gcttgtccac ttcttcgggc gtcaattgga tgggaccggc
     1981 gtcggcgcgg ccggtcgcat cgatggcgtc cagatcgtcg atcgtcctgc cgagatagat
     2041 gtgatcgaaa tacccggccg cgccatcgaa ggcagtcagg gcgatcccgt ggatctcccg
     2101 ctcgccgaaa tccttgaaca ggtccagggt caccagcgtc cattccggcg gcggcgactc
     2161 ggcaacctgg ttggcagccc agccgaattt gtttgcgccg gccacgtagc gaatgtgcca
     2221 atccctggcg tcgtgcagtt gcagcatgat tcccgtcagc ccggcggact tccaggcaaa
     2281 tcgcagatag cgatattcgc ccgccgcggg cttctcggcg attcggtggg cccagccggg
     2341 cagcaagttg aagtacttct gatagctcgt gatcttgacg gcgctgcgcc cggaaaacac
     2401 caccttgtgt tcgacttcgc cttcgcctaa gtcgccgccg gggttcgata gcagcggtaa
     2461 aagctcggca gcgttgtctt ccagcaggca cacgactccc gccggcagag gctccgcggc
     2521 ggcgccggtt tgcgccagga acaggcagca gacggcaagc atcaggcggt caggcatgag
     2581 catgtgaggc ctggtgcatc gtatccgggg agcgagtgac gccgcgtacc aggaatgtaa
     2641 cggcgctggt tcggaaatcg attttctgcc ctaaacgctg gcttgcgaat ataacgaaaa
     2701 ctgcctggag cgaaaaactc gatcgcgacc ccgtacgccg ctgatcgcgc cgcaagtccc
     2761 tgacaggtga cgacttccgg cgtagggccg ggcggttctc aggcattgcg aacataagcc
     2821 tttgagggcc cggcggggag ttttcgttat atttcgcggg cgacagcagc ggaggaaact
     2881 ccgccgcaga ttccacacac cgaattgcca gagagcttgt ccgcaggcgg cggagagcca
     2941 aaatggcagc cgcccgcgcc aaagggaact atactgcaaa tagcgtacca atgttcacaa
     3001 atcggccgcg gtccgctcgc cgccgcgcga ccgcaggtat ctgtctacgg cgccgggatc
     3061 ggcgagtcgg tgggcaagat cgctgcccag cgcctgaaac acctgcgcga ccctggcccg
     3121 cagttcgtcc ggggcaagtg gcgacgcgcg gcccaggatc ggcagcctct gcctccactc
     3181 gagcgactgc agcaactcgc ggcggagaaa atcccacacg tccatggcgc agtgcggcgc
     3241 gaggccgatg gccagcgaaa tcgacagctc gcgggcctcg gcccggtgcc aatagcccga
     3301 cggaatgtag agccagctgc cgggctggag atcgcagcgc cacagc
//
LOCUS       DASRJB010000632         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1873184, whole genome shotgun sequence.
ACCESSION   DASRJB010000632 DASRJB010000000
VERSION     DASRJB010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873184"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2788
                     /locus_tag="VFB80_16775"
     CDS             <1..2788
                     /locus_tag="VFB80_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN35486.1"
                     /translation="VARAVHHAHECGVIHRDLKPSNIMLDAAGRTHVMDFGLARREAG
                     EVTMTVDGKVLGTAAYMSPEQARGEGHQVDRRTDVYSLGVILFELLTGERPFRGNVRM
                     LLHQVLHDEPPSPRKLSSRVPRDLETICLKCLEKGPAGRYATALAFAEDLQRFDEGRP
                     VTARPLPSSVKLIRWCRRNPARAALAATIVLSLLALSIGGTWFSLRLTASDQARQQAL
                     VGQRLSERALAAEAESRRVQQLAALSGAAAARNVRRNVGWLQANLDDLARAASLARSE
                     EDRLRLRGLFVDCWAGHDLILVASHPQTVELSCLAYSPTGSYLAAAYNRPTFLTLNVA
                     IPIEFIPLAADQPGFRLSILNQVAIESEGLRHESIRSVAFSEDGDRFAAGTLKGFIHF
                     WDVRERKLIGSWRAHGDLVSSVVFEPGGATLLSCSRDGAIKRWNVDTRACLKTVSPGG
                     SVAALRRIAEGYLAVADAPVVLSPASLEIVRTITAWPGVSQVLAPHPDGHGLAIAPHE
                     ILIASGRNLEPIRKMSDSAPPRNDWVPQIDIDPSGRWLVAAQGAVLRLWDLPSGQLLL
                     EKSLDDRLLDVAFHPREPELALIVGDRVQRWRIVQSPLWRTIAAGSVPLASIAVRDEG
                     RWLTTVATAETSEPERSLLVTREALTGKELFRRTGDEGRTFGEVAQDPRRPLVMASYS
                     DGAQLAVFDVASGQRTALAEGASITSPRFGPDGSIWYACEAPPAFLRPASAQIVQAEL
                     PDLTPRPFWMNRFAQFSHRISAVMALVVDQDAIAFATGDKRLHVFHRDTRQEVASLPL
                     ISTARALTLDSGGKWLLCGDDAGMLTVFRFPSLERASAHAVHDAALTAIATAENGLLA
                     TGGRDRTLRLWRFAAGAVEPVLATGPLPQSPAQMHMTADGQYLALLLENDAAVRWIDL
                     AGLQAELSRHGLGW"
     gene            complement(2790..>4249)
                     /locus_tag="VFB80_16780"
     CDS             complement(2790..>4249)
                     /locus_tag="VFB80_16780"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZN35487.1"
                     /translation="QQKMYGPALYQAFCELKQTFDPGGLFNPGKIVEAQPLTANLRFG
                     PRYQTRPVETAFDFSDFGGLAQAAEQCGGVGACRKKLSGTMCPSYMATRDENDSTRGR
                     ANVLRLAISGQLGPIGLTDPALYPVLDLCLECKACKSECPTGVDLARMKSEFLHQYRL
                     GHGTKWRSRLLANVEQLAIWGHRFAPLSNWLAASRPVRWLAEAWFDLDRRRLPPVFAQ
                     QTFLEWWQMQTALAGRNVPDDRRLALFADTFTNFHEPAIPMAVVELATAAGWQVTVPP
                     RVCCGRPLISKGFLDDARRQAEKTVHALLPLALRGLPIVFCEPGCYSAVRDDHPHLLR
                     GEQQRDARLVSQACLTFEEWALQAGDALPLQAGPPKVLLHAHCHQKALVGTGPAQRLL
                     MRIPGCEVVDLDAGCCGMAGSFGYEREHYELSQAVGERRLFPAVREAASGTTIIASGF
                     SCRHQLAHFAATAAVHPAIALNALLNNSADTCKGG"
BASE COUNT          683 a         1416 c         1434 g          716 t
ORIGIN      
        1 ggtcgcccgg gccgtgcatc acgcccacga gtgcggcgtg atccaccgcg acttgaaacc
       61 gtcgaacatc atgctcgacg ccgcgggccg gacccacgtc atggattttg gtctggcccg
      121 gcgggaagcc ggcgaagtca cgatgactgt cgatggcaaa gtgctcggca cggccgctta
      181 catgtcgccc gagcaggccc gcggcgaagg gcatcaggtg gaccgccgca ccgatgtcta
      241 ttcgctgggt gtcatcctgt tcgaactcct gactggcgaa cggcccttcc gcggcaacgt
      301 ccggatgctg ctgcaccagg tcctgcacga tgagccgccc agtccccgta agttgagcag
      361 ccgcgtgccg cgcgacctgg agacgatctg cctgaagtgt ctggagaagg ggccggcagg
      421 tcgttatgcg accgcgctgg ctttcgccga ggacttgcag cggttcgacg aaggccgccc
      481 ggtgacggcc cggccgctgc cgagttccgt caagctcatc cgctggtgcc gtcgcaaccc
      541 ggcccgggcc gcgctggccg ccacgatcgt gctctcgctg ctggcgctca gcatcggtgg
      601 aacgtggttc agcctgcggc tgacggcgag cgaccaggcc cggcagcagg ctcttgttgg
      661 ccagcggctg agcgagcggg ccctggccgc cgaggcggag agccgccgcg tgcaacaatt
      721 ggcggcgctc tcgggcgccg cggccgcgcg gaacgtgcgc cgcaatgtcg gctggctcca
      781 ggcgaacctc gacgacctgg cccgcgcggc gagcctggcg cggtcggaag aggatcggct
      841 gcggctgcgg ggcctgtttg tcgattgctg ggccggccac gacctgattc tcgttgcctc
      901 gcacccccag acagtcgagc tgtcgtgcct ggcctacagc cccacgggct catacctggc
      961 ggcagcctat aaccggccca ccttcctgac gctgaacgtc gcaattccca tcgagttcat
     1021 tccgctcgcg gccgatcaac ccgggtttcg gctgtcgatt ctgaaccagg tggccatcga
     1081 atccgaaggt ctgcgtcacg agagcatccg gtccgtcgcg ttcagtgagg acggcgaccg
     1141 gttcgccgcc ggcacgctga aggggttcat ccatttctgg gacgtgcgcg agcgcaagct
     1201 gatcggatcg tggagggcgc acggcgacct cgtgagcagc gtcgttttcg aacccggcgg
     1261 cgcgacgctg cttagctgtt cgcgcgacgg cgcgatcaaa cgctggaacg tggacacgcg
     1321 tgcctgcctg aagacggtca gcccaggcgg atcagtcgcg gcgctgcgcc ggatcgcgga
     1381 gggatacctg gcggtggccg acgcgcccgt ggtgttgtca ccggcctcgc tggaaattgt
     1441 ccgcacgatc acggcctggc cgggcgtttc gcaggtgcta gcgccgcatc ccgatggaca
     1501 cggcctggcc atcgccccgc atgagatcct gattgcctcc ggccggaacc tggagcccat
     1561 ccgcaagatg tccgactccg caccgccgcg caacgactgg gtgccccaga tagacatcga
     1621 tccgtcaggc cgatggcttg tcgccgctca gggcgccgtg ctgagactgt gggacttgcc
     1681 gtcagggcag ttgctgctag agaagtcgct ggacgaccgg ctgctggatg tggcgtttca
     1741 cccccgcgag ccggagctgg ccctcattgt gggagaccgg gtgcagcggt ggcggatcgt
     1801 gcagtcgccg ctgtggcgga cgattgcggc cggatcggtt ccgctggcca gcatcgctgt
     1861 gcgcgacgag ggccgctggc tgaccaccgt ggccacggcg gaaaccagcg agcccgagcg
     1921 ttcgctgctc gtcactcgcg aagcgctgac gggcaaggag ttgtttcgcc gcacagggga
     1981 cgaaggccgg acgttcggcg aagtggcgca ggacccgcgg cggccgctgg tcatggccag
     2041 ctacagcgac ggcgcccagc tcgcggtctt cgatgtggcc agcggacagc ggaccgcgct
     2101 ggccgagggc gcctcgataa cctcgccgcg gtttggcccg gacggctcaa tctggtacgc
     2161 ctgcgaggcc ccgccggcat tcttgcgccc ggcatccgca cagatcgtgc aggcagagct
     2221 gccggatttg acaccgcggc cgttctggat gaatcgtttc gcacaattca gccaccggat
     2281 cagtgccgtg atggcgctag tcgtcgatca ggacgcgatt gccttcgcga cgggcgacaa
     2341 acgcctgcac gtcttccatc gtgacacgcg acaggaagtc gcctcgctgc cgctcatttc
     2401 gactgcccgt gcgctgacgc tcgattctgg cggcaagtgg ctgctgtgcg gcgacgacgc
     2461 cggcatgctg acggtgtttc gttttccttc cctggaaagg gcttccgcgc acgctgtgca
     2521 cgacgcggcg ctgactgcta tcgcaacggc ggagaatggc ttgcttgcaa ccggcggtcg
     2581 cgaccggacg ctgcgcctct ggcgtttcgc ggccggagcg gtagagcccg tgctggccac
     2641 cggtcccttg ccgcagtcac ccgcgcaaat gcacatgacg gcagacggcc agtacctcgc
     2701 cctgctcctg gaaaacgacg ccgccgtccg ctggatcgat ctcgccggcc tgcaagccga
     2761 gctgtcgcgc cacggtttgg gatggtagct caccctcctt tacaagtgtc cgctgaatta
     2821 ttgagcagcg cgttcagggc aatcgccgga tggaccgcgg ctgtggccgc aaaatgggcc
     2881 agttgatgcc ggcaagagaa tcccgaggcg ataatggtcg ttccggatgc ggcctcgcgg
     2941 acggcgggga agagccgtcg ctcgccgacg gcctgcgaca gttcgtaatg ctcgcgctcg
     3001 tagccgaacg agccggccat gccgcagcag cccgcgtcca gatcgacgac ctcgcagccg
     3061 ggaattcgca tgagcagccg ctgcgcgggg cctgttccga cgagagcctt ctgatggcag
     3121 tgcgcgtgta gcagcacctt cggcggaccg gcttgcagtg gtaaggcatc gccggcttgc
     3181 aatgcccatt cctcgaaagt gaggcaagcc tgagacacca gccgcgcatc gcgctgctgc
     3241 tcgccgcgga gcaagtgcgg atggtcgtcg cgtaccgcgg aatagcagcc cggctcgcag
     3301 aacacgatcg gcagcccgcg cagggccagc ggcaacagcg cgtgcaccgt tttttcggct
     3361 tgccgccgcg catcgtccag gaatcccttc gaaatcagcg gccggccgca gcaaactcgc
     3421 ggcggcaccg tgacctgcca tcccgcggct gttgccagtt ccaccacggc catcgggatt
     3481 gccggttcgt gaaaattcgt gaaggtatcg gcaaacagcg ccagtcgccg gtcgtctggg
     3541 acgtttcggc cggcgagcgc cgtttgcatc tgccaccact ccagaaacgt ttgctgagca
     3601 aacacgggcg gcaagcgacg gcggtcgaga tcgaaccacg cttccgccag ccagcggacc
     3661 ggccggctcg ctgccagcca atttgacagt ggcgcgaatc gatggcccca aatggcaagc
     3721 tgctcgacat tggccagcag ccgggagcgc cattttgtcc cgtgccctag gcggtactga
     3781 tggaggaact cgctcttcat ccgcgccaga tcgacccccg tcggacactc gctcttgcag
     3841 gccttgcact ccaggcagag atccaggacg ggatagagcg cgggatcggt caggccgatc
     3901 ggcccgagct ggcctgagat cgccagccgc aagacattgg cccggccccg cgtggagtca
     3961 ttctcgtccc gggtcgccat gtacgagggg cacatcgttc cgctgagctt cttgcggcag
     4021 gccccgacgc cgccacactg ctcggccgct tgcgccaggc caccgaagtc cgagaagtcg
     4081 aaggcggttt cgacgggccg agtctggtag cgcgggccaa atcgcaagtt ggcggtcagc
     4141 ggctgcgctt cgacgatctt gcccgggttg aagagcccgc cgggatcgaa cgtctgcttc
     4201 agctcgcaga aagcctggta cagcgccggg ccgtacatct tctgctgga
//
LOCUS       DASRJB010000633         6840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1310348, whole genome shotgun sequence.
ACCESSION   DASRJB010000633 DASRJB010000000
VERSION     DASRJB010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310348"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFB80_16785"
     CDS             complement(<1..221)
                     /locus_tag="VFB80_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZN35488.1"
                     /translation="MFTGLVEERGTLASAEKSGDRQTGLSLRLTVKAPLVAQDAQIGD
                     SIAVNGCCLTVVKKSRQTLSFDAGSETLRR"
     gene            479..1363
                     /gene="argB"
                     /locus_tag="VFB80_16790"
     CDS             479..1363
                     /gene="argB"
                     /locus_tag="VFB80_16790"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZN35489.1"
                     /translation="MEEAIQKADTLIEALGWIRRFRDKITVIKLGGSVMEHENALRHV
                     LLDIVFMETVGMRPVVVHGGGAAIDRAMDAAGLVPRKIKGRRYTDDATLKIVEDVLAY
                     QTNEHLAGQIEELGGRAMPLNFRTTNVLFGERITLPGDNGQPIDLGHVGQVTRVDRTT
                     IENLCYAGQVPVIPSMALDETTGGKLNVNADTAATAVATALGAEKLMYMSDVNGVRRD
                     KSDPGSIIHSLTDGEARELIRTGVIDSGMIPKVEACLDTLDRGVRKVHIIDGRLRHSL
                     LLEIYTTSGVGTEIVKGM"
     gene            1463..2680
                     /locus_tag="VFB80_16795"
     CDS             1463..2680
                     /locus_tag="VFB80_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN35490.1"
                     /translation="MATAQEVESHLSSSETVELFKRYVIGNYNRYPVNLVRGEGSLVW
                     DAEGSRYLDLFPGWGCNLLGHCPPHVVRAVQDQVATLIHVPNTWHMDVQGRWAQMLSE
                     RSFGGQAFFCNSGAEANEAAIKLARLHGKGRYKIVTFSGGFHGRTLGALTATAQPKYH
                     EGLGPLVAGFLYAPFGDLEAAAKLIDDETCAVLVEPIQGEGGVRIPPAGFLQGLRKMC
                     DQRGLLLIFDEVQTGCGRTGDWFAYQHFGVTPDAMTLAKALCGGIAGGALLAKPEIAP
                     ALRPGMHAATFGGNPIAARAGIATLEMIEQDGLLDNAKQLGELFRSRFTALAEECDVI
                     REVRVCGVMVGLELAVDGTPVVKACLERKLLVNCTHGTVIRLLPAMNLTEEQAEEGCE
                     ILADVIRKLPRPA"
     gene            2781..3698
                     /gene="argF"
                     /locus_tag="VFB80_16800"
     CDS             2781..3698
                     /gene="argF"
                     /locus_tag="VFB80_16800"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZN35491.1"
                     /translation="MRDFLNVFDLSTEEIHRVFAISKELKDKFQAGLREPLLAGRVMA
                     LLFDKPSLRTRVSFECAMAHLGGSALFLGGDVGWGKREAAGDFAQVIGQYVDVVVCRT
                     STHSRIEELANFCNVPVINGLTDAAHPCQALADLFTLDEIHSPLEGKRLAYVGDANNV
                     ARSLAIACGKLGVEFAIASPPEYQFTREFLEELATKVEGVKIFQTADPREAVRGAMAV
                     YTDVWASMGQESEAEIRKSIFAPYQVNSNLMEQAPEGAVFLHCLPAHRGEEVTDEVID
                     GHYSAVWVQAANRLHVQKGLLAWLLNAID"
     gene            3705..4418
                     /gene="rph"
                     /locus_tag="VFB80_16805"
     CDS             3705..4418
                     /gene="rph"
                     /locus_tag="VFB80_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZN35492.1"
                     /translation="MRKPTELRPVKIKRHYTRMSPGSVLFQAGNTTVLCTASVEAKVP
                     DWLKGKGKGWITAEYNMLPHSTSPRKARERAKLDGRTTEIQRLIGRSLRSVADLAALG
                     ERSILVDCDVLEADGGTRTASVTGAFVALVDCLRSVQKELPDPSRFPLTGSVAAVSVG
                     IVGGKPALDLDYAQDVEADVDMNVVITGAGRFVEIQGTGEEATFGDDDLAALLKLAKA
                     GIRQLAGLQQAALGKSWPF"
     gene            4505..5224
                     /locus_tag="VFB80_16810"
     CDS             4505..5224
                     /locus_tag="VFB80_16810"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN35493.1"
                     /translation="MLKFLKHSLRQQRLRSARVVDASGPCTSLVSGWAMVTAAIDASQ
                     PVYSFGVGDTVFWDLHMIRRFGVTVHAFDPTPASIAWVARQQLPPQFIFHDFGISNFD
                     GELDFHPPRRIGNTHYSQEKRWRPFDRRPPVSGRVYRLATIMRQLGHDRLGVLKLDVE
                     GSEFEAIPDLIASGIEVDQLLVEIHYHFPSRSFAEGMQLVRQLKANGFVCVFASPRGL
                     EFSFVREGLKLGAAAPLARAA"
     gene            complement(5235..5438)
                     /gene="csrA"
                     /locus_tag="VFB80_16815"
     CDS             complement(5235..5438)
                     /gene="csrA"
                     /locus_tag="VFB80_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HZN35494.1"
                     /translation="MLVLSRKRDERIVIGDNIVITVVEVRGDKVRLGIEAPTEVPVHR
                     QEVLDAMRRNAGQTPPSVPPTVA"
     gene            complement(5716..>6840)
                     /locus_tag="VFB80_16820"
     CDS             complement(5716..>6840)
                     /locus_tag="VFB80_16820"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HZN35495.1"
                     /translation="LVQFPLSKELAAAGPLRLIETTGGKETPALAQVDPLGNRLWWVA
                     GGVLAAGAKRTYRLEAGESPAAPGIAVAESERAVAASCDSRPLLQYAKAHVEPASRIN
                     PRYGRSAHLHPVRTPSGAIVTDEFPPDHLHQSGIFLAFTKTEFKGHDVDFWNLAGGKG
                     RVRCKSVKGVSSGPVFGRILAEHEHVDLTAADATAGVGAKSGGDVALNETWEVQIWNV
                     GMKSGYWLLDITSQITCASDQPVKLPEYHYGGMAIRAARSWTPNEVRFLTSEGDERVK
                     GNQTRPRWCRVSGLVDGKPAGITLLTHPGNFRFPEPLRVHPTMPYMVYTPSFLGDWEI
                     KPGTPHVSRYRFVVHDGELSAEILERLWQDYAQPLTASGT"
BASE COUNT         1193 a         2221 c         2227 g         1199 t
ORIGIN      
        1 cggcggagcg tctcgctgcc ggcgtcgaac gagagcgtct ggcgggactt tttgacgacc
       61 gtcaggcagc agccattgac ggcgatgctg tcgccgatct gggcgtcctg ggccaccagc
      121 ggggctttca cggtcaggcg caggctgagg ccggtctggc ggtcgccgct cttttccgcg
      181 ctggcgagcg tcccccgctc ctcgacgagt cccgtaaaca tgggttcatg atagctgcga
      241 agcccgcggg cagaaactgc gaagcccgcg ggcagagaca cagttgcgcg aacccgacag
      301 gggcgagcgg cgtctggtct gtccatgaat tccgccgccg cgtcgttgac agcttggggc
      361 cacgacacaa caattcacgg tttgcgttcg ccgtcagggc aagccgaggg cttgccctcc
      421 gttgtttacg gctggcctgt tcgcttgccc gttttcctgg cggaggagtt ttgctgcgtt
      481 ggaagaggcc attcaaaagg ccgatacgct gatcgaggcg ctcggctgga ttcgccgctt
      541 tcgggacaag atcacggtca tcaagctcgg cgggagcgtg atggagcacg aaaacgccct
      601 gcgccacgtg ctgctcgaca tcgtcttcat ggagacggtt gggatgcggc cggtggtggt
      661 ccacggcggc ggcgcggcga tcgaccgggc catggacgcg gcgggcctcg tgccgcggaa
      721 gatcaagggc cgccgctaca ccgacgacgc gacgctgaag atcgtcgaag acgtgctggc
      781 ctatcaaacg aacgagcatc tcgcgggcca gatcgaagag ctcggcggcc gggcgatgcc
      841 gctgaacttt cgcacgacga acgtgctctt cggcgagcgg atcacgctgc ccggagataa
      901 tgggcagccg atcgatttgg gccacgtggg tcaggtcacc cgagtcgatc gcacgacgat
      961 cgagaatctt tgttacgccg ggcaagtgcc ggtgattcct tcgatggccc tggacgagac
     1021 gacgggcggc aagctcaacg tgaatgccga cacggcggcg accgcggtgg cgaccgcgct
     1081 gggggccgag aagctgatgt acatgagcga cgtcaacggc gtccgccgcg acaagagcga
     1141 ccccggctcg atcatccact cgctcacgga cggcgaggcc cgcgagctga ttcgcacggg
     1201 cgtgatcgac tcgggcatga ttcccaaggt cgaagcctgc ctcgacacgc tcgaccgcgg
     1261 cgtccgcaaa gttcacatca tcgacggccg cctcaggcac tcgctgctct tggagattta
     1321 cacgaccagc ggagtgggga cggagatcgt caaaggaatg tagggaccgc cctccgaggc
     1381 gttcctgtag ccgaactcgc cagagttcgg aggaatgcga ccgcggccga attctggcga
     1441 attcggctac aagaagacat tcatggccac cgctcaagaa gtcgaaagcc accttagttc
     1501 ctccgagacg gtcgagctct tcaagcgcta cgtcatcggc aactacaacc gctacccggt
     1561 gaacctcgtc cgcggcgaag gctcgctggt ctgggacgcc gaggggagcc gctacctcga
     1621 cctttttccc ggctggggct gcaacctgct cggccactgc ccgccgcacg tcgtccgcgc
     1681 ggtgcaggac caggtcgcga cgctgatcca cgtgcctaac acctggcaca tggacgtgca
     1741 gggccgctgg gcccagatgc tctcggagcg gagctttggc ggccaggcct tcttctgcaa
     1801 cagcggcgcg gaagccaacg aagcggcgat caagctggcc cggctgcacg gcaaggggcg
     1861 ctacaagatc gtcaccttca gcggcggctt tcacggccgc acgctcggcg cgctgaccgc
     1921 aaccgcacag cccaagtatc atgaaggact cggaccgctc gtggccgggt ttctctacgc
     1981 tcccttcggc gacctggaag cagccgcgaa gttgatcgac gacgagacct gcgcggttct
     2041 cgtcgagcca attcaaggag aaggcggcgt gcgaattcct cccgcgggct tcttgcaggg
     2101 cctgcggaaa atgtgcgatc agcgagggct cttgctcatc tttgacgagg tgcagacggg
     2161 ctgtggacgc accggcgact ggttcgccta tcagcatttc ggcgtcacgc ccgacgcgat
     2221 gacgctggcc aaggccctct gcggcggcat cgccggcgga gcactcttgg ccaaacccga
     2281 gatcgccccg gcgctgcggc cggggatgca cgccgcgacc tttggcggca atccgatcgc
     2341 cgcccgcgct gggatagcca cgctggagat gatcgagcag gacgggctgc tcgataacgc
     2401 caagcagctc ggcgagctct tccgcagccg attcaccgcg ctggccgagg agtgcgacgt
     2461 gattcgcgaa gtccgcgtct gcggcgtgat ggtcggcttg gagctggctg ttgacggcac
     2521 cccggtggtc aaggcgtgcc tggagcggaa gctgctcgtc aactgcacgc acgggaccgt
     2581 gatccgcctg cttccggcga tgaacctgac cgaggagcag gccgaggagg gttgcgagat
     2641 tctggccgat gtgatccgca agctgccgcg gccggcgtag cgcccctggt gctttgaagg
     2701 tggcacgggg ggccgcgtgg ccccccgctg gcgttttggg ctcgttcgtt tttttgctgt
     2761 cttatcgagg ggcacgcccc atgcgagatt tcttgaacgt cttcgatctg tccaccgagg
     2821 agatccaccg ggtctttgcg atctccaagg aactcaagga caagttccag gccgggctgc
     2881 gcgagccgct gctcgccggc cgcgtaatgg ccctcctgtt cgacaagccc tcgctccgca
     2941 cgcgggtcag cttcgaatgt gccatggccc atctgggcgg atcggcgctg tttctcggcg
     3001 gggacgtcgg ctggggcaag cgcgaggcgg ccggcgactt tgcccaggtg atcggccagt
     3061 acgtcgatgt cgtcgtctgc cgcacgagca cgcacagccg gatcgaggaa ctggccaatt
     3121 tttgcaacgt gccggtgatc aacgggctga ccgacgcggc ccatccctgc caggccctgg
     3181 ccgacctgtt cacgctcgac gagatccaca gtcccctgga gggcaagcgg ctggcctacg
     3241 tcggcgacgc gaacaacgtg gcccgcagcc tggccatcgc ctgcggcaag ctgggagtcg
     3301 aattcgccat cgcctcgccg ccggagtatc aattcacccg cgaattcctg gaggaactgg
     3361 ccaccaaagt cgagggtgtg aagatcttcc agacggccga tccccgcgag gcagtccgcg
     3421 gggcgatggc cgtctatacc gacgtctggg cgagcatggg gcaagagtcc gaagccgaaa
     3481 tccgcaaatc gatcttcgcc ccctatcagg tcaactccaa cctgatggag caggcccccg
     3541 agggggcggt gttcctgcat tgcctgccgg ctcaccgcgg cgaggaagtc accgacgaag
     3601 tcatcgacgg ccactacagc gccgtctggg ttcaggccgc caaccgcctc catgtccaaa
     3661 agggcctgct ggcctggctg ctgaatgcga ttgactgatt catcatgcgc aagcccaccg
     3721 agctccgccc cgtcaagatc aagcggcact atacgcggat gtcgcccggc agcgtgttgt
     3781 tccaggccgg gaacacgacc gtcctctgca cggccagcgt cgaggcgaaa gtgcccgact
     3841 ggctcaaggg caaaggcaag ggctggatca ccgccgaata caacatgctg cctcacagca
     3901 ccagcccgcg caaagcccgc gagcgggcca agctcgacgg ccgcacgacc gaaatccagc
     3961 ggctgatcgg ccgcagcctg cgctcggtgg ccgatctggc ggcactcggc gagcggtcga
     4021 tcctcgtgga ttgcgatgtg ctggaggccg acggcggtac gcggaccgcc agcgtcaccg
     4081 gcgcgttcgt ggccctggtc gattgcctcc ggtccgtgca aaaggagctg cccgatccga
     4141 gccgctttcc gctgaccggc agcgtcgccg ccgtcagcgt gggcatcgtc ggcggcaagc
     4201 ccgcgctcga tctcgactac gcccaggatg tggaggccga cgtcgatatg aacgtcgtga
     4261 tcacgggcgc tggccgcttt gtcgagattc agggcacggg cgaggaagcc accttcggcg
     4321 acgacgatct cgccgcgctg ctcaagctcg ccaaggcggg catccggcag ctggcgggcc
     4381 tgcagcaagc ggccctcggc aaatcgtggc cgttctagcg ggtttttcag tggctaattg
     4441 ttatctctct agtcgatatg ccccgcaggg agtaacatcc gcgccaaatc gaccagctag
     4501 cagcatgctg aagttcctca agcactcgct ccgccagcag cgcctgcgga gcgcccgcgt
     4561 ggtggacgcc tcgggcccct gcacgagcct ggtcagcggc tgggcgatgg tcaccgcagc
     4621 gatcgacgcc agtcagccgg tctattcctt cggggtcggc gacacggtct tttgggacct
     4681 gcacatgatc cgccggttcg gcgtcacggt gcacgcgttc gatcccacgc cggcgagcat
     4741 cgcctgggtg gcgcggcagc aattgccgcc gcagttcatc ttccacgatt tcggcatctc
     4801 gaacttcgac ggcgagctcg acttccaccc gccgcgcagg attggcaata cgcactattc
     4861 gcaagagaag cgctggcggc ccttcgatcg ccgcccgccg gtgagcggcc gcgtctatcg
     4921 gctggcgacc atcatgcggc agctcggcca cgaccgcttg ggcgtgctca agctcgacgt
     4981 cgagggcagc gagttcgaag cgatccccga tctgatcgcc agcggcatcg aggtcgatca
     5041 gctcctggtc gaaatccact atcactttcc ctcgcgatcg ttcgcggagg gaatgcaact
     5101 ggtgcgccag ctgaaggcca atggtttcgt ctgcgtcttt gcttcgccgc gcggtctgga
     5161 attcagcttt gtccgcgaag gtctgaaatt gggcgcggcg gccccgttgg cccgagccgc
     5221 ttgagattga agtgctaggc gacggtcggc ggcacgctgg gcggcgtctg gccggcattg
     5281 cggcgcatgg catccaggac ctcttgccgg tggacgggca cctcggtcgg cgcttcgatc
     5341 cccagccgca ctttatcgcc gcgcacctcg accactgtga tgacgatatt gtcgccaatc
     5401 acaatccgtt catcccgctt ccgcgatagc acaagcatga cagcacagcc tcgtctcacg
     5461 atgtaaacgc atgcaaacca ttaccgcgcc taattttaga atgggtgttg gttcggttaa
     5521 gggttggacg gttggaaaag caagtgcgcg agcgaaccca ggctcgaatt cgcccaacat
     5581 ttgccctcgt cgatatgtcg caactgcttc ggcggcaagc acttagggcg agactctcgc
     5641 ctctcgactc gcgcgcagcg ggaagcgcga atagccagtc aacatcgccg cctgccgcca
     5701 gacagttcgg aaatcttacg tgccactcgc cgtgagcggc tgggcatagt cttgccagag
     5761 ccgctccagg atttctgcag agagctcccc gtcgtggact acgaagcgat accgcgaaac
     5821 gtgcggcgtg cccggcttga tctcccagtc gccgagaaac gagggcgtat agaccatgta
     5881 gggcattgtg gggtgaactc gcagtggctc gggaaagcgg aaattgcccg gatgggtcag
     5941 gagcgtgatg ccagccggct tgccatcgac cagtccgctc acgcggcacc accgcggacg
     6001 ggtttggttg cccttgacgc gctcgtcccc ttccgaagtc agaaaccgaa cctcgtttgg
     6061 cgtccatgaa cgcgcggccc ggatcgccat gccgccatag tgatactccg gcagcttgac
     6121 cggctggtcg ctggcgcaag tgatctgcga ggtaatgtcc agcagccagt agcccgactt
     6181 cattccaacg ttccagatct gcacctccca ggtttcattc agggcgacgt cgcctcccga
     6241 ttttgcaccg acgccggccg tcgcgtcggc ggccgtcaga tcgacgtgct cgtgctccgc
     6301 gaggatgcgg ccaaagacgg gaccgctgct gacacccttc accgacttgc aacggacgcg
     6361 gcccttgccg cccgcgaggt tccagaaatc gacgtcgtgc cctttgaatt ccgttttcgt
     6421 gaacgccagg aaaatgccgc tctgatgcag gtggtccggc gggaactcat cggtcacgat
     6481 cgccccgctg ggagtgcgga cgggatgcag gtgagcgctg cggccgtagc gcggattgat
     6541 tcgcgaagcc ggctcgacgt gggccttggc atattgcaag agcggccgcg agtcgcagct
     6601 ggctgctacc gctcgctccg attcggcgac ggcaatgccc ggggctgccg gcgactcgcc
     6661 ggcctcgagc cgataggtcc gcttcgctcc ggccgccagc acgccgccgg cgacccacca
     6721 cagccggttg cccagcggat cgacctgggc cagggcgggc gtctcttttc cgccggtcgt
     6781 ttcaatcaga cgcagcggcc cggccgccgc cagttccttg gacagtggaa actgcaccag
//
LOCUS       DASRJB010000634        14178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_750353, whole genome shotgun sequence.
ACCESSION   DASRJB010000634 DASRJB010000000
VERSION     DASRJB010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14178
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750353"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFB80_16825"
     CDS             <1..1479
                     /locus_tag="VFB80_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZN35496.1"
                     /translation="CVNFVGVDVNSASPALLRYVSGMNALTARRVYEHRRDKGPFKSR
                     DDLKQVQGFGDQTFVQAAGFLKVIGSENPLDATWIHPESYDIARRVLEKMGAGVADLA
                     HVVPAPVKEEPKKEFAAELVSRESGVGAQESEARESEAPAEPAADSQTEGKSEGETVP
                     PGYEVVSTTSATVPFSDILAALRPPVAPSLPPEPEVAPPPPEPAATPQAPPPPPRNPI
                     AEKAQAVNVQQLAEELGVGTHLLRDILNSFSRPALDPRDSLSAPVFRRGIMKLEDLAP
                     AMELSGTVLNVVDFGVFVDIGLSDSGLVHISRLADRFIKDPHEVVGVGDILKVWVVDI
                     DKSRRRVSLTAIPPGTERPPRPPRDQQQRPPRPPRQRQGGQGGRPQQGGQPQNQHQGG
                     PATGAPPQGQRPPRPAGGGGGRRDQRRGGRPQHDHPQTIERPPTKPKKFKPITKAQEE
                     GREPMRSFGDLQQLFEKKRQKPSPPEEGSAEPAGEQPPPETQ"
     gene            1725..4331
                     /locus_tag="VFB80_16830"
     CDS             1725..4331
                     /locus_tag="VFB80_16830"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZN35497.1"
                     /translation="MAKLDSPRLRRCISGGGLRCLAASAALLLVTLAAGTLAASEADL
                     AIPDLREGHFKNLGGISAWNLLAIGALVICGTLGISLYQLYQIKKQPAHKSMLDVAEI
                     IFQTCKTYLIQQGKFLLMLFVLIAIAISYYILGASHEAEPGAVSEVAAAAAEAGPKLS
                     KPVTLALVLLFAIVGMAGSYSVAWYGIRVNTYANARTAFASLRGEPWDVVNIPLRAGM
                     SIGLFLISLELVMMVIILLFVPRQVVGVCFLGFAIGESLGASALRIAGGIFTKIADIG
                     SDLMKIVFNVKEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTGVALISFIVLAI
                     TQASGVTNYLDLQAKLIVWIFAMRFLMDFMSGVSYFINQAISEAKYKGLKEFNFEDPL
                     TRLIWIASILCITTSFGMSWLLIRDLSVVKGGVTTALPHLWWQLATIISCGTLAAVLI
                     PEFTKVFTSSHSKHVHEIVTASREGGASLTILSGIVAGNFSAFWKGMLIAGLMGVAYF
                     VSMPMGFLGIEQSLGDVMGPHASIFAFGLVAFGFLCMGPVNIAVDSYGPVTDNAQSIF
                     ELAQTEHVPNIKQEIQRDFGFVPDFAQGKHYLEANDSAGNTFKATAKPVLIGTAVVGA
                     TTMIFAIILLLEKTIVDGKSLLQLSLTDAPVLLGFICGGSVIFWFSGASMQAVTTGAY
                     SAVEFIKKNMKLDKKEADLEDSKTVVRICTEYAQKGMWNIFIALMTITLAFAFFDPNF
                     FVAYLISIAVFGLFQAIYMANAGGAWDNAKKLVEVDLKEKGTALHAATVVGDTVGDPF
                     KDTTSVALNPIIKFSTLFGLLAVEIAVGRLLESHAKQTTNYIPFVGIFMLAVALYFVW
                     RSFYAMRIPEKV"
     gene            4889..6565
                     /locus_tag="VFB80_16835"
     CDS             4889..6565
                     /locus_tag="VFB80_16835"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZN35498.1"
                     /translation="MRCLLLVALVLGIVSGPPAAAQDATYDLLIQGGRIVDGSGNPWF
                     TGDVAVAGGKIVAVGRVPAGKAAKQIDAAGLIVAPGFIDMHSHSDTLLLEDGHAQSKI
                     RQGVTTEVLGEGSSAGPRVGKMSPQKVLAGGKPVTWTTLGDYFAAIDQAGVSVNVVSY
                     VGIDNVWESVMGESHARPSSEQFKEMQKLVEQAMQEGAIGLSTMLTMPPGSLATTDDL
                     VLLCEPVARFGGLYSTHNRNEGTGVFDAIREAIAVGERAKIPVDIIHVKIADQQYWGR
                     MKEIVELIEAARARGVNVQTNIYPYTRGNNDLASIIPPWAHEGGKSQFLARLKNPADR
                     ERMKKEIRGGIPGWYNHFTAVGGDWSRMLISGRSQYEGQTMDRVIALRTMNLTKKPDE
                     LDVLFDLLIDEEGSVPTVYAHHTEEDMNYALRQPWCSIGSDGSAYATEGSLRRGNPHP
                     RNFGTFPRVLGVYARERKLLTLEEAVRKMTSLNAAKIGLRDRGEVREGLAADLCLFDP
                     AKVIDRATYTDPFHYNEGIEYVVVNGQLVLDRGTHTGARPGRALRKQPLK"
     gene            complement(6569..7246)
                     /locus_tag="VFB80_16840"
     CDS             complement(6569..7246)
                     /locus_tag="VFB80_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35499.1"
                     /translation="MRLRILIICVVTLIAFGPDVALAKGGKGGGKGGGGRPSFAGGKG
                     GGGSTQQSKGGGGKLATAPSGKEHKEFKEHKEHKELKEHKDHKDKLADSGKTNDKLAK
                     KDGSFPTDSGETYNKKQKQLEIEQRNRDKRLAQAEHLRQIAERNGNEQLAANADRMEA
                     FAHDHYARRVAQLEKFGVTDPALDPGGVIDPLNDPAGEFPELPLVPDLPPLPELPRLP
                     LLPRLLP"
     gene            complement(7420..7851)
                     /locus_tag="VFB80_16845"
     CDS             complement(7420..7851)
                     /locus_tag="VFB80_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35500.1"
                     /translation="MAIKYGRAKHVESVTADDCVKHPIWVMAHRSKFHEEGVQPIVNK
                     VNVDREVLRARVPIIAVQIEGTDIYGLACYDHDSHDFSSLALLIGGKWVDAFDKRTRL
                     EFPVTFAVLPQIMGQAGVRFVWKSRKAALQREAARRSVKRG"
     gene            complement(7879..8187)
                     /locus_tag="VFB80_16850"
     CDS             complement(7879..8187)
                     /locus_tag="VFB80_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZN35501.1"
                     /translation="MMLGHMVYFTLKDRSPTAVDKLVAACRKYLTGHPGTVFFAAGTL
                     VPDLTRPVNQQDFDVALQVVFDSREAHDAYQVAPRHVQFIEENKPNWERVRVFDAYVS
                     "
     gene            complement(8213..9145)
                     /locus_tag="VFB80_16855"
     CDS             complement(8213..9145)
                     /locus_tag="VFB80_16855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN35502.1"
                     /translation="MHVELVRTVTRDGLRLDGAFHLPLPLGEGRGSSSSLDPHPHPLP
                     KGEGTAAVLLHGVAGNFYSSSTFEPLVPRLRKLGLAVLSVNTRGHDSVFGATLGTVRQ
                     RLGAAYETVDDCLLDIAAWIDLLAARGHTWVVLIGHSLGAIKAVYGMAHDKWPAVSAV
                     VAVSPPRLSCAAFQNSPDSSSPFFESLSTAERMVAEGRGEELFTSKFPFPLLITAAGY
                     IDKYGPAERYNLLRFAHELPCPALFTYGSKELAGGGIAFDGLPEALQSLPNSSRRQVA
                     VIEGADHIYTGLGEELAAAITDWLAPSIMAATST"
     gene            complement(9149..9631)
                     /locus_tag="VFB80_16860"
     CDS             complement(9149..9631)
                     /locus_tag="VFB80_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35503.1"
                     /translation="MASEQRLFTPVFSIDVSADHHAAAANTKIDANELIVGLLKQLVD
                     GQQREIKLLEEIAHYVGLNHKQRQQEIANWKQANPDLARSCRIATEALAKVQNDLIHK
                     LTEDAADNHESMVDSEFALAEFVDRFGPRMAHLGGMLHMVSTLSAQKEAPPAETAQEP
                     "
     gene            9827..10339
                     /locus_tag="VFB80_16865"
     CDS             9827..10339
                     /locus_tag="VFB80_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35504.1"
                     /translation="MSASAKQVPCLGFLTVVEHAELGLLGGYLLLNLAGRPLEFHCTA
                     PVKPNRAQEILYGPTLRPFLYGEQIGQTLLARSKLTPLVACTDSESVLAARDFTETPL
                     VLVREGEAAELRSFRLGRNHVTATPGYGSDQQAILAAWPAQADHLDLCEPFVRIREAL
                     EEAQKAARAA"
     gene            10386..12107
                     /locus_tag="VFB80_16870"
     CDS             10386..12107
                     /locus_tag="VFB80_16870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN35505.1"
                     /translation="MPPSALTIDLDLAARPLATDFALPLPAPIVVRSQPLGFRAEAPA
                     TITVKTPSSRPRVFSFTFPEAAPSLRLSLPPSLPPAAPRPKLDGKPPTRIKPPGDIIK
                     LEDRLYYVLQPPLETLLASDALHFPFEPFPYQYQGVAFLYPRYSAVLADEMGLGKTMQ
                     AITAIRMLLRSGEIRSVLLVCPKPLVTNWRREFNLWAAEIPLTIIEGDAAKRRWQWTQ
                     EEAPVKIANYELLMRDKGVLDESLAFDLVVLDEAQRIKNTGSTTSQIARSIRRTRNWA
                     LTGTPIENTPDDLVGIFEFLSPGYLRSGTAPRRMAELVREHILRRTKDMVLTDLPPKM
                     FRDAELDLSPEQRATYELAENEGVMQLNAMGDQITIQHVFELVLRLKQICNFDPATGA
                     SSKLERLEADLEEVAASGKKAIVFSQWVKCLEILKHQLRRFGPLEYHGQVPSKQRDGV
                     LKQFQEDPSKHVILMSYGAGSVGLNLQFCEYVFLFDRWWNPAVEDQAINRAHRIGAAG
                     PVTVTRMLALATIEERINQVLEHKRELFAQIIGQNSAPPSLGLSQAEIFGLFNLRTPK
                     GVLTPAA"
     gene            12192..13379
                     /gene="sucC"
                     /locus_tag="VFB80_16875"
     CDS             12192..13379
                     /gene="sucC"
                     /locus_tag="VFB80_16875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZN35506.1"
                     /translation="MKIHEYQAKDIFRKSGVAVLRGKVAASAEEAAAAFKELGGPLAV
                     VKAQIHAGGRGKGTIKRNPQQRGVQLVRSAEEAGKVAKNLLGQELVTIQTGPEGKTVN
                     RVFVEEGCQIARELYLGIVLDRAVAKPVLMVSSEGGVEIEKVAAETPHLIFKEHFDPQ
                     VGLLPYQVRKLCYKLKLQGPTVASAEKFMRALCRLYVQLDCSLVEINPLVVTQSGELI
                     ALDGKLSFDDNAMFRHKDLAELRDLSEEDPAEVRASNTGLSYVKLDGNIGCLVNGAGL
                     AMSTMDIIKLHGGEPANFLDVGGGANEQQVTEAFRIILSDPNVKGILVNIFGGIARCT
                     TIAAAIVAASKAVGFKVPLVVRLEGTEVEEGKKILRESGVKLITADDLTDAAKKIVAA
                     VKG"
     gene            13411..>14178
                     /gene="sucD"
                     /locus_tag="VFB80_16880"
     CDS             13411..>14178
                     /gene="sucD"
                     /locus_tag="VFB80_16880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZN35507.1"
                     /translation="MSILINKSTRVICQGITGKAGAFHTKGCKDYGTQMVGGVTPGKG
                     GETVEGLPVFDTVIEAVEKTGANATMIFVPAAFTADAILEALDAGIKVICAITEGVPV
                     LDMVRVYELVRKSDAVLIGPNCPGVITPGECKIGIMPGYIHQPGKVGIMSRSGTLTYE
                     AVWQTSNLKLGQSTCIGLGGDPIVGTNYIDLFKRFQNDPQTEAILMIGEIGGTAEEEA
                     AAFVKEHISKPVAAFIAGRTAPPGRRMGHAGAIISGGK"
BASE COUNT         2544 a         4553 c         4579 g         2502 t
ORIGIN      
        1 tgcgtgaact tcgtgggcgt cgatgtgaat tcggccagcc ccgcgctctt gcggtatgtg
       61 tcgggcatga acgctctcac ggcccgccgg gtgtacgagc accgccgcga caagggcccc
      121 ttcaagagcc gcgacgacct caagcaagtg cagggctttg gcgaccagac attcgtccag
      181 gccgccgggt ttctcaaagt gatcggcagc gagaatccgc tcgatgctac ctggatccat
      241 cccgaaagct acgacatcgc ccgccgcgtc ctggaaaaga tgggcgctgg cgtcgccgac
      301 ctggcccacg tcgttcccgc gccggtgaag gaggagccga agaaggagtt cgcagcggag
      361 ctggtgagtc gggagtccgg ggtcggggct caggagtcgg aggctcggga gagcgaggct
      421 cccgccgagc cggcggctga cagccagacg gagggaaaga gcgaaggaga gacggttcca
      481 ccgggttacg aagtagtcag caccaccagc gcgacggtgc cattctctga catcctcgct
      541 gccctccgtc cccccgtcgc tccgtctctc cctcccgaac cggaagtcgc gccaccccct
      601 ccagagccgg ccgccactcc gcaagctcct cctccgccgc cccgcaatcc gatcgccgaa
      661 aaggcgcagg cagtgaacgt gcagcagctc gccgaggagc tgggcgtcgg cacgcacctc
      721 ttgcgcgaca ttctcaattc gttctcgcgg ccggccctcg atccgcgcga cagcctgtcg
      781 gcgccggtct tccgccgcgg gatcatgaag ctcgaggatc tcgctccggc gatggagctg
      841 tcgggcaccg tgctgaacgt ggtcgatttc ggcgtgttcg tcgacatcgg cctttccgac
      901 agcggcctgg tgcacatcag ccggctggcg gaccgcttca tcaaggaccc acacgaggtg
      961 gtcggcgtcg gcgacatttt gaaggtctgg gtcgtcgata tcgacaagtc ccgccgccgc
     1021 gtttcgctca ccgcgatccc gccgggcacc gagcgtccgc cgcgtccgcc gcgcgatcaa
     1081 cagcagcggc cgccgcgccc gccgaggcaa aggcagggcg gccagggcgg ccggccacag
     1141 cagggcggac agccgcagaa tcagcaccaa ggcggacccg cgaccggcgc tccgcctcag
     1201 ggtcagcgcc cgccccggcc tgccggcgga ggcggcggcc ggcgcgatca gcgccgcggc
     1261 ggccgtcccc agcacgacca tccgcagacg atcgagcggc ctcccaccaa gcccaagaaa
     1321 ttcaagccga tcaccaaggc ccaggaagaa ggccgcgagc cgatgcggtc gttcggcgac
     1381 ttgcagcagc tctttgaaaa aaagcggcaa aaaccaagtc cgccggaaga aggctccgcc
     1441 gagcccgcgg gcgaacagcc gccgccagag acccagtgaa ctctagcgga ctgggccgca
     1501 ttctagtgcg atttgcccgc cctcacgccg ggtcctgtgg ttgacccctc gcgcaccgat
     1561 acattacatt ttgcgtctta agatcgcgta aggattcacg ccaccgagga tgaccggtcg
     1621 tctcgcgtgg gccgctttgc ccgcgcttcg ttccgcgcta ttgaagattc cgcgtcgccg
     1681 atgactttgt gaaattcggc acaagcccct gaggattacg gaaaatggcg aagcttgact
     1741 ctccccggtt gcggcgctgt atttctggcg gcgggctgcg ctgtctcgca gcgagcgccg
     1801 ccctgctact agtcaccctg gctgccggaa cgctcgccgc cagcgaggcc gacctggcga
     1861 ttcccgatct gcgcgagggc cacttcaaga acttgggcgg aatcagcgcc tggaacttgt
     1921 tggcgatcgg cgcccttgtc atctgcggca cgctgggaat cagcctctac cagctctatc
     1981 agatcaagaa acaaccggct cacaagtcga tgctcgacgt ggccgagatc atctttcaga
     2041 cctgcaagac ctatctgatt cagcagggca aattcctgct catgttgttc gtcctgatcg
     2101 cgatcgccat ttcctattac atcctcggcg cgagccacga agccgagcca ggtgcggtgt
     2161 ccgaagttgc ggcggcggcc gccgaggccg ggccgaagct ctccaagccc gtgacgctgg
     2221 ccctggtgct gctgttcgcg atcgtcggca tggccgggtc atactcggtg gcctggtatg
     2281 ggatccgcgt gaacacgtat gccaatgctc gcacggcatt cgcctcgctc cgcggcgagc
     2341 cctgggacgt ggtgaacatc ccgctgcgag ccggcatgtc gatcggcctg tttttgattt
     2401 cgctggagct ggtgatgatg gtcatcatcc tgctcttcgt gccgcggcag gtcgtcggcg
     2461 tgtgcttcct cggcttcgcg attggcgaat cgctgggcgc ttcggccctc cgcatcgcgg
     2521 gcggcatctt tacgaagatt gccgacatcg gctcggacct gatgaagatc gtcttcaacg
     2581 tcaaggagga cgatccgcgg aatcccggcg tgatcgccga ctgcacgggc gacaacgccg
     2641 gcgactcggt tggcccgacg gcggacggct tcgagactta cggcgtgaca ggggtggccc
     2701 tgatctcgtt tatcgtgctg gccatcacgc aggcttcggg ggtcacgaac tacctcgatc
     2761 tgcaggccaa gctgatcgtg tggatcttcg cgatgcggtt tctgatggac ttcatgtccg
     2821 gcgtctcgta cttcatcaac caggccatct ccgaagcgaa gtacaaggga ctcaaggagt
     2881 tcaacttcga ggacccgctg acccgcctga tctggatcgc gtcgatcctc tgcatcacga
     2941 cgtcgttcgg catgagttgg ctcttgatcc gcgacctgtc cgtcgtcaaa ggcggcgtga
     3001 cgaccgcgct gccgcacctg tggtggcaac tggccacgat catcagctgc ggcacgctgg
     3061 cagccgtttt gatccccgaa ttcacgaaag tcttcaccag ctcgcacagc aagcacgtcc
     3121 acgagattgt caccgcctcc cgcgagggcg gcgcctcgct gacgatcctg tcgggcatcg
     3181 tggccggcaa tttcagcgcg ttttggaagg gcatgctgat cgccgggctg atgggcgtgg
     3241 cctatttcgt cagcatgcca atgggctttt tggggatcga gcagagcctg ggcgacgtga
     3301 tgggcccgca cgcctcgatc tttgcgtttg gcctcgtggc cttcggcttc ctttgcatgg
     3361 gcccggtcaa catcgccgtg gacagctacg gcccggtcac cgacaacgcc cagtcgatct
     3421 tcgaactcgc tcagaccgag cacgtgccga acatcaagca ggagattcag cgcgacttcg
     3481 gctttgttcc cgattttgcc cagggcaagc actacctcga agccaacgac tcggcgggca
     3541 acacgttcaa ggctacggcc aagccggtgc tcatcggcac ggccgtcgtc ggcgcgacga
     3601 cgatgatctt cgcgatcatc ctgctgctgg aaaagacgat cgtggatggc aagagcctgc
     3661 tgcagctcag cctgaccgac gccccggtgc tgctgggctt catctgcggc gggtcggtga
     3721 tcttctggtt cagtggcgcg tcgatgcagg ccgtcaccac cggcgcgtac tcggcggtcg
     3781 agttcatcaa gaagaacatg aagctcgaca agaaggaggc cgacctggaa gacagcaaga
     3841 cggtcgtccg catctgcacc gaatacgccc agaagggcat gtggaacatc ttcatcgccc
     3901 tgatgacgat cacgctggcc ttcgccttct tcgatccgaa cttcttcgtc gcgtacctga
     3961 tttcgatcgc cgtgtttggc ctgttccagg cgatctatat ggccaacgcc ggcggcgcat
     4021 gggacaacgc caagaagctg gtcgaggtcg atctgaagga gaagggcacc gcgctgcacg
     4081 ccgcgacggt cgtcggcgac acggtcggcg acccgttcaa ggacacaacc agtgtggccc
     4141 tcaacccgat catcaagttc tcgacgctgt tcggcctcct ggccgtcgag atcgccgtcg
     4201 gccggctgct cgaatcgcac gccaagcaaa cgaccaacta catccccttc gtcggcatct
     4261 tcatgctggc cgtcgccctg tacttcgtct ggcgcagctt ctacgcgatg cggatccccg
     4321 aaaaggtcta gccggcggtc cttagccggg accaaacctg gtcccggccg gcgaggaagc
     4381 aaaaccgagc gtatgtgtac tttggtacgc tatttgcagt aaagtttcac tgagtggtgg
     4441 actgccactt tgacaggtgg cgggcttggt tctttggaaa ttcgcagcgg gcggccgcct
     4501 gagcctggcg tgtgctgctg cgcgcagccg ggctggtcac gaatgcaggg cggtgcagcg
     4561 gcgaattatt cgccaccgcc atttcgcctg taagtgctga tgtcgtaagg cacttgcgag
     4621 cggcgaccgt gaattgttcg ccagatccgc aggtatcgag gcagattggc gaataattcg
     4681 cggccaaaag tgcggaaaag cctgcgaatt tcaccaatcg cggccgaatt attcgccacc
     4741 gagttgccgg acccggcgaa gaatgcgcgg gtcgagtagt tgcgacatcg gccgacctgc
     4801 ccaagacctg gccggcacgg ggctttgcag cgcgcggaag cggcgcgtta cattccggca
     4861 aattgctgtt tcaactagcg aggctgccat gcgctgtttg ctgcttgtcg ccttggtgct
     4921 gggaatcgtg tcgggcccgc cggctgccgc tcaggacgca acctacgatc tgctcattca
     4981 gggcgggcgg atcgtcgatg gcagcgggaa tccgtggttc acgggggacg tggcggtggc
     5041 cggcggcaag atcgtcgccg tcggccgagt cccagccggc aaggcggcga agcagatcga
     5101 cgcggccggc ttgatcgtcg cgcccggctt catcgacatg cactcgcact ccgacacgct
     5161 gctattggaa gacggccacg cccagagcaa gatccggcaa ggcgtgacga ccgaggtcct
     5221 cggcgagggc tcaagcgccg gcccgcgagt gggcaagatg tcgccgcaga aagtgctcgc
     5281 cggcggcaag ccggtcacct ggaccacgct cggcgactac tttgccgcca tcgaccaggc
     5341 cggcgtgtcg gtcaacgtgg tttcctacgt ggggatcgac aacgtctggg aatcggtgat
     5401 gggcgaatcg cacgcgcggc ccagctccga gcaattcaag gagatgcaga agctcgtcga
     5461 gcaggcgatg caggagggcg cgatcggcct ttcgaccatg ctgaccatgc cgcccggctc
     5521 gctggccacg accgacgacc tggtgctgct ctgcgagccc gtggccaggt tcggcggcct
     5581 ttattcgacc cacaaccgca acgaaggcac cggcgtcttc gacgccatcc gcgaggccat
     5641 cgccgtcggc gagagggcca aaatccccgt ggacatcatc cacgtcaaaa tcgccgatca
     5701 gcagtactgg ggccggatga aagagatcgt ggagctgatc gaggccgccc gggcccgcgg
     5761 tgtcaacgtg cagaccaaca tctatcccta cactcgcggc aacaacgacc tggccagcat
     5821 catcccgccc tgggcccacg aagggggcaa gagccagttc ctggctcgcc tcaagaatcc
     5881 cgcggatcgc gaacggatga agaaggagat tcgcggcggc attcccggct ggtacaacca
     5941 cttcacggca gtcggcggcg actggtcgcg gatgctcatc agcggccgct cgcagtacga
     6001 agggcagacg atggaccgcg tgatcgccct gcggacgatg aacctcacga agaagcctga
     6061 cgagctcgac gtgctcttcg acctgctgat cgacgaggag ggttcggtgc cgacggtcta
     6121 tgcccaccac acggaggagg acatgaacta tgccctccgg cagccgtggt gctcgatcgg
     6181 ctccgatggc tcggcgtatg cgaccgaggg ctcgctgcgg cgcggcaatc cgcacccgcg
     6241 gaactttggc acgttcccgc gggtgctcgg cgtttatgcc cgcgagcgga agctgctgac
     6301 cttggaagag gccgtccgca aaatgacctc gctcaacgcg gccaagatcg gcctccgcga
     6361 tcgcggcgaa gtgcgggagg ggctcgcggc ggacctgtgc ctgttcgatc cggccaaggt
     6421 catcgaccgc gcgacctaca ccgatccctt ccactacaac gaagggatcg agtacgtggt
     6481 cgtcaacggc cagctcgtcc tcgaccgtgg cacgcacacc ggcgcgaggc cggggcgtgc
     6541 gttaagaaag cagccgctca agtaacgatc aaggcagcag tcgcggaagc agcggcagcc
     6601 ggggcagctc cgggagcggc ggcaggtctg gcacgagcgg cagctcgggg aattcgccgg
     6661 cgggatcgtt cagcgggtcg atgacgccgc ccggatccaa ggccggatcg gtcacgccga
     6721 acttctcgag ctgggcgacg cggcgggcgt agtggtcgtg ggcaaaggcc tccatccggt
     6781 cggcgttggc ggccagttgc tcgttgccgt tccgctcggc gatctgccgc aggtgttcgg
     6841 cctgcgccag ccgcttgtcg cggttccgct gctcgatctc cagctgcttc tgctttttgt
     6901 tgtaggtctc gcccgagtcc gtcgggaagc tgccgtcctt tttggccagc ttgtcgttgg
     6961 tctttcccga gtcggcgagc ttgtccttat ggtccttgtg ctccttcagc tccttgtgct
     7021 ccttgtgctc cttgaactcc ttgtgctctt tgccggaggg agcggtagcg agcttgccgc
     7081 cgcccccctt ggattgctgc gtggatccgc cgccgccttt gccgccggca aagctggggc
     7141 gtccaccgcc gcctttgccg ccgcccttgc cgcccttggc caaggcgaca tcggggccaa
     7201 acgcgatcag ggtcacgaca caaatgatga ggattcgaag acgcatgggc gactactcct
     7261 ctgcggaatg ggatctggtg agttgctttc tctcccaatt gcaaagctat gtcgcccagc
     7321 agcccggcgc tgactcttgt agcgattgcg cagaagaaga ggcggccggg cccacgcgca
     7381 gcaggccgcc tgtcgcgatt gtgccgaatt cgccggccat taaccgcgct tgacggaccg
     7441 gcgggcggcc tctcgctgca gcgccgcttt gcgagacttc cagacaaaac ggacgccagc
     7501 ctgtcccatg atctgaggga gcacggcaaa cgtcacggga aattccagcc tggtccgttt
     7561 gtcgaaggca tcgacccact tgccgccaat caacagcgcg agcgaggaaa aatcatggct
     7621 gtcgtggtcg tagcacgcca ggccgtaaat atccgtgcct tcgatctgga ccgcgatgat
     7681 cgggacgcgc gcgcggagca cttcgcgatc cacattcacc ttgttgacga tcggctgcac
     7741 gccctcttcg tgaaacttgg agcggtgtgc catcacccag atgggatgct ttacgcagtc
     7801 gtccgctgtg acggattcca catgcttcgc gcggccgtac ttgatggcca tacgccgctc
     7861 cgttcagctg gcgccgatct agctcacgta ggcgtcgaac acccgcactc gctcccagtt
     7921 gggcttgttc tcttcgatga actgcacgtg ccgcggcgcg acctggtagg catcgtgggc
     7981 ctcgcggctg tcaaagacga cttgcagcgc tacgtcgaag tcctgctgat tcaccggccg
     8041 cgtcagatcg ggcacgaggg tgccggcggc gaagaacacc gtgcccggat ggcccgtgag
     8101 atacttccgg cacgccgcca ccagcttgtc gaccgccgtg ggagagcggt ccttgagcgt
     8161 gaaataaacc atgtggccaa gcatcatcga cagagttcct tgtcgagcga gttcaagtcg
     8221 aggttgccgc catgatactc ggagcgagcc aatcggtaat cgccgctgcc agttcctcgc
     8281 ccaggccggt gtagatatga tctgcgccct cgatcacggc gacctgccgg cggctgctgt
     8341 ttggcaacga ctgaagcgcc tccggcagtc catcgaaggc gatgcctcct ccggctagtt
     8401 ccttgctgcc gtaggtgaag agcgcggggc aaggcagctc gtgggcgaag cggagcaggt
     8461 tgtagcgctc ggccggtccg tacttgtcga tgtagccggc cgccgtgatc aggagcggga
     8521 acgggaactt gctggtgaag agttcctctc cgcggccttc ggcgaccatc cgctcggcgg
     8581 tgctcagcga ctcgaagaac gggctgctgc tgtccgggga gttctggaac gccgcgcacg
     8641 acagccgtgg cgggctgacg gcgacgaccg ctgacaccgc cggccattta tcgtgcgcca
     8701 tcccatacac ggccttgatc gcgccgaggc tgtggccgat cagtaccacc caagtgtggc
     8761 cgcgagccgc gagcaagtcg atccaggctg cgatgtcgag caggcagtca tcgaccgttt
     8821 catacgcggc gcccagccgc tggcgaacgg tgcccaaggt cgcgccgaac accgagtcgt
     8881 gcccgcgggt gttgacgctg agcacggcga gtccaagctt ccgcaaccgc gggaccagcg
     8941 gctcgaaggt gctggacgaa tagaagttgc cggcgacgcc gtgcagcagg accgctgccg
     9001 ttccctctcc ctttgggaga gggtgagggt gagggtctaa tgagctcgaa gaaccacgac
     9061 cctcgcccag agggagaggg agatggaaag cgccgtccag ccgcaggcca tcgcgggtca
     9121 cggtgcggac cagttccacg tgcatcggct acggctcttg cgccgtctcg gccggcgggg
     9181 cttccttctg agcgctgagc gtgctgacca tgtgcagcat gccgccgagg tgggccatcc
     9241 gcgggccgaa ccgatcgacg aactccgcca gggcgaattc gctatcgacc atcgactcat
     9301 gattgtcggc ggcgtcttcg gtcagcttgt ggatcaggtc gttctggacc ttggccagag
     9361 cctcggtggc gatgcggcag ctgcgggcca gatcgggatt ggcttgcttc cagttggcga
     9421 tttcctgctg ccgctgcttg tggttcaggc cgacgtagtg ggcgatctcc tccagcagct
     9481 tgatctctcg ctgctggcca tcgacgagct gcttgagcag gccgacgatc aactcgttgg
     9541 cgtcgatctt cgtgttggcc gcggcggcgt ggtggtccgc cgaaacgtcg atgctgaaaa
     9601 cgggcgtaaa caaccgctgt tcgctagcca ttcatcggac tccagaagct tcactcaata
     9661 tacacggcct gcggaaggtg tcgagccttt ggcggtctgt gccggttacg cgagctatgc
     9721 ggcagccgcg gcggctaaag acggcgcgcc cgacggcccg atgattcgcc tgttgccaag
     9781 ccgctcgcgc ggcccgggga caatgaccac ctcgatcctg ccgcccatga gcgcctccgc
     9841 gaagcaggtt ccctgtctgg gatttctcac cgtcgtcgaa cacgcggagt tgggcctgct
     9901 aggcggctat ttgctgctca acctggcggg ccggccgctg gaattccact gcaccgcgcc
     9961 ggtgaagccg aaccgggcgc aggagattct ctatgggccc acgctgcggc cgttcctgta
    10021 cggcgagcag attgggcaaa ccttgctggc ccgctcgaag ctgacgccgc tggttgcctg
    10081 caccgacagc gaaagcgtgc tggcggcccg cgactttaca gagactccgt tggtgctcgt
    10141 acgcgagggc gaggctgctg agctgaggtc gtttcgcctg ggccggaatc acgtgaccgc
    10201 gacgcctggc tatggcagcg atcagcaagc gatcctcgcc gcctggcccg cgcaggccga
    10261 tcacctcgac ttgtgcgagc cgttcgtgcg aattcgcgag gccttggaag aggcgcagaa
    10321 ggcggcgcgg gcagcgtagg cggcgacgga gcgttcggtg tttggtgttt tggactcggt
    10381 gatctgtgcc gccttcagct ctcaccatcg acctcgactt ggcggcccgg ccgctggcca
    10441 ctgacttcgc gctgcctctg ccagcgccga tcgtcgtccg ctcgcaaccg ctggggttca
    10501 gagccgaagc gccggccacg atcacggtga aaacgccgtc cagtcgcccc cgcgtcttca
    10561 gtttcacctt ccctgaagca gctccctctc tccgtctctc cctccctccc tctctccctc
    10621 ctgccgcacc aagaccaaag ctcgacggaa aaccgccgac gcgcatcaag ccccccggcg
    10681 atatcatcaa gctcgaagac cgtctgtatt acgtgcttca gccgccgctg gagacgctgc
    10741 ttgcctcgga cgcgctgcac tttccgttcg agccgtttcc gtatcagtat cagggagtgg
    10801 cgtttctcta tccgcggtat tcggccgtgc tggccgacga gatgggcctg ggcaagacga
    10861 tgcaggccat cacggcgatc cgcatgctgc tgcggtcggg cgagattcgc agcgtgctgc
    10921 tggtctgccc caagccgctg gtaaccaact ggcggcggga gttcaatctc tgggcggccg
    10981 agattccact gacgatcatc gaaggggacg cggccaagcg ccgctggcag tggactcagg
    11041 aagaggcccc ggtcaagatc gccaactacg agctcttgat gcgggacaag ggcgtgctcg
    11101 acgaatcgct ggcgttcgac ctggttgtgc tcgacgaggc ccagcggatc aaaaacacgg
    11161 gcagcacgac cagccagatc gcccgctcca tccgccgcac gcggaactgg gcgctgacgg
    11221 gcacgccaat cgaaaacacg cccgacgacc tggttggcat ctttgagttc ctctcgcccg
    11281 gctacctgcg aagcggcacc gcgccgcggc gaatggccga gctggtccgt gaacacatcc
    11341 tccgccgcac gaaggacatg gtgctgacgg atctgccgcc caagatgttc cgcgatgccg
    11401 agctcgatct ctcgcccgag cagcgggcca cctacgagct ggccgaaaat gaaggcgtga
    11461 tgcagctgaa cgcgatgggc gaccagatca cgatccagca cgtcttcgag ctggtcctgc
    11521 ggctgaagca gatctgcaat ttcgacccgg cgaccggcgc gagctcgaag ctggagcggc
    11581 tggaggccga tctggaggaa gtcgccgcca gcggcaaaaa ggcgatcgtc ttcagccagt
    11641 gggtcaagtg cctggagatt ttgaagcacc agctccgccg cttcggaccg ctcgaatatc
    11701 acggccaggt cccgagcaag cagcgcgacg gagtgctgaa gcaattccag gaggacccgt
    11761 ccaagcacgt gattttgatg agctacggcg cggggagcgt gggactcaac cttcaattct
    11821 gcgagtacgt tttcctgttc gaccgctggt ggaacccggc ggtcgaggac caggcgatca
    11881 accgggccca ccggattggc gcggccggcc cggtcacggt cacgcggatg ctcgccctgg
    11941 ccacgatcga ggagcggatc aaccaggtcc tcgagcacaa gcgcgagttg tttgcccaga
    12001 tcatcgggca gaattcggcg cctccctcgc tgggcctctc gcaggccgaa atctttggtc
    12061 tcttcaacct ccgcacgccc aagggcgtcc tcacgccggc tgcctagccg cggcccactg
    12121 gtcaagcgca ggcccgtgcc cgataattcg cggctcgatg cagttttcgc ggcacgtgag
    12181 gcgcgcaggg catgaaaatc cacgagtatc aggcgaagga catctttcgc aaaagcggtg
    12241 tcgcggtgct ccgcggcaag gtggcggcga gcgccgagga agcggccgcg gcattcaagg
    12301 agctgggggg gccattggcc gtcgtgaagg cgcagatcca cgcgggcggc cgcggcaagg
    12361 ggacgatcaa gagaaacccg cagcagcggg gcgtgcagct cgtccgctcg gccgaagaag
    12421 ccggcaaggt cgccaagaat ctgctgggcc aggagctggt gacgatccag accggccccg
    12481 aaggaaagac cgtcaaccgc gtgttcgtcg aggagggctg ccagatcgcc cgcgagctgt
    12541 acctggggat cgtgctcgac cgggcggtgg cgaaaccggt gctgatggtc tcgagcgaag
    12601 gcggagtcga gatcgaaaag gtcgcggccg aaacaccgca cctgatcttc aaggagcatt
    12661 tcgacccgca ggtgggcctg ctgccctacc aggtccgcaa gctgtgctac aagctcaagc
    12721 tgcaagggcc gaccgtcgcc agcgccgaga agttcatgcg ggcactttgc cggctgtacg
    12781 tacagctcga ttgctcgctg gtcgagatca acccgctggt ggtgactcag agcggcgagc
    12841 tgatcgcgct ggacggcaag ctgagcttcg acgacaacgc catgttccgg cacaaggacc
    12901 tggccgagct gcgggacctg tcggaggagg acccggccga ggtgcgggcc agcaacaccg
    12961 gcctgtcgta tgtcaagctc gacggcaaca tcggctgcct ggtaaacggg gcgggactgg
    13021 cgatgtccac gatggacatc atcaagctgc acggcggtga gccggcgaac ttcctcgacg
    13081 tcggcggcgg ggcgaacgag cagcaagtga ccgaggcctt tcggatcatc ctctcggacc
    13141 cgaacgtaaa gggcatcctg gtcaacattt tcggcggcat cgcccgctgc acgacgatcg
    13201 ccgcggccat cgtcgccgcc agcaaggcgg tcggcttcaa ggtgccgctg gtcgttcggc
    13261 tggaaggcac cgaggtcgag gagggcaaaa agatcctccg cgaaagcggc gtcaagctga
    13321 tcaccgccga cgatttgacc gacgcagcga agaagatcgt tgcggcagtc aaaggctgaa
    13381 cttgaccacc gactcaccga ccaccgatcc atgagcatcc tgatcaacaa atcgactcgc
    13441 gttatctgcc agggcatcac cggcaaggcg ggcgcgtttc ataccaaggg ttgcaaggac
    13501 tatggcacgc agatggtcgg cggagtgacg cccggcaagg gcggcgagac cgtcgaaggg
    13561 ctgcccgtct ttgacaccgt catcgaggcg gtcgagaaga ccggcgcaaa cgccacgatg
    13621 atcttcgtgc cggcggcgtt cacggccgat gcgatcctgg aggcgctcga cgcggggatc
    13681 aaagtcatct gcgcgatcac cgaaggcgtg ccggtgctcg acatggtccg cgtctacgag
    13741 ctggtccgca agagcgacgc cgtgctgatc gggccgaatt gcccgggcgt gatcacgccc
    13801 ggcgagtgca agatcggcat catgcccggc tacatccatc agccgggcaa ggtcggcatt
    13861 atgagccgca gcggcacgct gacctacgaa gcggtctggc agacgagcaa cctcaagctg
    13921 ggccaatcga cctgcatcgg cctcggtggc gatccaatcg tcggcacgaa ctacatcgac
    13981 ctcttcaagc ggtttcagaa cgatccacag accgaggcga tcctgatgat cggcgagatc
    14041 ggcggcacgg ctgaggaaga agctgccgcg tttgtgaaag agcacatcag caagccggtg
    14101 gccgcgttca tcgccggccg caccgccccg ccgggccgcc gcatgggcca cgccggcgcg
    14161 atcatcagcg gcggcaag
//
LOCUS       DASRJB010000635         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3850518, whole genome shotgun sequence.
ACCESSION   DASRJB010000635 DASRJB010000000
VERSION     DASRJB010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850518"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFB80_16885"
     CDS             complement(<1..1012)
                     /locus_tag="VFB80_16885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35508.1"
                     /translation="MSLAEAARPPATSRESPPSAAPARPARSPRVSPRGDMRCSIGDA
                     AAYSVMVGIGETYFAAFALALGTGETIAGLVATLPMLAGASLQLATPWALKRFHSFRT
                     WVVLCASLQAAALLLMPIAACLVGSAQVAWVFGAAAAYWAASQATGPAWNTWIEEIIP
                     KRVRANFFACRARISQSFTLLGFVLGGIALHVGKAQGWILATFTGIFLIGSACRFLSA
                     WFLNRQSEPSIGRYSTRHISFGEMLSRTQGDVGTSLVWYLLAVQTAVQISGPYFTPYM
                     LVHQQLDYFQYMVLIGIGFLGKVIALPLWGRVAHYAGARRLLWIGGTTIVPIAGLWIF
                     TGL"
     gene            1239..1958
                     /gene="ubiE"
                     /locus_tag="VFB80_16890"
     CDS             1239..1958
                     /gene="ubiE"
                     /locus_tag="VFB80_16890"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZN35509.1"
                     /translation="MTSGVIDKSGARVQRMFGEIAPHYDRMNHLLSLNIDRYWRSWTV
                     RKLQPTPGEPILDVCTGTGDLALAFHRATGGKSEIVAADFCPQMLEIGRRKQARAKIG
                     SELTFVEADAQNLPFEDNRFAIVAVAFGLRNVTDTNRGLAEMTRVCREGGQVAVLEFS
                     TPRWQPFRGCYLLYFHHILPRVGQFFARNKSEAYEYLPATVAQFPDGEALAERMRGVG
                     LKNIKYWPLTGGIATLYVGRK"
     gene            complement(1968..>2218)
                     /locus_tag="VFB80_16895"
     CDS             complement(1968..>2218)
                     /locus_tag="VFB80_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35510.1"
                     /translation="EYRTHIPGNYDFYDFKGRDISTLVAPRIESKSEFLRLVSNPRIA
                     IAAWDREAIGFSVHLDCTWSALGVEIRITQGKIDRVSV"
BASE COUNT          447 a          730 c          698 g          343 t
ORIGIN      
        1 acagcccggt gaatatccac agcccggcga tcggcacgat cgtcgtgccg ccgatccaca
       61 ggagccgtcg cgcgccggca tagtgggcga cgcggcccca caacggcagg gcgatcacct
      121 tgcccagaaa gccgatgccg atcagcacca tgtactggaa atagtcgagc tgctggtgga
      181 ccagcatgta gggcgtgaag tacggcccgg aaatctgcac cgcggtctgc acggcgagca
      241 gataccagac gagcgacgtg cccacgtcgc cctgcgtccg cgaaagcatc tcgccaaacg
      301 aaatgtgccg cgtgctatag cggccgatcg acggctccga ctgccggttg aggaaccagg
      361 ccgagaggaa ccggcaggcc gagccgatca ggaagatgcc cgtgaacgtc gccaggatcc
      421 agccctgggc tttgccgaca tgcagcgcga ttccgccgag gacgaagccc agcagcgtga
      481 acgactggct gatgcgggcg cggcaggcga agaagttcgc ccgcacgcgc ttggggatga
      541 tctcttcgat ccacgtgttc caggccgggc cggtcgcctg gctggccgcc caataggccg
      601 ccgccgcgcc gaacacccag gccacttgcg ccgagccgac caggcaggcg gcaatcggca
      661 tcagcagcaa agccgccgcc tgcaagctcg cgcagagcac gacccaggtg cggaacgagt
      721 ggaagcgctt gagcgcccag ggcgtcgcca gctgcagcga ggcccccgcc agcatcggca
      781 gcgtcgccac caggccggcg atcgtctcgc cggtccccag cgccagcgcg aacgccgcga
      841 agtaggtctc gccgatcccg accatcacgc tgtacgcggc cgcgtcgccg atgctgcacc
      901 gcatgtcgcc acgcggcgaa acccgcgggc tgcgcgcagg gcgagccggc gcggctgatg
      961 ggggcgactc ccggctggta gcgggcggcc gggcggcctc ggcaagcgac atggaaggct
     1021 cagttggggc gggcagttac aatatcgtac taacggcggt caataacgtg agaggggaca
     1081 gctaattacc ggttagcgtt cgcagcgcca acggcgaatc cggccgcccg ccaacgcagc
     1141 cgtaactcat tgtgcagcaa cgacttccgc acggtcgcgg cggctgctcg actcgacatc
     1201 gaaccagcag ccgcggccgt tcaactaaac tctatgccat gacgagcggc gtgatagaca
     1261 aatcgggggc gcgggtgcag cggatgttcg gcgaaatcgc gccgcactac gaccgcatga
     1321 accacctgct gtccctcaac atcgaccggt attggcggtc gtggacggtc cgcaagcttc
     1381 agccgacgcc cggcgagccg atcctggatg tctgcacagg cacgggagac ttggcactgg
     1441 cgtttcatcg cgcgacgggc ggtaaatccg aaatcgtcgc ggccgacttt tgtccgcaga
     1501 tgctcgagat cggccgccgc aagcaggccc gcgcgaaaat cggcagcgag ctgaccttcg
     1561 tggaagccga cgcgcagaac ctgcccttcg aagacaaccg cttcgcgatc gtcgcagtcg
     1621 ccttcggcct gaggaacgtg accgacacca accgcggcct ggccgaaatg acgcgcgtct
     1681 gccgcgaggg cgggcaagtc gccgtgctgg agttcagcac gccccgctgg cagccgtttc
     1741 gcggctgcta cctgctctat ttccaccaca tcttgccgcg agtcggccag ttcttcgccc
     1801 gcaacaaatc cgaggcctac gaatatctcc ccgcgaccgt cgcccagttc cccgacggcg
     1861 aggccctcgc cgagcggatg cgcggcgtcg gcctcaagaa catcaagtac tggccgctga
     1921 ccggtggaat tgcgacgctg tatgtgggac ggaagtagcc actattatca gacggacacc
     1981 cgatcaattt tcccctgcgt gattcgaatc tcgacaccaa gagcgctcca cgtgcaatcg
     2041 aggtgaactg agaatccgat tgcctctcga tcccatgctg cgattgcaat tcgcggattc
     2101 gaaaccaaac ggagaaactc tgattttgat tcaatacgtg gggcaactag tgtcgagata
     2161 tcgcggccct tgaaatcata aaagtcataa tttcctggaa tgtgagttcg gtattcct
//
LOCUS       DASRJB010000636         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3850978, whole genome shotgun sequence.
ACCESSION   DASRJB010000636 DASRJB010000000
VERSION     DASRJB010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850978"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFB80_16900"
     CDS             <1..343
                     /locus_tag="VFB80_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN35511.1"
                     /translation="GAFQMTGMELSTIVRRGGCPIVIVLDNGGYGTERFLHHGDWKYN
                     DIHPWKYHKLPEILGGGTGYEVFTEGQLDAALGQAWNDRRGFSLIQAHLPKLDASDAL
                     RRLTERLSQRV"
     gene            complement(363..962)
                     /locus_tag="VFB80_16905"
     CDS             complement(363..962)
                     /locus_tag="VFB80_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HZN35512.1"
                     /translation="MAKRDNHYEAAFEAWLRAARVPFVAVNEARRPDAGRRSLKNIDF
                     IVSPAVQPESWLVDIKGRQFPTAGRQFWRNWSTADELASLASWETLLGPQFCGLLVFA
                     YQVVGDRAPLAADELFFFRGRLYGFLGVRLDHYSSFARPLSTRWNTVTLPVPQFRALA
                     RPVREFFGVPLSPPETAVVRGSISDFKFQISDCDSKSEI"
     gene            1127..>2652
                     /gene="mutS"
                     /locus_tag="VFB80_16910"
     CDS             1127..>2652
                     /gene="mutS"
                     /locus_tag="VFB80_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZN35513.1"
                     /translation="MAATPMMQQYQDAKAACGEALLLFRMGDFYELFYDDAKTAARVL
                     GLTLTSRDKGENPVPMAGFPYHQLESYLGKLIAAGLRAAVCEQVEDPKLAKGLVKREL
                     TRVVSPGTLTDDALLDPRQSNYLAAVALPAHQDNGDSRAGIAWAELSTGRFLAASMPL
                     ARLADELARIGPAECLLAEEAELPAAVAGRTMLTRRPAWAFNLQTATATLQQQFQTAT
                     LGGFGFDERDGPALAAAGAVLDYLRETQKTSLAHFDRLLPYRAGQALEIDEATRRSLE
                     LTRTIRSGSREGSLLAVIDQTVTPLGARLLADWLANPLTQASAIDARLDAVAHLAADE
                     GLRAGLREKLRGVYDLERLLARVATGRASPRDLSFVARTLAGLPKLKELLASGGRQPP
                     GGAAAATGVATLSGCSRTPLAGATAGLLTQLEAAIDPCPELAAQLASALVDDCPLAAR
                     EGGIFRGGYHGPLDSLRDLAAGGKQWIARYQAEEARKTGIANLKVAYNKVFGYYIEIT
                     "
BASE COUNT          456 a          910 c          906 g          380 t
ORIGIN      
        1 cggggcgttc cagatgaccg ggatggagct ctccacaatc gtccgccgcg gcggctgccc
       61 gatcgtgatc gtgctcgaca acggcggcta cggcaccgag cggttcctgc accacggcga
      121 ctggaagtac aacgacattc acccctggaa gtaccacaag ctgcccgaaa tcctgggcgg
      181 cggcaccggc tacgaggtct tcacggaagg ccagctcgac gccgccctgg gccaggcctg
      241 gaacgaccgc cgcggcttca gcctgattca ggcccacctt cccaagctcg acgccagcga
      301 cgctctgcgg cggctcaccg agcggttgag ccaacgcgtc tagcaatttc agatttagga
      361 cttcagattt cagatttgga atcgcaatct gaaatctgaa atttgaaatc tgaaatcgac
      421 ccgcggacga ccgcagtctc aggtggagat aaggggacgc cgaaaaactc gcgcactggc
      481 cgggccaggg cgcggaattg cggcacgggc agcgtgaccg tgttccaccg cgtcgagagc
      541 gggcgggcga agctgctgta gtggtccagc cgcacgccga gaaagccgta cagacggccg
      601 cgaaagaaga acagctcgtc cgccgccaag ggcgcgcggt cccccacgac ctggtaagca
      661 aagaccagga ggccgcagaa ctgcggcccc agcagcgttt cccagctggc caggctggcc
      721 agctcgtcgg ccgtggacca gttccgccag aactggcgcc cggccgtcgg gaactggcgg
      781 cccttgatgt ccacgagcca gctttccggc tgcaccgcgg gcgaaacaat gaagtcgatg
      841 ttcttcagcg agcgccggcc cgcgtcgggg cggcgggcct cgttcaccgc cacaaacggc
      901 acgcgcgccg cccgcagcca ggcctcgaac gccgcctcgt aatggttgtc gcgcttggcc
      961 atgtttcgcg ctcgcggcgc tcattagatc aaagtcgagc ggttggagcc aagagggagt
     1021 gacggagaga aggagagaca gagcgacgga gggacggagg gactgggtct gtcgccccct
     1081 ctctccgtcc ttcccgctct ctgccgcaca cgctaaattc ccccgcatgg ccgcgacccc
     1141 gatgatgcag cagtatcagg acgccaaggc ggcctgcggc gaggccttgc tgctgttccg
     1201 gatgggcgat ttttacgagc tgttctacga cgacgccaag actgcggcgc gggtgctggg
     1261 gctgacgctc actagccggg acaagggtga aaaccccgtg ccgatggccg gctttcccta
     1321 ccaccagctc gagagctacc tgggcaagct gatcgccgcg ggactgcggg ccgcggtctg
     1381 cgagcaggtc gaggacccca agctggccaa gggactcgtc aagcgcgagc tgacgcgggt
     1441 cgtctcgccg ggcacgctga ccgacgacgc gctgctcgac ccgcggcaga gcaactacct
     1501 ggcggccgtg gccctgccgg cccatcagga caacggcgac tcccgcgcgg ggatcgcctg
     1561 ggccgagctt tcgaccgggc ggttcctggc ggcgagcatg ccgctcgcac ggttggcgga
     1621 cgagctggcc cgcatcggcc cggccgaatg cctgctggca gaagaggccg agctgccggc
     1681 ggcggtggcc ggccgcacca tgctcacccg gcggccggcg tgggcgttca acctgcagac
     1741 ggcgaccgcc accctgcagc agcagttcca gacggcgacg ttgggcggct ttggcttcga
     1801 cgagcgcgac ggcccggcgc tcgccgccgc aggcgcggtc ctcgactatc tccgcgaaac
     1861 acaaaagacc agcctggccc atttcgatcg cctgttgccc taccgcgcgg gccaggcgct
     1921 ggaaatcgac gaagccaccc gccgcagcct ggagctcacc cggaccatcc gcagtggcag
     1981 ccgcgagggc tcgctgctgg ccgtcatcga ccagacggtg acgccgctgg gggcgcggct
     2041 cttggccgac tggctggcga atccactcac ccaggccagc gcgatcgacg cccgtctgga
     2101 cgccgttgca catctggccg cggacgaagg gttgcgggct gggctgcgcg agaaattacg
     2161 cggcgtctac gacctggagc gcttgctggc ccgcgtggcc accggccggg ccagccctcg
     2221 ggacctgtcg ttcgtggcgc ggacgctggc cgggctgccg aaactgaagg agctcctggc
     2281 gagcgggggg cgtcagcctc ccggtggcgc ggcagcggca accggcgttg cgacgttatc
     2341 ggggtgctca cgcaccccgc tcgccggcgc gactgccggc ttgctgaccc agctggaagc
     2401 cgcgatcgat ccctgcccgg agctggcggc gcaactggcg tcggcgctgg tggacgactg
     2461 cccgctggcg gcccgcgagg ggggcatctt ccgcggcggc tatcacggcc cgctcgacag
     2521 cctccgcgac ctggcggccg gcggcaagca atggatcgcc cgctaccagg ccgaagaggc
     2581 gcggaagacc ggcatcgcca atttgaaggt cgcctacaac aaggtcttcg gctactacat
     2641 cgagatcacc aa
//
LOCUS       DASRJB010000637         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_471275, whole genome shotgun sequence.
ACCESSION   DASRJB010000637 DASRJB010000000
VERSION     DASRJB010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471275"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFB80_16915"
     CDS             complement(<1..733)
                     /locus_tag="VFB80_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN35514.1"
                     /translation="MASVLVTGGAGFIGSHLTEALLARGDHVTVIDDESTGRFENLAV
                     VKGNPGLTLVRGSVGDASLLRQHLDGVGEVYHLAAAVGVALIAREPLQTIERNIYPTQ
                     LLLDELCRRHAAGQLLKFFLASTSEVYGKNPKPTWTEEDDLVFGATTRPRWSYGASKA
                     IDEFLTLACARQFGLAVVIGRFFNVVGPRQTGAYGMVLPRFVEAALAGQPLVVHDDGR
                     QIRCFAHVGDVVAAVLTLMQSPAALG"
     gene            989..4198
                     /locus_tag="VFB80_16920"
     CDS             989..4198
                     /locus_tag="VFB80_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35515.1"
                     /translation="MNLIFRTTSLGLAIVLIVSAGWARGQEKSAESSPAAVQLYRDAA
                     NFQNNGAFDLAAEEWQKFLKNHSKDPLAAKAQHYLGVCQLQLKQLEAAAASFEKVLKD
                     YPKFELRDEAFLNLGSCQYSLAAGGKIELYAKAAGTFQTLLIDFPKSKHIEDALYYQG
                     ESLYGQGKKQEAAAAYERLLKDFAKSKRRADTLYALGVAQEELGQHAAAGKTYDVYLA
                     EFAGAPLASEVRMRKAETILQAGDAAAAAKIFAEVAAVKDFAARDHALSRQAFCLARL
                     DKFAEAGALYARLATENPQSPLAADAALSAGRCYYRADKPAEAQSWFEKAVAKNDAGA
                     AEAAHWLCRILIKGGKAVEAADLAGQQLAAAKDAPFAANLALDQADALYEIPAKRAEA
                     LALYAKFAADRPQHELAPQALYNAAFAALDLKQYDEALRRTAAFLSAYPQDKLVPDVK
                     YVAAESNVQLKKYDEAARQFRELTEKHAGHADSDAWRVRTGLAAYLDKKYDAAIAALA
                     PITGQLKSPDHQAEAQFLIGASQFYSDKFAQAATALSASLAANPKWKQADETLLLLAR
                     AESKLGKPADAKAKLAKLLADIPDSSLADQAHYRLGELAYAGDDFPAAIAAYQTVATR
                     YAASPFAPFALYGQAWSQLKSQDFKAGADSFTSLISKFPENQLAADARLGRAICRRQS
                     GDPQGAIADIDDYLKTNPDQAHQSDARYERGLAQVAANDFAGAAATLDALLKADPKYA
                     GADKVLYEVGWALKSQNKHAEAAAAFARIVAEHANSPLAAEAAFHVGEDQYEKKQYAE
                     AAKSYAAAKAKNPGGELGEKAAYKLGWAHFQQQKYADALAQFGEQLAAFPQGMLAADA
                     VFMKAECLFRQEKFQEAWPAYQAAIKTKASSDQIAVLTLLHGAQSAAQLKQWDESAQL
                     LAQIPAKFAQSPNVPEALYELGWAQQNLGQTAEAIKSYEAAATQSREHVGARARFMIG
                     ELLFSQKKHDEAIKEFQRAMFGYGGEQASGETKNWQAKSGYEAGRCAEVQIMSAADAA
                     AKQKHLADARRFYTFVTEKHASHELAAEAAKRLTALSKL"
BASE COUNT          827 a         1439 c         1424 g          653 t
ORIGIN      
        1 ggcccagcgc ggcgggcgat tgcatcagcg tcagcaccgc cgcgacgacg tcgcccacgt
       61 gcgcgaagca gcggatctgc cggccgtcgt cgtgtacgac cagcggctgg ccggccagcg
      121 ctgcctcgac gaaccgcggc agcaccatgc cgtaggcgcc cgtttgccgc gggccgacaa
      181 cgttaaagaa ccggccgatt acgacggcca gcccgaactg ccgggcacag gcgagcgtca
      241 ggaactcatc gatcgccttg cttgcgccgt acgaccaccg cggccgcgtc gttgcgccga
      301 agaccaggtc gtcctcctcg gtccaggtcg gcttgggatt cttgccatag acctcgctgg
      361 tgctggcgag gaagaatttc agcagctgcc ccgcggcgtg ccggcggcag agctcgtcca
      421 ggaggagctg cgtcggatag atgttccgct cgatcgtctg cagcggttcg cgggcaatca
      481 gcgccacgcc gaccgctgcc gccaggtgat agacctcgcc gacgccatcc aagtgctggc
      541 ggagcaggct ggcatcgccg acggaaccgc ggacgagcgt cagccccgga ttgcccttta
      601 ccacggcgag gttttcgaac cggccggtcg attcgtcgtc gatgaccgtg acgtggtcgc
      661 cgcgggcgag cagggcctcg gtcaaatgcg agccgatgaa acccgcgccg ccagttacca
      721 ggacgctcgc cacgactcgc tatctgctcc ttgttcgctg gaaacgacgg ccctgagcgg
      781 cctggtagca gccgttgtaa cgtcccgccg ccgcggcgac cacctaatgg gcagcgcaaa
      841 gcatcctatt cctttaccct catcgcctac gcatataaac tggaagctgc ttaggggttt
      901 ggcggtttgt ggtgaggggc acgccgtaag tgcttgcccg acaaagacct gcggctgcaa
      961 ggcgacccgt tcacgggaac gacggcagat gaacttgata ttcagaacga cttcgctcgg
     1021 cttggccatc gtcttgatcg tttccgccgg ttgggcccgc ggccaagaga aatccgccga
     1081 atcttcgccc gcggcggtgc agctgtatcg cgacgcggcg aactttcaaa acaacggggc
     1141 cttcgacctg gcggccgagg aatggcagaa gtttctcaag aatcattcca aagatccgct
     1201 ggcggcaaag gcccagcact acctgggcgt ctgccagctc cagctcaagc agctcgaggc
     1261 cgccgcggct tcattcgaga aggtgctgaa ggactatccg aagttcgagc tgcgcgacga
     1321 agcgtttctc aatctgggct cctgccagta ttcgctcgcc gccggcggca agatcgagct
     1381 ctacgccaaa gcggccggca cgtttcaaac gctgctgatc gactttccca agagcaagca
     1441 catcgaggac gcgctctact atcagggcga gtcgctctat ggccagggca agaagcagga
     1501 ggccgccgcg gcctacgagc ggctgctgaa ggattttgct aagtccaagc ggcgggccga
     1561 tacgctctat gccctgggcg tcgcgcagga ggagctgggg cagcacgccg cggcgggcaa
     1621 gacgtacgac gtctacctgg cagagttcgc cggagcgccg ctggccagcg aagtgcggat
     1681 gcgcaaggcc gagacgatcc tccaggcggg cgacgccgcc gcggccgcga agatttttgc
     1741 agaggttgcc gcggtgaagg atttcgccgc gagggaccat gccctgtcgc ggcaagcgtt
     1801 ctgcctggcc aggctcgaca agttcgccga ggcaggcgcg ctctacgcgc gcctcgcaac
     1861 ggagaatccc cagtcaccgc tggctgccga tgcggcgctg tcggcgggac ggtgctacta
     1921 ccgggcggac aagccggccg aggcgcagag ctggttcgag aaggccgtgg cgaagaacga
     1981 cgccggcgcg gcagaggcgg cgcactggct ctgccggatt ctgatcaagg gcggcaaagc
     2041 cgtcgaggcg gcggacctgg ccggccagca gctggccgcg gcgaaggacg ctccgttcgc
     2101 ggccaatctg gccttggacc aggccgatgc cctgtatgag attccagcca agcgcgcgga
     2161 ggcgctggcg ctgtacgcca aatttgccgc cgatcggcct cagcacgagc tcgccccgca
     2221 ggcgctctac aacgcggcct tcgcggcgct ggacctcaag cagtacgacg aggcgctgcg
     2281 gcggaccgcg gcgttcctct cggcctatcc gcaggacaag cttgttcccg acgtgaagta
     2341 cgtcgcggcc gagtcgaacg tacagctaaa gaagtacgac gaggccgcgc gccagttccg
     2401 cgagctgacc gagaagcacg ccggacacgc cgacagcgac gcctggcgag tccggacggg
     2461 gctggccgct tacctcgaca agaaatacga cgccgcgatt gcggccctcg cgccgatcac
     2521 cggccagctc aagtcgcctg atcaccaggc cgaagcgcag ttcctgattg gcgcgagcca
     2581 gttctactcc gacaagttcg cccaggcggc aacggccctg agtgcatcgc tggcggccaa
     2641 tcccaagtgg aagcaggccg acgaaacgct gctgctgctc gcccgcgccg agagcaagct
     2701 cggcaagccg gccgacgcca aggcgaagct cgcgaagctg ctggccgaca tccccgatag
     2761 cagcctcgcc gaccaggcgc actaccgcct gggcgagctg gcctatgccg gcgacgactt
     2821 tcccgcggcg atcgccgctt atcagacggt cgccaccagg tacgccgcgt cgccgtttgc
     2881 tccgtttgcc ttgtacggcc aggcctggtc gcagctcaag agccaggact tcaaagcggg
     2941 cgcggactca ttcacgtcgt tgatctcgaa gtttccggag aaccagcttg ccgcagatgc
     3001 ccgcctgggc cgcgccatct gccgccggca gtcgggcgat ccgcagggag cgatcgccga
     3061 catcgacgac tatttgaaga ccaacccgga ccaggcgcac cagagcgacg cccgctacga
     3121 gcgcgggctg gcgcaagtcg ccgccaacga tttcgccggc gccgccgcga ctctcgatgc
     3181 gctgctcaag gccgatccca agtacgccgg ggccgacaag gtgctctacg aagtcggctg
     3241 ggccctcaag tcgcagaaca agcacgccga ggccgcagca gccttcgcgc ggatcgtcgc
     3301 cgagcacgcc aatagcccgc tcgcggctga agcggcgttc cacgtcggcg aggatcagta
     3361 cgagaaaaag caatatgccg aggcggcgaa atcgtacgcg gcggccaaag ccaagaatcc
     3421 cggcggcgaa ttgggagaga aggccgccta caagctgggc tgggcccatt tccagcagca
     3481 gaagtatgcc gacgccctgg cgcagttcgg cgagcagctg gcggcgtttc cccagggaat
     3541 gctggccgcc gacgccgtgt tcatgaaggc cgagtgcctg tttcgccagg agaaattcca
     3601 ggaagcctgg cccgcttacc aggccgcaat aaagaccaag gccagcagcg accaaatcgc
     3661 cgtgctgacg ctgctgcacg gcgctcagag tgcggcgcag ctcaagcaat gggacgagag
     3721 cgcgcagttg ctggcccaaa ttcccgcgaa gtttgcgcaa tcgccgaatg tgcccgaagc
     3781 cttgtacgag ctgggctggg cgcagcagaa cctcggccag acagccgaag caatcaagag
     3841 ctacgaagcc gcggccacgc agtcccgcga gcacgtcggc gcgcgggccc gcttcatgat
     3901 cggcgagctg ctcttttcgc agaagaagca cgacgaggcg atcaaggagt ttcagcgggc
     3961 gatgttcggt tacggcggcg agcaagccag cggcgagacg aagaactggc aggccaagag
     4021 cggctatgaa gccggccgct gcgccgaagt gcagatcatg tctgcggccg acgccgccgc
     4081 gaagcagaag cacctggccg acgcccggcg gttttacacg ttcgtcaccg agaagcacgc
     4141 cagccacgaa ctggccgccg aagcggccaa gcggctgacg gcgctcagca agttgtaacc
     4201 tcctttcgag aacgaggcat gttcctgaac tccgacaatc gcgcggcctg gtacgtacgc
     4261 ctgggcctgt atctggccgc cggcatcgcg gccattgctt tcagctggtc gctgcccaag
     4321 atgcccgctg ctcacgctca gac
//
LOCUS       DASRJB010000638         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_471503, whole genome shotgun sequence.
ACCESSION   DASRJB010000638 DASRJB010000000
VERSION     DASRJB010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471503"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFB80_16925"
     CDS             complement(<1..744)
                     /locus_tag="VFB80_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZN35516.1"
                     /translation="MFSTRTNSRLPRLEWLESRQLLAGNLGSITFEENVLTILGTSKN
                     DRVSVTLVATEQGEFLRVALNKLEPLWLTPADVTKINIDVGNGNDRVEIGANVAIDAW
                     ISGGKGNDDLRAGGGNDQVFGDDGNDKVSGGDGNDLVDAGAGQDAANGGMGDDHVLGG
                     AGHDRLTGGWGNDILEGGAGKDHLAGNDGDDQLLGGDADDKLTGGLGNDLLDGGGGHD
                     HLSGEAGDDEAHGGAGKDQIKGGWGNDQLW"
     gene            complement(908..>2268)
                     /locus_tag="VFB80_16930"
     CDS             complement(908..>2268)
                     /locus_tag="VFB80_16930"
                     /inference="COORDINATES: protein motif:HMM:NF020322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN35517.1"
                     /translation="EFLSRQLASNQFFSGGLILMVSGAALALARHIPGKIWAWLKDRC
                     LTEIDVPDREAAFEWLDKWLAAHVYSQRRARRLTVRVQPLDHRQRAADPAGDHRPRLL
                     FAPAPGEHWLWYRGRLVILNRQRADTEKNAQGQAPISIRETFSITIVSRNRQLAWQLL
                     EDAREHALPRNDRRLTIYHVTYGSWSEQMQRLPRPPESVVLRAGQMEDLIADARRFLD
                     GREWYVQRGIPYRRGYLLHGPPGSGKSSAVVGIATALGMDVAVLNLSSFSLDDNELAD
                     RLAAVPPNAIVLIEDIDCAFVARKSSEEKTSRITFSGLLNAIDGVAAGEGRLLFATTN
                     HPERLDPALIRPGRIDRRVEIGHADREQLARIFRRFFPGANPDLAEQFAAALPERQLA
                     MSGVQAYLIQHSSSAEQALAHVGSLYAVSAVPLGCALRAAADDPSGGTWRLRELEAFT
                     RG"
BASE COUNT          340 a          765 c          773 g          390 t
ORIGIN      
        1 ccacagctgg tcgttgcccc agccgccctt gatctggtcc ttgccagcgc cgccgtgggc
       61 ctcgtcgtct ccggcttcgc cgctcagatg gtcgtggccg ccgccgccgt cgagcaggtc
      121 gttgcccaga ccgccggtca gcttgtcgtc ggcgtcgccg ccgagcagct ggtcgtcgcc
      181 gtcgttgccg gcgaggtggt ccttgcccgc gccgccttcc aggatgtcgt tgccccagcc
      241 gccggtcagc ctgtcgtggc ccgcgccgcc gaggacgtga tcgtcaccca tgccgccgtt
      301 ggccgcatcc tgcccagcgc cggcgtcaac gaggtcgttg ccgtcgccgc cgctgacctt
      361 gtcgttgccg tcgtcgccaa acacctggtc gttgccgccg ccggcgcgca ggtcatcgtt
      421 tcccttgccg ccgctgatcc aggcgtcgat cgctacattc gcgccgatct cgacccggtc
      481 gttgccgttg ccgacgtcga tgttgatttt cgtgacgtcg gcaggcgtca gccagagtgg
      541 ctcgagcttg ttgagcgcca cgcgcaggaa ttcgccctgc tcggtcgcca ccagcgttac
      601 cgacacgcgg tcgttcttgg acgtgccgag gatcgtcagc acgttctcct caaaggtaat
      661 cgagcccagg ttgcccgcca gcagctgacg agattccaac cattccaagc gcggcagacg
      721 actattcgta cgagtggaga acatagcgcc ctcctgaagt tgacaagtgg ctggcctcgc
      781 gtggaaaagg gctcgtggct ctcagcaccc cgcgagctcg cgaaaggcca tcgaaaccct
      841 aggtcggcag tgggctttgt caaacggagt gggcgctttc cgcttggcgc ggcactttgc
      901 cggggtttta gccccgcgta aacgcttcca actcccgcaa gcgccaggtg ccgccgctag
      961 ggtcgtcggc agcggcgcga agggcgcaac ccagaggtac ggcgctgacg gcataaagcg
     1021 aaccgacgtg cgctagcgct tgctcagccg atgaggaatg ctgaatcaaa tacgcctgca
     1081 caccggacat cgcgagttgc cgctcgggca gggcggcagc gaactgctcg gccaggtccg
     1141 gattggcgcc tggaaagaac cgccggaaga tccgtgccaa ctgctcgcga tcggcatggc
     1201 cgatttcaac gcggcgatcg atgcggccgg ggcggatcag cgcgggatcg agccgctccg
     1261 gatggttggt cgtggcgaag agcagccgcc cctcgcccgc ggcgacgccg tcgatcgcgt
     1321 tcaagaggcc gctgaaggtg atccggctgg tcttctcctc gctgctcttg cgggccacga
     1381 acgcgcagtc gatgtcctcg atcagcacga tcgcattggg cggcacggcc gccagccggt
     1441 cggccagctc gttgtcgtcg agcgagaagc tgctcaggtt tagcacggcg acatccatgc
     1501 ccagggccgt ggcgatgccc acgaccgccg agctcttgcc gctgccgggc ggcccgtgca
     1561 gcaggtagcc gcggcgatag ggtatgccgc gctggacgta ccattctcgc ccgtccagga
     1621 agcggcgggc gtcggcgatc aggtcttcca tctgcccggc ccgcagcaca accgattccg
     1681 gcggccgcgg cagccgctgc atctgctcgc tccacgagcc gtaggtcacg tgatagatgg
     1741 tcagccggcg gtcgtttcgc ggcagggcgt gctcgcgagc gtcttcgagc agctgccagg
     1801 ccagttggcg gttgcggctg acgatggtga tgctgaaggt ctcgcggatc gagatggggg
     1861 cctgcccctg ggcgttcttc tcggtgtcgg cccgctggcg gttgaggatc accagccggc
     1921 cgcggtacca gagccagtgc tcgccggggg ccggggcgaa gagcagccgc gggcggtggt
     1981 cgccggccgg atcggcggcc cgctggcgat ggtccagcgg ctggacgcgg acggtaaggc
     2041 ggcgggcccg gcgctgcgag tagacgtggg cggcgagcca cttgtcgagc cactcgaacg
     2101 ccgcctcgcg gtctggcacg tcgatttccg tcaggcagcg gtccttcagc caggcccaaa
     2161 tcttccccgg aatatgccgg gcgagagcca gcgccgcgcc cgaaaccata agaatcaagc
     2221 cgccggagaa aaactggttg ctcgccagtt ggcggctgag aaactccc
//
LOCUS       DASRJB010000639         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4135286, whole genome shotgun sequence.
ACCESSION   DASRJB010000639 DASRJB010000000
VERSION     DASRJB010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135286"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFB80_16935"
     CDS             <1..712
                     /locus_tag="VFB80_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN35518.1"
                     /translation="HELRNPLAPIRSAVDVLRLCDGRPDDIAWARDVIDRQVNHMIRL
                     VDDLLDISRITLGKIRLKLEPLQAAELVAASVELSRPLIEKSGHQLTLDLPELPLALM
                     GDRARLTQVIANLLNNAAKYTPKGGSIFVSLKEEAGEAVFCVRDTGIGIPRGMLDRVF
                     DMFLQIDNSLDRAQGGLGIGLTLVRELVHLHRGIVTVNSDGPGQGSEFTVRIPCLAAG
                     LQPEDAVTVTAEMPALVK"
     gene            complement(705..1202)
                     /locus_tag="VFB80_16940"
     CDS             complement(705..1202)
                     /locus_tag="VFB80_16940"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZN35519.1"
                     /translation="MVDRPAIQTNGRDKTDRDYTAPRAVAHNLSELMHDALTLGDLQL
                     RLAWLDARLLSKELVYPGVLLLVGVTVVLSCVPIALATIALALDEATRLTAAQAFGFT
                     LAGGLVVGAVTALAAVLWIRRGVHPFRRSIAECDANVQWIKKVLQQNIGAARKSRPID
                     ESAVI"
     gene            complement(1218..1658)
                     /locus_tag="VFB80_16945"
     CDS             complement(1218..1658)
                     /locus_tag="VFB80_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35520.1"
                     /translation="MPSDSADEIQQQMRQVRRELREDVEEIVENAQVLTDWRHYVRRY
                     PVVCLGAAAAAGYLLVPARRDFRPDPAAIADALQEQALKLTPPAPRKSLFSSVLGLVG
                     PLVWQAAMTLGKQQLAHYLNGHQAADAGETHPPADPERPRPHVH"
     gene            complement(1716..1886)
                     /locus_tag="VFB80_16950"
     CDS             complement(1716..1886)
                     /locus_tag="VFB80_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZN35521.1"
                     /translation="MLQWALTFLVLALIAAVFGFGGIAASFAWIGQVLFVLFLILFVV
                     SLITGGVRSPRV"
     gene            2037..3521
                     /locus_tag="VFB80_16955"
     CDS             2037..3521
                     /locus_tag="VFB80_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN35522.1"
                     /translation="MAGVVVIDDDLSVVVLIRAACKQDNVAVHAAETPEEGLELLKRV
                     QPDVLLLDVVLPGVSGLELFERIRRIDARVPVIFITASGESETAIEAMKLGALDFLLK
                     PLDVTRVRELIGQALRIRQLMATPVELPRPAAGGDSQLIGRSPQMLEVYKAIGRVAPQ
                     SVTVLICGESGTGKELIARAIYQHGDRKNKPFLAVNCAALSETLLESELFGHEKGSFT
                     GADHRRIGKFEQCNGGTIFLDEVGDMPPLMQAKLLRLLQEQRFERVGGNETIQTDVRI
                     ITATNQDLEAMSAAGTFRADLYYRLNGFTIRLPPLRDRGDDLLLLVEAFLSRYSQELD
                     KPLTRVSPDAMKILLEYSWPGNIRELQNILRKSLLNLSGPILLPEFLPEEVRHPSQAS
                     GGESSAASGNGGLPHDLAPFLAERMAAGSENIYAEAINYLDRFLVTRILQSTSGNQSK
                     AAKLLGITRGSLRNKVQALDIKIGHVVKGPEDEDAADLAAEAGG"
     gene            complement(3518..3673)
                     /locus_tag="VFB80_16960"
     CDS             complement(3518..3673)
                     /locus_tag="VFB80_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZN35523.1"
                     /translation="MLGTILLIILILLLLGAIPAWPHSRAWGYGPSGGLGLIVVIVLI
                     LVLLGRI"
     gene            complement(3762..4409)
                     /locus_tag="VFB80_16965"
     CDS             complement(3762..4409)
                     /locus_tag="VFB80_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN35524.1"
                     /translation="MARHRGLHENLETVVHELRNTLAPLTASCQLIVLDPGNSEQVRE
                     LAAVMSRQLDELRQLVDDVLETSRNSADGRAADVADPEPVGPAAAAEELPAFRLLVVD
                     DEPSATHLLARVLEKLGQQVHVAQSGDEALALLPTLAADAVISDIGMPGLSGYELAAR
                     IRKLPLTRQPLLVAVTGYGQESDRRAALGAGFDRHLKKPVGLPELRGIIEALARR"
BASE COUNT          782 a         1515 c         1497 g          725 t
ORIGIN      
        1 ccacgagctg cgcaatcccc tggccccgat ccgcagcgcg gtcgatgtgc tgcggctgtg
       61 cgacggcagg ccggacgaca tcgcctgggc ccgcgacgtc atcgaccggc aggtgaacca
      121 catgatccgc ctggtggacg acctgctcga catctcgcga atcacgctgg gcaagatccg
      181 cctcaagctg gagccgctgc aggccgcgga gctggtggcg gccagcgtcg agctcagccg
      241 gccgctgatc gagaagtcgg ggcatcagct gacgctggac ctgcccgagc tgccgctggc
      301 gctgatgggc gaccgggccc gcctgacgca ggtcatcgcc aacctgctca acaacgcagc
      361 caagtacacg cccaagggcg gctcgatctt cgtgtcgctc aaggaggagg ccggggaggc
      421 ggttttttgc gtccgcgaca cgggaatcgg cattccgcgg ggcatgctcg accgcgtctt
      481 cgacatgttc ctccagatcg acaactcgct cgaccgggcc caagggggcc tgggcatcgg
      541 gctgacgctc gtccgcgaac tggtccacct gcaccgcggc atcgtcacgg tcaacagcga
      601 cgggccgggc caaggcagcg aattcaccgt ccgcattccc tgcctcgcgg ccggcctgca
      661 gccggaggac gccgtcacgg tcacggccga gatgccggcg ctcgttaaat gacggccgac
      721 tcgtcgatcg gccgcgactt gcgggctgcg ccgatgttct gctgcagcac cttcttgatc
      781 cactgcacgt tggcgtcgca ctcggcgatg gaccggcgaa acggatgaac gccgcgccgg
      841 atccacagga cggctgccag agccgtcacg gcgccgacca cgaggccgcc cgcgagggtg
      901 aatccgaagg cctgcgccgc cgtcaggcgc gtggcctcgt cgagcgcgag ggcaatcgtc
      961 gccaaggcga tcggaacgca gctcaagacc acggtgacgc cgaccaggag cagcacccct
     1021 ggataaacca attccttgga gagcagacgg gcatcgagcc aggccagccg cagctgcagg
     1081 tcgccaaggg tcagcgcgtc gtgcatcagc tccgacagat tgtgggccac ggccctgggc
     1141 gcggtgtagt cgcggtcggt cttatcgcgg ccgttggtct ggatagcggg gcgatcgacc
     1201 atggcggcgt gctcctctca atggacgtgg ggcctgggac gttccgggtc ggccggtgga
     1261 tgcgtctcgc cggcgtccgc cgcctggtgg ccgttcaaat agtgggccag ttgctgctta
     1321 cccaatgtca tggccgcttg ccagacgagc ggcccaacga ggcccaagac actgctgaat
     1381 aagctcttgc gcggcgccgg cggggtgagc ttcagcgcct gctcctgcag ggcgtcggca
     1441 atcgccgccg ggtccggccg gaaatcgcgg cgggccggga cgagcaagta cccggccgcg
     1501 gccgccgcgc ccaggcagac gacgggatag cggcgcacat agtgccgcca gtccgtcagt
     1561 acctgggcat tctccacgat ctcctcgacg tcctcgcgca gctcgcggcg gacctgccgc
     1621 atctgctgct ggatttcgtc ggcgctgtcg gacggcatcg gaggtctcct ggctccagac
     1681 catcgtcgtg tagcggctct gcgcgagccg ccgccttaca cacgcggcga gcggactcca
     1741 cccgtgatca ggctcacgac gaacaggatc aggaacagga cgaacagcac ctggccaatc
     1801 caggcgaagc tggccgcgat gccgccaaac ccgaatacgg cggcgatcaa cgccagtacc
     1861 agaaatgtta aagcccattg caacatggca gtttctcctt ggttcgcgtt tggtctttgc
     1921 gccgcggccc gcggcgcttc accttcccgc aataagcctg cgaatcgcgt gccggtgggt
     1981 agccgcgccc ggctgaaatg tccatactgg actgactcat agagagcgag gatggaatgg
     2041 ccggggttgt ggtcatcgac gacgatctga gcgtggtcgt cttgattcgc gccgcttgca
     2101 agcaggacaa cgtggcggtc cacgcggccg aaactccgga ggagggcttg gagctgctca
     2161 agcgggtgca gcccgatgtg ctgctgctcg acgtcgtatt gcccggcgtg tcggggctgg
     2221 aattgttcga gcggattcgg cggatcgacg ctcgcgtgcc ggtgatcttc atcacggcca
     2281 gcggcgagag cgaaacggcg atcgaggcca tgaagctggg agcgctcgac ttcctgctca
     2341 agccgctcga cgtcacccgc gtccgcgagc tgatcgggca ggcgctccgc atccgccagc
     2401 tgatggccac gccggtcgag ttgcccaggc ccgccgccgg cggcgacagc cagctcatcg
     2461 gccgcagccc gcagatgctc gaggtctata aggcgatcgg ccgcgtggcc ccgcagtcgg
     2521 tcaccgtcct gatctgcggc gaaagcggga ccggcaagga gctgatcgcg cgggccatct
     2581 atcagcacgg cgaccgcaag aacaagccct ttttggccgt caactgcgcc gccctctccg
     2641 agacgctgct ggagagcgag ctgttcggcc acgagaaggg ctcgttcacc ggggccgacc
     2701 accggcggat cggcaagttc gagcagtgca acggcggcac gatctttctc gacgaagtcg
     2761 gcgacatgcc gccgctgatg caggccaagc tgctccgcct gctccaggag cagcgcttcg
     2821 agcgggtcgg cggcaacgaa accatccaga ccgacgtgcg gatcatcact gccacgaacc
     2881 aggacctcga agccatgagc gccgccggca cgttccgggc ggacctctat taccggctca
     2941 acggcttcac catccgcctg ccgccgctgc gcgaccgggg cgacgacctg ctgctcctgg
     3001 tcgaggcctt cctctcgcga tattcgcaag agctggacaa gccgctgacg cgcgtctcgc
     3061 ccgacgcgat gaagatcctg ctggagtact cctggccggg caacatccgc gagttgcaga
     3121 acatcctgcg gaagtcgctg ctcaatttga gcggcccgat tttgctgccg gagtttctgc
     3181 cggaagaggt ccgccacccg tcgcaggcca gcggcggcga atccagcgcg gccagcggca
     3241 atggcggtct tccacatgat ttagcgccgt tcctggccga acgcatggcg gccggatccg
     3301 agaacatcta cgccgaggcg atcaactatc tggaccggtt cctggtgacg cgaatcctgc
     3361 agagcacgtc gggcaatcaa tccaaggcgg ccaaactgct gggcatcacc cgcggcagcc
     3421 tgcggaacaa ggtgcaggcg ctggacatca agatcgggca cgtcgtcaaa ggccctgagg
     3481 atgaagacgc tgccgacctg gccgcggagg ccggcggcta gatccgtccc agcagcacca
     3541 gtatcaggac gatcacaacg atcaacccca ggcccccgct agggccgtag ccccatgccc
     3601 ggctgtgggg ccacgcgggt atcgcaccca atagcagcag aatcaggatg atgagcaata
     3661 tcgtgccgag catggcttcg ctcctgtgga cgttcgcagg tggttagcaa taccactcac
     3721 ttgcacattg cgtgccgcga tcgcggaatc tggagcgaga tttagcgccg cgcaagggct
     3781 tcgatgattc cgcgcagctc gggcaagccg acgggttttt tcagatgccg gtcgaacccg
     3841 gcccccagcg ccgctcggcg gtcgctctcc tggccgtagc cggtgacagc caccaatagc
     3901 ggctgcctcg tcagcgggag tttgcggatc cttgcggcca gctcgtagcc cgagaggccc
     3961 ggcatgccga tgtcggagat caccgcgtcg gcggccagcg tgggcaatag cgctaaggcc
     4021 tcgtcgcccg attgggccac gtgcacttgc tgcccaagct tctccagcac gcgagcgagc
     4081 aggtgcgtag ccgacggttc gtcatcgacc acgagcaggc gaaaggccgg cagctcctca
     4141 gctgcagccg ctgggccgac tggttccgga tcggccacat cggccgctcg cccgtcggcg
     4201 gaattcctcg aagtctcgag aacgtcgtca acgagttgcc gcagttcgtc gagttgccgc
     4261 gacatgacgg ctgccagctc gcggacttgc tcgctgttgc ccggatcgag gacgatcaac
     4321 tggcaagacg cggtcagtgg cgcgagcgtg ttgcgcagct cgtgaacgac ggtctcgagg
     4381 ttctcgtgca ggccgcgatg tctggccatc gtcgtcacct gcgagccgcg ctttcgcgcc
     4441 gcggcgctga gccttcggcg tgcggcctca actcggccag cagttcctcg agcgccgacc
     4501 gcaattccgc cacggccgg
//
LOCUS       DASRJB010000640         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3570403, whole genome shotgun sequence.
ACCESSION   DASRJB010000640 DASRJB010000000
VERSION     DASRJB010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570403"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /gene="serA"
                     /locus_tag="VFB80_16970"
     CDS             <1..1294
                     /gene="serA"
                     /locus_tag="VFB80_16970"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZN35525.1"
                     /translation="ALSRRVAEAYHTLLAGKWDRKTFMGTQLADKTLGIVGLGRIGQE
                     VAKRAQAFQMRVLGYDPFLSAEGAAKLGITRVEKVRDMLPQIDYLTVHTPLTPETTNL
                     IDREAIALMKPGVRLVNCARGGIYNEEALVEGLKSGKLGGVALDVFVEEPCTKSPLFG
                     LPNVLCTPHLGASTEEAQTQVAVEAVELLINYFTKGEIRHAVNMAAIDPKTLEAIRGY
                     LDLAHRLGLLMSQWHSRGASACHLTYRGELAKKNTRLVTAAFCAGLLERALEEDVNIV
                     NSELLLRERGIRLTEESRSEPGAFSSSMSAEVASGPKDKFIASGTLFGNEMPRLIRLG
                     DFRLEAYLDGNLLVFTHNDVPGIIGTVGTIFGNHRVNIAQMSVGRTGPGGTAIGVLNL
                     DMVPPAAAVAEVLAHNDIQSVRVIELPVAGQLPTWLQA"
     gene            complement(1309..2196)
                     /gene="dapA"
                     /locus_tag="VFB80_16975"
     CDS             complement(1309..2196)
                     /gene="dapA"
                     /locus_tag="VFB80_16975"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZN35526.1"
                     /translation="MSRKGSAFAGLSVAIVTPFRGGQVDVKLLREQVEFQVAAGTQCL
                     CPVGTTGESPTLSHEEHERVISEVVQAARGRIKVMPGTGSNSTAEALRLTKWAAKEGA
                     DAALMVAPYYNKPTQEGFFQHYKAVAEQVGIPICVYNIPGRTGKNIEPETIARMAELP
                     NVALVKEATGSLDQASQILCTTDLTVLSGDDSLTLPLMAVGGEGVISVVGNIVPKDML
                     EMTTAYLQGDVAAAMKWHHKLFPLCRDTLGLATNPIPIKAAMKLLGRDTGELRLPMTP
                     LESALEGKLRKTLTNYGLM"
     gene            2299..2952
                     /locus_tag="VFB80_16980"
     CDS             2299..2952
                     /locus_tag="VFB80_16980"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN35527.1"
                     /translation="MRYFLPLVFAALPLLAPAQDKPAKFEPLFDGKSLNGWVVKGEPG
                     KDPPQGEWLAKDGVLSAKAGNSWLSTAKTYGDFVLRLEWRVPENGNSGVFLRVPVLKA
                     GEQPHVKGIEIQVLDDQGPQYQGKLQPWQHAGSIYGAVAAENSGYKGKGEWNSFEITC
                     RGGSIQVVMNGRKVASGEVEKIETLKTRPLRGYIGLQNHGTPVQFRNIEIQVLDSAP"
     gene            complement(2963..6559)
                     /gene="dnaE"
                     /locus_tag="VFB80_16985"
     CDS             complement(2963..6559)
                     /gene="dnaE"
                     /locus_tag="VFB80_16985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZN35528.1"
                     /translation="MAYAELHCKTNFSFLEGASHADELVARAAELGYAALAVTDRESL
                     AGVVRAHIAAKEAGLKLLIGAEVHPEDAPPAVLWATDRKSYGRLSRLLTVGRRRAEKG
                     QCRLTLADIAEHAAGLIAGVRVQGSGFRVQEVGVQEVGSRESGVGSRRSAGDDASRTP
                     YSVLSTPYEVPNSRPALRVFQPDPSPSPQPPALYADIFPGRAYLLAELFRGPDDAAEL
                     ARLVELSRKTGLPLVAAGDVHYHSPARQPLWEMLTATRLATTIDRLAEHRFANAQRHL
                     RPIDEIRTLFAAAPEALARTLEIAERCTFSLDELKYEYPEEFSQEGFTPLEYLEYLTW
                     QGARRRYPSGVPLKVRQLLDHELTLIKELRYEAYFLTVWDLVRFARSRGILCQGRGSA
                     ANSAVCYCLEVTSVDPERADVLFERFMSKERNEAPDIDVDFEHERREEVLQYIYQKYG
                     RDRAGMTAVVITYRTRSAVRDVGKALGLSLDRVDALAKQMEGRELDGYFYADKKTDKH
                     EHPQPPIVRSQGPGARGQPAQSDAAAPALPLSPSPPATPVDVLARRCLEAGLDPTSPL
                     GRRFVHLVTELVGFPRHLSQHVGGMVMTQGPLCELSPIENAAMEDRTVIQWDKDDLDE
                     LGILKVDCLALGMLTAIRKCFQLIERGEREASASRWLGSVPEVQAQRGAHAPRSPLTL
                     ATIPAEDPAVYDMICKADTIGVFQIESRAQMSMLPRLKPREYYDLVIEVAIVRPGPIQ
                     GNMVHPYLRRRSGQEAVTYPNDEIRAVLHKTLGVPLFQEQAMKLAVVAAGFTPGEADQ
                     LRRAMGAWRRPGIIDQFRKKLIEGMLQRGLAGEFAEQVFNQIRGFGEYGFPESHAASF
                     ALLVYVSAWLKCYYPAAFCAAIINSQPMGFYAPSQLIQGARAHGVRVLPVDVNDSGWD
                     CRLEPAVGDRESGVGERVQGSGFRVQGNAEPAAQYAALRPPTPDPRLPCLRLGLRLIT
                     GIPEAAAEKIEAARQAGPFMTLDDFTRRTRLSQPIVAKLAEADAFGSLRLDRRAALWE
                     ALAQEKRSKHQPLLAAVDDEEPAAELPPMPLVEQVFADYRTSGFSLKAHPISFFRGQL
                     DQLRVMPAGKLSEVRHGRRASVAGIVLVRQRPGTARGITFVTLEDETGLANLVIRPQI
                     WERYYKIARRSPAWIAHGVLESKDSVIHLVVHRLEDLSAKIGDLRAPSRDFH"
     gene            complement(6701..7552)
                     /gene="hisG"
                     /locus_tag="VFB80_16990"
     CDS             complement(6701..7552)
                     /gene="hisG"
                     /locus_tag="VFB80_16990"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZN35529.1"
                     /translation="MSNLRIGVPSKGRLSDLAGELLKQAGLSFRRQERSLFARVGEMP
                     IDVTFLRTDDIPVLCAEGAIDMGITGSDLVAESGASVVTRLALGVGKCRLALCVPDES
                     PITSPAQLAGKRVATSFPNVTRQYLRQHQADVHLVNLGGSVEIMITLGVADAIVDLVE
                     TGSTLAANRLRVLAELGKYESILVQNPARREAELADRVVRRLEGVVIARSWSLLEYNL
                     PRTKLAEAEKITPGFNSPTVNSLEDPAWCAVRVMVRRGEVIEVMEKLEAIGAHAILET
                     QINNCRL"
     gene            complement(7693..8031)
                     /locus_tag="VFB80_16995"
     CDS             complement(7693..8031)
                     /locus_tag="VFB80_16995"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZN35530.1"
                     /translation="MSSVLQKLMTVLEERKVNPPPNSYTARLYAGGDSKIASKITEET
                     QELIEAAGEPLEPGRRHMIHETSDLIYHLLVLLAHHGIKLEEIEAEISRRFGQSGLDE
                     KAARTVTVVK"
BASE COUNT         1422 a         2698 c         2764 g         1458 t
ORIGIN      
        1 cgccctctcg cgccgcgtgg ccgaggcgta ccacacgctg ctggccggca agtgggaccg
       61 caagacgttc atgggaacgc agctcgccga taagacgctg ggcatcgtcg gcttggggcg
      121 gatcggccag gaagtcgcca agcgggccca ggcgtttcag atgcgcgtgc tcggctacga
      181 tccgttcctg tcggcggaag gggcggccaa gctcggcatc acccgggtcg aaaaggtccg
      241 cgacatgctg ccgcagatcg actatctcac cgttcacacg ccgctcacgc cggaaacgac
      301 caacctgatc gaccgcgagg cgatcgccct gatgaagccg ggcgtgcggc tggtgaattg
      361 cgctcgcggc ggcatctata acgaagaggc actggttgag gggctcaaga gcggcaagct
      421 cggcggcgtt gcgctcgacg tgtttgtcga ggagccttgc accaagagtc cgctctttgg
      481 cctgccgaat gtgctttgca cgccgcacct cggggcgagc accgaagagg cccagacgca
      541 ggtcgccgta gaagcggtcg agctgctgat caactacttc accaaaggcg agatccgcca
      601 cgcggtgaac atggcggcga tcgaccccaa gacgctggag gcgatccgcg gctacctcga
      661 tctggcccat cgcctgggcc tcttgatgtc gcagtggcac tcgcgcggcg cgtcggcctg
      721 ccacctgacc taccgcggcg aactggccaa gaagaacacg cggctcgtca cggcggcgtt
      781 ctgcgcggga ctgctcgagc gggccctcga agaagacgtg aacattgtca attccgagct
      841 gctcttacgg gagcgcggca tccgtctgac cgaggaatcg cggagcgagc cgggggcttt
      901 cagctcatcg atgtcggccg aagtcgccag cggccccaag gacaagttca ttgccagcgg
      961 cacgctattt ggcaacgaaa tgccacggct gatccgcctg ggcgattttc gcctggaagc
     1021 ctacctcgac ggcaacctgc tggtgttcac ccacaacgac gtgccgggca tcatcggtac
     1081 ggtgggcacg atctttggca accaccgcgt taacatcgcg cagatgtccg tcggccgcac
     1141 gggccccggt ggcacggcga tcggcgtgct caacctcgac atggtgccgc cggcggccgc
     1201 cgtcgcggaa gtcctggccc acaatgacat ccagtcggtc cgcgtcatcg agctgccggt
     1261 agccggccag ctgccgacgt ggctgcaagc gtaagaagtc gagcggccct acatcaatcc
     1321 gtagttcgtc agcgtcttgc ggagcttgcc ttcgagggcg ctctccagcg gcgtcatcgg
     1381 caggcggagc tcgcccgtgt cgcggccgag cagtttcatc gcagccttga tcgggatcgg
     1441 attggtcgcc agcccgagcg tatcgcggca gagaggaaag agcttgtgat gccacttcat
     1501 cgcggcggcc acgtccccct ggagataggc cgtcgtcatc tccagcatgt ccttggggac
     1561 gatgttgccc acgaccgaga tcacgccttc gcctccgacg gccatcagcg gcaaggtcag
     1621 gctgtcgtcg ccgctgagca cggtgaggtc ggtggtgcag agaatctgcg aggcctggtc
     1681 gagcgagccg gtggcctctt tgaccagcgc gacgttcggc aactcggcca tgcgggcgat
     1741 cgtttcgggc tcgatgtttt tgcccgtgcg gccggggatg ttgtagacgc agatcggaat
     1801 gccgacctgc tcggcgactg ccttgtaatg ctggaagaag ccctcctgcg tcggcttgtt
     1861 gtagtagggc gcgaccatca gcgccgcgtc ggcgccttcc ttcgcggccc acttggtcag
     1921 ccgcagggct tcggcggtgc tgttgctgcc ggtgccgggc atgactttga tccggccgcg
     1981 ggcggcctgc acgacttccg agatcacccg ctcgtgctct tcgtgcgaaa gcgtcggcga
     2041 ttcgccggtc gtgccgaccg ggcagaggca ttgcgtgccc gcggcgactt gaaattccac
     2101 ctgctcgcgg agcagtttga catcgacctg cccgccccgg aacggcgtaa caatcgccac
     2161 cgacaatccg gcgaacgcgg aacctttgcg ggacatgaca aacctgcgcg gcggatagga
     2221 atgcggccag acaatttgca taataggcat ggcccgctca aatcgacaac ccgccgacaa
     2281 tttgccagga gtttcaccat gcgctatttc ctgccgctag tgtttgccgc tcttccgctg
     2341 ctggccccgg ctcaagacaa gccggccaaa tttgagccgc tgttcgacgg caaatccttg
     2401 aacggctggg tggtgaaagg cgagccgggc aaggacccgc ctcaaggcga atggctcgcg
     2461 aaggatggcg tgctcagcgc caaagcgggg aactcctggc tcagtacggc caaaacctac
     2521 ggcgacttcg tgctgcgttt ggagtggcgc gtgcccgaaa acggcaacag cggcgtcttt
     2581 ctgcgcgttc ctgttttgaa ggccggcgag cagccgcatg tgaaggggat cgagattcag
     2641 gtgctcgacg accaggggcc gcagtaccag ggaaagctcc agccgtggca gcatgcgggt
     2701 tcgatttatg gcgcggtcgc ggcggagaac tcgggctaca aaggcaaggg cgaatggaac
     2761 agctttgaga tcacatgccg cggcggatcg atccaggtcg tcatgaacgg ccgcaaagtg
     2821 gcctccggtg aggttgaaaa gatcgagacg ctcaagactc gtccgcttcg cggctacatc
     2881 gggttgcaga accacggtac gccggtgcag tttcggaata ttgagatcca ggtgctggat
     2941 agcgcgcctt agctcgccgg ctttagtgaa agtcgcgcga cggcgctcgc aggtcgccga
     3001 tcttcgccga caggtcctcg agtcggtgga cgacgaggtg gatcaccgag tccttgctct
     3061 cgagcactcc gtgggcgatc caggccgggc tgcggcgggc gatcttgtag taccgctccc
     3121 agatttgcgg gcggatgacg aggttggcca ggccggtttc gtcctcgagc gtgacgaagg
     3181 tgatgccgcg ggccgtgccg ggccgctgcc gcaccaggac gattcccgcg acgctggcgc
     3241 gccgcccatg gcggacctcg ctcaacttgc cggccggcat gacccgcagc tggtcgagtt
     3301 gcccgcggaa aaaactgatc gggtgggcct tcagcgagaa gcccgacgtg cggtagtcgg
     3361 caaagacctg ctcgaccagg ggcatcggcg gcaattccgc cgccggctct tcgtcatcga
     3421 ccgcggccaa gagcggctgg tgcttggacc gtttttcctg cgcgagcgcc tcccacagcg
     3481 ccgcacggcg gtcgagccgc agcgagccaa acgcgtccgc ctccgccagt ttcgcgacaa
     3541 tcggctggct cagccgcgtg cggcgcgtga agtcgtcgag cgtcatgaaa ggtcccgcct
     3601 ggcgagccgc ctcgattttc tcggcagctg cctccggaat gccggttatc aaccgaagcc
     3661 ccagccggag gcaagggagt cgggggtcgg gagtcggggg tcgcagggct gcgtactgag
     3721 cagccggttc cgcgtttccc tgaaccctga accccgaacc ctgaaccctc tccccgactc
     3781 ccgactcccg atccccgacc gccggctcca gccggcagtc ccacccgctg tcgttcacat
     3841 ccactggcag cacgcggacg ccgtgcgccc gggcgccctg gatgagctgc gagggagcat
     3901 aaaagcccat cggctggctg ttgatgatcg cggcgcagaa ggcggcgggg taatagcact
     3961 tcagccaggc ggagacatag acgagcaggg cgaagctggc cgcgtgcgac tcgggaaagc
     4021 cgtattcgcc aaagccgcga atctggttga agacctgctc ggcgaattcg ccggccaggc
     4081 cgcgttggag catgccctcg atcagcttct tgcggaactg gtcgatgatt cccggccgcc
     4141 gccaggcgcc catcgcccgg cggagctggt cggcctcgcc cggcgtgaag ccggcggcta
     4201 cgacggccag cttcatggcc tgctcctgga acagcggcac gccgagcgtc ttgtgcagca
     4261 cggcccggat ttcgtcgttg ggataggtga ccgcttcctg gccactgcgg cgccgcaggt
     4321 agggatgcac catgttccct tgaatcggac cgggccggac gatcgccacc tcgatcacca
     4381 ggtcgtaata ttcgcggggc ttcagtcgcg ggagcatgct catctgggcc cggctttcga
     4441 tctgaaagac gccgatcgtg tcggccttgc agatcatgtc gtagaccgcg gggtcttccg
     4501 ccgggatcgt ggcgagggtc agtggcgagc ggggtgcgtg cgcaccccgc tgggcttgaa
     4561 cttccggaac ggatccaagc catcgggagg ctgacgcctc ccgctcgcca cgctcgatca
     4621 gctgaaagca cttgcggatc gcagtgagca tacccagcgc gaggcaatcg actttgagga
     4681 tgcccagctc gtcgaggtcg tccttgtccc actggatgac cgtgcggtcc tccatcgcgg
     4741 cgttctcgat cggcgagagc tcgcacagcg gcccctgggt catcaccatt ccgcccacgt
     4801 gctgcgacag gtgccgcgga aagccgacca gctcggtcac caggtgcacg aaccgccgcc
     4861 ccagcgggct ggtgggatcg agaccggcct ccaggcagcg ccgggcgagg acatcgaccg
     4921 gcgtggccgg aggagagggg gagagaggga gagcgggagc ggcggcatcc gattgtgccg
     4981 gctggcccct ggctcctggc ccctggctcc tgacgatcgg cggctgcggg tgctcgtgct
     5041 tgtcggtctt tttgtcggcg tagaagtagc cgtcgagctc gcggccctcc atctgcttgg
     5101 cgagggcatc gacgcggtcg agcgagaggc cgagggcctt gcccacgtcg cggacggcgc
     5161 tccgcgtgcg ataggtgatg acgaccgccg tcattccggc ccggtcgcgt ccgtactttt
     5221 gatagatgta ctgcagcact tcctcgcgcc gctcgtgctc gaaatcgacg tcgatgtccg
     5281 gggcctcgtt ccgctccttg ctcatgaacc gctcgaagag cacgtcggcc cgctcgggat
     5341 cgacgctggt cacttccagg caatagcaga ccgccgagtt cgccgccgag ccacggccct
     5401 ggcacaagat gccgcgcgag cgggcgaacc gcacgaggtc ccagaccgtc aggaaatagg
     5461 cctcgtaccg cagctccttg atcaatgtca gctcgtggtc gagcagttgc cggaccttga
     5521 gcggcacgcc actcggatac cgcctccgcg ccccttgcca cgtcaggtat tcgaggtact
     5581 ccagcggcgt gaagccttcc tgggagaatt cctccgggta ttcgtatttc agttcgtcca
     5641 gcgagaatgt acaccgctcg gcgatctcca gcgtgcgcgc gagcgcctcg ggcgcggcgg
     5701 caaacagcgt gcggatttcg tcgatcggcc gcaggtgccg ctgggcgttg gcaaagcggt
     5761 gctccgccag ccggtcgatc gtcgttgcga ggcgcgtcgc cgtcagcatc tcccacaggg
     5821 gctgcctggc cggcgagtgg taatgcacat ctcccgccgc aaccagcggc aggccggttt
     5881 tccgcgacag ctccaccaat cgtgccagct ccgctgcatc atccggcccg cggaacaact
     5941 cggcgagcag atacgcgcgg ccggggaaga tgtcggcgta aagggctggg ggctgggggc
     6001 tagggctagg gtcgggctgg aacacgcgca gcgcgggccg gctattcggt acttcgtacg
     6061 gagtactaag tacggagtac ggagtacgcg acgcgtcatc gcctgccgac cgccgactcc
     6121 cgacccccga ctcccgactc ccgacctcct gaaccccgac ctcctgaacc ctgaaccccg
     6181 aaccctgaac cctcaccccc gcaatcaacc ccgccgcatg ttccgcaatg tccgccagcg
     6241 tcagccggca ctggcctttc tccgcccggc ggcggccgac agtgagcagc cgcgagagcc
     6301 ggccgtacga cttgcggtcg gtggcccaga ggacggcggg cggcgcatcc tcaggatgga
     6361 cttccgcgcc gatcagcagc ttgagcccgg cctccttcgc ggcgatatgg gcccgcacga
     6421 cgccggccag cgactcgcga tcggtcaccg ccagcgcggc ataccccagc tccgcagccc
     6481 gggccaccag ctcgtcggcg tgcgacgcgc cctccaggaa cgagaaattc gtcttgcaat
     6541 gcagctcggc gtaagccacg ggcggaagga ggcagtgtgg acgaactaac agtcgtactg
     6601 tccgcagacg ttcactatat ccacagtaag tgcaaatggc cagagtggca agctgtgagt
     6661 agtgagtggt ggggggtgac catcgaccac tcaccactca ctacaaccgg caattgttga
     6721 tctgtgtctc caggatcgcg tgcgcgccga tggcttcgag cttctccatg acctcgatca
     6781 cttcgccgcg gcggaccatg acgcggacgg cgcaccaggc ggggtcttcc aggctgttga
     6841 cggtcgggct gttgaagccg ggcgtgatct tctcggcttc ggccagcttg gtgcggggca
     6901 ggttgtactc gagcagcgac cagctgcggg cgatgacgac gccttccagc cggcggacga
     6961 cgcggtcggc cagctcggcc tcgcgtcggg cggggttttg aaccaggatc gattcgtact
     7021 tgcccagctc ggcaagcacg cggaggcggt tggccgcgag cgtgctgccg gtctcgacca
     7081 gatcgacgat cgcgtcggcg acgcccagcg tgatcatgat ctcgaccgag ccgcccaagt
     7141 tgaccaggtg cacgtcggcc tggtgctgcc gcaggtactg ccgcgtgacg ttgggaaagc
     7201 tcgtcgcgac gcgcttgccc gccagctgcg cggggctggt gatcggcgac tcgtccggca
     7261 cgcacagtgc gagtcggcac ttgccgacgc cgagggcgag ccgcgtgacg acgctcgcgc
     7321 cggattcggc gacgaggtcg ctgccggtga tgcccatgtc gatcgcaccc tcggcgcaga
     7381 gcacggggat gtcgtcggtc cgcaggaacg tgacgtcgat cggcatctcg ccgacgcggg
     7441 cgaacaggct ccgctcctgc cggcggaagc tgaggccggc ctgcttgagc aattcgcccg
     7501 ccagatcgct caggcggcct ttgctgggaa cgccgatacg gaggtttgac atggagtcag
     7561 gaggaagggg agttaaacac gaaggacacg cggggcacta agaaggaagg ggagagaggc
     7621 cgaagtgttc gctgaatcca ttcatcgttt cttctctctt cgtgttttcg tgcccttcgt
     7681 gtttaacctg cctcacttca ccacggtgac cgtccgggcg gccttttcgt ccaggcccga
     7741 ctggccgaac cggcgggaga tctcggcctc gatctcttcc agcttgatgc cgtggtgggc
     7801 cagcagcaca agcaggtgat agatcaagtc ggacgtttcg tggatcatgt gccgccggcc
     7861 gggctcgagc ggctcgccgg ccgcctcgat gagctcctgc gtctcttcgg tgatcttcga
     7921 ggcgatcttg ctgtcgccgc cggcatacag gcgggccgtg tacgaattgg gcggcggatt
     7981 gaccttgcgc tcctccaaaa ccgtcatcag cttttgcagg acgctggaca tggcagcgga
     8041 cttcgccagc gggctcttaa accagactcc agccccgttg caccgcccgc cgttctgcgg
     8101 ggactagagt agaatcatcg taagttcgcg tccaataacc cgcaatggct ggattcggca
     8161 cagagtaaac agaaggaaac gaaggacacg aagagaggcg aatgcgatga gccggcttct
     8221 cccttcgttt tcttcgttgc cttctgttcg tctcctcgct gcttccgttt gcaggacatg
     8281 cctgaagact tggaacccgc cccgcccgcg ctgaattgct ggtatctgac gggcccgacc
     8341 gc
//
LOCUS       DASRJB010000641         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3570983, whole genome shotgun sequence.
ACCESSION   DASRJB010000641 DASRJB010000000
VERSION     DASRJB010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570983"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..511
                     /locus_tag="VFB80_17000"
     CDS             95..511
                     /locus_tag="VFB80_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZN35531.1"
                     /translation="MFDLPWYHAGLKFTCSQCGDCCTGAPGHVWVNNEEIAALAKLRG
                     LAVAEFEEQFVRRVGTRKSLIEFPNGDCILFDGQTRKCTVYEARPRQCRTWPFWDSNL
                     TTAADWKQTCKVCPGSGQGQLHSLGEIEAQRQVIKV"
     gene            910..1284
                     /locus_tag="VFB80_17005"
     CDS             910..1284
                     /locus_tag="VFB80_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZN35532.1"
                     /translation="MTKKEIVKTISDEIGMTQLRTKEIVQKTFNAIVETLVDEHRIEL
                     RNFGVFEVKARAARKARNPRTGERVDVPEKFVVTFKPGKEMEEKVRELERRLISKGIS
                     LVEYAETRMAEHTARVKEDPAA"
     gene            1393..1611
                     /locus_tag="VFB80_17010"
     CDS             1393..1611
                     /locus_tag="VFB80_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35533.1"
                     /translation="MRRTILGLVLGLAACSQVGCALPIYSGDPAIRTKELINTSEDYK
                     MILDEWERIWFMDMPSHLSPRRVHGGIM"
     gene            1760..2707
                     /locus_tag="VFB80_17015"
     CDS             1760..2707
                     /locus_tag="VFB80_17015"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZN35534.1"
                     /translation="MPAASEFDVDLSVQLGRLKLRNPILTASGTFGYAREMERLIDFR
                     RLGGILPKTVTHEPRPGNTPWRTVETAAGLLNSIGLDNDGIEAFLEHHLPYLRELPTA
                     VIVSIAGRNHDEFVSMAARLDGLAGVAAIELNISCPNVAHGVDFGADWALCGRLVSAV
                     RERSRTPIIAKLTPNVTSIASIAKAAADAGADAISLINTVLGMAIDWRKRRPVLGNVV
                     GGLSGPAIKPIALRCVYQAAQAVKTPLIGIGGIATIDDVMEFLVAGATAVQIGTANYY
                     DPTVSMKLVDALPAALREAGVASVRELVGTLRTAPPTDH"
     gene            2728..3699
                     /gene="trpS"
                     /locus_tag="VFB80_17020"
     CDS             2728..3699
                     /gene="trpS"
                     /locus_tag="VFB80_17020"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZN35535.1"
                     /translation="MRVLSGIQPTGRFHWGNYFGAIRQYIDLQHGNEAYYFIANLHAL
                     TTVREPAVLRQNTLDAALDLLALGLDPAKATLFVQSDVPEVTELTWLLMSITPMGLLE
                     RCVSYKDKVSKGLVADAGLFTYPVLMSADILIYDSDVVPVGADQIQHIEVARDLAGSF
                     NHLFGETFVLPKYKVLESSAKVPGIDGEKMSKSYNNTLEIFEDVKPLRKKIMRITTDS
                     RPVEQPKPDFENDHLYQLYSLFATDAQRAEMAALYQRGNFGYGQVKTALADIAEQFFA
                     AARAKRAELAADLTHVEAILADGAARARKKASEVLRRAQNACGVKRT"
     gene            complement(3592..6510)
                     /locus_tag="VFB80_17025"
     CDS             complement(3592..6510)
                     /locus_tag="VFB80_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35536.1"
                     /translation="MRLWIIVSALVALLLVAEPAAARIDAEALVGQPFGVGTVTISGL
                     DASIDASRVLIQEKNGRVFYPTATPGVLGRILGQILGDPADRPASGVTINFLFRGEEP
                     LELTVYTPQSVPLTVRPAAPANSRVYERQVAQWWRNYNAYWRQLKAEDDHPPLVPTYL
                     TAMLSQRLGLEAPLVERLQSPKEKPTQATQSLELLLGMERLRLDTMRETMLGRGDFGQ
                     PLELPLPAPPAWQPLALPAELAETPIEPMALHVPHEWFYVRFGKFSNYLWMDYLIDEY
                     GGDISSMVTLRSYISALGDRAQDQLGLEKTILGDLLGDTVIADVAIVGSDTFTHEGGA
                     IGMLFQARNTKILDNDITQQQKRALDKAKASGATAQILEIAGKKVSLVSTPDNRLRSF
                     HVADGDFHLVTTSRAMVERFLRVNEGRGPLGKSAEFRYARQSMPLTRNDTIFAYFSSA
                     FFEGLLSPQYQVELERRMKSVTDMELLQMARLAAIGEGVRGETLEDLTAAAFLPRGFG
                     RRPDGSGPVITGTGIFDSRRGARGSFLPIPDVKIEGITRGEAQRVGALNAQLAQQWRR
                     MDPLMVGIQRYALDDKGRERVTIDANVNPLDESKYGWVLSMLGPPTRQMITPAEGDVI
                     SLQAAVRGGLLFDSIPAHHLFLGVQDTAPLSAIPPSGLLQTFNFLRSTPGYIGSWPRA
                     GFLDLLPFNLGGSTPDANGFSRLLFGLWRRQGAGFSVLSFDPQLLANITPQLRVAEAE
                     TEAQLRLHVADLSQSKIKPWITNLYYQRAITASAGNTRLLHQLNQQLHVPMDQAKEIA
                     EDLLDGQFVCPLGGEYQLVEDIRGGALTWQSTAWAKRNLASIPEDFEAPLLKWFRGLD
                     AHLIKIDGQITLHAELDMQRKPTAPKIELPLPFFNNLFGGGQKALKAKKDPKNDELPP
                     PLPPVKDPPKADPPKIELPGARDLGALDSAGVLGPPQDFAGLLAGAGSAVGEDSFDVG
                     "
BASE COUNT         1233 a         2093 c         2140 g         1166 t
ORIGIN      
        1 agggtcgaaa gttcggcgag gggatgatcg agttcgcgat tcggtagtaa gatgcgctgc
       61 atccgtctcc cgaccccgac tcccgactcc tggcatgttc gacctgcctt ggtatcacgc
      121 gggcctgaag ttcacttgct cgcagtgcgg cgactgttgc accggagccc cgggccacgt
      181 gtgggtgaac aacgaggaga tcgctgccct ggcgaaactc cgcggcttgg ccgtggccga
      241 gtttgaggag cagttcgtcc ggcgagtcgg cacgcgcaag agcctgatcg agttccccaa
      301 cggcgactgc atcctgttcg acggccagac ccgcaagtgc accgtatacg aggcccggcc
      361 gaggcaatgc cgcacgtggc cgttttggga ttcgaacctg acgacggcgg ccgactggaa
      421 gcagacctgc aaggtttgtc ccgggagcgg ccaggggcag ctgcactcgc tgggcgagat
      481 cgaggcccag cgtcaggtga tcaaggtcta gccgacttta gcggttagcg aatttcagcc
      541 gactttgcgg attggctgga gtttgcggat cgtaaggccg attgaatagg tccatgaccg
      601 cgcggctgca cacgctgcgc ggccggcgaa ttcgccgtta atccattccc tgcaacaagt
      661 tacggcgacg atgcggcccc ggcggccggc taattgacgc aactgctaat gaggaaacag
      721 tttacggaga gttgaggagg gcggctagca agccgctttt gacggtcgga atcgacgcaa
      781 gtccaatagt ttcgttgcgg cgagcttgac tctagtactt gtggcatcta cacttgggac
      841 gttcctgagg cttcggccaa aaaacgtttt cacggcgtca tttgccacca tgctggagga
      901 gagccagtcg tgaccaagaa agagatcgtc aaaacgattt cagatgaaat tggaatgacg
      961 cagttgagga ccaaggagat cgtgcagaag acgttcaacg ccatcgtcga gacgctggtc
     1021 gatgaacacc gcatcgagct ccgaaacttc ggcgtatttg aggtcaaagc ccgggcggcc
     1081 cgcaaggctc gtaatcctcg aaccggcgag cgcgtcgatg tgccggagaa attcgtcgta
     1141 accttcaagc caggcaagga gatggaagaa aaggtccgcg aattggagcg gcggttgatc
     1201 tcgaagggaa tcagcctggt cgaatacgcg gaaacccgaa tggcagagca cacggcacgc
     1261 gtcaaagaag atccggccgc ctgacccagc cagcaatcta cagtgcccag caacaaaaac
     1321 tcaaccagcg aaacaactta gggcaaagag caggccccgc gaacaccggc gactttcatg
     1381 gaggaaagat cgatgcgacg gacgattctg ggactggttt tgggcctggc ggcttgcagc
     1441 caggtcggct gtgcgttgcc gatctattcc ggcgatccgg cgattcgcac caaggaattg
     1501 atcaatacgt ccgaggacta caagatgatc ctggacgagt gggaacggat ctggttcatg
     1561 gacatgccga gccacttgag tccgcgccgc gtgcatggcg gcatcatgta gctaagtgcc
     1621 gcctgccaaa cggtttgaag cggtccgccg gcaggcaatt gtttgcactg gcggacgccg
     1681 gtacattcgg cgatggacgt gccgcagtcc gcgagacttc ctccactcgc ggcttcgcgg
     1741 tccgcagtca ttcgccctta tgcccgccgc cagcgagttt gacgttgatt tgtcggtcca
     1801 actcggccgg ctgaagctgc gcaacccgat cctgaccgcc tcgggaacat tcggttacgc
     1861 ccgcgagatg gagcggctga tcgattttcg ccggctgggc ggcatcctgc ccaagaccgt
     1921 gacgcacgag ccgcggccgg gcaacacgcc gtggcgaacg gtcgagacgg cggccgggct
     1981 gctcaactcg atcggcctgg acaacgacgg catcgaggcg ttcctcgagc accacctgcc
     2041 gtaccttcgt gagctgccga cggcggtcat cgtgagcatc gccggacgga accacgacga
     2101 gtttgtgtcg atggcggcgc ggctcgacgg actggccggc gtggcggcga tcgagctgaa
     2161 catttcgtgt cccaacgtgg cgcacggcgt cgatttcggc gcggattggg cgctttgcgg
     2221 ccggcttgtg tcggccgtgc gcgagcgttc gcggacaccg atcatcgcca agctgacgcc
     2281 caacgttacc agcatcgcat ccatcgccaa ggcggcggcc gacgcgggcg cggatgcgat
     2341 ctcgctgatc aatacggtcc tcggcatggc gatcgactgg cggaagcgcc ggccggtttt
     2401 gggaaacgtg gtcggcggac tgtccggccc ggcgatcaaa ccgatcgcgc tgcggtgcgt
     2461 ctatcaggcg gcgcaggccg taaagacgcc gctgatcggc atcggcggga tcgccacgat
     2521 tgacgacgtg atggagttcc tcgtcgccgg cgcgacggca gtccaaatcg gcacggcgaa
     2581 ctattacgat ccgacggtgt cgatgaagct ggtggacgcg ctgccggctg cgctgcgcga
     2641 agctggcgtt gcaagcgtgc gggaactcgt cggcacgttg agaaccgctc ctcccaccga
     2701 ccactgacca ccgacaactg accaccaatg cgcgttctct ccggcattca accgacgggc
     2761 cgctttcact ggggcaacta cttcggcgcg attcgccagt acatcgacct gcagcacggc
     2821 aacgaagcgt actacttcat cgccaacctg cacgcgctga cgacggtccg cgagccggcg
     2881 gtcctgcggc agaacacgct cgacgcggcg ctcgacctct tggccctcgg cctcgatccg
     2941 gcgaaggcca cgctgttcgt gcagtccgac gtgccggagg tgaccgagct gacgtggctc
     3001 ttaatgtcga tcacgccgat gggtctgctc gagcgctgcg tctcgtacaa ggacaaagtc
     3061 tccaagggcc tggtggccga cgcggggctg tttacgtatc cggtgctgat gtcggccgac
     3121 attttgatct atgactccga cgtggtgccg gtcggcgccg accagatcca gcacatcgag
     3181 gtcgcccgcg accttgccgg cagcttcaat catctcttcg gcgagacgtt cgtgctgccg
     3241 aagtacaagg tgctcgaatc ctcggccaag gtccccggca tcgacggcga gaagatgagc
     3301 aagagctaca acaacacgct cgagattttt gaggacgtca agccgctgcg gaagaagatc
     3361 atgcggatca ccaccgattc gcggcccgtc gaacaaccca agcccgactt cgaaaacgat
     3421 cacctctatc agctctactc gctcttcgca accgatgccc agcgggccga aatggccgcg
     3481 ctctaccagc gcggcaattt tggctacggc caggtgaaga ccgccctcgc cgacatcgcc
     3541 gagcagttct tcgccgccgc ccgtgctaaa cgcgccgagc tcgcggcgga cctaacccac
     3601 gtcgaagcta tcctcgccga cggcgctgcc cgcgcccgca agaaggccag cgaagtcctg
     3661 cggcgggccc aaaacgcctg cggagtcaag cgcacctaag tccctcgccc ccggcagctc
     3721 gatcttgggc gggtcggcct tgggcgggtc cttgacgggc ggcagcggcg gaggcagctc
     3781 gtcgttcttg ggatccttct tggccttcag cgccttttgg ccaccgccga agaggttgtt
     3841 gaagaagggg agcggcagct cgatcttggg cgcggtgggc ttgcgctgca tgtcgagctc
     3901 ggcgtgcagc gtgatctggc cgtcgatctt gatgagatgc gcgtccaggc ctcggaacca
     3961 tttgagtagc ggagcttcga agtcctccgg aatgctcgcc aggtttcgct tcgcccaggc
     4021 ggtcgattgc catgtgagcg cgccgccgcg aatgtcctcg acgagctgat actcgccgcc
     4081 cagcggacag acgaactgcc cgtcgagcaa atcctcggcg atttccttgg cctggtccat
     4141 tggcacgtgc agctgctggt tcaattgatg cagcaagcgc gtgttgccgg ccgaggcggt
     4201 gatcgcccgc tgatagtaga ggttggtgat ccagggcttg attttcgact gcgacaggtc
     4261 tgccacgtgc aggcgaagct gggcctccgt ctccgcctcc gcgacacgga gttgcggcgt
     4321 aatgttcgcc agcagttgcg gatcgaacga cagcaccgag aatcccgcgc cctgccgccg
     4381 ccagaggccg aacagcagcc gcgaaaagcc gttggcgtcg ggcgtgctgc cgccgaggtt
     4441 gaacggcagc aggtccagga agcccgcccg cggccaggag ccgatgtagc ccggtgtcga
     4501 gcggaggaag ttgaacgttt gcaacaggcc cgagggcgga atcgcggaca gcggcgcagt
     4561 gtcctgcacg ccgaggaaca ggtggtgcgc cggaatcgaa tcgaagagca gcccgccgcg
     4621 aaccgcggcc tgcagcgaga tcacgtcgcc ctcggccggc gtgatcatct gccgcgtcgg
     4681 cgggccgagc atcgacagca cccagccgta cttcgattcg tcgagtggat tgacgttggc
     4741 gtcgatggtg acgcgctcgc ggcccttgtc gtcgagagcg taccgctgga tgccgaccat
     4801 cagcggatcc attcgccgcc actgctgggc gagttgggcg ttgagcgcgc cgacccgctg
     4861 cgcttcgccg cgggtgatcc cttcgatctt cacgtcgggg atcggcagga agctgccccg
     4921 cgcgccgcgc cgcgagtcga agatacccgt acccgtaatc accgggccgc tgccgtcggg
     4981 ccgccggcca aatccgcgcg gcaagaaagc cgccgcggtg agatcctcca gcgtttcgcc
     5041 gcggacgccc tcgccgatcg ccgcgagccg agccatttgc agcagctcca tatcggtgac
     5101 ggatttcatc cgccgctcca actcgacctg gtactgcgga ctgaggaggc cctcgaaaaa
     5161 cgcggacgag aagtacgcga agatcgtgtc gttgcgagtt aagggcatcg actggcgggc
     5221 gtagcgaaac tcggccgact tgcccaacgg gccgcggcct tcgttcaccc gcaggaaccg
     5281 ctcgaccatc gcccgcgagg tcgtcacgag gtgaaagtca ccgtcggcca catggaacga
     5341 ccgcagccgg ttgtcgggcg tgctgaccag cgagactttc tttccggcta tctccaggat
     5401 ctgggccgtc gccccgctag ccttcgcctt gtcgagcgct cgtttctgtt gttgagtgat
     5461 gtcgttatcg agaatcttgg tgttccgggc ctgaaacagc atgccgatcg cgccgccctc
     5521 gtgcgtgaag gtgtcactgc cgacgatggc cacgtcggcg atgaccgtgt cgccgagcag
     5581 gtcgcccaga atggtcttct ccaggccgag ttggtcctgg gcccggtcgc cgagcgccga
     5641 aatgtagctg cgaagcgtca ccatgctgct gatgtcgccg ccgtattcgt cgatcaagta
     5701 atccatccac aagtaattcg agaacttgcc gaaccgcaca tagaaccact cgtgcggcac
     5761 gtgcagggcc atgggctcga tcggcgtctc cgccagctcc gccggcaacg ccagcggctg
     5821 ccacgccggc ggggccggca gcggcagctc cagcggctgc ccaaaatcgc cgcggccgag
     5881 catcgtctcg cgcatcgtgt ccagtcgcag ccgctccatg cccagcagca gctccagcga
     5941 ctgcgtggcc tgcgtcggct tttccttggg cgactggagc cgctcgacca gcggcgcttc
     6001 caggccgagt cgctgcgaga gcatcgccgt gaggtacgtc ggcacgagcg gcggatgatc
     6061 gtcctcggct ttgagctgcc gccaataggc gttgtagttc cgccaccact gggcgacctg
     6121 ccgctcgtac acgcgtgaat tggcgggtgc cgccggacgc accgtcagcg gcacggactg
     6181 cggggtgtaa accgtcagct ccagaggctc ttcgccgcgg aacaggaagt tgatggtcac
     6241 cccgctcgcc gggcggtcgg ctgggtcgcc gagaatctgc cccaagatcc gcccgagcac
     6301 gccgggagtt gccgtgggat agaagacgcg gccgttcttc tcctgaatga gcacccggct
     6361 ggcatcgatc gatgcatcca ggcccgaaat ggtcaccgtg cctacgccaa atggctgtcc
     6421 gaccagcgct tccgcgtcga tgcgggccgc ggcgggctca gcgacaagca acagggccac
     6481 cagggcactc acgatgatcc acagccgcat ggcaaatctc cttggccgag ccgccgtcaa
     6541 agtcggcgca gcttgccccg aattgtacag gtcgccgccc aagttgagac gcgagcggag
     6601 tgccggatct ttcgaaaaga cgtaaatcga tg
//
LOCUS       DASRJB010000642         4152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1876949, whole genome shotgun sequence.
ACCESSION   DASRJB010000642 DASRJB010000000
VERSION     DASRJB010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876949"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..368)
                     /locus_tag="VFB80_17030"
     CDS             complement(63..368)
                     /locus_tag="VFB80_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35537.1"
                     /translation="MADLKNPKLIYLKGFLFLVTGLTAAALLVAEAPTLKVALLLAIC
                     IWCFCRAYYFAFYVIEHYVDPQFKFAGLTSFVIYLVRRPLSQHRGAAASESSPHTAE"
     gene            463..813
                     /locus_tag="VFB80_17035"
     CDS             463..813
                     /locus_tag="VFB80_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HZN35538.1"
                     /translation="MLVHITTDNHIQGRERLVSEVEASVEESLARFAPQITRVEVHLS
                     DENGKKNGGDDKRCKLEARLAGLAAVGVTGSGSNLDQAVESALEKLHNLLDHKLGRLS
                     DKKGRPSYGGDGEA"
     gene            complement(824..1330)
                     /locus_tag="VFB80_17040"
     CDS             complement(824..1330)
                     /locus_tag="VFB80_17040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZN35539.1"
                     /translation="MTTIRPELLRDWPAVHAVHAAAFPTDAEARLVDCLRANGRAEIS
                     LVAELNGKIVGHILFSPVTIGGQAVAGALGLAPVAVIPSCQRQGIGGELIRAGLAACR
                     DVGCGLVVLLGHPEYYPRFGFQRASQFGLTNEYGADEAFMVLELRHGAIPAGGGLVQY
                     AAEFAEFA"
     gene            complement(1327..2106)
                     /locus_tag="VFB80_17045"
     CDS             complement(1327..2106)
                     /locus_tag="VFB80_17045"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN35540.1"
                     /translation="MDLHLQERVILVSGGAKGIGAGICRVLAAEGAIPVVIGRNAADN
                     HKLVTEIASAGGQAWSIEAELTRTEECERAVAAAVRQFGRIDGLVNNAGINDGVNLEH
                     GDTVRFLASLRKNLIHYYELAHFALSELKRSRGAIVNIGSKVAETGQGGTSAYAAANG
                     GRSALTREWAVELAKYGIRVNAVIVAECWTPMYETWLSSQSDPAAARRQIEARIPLGR
                     RMTTAAEIANTVAFLLSDCASHTTGQLIHVDGGYVHLDRAL"
     gene            2441..2740
                     /locus_tag="VFB80_17050"
     CDS             2441..2740
                     /locus_tag="VFB80_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35541.1"
                     /translation="MHAYRIVWEDEAKAREIELLVHYRLDAGAVNVREVRPTQVTLYS
                     APSERPIRSLPVHTATGRRLLTQAYLASRETEQTLEEEILAAHGLRDDSVAALAV"
     gene            complement(2737..3225)
                     /locus_tag="VFB80_17055"
     CDS             complement(2737..3225)
                     /locus_tag="VFB80_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35542.1"
                     /translation="MSEKELGQALLQLDSQKLAGVTDAREQTWKILERDRRRVWWLTA
                     LTITLWASAVAMVFAMLIAFGLLMPLQAKLRDDAQLARIGLTPADREVAQHKAAIIFQ
                     MITVGVTFSVGLLSLAALATVFLVTASRKATLRQINASLLEISQQVKELKQLSAEQKR
                     GG"
     gene            complement(3222..3791)
                     /locus_tag="VFB80_17060"
     CDS             complement(3222..3791)
                     /locus_tag="VFB80_17060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35543.1"
                     /translation="MSDQADRIYERLLVLRAQAGDAEALTELVERFSPRLRYFLRKLL
                     GEARHRGRSLQGDGAEDALQDVWLDVVRQLARLKDPQALVAWLYRIARDRAYGRLRKS
                     RRAEPLDETSVADSAADEGEFSPEDAARIHAALDQLPAEQREVLVLRFLEEMTYEQIA
                     SVVGCQLGTVRSRIHYGKQALRRILETNP"
BASE COUNT          743 a         1384 c         1291 g          734 t
ORIGIN      
        1 tgtcgcccgc acaattcatg cggcactctc ggaggccgca gtggatcttt caattcgcga
       61 tgctattcgg cagtatgcgg cgacgattct gaagctgccg ctccgcgatg ttgactgagc
      121 ggtctccgca ccaggtaaat cacgaacgaa gtcagcccgg cgaatttgaa ctgcggatcg
      181 acgtagtgct cgatcacgta aaacgcgaag tagtacgcgc ggcagaagca ccagatgcag
      241 atggctaaca gcagggcgac cttgagcgtc ggcgcttcgg ctacgagcag cgccgccgcg
      301 gtcaggcccg tgaccaggaa caggaagccc ttgagataga tcagcttggg gttcttcaaa
      361 tcggccatgg cagcggtcta cttggacgcg gattattcct gccattctac gctctctacc
      421 ctgcaccccg ccggcagcta gaatcgggat ggaggaaccg ccatgttggt gcacatcacg
      481 acggacaacc acatccaggg gcgcgagcgg ctggttagcg aggtggaagc ctcggtcgag
      541 gagtcgctgg cccgtttcgc tccgcagatc acgcgggtcg aggttcacct cagcgacgaa
      601 aacggcaaga agaacggcgg cgacgacaag cgctgcaagc tggaagcccg cctggccggc
      661 ctggccgccg tcggcgtgac cggctcgggc tcgaacctcg atcaggccgt cgagtccgcg
      721 ctggagaagc tccacaacct gctggaccat aagctcggac gcctctcgga caaaaagggc
      781 cgaccgagct atggcggcga cggcgaagcc tgattcgcca gcgctaggca aactccgcga
      841 actcagcggc atactggacg agcccgcccc ctgcgggaat cgcaccatgc cgcagctcca
      901 ggaccatgaa cgcctcatcc gcgccgtatt cgttcgtcag gccgaactgg ctggctcgct
      961 gaaagccaaa ccgcggatag tattccggat gccccagcag cacgaccagg ccgcagccca
     1021 catcgcggca ggccgcgagc ccggcgcgaa tcagctcgcc gccgattccc tgccgctggc
     1081 agctggggat cacggctacc ggcgcgagcc ccagcgctcc ggccaccgct tgcccgccga
     1141 tcgttaccgg gctgaagaga atgtggccga cgattttccc attcagttcc gcgaccagcg
     1201 agatctccgc ccggccattg gccctcaagc aatcgacgag ccgggcctcg gcgtcggtcg
     1261 gaaacgcagc ggcgtggacc gcgtggacgg cgggccagtc gcgaagcagc tcgggtcgaa
     1321 tcgtcgtcat agcgcccggt cgaggtggac gtagccgccg tctacgtgaa tcagctggcc
     1381 cgtggtgtgg ctcgcacagt cagacaggag aaacgccacg gtattggcga tctccgccgc
     1441 cgtcgtcatc cggcggccga gtggaattcg cgcctcgatc tgccggcggg ccgccgcggg
     1501 atctgactgc gacgacagcc acgtctcgta catcggcgtc cagcactccg cgacgatcac
     1561 cgcgttcacc cggattccat acttcgccag ctcgaccgcc cactcccgcg ttagcgcgct
     1621 acggccgccg ttggcagcgg cataggcgct cgtcccgccc tgccccgtct cggccacctt
     1681 cgagccgatg ttcacgatcg ccccgcggct ccgtttcagc tcgctcaggg cgaagtgagc
     1741 cagctcgtag tagtgaatca ggttcttccg cagcgacgcc aggaaccgga ccgtgtcgcc
     1801 gtgttccagg ttcacgccgt cgttgattcc cgcgttgttc accagcccgt cgatgcggcc
     1861 gaattgccgc acggccgcgg ccacggcccg ctcgcactct tcggtgcgcg tcaactcagc
     1921 ctcgatcgac caggcctggc cgcctgcgct ggcaatctcc gtcacgagct tgtgattgtc
     1981 ggcggcgttg cggccgatga cgactggaat cgctccttcg gccgccaaca cgcggcaaat
     2041 gcccgctccg attcctttcg ccccaccgct gaccaggatg acgcgctctt gcagatgcag
     2101 gtccatgccc gaaagcgtat caggttatgc ggccgccgag cgggcgattg tctccccgta
     2161 gcccgcaggc cgtttggtcc gcaccatccg caaaggcggc atcacgcgtg agttgttaag
     2221 gtgaatccag acgccgttat tagttgattg cgtggctcgg agtcgcattg gaccaggtcg
     2281 gcggtgcgcg ccgcggaatc ttcatgaatt gcagcatttc ttgggcaatt aattgagtag
     2341 cacttcgcgg cgaccgacgc tttacttaca ctgagtagct tccacttttg gaaaaaagta
     2401 cgccttaccg gccgcgggtc cgcggccacg gagagaagag atgcacgctt atcgcattgt
     2461 ttgggaggac gaggccaaag cccgcgaaat tgagctgttg gttcactatc ggctggatgc
     2521 cggtgcggtg aacgtccgcg aggtccgccc gacgcaggtc accctctaca gcgcaccatc
     2581 agagcggccc atccgctcgc tgcccgtgca caccgcgacc ggccgccggc tgctgaccca
     2641 ggcttacctg gccagccgcg agaccgagca gacgctcgag gaggaaatcc tcgccgccca
     2701 tgggctccgc gacgactcgg tcgcggcctt ggccgtctaa ccgccgcgct tctgttccgc
     2761 gctcaactgc ttcagctcct ttacctgctg agaaatctcc agcaggctgg cgttgatctg
     2821 ccgcagggtg gccttgcggc tggcggtgac caggaacacc gtggccagcg cggccaggct
     2881 cagcaggccg accgagaagg tcacgccgac ggtgatcatc tggaagatga tcgcggcttt
     2941 gtgctgggcc acctcgcggt cggcgggcgt caggccgatg cgggccagtt gcgcatcgtc
     3001 gcggagtttg gcctgtagcg gcatcaagag gccaaaggca atgagcatcg cgaagaccat
     3061 cgcgacggcg ctggcccaca gcgtgatggt cagcgccgtc agccaccaca cgcggcggcg
     3121 gtcgcgctcc aggattttcc aggtctgctc gcgggcgtcg gttacgccgg ccagcttctg
     3181 cgagtcgagt tgcaagaggg cctggccgag ttctttttcg ctcatgggtt cgtctccaaa
     3241 atgcgccgca gcgcctgttt gccgtagtgg atccgcgagc ggacggtgcc gagctggcag
     3301 ccgacgacgc tcgcaatctg ctcgtaggtc atctcttcca aaaaccgcag cacgaggacc
     3361 tcgcgctgtt ccgccggcaa ctggtcgagc gcggcgtgaa tccgcgcggc gtcctcgggc
     3421 gaaaactcgc cttcgtccgc cgcggaatcg gccacgctgg tctcgtccag cggctcggcc
     3481 cgtcgcgact tccgcagccg gccgtaggcc cggtcccgcg cgatgcgata cagccaggcc
     3541 accagtgcct gcgggtcctt cagccgcgcg agttgccgga cgacgtcgag ccagacgtcc
     3601 tgcaaggcgt cttcggctcc gtcgccctgt agggaacgcc ctctgtggcg tgcctcgccc
     3661 agcaacttcc gcaggaagta ccgcagtcgc ggcgagaacc gctcgaccag ctcggtcagc
     3721 gcctccgcat cgcccgcctg agcccgcagc accagcagtc gctcgtaaat gcggtcggcc
     3781 tgatcgctca tgcggctaac gctaacggtg ccggtcaaaa ctcgccaaac atcaggttgc
     3841 ggcgggcgac gaaaagttca tcttcgcccc tgttcagcag cgagaccctg aacagtcctc
     3901 tttcgggtgg attcgcgatt gggccgatcg cagcctctga gggcattgcc gagtcgtaac
     3961 ccattgcctg aaaatccttt agcgtcaatt gctatgcgat cgaatcacat tagagtttga
     4021 agccgtatta actgttgctc gggcttctga acaggtctcg cacgcggcga aacctgcgcg
     4081 atcttgggaa agccatctac cactcttcaa ggagcgatag tgcctgcagc gcgccaaaat
     4141 ggcagtcgcg gg
//
LOCUS       DASRJB010000643         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1876989, whole genome shotgun sequence.
ACCESSION   DASRJB010000643 DASRJB010000000
VERSION     DASRJB010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876989"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="VFB80_17065"
     CDS             <1..1691
                     /locus_tag="VFB80_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin D"
                     /protein_id="HZN35544.1"
                     /translation="LPNFHAFFGPENWFYLAITMAVVKVLHEFGHGLSCKHFGGECHE
                     LGAMMLVFTPALYCNVSDSWMLPNKWHRAAIGAAGMYVELFLASCATFLWWYSSPGLL
                     NSICLSVMFICSVSTVMFNGNPLLRFDGYYILMDLLEIPNLRQKATEITRRFFVWLCL
                     GIEQPENPFLPHKHQWAFGIYTIAAVIYRWLVVFSILFFLNKVFEPYGLKIIGQLIAL
                     SGFVGLVVQPVWELGKFFYTPGRMNKMKHERLYATVGVLAAAVAGVLFVPLPFSVKCA
                     FEVQPRGGQPVYTTVAGQIMTPVEYKPGQKVAQGTVLMQLQNAELEMELLELKGRCDE
                     AEQAVRNLKQKQRYGDPAAVDQLDTAEKIAASTRHLYEGKRTEFERLTIVAPVGGTII
                     PPPARHDKAAAAQGRLPSWSGTPFDPHNTGAMITPTELICQIGDPADMEAVLIVDQAY
                     VGLVQKKHQVKLLLESSSRRSLYSEVEDIAPTEMKVASRGMSSQGGGRLETKTDSAGM
                     VRPLSTSYQVRVPLDDAPEELAVGMQGQARIYTGWQPLGRRLFRYCAKTFHFDL"
     gene            1853..2200
                     /locus_tag="VFB80_17070"
     CDS             1853..2200
                     /locus_tag="VFB80_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZN35545.1"
                     /translation="MSEKLEKEIVETPPAAPAQQQQMVQVEVDDSHVTASYANFCRVT
                     GSPEELIIDFGLNPQPVGIPKNPIGVSQRVIVNYYTAKRLLHALSLSVQRHESVFGVL
                     ETDIQKRVKPMTR"
     gene            complement(2301..>3339)
                     /locus_tag="VFB80_17075"
     CDS             complement(2301..>3339)
                     /locus_tag="VFB80_17075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN35546.1"
                     /translation="KEIGPEEFRAPRDKEKADVKRLANVSKPTLTIFQPPQEKRNGAA
                     VIVCPGGGYNILAWDLEGTEVAEWANSLGLTAFVLKYRVPKREGDTANTLPLMDAQRA
                     MSLVRSKASDYGIDPARIGMLGFSAGGNLTAATCLKFSERQYDKLDAVDDVSCRPDFG
                     VLIYPAYLVDNEGKLKPEYQPTKETPPMFFAHALNDGVTPDSSIALTRALKAVGVHAE
                     LHVYDGGGHGFGLRKSEFPVHTWPARCGEWLAVRGFLKPAAPTASLKGTVTIAGAPLP
                     EGKVELHAAGAEPVVLEIRNGAYSATVPIGKFSVTILGKGVPEKYSAPAVSPLKVEIT
                     ATANEIRFDLK"
BASE COUNT          577 a         1086 c         1052 g          624 t
ORIGIN      
        1 gcctgccgaa ctttcacgcg ttcttcggcc cggaaaactg gttctacctg gcgatcacga
       61 tggccgtggt caaggtcctg cacgagttcg gccacggcct gtcgtgcaaa cactttggcg
      121 gcgagtgcca cgagctggga gcgatgatgc tcgtcttcac gcccgccctg tattgcaacg
      181 tctccgactc ctggatgctc cccaacaagt ggcaccgggc ggcgatcggc gcggcgggca
      241 tgtatgtcga gctgttcctg gcctcctgtg cgacgtttct ctggtggtac agctcgccgg
      301 gtctgctcaa ctcgatctgc ctcagcgtga tgttcatctg ctcggtcagc acggtgatgt
      361 tcaacggcaa cccgctgctg cggttcgacg gctactacat cctgatggac ctgttggaaa
      421 tcccgaacct gcggcaaaaa gcgaccgaga tcacgcggcg gttcttcgtc tggctctgcc
      481 tgggcatcga gcagccggaa aatccgttcc tgccgcacaa gcaccagtgg gcgttcggca
      541 tctacacgat cgcggccgtg atctaccgct ggctggtcgt gttctcgatt ctgtttttcc
      601 tgaacaaggt gttcgagcct tatgggctca agatcatcgg ccagttgatc gccctctcgg
      661 gcttcgtcgg gctggtcgtg cagccggtct gggagctggg caagttcttc tataccccgg
      721 ggaggatgaa caagatgaaa cacgagcggc tgtacgccac agtgggcgtg ctcgcggcgg
      781 ccgtggccgg cgtgctgttt gtgccgctgc ccttctcggt gaagtgcgcc ttcgaagtgc
      841 agccgcgggg cgggcagccg gtctatacga cggtcgccgg ccagatcatg acgccggtcg
      901 agtacaagcc cggccagaag gtcgcccaag gcaccgtgct gatgcagctc cagaacgcgg
      961 agctggaaat ggagctcttg gagctgaagg gtcgctgcga tgaagccgag caggccgtcc
     1021 gcaacctgaa gcagaagcag cgctacggcg atccggcggc cgtcgatcag ctcgatacgg
     1081 ccgagaagat cgccgcctcc acccgccacc tgtacgaggg gaagcggacg gaatttgagc
     1141 ggctcacgat cgtggccccc gtgggcggca cgatcattcc gccgccggcc cgccacgaca
     1201 aggccgccgc ggcccagggc cggctgccga gctggtcggg cacgcccttc gatccgcaca
     1261 acaccggcgc gatgatcacg ccgaccgagt tgatctgcca gatcggcgac ccagctgaca
     1321 tggaagccgt gctgatcgtc gatcaggctt acgtcggcct ggtccaaaag aagcaccagg
     1381 tcaagctgtt gctcgaatcg tcgtcgcggc ggtccctgta cagcgaggtc gaagacatcg
     1441 cccccacgga gatgaaggtc gcctcccgcg gcatgtcgtc gcagggcggc ggccggctgg
     1501 agacgaaaac cgattcggcc ggcatggtcc ggccgctgag cacttcgtat caggtccgcg
     1561 tgccgctgga cgatgccccg gaagagctgg ccgttggcat gcagggccag gcccgcatct
     1621 acaccggctg gcagcccctg ggccgccggc tgtttcggta ttgtgccaag acgtttcact
     1681 ttgacctgtg agacataacc ggcgagcggg aggcgtgagc ttcccgatag cgcagcggac
     1741 ggagacgccg caccatcggg aagctcacgc atcccgctcg ctaccctgag gaaaccccgg
     1801 ccgcgtcggc cgacaaactt cttgttttcg agtaaccttt tggagcagaa cgatgtccga
     1861 gaaactggag aaagagatcg ttgagacccc gccggccgcc ccggcccagc agcagcagat
     1921 ggtgcaagtc gaggtcgatg actcccacgt caccgcctcc tacgccaact tctgccgcgt
     1981 gaccggctcg ccggaagagc tgatcatcga cttcggcctc aacccgcagc ccgtcggcat
     2041 tccgaagaac ccgatcggcg tcagccagcg cgtgatcgtg aactactaca ccgccaagcg
     2101 gctgctgcac gccctgtcgc tgtccgtgca gcggcacgag tcggtgttcg gcgtcctgga
     2161 gaccgacatc cagaagcgcg tcaagccgat gacgcggtag gcgtacctcg cgctgtaggg
     2221 aacgccctct gtagcgttcc cgaactgcga acaaaatcaa acccgcagcc tcgccctatg
     2281 ggcgtcgccg cgggtttttt tcatttgaga tcgaagcgaa tttcgttcgc tgttgcggtg
     2341 atttcaacct ttagcggcga aacagccggt gcgctgtact tttcgggaac acccttgcct
     2401 aagatcgtta cgctgaactt tccaatgggc acggtcgcag agtacgcgcc atttcgaatc
     2461 tccagcacca cgggctcagc gccggcagcg tgcaactcga ctttgccctc cggcagaggc
     2521 gcgccggcga tggtgaccgt tcccttcagt gacgctgtcg gcgccgccgg cttcaagaag
     2581 ccgcggacag ccagccactc gccgcaccgc gcgggccagg tgtgcacggg aaactcgctc
     2641 ttccgcaggc cgaagccgtg gccgccgccg tcatagacgt gcagctcggc gtggacgccg
     2701 acggccttga gcgctcgcgt cagggcgatg ctgctgtcgg gcgtcacgcc atcgttcagg
     2761 gcgtgggcga agaacatcgg cggcgtttcc ttcgtcggct ggtactcggg cttgagcttc
     2821 ccctcgttat cgacgaggta ggccggatag atcagcacgc cgaaatcggg ccggcaggag
     2881 acgtcatcca cggcgtcgag cttgtcgtat tgccgctcgc tgaatttgag gcaggtcgcc
     2941 gcggtcaagt tgccgcccgc cgagaatccc agcatgccga tccgcgcggg gtcaattcca
     3001 tagtcgctgg ccttgctccg caccaggctc atcgcccgct gggcgtccat gagcggcagc
     3061 gtattcgccg tgtcgccctc gcgcttgggc acgcggtatt tcagcacgaa agccgtcagg
     3121 cccagcgagt tggcccactc ggcgacttcg gtcccttcca ggtcccaggc caggatgttg
     3181 tagccgccgc caggacagac aatcaccgcg gccccgtttc gcttctcctg cggaggctga
     3241 aatatcgtga gcgtcggctt gctgacgttc gcaagtcgct tcacgtccgc cttttccttg
     3301 tcccgcgggg cgcggaactc ctcggggccg atttcctta
//
LOCUS       DASRJB010000644         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2154693, whole genome shotgun sequence.
ACCESSION   DASRJB010000644 DASRJB010000000
VERSION     DASRJB010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154693"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFB80_17080"
     CDS             <1..716
                     /locus_tag="VFB80_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35547.1"
                     /translation="YLGWSGDGHINRFNITHAFYWVLGKDELNPIAGEPQDINAQMFA
                     VELSYDRDWMRFRTSYFFASGDSDVSFGNRVATGFDTILDNPNFAGGQFSYWNRQQIK
                     LFGVNLVNRLSLVPDLRSSKFQGQSNFVNPGLQLINLGVDADLTPRLKLIGNCNFLWF
                     NQTEVLQTFVFQDDIATNIGVDLSLGAEYRPLLSNNIIMVGGVSCLIPGGGFRDLYNP
                     LVGRVDALAAGFLNVIMTY"
     gene            732..>4776
                     /locus_tag="VFB80_17085"
     CDS             732..>4776
                     /locus_tag="VFB80_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35548.1"
                     /translation="MQRVIAIFIALSLAADLPAGEPPGPWKLLPIRLWSQPARPAQNA
                     KPKTAVAPASYQETLPPPNAPLPLMEPFPLPPGTPAAEELYRQTADDALRKSWGCVNC
                     HQGVQDMHDLPTVKLGCIDCHGGNPDASTKEAAHVHPCQPYGWPSSANPVRSYTLLNH
                     ESPEFIRFVNPGDLRVAHISCGACHPKEVLEVKKSMMTHGCMLWGSALYNNGAVPNKA
                     ARYGESYSMCGVPQRVQTIWKAGDIELMQYEVARKAVVPFLDPLPRFEITQPGNVLRI
                     FEQGGRFRPEVGIPERLEEPGRPRTRLSNRGLGTENRTDPVFIGLQKTRLLDPTLNFL
                     GTNDKPGDYRSSGCSACHVVYANDRSPVHSGPYAKYGHMGLAAAEPDGQFVKNVDPTI
                     PKHESGHPIAHRFSVGMPTSQCIVCHMHPGTNVVNAYLGTMWWDNETDGELMYPPQEK
                     LPTSEQLAESLAFNPDETAARGNWSDPQFLANLTDLNSVTRHTQFADFHGHGWAFRAV
                     FKKDRAGHLLDWQGRRVAAVTNDKLREAVRPPTEDEKTVGKQRVGTPVHLMDIHLEKG
                     MHCVDCHFQQDNHGNTKLYGEVRAAIEIQCIDCHGTAGETAPERIERTGRLATSGPAA
                     PPGGTNLLTLRTPHTPRQARFELQPDAPTSDGKPRYKLIQRSMVEPDRKWEVKQTAYT
                     IRPDHPDYNEKSHAAKTVRWDAAGELLWGGQPDPQEEIGGRKCAHQSNTISCIACHSS
                     WNPSCFGCHLPQKANQKTPSLHNEGDVSRNLVRYNFQTLRDDVFMLARDGLATGNRIG
                     PARSSCAVHVSSYNANRENVYHQQQTISGCGLSGIAFSTNVPHTVRGGSRSLLNPAPG
                     TSETKSCSDCHPSKNNDNNALLAQLLMQGTNYTNFMGKYCWVAAGEHGLEAVVVTEQD
                     EPQAVIGSTLHRVAYPERHRHHLERGGELEHAHEHPGVDILDQLRHPLQKPEILQVQA
                     RGEFLYAACGSGGFRVFDMAFIDHKGFSERITTAPVSPLGQQLYVPTKYATAVAAPTT
                     LAPDPTRQQLPENREQSVHALYAYIYVADKYEGLILVNAATLLDGNPLNNFLSRSLTY
                     NPGGLLAGARHITIAGTFAYVSCDAGLVVIALDDPVNPYVTAVIDAETLPQAGVSQVQ
                     FRYAFVCAADGLHVLDVTDLAQPKPAGHLPLADCRSVYVARTYAYIAAGHQGLVIADI
                     TRPDRPAIDQIFDAGGCIDDLHDVKLGITYTSQFAYLADGQNGLRVVQLTSPDTPGNS
                     GFSPRPTPQLVATRKLPRGGHALFVSEALDRDRAVDESGNQIAVFGRIGARPLLREEQ
                     QRMYLRPATDGKGPRRPWWVSDNVFDRE"
BASE COUNT          928 a         1613 c         1454 g          781 t
ORIGIN      
        1 actacctggg ctggtcgggc gatggacata tcaaccggtt caacatcacg cacgccttct
       61 attgggtgct cggtaaggac gaactgaatc cgatcgccgg cgagccgcaa gatatcaatg
      121 cgcagatgtt tgcggtggag ctctcgtacg accgcgactg gatgcggttt cggacttcct
      181 atttcttcgc tagcggcgac tccgacgtga gctttggcaa tcgcgtggcc acgggctttg
      241 acaccatcct cgacaatccg aactttgccg gcggccaatt cagttattgg aatcggcagc
      301 aaatcaagct gtttggcgtc aacctggtca atcgcctgag cctcgtgccc gatctgcgtt
      361 ccagcaagtt ccagggccaa tccaatttcg tgaatccggg cctgcaattg atcaacctgg
      421 gcgtcgatgc cgatctgacg ccgcgcctca agctgatcgg caactgcaac ttcctgtggt
      481 tcaaccagac cgaggtgctg cagacgtttg tctttcaaga tgacattgcg accaatatcg
      541 gcgtcgatct gagcctgggc gccgaataca ggccactcct gagtaacaac atcattatgg
      601 tcggcggcgt ctcctgcctg atccccggcg gcggattccg cgacctttat aacccgctcg
      661 tcggacgcgt ggatgcactg gcggccggat tcctgaatgt gatcatgacc tattagaact
      721 ggaatgcacg gatgcaacgc gtaatagcga tcttcattgc gttgagcctg gccgcggact
      781 tgccagcggg agagccgccg ggaccgtgga aactgctgcc gatccgcctc tggtcgcagc
      841 ctgcgcggcc cgcacagaat gcgaagccca aaacggcagt ggcgccggcg tcctatcaag
      901 agacgctgcc gccgccgaat gcgccattgc ctttgatgga gccgtttcca ctgccgccag
      961 gaacacccgc ggcagaagaa ctgtatcgcc agaccgcgga cgacgccctg cgaaagagtt
     1021 ggggctgtgt gaactgccac cagggcgtcc aggacatgca tgacctgccc accgtgaagc
     1081 tcggctgcat cgattgccac gggggcaacc ccgacgcctc gaccaaggaa gccgcacacg
     1141 tgcatccctg ccagccgtac ggctggccct cgtctgcgaa tcccgttcgg tcttatacgt
     1201 tgctcaatca cgagagcccg gaattcattc gcttcgtcaa tccgggcgat ctgcgcgtcg
     1261 cgcatatcag ttgcggcgca tgccatccga aggaggtttt ggaagtcaag aagagcatga
     1321 tgacccacgg ttgcatgctg tgggggtccg ccctctataa caacggcgcc gtgccgaaca
     1381 aggctgctcg ctacggcgaa agctacagca tgtgcggcgt gccgcagcgc gtgcagacga
     1441 tctggaaggc aggcgacatc gagctcatgc aatatgaagt cgcccgcaag gccgtcgtgc
     1501 cgttcctcga tccgctgccg cgattcgaga ttacgcagcc gggcaacgtg ctccgcatct
     1561 ttgagcaagg cggtcggttc cggccggaag tcggcattcc cgagcgactg gaagagccgg
     1621 gccgcccgcg cacgcggctc tccaaccgcg gcctgggcac cgagaatcgc acggacccgg
     1681 tcttcatcgg gcttcaaaag acgcggctgc tcgatccgac gctgaatttc ctgggcacga
     1741 acgacaagcc gggcgactat cgctccagcg gctgctcggc ctgccacgtg gtctatgcca
     1801 acgaccgctc gccggtgcac agcggcccgt atgccaaata cggacacatg ggtctggccg
     1861 cggccgagcc cgacgggcag ttcgtcaaga acgtcgatcc gaccattccc aagcacgaat
     1921 cgggccatcc gatcgcccac cggttcagcg tcggcatgcc gacgagccag tgcattgtgt
     1981 gccacatgca tccgggcacc aacgtcgtca acgcctatct gggcacgatg tggtgggaca
     2041 acgagaccga cggcgagctc atgtatccgc cccaggaaaa gctgccgacg tcggagcagc
     2101 tggctgagtc gctggccttc aatcccgacg aaacggcagc ccgcggcaac tggtcggacc
     2161 cgcaattcct ggcgaacctg accgacctca acagcgtgac gcggcacacg cagttcgccg
     2221 actttcacgg ccacggctgg gcgttccgcg cggttttcaa aaaagaccgc gcggggcacc
     2281 tgctcgactg gcagggacgg cgcgtcgccg cggtgacgaa cgacaagctc cgcgaagcgg
     2341 tccgcccgcc gaccgaagac gagaagactg tcggcaagca gcgcgtgggc acgccggtcc
     2401 atttgatgga tattcacctg gaaaaaggca tgcactgcgt cgattgccat ttccagcagg
     2461 acaatcacgg caacacgaag ctctacggcg aagtgcgggc ggcgattgaa atccagtgca
     2521 tcgattgcca cggcacggct ggcgagacgg cgccggaacg gatcgagcga accggccgcc
     2581 tcgcgacgag cggcccggcc gcgccccccg gcggcacgaa cctgctcacg ctccgcacgc
     2641 cgcacactcc gcggcaggcc cgattcgaat tgcagcccga cgcgccgacc tccgacggca
     2701 agccgcggta caaactgatc cagcgttcga tggtcgagcc agatcgaaag tgggaggtca
     2761 aacagaccgc gtacacaatc cgccccgacc atcccgacta caacgaaaag tcgcacgcgg
     2821 cgaagaccgt gcgctgggac gcggcgggcg aattgctctg gggtgggcag cccgatcccc
     2881 aggaggagat cggcgggcga aagtgtgccc atcagagcaa caccatcagt tgcatcgcct
     2941 gccattcctc gtggaatccc agctgtttcg gctgccacct gccccagaag gcgaaccaga
     3001 agaccccgag cctgcacaac gagggcgacg tctcccgcaa cctggtgcgg tacaactttc
     3061 agacgctccg cgacgacgtg ttcatgctcg cccgcgatgg gctggccacc ggcaaccgca
     3121 tcggcccggc ccgatcgtcc tgcgcggtcc atgtcagctc gtacaacgcc aatcgcgaaa
     3181 atgtctatca ccagcagcag acgatcagcg gctgcgggct gtcgggcatc gcgttcagca
     3241 ccaacgtgcc gcacaccgtg cgcggcggca gccgcagcct gctgaatccc gccccgggca
     3301 cgagcgagac gaaatcgtgc agcgactgcc acccttcgaa gaacaacgac aacaacgcgc
     3361 tgctcgctca gctgctgatg cagggcacga actacacgaa tttcatgggc aaatactgct
     3421 gggtcgcggc gggcgagcac gggctggagg ccgtcgtcgt cacggaacag gacgagccgc
     3481 aagccgtgat cggcagcacg ctgcaccgcg tggcgtaccc cgagcggcat cggcaccacc
     3541 tggagcgggg cggcgagctg gagcacgccc acgagcatcc cggcgtggac attctcgacc
     3601 agctccgcca tccgctgcaa aagccggaga ttctgcaggt gcaggcccgc ggcgaattcc
     3661 tgtacgcggc ctgcggcagc ggcggattcc gcgtcttcga catggcgttt atcgatcaca
     3721 aggggttctc cgagcgcatc accactgcac cggtctcacc gctgggccag cagctctacg
     3781 tcccgacgaa gtacgccacg gccgttgccg cgccgacgac gctcgcgccg gacccgacgc
     3841 ggcagcaact gcccgagaat cgcgagcaat ccgttcatgc gctttacgcc tacatctatg
     3901 tcgccgacaa gtacgagggc ctgatcctgg tcaatgcggc gactctcctg gacggaaatc
     3961 cgctcaacaa cttcttgtcg cgatcgctga cctacaaccc cggcggcttg ctcgccggag
     4021 cacgccacat cacgatcgcc ggcacgttcg cctacgtctc gtgcgacgcg gggctggtcg
     4081 tgatcgcgtt ggacgatccg gtcaatccgt atgtcacggc agtcatcgac gccgaaacgc
     4141 tgccgcaggc cggcgtcagc caggtgcagt ttcgctatgc gttcgtctgc gccgcggacg
     4201 gattgcacgt gctcgacgtg accgatctgg cccagccgaa accggcgggt cacttgccgc
     4261 tggccgattg ccgcagcgtc tatgtggccc ggacctacgc ctacatcgcg gccggtcacc
     4321 agggtctggt catcgccgac atcacgcggc ccgaccgccc ggcgatcgac cagatcttcg
     4381 atgctggcgg ctgcatcgac gacctgcacg acgtcaagct cggcatcacc tacaccagcc
     4441 agttcgccta cctggccgac ggccagaacg ggctccgcgt cgtgcagctc accagccccg
     4501 acacgcccgg caattccggt ttcagcccac ggccgacgcc gcagctcgtg gcgacgcgga
     4561 agctgccgcg cggcggccac gcactgttcg tctccgaggc cctcgaccgc gaccgcgccg
     4621 tcgatgaatc cggcaaccag atcgccgtct tcggccgcat cggcgctcgc ccgctgttgc
     4681 gcgaggagca gcagcggatg tacctgcggc ccgccacgga cggcaagggc ccacgccggc
     4741 cctggtgggt cagcgacaac gtgtttgacc gcgaag
//
LOCUS       DASRJB010000645         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3572224, whole genome shotgun sequence.
ACCESSION   DASRJB010000645 DASRJB010000000
VERSION     DASRJB010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572224"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFB80_17090"
     CDS             <1..784
                     /locus_tag="VFB80_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35549.1"
                     /translation="GAQEIVAHAPVPKHCIPNGTLDPDRLIFYGGTAAGEGADGEGVQ
                     FFAYDCKSRKLIYSGPDGPARYMILARSTGRVYFVPGKGDSPLMRFDPATDKAPVKIE
                     GEIGIRAATRETPQGIVYTVSLGQGAGAVASIYAFNTKTEKIEVLGPAAVATNNYVAA
                     VSADPTGRYLYYVPGAHGGSERDGSAVIQYDTKTRKPKVIAFLHPYFEKKYGFVPKGT
                     YAVACDPAGDKLYITWNVSRGSRAWDCCGVTAIHIPASERPA"
     gene            complement(781..2706)
                     /locus_tag="VFB80_17095"
     CDS             complement(781..2706)
                     /locus_tag="VFB80_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZN35550.1"
                     /translation="MIYETSWTAIGVFAAFVALVLGISFYLGAKAKSASGYYAAHGQI
                     PWFVNGVAFAGDYLSAASFLGICGMIAFYGYDGFLYSIGYLAGWIVALLVIAEPLKRL
                     GKFTFADALDSQFNSRGIKLAAAISTLVVSVFYLIPQMVGAGALVKPLLGFPEHYQGV
                     VTVGLIVILIVVTAGMVSTTWVQFIKGGMLVVFCAILTAMIVGRGLTALEAPPREMAE
                     GAKVLSPGGDWAAKKGADFRQVEVAPGQVRVERRYGDDWRLVQSSFKHHGQTMVNGLP
                     QGKKPGQTDFAPVGRIAELPAGRRQTGPLNPLSYLGTLQQSQVEVWRSLKLKDERAVE
                     TTIYYPLVVRGDELLTPGGSPTFSALRTNLYVEREGQQVLSWSAIWSKLDFLSLMIAL
                     FGGTASLPHILIRYYTVKDQASARKSTVVGIASIGFFYVLTLFLGLGAMTSGRLDVTD
                     NNMAAPLLARSFGELPFAVVSAIAFTTVLGTVSGLIMAASGAVAHDLLANSLRLGLSD
                     YAKVRAGKITAVFVGLVAIALGILFGRLNVNFLVGWAFNIAASANLPALVMLLFWRGT
                     TRQGITAAIFVGMLSSLAWILLSQQCYRDVYGLPPESALVPFSQPGIVTIPLGFAVLV
                     AVSLFTRGNNGRPGGRG"
     gene            complement(2703..3011)
                     /locus_tag="VFB80_17100"
     CDS             complement(2703..3011)
                     /locus_tag="VFB80_17100"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HZN35551.1"
                     /translation="MKAESPDKPAGGGAPHVDDNQAVSAANARSGLVLFFVYLAFYAG
                     FMGLATFAPGAMGQPVLAGVNLAIVYGMGLIAGALVVAAIYMWLCGRHARRYHAEAGR
                     "
     gene            complement(3062..3652)
                     /locus_tag="VFB80_17105"
     CDS             complement(3062..3652)
                     /locus_tag="VFB80_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35552.1"
                     /translation="MESQVFGEMSWERMIRAVENVRRRLIRVAQFLNAAGIPYAAAGG
                     NAVAAWVSTVDEAAVRNTQDVDIVLRRSDLPAAIAALEAAGFVHRHAAGIDMFLDGPT
                     AKARDAVHVVFAGEKVRPGYVEPAPQPDNPYVKDDVKFLRLEPLVRMKLTSNRRKDQV
                     HIEDLHSVGLVDESWCQELPKELGARLKHILDTLDQ"
     gene            3741..4109
                     /locus_tag="VFB80_17110"
     CDS             3741..4109
                     /locus_tag="VFB80_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35553.1"
                     /translation="MLRFQISIVGGAAGAPLAASFDSALAALEQLPRMFIEPDGSFVW
                     RGTTGDGQAWQVDGNLIDRGDVLAYVDASGHCPEEQFNELLRALGWPGAPLAFELTRR
                     GVMLDEQAFRKLARTPAGAV"
     gene            complement(4106..>5239)
                     /locus_tag="VFB80_17115"
     CDS             complement(4106..>5239)
                     /locus_tag="VFB80_17115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN35554.1"
                     /translation="LGPLMSVTPLANIVLLARDVLKGGADPLWGTVAVLSTLLYAGLA
                     LALAARIFGSDAILYGSQGSWSDLFRRPLEPREQATIPGALAALAIVGPLFVIAGGIL
                     AQLHAIAMQAQLAALAGTSFLLFIVLPLGLTRLQGVAVRSGFQLNAPKPLTLVGAAVL
                     GCCLWTVAYDLIILCQDLGIATLSLKKLEEARPGLEALFDRWRALPLAFVILAFAVTP
                     AVAEEFFFRGYLLGALRGRLPAWSAIGLTAVIFGLFHASVGGIIAVERVLASTLLGVF
                     LGWVAWTSRSIWPGVVLHALNNSLMLLIARYANELKARGWDSEQQRYLPNLVLAVAVV
                     GIVVGAAIIWRSRIQEPAAKATLKEQKPNLPAAADPPAAGATL"
BASE COUNT          997 a         1703 c         1712 g          827 t
ORIGIN      
        1 tggagcccag gagatcgttg ctcacgcccc cgtacccaag cactgcatcc ccaacggcac
       61 gctcgacccc gatcggctga tcttttacgg cgggacggcg gcgggcgaag gggccgacgg
      121 cgaaggggtg cagttcttcg cctacgattg caagagccgc aagctgatct attccggccc
      181 ggacggcccg gcccggtaca tgatcctggc ccgctcgaca ggccgcgtct atttcgtgcc
      241 aggcaagggc gattcgccgc tgatgcggtt cgacccggcg accgacaaag ccccggtgaa
      301 gatcgagggg gagatcggca tccgggccgc gacccgcgaa acgccgcagg gcatcgttta
      361 cacggtttcg ctcggacagg gcgcgggcgc tgtcgcctcg atctatgcct tcaacaccaa
      421 gaccgagaag atcgaggtgc tcgggcccgc tgcggtcgcc acgaacaact acgtggctgc
      481 ggtcagcgcc gatcccaccg gccgctacct ctattatgtg cccggcgcac acggcggcag
      541 cgagcgcgac ggctcggccg taattcaata cgacacgaag acccgcaaac ccaaggttat
      601 cgcgttcttg catccctact tcgagaagaa gtacggcttc gttcccaagg ggacctatgc
      661 cgtggcctgc gacccggccg gcgacaagct ctacatcacc tggaacgtca gccgcggctc
      721 gcgggcctgg gactgctgcg gcgtgacggc gatccatatt ccagccagcg aacggcccgc
      781 ttagccccgg cctccaggcc ggccattgtt gccgcgggtg aacagcgaca cggcgaccag
      841 caccgcaaag cccagtggga tcgtcacgat cccgggctgg ctgaagggca cgagcgcgga
      901 ttccggcggc aggccataga cgtcgcggta gcactgctgc gagagcagaa tccaggccag
      961 cgacgagagc atgccgacga agatcgcggc cgtgatgccc tgccgggtcg tgccgcgcca
     1021 gaagaggagc atcaccagcg ccggcagatt ggcgctggcg gcgatgttga aggcccagcc
     1081 gacgaggaag ttgacgttga ggcggccgaa gaggattccc agcgcgatgg cgaccagtcc
     1141 cacgaaaacg gcggttattt tgccggcgcg gaccttggca taatcgctca ggcccagccg
     1201 cagggaattc gccagcaagt cgtgggcgac agcgccgctg gcggccatga tcaggccgct
     1261 caccgttccc aggacggtcg tgaaggcgat cgccgagacc acggcgaacg gcagctcgcc
     1321 gaagctgcgg gctagtagcg gcgcggccat gttgttgtcg gtcacgtcga ggcgtccgct
     1381 ggtcatggcc cccaggccca gaaacagcgt caggacataa aagaagccga tgctggcgat
     1441 gccgacgacg gtgctcttgc gggcggaagc ctggtccttg accgtgtaat aacggatcaa
     1501 aatgtgcggc agcgaagcag tcccgccgaa cagagcgatc atcagcgaca ggaagtccag
     1561 cttcgaccag atggccgacc agctcaacac ctgctggcct tcccgttcga catacaggtt
     1621 cgtccgcagg gcactaaagg tggggctgcc gccgggcgtg agcaactcgt cgccgcgcac
     1681 gaccagggga tagtagatgg tcgtctccac ggcccgctcg tccttgagct tgaggctgcg
     1741 ccacacttcc acctgactct gctgcaacgt gcctagatag gacagcggat tgagtggtcc
     1801 agtctgccgg cgaccggccg gcaactccgc gatgcggccg acgggagcaa agtcggtctg
     1861 tccgggtttt ttgccttgcg gcaggccgtt caccatcgtc tggccgtggt gcttgaacga
     1921 ggactgaacc agtcgccagt cgtcgccgta cctccgctcg actcgcacct gtccgggagc
     1981 gacttcgact tgccggaaat cggcgccttt tttggcggcc cagtcgccgc ctggcgatag
     2041 cactttcgca ccctctgcca tctcccgcgg aggagcttca agcgcggtca gtccgcggcc
     2101 gacgatcatc gcggtcagaa tcgcgcagaa cacgacgagc attccgcctt tgatgaactg
     2161 cacccaggtc gtagagacca tgccggcggt caccacgatc aatatcacaa tcaggcccac
     2221 cgtcaccacg ccttgatagt gctcgggaaa gcccaagagc ggcttgacca gcgcccccgc
     2281 acccaccatc tgcggaatca agtaaaacac gctcacgacg agcgtgctga tcgccgcggc
     2341 gagcttgatg ccgcggctgt tgaactggct gtcgagggcg tcggcgaacg tgaatttgcc
     2401 tagccgcttg agcggctcgg cgatgaccag gagggcgacg atccagccgg cgaggtagcc
     2461 gatggaatag aggaagccgt cgtagccgta gaaggcgatc atcccgcaga tgccgaggaa
     2521 cgacgcggcc gagaggtagt cgccggcgaa ggcgacgccg ttgacgaacc agggaatctg
     2581 cccgtgggcc gcgtagtagc cgctggcgct cttggccttg gcgcccaggt agaagctgat
     2641 gcccaggacg agggcgacga acgcggcaaa gacgccgatc gccgtccagg aggtttcgta
     2701 gatcaccggc cggcctccgc gtgatagcgg cgggcatggc ggccgcagag ccacatatag
     2761 atcgccgcga cgacgagcgc tcccgcgatc agccccatgc catagacaat cgccaggttc
     2821 acgcccgcca gcacaggctg gcccatcgct cccggcgcga acgtcgccag ccccatgaat
     2881 cccgcgtaaa acgccaggta aacgaaaaac aggaccagcc ccgagcgggc attggcggcg
     2941 ctgaccgcct ggttgtcatc gacgtgcggc gcgccgccgc ctgccggctt gtcgggagat
     3001 tctgccttca taggccgcca gaataacagc ggcccccgcg gctgtcacgc gagcgacctg
     3061 atcattgatc cagcgtgtcc aggatgtgct taaggcgggc accgagttcc ttcggcaatt
     3121 cctggcacca gctttcatcg acgagtccaa cgctgtgcag atcctcgata tggacctgat
     3181 ccttgcgacg attcgacgtg agcttcatcc gcaccagggg ctccaaacgc aggaatttta
     3241 catcgtcctt tacatacgga ttgtccggct gtggcgccgg ctcgacataa ccaggccgca
     3301 ctttctcgcc ggcgaacacg acatgcacgg cgtcgcgagc cttggcggtc gggccgtcga
     3361 ggaacatgtc gattcccgcg gcatggcggt gtacgaagcc ggccgcttcg agcgccgcga
     3421 tggccgccgg cagatcgctg cggcgcagga cgatgtccac gtcctgggtg ttgcggacgg
     3481 cggcctcatc gaccgtcgaa acccaggcgg ccacggcatt accgccggcc gcggcgtacg
     3541 ggattcccgc ggcgttcagg aattgagcca cgcgaatcaa tcggcggcgc acgttttcca
     3601 cggcacggat catcctttcc caggacattt cgccaaagac ttgcgattcc atgggccgtc
     3661 aactcgaagg cagcgcaggc acaccttctg ttacgataac ctccgtcgga tgagccgcaa
     3721 actgcgaatt tcttcggacc atgttgcggt ttcagatttc cattgtcggc ggcgcagcgg
     3781 gcgccccgct ggccgctagt ttcgactctg cgctcgccgc actggagcaa ctgccccgga
     3841 tgttcattga gcccgacggc tcgttcgttt ggcgaggcac gacaggcgac ggacaagcgt
     3901 ggcaagtcga tggaaacctg atcgatcggg gcgacgtgct ggcgtatgtc gatgccagcg
     3961 gccactgccc ggaggagcaa ttcaacgagt tgctgcgagc cctgggctgg ccaggtgcac
     4021 cgctcgcgtt cgagctgacg cggcggggcg tgatgctgga tgagcaggcg tttcgaaagc
     4081 tggcgcggac gcccgccggc gcagtctaaa gcgtcgcgcc ggcggcggga ggatcggctg
     4141 cggccggcag gttcggtttc tgttctttga gcgtggcttt ggccgccggt tcctggatac
     4201 ggctccgcca aatgatcgcc gcccccacga caatgccaac gaccgctacc gccaggacca
     4261 gattcggcag gtatctctgc tgctccgagt cccagccgcg ggccttgagt tcgttggcgt
     4321 agcgggcgat gaggagcatc aggctgttgt tcagcgcgtg cagcacgact ccgggccaga
     4381 tgctgcggct ggtccacgcg acccagccga gaaacacgcc gaggagggtg ctggccagca
     4441 cgcgctcgac ggcgatgatt ccgccgacgc tggcgtgaaa cagaccaaag atgacggcgg
     4501 tcaagccaat ggccgaccac gcgggcaggc ggccgcgcag tgcgccgagc agatagcccc
     4561 ggaagaaaaa ctcctcggcg accgcgggtg ttacggcgaa ggcgagaatc acaaaggcca
     4621 ggggcaacgc ccgccagcga tcgaagaggg cctccaatcc tggccgcgct tcctcgagct
     4681 tcttcaaact cagcgtggcg atccccaagt cctggcagag gatgatcagg tcgtaggcca
     4741 cggtccacag gcagcagccc aaaacggctg cgcccaccag tgtcagcggc ttgggtgcat
     4801 tcaactgaaa cccgctgcgc acggccacgc cctgcaagcg ggtcagtccc agcggcagca
     4861 cgatgaacag caggaaggaa gtgccggcga gtgcagccag ctgagcctgc atggcgatgg
     4921 cgtgcagctg tgccaggatg ccgcctgcga ttacaaagag cggcccaacg atggccagcg
     4981 cggcgagtgc accgggtatt gtggcctgct cgcgcggctc cagcgggcgg cggaacaggt
     5041 cgctccaact gccctggctg ccgtacagga ttgcgtcgct gccgaagatt cgagcggcca
     5101 gcgccagcgc gagcccggcg tagagcagcg tcgaaagcac cgccaccgtg ccccacagcg
     5161 ggtcggcccc gcccttcagc acgtcgcggg ccaggagcac gatgttggcc aagggcgtga
     5221 cgctcatcag cgggccgag
//
LOCUS       DASRJB010000646         8011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2445305, whole genome shotgun sequence.
ACCESSION   DASRJB010000646 DASRJB010000000
VERSION     DASRJB010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445305"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="VFB80_17120"
     CDS             <1..2297
                     /locus_tag="VFB80_17120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN35555.1"
                     /translation="SEFLASEDSWFRPSMCRTGPDGALWISDMYRFVIEHPKWIPAAA
                     QAKVDMRAGDDKGRIYRVYPTGKKPRPIARLDKLDTPGLVAALDSPNGQERDLAQQML
                     IWRGDKDATLPLIKLAAESKRPLARLHALCTLEGLGTLSSDVLLKALGDPDAGVRRHA
                     VRLGESRLSDSPKLRDALTGLLDDKDPQVRLQVAYSLGAAADARVAVLLLTNTADPYV
                     TAAALSSVQKQNISEVLELALSGATPSADSTRLIERLLTMAAAMGEDAAVGRAAAALL
                     KPQSNASYAPWQFTSLASVADALARRNVKLASVLDEGSGEQLRRLLAQARSAVANVNG
                     PPLPAAVAATALLARGLDDQDAADFELLAKLLAPQRPPELKSAAVAALARSGGDAPKV
                     LLAGWAAHSPALRSQILDALTARQDWSLVVLDAIEQGQIPAAQLDARRRQQFLTSRNR
                     SVRERAEKVLAGAIDPNRQKLVATYLASATGGDAPRGKVVYAKRCANCHKLEGAGHTV
                     GPDLAALNNRSPEVLLTAILDPNRAVEDRYMEYVVLTTDGRQANGMLLEETGASLTLV
                     APEGKQTVIPRGELDQLKSSGKSLMPEGVEKDITPAEMADLVAYLRSSTPPPKKLAGN
                     APEIVTPFVDGSIRLLATTARAYGPSLVLEEKYRNFGYWHSPEDFCAWTFEVPAGAGG
                     EYRLSLDYACEENAAGNTVEVQVGGQTFSAKVPGTGTWDDYRGLNLGTVTLAAGQGEL
                     LVRSAGPIKTALLDLRGVRLVPVK"
     gene            complement(2337..3005)
                     /locus_tag="VFB80_17125"
     CDS             complement(2337..3005)
                     /locus_tag="VFB80_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN35556.1"
                     /translation="MPVIDSIQVGQPTDYLHEGYADGKQRSWTTAFFKTPVAGSVHVG
                     RLGVSGDRQADLHNHGGVDKAVLAYSADHYAYWRQHLALPEMPPGGFGENLSIAGLDE
                     AGVSIGDRWQAGTVLFEVTQPRQPCWKMSRRWQLPDLAAQVVANGRSGWYLRVLAEGE
                     LAAGTELTLVRRPHPAWTIARANRLMHHEKDNVSAAEKLAALSELSAAWKESLHKRIA
                     NRAL"
     gene            complement(3068..3325)
                     /locus_tag="VFB80_17130"
     CDS             complement(3068..3325)
                     /locus_tag="VFB80_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35557.1"
                     /translation="MPDITYAELFAFLSSLGFQEFSGSAFDAVWRHPASGAVVVFSLA
                     APHDAARGADLLSAEIRLQHHGLLEGSLRGAIVAARVHGET"
     gene            complement(3336..3608)
                     /locus_tag="VFB80_17135"
     CDS             complement(3336..3608)
                     /locus_tag="VFB80_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35558.1"
                     /translation="MAAAKKGEINLAQLRSFLLSLGFEQPISVNRSQAFRHRKSGTVL
                     VVSASGASGSLRPADLLSVLVRLESEGLARESDLRQLRLGKLPKAS"
     gene            complement(3586..4131)
                     /locus_tag="VFB80_17140"
     CDS             complement(3586..4131)
                     /locus_tag="VFB80_17140"
                     /inference="COORDINATES: protein motif:HMM:NF025638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HZN35559.1"
                     /translation="MGRESGRRRRKRRTREHVLEDLSENYLEHKVLLCGHILRRPERD
                     YGVDVTMFHFDGKGRIENGEVRFQLKATDRLITAKDGAVVTYPIKIGDLHYWSREFYP
                     FILVIFDAKATRAFWLNVQDYVHEHPGIDEGATDKVNVHVPVTNELTAASIKLFRKRS
                     LESIAQLTSQGGLSDGGRKKR"
     gene            complement(4281..5390)
                     /locus_tag="VFB80_17145"
     CDS             complement(4281..5390)
                     /locus_tag="VFB80_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35560.1"
                     /translation="MTANTQRRSFLGAVGAGTAIAITPSFLRAAPFVPARDPQVVAFA
                     PGGTLIATGCSGMTDGTFPPRPHPDVRKCGVVAIWDIASRKRLFRWETFGDLTKLAFS
                     PDGTLLAACRLFATDDGVELNEVRVWNVVSGQLVKALDRCHSFDFSPDGRQLAVLSRS
                     KCALYDVRDWAKERLVQPLSGAIGVAFSADGLSLIGIVKDQERYRLRLASIDSNQTVA
                     AEALQSLPLDAPYYRVSVAADGTLLATGHDGGNVVVWDAATLDVKSRFQTGIRGLAHP
                     FFGPDVKHLAAGCQENGDVLVWSLADRQELAHHTFAKGALRTYYTRPESAVFRPEKDP
                     SRFCFSPDGGSFLAGCYGGILRATLTGQELARFGD"
     gene            5503..6684
                     /locus_tag="VFB80_17150"
     CDS             5503..6684
                     /locus_tag="VFB80_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35561.1"
                     /translation="MPRPSDLCPRPQPLPPLATQPHAPPIYPASVWACTSPQQADDLL
                     GGREAGYVYQRDGHPNADLLSEKLRQLHAADRGAITASGMGALAAALLSRLEAGDHAV
                     IGSRVYGKTLTLFGGEASRLAIETTAVDTCDLKAVEAAIRPTTRLLLAETIANPLLEV
                     ADVAALAEIAHRKNTALLIDNTFASPILCRPLDLGADLVMESLTKTLNGHSDVVLGFL
                     GGREDVWKRVPGVISAWGLVSSPFDCWLAERGLATAHLRIERACQNALAAAQFLAGQQ
                     PKVAEVHYPGLSSHPQHTLAQRQFGGLFGTIVSFRLAGGRAAADAFIDTAKQIPFCPS
                     LGELSTTLSHPETTSHRGLTPEQRRLLGISGGTIRLSVGTESLEFIRAALAQGLQGLE
                     R"
     gene            6882..>8011
                     /locus_tag="VFB80_17155"
     CDS             6882..>8011
                     /locus_tag="VFB80_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN35562.1"
                     /translation="MRRLLAITIVFAFAARAQAQQPAAYDLVVYGGTAGGVVAAVQAR
                     RLGKTAVLIEPSQHLGGLTSGGLGATDIGNKAAIGGISREFYQRVRKHYSEPANWKYE
                     KPEQYRSGRGSEVGQEDAMWTFEPSVAEKILDEMVKEAGVVVVKGQRLDLQKGVVKNG
                     GRIASIRMESGQTFAGKMFVDATYEGDLMAKAGVSYTVGREGNSQYGETLNGVQTKNA
                     TKHQFVAGVDPYVKKGDKSSGLLPRVHAGPPGEEGSADKRVQAYNFRMCLTDVKANQI
                     PFAKPAGYDALQYELLLRNFEAGETRAPWNPILMPNRKTDVNNNFGFSSDNIGMNYDW
                     PDADYATREKLFAEHLAYQQGLLWTLVSNPRVPEKIRQEVSR"
BASE COUNT         1437 a         2674 c         2635 g         1265 t
ORIGIN      
        1 ccagcgagtt cctggcctcg gaagacagct ggttccgccc cagcatgtgc cgcaccggcc
       61 ccgacggcgc actgtggatc agcgacatgt accgcttcgt gatcgagcac cccaagtgga
      121 ttcccgcagc agctcaggcg aaagtcgata tgcgggccgg cgacgacaag ggccgcatct
      181 accgcgtcta tcccacgggc aagaagcccc gccccatcgc ccgcctcgac aagctggata
      241 ctcccggcct cgtcgccgcc ctcgattccc ccaacggcca agagcgcgat ctcgctcagc
      301 agatgctgat ctggcgcggc gacaaggacg cgacattgcc gctcataaaa ctggcggccg
      361 aatccaagcg gccgctggct cgcctgcacg cgctgtgcac gctggagggg ctgggcacgt
      421 tgtccagcga cgtgctgctc aaagccttgg gcgatccgga cgccggcgtc cgccggcatg
      481 ccgtccgcct gggcgaatcg cggctcagcg actcgccgaa actgcgcgac gccctgaccg
      541 ggctcctcga cgataaggac ccccaggtcc gcctgcaggt tgcctactcg ctcggcgccg
      601 ccgccgatgc acgcgtcgcg gtgctgcttc tgaccaatac ggccgaccct tatgtgactg
      661 ccgccgcgct cagctcggtc cagaagcaga acattagcga ggtgctggag ctggcgctct
      721 caggtgcgac gccaagcgcc gactccaccc ggctcatcga gcggctgctc accatggccg
      781 ccgcgatggg cgaggacgca gccgtcgggc gggccgcggc ggctctcctc aagccgcagt
      841 cgaatgccag ctatgccccg tggcaattca ccagcctggc tagcgtcgcc gacgcgctcg
      901 cgcggcggaa cgtcaaactg gcgagcgtgc tggacgaagg cagcggcgaa cagttgcggc
      961 ggctgctggc gcaggcccga tccgccgtgg caaatgtcaa cggtccgcca ttgcccgccg
     1021 cggttgccgc cacggccttg ctcgctcgcg gattggacga ccaggacgcg gccgacttcg
     1081 agctgctcgc gaagctactc gcgccgcagc ggccgccgga attgaaatca gccgcggtgg
     1141 ccgcgctggc ccgctcgggg ggcgatgcgc caaaggtgct cttggccggc tgggcggcgc
     1201 attcgccggc gctgcggagt cagattctcg acgcgctgac ggcacggcag gattggtcgc
     1261 tggtggtctt ggacgcgatt gaacaaggcc agatccccgc ggcgcagctc gatgcccggc
     1321 ggcggcagca gttcttgacc agccgcaatc gcagtgtgcg cgagcgggcg gaaaaagtac
     1381 tggccggggc gatcgatccc aaccggcaga agctggtcgc gacttacctg gcctctgcca
     1441 ctggcggcga cgcgccgcgc ggcaaggtcg tgtacgcgaa gcgctgcgcg aattgccaca
     1501 agctggaagg cgcggggcat acggtcggcc cggatctggc ggcgctcaac aaccgctcgc
     1561 cggaagtgct gctgacggcg attctcgacc ccaatcgcgc ggtcgaggac cgctacatgg
     1621 aatacgtcgt gctcacgacc gacggtcggc aggccaacgg catgctgctc gaagaaaccg
     1681 gcgcgagcct gactctggtt gcgcccgaag gaaagcagac ggtgattccc cgcggcgaat
     1741 tggaccagct caagtccagc ggcaagtcgc tgatgcccga aggggtcgag aaggacatca
     1801 cgcccgcgga gatggccgac ctggtggcct atctccgcag cagcacgccg ccgcccaaga
     1861 agctggcggg gaacgcgccc gaaatcgtca cgccattcgt ggatggctcg attcgcctcc
     1921 tggcgacgac ggcccgggcc tatgggcctt cgctcgtgct ggaggagaag taccgcaatt
     1981 ttggctactg gcacagcccg gaggatttct gcgcctggac gttcgaggtg ccggcgggcg
     2041 cggggggcga gtaccgcctg tcgctcgact acgcctgcga ggagaacgcc gccggcaata
     2101 cggtcgaggt ccaagtgggc gggcagacct tcagcgcgaa agtccccggc acgggcacgt
     2161 gggacgacta ccggggcctc aacctgggca cggtcacgct cgccgccggc cagggcgaat
     2221 tgctcgtccg ctccgccggc ccgatcaaga ccgcattgct cgacctccgc ggcgtgcggc
     2281 tggttccagt gaagtagggt ggaccaacga gtccgcgagc gccggcccac cacgaattac
     2341 aaagcgcgat ttgcaatccg cttgtgcagc gactccttcc acgccgccga gagctcgctt
     2401 agcgcggcga gtttctcggc cgcactgacg ttgtctttct cgtggtgcat cagccggttg
     2461 gcgcgggcga tggtccaggc cggatgcggc cggcggacaa gcgtcagctc cgtaccagcg
     2521 gccagctcgc cctccgccag cacacgcaga taccagccgc tgcggccgtt ggcgacgact
     2581 tgggccgcga gatccggaag ctgccagcgg cgggacattt tccagcaggg ctgccgcggc
     2641 tgcgtaacct cgaagagcac cgtgcccgcc tgccagcggt cgccgatcga gacgcctgct
     2701 tcgtcgagcc cggcaatcga gaggttctcg ccaaacccgc ccggcggcat ctcgggcaag
     2761 gcgagatgct gccgccaata cgcatagtga tcggccgagt aggccagcac cgccttatcg
     2821 acgccgccgt ggttgtgcag atccgcctgc cggtcgccgc tcacaccgag gcggccgacg
     2881 tgcacgctcc ccgcgaccgg cgtcttgaag aacgccgtcg tccacgagcg ctgcttgccg
     2941 tcggcataac cttcgtggag atagtcggtg ggctggccga cttggatgga atcaatgact
     3001 ggcatcccct atttttcgtc cgtgacggcg tgggccacaa ccagagcggg ccgagccggg
     3061 gcgatggcta cgtctcacca tgaactcttg cagccacgat ggcgccccgc agggatcctt
     3121 ccaagaggcc gtgatgctgc aatcttattt cggcggacaa caggtccgcg ccgcgggcgg
     3181 catcatgggg cgcggccaat gaaaagacga cgacggcgcc gctcgcggga tgcctccaga
     3241 cagcgtcaaa agccgatccc gagaattcct ggaagcccag gctgctcagg aatgcgaaca
     3301 gctcagcgta ggtgatgtct ggcattcgca aggtcctacg acgccttggg cagtttgccc
     3361 aggcgcagct gccgcaagtc ggattctctg gccagccctt cgctttccag tcgcacgagc
     3421 accgagagca gatcggccgg ccggaggctg ccactagcgc ccgatgcgga cacgaccagc
     3481 accgtgcccg acttgcggtg acgaaaggcc tgcgagcgat tgacgcttat tggctgctca
     3541 aagcccaggg aaagcaggaa actccgcagt tgtgcaaggt tgatttcacc tttttttgcg
     3601 gccgccatcg gagagtcctc cttgactggt caactgcgcg atcgattcca gcgaccgctt
     3661 gcgaaacagt ttgatggatg cagcggtcaa ttcattcgtc accgggacgt ggacattcac
     3721 cttatccgtc gcgccttcat cgatgccggg gtgctcgtgc acgtagtctt gcacgttgag
     3781 ccagaacgct cgcgtcgctt tggcgtcgaa gataacgagg atgaagggat aaaactcgcg
     3841 cgaccaataa tggagatcac cgattttgat gggataagta acaaccgcgc catccttggc
     3901 ggtaatcaat ctatcagtcg ccttgagttg aaaccggact tcgccatttt cgatccgtcc
     3961 ctttccatcg aagtgaaaca tggtaacgtc cacaccatag tcccgctccg gccggcggag
     4021 aatgtgaccg cacagcaaca ccttgtgctc taaatagttt tcgctgaggt cttccaggac
     4081 atgctcgcga gtacgacgct tgcgtcgccg ccgtcccgac tctcggccca tcgacaatgc
     4141 tcgaatgcgc agaaaatgtt agtcgaaagc aggccacgat tgtaggttga cctgcccgcc
     4201 gctgctagca ttgcggcgct ggcttgcaag cattgtcaag cgcgagaatt aagggcaggc
     4261 cggaacgccg cctcacacgt tcagtcgccg aagcgggcga gttcctggcc ggtgagcgtg
     4321 gcgcggagaa tgccgccgta gcagccggcg aggaacgacc cgccatctgg cgagaaacag
     4381 aatcgcgagg ggtccttttc cgggcggaac acggcgctct cggggcgcgt gtagtacgtg
     4441 cgcagcgcgc ccttcgcgaa cgtgtggtgg gccaactcct gccggtcggc gaggctccac
     4501 accagcacgt cgccgttctc ctggcagccg gcggccaggt gcttaacgtc ggggccgaag
     4561 aacggatggg ccaggccgcg gatgccggtc tggaagcgcg acttcacgtc cagcgtggcg
     4621 gcgtcccaga cgacgacgtt gccgccgtcg tgccccgtgg ccaggagcgt gccgtcggcg
     4681 gcgacgctga cgcgataata cggagcgtcc agcggcagcg actgtagcgc ctcggcggcc
     4741 accgtttgat tcgagtcgat gctcgccagc cgcaggcggt atctctcctg gtccttgaca
     4801 atgccgatca gcgacaggcc gtcggccgag aacgccacgc cgatcgcccc ggaaagcggc
     4861 tgcaccagcc gctccttggc ccagtcgcgg acgtcgtaca gggcacactt gctgcggctg
     4921 aggacggcga gctggcggcc gtcgggcgag aagtcgaagc tatggcagcg atcgagggcc
     4981 ttgaccagct gcccgctgac gacgttccag acgcggactt cgttcagttc cacgccgtcg
     5041 tcggtggcaa acagccggca ggcggccagc agcgtgccgt cgggcgagaa agcgagcttc
     5101 gtcagatcgc caaacgtctc ccagcggaag agccgcttgc gcgaagcgat gtcccagatc
     5161 gccacgacgc cgcacttgcg gacgtcgggg tgcggccgcg gcgggaacgt gccgtcggtc
     5221 atccccgagc agcccgtcgc gatcagcgtc ccgcccggcg cgaatgcgac cacctgcgga
     5281 tcgcgggcgg ggacgaacgg agcggcacga agaaaacttg gcgttatcgc aatcgccgtt
     5341 ccagcaccga ccgcacccag aaacgaccgc cgctgagtat tggctgtcat gaccacctcc
     5401 taccactcac cactcactac caccaacata ctccactcag ccgccccgcg caccctatac
     5461 tcagctttgt cgaaatccca aatccgaaat cccaaatccg caatgcctcg cccctccgat
     5521 ctctgtcccc gcccgcagcc gctgccgccg ctggcgacgc agccgcacgc cccgccgatc
     5581 tacccggcca gcgtttgggc ctgcaccagc ccgcagcaag ccgacgacct gctgggcggc
     5641 cgcgaggcgg gctacgtcta ccagcgcgac ggccatccca acgccgactt gctcagcgaa
     5701 aagctgcggc agctgcacgc ggccgatcgc ggggcgatca ccgcctccgg aatgggggcc
     5761 ctggccgcgg cgctcctgtc gcggctggag gccggcgacc acgccgtcat cggcagccgc
     5821 gtgtacggca aaacgctgac gctgtttggc ggcgaagcga gccggctggc gatcgagaca
     5881 acggctgtcg atacgtgcga tctgaaagcg gtcgaggccg cgatccgccc cacgacgcgg
     5941 ctgctcctgg ccgagacgat cgccaacccg ctcctggaag tggccgacgt ggccgcgctg
     6001 gccgagatcg cccaccgcaa gaacacggcg ctgctcatcg acaacacgtt tgcctcgccg
     6061 attctctgcc ggccgctgga cttgggcgcg gacctggtca tggagagcct gaccaagacg
     6121 ctcaacggac atagcgacgt ggtcctgggc tttctcggcg ggcgggaaga cgtctggaag
     6181 cgcgtgccgg gcgtgatttc cgcctggggc ctggtgagca gtcccttcga ctgctggctg
     6241 gccgagcgcg gcctggcgac ggcccatctg cgaatcgagc gagcgtgcca gaatgcgctg
     6301 gcggcggcgc agtttctcgc ggggcagcag ccgaaggtgg cggaggttca ctacccgggc
     6361 ctaagctcgc atccgcagca caccctggcc cagcggcagt tcggcggcct gttcggcacg
     6421 atcgtcagct tccgcctggc gggcggccga gcggcagccg acgccttcat cgacacggcc
     6481 aagcagattc cgttctgccc ttcgctgggc gagctctcga cgacgctcag ccatcccgaa
     6541 acgaccagcc accgcggcct gacgcccgag cagcgccggc tgctcggcat cagcggcggc
     6601 acgatccgcc tttcggtcgg caccgagtcg ctggagttca ttcgcgcggc gctcgcgcag
     6661 ggactgcagg gccttgagcg ctagtcgaat cctgagtggg gcgagatggc gttgctgagt
     6721 cccaagctcc gcttgggact ccactttttc gaagctctgc ttcgagatcg ggaagcggag
     6781 cttccgggct tgcgttccca agcggagctt gggaacgagg aaccggcgtg ggctacaatg
     6841 tgatccgccg gttcgacccc gttgcagatc gaggttttgc catgcgccgc ctattggcta
     6901 tcaccatcgt gttcgctttc gccgcgcggg cccaggccca gcagccggcg gcttacgacc
     6961 tggtcgtcta tggcggcacg gcgggcggcg tggtggcggc ggttcaggcg cggcggctgg
     7021 gcaaaacggc ggtgttaatc gagccttcgc agcacctggg cgggctgacc agcggcggcc
     7081 tgggcgcgac cgacatcggc aacaaggccg ccatcggcgg gatcagccgc gagttctatc
     7141 agcgggtccg gaagcactac tccgagccgg cgaactggaa gtacgagaag ccggagcaat
     7201 accgctcggg ccgcgggagc gaagtcggcc aggaagacgc gatgtggacc tttgagccca
     7261 gcgtcgccga gaagattctc gatgaaatgg tcaaggaggc cggcgtggtc gtcgtgaagg
     7321 gccagcgcct ggatttgcaa aaaggcgtcg taaagaacgg cggccggatc gcgagcatcc
     7381 gcatggaatc ggggcagact ttcgccggca agatgttcgt cgatgcgacg tacgagggcg
     7441 acctgatggc caaagcgggc gtctcgtaca ccgtcggccg cgagggcaac agccagtacg
     7501 gcgagacgct caatggcgtg cagacgaaga acgcgaccaa gcaccagttc gtggccggcg
     7561 ttgatccgta cgtgaagaag ggggacaaat cgagcggcct cttgccgcgc gtgcacgccg
     7621 ggccgccggg cgaggagggc tcggccgaca agcgggtcca ggcctacaac ttccgcatgt
     7681 gcctgaccga cgtgaaggcc aaccagattc cgttcgcgaa acccgccggc tacgacgcgc
     7741 tgcagtacga gctgctgctc cgcaacttcg aggccggcga gacccgcgcg ccctggaatc
     7801 cgatcctgat gcccaaccgc aaaaccgacg tgaacaacaa ctttggcttc tcgtcggaca
     7861 acatcggcat gaactacgac tggccggacg cggactatgc cacccgcgag aagctgtttg
     7921 ccgagcacct ggcctaccag cagggcctct tgtggacgct ggtcagcaat ccccgcgtgc
     7981 ccgagaaaat ccgccaggaa gtcagccgct g
//
LOCUS       DASRJB010000647         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_189039, whole genome shotgun sequence.
ACCESSION   DASRJB010000647 DASRJB010000000
VERSION     DASRJB010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189039"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFB80_17160"
     CDS             complement(<1..342)
                     /locus_tag="VFB80_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35563.1"
                     /translation="MQTKSFVCAAVLAALAGWGGSARAQMGPMGGPMAPYGVMGVGGH
                     GMMAGAMPVSATGSISQGPNCAAPGCCSGVSCGDDGGCCDDCGGWCHHISVFGEFLYL
                     RPRNAEIAYSVP"
     gene            complement(493..1395)
                     /gene="lptB"
                     /locus_tag="VFB80_17165"
     CDS             complement(493..1395)
                     /gene="lptB"
                     /locus_tag="VFB80_17165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZN35564.1"
                     /translation="MRSAEFRIPNLNVTEDHMPILEAQGLVKTYGSRRVVNGVSFAVE
                     RAEIVGLLGRNGAGKTTSFRMTCGMIEADQGRVTLNGIDVTAWPMYRRAKDGGMGYLA
                     QESSVFRKLSVEQNLLGIMELLNMPRATRLRRCDELLEQFGIAKLRRNKAGSLSGGER
                     RRLEIARCLVSDPEVILLDEPFTGIDPVTIQNIQEVICDLKARGISVLITDHQVRETL
                     QITDRSYVIHDGEVLCHGTSAEVLANPRARELYFGSAIEVGSPAAADRLRLDPPTEPR
                     RASIAPRRAASASLHDADTLSADD"
     gene            complement(1409..>2178)
                     /locus_tag="VFB80_17170"
     CDS             complement(1409..>2178)
                     /locus_tag="VFB80_17170"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HZN35565.1"
                     /translation="CVADVTSKQGEIALLLVRSGAKYICRINLADGQATLSILDPSGQ
                     ELFFDGKDDESAAEKRPVAKTSVKGPGSYRLRLSNCDHQVLLWVNGSVVEFDGPTTYP
                     SDDLLEPATSEQDPGDLAPAGVGAGQGASATIRGLRIYRDKYYIATPGGYLAADYEQY
                     PSEALISRILATLHDPAEWNNGILASRGKRDFKLQNDQFLPLGDNSPASYDSRYWPEN
                     YVERDLLIGKALLIYWPHTWNRPIPFTPNFRKMGPIR"
BASE COUNT          369 a          741 c          663 g          405 t
ORIGIN      
        1 cggcaccgag tacgcgatct cagcattccg cggccgcaga tacaggaact cgccaaagac
       61 actgatgtga tggcaccagc cgccgcaatc gtcgcagcac ccgccatcat cgccgcagct
      121 gactccgctg cagcaaccgg gcgctgcaca gttcggccct tgggatatcg atcccgtcgc
      181 gctgaccggc atcgccccgg ccatcattcc atgtccgccg acgcccatca cgccgtacgg
      241 cgccatcggc cctcccatcg gccccatctg ggctctggct gagccacccc agcccgccag
      301 cgcagccagc acggctgcgc atacgaacga cttcgtttgc atcgtagaat ccccctgtgt
      361 gacaatgccc gcaggtaggg atcgaccgct aaagccgcta aatcgcgact ttttggagta
      421 ttccgcaatc tttgcgcgcc ctgcccggac cctgcatctt tcccaggcct ccatccttcc
      481 cggcacggct gctcaatcgt cggccgagag cgtgtcggcg tcgtgcagcg aggcgctagc
      541 agcgcgcctg ggagctatcg aggcccggcg aggttcagtc ggcggatcga ggcgcagccg
      601 gtccgccgcg gccggcgagc cgacttcgat ggcgctgccg aaatagagtt cgcgggccct
      661 tggattcgcg agcacttccg cggacgtgcc gtggcaaagc acctcgccgt cgtgaatcac
      721 gtagctgcgg tcggtgatct gcagcgtttc acgaacctgg tggtcggtga tcaggaccga
      781 aatcccacgg gccttcaagt cgcaaatgac ctcctgaatg ttctggatcg tgaccgggtc
      841 gatgcccgtg aacggctcgt cgaggaggat cacctccgga tcgctgacca ggcagcgggc
      901 gatctccagc cgccggcgct cgccgccgga gagcgagccg gctttgttcc tgcgcagctt
      961 ggcgatgccg aactgctcca gcagctcatc gcagcggcgg aggcgcgtgg cccgcggcat
     1021 gttcagcagc tccatgatcc ccagcaggtt ctgctcgacc gagagcttgc ggaagacgct
     1081 cgactcctgg gccaggtagc ccatgccgcc gtccttggcc cggcggtaca tcggccaggc
     1141 cgtgacgtcg atgccgttga gcgtcacgcg cccctggtcg gcctcgatca tcccgcaggt
     1201 catgcggaag ctggtcgtct tgcccgcgcc gttgcgccct agcagcccga cgatctccgc
     1261 tctctccacc gcaaagctga ctccattcac cacgcggcgg ctgccatagg tcttgacaag
     1321 cccttgggct tcgagaatcg gcatgtggtc ctccgtgacg ttcaaattcg gaatgcggaa
     1381 ttccgcactc cgcattccgc actccgcatt accggatagg tcccatcttc ctgaaattgg
     1441 gcgtaaacgg gatcgggcgg ttccaggtgt gcggccagta aatgagcagc gccttgccga
     1501 tcagcaggtc gcgctcgaca tagttctcgg gccaataccg gctgtcgtag ctggccgggc
     1561 tgttatcgcc cagcggcaga aattgatcgt tctggagctt gaagtcccgc ttgccgcggc
     1621 tcgcaaggat tccgttgttc cactcggccg gatcgtgcag cgtcgccaga atgcggctga
     1681 tgagggcttc actgggatac tgctcataat cggcggcgag gtaacctccc ggcgtcgcga
     1741 tgtaatactt gtcgcgatag attcgcaggc cgcggatcgt ggcggacgca ccctggcccg
     1801 cgccgacgcc cgccggcgca aggtcgcccg gatcctgctc tgaagtggct ggctccagca
     1861 aatcgtcaga gggataagtc gtgggcccgt cgaactccac aacggagccg ttgacccaga
     1921 gcaacacctg gtgatcgcag ttcgaaagtc gcaggcgata gctgcccggg cctttcacgc
     1981 tggttttcgc caccggccgc ttctcggcag ccgactcatc gtccttgccg tcgaagaaca
     2041 gctcctggcc gctcggatcg aggatcgaga gcgtggcctg gccgtcggcc aagttgatcc
     2101 ggcagatgta cttcgcaccg ctgcgtacga gcagcagcgc gatttccccc tgcttgctcg
     2161 tcacatcggc gacgcact
//
LOCUS       DASRJB010000648        12473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3291494, whole genome shotgun sequence.
ACCESSION   DASRJB010000648 DASRJB010000000
VERSION     DASRJB010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291494"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1244)
                     /locus_tag="VFB80_17175"
     CDS             complement(219..1244)
                     /locus_tag="VFB80_17175"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZN35566.1"
                     /translation="MKIGIVGYQGSGKSTLFHWLTGISPDPSLAHTIQSAMATIPDPR
                     VAQLCKIYQPKKVTQAALEIVDTPGLARTHEGSAQKLAMIREAGALVIVVAAFGGGNA
                     AADLRNFDDDLLIADLDILSGRVERLKDQMRKPRANKEKDQEELDLLTPLLAELEAGK
                     PLHALAMSPEQRRATRSFQLFSEKPRLIIVNTADDEDQPQRFASLAPAGSELFALSLT
                     LQLDLGAMADEERAEFCREMNVQPADRDHLIRRIMHASGQMIFFTAGEKEVRTWLLRQ
                     GGTAVEAAAGVHTDLAKGFIRAETMTVADLLRLGSEREIKAAGLMRQEPKDYVVRDGD
                     IITIKHN"
     gene            complement(1241..3655)
                     /locus_tag="VFB80_17180"
     CDS             complement(1241..3655)
                     /locus_tag="VFB80_17180"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZN35567.1"
                     /translation="MTVDRALQLNTTALAVTGAVFLGLGHESIVMPLLLAAGAIAAFT
                     LTDVLKWIRLNRLAANLVALVAVAWSLKDFFDTGRGSEQQLLAIANMLVYLQVVLLFQ
                     DKTPRLYWQLLVLSLLQAVVAAALGLGPLFGLLLAPYVLLALAHLLLLCLHHELAAPK
                     TLARPGAAAEPWRALLAPPVVSAGGNPAEELESAFGGRRIARQVVALGAATLLFTAVF
                     FYATPRLSDAAWQSGRGRGAVSGLANEVRLKESGSIQLSDQVVLRALLTRLNDRQPVV
                     VVGEPYFTGSTLTEYRVEDGVSRWVMGPRPSRAPMATSNAARRPLTNVPGPGEFPRPT
                     LLVRQDIVLEVGSTPTYPAIVPAQRLNDTPQHLTYRRMLNRVERIAPEGETQGREYRY
                     SLATPAIRGGRQLYAMPHPNPAATPTDPELSTLAQFEAGRFPQLAATAARILSEQNPP
                     AESSLQRALALQQHFHTPGLYRYSLELAERKRDLDPIEDFVANHRTGYCEYFASALVL
                     MLRSQGIPARMIIGYKGGELNSLGHYYVVKQRHAHAWVEAWLPAGDVPEWEIAGPVAG
                     GVWYRLDPTPGADEAAAVVTEESLARRVMHGFDYVDLLWREYVLSLNKTQQERSVYEP
                     LTARATALPAWLEARYVHRLLRQWSATLGIPVPPPTARGPRPFEGSLALLIAGGLLLA
                     FVLYQAAVFARWLFVRWWKALVAGRKARGHAPGFYLRLERLLARLPLRRRAGQTAREL
                     AAEAERRLQGAVSEEPSVAGLPEEIVAVYYRVRFGAARLDKTQAAAIEQALDRLAPAV
                     RQARPR"
     gene            3675..4325
                     /locus_tag="VFB80_17185"
     CDS             3675..4325
                     /locus_tag="VFB80_17185"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZN35568.1"
                     /translation="MRELLRPMLPMIVVLAIPIVPFVLFGQERVDAQLRKWREEPPPA
                     AFVSAAVIGLLASDIFLPIPSSAVSTLAGWQLGAVGGALVAWTGMSLGAAIGFALAKR
                     FGGPAVAWLTRAEDLDRTRRLAERFGPALLILGRGVPVLAEATVLLVGMHGLSWRRFL
                     PPVLLANLGLAIAYAAFGEIAGRHQWLPLALGVSIALPVLMVAAFRLAAKDTTIAP"
     gene            4322..6322
                     /locus_tag="VFB80_17190"
     CDS             4322..6322
                     /locus_tag="VFB80_17190"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HZN35569.1"
                     /translation="MTDSHASLAALLGADNPRLYDIQTAGHGPAGELPLTDEMLRTWS
                     SGDLFGLTQAAGMGWKPQDLLGPQYLILSTQGGVRAPDGTPIALGYHTGHWEVGLLVQ
                     AAAREVKALGGVPFAAYCSDPCDGRTQGTTGMFDSLPYRNDAAVVLGRLIRSLPQRRG
                     LIGVATCDKGLPAMMLALAAARDLPGAIVPGGVTLPPIDSEDAGKVQTIGARYVHGEI
                     SLHDAAVAGCAACGSPGGGCQFLGTAATSQVVAEALGMALPHSALAPSGQPIWLELAA
                     ASARAVASLSERGLAMCDIVTSASVRNAMVVHAACGGSTNLLLHVPAIAFAAGLPRPT
                     VEEWSDINRRVPRFVSVLPNGPVNHPTVRVFLAGGVPEIMLHLRESGLLDLGVLTVTG
                     QTLGEVLDWWQASQRRKAVRELLKARDGVEPDDVIIPPAEARRRGLTSTICFPRGNLC
                     PEGAVIKATAIDPAVVDADGVYRKTGPARVFTRERDAITAIKGQGTQPLRPGDVLVLI
                     GRGPMGSGMEETYQITSALKFLKWGRDVAVVTDARFSGVSTGACIGHVGPEALSGGPI
                     GKVRDGDLIQIIIDRNKLAGSVDLVGESLPSGPRRFSPAEGEVVLAARQPRADLAPDK
                     DLPADTRLWAALQRVGGGTWGGCVYDVEKIVAALEAGQRSAG"
     gene            complement(6329..7189)
                     /locus_tag="VFB80_17195"
     CDS             complement(6329..7189)
                     /locus_tag="VFB80_17195"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HZN35570.1"
                     /translation="MSIRVDCEKCGFEYKLRDELAGKKVKCKICQAVFVVPRPATGPQ
                     PVDTTGHGDPVYRYTERPTQFELAVGDSENIEQISKHIERHCGPIDGVMHELVSDLVH
                     IDIHSVKPTSERPYHTLVTSGMSDRAMITPEGAAEEVAKCQFAELMLCLPPQWPLDEE
                     SWKNPRHYWPIYLLKMLARFPHEYQTWLWWGHTMPNGDPAEPYADNTKLCCALLLSPL
                     RFAPEFHQLEVGPEKTIHFMSIVPLYQEEIDFKLNEGAEALCERFDKHGITEVLDVKR
                     VNVCQKRGWW"
     gene            complement(7272..8300)
                     /locus_tag="VFB80_17200"
     CDS             complement(7272..8300)
                     /locus_tag="VFB80_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35571.1"
                     /translation="MPRPSRAKPTLALESLESRQALAGDVMAVMYGQMLVIWGDAAAN
                     GVVLSYSSNLQTYEVAGQDAGGAPTTINSQAASASFSNVQSVAVLLNGGDDAFSVGSP
                     AAVDTAIRQWLSIDMGDGNDTVQLGRGGNAPGGNAPIAMHLRTGTSVNIDLGAGDDHL
                     ELANAEIGLALNVAAGDGNDDVIFATEFTPEGSSDAQLFPVVIRGNTGISLGGGEDEL
                     EILNSVLQGHVRILDGAGAAHINLDNVSIAKNLDIDTATSDDQLKINFVNARNVTIDS
                     NGGIDDVDFTNCRFRSMILKLGAQRDALLLRNVRVSFMTRLDGGDNGSRLAGSNNTLR
                     GLTRRNIG"
     gene            8505..9764
                     /locus_tag="VFB80_17205"
     CDS             8505..9764
                     /locus_tag="VFB80_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN35572.1"
                     /translation="MYLRLAKRLLLETDKRLLWKLAWNFGAKGVRSVQKHKARLKRGE
                     FFPPFLYISLINSCNLRCQGCWVDVAAKQARIEPAAMNRLITEAKAMGNSFFGILGGE
                     PFMHPEILEILGGHRDCYFQVFTNGHFITDDVARRLREFGNVTPLVSVEGNEIVSDER
                     RGREGVLSQTMAGLQNCLKHKVMTGVCTSLCQSNFDLLSEKWLDRLIEMGVMYTWFHI
                     YRPIGPDAKPELALTPEQQLECRKFVVEMRARKPIIIIDAYYDGEGRALCPAATGFTH
                     HISPWGDIEPCPIIQFAKESIYDERPLREVFQQSAFLRDFRETAARHTRGCIVLERPD
                     LLEDLARRHGAKDTTARHAAYSEVAALEKRPSQYNPGQEIPEKSLAYRLAKYFFFNDY
                     GTYTQHFDAARWIDTRGQEPPKLVQIG"
     gene            9856..11139
                     /locus_tag="VFB80_17210"
     CDS             9856..11139
                     /locus_tag="VFB80_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN35573.1"
                     /translation="MNAIFLRGFVAACAAIMLAAVARAADSTAPLRAEIRVGIAEGDI
                     RGSDGRAIQAAIDYVASLGGGTVRIGKGRYTIRNAVTLRDNVTLVGVTGETVLAACDG
                     VASKLTLDGDANERQITVADPGAFRVGDGVSIADKDNGGGFSVTTATLTAQTGPATFR
                     LSGPLYLDYLVSLGATAKLAFPLIGGWQVKDAAVEGLVLEGNRDKAQYLNGCRGGGIY
                     LFECERVAIRNCTVRGYNGDGISFQVSAHVTVEDCLVEKNAGLGIHPGSGSKSPVVRR
                     CRSLTNGSDGLYVCWRVTHGLFEDNEIRGNERAGVSIGHKDTDNLFRRNTIVQNLAAG
                     VLFRNESEAMGAHRNVFENNRILDNAADSKRNPAAAVVVQGPHHDVVFKDNTIGFTQA
                     GERPTIGILLSAEARNLQADQAQFQNVKTPIEIKK"
     gene            11253..12191
                     /locus_tag="VFB80_17215"
     CDS             11253..12191
                     /locus_tag="VFB80_17215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN35574.1"
                     /translation="MAANSGLSRRVFLQAGAALGGWSLGSQVFGQEQSAKLGKPTKFQ
                     IACMTLPYSQYPLARALSGIKSAGYEYVAWGTSHREEGESRPVPVMPADAPPAKAKEL
                     GQRCRDQGLSPLMMFSGIYPEDPKGVEVLTQRLKQADAAGIPHVLTFGHTSGNNRQVW
                     VERFKKLGPVARDLGVLLVVKQHGETGQVTGEIVREVADDNIKVNYDAGNVMDYTKGQ
                     VDPLKDIKTCAELIHSFCIKDHRMFPVNQDCGPGLGEIDHYRLLDNVAFTGREMPLCC
                     ENISAPKIRPTKPEEIDALAKRAREFLEIVIRGLHA"
BASE COUNT         2121 a         4085 c         4127 g         2140 t
ORIGIN      
        1 aacgccgccg aaagcctcct tgactttgcc ggtcgccacg tgccgcagga tgaatgccca
       61 gccgcccggc cccgggttgc cgctgcaagc gccgtcggtg aagaggagga cttcggggtc
      121 gaatgaatcc ggcaagagaa aacctgctga ggcgtcttca tgcggaatgc ggaatagttc
      181 gtattgccct aattccgcat tccgcattcc gtgcgcattt agttgtgctt gatcgtaata
      241 atgtcgccgt cgcggactac ataatccttc ggctcctgcc gcatcaggcc ggcggctttg
      301 atttcgcgct cgctgcccag gcggagcagg tcggcaactg tcatcgtttc ggcgcggatg
      361 aagcccttcg ccaggtcggt gtggacaccg gcggcggctt cgacggcggt gcccccttgc
      421 cgcagcagcc acgtccggac ttccttctcg ccggccgtga agaagatcat ctggcccgaa
      481 gcgtgcatga tccggcgaat aaggtggtcg cgatccgcgg gctgcacgtt catctcacgg
      541 cagaactcgg cccgttcttc gtccgccatg gcccccaggt caagttgcag cgtgagcgaa
      601 agggcgaaca gctcgctccc agcgggcgca agactcgcga aacgctgcgg ctgatcctcg
      661 tcgtcggccg tgttgacgat gatcagccgc ggcttctcgc tgaagagctg aaacgatctt
      721 gtagcccggc gttgctcggg gctcatggcc agcgcgtgca gcggtttgcc cgcttccaat
      781 tcggcgagga gcggcgtcag caggtccagt tcctcctggt ccttctcttt gttggcccgc
      841 ggcttgcgca tctggtcttt gaggcgctcg acgcgcccgg aaaggatgtc gaggtcggcg
      901 atcagcaggt cgtcgtcgaa gtttcgcagg tccgcggccg cgttgcctcc gccaaaggcc
      961 gcaacgacga tgacaagtgc gccggcctcg cggatcatcg ccagtttttg cgcggaacct
     1021 tcgtgcgtcc gcgccagacc cggcgtatcg acgatttcga gggccgcctg cgtgaccttt
     1081 ttcggctggt agattttgca gagttgcgcc acgcgcggat cgggaattgt cgccatcgcg
     1141 ctctgaatcg tgtgggcgag cgatggatcg ggcgagatcc cggtgagcca atgaaagagc
     1201 gtgctcttgc ccgagccctg gtaaccaaca attcctattt tcatcgcggc ctggcttgac
     1261 gaacggcggg agcgagacga tccagcgctt gttctatcgc ggctgcttgc gtcttgtcca
     1321 ggcgggcagc gccgaagcgc acgcggtaat agacggcgac gatctcctcc ggcagtcccg
     1381 ccacggacgg ctcttcgctg accgccccct gcagccgccg ctcggcttca gccgccaact
     1441 cgcgggctgt ttgccccgcg cggcggcgga gcggcaagcg ggccagcagc cgctccaggc
     1501 ggagatagaa tccgggcgca tggccgcggg ccttgcggcc ggcaaccagc gccttccacc
     1561 agcggacgaa gagccagcgg gcaaatacgg ccgcctgata taggacgaac gccaagagca
     1621 gcccgccagc gatcagcaac gccaaactgc cctcgaacgg ccgcgggccg cgggccgtcg
     1681 gcggcggaac agggattcct aaggtcgccg accattgccg cagcaagcga tgcacgtagc
     1741 gggcctcgag ccaggctggc aaagcggtgg cccgcgcggt tagcggctca taaaccgacc
     1801 gctcctgctg cgtcttattg aggctcagga cgtattcgcg ccacagcaag tcgacgtagt
     1861 cgaagccatg catcactcgg cgggccagcg attcctccgt cacgaccgct gccgcttcgt
     1921 cggcgccggg cgtcgggtcg agacggtacc agacaccgcc cgcaaccggc ccggcgatct
     1981 cccactccgg cacgtcgccc gccggcagcc aggcctcgac ccaggcatga gcgtgccgct
     2041 gcttcacgac atagtagtgg cccagcgaat tcagctcgcc ccctttgtag ccgatgatca
     2101 tccgcgcggg gatgccctgg ctgcgaagca tgagcaccag ggcgctggca aagtactcgc
     2161 agtagccggt gcggtggttg gcgacaaaat cctcgatcgg atcgagatcg cgcttccgct
     2221 ccgccagctc gagcgaatag cggtacagcc ccggcgtgtg aaaatgctgc tgcaaggcca
     2281 gggcccgctg cagcgacgat tcggccggcg gattctgctc gctaaggatg cgggcggccg
     2341 tcgcggccag ttgtggaaag cgcccggcct caaactgggc gagggtggac agttcgggat
     2401 cggtcggcgt agcggccggg ttaggatgcg gcattgcgta caactgccgg ccgccgcgga
     2461 tcgccggagt ggcgagcgaa tagcgatatt cgcgcccttg cgtttcgcct tcgggcgcaa
     2521 tccgctcgac tctgttgagc atccggcgat aggtcaagtg ctgcggcgta tcgttgagcc
     2581 gctgcgccgg cacgatggcc ggataggtgg gcgtgctgcc gacctcgagc acgatgtctt
     2641 gccgcacgag cagcgtcggc cggggaaact cgcccggacc aggcacgttg gtgagcgggc
     2701 ggcgggccgc attgctggtc gccatgggag cccgcgacgg gcgcgggccc atcacccagc
     2761 ggctgacgcc gtcctccacc cggtattcgg tcaaggtcga gcccgtgaaa tacggttcac
     2821 cgacgacaac gacgggctgg cgatcgttga gccgcgtgag cagggcccgc agcacgacct
     2881 gatcgctgag ctggatgctg cccgattcct tcaacctgac ctcgttggcc agtcccgaga
     2941 ccgcgccgcg gccgcgcccg ctctgccatg ctgcatcgct gagccgtggc gtggcgtaga
     3001 agaacacggc cgtgaacaac agcgtcgccg cccctaacgc gacgacctgc cgcgcgattc
     3061 gccggccgcc aaaagccgac tcaagctcct cggctgggtt gccgcccgca ctgacaacgg
     3121 gcggcgcgag cagcgctctc cagggctccg ccgcggcccc gggccgcgcc agcgtcttag
     3181 gagcagcgag ctcgtgatgc aggcacaaca gcagcagatg cgccagcgca agcagcacgt
     3241 aaggtgcgag gagcaagccg aacagcggtc ccaggcccag cgctgcggcg accaccgctt
     3301 gcagcaggct cagcaccagc agctgccaat aaagccgcgg agtcttatcc tggaagagca
     3361 gcacgacctg caagtagacc agcatgttgg cgatcgccag cagttgctgt tcgctgccgc
     3421 ggccggtatc gaagaagtcc ttgagcgacc aggccacggc cacgagggcg accaggttgg
     3481 cggcgagacg gttgagccgg atccacttca gaacgtcggt caaagtgaag gctgcaatcg
     3541 cgccggccgc cagcagcagc ggcatcacga tactttcgtg cccgagccca aggaacacag
     3601 cccccgtcac cgccagcgcg gtcgtgttaa gctggagtgc gcggtccacc gtcatttggt
     3661 gagattatag tggaatgcgg gaactgctgc gcccgatgct gccgatgatc gtggtcctgg
     3721 cgatcccgat tgtgcccttt gtcttattcg ggcaagagcg cgtcgatgcg cagctccgca
     3781 agtggcgcga ggagccgccg ccggcagctt tcgtttccgc ggccgtcatc gggctcttgg
     3841 cgagcgatat tttcttgccg attcccagta gcgcggtaag cacgctggcc ggctggcaat
     3901 tgggggcggt cggcggagcg ctcgtggctt ggaccggcat gagcctcggc gcggcgatcg
     3961 gctttgccct ggccaaacgc ttcggcgggc cggcggtcgc ctggctgacg cgggcggagg
     4021 atctcgaccg cacgcgccgc cttgccgagc ggttcgggcc agcactattg attctggggc
     4081 gcggcgtacc cgtgctcgcg gaggcgaccg tgctcctggt cggcatgcac ggcctgtcgt
     4141 ggcggcggtt cctgccgccc gtgctgttgg cgaatctcgg cctggcgatc gcgtatgccg
     4201 ccttcggcga aatcgccggc cgccaccagt ggctgccgct ggcgctaggc gtctcgatcg
     4261 ccttgccggt gctgatggtg gcggcgtttc gactcgcggc caaagacact acgattgctc
     4321 catgactgat tctcacgctt cgctcgcggc gctgctcggc gcggacaacc cgcggctgta
     4381 cgacattcaa accgctggac acggcccggc gggcgagctg ccgctgaccg acgagatgct
     4441 ccgcacctgg tcgagcggcg acctgttcgg gttgacgcag gcggctggca tgggctggaa
     4501 gccgcaggat ctgctcgggc cacagtatct gatcttgagc acgcagggcg gagtccgcgc
     4561 gccggacgga acgccgatcg cgctgggcta tcacacgggg cactgggaag tcgggctgct
     4621 ggtgcaggca gcggcccgcg aggtgaaggc tctgggaggc gtgccgtttg ccgcttactg
     4681 ttcggaccct tgcgacggcc gaacacaggg cacgacggga atgttcgata gcctgcccta
     4741 tcgcaatgat gcggccgtcg tgctcggccg cttgatccgc tcgctgccgc agcggcgagg
     4801 cttgattggc gtggccacgt gcgacaaggg cctgccggcg atgatgctgg ctctggcagc
     4861 cgcccgcgat ctgcccggcg caatcgtgcc gggtggcgtg acgctgccgc ccatcgatag
     4921 cgaagacgcc ggcaaggtgc agacgatcgg cgcgcgctac gtccacggcg aaatctcctt
     4981 gcacgacgcg gccgtcgcgg gctgcgcggc ctgcggctcg cccggcggcg gctgccagtt
     5041 cttgggcacg gccgcgacct cgcaagtcgt cgccgaagcg ctgggcatgg cgctgccgca
     5101 ttcagcgctg gcgccgagcg ggcagccgat ctggctggaa ttggccgcag ctagcgcgcg
     5161 ggccgtcgcg agcctgtccg agcgcggtct ggcgatgtgc gacatcgtca cttcggcaag
     5221 cgtcaggaac gccatggtcg tgcacgcggc ttgcggcgga tcgacaaacc tgctgttgca
     5281 tgtgccggcg attgcctttg ccgcggggct gccgcgcccc acggtcgagg agtggagcga
     5341 catcaatcgc cgcgtgccgc gctttgtctc ggtcttgccc aatgggccgg tcaatcatcc
     5401 gaccgtgcgg gtgttcctcg cgggcggcgt gccggagatc atgctgcacc tgcgcgaatc
     5461 ggggctgctc gatctgggcg tgctgactgt gacgggccag acgctcggcg aggtcctgga
     5521 ttggtggcag gccagccagc ggcgcaaggc ggtgcgcgaa ctgctgaagg cccgcgatgg
     5581 cgttgaaccg gacgacgtga ttatcccgcc ggccgaggcc cgccgccgcg gactgaccag
     5641 cacgatctgc ttcccccgcg gcaatctctg ccccgagggg gcggtgatca aggccacggc
     5701 gatcgatccg gccgtggtcg atgccgacgg cgtctatcga aaaaccggcc cggcccgcgt
     5761 cttcacccgc gagcgcgacg cgatcaccgc catcaaaggg caaggcacgc agccgctgag
     5821 gcccggcgat gtgctggtgc tgatcggccg cgggccgatg gggagcggca tggaggagac
     5881 ctaccagatt acgtccgccc taaaattcct caaatggggc cgcgacgtag ccgtcgtcac
     5941 cgacgcccgc ttctccggcg tcagcaccgg cgcgtgcatc ggccacgtcg ggcccgaggc
     6001 cctctccggc ggtccgatcg gcaaggtccg agacggcgat ctgatccaga ttatcatcga
     6061 ccgcaacaag ctggccggca gcgtcgatct ggttggtgag tcgctgccat ctggcccccg
     6121 ccggttctcg cccgccgagg gtgaggttgt gcttgccgcc cgccagccgc gggccgatct
     6181 cgccccggac aaggaccttc ccgccgacac ccgcctgtgg gctgccctcc agcgcgtcgg
     6241 cggcggcacc tggggcggct gcgtgtacga cgtggaaaag atcgtcgcgg cgctcgaagc
     6301 cggccagcgc tcggccggtt gagtgccgct accaccagcc gcgtttctgg cagacgttga
     6361 cccgctttac atccagcacc tcggtaatgc cgtgcttgtc aaaacgctcg cagagcgctt
     6421 cggcgccctc gtttagcttg aagtcgatct cttcctggta cagcggcacg atcgacatga
     6481 aatggatggt cttctccggg ccgacttcca attggtggaa ctccggggca aaacgcaacg
     6541 gcgagagcaa cagcgcgcag cacagcttgg tattgtcggc atacggctcc gctggatctc
     6601 cgttgggcat cgtgtggccc caccagagcc aggtctggta ctcgtgcgga aagcgggcca
     6661 gcatcttgag caggtagatg ggccagtagt gccgcggatt cttccaggat tcttcgtcga
     6721 gcggccactg cggcggcagg cagagcatca gctcggcgaa ctggcacttt gcgacttcct
     6781 cggccgctcc ttccggcgtg atcatcgccc ggtcgctcat gccgctggta accagcgtgt
     6841 gatacggccg ctcgctggtt ggcttgaccg aatggatgtc gatgtgcacc aggtccgaga
     6901 ccagctcgtg catcactccg tcgatcggcc cgcaatgccg ctcgatgtgc ttggaaatct
     6961 gctcgatatt ctccgaatcg ccaaccgcca actcgaactg cgtcggccgc tcggtgtagc
     7021 gatagaccgg gtcgccgtgg cccgtggtat cgaccggctg cggccccgtg gccggccgcg
     7081 gcacgacaaa gaccgcctgg cagatcttgc acttgacctt cttgccggcc agctcgtcgc
     7141 ggagcttgta ctcgaagccg cacttctcgc aatcgactcg aatcgacatc gtggcgctcc
     7201 gtggagagtc acgcgggcac tcgaccacga cactataggg ccgcgggcaa cgccattcca
     7261 cttgcgcggt cctagccgat gttgcgccgt gtcagcccgc ggagcgtatt gttcgagccg
     7321 gccagccggc tgccgttgtc gccgccgtcg agccgcgtca taaacgaaac gcggacattg
     7381 cgcagcagca gggcgtcgcg ctgggcgccc agcttgagga tcatcgagcg gaagcggcag
     7441 ttggtgaaat cgacatcgtc gatgccgcca ttgctgtcga tcgtcacgtt gcgggcgttg
     7501 acgaagttga tctttagctg gtcgtcgctg gtggccgtgt cgatgtcgag gtttttggcg
     7561 atgctgacgt tgtcgaggtt gatgtgcgcc gcgcctgcgc cgtcgagaat tcgcacgtgc
     7621 ccctgaagga ccgaattcag aatctccaac tcgtcctcgc cgcctcccag gctgatcccg
     7681 gtgttgccgc gtatgacgac gggaaacagc tgcgcgtcgc tggagccctc gggggtaaac
     7741 tctgtcgcga agatcacgtc gtcgttgccg tcgccggcgg cgacgttcaa tgccaggccg
     7801 atctcggcgt tcgccagctc caggtggtcg tcgcccgcgc ccaggtcgat gttgacgctc
     7861 gtgcccgtgc gaagatgcat ggcgatcggt gcattcccgc ccggcgcgtt gccgccccgg
     7921 ccgagctgaa ccgtgtcgtt gccgtccccc atgtcgatcg aaagccactg gcgaatcgcc
     7981 gtatcgaccg ccgcgggcga gccaaccgaa aaggcgtcgt ctccgccgtt gagcaggacg
     8041 gccaccgatt gcacgttgct aaacgaagca gacgcggcct ggctgttgat cgtcgtcggc
     8101 gccccgcccg cgtcttggcc cgcgacttcg taggtctgca agttcgagct gtagctgagc
     8161 accacgccgt tggccgccgc gtcgccccag atcacgagca tctgcccgta catgaccgcc
     8221 atcacgtcgc cagcgagggc ctgcctggat tccagcgatt cgagcgcgag cgtcggcttg
     8281 gcgcgagagg ggcggggcat gcgcttcctg ttccgtccga gagtggaccg tccgtgactg
     8341 cccccggctg acgtacgcgg ctgcggctgg gccggttcgc ggcggcggat gctaccggtt
     8401 tgtgcgcggc cgaccaagag caatggcatg gccatgactg catgactgca tgctggtacg
     8461 agctataatg ctcctttgcc cgtcagccac cagccacgca cccgatgtac ctccgcctcg
     8521 ccaaacgcct gctgctggaa accgacaagc gcctgctctg gaagctggcc tggaattttg
     8581 gggccaaggg cgtccgctcg gtgcagaagc acaaggcccg tttgaagcgg ggcgagttct
     8641 ttccgccgtt cctctatatc tcgctcatca acagctgcaa cctccgctgc caaggctgct
     8701 gggtcgatgt ggcggccaag caggcgcgga tcgagccggc ggcgatgaac cgcctgatta
     8761 ccgaagcgaa agcaatgggc aattcgttct tcggcatcct gggcggcgag cccttcatgc
     8821 acccggagat cctggaaatc ctcggcggtc atcgcgactg ctatttccag gtcttcacca
     8881 acggccactt catcacggac gacgtcgccc gccggctccg cgaattcgga aacgtgaccc
     8941 cgctggtcag cgttgagggg aacgagatcg tcagcgacga gcgccgcggc cgcgagggcg
     9001 tgctcagcca gaccatggcc gggttgcaga actgcctgaa gcacaaggtg atgaccggcg
     9061 tctgcaccag cctctgccaa tcgaacttcg acctgctcag cgagaagtgg ctcgaccggc
     9121 tgattgagat gggcgtgatg tacacgtggt ttcacatcta ccggcccatc ggccccgatg
     9181 cgaaaccgga attggcgctg acgccggagc agcagctgga gtgccgcaag tttgtcgtcg
     9241 agatgcgggc aaggaagccg atcatcatca tcgacgccta ctacgacggc gaaggccgcg
     9301 cgctctgtcc cgcggcgacc ggcttcacgc accacatcag cccttggggc gacatcgagc
     9361 cctgcccgat catccagttc gccaaagagt cgatttacga cgagcggccg ctgcgagaag
     9421 tctttcagca gtcggccttc ctccgcgact ttcgcgaaac ggccgcccgg cacacccgcg
     9481 gctgcatcgt tctggagcga ccggatctgc tcgaggatct cgcccggcgg cacggtgcca
     9541 aggacacgac ggcccgccac gccgcctatt cagaagtcgc cgccctcgaa aagcgaccca
     9601 gccagtacaa cccgggccag gaaatccccg agaagtcgct cgcctatcgc ctcgccaagt
     9661 acttcttctt caacgactac ggcacctaca cgcaacactt tgacgccgcc cgctggattg
     9721 atacccgcgg ccaggagccg ccgaagcttg tgcagattgg atagctggga acgtcatggt
     9781 ccagttgcct gggaggatga catgcggcgt ctcccgccag cggatcgccg acccagaacc
     9841 caccggagag aagccatgaa cgcgattttt ctgcgcggct ttgtcgccgc gtgcgctgcg
     9901 attatgcttg ccgcggttgc tcgtgccgcc gattcgaccg cgccgctgcg ggccgagatt
     9961 cgcgttggaa tcgcggaggg agatatccgc ggcagcgacg gccgggccat tcaggcggcg
    10021 atcgactacg tggcgagcct gggcggcggc acggtccgca tcggaaaggg gcgatacaca
    10081 atccgcaatg cggtgacgct gcgcgacaac gtcacgctgg tcggcgtcac cggcgagacc
    10141 gttctggccg cttgtgatgg agtcgcgtcg aagctgactc tcgacggcga cgccaacgag
    10201 cggcagatca ccgtcgccga cccgggcgca ttccgcgtcg gcgacggcgt gtcgattgcc
    10261 gacaaggaca acggcggcgg cttctcggtc accacggcga cgctcaccgc ccagaccggt
    10321 cctgcgacgt ttcgcctgtc cggcccgctc tatcttgact acctcgtttc actgggcgcc
    10381 accgcgaagc tggcgtttcc gctcatcggc ggctggcagg tcaaggacgc ggccgtggaa
    10441 ggtctcgtgc tcgaaggcaa ccgcgacaag gctcaatacc tcaacggctg ccgcggcggc
    10501 ggcatttacc tgttcgagtg cgagcgcgtg gcgatccgca actgcacggt ccgcggctac
    10561 aacggggacg ggatcagctt ccaggtctcg gcccacgtca ccgtcgagga ttgcctcgtg
    10621 gagaagaacg ccggactggg cattcatccg gggagcggtt cgaagtcgcc ggtggtgcgg
    10681 cggtgccgct cgctgaccaa cggcagcgac ggcctgtatg tctgctggcg cgtgacgcac
    10741 ggcctgttcg aagacaacga aatccgcggc aacgagcggg ccggcgtctc gattggccac
    10801 aaggacaccg acaacctctt ccgccgcaac acgatcgtgc agaacctggc ggcgggggtg
    10861 ctcttccgca acgaatcgga ggccatgggc gcgcaccgca acgtctttga gaacaaccgg
    10921 atactcgaca acgccgccga ttccaagcgc aatcccgccg ccgcggtggt cgtgcaaggc
    10981 ccgcaccacg acgtcgtctt caaggacaac acgatcggct tcacgcaagc cggcgagcgg
    11041 ccgacgatcg gcatcctcct gagtgccgag gcccgcaacc tgcaagccga tcaggcccag
    11101 tttcagaacg tcaagacgcc gatcgaaatc aaaaagtgat cactggccgc attcgccaaa
    11161 gaggagtgcg agattctgga caggcgcgtt tggggcggcg acaattcgag ccacaagtcg
    11221 ttgatcgcga gtcgcttgca ttgaggtgaa gtatggcggc gaattcgggg ctgtcgcggc
    11281 gcgtgttcct gcaagccggt gcagcactgg gcggctggtc gctgggcagt caggtctttg
    11341 ggcaggagca gtcagcgaag ctcggcaagc cgacgaaatt tcagatcgcc tgcatgacgc
    11401 taccctacag tcagtatccg ctggcgcggg ctttgagtgg gatcaagtcg gcgggctacg
    11461 agtatgtggc ctggggcacg tcgcaccgcg aagagggcga atcgcggccc gtgcccgtga
    11521 tgccagccga cgcgccgccg gccaaggcca aggaactggg ccagcggtgt cgcgaccagg
    11581 gcctgtcgcc gctgatgatg ttctcgggca tctatcccga agaccccaag ggcgtggaag
    11641 tgctcacgca gcgcctcaag caggccgacg ccgcgggcat tccgcacgtg ctgaccttcg
    11701 gccacaccag cggcaacaac cggcaggtct gggtcgagcg gttcaagaag ctcggaccgg
    11761 tcgcccgcga tctgggcgtc ttgctggtcg tcaagcagca cggcgagacg gggcaagtca
    11821 ccggcgagat cgtccgcgaa gtggccgacg acaacatcaa ggtcaattac gacgccggca
    11881 acgtgatgga ctacactaag gggcaggtcg atccactcaa agatatcaag acctgcgccg
    11941 agctgatcca cagcttctgc atcaaggacc accggatgtt cccggtgaat caggactgcg
    12001 ggcccggcct gggcgagatc gaccactacc gcctgctgga caacgtcgcc ttcaccggcc
    12061 gcgagatgcc gctgtgctgc gagaatatct cggcccccaa aatccgcccg acgaagccgg
    12121 aagagatcga cgccctggcc aaacgagccc gcgaattcct ggaaatagtg atccgggggc
    12181 tgcacgcgta gccgtcttca aagccgcgac cactggtcgc gctgcactgg tagagcgcga
    12241 ccactggtcg cggctttggg ggtgggcact ctttcctcgc agctgctccg cgctacgatt
    12301 aggatgaaat gcgcggcctg gggcccgccg cgcggcctca cgcggagcgt gaggaataca
    12361 atgatgaagc tgacgcccgt tcaagagctg gaagccgcga ttcgcgatct gccggccaat
    12421 ccagagcctt atctggcgat cgtcccgctg tatctggagc acggccgcga tta
//
LOCUS       DASRJB010000649         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1877915, whole genome shotgun sequence.
ACCESSION   DASRJB010000649 DASRJB010000000
VERSION     DASRJB010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877915"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFB80_17220"
     CDS             complement(<1..961)
                     /locus_tag="VFB80_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35575.1"
                     /translation="MRKPKSFLRVFTDTGPLVALAVAIALLIGIVGPASAQFFNFGGP
                     SRPAPRSGGGWFGGDFFQPFQQQAPQAPRQDFSRAPGPAKRDTVPERNVLVLGDAMAD
                     WLGYGLEDAYAEQPDMGVIRKHKTISGLIKYQPRGEPADWAAAAKAILATEKPDAIVV
                     MLGLNDRVSIREAAPEKKSDKKDDKKDARAKPGDAKSKPESSTDKSDAGKSDTAKADG
                     TKADTAKTDTAKADDADDDDTPQIAAPEKRAGGGLYEFRDERWVELYAKKIEELIGVL
                     KSKGVPVLWVGLPAIRGQKGTADMLFLDALYRDGAGKAGITYVD"
     gene            complement(1120..>2150)
                     /locus_tag="VFB80_17225"
     CDS             complement(1120..>2150)
                     /locus_tag="VFB80_17225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZN35576.1"
                     /translation="SPDLRIMDLMDSQPEFTKAIWDYLDILVNDNRLAKGREILAKYK
                     PQFDAVEKAYGVDRYAVASIWGIESNYSTQMGDRNVVQSTATLACVGRRQAYFKDEFL
                     TALEILNRGDLRPEQMRGSWAGAFGPTQFMPTAFKRYAVDADGDGRRDVVDNAADLIA
                     STANNLKKDGWQTGRTWGYEVVLPEGFNFMLADKAKAMTIAQWQSQGMKRADGKPFPD
                     ATEKAYLLAPAGTQGPGFLMVQNFRVIMKYNPAEAYALAIGHFADRLRGGAPFVQPWP
                     RQERVLSRAERLELQQLLAQRGFYKGTPDGQFGGQTREALRSFQASIGAPADGFASSD
                     VLERLRGR"
BASE COUNT          338 a          714 c          694 g          404 t
ORIGIN      
        1 catcgacata ggtgatgccg gccttgccgg caccgtcgcg atacagcgca tccaggaaca
       61 gcatgtcggc cgttcccttt tgcccgcgga tcgcaggcag accgacccac aacaccggca
      121 cgcccttgga tttcagcacg ccaatcagct cttcgatctt cttggcgtag agttcgaccc
      181 agcgctcgtc gcggaattcg tagagcccgc cgcccgcgcg cttttcgggg gctgcgattt
      241 gcggcgtatc atcgtcgtcg gcgtcgtcag ccttcgcggt atcggtcttg gcagtgtctg
      301 ccttggttcc atcagccttg gcagtatcgg acttgccggc atcagactta tcagtcgagc
      361 tttcaggctt ggacttcgca tcgcccggct tggcccgcgc gtccttcttg tcgtccttct
      421 tgtcgctctt cttctccggc gcggcttcgc ggattgacac gcggtcgttg aggccgagca
      481 tgacgacgat ggcatccggt ttttcggtag cgaggatggc cttggcggcc gcggcccaat
      541 cggcgggctc gccgcgcggc tgatacttga tcaggccgga gatggtcttg tgcttgcgga
      601 tcacgcccat gtcgggctgc tcggcatagg cgtcttccag gccatagccg agccagtcgg
      661 ccatggcgtc gcccagcacc agcacgttac gttccggcac cgtgtcgcgc ttggcaggcc
      721 cgggcgcacg ggagaaatcc tggcgcggcg cctgcggggc ctgttgctgg aagggctgaa
      781 agaaatcgcc gccgaaccag ccgccgccgc tgcgcggggc cggccgcgac gggccgccga
      841 aattgaaaaa ctgcgccgac gcagggccaa cgatcccgat caaaagcgcg atcgcaaccg
      901 ccagcgccac cagcgggccg gtgtcggtaa acacgcgcaa gaaggacttt ggctttcgca
      961 tccggctcgg tcgtaatggg tcgtgatgtg tcaaatatag taacggaatc gggccgcaag
     1021 cgggcagatt ttccccataa accggggttc cggcctgacc gtcaaggctt ccgcccgaat
     1081 cgtggctttc agggttactc taggggcaaa aagcgggctt taccgccccc gcagccgctc
     1141 cagcacgtcg gacgaggcaa agccgtccgc cggcgccccg atcgaggcct ggaagctgcg
     1201 cagcgcctcc cgtgtctggc cgccaaactg gccgtccggc gtgcccttgt agaagccgcg
     1261 ctgggccagg agctgctgca gttccagccg ctcggcgcgc gacagcaccc gttcctgccg
     1321 cggccagggc tgcacgaacg gcgccccgcc gcgcaggcgg tcggcaaaat ggccgatcgc
     1381 cagcgcatag gcctccgccg ggttgtattt catgatcacg cggaaattct gcaccatcag
     1441 gaagccgggc ccctgcgtgc cggccggcgc cagcagatag gccttctcgg tcgcgtcagg
     1501 gaacggcttg ccgtcggccc gcttcattcc ctggctctgc cactgcgcga tcgtcatcgc
     1561 cttggctttg tccgccagca tgaaattaaa gccctccggc agcaccacct cgtagcccca
     1621 ggtccggccg gtctgccagc cgtccttctt gaggttgttg gcggtcgacg caatcaggtc
     1681 ggcggcgttg tcgacgacgt cgcggcggcc gtcgccgtcg gcgtccaccg cgtagcgctt
     1741 gaaggcggtc ggcatgaact gcgtcggccc gaacgcgccg gcccaggagc cgcgcatctg
     1801 ctcaggacgc agatcgccgc ggttgaggat ttccagcgcg gtgaggaatt cgtccttgaa
     1861 ataggcctgg cggcggccga cacaggccag cgtcgcggtc gattgcacca cgttgcggtc
     1921 gcccatctgc gtcgagtagt tcgactcgat gccccagatc gaggcgaccg cgtagcggtc
     1981 gacgccgtag gctttctcca ccgcgtcgaa ctgcggcttg tacttcgcga ggatctcgcg
     2041 gcccttggcg aggcggttgt cgttcacgag gatgtcgaga tagtcccaga tcgccttggt
     2101 gaattcgggc tgcgaatcca tcaggtccat gatgcgcagg tcgggcgaca
//
LOCUS       DASRJB010000650         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1878391, whole genome shotgun sequence.
ACCESSION   DASRJB010000650 DASRJB010000000
VERSION     DASRJB010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878391"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFB80_17230"
     CDS             <1..303
                     /locus_tag="VFB80_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZN35577.1"
                     /translation="IVILLKGQATAITDGSQTRINATGNPGLATGGTGDVLTGVITAL
                     LCQSLAPLDAAALGAHVHGLAGDLAAAELGQVSLIASDLLRYLPKAFLAASGERRA"
     gene            380..1333
                     /locus_tag="VFB80_17235"
     CDS             380..1333
                     /locus_tag="VFB80_17235"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZN35578.1"
                     /translation="MRLLRSLDNLPADLRRGAVTIGNFDGVHRGHARLVERLLAKARE
                     VGGPAIVFTFDPHPVRLLRPESAPPPLTWTDRKAQLLDELGVTAVIAYPTDEALLALS
                     AEAFFAKIVLEQLAARAMVEGPNFYFGRGRAGNVESLQQLCSRSSVALEIVPPIGVGD
                     QLVSSSRVRDAISAGDIDLARQLLTRPYRVRGMVTHGAGRGAKIGFPTANLEAIDTLL
                     PAQGVYAARAIAPAGVWPAAVNIGPNPTFGEHALKVEAHLIDFSGPLYGQPLELDFLS
                     RLRSVRPFESVAALKSQLAHDVAAAREFASRDATAEEDTVG"
     gene            1427..>2096
                     /locus_tag="VFB80_17240"
     CDS             1427..>2096
                     /locus_tag="VFB80_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZN35579.1"
                     /translation="MAINWPRFAEIVRSRQRFLLVSHIRPDCDALGSELGMAGVLEAL
                     GKDVRIVNGQATPPNLAFIDPARRIGVIGQTVQPAELADRELLMILDTSAWAQLGPMS
                     EFIRAFQGIKIVVDHHVSADDLGAEPFKNVEAEATGRLVVEAAEALSVKLTPQMAAPL
                     FAAVATDTGWFRFSSTTAVTYRVAAKLIEAGANPAAIYNQLYEQDTLGRMKLRGVILS
                     RIETE"
BASE COUNT          342 a          711 c          700 g          343 t
ORIGIN      
        1 atcgtgatcc tgctcaaagg gcaggcgacc gccatcaccg acggctcgca gacgcggatc
       61 aatgcgaccg gcaatccggg cctggcgacc ggcggcaccg gcgacgtgct cacgggcgtc
      121 atcacggcgc tgctctgcca gagccttgcg ccgctcgacg ccgcggcgct gggcgctcac
      181 gttcacggcc tggcgggcga tctggccgcc gccgagctgg gacaggtctc gctgatcgcc
      241 agcgatctgc taaggtactt gccaaaggcg ttcctcgcgg ccagcggcga gcggagggcg
      301 tgagccccct gaggtcgcgc agcacgtggg tcgcccgctg ggccagagtg ggctgacgcc
      361 caccgctcgc cggaaaacta tgcgtctcct ccgctcgctc gacaacctgc ctgccgacct
      421 gcgccgtggc gcggtgacga tcggcaattt tgacggcgtc catcgcgggc atgcgcggct
      481 ggtcgagcgc ttgctggcca aggcccgcga ggtcggcggg ccggcgatcg tgttcacgtt
      541 cgatccgcat ccggtgcggc tgttgcggcc cgaatcggcg ccgccgccct tgacctggac
      601 cgaccgcaag gcgcagctgc tcgacgagct gggcgtgacg gcggtcatcg cttatcccac
      661 cgatgaagcg ctgctggcac tctcggccga ggcgttcttt gcaaaaatcg tgctcgagca
      721 attggcggcc cgcgcgatgg tcgaggggcc gaacttctac tttggccgcg ggcgggcggg
      781 caacgtcgaa tcgctgcagc aactctgctc gcggtcgagc gtcgccctcg aaatcgtgcc
      841 acccatcggc gtcggcgacc agctggtttc cagcagccgg gtccgcgatg cgatctccgc
      901 cggcgacatc gatctggcgc ggcagctgct gacgcggccc tatcgcgttc gcgggatggt
      961 gacgcacggg gccggccgcg gcgcgaagat cggattcccc acggccaatt tggaagccat
     1021 cgacacgctg ctgccggccc agggcgtgta cgccgcgcgg gccatcgcgc ctgccggcgt
     1081 ttggcccgcg gccgtcaata tcggacccaa tccgacgttc ggcgagcacg ccctcaaggt
     1141 ggaagcgcac ctgattgact tcagcggccc gctctatggg cagccgctgg agctcgattt
     1201 tctctcgcgg ctgcggagcg tccggccgtt tgaatcggtc gcggcgctca agagccaact
     1261 cgcccacgac gtggcggccg cccgcgaatt cgccagccgc gacgccacgg ccgaagaaga
     1321 cacggtcggt tgacagctaa cagttcaagg ttcaaagttc aaagttcaag gttgagggct
     1381 tgctttcctg catcaacttt gaaccttgaa ccttgaactt tgaaccatgg caattaactg
     1441 gccacgcttc gccgaaatcg tccgcagcag gcagcgattc ttgctggtga gccacatccg
     1501 gcccgattgc gacgcgctgg ggagcgagct gggcatggca ggtgtgctgg aggctctggg
     1561 caaggacgtg cggatcgtca acggccaggc gacgccgccc aacctggcgt tcatcgatcc
     1621 cgccaggcgg atcggcgtca tcggccagac ggtgcagccg gcggaactcg ccgaccgcga
     1681 gctgctgatg atcctcgaca cgagcgcttg ggcgcagctc gggccgatga gcgaattcat
     1741 ccgcgccttc cagggcatca agatcgtcgt cgatcatcac gtcagcgccg acgacctggg
     1801 cgccgagccc ttcaagaacg tcgaggcgga ggcgaccggc cgactggtcg tggaggccgc
     1861 cgaggcgctc agcgtcaagc tcacgccgca aatggccgcg ccgctctttg ccgcagtcgc
     1921 caccgacacc ggctggttcc gcttttcctc gaccacggcc gtcacctatc gcgtcgcggc
     1981 gaagctgatc gaagccggcg cgaatccggc ggcgatctat aaccagctct acgagcagga
     2041 cacgctcggc cggatgaaac tgcgcggcgt gatcctgtcg cggatcgaga cagaac
//
LOCUS       DASRJB010000651         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3855446, whole genome shotgun sequence.
ACCESSION   DASRJB010000651 DASRJB010000000
VERSION     DASRJB010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3855446"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2835
                     /locus_tag="VFB80_17245"
     CDS             <1..2835
                     /locus_tag="VFB80_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN35580.1"
                     /translation="RDELAQIKGVGDVTYLGQLDYSMRVWLDPEKMAARDLSASDIAN
                     ALREQNVQVAAGALGRPPVPQGQAFQYTLSTLGRLTGPEEFGSIVVKSGDQGQLTRLR
                     DVVTDVRTDEHGRKFGGIQLGAKVEDTSCSLDGEPSIGLGIFQQPGSNALKTAEAIGT
                     RMEELKRGFPPDLDYAIVYDTTPFIEESIHEVFKALRDAIILVAIVVLAFLQSWRATL
                     IPLIAVPVAIVGTFAVMLGMGFSLNNLSLFGLVLAIGIVVDDAIVVVEAVEHHLEHGL
                     SPREAARKAMDEVTGPIIAISLVLMCVFIPCAFITGITGQFFRQFALTIAVSTFFSAV
                     NSLTLSPALCALLLRPKDQQRDPLTRVINLLLGWFFKLFNLGFKYAADGYSQSVRLLL
                     KVSVIALAVYAGLLYLTYFSFTRVPTGFIPSQDKGYLLVDVRLPDSASLERTQAVMAQ
                     IEKIARGDRPASAGLSSSGHSSGEHGGGGAHGTGGGVPGIAHTIAISGQSIVQNAIGS
                     NYGTVYVVLDEFHHRHGSELGADAIASKLRAECYREVQEAQVAVFGAPAVDGLGSAGG
                     FKVMVRDRAALGLDALQEAADGLAATGNEQKGLVGLFSAFRAQTPQMYVDIDRERCKA
                     MGVPLNEVFLTLQLYLGGFYSNDFNQFGRTWQVNLQSDPRFRLSPEQVRQLKVRNLKG
                     EMVPLGSVASVSPQGGPALVIRYNGVTAAAVNGSSLPGTSSGTVIRTMDDLAARELPQ
                     GMDTLWTELTLLQIRAGNTAIIVFAVAVVLVFLVLAAQYESLTLPLAVILVVPMCLLC
                     SVVGVALAGMDINIFVQIGFVVLVGLASKNAILIVEFAKEKSAHGMAPADATVEACRL
                     RLRPIIMTSLAFILGVVPLALAVGAGAEMRSTLGIAVFSGMLGVTLFGIFLTPVFYYV
                     IVSLFGQPAAAPAASGDPPGEDTAHPAKAA"
     gene            complement(2862..4040)
                     /locus_tag="VFB80_17250"
     CDS             complement(2862..4040)
                     /locus_tag="VFB80_17250"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZN35581.1"
                     /translation="MDDYALVLNAGSSSLKFTVFQRPASGGWPVEARGQIEGIGTAPR
                     LTVKDAAGESLARQEVAVQGAREAVAAVAAWLRSKYGGTRVLGVGHRVVHGGPRFAGP
                     TIVSPDVLAELRQLIPLAPLHQPYNLAAIEAIGERLPDVPQVACFDTSFHRGRPAVTE
                     LVPLPRELREAGLQRYGFHGLSYEYIASALPQFAPEIAGGRVIVAHLGSGASLCALRG
                     GKSIDHTLGFTALDGLCMGTRPGALDPGIVLHLFQSLQFSAAEVETLLYKKSGLLGIS
                     GISSDMRDLLARDEPEARLAVDYFVYRAAKEIGALAAVLGGIDAMVFTAGIGENSAEI
                     RRRICESSAWLGLALDAQANASGGPRITAPQSRVSAWVIPTNEELMIARHTARVLGWA
                     "
     gene            complement(4050..4982)
                     /locus_tag="VFB80_17255"
     CDS             complement(4050..4982)
                     /locus_tag="VFB80_17255"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HZN35582.1"
                     /translation="MKPETGTGKYEQLLARCKSLPPVPAAVVHPCDAAALSGAAQAWK
                     LGLLAPLLVGPRAKITAAAAAAGIDLETLPIIDVPHSHAAAAKAVELVREGQAEMLMK
                     GSLHTDELMAAVVARDGGLRTARRISHVFVMDVPTYHKVLIVTDGAINIAPALEDKAD
                     ICQNAIDLAISLGLAQPKVAILAAVETVTSKMPATVDAAALCKMAERGQITGGILDGP
                     LAFDNAISAEAARIKGIRSAVAGDPDILLAPDLEAGNILAKQLSFLANADSAGLVLGA
                     RVPIILTSRADSVRSRIASCGVAMLVAHARRKKP"
     gene            complement(4997..5509)
                     /locus_tag="VFB80_17260"
     CDS             complement(4997..5509)
                     /locus_tag="VFB80_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35583.1"
                     /translation="MSDEQPKRRSQADEDLEREIREGRKFSLAEAIGRLAGPGMMKGV
                     SPATLKQQADAEIESFLERHLVGPAGAISVVLLRQVRESELLLNNLEQPLVVLAGYIQ
                     RVLDSEYLLQELVRESDVEWGRVYGERPYFEKEGSPPHEEDPYTIDSVRRTLTQLLEK
                     LSAGARPTAG"
     gene            complement(5651..>5901)
                     /locus_tag="VFB80_17265"
     CDS             complement(5651..>5901)
                     /locus_tag="VFB80_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterobactin esterase"
                     /protein_id="HZN35584.1"
                     /translation="NSEKKPLRIFTHVSENDNRAKDPEETYHNWVMANERTAAALQAK
                     GYDYRYVFSKATRHCDSKVFEQTLADTLVWTWRGYRPE"
BASE COUNT          933 a         2030 c         1934 g         1004 t
ORIGIN      
        1 cgcgacgagc tggcccagat caagggcgtc ggtgacgtga cctacctcgg gcagctcgac
       61 tacagcatgc gggtctggct cgaccccgag aagatggccg cccgcgacct gtcggccagc
      121 gacatcgcta acgccctccg cgagcagaac gtgcaggtcg ccgccggcgc cctcggccgt
      181 ccgccggtgc cgcaggggca ggcctttcaa tacacgctca gcacgctcgg ccgcctcacc
      241 ggcccggagg agttcggctc gatcgtcgtg aagtccggag accagggcca gctgacgcgg
      301 ctgcgggatg tcgtgaccga cgtccgcacc gacgagcacg ggcggaagtt cggcggcatc
      361 cagctcggcg cgaaagtcga agacacgtcg tgcagcctcg acggcgagcc ctcgatcggc
      421 ctgggcatct tccagcagcc gggcagcaat gccctgaaga ccgccgaagc gatcggcaca
      481 cggatggaag agctgaagcg cggctttccg ccggacctgg actacgcgat cgtctacgac
      541 acgacgccgt tcatcgaaga gtcgattcac gaggtcttca aggccctccg cgacgccatc
      601 atcctggtgg cgatcgtggt gctggccttt ttgcagtcct ggcgggcgac gctgattccg
      661 ctgatcgccg tgcctgtggc gatcgtcggt accttcgccg tgatgctggg catgggcttc
      721 agcctcaaca acctctcgct gttcgggctg gtgctggcga tcggcatcgt ggtcgatgac
      781 gcgatcgtcg tcgtggaagc cgtcgagcat cacctggagc acggcctgtc gccccgcgag
      841 gccgcccgca aggcgatgga cgaagtgacg gggccgatca tcgccatctc gctggtgctg
      901 atgtgcgtgt tcatcccctg cgcgttcatc accggcatca ccggccagtt cttccgccag
      961 ttcgccctca cgatcgccgt ctcgaccttc ttctcggccg tcaactcgct gacgctcagc
     1021 ccggccctct gcgccctgtt gctacggccc aaggaccagc agcgcgatcc gctcacccgc
     1081 gtcatcaacc tgctcctcgg ctggttcttc aagctgttca acctcggctt caagtacgca
     1141 gccgacggct acagccagtc ggtgcggctg ctcctcaagg tcagcgtcat cgccctggcg
     1201 gtttacgccg gactgctcta cctgacctac ttcagcttca cgcgcgtgcc caccggcttc
     1261 atcccgtcgc aagacaaggg ctatctgctg gtggacgtgc ggctgcccga ctcggcgtcg
     1321 ctggagcgga cgcaagcggt gatggctcag atcgaaaaga tcgcccgcgg cgaccgcccg
     1381 gccagcgccg gactctcaag cagcgggcac agcagcggcg agcacggcgg cggaggtgcg
     1441 cacgggaccg gaggcggcgt ccccggcatc gcccatacga tcgcgatttc cggccagtcg
     1501 atcgtgcaga acgcgatcgg ctcgaactat ggcacggtct acgtggtgct ggacgaattt
     1561 catcaccggc acggcagcga gctgggggcc gatgcgatcg cgtcgaagct gcgggcggag
     1621 tgctatcgcg aggtccagga ggcccaagtg gcggtgttcg gcgcaccggc cgtcgatggg
     1681 ctcggcagcg cgggcggctt caaggtcatg gtccgcgacc gggcggccct gggactcgac
     1741 gccctccagg aagcggccga cggtctggcc gccaccggca acgagcagaa ggggctggtc
     1801 ggcctgttca gcgcgttccg ggcgcagacg ccccagatgt atgtcgacat cgaccgcgag
     1861 cggtgcaagg cgatgggcgt cccgctcaac gaggtgttcc tcacgctgca gctctacctg
     1921 ggcgggttct acagcaacga cttcaaccag ttcgggcgaa cctggcaggt caacctgcaa
     1981 agcgatccgc ggttccggct gagccccgag caggtgcggc aattgaaggt ccgcaacctc
     2041 aagggggaga tggtcccgct gggcagcgtc gccagcgtca gcccccaggg cgggccggcc
     2101 ctggtcattc gctacaacgg cgtcacggcc gcggcggtca acggcagctc gctgccgggc
     2161 accagctctg gcaccgtgat ccgcaccatg gatgacctgg ccgcccgcga actgccgcag
     2221 ggcatggaca cgctgtggac cgagctgacg ctgctgcaaa tccgggccgg caacacggcg
     2281 atcatcgtct tcgccgtagc cgtggtgctg gtcttcctcg tgctcgcggc ccagtacgaa
     2341 agcctgacgc tgccgctggc ggtgattctc gtcgtgccga tgtgcctgct ctgctcggtc
     2401 gtcggcgtgg ccctggccgg catggacatc aacatcttcg tgcaaatcgg cttcgtcgtg
     2461 ctcgtcggcc tggccagcaa aaacgcgatc ctgatcgtcg aattcgccaa ggagaaatcc
     2521 gcccacggga tggcgcccgc cgacgccacc gtcgaggcct gccgcctgcg gctgcggccg
     2581 atcatcatga cctcgctggc cttcatcctc ggcgtcgtgc cgctagcact ggccgtcggg
     2641 gccggcgccg agatgcgcag cacgctgggc atcgccgtgt tctccggcat gctcggcgtg
     2701 accctgtttg gcatcttcct gacgcccgtc ttctactacg tgatcgtgtc gctgttcggt
     2761 cagcccgcgg cagccccggc cgccagcggc gatccaccgg gagaggacac ggcacatccc
     2821 gccaaggctg cgtgacgttg cgccgagggc ctgcctgcga cctaggccca gcccaggact
     2881 cgcgccgtgt gccgcgcgat catcagctcc tcgttggtgg gaatcaccca ggccgagacc
     2941 cggctctgcg gcgcggtgat ccgcgggccg ccggaggcgt tggcctgggc atcgagggcc
     3001 agtcccaacc aggccgacga ctcgcagatt cgccggcgaa tctccgccga gttctcgccg
     3061 atgccggccg tgaacaccat cgcatcgatc ccgcccagca ccgccgccag cgcgccgatt
     3121 tctttggcgg cccggtaaac aaaatagtcc acggccagcc gggcctcggg ctcgtcgcgc
     3181 gcgagcaggt cgcgcatgtc gctgctgatc ccggaaatac cgagcaggcc cgacttcttg
     3241 tagagcagcg tctcgacttc cgccgccgag aactgcagac tttgaaacag gtgcagcaca
     3301 ataccgggat cgagcgcgcc gggccgggtg cccatgcaca ggccatcgag ggcggtaaac
     3361 ccgagcgtgt ggtcgatgct ctttcccccg cgcagcgcgc agaggctcgc accgcttccc
     3421 aggtgggcga caatcacccg gccgccggca atctccggcg cgaactgcgg caatgcggag
     3481 gcgatgtact cgtaagatag gccgtgaaag ccgtagcgct gcagacctgc ctcgcgcaat
     3541 tcgcgcggga gcggaacgag ttcggtcacc gccggccggc cgcggtgaaa gctggtgtcg
     3601 aaacaggcca cctgcggcac gtcgggcaat cgctcgccga tggcttcgat ggcggccagg
     3661 ttgtacggct gatgcagcgg cgcgagggga atcagctggc gcagttctgc caggacatcc
     3721 gggctgacga tcgtcggccc agcgaaccgc ggcccgccgt ggaccacacg atggccaacg
     3781 cccagcacgc gcgtaccgcc gtatttcgac ctcagccagg cggcgacggc agctacggcc
     3841 tcgcgggcgc cctgaaccgc aacctcctgc cgggccaggc tttcgccggc ggcgtccttg
     3901 accgtcaggc gcggcgcagt gccgatgcct tcgatctggc cccgcgcctc cactggccag
     3961 ccgccgctcg ccggccgctg aaagaccgtg aacttcaggc tcgacgagcc ggcgttgagc
     4021 accagcgcgt agtcgtccat gccgcgctcc tacggctttt tccgccgcgc atgagccacg
     4081 agcatcgcca cgccgcagct ggcgattcgg gaccgcacgc tatcggcccg gctggtgagg
     4141 atgataggga cccgcgcgcc caggaccagg cccgcgctgt cggcattggc caggaagctg
     4201 agctgcttgg ccaggatgtt gccggcttcc aggtcgggcg cgagcaggat atcgggatcg
     4261 ccggcgaccg cggagcgaat tcccttgatc cgggcggctt cggcgctgat ggcgttgtcg
     4321 aacgccagcg gcccatccag aatgccgccc gtgatctgcc cgcgctccgc cattttgcag
     4381 agcgcagccg cgtcaaccgt ggccggcatt ttggaggtaa ccgtttccac cgcagcaagg
     4441 atcgcgactt tcggctgcgc caggccgagg gaaatcgcca ggtcgatggc gttctggcag
     4501 atgtcggcct tgtcctccaa ggcaggagca atattgatcg cgccgtcagt cacgatcagc
     4561 accttgtgat aggtggggac gtccatcaca aacacgtggc tgatgcggcg cgccgtgcgc
     4621 aggccgccgt cgcgggccac gaccgccgcc atcagctcgt cggtgtgcag gctgcccttc
     4681 atcagcattt cggcctgccc ttcgcggacc agctcgacgg ccttggccgc cgcggcatgg
     4741 ctgtgcggga cgtcgatgat ggggagtgtt tcgagatcga tgccggccgc cgcggccgcc
     4801 gcggtgatct tggcccgcgg cccgaccagg aggggcgcaa gcaatccgag cttccaggct
     4861 tgggccgctc ccgacagcgc cgctgcgtcg caaggatgga ccacggccgc tggcaccggc
     4921 ggcaggctct tgcaccgcgc caggagctgt tcgtacttgc cggttcccgt ttcaggcttc
     4981 atttccagag cgctcctcag ccagccgtgg ggcgagcgcc cgcggacagc ttctcgagga
     5041 gttgagtcag cgtgcggcgg acggaatcga tcgtgtaggg atcctcttcg tgcggcggac
     5101 tgccctcttt ctcgaagtag ggccgctcgc catacacccg cccccattcc acgtcggatt
     5161 cccggaccag ctcctggagc agatactcgg agtccagcac gcgctggatg tagcccgcca
     5221 gcacaaccag cggctgttcg aggttgttca gcagcagctc gctttccctg acctggcgga
     5281 gcaggaccac cgagatagcg cccgcgggac cgacgaggtg gcgttccaga aaactctcga
     5341 tctccgcgtc ggcctgctgc ttgagcgtgg cgggcgacac gcccttcatc attcccggtc
     5401 cggccagccg cccgatggcc tcggccagcg agaacttgcg gccctcgcga atttcccgct
     5461 ccaggtcctc atccgcctgc gaacgccgtt tcggctgctc gtcgctcatg tgcctgaccc
     5521 tccaggcagt gcggtcggat tgcattgtat cacggcaatc gcggcaagag ctcacacagc
     5581 gacgcggagg cacagagccg aggactccaa ctccacctct ccgcgacttc gcgtgaaact
     5641 cgatcccgac tcattccggc cggtagccgc gccaggtcca gacgagcgtg tcggccagcg
     5701 tttgctcgaa gactttggag tcgcagtgcc gtgtggcctt gctgaaaacg tagcggtagt
     5761 cgtagccctt ggcctggagg gctgcggcgg tccgctcgtt ggccatcacc cagttgtgat
     5821 atgtttcttc ggggtccttc gcgcgattgt cattttcgga aacgtgcgtg aagattcgca
     5881 ggggcttctt ctcgctgttt t
//
LOCUS       DASRJB010000652         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2446223, whole genome shotgun sequence.
ACCESSION   DASRJB010000652 DASRJB010000000
VERSION     DASRJB010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446223"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFB80_17270"
     CDS             complement(<1..405)
                     /locus_tag="VFB80_17270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35585.1"
                     /translation="MSRSENRIRLSVVVPLPDHRGHALEAIRSWTQEQTFPHSEFEVV
                     VIIDGREPETEREVAALLAPHDRLVTVARGSLHDCYNAGRAAARGEALFFTESHVKAV
                     PDCVQQMIARLAAGDADCVAVASGGIDEDRFAA"
     gene            complement(395..1336)
                     /locus_tag="VFB80_17275"
     CDS             complement(395..1336)
                     /locus_tag="VFB80_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35586.1"
                     /translation="MSCILQLVPTADFPEERFAPRRERSAAYRAAVERGRAAMGRSRV
                     AICGLARNVEAYLPLTIARIERLGRMFADYRVFIYENDSTDATPRQLAQWAASNRRVE
                     VVSETRHLPRHPAVRSLERAADMAAYRDRCQCEVGRRWADFDYACVVDTDLPDGWSYD
                     GIAHSFGCGEWDFVGSYGIIYQRQRLIPSRALHYDVWAYRPLGSFEPLETREGNLLAW
                     RRGEALVPINSCFGGLGLYRMPAWLSAQYRGSDCEHVPLHREMRERGYGRLFLNPSQI
                     ALYGRKRKRFDSLVLSVDRFVRRWFGASRYEVAAHVA"
     gene            complement(1446..2621)
                     /locus_tag="VFB80_17280"
     CDS             complement(1446..2621)
                     /locus_tag="VFB80_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN35587.1"
                     /translation="MLSKPRKTTLTRGETLWSLVAHDGGDVMPLRSFPCAVGRHPGIA
                     VRIVHPTVSLNHAEFRRLGDKLVLADLGSRNGTFVNGQRIKGEHPIGSGDLAQFGGAV
                     FRLQNHHLPSELSPTCPGEDMGDLALALAQFEKLISEAAVVPVYQPIVEIEGNQVVAY
                     EALARSRLFGLDKPDLMFKAAGYFRMEAELSRLLRRIELIRSCPTNQPPLFMNTHPTE
                     LTDFKRLILSLREIRAVRPQQPLTLEVHEAAAADLSTMKMLRLALDDLHMTLAYDDFG
                     AGQARLAELAEARPEYVKFDRQLISGIDQPGRHRRQVVESLVDMCKQLGIVTLAEGVE
                     TASEAAACKDLGFELMQGYFCGRPAGAKITTGAVLTPEDDSELLASLQPPVEVAAAR"
     gene            2972..>3414
                     /locus_tag="VFB80_17285"
     CDS             2972..>3414
                     /locus_tag="VFB80_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35588.1"
                     /translation="MPAVLPSNWTADSRTPPIGADHSTSIAPYLQGISFFVAVVLGSA
                     WAVKGLWSVLRRDWEWLPPLSYGRSLSLVVLWGLLFVVVLTMISGARELMTPGAWKKD
                     GVTYSLDDPKKQAVPYVLPTEAERREKLKVLFAELAAYAVRHDGA"
BASE COUNT          563 a         1123 c         1160 g          568 t
ORIGIN      
        1 agcggcgaag cggtcttcgt cgatgccgcc gctggcgacc gccacgcagt cggcatcgcc
       61 cgccgccagc cgcgcgatca tttgctgcac gcaatccggt acggccttga cgtgcgattc
      121 ggtgaagaag agagcctccc cgcgtgcagc ggcgcggccg gcgttgtagc agtcgtgcag
      181 cgagccgcgg gcgaccgtca ccagccggtc gtgcggagcc aacagcgccg cgacctcgcg
      241 ctccgtttcc ggctcgcggc cgtcgatgat cacgacgact tcaaactcgc tgtgggggaa
      301 ggtctgctcc tgcgtccacg agcggatcgc ctccagggcg tgcccgcgat ggtcgggcag
      361 cggcaccacc acggagagcc ggatgcggtt ttcgctacgc gacatgggca gcgacctcat
      421 atcgcgatgc gccgaaccac cgccgcacga agcgatcgac cgacagcacc aggctgtcga
      481 accgctttcg cttgcggccg tagagggcga tctggctggg gttgagaaac agccggccgt
      541 agccccgctc gcgcatctcg cggtgcagcg gcacgtgctc gcagtcgctg ccgcggtatt
      601 gggcgctcag ccaggcgggc atccggtaca gtcccagccc gccgaagcac gagttgatcg
      661 gcacgagcgc ctcgcctcgc cgccaggcca gcaggttgcc ctcgcgcgtc tccagcggct
      721 cgaagctgcc cagcgggcga taggcccaga cgtcgtagtg cagcgcccgc gagggtatca
      781 gccgctgccg ctggtagatg atgccatagg aaccgacgaa gtcccactcg ccgcagccga
      841 agctgtgggc gatgccgtcg tagctccagc cgtcgggcag gtcggtatcg acgacgcagg
      901 cgtagtcgaa atcggcccag cgccgaccga cttcgcactg gcagcggtcg cgataggcgg
      961 ccatgtcggc ggcccgctcg agcgaccgga ccgccggatg ccgcggcaag tggcgggttt
     1021 cgctgacgac ctccacacgg cgattggagg cagcccactg agcaagctgc cgcggcgtgg
     1081 cgtccgtcga gtcgttttcg taaatgaaga cgcggtagtc ggcgaacatc cgccccagcc
     1141 gctcgatgcg ggcgatcgtc agcggcaggt aagcctcgac gtttcgcgcc aggccgcaga
     1201 tggcgacgcg cgagcggccc attgcggcgc ggccgcgctc gacggccgcc cggtaagccg
     1261 cggaccgctc gcgcctgggc gcgaaccgct cttccggaaa atccgccgtg ggcaccaatt
     1321 gcaagatgca ggacatgcta gcagcgtgat cgcagcccca ggacagagcc tgccggtcag
     1381 gcaagcccgt caagacgtat cggttgcgca agagggccag cttgaagcga agaatgggga
     1441 ggcgctcacc gcgccgcagc gacttcgacg ggcggttgca gggatgccag cagctccgag
     1501 tcatcctcgg gagtcaggac tgcgcccgtc gtgatcttcg cccccgccgg ccgaccgcaa
     1561 aaatagccct gcatcagctc gaagcccaga tccttgcagg ccgccgcttc gctcgcggtc
     1621 tcgacgcctt cggccagcgt cacgatgccc agctgcttgc acatatcgac cagcgactcg
     1681 accacttgcc gccggtgccg tcccggctga tcgatcccgc tgatcagctg gcggtcgaat
     1741 ttcacgtact ccgggcgagc ctcggccagt tcggccagcc gcgcttggcc cgcgccgaag
     1801 tcgtcgtagg ccagcgtcat gtgcaggtcg tcgagcgcca gccgcagcat cttcatcgtg
     1861 ctcaggtcgg ccgcggcggc ctcgtgcacc tccagcgtca gcggctgctg tgggcggacg
     1921 gctcgaatct cgcgcagcga caggatcagc cgcttgaaat cggtcaactc cgtcgggtgc
     1981 gtgttcatga acaggggcgg ctggttcgtc gggcaggaac ggatgagctc gatccgccgc
     2041 aagagccggc tcagctcggc ctccatccgg aaatagccgg cggctttgaa catcaggtcg
     2101 ggcttgtcga ggccgaacaa ccggctgcgg gccagcgcct cgtaagccac cacctggttg
     2161 ccttcaatct cgacgatcgg ctgataaacc ggaacgacgg ctgcctcgct gatcagtttt
     2221 tcgaactgtg ccagggcgag ggccaggtcg cccatgtctt cgccggggca ggtgggcgag
     2281 agctcgctcg gcaggtggtg gttctgcagg cggaacaccg cgccgccgaa ctgggccagg
     2341 tcgccgctgc cgatagggtg ctcgcccttg atccgctggc cgttgacaaa ggtgccgttg
     2401 cgactgccca gatcggccag gacgagcttg tcgccaaggc ggcggaactc ggcgtggttc
     2461 agtgacaccg tgggatggac aatgcggacc gcgatgcccg gatgccggcc gaccgcacag
     2521 ggaaacgacc gcagcggcat cacatcgccg ccgtcgtgtg cgacgagcga ccagagggtc
     2581 tcgccgcgag tcagcgtcgt tttgcggggc ttggaaagca tcggcgatct ccgctgggtt
     2641 gccccggcgc ggccgcctgc ggcagccgtc cacggcgttt cccccgaaag attagctcag
     2701 aacaacccca tggcatggct ttgttgcgac tccctggcag gcggtggtcc gcctgtagcg
     2761 attgtgcaat cgcacgaaat ttgtcacacc accaagcggg tggtgaattg aagaattgct
     2821 ggcgggtctc aggcgaacag gcgtaaaatg accgctccca ttccatcttc catggggtgt
     2881 acttgagttc gcattcatgc accgttcgcg gctaatcctc gtttgcacct tggccttgct
     2941 ggtcctgact tgcggttcgg cgacagcggg gatgccagcc gtcttgccgt cgaactggac
     3001 cgccgacagc cgaacgccgc cgattggcgc ggaccattcg acgagcatcg cgccgtatct
     3061 ccaggggatt tcgttcttcg tcgcagtcgt cctgggctcg gcttgggcgg tgaagggatt
     3121 gtggagcgtg ctgcggcgcg attgggagtg gctgcccccg ctgagctatg gccggtcgct
     3181 gagcctagtc gtgctatggg gcctgctatt cgtcgttgtg ctgacgatga tctccggcgc
     3241 gcgtgaattg atgacgcccg gcgcgtggaa gaaggacggg gtgacgtact cgctcgacga
     3301 cccgaagaag caagcggtgc cctacgtcct cccgaccgag gcggagcgcc gcgagaaact
     3361 gaaggtgctg ttcgccgagt tggccgccta cgcggtgcgg cacgacgggg cgta
//
LOCUS       DASRJB010000653         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3855934, whole genome shotgun sequence.
ACCESSION   DASRJB010000653 DASRJB010000000
VERSION     DASRJB010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3855934"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFB80_17290"
     CDS             complement(<1..948)
                     /locus_tag="VFB80_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35589.1"
                     /translation="MPLKVIHDEFQPSWEEEGLFARDVNGQLVRVVEASEQDYFQQVT
                     VTIDGQAIRVPRAVPATDAQGNIIYLDAAGRTQPRRTTIYDAALALGLADLRKRRGQA
                     AERELLDDAKSRLLRTIPTLCHLDHLAPAGVCRVCSVELAREETDPKDPAKKVYRGSG
                     KLVPACVQPVEDRMFVNTLAGADGQRVRRAVKVLLELLAADHLPAQAATAGQPPTELA
                     RLIFELNRTVLSLDAEARQRGGWLTPERFAPQSPPVSACDESSPLIRVDPAACILCDR
                     CARACNDVKQNFVIGRTGKGYGTRIGFDLNDPMGDSSCVE"
     gene            complement(981..2933)
                     /locus_tag="VFB80_17295"
     CDS             complement(981..2933)
                     /locus_tag="VFB80_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZN35590.1"
                     /translation="MIVQQLNAIQERHGYLPREALVSLADRLAVPLYRIQEVVSFYPH
                     YRRQPPPAVEVHVCRDMSCCLRGSAKLTGTLEQSLAKEISGGRAAVCPVSCLGRCDRA
                     PAARLDHNAASHQIHNYFGRAPDDIVRAVQDLLAGETEPSIAADRDADLPDRSSDWKI
                     NVYAGQPREERYRALAQVAAALRRGAAAGDAERDRIIQAALKNAVLLGMGGAGGQAFK
                     KWCEVREAVGERKYVVCNADESEPGTFKDRELLLRTPYLVVEGVLLAGLVLGAEQGYI
                     YIRHEYEECIAAVREEIAWARQAGLCGDDILGCGAAMHVDVFVSPGGYICGEQTALIQ
                     AIEDKRAEPRNRPPELQTNGLWDLPTLVNNVETLAWAPAVILLGEAEAAAAPGARARQ
                     TCRAMEKAGPETDRSWYGRQGRPFGELAQAILGSDADWQRRHGSAARFPGRRMFALSG
                     DVHSPGVYEVENGTTVRELLALAGGIRGARPLLAIALSGPSGGFTPAVLPRAAWPRKI
                     QKLLPESIQQVELLDLQMHINYFRVWDLMLGAGIVVYAAGADLVAQALACQRFFQAES
                     CGKCVPCRIGSTKLAELTEQLAAGRLSAEQIIQLQGETGPIPELALTMASTAICGLGT
                     VAPNPLTSLLRHFPKLVAQASSLVAH"
     gene            complement(2930..>4141)
                     /locus_tag="VFB80_17300"
     CDS             complement(2930..>4141)
                     /locus_tag="VFB80_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35591.1"
                     /translation="YHHGEQAARADVWRAAKIRPVEFQAVGGFADHSHSKLPLVASCS
                     ASGCHGDARAGAPPWRSAQAVWLAADPHASAYDVLWTFRGREITRRLAQPTGALTDEQ
                     HRQAIEQRCIGCHATTRDEGGFTLGVHCESCHGQADEWLKSHQLTSFSRGTHGFIDTK
                     SLPARAGVCMPCHVGPQVSGQTTQAVDHDLIAAGHPRLNFEFRTYFESLPEHWDRQSD
                     QRRLGPDFHFRSWLAGQQQQAAQFTRLRTHFGQTSPDDVREFALFDCASCHHALATGE
                     ATAGRPRPPHWPRGDLPEAQPLAVAGSLLAEAAIVNRNTFDDNVQTYLALQAVAADLP
                     ARPALHSTIDRLGQFLAQDCFFAAGDSRPPTQYDWPRRLDRSQLDLRIQAIRAVLQSL
                     QNPPAGGLAPP"
BASE COUNT          626 a         1404 c         1450 g          661 t
ORIGIN      
        1 ctcgacgcac gacgagtccc ccatcggatc gttcaggtcg aaaccgatcc gcgtgccgta
       61 gccctttccg gtgcggccga tgacgaagtt ctgcttcacg tcgttgcagg cccgggcgca
      121 gcggtcgcag agaatgcagg cggccggatc gacgcggatc agcggcgagg actcgtcgca
      181 ggcgctcacg ggcggcgact gcggcgcgaa ccgctcgggc gtcagccacc cgccgcgctg
      241 gcgggcctcg gcgtcgagcg acagcaccgt gcggttcagc tcgaagatca gtcgggccag
      301 ctcggtcggc ggctggcctg cggtcgcagc ttgagccggc aggtggtcgg ctgccagcag
      361 ctccagcagt accttcaccg cgcggcggac ccgctgcccg tcggcgccgg ccagcgtgtt
      421 gacgaacatc cgatcttcca ccggctgcac gcacgccggc acgagcttgc cgctgccgcg
      481 atagaccttt ttcgccgggt ccttggggtc ggtttcctcg cgggccagtt cgaccgagca
      541 gacgcggcaa acgcccgccg gcgcgaggtg atcgaggtgg cacagggtgg gaatcgtccg
      601 cagcagccgc gacttcgcgt cgtccagcag ttcccgctcc gccgcctggc cgcggcgctt
      661 gcggaggtcg gccaggccca gggccagggc cgcgtcgtag atcgtcgtcc gccgcggctg
      721 cgtccgcccg gcggcgtcga gatagatgat gttgccctgg gcgtccgtgg ccggcaccgc
      781 ccgcggcacg cggatcgctt gcccgtcgat cgtcaccgtc acctgctgga aatagtcctg
      841 ctcgctggcc tcgacgacgc ggacgagctg accgttgacg tcgcgggcga agaggccttc
      901 ttcctcccag ctcggctgaa actcgtcgtg gatgactttg aggggcatag gtgcttcgta
      961 gagcaagcgt cctcgcttgc tcaatgggcc acaagcgagg acgcttgtgc tacgagtttg
     1021 ggaaagtgcc gcagcaggct agtcagcgga ttgggagcga cggtgcccag gccgcagatg
     1081 gcggtgctcg ccatggtgag agcgagctca gggatcgggc cggtttcgcc ttgcaactgg
     1141 atgatctgct cggcactcaa gcggccggcg gccaactgct ctgtcagctc ggccaacttg
     1201 gtcgagccaa tgcggcaagg gacgcacttg ccgcaggact cggcctggaa gaatcgctgg
     1261 caggccaggg cctgcgccac caggtccgcg ccggccgcat agacgacgat ccccgctccc
     1321 agcatcagat cccagacccg gaaatagttg atgtgcatct gcagatcgag cagctcgacc
     1381 tgctgaatcg attcgggcag cagcttctgg atcttccgcg gccaggcggc ccgcggcagt
     1441 acagccggcg tgaagccgcc ggaagggccg gagagcgcga tggcgagcag cggcctagcg
     1501 ccgcgaatgc cgccggccag tgcgagcagc tcgcggacgg tggtgccgtt ttcgacttca
     1561 tacacgcctg gcgagtggac gtcgccggag agcgcgaaca tccgccggcc gggaaaccgc
     1621 gccgccgacc cgtgccgccg ctgccaatcc gcatcgctgc cgaggatcgc ctgggccagt
     1681 tcaccgaacg gccggccctg gcggccgtac cagctgcggt cggtttcggg cccggccttt
     1741 tccatcgcgc ggcaagtttg acgcgcgcga gcgccgggcg ctgccgccgc ctccgcctcg
     1801 cccagcagga tcaccgccgg cgcccaagcc agcgtctcga cgttgttgac cagcgtgggc
     1861 aaatcccaca ggccgttggt ctgcagttcc ggcgggcggt tgcgcggctc ggcccgcttg
     1921 tcctcgatcg cctggatcag ggccgtctgc tcgccgcaga tgtagccgcc ggggctgacg
     1981 aagacgtcga cgtgcatcgc ggccccgcag ccgaggatat cgtcgccgca gaggcccgcc
     2041 tgcctggccc aggcgatttc ctcgcgcacg gcggcaatgc actcttcgta ttcgtggcgg
     2101 atgtagatgt agccttgctc cgcgcccagg acgagtccgg ccagaagcac gccttcgacc
     2161 acgaggtagg gcgtcctgag cagcagctcg cggtctttga acgtgccggg ctcgctttcg
     2221 tcggcgttgc agacgacata cttccgctcg ccgacggcct cgcggacctc gcaccacttc
     2281 ttgaaggcct gcccgcccgc gccgcccatc cccagcagca cggcgttctt cagtgcggcc
     2341 tgaatgatcc ggtcgcgctc cgcatcgccc gccgccgcgc cgcgccgcag cgcggccgcc
     2401 acctgcgcca gtgcccggta gcgctcctcc cgcggctgcc cggcatagac gttgatcttc
     2461 cagtcgctgg accgatcggg caggtcggcg tcgcgatcgg cggcgatcga cggctccgtt
     2521 tctccggcca gcaggtcctg gacggcacgg acgatatcgt cgggcgcgcg gccgaagtag
     2581 ttgtggattt gatgagatgc cgcgttgtga tcgaggcgcg ccgccggtgc gcggtcgcag
     2641 cgccccaggc aagacacggg gcagaccgcg gcgcggccgc cggagatctc tttggccagc
     2701 gattgctcca gcgtgcccgt cagcttcgcc gagccgcgga gacagcagct catgtcgcgg
     2761 cagacgtgca cttcaactgc cggcggcggc tggcggcgat agtgcggata gaagctcact
     2821 acttcttgaa tgcggtagag cggcaccgcc agccgatcgg ccagcgacac gagggcctcc
     2881 cgcggcagat agccgtggcg ctcctggatc gcattgagct gctggacgat cacggcggcg
     2941 cgaggcctcc tgccggcggg ttttgcagcg actggagtac cgcgcggatc gcctggatcc
     3001 gcaaatccag ctgcgagcgg tccagccgcc gcggccaatc gtactgcgtc ggcggtcggg
     3061 agtcgcccgc agcgaaaaag cagtcctgag cgaggaattg gcccagccgg tcgatcgtgg
     3121 aatgcagagc aggccgcgcc ggcaggtccg ctgccaccgc ctgaagcgcc aggtaggtct
     3181 ggacattgtc gtcgaacgtg ttgcgattca cgatggccgc ttccgcgagc agcgaaccgg
     3241 ccacggccag cggctgggcc tcgggcagat cgccgcgcgg ccagtgcggc gggcgcggcc
     3301 tgcccgcggt agcctcgcca gtcgccagcg cgtgatggca cgaggcgcaa tcgaacaagg
     3361 cgaactcgcg cacatcgtcc ggagaggtct ggccgaagtg cgtccttagc cgggtgaact
     3421 gtgccgcctg ttgctgctgg ccggcgagcc agctccggaa atgaaagtca gggcccagcc
     3481 ggcgctggtc ggattgtcga tcccagtgtt caggcaggct ctcgaagtac gtgcggaatt
     3541 cgaagttcag ccgcggatgg ccggcggcaa tcaggtcgtg atcgacggct tgggtggtct
     3601 gaccggaaac ttgcggtccg acgtggcaag gcatgcagac gcccgcccgc gcgggcaaac
     3661 tcttcgtgtc gatgaagccg tgcgtgccgc gcgagaagct cgtcagctgg tgcgacttaa
     3721 gccactcgtc ggcttggccg tggcacgact cgcaatgtac tcccagagtg aagccgcctt
     3781 catcgcgtgt ggtggcgtgg cagccgatgc agcgctgctc gatggcttgg cgatgctgct
     3841 cgtcggttag cgcgcctgtt ggctgcgcga gccggcgggt gatctcgcgg cctcggaacg
     3901 tccagaggac gtcgtaggcc gaagcgtgcg gatcggcggc cagccacacg gcctgcgcac
     3961 tgcgccaggg cggcgcgccc gcgcgggcgt cgccgtggca gccgctggcg gaacaactgg
     4021 cgacaagagg cagtttcgag tggctgtggt cggcgaagcc gcccacagcc tggaattcca
     4081 cagggcggat cttcgccgca cgccacacgt cggccctcgc cgcttgctcc ccgtgatgat
     4141 a
//
LOCUS       DASRJB010000654         9611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3292828, whole genome shotgun sequence.
ACCESSION   DASRJB010000654 DASRJB010000000
VERSION     DASRJB010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292828"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFB80_17305"
     CDS             complement(<1..595)
                     /locus_tag="VFB80_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35592.1"
                     /translation="MLKLACKGTLNAVALAAAWPCALTVWLEGWLNPCREDAFVFWAQ
                     LLSLAPGMPGMYLRRAFYAWTLEFCSLDSCVGFGAIFSNRQAVVESRVYIGPFALIGS
                     ARLRRGCLIGSRASLLSGGRLHAQSASGDWLPTDPALRQQIEIGENAWLGEGAIVMAD
                     IGPRAMVSAGAVVSTPVPERVMVAGNPARFVKRLAADD"
     gene            complement(595..1956)
                     /locus_tag="VFB80_17310"
     CDS             complement(595..1956)
                     /locus_tag="VFB80_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HZN35593.1"
                     /translation="MTDANTSPLEWLLERISFAGAASAVATGDSETPYHELAATVERW
                     RSRLLAAGVLAGDVVSIEGEYAAPTIAALLAAADLRAIVVPLSADMRVHHDRFRRIAQ
                     VQWRVSCAAEEIVRCESRAVHPHFERLRAGGRPGLVLFTSGSTGESKAAVHDLGLLLE
                     KFHVRRQSLRTLVFLQLDHIGGVNTLLHTLANGGAVIVPADRSPAAVCRAIERHRVEL
                     LPASPTFLNLLLLSEEPARRDLSSLRLITYGTEPMPPSTLSRLHAAFPGVKLQQTYGL
                     TELGILRSQSRGSDSLWVRVGGEGYETKVVDGRLWIRARSGMLGYLNAESPFDAEGFF
                     DTGDRVEVDGPWLRILGRQSEVINVGGSKVDPAAVESVLLEMDGVAEAAVRGEPHPIT
                     GQIVAATVRLARDESPRDFRVRMRSYCRDRLPDYAVPARVSFGSEPLHSVRFKCIRRG
                     VDA"
     gene            complement(1972..2676)
                     /locus_tag="VFB80_17315"
     CDS             complement(1972..2676)
                     /locus_tag="VFB80_17315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN35594.1"
                     /translation="MNPPRHILITGASRGLGRALAEHYVERGDAVVGCSRGPATLEHP
                     QYSHVAADVTDEAAVQRLCDQVRERCGGLDVLLNNAGVGRMLPVALTPLATARRLVDV
                     NFLGTFLLTHAAVRLLRHSPAGRIVNFTSIAVPLRLEGEAIYAAAKSAVETFTRVVAK
                     ELGPLGITCNAVGPSPIRTDLLRNVPEEKLQALIDRQAIRTWAEPQDVINIVDFFLKP
                     ESRHVTGQVLYLGGLG"
     gene            complement(2673..2969)
                     /locus_tag="VFB80_17320"
     CDS             complement(2673..2969)
                     /locus_tag="VFB80_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35595.1"
                     /translation="MDRHQIEDVVVSAIRIINQGRTPEHQLAESADAALYGAGSPLDS
                     LGLVALLIEVEEGLREEGRAISLSDERAVSQKHSPFRSVPALVDYIAGRLGNPA"
     gene            complement(2974..3621)
                     /locus_tag="VFB80_17325"
     CDS             complement(2974..3621)
                     /locus_tag="VFB80_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35596.1"
                     /translation="MSDVALDTPSVVRVEQPRRSALFVGLKRCVQAAFFVWALPRLIA
                     YWLSRRVLGRRAFGAASESIARVSGIRGLFLRQAFYRATLSACGRDAYFGWLSVFSMP
                     EASVGERAYIGRFCTIGFAEIGEEAMLADHVQILSGGQEHGQAAGSSMHEQPQTYSRV
                     RIGRGAWIGAGAVVMADVGAGAIVGAGAVVTRPVPPRSVAVGVPAKVIKSLDETN"
     gene            complement(3618..4790)
                     /locus_tag="VFB80_17330"
     CDS             complement(3618..4790)
                     /locus_tag="VFB80_17330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35597.1"
                     /translation="MTAASPLILHVRCVTGAGGGPEKTILNSPRFLARLGYDCACAYL
                     HPPDDPGFDVLRRRADAAEARLVGIPDRGPLDLGVVRRMARLCREERVAVWHGHDYKS
                     NALGLLVRWYWPMKLATTVHGWVQHTRRTPLYYAIDRFCLKRYDEILCVSQDLYDQCL
                     RLGVRPERCHWVHNGIDTEQFTRRQKAAAARRAFSRHVSGQLIGAMGRLSDEKGFDLL
                     IDAVDRLLNEGYDISLWIAGEGAQRASLQKLIDDRGQSSRIQLLGHVEDTQSFYEALD
                     LFALSSRREGLPNVLLEAMALEVPVVATRIAGVPTLVEDGVSGLLVEPGDSVVLAGAL
                     RQVLGDAALRDCFAAAARQTIETTFSFERRMRRVAGIYDALLARKPAAAQAAAHHA"
     gene            complement(4799..6022)
                     /locus_tag="VFB80_17335"
     CDS             complement(4799..6022)
                     /locus_tag="VFB80_17335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35598.1"
                     /translation="MKILLLTEQFPWPLTDGGNLRTWHIIQGLQRQHEVTVLAHHWPP
                     RAGKLDQSPCGISRPVLVPKLDVSRRLWHNIRHLGPSRWPLFLLKNFSPTLLGEADRL
                     LASESFDVIHFNHLDTACFALTRDWPQPMVFDTHNCLTSLARQVAGAGANPVRRWFFR
                     REAALIEEAERRITARMALNLVCSSADAGHFMGIQPQGLYEVVPNGVDREHFQASAAS
                     PADPATLVFTGAMSYYPNVQAAVFFQREILPFLKRSGRPLRTLFVGRDPAPQVRRLHD
                     GERVIVTGSVPDVRPYLEQAAVVIVPLLHGSGTRLKILEAFSMGKAVVSTRVGAEGLG
                     VQNGKQLLLADEPRAFADCILRLLDDEGLRQRMGLAARQLVEAEYAWPVVQDRLNRAY
                     SQLCSTPAVETSAAI"
     gene            complement(6019..7194)
                     /locus_tag="VFB80_17340"
     CDS             complement(6019..7194)
                     /locus_tag="VFB80_17340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35599.1"
                     /translation="MTTATMLGALMPVRRPPALDDGRPVICQVLHTLHVGGGEVLARA
                     FALQHERDFRPVFALLDDIGSLGQELKDAGYTVEVISRRPGFDWRCGRRLRDFFRRHR
                     VALIHAHQYAPLFYSALARLPGMPLPILFTEHGRDYPDYRRWKRALANRFLFGRRDRF
                     VGVGQCVRRALIDYEGLPADRVEVIYNGSDLAAYDPLHGERDTVRRELGLADDEIAIV
                     QVARLNSLKDHATAIRAMQRLRSAAPRARLLLAGEGEERPAIERLIGELGVSDCVRLL
                     GTRRDVPRLLQGAEIFLLTSVSEGIPLTLIEAMATGLPCVATRVGGVPEVVQDRHTGR
                     LAAAQDHAAIAGLLAELAAQPAQRQALGKAGLERARQLFDHGQMHARYGSAYRQMIA"
     gene            complement(7191..>9611)
                     /locus_tag="VFB80_17345"
     CDS             complement(7191..>9611)
                     /locus_tag="VFB80_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35600.1"
                     /translation="AGDGPWELLLNDQDYSGELPWPVSVAQSSDAEIVVHTGKVRCRL
                     RRGDARILSSASSDSGENLGNKGVAICCIDARGARRRVTIESLRIVESGPLRAQVEFT
                     GVLGRGTDLRLRGHISFFAGLTQVRIELTAQNPRRARHPGGYWDLGDPGSVLVKQWSV
                     EIEANVGNQPRVAWTEQPGSEAQEVSGRLEILQESSGGKQWNSRNHVNREGRVPLRFR
                     GYSFRTADGECGGERASPAVAIQGAAGQVACAIEEFWQKFPSGLVVDENRISAQLWPG
                     SWGDLHELQAGEHCTRTVWLDFGRNAAAPCDGLSWVHRPPRAIADPEWLAASGAIPLS
                     PSPQAPVREEYQLVLREAADGPHSLLGKRERIDEYGWRNFGDVWADHEQAFGDDPRPV
                     ISHYNNQYDLLHSLLIHYLLSGDARWWALADPLARHVLDIDIYHTTRDKPAYNGGLFW
                     HTAHYHDAGKSSHRCVSAAMQGKKIPAPGTGPCNEHNYTRGLSLYYYLTGCERARRAV
                     LSMADWVLAMDDGCRHLLGILSSQPTGDASRTCETNYHGPGRGAGNSIHALLDAWQLT
                     GESHYLAKAAELVRRCIHPADDIAARDLASAERRWSYAVFLQALVRLIDAIGDVPEWD
                     LLRDYARQSLLHYARWMAAHERFYLDEPEKLEYPTETWAAQELRKGNTLLMAARYAAG
                     AEAARFRQRGREMLDRAWQSLLSFESRRTTRPVAVAMQQGYLEAYLQSEDRAPALAPG
                     GEDNREIGEPAVFMPQKQAIRHALRSPRGLLSLIGCAVQPGRWYSTARQTWPAERLRR
                     LLGASWLP"
BASE COUNT         1641 a         3294 c         3176 g         1500 t
ORIGIN      
        1 aatcgtcggc ggccagccgc ttgacgaacc gtgccggatt tccggcgacc atgacccgct
       61 ccggtacagg agtggagacg accgcgcccg ccgaaaccat cgctcgcggg ccgatatcgg
      121 ccatgacgat cgccccctcg cccagccagg cgttttcgcc gatctcgatc tgctgccgca
      181 gcgccggatc ggtcggcagc cagtcaccgc tggcgctttg cgcgtgcagc cggccgccgc
      241 tgagcaggct ggcccggctg ccaatcaggc atccgcgccg cagcctggcc gagccgatca
      301 aggcaaacgg cccgatgtac acgcgcgatt cgaccaccgc ttgtcgattg gaaaagatcg
      361 ccccgaatcc gacgcacgag tcgagcgaac agaattcgag ggtccaggca taaaatgcgc
      421 gccgcaaata catgcccggc atgcccggcg caagcgaaag caactgcgcc cagaagacga
      481 acgcatcttc gcggcacgga ttcagccagc cctcgagcca gaccgtcagc gcgcagggcc
      541 acgcagcggc cagcgccacg gcattcaagg tgcctttgca agccaacttc agcatcaggc
      601 gtccaccccg cggcgaatgc atttgaatcg cacgctatgc agcggctcgc tgccaaacga
      661 aacgcgagcc ggaacggcgt aatccggcag ccgatcgcgg cagtacgatc gcattcgcac
      721 ccggaagtcg cgcggcgatt cgtcccgcgc cagccgcacc gttgccgcga cgatctggcc
      781 cgtgatcggg tggggctcgc cgcgcacggc tgcctcggcg acgccatcca tttccaggag
      841 cacgctttcc actgcggctg gatcgacctt gctgccgccg acgttgatga cttcgctctg
      901 ccggcccagg atccgcagcc acgggccatc gacttcaacg cgatcgcccg tgtcgaaaaa
      961 gccctcggcg tcgaaggggc tctcggcgtt cagatagccg agcattcccg agcgggcgcg
     1021 aatccacagc cggccgtcca cgactttcgt ttcgtaccct tcgccgccga cgcggaccca
     1081 cagcgagtcc gagccgcgcg actgcgagcg gagaattccg agttcggtca agccatacgt
     1141 ctgctggagc ttgacgcccg gaaaggccgc gtgcagccgg gacagcgtgc tcggcggcat
     1201 cggctcagtg ccgtacgtga tgagccgcag cgaactcagg tcgcgtcggg ccggctcttc
     1261 ggagagcagc agcaggttga gaaacgtggg cgaggcaggc agcagctcca cgcggtggcg
     1321 ctcgatcgcc cggcagacgg ctgccggcga gcggtccgcg ggaacgataa cggccccgcc
     1381 gttggccagc gtgtggagca gcgtgtttac gccgccgatg tggtcgagtt gaagaaagac
     1441 gagcgtccgc agcgattgcc ggcggacgtg aaacttctcc agcaagaggc ccaggtcgtg
     1501 gacggcggcc ttgctttccc ccgtagagcc cgaggtgaac aggacgagtc ccggccggcc
     1561 tcccgcccgc agccgctcga agtgcggatg caccgcgcgg gactcgcagc ggacgatttc
     1621 ctcggccgcg cacgatactc gccactggac ctgcgcgatc ctgcggaagc gatcgtgatg
     1681 cacgcgcatg tcggccgaga gcggaacgac gatcgcccgc aggtccgcgg ctgccagcag
     1741 cgcggcgatc gtcggcgcgg cgtattctcc ctcgatcgag acgacgtcgc ccgcaagcac
     1801 gccggccgcc aacagccgcg accgccagcg ctcgaccgtg gcggccagct cgtgataggg
     1861 cgtctcggaa tcgcccgtcg cgacggcact cgctgcgccg gcgaacgaaa tccgctccag
     1921 cagccactcg agcggcgacg tgtttgcgtc cgtcataggg ggccgagtca tctagcccag
     1981 cccgcccagg taaagcacct ggcccgtgac gtggcggctt tcgggcttca ggaagaaatc
     2041 gacgatgttg atcacgtcct gcggctcggc ccaggtgcgg atagcttgcc ggtcgatcaa
     2101 ggcttgcagt ttctcttcgg gaacgttgcg gagcaggtcc gtgcggatcg gcgaggggcc
     2161 gacggcgttg caggtgatgc ccagcggtcc cagctccttg gccacgaccc gcgtaaaggt
     2221 ttccacggcc gacttggcag cggcatagat cgcttcgccc tccagacgca gcgggacggc
     2281 aatcgaagtg aagttcacga tgcggcccgc gggcgaatgg cgaagtaagc gaacggcagc
     2341 atgcgtgagc agaaaagtgc cgaggaaatt cacgtccacc agccgccgcg ccgtcgccag
     2401 cggcgtgagc gccaccggca gcatgcggcc gacgccggcg ttgttcagca gcacgtcgag
     2461 cccgccgcag cgctcgcgca cctggtcgca cagccgctgg accgcggcct cgtcggtgac
     2521 gtcggcggcg acatgcgaat actgcggatg ctcgagcgtg gccgggccgc gggagcagcc
     2581 gacgacggca tcgccccgct cgacgtagtg ttctgccagc gcgcggccca ggccgcgact
     2641 cgcgccggtg atcaggatgt ggcgcggcgg gttcatgcgg gatttcccag ccgcccggcg
     2701 atgtaatcga ccagtgccgg aacgctgcga aacgggctgt gcttttggga gaccgcccgc
     2761 tcgtcgctga gcgagattgc ccggccctcc tcgcgcagcc cttcctcgac ttcgatgagc
     2821 agggccacca ggccgagcga gtcgagcggg ctgcccgctc cgtacagcgc cgcgtccgcg
     2881 ctctcggcca gctggtgctc gggcgtgcgg ccctggttga tgatgcgtat cgccgacacg
     2941 acgacatctt cgatctggtg gcgatccatg gcctcagtta gtctcgtcga gcgatttaat
     3001 cactttggcc ggcacgccga ccgcgaccga ccgcggcgga accggcctgg tcacgaccgc
     3061 gcccgcgccg acgatcgccc ccgctcccac gtcggccatc accacggccc ccgcgccgat
     3121 ccacgctccg cggccgatcc gcacgcggct gtacgtttgc ggctgctcgt gcatggaact
     3181 gcctgcggcc tggccgtgct cctggccgcc gctgaggatt tgcacatgat cggccagcat
     3241 tgcttcctcg ccgatctccg caaagccgat ggtgcagaag cggccgatat aggcccgctc
     3301 gccgacgctg gcctcgggca tcgaaaagac gctgagccag ccgaaatagg catcgcgtcc
     3361 gcaggcggac aaggtcgccc ggtaaaaggc ctgccgcagg aagagcccgc ggataccgct
     3421 cacgcgggcg atcgactcgc tggccgcgcc gaaggcccgc cggcccagca ctcgccgcga
     3481 cagccaatag gcgatcagcc gcggcaatgc ccagacgaag aacgccgcct gcacgcagcg
     3541 cttgaggccg acgaagagtg cggagcggcg cggctgctcg acgcggacga cggacggagt
     3601 atccaaggcg acgtcgctca tgcgtggtgg gcggccgctt gagcggcggc cggcttgcgg
     3661 gcgagcaggg cgtcgtaaat gccggccacg cgccgcatcc gccgctcgaa actaaaggtc
     3721 gtctcgatcg tttgccgagc ggccgccgcg aagcagtcac gcaaggcggc gtcgccgagc
     3781 acttgccgca gggcgccggc cagaacgacg ctgtcgcccg gctcgacgag caggccgctc
     3841 acgccgtcct cgacgagcgt cggaacgccc gcaatccgcg tggcgacgac cggaacctcc
     3901 agcgccatcg cttcgagcag cacgttgggc aagccctccc gccggctgct cagcgcgaac
     3961 aggtcgagcg cctcatagaa cgactgcgtg tcctccacat gccccagcaa ttgaattcgc
     4021 gagctctggc cgcggtcgtc aatgagcttt tgcaaactcg cgcgctgggc gccttcaccc
     4081 gcgatccaca gcgaaatgtc atatccctcg ttcagcagcc gatcgacggc gtcgatcaac
     4141 aggtcgaacc ctttttcatc cgacaagcgt cccatcgcgc cgatgagctg cccgctgacg
     4201 tgccgcgaga acgcccggcg tgccgccgcg gccttctgcc gccgcgtgaa ttgctccgtg
     4261 tcgatcccgt tgtgcaccca gtggcaacgc tccggccgca cgccgagccg caggcactga
     4321 tcgtacaagt cctgcgaaac gcagaggatc tcgtcgtagc gcttgaggca gaaccgatcg
     4381 atcgcgtagt acagcggcgt gcggcgcgtg tgctgcaccc agccgtgcac agtcgtcgcg
     4441 agcttcatcg gccagtacca gcggacgagc aggcccaggg cattgctttt gtagtcgtgt
     4501 ccgtgccaga cggcgacccg ctcctcgcgg cacagccggg ccatccgccg gacaacgccc
     4561 agatccaagg gcccgcgatc ggggatgcca acgagcctag cctcagccgc gtccgcccgc
     4621 cgccgcagca cgtcgaaacc cggatcatcc ggcggatgca ggtaggcgca ggcgcagtcg
     4681 tatcccagcc gcgcgaggaa ccggggcgaa ttgaggatcg tcttctcggg gccgccaccg
     4741 gcgccggtga cgcaccgcac gtggaggatc aggggtgaag cggcagtcac gggaggcttt
     4801 agatcgcggc agacgtttcc acagcgggcg tggagcagag ttgcgaataa gcacggttca
     4861 agcggtcttg caccacgggc caggcatact cggcttccac cagttggcgg gctgccagac
     4921 ccatccgctg tcgcaggccc tcgtcgtcca gcaatcgcag aatgcagtcg gcgaacgccc
     4981 ggggctcgtc ggccagcagc aactgcttgc cgttttgcac gcccagaccc tcggccccaa
     5041 cgcgggtcga aacgaccgcc ttgcccatcg aaaatgcttc caggatcttg agccgcgtgc
     5101 cgctcccgtg gagcagcggc acgatcacca ctgccgcttg ctcgagatag ggccgcacat
     5161 cgggcaccga gcccgtgacg atgacgcgct cgccgtcgtg cagccttcgc acctgcggcg
     5221 ctggatcccg gccgacgaac agggtgcgca gcggacgtcc agagcgtttc aggaagggca
     5281 ggatttcgcg ctgaaagaag accgcggcct gcacgtttgg gtaatagctc atcgcgccgg
     5341 taaagaccag cgtggcggga tcagccggac tggcggccga agcctgaaaa tgctcgcgat
     5401 ccacaccgtt ggggacgacc tcatacagcc cctgcggctg aatgcccatg aagtgtccgg
     5461 cgtcggcgct cgagcagacg aggttgagcg ccatgcgggc cgtaatccgc cgctccgcct
     5521 cctcgatcaa cgccgcctcg cggcgaaaaa accagcggcg gaccgggttg gcgcctgcgc
     5581 cggccacctg gcgggccagc gacgtcagac aattgtgcgt gtcgaagacc atcggctgcg
     5641 gccagtcgcg agtcagcgca aagcaggccg tatccaggtg gttgaagtgg atgacgtcga
     5701 agctctcgct ggcgagcaac cggtcggctt cgcctagcag cgttggggaa aagttcttca
     5761 acaggaacag gggccagcgg gaaggaccga gatgacggat gttgtgccag agccgccggc
     5821 tcacatcgag cttcggcacc agcacaggac gggaaatgcc gcacggcgac tggtccaact
     5881 ttccggcccg cggcggccag tggtgcgcca gcacggtcac ctcgtgctgg cgctgcagcc
     5941 cctggatgat gtgccaggtg cgcaaattgc cgccgtccgt gagcggccac gggaattgct
     6001 ccgtgagcag caggatcttc atgcgatcat ttggcgatag gcgctgccat agcgggcgtg
     6061 catttgcccg tggtcgaaca gctgccgggc tcgctccagg ccggctttcc cgagggcttg
     6121 ccgctgcgcg ggttgggcag ccagctcggc cagcaacccg gcgatggctg cgtgatcttg
     6181 cgccgcggca agccgcccgg tgtgacggtc ttgcacgact tcgggcacgc cgccgacgcg
     6241 cgtcgcgacg cacggcaggc cggtagccat cgcttcgatc aaagtcaggg gaatgccttc
     6301 gctgacgctc gtcagcagaa agatctcggc cccttgcaac agccgcggca cgtcgcggcg
     6361 cgtaccgagc agccgcacgc aatcgctcac gcccaactcg ccgatcaatc gctcgatcgc
     6421 cggccgctct tcgccctcgc cggccaatag cagcctggcc cgcggagcgg ccgagcgcaa
     6481 gcgctgcatg gcgcggatgg ccgtggcgtg gtctttgagc gaattgagcc gggcgacttg
     6541 cacgatcgcg atttcgtcgt cggcgaggcc cagttcgcgg cgaacggtgt cgcgctcccc
     6601 gtggagggga tcgtacgccg cgaggtcgct gccgttatag atcacctcga cgcggtcggc
     6661 cggcagccct tcgtagtcga tgagcgcccg ccggacgcac tggccgactc cgacgaaacg
     6721 gtcgcggcgg ccgaacaaaa agcggttggc cagcgcccgc ttccagcggc ggtagtcggg
     6781 ataatcgcgg ccgtgctcgg tgaacaggat cggcagcggc atccctggca atcgggccag
     6841 tgccgaatag aacagaggag cgtactggtg ggcgtgaatc agggctacgc gatgccggcg
     6901 gaagaaatca cgcagccggc ggccgcagcg ccaatcgaat ccggggcgcc ggctgatgac
     6961 ttccacggtg taaccggcgt ctttcagctc ttgccccaag ctgccgatgt cgtcgagcag
     7021 tgcaaacacc ggccggaagt cccgctcgtg ctgaagggca aaggcccgcg cgagcacttc
     7081 gccgccgccg acgtgcagcg tgtgcagcac ctggcagatt actgggcggc catcgtccag
     7141 cgctggcggc cggcggactg gcattagcgc gcccagcatc gtggcagtcg tcatgggagc
     7201 cagctcgctc ccagcagtcg gcgcagccgc tcggccggcc aggtctggcg ggcagtgctg
     7261 taccaccgcc ccggctgcac agcacagccg attagcgaca gcagcccacg gggcgatcgc
     7321 agcgcgtggc ggatggcctg cttctgtggc atgaacaccg ccggctcgcc gatctccctg
     7381 ttgtcctcgc cgccgggcgc caaagcaggc gctctgtcct cactctgcag ataggcttcc
     7441 agatatccct gctgcatcgc cacggcgacg gggcgcgtgg tgcgccgcga ctcgaacgac
     7501 aggagcgatt gccaggcccg gtcgagcatc tcgcgccccc gctggcgaaa tcgcgcggcc
     7561 tcagcgccgg cggcgtagcg ggcggccatg agcagcgtgt ttcccttgcg cagctcctgg
     7621 gcggcccacg tttcggtcgg atattccagc ttctcgggct cgtcgagata gaaccgctcg
     7681 tgcgcggcca tccagcgggc gtagtgcaac agcgactggc gggcgtagtc tcgcaagaga
     7741 tcccactcgg ggacatctcc gatggcgtcg atcagccgca cgagcgcctg gagaaacacg
     7801 gcgtacgacc agcggcgttc tgcgcttgcc aaatcgcggg ctgcgatgtc gtcggccggg
     7861 tgaatgcagc ggcgtaccag ctcagcggcc ttagccaggt aatggctttc gccggtcaat
     7921 tgccaggcgt cgagcagcgc gtggatggaa ttcccggcgc cgcggcccgg accatgatag
     7981 ttggtttcgc aagtgcgact ggcatcgccg gtcggctgac tcgacaggat tcccagcagg
     8041 tggcggcagc cgtcgtccat cgccaggacc caatcggcca tgctgagtac ggctcgccga
     8101 gctcgctcgc agccagtgag atagtagtac agcgacaggc cacgggtgta gttgtgctcg
     8161 ttgcagggac ctgtgcccgg cgcggggatt ttcttaccct gcatcgccgc cgaaacgcag
     8221 cggtgcgagc ttttaccggc gtcgtgatag tgcgcggtgt gccagaacag gccgccgtta
     8281 taggccggct tgtcgcgcgt ggtgtggtag atgtcgatgt cgagcacgtg ccgcgccagc
     8341 ggatcggcca gggcccacca gcgggcgtcg ccgctcagca agtagtggat caatagactg
     8401 tgcaacaggt cgtattgatt gttgtagtgc gagatcaccg gtcgcggatc gtcgccaaag
     8461 gcctgttcgt ggtcggccca gacgtcgccg aaattgcgcc agccgtactc gtcgatccgc
     8521 tcccgcttgc cgagcaggct gtgggggccg tccgccgctt cccgcagcac gagctggtat
     8581 tcttcgcgga ctggcgcctg cggcgacggc gatagcggaa tcgctccgct cgcggccagc
     8641 cactccggat cggcgatggc gcgcggaggc cggtggaccc aactcagacc gtcgcacgga
     8701 gctgccgcat tgcggccgaa gtcgagccac accgttcgcg tgcaatgctc gccggcctgc
     8761 aattcatgca aatcgcccca gctgcccggc cagagttgag cgctgatacg attttcgtcc
     8821 accaccagac cgctgggaaa cttctgccag aactcttcga tcgcacacgc aacctggccg
     8881 gccgcgcctt ggattgccac ggcagggctg gcccgctcgc cgccgcattc gccatccgca
     8941 gtgcggaagc tgtacccgcg gaacctcagc ggcacgcggc cttcccgatt gacgtggttg
     9001 cggctgttcc actgcttgcc gccgctggat tcctgcagaa tctccaggcg tccagagacc
     9061 tcttgcgctt cgctgcccgg ctgctcggtc cacgctactc gcggctgatt gccaacgttt
     9121 gcttcaatct ctacggacca ctgcttcacg agcaccgaac ctggatcgcc caagtcccag
     9181 tagccgccgg ggtgccttgc ccgccgcgga ttctgcgccg tcagctcgat gcggacctga
     9241 gtcaggccgg cgaaaaagct gatgtgtccc ctcagccgca ggtcggtgcc gcggccgagc
     9301 acgcccgtga actccacttg ggcccgcagc ggaccgctct cgacaatcct tagcgattcg
     9361 atggttaccc gccgccgagc tccacgcgcg tcgatgcagc agatggcgac gcctttgttt
     9421 cccaaattct cgccgctgtc gctcgacgcg gacgacagga tccgcgcgtc gccgcgtcgc
     9481 aaccggcagc ggactttgcc ggtgtggacg acgatctcgg cgtcgctgct ctgagccacg
     9541 ctgacgggcc aaggcaattc gccagaatag tcctggtcat tcagcagcaa ttcccagggg
     9601 ccgtcgccgg c
//
LOCUS       DASRJB010000655         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1316951, whole genome shotgun sequence.
ACCESSION   DASRJB010000655 DASRJB010000000
VERSION     DASRJB010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316951"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFB80_17350"
     CDS             complement(<1..349)
                     /locus_tag="VFB80_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35601.1"
                     /translation="MRCFTAGLLIALLGASGFAKAADEPAGQFITLELVVADAPEGTP
                     ATPTAAAILELEKAGKLTSFSRYRLTALENQQSSLQFGELASLISGRQQGFRGAEGGV
                     PTFNQMSVGTMISA"
     gene            complement(587..1909)
                     /locus_tag="VFB80_17355"
     CDS             complement(587..1909)
                     /locus_tag="VFB80_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN35602.1"
                     /translation="MNARFSRRTLLTGVGVSMALPWLESLPVWGQEPPAKSDQPELPK
                     RFAALFMGNGISPNHWWAKGAGEAMEFSQSLQPLEPFKTKLNVVTGLFNKAATGVGIH
                     PGQTGNILSGASLQKGAVLRGGISVDQVLANHFADETAQPSLVLGCEQPITGYHETNF
                     SMAYSSHISWQDANSPVPMEVYPSLAFDSLFDNQGSQRTLSILDRVRDHAASIGRQVS
                     RADQAKLDEYLTSVREVEKRVERTRAMKDQADNNAADRGRPAIAMPRPDNGLPEDIRE
                     HMRLMCDLIALAFQTDKTRVATLLLCRDLSGLFYPFLDVRQAHHPTSHEDESDEYERV
                     TRYYVGQLAYLCQKLAAMPEGESTVLDNSCLMFVSNMWSGSKHDSSKLPVVLAGGLGG
                     RLATGRVLEYRDKGDRGRRLCGLYLSLLHKLGVPARQFGDASEPLADL"
     gene            complement(1985..2143)
                     /locus_tag="VFB80_17360"
     CDS             complement(1985..2143)
                     /locus_tag="VFB80_17360"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HZN35603.1"
                     /translation="MLSYALNRSLLLSDKKLLEQMQTKLAADDYAIGSLIETIVTSPQ
                     FVNRRGDL"
BASE COUNT          373 a          699 c          676 g          398 t
ORIGIN      
        1 cggcggagat catcgtgccg actgacatct gattgaaggt cgggacgccg ccttctgcgc
       61 ctcgaaaacc ctgctgccgt ccggatatga gcgacgctag ctcgccaaac tgaagcgacg
      121 actgctggtt ttccagggcc gtcagccggt agcgcgagaa gctggtcagc ttgcccgctt
      181 tctcgagctc caagatggcc gcggcagtgg gcgtcgccgg tgtgccttcg ggcgcgtcgg
      241 caacgaccag ctccagcgtg atgaactgcc ctgcgggctc atccgcggcc tttgcaaaac
      301 cactggcgcc gagcagcgcg atcaataagc cagccgtgaa acagcgcatg agaaaccccc
      361 ttcatgaaat gaagtatgcg gaatggcagc ggccagctcg ttccgcccat ccttcggacg
      421 ataaaccgcc gtgcggctgc gagggaccgc gacttcagac agaattcctg gatttcttga
      481 tgtaggcccc ttctgccgga aggggcctct cgggaagcgc agcgacctgg agattgtcca
      541 ggatttgacc ttgcagcatg cccaggtccc gcccggcagg cgggacctac aggtccgcca
      601 gcggctcgct ggcgtcgccg aactgcctgg cgggaacgcc cagcttgtgc agcagcgaca
      661 gatagagacc gcacagcctg cggccgcgat cgcccttgtc gcggtattcg aggacgcggc
      721 cggtggccag cctcccgccc agcccgcccg ccagcacgac cggcagtttc gatgagtcgt
      781 gcttgctgcc cgaccacatg ttggagacga acatcaggca agagttgtcg agtaccgtgc
      841 tctcgccctc gggcatcgca gcgagcttct ggcacagata agccagctgc ccgacgtagt
      901 accgcgtaac ccgctcgtac tcgtccgatt cgtcctcgtg cgacgtcgga tggtgcgctt
      961 gccgcacgtc gaggaacgga tagaagagtc ccgagaggtc gcggcagagc aggagcgtcg
     1021 ccacgcgcgt cttgtcggtc tggaaggcca gcgcgatcag gtcgcacatc agccgcatgt
     1081 gctcgcggat gtcctcgggc aggccgttgt cgggtcgcgg catcgcaatg gccggccggc
     1141 cgcggtcggc tgcgttgtta tccgcctggt ccttcatcgc ccgcgtccgc tcgacgcgtt
     1201 tttcgacctc gcgcacgctg gtcaaatact cgtcgagctt ggcctggtcg gcgcggctca
     1261 cttgccggcc gatcgaggcg gcgtggtcgc ggacgcggtc gaggatcgag agcgtccgct
     1321 ggctcccctg gttgtcgaac aggctatcga acgccagcga aggatagacc tccatcggca
     1381 cgggcgaatt cgcatcctgc caggaaatgt gcgagctgta ggccatcgag aaattcgtct
     1441 cgtgatagcc ggtgatcggc tgctcgcagc ccaaaacgag gctcggctgg gcggtctcgt
     1501 ccgcgaagtg attcgccagc acctgatcga cgctgatccc gccgcgcaac accgctccct
     1561 tttgcagcga cgccccggag agaatgttcc cggtctggcc aggatgaatg ccgacgccgg
     1621 tcgctgcctt gttgaacagg cccgtcacga cgttgagctt ggtcttgaac ggttccagcg
     1681 gctgcaggct ttggctgaac tccatcgcct cgcccgcgcc tttggcccac cagtgattcg
     1741 ggctgatgcc attgcccatg aacagcgccg caaaccgctt gggcagctcg ggctggtcgc
     1801 tcttggccgg cggttcctgg ccccacaccg gcagcgactc gagccacggc agcgccatcg
     1861 aaacgccgac gccagtcagc agcgtgcggc gtgagaatcg ggcgttcatt gttgacgttc
     1921 cttggaaagt gcgctgtagg aaacggcctc tgtgccgttc ccggaacgcc acggagtgcg
     1981 ttccctacag gtctccccgc cggttcacaa actgcgggct ggtcacaatc gtctcgatca
     2041 ggctaccgat agcataatcg tccgccgcca gcttcgtctg catttgctcc agcagcttct
     2101 tatccgacag cagcagactg cggtttaagg cataggagag cagctt
//
LOCUS       DASRJB010000656        12860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_757096, whole genome shotgun sequence.
ACCESSION   DASRJB010000656 DASRJB010000000
VERSION     DASRJB010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757096"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFB80_17365"
     CDS             <1..200
                     /locus_tag="VFB80_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35604.1"
                     /translation="AFDRSDDWLAWTHDGIYDGSPAVERYLAWRVGDELQTGAALGPQ
                     LRRAERLRAALQLNLPQESAP"
     gene            complement(314..622)
                     /locus_tag="VFB80_17370"
     CDS             complement(314..622)
                     /locus_tag="VFB80_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35605.1"
                     /translation="MNKVVISSLVAAVALLAVVSRVAMAEEVKHEGTISKIEGATVTV
                     KAADAQHEMTLSPTTKITLDGKPARASDLKVGFKVKTVCDKEGDRVTCKQFDATSGAN
                     "
     gene            1259..2977
                     /locus_tag="VFB80_17375"
     CDS             1259..2977
                     /locus_tag="VFB80_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35606.1"
                     /translation="MRFSKPVWRRVSARFVRWIGLSAVAASAGLLAFSGPEVYGQGRG
                     RDGGGGVRVQGGGNVRLPSVNGQVGGGATVRVPQGGGEIRVPAGGTVRVPEVRGPGAT
                     VREPQAGGEVRVPGAGGQVRVPGAGADVRVPSAGGEVRVPGGEGQVRVPGAGADVRVP
                     GAGADVRVPGAGGQVRVPGAGADVRVPGAGADVRVPGAGANVDVPGVPRTTLRPNLDA
                     NIRGGARLDGSASARVPDGVRIDSSVASQANLRFSPNWANITRERRVELDQFRTSLNN
                     TLRAQIRTDARVPGAAVDADARVGTNQWLALNPNRASVWSDWGTQARTGFRYGPSPFF
                     NYGWWNSRSLIGIGLGGYGYGVGPGYGGWWGYSPWIGYRPWWYWYSRPLWGSMIGWFP
                     GWGWSTPYYYDYGPGGNVVYTGNQVMVYGQPVGTATVYAQSAADLAAVDPASVGQTRP
                     EDWLPLGTFTLSTAKDEADPARVIQLAVNKEGLISGTSFNKQTGKTYTVQGRVDKDTQ
                     RMAFTIGNATDIVMETGLFNLTQDETPILAHMGPNRTATYLLARLPEPKHEAAEASAE
                     ATTTLP"
     gene            3151..3354
                     /locus_tag="VFB80_17380"
     CDS             3151..3354
                     /locus_tag="VFB80_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN35607.1"
                     /translation="MNWDTIKGDWKKFKGQIRERWGEFTDDELDKFQGRREQFEGMLQ
                     KKYGMAKDEAQRQLDEFEKSCSC"
     gene            complement(3451..3765)
                     /locus_tag="VFB80_17385"
     CDS             complement(3451..3765)
                     /locus_tag="VFB80_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35608.1"
                     /translation="MPRGDKSKYTDKQKRQAEHIEEGYKDRGIPEDEAERRAWATVNR
                     DSGGGKKPGGSGRGHKEDKSPMKKGGRLGGRAAARRPASERSASARKAARTRKDRASR
                     SR"
     gene            complement(3839..4363)
                     /locus_tag="VFB80_17390"
     CDS             complement(3839..4363)
                     /locus_tag="VFB80_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35609.1"
                     /translation="MAYAVYAIATSDAQAVRIGNRLRAAGFTPSDISVLTPDRGARDL
                     GHQVSTKAPEGATAGAGAGAILGGALGWLAGIGALAIPGVGPLIAAGPILGALTGAAV
                     GGAAGGLTGALAGLGIPEYEAQVYEGKLREGNTLLCVHVEDSEEASLARQILSEEKAE
                     SISTGTEASVPSRG"
     gene            complement(4423..4824)
                     /locus_tag="VFB80_17395"
     CDS             complement(4423..4824)
                     /locus_tag="VFB80_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN35610.1"
                     /translation="MRACAAALLVAAALVGCEKIDAPSGPVTNQTPPAPDNTAVNQRD
                     ADGATKTPIDQNENQADINTTAEIRKRILDTKDMSVNARNAKVITANGKVTLRGPVDS
                     DTERETIGKIAADVAGAGNVDNQLEVAAKNP"
     gene            complement(4872..5240)
                     /locus_tag="VFB80_17400"
     CDS             complement(4872..5240)
                     /locus_tag="VFB80_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35611.1"
                     /translation="MSTIFRNLLIGAVLSVSLLSLAPMPAQADDYWDGYWGWYDNTYR
                     PYYYRQYASPYVSPYRSGYMYGPSVYSYPSYPSSVYYGPRYQYGAPYGAYYGGPAVGY
                     SELGPYGAQVRVGPVRFGWR"
     gene            complement(5280..6263)
                     /locus_tag="VFB80_17405"
     CDS             complement(5280..6263)
                     /locus_tag="VFB80_17405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN35612.1"
                     /translation="MLKSDIAPSVGRWVVFGSMAAMGSVLALAQVTAPPEAPGAKGAK
                     DRAAPVAPREDQAQPRRPAERDRPAADRPAGADAQPARRPGLGIEFDAQAREGLSITT
                     IQPESLAAEAGLRAGDRFVTVDGRTFTTPRQLQAYLGGQFGRRIPVVIDRGGQQYNVY
                     LDMGQPGDDAAWLGVFLQESERQQPGAQITQVYPAGPAARAGLRPGDVIQQVNQKPVT
                     EPAELIGLIEEMKPGAKVELAVLRGQQQVTIPAVLGNRSHFVFYGGGQGNRSGEGQQF
                     DEDEFGNIPAYAMQLEHDRRMAEQHQRMEEELRKLTEEVRKLREALQPRAN"
     gene            complement(6292..6792)
                     /locus_tag="VFB80_17410"
     CDS             complement(6292..6792)
                     /locus_tag="VFB80_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZN35613.1"
                     /translation="MKMRTLQDLLVHEIKDLYSAEKQLVKALPKLAKAAGSHDLRAGL
                     ESHLTETEGHVNRLEEIMEQLEIPNRGPKCKAMEGLLEEGQEMLDEAMDDEVRDAAII
                     SAAQRVEHYEIAGYGCARTYAEQLGFEEAARMLQETLDEEKAADEKLTHIARQHINME
                     AAQGSR"
     gene            complement(6994..10170)
                     /gene="amrS"
                     /locus_tag="VFB80_17415"
     CDS             complement(6994..10170)
                     /gene="amrS"
                     /locus_tag="VFB80_17415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZN35614.1"
                     /translation="MSRAVTLPPAEPLLPDGSRLGGWWHDDTEPGRIVCDLCPRDCHL
                     RPGDRGFCFVRENRDGQMVLSTYGRSTGFCIDPIEKKPLNQFYPGTSVLSFGTAGCNL
                     GCKFCQNWSISKSREIEKLSESASPEAIAQAARRLGCDSVAFTYNDPVVWAEYAIDAA
                     RACREAGVKAVAVTAGYITAAARPTFYEHMDAANVDLKGFTEDFYRHLTLSHLEPVKE
                     TLRWLVHESPVWTEITNLIIPRENDSHDELRAMCDWVLEALGPNVPVHFTAFHPDFRM
                     LDTPRTPAETLLAAYDIAVKTGLNYVYVGNIAAERQQTTYCPNCKQPVIGRVGYTITR
                     YDMAGGKCRHCGAAVGGHYHERPGNWGSRRQPVRISEFEAAASGPKPASRPLKIEPAP
                     SPSSRASALSSEQEQAVLRSAVRVLAAATRGREVESTASDLDAAGELSVLGAFVSLKR
                     GGRLRSCCGFLGQNVPLASALAQAARRTATEDHRFPPIAPRELPHLDVEVWLLSNQQP
                     VKARGAAREEAVTIGRHGLQIVRGQLRGLLLPGVAVDHGLSAAEFLEHVCLKAELPPT
                     AWREDDVQLFTFEGHVIRGRVGDLVEPPAGAAVPISASELPALAEFCQGNLAALAAGA
                     TPNYFAQGLADGNVHGIVLSVLDAAGRELLQANRLSLRNTLPLQSSLFSLAENVAAAW
                     RRNSQTTDLRPSRVALTILHQPALHGTLAEPDWDGFDPRRHLLFVQERQRSAGVFDPA
                     QSAEHLAAAAARAAGVTSPAAAQLFALECTSSLARVQFSQVPRASHGAEVRPAGVAGR
                     FYPADPSELAQLVEQCLPVEPAVRQSWPALMVPHAGLIYSGRIAAQTLARVEIPKTVI
                     VIGPKHTPHGVEWAVAPHATWSIPGATLASDPELARQLCEAIPGWQLDAAAHAQEHAI
                     EVELPFLARLAPQAKVVGVALGTADFARCLQFAAGLAAVLRTLPAPPLLVISSDMNHF
                     ASDEENRRLDEIALAAMETLDPQRLYQTVTGRHISMCGLVPAVVVMQALRELNQLGRT
                     QRVAYATSADTSGDQSRVVGYAGMLLGH"
     gene            complement(10185..11969)
                     /locus_tag="VFB80_17420"
     CDS             complement(10185..11969)
                     /locus_tag="VFB80_17420"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN35615.1"
                     /translation="MDRRLLALLSVGLAFVLGCGSESSQPATAGNPASGGGQASGPEA
                     AETHRPIIVGHEPFDPQSFQPIEDPKALSRSFEMPSAQSPRDREGNPLRTADAAALRM
                     NPLRTLEPEAPSAAEPHPLVPRMAEPEIAPPATALPETAPPLELPESAPLAVEAAQPL
                     AGDLPSLPAAGGAALMPPAPPPETLPYEVVQVFYGTDRRAAEPLANDWPSRLVRFLPT
                     LCSGLLTVCMVLVASSRRKLGLWMLVFAGCGVSLGLGLQATSTTIAAIRQVGKSGRWY
                     TTERSAGNRVELGLCEVTIPRSHISGELEAPTVLRLEVREDAARHVVLRGTQRLASPK
                     FFELLQQRVEQSPRRELFIFVHGFNVSFEDAARRTAQMHHDLRFDGAPVFFSWPASDK
                     FVLTYAADESNVATSAPNLKQFLLEIVQRTQARSINLIAHSMGNRALGAALREIDLEL
                     RGESRLFNQVILAAPDLDAEDFRTNIAPALRRTAKGLTLYASSRDDALLASQLLHRGP
                     RAGDAGQGLVVIPGIDTIDVTAIDSSPWGHSYYGSSDPVLTDLKALLLQSTPAQDRTW
                     LSPAERDGQTYWIFQPARTAASPGHPPR"
     gene            complement(12140..12820)
                     /locus_tag="VFB80_17425"
     CDS             complement(12140..12820)
                     /locus_tag="VFB80_17425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN35616.1"
                     /translation="MSRRVVLTGAGRGLGLAMTEKLAELGHTVIGCTRQPAHALSLKK
                     RFGSPHDFDAVDVADDAAVGKWAKAVLGDGGPPDLLLNNAAVVNANAALWDVPQDEFS
                     RVIDVNVKGVYHVLRHFLPAMIKRQAGVIVNFSSGWGRSTSPEVAPYCATKYAIEGLT
                     LALAQELPAGMCAVPLNPGIINTDMLQSCFGDSAASYPTPEKWAKKAVPFLLALGPQD
                     NGKSLTAP"
BASE COUNT         2073 a         4206 c         4361 g         2220 t
ORIGIN      
        1 tggcgtttga ccgcagcgac gactggctgg cctggacgca cgacggcatt tacgacggct
       61 cgcccgcagt cgaacgctac ctggcgtggc gggtcggcga cgaactgcag acgggcgcgg
      121 cgctaggccc gcagctgcgc cgggcggaac gtcttcgcgc ggcgctgcaa ctgaatttgc
      181 cgcaggaaag cgcgccgtag agatttcgtc cggtgggcgc gcaaaaaaag cggcgtcgag
      241 tgcgaaccgg attctcgccc cgacgccgcc cttggaatgg cgcgccattg ccagttcgac
      301 tggcaattag cggctaatta gcgccggagg ttgcatcgaa ctgtttgcag gtcacgcgat
      361 cgccttcctt gtcgcagacg gtcttcacct tgaagccgac cttcagatcg ctggcccgag
      421 cgggcttgcc gtcgagcgtg atcttggtcg tcggggaaag cgtcatctcg tgctgggcat
      481 cggcggcctt cacggtcacg gtcgcgcctt cgatcttgct gatcgtgccc tcgtgcttga
      541 cctcttcggc catcgccaca cggctgacga ccgccagcag agccactgcc gccacgagcg
      601 agctgattac gaccttgttc atacgcttct ccttttccag tgtgagataa cccacggatc
      661 aggccggacc attgcccgac ggcgtcgcgc actgcaactt cggcaacgag caataagccc
      721 agccagcttg tcgcctcggc ccgtcagctt gcccattcct cacgtcactt cgcgacgctt
      781 atcgccgcat tgacgcgatc cggaatccgg aacaaggcaa gaacgagctt gtagcgattt
      841 gctgcctgag atcggagcaa gtgccgcgcc gccaagtccg gagtgaaagt gcggaagcgg
      901 acaaaactcg aaactctcgc tgcggcggtc taattcgtgg cattcatcat caacagatgc
      961 tcacgcttgc aaatttctgc tggcatggag cgggctttga atgatcgcac ggacacggtt
     1021 gtcgtcgtgg cttgccgttt cgtcgcacag cttgattcgt ggccaaccac aggtcgcggc
     1081 gacgtttggt gcccgattcc ccgcaatcgg tgggctgcgc tcacccaagt tgcctgaaga
     1141 tgcggcggca catcagttgc cccttattgt tgtgcgactc gcttggggga gctggcgggc
     1201 agcgcatttt ttgtttcagc gctcggagtg cgcgctttaa tcaacaagga gccagatcat
     1261 gagattctcg aaacctgttt ggcgccgcgt gtcggcgcgg ttcgtccgct ggattggtct
     1321 tagcgccgtg gccgcgtccg cggggctact ggcgttcagc ggaccagagg tctacgggca
     1381 aggacgcggt cgcgatggag gcggcggcgt tcgcgtccag ggcggcggca atgttcggct
     1441 gcccagcgtg aacggacaag tgggtggtgg cgcgacggtc cgcgtgccgc aaggcggcgg
     1501 cgagattcgc gtcccggccg gcggcaccgt gcgagtgccg gaagtccgcg ggccgggcgc
     1561 aacggttcgc gaaccgcagg ctggaggcga ggtgcgcgtg cccggggcgg gcggccaggt
     1621 ccgcgtgccc ggcgcgggtg cggacgtgcg agtaccaagc gcgggcggcg aagtgcgcgt
     1681 gcccggcggt gaaggccagg tccgcgtacc cggcgcagga gccgacgttc gagtacccgg
     1741 cgcgggcgct gacgttcgcg tgccaggcgc cgggggccag gtccgcgttc ccggcgcggg
     1801 agccgacgtt cgagtgcccg gcgcgggcgc tgacgttcgc gttcccggtg ctggcgccaa
     1861 tgttgacgtc cccggagttc ctcgcaccac cctgcgtccc aacctggacg cgaatatccg
     1921 cgggggcgct cgactggacg gttcggcttc ggcgcgagtt cccgacggcg tgagaatcga
     1981 ttcgtccgtc gcgtctcagg ccaacctgcg tttctcgccg aactgggcga acattacgcg
     2041 ggaacgccgc gtggagttgg accagttccg cacgagcctg aataacacgc tacgggcaca
     2101 gattcgcacc gacgcgcggg ttcccggcgc tgcggtggac gccgacgctc gcgtaggcac
     2161 gaaccagtgg ctggcgctca accccaatcg ggctagcgtc tggagcgact ggggcacaca
     2221 ggcccgcacc ggtttccgtt acggccccag cccgttcttc aactacggct ggtggaattc
     2281 gcgaagcctg atcggcatcg gcctcggcgg ctatggctac ggcgttggcc cgggctatgg
     2341 cggctggtgg ggctatagtc cctggatcgg ctaccgcccc tggtggtact ggtacagccg
     2401 tccgctgtgg ggctcgatga tcggctggtt ccccggctgg ggctggagta cgccctatta
     2461 ctatgactac ggcccaggcg gaaacgtcgt ttacacgggc aaccaggtca tggtgtatgg
     2521 acagcccgtg ggcacggcca cggtctacgc tcagtccgcg gccgacctgg cagcggtcga
     2581 tccggcatcc gtcggccaga cgcggccgga agattggctg ccgctgggca cgtttacgct
     2641 gtccaccgcg aaagacgaag ccgatccggc ccgcgtgatc cagctggcgg tcaacaagga
     2701 ggggctgatc agcgggacgt cgttcaacaa gcagaccggc aagacctaca cggtgcaggg
     2761 ccgcgtcgat aaggacaccc agcggatggc gttcacgatc ggcaatgcga ccgacatcgt
     2821 catggagacg ggtctgttta acctgacgca ggacgagacg ccgatcctgg cccacatggg
     2881 accgaatcgt acggcgacgt acctgttggc tcgcttgccg gagcccaagc atgaagctgc
     2941 tgaggcctct gcggaggcca cgaccacgct gccctaggtt agcgtgtgac cacagcggcc
     3001 gctcggcgct cagcgccggg cggctttttt cgtttagtga gccgctgctt gaccatagcg
     3061 gcgttggcac cggctttgct catttccatc actgggcagg gtagcgccat cgggcgcgct
     3121 gtccgacacg aacacaaaag gagaagagat atgaactggg acaccatcaa aggcgattgg
     3181 aagaagttca aaggccagat tcgcgagcgg tggggcgagt tcaccgacga cgaactcgac
     3241 aagttccaag gccgccgcga gcagttcgag ggcatgctgc agaagaagta cggcatggcc
     3301 aaggacgagg cgcagcggca gctcgatgaa ttcgagaaga gctgctcgtg ctgagcaatt
     3361 tcgctaaggg actctaaggc agcttgggga tggggcgcgg ctcggaagcc gcgccctttt
     3421 tgctgcgcgg tttgatttgc gagtgcgctc ctagcgcgag cggctggccc ggtccttccg
     3481 cgtgcgggcg gccttgcggg ccgaggcgga ccgctcggaa gccggccggc gagctgcggc
     3541 gcggccgccg agcctgccgc ctttcttcat gggggacttg tcttccttgt ggccccggcc
     3601 ggatcctcca ggcttcttgc cgccgccgct gtcgcggttg accgtcgccc aggcccggcg
     3661 ttcggcctca tcctccggga tgccgcggtc cttgtaacct tcctcgatgt gctcggcctg
     3721 gcgtttctgc ttgtcggtgt acttcgattt gtcgccgcgg ggcatgggat gactccataa
     3781 aaagaggagc cggcggaacg aatccccggc tccggaagtt ggttggattg cttgaatgct
     3841 acccgcggct gggcacgctg gcctcggtgc cggtcgagat gctctcggcc ttttcttcgc
     3901 tgaggatctg gcgggccagc gaggcctctt cggagtcctc gacgtgcacg cacagcagcg
     3961 tgttgccctc gcgcagcttg ccttcataca cctgcgcttc gtactcggga atgccgaggc
     4021 cggctagcgc gccggtcaag ccgccggcag ccccgcccac cgcggcgccc gtgagggcgc
     4081 cgagaatcgg cccggccgcg atcaggggac cgacccccgg aatcgccagc gcaccgatgc
     4141 cggcgagcca ccctagagcg ccgcccaaga tcgcacccgc gcccgcgccg gccgtggccc
     4201 cttcgggcgc tttcgtcgaa acctgatgac ccaggtcgcg tgcgccgcga tcgggcgtca
     4261 gcaccgagat gtcgctggga gtgaagccgg ccgcccggag tcgattgccg atccgcaccg
     4321 cctgggcgtc actcgtcgcg attgcatata ccgcataagc catgattttc tccttggttg
     4381 gctaggcaaa caggtaaacc gaagcagcag gtgtcacccc ggtcaggggt tcttcgctgc
     4441 aacctccagt tggttgtcga cgttccccgc tccggcgaca tccgcggcga ttttgccgat
     4501 cgtttcgcgt tccgtatcgg aatcgaccgg cccgcggagc gtcactttgc cgttggccgt
     4561 aatgaccttg gcattgcgag cgttcaccga catgtccttg gtgtccagaa ttcgcttccg
     4621 gatttcggcg gtcgtgttga tgtccgcctg gttctcgttc tggtcgatcg gagtcttcgt
     4681 agcgccatcg gcatcccgct ggttgacggc cgtattgtcg ggcgccggcg gggtttgatt
     4741 ggtcacggga ccgctcgggg cgtcgatctt ctcacagccc acgagggccg ccgcaactag
     4801 aagtgcggct gcacatgcac gcataggaga gtccttaagt tgagtttgtc gaaatcacca
     4861 ggtaccgact gctatcgcca gccaaagcgg acagggccga cgcgcacctg ggctccatag
     4921 gggcccaatt cgctatatcc aacagcgggg cccccgtaat aggccccata gggcgcgcca
     4981 tactgatagc gcggcccata gtaaaccgag ctgggatagc tgggatagct atacacgctc
     5041 ggcccataca tgtatccgga gcggtatggg ctcacgtatg ggctcgcgta ttggcggtag
     5101 taatagggcc gatacgtgtt gtcgtaccaa ccccaatagc cgtcccaata gtcgtccgcc
     5161 tgcgccggca taggggccag cgacaacagc gagacgctca gcacagcgcc gatcagtaga
     5221 ttacgaaaga tggtgctcat ggttagctcc caatagcttg ttatgtgcga agtaactcac
     5281 taattagcgc gaggctggag agcttcgcgc agcttgcgca cttcctcggt cagcttgcgc
     5341 agctcttctt ccatccgctg gtgctgctcg gccattcggc ggtcatgctc gagctgcatg
     5401 gcgtaggccg gaatgttgcc aaactcgtcc tcgtcgaact gctggccctc tccagaccgg
     5461 ttcccctgac cgcctccgta gaagacgaag tgggagcggt tgccgagcac cgcgggaatc
     5521 gtgacctgct gctggccgcg cagcaccgcg agctccacct tggcccccgg cttcatctct
     5581 tcgatcaggc cgatcagttc tgccggctcg gtgaccggct tctggttgac ctgctggatc
     5641 acatcccccg gtcgcaggcc ggcgcgggcc gccggcccgg cgggatagac ctgcgtgatc
     5701 tgcgcccctg gctgctgccg ttcgctttct tgcaggaaaa cgcctaacca ggccgcgtca
     5761 tcgcccggct ggcccatgtc cagatacacg ttgtattgct ggcccccacg gtcgatgacc
     5821 accgggattc ggcggccgaa ttggccgccc aggtaggcct gcaactgccg cggcgtcgta
     5881 aaggtccggc catcgacggt gacgaacctg tcgcccgccc gcaggcccgc ctcagcggcg
     5941 aggctttctg gctggatggt ggtgatgctc aggccttccc gagcctgggc atcgaactcg
     6001 attcctaagc cgggccgtct ggccggctgt gcatccgccc cagccgggcg gtcggcggcg
     6061 ggccggtccc gctccgctgg ccgccgcggt tgagcctggt cttcgcgagg cgcaaccggc
     6121 gcggcgcggt ccttcgcgcc cttcgcccca ggcgcttcgg gaggagccgt gacctgagcg
     6181 agcgccagga cgctgcccat cgcggccatg ctgccaaata caacccaacg gcccacgctg
     6241 ggcgcaatat cggacttcaa catatttggt tccccttgtt ggagtggagc gttagcggct
     6301 gccttgggcc gcttccatgt tgatatgctg cctggcaata tgtgtgagct tttcgtccgc
     6361 ggctttctct tcgtcgaggg tctcttgcaa cattcgtgcc gcctcctcga atcccaactg
     6421 ctccgcgtag gtgcgtgcac atccgtagcc ggcaatctca tagtgctcga cgcgctgggc
     6481 cgccgagatg atcgctgcat cgcgcacttc gtcatccatc gcctcatcca gcatctcctg
     6541 cccttcctca agcaaacctt ccatggcttt gcacttcggg ccgcggttgg gaatctccaa
     6601 ctgttccatg atttcttcga gccgattcac atgtccctcg gtctccgtca gatggctttc
     6661 caggccggcg cgaagatcgt ggctgccggc cgccttggcc agcttgggaa gtgccttgac
     6721 caattgcttc tcggcgctat acaggtcctt gatttcgtgg accagcagat cctgcagcgt
     6781 tcgcatcttc atcgccctgc tcccctgcga gagaatgttt gcagtccttc ttcccacaac
     6841 ccgcctggcg cgagtcaccg cctgaattcc gcggcaaacg atgtgccgcc cggtgtgggc
     6901 gcgccagggg cgaggcgggc agtcacagca gcgtcagggc ggcgagcgcg gcgacaggat
     6961 ggcacgaccc acagacggtg cggcagggcg ggatcaatgc cccagcagca tcccggcata
     7021 gccgacgacg cgggactgat cgccgctggt gtcggcgctg gtcgcatagg cgacgcgctg
     7081 ggtacggccg agctgattca gctcgcggag ggcctgcatg acgaccactg cagggacgag
     7141 gccgcacatg ctgatgtgcc ggccagtgac agtctgatag agccgctggg ggtcgagcgt
     7201 ttccatcgcc gccagggcga tttcatccag ccggcggttc tcttcgtcgc tggcaaaatg
     7261 gttcatgtcg ctggagatga ccaggagcgg cggcgccggc agcgtgcgga ggaccgccgc
     7321 cagcccggcg gcgaactgca ggcagcgagc aaaatcggcc gtgcccagcg cgacgccaac
     7381 gaccttggcc tggggcgcca aacgggccag aaacggcaat tcgacctcga tggcgtgctc
     7441 ctgggcgtgg gccgcggcat cgagctgcca gccggggatc gcttcgcaga gttgccgggc
     7501 cagttccgga tcgctggcca gggtggcgcc gggaatcgac caggtcgcgt gcggggcgac
     7561 tgcccactcc acgccgtgcg gcgtgtgctt ggggccgatc acaatcacgg tctttggtat
     7621 ctcgacgcgg gccagcgtct gggcggcgat ccgcccggaa tagatcagac cggcgtgcgg
     7681 gaccatgagg gcgggccagg actggcggac ggccggctcg actggcaggc actgctcgac
     7741 gagctgcgcc aattcggacg gatcggctgg ataaaaccgg cccgcgacgc cggccggccg
     7801 gacctctgcc ccatggctgg cccgcggaac ctgggagaac tgcacccgcg ccaggctgct
     7861 ggtgcattcc agcgcgaaga gctgtgccgc ggccggcgag gtgacccccg ccgcgcgggc
     7921 tgcagccgcc gccaagtgtt cggcagactg cgccgggtcg aacacgccgg ccgaccgctg
     7981 ccgctcctgc acgaagagca agtgccgccg cggatcgaag ccgtcccaat cgggctcagc
     8041 cagcgtgccg tgcagcgcgg gctggtgcag gatcgtgaga gccacgcgag agggccgcaa
     8101 gtccgtggtc tggctgttgc gccgccaggc agccgcgacg ttttcggcca ggctgaagag
     8161 cgacgactgg agcggcagcg tgttgcggag tgaaaggcgg ttggcttgca agagttcccg
     8221 gccggcggca tcgagcacgc tcagtacgat gccgtgcaca ttgccgtcgg ccagaccctg
     8281 ggcgaagtag ttcggcgtcg ccccggccgc cagcgccgcc agattgccct ggcaaaactc
     8341 ggccaaggcg ggcagctcgc tggcggaaat gggcaccgcc gcgcccgccg gcggctccac
     8401 gagatcgccg acgcggccgc gaatcacgtg cccctcgaac gtgaagagct gcacgtcgtc
     8461 ttcgcgccac gcggtcggcg gcagctcggc cttcaggcag acgtgctcca gaaactccgc
     8521 ggccgacagg ccgtgatcga cggcgacgcc cggcaggagc aatccgcgca attggccgcg
     8581 gacgatttgc agcccgtgcc ggccgatcgt aaccgcctcc tcgcgagcgg ccccgcgagc
     8641 cttcacgggc tgctggttcg agagcagcca gacctccacg tccaggtgtg gaagttcgcg
     8701 cggcgcgatc ggcggaaaac ggtggtcctc ggtcgccgtt cgccgcgccg cctgagccag
     8761 ggcggaggcc agcggcacgt tctgacccaa aaagccgcag cagctccgca ggcggccccc
     8821 gcgcttgagg ctcacgaacg cgccgagcac gctcagctcg cccgcggcgt ccaggtcgct
     8881 ggccgtcgac tccacctcgc ggccgcgcgt cgccgccgcc aggacgcgaa cggcgctgcg
     8941 cagcacggcc tgctcctgct cggatgagag cgcgctggct cgcgacgatg gcgatggagc
     9001 gggttcaatc ttcaacggtc ggctcgcagg tttcggtccg gaagcggccg cctcgaactc
     9061 gctgatccgc acgggctgcc ggcggcttcc ccaattgccg ggccgctcat gatagtgccc
     9121 gccgacggcc gcaccgcagt ggcggcactt tcccccggcc atatcgtacc gcgtaatcgt
     9181 gtagccgacg cggccaatca ccggctgctt gcaattcggg cagtaggtcg tctgctgccg
     9241 ctcggccgca atgttgccga catagacgta attcaggccc gttttcacgg cgatgtcgta
     9301 ggcggccagc agcgtctcgg ccggcgtccg cggagtgtcc agcatccgga agtccggatg
     9361 aaacgcggtg aagtgcactg ggacgttcgg gcccagagcc tccagcaccc aatcgcacat
     9421 cgcccgcaat tcatcgtgcg agtcgttctc ccgcgggata atcaggttcg taatctcggt
     9481 ccagaccggc gactcgtgca cgagccaccg cagcgtctcc ttcaccggct ccaggtgcga
     9541 gagcgtcagg tggcggtaaa agtcctcggt gaagcctttg agatcgacgt tggccgcgtc
     9601 catgtgttcg tagaacgtcg gccgggccgc ggcggtgatg tagccagccg tgacggcgac
     9661 cgccttcacg ccggcctcgc ggcaggcgcg ggccgcgtcg atcgcgtact cggcccacac
     9721 gaccggatcg ttgtaagtaa aggcgacgct atcgcagccc agccgcctgg ctgcctgggc
     9781 gatggcctcg ggcgaggcgc tttcgctgag cttctcgatc tcgcgcgact tgctgatcga
     9841 ccagttctgg cagaacttgc agcccaggtt gcagccggcg gtgccgaacg acagaacgct
     9901 ggtgccggga tagaactggt tgagcggctt cttctcgatc gggtcgatgc agaagccggt
     9961 gctgcggccg taggtcgaga ggaccatctg gccgtcgcgg ttctcccgca cgaagcagaa
    10021 gccgcggtcg cccggccgca ggtggcaatc gcgcgggcag aggtcgcaca cgatccggcc
    10081 cggctcggtg tcgtcgtgcc accatccgcc cagccggctg ccatcgggca gcagaggctc
    10141 agccggcggc agcgtgacgg cgcgggacat accgcaatta tacgctaccg cggcggatgg
    10201 cccgggctgg cggcggtgcg ggccggctga aagatccagt acgtctggcc gtcgcgctcg
    10261 gcgggcgaga gccacgtccg atcctgggcc ggcgtcgact gcagcagcag ggccttcagg
    10321 tcggtcagca cggggtcgct gctgccgtag tacgaatggc cccagggact gctgtcgatg
    10381 gccgtcacgt cgatcgtgtc gatgcccgga atgacgacga gcccctggcc cgcatcgccg
    10441 gcgcgcgggc cgcggtgcaa gagctgcgag gcgagcaggg cgtcgtcgcg cgacgaggca
    10501 tagagcgtca gccccttggc cgtccgccgc agggccgggg cgatgttcgt gcgaaaatcc
    10561 tcggcgtcga ggtcgggcgc ggcgaggatc acctgattga acagccgcga ctcgccgcgc
    10621 agctccaggt cgatctcgcg gagggcagcg ccgagggccc ggttgcccat gctgtgggcg
    10681 atcaggttga tcgaacgggc ctgcgtgcgc tgcacgattt ccagcaggaa ctgcttcaga
    10741 ttcggggcgc tggtggccac gttcgattcg tcggcggcat acgtgagcac gaacttgtcg
    10801 ctggccggcc agctgaaaaa gaccggcgct ccatcgaacc gcagatcgtg gtgcatctga
    10861 gcggtccggc gggcggcgtc ctcgaacgag acgttgaagc cgtgtacgaa gatgaacagc
    10921 tcccgccgcg gtgattgctc cacgcgctgc tgaagcagct cgaagaactt ggggctagcc
    10981 agccgctgcg tgccgcgaag cacgacgtgc cgggcggcgt cctcgcggac ctccagccgc
    11041 agcaccgtcg gcgcttccag ctcgccggaa atgtggctgc gcggaatcgt cacctcgcac
    11101 aggcccagct cgacgcgatt gccggccgag cgctcggtcg tgtaccagcg gcccgatttg
    11161 ccgacctgac ggatcgcggc gatcgtcgtg ctcgtcgcct gcaggcccag gcccaggctc
    11221 acgccgcagc cggcaaagac cagcatccac aggcccagct ttcggcggct ggaggcgacg
    11281 agcaccatgc agaccgtgag caggccgctg cacagcgtcg gcaggaaccg caccagccgc
    11341 gacggccagt cgttggccag cggttcggcc gcccggcggt cggtgccata aaaaacctgc
    11401 acgacttcat aaggcagagt ctccgggggc ggcgctggcg gcatcagcgc cgcgccgccg
    11461 gcagcaggga gggaaggcag atcgccggcc agaggctgtg cggcctccac ggcgagcggg
    11521 gccgattcgg gtagctccag cggcggcgct gtttcgggga gagcggttgc tggaggtgcg
    11581 atttccggct ccgccatccg tggcacgagc gggtgtggct cggccgccga cggcgcttcc
    11641 ggctcgagcg tccgcagcgg attcattcgg agtgcggctg catcggccgt acggagcggg
    11701 ttgccttcgc ggtcgcgcgg cgactgggcc gaaggcattt caaacgaccg ggacagcgcc
    11761 ttgggatcct cgatcggctg gaacgactgc ggatcgaagg gctcgtgccc cacgatgatc
    11821 ggccggtgtg tctccgccgc ctcgggcccg ctggcctggc cgccgccaga cgccggattg
    11881 cccgccgtgg cgggctgcga actctccgag ccgcagccca ggacgaaggc caatcccact
    11941 gacaataaag caaggagacg ccgatccatg gccatgctcc gcgaggacaa gcgtccgctg
    12001 cgcgcgagct cagcgcgcac gatccgcccg atagcggcgg gatagtagtc ctggcgctcg
    12061 cagcacgcca gagcaaagag atccgcagac tgccgctagc acgaccttca aagccgcgac
    12121 cagtggtcgc gcggctcggc tagggagcag tgagcgattt gccgttgtcc tgggggccga
    12181 gtgccagcag aaacggcacg gccttcttgg cccatttttc cggcgtgggg taggaggcgg
    12241 cgctgtcgcc aaagcagctc tggagcatgt cggtgttgat gattcccgga ttgagcggca
    12301 cggcacacat ccccgcgggc agctcctggg ccagcgcgag cgtcagccct tcgatggcgt
    12361 acttcgtcgc gcagtaggga gcgacctcgg gcgaggtcga gcggccccag ccggagctga
    12421 agttgacgat cacgcccgcc tggcgcttga tcatcgccgg cagaaagtgc cggaggacgt
    12481 gatagactcc cttgacgttc acgtcgatga cgcggctgaa ctcgtcctgc ggcacgtccc
    12541 acagggccgc gttggcgttg acgacggccg cattgttcag cagcagatcg ggcggtccgc
    12601 catcgcccag gactgccttg gcccatttac cgaccgctgc gtcgtcggcg acgtcgaccg
    12661 cgtcaaagtc gtgcggggag ccgaagcgct ttttcaggct cagggcgtgg gccggctgcc
    12721 gagtgcagcc gatgacggtg tgaccgagtt cggccagctt ctcggtcatc gccaggccca
    12781 gcccgcggcc agcgccggtc aagacaactc gacgactcat gtctttagcc gggaccaaac
    12841 ctggtcccgg agcggaggga
//
LOCUS       DASRJB010000657         7403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2732088, whole genome shotgun sequence.
ACCESSION   DASRJB010000657 DASRJB010000000
VERSION     DASRJB010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732088"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFB80_17430"
     CDS             complement(<1..322)
                     /locus_tag="VFB80_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35617.1"
                     /translation="MRRFLLPLLAVTFLAAAARGEEPAAASWETAAQKLQAATATVRI
                     WDAGDKHQTPPASVVVCSGVCVQKGKVVTSAFAGSDSRIRLTLAGGTQADAKLQLIDE
                     HSGLA"
     gene            complement(342..776)
                     /locus_tag="VFB80_17435"
     CDS             complement(342..776)
                     /locus_tag="VFB80_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35618.1"
                     /translation="MQFDPKLIHPEEPPLIAGGELDLPGDLAALGEQLSADAAHLAAC
                     YPAAPVQSAAAGRSSAWKMYAAFVLGPSLAVLLVAVIVWQSLPEQPPVDRTAAAVPAT
                     SPAIATPHAGDTVSLVELSGPELEALLDLLDRDPDRVTSISF"
     gene            complement(766..1326)
                     /locus_tag="VFB80_17440"
     CDS             complement(766..1326)
                     /locus_tag="VFB80_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN35619.1"
                     /translation="MRLDIDQTTQEHFARIHRAALVLCGNPWDADDLAQETFLVLAGQ
                     GGAFQGRSSIYTWLYGILLNLDRRERRRYGMRRRKLRVIWDSQPTTSATSPGANAAAE
                     VAEWKKSLWARVANLPDGQRQALVLRFSEGLRYEEIAEILECPLGTVKSRIFHGLGAL
                     RRLLDEEGEESRQLPAFPSEDLSHAV"
     gene            1604..3109
                     /gene="glgA"
                     /locus_tag="VFB80_17445"
     CDS             1604..3109
                     /gene="glgA"
                     /locus_tag="VFB80_17445"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZN35620.1"
                     /translation="MNIVFASSEVAPFSKTGGLADVCGALPIDLAQAGQNVAIITPAY
                     RQAKQAGLPIEPLGVKFDIPIGNKIVRGRLLRATLPNSEVPVFLVEQDDYFDRAELYR
                     LKGEDYKDNCERFVFFCRAALESIRLLHLQVDLFHCHDWQTALIPAYLHIEYLHTHGY
                     ENIASLLTIHNLAYQGIFWHWDMLLTGLDWKYFNWHQMEFYGKLNLLKTGLVFADWLT
                     TVSPTYALEIQSDPLGCGLDGILRQRSEQLTGIVNGVDYAAWNPAADQNLPERYDTAN
                     WQAGKAACKAALQRELSLPVSPRTPVVALIGRLADQKGWDLVIEVLRNWVHSTDAQWV
                     ILGTGEPFYHDTIGQLAREYPHRVAARLEFSDALAHRIEAGADIFLMPSRYEPCGLNQ
                     LYSLKYGTVPVVRATGGLVDTVSDTTPETLNAGKATGFAFHDLHAQALDAALRRAVDT
                     HWQNPDTWRQLVMTGMQQDWSWKASAQRYLEVYGHACRQHQDERERLKAHR"
     gene            3198..4235
                     /gene="galT"
                     /locus_tag="VFB80_17450"
     CDS             3198..4235
                     /gene="galT"
                     /locus_tag="VFB80_17450"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HZN35621.1"
                     /translation="MSEYRQDPLSRRWVIVGCDERAGRPNEFVEATTRQSGLPCPFCP
                     GNEAHTPAAVATYAANGKQGGWLVRVVPNKYPAVTLDQPTCPSCQPLASVPTSGHAQP
                     AAGNAAPGFGCHEVIIESPRHVASLSELTDVEAELVFRAYRDRIRDLKAEGRYKYVQI
                     FKNVGPAAGASLEHVHSQLVALPGVPEVLQQELAASADYFQQHQRPLLVDLIEQEQAA
                     MTRVVAQTANFVAFCPYASRFPYEVWIAPRRHQPRFEAVETGMLGELSRLAREIAGCI
                     ERAVGAAAYNAFLHTLPFDMPAYDHYHWHIEILPRLTKVAGFEWSTGCFINPYSPESA
                     ADHLRATLPVR"
     gene            4365..6542
                     /locus_tag="VFB80_17455"
     CDS             4365..6542
                     /locus_tag="VFB80_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZN35622.1"
                     /translation="MNSIRLCLVLHNHQPVGNFGGVFEQAYQDSYRLFLDVFEQYDLP
                     IALHTSGPLMEWLDERHPEYVDRLARLVAAGRIEVIGGPFYEPILTMIPPRDRVGQIA
                     NYSQWLESRLGGKVQGMWMPERVWEQSLTADLAAAGIRYTVLDDFHFKNAGLVEEQLH
                     GYYLTEDDGRLLSIFPGSEPLRYTIPFQQPQATIDYLRQIAARRAGAVVVFGDDGEKF
                     GVWPETKKHVYDNGWLRQFFDALVANREWLHLTTLAEATANVPPEGKIYLPDCSYREM
                     TEWALPVPQQIEYDHLVHEMERDDRWPRLKRFFQGGFWRNFRVKYPEANEMYARMMMA
                     SRRLEQVDQQGAGGDDLRYARQALYRGQCNCPYWHGAFGGIYLPHLRNAIYNQLIACD
                     NLLDKATGKTGGFVEATVDDYNFDARQEIRLANDKLIGLFAPAAGGMLYELDVRTICH
                     NLLATLARRPEAYHRKVLAGASNNGNCASIHDRVVFKQAGLEQRLQYDRFPRKSLLDH
                     FYEPAASLAAASRGEAHEQGDFVQGIYETKIRKSADRIQVQLTRLGRAGQHEVRITKG
                     ITLVAGSPKLEVAYLLENLPLSEPLHFAIELNLSGLPAGADDRYFHDGQGRRLGQLGK
                     QLDLSDAQNLNLVDEWLGIDVGLAFNRPTGLWAFPVETVSQSEGGFELVHQSVCVQPH
                     WLIRPDASGRWSVTFTLTASTALAEQRMRPAPQAQPELVGSAT"
     gene            7047..>7403
                     /locus_tag="VFB80_17460"
     CDS             7047..>7403
                     /locus_tag="VFB80_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35623.1"
                     /translation="MFARPHPFRRYWLLFTVLFGCGLLAVASIGNSQDPQRGADPQRS
                     PPAEAQRGADQPPAQSDRDKRLNAIEQTLQSLLKEVQALQKPDSDSGPPQTPAPSSTT
                     STGGGRRGQAPPPKLAL"
BASE COUNT         1341 a         2500 c         2322 g         1240 t
ORIGIN      
        1 aagcgaggcc ggagtgctcg tcgatcagtt gcagctttgc gtcggcctgt gttccgcccg
       61 ccagcgtgag gcgaattcgc gagtcggagc cggcgaaggc cgacgtgacg actttaccct
      121 tctgcacgca gacgcccgaa cagacgacga ccgaggccgg cggcgtctgg tgcttgtcgc
      181 cggcgtccca gattcgcacg gtcgcggttg cggcctgcaa cttctgggcg gcggtctccc
      241 acgacgctgc cgccggctct tcgccgcgcg cagcggcggc gaggaaagtg acggctagca
      301 gcggcagcag gaaacggcgc atcggactcg gacgacgaga atcaaaacga aatgctggtc
      361 acgcgatcgg ggtcgcgatc gagcaggtcg agcagggctt ccaactccgg accggacaac
      421 tctacgagcg aaacggtatc gccggcgtgc ggcgtagcaa tcgcgggcga cgtggccggc
      481 accgctgcag ccgtgcgatc gacgggcggc tgctccggga gtgactgcca aacgataacg
      541 gcaaccagca gcacggcgag cgacggaccg agcacgaagg cggcatacat cttccaagcc
      601 gagcttcgtc ccgccgcggc gctctgcacc ggcgcggccg gatagcaagc cgccaggtgc
      661 gccgcgtcgg cgctgagctg ctcgcccagc gcggccaggt cgccaggcag gtcgagctcg
      721 ccgccagcga ttagcggcgg ctcctcggga tgaatcaatt tgggatcaaa ctgcatggga
      781 gaggtcctcg ctcggaaacg caggcagttg ccggctctcc tcgccttctt cgtccagcaa
      841 tcgccgcaaa gcgcccaggc cgtggaagat tcgcgacttc acggtgccca gcggacattc
      901 cagaatctct gcgatctctt cgtaccgcag cccttcgctg aaccgcagga cgagcgcctg
      961 ccgctggccg tcgggcaggt tcgcgacgcg ggcccacagg ctcttcttcc actcggcgac
     1021 ttcggcagcc gcgtttgcgc cgggagacgt cgcactggtc gtcggctggc tgtcccagat
     1081 cacgcgcagc ttgcggcggc gcatgccgta gcggcggcgt tcgcggcggt cgaggttcag
     1141 cagaatgcca tacagccagg tgtagatcga gctgcggccc tgaaacgctc ccccttggcc
     1201 ggccaggacc aggaacgttt cctgcgccag atcgtcggca tcccagggat tgccgcacag
     1261 caccagcgct gcccgatgga tgcgggcaaa gtgctcctgc gtcgtctggt cgatgtcgag
     1321 gcgcacgcga gagtctcgga ctgaacttgg gtcgattgaa tcgattgtag cgggcggtcg
     1381 ttgcgaaagc gctatcggca gcaatgccac gctggcgcgc cctagttagt gttcgtgccg
     1441 ctggcggcgg ctgaagttca ggcaaaacgg gcggccgggg cggacttcgc cgaaaaggtg
     1501 tgccagctac ccgccgccgc gctggcccat gcccagtcca tacgctataa cctctcagtt
     1561 tgacgcatct tgcgacgaat tcgcgctgga cgggagcggc ctcttgaaca tcgtcttcgc
     1621 cagcagcgag gtagcgccgt tttccaaaac gggcgggctg gccgatgttt gcggggcgct
     1681 gccgatcgac ctggcccagg ctggccagaa tgtcgccatc attacccccg cctaccgtca
     1741 ggccaaacaa gcgggactgc cgatcgagcc cctgggcgtc aagttcgaca ttcccatcgg
     1801 caacaagatc gtccggggcc gattgctccg tgcgacgttg cccaactcgg aagttcccgt
     1861 gttcctggtc gagcaggatg actacttcga tcgcgcggaa ctgtaccgcc ttaaaggtga
     1921 agactacaag gataattgcg agcggttcgt gttcttctgc cgggccgcac tggagtctat
     1981 ccgcctgctc cacctccagg tcgatctgtt tcactgccac gactggcaga cggccctgat
     2041 ccccgcctac ctgcacatcg aatacctcca tacgcacggc tacgagaaca tcgcctcgct
     2101 gctgacgatt cacaacctcg cctaccaggg aatcttctgg cactgggaca tgctgctgac
     2161 ggggctcgac tggaagtact tcaactggca ccagatggag ttctacggca agctcaacct
     2221 gctgaagacc ggcctcgtct tcgccgactg gcttaccacg gtcagcccga cctacgctct
     2281 cgaaatccag agcgacccgc tcggctgcgg cctcgacggc atcttgcggc agcgaagcga
     2341 gcagctcacg ggcatcgtca atggcgttga ctacgccgct tggaaccccg cagcggatca
     2401 gaatctgccg gagcgatacg acacggccaa ctggcaggcc gggaaggccg cgtgcaaagc
     2461 ggcgctgcag cgcgagctga gcctgcccgt ttcgccgcgg actccagtcg tcgcgctcat
     2521 cggccgcctg gccgatcaaa agggctggga cctggtgatc gaagtgctgc gaaattgggt
     2581 ccactcgacg gacgcccagt gggtcatcct gggaaccggc gaacccttct atcacgacac
     2641 cattggccaa ctggcccgcg aatatccgca ccgcgtcgcc gcccgcctgg agttcagcga
     2701 cgcgctggcc caccggatcg aggcaggagc cgacattttc ctgatgccca gccgctacga
     2761 gccctgcggg ctgaaccagc tctacagcct gaagtacggt accgtgccgg tggtgcgggc
     2821 gacgggcggc ctggtcgata cggtgagcga caccacgccg gagacgctca acgcgggtaa
     2881 agccaccggg ttcgccttcc acgacctgca cgcccaggcc ctcgatgcgg cccttcgccg
     2941 ggccgtggac acccattggc aaaaccccga cacttggcga caactggtga tgacggggat
     3001 gcagcaggac tggtcctgga aggccagcgc ccagcggtat ctggaagtgt acggtcacgc
     3061 ctgccggcag catcaagacg aacgcgagcg gctgaaagca caccggtaga acaaggaccg
     3121 aagaaccaaa gaaccgaaga acccaattgc tgcatttggt tctttggttc ttcggttctt
     3181 cggagtttct ggccctgatg tcggaatacc gccaagatcc tctgtcgcgc cgctgggtga
     3241 tcgtcggctg cgacgagcgg gccgggcggc cgaacgaatt cgtcgaggcg acgacgcggc
     3301 aaagcggtct gccgtgtccc ttctgtccgg gcaacgaagc gcacacgccg gctgcggtcg
     3361 cgacctacgc ggccaacggc aagcagggcg gctggctcgt ccgcgtcgtg cccaacaagt
     3421 atccggcggt cacgctcgat cagccgacct gccccagctg tcagccgctg gcgagcgttc
     3481 ccacgagcgg ccacgcgcag cccgccgccg gaaatgcagc gccgggtttc ggctgccacg
     3541 aggtgatcat cgaatcgccg cggcacgtgg cgagcctgag cgagctgacc gacgtcgaag
     3601 cggagctggt cttccgcgcc tatcgcgacc ggattcgcga cctgaaggcg gaagggcgct
     3661 ataagtacgt gcagatcttc aagaacgtcg gtcctgccgc cggcgcgtcg ctggagcacg
     3721 tgcatagcca gctcgtcgcg ctgccgggag tgcccgaggt tctgcagcag gagctggctg
     3781 ccagcgcgga ctactttcag cagcaccagc ggccgctgct cgtggacttg atcgagcagg
     3841 aacaggccgc gatgacgcgc gtcgtcgccc agacggcgaa cttcgtcgcc ttttgtccct
     3901 acgccagccg cttcccctac gaagtctgga ttgcaccgcg caggcatcag ccccggtttg
     3961 aagccgtgga aactggcatg cttggcgaac tttcgcggct ggcgcgggag attgccggtt
     4021 gtatcgaacg tgccgtcggc gcggccgctt acaacgcgtt tctccacacc ttgccctttg
     4081 acatgccagc atacgaccac tatcactggc atatcgagat tcttccgcgc ctgacgaagg
     4141 tcgcgggctt cgagtggagc acgggctgct tcatcaatcc gtactcgccg gaatccgccg
     4201 ccgatcacct gcgtgccact ctgcccgttc gttagaaaca acggagtgcg ttttgcgctt
     4261 atgccgatat tgccggacag gcggcgtgcg ggagaaattg cgcgccgagt ctggcagtga
     4321 aacctacaat agccggcccg ccttgcccct ctgcccgccc caccatgaac tcgatccgtc
     4381 tctgcctggt actgcacaac catcagcccg tcggcaactt cggcggcgtc tttgagcagg
     4441 cttaccagga ttcctaccgg ctgttcctcg acgttttcga gcagtacgac ctgccgatcg
     4501 cgctgcacac cagcggcccg ctcatggagt ggctcgacga gcggcatccg gagtacgtcg
     4561 atcgcctggc ccgcctcgta gcggccggcc gcatcgaagt catcggcggc ccgttctatg
     4621 agccgatcct gacgatgatc ccgccccgcg accgcgtcgg gcagatcgcc aactactcgc
     4681 aatggctcga atcgcgcctc ggcggcaagg tgcagggcat gtggatgccc gagcgtgtct
     4741 gggaacagtc gctcacggcc gatctcgccg cggcgggcat ccgctacacc gtcctcgacg
     4801 acttccactt caaaaacgcg ggcctcgtcg aagaacagtt gcacggctat tacctgaccg
     4861 aggacgacgg ccggctgctc tcgatctttc ctggcagcga gcctttgcgg tacacgattc
     4921 cgttccagca gccgcaagcg acgatcgatt acctgcggca gatcgcggcg cggcgggccg
     4981 gagcagtggt cgtgttcggc gacgacggcg agaagttcgg cgtctggccg gagacgaaaa
     5041 agcacgtcta cgacaacggc tggctgcggc agttcttcga cgccctcgtg gccaaccgcg
     5101 agtggctgca cctgacgacg ctggccgaag cgaccgcgaa cgtgccgccg gagggcaaga
     5161 tttacctgcc tgactgctcg tatcgcgaga tgaccgagtg ggccctgccg gtgccccagc
     5221 aaatcgaata cgaccacctc gtccacgaga tggagcgcga cgaccgctgg ccgcggctga
     5281 agcggttctt ccagggcggc ttctggcgaa actttcgcgt caagtatccc gaggcgaacg
     5341 agatgtacgc ccggatgatg atggccagcc gccggctcga gcaggtggat cagcaaggcg
     5401 ccggcggcga cgacctgcgc tacgcccggc aggcgctcta ccgcgggcag tgcaactgcc
     5461 cctactggca cggcgcgttc ggcggcatct acctgccgca cttgcggaat gcgatctaca
     5521 accagctgat cgcctgcgac aacctgctcg ataaggcgac cggcaagacc ggcggcttcg
     5581 tcgaggccac ggtggacgac tacaacttcg acgcccggca ggaaatccgc ctggccaacg
     5641 acaagctgat cggtttgttc gcgcccgcgg ccggcggcat gctctacgag ctggatgtcc
     5701 gcacgatctg ccataacctg ctcgccacgc tggcccgccg gcccgaggcc tatcaccgca
     5761 aggtgctggc cggcgcgagc aacaacggca actgcgccag cattcatgac cgcgtcgtct
     5821 tcaagcaggc cggcctcgag cagcggctgc agtacgaccg cttcccccgc aagagcctgc
     5881 tcgatcactt ctacgagccc gccgccagcc tggctgccgc cagccgcggc gaagcccacg
     5941 agcagggcga cttcgtccag ggcatctacg aaaccaaaat ccgcaaaagc gccgaccgta
     6001 ttcaggtgca gctcacccgg ctgggccgcg ccgggcagca tgaggtgcga atcactaagg
     6061 gcattacgct ggtggccggc agcccgaagc tggaggtcgc ctacctgctg gaaaacctcc
     6121 cgctttccga gccgctgcac ttcgccatcg agctaaatct ctccggtttg ccggcggggg
     6181 ctgacgaccg ctacttccac gacggccagg gccgccgcct gggccaactc ggcaagcagc
     6241 tcgatctgtc cgacgcccag aacctcaacc tcgtcgatga atggctgggc attgacgtcg
     6301 gcctggcctt caaccggccg accggcctgt gggcgttccc ggtcgagacc gtcagccagt
     6361 ccgaaggcgg cttcgagctc gtccatcaat ccgtctgcgt ccagccccac tggctgatcc
     6421 gccccgacgc ttccggccgc tggtcggtca ccttcacgct gaccgccagc accgccctgg
     6481 ccgagcagcg gatgcggcct gccccacagg ctcagccgga gttggtcggc agcgcgacgt
     6541 aactgtagcc cccttctgcc ggaagggggc ctaggcgaag caagcgagca ggaacactgc
     6601 cgaagttgga cagcacagcg ctgcccaggt cccgcccggc aggcgggacc tactcagggt
     6661 aaggataatt cccattagcg cgccagccgt actctgggct gcgtgctccg cgacttgccg
     6721 caaacccttg tcgctgcgtg ctttgggatc gccaaggggc gttcctggag catgacgaac
     6781 ataagcattt gaggcgtgcg agcggaatta tccttacccc gccgctgagc actctgcgat
     6841 cgaatcctgg gacgaatcac ggactcagac tctgccaaaa tggcaaccgt ctgcgccaaa
     6901 cagaacttta ctgcaaatag cataccgatg ttcatacttt agcgtcgggc ttcccttttc
     6961 gcctcgactg acacccgcag gattgcaacc gggtttccgg cgctcacaat gggagcggtc
     7021 ttctcctctg gtccggaggt tctcccatgt tcgcccgccc tcatccattt cggcgctact
     7081 ggctgctctt cacggtgctc ttcggctgcg gcctgttggc cgtggcctcg atcggcaatt
     7141 cgcaagatcc gcagcgcggg gcagatccgc agcgcagccc gccggcagaa gcacaacgcg
     7201 gcgcggatca gccgccggcg caaagcgacc gcgacaagcg gctgaacgca atcgagcaga
     7261 cgctgcagtc acttctcaag gaggtgcagg cgctgcaaaa gcccgacagc gattccggcc
     7321 cgccgcaaac cccggcgccc agctcgacga cctcgaccgg aggcggccgc cgcggccagg
     7381 ccccgccgcc gaagctggcg ctc
//
LOCUS       DASRJB010000658         7289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1880382, whole genome shotgun sequence.
ACCESSION   DASRJB010000658 DASRJB010000000
VERSION     DASRJB010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880382"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFB80_17465"
     CDS             complement(<1..200)
                     /locus_tag="VFB80_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZN35624.1"
                     /translation="MSSPAKPSTLRQLRESGWRSKPVKREIHDNFLAALARGDELFPG
                     IVGFDSTVIPEINLALLAGHDQL"
     gene            complement(369..1235)
                     /locus_tag="VFB80_17470"
     CDS             complement(369..1235)
                     /locus_tag="VFB80_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35625.1"
                     /translation="MKPNRRAAFTLVELLVVIAIIGVLVALLLPAIQAAREAARASQC
                     KNSLKQLGLALHNYHDVMNRMPAGWIGSTPEGPPGWGWTTALFPYFEQKNLQESGIKF
                     NLPIDHAQNQAARETVLPLLLCPSDGHAKHFEIGADDAPGANIDGGTPLFKIARSNYV
                     GMFGTLEVEDAPSNGDGVFFHNSRVRFAEITDGLSNTLVIGERGVSYGNSVWAGVVDG
                     AAEAMVRIVGVADHTPNHPSLHFDDFSSYHPSGVHFLVGDGSVRRFNNSIDVAVYQGL
                     ATRGGGETAQAP"
     gene            complement(1380..3377)
                     /locus_tag="VFB80_17475"
     CDS             complement(1380..3377)
                     /locus_tag="VFB80_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35626.1"
                     /translation="MKNFVRVVLLTLRRRFTFLAAVVCSIGVALFWGGNLALIKPMIE
                     IVFTDKKPHALADAKVAEAQSKLATTRQELAAAQAALPKAPAEKRGELQAQQRRLAQR
                     LEVEKNAVRAAEFLRPLVHQFLPNDTFATLAIFVAAFVLATLLKDAMLVGNMLFVERL
                     TQLAMLDLRNSLFRQTLKMELAHFGDGHSSHITHRINSDVNCAFAGVNALCGRMILEP
                     LKMIACLIGAAIICWKLLLVSLLVAPLAAYILVRLTRSLRKANHKAMEDMGFLFTLLT
                     ETLTNIQTVKAFGMERHERRRYRQSGKSFYRRAMRITFFNAMGRASAEFMGMTIICLA
                     IVAGAYLVIHPNATILGFRVLEQPLSLGSLMAFYALLAGVSDPARKMSEVIGILYRGV
                     AASDRIYEILDAQPKIVDPPQPVTLTEAQPELIFDRVSFRYTTGQQVLHDVELRIPFG
                     QTVAIVGPNGCGKTTLANLLPRFYDPTAGAVRLAGIDLRELRLRELRKLTGLVAQQGL
                     LFDDTVLANIRYGSPWASEAEVVAAAEKAYAHRFVVDKLDKGYATAVGERGGRLSGGQ
                     RQRILLARAILRDPKFLILDEATSQIDLESEQLIHRVLEQFIRGRTTLLITHRMSSIA
                     LADRIVVMDAGRIIDTGTHDELNSRCELYARLCELGFQQAA"
     gene            complement(3558..5129)
                     /locus_tag="VFB80_17480"
     CDS             complement(3558..5129)
                     /locus_tag="VFB80_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN35627.1"
                     /translation="MTYLRIFCGLGIVAMLAAAFFAPPLTGDDKEVVIEKPKLDEKSP
                     SDKSKDDKAKEDKSKDDKAKDDKPEPGKTHTVARGPFKLEVSLPASIAAATTHEISID
                     PDSPLQLALREVVPHATRVTKGQTLLKFDTLKLDEEVRDLEASRALADLTLQQARREL
                     EILTKSAPLDAELAAQAKQHADEDLNRFEKLDRTFSEKEAAFGLKSANQYLEYTQEEL
                     KQLEKMYKADDLTEETEEIILKRARNDVEQMKFYVERANFMHDRELQVELPRFLVTHR
                     RAAAQAGLVLERTRLTQPLAIEKQKLEIQKQEFEQKKDADQLARLQADLKRLTVNAPA
                     AGVVYYGHWNNGKWSGTTEIGQKLRPGGQIQPYEVLLTLVEPDGVKVNAEVAEKELEH
                     VRAGIAGHFVAKAFPKQKLPVKVESLTAAPQSEGEFGATLTFEAAPPKSLAAGMTGDV
                     KIVAYFKADALTVPAKSVFRDEQNDEQRYVYRVDKEGKSARRDVTVGHESETTAEITG
                     GLAPGDKVLLEKPEE"
     gene            5280..6446
                     /locus_tag="VFB80_17485"
     CDS             5280..6446
                     /locus_tag="VFB80_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN35628.1"
                     /translation="MATDFLIVGGGIGGLVLAELLGRGGKRVVVLERSTAPPPWSRPE
                     ILWPATFELLCTLLPREQWEQRAVLPLEGMRIYDGRQWQWAISPDTLATAGVQPWSTN
                     PNQTREELMTLRSFELCRGIEVQEVLKEQGRIVGVRGRNSMTGTQQEWLAEWTVGDDG
                     ANSLVRSACGIELKTQIFPIDLFCFQAAWPAELRPRAGHVWPNVHESPPSVLATGLLP
                     VPGGQGVGLVPARTHADPGHRQAAFRKLVESEKSLLAVLGSRRFPEDFQLVRRPWGHA
                     PHYGCPGALLIGDAAHPVSPAGGQGANMSVADGRAVAELALAGERNLVAAYERRRRAA
                     NRRSLRFTRAAVFAFGLPEALLFNRLTGWLVSQVSSRPPIAARFIRTVATAFLE"
     gene            complement(6451..>7289)
                     /locus_tag="VFB80_17490"
     CDS             complement(6451..>7289)
                     /locus_tag="VFB80_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZN35629.1"
                     /translation="EPRLRIRPGESVVVESEDAFSGQVRTNDDRRDRTKKPFGNPQTG
                     PIWVEGAEPGDALAVKIHEIKPLIGQCATRTADLHLLATWLGTDTPHGTHVCPIKDGK
                     IYWSDEVTIHYAPMLGCIGTAPDMGVPTTFPAGPHGGNMDIIEVAPGNIVYLPVFVPG
                     GYLYLGDAHAAMGHGELSAAGLEMPAVTTISVDLLKGKKLAGPRIESPTEIMTVVSGC
                     PMERSQAEAFARLILWMEADYGWNRWRAYDLLTHVARISVGYFALGTVAVKVEKRYLS
                     GR"
BASE COUNT         1160 a         2360 c         2412 g         1357 t
ORIGIN      
        1 agctggtcgt ggccggccag caacgccagg ttgatttccg ggatgacggt gctgtcgaag
       61 ccgacgatgc cgggaaacag ctcgtcgccg cgggccaggg cggccagaaa attgtcgtgg
      121 atttcgcgtt tgacgggctt ggatcgccag ccgctttcgc ggagctggcg gagcgtggaa
      181 ggtttggctg gcgaagacat gcggcaactc ggcgtccgta cgaggcagtg gtgaggctga
      241 cccattatac ggcccgccct cggccggcga gcggccagcg tgagctggcc gatagcgtgg
      301 ggtctgtgct gcgggcgtct ctggcggggc agcgcaatcg ggaggctgac gcctcgctcg
      361 ccgagaagtt acggcgcctg cgctgtttcg ccgccgccgc gcgtcgccag gccctgatag
      421 acagcgacgt cgatcgaatt gttgaagcgc cgcacgctgc cgtcaccgac gaggaagtga
      481 acgccggagg gatggtagct cgagaaatcg tcgaagtgca gcgagggatg attgggcgtg
      541 tgatcggcga cgccgacgat gcggaccatc gcctccgctg cgccatccac cacgccggcc
      601 cacaccgaat tgccgtacga cacgccccgc tcgccaatga ccagcgtgtt cgagaggccg
      661 tcggtgatct cggcgaaccg cacgcggcta ttgtgaaaga acaccccgtc gccattcgac
      721 ggagcgtcct cgacctccag cgtgccgaac atgcccacgt agttgctgcg ggcgatcttg
      781 aacagcggcg tgccgccgtc gatgttcgcc cccggcgcgt cgtcggcgcc gatctcgaag
      841 tgcttcgcgt ggccgtccga cgggcaaagc aagagcggca gcaccgtctc gcgggccgcc
      901 tggttctgcg cgtggtcgat gggcagattg aacttgatcc cggattcctg gaggtttttc
      961 tgctcgaagt aggggaagag cgcggttgtc caaccccagc cgggcggccc ttccggcgtg
     1021 ctgccgatcc agccggccgg catccggttc atcacgtcgt gatagttgtg cagcgccaaa
     1081 ccgagttgct tgagcgagtt cttgcactgc gacgcgcgag ccgcctcgcg ggccgcctgg
     1141 atcgccggca acagcagcgc cacgagcacg ccgatgatcg cgatcaccac cagcaattcc
     1201 accagcgtga acgccgcgcg tcggttgggt ttcatggcgg gcagctccaa tcaacgtaag
     1261 cagaggcaat ccgtgactgg ctggtattgt gtcgcgaatg ctcgcaattg tcaagtgcat
     1321 ataaattgca ttaacggcct gtgggcatgg cgaagctggc ccgccggcag gcaacgacgc
     1381 taggcggcct gctggaaacc cagctcgcac agccgggcgt agagttcgca gcggctgttg
     1441 agctcgtcgt gggtgccggt gtcgatgatc cggccggcgt ccatgacgac gatccggtcg
     1501 gccagggcga tcgacgacat gcggtgagtg atgagcagcg tcgtgcggcc gcggatgaat
     1561 tgctccagca cgcggtggat gagctgctca ctctccaggt cgatctggct cgtcgcttcg
     1621 tccagaatca ggaacttggg atcgcgcagg atggcccgcg ccagcagaat ccgttgccgc
     1681 tggccgcccg agagccggcc gccgcgctcg ccgacggccg tggcatagcc cttgtcgagc
     1741 ttgtcgacga cgaagcggtg ggcgtaggcc ttttccgccg cggcgacgac ttccgcctcg
     1801 ctggcccacg gcgagccata gcggatgttg gccagcactg tgtcgtcgaa cagcagcccc
     1861 tgctgggcaa ccaggccggt cagcttccgc agctcccgca gccgcagctc gcgcaggtcg
     1921 attccagcta gccgcaccgc tccggcggtc gggtcgtaaa accgcggcag caaattggcg
     1981 agcgtcgttt taccgcagcc gttagggccg acgatagcca cagtctggcc gaagggaatc
     2041 cgcagctcga cgtcgtgcag cacctgctgc cccgtcgtgt accggaagga gacgcggtcg
     2101 aaaatcagct cgggttgtgc ttcggtcagc gtcacgggct gcggcggatc gacgatcttc
     2161 ggctgggcgt cgaggatttc gtagatgcga tcgctggcgg cgacgccgcg gtagaggatg
     2221 ccaatgacct ccgacatttt gcgggccgga tcgctcacgc ccgccaatag cgcatagaac
     2281 gccatcagcg atccgaggct caacggctgc tcgagaaccc ggaagccgag aatcgtggcg
     2341 ttgggatgaa tgacgaggta cgccccggcc acaatggcca ggcagatgat cgtcatgccc
     2401 atgaattcgg cgctggcccg ccccatcgcg ttgaagaacg tgatccgcat cgcccggcgg
     2461 tagaagctct tgccgctctg gcgatagcgc cgccgctcat gccgctccat gccaaaagcc
     2521 ttgaccgtct gaatgttggt gagcgtctcg gtcagcagcg taaagaggaa ccccatgtcc
     2581 tccatcgcct tgtggttggc tttgcgaagc gaacgtgtca gccgcaccag gatgtacgcc
     2641 gccagcggcg cgacgagcag cgagaccagc agcagcttcc agcagatgat ggcggcccca
     2701 atcaggcagg cgatcatctt cagcggttcc aggatcatcc gcccgcagag ggcgttgacg
     2761 cctgcgaacg cacagttcac gtcgctattg atgcgatgcg tgatgtggct ggagtggccg
     2821 tcgccaaagt gggccagctc catcttcagc gtctgtcgga agaggctgtt ccgcaggtcg
     2881 agcatcgcca gctgcgtcag ccgctcgacg aacagcatgt tgccgacgag catcgcatcc
     2941 ttgaggagcg tggccaggac gaacgccgcg acgaaaatcg ccagcgtggc gaaggtgtcg
     3001 ttgggcagga actggtggac cagcggccgc aggaattcgg ccgcccggac cgcgtttttt
     3061 tcgacctcca gccgctgggc gagccgccgc tgctgggcct gcaattcgcc gcgtttttcg
     3121 gctggcgctt tgggcagcgc ggcttgggcc gcggccaatt cctggcgcgt tgtggcgagt
     3181 ttgctctggg cctcggcgac tttggcgtcg gccagggcgt ggggcttctt atcggtaaag
     3241 acgatctcga tcatcggctt gatcagcgcc aggtttccgc cccagaacag ggcgacgccg
     3301 atcgagcaga cgaccgcagc caaaaacgta aaccgccgcc gcaatgtgag caggacgacg
     3361 cggacaaagt tcttcatgga cccaatggca tccggcggcg gggaaggcgt agcgccggcg
     3421 tgtcggtagc gataatggcg cacgatcccc cctggggtaa gagtggccgc cttgtagcac
     3481 aaagcggctc cccagaccaa gagcgacttc ttgccgagcc tcgctgaatt gcctggattt
     3541 cgcaggctgc cgggcggcta ctcttcgggc ttctccagca ggactttatc gccgggcgcg
     3601 aggccgccag tgatctcggc ggtcgtctcg ctctcgtggc cgacggtcac gtcgcggcgg
     3661 gcagatttgc cctccttatc gacgcgatag acatagcgct gctcgtcgtt ctgctcgtcg
     3721 cgaaagaccg atttcgccgg cacggtcagc gcgtcggcct tgaagtaggc gacgatcttc
     3781 acgtcgcccg tcatgcccgc ggccagcgac ttgggcggcg cggcctcgaa cgtgagcgtg
     3841 gccccgaatt ccccttcgct ctgcggcgcg gcggtgagcg actcgacttt caccggcagc
     3901 ttctgcttgg gaaacgcctt ggccacgaaa tgcccggcga ttcccgcgcg gacgtgctcc
     3961 agctcctttt cggccacttc cgcgttcacc ttcacgccgt ccggctcgac gagcgtgagc
     4021 agcacttcgt agggctgaat ctgcccgccg ggccgcagct tctggccgat ctcggtggtg
     4081 cccgaccact tgccgttatt ccagtggccg tagtagacga caccggcggc gggcgcattc
     4141 accgtcagcc gcttcaggtc ggcctgcagg cgggccaact ggtcagcgtc cttcttctgc
     4201 tcgaattcct gcttctggat ttcgagcttc tgcttctcga tggccagcgg ctgcgtcagc
     4261 ctggtccgct cgagcaccag gcccgcctgg gccgcggccc gcctgtgggt gacgaggaac
     4321 cgcggcagct cgacctgcaa ttcgcgatcg tgcatgaagt tggcccgctc gacgtaaaac
     4381 ttcatctgct cgacatcgtt gcgggcccgc ttgaggatga tctcttccgt ttcctccgtc
     4441 aggtcgtcgg ccttgtacat tttctcgagt tgcttcagct cttcctgggt gtattccagg
     4501 tactggttcg ccgacttgag gccgaacgcg gcctccttct cggaaaacgt ccggtcgagc
     4561 ttctcgaagc ggttgagatc ttcgtcggcg tgctgcttgg cctgggcggc cagctcggca
     4621 tccaagggcg cgctcttcgt gaggatttcc agctcgcggc gggcctgctg gagcgtgagg
     4681 tcggccagcg cccgcgaagc ctccaggtcg cgaacttcct cgtcgagctt gagcgtgtca
     4741 aacttgagca gtgtctggcc cttcgtaacc cgcgtggcgt gcggcacgac ttcgcgcagg
     4801 gcgagctgca gcggcgaatc gggatcgatc gagatctcgt gcgtggtggc cgccgcgatc
     4861 gacgccggca gcgagacctc cagcttgaat ggcccgcgag cgaccgtgtg cgtcttgccc
     4921 ggttccggct tgtcgtcctt cgccttgtcg tcctttgatt tgtcctcctt cgccttgtcg
     4981 tctttcgatt tgtcgctggg cgatttttcg tcgagcttcg gcttctcaat gacgacctct
     5041 ttgtcgtctc ccgtcagcgg cggcgcgaag aacgccgcag ccagcatcgc cacgattcca
     5101 agtccgcaga aaatgcgcag gtacgtcata catggccctt tcacagaagt tggcccgctt
     5161 gcttttgact cgttgcccga gtgggagttc cgcgggacag gctagcatgc ggcccggccg
     5221 gttgcacgcc gcgcggagag cggctgtggt cgccacagcg gcgtgttcta ataagggtga
     5281 tggccacgga cttcctcatc gtcgggggcg gaatcggcgg gctggtgctg gccgaattgc
     5341 tgggccgcgg cggcaagcga gtggtcgttc tggaacgcag cacggccccg ccgccgtgga
     5401 gccggccgga aatcctctgg cccgcgacgt tcgagctact ctgcacgctg ttgccgcggg
     5461 aacagtggga gcaacgggcc gtcctgccgc tggaagggat gcggatctat gacggacgcc
     5521 aatggcagtg ggccatttcg ccggacacgc tcgccacggc gggagtgcaa ccctggtcca
     5581 ccaatccgaa tcagactcgc gaagagttga tgacgctcag gtcgttcgag ctgtgtcggg
     5641 gcatcgaggt ccaggaggtg ctgaaggaac agggccgcat tgtcggcgtc cgcggacgga
     5701 attccatgac gggcacgcag caagagtggc tcgcggagtg gaccgtcggc gacgacggcg
     5761 cgaattcgct ggtccgctcg gcctgtggca tcgagttgaa gacgcagatc tttcccatcg
     5821 acttgttctg ctttcaggcg gcttggcccg cggagttgcg gccgcgcgcc gggcatgttt
     5881 ggcccaacgt ccacgaatcg ccgccgagcg ttcttgccac cggactgctg cctgtgcccg
     5941 gcggccaggg tgtgggtctc gtgcccgcgc ggacgcacgc cgaccctggt cacaggcaag
     6001 cggccttccg caagcttgtg gaaagcgaga agtccctgct ggccgtgctg ggcagtcggc
     6061 ggtttcccga ggacttccag ctcgtgcggc ggccgtgggg ccacgcgccg cattatggct
     6121 gcccaggagc gctactgatc ggcgatgccg cgcatcccgt cagtcccgcg ggcgggcaag
     6181 gggccaacat gtcggtagcc gacggccgcg ctgtggccga actggccctg gctggtgagc
     6241 gcaatctggt cgccgcctac gagcgccggc gccgcgccgc caaccgccgc agcctgcgct
     6301 tcacccgcgc cgccgtattt gccttcggcc tgcccgaggc cctgctcttc aaccgcctca
     6361 ccggctggct ggtcagccag gtcagcagcc gcccgccgat cgccgcccgc ttcatccgca
     6421 ccgtcgccac ggcgtttctc gaatagagca ctatcgtccg ctcagatacc tcttttcgac
     6481 cttcaccgcc accgtcccca gcgcgaagta gccgacgctg atccgggcga cgtgggtgag
     6541 caggtcgtaa gcccgccagc ggttccagcc gtagtcggct tccatccaga ggatcaatcg
     6601 ggcgaaggct tcggcttgcg aacgctccat cgggcagccg ctgacaacgg tcatgatctc
     6661 ggtgggggat tcgatccggg ggccagcgag cttcttccct ttcagcaaat cgaccgagat
     6721 cgtcgtgacg gcgggcatct ccaggccggc agccgagagc tcgccgtggc ccatcgcggc
     6781 gtgggcgtcg cccaggtaga gatagccgcc agggacgaag accggcaaat agacgatgtt
     6841 tcccggggcg acttcaatga tgtccatgtt gccgccgtgg gggccggcgg gaaatgtcgt
     6901 gggcacgccc atatcgggcg cggtgccgat gcagcccagc atcggggcgt agtgaatcgt
     6961 cacttcgtcc gaccagtaga tcttgccgtc cttgatcggg cagacgtgcg tgccgtgcgg
     7021 cgtgtcggtg cccagccacg tcgccagcag gtgcagatcg gccgttcgcg tcgcgcactg
     7081 gccgatcagc ggcttgatct cgtgaatctt caccgccagc gcatcgcccg gctccgcccc
     7141 ctcgacccag atcggccctg tctgcggatt gccgaagggc ttcttcgtcc ggtcccgccg
     7201 gtcgtcgttc gtccgcacct gtccgctgaa cgcatcctcg ctctccacga ccacgctctc
     7261 ccccggcctg atccgcagcc gcggctcat
//
LOCUS       DASRJB010000659         6092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_478667, whole genome shotgun sequence.
ACCESSION   DASRJB010000659 DASRJB010000000
VERSION     DASRJB010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478667"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2672)
                     /locus_tag="VFB80_17495"
     CDS             complement(<1..2672)
                     /locus_tag="VFB80_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35630.1"
                     /translation="MLALFARSQPRGLQHWILALAAAVSLAVPLASAQQTEDETLAGQ
                     LLQAGLNHANQGQWAASEEQLTRCLAVLQRVYPADRFPAGHLDQVVAHTLLAHAQEQQ
                     GKLVPALRGYAAAVKMLHGLYPAEQHPKGHPQLAQGLSTLAFFLSNHGMPQQSREHYE
                     KSLAMYAAAYRQEQSPDWLAAYASACGNYGGALWALGEADKALPHMELAVLLDEELYP
                     PAKYPQGHPRLAVGLENLAVIQEALNDFPAALATNRKVLAIRERAYPRDKFPSGHEKL
                     ARSHLSLAILLRKSGQLEQAAAERATALSTIERLHPAGHLITVQALNEQGLAAAEAGD
                     AAAAIVAFQKAIAVGERAFGDKADLDQQRAITVALANLGLVLKNEGRYTEALQTLLKH
                     QAAVERLYPAANYPQGHADLAHAQTSLANLRHSMGDFAAAREHHEQALAMLRRLFPPE
                     RFPRGHELLETELSSYGVFLNVTGDSAGALGLHKEALAMVDRLWPEPNHPKRLRKLGG
                     ALDNYGAALLAQRDFRGAERATRQSLAAYEQLYSKADFPNGHPDIALTLSNLGKCLSD
                     AGDFARARACSVRAIEMYKASYPPDKFPQGHPDWASALSNLGLKLHEQGERVEGKQYL
                     QQALAMRQQLFPDSLYPNGHPELAISLVNLATANINGANSTELSQAIAHLRQALAMKE
                     KLYAPELFPRGHESTVNSLINLADALLAAGETREAGVHAERAVDMQRGLAAGNLASLG
                     LASAVTKLGQVRSRLGDRDGKLALYREALEISQQCYPESAFPLGNRALVTALNNLGMT
                     LLERGEGEEGRALLAKATAMEERLAEAILLSASETQGVFYAEYHLGAPDHLLSVWPAD
                     ADLETLYGHVWRRRGAVAREMAARKLAVTVGDPQA"
     gene            complement(2873..4198)
                     /locus_tag="VFB80_17500"
     CDS             complement(2873..4198)
                     /locus_tag="VFB80_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35631.1"
                     /translation="MGIRSTILVALAALCGCSTYGRRVEQIRADFYSNQLAAASAAAT
                     EARQKSRAQADVLQLDQALIELAAGNAQESERLLREVRDRFDSLEGPAAGEAALSYLT
                     DDNRRAYDGEDYEKVLIRAFLALANLMHDGSDAEAYSLQLIDKQEQIIAAAAKPEQEN
                     PKANYQRVALAPYLRGVLREATHRDYDDAERSYTSVVSWQPQFQSGQADLARAAHGTH
                     SRPGHGVAIVFALTGRGPHKIEAIEVPSSAALLVAGEIVSAVGHQTVPPNVAPVKVPL
                     VVAQESRVHSVGVGVGNQPLGTTETITDVTQLAALQYRAIYPQVIARAVARRCLKKGA
                     LYGAKEATGVQKGSLTGLAYDLAGIAWEATESADLRCWSLLPDKIQVFRLELPAGEHD
                     LSLRPLGQANTPLASAATRRIRIADGRNTYVLASFPDGPLVGQVLVSQP"
     gene            complement(4289..4975)
                     /locus_tag="VFB80_17505"
     CDS             complement(4289..4975)
                     /locus_tag="VFB80_17505"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZN35632.1"
                     /translation="MLRRNFLRAATAAAGCAGLGLAGCRGTQYARVIKPGEQAMVGSH
                     AAGQETFTPLIDEAVGQLLARHDHSPYRQVSTGPEPLPPPKLRVCFVGVENCTAEEIG
                     DFKAQIYERIDTRLLQCGTVAPISRRFVDAALVQTRLRPEQLFVPENMRMFVGLLEQQ
                     GQPFDYLLFAKLTSGTTRENRDYQRDYDLTLELVDIRTGEQDKQTATLSKGYHHSWTS
                     RTLSQFWPWR"
     gene            complement(5051..>6092)
                     /locus_tag="VFB80_17510"
     CDS             complement(5051..>6092)
                     /locus_tag="VFB80_17510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35633.1"
                     /translation="HSEFEVVVIIDGREPETEREVAALLAPHDQLVTVARGSLHDCYN
                     AGRAVARGEALFFTESHVKAAPDCVQQMIARLAAGDADCVAVASGGIDEDRFAAQEQT
                     IYEQALPGRIAGGWNLCTVRGFAVKRDALDRAGGFLARYNHYSELLLGAALKHTGARL
                     GYAPAARVWHFNSGSLEHFARELNAFGRDEIRFRAENPASPLLEFLGPCAVWEQRQSL
                     ARGAALRRTWAALAGICRSLFRGRLKDAGREVEAVCRFAPCVLLGPMWLQLKAAAAVQ
                     WAILWLYLGCFSDRLYYRAFRAMWNGQIRRGRIDAVAELVSAAAVDGAASARATRAAS
                     CPIDAAGTASNC"
BASE COUNT          975 a         1978 c         2103 g         1036 t
ORIGIN      
        1 gcctgcggat cgcccactgt cacggccagc ttgcgcgcgg ccatttcccg cgcgaccgcg
       61 ccgcggcgac gccagacgtg accgtagagc gtttccaggt ccgcgtctgc gggccagacg
      121 gagagcaaat ggtcgggggc ccctaaatgg tactcggcat aaaacacgcc ctgcgtttcc
      181 gacgcggaca ggagaatcgc ctcggccagg cgctcctcca ttgccgtcgc tttcgccagg
      241 agcgcgcggc cttcttctcc ctcgccgcgt tcgagcagcg tcatgccgag gttgttgagg
      301 gcggtgacga gtgctcgatt ccccagcgga aacgcggact ccggataaca ctgctgcgag
      361 atctccagtg cttcgcgata gagggccagc ttgccgtcgc gatcgcccag gcggctgcgc
      421 acctgaccaa gcttggttac ggcgctggcc agtcccaggg aggcgagatt gccggcggcc
      481 agcccgcgct gcatgtcaac ggcccgctcc gcatggacgc cggcttcgcg agtttctccg
      541 gcggcgagca gcgcgtccgc aagattgatc agggagttga ccgtgctctc gtggccgcgc
      601 gggaacagct ccggcgcgta cagcttctcc ttcatcgcca gtgcctgccg cagatgagcg
      661 atggcctgcg aaagttcagt ggaatttgcc ccattgatgt ttgcagtggc gagattgacg
      721 agactgatgg ccagctccgg atgaccattg gggtagagcg aatcggggaa gagctgctgc
      781 cgcatggcca gggcttgctg caggtattgc ttcccttcca cgcgctcgcc ctgctcgtgg
      841 agcttcaacc ccagattgct cagggcagag gcccagtccg gatgtccttg gggaaacttg
      901 tccggcggat agcttgcttt gtacatttcg atcgcccgca ccgaacaagc ccgggcacgc
      961 gcaaaatctc cggcgtcact gaggcacttg cccagattgc tcaacgtcag cgcaatatcc
     1021 ggatgtccgt tgggaaagtc cgccttggag tagagctgct cgtacgcggc cagcgactgg
     1081 cgagtcgcgc gttcggcgcc gcgaaaatct cgttgggcca aaagcgccgc gccgtaattg
     1141 tcgagggctc cgccaagttt tctcaggcgc ttggggtgat tcggttccgg ccacagccgg
     1201 tcgaccatcg ccagcgcctc cttgtgcaat cccagcgccc cagccgagtc gcctgttacg
     1261 ttcaggaaga cgccatagct cgagagctcg gtctccagca gctcatgccc gcgcggaaac
     1321 cgctccggcg gaaagaggcg gcgcaacatg gccagggcct gctcgtgatg ctcgcgggcc
     1381 gcggcgaagt cgcccatcga atgccgcaga ttggccaaag acgtctgtgc atgggccagg
     1441 tcggcgtgac cctgcggata gtttgccgca ggatagagcc gctcgacggc ggcctggtgc
     1501 ttgagcagcg tttgcagcgc ctcggtgtac cggccttcgt tcttcaacac caatcccagg
     1561 ttggccagag ccacggtgat tgcgcgctgc tgatcgagat cggccttgtc gccgaaggcc
     1621 cgctcgccca cggcgatcgc cttttggaat gcgacgatcg cggcggccgc gtcgcccgcc
     1681 tcggcggcgg cgaggccctg ttcgttcagc gcctgcacgg tgatgaggtg gccagcgggg
     1741 tgcagccgct cgatcgtcga cagggcggtc gcccgttcgg cggcggcctg ctcgagctgg
     1801 ccgctcttgc gcagcaagat ggcgagggag agatgcgagc gggccagttt ctcgtgtccg
     1861 ctcgggaatt tgtcgcgcgg gtaggcccgc tcgcggatgg ccagcacttt gcggttggtg
     1921 gccagggcgg cgggaaaatc gttgagcgct tcctggatta cggccagatt ctcgagcccg
     1981 accgccagcc gcggatggcc ttgcggatat ttcgccggcg gatacagctc ttcgtcgagc
     2041 agcacggcca gttccatgtg cggcagcgcc ttgtcggctt cgccgagcgc ccacagcgct
     2101 ccgccgtagt tgccgcacgc cgaggcgtaa gccgcgagcc aatccggcga ttgttcctgc
     2161 cggtaggccg ccgcgtacat cgccaggctc ttctcgtaat gctcgcgcga ctgctgcggc
     2221 atgccgtggt tgctcaggaa aaaggccaac gtcgagagcc cctgggcgag ctgcggatgg
     2281 cctttcgggt gttgctcggc cggataaagc ccgtgcagca tcttcaccgc ggcggcatac
     2341 ccgcgcagtg ccggtaccag cttgccttgc tgctcctggg catgggcgag gagtgtgtgc
     2401 gcgacgacct ggtccaagtg ccccgccggg aatcggtccg ccggatagac tcgctgcagc
     2461 accgccaggc acctggtgag ctgctcttcg ctcgccgccc attggccttg attggcgtgg
     2521 ttcagccccg cctggagcaa ttggcccgcg agcgtttcgt cctccgtctg ctgggcagag
     2581 gccagcggca ctgcgaggct caccgcggca gccagcgcca gaatccagtg ctgaaggccg
     2641 cgaggctgcg aacgggcgaa caatgccaac atgactacct ccgcggcggc cggcagtttt
     2701 gggcgttgat cgtaccagac gggccggcgc gtctacctac cggtagcctg cggcgacgag
     2761 ttttcacccc ttgggacaga ctgagagtcc aacaaaacgg aggcagtgga gtccgcggcg
     2821 cgctgtaact tcaatcgcct atcgaggttg cggcggtcgc gaagagacca cgctacggct
     2881 ggctgaccag cacttgcccg acaagggggc cgtcgggaaa gctggccagg acgtaggtgt
     2941 tgcggccgtc ggcgatgcgg atgcggcgag tggctgccga ggcaagaggt gtgttggcct
     3001 ggccgagcgg gcggagagac aagtcgtgct cgccggcggg cagttcgagg cggaagacct
     3061 gaatcttgtc gggcaagagc gaccagcagc ggagatcggc cgactcagtc gcttcccagg
     3121 cgatgccggc caggtcgtaa gccaggccgg tgagcgagcc cttttgcacg ccggtggcct
     3181 ctttcgcccc gtagagcgcg ccctttttca aacagcggcg ggcgacggcg cgggcgatga
     3241 cttgcggata gatggcccgg tactgcaggg cggcgagctg cgtgacgtcg gtgatcgtct
     3301 cggtcgtgcc cagcggctga tttcccacgc cgacgcccac cgagtgcacg cggctctctt
     3361 gcgcgacgac gagcggcacc tttaccggag cgacgttcgg gggaactgtc tgatggccga
     3421 cggcgctgac gatttcaccc gccacgagca gtgccgccga gctgggcact tcgatcgcct
     3481 cgattttgtg cgggccgcgg ccggtgagcg cgaagacaat cgccacgccg tgacccggcc
     3541 gggagtgtgt gccgtgcgcc gcccgcgcca agtcggcctg gcccgactgg aactgcggct
     3601 gccagctcac gaccgatgta tacgaccgct cggcgtcgtc gtagtcgcga tgcgtcgcct
     3661 cgcgcagcac gccgcgcagg taaggcgcca gcgcgacccg ctggtagttg gccttcggat
     3721 tctcctgctc gggcttggca gcggcggcga tgatctgctc ctgcttgtcg atgagctgca
     3781 ggctgtaggc ctcggcgtcg ctgccgtcgt gcatcaggtt ggccagcgcc agaaacgccc
     3841 ggatcaggac tttctcgtag tcttcgccgt cgtaggcccg gcggttatcg tcggtcagat
     3901 acgagagcgc cgcctcgcca gcggccggtc cttcgagcga gtcgaaccgg tcgcggacct
     3961 cgcggagcag ccgctcggac tcttgcgcgt tccccgcggc cagctcgatc agcgcctgat
     4021 cgagctgcaa gacgtcagcc tgagcacggc tcttctgccg ggcctcggtc gccgcggcgc
     4081 tggcggcggc aagctggttg gaatagaagt cggcgcgaat ctgctcgacg cggcggccat
     4141 aggtcgagca gccacagagc gcggcgagcg ctacgaggat cgtcgatcga atgcccatcg
     4201 gcgagcggga ggcgtcagcc tcccgatgag ttgtgatcct ccggcgtgcg cggccatcgg
     4261 gaggctgacg cctcacgctc gccggttact atcgccacgg ccagaactgc gacagcgtgc
     4321 ggctggtcca ggagtggtga tagcccttgc tcagcgtggc cgtctgcttg tcctgctcgc
     4381 cggtgcgaat atcgacgagc tccagcgtca ggtcgtagtc gcgctggtag tcgcggttct
     4441 cgcgggtcgt gccggaggtc agcttcgcga acagcagata gtcgaagggc tgcccctgct
     4501 gctccagcag tccgacgaac atccgcatgt tctcgggaac gaagagctgc tcgggccgca
     4561 gccgcgtctg aacgagggcc gcatcgacga accgccggct gatcggcgca acggtgccgc
     4621 actggagcaa tcgcgtgtcg atccgctcgt agatctgggc cttgaagtcg ccgatctcct
     4681 cggcggtgca gttctcgacg ccgacgaagc agacgcggag cttgggcggc ggcagtggct
     4741 ccggaccggt cgagacctgg cggtaaggcg agtggtcgtg gcgggccagc agctgaccga
     4801 cggcctcgtc gatgaggggc gtgaaggtct cttgcccagc ggcgtggctg ccgaccatcg
     4861 cctgttcgcc cggcttaatg acgcgggcgt actgcgtgcc gcggcagccg gcgagtccca
     4921 acccggcgca gccggcggcg gcggtcgccg cgcggagaaa attacgacgc aacatgagag
     4981 gagttcctgg ataagcaaag cgacccgctt gggtctgtta tcggcagccg cgcggcacgg
     5041 gccgcagaca ctagcaattg ctcgccgttc cggcagcatc aattggacag ctagcagcgc
     5101 gagtggcgcg ggcgctggca gcgccgtcaa cggcggccgc ggaaaccagt tcggcgacgg
     5161 cgtcgatgcg gccgcggcga atctggccgt tccacatcgc gcggaaggcg cggtaataga
     5221 gccggtccga gaaacagccc aggtaaagcc agaggatggc ccactgcacg gctgctgctg
     5281 ctttcagctg gagccacatc gggccgagca gcacgcaggg agcgaagcgg cagacagctt
     5341 ccacttcgcg gcccgcatcc ttcaggcgtc cgcggaagag gctgcggcaa atgccagcca
     5401 gggcggccca agttcgccgc agagccgcgc cgcgagccag gctttggcgc tgctcccaga
     5461 ccgcgcatgg tccaaggaac tccagcagcg gcgacgcggg attttctgcc cggaagcgga
     5521 tttcgtcgcg gccgaaggcg ttcagctcgc gggcgaaatg ttcgagcgaa ccggagttga
     5581 agtgccagac acgcgcggcc ggcgcgtagc ccagccgcgc acccgtatgc ttgagcgccg
     5641 cgccgagcag caactcggaa tagtggttgt agcgggccag gaatccgccc gcgcggtcga
     5701 gcgcgtcccg cttcaccgcg aagccgcgga ccgtgcatag gttccagccg ccggcgatcc
     5761 gcccgggcag ggcctgttcg tagatggttt gctcctgagc ggcgaagcgg tcttcgtcga
     5821 tgccgccgct ggcgaccgcc acgcagtcgg catcgcccgc cgccagccgc gcgatcattt
     5881 gctgtacgca atccggcgcg gccttgacgt gcgattcggt gaagaagaga gcctccccgc
     5941 gtgcaacggc gcggccggcg ttgtagcaat cgtgcagcga gccgcgggcg accgtcacca
     6001 gctggtcatg cggagccaac agcgccgcga cctcgcgctc cgtttccggc tcgcggccgt
     6061 cgatgatcac gacgacttca aactcgctgt gg
//
LOCUS       DASRJB010000660         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_192903, whole genome shotgun sequence.
ACCESSION   DASRJB010000660 DASRJB010000000
VERSION     DASRJB010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192903"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFB80_17515"
     CDS             <1..570
                     /locus_tag="VFB80_17515"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZN35634.1"
                     /translation="ALEWAVARSTKNTERVVWTAGHSTRSLEEFLKLLQASGVQLLAD
                     VRRFPGSRRHPHFGQAPLAAALAKAGVEYRHIEALGGRRTERLPNSPNTAWRVESFNA
                     YADYMQAAPFAAALEELMAAATAAVTAIMCSEALPQRCHRRLIADALTVRGWRVRHLL
                     SPTRIEDHALTPFARVDGTTITYPQAQLF"
     gene            643..975
                     /locus_tag="VFB80_17520"
     CDS             643..975
                     /locus_tag="VFB80_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZN35635.1"
                     /translation="MTASAQSNDKLLRHIVMYKFKEGLPAAQLQEVIDAFAGLPKKID
                     TIVGYEAGTNVSPEGKSEGFTHVFVVTFKTAADRDAYITHPAHQAYVQVVKDRREKVI
                     VFDYWTTK"
     gene            complement(1178..5590)
                     /locus_tag="VFB80_17525"
     CDS             complement(1178..5590)
                     /locus_tag="VFB80_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN35636.1"
                     /translation="MKRLSLVAPLGLSLALACSAHSAEPLKLLFLGDTGHHQPAARFR
                     QLAPAMKERGIELTYTDKMDDIDAENLGKYAGLVIYANTTRISPEQEQALLDYVAGGK
                     GLIPLHCASYCFLNSPKYIELVGAQFQRHGTGTFRTILAEAEHPIMKGFGGFESWDET
                     YVHTKHNEKDRTVLEYRDERGNKEPWTWVRTHGKGRVFYTAWGHDQRTWGNAGFLNLV
                     ERGIRWAVGEDPGVVPAFAASVGQAASLPVQQAVIDPNYTPEIKAKRTDIKPFEYTPA
                     KVAYYPPGRRTSGDGEWNKMQNPLADVAESMKHMVTPVGFEVKLFVAEPQLEGKPIFM
                     TWDERGRLWVCETYDYPNELQPAGQGRDRIRICEDTDGDGRADKFTLFADKLSIPTAI
                     AFYRGGALVQAGGETLYLKDTTGDDQADLRKTLITGWGMRDTHGEVSNFRYGLDNWYW
                     AMQGYNQSSPVLTDGRQVQGFRQGFFRFKVEGDGDKVAVTELEFLRSTNNNTWGLGHS
                     EDGLVFGSTANGNPSEFLPIPNRYYEAVRGWSSTVLNGIADSNRFYPVTENVRQVDFH
                     GGFTAAAGHALYTARNYPKEYWNRTAFVTEPTGHLIATFVLRREGAGYRSRNGWNLVA
                     SDDEWTSPIQAEVGPDGNVWFIDWYNYIVQHNPTPAGFRTGRGAAYETELRDKKHGRI
                     YRLVYVGQAAGLPASTAGQTGMSAAQLVAALKSDVMYTRLQAQRLLVERGNKDVVPAL
                     VELVKDQKVDAIGLNTAAIHALGTLTGLNAVQGDALAAAISGLKHPSAGVRRNALRVL
                     PPTEQSLAAIVASGVLNDSDVQVRLAALLALADLPASDAASAAAIAALSDSAVQQDRH
                     LLDATTAAVAKNGPAALLAAVKSTNTALASTAAAGRLTIVADHVARTGLDNPATLLTE
                     MKNAQPDIASAILTGLARGWPRDKEIALSPELETTIRELFVKLPPGGKSQLVTLATRW
                     GSQGLQEQIAKLAAGFLADASNSELGDVARIAAATRFVEINRSNAEATKLLELITPRT
                     SPELARGIVEALGKGESALVGSALVEALPRLTPAVRPAALSALLGKSTWTLALLNAIE
                     QGKVQLADLSLDQKQALATHPERQLASRARRLLEAGGSLPNPDRQKVLDDLLPLTKQT
                     ADATAGKLVYKNQCAKCHTHSGEGTKIGPDLTGMAVHPKHELLVHLIDPSRNVEGNYR
                     VYAVLTSDGVVINGLLASETKTSIELIDSEAKKHVVLREDIQNLQASNKSLMPDGFEK
                     QVKPEELANLLEFLTQRGQYVPLPLDKVATVVTTKGMFYDENAAAERLVFADWGPKTF
                     EGVPFMLVDPKVNTVPNAIMLRGPEGRIPPTMPRSVSIPCAIPAKAIHLLSGISGWGY
                     PYGQQKTTSMIVRLHYADGQTEDHNLINGEHFADYIRRVDVPQSKFAFDLRGKQLRFL
                     SIIPQRADSLARIEFVKGDDRTAPVVMAVTVETR"
BASE COUNT          926 a         1752 c         1908 g         1069 t
ORIGIN      
        1 gcgctagaat gggccgtggc ccgctcgaca aaaaacacgg agcgcgtggt ttggaccgcc
       61 ggccattcca cgcggtcgct cgaggagttt ctgaaactgc tgcaagcgag cggggtccag
      121 ttgctggctg acgtgcggcg atttcccggc tcgcgcaggc atccgcactt cggccaggcg
      181 ccgctggccg ccgcgctggc caaggccgga gtcgaatacc ggcacattga ggcgctggga
      241 ggcaggcgca ccgagcggct gccgaattcg ccgaacacgg cgtggcgcgt cgagtcgttc
      301 aacgcctatg ccgactacat gcaagcggcc ccatttgctg ccgctctgga agaattaatg
      361 gcagccgcga cagcagccgt taccgcgatc atgtgctccg aggccctgcc gcaaaggtgc
      421 caccgccgct tgatcgccga cgccttgacc gtccgcggct ggcgtgtccg gcacctgctc
      481 tcgccgacgc ggatcgagga tcatgcactc acgccgtttg ctcgagtgga tgggactaca
      541 atcacctatc cccaagcaca gctcttttaa tggagatccg ccgtggctcg tctcgccatt
      601 cctttggttc tttggctctt cggttctttg atcttatttc ccatgaccgc ctcagctcaa
      661 agcaacgaca agctcctccg ccacatcgtg atgtacaagt tcaaggaggg cctgccggcg
      721 gcgcagctcc aggaagtgat cgacgccttc gccgggctgc ccaagaagat cgacacgatc
      781 gtcggctacg aggcggggac caacgtcagc cccgaaggca agtccgaggg cttcacgcac
      841 gtcttcgtcg tcaccttcaa gaccgccgcc gaccgcgacg cctacatcac gcacccggcc
      901 caccaggcct acgtgcaggt cgtcaaggat cggcgggaaa aggtgatcgt gttcgattat
      961 tggacaacga aatgaggcgg cctacccacg agagacaagc gccacaagaa acaggattgg
     1021 cgcatgcgag gatggtggcc agatttcagg cacatcgtat tcgcaaacat gcccaaattc
     1081 ctcgcgagag ctagaggcat acgggaatga cgagacgctc caatggcagc cgagagccgg
     1141 cggccatggt tttcgtggcc cttagtgctt tttgtggcta tctcgtctcc accgtgacgg
     1201 ccatcaccac gggcgcggtg cggtcgtcgc ctttgacgaa ctcgatccgg gcgagcgagt
     1261 ccgcccgctg cgggatgatg gagagaaagc gaagctgttt gcctcggagg tcgaaggcaa
     1321 acttcgactg cggcacgtcc acgcggcgga tgtagtcggc aaagtgctcg ccgttgatca
     1381 ggttgtggtc ctcggtctgc ccgtcggcgt agtgcagccg cacgatcatc gacgtcgtct
     1441 tctgctgccc gtagggatag ccccagccgg agataccgga gagcaggtga atcgccttgg
     1501 cggggatcgc gcaggggatc gagaccgagc ggggcatcgt cggcggaatc cggccttcgg
     1561 ggccgcggag catgatcgcg ttgggaaccg tgttgacctt gggatcgacg agcatgaagg
     1621 gcacgccctc aaaggtcttt ggtccccagt cggcaaagac caatcgctcg gccgccgcgt
     1681 tttcgtcata gaacatgccc ttggtggtga cgacggtcgc cactttgtcg agcggcagcg
     1741 gcacatactg gccgcgctgc gtgagaaact ccagcaggtt ggccagctcc tcgggtttca
     1801 cctgcttttc gaagccgtcg ggcatcagcg acttgttgct ggcctgcaaa ttctgaatat
     1861 cctcgcggag gacgacgtgc tttttggctt cgctgtcgat caactcgatg gaggttttcg
     1921 tctcggaggc caggaggccg ttgatgacga cgccgtcaga cgtcagcacg gcatagacgc
     1981 ggtagttgcc ctcgacgttt cggctgggat ctatcaggtg cacgagcagc tcgtgcttcg
     2041 gatgcaccgc catgccggtc aggtcgggac cgatcttcgt gccttcgccg gagtgcgtgt
     2101 ggcacttggc gcactgattc ttgtagacca gcttgcccgc cgtcgcgtcg gccgtttgct
     2161 tcgtcagcgg caggaggtcg tcgagcacct tttgccggtc gggattaggc aggctgccgc
     2221 ccgcttcgag caggcggcgg gccctgctgg ccagctgccg ctcggggtgc gtggccaggg
     2281 cctgcttctg gtcgagcgac aggtcggcga gctgcacctt gccttgttcg atggcgttga
     2341 gcagggccag cgtccaggtg ctcttgccga gcagcgcgct caaagcggcg gggcggaccg
     2401 ccggcgtgag cctgggcaga gcctcgacca gggccgaccc gaccagggcc gattcgccct
     2461 tgccgagggc ctcgacgatt ccgcgggcca gctcgggcga ggtgcggggc gtgatcagct
     2521 ccaacagctt cgtcgcctcg gcattgctgc ggttgatctc aacaaatcgc gtggcggcgg
     2581 cgatgcgggc cacgtcgccc agctcgctat tgctggcgtc ggccagaaag ccggcggcca
     2641 gcttggcgat ttgctcctgc aggccctggc tgccccagcg ggtggccagc gtcaccagct
     2701 gcgacttgcc gcccggcggc agcttgacga acagctcgcg gatcgtcgtt tccagctcgg
     2761 ggctgagcgc gatctctttg tcgcgcggcc agccgcgagc caggccggtc agaatggccg
     2821 aggcaatgtc gggctgagca ttcttcatct cagtgagcaa tgtcgccgga ttgtcgagcc
     2881 cggtgcgggc gacgtggtcg gcgacaatcg tcagacgtcc ggccgcggcg gtgctcgcca
     2941 gcgccgtgtt cgtcgatttc acagcggcca agagcgccgc cggaccattc ttggcgacgg
     3001 ccgccgtcgt cgcgtcgagc aagtggcggt cctgctggac ggctgaatcg gagagcgccg
     3061 cgatcgcggc ggccgacgcg gcgtcgctgg ccggaagatc ggccagcgcc agtagcgccg
     3121 ccaggcggac ctgcacgtcg gaatcgttca gcacgccgct ggcgacgatg gcagccagcg
     3181 actgctccgt gggcggcagc actctcagcg cattgcggcg gacgccagcc gagggatgtt
     3241 tcaatccgga aatggcggca gcgagcgcgt cgccttgaac ggcgttcaag ccggtgagcg
     3301 tgccaagtgc gtggatcgcg gcggtgttca ggccgatggc gtcgaccttt tgatctttga
     3361 ctagttccac gagcgccggg acgacgtcct tgttgccgcg ctcgacgagc agccgctggg
     3421 cctgcagccg cgtgtacatc acatcgcttt tgagtgcggc gaccaattgt gcggcagaca
     3481 ttcctgtctg ccctgcagtc gaggcgggca agccggcagc ctgccccacg tagaccagcc
     3541 ggtagatgcg gccgtgcttt ttgtcgcgga gttcggtttc atacgcggcc ccgcggccag
     3601 tgcggaagcc ggcgggcgtg gggttgtgct ggacgatgta gttgtaccaa tcgatgaacc
     3661 agacgttgcc gtcggggccg acctcggcct gaatcgggct ggtccattcg tcgtcgctgg
     3721 ccacgaggtt ccagccgttc cgcgagcgat agcccgcgcc ctcgcggcgg aggacgaagg
     3781 tcgcgatcaa gtggcccgtc ggctcggtga cgaacgccgt gcggttccag tattcctttg
     3841 ggtaattgcg ggccgtgtag agagcgtggc cggcagccgc ggtgaagccg ccgtgaaaat
     3901 cgacctgccg cacgttttcg gtaacaggat agaagcggtt actgtcggcg atgccgttca
     3961 aaacggtgct gctccagccg cggaccgctt cgtaatagcg gttggggatc ggcaagaatt
     4021 cgctggggtt gccgttggcg gtcgacccga acaccagccc atcttcgctg tggcccaggc
     4081 cccaggtgtt gttgttcgtc gagcggagga actccagctc ggtgaccgcg accttatcgc
     4141 cgtccccctc gaccttgaag cggaagaaac cctggcgaaa accttggacc tggcggccgt
     4201 cggtcaggac gggcgacgac tggttgtagc cctgcatagc ccagtaccag ttgtcgaggc
     4261 cgtagcgaaa attgctcacc tcgccgtgcg tgtcgcgcat gccccagccg gtgatcagcg
     4321 tcttgcggag gtcggcctgg tcgtcgccgg tggtgtcctt aaggtagagc gtctcgccgc
     4381 cggcctggac cagcgccccg ccgcggtaga aagcgatcgc ggtcggaatg ctgagcttgt
     4441 cggcgaagag tgtgaacttg tcggcccggc cgtctccgtc ggtgtcttcg cagatgcgga
     4501 tccggtcgcg gccctggccc gcgggctgca gctcgttggg atagtcgtac gtctcgcaga
     4561 cccacaggcg gccgcgctcg tcccaggtca tgaagatcgg cttgccttcg agctgcggct
     4621 cggccacgaa cagcttcacc tcgaagccga cgggcgtgac catgtgcttc atcgactcgg
     4681 ccacgtcggc gagcggattt tgcatcttgt tccactcgcc gtcgccgctg gtgcggcggc
     4741 cgggcgggta gtaggcgacc ttggcggggg tgtactcgaa cggcttgatg tcggtgcgtt
     4801 tcgctttgat ttcgggcgta tagttcggat cgatcacggc ttgttgcacg ggcaaactgg
     4861 cagcttgacc tacggaggcg gcgaacgccg gaacgacgcc gggatcttcg ccgacggccc
     4921 agcggatgcc gcgctcgacg aggttcagaa agccggcgtt gccccaggtc cgctggtcgt
     4981 gaccccaggc ggtgtagaag acgcggccct tgccatgcgt gcggacccaa gtccagggct
     5041 ctttattgcc gcgctcgtcg cggtattcca ggaccgtgcg gtccttctcg ttgtgcttgg
     5101 tgtggacgta ggtctcgtcc cagctctcga agccgccaaa gcccttcatg atcgggtgct
     5161 cggcttccgc caggatggtg cggaacgtgc ccgtgccgtg ccgctggaat tgcgccccaa
     5221 ccagctcgat gtacttgggc gagttcagga agcagtacga cgcgcagtgc agtggaatga
     5281 gccccttgcc gccggcgaca tagtcgagca gcgcctgctc ctgctcgggc gagatccgcg
     5341 tggtgttggc ataaatcacc aggccggcgt acttgccgag attctcagca tcgatgtcgt
     5401 ccatcttgtc cgtgtaggtc agctcgatgc cgcgctcctt catcgccggc gctagctgcc
     5461 ggaaccgcgc cgccggctga tggtggccgg tatcgccgag aaagagcagt ttcaagggct
     5521 cggcagagtg cgccgagcag gcgagagcca acgacaaacc gagcggagcg accagcgaga
     5581 gacgtttcat ggcggacctg cgatgtgctg aatcggtgag gaggggctac ttggcggctg
     5641 cggactttgc cgcaa
//
LOCUS       DASRJB010000661         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3015872, whole genome shotgun sequence.
ACCESSION   DASRJB010000661 DASRJB010000000
VERSION     DASRJB010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015872"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFB80_17530"
     CDS             complement(<1..802)
                     /locus_tag="VFB80_17530"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35637.1"
                     /translation="MSPNHCGSRRGAFTLVELLVVIAIIGVLVALLLPAVQAAREAAR
                     RMSCQNNLKQLGLGMQMHHDTYLQLPTGGDRASGVRYVIGWPARIAPFIEQKNLREAI
                     DAKTANAIFFVMPWRNVIAPHDGASPLYVNPIPVLACPSSHLGKKSPDAWNTSDPQIN
                     AVNQAALHYRANGGSATSELIQGIWNNSSPHNRYCTSGVIHPASKVRYADITDGTSNT
                     LLFGETSSAFGRTLVSRSWGGIQPWTWGYYNYNDAAPVDDRNGWLMIDH"
     gene            complement(1019..2086)
                     /locus_tag="VFB80_17535"
     CDS             complement(1019..2086)
                     /locus_tag="VFB80_17535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35638.1"
                     /translation="MTIPSRRDLLAAGLAAGAGLAWPHRARSAQAPKIKVGQIGVGHA
                     HATKLEVYRRSSDYEVVGVVEPDASLRERAGQQPAYRDVTWLTEEQLLNTPGLSAVLV
                     ETKVRDLLNTAERCIAAGMHIHLDKPAGDSLPQFRRLLAAAEKNKRLVQMGYMYRYSP
                     AVILLRQMLRDRWLGDVFEIHTVMSKVVGPAERKQLAEFRGGIMFELGCHVIDSVVAI
                     LGKPERIHAFPRHSAAADDGLMDNMLAVFEYPSATASVKSAALEVDGGARRHFVVCGS
                     LGTFHIQPLDNPAVRLTLDRPRGEYKQGVQEITLPKFTRYVADAADMAQVLRGEKEFG
                     FSYAHDLAVQESVLRASGVAT"
     gene            complement(2113..2547)
                     /locus_tag="VFB80_17540"
     CDS             complement(2113..2547)
                     /locus_tag="VFB80_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35639.1"
                     /translation="MNTRSILAAIGFFIAAVHALADEVEKGVPQAPPSAKAVAGSYYC
                     GDGLGYNVTLTLKDDGTYTGEWHGCLGKYGDAAGSWKLSDTRIVLTPTTETDAMTGHL
                     KTLDVLRYKEGWIFVRADRRDFYDKYGVSRVSCFLKMAEKQE"
     gene            complement(2779..>3111)
                     /locus_tag="VFB80_17545"
     CDS             complement(2779..>3111)
                     /locus_tag="VFB80_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35640.1"
                     /translation="SAGQRLHLAIATRRLRLLKAYRDYLDQFGGQRGAEGFGHLLAAA
                     DAGIVELKSATQVRRDYIESAVAMARELAELTRAAQRGPLSQLLDGPSDKDLRADENI
                     RKTPGGAH"
BASE COUNT          521 a         1015 c          982 g          593 t
ORIGIN      
        1 tgtggtcgat catcagccag ccgttgcggt cgtccacagg ggccgcgtcg ttgtagttgt
       61 aataacccca agtccaaggc tggatgccgc cccagctgcg cgagaccagc gtccggccga
      121 atgccgacga cgtctcgccg aagagcagcg tattggaggt gccgtccgtg atgtcggcgt
      181 agcggacctt gctcgcgggg tggatgacgc ccgatgtgca atagcggttg tgcgggctgc
      241 tgttgttcca gattccctgg atcagctcgc tggtggccga gccgccgttg gcccggtaat
      301 gcaaggccgc ctggttgacg gcgttgatct gggggtccga tgtgttccag gcgtcggggc
      361 tcttcttgcc caagtgcgac gacgggcacg ccagcaccgg gatcggattg acgtacagcg
      421 ggctcgcccc atcgtgaggc gcgatcacat tacgccacgg catgacgaaa aagatcgcat
      481 tggccgtctt ggcgtcgatc gcttcccgca ggttcttttg ctcaatgaac ggggcgatcc
      541 gagccggcca gccgatgaca taccgcacgc ccgacgcccg gtcgccgccg gtgggcaatt
      601 gcaggtaggt gtcgtggtgc atctgcatgc ccaggccgag ctgcttcagg ttgttctggc
      661 agctcatgcg gcgggcggct tcgcgggcgg cttgcacggc gggcaagagc agcgcgacca
      721 gcacgccgat gatggcgatc accacgagca gctcgaccag cgtgaatgct ccccggcggg
      781 aaccgcagtg attgggagac attgcgtctc ctcctttcaa aagcgccctg ccgtcagggc
      841 caacgggtgg gtcagtaagg ttatgtactc acaggcggca atgccgccag accagcgatc
      901 cccgttgcca gatcttggtc catctttatg caagcgtgcg agggtgtcaa cggtacctga
      961 attcgccaga attccgtctt ccggaccacc gaattctggc gaattcggct acatcccatt
     1021 acgttgccac tccactcgcc cgcagcaccg actcttgcac ggcgaggtcg tgggcgtagg
     1081 aaaagccgaa ttccttctcg ccgcgaagga cctgcgccat atcggcggcg tcggcgacgt
     1141 agcgggtgaa tttgggcagc gtaatctcct gcacgccctg cttgtactcg ccccgcgggc
     1201 ggtcgagcgt caggcggacc gcgggattgt ccagcggctg aatgtggaac gtgcccaagc
     1261 tgccgcagac aacaaagtgc cgccgcgcgc cgccgtcgac ttccagcgcc gcggacttca
     1321 cgctggccgt ggcggacgga tattcgaaca cggccagcat gttgtccatc aggccgtcat
     1381 cggccgccgc cgagtgccgc gggaaggcgt gaatccgctc gggcttgccc aggatcgcca
     1441 cgaccgagtc gatcacgtgg cagcccagct cgaacatgat cccgccgcga aactcagcca
     1501 gctgcttccg ctcggccggc ccgacgacct tgctcatcac cgtgtgaatc tcgaacacat
     1561 cgcccagcca gcggtcccgc agcatctgcc tcagcagaat caccgccggg ctgtagcggt
     1621 acatgtagcc catctgcacc agtcgcttgt tcttctccgc cgccgccagc agccggcgaa
     1681 actgcggcag cgagtcccct gccggcttat ccagatggat gtgcatgccg gcggcgatgc
     1741 accgctcggc ggtgttcaag agatcgcgga ccttcgtctc gaccagcacc gcggacagcc
     1801 cgggggtgtt gagcaactgc tcctcggtca gccaggtcac gtcgcgatag gcgggctgct
     1861 ggccggcccg ctcgcgcagg ctggcatccg gctcgacgac gccgacgacc tcgtagtcgc
     1921 tcgagcggcg atagacctcc agcttcgtgg cgtgggcatg cccgacgccg atctggccga
     1981 ctttgatctt cggcgcctgg gcggacctgg cgcggtgcgg ccaggccaag ccagctcccg
     2041 cggccaggcc ggcggccagt agatcgcggc ggctagggat cgtcatggca ggcctcgcgg
     2101 gtggcggcta tgtcattcct gcttctcagc catcttcaga aagcaagaca cgcgggaaac
     2161 tccgtacttg tcgtaaaagt ccctgcggtc cgcgcggacg aagatccagc cctccttgta
     2221 tctcagcacg tccagcgtct tcaaatgacc cgtcatcgcg tcggtttcgg tcgtcggcgt
     2281 gagcacgatt cgcgtgtcgg acagcttcca gctcccggct gcgtcgccgt atttgcccag
     2341 gcagccgtgc cactcgccgg tgtaggtgcc gtcgtccttg agcgtgagcg tgacgttgta
     2401 gcccaagccg tcgccgcagt agtagctccc cgcgactgcc tttgcgctgg gcggggcctg
     2461 cggcactccc ttttcgactt catccgccag agcgtgcacg gcggcgatga agaagccgat
     2521 agcggccaga atgctgcgag tgttcatgtc gggcgctcca atcgtctcac gttatctctc
     2581 cgccgccgaa gccaccagac tcccccgtcg gagcctcgat tcgctcgcca ttgagtactc
     2641 gctattgaat ataagcgaaa ctgtcaagcc gcaatcgttt ggaaatgctc tggtgcttaa
     2701 tcgagtcttc gcaagtgatt acacatcagc catttactac cagatttggg ctccgcgatc
     2761 gcatgagcct ttgaagggtt agtgagcacc tccgggagtt ttccttatat tctcgtccgc
     2821 tcgcaagtcc ttatcgctcg gtccatcgag caactgcgac agcgggccgc gctgagccgc
     2881 gcgggtcaac tcggccagct cccgggccat ggccacggcg ctctcgatgt agtcgcggcg
     2941 tacttgggtg gcgctcttca gctcgacgat gccggcgtcg gcggcggcca ggagatggcc
     3001 gaatccctcg gccccgcgct gtccgccgaa ttgatccaag tagtcgcggt aggccttaag
     3061 caaccggaga cggcgcgtgg caatcgccag gtgtagccgc tggcccgcgc t
//
LOCUS       DASRJB010000662         9649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2449689, whole genome shotgun sequence.
ACCESSION   DASRJB010000662 DASRJB010000000
VERSION     DASRJB010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449689"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2868
                     /locus_tag="VFB80_17550"
     CDS             <1..2868
                     /locus_tag="VFB80_17550"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HZN35641.1"
                     /translation="RPAQTSAAPAGGLESVAPSSPSPAQQARATDLFLRGALTPEQLA
                     QARASRAGFEVVIGKSNFLPAAFLEIGAATSRATCQIRTSGVDFRGRSGSWTGSAFLI
                     GPNLLLTNHHVLNSPAVARAAQAVFNFQLGSDLRPLTPKSFRLRPDRLFVTSPIQRGL
                     DYTFCWIDGEPNREFGRVVVDRRAFAIAEDEFANIISHPDGRMKEISIQQNEVQWQDD
                     IVLHYSSDTQPGSSGASVCNNNWQLVALHHASQNSNVPEFQLLNEGIKLSAIAADLER
                     RASAGNSDAPLARELVALFRGTDERLGFFGALGRRTESQGLEAVVDAYQGTEQDVDIG
                     FWNVEWLTNRYDEKAQAVARVMKEFNLDVWCLEESSPNAAEAVVAELRNLGLDFATLP
                     AEPASADGKQSCTLLWNRATLNCVQEEWGQPIEEWLRSRSTEFDDLGLEAVHGRIFDR
                     YPALFKLTTVQPVNGNPVAFHLVPVHLKAMEEGSLRRQMASKILAAAAARKIADGAGE
                     DWIFGGDFNAELATDDFDALVTGGMVPISATDEAGGAFSYIKGPRSLIDHIFLSPNLA
                     ERYGAQDFFIVAADRTLPDYVSDISDHRPVLFRMSFQGGGEAAPPAPPLEAAVSDSPA
                     IAELKRMLAAAAQEPAAEEALDLEAKRKKKPKSGAVRGGERRASDYDDRDGYDAAFLG
                     NGSHRVPVPELSSTLSSHAVVVAPGAAGSARYLLPYTHFSLVMHGQRKLPILTAVNID
                     GTQSLKIAREGDKWYLDPRIPTEAQIGNELYTNNDLDRGHMVRRLDPVWGRRAVATVA
                     NDDTFHYTNACPQHKDLNQKEWSELEDYILDNAGVHGLRVCVFTGPVLRAGDRPYRDL
                     ALIPEDFWKVAVIIREDTGRMSATGYVLTQKDMISGLEFAFGAFRTYQVSLRSIERMT
                     GLDFGRLKDADPKRRRGGLEAAGGPEDEPPTEVAGPGSLVF"
     gene            2906..4096
                     /locus_tag="VFB80_17555"
     CDS             2906..4096
                     /locus_tag="VFB80_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN35642.1"
                     /translation="MKGIEFAEVRDAITRAFNSDEFDMFLFERFDFDRAVNVADGPFR
                     VVVQNVLKDFMQKGLDAVLIAEVAAQRPLKTDVQEVYRKYAKALVGEARQEEIDTERL
                     QALERYGLATKVVLQKAGQAQLPSATTPGEGFERTVKKYLPFLNPGQWRRDMFRRECC
                     VCRVEVGGGQMGTGFLVGPDALLTNYHVLQPVLEGQSPADKVRFRFDYHVQPNGVESP
                     GMLVELHPAEWNIDHSRFTAGEKAGNPDQTLPTTDELDYALVRLSRSVGQEPVDGETA
                     NGPPRGWIQLPTAAPAIVPQMPILILQHPQTEPLKLAMDTEGVLMVNANETRVRYTTN
                     TEGGSSGSPCFDMKWNLVALHHYGDPAYQHPVTYNQGIPIDAIRKRLVKQHKENALGG
                     PPPF"
     gene            4143..5207
                     /locus_tag="VFB80_17560"
     CDS             4143..5207
                     /locus_tag="VFB80_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35643.1"
                     /translation="MTINQLIVRLAELICDAFEEEQFSMLLFENVEEKEFDYLSDAKG
                     DTYKARVFFTVRWANRTGQVNKVLWLAQKVAGLRAGRADLHELAAQIGAAAAPAAAAP
                     GANGLPNELSVGITLLEQQLPKPLDHARDLRDLLRKKDCRAIDLPGRFHLSDRDIERL
                     VTVLALEAAPTIRYIRWLAERLVVEPPFIAFMAAKAMTTAMLRLGGDELQRAISAARN
                     AEDLLDGLVDADDATTTSFGLAARKEQLASALMLAEMRTKQGKFLLPASDLEAFLNEL
                     ITLFNRDTFDLMCRNGLQTASKFLGAHPHDPMELIAIQVFLTCRGKWERELIQAAQQA
                     QPASPVFQNLRQKFAAAAAG"
     gene            5216..6679
                     /locus_tag="VFB80_17565"
     CDS             5216..6679
                     /locus_tag="VFB80_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN35644.1"
                     /translation="MLTSPQLRKLHKALLEAFSLDDFESLLQCRIGARLELLATPGSM
                     QSQVSEVLKVAEQAGWTHELIRAAADARPNDPCFAALSEGLGLGAEIRVGGESATPNI
                     TECKTGAKLPEGFAAEPWREELPAMEARVCRVEVGGGIVGTGFLVGPDAVLTCYYVVE
                     HVIQQEVSPTAVRCRFDFRQLPDGRTEGLSVRLHSDWLIDREGQEPGELDFALLRLER
                     PLGAEPLNPAAPAEVKRGWVHLPADLPPVPVGAPLMVLSYALGGPLQLALETNGFIGF
                     VENDRLTYRMSTQPGAGGAPCFTSDWKLVALHEKRASADSDNPERFGISAAALRRKIE
                     KDGHSAALGHASSSESDTGSVLATAKPPKPKRVRNVDDPQKDRWGGKSKRSGRALRVE
                     DLLAANSAFTFNAVVASTDGSPLVGPVIFHLHDSYPKSVLRIHKIDEGNRAVLEEVSS
                     YGVYTIGAQVKDAQGKWIGLEYCLSEVPDLPARFMNR"
     gene            6691..8013
                     /locus_tag="VFB80_17570"
     CDS             6691..8013
                     /locus_tag="VFB80_17570"
                     /inference="COORDINATES: protein motif:HMM:NF039430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector-associated domain EAD1-containing
                     protein"
                     /protein_id="HZN35645.1"
                     /translation="MPPLTGAQFEELAAALAGADGAAAGAFDKDTLEQLVQFKLGRRL
                     FDFVAEDKPLKTIVFQLIRTAEMEGWTAALIQAVSITRPGHAQVQQFVAAHFPQAAAP
                     LLPAEQVERVKRGLAALATIVERLRDPEIRLIVGRFRADFEITREKVAALAGYKALHD
                     MLHQVEMRYLSSMADAAQLWRQSEAQVQALDRYAGQLLGDCERARQDARVLRNFRQEE
                     DWIDMLVQAARLVQQAVSGGEEAPLASGLNLLRGVLVHNPPRINHVLCEAAGDLRLKQ
                     LIEAMQQIEAQLGRTQPAAAPDDEFAAGLQELQLLQPRLGGLVAEHFEWQWLDREIAL
                     AATQPGVTPQARFFRWPDFQRRLLALCGLSPQKEWSQQLCANLTALTEAGSAGDSIQF
                     ARRFNKFRTEASVRFFNVDAELRDLSSQLAQVAQPLDQLLRIVTHGNG"
     gene            8000..9613
                     /locus_tag="VFB80_17575"
     CDS             8000..9613
                     /locus_tag="VFB80_17575"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35646.1"
                     /translation="MATAELPVESPPAEPLTSLRAIRAAHTELLQQVPADRIDAPQIA
                     LIADFLNRSAAAGAFLDLPEDRAAAQGLLDYWKSSLYSQRRQAAFGEGAVRLPAATLA
                     DFSPAAVEEIVKAAEARLVGDDKVQAARNLLLKLIQLPAERGTPQTDEVERSKLLERE
                     PRAAEQVLADLEAAGVLERFGDPPRVRLRADALLRRWPRLAAWLEKRLLFREAALFWD
                     RHNRDRGALFTRELLAQSHAYHDKNALEAEFVKEVNAEADRQEDRQEALHRQKVRTWR
                     RVAFAMGVMVLLDFVCLGYVAVTFSQRQQLAIEQKELAEAKLRQGRMMNMVGLLRALG
                     NIAAAESPAERQLAIKGWEVLEARGADDPEIAAFLKSFAGQIRTIATDRQRHANSAVQ
                     SMQYKELQGEVLELATAFRDDVVKSRDDASIQRLSELREDYYSIIRFLVEKIVAAAQS
                     GDSASSVQPYLREFWQLYWGEMGLVEGPDVKTGMVQFGNRLWIWSEHAAGRNEPFKDL
                     DGPAQRLFESLEKELKLPIEPSTPPAASL"
BASE COUNT         1764 a         3088 c         3158 g         1639 t
ORIGIN      
        1 cgtcccgctc aaacctccgc cgcgccggcc ggcggcctgg agtcggttgc tccttcgtcc
       61 ccttcaccgg cccagcaggc gcgggccacc gacctgttcc tgcgcggcgc gctaacgccc
      121 gagcagctcg cccaggcccg ggccagccgt gccggcttcg aggtggtcat cggcaagtcg
      181 aatttccttc cggccgcttt cctggaaatc ggcgctgcaa ccagccgggc gacctgccag
      241 atccgcacgt ccggcgttga ttttcggggg cgctcgggct cctggacggg ctcggccttt
      301 ctgatcggcc cgaacctgct gctcaccaac catcacgtgc tcaacagccc ggccgtggcc
      361 cgggcggcgc aagcggtatt caactttcag ctcgggtcgg acctccgccc gctgacgccc
      421 aaatcgttcc gcctgcggcc ggatcggctg tttgtcacca gcccgataca gcgcggcctg
      481 gactacacgt tttgctggat cgatggcgag cccaaccgcg agtttggccg cgtggtcgtc
      541 gatcgccgcg cgttcgccat cgccgaagac gagtttgcca acatcatcag ccaccccgac
      601 gggcggatga aggaaatcag cattcagcag aatgaggtgc agtggcagga cgacatcgtc
      661 ctgcactatt cgtccgacac gcagccgggc agctccgggg ccagcgtctg caacaacaac
      721 tggcagctgg tggccctgca ccacgccagc cagaactcga acgtgcccga gttccaactg
      781 ctcaacgagg ggatcaagct ctcggcgatc gccgccgacc tggagcggcg ggcgtcggcg
      841 ggaaactcgg acgccccgct ggcccgcgag ctcgtggcgc tgtttcgcgg gaccgacgaa
      901 cggctgggct ttttcggcgc acttggtcgc cgcaccgagt cgcaggggct ggaggcagtc
      961 gttgacgctt accaaggaac cgagcaggac gtcgacatcg gcttctggaa cgtagaatgg
     1021 ctcacgaacc gctacgacga aaaagcacag gcggtcgcgc gggtgatgaa ggaattcaac
     1081 ctcgatgtct ggtgtctgga ggagtcgtcg cccaacgcgg ccgaggcggt cgtggccgag
     1141 ctgcggaacc tgggcctcga ttttgctacg ctgcccgccg agcccgcttc ggccgacggc
     1201 aagcagagct gcacgctgct ctggaaccgc gccacgctca actgtgttca ggaagaatgg
     1261 ggacagccga tcgaagagtg gctgcggtcc cgcagcacgg agtttgacga cctgggcctg
     1321 gaggccgtcc acggcaggat ttttgaccgc tacccggcgc tcttcaagct cacgacggtg
     1381 cagccggtca acggcaatcc ggtcgccttc catctcgtgc cggtgcacct gaaagcgatg
     1441 gaagagggca gcctgcggcg gcaaatggcg tcgaaaatcc tggcggccgc ggcggcgcgg
     1501 aaaattgccg acggcgccgg ggaggactgg attttcggcg gcgacttcaa tgccgagctg
     1561 gccacagacg atttcgacgc cctcgtgaca ggcggcatgg tcccgatcag cgccaccgac
     1621 gaggcgggcg gcgcattttc ctatattaag gggccgcgtt cgctgatcga tcacatcttc
     1681 ctgtcgccga atcttgccga gcggtatggc gcccaggact ttttcatcgt ggccgccgac
     1741 cgcacgctgc cggattacgt gtccgacatc agcgaccatc ggccggtcct gtttcggatg
     1801 agctttcaag gcggcggcga agcggcgcct cccgctccgc cgctcgaagc cgcagtcagc
     1861 gattcgcccg ccatcgccga gctcaagcgg atgctcgcgg ctgcagcgca ggaaccagcg
     1921 gccgaagaag ccctggatct cgaagccaaa cggaagaaaa agccgaagtc gggcgccgtg
     1981 cgcggcggcg agcggcgggc cagcgactac gacgatcgcg acggctatga tgcggcgttt
     2041 ctcggcaacg gctcgcaccg cgtccccgtt ccggagttgt cgtccacgct gtcgtctcac
     2101 gccgttgtcg tagcgcccgg ggcggcgggg agcgcccggt acctgctgcc ttacacgcac
     2161 ttttcactgg tcatgcacgg ccagcggaag ctgccaattc tgacggccgt gaacatcgac
     2221 ggcacgcagt cgctgaagat cgcccgcgag ggcgacaagt ggtatctcga tccgcggatc
     2281 ccgaccgagg cccagatcgg caacgaactg tacacgaaca acgatctcga tcgagggcac
     2341 atggtgcggc ggctggatcc ggtgtgggga cgccgtgcgg tcgcgaccgt ggccaacgac
     2401 gatactttcc actacaccaa cgcttgcccc cagcacaagg acctcaacca gaaggaatgg
     2461 agcgagctgg aggattacat cctcgacaat gcgggggtgc acggcctgcg ggtgtgcgtg
     2521 ttcaccgggc cggttctgcg ggccggtgat cggccgtacc gcgacttggc gctgatcccg
     2581 gaagacttct ggaaagtggc ggtcatcatc cgcgaagata cggggcggat gtcggcgacc
     2641 ggctacgtcc tcacgcaaaa ggacatgatc tcgggtttgg agttcgcgtt tggggcgttc
     2701 cggacttacc aggtttccct gcgctcgatc gagcggatga ccgggctcga cttcggccgg
     2761 ctgaaggacg ccgaccccaa acggcggcgg ggaggcctcg aagccgcggg cggaccggag
     2821 gacgagccac cgacggaggt ggcgggaccg gggtcgctgg tgttctagcg gcacggccga
     2881 cagttagctg tctcaggagt agctcatgaa aggcatcgaa ttcgctgagg tccgcgacgc
     2941 catcacgcgg gccttcaaca gcgacgagtt cgacatgttc ttgttcgagc gttttgactt
     3001 cgaccgggcc gtcaacgtag ccgacggccc gtttcgcgtc gtcgtgcaga acgtgctcaa
     3061 ggacttcatg caaaaaggtc tggacgctgt gctgatcgcc gaagtggccg ctcagcgtcc
     3121 actcaagacc gacgtccagg aggtctatcg caagtacgcc aaggccctgg tcggcgaggc
     3181 ccgccaggaa gaaatcgata ccgagcggct gcaagccttg gagcggtacg gcctggccac
     3241 gaaggtcgtg ttgcaaaaag ccggtcaggc ccaactacct tccgccacga ctccgggcga
     3301 agggtttgag cggacggtca aaaagtactt gccgttcttg aatccgggac agtggcggag
     3361 ggacatgttt cggagggaat gctgcgtttg ccgggtcgaa gtcggcgggg gccagatggg
     3421 gacaggcttt ctcgtcggcc cggacgccct gctgaccaat taccatgtgc tgcaacccgt
     3481 gctcgagggc cagtcgccag cggacaaagt tcgctttcgc ttcgactacc acgtccagcc
     3541 taatggagtg gagtcgccag gaatgctggt ggagttgcac cccgccgaat ggaacatcga
     3601 ccacagccgc tttacggcgg gcgagaaggc ggggaatccc gaccagacgc tacctacgac
     3661 ggacgagctg gactatgcgc tggtcaggct cagccgctct gtgggccagg aacccgtgga
     3721 cggcgagacc gcgaacgggc ccccgcgggg ctggattcag ctacccactg cggctccggc
     3781 catcgtgccg cagatgccaa tcttgatcct ccagcatcct cagaccgagc cgctgaagct
     3841 ggcgatggac acggaaggcg tgctgatggt caacgccaat gagacccgcg tccgctacac
     3901 cacgaacacc gaaggcggtt cgtcgggctc gccctgcttc gacatgaaat ggaatcttgt
     3961 ggcgttgcac cattatggcg acccagccta ccagcaccct gtcacctaca accaaggcat
     4021 tcccatcgac gcgatccgta agcgcctggt caagcaacac aaggaaaacg ccctcggcgg
     4081 tccgcccccg ttctagtgcc gccctccgcg gcgtgtggcc aacgttgcga gcttgaatcg
     4141 ccatgactat caaccaactc atcgtcaggc tggcagagct gatctgcgat gccttcgaag
     4201 aggagcagtt cagcatgctg ctcttcgaga atgttgagga aaaggaattc gactatctga
     4261 gcgatgccaa gggagatact tacaaggccc gtgtcttctt caccgtccgc tgggccaacc
     4321 gcaccggcca ggtcaacaaa gtgctgtggc tggctcagaa agtggccggc ctgcgcgccg
     4381 gccgggccga tttgcacgag ttggcggcgc aaattggtgc ggcggcggcg cctgccgccg
     4441 cggcgccggg agcaaatggt ctgcccaacg aattgtcagt ggggattact ttgctcgagc
     4501 agcagttgcc caagccgctg gaccatgccc gcgatctccg cgacctgctc cgcaagaagg
     4561 attgccgtgc gatcgacttg ccgggccgat ttcatctgag cgaccgcgac atcgagcgac
     4621 tggttaccgt cctggcgcta gaggcggccc cgaccatcag gtacatccgc tggctggcgg
     4681 agcggctggt ggtcgagcct ccattcatcg cttttatggc ggccaaggcg atgacgaccg
     4741 ccatgcttcg actcggcggc gacgaattgc agcgggcgat ctccgcagcg cggaatgccg
     4801 aagacttgct cgacggcctg gtggacgccg acgatgccac gacgaccagc ttcggattgg
     4861 cggcccgcaa ggagcaactc gcctcggctt tgatgctggc cgagatgcgc accaaacaag
     4921 gaaaattcct gctgccggct tccgatctgg aagcattctt gaatgagctg atcacattgt
     4981 tcaaccgtga cacgttcgac ctgatgtgcc gcaacggctt gcaaaccgcc tcgaagttct
     5041 tgggagcgca ccctcacgac ccgatggagt tgattgccat ccaggtattt cttacgtgcc
     5101 gaggaaagtg ggagcgcgaa ctgattcaag cggcgcaaca ggcgcaaccc gcctcgcccg
     5161 tgtttcagaa cctccgccaa aagttcgccg cggccgcggc cggatgagga ctcctatgct
     5221 gacatcgcca caactgcgca agttgcacaa ggcgctgctc gaagcttttt cgctcgacga
     5281 ctttgagagc ctgctgcaat gccgaatcgg cgcccggctg gagctgctcg ccacgccggg
     5341 gtccatgcaa tcgcaagtca gtgaggtcct gaaagttgcc gagcaggccg gctggacgca
     5401 cgagttgatc cgcgccgctg ccgacgcccg gccgaacgat ccgtgttttg cagcgctttc
     5461 ggaggggctc ggcttgggag cggagattcg cgtcggtggc gaatccgcga cgccgaatat
     5521 tacagagtgc aagacagggg cgaaattgcc ggagggcttt gccgctgaac cctggcgcga
     5581 ggagttgccg gcgatggaag cccgcgtctg tcgcgtggaa gtcggcggcg gaatcgtggg
     5641 cacgggcttc ctcgtcggtc ccgatgccgt gctgacttgc tactacgtcg tcgagcacgt
     5701 gattcagcag gaggtctcgc cgactgcggt gcgttgccgg tttgacttcc gccaattgcc
     5761 ggacggccgt acggaaggcc tttccgtccg cttgcattcg gattggctga tcgaccgcga
     5821 ggggcaagag ccgggggagc tggacttcgc gctgctgcgg ctggaaaggc ccctcggagc
     5881 cgagccgctg aaccccgcgg cgccagccga ggtaaaacgg ggttgggttc acctgccggc
     5941 agacctgccg ccggtgccag tcggcgcacc gctgatggtg ttgtcgtacg cgctgggcgg
     6001 cccgctgcaa ctggccctcg aaaccaatgg cttcatcgga ttcgtcgaga acgatcgcct
     6061 cacctaccgg atgtccacgc aacccggcgc gggcggagcg ccctgcttca cttcggactg
     6121 gaaactggtg gccttgcacg aaaagcgcgc gagcgccgat tcggataatc cggagcgctt
     6181 tggcatctct gctgcggcgt tgcgccgaaa aatcgagaag gatgggcact cggccgcgct
     6241 cggccacgca agctccagtg aatctgacac gggctccgtc ctggcaaccg ccaaaccgcc
     6301 caagccgaag cgggtgcgga atgtggacga tccgcaaaag gatcgctggg gcgggaagtc
     6361 caaacggagc ggccgggcgc tccgcgtcga ggacttgctc gcggccaatt ccgcgttcac
     6421 cttcaatgcc gtcgtagcct cgaccgatgg ctcgccgctt gtcggcccgg tgatctttca
     6481 cctgcacgat tcgtatccca agagcgtgct gcgaatccac aagatcgacg aaggcaaccg
     6541 ggccgtgctt gaggaggtca gctcgtacgg cgtctacacg atcggagccc aggtcaagga
     6601 cgcccagggg aagtggatcg gcttagaata ctgcctgtcc gaggtccccg acttgcccgc
     6661 tcgcttcatg aatcgctgaa cgccgctgcc atgcctcctc ttaccggcgc gcaattcgaa
     6721 gagctggccg ctgccctggc cggcgcggac ggcgcggccg ccggcgcatt cgacaaagac
     6781 acgctcgagc aattggtgca gttcaagctc ggccggcggc tgttcgactt cgtggccgag
     6841 gacaagccgc tgaagacgat cgtgtttcag ctgatccgca cggccgagat ggagggctgg
     6901 accgcggcgc tgatccaggc cgtctcgatc acgcggccgg gccacgcgca ggtgcagcaa
     6961 ttcgtcgccg cccactttcc gcaggccgcc gcgccgctct tgccggccga gcaggtcgaa
     7021 agggtcaagc ggggcctcgc cgctctggcg acgatcgtgg aacgcctccg cgacccggaa
     7081 atccggctga tcgtcggccg gttccgggcg gacttcgaaa tcacgcggga aaaggtggcg
     7141 gccctggccg gctacaaggc cctccacgat atgctgcatc aggtggagat gcggtacctc
     7201 agctcgatgg ccgacgccgc gcagctgtgg cggcagagcg aggcccaggt gcaagcgctc
     7261 gatcgctatg cggggcagct gctgggcgac tgcgagcggg cccgccagga cgcgcgggtc
     7321 ctgcgaaact tccgccagga agaggactgg atcgacatgc ttgtccaagc cgcgcggctg
     7381 gtccagcagg ccgtcagcgg cggcgaggaa gcgccgctgg ccagcggact gaacctgctc
     7441 cgcggcgtgc tggtccacaa tccgccgcgg atcaaccacg ttctctgcga ggccgccggc
     7501 gacctgcggc tcaagcaact catcgaggcg atgcaacaaa tcgaggccca gttgggccgc
     7561 acgcagcccg ccgccgcgcc cgacgacgaa ttcgccgccg ggctgcagga gcttcagctc
     7621 ttgcaaccgc gcctgggcgg gctggtcgcg gagcactttg aatggcaatg gctcgatcgc
     7681 gaaattgccc tggccgccac gcagcccgga gtcactcccc aggcccgctt ctttcgctgg
     7741 cccgacttcc agcgccgcct gctggcgctg tgcggcctga gcccgcaaaa ggaatggtcg
     7801 cagcagctgt gcgcaaactt gactgccctg accgaggcgg gctcggccgg cgactccatc
     7861 cagttcgcac gccgcttcaa taaattccgc accgaagcgt ccgtgcgctt cttcaatgtc
     7921 gatgcggagc tgcgcgactt gagcagccaa ttggcacagg tcgcgcagcc gctggaccaa
     7981 ctgctcagga tcgtgaccca tggcaacggc tgaactgcct gtcgagtcgc cccccgcgga
     8041 gccgcttacg tcgctgcggg ccatccgtgc cgcgcacacg gagctgcttc agcaggttcc
     8101 ggccgaccgc atcgatgcgc cgcagatcgc gctcatcgcc gatttcctga atcgatccgc
     8161 cgccgctggc gcattcctcg acttgcccga ggaccgcgct gccgcgcagg gcctgctcga
     8221 ctattggaag tcgtcgttgt attcgcagcg ccgccaggcc gcgttcggtg agggggcggt
     8281 gcgattgccg gccgcgaccc tggccgactt cagcccagcg gcggtggaag aaatcgtcaa
     8341 ggccgccgag gctcgtctgg ttggagacga caaggtgcag gctgcccgga acctgctgct
     8401 caagctgatc caactcccgg ccgaacgcgg cacgccccag accgacgagg tcgagcggag
     8461 caagctcctg gagcgggagc ctcgggccgc agagcaagtg ctcgcggatc tcgaagcggc
     8521 cggagtgctc gaacgtttcg gcgatcctcc gcgcgtgcga ttgcgggccg acgcgctgct
     8581 ccgccgctgg ccgcggctgg ccgcctggct ggaaaagcgg ctgctgtttc gcgaggcagc
     8641 gctgttctgg gaccgccaca accgggaccg cggagcgctc tttacgcggg agctgctcgc
     8701 ccagtcgcac gcctaccacg acaaaaacgc cttggaagcc gagttcgtca aggaggtcaa
     8761 cgccgaagcc gatcggcagg aagatcggca ggaagcgttg catcggcaga aagtgcggac
     8821 ctggcgacgc gttgccttcg cgatgggcgt gatggtgctg ttggacttcg tctgcctggg
     8881 ctacgtggcg gtcaccttca gccagcgcca gcaattggcc atcgagcaaa aggagctggc
     8941 cgaagcgaag ctgcggcaag gacggatgat gaatatggtc ggcttgttgc gggccctggg
     9001 taacatcgcg gccgccgaat cgccggccga acgacaactg gcgatcaaag gttgggaagt
     9061 cctggaggcc cgcggagcgg atgacccgga aattgccgcc ttcctgaaaa gttttgccgg
     9121 ccagattcgc acgattgcca cggaccggca gcggcatgcc aactccgcgg ttcagtcgat
     9181 gcagtacaag gaattacaag gggaagtcct ggagctagcg accgcctttc gcgacgatgt
     9241 tgtcaaatcc cgcgatgacg catcgattca gcggctgagt gaattgaggg aagactacta
     9301 ttccatcatc cgcttcctgg tggagaagat cgtcgcggcc gcgcaatccg gcgatagcgc
     9361 cagcagcgtg caaccctacc ttcgcgaatt ctggcagctc tactggggcg agatgggcct
     9421 ggtcgagggg ccggatgtca aaaccgggat ggttcaattt ggcaacagac tgtggatctg
     9481 gtccgagcac gcggccgggc gtaacgaacc cttcaaggat ctcgatggac ccgcccagcg
     9541 cctcttcgag agcctggaga aagagctgaa gctgccgatt gaaccatcca cgcctccagc
     9601 tgccagcctc tgaacctcgt ggaacaactc gcctccctgg tttgaggcg
//
LOCUS       DASRJB010000663        16333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3858544, whole genome shotgun sequence.
ACCESSION   DASRJB010000663 DASRJB010000000
VERSION     DASRJB010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858544"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1235)
                     /gene="ribA"
                     /locus_tag="VFB80_17580"
     CDS             complement(33..1235)
                     /gene="ribA"
                     /locus_tag="VFB80_17580"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HZN35647.1"
                     /translation="MSDFSSIQSAVDAIARGEVIIVVDAEDRENEGDFICSAEKATPE
                     AINFVLGGRGQFCTPLLPETARRLGLVPVVETNTAPLGTGFLTPVDHRTSKTGITADE
                     RCRTVRALADPATTAADFFRPGHVHPLLAKEGGVLRRAGHTEATVDLCRMAGVAPVGV
                     LCEILDDRGERATRDQLQAIADRHHLKIISIEQLIAHRRVSEKLVSRIAQAALPTGKF
                     GDFTIIAYEVKFETQQPLVLVKGDPAAAAAPLVRLHSSCFTGDLLDSLRCDCGSQLEM
                     ALKMIEGAGVGVLVYLPQEGRGIGLVEKIKAYELQEKGLDTVEANQALGYKADMRDYG
                     VGIQILKDLGLRQVRLLTNNPKKVDAFIYDGFDLKVIDQVPIISPPNPHNARYLSTKR
                     DKMGHILP"
     gene            1323..2735
                     /locus_tag="VFB80_17585"
     CDS             1323..2735
                     /locus_tag="VFB80_17585"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HZN35648.1"
                     /translation="MLRFLPALAALLLIPALTAPAADRPLSAGFAVADITPDVKDGKP
                     VYMAGYGMNRKATGVHDPLYARTVVLGDGDTKFAIVSVDLVGLQFPQTKAIRARLPEF
                     KYVLVGSTHNHEGPDVIGIWGRGPFHRGASEDYLDFVVERVVASVKEAAGKLAPVTAA
                     FGTATDESLMDDSRLPIVKDGVLRLIKLTKAGSSDAAGLLVQWNCHPESLGSKNTLIT
                     ADFPYATVAALEKQYRCPVVYISGAVGGLMSLPGGRIFDAAGKELREGTYEYAEKYGQ
                     EVAALATKAVAASQPIALTPLVASSKSIAVPVQNSLYRAARAVGVLKRDGLVWRGDFH
                     DLSEPMVAEKADLPSAVESEVAYLRLGELHVACIPGELYPELVYGKFQEPVEPDADYP
                     DSPKEPAVAELMPGPKWLLVGLANDEVGYIIPRRQWDKTPPYAYGKDGGQYGEVNSCG
                     ADVAPIIMQALKLRIAEAGK"
     gene            complement(2737..3615)
                     /gene="folD"
                     /locus_tag="VFB80_17590"
     CDS             complement(2737..3615)
                     /gene="folD"
                     /locus_tag="VFB80_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZN35649.1"
                     /translation="MTATILDGKALAATLEAELAEEVADFVQNNGFEPALAAVLIGDD
                     PASGIYVKNKRTACERVGIESQLHRLSSSTTEEELLELVARLNSDEAVSGILVQLPLP
                     PQIREQKVLDAVHPLKDVDCFHPDNVGRLVQGRPRYIPCTPHGCLQILHRHGISVSGK
                     HAVVVGRSEIVGKPLATLLAQKESHLGPDACNATVTVCHSRTRDLGAICRQADILIAA
                     IGKPKFIKGEMVKPGAAVIDVGINRTPEGVVGDVDFAAAREIAGAITPVPRGVGPMTI
                     AMLLSNTLAAARAQLA"
     gene            complement(3815..4390)
                     /locus_tag="VFB80_17595"
     CDS             complement(3815..4390)
                     /locus_tag="VFB80_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35650.1"
                     /translation="MQGSAVQGGAATSALNALGSGNVQGQPVQGQQIQSQQTQVQSQG
                     VQQAQPAQPQQVQSAPAATADASTRSAADMALEALLGGGTQGTVAQEAAPAGAVPTGD
                     FSASISNGTQVRLSLRNDNSFVWVASKGEKQSSFQGSFTLAGTALTLLRSDNQKLEGT
                     LTQNAAGFQLKLAGQNDAGLSFVRAGTLASR"
     gene            4385..4861
                     /locus_tag="VFB80_17600"
     CDS             4385..4861
                     /locus_tag="VFB80_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35651.1"
                     /translation="MHRTALRGLLLSGLSKLALLNRLLLHAGLAELALDLLLTELALS
                     HLALANLALSNLALTNLSLALRELLAKHLLLELLLLRQHRLALGMNRDLGLRLALLLV
                     GLGIVLGLIVSGAITAAATRRAGDAFAGKGREQASARGGEDQRLREAEHDGTPCES"
     gene            complement(5050..6237)
                     /locus_tag="VFB80_17605"
     CDS             complement(5050..6237)
                     /locus_tag="VFB80_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZN35652.1"
                     /translation="MKSSFRFTRILLAVAALAASLAMAVPAWADAKVYEEALQSTTWV
                     LTKTSGKTSSGTGVLVDLEKRLVVTNFHVVGEARTAIIFFPAVENGKPIVARKHYVDN
                     LKTLGIRGKVLGVDRKRDLALIQLDKLPEKGVKAITLAADSAKPGEDVESVGNPGSSD
                     ALWVYTSGKVRSVYRKQFRTGAGDHDFMVVETTSPINTGDSGGPVVNAAGELIAISQA
                     ISPSARLVSYMVDVSEIKLFLTGPWKPAPLPVTDVLTAAELEYSQHTTGNYEIQFEQE
                     DKSKQPVFITKDIEYYEKADTRKIWSLAATLKQAPKLEVAIKLLDQSGRTKLGNWSIE
                     QTQTGEFLVIYLAKIDATASPDAVRSTMEYVAKLTAIMKKELTAKDGKATVTADSLDE
                     WLK"
     gene            6532..8247
                     /locus_tag="VFB80_17610"
     CDS             6532..8247
                     /locus_tag="VFB80_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN35653.1"
                     /translation="MSERLRLLYVCSLGDSGESLPEALRAAADVTTINNPLRAIAAVA
                     REQYDGVYIAGDQLQGAIRLGRLFENDRILEGMPDAVALLDGSLTILWANHLFLEWCN
                     RGNVIGEQFFAALGTTEIVGPEEHPLISALRTSQATSTLLKSGDNKFYQLHAAPIIDP
                     GNQAGHLVVSLRDVTPEISQQQKLAAIQDAGRELADLKPDEIFDMPVEDRIELLKSNI
                     LHCTKDLLKFDVVEIRLMDQKTGELIPLLEVGMDPDAAQRKLFARESGNGVTGFVAAS
                     GKSYLCEDTANDPLYLQGAKGAKSSLTVPLKLHNEVIGTFNVESPQPHAFSDADLQFL
                     QIFCRDVAMSLNTLQLLAAQRMNAAQESVEAIHSQVAVPIDEIVCDATFVTERLDNID
                     PELRSRLEKILTSARGIKDVIQKVGRTLAPADAVPVVGPEKRGKLRGKRVLVVDGDER
                     VRSAAHSLLERHDCVVETAQRGSEAESLFRHGQRDLQYHVVISGVKLPDMSGYDLMMR
                     LAPMINPVPLILTQEFGWDAGHTLVKARQAGLHPSGCVIKPFKERQLLDTLEAIIDWS
                     QGGKA"
     gene            8333..9544
                     /locus_tag="VFB80_17615"
     CDS             8333..9544
                     /locus_tag="VFB80_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN35654.1"
                     /translation="MPFALDLAISLAAFLGHFAIAVWLFNRLHAVGWPRTAIKVLEKV
                     LLAAAALVCALFAFRWLVLGYGLLPPDTGRPPFDHALWLTYAVVCWTAAIAALPAWLI
                     PKLLEKPPAALVASQSHAVDVAARLGFRPVHGAEARFFAAIPGNELLRIEVQRKTLRL
                     PNLPPELAGLSIAHLSDLHMTGQVGREYYDVVVDETNALAPDLIVITGDILEKERCLP
                     WIPQTLGRLQARHGKYFILGNHERRLKDAAVLRAAMVDAGFSDLGGRCETVTLHGVDI
                     LLAGTELPWFGAAPDLQPGTRNPELGTDSLRIPDSAFRILLSHSPDQLPWARLRKFDL
                     VLAGHNHGGQIRLPYLGALIVPSHFGWRYAGGLYDAPPTMLHVSRGLGGIHPIRLNCP
                     PELALLVLQSA"
     gene            complement(9584..10867)
                     /locus_tag="VFB80_17620"
     CDS             complement(9584..10867)
                     /locus_tag="VFB80_17620"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZN35655.1"
                     /translation="MNPAHIIAKKRDGHSLSREEISRFVHEYTRGQIPDYQMSALAMA
                     IYLRGMSAKETADLTDAMLQSGVRLVWPQRERPIVDKHSTGGIGDKTSLVLAPLLASC
                     GLAVPMISGRGLGATGGTLDKLEAIPGFRTNLSLAEIQSTVERVGCVITGASAEIVPA
                     DRKLYALRDVTATVPSIPLITASIMSKKLAESLDALVLDVKFGSGAFMKSQGDARTLA
                     RSLVDTGRRMGVRTSALLTDMNQPLGRMAGNLVEVHESLATLEGRGPADLWDCTRELA
                     AELLVLAMQAADHQSARHMLDQKIASGEALARFREMAQAQGGDLDRLPPLPAAVEIAA
                     ERSGFVLSVNTEQLGHAIIELGGGRKLMSDAIDHAAGLEMCVRIGDRVEEGQPLVRAF
                     GRAEKVERVRELIAGAFAFADQPVPPLPLIVERVE"
     gene            complement(10913..11194)
                     /locus_tag="VFB80_17625"
     CDS             complement(10913..11194)
                     /locus_tag="VFB80_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35656.1"
                     /translation="MTAAEPTRFRWFRRGLLLRGLAIAAALGLLLVLTAATASTPLGM
                     RFAGLFGGIANAIRRHPGGTALVAFGSLAAVWAGLGLLAAVQAFSRRNR"
     gene            complement(11191..11670)
                     /locus_tag="VFB80_17630"
     CDS             complement(11191..11670)
                     /locus_tag="VFB80_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated DNA-protein cysteine
                     methyltransferase"
                     /protein_id="HZN35657.1"
                     /translation="MPKTWAEKLHDAKGLPRVERISGPHKAKYGAGTMLIPAPIEVDE
                     VMRKIPRGKLITINEIRSLLAARHGATITCPITTGIFAWIAAHAADEAAEGGRKRITP
                     YWRTLKSGGQLNPKYPGGLQGIRKLLEAEGHSVVLRGRNLIVEHFEQSLAKVELPGR"
     gene            11819..12697
                     /locus_tag="VFB80_17635"
     CDS             11819..12697
                     /locus_tag="VFB80_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35658.1"
                     /translation="MAQLSWRIPTRGDPRWLRFGLAQVGLATVVAALVLFAAAPRAWL
                     GWALAGLIPLAVFMAYRRWLAYQRSLAGPDNVRIDAAGLHWLDARGQEQSIGRQSVLA
                     FRVGREEDTLRPVPALTLHLAGGFESQPLELHPPATPDAVRDLLGRQWRLAERPAPAG
                     GSLDYDRAIDVYSECHDDFQEWHWEGTREQLTEFFAAVRSAAEQLPPPPLGAKPAQRI
                     ILARRREPLRLRIAHATMPHFDFDLLSAPAAALTSIAAAAEQRLAGAATPSDHKFDVP
                     LGPNDIWTFHLHVREA"
     gene            complement(13092..14978)
                     /locus_tag="VFB80_17640"
     CDS             complement(13092..14978)
                     /locus_tag="VFB80_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35659.1"
                     /translation="MARLFIGYALALALVAIGQAEDDQPTKYAFTQPPLAIYGQVADL
                     KLGAAPTLSDVERKLLEKVTRLRSESPAAKELRDESLILEALLFASGCEDAAARARYR
                     EQYEKLVVAAKEATKDAKTDRERGEALMKFLHAGVMSKGYDLDETTLTAIFDQGKFNC
                     VSSSAMYYLVGSRLGLKLQLISIPGGPFLPGHAALDLIDGRQRVQVEPTNPDGFDWQT
                     KIKRPGVIVLGFVPDRKQGREVDPLGLAATIYSNRGVELGKSDPPRRLEEARMYASAL
                     ALDPLTSTATNNLTALFTNWGPALADDQKFEEGVRVLALGRSLALETRDLENNLSAVW
                     SQHIKATLEVGRDKDALELIRRAAEMVKSDRDFADVGKWFIIRAQDREDAEGWEAGLK
                     SIESSLGVVPAAEVKKLREWRSSLFRRWSQEHLEKGAFDASVKALARGYAIDAADSEI
                     HSGLGYHTAEALKQLDGPDTAKMVAHYQSLVAQFPKVKKIADAARSHAGSTVSDLAEA
                     GKFAEAQQALARYQPLLADAKDRTEIGVMIFGRWGQDLTEKKEWKAALARLSEGRKAY
                     PKERRLEDGIAATIDAWAEGFINDQKWDEAIRIYDIGLKDYLPGNSHLKRNREFCEAK
                     KAGK"
     gene            15182..15793
                     /locus_tag="VFB80_17645"
     CDS             15182..15793
                     /locus_tag="VFB80_17645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN35660.1"
                     /translation="MTKPDVEPTDSANTSTSLLERAVMGRPGAWERLVGLYGPLVYQW
                     CRRWGLQPSDAEALGQEVFLRVFQRLQTHRRDRQEDSFRGWLYRIARNAFVDQIRRGD
                     QPTTAAGGSDALEALHQIPAAAEAEPQKTGEEDALLYRRAVELIRTEISERDWQAFSQ
                     VVLAGRPPVDVAAELNVSPNVVYLAKSRILRRLQEEFADLIDF"
     gene            15871..>16333
                     /locus_tag="VFB80_17650"
     CDS             15871..>16333
                     /locus_tag="VFB80_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35661.1"
                     /translation="MALSSGRLAEPAAAAMLQHINECASCEAVLVRLSAIDDSMARRL
                     REAVAAGGQPLEAECQRMQEAALRIPLAADAAEGAHGPDTTNATHTSTVDSARPQPEP
                     TVTETYSAAAPLPDAIGRYKIRSLLGAGGFGRVFLAADEQLGRLVALKVPKL"
BASE COUNT         2647 a         5414 c         5332 g         2940 t
ORIGIN      
        1 cggaacgcgt gacggcacac ggagtgtgcc tgctacggca ggatgtggcc catcttgtca
       61 cgcttggtcg agaggtagcg ggcgttgtgc gggttgggcg ggctgatgat ggggacctgg
      121 tcgatgacct tgaggtcgaa gccgtcgtag ataaaggcat cgaccttttt cgggttgttg
      181 gtgagcagcc gcacctggcg gaggccgagg tctttgagaa tctggatgcc gacgccgtag
      241 tcccgcatgt cggccttgta gcccagggcc tggttggctt cgaccgtgtc caggcccttc
      301 tcctgcagct cataggcctt gatcttctcg accaggccga tgccgcggcc ctcctgcggc
      361 agatagacga gcacgcccac gcctgcgccc tcgatcatct tcagggccat ctcgagctgg
      421 ctgccgcagt cgcagcggag cgaatccagc aggtcgcccg tgaagcagga agaatgcagc
      481 cgcaccagcg gggccgcggc cgccgccgga tcgcccttga ccagcaccag cggctgctgc
      541 gtctcgaact tgacctcgta cgcgatgatc gtaaagtcgc cgaacttgcc cgtcggcagc
      601 gcggcctggg cgatgcggct cacgagcttc tcgctcacgc ggcggtgggc gatgagttgt
      661 tcgatcgaga tgatcttcag atggtggcga tcggcgatcg cttgcagctg gtcgcgcgtg
      721 gcccgctcgc cgcggtcgtc gaggatttca cacaaaacgc caaccggggc gacgccggcc
      781 atccggcaga gatcgacggt cgcctcggtg tgtccggccc gccggagcac gccgccctct
      841 ttggccaaga gcggatgcac gtgtcccggt cggaagaaat cggccgccgt agtcgcgggg
      901 tcggccagcg cccgcaccgt gcggcagcgt tcgtcggcgg taatgccggt tttcgacgtg
      961 cggtgatcga caggcgtgag gaatcccgtg cccagtggcg ccgtgttcgt ttccacgacc
     1021 ggcacgaggc ccagccggcg ggccgtttcg ggcaagagcg gcgtgcagaa ctgcccccgg
     1081 ccgccgagca cgaagttgat cgcctcgggc gtggccttct ccgccgagca gatgaaatcg
     1141 ccctcgtttt cgcggtcctc ggcatcgacg acgatgatca cctcgccgcg ggcgatcgca
     1201 tcgacggccg actggatgga ggagaaatca gacatcacac cattatagcc gcggctttgg
     1261 aacccattgg agaagttcgc ggtataatgt cgcgtcgccc atccccgctc gcgagtctgg
     1321 ccatgctgcg gtttcttccg gccctggcgg ccttgttgct tatccctgcg cttacggcac
     1381 cggccgcaga tcggccgctg tcggcagggt tcgcagtcgc cgatatcacg cccgacgtga
     1441 aggacggcaa gccggtctat atggccggct acgggatgaa ccgcaaggcg acgggggtgc
     1501 acgacccgct ttatgcccgc acggtcgtgc tgggcgacgg cgacacgaag tttgccatcg
     1561 tctcggtcga tctggtcggc ctgcaatttc cgcagacgaa ggcgatccgg gcccgcctgc
     1621 cggagttcaa gtacgtcctg gtcggcagca cccacaacca cgaaggcccc gacgtcatcg
     1681 gcatctgggg ccgcggcccg tttcatcgcg gcgctagcga ggactatctc gacttcgtcg
     1741 tcgaacgggt cgtggccagc gtcaaagagg ccgccggcaa gctcgcgccg gtcaccgcgg
     1801 cattcgggac cgcgaccgac gaatcgctca tggacgactc gcgcctgccg atcgtcaagg
     1861 acggcgtcct gcggctgatc aagctgacca aggccggcag cagcgacgcc gccgggttgc
     1921 tggtgcagtg gaactgccac cccgagtcgc tcggttcgaa gaacacgctg atcaccgccg
     1981 actttcccta cgccacggtc gccgcgctcg agaagcagta tcgctgcccg gtcgtctata
     2041 tctccggcgc ggtcggcgga ctgatgtcgc tgcccggcgg ccggattttc gacgctgccg
     2101 gaaaggagct ccgcgaaggc acctacgaat acgcggagaa atacggccag gaggtcgcgg
     2161 cactggcgac caaggccgtt gcggcgtcgc agccgatcgc tctcacgccc ctcgtcgcct
     2221 cgtccaagtc gatcgccgtt ccggtgcaga attcgctcta ccgcgcggcg cgagcagtcg
     2281 gcgtgctgaa gcgcgacggc ctcgtctggc ggggcgattt tcacgatctc agcgagccga
     2341 tggtcgccga aaaggccgat ctgccttcgg cggtagagag tgaagtggcc tatttgcggc
     2401 tgggagagct gcacgtggcg tgcattccgg gcgagctcta tccggagctc gtctatggca
     2461 agttccagga gcccgtcgag ccagatgccg actatcccga ctcgccgaaa gagcccgcgg
     2521 tcgccgagct gatgcccggc ccgaagtggc tgctcgtcgg gctggccaac gacgaagtcg
     2581 gctatatcat tccccggcgg cagtgggaca agacgccgcc ctatgcctac ggcaaggacg
     2641 gcggccagta cggcgaagtc aacagctgcg gcgccgacgt cgctcccatc atcatgcagg
     2701 ccctcaagct gcggattgcc gaggcgggga agtagctcac gccagctgcg cccgggcggc
     2761 ggcgagcgtg ttggagagca gcatcgcgat ggtcatcggg ccgacgccgc gcggcactgg
     2821 tgtgatcgcg ccggcgattt cccgggcagc ggcaaaatcg acgtcgccga cgacgccctc
     2881 cggcgtgcgg ttgattccca cgtcgatcac cgccgctcct ggcttgacca tctcgccctt
     2941 gatgaacttc ggcttgccga tcgccgcgat caaaatgtcc gcttgccggc agattgcccc
     3001 caaatcccgc gttcggctgt ggcagacggt caccgtcgcg ttgcaggcgt ccgggccgag
     3061 gtggctctcc ttctgggcca gcagcgtcgc cagcggcttg ccgacgatct cgctgcggcc
     3121 gacgacgacg gcgtgcttgc cgctgaccga aatgccgtgc cgatgcagaa tttgcaggca
     3181 gccgtgcggc gtgcagggga tgtagcgcgg gcggccctgc acgagccggc cgacgttgtc
     3241 cgggtggaag caatcgacgt ccttgagcgg atggacagcg tcgaggactt tttgctcgcg
     3301 aatctgcggc ggcagcggca gctggacgag gatgccgctc accgcctcgt cgctgttcag
     3361 cctggccacc agctccagca gctcttcctc ggtcgtggag ctgctcaggc ggtgcagctg
     3421 gctctcgatg ccgacgcgct cgcaagcggt ccgcttgttc tttacataga ttccactggc
     3481 cgggtcgtcg ccgatgagca ccgcggccag cgccggctca aacccgttat tctgcacgaa
     3541 atcggcaact tcctcggcca attccgcctc cagcgtcgcc gccagcgctt tgccgtccag
     3601 aatcgtcgcg gtcaaatcga ggactccttg aacccgaagc aggccccgcc cggcaggcgg
     3661 gacctgcagc ctctgcatcg ttgacgacgc tgcctgcgcc gtcaacgccg ggtcgggaaa
     3721 gcgaaaagac cgccgccaag aaacagcgac gccgcccgca actttcgtcc gcgggcggcg
     3781 tctggttgat tagccgcgtc ctgttggtgt tccgttaccg gctggcgagc gtgccggccc
     3841 gcacgaagga cagcccggcg tcgttctggc cagcgagctt cagctggaag ccggcggcgt
     3901 tctgcgtcag cgtgccttcg agcttctggt tgtcgctgcg gagtagggtc agagccgtac
     3961 cggccagcgt gaagctgccc tggaagctgc tctgcttctc gcccttgctg gcaacccaga
     4021 cgaacgagtt gtcgtttcgc aggctcaggc gaacctgcgt gccgttgctg atcgaagccg
     4081 agaagtcgcc ggtcggaacc gcaccagccg gggcggcttc ctgagccacc gtgccttgcg
     4141 tgccgccgcc cagcagcgct tccagggcca tgtcggcggc cgagcgagtc gaggcatcgg
     4201 ccgtggcggc cggagcgctc tgcacttgct gcggctgagc gggctgagcc tgctgcacgc
     4261 cctgcgactg gacctgagtc tgctgcgatt gaatctgctg gccttggacc ggctggcctt
     4321 gaacgttgcc gctacccagg gcattcagag ccgaggtcgc cgcaccgcct tggactgcgc
     4381 tgccttgcac cgcaccgccc tgcgcgggct gctgctgagc gggctgagca aactggccct
     4441 gttgaaccga ctgctgctgc acgccggcct ggccgaactg gccctggacc tgctgctgac
     4501 cgaactggcc ctgagccacc tggccttggc caacctggcc ttgagcaacc tggccctgac
     4561 caacctgagc ctggccctgc gggaactgct ggccaagcat ctgctgctgg agctgttgct
     4621 gttgcgtcag caccggctgg ccctggggat gaaccgtgat ctggggctga ggctggcact
     4681 gctgctggta ggcctgggga tagtactggg gctgatagta agcggggcga taaccgccgc
     4741 cgccacgcgg cgggcaggcg atgccttcgc cggcaaagga cgtgaacaag ccagcgcccg
     4801 cggcggcgag gaccagagac ttcgggaggc ggaacatgac ggaactcctt gtgaatcctg
     4861 agtttagtgt tggacaggac gcggcttgcg tctgtctagc taagcgaaaa aagcggggcg
     4921 actgttacag cacagtcgcc ccgcctgggg tttatttgca gccgacgctt cagcgtgggc
     4981 gttgtaccta cttcgaggga gagccagagc tcgccccagt ccgccgacgc tggcagcgtc
     5041 ggctacgtct tacttcaacc attcatccag gctgtccgcc gtgaccgtgg ccttgccgtc
     5101 cttcgcagtc agctccttct tcatgatggc ggtcagcttg gcgacgtatt ccatcgtgct
     5161 gcggacggcg tcgggcgagg ccgtggcgtc gatcttggcg aggtagatca ccaggaattc
     5221 gccggtctgc gtctgctcga tgctccagtt ccccagcttg gtccggccgc tctggtcgag
     5281 cagcttgatc gcgacttcca gcttcggggc ctgcttcagc gtggcggcca gcgaccagat
     5341 cttgcgggtg tcggcctttt cgtagtactc gatatccttg gtgatgaaga ccggctgctt
     5401 ggacttgtct tcctgctcga actgaatctc gtagttgccc gtcgtgtgct gggagtattc
     5461 cagctcggcg gcggtcagta cgtcggtcac cggcagcggg gcgggcttcc aggggccggt
     5521 caggaagagc ttgatttcgc tgacatctac catatagctg accaggcggg cggagggtga
     5581 aattgcctgc gaaatcgcga tcagctcgcc ggccgcgttg accaccgggc cgccgctgtc
     5641 gcccgtgttg atgggcgagg tcgtctcgac gaccataaag tcgtgatcgc cggcgccggt
     5701 gcggaactgc ttccgataga ccgagcggac tttcccggac gtatagaccc acagggcgtc
     5761 ggacgagccg gggtttccca ccgattcgac atcttcgccc ggcttggcgc tgtcggcggc
     5821 cagggtgatt gccttgacgc ctttttccgg cagcttgtcg agctgaatca gggccaggtc
     5881 gcgcttgcga tcgacgccca ggactttacc ccggatgccg agggtcttga ggttatcgac
     5941 gtagtgcttg cgggcgacga tcggcttccc gttttccacc gccgggaaga agatgatcgc
     6001 ggtgcgggcc tctcccacga cgtggaagtt cgtcacgacg agccgcttct ccagatccac
     6061 cagaacgccc gtgccgctcg acgttttgcc gctggtcttg gtcaagaccc acgtcgtcga
     6121 ttggagggct tcttcataga ccttggcgtc ggcccaagcg ggcacggcca tggccaggct
     6181 ggcggccaac gcggccacgg ccagcaagat tcgcgtgaat cggaaagagg acttcatgat
     6241 cgcaccggtt cctgcactga aataggtgag cttggacgtt ggtgatgggc aaatgccatc
     6301 cgctccccct cgaagcgtca aacggggggc gaatgttacg gcaatcgcca gcaaagcccc
     6361 attaccggcg acaatggtct ccgccccggc actgtttgcc tgaattcccg gctaagggcg
     6421 gctcaagtcc cgctgcgacc catccatttg cgttgttgtt tttgcgagat tgtcctataa
     6481 ttccatagac tttcgcttcg cgcatgacgt tttcgaaagc aggagggggc cgtgtcggag
     6541 cggctgcgct tgctctatgt atgcagcctc ggcgactcgg gcgagtcgct gccggaggct
     6601 ctgcgcgccg cggccgacgt caccacgatc aataacccgc tgcgggccat cgcggcggtc
     6661 gcccgcgagc aatacgacgg cgtctacatt gccggcgatc agctgcaagg cgccatccgc
     6721 ctcggccggc tgttcgagaa cgaccgcatc ctggaaggca tgcccgacgc ggtcgcgctg
     6781 ctcgacggtt cgctcacgat cctgtgggcg aaccacctgt tcctggagtg gtgcaaccgc
     6841 ggcaacgtga tcggcgagca gttcttcgcc gccctgggca cgaccgagat cgtcggcccg
     6901 gaagagcacc cgctcatctc cgccctccgc acttcgcagg ccaccagcac gctgcttaag
     6961 tcgggtgaca acaagttcta ccagctgcac gccgcgccga tcatcgatcc gggtaaccag
     7021 gccgggcacc tggtcgtgtc gctgcgagac gtcacgcccg aaatctcgca gcagcaaaag
     7081 ctggcggcga ttcaggacgc cggccgcgaa ttggccgacc tcaagcccga tgaaatcttc
     7141 gacatgcccg tcgaggaccg gatcgagctg ctcaagtcga acatcctcca ctgcacgaag
     7201 gacctgctga agttcgacgt cgtcgaaatc cgcctgatgg accagaagac cggcgagttg
     7261 atcccgctcc tcgaagtcgg catggacccc gacgccgccc agcgcaagct gtttgcccgt
     7321 gaatcgggca acggcgtcac cggcttcgtc gccgcctcgg gcaagagcta tctctgcgaa
     7381 gacacggcca acgacccgct ctatctccag ggtgcgaaag gggccaagag ctcgctgacc
     7441 gtgccgctca agctgcacaa cgaagtgatc ggcacgttca acgtcgagag cccgcagccg
     7501 cacgcgtttt ccgacgccga tttgcagttc ctgcagattt tctgccgcga cgtggcgatg
     7561 tcgctgaaca cgctgcagct gctggcagcc cagcgaatga acgcggccca ggaaagcgtc
     7621 gaggcgatcc acagccaggt cgcagtgccg atcgacgaga tcgtctgcga cgccaccttt
     7681 gtcaccgagc ggctggacaa catcgatccg gagctgcggt cgcgactgga gaagattctc
     7741 actagcgccc gcggcatcaa ggacgtgatc cagaaggtcg gccgcacgct cgcgccagcc
     7801 gacgcagtgc ccgtggtcgg ccccgagaag cggggcaagc tccgcggcaa gcgagtgctc
     7861 gtcgtggatg gcgacgagcg ggttcgcagc gcggcccact cgctgctgga gcggcacgac
     7921 tgcgtggtcg aaacggccca gcgcggcagc gaggccgaat cgctcttccg ccacggccag
     7981 cgcgacttgc agtaccacgt cgtcatctcc ggcgtgaagc tgcccgacat gagcggctat
     8041 gatctgatga tgcgcctggc tccgatgatc aacccggtgc cgctcatcct cacgcaggag
     8101 ttcggctggg acgccggcca cacgctggtc aaagcccgcc aggcgggcct gcatcccagc
     8161 ggctgcgtga tcaagccctt caaggagcgg cagctgctcg acacgctgga ggcgatcatc
     8221 gactggtcgc agggcggcaa ggcgtagcga ctcgcttccg tagccgatct cgccagagat
     8281 cggagcttcg cacgaaatcc gaattctggc gaattcggct accatcggtc ggatgccctt
     8341 cgccctcgat ctggcaatct cgctggccgc gttcctcggc cactttgcca tcgcggtctg
     8401 gctcttcaat cgcctgcacg ccgtcggctg gccgcggacg gcgatcaagg tgctggaaaa
     8461 agtgctgctg gcggcggcgg cactggtctg cgccctgttc gcgtttcgct ggctggtgct
     8521 cggctatggt ctgttgccgc ccgacaccgg ccgcccgccg ttcgaccacg cgctctggct
     8581 gacgtatgcc gtcgtctgct ggacggccgc gatcgccgcg ctgccggcgt ggctgattcc
     8641 caagctcctg gagaaaccgc ctgcggcgct tgttgccagt caatcgcacg ccgtcgatgt
     8701 cgccgcgcgg ctcggcttcc gccccgtgca cggcgcggag gcccgcttct tcgcggcgat
     8761 ccccggcaac gaactcttgc gcatcgaagt tcagcgcaaa acactgcgac tgccaaatct
     8821 gccgccggaa ctcgccggcc tgtcgatcgc acacctttcc gatctgcaca tgacgggcca
     8881 ggtcggccgc gaatactacg acgtggtcgt ggacgagacg aacgccctcg cgcccgacct
     8941 aatcgtcatc accggcgaca tcctcgaaaa agaacgctgc ctcccttgga ttccacaaac
     9001 gctcggccgc ctgcaggccc gccacggcaa atacttcatt ctcggcaatc acgaaaggcg
     9061 attgaaggac gccgcggtgc tgcgggcggc tatggtggac gcgggttttt cggacctggg
     9121 tggtcgttgt gaaacagtga ccttgcacgg cgtagacatt ttgctggccg gtaccgagct
     9181 gccgtggttc ggcgccgcgc cggatctcca acccggaact cggaacccgg aactcggaac
     9241 tgattcactc cgcattccgg attccgcatt ccgcattctc ctttcccatt cccccgacca
     9301 gcttccctgg gcccgcctcc gcaaattcga cctggtgctc gccgggcaca accacggcgg
     9361 ccagattcgc ctgccgtacc tcggcgccct gatcgtcccc agccatttcg gctggcgcta
     9421 tgccggcggc ctctacgacg ctccgcccac catgctccac gtcagccgcg gcctcggcgg
     9481 cattcacccc atccgcctca actgcccgcc ggaactggca ctgctggtcc tgcaatccgc
     9541 ttagccccgg cccccaggcc ggcgtggccg ccggggctaa gcattactcc acgcgctcga
     9601 caatcagcgg cagcggcggt acgggctgat cggcgaaggc gaatgcgccg gcgatcagtt
     9661 cgcggacccg ctcgactttt tcggcgcgcc cgaacgcgcg caccagcggc tggccttcct
     9721 cgacgcggtc gccgatgcgg acacacattt ccaggccggc ggcgtggtcg atcgcgtcgg
     9781 acatcagctt gcggccgccg cccagctcga tgatcgcgtg gccgagctgc tcggtgttta
     9841 ccgagagcac gaagccgctc cgctcggccg cgatctccac ggcggccggc agcggcggca
     9901 ggcggtcgag atcgcccccc tgggcttggg ccatctcgcg gaacctggcg agggcctcgc
     9961 cgctggcgat tttctggtcg agcatgtgcc gcgccgactg atggtcggcg gcctgcatcg
    10021 ccagcacgag cagctccgcg gccagctcgc gcgtgcagtc ccacaaatcg gccggaccgc
    10081 ggccttcgag cgtcgccagc gactcgtgca cctccaccag gttgcccgcc atccgtccca
    10141 gcggctgatt catgtcggtt agcagagcgc tggtccgcac gcccatccgc cggccggtat
    10201 cgaccagcga gcgagccagc gtgcgggcgt cgccctgcga tttcatgaac gccccgctgc
    10261 cgaacttcac gtcgagcacc agcgcgtcga gcgactcggc cagcttcttg ctcatgatgc
    10321 tggccgtgat cagcgggatg ctgggcacgg tcgccgtgac gtcgcggagg gcatagagct
    10381 tgcgatcggc cggaacgatc tcggcgctgg ctccggtaat gacgcagccg acgcgctcga
    10441 ccgtgctttg aatctcagcc agcgacagat tggtccgaaa gccgggaatc gcttcaagct
    10501 tgtcgagcgt gcccccggta gcgcccaggc cgcggcccga gatcatcgga acggccagac
    10561 cgcacgaggc caaaagcggc gcgaggacga gggacgtctt gtcgccgatg ccgccggtcg
    10621 agtgcttatc gacgatcggc cgctcgcgct gcggccatac cagccgcacg ccgctttgca
    10681 gcatggcgtc ggtcaagtcc gcggtttcct tggccgacat cccgcggagg tagatcgcca
    10741 tcgccagggc cgacatctgg tagtcgggga tttggccgcg agtgtattca tggacaaagc
    10801 ggctgatctc ctcgcggctg agcgagtgcc catcccgttt tttggcgata atgtgcgcgg
    10861 ggttcacggt gcgaatctcg gctcattcca tcttagctgg gcggccgtcg gctcagcgat
    10921 tccgccggga aaacgcctgg actgcggcca gcaaacccaa gccggcccac accgccgcca
    10981 ggctgccgaa ggccacgagc gcggtgccgc cggggtgccg gcggatggcg ttggcgatgc
    11041 cgccgaacag gccggcgaag cgcatgccca gcggcgtgct cgccgtggcc gccgttagca
    11101 cgagcaagag cccgagggcc gctgcgatcg ccagtccgcg gagcagcagg ccgcggcgga
    11161 accagcgaaa acgtgttggt tcggcggcgg tcatctgccg ggcagctcca ccttagcgag
    11221 cgactgctcg aagtgttcga cgatcaggtt ccgcccgcgg aggacgacgc tgtgcccctc
    11281 ggcttcgagc agcttgcgaa ttccttgcag gccgccggga tatttgggat tcaactgccc
    11341 gccggacttc agcgtccgcc agtagggcgt gatccgtttg cggcctccct cggcggcctc
    11401 gtcggcggcg tgggcggcga tccaggcaaa aatgccggtg gtaatcgggc aggtgatcgt
    11461 cgctccgtgc cgggccgcca ggaggctgcg aatctcgttg atcgtgatca gcttgccgcg
    11521 ggggatcttg cgcatcacct catcgacttc aattggggcg gggatcagca tcgtcccggc
    11581 gccgtacttc gccttgtgcg gtccgctgat ccgctcgacg cggggcagcc ccttggcatc
    11641 atggagcttt tcggcccagg tttttggcat cagggtctcc cgctttgacc ccaatcgtag
    11701 ccaaaccgga agcaaattcc aaaggaccga agaaccaaga accaaaaacg aacagagcgc
    11761 ttacatttgg ttcttcggtt cttcggttct tcggttcttc ggttcttcgg ttcttcctat
    11821 ggctcaactc tcttggcgta ttccgactcg cggcgatccg cgctggctgc ggttcggcct
    11881 ggcccaggtt ggactggcga cggtcgtggc ggcgctggtc ctgtttgccg ccgcgccgcg
    11941 ggcctggctc gggtgggcgc tggccgggct gatccccctg gcggtgttca tggcctatcg
    12001 ccgctggctg gcctatcagc ggtcgctggc cgggccggac aacgtgcgga tcgacgcggc
    12061 gggcctgcac tggctcgatg cccgcggcca ggagcaatcg atcggccgcc agagcgtgct
    12121 ggccttccgc gtgggccgcg aggaagacac gctgcggccc gtgccggcgc tgacgctgca
    12181 cctggccggt ggcttcgaaa gccagccgct ggaattgcat ccgccggcga cgcccgacgc
    12241 cgttcgcgac ctgctcggcc gccagtggcg gctcgccgag cggcccgcac cggccggcgg
    12301 atcgctcgac tacgaccggg cgatcgacgt ctacagcgaa tgccacgacg acttccagga
    12361 atggcattgg gaggggacgc gcgagcaact caccgagttc ttcgccgcgg tccgctcggc
    12421 agccgagcag ttgccgccgc cgcccctggg cgccaagccg gcgcagcgga tcatcctcgc
    12481 tcgccgccgc gagccgcttc gcctgcgaat cgctcacgcc acgatgccgc atttcgattt
    12541 cgacctgctc agcgcgcccg ccgcggcgct gaccagcatt gcagccgcag ccgagcagcg
    12601 gctggccggc gccgcgactc cgagcgatca caagttcgac gtgccgctcg gacccaacga
    12661 catttggacg tttcacctgc acgtccgcga ggcgtagctc caggaggcgt cggctgccct
    12721 taagagcccg cggcgaatat aacgaaaact gccgcgagca ccgattctcg tcgcgcctcc
    12781 ctgcgttgcc gctcgcgccg caactgcctg acacgcgagg acttccggcg aagccgccgg
    12841 cggtggatcg cataaggctt tgaagcgtta gagagatggc ggcgagtttt cgttatattt
    12901 tgccgccgag cggagtggcg gtgattccgc cacagttgcc aacgaccaaa ttgcgagaga
    12961 gcgggtgaca tttgctcgcg gacggccaaa atggcagtcc gccgcgccaa cgggaactat
    13021 actgcaaata gcgtaccaat gttcagatcg acgtgcgatt cgactccgac caccaaaagc
    13081 cgatagacgg actacttgcc ggccttcttg gcttcgcaga attccctatt gcgtttcaga
    13141 tgcgagttgc cgggcaggta gtccttcagg ccgatgtcgt agatgcggat ggcctcgtcc
    13201 cacttctggt cgttgatgaa gccctcggcc caggcgtcga tcgtggcggc gatgccatct
    13261 tccaggcggc gctccttggg atacgctttc cgcccttcgg acagcctggc cagcgcggcc
    13321 ttccattcct tcttctcggt gaggtcctgg ccccagcggc cgaagatcat cacgccgatc
    13381 tcggtccggt ccttggcgtc ggccaggagc ggctgatagc gggccagggc ctgctgggcc
    13441 tcggcgaact tgccggcctc ggccaggtcg ctcaccgtgc tgccggcgtg cgagcgggcg
    13501 gcgtcggcga tcttcttgac cttgggaaac tgcgcgacga gcgactgata gtgggctacc
    13561 atcttagcgg tgtcggggcc gtcgagctgc ttgagcgctt cggccgtgtg atagcccagg
    13621 ccgctgtgga tttcggagtc ggcagcgtcg atggcgtagc cgcgggccag ggccttcacc
    13681 gaagcgtcga acgcgccttt ctccaggtgc tcctgcgacc agcggcggaa gagactgctt
    13741 cgccactccc gcagcttctt gacttccgcg gcaggcacga cgccgagcga cgattcgatc
    13801 gacttgagcc cagcctccca gccctcggcg tcctcgcggt cctgggcccg gatgatgaac
    13861 catttgccga cgtcggcgaa gtcgcggtcg ctcttgacca tctcggcggc gcggcggatc
    13921 agctcgagcg cgtccttgtc gcggccgact tcgagcgtgg ccttgatgtg ttggctccag
    13981 acggcgctca ggttgttctc caggtcgcgg gtctcgagcg ccagcgagcg gcccagcgcg
    14041 aggacgcgga cgccctcttc aaacttctgg tcgtccgcca acgccggccc ccagttggtg
    14101 aagagggccg tgaggttgtt cgtggccgtc gaggtgagcg gatcgagggc cagggcgctg
    14161 gcgtacatgc gggcctcttc cagccgccgc ggcgggtcgc tctttcccag ctcgacgccg
    14221 cggttggaat agatcgtcgc ggccaggccc agcggatcga cctcgcggcc ctgcttgcgg
    14281 tcgggcacga agccgagcac gatcacgccc ggccgcttga tcttcgtctg ccaatcgaag
    14341 ccgtcgggat tcgtcggctc gacctgcact cgctgccggc cgtcgatcag gtccagcgcg
    14401 gcgtggcccg gcaaaaacgg gccgccggga atcgagatca gctggagctt gaggcccagc
    14461 cgtgagccga cgaggtaata catggccgac gaggagacgc agttgaactt tccctggtcg
    14521 aagatggccg tcagcgtcgt ctcgtcgaga tcgtagccct tgctcatcac gcccgcgtgc
    14581 agaaacttca tcagcgcctc gccccgctcg cgatcggtct tggcgtcctt cgtcgcctcc
    14641 ttcgcggcga ccacgagctt ttcgtattgc tcgcggtacc gggcgcgggc ggctgcgtct
    14701 tcgcagcccg aggcaaacag caacgcctcc agaatcagtg actcgtcccg caactccttc
    14761 gccgccgggc tttcggaccg cagccgcgtc actttctcca gcagcttacg ctccacatcg
    14821 gagagcgtcg gcgctgcgcc gagcttcaag tccgccacct ggccatagat cgccagcggc
    14881 ggctgcgtga aggcatactt tgtcggctgg tcgtcctcgg cctggccgat cgccacgagg
    14941 gcaagggcga gcgcatagcc gatgaacagg cgagccatga tggatctccc caatcgtgga
    15001 acgactcgca actccacagc gtattatcgt agccggatgt tacacgcaac cggttcagat
    15061 agcagcttgc aatcggctga ggtcgacgtc gaatcagcgg gcattgatgc cagtttagcc
    15121 ctgaaactac agtagaagtt caccgtggtc ccgatagcga ccgactcagt tatcacaaat
    15181 cgtgacaaag cccgacgttg aacccaccga tagcgccaac acgtctacga gcctgctcga
    15241 acgggccgtc atgggacggc ccggcgcgtg ggaaagactg gtgggtttgt acggaccgct
    15301 cgtttatcag tggtgtcgcc ggtggggact tcagccgtcc gatgccgagg cacttggcca
    15361 ggaagtgttt ctgcgggttt ttcaacggct acagacgcat cgtcgagacc gccaggaaga
    15421 cagctttcgc ggctggctgt accgcattgc ccgcaacgct ttcgtggacc agattcgccg
    15481 aggagaccag cccaccaccg ccgcgggggg cagcgacgca ctggaggccc tgcaccagat
    15541 tccggccgcg gcggaagctg agccccagaa aaccggcgag gaggatgccc tgctctatcg
    15601 gcgggccgtg gagttgattc gcaccgagat ctcggagcgc gattggcagg ccttcagcca
    15661 ggtggtactg gctgggcggc cgccggtcga tgtggccgcc gagttgaatg tgagtccgaa
    15721 tgtggtgtac cttgccaagt cgcgtatcct gcgtcgattg caagaagagt ttgcggactt
    15781 gatcgacttt tagcgactat gccgcggacg acagaaaata tcgggagccg cgtggcaggc
    15841 cggccggctt gcccgatgga gatcgaggtc gtggcgcttt cctcgggccg cttggccgag
    15901 cccgccgccg ctgcgatgct gcagcacatc aatgagtgcg ccagctgcga ggcggtactg
    15961 gttcgactct cggcaatcga cgattcaatg gcaaggcgct tgcgcgaggc ggtggccgcg
    16021 ggtgggcagc cgctggaagc ggaatgccaa cgaatgcaag aggccgcgct ccgcattccc
    16081 ttggcggccg acgctgccga gggggcgcac ggccccgata cgaccaacgc cacgcacacc
    16141 tcgaccgttg actcggcccg cccgcagccg gagccgacgg tcaccgaaac gtattcggca
    16201 gctgccccgc ttccggatgc gatcggccgc tacaagatcc gctcgctgct gggggcgggc
    16261 ggattcgggc gggttttcct ggccgccgac gaacagctgg gccggctggt cgcgctgaag
    16321 gtccccaaac tga
//
LOCUS       DASRJB010000664         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3016267, whole genome shotgun sequence.
ACCESSION   DASRJB010000664 DASRJB010000000
VERSION     DASRJB010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3016267"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1129
                     /locus_tag="VFB80_17655"
     CDS             98..1129
                     /locus_tag="VFB80_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35662.1"
                     /translation="MSTPRPYHRAQSHWKRGALVGTIVGGLVIAGWQLLPQAQRPPVA
                     QDQQVPSAPVPVERVEQERQARTDWQAAAARLAAAERGQDPGLGQRLNETTALLAVLR
                     SELQAHETQIAVIQKQIASDAPPGEGEGGGVGISPAEAAEAIRLLQTQYEDLAAKLTD
                     DHPKLAAVRQQIADLKSAVAEPSHSAAGATPQRQALEQSLLAEQKQAAALREKERQLV
                     KQQHELRAAAASPSSSAGAEQLKESVKLAEAKHREAAQHLEQARQAATAIRLPAGTNS
                     PASVVTGSPSLTLPVGVVAGLLGVASAAATMLLAARLNRIVTTRRQLAALWDLPVVGT
                     IPAGASRAS"
     gene            1210..2181
                     /locus_tag="VFB80_17660"
     CDS             1210..2181
                     /locus_tag="VFB80_17660"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN35663.1"
                     /translation="MVTELPAAPQVETEHYTPPQTPRACRAAQRPSLQDQALSWIAAG
                     CQMAFGPRERTGLGILTYHRIADPPANRPRPTWNVRPERFQKQIAGLLKRGWQAWPLR
                     QALHYAERELPIPRKTFVVTFDVGYANVLLEACPVLTRLRVPATLFLSTAYLDSQEPF
                     PGDDWEAAGVPGTPTDAWRFLTTDECRRLMGNGLIEIGSHTRHHRDFRDRPVELLADL
                     KDNLNVLKDTFGIDQPSFAPSPVPTDERPTNDELTQAARQAGTICCLTDEPRVIPLKE
                     TPFNWGRFTVSEYDTAATLAGKLGGWHTAVQGLGQDALDHRPRVQGP"
     gene            complement(2224..>3076)
                     /locus_tag="VFB80_17665"
     CDS             complement(2224..>3076)
                     /locus_tag="VFB80_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN35664.1"
                     /translation="NFVKSNKARPFFLYFPSIIPHLALQAPEDAVAEYKGAFPETPYP
                     GGGGYLPNQTPRATYAAMVSRLDKHVGMLVAAVREAGVFDNTIFVFTSDNGPLYGQYA
                     GTDCEFFNSNGGLRGRKGSLYEGGFREPTVIAWKGKIAAGTSSDRVTGFEDWLPTLLE
                     LAGLKDKTPAGLDGISFVPTLLGQSQPPRPFLYREFPAYGGQQCVRIGDWKGVRQNLM
                     PGGAKKAKPAAAPNMRIELYDLKNDWQETKNVSAEHPDVVARIEKLMREQHTPNPEFP
                     FPALDRL"
BASE COUNT          536 a         1021 c         1049 g          470 t
ORIGIN      
        1 cggtgacttc cagtagccgc cgtcgcctcc ggctccgggt taaacgatgg cctaagtaat
       61 cgtgtcccac cgcgcacttt tgcgaagtca caagaatatg tccacgcctc gcccctacca
      121 tcgcgcccag agccattgga aacgcggagc gctggtcggc acgatcgtgg gcggcctcgt
      181 cattgcgggt tggcagttac tgccgcaagc acagcggccg ccggtcgcgc aagatcaaca
      241 agtcccgagt gcgcccgtgc cggtggaacg cgtggaacaa gagcgccagg caaggaccga
      301 ctggcaggcc gcggcggcca ggctcgccgc cgccgagcgc ggccaggacc cggggctcgg
      361 ccagcggctg aacgaaacga ccgcgctgct ggcggtcctc cgcagcgaac ttcaagcgca
      421 cgagacgcaa atcgcggtaa tccagaagca aatcgccagc gatgctcccc cgggcgaggg
      481 cgagggcggg ggtgtcggca tttcgccggc cgaagcggcc gaagcgatcc gcctgctcca
      541 gacgcagtac gaagacctgg ccgcgaaact gaccgatgac catcccaagc tggctgccgt
      601 gcggcagcag atcgccgatc tgaaatcggc agtcgccgaa ccgtcacaca gcgctgccgg
      661 agcgacgcct cagcggcaag cgctcgagca gtcgctgctg gccgaacaga agcaggcagc
      721 cgcgctgcgc gaaaaggagc ggcagctcgt caaacagcag cacgagctgc gggcagctgc
      781 ggcgtcgccc agcagcagcg ccggggccga acaactgaag gagagcgtga aactggccga
      841 ggcgaagcac cgcgaagctg cgcagcattt ggagcaggcg cggcaggcgg cgactgctat
      901 ccgcttgcca gccggaacaa attcgccggc gagcgttgtg acaggaagtc cctcgctcac
      961 gcttccggtt ggtgtggtgg cgggattgct cggcgtggcg agcgcggccg cgacgatgct
     1021 gctggcggcg cggctcaatc ggatcgtgac gacgcgccgg caactggcgg cactgtggga
     1081 tttgccggtc gtggggacga ttccggcggg ggccagccgg gcgagctgaa aattggcccg
     1141 gcgatcttta gcgcggccgc gtatccgcaa ggtacggttt taatggtcgc acccatccgg
     1201 agtcctctca tggtgaccga attgcccgct gcgccgcagg ttgaaaccga acactacact
     1261 ccgccgcaga cgccccgcgc ctgtcgtgcg gcccagcgcc ccagcctgca agaccaggcg
     1321 ctctcctgga ttgccgcggg gtgccaaatg gcctttggtc cgcgcgagcg gacgggcctg
     1381 ggcattttga cctatcaccg catcgccgat ccgccggcca atcgcccgcg gccaacgtgg
     1441 aacgtgcggc ccgagcggtt ccagaagcag atcgcgggcc tgctaaagcg tggctggcag
     1501 gcctggcccc tgcggcaggc cttgcactat gccgagcgcg agctgccgat tccgcgaaag
     1561 accttcgtgg tgacgttcga cgtcggctac gccaacgtgc tgctggaggc ctgcccggtg
     1621 ctgacgaggc tccgcgtgcc ggcgacgctg tttttgagca cggcctatct cgactcgcag
     1681 gagccgttcc cgggcgacga ttgggaagca gcgggagtgc cgggcacgcc gaccgacgcc
     1741 tggcggttcc tgacgaccga cgagtgccgc cgcctcatgg gcaacggcct gatcgagatc
     1801 ggctcgcaca cgcggcacca ccgcgatttc cgcgaccggc ccgtggaatt gctcgccgac
     1861 ctgaaggaca acctgaacgt tctgaaggac acgttcggca tcgaccagcc gtcgttcgcg
     1921 ccgtcgccgg tcccgaccga tgagcggccg acgaatgacg agctgaccca agcggcgcgg
     1981 caagccggga cgatctgctg cttgaccgac gagccgcgcg tcatcccgct gaaggagacg
     2041 ccgttcaact ggggccggtt caccgtcagc gagtacgaca cggccgccac gctggccggc
     2101 aaactcggcg gctggcacac ggccgtgcag ggattgggcc aggatgcgct cgaccaccgg
     2161 ccgcgggtgc aaggcccata gtagtcatct cgctccgcgt tcgcctcacg cggagcgtga
     2221 ggactacaac cgatccagcg ccgggaacgg aaactccggg ttcggcgtgt gctgctcgcg
     2281 catcagcttt tcgatgcggg ccaccacgtc gggatgctcg gctgagacgt tcttggtctc
     2341 ctgccagtcg ttcttcaggt cgtagagctc gatgcgcatg ttcggcgcgg cggcgggctt
     2401 ggcctttttc gcgccgcccg gcatcaggtt ttgccgcacg cccttccagt cgccgattcg
     2461 cacgcactgc tggccgccgt aggcggggaa ctcgcgatac aagaacggcc gcggcggctg
     2521 cgactggccc agcagcgtcg gcacgaagct gatgccgtcg agacccgccg gggtcttgtc
     2581 cttgaggccg gccagctcca gcagcgtcgg cagccagtcc tcgaacccgg tcacgcgatc
     2641 ggacgacgtg ccggcagcga ttttgccctt ccaggcgata accgtcggct cgcggaagcc
     2701 gccctcatag agcgagccct tccgcccgcg gaggccgccg ttggagttga agaactcgca
     2761 atcggtgccg gcgtattggc cgtagagcgg cccgttgtcc gacgtgaaga cgaagatcgt
     2821 gttatcgaag acgccggcct cgcgcacggc ggcgacgagc atgccgacgt gcttgtccag
     2881 tcgcgagacc atcgcggcgt aggtggcccg cggcgtctgg ttcggcaggt atccgccgcc
     2941 accgggataa ggagtttcgg gaaaggcgcc cttgtactcg gcgaccgcat cctccggcgc
     3001 ttgcagggcc aggtgaggga tgatcgacgg aaaatacagg aagaacggcc gggccttgtt
     3061 gctcttcacg aaattc
//
LOCUS       DASRJB010000665        14195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_193904, whole genome shotgun sequence.
ACCESSION   DASRJB010000665 DASRJB010000000
VERSION     DASRJB010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193904"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            749..1789
                     /locus_tag="VFB80_17670"
     CDS             749..1789
                     /locus_tag="VFB80_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZN35665.1"
                     /translation="MPSALLLDNPLRLEIIRLLTSGFTLSDLDVELLGPHLDGLSLAV
                     IGTPGLDHGLEAIMTAVVGYCARRGLIPKLIKGMQAARPLRRDIKTLSPLFVLEPGAP
                     PDEVILEGLVEADCQFMPLKEFVNGLDTITQAVCKVKTPAKDGTGFLVAENLVMTNYH
                     VVQNVHEKANRSVDDVTCQFDFFADAQRFDAAQYRLAAADPADWLAAFSKVGAADPGP
                     EELDFALVRVQPVVGGPLSIRKRLKPAGSEMVVGECLIIVQHPGGNAQRVSINSKSVI
                     ELNQNRSRVKYRTSTLGGSSGSPCFNARLELVALHNSTDPKPVNNWSKYNQGIPFQAI
                     LGKYPDVAKLLS"
     gene            1858..3300
                     /locus_tag="VFB80_17675"
     CDS             1858..3300
                     /locus_tag="VFB80_17675"
                     /inference="COORDINATES: protein motif:HMM:NF039783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35666.1"
                     /translation="MNHVNKAKELLKSSIRTKQRLEQLRTSLVQTQLLPELYLPDELD
                     AIVERFHRDGAGSLLIVAFLSGENLNSPSASQMRKELLTDYSYALAHLAPVLSDERLD
                     HDLVLRRQRAVVGRLQLQNLTEGNPPWGGSWSKLHLAWDLAEFQRLSNGTVPLAEFAA
                     ELLAATQDTVIHGPLQAAIAAANHLLGGALPALGGPADRDRPRVQLYFQLAKGEDASR
                     LTSETRLLAWSWVRWGTGPAQPLFADEFKAKELKWGYPTEFLERVQDRLTRTLGTEEG
                     VPLELFLPLELLGQAVDQWEIDVGSFQPDYVTIGKRHAVVVRVAIPHDPKRPDRADVI
                     RSRFKPRWDAIRGKPLTPADQLADGVLACCWPEEPAPAALGLPALDKHLGLGCIVSRQ
                     TCRAGEPLPLVSHALTRGVPILAWARSDEAAAEMLAAKDKLFCWKPEELPHKIWELRK
                     AGGTDRHLPQHLTLFVEDADHQISNPREFT"
     gene            3297..4301
                     /locus_tag="VFB80_17680"
     CDS             3297..4301
                     /locus_tag="VFB80_17680"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN35667.1"
                     /translation="MSDWKIFHGDRKPHAGIQRLKNTAPPWRNFKKPAAEHRGSTYVA
                     TPTDKLMVNAALYLRRPLLVTGDPGVGKSSLAYAIAYELGLGDVLKWPINSRSTLTEG
                     LYEYDAVARLRDANLEGRSKGSKGRPSEEIHRYLTLKWLGTAFVDSEPRVVLFDEIDK
                     ADVDLPNDLLHVLEDGYFSIPELVRIKDEHPEGVEVATGELSSGETSTIVKEGRVQCQ
                     CFPIIVLTSNRERELPAAFYRRCLRLNIDMENEAEQRLKNIVEQHFKDEPWWTKADGQ
                     QRDEALKDVLAKYLANRRNGPVANDQLLNTLYLIMQGQAPSPSERTVLIDLLMRELSR
                     "
     gene            4298..9931
                     /locus_tag="VFB80_17685"
     CDS             4298..9931
                     /locus_tag="VFB80_17685"
                     /inference="COORDINATES: protein motif:HMM:NF041121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_2336 N-terminal domain-related protein"
                     /protein_id="HZN35668.1"
                     /translation="MTAAEPQAEANVEPQPGPLADVAQALARRIDAAASHLRWPHEGT
                     DVVQAIWLAAQWSTGDEPPPPPPPPPPPVPPPPPGATGKRDSTGDARDSQPPVESRGA
                     ATGERRLLPTVSSPGATTATVAVPVPGEPLAMVRAIRPVARRGPSVARRELDIDATVR
                     RITDEGIWELVLRPVRERIVDLSLVCDVGPSMTLWRETLDALRSALETSGTFRSVRTH
                     YLDTETRPCLIDPASSRILPQAALTRPSIRNPHLILVCSDCVSPAWRRGEVAAQLSQW
                     MRHGTVVLAQTLPEALWDRTALGWSDEHALLPVQWFAENRNLQGRPIDRWASWGFADE
                     AGPEESAAETRPLVPVTWLDPAHLHQLAAWLAGNPARAAGYRLATSAEANKVHPPRDA
                     SELVRDFRQGASRPAVELATCLAVSPLVNLPIMRLIARDLLGERAGRVAEAELWHAGL
                     LRRAQPDQVAEEEEEEAAPLAGRNQELYDFAPGVREELLDALPRVKGREVMLHVSQHI
                     EGSLGRLSGLHALLVDPTQAPTAAGTGTGAWTPAVSSVLNWLGGDYAAAAQRLQLPAA
                     ESRVVELTYSERRAAAAPMQSLEASEPLSRIAWSPRGNLLAAPTRSGQLGLWRPSRKD
                     STIDWQNMSQKGLNEVSWSGDDVTFATACHSHKVLIHRVTDPARPPELLTTLGDHNSE
                     VRCSKFSPDGKWLVTGTAGGTVRFWDPQTFSLLGRHWFAAGKVRALQWLGPSALAVAT
                     AAAVFVLEWESEPRFSKGSPTAHEIRELPVSAASLAWSVATRHFNTETGPLLIAGTLE
                     GQLQFWDVAQKRLRLKVPCGSGRVAGLDLRRVAEGVTLLAVKVHPASASVIHLERGEV
                     IWEREARSEDKSRGGSIVLHPHMPWVAVVTSLDRQIDICKIPIPALPTPEQPSAAPET
                     AREKPWAWVCVAGSGVRASRTDVETARAVGRLLADLDVGLIAGGWQGVDEAVAESFGK
                     ARLAPGKSPDGYFRQIGDPEAALPLTQPRSLEADEEWYRQVLEEGQAVILIGGHSRGG
                     AGTLAERALTRGIPVFPLRKTKGDAGKVHQLMLADWERYRPAGLSREQFLLATDSPHK
                     NLSPLGEALRISLAAPQPPQPSTLPLREPVLINRNQLREHVRQLAAPGGPRVLIVEGP
                     PGSGKSYSLEYIRSFAQSAGGIRVAAFNLLGREPDDIARSIGSAFGADLAAMPQRKTA
                     GDRWGHSVMQWVMASGPSPKDPLWLVIDDVEKDLSPEIRDLILALIDQAAIPPELGEL
                     HIILLGSLDVPAHLEGSVLRERISSFSPADALEYVRQFAQSHYAPIPDANRLEQWVKE
                     VFANLPPEGDPSRHRRLREETERFTSALLEPDAAGIETPPAEQPAETAKPRAVREGKF
                     PTQVLARVTSDVNESQVTSTGFLVGGRLLMTAAHVLFQQRGWATQFTIEFPQRPGMPP
                     IRLRHDPPNSDICRVHSDWSRSGAPECDIGVIFLPEPMGESIDLELAARELKTGMRAE
                     LIGYGTRTPGPSEGPWRAHGQLVDVERRLVRHNMPTLLGSSGSPLLVQGRRDRKAQQV
                     AAVHVSKSQSLGVAVRITPEILELVKQWRAESEARSDRQRDASRPGYDEVFLGKEFEL
                     SLPRLTSAMAALTARRADLGGDWKHSYVLSYAHFSIVMHRQRRLAFYAAANCDFQQQV
                     KIPREASVWVEEPRIDEEAQLDLAFYRGSEFDRRPLARWSDVAWGSHEQAQQAAHDTM
                     HLTNTTPLPGTPLTREIWPRLAEHVAATQSPQDGRVSVFTGHVFLDSDPIFRKVKIPQ
                     SLWRIVAWVNSRNKLCAAAFVMQHDYSGGDEVDPANHPGELVQTTIQHVEALSGLTFD
                     ANLTRADFLFGKEPAKLPAISALDMIRLEPTTDAS"
     gene            complement(9945..11222)
                     /locus_tag="VFB80_17690"
     CDS             complement(9945..11222)
                     /locus_tag="VFB80_17690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN35669.1"
                     /translation="MKIAMLTNTYLPHVGGVARSVSTFQAELTRRGHTVRVIAPQFEG
                     ALDSTDEVLRLAAVQNFNGSDFSVTMPQPGVVAEFLDRMQPELLHSHHPFLVGDTALR
                     AAWTRRLPVVFTHHTMYEQYTHYVPLDSAALRRAAVQMVTEYCNLCTHVIAPSRSIAD
                     LLVERAVTTPISVIPTGIDLDSFASGDGDAFRRRHGVAADAVVVGHVGRLADEKNLGY
                     LARAVGLFLAERPDAVFVVVGSGPSSESLQATVAELAALSQVRMPGTLTGQELFDAYA
                     AMDLFAFSSQSETQGMVLVEAMAAGNPVVALDGPGVRDVMSPDSGVLLAADAPAEDFA
                     QTLAGITRDRAALQQRAERARRSVADFDIKFCADRLLGLYEQLIADFTQRPEADFSPW
                     DRLLRRLEIEWNLIVEKAAAVRAAIAEAPSPPA"
     gene            complement(11219..13540)
                     /locus_tag="VFB80_17695"
     CDS             complement(11219..13540)
                     /locus_tag="VFB80_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35670.1"
                     /translation="MFRFLPWKFVVQRAARAYGLADPIRWLARLRSFAQASEVAEPIE
                     LLRAGILFHARGAVNTKAIQHNLDWIWPYWAQRQFDPRDVSFMPRGFAVSHVNLTHRN
                     WTALGLPELSLYPLVDPRGLLTPLHDGWSLDCWLIDAGGRALVPSRLTDDAVRQELCL
                     QGELAVRTTARLGDLTLEQTAQVVASGGHPAVVLDVTAASPRGGQLVVAIRPYNPEGV
                     QFIDSIAARDGHDGWLVNGRQEVLLDRPAQRLLASDYAGGDVYRRLVDADSAAGAAEP
                     ASVRCPVGMATAAAVFPLAGGPGAVQVRVPLQQELAALGNRRGFHSEVTWTTACAEAA
                     ELRLPDERMQFLYDASVRTLLLLSADEIVPGPYTYRRFWFRDACLMMNALLAIGLVDR
                     CRRAIGRFPARQLASGYFRSQDGEWDSNGQVLWILDRFELLAAEALPDALLTPVQKAV
                     RWVDRKRTKGDGDALHAGLLPAGFSAEHLGPNDYYYWDDFWSEAGLRAAGRIFARHQR
                     QSDAARAVALARELRGAIDRSLARIPAWRSLGGIPASPYRRMDSGAVGSLVADYPLQL
                     FPPGAPPIMATAEALLRRCFVQGGFFQDVIHSGINAYLTLDIAQTLLRAGDARYCELL
                     AAVAGLASPTGQWPEAIHPHTRGGCMGDGQHGWAAAEWIMLLRNCFVREEADRLIVGS
                     GLLPKWTSAGRPLSFGPTLTPWGKVSVRIESAAAAPVLQVTADWHGQPPPVDVEVPGF
                     QRRRLDGGGRLELEPQPASEAGGKPERAQELRS"
     gene            complement(13610..>14195)
                     /locus_tag="VFB80_17700"
     CDS             complement(13610..>14195)
                     /locus_tag="VFB80_17700"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN35671.1"
                     /translation="AKVEVFKRLETDGWNATNITLYSHCGTHMDAPRHFVPGGDPLEK
                     MDLAACCGPARVLDLTPIQPRELIGVSHLQRWHDVIRPGDRLLLRTDWHRRFGTPPYR
                     NELPRIAPELARWLVERRVALLGVEPPSVADVNSLEELTVVHQILLGGNVVIVEGLAW
                     LDQLRSDLVEFIALPLRIVGGDGCPVRAIAIETN"
BASE COUNT         2518 a         4665 c         4708 g         2304 t
ORIGIN      
        1 gacgagcgcg accgttgagc ttggcaatcc gccgccgatt ggttttcagc tccgcggccg
       61 cagattggcg acatggcctc agcggcagct cgccgcctgt ggttgccacg atggtcctgg
      121 acgaggaatt ccgcgtgggc gctcacctgc tttggatgtg cgaccaaatt gaacgtcatg
      181 cgcaaagtgc gcccgtaacg aagctgcacc gctggatagg attgtccaag ccgggattct
      241 agccaatcga aatgctcgat ctagaccaga ccaaggcaat gttcgacgca gtgaagcaat
      301 cgcacggcgg aagcagcgat gatcaaaacc taattgacca tcttgatgtg gacagtgcct
      361 ttaagctgga tcttggcagt catgaccttc cccacgaacc gtggggaagg tcacccgact
      421 tgcagaaccg aagataatgc cctctcccgt cctcgggtga atcacaccgt cggaaagggc
      481 atttcttttt cttgcctgtt ttcgcttctg gtgctacggg aatcgtcttc cattgcttcc
      541 gcgatcgcgc tccagccttt tgcggcaagc cagggcggag ccggagccgc tggaattggc
      601 cggcgtaagg accggcagta gccgagccgg ggaggtttgc gccgtggtta acacgaggtg
      661 tcgaaaccac tagcttgaat tacgtactcc cgctctataa tgggcacgtt tcggctagcg
      721 aggccaatca aaatgggggg cctgtttcat gccgagcgcg cttttgctcg ataatccgtt
      781 gcggctggag atcatccggt tgctaaccag cggattcacc ctcagcgacc tcgatgtcga
      841 actcttgggg ccgcacttgg acggtttgtc gttggccgtc atcgggactc ccggcctgga
      901 ccatggcctg gaagcgatca tgactgcggt cgtagggtat tgtgcccgcc gaggcctgat
      961 tcccaagctg atcaagggaa tgcaagcggc gcggccactg cgccgggaca tcaagaccct
     1021 ctcgccgctg tttgtccttg agccgggagc gcctcccgat gaggtgattc tcgaagggct
     1081 ggtcgaggcg gattgtcagt tcatgccctt gaaggaattt gttaacgggc tcgacacaat
     1141 cacgcaggcg gtctgcaagg tcaagactcc cgccaaagac ggcaccggat tcctggtagc
     1201 cgaaaacctg gtgatgacca attaccatgt cgtccaaaac gtccatgaga aggcgaaccg
     1261 gagcgtggac gacgtgacgt gtcagttcga cttcttcgcc gatgcccaga ggttcgatgc
     1321 cgcgcagtac cgactggcag ccgctgatcc ggccgactgg ctggccgcat tcagcaaggt
     1381 tggcgcagca gatccagggc ccgaggagct cgattttgcg cttgtgcgag tgcagcccgt
     1441 tgtcggcggt ccgttgtcga tccgcaaacg actcaagccc gcgggctctg agatggttgt
     1501 tggcgaatgc ctgattatcg ttcaacatcc gggcggcaac gcacagagag tgtcgatcaa
     1561 ttccaagtcg gtcatcgaac tgaaccagaa ccggagtcgc gtcaaatatc gcaccagcac
     1621 gctgggcggg tcctcagggt ccccctgctt caacgcccgt ctcgaactgg tggcgttgca
     1681 caacagcacg gatccaaagc cggtaaacaa ctggagcaag tacaaccagg gcatcccctt
     1741 tcaggcgatc ctcggcaagt atcccgacgt cgccaagcta ctctcctgac ccgcgctaac
     1801 aagcagcggc cgctcggcac caatcatcta attggcagcg acactcgcat gtggaatgtg
     1861 aaccacgtca acaaggcaaa ggagcttctc aagtcgtcga tcaggacgaa gcagcggcta
     1921 gagcagttgc gcacgagtct ggtccaaact cagctgctgc ccgagctcta cctcccggat
     1981 gagttggacg ccatcgtcga acggtttcac cgcgatggcg cgggttccct cctgatcgta
     2041 gcctttctca gcggcgagaa cctgaatagc cccagcgcca gccagatgcg caaggagttg
     2101 ctgacggact acagctatgc gctggcccac ctggccccag ttctctccga tgagcggctc
     2161 gaccacgatc tggtgctgcg tcgtcagcgc gccgtggtcg gccgcctgca attgcagaac
     2221 ctgactgaag gcaatccccc gtggggcggc agctggtcga agctgcacct ggcgtgggac
     2281 ctggcggagt tccagcgatt gagcaacggc acggtgccgc tcgcggagtt tgcggcggaa
     2341 ctgctcgcgg ccacccagga cacagtcatt cacggtccgc tgcaggcggc catcgccgcg
     2401 gctaatcatt tgctgggcgg tgcacttccc gcgctgggcg gaccggcgga ccgggatcgg
     2461 ccccgggtgc agttgtactt ccagctggcc aagggagaag acgcgtcgcg gttgaccagc
     2521 gagaccaggc tgctcgcctg gtcctgggtg cgctggggca cgggcccggc ccagccgctg
     2581 ttcgccgacg agttcaaggc gaaggagctg aaatggggct acccgaccga gtttctcgag
     2641 cgggtgcagg accggctgac acggacgctg ggcaccgagg aaggcgtgcc cctggagcta
     2701 ttcttgccgc tggagttgtt ggggcaggcg gtggatcagt gggaaatcga tgtcgggtcg
     2761 ttccagccgg actacgtgac gattggcaag cggcacgccg tagtcgttcg cgtggcgatt
     2821 ccgcacgacc ccaaacgccc tgatcgggcc gatgtgatcc gcagccggtt caagccccgc
     2881 tgggatgcga tccgcggcaa gccgctgacg cccgccgatc agctggccga cggcgttctc
     2941 gcctgttgct ggcccgaaga gccggcgccg gcggcgctgg gcctgccggc gctcgacaag
     3001 cacttgggac tgggctgcat cgtctcgcgc cagacttgcc gcgccggcga gccgctgccg
     3061 ctggtcagcc acgcgttgac gcggggggtg ccgattctcg cctgggccag gtccgacgag
     3121 gcagcggcgg aaatgctggc cgcgaaggac aagctgttct gctggaagcc ggaagagctg
     3181 ccgcacaaga tctgggagct gcgaaaggcc ggcggaaccg accggcatct gccccagcac
     3241 ctgacgctgt ttgtcgaaga tgcggatcac cagatttcca atcccagaga attcacatga
     3301 gcgactggaa gatctttcac ggcgaccgca aaccccacgc cggcatccag cggctgaaga
     3361 ataccgcgcc cccctggcgc aatttcaaga agccggcggc cgaacaccgc ggcagcacct
     3421 acgtagccac gcccacggac aagttgatgg tcaatgcggc gctctacctg cggcggccac
     3481 tcctggtaac tggcgacccg ggcgtcggca aatcgtcatt ggcctatgcg atcgcctacg
     3541 agctggggct gggggacgtg ctgaagtggc cgatcaactc ccgctcgacg ctgaccgagg
     3601 ggctgtacga gtacgacgcc gtggcgcggc tgcgggatgc caacctggag ggccgctcga
     3661 agggctcgaa aggccgccca tcggaagaaa tccatcgtta cctgacgctc aaatggctgg
     3721 gaactgcgtt cgtcgatagc gagccgcgag tcgtactctt cgatgaaatc gacaaggccg
     3781 acgtggacct gcccaacgac ctgctgcacg tgctggagga cggctatttt tcgattccgg
     3841 agctggtccg catcaaggac gaacacccgg aaggagtcga agtggcgacg ggcgagctga
     3901 gcagcggaga gaccagcacg atcgttaaag aggggcgggt gcagtgccag tgctttccga
     3961 tcatcgtgct caccagcaat cgcgagcgcg agctccccgc ggccttctat cgccgctgcc
     4021 tccggctgaa catcgatatg gagaacgagg ccgagcagcg gctgaagaac atcgtcgagc
     4081 agcacttcaa ggatgagccc tggtggacca aggccgacgg gcagcagcgg gacgaggcgc
     4141 tgaaagacgt gctggccaaa tacctggcca accggcggaa cggtcccgtg gcaaacgatc
     4201 agctgctcaa cacgctctac ctgatcatgc agggccaagc ccccagccct agcgagcgaa
     4261 ccgtgctgat cgacctcttg atgcgggagc tgagccgttg accgctgccg agccacaggc
     4321 cgaggcgaac gtcgagccgc agccgggccc gctggcggat gtcgcccagg cgctggcccg
     4381 ccggattgac gcggctgcaa gccacctgcg ctggccgcac gagggcacgg acgtcgtcca
     4441 ggcgatctgg ctggcagcgc agtggtccac tggtgacgag ccgccgcctc cgcctccacc
     4501 gccgccaccc ccggttccac cgccgccgcc aggcgcaacc ggaaaacgcg actcgaccgg
     4561 cgacgcacgg gattcgcagc cgcctgtcga gagccgcggc gcggcgaccg gcgagcgtcg
     4621 gctgctgccg acggtaagtt cgccgggagc gacaacggcg acggtggcgg tccccgtacc
     4681 aggagagccg ctggccatgg tgcgggccat ccgaccagtc gcccggcgcg gaccgtcggt
     4741 cgcgcggcgc gagctcgaca tcgacgcgac ggtccgccgc atcaccgacg aagggatctg
     4801 ggagctggtg ctgcggccgg ttcgcgagcg gatcgtcgat ctatcgctgg tttgcgacgt
     4861 tggcccttcc atgaccctgt ggcgcgagac gctcgacgcg ctgcgctcgg cgctggaaac
     4921 gtcgggcacg tttcgttcgg tgcggacgca ctatctcgac acggaaacgc gtccctgcct
     4981 gatcgatccc gccagcagcc ggattttgcc gcaggcggca ctgactcggc cgtccattcg
     5041 taatccgcac ctgattctgg tttgcagcga ctgcgtgtcg cccgcctgga ggcgcgggga
     5101 agtcgcggct cagctttcgc agtggatgcg gcacggaacc gtggtgctcg cccaaacgct
     5161 gccggaggcg ctgtgggacc ggacggcgct gggctggtcc gacgagcacg ccctgcttcc
     5221 ggtgcaatgg tttgcggaga atcgaaacct ccagggccgc cccatcgatc gctgggcgtc
     5281 gtggggcttc gccgacgaag ctggcccgga agagtctgcc gccgaaactc gtccgctggt
     5341 ccccgtgacg tggctggatc ccgcacacct tcaccagctg gcggcctggc tggccggcaa
     5401 cccggcgcgg gccgcgggat atcgcctcgc gacaagtgcc gaggcgaaca aggtccatcc
     5461 gccgcgcgac gccagcgagc tggtgcgcga tttccggcag ggcgcttcgc ggccggccgt
     5521 cgagctggcg acctgcctcg ccgtatcgcc gctcgtcaac ctgccgatca tgcggctgat
     5581 cgctcgagat ctgctcggcg agcgcgccgg ccgcgtggcc gaggccgaat tgtggcacgc
     5641 gggcctcctg cgccgtgccc agccggacca ggtcgccgag gaggaggagg aggaggcagc
     5701 gcccctggcg gggcgcaatc aggagctgta cgactttgct cccggcgtac gggaggagct
     5761 gctcgacgct ctgccgcgcg tcaagggccg cgaagtcatg ctgcacgtct cacagcacat
     5821 cgagggctcg ctcggccgct tgtcggggct gcacgcgctg ttggtagacc ccacgcaggc
     5881 gccgactgcg gcggggactg gcaccggtgc ctggactccc gcggtgagca gcgtgctcaa
     5941 ctggctcggc ggcgactacg ccgcggctgc acaacgcttg caactgcccg ccgccgaaag
     6001 ccgtgtcgtg gagttgacct actccgagcg gcgtgccgct gcagcgccga tgcagtccct
     6061 cgaggccagc gagccgctga gccggattgc ctggtcgccg cggggcaacc tgctggctgc
     6121 cccgacgcgc agcgggcagc tgggcttgtg gcggcccagc cgcaaggatt ccacgatcga
     6181 ctggcagaac atgagccaga aagggctcaa tgaggtgagc tggtctggcg acgacgtgac
     6241 gtttgccacg gcttgccact cgcataaggt cttgattcac cgcgtcacgg atccggctcg
     6301 gccccccgag cttctcacga ccctgggcga tcacaacagc gaagttcgct gctcgaaatt
     6361 ctctcccgac ggcaaatggc tcgtaacggg gacggctgga gggaccgttc gcttctggga
     6421 tccacagacg ttcagcctgc tcgggcggca ctggtttgcc gccggcaagg ttcgggccct
     6481 gcaatggctc ggcccctcag cgctggccgt cgccacggct gcggcggtgt tcgtgctgga
     6541 atgggaatcg gagccgcgct tttccaaggg cagcccaaca gcgcacgaga ttcgcgagct
     6601 gcccgtatcg gctgccagcc tggcctggtc cgtggccacg cggcacttca ataccgagac
     6661 gggcccgctg ctgatcgccg gcactctcga ggggcaactt cagttctggg atgtcgccca
     6721 aaaacgcctg cggctgaaag ttccttgcgg cagcggccgc gtggccgggc tggacttgcg
     6781 ccgtgtcgct gaaggcgtca cgctactggc agtcaaagtg cacccagcca gcgccagcgt
     6841 cattcatctg gagcggggcg aagtaatctg ggagcgcgag gccaggtcgg aggataagtc
     6901 gcgcggaggg tcgatcgtac tgcacccgca catgccctgg gtcgctgtgg tgaccagcct
     6961 ggaccggcaa atcgacatct gcaagattcc tatccccgcg ttgcccactc cggagcagcc
     7021 gagcgcggcg cccgagacag cgcgcgagaa gccgtgggcc tgggtctgtg tcgccggctc
     7081 gggcgtccgt gcttcgcgaa ccgatgtcga gacggcgcgt gcagtcggcc gcctgctcgc
     7141 cgacctggat gtcggcctga tcgccggcgg ctggcaggga gtggacgaag ccgtcgccga
     7201 gtcgtttggc aaagcccgcc tggccccagg taaatcgccg gacggctatt ttcgccagat
     7261 cggcgatccg gaggcagccc ttccgctcac gcaaccacgg tcgttggagg cggatgaaga
     7321 gtggtaccgg caggtcttgg aagaggggca ggctgtcatc ctcatcggcg gccattcccg
     7381 cgggggcgct gggaccctcg ccgaacgcgc gctgacccgc ggcattcccg tctttccctt
     7441 gcgtaagacc aagggagacg ccggcaaagt ccatcagctg atgttggcgg attgggaaag
     7501 atatcgaccc gcgggactca gccgcgagca attcctgctg gcgaccgatt cgccgcacaa
     7561 gaacttgtcg cctctcggcg aagcgctccg cattagcctg gcggctccgc aaccgccgca
     7621 gcccagcacg ctgcctctgc gcgagccggt gctcatcaac cgcaaccagc tgcgcgaaca
     7681 cgtgcggcaa ctggccgcgc ccggcggccc gcgggttctg atcgtggagg ggcccccggg
     7741 tagcggcaag tcatactcgc tcgagtacat ccgctccttc gcccagagcg ctggcggcat
     7801 tcgggtggca gccttcaacc tgctcggccg cgagcccgac gacatcgcga ggtcgatcgg
     7861 ttccgcattc ggcgccgacc tggctgccat gccgcagcgt aagacagcgg gtgaccggtg
     7921 gggccacagt gtgatgcagt gggtgatggc gagcgggccc tccccgaagg atccactttg
     7981 gctcgtaatc gacgacgtcg aaaaggacct ttcaccagag attcgcgatt tgatcctggc
     8041 actgatagac caggccgcta ttccgcctga actcggtgaa ctgcacatca tcctgctcgg
     8101 ctccttggac gtgcctgcgc acctggaggg ttctgtcctc cgcgaacgga tttcgtcgtt
     8161 cagtcccgcc gatgcgctgg agtatgtccg ccagtttgca caatcccatt acgcgccaat
     8221 cccggatgcg aatcgattgg agcagtgggt caaggaagtc tttgcgaatc ttcccccaga
     8281 aggtgatccc tcgcgccaca gacgcctccg cgaagagacc gagcggttca ccagcgccct
     8341 cctggagccg gacgcggctg gcattgagac gcctccagcc gagcagcctg ccgaaacggc
     8401 caagccgcgc gccgtccgcg aaggcaagtt tcccacccaa gtcctggcgc gagtgacgag
     8461 cgacgtcaac gagtcacagg tcacgagcac aggcttcctc gtcggcggcc ggctgttgat
     8521 gacggccgct cacgtgctct tccaacagcg gggctgggcg acgcaattca ccatcgagtt
     8581 tccccagcgg ccggggatgc cgcccatccg cctcaggcac gatccgccga actccgacat
     8641 ttgccgcgta cacagcgact ggtcccgcag cggcgctccc gagtgcgaca ttggtgtaat
     8701 ctttctcccc gagcccatgg gagaaagcat cgatctggaa cttgcggcca gagaactcaa
     8761 aacaggaatg cgcgccgaat tgatcggtta cggaacgcgc acccccggcc cctcggaagg
     8821 cccgtggcgc gcacatgggc aactcgtgga cgtcgagcga agactcgtgc ggcacaacat
     8881 gccgaccctt ttgggttcgt cgggatcccc cttgctcgtg caaggacgga gggatcggaa
     8941 ggcgcagcaa gttgctgccg tccacgtcag caagagccag tcgctgggcg tcgccgtgcg
     9001 gattacgcct gagatccttg agctggtcaa acagtggcgg gccgaatcag aggcccgaag
     9061 tgacaggcag cgcgatgcaa gtcgcccggg atacgacgag gtcttcttgg gcaaagagtt
     9121 cgagctgtcg ctgccgcgat tgacgagtgc gatggcggcg ctgacggcgc gacgggccga
     9181 tctcggcggt gattggaagc actcgtacgt tctctcctac gcgcacttca gcatcgtcat
     9241 gcatcggcag cggcggctgg cgttctacgc ggccgccaat tgcgactttc agcagcaggt
     9301 aaaaatcccg cgcgaggcga gcgtctgggt cgaggaacct cgcatcgacg aggaggcgca
     9361 gctcgacctg gcgttctacc ggggcagcga attcgatcgg cgtccgctgg cgcggtggag
     9421 cgacgtcgcc tgggggagcc atgagcaggc tcaacaggcc gcccacgaca cgatgcacct
     9481 cacgaataca acgccgctac ccggcacgcc gctgacgcgc gaaatctggc cccgcctcgc
     9541 cgagcacgtc gccgctaccc agtcgcctca ggacgggaga gtgtcggtgt tcaccggaca
     9601 cgtctttctc gattccgacc cgatcttccg caaggtcaaa atcccccagt cgctctggcg
     9661 gatcgtcgcc tgggtcaact cgcggaacaa gttgtgtgcg gctgccttcg tgatgcagca
     9721 cgactatagt ggcggcgacg aagttgatcc cgcgaaccat ccgggagagc tggtccagac
     9781 gacgattcag cacgtcgagg cgctcagcgg cctgaccttc gacgcgaatc tcaccagagc
     9841 cgatttcctc ttcggcaagg agccagccaa attgccggcg atcagcgccc tcgacatgat
     9901 ccgcctcgaa ccaacgactg acgccagctg agttcgtccc cccgtcaggc cggcggcgaa
     9961 ggggcttcgg cgatggcggc acggacggcg gcggcttttt cgacgatcag gttccactcg
    10021 atttccagtc ggcggagcaa gcgatcccag ggagaaaagt cggcttccgg gcgctgcgta
    10081 aagtctgcaa tcagctgctc gtagagtccc agcaggcggt cggcgcagaa tttgatatcg
    10141 aagtcggcga ccgagcggcg ggcccgctcg gcccgctgct gcaaggcggc gcggtcgcga
    10201 gtgatcccgg ctagggtctg ggcaaagtcc tccgccggag catcggccgc cagcagcacg
    10261 ccgctgtccg gcgacatcac atcgcgcacg ccgggaccgt ccagggcaac taccggattg
    10321 cccgcggcca tcgcttccac caggaccatg ccctgcgtct ccgactgcga gctgaaggcg
    10381 aacaggtcca tggcggcata ggcgtcgaac agctcctggc cggtcaacgt ccccggcatc
    10441 cggacttgcg acagcgcggc gagttcggcc acggtcgctt gcagcgactc gctgctgggg
    10501 ccggacccga cgacgacgaa caccgcgtcc ggccgctcgg ccagaaacag gcccacggcc
    10561 cgcgcgagat agcccaggtt cttttcgtcc gccaggcggc ccacgtggcc caccaccact
    10621 gcgtccgcag caacgccgtg ccgccggcga aaagcgtcgc cgtcgccgct ggcgaagctg
    10681 tccagatcga tgccggtcgg aatcacactg atcggagtcg taaccgcccg ctccaccagc
    10741 aggtcggcga tgctccggct gggcgcaatg acgtgcgtgc agaggttgca gtactcggtg
    10801 accatctgca ccgcggcccg ccgcagggcc gcggaatcga gcggcacgta gtgcgtgtac
    10861 tgctcgtaca tcgtgtggtg cgtgaagacg accggcagcc ggcgggtcca ggccgcccgc
    10921 agggccgtgt cgccgacgag gaacggatga tggctgtgca gcagctcggg ctgcattcga
    10981 tcaaggaatt cggcgacgac gccgggctgc ggcatcgtca ccgaaaagtc gctgccgttg
    11041 aagttctgca cggcggccag gcggagaact tcgtcggtgg aatccagcgc gccttcgaac
    11101 tgcggcgcga tgacgcggac ggtgtggccg cggcgcgtca gctcggcctg gaacgtgctc
    11161 accgagcggg cgactccgcc cacgtgcggc aggtacgtgt tggtcagcat cgcaattttc
    11221 atgatcgcaa ttcttgcgcg cgctcaggct tcccgccggc ttcgctggct ggctgcggtt
    11281 ccagctccag ccgtccgcca ccgtctagtc gtcgccgctg aaagcccggc acttcgacgt
    11341 cgacgggcgg cggctgccca tgccagtcgg cggttacttg caggactggt gcggcggcgg
    11401 ccgattcgat ccggacgctg actttccccc acggcgtcaa cgtcggtcca aacgagagcg
    11461 gccggcccgc actggtccac tttggcaaca agcccgaccc aacgatcagg cgatcggcct
    11521 cctcgcggac gaaacaattg cggagcagca tgatccactc ggcggccgcc cagccgtgct
    11581 gtccgtctcc catacagccg ccgcgggtgt gcggatgaat ggcttcgggc cattgcccgg
    11641 tcggcgaggc cagtccggcc acggcggcca gaagttcgca gtagcgagcg tcgccggcgc
    11701 ggagcagcgt ctgggcaatg tcgagcgtca aataggcgtt gatgcccgaa tggatgacgt
    11761 cctggaaaaa ccctccctgc acgaagcagc gccgcagcag ggcctcggcc gtcgccatga
    11821 tcggtggagc gcccggcggg aagagttgga gcggataatc ggcgaccagc gagccgacgg
    11881 cgccggaatc catgcgacga tagggagatg ccggaattcc ccccagcgag cgccaggcgg
    11941 gaatgcgggc caggctgcga tcgatcgcac cgcgcagctc gcgggccagg gccacggcgc
    12001 gcgcggcgtc gctttggcgc tggtggcggg cgaagattcg ccccgctgcc cgcaggcccg
    12061 cctcggacca gaagtcgtcc caatagtaat agtcgttcgg acccaggtgc tccgcgctga
    12121 agcccgccgg cagcagcccg gcatgcagcg cgtccccgtc gcccttcgtt cgcttgcggt
    12181 cgacccagcg caccgccttc tgcaccggcg tcagcagtgc gtcgggcaag gcttcggccg
    12241 cgagcaattc aaatcgatcg aggatccaca acacctggcc gttcgagtcc cactcgccgt
    12301 cctgcgaccg gaagtaaccg ctggcgagct gccgcgcggg aaaccggcca atcgcccgcc
    12361 ggcagcggtc caccaggccg attgccaaga gcgcgttcat catcaggcag gcgtcgcgaa
    12421 accagaaccg ccggtaggtg taaggccccg gcacaatctc gtcggccgac aatagcagca
    12481 gcgtccgcac ggaggcatcg tacagaaact gcattcgttc gtcgggcagg cgcagctcgg
    12541 ccgcctcggc acaggcggtg gtccacgtca cttccgagtg aaagccgcgg cggttgccca
    12601 gggccgctag ctcctgctgg agtggaacgc gcacctggac ggcgccgggg ccgcccgcga
    12661 gcggaaacac ggccgccgcg gttgccatgc ccaccggaca gcgcacgctg gccggctcgg
    12721 ccgccccggc ggcgctgtcg gcatcgacca gccgccggta gacgtcgccg cctgcataat
    12781 cggacgccag cagccgctgg gcaggccgat cgagcaacac ctcttgccgg ccattgacga
    12841 gccagccgtc gtgcccgtcc cgcgcggcga tcgaatcgat gaactgcact ccctcggggt
    12901 tgtagggccg gatggccacc acgagctggc cgccccgcgg gctcgcggca gtcacatcga
    12961 gcaccaccgc cgggtgcccg ccgctggcga ctacttgggc cgtttgctcg agcgtcaggt
    13021 cgccgagccg ggccgtcgta cggaccgcca gctcgccctg caggcacagc tcctgccgca
    13081 cggcatcgtc ggtgagccgg ctgggaacga gggcccggcc gccagcgtcg atcagccagc
    13141 agtcgagcga ccacccgtcg tgcagcggcg ttaacagccc gcggggatcg accaggggat
    13201 agagggaaag ctcgggcagg ccgagggccg tccagtttcg gtgggtcaga ttgacgtgac
    13261 tgaccgcgaa gccgcgcggc atgaacgaca cgtcgcgcgg atcgaattgc cgctgcgccc
    13321 aatagggcca aatccaatcc aggttgtgct ggatggcctt ggtgttcacc gcgccgcgag
    13381 cgtggaacag aatccccgcc cggagcagct cgatgggctc ggccacctcc gaggcctgcg
    13441 cgaaactgcg gaggcgagcg agccagcgaa tcgggtcggc caggccatag gcccgcgccg
    13501 ctcgctgcac gacgaatttc caaggaagga agcggaacat gctggtggat ggggctgcaa
    13561 acgacatgcc tcagcgccgg cacaagcagc acgttccgcg cggtcgcggt caattcgttt
    13621 cgatcgcgat ggcccgcacg ggacaaccgt cgccgccgac gatgcgcaag ggcagcgcga
    13681 tgaactccac gaggtcgctg cggagctggt cgagccaggc caggccttcg acgatcacga
    13741 cgttgccgcc gagcagaatc tggtggacga cggtcagctc ttcgaggctg ttgacgtcgg
    13801 ccaccgaggg cggctcgacg cccagcaggg ccacgcggcg ctcaaccagc catctggcca
    13861 gctcgggcgc gatgcggggc agctcgttgc gatagggcgg cgtgccgaag cggcgatgcc
    13921 aatcggtccg tagcagcagc cggtcgccgg gccgaatcac gtcgtgccag cgctgcaagt
    13981 gcgacacgcc aatcagttcc cgcggctgaa tcggcgtcag gtcgagcact cgcgccggcc
    14041 cgcagcaggc tgcaaggtcc atcttttcga gcggatcacc gccgggcacg aaatgtcgcg
    14101 gggcgtccat gtgcgtgccg cagtgcgaat agagcgtgat gttcgtcgcg ttccagccgt
    14161 cggtctccag ccgcttgaag acttcgacct tggcc
//
LOCUS       DASRJB010000666         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3577258, whole genome shotgun sequence.
ACCESSION   DASRJB010000666 DASRJB010000000
VERSION     DASRJB010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577258"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFB80_17705"
     CDS             complement(<1..786)
                     /locus_tag="VFB80_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN35672.1"
                     /translation="MVNRLSMIACLALASAATAPAADARPNILFIIFDDWNGSTHAGA
                     YGCDWIKTPHFDRIAREGVIFKNAFTSNPKCSPCRASILTGRNTWQLKEAVSHNGLFP
                     AGFEVYPDLLEKAGYFVGLTGKGWGPGDFQTQAKRTRNPAGPGFNGLTQEVPASGIGR
                     NDYPGNFAEFLKQRPKEQPFCFWMGFTEPHRAYEFDSGVRLGKKLADVKVPAFLPDTD
                     IVRRDLADYAIEVEWGDQQIGRTLKILEDAGELNRTLVVVTSDH"
     gene            950..2533
                     /locus_tag="VFB80_17710"
     CDS             950..2533
                     /locus_tag="VFB80_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35673.1"
                     /translation="MPIEFSCPACHQLVRTPDAAAGKKGKCPLCGAVVQIPAPAAAPV
                     KPAPAKPAPPKPAAPKPAPKSAPVKPAPVPVPEPEPPAPEPAPDTIEFACVWCNELVR
                     TPASAAGKRGKCPHCQRIVKIPTAERRLVKERDPLLSEELPPMEKVPHGAAPKAAAPK
                     PKPAAKPSTPSVPAARPSVPPPPDLLVPVQPEPAKPAALQPAKPKPPPPEVAAAAGLA
                     EIKVPDVTPITDPQPPPPDPFLGLTPLPSAAEPEGLTPLLDLPKDSFQTLTPLGPDNL
                     TVLTPLPDDPLDGLEASGSPLGYGGPALAPRPTISDARRRGLPWEREPSLETFGETLK
                     LAVGAPHEAFSSMRRSGGFGNPLGFMIVSYVVAQMLFLVEVFLFQLGWALLFQGSLRV
                     SWEVFFIGFGISAVVYLAVALFLATFGGFVVAGFYHLGLLMFGGANAGFEATYRVVAF
                     GTGSVYMLVPIPLVGPFFAVVMHFVVLIYGLMYAHQTTGGRAALAVFVPLFLATCCLC
                     PVLLMMMPAVPMLLSPLGR"
     gene            2535..>3233
                     /locus_tag="VFB80_17715"
     CDS             2535..>3233
                     /locus_tag="VFB80_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35674.1"
                     /translation="MPIEFHCSQCNQLLRVPDASAGKNARCPKCQTLMTVPAPALPNS
                     FAPPGSFASPSATPLAPAFSSSPPAKPAGAAPGNPFAGDALAKPGGNPFAGSINPYAA
                     PAGGYEYQSFAAPDAPIVNVQVPFEPIWNHAWSVWQANLGILVGVTAIIVGISYAIVI
                     PMAVVRFVLEQNNNRELGAVVGVLAQIANNLIQIYLGIGQAQISLKLARGQPAEVKDL
                     FAGGSRFLPVLGAVL"
BASE COUNT          494 a         1125 c         1026 g          588 t
ORIGIN      
        1 gtggtcggac gtgactacga ccagcgtgcg gttcagctcg ccggcgtcct ccaggatttt
       61 gagcgtgcgg ccaatctgct gatcgcccca ttcgacttcg atcgcatagt cggccaggtc
      121 gcggcggacg atatcggtgt cgggcaggaa cgccgggacc ttcacgtcgg ccagcttctt
      181 gcccagccgc acgccggagt cgaattcgta cgcgcggtgc ggctcggtaa agcccatcca
      241 gaagcagaac ggctgctcct tgggtcgctg tttgaggaac tcggcaaaat tgcccggata
      301 gtcgttgcgg ccgatgcccg aggccgggac ctcctgcgtc aggccgttaa agcccggccc
      361 ggcgggattg cgggtccgct ttgcctgcgt ctgaaaatcg cccggccccc agcccttgcc
      421 ggtcaggccg acgaaatagc cggccttctc cagcaggtcg ggatagacct cgaaacccgc
      481 gggaaacagg ccattgtgcg agacggcttc cttcagctgc caggtgttgc ggccggtcag
      541 gatcgaggcc cggcaggggc tgcacttggg gttcgaggtg aaggcgttct tgaagatcac
      601 cccttcgcgg gcgatgcggt cgaagtgcgg cgtcttgatc cagtcgcagc cgtaggcccc
      661 ggcgtgcgtg ctgccgttcc agtcgtcaaa gatgatgaag aggatgttgg ggcgggcgtc
      721 ggcggctggg gccgtggccg cgctcgccag cgccaggcag gcgatcattg agagccggtt
      781 aaccatgcga tgctcctgaa gagcggtcat tcggatttct ggtttcggat ttgtttgtga
      841 tttggtgctt ggtgcttggt cattccattc atagctaccg ggcaagtgta cttgaattaa
      901 gatacggatt cagcaggcag cgtcttcagc ttggcggcac gcagttccca tgcccatcga
      961 attctcgtgc cccgcctgcc atcagctggt ccgcacgccc gacgcggccg cgggcaagaa
     1021 gggcaagtgc cccctgtgcg gcgcggtcgt gcagattccc gcgccggccg ccgcgccggt
     1081 gaagccggcc ccagccaaac cagcgcctcc caaaccagcc gcccccaagc cagcccccaa
     1141 atcagcacca gtcaagcccg cgccggttcc ggttcctgag cccgagccgc cagcgcccga
     1201 gcccgccccg gacacgattg agtttgcctg cgtctggtgc aacgagctgg ttcgcacgcc
     1261 ggcctcggcc gcgggcaagc gcggcaagtg cccgcactgc cagcggatcg tcaaaattcc
     1321 caccgccgag cgccggctag tcaaagagcg cgacccgctg ctctccgaag agctgccgcc
     1381 gatggagaag gtcccgcacg gagcagcgcc caaggcagcc gcgcccaagc ccaagccggc
     1441 ggccaagccc agcacgccca gcgtgccagc cgccaggccg agcgtcccgc cgccgcccga
     1501 tctcctcgtt cccgtacagc ccgagccggc caaacccgct gcgctccagc ccgccaaacc
     1561 aaaaccgccg ccgccggagg tcgcggctgc cgcgggcctg gccgagatca aggtgccgga
     1621 tgtgacgccg atcaccgatc cgcagccgcc gccccccgat ccgttcctcg gtctgacgcc
     1681 gctgccttcc gccgccgagc cggaagggct gacgccgctg ctggacctgc ccaaggacag
     1741 ctttcagacg ctgacgccgc tggggccgga caacctcacg gtgctgacgc cactgccgga
     1801 cgatccgctc gatggcctgg aggccagcgg ctcgcccctg ggctacggcg gcccggcgct
     1861 cgcaccgcgg ccgacgatca gcgacgcccg ccgccgcggt ctgccctggg agcgcgagcc
     1921 ctcgctggag acctttggcg agacgctgaa gctggcggtc ggagccccgc acgaggcgtt
     1981 cagctcgatg cgccgcagcg gcggcttcgg caatccgctg gggttcatga tcgtgtcgta
     2041 cgtcgtcgcc cagatgctgt tcctggtcga ggtcttcctc tttcaactcg gctgggcgct
     2101 cttgtttcaa ggcagcctgc gagtttcgtg ggaggtgttc ttcatcggct tcggcatttc
     2161 cgcggtggtc tatctcgcgg tggccctgtt tcttgcgacg tttggcgggt tcgtggtcgc
     2221 cggcttttat cacctgggcc tcttgatgtt cggcggagcg aacgccggct tcgaagcaac
     2281 ctatcgggta gtcgcattcg gcaccggctc ggtatatatg ttggtgccga ttccgctcgt
     2341 ggggccgttc ttcgccgtgg tgatgcactt cgtcgtgctg atctacggac tgatgtacgc
     2401 ccaccagacg accggcggcc gggcggccct ggcggtgttt gtgccgctgt tcctggcgac
     2461 ctgctgcctg tgccccgtgc tgctgatgat gatgcccgcc gtgccgatgc tcctgtcccc
     2521 cctggggcgc taacatgccg atcgaattcc actgctcgca gtgcaatcag ctgctccgcg
     2581 tgcccgacgc cagcgcgggc aaaaacgccc gctgcccgaa gtgccagacg ctgatgacag
     2641 tgcccgcgcc tgcactgcca aactccttcg caccgccggg ttccttcgcc tcgccatccg
     2701 ccactccact cgcgccagcc ttttcgtcat ccccgcctgc taagcccgcc ggcgccgcac
     2761 cgggcaatcc ctttgccggc gacgccctgg ccaagccggg aggcaacccg ttcgccggga
     2821 gcatcaaccc ctatgccgcg cccgccggcg gctacgagta tcaatcgttc gcagcgcccg
     2881 atgctcccat cgtcaatgtc caggtgccct tcgagccgat ctggaatcac gcctggagcg
     2941 tctggcaggc gaacttgggg atcctcgtcg gcgtcacggc catcatcgtt ggcatctcct
     3001 atgccatcgt gatccccatg gccgtcgtgc gcttcgtgct ggaacaaaac aacaaccgcg
     3061 aactgggcgc agtcgtgggc gtcctggccc agatcgccaa taacctgatc cagatctatt
     3121 tgggaatcgg gcaggctcag atctcgctca aactcgcgcg cggccaaccg gcagaagtca
     3181 aggacctgtt tgccggtggg agtcgttttc tgcccgtgct gggggccgtg ctg
//
LOCUS       DASRJB010000667         5208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1606775, whole genome shotgun sequence.
ACCESSION   DASRJB010000667 DASRJB010000000
VERSION     DASRJB010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606775"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFB80_17720"
     CDS             <1..378
                     /locus_tag="VFB80_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35675.1"
                     /translation="HFVYRYGVLYLTTRENVKDWRDPTGIAAIRPPAGSPLAQAWEEP
                     SQIEAINMPLDKALQLLTQRLAIGVDLSGLPPESRVMPVTKNLRDLPLRHVLGLILDN
                     LNLRAEARGGDTIAILPPKPSIP"
     gene            1237..2952
                     /locus_tag="VFB80_17725"
     CDS             1237..2952
                     /locus_tag="VFB80_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZN35676.1"
                     /translation="MINVRSIPHIYRNVKRWTEIVAVLSKYGLADWLAQTNIEFAKDQ
                     LKHRDGEVLARLTREARIRLTLAELGPTFIKLGQLLSTRADITGKELAEELKLLQAST
                     PADPPETVRKVVETELGMPLDELFAEFDLTPIASASIGQVHRARLRSGERVVVKVQHA
                     GIESKVNEDLEVLAGLAQLAEQFPEFKPYRPTANVAEMGRTLRRELDYGREERNLHQF
                     AELFKNDPTICIPKAHTELSTPRVLTMDMIDGIPLAQPGLLEAAGLDKEEVARRGANL
                     YLQMIFTHGFFHADPHPGNIMLLPGNVIGLIDFGMVGRIDERLRENIEDMLMAIVQHD
                     VPLLVRVVKQIGQPPPQLDEAGLANDVADFVGHYSTQALDHLDVGSALVDMTQIMRRY
                     GIMLPPQVATLIKTLVTLEGTGRLLSPRFSLMEVMQPFQRGMILRRLSPVRQARKLRR
                     LYMEVEQLVEVLPQRVLQILEQIQSGKFDVHLDHRGLGPSVNRLVLGLLASALFVGSS
                     FLLSYKVPPLLFFRNDPATYTNWFGLHNISLLGLAGCTLSILIGLRLLWAIGKSGHLD
                     QKVSK"
     gene            3073..3804
                     /locus_tag="VFB80_17730"
     CDS             3073..3804
                     /locus_tag="VFB80_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35677.1"
                     /translation="MLVYRLAPNQTVRPEEMAVALQQRLVASGFSDASVTAHANGVRI
                     ELPGSGRADVSPVKTLLTKPGQLEFLICAERGSDDKVIGAAEDVPNGEESAEKGYRWI
                     RLDAKRVEPDSKMVVRKGRAGREEVLMLVDREFSITGADFREAHATVEGGVPCIAGAL
                     SAEGGHKMGYVTANNLKKRLGIVLDGELLSAPIIQSKITDRFQLTGRFSQQEVDTIVA
                     MLRSGTLPGRLEAEPASEEYVEPKK"
     gene            3851..4294
                     /locus_tag="VFB80_17735"
     CDS             3851..4294
                     /locus_tag="VFB80_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35678.1"
                     /translation="MSTAHSTSMWQFSTRTLFILTAAVAGLAWVFFAPPQWVGLMVLW
                     LLYWLLPAAALAGIVYHRGAWQAFFIGTAPWAVGVAIFLWIWAIDDGWRLVRDGLQLI
                     DEGTEELIQIKLMLGFPLFMAACSGALTVAIWAWAAGVRQRQPRS"
     gene            complement(4301..4954)
                     /locus_tag="VFB80_17740"
     CDS             complement(4301..4954)
                     /locus_tag="VFB80_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN35679.1"
                     /translation="MFDAVGTLIYPDPPVAVVYRGVARRFGLELSAAEIHDRFAAAFA
                     HEFAAAGLERPPTSEASERARWQRVVAAVLHELSAAAGEPFDLLWEHFARADSWRTFE
                     DVAPTLAELARRGVRLGIASNFDGRLHGIVQATPPLASCEQVFVSSEIGFSKPDRRFF
                     QSVQRQLNVSPDEILLVGDDVQNDQQGALAAGWRTVLVRRGAPASGANISALSELLS"
BASE COUNT          934 a         1654 c         1734 g          886 t
ORIGIN      
        1 cattttgtat atcgctacgg cgtgctttac ctgaccacgc gcgagaatgt gaaagattgg
       61 cgcgatccga ccggcatcgc cgcgatccgc ccgcccgctg gcagcccgct ggcccaggca
      121 tgggaggaac ccagccaaat cgaagcgatc aacatgccgc tcgacaaggc gctacaactg
      181 ctgacgcagc gcctggcgat cggcgttgac ctgagcggat tgccgcccga gagccgcgtc
      241 atgcccgtga cgaagaacct gagagacttg ccgctacgac acgtcttggg tctgatcctc
      301 gacaacctca acctccgcgc cgaagcccgc ggcggcgata cgatcgccat cctgccgccg
      361 aagccttcga ttccctgatc gtcgcagttc ccgcagaagg cgcggccccc tggttgggcg
      421 agtgcgatcg cgcctttttt gcactttctt gtcgcaactc ctgaggtgcc ataagcttgc
      481 ggcaaaggcg tccgcaggcg gttaagtact acttattagg gttagagaac cgcattcgcc
      541 gggcgcccta gcggcgctct tcaatccagg aacgacgtct aattgcaggc ccggagggcc
      601 gaccttcgtt agccctgggc gctgcggcgc agccgcgagc ccagggtcgg cgtccaaccg
      661 tcgaccggag ccccaacggg gcgcgatcaa cgcgccgggc cgggcgattt cgcccctgcg
      721 gggctgcgcg gcatcgtcat cgcattccgt gggccgcgcc cacggctgac gaaggccggc
      781 ccgttgggcc gcaccgatgg ttcatcgaca accgtcacca aggcccggag ggccgatagg
      841 tcgttcgacc atcgagcaaa tatcaccaca cgtggacaga attatccaaa tccgccggca
      901 catcgcaagc aacttgtaca cattttgttc aattcatgcc aacttgcgtc gaatggcctc
      961 gccacaactt caatgaccgc acccacttcc gccgccgcgc gtcgccgtaa gtttttttcg
     1021 cggccgctgg cggtttggac tagtgtggtc cattccggcg cagcccaaaa gccgcgaccc
     1081 ttggtcgcgc tccacgagca cagcgcgacc actggtcgcg gctttggggc accgactgac
     1141 caccgaccga ccggcccacc ggtagaccgc gaccattggt cgcggctttg cgggaacagg
     1201 ctgcattcgc tacgcggtgt aaactagaat cgagccatga tcaacgtccg ctcgatcccg
     1261 catatctacc gcaacgtcaa gcgctggacc gagatcgtgg cggtgctctc gaagtacggg
     1321 ctggccgact ggctggcgca gacgaacatc gagttcgcca aggaccagct caagcaccgc
     1381 gacggcgagg tgctggcccg gctgacgcgg gaggcgcgga tccggctgac gctggcggag
     1441 ctcgggccga cgttcatcaa gctggggcag ctgctcagca cgcgagccga cataacgggc
     1501 aaggagctgg ccgaggagtt gaagctgctg caggcctcga cgcccgccga tccgccggag
     1561 acggtccgca aggtcgtcga gaccgagctg ggcatgccgc tcgacgagct gtttgccgag
     1621 ttcgacctga cgccgatcgc ctcggcctcg atcggccagg tgcaccgggc ccggctgaga
     1681 tcgggcgagc gcgtcgtggt gaaagtccag cacgcgggga tcgagtcgaa agtgaacgaa
     1741 gacctggagg tgctggccgg cctggcccag ctcgccgagc aattcccgga gttcaagccg
     1801 taccggccga ccgccaacgt cgccgagatg ggccgcacgc tccgccgcga gctcgactat
     1861 ggccgcgaag aacgcaacct gcaccagttt gccgagctgt tcaaaaacga cccgacgatc
     1921 tgcatcccca aggcgcacac cgagctttcg acgccccggg tcctgacgat ggacatgatc
     1981 gacggcatcc cgctcgcgca gccgggattg ctcgaggcgg cgggcctcga caaggaagag
     2041 gtcgcccgcc gcggggccaa cctctacttg cagatgatct tcacgcacgg cttcttccac
     2101 gccgatccgc accccggcaa catcatgctg ctgccgggca acgtgatcgg cctgatcgac
     2161 ttcggcatgg ttgggcggat cgacgagcgg ctgcgggaga acatcgaaga catgctgatg
     2221 gcgatcgtgc agcacgacgt gccgctgctc gtgcgggtcg tcaagcagat cgggcagccg
     2281 ccgccgcagc tcgacgaggc ggggctggcc aacgacgtgg ccgatttcgt gggacactat
     2341 tcgacgcagg ccctggacca cctcgacgtc ggctcggccc tggtcgatat gacgcagatc
     2401 atgcggcggt acggcatcat gctgccgccg caggtggcga cgctcatcaa aacgctcgtc
     2461 acgctggaag gcaccggccg gctcctcagc ccgcggttca gcctgatgga ggtgatgcag
     2521 ccgtttcagc gggggatgat tctgcggcga ctctcgccgg tgcggcaggc ccgcaagctg
     2581 cggcggctgt acatggaggt cgagcagctg gtggaggtgc tgccgcagcg ggtgctgcag
     2641 attctggagc agatccagtc ggggaaattc gacgtccacc tcgaccaccg gggcctcggg
     2701 ccgagcgtca accggctggt gctcggcctg ctggccagtg cgctgttcgt cggctcgtcg
     2761 tttttgctga gctacaaggt cccgccgctg ttattttttc gcaatgatcc ggcgacttat
     2821 acgaattggt ttgggctgca caacatttcg ctcctcggcc tcgccggctg cacgctgagc
     2881 atcctgatcg gcctgcggct gttgtgggcg atcggcaaga gcggccacct cgaccaaaaa
     2941 gtttccaagt gacctccgtg ggcgggcctc cggccggcct agacaaagat gaatcttcgc
     3001 tgctggctgc tcattgtctt ggtattgctc gccggctgca gcaagccgac cgtcgccaaa
     3061 agcggcggaa ccgtgctcgt ctatcgcctg gcgccgaatc agacggtccg ccctgaggaa
     3121 atggcggtgg cccttcagca gcgcctggtg gccagcggtt tttccgacgc aagcgtgaca
     3181 gcgcatgcca atggcgtacg gatcgaattg ccgggaagcg gccgagccga cgtcagcccg
     3241 gtgaaaacgc tgctcacgaa gcccgggcaa ttggagttct tgatctgcgc cgagcggggc
     3301 agtgatgaca aggtgatagg ggcagcggag gacgtcccga acggtgagga gtcggccgaa
     3361 aagggctatc gctggattcg cctcgatgcc aagcgcgttg agccggacag caagatggtg
     3421 gtccgaaagg gtcgggccgg gcgtgaggag gttctcatgc tggtggaccg cgagttcagc
     3481 atcaccggcg ccgacttcag ggaagctcac gcgactgtgg aagggggcgt tccgtgcatt
     3541 gccggcgccc tttccgccga aggcggacac aagatgggct atgtcaccgc gaacaacctg
     3601 aagaaacggt tgggcatagt cttggatggg gagctgctca gcgcgccgat cattcagtcc
     3661 aagatcacgg accggtttca gctcacaggt agattctcgc agcaagaagt cgatacgatc
     3721 gtggcgatgc tccgcagcgg cacgctgccc ggacggctcg aagcggaacc ggcgtcggaa
     3781 gagtacgtcg agccgaagaa gtagcgtggg ctgtgcccac cagtggcaaa gggtgccatg
     3841 gaggcagatg gtgagcacgg cccattcaac cagcatgtgg caattcagca cgcggacgct
     3901 gttcatcctc acggccgcgg tcgcggggtt ggcgtgggtc ttctttgcgc cgccgcagtg
     3961 ggtggggctg atggtgctct ggctgctgta ttggctgctg ccggcggcgg cgctggcggg
     4021 aatcgtctat caccgcggag cgtggcaggc gttcttcatc ggcactgcgc cgtgggccgt
     4081 cggcgtggcg atctttctct ggatctgggc catcgacgac ggctggagac tggtccgcga
     4141 tgggttgcag ctgatcgatg agggcacgga ggagctgatt caaatcaagc tcatgctggg
     4201 ctttcccttg ttcatggcgg cgtgcagcgg agccctgacg gtggcgatct gggcctgggc
     4261 agccggcgtt cggcagcggc agccgcgtag ctgagccgtg tcaggaaagc aattcgctga
     4321 gcgccgagat gttcgcgcca gacgcaggtg cgccgcggcg cacgagcacg gtccgccagc
     4381 cggccgccag tgctccttgc tggtcattct gcacgtcgtc gccgaccagc aggatctcgt
     4441 ccgggctgac gttcagctgg cgctgcaccg attgaaagaa gcggcggtcc ggcttgctga
     4501 agccgatctc ggacgagacg aagacctgct cgcacgaggc gagcggcggt gtggcctgga
     4561 cgatgccgtg cagccggccg tcgaagttcg acgcgatgcc caggcgcact ccgcggcggg
     4621 ccagctcggc cagcgtgggc gcgacgtctt cgaacgtgcg ccacgaatcg gcccgggcga
     4681 agtgctccca caaaagatcg aacggctctc cggcggcagc ggacagctcg tgcagtacgg
     4741 cggcgacgac tcgctgccag cgagcccgct cgctggcttc gctggtcggc ggccgttcca
     4801 atcccgcggc ggcgaattcg tgtgcgaatg ccgctgcgaa gcgatcgtga atctcggcgg
     4861 cgctcaactc caggccaaaa cgccgcgcga ccccgcgata gactaccgct accggcggat
     4921 ctggatagat cagcgttccg acggcgtcga acaagatcca gcggacgctg tcgagcggca
     4981 gtctcgatgt agccgaattt gccaaaattc ggtgactcca gcggcaccga attctggcga
     5041 actcggctac gagttacagc agctcttcga tttccaggcc ggcgaggcga tcgaccagct
     5101 cggcggccgc cggccggcgg agcggctcct tggagagcat tcgccgcacg agctgccccg
     5161 agtcgcgcga caccgtcgga acaaaatcgc gcacgtccgg cggcagct
//
LOCUS       DASRJB010000668         5517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3017804, whole genome shotgun sequence.
ACCESSION   DASRJB010000668 DASRJB010000000
VERSION     DASRJB010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017804"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="VFB80_17745"
     CDS             <1..1986
                     /locus_tag="VFB80_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35680.1"
                     /translation="VSELAYDAWAKAPEVTLQGWGRIEGSILWKDKPGAGEPVSLIVH
                     RDDYGYPGMVAQYAKSKADADGKFVFDRILPGHVQLSRPLPANGGGETILPGMFIHLK
                     VAAGQPTPAVIGGQGRQVTGKLKGRDSWNDVTLHFHPNAPHIGFPGDDEQWKAFSELK
                     AGPLGLLLFRDKIKPAADGTISIDNVLPGQYQLFVSAPGVQNYAGYLQFRVEPEVPGA
                     KPQPQNLGEIQVKAAAQAAAKPVEKPVTAQPAERNTASDKRVSINGKAVDAETGKPVA
                     PLIVQAGKFDPADPTKVTWGYSEQRSSSTTGAFSTSVTWGQGWTARVLADGYLPEPVI
                     TSAPPEGTDELTVTLKLRRGRLVRGRVLDHAGKPVKGAAIFAIGPTGVNLAGGKAYSS
                     WGEADKTAKSVLTDDAGRFEIPAGDAKSLAVSGAAIDAWPGAIASEGETTIKLPEAAR
                     VEVELNIDGAEDDGEFFYQLLGHRMEGFEKLQSTRQFPLKNGGKLTLPALPPGKYQFC
                     RQVMNRLADFGAGAMLDRQFIELKPGETKAIRYVRDKGVRVRGKLTWPAGTVLAGTIV
                     SVRSPTKEKDPFDGHEWQTTFASQVAVADGTFLTERLLPGKYLLVADAYTPLTPEQKV
                     RSGIIGPSLSAEMPITVPESGEFTAPDLPLKPIVRRP"
     gene            complement(1961..2692)
                     /locus_tag="VFB80_17750"
     CDS             complement(1961..2692)
                     /locus_tag="VFB80_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35681.1"
                     /translation="MNSTWFLHLLPILCIWLAGGLIAIVRWHRHPGVCALVVLSIVTM
                     IAATSATHIAVQYFTRSNSPVLVQQYLAMTGLVNMLLRTIAWGAILTAVFGWRPAPIG
                     VARPMLQFSIRGLLTLTLAVALLCAAGRAIVGWLGGVSQYWISIVDDLPMAICWLCGV
                     WIAIRRWPAHPPVSAIALAAIALSAASFLAFHLVWMSNLNSGTIYLVQWASLLSGIIA
                     PIVWALLLAAALGWRADATAAAQSA"
     gene            complement(2730..3404)
                     /locus_tag="VFB80_17755"
     CDS             complement(2730..3404)
                     /locus_tag="VFB80_17755"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN35682.1"
                     /translation="MPVNTLESAPDRSETSRVEEVYERVLLQIVRGELAAGTALRSTQ
                     VARQLGISRTPVVQALQRLAADGIVTLEMNKRAVVRPGAEKWLVEIHELRELLEPAAA
                     ARAAERITEDELSRLDDLAAAARPHSQSDWEAAAQAFDFALHLAIADHAGNFALGEAI
                     RKIWKFKRISYLAAPEPAATLERGYAEHLALLAALHARDGETARAAMHFHLRSAATLR
                     GAKNIV"
     gene            3496..4779
                     /locus_tag="VFB80_17760"
     CDS             3496..4779
                     /locus_tag="VFB80_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35683.1"
                     /translation="MGVAASHSLRAGMVGMGMIFDETYRPFFEQAHAAGVYDPRFCVC
                     GVELAAVASRTGSRAEAYRQAAAGRIANFVSFKEPDSIGRLLKHGVDFVCVATPDDRH
                     FAASKAAILAGKHVLIEKPSVLSLAELDELDRLARQKDVLAKVVYHKLLDPDHKKLRT
                     LVADGELKHVNTGYCTLLEPKSISGGQFAEWIAGRNPGTYVAVHYIKLIDFTFGGRLK
                     SVACTGQRGLVGPASGPTWDSTQLRMIYEYSDGREAAFDIHTSWVTPDNFPGYVDQEV
                     QFRFDNGVWNAHSRRRGVECTVEERTPFLRKININNHYNGQFLEPWGERSQRGYGVEV
                     LERFVREVATVEFGGPPAERPTRLAEARKLAYNDISADRQVVAAVQAMEAILAKHAAG
                     TPNCIVEVDHRLGGLVLLSPGKSEPEVLYGGRVDA"
     gene            4865..>5517
                     /locus_tag="VFB80_17765"
     CDS             4865..>5517
                     /locus_tag="VFB80_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35684.1"
                     /translation="MTANVLETPTPIQHAGEDTSNDIRRRLAEVEPHCIRTFTPSLAV
                     LAKSAGSYHWTPEGRKLADFTSGVLVMNLGHNPTRWWRRVKDYLGLHDLKSAGEFLPA
                     VTLNAYNAVTEVEALATERLVALMQQQPGGERCEQVLWAASGSEAIQKALWAALDRRP
                     GENILLSTRHGFHGKKGLAGAVTGCEADKERDPRVEFIGFPRNECLNVARRRQPIDLA
                     "
BASE COUNT         1050 a         1753 c         1851 g          863 t
ORIGIN      
        1 gttagtgaat tagcgtacga cgcctgggcg aaagcgccgg aagtgacgct gcaaggttgg
       61 ggccgcatcg aaggaagcat cttgtggaag gacaagcccg gcgccggcga acccgtgagc
      121 ctgatcgtcc atcgcgacga ctacggttat ccgggcatgg tcgcgcagta cgccaagagc
      181 aaggcggacg ccgacggcaa gttcgtgttc gaccggattt tgcccggcca cgtgcagctt
      241 tcgcggccgc tgccggccaa tggcggcggc gagacgattt tacccggcat gttcattcat
      301 ctgaaggtcg ccgcgggcca gccgacgccg gccgtcatcg gggggcaggg gcggcaagtg
      361 accggcaaac tgaagggacg cgattcctgg aatgatgtca cgctgcactt tcatcccaac
      421 gcgccgcaca tcggcttccc cggcgacgac gagcagtgga aggcgttctc agagctcaag
      481 gccggcccgc tgggcctgct gctgttccgc gacaagatca agccggctgc cgacggcacg
      541 atttcgatcg acaacgtgct gccgggccag taccagctct tcgtctccgc gcccggagtg
      601 cagaattacg ccggctattt gcagtttcga gtcgagccgg aagttcccgg cgcgaagccg
      661 cagccgcaga atttgggcga gattcaagta aaagcagcgg cccaggcggc cgcgaaaccg
      721 gtggagaagc cggtgaccgc gcagcccgcc gagagaaata ccgcgagcga caagcgcgtc
      781 tcgatcaatg gtaaggcggt cgatgcggaa accggcaagc cggtcgcgcc gctgattgtt
      841 caagcgggca agttcgaccc ggcggatccg acgaaagtga cctggggcta cagcgagcag
      901 cgctccagct ccacgaccgg cgcgttctcg acctcggtta cttggggcca gggctggacg
      961 gcgcgggttt tggccgacgg ctatctgccc gagccggtga ttacgtctgc gccgccggag
     1021 ggcaccgacg agctcacggt cacgctcaaa ctgcgccgcg ggcggctggt ccgcggccgc
     1081 gtgctcgatc acgctggcaa gccggtgaag ggcgcggcga tattcgcaat cggcccaacg
     1141 ggcgtcaacc tggcaggcgg caaggcgtat tcgagctggg gcgaagcaga caagacggcg
     1201 aagtcggtgc tgaccgacga cgccgggcgg ttcgagattc cggccggcga cgccaagtcg
     1261 ctggctgtct cgggcgcggc gatcgacgcc tggccggggg caatcgccag cgagggcgag
     1321 acgacgatca agctgcccga ggccgcgcga gtcgaagtcg agctgaacat cgacggcgcg
     1381 gaagacgacg gcgaattctt ctatcagctg ctcggccatc ggatggaggg tttcgagaag
     1441 ctccaatcga cccggcagtt tccgctgaag aacggcggca agctgacgct gcccgcgctg
     1501 ccgcccggca aataccagtt ttgccggcag gtgatgaacc gcctggccga cttcggcgcg
     1561 ggcgcgatgc tcgaccggca gttcatcgag ctgaagccgg gcgagacgaa ggccatccgc
     1621 tacgtccgcg acaagggcgt gcgggtccgc ggcaagctca cctggccggc agggacggtg
     1681 ctggccggaa cgatcgtctc ggtccgcagc ccaacgaaag aaaaggaccc ctttgacggc
     1741 cacgaatggc agacgacctt tgcgtcgcaa gtcgccgtcg ccgacggcac gttcctgacc
     1801 gagcggctgc tgcccggcaa gtatctgctg gttgccgacg cctacacgcc cctcacgccg
     1861 gagcagaaag tccgctcggg gatcatcggg ccgtcactga gcgcggaaat gccgatcacg
     1921 gttcccgaat cgggcgagtt tacggctccc gacctgccgc tcaagccgat tgtgcggcgg
     1981 ccgtagcatc ggctcgccag ccgagggccg cggcgagcag gagcgcccag acgatcggtg
     2041 caatgatgcc cgagagcaga ctggcccatt gcaccaggta aatcgtgccg ctgttcagat
     2101 tcgacatcca gaccaggtga aaggccagaa agctggcggc gctgagcgcg atggccgcca
     2161 gcgcaatggc ggaaaccggc ggatgcgccg gccagcggcg gatggcaatc cagacgccac
     2221 acagccagca gatggccatc ggcagatcgt ccacgatcga aatccaatac tgcgaaacgc
     2281 cgccgagcca accgacgatg gcccgccccg cggcacagag cagcgcgacg gccagcgtca
     2341 gggtcaacag gccgcgaatg ctgaattgca gcatcggtcg agcgactccg atcggagccg
     2401 ggcgccagcc gaaaacggcc gtcaggatcg ccccccacgc gatcgtgcgg agcagcatgt
     2461 tcaccagccc ggtcatcgcc agatattgct gcaccaagac gggtgagttc gatcgcgtaa
     2521 agtactgcac ggcgatgtgc gtagcgctgg tggcggcgat catcgtgacg atcgaaagca
     2581 ccaccagcgc acagacgccc ggatgcctat gccagcggac gatggcgatg aggcctccgg
     2641 cgagccagat gcacagaatc ggcagcaagt ggagaaacca ggtggaattc acaggcgata
     2701 atcttagccg acgcggggaa agaatgccgc taaacgatat tcttcgcgcc gcgcagcgtc
     2761 gctgccgagc ggaggtgaaa gtgcatcgcc gcgcgggctg tttcaccgtc gcgggcgtgc
     2821 agtgcggcga gcagcgccag atgctcggca tagccgcgct ccagcgtcgc ggcaggctcg
     2881 ggcgcggcga gataggaaat ccgcttgaat ttccaaatct tgcggatcgc ctcgcccagc
     2941 gcaaagttgc ccgcgtgatc ggcgatcgcc aggtggagcg cgaaatcgaa cgcctgggcc
     3001 gccgcttccc aatccgactg cgagtgcggc cgggcggcag cggccagatc gtcgagccgc
     3061 gacagttcgt cctccgtgat ccgctccgcg gcccgcgcgg cggccgccgg ctccaagagc
     3121 tcccgcaact catgaatctc cacgagccat ttttccgcac ccggccgcac cacggcccgc
     3181 ttattcattt caagcgtgac gatgccgtct gccgcgagcc gctgcagcgc ctgcacgact
     3241 ggcgtgcggc tgatgccgag ttgcctggcg acttgcgtac tccgcagggc ggttccggcg
     3301 gccagttcgc cgcggacgat ttgcagcagg acccgctcat agacctcttc gacccgcgac
     3361 gtttcgctgc ggtcgggggc ggattcgagc gtattgacgg gcattggaat ggcggctaaa
     3421 gtgaattcac attgatcgct actaattggc attataacca tttgcgacga atacgctaga
     3481 tatatggatt cacatatggg cgttgcagca tcccattccc tgcgggccgg catggtcggc
     3541 atggggatga tctttgacga gacttaccgc ccgttcttcg agcaggcgca tgcggccggg
     3601 gtctacgatc cgcggttctg cgtctgtggc gtcgagctcg cggccgttgc cagccggacc
     3661 ggcagccggg cggaagccta tcgccaggcg gcggctggtc gcatcgcgaa ctttgtgagc
     3721 ttcaaggagc cggactcgat cgggcggttg ctgaagcacg gcgtcgattt tgtctgcgtc
     3781 gcgacgccgg acgaccggca cttcgcggca tcgaaggcgg cgatcctggc ggggaagcac
     3841 gtactgatcg agaagccctc ggtgctctcg ctcgcagagt tggacgagct cgaccggctc
     3901 gcgcggcaga aggacgtgct ggcgaaagtc gtctatcaca agctgctcga cccggaccac
     3961 aagaagctgc ggacgctggt ggccgacggc gagctgaagc acgtcaacac cggctactgc
     4021 accctgctgg agcccaagag catcagcggc gggcagttcg ccgagtggat cgccggccgc
     4081 aaccccggaa cgtacgtcgc cgtccactac atcaagctga tcgacttcac attcggcggc
     4141 cggctgaaga gcgtcgcctg caccgggcag cggggcctcg tcgggccggc tagcgggcca
     4201 acgtgggact cgacgcagtt gcgaatgatc tatgagtatt ccgatggccg cgaggcggcg
     4261 ttcgacattc acacgagctg ggtcacgccc gacaattttc ccggctacgt cgatcaggag
     4321 gtgcaattcc gcttcgacaa cggcgtgtgg aatgcccaca gccggcggcg cggcgtcgag
     4381 tgcacggtcg aggagcggac gccgttcctg cgaaagatca acatcaacaa tcactacaac
     4441 ggccagttcc tggagccctg gggcgagcgt tcgcagcggg gatatggcgt ggaagtgctc
     4501 gagcggttcg tccgcgaggt ggcgaccgtc gaattcggcg gcccgcccgc cgagcggccg
     4561 acgcgtctgg ccgaggcgcg gaagctcgcc tacaacgaca tttccgccga ccgccaggtc
     4621 gttgccgccg tgcaggcgat ggaggcgatc ctcgccaaac acgccgccgg cacccccaac
     4681 tgcatcgtcg aggtcgatca cagactaggc ggcctcgtcc tgctctcgcc cggcaagtcg
     4741 gagccggagg tgctttatgg tggtcgcgtc gatgcgtgat gtcatttgtc aattgtcatt
     4801 cgtcatttgc aggtttctca acaagtgacc agtgacaagt gacaacagaa cgaggattca
     4861 tctgatgacc gcaaacgttt tggagactcc caccccgatc caacacgccg gcgaggatac
     4921 ctcgaacgac atccgccgcc gactcgcgga agttgagccg cactgcatcc gcacatttac
     4981 gccgagcctg gccgtactgg ccaagagcgc cggctcgtat cactggacgc ccgagggccg
     5041 caagctggcc gatttcacgt cgggcgtctt ggtgatgaac ctgggccaca atccgacgcg
     5101 ctggtggcgg cgggtgaagg attacctggg gctgcacgac ctgaaaagcg ccggtgagtt
     5161 cctgccggca gtcacgctca acgcctacaa cgccgtgacc gaggtcgagg cgctggcgac
     5221 cgaacgcctc gtggccctga tgcagcagca gccgggcggg gagcgctgcg agcaagtgct
     5281 gtgggccgcc agcggcagcg aggcgatcca gaaggcgctc tgggcggcgc ttgatcgccg
     5341 ccctggtgaa aacatcctcc tcagcacccg ccacggcttt cacggcaaga aggggctggc
     5401 gggcgcggtg accggctgcg aagcggacaa agagcgcgac ccgcgggtcg aattcatcgg
     5461 cttcccgcgc aatgagtgcc tgaacgtcgc ccgccgccga cagccgatcg acctcgc
//
LOCUS       DASRJB010000669         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3860664, whole genome shotgun sequence.
ACCESSION   DASRJB010000669 DASRJB010000000
VERSION     DASRJB010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3860664"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..>2112
                     /gene="ggt"
                     /locus_tag="VFB80_17770"
     CDS             589..>2112
                     /gene="ggt"
                     /locus_tag="VFB80_17770"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN35685.1"
                     /translation="MTTGNAELLSGYDRGGTHPNQSRSVVMAKHGIVATSHPLASQNG
                     LDVLKAGGNAADAAIAASATIAVVEPMSCGIGGDLFVIYWDNKTQKLYGLNASGRSPY
                     NLTRQVLAEKNLKQIPTEGPLSWSVPGCVSGWETLRARFGSQPLAKLLEPAIAAAEEG
                     CPVPEVIAGYWRGAETALKATPEAAQAFLINGERTPRLGEVIKLPQMAASLRLIAKDG
                     RDAFYKGEIAKKMVAYSESHGGYFSLKDFADHTDDWVEPVSTNYRGYDVWELPPNGQG
                     IAALEMLNVLERFDLKSLGHNSPEHIHLFIEAKKLAFADRAKFYSDPEFNRLPVTELI
                     SKEYGRKQAARITPRASADVPPGDPRLARGDTIYMCVVDKDRNCCSLIQSNYMGFGSK
                     HVPGDVGFCLQNRGALFALEENHLNRLEPHKRPFHTIIPAFVTKDGKPWLCFGVMGGD
                     MQPQGHVQILLNMIDFGMNVQAAGDAPRVSHAGSATPTGLPAEGSGTVSVEAGVSDEV
                     "
BASE COUNT          376 a          622 c          738 g          376 t
ORIGIN      
        1 gctggcttac catgtgcggc cggacctgat cgattgggcg gagcagatgg gcttgccgct
       61 tgcgccgctg cattcgtcca agggttttgg tcacgagatg atcttgcgcg ttcgccgtga
      121 tcgcgtctct acggaccatt gtactcagct cttcgcctgg ggcttggatc aagcctgaca
      181 caaactgtgt tgacgcctgc gaaggcggcg ttggggattc agcagccgac gatttccggc
      241 cggccggagg cccgccccac agtaggtccc gcgtgccgag cgggacctgg tcggcggatg
      301 gacgcatttg gcggagcaaa ccctgaccgg cgctggcagg tcgctgcgct cccccaaggt
      361 cccgtccggc agacgggacc tactggggcg cgtggagttt cgactcggcg ggcagtacat
      421 tcttggtgct gcttcttgag gagtcccgcc atggttttcc ctcgcttgcg cttcgggctc
      481 gtactccctc gctggcgcgt cgggttcgtg ttattggtta tggctatgaa ctcggatttg
      541 tatgggcagc cgctgaggac gccgattgtt caggaaaatg agacgccagt gaccacgggg
      601 aatgcggagt tgctgtcggg gtatgaccgc ggaggcacgc atccgaatca gagccgatcg
      661 gtggtgatgg cgaagcacgg catcgtggcg acgagtcacc cgctggcgtc gcaaaacggg
      721 ctggatgtgc tgaaggccgg cgggaatgcg gcggatgcgg cgattgcggc gagcgcgacg
      781 attgcggtcg tggagccgat gagctgcggg attggcggcg atttgtttgt catctattgg
      841 gataacaaga ctcagaagtt gtatgggctg aacgccagcg ggcggagccc ctacaacctc
      901 acgcggcagg tgttggccga gaagaatctc aagcagattc cgaccgaagg gccgctgagc
      961 tggtcggtgc ccggctgcgt cagcggctgg gagaccttgc gggcccggtt tggcagccag
     1021 ccgctggcga agctgctgga gccggcgatt gccgcagccg aggaaggctg cccggtgccg
     1081 gaagtgatcg ccgggtactg gcgcggcgcc gagacggcgc tcaaggccac gccggaagcg
     1141 gcccaggcgt ttctgattaa cggcgagcga acgccgcggc tgggcgaagt gataaagctg
     1201 ccgcagatgg ccgcgtcgct gcggctgatc gccaaggacg gccgcgacgc gttctataag
     1261 ggcgaaatcg ccaagaagat ggtggcctac agcgaatcgc acggcggcta cttctcgctc
     1321 aaagactttg ccgaccacac cgacgactgg gtcgaacccg tcagcacgaa ctatcgcggc
     1381 tacgacgtct gggagctgcc gcccaacggc caggggattg cggccctgga gatgctgaac
     1441 gtgctggagc ggttcgacct caagagcctg ggccacaatt cgcccgagca catccacctg
     1501 ttcatcgagg ccaagaagct ggccttcgcc gaccgggcga agttttattc ggacccggag
     1561 ttcaaccggc tgcccgtgac cgagctgatc tcgaaggaat acggccgcaa gcaggctgcg
     1621 cggattaccc cgcgggcgtc ggccgacgtg cccccgggcg atccgcggct ggcccgcggc
     1681 gatacgatct acatgtgcgt ggtcgataag gaccgcaact gctgctcgct gattcagagc
     1741 aactacatgg gatttggctc caagcacgtg ccgggcgacg tcggcttctg cctgcagaac
     1801 cgcggggcct tgttcgccct cgaagagaac cacctcaacc ggctggagcc gcacaagcgg
     1861 ccgttccaca cgatcatccc ggcgtttgtg acgaaggacg gtaagccctg gttatgcttc
     1921 ggcgtgatgg gcggcgacat gcagccgcag gggcacgtgc agattctgtt gaacatgatc
     1981 gactttggga tgaacgtgca ggcggccggc gatgcccccc gcgtgagcca cgccggcagc
     2041 gccacgccga cgggattgcc ggcagaaggg agcggcaccg tttcggtcga agcaggcgtg
     2101 agcgacgaag tc
//
LOCUS       DASRJB010000670         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1883772, whole genome shotgun sequence.
ACCESSION   DASRJB010000670 DASRJB010000000
VERSION     DASRJB010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883772"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFB80_17775"
     CDS             <1..453
                     /locus_tag="VFB80_17775"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN35686.1"
                     /translation="LSDVQTNIHLAGVRGATIVGNTLWQGYSHNIHLEECTAVVIGAN
                     MLERNPLYGYTSEGKDGVLLRRCRDCTIQGLHLHNVIGSPAGLILENCDRLHVTGCTI
                     LDCDNAGLLVKDCQRSTILGNVIRDDRDAATTAPIKVEGGAANTIQNP"
     gene            523..906
                     /gene="mgsA"
                     /locus_tag="VFB80_17780"
     CDS             523..906
                     /gene="mgsA"
                     /locus_tag="VFB80_17780"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HZN35687.1"
                     /translation="MLALIAHDQKKPALVEFVLRHRDILERFPLVATGSTGRLIAERT
                     GLKVECVAHGPDGGDLIIGARVALGQIRAVIFFRDPLTAQPHEPDVSALMRVCDVHRI
                     PLATNLGTADAIAAWLNDLALGTPA"
     gene            complement(1190..1843)
                     /locus_tag="VFB80_17785"
     CDS             complement(1190..1843)
                     /locus_tag="VFB80_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35688.1"
                     /translation="MAVRCAKCGEELLGAVNRCWKCGTTFAARPEIDGRPPVMLEMPP
                     AEAVASAAPLDAVILDDPPAEPAAIDALPADGDPPPAAASPPAPTPYYPPPQPPMATV
                     LVKPRLAQRATSTPADRVEALRRSNMAMGGTVGAFVLGVFALVISLFRFEAALIAVLG
                     LIMGIWGLQSQRRNWALTAMLLCCLSIGIGAYTGVRQLYVYIQKSRPIEMPDTSEQP"
     gene            1977..>2474
                     /locus_tag="VFB80_17790"
     CDS             1977..>2474
                     /locus_tag="VFB80_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HZN35689.1"
                     /translation="MHAWRSLVLFLFCLILAASTGAIAAPPDAPPPVEPKIAAASDEG
                     QQALAGFQIPAGLKGELWAAEPLLANPVAFCLDNRGRVFVCESFRQKRGIEDNRDHSR
                     WLDDDLAAQTVADRLAYIQKHLGERAADYTKQDDRIRLLEDTDGDGRADKASVFADRF
                     NQIVDG"
BASE COUNT          435 a          854 c          770 g          415 t
ORIGIN      
        1 ctctccgacg tgcagacgaa tattcacctc gccggcgtcc gcggcgcgac gatcgtcggc
       61 aacacgctct ggcaaggcta ctcccacaac attcatctcg aagaatgcac ggcggtcgtc
      121 atcggcgcga acatgctgga gcgcaacccg ctgtacggtt acacctccga aggcaaggac
      181 ggcgtcctgc tccgacgctg ccgcgactgc acgattcagg gcctgcacct gcacaacgtc
      241 atcggttcgc ccgccgggct gatcctcgag aactgcgacc ggctgcacgt caccggctgc
      301 acgatcctcg actgcgacaa cgccggcctg ctcgttaagg actgccagcg ttccaccatc
      361 ctcggcaacg tcatccgcga cgaccgggat gcagccacca ccgcccccat caaggtcgaa
      421 ggtggcgccg ccaataccat ccaaaacccg taggtcagtc ggtcggtcgg tcagtcagtt
      481 gccaaccgac caccgaccaa ccgaccaccg accaaccgac ctatgctcgc cctcatcgcc
      541 cacgaccaga aaaagccggc cctggtcgag ttcgtcctgc ggcatcgcga catcctggag
      601 cggtttccgc tcgtggcgac cggcagcacc ggccgcctga ttgccgaacg gacgggcctg
      661 aaggtcgagt gcgtggccca cgggccggac gggggcgatc tgatcatcgg ggcccgcgtc
      721 gccctgggtc agattcgtgc cgtgatcttc tttcgcgatc ctctgaccgc ccagccgcac
      781 gagccagacg tcagtgcctt gatgcgggtt tgcgacgtgc accgcatccc gctggctacc
      841 aacctgggca cggccgacgc catcgccgcc tggctgaacg acctcgccct ggggacaccc
      901 gcgtaaaact ccctcgctct ctaaccgtaa tgtgtcgccc cctggatagg tagcgcttgc
      961 agccgcctat gtcggcgtaa agtcatcttg ccgcgtaact taagtgccaa ttcgggaacc
     1021 tggcttaagt agttgttatt agcgtttgag agcggcgttt ttcttgtcac gctctgggcc
     1081 gctgctggcc aaaatggcag ccgcgttgcc aaacggaact caagagcaaa tagcgtacca
     1141 ttgtacacaa atccggacgc cggccacccg caccagggaa gtcctcgccc tatggctgct
     1201 cgctcgtgtc cggcatctcg atcgggcggc tcttctggat atagacgtag agctgccgca
     1261 cgccggtgta ggcgccgatg ccgatcgaca ggcagcagag gagcatcgcg gtgagggccc
     1321 agttgcggcg ctgcgactgg aggccccaga tgcccatgat cagccccagc acggcgatca
     1381 gcgccgcctc gaagcggaac agcgagatca ccagggcgaa tactcccaac acaaaggcgc
     1441 cgacggtgcc gcccattgcc atgttgctgc gccgcagggc ttcgacgcga tccgccggcg
     1501 tgctggtggc tcgctgcgct aaccgcggct tgacgagcac cgtggccatc ggcggctgcg
     1561 gcggtgggta atagggcgtc ggggcgggcg gtgaggcagc ggctggcggt gggtcgccgt
     1621 cggccggcag agcgtctatg gcagccggct cggccggtgg atcatcgaga attaccgcat
     1681 cgagcggcgc ggcgctggcg acagcctcgg cgggcggcat ttccagcatc accggcgggc
     1741 ggccgtcgat ctccgggcgg gcggcgaacg tcgtcccgca cttccagcag cggttgaccg
     1801 cgccgagcag ctcctcgccg cattttgcac accggacggc catgagcgat tcgggcgacc
     1861 ttgcgaaccc caggggagag aactaggctg atgagtcccg ccgtagacct gtctatctat
     1921 ccatgatcta cgccgccggc gcggcgtcaa tctgccctgg gggcggagct tcttccatgc
     1981 acgcttggcg ctcgctggtt cttttcctgt tttgcctgat tttggcggcc tcgacgggcg
     2041 cgattgctgc tccacccgac gctccgccgc ccgtagagcc gaagatcgct gccgcctcgg
     2101 atgaggggca gcaggccctg gccggcttcc agatacccgc cgggctgaaa ggcgagttgt
     2161 gggccgccga gccgctgctg gccaacccag tcgcgttctg cctggacaat cgcggccgcg
     2221 tcttcgtctg cgagagcttt cgccagaagc ggggcatcga agacaaccgc gaccacagcc
     2281 gctggctcga cgatgacctg gccgcccaga ccgtcgccga ccgcctggcc tacatccaaa
     2341 aacacctcgg cgaacgagcc gccgattaca cgaagcagga cgaccgcatc cgcctgctcg
     2401 aagacaccga cggcgatggc cgggccgaca aggcgtcggt ctttgctgac cgcttcaacc
     2461 agatcgtcga tggc
//
LOCUS       DASRJB010000671        17013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2452509, whole genome shotgun sequence.
ACCESSION   DASRJB010000671 DASRJB010000000
VERSION     DASRJB010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452509"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFB80_17795"
     CDS             <1..502
                     /locus_tag="VFB80_17795"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN35690.1"
                     /translation="GTMISAVSRVEADGSIVSDLKFERSLVTPRRQPEGGDPAAITPQ
                     GTASVNVRTTVRARPGEPVLVGGRQHGSGKDAAQTWLVLTASVAPRAAGEKPKAAAAA
                     GPEEELKIVQLKNASAPALATVLASVFQREFRIAVDERTNSLLLRGSPERLVIAVALI
                     ARLDES"
     gene            777..3287
                     /locus_tag="VFB80_17800"
     CDS             777..3287
                     /locus_tag="VFB80_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN35691.1"
                     /translation="MRSRLTAAPRLAALFAAAFLLTHANAADTPNKPKAPGLGDPGKL
                     AAIQIETGRLKDGLVTVSGRDAGQQIVVTGQYDSGQTRDFSRKCTYEVSPAGIVNVDS
                     TGLVTPVAEGEATVHVVAEPGIDGNLKVKVTNLVTDLPINFPNQITPVFTKYSCNGGG
                     CHGKSGGQNGFRLSLLGFEPKEDYEYLWKEGRGRRLFPAAPDQSLLLLKATARLPHGG
                     GQRIEFDSPAYRLLKRWIEQGMPYGKDTDPKVTHIEVLPAERLMEREGTQQIVVVAHY
                     SDGSSEDVTRTTQFDSNDTEMAEVSVSGLVTTSQLTGSVAVMARYQGHVGVFRATIPL
                     GVKTDNLPPGKNFVDEFVQKKLKSLGLPSSAICDDATFLRRVSVDLAGRLPTLEETEQ
                     FLADAAPNKRENLVNRLLDSTDYADYFANKWSAILRNKRRQDTEKLSTFSFHEWIRQS
                     LHENKPYDQFAREILTATGTASVNPPAGWYREVKDASAQLEDTAQLFLGLRIQCAKCH
                     HHPFEKWSQQDYYGFSAFFSQVGRKRGDTQDSEKIFHRRGVAQAQNPKTGQSVKPTGL
                     GAAPLELGPDQDPRQSMVDWMAAKDNPFFSRSLVNRYWKHFFGRGLVDPEDDMRVTNP
                     ASNPELLEALAKDFVEKRFDLKQLCRTICTSTTYQLTAEPNEWNQDDKQNFSRYYPKR
                     LNAEVLLDAIDQVTGTQTSFGGIPAGTRAVQLPDNGFNSYFLTVFGRPEAASACECER
                     SSEANLAQSLHLLNSSEIQGKLTAGNGSAARMANDKARPHEVKIRELYLLTFSRVPTP
                     EEMTIALAHINKNEMDPKRAYEDIVWALVNTKEFLFNH"
     gene            3511..6594
                     /locus_tag="VFB80_17805"
     CDS             3511..6594
                     /locus_tag="VFB80_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35692.1"
                     /translation="MKQGSFLGVVTCALLVVAQAVHAQLPTTQLTSIFPPGGKQGTAV
                     EVTVAGNDMDDIDRLAFNHPGLTAAAKMTAATDLEPARPMPGQFTVTIAGDVPPGVYE
                     ARVQGRFGLSNPRPFSVGVQGEIVDNGGNTSPDKAVDVAVGSTVSGRVDANAYDFFRL
                     NLKQGERVVINLVADRIDSRLDGTLVLLSPSGRELTRVKDGVATDPVLDFTAPAEGAY
                     LLRLSDAVYGGGADYFYRLTVSAAPFVDFVFPPAGPAGSNNQYTIYGRNLPGGQPADG
                     LLLGGAPLQKVQVNIAIPGDDAGQSRLPLGELAQPRQAWLDGVEFRLPTPAGPANPVA
                     VYVAKAAVALEQEPNNEAKAATKVTLPCEIAGQFYPQRDLDWFQFDAKKGEIYFVEAI
                     SNQLGLESDPALAIFRVTKNDKGEEQLAEIVQVDDADTRGGGQRRGDGNPGGEFDASH
                     DDPATKFTVPEDGTYRLMLRDQFGDGRQNPAYVYRLAIRPAAPDFRLLAYATVPRNQQ
                     QDNQMPLAAPAIRKGGTLAFTLAVQRRDDFDGEIAVSVEGLPAGVTCPGAVLGGDVSR
                     GALVLVAADSAAAWAGPIKVVGKSKIGDREVVREARYGLIVWGSQNRQQQPPTFRLAG
                     GMQLGVIDKETDPALVQIGEDKVWETSLGGKLEIPVTVTRRGDFKDAIKLTAAGLPQQ
                     IRPKELNVDGNKGDGKFELELNQQNTPLGVYTFYVRGETKWKYPRNPDAIMQAEAEQK
                     RVAEMIKTIEGDVKNATAAKDQATKAAADAAAAAKTGEQKKTEAANNAKAKADAAKQA
                     TDKLAQAKEAAAKDTANAGLAEAAKVAETAANDAAAAQKKADEELAAADKALTDAQAA
                     AKKADEAKVAGEAALKTVTDKQNQANQLKQTADKRVNDTKQQNQPKDVQFALASTPVK
                     LRIHSHPFKVTATPPGGPVKQGEKQELPVKVERLFGFADSVELTLEPPQGVQGLAAEK
                     VTLNKDQGDGKLQVAAAANATPGQHNCTLRVRGRFNNIQVDATTPVTVAVEEVKK"
     gene            6644..10354
                     /locus_tag="VFB80_17810"
     CDS             6644..10354
                     /locus_tag="VFB80_17810"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN35693.1"
                     /translation="MTFSRSVYFTLAALLACLPRVGLAAPIDIAEIKHEGDVDFEKEI
                     LPIFRRNCLACHSATEAQSDLVLESPQTILKGGSEGPAVVAGKSGESLLLKMASHSKE
                     PFMPPPDNDVKAKPLSPMDLGLIKLWIDQGAKGEVKAANAQITWQPLPSGINPIYAVA
                     ITPDGQYAAASRANQIFLYHVPSKRELGRLTDPSLLERGVYKQPGVADLDLVQSLRFS
                     PDGSLLASGGFRTVKIWQKPAGGKKLDLAALEGPARSIAVSSDGKFAAIGEETGKVKV
                     FDLSNGQIVKTLAGHGGPVTGVAFTSDGAKLITGSQDKTFRVFNLADQQQVASIETPA
                     PVNAVAIVAEGKQVATGGADNVIRLWDLPAMQPAEPAKPVKELAGHGGPVTALVALAP
                     NGAQLLSGSKDGTVRQWDVAAGNAVKNVNHGAPVEAIAARADGKRFASVSSANNAKLW
                     NAENSQQVAEMKGDIRASLKVDAANRAVALAKKHIDLAKKDLEEANNRKKAEEENQKK
                     AMEALTKADGEFKPKDEAAKKATEEKTAADKALADATAAKPKADDAKKAADEAFTKAD
                     EAQKKAKTDQDNAAKVATDAAAAKTKADDAKKAADDAFTKADDAQKKAKTDQDNAAKA
                     ATDAAAAKTKADDAKKAADDAFTKADEALKKAKTEQDNAAKAATDAAAAAKAAAEKLA
                     AAKDAASKNPNDQALKDAQAAAEKAATEADAKKKAADDAKVAADKAFTDAEAGRKTAE
                     TNKTAADKAAVDADAAKKTADAAKVAADKVFTDAETARKTAETNKAAADKAVVDADAK
                     KKVADDAKVAADKVFTDADTARKTAETNKGAADKAATDAKTAFDQADQKVKQIAPNHQ
                     KAIDEQTAAQRTLDTAKRGVDRAAESVKKATDSVPGVEAIVKTREEAAQGKEQEQTAA
                     ANAVKESEKPLKGVAFSPDGSLLVTVGDDQLLHSWDAETGAAVEVYGGAAAPLSAIAF
                     TSDGRAITGAANNTGALWDFATEWKLVRTIGTPEAGGPLVDRVTALDFSPDGKVIAVG
                     SGEPSRSGEIKLFTVENGNPVLALKEPHSDTVNSLAFSPDGQQLASCAADRFVKLWNV
                     ADGKFVRSFEGHTHHVLGVSWRADGRMLVSSGADMVLKVWDTRTGDQLRTVQNQFTKE
                     VTSVCFVGDGDLVVASGGDARVRFINAANGNNQRDFGGTADYMYCAAASADGKTVVAG
                     GLDSVLRIWNDQGQELAKFSPPPPPQNQTAAK"
     gene            complement(10356..11372)
                     /locus_tag="VFB80_17815"
     CDS             complement(10356..11372)
                     /locus_tag="VFB80_17815"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN35694.1"
                     /translation="MTWIAGWRWCAAAALLFVAGSARAEQPLVPEHGTAEFVPTTTEG
                     QVGKRFQQAAHSFTWEARRLSAVSENFEVYDVRFPSPVKTPVEANNTVHCEYYRPLRE
                     GPRPGVIVLHILGGDFALSRLFCNSLAQHGVGAIFLKMPYYGPRRDPKSPARMVSADP
                     RATVEGLTQAVLDIRRGTAWLASRPEIDGEQLGLFGISLGGITGGLAAASEPRLKNVC
                     LVLAGGDVARIGWESPELRGVKEKWLAGGGSREQFIELLREVDPVTYAAAARGKHILM
                     LNATNDEVIPKACTEALWRALGQPEIQWYPGGHYSVMRHLFGALTRVSLFFAPPRTPD
                     APQR"
     gene            complement(11369..11704)
                     /gene="cutA"
                     /locus_tag="VFB80_17820"
     CDS             complement(11369..11704)
                     /gene="cutA"
                     /locus_tag="VFB80_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZN35695.1"
                     /translation="MRVEALAVVTTTAEKQDAEKLAQAVLERRLGACVQISGPIESRY
                     WWNSRIETAQEWLLTIKTRRDLYKPLEKLLLDLHPYDQPEIMATAVAEVSAGYLKWLS
                     EQVKEGKSA"
     gene            12299..12478
                     /locus_tag="VFB80_17825"
     CDS             12299..12478
                     /locus_tag="VFB80_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZN35696.1"
                     /translation="MKRYLIEFLRDESGPTATEYAVMLALIVGTCLVSIGFFGTSAGG
                     SWFDTSARIDAAMNN"
     gene            12531..14225
                     /locus_tag="VFB80_17830"
     CDS             12531..14225
                     /locus_tag="VFB80_17830"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZN35697.1"
                     /translation="MTAIVGCEQAGPQPAWPAQPPTPAQQSVGLVRQFVERTGQLYSL
                     PAVAVEVLRLTGEPRVDARSLKDCIERDPALTARILRVVNSSLFGVSRPVTDLSQALA
                     LLGTRPLKMLVLGFSLPKELFSGLEASVLGRYWRRSLVKAVAARDLAEKLWQTSGDEP
                     FIAGLIQDIGSLALIQHLGPSYIQLLDHVQSHGGNLLDRELDTLGFDHVVLSSRLLAH
                     WGLPASLCAAVSAPADEARIAQLEPGQRTLTQVLHLAELLARLIEQPFGPALRDLLNV
                     GARYCGLTYDKLQALVSAVQTKVNELAEVLALELPAGSSYVDLLIAAQQRLSDESAMA
                     AVELAGTGREDQLLSLAGQMRRDLQLAAERGPLHAPPLAPTLTETPTEPFVRSPLRGP
                     PPEAVRRSQPFPVPAAVEDVGLSGLVTAAMGRCRQARCPLSLALFQIDGFGELLLHVG
                     PGVTTELTHWLQSALSEWTAQPAVARHVSDSSLAVALEDCPRSEAVRLARHVLEAVKP
                     WAAARFDSAKLTLSAGLATLEFPPKNFPPHELIDGALRCLSGAELSGGDTVKSIEL"
     gene            14390..15580
                     /locus_tag="VFB80_17835"
     CDS             14390..15580
                     /locus_tag="VFB80_17835"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZN35698.1"
                     /translation="MPSAVTRYITKELLGWFSLWLVLLTLLVIIILVGQEAVRTNLGL
                     GPILRLLPFVLPTSLAYAVPGTILFTVCLVYGRMSADNEVLATKALGISPLALLWPAY
                     ILAFVLSLVGVWLNDLSYSWGQLGVQRVVIQSIEEIVYGMLRTQKSYANQRFSIVVKD
                     VQGRKLIKPFITFQAGRDMPVVTISAEEAELQTNLSRNTMTLILDRFELKVGDTAHGY
                     IPTREAREYPLEFLSARDSRQGNPANLPISQIAGETAAQVRLIEELEQSLAAEAALAL
                     VTGEFGELRDATWKARRSQLTAARGRLYRLQTEPWRRWAAGFSSLAFVLVGAPLAILL
                     RKSDVMTIFGGAFVPILIGYYPLFAGCIDRVKAGALPPCTVWTANLLLAAVGVWLLRR
                     VIRY"
     gene            15692..16537
                     /locus_tag="VFB80_17840"
     CDS             15692..16537
                     /locus_tag="VFB80_17840"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN35699.1"
                     /translation="MPAPKRQAPIEVTLPAYGVYLLESHHAPSFRMGEQSHSFLELFF
                     VHSGSGAFRIAGRDHPCQRNDLVVVPPRMTHSIADNPAHPLTLYAVCVAAAVVRHEPA
                     IFERLPVGRVRIGQALADETRATFRRLLYEQTQNQPFGPTLIVGLTLELLASLARATA
                     VETSPPASDAPTAERRAAVERYVAALSHRFFERMSLDEAAAELGMSRRRFTTLFSEVT
                     GRTWADYLAELRVEYAQTLLRDTPRSIVSIAFECGYEELSSFYRAFKRATGNSPGDWR
                     AEQRA"
     gene            16682..>17013
                     /locus_tag="VFB80_17845"
     CDS             16682..>17013
                     /locus_tag="VFB80_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35700.1"
                     /translation="MALSRHAVRPRAGFTLVELLVVIAIIGVLVALLLPAVQAAREAA
                     RRSSCQNNLKQIGLATHNFHDVYNQLPPLRVNNNRATWFVLIMPYMEQENIERLWRYD
                     QDYSNAANV"
BASE COUNT         3121 a         5766 c         5588 g         2538 t
ORIGIN      
        1 cggcacgatg atctccgccg tgtcacgagt cgaagccgat ggctcgatcg tcagcgactt
       61 gaagtttgag cggagcctgg tcacgccgcg gcggcaaccc gaagggggcg acccggcggc
      121 catcacgccg caaggcaccg caagcgtcaa cgtgcggacc acggtccggg ccaggcccgg
      181 tgagccggtg ctcgtcggcg gccggcaaca cggcagcggc aaagacgcag cccaaacctg
      241 gctcgtgctc acggccagcg tcgcgccccg cgctgcgggc gagaaaccga aggcggcagc
      301 ggcggcggga cccgaggaag agctgaaaat cgtccagctc aagaacgcct cggcaccggc
      361 tctcgccact gtcctggcca gcgtgtttca gcgcgagttt cggattgcgg tcgatgaacg
      421 gacgaactcg ctcctcttgc gcgggtcgcc cgaacgcctg gttattgccg tggcgctgat
      481 tgcccggctg gacgagtcgt agtcctggcg ttctacttca ctgctgtatt ctgaaggccg
      541 cggcgggttg ctgcggcctc gccgttttgc tccccggagt aacggcgcgg gtaaactggt
      601 catgtagtcg ttaagggcgt tgctgccggg atttatgacc ggccacgacc catcaacaaa
      661 ttcgaatatt tgcttgcaaa ggcagccgcc aggcgcgata atcgtgcgca agttcgtgcc
      721 tggcctgata atggagcacg cctgacctga tggaacaact cgcgaaggaa ccttccatgc
      781 gatcccgcct gaccgcggcc ccccgcttgg ccgcactgtt cgcagccgcc ttcctcctga
      841 cccacgccaa cgccgccgac accccaaata agcccaaggc ccccggtctg ggcgacccgg
      901 gcaagttggc ggccatccag atcgaaaccg gccgcctcaa ggacggcctg gtgacggtgt
      961 cgggccgcga tgcggggcag cagatcgtcg tcaccggcca gtacgacagc ggacagaccc
     1021 gcgacttcag ccgcaagtgc acctatgaag tgtcgccggc gggcattgtg aacgtcgatt
     1081 cgaccggcct cgtcacgccc gtggcggaag gcgaagcgac ggtccacgtc gtcgccgagc
     1141 cgggcatcga cggcaacctg aaggtcaagg tgacgaacct cgtcaccgac ttgccgatca
     1201 acttccccaa ccagattacg cccgtcttca ccaaatacag ctgcaacggc ggcggctgcc
     1261 acggcaaatc cggcgggcaa aacggcttcc gcctctcgct cttgggcttc gagcccaagg
     1321 aagactacga atacctgtgg aaggaaggcc gcggccggcg gctctttccc gccgcgcccg
     1381 accagagcct gctgctcctg aaggccacgg cccgcctgcc gcacggcggc ggccagcgga
     1441 tcgagttcga ttcgccagcc taccgcctgc tgaagcggtg gatcgagcag ggcatgccct
     1501 acggcaagga caccgatccc aaggtgacgc acatcgaagt gctgcccgcc gagcggctga
     1561 tggagcgcga ggggacgcag caaatcgtcg tcgtcgccca ctacagcgac ggcagcagcg
     1621 aagacgtcac ccgcacgacg cagttcgaca gcaacgacac ggaaatggcc gaggtcagcg
     1681 tctcgggcct ggtcaccacg agccagctca ccggcagcgt ggccgtgatg gcccgctatc
     1741 aggggcacgt cggcgtattc cgggcgacga ttccgctggg tgtgaagacc gacaatctgc
     1801 cgccgggcaa gaacttcgtc gatgagttcg ttcaaaagaa gctgaagtcg ctgggcttgc
     1861 cgtcgtcggc gatttgcgac gacgccacgt tcctgcggcg ggtcagcgtc gatctggccg
     1921 gccggctgcc gaccttggag gagaccgagc aattcctggc cgacgccgcg cccaacaagc
     1981 gcgagaacct ggtcaaccgc ctgctcgaca gcaccgacta cgccgattac ttcgccaaca
     2041 agtggtcggc cattttgcgg aacaagcggc ggcaggatac cgagaagctc tcgacgtttt
     2101 cgttccacga gtggattcgc cagagcctcc atgagaacaa gccctacgac caattcgccc
     2161 gcgaaatcct gacggcgacg ggcacggcca gcgtcaatcc gcccgccggc tggtatcgcg
     2221 aggtgaagga cgcctcggcg cagctggaag acaccgcaca gctcttcctg ggcctgcgga
     2281 ttcagtgcgc caagtgccac caccatccgt tcgagaagtg gagccagcag gactactacg
     2341 gtttctcggc gttcttttcg caggtcggcc gcaagcgggg tgacacgcag gactccgaga
     2401 agatctttca ccgccgcggc gtggcgcagg cccagaatcc caagaccggc cagagcgtca
     2461 agccgacggg cctgggcgct gctccgctgg agcttggccc cgatcaggac ccgcggcagt
     2521 cgatggtcga ttggatggcg gcgaaggaca acccgttttt cagccgctcg ctggtgaacc
     2581 gctactggaa gcacttcttc ggccgcggcc tggtcgatcc ggaagacgac atgcgagtga
     2641 ccaatccggc cagcaacccg gaattgctgg aggcactggc caaggacttt gtcgagaaga
     2701 gattcgacct gaagcagctg tgccggacaa tctgcacctc cacgacgtac cagctcaccg
     2761 ccgagcccaa cgagtggaat caagacgaca agcagaactt ctcgcggtac tatcccaagc
     2821 ggctcaacgc cgaggtgctg ctggacgcga tcgaccaggt gaccggcacc caaacgagct
     2881 tcggcggcat ccccgccggc acgcgggccg tgcagttacc ggacaacggg ttcaactcgt
     2941 acttcctcac cgtcttcggc cggcccgagg cggccagcgc ctgtgagtgc gagcgctcca
     3001 gcgaggcgaa cctcgcccag agcctgcacc tgctgaactc cagcgagatc cagggcaagt
     3061 tgacggccgg caatggcagc gcggcccgca tggccaacga caaggcccga ccgcacgagg
     3121 tgaagatccg cgagctgtac ctgctcacgt tctcgcgcgt gccgacgccg gaggaaatga
     3181 ccattgccct ggcccacatc aacaagaacg agatggaccc caagcgggcc tatgaagaca
     3241 tcgtgtgggc gctcgtgaac acgaaggagt tcctgtttaa ccactagaga actcggtccc
     3301 aagcaccgct tgggaccgca cggccggaag ctccgcttcc cgggcacgaa gcagagcttc
     3361 gaagacttgt gttcccaagc ggagcttggg aacaagtagc gcgtccgccc gtttgcacaa
     3421 atctcgccat tcaatcacac tgaatgacca acgagcccgc ctgaccgact cccgcctgac
     3481 cgaacatacc ctcccctgga gcggaccacc atgaaacagg gcagtttcct cggcgtcgtc
     3541 acctgcgctt tgctcgtggt agcccaggca gtccacgctc agcttcccac gacgcagctc
     3601 accagcatct tcccgccggg cggcaaacag gggacggccg tcgaggtcac cgtcgccgga
     3661 aacgacatgg acgacatcga ccggctggcg ttcaaccacc cgggactcac cgcggcggcc
     3721 aagatgacgg cggccacgga cctcgagccg gcgcggccga tgcccggcca gttcaccgtg
     3781 acgatcgccg gcgacgtgcc cccgggagtc tacgaggccc gcgtgcaggg gcggttcggc
     3841 ctgtcgaacc caaggccctt cagcgtcggc gtgcagggag agattgtcga taacggcggc
     3901 aacacgtcgc ccgacaaggc cgtggacgtg gcggtgggct ccaccgtcag cggccgcgtg
     3961 gatgcgaacg cgtacgactt tttccggctg aacctgaagc agggagagcg ggtggtgatc
     4021 aacctggtcg ccgaccgcat cgattcgcgg ctggatggca cgctggtcct gcttagcccc
     4081 agcggccgcg agctgacccg cgtgaaggac ggcgtcgcca ccgacccggt gctcgatttc
     4141 accgcgccgg ccgagggcgc gtatctgctg cggctgagcg acgcggttta tggcggcgga
     4201 gccgactatt tctatcgcct cacggtcagt gccgcgccgt tcgtcgattt cgtctttcct
     4261 ccggccggac cagcgggctc gaacaaccag tacacgatct acggccgcaa tctgcccggc
     4321 ggacagccgg cggatgggct gctcttgggg ggcgcgccgc tccagaaggt gcaggtcaat
     4381 atcgcgatcc ccggcgacga cgcgggccag tcgcggctgc cgctgggcga attggcccag
     4441 ccgcggcaag cctggctcga cggcgttgag ttccgcctgc cgacgcccgc gggtcctgcg
     4501 aacccggtcg cggtgtacgt cgccaaggcg gcggtcgcgc tggagcagga gccgaacaac
     4561 gaggccaagg ccgcgacgaa ggtgacgctg ccctgcgaga tcgccggcca gttctatccg
     4621 cagcgcgacc tggactggtt ccagttcgac gccaagaagg gggaaatcta tttcgtcgag
     4681 gcgatctcga accagctcgg cctggaaagc gatccggcgc tggcgatctt ccgcgtcacc
     4741 aagaacgaca agggggagga acagctcgca gagatcgttc aggtggacga cgccgacacg
     4801 cgcggcggcg ggcagcgccg cggcgacggc aatcccggcg gcgaattcga cgcctcgcac
     4861 gacgacccgg ccacgaagtt caccgtgccc gaggacggca cctaccgctt gatgctccgc
     4921 gaccagttcg gcgacggccg ccagaacccg gcctatgtct atcgcctggc gatccggccg
     4981 gccgcgcccg acttccgcct gctggcctat gccaccgtgc cccgcaatca gcagcaggac
     5041 aaccagatgc cgctggccgc gcccgcgatc cgcaaaggcg gcacgctggc cttcacgctc
     5101 gcggtgcagc gccgcgacga tttcgacggc gagatcgcgg tcagcgtgga agggctcccc
     5161 gctggcgtga cctgtccggg cgcggtgctc ggcggcgacg tcagccgcgg ggccctggtg
     5221 ctcgtcgcgg ccgactcggc ggcggcgtgg gcggggccga tcaaggtcgt cggcaagtcc
     5281 aagatcggcg accgcgaggt cgtccgcgaa gcccgctatg gcctgatcgt gtggggctcg
     5341 cagaaccgcc agcagcagcc gccgaccttc cgcctggccg gcgggatgca gctcggcgta
     5401 atcgacaagg agaccgatcc ggccctggtg cagatcggcg aagacaaggt ttgggagact
     5461 tcgctgggcg gcaagctgga gattccggtc accgtcaccc gccgtggcga cttcaaggac
     5521 gcgatcaagc tgacggccgc cggcctgccg cagcagattc gccccaagga gctgaacgtt
     5581 gacggcaaca agggggacgg caagttcgag ctggaattga atcagcagaa cacgccgctg
     5641 ggcgtttaca cgttctacgt ccgcggcgag acgaagtgga agtatccccg caatccggac
     5701 gcgatcatgc aggccgaggc cgagcagaaa cgcgtcgcgg agatgatcaa gacgatcgaa
     5761 ggggacgtga agaacgcgac cgcggccaag gaccaggcga ccaaagccgc cgccgatgcg
     5821 gccgccgccg ccaagaccgg tgagcagaag aagaccgagg ccgccaacaa cgccaaggcc
     5881 aaggccgacg ccgccaagca ggcgaccgac aagctcgcgc aggccaagga ggcggccgcc
     5941 aaggacacgg cgaacgcggg cctggccgag gcggccaagg tggcggaaac ggctgccaat
     6001 gacgccgccg cggcgcagaa aaaggcggac gaagagctgg ccgccgccga caaggccctg
     6061 accgacgcac aggccgccgc caagaaggcg gacgaggcca aggtcgccgg cgaagcggcg
     6121 ctgaagaccg tcaccgacaa gcagaaccag gccaaccagc tcaagcagac agccgacaag
     6181 cgggtgaacg acaccaagca gcagaaccag cccaaggacg tgcagttcgc gctggccagc
     6241 acgccggtga agctgcggat tcactcgcac cccttcaagg tgacggctac gccgccgggc
     6301 ggccccgtca agcagggcga gaagcaggaa ctgccggtga aggtcgagcg gctgttcggc
     6361 tttgcggact ctgtggagct gactctcgag ccgccgcaag gcgtgcaggg gctggcggcc
     6421 gagaaagtga cgctgaacaa ggaccagggg gacggcaagc tgcaagtggc cgccgcggcg
     6481 aatgccacgc cgggccagca caactgcacc ctccgcgtcc gcggccggtt caacaatatc
     6541 caggtcgatg ccacgactcc cgtgacggtc gcggtcgagg aagtaaagaa gtaattcgtt
     6601 tcagacagcc atacacgaaa tcctcgcgga caggattctc ccgatgacgt tttcccgctc
     6661 tgtgtatttc acgctcgccg cactgctcgc ctgtttgccg cgcgttgggc tggcggcgcc
     6721 tatcgatatc gccgagatca agcacgaggg cgacgtcgat ttcgagaagg aaatcctgcc
     6781 gatcttccgc cgcaactgcc tggcctgcca cagcgccacg gaggcccaga gcgacctcgt
     6841 gctggaatcg ccacagacga tcctgaaagg cggcagcgaa ggtccggcgg tcgtcgccgg
     6901 caagagcggc gagagcctgc tgctcaagat ggccagccac agcaaagagc ccttcatgcc
     6961 gccgccggac aacgacgtga aggccaagcc gctctcgccg atggatttgg gcctgatcaa
     7021 gctctggatc gaccagggcg ccaaaggcga ggtcaaagcc gccaatgccc agatcacctg
     7081 gcagccgctg ccgagcggca tcaatccgat ctacgctgtc gccattacgc ccgacgggca
     7141 atacgcggct gcgtcgcggg ccaaccagat tttcctctat cacgtgccga gcaagcgcga
     7201 gctgggccgc ctgaccgatc cgtcgctgct ggagcgcggc gtctataagc agccgggcgt
     7261 cgcggatctg gacctggtgc agtcgctccg gttcagcccc gatggcagct tgctggccag
     7321 cggcggcttc cgcacggtga agatctggca gaagccggct ggcggcaaaa agctggacct
     7381 ggcggccctg gagggcccgg cccgttcgat cgcagtcagc agtgacggca aattcgcggc
     7441 gatcggcgag gagaccggca aggtcaaggt gttcgacctg agcaacggtc aaatcgtgaa
     7501 gacgctcgcc ggccacggcg gtccggtgac cggtgtcgcc ttcacgtccg acggcgcgaa
     7561 gctcatcacc ggctcgcagg acaaaacctt tcgcgtgttc aacctggccg atcaacagca
     7621 agtggcctcg atcgagacgc ccgcgccggt caatgcggtg gcgatcgtcg ccgaaggcaa
     7681 gcaggtcgct accggcggcg cggacaatgt gattcgcctg tgggacttgc cggccatgca
     7741 gccggccgag ccggccaagc cggtgaagga gctcgctggt cacggcgggc cggtgacggc
     7801 gctggtcgcg ctggccccca atggcgcgca gctgctctcg ggcagcaagg acggcaccgt
     7861 gcgtcaatgg gacgtcgccg cgggcaatgc cgtgaagaac gtgaaccacg gcgctccggt
     7921 cgaggccatc gcggcgcggg ccgacggcaa gcggttcgct tcggtcagct ctgcgaacaa
     7981 tgccaagctg tggaacgccg agaacagcca gcaagtcgcc gagatgaagg gcgacattcg
     8041 tgccagcctg aaggtcgatg ccgccaaccg cgccgtcgcc ctggccaaga agcacatcga
     8101 tctggccaag aaggatctgg aggaggccaa caaccgcaag aaggccgaag aagagaacca
     8161 gaagaaggcc atggaggccc tgaccaaggc cgacggcgag ttcaagccga aggacgaagc
     8221 cgccaaaaag gcgacggagg aaaagacagc cgccgacaag gccctggccg atgccaccgc
     8281 cgccaagccc aaggccgacg acgccaagaa ggcggcggat gaggccttca cgaaggccga
     8341 cgaggcccag aaaaaagcca agaccgatca ggacaacgcc gccaaggtcg cgactgatgc
     8401 cgccgcggcc aagaccaagg ccgacgacgc caagaaggcc gcggacgacg ctttcaccaa
     8461 ggccgacgac gcccagaaga aggccaagac cgatcaggat aacgccgcca aggccgccac
     8521 cgatgccgcg gcggcgaaga ccaaggccga cgacgccaag aaagcagcgg acgacgcctt
     8581 cacgaaggcc gacgaagcgc tgaagaaagc caagaccgaa caggacaacg ccgccaaagc
     8641 cgcgaccgac gcggctgccg cggccaaggc tgccgccgag aaactggccg ccgccaagga
     8701 cgcagcttcc aagaatccca acgatcaggc gctgaaagac gcccaggccg cggccgagaa
     8761 agctgcgacc gaggccgacg ccaagaagaa ggctgcggac gacgccaagg ttgccgccga
     8821 caaggccttc accgacgccg aggccggccg caagaccgcc gagaccaaca agaccgccgc
     8881 cgacaaggcc gcggtggatg ccgacgccgc gaagaagacg gccgacgccg ccaaagtggc
     8941 cgcggataaa gtcttcaccg acgccgagac cgcccgcaag accgccgaga cgaacaaggc
     9001 cgccgccgac aaggccgtcg tcgatgcgga cgccaagaag aaggtcgccg acgatgccaa
     9061 ggtggccgcg gacaaggtct ttaccgacgc cgacacggcc cgaaagacgg ccgaaacgaa
     9121 caaaggggct gccgacaaag cggccacgga tgccaagacg gccttcgacc aggcggacca
     9181 gaaagtgaag caaattgcgc cgaaccatca gaaggcgatc gacgagcaga ccgccgccca
     9241 gcggacgctc gacacggcca agcgcggcgt cgatcgcgcg gccgagtcgg tcaagaaggc
     9301 aaccgactcg gttcccggcg tggaagcgat cgtgaaaacc cgcgaggaag ccgcccaggg
     9361 caaggagcag gagcagaccg ccgccgccaa tgccgtcaag gagagcgaga agccgctgaa
     9421 gggcgtcgcg ttctcgcccg acggctccct gctagtcacc gtcggcgacg accagctgct
     9481 gcacagctgg gacgccgaaa ccggcgcggc cgtcgaggtc tacggcggcg cggccgctcc
     9541 gctgagcgcc atcgcgttca cgtcggacgg ccgggcgatc accggcgctg ccaataacac
     9601 cggcgccctg tgggacttcg ccaccgagtg gaagctcgtc cgcacgatcg gcacgcccga
     9661 ggccggcggc ccgctggtcg atcgtgtcac cgccctcgac ttctcgcccg acggcaaggt
     9721 catcgccgtc ggcagcggcg agccctcgcg gagcggcgag atcaagctgt tcaccgtcga
     9781 gaacggcaat cccgtgctgg ccctcaagga gccgcacagc gataccgtca acagcctggc
     9841 gttttcgccc gacggccagc agctcgccag ctgcgccgcc gaccgcttcg tgaagctgtg
     9901 gaacgtggcc gacggcaagt tcgttcgctc gttcgagggc cacacgcatc acgtgctggg
     9961 agtctcgtgg cgggccgacg ggcggatgct cgtctccagc ggggccgaca tggtgctcaa
    10021 ggtctgggac acgcggaccg gcgaccagct ccgcacggtg cagaaccagt tcaccaagga
    10081 agtcacgagc gtctgcttcg ttggcgacgg cgacctggtg gtggccagcg gcggcgacgc
    10141 gcgggtgcgg ttcatcaacg ccgccaacgg caacaaccag cgcgacttcg gcggcacggc
    10201 cgactatatg tactgcgccg cggccagcgc cgacggcaag acggtcgtcg ccggcggcct
    10261 cgacagcgtg ctccgcatct ggaacgacca ggggcaggaa ctggccaagt tctctccgcc
    10321 gcccccgccg cagaaccaga ccgcagcgaa gtagcctatc gctgcggcgc gtcgggcgtc
    10381 cgcggcgggg cgaagaagag agagacgcgg gtcaaggcgc caaacaagtg ccgcatgacg
    10441 ctgtagtggc cgcccgggta ccactggatc tcgggctggc ccagggccct ccacagcgcc
    10501 tccgtgcaag ccttgggaat cacctcgtcg ttggtggcgt tgagcatcag gatgtgcttg
    10561 ccgcgcgccg ccgcggcgta ggtcaccgga tcgacctcgc gcagcagctc gatgaactgc
    10621 tcgcgggagc cgccgccggc gagccatttc tccttgacgc cgcgcagctc cggcgactcc
    10681 cagccgatgc gggccacgtc gccgccggcc agaaccaggc agacgttctt cagccggggc
    10741 tcggacgccg cggccaggcc gccggtgatg ccgcccaggc tgatgccgaa caggcccagc
    10801 tgctcgccgt cgatctccgg ccgtgaggcc agccaggcgg tcccgcggcg gatgtcgagc
    10861 acggcctgcg tcaggccttc gaccgtcgcc cgcgggtcgg cgctgaccat gcgcgccggc
    10921 gacttcgggt cgcgacgcgg gccgtagtag ggcatcttca ggaatatcgc ccctacgccg
    10981 tgctgggcca gcgagttgca gaacagccgc gacagcgcaa agtcgccgcc caggatgtgc
    11041 aggacgatga cgcccggccg cgggccctcc cgcagcgggc gatagtattc gcagtggacc
    11101 gtgttgttgg cctcgaccgg cgtcttgacc ggcgagggaa accgcacgtc gtagacctcg
    11161 aagttctcgg acaccgccga gaggcggcgg gcctcccagg tgaagctgtg ggccgcctgc
    11221 tgaaagcgct tgccgacctg gccctcggtc gtggtcggca caaactccgc cgtgccgtgc
    11281 tcgggcacga gcggctgttc cgcacgagcc gatcccgcga cgaacagcag cgccgccgca
    11341 gcgcaccacc gccagcctgc gatccaggtc atgccgattt tccctctttg acctgctcgc
    11401 tcagccattt gagatagccc gcactgactt cagccaccgc cgtggccatg atctccggtt
    11461 gatcgtaggg atgcaaatcc aaaagcagct tttccagcgg cttgtacagg tcgcggcggg
    11521 tcttgatcgt cagcagccac tcctgcgccg tttcgatgcg gctgttccac cagtagcggc
    11581 tctcgatcgg tccgctgatc tgcacgcatg cgccgagccg ccgttcgagc accgcctggg
    11641 ccagcttctc ggcatcctgc ttttcggccg tcgtcgtaac cacggccaga gcttcgactc
    11701 gcataccaac atactccacc tacggatgac cgaatcctag cagcgtcctc atatccggca
    11761 attccactct gccgatcgca tcccggcgcc gtccgtctcg ataaacttcc aggaagcttt
    11821 ccgccgggaa acacgccatg ccacgcttgc tgctagcgct attcgccagt ctgacgctcg
    11881 tgacgattgt atcgcccgcc cgcgcccatt ggggcagctg cgggcggcac ctgggctgcg
    11941 ggtggagcga cggctatcac gccgccaacc tttgtgccgc aaagtgtccc agctgcactt
    12001 ccgcgccctg gtggatgtcg ccgctacaag ccgaaccgct agccagtcca ggctctccag
    12061 cacaattccg cggaccgcta ggaccctcac tcttccgtca gccgggcgcg gggtcgtccg
    12121 tcatcgttac ggacggacct ctggtgagcc cctgagccga attcgcggaa atctgcaccg
    12181 caaaaattgc ttttgccgcg ctgcatcgta gggtttggtt gcaccaccat agtcgcggcc
    12241 aatagtccgg cgacagcgac ttggcggcgc cgcgggtgta ttggcgagga ggatgttcat
    12301 gaagaggtac ttaatcgagt ttctgcgaga cgaaagcggc cccaccgcca ccgaatatgc
    12361 ggtcatgctg gcgctgattg tcggcacctg cctggtcagc atcggcttct tcggcacgtc
    12421 ggcgggcggc agctggttcg atacgtccgc gcggatcgac gccgccatga acaactagcc
    12481 gcgagcggcc gggcggcttc tctgtgtgct acggttcacg ggaggaccgc atgaccgcca
    12541 tcgtcggctg cgaacaggcc gggccgcagc ccgcctggcc ggcccagcca cccaccccgg
    12601 cgcagcagtc cgtcggactc gtgcgccagt ttgtggaacg caccgggcag ctctattcgc
    12661 tgcccgcggt tgccgtggag gtcttgcggc tcaccggcga gccgcgcgtc gatgcccgct
    12721 cgctcaaaga ttgcatcgaa cgcgatccgg ccctgacggc ccgcattctc cgcgtcgtca
    12781 acagctcgct gttcggcgtc agccgtccgg tgaccgacct gagccaggcg ctggcactct
    12841 tgggcacgcg gccgctgaag atgctggtcc tgggcttcag ccttcccaag gagctgttct
    12901 ccgggctgga agcgagcgtg ctgggccgct attggcggcg gtcgctggtc aaggccgtcg
    12961 cggcccgcga cctggccgaa aaactctggc agaccagcgg cgacgagccg ttcatcgccg
    13021 ggctgatcca ggacatcggc tcgctggcct tgattcagca tctcggcccg tcgtacatcc
    13081 agctgctcga ccacgttcag tcccacggcg gcaacctgct cgatcgcgag ctggatacgc
    13141 tcggcttcga tcacgtcgtg ctctcgtcgc ggctgctggc ccactggggc ctgccggcga
    13201 gcttgtgcgc ggcagtgtcc gccccggcgg acgaggcgcg catcgcgcag ctcgagccgg
    13261 ggcagcgcac gctgacccag gtcctgcacc tggccgaatt gctggcccgg ctgatcgagc
    13321 agccgttcgg accggcgctg cgcgacctcc tgaacgtcgg ggcgcggtat tgcggcctga
    13381 cctacgacaa gctgcaagcc ttggtctctg cggtgcaaac caaggtgaat gagctggccg
    13441 aagtgctggc gctggaattg cccgccggca gcagctacgt cgatctgctg atcgcggcgc
    13501 agcagcgcct gtcggacgaa agcgcgatgg cggcggtcga gctggccgga accggtcgcg
    13561 aggaccagct gctgtcgctg gccggccaga tgcgccgcga cctgcagctg gccgccgagc
    13621 gcggccctct gcacgcgccg ccccttgcgc caaccctgac cgaaactccg accgagccct
    13681 tcgtccgctc tccgctacgc gggccgcctc ccgaagcggt ccgccgcagc cagccgtttc
    13741 ccgtgcctgc cgccgtcgag gacgtagggc tgtctggcct cgtgaccgcg gcgatgggcc
    13801 gctgccggca agcccgctgc ccgctgagcc tggccctgtt tcagattgac ggtttcggcg
    13861 aattgctgct gcacgtcggc cccggcgtga cgacggagct gacccactgg ctgcaatcgg
    13921 cgctctccga gtggaccgca cagcccgccg tggcccggca tgtcagcgac agttcgctgg
    13981 ccgtggccct ggaggattgc ccgcggagcg aagccgtccg cctggcccgg cacgtgctgg
    14041 aagccgtcaa gccctgggcc gccgcgcgtt ttgattcggc caagctgacg ctcagcgcgg
    14101 gcctggcgac gctcgaattc cctcccaaga acttcccgcc gcacgagctc atcgacggcg
    14161 ctctgcggtg cctgagcggg gcggaattgt ccggcggcga taccgtgaag agtatcgagc
    14221 tgtaggtcgg cacgatcctt catcgctccg ttcctctgtc tccctccctc agtcttcccc
    14281 tcccgcccaa ctgaagttgg gccaaatggg ggcttctgct agactccggc tcaatcccgc
    14341 cccctgctgc gccgatgctg accgatgcgc cgcagcctcc ggctccccca tgcccagcgc
    14401 cgttacccgc tacatcacga aagagctgct gggctggttc agcctgtggt tggtgctgct
    14461 cacgctgctc gtcatcatca ttctcgtcgg ccaggaggcg gtgcggacga acctgggcct
    14521 ggggccgatc ctgcggctgc tgccgttcgt gctgccgacg tcgctggcct acgcggtgcc
    14581 ggggacgatc ctgttcacgg tctgcctggt ctatgggcgg atgagcgccg acaacgaagt
    14641 tttggcgacg aaggccctgg gcatctcgcc gctggcgctg ctctggccgg cgtacatcct
    14701 ggcgttcgtg ctcagcctgg tgggcgtgtg gctgaacgat ttgtcgtatt cctggggcca
    14761 gctgggcgtg cagcgggtcg tgatccagtc gatcgaggag atcgtgtacg gcatgctccg
    14821 cacgcaaaag tcgtacgcca atcagcggtt ctcgatcgtc gtcaaggacg tgcaggggcg
    14881 gaagctgatc aagccgttca tcacgttcca ggccggccgc gacatgcccg tcgtcacgat
    14941 ctcggccgaa gaggcggagc tgcaaacgaa cctgtcgcgg aacacgatga cgctgatcct
    15001 cgaccgtttc gagttgaagg tcggcgacac ggcgcacggc tacattccca cccgcgaggc
    15061 ccgcgagtat ccgctggagt tcctctcggc ccgcgacagc cggcagggca acccggcgaa
    15121 cctgccgatc tcgcagatcg ccggcgagac cgccgcgcag gtgcgtctga tcgaagagct
    15181 ggagcaatcg ctggcggccg aagcggcgct ggcgctggtc accggggaat ttggcgagct
    15241 ccgcgacgcc acgtggaagg cccgccgcag ccagctgacc gcggcccgcg gccggctgta
    15301 ccgcctgcag accgagccct ggcggcgatg ggccgccggc ttcagcagcc tggcgttcgt
    15361 gctggtcggc gcgccgctgg cgatcctgct ccgcaagtcg gacgtgatga cgatcttcgg
    15421 cggcgctttc gtgccgatcc tgatcggcta ttatccgctc ttcgccgggt gcatcgaccg
    15481 ggtgaaggcg ggcgcgctgc ccccctgtac tgtctggacg gccaatctgc tcctggctgc
    15541 cgtcggcgtg tggctgctgc ggagggtgat tcgctattga tgtccgccgc aggaggaaaa
    15601 ggaagattag gattaggatt aggattaaga ttaggacggc gattgggagt cgatttcgta
    15661 atcttaatcg taatcttaat ccttctcctg catgcccgct cccaaacgcc aggcgccgat
    15721 cgaagtgacg ctgccggcgt atggcgtgta tctgctcgag agccatcacg cgccgagctt
    15781 ccgcatgggg gagcaatcgc actcgttcct ggagctgttc ttcgtgcaca gcggcagcgg
    15841 cgcgttccgc atcgccggtc gcgatcatcc ctgccagcgg aacgacctgg ttgtcgtgcc
    15901 gccgcggatg acgcactcga tcgccgacaa ccccgctcat cctctgacgc tgtatgccgt
    15961 ctgcgtcgcc gcggccgtcg tccgccacga gccggcgatc ttcgagcgcc tgcccgtcgg
    16021 acgcgtgcgg atcggccagg ccctggccga cgaaacgcgg gccacgttcc gccggctgct
    16081 ctacgagcaa acgcagaatc agccgttcgg cccgacgcta atcgtgggac tgacgctgga
    16141 gctgctggcc tcgctggccc gggccacggc ggtcgagacc tcgccgccgg ccagcgatgc
    16201 gccgacggcc gagcggcggg cggccgtcga gcgctacgtg gcggcgctgt cgcaccggtt
    16261 cttcgagcgg atgagcctcg acgaggcggc cgccgaattg gggatgagcc gccgccggtt
    16321 cacgacgctg ttctcggagg tcaccggccg cacctgggcc gactacctgg cggagctgcg
    16381 ggtcgaatac gcccagacgc tgctgcgcga tacgccgcgg agcatcgtgt cgatcgcctt
    16441 cgagtgcggc tacgaagaac tgtcgagctt ctaccgggcc ttcaagcggg ccaccggcaa
    16501 ttcgccgggc gattggcggg ccgaacagcg ggcctgattg cccgatttgc gaatccggat
    16561 tgcccgccct gccaagaccc aaagcgggcc gccgagcata atcgttggct gtaatggggt
    16621 tcgcggggcg cctggtccga atcgggaatt ctcccgaaga acacttgcaa ggagttcgtg
    16681 aatggccctc tcccgtcatg cggtgcggcc gcgcgccggc tttacgctgg tcgaattgct
    16741 ggtcgttatt gcgatcatcg gcgtcctcgt ggcactgctt ttgcccgcgg tgcaagccgc
    16801 ccgcgaagcg gcccggcgat cgagctgcca gaacaacctg aagcagatcg gcctcgccac
    16861 gcacaatttc cacgacgtct acaatcagct gcccccgctc cgcgtgaaca acaaccgcgc
    16921 cacctggttc gtgctgatca tgccctacat ggagcaggag aatatcgagc gcctgtggcg
    16981 ctacgaccag gactattcca acgcggcgaa cgt
//
LOCUS       DASRJB010000672         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2163449, whole genome shotgun sequence.
ACCESSION   DASRJB010000672 DASRJB010000000
VERSION     DASRJB010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163449"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1673
                     /locus_tag="VFB80_17850"
     CDS             105..1673
                     /locus_tag="VFB80_17850"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN35701.1"
                     /translation="MIRLLAAAIVPLLLVAGASAADRPNVLILLADDMGYSDAGCYGG
                     EVQTPNLDVLATGGLRFTQFYNTARCWPTRGALLTGYYAQQIRRDTVPGIASGNAGTR
                     PKWAVLLPQLLKPLGYRSYHSGKWHVDGQPLAGGFDRSYLVQDQDRFFSPRNHAEDGV
                     ALPQPAADSGYYATRAIADHALKCLRDHAEKHADQPFFHYLCFTAPHFPLHALPEDIA
                     KYRGRFAAGWDAMRVERLARMKKLGIVSCELSPRTAGVPAWDSLSAAEREAWQRHMEI
                     HAAMIDRVDQEIGRVLAQLREMKVLDNTVIFFLSDNGASAEKLVRGDGHDPAAPPGSA
                     KTFMCLEPGWANLANAPLTRSKIFVHEGGISTSLIVHWPAGVAARGELRTNPGHVIDL
                     VPTILELTGGKRPETIEGEPVPPSPGKSLVPVFAKDGSVSRDYLWWYHSGNRAIRVGD
                     WKLVSTGEEGPWELYDLRTDRSESHDLAASQPEKARELAAAWTARLEEFRVLAAKDAP
                     PAPAKKKGAKAKKM"
     gene            complement(1681..2412)
                     /locus_tag="VFB80_17855"
     CDS             complement(1681..2412)
                     /locus_tag="VFB80_17855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN35702.1"
                     /translation="MSFASDLKILYHLALKPVRGKDHAARMENFYSGQAEAYDDFRKR
                     LLKGRQELWNLIPPPAGGTWIDMGGGTGGNLDYFGDSIHNLGKVYVLDLSHSLLEVAK
                     KRIAAKGWTNVETVEADATTYQPPSGPVDVVTFSYSLTMIPDWFAALENALAMLKPGG
                     TIGVVDFYVSRKFASDGLARHGWMTRTFWPAWFAMDNVFPSPDHVPFLQRHFDVVHFQ
                     EHRAKVPYIPLSRVPYYLFVGRKRA"
     gene            complement(2471..3667)
                     /locus_tag="VFB80_17860"
     CDS             complement(2471..3667)
                     /locus_tag="VFB80_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HZN35703.1"
                     /translation="MSVFDWVSGRVFKFVHGNNLVYNTCWEDPRLDRVALAIQPRDSV
                     LVITSAGCNALDYALCGPEHVFAVDMNPRQNALLDLKLAGIKSLQFEDFFRLFGYGYH
                     PDARRLYQRQMRPALPAWSQAWWDRWIKFFDNKARPFYFRGTSGAFARLIKIYTDRLI
                     RVRPHIDAALDAKSLAEQREIYEKHLKQKFWSRPMRFAMNRDTTLSMVGVPKAQRKQV
                     ESQYHGGIVKFVQDAVEAVFCKLPLADNYFWRVYMKGEYSRSCCPEYLKEDNFHKLKG
                     GLADRVSTHTDSVQGFLDKHNGQISKYVLLDHMDWLSDKFFPLLELEWQAILKRAATS
                     SRIIWRSGGLKTDFIDRVNVTTHGQPRMLPELLKFHPQLAAELHEKDRVHTYGSFYIA
                     DLTSDK"
     gene            3776..4111
                     /locus_tag="VFB80_17865"
     CDS             3776..4111
                     /locus_tag="VFB80_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZN35704.1"
                     /translation="MSAKRFILNSSRSSDQGKLINVGKESEAYKALTNTMTMLAADMA
                     EIGLAEGQMALIRTEFGEASFKCQAGKVPAGMIFVPYGPPTCRLMGGDTDGTGMPTSK
                     GWEVEVVPL"
     gene            4175..4333
                     /locus_tag="VFB80_17870"
     CDS             4175..4333
                     /locus_tag="VFB80_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35705.1"
                     /translation="MLRDANDEFAAGQSPHICIAGDDGEVAAWTEIEPGRFMPRRVRG
                     SYLGRALG"
     gene            4411..4758
                     /locus_tag="VFB80_17875"
     CDS             4411..4758
                     /locus_tag="VFB80_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35706.1"
                     /translation="MSLQIIENATCTFCGCVCDDINLHHDELRIHKAERACVLGTAWF
                     LNHTAEKKYPVALIGGKEAALDEAIEVAAGILHAADMPLIYGLSNTTCEAQKECAALA
                     DQLGGILDSHTSL"
BASE COUNT          942 a         1569 c         1607 g          869 t
ORIGIN      
        1 tttcggccgc gaatccctcg gtcgttttgg aactgacgga gctgctcaag aagattcgcg
       61 agggcggccg cacccggccc tagccgtcca ccaggaatcc aacgatgatt cgcctgcttg
      121 ccgccgcaat cgtgcccctg ctgcttgtgg ccggcgcctc cgcagccgat cgccccaacg
      181 tgctgatctt gctggccgac gacatgggct attccgacgc cggctgctat ggcggcgaag
      241 tgcagacgcc caacctcgac gtcctcgcga ccggcggcct gcggttcacg cagttctaca
      301 acacggcccg ctgctggccg acgcgcgggg cgctgctcac tggctattac gcccagcaaa
      361 tccgccggga caccgtgccc ggcatcgcta gcggcaacgc gggcacgcgg ccgaagtggg
      421 ccgtgctgct gccccagctg ctcaaaccgc tcggctatcg cagctaccac tccggcaagt
      481 ggcacgtgga cgggcagccg ctggccggcg gattcgaccg ctcgtacctg gtacaggacc
      541 aggaccgctt cttctcgccg cggaaccacg cggaagacgg cgtcgccctg ccgcagccgg
      601 cggcggacag cggctactac gccacgcggg cgatcgccga tcacgctctg aagtgcctcc
      661 gcgatcacgc cgagaagcac gcggaccagc cgttcttcca ctacctctgc ttcaccgcgc
      721 cgcactttcc gctgcacgcc ctgcccgagg acatcgccaa ataccgcggc cggttcgccg
      781 ccggctggga cgccatgcgg gtcgagcggc tggcccgcat gaagaagctg gggattgttt
      841 cgtgcgagct gtcgccgcgg accgccggcg tgccggcctg ggattcgctt tcggccgccg
      901 agcgggaggc gtggcagcgg cacatggaaa tccacgcggc gatgatcgac cgcgtcgatc
      961 aggagatcgg ccgcgtgctg gcgcagctcc gcgagatgaa ggtgctcgac aataccgtca
     1021 tcttctttct cagcgacaac ggggccagcg cggagaagct cgtccgcggc gacgggcacg
     1081 atccggccgc gccgcccggc tcggccaaga cctttatgtg cctagagccc ggctgggcga
     1141 acctggcgaa tgcgccgctc acgcgatcga agatcttcgt gcacgaggga ggcatctcga
     1201 cttcgctgat cgtccactgg ccggccggcg tcgccgcgcg cggcgagctg cgcaccaatc
     1261 caggccacgt gatcgatctc gtgccgacga ttctggaact gaccggcggc aagcgaccgg
     1321 agacaattga aggagagccc gtgccgccgt ctccgggcaa gagcctcgtg ccggtttttg
     1381 cgaaggacgg cagcgtcagc cgcgattacc tgtggtggta tcactcgggc aaccgggcga
     1441 tccgcgtcgg cgattggaag ctcgtgtcga ccggcgagga agggccgtgg gagctatacg
     1501 acctgcgcac ggaccgcagc gagtcgcacg acctggccgc gagccagccc gagaaggcgc
     1561 gcgagcttgc ggccgcctgg accgcgcgcc tggaagagtt ccgcgttctc gccgccaagg
     1621 atgctccgcc cgcgccggcg aagaaaaagg gcgctaaggc caagaagatg tagtcgccat
     1681 tcaggcgcgt ttgcggccaa cgaacaggta gtacggcacg cggctgagcg ggatatatgg
     1741 caccttggcc cggtgctcct ggaagtgcac gacgtcgaag tgccgctgca ggaacgggac
     1801 gtggtcgggc gaggggaaga cgttgtccat cgcaaaccag gccggccaga acgtccgtgt
     1861 catccagccg tgccgcgcga ggccgtcgct ggcgaacttg cgggagacgt aaaaatcgac
     1921 gacgccgatc gtgccgccgg gcttcagcat cgccaaggcg ttttcgagcg ccgcgaacca
     1981 gtcggggatc atcgtcagcg agtaggaaaa cgtcaccaca tcgaccggcc cgctcggcgg
     2041 ctgatacgtc gtcgcgtcgg cctccaccgt ttcgacattc gtccaaccct tcgccgcaat
     2101 ccgcttcttg gcgacctcca agagcgaatg cgacaggtcg agcacgtaaa ccttgccgag
     2161 gttatgaatc gaatcgccga agtagtcgag attcccgccc gttcccccac ccatatcgat
     2221 ccacgtgccg cctgccggtg gcggaatcaa attccagagc tcctgccggc ccttgagcaa
     2281 ccgcttgcgg aaatcgtcgt acgcttccgc ctggcccgaa tagaaattct ccatccgcgc
     2341 agcgtggtcc ttgccgcgga ccggtttcaa cgcgaggtga tagagaatct tcaggtcgga
     2401 ggcaaagctc atacaaatga caaatgacaa atgacacatg acgctcgacc gattcggtca
     2461 cttgtcactg tcatttgtca ctagtgagat ccgcaatgta aaagctccca tacgtatgca
     2521 cgcggtcctt ctcgtgcaac tccgccgcca gctgcgggtg gaatttcagc agctctggca
     2581 gcatccgcgg ctggccgtgg gtcgtgacgt tcacgcgatc gatgaagtcg gtcttcaggc
     2641 cgccgctgcg ccagatgatc cggctactgg tggcagcccg cttgaggatc gcctgccact
     2701 ccagctccaa gagcggaaag aacttatcgc tgagccagtc catgtggtcc agcagcacgt
     2761 atttcgaaat ctgcccgttg tgcttgtcga ggaagccctg caccgaatcg gtgtgcgtgc
     2821 tcacgcgatc ggccaggccg cccttgagct tgtggaagtt gtcctccttc aggtattccg
     2881 ggcagcagga gcggctgtac tcccccttca tgtagacccg ccagaagtaa ttgtcggcca
     2941 gcggcagctt gcagaagacg gcctcgacgg cgtcctgcac gaacttgacg atgccgccgt
     3001 ggtactgcga ctcgacttgc ttccgctggg ccttgggcac gccgaccatc gacagtgtcg
     3061 tgtcacgatt catcgcgaac cgcatcggcc gcgaccagaa cttttgcttg aggtgttttt
     3121 cgtaaatctc gcgctgctcg gcgagcgact tggcgtcgag cgcggcgtcg atgtgcggcc
     3181 ggacgcggat caagcggtcg gtgtagatct tgatcagccg ggcgaacgcc ccgctggtgc
     3241 cgcggaaata aaacggccgg gccttgttgt cgaagaactt gatccagcga tcccaccagg
     3301 cctgcgacca ggccggcagg gccggccgca tctggcgctg atagagccgg cgggcgtcgg
     3361 ggtgatagcc gtagccgaag agccggaaaa agtcctcgaa ctgcagcgac ttgatgccgg
     3421 ccagcttgag gtccagaagc gcgttctgcc gcgggttcat atcgacggca aagacgtgct
     3481 ccggcccgca gagggcatag tcaagagcgt tgcaacccgc ggaggtaatg actagcacgc
     3541 tgtcccgcgg ctgaatggcc aaggcgacgc ggtccagccg cggatcttcc cagcaggtat
     3601 tgtagaccag gttgtttccg tggacgaatt tgaagactcg tccactgacc caatcgaaga
     3661 cggacatttt cgcccccggg cgcgccaaac tgtgcatctt atcgccgtgg tattgcccaa
     3721 caagccgccg cgtcgtcgga ttgtcgatct ccgccatcgg aattagaatt cttggatgag
     3781 tgcgaaacga tttattctca attcctcccg ctcctcggat cagggaaagc tgatcaacgt
     3841 ggggaaggaa tcggaggcgt ataaggccct gacgaatacg atgaccatgc tggcggccga
     3901 catggccgag atcggcctgg ccgagggaca gatggccctg attcgcaccg agtttggcga
     3961 ggcctcgttc aagtgccagg cgggaaaggt ccccgccggg atgattttcg tcccctatgg
     4021 cccgccgacg tgcaggctga tgggcgggga taccgatgga acgggaatgc cgacttccaa
     4081 agggtgggaa gtggaggtcg tcccgctgta gcgatcggct cggcgggcgg gattaggata
     4141 gagattacga ttaggattag gaccggacaa aagaatgctg cgagacgcga acgacgaatt
     4201 tgctgcgggc cagagtccgc acatttgcat tgccggcgac gacggcgaag ttgcggcatg
     4261 gaccgagatt gaacctgggc ggttcatgcc gcgccgcgtc cgcggcagct accttggccg
     4321 ggccctgggc tgagtgttta ccgtgccgtc gtccggttcg gacgacaacg agtgtcaaga
     4381 aaaggcaaac tagcgtgtga ggaatcgatc gtgtctttgc agattatcga aaacgccacc
     4441 tgcaccttct gcggctgcgt gtgcgacgac atcaacctgc accacgatga gttgcggatt
     4501 cacaaggccg agcgggcctg cgttctgggc acggcctggt tcctcaatca cacggcggag
     4561 aaaaagtacc cagtcgcgct gatcggcggc aaagaggcgg cgctcgacga agcgatcgag
     4621 gtggcggcgg gcattctgca cgccgccgac atgccgctga tctatggcct gagcaacacg
     4681 acgtgcgagg cccaaaaaga gtgcgccgcc ctggcggacc aactcggcgg catcctggac
     4741 agtcacacgt cgttgtgaca cgggccaacc gagatcgccg cccagttggg tggcaaagta
     4801 acttgcacgt tgggcgaggt gaagcagaga gctgacttcc gccggcagca accaggtcga
     4861 gcttgccgtc ggcgttgacg tcgccgatga cggccgagtc ggcgctgtgc ggaagattca
     4921 gcgtgggctg cgcggtgaac gagcccaggc cgttcccctt ttgcacggac aggccccagc
     4981 cgctccg
//
LOCUS       DASRJB010000673         8106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3021513, whole genome shotgun sequence.
ACCESSION   DASRJB010000673 DASRJB010000000
VERSION     DASRJB010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021513"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFB80_17880"
     CDS             <1..276
                     /locus_tag="VFB80_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZN35707.1"
                     /translation="IAAFDRQWIDWLLDNLARGTVRFAKWWEFIADQSLVDGLVNLVA
                     GWTWSLGLSLRAIQTGRIRQYVMFIVIGAIAIFVLISFFWSPTLARP"
     gene            318..2105
                     /locus_tag="VFB80_17885"
     CDS             318..2105
                     /locus_tag="VFB80_17885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZN35708.1"
                     /translation="MTPVLLLSAIVFLPAVAALGIALIPSDQKDAIRWATLIATAAVM
                     GLVAAFLLFPYDPKISFDAAQAGMQDAFNYAWIPSFRIEYFMGLDGISFPLVVLTAFI
                     SLLAMGASWSIEKHVKAYCILFLLLETGMLGVFVALDFFLFYIFWEVMLLPMYFLIGV
                     WGGPRKEYAAIKFFLYTLAGSVLMLIAILMLYFSSDLRGLTDAQLVASHVIKDTAGLT
                     PEAAEDNANALKEIRESTSPVHTFNIVALQQIGQHTKQLDAVQWWSFVLLFIGFAIKV
                     PSVPVHTWLPDAHVEAPTPISMILAGILLKMGGYGILRICYPICPAAGYDLSWWVCLL
                     GIVSMVYGALAAMAQTDFKRLVAYSSVSHMGYVVLGMGVWSATAGNEFNSYYWDMGVK
                     GAMFQMIAHGISSAGMFFMVGIIYDRVHHRNLNEFGGLFGKMPVYTAMAIILFFAGLG
                     LPGLCGFIGEVFVVLSVWKFKYWMAVVAAAVVILTAGYILWAVQRVYLGPEYKGPHGD
                     HLTPSNFRENLIGATLCVFAVIFGIFPYQLPGGQPSVLRLMDETIDKQVSDLATWTKA
                     NRAAGTPASAPAMPASPTTPASAPAATEL"
     gene            2127..3935
                     /locus_tag="VFB80_17890"
     CDS             2127..3935
                     /locus_tag="VFB80_17890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZN35709.1"
                     /translation="MNLHDLVSELIIDTKGAAGALFAPATADSSLRAFLPELIICATV
                     LLLLAVRLPKWGRYIDTFWIALAGSAAALYYAAPWLHLGSMLADASQPATVTRMEIFT
                     GMLVYDTFSVYVRSLLFAFAILFVIFTRLSGIPDREDGQDVYSLVLGATLGMCLMASA
                     NHLLTVFLAVEMASVPSYVLAGLLKGRRVASEAALKYSVYGAGAAGVMLYGISLIAGL
                     LNTAHLPTIAATLAGRIAADNLSGSELMVLAMGGLFISVGLAFKLSAVPFHFWCPDVF
                     EGATAEVDAFLSIASKAAALALLVRVALGVSTLPPEGFAPGQRTAAIQAKAGAAAQSA
                     ALFNVADEPAAKPVTAVAAAQTPTEALAPVRTFTAKLIAFFAIITCTFGNLAAYGQNN
                     LKRLLAYSTIAHAGYMMMPVAAAVAMAGINKAGAEQAIAALAIYMAVYLFMNLAAFAV
                     VAFLRNAMRSEDLADYAGLVRRSPLTVVCFAVTLFGLIGLPPLSGFIGKFAIFAALVD
                     GWQSLEAAGISGRFLLILLIVGGINTAVCLFYYLRVAKVMTIDEEPRDRPPHSFSDVS
                     LGGAYLWLITLPTALLIVSWDWLSAVTLAAARNLLS"
     gene            3966..4400
                     /locus_tag="VFB80_17895"
     CDS             3966..4400
                     /locus_tag="VFB80_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35710.1"
                     /translation="MLMSPPSTTDVLNRILVLHERSLSMYLSYAPPADLQASERAKAV
                     LGQIVEDQRRTIDRLATLVLDGSGTVDYGEFPLSFTSLHDLSLKYLLNLLIDRQRKLV
                     AVCERLAEQLNLAPFAQAAARESVGEAKGHLDNLLELQKSLA"
     gene            4453..5259
                     /locus_tag="VFB80_17900"
     CDS             4453..5259
                     /locus_tag="VFB80_17900"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZN35711.1"
                     /translation="MRFDLPRLAASNLIARIDFHETLASTSDRALALAAEGAAQLPLL
                     VLAERQTAGRGRGANRWWSADGALTFSLVLEAPPEQLPPDRWPQVALAAGLAVCEALQ
                     SLAPAAPMQVKWPNDVYLAGRKVAGILSESVPGWRDRLVVGMGVNVNNWVQGSGFGIQ
                     QAEAAALAQIATALIEHDGLPRDLTAVLLAVLDQFDRRWGELLGGSFAPLAKVFRERC
                     FLTGKTVTIEQPGGQALAGRCRGIDDEGALLVQSLAGEHRVRSGTVIRWE"
     gene            5284..5850
                     /locus_tag="VFB80_17905"
     CDS             5284..5850
                     /locus_tag="VFB80_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35712.1"
                     /translation="MKVIAVLCGLLFASSSAAIILAAIHIESIVITGPTAGFLGFLLA
                     LVAAFRRRRLLTAANLSLPLLGVTVFLLIFCNRWSPRDAQHPVTAILLGYEVLAAPLW
                     LGGLWSIVHGSPAGPRKWQFPIRTLMIVTLVVALALGAARIAMRQSAEVLVAVAAGLL
                     ILVLCALVALAVQALNAQSPPLKSPSPA"
     gene            5942..>8106
                     /locus_tag="VFB80_17910"
     CDS             5942..>8106
                     /locus_tag="VFB80_17910"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35713.1"
                     /translation="MARLIPCLLVACLPLAALAADPDEQLFKEPINVNVPHIKSDKTV
                     KYDYDIVYVRADRAGDTVHKRYYTDFSQPVTMEPGADLMLLHPDGTEEVLVEGGNGSV
                     TDPMISFDGQWVFYSYLYDLRNRSQWDPPAAGADIFKLHLTTRKIVKLTTQEFHPNTG
                     AAPWSSDYRKGEEGTTHFHYGVFNMGPCPLPGGRLVFTSNREGYRPPKGYPVIALQLF
                     VMNDDGTNVEKIGHLNIAGALHPVVLKDGRIMFSSLESQGIRSDISWGIWTIHPDGTN
                     WNPLVSAFDPGGASNGFHFQSQLSDGSLIVEEYYNQNNSGFGAYVRLPPSPPDGYPAF
                     GPARMNDPRNPPWRFGRFDNGRPKNYRLPFMPSGSVSFTPFSLNQEGPADTAILGDKD
                     SPKVGKFTHPSGAPDNHLLTIYSPGPVNHQYTFLPQLDGGIYLAKDGKIIEQPGDMLL
                     IKNDPNYNESWPRAVVPYERIYGVKEPRRIKPLANDGSLSPHLPAGTPFGLVGTSSFY
                     KRESYPNGRVAEGQVTAEFGGGNDPWKDLDAFTSHGNGMPVAWHNQGGDVGLYSNDEI
                     HAVRILAMEPTTDRQRGAHSGRRFVSHARERLRILGEIPLRKFSGDQQPPDPDGHPDT
                     SFLAKIPADTAFTFQTIDKRGLMLNMAQTWHQLRPGEIRHDCGGCHAHSQKPTDFALT
                     AAAKSNYKLWDLTKETPLVLNRRDHAASPGGNAGAAESGV"
BASE COUNT         1283 a         2766 c         2558 g         1499 t
ORIGIN      
        1 atcgcggcgt tcgacaggca gtggatcgac tggctgctgg acaatctggc ccgcggcacc
       61 gtccgctttg ccaagtggtg ggagttcatc gccgatcagt cgctagtgga cggcctggtg
      121 aacctggtcg ccggctggac ctggtcgctg ggcctgtcgt tgcgggcgat tcagaccggc
      181 cgcatccgcc agtatgtgat gttcatcgtg atcggcgcga tcgcgatttt cgtgctgatc
      241 agcttcttct ggagcccgac gctcgcccgc ccctaaccaa ctgcacccaa cgaccaaccg
      301 ctcccggata acgaacgatg acgcccgtcc tgctcctcag tgccattgtc tttctgccgg
      361 ccgtggccgc gctgggcatc gccctgatcc ccagcgatca gaaagacgcg atccgctggg
      421 ccaccttgat cgccaccgcc gcggtgatgg gcctcgtggc cgcattcctg ctgtttccct
      481 acgacccgaa gatcagcttc gacgcggccc aggccggcat gcaagacgcc ttcaactacg
      541 cctggattcc gtcgtttcgc atcgagtact tcatgggcct ggacgggatc agcttcccgc
      601 tggtcgtgct gacggccttc atcagcctgc tggcgatggg ggcgagctgg tcgatcgaaa
      661 aacacgtcaa ggcctactgc atcctgttcc tgctgctcga gaccgggatg cttggcgtct
      721 tcgtggcgct cgacttcttc ctgttctaca ttttctggga agtgatgctc ctgccgatgt
      781 actttctgat cggcgtctgg ggcggcccgc gaaaggaata cgccgcgatc aagttcttct
      841 tgtacacgct ggccggcagc gtgctgatgc tgatcgcgat cttgatgctc tatttcagca
      901 gcgacctgcg gggactgacc gacgcgcagc tggttgcctc gcacgtcatc aaggacacgg
      961 cgggcctgac gccggaagcg gcggaggaca acgcgaatgc cctgaaggag atccgcgaaa
     1021 gcacctcgcc cgtccacacg ttcaacatcg tagccctcca gcagatcggc cagcacacga
     1081 aacagctcga cgccgtgcaa tggtggtcgt tcgtgctgct gttcattggc ttcgcgatta
     1141 aggtccccag cgtgccggtg cacacctggc tgcccgacgc ccacgtcgaa gcccctacgc
     1201 cgatctcaat gatcctggcg ggcatcctgc tgaagatggg cggctacggc atcctgcgaa
     1261 tctgctaccc gatctgtccc gcggccggct atgacctgtc ctggtgggtc tgcctgctgg
     1321 gcatcgtgag catggtctac ggcgcgctcg cggcgatggc ccagacggac ttcaagcggc
     1381 tggtcgccta cagctcggtc agccacatgg gctacgtcgt actcggcatg ggggtctggt
     1441 cggcgaccgc cggcaacgag ttcaactcgt actattggga catgggcgtc aaaggcgcga
     1501 tgttccagat gatcgcccac ggcatcagct cggccggcat gttcttcatg gttggcatca
     1561 tctatgaccg ggttcaccac cgcaatctga acgagttcgg cggcctgttc ggcaagatgc
     1621 ccgtctatac cgcgatggct atcatcctgt tcttcgccgg cctcggattg ccgggcctgt
     1681 gcgggttcat cggcgaggtg ttcgtcgtcc tctccgtctg gaagttcaag tactggatgg
     1741 cggttgtggc cgcggcggtg gtgatcctca ccgccggcta catcctgtgg gccgtgcagc
     1801 gggtctatct cggacccgaa tacaagggcc cgcacggcga ccacctcacg cccagcaatt
     1861 tccgcgagaa cctgatcggc gcaacgctct gcgtcttcgc cgtgattttt ggcatcttcc
     1921 cgtaccagtt gccgggcggg caaccctcgg tgctgcggct catggacgag acgatcgaca
     1981 agcaggtgag cgacctggcc acgtggacca aagccaaccg ggctgccggc acgccggcgt
     2041 ctgctccagc catgccggca tcacccacca cgccggccag cgcccccgcc gccactgagc
     2101 tgtaacgttt gccataaagc actgccgtga acctgcacga cctagtcagc gagctgatca
     2161 tcgacaccaa gggcgctgcc ggagcgctct tcgcgccggc caccgccgac tcctcgctcc
     2221 gcgcgttcct gcccgagctg atcatctgtg cgaccgtgct gctgttgctc gccgtgcgcc
     2281 ttccgaagtg gggccggtac atcgacacgt tctggatcgc cttggcaggc tcggccgcgg
     2341 ccctctacta cgcggccccc tggctgcacc tgggctccat gctggccgac gcttcgcagc
     2401 cggcgacggt cacgcggatg gagatcttca ccgggatgct ggtgtacgac acattcagcg
     2461 tctatgtccg gtcgctgctg ttcgcttttg cgatcctgtt cgtgatcttc acgcggctca
     2521 gcggcatccc cgaccgcgag gacggccagg acgtctattc gctcgtgctg ggcgcgacgc
     2581 tcggcatgtg cctgatggcc tcggccaacc acctgttgac ggtcttcctc gcggtcgaga
     2641 tggcgagtgt gccgtcgtac gttttggcgg ggctcttgaa gggccgccga gtcgccagcg
     2701 aggccgcgct gaagtactcg gtgtacgggg ccggcgcggc gggcgtaatg ctctatggca
     2761 tcagcctgat cgcgggcctc ttgaacacgg cccacctgcc cacgatcgcc gccacgctcg
     2821 ccgggcggat cgcggcagac aatctctccg gcagcgagct gatggtgctc gccatgggcg
     2881 gtctgttcat ttccgtcggc ctcgccttca agctctcggc ggtcccgttc cacttctggt
     2941 gcccggacgt gttcgagggg gcgacggccg aggtcgatgc cttcctcagc attgccagca
     3001 aggcggcggc gctggcactg ctggtgcgcg tggcgctcgg cgtcagcacg ctgccgccgg
     3061 aaggtttcgc accgggacag cgtacggcag ccatacaggc caaggcagga gccgcggcac
     3121 agtcggccgc gctgttcaac gtggccgacg agcccgccgc gaagccagtg accgcggtgg
     3181 cggccgccca aacgccgacc gaggcgctgg cgcccgtccg cacgttcacg gccaagctga
     3241 tcgccttttt cgccatcatc acttgcacct tcggcaacct ggcggcctac ggccagaaca
     3301 acctgaagcg gctgctggcc tattcgacga tcgcccacgc cggctacatg atgatgccgg
     3361 tcgcggccgc cgtggcgatg gccggaatca acaaggccgg tgctgagcag gcgattgccg
     3421 cgctggcgat ctacatggcc gtctacctgt tcatgaatct ggctgcattc gccgtcgtgg
     3481 ccttcctgcg gaacgcgatg cggagcgagg acctggccga ctacgcgggc ctggtccgcc
     3541 gctcgccgct gaccgtggtc tgcttcgccg tgacgctgtt tggcctgatc ggcctgccgc
     3601 cgctgtcggg cttcatcggc aagttcgcga tcttcgccgc cctggtcgat ggctggcagt
     3661 cgctcgaggc ggccggcatc agcggccggt tcctgctgat cctgctcatc gtcggcggca
     3721 tcaacacggc ggtttgcctg ttttattacc tgcgggtcgc caaggtgatg acgatcgacg
     3781 aagagccgcg ggaccgcccg ccccactcgt tctcggacgt ctcgctcggc ggggcctact
     3841 tgtggctgat cacgctgccc acggccctgc tcatcgtcag ctgggactgg ctcagcgccg
     3901 tgacgctcgc cgccgcccgc aacctgctct cctgaaccgg ccgcatccca gacagcccgc
     3961 ccgccatgct catgagcccg ccgagcacga ccgacgtcct gaaccgcatc ctcgtgctcc
     4021 acgagcgctc gctgtcgatg tatttgagct acgccccgcc ggccgatctg caggccagcg
     4081 agcgtgccaa ggcggtgctc gggcagatcg tcgaagatca gcggcggacc atcgatcgcc
     4141 tggccacgct cgtgctcgac ggcagcggca cggtcgatta cggcgagttt cccctgtcgt
     4201 tcacgtcgct ccacgatctg tcgctgaagt acctgctcaa cctgctcatc gaccggcagc
     4261 ggaagctggt cgccgtttgc gagcggctgg ccgaacagct gaacctggcc cccttcgccc
     4321 aggccgccgc ccgcgaatcc gtcggcgagg ccaaggggca tctggacaat ctgctggaac
     4381 tgcaaaagtc gctggcctga acgcggcttt cgccaaagcc ggatcgagca cgtttcgctg
     4441 gatcgactcc gcatgagatt cgatctcccc cgcctcgccg ccagcaacct tatcgcgcga
     4501 attgactttc acgagacgct cgcctccacg agcgaccggg cgctggccct ggccgccgaa
     4561 ggcgcggccc agctgcccct cctggtgctg gccgagcggc agacggccgg ccgcggccgc
     4621 ggcgcgaatc gctggtggtc cgccgacggc gcgctcacgt tctcgctcgt gctcgaggcc
     4681 ccgcccgagc aactgccgcc cgaccgctgg ccgcaggtcg cgctggccgc gggcctggcc
     4741 gtttgcgagg ccctccagtc gctcgcgccc gctgccccga tgcaggtgaa atggcccaac
     4801 gacgtctacc tcgcgggccg gaaggtcgcc ggcatcctct cggagtccgt tcccggctgg
     4861 cgcgaccggc tggtcgtcgg gatgggggtg aacgtaaaca attgggttca gggttcaggg
     4921 ttcgggattc agcaggcgga ggcagcggcc cttgcacaga ttgcgactgc gctcatcgaa
     4981 cacgatgggc tgccgcgaga tttgaccgcg gtgctgctgg cggtgttgga tcagttcgac
     5041 cggcggtggg gggagttgct gggtggctcg ttcgccccat tggccaaggt ctttcgcgag
     5101 cggtgcttct tgacgggcaa gaccgtgacg atcgagcagc cgggcggcca ggcgctcgcc
     5161 ggccgctgcc gcggcatcga cgacgaaggc gcactgctgg tgcagagcct ggccggcgag
     5221 catcgcgtgc gctcggggac ggtgatacgc tgggagtgag ttccgaccgt tgcgagaggt
     5281 ccgatgaaag tcattgccgt tctctgcggc ctgctgttcg cctccagcag cgctgcgatc
     5341 atcctcgcgg cgatccacat tgagtcgatc gtcattaccg gacctacggc cggctttctc
     5401 gggtttttgc tggcgctggt cgcggccttt cgccgccggc ggctgttaac cgcggcgaac
     5461 ctgtcgctgc ccctgctggg cgtgaccgtt tttctgctga tcttttgcaa tcgctggtcc
     5521 cccagagacg cgcagcatcc ggtgacggca atcctgctcg gctacgaggt gctcgccgcg
     5581 ccgctttggc tgggcgggct gtggtccatc gtccatggct cacccgcggg tccgcgcaag
     5641 tggcagtttc ccatcaggac tctcatgatc gttacgcttg tcgttgcgct tgcgctgggg
     5701 gcagcgcgga tcgccatgcg gcagtcggcg gaggtgctcg tcgcagttgc cgccggcttg
     5761 ctcatcctcg tgctctgcgc gctcgtggcg ctggccgtgc aggctctgaa tgcccagtcg
     5821 ccgccgctga agtcgccgtc gccggcttga agttgatccg ccactgggcc tctgcgaaaa
     5881 tgggcctctg cgaaagtgca gcggctcgct cccaccactc gcctctgcgg gaacccctgt
     5941 aatggcacgc ctgatcccct gcttgctcgt tgcctgcttg ccgctggccg ccctcgccgc
     6001 cgatcccgat gagcaactct tcaaggaacc gattaacgtc aacgttccgc acatcaagag
     6061 cgacaagacg gtcaagtacg actacgacat cgtctacgtc cgggccgatc gggcgggcga
     6121 cacggtccac aagcggtact acaccgattt ttcgcagcca gtgacgatgg agccgggcgc
     6181 cgacctgatg ctgctgcacc ccgacggcac cgaagaggtg ctggtcgaag gaggaaacgg
     6241 ctcggtcacc gatccgatga tcagcttcga cggccagtgg gtcttctatt cgtacctcta
     6301 tgacctgcgg aatcgcagcc agtgggatcc gccggcggcc ggggcggaca tcttcaagct
     6361 gcacctcacg acgcggaaga tcgtcaagct cacgacgcag gagttccacc ccaacaccgg
     6421 cgccgctccc tggtcgagcg attatcgcaa aggcgaagag ggcacgacgc acttccacta
     6481 cggtgtcttc aacatgggtc cgtgcccgct gcccggcggg aggctcgtct tcaccagcaa
     6541 ccgcgagggc tatcgcccgc cgaagggcta tccggtcatt gccctgcagc tgttcgtgat
     6601 gaacgacgac gggacgaacg tcgagaagat cggccacctc aatatcgccg gcgctttgca
     6661 tccggtcgtg ctcaaggatg ggcggatcat gttcagctcg ctcgagtcgc agggcatccg
     6721 cagcgacatc tcctggggca tctggacgat ccatcccgac ggcacgaact ggaacccgct
     6781 ggtcagcgcg ttcgatcccg gcggggccag caacggcttt cacttccagt cgcagctctc
     6841 cgacggctcg ctgatcgtcg aggaatatta caaccagaac aacagcggct tcggggccta
     6901 cgtccggctg ccgccctcgc cgccggacgg ttacccggcg ttcggcccgg cgcggatgaa
     6961 cgacccgcgc aacccgccct ggcggttcgg tcgcttcgac aacggccggc cgaagaacta
     7021 ccggctgccg ttcatgccca gcggctccgt gtcgttcacg ccgttttcgc tcaaccagga
     7081 aggccccgcc gacacggcga tcctcggcga caaggattcg cccaaggtcg gcaagtttac
     7141 gcatcccagc ggcgcgccgg acaatcacct gctcacgatc tactcgcccg ggccggtcaa
     7201 ccatcaatac acgttcctgc cgcagctcga tggcggcatc tacctggcca aggatggcaa
     7261 gatcatcgag cagccgggtg acatgctgct aatcaagaac gacccgaact acaacgagtc
     7321 gtggccgcgg gcggtcgtgc cttacgagcg gatttacggc gtgaaagagc cgcggcggat
     7381 caagccgctg gccaacgacg gctcgctctc gccgcatctg cccgcgggca cgccgtttgg
     7441 cctcgtcggc acgtccagct tctacaagcg cgagagctat cccaacggca gggtcgccga
     7501 gggccaggtg actgccgagt tcggcggcgg caacgatccc tggaaggacc tcgatgcctt
     7561 caccagccat ggcaacggca tgccggtcgc ctggcacaac cagggcggcg acgtcggcct
     7621 ctattccaac gacgagatcc acgccgtccg catcctggcg atggagccca ccaccgaccg
     7681 ccagcgcggc gcacattcgg gccggcgatt cgtcagccac gcccgcgagc ggctgcgcat
     7741 tctcggcgag attccgctgc gcaagttctc cggagaccag cagccgccgg atcccgacgg
     7801 ccatcccgac acgagctttc tggccaagat ccccgccgac acggcgttca cgtttcagac
     7861 catcgacaag cgcgggctga tgctcaatat ggcccagacc tggcatcaac tgcggccggg
     7921 cgagattcgc cacgactgcg gcggctgcca cgcccatagc cagaagccga cggattttgc
     7981 gctcacagcc gcggccaagt cgaattacaa gctgtgggat ctgacaaagg aaacgccgct
     8041 ggtgctcaac cgccgcgacc acgcggcgag tcctggcggg aacgccgggg ccgcagagtc
     8101 cggcgt
//
LOCUS       DASRJB010000674         6896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_198038, whole genome shotgun sequence.
ACCESSION   DASRJB010000674 DASRJB010000000
VERSION     DASRJB010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198038"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFB80_17915"
     CDS             complement(<1..245)
                     /locus_tag="VFB80_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35714.1"
                     /translation="MPPTALASPSQLRPDEKPLAEYRAVSRAAVAAVGLGLASALVLI
                     TPLLGFVAVAAAVVAVLALRSIAASNGQLVGRLPAIVG"
     gene            complement(294..962)
                     /locus_tag="VFB80_17920"
     CDS             complement(294..962)
                     /locus_tag="VFB80_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35715.1"
                     /translation="MMFLALFVVFACVAAAIWFQGFWNGIVAFMNILLAAMIATNLWE
                     PLTTMVENFGAASWTYMLDFVLLWFLFFAVFAVLRGITDLLSRTQVKFELPLEMAGRS
                     VMALLCGWLMVCFVAFTLQMAPLNSAEPLGAWSSPNSKSFLVAAPERLWMRFMFDRSR
                     GAFARGHYSDRPQHPSDQSLNVEAFDPLSEFALKYRQRRDEFQKQTDMRVGSAPEPAA
                     AGQP"
     gene            complement(1078..2892)
                     /locus_tag="VFB80_17925"
     CDS             complement(1078..2892)
                     /locus_tag="VFB80_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN35716.1"
                     /translation="MLRTASWMSSGTLLTAALLVLSAGGQLAAQDAWPAYTAPFQRGP
                     GFYLAFWKIFALLFIVWMWVKSADWVSRDSRELGEAIGLPSRIWNPVMVFSFLIGFLA
                     AVTIPIFFAGGLVALLAYAGPFIAYVAIRNGKVTDDQKVFTPSHLKNWFANLGKRTRK
                     ARDAQHAWQMGPQVDISAVGPLQMENQQALIEARQSPAYVSVKYLLADALSQRTERIM
                     LDYTAEGVGVNYLIDGVWHNAAPKVHEKQQLDRQLGDQMLAVLKRVCHLKMEERRARQ
                     DGKLKLEFGGTKYDATFSSQGTQTGERAVLQFIPITKHVRSLEELGMRDKLREQLPEL
                     IGPGHHGLVIFAAMPGDGLTATWQAALRSTDRLMRDFIAVEDIHKREPDVENVDCSKY
                     DSAKGETPEGVIPKLFLKLPEVICIPEIASTEALKLLLKYIVTEDRLGIISLKAKDAA
                     DALLRVLALKVPANEVAPILKGVVYTRLVRRLCETCREAYQPEPALLQKLGIPQGRVQ
                     VLYREKQPPQPGQEKKKGEPEICPDCRGIGYKGRIALYEFLVVDDKVKEALVKDPKAE
                     TIKKLARAAGNRSLQDEGILLVALGTTSITELQRALKQ"
     gene            complement(2892..4007)
                     /locus_tag="VFB80_17930"
     CDS             complement(2892..4007)
                     /locus_tag="VFB80_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZN35717.1"
                     /translation="MSVEMLDVEVNKFFRALVKLEGSDLHMKVGKPPCVRVRNELKPL
                     NHPVIERREMAEYLVPMMGERSRRIFDEEGGADFSHTCDVDGVKWRFRVNILQQSGCM
                     GMVARRISIKIPNFEGLYLPPSIEGLCNYDQGMILLAGVTGSGKSTTIASMLDYINHR
                     ERVHILTLEDPIEFIFTEDKALINQREVGFDVKDFKIGMKHAVREDPDIMLVGEMRDE
                     ETFMTAIHAAETGHLVFGTIHASSSPSTIGRILDLFPEAMHKAIRSAIAFNMKGIVAQ
                     KLLKSIKPGVPRVPTCEIMTFTTMIKKLVLEAQDNKLADAIRIGAADGMQDFTMSLKQ
                     LIDDQLIDRPTAFEVAPNPDALKMALKGINVAQPGII"
     gene            complement(4136..4993)
                     /gene="hisF"
                     /locus_tag="VFB80_17935"
     CDS             complement(4136..4993)
                     /gene="hisF"
                     /locus_tag="VFB80_17935"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZN35718.1"
                     /translation="MLAKRVIPCLDVDRGRVVKGTNFLNLRDAGDPVQVAARYEQEGA
                     DELVFLDITASHEGRDIMLDVVRRTAEQVFMPLTVGGGIRTIDDIRALLNAGADKVSI
                     NSAACKNPQFVAEAARRFGSQCIVVNIDPKRVRGQESGASGQEPVARSQGPVGQESGS
                     TTHHSPLTTHSFEVFINGGRVPTGLEAVSWARRVEELGAGEIVLTSMDCDGTKDGYDL
                     EITSSVSRAVTIPVVASGGAGRPEHLADAILIGRADAALAASIFHFGEYTIRQTKEIM
                     AARGIPVRL"
     gene            5563..6879
                     /locus_tag="VFB80_17940"
     CDS             5563..6879
                     /locus_tag="VFB80_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35719.1"
                     /translation="MKHLLGILIAAAAGAAGCAGGAWLEGPSDADLPIQPPARGYPTA
                     LRPVAASISDEAATPQSAPAGPAPSTAVLPLDPNPPPAAPPKHSQLIDPRPPAPALAV
                     VPPQYQRPPAPTAPALQALPPATPIASSLPSAAAPPQATVEPGLAPIVSASSVRAAAA
                     SSADRQPTPLQPPLAAAAVAPAATSGSVPSSEERRAEIERLRKEFTAALEADIRERQA
                     RNAKDEELPRLEQQLRLAYLLADRLDDAVAAVDSLDPPQQEAYKNLMFGLGVWLSPDE
                     AHRTPLRSAKVLKSLREATADLSMASKLELRNLAFCEKVDSYGWYTEFPRSEFAPKQQ
                     VILYVEVENFAAEHKSAAGYETELQGRYEILDASGQIVAERELPLDKEVCRNHRRDYY
                     LWYRIYLPDEIAPGKYRLELSIEDRKAGAKYQGRKFGEGMIEFAIR"
BASE COUNT         1207 a         2215 c         2235 g         1239 t
ORIGIN      
        1 ccgacgatcg cgggcagccg gccgacgagt tgcccgttcg acgcggcgat cgaccgcagc
       61 gccaggaccg ccacgaccgc cgccgccacg gcgacgaacc ccaaaagcgg tgtgatcagg
      121 acgagtgccg acgccaggcc caggccgaca gccgccacag ccgcccggct gaccgcgcgg
      181 tattcggcca gcggcttctc gtcgggccgc aactggctcg ggctggcgag ggcggtgggg
      241 ggcatggcaa gacggctgtt gtacggtcca ggtccgccgc gggttcgcgc cgcctatggc
      301 tggcccgcag ccgccggctc gggcgccgag ccgacccgca tgtcggtctg cttttggaac
      361 tcgtcgcgcc gctggcggta tttcagggcg aattcggaga gcgggtcgaa cgcctccacg
      421 ttcaggctct ggtcggaggg atgttgcggc cggtcgctat agtggccgcg ggcgaacgcg
      481 ccccgcgagc ggtcgaacat gaaccgcatc cagagccgct cgggcgcggc gacgaggaac
      541 gacttggagt tggggctcga ccacgcgccc agcggctcgg ccgagttgag cggcgccatc
      601 tggagcgtga acgccacgaa gcagaccatc aaccagccgc agagcaaggc catgaccgac
      661 cggccggcca tctccagcgg cagctcgaac ttgacctgcg tgcggctgag caggtcggtg
      721 atcccgcgga ggacggcaaa gacggcgaag aacagaaacc agaggagcac gaagtcgagc
      781 atgtaggtcc acgaggccgc gccgaagttt tcgaccatcg tcgtcagcgg ctcccacagg
      841 ttcgtggcga tcatcgccgc cagcaggatg ttcatgaagg ccacgatgcc gttccagaac
      901 ccctgaaacc agatcgccgc cgcgacgcag gcaaagacga cgaagagtgc gaggaacatc
      961 atgggaggcg gtcagtcggt tggtcggtgg tcggttgatc ggtcagtcgg tggtcggttg
     1021 atcggtcagt cggtcaggcg ctctaccgac taaccgaccc actgaccacc gactgaccta
     1081 ctgtttcaag gcgcgctgta gttcggtaat ggacgtcgta cccaacgcga ccagcaaaat
     1141 gccttcatcc tggaggctgc ggttgccggc ggcgcgggcg agttttttga tcgtctcggc
     1201 cttcgggtct ttgacgaggg cctctttgac cttgtcatcc accacgagga actcgtagag
     1261 ggcgatccgc cccttgtagc cgatgccccg gcagtcgggg caaatctcgg gctcgccctt
     1321 cttcttttcc tggccgggct gcggcggctg cttttcgcga tagagcacct gcacgcggcc
     1381 ctgcgggatg cccagcttct gcagcagggc cggctcgggc tgataggcct cgcggcacgt
     1441 ctcgcacagc cggcggacca gccgcgtata gacgacgccc ttgaggatcg gcgcgacctc
     1501 attggccggc accttcaggg ccagcacccg cagcagtgcg tcggcggcat ccttggcctt
     1561 caggctgatg atgcccagac ggtcctcggt gacgatgtac ttgaggagca gcttgagggc
     1621 ctcggtgctg gcgatctccg ggatgcaaat cacctccggg agcttgagga acagcttagg
     1681 aatgacgccc tcgggcgtct cgcccttggc cgagtcgtat ttggagcaat caacgttttc
     1741 gacgtcgggc tcgcgcttgt ggatgtcctc gacggcgata aagtcgcgca tcaggcggtc
     1801 ggtcgagcgg agggcggcct gccaggtcgc ggtcaagccg tcgcccggca tcgcggcaaa
     1861 gatgaccagg ccgtgatgac ccggcccgat caactccggc aattgctcgc ggagcttgtc
     1921 gcgcatcccc agctcttcca gcgagcggac gtgcttggta atcggaatga actggaggac
     1981 ggcccgctcg ccggtctgcg tgccctgcga actgaaggta gcgtcgtact tcgtcccgcc
     2041 aaattcgagc ttgagcttgc cgtcctgccg ggcccgccgc tcttccattt tgaggtggca
     2101 gacgcgcttc agcaccgcca gcatctggtc gccgagctgg cggtccagct gctgcttctc
     2161 gtgcaccttc ggcgcagcgt tgtgccagac gccgtcgatc agataattca cgccgacccc
     2221 ttcggcggtg taatccagca tgatccgctc ggtccgctgg ctcagggcgt cggccagcag
     2281 gtatttcacg gagacatacg ccggcgactg gcgggcctcg atcagggcct gctggttctc
     2341 catctgcagt gggccgacgg cggagatgtc gacctgcggg cccatttgcc aggcgtgctg
     2401 ggcatcgcgg gccttgcgcg tccgcttgcc gagattggcg aaccagtttt tcaggtgcga
     2461 cggcgtgaag accttctggt cgtcagtgac tttgccgttg cggatggcaa cgtaggcaat
     2521 gaacggcccg gcataggcga gcagcgccac gagcccgccg gcaaaaaaga tgggaatcgt
     2581 caccgcggcc aggaagccga tcaagaacga aaacaccatc accgggttcc agatccggga
     2641 cggaaggccg atcgcctcac ccagctcccg gctgtcgcgg ctgacccagt cggctgattt
     2701 cacccacatc cagacgatga acaagagggc gaaaatcttc cagaaggcca ggtagaagcc
     2761 cggcccgcgc tgaaagggcg cggtgtaggc gggccacgcg tcctgggccg ccagctggcc
     2821 gccggcggaa agcacgagga gcgcggcggt gagcagcgtc ccgctgctca tccaagaagc
     2881 agtgcgaagc atcagatgat cccgggctgc gccacattga tccctttgag cgccatcttg
     2941 agggcgtcgg gattcggtgc aacttcaaag gcggtgggtc ggtcgatgag ctggtcgtcg
     3001 atcagctgct tgaggctcat ggtgaaatcc tgcatgccgt cggccgcgcc aatgcggatc
     3061 gcgtcagcca gcttgttgtc ctgggcctcc agcacgagct tcttgatcat cgtcgtgaag
     3121 gtcatgatct cgcaggtcgg cacgcggggc acgcccggct tgatgctctt gagcagcttc
     3181 tgggcgacga tccccttcat gttgaaggcg atcgcgctgc ggatggcctt gtgcatcgcc
     3241 tcggggaaca ggtccaggat gcggccgata gtcgaagggg agctggaggc gtggatcgtg
     3301 ccgaacacga ggtggccggt ctcggccgcg tgaatggccg tcatgaacgt ctcttcgtcg
     3361 cgcatttcgc cgacgagcat gatgtccgga tcttcgcgga ccgcgtgctt catgccgatc
     3421 ttgaagtcct tgacgtcgaa gccgacttcg cgctggttaa tcagcgcctt gtcctcggtg
     3481 aagatgaact cgatcgggtc ttccagcgtc agaatgtgca cccgctcgcg gtggttgatg
     3541 tagtcgagca tcgaggcgat ggtcgtgctc ttgccggagc cggtcactcc tgccagcagg
     3601 atcatcccct ggtcgtaatt gcacaggccc tcgatcgacg gcggcagata cagcccctcg
     3661 aagttcggaa tcttgatgct gatgcggcgg gcgaccatgc ccatgcagcc gctctgctgc
     3721 aggatgttga cgcggaaccg ccacttgacg ccgtccacgt cgcaggtgtg cgagaagtcg
     3781 gccccacctt cttcgtcgaa aatccgccgg ctccgctcgc ccatcatcgg cacgagatac
     3841 tcggccatct cgcggcgctc gatcaccggg tggttgagcg gcttgagctc gttgcggacc
     3901 cgcacgcagg gcggcttgcc aaccttcatg tgcaagtcgc tgccctccag cttgaccagg
     3961 gcccggaaga acttattgac ctcgacgtcg agcatctcga ccgacatctt gttgatgagg
     4021 tcgtcggccg aagggaccgc gccgcgctct ttgactacct ggtcgccgtt ggctccatgt
     4081 gacatttcaa tctccccagg gaactagctg cgctccgccg actattctct ttccgctaca
     4141 aacgtacggg aatgccgcgg gcggccatga tttctttggt ttgccggata gtgtactcgc
     4201 cgaaatggaa aatgctcgcc gccagggccg cgtcggcccg gccgatcagg atcgcgtcgg
     4261 ccaggtgctc cggccggccc gctccgccgc tggcgaccac gggaatggtc accgcccggc
     4321 tgacgctgct cgtaatctcc aaatcgtacc cgtccttggt gccatcgcaa tccatgctgg
     4381 tcaggacaat ctctcccgcc cccagttcct ccacccgcct ggcccaggaa accgcttcca
     4441 gccccgtcgg aacgcggccg ccgttgatga agacttcgaa ggagtgagtg gtgagtggtg
     4501 agtggtgggt ggtcgatcct gactcctggc ccactggtcc ctggctcctg gctactggct
     4561 cctggccgct ggcccctgac tcctgacccc tgaccctctt cgggtcaata ttcaccacaa
     4621 tacactggct gccgaaccgc cgggcggctt cggcgacgaa ctgcgggttc ttgcaggcgg
     4681 cggagttgat cgagacctta tcggccccgg cgttgagcag ggcgcggatg tcgtcgatcg
     4741 tgcggatccc gccccccacg gtcagcggca taaagacctg ctcggccgtg cggcggacca
     4801 cgtcgagcat gatgtcgcgc ccttcatggc tggccgtaat gtccaaaaac accagctcgt
     4861 cggccccttc ctgctcgtag cgggccgcca cctggactgg gtcgccggcg tcgcggaggt
     4921 tcaggaagtt cgtccctttc acgacgcggc cgcgatcgac atccaggcag ggaatcacgc
     4981 gcttggcaag cattacgaat ccggaccgca gcgcagaaaa ctcggaaagg ggcagcgagg
     5041 agcaacggcc agctggcgca ctgcactcta aatcgccgct gcgccgtggt caacgacgga
     5101 aggcgcggtc gcccgcgcga gcggacgagg agctgcagga ccaccatgcc cgattcgaat
     5161 cacgccatct tctcggtcaa gattctgcag cggcctgtca gagccgactg acggatggca
     5221 gcgatgcaac tacggccttg tccaattact ggacatattt tataatatgt acagtagagg
     5281 ttgcgcatcc gtgtttgcgt ggggcactgc gggtcgcggt ccaacggtaa gggataattc
     5341 ccgttctcgc ctctagtgct tatgtcagta atgtgagatc cgtcgcgcaa ctgctttccc
     5401 cacaagcgct tatgacaatt gaggcgattt caacttccgg cagcgagccg aattatcctt
     5461 acctcgaatt tcggtccgcg ccaggccaca gtctgacttg tcgttccagg gcccgtttcg
     5521 tacattccgc cgcccaaatt ggccatccag attcggcgac acatgaagca cctactgggc
     5581 atcctgatcg cggccgccgc aggcgcagca ggctgcgccg gaggagcctg gctggaaggt
     5641 ccgtcggacg ccgaccttcc gattcaaccg ccggcccgcg gctatccgac ggcgctgcgg
     5701 ccggtggcgg cgtcgatttc ggacgaggcg gccactccgc aaagtgcccc ggcggggccg
     5761 gcgcctagca cggctgtcct gccgctcgat cccaatcctc cgcccgctgc accgcctaag
     5821 cactcgcagc tgatcgatcc acggccgccg gctcccgcgc tggccgtggt gccaccccag
     5881 tatcagcggc caccagctcc gacagcgcca gcattgcagg ctctgccgcc cgcgactccc
     5941 attgcctcat cgctgccaag cgcggctgcg ccgccgcagg cgacagtcga gccggggctg
     6001 gcgccgattg tctcggccag ttcggtgcgt gccgcagctg ccagctctgc cgaccggcag
     6061 cccacgccac tgcagccgcc gctagcagcg gctgctgtcg cgccggcggc gacgagcggc
     6121 tccgttccca gcagcgagga gcggcgggcc gagattgagc gactccgcaa agagttcacc
     6181 gcggccttgg aagccgacat tcgcgagcgg caggcgagga atgccaagga cgaagagctg
     6241 ccgcgactgg agcagcaatt gcggctggcc tatttgctgg cagaccgcct ggacgacgcg
     6301 gtggcggccg tcgattcgct cgacccgccg cagcaggaag cctacaaaaa cctgatgttc
     6361 ggattgggcg tgtggctgtc gcccgacgag gcccaccgca cgccgctgcg gagtgcgaaa
     6421 gtgctgaagt cgctgcgcga ggcgacggcc gacctgtcta tggcgagcaa gctggagctc
     6481 cggaatctgg cgttctgcga gaaggtcgat tcctacggct ggtacacgga gtttccgaga
     6541 agcgagtttg cgcccaagca gcaggtaatc ctgtacgtcg aggtggagaa ctttgccgcc
     6601 gagcacaaga gcgccgccgg ctatgagacg gagcttcagg ggcggtacga aatcctcgat
     6661 gccagcggtc agatcgtcgc cgagcgggaa ttgccgctgg acaaggaagt ctgccgcaac
     6721 catcgccgcg actattatct gtggtaccgg atttacctgc ccgacgagat tgcgcccggc
     6781 aaatatcgcc tggagctgtc gatcgaagat cgcaaggccg gcgccaaata ccagggtcga
     6841 aagttcggcg aggggatgat cgagttcgcg attcggtagt aagatgcgct gcatcc
//
LOCUS       DASRJB010000675         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3301273, whole genome shotgun sequence.
ACCESSION   DASRJB010000675 DASRJB010000000
VERSION     DASRJB010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301273"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1312)
                     /locus_tag="VFB80_17945"
     CDS             complement(209..1312)
                     /locus_tag="VFB80_17945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN35720.1"
                     /translation="MWRASAAIVAILGLVTAAWAQETPPATTAAKSSAQKLKEDPNDV
                     QALNEYFEQAMSSAVKIMELQPEAARKQLQEISKSLGELKPSAPPAIKRVETAKVHVT
                     RYLKEIEIQQTPLADIEQKLTANPDDTEALSHYVFKMTQSIERAIKSSPDEAAKLLEA
                     FRAGLKKTSENAKEAATRTQAGTESRNTFRLERSIESARTQLAVIGKDAAPLDALAWV
                     NGSPLADNDLKGKVVLLDFWAVWCGPCIRSFPHLREWNEKYGEKGLVTIGLTRYYEFD
                     WNADAKTAVRVPGLSHEKEQEMLVKFAELHGLKYRFGLQSDESSLAKFYGVTFIPQVV
                     VIDQQGKVRLIRVGSGDEQAAAIAELLEKLLGP"
     gene            complement(1391..2536)
                     /locus_tag="VFB80_17950"
     CDS             complement(1391..2536)
                     /locus_tag="VFB80_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35721.1"
                     /translation="MSRLLTAILLSVPAAAWSQQVEPPRPAAAPAQPPKYNPNDPKSV
                     LDYINKEIDVVLHDRDALRSVLRLGELEALFAGLPQDAPELRDGIAQARIGILNMRDK
                     VYIHSITLEALLQKVRDDPDNFREVYCLGQKIFAEVYQPAFIVEPDECAKKVTAVREV
                     LAKVEQAAISDLTRKQLTRSLRTVDDLDKDIALSLETRKKQQALIGQDAAQFKVETWL
                     NGSELAADDLKGKVLLLDLFHAANFIPDDSDYQEWLGHLQRLAEWQKKYAERGLFVIG
                     LTGDNNVRWDEAKQAPVRVRAVKERVPQAEQLALLKRLIEKHELKHRVAIQQREEHFK
                     TIMYYPSLTQPHVIVVDRQGKIRLIRGGVSEKFTRDIDQALASLLGE"
     gene            2710..3639
                     /locus_tag="VFB80_17955"
     CDS             2710..3639
                     /locus_tag="VFB80_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN35722.1"
                     /translation="MPTYLEALTLRLAAGIAELPEEIRTRHAKYVLAAQRPDGGFAGR
                     EGGSDLYYTGFALRSLALLGELYGPPAERAAAFLASRLSGQESIVDFLSLVYGASLLQ
                     TAAGIDIFHDSALGWPDAVAAALARLRRPDGGYAKGAEGSASSTYHTFLVLLCLQLID
                     RQPEQPDQIVAFLHSQVCEEGGFREIRASKRAGTNPTAAAIGSLRILSSVDEETRRGT
                     IDFLAEMQTDEGGLRANTRIPIADLLSTFTGLLTLQDLGGAAEIDLDAARRYAQSLAL
                     DDGGFRGAEWDPAHDVEYTFYGLGTLGLLTEPA"
BASE COUNT          670 a         1298 c         1201 g          761 t
ORIGIN      
        1 tccgaacttc ttcgagaacc tgaccttcga gagcaactac gcttccggcg gattcggctc
       61 cggctacggc gactacggcg gtctctacga tcagatgtgg gattcctcgc tgcccgacgg
      121 cagctacctg ggaaccgatg gcgacacgcg ctactacacc ggcggctatt cgccgacaac
      181 agtgctgcgc cgacgctggc agcgtcggct acggacccag cagcttctcc agcagctcgg
      241 cgatcgcggc ggcttgctcg tcgccgctgc cgacccggat caggcggacc ttgccctgct
      301 gatcgatgac gacgacctgc gggatgaacg tgacgccgta gaacttcgcc aggctgctct
      361 cgtcggactg gaggccgaag cggtatttca ggccgtgcag ctcggcgaat ttgacgagca
      421 tctcctgctc tttctcgtgc gacagaccgg gaacgcgcac ggccgttttc gcatcagcgt
      481 tccaatcgaa ttcgtagtag cgcgtcaggc cgatcgtcac caggcccttc tcgccgtact
      541 tctcgttcca ctcgcggagg tgcggaaacg aacggatgca ggggccgcac cagacggccc
      601 agaagtccag cagcaccacc ttgcccttga ggtcattgtc ggccagcggc gagccgttga
      661 cccaggcgag ggcgtcgagc ggcgcggcgt ccttgccgat cactgccagc tgcgttcggg
      721 cgctttcgat ggagcgctcc agccggaacg tgttccggct ctccgtcccg gcctgcgtcc
      781 tcgtcgcggc ttccttggcg ttctcggagg tcttcttcag gccggcccga aacgcctcca
      841 gcagcttcgc cgcctcgtcc ggcgaacttt tgatggccct ctcgatgctc tgcgtcatct
      901 taaagacgta atgcgaaagg gcttcggtgt cgtcggggtt ggcggtcagc ttctgctcga
      961 tgtcggccag aggagtttgc tgaatttcga tctccttcag ataccgcgtg acatgcacct
     1021 tcgccgtctc cacccgtttg attgccggcg gcgcgctcgg cttcagctcg ccgagggatt
     1081 tcgaaatctc ctggagctgc ttccgggccg cttccggctg cagctccatg atcttgacgg
     1141 cactcgacat ggcctgctcg aagtactcgt tcagggcctg cacgtcgttg ggatcctcct
     1201 tgagtttttg ggcggacgac ttcgccgcgg ttgtcgccgg cggagtctcc tgggcccagg
     1261 ccgcggtcac caggccgaga atcgcgacaa tcgctgccga tgcccgccac atagttgaat
     1321 gctccgcgaa gacgaagagg tcctgcaacg gacacattag aagtgcggcc gtccaggctc
     1381 acaatagcag ctactcgccc agcagcgacg ccagcgcctg atcgatgtcg cgcgtgaatt
     1441 tctcgctgac gccgccgcga atcaggcgga tcttgccctg ccgatcgacg acgatcacgt
     1501 gcggctgcgt caatgacggg tagtacatga tcgtcttgaa atgctcctcg cgctgctgaa
     1561 tcgccacgcg gtgcttgagc tcgtgttttt cgatgaggcg cttgaggagg gcgagctgct
     1621 cggcctgcgg gacgcgctcc tttacagcgc gaacccgcac gggcgcttgc ttcgcctcgt
     1681 cccaccgcac gttgttgtcg cccgtcagcc cgatcacgaa caggccgcgc tcggcgtact
     1741 tcttctgcca ctcggcaagc cgctgcaaat ggccgagcca ctcttggtag tcggaatcat
     1801 cggggatgaa gttggcggcg tggaagaggt ccagcaacag cacctttcct ttcaggtcgt
     1861 ccgcggccag ctcgctgccg ttaagccagg tctcgacctt gaactgcgcc gcgtcctggc
     1921 cgatcagcgc ctgttgcttt ttgcgggttt ccagcgacag ggcgatgtcc ttgtcgagat
     1981 cgtctacggt ccttaaggag cgggtgagct gtttccttgt cagatcgctg atcgccgcct
     2041 gctccacctt ggctagcacc tcgcgcacgg cggtcacttt cttggcacat tcgtcgggct
     2101 ccacgatgaa tgcgggctga tagacctccg caaagatttt ctggcccagg caatacacct
     2161 cgcggaagtt gtccggatcg tcgcgcactt tttgcaagag cgcctcgaga gtaatggagt
     2221 ggatatagac cttgtcgcgc atgttgagga tgccgatcct cgcctgggcg atcccatcgc
     2281 ggagctcggg agcatcctgc ggcaagccgg caaacagcgc ttccagctcc cctagccgca
     2341 ggaccgaccg cagcgcgtct cgatcgtgca aaacgacgtc gatctccttg ttgatgtagt
     2401 cgaggaccga cttcgggtcg ttgggattgt atttcggcgg ctgagcgggt gcggccgccg
     2461 gcctgggcgg ctcgacctgc tgcgaccagg ctgcggctgg aactgagagc agaatcgccg
     2521 tcaataaccg cgacataaac tgcctcccga aagcgggtaa cggattaaac ctcgcccgcc
     2581 catccatcga ccgtcagccg aaccagaaaa accacgaaaa acgcgaaacg tcggaactgc
     2641 ctctttcgtg tctttcgtgt atttcgtggt tcccctgctc acccagccga ctacgatggt
     2701 cttggtccga tgcccaccta cctggaagcc ctgacgctcc gcctggctgc aggaattgcc
     2761 gagctgccgg aggaaatccg cacccgccat gcgaaatacg tccttgcggc ccagcgaccc
     2821 gatggcggct tcgccggccg cgaagggggc agcgacctct attacaccgg ctttgccctg
     2881 aggtcgctgg cgttgctcgg cgagctgtac ggcccgccgg ccgagcgggc ggccgcgttt
     2941 ctcgcctcgc ggctgtccgg gcaggaatcg atcgtcgatt tcctgtcgct ggtctatggg
     3001 gcgtcgctgc tccaaacagc cgcggggatc gacatcttcc acgactccgc tctcggctgg
     3061 cccgacgcag tggccgccgc gctggcccgg ctgcggcgtc ccgacggcgg ctatgccaaa
     3121 ggcgcggaag gctctgccag cagcacctac cacacttttc tcgtcctgct ctgcctgcaa
     3181 ttgatcgacc ggcagccgga gcagccggac cagattgtgg cgttcctcca ctcgcaagtt
     3241 tgcgaggaag gaggctttcg cgaaatccgc gccagcaagc gggccggcac caatccgacc
     3301 gcggcggcca tcggctcgct ccgcatcctg agcagcgtgg acgaggagac gcgccggggc
     3361 acgatcgact tcctggccga aatgcagacc gacgaagggg gcctgcgggc caacacgcga
     3421 attcccatcg ccgatctgct gagcacgttc accggcctgc tcacgctcca ggacctcggc
     3481 ggcgcggcag agattgacct ggatgccgcc cgccgctacg cccaatcgct cgccctggac
     3541 gacgggggct tccgcggcgc ggaatgggat cccgcgcacg acgtcgaata cacgttctac
     3601 ggccttggca cattggggtt gctgacggaa cccgcttagc cccggcgtct acgccggcgg
     3661 gatggagagg cgggcctcgt gggagagccg ggcgaaatgc agaatcgttc acgccctacc
     3721 cctctactga gggttcgaga cggaggtcgt ctgctataat ctgttcacta cagatcacga
     3781 cgttccgatc caagccgcgg ccttacaatc cagggccacg cagcgagtca caatcgcgtt
     3841 caaacccgcc acgacggctg gcaggtgcca tggatcaagg tggcgcacac agaaacagga
     3901 acagcaccgt gcgcccccgc atcacgatcc
//
LOCUS       DASRJB010000676         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2164200, whole genome shotgun sequence.
ACCESSION   DASRJB010000676 DASRJB010000000
VERSION     DASRJB010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164200"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFB80_17960"
     CDS             <1..280
                     /locus_tag="VFB80_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35723.1"
                     /translation="FGRTPTINARGGRDHYPKLCTLAFAGGGLAHGQIIGQSSKRNDA
                     PATAPYAPANLLSTLLHAQFDIGQMRLDPAVPRDLARFAETPPIAELF"
     gene            401..1744
                     /locus_tag="VFB80_17965"
     CDS             401..1744
                     /locus_tag="VFB80_17965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35724.1"
                     /translation="MKSESKPAAINPYDSAREAAEPLAGRPPSRGSLVVIFLTVFIDL
                     LGFGMVLPLLPVYAKSFGVDEHGWEIALLMSSFSAMTFLFSPLWGRLSDRIGRRPVLI
                     VGLVGSVGFYLLFGVATVWRSLPLLFVARIGAGIAGATIPAAQAYIADVTTLANRAKG
                     MAMIGAAFGLGFTFGPLLGAAALGWGAQASAAQVAASPGPGYAAAGLSALALLMAIFL
                     LPESLRPGAHPAGHSFFDLRAWRDAFTTPSVPALLATACASVVSFAAFETTLSLLLSS
                     EELQFRFSFRQVLLFFAFIGFMLSIAQGLLVRRLAPRVGEVPLCLAGGVVTIAGFALL
                     IAATRGGQLWMLLVAAAVEVTGFSLMTPSLQSLISRRSDPAKQGGILGVAQSTSSLAR
                     VLGPLIAVPLFHVSPPLPYVAAIGIMALSLVIFALFARRGVDHAAGAAVEYVPEL"
     gene            complement(1750..2385)
                     /locus_tag="VFB80_17970"
     CDS             complement(1750..2385)
                     /locus_tag="VFB80_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35725.1"
                     /translation="MSRVAQLLTLAAVASCAGCSLPVSVHPLTDQATSTIDERLIGHW
                     ETLKLGGEIDAGKDPQRFVIGRVQDKAKVLEMVGLELDGDTVKVNRTPAFATKLGDEC
                     YLSILNNPEEPKEKHVYLIQLYRVEDDRLKLFFMRPDVIGPAIERGDLAGEVVREPPD
                     ESAPPPEQVKPKYKSIKITASSKELAAYLTPRGTLPFHTQEFFQFRRVEPN"
     gene            2432..>2945
                     /locus_tag="VFB80_17975"
     CDS             2432..>2945
                     /locus_tag="VFB80_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN35726.1"
                     /translation="MSGWLIPASCRLIREIAMSQAALIDAYAAGPKLLREATRGMTRE
                     QALARPIAGKWSTLECVCHIADFEIVYADRLKRVIAENEPTMFGGDPDVFAARLAYER
                     RDLAEELNLIEACRGQVVRILRTLSDGDFQRIGKHSEAGPLTLQTLLERVTDHIPHHA
                     NFIQEKRAALK"
BASE COUNT          421 a          971 c          975 g          578 t
ORIGIN      
        1 tttcggccgc acgccgacga tcaacgcccg cggcggccgc gaccactatc ccaagctctg
       61 cacgctggcc tttgccggcg gcgggctggc gcacggacag atcatcggcc agtcgtcgaa
      121 acggaacgac gcgccggcca cggcgcctta tgccccggcc aatttgctct cgacgctctt
      181 gcacgctcag ttcgacatcg gccagatgcg gctcgatccc gccgtccccc gcgacctcgc
      241 ccgcttcgcc gaaactccgc cgatcgcgga gctgttctga tttcgtccgc tgcccggacg
      301 tctcacccga cagacctgtt gggccgtgta ctctgtacgg agtatgcagg acccagtacg
      361 aatacaaacc accgacacgt tcctcgctct agctcgccgc atgaagtccg aatcgaagcc
      421 cgccgcgatc aacccttacg attccgcgcg ggaagcggcg gagccgctcg ccggaaggcc
      481 gccgagccgc gggtcgctgg tggtgatctt cctgaccgtg ttcatcgacc tcttgggctt
      541 cgggatggtg ctgccgctgc tgcccgtgta tgccaagtcg ttcggcgtcg atgagcacgg
      601 ctgggagatc gccctcttga tgtcgagctt ctcggcgatg acgttcttgt tttcgccgct
      661 gtggggtcgg ctgtccgatc ggatcggccg gcggccggtg ctgatcgttg gattggtcgg
      721 ctccgtggga ttctatttgc tgttcggcgt ggccacggtc tggcgcagct tgccgctact
      781 gtttgtggcg cgaatcggcg cggggattgc cggcgcgacg attccggcgg cccaggccta
      841 catcgcggat gtgacgacgc tggccaaccg ggccaaaggc atggcgatga tcggcgcggc
      901 gtttggcctc ggctttacct tcggaccgct cctcggcgcg gcggcgctcg gctggggcgc
      961 tcaagcgagc gcggcgcaag tcgcggcgag tcccggcccg ggctatgccg ccgcgggatt
     1021 atcggccctt gcgctgctga tggccatttt tctgttgccc gagtcgctgc gccccggcgc
     1081 gcatcccgcc ggccattcgt tcttcgatct gcgggcgtgg cgcgacgcgt tcaccacgcc
     1141 gtcggtgccg gcgctgctgg cgacggcctg cgcgagcgtc gtgtcgtttg ccgcctttga
     1201 gacgacgctc tcgctcctgc tcagctcgga ggagttgcag ttccgcttca gcttccggca
     1261 ggtgctgctg ttctttgcct tcatcggttt catgctcagc atcgctcaag gcctgctcgt
     1321 ccggcggctg gcgccgcggg tcggcgaagt gccgctgtgc ctggcgggcg gagtcgtcac
     1381 gatcgccggg tttgccctgc tgatcgccgc cacgcgcggc ggtcagctgt ggatgctgct
     1441 cgtggcggca gcggtcgagg tcaccggctt ctcgctgatg acgccctcgc tgcagtcgct
     1501 gatcagccgg cggagcgacc cggccaagca gggcggcatc ttgggcgtcg cccaaagcac
     1561 cagctccctg gcccgcgtgc tgggtccgct gatcgccgta ccgctgttcc acgtctcgcc
     1621 gccgctgccc tacgtggcgg ccatcggcat catggctttg tcgctggtta tcttcgccct
     1681 cttcgcccgc cgcggcgtcg atcatgcggc gggagcggca gtggaatacg tgcccgaact
     1741 ttgaagccgc tagtttggct cgacgcggcg gaactgaaag aactcttgcg tatgaaacgg
     1801 cagtgtgccg cgcggcgtca ggtatgcggc cagttccttc gacgaggcgg tgatcttgat
     1861 gctcttgtat ttcggcttca cttgttccgg cggcggcgcg ctctcgtcgg gcggctcgcg
     1921 gacaacctcg cccgcgaggt cgccccgctc gatcgccgga ccgattacgt cgggccgcat
     1981 gaagaacagc ttgaggcgat cgtcctcgac gcggtacagc tggatcagat agacgtgctt
     2041 ctccttgggc tcctcggggt tgttgaggat gctcaggtag cactcgtcgc ccagctttgt
     2101 cgcgaaggcg ggcgtgcgat tgaccttcac cgtatcgccg tccagctcca gaccgaccat
     2161 ctccagcacc ttggctttgt cttgcacgcg gccgatgaca aaccgctgcg ggtctttccc
     2221 cgcatcgatt tcgccgccga gtttcagcgt ttcccagtgc ccgatcagcc gctcgtcgat
     2281 cgtcgaggtg gcctggtccg taagcgggtg gaccgagacg ggcaggctgc accccgcgca
     2341 agacgcaact gccgccaggg tcagaagttg ggctacgcgg ctcatgggac agcctccgcg
     2401 cggatggaac ggctcaccca gggaagatac catgagcggc tggcttattc ccgccagttg
     2461 ccgcctcatt cgggagattg ccatgtcgca ggccgctttg atcgatgcct atgccgccgg
     2521 gccaaagctg ctccgcgagg cgacgcgggg aatgacgcgc gagcaagccc tggcccggcc
     2581 gattgcgggc aagtggtcca cactggagtg cgtctgccac atcgccgact ttgaaatcgt
     2641 ttatgccgat cggctgaagc gggtgattgc ggaaaacgag ccgacgatgt tcggcggcga
     2701 cccggacgta ttcgccgcgc ggctggccta cgagcggcgc gacctggcgg aggaactgaa
     2761 cctgatcgag gcctgccggg ggcaagtcgt acgaattctg cggacgcttt cggacggcga
     2821 ctttcagcgg atcggaaagc acagcgaggc ggggccgctg acgctgcaga cgctgctgga
     2881 gcgcgtgact gaccacattc cgcaccacgc gaatttcatc caggaaaaac gcgcggcgct
     2941 aaaga
//
LOCUS       DASRJB010000677         6224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_198459, whole genome shotgun sequence.
ACCESSION   DASRJB010000677 DASRJB010000000
VERSION     DASRJB010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198459"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VFB80_17980"
     CDS             <1..1246
                     /locus_tag="VFB80_17980"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN35727.1"
                     /translation="DVEVSGFPSQHAGHLCLLRLKEDDYTYPQETEFDWQFSGQSGHF
                     KGTKTEQIGEWPTWDLPILDWGKKQGGVVGFSHSGWGLQLPDYGLNGERIPLQGRGPP
                     PGVQSRAADKLPDYAMPPFDGIGANEFVVDTVHGVCDFISSVDTPAVWELNVWYHTLN
                     CGMTTRISGETDFPCIYGDKVGLGRIYVKLDPAKQRQQLDFDSWVDGLRDGRSYVGDG
                     LSHLTEFSVNGFGVGERGDKGRASFLAAKSGQPLKVKVNVAAYLNAEPDTWNGQTIKS
                     LRLDQKPYWHLERSRVGATRKVPVELIVNGYPVEKREIEADGKLSELTFDYTPKTSSW
                     IAVRIFPSSHTNPVFVELDGKPIRASKRSAQWCLDAVETCWNQKVKQTRATEKETARA
                     AYDVAKQAYAKILEEAVDDRTN"
     gene            1271..2251
                     /locus_tag="VFB80_17985"
     CDS             1271..2251
                     /locus_tag="VFB80_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN35728.1"
                     /translation="MLVARFVSCTLCLLIAAAAFAKGPAYTDPAKTDSDFAFQGEYTG
                     TLKSNDGDRKFGLQVIALGGGKFHAVGYHGGLPGDGWDQGEKHEGDGELTDGVVTLKG
                     TAATATIKDGAVTIRNSDGDVLGELKKVERKSPTLGATPPAGAVVLFDGKSADAFSGG
                     KLTEDGLLQQGCTSKQEFGSCKLHIEFRLPYQPEDRGQARGNSGIYVGARYECQMLDS
                     FGLKGENNECGGLYSVKAPDVNMCLPPLAWQTYDIDYTAAVYEGDKLVKNPRVTIAHN
                     GVVIHKDVELPGERSTTAAPNKPGPEPGPIYLQDHGNPVRYRNIWVVETK"
     gene            2319..2759
                     /locus_tag="VFB80_17990"
     CDS             2319..2759
                     /locus_tag="VFB80_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35729.1"
                     /translation="MPAKDESDDFQLAPPDCPSREDRQRHLEPTQLSSPPPAPPPRHQ
                     YSVRDLMILMVGVAIGLAGGSWMPTDVFAATLGLLTLVGLLVVSWRPPETHLGKIIWA
                     MLVLAYVIAVFAAVFRPLSHPPRASGVPASQLPSTRRASSPLVF"
     gene            2826..4190
                     /locus_tag="VFB80_17995"
     CDS             2826..4190
                     /locus_tag="VFB80_17995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZN35730.1"
                     /translation="MSLTVVITILSMVALTALFAAYELALASVRTERLKALADRRVRG
                     AATALRMKNRMEASLAVAQLGMTMAGVIGAAAGGASVEEKLSPVLERWLHLDGPMADL
                     VALICFVVPLTAATVLIGELVPKVLAIKYAEWVCVTLSPLMWAVALVGYPVVLLVEWL
                     TTTIVALIESVLPSRPEDKHAGLHELRAQVNLLRASQVIGIQEERIILQASRLSSMKV
                     QDIMMPQDDIVMVVADATLSENLIVAHMDLHTRFPVTTKKGDPQAIIGYVTFKDMVLL
                     AKTHPGNPVIREIVRTITSLSENMSLSDALRRMTNEHHHLALVRDASGQVVGMIALED
                     IFEELLGDIQDEFDRLPRHISPAGHQLVVGGGVTLGQLRDLLKRPDIGGDLAPTTTLN
                     DWLNHGREEPLRGGDMVVVDRIWSQVRKVRRRRITEALLDPLGSPFEPVEKAARPAAP
                     SPVS"
     gene            4301..5176
                     /locus_tag="VFB80_18000"
     CDS             4301..5176
                     /locus_tag="VFB80_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35731.1"
                     /translation="MRILLAAALLGLLGSPGLARDIFVNNQTGDDVRDGSLAEAQGIL
                     GPCRTIAKALRIAQRGDQIVIANTGQPYRESITIQGPRHSGSERFPFTITGNGATLDG
                     TMSLAGATWEYAGHNTFRTRPPYLSFQQLFLDNQPAVRRQPSPGNAPELQPRQWCLFE
                     GWIYFRVDEGRLPHSYDLSSCGAQTGITLYEVHDVIVQDLVVRGFWLDGVNCHDNVRW
                     TSLARVATKENGRSGISIGGSSRVQLDTCTAAGNGAAQVRLEGFCRVQMLDNQLDAAT
                     APAVVKEGGTIVESK"
     gene            5293..>6224
                     /locus_tag="VFB80_18005"
     CDS             5293..>6224
                     /locus_tag="VFB80_18005"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN35732.1"
                     /translation="MFGIRYLKVPPTTYVLHYKGGQVKKQGPGLSFFYYSPTSEIVLV
                     PIESTDVPFVFNEVTADFQDATIQGELTYRVTDAKRAAEVLNFSVDDRGQHRSEDPQR
                     LGERLVHAGQIAARTFTQKRPLREVLIASDALVTEMTASLRNSPAVQMLGVEVLGLSI
                     LSIKPTPEMAKAMQAEAREEMLRAADEAIYARRNAAVDMERTIKENELKTEIAVEEKK
                     RQVRETQMAAEIAVEEQRKTLVDQRVENEKKEADARAHALQVTLEPLKSVDWKVLMAA
                     GGGNDPKLNIALAFRELAENAQKIGELNISPELLA"
BASE COUNT         1206 a         2000 c         2006 g         1012 t
ORIGIN      
        1 cgacgtggaa gtctccggct ttcccagcca gcacgccggc catctgtgtc tgctgcggct
       61 aaaggaagac gactacacct atccccagga aaccgagttc gactggcaat tcagcggcca
      121 gagcggccac ttcaaaggca cgaagaccga gcaaatcggc gagtggccga cgtgggacct
      181 gccgatcctc gactggggca agaagcaggg cggcgtggtc ggcttctcgc acagcggctg
      241 gggcctgcaa ctgcccgact acgggctcaa tggcgagcgg attccgctgc aaggccgagg
      301 gccgccgccg ggcgtgcaga gccgggcggc cgacaagctg cccgattacg cgatgccgcc
      361 cttcgacggc atcggggcca acgagttcgt cgtcgatacg gtccacggcg tctgcgattt
      421 catctcctcg gtcgatacgc cggccgtgtg ggaactgaac gtctggtatc acacgttgaa
      481 ttgcggcatg acgacgcgga tcagcggcga gacggatttc ccgtgcatct acggcgacaa
      541 ggtcggcttg ggacggatct acgtgaagct cgacccggcg aagcaacgac agcagctcga
      601 cttcgactcc tgggtcgatg gcctccgcga tggccgctct tatgtcggcg acgggctctc
      661 gcacttgacc gaattctcgg tgaacggttt cggcgtcggc gaaaggggag acaagggccg
      721 ggccagcttc ctcgctgcga agtcgggcca gccgctgaaa gtgaaggtca acgtcgcggc
      781 gtacctcaat gccgagccgg acacctggaa cggccagacc atcaagtcgc tgcggctgga
      841 ccagaagccc tattggcact tggagcggtc acgcgtcggc gccactcgca aggtgccggt
      901 cgagctgatc gtcaacggct acccagtcga gaagcgagag atcgaggccg acggcaagct
      961 gagcgagctg acgtttgatt acacgccgaa gacgtcgagc tggatcgccg tacgcatttt
     1021 cccgagctcg cacaccaatc ccgtattcgt cgagctggac ggcaagccga tccgcgccag
     1081 caaacgcagc gcccagtggt gcctggacgc cgtcgagacc tgctggaacc agaaggtgaa
     1141 gcagacacgg gccaccgaaa aggaaaccgc acgcgccgcc tacgacgtgg ccaaacaggc
     1201 gtacgccaag atcctcgaag aagccgttga cgatcgcacc aactaaatcc ctttcctgga
     1261 aaatactccc atgctcgttg cccgctttgt ttcctgcacg ctttgcctgc tgatcgccgc
     1321 ggccgccttt gccaaaggcc cggcctacac cgacccggcc aagaccgact ccgatttcgc
     1381 ctttcagggc gagtacaccg gcacgctcaa gtccaacgac ggcgaccgaa aattcggcct
     1441 gcaggtcatc gccctgggcg gcggcaagtt tcacgccgtg ggctatcacg gcggcctgcc
     1501 gggcgacggc tgggaccagg gcgaaaagca cgaaggcgac ggcgagctga cggatggcgt
     1561 cgtcacgctc aaaggaaccg cggcgacggc cacgatcaag gacggcgctg tgacgatccg
     1621 caactcggac ggcgatgtac tgggcgagct caagaaggtc gaacgaaaga gcccgacgct
     1681 cggcgcgacg ccgcccgcgg gcgccgtcgt gctcttcgac ggcaagtcgg ccgacgcgtt
     1741 cagcggcggc aagctgaccg aggacggcct gctgcagcag ggctgcactt ccaagcaaga
     1801 gttcggcagc tgcaagctgc acatcgagtt ccgcctgccc tatcagccgg aagaccgtgg
     1861 ccaggcccgc ggcaacagtg gcatctacgt cggcgcgcgg tacgagtgcc agatgctcga
     1921 ttcgttcggc ttgaagggcg aaaacaacga gtgcggcggg ctctattccg taaaggcccc
     1981 cgacgtgaac atgtgcctgc cgccgctggc ttggcagacg tacgacatcg actacacggc
     2041 cgccgtgtac gaaggagaca agctcgtcaa gaatccgcga gtcacgatcg cccacaacgg
     2101 cgtggtgatt cacaaagacg tcgaactgcc cggcgagcgc tcgacgactg ccgctcccaa
     2161 caagcccggc ccggagccgg ggccgatcta cttacaggat cacggcaacc ccgttcgcta
     2221 ccgcaacatc tgggtcgtcg agacgaagta gggcgcccca tcgtttaacc cggagccgga
     2281 ggcgacggcg gctacaggtt gaacgagcca ttgagtccat gcccgccaaa gacgaatctg
     2341 acgacttcca actcgcaccg cccgattgtc cttcgcgcga agaccggcaa cgccaccttg
     2401 agccgacgca gctatcgtct ccaccaccag cgccgccgcc gcgccatcaa tactcggtac
     2461 gcgacctgat gatcctgatg gttggcgtgg cgatcggact ggccggtggc tcgtggatgc
     2521 caaccgacgt cttcgcggcc acgcttggcc tgctcacgct cgtgggactg ctggtcgtga
     2581 gctggcgacc acccgagacc caccttggca agatcatctg ggctatgctc gtattggcct
     2641 atgtcatcgc ggtattcgcc gccgtctttc gaccgctgtc gcatccgccg cgggcttcag
     2701 gcgtgcctgc cagccaattg ccctcgacta ggcgagcctc cagcccgctt gtattctgag
     2761 gacgaggccc acccgcgaca gccctcgctg acgccgcgca agatgttact gcacctggag
     2821 ttgcgatgag tctgacggtc gtcattacga tcctgtccat ggtggctctg accgccctgt
     2881 ttgccgcgta tgagctggcg ctggcctcgg ttcgcaccga gcggctgaag gccctggcag
     2941 atcgccgcgt gcgcggcgcg gcgaccgcgc tgcgaatgaa gaaccggatg gaagccagtc
     3001 ttgccgtcgc ccagctcggc atgacgatgg ccggcgtgat tggcgccgcg gcgggcgggg
     3061 cgagcgtcga ggaaaagctt tcgcccgtgc tggagcgctg gttgcacctc gacgggccga
     3121 tggccgacct cgtcgcgcta atctgcttcg tcgtgccgct taccgcggcg accgttctga
     3181 tcggcgagct cgtccccaag gtgctggcga tcaagtatgc cgagtgggtc tgcgtcacgc
     3241 tcagcccgct gatgtgggcc gtcgcgctgg tcgggtatcc ggtggtgctg ttggtcgagt
     3301 ggctgacgac gacgatcgtg gccctgattg aaagcgtgct ccccagccgc ccggaagaca
     3361 aacacgcagg cctgcacgag ctgcgggcgc aggtcaacct gctgcgggcc agccaggtga
     3421 tcggcatcca ggaggagcgg atcattctgc aggccagccg cctgtcgtcg atgaaggtcc
     3481 aggacatcat gatgccgcag gacgacatcg tgatggtcgt ggccgatgcg acgctcagcg
     3541 agaatctgat cgtggcccac atggacctgc acacgcggtt cccggtaacg acgaagaaag
     3601 gggatccgca ggcgatcatc ggctacgtca cgtttaagga catggtgttg ttggccaaga
     3661 cgcatcccgg caacccggtg attcgcgaaa tcgtgcggac gatcaccagc ctgagcgaga
     3721 acatgtcgct ctccgacgcc ctgcggcgga tgaccaacga gcaccaccac ctggccctcg
     3781 tccgcgacgc cagcggccag gtcgtcggca tgatcgcgct ggaagacatt ttcgaagagc
     3841 tgctggggga cattcaggac gaattcgacc gcctgccgcg gcacatcagc ccggcggggc
     3901 atcagctcgt cgtgggcggc ggcgtgacgc tcggccagct gcgcgacctg ctcaagcggc
     3961 ccgacatcgg gggcgatttg gcccccacga cgacgctcaa cgattggctc aaccacggcc
     4021 gcgaagagcc gctcaggggc ggggacatgg tggtggtgga ccgcatctgg tcgcaagtcc
     4081 gcaaggtccg ccgccggcgc attacagagg ccctcctcga ccctctcggc agcccgtttg
     4141 agcccgtgga aaaggcggcc cggccggctg cgcccagccc ggtgagctaa gggaccggcc
     4201 actgtttgcc gcccctattt cgtgcataaa cggcatagtt gccgccggat caaaggcggc
     4261 gagctaagct tgcggtccat tccatgacag gaattgccaa atgcgaatac tgctcgcagc
     4321 cgctttgcta ggcctcctgg gcagcccggg gctggcccgc gacatttttg tcaacaatca
     4381 gaccggagac gatgtgcgcg acggatcgct ggccgaggcc caaggcatcc tcgggccctg
     4441 ccgaacgatc gccaaggcac tgcggattgc tcagcgggga gatcaaatcg tcatcgccaa
     4501 tacgggccag ccgtaccgcg agagcatcac gattcaaggg ccgcgtcata gcggcagcga
     4561 gcgttttcca ttcacgatta cgggcaatgg ggcgacgctg gacggcacca tgagcctggc
     4621 cggcgcgacc tgggaatatg ccggccacaa tacgttccgc actcgcccgc cttacctcag
     4681 ttttcagcag ctcttcctgg acaatcagcc ggcggtccgc cggcagccgt caccgggcaa
     4741 cgctccagag cttcagccgc gccagtggtg tctgttcgaa ggctggatct actttcgcgt
     4801 ggatgagggc cggctgccgc actcgtacga cctgtcgagc tgtggtgccc agaccggcat
     4861 cacgttgtac gaggtgcacg acgtgatcgt gcaggacctg gtggttcgcg gcttctggct
     4921 ggacggtgtc aactgtcacg acaatgtgcg gtggaccagc ctggcccgcg tcgcgaccaa
     4981 agagaacggc cgcagcggga tttccatcgg cggctcctcg cgggtgcagc tcgacacctg
     5041 cacggccgcc ggcaacggcg cggcccaggt ccgcctggaa ggtttctgcc gcgtgcagat
     5101 gcttgacaac cagctcgacg ccgccacggc cccagccgtc gtgaaagagg gcggcacgat
     5161 cgtcgagagc aaataatctc gaagattttt gcagccagtg ccaaggattg aactgcgggc
     5221 ccgaatcact tagtgtagat aggacactta ccaccgacgt cccgatcctc ctattcctga
     5281 ccccccacac ctatgttcgg catccgctac ctcaaagtcc cgcctacgac ctacgtcctg
     5341 cactacaagg gcggccaggt aaagaagcaa ggcccggggc tgtcgttctt ctactattcg
     5401 cccacatcgg agatcgtgct ggtgccgatc gagagcaccg acgtgccgtt cgtgtttaac
     5461 gaggtgacgg ccgattttca ggacgcgacg atccagggcg agttgaccta ccgcgtcacc
     5521 gacgccaaac gggccgcaga ggtgctgaac ttcagcgtcg atgaccgcgg ccagcaccgc
     5581 agcgaagacc cgcagcggct gggcgagcgg ctggtccacg ccggccagat cgccgctcgc
     5641 acgttcacgc aaaagcggcc gctccgcgag gtgctgatcg cctccgacgc gctggtgacc
     5701 gagatgaccg cgagcctgcg gaactcgccg gccgtgcaga tgctcggcgt cgaagtgttg
     5761 gggctgtcga ttctttcgat caagccgacg cccgagatgg ccaaggcgat gcaggccgag
     5821 gcccgcgagg agatgctgcg ggccgccgac gaggcgatct atgcccgccg caacgcggcc
     5881 gtcgatatgg agcggacgat caaggagaat gagctgaaga ccgagatcgc cgtcgaggag
     5941 aagaagcggc aggtccgaga aacgcagatg gccgcggaga tcgccgtcga agagcagcgg
     6001 aagacgctcg tcgatcagcg ggtggagaac gagaagaaag aagccgatgc cagggcccac
     6061 gcgctgcaag tcacgctgga gccgctcaag agcgtcgatt ggaaagtgct gatggcagcc
     6121 ggcggcggga acgatcccaa actgaatata gccctggcgt ttcgcgaact ggccgagaat
     6181 gcccagaaga tcggcgagct gaacatctcg ccggaactgc tggc
//
LOCUS       DASRJB010000678        16696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2164407, whole genome shotgun sequence.
ACCESSION   DASRJB010000678 DASRJB010000000
VERSION     DASRJB010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16696
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164407"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..381
                     /locus_tag="VFB80_18010"
     CDS             160..381
                     /locus_tag="VFB80_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35733.1"
                     /translation="MNSSIRALVGVDSEATAAESRAWETVAAADDRVILRESATGIET
                     LPMTRSAAESFLNAIQERAAARPERFVQS"
     gene            complement(378..839)
                     /locus_tag="VFB80_18015"
     CDS             complement(378..839)
                     /locus_tag="VFB80_18015"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HZN35734.1"
                     /translation="MAKSRAAWFDRLAKMAARVSGRPLTFFLALAIVLVWAVTGPLFD
                     FSDTWQLVINTGTTIVTFLMVFLIQSTQNRDTAALHIKLDELIRAVRGAHNDLLDLEE
                     LGEDELALIRDRYERLAALARQHLERGEADDDCPSVEESSARNSTPPSAER"
     gene            complement(947..1717)
                     /locus_tag="VFB80_18020"
     CDS             complement(947..1717)
                     /locus_tag="VFB80_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35735.1"
                     /translation="MKQRLICPLLAATALWAALGGGATPASAAIRVVIDAGSTTQVFY
                     SSGDAIGYFTTTLGNFDVAIGSALTNFTTQSQSGASLGQSLLISDAGGAALPTLTVTT
                     SVINAVGGVPDGLVTGGNIALVTGAGLARFTLPNAADLVVGSDIDGNSNLATGTIRNN
                     TTVNGSLVASLAIPIDGLGPDAQQTGIVSNNPAVGFTLVSQIVLTGASPGIMGSISAS
                     SNVSALVPEPGPLVLWGLGALGIVVAAARRRFTAPAAG"
     gene            2394..3236
                     /locus_tag="VFB80_18025"
     CDS             2394..3236
                     /locus_tag="VFB80_18025"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HZN35736.1"
                     /translation="MLLVCGIWSYWPSLVNLVGIWSRVVDYAHGFFVIPLALYFLWVR
                     RDRYPGCRESSPLLALAFLCLSLALRHVGDAFYFAFLDGWSLVPWAASVCALLGGRPL
                     LRWCWPSLLFLLFMVPLPFSLENNLSGPLQRVATVLSTQALQFLGQPGFAEGNVIVLG
                     DQRLEVAQACSGLRLFVGIVALTYAYVVVLRRPLWEKVALIAAAPAIAIVSNAARIVV
                     TGLLCRLLLPGPTARAWIHDGSGWVMILLAAGLFWLWLRYLKLLVKEEEVMDMMAVVK
                     QCRA"
     gene            3274..5490
                     /locus_tag="VFB80_18030"
     CDS             3274..5490
                     /locus_tag="VFB80_18030"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZN35737.1"
                     /translation="MNNRSHELALPASEPEVESAAGPLDAQIVLTSLRCWWKVAVPAS
                     LLLAAAAIVAVIYISRPKFTASAWLIIREKPEYLLNPQVMEDPRKFVQNQMELMRSPP
                     VIDPVASKPEVFATPELTGGDAAERLRRLLKIQSKGQSDFFVIEFTSVEPAKAALVVN
                     EVAKAYLQLQDRDLSQRMDATIGRLEKQRAAQQQSIEQLRKQVQEKTKSLTGVDPFAA
                     RSAAAQAAASDTLGPLSAQIVSAEIDHAMTSAQVGAEEELLAKQVFEVSAAEVEKQVQ
                     SLPEVVALRRRIGEAYGLLREHERVSVNLEKNTSYQRLLKQSNADKAQLEKRLGELRT
                     LMKTELEQQARLTRTDQVASLRKSLEAKEVTVRILRDRLQGEREKQQDYKGETVELEF
                     LRSDYDSAARVFEAINSRIVSMRLEQHAPDRVMMFKQATPPEFPTEALPYKRMSVIGL
                     AAFLAPFGLAVLVELIQRRVSNRRQLEVTSHMPVVAEVTAMPRITASRSMGRDFANRE
                     LHLFEESIDGLRTHLLLGPQADTMQTIAVTSAVSREGKTSVAIQLALSLARCGEAPTL
                     LIDGDLRCPEIHRIFEMDLSPGLAELLQQTCSQRAAIETEFGPTLHLLSAGKLSAVPH
                     KLLGSAEFPRLLADLKTRYRYIVLDTPPILSASEALMIARQADTSVVCARRDYSRLDQ
                     VAEAYSRLQSSGARVAGTVLNGIPPRAYAYRYGSYQYERALDREPALAPPTDVPGL"
     gene            5513..10171
                     /locus_tag="VFB80_18035"
     CDS             5513..10171
                     /locus_tag="VFB80_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35738.1"
                     /translation="MIRRTLSIPVFAVTAVGIVAAASGFHYVRGWQLSRLSKTFLDRA
                     REAQQREDWREAAEQFDHYLRLSPEQAAARTELALVFAKGAKSLDEKQRAVALHYRAL
                     ASALEDSTDELRIGLAELLLQTGRPLEAENAARDVLDRLPEEPRASRAFALARYMQWR
                     AGELGARSPDDLQLLAPLEEALERNPTDIALAEVTATLYREQPAIVEKHNPGLPPEKY
                     GPLADAVLDRAVELNVSNAEALLARHLYRAKYAQSDRERELAEQDLAKALVLAPQHPQ
                     ILLTASEARYQQARDALADPATRSAAAQPLVECKLLLERLIDGKLAPENSQPHLRLGD
                     VHVLDGELDLALDVWQDALGRFTRPAVRVAFHARIADHLLKAGRVEESKPSLTAIQEI
                     LDDLGGTIRRADHLALMQAQGLRQATYDLGTGRYAEAIAQLQRAIARQPQLQPNPQTS
                     HAAWDLMGRAFAGLQDWTAAATAFDRAANFQTEETGSRLAAAEAWLSAGRTELAIDRA
                     DQVIKQLEEKQRELDRKASQANQGVEQPRSEVKPVAGWSTAWLVLATAELQAQAVRPR
                     NERTWTQLEYALGTLEALPNRGGLAAPWRIDFLQADYLALRTDDEADDDRERRAAAEV
                     LRRAEVRYQDRPEFWFHACLAYERLAQPDDAERAWLQLNQFSGAQAEAAIAASRRAAA
                     HEDYSKAQNILESAAPELPPAAQQRLRQELIRLTKARQDLRQMQALFLATLRDRPHDV
                     GALCGLAELALRASDFPTLQKWEGQLAAAGPPGDVWAQYFRISRLYLSATGPRDPRLI
                     EALSEQARLSTLRPNWADGFSLRGAIEQRMEHLEAAAAAYEQAIQLGERRYVIFEQLI
                     TCLDQLNKGDEVEKYLARMQSYLPASQRLTELASARQLDENRPEQAIEIARRALERRP
                     GDLNARLWLARLLLVTDRLPEAQAEFEQATQQSPADARSWNGLFAYYLHVGDEQRARQ
                     TLESLEQHAQLDPASRNKLLGRAYAQLGDRSRAAELLARAVEQEPQSATSHLDLAQAL
                     LETDRGRAKENLQRALELDPKLARARWLLATILAASGSEADLAAAEQLLDEAGGTEAT
                     VEDRRVRAILLAQYSGDGGLNRAILMLEQIIAGDTESTTDRLLLAQFYERRAAASRDA
                     AEAAGNVQKARDQLLNVASRTRAQAADVGVLISFLLRQDRKDEAARWLDRLEDRLAGQ
                     ARPDPKSIALLIDLRLAQGSVKRCEPWLAKLEATDRDPFRPLIARVKYFAALPNIEAI
                     EPAVEAQALRLIQATRSLKEQSRIARQIGDLYLLVNQLAGAEKWYRTVVKSDPDQFPL
                     LATALVRQNRGLEAIRLCETAAENDTTSRPAIVLTSLLLESGAKREYMRAAEPILAAA
                     LKNFPDDPNLVYGVGMLRVLEDRYPEAIVLLRKVVRLNPRHVPALNNLAMLLAESEET
                     RAEGLAFVERAIVHRGHLPTLLDTKGTILLWSGKTQEAVTLLEAAARGSTTDPRHSFH
                     LASAYHELGSADNAQEQLAAALERHLEKQILTPTDREALNKLRTALTRGAVINSPRPR
                     "
     gene            10185..10886
                     /locus_tag="VFB80_18040"
     CDS             10185..10886
                     /locus_tag="VFB80_18040"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HZN35739.1"
                     /translation="MSRYIVGAFLLLGTLGSGWLHGRLVNRWGQASPLATAAARLQGG
                     LPDHLGHWQLVKTHELEEGVADILQCAAYLHGVYTNDQTGDTIVVALVAGPSGPISVH
                     TPEICYSAQNYEIAGDRELMKVRDLHGNTHQVWQVFANSRDSARPNLRVIYGWSRGAN
                     WEAVAGPRFAYAGLPVLYKLQLAGPAREHRSSQPSADACQDFLSEFLAQIQPRLISAP
                     GGDSFIHPSAPKGVL"
     gene            10886..11701
                     /locus_tag="VFB80_18045"
     CDS             10886..11701
                     /locus_tag="VFB80_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZN35740.1"
                     /translation="MSLTQPADVVWSENVCPLGQKCKSDAVRAIIERVMGLLLLVLVS
                     PLIVALVVVIRLTSPGPGIYCQRRVGLRGRIFTMYKLRSMRSDAENRSGPVWASVVRD
                     ARVTPLGYWLRRLHLDELPQLYNVARGEMALVGPRPERPEFVQILAEQIPEYLDRLEV
                     KPGITGLAQINLPPDSDLQSVRRKLVLDQEYLRCAGPLFDARIVLCTALRVFGLRGGR
                     AVSLMGLSRTVELPPVCDASTQSAPPVAASAPAQPEHDRRPLSHPAPLIVARS"
     gene            11728..13035
                     /locus_tag="VFB80_18050"
     CDS             11728..13035
                     /locus_tag="VFB80_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN35741.1"
                     /translation="MDFVVFVLVNAALFLRPQDLIPDLAEVPIYNVLIVFNLVVAAPH
                     LVRELRSEPRRSPATICVVAILVAAVLSLLIKFDWRAAWQAGLQFAKVGAYFLLITAV
                     LRSPRRYTTFLALVVILTTVLAAIAVAHFHGQLNVAAITHAREVTYDAATGDQSSALR
                     LSAFGVFADPNDLSMVVVLSILIGLGGICYPKLGMLRVPLIGVVLFLGYTLALTQSRG
                     GLLALVAGLGAFVLCRYGVSRMSLALVALVPLLFVVFGGRQADISGGIASGTGSSRTD
                     LWYAGLQMIKWQPLWGVGHGRFVEHEGLVAHSSYLQALAEWGVLGGAMFVGLFYVVLY
                     SIWRLRRFRPMIGSGVLRSFHPFVLGALAAYATSMLTLTRCEVVPTYLVAGLGVSYER
                     LARRNVPLRPLEFTPQLFSAMLGVSLAYLAVLYVFIRFIYRMF"
     gene            13087..13896
                     /locus_tag="VFB80_18055"
     CDS             13087..13896
                     /locus_tag="VFB80_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN35742.1"
                     /translation="MIEPSRGWQLINLRELWQYRELFGFLIWRDVKVRYKQTLLGAAW
                     AILQPALMMIVFTVFFARLARVPTGNLPYPLFVYAGLLPWLLFSTSVSQAAQSVLSSE
                     RLITKVYFPRLAIPLASVGAAIVDFVIALVLLLGLMLWYGVTPTAGLLVVPLVMGCLL
                     LAATGVGTAIAALNVAYRDFRYLVPFLLQMWMFATPTIYMDPAANGAGRLTSLLALNP
                     LAPLVSTFRAATVGEPISWLGLLLSAAVAGVMFLAGCAIFRRFEDEFSDII"
     gene            13909..15051
                     /locus_tag="VFB80_18060"
     CDS             13909..15051
                     /locus_tag="VFB80_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35743.1"
                     /translation="MYHAAAYIHDEISLYNASKAMAGLLELAELGEITLRLTDRGFAA
                     PHPAVFQLTVAEDDGEPQRAAIDLLDRSDVVDFATLASCDVYFKRSYFQADLAKLSQQ
                     SWLRPQTAKIRPWGLNFPCLVPALKSRRWKLLARCFWKRSLRALVRGPQAARAVAQEM
                     RQFACLPAAAQFEADPGEPLEPIVVFQTRLWEPHEAAPDDAAAVNRMRVEVVRALRQA
                     LSTAFVGGLVSTPLARREHPGEVVPPQQSRMVNYAALSRRALIGVYTRGLHHSTAFKL
                     PEYLAGSKAVVAEGLRNQTPTPLERGLHWAEFRDADECVLECRRLLADHERCAAMRQA
                     SAEYYRLHVAPQAHLRWTLAEACAARPASAAAPRLVTQEPELARSL"
     gene            15048..16331
                     /locus_tag="VFB80_18065"
     CDS             15048..16331
                     /locus_tag="VFB80_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN35744.1"
                     /translation="MTAAVRIENLSKRYFVDHENRSHVSYRTLRESLAHLAATPLRRL
                     QGQRRATREEFWALREVSLEVEAGEVVGIIGRNGAGKSTLLKILSRITKPTRGRVELT
                     GRVGSLLEVGTGFHPELTGRENVYLNGSILGMQKREIDARFDQIVDFAEIEQFLDTPV
                     KRYSSGMYVRLAFAIAAHLHPEVLIIDEVLAVGDTAFQRKCLGSMESLGKSGRTVLFV
                     SHDLNAVAKLTTRSFLLERGRLTASGNTPDVIHSYLRCSEGYSSEWCRQGPPHAERPT
                     ILRIVALNPDGQRCSLFDADQPFEIEFQCHFPRAVEAQLAFRLNADFQTETIFTSSLA
                     DGEDRATRIGEGLVTARCTVPAHLLIPGRYHVLAAINNFKGPQFDMVERALAFEVTEI
                     GSLTKNDGRLGKVAPLLEWTCTQGAAPPALATHPC"
     gene            16325..>16696
                     /locus_tag="VFB80_18070"
     CDS             16325..>16696
                     /locus_tag="VFB80_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35745.1"
                     /translation="MLTAPLTSIYRTLLPPQVRAPLWHLRSAGGALLSDRPSYRQWLL
                     DFYRSIGRFRPVVRTAIGTRLAVDLRDEGIGWPIFVHQEYEPGETRFIARTLRAGDVF
                     VDVGANVGYFTTLASGRVGPRG"
BASE COUNT         2902 a         5335 c         5360 g         3099 t
ORIGIN      
        1 cgcgggacgg atgggacgtg gtttcgctgg acgggcgagc ggtgggaacg gtcgcgctaa
       61 agccgaaaaa cacctgccga cagtgtgtga cgcagggcga caacctgtcg cgatgctccg
      121 cggcccggcc ggcacgcgat ctgctcatgg ccgcccgcca tgaatagcag catccgagcg
      181 ttggttggcg tcgattcaga ggcgactgcg gccgagtcga gggcatggga aaccgtggct
      241 gcggcggacg accgggtgat cttgcgggaa tccgcaacgg gcatcgagac gctgccgatg
      301 acccgcagcg cagcggagtc attcctgaac gcgattcagg agcgagccgc cgcccgccca
      361 gagcgtttcg tccaaagtta gcgctcggcg ctcggcggcg tagagttccg ggcgctcgat
      421 tcttccacgc tggggcaatc gtcgtctgcc tcgccgcgtt ccaggtgttg acgggccaga
      481 gcggccagcc gttcgtaccg atcgcggatg agcgccagct cgtcctcgcc cagttcttcc
      541 aggtcaagca gatcgttgtg ggcgccgcga acggcgcgaa tcagttcgtc gagcttgatg
      601 tggagggcgg ccgtgtcgcg gttctgcgtg ctttggatca agaacaccat cagaaaggtg
      661 acaatcgtcg tgcccgtgtt gatgacgagc tgccacgtat cgctgaagtc aaacagcggc
      721 ccggtcaccg cccaaaccag cacgatggcc agcgccagga agaacgtgag cggccgcccc
      781 gacacccgcg cggccatttt tgccagtcga tcaaaccagg cggcacgaga cttggccatg
      841 ttgttcgaag ccgcggcaga aacaaagagc ggacggacag ccgcgagcga ccgcccgtcc
      901 gcaagagttc taagcgctaa aaactcttcc aaccagccgc ggctgatcac ccggctgccg
      961 gggcagtgaa gcggcggcgc gcggccgcca cgacaatgcc cagtgcaccc aagccccaga
     1021 ggaccagcgg acccggctcg ggaaccagcg cactcacgtt ggatgaggcc gagatcgacc
     1081 ccatgattcc gggtgaggcg ccggtcagga caatctgcga gacgagcgta aagcccaccg
     1141 caggattgtt gctgacgatg ccggtctgct gagcatcggg acccaaacca tcgatgggga
     1201 tcgcgaggct ggcgacgagg ctgccattca ccgtcgtatt gttgcgaatg gttcctgttg
     1261 ccagattcga attcccgtcg atgtccgacc cgactacgag atctgcggcg ttcggcagcg
     1321 tgaagcgagc cagcccggct cccgtcacga gcgcgatgtt gccgccggtg accaatccat
     1381 cgggaacgcc gccgacggcg ttgatcacgg aggtcgtgac cgtcagcgtc ggcagagccg
     1441 cccccccggc gtcgctgatc agcagcgatt ggccgagcga tgcaccggac tgcgactgtg
     1501 tcgtgaagtt ggtcagagcg cttcctatcg caacgtcgaa gttgcccagg gttgtggtga
     1561 aataccctat ggcatcaccc gaggagtaga acacttgcgt ggtcgaaccg gcgtcgatca
     1621 cgacccggat tgcggcgctg gcaggcgtcg ctcctcctcc aagcgccgcc cacagggcgg
     1681 tcgccgccag caacggacag ataagtcttt gcttcatcca ttttcctcca caggttggag
     1741 acgccgcgca ccccgggcaa cgctgcccgg acgcgacacc ttgacacaca attccattgc
     1801 ccggcggcaa aaacccagac gcttggaaca aatctcgtgc cgcagcgcag aggattgccg
     1861 caggtgcaag gctgggcgat agaggcggcg attcgccgca caccgcgaca caccgcgaca
     1921 cgcgcgggtc aacttgtccg gtgctggcgt cgcgccaagc ccggcatcgt ttgcgcggtg
     1981 cgaagacgtc gcgctttgag cgtccatttc gcgcgtagtc attggcctcg ccggggcgct
     2041 ggggccggca tgttctttgc ggtcgctctg gcccgcctcc gaggcgctca gggctttggg
     2101 tgcaaacacg gatcagagcg gcggggaaca caaggacagt ttcgtttggt gtcatttctt
     2161 ccgacttcgc tggccggcga agtcggattg cgattgacgc cgcaactcga tgcctggtga
     2221 ggcccgccac catgacgacg ccgactgcct cgcgccacgc caagcggaac tcaccgctgc
     2281 cgcgtgcctc ccagcgccgg caacacgcgg tcaaggaacg cttggccccc gttcccgctc
     2341 caaacaaaga ctcgccggat aaagccgtca gagggcgcgt attctttgcg accgtgctgc
     2401 tggtttgcgg aatctggtcg tattggccct cgttagtgaa tctcgtcggc atctggagcc
     2461 gggtagtcga ctacgcgcac gggtttttcg tcattccgct ggccctgtat tttctgtggg
     2521 tgcgccgcga ccgctacccc ggctgccgcg aatcgagccc cctcctggcg ctcgccttct
     2581 tgtgcctgag cctggcgctg cggcacgtgg gcgacgcctt ctacttcgcc ttcctcgacg
     2641 gctggtcgct ggtcccgtgg gctgcatccg tgtgtgcctt gctcggcggc cggccgctgc
     2701 tgcgatggtg ctggccatcg ttgttgtttt tgctgttcat ggtgccgctg ccattctctt
     2761 tggagaacaa cttgagcggc ccgctgcaac gcgtcgccac ggtgctcagc acgcaggccc
     2821 tacagtttct ggggcagccg ggctttgccg aaggtaacgt catcgtgctc ggcgaccaga
     2881 gactggaagt ggcgcaggcc tgttcggggc tgcgactgtt cgtgggaatt gtcgccctga
     2941 cgtatgccta tgtcgtcgta ctgcggcggc ccctgtggga aaaggtggcc ctgattgccg
     3001 cagcaccggc gatcgccatc gtctcgaatg cggcccggat cgtcgtaacg ggactgctct
     3061 gccggttgct gctgccgggg cctactgctc gagcctggat tcacgacggt tcaggctggg
     3121 tgatgatcct gttggcagcg ggcctgtttt ggctgtggtt gcggtatttg aagctgctcg
     3181 tcaaggagga agaggtgatg gacatgatgg ccgtcgtcaa gcaatgccgt gcgtgagtca
     3241 cccgttcgcc ggttcccacc tgccagtccc gccatgaaca accgctccca cgaattagcg
     3301 ctgcctgcca gcgagcccga agtggaatcg gccgccggcc cgctcgacgc tcagatcgtg
     3361 ctcacgtcgt tgcgctgctg gtggaaagtg gccgtgccgg cttcgctctt gctggcggct
     3421 gcagccatcg tggcggtcat ctatatctcg cggccgaagt tcaccgcctc cgcctggctg
     3481 atcatccgcg agaagcccga atatctgctc aatccgcagg ttatggagga tccgcgcaaa
     3541 ttcgtgcaga accagatgga gctgatgcgc agcccgccgg tgatcgaccc cgtggccagc
     3601 aagcccgaag tgttcgcgac gcccgagctc accggcggcg atgcggccga acgcctgcgg
     3661 cggctgctca agatccagtc caaggggcag tccgactttt tcgtcatcga gttcaccagc
     3721 gtggagcccg ccaaggccgc gctggtcgtc aacgaggtgg ccaaggccta cctgcaactg
     3781 caggaccgcg acctgtcgca gcggatggat gccaccatcg gccggctcga aaaacagcgc
     3841 gccgcccagc agcagtcgat cgagcagttg cggaagcagg tccaggaaaa aacgaaatcg
     3901 ttgacgggcg ttgatccttt tgccgcgcgc agcgccgcgg cgcaggctgc cgcgtccgac
     3961 acgctcgggc cgctgtcggc gcagatcgtg tcggcggaaa tcgaccacgc gatgacctcc
     4021 gcccaggtcg gggccgaaga agagctgctg gccaagcagg tctttgaggt ttcagcggcc
     4081 gaggtcgaga aacaggtaca gtcactgccc gaagtcgtcg cgctgcgcag gcggatcggt
     4141 gaggcgtacg gcctgctccg cgagcacgag cgcgtctcgg tcaatctgga aaagaacacc
     4201 agctatcagc ggctgctgaa acaatccaac gccgacaagg cccagctcga aaagcgcttg
     4261 ggcgagctgc gaacgcttat gaaaaccgag ctggagcaac aggcccgcct gacccgcacc
     4321 gaccaggtcg ccagcctccg caaatcgctg gaggccaaag aggtcacggt caggatcctg
     4381 cgcgatcgac tccaaggtga gcgagaaaag cagcaggact acaagggcga gaccgtggag
     4441 ttggagtttc tgcggtctga ctacgacagt gccgcccgcg tcttcgaggc gatcaacagc
     4501 cggatcgtct cgatgcggct tgagcagcac gcccccgacc gcgtgatgat gttcaagcag
     4561 gcgacgccgc ccgagtttcc gaccgaggct ctgccctaca aacggatgag cgtgatcggg
     4621 ctggccgcgt tcctggcccc gttcggcctg gcggtcctcg tcgaattgat tcagcgccgc
     4681 gtcagcaatc gccggcagct cgaagtcacc agccacatgc cggtcgtggc ggaagtcacc
     4741 gcgatgccgc ggatcacagc gtcgcggtcc atgggccgcg actttgcaaa tcgcgagctg
     4801 cacctgttcg aggagagtat cgacggcctc cgcacgcatc tgctgctcgg cccgcaggcc
     4861 gacaccatgc agacgatcgc cgtgaccagc gccgtcagcc gcgaaggcaa aacgagcgtg
     4921 gcgatccagc tggccttgag cctggcccgc tgcggcgagg cgccgacgct cctgatcgac
     4981 ggcgacctgc gctgccccga gattcaccgc atcttcgaga tggatctctc gcccggactg
     5041 gccgagctgc tgcagcaaac atgttcgcag cgggcggcca ttgaaaccga gtttggcccg
     5101 accctgcacc tgctctcggc cggcaagctg tccgccgtcc cgcacaagct gctgggcagc
     5161 gccgagttcc cgcggctgct ggctgatctg aaaacgcgct accgctacat cgtgctcgat
     5221 acgccgccga tcctctcggc cagcgaggcc ctgatgatcg cgcggcaggc cgatacatcc
     5281 gtcgtttgcg cccgccgcga ttacagccga ctcgaccagg tcgccgaggc ctattcccgg
     5341 ctgcaatcgt ccggggcgcg agtggccggc accgtgctca acggcattcc cccgcgggcc
     5401 tatgcctatc ggtacggcag ctaccaatac gaacgtgcct tggacaggga gccggcgctc
     5461 gcgccgccta ccgacgttcc gggcctgtaa gcgcaagatt cgccaatccc agatgattcg
     5521 ccgtaccctc agcattccgg tcttcgccgt taccgcggtc ggcattgtcg ccgcggccag
     5581 cggctttcat tacgtccgcg gctggcaact ctcgaggctg tcgaagacgt ttctggaccg
     5641 cgcccgcgaa gctcagcagc gagaagactg gcgggaagcc gccgagcagt tcgaccacta
     5701 tctgcggctg tcgccggagc aagcggccgc ccgcaccgaa ctggcgcttg tgtttgccaa
     5761 aggcgcgaaa tccctggacg agaaacagcg cgccgtcgcg ttgcactacc gggcgctggc
     5821 ctcggcgcta gaggactcga cggatgagct tcgaatcggc ctggccgagc tgctgctgca
     5881 gaccggccgg ccgcttgaag ccgaaaacgc ggcccgcgac gtgctggaca ggcttccgga
     5941 agagccgcgg gccagccgcg ccttcgcact cgcccgctat atgcagtggc gggccggcga
     6001 attgggcgcc aggtcccccg acgacctgca gctgctcgcg cctctcgagg aggcgctgga
     6061 gcgaaacccc accgacattg ctctggccga agtgacggca acgctttatc gcgagcagcc
     6121 cgcgatcgtc gagaagcaca accccggcct gccaccagag aagtacggcc cgctggcgga
     6181 tgctgtgctc gaccgggccg tggagctcaa tgtgtcgaat gccgaggccc tgctggcccg
     6241 gcacttgtat cgcgctaagt atgcccagag cgacagggag cgcgaactcg ccgagcagga
     6301 tctcgccaaa gcgctggtcc tggcgcccca gcatccgcaa atcttgctga ccgccagcga
     6361 ggcccgttat cagcaagccc gcgacgcact cgcggaccca gcgacgcggt cggccgccgc
     6421 tcagccgctg gtcgaatgca agctgctgct tgagcggctt atcgacggaa agctcgcgcc
     6481 ggagaattcg cagccccatt tgcggctggg cgatgtgcac gtgctggacg gcgagctcga
     6541 cttggcctta gacgtttggc aagacgccct cgggcggttt accaggccag cagtgcgtgt
     6601 cgcctttcat gcccgcatcg ctgatcacct tttgaaggcg ggccgggtgg aagaatccaa
     6661 gccctcgctg accgcgattc aggaaatcct tgacgatctg ggcggcacga tccgccgcgc
     6721 ggaccacctg gccttgatgc aggcccaggg tctgcggcag gccacgtacg acctcggaac
     6781 tggccgctac gcggaggcga tcgcccagtt gcagcgggcc atcgctcggc agccgcagct
     6841 tcagccgaat ccccagacgt cgcatgcggc ctgggacttg atgggccgcg ccttcgcggg
     6901 ccttcaagac tggaccgctg ccgccaccgc gttcgaccgg gcagccaatt ttcaaaccga
     6961 agagaccggg tcgcgtctgg cggcggccga ggcctggctc tccgcggggc gaacggagtt
     7021 ggctatcgac cgggccgatc aagtcatcaa gcaactggag gagaagcaga gggaactcga
     7081 tcgaaaggcc agccaggcca atcaaggggt cgaacaacct cgcagcgaag ttaagccagt
     7141 cgccggctgg tctacggcct ggctcgtgct ggcgacggcc gaattgcagg cgcaggcagt
     7201 ccggccgcgg aacgagcgaa cctggacgca gttggagtac gccctgggaa cgctggaggc
     7261 gctgcccaat cgcggcgggc tcgcggctcc ctggcggatc gattttctgc aggccgatta
     7321 cctggcgctg cggacggatg acgaagccga cgacgatcgc gagcggcgag cggcggccga
     7381 agtgctgcgg cgggcggagg tgaggtacca ggatcgcccc gagttctggt tccacgcctg
     7441 cctggcctac gagcgactcg cgcagccgga cgatgctgag cgggcctggc tgcagctgaa
     7501 ccagttttca ggcgcgcagg ccgaggccgc gattgcggcg tcgcggcggg ccgcggccca
     7561 cgaggactac tccaaggctc aaaacattct ggagtcggcg gcgcctgaat tgccgcctgc
     7621 cgctcaacaa cggctccgcc aggaactgat ccgcctgacg aaggccaggc aagacctgcg
     7681 gcaaatgcaa gccctgttcc tggcgacgct gcgcgaccgg ccgcacgacg tcggggcact
     7741 ctgcggtctg gccgagttgg cgctgcgcgc cagcgacttc cccacgctgc agaagtggga
     7801 aggccagctc gcggcagccg gcccaccggg tgacgtgtgg gcccagtact tccgcatttc
     7861 gcggctctac ttatccgcca ccggaccgcg cgacccgcgg ttgattgaag ccctgtccga
     7921 acaggcccgg ctgtcgacgc tccggcccaa ctgggcggat gggttctcgc tacgaggagc
     7981 gatcgagcag cggatggagc atttggaggc cgccgccgcc gcctacgagc aggccattca
     8041 gcttggcgag cgccgctatg tcatcttcga gcaactcatc acctgcctgg accaattgaa
     8101 taaaggggat gaagtggaga agtacctggc gcggatgcag tcgtatctgc ccgcttccca
     8161 gcggctcacg gagctggcct ccgcccgcca gctcgacgaa aatcgccccg agcaagccat
     8221 cgagatcgcc cgccgcgccc tggagcggcg gcccggcgat ctcaacgccc ggctatggct
     8281 ggcccgcctg ctgctggtta ccgaccggct gccggaagcc caggccgaat tcgagcaagc
     8341 cacgcagcag tcgccagccg acgcccgcag ttggaacggc ttgtttgctt attacctgca
     8401 cgtcggcgac gagcagcgtg cccgacaaac gctcgagtcg cttgagcagc acgcgcagct
     8461 cgacccggcg tcacgcaaca agctcttggg tcgggcatat gcacaactgg gtgatcgctc
     8521 gcgggccgca gagctgttgg cgcgggccgt cgagcaggaa ccgcaatcgg cgacgagcca
     8581 tttggacctc gcccaggctt tgctcgagac cgaccgcggc cgggccaagg agaatttgca
     8641 gagggccctg gagctggatc ccaagctggc gcgggcccgc tggttgttgg cgacgatctt
     8701 ggcggcgtcc ggcagcgagg cggacttggc ggccgccgag cagctcttgg acgaggccgg
     8761 cggcacggaa gcgacagtcg aggaccggcg agttcgggcg atcctgctgg cgcaatacag
     8821 cggcgacggg ggactaaatc gggccatctt gatgctcgag cagatcatcg cgggcgacac
     8881 ggaaagcacc accgaccgct tgctcctggc gcagttctac gagcgacggg cagccgcctc
     8941 cagggacgcg gccgaggcgg cagggaatgt ccaaaaagcc cgcgatcaat tgctgaatgt
     9001 ggccagccgt acgcgggctc aggcggcgga tgtgggagtg ctcatcagct ttctgctgcg
     9061 gcaggatcgc aaagacgaag cggcacggtg gctcgaccgg ctggaagatc gcctcgcagg
     9121 acaggccagg cccgatccca agtccatcgc cctgctaatc gatttgcggc tcgcgcaggg
     9181 ctcggttaag cgttgcgagc cttggctggc gaagctcgag gcgaccgaca gagatccatt
     9241 tcgtcccttg atagctcgcg tgaaatactt tgcggccttg ccgaatatcg aagccatcga
     9301 gccggcagtg gaggcccagg cgttgcgtct cattcaggcc acgcggtcgc tcaaggaaca
     9361 aagccgcatc gcgcggcaga ttggcgattt gtatttgctc gtcaatcagc tggccggcgc
     9421 ggagaagtgg tatcgcacgg tcgtcaaatc cgaccccgat cagtttccgc tgctggcgac
     9481 cgcgctcgtt cggcagaacc gcggcctgga agcgattcgc ctgtgcgaga cggcggccga
     9541 gaacgatacc accagccggc ccgccatcgt cctgacttcg ctgctgctcg agtcgggcgc
     9601 gaaacgggaa tacatgcggg cggccgagcc gatcctggcc gcggcgctga aaaacttccc
     9661 agacgacccg aatttggttt acggcgtcgg catgctacgc gtgctggaag accgctatcc
     9721 cgaggcgatc gtactgcttc gcaaagtcgt taggctcaac ccgcgtcacg tgccggcgct
     9781 caacaacctg gcaatgctct tggccgagtc ggaggaaacg cgggcggagg gattggcgtt
     9841 cgtcgaacga gcgatcgtgc atcgcgggca tctgccgacg ttgctcgaca ccaagggcac
     9901 gatcctgctg tggtcaggca agacacaaga ggccgtcacg ttgctggaag ccgccgcccg
     9961 tggatcgacg acggatccaa ggcacagctt tcatttggcg tcggcctatc acgagctggg
    10021 atcggccgac aacgctcagg agcaactggc agccgccctg gagcgccacc tcgaaaagca
    10081 aatcctcacg cctaccgacc gcgaggcgct gaacaagctg cgaacggcgc taacccgcgg
    10141 tgctgtcatt aacagtcccc gtccccgctg aacttgcatt acccgtgtca cgctacatcg
    10201 tcggcgcatt cctgcttctc gggactctcg gcagcggctg gctgcacggg cgattggtaa
    10261 atcgctgggg gcaggcctcc ccattggcga ccgcagccgc tcggctgcag ggcggcctgc
    10321 ccgaccacct ggggcattgg cagctagtga agacacatga attggaagaa ggagtagccg
    10381 atatcttgca atgtgcagcc tatctccacg gcgtttacac aaatgaccag actggcgaca
    10441 caattgtcgt cgcacttgtc gccggtccat ccggcccgat ctcggtccac actccggaga
    10501 tttgctattc tgcgcagaac tatgagattg ccggcgaccg cgagctaatg aaagtcaggg
    10561 atttacacgg caatacgcat caggtttggc aggtgtttgc caactcgcgc gattccgcgc
    10621 ggcccaactt gcgagtcata tacggttgga gtcgcggagc aaattgggaa gcggtcgccg
    10681 gtccgcgatt tgcttatgcc gggctgcctg tactctataa gttgcaactg gccggccccg
    10741 cccgcgagca tcgttcctca caaccttccg ccgatgcctg ccaagacttt ctctccgagt
    10801 tcttggcaca gattcagcct cgactgatct ccgcccctgg cggcgactca ttcatacatc
    10861 catctgcccc gaaaggagtc ttgtgatgtc gctcacgcaa cccgccgatg ttgtctggag
    10921 tgaaaacgtc tgcccccttg ggcagaaatg taagtccgat gcggttcgcg ctataatcga
    10981 gcgcgtgatg ggactgctat tgttagtact ggtgtcacca ttaatcgttg cgctagttgt
    11041 agtcattcgc ctcacatcgc caggtccggg aatctattgt caacgacgcg ttggcctgag
    11101 gggccgcatt ttcacgatgt acaagctgcg tagcatgcgc agcgacgcgg aaaaccgctc
    11161 gggtccggtg tgggcgagcg tcgttcgcga tgcgcgtgtg acgccgctgg ggtattggtt
    11221 gcgacgtttg cacctcgatg agcttccgca gctttataac gtcgcccgag gcgagatggc
    11281 gctcgttgga ccgcgtcccg agcggccgga gtttgtccaa atcctggccg agcaaattcc
    11341 agaatatctc gaccggctgg aggtgaagcc gggcattacg ggtctggccc aaatcaattt
    11401 gccgccagat agcgacttgc agagtgttcg ccgcaagctc gtactggacc aggaatactt
    11461 acggtgcgcc ggtccacttt tcgatgcccg gatcgtgctc tgcacggccc tccgcgtgtt
    11521 tggactgcgc ggcggccggg cagtgtcgtt gatgggctta tcgaggaccg tcgaattgcc
    11581 gccggtctgc gacgctagca cgcagtcagc gccgccggtc gccgccagcg ccccggccca
    11641 accagagcac gatcggcgtc cgctctcgca ccccgcgccg ctgattgtcg ctcgcagcta
    11701 gctgacctcc gaacgccgtc ggctcccatg gacttcgtcg tatttgtgct ggtcaacgcc
    11761 gcactatttc tacggccaca ggacctgatc cctgatctgg ccgaggttcc gatctacaac
    11821 gtgctcatcg ttttcaacct cgtcgtcgcc gctcctcact tggtaaggga gctgcgctcg
    11881 gagccgcgcc gctcgcccgc cacaatctgt gtggtcgcca ttctcgttgc agctgtcctg
    11941 tcgctcctga tcaaattcga ttggcgggcc gcgtggcagg caggcttgca gttcgccaag
    12001 gtgggcgcct atttcctgct gatcactgcg gtgctgagaa gcccgcggcg gtatactacc
    12061 ttcctcgccc tcgtggtcat cttgactacc gtgttagcgg cgatcgccgt cgcccatttt
    12121 cacggccagt taaatgtcgc ggcgattacc cacgcccgcg aagtgaccta cgacgcagcc
    12181 acgggtgatc agagcagtgc gttgcggctt tcggcattcg gcgtctttgc cgaccccaac
    12241 gatttgtcga tggtggtcgt gctcagcatc ctgattggcc ttggcggaat ttgctatccc
    12301 aagctcggta tgctgcgcgt gccgctgatc ggggttgtct tgtttttggg atataccctc
    12361 gcgttgacgc agtcgcgcgg cggactcttg gcgttggtcg cgggcctcgg cgcatttgtg
    12421 ctgtgccgct atggagtcag ccggatgagc ctggcgctgg tcgcgctcgt gccgcttctc
    12481 ttcgtagtgt ttggggggcg gcaggctgat atcagcggcg gcatcgcatc aggtacgggc
    12541 agctcccgta cggacctctg gtacgccggc ctgcagatga tcaagtggca gccgctgtgg
    12601 ggtgtcgggc acggccggtt tgtggagcat gaaggcctcg tcgcgcatag ctcttacttg
    12661 caagccctgg cggagtgggg cgtcctgggc ggcgcgatgt tcgtgggatt gttttacgtc
    12721 gtgctgtatt ccatttggcg gctgcggcgt tttcgcccaa tgattggctc gggagtgctg
    12781 cgaagctttc atccgtttgt tctaggcgcg ctggcagcct atgcgacgtc catgctgacg
    12841 ctaacgcggt gcgaagtcgt gcccacgtat ctcgtcgcgg ggttgggcgt gagctacgag
    12901 cgcttggcgc gccgcaatgt gccgctgcgc ccgctggaat tcacaccgca gttgttctcc
    12961 gcaatgctcg gagtcagcct cgcttacttg gcggtcttat acgtatttat tcgcttcatc
    13021 tatcgcatgt tctagatcac ttgcgctatg tcctgtgaat ctaccacaac ctcgatccaa
    13081 catacggtga tcgagccgtc gcgcggctgg cagctcatca atttacgaga gctgtggcag
    13141 tatcgtgagt tgtttgggtt tctgatttgg cgcgatgtca aggtacgtta caagcagacc
    13201 ttgctaggag ctgcctgggc cattcttcaa ccagcgctga tgatgatcgt cttcacggtc
    13261 ttctttgcca ggctggccag agttcccacc ggcaacttgc cgtacccgtt gtttgtttac
    13321 gcgggcctgc tgccttggtt gctgttctcc acttctgttt cccaggcagc gcagagcgtc
    13381 cttagctccg agcgcctgat tactaaagtg tattttccaa ggctagcgat tccgctggca
    13441 tcggtcggcg cggccatcgt tgatttcgtc atcgccctgg tcctgctact gggcttgatg
    13501 ctttggtatg gcgttacgcc caccgcgggt ttgctcgtag tgcccttggt gatgggctgc
    13561 ttgctgctgg cggcgacagg cgtcggcacc gccattgccg cgctgaatgt ggcctaccgg
    13621 gactttcgct atctcgttcc ctttctgctg caaatgtgga tgtttgcgac tcccaccatc
    13681 tatatggacc cggcggctaa tggcgccggg agactgacga gcctgctggc acttaatcca
    13741 ctcgcacctc tggtgagcac gtttcgtgcg gccaccgttg gcgagccgat ttcctggctg
    13801 ggcctgctgc tttctgcggc cgtggccggt gtcatgttcc tggcaggctg tgcgatcttt
    13861 cggcgctttg aggacgagtt ctcggacatt atttaaagcc gtgggccaat gtatcacgct
    13921 gccgcctata ttcacgacga gatcagcctc tacaacgcct cgaaggccat ggcaggtctg
    13981 ttggagcttg cggaactcgg tgaaatcact ttgcggctga cagaccgcgg ctttgctgct
    14041 ccgcatccgg cggtgttcca gctgacagtt gccgaagatg acggcgagcc gcagcgcgct
    14101 gcgatcgact tgctcgatcg cagcgacgtg gtcgatttcg cgactttggc ttcctgcgac
    14161 gtttatttca agcgcagcta cttccaggcc gacctcgcaa aactttccca gcaatcgtgg
    14221 ctccggccgc agaccgcaaa gatccgcccc tggggtctca atttcccctg ccttgttcct
    14281 gccttgaaat cgcgccgctg gaaactcctg gcgcgctgct tctggaaacg cagcctgcgg
    14341 gcgctggtgc gcggccctca agccgcgcgt gccgttgcac aagaaatgcg gcagtttgcc
    14401 tgccttcccg ctgcggcaca attcgaagcg gatccggggg agcccttgga acccattgtg
    14461 gtgtttcaaa cacggttgtg ggagccgcac gaagcagcgc ctgacgacgc agccgcagtc
    14521 aaccggatgc gcgtggaggt ggtgcgggcg ctgcggcaag cgctgagcac cgcgtttgtc
    14581 ggcgggctgg tgagcacgcc gctcgctcgg cgcgaacatc cgggcgaggt cgtacccccg
    14641 cagcaatcgc ggatggtgaa ctacgccgcg ctcagccgcc gcgccctcat cggggtctac
    14701 acccgcggct tgcaccactc gacggccttc aagctgcctg agtatctcgc cggctcgaag
    14761 gcggttgtcg ccgaggggct gcgaaaccag acgcctaccc cgctggaacg gggattgcac
    14821 tgggccgagt tccgcgatgc cgacgagtgc gttctggagt gccgccggtt gctcgccgac
    14881 cacgaacgct gcgcggcgat gcgccaggcg tcggccgaat actaccgtct ccatgtcgca
    14941 ccgcaagctc acctccgctg gaccttggcg gaagcctgcg ccgcgcgccc cgcctcagcg
    15001 gccgctccgc ggcttgttac gcaagaacca gagttagcca ggtcgctatg actgccgccg
    15061 tccgaatcga aaacctcagc aagcggtatt tcgtcgatca cgagaatcgc agccacgtca
    15121 gctatcgcac gctgcgcgag agtttggcgc atcttgccgc aacgccgctg cggcggctgc
    15181 agggccagcg ccgcgcaacg cgcgaagaat tctgggcctt gcgagaagtc agcttggagg
    15241 tggaagcggg cgaagttgtc ggcatcatcg gccgcaacgg cgcgggcaag agcacgttgc
    15301 tgaagattct gagccgaatc acgaaaccga cgcgcgggcg cgtggaactg actggcaggg
    15361 tcggcagcct gcttgaggtc ggcacgggct ttcatcccga gttgaccggc cgcgagaacg
    15421 tgtatctcaa cggcagcatc ctggggatgc agaagcggga gatcgacgcc cgcttcgatc
    15481 agattgtgga cttcgccgag atcgagcaat tcctcgatac cccggtgaag cgctacagca
    15541 gcggcatgta cgtgcggctg gcgtttgcga tcgccgcgca cctgcacccg gaagtgctca
    15601 tcatcgacga agtgctggcc gttggtgaca ccgcattcca gcgcaagtgt ttggggagca
    15661 tggagtcgct gggcaaatcc gggcgcacgg tccttttcgt cagccacgac ctgaacgcgg
    15721 tggccaaact caccacccgc tcgttcctgc tggagcgcgg ccggctgacc gccagcggca
    15781 acacgccgga cgtcattcac tcctacctgc gctgttccga aggatactcg tcggagtggt
    15841 gccgccaggg gccgccgcac gcggagcggc cgacgatcct gaggatcgtg gcactcaatc
    15901 cagacggcca gcggtgctcg ctgttcgacg ccgatcagcc gttcgagatc gagtttcagt
    15961 gccattttcc gcgggcggtg gaagcacaac tggccttccg gctgaacgcg gatttccaga
    16021 ccgagacgat ctttacgagc agcctggccg acggcgagga ccgcgcgacg cggattggcg
    16081 aagggctggt gacggcgcgg tgcaccgtgc ccgctcacct gctcataccc gggcggtacc
    16141 acgtactcgc agcgattaac aacttcaagg ggccgcagtt cgacatggtc gaacgggcac
    16201 tggccttcga ggtcaccgaa atcggttcgc tgaccaaaaa cgacggacgc ctgggaaagg
    16261 tcgcgccgtt gttggaatgg acctgcaccc aaggggcggc cccacccgcg ctcgccacgc
    16321 atccatgctg acagcacccc ttacgtcgat ttatcgcacc ctcttgccgc cgcaagtgcg
    16381 cgctccgctg tggcacctgc gctcggcggg cggggccctg ctgtcggatc ggccgagcta
    16441 ccggcaatgg ctgctggact tttaccgcag tatcggcagg tttcggcccg ttgtgcgaac
    16501 ggcgatcggc acgcgtctgg ccgtcgatct gcgcgacgag ggcatcggtt ggccgatctt
    16561 cgtgcaccag gagtatgaac cgggggaaac ccgtttcatt gcgcggacgc tgcgggccgg
    16621 cgacgtcttt gtcgatgtcg gcgccaatgt cggatacttc acgacgctcg ccagcgggcg
    16681 ggtcggcccg cgcgga
//
LOCUS       DASRJB010000679         5797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_764724, whole genome shotgun sequence.
ACCESSION   DASRJB010000679 DASRJB010000000
VERSION     DASRJB010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764724"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..769)
                     /locus_tag="VFB80_18075"
     CDS             complement(137..769)
                     /locus_tag="VFB80_18075"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN35746.1"
                     /translation="MASQVAAAPGAPISAPLNRTEQVIERLVRRRVAVSLLLFAGLIV
                     LDLFVFRSRPRDVLQLRDPLVAVSDALIALGLLIRSWAAGTLRKKKHLATTGPYAWIR
                     NPLYFGSFLMMVGFAILVQDPLTLWIVIGPLAWLYWQAVRSEERQMAEIFPDEWPEYA
                     AAVPRFIPRRFVWPLAGEWSLAQWRHNLEWQAWLGSAGALLGTKLWLNAL"
     gene            complement(972..2384)
                     /locus_tag="VFB80_18080"
     CDS             complement(972..2384)
                     /locus_tag="VFB80_18080"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZN35747.1"
                     /translation="MRQFLFDYHGTELTYLAAAALFILSLFWMNSPQTARRSVLAGVL
                     AMGLAMLGAALDPSVVRWGWIVTAIVLGCAVGVPLSWVPLTAVPQRTALSHAFGGLAV
                     GLLGTAKFYDWLANDPAKLTQFTMAAIVAEVLLGFLTFTGSLMAAAKLQEIKWIPQRP
                     VTYPFQNVANFALLGVALLCGLALTISPGGTYLLFPLIIILALAFGVLLVLPIGGADM
                     PTVISLLNSYAGLSAVALGFVLDNKLLITAGALDGSSGLILSIIMCRAMNRSFTNVLF
                     GAFGQVQTKAAGGEARTVKSETPEGAAQIMEQANLVVIIPGYGMAVAQAQHRVKELYD
                     LLTKRGVDVRFAIHPVAGRMPGHMNVLLAESEIPYDKLVEMDDINHDLPQADVALVIG
                     ANDVVNPAARHDKSSPIFGMPIIDADKARTCFAIKRSMNPGFAGIENELYYADRTYML
                     FGDAKAVIGDLVKALSGGGH"
     gene            complement(2381..2794)
                     /locus_tag="VFB80_18085"
     CDS             complement(2381..2794)
                     /locus_tag="VFB80_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZN35748.1"
                     /translation="MTHPTVLALFATLLLASPLYADPVAPAGHATKPAADMPTLLFVF
                     MLATFIGLGVITRVSRLLHTPLMSLTNAISAIAVVGAIAVAGHEHVTKAGTQSITLNV
                     LGAIALFASMTNIVSGFLLTDRMVKMFKSQEGAKP"
     gene            complement(2980..4158)
                     /locus_tag="VFB80_18090"
     CDS             complement(2980..4158)
                     /locus_tag="VFB80_18090"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZN35749.1"
                     /translation="MQVGIPKETYPGERRVALVPADVAKLAKNGIGVILEPGAGAAAG
                     FPDAAYEEKGAKLAFSREGAFSGEIVAQVRAGGANAAGGQADLQWLRPGKIVVAGVDP
                     LGAPQEVAEIAKTGATLFALELMPRTTRAQAMDILSSMANLAGYRAVLLAAAELPKIF
                     PLMMTAAGTLSASKVFVIGVGVAGLSAIATAKRLGAVVRAYDVRPACREQVESLGAKF
                     IELPLDTKQAEAAGGYAKAQDEAFYQRQRELLANIVADSDAVITTAAIQGKPSPLLVT
                     AEAVQRMQPGSVVVDLAAERGGNCALTKADERVSQFGVTILGPTNLPSDLAYHASQMF
                     SGNVTAFLLNLVRKGQVVLNCDDEIIRETLAAHEGQVAHPRLRDLLGLPKLPQETLET
                     "
     gene            complement(4292..4396)
                     /locus_tag="VFB80_18095"
     CDS             complement(4292..4396)
                     /locus_tag="VFB80_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZN35750.1"
                     /translation="MLTYLRQTDKRLGLVINFGEILVKDGIHRVVNKL"
     gene            complement(4481..>5797)
                     /locus_tag="VFB80_18100"
     CDS             complement(4481..>5797)
                     /locus_tag="VFB80_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN35751.1"
                     /translation="PNLDRMAAEGARFTCGYCSQPICSPSRAGLMTGRFPARLHITTF
                     LPGRPDAKSQMVLHPKIEMQLPLAEKTIAEYLKEAGYATGCVGKWHLGGMGFGPKEQG
                     FDFAFAGRANTEPSATEGGKGEYEHTAQALKFIDEHKEQPFFLYVPYNTPHIPLAAQE
                     KLIAKHKDAFYPTYAAMMETMDDCVGLILKKLKDLGLDEKTLVIFTSDNGGLSVLEFA
                     GGPSTHNTPYRGGKGYLYEGGLREPTIVRWPGQIRPAVIDAPIINLDLLPTLLSLAGV
                     TIPTNLDGQNIAPLLLGTEENKELRSRTFYWHFPHYNNQGGRPAGALRDGDLKLVTYY
                     DTGETQLFNLKDDPGETKDLAPQQSPLAKGLKATHDAWLDLIGAQRNVPNPEFDPALF
                     KKLYQDFDPSRPILRKTTVEMEQDMKTWRQLMNEVVAGARGKNKKK"
BASE COUNT         1010 a         1909 c         1875 g         1003 t
ORIGIN      
        1 ccgtgcccaa gatccgcaac tttctgaacg acctgcacca gaaagcggcg atccgttacg
       61 tcatcacggc cgagacgggc gagcaggacg ttttgctggt ggacggccag cgccccaagc
      121 cggcgtcgta gcgggcttag agcgcgttca gccagagctt cgtgcccaag agggccccgg
      181 cggagccgag ccaggcttgc cactcgagat tgtgccgcca ttgggccagc gaccactccc
      241 cggcaagcgg ccagacgaac cggcggggga tgaaccgcgg cacggcggct gcatactcgg
      301 gccattcgtc ggggaagatt tcggccattt gccgctcttc cgaccgcacg gcctgccagt
      361 aaagccaggc cagaggcccg atcacgatcc agagcgtcag cgggtcctgc acgaggatgg
      421 cgaagccgac catcatcaaa aacgagccga agtagagcgg gttgcgaatc caggcgtagg
      481 ggcccgtcgt cgccaggtgc ttcttcttgc ggagcgtgcc cgcggcccag ctgcggatca
      541 aaaggcccaa ggcgatcagc gcgtcggaga cggcgaccag cggatctctg agctgcagca
      601 cgtcgcgcgg gcgactgcgg aacacgaaca ggtcgagcac gatcagcccc gcgaagagca
      661 gcaagctcac cgccacccgc cgccgcacca ggcgctcgat cacctgctcg gtgcggttca
      721 gcggcgcgga gatcggcgca cccggggcag ccgccacttg tgatgccatc acagcgtccg
      781 aaacgcaggg aggtaagtcg gggtagctcg cgaagactgg cagcagccaa ttcgccactc
      841 ttttatcgtc ctgcgccgga accggcaatg acaacttgcg taagacaacc gaaataggcg
      901 tcaagctcgg caagcgaggg agagtcttcg cccgcggcct tccgcaagcg gtcgccagcg
      961 agcggtgcta gctagtgccc gccgccgctg agggctttca cgaggtcgcc gatgacggct
     1021 ttggcgtcgc cgaagagcat gtaggtgcgg tcggcgtagt acagctcgtt ctcgatgccg
     1081 gcgaagccgg ggttcatgct ccgcttgatg gcgaagcacg tgcgggcctt gtcggcgtcg
     1141 atgatcggca tgccgaagat cgggctcgac ttgtcgtggc gggcggcggg gttcacgacg
     1201 tcgttggccc cgatcaccag ggccacgtcg gcctgcggca ggtcgtggtt gatgtcgtcc
     1261 atctcgacga gcttgtcgta aggaatctcc gactcggcca gcagcacgtt catgtggccg
     1321 ggcatgcggc cggcaacggg gtgaatggcg aatcgcacgt ccacgccgcg cttggtcaag
     1381 aggtcgtaca gctccttgac gcgatgctgg gcctgggcga ccgccatgcc gtagccgggg
     1441 atgatgacga cgaggttggc ctgctccatg atctgggcgg ccccttcggg cgtctcgctc
     1501 ttgactgtcc gggcctcgcc gccagcggcc ttggtctgca cctggccgaa cgctccgaac
     1561 agcacgttgg tgaacgagcg gttcatcgcc cggcacatga tgatcgacag gatcaggccg
     1621 ctggagccgt cgagcgcccc cgcggtgatc agcagcttgt tgtcgagcac gaagccgagg
     1681 gccaccgccg agaggcccgc gtaggagttg agcagcgaaa tgacggtcgg catgtccgcg
     1741 ccgccgatcg gcagcaccag cagcacgccg aacgccagcg ccaggatgat gatcagcggg
     1801 aacaagaggt acgtgccgcc cgggctgata gtcagcgcca ggccgcacag cagcgccacg
     1861 cccaagagcg cgaagttggc cacgttctga aacggatagg tcaccggccg ctgcggaatc
     1921 cacttgatct cttgcaattt cgcggcagcc atcaggctgc ccgtgaacgt caagaaacct
     1981 aggagcactt cagccacgat ggccgccatc gtgaattgcg tcagcttcgc cgggtcgttg
     2041 gccagccagt cgtagaactt ggccgtgccc aacaggccga cggcgagccc gccgaacgcg
     2101 tgcgacaggg cggtccgctg cggcacggcg gtcagcggca cccacgacag cggcacgccg
     2161 accgcacagc ccagcacgat cgccgtcacg atccagcccc agcggacgac gctcggatcg
     2221 agggccgcgc cgagcatcgc caggcccatc gccagcacgc ccgccagcac cgaccggcgg
     2281 gccgtctgcg ggctgttcat ccagaacagc gagaggatga acagcgccgc cgccgccagg
     2341 taggtcagct cggtgccgtg gtagtcgaac agaaactgcc tcacggcttg gcgccctcct
     2401 ggctcttgaa catcttcacc atccggtcgg tgagcaaaaa gccgctgacg atgttcgtca
     2461 tcgaggcgaa cagggcgatc gcgcccagga cgttcaacgt gattgactgt gtgccggcct
     2521 tggtcacgtg ctcgtggccg gcgacggcga tcgccccgac gactgcaatc gccgagatgg
     2581 cgtttgtcag cgacatcagc ggcgtgtgca agagccgcga gacccgcgtg atcacgccca
     2641 gcccgatgaa cgtcgccagc atgaatacga acagcagcgt cggcatatcc gccgccggct
     2701 tcgtcgcatg ccccgccggc gccaccggat cggcatagag cggacttgcc agaagcagcg
     2761 tcgcgaacag tgccagcacc gtagggtggg tcaagcgagt ccgcgagcgc agacccacgc
     2821 ggcggtggag ccaccctccg cgtgggtctt cggccgcagc ggcctcgacc caccctacat
     2881 ccgagtactg agtacggagt acggagtacc aagtacgaat caaaccacga caaattcggc
     2941 caatcttcct cgtcactaac tgtctcctgt ccccctggcc taggtctcca gcgtctcctg
     3001 cggcagcttc ggcaggccga gcaggtcccg cagccgcgga tgggcgacct ggccctcgtg
     3061 ggcggcgagc gtctcgcgga tgatctcgtc gtcgcagttg agcacgacct gcccctttct
     3121 gaccaggttc agcagaaacg ccgtcacgtt gcccgagaac atctggctgg catgataggc
     3181 caagtcgctg ggcaggttcg tcggcccgag aatcgtcacg ccgaactggc tcacccgctc
     3241 gtcggccttc gtcagggcgc agttgccgcc tcgttcggcc gccagatcga caaccaccga
     3301 tcccggctgc atccgctgca cggcttcggc ggtgaccaag agcggcgaag gtttgccctg
     3361 aatcgcggcg gtggtgatca cggcatcgct atcggcgacg atgtttgcca agagctcgcg
     3421 ctgccgctga tagaaggcct cgtcctgggc cttggcataa ccgccggctg cttcggcctg
     3481 cttcgtgtcg agcggcagct cgatgaactt ggctccgaga ctttcgacct gctcgcggca
     3541 cgccggacgg acgtcgtagg cccgcaccac cgcacccagg cgcttggccg tggcgatggc
     3601 cgagagcccc gcgacgccga ctccgatcac aaacaccttc gacgccgaga gcgttcccgc
     3661 ggcggtcatc atcagcggga agatcttcgg cagctcggcc gccgccagca gcaccgcgcg
     3721 gtatcccgcc aggttggcca tcgacgagag gatgtccatc gcctgggccc gcgtcgtccg
     3781 tggcatcagc tccagcgcga acagcgtcgc gccggtcttg gcgatttcgg cgacttcctg
     3841 cggcgctccc agcggatcga cgccggcgac gacgatcttg ccgggcctca gccactgcaa
     3901 atccgcctgg ccgccggccg cgttcgcccc gccggcccgc acctgggcca cgatctcgcc
     3961 cgaaaaagct ccctcgcgcg agaaggccag cttcgcgcct ttctcttcat aagccgcgtc
     4021 gggaaacccg gccgccgctc cggcccccgg ctccaggatc acgccgattc cgtttttcgc
     4081 cagcttggcc acatccgccg ggacaagcgc cacgcgccgc tcgcccggat aggtctcttt
     4141 gggaatgcca acttgcatgt caagtctggg gagcagtcgc gaaaaaaacg cgcggcctgt
     4201 ggtgcggggc tcgtcctgtt taacgccaag gcgcaaaagg ggcaaagacg caaagcagca
     4261 ctaggaggcg tgggaaagtg taccttgcgg ttcagagttt gttgacgacg cggtgaattc
     4321 catctttgac gagaatctcg ccaaagttga tgaccaggcc cagtcgtttg tccgtttggc
     4381 ggagatacgt cagcaattcc tagttgatct ctctatctgc tttgcgtctt gccgccttcg
     4441 ccccttggcg ttaaatcgag cgacgctgcc ccgccagaga ttacttcttc ttgtttttcc
     4501 cccgcgcgcc ggcgacgact tcgttcatga gctgccgcca ggttttcatg tcctgctcca
     4561 tttccacggt ggtcttccgc aggatcggcc gggagggatc gaagtcctgg tagagctttt
     4621 tgaacagcgc cggatcgaat tcggggttgg gcacattccg ctgcgcaccg atcaggtcga
     4681 gccaggcgtc gtgtgtggcc ttgagtccct tggcgagtgg cgactgctgg ggggcgaggt
     4741 ccttcgtttc gccggggtcg tctttgagat tgaagagctg cgtctcgcca gtgtcgtagt
     4801 aggtcaccag cttcaaatcg ccgtcgcgga gtgcgccggc ggggcggccg ccctggttgt
     4861 tgtagtgcgg gaagtgccag tagaacgtcc gcgaccggag ctccttgttc tcttcggttc
     4921 ccaggagcag cggcgcgatg ttctggccgt cgaggttggt cgggatcgtt acgccggcca
     4981 acgacaacag cgtgggcaga aggtcgaggt tgatgatcgg cgcgtcgatc acggcgggcc
     5041 tgatctggcc tggccagcgg acgatcgtcg gctcgcggag gccgccttca tagagatagc
     5101 ccttgccgcc gcggtagggc gtgttgtgcg tcgagggccc gccggcgaac tccagcaccg
     5161 agagcccgcc gttgtcggac gtgaaaatga ccagcgtctt ctcgtcgagg cccaggtcct
     5221 tgagcttctt caggatcagc ccgacgcagt cgtccatcgt ctccatcatg gccgcgtagg
     5281 tcggatagaa cgcatccttg tgcttggcga tcagcttctc ttgggccgcc agcggaatgt
     5341 gcggcgtgtt gtagggcacg tagaggaaaa acggctgctc cttgtgctcg tcgatgaact
     5401 tcagcgcctg cgccgtgtgc tcgtattccc ccttgccgcc ttcggtcgcc gacggttccg
     5461 tgttcgcccg cccggcaaag gcgaaatcaa agccctgctc ttttggccca aaacccatgc
     5521 cgcccaagtg ccacttgcct acgcagcccg tggcgtagcc agcttccttc aaatactcgg
     5581 cgatcgtctt ttcggcgagc ggcagctgca tctcgatctt gggatgcagc accatctgcg
     5641 acttcgcgtc gggccggccc ggcaggaacg ttgtgatgtg cagccgggcc gggaaccgcc
     5701 ccgtcatcag cccggcgcgc gacggcgagc agatcggctg cgagcaatag ccgcaggtga
     5761 accgcgctcc ctccgccgcc atgcggtcca gattcgg
//
LOCUS       DASRJB010000680         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2740156, whole genome shotgun sequence.
ACCESSION   DASRJB010000680 DASRJB010000000
VERSION     DASRJB010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740156"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFB80_18105"
     CDS             <1..471
                     /locus_tag="VFB80_18105"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35752.1"
                     /translation="LNRPLSETSCERRDETTVTPQVFALFNGEFAANRALAMAARLTK
                     EHSSGAERITAAFRTVYGREPTAAESERCLTHLTRMTAHHEAHPPTPSELPTKIRRGM
                     VEELTGEMVYWDEDLSVLKDYQRDLMPWQVEPATRALADICLVLLNSNEFLYLR"
     gene            559..1925
                     /locus_tag="VFB80_18110"
                     /pseudo
     CDS             559..1925
                     /locus_tag="VFB80_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
     gene            complement(1934..>2341)
                     /locus_tag="VFB80_18115"
     CDS             complement(1934..>2341)
                     /locus_tag="VFB80_18115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN35753.1"
                     /translation="YTLSALLAADYDAAIALWNSCEGVRANETREEFARILERNPGLS
                     GAIRCGGELAAAVLCCHDGRRGYLYHLGVAQEHRQHGLGRALVEHSLAQLRAAGIRRC
                     TIFLITSNAGGEAFWKRIGWRDRDDLKVLAYDL"
BASE COUNT          426 a          786 c          747 g          382 t
ORIGIN      
        1 ctcaaccggc cgttgagcga gacgagctgc gaacgccgcg acgagacgac cgtcacgcca
       61 caggtctttg ccctgttcaa cggcgaattc gccgccaacc gggcgttagc gatggcggcc
      121 cgcctgacga aggagcattc ctcgggcgca gagcgaatca ccgccgcgtt tcgcacggtc
      181 tacggccgcg agccgaccgc cgccgagagc gaaaggtgcc tgacccatct gaccaggatg
      241 accgcgcacc acgaggccca tccgcccaca ccgtccgaac tgccgacgaa gatccgccgc
      301 ggcatggtcg aggagctaac gggcgagatg gtctattggg acgaagacct gtcggtcctc
      361 aaagactacc agcgcgacct catgccctgg caagtcgagc cggccacgcg ggcgctggcg
      421 gatatttgcc ttgtgctttt gaattcgaac gagtttttgt atctgcgcta ggaatcgagt
      481 atggagtgcg aaggacgacg gaccgaggat caagtaccaa gtactccgta ctcagtactc
      541 ggtacgcgcc gcgatctact gtatagcctg ggattgtcgc tcggctcagc ggcgcttagt
      601 tcgctcttgc aagccgaaga tcgtccggcc ggtcctctgt cgcccaaacc gcctcatcat
      661 gccgccaagg ccaaggcctg catctgcctg ttcatggagg gcgggccgag ccacatcgac
      721 acgttcgatc ccaagccgaa gctggccgag ctgcatatgc aggaattcac gcggaacgac
      781 aagttcgcgt cgaacatggg cagcggcaag cgatactaca tcgccagtcc gttttcatcg
      841 cggcaggtgg gcaggccgga ctttggatga gccagccgtt cgagcgtctg ccggcggtcg
      901 ccgacgagct gtgcgtctat cacgggctgc aagtcgattc ggttgatcat ccgacggcct
      961 gctaccagat gaacacgggc aaccgctttg gcggcgatcc cgcgctgggc gcttggacct
     1021 gctacgggct gggttcgccg agccagaacc ttccagcgtt cgtcgtgttg cccgaaggag
     1081 cttttccgca aggcggcgca gccaactggt cgaatggttt tctgcccgct tattaccagg
     1141 gcacgccgct gcggccgtcc ggctcgccaa ttctcgacct cgtgcctgct tacggccaga
     1201 cgcccgcggc ccagcgggcg aacctcgacc tcctggcgga gctgaaccgc gccgatctgg
     1261 cccggcatcc agagcacgcc gaggccgcgg cgcgaatgga agcctacgag ctggccttcc
     1321 gcatgcaggc ggaagtgccg gacgtcatcg atctctcgaa ggaaccgcag gcgacgctcg
     1381 atttgtacgg catcgggcag ccggcgaccg acagctttgg ccgccgctgc ctcttggccc
     1441 gcaagctggt gcaagcgggc gtgcgattcg tgcagctctt cgagggcggc tgggactcac
     1501 acgactacat cgagcgagcc cacacgcaac ggatcaaggc gatcgacaag cccgtcgcgg
     1561 cgctcgtgca agacctcaag cggctgggcc tgctcgagag cacgctcgtc gtttggggcg
     1621 gcgagttcgg ccgctcgccc gacaacggcg tccgcggcgg cgggcaggta gtcggccgcg
     1681 accacaacgc taaggcgatg aacatcctgc tggccggcgg cggcgtcaaa gccggctgcc
     1741 gcgtaggcgc gaccgacgag ctgggggacc aggccgccct gtgcgtgcac catgtccgcg
     1801 acctgcacgt gacgctgctg cacttgctcg gcctctcaga caacaagctg acctactttc
     1861 acgagggccg cttcaagcag ctcagccaga tcggcggcca ggtgatccgc gagctgctgg
     1921 cgtgatcgag tcgctacaga tcgtaggcca gcaccttgag atcatcgcgg tcgcgccagc
     1981 cgatgcgctt ccagaaggcc tcgccgccgg cgttgctggt gatcaagaag atcgtgcagc
     2041 ggcggatgcc ggccgcgcgg agctgcgcga gcgaatgctc gaccagcgcc cggcccaacc
     2101 cgtgttgccg atgctcctgc gcgacgccca agtgatagag atatccgcgc cgcccatcgt
     2161 gacagcagag cacggccgcg gccagctcgc cgccgcagcg aattgcgccg gacagccccg
     2221 gattccgctc caggatccgc gcaaactcct cccgcgtctc attcgcccgc acgccctcgc
     2281 aggagttcca caaggcaatc gccgcgtcgt aatcggcggc caaaagtgcg ctaagcgtgt
     2341 a
//
LOCUS       DASRJB010000681         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1612078, whole genome shotgun sequence.
ACCESSION   DASRJB010000681 DASRJB010000000
VERSION     DASRJB010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612078"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFB80_18120"
     CDS             <1..802
                     /locus_tag="VFB80_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35754.1"
                     /translation="ILRVTQETPTSLRNRVNFLKEVYSKYQRAKRGLSEGNLRLVVSI
                     AKKYRNRGLSFLDLIQEGNAGLMRAVDKFEYRRGFKFCTYATWWIRQAITRAVADQSR
                     TIRIPVHMVETMSRVRNVSRQLLQELGREPTLEETARKARTTVDEARRVLAMSRYPIS
                     LDRPVGNSEDSHFGDLLPDGTAESPATGASQEMLRNRINKVLKTLSYREREIIKLRYG
                     LGDGYSYTLEEVGHIFKVTRERIRQIEAKAVRKLQQPSRSQELVGFLD"
     gene            1036..1542
                     /locus_tag="VFB80_18125"
     CDS             1036..1542
                     /locus_tag="VFB80_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35755.1"
                     /translation="MLVPYHQQPAPVCALCGSASATVQGLVILKRWDEVIRIFRNRQA
                     EMEEDAREVALPLCLAHRSYRRWWPYSAMFLFFWPVVALLFFSPLLVQWWSFATACVA
                     ALIVAINFAVFVWFVRSSLRVYQLASTRHGVVLGGLAPAFVQAIRQLKQADTERVKGR
                     LDDFFRQL"
     gene            complement(1583..2644)
                     /locus_tag="VFB80_18130"
     CDS             complement(1583..2644)
                     /locus_tag="VFB80_18130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HZN35756.1"
                     /translation="MQTAITASAAVRVHGLGDLDSGGNEAASSPAVAAALADAKRWLR
                     VLHSALGHSPYLLCAEEGQQNTGVLPLAVVQSALFGRFLVSLPYVNSAGIVADSAAAA
                     GRLLDRAVQLADELNVRYLELRNEQQSTHASLEHKNDSKVLMRLPLPATPEKLLASFK
                     SKLRSQIRSGEKHNFDVRFGGRELLADFYAVFSRNMRDLGTPVYSRRLFEAILELFAE
                     QAELCVLRLAERPVAAALLLHHAETTEVPSASSLRAYNSTNANMVMYWHLLKRAVERG
                     QAVFDFGRSTVDSGTYRFKEQWGAEPSPSVWQYYLRRGSIGDLRPDNARFSLAIRAWQ
                     RLPVWLTRLIGPPIVRGIP"
     gene            complement(2647..3729)
                     /locus_tag="VFB80_18135"
     CDS             complement(2647..3729)
                     /locus_tag="VFB80_18135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN35757.1"
                     /translation="MTGNRLAFIDWLKCLGMVLIVLGHTGAQNYVDPTPPFNFKQLGV
                     AFFVFVMGFSLARERRPTPLVLYHRLFEIWLLGWLGALVLSAISWLTIRDLNESNYLP
                     LALGANVLFNHFPANPTTWYIGTYFHLLLLWACVLRPIRVRWWMVAASAVAEILIRAA
                     LMHAAGGFVAYMLLTNWTTLLLLGMWIGQQTDVRPSAAAGAIAAAALAVLAIAWPLTV
                     TRIGLTDGFPFARLNLPTPALSLLATSAAVTGLYLACTLLAHRMALQLPESSVVRFFA
                     DNTLIVFIAHMPLIYAVTPGLYPLVPAGPLGLVRMLTNLALFFVLPALCSALIRRLVQ
                     PKLLRDWLLNRLTAAWCGAAVSKSAA"
BASE COUNT          729 a         1173 c         1225 g          627 t
ORIGIN      
        1 catcctgcgg gtcacccagg agacgccgac cagcctgcgg aaccgcgtga acttcctcaa
       61 ggaagtttac tcgaagtacc agcgggccaa gcgcggcctg agcgagggca acctgcggct
      121 ggtcgtgtcg atcgccaaga agtaccgcaa ccgcggcctg tcgttcctcg acctcatcca
      181 ggaaggcaac gccggtctga tgcgggcggt cgacaagttc gagtatcgcc gcgggttcaa
      241 gttctgcacc tacgccacct ggtggattcg ccaggcgatc acgcgggccg tcgccgatca
      301 gagccgtacg attcgcattc cggtccacat ggtcgagacg atgagccgcg tgcgaaacgt
      361 ctcgcggcaa cttttgcagg agctgggccg cgagccgacg ctggaagaaa ccgcccgcaa
      421 ggctcgcacc actgtcgatg aagcccgccg cgttctggcg atgagccgct atccgatcag
      481 tctcgatcgg cccgtcggca acagcgagga cagccacttc ggcgacctcc ttcccgacgg
      541 caccgccgag agcccggcca ccggcgcatc gcaagagatg ctgcggaacc gcatcaacaa
      601 ggtgctcaag accctaagct accgcgagcg ggagatcatc aagctgcggt acggcctggg
      661 cgacggctac agctacacgc tggaagaagt cggccacatc ttcaaggtca cccgcgagcg
      721 gattcgccag atcgaggcca aagccgtccg caagctccag caacccagcc gcagccagga
      781 gctggtcggc ttcctcgact agccgttgac gaccaggctg tcagcattgc ctacgtttga
      841 atgacgtagg catggtcgtt tcttgacaat tggaacaagg caagagcata ctccgcagcg
      901 gctgaatcgt tgcggtgcag aattgccttg cgcggagtgt gcagcgtgga accagagatc
      961 aatccgtacc aaccgccagc tgaggcagcg gagattaccg gccccgccgc gcgatgggaa
     1021 aacgccgaca acgccgtgct ggtgccctac caccagcagc cggcgcccgt gtgtgcgctt
     1081 tgcggctcgg cgtcggcgac agtgcagggc ctggtaatct tgaagcggtg ggatgaggtg
     1141 atccggattt tccgaaaccg gcaagccgaa atggaggaag atgcacgaga ggtagcgctg
     1201 ccgctatgtc tggctcatcg aagctatcgg cgctggtggc cctactcggc catgtttctg
     1261 ttcttctggc ccgtggtggc cctgctcttt ttttcgccgc tgctagttca gtggtggtca
     1321 ttcgccacgg cctgcgtagc ggcactcatt gtcgcgatca actttgcggt cttcgtttgg
     1381 ttcgtcagaa gctcgcttcg ggtctatcaa ttggcgtcaa ctcgccacgg cgttgtgttg
     1441 ggcggcttgg ctccagcatt cgttcaggcg attcgccaat tgaaacaggc tgataccgag
     1501 cgcgtgaagg gacgattgga cgacttcttt cggcagcttt agcgaaaaaa tgcgagcgcg
     1561 agacagaaga gttcaccgcg ggctacggaa ttccgcgaac gatcggcggg ccgatgagcc
     1621 gcgtgagcca gacgggcagc cgttgccagg ctcggatcgc caggctgaac ctggcgttgt
     1681 cgggccgcag gtcgccgatc gacccgcggc gcaggtaata ctgccagacc gacgggctcg
     1741 gctcggcacc ccactgctct ttgaagcggt aggtgccgct atcgaccgtg ctgcggccga
     1801 agtcgaacac ggcctggccg cgctccacgg cgcgcttcag caggtgccag tacatgacca
     1861 tgttcgcatt agtcgagttg tacgcccgca gcgagctggc gctggggact tccgtcgtct
     1921 ctgcatggtg cagtaagagc gctgcggcga cgggccgctc cgccagccgc aagacacaca
     1981 gctcggcttg ctcggcgaag agttcgagaa tcgcctcgaa caatcgccgc gaatagaccg
     2041 gcgtgcccag gtcgcgcatg ttgcggctga agacggcgta gaaatcggcc agcagctcgc
     2101 ggccgccgaa acgtacgtcg aagttgtgct tttcgccgct gcgaatctgg ctgcgcagct
     2161 tcgatttgaa gctggccagc agtttttccg gcgtcgccgg cagcggcagg cgcatcagga
     2221 ccttggagtc gttcttgtgc tcaagcgaag cgtgcgtcga ttgctgctca ttccgcagct
     2281 ccaggtagcg cacgttgagc tcgtcggcga gctggacagc ccggtccagc agccggccgg
     2341 cggctgcggc gctgtcggcc acaattcccg ccgagttgac atagggcagg ctgaccagga
     2401 accggccgaa aagcgcgctt tgaactacgg ccagcggcag cacgcctgtg ttctgctgcc
     2461 cctcttcggc gcagagcaga taggggctat gtcccagcgc cgagtgcaga acgcggagcc
     2521 agcgcttggc gtcggccagc gccgcggcga cggctggcga gctggcggct tcgtttccac
     2581 cggagtcgag atcgccgagg ccgtggacgc gaacggcggc ggacgcggta atcgcggttt
     2641 gcattgtcac gccgccgact tggagactgc agcgccgcac caggctgctg tcagacggtt
     2701 cagcagccag tcgcgcagca gcttcggctg cacgagccgg cgaatcagag cggagcagag
     2761 cgccgggaga acgaagaaca gcgccaggtt ggtgagcatc cgcacgaggc ccaacggccc
     2821 cgcgggcacg agtgggtaca ggccaggcgt cacggcatag atcaacggca tgtgggcaat
     2881 gaaaacgatc agcgtgttgt cggcgaagaa gcggacgacg cttgactcag gcagctgcag
     2941 cgccatgcga tgcgcgagca gagtgcaggc gagatacagg cccgtaacgg cggccgaagt
     3001 cgccagcagc gagagtgcgg gcgtcggcag gttcagccgg gcaaacggaa agccgtctgt
     3061 caggccgatt cgtgtgacgg tcagcggcca ggcgatcgcc agcactgcga gggccgccgc
     3121 ggcgatcgcg ccggctgcag cgctgggccg aacatccgtt tgctggccga tccacattcc
     3181 caatagcagg agcgtcgtcc aattcgtcag cagcatgtac gccacgaacc cgccggccgc
     3241 atgcattagc gctgcgcgga tcaggatttc cgccacggcg ctggcggcga ccatccacca
     3301 tcgcacgcgg atcggccgca gcacgcaggc ccaaagcagc agcaaatgaa agtacgtccc
     3361 gatgtaccac gtcgtcggat tggccggaaa gtgattgaag agcacgtttg cgccgagcgc
     3421 cagcggcagg tagttgcttt cgttcaggtc gcgaatcgtc agccagctga tcgcgctcag
     3481 cacgagcgca cccagccagc ccaacagcca gatctcgaac agccggtggt agagcaccag
     3541 cggcgttggc cgccgctcgc gagccagcga gaagcccatg acgaagacaa agaacgcgac
     3601 gcccagctgc ttgaagttga atggcggcgt cggatcgaca tagttttgcg ccccggtgtg
     3661 tcccagcacg atcagcacca tgcccaggca cttgagccaa tcgatgaatg ccaggcgatt
     3721 gccggtcatg cctcaaccgc caccggctgc ggtc
//
LOCUS       DASRJB010000682         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2456694, whole genome shotgun sequence.
ACCESSION   DASRJB010000682 DASRJB010000000
VERSION     DASRJB010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456694"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="VFB80_18140"
     CDS             complement(<1..2195)
                     /locus_tag="VFB80_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN35758.1"
                     /translation="MAFRFDKLTVKSQEAVQRAQSLAADKGQAEMEPLHLLAAMLEDA
                     EGVARPVLEKVGVNVQQMLRQVRAELDRRPQVSGGTQPNPNRQLMAVLEGALQEASAM
                     KDEFVSVEHLLLALTKVDSPAKSLLQVNGVREKDVLEALKSVRGSARVTDQNPEGKYQ
                     ALKKYGIDLVEKATAGKLDPVIGRDQEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVQGDVPQSLKNRRVIALDMGLLVAGAQYRGQFEERLKAVIREVQESNGQVIL
                     FIDEMHTVVGAGKAEGAADAAQLLKPALARGELRCIGATTLDEYRQHVEKDAALERRF
                     QPVYVGEPNVEDTISILRGLKPRYEAHHGVKIKDSALVAAARLSHRYITDRFLPDKAI
                     DLVDEAASKLAMELESVPTEIDEVQRRLRQLELASRQLAEETEESAVERREEIEAEIV
                     ATKRKLASLREQWEAEKLGVGDVQKLRKDLDRATLEYGQLEAQIKEKQSAGIPPSEED
                     YQKLLKLYNQRQTLEKQIEASDETAEQKPADRRLLRREVTEDEIAEVVAAWTGIPVSR
                     MLETEKAKLLVMEERLHQRVVGQDEAVSAVSAAVRRSRAGLQDQHRPIGSFLFLGPTG
                     VGKTELCKALAEVMFDSDQAVVRIDMSEFGEKHNVSRLIGAPPGYIGYEEGGRLTEAV
                     RRRPYSVVLLDEIEKAHRDVFNVLLQVLDDGRLTDGQGRTVDFTNTIIV"
     gene            2922..>3904
                     /locus_tag="VFB80_18145"
     CDS             2922..>3904
                     /locus_tag="VFB80_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HZN35759.1"
                     /translation="MAGFVRGWGSASRGSAPHTGRRLGLEPLEARCLLAADFSDPFAE
                     LLTHGGACGCPICTGNGLAAIAPEAATATTAAAATFSLSSTPLSVPQLSSRPGAKATL
                     FLDFNGNVESRWGSETNAVTPAFDTDGNYSSYSAAELAAIREIWARVAEDYAPFNVNV
                     TTVAPPLLADRVAAKIVIGGNFSDWFGSSAGGVAYVGGFANGASNVGYVFEDALGNGN
                     PRFVAEAATHEAGHLFGLSHQASWSGGSLSSEYNSGNGTWAPIMGVGYYADRTTWHRG
                     PTTAGAASIQDDMSVLAGSANGFGFAADDFGSTTAAAAALPTSGGSVNVAGL"
BASE COUNT          596 a         1270 c         1287 g          751 t
ORIGIN      
        1 atcacgatga tcgtgttcgt gaaatcgacg gtgcggccct gaccgtcggt cagccggccg
       61 tcgtcgagca cctgcaacag cacgttgaat acgtcccggt gggccttttc gatttcgtcg
      121 agcaggacca cgctgtacgg ccggcggcga accgcctcgg tcaagcgccc gccctcttcg
      181 tagccgatat agcccggcgg agcgccgatc aggcggctga cgttgtgctt ctcgccgaac
      241 tcgctcatgt cgatgcggac cacggcctgg tcggagtcga acatcacctc ggccagggcc
      301 ttgcagagct cggtcttgcc cacgcccgtc ggacccaaaa acaggaacga gccgattggc
      361 cgatgctggt cctgcaggcc ggcccggctg cggcggaccg cggcgctgac cgccgacacc
      421 gcctcgtcct gcccgaccac ccgctggtgc agccgctcct ccatcaccag caacttggcc
      481 ttctcggttt cgagcatgcg gctgacgggg atgccggtcc aggcggcgac gacctcggcg
      541 atttcgtcct cggtcacctc gcggcgcaag aggcggcgat cggcgggctt ctgctcggcc
      601 gtctcgtcgg aggcctcgat ctgcttctcc agcgtctgcc gctggttgta gagcttgagc
      661 agcttctgat agtcctcttc gctcggcgga atgccggcgg actgcttctc cttgatctgg
      721 gcttcgagct ggccgtattc cagcgtcgcg cggtcgaggt ccttgcggag cttctgcacg
      781 tcgccgactc cgagcttctc ggcctcccac tgctcgcgga ggctggccag cttccgcttg
      841 gtggcgacga tttccgcttc gatctcttcg cgccgctcca cggccgattc ttcggtctcc
      901 tcggccagtt gccgcgaggc cagttccagc tgccgcagcc gccgctgcac ctcgtcgatc
      961 tcggtcggca cgctttcgag ttccattgcc agcttgctgg cggcctcatc gaccaggtcg
     1021 atcgccttgt cgggcaggaa gcggtcggtg atgtagcggt gcgagagccg ggcggcagcg
     1081 accagggcgc tgtccttgat cttcacgccg tggtgggcct cgtagcgggg cttcaggccg
     1141 cggaggatgc tgatcgtgtc ctcgacgttc ggctcgccga cgtagaccgg ctggaaccgc
     1201 cgctccaaag ccgcgtcctt ctcgacgtgc tggcggtatt cgtcgagcgt cgtcgcgccg
     1261 atgcaccgca attcgccgcg ggccagggcc ggcttgagga gctgcgccgc gtcggccgcc
     1321 ccttcagcct tccccgcgcc gaccacggtg tgcatctcgt cgatgaacag gatcacctgc
     1381 ccgttggact cctggacttc acggatgacg gccttcagcc gctcttcgaa ttgtccgcgg
     1441 tattgcgcgc cggccacgag caggcccatg tcgagggcga tgacgcggcg gttcttcagg
     1501 ctctgcggca cgtcgccctg cacgatccgc tgggccagcc cctcgacgat ggccgttttg
     1561 ccgactcccg gctcgccgat cagcacgggg ttgttcttcg tccgccgcga gaggacctgg
     1621 atcacgcggc ggatttcctg gtcgcgaccg atcaccggat cgagtttgcc ggcggtcgct
     1681 ttctcgacca ggtcgatgcc atattttttc agggcttgat acttgccttc cgggttctgg
     1741 tcggtgacgc gggccgagcc gcggaccgat ttcagcgctt cgagaacgtc cttctcgcgg
     1801 acgccgttga cttgcagcag gctcttggcg ggcgaatcga ccttcgtcag ggccagcagc
     1861 aagtgctcga ccgagacgaa ttcgtccttc atcgccgatg cttcctggag cgccccttcc
     1921 aggacagcca tcagctggcg gttgggattc ggctgcgtcc cgccgctgac ttgcggccgg
     1981 cggtcgagtt ccgcccgcac ctgccgcaac atttgctgca cgttgacgcc gaccttctcc
     2041 aggaccgggc gggcgacgcc ttcggcatcc tccagcatcg ccgccagcag gtgcagcggc
     2101 tccatctcgg cctggccctt atcggccgcc aggctctggg cgcgctgcac cgcctcttgc
     2161 gatttgacgg tcaatttgtc gaagcggaaa gccatagcga gccaggggtc aggagccagg
     2221 ggtcaggagt cggcgcaggc gaccgcattt ttggcggtgc ctggccctgg agaggggaaa
     2281 tggcaaacgc cattccaaat gccggcgaca ggctcccgcg agcaacatgc gcgcataccg
     2341 cacgattttt cctgagacgc aattctgatc gcaaatgctt gtgttgaaac aacttgcgtg
     2401 cgaaatccag cgccggcgat tatgcgcatt atgcgcgctg gcggtacttg gggaaccagc
     2461 gatggctggc cgagagttgg agatgcaata tgcatgaatc agaattgttg ttccgcgagg
     2521 gattcgactt taactgctta tgcgatacgg gcttacgact atcctgcggc atgcatcgta
     2581 tgcattttat gcaggttaag gtggcagtat gctcgtttcg ccggatactc gccgccggca
     2641 cgaaagttca cagtatttta gctatgtgta cacgtgtggc aagcagccca aagaaggagg
     2701 cccaatcgag caggcttgct cgggccatcc atcagccgcg aaacatgcct caccgcaggc
     2761 gtagcagccc gctcgccagc ggcggttgct gccgcggaaa tcgagcagga ttgccgttgg
     2821 tgacgggccg ataggttcct acagtggccc gcttggttcg agcgcggctg gatgctgcgc
     2881 gtctgtcggg tgctagttcc acttgctgca aggagtgcgg catggctgga ttcgttcggg
     2941 gttggggcag tgcgtcaaga ggcagtgcac cacacaccgg acgaagactc ggcctcgagc
     3001 cgctggaggc ccgctgcctg ctggcagctg atttcagcga cccgtttgcg gagctgctga
     3061 cgcatggcgg ggcctgcggc tgcccgatct gcactggcaa tggcctggcg gcgattgcac
     3121 cggaggctgc gaccgccacg acggccgcag cagccacttt ttcgctaagc agcactcccc
     3181 tgagcgtgcc gcagctcagc agccggccgg gggcgaaggc tacgctgttt ctggatttca
     3241 acggcaacgt ggagtcgcgg tggggctcgg agacgaatgc ggtcacgccg gcctttgata
     3301 cggacggcaa ctacagcagc tatagcgcgg cggagctggc ggcgatccgc gagatttggg
     3361 cccgcgtggc ggaagactac gccccattca acgtcaacgt gacgaccgtg gccccgccgc
     3421 tgctcgccga ccgcgtggcg gcgaagatcg tcatcggcgg gaatttctcc gactggttcg
     3481 gcagctcggc cggcggcgtg gcctatgtcg gcggctttgc gaacggggcc tcgaacgtcg
     3541 gctacgtgtt cgaagacgcc ctggggaacg gcaacccgcg gttcgtggcc gaggcggcga
     3601 cccacgaagc cgggcacctg ttcggccttt cacaccaggc cagctggagc ggcgggtcgc
     3661 tgagctcgga atacaactcg ggcaacggca cttgggcgcc aatcatgggc gtcgggtatt
     3721 acgccgaccg cacgacctgg caccgcgggc cgaccacggc cggcgcggcg tcgatccagg
     3781 acgacatgtc cgtgctggcc ggctcggcca acggattcgg cttcgcggcc gacgatttcg
     3841 gcagcacgac cgcggcggcg gcggctttgc ccaccagcgg cgggtcggtg aacgtggcgg
     3901 gact
//
LOCUS       DASRJB010000683         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2165395, whole genome shotgun sequence.
ACCESSION   DASRJB010000683 DASRJB010000000
VERSION     DASRJB010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165395"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFB80_18150"
     CDS             <1..1257
                     /locus_tag="VFB80_18150"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN35760.1"
                     /translation="GTPEQVAALNARNEELGKLDAKVLEIITKKPADAKEQQAAIRER
                     QQELRAQTPDRPGAYRCFEDSAEAPPTYLLLSGRASNPGPLMQPRVPAVLVGNALRGV
                     PAQPQFPLPTAKSTLRRLTLARWNASDANPLTARVIVNRVWQHHFGTGLVATPSDFGQ
                     MGARPTHPELLDWLAHWFTHDANWSLKKLHRLIVTSETYRQANSSPHAPREAGISRSE
                     MTTMADPENRLLWHFPYRRLDVEAIRDSMLAAAGNLDRRMYGPAVKLPIPAAAIEAHT
                     DKQGAWKAAGEPDINRRTVYAFVKRTLLVPMLETLDFCDTTSSTDRRAITSIAPQALT
                     LFNGDFTSRQAEVFAQRLEREAGEDPQRQIDLAYRAALARPPTAEESAAMTAFLQREA
                     SVTDQHAALVQACRVLLNLNEFVYPN"
     gene            complement(1340..1585)
                     /locus_tag="VFB80_18155"
     CDS             complement(1340..1585)
                     /locus_tag="VFB80_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35761.1"
                     /translation="MRYKLRTLLILLAIMPPLLWIGWTKYAAWKAEQGNQRVVLLIDV
                     VETISVNDLGAPDARIIVQPLPADAPPAIVPEEPESR"
     gene            complement(1600..1842)
                     /locus_tag="VFB80_18160"
     CDS             complement(1600..1842)
                     /locus_tag="VFB80_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35762.1"
                     /translation="MFRFTIRDVLWLTVVVALAVGWWVERQAARKTAAKNEELLARVA
                     HLTERAENAEHWVNKWRPIVSGSSGLLSESGKPNKP"
BASE COUNT          388 a          737 c          601 g          320 t
ORIGIN      
        1 ggcacgcccg agcaggtcgc cgcgctcaat gcccgcaacg aagagctggg caagctcgac
       61 gccaaggtcc tggaaatcat caccaaaaaa cctgccgatg ccaaagagca gcaggcagcg
      121 atccgcgagc ggcagcagga attgcgggcc cagacgccgg acaggcccgg cgcttaccgc
      181 tgcttcgaag acagcgccga agctccgccc acgtatctgc tgctctccgg ccgcgccagc
      241 aatcccggcc cgctgatgca gccgcgagtt cccgcggtgc ttgtagggaa cgccctccgc
      301 ggcgttccgg ctcagccgca gtttccgctt cccaccgcga aatccacgct ccgtcggctg
      361 acgctggccc gctggaacgc cagcgacgcc aacccgctga cggcgcgggt catcgtcaac
      421 cgcgtctggc agcaccattt tggcacgggg ctggtcgcca cgcccagcga cttcggccag
      481 atgggagccc ggccgacgca cccggagctg ctcgactggc tggcccactg gttcacgcac
      541 gacgccaatt ggtcactgaa aaagctgcac cggctgatcg tcacgagcga aacgtaccgg
      601 caggccaata gtagtcctca cgctccgcgt gaggccggca tctcgcggag cgagatgact
      661 actatggccg accccgaaaa ccgcctcctc tggcacttcc cctaccgccg gctggacgtc
      721 gaggcgattc gcgattcgat gctggccgcg gcggggaacc tggatcggcg gatgtatggc
      781 ccggcggtca agctgccgat tcccgcggcg gcgatcgagg cccacaccga caagcagggg
      841 gcgtggaagg cggcgggcga gcccgacatc aaccgccgca cggtgtatgc cttcgtcaag
      901 cgaacgctgc tcgtgccgat gctcgagacg ctcgacttct gcgacacgac cagcagcacc
      961 gaccggcggg cgatcaccag catcgccccc caggccctga cgctcttcaa cggcgacttc
     1021 accagccggc aggcggaagt cttcgcccag cgcttggagc gcgaagccgg cgaagatccc
     1081 cagcggcaga tcgacctcgc ctatcgcgcc gccctcgccc gtccgccgac ggccgaggag
     1141 tcggccgcga tgacggcgtt cctccagcgc gaagccagtg tcaccgacca gcacgccgcc
     1201 ctcgtccagg cctgccgcgt gctcctcaat ctcaacgagt tcgtgtatcc gaattagccg
     1261 atcgatccgg gtggcactgg cgtttcgcca gtgctcctcc ggctttctgt gtaaacgtcg
     1321 tgtcagcggc ggagtttccc taccgcgact ccggctcctc cggaacgatg gccggcggcg
     1381 cgtctgccgg cagtggctgg acgatgattc gcgcgtcagg cgcacccaga tcgttgacgc
     1441 ttatcgtttc caccacgtca atcaggagca ccacgcgctg attcccttgc tccgccttcc
     1501 acgccgcgta cttcgtccag ccgatccaca gcagcggcgg catgatcgcc agcaggatca
     1561 gcagcgtgcg gagtttgtag cgcatcaata agcaaccggt cacggtttat tgggcttgcc
     1621 gctctccgac aataagcccg acgaacctga gacaattggc cgccacttat tgacccagtg
     1681 ttcagcattc tcagcgcgct ccgtcaaatg agcaacgcgc gcgagcaact cctcgttctt
     1741 tgccgccgtc ttgcgtgccg cctgcctctc cacccaccag cccaccgcca gcgccaccac
     1801 caccgtcagc cacagcacgt cgcggatcgt gaatcggaac atggacaacc tcagtaatgt
     1861 cgttgcgtcg cgatgcccga gatgatccag aatccgaggc agaccgctac gattgaacaa
     1921 caaacaatcc acgctgcgag aaggtcgtgg tccgcgcgac tcaacgcgtt cgcataagac
     1981 gacactcccg cagcaccagc ggcccaaccg ccaggacgag catcagagtc cgaaggctgt
     2041 agcgca
//
LOCUS       DASRJB010000684        11328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1324481, whole genome shotgun sequence.
ACCESSION   DASRJB010000684 DASRJB010000000
VERSION     DASRJB010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324481"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFB80_18165"
     CDS             complement(<1..280)
                     /locus_tag="VFB80_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35763.1"
                     /translation="MLASWTKLLTISIAAALVGCKSGTPPPEVPPWLAMQPLAAPTPA
                     AALLTPQFGLVRLPPVDPEPPPEIATASWNAPVQGSEVVFHSATITEQP"
     gene            complement(398..3469)
                     /locus_tag="VFB80_18170"
     CDS             complement(398..3469)
                     /locus_tag="VFB80_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN35764.1"
                     /translation="MTWLVSTSLKLRVIVLALACLLVAFGIRTLRTTPLDVFPEFAPP
                     LVEIQTEAPGLSTEEVESMVTMPLENALSGTPWKKIIRSKSVLGLSSVVLIFEDGTDL
                     IRARQLVQERLATEASRLPAVAHPPVILSPLSSTSRALKIGITSKTLSQMEMTELARW
                     TIRPRLMSVPGVANVAIWGQRDRQFQVLVDPQRLRTHDVPLATVERAAADAVALDGGG
                     FVDTPNQRLAVRQQVGIYDAEDLGETMVAFRSGTPLRLRDVADVVESFPPPIGDAVIN
                     DVPGLLLIVEKQPWGNTLEVTRGVEAALTALAPGLKELEIDPTIFRPATYIERSLYNL
                     SHAMAVGCILVVVILVVFLFDWRTALISLTAIPLSLIAAGLVLHYWGGTLNTMVIAGL
                     VIALGEVVDDAIIDVENILRRLKLNADSPTRQTPFQVVLAASLEVRSAVVYASLIVTL
                     VFVPVLFLGGLAGSFFRPLAIAYILAILASLVVALTVTPALSLLLLPSAGYERAEAPL
                     ARWLKAIYRGLLPPLVARPGMSLIIVIVSLGLSVFAFFQLGRELLPNFQETDFLMHWV
                     EKPGASLDSMQRITTQASRELRAIPGVRNFGSHIGRAEVADEVVGPNFTELWISVDPS
                     VDLPATVAKVQAAVDGYPGLYRDLLTYLKERIKEVLSGAGASIVVRVYGPDLAVLRAK
                     GEEIKHVMEGVAGVTNLKVEAQVLVPQVQVVLRPDAASRLGLSPGQVRHAVATLVKGT
                     KVGEVYQDQKTFDVVVRGTPEVRTDLAALRELVLDLPLGGQAPLKDVADVFIEPAPNE
                     IKREAASRRLDITCNVKDRDLGSVAREIEQQVSALPFDREYHPEFLGEYAALRESTIR
                     LFVLFLLAAVGILLLLHMDFQSWRLTFLVAFTIPFALVGGVLGTILQGGILSLGSLVG
                     FVTVLGIAARNGIMLVSHYRHLEEVEGEPFGVGLVLRGAEERLTPILMTALATGLALV
                     PLVVSGNKPGHEIEYPMAVVILGGLTTSTLLNLFLLPPIYALFGRVQRTAE"
     gene            complement(3479..4729)
                     /locus_tag="VFB80_18175"
     CDS             complement(3479..4729)
                     /locus_tag="VFB80_18175"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN35765.1"
                     /translation="MIALAGLFAWGCQQPANKPAPVSPAKVAAVAQEGKLNTLELTED
                     AEKRLGITLATVVEREVHRVRTYSGEIALPPGASLVISAPVGGKLQAPGSATVPEVGA
                     LVAASQPIFVLTPLLSPERDVLTPAERIALAQAKNAIATSRIDAAGQVEQARVQVEAA
                     KIALERAERLYRESAGTARAVDDAKAQHGIATKGLEAAQSRLKLLDEINLEGGAGGEQ
                     TPLVLEAPQGGLIRTRSAAPGEVVAAGAPLFEVMRFNPIWVRVPVYAGETSDLALDQP
                     AQVAPLNAGDHVAALEARPIAAPPTATPLSATVDLYYELPNPDAKLRPGERVTVRVKL
                     PASSGPQRVIPWSAVIHDISGGTWVYEQTAERTYVRRRVQVRYVVEEEGQQLAVLAGG
                     PELGAKVATAAVVELFGTEFGFAK"
     gene            complement(4831..6882)
                     /gene="tkt"
                     /locus_tag="VFB80_18180"
     CDS             complement(4831..6882)
                     /gene="tkt"
                     /locus_tag="VFB80_18180"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZN35766.1"
                     /translation="MSTPSIEQLAINTIRTLSMDAVQKANSGHPGTPMALAPVAYELW
                     QRHLRYDPSEPLWPGRDRFVLSCGHASMLLYSVLHLAGVRRVDHHGNPQDGLAITEGD
                     IKNFRQLGSPCAGHPEYGEAAGIETTTGPLGQGVGNSVGMALAARWLAAHYNRPGFEL
                     FDFRTYALCSDGDLMEGISGEAASLAGHLKLGNLLWIYDDNSITIEGETELAFSEDVA
                     KRFAGYGWNTLRVDDANDLAAIGRAIEAAKQTTDRPTIIILKSIIGYGAPNKANTHAA
                     HGAPLGADEVKLTKKFYGWPEDAQFLVPDEVRKHFAEGVGRRGQAAREAWERKLAEYA
                     KSFPKEAAELRTMWAGKLPEGWDKGIPTFPADAKGLASRVSSGKVLNGLAKHYPWLIG
                     GSADLAPSTMTLLEHTDSLSSHNYGGRNLHFGIREHGMAAVCNGLSLCGIRPYGATFF
                     VFTDYMRPALRLSAIMRRPVIYVLTHDSIGLGEDGPTHQPIEHLSACRAIPQLDVIRP
                     GDANEVAECYRTAIQRMHHPAAMVLSRQNLPTLDRTKFAPASLCGRGAYVLAEAPGGK
                     PQAIVMATGSELDIAVKAWEKLTAEGIAVRLVSVPCFEWFDEQDDAYRASVLPREIKA
                     RVAVEAGVRQCWDRFLGEHGRFVGMSTFGGSAPYAALYKHFGITPEHVIAEVKAALGR
                     A"
     gene            complement(6970..7461)
                     /gene="smpB"
                     /locus_tag="VFB80_18185"
     CDS             complement(6970..7461)
                     /gene="smpB"
                     /locus_tag="VFB80_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZN35767.1"
                     /translation="MAKVEKEKTPVEPEVRLVSENRKARFRFEILDTLECGIQLTGSE
                     VKSLRDGKLSLDEAYARVKEGEVWLVGADIAEYPQATLWNHLPKRPRKLLMKRSEVSK
                     FAGKAHEKGLTLVPLKVYFNARGLAKVLLGLGKGKKLHDKRESLKKADSQREMDSAMR
                     RST"
     gene            7635..8333
                     /locus_tag="VFB80_18190"
     CDS             7635..8333
                     /locus_tag="VFB80_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35768.1"
                     /translation="MHIHLLGTVDYEDCLTLQRRLAYDAMTRADGRIVALICEHPPLV
                     TIGRAGSRAHVRFTGAELAQRQLTIRYVGRGGGAILHAPGQLAVYPIVPLGSRGWTIG
                     DYLRRLQQALARVLVELQVRPLAVPGSFALAGRSGILAVIGAAVRQGVTQHGAYLNVN
                     PAMRDFGRVEVAAGQTMSSLLSQLALPTKMTRVRAALVMHLAEALELERYHLHTGHPL
                     LAELPAASTRESAA"
     gene            8362..9312
                     /gene="lipA"
                     /locus_tag="VFB80_18195"
     CDS             8362..9312
                     /gene="lipA"
                     /locus_tag="VFB80_18195"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZN35769.1"
                     /translation="MVAGAGETTSFGACDSGTATNGRGFDRLPRWLKRNVPKGNANHF
                     TAGLLEELGLETVCDNAKCPNRMECYSQKTATFMILGNVCTRPCGFCAVERGRPAPPA
                     ADEPDRLAEAAARLALAHVVITSVTRDDLPDGGAEHFHRCVLAVRRRTGAAVEVLTPD
                     FVHNLAALDRVIDAAPEVFNHNTETVPRLYRQVRGPKSDFRWTLALLRRVKDRNPAIK
                     TKSGLMLGLGETREELFDTLAALRDAGCELLTLGQYLMPSSALADHYLPVVRYLPPEE
                     FAELGRAARALGFAQVASGPFVRSSYHARELAEPADASGA"
     gene            complement(9649..10461)
                     /locus_tag="VFB80_18200"
     CDS             complement(9649..10461)
                     /locus_tag="VFB80_18200"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HZN35770.1"
                     /translation="MPIVFHLKRPPTVPLEAEVLSPDVVAGLSNAEIRTLTVYHGKRQ
                     LPLREFFDVEGEKSSDLILHGALHKVRWVGRAMSQGSITIHGSVGMHLGAYMSGGRIE
                     VHGNATDWIGAEMKNGFIHIHGSAGGQVGAAYRGSLRGMRGGTIIVDGSAGLEVGMRM
                     RRGTIVIRGPARDFTGLQMKGGTIVLESGAEIRTGAWMNRGTIISLKPLQLMPTFAFA
                     AKVHPLFIRLLDNHLRPFGVTLPGPADGAYRHYSGDLAVSDKGEVLVWEARA"
     gene            complement(10688..>11328)
                     /locus_tag="VFB80_18205"
     CDS             complement(10688..>11328)
                     /locus_tag="VFB80_18205"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN35771.1"
                     /translation="TGCGIVPYTYRPSNLVNAVQWAAGLELLLLIKSPWQVFLSTDHP
                     NGGCFWRYPEIIELLMRRDVRKEQLKRLPDKIKGRITLPEIDREYTLLEIATAMSAGP
                     ARALGLAQKGNLSVGSDADLVLYHEDENIAQMFSHPRYVIKGGEIVLEDGEIREAPQG
                     REFLVRPAMDPATNEFMRPLFEDCYTMSFENYPVEIERIEHRELRECVPVGK"
BASE COUNT         1870 a         3775 c         3761 g         1922 t
ORIGIN      
        1 gcggctgctc cgtgatagtc gccgagtgaa acacaacctc cgaaccctgg acgggcgcat
       61 tccagcttgc ggtcgcgatc tccggcggag gctcgggatc gaccggcgga agacgcacga
      121 gcccgaattg cggcgtcagg agtgcggcag cgggtgttgg cgcggccaga ggctgcattg
      181 ccagccaagg cggcacttcc ggaggcggag taccgctctt gcagccgacg agcgcggccg
      241 cgatcgagat cgtcaggagt ttggtccaag atgcaagcat cgctagcagc tatcgcgacg
      301 gcgaccctgc cgtgcgcgca gagtctgacg aagattcctt cggacgccgt aaagcagagg
      361 gataaggcgc gcgcggcgaa ttgcagccgc tagcggccta ttccgcggtc cgctggacgc
      421 gtccgaacag ggcgtaaatc ggcggcagca ggaacaggtt cagcagcgtc gaggtcgtca
      481 gtccgccgag aatgaccacg gccatcgggt actcgatctc gtgccccggc ttgttgccgc
      541 tgacgaccag cggcacgaga gccagcccgg tggccagcgc ggtcatcagg atcggcgtca
      601 gccgctcttc cgcgccgcgg aggacgaggc ccacgccgaa gggctcgccc tcgacttcct
      661 ccaggtgccg gtagtggctg acgagcatga tgccgttgcg ggcggcgatg ccgagcacgg
      721 tgacgaagcc gacgagcgag ccgagcgaga ggatgccgcc ttgcaggatc gtccccagca
      781 cgccgccgac cagggcgaac gggatcgtga aggctacgag gaacgtcagc cgccaggact
      841 gaaagtccat atgcaagagc agcagaattc ccacggccgc cagcaggaag agcacgaaca
      901 ggcggatggt cgattcgcgg agcgcggcgt attcgcccag gaattccggg tgatactcgc
      961 ggtcgaacgg cagcgcgctg acttgctgct cgatttcgcg ggcgacggag cccaggtcgc
     1021 ggtccttgac gttgcaggtg atgtcaagcc ggcgcgaagc cgcctcgcgc ttgatctcgt
     1081 tgggggccgg ctcgataaaa acgtcggcga cgtccttgag cggggcttgc ccgcccagcg
     1141 gcaagtcgag gaccagctcg cggagagccg ccaggtcggt gcggacttca ggcgtgcccc
     1201 gtacgacgac gtcgaacgtc ttctggtcct ggtagacctc gccgaccttc gtccctttga
     1261 ccagcgtcgc caccgcatgc cgcacctgtc ccggcgacag gcccagccgc gacgcggcgt
     1321 cgggccgcag cacaacctgc acttgcggca cgagcacctg ggcctcgacc ttgagattgg
     1381 tcacgccggc gactccttcc atgacgtgct tgatctcctc gcccttggcc cgcaacacag
     1441 cgaggtcggg gccgtaaacc cgcacgacga ttgacgcccc cgctccggag agcacttcct
     1501 tgatccgctc cttcaaatac gtgagtaagt cgcgatacag gcccggatag ccatcgacgg
     1561 cggcctgcac cttggcgacc gtcgctggca gatcgaccga cggatcgacg ctgatccaca
     1621 gctcggtgaa gttcggcccg acgacctcgt cggcgacttc cgcccggccg atgtgcgagc
     1681 caaagttccg cacgcccggg atcgcccgca gctcgcggct ggcctgcgtg gtgatccgct
     1741 gcatcgaatc gagcgaggcg cccggcttct cgacccagtg catcaggaag tcggtctcct
     1801 gaaagttcgg cagcagctcc cgcccgagct gaaagaaggc aaagaccgaa agcccaagcg
     1861 agacgatcac gatgatcagc gacatcccgg gccgcgcgac gagcggcggg agcaggccgc
     1921 gatagatggc cttcagccag cgggccagcg gcgcttcggc ccgctcgtaa ccggccgatg
     1981 gcagcagcaa gagcgacagc gccggcgtga ccgtcaatgc gaccacgagc gaggccagga
     2041 ttgccaggat gtaggcgatc gccagcggcc ggaagaagga gccggccagg ccgcccagga
     2101 acagaaccgg cacgaagacc agcgtcacga tcaggctggc ataaacgacg gcgctgcgga
     2161 cttcgagcga cgccgccaac acgacctgaa acggggtttg cctggtgggg ctgtcggcgt
     2221 tcagcttcag ccgccgcagg atgttctcga cgtcgatgat cgcgtcgtcc acgacctcgc
     2281 ccagcgcgat caccaggccc gcgatgacca tcgtattgag cgtgccgccc caatagtgca
     2341 gcaccaggcc ggcggcgatc agcgacagcg gaatggccgt caggctgatc agcgccgttc
     2401 gccaatcgaa caggaacacg actagaatga cgacgaccag gatgcagccg acggccatcg
     2461 cgtgcgacag gttgtagagc gagcgttcga tgtaggtcgc ggggcgaaag atcgtggggt
     2521 cgatctccag ctctttgagt ccgggtgcga gcgcggtcaa ggccgcttcg acgccgcgcg
     2581 tcacctccag cgtgttgccc cacggctgtt tttccacgat cagcaatagg cccggcacgt
     2641 cgttgatgac tgcgtcgcca atcggcggag ggaagctctc gaccacgtcg gccacgtccc
     2701 gcagccgcag gggagtgccg ctgcggaagg cgaccatcgt ctcgcccaag tcctcggcgt
     2761 cgtagatgcc gacctgctgg cggacggcca gccgttggtt cggcgtatcg acgaagccgc
     2821 cgccgtcgag cgcgaccgcg tcggccgccg cccgctcgac ggtcgccagc ggcacgtcgt
     2881 gcgtgcgcag ccgctgcgga tcgacgagca cttgaaattg ccggtcacgc tgcccccaga
     2941 tcgcgacgtt ggcgacgccg ggcaccgaca tcagccgcgg gcgaatcgtc cagcgagcca
     3001 gctccgtcat ctccatctgc gacagcgtct tcgacgtgat gccgatcttg agggcccggc
     3061 tggtcgagga gaggggcgag aggatcacgg gcggatgggc gacggcgggc aggcggctgg
     3121 cttcggtcgc caggcgctcc tgcacgagct ggcgggcacg gatcaagtcg gtgccgtcct
     3181 cgaagatgag cacaaccgac gacaggccca gcaccgactt gctgcgaatg attttcttcc
     3241 agggcgtgcc actcaaggcg ttttccaacg gcatcgtgac catgctctcg acctcttcgg
     3301 tcgagagacc cggcgcttcg gtctggattt ccacgagcgg cggggcgaac tccgggaaca
     3361 cgtcgagcgg cgtcgtccgc agcgtgcgga tgccgaaagc gaccagcagg caggccagcg
     3421 ccaggacgat gacgcgcagc ttcagcgagg tggaaacgag ccaggtcata ctcaacggct
     3481 atttcgcaaa gccgaattcc gtgccaaaga gttcaaccac ggcggccgtg gcgactttcg
     3541 cgccgagttc cgggccgccg gccaatacgg cgagctgctg gccttcttcc tcgacgacgt
     3601 agcgcacctg cacccggcga cggacatagg tccgctcggc cgtctgctcg tagacccaag
     3661 tcccgccgct gatgtcgtga atcacggccg accagggaat gacccgctgc ggaccgctgc
     3721 tggcgggcag cttgacgcgg accgtcaccc gctcgccggg acgcagcttc gcgtcgggat
     3781 tgggcagttc gtagtacaga tcgacggtcg ccgaaagggg cgttgccgtc ggcggcgcgg
     3841 cgatcggtcg ggcctccagc gccgcaacgt gatcgccggc attgagcggc gcgacttgtg
     3901 ccggctggtc gagcgccaag tcgctggttt cgccggcata gaccggcacg cggacccaaa
     3961 tcggattgaa ccgcatcact tcgaagagcg gcgcgcctgc ggcgaccacc tcgcccggcg
     4021 ccgccgagcg cgtgcgaatc aggccgccct gcggggcttc gagcaccagc ggcgtctgct
     4081 ctcctccggc gcccccttcc aggttgatct cgtcgagtag tttcagccgc gattgggccg
     4141 cttccagtcc tttcgtcgcg atgccgtgct gggccttggc gtcatcgacg gcccgcgcgg
     4201 tgccggccga ttcgcgatac agccgctcgg cccgctccag ggcgattttg gccgcttcga
     4261 cctgcacacg ggcttgctcg acctggccgg cggcgtcgat ccgcgaagtg gcgatcgcgt
     4321 tcttggcctg ggccagggcg atccgttcgg ccggagtcag tacgtcgcgc tccggcgaaa
     4381 gcagcggcgt cagcacgaag atcggctggc tggcggcgac gagcgctcca acctccggca
     4441 cggttgccga tccgggcgct tgcagcttgc cgccgaccgg cgcgctaatg accagcgacg
     4501 cgcccggcgg cagggcaatc tcgccgctgt aggtccgcac gcggtggact tctctctcaa
     4561 caacggtcgc gagcgtgatt ccgagacgct tctctgcatc ctcggtcagc tccagtgtgt
     4621 tgagcttccc ctcctgcgcg actgcggcaa ctttggcagg cgatacgggc gcgggcttat
     4681 tcgcgggctg ttggcagccc caggcaaaaa gcccggcgag ggcgatcacg gctcgttggg
     4741 gttgagatgg acgcatggag tgtcgcggct cggctccaga ttagttatcc cgccgagaaa
     4801 agaccacacg aacgtttgcc gccggtagtc tcaggccctg ccgagtgccg ctttcacttc
     4861 ggcgatcacg tgctcgggcg tgatgccaaa atgcttgtag agcgcggcgt aaggggcgct
     4921 gccgccgaag gtgctcatgc cgacgaagcg gccgtgctcg cccaggaatc ggtcccagca
     4981 ttgccgcacg ccggcttcca cggcgacgcg ggccttgatc tcgcgcggca atacgctggc
     5041 gcggtaggcg tcgtcctgct cgtcgaacca ctcgaaacac ggcacgctga ccaggcgcac
     5101 ggcaatgcct tcggccgtga gcttctccca ggccttgacg gcgatgtcga gctcgctgcc
     5161 cgtggccatg acgatcgcct gcggcttacc gcccggcgct tcggccagga cgtacgcccc
     5221 gcggccacag agcgaagccg gcgcgaactt cgtgcggtcg agcgtaggga ggttttgccg
     5281 cgacagcacc attgctgccg gatggtgcat ccgctggatc gctgtgcggt agcattcggc
     5341 cacttcattg gcgtcgccgg ggcggatcac gtcgagctgc gggatcgccc ggcacgccga
     5401 caggtgctcg atcggctgat gcgtcgggcc gtcctcgccc aggccgatcg agtcgtgcgt
     5461 caggacgtag atcaccggcc gccgcatgat ggccgacagc cgcagcgccg gccgcatata
     5521 gtcggtgaag acgaaaaacg tcgcgccgta ggggcggatg ccgcacagcg agaggccgtt
     5581 gcagaccgcg gccatgccgt gctcgcggat gccgaagtgg aggttgcggc cgccgtagtt
     5641 atggctgctc aggctgtcgg tgtgctccag cagcgtcatc gtcgaagggg cgaggtccgc
     5701 ggagccgccg atcagccacg ggtagtgctt cgccaggccg ttgagcacct tgcccgacga
     5761 gacgcggctg gcgaggcctt ttgcatcggc cgggaaagtc gggatgccct tgtcccagcc
     5821 ctcaggcagc ttgccggccc acatcgtccg cagctcggcg gcctccttgg gaaatgactt
     5881 ggcatactcg gccagcttcc gctcccaggc ttcgcgggcc gcctggccgc gtcggccgac
     5941 gccctcggca aagtgctttc gcacttcgtc gggcacgagg aactgggcgt cctcgggcca
     6001 gccgtagaac ttcttggtca gcttgacttc atcggccccg agcggagcgc cgtgggcggc
     6061 gtgagtgttg gccttattag gcgcgccgta gccgatgatg ctcttgagga taatgatcgt
     6121 cggccggtcg gtcgtttgct tcgccgcctc gatcgcccgc ccgatggccg ccaggtcgtt
     6181 ggcgtcatcg acgcggagcg tgttccagcc gtagccggcg aaccgcttgg caacatcttc
     6241 gctgaaggcc agctcggttt cgccttcgat cgtgatgctg ttgtcgtcgt agatccagag
     6301 caggttgccg agcttgaggt ggcccgcgag tgaggcggct tcgcccgaga tgccctccat
     6361 caggtcgccg tcgctgcaca gcgcataggt ccggaaatcg aagagctcga agcccggccg
     6421 gttgtagtgg gcggcgagcc acctggcggc cagggccatg cccacgctgt tgccgacgcc
     6481 ctggcccagc ggcccggtgg tcgtttcgat gcctgccgcc tcgccgtatt ccggatggcc
     6541 cgcgcagggg ctgccgagct gccggaagtt cttgatgtcg ccctccgtaa tcgccagccc
     6601 gtcttgcggg ttgccgtggt gatcgaccct ccgcacgccc gccaggtgga gcaccgaata
     6661 gagcagcatc gaggcgtggc cgcaggagag cacgaagcgg tcccgccccg gccagagcgg
     6721 ctcggacggg tcgtaccgca ggtgccgctg ccacagctcg tacgcgaccg gcgccagggc
     6781 catgggtgtg cccggatggc cgctgttggc cttctgcacg gcgtccatgc tgagcgtgcg
     6841 gatggtgttg atggcgagtt gttcgatcga aggcgtagac atgatctgca gattcgattt
     6901 cggtgacgta gattttgttg gccggtcagt ctagcccgcg ctccgcaaaa gcgtcaacga
     6961 cgcgacaggt cacgtgctgc gccgcatcgc gctgtccatt tcgcgctggc tgtcggcctt
     7021 tttcagcgac tcgcgcttgt cgtgcagctt cttgcccttg cccaagccga gcagcacctt
     7081 ggccaggccg cgggcgttga aatagacctt cagcggcacc agcgtcagcc ccttctcgtg
     7141 ggccttgccg gcgaacttgc tcacctcgga gcgcttcatg agcagcttcc gcgggcgctt
     7201 gggcaagtgg ttccagagcg tcgcctgcgg atactcggcg atgtccgctc cgaccagcca
     7261 gacctcgccc tccttcacgc gggcataggc ctcgtcgagc gacagcttgc cgtcgcggag
     7321 actcttcacc tcgctgcccg tgagctgaat cccgcactcc agcgtgtcga gaatctcgaa
     7381 ccggaaccgc gccttgcgat tctccgaaac cagccgcacc tctggttcaa cgggagtttt
     7441 ctctttttcg accttggcca tgcctctttt gtaagggaag cactcggcaa gcgggaggga
     7501 gcgcgatctg cccttggccc gcctgcggcc gcctgctaca gtccccgccc ctcgccggct
     7561 tctaccctcg tgattgcggc gaaatggcaa tggagaggac gctagcgcca caacccctcg
     7621 cgcgcgactg cccagtgcac atccacctgc tgggtacggt cgattacgag gactgcctca
     7681 cactccagcg gcggctggcc tatgacgcga tgacgcgggc cgacggccgc atcgtggcgc
     7741 taatctgcga acatccgccg ctcgtcacca tcggccgggc cggctcgcgg gcccacgtcc
     7801 gcttcaccgg ggccgagttg gcccagcggc aactgaccat tcgctacgtc ggtcgcggcg
     7861 gcggggcgat cctgcacgcc ccgggccagc tggccgtcta tccgatcgtg ccgctgggca
     7921 gccgcggctg gacgatcggc gactacctcc gccgcttgca gcaggccctg gcccgtgtgc
     7981 tggtcgagct gcaagtccgc ccgctggccg tgccgggcag cttcgccctg gccggccgca
     8041 gcggaattct ggccgtcatc ggtgcggccg tgcggcaggg cgtgacccag cacggcgctt
     8101 atctgaacgt caatccggcg atgcgcgact tcggccgcgt ggaggtcgcc gccggacaaa
     8161 ccatgtcgtc actcctgtcg cagctcgctt tgcccactaa aatgacgcgg gtacgggccg
     8221 cgctcgtcat gcacctggcc gaagccttgg agctggagcg ctatcacctg cataccggcc
     8281 atccgctcct ggcggaactg ccggccgcga gcacgcgtga atccgcagct tgaattgccg
     8341 ctgtttgcaa agccgcgacc attggtcgcg ggagccggcg aaacaacctc ctttggcgca
     8401 tgcgactcgg gcaccgcgac caatggtcgc ggctttgatc gcctgccccg ctggctgaag
     8461 cggaacgtgc ccaaaggcaa cgccaaccac ttcacggcgg ggctcctgga ggaattgggg
     8521 ctggaaacgg tttgcgacaa cgccaaatgt ccgaaccgga tggagtgcta ttcgcagaag
     8581 accgcgacat tcatgatcct gggcaacgtc tgcacgcggc cctgcggctt ctgcgccgtc
     8641 gagcgcgggc ggcccgcgcc gcccgcagct gacgagcccg atcggctggc cgaggcggcg
     8701 gctcggctgg ccctggcgca tgtggtgatc acgtccgtca cccgcgacga tctgcccgat
     8761 ggcggcgcgg agcatttcca ccgctgcgtg ctggccgtgc gccggcggac gggtgcagct
     8821 gtcgaggtgc tcacgcctga cttcgtccac aacctggcgg cgctcgaccg cgtgatcgac
     8881 gccgcgccgg aagtcttcaa ccacaacacg gagaccgtcc cgcggctgta ccggcaggtc
     8941 cgcggtccca agagcgactt ccgctggacg ctggccctgc ttcgccgggt gaaggaccgg
     9001 aacccggcga tcaaaaccaa aagcggactg atgctcggct tgggcgagac gcgcgaagaa
     9061 ctgttcgaca cgctggccgc cctccgcgac gccggctgcg agctgctgac gctcggtcaa
     9121 tacctcatgc cttccagcgc gctggccgac cactatctgc cggtcgtccg gtatctcccc
     9181 cccgaagagt tcgcggaatt gggccgtgcc gcccgcgcgc tcggctttgc acaggtcgcc
     9241 agcggcccct tcgtccgcag cagctaccac gcccgcgagc tggcggagcc cgcagacgcc
     9301 agtggcgctt agcggccgca agcattcgcc ggtcgatata agggaaaccc acattagctt
     9361 tccgcgctga cgatgacttt ggtgttgcac tttcccagct aacccattgg cttccaagca
     9421 gttgcgaccc cgactcgtcg tcgatgcggc agttggcttt gaagcattag agaagccgca
     9481 ggggagtttc ccttatattt cgcggccgat acgagtggca gcgatttttc cctagacaga
     9541 atttttcggc cgacgatggg ctggccaaaa tggcagtctt cgcgccaaat agaactgtag
     9601 agcaaatagc gtaccaatgt tcactaatcc gctttggcga ccgccgggct atgccctcgc
     9661 ctcccagaca aggacctcgc ccttgtcgga gacggccagg tcgcccgaat agtggcggta
     9721 tgctccatcg gccgggccgg gaagggtgac gccaaatggg cggaggtggt tgtccagcag
     9781 gcggataaac agcggatgga ccttcgccgc gaatgcaaag gttggcatca gctggagtgg
     9841 cttcagggaa atgatcgtgc cgcggttcat ccacgcgccc gtgcggattt ccgcgccgct
     9901 ttccaggacg attgtgcccc ccttcatttg cagtccggtg aagtccctgg ccgggccgcg
     9961 gatcacgatc gtgccgcgcc gcatccgcat gccgacttcc agtccggccg agccgtccac
    10021 gatgatcgtg ccgccgcgca tgccgcgcag cgagccgcgg taggccgcgc cgacctggcc
    10081 gccggcgctg ccgtggatgt ggatgaagcc gtttttcatc tcggcgccga tccagtcggt
    10141 cgcgttcccg tggacctcga tccgtccgcc ggacatgtac gcccccaggt gcatgcccac
    10201 cgagccgtgg atggtgatgc tgccctgcga catcgcgcgg ccgacccagc ggaccttgtg
    10261 cagcgcgccg tggaggatga ggtcactgct tttctcgccc tccacgtcga agaactcgcg
    10321 gagcggaagc tgccgcttgc cgtgatagac agtcagggtt cggatttcgg cgttggacag
    10381 ccccgccacg acatcgggcg acaggacttc agcttccaga ggaaccgtcg gcgggcgctt
    10441 gaggtgaaag acgatgggca tgggcagcaa acggccgaac taggcccgga gggccgacag
    10501 gcagtagccc cgaccgtaag gtcggggtac cgaatacgca agacgattca aggccccgag
    10561 ggccggcaca agaattgagg atgtgtgttg caacaggtgt cggcccctcc cgggcctaag
    10621 aatgcgttgt atttgtccgc gatccccgac ctgacggtcg gggctattac ctttcggccc
    10681 tccgggccta tttgccgact ggaacgcact cgcgcagctc ccggtgttcg atccgctcga
    10741 tctccactgg ataattctca aacgacatgg tgtagcaatc ctcgaacagg ggccgcatga
    10801 actcgttggt cgccggatcc atggccgggc ggacgaggaa ctcgcggccc tgcggggctt
    10861 cgcggatctc gccgtcctcg agcacgatct cgcccccctt gatcacgtac cgcgggtggc
    10921 tgaacatctg ggcgatgttc tcgtcctcgt gatacagcac caggtcggcg tcgctgccca
    10981 cgctgaggtt gcccttctgc gccaggccca gggcccgcgc ggggccggcc gacatggcgg
    11041 tcgcgatctc cagcagcgtg tactcgcggt cgatttccgg cagcgtgatg cggcccttga
    11101 tcttgtccgg cagccgcttg agttgctcct tccgcacgtc gcgccgcatc agcagctcga
    11161 tgatctcggg ataccgccag aagcagccgc cgttgggatg gtcggtcgag agaaagacct
    11221 gccaggggct cttgatgagc aagagcagct ccaggcccgc cgcccattgc accgcattca
    11281 ccaggttgct ggggcggtag gtgtagggca cgatgccgca gccggtct
//
LOCUS       DASRJB010000685         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2166529, whole genome shotgun sequence.
ACCESSION   DASRJB010000685 DASRJB010000000
VERSION     DASRJB010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166529"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..214)
                     /locus_tag="VFB80_18210"
     CDS             complement(5..214)
                     /locus_tag="VFB80_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35772.1"
                     /translation="MLQIALIAVSIGLVILGIKGFTANGIPVTKNFALKGRSGRVAGV
                     ICILTGIGFIPLFVLAFLGYMRWLT"
     gene            complement(244..1584)
                     /locus_tag="VFB80_18215"
     CDS             complement(244..1584)
                     /locus_tag="VFB80_18215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HZN35773.1"
                     /translation="MVPFSSTRSAGSGTCAQAAAGKTRSSIARRPLRSMNVRMGGREL
                     GAANRRAVKAQSRASPRLLHPRRPLVLMCIRRGFCRQAILMAFVPLAGPLLANAPARA
                     GDETSAQTANTYVYVSMAPEQKIQVYRLNAADGSLAAVETLPVEGTPGALAVDPRKKF
                     LFASHRNTSTLASYQIDPATGKLRRLSTAQLPMGQNAANLSTDRTGRWLLSASYAGGT
                     VVVHPLSEDGMIQTPAVQTITTAPTAHCIAIDRENRFVLVPHVAPNAVFQFRLDAAKG
                     QLSEAGKAPGGMDKAGPRHLAFHPTKNIAFTSDEQGSSITSYAYDPAIGLKPQQTLST
                     LPAGFTGQNTTADVKVHPSGKFVWVSNRGHDSLAGFAVDGSGQLSLLGHTPTEKTPRS
                     FEIDPSGRIALGAGEGSGKLAVFQIDPDTGQLTRLRTLDVGQSLTWVLAVKLDR"
     gene            1481..>3022
                     /locus_tag="VFB80_18220"
     CDS             1481..>3022
                     /locus_tag="VFB80_18220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN35774.1"
                     /translation="MLRSGLLAMLLLVLPAAAWAQVPEPADLVLLNGTIVTMVREGDV
                     AEAVAVRGGKIAAVGPAKEIRPHIGPKTKVVDLTGKTLLPGFFAAHDHFPSVGRVALY
                     DVDLNSPPIGTIRTMEELVAALREKAARTPAGQWVVGRGYDDTLLAEQRHPTRQDLDR
                     ASADHPIWIVHTSGHLGVANSRALALAKIDKDTPQPAGGAIRKDAAGEPTGVIEERTG
                     LVNRLVPGLSQEQRLAAVKLCDGQYLAKGVTTTVIAGGAANVIPDLLAAHDRGWLHLR
                     ALAMLSPPTGEPPTLAELAGRSPIPQRVRYSGVKILQDGSLQGYTGYLTAPYFTQPEG
                     RSVTDGGLRWSGYALRPREKLVELVGQFHRAGYQIAIHANGDAAIDDVLTAFRVAQAE
                     HPRADARHRIEHCQTPREDQLDQMKELGITPSFFVGHVYYWGDRHQRLFLGQQRAERI
                     SPLASAAKRGIRFTIHNDTPVTPVDPLLLVWCAVTRTTKAGQTLGPEQRITAYAALRA
                     VTIDAA"
BASE COUNT          516 a         1005 c          994 g          507 t
ORIGIN      
        1 ccagtcaggt gagccatctc atgtatccca gaaacgcaag gacaaacagc gggataaatc
       61 cgatgcctgt caggatacag atgacgccag caacccttcc cgacctgccc tttagagcga
      121 agttcttggt cacggggatg ccgtttgccg tgaacccctt gattcccaaa ataaccagac
      181 cgatcgagac ggcgatcaga gcaatttgaa gcacggcgca ttctccgact ggattgatcc
      241 acctcagcgg tccagcttga ccgccagcac ccaagtgaga ctttggccga cgtcgagggt
      301 gcgcaggcgg gtgagctggc cggtgtctgg atcgatctga aagacggcca gttttcccga
      361 gccctcgccc gcgccgaggg cgatgcggcc gctcggatcg atctcgaacg agcggggcgt
      421 cttctcggtc ggcgtgtgtc ccagcagcga gagctggccg ctgccgtcca cggcaaatcc
      481 cgccaggctg tcgtggccgc gattggagac ccacacgaac ttgccgctcg gatgcacttt
      541 gacgtcggcc gtcgtattct ggcccgtgaa gccggccggc agagtcgaga gcgtctgctg
      601 cggcttcagt ccgatggcgg gatcgtaggc ataggaggtg atgctgctgc cctgttcgtc
      661 agaagtgaag gcgatgtttt tcgtgggatg gaacgcgagg tggcgcggcc ccgccttgtc
      721 catgccgccc ggggccttgc cggcctcgct cagctggccc ttggcggcgt ccaggcgaaa
      781 ctggaagacc gcgttgggag cgacgtgcgg caccaacaca aaacggttct cgcggtcaat
      841 ggcgatgcaa tgcgccgtcg gcgcggtcgt gatcgtctgc accgcgggag tctggatcat
      901 cccgtcctcg ctcagcggat gcacgacgac cgtgccgccg gcgtacgaag ccgacagcag
      961 ccagcggccg gtgcgatcgg tgctcagatt cgccgcgttc tgccccatcg gcagttgagc
     1021 ggtgctaagc cgccgcagct tgcccgtggc cggatcgatc tggtagctgg ccagcgtgct
     1081 ggtgttgcgg tgcgaggcga acaggaattt ctttcgcgga tcgacggcca gggcgccggg
     1141 cgttccttcg accggcagcg tttccaccgc agctagcgac ccgtcggccg cattcagtcg
     1201 atagacctga atcttctgtt cgggggccat cgagacgtaa acatatgtgt tcgcagtttg
     1261 ggcactggtt tcgtcgccag cgcgagcggg ggcgttcgcc agcaacggtc ccgcgagcgg
     1321 aacaaaggcc atcaagatcg cttgccggca gaaacctcgt cggatgcaca ttagcacgag
     1381 cggcctccta ggatgtagca gtcgaggaga tgccctagat tgtgccttga cggcacgccg
     1441 attggcagcc cccaattctc gcccacccat gcgcacgttc atgctacgca gtggtctcct
     1501 cgcgatgctg ctcctggttt tgccggcggc agcttgggca caagtcccgg agcctgccga
     1561 cctggtgctg ctgaatggga ccatcgtcac gatggtccgc gaaggggacg tcgcagaagc
     1621 cgttgccgtt cgcggcggca aaattgcggc tgtcgggccg gccaaagaga ttcgcccgca
     1681 catcgggccg aagaccaagg ttgtcgatct gaccggcaag acgctgctgc cgggattctt
     1741 tgccgcgcac gaccactttc cctcggtggg ccgcgtggct ttgtacgacg tggacctgaa
     1801 cagcccgccg atcggcacga tccgcacgat ggaggagttg gttgccgcgc tccgcgaaaa
     1861 ggcggccagg acgccggccg gccagtgggt cgtgggccga ggctacgacg atacgctgct
     1921 ggccgagcag cggcacccga cgcggcagga cctcgaccgc gccagcgccg accatccgat
     1981 ctggatcgtt cacacctcag gccacctcgg cgtggccaac tcgcgggccc tggccctggc
     2041 aaaaattgac aaggatacgc cacagccggc cggcggcgcg attcgcaagg acgccgccgg
     2101 tgagcccacg ggcgtgatcg aagaacgcac cggcctggtc aatcgcctcg tccccggcct
     2161 ctcgcaggag cagcggctgg cggccgtgaa actctgcgat ggccagtacc tggccaaggg
     2221 cgtcaccacg acggtcatcg ccggcggcgc ggccaatgtc atccccgatc tgctcgcggc
     2281 gcacgatcgc ggctggctgc acctgcgggc gctggcgatg ctctcgccgc cgaccggcga
     2341 gccgccgacg ctggcggagc tggccggccg cagcccgatt ccccagcgcg tgcgctatag
     2401 cggcgtaaaa atcctgcagg acggctcgct gcagggctac acgggctatc tcaccgcgcc
     2461 ctatttcacg cagccggaag gccgcagcgt tactgacggc gggctgcgct ggtcgggcta
     2521 tgccttgcgg ccgcgggaga agctggtgga gctggtcggg cagttccacc gcgccggcta
     2581 ccagattgcg attcacgcta acggcgacgc ggcgatcgac gacgtgctga cggcgtttcg
     2641 cgtcgcgcag gccgagcatc cgcgggcgga cgcccgccac cgcatcgagc attgccagac
     2701 gccgcgcgag gaccagctcg accagatgaa ggagctgggc atcacgcctt ccttctttgt
     2761 cggacacgtc tattactggg gcgaccggca ccagcggctg ttcctcggcc agcagcgggc
     2821 cgaacggatc agcccgctgg cctcggccgc aaaacgcggc atccgcttca cgatccacaa
     2881 cgacacgccg gtgacgccgg tcgatccgct cttgctcgtc tggtgcgctg ttacgcgcac
     2941 caccaaggcc ggccagaccc tcggccccga gcagcggatc actgcctacg ccgccctccg
     3001 cgccgtcacg atcgacgccg cc
//
LOCUS       DASRJB010000686         9753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1325350, whole genome shotgun sequence.
ACCESSION   DASRJB010000686 DASRJB010000000
VERSION     DASRJB010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325350"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2470
                     /locus_tag="VFB80_18225"
     CDS             <1..2470
                     /locus_tag="VFB80_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35775.1"
                     /translation="VVARFFRSSSATDYGDALAWLLAASVPLLAATLNQFDGLGRWFA
                     YHTLEAGWLAVAAIACVAVCSRQWLGERLARIAPHHFSATAIAVLVVFLAIRGDLQDP
                     GQPWWSLAGALGCAAIATTLGLARRSQPYAFAATALAGLAVLLFWSTRIAGRWVSVVM
                     DDFYWFVAWETFFIALVAAAGFWLWREIDSQLRRGESLDARRFMPRVHAGVICGLVPL
                     LFLWRLTYGLFSTWQGGRGLNFDIAAAVTTVVLGGLLAATLWDRRATWLGPLTFLFGG
                     AAWCLAIGLLRFWLPLPECKLTALVLALAGQVALSGQIWSYGANLAAWGTKAGVPDPI
                     GGLSRTAKWLPVVNLLLTCLGCLASLLLVLTLDTRKEILPFRVAAAFGPAIAAWGVAC
                     LAQERRREALQLTALLVAGFSAVLLGWSQIDGHTADLWMQRVFRLLMVLAALTFAYGL
                     VLPRLLLTGGSWHAATRKAGYIAGSAAVAAFVVTLALEISLFVPGRGAAVGDVQVIAI
                     AVVLVALIAGLVSMALLPGRDPLLLSERAKQAYVYAAEAVGALLFAHLYVCKPLWFDT
                     VLRPYWPFIVMGLAFSGVGAAELFHRWKVRVLAEPLERTGALLPLLPVIGWWVAGSQV
                     DYALLLLVAGILYLALSFTRKSWAAMVAAAVAGNGALWSLFSEKAFHFADHPQLWLIP
                     PALSVLAAAQVNRHRLSAEALTAIRYAATIVIYVSSTSEIMLRGLGTDLRPPMILLGL
                     SVAGALAGIALRVRAFLYLGAAFTLLALTSMVWHASRSIEHVWPLWAFGICLGVGLLV
                     MLVLFEKNKAEMHQLVARLRKWEQ"
     gene            complement(2488..3321)
                     /locus_tag="VFB80_18230"
     CDS             complement(2488..3321)
                     /locus_tag="VFB80_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HZN35776.1"
                     /translation="MAARPRILMCPPDHYGIEYEINPWMNRQRQADQPTAARQWRALH
                     DLLLRLGAEIPLLDPVPGLPDLVFTANAALIYRRRAILAHFRHAQRQGEEPLCAAWLQ
                     KHGLAVERLPEERFFEGAGDALFCGETLIAGYRIRSDVRSQQIIGELLGCEVIPLELI
                     DGHYYHLDTCFCPLAPGEAIWYPGAFDDYGRRVLAELIPDLVVVAPEEARSFACNAVV
                     VGKNVVTTTGCPQLHKGLAERGYTPHETPLSEFIKSGGSAKCLTLRLDGEEAAAWKTI
                     R"
     gene            3440..4459
                     /locus_tag="VFB80_18235"
     CDS             3440..4459
                     /locus_tag="VFB80_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZN35777.1"
                     /translation="MSAWSRRRFLLIAAGGAAAAGGASSFLAGGGQTWPAVTRTTKAL
                     GTRVALTVRDRRADEAELAIADAFEELALVERLMSIYRPDSQLSRLNATGELFDPHPH
                     LLAVLSHAQRTSQQSGGAFDVTVQPLWEVFAAAQKQQRLPSAAEVAAARSRVDWRQIE
                     IAERRVRLLRPGTKITLNGIAQGFAADRVRSVLEEHGIQHALIDAGELNSIGHSPRAS
                     AWRVGIQHPRDPEAYIALADLDGRALATSGDYATRFSPDGYYNHILDPRSGYSPTEVA
                     SVSVLAPSATAADALSTTLFVLGEQRGLRLLAAQRHTDMFMVLKSGETIATPGFPLRA
                     AGRAG"
     gene            4459..5802
                     /locus_tag="VFB80_18240"
     CDS             4459..5802
                     /locus_tag="VFB80_18240"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZN35778.1"
                     /translation="MPGVKNVGWIAALVLVVASAASSPAATIEYLDGTRIECQVLSKD
                     ATSVTIEAKSAGMLVKRTIPLAKIHKVTINDKVYVINERAAAGAAKSAPAGKSKAAGP
                     LESGESAGGKELRTKAQVDALIDELGRQPPDWYEATPLNHPQSLDLSWPDGPPPGGWN
                     NQKNVGQFVWDIVNPNPGKWREGVKLMHHLLTLHKDDPAKRTRIMVELGRMYHNLHQD
                     YARAAFWWRQAGVEKTNSPPGVTVHLAECFWRLGNKQMALDHLRKLKSIPYDAIKLLA
                     DMGEAPEALRLVESIAKNPNNQLHFAYLYGGDACRVAGRYAEAVKYYEKVLAIAAEGQ
                     QKGQIDRCQKRAAESLAAIKLFELLDVQKIADGKYRSSAIGYEGPIEVEVSVAGGKID
                     AVRITQHREKQFYSAMTDTPAKIIAKQGVKGVDATSSATITSEAIINATAKALAK"
     gene            complement(5851..7581)
                     /locus_tag="VFB80_18245"
     CDS             complement(5851..7581)
                     /locus_tag="VFB80_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35779.1"
                     /translation="MRASRWLIAAVALVATGFTASLGFAQRGDRGDYGRGDSGRGDYG
                     SRGGGDFGSRGFGGGFGGSPFGGGGFGGFGGGGMFGGGMGGYGGGYGMSRDPRDMLRQ
                     ADLNGNGVIEPAEMQGRSGWMAQRMAERAGLDTNSPLPIDRLASAMDGSSRDSRDSRG
                     SSSSSGSAQTPVSAMSFGIVGEVKRVPGFDVPLGAAEQVPLEKRFDQRVYERAKELLE
                     DRDRNKNGTIEGEELTRNNWDPPADTSDLNKDGSLNLEELCYRYQARYGGGPPQGGPN
                     NGARMQVAVIPPPLFPSSAPATGSAATPGSPGGDERLKKWAEGMLTRADTNKSGKLEK
                     DEWGGVRDAENIDTSKDGIITVEELMASLQAFAGGRRDGGGEGGHRDGGGEGGRGWGE
                     GGGGRSFGGGEGGRGFGFGGEGGGEFGGRGRGGRGFGGGGPFGGGGEMVINVGGGENS
                     GGENGNRNGDGNRNGENGRNGGRGNGSSNSATTTPSATTTPAPKSYRLASPWERLPEG
                     LSDRFYNLDINEDGQVSMSEYRASSGWSDQDAERFLNYDQNRDGLISAKEWLEGEGGG
                     GERSRSRGRN"
     gene            complement(7820..8344)
                     /locus_tag="VFB80_18250"
     CDS             complement(7820..8344)
                     /locus_tag="VFB80_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN35780.1"
                     /translation="MPSVCPFCDSEVIPGSDSCDTCGQSLTESHLVPPTTTIEQAILS
                     DRVRLFQGRPPLVVSPTMPVREVLRLMVDNRVGCIVIADQGKLAGIFSERDVLMKLGE
                     RASELGGRPVSEFMTSKVESLPPTAKIAFAVQRMDQGGYRHIPIVNDKGEATGIFSVR
                     DILSYMAKKMAGGK"
     gene            complement(8834..9523)
                     /locus_tag="VFB80_18255"
     CDS             complement(8834..9523)
                     /locus_tag="VFB80_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35781.1"
                     /translation="MRTILVLSVLLVTSIATAQNAPKREPPNIAQSSANVVRDRKIFD
                     EHPQFLTMVRNSRAIQAELGITDTQIREIDEKYSLGREVVSAGFKALEGLPDEQKGAA
                     YQRIFEDDMASHRPLIKSILTQTQMVRLEQIALQQRVKTFYPAAGLTHPLLVKALEIS
                     ADQQQEIRSHVEKVEAKFKARIAELNADMTKVRDDARQEALTKLTPEQRRVYQSLIGK
                     FFDGDAPVERR"
     gene            complement(9561..>9753)
                     /locus_tag="VFB80_18260"
     CDS             complement(9561..>9753)
                     /locus_tag="VFB80_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35782.1"
                     /translation="WVEADTYDELIAVASSTPTPATSPPCQLLYYAMVQVQYHDGTTF
                     VNLDWVPMDEFWQYPGDPE"
BASE COUNT         1606 a         3187 c         3126 g         1834 t
ORIGIN      
        1 ggtcgtggcc cgtttcttcc gtagcagcag cgctacagac tacggcgacg cgctcgcctg
       61 gctgctggcg gccagcgtgc cgctgctcgc agccacgctg aatcaattcg atgggcttgg
      121 acgctggttt gcctaccaca cgctcgaagc cggttggctg gccgttgccg cgatcgcctg
      181 tgtcgccgtg tgctcgcgac agtggctggg cgagcgcctg gcacgaatag caccgcacca
      241 tttctcggcg acggcgatcg cggtgctggt cgtgttcctt gccatccgcg gcgacttgca
      301 ggatcccggt cagccgtggt ggtcgctggc tggcgcgctc ggctgtgcgg ccatcgccac
      361 tacgttgggc ttagcgcggc gcagccagcc gtatgcgttc gccgcgacgg cgctcgccgg
      421 cctggcggtc ctgctgtttt ggagcacgcg catcgcggga cggtgggtca gcgtcgtcat
      481 ggacgatttc tactggttcg tggcctggga aaccttcttc atcgccctcg tcgcggctgc
      541 cgggttttgg ctgtggcgcg aaatcgattc gcagctgcgc cgcggcgagt cgctcgacgc
      601 ccgccgattc atgccgcgcg ttcacgcggg cgtgatttgc ggattggtgc cgctgctctt
      661 tctgtggcgg ctgacctatg gattgttttc cacgtggcag ggcgggcgcg gtctgaattt
      721 cgatatcgcg gctgccgtga cgaccgttgt gctgggcgga ctgctcgccg cgacgctgtg
      781 ggaccggagg gcaacctggc tcgggcccct gacgtttctc ttcggcggcg cggcctggtg
      841 tctggcgatc ggactgctcc ggttctggct cccgctgccc gagtgcaagc tgacggcgct
      901 ggtgctggcg ctggcgggcc aggtggcgct gagcggccaa atctggagtt acggggcaaa
      961 cctggcggcg tgggggacca aggcgggggt gcccgatcca attggaggat tgtcgcggac
     1021 ggccaagtgg ctgcctgtgg tcaatctgct cctgacctgc ctgggttgcc tggcgagctt
     1081 gctgctggtg ctcacgctcg atacgcggaa ggaaatcctg ccgtttcgcg tggcagccgc
     1141 cttcgggccg gccatcgcgg cctggggcgt ggcgtgcctg gctcaggagc ggcggcgcga
     1201 ggcgctgcaa ctgacggcgc tgctcgtcgc aggtttctcg gcagtgctgc tcggctggtc
     1261 gcagatcgac gggcatacgg ccgatttgtg gatgcagcgc gtcttccgcc tgctgatggt
     1321 gctggcggcg ctgacgtttg catacggtct ggtgctgccg cgactgctct tgaccggcgg
     1381 ctcgtggcac gcggcaacgc gaaaggccgg ctacatcgcc ggcagtgcgg cggtcgccgc
     1441 gtttgttgtg acactggccc tggagatctc gctgttcgtg ccgggccgcg gtgcagctgt
     1501 cggcgacgtg caggtgatcg cgatcgcggt ggtgctggtg gccctgatcg ccggtctggt
     1561 gtcgatggcc ctcttgcccg gccgcgatcc gctgctgctc tctgagcggg cgaagcaggc
     1621 ctacgtttat gcggccgagg ccgtcggggc gctgctcttc gcgcacctgt atgtctgcaa
     1681 gccgctctgg ttcgacacgg tgctgcggcc ctattggccg ttcatcgtga tggggctggc
     1741 gttttccggg gtgggagcgg ccgagctgtt tcaccgctgg aaagtccgcg tgctggccga
     1801 gccgctggag cggaccgggg cgctcttgcc gctgctgccg gtcatcggtt ggtgggtggc
     1861 tggctcgcag gtcgattacg cgctgctgct gctggtagcc ggcatcctct atctggctct
     1921 cagcttcacg cgcaagagct gggcggcgat ggtcgcggcg gccgtcgccg gcaatggcgc
     1981 gctgtggtcg ttgttcagcg aaaaggcgtt ccactttgca gaccatccgc agctctggtt
     2041 gatcccaccc gccctgtcgg tgctggccgc ggcgcaggtc aaccgccatc ggctgagcgc
     2101 cgaagccctg accgccatcc gctatgccgc gacgatcgtg atctatgtca gctcgaccag
     2161 cgagatcatg ctccgcggac tgggcacgga cttgcggccg ccgatgatcc tgctgggact
     2221 ttcggtcgcc ggcgcattgg cgggcatcgc cctccgcgtg cgggcctttc tctatctggg
     2281 cgcggccttc acgctgctgg ccctgaccag catggtctgg catgcctcgc gctccatcga
     2341 gcacgtctgg ccgctgtggg cgttcggcat ctgcctcggc gtcggcctgc tcgtgatgct
     2401 cgtgctgttc gagaaaaaca aggccgaaat gcaccagctg gtggcccggc tgcggaagtg
     2461 ggagcaataa gcagccgtgc gaaccggcta gcggatcgtt ttccaggcgg cggcctcttc
     2521 gccgtcgagt cgcagcgtga ggcacttcgc gctgccgccg gatttgatga actccgacag
     2581 cggcgtttcg tgcggcgtgt agccgcgctc ggccaggccc ttgtgcaatt gcgggcagcc
     2641 cgttgtcgtg acgacgttct ttccgacgac caccgcgttg caggcgaacg aacgggcctc
     2701 ctccggcgcg accacgacca ggtcgggaat caattcggcc agcacgcggc ggccataatc
     2761 gtcgaacgca ccgggatacc agatcgcttc gcccggcgcg agcgggcaaa agcaggtgtc
     2821 gaggtgatag tagtgcccgt cgatcaattc cagcgggatg acctcgcagc cgagcagctc
     2881 gccgataatc tgctggctgc ggacgtcgct gcggatgcga tagcccgcga tcagcgtctc
     2941 gccgcaaaac agcgcgtccc ccgcgccttc gaagaaccgt tcctccggca gccgctcgac
     3001 cgccaagccg tgcttctgca gccaggcggc gcagagcggc tcttcgccct gccgctgcgc
     3061 gtggcgaaag tgggccagga tcgcccgccg gcgatagatc agggcggcgt tggccgtgaa
     3121 caccaggtcc ggcaggcccg gaaccggatc gagcagcgga atctcggccc ccagccgcag
     3181 cagcaaatcg tgcagcgccc gccactgccg cgcggccgtc ggctgatcgg cttgccgctg
     3241 ccggttcatc cagggattga tctcgtattc gatcccgtaa tgatcgggcg ggcacatcag
     3301 gatgcgcggg cgggcggcca cgggcggttc tccgtttcgt ccaaagaatg gtcgcggcaa
     3361 agcgcttgcg agacaagggc ggccgtgcgt aaagtacgag cggagaactg gttaggccgt
     3421 aggaagcgaa gaggccaaca tgagcgcgtg gagtcggcgg cgattcttgc tgatcgccgc
     3481 gggaggcgct gccgctgcgg gcggcgcgag cagttttctg gcaggcggtg ggcagacctg
     3541 gcccgccgtc acgcgcacga ccaaggcgct gggcacgcgc gtcgcgctca ccgttcgcga
     3601 ccgccgcgcg gacgaggccg agctggcgat tgctgacgca ttcgaggagt tggcgctggt
     3661 cgagcgattg atgagcatct atcggccgga cagccagctc agccgcttga acgcgaccgg
     3721 cgagctcttc gatccgcatc cacatctgct cgccgtcttg agccatgccc agcggacgag
     3781 ccagcaaagc ggcggcgcct tcgacgtcac cgtgcagccg ctgtgggaag tgtttgccgc
     3841 cgcacaaaaa cagcagcgct tgccttccgc ggcggaagtc gccgccgccc gctcgcgggt
     3901 cgattggcgg caaatcgaaa tcgccgagcg ccgcgtccgc ctgttgcggc cgggcacgaa
     3961 aatcacgctc aacggcatcg cccagggatt tgccgccgac cgagtgcgga gcgtgctcga
     4021 ggagcacggc atccagcacg ccctgattga tgcgggcgaa ttgaattcga tcggccattc
     4081 gccgcgcgct agcgcctggc gagtgggcat tcagcatcca cgcgacccgg aggcctacat
     4141 cgccctggcc gatctcgacg gccgcgcgct ggccacctcg ggcgactacg cgaccaggtt
     4201 ttcgccggac ggctattaca accatattct cgatccccgc agcggctatt cgccgacgga
     4261 agtcgccagt gtgtcggtcc tcgctccatc ggccacagcg gctgatgccc tttcgacgac
     4321 cttgttcgtg ctaggcgagc agcggggact gcggctgctc gccgcacagc gccacacgga
     4381 catgttcatg gtcctcaaga gcggcgagac gatcgccact cccggctttc cgctccgcgc
     4441 ggcggggagg gccggctgat gcctggagtc aaaaatgtcg gttggatagc cgcgctggtg
     4501 ctggttgtcg ccagcgcagc cagctcgccg gccgcgacga tcgagtatct cgacggcacg
     4561 cggatcgaat gccaggtgct gtcgaaggac gcgaccagcg tcacgatcga ggccaagtcg
     4621 gcgggaatgc tcgtcaaacg cacgattccg ctggcgaaga ttcacaaggt gacgatcaac
     4681 gacaaggtct atgtcatcaa cgagcgggcg gcggccggcg cggccaagag cgcgccggcg
     4741 ggtaaatcga aggccgccgg cccgctcgaa tcgggcgaga gcgccggcgg aaaggagctg
     4801 cggacgaagg cgcaggtcga tgcactgatc gacgagctcg gccggcagcc gccggattgg
     4861 tacgaggcca cgccgctgaa ccatccgcaa tcgctggacc tgtcgtggcc cgacggcccg
     4921 ccgcccggcg gctggaataa ccaaaaaaac gtgggccagt tcgtgtggga catcgtcaat
     4981 cccaaccccg gcaagtggcg cgagggggtc aagctgatgc accacttgct cacgctgcac
     5041 aaggacgatc ccgccaagcg cacgaggatc atggtcgagc tcggtcggat gtatcacaac
     5101 ctgcaccagg attacgcccg ggccgccttc tggtggcggc aggccggcgt cgagaagacc
     5161 aattcgccgc cgggcgtgac cgtacacctg gccgagtgct tctggcggct gggcaacaag
     5221 cagatggccc tcgaccacct gcggaagctg aagtcgattc cctacgacgc gatcaagctg
     5281 ctggccgaca tgggcgaagc cccggaggcc ctgcggctgg tcgaaagcat cgccaagaat
     5341 ccgaataatc agctgcattt cgcgtatctg tatggcggcg acgcctgccg cgtggccggc
     5401 cgctacgccg aggcggtgaa gtactacgaa aaagtgctgg cgattgccgc ggagggccag
     5461 cagaagggcc agatcgaccg ctgccagaag cgggcggcgg agagcctggc cgcgatcaag
     5521 ctgttcgagc tgctcgatgt gcagaaaatc gccgacggca agtaccgctc gtcggcaatc
     5581 ggatacgaag gaccgattga agtcgaagtc tccgtggcag ggggcaagat cgacgcggtc
     5641 cgcatcacgc agcaccgcga aaagcagttt tattcggcga tgaccgacac gccggccaag
     5701 atcatcgcca agcaaggcgt gaaaggcgtg gacgccacca gcagcgccac gatcaccagc
     5761 gaggcgatca tcaacgcgac cgcaaaggcg ctggccaagt gaattcggaa ttcagaaggc
     5821 agaattcgga attacgatca gaatctcgca ctaattccgc ccacggctgc gggagcgctc
     5881 gcctccgccg ccctcgcctt cgagccattc cttggcgctg atcagcccgt cgcggttctg
     5941 gtcgtagttc aggaaccgct cggcgtcctg gtcgctccag ccggagctgg cgcggtattc
     6001 cgacatcgag acctgtccgt cctcgttgat gtcgagattg tagaaccggt ccgagagccc
     6061 ttccggcaga cgttcccatg gcgaggccag gcggtagctc ttgggcgccg gcgtggttgt
     6121 ggcgctaggc gtggttgtgg cgctattcga agatccgttg ccgcgcccgc cattgcggcc
     6181 gttttcgccg ttgcgattgc cgtcgccgtt gcggttgccg ttttcaccgc cgctattctc
     6241 gccgccgccg acattaatca ccatttcgcc gccgccgcca aacggtccgc ccccgccaaa
     6301 cccgcgacca ccgcggccgc ggccgccgaa ttcaccaccg ccttcgccgc caaatccaaa
     6361 gccgcggccg ccttcgccgc cgccgaagct gcgtccgcct ccgccttctc cccagccgcg
     6421 tcctccttca ccgcccccgt cgcggtgtcc gccctcgccg ccgccgtcgc ggcgaccacc
     6481 ggcgaaagcc tggaggctgg ccatgagttc ctcaactgtg atgatgccgt ccttgctcgt
     6541 atcgatgttc tcggcatccc gcacaccacc ccactcgtcc ttctcgagct tgccgctctt
     6601 gttcgtgtcg gcacgagtca acatgccctc ggcccatttc ttcaggcgtt catcgccgcc
     6661 gggagagccg ggtgtggccg cgcttccggt ggccggagcg ctggagggaa agagcggcgg
     6721 cggaatgacc gcgacctgca tccgcgctcc gttgttggga ccaccttgcg gtggaccgcc
     6781 gccgtagcgg gcctgatagc gatagcacaa ctcttccagg ttcagtgagc cgtctttgtt
     6841 cagatcgctc gtatcggccg gcggatccca attgttgcgg gtcagctcct cgccctcgat
     6901 cgtgccgttc ttgttgcggt cgcgatcttc cagcaattcc ttggcccgct catacacccg
     6961 ctggtcgaac cgcttttcca gaggcacctg ctccgcggcc cctagcggta cgtcgaaccc
     7021 gggaacccgc ttgacctcgc ccacgatgcc aaatgacatc gccgacactg gcgtctgcgc
     7081 cgaaccactg ctcgacgaac tcccccggct gtcgcggctg tcccgcgagc tgccatccat
     7141 cgcgctggcc agccggtcga tcggcagcgg gctgttcgtg tccaatccgg cccgttcggc
     7201 catccgctgg gccatccagc cggagcggcc ctgcatctcg gccggctcga tgaccccgtt
     7261 gccgttcaag tcggcctgcc gcagcatgtc gcgcggatcg cggctcatgc cgtaaccgcc
     7321 gccgtagccg cccatgccgc cgccaaacat cccgccgccg ccaaagccgc cgaagccgcc
     7381 gccaccgaag ggactgcccc cgaatccgcc gccaaagccg cggctgccaa aatcgccgcc
     7441 gccgcggctg ccataatcgc cgcggccgga atcgccgcga ccatagtcgc cgcgatcacc
     7501 tcgctgggcg aagcccaaac ttgccgtgaa accagttgcc accaaagcaa cagcggcgat
     7561 cagccagcgg gaagctcgca tagcggtcac tccatcaatg catgcatact cggcgttttt
     7621 tcccgaacgg catattctat cgcaagtcat tacgttgcaa tgacttgcga tcattccacg
     7681 agatgcattt tatgcacttt atgcaggata agcatgggtg cgtgcatcac cgccgcccgc
     7741 tcgagggcgc attctaccaa ccacttaacc ccgaattcca gcgaaagttg ctgtggacgt
     7801 cgccagtttc cccgggaatt tacttgccac ccgccatctt cttggccatg tagctcaaga
     7861 tgtcgcggac ggagaagatg ccggtggctt cccccttatc gttcacgatc ggtatgtggc
     7921 gatagccgcc ctgatccatc cgctgaacgg cgaaggcgat ctttgccgtc ggcggcagcg
     7981 attccacctt gctggtcatg aattcggaca cgggtcggcc gcccagctcg ctcgcccgct
     8041 cgcccagctt catcagcacg tcccgctcgc tgaagatccc cgccagcttg ccctggtcgg
     8101 cgatgacgat gcaccctacg cggttatcga ccatcagccg cagcacctcg cggacgggca
     8161 tcgtcggcga aacgactagc ggcggccgtc cctgaaacag gcggacgcgg tccgagagaa
     8221 ttgcctgttc gatcgtcgtc gtggggggga ccaggtgcga ttccgtcagc gactggccgc
     8281 acgtgtcgca ggaatcactg ccgggaatga cctcgctatc gcaaaaggga caaacagacg
     8341 gcatggcaaa cccccggcgc gaatgcggag tcgaatgacg agcaatggga gcagtgtacg
     8401 cgggcgacca cccgtagaac aagcgtcctc gcttgttcac gctagccgta gaataaagcg
     8461 tcctcgcttg ttcaccctga gcaagcgagg ccgcttgctc tgcaccagca attcgcctcc
     8521 tcttgctccc aagctcccag ctcccgagct ccttgctccc aagctctgct tgcgaaccag
     8581 aaccaaaaac tcgcgcggga ggccgcaaac gctttaatgg cttgcgcccg aatgctatgg
     8641 gcggcgtgcc gcaagtgctt gggggccaat gcgttgcggc gcgattttcg cgcggaagct
     8701 ctcaaaaaga tcctcgagcg cggcagtttt cttatattcg ccgcctgacg cttgaggcct
     8761 gttgaattgg cctatcgaac cagacgagct gactgaaatg attgccaagg ggtagcagat
     8821 tattgtgcag ctttcatcgt ctctccaccg gcgcgtcgcc gtcgaagaac ttgccgatca
     8881 gcgattgata cactctgcgc tgctccggag ttagcttagt aagcgcttcc tggcgggcat
     8941 catcgcgaac cttagtcata tctgcattca gctccgcaat acgtgccttg aacttggcct
     9001 cgaccttctc aacatgcgat cggatttcct gctgctggtc ggcgctgatc tctagcgcct
     9061 tcacgagcag gggatgcgtc agaccggcgg ccgggtagaa cgtcttgacc cgctgctgta
     9121 gcgcgatctg ctcgagcctc accatttgtg tctgggtaag tatcgacttg attaacggcc
     9181 ggtggctcgc catgtcgtcc tcaaatatcc gctgatacgc tgcgcccttc tgctcatccg
     9241 gcagtccctc caacgccttg aaacctgcac tcaccacttc gcggccgagg ctgtatttct
     9301 cgtcgatctc ccgaatctgg gtatcggtaa tgcccagttc ggcttgaatc gcacggctat
     9361 tccttaccat ggttaggaac tgcgggtgct cgtcaaatat cttgcggtcg cggacgacat
     9421 tcgctgaact ttgggctatg ttcggtggct cacgcttcgg agcattctgc gctgtcgcga
     9481 tacttgtaac caacaggacg gacaatacta agatggtgcg cacggcttcc tcctcgctaa
     9541 caagatgaag taaatgccgg ctactctggg tcccctgggt actgccaaaa ctcgtccatg
     9601 ggtacccagt ccaggttgac aaaggtggtt ccgtcgtggt attgcacctg caccatcgcg
     9661 taatagagaa gctgacacgg aggagaggtg gcaggcgttg gggtcgagga ggcaactgca
     9721 attaattcgt cataagtgtc agcttcaacc cac
//
LOCUS       DASRJB010000687         7248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1889167, whole genome shotgun sequence.
ACCESSION   DASRJB010000687 DASRJB010000000
VERSION     DASRJB010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889167"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFB80_18265"
     CDS             <1..361
                     /locus_tag="VFB80_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZN35783.1"
                     /translation="GEDAAARFLRKLGYVIVARGHRDNIGEIDLVAVDKRTVVFIEVK
                     TRTTHDAGHPADAVDDAKQRRLTRLALSYLKRHDLLECAARFDVVAVTWPAGRGKPTI
                     EHFVNAFEPVGFPGMFS"
     gene            complement(422..1111)
                     /gene="queC"
                     /locus_tag="VFB80_18270"
     CDS             complement(422..1111)
                     /gene="queC"
                     /locus_tag="VFB80_18270"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZN35784.1"
                     /translation="MAAQRPAVVLLSGGLDSATVLAIARSEVFACHALSFRYGQRHAI
                     ELTAAERVARQLGAVSHRIVEIDLRQFGGSALTDDIAVPKHASVGEIGGGIPLTYVPA
                     RNTIFLSFALAFAEVSGTADIFIGVNALDYSGYPDCRPEYIAAFERMANLATKAGVEG
                     RPLKIHTPLIDLTKAQIIRRGLDLGVDYSLTFSCYDPSAAGIACGRCDACLLRLKGFA
                     ENGASDPIPYA"
     gene            complement(1112..1957)
                     /locus_tag="VFB80_18275"
     CDS             complement(1112..1957)
                     /locus_tag="VFB80_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35785.1"
                     /translation="MEKWPIGVFASVDAGLGVKLEVARDLGVPTIQLHAPHPATRTPA
                     NAEAFLKKLAGFGIKLTCVFSGFEGESYADIPTVAKTVGLVPPGPRAERIKELKANAD
                     FAKLLGCSCVGLHIGFVPHDRSLPLYKEIVAVARDVCDYLAKNKQALHLETGQETADD
                     LLQFIQDVERNNLFINFDPANMILYGTGEPIAALKKVGRYVKSVHCKDGKWAKNIGKE
                     WGQEVPLGQGDVGMENYLRTLKEIGYDGPLTIEREIAQEPERQKTEIGGAIKLLTDLR
                     KKVLG"
     gene            complement(2143..3981)
                     /locus_tag="VFB80_18280"
     CDS             complement(2143..3981)
                     /locus_tag="VFB80_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35786.1"
                     /translation="MNNLTPEEREVGKENYYSAVSSFDQSPAADAWKRRDFLKTVLGV
                     GAAATAGVTTGCKYFGYSPIGDPVRVSVIGTGDEGSVLIGAINPDYIKVVSICDIRPY
                     NVHRAFHGDWAGDAAIAARPGLIAKYGFADEKAAKDAIKVESDYKKVLDDPNVEAVII
                     ALPLFLHAQVAVDALKAGKHVLTEKLMAHNVAQCKVMGRLAKKENKVLTVGHQRHYSV
                     LYDNAVNLIRWGLLGEMHHIRAQWHRGNLPGNDSWQQPLPFPVETYEDGVKQKEKRNF
                     VAEKLKDLQDKLKREKDALKKRLLEKQVAQWAAWVLDKDVEAAKYGYSDFKLSDGRMR
                     TALEELCRWRLFARTGGGLMAELGSHQLDASSIFVSAVRKDGKKAHPLSVHAVGGRHT
                     FPYDRDAEDHVYCTFEFPGPGYEPGYKAGYYDPEMNYPDPTKGILSYDEDPNKRIVVT
                     YSSIMGNGWGGYGEIVMGSKGTLILDREQEVLLFRESDTATKVGVKEDGKGATLDTQA
                     SGKGPSLARAAAESGPVSRGYTEEIEHFAWCIRNNAPDAPRCKAEVALGDAVIALTSN
                     VAIDRSLNGQGGFVQFKEEWYDINSDETPDGSNLKSENEQLQAGLS"
     gene            complement(3978..4889)
                     /locus_tag="VFB80_18285"
     CDS             complement(3978..4889)
                     /locus_tag="VFB80_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35787.1"
                     /translation="MTDSSYRIGWLAAAAVVLLRVGIGVHFLAEGVDKLADPKPFSGP
                     FFGAAKGPLADVYKGMVWDADGRFRLSLDIAKAQWDQFRSSIVTHYRFDEAQTKEAAD
                     VLKDYTQRLNDFLGVNQDKIDEYYQRLDRRDANAGDPTRNLASLQVHDAKINAELLKM
                     RGELLPTIEGLWRDFENDLNAIATDDQWRRHGRLSLGKPGRRSFDTVTLDWLVPYFDL
                     TIGVCLILGLFTRVAALAGAAFLAMVCTTQWPGSPGAIPVFYQAVEMLALLVLAAVGA
                     GKFLGIDYLLGGMKALCCPPKKQGAAK"
     gene            5011..6048
                     /locus_tag="VFB80_18290"
     CDS             5011..6048
                     /locus_tag="VFB80_18290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN35788.1"
                     /translation="MAGLPDRVASGEQLDDLLSEPTAGVIQTLGKLDGDLLVLGVGGK
                     MGPTLARMAVRASEQASVKRRVIGVARFSNRELPAWLARHGVEPLTADLLDPRQVAQL
                     PDAANVIVMTALKFGASGRPGDTWAVNCWLPANICQRFAGSRIAAFSTGNVYPLTPIA
                     RGGSLETDPLGPVGEYAASCVGRERMYDYFSRLAGTKISILRLNYACELRYGVLVDLA
                     QQILAREPIDLTMGAVNVIWQGDANAMTLQSLAHATSPPFAINVSGPEILSVRRTCEQ
                     LGELLGKPPVFAGSEGPDALLSNGQLGHRLFGYPRVAIGQLLAWIADWLKGGGELLGK
                     PTKFQVRDGKF"
     gene            6115..>7248
                     /locus_tag="VFB80_18295"
     CDS             6115..>7248
                     /locus_tag="VFB80_18295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35789.1"
                     /translation="MSDESAKSENQPAGQPLAGENAVVWMLWLTYGAFYFCRTNISAA
                     VPGMTASVGEGGLGLSSDQAGLILASLKITYGIGQFFNGQLAERLSPRVMLALGMFGS
                     ALLNVLFGLGTGFYFLLFVWATNGFFQSLGWTPCVRVAANWVPIVRRGHAIGIIGTGY
                     QITLGLTYLIAGQAAQRLGWQGALYVPAALLALAGVFMLTCLRESPEDRTAALGEKAI
                     TTSQPRLPLGEVLYGTLTNPALWLLGVSLGLLNACRYGFLDWGVKHLMETKQLQVDKA
                     TLQFFVIAIGAVAGSYLAGWATDHLFGGRRAPVICLLLALLAALSFAYDRAAHHSALA
                     TMLLLVVIGFCIYGPQVLLVGTAPADLARRGTSAAAAGFVNFLG"
BASE COUNT         1171 a         2280 c         2384 g         1413 t
ORIGIN      
        1 cggcgaggat gcggcggcgc gattcctgcg aaagctgggc tacgtgattg ttgcccgcgg
       61 gcaccgcgac aacatcggcg agatcgatct ggtcgccgtg gacaagcgga cggtcgtctt
      121 tatcgaggtg aagacccgca ccacgcacga cgccggccat ccagccgacg ccgtggacga
      181 cgccaagcag cgccggctga cgcgcctggc gctatcgtac ctcaagcggc acgacttgct
      241 ggagtgcgca gctcggttcg acgttgtcgc cgtcacgtgg cccgcgggcc gcggcaagcc
      301 cacgatcgag cattttgtca acgcatttga gccggtcggc tttccgggca tgttctctta
      361 gccgcttagc cctggccccc caggttggcg cggcggaaaa aggccgacct ggaagccggg
      421 gttaagcgta gggaatcgga tcgctcgcgc cgttttcagc aaagcctttc agccgcaaga
      481 ggcacgcgtc gcagcgaccg caggcgattc ccgcggcgct gggatcgtag caactgaacg
      541 tgagcgagta atcgacgccg aggtctaggc cgcggcggat gatctgggcc ttggtcaagt
      601 cgatcagtgg cgtgtggatc ttcagcggcc ggccttccac gccggccttc gtggcgaggt
      661 ttgccattcg ctcgaacgcg gcgatgtatt ccggccggca gtcgggatag ccgctgtagt
      721 cgagcgcgtt gacgccgatg aagatgtcgg ccgtgccgct gacctcggca aaagccaggg
      781 cgaacgacag gaagattgtg ttacgggccg ggacgtaagt cagcgggatg ccgccgccga
      841 tctcgcccac cgaggcgtgt ttgggcacgg cgatgtcgtc ggtcagcgcc gagccgccga
      901 actggcgcag gtcgatttcg acgattcgat gtgatacagc gccgagctgc cgcgcgacgc
      961 gctcggcggc cgtcagctcg atcgcatgcc gctggccgta gcgaaagctg agcgcgtggc
     1021 aggcaaagac ttcgctccgc gcgattgcca gcacggtcgc cgaatcgagc ccgccgctga
     1081 gcagtacgac ggcaggacgc tgagcggcca tttatccgag gaccttcttc cgcaagtccg
     1141 tcagcagctt gatcgcgccg ccgatttcgg tcttttgccg ctcgggctcc tgcgcgattt
     1201 cgcgctcgat ggtgagcggg ccgtcgtagc cgatctcctt gagcgtgcgg aggtagtttt
     1261 ccatgcccac gtcgccttgg ccgagcggga cttcctggcc ccattccttg ccgatgttct
     1321 tggcccactt gccgtccttg caatgcacgc tcttcacata gcggccgacc ttcttcagcg
     1381 cggcgatcgg ctcaccggtg ccatagagga tcatgttggc cgggtcgaag ttgatgaaca
     1441 ggttattccg ctcgacatcc tgaatgaact gcaagaggtc gtcagccgtt tcctggccgg
     1501 tctcgaggtg cagcgcctgc ttgttcttgg ccaggtagtc gcacacgtcg cgggcgacgg
     1561 cgacgatctc cttgtaaagc ggcagcgagc ggtcgtgcgg cacaaagccg atgtgcaggc
     1621 cgacgcagga gcagccgagc agcttggcga agtcggcgtt ggccttcagc tccttgatcc
     1681 gctcggcccg cggccccggt ggcacgaggc ctaccgtctt tgcgaccgtc ggaatgtcgg
     1741 cgtagctttc accctcgaac ccgctgaaga cgcaagtcaa cttgatgccg aagccggcga
     1801 gcttcttgag gaaggcttcg gcattggccg gcgtccgcgt cgcggggtgc ggggcgtgca
     1861 gctggatcgt cggcacgcca agatcgcggg cgacctccag cttgacgccg agtcccgcat
     1921 cgacgctggc gaaaactccg atgggccact tttccatggc tagtttccaa gttcaaagtt
     1981 cgaggttagg ccgattgtga atgtagcgcc gccgcagggc gctggcaagc tctcaaagcc
     2041 gcgaccactg gtcgcggtct gagaatgccc gacccgcaga cgaggcagga gcctcggggc
     2101 actgggaacc aggaggaccg cgaccactgg tcgcggcttt gattatgaca atcccgcttg
     2161 gagctgctcg ttctcggact tgaggttgct gccgtcgggc gtttcgtcgc tgttgatgtc
     2221 gtaccattct tccttgaact gcacgaagcc gccttggccg ttcagcgacc tgtcgattgc
     2281 cacgttcgac gtgagcgcga tcaccgcgtc ccccaaggcg acctcggcct tgcaccgcgg
     2341 cgcatcggga gcgttgttgc gaatgcacca ggcaaagtgc tcgatctctt cggtgtagcc
     2401 gcggctgaca gggccgcttt cggcggccgc ccgggcgagc gaggggccct tgccgctggc
     2461 ctgcgtgtcg agcgtggccc ccttgccgtc ttccttcacg ccgaccttgg tcgcggtatc
     2521 cgactctcgg aataacagca cttcctgctc gcggtcgagg atcagggtcc ccttgctgcc
     2581 catcacaatt tcgccgtaac cgccccagcc gttgcccatg atcgacgagt aggtcacgac
     2641 gatccgcttg ttgggatctt cgtcgtagct gaggatgccc ttggtcggat cgggatagtt
     2701 catttcgggg tcgtagtagc cggccttgta gccgggctcg taccccggac cgggaaactc
     2761 gaacgtacaa tagacgtggt cctcggcatc gcggtcgtag ggaaaagtgt gccggccgcc
     2821 gacggcgtgc accgagagcg ggtgggcctt cttcccgtcc ttccgcacgg cgctgacgaa
     2881 gatgctcgag gcgtcgagct ggtggctgcc cagctccgcc atcaacccgc caccagttcg
     2941 ggcaaagagc cgccagcggc agagctcttc gagtgccgtg cgcatgcggc catcgctcaa
     3001 cttaaagtca gagtacccgt atttcgccgc ttcgacgtcc ttgtcgagca cccaggcggc
     3061 ccactgggcg acttgcttct cgagtaagcg tttcttcagt gcgtcctttt cccgcttgag
     3121 cttgtcctgc aggtccttga gcttctccgc cacgaagttg cgtttttcct tctgcttgac
     3181 gccgtcctcg taggtctcga ctgggaacgg caggggctgc tgccagctgt cgttgccggg
     3241 caggttgccg cggtgccact gggcgcggat gtggtgcatt tcgcccaaca ggccccagcg
     3301 gatcaggttg accgcgttgt cgtacagcac gctgtagtgc cgctggtggc cgacggtaag
     3361 gaccttgttc tccttcttgg ccaggcggcc catcacctta cactgggcga cgttgtgggc
     3421 catcagcttt tcggtcagca cgtgcttgcc ggccttgagg gcatccaccg cgacctgggc
     3481 gtgcaggaag agcggcaggg cgatgatcac ggcctcgacg ttcgggtcgt ccagcacctt
     3541 cttgtagtcg ctctcgactt tgatggcgtc cttggcggcc ttctcgtcgg caaagccgta
     3601 cttggcaatc aggccggggc gggcggcgat cgccgcgtcg ccggcccagt cgccgtggaa
     3661 cgcgcggtgc acgttgtacg gccggatgtc gcagatcgac acgaccttga tgtagtcggg
     3721 gttgatagcg ccgatcaaca cgctgccttc atcgccggtg ccgattaccg aaacccgcac
     3781 cggatcgccg atcggcgaat agccaaagta cttgcagccg gtcgtgacac cggcggtcgc
     3841 cgcggctccg acgcccagca ccgtcttgag aaagtcgcgg cgcttccagg cgtcggcggc
     3901 cggcgactgg tcgaatgagc tcacggcgga gtaatagttc tccttgccga cttcgcgttc
     3961 ttcgggggtg aggttgttca tttcgcggct ccttgtttct tcggcgggca gcagagggcc
     4021 ttcatgccgc cgaggagata atcgattccc aggaattttc ccgcgccgac ggctgccagg
     4081 accagcaggg cgagcatttc tacggcttga tagaagaccg ggatcgcccc gggcgagccg
     4141 ggccactgcg tggtgcaaac catcgccaga aacgctgccc cggccagcgc cgccacgcgc
     4201 gtgaacagtc ccagaatcag gcagacgccg atcgtcaggt cgaaataggg cacgagccag
     4261 tcgagcgtga ccgtatcaaa gctgcggcgg ccgggcttgc cgagggacag ccggccgtga
     4321 cgacgccact ggtcgtcggt ggcgatggcg ttgaggtcgt tttcgaaatc gcgccacagg
     4381 ccctcgatcg tgggcaagag ctccccgcgc atctttagca actcggcgtt gatcttggcg
     4441 tcgtgaactt gcagtgacgc gagatttcgc gtgggatcac ccgcgttggc gtcgcggcgg
     4501 tccagccgct gataatactc gtcgatcttg tcctgattga cgccgagaaa gtcgttcagc
     4561 cgctgcgtgt aatccttcag gacgtcagct gcctctttcg tttgcgcctc gtcgaagcgg
     4621 taatgcgtca cgatgctgct gcggaactgg tcccattggg ccttggcgat gtcgagcgac
     4681 agccgaaagc ggccgtccgc gtcccaaacc attcccttgt acacgtcggc cagcggccct
     4741 ttggcagcac cgaagaacgg cccggaaaat ggcttggggt cggccagctt atcgacccct
     4801 tcggccagaa aatgcacgcc gattcccact cgcagcagga ccacggctgc ggcggcgagc
     4861 cagccaattc gatacgatga gtcggtcacc gtcgctccgg agaggcagaa gtctcggggt
     4921 aaacttagtc ttcacattat ggctgcgaag tgccattccg ccaacgggcg gcggggccga
     4981 gttcaaagtt aaagttcaaa gttggccaat atggctggac tgccggatcg tgttgccagt
     5041 ggtgagcaac tcgacgattt gttgagtgag ccgacggctg gcgtcatcca gactctgggc
     5101 aagttggatg gcgacctgtt ggtgctgggc gtgggcggca aaatggggcc gacgctggcg
     5161 cggatggccg tgcgggcgtc ggagcaggcg agcgtgaagc ggcgcgtcat cggcgtcgct
     5221 cgattcagca atcgcgagct accggcgtgg ctcgcgcggc acggggtcga gccgctgacg
     5281 gcggatctgc tcgacccgcg gcaggtggcc cagcttccgg acgccgcgaa cgtgatcgtg
     5341 atgacggcgc tgaagtttgg tgcgagcggc cggccgggcg atacctgggc cgtgaattgc
     5401 tggctgccag ccaacatctg ccagcgattc gccggcagcc gcatcgccgc gttttcgacc
     5461 ggcaacgtct atccgctgac gccgatcgcc cgtggtggct ccctcgaaac cgatccgctt
     5521 gggcccgtcg gcgaatatgc cgccagctgc gtcggccgcg agcggatgta cgactatttc
     5581 agccgcttgg ccggcacgaa gatttcaatt ctgcggctaa actacgcctg cgagctgcgg
     5641 tacggcgtgc tggtcgatct ggcgcagcag atactggcga gagaaccgat cgacctgacg
     5701 atgggcgcgg tgaacgttat ctggcagggc gacgcgaacg ccatgacgct tcagtcgctg
     5761 gcccacgcca cgtcgccccc ctttgccatc aacgtcagcg gcccggagat tctctctgtc
     5821 cgccgcacct gcgagcagct gggcgaattg ctcggcaagc cgcccgtctt tgcgggcagc
     5881 gaaggccccg acgcgctgct gtccaacgga cagcttggcc atcggctgtt cggctatccg
     5941 cgtgtggcga tcggacagct gctcgcctgg atcgccgact ggctgaaggg cggcggcgaa
     6001 ctgctgggta agccgacgaa gttccaggtg cgggatggca agttctagca taagtagcgt
     6061 cggcctcccg ccgccgttag actcgggcgg cacttgggtt cccgcatggc atctttgagc
     6121 gacgaatcag caaagtccga gaaccagccg gccggccagc cgctggccgg cgaaaacgcc
     6181 gtcgtctgga tgctctggct gacctacggg gcgttctatt tctgccgcac caacatctcg
     6241 gcagcagttc cgggcatgac ggcgtcggtc ggcgaaggag gactcggcct ctcgagtgac
     6301 caggccggct tgattctggc gtcactgaag atcacgtacg gcatcgggca attcttcaat
     6361 ggccaactag ccgaacggct gtcgccgcgg gtgatgctgg cgctaggcat gttcggctcg
     6421 gcgctgctca acgtgctgtt cggtctgggc acggggtttt acttcctgct gttcgtctgg
     6481 gcgacgaacg gattcttcca gtcgctcggc tggacgccct gcgtgcgcgt cgccgccaac
     6541 tgggtaccga tcgtccgccg tggtcacgca atcggcatca tcggcaccgg ctatcagatc
     6601 acgctggggc tcacctacct catcgccggc caggcggccc agcggctggg ctggcaaggt
     6661 gcgctatacg ttcccgccgc gctcttggcc ttggcgggcg tcttcatgct gacgtgcctg
     6721 cgggaatcgc ccgaagatcg cacggccgcg ctcggcgaaa aggcaatcac gacaagccag
     6781 ccgcgattgc cgctgggcga agtcctgtat ggcacgctga ccaatcccgc cttgtggctg
     6841 ctgggggtct cgctgggcct gctcaacgcc tgccgctacg gctttctgga ttggggcgtg
     6901 aagcacctga tggaaacgaa gcagctgcaa gtcgataaag cgacgctgca gttcttcgtg
     6961 attgccatcg gagccgtggc gggctcctat ctggcgggct gggcgacgga ccacctcttc
     7021 ggcggccgcc gcgcgccggt aatctgcctt ttgctggccc tgttggcagc actttcgttc
     7081 gcatacgacc gggcggccca ccacagcgcg ctggctacga tgctgctgct cgtggtgatc
     7141 ggcttttgca tttatggccc gcaggtgctg ctggtcggca cggcccccgc cgacctggcc
     7201 cgccgcggga ccagcgctgc agccgcggga ttcgtgaatt tccttggc
//
LOCUS       DASRJB010000688         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3865676, whole genome shotgun sequence.
ACCESSION   DASRJB010000688 DASRJB010000000
VERSION     DASRJB010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865676"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..487)
                     /locus_tag="VFB80_18300"
     CDS             complement(227..487)
                     /locus_tag="VFB80_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35790.1"
                     /translation="MIDSTEYWPALHRLSETSDRQGMGTDERLDAIVKQFLQMPPQIR
                     RQLIADVLRLSLELPDVYAAVTAAANQAEEQDLRRLRRGETG"
     gene            complement(511..768)
                     /locus_tag="VFB80_18305"
     CDS             complement(511..768)
                     /locus_tag="VFB80_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35791.1"
                     /translation="MISANEFWLNLHRLMEAYEAEGLTSDERAANIVAEFQLLPLTTQ
                     RQLLADLLKLSTRCPDLYPMIVAAANEAEAEAASQQELDAG"
     gene            complement(863..1459)
                     /locus_tag="VFB80_18310"
     CDS             complement(863..1459)
                     /locus_tag="VFB80_18310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN35792.1"
                     /translation="MIRGIVFDMDGTLVDSRLDFDAMRREMELPPQMPILEAVTRLPP
                     PHADRCRAILDRHELAGVARAALLPGVAELFAELRSREIRLAIATRNSRANTATTLEK
                     LSVQVDFALTRDDGPVKPDPWAAVEACRRWELPPQCVAVIGDYRFDVECGRAAGCRTV
                     LLTHPHDPAVYPNDERADLLLLSLAEYQRLLVWLNVQQ"
     gene            complement(1456..2397)
                     /locus_tag="VFB80_18315"
     CDS             complement(1456..2397)
                     /locus_tag="VFB80_18315"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HZN35793.1"
                     /translation="MSQADCSLRDTAAALGRGWNAFFHAPCDARVCALVRIAFACVVL
                     ANFAALWPDRERWFTDAGVLTAETSQRIASPYGWSLLWLLPSTTEVVNVCLWIAVANT
                     VLLLIGLLPRLNALCVLVWLYSFQTRNVQIIDGEDTVMRMTAFFLIWMPSGQCWSLHA
                     LLRRWLGFPPLGCEAPGWSLRLLQIQMASILLSTALLKLAGDEWIDGTALYYVSRLDD
                     FWGRFPVPAWLFDTPWTVALMTWAVLVVELTVPVLIWFKETRRWCLLVTVLFHLANEW
                     TMHLFLFHWIMLVGWLSFLTAGDLAWFTGKSQRGAAA"
     gene            complement(2394..2978)
                     /locus_tag="VFB80_18320"
     CDS             complement(2394..2978)
                     /locus_tag="VFB80_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35794.1"
                     /translation="MSAAARSSTETTPAPPRLWSRLRLIAVNAFIAAFLVLLAIDVMP
                     QSPLALRLKLQRVLAPLGIAQGPWNLFAPGPDRTNIRLRAEITYRDGQRQEWSAPTWR
                     EQSAAQMWWGHRRHAWYDHLVAQEAGPIWEQWCRHLAREQRPDLADADRGAVVRVIYQ
                     LGPVAPADLKPWRSIGEPPQYEPHELVLTIERLE"
     gene            complement(3031..3252)
                     /locus_tag="VFB80_18325"
     CDS             complement(3031..3252)
                     /locus_tag="VFB80_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35795.1"
                     /translation="MPSTVSQPPFQFDPTASQSGSRVYYLLWRPRSNQPWQSEEHFSR
                     IEAHNKYFSLMQRGYEVYLERRNRLASPA"
     gene            3390..>4574
                     /locus_tag="VFB80_18330"
     CDS             3390..>4574
                     /locus_tag="VFB80_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZN35796.1"
                     /translation="MKTLIQGATVVLPTGCVQTDILLDRGRIAAIDAARHTAADEVVA
                     ARGLHLLPGVIDDQVHFREPGLTHKEDLYTATRACAKGGVTTFLEMPNTNPTTTTCQR
                     LDEKLALAASKCLVNFGFYIGATPHNLSELNSAKRTPGIKIFIGSSTGDLLVDDQAAL
                     EAIFAETTLPICAHCEDEATIRANAARLAGTTNVADHSRIRDHAAAIVATRRALDLAF
                     RHRHRFHVLHVSTGDEADLVADHRGLITAEVCPHHLFFSTDDYARLGALVQMNPSLKT
                     PDDNRRLWQALLDGRIQVIATDHAPHTLEEKAKPYSPSHGGSPSGLPAVENSLALLLN
                     EASRGRCTLEQVAHWMCEAPARVWDLVGKGRIEIGYDADLVLVDLNKTATILNEQQET
                     KSR"
BASE COUNT          855 a         1504 c         1484 g          731 t
ORIGIN      
        1 agagcgtatc ggcctcgttc gccgccgcgg tcacgagcgg gtggaggtcc gggcagttcg
       61 cggcgatgtt ggacaggtcg gccagctgct tgcgctgaac gacagtcggc agctcccgga
      121 attgggcgac gatgtttgcc agccgctcgt cgttcgtcgg cccctcggcg tccgcgagtt
      181 gattgaccgc atgccagaac tatgggggcg aacgtcgtgg attccgctat cccgtttccc
      241 cccggcgcag ccggcgcaaa tcctgttcct cggcctgatt cgcggcagcg gtcacggcgg
      301 cgtagacgtc gggcaattcg agcgacagcc gcagcacgtc ggcaatcagc tgccggcgaa
      361 tctggggagg catttggagg aactgcttga cgattgcgtc gaggcgctcg tcggtcccca
      421 tgccctggcg gtcgctggtc tccgacagcc ggtggagggc gggccagtat tcggtcgagt
      481 cgatcatgtc gtcaccccag ggctgctgcg tcagcctgca tcgagttcct gctggctggc
      541 ggcctccgct tcagcctcat tggccgccgc cacgatcatc ggatacaagt ccgggcacct
      601 ggtcgagagc ttgagcaaat cggccagcag ctgccgctgg gtcgtcaggg gcagcagctg
      661 gaactcggca acgatgttgg cggcccgctc gtcgctggtc aacccttcgg cctcataggc
      721 ttccatgagc cggtgcagat taagccagaa ttcgttggcg gagatcatcg tgttccccgg
      781 ggaatagccg cgcggcagca gcgcgcccag gtccccatgc gcagtttgac gctcacgcgg
      841 gataatacaa gcaggcagag ctttattgct gtacgttcag ccagaccagc aggcgctggt
      901 actcggcgag cgacagcagc agcaggtccg cgcgctcgtc gttgggatag acggccggat
      961 cgtgcggatg cgtcagcaat accgtgcggc agccggcggc gcggccgcat tcgacgtcga
     1021 agcggtagtc gccgatcacg gcgacgcact gggggggcaa ttcccaacgg cggcaggctt
     1081 ccaccgcggc ccacggatcg ggcttcaccg gcccgtcgtc gcgggtcagt gcgaaatcaa
     1141 cctgcacgct cagcttctcg agcgtcgtgg ccgtgttcgc ccggctgttg cgggtggcga
     1201 ttgccaggcg aatctcgcgg gaccgcagct cggcaaacag ctcggcgacg ccaggcaaga
     1261 gtgccgctcg cgcgactccg gcgagctcgt ggcgatcgag aatcgcccgg cagcgatcgg
     1321 cgtgcggcgg aggcagccgc gtgaccgcct cgaggatcgg catttgcggt ggcaactcca
     1381 tctcgcggcg catcgcgtcg aagtcgagcc gcgagtcgac gagcgtccca tccatgtcga
     1441 acacgatgcc gcgaatcacg ctgctgctcc tcgctgcgac tttcccgtga accaagccaa
     1501 atcgcctgcc gtgagaaagg acagccagcc gacgagcatg atccagtgga acaggaacag
     1561 gtgcatcgtc cattcgttgg cgaggtgaaa cagcaccgtc accaacaggc accacctccg
     1621 cgtctccttg aaccagatca gcaccggcac ggtcaattcc acgaccagca ccgcccaggt
     1681 catgagggcc accgtccacg gcgtgtcgaa cagccacgcc ggcacgggaa agcggcccca
     1741 gaagtcgtcc agccgggaga catagtacag cgccgtgccg tcgatccact cgtcgccggc
     1801 cagtttcagg agggccgtgg agaggaggat ggacgccatt tgaatctgca gcaggcgtaa
     1861 gctccagccc ggcgcttcac aacccaacgg tgggaatccg agccagcggc gcagtagcgc
     1921 atggagcgac cagcactgcc cgctgggcat ccagatcaga aaaaacgccg tcatcctcat
     1981 cacggtgtct tcgccgtcga tgatctgcac attccgcgtt tgaaacgaat aaagccagac
     2041 cagcacgcac aaggcgttca ggcgcggcag cagcccgatg agcagcagca ccgtgttggc
     2101 gaccgcgatc cacagacaaa cgttgacgac ctcggtcgtg ctgggcaaca gccagagcag
     2161 cgaccagccg taggggctgg cgatgcgctg cgaagtttcg gcagtcagca ctccggcgtc
     2221 ggtaaaccac cgttcccggt caggccacag cgcggcaaaa tttgccagca ccacgcaagc
     2281 gaaggcgatt cgcaccaggg cgcagacccg cgcgtcacac ggcgcatgga aaaacgcatt
     2341 ccagccgcgc cccagcgcgg ccgccgtatc gcggaggctg cagtcggcct ggctcattcg
     2401 agtctctcga tcgtcagcac cagctcgtgc ggttcatatt gtggcggttc gccgatgctc
     2461 cgccacggct tcaagtcggc cggcgcgacg ggaccgagct gataaatgac gcggacgacg
     2521 gccccgcgat cggcatccgc caggtcgggc cgctgctcgc gggccagatg ccggcaccac
     2581 tgttcccaaa tcggtcccgc ctcctgggcc accaggtggt cgtaccaggc gtgccgccgg
     2641 tggccccacc acatctgggc agccgattgc tcgcgccaag tcggcgcgga ccactcctgc
     2701 cgctggccgt cgcggtacgt gatctcggcc cgcaagcgaa tattcgtccg atcggggcca
     2761 ggcgcaaaca ggttccacgg accctgcgcg atgcccagcg gcgccagcac gcgctgcagc
     2821 ttgagccgca aggccagcgg cgactgcggc atgacgtcga tcgcgagcag gaccagaaac
     2881 gccgcgatga acgcgttgac ggcaatcagc cgcaggcgcg accacagccg cggcggagcg
     2941 ggcgttgttt cagtcgatga gcgagcggcg gcggacatac cgctcaagtt taggtccgtc
     3001 cgccgcggcg gcgtatggca attgtgccgg ctacgcgggc gaggccaggc gatttcgccg
     3061 ctccaggtaa acttcgtatc cccgctgcat cagcgaaaaa tacttgttgt gggcctcgat
     3121 ccggctgaag tgctcttcgc tctgccacgg ctggttcgac cgcgggcgcc acagcagata
     3181 gtagacgcgc gagcctgact gcgaggcggt aggatcgaat tggaagggag gctgactaac
     3241 cgtcgaaggc atggaacgtc tcgggcatgg gtcgtgccgc tgcgccagcg agcggccact
     3301 tgctgctaag cgcacatcgg gcgaacttgt tacaactaaa tcccctaggg atatcgctgc
     3361 cggccaactc caacccgaca tccactgccg tgaagacgct gattcagggc gcaactgtcg
     3421 ttctgccaac gggttgcgtc caaacggaca tcttgctgga tcgcggccgg attgccgcca
     3481 tcgacgcggc caggcacacg gcagccgacg aggttgttgc ggcccgcgga ctccacctct
     3541 tgccgggcgt catcgacgat caggtccatt tccgggaacc ggggctgacg cacaaggaag
     3601 acctttacac cgccacgcgg gcgtgtgcga aaggtggcgt gacgacgttc ctggagatgc
     3661 ccaacaccaa tccgacgacc acgacttgcc agcgactcga tgagaaattg gcactggcgg
     3721 ccagcaagtg cctcgtcaac ttcgggttct acatcggcgc tacgccgcac aacttgagcg
     3781 agctgaacag cgcgaagcgg acgccgggaa tcaagatctt catcggctcc agcacgggcg
     3841 atctgctcgt cgatgaccag gcggcgctgg aggcgatctt tgccgagacg acgctgccga
     3901 tctgcgccca ttgcgaggac gaagcgacga tcagggctaa cgccgcgcgg ctggccggga
     3961 cgaccaacgt cgccgaccac agccgcattc gcgatcacgc ggccgcgatc gttgcgacgc
     4021 ggcgggcgct ggacctggcg tttcggcatc ggcatcggtt ccacgtgctg catgtctcga
     4081 cgggtgatga ggccgatctg gtggccgacc accgcggcct gatcacggcg gaggtctgcc
     4141 cgcaccactt gttctttagc accgacgact acgcccgcct gggcgcgctg gtgcagatga
     4201 atccgtcgct gaagaccccc gacgacaacc gccgcctgtg gcaagcgctc ctggacgggc
     4261 ggattcaagt catcgcgacc gatcatgccc ctcacacgct cgaggaaaaa gccaaaccgt
     4321 attcgcccag ccacggcggc tcgccgtcgg gcttgccggc ggtcgagaac tcgctggccc
     4381 tcctgctcaa cgaagccagc cgcgggcgct gcacgctgga gcaagttgct cactggatgt
     4441 gcgaagcgcc ggccagggtc tgggacctcg tcggcaaggg gcggatcgaa atcggctacg
     4501 acgccgacct ggtgctcgtc gatctaaaca agaccgccac cattctgaac gagcagcagg
     4561 aaacgaaatc ccgc
//
LOCUS       DASRJB010000689         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2166921, whole genome shotgun sequence.
ACCESSION   DASRJB010000689 DASRJB010000000
VERSION     DASRJB010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166921"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="gcvPB"
                     /locus_tag="VFB80_18335"
     CDS             <1..961
                     /gene="gcvPB"
                     /locus_tag="VFB80_18335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZN35797.1"
                     /translation="VLAPDSAHGTNPASARMAGFEFLTVKSREGGIVDLEDLQSKLDD
                     QIAVFMITNPSTLGLFDQQVPRIAQLVHERGGLIYLDGANMNAILGITRPGDFGSDMM
                     HYNPHKTFSGPHGGGGPGAGPICVAEKLGPFLPTPVVEHRDGRYVLTFDRPLSIGRVR
                     SFFGNVGVLVRAYCYIRTHGPDGLKAVAENAVLNANYLLSRVKHILPVPQGDRCMHEF
                     VATAAPLKKKNGASAMDLAKRLLDFGFHAPTVYFPLTVPEAMMIEPTETESKETLDAF
                     AEALFRITEESPDLLHEAPHSTLISRPDEVRAARQPMMKWKPL"
     gene            958..1713
                     /locus_tag="VFB80_18340"
     CDS             958..1713
                     /locus_tag="VFB80_18340"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HZN35798.1"
                     /translation="MKPLRLLIDPPAGGAWNMAVDEALLESAAAEGIATLRFYQWAPA
                     TLSLGYFQAAADRRQHAASQDCPLVRRASGGGAILHDCELTYSIALPQDAARSSATAL
                     YERCHESLVEALAQLGASAALYRLPVGCDAILRQNAAEAFLCFQRRTCGDIVLGNWKI
                     AGSAQRRRRGAVLQHGSILLGRSSFAPELPGIRELFGQTFSADSVIDAWLPRLAARLA
                     LAPQRGELGELQARRARELVAERFGADRFTQQR"
     gene            2001..2678
                     /locus_tag="VFB80_18345"
     CDS             2001..2678
                     /locus_tag="VFB80_18345"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN35799.1"
                     /translation="MQVRLKIVQGANVGKEIKIPSPKCVIGRGDDCHLRPQVDAISRR
                     HCVVITTENEAVVRDLNSRNGTFVNNERVAEETVLLTGDVLRVGPLEFEVIVEQAPGK
                     PKRPKVSDIKEAVARTAEGSSVGATTELEDVSAWLEEADTEEKQRRYTDPETRQFRLD
                     DTATPPEEGTTGPTTETKALKPDQKEGKAGKDKKEPGKLPPRPSTSSKDSREAAADML
                     KKFFNRR"
BASE COUNT          554 a          985 c          967 g          481 t
ORIGIN      
        1 ggtcctggct cccgacagcg cccacggcac gaatccggcc agcgcccgga tggccggctt
       61 cgagtttctc accgtgaaaa gccgcgaggg cggcattgtc gatctggaag acctgcagag
      121 caagctcgac gaccagatcg ccgtgttcat gatcaccaac ccgagcacgc tgggcctgtt
      181 cgaccagcag gtgccgcgaa tcgcccaact cgtgcacgag cggggcgggc tgatctatct
      241 cgacggcgcg aacatgaacg cgattctcgg catcacgcgg ccgggcgatt tcggttcgga
      301 catgatgcac tacaacccgc acaagacctt tagcggcccg cacggcggcg gtggccccgg
      361 cgcggggccg atctgcgtcg ccgaaaagct cggcccgttt ctgccaacgc cggtcgtcga
      421 gcaccgcgac ggccgctacg tgctgacgtt tgaccggccg ctttccatcg gccgcgtccg
      481 cagcttcttc ggcaacgtcg gcgtgctggt gcgggcttac tgctacatcc gcacacacgg
      541 cccggacgga ctgaaggccg tggccgaaaa cgccgtgctc aacgccaact acctgctcag
      601 ccgcgtgaag cacatcctcc cggtcccaca gggcgaccgc tgcatgcacg agttcgtcgc
      661 caccgccgct ccgctgaaaa agaagaacgg cgccagcgcg atggacctcg ccaagcggct
      721 gctggacttc ggctttcacg cgccgaccgt ctattttccg ctgaccgtgc cggaagcgat
      781 gatgatcgaa ccgaccgaaa ccgagagcaa ggaaacgctc gacgccttcg ccgaagcgct
      841 gttccgcatc accgaggaat cgcccgacct gctgcacgaa gcgccccaca gcacgctcat
      901 cagccggccc gacgaagtcc gcgcggcccg gcagccgatg atgaagtgga agcccctgtg
      961 aagccgctcc gcctgctgat cgatccgccg gccggcggcg cgtggaacat ggcggtcgat
     1021 gaggcgctcc tggagtccgc ggcggccgag ggaatcgcca cgctgcgatt ctatcagtgg
     1081 gcgccggcga cgctgtcgct gggatatttt caagcggcag ccgatcgccg gcagcacgcg
     1141 gccagccagg attgcccgct agtgcggcgg gccagcggcg gcggggccat cctacacgac
     1201 tgcgagctga cctacagcat cgcgctgccg caggacgcag ctcgatcgtc cgcaaccgca
     1261 ctctacgagc ggtgccacga gtcgctggtt gaggcgctgg cgcaactggg ggcgagcgcg
     1321 gccctctacc ggctgcctgt cggctgcgat gcgatactgc ggcaaaatgc cgcagaagcg
     1381 tttctgtgct ttcagcggcg gacttgcggc gacatcgtgc tggggaattg gaaaatcgcc
     1441 ggcagtgctc agcggcgtcg ccgtggtgct gtgctccagc acggcagcat tctcctcggc
     1501 cgctcgtcct tcgcaccgga gctcccgggc atccgggagc ttttcggcca aaccttctcg
     1561 gccgatagcg tgatcgacgc ctggttgcct cggctggccg cgcggctggc gctagcgccg
     1621 cagcgcggcg agctgggcga gcttcaagcg cgacgggccc gcgagctggt cgccgagcgt
     1681 tttggggccg accgcttcac ccaacagagg tagtcgcaaa atcgctgcaa tttcctacca
     1741 atcggagctc cgctagttgc aattcgcatt ggccctgagc gataataagg ctgcgtcaat
     1801 ggtctgaaag tttgcggcgc gcttgcgggt cctcatccat ccttgtctgg tttccgtgcg
     1861 cggcgctgac gattgacggc agcgggttgt tcagacactt ttcgtggagg cccaagccga
     1921 gaccggcccg actctccgcc atcgttgcgt tcatcattcg cagtttagcg ccgggtctca
     1981 aatcacttgg gagttgcaag atgcaagtgc gactgaaaat cgtgcagggt gcgaatgtcg
     2041 gcaaggaaat caagatcccg tcgccgaagt gcgtgatcgg ccgcggcgac gactgccacc
     2101 tccgcccgca ggttgacgcg atcagccggc ggcactgcgt agtcatcacc accgagaacg
     2161 aagcggtggt ccgcgacctg aacagtcgca acgggacctt cgtcaacaac gagcgggtcg
     2221 ccgaagagac ggtgctgctc acgggcgatg tactccgcgt cggacctctc gagttcgagg
     2281 tcatcgtcga gcaagcgccc ggcaagccca aacgccccaa ggtctccgac atcaaggagg
     2341 cggtggcccg cacggccgaa ggcagcagcg tcggcgcgac caccgaattg gaagacgtca
     2401 gcgcctggct ggaagaagcc gacaccgaag aaaagcagcg ccgctacacc gacccggaaa
     2461 cgcggcagtt ccggctggac gatacggcca cgccgcccga agaggggacg accgggccaa
     2521 cgacggagac caaggcgctc aagcccgacc agaaggaagg caaggccggc aaggacaaga
     2581 aagaacccgg caagctgccc ccacggccct cgaccagcag caaggactcg cgcgaggcgg
     2641 ccgcggacat gctcaagaag ttcttcaatc gccgctaagt cgtttcgcgg catccggtta
     2701 ggcgacaaag ccgcggccac tggtcgcggc tttggttgtc aatcgcaaga tttcctgccc
     2761 ctaagtagaa tacacggcag aatcgctgcg ggcggctcct tgctggccgc cttttggtga
     2821 aaccagcccg cgtgtcggct ggtactaccc tcgtgccggc tgccaatgag cggtttgggg
     2881 cttatcgacc tgaagcagct cgtaggccgc tgcctggcgg gcgaccaggg ggcgatgctc
     2941 cagctggtcg agaggtaccg gggccaggtg ttcggtctgt gctatcg
//
LOCUS       DASRJB010000690         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3866072, whole genome shotgun sequence.
ACCESSION   DASRJB010000690 DASRJB010000000
VERSION     DASRJB010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866072"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB80_18350"
     CDS             complement(<1..574)
                     /locus_tag="VFB80_18350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN35800.1"
                     /translation="MFHYDGGLRITSIDLGVDVCRRQPRGFVSHAHADHMARHELAFC
                     TPATAALYRHRFGPRPVREMPLGEPLALGELTLTALPAGHVLGSAMLHIRGPAGTLLY
                     TGDFKLGASATAEPAAPPRADVLIMESTFGHPKYRLPPREEVIGELIAIVRRILEVDR
                     TPVVQAYVLGKAQEVTRILTSAGFRVVQHPL"
     gene            complement(584..1465)
                     /locus_tag="VFB80_18355"
     CDS             complement(584..1465)
                     /locus_tag="VFB80_18355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN35801.1"
                     /translation="MYKNLNPSAIGIFGRQSELVEISLTHRFKGLEIDITEVTKRAQT
                     TSVAQACRYLCSAPLTIAGFELPIRWAGDEKEFKNDLAQVPLLLEVAGALNADRCTTT
                     IRPTCDQRPFHENFKFHVARLSQLAEALAPGNVKLALNFLAAPSDRADGGFEFIHQAD
                     AALTLVNSIGKDNVGLLLDAWSWTVGGGEFEKLRTLRPEQIVSVRLSDIPAGVDLVSI
                     TNEQRLFPGEGGTIDSLALLSLLEEIGFDGPVAAAQWPDASKGQKREETVKKASAALD
                     AALAGLESAKPLEAAGQ"
     gene            1685..2527
                     /locus_tag="VFB80_18360"
     CDS             1685..2527
                     /locus_tag="VFB80_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZN35802.1"
                     /translation="MFVDGHGSVVHPEAMQFTLWQVLVLAVVQGVTEFLPISSDGHLA
                     VIKPLLFGGDTAPSSLDLTIALHLGTLGSILVFYWRRILRLLGEDRRVIGLLIVGTLP
                     LIPLVLALKVLLGDDFEKALENPLLAGCMLPASGLALLWSLRQPPGTIEYRELPWWKS
                     LLIGIAQATAILPGLSRSGTTIASGIGLGMSRSSAASFSFLLAIPALAGAGAYKGLSL
                     LKDGEPLSAPASYLVLGAVVSFVVGLAALRCLDALLQRGQLHWFGWYCIALGAAVVVW
                     QLAT"
     gene            complement(2597..3622)
                     /locus_tag="VFB80_18365"
     CDS             complement(2597..3622)
                     /locus_tag="VFB80_18365"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZN35803.1"
                     /translation="MPPLASLPRVAALLACTAAWPAAAADVAELDPAAVLAQHGLARV
                     DRVWVLADELRLRRDLAELPKRRERLLAVEHDLDQRIEKNRQRWQEAQPAIAALRQSL
                     ARLGSEDPQRQLLAQQIDALAAAAAEPTKIGAKSEVRSRVLVWIDERNSLASALASIR
                     GTIGRLGELYEQLAQTPGVAEALRQIGDKNRLGPQRSLAADRERLSEYEKLTYTPWVP
                     IFFQGAQLRLTALIDDLTPVTFTWIDSGGPGIILTRTAAEAAGLKIPAAAPQEAVAIS
                     PQKRTTAWRINVARLRLGKCVLQDASVLVLPPEAEDWGCQLSREALSGHALRLEPERL
                     RLSIDAG"
BASE COUNT          574 a         1263 c         1232 g          673 t
ORIGIN      
        1 ccagcggatg ctgcacgact cggaaaccgg cactcgtcag aatccgcgtc acttcctgtg
       61 ccttgccaag cacgtaagcc tgtacgaccg gcgtgcgatc gacttccaaa atccgccgca
      121 cgatcgcgat caactcgccg atcacctctt cgcgcggcgg caggcggtac ttcggatgac
      181 cgaacgtgct ctccatgatg agcacgtcgg ctctcggcgg agcggcaggc tccgccgtcg
      241 cagacgcccc cagcttgaaa tcccctgtgt acagcagcgt gcccgccggg ccgcggatgt
      301 gcagcatcgc cgagcccagc acgtgccccg ccggcagcgc ggtcagcgtc agctcgccca
      361 gcgccagcgg ctcgcccagc ggcatctccc gcaccggccg cgggccaaag cggtgccgat
      421 acagcgccgc agtggccggc gtgcaaaacg ccaactcgtg ccgcgccatg tgatcggcgt
      481 gagcgtggga gacgaaccct cgcggctgcc gacggcaaac atcgacgccc aggtcgatgc
      541 tcgtgattcg caggccgccg tcgtagtgga acatgccact actttactgc cccgcggcct
      601 ccagcggttt ggcgctttcc aggccggcca aggcggcgtc gagcgctgcc gaggccttct
      661 tgaccgtctc ctcgcgcttc tggcctttcg aggcgtcggg ccactgcgcg gcggcgacgg
      721 gaccatcgaa gccgatctct tcgagcagcg acagcagggc gagcgagtcg attgtgcccc
      781 cttcgccggg aaacagccgc tgctcgttgg tgatcgaaac gagatccacg ccggcgggaa
      841 tgtcggacag ccggacgctc acgatctgct ccggccgcag cgtgcggagc ttctcgaatt
      901 cgccgccgcc aacggtccag ctccaggcgt cgagcaagag gcccacattg tccttgccga
      961 tcgagttgac cagcgtcagt gcggcgtcgg cctggtggat aaactcgaat ccgccgtcgg
     1021 cccggtcgct gggagcggcc aggaagttca gagccagttt cacattgccg ggtgcgaggg
     1081 cctcggccag ctggctcagc cgcgcgacgt gaaacttgaa gttctcgtgg aagggccgct
     1141 ggtcgcaggt cggccggatc gtcgtcgtgc agcggtcggc gttcagggcc ccggcgactt
     1201 ccagcaacag ggggacctgg gccaggtcgt ttttgaactc cttttcgtcg cccgcccagc
     1261 gaatgggcag ctcgaagccg gcaattgtca gcggggcact gcacaaatag cggcaagcct
     1321 gggcgacgct ggtcgtctgg gcccgcttcg tgacctccgt gatgtcaatt tcgagcccct
     1381 taaaccggtg ggtgagtgag atctccacca actcactttg ccggccgaaa atgccgatgg
     1441 cggaaggatt taggttcttg tacatgcgac tccctttctg ttccgtaaca gttctcggtc
     1501 ccacagcgtt ccgttggggg aatcagccgc cgacagcgtg ctcgccgccg gccattcccc
     1561 gggtgataag acctcccatt atcccgggga cggtagatca cacaacccgg gcaccctcca
     1621 gaagggtgat ccggcaaaag attaacaccc tttactggac tcccgccatc ctggcaagcc
     1681 gggaatgttc gttgacggcc acggcagcgt cgtccaccct gaggcgatgc aattcacgct
     1741 gtggcaagtg ctggtactgg cggttgtcca gggcgtgacc gaattcctcc cgatcagctc
     1801 cgacggccac ctggccgtga tcaagccgct gctcttcggc ggcgatacag cgccgtcctc
     1861 gctggacctt acgatcgcac tgcacctggg cacgctgggc tcgatcctgg tcttctactg
     1921 gcggcgaatt ctgcgcctcc ttggcgaaga ccggcgcgtc attggcctgc tcattgtggg
     1981 cacgctcccg ttgatcccat tggtgctggc gctgaaggtc ctgctgggcg atgacttcga
     2041 gaaagccctg gagaatccgc tgctggccgg ttgcatgctg cccgccagcg ggctggcgct
     2101 gctgtggtcg ctccggcagc cgccggggac gatcgaatac cgcgagctgc cgtggtggaa
     2161 gtcgctgctg atcggcatcg ctcaagccac agcgattttg cccgggctat cgcggagcgg
     2221 aacgacgatc gcctccggca tcgggctcgg catgtcccgc tcgtccgcgg cttcgttttc
     2281 gttcctgctg gcgattccgg cgctggccgg cgctggcgcc tacaagggac tctcgctgct
     2341 caaggacggc gagccgctat cggcgccggc cagctacctc gtcctcggcg cggtcgtttc
     2401 gtttgtggtc ggcctggccg cgctgcgttg tctcgacgcg cttctgcaac gcggccaact
     2461 gcactggttc ggctggtatt gcatcgcact cggcgcggcg gttgtcgtct ggcaactggc
     2521 cacctaggcc cgcagtcttc gccggattcg gcgcgcgaag cgatccccga aagtgggtcc
     2581 ggggttttgc caggctttag ccggcgtcga tcgatagccg caggcgctcg ggctccaggc
     2641 ggagcgcgtg gccgctgagg gcctcgcggc tgagctggca gccccagtct tcggcctccg
     2701 ggggcaggac cagcaccgag gcatcctgaa gaacacactt gcccagccgc agccgggcca
     2761 cgttgatccg ccaggcggtg gtccgtttct gtggcgagat cgcgacggcc tcttgcggcg
     2821 cggcagcggg gattttcagg cccgcggctt ccgctgcggt ccgagtgaga atgatgcctg
     2881 gcccgccgct gtcgatccag gtaaaggtca ccggcgtgag gtcgtcgatc agagccgtca
     2941 atcgcagctg tgcaccctga aagaagatcg gcacccaggg ggtgtaggtc agcttctcgt
     3001 attcgcttag ccgctcgcgg tcggcggcca ggctgcgctg cggaccgagg cggtttttgt
     3061 cgccaatctg gcggagggcc tcggcgaccc ccggcgtctg cgcaagctgc tcgtagagct
     3121 cgcccagccg gccgatcgtt ccgcgaatac tcgccagggc cgacgccagg ctgtttcgtt
     3181 cgtcgatcca gacgaggacc cgcgagcgaa cttcgctctt ggcgccgatc ttcgtcggct
     3241 cagcagccgc agccgccaac gcgtcgatct gctgcgccag cagttgccgc tgcgggtcct
     3301 ccgaccccag ccgggccagc gactggcgga gcgcggcaat cgccggctga gcttcctgcc
     3361 agcgctgccg gttcttttcg atccgctggt cgaggtcgtg ttcgacggcc agtagccgct
     3421 cgcggcgttt gggcagttcg gccaggtcgc gacgcagccg caactcgtcg gccagcaccc
     3481 acacgcgatc cacccttgcc aggccgtgct gcgccaggac ggcggcggga tcgagttcgg
     3541 caacgtccgc cgcggcggcg ggccaggcag ccgtacacgc cagcaaggcg gcaacgcgcg
     3601 gcagactcgc gagaggaggc atccctggac cccaacctgg gacgagccgg gctagtttga
     3661 agttccgacg gttgatctta gccgctgagc cgccagactg ccagcgctgc tatgacgggt
     3721 gcggcggtga ccgtctgctc tg
//
LOCUS       DASRJB010000691         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1890256, whole genome shotgun sequence.
ACCESSION   DASRJB010000691 DASRJB010000000
VERSION     DASRJB010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890256"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2352
                     /locus_tag="VFB80_18370"
     CDS             <1..2352
                     /locus_tag="VFB80_18370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN35804.1"
                     /translation="TVTGNIRRPGEKTSVHSEGQLVWRYHPELKKYEIFAEGGGNAFG
                     LEIDSKGRIYSGHNGGNTRGFHYVQGGYFQKSFGKHGELSNPFTFGYFEAMAHHNVPR
                     FTHTFVIYEAEALPQPYRGRLFGVAPLLSHVVMSDVLPDRSSFKTKDVGYALESSDTW
                     FRPVDIQVGPDGCIYVADFYEQRIDHSSHYQGRVHKESGRIYRLSAKGSATVGPKFDL
                     TKESGPELVRHLADNNEWFRQAARQELMRRGDRTIGFAAATRLKSAPGNHPVELLWVL
                     ATTRGLTDASAVDLLQHADPYVRLWSVRLACDDGQVSDELAKALAELAYREPHVEVRS
                     QLAASARRLPAAQALAIVKNLASRSEDAGDIHIPLLLWWAIEAKADKDRETVLALFAD
                     KSFWDQPIVGKHLSERLMRRYAATGLRKDLLTCAKLLELAPSKEHAGRLMAGFEAAFE
                     GRTIANLPVELAAAMTKAGAASPTLRLRQGDQAAVAESLALIADEKADAAKRQQLVAV
                     FGTIQQPSCVSVLLKVATESRNDQLRSAALGSLISYADASIAPAVVKLYRDLPDAVRD
                     VAQSLLASRKESAMLLLTAVDSGQIDPKTIAEPTVRKLLLHDAPQIAQLCKKHFGELS
                     GPSPEELRAAIEKLAGVIAAGSGNPYQGKQLFGQSCGKCHVLFAQGGKIGPDLTSYKR
                     DDLRGVLLNVVNPSAEIREGFENYVARTADGRTLTGFIADQDPSIVVLRGADGQNITL
                     PRADIEDLRASKTSLMPENQLKELTDQQIRDLFAYLRATQPLP"
     gene            complement(2436..3047)
                     /gene="tmk"
                     /locus_tag="VFB80_18375"
     CDS             complement(2436..3047)
                     /gene="tmk"
                     /locus_tag="VFB80_18375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZN35805.1"
                     /translation="MFFSFDGVDGVGKSTQIRLLAEALRQQGREVVTCRDPGSTALGE
                     RLRDILLNHHGTPIHRRSEMLLYMAARAQLVEEVIRPELAAGKTVISDRYLLANVVYQ
                     AHGGGLSADDVWEVGRVTVAGVMPRLVFLLDMPAERAAQRINRQPDRMEAQGLAYLER
                     VRQGFLAEAALFPDQVAVINADQPIEAIHAEVLAAAAQVERRV"
     gene            complement(3149..3568)
                     /locus_tag="VFB80_18380"
     CDS             complement(3149..3568)
                     /locus_tag="VFB80_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35806.1"
                     /translation="MKILCLPLLAAAGALLLASCSNTPSNMCAVRGTLTYKGQPMPQV
                     FLKFEPDDLTKKSTSMAVTDASGKFEMAIGSTPGVYKGKVKVTCDDPLAAMGTKTPVP
                     KEVETAYRELCAKYGTGKSTYEMTIDKPLTDLKLALD"
     gene            complement(3611..>3836)
                     /locus_tag="VFB80_18385"
     CDS             complement(3611..>3836)
                     /locus_tag="VFB80_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HZN35807.1"
                     /translation="IASVNGTQRPMPPNPTTALMASWGQNARTVWSYHPNGVMASLCD
                     GSVQFVPNTIPQATWFAMFTRNGGEAIQLQ"
BASE COUNT          666 a         1268 c         1263 g          639 t
ORIGIN      
        1 accgttaccg gcaacatccg ccgccccggc gaaaaaacgt ccgtccattc cgaaggccag
       61 ctcgtctggc gctaccaccc cgagctgaag aagtacgaaa tcttcgccga aggcggcggc
      121 aacgcgttcg gcctggagat cgacagcaag ggccgcatct acagcggcca caacggcggc
      181 aacacccgcg gcttccacta tgtgcagggc ggctattttc aaaagagctt cggcaagcac
      241 ggcgagctgt cgaacccgtt cacgttcggc tatttcgagg cgatggccca ccacaacgtg
      301 ccgcggttca cgcacacgtt cgtgatctac gaggccgagg ccctgccgca gccctaccgc
      361 ggccgcctgt tcggcgtcgc cccgctgctg agccacgtcg tgatgagcga cgtgttgccc
      421 gaccgctcgt cgttcaagac caaggacgtc ggctacgccc tggaaagcag cgacacctgg
      481 ttccgcccgg tcgatatcca ggtcggcccc gacggctgca tttacgtcgc cgatttctac
      541 gagcagcgga ttgaccactc cagccactac cagggccgcg tgcataagga aagcgggcgg
      601 atttaccgcc ttagcgcgaa ggggtctgcg accgtcgggc caaagtttga tctgacgaag
      661 gagagcggtc cggagttggt ccggcatttg gcggacaaca acgagtggtt ccgccaggcg
      721 gcgcggcagg agctcatgcg ccggggcgat cgaaccatag gctttgccgc cgcgacgcga
      781 ttgaagtcgg ctccgggcaa ccatccggtc gagctgctgt gggtgctcgc cacgacgcgc
      841 ggcttgaccg acgcgtcggc cgtcgacttg ttgcagcatg ctgatcccta cgtccgcctc
      901 tggtccgtgc ggctcgcttg cgacgacggg caagtgagtg acgaactagc caaggccctg
      961 gccgagctgg cctaccgcga gccgcatgtc gaggtccgca gccagctcgc ggcgtcggcc
     1021 cgccggctgc cggcggcgca ggcgctcgcc atcgtgaaga acctcgcgag ccgcagcgag
     1081 gacgccggcg acattcatat tccgctgttg ctctggtggg cgatcgaagc caaggccgac
     1141 aaggaccgcg agacggtgct cgcgctcttc gccgacaaga gcttttggga tcagccgatc
     1201 gtcggcaagc acttgagcga gcggctgatg cggcgctatg cggcgacggg cttgcgcaag
     1261 gatttgctga cctgcgcgaa gctgctggag ctcgcgccca gcaaggagca cgccggccgg
     1321 ttgatggccg gctttgaggc ggcgttcgag ggccgcacga tcgccaatct gccggtcgag
     1381 ctggccgctg cgatgaccaa ggccggcgcg gcctcgccta ctttgcggct gcggcagggc
     1441 gaccaggcag ccgtggccga gtcgctggcg ctgattgccg acgaaaaggc cgacgcggcc
     1501 aaacggcagc agctcgtggc cgtctttggc acgatccagc agccctcctg cgtgagcgtg
     1561 ttgctgaagg tcgcgaccga atcgcggaac gaccagctcc gcagcgccgc cttggggtcg
     1621 ctcatctcgt atgccgatgc atcgattgcc cccgcggtgg tgaagctcta tcgcgattta
     1681 cccgacgcgg tgcgcgacgt ggcccagtcg cttttggcca gccgcaagga atcggcgatg
     1741 ctgctcttaa cggcggtcga ttccggccag atcgatccca agaccatcgc cgagccgacg
     1801 gtccgcaagc tgctgctgca cgacgccccg cagatcgcgc agctctgcaa aaagcacttt
     1861 ggcgagctct ccggcccgtc gcccgaggag ctgcgggccg cgatcgaaaa gctggcgggc
     1921 gtgatcgccg ccggctcggg caatccgtac cagggcaagc agctctttgg ccagagctgc
     1981 ggcaagtgcc acgtgctatt cgcccagggc ggcaagattg gccccgacct gacgagctac
     2041 aagcgggacg acctgcgcgg ggtgctgctg aacgtggtca accccagcgc cgagattcgc
     2101 gagggctttg agaactacgt cgcccgcacc gccgacggcc gcacgctcac cggcttcatc
     2161 gccgaccagg atcccagcat cgtcgtcctc cgcggggccg atggccagaa catcacgctc
     2221 ccccgcgccg acatcgaaga cctccgcgcc agcaagacca gcctcatgcc cgagaaccag
     2281 ctcaaggaac tcaccgacca gcaaatccgc gacctcttcg cctacctacg agccacgcag
     2341 ccgctgccgt agggtgggac cagcgagcgg agcgagcgcc ggcccaccgc tcgagcaatc
     2401 gcgttggtgt gccggcggcc ggaggaccgg cctgatcaca ccctacgctc cacctgagca
     2461 gccgcggcga ggacttcagc atgaatcgcc tcgatcggct gatccgcgtt gatgacggcg
     2521 acctggtcgg gaaagagggc ggcttcggcg aggaagccct ggcggacgcg ctccaggtag
     2581 gccaggccct gggcttccat ccggtcgggc tgccggttga tccgctgggc ggcccgctcg
     2641 gcgggcatgt cgagcaggaa gaccagccgc ggcatgacgc cggcgacggt gacgcggccg
     2701 acctcccaca catcgtccgc ggacagaccg ccgccgtggg cctgatagac gacgttggcc
     2761 agcagatagc ggtcgctgat gacggtcttg ccggctgcca gctccgggcg aatgacttct
     2821 tcgacgagct gggcccgcgc ggccatgtag agcagcattt cgctgcggcg gtggatcggc
     2881 gtgccgtggt ggttcagcag gatgtcgcgc agccgctcgc ccagcgccgt gctgcccgga
     2941 tcgcggcagg tgaccacctc gcggccttgc tgccgcaggg cctcggccaa aagccggatt
     3001 tgcgtcgatt tgccgacgcc atccacgccg tcgaagctga agaacatgcg attggagatt
     3061 gtcagttgtc cgtagtcagt tgtcggtgat gggacgccat ctttcaactg accactgacc
     3121 actgacaacc ggccactggc atctgtggtc aatccagcgc cagcttcagg tccgtcagcg
     3181 gcttgtcgat cgtcatttcg taggtggact tgccggtgcc gtacttcgcg cacaactcgc
     3241 gataagcggt ttcgacctcc ttggggacgg gcgttttggt gcccatcgcc gccagcggat
     3301 cgtcgcaagt gactttcacc ttgcccttat acacgccggg cgtcgagccg atcgccatct
     3361 cgaatttgcc gctagcgtca gtgaccgcca tgctcgtgga ctttttcgtc agatcgtccg
     3421 gctcaaactt gaggaagacc tgcggcatcg gctgaccctt gtaggtcagc gtcccgcgga
     3481 ccgcgcacat gttggaaggg gtgttgctgc agctggccag cagcagcgcg ccggctgccg
     3541 ccaggagggg caggcagaga atcttcatgg cagcctcgga gaaacgaagt ccggccagtg
     3601 cggcggggag ttattgcagt tggatcgcct cgccgccgtt gcgagtaaac atggcgaacc
     3661 aggtcgcctg gggaatcgtg ttgggaacga attggacgct cccgtcgcag agcgacgcca
     3721 tcacgccgtt gggatggtag ctccacaccg tgcgcgcatt ctgcccccag gaggccatca
     3781 gcgcggtggt tggattcggc ggcatcggcc gctgggtgcc attgacggac gcaatc
//
LOCUS       DASRJB010000692         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_202383, whole genome shotgun sequence.
ACCESSION   DASRJB010000692 DASRJB010000000
VERSION     DASRJB010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202383"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1840
                     /locus_tag="VFB80_18390"
     CDS             386..1840
                     /locus_tag="VFB80_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35808.1"
                     /translation="MSRVAMLSFSLSLLLSFSSTADAQTTKKTFRAGAYAIDISPLKL
                     PVIINGGMTERTADKITDRLHARCLVLDDGTNQVALVVVDSCMVPRDLLDEAKEAARK
                     ATGIPTSHMLISATHTHSAPSVHGCLGSDKDEEYARFLPPQIAKGIIEAHKRLQPARI
                     GWAFGKDEKNVACRHWVMKPGTAQTNPFGGTKDDLVQMNPNVNNPNAIRPTGTPDPAV
                     PVISIQTPDGQPLALYSAYSVHYVGKTTPVSADYFPLYCDNMARHLKADRNPAFMAAL
                     ANATSGDTWLMDYSQPVRREYTLESVAADVAAAALEACGRIQYFDWVPLVMQESLLEC
                     NVRMPSAEEVAAAKEFMKSFEGKKPKNVPEVYARETVLLSQMPSTRELKIQALRIGSL
                     GIGTIPNETYASTGLFIKEQSPLKETFVVELANGCEGYIPPPELHPLGGYTCWRARTA
                     CLAPESERQIRSRVLELLGAVAKERAGEQPVASK"
     gene            complement(1962..2264)
                     /locus_tag="VFB80_18395"
     CDS             complement(1962..2264)
                     /locus_tag="VFB80_18395"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZN35809.1"
                     /translation="MSVLEDSTDTSLIVRVQARDSAAWQTLVDLYAPWIYGWTRRYGL
                     TPEDGADVTQDTLLAAHRSIEQFVATRRTIPFAAGYGRSSVTRFATCSDASGGRQA"
     gene            complement(2372..3619)
                     /locus_tag="VFB80_18400"
     CDS             complement(2372..3619)
                     /locus_tag="VFB80_18400"
                     /inference="COORDINATES: protein motif:HMM:NF018936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 family protein"
                     /protein_id="HZN35810.1"
                     /translation="MGWLDFLFGPPSKDKFARLVMAELRKLNQASELKYNSEQFLIER
                     GSEGFINLANLYHEYCQAPRRERRMVLDRFIRGCIGTTGFELPQDFDDVHPDLLPVVR
                     SRFYLESVALQSRARGGEGFHVPPQQSIGDHLALSLVYDLPQAMRSIIQEDLDQWDVT
                     FYEALEAARRNLEQMGNVAFASLQGKEGDGVYISATGDNYDASRLVMLDLVRKMPVRG
                     DYIAMVPNRDTLVITGSDDSAGLEVMCKLAEESFEKPRPISTIALRLEGDSWESWLPP
                     RDSTAYAKFHELRLRTIGMEYNDQKELLDQIHEKTGEDVFVASFSALQNKQTGRISSY
                     SVWSQGIASLLPETDDVVFLRGASDGNEDVQLAAAGSWQRVRDVAGDLMQPQGTYPER
                     YRVLEFPSAQQLAAIGKGDWPEL"
     gene            complement(3710..4390)
                     /locus_tag="VFB80_18405"
     CDS             complement(3710..4390)
                     /locus_tag="VFB80_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35811.1"
                     /translation="MGNTSSDTRTVIVIVAVVGLLSCGCMCMPLGLLGLGVGLPAAIQ
                     VQKAAQAVAENQPPPSEFAAFPPEFKPVIEPPPVISPPNLTPPSFTPTPVPNIPPLDA
                     PLQFTPPAITPPAITPPPPTPSPRPPRGGAGPGGLVALSEQQRRGIYFSATIHRRMLE
                     SIEEQRVKTRERGGNADFLEQMVQSTRERMNEQLDRLCERNKLSRAELDQIIAEGDQQ
                     GWGGPRRK"
     gene            complement(4491..5393)
                     /locus_tag="VFB80_18410"
     CDS             complement(4491..5393)
                     /locus_tag="VFB80_18410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZN35812.1"
                     /translation="MQAPSASPLPSTFGLTEERRVALERLFGRIGDVTTLPAAAQRVL
                     QLTEDENSDPDQLREAIQSDPVLVARILRRLNSSYYALSHRVSDLKAAISLLGLREIR
                     NLALTVFVSRLFEGGAAHGTYRREQLWSHSVAVAVASRLVARVCGRGAGEEAYIGGLL
                     HDIGLILLDQTLRRHFLKVIDALDAETPTHIVENRLLTFDHAILGGFVARKWNFPEPV
                     ADAITFHHQPWCYNGTHKDLVHVVAVANYFCSRAGVTSLGLHNVSPPPDEVYAGLGLD
                     QISLAIIWDELDTALDKATALAAS"
     gene            5656..>6789
                     /locus_tag="VFB80_18415"
     CDS             5656..>6789
                     /locus_tag="VFB80_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN35813.1"
                     /translation="MSKGKDLGILERLKGGEVAGLTARAPTHEPEAAEGDPDAKLNEA
                     LSRIQQLAGNEAKKPEPAPLKPAAHAATPAPLPPPAEHQATAPDKFVPMEPASIRAAG
                     LTDSEVEALILKYLLSRGDCTGRDAADQIRLPFILLDPLLRQLKFDQLLVYRGSAPMN
                     DYVYQLTDLGRERARRFMDHCTYYGAAPVSLTDYIEGVKAQSLENQHPTADDLKRAFW
                     DILVSERMLRRLGPAINSGRGLFLYGSAGNGKTTIAERLTRSFGQFIWMPRAIGVDGE
                     IIRLFDPVNHEECPLPPGTGLWDQSKIDRRWIRIRRPTIVVGGELTMDSLEVTLNRST
                     GISEAPLQLKSNCGTLVIDDFGRQKMSTDTLLNRWIVPLEKRFD"
BASE COUNT         1200 a         2311 c         2151 g         1127 t
ORIGIN      
        1 tgttgccgct gggcgcagtc aaccccacgc cggtgatgac gatccgctgc gaggcgggca
       61 acttatcggg agcatgcacg gacatgacgc ttcctgggcg agcgcggcaa ctgaccgcta
      121 ccaggacgta ccgaacgcaa accaccgaag ttaacggtcc actgcacgac tggcaagcgg
      181 cgggatcgcg gcgaaagcgc cgccgagtgt ggcgtcataa ctgccgtccc aaccagcact
      241 taccgcggca gccgccgtga cccgttgcag cggtgcggcc ggtttgttca tctgcgattg
      301 acgcggagtt tacacgccgc tgaccctttc cccgcctatc tcgatccgct agaaagcgct
      361 gcatccccgc cacgaggagc ccgcaatgtc ccgcgtcgcc atgctctcct tctctctttc
      421 tctccttctc tccttcagct ccacggccga cgcccagacc accaagaaga ccttccgcgc
      481 cggcgcctac gccatcgaca tcagccccct caagctgccc gtgatcatca acggcggcat
      541 gaccgagcgg accgccgaca agataaccga tcggctgcac gcccgctgcc tcgtgctcga
      601 cgacggcacg aatcaggtgg ccttggttgt cgtcgatagc tgcatggtgc cgcgcgacct
      661 gctcgacgaa gccaaggagg ccgcccgcaa ggccactggc atccccacta gccacatgct
      721 gatctcggcc acgcacacgc actcggcccc cagcgttcat ggctgcctgg gctccgacaa
      781 agacgaagag tacgcccgct tcctcccgcc gcagatcgcc aaggggatca tcgaggctca
      841 caagcggctg cagccggccc gcatcggctg ggcgttcggc aaggacgaga agaacgtggc
      901 ctgccggcac tgggtgatga agcccggcac ggcccagacc aaccccttcg gcggcacgaa
      961 ggacgacctc gtgcagatga acccgaacgt caacaatccc aacgccatcc gcccgacggg
     1021 cacgcccgac ccggcggtgc ccgtgatctc gatccagacg ccggacgggc agccgctggc
     1081 gctgtattcg gcctattcgg tgcactacgt cggcaagacg acgcccgtca gcgccgatta
     1141 ctttccgctg tactgcgaca acatggccag gcacctcaag gcggatcgca atccagcgtt
     1201 catggcggcg ctggccaatg cgactagcgg cgacacctgg ctgatggatt actcccagcc
     1261 ggtgcgccgc gagtacacgc tggaaagcgt cgccgccgac gtagcggccg cggcgcttga
     1321 ggcctgcggg cggatccagt acttcgattg ggttccgctg gtgatgcaag agtcgctgct
     1381 ggagtgcaac gtgcggatgc cttcggccga ggaagttgcg gcggcgaagg agttcatgaa
     1441 gtcgttcgaa ggcaagaagc ccaagaacgt gccggaggtc tatgcccgcg agacggtgct
     1501 gctctcgcaa atgccgtcga cgcgcgagct gaagattcag gccctgcgga ttggcagcct
     1561 cggcatcggc acgatcccca acgagaccta cgccagcacg ggcctcttca tcaaggagca
     1621 gagcccgctc aaggagacgt tcgtcgtcga actggccaac ggctgcgaag gctacattcc
     1681 tccgcccgag ttgcacccgc tcggtggcta cacctgctgg cgcgcccgca ccgcctgcct
     1741 tgctcctgag tcggagcggc agatccgctc ccgcgtgctg gagttgctgg gcgcggtcgc
     1801 caaagagcgc gcgggcgagc agccggtcgc cagcaaatag cggtcaattg gatttggtcc
     1861 aggcgtccca ggttttccgc cagtcccagg ctttcgcagc gggcggggcg agggcgccta
     1921 cgtgctgcgg cagcgactcg gcggtgccgg cgccgctgtg cctacgcttg cctcccgccg
     1981 ctggcgtcgc tgcacgtcgc gaatcttgtt acggacgatc gtccataacc agccgcgaaa
     2041 ggaatcgtcc ggcgcgtggc gacgaactgc tcaattgagc gatgcgccgc cagcagcgta
     2101 tcctgcgtga cgtccgcccc gtcctccgga gtcagcccgt accgccgcgt ccagccgtaa
     2161 atccacggcg cgtacaaatc gaccagcgtt tgccaggcag ccgagtctcg cgcctgaacg
     2221 cgcacgatca ggctggtatc ggtcgagtcc tcaaggacgg acatgcgcgc cagacccaag
     2281 ggccgaatcg gccaggcgaa gaactgcccg ccagcctatt caaccgctcg cccgtgagca
     2341 acttgaagtg atcgtagccg ttggcaggcc gttacaactc cggccaatcc cctttgccga
     2401 tcgccgccag ctgctgggcc gaggggaact ccaggacgcg gtaccgctcg ggataggtcc
     2461 cctgcggctg catcaggtcg ccggccacgt cgcggacccg ctgccagctg ccggcggcgg
     2521 cgagctgcac gtcttcattg ccgtcggacg cgccgcggag gaagacgacg tcgtccgtct
     2581 ccggcaacag cgacgcgatt ccctgcgacc agacgctgta actgctgatc cggccggtct
     2641 gcttgttttg cagggcgctg aagctggcca cgaacacgtc ctcgcccgtc ttctcgtgga
     2701 tctggtcgag cagctccttc tggtcgttgt attccatgcc gatcgtccgc agccgcagct
     2761 cgtggaactt cgcgtaggcg gtcgagtcgc gcggcggcag ccacgattcc cacgagtccc
     2821 cctccagccg cagggcgatc gtcgaaatcg gccgcggctt ctcgaacgac tcctcggcca
     2881 gcttgcacat cacctccagg cccgcggagt catccgagcc cgtgatcacc agcgtgtcgc
     2941 ggttggggac catcgcgatg taatcgccgc ggacgggcat cttccgcacc aggtcgagca
     3001 tcaccagccg cgaggcgtcg tagttgtcgc cggtggccga gatgtaaacg ccgtccccct
     3061 ctttgccctg caggctggcg aaggcgacgt tgcccatctg ctccaggttg cggcgggccg
     3121 cttccagcgc ctcgtaaaac gtcacgtccc actggtccag gtcttcctgg atgatgctcc
     3181 gcatcgcttg cggcaggtcg tagaccaacg acagcgccag gtggtcaccg atcgactgct
     3241 gcggcgggac gtgaaagcct tcgccgccgc gggcccgcga ctggagtgcg accgactcca
     3301 ggtagaaccg cgaccgcacg accggcagca ggtccgggtg cacgtcgtcg aagtcctgcg
     3361 gcagctcgaa gcccgtcgtg ccgatgcagc cgcggatgaa ccggtcgagg accatccgtc
     3421 gctccctgcg cggggcctgg cagtattcgt gatagaggtt ggcgaggttg atgaagcctt
     3481 cgctgccacg ttcgatgagg aactgctcgg agttgtattt cagctcgctc gcctggttga
     3541 gcttgcgcag ctcggccatc accaatctgg cgaacttgtc cttgctcggc gggccgaaga
     3601 ggaaatcgag ccaacccacg gcgaaaactc ctgaccgatc actgtccctt gcgactctac
     3661 cgcattgcga tcgggcggtc gagtcgcaat cgtacctgca tggagtggct cacttcctgc
     3721 gcggtccgcc ccagccctgc tgatccccct cggcgataat ctggtccagt tcggcccgcg
     3781 agagcttgtt ccgctcgcag agccgatcga gctgctcgtt cattcgctcc ctggtgcttt
     3841 gcaccatttg ctcgaggaag tcagcgtttc cgccgcgctc gcgagtcttg acccgctgtt
     3901 cttcgatcga ctccagcatc cggcggtgga tggtggccga aaaataaatg ccccgccgct
     3961 gttgctcgga gagcgccacc aggccgcccg ggccggcgcc tccacgcggc ggtcttgggg
     4021 atggcgtcgg cggaggcggt gtgatcgcgg gcggcgtgat cgcgggcggc gtgaattgca
     4081 gcggtgcatc cagcggcgga atgttgggca ccggcgtcgg cgtaaaggac ggtggcgtca
     4141 ggttcggcgg cgatatgacg ggcggcggct cgatgaccgg cttgaactct ggtgggaacg
     4201 cggcaaactc gctcggcggc ggctgattct cggcgactgc ctgggccgcc ttctgcacct
     4261 gaatcgccgc gggcaggccg acgccgagcc ccagcagccc cagcggcatg cacatgcagc
     4321 cgcaggacag caggcccacc accgcgacga tgacaatcac cgtccgcgta tccgacgatg
     4381 tgttccccat gcaattgtcg taaccgcctg cggggcggcg agcaagatca gtgccattcc
     4441 gggtaatccg ctcgctccgc gagcggcctc acgcggagcg tgaggactat ttacgacgcc
     4501 gccagggcgg tcgccttgtc gagcgcggta tcgagctcgt cccagatgat cgccagcgag
     4561 atctggtcca ggcccagccc ggcatagacc tcgtccggcg gcggcgacac gttgtgcagg
     4621 cccagcgaag tcacgccggc ccggctgcaa aaatagttcg ccaccgccac gacgtgcacc
     4681 aggtccttgt gcgtgccgtt gtaacaccag ggctgatggt gaaacgtaat cgcgtcggcc
     4741 accggctcag gaaaattcca cttgcgggcc acgaacccgc ccaggatcgc gtggtcgaac
     4801 gtgagcaggc ggttctcgac gatgtgcgtc ggtgtttccg cgtcaagggc gtcgatcacc
     4861 ttcaggaagt gccgccgcag cgtctggtcc agcagaatca gcccgatatc gtgcaacaag
     4921 ccgccgatat acgcttcttc gccagcaccg cgcccgcaga cccgcgccac caagcgcgac
     4981 gccaccgcga cagcgaccga gtgactccac aattgttcgc ggcgatacgt tccgtgggcc
     5041 gccccgcctt cgaacagtct cgagacgaac accgtcaggg ccaggttgcg aatttcgcgc
     5101 agccccagca ggctaatcgc ggccttcaga tccgaaacgc gatggctgag cgcgtagtac
     5161 gacgaattca gtcgccgcag gatccgggcc accagcaccg ggtccgactg aattgcctcc
     5221 ctcaactgat cggggtcgga gttctcatct tccgtcagtt gcaggaccct ttgggccgca
     5281 gccggcagcg tcgtcacgtc gccgatccgc ccgaacagcc gttccagggc cacgcggcgc
     5341 tcttccgtca ggccaaacgt ggagggcaga ggactggcgg agggagcttg cacaaactca
     5401 atcccaacga gtgcagaaca atcgccatcc aaaacctagt ccaccagcag actgggtagg
     5461 ccccagcaag ctggttgctt atcgcgggtg ccgccgcgcg ggcggcacga tcgtcaagtt
     5521 cgttccaatc gacccagcat cgggcggctg tgcgggcagg ccgccgggcg gagtttgctc
     5581 ctgttctgcc gtcgacctag ggtttcgctg gaccggttag tagagctgca ttccccatcg
     5641 gcaaggcgtt gcaatatgag caagggtaaa gacttaggca ttttggaacg gctgaagggc
     5701 ggcgaggtgg ctgggctgac ggccagggcg cccacgcatg agccggaagc ggcggagggc
     5761 gatcccgacg ccaagctcaa cgaggcgctc agccgcattc agcagttggc cggcaacgaa
     5821 gcgaagaagc cggagccggc acccctgaag ccggccgctc acgctgcgac gcccgcgccg
     5881 cttcccccgc ctgccgagca ccaggcaacc gcgcccgaca agttcgtgcc gatggagccg
     5941 gcgtcgattc gggcggcggg actgaccgac agtgaagtcg aggcgctcat tctgaagtac
     6001 ttgctgtcgc ggggcgactg cacgggccgc gatgccgccg accagattcg cctgccgttc
     6061 atcctgctgg acccgctctt gcggcagctg aaattcgatc agctgctggt ctatcgcggc
     6121 tcggcgccga tgaacgacta cgtctatcag ctcaccgacc tgggccgcga acgggcccgc
     6181 cggttcatgg accactgcac gtactacggc gctgcaccgg tatcgctgac ggactacatc
     6241 gagggcgtga aggcccagtc gctcgagaac cagcacccca cggccgacga cctgaagcgg
     6301 gccttctggg acattctcgt cagcgagcgg atgctccgcc gcctcgggcc ggcgatcaac
     6361 tcgggccgcg gcttgttcct ctatggctcg gcgggcaacg gcaagacgac catcgccgag
     6421 cggctgacgc ggtcgttcgg gcaattcatc tggatgccgc gggcgatcgg tgtggacggc
     6481 gagatcatcc gcctgttcga cccggtcaac cacgaggaat gcccgctgcc gcccggcacc
     6541 ggcctgtggg accagtcgaa aatcgaccgc cgctggatcc gcatccgccg gccgacgatc
     6601 gtggtcggcg gcgaattgac gatggacagc ctggaagtca cgctcaaccg ctcgaccggc
     6661 atcagcgaag ccccgctgca gctcaagagc aactgcggca cgctggtcat cgacgacttc
     6721 ggccgccaga agatgagcac cgacaccttg ctcaaccgct ggatcgtgcc gctggaaaag
     6781 cggttcgac
//
LOCUS       DASRJB010000693         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3026020, whole genome shotgun sequence.
ACCESSION   DASRJB010000693 DASRJB010000000
VERSION     DASRJB010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026020"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..680)
                     /locus_tag="VFB80_18420"
     CDS             complement(84..680)
                     /locus_tag="VFB80_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35814.1"
                     /translation="MHRSWPVVALAVVVASFSGGPVLAEPPAADAPQSAERQFVTVEL
                     SVVDVSLTKLRALGFDWEAVSKTLQPGFPDPKQLKFGAGKQLEGFLQLLKQYNIGEVV
                     SNPKLMTLNGRPASVDISPHLKLDVVPIVLGSGRIRVEHRLELPQRKLKTDSAVEVDP
                     GQAVVAGQVRSEKKDAAGKLHETATLIIVRADTSTKLK"
     gene            complement(776..2233)
                     /locus_tag="VFB80_18425"
     CDS             complement(776..2233)
                     /locus_tag="VFB80_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35815.1"
                     /translation="MLAIISDLHINDGTTGQLLPAAAIDLVCERLCDLAWRASWRSDG
                     CYRPIDRIDLVLLGDTLDLMGSRRWLAAPCRPWDDHQSPAVIDAVTGIVEETLRRNVD
                     CIRTLRALATESTVSLPPATAAGQPVLEAEELPVAICTHYMVGDRDWPLHLKGTQYDL
                     IRHKVTHHLGLVTPYNKPFPHEPAECEQLQEALRQHRVLARHGDIFDPLSFADDRDCA
                     SLDGVLAIELIARFQQHIESELAEQMPAATWSAICEIDQIRPMLLVPAYLDSVLERTA
                     TPAIKGRIKRIWDYMVEQVLHLEIVRRLASASPIDLIDGLSSALKFASRDSQSWIDRT
                     LGFTNALRGAGNGSYAAQAMAEADFRNRRARHIVYGHTHQHEIVPLDASHADGYVLTQ
                     TYFNAGTFRRSYLPTQMLAGHHELSACDSFSLLAFYQGDERSGRAYEAWTGMLAPQAI
                     DAAAAMPAPPITTAAPIRAPQFAGSSRAASLVRGY"
     gene            2796..3389
                     /locus_tag="VFB80_18430"
     CDS             2796..3389
                     /locus_tag="VFB80_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN35816.1"
                     /translation="MSANTKTVFTTGEAAKICKVSQQTIIRCFDNGSLKGFRVPGSRF
                     RRIPRNELYSFMRDNGIPTDALESGKRKVLIVDDDVELVELMSDAFARDGRFEIKTAN
                     NGFDAGMLVKEFRPDLVVLDIMLPDINGKEVCQRVRADSTLEAVKVICISGMVEADKV
                     ADLRAAGADDFLQKPFTVDKLLDRSCDLLELEKPVLA"
     gene            3477..4193
                     /locus_tag="VFB80_18435"
     CDS             3477..4193
                     /locus_tag="VFB80_18435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN35817.1"
                     /translation="MQAAVESPTHADFLEAVERAKLQALYQFAYGLSHEINNPLANIA
                     TRAQTLLVDESDPERRRKLATIVQQAFRAHEMIADLMLFARPPALRLEAVDLVQLADC
                     VVGELQEQAREQGTRLTRTGESGPLLATADPTQLGVALKALVQNALEAVRVGGAVEVG
                     VASGAELSVSDTGPGIPAEIRPHIFDPFFCGREAGRGLGLGLSKAWRIAELHGGTIEV
                     ESEPGQGARLAILLPAPDRG"
     gene            4196..4636
                     /locus_tag="VFB80_18440"
     CDS             4196..4636
                     /locus_tag="VFB80_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35818.1"
                     /translation="MALTGSRFGQLAAILPAAAVSLLYLLLLGHRHDYLGHYAAGYGA
                     SFAALAVWLRSLSRAKFESLAPRSILPGCVLCILAGAITEATIFRLARFDEIDFCNQS
                     LGAVMAGIAALTLVREPRPADREFDIATITGIAYLGIGGWFAFH"
     gene            4623..5030
                     /locus_tag="VFB80_18445"
     CDS             4623..5030
                     /locus_tag="VFB80_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35819.1"
                     /translation="MRSTSLFTAALLLLVAAAFCAGNLWFAAVRSTIPLELDGVVRSK
                     ELRREKHPGVDDVYLLELDRGWIVVDRSVFDSVQPGDLLRKPRWSRQLQVGSRTVRLI
                     WSADMRGMLVAMPCVFVVVAATIAAVTRQARAS"
BASE COUNT          976 a         1706 c         1848 g          994 t
ORIGIN      
        1 gacttcacat taggcgcaag gtattgatcg acaaagagtt agctccgtga tcgcggcaag
       61 aagcgctcgc tcgcgagtcc ttgctatttg agcttcgtgg aggtgtcggc tcgcacgatg
      121 atgagcgtgg ccgtctcgtg aagcttgccc gcggcgtcct tcttctcgct gcggacctgg
      181 ccggcgacaa cggcttggcc gggatctact tcgacggccg aatcggtttt aagtttccgc
      241 tgaggcagtt ccaggcggtg ctcgacgcga atgcggcccg agccgagcac gatcggcacg
      301 acgtcgagct tcaagtgagg actgatgtcc acggaggccg ggcgcccgtt cagcgtcatg
      361 agcttgggat tggagacgac ttcgccgatg ttgtattgct tcaagagctg caaaaagcct
      421 tcgagctgct tgccggcgcc gaacttgagc tgcttcggat ccggaaagcc cggctggagc
      481 gtcttcgaga ccgcttccca gtcaaaacca agggcccgca acttcgtcag cgaaacgtcc
      541 accacggaca actcgactgt gacaaactgc ctctcagcag attgaggcgc gtcggcggcc
      601 ggtggttcgg cgaggacggg accgccagag aacgaggcga ctacgacggc cagagccaca
      661 acgggccatg agcgatgcat acggcgcctt aggagtgcga aacactaaac acaaacacca
      721 aacactttgg ccgggtgttt ggtgttcgtg tttggtgatc agacgcgttt tgaaatcaat
      781 acccgcggac gagagacgcg gcgcggctgc tgccggcgaa ctgcggggcg cggatcgggg
      841 cggccgtggt gataggcggc gcgggcatcg cagccgcggc gtcgatcgcc tgcggggcga
      901 gcatgccggt ccacgcttcg taggcccggc cgctgcgctc gtcgccttga tagaaggcca
      961 ggagcgaaaa gctgtcgcag gcggaaagct cgtggtggcc ggccagcatc tgcgtcggca
     1021 ggtagctgcg gcggaacgtg ccggcgttga aataggtttg cgtcaggacg tagccgtcgg
     1081 cgtggctggc gtcgagcggg acgatttcat gctggtgcgt gtggccatag acgatgtgcc
     1141 gggcgcggcg attgcggaaa tcggcctcgg ccatcgcctg cgcggcatac gaaccattgc
     1201 ccgcgccccg cagcgcgttt gtgaagccca gcgtgcggtc gatccagctc tgcgagtcgc
     1261 ggctggcgaa cttgagcgcg gacgacaggc cgtcgatcag gtcgatcggg ctggcgctcg
     1321 cgaggcgacg gacaatctcc aggtgcagca cttgctcgac catgtagtcc caaatccgct
     1381 tgatgcggcc cttgatcgcg ggcgtcgcgg tgcgctccag gacggagtcc aggtatgccg
     1441 gcaccagcag catcggccgg atctggtcga tttcgcagat cgccgaccag gtcgcagccg
     1501 gcatctgttc ggccagttcg ctttcgatgt gctgctggaa gcgggcgatc agctcgatcg
     1561 ccagcacgcc atcgaggctc gcgcaatcgc ggtcgtcggc gaagctgagc ggatcgaaaa
     1621 tgtcgccgtg gcgggccagc acgcggtgct ggcggagcgc ctcttgcagc tgctcgcact
     1681 cggccggctc gtgcgggaac ggcttgttgt agggcgtgac cagccccagg tgatgcgtca
     1741 ccttgtggcg gatcaggtcg tattgcgtgc ctttgaggtg cagcggccag tcgcggtcgc
     1801 cgaccatgta gtgcgtgcag atggcgaccg gcagctcctc ggcttccaga accggctggc
     1861 ccgcggccgt ggccggcggc aggctgaccg tggattcggt ggccagcgcc cgcagcgtgc
     1921 ggatgcaatc gacgttgcgg cggagcgttt cctcgacgat gcccgtgacc gcgtcgatca
     1981 ccgcaggcga ctggtggtcg tcccacgggc ggcacggcgc ggccagccat cgccgcgagc
     2041 ccatcaggtc gagcgtatcg cccagcagca ccaggtcgat gcgatcgatc ggccggtagc
     2101 agccatccga gcgccacgac gcccgccagg ccagatcgca caggcgttcg caaacgaggt
     2161 cgatcgccgc cgccggcagc agctgaccgg tggtgccgtc gttgatgtgc aggtcgctaa
     2221 tgatcgcgag catgggaacg tccttgttct ggctcaaagc cgcgactcgt gtcgcggtca
     2281 acgtctcatt ggtcacggcg acaggcagac tggtcgcgct agggtgactc gacttttcta
     2341 tcggctgcgg gctacgtggg acgtgaggat tgttcgggca aaagtgtctg cggagtgtgc
     2401 ggcaaatggg ccggaaggcg tgaatttgcg ggatagcgaa aatttgaggc gggcggcatg
     2461 aaatgcggca agtcattgga gggaaagaac ttgcgcccaa catttcgata ttgacgcgcg
     2521 gggggtggcc gatataattc ctcgcggttg gaaagttcgg caagctcggc aaagttgcgg
     2581 aagcgggccg aacgggcggt cccggccagt gtggtgattg tggttcggcg gttaccaagc
     2641 aattgactgc ccggcaaggc aactcggccg gcggtttcat agacggagtt tgggactagc
     2701 aacgacgtgg caatcacgga gggttggagg ccgcagggtc gcgtcctctt taacagctag
     2761 tccaaccatc gaaacggaag ggatggttca cagccatgtc agccaatacc aagacggtgt
     2821 tcacgaccgg cgaagcggcc aagatctgca aagtcagcca gcagaccatt attcgctgct
     2881 tcgacaacgg ttcgctcaag gggttccgcg tgccgggcag ccggttccgc cgcatcccgc
     2941 gaaacgagct gtactcgttc atgcgggaca acggcattcc gaccgacgcg ctggaaagcg
     3001 gcaagcggaa ggtgctgatc gtcgatgacg acgtggagct ggtcgagctg atgagcgacg
     3061 ccttcgcccg cgacggccgc ttcgagatca agacggccaa caacggcttc gacgccggca
     3121 tgctggtcaa ggagttccgg ccggacctgg tcgtgctcga catcatgctg cccgacatca
     3181 acggcaagga agtctgccag cgggtgcggg ccgacagcac gctggaggcc gtgaaagtca
     3241 tctgcatctc cggcatggtc gaggccgaca aggtcgccga cctgcgggca gccggggccg
     3301 acgacttttt gcagaagccg ttcaccgtcg ataagctgct cgaccgctcc tgcgatctcc
     3361 tggagctgga aaaacccgtg ctggcctgag gcccgacgga gtgacggagg gaaggagaga
     3421 cggagcgagt ttgttagtcc ctctgtccct ccttcgctca ttcactccgt catgccatgc
     3481 aggccgcggt cgaatcaccg acacacgccg atttcttaga ggcggtggag cgcgcgaagc
     3541 tccaggctct ctaccagttc gcctacggcc tgagccacga gatcaataac ccgctggcca
     3601 acattgccac gcgggcccag acgctgctgg tggatgagag cgatcccgag cgccgccgca
     3661 agctggcgac catcgtccag caggcgttcc gggcccacga gatgatcgcc gacctgatgc
     3721 tcttcgcccg cccgccggcg ctgcgcctgg aggcggtcga tctggtgcag ctcgccgatt
     3781 gcgtcgtcgg cgaacttcag gagcaggccc gcgagcaagg cacgcgcttg acccgcaccg
     3841 gcgagagcgg cccgctgttg gccaccgccg atccgacgca gctcggcgtg gccctgaagg
     3901 ccctggtgca gaacgcgctg gaggcggtgc gggtgggcgg cgccgtggaa gtcggcgtgg
     3961 cgtcgggcgc ggaattgtcc gtctccgaca ccggccccgg catcccggcc gaaatccgcc
     4021 cgcacatctt cgatccgttc ttctgcggcc gcgaagccgg ccgcggattg ggcctgggcc
     4081 tttccaaggc ctggcggatc gcagaactgc acggcggcac gatcgaggtc gaaagcgagc
     4141 ccggacaagg cgcacggctg gcgatcctgc tgccggcgcc ggaccggggt tagccgtggc
     4201 gctcactggc agccgatttg gccagctcgc ggcgatcctt cccgcggctg ctgtgagcct
     4261 gctgtacttg ctgctccttg gacaccgtca cgattacctg ggccattacg ccgccggcta
     4321 cggcgcgagc ttcgccgcgc tggcggtctg gctgcggtcg ctgtcgcgag cgaaattcga
     4381 atcgcttgcg ccgcgcagca ttctgcccgg ctgcgtgctg tgcatcctcg ccggcgcgat
     4441 caccgaagcc acgattttcc gcctcgcccg cttcgatgag atcgactttt gcaatcaaag
     4501 tctcggcgcg gtaatggccg gcatcgcggc tctgacgctt gttcgcgagc cgcgacctgc
     4561 cgaccgcgaa ttcgacatcg cgacgatcac tgggatcgcc taccttggaa tcggaggctg
     4621 gtttgcgttc cactagcctg ttcacggccg ccttgctgct actcgtcgcc gcggcgttct
     4681 gcgccggcaa cctctggttc gccgctgtcc gctcgacgat tccgctggaa ctggatggcg
     4741 tcgtccgcag caaggagctg cgccgcgaaa agcaccccgg cgtggacgac gtctatctgc
     4801 tggaactgga tcgtggttgg atcgtcgtgg atcgcagcgt cttcgacagc gttcagcccg
     4861 gcgacctgct tcgcaagccc cgctggagcc gccaactgca agtcggcagc aggacggtac
     4921 gcctgatatg gtccgccgat atgcggggga tgctcgtcgc aatgccgtgt gtgttcgtcg
     4981 tcgtcgcggc aaccatcgcg gctgtcaccc gccaagcgcg agcctcttag gactgcgaaa
     5041 aacggtcaat tcggcaggtc ccagacccaa ttggctttgc cgggtttagt caggcactct
     5101 ccagactttg aacttgccaa agccagagac tcccaccacg gtcgtcccgt cggctgaagc
     5161 tcgcaggcta acgatggtct cgcgctcgta attcggcgag gcacagattt ctcgtcccgt
     5221 ggcgatgtcg aaggcgacca gttgtccgcc gtcattgtgt cggcacaagt ggcgccacag
     5281 cggcaatgcc aaccgccacc gccaatcacc atcctcacga aagactgcca tgtcacagcg
     5341 acgtgagact gaccattggc cgcccttgac aaagaagtat gtgggaccgg ccacgctctg
     5401 tttagcgcct gtaggcagat cgaccaactc caggccgcag gcgacgcttc gaccatcggg
     5461 cgagagctcc ggcctggcga agcgcgatat cgatcgctca ggatagattg ccttaagagt
     5521 ttca
//
LOCUS       DASRJB010000694         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3867284, whole genome shotgun sequence.
ACCESSION   DASRJB010000694 DASRJB010000000
VERSION     DASRJB010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867284"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFB80_18450"
     CDS             <1..256
                     /locus_tag="VFB80_18450"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN35820.1"
                     /translation="ALPALMAALSVGDERLAESVRVALRQRGLRTAAGLTIEAQQATV
                     TLRGPARSFHEKQLVLHAVQHVPGVLRIVDEIDVVSGSGR"
     gene            complement(340..1653)
                     /locus_tag="VFB80_18455"
     CDS             complement(340..1653)
                     /locus_tag="VFB80_18455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35821.1"
                     /translation="MNDAHFQRGSLLYHQGRYADAIGELQLQLAAGESFLTHSLLALC
                     FSEQQKYPEAMEHVQRAIHLAPDKPLGHYALAQVLLQRNRPAEAQAAIEEAIRLDPSD
                     ADYYALLGGIALRQSRWRDALNAANQGLAIEPEHQFLINLRATALVKLGDRAAAAATM
                     GQALARRPDDPLTHANQGWALLHEGQPYKALEHFREALRLNPELEWARLGIVEALKAR
                     NFLYRAMLGYFLWMSRLPANVRIGLVGGALAANWIIGDLAVRSPHLAPFLWPVLYAYF
                     AFVLLSWLSYPLFNLLLRIDKFGRHALSADQRSGANVLAVCLGIFLLAAGRSIAARLG
                     WFGPTAVRQVELIDYCTQMCALLALPASAIYICDSGWPRQVMIAITAALAAAVALVSL
                     VVGFPLSDVSPLILAPLVMVIRALPFGMLASQIAANYLMGASPKK"
     gene            complement(1650..2990)
                     /locus_tag="VFB80_18460"
     CDS             complement(1650..2990)
                     /locus_tag="VFB80_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN35822.1"
                     /translation="MPGNDPLAALREALALSPDNVPLRQHLAESLLGSGQPEQAEAEF
                     KAALALAPESTGLKIGLARAFLAQQKASAALVILEAMVQRRDAPGLAHVLLARLQFGR
                     GEVPQAVAQYKLGVEKDPAAADLAFAGQLGIDSTAEDSEVVDGRMRAAWDEPGGAATE
                     IERPKINFESVGGMEALKEEIRLKIIHPLNHPELYKAYGKAIGGGMLMYGPPGCGKTH
                     LARATAGEIKAAFIAVGINDVLDMWIGNSERNLHALFEQARENKPCVLFFDEADALGA
                     KRSDMHHAGNRHLINQFLAEMDGIQSSNEGLLILAATNSPWHLDPAFRRPGRFDRVLF
                     VPPPDAAARASILRILVKGKPVQDLDLNLLAKKTDQFSGADLKAVVDLAIEGKLREAL
                     KAGVPKPLTTADLLGAAAQLKPTTKEWFATARNHALYSNQGGVYDDILKYLKLT"
     gene            complement(3094..3666)
                     /locus_tag="VFB80_18465"
     CDS             complement(3094..3666)
                     /locus_tag="VFB80_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN35823.1"
                     /translation="MASKKILMLVGDYVEDYEVMVPFQMLLMVGHDVQAVCPGKKAGQ
                     TVATAIHDFEGEQTYSEKRGHNFRLNASFDEIDEKTFDALVIPGGRAPEYLRLNPRVV
                     EIVRHFANMKKPIAAICHGVQLLSAAGVIRGRRVSAYPACRTEVDLAGGEFVPANSTF
                     DNAHTDGNLITAPAWPAHPAWMRQFLAALS"
     gene            complement(3712..4830)
                     /locus_tag="VFB80_18470"
     CDS             complement(3712..4830)
                     /locus_tag="VFB80_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZN35824.1"
                     /translation="MTTHCNTRGLLPAIALILAANLRAAELPWIKVSGDQKSFVTEPG
                     GQPFVPWGFNYDHDETKAARLIEDYWEGEWPKVEADFREMKELGANVVRIHLQFGKFM
                     EAADKPSERSLAQLARLVKLAEATGLYLDLTGLACYHKKDVPAWYDALEEGERWAAQA
                     AFWKAVAATCKDSPAIFCYDLMNEPVVPGRVGRAADWLGPPLGDKHFVQYITREAKGR
                     ERHDIARQWIEVLVKAIRAVDRRHLITVGLVPWSLDKPGLTSGFVPDKIAGELDFVAV
                     HVYPEAGKLDEALETLAGFNIGKPVVVEEMFCLKCSADELGRFIDQSREKKTAAGWIG
                     FYWGRTPEELRGGATLAEAFTLSWLELFQKKRAELFTK"
     gene            4901..>5923
                     /locus_tag="VFB80_18475"
     CDS             4901..>5923
                     /locus_tag="VFB80_18475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN35825.1"
                     /translation="MFRRLLALAIALSAALLCVRTADAQKLNVLLIASDDLRPELGCY
                     GNKLIDTPNFDRLAGWGVRFDRANCQYPLCNPSRSSLLTGRHPNTTGVLDNRTYFRDA
                     HPDFVSLPQHFKSHGYVTARTGKIFHGGIDDTENWTIGGEQRVDQKTRPGQNPMLSDR
                     IVKLEGDGESHGDYKSATRAIALLDELKDKPFFLAVGFSKPHSPPTAPAMLFDLYDAA
                     RMPLPPDFAALPAAPEGFPEASIPKRSGDLFIGREASREAAQEVIEAYYASTTFVDRQ
                     IGRVLDRVERLGLRDKTVIVLFGDHGYHLGEKGKWSKHGSVFEVAARVPMIVCAPGGA
                     AGKSSDR"
BASE COUNT         1016 a         1942 c         1932 g         1033 t
ORIGIN      
        1 tgctcttccc gcactgatgg cggctctgtc cgtaggcgat gagcgattgg ccgagtcggt
       61 aagggttgcc ctgcgtcagc gcgggctgcg gaccgcggcg gggctgacca ttgaagctca
      121 acaggctacc gtcacgctcc gcggaccggc gcgatcgttc catgagaagc agctcgtgct
      181 ccatgcggtg cagcacgtgc cgggcgtact gcggatcgtg gacgagatcg acgttgtgtc
      241 cggcagcggg cgctgaatcg ccgccttcgg cgacatcctg tcgcctaacg cgtcgtcaag
      301 cgacgctctg tcgccctcaa ggccgcggtg acggcgtggt tactttttcg gcgaagcgcc
      361 catcaagtag ttcgcggcga tttgcgaggc gagcatgccg aagggaaggg cgcgaatgac
      421 cattaccagt ggcgccagaa tcagcggaga gacgtccgac aacggaaagc cgacgaccag
      481 cgagacgagg gcgacggccg cggcgagggc ggcggtaatc gcgatcatca cttgccgtgg
      541 ccagccggag tcgcaaatgt agatggccga agcgggcagg gccaacaggg cgcacatttg
      601 cgtgcaatag tcgatcagtt cgacctgccg gacggctgtg gggccgaacc agcctagccg
      661 ggcagcgatc gaccggcccg cggcgagcaa aaagatgccg aggcagaccg ccagcacgtt
      721 cgccccgctc cgctggtcgg ccgacaaggc gtgccggccg aacttatcaa tccgcagcag
      781 caaattgaat aacggatacg acagccacga cagcagcacg aatgcaaagt aggcgtagag
      841 caccggccac aggaatggag cgaggtgcgg cgaacggacg gccagatcgc caatgatcca
      901 gttggcggcc agcgccccac ctaccaggcc gatacgcacg tttgccggca gccgggacat
      961 ccacaggaaa tagcccagca tcgctcggta caggaagttg cgggccttga gggcctcgac
     1021 gatgccaagg cgggcccatt ccagctcggg gttcagccgc agggcctcgc gaaagtgctc
     1081 cagcgctttg tagggctggc cttcgtggag cagggcccag ccttggttgg cgtgcgtcag
     1141 cgggtcgtcg gggcggcggg ccagcgcctg gcccatggtc gcggctgcgg cggcccggtc
     1201 ccccagcttg acgagggccg tcgcccgcag gttgatcaga aactggtgtt ccggctcgat
     1261 cgcgagtccc tgattggccg cgttcagggc gtcgcgccag cggctttgcc gcaatgcgat
     1321 gccgccgagc aaggcgtaat agtcggcatc ggagggatca agccggatcg cctcctcgat
     1381 cgcggcctgg gcctcggcgg ggcggttacg ctgcagcagc acctgcgcca gggcatagtg
     1441 tcccagcggc ttatcgggcg ctagatggat cgcgcgctgg acgtgctcca tcgcctccgg
     1501 atatttctgc tgctccgaga aacagagcgc cagcaggctg tgggtgagaa acgactcgcc
     1561 cgctgccagt tggagctgca actcgccgat cgcgtccgcg tatcgcccct ggtgatagag
     1621 cagcgacccg cgctggaagt gggcgtcgtt cacgtcagct tgaggtactt gagaatgtcg
     1681 tcgtagacgc cgccctggtt ggagtacagc gcgtggttgc gggcggtggc gaaccattcc
     1741 ttggtggtcg gtttcagctg cgcggccgcg cccagcaggt cggccgtggt cagcggcttg
     1801 ggcacgccgg ccttgagcgc ctcgcggagc ttgccttcga tcgccagatc gacgacggcc
     1861 ttcagatccg cgccggagaa ctggtcggtc ttcttcgcga gcaagttcag gtcgaggtcc
     1921 tgcaccggct tgcccttgac gagaatccgc aggatgctgg cccgcgcggc ggcgtcaggg
     1981 ggcggaacga acagcacgcg gtcaaaccgg ccggggcggc ggaacgcagg atcgaggtgc
     2041 cagggagagt tcgtcgccgc caggatcagc agaccctcgt tcgacgactg aatgccgtcc
     2101 atctcggcca gaaactggtt gatcaggtgg cggttgccgg catgatgcat gtccgaccgc
     2161 ttggccccca gggcgtccgc ctcgtcgaag aacagtacgc agggcttgtt ctcgcgggcc
     2221 tgctcgaaga gggcatgcag gtttcgctcg ctgttgccga tccacatgtc gagcacgtcg
     2281 ttgatgccga cggcgatgaa agcggccttg atctcgccgg ccgttgcccg ggccaggtgc
     2341 gtcttgccgc agcccggcgg accgtacatc agcatcccgc cgccgatcgc cttgccgtag
     2401 gccttgtaga gctcgggatg gttgagcggg tggatgatct tgaggcggat ttcctccttg
     2461 agggcctcca tgccgccgac gctttcgaag ttgatcttgg gccgctcgat ctcggtcgcc
     2521 gcgccgcccg gctcgtccca ggcggcccgc atgcggccat cgaccacttc ggagtcttcc
     2581 gcggtgctgt cgatgccgag ctgccccgcg aacgcgagat ccgcggccgc ggggtccttt
     2641 tcgactccga gcttgtactg cgcgacagcc tgagggactt cgccgcggcc gaactgcagc
     2701 cgggccagca gcacgtgggc cagaccgggc gcatcgcggc gctgcaccat ggcctccaga
     2761 atgaccagcg ccgccgaggc cttctgctgc gcgagaaatg cccgggccag cccgatcttg
     2821 aggcccgtgc tttccggcgc gagtgccagc gccgccttga attcggcctc cgcctgctcc
     2881 ggctgcccgc tccccagcag cgactcggcc aggtgctggc gtagcgggac attgtcgggc
     2941 gagagtgcca gcgcctcgcg cagcgctgcc aaaggatcgt ttccgggcac ggcggcctca
     3001 agcggcggat gaatcgggaa gggagaattg agtttgcggg cgcacttgca gggcgtcaat
     3061 actcgtaggc gacgctgcca gcgtcgtcaa cggctaactc agcgcggcca ggaattgccg
     3121 catccaggcc ggatgagcgg gccaggccgg ggcggtgatc aggttgccgt cggtgtgggc
     3181 gttgtcgaaa gtgctattgg cggggacgaa ttcgccgccg gccaaatcca cctcggtgcg
     3241 gcaagccgga taggcgctca cgcggcggcc acggatcacg cccgccgctg agagcaactg
     3301 caccccgtgg cagatcgcgg cgatcggctt cttcatgttg gcaaaatgcc gcacgatctc
     3361 gaccacgcgg ggattcagcc gcagatactc cggcgcgcgg ccgccgggaa tgaccagggc
     3421 gtcgaaagtc ttctcgtcga tctcgtcgaa cgaggcgttg aggcggaagt tgtgcccgcg
     3481 tttttcgcta tacgtctgtt cgccttcgaa atcgtgaatg gccgtggcca cagtctgccc
     3541 ggccttcttg ccgggacaaa ccgcctgcac gtcgtggccg accatcagca gcatctggaa
     3601 cgggaccatg acttcgtagt cttccacata atcgccgacc agcatcagga tttttttgct
     3661 tgccatatca cattctcttc gtgctctttg tgaccttcgt gtttaactgc cttacttcgt
     3721 aaacaactcc gcgcgcttct tctggaacag ctccagccac gacaacgtga atgcctcggc
     3781 cagcgtcgcg ccgccgcgga gttcctccgg cgtgcggccc cagtaaaagc caatccagcc
     3841 ggcggccgtc tttttctcgc gcgactggtc gatgaaccgc cccagttcgt cggccgagca
     3901 tttgaggcag aacatctctt cgaccacgac cggcttgccg atattgaacc ccgcgagcgt
     3961 ttccagcgct tcatcgagct tgcccgcctc cggatagacg tgtaccgcga cgaagtcgag
     4021 ctcgccggcg atcttgtccg gcacaaagcc cgatgtaagc cccggcttgt ccaggctcca
     4081 gggcacgagg ccaaccgtga tcaggtgccg ccgatcgacc gcgcggatcg ccttcacgag
     4141 cacctcgatc cactgcctgg caatatcgtg ccgctcgcgg cccttcgcct cccgcgtgat
     4201 atattgcaca aaatgcttgt cgcccagcgg cggcccgagc caatcggcgg cccggccgac
     4261 gcgccccggc acgaccggct cgttcatcag gtcgtagcag aaaatggccg ggctgtcctt
     4321 gcacgtggcg gcaacggcct tccagaatgc cgcctgcgcc gcccagcgct cgccctcctc
     4381 cagcgcgtcg taccacgcgg gcacgtcttt cttgtggtag caagccaggc ccgtcagatc
     4441 gaggtacagc ccggtcgcct cggccagctt gaccagccgg gccaactgcg ccagcgaacg
     4501 ctcgctcggc ttgtccgccg cctccatgaa tttgccgaac tgcaggtgga tacggacgac
     4561 gttggccccc agctccttca tctcgcgaaa atcggcctcg actttgggcc actcgccctc
     4621 ccaatagtcc tcgatcagcc gagcggcctt ggtctcgtcg tgatcgtaat tgaaacccca
     4681 cggcacgaac ggctgcccgc ccggctcggt cacgaacgac ttctgatcgc cgctgacctt
     4741 gatccacggc agctcggcgg cccgcaaatt cgccgcaagg atcagggcaa tcgccggcag
     4801 cagaccgcga gtgttgcaat gcgttgtcat cgcacctagc ttagcgaatg caactactcc
     4861 tatcagcccg tgctctgcag ttgtcttcgg tgaggcccat atgttccgac gactcctcgc
     4921 gctcgcaatt gccctatcgg cggcattact ctgcgtgcga acggccgacg cccagaagct
     4981 caacgtgctg ctgatcgcca gcgacgatct gcggccggag ctgggctgct acggcaacaa
     5041 gctgatcgat acgccgaatt tcgaccggct ggccggctgg ggagtgcggt tcgaccgggc
     5101 caactgtcag tatccgctct gcaatccgtc gcggtcgagc ctgctgaccg ggcggcatcc
     5161 gaatacgacg ggcgtgctgg acaaccgcac ttacttccgc gacgcccacc cggatttcgt
     5221 ctcgctgccg cagcatttca agagccacgg ctacgtcacg gcccgcaccg gcaagatctt
     5281 tcacggcggc atcgacgaca cggaaaactg gacgatcggc ggcgagcagc gggtcgatca
     5341 aaagacgcgg ccggggcaga acccgatgct ctcggaccgg atcgtcaagc tggagggcga
     5401 cggcgagtcg cacggtgatt acaaatcggc cacgcgggcc atcgccctgc tggacgagct
     5461 gaaggacaag ccgttcttcc tggccgtcgg cttcagcaag ccgcacagcc cgcccacggc
     5521 cccggcgatg ctcttcgact tgtacgacgc ggccaggatg ccgctgccgc cggattttgc
     5581 cgcactgccc gccgcgcccg agggatttcc cgaggcctcg attcccaagc ggagcggcga
     5641 cctgttcatc ggccgcgagg cttcgcgcga ggcggctcag gaggtgatcg aagcatatta
     5701 cgcctcgacg acgttcgtcg atcgacaaat cggccgcgtg ctcgaccgcg tcgagcggct
     5761 gggcctccgc gacaagacgg tgatcgtcct gttcggcgac cacggctatc acctgggcga
     5821 gaaaggcaaa tggtccaaac acggctcggt gtttgaagtg gcggcgcggg tgccgatgat
     5881 cgtgtgcgct cctggcggtg cggcaggcaa gagctcagat cgg
//
LOCUS       DASRJB010000695         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_489435, whole genome shotgun sequence.
ACCESSION   DASRJB010000695 DASRJB010000000
VERSION     DASRJB010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489435"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3018
                     /locus_tag="VFB80_18480"
     CDS             <1..>3018
                     /locus_tag="VFB80_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZN35826.1"
                     /translation="DGQILAPLDAGDALGKLTALRDAGIESLAICLLNSYANPSHEEQ
                     LETLARQAGFAEISLSSRVAPLMKIVSRGDTTVVDAYLNPLLRQYVERLRSALPGSDV
                     RLMTSSGGLVSGERFSGKDSILSGPAGGVVGFSRAAQAAGFAKAIGFDMGGTSTDVAR
                     FDGRFEYEHETEKAGVRIVAPMLAIETVAAGGGSICRFDGVKLVVGPESAGADPGPAC
                     YGRGGPLTVTDCNLFLGRLIEEHFPFSLSHAAVEQRLAQIAGEVAAAGKPLSATELAE
                     GFLAIANANMAQTIRSISLAKGYQPADFVLVAFGSAGPQHACAISRQLGIRRILIHPD
                     AGVLSALGIGLADVVRHEVAGIYEPLLADSLSRTESKFDELAAAAVAHVEAEGVPRER
                     IELRRSLDLQYRGVDAYITVAEPSDGDYAQEFAAVHQQLYGYLHEGRPLEIVAARVEA
                     VGHTAAQAPNLFRNPSSQPPQIVLAPSGRFPVYRREDLPAGTKLDGPALVVEPLTTTV
                     MEAGWRGEVLPGGELVLSDEAGNTSTAAGTSTQADPIQLELFNGRLTAIATQMGITLR
                     NTAGSVNVKERLDFSCAIFTADGDLVVNAPHIPVHLGAMSQTVRQVFADNPNMRPGDV
                     FVTNDPYRGGSHLPDVTVVTPVFDDALRFFVASRAHHAEIGGITPGSMPPFSKSLAEE
                     GVLLRNLKLADGGKLRLDVLRQALLSGPYPSRAVEDNLADITAQITANHQGVRDLSAF
                     IAEQSWPVVEAYMRHIQDAAERKMRAALAKLPPGRREFVDHLDDGSPIAVAVSIDGDR
                     ATIDFSGTGPVLPGNLNANRAIVSAAIMYCLRLLIGEEIPLNQGLLKPVELILPECLL
                     NPPARERPEECAAIACGNVETSQRVVDVLLGTFQLAAASQGTMNNLLFGDAAFGYYET
                     ICGGSGATVECHGADAVHTHMTNTRLTDPEVLERRFPARLLEFSIRRGSGGRGKHRGG
                     DGVVRRIEFLRELTVSILSQRRGPFLPYGMEGG"
BASE COUNT          536 a          940 c         1010 g          532 t
ORIGIN      
        1 cgacggccag atccttgcgc cgctggatgc aggggatgcg ctggggaaat taacggcgct
       61 gcgcgacgcg ggaattgagt ccttggcgat ttgcctgctt aatagctacg cgaatccgtc
      121 gcacgaagag cagctcgaaa cactcgcccg tcaggccgga ttcgcggaaa tcagcctgtc
      181 cagtcgcgtc gcgccgctga tgaaaatcgt ctcccgcggc gacacgaccg tcgtcgatgc
      241 ctatctcaat ccgctcctgc ggcaatatgt ggagcgcctg cggtcggcac tccccggcag
      301 cgacgtgcgc ctgatgacca gcagcggggg cctggtgtcc ggcgagcggt tcagtggcaa
      361 ggacagcatc ctctccggcc ccgccggcgg cgtagtcggc ttttctcgag cggcgcaagc
      421 cgcgggattc gccaaggcga tcggcttcga catgggaggc acgagtactg atgtcgctcg
      481 attcgacggc cgcttcgagt acgagcacga gaccgagaag gcaggcgtgc ggatcgtcgc
      541 gccaatgctg gccatcgaga cggttgcggc tggcggcgga tcgatctgcc gcttcgacgg
      601 cgtaaaactg gtcgtgggcc cggaaagtgc gggcgccgat cccgggcctg cctgctatgg
      661 ccgcggcggc ccactgaccg tcaccgactg caatctgttc ctagggcggc tcatcgagga
      721 acattttccc ttttccctgt cccatgcggc agtcgagcag cggctggccc agattgccgg
      781 cgaggttgcc gcggcgggaa agccgttgtc ggcgaccgag ctggcggagg gttttctggc
      841 gatcgccaac gccaacatgg cgcagacgat ccgctccatc tcgctggcca agggatacca
      901 gccggccgac tttgtcctcg tcgcctttgg atcggccggg ccgcagcacg cctgtgcaat
      961 ctcccgccag ctcggcattc gtcgcatctt gatccaccct gatgccggcg tgctcagcgc
     1021 gctagggatt ggcctggcgg acgtggtgcg gcacgaagtg gcaggcatct acgagccgct
     1081 gttggcggat agcctgtcgc ggacggaatc gaagttcgac gaattggccg cggcagccgt
     1141 tgcgcacgtc gaagcggagg gagtgccgcg ggagcgaatc gaacttcggc ggtcgctcga
     1201 tttgcagtac cggggcgtcg atgcgtacat cacggtcgcc gagccgtctg acggcgatta
     1261 cgcccaggag tttgccgcgg tgcatcagca gctctatggc tacctgcacg aggggcgacc
     1321 gctggaaatt gtggcggcgc gggtggaggc agtgggccac accgcagcac aggctcccaa
     1381 cctgttcaga aacccttcca gccaaccgcc gcagatcgtg ttggcgccaa gcgggcgctt
     1441 tcccgtttat cgacgcgaag atttgcccgc cggcacaaag ctcgacggtc ccgcactcgt
     1501 cgtggagccg ctgacgacca ccgtgatgga ggcgggctgg cgcggcgaag tgcttcccgg
     1561 cggcgaattg gtgctcagcg acgaggcggg caatacttca accgccgcgg ggacatcaac
     1621 gcaggcagat cccattcaac tggaactatt taacggccgg ctgaccgcca tcgcaaccca
     1681 gatgggaatc acgctcagga acacggccgg cagcgtcaac gtgaaggagc gacttgactt
     1741 ctcgtgcgcg atcttcaccg ccgacggcga tctggtggtc aacgcgccgc atatccccgt
     1801 gcatctcggg gcgatgagcc agacggtacg gcaagtgttc gccgacaatc cgaacatgcg
     1861 gccaggagat gtgttcgtca ccaacgaccc gtatcggggc ggctcgcact tgccggatgt
     1921 aaccgttgtc acgccggtgt ttgacgatgc actccgtttt tttgtcgcca gccgcgcgca
     1981 tcacgcggag atcggcggca tcacacccgg ctcgatgccg ccgttttcca aaagcctggc
     2041 ggaagaggga gttttgctgc ggaatctcaa gctggccgac ggcggcaagc tgcggctcga
     2101 tgtactgcgg caggccctcc tgtcgggccc gtatcccagc cgcgcggtcg aggacaacct
     2161 ggccgacatc acggcgcaga tcacagccaa tcatcaaggg gtccgcgact tgtcggcatt
     2221 catcgccgag caatcgtggc cggttgtgga agcgtacatg cggcatattc aggatgccgc
     2281 ggaacggaag atgcgagcag cactggcgaa gctgccgccg ggacgccgcg agtttgtcga
     2341 tcatctcgat gacggctcgc cgattgccgt ggcggtgagc atcgatgggg atcgcgcgac
     2401 catcgacttt tcaggaacag gccccgtcct gcccggcaac ctgaacgcca atcgggcgat
     2461 cgtctcggcg gcaatcatgt attgcctgcg gctgctgatt ggcgaggaga ttccgctcaa
     2521 tcaggggctg ctcaagccgg tggaacttat tctgcccgag tgcctgctga acccgccggc
     2581 gcgcgagcga cccgaggaat gtgcggctat cgcctgcggc aacgtggaaa cgagccagcg
     2641 cgtggtcgat gtgctcttgg gaacattcca gttggcggcg gccagccagg ggacgatgaa
     2701 caaccttctc tttggcgacg cggcattcgg ctactacgag acgatctgcg gcggcagcgg
     2761 cgcgacggtg gaatgccacg gggccgatgc cgttcacacg cacatgacca acacgcggct
     2821 gaccgatcca gaggtgctgg agcggcgctt tccggcgcgg ctgctggagt tttcaatccg
     2881 ccgaggctcg ggcggacgcg gcaaacaccg cgggggcgac ggcgtcgtgc gccgaattga
     2941 atttctcagg gagctaacgg tctcaattct ctcacagcgc cggggaccct ttctgccgta
     3001 tggcatggag ggcggcga
//
LOCUS       DASRJB010000696         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2460155, whole genome shotgun sequence.
ACCESSION   DASRJB010000696 DASRJB010000000
VERSION     DASRJB010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460155"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="lysS"
                     /locus_tag="VFB80_18485"
     CDS             <1..1388
                     /gene="lysS"
                     /locus_tag="VFB80_18485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZN35827.1"
                     /translation="EPELDFRGWLQDQGKGEWSGPKVRAAGRIVLSRDAGKLRFLNIQ
                     DWTGNLQLLVGKAQVGDESWALAECFDLGDIIGVDGKLAYTKTGELTIFAEKLHFLSK
                     SLAPPPDKHSGLADPELRQRMRYLDLAYGDGVLDRFRNRTKIVQSIRGTLNARGFCEI
                     EGPTLHTIAGGAAARPFKTHHNTLDIGLVLRIALELHLKRLLVGGMERVYELGRVYRN
                     EGISPRHNPEFTMLEVYQAFGDYGSMMDLTEAIIADAITAIDGNFKRSWGNNAARPEG
                     DTVIDFTPPFDRKTYDELFREETGIDPQDQAAVKGLADRIGFDTAGKHPDVIKSEVFE
                     DQVEDALTGPIFVIDYPASICPLTKRKRSNPAVAERFELFVQGMEVANAYTELNDPDL
                     QEQLFQTQLAGLAEENSMAKMDHDFIRALRHGMPPAGGLGIGIDRLVMLLTNSPSIRD
                     VILFPLLRPEA"
     gene            complement(1402..2355)
                     /locus_tag="VFB80_18490"
     CDS             complement(1402..2355)
                     /locus_tag="VFB80_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35828.1"
                     /translation="MATSKRAFTLVELLVVIAIIGVLVALLLPAVQSAREAARRMQCG
                     NNLKQITLGVHNYADTYRGALPVGEYSWGWGTWLVGMLPYIEQKNLFDLYQGYGGINA
                     AGGIDTNFAYSATINLPVTRSTLNTYTCPSDNKVRPRGVITFHNYLANHGNTTYGRQA
                     TFGTMSNGQPNRFGGAPFINVGNSNTIPQVIRLAEIVDGLSNTLAFSETIKGQPNGQD
                     LRGFAWWSSGAHFETNLMPNSNQPDVVEQDPYCVNQRPNPPCVGPTSANPQNIAARSR
                     HPSGVQVSLCDGSVRFISNNIFLDNWRAISTTGGGETVSDF"
     gene            complement(2411..2851)
                     /locus_tag="VFB80_18495"
     CDS             complement(2411..2851)
                     /locus_tag="VFB80_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35829.1"
                     /translation="MAIQRIAIAPVAGAALWLLCGCGGDSLGRHAISGSVSVDGTPLA
                     KGNISFQPTDNQPTSSGAVVTGGKFSIPRESGLVVGKYRVVVNAAVPGTEGKAIPADA
                     QPGDPPAPAIEMIPPDWNVSSQHTIEVKREGPFDFPFEISTKGK"
     gene            complement(2965..3915)
                     /locus_tag="VFB80_18500"
     CDS             complement(2965..3915)
                     /locus_tag="VFB80_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35830.1"
                     /translation="MNERWLVLMILLAAAGCRSTHWLRRGGGDPPPIAFSALPSPEEA
                     VTAVNANTQRVQSLQTQGATVSIPGAPSIGAEIQVERPRRLRFRAGTQLLGPELDLGT
                     NDELFWLWAARMPGSAVYFARHEQFASSPARQMLSIEPAWLIEALGLVEIDPASVIEG
                     PHADGKDRVKLKTTLPSSGMPYTRLVILHSKYAWVLEQHVYDEREQLIASARASGHEF
                     YSLDGVSLPKRIEVNMPLGQMRFQLDVTRWGINQPLADGPALFELPRAALSNYPFLDL
                     ADPNFIPPGGTTPVEQPAPRTSHVPADHYRQRLRGFSQWR"
     gene            complement(3988..4476)
                     /locus_tag="VFB80_18505"
     CDS             complement(3988..4476)
                     /locus_tag="VFB80_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35831.1"
                     /translation="MIDYDIQRCSRRCAASDRELKNGEECYSVLIEQAGGIVRRDYSA
                     EGWPGPPDNAIGWWKSTVIDPNAGRMSWAPNDVMLHYFERLADDPAAEDARYVLALLL
                     VRRRVLRVDGNETDAGGRPLLVLYCGRNEAQYRVAEVAPTPERTAAIQQQLADLLQTN
                     GS"
     gene            4585..>5668
                     /locus_tag="VFB80_18510"
     CDS             4585..>5668
                     /locus_tag="VFB80_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN35832.1"
                     /translation="MSLAAIPELRALDTRRHLVRIPHEEDVPLTERVRLLLDAPEFRR
                     LARVSQLGLVSLVYPGACHSRLEHSLGVYRNALVYLKQLAGDERFAATVRPQDAEALL
                     CAALLHDLGHWPFCHAIEDMALPGVPTHELFANSFLLEGEIADTLRDEWSVQPRDVTA
                     LLSEKPTGTRQRLLKSLLSGPIDIDKMDYLGRDSLHAGVPYGRNYDQQRLIGSLCLNQ
                     AGDGLAISHKGRTAAEMMVFARYVMFSEVYWHHAVRSATAMLQRAFFLLHGQLDLDQL
                     FRQSEPAFIDQLLAVARDSVAGTRDGVASYRTPADELLSGLFGPTRRLYKRLAEFSHF
                     QHREWYDRLARQPYPWLVRASESFAAA"
BASE COUNT          991 a         1867 c         1838 g          972 t
ORIGIN      
        1 aggagcccga gctcgacttc cgcggctggc tgcaagacca gggcaaggga gagtggtcgg
       61 gccccaaggt gcgggcggcg gggcggatcg tgctatcgcg cgacgcgggg aagctgcggt
      121 tcctcaacat ccaggactgg accggcaacc tccagttgct ggtcggcaag gcgcaggtcg
      181 gcgatgagag ctgggctctg gccgagtgct ttgacctggg cgacatcatt ggcgtggacg
      241 gcaagctggc ctatacgaag accggcgagt tgacgatctt cgccgagaag ttgcacttcc
      301 tgagcaagag cctcgccccg ccccccgaca agcattcggg cctggccgac ccggagctgc
      361 ggcagcggat gcggtatctc gacctggcgt acggcgacgg agtgctcgac cgcttccgca
      421 accgcaccaa gatcgtgcag tcgatccgcg gcacgctaaa cgcccgcggc ttctgcgaga
      481 tcgaaggccc gacgctgcac accatcgccg gcggagcggc ggcccggccc ttcaagaccc
      541 accacaacac gctcgacatc ggcctggtgc tgcggatcgc cctggagctg cacctcaagc
      601 ggctgctggt cggcggcatg gagcgggtct atgagctggg ccgcgtctat cgcaacgaag
      661 gcatcagccc gcggcacaat cccgagttca cgatgctcga ggtctatcag gccttcggcg
      721 actacggctc gatgatggac ctgaccgagg cgatcatcgc cgatgcaatc accgccatcg
      781 acggtaactt caagcggtcc tggggcaata atgctgcgcg cccagagggc gacaccgtca
      841 tcgacttcac gccgccgttc gaccgcaaga cctacgacga gctgttccgc gaagaaaccg
      901 gcatcgatcc gcaggaccag gctgccgtga agggcctcgc cgaccggatc ggtttcgaca
      961 cggccggcaa gcatcccgac gtgatcaaga gcgaggtctt cgaggatcag gtcgaggacg
     1021 ccctcaccgg cccgatcttc gtcatcgatt atcccgcgag catttgccca ctcaccaagc
     1081 ggaagcggtc gaaccccgcg gtggccgagc ggttcgagct attcgtgcag ggcatggaag
     1141 tcgccaacgc ctataccgag ctgaacgatc ccgacctgca ggaacagctc ttccagacgc
     1201 agctcgccgg cctcgccgag gaaaactcga tggccaagat ggaccacgac ttcatccgcg
     1261 ccttgaggca cggcatgccg ccggcgggcg gcctggggat cggcatcgac cggctggtca
     1321 tgctgctcac caacagcccc tcgatccgcg acgtgatcct ctttccgctg ctgcggcccg
     1381 aggcctgatg gcggcacgaa atcaaaaatc gctgaccgtt tccccgccgc cggtggtgct
     1441 gatcgcgcgc cagttgtcga gaaatatgtt gttggaaatg aaacggacgg agccgtcaca
     1501 gagcgagacc tggacgccgc tgggatggcg gctgcgggcg gcgatgttct gcggattggc
     1561 ggacgtcggc ccaacgcagg gcggattggg gcgctggttg acgcagtagg gatcctgctc
     1621 gaccacgtcc ggctggttgg aattcggcat cagattggtc tcgaagtgtg cgccgctgct
     1681 ccaccaggcg aaaccgcgca gatcctgccc gttcggctgg cccttgatcg tttcggaaaa
     1741 tgccagcgtg ttgctcaggc cgtccacaat ctccgccagg cgaatgacct gcggaattgt
     1801 gttggaatta cccacgttga taaacggcgc gcctccgaac cggtttggct gcccgttgct
     1861 catcgtgccg aaagtcgcct gccgcccgta ggtcgtgttg ccgtggttgg ccagataatt
     1921 gtggaaggta atcacgccgc gaggtcgcac cttgttgtcg ctcgggcagg tgtaggtgtt
     1981 gagcgttgag cgcgtgacag gcaagttgat ggtcgcgctg taggcgaagt tcgtatcgat
     2041 gcccccggcg gcattgatgc cgccgtagcc ctgatagagg tcgaagaggt ttttctgctc
     2101 gatgtacgga agcattccga cgagccaggt tccccagccc cacgaatact cgccgacggg
     2161 aagcgcgccg cggtaggtgt cggcatagtt gtgtacgccc aaggtgatct gtttgaggtt
     2221 gttcccgcac tgcatgcggc gggctgcttc gcgggccgac tgcaccgccg gcaagagaag
     2281 agcgaccagc actccgatga tggcgatgac aaccagcaac tcgaccagcg tgaacgctcg
     2341 tttcgaagta gccatggcag cctccggaag cgggtcggtt tgtgaacgag acgacttcaa
     2401 caggtccgct ctacttgcct ttcgtcgaaa tctcaaaggg aaagtcgaac ggaccttcgc
     2461 gtttgacctc aatggtgtgc tggctcgaca cattccagtc gggcggaatc atctcgatcg
     2521 ccggagccgg cggatcgccc ggctgggcat cggcgggaat cgcctttccc tcggtgcccg
     2581 gcacagcggc attgacgacg acccggtact tgccgaccac gagaccgctc tcgcgcggaa
     2641 tcgagaactt gccgcccgtg acaaccgcgc cgctcgacgt cggctgattg tccgtgggtt
     2701 gaaagctgat gttgcctttc gcgagcggcg tgccatcaac ggacaccgag cctgagatgg
     2761 catgccggcc caaggagtcg ccgccgcatc cgcacaggag ccagagggcc gcccccgcaa
     2821 cgggcgcaat cgcaatgcgc tgaatagcca ttgctgcctc gacaaagccg cctgaccgaa
     2881 aggtcgtgcc gccccccaac gtccgttagg cgcaatctag gcttcgccac tggaaaggtc
     2941 aacgagaaca gattccgcgg agccctaccg ccactgcgag aagccgcgga gccgctggcg
     3001 gtagtgatcg gcgggaacgt gcgaagtccg cggcgccggt tgctcgacgg gcgtcgtgcc
     3061 gccgggaggg atgaagttcg gatcggccag atcgaggaac ggatagttcg agagagcggc
     3121 ccgcggcagc tcgaacagcg ctgggccatc ggccagcggc tgattgatgc cccagcgggt
     3181 cacgtcgagc tgaaaccgca tctgccccag cggcatgttg acctcgatcc gcttgggcag
     3241 cgaaacgccg tcgagcgaat agaactcgtg gccgctggcg cgggcggacg cgatcagctg
     3301 ctcccgctcg tcatacacgt gctgctccag aacccaggcg tatttgctgt gcaggattac
     3361 cagccgcgtg tagggcattc cggacgatgg cagcgtcgtt ttgagcttga cgcggtcctt
     3421 gccgtcggcg tgcgggcctt cgatcacgct ggccgggtcg atttcgacca ggcccaaagc
     3481 ctcgatcagc caggccggct cgatggagag catctgccgc gccgggctgc tggcgaactg
     3541 ctcgtggcgg gcgaagtaaa ccgcggagcc gggcatccgc gcggcccaca gccagaacag
     3601 ctcgtcgttc gtccccagat cgagctccgg cccaagcaac tgcgttcccg cgcggaaccg
     3661 cagccggcgg ggacgttcga cctggatttc cgcgccgatc gatggtgcgc cggggatcga
     3721 gacggtcgcg ccctgcgtct ggagcgactg cacccgctgc gtgttcgcgt tgaccgccgt
     3781 cacggcctct tccgggctgg gcagcgccga gaacgcgatc ggcggcggat cgcctccgcc
     3841 ccgccgcagc cagtgcgtcg agcggcagcc ggccgcggcg agcaggatca tcagcaccag
     3901 ccagcgctcg ttcaccgttt catccttgaa gccgtcaatc gttggcctca cgctccgcgt
     3961 gaggattcgt ctcgcggagc gagacgacta cgatccattc gtctgcaaca aatccgccag
     4021 ctgctgctgg atggccgcgg tccgctcggg cgtgggcgcc acctcggcca cacggtactg
     4081 ggcttcgttg cggccacagt agagaacgag cagcggccgt ccgccggcgt ccgtttcgtt
     4141 cccatcgacc cgcagcacgc gccgccgcac gagcagcagc gcgagcacat agcgggcgtc
     4201 ttcggccgcc gggtcgtccg ccagccgctc gaagtaatgg agcattacat cgttcggagc
     4261 ccacgacatg cggccggcgt tcggatcgat cacggtcgat ttccaccagc cgatcgcgtt
     4321 atcgggcggc cccggccagc cttccgcgga atagtcgcgg cgcacaatgc cgccggcctg
     4381 ctcgatcagc accgagtagc actcctcgcc gttcttcagc tcgcgatcgc tcgccgcgca
     4441 ccgccggctg caccgctgaa tgtcgtagtc gatcatagaa gtgggccgcg gatagtacgc
     4501 cgcaccccat tttgacacaa tcgtagaaag ccggaagcaa cgccagctag cattcccact
     4561 ctgcattctg cattctgaat tccgatgtct ctggccgcta tccccgaact ccgcgccctc
     4621 gacacccgcc ggcacctcgt gcggattccg cacgaggaag acgtaccgct gaccgagcgc
     4681 gtgcggctgc tgctcgacgc gccggagttt cgccgcctgg cccgcgtcag ccagctcggc
     4741 ctggtttcgc tcgtctatcc cggtgcctgc cactcgcggc tggagcattc tctgggcgtc
     4801 tatcgcaacg cgctggtcta cttgaagcag ctcgcgggcg acgagcggtt cgcggcgacg
     4861 gtgaggccgc aggacgcgga agccctgctc tgtgcggcgc tgctgcacga cttgggccac
     4921 tggccgttct gccatgcgat cgaggacatg gccctgcccg gcgtgccgac gcacgagctg
     4981 tttgccaaca gttttctgct ggagggcgag atcgccgaca cgcttcgaga tgagtggagt
     5041 gtgcagccgc gggatgtcac cgcgctgctc tcggagaagc cgacggggac gcggcagcgc
     5101 ctgctcaaga gcctgctgag cgggccgatc gacatcgaca agatggacta cctgggccgc
     5161 gacagcctcc acgcgggcgt gccctatggc cgcaactacg accagcagcg gctgatcggc
     5221 agtctttgcc tcaaccaggc gggcgacggc ctggcgatca gccacaaggg ccgcacggcc
     5281 gccgagatga tggtcttcgc ccgctacgtg atgttcagcg aggtctattg gcatcacgcc
     5341 gtccgctcgg ccacggccat gctgcagcgg gccttcttcc tgctgcacgg gcagctcgat
     5401 ctcgaccagc tcttccggca gagcgagccg gcgtttatcg atcaattgct ggcagtagcc
     5461 cgcgactccg tcgcgggaac tcgcgacgga gtcgcgagct acaggacgcc ggccgacgag
     5521 ctcttgtccg gactgtttgg ccccactcgc cggctctaca agcggctggc cgagttcagc
     5581 cacttccagc accgcgaatg gtacgaccgc ctcgcccggc agccgtatcc ctggctggtg
     5641 cgtgccagcg aatcgttcgc cgccgctg
//
LOCUS       DASRJB010000697         7437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1891651, whole genome shotgun sequence.
ACCESSION   DASRJB010000697 DASRJB010000000
VERSION     DASRJB010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891651"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFB80_18515"
     CDS             <1..837
                     /locus_tag="VFB80_18515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35833.1"
                     /translation="GSETDPERDPRVKFIGFPREECISVNRRKQPLNLAPYEAELNRQ
                     WDEHGSRICALVTEPYLGGGGSFHPQHEYLQLLERFCREHDILFILDEVQANFGRTGE
                     LFAFSTYGVEPDIVVLGKGLGNGVAVSAAVGRADVFANMHYGEGSDTWSANPLASAAV
                     LATLDEYESTDVLQRAKQLSQVVERGLLKLCELPAVAHVRGEGVVWGVECRAVGDLPA
                     DAVARACVEACYRGDEQGRAIHLLGPLAGKVIRVSPPLVMPCNEAEEYLGVMYRLIAK
                     VG"
     gene            complement(869..1672)
                     /locus_tag="VFB80_18520"
     CDS             complement(869..1672)
                     /locus_tag="VFB80_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35834.1"
                     /translation="MRTLTRALVLVVATVTAPLAARGQDFRVDTELFVGTEKTPALET
                     LTIFTDGLVYDFVLTEPREVVILDTQRGRFSLLDESRQVRASMATQDLLDFTLALESH
                     AATGKNALLAFAARPKFQTAEKTVDENGQSLLELRLSGKPIEYVVLGARSERPEAAKI
                     YRHFADWYARLNATRPGNLPAGARLAVNEELGRRELLPREVTRIITPANPLARKLEVK
                     TRHLVNWTLSGEDRRRIERAGDWLATYRPISYDEYRGANRQPPAQQAKR"
     gene            complement(1743..3389)
                     /locus_tag="VFB80_18525"
     CDS             complement(1743..3389)
                     /locus_tag="VFB80_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35835.1"
                     /translation="MYASCPPKFHPVELKLADHDPDGTRRWQLMTWAFVALGVAIRLN
                     RYLLKFPLWGDELMLAENFLDRGYSDLMQPLNNIQCAPLLFLWIELAAIKLIGFSEWS
                     LRLFPAVCSIGGVFLFRHLASRIVQGLPLLIAVAIFAVSHLPIRYGAEVKPYASDLTV
                     SLVLLVLAVEWLRRPQQSRWLWALALLAPIAIGLSFPAVFVAGGICLGIAWPAWRAGW
                     RARFALAACNLATLGSFLLFAWLSLQGQFRSSGDYMQRYWADGFPPVTQPLKLAAWLL
                     LTHTGELLAYPIGGERCGSILTAICCGVGVWILARKRQHTLLCMFAGIALLALIAAAL
                     RRYPYGDNSRLVQYLAPPICLLAGLGAAGLITALGSAKSQRRAALVLTMLLGLGGTAI
                     VGFDLVHPYKYRADYVHRGFAQWFWSNQPPGADVISLRPEDFQRFSTNWDRCAYRCMQ
                     RIYAPQPGDAPNGPAPSGRPFRCVLYRSTDAKADEQALAAWLDDMRARYELVEQRRFT
                     VNLSIPRRKVIFGDYAVYEFAPRGGTSVARTPAEAGRLQR"
     gene            3581..4102
                     /locus_tag="VFB80_18530"
     CDS             3581..4102
                     /locus_tag="VFB80_18530"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HZN35836.1"
                     /translation="MALLAQNWPLVLFAAIIAVLVVVSLVSLRDGPLPYERRGGLLSP
                     AEINLLRSLQLAVREDWLIFSQVRLADVLKVRAKTRKYQSWNSRLQGKQLQFVLCDTE
                     TLDVKLAIELDEPATSTDGARRDRFVNVALAAAGVPLLRVRMQEKYETAALRKDIEDA
                     LGILRKKKRAYAG"
     gene            complement(4105..4527)
                     /locus_tag="VFB80_18535"
     CDS             complement(4105..4527)
                     /locus_tag="VFB80_18535"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZN35837.1"
                     /translation="MNDWGFKLLYDGQCPLCRLEAAWLSRWNRRGRLILEDISSPQFD
                     PAQYGVTHEELMGVMHGVLPDGRLVRRVEAFRQAYAAVGLGWLLAPTAWPGLRWIFDG
                     LYSLFARLRVPLGRLLAGPCESGTCSVPARPPGGGRDV"
     gene            4632..5333
                     /locus_tag="VFB80_18540"
     CDS             4632..5333
                     /locus_tag="VFB80_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN35838.1"
                     /translation="MANRPGGALATRPLQFIWIVDCSGSMQGKKIEALNFAIREAIPA
                     MQDVARENPNAQVLMRAVRFSDSAHWHVAQPTDVHEFKWTDLTADGVTDMGQALGIVA
                     EALKMENMPPRGLPPVLVLISDGQPTDNFTKGLAKLMAEPWGVKAVRVAIAIGDDADL
                     NVLQQFIGNVEVKPLRADNARDLAKKIRWASTVPLKAASNPASRTQEQGDPAANVTLP
                     APPEPIGPVSADEVF"
     gene            5353..6231
                     /locus_tag="VFB80_18545"
     CDS             5353..6231
                     /locus_tag="VFB80_18545"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HZN35839.1"
                     /translation="MPTAVVQPWWALSASVCGASHARTGLPNQDAVKVVQDGGGLPAI
                     LAVSDGHGSAKCFRSHVGSQLAVDAAVAALDDFATGASEIPPEQIQASAALLPGAIVR
                     RWRAAVAEHHAANPIGESELAALEPAAAVSARQALDRGDPFLIYGATLVSALLGPTWL
                     LCLQLGDGEILTVSRGAAEVMQPIAVDESLIANETTSLCERDAEQNFRLHFRMLGDST
                     PALLLLATDGYPNSFETPAGFRQVASDLLGILDQDGAESVAQALPGWLEESSRLGSGD
                     DVSVAILYLPPAKEAP"
     gene            6228..>7437
                     /locus_tag="VFB80_18550"
     CDS             6228..>7437
                     /locus_tag="VFB80_18550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35840.1"
                     /translation="MNELLKPGQVIRTHYSGEPCQVERFLGGGGQGEVYRANWAGGQF
                     ALKWYFPQTATREQNVALQRLIQSHRPPSDRFLWPLDLALADGVRGFGYLMRLREPRF
                     KSLFDLVTQRIDPTFAALVTAAIQLADSFHKLHADGLCYRDISFGNAFFDPDTGEVLI
                     CDNDNVAENRSPVTGVLGTPDFMAPELVRMEALPSRQTDLFSLAVLLFYMFCTSHPLV
                     GKRILAIRSWDYPARKKMFGLEPLFIFDPDDDSNAAVDRSLDPYGEAGDNALATWPLY
                     PQFLRDLFTKAFTAGVRDADHGRVTEGEWKFTLGRLHDSLLASCSCGRQNFFDPAQLD
                     AAGRYRKACWNCGLIPPLPFRLHVGKLAIVLNPDAKLVAHHLEDHSDPGATAPVAEVS
                     RHPTNPNVLGL"
BASE COUNT         1279 a         2452 c         2515 g         1191 t
ORIGIN      
        1 ggcagcgaga ccgatccgga gcgcgacccg cgagttaaat tcatcggctt cccgcgcgag
       61 gagtgcatca gcgtcaaccg ccgcaagcag ccgctgaatc tcgcgcccta tgaggccgag
      121 ctgaaccgcc agtgggatga gcatggcagc cggatctgcg cgctagtcac cgagccctat
      181 ctgggcggcg gcggttcgtt ccatccgcag cacgagtatc tgcaattgct ggagcggttc
      241 tgccgcgagc acgacatcct gttcatcctc gacgaagtgc aggcgaactt cggccggacg
      301 ggcgagctgt tcgcgttcag cacctacggc gtcgagccgg acatcgtcgt gctgggtaag
      361 gggctcggga acggcgtggc ggtgagcgcc gctgtcggcc gggcggacgt attcgccaac
      421 atgcattacg gcgaaggctc ggacacctgg agcgccaacc cgctcgccag tgcggccgtg
      481 ctggccaccc tcgatgagta cgaatcgacc gatgtcctcc agcgagcgaa acagctctcg
      541 caggtggtcg agcgcgggct gctcaaactg tgcgaactgc cggcggtcgc tcacgtccgc
      601 ggcgaaggcg tcgtgtgggg cgtcgaatgc cgcgccgtcg gcgatctgcc ggccgatgcc
      661 gtggcccgcg cgtgcgtgga ggcctgctat cgtggcgacg agcaaggccg ggcgattcac
      721 ctgctgggac cgctggccgg caaggtcatt cgcgtcagcc cgccgctggt catgccgtgc
      781 aatgaggccg aggaatatct cggcgtcatg taccgattga tcgccaaggt cggctgatgc
      841 cgccgacgat ggcagcgtcg gctacggttt agcgtttggc ctgctgggcc ggcggctggc
      901 ggttagcgcc gcggtattcg tcgtagctga tcggccgata ggtcgcgagc cagtcgcccg
      961 cccgctcgat ccgccgccgg tcctcacccg acagcgtcca attcacgagg tgccgcgttt
     1021 tgacttccag cttgcgagcc agcgggttgg cgggcgtgat gatccgcgtc acctcgcgcg
     1081 gcagcaattc gcggcggccg agttcctcgt tgacggccag ccgggcaccg gcgggcagat
     1141 tgccgggccg cgtggcgttg aggcgggcgt accagtcggc gaagtggcga tagatcttcg
     1201 ccgcctcggg tcgctcgctg cgcgcgccga ggacgacgta ctcgatcggc ttgccggaca
     1261 gccgcagttc gagcagcgac tggccgtttt catcgaccgt tttttcggcg gtttgaaact
     1321 tgggccgggc ggcgaaggcc aagagagcgt ttttgcctgt cgcggcgtgc gattccaggg
     1381 cgagcgtgaa gtcgagcagg tcctgcgtgg ccatgctggc gcggacctgg cgcgactcgt
     1441 cgagcagcga gaagcgtccc cgctgcgtgt cgaggatcac gacctcccgc ggctcggtga
     1501 ggacgaaatc gtagaccagc ccgtcggtga agatcgtaag cgtctcgagc gcgggcgttt
     1561 tttccgtccc cacgaacaac tcggtatcga cgcgaaagtc ctgcccgcgg gcggccaggg
     1621 gcgcggtaac tgtagcgacg acgaggacca gcgcacgcgt gagcgttctc atgagcggtt
     1681 tctccagatt cagcggcgcg gagcgtagcg agcggcgcga tttgcgtcca gccgaatcgc
     1741 tgctagcgtt gcagcctacc ggcctcggcc ggcgtgcggg cgacgctcgt cccgccgcgc
     1801 ggcgcgaact catagacggc gtagtcgccg aagatgacct tccgccgcgg aatcgacaga
     1861 ttgaccgtga accggcgctg ttccaccagt tcgtacctgg cccgcatatc gtcgagccag
     1921 gcagccagag cctgctcgtc cgctttggcg tcggtcgagc gatacagcac gcagcggaag
     1981 ggccgcccgc tgggcgccgg cccatttggc gcgtcgccag gctgcggcgc gtagatccgc
     2041 tgcatgcagc gatacgcgca acgatcccaa ttggtggaaa agcgctggaa gtcctccggc
     2101 cgcaggctga tgacatccgc acccggtggc tggttcgacc agaaccactg ggcgaacccg
     2161 cgatgcacgt agtcggcgcg atatttgtac ggatgaacga ggtcgaatcc cacgatggcc
     2221 gtcccgccca gaccgagcag cattgtgagg accagcgcgg cgcggcgctg gctcttggcc
     2281 gaccccagcg cggtgatcag ccccgccgct ccgagcccag ccagcaggca gatcggcggt
     2341 gcgaggtact gcaccagccg cgaattgtcg ccatagggat agcgccgcag cgccgcggcg
     2401 atcagcgcca gcagggcgat ccccgcgaac atgcacagca gggtatgctg ccgcttccgc
     2461 gcgagaatcc agactccgac gccacagcag atcgccgtga gaatgctgcc gcagcgctcg
     2521 ccgccgatcg ggtaggcgag cagctcgccg gtgtgcgtga gcagcagcca tgccgccagc
     2581 ttcaggggct gcgtcactgg cggaaaaccg tccgcccagt aacgctgcat gtagtcgccc
     2641 gacgagcgga attgtccttg cagcgacagc caggcaaaca gcagaaagct gccgagggtc
     2701 gccaggttgc aggccgccag cgcgaagcgc gctcgccaac ccgcccgcca ggccggccag
     2761 gcaatcccca ggcagattcc tccggccacg aacacggcgg gaaacgacag gccgatcgcg
     2821 atcggcgcga gcagcgccag cgcccacagc cagcgcgact gctgcggccg gcgcagccac
     2881 tcgacggcca gcacgagcaa cacgaggctg acggtcaggt ccgaagcata cggcttcacc
     2941 tccgcgccgt agcgaatcgg caggtgcgag acggcgaaga tcgccaccgc gatgagcagc
     3001 ggcagcccct gcacaatgcg gctggccaga tgccggaaca ggaacacgcc gccgatgctg
     3061 cacacggccg gaaacagccg cagggaccac tcggagaatc cgatgagctt gattgccgcc
     3121 agctcgatcc acagaaagag cagcggagcg cactgaatgt tgttcagcgg ctgcatgagg
     3181 tcgctgtagc cgcggtcgag gaagttctcg gccagcatca gctcgtcgcc ccagagcggg
     3241 aacttgagca ggtatcgatt cagccggatc gcaacgccca aggccacgaa tgcccaagtc
     3301 attagctgcc agcggcgcgt gccatcaggg tcgtggtccg ccagtttcaa ctcgaccggg
     3361 tggaatttgg gagggcagct ggcgtacatc gctggctctc acggctgaat ggcaatgggg
     3421 gcgggagtat cgcgcaaagt gaaaagccag gcaatagagg aaaaccgcga tggctgtcct
     3481 gtggcaatct gtactagcgc ggccgggggc cgtggacgaa gtcatcggcg aactccgatg
     3541 cagataacgg gttgctgtcg ccgcgaggtt ttgatccgtc atggccctgc tcgcgcaaaa
     3601 ctggccgctg gtgctgttcg ccgcgatcat cgccgtgctg gtggtcgtgt cgctggtttc
     3661 gctgcgggat ggtccgctgc cctacgagcg tcgcggcggg ctgctctcgc cggcggagat
     3721 caacctgctg cgttcgttgc agctggcggt ccgcgaggat tggctgatct tctcgcaggt
     3781 ccgcctggcc gacgtgctca aggtccgcgc caagacgcgg aagtaccaga gctggaacag
     3841 ccggctgcag ggcaagcagc tccagtttgt gctgtgcgat accgagacac tggatgtcaa
     3901 gctggcgatc gagttggacg agcccgcgac cagcaccgat ggtgcgcggc gggaccgctt
     3961 tgtgaacgtc gcgctggcgg ccgcgggcgt gccgctgttg cgggtgcgta tgcaggagaa
     4021 atacgagacg gccgccctgc ggaaggacat cgaagacgcg ctgggaatcc tgcgcaagaa
     4081 gaagcgggcc tacgcaggtt gagcctagac gtctcgtccg ccgccgggcg gacgggccgg
     4141 cacgctgcac gtgccgctct cgcacggccc ggccagcagc cggcccagcg gcacgcgcaa
     4201 acgggcgaag agcgaataga gcccgtcgaa gatccaccgc agtcccggcc aggcggttgg
     4261 cgcgaggagc cagcccagcc cgaccgcggc gtacgcctgc cggaaggctt cgacgcggcg
     4321 gacaagacgc ccgtcgggca gcacgccgtg catcacgccc atcagctctt cgtgggtcac
     4381 gccgtactgc gccgggtcga actgcgggct ggagatgtcc tccaggatca gccgtccgcg
     4441 gcggttccag cgcgagagcc aggccgcctc caagcggcag agtgggcact ggccgtcgta
     4501 cagcagtttg aaaccccagt cgttcatggc agggcctttg cctcccaacc gcattcgccg
     4561 ccggctgata accgcgactt gaactgctat cttatcgggg acattctatt cctccgattt
     4621 cgaggtctgc gatggccaac cgacctggtg gcgcacttgc cacacgaccg ctccagttca
     4681 tctggatcgt ggactgctcc ggctcgatgc agggcaagaa gatcgaggcc ctgaacttcg
     4741 cgattcggga ggcgattccc gcgatgcagg acgtggcccg cgagaatccc aacgcccagg
     4801 tgctgatgcg ggcggtccgc ttctcggaca gcgcccactg gcacgtggcc cagccgacgg
     4861 acgtgcacga attcaagtgg accgacctga cggccgacgg cgtcaccgac atggggcagg
     4921 cgctgggcat cgtggccgaa gcgctgaaga tggagaacat gccgccgcgg ggcctgccac
     4981 cggtgctggt gctcatttcc gacgggcagc cgacggacaa tttcaccaaa ggcctggcga
     5041 agctgatggc cgagccgtgg ggcgtgaagg ctgtccgcgt ggcgatcgcc atcggcgacg
     5101 acgccgacct gaatgtgctc cagcagttca tcggcaacgt cgaggtcaag ccgctgcggg
     5161 ccgacaacgc ccgcgacctg gcgaagaaaa tccgctgggc ctcgaccgtt ccgctcaagg
     5221 ccgcgagcaa tccggccagc cgcacccaag agcagggcga ccccgcagcc aacgtgacgc
     5281 tgcccgcgcc gcccgagccg atcgggccgg tttcggccga cgaagtcttt tagttggcga
     5341 ggcagcccac gcatgccgac ggcagtcgtt cagccctggt gggctctgag tgcgagcgtc
     5401 tgcggcgcat cgcacgcgcg gacaggcctg cccaatcagg atgcggtgaa ggtcgtgcag
     5461 gacggcggcg gactgcctgc aatcctcgcg gtgtcggacg gccacggcag cgcgaagtgc
     5521 tttcgcagcc atgtcggctc gcagctggca gtggatgcgg cagttgccgc gctagatgac
     5581 ttcgcgacgg gcgccagcga aataccaccg gagcaaatcc aggccagcgc cgcgctgctg
     5641 cccggcgcga tcgtccgccg ctggcgagcg gccgtcgccg agcaccacgc ggcaaacccc
     5701 atcggcgaat cggaacttgc cgctctggag ccggccgcgg cggtgagcgc acggcaggcc
     5761 ctcgatcgcg gcgacccgtt tttgatctac ggcgccacgt tggtaagcgc gctgctggga
     5821 ccaacctggt tgctatgcct gcaattgggg gatggggaaa tcctgacggt atcgcgcggg
     5881 gctgccgaag taatgcagcc gatcgccgtg gacgagtcgc tgattgccaa cgaaaccacg
     5941 tcactgtgcg aaagggacgc cgagcagaac ttccggttgc atttcagaat gctgggcgac
     6001 tcgactcccg cgctcctgct gctggccacc gacggctatc cgaactcgtt cgagacgccg
     6061 gccggttttc gccaggtggc gtccgacctg ctgggcatcc tcgaccagga cggcgcggaa
     6121 tccgtcgccc aggcgcttcc cggctggctc gaagagtcct cacggctggg cagcggcgac
     6181 gacgtgtcgg tggcgatcct atatttgcct ccggcaaagg aggcgccatg aacgagctgc
     6241 tgaagcccgg ccaggttatc cgcacgcact actccggcga gccgtgccag gtcgagcggt
     6301 ttttgggcgg cggcggccag ggagaggtct atcgagcgaa ttgggccggc ggtcaatttg
     6361 ccctcaagtg gtactttccg caaaccgcga cccgcgagca gaacgtcgcc ctgcagcggc
     6421 tgattcagag ccaccgcccg cccagcgacc gcttcctgtg gccgctcgac ctggccctgg
     6481 ccgacggcgt ccgcggcttc ggctacctga tgcggctgcg cgagccgcgg ttcaagagcc
     6541 tgttcgacct ggtgacgcag cggatcgacc cgacgttcgc ggcgctggtg accgcggcga
     6601 tccagctggc cgacagcttt cacaagctgc acgccgacgg cctctgctac cgcgacatct
     6661 cgttcggtaa tgctttcttc gaccccgaca ccggcgaggt gctgatctgc gacaacgaca
     6721 acgtcgccga gaaccgctcg ccggtcaccg gcgtcctggg cacgccggac ttcatggcgc
     6781 cggagctggt ccgcatggaa gctctgccca gccggcagac cgacctcttc tcgctcgcgg
     6841 tgctgctgtt ctacatgttc tgcacaagcc atccgctggt cggcaagcgg atcctggcga
     6901 tccgcagctg ggactatcca gcccgcaaga agatgtttgg cctggagccg cttttcatct
     6961 tcgatcccga cgacgattcg aacgccgccg tcgatcgctc gctcgatccc tacggcgagg
     7021 cgggggacaa cgctctggcg acctggccgc tctacccgca gtttctccgc gacctgttca
     7081 ccaaggcctt cacggccggc gtgcgcgacg ccgatcatgg ccgcgtcacc gagggcgagt
     7141 ggaagttcac gctcggccgc ctgcacgaca gcctgctcgc gtcgtgttcg tgcggcaggc
     7201 agaatttctt cgacccagcg cagctcgatg cggccggccg ctaccgcaag gcctgctgga
     7261 attgcgggct catcccgccg ctgccgttcc ggttgcacgt cgggaagctg gcgattgtgc
     7321 tcaatcctga cgcgaagctc gtggctcatc atctcgaaga tcactccgac cccggcgcga
     7381 cggctcccgt ggccgaagtc agccgccatc cgacgaatcc caacgttctg ggcctca
//
LOCUS       DASRJB010000698        10095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3586428, whole genome shotgun sequence.
ACCESSION   DASRJB010000698 DASRJB010000000
VERSION     DASRJB010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586428"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFB80_18555"
     CDS             <1..286
                     /locus_tag="VFB80_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35841.1"
                     /translation="HPYYQLGPRPEYPVGLVCGHFEKPPPPENGRMNRHDRRKRRLQG
                     VHDHSASCYASAAAQDVTQWIAEHPAEHEGLKAVYPAIWRDVVRDLELAH"
     gene            333..1556
                     /locus_tag="VFB80_18560"
     CDS             333..1556
                     /locus_tag="VFB80_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35842.1"
                     /translation="MLKGVLGTAAGGVVMNWGGLTQTALGAEAAKQQKHCIYLFMNGG
                     ASQFETFDMKVGRPTGGVFRPISTNVAGTQICELMPQMAQQMDKIAVIRSMKTSEVDH
                     PGGIYLMHTGYRPSANVRFPEFGSIVAKYQGREATALPNFIKIFGHGDAGSGFLGPKY
                     QPFEISPEGQLPPFSGSSLSPEKELRRHELRNFLEEKYAEQNPSELARMHREAYEAAR
                     RLQGARQAFKIDSEWEKYKALYGDSAMGRRCLLARCLVEAGVPFIEIGQSSYDSHADN
                     FAWHKGLVPPMEHAWAGLMTDLADRGLFDKTLIVWAGEIGRTPNINNRAGRDHYVRCW
                     SAALAGCGIKGGQMYGESDADGVEVKDNPVSEGDFFATIYKALSIDPTTQNYAGVRPI
                     PLAPFGSKVVGDLLA"
     gene            1617..2900
                     /locus_tag="VFB80_18565"
     CDS             1617..2900
                     /locus_tag="VFB80_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35843.1"
                     /translation="MLRVNNSKLLWQLTFEGAWPTAVAFLGGSRKLAAANQEGQIYVW
                     DLPEEPPAFEPDKKNAERKAPNHWPARRLDGHTNGVTRLIAAADGQTLVSSSLDHSIR
                     LWQPSGAASGTADAILDGDERKSRAKRERKDEILAAPGVTVETLASAHVLGGPASGKR
                     ELPDKDPSRGEQNDREADASRSPGGHEDWVLSLGMSSDGRRLISGDASARVIVWDLTE
                     RREIVRFSGHAWNWIVAAALSADGQTALVSEYRYKRDDFDIPAAALRLFDAASGNEKL
                     DILKAMFPKLKPEENSYGSAQVWRKFIAHGLVAADFSPDGSLIALAQGGETDTGKVQL
                     VETATGKLVREVSGHQYGATDVKFSADGKLVLSTGRDTTLRICQVEDGKEVAALGTPR
                     GGQFKDWLAALAVSPDESTVAAADIAGLVHVWKLA"
     gene            complement(2981..4438)
                     /locus_tag="VFB80_18570"
     CDS             complement(2981..4438)
                     /locus_tag="VFB80_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35844.1"
                     /translation="MTEYAIIFQVLAALVALFFIFLTYMNTKTWKWVHVTMTFMVFAA
                     AIAFCFYAAAALKTRAAWIKLHDGLEKDVETTAQQLELLTRGDPRDVEGKTPSIASLR
                     EELSRTIIDRGRVWRGCPPQAFDPGRGTATVLTTPPPDPNLPPPPPKKNNIQVKTVLH
                     AFREALTTAGLSVPVAYIGEFEATAVTDNSVTMSATMPLSADQAAAAGAPPVTWALYE
                     TCPVDGHEWFTGMNNQAALETMIPGNKQDPAYQKMIQSYVRDGQRAEDTDPPENVWFE
                     VKFLRQHVVVVDAPAAGTSLDASSDLFPFNADGQAVPERLRRRNPAGPVTFGPGPGQI
                     QTAILDQQTAEPLVSAGIAEKIRPIYRRKLNDYEQRLHAIYLRTLDINGRVRQLTIDD
                     QTTVATTTKANQQSMLVAELKTKLTDDLTKAQFEQSELKKYSDAIEARLAAVQTQLSE
                     LYKSNKAISRELVKLNNDLTSEIERRTRESTAKSP"
     gene            4721..5239
                     /locus_tag="VFB80_18575"
     CDS             4721..5239
                     /locus_tag="VFB80_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN35845.1"
                     /translation="MDQHLLLPRRGADAAPHGEQSDQLLGLIHWLSAASRQLRRRLAD
                     VAASCELSDGELLVIWLCHGAGNVQVDLAAAIGVSPAQMSGLVERLGQRGLVAMNRSV
                     RDRRRQVWRTSAAGEALLGQVAAPLANLTAALGRQVDSAEQALAQSLCQRLASAAAQQ
                     AQDEQRCGKEAA"
     gene            5241..8171
                     /locus_tag="VFB80_18580"
     CDS             5241..8171
                     /locus_tag="VFB80_18580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN35846.1"
                     /translation="MPHHSHRERFRPAAAWLALLLALAGGLSTGCTRAFYRRQADIDA
                     YSLVREKANHPHWALNNYSISVDPRSRMYDPYAIDCPPIPPDDPTAHQLMHCVDNKRG
                     WPFWHDNGERPYIENPAWPEYIEVDTEGIVRVSGDDAVRLALLHSRNYQQALESLYLS
                     ALDVSFERFRFDAQFFAGYSLFSTFDGRARTGAARQTGTGGNSSSQFTASTFSTSNPA
                     GWQIRKLTTTGGTLIADFANSLVWQFSGPDDFRGSSLLDFTLIQPLLRNAGRDRVLER
                     LTRAERVLLSNVRQMEQYRQGFYVDIMTGSGGQQAPSRAGGQFGSGFEGFTGVATVGF
                     GGGGGGAAAAAAPGAQGGYLALLQSQRQISNQEDNVRRLRRNVSRLTTLLEEQAAAQT
                     GEYLSQGLNVAQARQALLSAETNLLTLQNTYQSDLDAFKVQDIGLPPMVCLQPVDNLL
                     DQFVLIDPTIIRLPEDWEAILTAHAPARNDVPERIQQNVEVVEPMGAAPFCRLKRYPE
                     LDQDLETLRPALADMRQFADRIVGEHFPVIQRDIETLRASVPRRKASLQRLIARIQEL
                     QRTKCELLPLTLNPLDSIDPDVSAAQTQIGRLEAALADAEKSLTTLAASFERYAASLA
                     ARQQVLDQLIQNKTQTPEELFQKLVKEVFNPLYECNKTRVITFDIVEDISRELTELQL
                     LQALARTESVELGEIDLGAEQALELARKYRRDWMNARASLVDSWRLIQFNADQLQSTL
                     DLFFSGDVLNITDNPFRLRGTTGRLRVGVQFDAPITRLAERNTYRQSLIEFQQARRNY
                     YAFEDNVARSLRSQLRSLLTFQMNFELQRLAVLQAARQVMLNAFIDQENQRALTTRAT
                     AARDAVQALSDLLNAQNQFMQVFVSYEVLRMTLDLQLGTMQLDEEGLWIDPGRIGPEY
                     GQIDPWLRQFNQQHEPVRGGTGGGRSGQPEELAPPPASAGPPAGIPITPPLPLLGPDS
                     LPAP"
     gene            8272..8760
                     /locus_tag="VFB80_18585"
     CDS             8272..8760
                     /locus_tag="VFB80_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35847.1"
                     /translation="MKSARSLIGVVTLVACSLAAAGVAHAQGDAEVEAKFIAMLKNAT
                     LKGTWAPVAKGVLGNERKDDSYQIARVEKGEGGKWTIVSLFVVRERQLEFPIACSVKF
                     AGDTAVLILDNVRAGPGNANWSARILFFDDVYTGRWWETANKEHGGTIAGTITRAEAK
                     SP"
     gene            8788..9669
                     /locus_tag="VFB80_18590"
     CDS             8788..9669
                     /locus_tag="VFB80_18590"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZN35848.1"
                     /translation="MNALIRVAAVVAILGLCRAGFAGEPAKDSAGQVTRIGAVAYSPA
                     AVTIFQGLTRYLNKHGLPADYVLYSNYDSLVAALAKGEIDIAWNTPLAHAKFHVQSQC
                     SSQTLVMRDVDVGIRSVLVARSDAKITSPGDLAGKKLVLGSSQAAEATVLPLHFLKKE
                     GADLSQVILVSLDAEVDSQGNPCASPQHVLQALRSGRGDAGIITADLWERVRSETSAP
                     VSLRAIWTSPPFSHCVFTAAAKFDKDRALAFTKLMRAMDPNDSATRDVMRLEGTKQWL
                     PGSDTGFKDLVEALGGK"
     gene            9899..>10095
                     /locus_tag="VFB80_18595"
     CDS             9899..>10095
                     /locus_tag="VFB80_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35849.1"
                     /translation="MNAPLVILLLALQGLGQAPPAADNPDTKAEAVEARAVAKLLAAE
                     YVVEQGKSSGVKLQQTPEPVLR"
BASE COUNT         1773 a         3345 c         3317 g         1660 t
ORIGIN      
        1 ccatccctat taccaactgg gaccacgacc ggaatatcct gtcggattgg tgtgcggcca
       61 ctttgagaaa ccgccgccgc cagaaaacgg gcgcatgaac cgccacgatc gccggaaacg
      121 ccgtttgcaa ggagtccatg atcacagcgc cagttgctat gcgtcggccg cagcacaaga
      181 tgtgacacag tggattgcag agcatcccgc ggagcatgaa ggcttgaaag cggtttatcc
      241 ggcaatttgg cgtgatgttg tccgagattt ggagctagcg cactaacatg catccaattt
      301 gcaatcgcac cgaacacgtt tcccggcgcg ccttgctcaa gggcgtcctc ggcacggccg
      361 cgggcggcgt ggtgatgaac tggggcgggc tgacacagac ggcgctggga gcggaggcgg
      421 cgaagcagca gaagcactgc atctacctgt tcatgaacgg cggggcgagc cagttcgaga
      481 cctttgacat gaaggtcggg cggccgacgg gcggggtgtt ccggccgatc tcgacgaacg
      541 tcgccggcac gcagatctgc gagctgatgc cgcaaatggc gcagcagatg gacaagatcg
      601 cggtgatccg ctcgatgaag accagcgagg tcgatcatcc cggcggcatc tacctgatgc
      661 acaccggcta ccgcccgagc gccaacgtgc ggttcccgga gttcggctcg atcgtcgcca
      721 agtaccaggg ccgcgaagcg accgccctgc ccaacttcat caagattttc ggtcacggcg
      781 acgccggcag cggcttcctc ggcccgaagt atcagccgtt cgagatcagc cccgaggggc
      841 agctgccgcc gttttctggc tcgagcctct cgccggagaa agagctgcgg cggcacgagc
      901 tgcggaattt tctcgaagag aaatacgccg agcagaatcc ttcggagctg gcccgcatgc
      961 accgcgaggc gtacgaagcg gcccgccggc tgcaaggcgc gcggcaggcc ttcaaaatag
     1021 atagcgagtg ggagaagtac aaagcgttgt acggcgacag cgcgatgggc cgccgctgcc
     1081 tgctggcccg ctgcctggtc gaggctggcg tgccgttcat cgagatcggc cagagcagtt
     1141 acgactcgca cgccgacaat ttcgcctggc acaagggcct cgtgccgccg atggagcacg
     1201 cctgggccgg cctgatgacc gatctggccg accgcggcct cttcgacaag acgctgatcg
     1261 tgtgggccgg cgagatcggc cgcacgccga acatcaacaa ccgcgccggt cgcgaccact
     1321 acgtccgttg ctggtccgcc gcgctcgccg gctgcggcat caagggcggc cagatgtacg
     1381 gcgagtcgga tgccgacggc gtcgaggtca aagacaaccc ggtcagcgaa ggcgacttct
     1441 tcgcgacgat ttacaaggct ctgtcgatcg acccgacaac gcagaactac gccggcgtgc
     1501 ggccgattcc gctggcgccg tttgggtcga aggtggtggg cgacttgctg gcgtagagca
     1561 agcgtcctcg cttgctcacc gtcgcccctg aaacacgctg cacaagcgag acgcttatgc
     1621 tacgagtcaa caactccaag ctcctctggc agctcacgtt cgaaggcgca tggccgacgg
     1681 cggtcgcgtt cctgggcggc agccgcaagc tggcggcggc gaatcaggag gggcagattt
     1741 acgtctggga tttgcccgag gaaccgccgg ccttcgagcc ggacaagaag aacgccgagc
     1801 gcaaagcacc gaaccactgg ccggcgcggc ggctggacgg gcacaccaac ggcgtgacgc
     1861 ggctgatcgc ggcggccgac ggccagacgc tcgtttcctc cagcctcgac cattcgatcc
     1921 gcctctggca gccttcaggc gccgccagcg gaacagccga cgccatcctc gacggcgacg
     1981 agcggaagag ccgggccaag cgggagcgga aggacgagat cctcgccgcg ccgggcgtga
     2041 cggttgagac gctggcgtcc gcgcatgtgc tgggcggacc ggcgagcggg aagcgtgagc
     2101 ttcccgataa agatccctcg cggggcgagc aaaatgatcg ggaggctgac gcctcccgct
     2161 cgccgggagg acacgaggat tgggtgttgt cgctcggcat gagcagcgac ggccggcggc
     2221 tgatcagcgg cgacgcgtcg gcgcgggtca tcgtgtggga cctgacggaa cggcgcgaga
     2281 tcgtgcggtt cagtggccac gcctggaact ggatcgtcgc cgcggcgctt tcggccgacg
     2341 gacagaccgc gctggtcagc gagtaccgtt acaagcgcga cgatttcgac attccggccg
     2401 ccgccctgcg gctcttcgac gcggccagcg gcaacgagaa gctcgacatc ctgaaggcga
     2461 tgttccccaa gctcaagccg gaggagaatt cctacggcag cgcccaggtg tggcggaaat
     2521 tcatcgccca cggcctggtc gccgcggact tctcgcccga cgggtcgctc atcgcgctgg
     2581 cccaaggcgg cgaaaccgac acgggcaagg tgcagcttgt cgagacagcc acgggcaagc
     2641 tcgtccgcga agtctcgggc catcagtacg gcgcgaccga cgtaaaattc tcggccgacg
     2701 gcaagctggt cctctcgacg ggccgcgaca cgaccctccg catctgccag gtggaggacg
     2761 gcaaggaagt cgccgccctc ggtacgccgc gcggcggtca gttcaaggac tggctcgcgg
     2821 cgctggccgt ctcgccggat gagagcacgg tcgccgcagc cgacatcgcg ggcctcgtgc
     2881 acgtctggaa gctggcttga cccaaagctg cgaccattgg tcgcggctca cttgcaacga
     2941 gctgggcaga agaccgcgac cactggtcgc ggctttgtta ctacgggctc ttggccgtcg
     3001 attcgcgcgt ccgccgctcg atctcgctgg tcagatcgtt gttgagcttg accagctcgc
     3061 ggctgatggc cttgttcgac ttgtagagct cgctgagctg ggtctgcacg gcggccagcc
     3121 gggcctcgat cgcgtccgaa tacttcttca gctcggactg ctcgaactgg gccttggtca
     3181 gatcatcggt cagcttggtc ttcagctcgg cgaccagcat cgattgctgg ttggccttgg
     3241 tcgtggtggc gacggtcgtc tggtcgtcga tcgtcagctg ccggacgcgg ccgttgatgt
     3301 cgagcgtgcg gaggtaaatc gcgtgcagcc gctgttcgta gtcgttcagc ttgcggcgat
     3361 agatcgggcg gatcttctcg gcgatgccgg ccgagacgag cggttcggcc gtctgctggt
     3421 cgagaatcgc ggtttgaatt tgccccggcc cggggccgaa cgtgaccggc cccgcgggat
     3481 tgcggcggcg cagccgctcg ggcacggcct ggccgtcggc gttgaacggg aacaaatcgg
     3541 agctggcgtc gagcgacgtg cccgcggccg gagcgtccac aacgacaacg tgttggcgaa
     3601 ggaacttgac ttcgaaccag acgttctccg gcggatcggt gtcttccgcc cgctggccgt
     3661 cgcggacgta cgactgaatc atcttctggt aggccgggtc ctgcttgttg ccgggaatca
     3721 tcgtctccaa ggcggcctgg ttgttcatcc ccgtaaacca ctcgtggcca tcgacggggc
     3781 acgtttcata cagcgcccag gtgaccggcg gcgccccggc tgcggcggcc tgatcggcgg
     3841 acagcggcat cgtcgccgac atcgtcaccg aattgtccgt cacggcggtg gcctcgaact
     3901 cgccgatgta ggccaccggg accgaaaggc ccgcggtcgt gagggcctcg cgaaacgcgt
     3961 gcagcacggt cttgacctgg atgttgttct tcttcggcgg cggcggaggc agattcggat
     4021 cgggaggcgg cgtcgtgagc acggtcgccg tgcccctccc cggatcgaaa gcctgcggcg
     4081 ggcagccccg ccacacgcgg ccgcgatcga tgatcgtccg gctcagctcc tcgcgcagcg
     4141 acgcgatcga cggcgtcttg ccttccacgt ccctgggatc gccgcgcgtc agcagctcca
     4201 gctgttgcgc cgtggtttcc acatccttct cgagaccgtc gtgcagcttg atccacgccg
     4261 cccgcgtctt cagggccgcc gcggcgtaaa agcaaaacgc gatcgccgcc gcgaacacca
     4321 tgaacgtcat cgtgacgtgg acccacttcc acgtcttcgt gttcatgtac gtcaagaaga
     4381 tgaagaacaa cgccaccagc gcggcaagca cctgaaagat gatggcgtat tcagtcatcg
     4441 cgcactgccg ctgctgatag gtcgggacgg acggacgccg tcagccggaa tctcgatgat
     4501 aaatagggct gtttggggtg tcaagtttga gcgggcaggc tgcgcaaagc gcaagtcttg
     4561 gctggggacg gacttccatt cgccgccccg ccaaccgcgc cggcgaccct tctttccttg
     4621 caatcgttca agttggaacg gtttattccc gaatcattcc ttagtgaagc gaccgcgaga
     4681 gtttgccggt tgcgcagact ttccggcccg agaggcacgg atggatcaac acctcctact
     4741 tcctcgccgc ggggccgatg ccgcgccgca cggcgagcag tccgatcagc tcttggggct
     4801 gatccactgg ctcagcgccg ccagccggca gttgcgccgc aggctggcag acgtcgcggc
     4861 gagctgcgag ctgagcgatg gcgagctctt ggtgatctgg ctctgccacg gcgcggggaa
     4921 cgtgcaagtc gatctggccg cggcgatcgg cgtttctccg gcccagatga gcggtctggt
     4981 cgagcggctg gggcagcgcg gcctggtggc gatgaaccgc tcggtccgcg accgccgccg
     5041 ccaggtctgg cggacttcag cggccggcga ggcgctcctg ggccaggtcg ccgcgccgct
     5101 ggcaaacctg acggcggcgc tcggccgcca ggtcgattcc gccgagcaag cactggccca
     5161 gtcgctctgc cagcggctgg cgagcgccgc ggcgcagcag gctcaggatg agcagcgctg
     5221 cggcaaggag gcggcctgac atgcctcacc actcgcatcg cgaacgcttc cgcccggcgg
     5281 ccgcctggct agcgctgctg ctggcgcttg cgggcggcct ttcgaccggc tgcacgcggg
     5341 ccttctatcg ccgacaggcc gacatcgacg cttactcgct ggtccgcgag aaggcgaatc
     5401 atccgcactg ggccctgaac aactactcga tctcggtcga tccgcggtcg cggatgtacg
     5461 acccctacgc gatcgactgc ccgccgattc cgcccgacga tccgaccgcg caccagctga
     5521 tgcactgcgt cgataacaag cgcggctggc cgttctggca cgacaacggc gagcggccct
     5581 acatcgaaaa ccccgcctgg ccggagtaca tcgaagtcga taccgaaggc atcgtgcggg
     5641 tcagcggcga cgacgccgtg cggctggccc tgctgcactc gcgcaattac cagcaggccc
     5701 tggagtcgct ctacctttcg gcgctcgacg tgagcttcga gcggttccgc ttcgatgcgc
     5761 agttcttcgc cggctattcg ttgttcagca ccttcgacgg ccgggctcgc acgggcgcgg
     5821 cccggcagac cgggacgggc ggaaactcga gcagccaatt cacggcgagc accttttcga
     5881 cctccaatcc tgccggctgg cagatccgca agctgaccac cacgggcggc acgctcatcg
     5941 cggattttgc caactcgctc gtctggcagt tttccgggcc cgacgatttc cgcggcagct
     6001 cgctgctcga cttcacgctg attcagccgc tgctgcggaa cgccggccgc gaccgcgtgc
     6061 tggagcgtct cacccgcgcg gagcgcgtgc tgctctccaa tgtgcggcag atggagcaat
     6121 accgccaggg tttctatgtg gacatcatga ccggcagcgg cggacagcag gccccgtcgc
     6181 gggccggcgg ccagttcggc tctggttttg aaggattcac gggcgtggcc acggtcggct
     6241 tcggcggcgg cggcggcggc gctgccgcgg cggctgcacc gggcgcccag ggcggttacc
     6301 tggcgctgct ccagagccag cgccaaatca gcaaccagga agacaacgtc cgccgcctgc
     6361 ggcgcaatgt ttcgcggctg accacgctgc tggaagagca ggcggcggcg cagacgggcg
     6421 agtatctcag ccagggcctg aacgtagccc aggcccgcca ggctctgctc agcgccgaaa
     6481 ccaacctgct gacgctccag aacacctatc aatccgacct cgatgcgttc aaggtgcagg
     6541 acatcggtct gccgccgatg gtctgcctgc agccggtcga taacctgctc gaccagttcg
     6601 tgctcatcga cccgacgatc atcaggcttc cggaggactg ggaggcgatc ctgaccgcgc
     6661 acgcgccggc gcgtaacgat gtgcccgagc ggattcagca gaacgtcgaa gtcgtcgagc
     6721 ccatgggcgc cgcgccgttt tgccgcctga agcggtatcc cgagctggac caggacctgg
     6781 aaacgctgcg tccggcattg gccgacatgc ggcagtttgc cgaccggatc gtcggcgagc
     6841 actttccggt catccagcgc gacatcgaga cgctccgcgc ctccgtgccg cggcggaagg
     6901 cgtccctgca gaggctgatc gcccgcatcc aggaactcca gcggaccaag tgcgagctct
     6961 tgcccctgac cctcaacccg ctggattcga tcgacccgga cgtcagtgcc gcccagaccc
     7021 agatcggccg cctggaagcc gccctggccg acgcggagaa atcgctgacc acgctggccg
     7081 ccagcttcga gcggtacgcc gcttcgctgg ccgcccgcca gcaggttctc gaccagctga
     7141 tccagaacaa gactcagact cccgaggagc tgttccaaaa gctcgtgaag gaggtcttta
     7201 atccgctcta cgagtgcaac aagacgcggg tcatcacctt cgacatcgtg gaggacatca
     7261 gccgggagct gaccgagctg cagctgctgc aggcgctggc ccgcaccgag agcgtcgagc
     7321 tgggcgagat cgacctgggg gccgagcagg ccctggagct ggcccgcaag taccgccgcg
     7381 actggatgaa cgcccgggcc agcctcgtcg attcctggcg gctcattcag ttcaacgccg
     7441 accagctgca gagcacgctc gacctgttct tcagcggcga cgtcctcaac atcaccgaca
     7501 atccgttccg cctccgcggc accaccggcc gcctgcgggt cggcgtgcag ttcgacgccc
     7561 ccatcacgcg gctggccgag cggaacacct accggcaatc gctgatcgaa ttccagcagg
     7621 cccgccggaa ctattacgcg ttcgaggaca acgtggcccg cagcctgcgg tcccagctcc
     7681 gcagcctcct cacgttccag atgaattttg agctccagcg gctggccgtg ctccaggccg
     7741 cccggcaagt catgctgaat gcctttatcg accaggagaa ccagcgggcg ctcaccactc
     7801 gcgccacggc cgcccgcgac gcggtccagg ccctcagcga cctgctcaac gcccagaacc
     7861 agttcatgca ggtgttcgtg tcgtacgaag tgttgcggat gacgctcgac ctccagctgg
     7921 gcacgatgca gctcgacgag gaaggcctgt ggatcgatcc aggccgcatc gggcccgagt
     7981 acggccagat cgatccctgg ctccgccagt tcaatcaaca gcacgaaccg gtccgcggcg
     8041 gaacgggcgg cggtagatcg ggccaacccg aggaactcgc tcctccgccc gccagcgccg
     8101 gtccgcctgc aggcattccc atcacgccgc cgctgccgct gcttgggccc gattcgctgc
     8161 cggcgccgta ggcgaattgg ggcgcagcga gaatcgcgcg caggctggtg atgcccgcgc
     8221 gggcggcgta gaatctggtc agatttcctg ctctgacact cgagggctgc catgaaatcc
     8281 gcgcgctctt tgatcggcgt ggtcacgctg gttgcctgtt cgctggccgc ggcaggggtg
     8341 gcccacgctc agggcgatgc cgaggtcgaa gcaaaattca tcgcgatgct gaagaacgcg
     8401 acgctcaagg gcacttgggc gccggtggcc aagggtgtgc tcggaaacga gcgcaaggac
     8461 gacagctacc agatcgcccg tgtcgagaag ggcgaagggg gcaagtggac catcgtctcg
     8521 ctgtttgtcg tgcgcgagcg gcaactggaa ttcccgattg cctgctccgt gaaattcgcc
     8581 ggcgacacag ccgtgctcat tctcgacaac gtccgcgccg gcccgggaaa cgccaactgg
     8641 tcggcccgca tcctgttttt cgacgacgtc tacaccggcc gctggtggga aacggccaac
     8701 aaggagcacg gcggcacgat cgccggcacg atcacgcggg ccgaagcgaa atcgccctga
     8761 acttctgcga ttgggaggtt gctcacaatg aacgcgctaa tcagagtcgc cgcagtcgtc
     8821 gccattttgg gcctctgccg ggcgggattc gccggcgaac cggcaaaaga ttccgcgggc
     8881 caggtgacgc ggattggagc cgtggcttat tcgccggccg cggtcaccat cttccagggg
     8941 ctcacccgct acttgaacaa gcacggcctg ccggccgact acgtgctcta ttcgaactac
     9001 gattcgctgg tggccgccct ggcgaagggc gagatcgaca tcgcctggaa tacgccgctg
     9061 gctcacgcca aatttcacgt gcagagtcag tgctcgagcc agaccctggt gatgcgcgac
     9121 gtggatgtcg gcatccgctc ggtgctggtg gctcgcagcg atgcgaagat tacgtcaccg
     9181 ggcgatctgg cgggcaaaaa actcgtgctc ggcagctcgc aggcggccga agccaccgtg
     9241 ctcccgctgc atttcctgaa gaaagaggga gcggacttga gccaggtcat cctcgtgagt
     9301 ctcgacgcgg aggtggacag tcaaggcaac ccctgcgcca gcccccagca cgtgctgcag
     9361 gccctgcgga gcggccgcgg cgacgccggg atcattacgg cggacctgtg ggagcgcgtc
     9421 aggagcgaaa cctccgcgcc ggtttcgctg cgggccatct ggacctcgcc ccccttcagc
     9481 cactgcgtct tcaccgccgc ggccaagttc gacaaagacc gcgccctcgc atttacgaaa
     9541 ttaatgcggg ccatggaccc gaacgacagc gccacccgcg acgtcatgcg gctggagggc
     9601 acaaaacagt ggctacccgg cagcgacaca ggcttcaagg acctggtcga ggcgctcggc
     9661 ggcaaatagc cgcggctggt gtcatctctt ccgcggcccg aacccggcgc gttgtggccg
     9721 gtctcccgac cgtgccacca aagttccgta gggtgggctg tgcccaccga ttcaagaatc
     9781 gcgggcgcag cccacctccg tcactttcgc tacgcttaag acgaatcgtc ttgaattccg
     9841 cggcgattgg gcgaaaatgc ggctagtccg cttccaggaa actaccgagg agtctgcgat
     9901 gaatgccccg ctagtgatac tgctcctggc cctgcagggt ctgggacaag caccccctgc
     9961 cgccgacaac cccgatacca aggccgaagc ggtggaggcc cgggccgtcg ccaagctgct
    10021 ggcggctgaa tacgtcgtcg agcagggcaa gtcatccggc gtgaagctcc agcagacgcc
    10081 cgagccggtg ctccg
//
LOCUS       DASRJB010000699         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3027118, whole genome shotgun sequence.
ACCESSION   DASRJB010000699 DASRJB010000000
VERSION     DASRJB010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027118"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFB80_18600"
     CDS             complement(<1..395)
                     /locus_tag="VFB80_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN35850.1"
                     /translation="MPAAGPEALAEDSSKTTSILRTTCEALDPGALLADLAGRDPDGS
                     SRFEIRRVLGAGSFGKVTLAFDRVLHREVAIKQPRERLAPRARDAFVHEARLAAQIRH
                     PGIVVVHDVAVDKSEIPYVVYEYVEGGSLR"
     gene            complement(1108..>2623)
                     /locus_tag="VFB80_18605"
     CDS             complement(1108..>2623)
                     /locus_tag="VFB80_18605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN35851.1"
                     /translation="ARLLAARVAGELKLLDKNGDGKLDEVEALARFGWSWNQYNQPAG
                     GESAANARQRARAVFAQLDKNKSGKLEFAEAGNLLRDQFERIDAEDKSADGAQLAAAR
                     SGAHFTSLDKDGDGRISRPEARGTALDRKFGSYDARDPATNKGDELLSSEELTQGLLK
                     SEPGRDGEIAAAELQDFYLKSNLQGDGELTANELAGTIGGYRNGMGDGPRGTPTVDGD
                     RLYAEGGNGDVTCLEAATGKTIWHVNLKAGFGGGTPGWGYSESPLVVGDLLIVTPGGK
                     QGTLLALNKHTGERVWQSGEETQGAHYSSPVLAEINGVKQIVQFARESVFGVALDSGK
                     PLWRYSAPANGTANCCSPIVEDNHVFASSAYGTGGGLAKIIPAGSTMQAEEVYFEKKM
                     ACHHGGMVKVGDHLYSNAAGALICMEFKTGKIAWQARSSGKGSLCVADGMLYLLGEGH
                     DLTLAEATPEAYRERGRLKLASHGKPAWAHPVVCGGRLYVRDQESLTAYDVRAQ"
BASE COUNT          405 a          875 c          882 g          461 t
ORIGIN      
        1 cggagcgagc ccccctcgac gtactcgtac acgacgtagg gaatctcgct cttgtccact
       61 gccacgtcgt gcaccacgac gatgccggga tgccggatct gcgccgccag gcgggcttcg
      121 tggacgaagg cgtcccgggc ccgcggcgcg agccgctctc gcggctgttt gatggcaacc
      181 tcgcgatgca gcacgcggtc gaacgccagc gtcaccttgc cgaagctgcc cgcacccagc
      241 acgcggcgaa tctcgaaccg gctgcttccg tccggatcgc ggcccgccag gtccgcgagc
      301 agcgcgccgg ggtccaaagc ttcgcacgtc gtccgcagga tggacgtcgt cttgctgctg
      361 tcctcggcca aggcctcagg cccggcagcc ggcacaggat cgcaagtggg atcaggctga
      421 ggcatagtcc gccggcggtg cgcactgcga tccatcgccc gggcgccggc atgggccaga
      481 gtatcctcgt ggcaagcccc aatcaactat cgccgcggaa agccgcacac cgacatccct
      541 gccgcaccac ctcggcaatt tttcgcctgc ccggcaccct tggggatctg gcacccggcg
      601 ttacggcggg tcggagacgc gccccgaaca gcgggccctg acagcgctgc aaccaccagc
      661 aaagcttgac cgtcagtgaa cataaggata caatcactcc aattggccga ccccgtcggc
      721 tgtcgctcct tggcaagctg gttgtgggaa agtagcggtg gcttcgccgt gcgggatcga
      781 gtgcgcctgg ctcgtgctta atttgcatat tctgcatata tgcagggctc aggaaatgcc
      841 gcaagtggct gctgggaagc aatttgcgtc aggaaatgcg gcctggggaa aatgccgcgt
      901 atgcatatgc aaggcacgca acctccggcc gcggcgggcg cgcataatgc gcataatggc
      961 tggcgggcag ttttcggcgc aagtcgctaa cggatcagga cttgtgacga gaaacggctg
     1021 ccggagaaat ttgcggagta tgcgcgcatg gcagccggcg tggatgccgg ggctaaagcg
     1081 ggcaggccct agccctagga ctagccgcta ctgggcccgg acgtcgtagg cggtcagcga
     1141 ttcctggtcg cggacgtaga gccggccgcc gcagacgacg gggtgggccc aggcgggctt
     1201 gccgtggctg gcgagcttga ggcggccgcg ctcgcgatag gcctcgggcg tggcctcggc
     1261 tagcgtgagg tcgtgcccct cgccgagcag gtagagcatg ccgtcggcga cgcatagcga
     1321 ccctttgccg gagctgcggg cctgccaggc gatcttgcca gtcttgaact ccatgcaaat
     1381 gagggcgcca gcggcgttgg aatagagatg gtcgccgacc ttcaccatcc cgccgtgatg
     1441 gcaggccatc ttcttctcga aatagacctc ctccgcctgc atggtgcttc cggcgggaat
     1501 gatcttggcc aggccgccgc cggtgccata agcgctcgag gcgaagacgt ggttgtcttc
     1561 cacgatcggc gagcagcagt tggcggtgcc gttagccggg gcggaatagc gccacagcgg
     1621 cttgccgctg tcgagcgcga cgccgaagac gctctcgcgg gcgaactgca cgatctgctt
     1681 gacgccgttg atctcggcca ggaccggcga cgagtagtgc gcgccctgtg tttcctcgcc
     1741 gctttgccag acccgctcgc ccgtgtgctt gttcagggcc agcagcgtac cctgcttgcc
     1801 gcccggcgtg acaatcagca ggtcgccgac gacgagcggc gactccgaat agccccagcc
     1861 cggcgtgccg ccgccgaagc cggccttgag attgacgtgc cagatggtct tgccggtcgc
     1921 cgcctccagg caggtgacgt cgccgttgcc gccttccgca tagagccggt cgccatcgac
     1981 cgtgggcgtg ccgcggggtc cgtcgcccat gccgttgcga tagccgccga tcgtgccggc
     2041 cagctcgttg gcggtcagtt caccgtcgcc ctggagattg ctcttgagat agaagtcctg
     2101 caattccgcg gcggcgattt cgccgtcgcg gccgggctcc gacttgagca ggccctgcgt
     2161 cagttcttcg ctggagagca gctcgtcccc cttgttggtc gccgggtcgc gggcatcgta
     2221 gctgccgaac ttgcggtcca gggccgtgcc gcgggcttcc ggccgcgaaa tccggccgtc
     2281 gccgtcctta tcgagcgagg tgaagtgagc gccgctgcgg gcggcggcca gttgcgcgcc
     2341 gtcggccgac ttgtcctcgg cgtcgatccg ctcgaactgg tcgcggagca ggttgccggc
     2401 ctcggcgaat tccagcttgc cgctcttgtt cttgtcgagc tgggcaaaca ccgcccgcgc
     2461 ccgctgccgg gcattggcag ccgactcgcc gccggccggc tggttgtatt gattccagct
     2521 ccagccaaag cgggccagag cctcgacctc gtccaacttg ccgtcgccgt ttttgtcgag
     2581 cagtttcagc tcgccggcga ctcgcgcggc cagtagccgc gcg
//
LOCUS       DASRJB010000700         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4152726, whole genome shotgun sequence.
ACCESSION   DASRJB010000700 DASRJB010000000
VERSION     DASRJB010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152726"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFB80_18610"
     CDS             <1..1250
                     /locus_tag="VFB80_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35852.1"
                     /translation="GSANGTNRASPGGPNMNALERLLQNRGSSGGNNPAGRIIDGAAA
                     GRPRGDTLANSLRLGNRTSAGDLGQFLGLNRRPAGSDLGNLRGNLARNGVAGDLAGSD
                     SVGGLRGRGLGRLNLNDSQVNLIKSNVNTAFRNELNVSNRINNFNNLNINNFQRFNNN
                     YFLNNPARAHYWNNWCGGIANHCNFNRFYGCFTPRFWATNFCYFPWRSSYYWWGARPW
                     SYWYGCPTWSTFCSFFPSYGWSQPYYYDYGVGGNVVYSDGNVLVNGSQVATAADYAAS
                     AAELATQPVPVNPDQPTDWLPLGTFALSTSETDKDPSRVVQLAVDKDGNINGTMVNRT
                     TNQTYPVQGRVDKETQRVAFTIGGSQEVVLETGLYNLTQQQTPLLVHGGGREETYLLY
                     RLEAPKTESTAPPAPAANPPLLP"
     gene            1401..2780
                     /locus_tag="VFB80_18615"
     CDS             1401..2780
                     /locus_tag="VFB80_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN35853.1"
                     /translation="MNRCPPFLLLALWPAWMLAADPWAASEPDPCLLVGTLSCASASC
                     HGRPEPASRPLLPEGAFGERSDQEHRRDSTSPRPPGPARQTGPTSGAATINQQEYLHF
                     LAGDPHAEAARRMTEPRYREVLRRASQYDPAAATRCAPCHDPLGHASESSHQSLGRGI
                     GCESCHGAARDWLAVHYEQDVSRERLVKLGLIDTRQVLARAGVCASCHVGSPGRDVNH
                     DLLAAGHPPLLFELASHQALIERKHWDDTARREREPNYEVQLWAAGQIASADAALSLL
                     AARAEQASKSGPWPEFAESDCRSCHRPLRAESGSSSGLPAWQSWNTAGISPLGSLDVL
                     RHEMQRSLVPQAAQVAGLAAAARTDLRQSVRLSPEGVLLCANGRPLDAAAVLERLDSA
                     QPPNFDRACQELAALLAAGRSLGAPSDLARLRRIAAELRRDAPITEIAARLAAARREI
                     SDNLPARQP"
     gene            2777..4432
                     /locus_tag="VFB80_18620"
     CDS             2777..4432
                     /locus_tag="VFB80_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN35854.1"
                     /translation="MTLCRALVAVCCACLALAQARAQYLTTESGCLSCHKVPPPDNLF
                     CRAVPVEVWAQTDKHSRAFHLLHETDPADPAKGDAKRTLVKQILGFDLAGAFDGPNYQ
                     RLKAGGDAETTRKVAVVKSCLRCHATWPIAADERYPTRPPAPLALGVSCQACHGPSAR
                     WDLPHREQVWRTVTPAGKAELGFTDVRTTAAKAGLCTSCHIGNLAEGKFVKHEWYAAG
                     HPPLPSFELATFEAEMPMHWIPLAKKPKFEFRDDRPADEGALADQLDTLKRAGVPAAA
                     IKPTYRAANFPDAAAKGLDPTRDLPQARSAVVAGAATLETYARLVGEYSRKSAAGEPG
                     FAWPELALYDCAACHHRLRYDPALERRPMPRGVPGRPPLARWPQRGALLAAEQAAGYD
                     AGQEADRWKTVRQPLAELEAAVLATPFGDPASMQMPAEALADALFQLAADASNTAFDA
                     AAAHRAAYSLTDATHLETADYTSARQTAWLLRQLFAHQGNEDARRLFLDKGEDYLHLQ
                     LPSKQQGSVIGNLYRWLPAAAAYDPMRFRDELGVLRKSMPRDP"
     gene            4612..>5609
                     /locus_tag="VFB80_18625"
     CDS             4612..>5609
                     /locus_tag="VFB80_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35855.1"
                     /translation="MSAGCDTTQLAQQVAASLDTHESLIDQLLTCEEAYNDVLAGVTD
                     ANSAREATARIKGLAEQYRGLAERHLALMEKSPGDPQRLNELYGERYQKMLARQIEER
                     VRIGRDRDISRNFDELEWSITKSEYDRKKQLIAYGIKLDANGKAIAPAGPPGPPPPPS
                     TASAPTSDIPPSAPPVAATNSPLDDDPKTAAAQDTAAAPTPAADAPPSAAEQPAESRP
                     PGRGFGGGFGSGFGAPGPIRPPFRPGPPPELTTTAVTIILLRRPADGGQKLERIIGQL
                     GDFKRQATQTTNSSFRMTVAPVGDLREFAKQLTWAKIASLDEDARTITIEIDPAKYP"
BASE COUNT          972 a         2046 c         1777 g          814 t
ORIGIN      
        1 gcggcagcgc caatggcacc aaccgcgcca gcccgggcgg acccaacatg aacgccctcg
       61 agcggctgct gcaaaaccgc ggctcctcgg gtgggaacaa tcccgccggc cgaatcatcg
      121 acggcgccgc tgcgggccgc ccccgcggcg acacgctggc caattcgctc cgcttaggca
      181 accgcacgtc ggccggcgac ctgggtcagt tcctgggcct caatcgccgg cccgcgggca
      241 gcgacctggg caacctgcgg ggcaatctcg cccgcaacgg cgtggcgggc gatctcgccg
      301 gcagcgattc tgtcggcggg ctgcgcggcc gcggactcgg ccggctgaac ctgaacgatt
      361 cgcaggtcaa cctgatcaag agcaacgtca acacggcgtt ccgcaacgaa ctgaacgtca
      421 gcaaccggat caacaacttc aacaacctga atatcaacaa cttccagcgc ttcaacaaca
      481 actacttcct caacaacccg gcccgggccc attactggaa caactggtgc ggcggcatcg
      541 ccaatcactg caacttcaac cggttctacg gctgcttcac cccgcgcttc tgggcgacga
      601 acttttgcta cttcccctgg cggagctcgt attactggtg gggcgcccgg ccctggagct
      661 attggtacgg ctgcccgacc tggtccacct tctgcagctt cttccccagc tacggctgga
      721 gccagcctta ttactacgac tacggcgtcg gcggcaacgt ggtctatagc gacggcaacg
      781 tccttgtgaa cgggtcgcag gtggcgaccg cggccgatta cgccgcgtcg gccgcggagc
      841 tcgctacgca gccggtgccg gtcaaccccg atcagccgac cgattggctg ccgctgggga
      901 cgttcgccct ctcgaccagc gagaccgaca aggacccgag ccgtgtggtg cagctggctg
      961 tcgataagga cggcaacatc aacggcacga tggtcaaccg gaccaccaac cagacctacc
     1021 cggtccaggg ccgcgtcgat aaagagacgc agcgcgtggc cttcacgatc ggtggcagcc
     1081 aggaagttgt gctcgagacc ggcctctaca acctgaccca gcagcaaact ccgctgctcg
     1141 ttcacggcgg cggccgcgag gaaacgtacc tgctctaccg gttggaagcc cccaagaccg
     1201 agagcactgc tccgcccgca ccggcggcga acccgccgct gttgccgtaa gccttccgca
     1261 gccgattgag caccaatcga acggcacagc gcagcccgcc gcaagaattg cggtgcgtta
     1321 acatgctgcc ggcgcattcc ggcgacgcgg aatgcgccgg cagtgcgctg tttatggaat
     1381 gcgctgttta cggaacgcgc gtgaatcgct gccccccctt cctgctgctg gccctctggc
     1441 ccgcatggat gctcgcggcg gacccgtggg ccgccagcga acccgatccg tgcctcctcg
     1501 tcggcacact ctcgtgcgcc tcggccagct gccacggccg ccccgagccc gcaagtaggc
     1561 cccttctgcc ggaaggggcc ttcggggagc gcagcgacca ggaacatcgc cgagattcga
     1621 cgtcaccgcg ccccccaggt cccgcccggc agacgggacc tacaagcggg gccgcaacca
     1681 tcaaccagca ggaatacctg cacttcctcg ccggcgatcc gcacgccgag gccgcgcggc
     1741 gaatgaccga gccgcggtat cgggaggtcc tgcggcgggc cagccagtac gacccggctg
     1801 ccgccacgcg ctgcgccccg tgccacgatc cgctcggcca cgcgagcgaa tcaagccacc
     1861 agtcgctggg acgcggcatc ggctgcgaat cgtgccacgg cgcggcccgc gactggctgg
     1921 cggtgcacta cgagcaggac gtcagccgcg agcggctcgt caaactcggc ctgatcgata
     1981 ctaggcaagt gctcgcgcga gcgggggtct gtgcctcgtg ccacgtcggt tctccaggcc
     2041 gcgacgtgaa ccacgacctg ttggccgccg gccatccgcc gctcttgttc gagctggcct
     2101 cgcaccaggc gctgatcgag cgcaagcact gggacgatac cgcccggcgc gagcgcgagc
     2161 cgaactacga ggtgcaactc tgggccgccg gccaaatcgc ctcggccgac gcggcgctct
     2221 cgctgctcgc cgctcgcgcg gagcaggcga gcaagtccgg gccctggccc gagttcgccg
     2281 agtccgactg ccgctcctgc caccggccct tgcgcgcgga atccggcagc tcaagcggtc
     2341 taccggcctg gcagtcctgg aacaccgcag gcatctcgcc gctcggttcg ctcgacgtat
     2401 tgcggcacga aatgcagcgt tcgctcgtgc cgcaggcggc ccaagttgcc gggctggccg
     2461 cggcggcccg caccgacctg cggcagagcg tgcggctgtc gccggaaggc gtcctcctat
     2521 gcgctaacgg ccggccgctg gatgccgccg cggtcctgga gcgactggat tctgcccagc
     2581 cgccgaactt tgaccgcgcg tgccaggagc tcgccgcgct gctggcggcc gggcgctcgc
     2641 tgggtgcgcc gagcgacctc gcgcggctgc gccggatcgc cgccgagctt cgtcgcgatg
     2701 cgccgatcac cgaaatcgcg gccaggctgg ctgccgcccg ccgcgaaatc agcgacaatc
     2761 tgccggcgag gcaaccatga ctctgtgccg cgcgctcgtc gctgtttgct gcgcctgcct
     2821 ggccctcgcg caggccaggg ctcaatatct cactacggag tccggctgcc tgagctgcca
     2881 taaagtgccg ccgcctgaca acctgttctg ccgggcggtt ccggtggagg tctgggctca
     2941 gaccgacaag cacagccggg cgttccacct gctgcacgag accgacccgg ccgatcctgc
     3001 caagggcgat gcgaagcgga cgctggtcaa gcaaatcttg ggattcgacc tggcaggcgc
     3061 attcgacggt ccgaactacc agcggctgaa ggccggcggc gacgccgaaa ccacccgcaa
     3121 agtcgcggtc gtcaagtcgt gcctgcgttg ccacgccacc tggccgattg cggccgacga
     3181 gcgctatccg accaggccgc cagcgccttt ggcgctcggc gtctcctgcc aggcctgcca
     3241 cgggccaagc gcccggtggg acctgccgca ccgggagcag gtctggcgga cggtcacacc
     3301 cgcgggcaag gccgagctgg gctttaccga cgtgcggacg acagccgcca aagcggggct
     3361 ctgcacctcg tgccacattg gcaacctggc cgagggcaag ttcgtcaagc acgagtggta
     3421 cgccgcgggc catccacctc tgccgagctt cgagctggcc acgttcgagg cggaaatgcc
     3481 gatgcactgg atcccgctgg cgaaaaaacc gaagttcgag tttcgcgacg accgcccggc
     3541 ggatgagggc gcgctggcgg accagctgga tacgctgaag cgcgccggcg tgcccgccgc
     3601 ggcgatcaag ccaacgtatc gcgcggcgaa ttttccggac gccgccgcca agggcctcga
     3661 tccgacgcgc gacctgccgc aggccagaag cgccgtcgtc gccggcgcgg cgacgcttga
     3721 gacctacgcc cgcctcgtcg gcgaatactc ccgcaaaagc gccgcgggcg agcccggttt
     3781 cgcctggccc gagctggcgc tctacgactg cgccgcctgt caccaccggc tgcggtacga
     3841 ccccgcgcta gagcggcggc cgatgccgcg gggcgttccc ggccggccgc cgctggctcg
     3901 ctggccgcag cgcggagcgc tgctcgccgc cgagcaggcc gccggctatg acgccgggca
     3961 agaggcagat cgctggaaga ccgtgcggca gccgctggcg gagctggaag cggccgttct
     4021 ggccactccc tttggcgatc ccgcgtcgat gcagatgccg gccgaggcgc tagctgacgc
     4081 cctgtttcag ctcgccgccg acgcgagcaa cacggccttc gatgccgcgg ccgcccaccg
     4141 cgccgcgtac tcgctcaccg acgccaccca cctcgaaacc gctgactaca cttccgcccg
     4201 ccagaccgcc tggctcctcc gccagctctt cgcccatcaa ggcaatgaag acgcccggcg
     4261 actgttcctc gacaagggcg aagattacct tcacctccag cttcccagca aacagcaagg
     4321 cagcgtcatc ggcaatctct accgctggct ccccgccgcc gcggcctacg atcccatgcg
     4381 gtttcgagac gagttgggcg ttctgcggaa gagcatgccg cgcgatccgt gatgcggcgc
     4441 gggtaatgct ctcgctccgc gagacagcgt attgaaagac tttgctggtg gcagaatgta
     4501 ctatactgtc gccgcagtcc ggtctttcga ttcaaagggc tgtctccgat tctggctgca
     4561 atgaacatgc gaatctccct ccgtaatgct agctgggctg ctctggtcgt cttgtcagca
     4621 ggctgcgata cgacccagtt ggcgcagcaa gtggccgcgt cgctggatac gcacgagtcg
     4681 ctgatcgatc agctgctcac ctgcgaagag gcctacaacg acgtcctggc cggcgtcacc
     4741 gatgccaact cggcccgcga agcaacggcc cgcatcaaag gactggccga gcaataccgc
     4801 gggctggccg agcggcacct ggcgctgatg gagaagtcgc ccggcgatcc ccagcgcctg
     4861 aacgagctct acggcgagcg gtaccagaag atgctggccc ggcaaatcga agaacgcgtg
     4921 cggatcgggc gcgaccgaga catcagccgc aacttcgacg aactggagtg gtcgatcacg
     4981 aaatcggagt acgaccgcaa gaagcagctg atcgcttacg gaatcaagct cgacgccaat
     5041 gggaaagcga tcgcgcccgc tggcccaccg ggaccgccgc cgcccccgtc caccgcgtcc
     5101 gcgccgactt ccgacatacc gccttcagct ccgccggtcg ctgcgacaaa cagtccgctc
     5161 gacgatgacc cgaaaactgc cgcagcacaa gacaccgccg ctgcgcccac gccggcagcc
     5221 gacgcgccgc cctccgcagc ggagcagccg gccgagtcga ggccgccagg taggggcttt
     5281 ggcgggggct ttggcagcgg ctttggcgct ccgggaccga tccgcccgcc attccggcct
     5341 ggcccgccgc ccgagctcac gaccacggcg gtgacgatca tcctgctgcg caggccggcc
     5401 gacggcggcc agaagctgga gagaatcatc ggccagctcg gcgacttcaa acgtcaagcc
     5461 acgcagacca ccaatagcag cttccgcatg accgtcgccc ccgtcggcga cctccgcgag
     5521 ttcgccaaac agctcacctg ggcgaaaatc gccagcctcg acgaagacgc ccgcacgatc
     5581 accatcgaga tcgatcctgc caaataccc
//
LOCUS       DASRJB010000701         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2461283, whole genome shotgun sequence.
ACCESSION   DASRJB010000701 DASRJB010000000
VERSION     DASRJB010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461283"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..621
                     /locus_tag="VFB80_18630"
     CDS             325..621
                     /locus_tag="VFB80_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35856.1"
                     /translation="MSRHETVLSTIPASETERLLVVLIQSPESGSRMELRQQSFSNGI
                     GWFTQSSVQLEPGQVAALRNSLGATGRPARPLPREFSKIPAAAWQPKVVHADSA"
     gene            722..1534
                     /locus_tag="VFB80_18635"
     CDS             722..1534
                     /locus_tag="VFB80_18635"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HZN35857.1"
                     /translation="MSTQLLSPDVREFTARALLVGQRIDLRALGSTQRLGGGPLALSV
                     RGGGAAFLFRYGAVVLFDVQPLEQDEFLRQLRPRIEQPTSEPDIEQVTVRIDPDTKEV
                     LEGATVLLKDTSSERLEIVADVLSKSVVLARYESTIAQTFDRIEPFAAELQKERTGSR
                     MARELLAHLGSALIIEQQMVGRVQIDDTPELLWDRPDLERLFARLRDEFEIVERFTAL
                     NRKLELVSRTVETALGLIQARRSLRVEWYVVILIVFEILLTLYQMFWLGKAH"
     gene            1645..2721
                     /locus_tag="VFB80_18640"
     CDS             1645..2721
                     /locus_tag="VFB80_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HZN35858.1"
                     /translation="MPRRIIILTEGHTEPHAGKTASCIIRYRGEDVVALLDGTQKGKT
                     SGDLLGVGKVPIVGRLDDVPEANTLLLGIAPPGGKIPPPWRAIILDAIARRKMDVISG
                     LHDFIGDDPEFSKAAKASGVAIQDVRKNSEKSIARRKGLRPDCLRVHAVGHDCSIGKM
                     VVSVEVTNGLKKRGHDAKFIATGQTGIMVEGDGLPIDCIVADFVSGAAEKLVLENQHH
                     EILVVEGQGSLVHPSYSGVTLSLLHGCAPQALIMCYEVGRERVTGVESVEIPSLNEII
                     RMYELMSNVHQPCKIIGIGINGRRLNKDEAARERARVKAEFGLPACDVIRDGPDELVE
                     AVLAFKNSTGWHSPVDEGPNHKHQ"
     gene            2788..>3092
                     /locus_tag="VFB80_18645"
     CDS             2788..>3092
                     /locus_tag="VFB80_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2993c-like domain-containing protein"
                     /protein_id="HZN35859.1"
                     /translation="MRLARFSHNGRSQVGFYDDKFVVPLAAAAAAYANATHEKLEPPA
                     GDSLLDLLPPDGKQFAAAKKIADWAARNDGGVPAAARLALEAVELHVPIPRPNKLLLL
                     "
BASE COUNT          580 a         1004 c          986 g          522 t
ORIGIN      
        1 caagcaatgg tacgggctat cgtcaccccc ggtgttcgga cgatgcgcgg acgttgtaat
       61 agttgtgtaa ggccggggag attcctccat aactcattga tgcaaaagca tttgcgacgg
      121 agagcgcgtg gggccagttg aggcgaagaa agggcagaat cccgcacttc tgatcttgtg
      181 ggcagcggtg cgccctccgc cattgaccgg ttttgcgggg caagccgaag gagacggttg
      241 acaagtgaac ggctattcgt agaatcaggt gattgagatt gaatctcaat taagtcgctt
      301 tcctgagttt ggctgcccgc cgctatgtcc cgccacgaga cggtcctcag caccatccct
      361 gcctccgaaa cggaacgcct gctcgttgtt ctgattcaat cgccggaatc gggcagccgc
      421 atggaattgc ggcagcagtc gttcagcaac ggcatcggct ggttcacgca aagctcggtg
      481 caattggaac caggccaggt cgccgcgctg cggaattctc tcggcgctac gggacgcccg
      541 gcgaggccgt tgccgcgcga gttctcaaaa atccccgccg ccgcgtggca gccgaaggtg
      601 gtccacgccg attcggcgta gccgacgcag cttgcgtcgc gtacgcggac gctggcagcg
      661 tccgcaacgg acggcttgtg tgcctggcgg ccagcttgtg taatcacgct ggacgcaccc
      721 catgagcacg cagcttctca gtcccgatgt gcgcgaattc accgcgcggg cgctgctcgt
      781 cgggcagcga atcgacctgc gagcgctcgg ctcgacgcag cgtctgggcg gcggaccctt
      841 ggcgctgagc gtccgcggcg gcggggcggc atttctgttc cgttacgggg ccgtcgtgct
      901 gttcgacgtg cagccgctgg agcaggacga attcctgcgg caattgcgac cgcggatcga
      961 gcagccgacg agcgagccgg acatcgagca ggttaccgtg cggatcgatc cggacaccaa
     1021 ggaagtgctc gaaggagcga cggtgctgct caaggacacg tcgagcgagc ggctggaaat
     1081 cgtggccgac gtgctcagca agagcgtcgt gctggcccgc tatgaatcga cgatcgccca
     1141 gacgttcgac cggatcgagc catttgccgc ggagctgcaa aaggagcgca ccggcagccg
     1201 gatggcccgc gagctgctgg cccacctggg cagcgcgctg atcatcgagc agcagatggt
     1261 gggccgcgtg cagatcgacg atacgccgga gctgctctgg gaccgccccg acctggagcg
     1321 cttgttcgct cgcctgcggg acgaattcga gatcgtggag cggttcaccg ccctcaaccg
     1381 caagctggag ctggtctcgc ggaccgtcga aaccgccctc ggcctgatcc aggcccgccg
     1441 ctcgctccgc gtcgagtggt acgtcgtgat cctgatcgtg ttcgagatcc tgctgacgct
     1501 gtatcagatg ttctggctgg ggaaggccca ctgaggggtt cagggttcag gtcagagccc
     1561 ttcagtcatt cacgacgttt caccgggaac ttaagatagt gtctcctgct gaaccccgaa
     1621 ccctgaaccc cgaaccctcc ccgcatgcct cgccgcatca tcatcctgac cgaaggccat
     1681 actgagccgc acgccggcaa aacggcgtcc tgcatcatcc gctaccgcgg cgaagacgtc
     1741 gtcgctctcc tcgacggcac gcagaagggg aagacgagcg gcgatttgct cggcgtgggc
     1801 aaggtcccga tcgtcggccg gctcgacgat gtgcccgagg cgaatacgct gctcctcggc
     1861 attgcgccgc ccggcggcaa gattccgccg ccctggcggg cgatcattct cgacgccatc
     1921 gcccgccgca agatggacgt gatcagcggc ctgcacgatt tcatcggcga cgacccggag
     1981 ttctcgaaag cggccaaggc cagcggcgtt gcgattcagg acgtgcgaaa gaacagcgaa
     2041 aagtccatcg ctcgccgcaa aggcctgcgg cccgattgcc ttcgcgtcca cgccgtcggc
     2101 cacgactgct cgatcggcaa aatggtcgtc tccgtggaag tcaccaatgg cctgaagaag
     2161 cgcggccacg acgccaagtt cattgccacg ggccagacgg gcatcatggt cgagggagac
     2221 ggcttgccga tcgactgcat cgtcgccgac ttcgtcagcg gtgcggccga gaagctggtc
     2281 ctggagaatc agcaccacga aatcctcgtc gtcgagggac agggcagcct cgtccatccc
     2341 tcctactccg gcgtgacgct cagcctgctg cacggctgcg ccccgcaggc cctcatcatg
     2401 tgctacgagg tcggccgcga gcgcgtcacc ggcgtcgaaa gcgtcgagat tccgtcgctg
     2461 aacgagatca tccggatgta cgagctgatg tcaaacgtcc atcagccgtg caagatcatc
     2521 ggcatcggga tcaacggccg ccgcctgaac aaggacgagg ccgcccgcga gcgggcccgc
     2581 gtcaaagccg agttcggcct gccggcctgc gacgtgatcc gcgacggccc cgacgaactg
     2641 gtcgaggccg tcctcgcgtt caaaaactcc accggttggc acagcccagt ggatgaagga
     2701 ccaaatcaca agcaccaatg accaaacaga ttccaagcac caatctcatt tgcgccacga
     2761 acctactctc cgaaccggag ccctcccatg cgtctagccc gcttctctca caacggtcgt
     2821 tcacaggtcg gcttctacga cgataagttc gtagtcccct tggccgccgc ggcggcggcg
     2881 tatgccaacg cgacgcacga aaagctggag ccgcccgccg gcgacagcct gctcgacctg
     2941 ctgccgccgg acggcaagca gttcgccgcg gcgaagaaga tcgccgactg ggcggcgcgg
     3001 aacgacggcg gcgtgccggc ggcggcccgg ctcgcgctgg aggcggtaga gttgcacgtg
     3061 ccgattccgc ggcccaacaa gctgctgctt ct
//
LOCUS       DASRJB010000702         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4152915, whole genome shotgun sequence.
ACCESSION   DASRJB010000702 DASRJB010000000
VERSION     DASRJB010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152915"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1558
                     /locus_tag="VFB80_18650"
     CDS             407..1558
                     /locus_tag="VFB80_18650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZN35860.1"
                     /translation="MQHFDFQPRTRIVFGPGKIESLGELASELGARRALVVSDSGVIA
                     AGHTGHGIAALERAGIDAHLFDAVHENPTTDDVAAGVKVARRYDPEILIGLGGGSSMD
                     CAKGINFVFTNGGEMKDYWGVGKALKAMLPMIAVPTTAGTGSETQSFALISDSKTHVK
                     MACGDKKASCRIALLDPELTVTQPPRVTALTGIDALSHALETFVTTRRNPASLAFSRE
                     AWRLLAGNFAAVIDQSDNIDARGSMLLGASFAGLAIENSMLGAAHALANPLTTLFDVP
                     HGQAVGVMLPHVIRFNAAQVENQYHDLLNESQNGSPLPKVEGGVEGLADFVGRMLTQA
                     GLATTLRPLGVDPLKLPQLATQAAKQWTATFNPRPVGEEELLALYQQAY"
     gene            1774..2847
                     /locus_tag="VFB80_18655"
     CDS             1774..2847
                     /locus_tag="VFB80_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN35861.1"
                     /translation="MATTQAAVATEQSWPVYRGDAQARGVAAAKLPEKLDVVWKITVP
                     GGAFEATPVIEGGVVYLADMDGKLYALNLETGKEVWTYKVDSGFIASPAIRGGLLFVG
                     DIDGKFYAIDIKTCKPKWTYEAGAEIDSAANFWKDNVLFGSQDSNLYCLNAASGKLVW
                     KHAIEDQIRCSPTVVGDRSFVAGCDSKFHIIDLVEGKEVGAVPIEAPTGVTPAALGDQ
                     VFFGTYAGTFFAVNWKDAKVSWKVEDKAGSQEMRSSPAVQDGMVIVGSRNRAVIAYDP
                     ATGNELWSFTTKQRVDSSPVIVGHRVFAGAADGRLYAIDLKQGTELWQYQATGGFTGS
                     PAVAGGKLVIATDRGVVYCFGGK"
     gene            2882..>3389
                     /locus_tag="VFB80_18660"
     CDS             2882..>3389
                     /locus_tag="VFB80_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN35862.1"
                     /translation="MTTEATKTEVGSYFISNYPPFSQWTAEQLPEIEAALHSPPRALA
                     DGGLRPTPLGLYLHIPFCRKRCKFCYFRVYTDKNSHDVERYVQALEKEIELVSQLPVM
                     DGRPFRFVYFGGGTPSFLSAKQLTSLVDRLRKHVNWSHAEEVTFECEPGTLSEPKIKT
                     LKELGVTRL"
BASE COUNT          650 a         1044 c         1104 g          591 t
ORIGIN      
        1 caaacgcctc ctggagcgcc tcttcggcca ggtcaaagtc gcccagcagg cgaatcagcg
       61 ttgccagcgc ccgccgggat tcggcgcgat agatcgcatc gaccgcctga ctggcgacgg
      121 cttggctcat gcgacatttc caactgaaat cgagacgtta gggcgccgct ggaagctaca
      181 ttttacgcgc gacgaaggct gccacacacg cctgcggctg cgacataatg acgagctgtt
      241 tttgtgccga aaagcggtcg ctgcggttta tccggcgagc gttcggcggt aaaatgcaag
      301 aattgcacga tcccgcctct cctgcggctg ctgcttcggg gcagcggccg tggttcgacc
      361 gcgcctggcg gctgaaccgc gccagccact ttccccccac cccggcatgc agcactttga
      421 ctttcagccg cgaacgcgga tcgtcttcgg tccgggaaag atcgaatcgc tgggcgaatt
      481 ggcctcggag ttgggggcgc ggcgggcgct ggtagtgagc gattcagggg tgatcgcagc
      541 ggggcataca ggccacggca tcgcggcgct ggagcgggcg gggatcgatg cgcacctctt
      601 tgacgcggtg cacgaaaacc cgacgacgga cgacgtggcc gccggcgtga aggttgcgcg
      661 gcggtatgac cccgaaattc tgatcggcct cggcggcggc tcgtcgatgg attgcgccaa
      721 gggaatcaac ttcgtcttca ccaacggcgg cgagatgaag gactattggg gcgtcggcaa
      781 ggcgctcaag gcgatgctgc cgatgatcgc cgtgcccacc accgccggca cgggcagcga
      841 gacgcagtcg ttcgcgttga tcagtgacag caagacgcac gtcaagatgg cgtgcggcga
      901 caaaaaagcg tcgtgccgca tcgcgctgct cgatcctgaa ttgaccgtca cccagccgcc
      961 gcgggtgacg gcgctgaccg gcatcgacgc cctgtcgcac gccctggaga cgttcgtcac
     1021 gacgcgccgc aatccggcct cgctcgcgtt cagccgcgag gcctggcggc tgttggccgg
     1081 caacttcgca gcagtcatcg accaatccga caacatcgat gcccgcggca gcatgctgct
     1141 gggggcgtcg ttcgccggcc tggcgatcga aaactcgatg ctcggcgccg cccacgcgct
     1201 ggccaatccg ctgacgacct tgttcgatgt gccgcacggg caggcagtgg gcgtgatgct
     1261 gccgcacgtc attcggttca atgcggcgca ggtcgaaaac cagtatcacg acctgctcaa
     1321 cgagtcgcaa aacggcagcc cgctgccgaa ggtggagggc ggtgtagaag ggctggcgga
     1381 ctttgtcggc cggatgctga cgcaggccgg gctggccacg acgctgcggc cgctgggcgt
     1441 cgatccgctg aagctgccgc agctggcgac gcaagccgcg aagcagtgga ctgcgacctt
     1501 caacccccga cccgtcggcg aagaagaatt gctggccctg tatcagcagg cgtactaata
     1561 gaatggaggg gaggacggac gatgaagagt aatggtcgat gcactctcga tacgatgaag
     1621 actaggaatc tcacacagag acacagaggc acagagaaag agcattcaag ttctctctgt
     1681 gcctctgtgc ctctgtgtga caaccttgcc gcgatccttt tcgttctatt ggccggttgc
     1741 ctgctctgcg tagatgctca agataagccg caggtggcca caacccaggc tgctgttgcg
     1801 accgaacaaa gctggcccgt ctatcgcggc gatgcccagg cgcggggcgt tgccgcggcc
     1861 aaactgcctg agaagctcga cgtcgtctgg aaaatcacgg tgcccggcgg cgcgttcgag
     1921 gccacgccag taatcgaagg gggcgtcgtc tatctggccg acatggacgg caagctttac
     1981 gcgctgaatc tggagacggg caaggaagtc tggacgtaca aggtggacag cggcttcatc
     2041 gcgtcgcctg cgattcgcgg cggcctgctc ttcgtcggcg atatcgacgg caagttctac
     2101 gccatcgaca tcaagacctg caaaccgaag tggacgtacg aagccggggc ggagatcgat
     2161 tcagcggcca acttctggaa ggacaacgtg ctgttcgggt cgcaagactc aaacttgtat
     2221 tgcctgaacg ccgccagcgg caagctcgtc tggaagcacg ccatcgagga ccagattcgc
     2281 tgctcgccca ccgtcgtcgg tgaccgctcg ttcgtcgccg gctgcgacag caagttccat
     2341 atcatcgacc tggtcgaagg caaagaagtg ggcgccgtgc cgatcgaagc cccgacgggt
     2401 gtcactccag cggcgctggg cgatcaggtc ttcttcggga cctatgccgg cacttttttt
     2461 gccgtcaact ggaaagacgc caaggtttcc tggaaggtcg aagacaaggc cggctcgcag
     2521 gagatgcgca gctcgcccgc agtgcaggac ggcatggtca tcgtcggcag ccgcaaccgc
     2581 gcggtgatcg cctacgatcc ggcgaccggc aatgaactct ggagcttcac gaccaagcaa
     2641 cgggtggaca gctcgccggt gatcgtcggc catcgagtgt tcgccggcgc ggccgacgga
     2701 cggctgtacg cgatcgacct gaagcagggg accgaattgt ggcagtacca ggccaccggc
     2761 ggcttcaccg gttcccccgc ggtcgccggc ggcaagctgg tgatcgcgac cgaccggggc
     2821 gtggtgtatt gctttggggg gaagtagatc aagggatgat gcgcagacga ggtttagctt
     2881 agtgacgacc gaagcgacca agaccgaagt tggcagctac ttcatctcca actatccgcc
     2941 gttctcgcag tggacggcgg agcagttgcc ggagattgag gcggctttgc actcgccgcc
     3001 gcgggccctt gctgatggcg ggctgcgccc cacgccgctc ggcctgtacc tgcacattcc
     3061 gttctgccgc aagcgctgta agttctgcta ctttcgcgtg tataccgaca agaactcgca
     3121 cgacgtcgaa cgctacgtgc aggcgctcga aaaggagatc gagctggtca gccagctgcc
     3181 ggtgatggac ggccggccgt tccgcttcgt ctatttcggc ggcggcacgc cttcgttcct
     3241 ctcggccaag cagctcacct cgctcgtcga tcgcctccgc aagcacgtca actggagcca
     3301 cgccgaggaa gtcaccttcg aatgcgagcc gggcacgctc tcagagccga aaatcaagac
     3361 gctgaaagag ctcggcgtca cgcggctga
//
LOCUS       DASRJB010000703        10356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1617150, whole genome shotgun sequence.
ACCESSION   DASRJB010000703 DASRJB010000000
VERSION     DASRJB010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617150"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..925)
                     /locus_tag="VFB80_18665"
     CDS             complement(119..925)
                     /locus_tag="VFB80_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN35863.1"
                     /translation="MARDVLTTDIRGQFILLGTGTSVGVPSIGCPCEVCASADARDKR
                     LRCAALVGLPQGNLLIDTPPDVRQQLLREKVGLVHAVLYTHEHADHIFGLDDLRLLPF
                     YLGHAVPLYCEAIVEDRIRKSFDYAFSDVEQTHTGAVPQLVFHRIGLAPIELLGATIT
                     PLRLKHGPRFDVLGFRIGNVAYCTDTSGIPPESLALLEGLDVLVLDALRPRPHITHLS
                     LDEAIALAGQLKPKRTIFTHMSHELGHAQTNARLPVGMELGYDGMRLPLT"
     gene            1381..2199
                     /gene="larE"
                     /locus_tag="VFB80_18670"
     CDS             1381..2199
                     /gene="larE"
                     /locus_tag="VFB80_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZN35864.1"
                     /translation="MTSLGAKRDALLATLTKLESCAVAYSGGVDSAVVAQAAHLALGE
                     RAVAVTGASASLASGELELAEQVARQIGIRHVVVATDELANANYTRNAPDRCYHCKTE
                     LYTQLERLLPQLGVKAIVNGANADDLGDYRPGMQAAAEHRIHSPLADCGLTKADVREL
                     AAAWNLPVWDKPATPCLSSRIAYGQAVTPERLAMIDAAEQYLRGRGLANLRVRYHEGD
                     LARIETPLADLPRLCEADFRQELTVELRRLGFKFVTLDLEGFRSGSLNQLVQIG"
     gene            complement(2196..4058)
                     /locus_tag="VFB80_18675"
     CDS             complement(2196..4058)
                     /locus_tag="VFB80_18675"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN35865.1"
                     /translation="MRLVLLVLAATWLIRIPLAAAATPEQRAEMAAVGTLLTKAGNLY
                     KNSKFKEAGEAVKEVQSRLEKLAEGADPQMGQQLEAMHKRLINAHALLELEGISLAAL
                     KPLTPPKAAGKPGDPPAVGTVSFVKQVAPILNARCGGCHVRNARGMFSMATYESLMKG
                     PPPGKVVFPGNVAGSDLIVKVQDKEMPPNGAGIPDPELAMLKKWVEEGAKFDGPDPMA
                     MLTSLVAGAPAPAPAPMITVQQATGNETVSFSQDIAPVLVANCINCHGADRPRNNFSL
                     NTPETLLRGGDRGEPVLPGRAADSLLVKKLKGTADGARMPMGGSPLDDTVMAKIEKWI
                     AEGAKFDSSNPKQPIEEIAAIARAKKATHAELSADRAKLADENWQLGMPGMAPFRTES
                     ENFLLLGSVNEETLGEIAKQAEALAPKIMDIFKGPAGQPLVKGKLTLFVFAEKYDYGE
                     FGKMVEKRPLPPAWRGHSRYTVVDAYGAVLNPRSSDYSLDALLAQQIAALYIAAQGQG
                     VPHWFAEGCGRVVASRLAPASDKRVAQWNDELPGVMGMMTAPDDFLTGKLPPEEADIC
                     SYNFAKFLMSDGRRFRILMDGLKKGGDFAKTFEGAYGPPAQVAAAWARNPPKGR"
     gene            complement(4192..4890)
                     /locus_tag="VFB80_18680"
     CDS             complement(4192..4890)
                     /locus_tag="VFB80_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35866.1"
                     /translation="MRHIDQSLKAISRAHNNSARSSRKFVTLGKRARISSTALRGPYL
                     PPENWHEPRETPQGKFCVVMQPAGAGFRHVLTKDDVRERLARLPEWMVEPLQVVQFSQ
                     MTQKKRRSPCYGMQWGQTIYLYPIEESRIEVFHAPPKPAQRIEAAMYGARWQAADAGR
                     WHLVWTEDAIRDFYLNNVLIHELGHILDSRNTSHRDRERYAEWFALEMGYKASRRADL
                     AERAAAKYVVRRHG"
     gene            complement(5081..6949)
                     /locus_tag="VFB80_18685"
     CDS             complement(5081..6949)
                     /locus_tag="VFB80_18685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35867.1"
                     /translation="MVKLTVDSFLDLVEKSKLVEQGALQKALAEFRGKAGDPNDDGEQ
                     LARFLIQKNLLSTWHVEKLLDKKYRGFFLGKYKLLRHIGSGGMSTVYLAEHTLMHRQR
                     AIKVLPRKRINDSSYLARFHLEAQATAQLDHPNIVRCYDVDNEEDTHYIVMEYIEGKD
                     LNTIAKQEGPLPLELACNYIAQAAEGLHHAHQNGLIHRDVKPANLLVDRKGVVKILDL
                     GLALFSDNERASLTVEHNENVLGTADYLAPEQAVNSHKVDHRADIYGLGCSLYYVLTG
                     HPPFPEGTLAQRIAKHQTQMPDDIRKDRPDCPRDLADICVKMMQKRPEKRYANMREVA
                     AALEHWLQAHGYKYEPGSGDAAVKAALLTNNPTAARGPGSSASASGGSLGGSKSQGRL
                     MPARRPKASPNEDTVYDKARDTKKDLNKAGQSDPGSDKGSGKRVGKVLPKAKALDDPG
                     SDKKTASGSQFVLQPESKAPGKAASGAMPTVNINTNPNRSGAVPVVKDRGRVSGAMPV
                     AKAAGGAAASNVAGAAPAGGAKTIAVAPWVLITAAASAAVVILGLLIGLLIASGAFSP
                     AKTPAPPAPKTTSTAHSWNTASQRLVTRQRPIDDAAATVLAPQSNQHAAIARPAAF"
     gene            7297..7530
                     /locus_tag="VFB80_18690"
     CDS             7297..7530
                     /locus_tag="VFB80_18690"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZN35868.1"
                     /translation="MRRVVLYTRQGCHLCDDARSLLERYGLKPSLVDIDADPQLRVRY
                     TECVPVVEIDGRERFRGRVNEVLLRRLLAGQDS"
     gene            7532..8206
                     /locus_tag="VFB80_18695"
     CDS             7532..8206
                     /locus_tag="VFB80_18695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN35869.1"
                     /translation="MSTLLGIFAKHWTPGRVKTRLAEAIGDARAAEVHRLFVTAIAQR
                     MGAASDERILAIDPPESEHEFGRALGDSWQVEPQAAGDLGERMAAFFAGGLARAGLVV
                     LIGSDSPDLPADYIADAFEALREVDVVLGPAADGGYYLVGAARSVPPIFTGISWSTAD
                     VWPQTVRRLTQAGCQWRSLPQWYDVDTFDDLRQLRFRLKDASSADRSLHALACRLSEL
                     FADSPP"
     gene            8214..9329
                     /gene="thiO"
                     /locus_tag="VFB80_18700"
     CDS             8214..9329
                     /gene="thiO"
                     /locus_tag="VFB80_18700"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HZN35870.1"
                     /translation="MHDVLIIGGGVIGLSLAWDLARHGRSVQVIDQGEPGREASWAGA
                     GILPAAVRRTDQHPYEQLYGLAGELHSDWARDLHAATGIDNGYRRCGGLYLARTPGET
                     AALAAWAAALRDEGIEVQQLSADRLAGLEPKLAVAQQSGRSAAPCFHVPGEAQLRNPR
                     HLQALSLACRAAGVTIASNTAASGAVITAGQVEAIETSAGPLRAGQYCFTAGAWTGQL
                     LSQLGISVGIVPIRGQMVLFRCAEPPVRHIINEGSRYLVPRDDGRLLAGSTEEEVGFD
                     KRTTDDAIAELTRFARSLIPALAGAEIEQTWAGLRPGSFDGLPYLGRLPGLPNAFIAS
                     GHFRSGLSLSPATAVVMSQLIRDEPTEIDLAPFRVGR"
     gene            complement(9350..10180)
                     /locus_tag="VFB80_18705"
     CDS             complement(9350..10180)
                     /locus_tag="VFB80_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN35871.1"
                     /translation="MSPRLLLAMHVLALAVCAGCQQGPFAAATAQPAATSTAYQQQTQ
                     SLTAQVQDLNRRVSQLDVNNADLHRQLAQHEQAKQAAENQVRLLQQQIDVAARQARES
                     LLAKQEADKQVTALQASTRARGGATITANSSIRQSLATIAIPGLDIRQDGAVIRIEIP
                     GDKLFLPGASQIQADGYRILDEVAAAVQRSYPRQRIVVEAHTDDALASQPTAAHSLTT
                     AQAQAVFTQLVQRGRVPARQLSILAMGDNHPLASNATPVGKAKNRRVEIVVYPDTLDG
                     "
BASE COUNT         1660 a         3318 c         3459 g         1919 t
ORIGIN      
        1 gccagcaact acaacagcaa gccgttcgcc ccggaggtgc tgattcaggg cggccagccg
       61 cacctgatcc gcgctcggca atcgctggac gacttgattc gcggagaggc aattcccgtc
      121 aagtgagagg taaccgcatg ccgtcgtagc caagctccat cccgaccggg agtcgggcgt
      181 tggtctgggc gtggccgagt tcgtgcgaca tgtgagtgaa gatcgtccgt ttgggcttga
      241 gctggccggc cagggcgatg gcttcgtcga gcgagaggtg cgtgatgtgc ggccgcggcc
      301 tgagcgcgtc gagtacgagc acgtcaagac cttccagcag cgcgaggctc tccggcggaa
      361 tgccgctggt gtcggtgcaa taggccacgt tcccgatgcg gaagcccagc acgtcgaacc
      421 gcggcccgtg cttgagccgc agcggcgtga ttgtcgctcc gagcagctca atcggcgcga
      481 ggccgatgcg gtgaaacacc agttgcggca ccgcgcccgt gtgcgtctgc tccacgtcgg
      541 agaaggcgta gtcgaacgac ttgcggatgc ggtcctcgac gatcgcctcg caatacaagg
      601 gcaccgcgtg gcccaaatag aacggcagca gccgcaggtc gtcgaggccg aagatgtggt
      661 cggcgtgctc gtgagtgtag agcacggcgt gaacgaggcc gactttttcg cgcaggagct
      721 gctgccgcac atcgggcggc gtgtcgatca gcaagttccc ttgcggcagg ccgaccagcg
      781 ccgcgcaccg cagccgttta tcgcgcgcgt ccgcgctggc gcagacctcg caggggcagc
      841 cgatcgacgg cacgcccacg ctggtgcccg ttcccagcag gatgaactgc ccgcggatgt
      901 cggtggtgag aacgtcgcga gccaattgtc agtggtcaat tgtcagtggt cagtgggagg
      961 gagacggtga gcaatggaat ataccaccag cgctcaggct ggttcgcaac tgacaactga
     1021 cggctgacta ctgaccactg acaactgact gtctgattcg cccttgccac gggcgagtcc
     1081 tcccactaac ttcccgccaa cgacgacgct ctgccatgct cgtgctgact tgccacatgc
     1141 gatcggagcc gataagcagc cgcaggggcg ccgaatctgc ctgacgggcg cgtccacccg
     1201 acgttgtcgg ttaacgcacc ccagggctgt ggcgtccacc ttagacttgc gggctctttc
     1261 catcgcgagt ccacggcatt gcggtggagc attcgtcttt tggaaagggc tagggctagg
     1321 ggctaggggc taggggctag gggctagggc tggggtcgat ggacggcgta ttatggagac
     1381 atgacctcgc tcggtgccaa acgcgatgcc ctgctggcca cactgacgaa gctcgagagc
     1441 tgcgcggtgg cctattccgg aggcgtcgat agcgcggtgg tggcccaggc ggcgcacctg
     1501 gccctcggcg agcgggcggt agccgtgacc ggcgccagtg ccagcctggc cagcggcgaa
     1561 ctcgaactgg ccgagcaagt cgcccggcag atcggcatcc gccacgtcgt cgtggccacc
     1621 gacgagctcg ctaacgcgaa ctacacccgc aacgctcccg accgttgcta ccactgcaag
     1681 acggagctgt acacgcagct cgagcggctg ctgccgcagc tcggagtgaa ggcgatcgtc
     1741 aacggcgcga acgccgacga cctgggcgac tatcgccccg gcatgcaggc ggccgctgag
     1801 catcggatcc acagcccgct ggccgactgc ggtctgacga aagccgacgt ccgcgagctg
     1861 gccgccgcgt ggaatttgcc cgtgtgggac aagccggcga ctccctgcct ctccagccgc
     1921 atcgcctacg gccaggccgt gacgccagag cgtctggcga tgatcgacgc cgcggagcaa
     1981 tacctccgcg gccgcgggct ggcgaacctg cgggtccgct atcacgaggg cgacctggcc
     2041 cggatcgaga cgccgctggc cgatctgccg cggctgtgcg aggcggactt tcgccaagaa
     2101 ctgactgtcg agctccgccg tctaggcttc aagttcgtga cgctcgacct ggaagggttc
     2161 cgcagcggca gcctgaacca gctcgtgcag attggctagc ggcccttcgg cgggttgcgg
     2221 gcccaggcgg cggcgacctg ggcgggcggc ccgtaggcgc cctcgaaggt cttggcgaag
     2281 tcgccgcctt ttttgaggcc gtccatcagg atgcggaacc gccgtccgtc ggacatcagg
     2341 aacttcgcga agttgtagct gcagatgtcg gcctcttcgg gcgggagctt gcccgtgagg
     2401 aagtcatccg gggcggtcat catgcccatg acgccgggca gctcgtcgtt ccactgggcg
     2461 acgcgcttgt cgctagcggg ggcgaggcgc gaagcgacga cgcggccgca gccttcggcg
     2521 aaccagtgcg gcacgccctg gccctgcgcg gcgatgtaga gggcggcgat ctgctgggcg
     2581 agcagcgcgt cgagcgaata gtcgctggag cgaggattca agactgcccc gtaggcatcc
     2641 accaccgtgt agcgagaatg cccgcgccaa gccggcggca acggccgctt ctcgaccatt
     2701 ttgccgaact cgccgtagtc gtatttctcg gcgaagacga acagggtcag tttgcccttt
     2761 accagcggct gcccggcggg gcctttgaag atgtccatga tcttgggagc cagcgcttcg
     2821 gcctgcttgg caatttcgcc cagcgtctcc tcgttcacgc tgcccagcag caggaagttc
     2881 tcggactcgg tgcgaaacgg ggccatcccc ggcatgccca gctgccagtt ctcgtctgcc
     2941 agcttggccc ggtcggcact caattcggcg tgcgtcgcct tcttggcccg ggcgatcgcg
     3001 gcgatctctt cgattggctg cttgggattg gacgaatcga acttcgcccc ttcggcgatc
     3061 cacttctcga tcttggccat gaccgtgtcg tcgagcggcg agccgcccat cggcatccgc
     3121 gcgccgtcgg ccgtaccttt gagcttcttc accagcaggc tgtcggccgc gcggcccggc
     3181 agcacgggct cgccgcggtc gccgccgcgg agcagggtct cgggcgtatt gaggctgaag
     3241 ttgttccgcg ggcgatccgc gccgtggcag ttgatgcagt tggcgacaag aaccggcgcg
     3301 atgtcctggg agaagctgac cgtttcattg cccgtggctt gctgcacggt gatcatcggc
     3361 gcgggggccg gcgcaggagc tccggcgacc agcgaggtga gcatcgccat cgggtctggg
     3421 ccgtcgaact tcgccccttc ctcgacccac ttcttcagca tggccagctc cggatcggga
     3481 atgccagcgc cgttcggagg catctccttg tcctggacct tgacgatcag gtcgctgccc
     3541 gcaacgtttc ccggaaagac gaccttgccc ggcggcggac ccttcatcag cgattcgtac
     3601 gtcgccatcg agaacatgcc gcgggcgttg cgcacgtggc agccgccgca gcgggcattc
     3661 aggatcggtg cgacctgctt gacgaagctg accgtgccca cagccggcgg atcgccgggc
     3721 ttgccggccg ctttgggagg cgtcaaaggc ttcagagcgg ccagcgaaat gccttccaat
     3781 tccagcaggg catgggcgtt gatcaaccgt ttgtgcatcg cttcgagctg ctggcccatc
     3841 tgcgggtcgg ccccttcggc gagcttctcc agccgcgact gcacctcttt gacggcttct
     3901 ccagcctcct tgaacttgct gttcttgtag aggttaccgg ccttggtcag cagggtgccg
     3961 acggctgcca tttcggcccg ctgctcggga gtggcggcgg cggccagggg gatgcgaatc
     4021 agccatgtgg cagcgagaac caatagaaca agacgcatcg aagaaggctc cgctcgcagc
     4081 cgggcttgcc gttgcaagtt catatcgcaa ctgaggttat ggcttcaccc tggcgcgcag
     4141 tactggctgc caatcttaaa cggacacaaa ggagaaaggt actcaatcga cctagccatg
     4201 ccgccgcacg acgtactttg cagccgcccg ttcggccaga tcggcccggc ggctggcctt
     4261 gtagcccatt tctagcgcga accactcggc gtaccgctcg cggtcgcggt ggctggtatt
     4321 gcggctgtcg aggatgtgcc ccaactcatg gatcagcacg ttgttgaggt agaagtcgcg
     4381 aatggcgtct tccgtccaga cgagatgcca gcggccggcg tcagcggcct gccagcgtgc
     4441 gccgtacatc gcggcttcga tccgctgcgc cggcttgggc ggcgcgtgga agacctcgat
     4501 tcgcgactcc tcgatcggat agaggtaaat cgtctgcccc cattgcatgc cgtaacaggg
     4561 cgaacgccgc tttttctgcg tcatctggct gaattgcacg acctgcagcg gctcaaccat
     4621 ccactcgggc agccgcgcga ggcgctcgcg cacgtcgtcc ttggtcagca cgtggcggaa
     4681 gccagccccc gccggctgca tgacgacaca gaacttgccc tgtggcgtct cgcggggctc
     4741 gtgccagttc tccggcggca ggtagggccc gcggagtgcg gtgctgctga ttcgcgcgcg
     4801 cttgccgagc gtgacgaact tccgcgagct gcgcgcggag ttgttgtgcg ctcgagagat
     4861 ggccttcagc gattgatcga tgtgccgcat gggtgcgtcc tctgccgaaa gcccctcggc
     4921 aggctacacc tatggtacga ccccatgcga tccgatttca agcgacgcct ataagtgcat
     4981 tgtcagaaag gacttagatc ctttcggcga cctggacgta acaggccggt gggggttttt
     5041 cggccgggat ttgccctgtc gaaaacgatc ccaaatcggc tcaaaacgcc gccggccgcg
     5101 cgattgcggc atgctgattg ctttgcggcg ccaagacggt cgctgcggca tcgtcgatgg
     5161 gccgttgacg cgtcaccagg cgctgactgg ccgtgttcca cgaatgagcc gtggaggttg
     5221 tcttcggcgc tgggggcgcc ggagttttgg cgggcgagaa agcaccgctg gcaatcagca
     5281 atccgatgag caatcccagg atgaccacgg cggcgctggc ggctgccgta atcaagaccc
     5341 acggcgcgac ggcgatggtc ttcgcacctc cagcgggagc ggcgccggcc acattacttg
     5401 cggccgctcc accagccgcc ttggcgacgg gcatcgcacc ggaaacccga ccccggtcct
     5461 taactactgg caccgcgccg gaccggttgg gattggtgtt gatgttgacg gtcggcatcg
     5521 cgccggacgc ggctttgccg ggtgccttgc tttcaggctg aagcacgaat tgggaccccg
     5581 atgcggtttt cttgtcgctg ccgggatcgt ccagagcctt agccttgggc agcaccttgc
     5641 cgacgcgctt gccgctccct ttatcagagc cgggatcgga ctggcccgcc ttgttgagat
     5701 ctttcttggt gtcgcgagcc ttgtcgtaaa cggtgtcttc gttcgggctc gccttgggac
     5761 gccgggcggg catgagcctg ccctgcgact tggagccgcc gagcgatccg cccgacgcgg
     5821 acgccgacga accaggcccc cgagcggcgg tcggattgtt cgtcagcagg gcggccttga
     5881 cggctgcatc gccgctgccc ggctcgtact tgtagccgtg ggcctgcagc cagtgctcga
     5941 gggcagcggc gacctcgcgc atgttggcgt atcgcttttc cggtcgtttt tgcatcatct
     6001 tgacgcagat gtcggccagg tcgcgcgggc aatcgggccg atccttgcgg atgtcgtcgg
     6061 gcatctgcgt ttgatgcttg gcgattcgct gggccagcgt gccctcggga aacggcgggt
     6121 ggccggtcag cacatagtag agcgagcagc ccaggccgta gatgtcggcc cggtgatcga
     6181 ccttgtgact gttgaccgcc tgctccgggg cgagatagtc ggccgtgccc agaacgtttt
     6241 cgttgtgctc gacggtcagc gacgcccgct cgttgtcgga aaacagggcc aaaccaaggt
     6301 cgaggatctt caccactccc ttgcgatcga cgagcaggtt ggcgggcttg acgtcgcggt
     6361 ggatcaggcc gttctggtgg gcgtggtgca agccctcggc agcctgggcg atgtagttgc
     6421 acgccagttc cagcggcagc ggcccttcct gcttggcgat cgtgttgagg tctttgccct
     6481 cgatgtactc cattacgatg tagtgcgtgt cttcctcgtt atcgacgtcg tagcaccgca
     6541 cgatgttcgg atggtcgagc tgcgccgtgg cttgggcttc caggtgaaag cgggccaggt
     6601 agctggagtc gttgatccgt ttcctcggca gcaccttgat cgcccgctgg cggtgcatca
     6661 gcgtatgctc ggccagatag accgtgctca tgccgccgct gccgatgtgc cgcagcagct
     6721 tgtacttgcc gagaaagaac ccccggtact tcttgtcgag cagcttctcg acgtgccacg
     6781 tcgaaagcag gttcttctga atgaggaatc tcgccagctg ctcgccgtcg tcgtttggat
     6841 cgccggcctt gccgcgaaat tcggccagcg ccttttgaag cgcgccctgc tcgacgagct
     6901 tgctcttctc gacgagatcc agaaagctat caaccgtgag tttcaccata tcgcgagggg
     6961 gacttgctgg cggctggcgc accggcaata ccgtccagtc tccccgctcc tgttactctc
     7021 acgagccgcg attttccaga ccccaagctc tacggtcagt gggacacgaa acgcccgacg
     7081 agctgacaaa aaaccaatcg caaaaaggtt acacccggtc cggctgcccg taaagcagca
     7141 ttccccaatt ttaacagcga ttgggccaaa gcaagcaaaa accgcgccca cctacaagag
     7201 aattggccaa tagcctgcgg cggcgacgct gacgacagcc agctccattc gtagaatgcc
     7261 ccattctggc cgtgccatgc caatccaggg ccaacgatgc ggcgagttgt cctttatacg
     7321 cgtcagggct gccatctctg cgacgacgcc cgctcactgc tggaacgcta cggcctaaag
     7381 ccgtcgctgg tcgatatcga cgcggatcca caactgcgcg tgcgatacac cgagtgcgtc
     7441 cccgtggtgg aaatcgacgg ccgcgagcgc ttccgcggac gcgtgaatga agtgctgctc
     7501 cgccggctgc tggccggcca ggattcgtag catgtccacg ctgctgggga tctttgccaa
     7561 acactggacc cccggccggg tgaagactcg cttggccgag gcgattggcg acgcgcgggc
     7621 ggccgaggtg caccggctgt tcgtgacggc gattgcccag cggatgggcg cggccagcga
     7681 cgagcggatt ctggccattg atccgcccga aagcgagcac gagtttggtc gggctctggg
     7741 tgacagctgg caagtggaac cgcaagccgc gggcgacctg ggcgagcgga tggcggcgtt
     7801 ctttgcgggc ggactggccc gcgcggggct cgtcgtcctg attggttcgg acagccccga
     7861 cctgccggcg gattacattg ccgacgcatt cgaggcgctg agggaggtgg acgtcgtgct
     7921 cggtccggcc gccgacggcg gctattatct cgtcggggcg gcgcgcagcg tgcccccgat
     7981 cttcaccggc atttcttgga gcaccgcgga cgtctggccg caaacggtcc ggcgactcac
     8041 gcaagccggc tgccagtggc gctcgcttcc gcagtggtac gatgtagata cgttcgacga
     8101 tttgcgccaa ttgcggtttc gcctgaaaga cgcctcatcc gctgatcgca gccttcacgc
     8161 gctcgcctgc cgcttgagcg aattatttgc cgactcgccg ccatagcttc gcgatgcacg
     8221 acgtactgat tatcggcggt ggagttattg gcctgtcgct cgcctgggac ctggcgcggc
     8281 acgggcgctc ggtgcaggtc atcgatcagg gcgagccggg acgtgaagcg agctgggccg
     8341 gtgctggcat cctgccggcc gcggtccgcc ggaccgacca gcatccctac gagcagctgt
     8401 acggcctggc cggcgagctg cactcggatt gggctcgcga cctgcatgcc gctaccggca
     8461 tcgacaatgg gtatcgccgc tgtggcggcc tgtacctggc ccgcacgccc ggcgagaccg
     8521 cggccttggc cgcctgggcc gccgcgctgc gcgatgaagg gatcgaggtc cagcagctgt
     8581 cggcggatcg gctggccgga ttggagccga agctggctgt tgctcagcaa tcaggacgca
     8641 gcgctgcacc ttgtttccat gtgcccggcg aagctcagct acgcaatcca cggcaccttc
     8701 aggcgctctc tctggcctgc cgcgccgccg gcgtcacgat tgcttccaac accgctgcct
     8761 ctggagcagt gatcacagcc ggtcaggtgg aggcgatcga aacgagcgcc ggtccgttac
     8821 gggcgggcca atactgtttc accgccggcg cttggacggg ccagcttctc agccaactgg
     8881 gcatttctgt cggcatcgtg ccgatccgcg gccagatggt cctgtttcgc tgcgccgagc
     8941 cgccggtgcg gcacatcatt aatgaaggct cccgctatct cgtgccgcgc gacgacggcc
     9001 gcctcttggc cggctccacg gaagaggaag tcggcttcga caaacggacg accgacgacg
     9061 cgattgccga gctgacgcgg ttcgccagaa gcctcattcc cgcattggcg ggcgccgaga
     9121 tcgagcagac ctgggccgga ctgcgtcccg gttcgttcga cgggctgccc tacctcggcc
     9181 ggctgccggg actgcccaac gccttcatcg cttccggcca ttttcgcagc ggcctgtctc
     9241 tctcgccggc aacggccgtc gtgatgagcc aattgatccg cgacgaacca acggaaatcg
     9301 atctcgcgcc gtttcgcgtg ggacggtgag tcacgcgtgc gtggttgctt tagccgtcga
     9361 gcgtgtcggg atagacgacg atctcgacgc ggcggttctt ggccttgccg acgggcgtgg
     9421 cgttgctggc cagtgggtga ttgtcgccca tggccaggat cgagagctgc cgggcgggaa
     9481 cgcggccgcg ctgcacgagc tgcgtgaaga ccgcctgggc ctgggccgtt gtgagcgagt
     9541 gagccgccgt cggctgcgag gccagcgcgt cgtcggtgtg ggcttcgacg acgatccgct
     9601 gccgcggata cgaccgctgc acggcggcag ccacctcgtc caggatgcgg tagccgtcgg
     9661 cctgaatctg cgaagcgccc ggcaggaaca acttgtcgcc tggaatctcg atgcggatca
     9721 ccgcgccgtc ctggcggatg tcgaggccgg gaatggcgat cgtcgccagc gactggcgga
     9781 tgctgctgtt ggccgtgatc gttgcgcctc ctcgggcccg agtcgaagcc tgcagcgcgg
     9841 tgacctgctt gtcggcctcc tgcttggcga gcagtgattc gcgggcctgc cgggcggcca
     9901 catcgatctg ctgctggagc agccgcacct ggttctcggc ggcttgcttg gcctgctcgt
     9961 gctgggcgag ctggcggtgc aggtcggcgt tgttcacatc gagctggctg acgcggcggt
    10021 tcaggtcttg cacctgcgcc gtcaatgact gagtctgctg ctggtaggcc gtggacgtcg
    10081 ccgcaggttg ggcggtcgcg gcagcaaatg gcccttgctg gcagccggcg cagacggcca
    10141 gcgccaaaac gtgcatcgcc agtaacagcc gaggagacat aacgaattct gcgctgcggt
    10201 tgacatcctg ccgcccgctg ccgccaggcg cggcggatgt taccgctgcc cgctggccct
    10261 agcaaccgca aatccgctag ctcttgccat cgctagctgg cagcgggcct agaatgccgg
    10321 catggcccgc cagcagtccg accgcgaaga cctgct
//
LOCUS       DASRJB010000704         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1893232, whole genome shotgun sequence.
ACCESSION   DASRJB010000704 DASRJB010000000
VERSION     DASRJB010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893232"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFB80_18710"
     CDS             complement(<1..560)
                     /locus_tag="VFB80_18710"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZN35872.1"
                     /translation="MTPDRAYAELLVHLRQMALVESTLEVLAWDELTLMPDAAIEHRG
                     SQLAHLAGWHHDLAASPRIDDWLAAVELSPLVADPHSAAAVNVREARRQHQRLSRLPR
                     SLVEELARVTTAAQHEWKLARAASDFKHFEPWLAAIITLKREQALCLAEAGPLYDPLL
                     AEYEAGLRTADVAAILEVLRSELTSLL"
     gene            657..2114
                     /locus_tag="VFB80_18715"
     CDS             657..2114
                     /locus_tag="VFB80_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35873.1"
                     /translation="MLTLAEEIGLGGYHLASRVRRAFHALPAAEIVQVMQEVREASVQ
                     RHLVYLREGQADIVGLFPLPLTALPDQISYLHFISLTIQNALKRLPELYFQDFALREA
                     LRVPPEEEAWLLECWGASQRESNPIFGRLDAVIDFTSPMWKNSLRFMEPNMSGIGGLY
                     LVPTAERLLEEIVLPRLAVHDEDLQLALAPDIRELLMQTIRDHLEAIGRPARTICFVE
                     AKYAGSGIDEQEDVARYFHDQYGLKVLHADPGELTILGDEVSYAGETVDLVYRDYLVS
                     DLIQLSKSGVNVQPMKTLFKQNRVISSIAAELDQKSCWEVLTDPKIAERHFSSDERQI
                     FRRHILWTRIVSDRKTTTPDGRERDLLEFVRRERETLVLKPNRAYGGEGVLIGVATTE
                     AQWDAALNTALRDPNRWVVQQLASIPVSEFPVVGPDGRVHSEPFHVVMGFAPTQYGVA
                     ILGRASQKQVVNVAQRGGMCVVFIGRHSGRLHGPG"
BASE COUNT          363 a          723 c          690 g          354 t
ORIGIN      
        1 agcagcgacg tcaactcgct tcggagcacc tccaggatcg ccgcgacatc tgccgttcgc
       61 aggccagcct catactcggc caacagcggg tcatagagcg gcccagcttc ggccaggcac
      121 aacgcctgct cgcgcttcag cgtgatgatg gccgccagcc agggctcaaa atgtttgaag
      181 tcgctcgccg cgcgggccaa cttccactcg tgctgtgccg ccgtcgtcac gcgggcgagt
      241 tcttccacca ggctccgagg caagcgcgac agccgctggt gttgacgccg ggcttcgcgc
      301 acgttgacgg ccgccgcact gtgcggatcg gccaccagcg gcgaaagctc gacggccgcc
      361 agccagtcgt cgattcgcgg cgaagcggcc aggtcgtgat gccagccggc cagatgggcg
      421 agctgagagc cgcggtgctc gatcgccgcg tcgggcatca gcgtcagctc gtcccaggcc
      481 agcacctcca gcgtcgattc gaccagggcc atttgcctca ggtgcacgag cagctcggca
      541 taggctcggt cgggcgtcat cgcagaatcg tagccgaatt cgttatagta gacatctcgc
      601 tccgcgagct gcccgcctca cgcggagcgt gaggactacg cttcaggaag ccgcccgtgc
      661 tcacgctggc cgaagaaatt ggactcggag gttatcacct cgccagccgc gttcgccggg
      721 cgtttcacgc gttgcccgcg gcggagatcg tgcaggtcat gcaggaggtc cgcgaggctt
      781 cagtccagcg gcacctggtc tatcttcgcg agggccaggc cgacatcgtg ggcctgttcc
      841 cgctgccgct cacggccctg cccgaccaga tttcctacct gcactttatc tcgctcacga
      901 ttcagaacgc cctcaagcgg ctgccggagc tgtacttcca ggatttcgcc ctgcgtgaag
      961 ccctccgcgt gccgccggag gaagaggcct ggctgctgga gtgctggggg gccagccagc
     1021 gggagagcaa cccgatcttc ggccggctcg acgcggtgat cgacttcacc agcccgatgt
     1081 ggaagaactc gctgcggttc atggagccca acatgagcgg catcggcggg ctgtacctcg
     1141 tgccgaccgc cgagcggctg ctggaggaaa tcgtgctgcc gcggctcgcc gtccacgacg
     1201 aggatttgca gctcgcgctg gccccggaca ttcgcgagct attgatgcag acgatccgcg
     1261 accacctgga ggcgatcggc cggccggccc gcacgatctg cttcgtcgag gccaagtacg
     1321 ctggcagcgg catcgacgag caggaagacg tggcccgcta tttccacgat cagtacggcc
     1381 tgaaggtcct gcatgccgat ccgggcgagc tgacgatcct gggcgacgaa gtctcctacg
     1441 ccggcgagac ggtcgatctg gtctatcgcg actacctggt cagcgacctg atccagctca
     1501 gcaagtccgg tgtcaacgtg cagccgatga agacgctgtt caagcagaac cgggtgattt
     1561 cgtcgatcgc ggccgagctc gaccagaaga gctgctggga ggtgctgacc gatcccaaaa
     1621 tcgccgagcg gcacttcagc tccgacgagc ggcagatttt ccgccggcac attctctgga
     1681 cgcggatcgt ctcggaccgc aagacgacca cgcccgacgg ccgcgagcgc gacctcctgg
     1741 aattcgtccg ccgcgagcgc gagacgctgg tcctcaagcc gaaccgggcc tacggcggcg
     1801 aaggcgtgct catcggcgtg gccaccacgg aggcgcagtg ggacgcggcg ctgaataccg
     1861 cgctccgcga tcccaaccgc tgggtcgtgc agcagctggc gagtattccc gtcagcgagt
     1921 tccccgtcgt cggccccgac ggccgcgtgc acagcgagcc attccacgtt gtgatgggct
     1981 tcgccccgac acaatacggc gtcgcgatcc tcggccgcgc ctcgcagaag caagtcgtca
     2041 acgtcgccca gcgcggcggc atgtgcgttg tcttcatcgg ccgccacagc ggccggttgc
     2101 atggaccagg gtgagtcgcc cgcttaattg
//
LOCUS       DASRJB010000705         5520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1893497, whole genome shotgun sequence.
ACCESSION   DASRJB010000705 DASRJB010000000
VERSION     DASRJB010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893497"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFB80_18720"
     CDS             complement(<1..1138)
                     /locus_tag="VFB80_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35874.1"
                     /translation="MSSRPPAPSPQPPAPPAGEQLKPFRIQLAERINRLPQYVLARVN
                     SLLHQKRQNGQDVIDLGMGNPSEPPHELVIEKLAEAARDPNNHGYSKANGILNLRREV
                     AGKYLKKYGVRLDPDSEVIVTIGSKEGFSHMCLAAMGAGETAIIPAPYFPAHMYAIVM
                     ASGNVITLDVSDSEQFLSNVALMCQHMTPRPKLLVVNYPHNPSSKTIEPEFFVEVVKL
                     ARRYGFMVISDFAYADVAFDGYQPPSFLAAPGAKDVGVEFTTMSKGYNMAGWRVGYCC
                     GNAEMVRALGIIKGYYDYGLFQAIQIAAIVALRHTDAAVEAQSRLYAARRDLLLDSLR
                     KQGWQVETPRAGMFVWAKYPEPLASTLSSLDFSMKLLEEANVAVS"
     gene            complement(1493..2164)
                     /locus_tag="VFB80_18725"
     CDS             complement(1493..2164)
                     /locus_tag="VFB80_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZN35875.1"
                     /translation="MPSLPAPKAVVFDLDGLMFNTEELYQLVGAELLRRRGCEFTQEL
                     LDQMMGRPSAVALQIMIDTHSLKASVQELLSETDEIFPGILRERLAPMPGLLELLAAL
                     ESRQIPKAIATSSRRSFVDRVLGQFGFLPRFSPILTSEDITHGKPHPEIYLLAARRLG
                     IETREMMVLEDSQNGCRAAIAAGAVTVAVPGSHSARHDFSGAAFIAASLADPQIYERL
                     GLTST"
     gene            2320..3711
                     /locus_tag="VFB80_18730"
     CDS             2320..3711
                     /locus_tag="VFB80_18730"
                     /inference="COORDINATES: protein motif:HMM:NF023990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HZN35876.1"
                     /translation="MNRAGEHEPESRSAAPLREHYIPLRPADLVEKLANEPAVTIFER
                     EQFRQLCQLLQATIHHDYRSRLEELKAAYAPFDPDDDAAEQFPLEKHELAARCRRLFD
                     RFDSLLMRANYRRLSREDLEQAIRAPNTAGPNLHLDLKLFERLEIYVRGRGEQTHSVR
                     SWRTLWRARAEQVPIHRRLAIIYRLAQRTPLTEPQGTREVVLKLFKNIPEQDVETLLP
                     GASIKIGWLEQAQIALPTLSGIGLTLFKLLKGAAAVAFAGIYGMLAFLGLIGGAIGYG
                     VRSFYSYLRTREKHQLSLTRHLYFQNLDNNAGVIYHLLAEAEEQEFREIVLAWWLLWR
                     GGLAGASAPQIDVAAEAWLRERCGIDADFEIGDALAKLARLGLAQVPASGRREPRDIA
                     AHLTARRSRETQGHRESDASRSPARWQAVSIEAALETLDRAWDEQFDYHRPRTQRIDA
                     TPQAHPRIWRRAA"
     gene            complement(3668..4330)
                     /locus_tag="VFB80_18735"
     CDS             complement(3668..4330)
                     /locus_tag="VFB80_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35877.1"
                     /translation="MSRLDQEIERLLAMLAQAGVRPTWHWIALVEAADVQYAGSAGQT
                     LRIPRQALRRQGVYSSRFDEHLRAGYSWINFSAAGIAEGALVVVVEIPRTASGAPIDR
                     VSVNLSGSARNRVELFDARLDAAAVDGIVRACAELRAGKIDPTALGQRLVQLTSAPGT
                     PAWVYRDAAEWRRQLEAAWQWAQSGDERGLGRAEGIAIALRGWAESLTPPAAKSADEP
                     AE"
     gene            complement(4443..>5520)
                     /locus_tag="VFB80_18740"
     CDS             complement(4443..>5520)
                     /locus_tag="VFB80_18740"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN35878.1"
                     /translation="RGGSWKDPANFLTSDSRLEAPAGASDDAFGLRCVLALVQDAPES
                     RPAEFRPTAQEKFVPASSKLEMLWNEGEFTEGPALAPDGSIFFSDIGNAIYRYDPKAG
                     KTAVFRQPSGRSNGLKFDQQGRLIACEGANTGGNRRISITTGIDGAKDGQVKTLTERV
                     DGKRFNSPNDLAIDGQGRVYFTDPRYVGDEPRELDFEAVFLVSTGGEAKVATREVSKP
                     NGVLVSPDGKTVYVSDNNPDGARQLVAFEVQQPGGGLATKRLLWDFGAGRGIDGMTLD
                     TAGNIYATAGTGDKAGVYVFAPDGKNLALVPTPGDPTNCCFGGWSDSGMLYVTCANSK
                     SAGTKYGLYRIKLNATGYAVVKPK"
BASE COUNT          932 a         1790 c         1801 g          997 t
ORIGIN      
        1 ggctgacggc gacgttggct tcttccaaga gtttcatcga gaaatctagc gatgacagcg
       61 tgctggcgag gggctcggga tacttggccc agacgaacat gccggcccgc ggcgtttcga
      121 cttgccagcc ctgcttgcgc agcgaatcca gtagcaaatc gcggcgggcg gcgtagagcc
      181 gcgattgcgc ctcgacagca gcgtcggtgt gccgcaaggc gacgatggca gcgatctgga
      241 ttgcctgaaa caggccgtag tcgtagtagc ccttgatgat gcccagggcc cgcaccatct
      301 cggcgttgcc gcagcaatag ccgacgcgcc agccggccat gttgtagccc ttgctcatcg
      361 tggtgaactc gacgcccacg tctttggcgc ccggtgcggc caggaagctc ggcggctgat
      421 agccgtcgaa ggctacgtcg gcataggcaa aatcgctgat gaccatgaag ccatagcggc
      481 gggcgagctt cacgacctcg acaaagaact ccggctcgat ggtcttgctc gacggattgt
      541 ggggataatt gacgaccaac agcttgggcc gcggcgtcat gtgctggcac atcagggcga
      601 cgttcgacag gaattgctcg ctgtcggaaa cgtcgagcgt gatcacgttg ccggacgcca
      661 tcacgatcgc gtacatgtgg gcggggaaat acggcgcggg gatgatcgcc gtctcgccgg
      721 cccccatcgc cgccaggcac atgtggctga acccttcctt ggagccgatc gtgacgatca
      781 cttcgctgtc ggggtccagc cgcacaccgt atttcttcaa gtatttgccg gcgacctcgc
      841 ggcggaggtt caagatgccg ttggctttgc tgtagccgtg gttgttgggg tcgcgggccg
      901 cttcagccag cttctcgatc accagctcgt ggggcggctc gctgggattg cccatcccca
      961 ggtcgatcac atcctgccca ttctgccgct tctggtgcag caggctattt acccttgcca
     1021 gcacatactg cggcagccga ttgatccgct ccgccaactg aatgcggaac ggcttgagct
     1081 gctcgccggc gggcggggcg gggggctggg ggctgggggc tgggggacgc gaggacatcg
     1141 ttgggcgatc ttcaaaactg ggcagtcatt tccctttcaa catactcatc gggtcgaatc
     1201 ggcagttggg tcgaatggac agaccccaaa ggcgggtggt tcgccggatg cttattctgc
     1261 atatgcgggt aaattgtttt cggtgccgtc tgctcctcaa gtctttctgc aataacagtt
     1321 tacgtccaaa tcatgctgca tatccttatg cagattatgc acgctaagga attctatgca
     1381 cgccaatgcg tggcgaagga ttcctgccgc cgcggctcgc cagcgttcgt ccggcatcca
     1441 ttgtacactt ttgcaaaggc gatacaagct gcacggcaaa tcggccatgg cgtcaggtcg
     1501 aggtcagacc gaggcgttca tagatttgcg ggtccgccaa ggaagcagcg atgaaggccg
     1561 ccccgctgaa gtcgtgccgg gcgctgtgcg agccggggac ggcgacggtg accgcgccgg
     1621 cggcaatagc ggcccggcag ccgttctggc tgtcttccag gaccatcatt tcgcgggtct
     1681 cgatgcccag ccggcgggcg gccagcaggt agatttccgg gtggggcttg ccgtgcgtga
     1741 tgtcctcgga cgtcaggatc ggcgaaaacc gcggcaaaaa gccgaattgg cccagcacgc
     1801 gatccacgaa cgaccgccgg ctgctggtgg cgatggcctt gggaatctgc cgcgactcaa
     1861 gtgccgccag cagttcgagc aagcccggca tcggcgcgag tcgctcgcgg agaattcccg
     1921 gaaagatctc gtcggtttcg gatagcagct cttgcacgct cgccttcaga gagtgcgtgt
     1981 cgatcatgat ctgcagcgcg accgcgctgg gccggcccat catctgatcg agcagctcct
     2041 gcgtgaactc gcagccgcgg cggcggagca attcggcccc cacgagctga tagagctctt
     2101 cggtgttgaa catcaggccg tcgaggtcga agacgacggc cttgggtgct ggcagtgatg
     2161 gcatgggatt caatgtttag tgtttggtgt tttgtgttcg gggagaagcg ccaaccgata
     2221 tcaccagata ccaaacccga tacacctaac accaaacact gcgtcatttt gccttgaggc
     2281 gcgattttgc gcaaggccgg caagcgctag cagaccagca tgaacagggc aggggagcac
     2341 gagccggaat cgcggtctgc cgcgccgctg cgtgagcact atatcccctt gcgccccgcg
     2401 gacctggtcg agaagctggc caacgagccg gcagtgacga tctttgagcg cgagcagttc
     2461 cggcagctct gccagctgct gcaagcgacg atccaccacg actaccgctc gcggctggag
     2521 gagctgaagg cggcctacgc cccgttcgat cccgacgacg acgcggccga gcagttcccc
     2581 ctggagaagc acgagctggc cgcccgctgc cggcggctct tcgaccgctt cgattcgctc
     2641 ttgatgcggg ccaactaccg ccgcctgtcg cgcgaggacc tggagcaggc catccgcgcg
     2701 ccgaataccg ccgggccgaa cctgcacctc gatttgaagc tcttcgagcg gctggagatt
     2761 tacgtccgcg gccgcggcga gcagacccat tcagtgcgct cgtggcgcac gttgtggcgg
     2821 gcccgcgccg agcaagtccc aatccaccgc cggctggcga tcatctatcg gctggcgcag
     2881 cgaacgccgc tgaccgagcc gcagggcacg cgcgaagtcg tactcaagct gttcaagaac
     2941 attccggagc aggacgtcga gacgctgctg cccggagcga gcatcaagat cggctggctg
     3001 gagcaggcgc agatcgcgct gccgacgctg tcggggatcg gcctgacgct gttcaagctg
     3061 ctcaagggcg cggcggcggt cgcgtttgcc ggcatctatg gcatgctcgc cttcctggga
     3121 ctgatcggcg gggcgatcgg ctacggcgtg cggtcgttct acagctacct gcggacgcgc
     3181 gagaagcacc agctcagcct gacgcggcat ctgtatttcc agaatctcga caacaacgcg
     3241 ggcgtgatct atcacctgct cgccgaggcc gaggagcagg agttccgcga gatcgtgctc
     3301 gcctggtggc tgctctggcg cggcggcctg gcgggcgcaa gcgcgccgca aatcgacgtg
     3361 gctgccgaag catggctgcg cgagcggtgc ggcatcgacg ccgatttcga gatcggcgac
     3421 gcgctggcca agctcgcccg gctggggctg gcccaagttc cggcgagcgg gaggcgtgag
     3481 cctcgcgata ttgccgcgca cctcaccgct cgacgctcgc gggagactca gggccatcgg
     3541 gagtctgacg cctcccgctc gcctgcccgt tggcaggccg tttcgatcga agccgccctc
     3601 gaaacgctcg accgcgcctg ggacgaacag ttcgattacc accggccgcg cacgcagcgt
     3661 atcgacgcta ctccgcaggc tcatccgcgg atttggcggc gggcggcgtg aggctctctg
     3721 cccatccccg cagggcgatc gcaatgccct cggcccggcc caggccgcgc tcgtcgccgc
     3781 tttgggccca ttgccaggcc gcttccagct gccggcgcca ctcggctgcg tcgcgataaa
     3841 cccaggccgg cgtcccgggc gcggaagtca actgcaccag ccgctgaccg agcgctgttg
     3901 gatcgatctt gccggcccgc aattcggcgc acgcccgcac gatgccgtct accgcggcag
     3961 catccagcct ggcatcgaac aactcgacgc gatttcgcgc tgagcctgag aggttgaccg
     4021 acacccgatc gatcggtgca ccgctggccg tgcgcggaat ttcgaccacc acgaccaatg
     4081 ctccctcggc gatgcctgcg gcggaaaagt tgatccacga atagccggcc cgcaagtgtt
     4141 cgtcgaaccg cgaggaatag acgccctgcc ggcgcagcgc ctgccgggga atccgcaggg
     4201 tttgcccggc gctgcctgcg tactgcacgt cggcggcttc taccagcgcg atccagtgcc
     4261 aggtcgggcg cacgcccgct tgcgcgagca tcgccagcag ccgctcgatt tcctgatcca
     4321 gccggctcat attccagcat ggcgaacagt agggtgggac aaggccgctg cggccgccgg
     4381 caccgtttct gagctggccc gcggcccggt gggcctgcgc tcgcggactc gctagtccca
     4441 ccttacttcg gcttcaccac ggcatagccc gtcgcgttga gcttgatccg gtagaggccg
     4501 tacttcgtgc cggcggactt gctgttggca caggtgacgt acagcatccc cgaatcggac
     4561 cagccgccaa agcagcagtt ggtggggtcg ccgggcgtcg ggacgagggc caggttcttg
     4621 ccgtccgggg caaagacgta gacgcccgct ttgtcgcccg tgccggcggt cgcatagatg
     4681 ttgcccgccg tgtcgagcgt catgccgtcg atgccccggc ctgcgccgaa atcccacagc
     4741 agccgcttgg tggccagtcc gccgcccggc tgctgcacct cgaacgccac cagctgccgc
     4801 gcgccgtcgg gattgttgtc cgagacatag accgtcttgc cgtccggtga aacgagaacc
     4861 ccgttgggct tgctgacctc gcgtgtcgcg accttcgcct cgccgccggt agaaacgaga
     4921 aacaccgcct cgaaatcgag ctcccggggc tcatcgccga catagcgcgg atcggtgaaa
     4981 tagacgcgcc cctggccgtc gatcgccagg tcattggggc tgttgaaccg cttgccgtcc
     5041 acccgctcgg tcagcgtctt gacctggccg tctttcgcgc cgtcgatgcc ggtggtgatc
     5101 gaaatccgcc ggttgccgcc ggtgttggcc ccttcgcagg cgatcagccg gccctgctgg
     5161 tcgaatttca ggccgttgct gcggccgctc ggctggcgga agacggcggt cttgcccgcc
     5221 ttgggatcgt agcgatagat ggcgttgccg atgtcggaga agaagatcga gccatcgggc
     5281 gccagggccg gcccttcggt gaactcgccc tcgttccaga gcatttccag cttggagctg
     5341 gccggaacga atttttcttg ggccgttggc cgaaactcgg ccggcctgct ctccggcgca
     5401 tcttgaacca aagcgagcac gcagcgcagg ccgaacgcgt catcgctcgc cccggcaggc
     5461 gcttcgagcc gcgaatcgct ggtcaggaaa ttcgccgggt ccttccagct cccgccgcgc
//
LOCUS       DASRJB010000706         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2462770, whole genome shotgun sequence.
ACCESSION   DASRJB010000706 DASRJB010000000
VERSION     DASRJB010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462770"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2130
                     /locus_tag="VFB80_18745"
     CDS             <1..2130
                     /locus_tag="VFB80_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HZN35879.1"
                     /translation="CPDFRSNTLGTCKHILHVQGRAKRRFDAADLKKPYRRKRIAVHL
                     NYDRDVTLRLAAPERMKGEVADVVKPLLDKPIDNVQLLLKRLKKLDQLGQEFHVYPDA
                     EEFIQQRLVRDRLQEQMAEIRRGPAKHPLRTSLLKVPLLPYQLDGVAFAASAGRVILA
                     DDMGLGKTIQGVGLAELLAREAGIRKVLIVCPATVKAQWKSEIHRFCDRDVQLVAGAS
                     AHRREQYARDCFFTVCNYEQVLRDILDIEQVKWDLIILDEGQRIKNWESKTARVIKGL
                     RSPFALVLSGTPLENRLEDLYSVVQFIDQRRLGPGFRFFNQHRIVDEKGKVLGYKNLE
                     ALRENLRPVLLRRTRDSVKLELPPRSTEIVRITPTDEQLELHRAHMQIVAATVRKRFL
                     TEMDLLRLRIALLMCRMSANSTFLVNKEPPGHSSKLDHLAELFDELFDEAGRKVVLFS
                     EWATMLDLIQPLLAKRRLTFVRLDGSVPQKKRPELVHEFQNNADCKLFLTTNAGSTGL
                     NLQAANTVINVDLPWNPAVLEQRIGRAHRMGQTQPVQVYVLVTEGTLEENLLTTLSAK
                     KELALAALDPDADADTVEMVSGMDELKGRLEILLGAKPIAPLDETAKQQAEGDSREAS
                     ERRERVSAAGGELLGAAFKFLGELVASQSPQVAQPPLAAELRNRLAECVQEDEGGKQR
                     LTVTLPDKAALHSLADALAQLLAVDKP"
     gene            complement(2196..>2937)
                     /locus_tag="VFB80_18750"
     CDS             complement(2196..>2937)
                     /locus_tag="VFB80_18750"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35880.1"
                     /translation="AGANVTCAEVWLTKLETYLEAHKLDDVRYWLEKTRLTLSVASFQ
                     GGLLASQGEARREAWELFRGRLDLCRELSIGTIVVACDVPGPLSQQTIERVQASLVQV
                     AQEAGQRGLKAALEFQANSSFGNNLQTAAALVADVGSPHLGLCLDAFHWHVGPSKTED
                     LGYLTAANLFHVQLCDLADTPRELAKDSHRILPGEGDIHLAPLLERLRQIDYRGCVSI
                     ELMNPQLWQVPALQLADAAMASLRRLLP"
BASE COUNT          542 a          978 c          958 g          459 t
ORIGIN      
        1 tgtcccgact ttcgcagcaa cacactgggc acctgcaagc acatcctcca cgtgcagggc
       61 cgggcaaaac gccggttcga cgcggccgac ttgaagaagc cctaccgccg caagcgaatc
      121 gccgtccacc tgaactacga ccgggacgtg actttgcggc tggccgcgcc ggagcgaatg
      181 aagggcgaag tcgccgacgt cgtcaagccg ctgctggata agccgatcga caacgtgcag
      241 ctgctgctga agcggctgaa gaagctggat cagctcggcc aggagtttca cgtctatccc
      301 gacgccgagg agttcatcca gcagcggctg gtccgcgacc ggttgcagga gcaaatggcc
      361 gaaatccgcc ggggccccgc caaacacccg ctccgcacat cgctcttgaa agtgccgctc
      421 ttgccgtatc agctcgatgg agtcgccttc gccgcatcag ccgggcgggt gatcctggcc
      481 gacgatatgg gcctgggcaa gacgatccaa ggcgtcggcc tggccgagct gctggcccgc
      541 gaagccggca tccgcaaagt gctgatcgtc tgccccgcca cggtcaaggc acagtggaag
      601 agcgaaatcc accgcttctg cgatcgcgac gtgcagctcg tcgcgggcgc gagcgcccac
      661 cgccgcgagc aatatgcccg cgactgcttc ttcacggtct gcaattacga gcaggtgctc
      721 cgcgacatcc tcgacatcga gcaggtgaag tgggacctga tcatcctgga cgaggggcag
      781 cggatcaaaa actgggagtc gaagacggct cgcgtgatca agggcctccg ctcgccgttc
      841 gcgctcgtgc tctcgggcac gccgctggag aaccgcctgg aagacctcta ctccgtcgtg
      901 cagttcatcg accagcggcg gctcggcccc ggcttccgct tcttcaacca gcaccgcatc
      961 gtcgatgaga agggcaaggt gctgggctac aagaacctgg aagccctgcg cgagaacctg
     1021 cggccggtcc tgctgcggcg gacgcgcgac tcggtgaagc tggagctgcc gccgcgctcg
     1081 accgagatcg tccgcatcac gccgaccgac gagcagctcg agctgcaccg cgcgcatatg
     1141 caaatcgtcg cggccaccgt ccgcaagcgg ttcctcaccg agatggatct cttgcggctg
     1201 cggatcgccc tcctgatgtg ccgcatgtcg gccaatagca cgttcctggt caacaaggag
     1261 ccgccgggac attccagcaa gctcgaccac ctggccgagt tgttcgacga gctgttcgac
     1321 gaagcaggcc gcaaggtcgt cctcttctcg gagtgggcga cgatgctcga cctgatccag
     1381 ccgctgctgg ccaaacggag gctaacgttc gtgcggctgg acggctcggt gccgcagaag
     1441 aagcggccgg agctcgtcca cgaattccag aacaacgccg attgcaagct gttcctcacg
     1501 accaacgccg gctccacggg gctgaacctg caggctgcga acacggtgat caacgtcgat
     1561 ctgccctgga acccggcggt gctggaacag cggatcggcc gggcccaccg catgggccag
     1621 acgcagccgg tgcaggtcta tgtgctggtg accgagggca cgctcgagga gaacctgctc
     1681 acgacgctct ctgccaagaa agaactagcg ctggccgcgc tcgatcccga cgccgatgcc
     1741 gacacggtcg agatggtcag cggcatggac gagctgaagg gccgactgga aattctgctc
     1801 ggcgccaagc cgattgcccc gctcgacgaa acggcgaagc aacaggccga gggcgattcg
     1861 cgcgaagcga gtgagcgccg cgaacgggtc tcggccgccg gcggcgaatt gctcggcgcg
     1921 gccttcaagt tcctcggcga gctggtcgcc agccaatcgc cgcaggtcgc ccagccgccg
     1981 ctcgccgcag aacttcgcaa ccgcctggcc gagtgcgtgc aggaagacga gggtggcaag
     2041 caacgcctca ccgtgacgct gcccgacaaa gccgcgctgc acagcctcgc cgacgccctg
     2101 gcgcagttgc tcgcagtgga caagccgtag cataagcgtc ctcgcttgtg catcggtcaa
     2161 accgccggag ccatgagcaa gcgaggacgc ttgctctacg gtagcagtcg ccgcagcgaa
     2221 gccatcgcgg cgtcggcgag ttgcagggcg ggaacctgcc agagctgcgg gttcatcagc
     2281 tcgatcgaaa cgcagccgcg gtagtcgatt tgccggaggc gttcgagcag cggggcgagg
     2341 tggatgtcgc cctcgccggg aagaatgcga tggctgtcct tggccagctc gcggggcgtg
     2401 tcggccaggt cgcagagctg gacgtgaaac aggttcgcgg cagtgaggta gccgaggtcc
     2461 tcggtcttgc tcggcccgac gtgccagtga aaggcgtcga ggcagaggcc caagtgcggg
     2521 ctgccgacat cggccaccag ggcggcagcc gtttgcaggt tgttgccgaa gctgctattg
     2581 gcttgaaatt cgagcgccgc tttgaggccg cgctgtcccg cttcctgcgc gacttgaacg
     2641 agtgaagctt gcacccgctc gatcgtctgc tgcgagagcg ggcccggcac gtcgcaggcc
     2701 accacgattg tgccgatcga cagctcgcgg cacagatcga gccggccgcg gaacagctcc
     2761 cacgcctcgc gcctcgcctc gccctggctc gccaaaaggc cgccctgaaa gctggccacg
     2821 ctcagcgtca gccgcgtctt ctcgagccag taccggacgt cgtcgagctt gtgcgcttcg
     2881 aggtacgtct ccagcttcgt cagccagacc tccgcgcagg tcacgtttgc cccggca
//
LOCUS       DASRJB010000707         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1055393, whole genome shotgun sequence.
ACCESSION   DASRJB010000707 DASRJB010000000
VERSION     DASRJB010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055393"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2070
                     /gene="thrS"
                     /locus_tag="VFB80_18755"
     CDS             <1..>2070
                     /gene="thrS"
                     /locus_tag="VFB80_18755"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZN35881.1"
                     /translation="GKVVGLDYQLPADGEARLRIFTMKDPEALGVMRHSAAHVMARAV
                     MRLKKGVQLAFGPTVEGGFYYDIQSPDPVREEDFPAIEAEMQKIIDLGEPFERIETPR
                     AKALEICAGLEQKYKVEHINTGLADHASMSFYRQGEFLDLCRGPHVPSPKVIGAFKLL
                     SIAGAYWKGDANNAQLQRLYATAFFTPQELEEHLKKIDEAKRRDHRVLGKQLQLFTIS
                     NDVGPGLCLWLPKGAMIRSQLEDFIKQELARRDYLPVYTPHIGRIQMYQTSGHFPYYM
                     ESQFPPFYLDPFMQSVQQLFYLLFQSKVSDERFVGMFEEAVQKNDNTLVPNSFPGFGG
                     ASGNTAKTASEKKKAITDWFNEQEAYLLKPMNCPHHIQIYQAERRSYRELPLRLAEFG
                     SVYRFEKTGQLNGMTRVRGFTQDDAHLFCMEDQVPAEFSGCIEMTQFVLTCLGLENYR
                     VRLGFRDPASDKYVGGAEVWERAQNSLEDVCSRLKIDYTPEPGEAAFYGPKADFVVTD
                     CIGREWQLGTVQLDYNLPSKDRFGLEYIGADNQPHQPAMIHRAPLGSLERFVGVLIEH
                     FAGAFPLWLAAEQVRVLTVSEKSDAYGREVEKQLMDAGLRVSGDYRGAKLNAKVRDAQ
                     VQLIPYMFVVGERDREAGTVAVRDRLEGDLGSMPLESAIAKLRDEIAAKTVRQVAARQ
                     AVAITDQ"
BASE COUNT          408 a          678 c          640 g          344 t
ORIGIN      
        1 atggcaaggt cgtcggcctc gattaccagc tgccggcgga cggcgaagcc aggctccgca
       61 tcttcacgat gaaagatccc gaggcgctcg gcgtgatgcg gcactccgca gcccacgtca
      121 tggcccgggc ggtgatgcgg ctgaagaaag gcgtgcagct cgcgttcggg ccgaccgtgg
      181 aaggcggctt ctattacgac atccagtcgc ccgatccggt ccgcgaggaa gatttcccgg
      241 ccatcgaagc cgagatgcag aagataatcg acttgggcga gccctttgag cggatcgaga
      301 cgccgcgggc gaaagccttg gagatttgcg cggggctcga gcagaagtac aaggtcgagc
      361 acatcaacac gggcctggcc gaccacgcct cgatgtcgtt ctaccgccag ggcgagtttc
      421 tagacctctg ccgcggtccg cacgtgccca gcccgaaagt gatcggggca ttcaagctcc
      481 tctcgatcgc cggagcgtat tggaagggcg acgccaacaa cgcccagctg cagcggctct
      541 atgccaccgc gttcttcacg ccgcaagagc tggaggagca cctcaagaag atcgacgagg
      601 ccaagcgccg cgaccaccgg gtgctgggca agcagctgca gctcttcacg atcagcaacg
      661 acgtcggccc ggggctgtgc ttgtggctgc ccaagggagc gatgatccgc tcgcagctcg
      721 aggacttcat caagcaggag ctggcccgcc gcgactacct gccggtctat acgccgcaca
      781 tcggccgcat ccagatgtac cagaccagcg gccactttcc gtattacatg gagagccagt
      841 tcccgccgtt ctatctcgat ccgttcatgc agagcgtgca gcagctcttc tacttgctgt
      901 tccagtcgaa ggtcagcgac gagcgctttg tcggcatgtt cgaggaggcg gtccagaaga
      961 acgacaacac gctcgtcccc aattcgttcc ccggcttcgg cggtgcttct ggaaatacag
     1021 cgaagaccgc atcggagaag aagaaggcca tcaccgactg gttcaacgag caggaggcct
     1081 acctgctcaa gccgatgaac tgcccgcacc acattcagat ctaccaggcc gaacgccgca
     1141 gctaccgcga gctgcccctg cggctggccg agttcggctc ggtctatcgg tttgaaaaaa
     1201 ccggccagct caacggcatg acccgcgtcc gcggcttcac tcaggacgac gcccacctct
     1261 tctgcatgga agaccaggtc cccgccgagt tcagcggctg catcgagatg acgcagttcg
     1321 tgctgacttg cctgggcctg gagaactacc gcgtgcggct ggggttccgc gacccggcga
     1381 gcgacaagta tgtcggcggc gccgaagtct gggagcgggc ccaaaactcg ctcgaagacg
     1441 tctgctcccg gctgaagatc gactacacgc cagagccggg cgaagcggcc ttctacggcc
     1501 ccaaagccga ctttgtagtc accgattgca tcggccgcga gtggcagctc ggcacggtgc
     1561 agctcgacta caacctgccg agcaaggatc gtttcggcct ggaatacatc ggcgccgaca
     1621 accagccgca ccagcccgcc atgatccacc gcgccccgct cggctcgctg gagcggttcg
     1681 tcggcgtgct gatcgagcat ttcgccggtg cgttcccgct gtggctcgcg gccgagcaag
     1741 tccgcgtact cacggtgagc gagaagtccg acgcctacgg ccgcgaggtc gaaaagcagc
     1801 ttatggacgc cggtctccgt gtcagcggcg attaccgcgg cgcgaagctc aacgccaaag
     1861 tccgcgacgc ccaggtgcag ctcataccct atatgttcgt cgtcggcgag cgggaccgcg
     1921 aagctggcac ggtcgccgtc cgcgaccgcc tggaaggcga cctcggttct atgccattgg
     1981 aatccgctat cgccaaactg cgtgacgaaa tcgcggcgaa aacggtccgt caggttgcgg
     2041 cccgtcaggc cgtcgcaatt accgaccaag
//
LOCUS       DASRJB010000708         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1893802, whole genome shotgun sequence.
ACCESSION   DASRJB010000708 DASRJB010000000
VERSION     DASRJB010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893802"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..924
                     /locus_tag="VFB80_18760"
     CDS             103..924
                     /locus_tag="VFB80_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35882.1"
                     /translation="MLACRKFILLAALLPLSPGFASAQDDEQEAEATKRYQAHAQATA
                     ALYELKTAGEEPRQLKLRPESLLRWTNPLGGNKAHGELFLWTDRGRPAAVLSMYEWTD
                     VKGIVHEHHEWCSLSLDRIAAAGPKVWEPKSAGIELKPLPAAPPPADTPVRRLRQMRD
                     LGAGFAAEKTTREEEKRELRLLSQPAFRYEAGDPDVLDGAVFALVEATDPEAFLLLEA
                     RVADGKAAWHYGLARMNSLPLAASYQKQPVWEVPKVVGSQVYNRQDLPYTAFTIK"
     gene            complement(930..1256)
                     /locus_tag="VFB80_18765"
     CDS             complement(930..1256)
                     /locus_tag="VFB80_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN35883.1"
                     /translation="MSYFLTRPECSHHEIFSGVHIFTAAGDRMMISVVELAPRSEVKP
                     HSHPHEQMGLLLEGELTFTIGGQTQTLKPGQMWRIPGGVVHGCVAGDQPAKAIDVFHP
                     VREDYR"
     gene            complement(1285..2079)
                     /locus_tag="VFB80_18770"
     CDS             complement(1285..2079)
                     /locus_tag="VFB80_18770"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZN35884.1"
                     /translation="MRLTREIRFSLAPLPAGGEDPAAQNGWGGWWTDRLLAPHLRFQV
                     TVTGRPDADTGMLCNVKRLDAVFQQQCIPKLRTALAAGGLTAEAALLLAWEALARINL
                     TPCRPAALRLHVTPTLSLSISAEEENVMRVTQQFEFSAAHRLHSSKLSSEQNLEVFGK
                     CNNPHGHGHNYVVEVTVEGSPEPVSGEVVSILQLQRTVKSRVIQPLDHKNLDVEIEFF
                     RGVCSTVENIATYIFGQLDGQFAPAQLEAIRVYETPKTWAEVRRSD"
     gene            2155..>2446
                     /locus_tag="VFB80_18775"
     CDS             2155..>2446
                     /locus_tag="VFB80_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN35885.1"
                     /translation="MSSAAKVCVVTGGSSGIGAAIGERFFAAGYHVVSCSRRSSSAEL
                     PQEGRRRLSRAVDVADPQALTSFVQDTAAEFGRIDVLVNNAGNAPRRPVAELP"
BASE COUNT          394 a          779 c          848 g          425 t
ORIGIN      
        1 cgtcgccact ggctccgggt taaacgcagg acgccgcttg tgccggttag gacgggagcc
       61 tatgcttggg cttaagacat tctgtcttaa ctggaggctg ccatgctcgc gtgccgcaag
      121 ttcatccttc tcgccgcact cctgccgctc agccccggct tcgcctcggc tcaagacgat
      181 gagcaggaag cggaggccac gaagcgctat caagcccatg cccaggcgac ggcggccctg
      241 tacgagctca agaccgcggg cgaggagccg cggcagctca agcttcgccc cgaatcgctg
      301 ctccgctgga ccaatccgct cggcggcaac aaggcccacg gcgaattgtt tctgtggacc
      361 gaccgcggcc ggcccgcggc cgtgctctcg atgtatgaat ggaccgacgt caagggcatc
      421 gtgcacgagc atcacgagtg gtgctcgctg tcgctggacc gcatcgccgc tgccgggcca
      481 aaggtctggg agccaaaatc cgccgggatc gagctgaagc cgttgcccgc tgccccgccg
      541 ccggccgata cgcccgtgcg ccgcttgcgg caaatgcgcg acctgggcgc gggcttcgcc
      601 gccgagaaga ccacgcggga ggaagagaag cgcgagctgc ggctgctgtc tcagcccgcg
      661 ttccgttacg aagcgggcga tcccgacgtg ctcgacggcg cggtcttcgc gctggtcgaa
      721 gcgaccgatc ccgaggcctt cctgctgctc gaagcccgcg tggccgacgg caaggcggcc
      781 tggcactacg ggctggcccg catgaacagc ctccccctgg cggcttcgta tcaaaagcag
      841 ccggtgtggg aggtcccgaa agtggtcggt tcgcaggtgt acaatcgcca ggacttgccc
      901 tacacggcgt tcacgatcaa gtgatcgcct tagcgatagt cctcccgcac cgggtgaaac
      961 acgtcgatcg ccttggcggg ctggtctccc gcgacgcagc cgtgcacgac gccgccggga
     1021 atccgccaca tctggcccgg tttcagcgtc tgcgtctggc cgccgatcgt gaatgtcaat
     1081 tcgccttcca gcaagaggcc catctgctcg tgcggatggc tgtgcggctt gacctccgag
     1141 cgcggcgcga gctccacgac cgagatcatc atccggtcgc cggcggctgt gaagatgtgc
     1201 acgccggaga aaatttcgtg gtgggagcac tcggggcggg tgagaaagta ggacatgatt
     1261 tccgattgca gattgttaga ggcttcagtc gctgcggcgg acttcggccc aggtcttggg
     1321 cgtttcgtag acgcgaatcg cttcgagctg ggcgggggcg aattggccgt ccagctggcc
     1381 gaagatgtag gtggcgatgt tctcgaccgt cgagcacacg ccgcggaaga actcgatctc
     1441 cacgtcgagg ttcttgtgat ccagcggctg gatcacgcgg ctcttgaccg tccgctgcag
     1501 ttgcaggatc gagacgactt cgccggaaac cggctcgggg ctgccttcga cggtgacctc
     1561 cacgacgtag ttgtgcccgt ggccgtgcgg gttgttgcac ttgccaaaga cctcgaggtt
     1621 ttgctcgctg ctgagcttgg agctgtgcag ccggtgggcg gccgagaact cgaactgctg
     1681 ggtgacgcgc atgacgtttt cctcctcggc cgagatggat agcgagagcg tgggcgtaac
     1741 gtgcagccgc agggccgcgg ggcggcaggg cgtgagattg atccgggcga gcgcttccca
     1801 ggccagcagc agggcggcct cggcggtcaa gccgccggcg gccagcgccg tgcgcagctt
     1861 gggaatgcac tgctgctgaa agacggcgtc gagccgcttg acgttgcaga gcatcccggt
     1921 gtcggcgtcg ggccggccgg tcacagtgac ctgaaaccgc aagtgcggcg cgagcagccg
     1981 gtcggtccac cagccgcccc agccgttttg cgcggcgggg tcctcgccgc cggcaggcag
     2041 cggagcgagg ctgaagcgga tttcgcgggt gagccgcatc gcttgacgct gaacgggaat
     2101 ggggcctaca acggggcagc ccctctcagt tgatcttacg gctggcttcc aagcatgtca
     2161 tccgccgcga aagtctgcgt tgtcaccggc ggctcgtcgg ggatcggcgc ggcgatcggc
     2221 gagcggtttt tcgccgcggg ttaccacgtc gtcagctgct cgcggcgctc gtcgtcggct
     2281 gaattgccac aggagggccg acggcgactt tcgcgggcgg tcgatgtggc agatccacag
     2341 gcattgactt cgttcgtgca agacaccgcg gccgaattcg gccggatcga cgtgctggtg
     2401 aacaacgccg gcaacgcgcc gcggcggccg gtcgccgagc tgccgc
//
LOCUS       DASRJB010000709         5103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3029193, whole genome shotgun sequence.
ACCESSION   DASRJB010000709 DASRJB010000000
VERSION     DASRJB010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029193"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /locus_tag="VFB80_18780"
     CDS             complement(<1..2174)
                     /locus_tag="VFB80_18780"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN35886.1"
                     /translation="MLLLLGALPANAQEEADEENPYRPGLVATYSAAGLAATRLDETV
                     AFDWQDAAPDPRLPAGEFSAIWRGRLWAQADGKYQLLCFAQGSVEVKLAGKTVLTGQA
                     ESPQWLSSQPLELTFDRHELEITYRRSGPRGQLALFWTGPEFRLEPIAARFLAHNKER
                     SPSSEFERGRQLAAALRCAACHRDQSASVTPAPALDRLSGNVHEAWLVDWLSNHAAGS
                     SPRRMPDLGLTRDEAAAVAAWLLREPATKVEVKLPADRPAKAKSKNGKKKGEEEPPKP
                     TAGEGQRLLLTRGCLACHQLGELGESGLFGGGDLTKLAEKRPADFLIHWLSDPAAINR
                     SHRMPQFTFTPDELKSLDLWWQEQGAKPAAGSAGRAGEAERLVTSLRCANCHVLKRND
                     AGLAAASLAPLTAKSNWSSSCATNADRAKGRPGYRLADEDRAALVVYYSQPRPGTKLS
                     PQARGRDLLVQLNCLACHQREGIERNVAGTLRVPSRANEPISKNDGTGSVPAALQDRL
                     VAVAENHAALAPLVPAMTPPALNGVGDKLLDAALADSIRRQGPPHRPYLLVQMPKFAL
                     SDAQLADLTAYFTATDRVPESSPHAPREASVSRSETATIAALTAAGPRLVTSDGLSCT
                     SCHQIGSVPPAKAPLNARGPDISMLEKRIRREWYDRWCDNPARIVPRMEMPAVKIPVR
                     GVLNEKVSDQLAAVWHILNTPGFEPPEPNPVRVLRQSGLPGE"
     gene            complement(2325..5048)
                     /locus_tag="VFB80_18785"
     CDS             complement(2325..5048)
                     /locus_tag="VFB80_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35887.1"
                     /translation="MYLPLILSLLASSPSDVKLPTAAGAGEVFVCDFEEATDRDYDGW
                     PDRWVRSRGRELPEFLRIEIVPDPAAANRCLQITLDGGGAIVSSPPVAVSSHFSFLLT
                     CRLKTAGLKHDAAWLALSLLDAEGNVLQSHSSPPLTAAPDWQAVRIGPIAAVSPRAVR
                     AVIALHLQPLDQREDLTGRAWFDDVRIVRLPRMLLSSNLAAGICTSRDTPELVCEVSG
                     IRVRNPQVRFELFDQAGQRLARHDQSLLSPDEVAKAAEMGLPESGYAGRARWQPPLSA
                     FGYYKVRASLVEENGDEVLLDRTQSIAMVRQLPPPLRSAFGWTLEGAEQPYAYGPLTA
                     LLAQSGCGWAKMPVWYDARENKAADRIAWFAEQLSIQGIELVGVLDQPPADLRQVFRE
                     PGRLPVATVFAEPELWQPSVAPVMTRLSLKVRYWQLGNDQDVSFIGYPQLGPKVADIK
                     RRLEEYTQQIHLGLNWRWMYGPPPEQLAGGPWSFLSYGVDPPLTAEEIGAYLQKPAAA
                     RAVAQRPRSALPTSTGPQTARSVRPTFGPRREWSGPQRWMLLSPLPKGEYPAEVRVRD
                     LVLRMLAAKMHRADAVFVPQPFSDEEGVMNADGSPGELLVPFRTTAMLVGGAEYLGAI
                     QMPGGNAGHLFAQDGRAVMAIWSHRETTERVAIGDEIEQLDVYGRGVKPPHSEQDGQS
                     LVELKIGPMPTFVIGLREAVARFQAAAEFESTQLASVAGREQTLYLRLANTLHQPISG
                     ELTLHAPKSWGIDTRATRFRIGEGETLRLPLPVTLAADANSGPQPVRLDFELSGQGGS
                     SRFSVYRTLQLGLDDVQVELTTRLRAKDGALVAELHLANQADFPLSFRCVLFAPGRRR
                     ETRQVIDLGRERTTLTYVLPRGEELIGKKLWLQAEEIGGSRVLNHTLTAER"
BASE COUNT          809 a         1724 c         1755 g          815 t
ORIGIN      
        1 atctcgcccg gcaagccgct ttgccgcaac acgcggacgg ggttgggctc tgggggctcg
       61 aagcctgggg tgttgaggat gtgccagacg gcggcgagct ggtcggaaac tttttcgttc
      121 agaacgccgc ggacgggaat cttcaccgcg ggcatttcca tccgcgggac gatccgcgcg
      181 gggttgtcgc accagcggtc gtaccactcg cggcggatgc gcttttcgag catcgagatg
      241 tccggcccgc gggcgttcag tggcgccttg gccggcggga ccgagccgat ctggtggcag
      301 ctcgtgcagg acaggccgtc ggaggtaacc agccgcgggc cggcggctgt caaagccgcg
      361 atagtagccg tctcgctccg cgagacgctt gcctcacgcg gagcgtgagg gctactttcg
      421 ggcacgcggt cggtggcggt gaaataggcc gtcaagtcgg cgagctgggc atcgctcagc
      481 gcaaacttcg gcatttgcac gagcaggtag ggccggtgcg gcggaccctg gcggcggatc
      541 gaatcggcca gggccgcatc gagcagcttg tcgcccacgc cattgagcgc cggcggcgtc
      601 atcgcaggga ctagcggcgc tagggcagcg tgattttccg cgacggcaac aagtctgtct
      661 tgcaaagcag caggcacact ccctgtgccg tcattcttcg aaatgggctc attcgcgcgc
      721 gacggcacac ggagagtgcc tgctacatta cgctcgatgc cttcgcgctg atggcaggcc
      781 aggcagttga gctgcaccag caaatcccgc ccgcgagctt gcggtgagag ctttgtcccc
      841 ggtcgcggct gcgagtagta gactacgagc gcggcgcggt cttcgtcggc gagccgatag
      901 ccgggccggc ctttcgcccg gtcggcattt gtggcacagg agctcgacca attgctcttg
      961 gccgtcagcg gagccagtga ggctgccgcc aggccggcgt cattacgctt caggacgtgg
     1021 cagttcgcac agcgcagcga agtcacgagc cgctcggcct cgcccgctcg cccggcggaa
     1081 ccagcggcgg gctttgcgcc ttgctcctgc caccacaggt cgagcgactt cagctcgtct
     1141 ggcgtgaacg tgaactgcgg cattcggtgc gagcggttga tcgccgcggg atcactcagc
     1201 cagtgaatca gaaagtcggc cggccgcttt tccgccagct tcgtcaggtc gccgccgcca
     1261 aacaggccgc tctcgcccag ttcgcccagc tggtgacaag ccaggcagcc gcgggtcagc
     1321 aagagccgct gaccctctcc agccgtgggc ttgggcggct cctcttcgcc ctttttcttc
     1381 ccgttcttgg acttcgcttt cgccggccga tcggcgggca gtttcacttc caccttcgtc
     1441 gccggctcgc gcagcagcca cgccgcaaca gccgccgctt cgtcgcgcgt caggcccagg
     1501 tcgggcatgc gccgcggcga ggagccggcg gcatgattgc taagccaatc gacgagccac
     1561 gcttcatgga cgtttccgct gagccgatca agcgccggtg cgggcgtgac gctggccgac
     1621 tgatcccggt ggcaggcggc gcagcggagc gccgccgcta gctgccggcc gcgctcgaat
     1681 tccgacgagg ggctccgctc cttgttgtgc gcgagaaacc gcgccgcgat cggttccagg
     1741 cggaattccg gtccggtcca gaacagggcg agttgcccgc gcgggcccga gcggcgatac
     1801 gtgatctcca gttcgtgccg gtcgaacgtc aactccagcg gctgcgagct gagccactgc
     1861 ggcgactcgg cctgccccgt cagcacggtt ttgcccgcca gcttcacctc gacgctgccc
     1921 tgggcaaagc aaagcagctg gtacttgccg tccgcttggg cccacagtcg cccgcgccaa
     1981 attgccgaga actcaccggc cggcagcctg ggatcgggcg cggcgtcttg ccagtcgaag
     2041 gcgacggttt catccagccg cgttgccgcc agcccggcgg cagaataggt tgcgaccaaa
     2101 ccgggcctgt agggattctc ttcgtccgcc tcttcctggg catttgccgg cagcgcgcca
     2161 agcagcagta gcatcagaac ccaaaaccga acgctcatta ttggcaactc cgcgtgggat
     2221 tttgccacga taacacggcg gccggtcagt cggttagtcg gtggtcggtg agtcggtata
     2281 gcaccgacct agcgactgac cgatcaccga ccgaccgatt tccgctaccg ctccgcggtc
     2341 agcgtgtggt tgagcacccg cgagccgccg atttcttcgg cctggagcca cagcttttta
     2401 ccgatcagct cctcgccccg cggcaggacg taagtcagcg tggtccgctc gcggccgagg
     2461 tcgatcacct gccgcgtctc gcggcggcgg cccggcgcga agagtacgca gcgaaagctc
     2521 agcgggaaat ccgcctggtt cgccaggtgc aactcggcga cgagcgcccc atccttggcc
     2581 cgcaggcgcg tggtcagctc gacctgcacg tcgtcgaggc ccagctgcag cgtgcgatag
     2641 acgctgaagc gggagctgcc gccttggcct gacaattcaa aatccagccg cacgggctgc
     2701 gggccgctgt tggcgtcggc ggccagcgtg accggcagcg gcagtcgcag tgtctcgccc
     2761 tcgccgatgc ggaaccgcgt ggcccgcgtg tcgatgcccc agctcttggg cgcgtgcagg
     2821 gtcagctcgc cgctgattgg ctgatggagc gtgttggcca ggcgcaggta gagcgtctgc
     2881 tcgcggccgg cgacgctggc cagctgcgtc gattcgaact cggccgcggc ctgaaagcgg
     2941 gccaccgcct cgcgcaggcc gatcacgaac gtcggcatcg gcccaatctt cagctccacg
     3001 agggactgtc cgtcctgctc gctgtggggc ggcttaacgc cgcggccgta aacgtcgagc
     3061 tgctcgatct cgtcgccgat ggccaccctc tcagtcgtct cgcggtggct ccagatcgcc
     3121 atcacggccc ggccatcctg ggcgaacaag tgcccggcgt tgccgcccgg catttggatc
     3181 gcccccagat actccgcgcc gccgaccaac atggccgtcg tgcggaacgg caccagcagc
     3241 tcgcccggcg agccgtcggc gttcatcacg ccttcctcgt cgctgaacgg ctgcggtacg
     3301 aacacggcgt cggcgcggtg cattttggcc gccagcattc gcagcaccag atcgcggacg
     3361 cgaacttcgg cgggatattc gcctttgggc agcggcgaga gcagcatcca ccgctgcggc
     3421 ccgctccact cgcggcgcgg gccaaacgtc ggccgcaccg agcgggcggt ctgggggccg
     3481 gtgctcgtcg gcagcgcgga ccgcggccgc tgggcgaccg cccgcgcggc cgccggcttc
     3541 tgcaaatagg ccccgatttc ttcggcggtc agcggcggat cgacgccata cgaaaggaac
     3601 gaccagggtc cgcccgcgag ctgctcgggc ggcgggccat acatccagcg ccagttcagc
     3661 ccgaggtgaa tctgctgcgt gtattcttcc agccgccgct tgatgtcggc caccttcggg
     3721 ccgagttgcg ggtagccgat gaagctcacg tcctggtcgt tgccgagctg ccagtagcgg
     3781 accttcagcg acagccgcgt catcaccggc gcgaccgacg gctgccagag ctccggctcg
     3841 gcgaagacgg tggccacggg caggcggccc ggctcgcgga agacctgccg caggtcggcc
     3901 ggcggctgat cgagcacgcc gacgagctcg atgccttgaa tgctgagctg ctcggcgaac
     3961 caggcgatgc ggtcggccgc tttgttttcg cgggcgtcgt accagaccgg catcttcgcc
     4021 cagccgcacc ccgactgggc cagcagggcg gtcagcggac cataggcgta gggctgctcg
     4081 gcgccctcca gcgtccagcc aaaggccgag cggagcggcg gcgggagctg gcggaccatc
     4141 gcgatcgact gcgtgcggtc gagcaggact tcgtcgccgt tctcttcgac cagcgacgcc
     4201 cgcaccttgt agtagccgaa ggccgagagc ggcggctgcc agcgcgcccg cccggcgtaa
     4261 cccgattcgg gcaggcccat ttcggccgcc ttagcgactt catcgggcga gagcagcgac
     4321 tggtcgtgtc gggccagccg ctggccggcc tggtcgaaca gctcgaaccg cacttgcgga
     4381 ttgcggacgc gaatgccgga gacttcgcag accagctcgg gcgtatcgcg gctggtgcag
     4441 atgcccgcgg ccaggttgga cgagaggagc atccgcggca agcgaacgat gcgcacgtcg
     4501 tcgaaccagg cccgcccggt caggtcttcg cgctggtcga gcggctgcaa gtgcagcgcg
     4561 atgacggccc gcacggctcg cgggctgacg gcggcgatcg ggccgattcg gaccgcctgc
     4621 caatcggggg ccgcggtcaa tggcgggctg gaatgcgact ggagcacgtt cccctcggcg
     4681 tcgagcagcg agagcgccag ccaggcggcg tcgtgcttga ggccggcggt cttcaggcgg
     4741 caggtgagca gaaagctgaa gtgcgagctg acggccaccg gcgggctgga gacgatcgcg
     4801 ccgccgccgt cgagggtgat ttgcaggcag cgattggcgg cggccgggtc gggaacgatc
     4861 tcgatccgca gaaattccgg cagctcccgg ccccgcgagc ggacccagcg gtcgggccag
     4921 ccgtcgtagt cgcggtcggt cgcctcctcg aagtcgcaga cgaacacttc gcccgcgccc
     4981 gccgccgtcg gcagctttac gtcgctgggc gacgaggcga ggagcgataa aatgaggggc
     5041 agatacataa gaaccaaaga accaaagaac cgaagaaccg aagaaccaaa gaaccgaaga
     5101 acc
//
LOCUS       DASRJB010000710         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1055687, whole genome shotgun sequence.
ACCESSION   DASRJB010000710 DASRJB010000000
VERSION     DASRJB010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055687"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFB80_18790"
     CDS             <1..1351
                     /locus_tag="VFB80_18790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN35888.1"
                     /translation="LREDLKEIELYFDTDEADYWQVLTEELDRKAQGHDFHQWFVQFY
                     GADGRPTWHSQQAPQLPDFTADQRRLRTFSVRDLRLSYLPLPLPVPEASAVCVGCSQK
                     PLARDMQSIDRLVLAVGFVVLLISPVAGHLLTNRTIRPLAQMIRTTARLRPGELGQRM
                     PIRGTGDELDSLARTINALLDRISSYLKQEHDFLANAAHDLRTPLAAIRSSVEVALSG
                     KRTEAEYRELLGVVIEQCTALQTLVNQLLLLAETDADRLQTDAEPVALDQVVAGACEM
                     FEAVAEFHGIELRVMSLAPVAVEGNRHHLRQVVNNLLDNAIKFTAKEAPESAEPASGS
                     SRQGKIDVELSRDDERREVRLRITDSGQGIDAEHLPLIFERFYRADRARARDGVSGGS
                     GLGLSICKAIVEAHRGTIAVRSKPGQGSTFTVTLPLSTRDADRQPADDRRAAVGTSS"
     gene            1402..>3360
                     /locus_tag="VFB80_18795"
     CDS             1402..>3360
                     /locus_tag="VFB80_18795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HZN35889.1"
                     /translation="MSAEPLERHLQPFQLTAFRPGQRDVIAAVLAGRDCLCIMPTGGG
                     KSLCYQLPAVAREGLTLVVSPLIALMKDQVDGLTRLGIAATYINSSLAPAEAFQRIDQ
                     MAAGRYDLVYIAPERLRSSLFLEKLRETKLQLLAVDEAHCISQWGHDFRPDYARLGRF
                     REKLGYPQTIALTATATQGVQADVAQQLRLREPQVFITGFARPNLSFEVQPVLSEIDH
                     ERSLTGFLQTTPGAGIIYCATRKRCEELAPIASAVSGRRADLYHAGLEPDVRRRVQDD
                     FRSGKTPIIVATNAFGMGIDKPDLRFVVHYNIPGTLEAYYQEAGRAGRDGDPARCVLF
                     YSQRDRRIQEFFIDSAYPEPEIVEQVYNFLRQIDHDPIELTLDEIRETLGLNIRSEGV
                     AACEKLLAGVGALERLDSRQNMAAVRIDSDLPSLVDLLPKQAKMQRRVLQAVEREVGG
                     RRFERVYFPPRKIASMAELDPAAISQALKELSKLQAFDYVPPFRGRAVHMLIKDKEFD
                     DLGIDFDEQQRHKADEYAKLDRMIAYAEAHSCRQLEILDYFGDPNREACGSCDNCLVR
                     AKNQAGGASPASGRRQLPDDSESPGPRHREADASRSPAVVEAVRIVLSGAARLKGRYG
                     KQMLAKMLVGSQAKEVTKFRLNQLSTFGL"
BASE COUNT          572 a         1169 c         1104 g          515 t
ORIGIN      
        1 gctccgcgag gatctcaagg agatcgagct ttacttcgac accgacgagg ccgactattg
       61 gcaggtgctc acggaggaac tcgaccgcaa ggcgcagggg cacgactttc accagtggtt
      121 cgtgcagttc tatggcgccg atgggcggcc gacctggcac agccagcagg ccccccagct
      181 gcccgacttc accgccgatc agcgccgcct gcggaccttc agcgtccgcg acttgcggct
      241 gtcgtacctg ccgctgccgc tgccggtgcc cgaggcgtcg gccgtctgcg tgggctgctc
      301 gcagaagccg ctggcccgcg acatgcaaag catcgaccgg ctggtgctgg cggtcggctt
      361 tgtcgtcttg ctgatctcac ccgtcgcggg ccacttactc accaatcgca cgatccgccc
      421 gctggcgcag atgatccgca cgacggcccg gctgcggccg ggcgagctgg gccagcgaat
      481 gccgatccgc ggcacggggg acgagctgga cagcctggcc cgcacgatca acgccctgct
      541 cgaccggatc tccagctacc tcaagcagga gcacgacttt ctggcgaacg cggcccacga
      601 cctgcggacg ccgctggccg cgatccgcag cagcgtcgag gtggctctca gcggaaagcg
      661 gacggaggcc gagtaccgcg agctgttggg cgtggtcatc gagcagtgca ccgccctcca
      721 gacgctcgtc aaccagctcc tgctcctggc cgagaccgac gccgaccggc tgcaaaccga
      781 cgccgagcca gttgcgctcg atcaggtcgt ggccggcgcg tgtgaaatgt tcgaggcggt
      841 cgccgagttc cacggcatcg agctgcgggt gatgagcctc gcgcccgtcg ccgtcgaggg
      901 gaaccgccat cacctgcggc aggtcgtcaa caacctgctc gataacgcca ttaagttcac
      961 tgccaaggaa gcgcccgagt cggcggagcc tgcgagcggg tcttcccggc aaggcaaaat
     1021 cgacgtcgaa ttgtctcgcg acgacgagcg gcgggaggtc cgcctgcgga tcaccgacag
     1081 cggccagggc atcgacgccg agcacctgcc gctcatcttc gagcgcttct atcgggccga
     1141 ccgggcccgg gctcgcgacg gcgtcagcgg cggctccggc ctgggcctga gcatctgcaa
     1201 ggccatcgtc gaggcccacc gcggcacgat cgccgtccgc agcaaacctg gccaaggctc
     1261 gactttcacc gtcacgcttc cgctctcgac gcgggacgcg gatcgccagc ccgcggacga
     1321 ccgccgggcg gccgtcggaa cttcgtcgta gggtgctgaa gaacggccac ggtacccaaa
     1381 cgctcccgct acaatcgcgg catgtccgcg gagccacttg aacgtcactt gcagcctttc
     1441 cagctcaccg ctttccgccc gggccagcgg gacgtgatcg cggcggtgct ggccgggcgt
     1501 gactgcctgt gcatcatgcc gaccggcggc ggcaagagcc tctgctatca attgcccgcc
     1561 gtcgcccgcg agggactgac gctcgtcgtc tcgccgctca tcgccctgat gaaggaccag
     1621 gtggacggcc tgacgcggct gggcatcgcg gcgacctaca tcaacagctc gctcgccccg
     1681 gccgaggcct ttcagcgcat cgaccagatg gccgccggcc gctacgacct ggtttacatc
     1741 gccccggagc ggctccgcag ctcgctgttt ctcgaaaagc tccgcgagac aaaactccag
     1801 ctcctcgcgg tggatgaggc ccactgcatc agccagtggg gccacgattt ccgccccgac
     1861 tacgcccggc tcggccggtt tcgcgaaaag ctgggctatc cgcagacgat cgccctcacc
     1921 gccaccgcca cgcagggcgt gcaggccgac gtcgcccagc aattgcggct ccgcgagccg
     1981 caagtcttca tcaccggctt cgcccggccg aacctgagct tcgaggtgca gccggtgctg
     2041 agcgagatcg accacgagcg gtcgctgacc ggctttttgc aaacgacgcc cggggcgggc
     2101 atcatctact gcgcgacgcg gaagcggtgc gaggagctgg cgccgatcgc gagcgccgtc
     2161 agcggccggc gggcggacct gtatcacgcg gggctggagc ccgacgtccg ccgccgcgtg
     2221 caggacgact tccgcagtgg caagacgccg atcatcgtgg ccacgaacgc ttttggcatg
     2281 ggcatcgaca agcccgacct gcggttcgtg gtgcactaca acattccggg cacgctggag
     2341 gcctattacc aggaggcggg ccgcgccggc cgcgacggcg accccgcgcg gtgcgtgctg
     2401 ttctattcgc agcgcgaccg gcgaatccag gagttcttca tcgacagcgc ctatcccgag
     2461 ccggagatcg tcgaacaggt ctacaacttc ctccgccaga tcgaccacga cccgatcgag
     2521 ctgacgctcg acgaaatccg cgagacgctg ggcctgaata tccgcagcga aggggtggcc
     2581 gcctgcgaga agctgctggc cggcgtcggc gcgctggagc ggctcgattc gcggcagaac
     2641 atggccgccg tgcggatcga cagcgacttg ccgtcgctcg tcgatctgct tccgaagcag
     2701 gccaagatgc agcgccgcgt actgcaggcg gtcgagcggg aagttggcgg gcggcggttc
     2761 gaacgggtct attttccgcc ccggaaaatc gcttccatgg ccgagctaga ccctgccgcc
     2821 ataagccagg cgctcaagga gctgtcgaag ctccaggctt tcgactacgt cccgccgttc
     2881 cgcggccgcg cggtgcacat gctgatcaag gacaaggaat ttgacgacct ggggatcgac
     2941 ttcgacgagc agcagcggca caaggccgac gaatacgcca agctcgaccg gatgatcgcc
     3001 tatgccgagg cgcattcctg ccgtcagctg gagatcctcg actatttcgg cgaccccaat
     3061 cgcgaggcgt gcggaagctg cgacaactgc ctcgtgcggg cgaagaacca ggctggcggg
     3121 gcgagcccgg cgagcgggag gcgtcagctt cccgatgatt ctgaatcgcc agggccgcgt
     3181 catcgggagg ctgacgcctc ccgctcgcct gccgtcgtgg aagccgtgcg gatcgtcctc
     3241 tccggcgcgg cccgcctgaa gggccgctac gggaaacaga tgctggccaa gatgctcgtc
     3301 ggctcgcagg ccaaggaagt cacgaaattc cggctcaacc agctcagcac gttcggcctg
//
LOCUS       DASRJB010000711         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1331456, whole genome shotgun sequence.
ACCESSION   DASRJB010000711 DASRJB010000000
VERSION     DASRJB010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331456"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..583)
                     /locus_tag="VFB80_18800"
     CDS             complement(32..583)
                     /locus_tag="VFB80_18800"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN35890.1"
                     /translation="MQSQAATVEEYLAELPQDRRLALSAIREVILKNLDKDIEEGMQY
                     GMIGYYVPHRVYPAGYHCDPQQPLCYAGLASQKNHISLYMMGVYCDTSEHKRWFVEAW
                     KKTGKKLDMGKCCIRFKNLEGVPLEVVGQAIKRMPAKKFIAIYEEAKETVFKRESKPR
                     AKAKARPKARPVKARAKRATAKS"
     gene            727..1779
                     /locus_tag="VFB80_18805"
     CDS             727..1779
                     /locus_tag="VFB80_18805"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HZN35891.1"
                     /translation="MSTRRLIAPLLALTVVLSATAAWSYYRLAVTGETMGEAATKFLA
                     ALSAEQRAKAALPYDDARRSAWHFIPLEGAKEREGLKVREMSPEQRTAAFALLKSALS
                     EVGYGKATKIMELESLLLELQKPKAAPIRDSERYYFVVYGAPTSEGRWGLSIEGHHLS
                     LNFVVDKGKVISSTPTAFCANPAIVMNENVPSIKKGTRLLAKEETLAFELLASLSPEQ
                     KKVAVIADKALGEVRSAGEPQPPTDAPAGLAAEKLTGQQRGILQSLLDAYCENLPADV
                     VKERREAIEKQGLGQVHFAWAGAQQPGVGHYYRVQGPTFLIEFVNTQPDSAGNPANHI
                     HSMWRDMQGDFALPIK"
     gene            1946..3166
                     /locus_tag="VFB80_18810"
     CDS             1946..3166
                     /locus_tag="VFB80_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN35892.1"
                     /translation="MNHASHGPEHAHPPQREPGPSTLAVHAGEQRQKAVHSITDPIVC
                     ASTYTFPNTQAVIDYIEQKQDREEYGRYGNPLEKVVERKLAALEGGEEAILYASGMAA
                     IVGLLMAKLNAGDEVVFFDECYHRSREFCNKHLSRFGVVTQQVKACDYAAMSAAINPR
                     TRLLISESPTNPHLSVVDLERFAEIGRRHKVPTLIDATLATPYNVRPIACGVDYVLHS
                     ATKYLGGHNDLLAGVIIGRKSDLESVRKLRGIMGAVNSPHNMYLLERGLKTFELRMQR
                     HNANGQAIAEFLASHPRVERVYYPGLPGHPYYDVARRQMRGFGGLVTFLVKDADWRQT
                     AAIVDRVKIARIAPSLGGVESLIEQPLVMSYYEVPPDDRRRFGIPDNMIRLSCGIENA
                     EDLIEDLRQALEAK"
     gene            3204..3461
                     /locus_tag="VFB80_18815"
     CDS             3204..3461
                     /locus_tag="VFB80_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35893.1"
                     /translation="MRRFKWIEWNVGKVEGHALAIEEVEAAFDRVFSLEERKDGSFQM
                     FAETPGGRRIWVIWRFDREDDELPDIFGEVVGAPIFVITAY"
     gene            3472..3720
                     /locus_tag="VFB80_18820"
     CDS             3472..3720
                     /locus_tag="VFB80_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35894.1"
                     /translation="MTAPAADNRSPARRALDEHIAAEPPLPPLPVPAGASPVEAHLAR
                     RVRRPVAVAGIVQNGVVRPIDPDVKLPELSRVIIVAAE"
     gene            3761..4903
                     /locus_tag="VFB80_18825"
     CDS             3761..4903
                     /locus_tag="VFB80_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZN35895.1"
                     /translation="MRFRTRAIHVGQERDPQTGAVVPPLYVAATYVQPGAGVWAEFDY
                     SRSGNPTRKNFERAIADLEGGCGGLAFASGMAATHCAMLLLSSGDHVVAGADIYGGSY
                     RLLHKVLNHSNIGVTLAAGTDLAALEDAITPQTRLVWIESPGNPQMSITDIAAVAAIA
                     KRRGVLLGVDNTFATPVLTRPLELGADIVMHSATKYIGGHSDLLGGALVVKDKTLFDK
                     LYFLQNATGGVMGPLECFLASRGLKTLELRVREQSRTAQQLAEWLAHDPRISRVLYPG
                     LPGHPGHEIARRQMDGAFGAMLTIEVRGDFAKAKRVCEATQLFQLAVSLGAVESLIEQ
                     PASMSHASYDAAARAAHGITDGLIRLSVGLEAFEDLQGDLSQALDA"
     gene            complement(4913..5692)
                     /locus_tag="VFB80_18830"
                     /pseudo
     CDS             complement(4913..5692)
                     /locus_tag="VFB80_18830"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT          995 a         1876 c         1890 g         1031 t
ORIGIN      
        1 aaggatttca agaagatcaa ggacgatatg ctcaagactt ggcggtcgcc cgtttcgccc
       61 gcgccttgac cggccgggcc ttgggcctgg ctttggcctt cgcccgcggc ttactctcgc
      121 gcttgaacac cgtctccttg gcttcctcgt agatcgcgat gaacttcttc gccggcatcc
      181 gcttgatggc ctggccgacg acctccagcg gcacgccctc gaggttcttg aagcgaatgc
      241 agcatttgcc catgtcgagc ttcttgcccg tcttcttcca ggcctcgacg aaccaccgct
      301 tgtgctcgct ggtgtcgcaa tagacgccca tcatgtacag cgagatgtgg ttcttttgcg
      361 aagccagccc ggcgtagcac agcggctgct gggggtcgca gtggtagccg gcgggataga
      421 cgcggtgcgg cacgtaatag ccgatcatgc cgtactgcat gccctcctcg atgtccttgt
      481 cgaggttttt caggatcacc tcgcggatcg ccgacagggc caggcggcga tcctgcggca
      541 gttcggcgag gtactcttcg acggtggcgg cctggctttg cataatgcgg caagtgtagg
      601 gcgcagcgga agtgaatgac aagtatcggc tggtggccgc cgaggcggcg cttttatcgc
      661 cactgggccg cgggtatatt ccagtcaggc tcgctaccgc gtcccgcctc cctggagacc
      721 tgcccgatgt ccacccgccg cctgattgcc ccgctgttgg cattgacggt tgttctttcg
      781 gccacggccg cctggtcgta ttaccggctg gcggtgacgg gcgagaccat gggggaggcc
      841 gcgaccaagt tcctggccgc gctgtcggcc gaacaacggg ccaaggcggc gctgccctac
      901 gacgatgccc gccgcagcgc ttggcacttt atcccgctgg aaggcgcgaa agagcgcgaa
      961 gggctcaagg tccgcgagat gtcgcccgag cagcggacgg ccgcgttcgc cctgctcaag
     1021 tcggccctca gcgaagtcgg ctacggcaag gcgacgaaaa tcatggagct ggagagcctg
     1081 ctgctggaat tgcaaaagcc caaggccgcc ccgatccgcg actccgaacg gtactacttc
     1141 gtggtgtatg gcgcgccgac cagcgagggc cgctggggcc tgagcatcga ggggcatcac
     1201 ctctcgctga acttcgtcgt cgataagggc aaggtcattt ccagcacccc gactgccttt
     1261 tgcgccaacc cggcgatcgt catgaacgag aacgtgccct cgatcaaaaa ggggactcgt
     1321 ctgctggcga aggaggagac gctggccttc gagcttttgg cctcgctctc gcccgagcag
     1381 aagaaagtcg ccgtcatcgc cgacaaggcc ctgggcgaag tccgctcggc cggcgaaccg
     1441 cagccgccca ccgacgcccc cgcgggcctg gcggcggaga agctcacggg tcagcagcgc
     1501 ggcattctgc agtcgctgct cgacgcgtat tgcgaaaacc tgccggccga cgtggttaag
     1561 gagcgccgcg aggcgatcga gaagcaaggc ctcggccagg tccacttcgc ctgggccggc
     1621 gcacagcagc ctggcgtggg ccactactac cgcgtgcaag gcccgacgtt cctgatcgaa
     1681 ttcgtcaaca cgcagcccga ttcggccggt aatccggcca atcacatcca cagcatgtgg
     1741 cgcgacatgc agggcgattt tgccctgccg atcaagtagc ttgtggccgg ccgcggggca
     1801 agctatagtt ccttcaccag ggatttgcgg ccgcttgcag cctccaactg ctaaagtgcc
     1861 atcatcggtc ccgccttggg actcttgaat cgcccagctt cgggcggccc ggcaagttcc
     1921 tccgttatcg gagctttcgc cggccatgaa ccatgcttcc cacggccccg agcacgcgca
     1981 ccctccccag cgcgagcccg gcccttcgac gctcgccgtc cacgccggcg aacagcggca
     2041 gaaggccgtc cactcgatca ccgacccgat cgtctgcgcc tcgacctata cctttccgaa
     2101 cacgcaggcc gtcatcgact acatcgagca aaagcaggat cgcgaggaat acggccgcta
     2161 cggcaacccg ctcgaaaaag ttgtcgagcg caagctcgcg gcgctggaag gcggcgaaga
     2221 ggcaatcctc tacgccagcg gcatggcagc gatcgtcggc ctgctgatgg cgaagctcaa
     2281 cgccggcgac gaggtcgtgt tcttcgacga gtgctatcac cgcagccgtg aattctgcaa
     2341 caagcacctg tcgcggttcg gcgtcgtcac gcagcaggtg aaggcctgcg actatgcggc
     2401 gatgtccgcg gcgatcaatc cgcggacgcg gctcttgatc agcgaatcgc ccaccaatcc
     2461 ccacttgagc gtcgtggatc tggagcggtt cgccgagatc ggccggcggc acaaagtccc
     2521 gacgctgatc gacgccacgc tggccacgcc ctacaacgtg cggccgatcg cttgcggcgt
     2581 cgattatgtg ctgcattcgg cgacgaagta cctgggcgga cacaacgacc tgttggcggg
     2641 cgtgatcatc ggccgcaagt cggacctgga gagcgtccgc aagctccgcg ggatcatggg
     2701 cgctgtcaat tcgcctcaca atatgtacct gctggagcgc ggcctgaaga cgtttgagct
     2761 gcggatgcag cggcacaacg ccaatggtca ggcgatcgcc gagttcctgg cgtcgcaccc
     2821 gcgcgtggaa agagtctatt atcccggctt gcccgggcat ccgtactacg acgtcgcccg
     2881 ccggcagatg cgcggtttcg gcggactcgt gacgtttctg gtcaaggacg ccgactggcg
     2941 gcagacggcg gcgatcgtcg atcgggtcaa gatcgcccgc atcgccccca gcctgggcgg
     3001 cgtcgagtcg ctgatcgagc agccgctggt gatgagctac tacgaagtcc cgccggacga
     3061 ccgccgccgc ttcggcatcc cggacaacat gattcgcctg tcgtgcggca tcgagaatgc
     3121 cgaggacctg atcgaggacc tgcggcaggc gctggaagca aagtgaagcg gcccgcgtac
     3181 atctgtgcga aaggcgctgg cgcatgaggc gattcaagtg gatagaatgg aatgtcggca
     3241 aagtcgaagg gcacgcgctt gcgatcgagg aagtagaggc ggcgttcgat cgagtcttta
     3301 gcttggaaga gcgtaaggac ggctcctttc agatgttcgc ggagacgccg ggcggtagaa
     3361 gaatttgggt catttggaga tttgatcgcg aagatgatga gcttcccgac atttttggcg
     3421 aagtcgtggg cgccccaatc ttcgttatca cagcctatta atgagtgacg catgacagct
     3481 cccgcagcag acaatcgcag ccccgcgcgg cgggcattgg acgagcacat tgccgctgag
     3541 cccccgctgc cgccgctgcc agtgccggcg ggggcaagtc ccgtcgaagc gcatctggcg
     3601 cgccgcgtac ggcgtccagt ggccgtggcc ggcatcgtgc aaaacggcgt cgtcaggccc
     3661 atcgatccag acgtcaagct gcccgaattg tcccgcgtga tcatcgtcgc ggccgagtag
     3721 ccccatagtg aaacctgctc tgcatcgggc attgcactcc atgcgttttc gcacccgtgc
     3781 cattcacgtc ggccaggagc gcgatccgca gaccggggcg gtggtgccgc cgctgtatgt
     3841 ggcagcgacc tacgtgcagc cgggcgcggg cgtctgggct gagttcgact attcgcggag
     3901 cggcaatccg acccgcaaga acttcgagcg cgcgatcgcc gacctggagg gaggctgcgg
     3961 cggcctggcg ttcgcttcgg gcatggcggc gacgcactgc gcgatgctgc tgttgagctc
     4021 cggcgaccac gtcgttgccg gcgccgatat ctacggcggc tcctatcggc tgctgcacaa
     4081 agtgcttaac cacagcaaca ttggcgtgac gctcgcggcc ggcaccgacc tggcggcgct
     4141 ggaagacgcg atcacacccc agacgcggct ggtatggatc gagagccccg gcaacccgca
     4201 gatgtcgatc accgacatcg cggcggttgc ggcgatcgcc aagcgccgcg gcgttctgct
     4261 cggcgtggac aacacgttcg ccacgcccgt gctcacgcgt ccgctggaat tgggcgccga
     4321 catcgtgatg cactccgcga ccaagtatat cggcgggcac agcgatctct tgggcggcgc
     4381 cctggtggtc aaagacaaga cgctgttcga caagctctat ttcctgcaga acgctaccgg
     4441 cggcgtgatg ggaccgctgg agtgctttct cgccagccgt ggcctcaaga cgctggagct
     4501 ccgcgtccgc gaacagtcgc gcacggcgca gcagctggcc gagtggctgg cgcacgaccc
     4561 ccgcatcagc cgcgtcctct atcccggctt gccggggcat cccggccacg agatcgcccg
     4621 gcggcagatg gacggcgcgt tcggggcgat gctcacgatc gaagtccgcg gagactttgc
     4681 caaggccaag cgggtttgcg aggccacgca gctgttccag ctggccgtca gcctcggcgc
     4741 cgtcgaatcg ctgatcgagc agccggcctc gatgtcgcac gccagctacg acgcggccgc
     4801 ccgcgccgcc cacggcatca ccgacggcct catccggcta tcggtcggcc tggaggcttt
     4861 cgaagatctg cagggcgatc tgagccaggc gctcgacgcc tgaaatgtcc cgctagaaat
     4921 cgaactggtc gatgttggcc ttggtaaaag tcagcggctg accgagcagg atgttgtcgc
     4981 cggcgatcgt gaccgtgccc aggcggccgg cggcaagcga cgtatcgccg ggcttgaggc
     5041 ggccctcgta gagcgccttg gccgagtgga tcgtgaggta gcccaggtcc attgtgttcc
     5101 acaggacgac ggcttcggtg atgccttcgt ggacgtagcg cttgttgtcg ttgggcaggc
     5161 ccagcccgat caccttcacg tcgctgcggc gggactgctt caccgcctcg gccgcgccgg
     5221 gcacggcggg cgagcagatg gccatgatca gcttcacgtc gggatgggcg ttgaggaccg
     5281 tgttcgtctc gtcgaacgcc ttggcttgct tgtcgtcgca ggggcggagg gcggccatct
     5341 tgatctcggg gaacttctcc gcccggcggg cctcaatgtg cttttgccac tcgatcatgt
     5401 tggcggccgt gagcgaggcg gtgatgatcg cgaactcgcc tttgccgccg aggattcgcc
     5461 cggcgtgatc catgagcgtc gtgccgatgc cttgcggcgt ggcctgattg accagaaagt
     5521 cgcgggcgtc cggctcggcg tcggcgtccc acgtcaggac cttgatgccc cgctggcgag
     5581 ccttccgcag caccgacgag acgccggccc ggttctcgac tgcgaccgcg atcacatcga
     5641 cgccccgcgt gatccaagct tcgatcactt cgttctgctt cgccgggtcg ggatcggtgg
     5701 gcccgtgagc ggccggctgc ctctagaccg gcaaattcca ttggcccgca gcgggcgctg
     5761 cggctagaat ggccctcgga aaagtacgca ag
//
LOCUS       DASRJB010000712         8534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3590649, whole genome shotgun sequence.
ACCESSION   DASRJB010000712 DASRJB010000000
VERSION     DASRJB010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3590649"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFB80_18835"
     CDS             complement(<1..268)
                     /locus_tag="VFB80_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35896.1"
                     /translation="MIRTIAVVYSLALCLVAIGCSRTYSGPRRFALSGKVTVDNQPID
                     HGLISFLPDGEGGRVCGGPITAGSYTIPEEMGATEGKYKVKINWN"
     gene            complement(311..1345)
                     /locus_tag="VFB80_18840"
     CDS             complement(311..1345)
                     /locus_tag="VFB80_18840"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35897.1"
                     /translation="MAASSLPLRPQGQRRGFTLVELLVVIAIIGVLVALLLPAVQAAR
                     EAARRMSCSNKLKQISLAVHNYHDTFQVFPPQGLPVHGARHSWGWAPLTFPYMELQPL
                     YDALKPNIGIAPNSVIGSLPMAATNPLLQQRVASFICPSDTGPLLNPFYANPRNSNNP
                     ANHYAKSNYVCNQQVIAFASMFNQLPPPVCARIATITDGTSNVLMLGERALRLPIDKR
                     STGAVVWGLPDNNSDATTCFHPNHPINTRDPSDDFRANNYAQYASLARTPSNCNAHVA
                     TSNHPNGAMFSLCDGSVRFINQTIASNPIAYNNGGSGCTSTGSINVTGPGFVYQNLYW
                     INDGNATGDY"
     gene            1448..2380
                     /locus_tag="VFB80_18845"
     CDS             1448..2380
                     /locus_tag="VFB80_18845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN35898.1"
                     /translation="MIAATLESLADQFSVQQLRSFCVVFEKQSYSAAAKEIGLTVPTI
                     WEQVRTLEARYRAALFRRRGRRIEATPTAALLYDSLRPLLAGLDSTFALVSEAAGDHP
                     RTLTLVTGARAMFEDLTAPLREFRARHRQVALRLLDSGTRRAAELVATDKADLALALE
                     PGPGMVGREICVERAYRIEYLAIFPRRHPLAKSARLRLADLVAHPLVVGHDGTYGRQL
                     LDQALHRERLTEQVQIVAETDNSAFTTACVRAGLGVGVVAGRPAGALCRGLASRSLAP
                     HLGEAWIAFLWKRGRRLTATLQTLMQLIREQAGV"
     gene            complement(2346..3458)
                     /gene="ada"
                     /locus_tag="VFB80_18850"
     CDS             complement(2346..3458)
                     /gene="ada"
                     /locus_tag="VFB80_18850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HZN35899.1"
                     /translation="MLKTTPRPPKPALSPRAMWRIVERREASAAFYFGVRTTGVFCRP
                     GCSSRLPNRENVLFFPSAGDARAAGFRACKRCRPDLRNPAGELAKLIARACHMLDNEH
                     GTNSLAEVAAASGYSASQFHRLFRRVTGVTPKVYAAARRTDRARIALASGMPVTQAAY
                     AAGYRASSRFYDEAGKSLGMRPAAFRHQALGETIRLATAETSLGCVVVAATARGVCLV
                     QLGADADELLRSARRQFAQASVALGDAEFENWVRQVVSRIETPSEAGDLPLDIQGTAF
                     QRRVWDALRAIPPGTTATYSQIAASIRRPKAVRAVGSACAANPVAVAVPCHRAVRADG
                     GLGGYRWGLSRKRALLDRERESSGHATRRPARGSTA"
     gene            complement(3850..4824)
                     /locus_tag="VFB80_18855"
     CDS             complement(3850..4824)
                     /locus_tag="VFB80_18855"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZN35900.1"
                     /translation="MPKAIRFSQTGGPEVLRVEDVAAGEPGEGQARVRHTAIGVNFID
                     TYHRSGLYPLPLPSGLGSEAAGVVEAVGKGVAHIQAGDRVAYAGGPPGSYSEVRLMPA
                     DRLLKLPAGISDQQAAAMMLKGMTVQYLIRRTYKVQAGETVLWHATAGGVGLIACQWL
                     KALGVTVIGTVGSAEKAEIAKAHGCTHAIDYTREDFVQRVREITGGQRVPVVYDSVGK
                     STFAGSLDCLRPFGLLVSFGNASGAVPPFELGILAPKGSLYVTRPTLATYTATRSDLE
                     ATARDLFDVVLSGKVKIEVHRTYRLADAQQVHRDLEGRQTTGSVVMVP"
     gene            complement(4889..6064)
                     /locus_tag="VFB80_18860"
     CDS             complement(4889..6064)
                     /locus_tag="VFB80_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35901.1"
                     /translation="MDFRRSGQLDERRRRVLMDEIASPPSDDSDPAPAPGSPKGPVRA
                     YSPAVLSDRQPRVTDLLPVRPLWVAVAMLLGLTGIAAIEAIHIHAAALPLAPGGEQLA
                     ALDASSRGSLAAWYSSLLLATATAAALVVFAIRVHRVDDYRGSYRIWIWTTAALAWLS
                     LDAATGVHDALGLAVNAAAGQHGLGSSLAAGSTAMWLALYGLVLGTLGIRLAIEVWPS
                     LPSFAALLVAALLYLCAALLQLDMLAAATPLGQSVLQSTLTLLAHLALTTSIGLYARH
                     VYLDATGRLRVHIDPDKKRAAKGKGRAKLKVVKEDRVEDSPPAQKTAAAAPSRPAEPA
                     RFGAPASGAPKSGATISKSPSHYDNEDDDEEDASDYGGERLSRAERRRLKKLARRAA"
     gene            6341..6913
                     /locus_tag="VFB80_18865"
     CDS             6341..6913
                     /locus_tag="VFB80_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35902.1"
                     /translation="MVRIRRIRTWGALAAGVAIGLAIGGALAAGVTFGRWNSQTAGIP
                     GASDLRLKAMAAYGNDNFAVATGVIDNDVEGLYTLDFLTGDLQCYVVNPRNGALGGWF
                     KTNVAKDLPPEGTKKPSYLLVTGTFRVQGAAGNQKPAASLCYVVDGNTGNCVAYSFPW
                     GQSYSASGQVQSTPMQAVGKWKTRTVGVRE"
     gene            6932..7945
                     /locus_tag="VFB80_18870"
     CDS             6932..7945
                     /locus_tag="VFB80_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35903.1"
                     /translation="MTLNLSDYVVAGEIINRRPNMTHGWIALRGQDQPLLVQLTGNPS
                     PDLVGKRFRFQIPDERPPPDPPPPPLDAKQIAWQQIGPTGPMSVETTAEGKPRVRLQW
                     HSQNGHVLIEVNDPELEWVEDDEADAARQKELEAAEAGERGEDAEDSAALDLGVGLPD
                     EDEDDPYGLFPEGLDEQLAGEEETDEPAKRREPRDWSEVIPGIDDETKKMYEEWDEVT
                     EGTKDVPLTEVFDPPIKIYPAEQLAALDDATAEATLKELLQRLALHGVALAICEHFTP
                     RMAYRYLTEEILPEYGVHPRLPQIGWVQHYDTSEDCPECQARIDREWEERKGNSGGEP
                     DEA"
     gene            8065..>8534
                     /locus_tag="VFB80_18875"
     CDS             8065..>8534
                     /locus_tag="VFB80_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35904.1"
                     /translation="MASGTVPSVVPPPIAESLQREQVIQRQLGRTSLHVRLIDLASGL
                     AFWLVGVVVFFIAAAAFDHLIGLGTIGRVLGLVALVGGSSWYLVAVIWPLVARSINPE
                     YAARTIEEATPSLKNSLINFLMLRSERGKMKEIVFQALEQKAASDIAGVPVESTV"
BASE COUNT         1451 a         2730 c         2963 g         1390 t
ORIGIN      
        1 tgttccagtt gattttgacc ttgtatttgc cctcggtcgc gcccatttct tcgggaatgg
       61 tgtagctccc cgcggtaatc ggaccgccgc agacgcgccc gccttcgccg tcgggcagga
      121 acgaaatcag gccgtgatct atcggctgat tgtctacggt gaccttgccg gataaggcga
      181 accgccgcgg tccgctatag gtccggctgc acccgatggc gaccaggcaa agcgccagcg
      241 aataaacgac cgcaatggtt cgaatcatga tcctcaccac tcaccaccta ccgctcacca
      301 ctcaccaacc tcaataatcg cccgtggcgt tgccgtcgtt gatccaatac aggttctggt
      361 aaacgaaccc cggcccggtg acgttgatgc tgccggtgct ggtgcagccg ctgccgccgt
      421 tgttgtaagc gatggggttg ctggcgatgg tctggttgat gaaccgcacc gagccgtcgc
      481 agagcgagaa catcgcgccg ttgggatgat tgctcgtcgc gacgtgcgca ttgcagtttg
      541 aaggcgtgcg ggccagcgac gcgtactgcg cgtagttgtt ggctcggaag tcgtcgctgg
      601 gatcacgcgt gttgatcggg tggttggggt ggaagcaggt cgtggcgtcc gagttgttgt
      661 cgggcaggcc ccagaccacg gcccccgtgg agcgcttgtc gatgggcagc cgcagggccc
      721 gctcacccaa catcagcacg ttgctggtgc cgtcagtgat ggtggcaatc cgcgcgcaaa
      781 cgggcggcgg aagctggtta aacatggagg cgaaggcgat gacttgctgg ttgcagacgt
      841 aattgctctt ggcgtagtga ttggcgggat tgttgctgtt ccgggggttg gcgtagaagg
      901 ggttgagcag cggccccgta tcggacgggc aaatgaacga cgccactcgc tgttgcaata
      961 acggattcgt cgcggccatc ggcaatgagc caatgaccga gttcggcgcg atgccgatgt
     1021 tcggcttgag cgcgtcatag agcggctgca gctccatgta aggaaaggtc aggggcgccc
     1081 agccccaact gtggcgagcg ccgtgcacgg gcaggccctg cggcgggaag acctggaacg
     1141 tgtcgtgata gttgtgcacc gccaggctaa tctgcttcag cttgttgctg cagctcatgc
     1201 ggcgggcggc ttcgcgggcg gcctgtacgg cgggcaggag cagcgccacg agcacgccga
     1261 tgatcgcaat cacgaccagc agctcgacga gcgtgaagcc gcggcgctgg ccttgcggcc
     1321 ggaggggaag ggaggaggca gccatggtcg aacctgggga gaagctggcg gggagttccg
     1381 ctggatgata cccgaggtat tattcagtac aatcaaatat ttgcgaaaca ttgtaaggga
     1441 cagttgaatg atcgccgcga cgctcgaatc gctggccgac caattctccg tccagcagct
     1501 ccgctcgttc tgcgtcgtgt tcgagaagca gagctattcg gcggccgcca aggagatcgg
     1561 cctgaccgtg cccacgatct gggagcaggt ccgcactttg gaggcccgct accgcgcggc
     1621 cctctttcgc cgccgcggcc ggcggattga ggccacgccc accgccgcat tactctacga
     1681 ctcgctgcgg ccgctcctgg cggggctgga ttccaccttc gccctggtca gcgaagccgc
     1741 gggcgatcat ccccgcacgt tgactttggt caccggggcg cgggccatgt tcgaagacct
     1801 gaccgcgccg ctgcgcgaat tccgcgcccg tcaccggcaa gtggctttgc ggctgctcga
     1861 ttctggcacc cggcgggccg ccgagctggt cgcgaccgac aaggccgact tggcgctcgc
     1921 gctggagcct gggcccggca tggtcggccg cgagatctgc gtcgagcggg cctaccgcat
     1981 cgaatacctg gcgatctttc cacggcgtca tccgctggcg aagtcggcca ggctgcggct
     2041 ggccgacctg gtggctcatc cgctggtcgt gggccacgac ggcacctacg gccgccagct
     2101 cctcgaccag gccctgcacc gcgagcggct gacggagcag gtgcagatcg tcgccgagac
     2161 cgacaacagc gccttcacga ccgcctgcgt gcgggccggg ctgggcgtgg gcgtcgtggc
     2221 ggggcggccg gccggggcgc tctgccgcgg gctggcctcg cggtcgctcg cgccgcacct
     2281 gggcgaagcc tggatcgcct ttctctggaa gcgcggccgc cggctgaccg ccacgctgca
     2341 gacgctcatg cagttgatcc gcgagcaggc cggcgtgtag cgtgcccgct gctttcgcgt
     2401 tcgcgatcca gcaacgctcg ctttcgcgac aggccccagc ggtagccgcc gaggccgcca
     2461 tccgctcgaa ccgcccggtg gcaaggcacc gctacggcga cgggattcgc cgcacaggcg
     2521 ctgccaaccg cccgcacggc tttcggccgg cggatactgg cggcgatctg cgaataggtg
     2581 gcggtcgtgc ccgggggtat ggcccgcagg gcatcccaga cacgacgctg aaaggcggtg
     2641 ccctgaatgt ccagcggcaa gtcgcccgcc tcgctcggcg tctcaatgcg gctcactacc
     2701 tgccgcaccc aattctcgaa ttcggcatcg cccagagcga cgctggcctg ggcaaactgc
     2761 cggcgggcgg acctcaagag ctcgtcggca tcggcgccca actggacgag gcaaactccg
     2821 cgggccgtgg cggcgacgac gacgcagccg aggctcgtct cggccgtggc caggcgaatc
     2881 gtctcgccga gggcctggtg gcgaaacgct gccggccgca tgcccagcga tttcccggcc
     2941 tcgtcgtaga accggctgct ggcccgataa ccggcggcat aggccgcctg cgtgaccggc
     3001 atgccgctag ccagggcgat acgcgcccgg tccgtccgcc gcgccgcggc atacactttg
     3061 ggcgtgaccc ccgtcacgcg ccgaaacagg cggtgaaact gcgaagcgct gtaacccgac
     3121 gctgcagcaa cctcggcgag cgaattcgtc ccgtgttcat tgtcgagcat gtggcaggcg
     3181 cgagcaatca gcttcgccaa ctcgcctgca gggtttcgga ggtcagggcg gcaacgcttg
     3241 caggcccgaa atccggcggc ccgtgcgtct cccgcggacg gaaagaacaa cacgttctcc
     3301 cggttgggca gccgcgagga gcagccagga cggcaaaaga cgccggtcgt ccgcacgccg
     3361 aaatagaacg cggcggacgc ctcgcgccgc tcgacgattc gccacatcgc gcggggcgac
     3421 agcgcgggtt tcggcggccg cggggtcgtc ttcaacatgc ctggtactcc tgaggagcga
     3481 tacaccatca tgccgcttcc gccgccgtgc gacttcccga aaagttccag gcaattcggt
     3541 ttctgggctc gatgcgcggg tggccgcgtc cgcgcaatcg tatgcgtagt ggacattggt
     3601 atgaattttg cactatggtt cctgttgcgc tgccggctgc caatttggca ttggggccga
     3661 agacccaggc ccgacctgag aggcgccagt aggtggtctc aaacacttat ttaatagtat
     3721 ttaagggggt tgcgcattat cgatcgcaag catatgcgcc gtctcacctt gcggcgaaat
     3781 tggccgaaaa atcgccgttc gaggtgacgc tttcgggtgg tcccgaaggg cccgccataa
     3841 caaaggccgt cagggaacca tcaccaccga gccggtcgtc tggcggcctt ccaggtcgcg
     3901 atggacctgc tgggcgtccg ccaggcggta ggtgcggtgg acttcgatct tgaccttgcc
     3961 ggaaaggacc acgtcgaaca aatcccgcgc ggtggcttcc aggtcggacc gcgtggcggt
     4021 gtaggtcgcc agcgtcggcc gcgtgacata gagcgagccc ttgggagcga gaatgcccag
     4081 ctcaaacggc ggtacggccc cagaggcatt gccgaagctt accaagagac caaacggccg
     4141 caggcaatcg agcgagccgg cgaacgtgct cttgccgacc gagtcgtaaa ccacgggcac
     4201 ccgctggccg ccggtgatct cgcggacccg ctggacgaag tcctcgcgcg tgtagtcgat
     4261 cgcgtgcgtg cagccgtgag ccttggcgat ctccgccttt tccgcggagc cgacggtgcc
     4321 gatcacggtg acgccgagag ccttgagcca ctggcaggcg atcagcccga cgccgccagc
     4381 ggtcgcgtgc cacagcaccg tctcgcccgc ctgcacctta taggtgcggc ggatgaggta
     4441 ctggaccgtc atgcccttga gcatcatcgc ggccgcttgc tggtcggaga tgcccgcggg
     4501 cagcttgagc agtcgatcgg ccggcatcag gcggacttcg gagtaggagc ccggcggacc
     4561 gccggcgtag gccacgcggt cgccggcctg gatgtgggcg acgcccttgc cgacggcctc
     4621 gacgacgccc gccgcctcgc tgcccaagcc gctgggcaag ggcagcggat aaaggcccga
     4681 gcggtggtac gtgtcgatga agttgacgcc gatggccgtg tgccggacgc gggcctggcc
     4741 ttcgcccggc tcgccggcgg cgacgtcttc aacacgcaga acttctggtc cgccggtctg
     4801 cgagaagcga atggctttgg gcatggcaga tatcccgtgc gagtcggctc aggataacgt
     4861 gcctgctggc tgtcggccat cgcgtgcgct aggcagcacg gcgcgcgagc tttttcaggc
     4921 gtcggcgctc ggcgcgcgaa agcctttcgc cgccatagtc gctggcgtcc tcctcgtcgt
     4981 cgtcttcgtt gtcgtagtgc gagggcgact tcgaaatggt agcgccggac ttgggcgcac
     5041 cggaggccgg cgcgccgaac ctggcgggct cggctggccg ggacggcgcc gccgcggcgg
     5101 tcttctgggc gggcgggctg tcctcgactc gatcctcctt gacgaccttc agcttcgcac
     5161 ggccttttcc cttcgcggct cgcttcttgt cgggatcgat gtggaccctc aggcgcccgg
     5221 tcgcgtcgag atagacgtgg cgggcataga gaccgatcga cgtggtgagc gccaggtgcg
     5281 ccagcagcgt cagcgtcgat tgcaacaccg actggcccag cggcgtggcc gcggcgagca
     5341 tgtcgagctg gagcaaagcg gcgcagagat agagcagtgc ggccaccagc agcgccgcaa
     5401 agctcggcag cgacggccag acttcgatcg ccaggcggat gccgagcgtg ccgagcacga
     5461 ggccgtacag ggccagccac atggcggtgc tgcccgcggc gagcgaagag ccgagcccgt
     5521 gctggccggc ggccgcgttg acggccaggc ccagggcgtc gtgcacgcct gtcgcggcgt
     5581 ccaggctcag ccaggccagc gccgccgtcg tccagatcca gatgcgatag ctgccgcggt
     5641 agtcatcgac gcggtgcacg cggatcgcga acacgacgag ggccgctgcc gtggccgttg
     5701 ccagcagcag cgacgaatac caggccgcca ggctgccgcg cgaagaagcg tcgagcgccg
     5761 ccagctgctc gcctccgggg gccagcggca gcgcggccgc gtgaatgtga atggcctcga
     5821 tcgcggcgat ccccgtcagg cccagcagca tcgcgaccgc cacccacagc ggccgcacgg
     5881 gcaacaggtc cgtcacgcgc ggctgccggt cgctgagcac ggccgggcta taggcgcgga
     5941 cggggccctt cggcgaaccg ggcgcgggcg ccgggtccga atcgtcggag gggggcgagg
     6001 cgatctcatc catgagaacg cgccgccgcc gctcgtcgag ctggccgctg cgtcgaaaat
     6061 ccattttctt cgcgggctga cgcgggaggg gaaggcgctc gtgacagcgc cttgttggtt
     6121 ccattcggct gccagcagcg gcaaactgca ctcgcggcgg cggcgccgac aaggctggga
     6181 gcagccccaa gagccgccag cgagtttgcg ggctggtgaa tttgtggcga ggtcgattct
     6241 ggcagctctc gctggcgctg gctttgccag agcgaattgg ctagatttgc aattagggcg
     6301 aagcagaggg caagcaatca aaattggcga ggaaattcag atggtacgca tacggcgaat
     6361 tcggacttgg ggagcgttgg cggcgggggt cgcgatcgga ctggccatcg gcggggcgct
     6421 cgcggccggc gtcaccttcg ggcggtggaa ttcgcagacc gccggcatcc cgggagcgag
     6481 cgacttgcgc ttgaaggcca tggcggccta tggcaacgac aactttgcgg ttgcgaccgg
     6541 cgtgatcgac aacgacgtcg agggcctgta cacgctggac ttcctcaccg gcgacttgca
     6601 gtgctacgtc gtcaacccgc gcaacggcgc gctgggcggc tggttcaaga ccaacgtcgc
     6661 caaggacctg ccgccggaag gcaccaagaa gcccagctac ctgctcgtca cgggcacttt
     6721 tcgggtgcaa ggcgccgccg gcaatcaaaa gccggcggcc agcttgtgct atgtggtcga
     6781 tggcaacacg ggcaattgcg tggcctattc cttcccgtgg ggccagtcgt attcggcgtc
     6841 gggacaggtc cagtccacgc cgatgcaagc ggtcggcaag tggaagaccc gcaccgtggg
     6901 cgttcgcgag tagcacccgc ggggcgctcg catgaccctg aacctcagcg actacgtcgt
     6961 cgccggcgag atcatcaatc gccggccgaa tatgacgcac ggctggatcg cgctgcgagg
     7021 ccaggaccag ccgctgctgg tgcagctcac cggcaatccc tcgccggatc tggtcggcaa
     7081 gcgatttcgc tttcagattc ccgatgagcg gcccccgccc gatccgccgc cgcccccgct
     7141 cgacgcgaag cagatcgcct ggcaacagat cggacccacg gggccgatgt cggtcgagac
     7201 gactgccgag ggaaagccgc gcgtgcggct ccagtggcac agccagaacg gccacgtgct
     7261 gatcgaggtg aacgatccag agctggagtg ggtcgaggac gacgaggcgg acgccgctcg
     7321 gcagaaggag ctcgaagcgg ccgaggcggg cgagcgcggg gaggatgccg aagactcggc
     7381 ggcgctcgat ctcggcgtgg ggctgccgga cgaagatgag gacgatcctt acggcctctt
     7441 tccagaaggg ctcgacgagc agctcgccgg cgaggaggaa acggacgaac cggccaaacg
     7501 gcgcgagccg cgggactgga gcgaggtgat ccccggcatc gacgatgaaa cgaagaagat
     7561 gtacgaggag tgggacgagg tgaccgaggg cacgaaggac gtgccgctca cggaagtctt
     7621 cgacccgccg atcaagattt atccggccga gcagctggcc gcgctcgatg acgcgacagc
     7681 ggaagccacc ctcaaggagc tgctccagcg gctggcgctg cacggcgtcg cgctggccat
     7741 ctgcgagcac tttacgccgc gaatggccta tcgctacctg acggaggaga tcctgcccga
     7801 atacggcgtg catccccgcc tgccgcaaat cggctgggtg cagcactacg acacctccga
     7861 ggactgcccc gagtgtcagg cccgtatcga ccgcgaatgg gaagagcgga agggcaattc
     7921 gggcggcgag ccggatgaag cctgagtcgc aaccgcaagg gtgctaacgg tttgcggcgc
     7981 gtttcaggtc tctggggatc aaaacccgac cggccgtcgt ctataattaa gcgatcaggg
     8041 tgcgcgccgg ctcgcgagga gatcatggca agcggaactg tcccttcggt tgtaccgcct
     8101 cccatcgcgg agagcctgca gcgcgagcag gtcatccagc ggcagctcgg acgcaccagc
     8161 ctgcacgtgc ggctgatcga cctggcaagc ggtctggcct tctggctggt gggcgtggtc
     8221 gtcttcttca tcgccgcggc cgcattcgac catttgatcg gtttggggac catcggccgc
     8281 gtgttgggac tcgtggcgct ggtcggcggc tcgtcctggt acctggtcgc cgtcatctgg
     8341 ccgctcgtcg cccggagcat caacccggaa tacgccgccc gcacgatcga agaggcgacg
     8401 ccctcgctga agaacagcct gatcaatttc ctgatgctcc gcagcgagcg cgggaagatg
     8461 aaggagatcg tgtttcaggc gctggagcag aaagcggcca gcgacattgc cggggtgccg
     8521 gtcgaatcga ccgt
//
LOCUS       DASRJB010000713         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1332642, whole genome shotgun sequence.
ACCESSION   DASRJB010000713 DASRJB010000000
VERSION     DASRJB010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332642"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFB80_18880"
     CDS             complement(<1..392)
                     /locus_tag="VFB80_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35905.1"
                     /translation="MMLLIDLTSLFLAACGGAAAMWVVMWFRAPLPEPLDEDEAGFAR
                     DTISKLQDLTRRVKDEVDQHAVCVEEISAQLTNTDDNDESAVLAAVAQLIDANQQMQR
                     KLDTAEERLKTQARQIESHAVEARTDALT"
     gene            complement(583..1485)
                     /locus_tag="VFB80_18885"
     CDS             complement(583..1485)
                     /locus_tag="VFB80_18885"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35906.1"
                     /translation="MESDTPPPLRYTAAMLAELAGVDVARVRAWQRRGWIVPASETHR
                     LAHFDFTELTVARQLAQLTRAGATPRLLARKLAEIHRHWPDVGRPLAELTLVLDGKKL
                     LVRRGTELLEPGGQLRIDFEALAEDSQPDPPATILSSSLFLARQSESPVATPEQFAAW
                     AAEMDEAGDLKLAAELYRTALAAGGPKPELCFQLAEALYRAGDLPAARERYFMAIELD
                     EDYVEARANLGCVLLDLGEKELAAAALEGALHSYEPYADVHYHLARTLDELGRLDQAA
                     PHWSRFLELAPDSPWADEARERLP"
     gene            complement(1563..2159)
                     /locus_tag="VFB80_18890"
     CDS             complement(1563..2159)
                     /locus_tag="VFB80_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35907.1"
                     /translation="MTITTGVTAHHCPECGAELRPGDGRCWLCRRDLAVDAEVVDPAP
                     AAAAAKAAPLQFSLETLLLIVTLSAVCLGALVAAPGLGVLLLVVAVPALVRTCLTGSS
                     FKQKSGKLTAADKVLAFVASAAITWAALMAAAMAFFTACTISVLGACAVGSAGGSSAI
                     DQSLQTVLLWIAIIFCAATSIAAFVGMFWVTWPRATRR"
     gene            complement(2224..3579)
                     /locus_tag="VFB80_18895"
     CDS             complement(2224..3579)
                     /locus_tag="VFB80_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN35908.1"
                     /translation="MTASRQCRRRFLAQTAAVGVAALGAPALLRAQNTTSKLNIAFIG
                     VGGRGRANLNEIARTGAENVVALCDVNGNNLEGAAQRFPSARKYRDFRKLYDESKDID
                     AVVVSTCEHTHAYATLPALQMGKPVYCEKPLTLNVAEARIITEAAKKAGVPTQMGTQI
                     HASSNYHRVVELIQSGAIGPVSEAHVWVARAWGRQSKEDSDANKDIVFVTERPKEEQQ
                     PPSYLDWDLWIGPAPYRPYHEVYFPGPKWYRWWDFGNGTMSDLGSHWNDLPFWALDLD
                     APTSIEASGPEPHPEIAPASMSAIYEYPARGSRPACKLTWYQGTHKPQLWKENPAIQR
                     FGSGVLFVGTNGRMLVSDYGKHVLLPEAEFKDFKRPEPFIPDSPGQQAEWLRAIRENK
                     PTGSPFSYAGLLTEANHLGNVAYRAGTKILWDSASMRVTNTRAADPFLSRQPRAGWKL
                     A"
     gene            3751..>3935
                     /locus_tag="VFB80_18900"
     CDS             3751..>3935
                     /locus_tag="VFB80_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZN35909.1"
                     /translation="MRTHPHQPAWREELIDTIHQHRLSRRRFLSRAAAIAAGGLVGGL
                     AQIDAFAQQAAADNASKA"
BASE COUNT          635 a         1298 c         1331 g          671 t
ORIGIN      
        1 gtcagggcgt cggtgcgggc ctcgacggcg tgcgactcga tctgccgggc ctgcgtcttc
       61 agccgctcct cggccgtgtc gagcttccgc tgcatctgct ggttggcgtc gatcagctgg
      121 gcgacggcgg ccagcacggc ggattcgtcg ttgtcgtcgg tgttggtgag ctgggcgctg
      181 atctcctcga cgcagaccgc gtgctgatcg acttcgtcct tcacccggcg ggtcaggtcc
      241 tgcagcttcg aaatggtgtc gcgggcaaag ccggcctcgt cttcgtcgag cggctccggc
      301 aaaggcgcgc ggaaccacat caccacccac atggcggctg ccccgccgca agcggccaga
      361 aagagcgacg tcagatcgat gagcagcatc atggacaaac ggcggggaac gcgagaggtg
      421 ccccaggtcc acagcatctc ccagtgtgac ttagtttttt caggccgcgg gtcaagccga
      481 aacttgttgc cggggctgga gatacgcaaa atccccgcgc ttcaattccc gtcgccacga
      541 gggcggcaaa tcgccgcatg gaatgtgccg gctgtagcgg ttttagggca gccgctcgcg
      601 ggcttcgtcg gcccaggggc tgtcgggagc gagctccagg aaccgcgacc aatgaggggc
      661 ggcctgatcc aagcggccga gctcgtccaa ggtgcgtgcg aggtggtaat gcacgtcggc
      721 gtacggttcg tacgaatgca gcgctccctc cagcgccgcg gcagcgagct ccttctcgcc
      781 caggtcgagg agcacgcagc cgaggttggc tcgggcctcg acatagtctt catccagctc
      841 gatcgccatg aagtaccgct cgcgagccgc cggcaggtcg cccgcgcggt agagcgcctc
      901 ggcgagctga aagcagagct ctggtttcgg gccgccggcg gccagggccg tgcgatagag
      961 ctcggcggcg agcttcaggt cgcccgcttc gtccatctca gccgcccagg cggcgaattg
     1021 ctcgggagtc gcgacgggcg actccgactg ccgcgcgaga aacagcgacg acgacaggat
     1081 ggtggctggc ggatcgggct ggctatcctc cgcgagggct tcgaagtcaa tccgcaattg
     1141 cccgcccggc tcgagcagct ccgtgccgcg gcggacgagc agctttttac cgtcgaggac
     1201 cagcgtcagc tcggcgagcg ggcggccgac gtctggccag tggcggtgga tttcggccag
     1261 cttgcgcgcg agcagccgcg gcgtcgcgcc ggcccgcgtg agctgcgcca attgccgggc
     1321 gacagtcagc tccgtgaagt cgaagtgggc caggcggtgg gtctcgctgg ccggcacgat
     1381 ccacccccgc cgctgccagg ctcgcacccg cgcgacatcg acgcccgcca gctcggcgag
     1441 catcgccgcg gtgtagcgca ggggtggagg cgtatcggat tccatcgacg aaccgccgtc
     1501 aactttgagc cgtcaacttt gtaccgggga accttgaact ttgaacttcg ccgcggcggc
     1561 cgtcaacggc gcgtcgcgcg tggccaggtg acccagaaca tcccgacaaa ggcggcaatg
     1621 ctggtcgcgg cgcagaaaat gatcgcgatc cacagcagga cggtttgcag cgactggtcg
     1681 atagcggagc tcccgcccgc acttccgacg gcgcacgctc ccagcacgga gatcgtgcag
     1741 gcggtgaaaa aagccatcgc ggccgccatc aacgcggccc aagtgatggc cgcggacgcg
     1801 acgaacgcca gcaccttgtc cgccgccgtc aacttgccgc tcttctgctt gaaagacgat
     1861 ccggtcaagc aagtgcgcac gagcgccggg acggccacga ccagcagcag cacccccagc
     1921 cccggcgcag cgaccagcgc ccccaggcac accgcggaga gcgtcacgat caagagcagc
     1981 gtttcgagcg agaactggag cggcgcagcc ttggccgcgg ctgccgccgg agcaggatca
     2041 acaacctccg catcgaccgc caggtcgcgg cggcagagcc agcagcgccc gtcgcccggc
     2101 cgcagctcgg cgccgcactc ggggcagtgg tgggcggtga cgccggttgt gatcgtcatg
     2161 atcgactccg tcctgagtac tcagtaccag gtaccaagta cgactccgac cgcatggcga
     2221 cacttacgcc agcttccatc ccgctcgcgg ctggcggctg aggaatggat cggcggcgcg
     2281 ggtattggtg acgcgcatgc tggcgctgtc ccacaggatc ttcgtgccgg cgcgataggc
     2341 gacgttgccc aggtggttcg cttccgtgag caggccggcg tagctgaatg gactgccggt
     2401 cggtttgttc tcgcgaatcg cgcggagcca ctcggcctgc tggcccggcg agtcgggaat
     2461 gaacggctcg ggcctcttga aatccttgaa ttcggcctcg ggcagcagca cgtgcttgcc
     2521 gtagtcggac accagcatgc ggccattggt gccgacgaac agcacgccgc tgccgaaccg
     2581 ctggatcgcc gggttttcct tccagagctg cggcttgtgc gtcccctggt accaggtcag
     2641 cttgcaggct gggcggctgc cgcgggccgg atactcgtag atcgccgaca tcgaggccgg
     2701 tgcgatctcc gggtgcggct cgggtccgct tgcttcgatc gacgtcgggg cgtcgaggtc
     2761 cagcgcccag aagggcaagt cgttccagtg gctgcccagg tcggacatcg tgccgttgcc
     2821 gaaatcccac cagcggtacc attttggccc ggggaaatag acctcgtgat agggccggta
     2881 cggcgcggga ccgatccaca ggtcccagtc gaggtagctc ggcggctgct gctcttcctt
     2941 cggccgctcg gtgacgaaga cgatgtcttt gttggcgtcg gagtcttcct tgctctgccg
     3001 gccccaggcc cgggcgaccc agacgtgggc ttcgctgacg gggccgatcg cgccggactg
     3061 aatcagctcg acgacgcgat ggtagttgct gctggcgtga atctgagtcc ccatttgcgt
     3121 cggcacgcca gccttctttg ccgcttcggt aatgatgcgg gcctcggcca cgttcagcgt
     3181 cagcggcttc tcgcaataga ccggcttgcc catctgcagg gccggcagcg tcgcataggc
     3241 atgcgtgtgc tcgcaggtgc tgacaaccac cgcatcgata tccttcgact cgtcgtacag
     3301 ctttcgaaaa tcgcgatact tgcgggcgct tggaaaccgc tgagccgccc cttcgaggtt
     3361 gttgccgttt acgtcgcaca gagccacgac gttctccgct ccggtgcgag cgatctcgtt
     3421 caggttcgcc cgcccgcggc cgccgacgcc gatgaaggca atgttgagct tgctggttgt
     3481 gttctgggcc cgcaacagcg cgggcgcgcc gagcgccgcg acgccgacgg cggcagtttg
     3541 agcgaggaac cggcggcgac attggcggga agcggtcatg gaatgactcc cggaggtgga
     3601 tgacgcgcac agcatgacgc gccgcgccgc gggctgcaac ttgattctgc cactggctcg
     3661 ctggcaacct gtttttcggc gtgctactgt ggccgcgcgg ccccttttca cggcgttctc
     3721 cttcccgtca attttgcgaa aggctcaccg atgcgcaccc atccccatca gccggcatgg
     3781 cgagaagaat tgatcgacac gattcaccaa catcgccttt cgcggcgccg atttctcagc
     3841 cgcgcggcgg cgatcgccgc gggtggcctg gtcggcgggc tggcgcagat cgatgccttc
     3901 gcccagcagg cggcggccga caatgccagc aaggc
//
LOCUS       DASRJB010000714         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1058218, whole genome shotgun sequence.
ACCESSION   DASRJB010000714 DASRJB010000000
VERSION     DASRJB010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058218"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFB80_18905"
     CDS             complement(<1..1150)
                     /locus_tag="VFB80_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZN35910.1"
                     /translation="MTERRVTQLLPTAALAVVGVALGGAWVVEPSLAPSGWLGRLLVL
                     ASAIGLALFAVKIAMRQSAKTAEAARKYIDTLCRLEGPELHDEAAFDTIPLRKGDRIW
                     RELCQAIRARLVEFSARAEELEMARAAAEVRVRRTAIERDQVREILAGLADPVVAVDQ
                     FGDVVLANPSAERLLDIRPAGDAHPALEQLDRCEQLVSMLGETRRRRSATQRSAEVTL
                     SDEEGKPHWYRVSCRTLASQHESCGGADRTAQHGAVAVLTDITGQKAIQKRNAEFVSA
                     VSHEMKTPLSSIRAYVELLVDGEAEDDATREEFLGVINNQADRLQRLIDNLLNLARIE
                     AGVVAVNKSPQSLNERLAEAIRVLEPTAEQKQIKLTTEFSELYLGVLGD"
     gene            complement(1367..1855)
                     /locus_tag="VFB80_18910"
     CDS             complement(1367..1855)
                     /locus_tag="VFB80_18910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35911.1"
                     /translation="MNGCLRLALLASILFGGCAAWRGQRPATDVAVERQQRAADAVRT
                     FEEQRDGAQFQAALDRWAAGDIAGCEGRLRALAARRPADGAVRLRLAELLWSRGAPGE
                     AEAELQAVLAVLPASAEAHHMLGMILSEQARLAEARSHLSRAIELAPQCDDYRATLAS
                     LP"
     gene            complement(1852..>2167)
                     /locus_tag="VFB80_18915"
     CDS             complement(1852..>2167)
                     /locus_tag="VFB80_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35912.1"
                     /translation="LRDQIVAAGGFENESIHEYLHRGLAPLTGRHIDYSRRGFPWKDP
                     PEFGGPLAVSASDDLGQIGPTRDILVPPPPGGVLRLPPEPLPAQPLPPPRLSPPPGGP
                     ER"
BASE COUNT          330 a          759 c          738 g          340 t
ORIGIN      
        1 gatcgcccag cacgcccagg tacaactcgc tgaactccgt ggtgagcttg atctgctttt
       61 gctcggcggt tggctcgagc acgcggatcg cctcggccag gcgctcgtta agcgactggg
      121 gtgacttgtt caccgccacc acgcccgcct cgattcgggc caggttgagc aggttgtcga
      181 tcagccgctg gagccggtcg gcctgattgt tgatgacgcc caggaactcc tcgcgcgtgg
      241 cgtcgtcctc ggcttcgcca tcgacgagca gctcgacgta cgcccggatg ctcgacaggg
      301 gcgtcttcat ttcgtggctg accgccgaga cgaactcggc atttcgcttc tggatcgcct
      361 tttggccggt gatgtcggtg agcaccgcga ccgcgccgtg ctgagctgtc cggtcggcgc
      421 cgccgcagga ttcatgctgg ctggccaggg tgcggcagga aacgcggtac cagtgcggtt
      481 tgccttcctc gtcgctcagc gtcacttcgg cgctgcgctg cgtcgccgag cggcggcggc
      541 gggtctcgcc gagcatgctc accagctgct cgcatcgatc tagctgctcc agggccgggt
      601 gcgcgtcgcc cgcggggcga atatccagca gccgctcggc gctggggtta gccagcacga
      661 cgtcgccgaa ttgatcgacg gcgacgaccg gatcggccag gccggccaga atttcgcgga
      721 cctggtcgcg ctcgatggca gtgcggcgaa cgcggacttc cgccgcggcg cgggccatct
      781 ccagctcttc cgcccgagcc gagaattcta ccaaccgggc acgtatcgcc tggcacagct
      841 cccgccagat gcgatctccc ttgcggagcg gaatcgtatc gaaggcggcc tcgtcgtgca
      901 gctccgggcc ttccaggcgg cagagcgtgt cgatgtactt gcgggcggcc tcggccgtct
      961 tggccgattg ccgcatggcg attttcactg cgaacagggc caggccaatt gccgaggcca
     1021 gcaccagcaa gcggcccagc caaccgctgg gcgcgagcga cggctccacg acccaggcgc
     1081 cgcctagggc cacgccgacg accgccaagg cggccgtggg gagcagctgc gttacacgtc
     1141 gctcggtcat gcctgcgtct ttcgatcgga gccccaaccg gcgcggctac ccgcgcacca
     1201 ccagcgcgca agcgcctggc agggacgcca atccacgcaa acctaggtta gccatacact
     1261 ttgtccacgc tccaaccatg gcccgctctg gggatgcggt taaccccatg gctgtgcggc
     1321 ggcataggcc gaacgaccgc tagcggcgat aggcccgcga aatccgctag ggcaaagagg
     1381 ccaacgtggc ccgatagtca tcgcattggg gcgccagctc gatcgcccgc gacagatggc
     1441 tgcgagcttc ggcaaggcgg gcctgttcgg agaggatcat ccccagcata tggtgggcct
     1501 cggcgctcgc cggcagcaca gccagcacgg cttgcagctc cgcctccgcc tcgccaggcg
     1561 ccccgcgcga ccacagcagc tcggctagtc gcagacgcac tgcgccatcc gcgggacgac
     1621 gagccgccag ggccctcagt cgcccttcgc agcccgcgat gtcgcccgcg gcccagcggt
     1681 cgagtgcggc ttgaaactgg gcgccgtcac gctgctcctc gaacgttcgc accgcgtcgg
     1741 ccgcgcgctg ctgccgctcg actgccacgt cggtagccgg ccgctggccg cgccaggcgg
     1801 cacagccgcc gaacagaatg ctcgccaaca acgccagccg caggcagcca ttcatcgttc
     1861 gggccctccc gggggcgggc tcagtcgcgg cggaggtagc ggctgtgcgg gcagcggctc
     1921 cggcggcagg cgcagtacgc cgcccggcgg cggaggcaca aggatgtcgc gggtggggcc
     1981 gatttgtccg agatcgtcgc tggccgaaac cgccagcggc ccgccgaact ccggcggatc
     2041 cttccaggga aaaccgcggc gggagtagtc gatgtgccgg ccggtgagcg gtgccaggcc
     2101 acgatggagg tattcgtgaa tgctctcgtt ctcgaagccg cccgcggcga cgatctggtc
     2161 gcggagg
//
LOCUS       DASRJB010000715         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3592363, whole genome shotgun sequence.
ACCESSION   DASRJB010000715 DASRJB010000000
VERSION     DASRJB010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592363"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="VFB80_18920"
     CDS             <1..1798
                     /locus_tag="VFB80_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35913.1"
                     /translation="VAKTPADRLSLLLVEEYFDAEDERFLAALRQQYPPAALAAFADR
                     WKKDTRPWARRQIFLYLSMPLDVPAHHPVVKRLFKHAEQTGDHELMGAFLHTFDCLVR
                     KYRKKLKRWDWDPQTRRSSATEEEVLKLPTNVLAPWKVQEYRNPRTGQKIEYPDHRAQ
                     ARRGAVLFSYATRRYLRRRAWRYFRHLAHQDPLKYLVAITLALGRYEDKELAHGEDLL
                     ESWGLMHACFHGHDALEFKPIGVKLRPGRSLSELSPAPYRPELWKTEVAFPVLLGAMI
                     GDGSRCIRTWCRQLLEREHATRLQSISAELLLMLLDSSDEEVQQFGARLLGSLDSAGH
                     WPVETWLRLLETKDPAAIALICEAMQKHVRGDRLDLAQCLAMATARATPVAKLGLTLL
                     QQRKFAAAERGQLTLIGNARCAAIGAELATWTLGIVGAAAAYDRDVVLALFDSLLKEV
                     RTAAWDWLRAGPAIPADPQLWCRLLETPYDDLKLALVDHLQSRSALPGTSTSDLAPIW
                     SAVLAGVHRGGRQKLKAIDQLAAAVCRDPEQAGDLVPVLALAVRSIRKPEARAALAAV
                     ARIVGSRPELEPLVAAKLPELSLTAPAALPTV"
     gene            1810..>3467
                     /locus_tag="VFB80_18925"
     CDS             1810..>3467
                     /locus_tag="VFB80_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HZN35914.1"
                     /translation="MQVSFAYHGRSRLEGGLLSFAPNLSREAVAFDAPLVRPLRFREG
                     ISALHDVVISDLRYKPRDHAAYEEWKKGEQVRRREQLQQFREVATQEILARRGIPAPP
                     DLERQFEVCRKRYWSARLDYSSYLQKNDQELWRLLVPCDPIVTVADDVVFFECFSADE
                     SSYGCLTVERGAFGPAGGVQLGTTNVDYSWDLYHHFQALRTYRETRLKVDPAGFEVAT
                     QGSSDYREEKIDLPAGWLRGLAQVQAAMTLPMRRVSLTREAVYSLLAWLKRHKAQKSP
                     RALRFELWPDCPPRLVLEPWETAVYSYGTTYSGPPGEPIRIWGRQRLLVLARLLPLAD
                     QFDVYLLGTGLPSFWVARMGEMRLTVGLSGWTTNDWTRNAALDVVRPPVKLSEGLTSG
                     VAQAMRTHRTLAPAATALKSVASEAECAAALNHLAHAGQVIYDLAAGVYRWRQIMPRA
                     LGEAEIGPENAELAASRGLVAKVKIDSRQEAPRGGTVYTAVIDRMQIEMLLDADGIIK
                     RGKCKCSHHFKGGLRMGPCRHLLAVRAAVLGGTQQAGDLQAWFAR"
BASE COUNT          553 a         1159 c         1207 g          548 t
ORIGIN      
        1 tgtggccaag actcccgcag atcggctgtc gctgctgctc gtcgaagaat acttcgacgc
       61 cgaggacgag cgtttcttgg cggccctcag acagcaatat cctccggcgg cgctggcggc
      121 gtttgccgac cgctggaaga aggacacgcg gccctgggct cggcggcaga tcttcctcta
      181 cctgtcgatg ccgctggatg tgccggcgca tcacccggtg gtcaagcggc tgttcaagca
      241 cgcggaacag accggcgacc acgagctgat gggggcgttc ctgcacacgt tcgactgcct
      301 ggtgcggaag tatcgcaaga agctcaagcg gtgggactgg gatccgcaga cgcggcgcag
      361 cagtgcgacg gaagaggagg tcctgaagct ccctacgaac gtgctcgcgc cgtggaaagt
      421 gcaggagtac cgcaatccgc ggactgggca gaaaatcgag tatccggacc accgcgccca
      481 agcgcggcgc ggggcagtgt tattttccta tgccacgcgg cggtatctgc gacgacgggc
      541 ttggcgctat ttccggcacc tggcgcacca ggacccgctg aaatacctcg tcgcgattac
      601 gctcgccctc ggtcgctacg aggacaaaga gctggcccac ggtgaggacc tgctggagag
      661 ctggggcctg atgcacgcct gctttcacgg gcacgacgcg ctggagttca agccgatcgg
      721 agtcaagctc cggcctggcc ggagcctgag cgagctgtcg cctgcgcctt accggcccga
      781 gttatggaaa acggaagttg cgttcccggt gctgttgggt gcgatgattg gcgacggctc
      841 gcgttgcatt cgcacctggt gccggcaact actggagcgc gagcacgcca cacggctcca
      901 gtcgatttcg gccgaattgc tactgatgct gctcgatagc agcgacgagg aagtccagca
      961 gttcggcgcg aggctgctcg gttcactcga ttccgctggc cactggccag tcgagacgtg
     1021 gctgcggctg ctggagacta aggaccccgc ggcgattgcc ctcatctgcg aggcgatgca
     1081 gaagcacgtc cgcggcgacc ggctggacct ggcccagtgc ctggcgatgg ccacggcccg
     1141 cgcgacgccg gtcgccaagc tcggcctgac attgctccag cagcgcaagt tcgccgccgc
     1201 cgagcgcggc cagctgacgc tgatcggcaa cgctcgctgc gcggcaatcg gcgcggagct
     1261 ggcgacttgg acgctgggca tcgtcggtgc agcggccgcg tacgaccgcg acgtcgtgct
     1321 cgcgctcttt gattcgctgc tgaaggaagt ccgcaccgcc gcctgggact ggctccgcgc
     1381 cggcccagcg attccggccg acccgcagct ctggtgccgc ctgctggaga cgccctacga
     1441 cgacctgaag ctggcgctgg tcgaccattt gcagagcaga tcggcgctgc cgggcacgag
     1501 caccagcgac ctcgcgccga tctggtcggc ggtgctggcc ggcgtccacc gcggcgggcg
     1561 gcagaagctg aaggcgatcg atcagctggc agcagccgtc tgccgcgatc cagagcaagc
     1621 cggcgacctc gtgccggtgc tggcgctggc cgtccgctcg atccgcaagc ccgaagcccg
     1681 cgcggcgcta gccgccgtcg cccgcatcgt cggcagccgg ccggaactgg agccgctcgt
     1741 cgcggcgaag ctgcctgagc tgtcgttgac cgcccccgct gccttgccga ccgtctgaag
     1801 gaacgccgcg tgcaagtgag ctttgcctat cacggacgca gccggctcga agggggcttg
     1861 ctctcgttcg cgccgaactt gagccgcgag gcagtggcgt tcgacgcgcc gctggtgcgg
     1921 ccgctgcggt tccgcgaggg catctccgcc ctgcacgacg tggtgatcag cgacctgcgc
     1981 tataagcccc gcgaccacgc ggcgtacgaa gagtggaaga aaggcgagca agtccggcgg
     2041 cgcgagcagc tccagcaatt ccgcgaagtg gccacgcagg aaatcctcgc ccgccgcggc
     2101 attcccgccc cgcccgacct ggagcggcaa tttgaggtct gccgcaagag gtactggtcg
     2161 gcgcggctcg attactcgag ctacctccag aagaacgacc aggaattgtg gcggctgctg
     2221 gtgccctgcg atccgatcgt cacggtcgcc gacgacgtcg tcttcttcga gtgcttctcg
     2281 gccgacgagt cgagttacgg ctgcctgacg gtcgagcgcg gcgccttcgg cccggcgggc
     2341 ggcgtgcagc tcggcacgac caacgtcgat tactcctggg acctctacca ccatttccag
     2401 gccctgcgga cctatcgcga aacgcgactg aaggtcgatc cggccggctt cgaggtcgcg
     2461 acgcaaggct cgagcgacta ccgcgaggaa aagatcgacc tgcccgccgg ctggctgcgg
     2521 ggcctggcgc aggtgcaggc ggcgatgacg ctgccgatgc ggcgggtcag cctgactcgc
     2581 gaggcggttt attcgctgct ggcctggctc aagcggcaca aggctcagaa aagcccgcgg
     2641 gccctgcgat tcgaactgtg gcccgactgt ccgccgcggc tggtgctgga gccctgggag
     2701 actgccgttt actcgtacgg cacgacctac agcggcccgc ccggcgagcc gattcgcatc
     2761 tggggccggc agcgcttgct ggtgctggcc cgcttgctgc cgctggccga ccagttcgac
     2821 gtctacctgc tcggcacggg cctgcccagc ttctgggtcg cccgcatggg cgagatgcgg
     2881 ctgacggtcg gcctctccgg ctggacgacg aacgactgga cgcgcaacgc cgcgctcgac
     2941 gtggtgcggc cgccggtgaa gctgagcgaa gggctgactt ccggcgtggc ccaggcgatg
     3001 cgcacgcacc gcacgctcgc tccggccgcg accgcgctaa agtccgtcgc cagcgaagcc
     3061 gagtgcgccg cggcgctcaa tcacttggcg cacgccgggc aagtgatcta cgacctggcg
     3121 gccggcgtct atcgctggcg gcagatcatg ccgcgggcac tcggcgaggc cgagatcggc
     3181 ccggaaaacg cggagctcgc ggcctcgcgc gggctggtcg cgaaggtgaa gatcgactcg
     3241 cggcaggaag ctccgcgcgg cggcacggtc tatacggctg tgatcgaccg catgcagatc
     3301 gagatgctgc tcgacgccga cggcatcatc aagcgcggca agtgcaagtg ctcacaccat
     3361 ttcaagggcg gcctgcggat gggcccctgc cggcatttgc tggccgtgcg ggcggcggta
     3421 ctcggcggca cgcagcaggc gggcgacctg caggcctggt ttgcgcg
//
LOCUS       DASRJB010000716         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1059076, whole genome shotgun sequence.
ACCESSION   DASRJB010000716 DASRJB010000000
VERSION     DASRJB010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059076"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VFB80_18930"
     CDS             complement(<1..1854)
                     /locus_tag="VFB80_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN35915.1"
                     /translation="MTLTHRVLIALLVAVLAPAVALAQAKGKRPKSEAAADTSPQVAT
                     PIERIKTKPGFRVELLYSVPRSQGSWVSMCLDPQGRLIACDQGSGGLYRITPPPVGQA
                     ASLPADIKVEPLKLEISEAQGLLWAFDSLYVVVNSRARSGLWRAKDTDGDGELDKVEQ
                     LRALAGGGGEHGPHAVLLAPDGKSLYVVCGNQTRLTEFAKSRVPPIWGEDHLLPRMPD
                     GNGFMRGVLGPGGTIYQVDPDGKDWIVNTVGFRNEYDAAFNREGDLFTYDADMEWDFN
                     TPWYRPTRVCLAASGAEFGWRNGAGKWPAYYPDSLPAVVDIGPGSPTGVAFGYGAKFP
                     AKYQDALFINDWSYGKLYAVHLEPQGAGYTGTFEEFVTGTPLPLTDMVVRPQDGALYF
                     AIGGRNTQSGLYRVTYVGNESTAPAKGELKFADQRTIRRKLEGFHGKQDAAAVEAAWP
                     YLGSPDRYLRYAARVAIEHQDVNTWSAKALAEKDLPAALTALLALARVSGSDPFHRKD
                     RPLDPVSWQNGGIPTKDDVDPVTQVNDPRKREILEALAKLDWAKLTNQQRIDLLRVYG
                     VTFNRLCRPDAAQRQELLARFDPLYPAKGREPNAELSQLLVYLEAPSAPAKT"
BASE COUNT          338 a          704 c          701 g          367 t
ORIGIN      
        1 cgtcttcgcc ggtgcgctag gcgcctccag gtaaacgagc aactggctga gctcggcgtt
       61 cggctcgcgg cccttggccg gatagagcgg gtcaaaccgc gccaacaatt cctgccgctg
      121 ggccgcgtcg ggccggcaca agcgattgaa agtcacgcca tacacccgca acaggtcgat
      181 ccgctgctgg ttcgtcagct tcgcccagtc gagcttcgcc agcgcctcga gaatctcgcg
      241 cttccgcggg tcattgacct gcgtgactgg atcgacgtca tccttcgtag ggatgccgcc
      301 gttttgccac gagaccggat cgagcggccg gtccttgcgg tggaacgggt cgctgccgct
      361 gacgcgggcc agggccagca aagcggtgag cgcggccggc aggtctttct cggcgagcgc
      421 cttggcgctc caagtgttaa cgtcctgatg ctcgatcgcc acgcgggccg cataccgcag
      481 gtaacgatcg gggctgccca ggtacggcca ggccgcttcg accgcggcgg cgtcctgttt
      541 accgtgaaac ccctccagtt tgcggcggat tgtccgctgg tcggcgaatt tcagctcgcc
      601 cttggccgga gcggtcgatt cgttgccgac gtacgtcacg cggtacagcc ccgactgggt
      661 gttccgccca ccgatcgcga aatagagcgc tccgtcctgc ggccgcacga ccatgtcggt
      721 cagcggcagc ggcgtgccgg tcacaaactc ctcgaacgtg ccggtgtagc ccgcgccctg
      781 cggctccagg tgcaccgcgt agagcttgcc gtagctccaa tcgttgatga acagagcatc
      841 ctggtacttc gcgggaaact tcgcgccgta gccgaacgcc acgcccgtcg gcgagccggg
      901 gccgatatcg acgaccgccg gcagactgtc cgggtagtag gccggccact tgcccgcgcc
      961 gttccgccag ccgaattccg ccccgctggc cgccaggcag acccgcgtcg gccggtacca
     1021 gggcgtgttg aaatcccact ccatgtcggc gtcgtaggtg aacaggtccc cctcgcggtt
     1081 gaacgccgcg tcgtattcat tgcggaagcc gacggtgttc acgatccagt ccttgccgtc
     1141 cggatcgacc tggtagatcg ttccgcccgg ccccagcacg ccccgcatga agccgttgcc
     1201 gtcgggcatc cgcggcagca ggtgatcttc gccccagatc ggcggcacgc ggctcttggc
     1261 aaactcggtg aggcgcgtct ggttgccgca gacgacgtac aggctcttgc cgtccggcgc
     1321 gagcagcacg gcgtgcgggc catgttcgcc gccgccgccg gccagcgcgc ggagctgctc
     1381 gaccttatcg agctcgccgt cgccgtccgt gtctttcgca cgccagaggc ccgagcgggc
     1441 ccggctgttg accacgacat acagactgtc aaaggcccag agcagtccct gggcttcgct
     1501 gatttcgagc ttgaggggtt cgactttgat gtcggcgggc aagctggcag cttgccctac
     1561 gggagggggc gtgatccggt acagcccgcc gctgccctga tcgcaggcga tcagccggcc
     1621 ctgcggatcg aggcacatgc tgacccacga gccttggctg cgcggaacgc tgtagagcag
     1681 ttcgacgcga aatccgggct tcgttttgat ccgctcgatc ggcgtggcca cttgcgggct
     1741 ggtgtccgcg gccgcttcgg acttcggccg tttgcccttg gcctgggcca aggcgaccgc
     1801 gggcgcgagg actgcaacca acagcgcgat caggacacga tgtgtgagag tcatggcatt
     1861 agggcgaggg agtgccacga aaaatgaaag ggcccgtaca gcgtactcag tacggagtac
     1921 ggcgtacaag cttgagctgc gctggcacag ggcaagtcat cagccggccg ctgcgtggtc
     1981 cacggctggc acttcgctac tgtgcggcag cttgcggccg tgggacgata gcttgaacca
     2041 gacgacgagc agcgctgctt gtgcggccag caccagcgca gcgaccagga gcaagccgcg
     2101 atagctgatc
//
LOCUS       DASRJB010000717         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3033707, whole genome shotgun sequence.
ACCESSION   DASRJB010000717 DASRJB010000000
VERSION     DASRJB010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033707"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1115)
                     /locus_tag="VFB80_18935"
     CDS             complement(36..1115)
                     /locus_tag="VFB80_18935"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HZN35916.1"
                     /translation="MSARIGVCFASIILILAQSRPAPAQSLQPGVRGLATRAPAGMKI
                     DGDLSEFKGAFCTPVNYFVPAAQVKERPAQFFYMWDDTAFYAALRTLDTKTFNGSDDA
                     HLWGGDAVEWYFDTRREGDFRGIDWGKGAVHMYWTGFDKAELKPRWCLRPGYLDAIPG
                     KGVEVGAKKTEFGTDVEFKLPWENFPNFKAKLGEVIALDAELCYSDGAARVDRIFAYG
                     SPLCVQQPAAQGQVQLVEKLEPAHWKQCGAVLAPMRCDTPWTQRTKALVTGQVALPPD
                     HADQVGKIVFRVSDLEGKALGEYPAQRRTLHEAGRFYLAEAQWPSDVAVPGLHTVVAV
                     LHGSGGQELCRVAPRLVSAGWVQGY"
     gene            complement(1149..1829)
                     /locus_tag="VFB80_18940"
     CDS             complement(1149..1829)
                     /locus_tag="VFB80_18940"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN35917.1"
                     /translation="MSLSRSRRLQFEALESRLAATVVYNTTCLGPLLSIPAPGSSSRG
                     PAAMDDSFIIERGEVGAGSALLAVLSNDRTEPGDPLVVNYIECQQRYAGQASISYGRV
                     AIDYKPPAQFVGIDQFRYAVEDREGFGSSATITVEVRNGWRNQVRPNDVNGDAQVSPL
                     DALVVLNELNDRGSRQLGRPGPEATANRLDVDGNGFVNPLDALLVINELNAAAHMPAP
                     GQPSGQPG"
     gene            complement(1919..4657)
                     /gene="topA"
                     /locus_tag="VFB80_18945"
     CDS             complement(1919..4657)
                     /gene="topA"
                     /locus_tag="VFB80_18945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZN35918.1"
                     /translation="MPKRAASNPSGKALVIVESPAKARTISKFLGGNYLVEASIGHIR
                     DLPQGAKELPKEYKGETWAYLGVNVNDNFTPVYIVPPGKKSQVSKLKDLLKGSKELYL
                     ATDEDREGEAISWHLCEVLKPKVPVRRLVFHEITEEAIKDALASPRQIDDGLVRAQET
                     RRILDRLYGYEVSQLLWRKIGPKLSAGRVQSVAVKLIVEREKQRIAFRSATWWDLIGR
                     FATGEGKEFEAELISSEGRKIPTGKDFDPATGQIKDPQLLLLNEQQARELAERLMRGQ
                     FRVTNLEERPYTSRPPAPFTTSTLQQEANRKLGFGARRTMTIAQGLYEEGHITYMRTD
                     STNLAQVAIDAARDLVRTEYGGEYLPDSPRVYASKVKNAQEAHEAIRPAGHPFELPAV
                     LKGTLNTDQFRLFELIWKRTIASQMVDSRGRTITITVEADGSVFRVSGKTIDFPGFLR
                     AYVEGSDDPEAELADREQILPAVKIGDAVTCRELKAKDHTTQPPARFTEASLTAALEE
                     RGIGRPSTYASIIDTILERKYVFKKGSALVPTWTAFSVSKLLEQHLPTLVDYEFTALM
                     EDQLDEISRGEFEHVTYLKNFYFGNGRPGLKKQLDHKLQEVDPRDLSRFSLGKPAAGP
                     HREEIIVRVGKFGPFLEHGERKASIPQDLPPDELTQAKALEMLEQASVGEEPLGIHPD
                     THQPVYLKQGRFGPYVQMGAAGEDEKPKNASLLPGMTPADVTFEVAVKLLTLPRTIGD
                     HPVQHEPIVAFNGKYGPYIKCGSESRSLPKDLSPIDVTLEQAVHLLAQPKAQGRGRAA
                     PREPLKTFEASPVTNQPVKLLAGKYGNYVTDGTTNATLPKDLAPEELTFQQALDLLAE
                     RAARGPATPRGRRGRAAQAPKMPKTPKTPKAEKPAAEKKPKKAAKKSGRSKPPKSE"
     gene            4964..>6158
                     /locus_tag="VFB80_18950"
     CDS             4964..>6158
                     /locus_tag="VFB80_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35919.1"
                     /translation="MASKSMHFLRKYQKIVLVVMGVILMVTFTVGTSLSLLFDRQREG
                     RPTDPIALTWENGPVRESELQVMRYRHNAARQFLFTLIAETIQRGGRPVVNGQVITDL
                     QTLQRVGVGIQMDDSDPSLVTTMLMEAQARKQGIVVDREAVKAHLRRLTEPELREAEW
                     AEVMSNVLGEHSRLSADDVLDQLAYELRAQHAWLLANAGMGTVPPGKMWECFNRLNRR
                     AAIEAFPIDVEPYVKDVKGEPTPTELQNLFDEGRFRDPNPNSHEPGFRKPHKVAFEYV
                     KVDFAPFLETAMKEVTDEQIQKQYELNISQGKHKVQELPPATSGEAAKPAATPPADPK
                     PGETKPDEKKEPAEAKPAETKTGEAKPGDAKPADAKPADAKPEEATPANPAPECQEKK
                     AEDSAT"
BASE COUNT         1026 a         1933 c         2041 g         1158 t
ORIGIN      
        1 cgcgaccact ggtcgcggct ttgagaggtt gctacctagt agccctgcac ccagccggcg
       61 ctgaccaggc gcggggcgac gcggcagagc tcctggccgc cggagccgtg gaggacggcg
      121 acgacggtgt gcaggccggg cacggcgacg tcgctgggcc actgggcttc ggccagatag
      181 aagcggcccg cttcgtggag cgtccgccgc tgggccggat attccccgag cgccttccct
      241 tccagatcag aaacgcgaaa gacgattttg ccgacctgat cggcgtgatc ggggggcagg
      301 gcgacctggc cggtgacgag ggccttcgtc cgctgcgtcc agggcgtgtc gcagcgcatc
      361 ggcgcgagca ccgcgccgca ctgtttccag tgcgcgggct ccagtttctc gaccagttgc
      421 acctggccct gcgccgccgg ctgctggacg cagagcgggc tgccgtaggc aaagatgcgg
      481 tcgacgcggg ccgcgccgtc gctgtagcac agctccgcgt ccagcgcgat cacctcgccg
      541 agcttcgcct tgaagttcgg aaagttctcc cagggcagct tgaactcgac gtcggtcccg
      601 aattcggtct tcttcgcgcc gacctcgacc cccttgccgg gaatcgcatc gagatagccc
      661 ggccgcaggc accatcgcgg cttgagctcc gccttgtcga agccggtcca gtacatgtgg
      721 accgcgcctt tgccccagtc gatgccgcgg aagtcaccct cgcggcgagt atcgaaatac
      781 cactcgaccg cgtcgccgcc ccagaggtgc gcgtcgtccg agccgttgaa ggtcttggtg
      841 tcgagcgttc gcagcgcggc atagaacgcc gtgtcgtccc acatgtagaa gaactgcgcc
      901 ggccgctctt tgacttgggc ggccggcacg aagtagttga ccggcgtgca gaacgcgccc
      961 ttgaactcgc tcagatcgcc gtcgatcttc atgccggccg gggcgcgggt cgccaggccg
     1021 cggacgccgg gctgcagcga ttgagccggc gcgggccgcg actgtgcgag aatcaaaatg
     1081 atcgaagcga aacagacgcc aattcgtgcg gacatcggtg attgcctgca agagaaaggt
     1141 tgaacgggtc agccaggctg accgctcggt tggcccggtg ccggcatgtg cgcggcggcg
     1201 ttgagctcgt tgatcacgag caatgcgtcc agcgggttca cgaaaccatt accgtccacg
     1261 tccagccggt tggcggtcgc ctcgggaccg ggccggccca gttgccggga accgcggtcg
     1321 ttgagctcgt tcagcaccac cagggcgtcg agcggcgaga cctgcgcgtc gccgttcacg
     1381 tcgttcggcc gaacctggtt ccgccagccg ttgcggactt cgacggtgat cgtggcgctg
     1441 ctgccgaagc cctctcggtc ttccaccgca tagcggaact ggtcgatgcc aacgaactga
     1501 gccggcggct tgtagtcgat ggccactctt ccgtacgaga tgctggcttg tccggcgtag
     1561 cgctgttggc actcgatata gttcaccacc aggggatcgc cgggctcggt gcggtcgttg
     1621 gagagcactg ccaacagcgc gctccctgct cccacttccc cccgttcgat gatgaacgag
     1681 tcatccatcg cggcggggcc gcgggaactg gacccgggcg ccgggatgga aaggagagga
     1741 ccgaggcagg tggtgttgta gaccacggtc gccgctaagc gcgactccag cgcctcaaat
     1801 tgcagtcgtc gcgaccggga aaggctcatg ccatccgacc tcgggtcaat gcagtcgccc
     1861 taaggtattc gccaccgcgt acaaagtcaa agatgcagat cttcggccgg accagaggtc
     1921 attccgattt aggcggcttt gagcggccag atttcttcgc ggccttcttg ggcttcttct
     1981 cggcggcggg cttctccgcc ttcggcgtct tgggcgtctt tggcatcttc ggagcttggg
     2041 ctgcccggcc gcggcggccg cgaggtgtcg cggggccgcg ggcggcccgt tcggcgagca
     2101 ggtcgagggc ctgctgaaag gtcagctctt cgggtgcgag gtccttgggc agcgtggcgt
     2161 ttgtcgtgcc gtcggtcacg taattgccat acttgcccgc cagcagcttc accggctggt
     2221 tggtgaccgg ggaagcctcg aacgtcttga gcggctcgcg cggcgcggcg cggccccggc
     2281 cttgagcttt cggctgggcg agcaggtgga cggcctgctc cagcgtgacg tcgatcggcg
     2341 acaggtcctt cggcagcgac cgcgattcgc tgccgcactt gatgtacggc ccatacttgc
     2401 cgttgaaggc gacgatcggc tcgtgctgca cggggtgatc gccaatggtc cgcggcagcg
     2461 tcagcagctt cacggccact tcaaacgtga catcggcggg cgtcatgccc ggcaggagcg
     2521 acgcgttctt cggcttctcg tcttcgccgg ccgcacccat ctgcacgtac gggccaaaac
     2581 gcccctgctt cagatagacc ggctggtgcg tgtccgggtg aattcccagc ggctcctcgc
     2641 cgacgctggc ctgctcgagc atctccaggg ccttggcctg ggtcagttca tccggcggca
     2701 agtcttgcgg aatgctcgcc ttccgctcgc cgtgctccag gaacgggccg aacttgccca
     2761 cgcggacgat aatctcttcg cggtgcgggc ccgccgccgg cttgcccagc gaaaaccggc
     2821 tcaaatcgcg cggatcgact tcctggagct tgtggtcgag ctgcttcttc agacccggcc
     2881 ggccgttgcc gaaatagaaa ttcttcaggt aagtgacgtg ctcgaattcg ccgcggctga
     2941 tctcgtcgag ctgatcttcc atcagcgcgg tgaactcgta atcgacgagc gtcggcaagt
     3001 gctgctcgag cagcttcgac acggagaacg ccgtccaggt cggcaccagg gcgctgccct
     3061 tcttgaaaac gtattttcgc tccaggatgg tgtcgatgat cgaggcgtag gtgctcggcc
     3121 ggccgatgcc gcgctcttcg agggcggcgg tcagcgaggc ttcggtgaac cgcgccggcg
     3181 gctgcgtggt atggtccttg gccttgagct cgcggcaggt cacggcgtcg ccgattttca
     3241 ccgcgggcag aatctgctcg cgatcggcca gctcggcctc gggatcgtcg gagccttcga
     3301 cgtaggcccg caggaagccg gggaagtcga tcgtcttgcc gctgacgcga aacaccgagc
     3361 cgtcggcctc gaccgtgatc gtgatcgtcc ggccgcggga atcgaccatc tggctggcga
     3421 tggtccgctt ccagatcagc tcgaacaggc gaaactggtc ggtgttgagc gtgcccttga
     3481 gcaccgccgg cagttcgaac gggtgccccg ccgggcggat cgcttcgtgg gcctcctggg
     3541 cgttcttgac cttcgaggca tagactcgcg gactgtccgg caaatattcg ccgccgtatt
     3601 cggtgcggac caggtcgcgg gcggcgtcga tcgccacctg ggccaggttc gtcgagtccg
     3661 ttcgcatgta ggtgatgtgg ccttcttcat acaggccctg ggcgatggtc atcgtccgcc
     3721 gcgcgccgaa gccgagcttg cggttggcct cttgctgcag cgtgctggtg gtgaagggcg
     3781 cgggcggccg gctggtatag ggccgctcct ccaggttcgt gactctaaac tggccccgca
     3841 tcagcctttc agccagctcg cgcgcctgct gctcgttcag gagcagcagt tgcgggtcct
     3901 tgatctggcc ggtggccgga tcgaagtcct tgccagtcgg aatcttgcgg ccctcgctgc
     3961 tgatcagctc ggcttcgaac tccttgcctt cgccggtggc aaagcggccg atcaaatccc
     4021 accaggtcgc ggagcggaat gcgatccgct gcttctcgcg ctcgacaatc agctttacgg
     4081 cgacgctctg cacgcggccg gcggatagct tggggccaat tttccgccag agcagttgcg
     4141 agacttcgta gccgtaaagg cggtcgagaa tgcggcgcgt ttcctgggcc cggacgaggc
     4201 cgtcatcgat ctggcgaggg ctggccaggg cgtccttgat cgcttcttct gtgatttcgt
     4261 ggaaaacgag ccgccggacc ggcaccttgg gcttcagcac ctcgcacaaa tgccagctga
     4321 tcgcttcccc ttcgcgatct tcgtccgtcg ccaggtagag ctctttactg cccttcagaa
     4381 ggtctttcag cttcgagact tgcgactttt ttccgggggg cacgatatac acgggggtga
     4441 agttgtcgtt gacattcact cccaggtagg cccacgtttc tcctttatat tccttgggca
     4501 gttccttcgc gccctgcggc aggtcgcgaa tgtggccgat gctcgcctcg acgagataat
     4561 tgccacccag gaacttcgag atcgtccgcg ccttggcggg tgactcgacg atcacgagcg
     4621 ctttgccact cgggttggag gcggcgcgtt tgggcatgat ctctctttca ccgtaagcga
     4681 atgcgactat ccttggtcgg cacgatcctc acctgtctga gtcggcatcc tggttattgc
     4741 cggcggcatt gttgcctgag aactcgcttt ggcaatggat catagcccag cgccgttggc
     4801 gtacgtgctg attgggattg aatttacgtc gcgccggcat acaatctgcc agctttggca
     4861 gaccttgacg gttgggccaa ctggatcgaa aaatcattta tcgccgagcg gcagccttgt
     4921 caacaggctc tcgcgggcgg cgtctttgcg aagggcgagc ggtatggcca gcaagtcgat
     4981 gcattttctg aggaaatatc aaaagatcgt gctggtggtc atgggtgtca tcttgatggt
     5041 gacatttacg gttggcacct cgctttccct gctgttcgac cggcaacgcg agggccggcc
     5101 gaccgatccg attgccctga cctgggagaa tggccctgtc cgcgagagcg agctccaggt
     5161 catgcggtac cggcacaatg ccgcccggca gttcctgttt acgctcattg ccgagaccat
     5221 ccagcgcggc gggcggccgg tggtcaacgg gcaggtgatt accgatctcc agacgctcca
     5281 gcgggtcggc gtcggaattc agatggacga cagcgatcca tcgctcgtca ccacgatgtt
     5341 gatggaggca caggcgcgga agcagggcat tgtcgtcgat cgcgaagcgg tgaaggcgca
     5401 cctccggcgg ctcaccgagc cggagctgcg cgaggccgaa tgggccgagg taatgagcaa
     5461 cgtgctcggc gagcacagcc gcctttcggc cgacgacgtg ctcgatcaat tggcgtacga
     5521 gctccgggcc cagcatgcct ggctgctggc caatgcgggc atgggaacgg tgccgccggg
     5581 gaaaatgtgg gaatgcttca accggctcaa tcgccgcgcc gcgatcgagg cgtttccgat
     5641 cgacgtggag ccgtacgtca aggatgtgaa aggggagcct acgccgacgg agcttcaaaa
     5701 cctgttcgac gaaggccgct ttcgcgatcc gaacccgaac tcgcacgagc ccggcttccg
     5761 caagccgcac aaggtcgcgt tcgagtatgt gaaggtcgat ttcgcgccgt tcctcgagac
     5821 ggcgatgaag gaggtgaccg acgaacaaat ccagaagcag tacgagctga acatcagcca
     5881 gggcaagcac aaggtgcagg agctgccgcc ggcgacctcc ggcgaagcgg ccaagcccgc
     5941 agcgactcca ccggcggacc ccaaaccggg cgagaccaaa cccgacgaga aaaaagaacc
     6001 ggccgaggcg aagcctgcgg agaccaagac tggcgaggcc aagccgggcg acgcgaagcc
     6061 tgccgatgca aagccggcgg atgcaaagcc ggaggaggcg acgccggcca atccagcgcc
     6121 ggagtgccag gagaaaaaag ctgaggattc cgcgacgc
//
LOCUS       DASRJB010000718         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_774404, whole genome shotgun sequence.
ACCESSION   DASRJB010000718 DASRJB010000000
VERSION     DASRJB010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774404"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFB80_18955"
     CDS             complement(<1..742)
                     /locus_tag="VFB80_18955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZN35920.1"
                     /translation="MQDKLTRQGITFDDVLLEPRFSDAVPTECSVTTKLTRGIQLNIP
                     LLSSPMDTVTEHEMAIALAREGGLGIIHKNMSIEQQSEEVNKVKRSANGIILDPVTLR
                     PDEPVSRAQELMKLKNVSGFPITDSSGKLVGILTRRDLRFLENGQQPIREVMTKDNLV
                     TARGTVTLEEAERILTAKKVEKLLLVDDTYCLTGMITIRDIDMMKRFPHACKDRLGRL
                     RVGGAVGVFDLERAHSLIQTGVDILVVDS"
     gene            complement(818..1798)
                     /locus_tag="VFB80_18960"
     CDS             complement(818..1798)
                     /locus_tag="VFB80_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35921.1"
                     /translation="MSTVSPSEPAKTGRRRFRLPLRFSLRAFLAAVTIFCIALAWQLH
                     RAKLQREAVAAIRAAGGWVHYDYQHPSYPTYQIDAAARPWEPAWLLALVGTDFFHDVT
                     DVNMVYNEEGPQRLDNTRPPVDISPQLARFPRLRSLAISGDFLDDEGMRAVGRLKRLE
                     TFYQWNGGAIGDDGAAYLHDMPRLRYIHLGNSRVGDRGMQTLAKLPNLEGISMQVNRV
                     TDAGLAALAGHPKLKTLWIGSLNQMSPISDAGVVHLAKVPQLEELDLQFTRVTPEGLK
                     PLQKLPNLKSLLLSGSTADNLPAVAPLLPNCRIDAKTPPPITAPPMQPAD"
     gene            complement(1910..2146)
                     /locus_tag="VFB80_18965"
     CDS             complement(1910..2146)
                     /locus_tag="VFB80_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35922.1"
                     /translation="MKSTTTNRLRMLLAGVGLAALTLGTGCQVVENGQTLPSPWYYYD
                     DIQYFAPGPEFKLTNEAAAQKAYKADLEAQAIRP"
     gene            complement(2333..3700)
                     /locus_tag="VFB80_18970"
     CDS             complement(2333..3700)
                     /locus_tag="VFB80_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35923.1"
                     /translation="MLLRSANGFGAVALAALLSDRAYGGPLSPRAGHYAARARSVIFL
                     YMDGGPSQVDTFDPKPRLAREHGQPIKMAAPPTQFIPIDRAPTVLASPWKFQPRGQCG
                     TPVSDLLPHIASRADDLCVVRSMVANFTEHANANLFLHTGSNQQGRPSVGSWVTYGLG
                     TESQELPGYIVLKGGQIPAGGPDNFHSGYLPAVHQGSIFRDPVAPVANLQRTEPTDAL
                     QRGKLDLLAKLDQSVLGRGDPRIEAAIANYELAFRMQTAVPELLAVSSETAATERLYG
                     LDDEATRPFGTQCLLARRLIERGVRFVEVMPPAVAGANRWDQHDRIADGHAKMARATD
                     LPIAGLLADLKARGLLDQTLVVWAGEFGRTPVAQGASRGRDHSPYGFSIWLAGGGVRG
                     GQVYGATDEYGFHAIENKVEIHDLHATMLHLLGIDHTRLTYRFGGRDMRLTDVHGNVL
                     HEILA"
     gene            complement(3750..>6524)
                     /locus_tag="VFB80_18975"
     CDS             complement(3750..>6524)
                     /locus_tag="VFB80_18975"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN35924.1"
                     /translation="IRHKLDAAGLSPAPPAERRTLIRRLTFDLTGLPPTPADVEAFLA
                     DGSPDANERLVDRLLASPQYGERWARHWLDVVRYSETLGFEFDYDLYHAWRYRDYVIR
                     AFNADLPYDQFVIEHLAGDLVAEPRRAADGSNGSILATGFFWMLEGKQTPVDIRQEQA
                     DRIDNQIDTLGKAFLGQTIACARCHDHKFDAIRTRDYYALAGYLRSSRYQQAFIDPPE
                     RRATQIAELAALREQIAARAKAELTGQWRQILAPADRYRQAAQRIAAGMDGVTERDRA
                     DALQAAAAESGLDADRLQRWVTALKEVEPTSREPAPASSAGKQLEDFSGSTFDGWFVT
                     GDAFSSGPVSPGEIVVGEKAERPIARLTTGGADSGALAPRLQGELRSATFTIDKPMLH
                     VRLAGKNARVNLVIDGNTLIMDPMYGKLTTIPDNTLPEWRTMPVGRWLGRRAYLEISD
                     STIPMHRLNPPPSSAHVPVGKGDGYVAIDQICLSDDRRPPQEAHESLPATADQQRMLQ
                     AVDRWQRGAAGEADLLLLNWLLDAGVLDTKAASAELTELVKHYRDLAASLPLPERAPA
                     LADGTGEDEFVFLRGNYRTPGERATRGPPEVLCAAGNATEGGNATEARNATEGIPYSA
                     VGGSGRLELAKRVASPENPLLARVLVNRLWQHHFGAGLVLTPDDFGRMGQPPSHPELL
                     DHLADEFTRAGWSIKAMHRRLVNSTTYRMSSALPQSEISNLKSEIGAVDPDNRLLSHM
                     PVRRLEAEAVRDSLLAISGRLDRTQHGPSVLPFLTQYMEGRGRPQSGPLDGDGRRSIY
                     LNARRNFLTPLLLTFDYPVTFTCIGRRGGSAIPAQALALMNDPFVVQQAGQWAQRAIA
                     EGGATSESRIDWLYGTGLSRRPTENERAAALEFLQQQAARHGCPADDARVWTDLCHVL
                     INVKEFIFID"
BASE COUNT         1005 a         2094 c         2283 g         1142 t
ORIGIN      
        1 ccgaatcgac gaccaggata tcgacacccg tctggatcag gctgtgggcc cgctccaggt
       61 cgaacactcc caccgcccca ccgacccgca gcctccccag cctgtctttg caagcatggg
      121 gaaaccgctt catcatgtcg atgtcgcgga tggtgatcat gccggtcagg caatatgtat
      181 cgtccaccaa caacaatttc tcgacctttt tcgccgttaa aattcgctca gcttcctcaa
      241 gcgtcacagt cccccgcgcc gtgacgaggt tgtccttggt catcacctcg cggatcggct
      301 gctggccgtt ctcgagaaac cgcaggtcgc ggcgggtgag gatgcctact agcttgccgc
      361 tgctgtcagt gatgggaaag ccgctgacgt tcttcagctt catcagctcc tgggcccggc
      421 tgacgggctc gtcggggcgc agggtgaccg gatcgaggat gatgccgttg gccgaccgct
      481 tgaccttgtt gacctcctcg gactgctgct cgatcgacat gttcttatgg atgatgccca
      541 gcccgccttc ccgggccagc gcgatcgcca tctcgtgctc ggtcaccgta tccatgggcg
      601 agctgagcag cgggatgttg agctgaatcc cccgcgtgag cttcgtcgtc acgctgcact
      661 ccgtcggcac cgcgtcgctg aaccgcggct cgaggagcac atcgtcgaac gtgattccct
      721 ggcgagtcag cttgtcttgc atgcgaggtc ctccgtgggg aatcccgtat tatcgcccgc
      781 cgcggcgaag cgggcgaggg gacgagcgct ccgagagcta gtctgccggc tgcatcggcg
      841 gggcggtgat cggcggcggc gttttggcgt cgatgcgaca gttgggcaat agcggagcga
      901 cggcgggcag gttgtcggcc gtgctgccgc tgagcagcag cgacttcaaa ttcggcagct
      961 tttgcagcgg tttcagcccc tcaggcgtca cgcgggtgaa ttgcaggtcg agctcctcga
     1021 gctgcggcac cttcgccagg tggacgacgc ccgcgtcgct gatcgggctc atctggttga
     1081 ggctgccgat ccagagcgtc ttcagcttcg ggtggccggc gagtgccgcc aggccggcgt
     1141 cggtgacgcg gttcacctgc atgctgattc cttcgaggtt aggcaatttg gccagcgtct
     1201 gcatcccacg gtcgccgacg cgcgagttgc cgaggtggat gtaccgcagc cgcggcatgt
     1261 cgtggaggta ggccgcgccg tcgtcgccga tcgccccgcc gttccattga tagaacgtct
     1321 ccagccgctt gagccggccg acggcccgca tcccttcgtc gtcgagaaag tccccgctga
     1381 tcgccagcga ccgcagccgc gggaagcggg ccagctgggg cgagatgtcc acgggcgggc
     1441 gagtgttgtc gagccgctgc ggtccctcct cgttgtagac catgttgacg tcggtcacat
     1501 cgtggaagaa gtcggtcccc acgagcgcca acagccaggc gggctcccag ggtcgggccg
     1561 cggcatcgat ttgatacgtg gggtagctgg ggtgctgata gtcgtagtgc acccagccgc
     1621 cggcggcgcg gatggccgcg actgcctcgc gctgcagctt cgcccggtgc agctgccagg
     1681 ccagggcgat gcagaagatc gtcaccgcgg ccaggaacgc gcggaggctg aaccgcagcg
     1741 gcaggcggaa gcgccgccgg cccgttttcg ccggttcgct gggcgaaact gtgctcatcg
     1801 ccccattttg cgaagcggcg ctggctcaag caacccgaag cgtcagcgag atactctgcc
     1861 ggtcagcggg gtttcagaaa gtcccttgct tacgcgtcgg gttagtgtgc tatggccgga
     1921 tggcctgggc ttcgagatcg gccttgtagg ccttctgggc cgcggcctca ttggtgagct
     1981 tgaactcggg gccgggcgcg aagtactgga tgtcgtcgta gtagtaccac gggctgggca
     2041 gcgtctggcc gttctccacg acctggcagc ccgtgcccag cgtcagcgcg gccaggccga
     2101 ctccagcgag cagcatccgc aggcgattgg tcgtggtcga tttcatggat ggcatcctga
     2161 ttggcccgca gaaccggccc agccgtcaaa gtcggtgcaa acgtcacggt ctatatcggc
     2221 cgcgggccag ccgaactttg gccggggcgg agagatttgc ggaatcgggc gcaaggacgc
     2281 tagcccgccg acgctggcag cgacggccgc gtagacgatg ctgccagcat cgtcacgcca
     2341 gaatctcgtg cagcacgttg ccgtgcacgt cggtcagccg catgtcgcgc ccgccgaagc
     2401 ggtacgtcag ccgcgtgtga tcgatgccca acaggtgcag catcgtggcg tgcaggtcgt
     2461 ggatttcgac cttgttctcg atggcgtgga aaccgtactc gtcggtcgcg ccgtagacct
     2521 ggccgccgcg gacgccgccg ccggccagcc agatcgagaa gccgtagggg ctgtggtcgc
     2581 ggccgcggct ggcgccctgc gcgacgggcg tgcggccgaa ctcgccggcc cacacgacga
     2641 gcgtctggtc caggaggccg cgggccttca ggtcggccag caggcccgcg atcggcaggt
     2701 cggtggcccg ggccattttg gcgtggccgt cggcgatccg gtcgtgctgg tcccagcggt
     2761 tggcccccgc cacggcgggc ggcatgactt cgacgaaccg cacgccccgc tcgatcagcc
     2821 ggcgggccag caggcactgc gtgccgaacg ggcgcgtcgc ttcgtcgtcc aggccgtaca
     2881 gccgctcggt ggccgcggtc tcgctgctta ccgccagcag ctccggcacg gcggtctgca
     2941 tccggaaggc cagttcgtag ttggcgatgg cggcttcgat ccgcggatcg ccgcggccca
     3001 acaccgactg atccagcttc gcgagcagat cgagcttgcc gcgctgcaat gcgtcggttg
     3061 gctcggtccg ctgcaggttg gcgacgggag cgaccggatc gcgaaagatc gaaccctggt
     3121 gcaccgctgg caggtagccg ctgtggaaat tgtcggggcc gcccgctgga atctgaccgc
     3181 ccttgagcac gatgtagccg ggcagctcct ggctctcggt gcccaggccg taagtgaccc
     3241 acgagccaac gctgggccgg ccctgctggt tcgagccggt gtgcaaaaag aggttggcgt
     3301 tggcgtgctc ggtgaagttg gcgaccatcg agcggacgac gcacaagtcg tcggcccggg
     3361 aggcgatgtg cggcaagagg tcgctgaccg gcgtgccgca ctggccgcgg ggctggaatt
     3421 tccagggcga agccaacacc gtcggcgcgc gatcgatcgg gatgaactgc gtcggcggcg
     3481 cggccatctt gattggctgg ccgtgctccc gcgcgaggcg cggcttgggg tcaaaagtat
     3541 cgacctgcga cgggccgccg tccatgtaga ggaagatgac gcttctggcc cgcgcggcgt
     3601 aatgtccggc ccgcggtgaa agcgggccgc catacgctcg gtccgagagc agcgcggcga
     3661 gcgcgaccgc gccgaagccg ttggcgctgc gcaaaagcat ctcgcgccgc gagagcggtg
     3721 ggcagtggcg gcagtcgttt tgaaatgacc tagtcaatga agataaactc cttgacgttg
     3781 atcagcacgt ggcacaggtc ggtccagacg cgggcgtcgt ccgcggggca gccgtggcgg
     3841 gcggcttgct gctggaggaa ctccagggcg gccgcacgtt cattttccgt cggccggcgg
     3901 gacaatccag tcccgtagag ccagtcgatc cgcgactcgc tggtcgcgcc cccttcggca
     3961 atcgcccgct gggcccactg gccggcctgc tggacgacga aggggtcgtt catcagcgcc
     4021 agcgcctggg ccggaatggc cgatccgccg cggcggccga tgcaggtgaa ggtcacggga
     4081 tagtcgaacg tgagcaggag cggcgtgagg aaattccgcc gggcgttgag atagatcgag
     4141 cggcggccgt cgccgtcgag cggccccgac tgcggccggc cgcggccttc catgtactgc
     4201 gtcaagaacg gcagcacgct tggcccgtgc tgcgtgcgat ccaggcggcc ggagattgcc
     4261 agcagcgaat cgcggaccgc ttcggcctcc aggcggcgaa cgggcatgtg ggaaaggagg
     4321 cgattgtcgg gatcgacggc gccaatttca gatttcagat ttgagatttc agattggggc
     4381 agcgcagacg acatgcggta cgtggttgag ttaaccaacc gccggtgcat cgccttgatc
     4441 gaccagccgg cgcgggtgaa ttcatcggcg aggtggtcga gcagttcggg gtggctgggc
     4501 ggctggccca tgcgaccgaa gtcgtcgggc gtgaggacca gccccgcgcc gaaatggtgc
     4561 tgccagagtc ggttgaccag cacgcgagcc aggagcggat tctctggact ggctactcgc
     4621 ttggccaact ccagccgccc gctgcccccc acagcgctgt agggtatgcc ctctgtggca
     4681 ttccgggcct ctgtggcatt cccgccctcg gtggcgttcc ccgcggcaca gagcacctcc
     4741 ggaggcccgc gcgtcgcccg ctcgccgggc gtgcgataat tcccgcggag gaacacaaac
     4801 tcatcctcgc ccgtgccgtc cgcgagggcc ggcgcacgct ccggcagcgg caagctcgcc
     4861 gccaggtcgc gatagtgctt cactagctcg gtcaattcgg cgctcgctgc ctttgtatcc
     4921 agcacgcccg catcgagcag ccaattgagc agcagcaaat cggcctcgcc cgccgcgccg
     4981 cgctgccagc gatcgaccgc ctgcagcatc cgttgctgat cggcggttgc cggcagggat
     5041 tcgtgagctt cctgcggtgg acgacggtcg tcggaaaggc agatctgatc gatggcgacg
     5101 tagccgtcgc cttttccgac ggggacgtgg gcgctggagg gcggcggatt gaggcggtgc
     5161 atcggaatcg tgctgtcgct gatttccagg taggcccgcc gcccaagcca gcggccgacc
     5221 ggcatcgtcc gccactctgg cagtgtgtta tctggaatgg tcgtgagctt gccgtacatc
     5281 gggtccatga tcagcgtgtt gccgtcgatg accaggttca cgcgggcgtt cttgccggcc
     5341 aggcgcacgt gcagcatggg cttgtcgatc gtgaaggtgg cggagcgcag ctcgccttgc
     5401 agccgcgggg cgagcgcgcc gctgtcggcc ccgcccgtcg tcaggcgggc gatcggccgc
     5461 tcggcctttt cgccgacgac gatttcgcca ggcgaaactg gtccggagct aaacgcgtcg
     5521 ccggtcacga accagccgtc gaaggtggag ccgctgaaat cctccagctg cttacctgcg
     5581 gaactggccg gcgcgggctc gcgcgaagtc ggctcaactt ctttgagagc cgtgacccag
     5641 cgctggagcc ggtcggcgtc caggccagat tctgccgctg ccgcttgcaa agcatcggcg
     5701 cggtcgcgct cggttacgcc gtccatgcca gcagcgattc tctgtgccgc ctgccgatac
     5761 cggtcggccg gcgccaggat ttgccgccac tgcccggtca actcggcctt agcccgtgcg
     5821 gcgatttgct cgcgcagggc ggccaactct gcaatttgcg tcgcccgccg ctcgggcgga
     5881 tcgatgaacg cctgttgata ccggctgctg cggaggtagc cggccagggc gtaatagtcg
     5941 cgggtgcgga tggcgtcgaa cttgtggtcg tggcagcggg cacaggcgat cgtctggccg
     6001 agaaaggcct tgcccagcgt gtcgatctgg ttgtcgatcc ggtcggcctg ctcctggcgg
     6061 atatcgaccg gcgtctgctt gccttcgagc atccagaaaa agccggtcgc caggatcgac
     6121 ccgttcgagc cgtccgcggc ccgtcgcggt tctgctacca ggtcgccggc caggtgctcg
     6181 atcacgaact ggtcgtaggg cagatcggcg ttgaaggccc ggatgacgta gtcgcgatac
     6241 cgccaggcgt ggtagaggtc gtagtcgaat tcgaaaccga gcgtctcgct gtagcggaca
     6301 acgtcgagcc agtgccgggc ccagcgctcg ccatattgcg ggctggcgag caggcgatcg
     6361 accagccgtt cgttagcatc gggcgagccg tccgccaaaa acgcctcgac atcggctggc
     6421 gtcggcggca ggccggtgag gtcgaacgtc agccggcgga tcagcgtccg gcgctcggcg
     6481 ggcggcgcgg gcgacaggcc ggcagcgtcg agcttgtggc gaat
//
LOCUS       DASRJB010000719         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1334528, whole genome shotgun sequence.
ACCESSION   DASRJB010000719 DASRJB010000000
VERSION     DASRJB010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334528"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFB80_18980"
     CDS             complement(<1..1134)
                     /locus_tag="VFB80_18980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35925.1"
                     /translation="MPAAIPARPDAEQRLREACAQLRQRVREGDERALEEILAQFPEL
                     AGDPDQALELVYAEVALREQLGRQLPPAAWLDRFPQWRERLHKLLEVHQALRADGAGR
                     TVDTSLSAAGQTPHTAASGTELAGLPKPRGYEVLEELGRGGMGVVFKARQVGLNRLVA
                     LKMILAGEFASDAERSRFRAEAEATARLDHPHIVRVHEVGEQEGQLFLAMEYVAGGTL
                     AEVFASGPQSADEAARLVELLARAVEHAHQQGIIHRDLKPGNILLQGKASPKIVDFGL
                     AKRFSNATTAGHGAATATGALLGTPSYMAPEQAAGGAVPVGPAVDVYALGAILYEALT
                     GRPPFRGQTALDTLDQERSHEPVPVRRLQPRVPRDLETICLKCL"
     gene            complement(1197..>2581)
                     /locus_tag="VFB80_18985"
     CDS             complement(1197..>2581)
                     /locus_tag="VFB80_18985"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HZN35926.1"
                     /translation="LLVHREDVGGRWITTRSIDFSGDGVIDPATESGWYVFDDLRPGV
                     YFVRTLLVIGFPIPGRIQTSPPFDPPKPELIGEVTSGPAEPPAPGLLPDLTLELPHGL
                     GDWFLTGDVLHFGQATPNIGAGPMDIIAGPDQGNGTRLVYQRIYNEPELLTYTDYEAG
                     TVSFHPEHNHTHLDDYARFSVLEALPDSTGDGIPEVGAEVVEGLKSSFCLADTMPYDL
                     SLPNAAQEPSFFGCVDQQRLSVGWMDVYDPLTPGQQIDVGSLAPGQYWLQGEVDPENH
                     IRELNENNNVGRVLITVGLGVPSAPGGAIGVQLASGQTAADNDFGNFQEISISGQVFH
                     DKNENGQFDPSDKKLPGRIVFLDLNGDGVLNNPVEGDNVASPFADEPWAIADKHGRYV
                     LSGVGPGEYAVRQILPEGWSQSTFDPDPVFALSGQDVPNIDFGSILGLLDDLDWLNLP
                     PGRRRSPERG"
BASE COUNT          414 a          850 c          870 g          447 t
ORIGIN      
        1 caggcacttg agacaaatcg tctccaggtc gcgcggcacg cgcggctgga ggcggcgaac
       61 cgggacgggc tcgtgcgacc gctcctggtc gagcgtgtcc agcgccgtct ggccgcggaa
      121 gggcggccgt ccggtaaggg cctcgtacaa gatcgcgcct agggcataga catcgaccgc
      181 agggccaacc ggcactgcgc cgccggctgc ctgttcgggc gccatgtagg acggagtgcc
      241 gagcagcgcg ccggtggcgg tggcggcacc gtggcccgcc gtcgtggcat tggagaatcg
      301 cttggccagg ccgaaatcca cgattttggg actggccttg ccctggagca ggatgttccc
      361 gggcttaaga tcgcgatgga tgatcccctg ctggtgcgcg tgctcgaccg cgcgtgccaa
      421 cagctcaacc aggcgcgccg cctcgtcagc ggattgcggc ccagaggcaa acacttccgc
      481 cagcgtgccg ccggcgacgt attccatggc cagaaacagc tggccctcct gctcgccgac
      541 ttcgtgcacc cgcacgatgt gcggatgatc aaggcgagcg gtcgcctcgg cctccgctcg
      601 aaaacggctc cgctcggcgt cgctggcaaa ttcgcccgcg aggatcatct tcaaggccac
      661 caggcggttc agccccactt gccgggcctt aaagacgact cccattccgc cgcggccgag
      721 ttcctccagc acttcgtatc cgcgaggttt gggtaggcca gcaagctccg ttccagatgc
      781 tgccgtgtga ggcgtctgac cggcagcgga cagcgacgta tccacagtcc gccccgcgcc
      841 gtccgcacgc agggcctggt ggacttccag cagcttgtgt aaccgctccc gccactgcgg
      901 gaaccggtcc agccacgccg caggcggcag ctggcggccc agctgctcgc gcagtgcgac
      961 ttcggcgtaa accagctcca gggcctggtc ggggtcgccg gcgagctccg gaaattgagc
     1021 gaggatttct tccagcgccc gttcgtcccc ctcgcgcacc cgttggcgga gctgggcgca
     1081 ggcttcgcgc agtcgctgct cggcatccgg acgggcgggg atcgccgcag gcatttcacg
     1141 ctcccgggaa aacgcacggc gccatgaacc tacttcgctc gcggctggtc atcaagttag
     1201 ccgcgctcgg ggctgcgccg ccgccccggc ggcagattca gccaatcgag atcgtcgagc
     1261 aggccgagaa tgctgccgaa atcgatgttg ggcacgtcct ggccgctcag cgcgaacacg
     1321 ggatcgggat cgaacgtcga ttgcgaccag ccttcgggga ggatttgccg cacggcatat
     1381 tcgcccggcc ccacgccgct caggacatag cggccgtgct tgtcggcgat cgcccagggt
     1441 tcgtcggcaa acggggaggc gacgttgtcg ccttcgacgg ggttgttcag cacgccgtcg
     1501 ccgttgaggt ccaggaacac gatccggccc ggcagtttct tgtcgcttgg atcgaactgg
     1561 ccgttttcgt tcttgtcgtg gaagacctgg ccgctgatgc tgatctcctg gaagttgcca
     1621 aagtcgttgt cggctgcggt ctggccgctc gccagctgca ctccgattgc cccgcccggg
     1681 gcggacggaa cgccgaggcc gaccgtgatc aggacgcggc ccacattgtt gttctcgttg
     1741 agctcgcgga tgtggttttc cgggtccact tccccctgca gccagtattg gccgggcgca
     1801 aggctgccga cgtcgatctg ctggcccggc gttagcgggt catagacgtc catccagccg
     1861 accgacagcc gctgttgatc gacgcagccg aagaagctcg gctcctgagc ggcgttgggc
     1921 agcgacaggt cgtagggcat cgtatcagcc aggcagaagc tgctcttgag tccttcgacg
     1981 acctcggcgc ccacttccgg aattccgtcg ccggtcgaat cgggcagcgc ttccagcacg
     2041 ctgaagcggg cgtaatcgtc caggtgcgtg tggttgtgct cggggtggaa cgaaaccgtg
     2101 ccggcttcgt agtccgtgta cgtgagcagc tccggctcgt tgtagattcg ctgataaacg
     2161 agccgcgtgc cgtttccctg gtcggggcca gcgatgatgt ccattggtcc cgcaccgatg
     2221 ttcggcgtgg cctgaccgaa atggagcacg tcgccggtca ggaaccagtc gcccaggccg
     2281 tgcggcagct cgagcgtcag gtcgggcagc aacccgggag cgggaggttc ggcggggccg
     2341 ctggtgactt cgccgatcaa ctccggcttg ggcggatcga acggcggcga ggtctgaatg
     2401 cggcctggaa tggggaagcc gatcaccagc agcgttcgca cgaagtacac gccggggcgc
     2461 aggtcgtcga acacatacca gcccgactcc gtggccggat cgatgacgcc gtcgccgctg
     2521 aaatcgatgc tccgcgtcgt gatccagcga ccgccaacat cctcgcggtg aacgaggagc
     2581 a
//
LOCUS       DASRJB010000720         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3313729, whole genome shotgun sequence.
ACCESSION   DASRJB010000720 DASRJB010000000
VERSION     DASRJB010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313729"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFB80_18990"
     CDS             <1..1100
                     /locus_tag="VFB80_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35927.1"
                     /translation="RICIRIGSSSLKQLDRVAPDWPDLRQPVERQATEPAIEVPPAIY
                     ADAAGQAYKSYEVTHGWTPAGDEESQYLLQIDPTLVKSLREGDELYVAILPEAGEVRS
                     FVITAGREELPRQSAKPAVTVAQLQTRATGGASADPTLTDLTQPEAGSVYGDSQAPVT
                     RPPPGASAPLRSGTQPGWNISDAQDAGNQTGPYAAGQPATPLQPPDTSLLDVPAFDHE
                     QFTGRKTGGGTRVGTTAAGSGAAAVKKPSPRYPSQQPSTSSRSNVRGQDRRIAQENDW
                     QVEEPEARLASRTTAAPSSMMDDSPRRGTSTRGAESAADKEPQFPVLPFTLSLFALFL
                     SLGGNLYLAWTAAEFYSRYKLAVERLRSAAR"
     gene            complement(1138..>3089)
                     /locus_tag="VFB80_18995"
     CDS             complement(1138..>3089)
                     /locus_tag="VFB80_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN35928.1"
                     /translation="ALITCGGCGAAGPAVDGRADRVAAAKTTAPAEIAQRRYPASPAP
                     ASSLSAETDAAGATASADVAEGTGPGQGGDKHAYLKENDFHLVSDSPLSTFSVDVDTA
                     SYSKVRAYLLTHRTLPPPDAVRIEELVNYFDYDYSPPRDDHPFAVHLETAACPWQPVH
                     RLVRCGIQGKLIDQHRPASNLVFLIDVSGSMDAPNKLPLVKQGLQMLVRQLEENDRVA
                     IVVYAGAAGLVLPSTSGQDQPRILSALEDLRAGGSTNGGQGILLAYRIAQENFLQGGT
                     NRVILCSDGDFNVGVTSTGDLVRLAIEKSRAGIYLSVLGFGFGNHNDALLEELADKAD
                     GNYAFIDTQAEARKVLVEQMSGTLVTIAKDVKLQVEFNPAQVAAYRLIGYENRLLADR
                     DFNDDAKDAGEIGAGHRVTALYEIVPIGVPSPSPQVDPLKYQPPAKRDGGSDSDAASV
                     PAAVAEELLTLKLRYQPPHGGASRLMAFPLKDSGQKFGQATSDFQFAAAVASFGMLLR
                     GSRHRGDTSYAAVLETAQASRGKDEHGYRAEFLELVQSAQELANAKGSERSVPQSWTE
                     GRPAPSRETGYLAPPAVGPPKISASQQSPPGWSGDPVKMLAFGTFLGVGLAVIVLTAT
                     AALVNWISQRRFLRGMQPSRKVPIA"
BASE COUNT          545 a         1008 c         1006 g          530 t
ORIGIN      
        1 atcggatctg cattcgcatc ggcagcagca gcctcaagca gctcgaccgc gtcgcacccg
       61 actggcccga tttgcgtcag cccgttgagc gtcaggcgac cgagcccgcg atcgaagtgc
      121 cgccggcgat ctatgccgat gcggcgggac aggcatacaa gtcgtatgaa gtgacgcacg
      181 gctggacgcc cgctggcgac gaggaatcgc agtatctctt gcagatcgac ccgacgctgg
      241 tcaagtcgct acgtgaaggg gacgagcttt acgtggcgat ccttcccgaa gctggggagg
      301 tgcgatcgtt cgtcatcacg gccggccgag aggaactgcc gcgccaatcg gccaagccgg
      361 cggtcacggt ggcgcagctg caaacgcgag ccaccggtgg cgcatccgcc gatcccacgc
      421 tgaccgacct gacacagccg gaagcgggct cggtctatgg cgatagtcag gcgcccgtga
      481 caaggccgcc gcccggtgcg tccgcgcccc tgcggtcggg tacacagccg ggctggaaca
      541 tttccgatgc ccaggatgct ggcaatcaaa ccggtcccta tgcggccggc cagccggcca
      601 cgccccttca gccgcccgat acgtcgctgt tggacgtgcc ggccttcgat cacgaacagt
      661 tcacggggcg caagacaggc ggcggcacac gcgtgggtac gacggcggcg gggagtggcg
      721 cagcggccgt caaaaaacct tccccacgtt acccttcgca gcagccttcg acctcatcgc
      781 ggtcaaacgt gcgcggccag gaccgccgca tcgcccagga gaacgattgg caagttgagg
      841 agccggaagc gcgcctggcg tcgcgaacga ccgccgcgcc atcgagcatg atggacgatt
      901 ctcctcgccg cggcacaagc acgcgcggtg ccgaaagtgc cgccgacaag gaaccgcagt
      961 tccccgtcct gccgttcacc ctcagtttgt ttgcgctttt cttgtcgctg ggcggcaatc
     1021 tgtatctcgc gtggacggcg gccgagtttt acagccggta taagctggcc gtggagcggc
     1081 tgcggagcgc agctcgatga gctggccggt ttccgcgcgg gcctcgccaa aggctactta
     1141 cgcgatcggc actttcctgg acggttgcat gcctcgcaag aagcggcgct gagaaatcca
     1201 gttgacgagc gcggctgtcg ccgtcaggac gatcactgcc aggcccacgc ccagaaacgt
     1261 accgaaggcc agcatcttaa ccggatcccc cgaccagccg ggtggtgact gctgcgacgc
     1321 cgaaattttg ggcggcccca cggcaggggg cgcgaggtat ccggtttccc gcgacggcgc
     1381 gggccgtcct tcggtccagc tctgcggcac gctgcgctcg ctgccttttg cgtttgccag
     1441 ctcctgtgcc gactgcacaa gctcaagaaa ctcggctcga tagccgtgct cgtccttccc
     1501 gcgggacgcc tgcgccgtct ccagcacggc ggcataggag gtgtccccgc ggtgccgcga
     1561 accgcgcagc agcatgccaa acgaagccac cgcagcggca aattgaaagt cgctcgttgc
     1621 ctggccgaat ttctggccgc tgtctttgag cgggaatgcc atgagtctgc tggcgcctcc
     1681 gtgcggcggc tggtagcgca gcttgagcgt cagcagctcc tcggcaacgg ccgctgggac
     1741 cgacgcggcg tcactgtcgc tgccgccgtc ccgcttagcc ggcggctggt acttgagcgg
     1801 atcgacctgc ggcgacggcg atggcacgcc gattggcaca atttcataga gggccgtcac
     1861 ccggtggccc gccccaatct cccccgcgtc cttggcgtcg tcattgaaat cgcggtcggc
     1921 cagcaggcgg ttctcatagc caatcagccg atacgcggca acttgcgctg gattgaattc
     1981 cacttgcagc ttgacgtcct tggcgatcgt caccagcgtg cccgacatct gctcgaccaa
     2041 caccttgcgc gcttcggcct gcgtgtcgat gaaggcgtag ttgccgtccg ccttgtcggc
     2101 aagttcttca agcagggcgt cgttatggtt gccgaagcca aagcccagga ccgagagata
     2161 gatgccggcc cgcgattttt caatcgccag ccggaccagg tcgccggtgc tagtgacgcc
     2221 gacgttgaag tcgccgtcgc tgcacaggat gacgcgattg gtgccccctt gtagaaaatt
     2281 ctcctgggcg atgcgatagg cgagcaaaat cccctgcccg ccgttggtcg aaccgccggc
     2341 gcgcaggtcc tcgagcgccg acaagattcg cggttgatcc tgcccgctcg tcgaagggag
     2401 gaccaggccg gccgcccccg catagaccac gattgccacg cgatcatttt cctcaagctg
     2461 acgcacgagc atctgaagcc cctgcttcac cagcggcaac ttgttcggtg cgtccatcga
     2521 gccggagacg tcgatcaaga agaccaggtt gctcgcaggg cgatgctggt cgatcagttt
     2581 tccctgaatg ccgcagcgca ccagacgatg caccggctgc cacgggcagg cggccgtttc
     2641 cagatggacc gcaaaaggat ggtcgtcgcg ggggggcgag taatcgtagt cgaagtagtt
     2701 gacgagctct tcgatacgga cggcatcggg aggcggaagc gtgcgatggg tcagcagata
     2761 ggcacgcacc ttggaataag aggccgtgtc cacatcgacg gagaacgtgg aaaggggcga
     2821 gtcgctgacg aggtgaaagt cgttctcctt gagatacgcg tgcttgtcgc ctccctgacc
     2881 gggaccagtt ccttcggcaa catctgcgga ggccgtggcg cctgcggcgt cggtctccgc
     2941 ggaaagggag ctggcaggcg ctggcgatgc cggatagcgc cgctgggcga tctctgcggg
     3001 tgcggtcgtc ttagccgcgg ctactcggtc ggcccgccca tccactgcgg gtccggcggc
     3061 gccgcaaccg ccgcaagtga tcaacgcaa
//
LOCUS       DASRJB010000721         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1059690, whole genome shotgun sequence.
ACCESSION   DASRJB010000721 DASRJB010000000
VERSION     DASRJB010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059690"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFB80_19000"
     CDS             complement(<1..468)
                     /locus_tag="VFB80_19000"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35929.1"
                     /translation="MRITDVEPILLRGEQPYAATAGSAEATDNGDWQLLVKVTTDEGL
                     TGWSDVETLASAAVACINGPGMGVLGFRSLKDLLIGKDPLGVLERLWHELYIGTSYYG
                     RRGVALHCISAIDNCLWSIRAQAAGVPLGQLLGGRSCERVTAYASTLFRETPEA"
     gene            complement(573..1418)
                     /locus_tag="VFB80_19005"
     CDS             complement(573..1418)
                     /locus_tag="VFB80_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN35930.1"
                     /translation="MIIDVHSHAWRFPEDFSDDFRRQARRARAGQEVDLAVRLEDYAA
                     HCQEPVRTIVFGGKARLSGLWVNDSYVAQYVAAAPDKLIGFLSVDPTQEGWQDDLRYG
                     HQELKLRGIKLLPMYAGFRPDEPRLDPLWRYAQRHRLPVLLHTGTTFVAQAPLELTLP
                     RHLDAVAARFPEVRIIMAHLGHPYEGECVVTARKHAHVFADISALHYRPWQFYNSLML
                     VQEYGIWDKILFGTDYPFTNVAATLAGLRSLNGMLEGTSLPRLNEEAIERMIYRESLG
                     LLGIE"
     gene            1466..2806
                     /locus_tag="VFB80_19010"
     CDS             1466..2806
                     /locus_tag="VFB80_19010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35931.1"
                     /translation="MSGKLSESLPASPAPTAVRYGVLLFLCSLALLLYIDRVCIGQAA
                     QVICRELKLSKTQFGFVNIAFTIAYCLFEVHTGNWGDRFGSRRVIARIVLWWSAFTAL
                     TGAAMGLWSLIAFRFLFGAGEAGAFPNAARVVTRWFPPHERGFARGAITTTSQLGGAI
                     TPPLAAWLIVLAGWRWTFAIFGLLGVGWAAAFYWWFRDEPSEHSAVSPTELAHIGSTE
                     AAGGKGHPPIPWRLVASSPNVWLLGTIMSVGAALFYLLFQWFPTYLKEVHDLAEQQSG
                     WYTGLVIGGGAAGCVAGGWLSDLVLRRTSDRRWPRRSIGCGMLGLSAFSMFCFGYCEQ
                     AWSATLACAIALFCLQVSIPTWWTVVAEISGEHGAAMFGLMNGLGGVGILIMNLLVGP
                     IVEARQASGILKIEVWQPVFAGVAIALSAGAICWLGVDATRSIVAGEPPPGPAE"
     gene            complement(2813..>3943)
                     /locus_tag="VFB80_19015"
     CDS             complement(2813..>3943)
                     /locus_tag="VFB80_19015"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35932.1"
                     /translation="DLIPSAEVFYAVKANSSRALLAHLAQLGAGFDVASAGELHLVSH
                     VLGIPGQRIVFSNPIKNQDALNVIARARPRVIVVASLDGLRQLDQLPVADSSYRPVLL
                     VRMPVRGSNLNKYGSDEEVIPILRAADASGRFSKLGLTFHVGTQSTSSHKFQEALQKC
                     ARTIERLAGKVKLDVIDAGGGFPDQRAASNAAVPYRSILTDVASYLTRHLPARTEVFV
                     EPGRALVADAGVIVTQIVARTETRSRSGGTRCLQIDDSLYRNLLGQWHDGREWDVHPF
                     TLDENAPLSDELSDFVITGGSCDGIDQLERKGKKGHRLPKDISVGTYGLIDCAGAYTT
                     STATCFNGHENSDVVLFSQSGGQLHSHVERANRPWEDLSAAQ"
BASE COUNT          611 a         1282 c         1310 g          740 t
ORIGIN      
        1 cgcctccggc gtctcgcgaa agagcgtcga ggcataggct gtcactcgct cgcagctccg
       61 gccgcccaag agttggccca gcggcacacc cgcggcctgc gcccggatcg accagaggca
      121 gttgtcgatc gccgaaatgc agtgcagggc cacgccgcgg cggccgtaat atgacgtgcc
      181 gatgtaaagc tcgtgccaca gccgctcgag gacgccgagc gggtccttgc cgatgagcaa
      241 atccttgagc gagcgaaacc cgagcacgcc cattcccggg ccattgatgc aggcgaccgc
      301 cgcggaggct agcgtttcga cgtcggacca gccggtcagt ccctcgtcgg tcgttacttt
      361 cacgaggagc tgccagtcgc cgttgtcggt cgcctcggcg ctgcctgccg tggcggcata
      421 cggctgctcg ccgcgaagca ggatcggctc gacgtcggtg attcgcatgg tttgtgggct
      481 tgcctcgttt aacccggagc cggaggcgac ggggttttcc ctagccgccg tcgcctccgg
      541 ctccgggtta aacgaccagt cggcgcattg gattactcga tccccaacag gcccaacgac
      601 tctcggtaaa tcatccgctc gatcgcctcc tcattcagtc gagggagcga ggtgccctcg
      661 agcatcccgt tgaggctgcg caggccggcg agcgtggcgg cgacgttggt gaagggatag
      721 tcggtgccga acaggatctt gtcccagatg ccgtattcct ggacgagcat caggctgttg
      781 taaaactgcc aggggcgata gtgcagggcg ctgatgtcgg cgaagacgtg cgcgtgcttg
      841 cgggcggtga cgacgcactc gccttcgtac gggtggccca ggtgggccat gatgatccgc
      901 acttcgggaa agcgggccgc cacggcgtcc aggtgccgcg gcagcgtcag ctccagcggc
      961 gcttgcgcga cgaacgtcgt cccggtgtgc agcagcacgg gcaggcggtg ccgctgggca
     1021 tagcgccaca gcggatcgag ccgcggctcg tcggggcgga agccggcgta catcggcagc
     1081 agcttgatgc cgcggagctt cagttcctgg tgaccatagc gcaggtcgtc ttgccagccc
     1141 tcctgcgtcg gatcgaccga aagaaagcca atcagcttgt ccggcgcggc cgcgacgtat
     1201 tgggcaacgt aggaatcgtt gacccacagg ccgctgagcc gggccttgcc gccgaagacg
     1261 atcgtccgca ccggctcttg gcagtgggcc gcgtagtctt ccagccgcac agccaggtcg
     1321 acctcctgcc cggcccgcgc ccggcgggcc tgccggcgga aatcgtccga aaaatcttcg
     1381 ggaaagcgcc aggcgtggct gtgaacgtcg atgatcatgg gcgtatgcta ggggcctgac
     1441 aacactgtag ccggcaaatc gcgcgatgtc agggaagctc tccgaatcac tgcccgcatc
     1501 gcccgcgccc accgccgtcc gctacggcgt gctcttgttt ctctgctcgc tggcgctgct
     1561 cctgtacatc gatcgcgtct gcatcggcca ggcggcccag gtgatttgcc gtgaattgaa
     1621 gctctcgaag acgcaattcg gcttcgtgaa tatcgccttc acgatcgcct actgcctgtt
     1681 tgaagtgcat acggggaact ggggcgaccg ctttggctcg cggcgggtca tcgcgcggat
     1741 cgtgctgtgg tggtcggcgt tcacggcgct caccggagcc gcgatgggcc tgtggagcct
     1801 gattgcattc cgctttttgt tcggggccgg cgaggcggga gcctttccga acgcggcccg
     1861 cgtcgtgacg cgctggtttc cgccgcacga gcgcggtttc gctcgcggcg cgatcaccac
     1921 cacctcgcag ctgggtggcg cgatcacgcc cccgctggcc gcctggctga tcgtgctcgc
     1981 cggctggcga tggacgttcg ccatttttgg cctgctgggc gtcggctggg ccgcggcctt
     2041 ttattggtgg ttccgcgacg agcccagcga acattcggcc gtgtctccca ccgagttggc
     2101 gcacatcggc tccaccgaag ccgcgggcgg aaagggccat ccgccgatcc cctggcgcct
     2161 ggtcgccagc agcccgaacg tctggcttct cggcacgatc atgtcggtcg gcgcggcgct
     2221 cttctacttg ttgttccaat ggtttcccac ctacctcaag gaagtgcacg acctggccga
     2281 gcagcagtcg ggctggtaca ccggactggt catcggcgga ggggccgcgg gctgcgttgc
     2341 gggcgggtgg ctgtcggacc tggtgctgcg ccgcaccagc gaccgccgct ggccgcgccg
     2401 ctcgatcggc tgcggcatgc tgggcctgtc cgccttctcg atgttctgct tcggctattg
     2461 cgagcaggcc tggtcggcca cgctcgcctg cgcgatcgcc ctgttttgcc tgcaggtctc
     2521 gattccaacg tggtggaccg tcgtggcgga gatcagcggc gagcacggcg cggcgatgtt
     2581 cggcctgatg aacgggctgg gaggcgttgg catcctgatc atgaacctgc tcgtcggtcc
     2641 gatcgttgag gcccggcagg ccagcggcat cctcaagatc gaagtctggc agcccgtgtt
     2701 tgccggcgtg gcgatcgcgc tgtccgcggg ggcgatctgc tggctcggcg tcgatgccac
     2761 gcggtcgatc gtcgccgggg agccgccgcc cggcccggct gaatagcgac ttctattgcg
     2821 ccgctgagag atcttcccac ggccgattgg cacgctctac gtgggagtgc agctgacctc
     2881 cgctctggct aaacaagacc acgtctgaat tctcgtggcc gttgaagcaa gtggcagtgg
     2941 aagtcgtgta ggcgccggcg cagtcgatga ggccataagt tccgacgctg atgtccttcg
     3001 gtaaccggtg gcccttcttt ccctttcttt ccagctggtc gattccatca caggaaccgc
     3061 cggtgatgac gaagtcgctt aattcgtccg acagcggcgc gttttcgtcc agcgtgaagg
     3121 gatgcacgtc ccactcgcgg ccgtcgtgcc actgccccag caggtttcga tagagtgagt
     3181 cgtcgatctg caaacatcga gtccctcccg accggctcct cgtctccgtt cgggcaacaa
     3241 tttgcgtcac gatgacgccc gcgtcggcga ccagggctcg ccccggctca acgaatacct
     3301 cggtgcgggc tggcaggtgc ctggtcaggt aggacgcaac gtccgtgagg atgctgcgat
     3361 aaggaaccgc tgcgttcgat gccgcgcgct ggtccggaaa gcctccgccg gcgtcgatga
     3421 cgtccagctt cactttcccg gccaaccgct cgatcgtgcg ggcgcacttc tgaagcgcct
     3481 cctggaactt gtgcgaggaa gtgctctgcg tcccgacgtg gaacgtcagc cccaatttcg
     3541 agaatcgccc tgaagcgtcc gctgcgcgca agatcgggat gacttcctcg tccgatccgt
     3601 acttgttcag attcgaaccg cgcacgggca tccggaccag gagcaccggc cggtagctcg
     3661 agtctgccac tggcagttga tccagctggc gcaagccgtc caggctcgcc accacgatca
     3721 ctcgcgggcg ggcgcgggcg atcacattca gcgcgtcctg gttcttgatc gggttggaaa
     3781 aaacgatccg ctggccgggg attcccagga cgtgactcac gaggtgaagc tcgcccgcgg
     3841 aggcgacgtc gaaacccgcc cccagctgcg caaggtgcgc gagcagcgcc ctgctggagt
     3901 tggccttcac cgcgtaaaac acttcggccg acggaatgag gtc
//
LOCUS       DASRJB010000722         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4158823, whole genome shotgun sequence.
ACCESSION   DASRJB010000722 DASRJB010000000
VERSION     DASRJB010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4158823"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..608)
                     /locus_tag="VFB80_19020"
     CDS             complement(63..608)
                     /locus_tag="VFB80_19020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN35933.1"
                     /translation="MNEGNGLADSRVTPELLGRLLDEQGGALALYAAQWSATPDDCVQ
                     EALIELAAQPRVPASPAAWLFRVVRNRAISQFRSARRRERREQLALRLRPRQGEAPAE
                     LASEELAAALESLSDDLREAVVARTWGGLNFEQIAALAGCSTSTAHRRYEAGLAALRE
                     RLEAPCPTNPRQTNSTGSPAR"
     gene            844..>2195
                     /locus_tag="VFB80_19025"
     CDS             844..>2195
                     /locus_tag="VFB80_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN35934.1"
                     /translation="MHGFEDTGAKPLDDPKDNRPWYKLLNGYHWFVLVVAALGWLFDT
                     MDQQLFNLARTPAITELLGTGASKTQIDAYGAYATSIFIVGWATGGLFFGILGDRIGR
                     AKVMLFTILIYSLFTGLSALSVGVWDFALYRFLTGLGVGGEFAVGVALVAEVMPDRAR
                     THALGWLQTLSAVGNITAALINMSLGVAEGQDMLGEFKPWRIMFVIGALPALLAIAIR
                     ARLKEPERWQHAVAEGGGKVKHQAGSLTELFGDPRWRKHALVGLALAIPGVVGLWGIG
                     FFSFDFVRAIFRPIYEQQGLADAAIRSKLTILVGWVSVLQNLGGAVGIYSFKTITNRI
                     GRRATFALAFILAMVSTAGTFWFLNRGTFWLLDGFYGVFFLIPVMGFCQLTLFGGYAI
                     YFPELFPTRLRSTGTSFCYNVGRYVAALGPLTMGGLINSVFGHHGQIGAWRYTGVTMS
                     "
BASE COUNT          338 a          685 c          733 g          439 t
ORIGIN      
        1 gaacatcagc cggtcgcggt cgagctgcgg cgcagccggc ccgaagccgg cgagcgtctg
       61 ctctagcgag ctggggatcc ggtcgaattc gtttggcgtg gattcgttgg gcatggtgcc
      121 tcgagccttt ctcgcagggc ggcgagcccc gcttcgtacc gtcggtgggc ggtgctcgtc
      181 gagcagccgg ccagggcggc gatctgttca aagttcaatc cgccccaggt gcgggcgacc
      241 acggcctcgc gcaagtcgtc gctcagcgac tccagcgcgg cggccagttc ctcggaagcc
      301 agctcggcgg gcgcctcgcc ttgcctgggc cgcagccgca gggccagctg ctcgcgccgc
      361 tcgcggcggc gggcggagcg gaactggctg atcgcccggt tccgcaccac gcgaaacagc
      421 caggccgcgg ggctggcggg cactcgcggc tgcgcggcca gctcgatcag ggcctcctgc
      481 acgcagtcgt cgggcgtggc ggaccactgg gcggcgtaca gcgccagtgc cccgccctgc
      541 tcgtcgagca gtcggccgag caattccggt gtgacgcggc tgtccgccag gccgttaccc
      601 tcgttcacaa gctccccgcg aaagcgagcg actgtccggc gttcagagtg aagacgccgg
      661 ccgcttgcga ttttcccatg gggcggcctc aaaaacgaga ataatcgatc gctgcggcat
      721 ttcgccgcgg ccctcggcag tgcatcgcgg ggcagaggtt tgctaaacct ttgcgagggc
      781 aaaggcgcaa ggtacactgc ggacctgcct tattccgcga tcccaccaca aggcctctcg
      841 ctgatgcacg gtttcgaaga cacaggcgcc aagccgcttg acgatccgaa agacaatcga
      901 ccgtggtaca agctgctgaa cggctatcac tggttcgtgc tcgtcgtggc ggcgctgggg
      961 tggctgttcg acacgatgga tcagcagctc ttcaacctgg cccgcacccc ggcgatcacg
     1021 gagttgttgg gcacgggagc ttccaagacg cagatcgatg cgtacggcgc ctatgcaacg
     1081 tcgattttca tcgtgggttg ggcgaccggc ggtttgttct tcggcatcct gggcgaccgc
     1141 atcggccggg ccaaggtgat gctgttcacg atcctgatct actcgctgtt cacgggcctc
     1201 agcgcccttt cggtcggtgt ctgggacttt gccttgtacc gcttcctgac gggcctgggc
     1261 gtgggaggcg agtttgctgt cggtgtggct ctcgtcgcgg aggtcatgcc ggaccgtgcg
     1321 cgaacgcacg cgctgggctg gctgcaaacg ctctcggctg tcggcaacat cacggccgcc
     1381 ctgatcaaca tgagtctggg tgtcgccgaa ggccaggaca tgctaggcga gttcaagccc
     1441 tggcggatca tgttcgtgat cggtgcactt cccgccttgc tggcgattgc aatccgggcc
     1501 cgcctcaagg agcccgagcg ctggcagcac gctgtggcgg aaggcggggg caaggtgaag
     1561 caccaggccg gttcgctgac ggaactgttt ggcgatccgc gttggcgcaa gcatgcccta
     1621 gttggcttgg cgctggccat ccctggcgtc gtgggcctct ggggaatcgg ctttttcagc
     1681 ttcgacttcg tgcgggccat tttccgcccg atctatgagc aacaaggcct tgcggatgcc
     1741 gccatccgca gcaagctgac gattctcgtc ggctgggtgt cggtgctgca aaacctgggc
     1801 ggcgccgtgg gaatctacag cttcaaaacg atcacgaacc ggattggtcg ccgggcgacg
     1861 tttgccttgg cattcatctt ggccatggtc agcacggcgg gcaccttctg gttcttgaat
     1921 cgcggtacat tctggctgct cgacggcttc tatggggtct tttttctgat tcccgtgatg
     1981 gggttttgtc agctgacgct ctttggcggc tacgcgatct actttcccga attgttcccg
     2041 acacgcctgc ggagcacggg cacttcgttt tgctacaacg tcggacgata cgtagcggca
     2101 ctcgggccgc tgacgatggg cgggctcatc aacagcgtct ttggccatca cggccaaatc
     2161 ggagcgtggc gctataccgg cgttaccatg agcat
//
LOCUS       DASRJB010000723         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3875246, whole genome shotgun sequence.
ACCESSION   DASRJB010000723 DASRJB010000000
VERSION     DASRJB010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875246"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFB80_19030"
     CDS             complement(<1..943)
                     /locus_tag="VFB80_19030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HZN35935.1"
                     /translation="MLSRALCCQIFAALLLAAPLAAEDKLHKRVEEFLVQPHFKTAHI
                     GLLFVDLDTGEVILERSSEKLFAPASTTKLYSTAAALDALGAEYRFHTPVYRRGTVNE
                     TGELEGDLILVASGDLCFGGRTDEKGEIAFVDSDHTYANWMSDSGLTPQDPLAGINDL
                     AKQIAAAGIKKVLGDVLIDDRLFDRSEGSGSGPSTLTPIIVNDNVLDFSIEPAEPGQP
                     AKCAWRPQTALFRVEFDVQTVKENEPLETTIRAQGDGRIVVTGKIPANKSRLVRIYEV
                     PDPAAFARGLLIEALQRSGVEVAAGLAPKHPEGKLPER"
     gene            complement(976..2028)
                     /locus_tag="VFB80_19035"
     CDS             complement(976..2028)
                     /locus_tag="VFB80_19035"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HZN35936.1"
                     /translation="MKHDVGVYGKTKDGQEVSLHTLTNVPGLRVKLISYGATLISVEM
                     LDKQGKSANITLGFGSLDGYLQRHPYFGSTVGRYANRIAGGKFTLDGQACTLAKNNGP
                     NHLHGGLKAFDAAVWKAEPVTGPGIVGVKFSHESPDGDEGYPGKLNVTTTYTLNNQNE
                     LRIEYTATTDKPTVVNLTNHAYWNLAGAGSGDVLKHVLTINADKYLPIDATSIPTGVL
                     APVAGTPLDFTTPHAIGERIGELKKEPHQTKGYDHCYVLRGQAGKLELAARVSDPASG
                     RVMEVLTTEPGVQLYCGNFLGGGAGEGGYQQHEGFCLETQHYPDSPNQSDFPTTVLRP
                     GQTYRSTTVHRFSLEK"
     gene            complement(2228..3178)
                     /locus_tag="VFB80_19040"
     CDS             complement(2228..3178)
                     /locus_tag="VFB80_19040"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN35937.1"
                     /translation="MTTAPAGIDRRSLLAAGAAALGTAAVAVQAQEQPRPIILPPAGK
                     PILLSCKLGMIAKKENGKDLSLVERLKLAAQAGFDGVDFDEAGSFTPEQARAAVQESG
                     LFVHNAINHAHWQQRLTSANEAERAKGRANIEHCLRVAHAAGGSGVLIVVGRGGDGPA
                     DEIDERARQEIKKLIPLAASLGQPILFENVWNQMHYDHDAPPEQSPERFVRFIDSFNS
                     PWVGQYYDIGNHWKYGQPREWLKAFGRRAVKLDVKGFSRAKNKFVDITSPDDDLPWKE
                     VQQGLEEIGFTGWTTAEVGGGGLERLTTVRQQMQKAFGLS"
     gene            complement(3213..>3948)
                     /locus_tag="VFB80_19045"
     CDS             complement(3213..>3948)
                     /locus_tag="VFB80_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35938.1"
                     /translation="SESKEAEVQVAAVAALQKLASGEGLVARRAGELLKPKPVTSAAT
                     PPAVPGIPAPVIRGFGFGGGGIRIAAAAGDGVKRISVKDENGVKEIEVVEGDRSIKIN
                     DDPAKGIKLKVTEKKDGKEETKEYEAKNADELKTKHPEAHKLYQQYKSGGAEIRFEAI
                     PGLLPGIGAIPAPPVPAIRRPAIIPDAKEATDRLDKARQQLDEAAARLKKLPETSAIA
                     AAEIKAALEAIEQSKKELEALRAKLP"
BASE COUNT          576 a         1293 c         1298 g          781 t
ORIGIN      
        1 ctcgctcggg gagcttgcct tccgggtgct tgggcgccag cccggctgcg acttcgacgc
       61 cgctccgctg aagcgcttcg atcaagaggc cgcgggcgaa cgccgccggg tcgggcactt
      121 catagatccg caccagccgg ctcttgttgg cgggaatctt gcccgtcacg acgatccggc
      181 cgtccccctg ggcgcgaatg gtggtctcca gcggctcgtt ctccttgacg gtctgcacgt
      241 cgaattcgac gcgaaagagc gctgtctgcg gccgccaggc gcatttcgcc ggctgccccg
      301 gctcggccgg ttcaatcgaa aagtcgagca cgttgtcgtt gacgatgatc ggcgtcagcg
      361 tgctgggtcc gctgccgctg ccttcggagc ggtcaaacag ccggtcgtcg atcagcacgt
      421 cgccgaggac cttcttgatc cccgcggcgg caatttgctt cgccaggtcg ttgatgccgg
      481 cgagcggatc ttgcggcgtc aggccgctgt cgctcatcca gttggcgtag gtgtggtccg
      541 aatcgacgaa ggcaatctca cccttttcgt cggtgcggcc gccgaagcag agatcgccgc
      601 tggccacgag gatcaggtcg ccctccagct cgcccgtctc gttcacggtt ccgcggcggt
      661 agaccggcgt gtgaaaacgg tattcggccc ccaatgcatc aagcgccgca gcggtcgaat
      721 agagcttcgt cgtcgaggcc ggggcaaaca gcttctcgct gctccgctcc aggatcacct
      781 cgcccgtatc cagatcgacg aacaagaggc cgatgtgggc ggtcttgaag tgcggctgca
      841 cgaggaattc ctcgacgcgc ttatggagtt tgtcttcagc cgccagcggg gccgcgagca
      901 aaagagcggc gaaaatctgg cagcataagg cgcgcgaaag cataggcgaa aaacctgaca
      961 aggaactggc cgggcctact tctcgaggga gaaccggtgg accgtcgtcg agcgataggt
     1021 ctggcccggc cgaagcacgg tggtgggaaa gtcggactgg ttgggcgaat cgggatagtg
     1081 ctgcgtctcc aggcagaagc cttcgtgctg ctggtagcct ccttcgcccg cgccgccgcc
     1141 gaggaagttg ccgcagtaaa gctgcacgcc gggctcggtc gtgagcactt ccatgacgcg
     1201 gccgctggcc ggatcgctca cccgggccgc cagctccagc ttgcccgcct ggccccgcag
     1261 cacgtagcag tggtcgtagc ccttggtctg gtgcggctcc ttcttcagct cgccgatccg
     1321 ctcgccgatg gcgtgcggcg tcgtgaaatc cagcggtgtg ccggcgaccg gggcgagcac
     1381 gccggttgga atcgacgtgg cgtcgatcgg caggtacttg tcggcgttga tcgtcagcac
     1441 gtgcttgagg acgtcgccgc tgcccgcacc cgcgaggttc caataggcgt ggttcgtcag
     1501 gttgacgacc gtcggcttgt cggtcgtcgc ggtgtattcg atccgcaact cattctggtt
     1561 gttgagcgtg taagtcgtcg tgacattcaa cttgcccgga taaccttcgt cgccgtccgg
     1621 gctctcgtgg ctgaatttca cgcccacgat gcccggcccg gtgaccggct cggccttcca
     1681 gacggcggcg tcgaacgcct tcagaccgcc atgcaggtgg ttcgggccgt tgttcttcgc
     1741 tagcgtgcag gcctggccgt cgagcgtgaa cttgccgccg gcaatccggt tggcatagcg
     1801 gccgacggtc gagccaaagt agggatgccg ctgcaggtag ccgtcaaggc tgccgaagcc
     1861 gagcgtgatg ttggcggatt tcccctgctt gtcgagcatc tcgacgctga tcaaggtcgc
     1921 gccgtagctg atcaatttca cccgcaaccc gggcacgttg gtcagcgtgt gcagcgaaac
     1981 ctcctgcccg tccttcgtct tgccatagac tccgacgtcg tgcttcatgg tcgcggcgag
     2041 tgttgtcaat tcgggcctgg ggatggtctt tccgtctacg cggcgcggct gtgccctggc
     2101 ttcgcggccg gcgagcgccg cgagcgctag gcacaatgcg attctaatcc cgcgatcact
     2161 cctgcaaatc gggcagcgca tggaagtctc caccagcaag gatgatttgt cgcgtgaggc
     2221 cgcaggctca gctcagcccg aaggccttct gcatttgctg ccgcacggtc gtcagtcgct
     2281 ccaggccccc gccgccgacc tcggccgtcg tccagccggt aaagccgatc tcctccagcc
     2341 cctgctgcac ttccttccag ggcaggtcgt cgtcggggga ggtgatatcg acgaacttgt
     2401 tcttcgcccg gctgaagccc ttcacgtcga gcttcaccgc ccggcggccg aaggccttga
     2461 gccactcccg cggctggccg tatttccaat ggttgccgat gtcgtagtac tggccgaccc
     2521 aggggctgtt gaagctgtcg atgaagcgga cgaaccgctc gggcgactgc tcgggcggag
     2581 cgtcgtggtc gtagtgcatc tggttccaaa cgttctcgaa caggatcggc tgcccgagcg
     2641 atgcggccag cggaatcagc ttcttgatct cttggcgggc ccgctcgtcg atttcgtcgg
     2701 ccgggccgtc gccgccgcgg cccacgacga tcaagacgcc gctgccgccg gcggcatggg
     2761 ccacgcggag gcagtgctcg atgttggccc ggcccttggc ccgctcagct tcattggcgc
     2821 tggtcagccg ctgctgccag tgggcgtggt taatggcgtt gtgcacgaac aggccggatt
     2881 cttgcacggc agcgcgggcc tgctcgggtg tgaacgagcc ggcttcatcg aaatcgacgc
     2941 cgtcaaagcc ggcctgggcc gcgagcttca gccgctccac aagcgagagg tccttgccgt
     3001 tttccttctt ggcgatcatg ccgagcttgc aggagagcag aatcggcttg cccgcgggcg
     3061 gcaagatgat cggccgcggc tgctcctgag cctggaccgc gactgcggct gtccccagcg
     3121 cggccgctcc ggcggcgagc aatgaacggc ggtcgattcc ggcaggcgcg gtcgtcatcg
     3181 gtcggctcct ggtggtgatg tcgtgaacgc ggctagggca gcttggcgcg gagcgcctcc
     3241 agctccttct tcgattgctc gatcgcctcc agcgcggcct tgatctccgc cgcggcaatg
     3301 gcactcgtct ccggcaactt cttcagccga gcagcggcct cgtcgagctg ctggcgggcc
     3361 ttgtcgagcc ggtcggtggc ctccttggcg tccggaataa tcgcgggccg gcggatggcc
     3421 ggcacgggag gcgccgggat cgctccgatg cccggcagga gacccggaat cgcctcgaag
     3481 cggatttccg ccccgccgct cttgtattgc tggtagagct tgtgggcctc cggatgtttg
     3541 gtcttgagct cgtcggcgtt cttggcctcg tattccttag tctcctcctt gccgtccttc
     3601 ttctcggtaa ccttcagctt gatgcccttg gccggatcgt cgttgatctt gatgctgcgg
     3661 tcgccctcca cgacctcgat ctccttgacg ccattctcgt ccttgaccga gatgcgcttg
     3721 acaccgtcgc ctgcggcggc tgcgatgcga atgcctccgc ctccaaagcc aaagccgcga
     3781 atgaccggcg cgggaatgcc gggaacggcc ggcggcgtgg ccgctgaggt gacgggcttg
     3841 ggcttgagca actccccagc gcggcgggcc accagcccct cgccgctggc cagcttctgt
     3901 agcgctgcga ccgccgctac ctggacctcg gcttccttcg actccgac
//
LOCUS       DASRJB010000724         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2177473, whole genome shotgun sequence.
ACCESSION   DASRJB010000724 DASRJB010000000
VERSION     DASRJB010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177473"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFB80_19050"
     CDS             complement(<1..398)
                     /locus_tag="VFB80_19050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN35939.1"
                     /translation="MSEQDHPRPLWAGRHVSVLARGKWEFVTRNTRRPAVGIVAITGE
                     RRIVLVEQFRPPVNARVIELPAGLAGDQAGAEQEPLLAAAQRELLEETGYRAARWTEL
                     AGGYSSPGLTDELVVLFLAEGLTKVAPGGG"
     gene            complement(401..1240)
                     /locus_tag="VFB80_19055"
     CDS             complement(401..1240)
                     /locus_tag="VFB80_19055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HZN35940.1"
                     /translation="MNRTSRAAIAQETVEIANRGWYETPAGKRVDISAAMDRCLSGTA
                     LYRPGELDRLPPRTGHPAGLHAAFEVANETSLAAARRLVVERGCSATMCLNFASAKNA
                     GGGFLGGSQAQEESLARASGLYRSLLTQDEYYAANRACGTCLYTDHMIFSPAVPVFRD
                     DEGQLLEEPYCLDIVTAPAVNKGAILKNEPQRAHDIGPTMRGRIENLLRLAADRGCEH
                     LILGAWGCGVFRNDPEEVASLFAFVLGGEPFRNRFRTIVFAVLDSAPDERTIGPFRRI
                     WGS"
     gene            complement(1241..1693)
                     /locus_tag="VFB80_19060"
     CDS             complement(1241..1693)
                     /locus_tag="VFB80_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HZN35941.1"
                     /translation="MAIRFYSTKDKYGEFSNFARFPFILDGREWPTSEHYFQAQKFQD
                     HAYQEKIRTTASPMIAARLGRSRAVPIRADWEAVKLDVMRAAVRQKFRSHPQLAALLL
                     ETGNEEIVEVTTRDLFWGCGTSGAGKNWLGKILMEVREQLRADQEQSV"
     gene            complement(1703..2299)
                     /gene="pncA"
                     /locus_tag="VFB80_19065"
     CDS             complement(1703..2299)
                     /gene="pncA"
                     /locus_tag="VFB80_19065"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HZN35942.1"
                     /translation="MKALLLIDIQNDFLPGGALAVRDGHEVVPVANRLMADYPLVVAT
                     QDWHPADHRSFASQHSGQQVGQRTELQGLPQILWPDHCVQGTPGADFPPQLDRRGMHH
                     VVRKGTDPAIDSYSGFFDNARRKATGLEDYLRSRGVDEVHILGLATDYCVKATALDAR
                     SLGFATRLITAGCRGVELAAGDCQRAIEEMRAAGVEIA"
     gene            complement(2296..2757)
                     /locus_tag="VFB80_19070"
     CDS             complement(2296..2757)
                     /locus_tag="VFB80_19070"
                     /inference="COORDINATES: protein motif:HMM:NF027223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HZN35943.1"
                     /translation="MKYIEAHDEFDGRGPAVFLAGGITGAENWQALLADSLRELPAAV
                     LNPRRQQFPADDPAAARQQIEWEHRHLRLASLVAFWFPPPTLCPIALFELGACCAAGT
                     PLVVGSHPQYARRFDLQVQLSLRRPDVELVDSLSALAAQIIAHPALRGAYL"
     gene            complement(2765..>3169)
                     /locus_tag="VFB80_19075"
     CDS             complement(2765..>3169)
                     /locus_tag="VFB80_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN35944.1"
                     /translation="YYALVNLAGHHVRASTDARNAAWFPIGELPDLAFDHCRIVDAAL
                     ARLRGKVRYQPLGFELLPERFTLRQLQHLYEIVLGRPLDKRNFRKKVLGMGILKDTRE
                     IEKDVAHRAARLFRFDERAYERLKRRGFLFEL"
BASE COUNT          530 a         1070 c         1033 g          536 t
ORIGIN      
        1 tcgccgccgc cgggtgcgac tttagtcagc ccctcggcga ggaacagcac gaccagttcg
       61 tcggtcagac cgggcgatga gtatccgccg gccagctcgg tccaccgcgc tgcccggtag
      121 cctgtttctt cgagcagttc ccgctgggcc gccgccagca gcggctcctg ctccgcgccc
      181 gcctggtcgc cggccagccc tgccggcagc tcgatcacgc gggcgttcac cggcgggcgg
      241 aactgctcga ccagcacgat ccgccgctcg cccgtgatcg cgacgatgcc cacggccggc
      301 cgccgcgtgt tgcgcgtgac aaactcccat tttccgcggg ccaggaccga cacatggcga
      361 ccggcccaga gcggccgtgg atgatcttgc tcggacatgg ttaactcccc cagatccgcc
      421 gaaacggtcc aatggtccgc tcgtcgggcg cgctatcgag cacggcaaac acgatggttc
      481 ggaagcggtt gcgaaacggc tcgccaccga gcacgaacgc gaatagcgac gccacctcct
      541 ccggatcgtt tcggaaaacg ccgcagcccc atgcgccgag gatgagatgc tcgcaacctc
      601 gatcggccgc caggcggagc agattttcga tgcggccgcg catcgtcggc ccaatgtcat
      661 gcgcccgctg cggttcgttt ttcaggatcg cgcccttatt gacggcgggc gcggtgacga
      721 tgtcgagaca atacggctcc tccagcaact ggccctcgtc gtcgcgaaag accgggacgg
      781 cgggactgaa gatcatatga tccgtgtaca agcacgtgcc gcaggcgcga ttggccgcat
      841 agtattcgtc ctgcgtgagg agcgaccgat acagaccgct cgcgcgtgcg aggctttctt
      901 cctgggcctg gctgccgccc aagaatccgc cgccggcgtt tttcgccgat gcaaagttca
      961 agcacatcgt cgcggagcag ccgcgttcca caacgaggcg ccgcgccgcc gccagcgagg
     1021 tttcattggc gacttcaaac gcggcgtgca agcctgcggg atgcccggtc cgcggcggga
     1081 gtctgtccag ctcgccgggc cggtacagcg cggttccgct caggcaccgg tccatcgcgg
     1141 cagagatatc cacgcgctta cctgccggcg tctcgtacca gccgcgattg gcgatttcga
     1201 cggtttcctg cgcgatcgcc gcgcgcgacg tgcggttcat tcagacactt tgctcctggt
     1261 ccgcgcgcag ctgctcgcgg acttccatca aaatcttgcc gagccaattc ttgccggcgc
     1321 cgctggtccc gcagccccaa aagaggtcgc gcgtggtcac ttcgacgatc tcttcattgc
     1381 cggtctcgag caacagcgcg gcgagctgcg ggtgcgaacg gaacttctgg cggacggcgg
     1441 cccgcatgac gtcgagtttg accgcttccc aatcggcgcg aatcgggacc gcccggctgc
     1501 gtcccaggcg agcggcaatc atcgggctgg ccgtggtgcg gattttctcc tggtacgcat
     1561 ggtcctggaa cttttgagcc tgaaagtagt gctcgctcgt cggccactcg cgtccatcca
     1621 ggatgaacgg gaagcgggcg aaattcgaga actcgccgta cttgtctttc gtcgaataga
     1681 accggatcgc catggtttgc tcctacgcaa tctccacgcc cgccgctcgc atttcctcga
     1741 tcgcccgctg gcagtcgccg gcggccagct cgacgccgcg gcagccggcg gtgatcagcc
     1801 gcgtggcgaa gccgaggctt ctggcatcga gggccgtcgc cttgacgcag taatcggtcg
     1861 ccaggcccag gatgtggacc tcatccacgc cgcggctgcg aaggtaatcc tccagccccg
     1921 tggccttgcg gcgggcgttg tcgaaaaagc cgctgtagct gtcgatcgcc ggatcggttc
     1981 ccttacgcac gacgtggtgc atgccgcgcc ggtccaattg cggcgggaag tccgcgccgg
     2041 gagttccctg aacgcagtgg tcgggccaga ggatttgcgg cagcccctgc agctcggtcc
     2101 gctggccgac ctgctggccc gagtgctggc tggcgaacga ccggtgatcc gccggatgcc
     2161 agtcctgggt cgcgacgacc agcggatagt cggccatcag gcggttcgcc accggcacga
     2221 cttcgtggcc gtcgcggaca gccagcgcgc cgccgggcag aaaatcgttc tgaatgtcga
     2281 tcaacagcag ggctttcata ggtacgcgcc tcgcagggcc ggatgggcga tgatctgtgc
     2341 ggcaagcgcg gacagcgaat cgactagctc cacgtcgggc cggcgcagcg acaactgcac
     2401 ttgcaagtcg aatcgcctcg cgtattgcgg gtgcgagccg acaaccagcg gcgttcccgc
     2461 tgcacagcac gcgcccaatt cgaatagcgc gatcgggcac agcgtcggcg gcggaaacca
     2521 gaacgcgacc agcgaggcca aacgcaagtg ccgatgctcc cactcgatct gctggcgtgc
     2581 tgcagcggga tcgtcggcgg gaaactgctg gcggcgcgga ttaaggactg ccgccggcaa
     2641 ctcgcgcagc gaatcggcca acagcgcctg ccagttttcc gccccggtga taccaccggc
     2701 caggaacacc gccgggccgc ggccgtcgaa ttcgtcgtga gcttcgatgt atttcatggt
     2761 gctctcacag ctcaaacagg aacccccgcc gcttcagccg ctcgtaggcc cgctcgtcga
     2821 agcggaacag ccgggcggcg cggtgggcga cgtccttctc gatctcgcgg gtgtccttga
     2881 ggatgcccat gccgagcacc ttcttgcgaa agttccgctt gtcgagcggc cggcccagga
     2941 cgatctcgta caggtgctgc agctgccgca gggtgaaccg ttccggcagc aactcgaacc
     3001 ccagcggctg atagcggacc ttgccgcgga gccgggccag tgcagcgtca acgatgcggc
     3061 agtggtcgaa ggccaggtcg ggcagctcgc cgataggaaa ccaggcggca ttgcgggcat
     3121 cggtgctggc ccgcacgtgg tgcccggcca ggttgaccag ggcgtaata
//
LOCUS       DASRJB010000725         6478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_776058, whole genome shotgun sequence.
ACCESSION   DASRJB010000725 DASRJB010000000
VERSION     DASRJB010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776058"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFB80_19080"
     CDS             <1..428
                     /locus_tag="VFB80_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN35945.1"
                     /translation="EANKIAAIQQEFVSFCTDVGPAGGSLIAGHPRAFGAFPRLLSRY
                     VRDQGAISLERAVAQASAAGANNVLALDRGRIAEGLAADIIVLDYLRLTDKATFTQPA
                     ALSEGMQYVIVNGVLVLDGGRQTDARPGRVLRGPGYRSQ"
     gene            complement(429..2549)
                     /locus_tag="VFB80_19085"
     CDS             complement(429..2549)
                     /locus_tag="VFB80_19085"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN35946.1"
                     /translation="MWIERISRAVASGYGLNEVASVLLIASAAGLAAAAEPIAIQPRL
                     VHLRNDPQREWSSFPEKPEAVFLEAKFAATANVGEFALRLRQHDVKQAWRVVLNGKPL
                     GELVRDEADLVIYFPIKPESLVDGENTLRIESPARGPQNADDIRVGEIRLEPRPVAEA
                     LSEATLEIEVIDEVAKTPIPARITIADANGSRQTTAAASSDWLAVRPGVVYTSDGHAR
                     FGVPAGKYTIYAGRGFEYSVAEVETTLTPGQTAQHTLKIRREVDTTGYVACDTHIHTL
                     THSGHGDATLAERMITLAGEGIELPIATDHNVHVDYEKLAQALSVRQHFTPVIGNEVT
                     TPTGHFNIFPVSATASVPDYKAKEWSATFDGIFATPDVKVAILNHARDLHSGVRPFGP
                     ALFNAAAGENVAGWPMRFNAMEVINSGATQTDPLRLPRDWMALLNRGYKVTPVGSSDS
                     HDVARYIVGQGRTYVRCEDRDPANLDVESAVDNFLDGRVLVSYGLLTIVYWAGVEGDS
                     QDPGMMTVSLGVLGPTWTTANRLQLYANGELIREEPITPSKDGERAPGVKYFGSWKVP
                     RPKHDVRIVAIALGKGIDGPFWTTAKPYQPTSPDWQPYVLGISGATWFDGDGDGRKSS
                     AREYAERAVAAAAGDPAQLVAELAKYDAAVATQAAHLYRIAGGSLTAEPLLESLKSAA
                     PAVVNGFRAYREAWRENELAQTQR"
     gene            2636..4129
                     /locus_tag="VFB80_19090"
     CDS             2636..4129
                     /locus_tag="VFB80_19090"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZN35947.1"
                     /translation="MKTLAAACLLLLFLAPVAAQPLPAAKITPPFVTVDLNIGESQEV
                     TLDGKPVAVKLLDLKETNDTLRNAVRRAEVTVEVAGEKVTLVSANYNLPVTVGGVQID
                     CPITRGYTKNSTKTTGGLAPWGLVKDARLRLWPAGSPWLNPGTFQYPAKQAWFASQTQ
                     MANEPTYVDGGERPGMGSVYYHYGLDIGGAEGLIDVVAATDALVVSVGKEVLPGYDGT
                     PVSPRYDVLYLLDDRGWFYRYSHLHTIAADLKPGQTVRRGQALGILGKEGGSGGWSHL
                     HFDISGKQPSGQWGIIDGYAFLWEAHRKEKQPQLIAVARPHHFVAVGEEVQLDGSRSW
                     SASGPITKYFWTCTDGQTADGPQFSRTYGKPGVYSEVLEVTDPQGRRHYDFAVVNVID
                     RAHPEQLPPSIHASFAPTEGIKAGEAIKFLVRTFRTTDGEETWDFGDGSPPVVVHSDG
                     NAVQLAKDGYAQTVHKYAKPGTYLVRVQRTNKLGFTATARLKVTVGE"
     gene            complement(4137..4979)
                     /locus_tag="VFB80_19095"
     CDS             complement(4137..4979)
                     /locus_tag="VFB80_19095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN35948.1"
                     /translation="MAVRPSSLVLTTLGAVLLTAAAVRAQSAPTWFEPMKEVRSRFTG
                     TPGTLALFGDSITVSLAFWAPLQGEPKQMPPEMAAALRLVRGHQQPQCWREWRGPENG
                     NEGGMTIRWARENVESWLTKLNPEVAVILFGSNDLSQLDVQEYEQKTAEVVDRCLKNG
                     TVVLLTTVPPRSGILEKSKQFGDAVRRVAKSKQVLLIDYQAEILKRRPDDWDGALAKF
                     KDTPGSEYEVPTLICRDGVHPSNPQAHQDFSQESLSRNGYLLRNYLTLLAYADVIDRV
                     LAKK"
     gene            5107..>6478
                     /locus_tag="VFB80_19100"
     CDS             5107..>6478
                     /locus_tag="VFB80_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN35949.1"
                     /translation="MNLRALFFVVVSAACSAALPAAEPPRWNILFCFADDWGRYASCY
                     AAVDGRPSLNQVVRTPNVDRVAREGVLFRNAFVNAPSCTPCRSSLLSGRYFFNCGRGA
                     ILQGAIWDSAIPSFPLLLRDAGYQIGKSYKVWSPGTPADAPFGGQQYAYEQAGRLPNN
                     FSEEATKLIEAGATVAEAREKILAQVRGNFDAFLDKRERGKPWLYWFGSTTTHRVWIK
                     GSGKKLWGIEPQSLAGKLPKFLPDVPEVREDVADYLGECQAFDAEVGVLLKRLEEVGE
                     LDRTLIVLSGDHGMPGVPAGKCNLYDHGVSVALAIRVPGGKGGRIVDDFVRLPDLAPT
                     FMEVGGATPPAGLYGKSLVPLLTSEKSGQIDSERNWVVTGRERHVGNAREENLPYPMR
                     ALRTPQFVYIRNFAADRWPMGSPKEAASVPPPAGEAWENNTFAGFADMDGSPTKAWLI
                     AHQKDTH"
BASE COUNT         1094 a         2150 c         2094 g         1140 t
ORIGIN      
        1 ccgaggccaa taaaattgcc gccatccagc aggagttcgt ctcgttctgc accgatgtcg
       61 gacccgccgg cggctcgctc atcgccggac atccgcgagc tttcggagct ttcccgcggc
      121 tgctgtcgcg ctatgtccgc gaccagggcg cgatctcgct ggagcgggcc gtcgcccagg
      181 ccagcgccgc gggcgcgaac aacgtgctgg cgctcgatcg cggccgcatc gccgagggcc
      241 tcgccgcgga catcatcgtg ctggactacc tgcggctgac cgacaaggcg accttcacgc
      301 agccggcggc gctatcggaa ggcatgcaat acgtgatcgt caacggcgtc ctggtgctcg
      361 acggcggcag gcagacggac gctcgccccg gccgcgtgct gcgcggaccg ggatatcggt
      421 cccaatgact agcgctgcgt ttgagccagc tcattctcgc gccaggcttc gcgataggct
      481 cgaaagccgt tcacaactgc tggtgcagcc gacttcagcg actctaagag cggctcggca
      541 gtcagggagc cgcccgcgat gcggtacagg tgcgccgcct gagtcgccac cgcagcgtcg
      601 tatttcgcca gctccgccac gagctgcgcc ggatcgccgg ctgcggcagc cactgctcgt
      661 tcggcatatt cccgtgcgga tgacttgcga ccgtcgccgt caccgtcgaa ccaggtcgca
      721 ccgctgattc caagtacata gggttgccaa tcgggcgatg tcggctgata gggcttcgcc
      781 gtcgtccaaa atggaccgtc gattcccttg cccagcgcga ttgccacgat gcgcacgtcg
      841 tgcttggggc gggggacttt ccagctgcca aaatacttga cgccgggcgc tcgctcaccg
      901 tccttggaag gcgtgatcgg ctcttcgcga atcaattccc cattcgcata gagctggagg
      961 cggttcgcgg tcgtccacgt cgggcccaag acacccaagc tcacggtcat catgcccggg
     1021 tcttgtgagt cgccctcgac gccggcccaa tagacgatcg tcaggagccc gtagctgacc
     1081 aacacgcgcc cgtcgaggaa attatcaacg gcactctcca cgtcgaggtt cgccgggtcg
     1141 cggtcttcgc agcgaacgta ggtccgcccc tgcccgacga tgtaccgggc cacgtcgtgc
     1201 gagtcgctgc tgccgacggg cgtcactttg tagccgcggt tgagcagtgc catccagtcc
     1261 cgcggcaggc gcagcgggtc ggtctgcgtc gcgccgctgt tgatgacctc catggcattg
     1321 aacctcatcg gccagccggc gacgttctca cccgccgcgg cgttgaacag cgccgggccg
     1381 aacggccgca cgccgctgtg caggtcgcgg gcgtgattca ggatggcgac cttgacgtct
     1441 ggcgtggcga agatgccgtc gaaggtcgcg gaccattcct tcgccttgta atccggcacg
     1501 ctggccgtcg ccgagaccgg gaaaatgttg aaatggccag tcggcgtcgt gacttcgttg
     1561 ccgatcacgg gcgtgaagtg ctgccgcacg ctcagcgcct gggcgagttt ctcgtaatcg
     1621 acgtgcacgt tgtggtcggt ggcgatcggc agctcgatcc cttcgccggc cagtgtgatc
     1681 atccgctcgg caagcgtggc gtcgccgtgg ccggagtgag tcagcgtgtg aatgtgcgtg
     1741 tcgcaggcga cgtagccggt cgtatcgact tcgcggcgga ttttcagggt gtgctgcgcg
     1801 gtttggccgg gcgtcagcgt cgtctcgacc tcggccacgg agtactcgaa gccgcgcccg
     1861 gcgtagatcg tgtactttcc ggccggcacg ccaaagcgag catggccgtc ggaggtgtag
     1921 acgactcccg gccgcacggc gagccagtcg ctgctggcgg cggcggtcgt ctgccgcgag
     1981 ccattggcat cggcgatcgt gattcgcgcc ggaatgggcg tttttgcgac ctcgtcgatg
     2041 acctcgattt ccagcgtcgc ctcggaaagc gcttcggcca ccggccgcgg ctccagccgg
     2101 atttcgccca cgcggatgtc gtcggcgttc tgcggcccgc gggcaggcga ctcgatccgc
     2161 agcgtattct cgccatccac gagcgactcc ggcttgatgg gaaaatagat caccaggtcc
     2221 gcctcatcgc ggaccagttc gcccagcggc ttgccattca gcaccacgcg ccaggcctgc
     2281 ttcacgtcgt gctgccgcaa gcgcagcgca aactcgccca cgttggccgt cgcagcaaac
     2341 ttcgcttcca gaaataccgc ctcgggcttc tccgggaacg acgaccactc tcgctgcggg
     2401 tcgttcctca aatggaccag ccgcggctga attgcgatcg gttcggccgc ggcagccagc
     2461 cccgccgccg acgcgatcag cagcacggat gcgacttcgt ttagtccgta gccggaggcg
     2521 acggcgcgcg aaatacgttc gatccacatg gcttagccac ctcgcaagtt tcgttggcct
     2581 tgcttcgact gtataacagc cggctgacgc ccgcgacctc tgccccggcc aatctatgaa
     2641 aacccttgcc gctgcgtgct tgctgctcct cttcctcgct ccggtcgccg cccagccgct
     2701 gccggcggcg aaaatcacgc cgccctttgt caccgtcgat ctcaacatcg gcgagtcgca
     2761 ggaagtaacg ctcgacggca agccggtcgc ggtgaaactg ctggacctga aggagacgaa
     2821 cgatacgctg cgcaacgccg tgcggcgcgc ggaagtgacg gtggaagtcg cgggagagaa
     2881 agtcacgctc gtctcggcca actacaacct gcccgtgacc gtcggcggcg tgcagatcga
     2941 ctgcccgatc acccgcggct acaccaaaaa ctcgacgaaa accacgggcg gcctcgcacc
     3001 ctggggactg gtcaaggacg cccgtctgcg gctgtggccg gccggctcgc cctggctcaa
     3061 tccgggcacg tttcagtacc ccgcgaagca ggcctggttc gccagccaga cgcagatggc
     3121 caacgagccg acgtacgtcg atggcggcga gcggccgggg atgggcagcg tctattacca
     3181 ctacggtctc gacatcggcg gcgcggaggg gctgatcgac gtcgtcgccg cgaccgacgc
     3241 gctcgtcgtt tcggtcggca aggaagtgct gcccggatac gatggtacgc ccgtctcgcc
     3301 gcgctatgac gtgctctacc tgctcgacga tcgcggctgg ttctatcgtt acagccacct
     3361 gcatacgatc gccgccgact tgaaacccgg gcaaaccgtc cgccgtggcc aggccctcgg
     3421 catcctgggc aaagaaggcg gcagcggcgg ctggtcgcat ttgcacttcg atatcagcgg
     3481 caagcagccg tcgggccagt ggggcatcat cgacggctac gcgttcctgt gggaggccca
     3541 ccggaaggag aagcagccgc agctgatcgc cgtcgcccgg ccgcaccact ttgtcgcggt
     3601 cggtgaggaa gtgcagctcg acggcagccg cagctggtcg gcctccgggc cgatcacgaa
     3661 atacttctgg acctgcaccg acgggcagac ggccgacggt ccgcagtttt cgcgaaccta
     3721 cggcaaaccg ggcgtctaca gcgaagtcct ggaagtgacc gatccccagg gccggcggca
     3781 ctacgatttt gcggtcgtca acgtgatcga ccgcgcccat cccgagcagt tgccgccgag
     3841 catccatgct tctttcgcgc cgaccgaagg tatcaaggcc ggcgaggcca tcaagtttct
     3901 cgtccgcacg tttcgcacca ccgacggcga agagacctgg gattttggcg acggctcgcc
     3961 gccggtcgta gtccacagcg acggcaacgc ggtccagctg gccaaggacg gctacgcgca
     4021 aaccgtgcac aagtatgcca agccgggaac gtatctcgtc cgcgtgcagc ggacgaacaa
     4081 gctcggcttc accgcgacag cgcggctgaa ggtaaccgtg ggtgagtagc gacgggctac
     4141 ttttttgcca gcacgcgatc gatcacgtcg gcatacgcca gcagggtgag gtagttgcgg
     4201 agcagataac cgttgcggga gaggctctcc tgcgagaagt cctggtgggc ctgcgggttg
     4261 gaaggatgca caccatcgcg gcagatgagc gtcggcactt cgtattcgct gccaggggtg
     4321 tccttgaatt tcgcgagcgc cccgtcccag tcgtccggac gccgcttcag gatttccgcc
     4381 tgatagtcga tcagcagaac ttgcttcgac ttcgcgacgc ggcggacggc gtcaccgaat
     4441 tgtttcgatt tctccagaat gccgctccgc ggcggcacgg tcgtcagcag caccaccgtc
     4501 ccgtttttca ggcagcgatc gacgacttcc gccgtcttct gctcgtattc ctggacgtcg
     4561 agctggctca ggtcattgga gccgaagagg atcacggcaa cttcaggatt gagcttcgtc
     4621 agccacgact cgacgttctc gcgggcccag cggatagtca ttccgccttc gttgccgttc
     4681 tccggtccgc gccactcgcg ccagcactgc ggctgttgat gccccctgac caggcgcagc
     4741 gccgcggcca tctccggcgg catctgcttg ggctcgccct ggagcggcgc ccagaaggcc
     4801 agcgagacgg tgatcgagtc gccaaacagg gccagcgtgc ccggcgtgcc cgtgaaccgc
     4861 gaccggacct ccttcatcgg ctcgaaccag gtcggggcgg attgggcccg cactgccgca
     4921 gccgtaagta ggacggcgcc caaggtggtc aagaccaacg acgacggtcg cactgccata
     4981 tcaccctcgc gtttctgctg cggccagctt acacagcccg ccgcgcagac tccacggcga
     5041 aaaaacttgc cccggcaaat cggctatcat gtcccggacg aatctccctc ttgcggtgag
     5101 cgccccatga acctccgcgc gttgttcttc gtggtcgtat cggcggcttg ctctgcggca
     5161 ctgccggcag cggagccgcc gcgctggaac atcctgttct gcttcgcgga cgactggggg
     5221 cggtatgcga gctgctacgc cgcggttgat gggcggccgt cgttgaacca ggtcgtaagg
     5281 acgccgaacg tcgatcgcgt ggcccgcgaa ggcgtgctgt tccgcaacgc ttttgtgaac
     5341 gcgccctcct gcacgccctg ccgcagctcg ctcctgtccg ggcgatattt tttcaactgc
     5401 ggccgcgggg cgatcttgca gggcgcgatt tgggattccg caattcccag ctttccgctg
     5461 ctcttgcgcg acgccggcta tcagatcggc aagagctaca aggtctggag cccgggcacg
     5521 ccggccgacg caccctttgg cgggcagcag tacgcctacg agcaggccgg ccgcctgccg
     5581 aacaatttct ccgaggaggc aacgaagctg atcgaggccg gcgcgaccgt agccgaggcc
     5641 cgcgaaaaaa tcctggctca ggttcgcgga aacttcgacg ccttcctgga caaacgcgag
     5701 cgtgggaaac cctggctcta ctggttcggc tcgacgacga cgcaccgcgt ctggatcaag
     5761 ggctcgggaa aaaagctgtg gggcatcgag cctcagtcgc tggccggcaa gctgcccaaa
     5821 ttcctgccgg acgtgccgga ggtccgcgag gacgtggccg attacctggg cgaatgccag
     5881 gcgttcgacg ccgaagttgg cgtattgctc aagcgattgg aggaggtcgg tgaattggac
     5941 cggacgctga tcgtcttgag cggcgatcac ggcatgccgg gcgtgccggc gggaaagtgc
     6001 aatctctacg atcacggcgt cagcgtcgcc ctggcgatcc gcgtgcctgg cggcaagggc
     6061 gggcgcatcg tggacgactt cgtgcggctg cccgacctcg cgcccacgtt tatggaagtc
     6121 ggcggcgcta ctcccccggc gggtctctat ggcaagtcgc tggtgccgct attgacgagc
     6181 gaaaagagcg ggcaaatcga ctccgaacgg aattgggtcg taactggccg cgagcggcat
     6241 gtgggtaatg cccgcgaaga gaacctgccg tatccgatgc gggcgctgcg gacgccgcag
     6301 ttcgtctata tccgtaactt cgccgcggac cgctggccga tgggctcgcc caaggaggcc
     6361 gcgagcgtgc cgcccccggc cggcgaggcc tgggagaaca acacgttcgc cggtttcgcc
     6421 gacatggacg gcagcccgac aaaggcctgg ctgatcgccc accagaaaga cactcact
//
LOCUS       DASRJB010000726         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4159733, whole genome shotgun sequence.
ACCESSION   DASRJB010000726 DASRJB010000000
VERSION     DASRJB010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159733"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFB80_19105"
     CDS             complement(<1..402)
                     /locus_tag="VFB80_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35950.1"
                     /translation="MPAEAVVATLRQTWDILGELHVPAALMGGLALTHWGYVRATQDV
                     DLLIALSGVRPTALLAKLAAAGYRAKRRDPLIRLDDAEFIQLFYVPKDEVIEVQIDLL
                     LAEAPFHRQAVARRVTLPHSALGFQLDVVSCE"
     gene            complement(392..637)
                     /locus_tag="VFB80_19110"
     CDS             complement(392..637)
                     /locus_tag="VFB80_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35951.1"
                     /translation="MRPDSATTTAGSSADRIARWLDLMRTSDKLLLAGLRRKIGPAGD
                     LTAAYRQWYADHMREHDAVVERIARLGRDCEESSDAR"
     gene            complement(692..1990)
                     /gene="aroA"
                     /locus_tag="VFB80_19115"
     CDS             complement(692..1990)
                     /gene="aroA"
                     /locus_tag="VFB80_19115"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZN35952.1"
                     /translation="MQDRLTIQPAGRPVRGRIRPPGSKSITNRALVCAALAQGTSTLG
                     GALDSEDTRVMIESLGRLGIAVESRDEGRTLIVHGCGGQIPVASAHLFVGNSGTTIRF
                     LTAMCALGHGMYRLDGVPRMRERPIGDLLDALRQLGVDVRCEGREGFPPVAVIANGLH
                     TGDLEATVRGEISSQFLSGLLMAIAAAGEYVRITVAGELVSKPYAAMTLKVIESFGGV
                     IEQDGWQEFLSRDCDPGYRGCNYAIEPDASAASYFWAAAAITGGEVTVEGLSKDSLQG
                     DVAFASCLAQMGCRVTCAADSITVGGRAERGIDVDMNAISDTVQTLSAVALFADGPTT
                     IRGVGHIRHKETDRIGDLARELRKFGAQVDESADGLRITPTRSASEGVPIDIETYHDH
                     RMAMSLALPGLVRPGVTILNPACTAKTYPKFFEDLAALTQ"
     gene            complement(2147..>3182)
                     /locus_tag="VFB80_19120"
     CDS             complement(2147..>3182)
                     /locus_tag="VFB80_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN35953.1"
                     /translation="PGVQISELMPKLAQRMNKLANIRSLDTKIGDHGQGARLMHLGRR
                     DEPSLKYPDLGAMLARELGRAESQVPDYVSFYTATEGRGSAISQSGFLGARYNAMFLT
                     EQNTPPNLRRLDELTELDHQERADLRDLLSARFARGRESAPLASHNEAYSRVRGLMAS
                     EKLFDISGEDQKVRDLYGPTLFGEQALIARRLVEAGVPFVKVSRAWWDSHGQNFETHL
                     ELVTELDRVYSALLDDLGSRGLLEDTLIVTLSEFGRTPQINASLGRDHFASAWSVSLA
                     GAGVRGGTVYGSSDEDGHNVKDNKVGAAEVFATIFRAVGIDHKKDYHVGSRPVPLTDP
                     GTKPIQDVLA"
BASE COUNT          517 a         1025 c         1094 g          546 t
ORIGIN      
        1 ctcgcaactg accacgtcaa gttggaatcc cagcgctgag tggggcagcg tgacgcgccg
       61 cgcaacagcc tggcggtgga agggagcttc ggccaacaac aggtcgatct ggacttcaat
      121 cacttcgtct ttgggcacat agaacagttg aatgaactcg gcatcatcca ggcggatcaa
      181 gggatctcgc cgtttggcgc gatagccggc agcggcgagc ttcgccagca gcgcggtggg
      241 gcgaactccg gagagcgcaa tcaacagatc cacgtcttga gtggcccgaa cgtagcccca
      301 gtgagtgagg gcgagtccgc ccatcaaggc agcgggaacg tgcagctcgc ccaggatgtc
      361 ccaagtctgc cgcagcgtcg ctaccacggc ctcagcgggc atcgctggac tcctcgcagt
      421 cgcggcccag ccgcgcgatg cgctctacca ctgcatcgtg ctcgcgcatg tggtcggcat
      481 accactggcg ataagccgcc gtcaggtcgc cagcggggcc aattttgcgc cgcaatcccg
      541 ccaagagcag cttgtcgctg gtccgcatca ggtccagcca gcgggcgatg cggtccgccg
      601 aagatcctgc tgtcgttgtg gcgctgtctg gtcgcatgga gttggtccac tggagccaat
      661 tatacgtcac cgaccgcgtg cagaggcatc gtcactgagt cagcgccgcc aggtcctcga
      721 agaacttggg atacgtcttc gccgtgcagg cggggttgag gatggtgacg ccggggcgga
      781 cgaggccggg gagggcgagg ctcatggcca tgcggtggtc gtggtaggtt tcgatgtcga
      841 tggggactcc ctcgctggcg ctgcgggttg gtgtgatgcg gaggccgtcc gcggactcgt
      901 ccacttgcgc gccgaacttg cggagctcgc gggccaggtc gccgatgcgg tcggtctctt
      961 tgtggcggat gtggccgacg ccgcggatgg tggtcggccc gtcggcgaac agcgcgacgg
     1021 ctgagagcgt ttgcaccgtg tcgctgatcg cgttcatatc gacgtcgatg ccccgctcgg
     1081 cgcggccgcc gaccgtgatc gagtcggcgg cgcaggtcac gcggcagccc atttgagcca
     1141 ggcaactggc gaacgccacg tcgccttgca gcgagtcttt cgagagcccc tcgaccgtca
     1201 cttcgccgcc agtgatcgcc gcggcggccc agaagtagct cgcggcggag gcgtcgggct
     1261 cgatggcgta gttgcagccg cggtagcccg ggtcgcagtc ccgcgacagg aactcctgcc
     1321 agccgtcttg ctcaatcacg ccaccgaacg attcgatgac cttcagcgtc atcgctgcat
     1381 agggtttgga gacgagttcg ccggcgacgg taatccgcac gtactcgccc gcggcagcga
     1441 tcgccatcaa gaggccgctc aagaactggc tggagatctc gcctcggacg gtcgcctcca
     1501 agtcgcccgt atgcagtccg ttcgcaatca cagccaccgg tgggaacccc tcgcggcctt
     1561 cacatcgaac atccactccc aattgccgga gcgcatcgag caggtcgcca atcggccgct
     1621 cgcgcatccg cggcacgccg tccagccggt acatgccgtg ccccagggcg cacattgcgg
     1681 tcagaaagcg gatcgttgtg ccgctgttgc cgacaaacag gtgcgccgag gcgaccggaa
     1741 tctgcccgcc gcagccgtga acaatgagcg tccgtccttc gtcccgcgat tcaaccgcaa
     1801 tacccagccg gccgaggctc tcgatcatca ctcgcgtgtc ttcgctgtcg agcgcgccgc
     1861 cgagcgtgct cgtgccctga gccagcgcgg cgcagacgag agcgcggttg gtgatgctct
     1921 tggagcccgg cgggcggatg cggccgcgga ccgggcggcc ggcgggctga atcgtgaggc
     1981 ggtcttgcac cttagagatc gtacacgcgt tcgatctcgt gcccaagctc cgcttgggca
     2041 cgcaagtctc gaagctccgc ttcgacggca ggagaccgga agcagagctt ccagacgtgg
     2101 gttcccaagc ggagcttggg aaccagaacc ggcgtggacg ccggggctaa gcgagcacgt
     2161 cctgaatcgg cttggtgccg gggtcggtca gcgggaccgg gcggctgccg acgtggtagt
     2221 cctttttgtg gtcgatgccg acggcgcgga agatcgtggc aaacacctcg gccgcgccga
     2281 ccttgttgtc cttcacgtta tgtccgtcct cgtcgctgct gccatagacc gtgccgccgc
     2341 ggacgcccgc gccggccagc gaaacgctcc aggccgaagc gaagtggtcg cgtcccaggc
     2401 tggcgttgat ctgcggcgtg cgaccgaatt ccgagagcgt gacgatcagc gtgtcctcca
     2461 acagcccgcg gctgcccagg tcgtcgagca gcgccgaata gacgcggtcg agctcggtga
     2521 ccagctccag gtgcgtctcg aagttttgtc catggctgtc ccaccaggcc cgcgagacct
     2581 tgacgaacgg cacgcccgcc tcgaccaggc ggcgggcgat gagggcctgc tcgccgaaaa
     2641 gcgtcggacc atacaggtcg cggactttct ggtcttcgcc ggagatgtcg aacagctttt
     2701 cgctggccat caagccgcgg acgcgggagt aggcctcgtt gtggctggcc agcggggccg
     2761 attcgcggcc gcgggcaaag cgggccgaga gcaggtcgcg cagatcggcc cgctcctggt
     2821 ggtcgagctc ggtcagctcg tccagccgtc ggaggttggg cggcgtgttc tgctcggtga
     2881 ggaacatcgc gttgtagcgt gcgccgagga agcccgactg gctgatggcg ctgccgcggc
     2941 cctcggtggc ggtgtagaag ctgacatagt cgggcacctg gctctcggcc cggcccagct
     3001 cgcgggccag catcgcgccg agatccggat acttgagcga cggctcatcg cggcggccca
     3061 ggtgcatcag ccgtgcgccc tggccgtggt cgccgatctt ggtgtcgagc gaacggatgt
     3121 tcgcaagctt gttcatccgc tgggcgagct tgggcatcag ctcggaaatc tgaacgccgg
     3181 ga
//
LOCUS       DASRJB010000727         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3035329, whole genome shotgun sequence.
ACCESSION   DASRJB010000727 DASRJB010000000
VERSION     DASRJB010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035329"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFB80_19125"
     CDS             complement(<1..1220)
                     /locus_tag="VFB80_19125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35954.1"
                     /translation="MSEEQIFLAALDQPDAAARAKHLDEACQGNAQLREQVEKLLAAH
                     FQSGEFLDVPAPQQVQIETSAGDAVTILTMGLSAHNEAAAMNADDDSGDLEFLAPTTR
                     PDSLGRIGHYEVLQVIGRGGFGTVLRAFDDVLQRVVAVKVMAPQLAATSPARKRFLRE
                     ARTSAQVRHENVVQVYEVGEQPLPYLVMEFIPGETLQQRLDRIGPLEVPEVVRIGRQI
                     AEGLAAAHATDLIHRDIKPGNVLLEGGHLKVKITDFGLARAGDDASMTQSGMIAGTPM
                     YMAPEQVLGQMLDQRADLFSLGTVMYQMASGRVPFRAPNSVAVLKRVADDTPRDIREV
                     IPETPQWLCDIIAKLHAKNPDHRYQSARDVADVLADCEEQLKQNSRLRDYSRIPRNKS
                     EPAAGPFKWGVAAAL"
     gene            complement(1302..1898)
                     /locus_tag="VFB80_19130"
     CDS             complement(1302..1898)
                     /locus_tag="VFB80_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35955.1"
                     /translation="MPQLQHKLGGMSDVTRILSQLDAGDPTAAEQLLPLVYHELRRVA
                     AAKLAQEKPGHTLQATALVHEAYLRLVDPVDRKAWDHRGHFFAAAAEAMRRILVDGAR
                     KKAATKRGGDLGRVDLEPDLAVAPEVREDLVALDAALDRLAAEYPVQAELVKLRYFGG
                     LSLPDAAAALGLAERTAGRHWAFARAWLRRAVEGSQES"
     gene            complement(1970..3202)
                     /locus_tag="VFB80_19135"
     CDS             complement(1970..3202)
                     /locus_tag="VFB80_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN35956.1"
                     /translation="MTQKIHTHPLPSGLTLVAEEMPWLESAAFALLVPAGCCYEPADK
                     LGLASLTCEMAQRGAGERDSRRFLADLENLGAETSASVSIAHTSVGGSMPAESLPQVL
                     GLFADLVQRPHLPEDQLEDARLGCLQEIRAVEDDLAQKTMQQLRLRHYAAPWGRSSQG
                     AEPTVSGLSMADVRRHFQTHYSPKNAILSVAGKIDWPRLLDDVQRLFGEWQAAAAAGV
                     QETPADRSYLHIPTESSQTHIGIATDAVPYSNADYFQIRGAVGVLSDGMSSRLFTEVR
                     EKRGLVYTVYAMCHSLKDRGAILSYAGTTAERAQETLDVMLAELTGLYRGITSDEIDR
                     LKGRIKRALIIQQESSPSRAGSIALDWYYLSRIRTMAELSGIIDGLTAESINRYLAAN
                     APRAFSVVTIGQQQLVSQ"
     gene            complement(3311..3874)
                     /locus_tag="VFB80_19140"
     CDS             complement(3311..3874)
                     /locus_tag="VFB80_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN35957.1"
                     /translation="MGIVYFKRFRMEVDLARPLFPQPGLPEEYRLAAWDDGLLEAHAE
                     TKFRCFRWELDANVFPSLGERDGCRRLMTEIARQRGFIPQATWLLEYWPDNARRPELV
                     GTIQGVSDDKVGAIQNVGITEPHRGRGLGTVLMWHSLAGFQAAGIERVYLEVTAQNRG
                     ACRLYERLGFQQTKVVYKASEVAYSEW"
BASE COUNT          719 a         1462 c         1404 g          809 t
ORIGIN      
        1 agcgcggcgg ccacgcccca cttgaaagga cccgcagcgg gttctgattt gtttcgcgga
       61 atccggctgt agtcccgcag ccgcgaattc tgcttgagct gctcttcgca gtcggccagt
      121 acgtcggcca cgtcccgggc cgactggtag cggtggtcgg ggttcttagc gtgcagcttg
      181 gcgatgatgt cgcacagcca ctgcggcgtt tccgggatga cttcgcggat gtcgcgcggc
      241 gtgtcgtcgg cgacccgctt gagcaccgcg accgaattcg gggcccgaaa tggcacgcgg
      301 ccggaagcca tctgatacat cacggtgccg agactgaaca gatcggcccg ttggtcgagc
      361 atctgcccga ggacttgctc gggcgccatg tacatcggcg tgccggcgat catgccgctt
      421 tgggtcatgc tggcgtcgtc gcccgcgcga gccaggccga aatcggtgat cttgaccttc
      481 agatgtccgc cttcgaggag cacgttgccc ggcttgatgt cgcggtgaat caagtccgtc
      541 gcatgggccg ccgccagccc ctcggcaatc tggcgcccga tccgcacgac ttcgggcacc
      601 tcgagcggac cgatccggtc cagccgctgc tgcagcgtct cgcccggaat gaactccatc
      661 accaggtagg gcagcggctg ctcgccgacc tcatagacct gtacgacatt ctcatgccgc
      721 acctgagccg aggtgcgggc ctcgcgcagg aaccgcttgc gggccggcga ggtcgccgcg
      781 agttgcgggg ccatcacctt gaccgccacg actcgctgca gcacgtcgtc gaacgcgcgg
      841 agcacggtgc caaatccgcc ccggccgatc acctgcaaga cctcgtaatg cccgatccgg
      901 ccgagcgagt cgggccgcgt cgtcggcgca aggaattcca agtcgcccga atcatcgtct
      961 gcgttcatgg cagcagcctc gttatgggca gacaggccca tcgtcagaat cgtgaccgca
     1021 tcgccggcgc tggtttcgat ctgaacctgc tggggcgccg gcacgtcgag aaactcgccg
     1081 ctctggaaat gcgcggccaa caacttttcg acctgctcac gaagctgcgc attaccctgg
     1141 caggcttcgt ccaaatgctt ggcccgagcg gctgcatcgg gttggtcgag cgcggccaga
     1201 aagatttgtt cttcgctcac ggcagcggtt cctccgcgga ttgcgcgata gctgtctcca
     1261 gccccaatgc gtattttcgc tgcagagctg gccaagattt ttcaggattc ttggctacct
     1321 tccaccgccc gccgcagcca ggcccgggca aacgcccagt gccggcctgc cgttcgctca
     1381 gccaggccca gggcagccgc tgcatcgggc aggctcagcc ccccgaaata gcgcagcttg
     1441 accagttccg cctgcaccgg atattccgcg gccagccggt cgagcgccgc atcgagcgcg
     1501 acgaggtctt cccggacctc cggggcaacg gccaggtccg gctccaagtc cacccgacca
     1561 aggtcgccgc cacgtttggt ggccgccttc ttccgcgccc cgtccactag aatccgccgc
     1621 atcgcctccg cggctgccgc gaagaagtgc ccgcgatggt cccacgcttt ccggtccacc
     1681 ggatcgacca gccgcagata tgcctcgtgc accagtgcgg tggcctgtag cgtgtgcccg
     1741 ggcttctcct gggccagctt ggccgctgcc accctgcgca gctcgtgata gaccaagggc
     1801 aatagctgct cggcggccgt cggatcgccg gcatcaagct gtgacaggat gcgggtcaca
     1861 tcgctcatgc cgcccagctt atgttgcaac tggggcattc tccagtgcag agcactccta
     1921 tcgacctcgg ctctgccggc atcactacgt cccgcacgcc cggcgggacc tactgggaca
     1981 cgagctgctg ctggccgatg gtgacgaccg aaaacgcccg cggggcgttg gcggcgaggt
     2041 agcggttgat gctctcggcg gtgagcccgt cgatgatgcc gcttagctcg gccatggtgc
     2101 ggatgcggct gaggtaatac cagtcgaggg cgatcgagcc ggcccgggag gggctgctct
     2161 cctgctgaat gatgagcgcc cgcttgatgc ggcccttgag gcggtcgatc tcgtcggagg
     2221 tgatgccccg gtacagcccc gtcagctcgg ccagcatcac gtcgagcgtc tcctgggccc
     2281 gctcggcggt cgtgccggcg taggacagga tcgccccgcg gtccttcagg ctgtggcaca
     2341 tcgcgtagac cgtgtaaacc agcccccgct tctcgcggac ctcggtgaac agccgcgagc
     2401 tcatgccgtc gctgagcacg ccgaccgcgc cgcgaatctg aaaatagtcg gcgttcgaat
     2461 agggcaccgc gtccgtggcg atgccgatgt gcgtctggct cgactcggtg ggaatgtgca
     2521 gatagctgcg gtcggcgggc gtttcctgga cgccggccgc cgctgcggcc tgccactcgc
     2581 cgaacagccg ctgcacgtcg tccaggagcc gcggccagtc gatcttgccg gcgacgctga
     2641 ggatcgcgtt tttgggcgaa tagtgggtct gaaaatgccg ccgcacgtcg gccatcgaca
     2701 ggccgctcac ggtcggctcg gcgccctgcg agctgcgccc ccagggcgca gcatagtgcc
     2761 gcagccgcag ctgctgcatc gtcttctggg ccaggtcgtc ttcgaccgcg cggatttcct
     2821 gcaggcagcc gaggcgggcg tcttcgagct ggtcctcggg caggtgcggc cgctgcacga
     2881 gatcggcaaa caggcccagc acctgcggga ggctctcggc gggcatcgag cccccgaccg
     2941 acgtgtgggc gatcgagacc gacgccgagg tttccgcacc caggttctcc aggtccgcga
     3001 ggaaccgccg gctatcgcgc tcgcccgcgc cgcgctgggc catttcgcag gtcaggctgg
     3061 ccaaccccag cttgtcggcc ggctcatagc agcagcccgc cggcaccagc agggcgaacg
     3121 ccgccgattc gagccagggc atttcttcgg cgaccagcgt caggccggaa gggagcgggt
     3181 gcgtgtggat tttctgagtc acagaagaca attcagcgga gggcgaggca caaatgacaa
     3241 atgacaaatg accagtgaca aagcagtcat ttgtcacttg ccatttgtca tttgtatcgc
     3301 tttcttaccc ctaccactcc gagtacgcaa cctcagacgc cttgtagacg accttggtct
     3361 gctgaaagcc gagccgctcg tagaggcggc aggccccgcg gttctgggcg gtgacttcca
     3421 ggtagacccg ctcgatgccg gcggcctgga aaccggccag cgagtgccac atcagcaccg
     3481 tgcccaggcc gcggccgcgg tggggttcag tgatgccgac gttttggatc gcgccgactt
     3541 tgtcgtccga gaccccctgg atcgtgccca ccagctccgg ccggcgggca ttgtccggcc
     3601 agtattcgag cagccacgtg gcctggggga tgaagccgcg ctggcgggcg atttcggtca
     3661 tcagccggcg gcagccgtcg cgctcgccga gcgaggggaa gacgttggca tcgagttccc
     3721 agcgaaagca gcggaatttc gtctcggcgt gggcctccag gagcccatcg tcccacgccg
     3781 ccaggcgata ttcctccggc agccccggct gcggaaacag cggccgcgcc agatcgacct
     3841 ccatgcgaaa ccgcttgaaa tagacgatgc ccacgacgct gccttctcgg tccagacgat
     3901 gtgttttcgg cgacgccgac tcctaaggat accactcgct aatgctgcaa tcagcttttt
     3961 gggaggggca aacttgcgtt tcctgagaac cgccaagcgg ccgggcgtcg aatgcgagcc
     4021 acggagcagc ctgcctcatg ccgatcggct gcagttcgga ataaacccgt ttgctttccc
     4081 agaagtggga ttaggatcgg cctgcggatt catttgggat ccaatttagg gtccggctct
     4141 cgcaattatg accttccgct acaaactccg cacactcttg attgccttga cgctggggcc
     4201 gcttgtcggt gcgattggct ggatttcgtt cccagtctgg ctgccatttg tctggcaatg
     4261 cattgggatc ggcatattgg cgcaagccct agcttggtcc attaggcgcg ctggtttcta
     4321 cgattaagag tcacccatgt tccgtttcac gatccgcgag ctagtcctgg tgacagcggt
     4381 cgtggcgctc acca
//
LOCUS       DASRJB010000728        14910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1336653, whole genome shotgun sequence.
ACCESSION   DASRJB010000728 DASRJB010000000
VERSION     DASRJB010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336653"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2984)
                     /locus_tag="VFB80_19145"
     CDS             complement(<1..2984)
                     /locus_tag="VFB80_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35958.1"
                     /translation="MTAIDRAGRAARLLARCPVAAALALVAALASPLRAGVELPVGNQ
                     TGAISIRAGGATHWKQGAYEVWVLGGDVQITQGDVVAKSREAVLWIDRAEAFSGRPSK
                     IIAYLEKFVQVDFGRGGDPHAITGRRAQSLVDEQSWIGRFHTTGGIELAAPLTSHEPQ
                     VRPAIFARGNSARENEFKPAPQAAAAELPYAVAPSQAVQPAQFTREEIAPPAGTPVAP
                     AGNRVVIEGRGSRGFSPHYFTDAERNERIVVSTTGVIVRIDGIDQLGSVRIDCDRVVT
                     WVPNVAPGSPAFNQAIQSGEGPLEFYLEGNIVFRQGDRVIYADRMYYNVIQQQGVVLN
                     AEMLTPVPQYRGYLRLKADVLRQVNRQRFEAYNAGLTSSRMGIPRYWFQSQNVAVDDV
                     QTPIVDPFTGLPLLAPDGEPQVDHQLLATARNNTIHLAGVPIFYWPTIATDLTEPTYY
                     LTGFRFRNDSVFGTGAQLDWDLYQLLGIEDKPPNTKWTLSTDYFNLRGPALGTNFGYQ
                     GNTLFGVSGPYRGFFDAWGIHDDGTDNLGREWRDLAIPNDIRGRVLARHRQYLPNDFI
                     LSAELGLISDYNFLEQYYELEWDTFKDQSTGVELKKLLGNSSLAISGDVRPDGFVTDT
                     EWLPRADHYLFGQPLLFDRLTWHAHSSVGYAHMREASPPVDPAQVAVISPLAWEADVQ
                     GLRAATRHELDLPLELGPAKVVPYILGEAAYWGEDLNGQSLTRGYAQAGVKASVPIWR
                     ADPTVGSELFNLTGLAHKITLEAEYFFADASQDLADLPLYDPLDDNATEFTRRQMAVR
                     TFGQAQGTFVPLQFDERFFALRSNLQGSVSSPVEIADDLTALRLGINQRWQTKRGLPG
                     QQRIIDWIVFDLQAVLFPDEAQNFGSAIGLVDYEFRWHVGDRLTLLSDGYYDGYDQGL
                     RQATVGALISRPEYGNVYLGFRSTEGPISSNIATGSLSYRMSEKWIATAGAMWDFGST
                     GNIGQNFALTRIGESFLIRLGF"
     gene            3281..3832
                     /locus_tag="VFB80_19150"
     CDS             3281..3832
                     /locus_tag="VFB80_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35959.1"
                     /translation="MNRLLALSVALWASMTIAAAAQEQPQQQPAVPPVEATKHVVVTW
                     TLGPQEWDFSDVMTTYVPVSGVLDTNKPPAAEGAPADNSAGRAIWTVEIARNLLEGEV
                     KVHTEAEGSPFKAVLLDADKVALGADLRIVFTKITGKKGDKLKVICVLPPEEVFKLTK
                     FIRIERRTKVGFETPTVIGGDRR"
     gene            complement(3829..3999)
                     /locus_tag="VFB80_19155"
     CDS             complement(3829..3999)
                     /locus_tag="VFB80_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35960.1"
                     /translation="MALMLLFLLISAGNFCLGFALAVHFGHGPQGIDLATPVRLLHAL
                     RARVRGKARAGR"
     gene            complement(4123..9990)
                     /locus_tag="VFB80_19160"
     CDS             complement(4123..9990)
                     /locus_tag="VFB80_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN35961.1"
                     /translation="MNKTRRSILSRTLQGLRRAWRRRQRRSEQGRSFRLEALEPRLAL
                     TISAPLPSATDHIHPVLKIMIEGEQVTIGPNIGLTQTTHFNPHTHDFTGTLHIGEGGP
                     AGIGSTVRNVTLQDFFDVWRTSNVGQVTNNPNAIFDTDPDDGTSAPRILNKTVDAGHV
                     LRMYVKEPGDAVAELEYDSSSATNSLERPELYVPRDGDQVTISYDAIATAADSPSFAP
                     IANQTLLVGAPLWLALDGFDPNGGPLTYSVQVGNTSLLQASVPTGNRSVAIDVASVGT
                     MKFQLFDNLVPDVTNRIAQLINAGFYNKTASNQITFHRVINNFMIQAGDPTGTGSGGS
                     TLPDFDDKFHLDLQHTTSGLLSMAKSGDDTNDSQFFITDVPTRHLDFNHSIFGRLTEG
                     DVLRDTINSVPVDDDDKPLSPVVINSISFINDTENGVLMLKAAPGATGSTSVTVTVTD
                     AQNHSFSRTFQVNLTPDTSNSAPFLQPIAEPVRGVVGQSIQLQLAAVDAEGNPNFFDA
                     VRPTSETVNYTVTPNNNTGLVVITPPAGFVGKFNVLVGVSGATQTNTSDPFDTQLVEV
                     DVAPAAPTVDLLAASDSGVSNSDNITNAGTLQFQISGVTNGALVKLLKGTTVLAQGTA
                     SGTSIALSTTDIAAAGEGVHGITATQTVSGIESNASALLNVTYDITPPPAFTSTPPTE
                     GDVGIALQYNAQNPEESSAGFRYELVTQPAGATIDNTTGVLNWTPLAAQIGPQTFGIL
                     ASDAAGNVRTQSVSVVVSQPLPPKVDLVLELTQPNGSPLTSLNSGQSFILKVSTRDLR
                     TPSRGVFSAYMDILWDSTKATVNGNITYGSEYGTQPSGTASPGLIDEVGAVAGNNELG
                     GGLHHLFSIPMVAGAAGSLIFVADPADSLPAHDALVYGENAAVNDKVRFGSVSISINA
                     SFNAVNDTFNVNEDAVNATLNPLLNDTNIGNNQNVLTISAVGSTSSGGTVTIAPDGKS
                     LKYSPAANFSGAETFTYTARNQNNETNVATITVQVQPSNDPPSGVNDSFNVPEDSQNF
                     VLDVLGNDLTSPDTGETLRVTQVSAGSAGGTLTIGNNGANIRYTPALNFLGTETFTYT
                     LSDRTTGGLTSTASVTLTVGEVNDPPVATSDSATVAEDSNETTINVVANDGIGPDTGE
                     TISVTAVASASAGGTISVADGGQAVIYKPAADFQGTETFTYTLTDSRGGTATGVVTVT
                     VSNSNDPPTAVNDNLTGFKDTTSTFDVLANDSSAPDPTENLVIDSITQPQHGTLTIID
                     GGKKVSYTPTAGYTGPDSFTYVVRDPGGLLTEPATANITVQEFAPSTLSGFVYFDVDN
                     DGQHDAAELPLSGVTITLTGTTINSQSVNVTVKTLHDGSYAFENLAPGNYTLKQTQPS
                     FTIDGQDTAGSQGGTVTNDQIVIANLAQGTEGENNNFGERGRALSTISIADLFSSNSR
                     DYAIAALDSTGQELWHSTSGPDWGDYTNTAFSLPSTAQLRVQATNSLAQVVAATLNTT
                     SDMLGLIGENSGSKLFRVRGGPDNLSFIPFANQAPVGVGDSYNATEETVLTVPSAGVL
                     SNDTDAEGNPLTASIVTLPTKGSLSFNANGSFIYTPNVNATGADSFTYRASDGTNQSA
                     PVTATISIAGVNDAPLAAADAYTANAGTPLVVAAADGVIDNDTDVDQQTLTATVLTQP
                     SGGAVTLAANGGFTFTATAGFSGLTTFTYRVTDGTLTSDATVSLTVNRPPTAANDDYA
                     VDEDEALTVAVADGVLDNDSDPDGNPLTAIVVSQPQHGTLTLAANGSFVYTPDDDFEG
                     TDGFSYKANDGHADSTVAAVTITVNPVNDAPVAAANSFTTPQNTQLTIDAPGVLAGDT
                     DVDEDTLTATVITDPDHGTLDLESDGSFTYTPDEGYFGPDSFVYRASDGTLHSQATVS
                     ITVEEPGEGGQGGEGESAGELALLSWLSDHEDDGHSHDHGGDWQSAVDEAFGELG"
     gene            10429..10971
                     /locus_tag="VFB80_19165"
     CDS             10429..10971
                     /locus_tag="VFB80_19165"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZN35962.1"
                     /translation="MSRIAVAFRAFFAALASGQRAEQIRAVLDGEALPKIDTAAKTAA
                     PPARLESPVPSRSEAITLLAALQREARLVDLIQQPLGEFSDEQIGAAARNVLTDSAAV
                     LDRFFALRPIAAGEEGAACDVPKNHDPARYKLAGSVSGPGPFQGRLSHPGWQATTVKL
                     PSWTGAKDSALVIAPAEVDV"
     gene            complement(10986..12050)
                     /locus_tag="VFB80_19170"
     CDS             complement(10986..12050)
                     /locus_tag="VFB80_19170"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35963.1"
                     /translation="MRTSILAAILGWLALAEARGQNIELVAGGGETPEGVAATKAKLG
                     NPFGVDFDAAGNLFFVEIDGHRVCRIDPAGKLTRIAGTGVKGDAGDGGPALSAQFNAL
                     HNLAVGDGGDIYLADTLNHKVRKLDKSGQITTIAGTSKGFSGDGGPAARAQFSGIYCV
                     SLDPSRERLYLADLDNRRIRMIVLKTGIVTTVAGNGEKGVPADSAAAAESPLVDPRAV
                     AADSAGNVYVLERSGHALRVVDRQGKIRTLAGTGQRGATGDGGPARQATLSGPKHLCI
                     DKDGGIIIADTDNHVIRKLLADGTIVRLAGSGRRGPGGVPGPALAVELDQPHGVYVHP
                     SGDLYIADSLNHRILKIDRP"
     gene            12169..14802
                     /locus_tag="VFB80_19175"
     CDS             12169..14802
                     /locus_tag="VFB80_19175"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN35964.1"
                     /translation="MRIRTALACYLAVVCSGRLQSAELVPTAWYTPSELLTLLSEREQ
                     LRWACPETLAGRALVGEAANTHALLDDACRQWGLAWTKSNGIVVVHRADDERLKTLTA
                     ALARGDRAAAWELGWLRDGRAIPPLAAALVSSDVAVALAAAQAIETLDSLAPLGRDER
                     VDAFPAGRVSLAVAFPPNADLQPLLASPYPPLRAAALRLLFAKRGDAAALAKYHTAGD
                     PSAAVRRVRQQLLGPMPSNKPDEHIITALLPLPAPAEGEAACAKLVGELPALEKRSEW
                     EQMRWRVRCLAAWSRAGRQPATKALSELCSTKLQFGWFPGYAQMHLAATGSPEATARV
                     REILPKADRDTLVRGLEQSHYGERLLALTNSYLDEPTVCYVTARKAGREALDDLRAWA
                     GRGNGAAIDALGVVGGEQASASLRAQLEKETPGSGTLAFRSAKALGRIGSSESLAALL
                     AAAASPDRFRRHAGALFLGQIGGPQAISRLRELLEKETDRLVRAAAADALEQIGDKVS
                     AEAVAAFRQADASVPPRIYRPRNPRFGDEFPVNQWVNLKIGIQAEAAFGEMGWNYDAA
                     NRLFFRYGGCSGYTNELTLLDLGTEQFVQRRPNEEMAGWGDRRASRGCSGGRTWDPFL
                     KVAWIGPVIGGTESDLAITEYYNKGGEYRLCSYDLATDRFRAAAYLDRTYGEPSKRYA
                     YDWQRGLLIPVKFSPFKEEKPFWVIDTKSSDPYAASAWLDRKTTGDYPRDAGYQTAAF
                     DQRSGLLVLYVAPRDGKPAETWTYDPASNTWQNKQPPVQPAGVHGAGFVYEPFQKVLL
                     LQSGKRETQYGGGSDSITWTYDAGANTWTDLNVASGPGNPWVGAMDFDPEHNVFVLFN
                     HRDRQVWAYRYKQVAVGTRAE"
BASE COUNT         2346 a         4767 c         5170 g         2627 t
ORIGIN      
        1 ttgaagccca ggcgaatcag gaacgattcg ccgatccgcg tgagggcgaa gttctggccg
       61 atgttgcccg tcgagccgaa atcccacatc gcgccggccg tggcgatcca tttctcgctc
      121 atgcggtagc tcagcgagcc ggtggcgatg ttcgagctga tcgggccttc ggtcgagcgg
      181 aagccgagat agacgttgcc gtattccggc cggctgatca gcgccccgac ggttgcctgg
      241 cggaggccct ggtcgtagcc gtcgtagtag ccgtcggaga gcagcgtcag ccggtcgccg
      301 acgtgccagc ggaattcgta atcgaccagg ccgatcgccg agccgaagtt ttgtgcctcg
      361 tcggggaaga gcacggcttg caaatcgaat acgatccagt cgatgatccg ctgctggcct
      421 ggcaggccgc gtttggtctg ccaccgctgg ttgatgccca gccgcagggc ggtgaggtcg
      481 tcggcgatct ccaccggcga gctgaccgag ccttggaggt tgctccgcag ggcgaagaac
      541 cgctcgtcga actggagcgg aacgaaggtg ccctgggcct ggccgaacgt gcggacggcc
      601 atctgccggc gggtgaactc ggtggcgttg tcgtcgagcg ggtcgtacag cggcaggtcc
      661 gccaggtcct ggctggcgtc ggcaaagaag tactcggcct ccagcgtgat cttgtgggcc
      721 aggcccgtca gattgaaaag ctcgctgccg acggtcggat cggcccgcca gatgggcacg
      781 ctggccttca cgccagcctg ggcatagccg cgggtgagac tctggccgtt cagatcctcg
      841 ccccaatacg ctgcttcccc gaggatgtag ggcacgacct tcgctggtcc cagctccagc
      901 ggcaaatcga gctcgtgccg cgtcgccgcg cggaggcctt gcacgtcggc ctcccaggcg
      961 agcgggctga tcaccgcaac ctgcgccgga tcgaccggcg gcgacgcttc gcgcatgtgg
     1021 gcgtagccga cggacgagtg cgcgtgccac gtgaggcggt caaagagcag cggctgcccg
     1081 aagaggtaat gatcggcccg cggcagccac tcggtatccg tgacgaagcc atcgggccgc
     1141 acgtcgccgc tgatcgccag gctgctattg cccagcagtt tcttcagctc gacgccggtg
     1201 ctctggtcct tgaaggtgtc ccactccagc tcgtaatact gctcgaggaa attgtagtcg
     1261 ctgatcaggc ccagctcggc cgagaggatg aaatcgttcg gcagatactg ccggtgtcgg
     1321 gccagcacgc ggccgcggat atcgttgggg atcgccaggt cgcgccattc gcggccgagg
     1381 ttgtcggtgc cgtcgtcgtg gatgccccag gcgtcgaaga agccccggta gggaccgctc
     1441 acgccaaaca gcgtgttccc ctggtagccg aagttcgtgc ccagggccgg gccgcggagg
     1501 ttaaagtagt cggtcgagag cgtccacttc gtattcggcg gcttgtcttc gatccccagc
     1561 agctgataca ggtcccagtc gagctgcgcg cccgtgccga agacgctgtc gttcctgaag
     1621 cggaagccag tcaggtaata tgtcggctcc gtgaggtccg tggcgatcgt cggccagtag
     1681 aagatcggca cgccggccag gtggatcgta ttgttccgcg cggtcgccag cagctggtga
     1741 tcgacctgcg gctcgccatc cggcgcgagc agcggcagcc ccgtgaacgg atcgacgatc
     1801 ggcgtctgca cgtcgtccac cgccacgttc tgcgactgga accagtaccg cggaatgccc
     1861 atgcggctgc tggtcaggcc ggcgttgtag gcctcgaacc gctggcgatt gacctgccgc
     1921 aacacgtcgg ccttcagccg caggtagccg cggtattgcg gcaccggcgt gagcatttcg
     1981 gcgttcagca cgacgccctg ctgctggatg acgttgtagt acatccgatc ggcgtagatg
     2041 acgcggtcgc cctggcggaa gacgatgttg ccttccaggt agaactccag cggcccctcg
     2101 cccgactgga tggcctggtt gaaggccggg ctgcctgggg cgacgttggg cacccaggtc
     2161 acgacgcggt cgcagtcgat tcgcaccgag ccgagctggt cgatgccgtc gatgcggacg
     2221 atcacgcccg tggtggagac gacgatccgc tcgttccgct ccgcatccgt gaaatagtgc
     2281 ggcgagaacc cgcgactgcc gcggccctcg atgaccacgc ggttaccggc cggcgcgacc
     2341 ggcgtgccgg ccggcggagc gatttcctcg cgggtgaact gggcgggctg gacggcttgc
     2401 gagggcgcaa cggcgtaggg gagttcagcc gcagcagcct gcggcgcggg cttgaattcg
     2461 ttctcgcggg ccgagttgcc gcgggcgaat atcgccggcc gcacttgcgg ctcgtggctg
     2521 gtaaggggcg cggccaactc gatgccgccg gtggtgtgga agcggccgat ccagctctgc
     2581 tcgtccacga gcgactgcgc gcggcggccg gtgatcgcgt gcggatcgcc gccacggccg
     2641 aaatcgacct gaacgaactt ctccaggtag gcgatgatct tgctcggccg gccgctgaat
     2701 gcctcggccc ggtcgatcca gagcacggcc tcgcggcttt tggcgaccac gtcgccctgc
     2761 gtgatctgca cgtcgccgcc caggacccag acttcgtacg cgccctgttt ccagtgcgtc
     2821 gcgccccccg cgcggatcga gatcgcgccg gtctggttgc cgaccggcag ctcgaccccc
     2881 gcgcgcagcg gcgaggccag cgccgcgacg agcgcgagcg cagcggcgac cgggcagcgc
     2941 gcgagcaggc gcgcagcgcg accagcgcga tcgatggcgg tcaaaatgcg agtcagttcg
     3001 gcttccttgc caggcgtcgt tcgctgttgc gcagagcagg cgcgcacctg ccccgccaga
     3061 agcgggccca aaaaggcggg cgaagtatag aaatgccgcc gggggctcac aatcgcaagg
     3121 tgcgacggcg acgacagtta gctaagccgc gaccactcgt cgcggcttgg gggcgaagca
     3181 acattgacag cacgccgcgg ttgaaggtag tgtgcgcgcg ctcgctggcc ccatgcctgc
     3241 gggcccgtcc ggcagtgctg ctagctacaa ggaattgctc atgaatcggc tgttggcact
     3301 cagcgtggcg ctttgggcaa gcatgacgat cgccgcggcg gcgcaagagc agccgcagca
     3361 gcagccggcg gtgccgccgg tggaagcgac gaagcacgtg gtggtgacgt ggacgctggg
     3421 cccccaggag tgggacttca gtgacgtgat gaccacctat gtgccggtga gcggcgtgct
     3481 tgacacgaac aagccgccgg ccgccgaggg cgctccggcg gacaacagcg ccggcagggc
     3541 aatctggacg gtcgagatcg cgcggaacct gctcgaaggc gaggtcaagg tgcacaccga
     3601 ggcggagggt tcgcccttca aggcggtgct gctggacgcc gacaaggtcg cgctgggggc
     3661 ggacctgaga atcgtcttta ccaagatcac gggaaagaag ggcgacaagc tcaaggtgat
     3721 ctgtgtgctg ccgccggagg aagtcttcaa gctgacgaag ttcatccgca tcgagcgccg
     3781 cacgaaggtc ggcttcgaga cgccgacggt gatcggcggc gaccggcgct agcggccggc
     3841 tcgcgctttg ccgcgaacgc gggcccgcaa ggcatgcagc agtcggaccg gcgtcgccag
     3901 gtcgattccc tgcggaccgt ggccgaagtg gacggccagc gcaaagccga ggcagaaatt
     3961 cccggcggaa atcagcagaa acagcagcat gagcgccatc gcgacctcgc agagttagtg
     4021 cgcaagtcta ggtcgcaaat agaactcaga atgcagaatt cagagttccg tatttatcac
     4081 tatcccctga actctgacct ctaacctcta acctctgaat ccctacccca gctcgccgaa
     4141 cgcctcatca acggccgatt gccagtcgcc gccgtggtcg tgcgagtggc cgtcgtcttc
     4201 gtgatccgac agccaggaca agagcgccag ctcgcctgcc gactcgcctt cgccgccctg
     4261 gccgccttcg cccggttctt cgaccgtaat cgatacggtg gcctggctgt gcagcgtgcc
     4321 gtcggaggcc cgatagacga agctgtctgg gccaaaatag ccttcgtccg gcgtgtaggt
     4381 gaagctgccg tcggactcca ggtcgagcgt gccgtggtcc ggatccgtaa tgacggtcgc
     4441 cgtgagcgtg tcttcgtcga cgtcggtgtc gccggccagc acgcccggcg cgtcgatcgt
     4501 cagctgcgtg ttttgcggcg tcgtaaagct gttcgccgca gccactgggg cgtcgttgac
     4561 gggattcacg gtgatcgtca ccgcagcgac cgtgctgtct gcgtgaccgt cattggcctt
     4621 gtagctgaag ccgtcggtcc cctcgaaatc gtcgtcgggc gtgtagacga acgagccgtt
     4681 ggcagccagc gtcagcgtgc cgtgctgcgg ctgtgagacg acgatcgcgg tcagcgggtt
     4741 gccgtcgggg tcggaatcgt tgtcgagcac gccgtcggcg actgcgaccg tcagagcttc
     4801 gtcttcgtcc accgcatagt cgtcgttggc ggcggtcggg ggccggttga cggtgaggct
     4861 caccgtggcg tcgctggtca gcgtgccgtc ggtgacgcga taggtaaacg tcgtcaggcc
     4921 gctgaagccg gcagtggctg tgaacgtgaa tccgccattg gcggccagag tcaccgcgcc
     4981 gccagacggc tgggtgagga ccgtggcagt cagggtctgc tgatcgacgt cggtgtcgtt
     5041 atcgatcacg ccgtcggcgg ctgccacgac gagcggcgtg ccggcgttgg cggtgtaggc
     5101 gtcggccgcg gccagcggag catcgttgac gccggcgatc gagatcgtgg ccgtcaccgg
     5161 agcactttgg ttcgtgccgt cgctcgcgcg atacgtgaag gagtccgcgc cggtggcgtt
     5221 gacgttcggc gtgtagatga acgaaccatt ggcattgaac gacagcgagc ccttcgtcgg
     5281 cagcgtgacg atcgaagccg tcagcgggtt gccctcggcg tcggtgtcgt tgctcaagac
     5341 gccggcggaa ggaacggtga gcacggtctc ttccgtggcg ttgtacgagt cgccgacgcc
     5401 gaccggggcc tggttggcga agggaatgaa gctcagattg tccggtccgc cgcggacgcg
     5461 gaacagcttc gagccgctgt tctcgccgat caggccgagc atgtcgctgg tggtgttgag
     5521 cgtggccgca acgacttgcg ccaaggaatt ggtcgcctgc acgcgcaact gcgccgtgga
     5581 aggcagcgag aaggcggtgt tggtgtagtc gccccaatcg gggccgctcg tcgagtgcca
     5641 cagctcctgg cccgtgctgt ccagggccgc gatagcgtag tcccgcgagt tcgacgagaa
     5701 caggtcggcg atcgagatcg tgctgagtgc ccgcccgcgc tcgccgaagt tgttgttctc
     5761 gccctccgtg ccttgcgcca ggttggcaat cacgatctga tcgttcgtca cggtgccgcc
     5821 ttgcgagccg gcggtgtcct ggccatcgat ggtgaagctg ggctgcgtct gcttgagcgt
     5881 gtagttgccc ggggcgaggt tctcgaaggc gtacgagccg tcgtgcagcg tcttgaccgt
     5941 cacgttgacg ctctgactgt tgatcgtcgt gcccgtgagc gtgatcgtga cgcccgacag
     6001 cggcagctcc gcggcgtcgt gttggccgtc gttatcgacg tcgaagtaga cgaagccgct
     6061 cagcgtgctg ggagcgaact cctgcaccgt gatattggcc gtcgccggtt ccgtcaggag
     6121 gccgcccgga tcccggacga cgtacgtgaa gctgtcgggg ccggtgtagc ccgccgtggg
     6181 cgtgtagctg actttcttgc cgccgtcgat gatcgtcagc gtgccgtgct gcggctgcgt
     6241 gatcgaatcg atgaccaggt tctcggtcgg atcgggggcc gagctgtcgt tggccagcac
     6301 gtcgaacgtc gaggtcgtgt ccttgaatcc ggtcaggttg tcgttaacgg ccgtcggcgg
     6361 atcgttcgaa ttgctgaccg tgaccgtgac cacgccggtg gccgtgccgc cgcggctgtc
     6421 ggtcagcgta taggtgaacg tttcggtgcc ctggaagtcg gccgccggct tgtagatcac
     6481 agcctggccg ccgtcggcca ccgagatcgt accgccggcc gaagcgctag ccaccgcggt
     6541 gacggaaatg gtctcgccgg tgtcggggcc gatgccgtcg ttggccacca cgttgatcgt
     6601 cgtctcgttg ctgtcttcgg caacggtggc ggagtcgctc gtggccaccg gcggatcgtt
     6661 gacctcgccg acggtcaggg tcacgctggc ggtcgaggtg agaccgcccg tggtccggtc
     6721 gctgagcgta taggtgaagg tctcggtgcc caggaaattc agggccggag tgtagcggat
     6781 gttggcgccg ttgttgccga tcgtaagcgt gccgccggcg ctgcccgcgc tgacctgcgt
     6841 cacccgcagc gtctcgccgg tgtcgggcga ggtcaggtcg ttgcccagca cgtcgagcac
     6901 gaagttctgg ctgtcttccg gcacgttgaa cgagtcgttg acgccgctgg gcgggtcgtt
     6961 cgacggctgc acctgcacgg tgatcgtggc gacgttggtt tcgttgttct gattccgggc
     7021 ggtgtaggtg aaggtctctg caccgctgaa gttagcggcc ggcgaatact tcaggctctt
     7081 gccgtccggg gcaatcgtca ccgtgccgcc gctggacgtc gatccgacgg ccgagatcgt
     7141 cagcacgttc tggttgttgc cgatgttcgt gtcgttgagc agcgggttga gcgtggcatt
     7201 caccgcgtcc tcgttcacgt tgaacgtgtc gttgacggcg ttgaacgagg cattgatgct
     7261 gatcgagacg ctgccgaagc ggactttgtc gttgactgct gcattttcgc catagaccag
     7321 ggcgtcgtgc gccggcaggc tgtcggccgg atcggcgacg aagatcagac tgcccgccgc
     7381 gcccgccacc atcggaatgc tgaacaggtg gtgcagaccg ccgcccagct cgttgttgcc
     7441 ggcgaccgcg ccgacttcgt cgatcaggcc cggcgaggcg gtgccgctcg gctgggtgcc
     7501 gtattcggag ccgtaggtga tgttcccgtt gaccgtggcc ttggtgctgt cccagaggat
     7561 gtccatgtag gccgagaaca cgccgcgcga cggcgtccgc aggtcgcggg tggagacctt
     7621 gaggatgaag ctctggcccg agttgaggct cgtcagcggc gagccgttgg gctgcgtcag
     7681 ctccagcacc aggtccacct tcggcggcag cggctggctg acgacgacgc tcaccgactg
     7741 cgtgcggacg ttgcccgcgg catcgctcgc caagatcccg aacgtctgcg ggccgatctg
     7801 cgcagccagg ggtgtccagt tgagcacgcc ggtggtgttg tcgatcgtcg cgccggcggg
     7861 ctgagtgacc agttcgtagc ggaagccggc cgagctttcc tccgggttct gggcgttgta
     7921 ctgcagggcg atgcccacgt cgccttcggt cggcggcgtg ctggtaaagg cgggcggcgg
     7981 cgtgatgtcg taggtcacat tgagcagcgc cgaggcgttg ctctcgatgc cgctgacggt
     8041 ctgcgtggcg gtgatgccgt ggactccttc gcccgccgcc gcgatgtccg ttgtgctgag
     8101 cgcgatcgat gtgccgctgg ccgttccctg ggccaacacg gtcgtgccct tgagcagctt
     8161 gaccagcgcg ccattcgtga ctccgctgat ctggaattgc agcgtgccgg cgttggtgat
     8221 gttgtcgctg ttgctgacgc cgctgtcgct ggcggcgagc agatcgacgg tgggcgcggc
     8281 cggggcgaca tcgacctcga cgagctgcgt gtcgaaggga tcggacgtgt tcgtctgcgt
     8341 cgcacccgag acgccgacca gcacgttgaa cttgccgacg aagcccgccg gtggcgtgat
     8401 gaccaccagg cccgtgttgt tgttgggcgt gacggtgtag ttgaccgtct cgctcgtcgg
     8461 ccgcaccgcg tcgaagaaat tcggattgcc ctcggcgtcc accgccgcca gctggagctg
     8521 gatcgactgg ccgaccacgc cccgcaccgg ctcggcgatc ggctgcagga acggcgcact
     8581 gttgctggtg tcgggcgtga ggttgacctg gaacgtccgc gagaagctgt ggttctgcgc
     8641 gtcggtcacc gtgaccgtga ccgacgtgct gccggtggcg cccggggccg ccttcagcat
     8701 cagcacgccg ttttccgtat cgttgatgaa gctgatcgag ttgatcacga ccggcgacag
     8761 cggcttgtcg tcgtcgtcca ccggcacgct gttgatcgtg tcgcgaagga cgtcgccttc
     8821 ggtcaggcgg ccgaagatcg agtggttgaa gtccaggtgc cgcgtgggca cgtcggtgat
     8881 gaagaactgc gagtcgttgg tgtcgtcacc cgacttggcc atcgacagca ggcctgaagt
     8941 ggtgtgctgc aaatccagat ggaacttgtc gtcgaagtcg ggcagcgtcg aaccgccgct
     9001 gccggtgccg gtcggatcgc cggcctggat catgaagttg ttgatcaccc ggtggaacgt
     9061 gatctggttc gaggcggtct tgttatagaa gccggcgttg atcagctggg cgatgcggtt
     9121 ggtgacgtcc gggacgaggt tgtcgaacag ctggaacttc atcgtcccca cgctggcgac
     9181 gtcgatcgcg acgctgcgat ttcccgtggg aaccgatgcc tgcaacaggc tcgtgttgcc
     9241 cacctgcacc gaataggtca gcgggccgcc gttgggatcg aagccgtcca gcgccagcca
     9301 caagggcgca ccgacgagca gcgtctggtt ggcgatcgga gcaaacgacg ggctgtcggc
     9361 cgcggtggcg atcgcgtcgt agctgatcgt gacctgatcg ccgtcgcgcg gcacgtacag
     9421 ttccggccgc tcgaggctgt tcgtcgccga actgctgtca tactccagct ctgcgaccgc
     9481 atcgcccggc tccttcacgt acatccgcag cacgtgcccc gcatccaccg tcttgttcag
     9541 aatccgcggc gccgatgtgc cgtcatcggg atcggtgtcg aaaatcgcgt tgggattatt
     9601 cgttacttgc ccgacgttcg acgtccgcca gacgtcgaag aagtcctgga gcgtgacgtt
     9661 ccgcaccgtc gaaccgatgc cggccgggcc gccctcgccg atgtgcagcg tgccggtgaa
     9721 gtcgtgcgtg tgcgggttga agtgcgtcgt ctgcgtcagg ccgatgttgg gcccgatcgt
     9781 cacctgctcc ccctcgatca tgatcttcag gaccggatgg atatggtccg tcgctgacgg
     9841 cagcggcgcg gagatcgtga gcgccagccg cggctccaag gcctccaggc ggaagctccg
     9901 cccctgctcc gagcggcgct gccgccgccg ccaagcgcgg cgcagtccct gaagcgtgcg
     9961 gctgaggatg gaacggcgag tcttgttcat gcttctctgt tcaaggtgac gaggacccgg
    10021 gcaagcgcta cctaacggtt cgatcccagc cgcgccctcg tggcgcatgg aatggggcaa
    10081 caagccgacg gctgatgtgg gttccggacc actggcagcc gggcgggggg aagatcatct
    10141 tcccccagag tgagcaggcg cgcgatgggc ttgcgccggc agtgtttcag cataaccgga
    10201 agtgctgcag ccgtcatggg tttggcagga atcggcaggc caattgacaa tgccgccggc
    10261 cagccgctgt actggcaggc ggaatggatg tgcgccattg gtgtcgcaaa agccggtccc
    10321 gagcttcgca gcccttagac gttgcgggcg gcaaaatcgt tccgcgccct gccagctgac
    10381 ctccgggccc gccaatcaac ttgacataac cccttgcagg atcagcagat gagtcgaatt
    10441 gccgtggcct tccgcgcgtt ctttgccgcc ctggccagcg ggcagcgggc ggagcagatt
    10501 cgggcggttc tggatggcga agcgttgcca aaaatcgaca cggcagcaaa aacggcagca
    10561 ccgccggctc gcctggaatc ccccgtgccc tcgcgcagcg aggcaatcac gctgctggcc
    10621 gccctccaac gggaagcccg cctggtcgat ctcatccagc agccgctggg cgaattctcc
    10681 gatgaacaaa tcggggcggc ggcccgcaac gtcctgaccg actcggcagc cgtgctcgac
    10741 cggttcttcg cgctgcggcc gatcgccgcc ggggaagaag gggccgcctg cgacgttccc
    10801 aagaaccacg accccgcccg ttacaagctc gccggctccg tctccggccc cggccccttc
    10861 caaggccgcc tctcccaccc cggctggcaa gccaccacgg tgaaacttcc cagctggacc
    10921 ggtgccaaag actccgccct ggtcatcgcc ccggcggagg tggatgtctg acgaactgcg
    10981 gcatgctagg gccggtcgat tttcaggatg cggtggttga ggctgtcggc gatgtagagg
    11041 tcgccgctgg ggtggacgta gacgccgtgc ggctgatcga gctcgaccgc cagggcgggg
    11101 ccggggactc cgccggggcc gcggcggccg ctgccggcga ggcggacgat cgtgccgtcg
    11161 gcgaggagct tgcgaatcac gtggttgtcg gtgtcggcga tgatgatgcc accgtccttg
    11221 tcgatgcaca gatgcttggg gccgctcagc gtcgcctggc gggccgggcc gccgtcgccg
    11281 gttgcgccac gctggccggt gccggcgagc gtgcgaattt tgccctgccg atcgacgacc
    11341 cgcagcgcgt gccccgaccg ctccaggaca tagacgttgc cggcgctatc tgcggcgacc
    11401 gcccgcggat cgaccagcgg cgactcggcg gcggctgcgc tatctgcagg tacgcctttt
    11461 tcgccgttac cggccacagt ggtaacgatt cccgtcttga gcacgatcat gcggatgcgg
    11521 cggttgtcga ggtcggccag gtacagccgc tcgcgggatg gatcgaggct gacgcagtaa
    11581 atgccgctga actgggccct cgccgcgggg ccgccatcgc cgctgaatcc cttgctcgtg
    11641 ccggcgatcg ttgtgatctg cccgctcttg tcgagcttgc ggaccttgtg attgagcgtg
    11701 tcggccaggt aaatatcgcc gccgtcgcca acggccaggt tatgcaaggc gttgaactgc
    11761 gcggacaggg ccgggccgcc gtcgcctgcg tcgcccttga cgcccgtccc ggcaattcgc
    11821 gtcagcttgc ccgccggatc gatgcggcag acgcgatggc cgtcgatctc cacaaagaac
    11881 aagttgcctg ccgcgtcgaa atcgacgccg aacggattgc cgagcttcgc cttggtcgcg
    11941 gccacgccct ccggcgtctc tccgccgccg gcgacaagct cgatgttctg ccctcgcgcc
    12001 tcggccaagg ccagccagcc caatatcgcc gccagaatcg atgtgcgcat cgctcgtctc
    12061 cctcaccaga attccaatgc cgtggaccgc atcataacaa ctagcgcttt gccctgccgc
    12121 gcttggccgc attatcctgg tagccgcaaa acccgccgga gatcggtcat gcgaattcgc
    12181 accgcgttag cgtgctatct ggcagtcgtc tgcagcgggc ggttgcaaag cgccgagctg
    12241 gtcccgacgg cctggtacac gcccagcgag ttgctgactc tgctgagcga gcgagaacaa
    12301 cttcgctggg cctgccccga aacgctggcc gggcgcgcgc tcgtcggcga ggcggcgaat
    12361 acgcacgccc tgctcgacga cgcttgccgg cagtggggcc tggcttggac caagagcaac
    12421 ggcatcgtcg tggtacaccg cgccgacgac gagcgattga agacgctgac cgcagcgcta
    12481 gcccgcggcg accgcgcggc tgcctgggag ctgggctggc ttcgcgatgg ccgcgccatt
    12541 cccccgctgg cggcggcgct cgtcagctcc gatgttgccg tggccctcgc cgccgcccag
    12601 gccatcgaga cgctcgattc gctcgcgccg ctggggcgcg acgagcgcgt cgatgcgttt
    12661 ccggcaggcc gcgtcagcct cgccgtcgcc tttccaccga acgccgactt gcagccgctg
    12721 ctcgcttcgc cctatccgcc gctgcgggcg gcggccctgc gactgctgtt cgccaagcgc
    12781 ggcgacgcag ccgcgctggc caagtaccac accgcgggcg accctagcgc cgccgtgcgg
    12841 cgcgtgcggc agcagctcct cggtccgatg ccctcgaaca aaccggacga gcacatcatt
    12901 accgccctcc tgccgctgcc agcgcccgcc gagggcgaag cggcgtgcgc caagctggtc
    12961 ggcgagctgc cggcgctgga aaaacgtagc gagtgggagc agatgcggtg gcgcgtccgc
    13021 tgcctggccg cctggtcgcg cgcgggccgg cagccggcga cgaaggccct cagcgagctc
    13081 tgttccacaa agctccagtt cggctggttc ccgggctacg cccagatgca cctggccgcg
    13141 actggcagcc ccgaggccac cgcgcgagtc cgcgagatcc tgcccaaggc cgatcgcgac
    13201 acgctggtgc gcggcctgga gcagagccac tatggcgagc ggctgctggc cctcacgaat
    13261 tcatacctgg acgagccgac agtctgctac gtgacggccc gcaaagccgg gcgcgaggcg
    13321 ctcgacgatt tgcgggcctg ggccggccgc ggcaatggcg cggcaatcga cgcgctggga
    13381 gtcgtcggcg gcgagcaggc ctcggcgtcg ctgcgggcgc agttggagaa ggagacgccc
    13441 ggttccggaa cgctggcctt tcgcagcgca aaagccctgg ggcgcatcgg cagcagcgaa
    13501 tcgctcgccg cattgctcgc cgcagcggcc agccccgatc gattccggcg gcacgccggg
    13561 gcgctgttcc tgggccagat cggcgggccg caggcgatta gccgtttgcg ggaactgctc
    13621 gaaaaggaga ctgaccggct ggttcgcgcc gcagctgccg atgccctcga gcagatcggc
    13681 gataaagtga gcgcggaagc ggtcgcagcc ttccgccagg ccgacgccag cgtgccaccg
    13741 cgcatttacc gcccacggaa tccgcggttc ggcgacgagt ttccggtcaa ccaatgggtg
    13801 aacctgaaaa ttggcatcca ggccgaagcc gccttcggcg agatgggctg gaactacgac
    13861 gctgccaacc ggctcttctt ccgctacggc ggctgcagcg gctacaccaa cgagctgacc
    13921 ctgctggacc tcggcacgga acagttcgtg cagcggcggc cgaacgaaga gatggccggc
    13981 tggggcgacc ggcgggcctc tcgcggctgc tcgggcggcc gcacctggga tccgtttttg
    14041 aaggtcgcct ggatcgggcc ggtgatcggc ggcactgaaa gcgatctggc gatcaccgag
    14101 tattataaca aggggggcga gtaccggctg tgcagctacg acctggcgac cgaccgcttt
    14161 cgcgccgcgg cgtatctcga ccggacgtac ggcgagccgt cgaagcggta tgcctacgac
    14221 tggcagcgcg gcctgctaat tcccgtcaag ttttcgccgt tcaaggagga gaagccgttc
    14281 tgggtcatcg acacgaagtc gagcgatccg tacgccgcgt cggcctggct cgaccgaaag
    14341 accacgggag actatccgcg cgacgccggc tatcaaacgg cggcgttcga tcagcgcagc
    14401 ggcctgctgg tgctttacgt cgcgccgcgg gacgggaagc ccgccgagac ttggacctac
    14461 gatccggcca gtaatacgtg gcagaacaag cagccgcccg tgcagccggc gggagttcac
    14521 ggcgcggggt ttgtctatga gccgtttcag aaagtgttgc tgctccaaag cggcaaacgc
    14581 gaaacgcaat acgggggcgg cagcgattcc atcacctgga cctacgacgc cggcgcgaac
    14641 acttggactg acttgaacgt ggcgagcggc cccggcaacc cctgggtcgg cgcgatggat
    14701 ttcgatcccg agcacaacgt cttcgtgctg ttcaaccacc gcgaccgaca ggtgtgggcc
    14761 tatcgctaca agcaggtcgc cgtgggcacg cgtgctgaat gaggagcaat tccagatgaa
    14821 agctcgaaca cccgtcgcag cgctggctgc atgcctggcg gcgctcgtac tctcgccact
    14881 gcgagcggcc gactggccac agttcatgcg
//
LOCUS       DASRJB010000729        10450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_212351, whole genome shotgun sequence.
ACCESSION   DASRJB010000729 DASRJB010000000
VERSION     DASRJB010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212351"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1208)
                     /locus_tag="VFB80_19180"
     CDS             complement(456..1208)
                     /locus_tag="VFB80_19180"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN35965.1"
                     /translation="MSQKLAGKVAVVTGASKGIGAQIARQLAADGAAVVVNYSSSKEG
                     ADKVVAEITKEGGRAVAVKANLSKPAEVEGLFIDTKKKFDRLDILVNNAGIYEFAPLE
                     SVTPEHFHKQFDLNVLGLVLATQQAAKHFGAAGGSVVNVSSVVATLAPPNTAVYSATK
                     AAVNAITRSLAQELGPRRIRVNAVNPGMVETEGTRSAGITDSDFRKTVEAQTPLGRIG
                     QPQDIAPAVAFLASDDAAWITGETLYISGGLR"
     gene            1560..4697
                     /locus_tag="VFB80_19185"
     CDS             1560..4697
                     /locus_tag="VFB80_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN35966.1"
                     /translation="MWIVQLALKRPYTFAVMALLIVVLGVAAISQMRTDIFPEIDIPV
                     VTVIWTYKGMDASEVEKRITTYSEYAFSSNVNGIKRMESQTLSGVAVIKIYFHPGTDI
                     GTALSQVTSASQAIRALMPPGIQPPIIVRFNASSVPILQIGLSSDTMSEEEVYDYGLY
                     ILRNQLSTVQGLTLPTPYGGRERQVMVDLDPRLLQAKSISPKEVADAISAYNLAAPTG
                     VARIDTHEYPVTLNNTPPTPEQFNDIPIKVVGGATVYMRDVAQVRDGSTSQTTIVRRN
                     GSRGALVTLLKNGNASTLDIVNRVKQMMPQIRAAAPSDLRIDLLFDQSLFVTAAIGGV
                     VTESIIAGLLTATMILVFLGSWRSTLIVAVSIPLAILSSIGALFLLGHSLNVMTLGGL
                     ALAVGILVDDATVEIENIHRNLGLGKPLRQAILDGAMQIAGPTFVSTLTISIVFVSVL
                     FLDGAPQYLFTPLALAVAFAMLASYLLSRTIVPTMVDYLLPAELNRGHGSGWFGRLHA
                     AFERRFEQFRDAYVALLQWNLQHRLAVFAIFGLIVASGLLILPRVGRDFFPTVDTGQF
                     RLHVRAPAGTRVEETERYFSQVEAAIREILPPHAVELVLDNIGLPNRTYAMAFGDSAT
                     TSMADGEILVALAHHRSHSTPEYMAQLRRELPERFPNLTFFFQPADIVSQILNFGLPA
                     PIDVQIAGLNRPQNIALASEIAERIRQIPGATDVHLHQVMNVPKLHIDVDQTRARELG
                     LTQQDVSNSVLVSLSGSGQVQPNYWVDPRMGISYLVETRTPTYKLDSVDSINALPLSS
                     AAGGPQLLANVATVERSVTPEVANHTNVQPTFDVYANVQGRDLGSVAREINRVLAEFR
                     TKLGPGDTIAMRGQVESMNSAFVRLGLGIAFAAVLVYLLMVVNFQSWLDPFIIITALP
                     GAFVGIVWSLFLWQTTFSVPALMGAIMSIGVATANSILLVTFANEQRGEGLPALEAAL
                     ESGRTRLRPVLMTAAAMIIGMLPMSLGLGEGGEQNAPLGRAVIGGLLIATLTTLLFVP
                     VVYSLLRRGSAPAAE"
     gene            4844..6280
                     /locus_tag="VFB80_19190"
     CDS             4844..6280
                     /locus_tag="VFB80_19190"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN35967.1"
                     /translation="MSVASDQGLIESKVSLGRSTMRVPALVERQARRELARENAPRRG
                     LTKAAWAAGFAVLGGMALAAGVEAWRSEQAASAAVESHVEPAKTVTILRPTPAAHSSV
                     TLPATFRPWQTATLHARASGYLTAWHRDLGAVVKAGELLAEIETPELDQEVAEGEALA
                     SEAAAAAVQARAERTEAEAELKVVEAQLARVQAEYELGKSQLSRRDKLVQSRSISQEE
                     LDTYQRQLQARAAEVAAAQSDVARRRTNLQTRAAIIAAREATAKSRQSNVERLKELQA
                     FKRIVAPFDGVITGRIAEVGMLVTAGKEPLFVIEDLSKIRVQLSVPQTYAMQTAPGVA
                     ATIRLPESQTQPVMAAITRIAESVDAASRTMLAEIELDNVEHHFQPGSYAQVTLAAPQ
                     SSSSWSIPTNALLMKTNGPHVALVNDQNQIEVKRVTLGRDLGDRVIVAEGIHGGERLV
                     VNPGDGVATGVQVVVHQPADAARKFAER"
     gene            6415..7020
                     /locus_tag="VFB80_19195"
     CDS             6415..7020
                     /locus_tag="VFB80_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN35968.1"
                     /translation="MAVDESPLTRASLLVQIRDGANHSAWHEFVQLYGPVVYGFARKR
                     GLQDADAADLMQDVLRSVSSAIGRLDYDRRQGTFRGWLFTITRNKILNFFAARKIRPQ
                     GSGDTTTNRMLDAQPSDAGDDAWELEYQRRLAALAMDRIKGEFQDKTWQAFWLMAVEG
                     QSAAEVGRRVGLSPGAIYVAKSRVLARLKDEVEALRQQEEV"
     gene            7022..9229
                     /locus_tag="VFB80_19200"
     CDS             7022..9229
                     /locus_tag="VFB80_19200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN35969.1"
                     /translation="MKTPCPATEELRELLGGLAPGPQQEVWTQHLDQCECCQAKLEEL
                     AIGGTNLSQVVQQLHQAVPAAQSAYWPALRAMEQAPALAQTLAPETPSPEVKQRLRDS
                     STNFLLPPTDPAYLGRLERFDVMRVIGRGGMGIVLEAFDSRLQRNVALKVLDPELASD
                     ETARQRFCREARAAASITHENVVAVHQVEKAADNGLPYLVMQLVAGETLEQRLLREKR
                     LPLKEIVRIGLQAAQGLAAAHAQGLTHRDVKPGNILLEPPHDKVKLTDFGLARIADDV
                     KLTRTGFVTGTPLYMAPEQALGESADPRSDLFSLGAILYEMCAGQPPFQGNSALAILK
                     QIAESKHRPLRELNPDVPEWLAEMVDELLAKKPEDRYQTAADLAEVLDFHWAHMRSSS
                     ADLPAVCQTELKQRAARNRWIFAGVSVAVLAVGLLAGMVLPRLASWTRALASAAKPAA
                     VLPANAGAVWSVAFDPQSNTVAMAVEDGSVRLWDWPTKAVQSTFEAHRGVVWASRFSG
                     DGKYIATAGDDGLLKIWNRTSLESLQEFRHPNAVRGLAIGAGDKFFAGDRNGGLHVWS
                     LGQDKPLVTAEQPGAVYTVAISPDGKTLATAGSDKIVRLWHAETLKQRLPLEGHAGPI
                     YSLSFNPEGSRLASAGWDGTIRIWDPAGGKLLHSWEGHRGDIWAIAFSPSGTRLATGG
                     QDGSLKLWDAATGQLLATYLGHTSTIHTIAFNADGTLLASGGRDGAARVWRIR"
     gene            9602..10000
                     /locus_tag="VFB80_19205"
     CDS             9602..10000
                     /locus_tag="VFB80_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35970.1"
                     /translation="MAFSIFDDPPRVSWENKPVEMNADEALSLENDGASGETALDRAV
                     GWLRCRLEGGPLPANELKDQAELDGIAPRTLDRAKAVLGVIVKPSGFGGPRIWWLPGD
                     DRVRQATKRTPTTKQWRRLAILAHSVGRLS"
     gene            10081..>10450
                     /locus_tag="VFB80_19210"
     CDS             10081..>10450
                     /locus_tag="VFB80_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35971.1"
                     /translation="MSIGKLLRSISPADAEAFRVWLATEANDRDNGRDELSDNTVRRR
                     TGIAKQFFRAAQKRHLIESNPFSRLAVCEWLGNSQAVALEHYAPVTDDHFRAATGEPV
                     RRAEAKSEAEAKQFPKQQASA"
BASE COUNT         1897 a         3337 c         3367 g         1849 t
ORIGIN      
        1 cttccgtttg ccccgcacac gtgacttggt cagaagtgct gtacaacaac ttaaaacgat
       61 cgccgacgga gccgcataag cacggcagtt agaatcacat tatgaaacga gttagcccgc
      121 ggcaattcac catcgccaat actctctgtt tgacgttcac tgccgccctc gtgggagcgg
      181 cggaccgaga catacacgcg gtgcgaaagg atggcgtact cctggcaccc attctgccgt
      241 caaatcacac tggtcggcat gctccgccac cggctctagt tcggccctta tcgtacgcac
      301 ctcttccgca tcggccagcg cgtcggggat gagcgccgga ggctagtccg catcgtcggg
      361 ctgagcgatg gccggatacc gtggcccatc ggccagcagg ggcgaactag aaccatcgtg
      421 ctctacggcg ggctgcccgt gctgtacggc gcgtatcaac gcaggccgcc ggaaatgtag
      481 agcgtttcac cggtgatcca ggccgcgtcg tccgaggcga ggaacgccac ggcgggggcg
      541 atgtcttgcg gctggccgat gcggcccagc ggcgtttggg cttccaccgt cttgcggaag
      601 tcgctgtcgg tgataccggc cgagcgcgtg ccttcggtct cgaccatgcc ggggttgacc
      661 gcgttgacgc gaatcctacg cggccccaat tcctgagcca gcgagcgggt gattgcgttg
      721 actgcagctt tggtggcgct atagaccgcc gtgttgggcg gagcgagcgt cgccacaacg
      781 gagctgacgt tgacgacgct gccgccggcg gcgccaaaat gcttcgcggc ctgctgcgtc
      841 gccagcacga ggcccagcac gttcaggtcg aactgcttgt ggaaatgctc gggcgttacg
      901 ctttcgagcg gggcgaattc gtagatgccg gcgttgttga cgagaatgtc cagccggtcg
      961 aatttcttct tcgtgtcgat gaacagccct tcgacttcgg ccggtttcga caagttcgct
     1021 ttcaccgcga ccgccctgcc cccttcctta gtaatttcgg cgacgacctt atcggcccct
     1081 tccttgctgc tggaataatt gaccacaacg gctgcgccat cagccgccag ttgtcgggcg
     1141 atctgggctc cgatcccctt cgacgcgccg gtgacgacgg ccactttgcc cgcgagtttc
     1201 tgagacatga ctgtgctcct ttcaaacaaa tgggtgaaac ctcaaatacg ttaggttcgc
     1261 aagtcgggtg gcggtgttcg gtggaccgcg gcgtcgttgc gtaacaccag gctttaccgg
     1321 gcaggcggcg gactcttaca cgaagacgca aagatcttcc taagggctgg gaaattagag
     1381 agaaaaccgc ggatttcgta ggtgcccgga tggaaaggcc gtaagaatgc ctcttgccgc
     1441 atccgcctga aatccgcggt tcttccattc gccgtctgca gatttcgcgc cgtgtaagat
     1501 cgccgcgggc cggctgtatt tccttgcgca gcccacttca aactgccaag gaatcagcca
     1561 tgtggatagt ccagctcgcc ctgaagcggc cttatacgtt tgcggtgatg gccctgctca
     1621 tcgtcgtgct cggcgtcgcg gcgatcagcc agatgcggac cgatatcttt ccagaaatcg
     1681 acatcccggt cgtcacggtc atctggacct acaaagggat ggatgccagc gaggtcgaga
     1741 agcggatcac gacctacagc gagtacgcct tttcgtccaa cgtgaacggc atcaagcgga
     1801 tggagtcgca aacgctgagc ggcgtcgccg tcatcaaaat ctactttcat ccgggcaccg
     1861 acatcggcac ggccctgtcg caagtgacct ccgccagcca ggcgatccgg gccctcatgc
     1921 ccccgggcat tcagccgccg atcatagtcc ggttcaacgc ctcgagcgtg ccgattcttc
     1981 aaatcggcct ctccagtgac accatgtccg aggaagaggt ctatgactac ggcctctaca
     2041 tcctccgcaa ccagctctcg accgtgcagg gcctgacgct gcccacgccc tacggcggcc
     2101 gcgaacggca ggtgatggtc gatctcgacc cgcggctgtt gcaagccaaa agcatctcgc
     2161 ctaaggaggt ggccgacgcg atcagcgcgt acaacctggc cgccccgacc ggcgtggccc
     2221 gcatcgacac gcacgaatac ccggtcacgc tcaacaacac gccgcccacg cccgagcagt
     2281 tcaacgatat ccctatcaag gtcgtcggcg gcgcgaccgt ctatatgcgc gacgtggccc
     2341 aggtccgcga cggcagcacg tcgcaaacca cgatcgtccg ccgcaatggc agccgcgggg
     2401 cgctcgtcac gctgctgaaa aacggcaacg cgtcgacgct cgacatcgtc aaccgcgtca
     2461 agcagatgat gccgcagatc cgggccgccg cgccgtcgga tttgcggatc gacctgttgt
     2521 tcgatcaatc actgtttgtc acggcggcga ttggcggtgt ggtgaccgaa agcataatcg
     2581 ccggcctgct gacggcgacg atgatccttg tgtttctcgg cagttggcgg agcacactga
     2641 ttgtggccgt gtcgattccg ctggcgatcc tgtcgtcgat cggcgcgctg ttcctgctgg
     2701 gccattcgct gaacgtaatg acgcttggcg gcctggccct ggcggtcggc attctggtcg
     2761 atgacgcgac ggtcgagatc gaaaacatcc accgcaacct cggccttggc aagccgctgc
     2821 ggcaagcgat tctcgacggg gcgatgcaga tcgccggccc cacgttcgtc tcgacgctga
     2881 cgatcagtat cgtgtttgtc tcggtgctgt tcctcgatgg cgctccccaa tacctgttca
     2941 cgccgctggc gctggccgtg gcgttcgcga tgctcgcctc gtacttgctc tcgcggacga
     3001 tcgtgccgac gatggtcgat tacctgctgc ccgccgagct gaaccgcggt cacgggtccg
     3061 gctggttcgg ccgcttgcac gcggcgttcg agcggcggtt cgagcagttt cgcgatgcgt
     3121 acgtcgcgct attgcagtgg aacttgcaac atcggctcgc cgtgtttgca atctttggtc
     3181 tgatcgtcgc cagcgggcta ctgatcctgc cgcgggtcgg ccgcgacttc tttcccaccg
     3241 tcgatacggg ccagttccgg ctgcacgtca gggccccggc cggcacacgc gtggaagaga
     3301 ccgagcgtta tttcagtcag gtcgaagccg cgatccggga gatcctgccg ccacacgcgg
     3361 tcgagctggt gctcgataac atcggcctgc ccaatcgcac ttatgcgatg gctttcggcg
     3421 acagtgccac gaccagcatg gccgacggcg aaatcctcgt cgctctggcg catcaccgct
     3481 cgcattcgac gcccgagtac atggcgcagt tgcggcgcga gttgcccgag cggtttccga
     3541 acctgacgtt cttctttcag ccggccgaca tcgtcagcca gattttgaat ttcggcttgc
     3601 cggcgccgat cgacgtccag atcgctgggc tgaatcgtcc gcagaacatc gccctggcca
     3661 gcgaaatcgc cgagcggatt cgccagattc cgggagcgac cgacgtgcac ctgcaccagg
     3721 tgatgaacgt gccgaagctg cacatcgacg tcgatcagac acgggctcgc gagctgggtc
     3781 tgacgcagca ggacgtgtca aacagtgtgc tggtttcgct atccggcagc ggccaggtgc
     3841 agccgaatta ctgggtcgat ccccggatgg gaatctcgta cctcgtcgag acccgcacgc
     3901 cgacctacaa gctcgacagc gtggatagca tcaatgccct gccgcttagc tccgccgccg
     3961 gcgggccgca gcttttggcg aacgtcgcca cggtcgagcg gagcgtgacg cccgaagtgg
     4021 ccaaccacac caacgtgcag ccgacgttcg atgtctatgc caacgtgcag ggccgcgacc
     4081 tgggcagcgt cgcccgcgag attaaccgcg tgttggccga attccgcacg aagctaggcc
     4141 ccggcgacac gatcgctatg cggggccagg tcgaaagcat gaactcggcg ttcgtccgtc
     4201 tcggcctggg catcgccttt gccgcggtgc tcgtctatct gctcatggtc gtaaactttc
     4261 agagctggct cgacccgttc atcatcatca cggctttgcc cggtgcgttc gtcggcatcg
     4321 tctggtcgct cttcctctgg caaacgacct tcagcgtgcc ggccctgatg ggcgcgatca
     4381 tgtcgatcgg cgtggcgacg gcgaacagca ttctgctggt cacgttcgcc aacgagcaac
     4441 ggggcgaagg cctccctgcg ctggaagccg ccctggaatc cggccgcact cgcctgcgtc
     4501 cggtgctgat gaccgccgcg gcgatgatca tcggcatgct gccgatgtcg ctgggcctcg
     4561 gcgaaggcgg cgaacaaaac gctcctctcg gccgcgccgt catcggcggc ctgctcatcg
     4621 ccacgttgac gaccctgctg ttcgtccccg tcgtctatag cctgctccgc cgcggaagtg
     4681 ccccggctgc cgagtgacaa cgggtggctg tgacagtggt cggccgcggc tgcgccgacc
     4741 actgccaccc gtctgaaaaa tctggaggcg tgtaaggttc gcccgttcgc cctgtatttc
     4801 ctcttgtagg gcacgctaac aaaaacctcc actggagaga cccatgtccg ttgcttccga
     4861 tcaaggcctg attgaatcta aagtctcgct gggccgctcg acgatgaggg tgccggcact
     4921 ggtcgagcgg caagctcgcc gggaactggc gcgcgaaaac gctccgcgcc gcggcctcac
     4981 caaggccgct tgggccgccg gtttcgccgt gctgggcggg atggcgctcg cggctggcgt
     5041 cgaagcatgg cggtccgaac aagctgcgtc cgccgctgtg gaatcgcacg tggagccagc
     5101 gaagacagtc acgatcttac gacccacgcc ggcagcgcat tccagcgtga cgttgccggc
     5161 gacgttccga ccttggcaaa ccgccacgct ccatgcccgc gccagcggct atctaacggc
     5221 ttggcatcgc gacttgggcg ctgtggtgaa ggccggcgag ctgctggccg aaatcgagac
     5281 gccggagctc gaccaggaag tcgccgaagg cgaggcgctg gccagcgagg cggcggcggc
     5341 agccgtgcag gcgcgcgcgg aacggaccga agccgaggcc gagctgaagg tcgtggaagc
     5401 acaactcgcc cgcgtgcaag ccgaatacga actgggaaag tcgcagcttt cccgccgcga
     5461 caagctcgtg cagagccgct cgatatcgca ggaagagctg gatacctatc agcgtcagtt
     5521 gcaggcgcga gccgccgaag tggctgccgc tcaatccgac gtggcccggc ggcggacgaa
     5581 cctgcagacg cgagccgcca tcatcgcggc ccgcgaagcc acggcgaaga gccggcagtc
     5641 gaatgtcgag cggctgaagg agttgcaagc gttcaagcgc atcgtcgctc cgttcgacgg
     5701 cgtcatcacc ggccgcatcg cggaagtggg catgctcgtg acggccggca aggagccgct
     5761 gtttgtgatc gaagacttga gcaagattcg cgtgcagctc agcgtgccgc agacctacgc
     5821 gatgcagacc gcgccgggcg ttgcggccac cattcgtctg ccggaatcgc agacgcagcc
     5881 cgttatggcc gcgatcacgc gaatcgctga gtcggtcgat gctgccagcc gcacgatgct
     5941 tgcggaaatt gagctcgaca acgtcgagca tcactttcag cccggcagct acgctcaggt
     6001 aacgctcgcg gcgccgcaga gcagcagttc gtggtcgatt ccgaccaacg cactgctcat
     6061 gaagaccaac gggccgcacg tggccctggt gaatgaccag aaccaaatcg aggtaaagcg
     6121 agtgacgctc ggccgcgatc taggcgaccg cgtcatcgtg gccgaaggca tccacggcgg
     6181 tgaacggctg gtcgtcaatc cgggtgacgg cgttgcgacg ggcgtgcaag tggtagtgca
     6241 tcagccggcc gatgccgccc gcaaatttgc cgaacgttga ggtttcgtcg aactgtccag
     6301 ccatgcccca taacctgaaa aagctgacat ctcacgccgg ccctcgtccc gccggccacc
     6361 gcggcgtatc attgccggca aggcgggtga caaggaccac gtttgggatc gatcatggcc
     6421 gtggacgaat ccccgctcac gcgagccagc ctgctggtgc aaattcgcga cggggccaac
     6481 cactcggcgt ggcacgaatt cgtgcagctt tacgggccgg ttgtctatgg ctttgcccgc
     6541 aagcggggtc tgcaagacgc cgatgccgcc gatctgatgc aggacgtgct gcggtcggtt
     6601 tcttccgcca ttggccgtct cgactacgac cgccggcaag gcacgtttcg cggctggctg
     6661 ttcacgatca cgcggaacaa gattctcaac tttttcgccg cccgcaaaat ccgcccgcag
     6721 ggatcgggcg atacgacgac caatcgaatg ctcgatgccc agccgagcga cgcgggcgac
     6781 gacgcctggg agctggagta tcagcggcgg ttggccgccc tggcgatgga ccgcatcaag
     6841 ggcgaattcc aggacaagac ctggcaagcc ttctggctga tggcggtcga ggggcagtca
     6901 gcggccgaag tgggccggcg agtcggcctg tcgccggggg cgatctacgt cgccaagagc
     6961 cgcgtgctgg cacggctgaa ggacgaagtc gaagcgctgc ggcagcagga ggaagtctga
     7021 gatgaaaact ccttgcccgg cgacggaaga attgcgcgaa ctgctcggtg gcctggcgcc
     7081 tggcccgcag caagaggtct ggacgcagca tttagatcag tgcgaatgct gccaggcgaa
     7141 gctggaggag ctggccatcg gcgggacgaa cctctcgcag gtcgtccagc agttgcacca
     7201 agccgtgcct gccgcacaga gcgcctactg gccggcgctc cgcgcgatgg aacaagcccc
     7261 cgccctggcg caaacgctcg ctcccgaaac accatcgccc gaggtgaaac agcggctccg
     7321 cgatagctcg acgaacttcc tgttgccgcc gaccgacccg gcgtatctcg gccgcctgga
     7381 gcggttcgac gtaatgcgcg tcattggccg cggcggaatg ggcatcgtgc tcgaagcatt
     7441 cgattcgcgg ctgcagcgga atgtcgccct gaaagtactc gatccggagt tggctagcga
     7501 cgaaacggcc cggcagcgat tctgccgcga ggcccgcgca gccgcgtcga ttacgcacga
     7561 aaacgtcgtg gccgtacacc aggtcgaaaa ggcggccgat aacggcctgc cctatctggt
     7621 gatgcagctc gtggcgggtg agacgctgga gcagcggctc ttgcgcgaga agcggttgcc
     7681 gctaaaggag atcgtccgga tcggcctgca agcggcacag ggattggcag cagcgcacgc
     7741 tcaggggctg acgcaccgcg acgtgaagcc gggcaacatc ctgctggagc cgccgcacga
     7801 caaggtgaag ctcaccgatt tcggcctggc ccgcatcgcc gacgacgtga agctcacccg
     7861 caccggcttc gtcaccggca caccgcttta tatggcgccg gaacaagcgc tgggcgagtc
     7921 ggccgatccg cggtccgact tgttcagctt gggagcgatc ctttacgaga tgtgcgccgg
     7981 acagccgccg tttcagggca actcggcgct ggcgattctg aagcagatcg ccgagtcgaa
     8041 gcaccggccg ctccgcgagc tgaacccgga cgtgcccgag tggctggccg agatggtcga
     8101 tgagctgctg gccaaaaagc cggaggaccg ctatcaaacg gcggccgacc tggccgaggt
     8161 gctcgacttt cactgggctc acatgcgcag ttcgtcggcc gatctgccgg ccgtctgcca
     8221 gacggagttg aagcagcggg ccgctcgcaa ccgctggatc tttgccggcg ttagcgtcgc
     8281 ggtccttgcc gtcggcttgc tggctggcat ggtcttgccg cggctggcga gctggacccg
     8341 tgcgctggct tccgccgcta aacccgccgc ggtgttgccg gccaatgccg gtgcggtttg
     8401 gtcggtggcg tttgatcccc agagcaatac ggtcgcgatg gccgtcgaag atggttccgt
     8461 ccggttgtgg gactggccaa cgaaagccgt gcaatcgacc ttcgaggccc accgcggtgt
     8521 cgtgtgggcg tcgcggtttt cgggcgacgg caaatacatc gccaccgccg gcgacgacgg
     8581 cctgctgaaa atctggaacc gcacgtccct ggaatcgctc caggaattca ggcatcccaa
     8641 cgccgtccgt ggcctggcaa tcggcgcggg cgacaagttc ttcgccggcg accgcaatgg
     8701 gggtctgcat gtctggtcgc tcggccagga caaaccgctc gtcactgcgg agcaacccgg
     8761 agcggtctat acggtcgcca tttcgccgga cggcaaaacg ctggccaccg ccggcagcga
     8821 caaaatcgtc cgcctctggc atgccgagac gctgaagcag cggctgccgc tggaaggcca
     8881 cgccgggccg atctatagcc tgtcgttcaa ccccgagggt agccggctgg cctccgccgg
     8941 ctgggacggc acgattcgca tctgggatcc cgccggcggc aagctgctgc attcgtggga
     9001 ggggcaccgc ggcgacattt gggcgattgc cttctcgccc agcggcacgc ggctggccac
     9061 cggcggccag gacggctcgc tcaagctgtg ggacgctgcc accggccagc tgctggcaac
     9121 ttacctcggc cacaccagca cgatacacac gatcgctttc aacgcagacg gcacactgct
     9181 ggcctccggc ggccgagacg gagccgcacg cgtttggagg attcgttagc ggcagcgggc
     9241 acctagccgg tcgccgccga ccgagtgcga cgatccgcaa tgtcgtcgcc ctggaggccc
     9301 cacgcgggcc tctgtctttt tggttggcca agaggcgagg ctacccgcag atcgccaagg
     9361 ttcggccgac gagggaaaga cgtcggtttt gagtggtatg cggtggtcat gttgcgactg
     9421 ccgtggcgtt cctcaccagc agggccgcac agcgccacct tgtcgctgta gaggatcccc
     9481 agcagcacgg gcgccggccg accgccctgc cgaatcttcc aagctgccct aacggtcggc
     9541 acaggtggct gtttcttcgc tgctgcctgg aaaaagtaat ctcgccgcgg agctacctgg
     9601 cttggcgttc tcgatcttcg acgacccgcc gcgggtcagt tgggagaaca agccggtgga
     9661 aatgaacgcg gacgaggccc tgtccttgga aaacgatggg gcatcgggag agacggctct
     9721 ggacagggca gttggctggc ttcggtgcag gctcgagggc ggacctctgc cggcgaatga
     9781 actgaaggat caagccgagc tcgacggcat tgcgcctcgg acgctcgacc gggcaaaggc
     9841 cgtgctgggc gtgattgtca agccgagtgg attcggtgga ccgcggattt ggtggctgcc
     9901 tggtgacgac agagtgcgcc aagcgaccaa gaggacgcca actaccaaac aatggcgcag
     9961 actggcgatc ctggcgcact ctgtcggacg gctgagctaa gtgaccagca gtgagaccca
    10021 gcgtcgtccg ctggggctca gtgtggtctg cagcgactgg aactgggcac aaggagagca
    10081 atgagcatcg gcaagctcct ccggtcgata tccccagccg acgcagaggc gttccgggtg
    10141 tggctggcaa cggaagcgaa cgaccgcgac aacgggcggg acgagctcag cgataacacc
    10201 gttcgacgcc ggacggggat tgccaagcag ttcttccggg cagcccagaa gcggcacctg
    10261 atcgagtcaa atccattctc caggttggcg gtttgcgagt ggctcgggaa ttcacaggcc
    10321 gttgccctgg aacattatgc cccggtcaca gacgatcatt tccgagccgc cacgggtgag
    10381 ccggtgcgta gagccgaagc aaaaagcgaa gcagaagcga agcagtttcc gaagcagcag
    10441 gcgtccgcag
//
LOCUS       DASRJB010000730        10315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1336906, whole genome shotgun sequence.
ACCESSION   DASRJB010000730 DASRJB010000000
VERSION     DASRJB010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336906"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFB80_19215"
     CDS             <1..276
                     /locus_tag="VFB80_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35972.1"
                     /translation="HRPLYFEDVNLERHGRSHGLAQPAVSAVHFAGRIAAWPYMAGAF
                     PPHECIYTMRHDRPGTYTPYCFYRPPVSLRGAIYEAGAVAGLAYLVP"
     gene            complement(292..2493)
                     /locus_tag="VFB80_19220"
     CDS             complement(292..2493)
                     /locus_tag="VFB80_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35973.1"
                     /translation="MSLATPLTALPTARRDLAASPTLLRGLRYWTIKDPVALRYYQLR
                     EEEHFIWRLLDGRHTLDEIVAEFERRFAPRRLRRSELAGFLMLLHRESLVLSPAWGQG
                     PQLAARRDRQIWQRRLAQLSNVLAIQLPGINPDRLLTVLAPRLRWIFSAWCGAAAVAL
                     VVAAGILAALHFGALVERLPHVRDFLGPANWLWLAAALALVKSLHELGHAVACKHFGG
                     ECNRLGLMLLIFTPALYCDVSDSWMFTSRWRRIAVSGAGMAVELVLAALALILWSASE
                     PGWFNSLCLNIVFVCSVGTLLINGNPMLRYDGYYILADLLGAPNLRQQASAAIGRGLV
                     RLLTGVEVEQPRLLAEPGWAVLWAYGLGSAAYRVLVLVAVLWFVHSVLASHGLAVVAQ
                     AIVVLTLAAVTVPPAVGGVRWLSDPARRKQIKPWRVGVAILGLIALAAAVLLVPLPSR
                     ITAPVVLRPEGARRVYVTTPGRVVSTLAAGSQVTAGQEVARLQNPALERQVVALRGRC
                     EAQRVHLRQLELRRHDDESLGDQLPAAARLLADLQTQLAQQERELARLTLTAPTSGTV
                     LPPPPRPDRPAAGELAEFAGTPLDEPNRGCRLESGTLVCLVGDPARLEGVAIVDESAV
                     QRLRVGQRVRLQLAELPGKVLHGQVTEVSRLNSGELPDEIVARGMLPLERRPDGGIRS
                     VRTYYEAVVVVDQHTAPLLINATGWARIEAAPQPLWLHIYRALRSTFRLAW"
     gene            complement(2520..3416)
                     /locus_tag="VFB80_19225"
     CDS             complement(2520..3416)
                     /locus_tag="VFB80_19225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN35974.1"
                     /translation="MSRWKFALRVLAVCTGSIGLAVRGWASDIAVESVVINLVADVEV
                     PANEAGPLVELSVSELSRVEAGAVLARIDDRDAALELARARIAVEHAEHLAANDVKIR
                     QGRQALKLAELELKRAEDANREVSGVVSATQIEKLKIGIENAQLEIEQATRDQAAAQR
                     AVEAAKNDLQLAQRAVERRRIVAPIAGVVVDMRKRAGEWLKPGDTVLRIVRDDRLRAE
                     GFIDVQQLTASFLGEPAELTVALPGGKSAIVRGKVTFVSPEANPINRQVRVWAEFENQ
                     DGRLRPGLSAQMVIRPTPGSAQ"
     gene            complement(3446..5113)
                     /locus_tag="VFB80_19230"
     CDS             complement(3446..5113)
                     /locus_tag="VFB80_19230"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN35975.1"
                     /translation="MPNPSNRPLILRPVQCEESATYLLAVALDAAAPRAADSAAAQVL
                     NLFCETAAEFHQRRELQERRRLHTNLREFDQLVARLHANLDLDKTAYALANDGRLWIG
                     CDRLSVGLIHGQSVRVRAISGSDQVDRRSQQVGSLEDLLAAVAASGEPLHWPGESKGG
                     ELPPQLDTPLQAYLDLAHARRLIVAPLRAQAAAESRQDAGPPIGVLLAETFDDVQSTE
                     RLRQRVDDVVRHGGAALANSIAFHELPLRPLQAGMAQLLRSLRRRPVPALMAAVCAAA
                     VAAALAFIPAEFSVPALGTLQPEGRRHLFAPASGVVERVAVAHGQRVAPGDELLRIRD
                     PELDLDYSRTSGELQTHQARLASIRARRSTRSTATDPQQEDRQLAAEEEQLKQQLSSL
                     AAQLELLERRHAELVVTSPITGIVLTWNPEELLRDRPVREGQMLMTVADPAGPWILEL
                     RVPDTDIGPLLAAQRQGPSELPVTFLLETEPAATYRGRIAKVAEASDSAGGASPSVLV
                     TVQLESQTPASARAGAGVVARIHCGPKSIGYVWLHDLIDAVRTQLLF"
     gene            5652..7676
                     /locus_tag="VFB80_19235"
     CDS             5652..7676
                     /locus_tag="VFB80_19235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN35976.1"
                     /translation="MALLLAGTLCACGCRTHEPNVAIDPHVAYFQSQAREVEYPDVVA
                     PPSVETLDTPPPWLLSSGGPPPFREITLQEAVQTALARSPVLRDVGGLVLQTPGALQT
                     TFTPAITETDPRFGVDAALSAFDAQLSAGAYFEKNDRAFNNAIAGLGTQTFRQDLNNY
                     NIELRKRTAAGTILAARGRFDYDFNNSPFNNVPNLPWTMRLEGELRQPLLQGAGVDFN
                     RIAGPANIPGFYNGVLISRINTDISTADFEAAVVQLVSQVENAYWELYFAYRDLDAKI
                     LARQQAFETWQQVSVLFRRGPGGQLEATAEAQAREQLYRLEAEVQDAKVGKQQEKSNT
                     NIFRGTGGVYASERRLRRVMGIPASDGTLLRPANEPTIARTLFVWEEVLSESLVRRVE
                     LRRQKWQVKRRELELIAARNYLLPRLDAVGLYRFRGLGHDLVGNADQQFGSALSDLVT
                     GDFQEWALGVEMTAPVGFRQGHAAVRNAELQVSREQAVLDEQERQVVADLSSVVGELD
                     RAYELARTSFNRKMAAQRQLDTTSALLLQADDIDKPRLLDLKLDAQRRLADAESQYHR
                     SLAEHEVAIKNMHLEKGSLLEYNRIYLSEGMWPGKAYRDAARREKLRITLPRLDNYVR
                     DDRIVSQGPYAQEFLPPTGEELPPSQPKPAEGAAPEPLLPAPLPAPLSSE"
     gene            8009..>10315
                     /locus_tag="VFB80_19240"
     CDS             8009..>10315
                     /locus_tag="VFB80_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35977.1"
                     /translation="MTQFNGAFYFAAEGTNSSGQNVGRELFRLNADGSTELVADINPG
                     AASSAPGDFAFFSPNGSDRLLFAATGLSGREIYQLDTSGNVTLVADVNPGLASSDPVM
                     LTEFSGKLYFSATTSATGREIYVVNNGGSVSLVADINPGSASSNPSAFTAFSSNLYFT
                     ADGPGGRHLYRESPSGTSDPVLVNLGAGVLDPQGFTEFGGKLYFSALDPVDGRELFVL
                     TPSGNNKSETVTKVANLDGTSASSSPGEFFVFDNRLYFAATAANGRELFRLDSSGQIT
                     QIDINAGPASSSPSGFTLFDGQLYFAATVGGQRGLFRLSPGAASPVPVSLPSGVMLPQ
                     DVVFYAVEDVLFFAAEGPLGRELYRMGPGEIVTLAADVNSGSASSDPAEVIALAGHIY
                     FVASDVTVGRELFVLRQNPSSIRIVGDELVFTDEDGDEDNRLVVSSDGTNLKIRDENG
                     HTIFLFTAIAGATGGGTNEVTIPFSSLAGINALAIDTRGGNDSVTFDLSANADAILGQ
                     FATSTYDGGPNSPALPGDRLRFIGDGLTRSQYTPDALVTGSGVVLVSSATHSTSFGFL
                     HLEPVDFTGMSEARLVTPGTGTGSDALVVSPGLDGRDGLISALIVSGTVGGTTIESAH
                     FFGNQTVVIATGSGLDGTDSVTVNGAANGHNNSNLSIDTGTAGQDSVLILGDVTLAGN
                     LLITTNALDVRALLSIGGTAAFATRDDIVFSSAGRLLAPVAALTAGDQIALAAGSLVD
                     AGSGTISLTATGDITLGRLVTTNGTNQAV"
BASE COUNT         1819 a         3451 c         3290 g         1755 t
ORIGIN      
        1 caccggccgc tctatttcga ggacgtcaac ctcgaacgcc acggccgcag ccatggcctg
       61 gcccaaccgg cggtttcggc ggtccacttt gccggccgaa tcgccgcctg gccgtatatg
      121 gcgggcgcct ttccgccgca cgagtgcatc tacaccatga ggcatgatcg gccgggcact
      181 tacacgccgt actgcttcta ccggccgccg gtcagcctgc gcggtgcgat ttacgaagcg
      241 ggcgccgtcg cgggtcttgc ttacctcgtt ccctgacgga cgcagcgagt ctcaccatgc
      301 caggcggaat gtactccgca gggcacgata tatatggagc cagagcggct gcggcgccgc
      361 ttcgatccgg gcccagcccg tcgcgttgat aagcagcggc gcggtgtgct gatcaacgac
      421 aacaaccgcc tcgtagtagg ttcgcacgct gcggatgccg ccgtcgggcc ggcgctccag
      481 aggcaacatt ccgcgggcga cgatctcgtc tggcagctca ccggaattca atcgcgagac
      541 ttcggtcact tgtccgtgga ggacttttcc cggcagctcg gctagctgca agcgcacccg
      601 ctgaccaacc cgcagccgct ggacagccga ttcatcgacg atcgccacac cttccagccg
      661 cgccggatcg ccgaccaggc agacgagcgt tcccgactcc agcctgcagc cacggttcgg
      721 ctcgtcgagc ggcgtgcccg cgaattctgc taattcgccg gccgccgggc gatcgggccg
      781 cggaggcggt ggcagcaccg tgccgctggt gggcgcggtc agcgttagcc gcgccagctc
      841 gcgttcttgc tgagcaagtt gcgtctgcag gtcagctagc agcctggctg cggctggaag
      901 ctggtccccc aggctctcat cgtcgtgccg gcgcagctcc agctggcgga ggtgaacgcg
      961 ctgcgcctcg cagcgtcccc ggagcgcgac tacctgccgc tccagcgcgg gattttgcaa
     1021 gcgggccact tcttggcccg ccgtcacctg cgagccggcc gcgagagttg aaacgacccg
     1081 gcccggcgtc gtgacgtaga cccgccgagc gccctcgggc cggagcacga ctggcgcggt
     1141 aatccgggaa ggcagcggca ccagcaggac cgcagccgcc agcgcgatca gcccgagtat
     1201 ggcaacgccc acacgccacg gcttgatctg cttccgccgc gccggatcgc tgagccagcg
     1261 cactccgccg accgcgggcg ggacggtaac agccgccagc gttaacacga cgatcgcctg
     1321 cgcaacgacg gccaggccgt gtgaggctaa caccgagtgc acaaaccaca gaaccgcgac
     1381 gagcaccagc acgcgataag ccgctgagcc caggccatac gcccagagca cggcccagcc
     1441 gggttcggcg agcaatcgcg gctgctccac ctccacgcct gtcagcaggc ggacgagccc
     1501 gcggccgatc gccgcagaag cctgctgccg cagatttgga gctcccagca ggtcggcgag
     1561 aatgtagtag ccgtcgtagc gcagcatcgg attgccgttg attagcagcg tgcccaccga
     1621 gcaaacgaat acgatgttga ggcacagcga attgaaccag ccgggctcgc tggccgacca
     1681 caggatcaga gccagcgcgg ccagaaccag ttccacggcc atgcccgctc cgctgacggc
     1741 gatgcgccgc cagcggctgg taaacatcca cgagtcggaa acgtcgcaat agagcgctgg
     1801 cgtgaaaatc aggagcatca gtcccagccg attgcactcg ccgccaaagt gtttgcaggc
     1861 cacggcgtgg cccagttcgt gcagcgactt caccaaggcc agagcggcgg ccagccacag
     1921 ccaattggcg ggccctagaa aatcgcgaac gtgcggcaag cgctcgacca gtgcaccgaa
     1981 atggagggcc gccagaattc ctgccgctac gaccagcgcc actgcggccg cgccgcacca
     2041 cgcggagaaa atccacctta gccgcggcgc gaggaccgtc agcaatcgat cgggattgat
     2101 ccccgggagc tgtatcgcca gcacgttgct cagctgggcc agccgccgct gccagatttg
     2161 gcggtcgcgc ctggccgcga gctgcggccc ctgtccccag gctgggctaa gcacgaggct
     2221 ctcgcggtgc agcagcatca ggaatccggc cagctcgctg cgccttagcc tgcggggggc
     2281 gaaacgccgc tcgaactcgg ccacgatttc atccagcgtg tgccgcccat cgagcagccg
     2341 ccagatgaag tgctcttcct cgcgcagctg atagtaccgc agggcgactg ggtccttgat
     2401 cgtccagtag cgcagcccgc ggaggagcgt cggcgacgcc gcgaggtcgc ggcgcgccgt
     2461 gggcagcgcg gtcagcggtg tcgcgaggga catgaatcga cgcgaaagct ccagctgggc
     2521 tactgagcgc tgcctggggt gggccgaatg accatctgag cggagagccc gggacggagc
     2581 cggccgtcct ggttttcgaa ctccgcccag acgcggacct ggcgattgat cgggttggct
     2641 tccggcgaga cgaaggtcac tttgccgcgc acgatggcgg acttgccgcc cggcagcgcg
     2701 accgtgagct cggccggctc gcccaggaag gatgccgtga gctgctgcac gtcgatgaaa
     2761 ccttcggcac gcagccggtc gtcgcgaacg atgcggagga cggtgtcgcc cggcttgagc
     2821 cattcgccgg cccgcttgcg catatcgacg acgaccccgg cgatgggagc cacgatgcgc
     2881 cgccgctcga cggcgcgctg ggcgagctgc aggtcgttct tcgccgcttc gacggcccgc
     2941 tgggcggccg cctggtcgcg cgtggcctgc tcgatctcga gctgcgcgtt ttcgattcca
     3001 attttcagct tttcgatctg cgtggccgag acgacgccgc tcacctcgcg gttggcgtct
     3061 tccgctcgtt tgagctccag ttcggccagc ttgagcgctt gcctgccctg ccggattttg
     3121 acgtcgttgg cggcgagatg ctcggcgtgc tccaccgcaa tccgggcgcg ggccaattcc
     3181 agcgcggcgt cgcggtcgtc gattctcgcc agcaccgcac ccgcctcgac gcgcgacagc
     3241 tcggagacac tcagttcgac cagcgggccc gcttcgttcg cgggaacctc cacgtcggca
     3301 acgaggttga ttacaaccga ctcgacggca atgtcgctgg cccatccgcg taccgccagc
     3361 ccgatggacc cggtgcagac tgccaaaacc ctgagggcga atttccagcg actcatgagt
     3421 ggctccgatc gccaacctgg tgtgcttaga acaagagctg cgtgcggacg gcgtcgatca
     3481 ggtcgtgcag ccagacatag ccgatcgact tagggccgca gtggatgcgg gcaaccaccc
     3541 ccgcgcccgc ccgggcgctg gccggcgtct ggctttccaa ttgcacggta accagcaccg
     3601 acggcgaagc gccgccggcg gaatcgctgg cctcggcaac cttggcaatt cgcccgcgat
     3661 aggtcgctgc gggctccgtc tccagcaaaa acgtgacggg cagttcggac ggtccttgcc
     3721 gttgtgcggc cagcaggggc ccaatatccg tatcaggaac gcgtagctcc aagatccacg
     3781 gcccggccgg atcggccacg gtcatcagca tttggccttc ccggacgggg cgatccctga
     3841 gcagttcctc cggattccac gtcagcacga tgcccgtgat cgggctggtg accaccaact
     3901 cggcgtgacg gcgctccagc aactcgagtt gggcggccag gctgctgagc tgttgcttga
     3961 gttgttcttc ttccgccgcc agttggcgat cttcttgctg cggatcggtg gccgtgctgc
     4021 gcgtggacct gcgcgctcga atcgacgcca gccgtgcctg gtgagtctgc aattcgccgc
     4081 tggtgcggct gtagtcgaga tcgagctcag gatcgcgaat ccgcagcaat tcgtcccccg
     4141 gggcaacgcg ctggccatgt gcgaccgcca ctcgctcgac cacgccactg gcaggcgcga
     4201 acaaatgccg ccggccttct ggctggagtg tgcctaaggc tggaacggaa aactcggcgg
     4261 gaataaaggc caaggcggcc gccaccgccg ccgcgcaaac tgccgccata agtgctggga
     4321 ccggccgccg tcgcaacgat cgcagcagct gcgccatgcc cgcctgcaac ggccttagcg
     4381 gcagttcgtg aaaggcaatc gaattggcca gggccgcgcc gccgtgacga acaacatcgt
     4441 ccacgcgctg gcgaagtcgc tcggtcgatt gcacatcgtc gaaggtctcg gccagcaata
     4501 cgccgattgg tggtccggcg tcctgccttg actcggccgc tgcttgcgcc cgcagcgggg
     4561 cgacgatcag ccggcgggcg tgggcgagat cgagataagc ctggagcggc gtatcgagct
     4621 ggggcggcaa ttcgccgcct ttcgattcgc cgggccagtg cagcggctct ccactcgcgg
     4681 cgacagccgc cagcaagtct tccaggctgc cgacctgctg agaccgtcga tcgacctggt
     4741 cgctgccgct gatcgctcgc acgcggacac tttggccatg gatcaggccg acgctcaagc
     4801 ggtcgcagcc gatccacaag cggccgtcgt tggccagggc ataggcggtc ttgtccaggt
     4861 cgaggttggc atgcagccgg gcaacgagct gatcaaattc gcgaaggttc gtgtgcagcc
     4921 gccgccgctc ctgaagttca cgccgctgat ggaattcggc cgctgtttcg caaaacagat
     4981 ttagtacctg cgccgcagcg gagtctgctg cgcgcggagc agcggcgtcg agcgctaccg
     5041 cgagcagata ggtcgccgac tcctcgcact gaaccgggcg gaggatgagc gggcgattcg
     5101 atggattggg cacagccgat cgctcggacg tggaatgggg cggcagcacc aaacaagatt
     5161 ccgcaatttc tccggccaat cgccaacctt ccacccagcg aaccatctcg tcggaaatgg
     5221 ccggggccgc acccgcttgc gcaactcgcg cgaccggcat tgccggtact atttgccaaa
     5281 ccgcgccgcc atcggccttc agcgcacgca gcaagccgtc aagcagcgcg ccataaaacg
     5341 ctggaacttc aacgggcgac ctggccaaat tggccatttc gtccagcagg ccatcaattt
     5401 cctgccacgc ctctgtgtag gggccgatgg gcaggtgcag agattgtgag actgggcctg
     5461 ccattgggct ggcaacctgt tgccgttttg acgatttgac tcggcgggcg tgtcaaattg
     5521 aattgatctt gaattggcga ctttgccgat ttcgccaatg aagcctatta gaacgccctc
     5581 cgcgatctgc ccgcctgctc gcttcaaccg gcttgcccca ggctaccatg cgcccagcgg
     5641 ttcggaaact catggcactg cttttggcgg gcacgctctg cgcctgcggc tgccgcacgc
     5701 acgagcctaa cgtcgcaatt gatccgcacg tggcctattt ccagagccag gcccgcgagg
     5761 ttgaatatcc ggatgtcgtc gctccgccgt ctgtggagac gctcgatacg cctcctccct
     5821 ggctgctcag cagcggggga cctccgccgt ttcgggaaat aacgctgcaa gaggcggttc
     5881 agaccgctct ggcccgctcg cccgtgctgc gcgacgtcgg gggcctggtg ctgcaaacgc
     5941 cgggggcgct gcaaacgacg tttacaccgg cgattaccga aacagatccg cggttcggcg
     6001 tcgatgccgc gctgagcgcg ttcgacgcgc agctttcggc cggcgcttac tttgagaaga
     6061 acgatcgggc cttcaacaac gcaatcgcgg ggctcggcac ccagacattt aggcaggacc
     6121 tcaacaacta caatatcgaa cttcgcaagc gcactgcggc cggcacgatt ctcgcagctc
     6181 gcggcaggtt cgactatgac ttcaataact ccccgttcaa caacgttccc aatttgccct
     6241 ggacgatgcg cctggagggc gagctgcgcc agccgctgct gcaaggcgcg ggcgtggatt
     6301 tcaaccggat cgccggcccc gcgaatattc ccggcttcta caacggcgtg ctgatctcgc
     6361 ggatcaacac ggacatctcg acggccgatt tcgaggccgc cgttgtgcag ctggtctcgc
     6421 aggtggagaa cgcttactgg gagctgtatt tcgcctatcg cgacctggac gccaaaattc
     6481 tggcgcgaca gcaggcgttt gaaacctggc aacaggtcag cgtgttgttc cggcgcggac
     6541 cgggcggcca gctcgaagcc accgccgagg ctcaggcccg cgaacagctc tatcgcctgg
     6601 aggccgaagt ccaggacgcc aaggtcggca agcagcagga gaaatcgaac accaacatct
     6661 ttcgcggcac gggcggcgtg tatgcttccg agcgacggct gcggcgggtc atggggattc
     6721 ccgccagcga cggcacgctg ctgcggccgg cgaacgagcc cacgatcgcc cgcaccttgt
     6781 tcgtgtggga agaggtgctg agcgagtcgc tggtgcgccg cgtggagctc cgccgccaga
     6841 agtggcaagt gaagcgccgc gaactggagc tgattgccgc tcgcaactac cttttgccgc
     6901 ggctggatgc cgtgggactc taccgcttcc gcggcctggg acatgacctg gtcgggaacg
     6961 ccgatcagca gttcggcagc gcgctttccg atctggtgac cggcgacttt caagagtggg
     7021 ccctgggagt cgaaatgacg gcccccgtgg gctttcgcca aggccacgcc gcggttcgca
     7081 atgccgaatt gcaagtgtct cgcgagcaag ccgtgctcga cgagcaggag cggcaagtcg
     7141 tggccgatct cagcagcgtc gtcggggagc tcgaccgcgc gtatgaattg gcccggacca
     7201 gtttcaaccg gaaaatggcg gcgcagcgcc agctggacac tactagcgcg ctattgctgc
     7261 aggccgacga catcgacaag ccacgactgc tcgacctgaa gctcgacgcc cagcggcggc
     7321 tggcggatgc tgagagccag tatcaccgca gcctcgctga gcacgaggtg gccatcaaga
     7381 acatgcatct cgagaaaggc tcgctgctgg aatacaaccg gatttacctc tcggagggga
     7441 tgtggccagg caaagcctat cgcgacgcgg cacgccgcga gaagttgcgg atcactcttc
     7501 cgcggctgga caactacgtt cgagacgatc ggattgttag tcagggcccc tatgcccagg
     7561 agtttctccc gcccaccgga gaagagctcc cgccatcgca gccgaagccc gccgaaggcg
     7621 cagctcctga accccttctc ccggcaccgc tacccgcgcc gctatcttcg gaatgatcgc
     7681 gccaatctgg cgccgatact taccagccgt ttgacagttc cctccgacat aacgacaatc
     7741 gggaactggt cgtttctgcg gcaggtctgc gaaaacccgc cgggctctga tctctctccg
     7801 gtgagtttcc aggcaggaat taatgccgat cagcaggttt ttcaagcagt ggcgcaccgt
     7861 acggcatcgt acgcgccgcg tgcctggagc ggccgagtcc ggctacgtgc gactcggcct
     7921 gacgcggttg gaagatcgtg ttgtattgga cggcgccggc gtcgttacgg atatcaatcc
     7981 gggctcggca agctcatcgc cgacggaatt gacgcagttc aatggcgctt tctacttcgc
     8041 ggccgagggg acgaactcca gcggccagaa tgtcggccgc gaattgttcc gcctcaacgc
     8101 cgacggcagc acagagctgg tggccgacat caatcccggt gcggccagct cggctccggg
     8161 cgacttcgct ttcttcagcc ccaacggcag cgatcggctg ctatttgcgg cgaccggact
     8221 tagcgggcgc gagatctatc aactggacac cagcggcaac gtcacgctgg tcgccgacgt
     8281 caatccgggc ctggccagct ccgacccggt catgctcacg gagttcagcg gcaagctgta
     8341 tttctcggcg acgacgagcg caactggccg cgagatctac gtcgtcaaca acggcggcag
     8401 cgtgtcgctg gtggccgaca tcaatcccgg ctcggccagc tcgaacccga gcgcctttac
     8461 cgcattcagt agcaacctgt acttcacggc ggacggcccc ggcggccgtc acctttatcg
     8521 ggaaagcccc agcggcacct ccgaccccgt gctggtgaat ctcggcgcag gagtgctcga
     8581 tccgcagggc ttcaccgaat ttgggggcaa gctctatttc agcgcgctgg acccagtgga
     8641 cggccgcgag ctctttgtcc tgacgccgtc aggcaacaac aagtctgaga cggtcaccaa
     8701 ggtggccaac ctcgacggca cgtcggccag ctcgtcgccc ggcgagttct tcgttttcga
     8761 caaccggctg tacttcgccg cgaccgccgc caacgggcgg gagctgtttc gactggacag
     8821 ctccggccag attacgcaga tcgacattaa cgcaggaccg gccagctcgt cgccctccgg
     8881 tttcaccctg ttcgatgggc aactctattt tgcagccacg gtgggcggcc agcggggact
     8941 gttccgcctg agtcccggcg ctgcctcgcc ggtgcccgtg tccctgccga gcggcgttat
     9001 gctgccgcag gacgtcgtat tttacgcggt tgaagacgtc ctgtttttcg ccgccgaggg
     9061 gccgctgggc cgcgaactct accgcatggg tcccggcgag atcgtcacgc tggcggcgga
     9121 tgtcaatagc ggcagcgcca gctccgatcc ggccgaagtg atcgcgctgg ctgggcacat
     9181 ctatttcgtg gcgagcgacg tcacggtggg ccgcgagctg ttcgtgctgc gccagaatcc
     9241 ttcgtcgatt cgcatcgtgg gcgacgaact cgtcttcacc gacgaggacg gcgacgaaga
     9301 caaccgcctg gtagtttcca gcgacggcac gaacctgaag attcgcgacg agaacggcca
     9361 caccattttc ctgttcacgg cgattgccgg tgcgaccggt ggcgggacca acgaagtcac
     9421 gattccattt agcagcctgg cgggaatcaa cgccctggcc atcgacacgc gaggcggaaa
     9481 cgactcggtg acgttcgacc tgtccgccaa cgccgacgcc atcctcggcc aattcgcgac
     9541 ctcaacgtac gatggcggcc ccaattcacc ggccctgccc ggcgaccgcc tgaggttcat
     9601 cggcgacggc ctcacgcggt cgcagtacac gcccgacgct ctggtcaccg gcagcggcgt
     9661 cgtcctcgtc agttcggcta cacactcaac gagtttcggc ttcctgcatc tggagccggt
     9721 cgatttcacg ggcatgtcgg aggccaggct cgtcacgcca ggcacgggca caggcagcga
     9781 cgccctcgtc gtctctcccg gcctcgacgg gcgcgacggt ctgattagcg cactgattgt
     9841 ctctgggacg gtcggcggca cgacaatcga gagcgcccat ttcttcggca accagaccgt
     9901 cgtgattgcc acgggcagcg gcctcgatgg taccgactcg gtgaccgtaa acggcgcagc
     9961 caatgggcac aacaactcga atctatcgat cgacaccggc acggctggtc aggattcggt
    10021 gctgatcctg ggcgatgtga cgttggcggg aaacctgctg attacgacga acgcgctcga
    10081 cgtgcgggcg ctgctctcca tcggaggaac cgcggcgttt gcgacgcgcg acgacattgt
    10141 cttcagctcg gccggccggc tgctggcgcc cgtcgccgcc ctcaccgccg gcgatcaaat
    10201 cgccctggct gcaggctcgc tcgtggatgc gggcagtggc acgatcagcc tgactgctac
    10261 cggcgacatc acgttgggcc gactcgtgac caccaatggc acgaaccagg ccgtc
//
LOCUS       DASRJB010000731         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1625019, whole genome shotgun sequence.
ACCESSION   DASRJB010000731 DASRJB010000000
VERSION     DASRJB010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625019"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..993)
                     /locus_tag="VFB80_19245"
     CDS             complement(253..993)
                     /locus_tag="VFB80_19245"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZN35978.1"
                     /translation="MKMSDSDLRRLTRRRLLRGALCTGLSLGACGWAIAQDGPNSSLY
                     SGQFPVMSQQAPGKAGAVPQQPLGWYEVPLPPPKEVRIHDIISIRVDMGSRVSTSAQL
                     QNRRTAQYDARLNDWIFLEGLRAVKPAPQSDGDQRVQGNLTNLNRVTGQLDTTENLKF
                     EIAATVAAVLPNGNIVLEAHESVRVNDEQWMQSLSGVCRREDIGPGNVVLSKDIANLK
                     VDKRDFGQIRDTYKRGWLTRLWDQFTPF"
     gene            complement(990..1631)
                     /locus_tag="VFB80_19250"
     CDS             complement(990..1631)
                     /locus_tag="VFB80_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HZN35979.1"
                     /translation="MYVVPLLGVLLLAGVSSAAEVRLRPTATCGSTVVRLADVAEIEA
                     ADASLAAALGEIPLCPAPAVGGERSLSQHDVRQLLVLSGVERADVNVGGSERVTIQAE
                     KSLMHPAPQAGAAGVRQAAFQTQAKKAQPAAPEPPADKLAKREQLVDRGANVSVYARA
                     AGVRIATSGKALQPGAAGETILVEMENKEKVQARVVAAQAVEVAVPGKNGVSR"
     gene            complement(1679..2485)
                     /gene="flgG"
                     /locus_tag="VFB80_19255"
     CDS             complement(1679..2485)
                     /gene="flgG"
                     /locus_tag="VFB80_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZN35980.1"
                     /translation="MSVQSLYTAATGMESLQSKLNVIANNLANVNTTGFKSDRANFED
                     LFYRHEIFPGSQDQANNFTATGTQIGLGVRMQSTQTQFQQGAFQETGGPLDLAIEGKG
                     FLQVVDPVTSQNLYTRAGNLSLNAQGTLVVGSAQTGRVVVPQIQIPNDANGISISSDG
                     RVFFQQAGNPQMQPAGQLQLATFFNPEGLLKIGENLYSETISSGSPQQNNPGTQGAGV
                     LRQGFLELSNVEPVRELIDLITTQRSFEMNSQVIDAGNQILQVITNLARN"
     gene            complement(2493..3248)
                     /locus_tag="VFB80_19260"
     CDS             complement(2493..3248)
                     /locus_tag="VFB80_19260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HZN35981.1"
                     /translation="MYYGLYMSAAGAHSQSQKVQVLSNNIANGATVGFKRELALLESR
                     ESEAIEQGLARRGSRTVEDVGGGAALRATATDFAPGDLQKTDNPTDFAIEGDVPDVFF
                     LVQRGSERLLTRAGNFQLSNEGMLQTSGGDSVLNSDGDPIQIDPLREWRVVPGGIIDQ
                     AGSRVELALVRPDKLNDLVKVGHNYFSSKSSRPVEAGDRRVRGGFRELSAIKPVQEMV
                     ELIAASRAHEANVRLIQQHDQATSQLINRILRV"
     gene            complement(3446..4375)
                     /gene="hemC"
                     /locus_tag="VFB80_19265"
     CDS             complement(3446..4375)
                     /gene="hemC"
                     /locus_tag="VFB80_19265"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN35982.1"
                     /translation="MAQSQEKLRLGTRASPLARWQAEWVAARLAELGTPVELVPITTQ
                     GDVKSGPLGQIGGQGLFTKELQRALLDEQIDLAVHSLKDLPTAPVEGLIIAAVPPRES
                     TADVLVSAKAGSIETLPHAARVGTGSLRRKAQLLYLRPDLVIEDIRGNLDTRLRKLDE
                     GAYDAIILAEAGLKRLGLASRIRQVIPRSTMLSAVGQGALGIETRAGDVRSRSLVAPL
                     DHEPTHLAVVAERTLLSTLRAGCLAPVGALATASGEQLSLTAVVLSPNGKHRLAAGAA
                     MTVSDAIYLGQAVAEELLSQGAAELIAGARQGQ"
     gene            complement(4430..>5044)
                     /locus_tag="VFB80_19270"
     CDS             complement(4430..>5044)
                     /locus_tag="VFB80_19270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZN35983.1"
                     /translation="FAVSSRQRCSRCGLICAETDLPRGDCGYCRGQRLHFTAVRTLGS
                     YQDALRQAVLKIKHAAYEPLTVALGQRMAACLLARPLAEMPDLVAPVPMHWLKRLVRR
                     THTAETLARAVATTLGLPLQSRLIRCRRLLARQASLTPPQRKLNVRDAFRASPWTRLN
                     GRRVLVVDDVMTTGATAQEVSRALREAGAAAVYVATVARSTPDF"
BASE COUNT          805 a         1671 c         1643 g          925 t
ORIGIN      
        1 cgaccaggcc catgccgtgc agcacgttct cttcctgccc cttgatccgc gccacgtcgc
       61 cgatgcggtg ataggccagc gcgggcgcgg ccacgcagaa caaaatcaga atggccgcgg
      121 cgaggcagct tcgtttgaat ggcatgaggt cctccgtgcg gctttgattt cagattctaa
      181 atttcaaatc tggcaattga gcgaatcgag ccttcttcaa atctgaaatc tgcaatccta
      241 aatctgaaat ccctaaaacg gcgtaaactg gtcccacagc cgcgtcagcc agccgcgttt
      301 gtaggtgtcg cgaatctggc cgaagtcgcg cttatcgacc ttgaggttgg cgatgtcctt
      361 gctgagcacg acgttgcccg ggccgatgtc ttcgcggcgg cagacgcccg acagcgactg
      421 catccactgc tcgtcgttga cccgcacgct ctcgtgggcc tccagcacga tgttgccgtt
      481 gggcagcacg gcggcgaccg tggcggcgat ctcaaacttc aggttctccg tcgtatcgag
      541 ctggcccgtc acgcggttca ggttggtgag gttgccttgc acccgctggt cgccatccga
      601 ttgcggcgcg ggcttcaccg cccgcagccc ttccagaaag atccagtcgt tcagtcgggc
      661 gtcgtactga gccgtgcggc ggttctgcag ctgggcgctg gtcgagacac gcgagcccat
      721 atcgacgcgg atcgagatga tgtcgtggat ccgcacttcc ttcggcggcg gcagcggcac
      781 ctcgtaccag cccagaggct gctgcgggac ggcgcccgcc ttgcctgggg cttgctgcga
      841 catcacgggg aactggccgc tgtagaggct cgagttcggg ccgtcctggg caatcgccca
      901 accgcaggcg ccgagcgaca ggcccgtgca gagcgccccg cgcagcagcc tgcggcgcgt
      961 cagccggcgg aggtccgagt cgctcatttt catcgcgata ctccgttctt acccggcacg
     1021 gcgacttcca ccgcctgggc ggcgacgacg cgggcctgga ccttttcctt gttttccatc
     1081 tccaccagga tcgtttcgcc cgctgcgcct ggctgcagcg ccttgccgct ggtggcaatc
     1141 cgcacaccgg cagcgcgggc atacacgctg acgtttgcgc cgcgatcgac cagctgctcg
     1201 cgttttgcca gcttgtcggc tggcggctcc ggcgctgccg gctgggcctt tttggcctgc
     1261 gtctggaagg cagcctggcg gacgcccgcg gcgccggctt gcggcgcggg atgcatcaac
     1321 gacttttccg cctggatggt aactcgctcg ctgccgccga cattgacatc cgcacgctcg
     1381 acaccggaaa gcaccagcag ctgccgcacg tcgtgctgcg agagcgaccg ctcgccgccc
     1441 acggccggcg caggacaaag cggaatctcg cccagcgcgg ccgccaacga cgcatctgct
     1501 gcctctattt cggccacatc ggccagacgc actacggtag agccgcaagt ggccgttggc
     1561 cgcagtcgca cttcagcggc gctggaaaca ccggccagca ggagcactcc cagcagcggt
     1621 acgacgtaca tcgacctctc ccacaagtga ccaaaaacaa gtgacaagtg accttcacct
     1681 agttccgcgc caaattcgta atcacctgca aaatctggtt ccccgcgtca attacctgcg
     1741 agttcatctc gaacgagcgc tgcgtcgtga tcaggtcgat cagctcgcgg accggttcga
     1801 cgttggagag ttccaggaat ccctgccgca gcacgcctgc gccttgggtg ccggggttgt
     1861 tctgctgagg cgagcccgac gaaatggttt cggaatacag gttctcgccg atcttcagca
     1921 gtccttcggg gttgaaaaag gtggccagct gaagttgccc cgccggctgc atctgcggat
     1981 tgccggcctg ctgaaagaac acgcggccgt cggacgaaat ggaaatgccg ttggcgtcgt
     2041 tgggaatctg gatttgcggc accaccacgc ggcccgtttg tgccgagccg acgacgagcg
     2101 tgccttgtgc gttgaggctg aggttgcccg cacgggtgta gagattctgg ctggtgaccg
     2161 gatcgacgac ttgcaggaat cccttgccct cgatggccag gtcgagcggc ccgcccgttt
     2221 cctggaaggc cccttgctgg aactgcgttt gcgtgctctg catccgcact ccgaggccga
     2281 tctgcgtgcc cgtcgccgtg aagttgttgg cctggtcctg cgaaccggga aaaatctcgt
     2341 gccggtaaaa caggtcctcg aagttcgccc ggtccgactt gaagccggtg gtgttgacgt
     2401 tcgccaggtt gttggcgatc acgttgagct tcgactggag cgactccatg ccggtcgccg
     2461 cggtgtagag agattggacg ctcatggcag atttagaccc tcaggattcg gttaatcaat
     2521 tggctggttg cctggtcgtg ttgctgaatg agccggacgt tggcttcgtg ggcccgcgag
     2581 gcggcgatca gctcgaccat ttcctggacg ggcttgatcg ccgacagctc tcgaaagccg
     2641 ccgcgcactc ggcggtcgcc ggcctccacc ggacgcgagc tcttcgagga aaagtagttg
     2701 tgtcccacct tgaccaggtc gttcagcttg tccggccgga ccagggccaa ttccacgcgg
     2761 ctgccggcct ggtcgatgat gccgccgggc acgacgcgcc attcgcgcaa gggatcgatc
     2821 tgaatcgggt cgccgtcgga attcagcacc gagtcgccgc cgctggtttg cagcatccct
     2881 tcgttggaca actgaaagtt gccggcccgc gtgagcagcc gctcgctgcc gcgctggacc
     2941 aggaagaaca cgtcgggtac gtccccttcg atggcaaaat cggtcgggtt gtccgttttt
     3001 tgcaagtcgc cgggggcgaa gtcggtcgcc gtggcccgca gggctgcacc gccgccgacg
     3061 tcctcaactg tccgcgagcc gcgccgcgcc agcccctgct cgatggcctc gctctcgcgc
     3121 gactccagca gcgccagctc gcgcttgaag ccgacggtcg cgccgttggc gatgttgttg
     3181 ctgagcactt gcaccttctg gctctgcgag tgcgctccag cggccgacat gtagagcccg
     3241 tagtacatgg cggtcccgtt tagcaatcgc cgtgccgcgc gcggccgccg ccagctcgat
     3301 cgcacctgcc agcacgatta actggccgcg tcgcttggac ttagcgccaa atcgaccagc
     3361 gcactgtggt cgctgcctat cgcgccgatc catgctgcca gcggcaactg ctgccgatgc
     3421 cggccggcag atttcgccgt ggggcttatt ggccctggcg tgcgcccgca atcagttccg
     3481 cggcgccttg cgagagcagt tcttcggcga ccgcctggcc cagatagatg gcgtcgctca
     3541 cggtcatcgc cgcaccggcg gccaagcggt gcttgccatt agggctgagc acgacggctg
     3601 tgaggctcaa ttgctcaccg ctggccgtcg ccagagcgcc gaccggtgcg aggcagccgg
     3661 cgcggagtgt ggagagcagc gtccgctcgg ccaccacggc caggtgcgtc ggctcgtggt
     3721 cgagcggcgc gacgagcgac cgcgagcgaa cgtcgcccgc ccgcgtttcg attcccagcg
     3781 ccccctggcc gaccgcggag agcatcgtgg aacgcgggat cacctgccgg atgcgactcg
     3841 ccagccccag ccgcttcagg cctgcctcgg cgaggatgat cgcgtcgtag gctccctcat
     3901 cgagctttcg cagccgagta tcgaggttgc cgcgaatgtc ttcgatcacc aggtcgggcc
     3961 gcaaatagag gagctgcgcc ttgcggcgga ggctcccggt gccgacgcgg gcggcgtgcg
     4021 gcagcgtttc gatgctgccc gccttggccg aaaccaggac atccgccgtg ctttcgcgcg
     4081 gcggaaccgc cgcgatgatt agaccttcga ccggtgcggt tggcaggtcc ttcaaactat
     4141 ggacggcgag gtcgatttgc tcgtctagca gcgcccgctg caattccttg gtgaaaagcc
     4201 cctggccgcc gatctgaccc agcgggccgg atttcacgtc gccctgtgtg gtgatgggca
     4261 ccagttccac tggcgtgcca agctcggcca gccgggcggc gacccattcg gcttgccagc
     4321 gggccagcgg gctagcgcgg gttccgagcc ggagtttttc ctgggactgg gccatttttg
     4381 aagaatagcg ggctaccctt cggctgacga gggcagctgc cctcaggcgt cagaagtccg
     4441 gcgtgctgcg ggccaccgtg gcgacgtaga ccgcggcggc ccccgcctcg cgcaaggcgc
     4501 gcgacacttc ttgagccgtc gcgcctgtcg tcatgacatc gtccacgacc aggacgcgcc
     4561 ggccattcag ccgcgtccaa ggcgaggccc gaaacgcatc gcggacattg agctttcgct
     4621 gcggcggcgt gagcgaagct tgccgcgcca gcagacggcg gcaccgtatc agccgggact
     4681 gcagcggcaa accgagcgtt gtcgccacgg cgcgggcgag cgtttccgcc gtatgggtcc
     4741 gccgcacgag ccgcttcagc caatgcatgg gcaccggcgc caccaggtcg ggcatctcgg
     4801 cgagcggcct ggccagcagg caggcggcca ttcgctgacc gagcgccacg gtcagcggct
     4861 cgtacgcggc gtgcttgatc ttgagtacgg cctgccgcag tgcatcctga taggatccga
     4921 gcgtccgcac ggcggtgaag tgcagccgct ggccgcggca atatccgcag tcgccgcggg
     4981 gcagatcggt ctcggcacag atcaggccgc accgcgaaca gcgctggcgg ctggagacgg
     5041 cgaa
//
LOCUS       DASRJB010000732         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_777316, whole genome shotgun sequence.
ACCESSION   DASRJB010000732 DASRJB010000000
VERSION     DASRJB010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777316"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VFB80_19275"
     CDS             <1..1882
                     /locus_tag="VFB80_19275"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZN35984.1"
                     /translation="YEQWTGVVRFALDPNAAANQQIVDLKLAPRNAEGKVEFRADFRM
                     LVPADLSKANGAVFYEVNNRGGATAPRLIEASADDFLCRQGFVVLWSGWIAQVQPGDG
                     RLRLQAPIATEAGMPIRGIVREEVIVDRPQPKAAISHRGNQGSYLPTERGFASAKVTR
                     REREADPRQEVPRDEWKLVSSMVGTPQESGQLPLIELEIAGGLKPGWIYEVVYEAEGP
                     IVQGVGLAGIRDILSALKFGNVDQNPLRTADGKPVVSRALGFGTSQSGRLLRHFLWDG
                     FNADEQGRKAFDGVISHVAGGGLGYFNHRFASPTRTNGQHDEHLFPADYFPFTYGDMS
                     DPLSGKTDGILAKSRRTNTTPKVFHTQSSSEYWHRSGSLVHTDPLAKTDAEIPPDVRI
                     YTFGGTQHGSGSGFPGPRGNGQLPASPADYSPLMRALVVALDAWVKDGAEPPPSAYPR
                     IADGTLADWQRDKARWPAIDGVNYPAVIQQPHFLDRGPKWDSQRISAIEPPAVKGNYV
                     VKVPAYDADGNEKGTLNLPAITVPVATYTSWNLRHESIGAAGELLSLQGGYIPFAKDP
                     ASRAAAKDPRPSIAERYKSYGDYEQRYMEAAEKLLAQRYLLAEDLPRLKALCERFKPV
                     FE"
     gene            complement(1963..3117)
                     /locus_tag="VFB80_19280"
     CDS             complement(1963..3117)
                     /locus_tag="VFB80_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35985.1"
                     /translation="MVAEWYRTRDYNFLALTDHNVLSQGMRWIRESDVTRRGGQTVLP
                     KYLARFGPDWVERRGADALAEIRLKPLDEFRALVEERGKFIMIPAEEISDSSEGVPVH
                     LNASNLRDLVEPAKGATVRETIENNLRAVEAQAKSAGREILAHLNHPNFGWAVTAEDI
                     AHAVTDRYFEVYNGHPGVNQPGDDHRPGIDRMWDIANTIRLVELKAPPLYGLATDDSH
                     AYHNKPNAARPGRGWQMVRATHLTPETIIRAIKAGDSYASSGVTLSDVRYDREQKTLS
                     LTIQPTAGATYVTQFIGTLRGTDTASQPTLTAAGSPPTDKNGKPLRVSRKYSSEIGQV
                     LRAVTGLSASYKLTGKELYVRAVVTSSADPADPSFAGQKQQAWTQPVGWE"
BASE COUNT          525 a         1053 c         1127 g          547 t
ORIGIN      
        1 gtacgagcag tggaccggcg ttgtgcgttt cgctctcgat ccgaatgccg cggcgaacca
       61 gcagattgtc gatctgaagc tcgccccgcg gaacgccgag ggcaaggtgg agttccgggc
      121 cgatttccgg atgctcgtgc ccgccgatct gagcaaagcc aacggcgcgg tcttctatga
      181 agtcaacaac cgcggcggag caaccgcccc gcgattgatc gaggctagcg ccgacgactt
      241 cctctgccgg cagggctttg tcgtgctctg gagcggctgg atcgcccagg tgcagccggg
      301 cgacggacgg ctgcgcctgc aagcgccgat agcgaccgaa gcgggcatgc cgatccgcgg
      361 gatcgtgcgg gaagaagtga tcgtcgatcg gccgcagccg aaggctgcga tttcgcaccg
      421 cggcaatcag ggcagctatc tgccgactga gcgtggcttc gcctcggcca aggtcacccg
      481 ccgcgaacgc gaggccgatc cgcggcagga agtgccgcgg gatgaatgga agctggtcag
      541 cagcatggtc ggcacgccgc aggaatcggg ccagctgccg ctgatcgagc tggagatcgc
      601 tggcggcctc aagcccggct ggatttacga ggtcgtctat gaggcggaag ggccgatcgt
      661 gcagggcgtc ggcctggccg gcattcgcga cattctctcg gcgctgaagt tcggcaacgt
      721 ggaccagaac ccgctccgca cggcggacgg caagcccgtc gtttcgcggg cgctcggttt
      781 cggcacttcg cagagcggcc gcctgctgcg gcactttctg tgggacggtt tcaacgccga
      841 cgagcagggc cgcaaggcct tcgacggcgt gatctcgcac gtggccggcg gcggcctggg
      901 ctatttcaat caccgcttcg cctcgccgac gcggaccaac ggccagcacg acgagcatct
      961 cttccccgcc gattacttcc ccttcaccta cggcgacatg agcgatccgc tcagcggcaa
     1021 gaccgacggc attctggcca agtcgcggcg gacgaacacg acgccgaagg tgtttcacac
     1081 gcagtcgtcg agcgaatact ggcaccgcag cggctcgctg gtgcacaccg atccgctggc
     1141 caagaccgac gccgaaattc cgccggacgt ccgcatctac acgtttggcg gcacgcagca
     1201 cggcagcggc agcggctttc ccggtccgcg agggaacgga caactgcccg ccagcccggc
     1261 cgactattcg ccgctgatgc gggcgctggt cgtggccctc gatgcctggg tgaaggatgg
     1321 cgccgagccc ccgcccagcg cctatccgcg gattgcggac ggcacgctcg ccgactggca
     1381 gcgcgacaag gccaggtggc cggccatcga cggcgtcaat tatccggcgg tgatccagca
     1441 gccgcacttt ctcgaccgcg ggccgaagtg ggatagccag cggatcagtg ccatcgagcc
     1501 gccggccgtg aaaggcaact acgtggtgaa agtcccggcc tacgacgccg acggcaacga
     1561 gaagggcacg ctcaacctgc cggccatcac ggtgcccgtc gcgacctata cgagctggaa
     1621 cctgcggcac gaatcgatcg gcgcggcggg tgagttgctg tcgctgcagg gcggctatat
     1681 accgtttgcg aaggaccccg cgagccgagc cgccgccaag gacccgcggc cgagcatcgc
     1741 cgagcgctac aagagttatg gcgattacga gcagcgatac atggaagccg cggagaagct
     1801 gctggcccag cggtatttgc tggccgagga tttgccgcgg ctgaaggcgc tctgcgagcg
     1861 gttcaagccg gtatttgagt aggtcccgcc tgccgggcgg gaccttgggg cggataaacg
     1921 tcgctgcgct tccctgaggc cccttccggc agaagaggcc tactactccc aacccaccgg
     1981 ctgcgtccag gcttgctgct tctgcccggc gaacgacgga tcggccgggt cggcgctgga
     2041 ggtgacgacc gcccgcacgt acagctcctt gccggtcaac ttgtagctcg cggaaaggcc
     2101 ggtcaccgcc ctaagcacct ggccgatctc gctcgaatac ttgcggctga cgcggagcgg
     2161 cttgccgttc ttatcggtgg gcggcgagcc ggccgcggtc agcgtgggct ggctggcggt
     2221 atctgtgccg cggagcgtgc cgatgaactg cgtgacgtag gtcgcgccgg ccgtgggctg
     2281 aatcgtcagg gacagcgtct tctgctcgcg gtcgtagcgc acgtccgaga gcgtcacgcc
     2341 gctcgacgcg taactgtcgc ccgccttgat cgcccggatg atggtttcgg gcgtgaggtg
     2401 cgtcgcgcgg accatctgcc agccgcggcc cggccgggcg gcgttgggct tgttgtgata
     2461 ggcgtggctg tcgtcggtgg cgaggccata gaggggcggc gctttcagtt cgaccaggcg
     2521 gatcgtgttg gcgatatccc acatccggtc gatcccgggc cggtggtcgt cgcccggctg
     2581 attgacgccc ggatggccgt tgtagacctc gaagtaccgg tcggtgacgg cgtgggcgat
     2641 gtcctcggcc gtgacggccc agccgaagtt cgggtgattg aggtgggcga ggatttcgcg
     2701 gccggcggac ttggcctggg cctcgacggc gcggaggttg ttttcaatcg tctcgcgaac
     2761 ggtcgcgccc ttggcgggct cgaccaaatc ccgcaagttg ctggcattga ggtgcaccgg
     2821 cacgccttcg ctgctgtcgc tgatctcctc ggcgggaatc atgatgaact tgccccgctc
     2881 ttcgacgagg gcccggaact cgtccagcgg cttaaggcga atctcggcca gtgcgtccgc
     2941 tccgcgtcgc tcgacccagt cgggaccaaa acgcgcgaga tacttgggca gcacggtctg
     3001 tccgccgcgg cgcgtcacat ctgactcgcg aatccaccgc atcccttgac tgagcacgtt
     3061 gtggtcggtc agggccagga agttgtagtc ccgcgtgcgg taccactccg cgaccatctc
     3121 gggaaagtcg tcgccgtcgc tccagaaggt gtgcgtgtgg aggttgcctt tccatcaccg
     3181 cggctgcggc cgggcgggaa ccgcatcgtc ctgggcctga atcgccgcgg cgactgccgc
     3241 gagccagagg aa
//
LOCUS       DASRJB010000733         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_777570, whole genome shotgun sequence.
ACCESSION   DASRJB010000733 DASRJB010000000
VERSION     DASRJB010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777570"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFB80_19285"
     CDS             complement(<1..958)
                     /locus_tag="VFB80_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN35986.1"
                     /translation="MPSRCRRPAFTLVELLVVIAIIGVLVALLLPAVQSAREAARRMS
                     CSNNLKQLGLALHNYHDTFQRFPPPGLNYGWCRNPPPATGASNNRILNVNGLALMMAF
                     YEQGAAAGQYVNTAAASNVTVGNESCCGPTVAQEPLAGGGVPTTNAEIVRLRLKIFLC
                     PSDMTDPSIPNTTDIYSIGYNRGNLGRGARTNYDFSSLLSYQCDEWTRQRNNNTRRMF
                     AQNSDCRFADVTDGTSNTVAYCESTRDTDNGETTTWGYRGWVMIGNDLGTSAGINNWI
                     YNNDPARRKVGRVGSWGWPGSLHPNGCMVGMGDGAVRFVPQNA"
     gene            complement(1394..1843)
                     /locus_tag="VFB80_19290"
     CDS             complement(1394..1843)
                     /locus_tag="VFB80_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35987.1"
                     /translation="MPAVYENLGVRLLYPENWSITDEEDDGWPRSVTIQSQDTGFWSL
                     HVYPAGQKLKPVVDELVAAISDDFGEVEVLPAEETFGDTVTTGVDLAFFYLDLLVEAK
                     IRCVRTPSCTLVWLYQAESREFEAQEPVFQAMAVSMLQTQVAVKSGR"
     gene            2038..2787
                     /locus_tag="VFB80_19295"
     CDS             2038..2787
                     /locus_tag="VFB80_19295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN35988.1"
                     /translation="MQIPPGFAAALPSIVAAVEAVPGYLTRREIEFLVLLGACPTAEG
                     AIVELGTLYGKSAVALAKGAALAGGDAVFSVDIQERLEARHFLARNRVADAIELRVQS
                     SAEFWPQFDQPIRLFWHDGANSKAIVECDIRAALMRLSDGAIIAFHDVLNPSGERLHP
                     FVDSILSDQRFGAAGVVGSIGWARFRRAGCSIDEADAKKRLAWRLSNLKPFHGVPARK
                     PRGLRKVVYEVCRQLVPHGAIRPEQFLRKVA"
     gene            2929..3861
                     /locus_tag="VFB80_19300"
     CDS             2929..3861
                     /locus_tag="VFB80_19300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN35989.1"
                     /translation="MPASSTRIALWLLLAGSPLAASLGCRAFRCQEVSDEAIAATRQL
                     SLQGMDAQQRGRWDQAESLFAQAVITCPRDERARCGYAESLWHRGAREQAVTHMEEAV
                     RLSGHDPERLVQLGHMYREQGDLSRAGNLAARAIHANPQLASAWALRGEVERASGQRT
                     EALASFHRALSLQPHFPPVQLAVAEIYAAENRPQQALATLQALEASFPPGQGPVDLMV
                     RQSRALCSLGRYPDALRKLTTAAERVNPSADLLCELAQTQLLAGETSAAQLTLSAALA
                     RDPRHPGCLALEQRLGAPQGALATALAPQQTVLH"
     gene            complement(3894..4115)
                     /locus_tag="VFB80_19305"
     CDS             complement(3894..4115)
                     /locus_tag="VFB80_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35990.1"
                     /translation="MSENACPEAASMASCPGGMARQFRVQVWNGETPSHWKLVGSFAD
                     LRDACRCAEQLQDAGEQIRVVACRLPTAA"
     gene            complement(4561..4920)
                     /locus_tag="VFB80_19310"
     CDS             complement(4561..4920)
                     /locus_tag="VFB80_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35991.1"
                     /translation="MRMFRFTIRELVLLVALVAMGLGWWVHVRRLESQRRSLERDLLM
                     QADFVQQFVSVLTKLGCEIPPDSDPRCAVGGPYTRIFVPKEMQVLTHCVSFTLSGPRS
                     ELQRTIDGEFQRFRKPQ"
BASE COUNT          928 a         1785 c         1635 g          925 t
ORIGIN      
        1 cggcgttctg cggcacgaac cgcaccgcgc cgtcccccat gccaaccatg caaccgttgg
       61 gatgcaggct gccgggccag ccccagcttc cgacgcggcc gactttgcgc cgcgccgggt
      121 cgttgttgta aatccagttg ttgatgcccg ccgacgtccc cagatcgttg ccaatcatca
      181 cccagccgcg atagccccaa gtcgtcgttt cgccgttgtc ggtgtcacgg gtcgattcgc
      241 aataggcgac cgtgttgctc gtgccgtcgg tcacgtcggc aaagcggcag tcgctgttct
      301 gggcgaacat ccgccgcgtg ttgttgttcc gctgccgcgt ccactcgtcg cactgatacg
      361 aaagcagcga ggagaagtcg tagttcgtcc gcgcgcctcg gccgaggttc ccccggttgt
      421 agccgatgga atagatgtcc gtggtgttcg ggatggatgg atcggtcata tcgctggggc
      481 agagaaagat cttcagccgc aagcggacga tctcagcgtt ggtcgtcggc acgcctccgc
      541 cggccagagg ctcttgggcc accgtcggac cgcagcagct ttcattgcct acggtgacgt
      601 tgctggccgc cgccgtattg acatactgcc ccgctgcagc accttgttca tagaacgcca
      661 tcatcagcgc caggccgttg acattcagga tgcgattgtt cgaggcacct gtggccggcg
      721 gcggattgcg acaccagccg tagttcaggc ccggcggcgg aaaccgctga aaggtgtcgt
      781 gatagttgtg cagggccagg ccgagttgct tgaggttgtt cgagcagctc atgcggcggg
      841 ccgcttcgcg ggccgattgc accgccggca acagcagcgc gacgagcacg ccgatgatcg
      901 caatcacgac gagcagttcg accagcgtga aggccggccg ccggcaacga cttggcatgg
      961 agttcactcc ggtcggattg ggaagacctg tctcatttta agaacggacg gaatcgcaac
     1021 acaagcagcg cggcctatcg ggtcagggcg gaacggccgc cgtaactagc tgcggccaaa
     1081 taggccatta attgccccgt gtcgaaaagt ctgcgtcgcc cgattcgggc gtcgattggc
     1141 cgcaagtatc ggctggcaac ggagttgtat taaacacaca gtcactatag tcacattagg
     1201 gtttgaaagc tttgaagagt tagaggggag ttttcgttat attcttgccc gccgcgctgc
     1261 ggcggcgact tctccctaac gctcaccggc ctcgggcaac caccggccgc gccgtcacac
     1321 caaaatggca gtccgctgcg caattggaac tatactgcaa ttagcgtacc aatgtttacg
     1381 cacgggcggt cggtcagcgg cccgacttta ccgccacctg cgtctgcagc atgctcacgg
     1441 ccatcgcctg aaacaccggc tcctgggctt cgaactcgcg gctctcggcc tggtacagcc
     1501 agacgagcgt gcaggacggc gtgcggacgc agcggatctt ggcctcgacc agcaagtcga
     1561 gatagaagaa cgccagatcg acgccggtgg tgaccgtgtc gccaaaggtt tcctcggcgg
     1621 gcagcacttc cacctcgccg aagtcgtcgc tgatcgccgc gaccaactcg tccacgacgg
     1681 gcttcagctt ctggccggcc ggatagacgt gcagcgacca gaaaccggtg tcctggcttt
     1741 gaatcgtcac gctccgcggc cagccgtcgt cttcctcatc ggtgatcgac cagttctccg
     1801 ggtagagcag gcggacgccg aggttctcgt agacggctgg catggcggcc tccaccgcgc
     1861 gggaattcct tgaacctaac acgcgcccac tgccgcgaat agtaacggct gccgctagca
     1921 agctggcgct gcgatgaatc gagtcgggca ggcggagagt ttgtctcacc atagctcccg
     1981 gcgaagtccg ataacgttgc caccgctgcg cccctatttt ttcccgaggg ccgaccgatg
     2041 cagatcccac ctggattcgc cgccgccctg ccgtccatcg tcgccgccgt cgaggccgtg
     2101 cccggctatc tgacgcggcg cgagatcgag ttcctcgtgc tgctgggggc ttgccccacc
     2161 gccgaaggcg cgatcgttga gctgggaacg ctgtacggca agtcggcggt ggcattggcc
     2221 aaaggcgcgg ccctggcagg cggcgatgct gtcttctcgg tcgatatcca ggaacgcctc
     2281 gaagcccggc acttcctggc ccgcaaccgc gtggccgacg cgatcgaatt gcgagtgcag
     2341 tcgtccgccg aattctggcc gcagttcgac cagccgatcc gcctgttctg gcacgacggg
     2401 gcaaactcca aggccatcgt ggaatgcgac atccgcgccg ccctgatgcg gctgtccgac
     2461 ggcgcgataa tcgcctttca cgacgtgctg aacccgtccg gcgaacgctt gcaccccttc
     2521 gtcgattcga tcctgtccga ccagcggttc ggggcggccg gcgtcgtcgg ctcgatcggc
     2581 tgggcccgct ttcgccgggc cggatgctcg atcgacgagg ccgacgcaaa gaagcggctc
     2641 gcctggcggc tgtcgaatct caagcccttc cacggcgtgc cggccagaaa gccgcggggc
     2701 ctgcggaagg tcgtgtacga agtctgccgg cagctggtgc cgcacggcgc gatccgcccc
     2761 gagcagttcc tccgcaaagt tgcctagccc aaggttgcct agcccaaagg ttgcctagcc
     2821 acaaggatgc ctagccacaa ggttgcctag ccgcgcggcg ttgttcgcct cgcgcgaatc
     2881 tggtatctgc cgcaggtgca gcgattccat ccatgggagc agggccggat gccagctagc
     2941 agcacccgca tcgccctctg gctgctgctc gccggctcgc cgctagcggc cagcctcggc
     3001 tgccgggcgt ttcgctgcca ggaggtctcg gacgaggcca tcgcggccac gcggcagctg
     3061 tcgctgcaag ggatggatgc tcagcagcgc ggccgctggg accaggccga gtcgctcttc
     3121 gcccaggccg tgatcacctg cccgcgcgac gaacgggccc gctgcggata tgccgagtcc
     3181 ctctggcacc gcggagcccg cgagcaggcc gtcacgcaca tggaagaggc cgtccgcctc
     3241 tccggccacg atcccgagcg gctggtgcag ctcggccaca tgtaccgcga gcagggcgat
     3301 ctgtcgcggg ccgggaacct cgcggcccgc gccatccatg ccaatccgca actcgcctcg
     3361 gcgtgggcct tgcggggtga agtcgagcgg gccagcgggc aacgaaccga agcgctggcg
     3421 agttttcatc gggcccttag tttgcagccg cactttccgc cggtgcagct ggcggtggcg
     3481 gaaatctacg cggcggaaaa ccgtccgcag caggcgctgg cgacgctgca ggcgctggag
     3541 gccagctttc cgccgggcca agggccagtg gatctgatgg tccgccagag ccgggccctg
     3601 tgcagcctcg gccgctatcc cgacgcgctg cgcaagctca ccaccgccgc cgaacgcgtg
     3661 aacccatcgg ccgacctgct ttgcgagctg gcccagacgc agctcttggc cggcgagact
     3721 tccgccgcgc agctcacgct atccgccgcc ctggcccgcg atccgcggca tcccggctgc
     3781 ctggctctcg agcagcggct cggcgcgccg caaggcgctc tcgctactgc gcttgcgccg
     3841 cagcaaaccg tgctgcatta atgaccaatg accttaattt ggtgcttccg ccgttaggcg
     3901 gcggtcggca ggcggcaggc gacgacgcgg atttgctcgc cggcgtcttg cagctgctcg
     3961 gcacagcggc aggcgtcgcg caggtcggcg aagctgccga cgagcttcca gtgcgagggc
     4021 gtttcgccgt tccaaacctg gacgcggaat tgccgcgcca ttccgccggg gcagctcgcc
     4081 atgctggcgg cttccggaca tgcattttcc gacattcaat cgctcctctg ccggcggcgc
     4141 gacaggcaat agtagccagc agccaatacc tgatatcggc cggcaccggt agcccgaatg
     4201 aaggccgcta tcatcgctgg cgcagcacgc cgcgttttgc taccacaaaa agcccggcct
     4261 ttattgttct gccggccttg ccagacacac cggcccgtct gcggttcgct ggcgtacacc
     4321 aagcgcgacg cgccagcgag gttcgggagg gcgaggctcc cgccgggccg atttcggcaa
     4381 agccgcgacc attggtcgcg gtgcccgccg gcagccattg accgcgacca atggtcgcgg
     4441 ctttgaggca caccgcagat tccgccgcaa cccatgccag ggaaataact tgcgccacaa
     4501 atccgccgcg catgattatg cgccttatgc gcgccggccc attccgcccg gctacgacct
     4561 tcactgcggt tttcggaacc gttggaactc gccgtcgatg gttctctgca gctcggaccg
     4621 cggaccagag agcgtgaacg agacgcagtg ggtcagtacc tgcatttcct tggggacgaa
     4681 gattcgggtg taaggaccgc cgaccgcgca gcggggatcg ctatccggcg gaatctcgca
     4741 gcccagcttg gtcagcaccg agacaaactg ctggacaaaa tcggcctgca tcagcaaatc
     4801 gcgctccagg ctcctccgct ggctctccag ccgccggaca tgcacccacc accccaggcc
     4861 cattgcgacc agagccacca gcagcaccaa ctcgcgaatc gtgaaccgaa acattcgcat
     4921 acaaaacccc cgccaatcag acgtgcccag aggccaacca gttcttgctc ccaaagccgc
     4981 gaccagtggt cgggctgctc ggggcccgtg cggagtggta gggcgcgacc ctggtcgcgg
     5041 ctttgaacct cagcccatgc gcgcatagcc cgcagaattc tccgccagcc cagaatccgc
     5101 cgcaactctc gctctctcaa tgacttgcgt ccaaaacccg ccacgcatga ttatgcgcat
     5161 tatgcgcgcc ggcccattcc gaattcctct cccgccggga gaccacaacc aaattgagac
     5221 atagcgaaac ctgccaaatt caagcgaatt cattctatgt aaacacctga tca
//
LOCUS       DASRJB010000734         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1337468, whole genome shotgun sequence.
ACCESSION   DASRJB010000734 DASRJB010000000
VERSION     DASRJB010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337468"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VFB80_19315"
     CDS             complement(<1..1882)
                     /locus_tag="VFB80_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HZN35992.1"
                     /translation="MNSPNLSANEGVFRAEAYSAEHLKHHARQLASIHTLQERPASDR
                     RFVARFAANAEFLRKTYDEVAAAVRQGEPVTAEAEWLLDNYYIVEEQLREIRDDLPQR
                     YYRELPKLASGEPRVYALALELVLHTDSALDEETLVRFLREYQTAAPLSIGEVWAVPI
                     MLRLVLVENLRRVAAQMVATRRCRQEARRIWEAWQPHEPFPLDLSSLDSCAAVVLHLV
                     EHIHERGAAAGPALKRLEQQLADAALSAHDVTHHEHRRQAANQVSIGNIITSMRLISS
                     LDWVQFFERTNLAEQILRLDPAEVYPQMDFETRNRYRDAVEQLAKRTKFSDLEIAQRA
                     IARCQPAPGDAPARWQERHVGYWLIDRGREQFEQEIDYRPPSVQRWRRWLIAHPAATY
                     FGSILAWTALLLGMLLSLASAAGLSVAALAAVALLLVMPAVDLAVSLTNLLVTVWLPP
                     RLLPKLEFKEGVPATFATFVVVPSLLTSRREVQSLLQRLEMHYLANPDQALRFALLAD
                     FADAPERTLPGDKNLVDQALEGIRQLNTRYGDARRQPFYLFHRERRWNEQEGCWMGWE
                     RKRGKLMEFNRLLAGAADTTYAVQEGDLASLKPTGQHGSVQFVMTLDADTELPFGAAR
                     KLI"
     gene            complement(1920..2087)
                     /locus_tag="VFB80_19320"
     CDS             complement(1920..2087)
                     /locus_tag="VFB80_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35993.1"
                     /translation="MPTLAELRNRTSVVRTSVNVSKPKEEALVADRRSGLRPAHIVEQ
                     KLTVALSKSKS"
     gene            2418..>2924
                     /locus_tag="VFB80_19325"
     CDS             2418..>2924
                     /locus_tag="VFB80_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN35994.1"
                     /translation="MFRTLSTTAGSFALGIAVLLSQAGCEKESPPGGPAATNDTTSNA
                     TNTNPSGTTDTAKDGFKLSVPSTRTVIEPGARTEVAIKISRDGNFKEPVELTFKAPEG
                     VTAQAAKPQFEPNDAEVMLTLEAAADAKPGEASIEVTGTPQTGSPTTVAVPIEVRART
                     NSAKPAAPD"
BASE COUNT          552 a          943 c          944 g          485 t
ORIGIN      
        1 cgatcagctt gcgggccgcg ccgaacggca actcggtgtc ggcgtcgagc gtcatgacga
       61 attgcacgga gccgtgctgt ccggtcggct tcaggctggc gaggtccccc tcctgcacgg
      121 catacgtggt gtcggcggcg ccggcgagca gccggttgaa ttccatcaac ttgccgcgct
      181 tccgctccca acccatccag cagccttcct gctcgttcca gcgccgctcc cgatggaata
      241 gatagaacgg ctgccgccgc gcgtcgccgt aacgcgtgtt caactgtcgg atgccctcga
      301 gggcctgatc gacgaggttc ttgtcgccgg gcaaagttcg ctccggggca tcggcaaagt
      361 cggcgagcag cgcaaatcgc agcgcctggt cgggattagc cagatagtgc atttccagtc
      421 gctgaagcag cgactgcact tcgcggcgcg aggtcagcag ggatggcact acgacgaacg
      481 tggcgaacgt cgcgggtact ccctctttga attcgagctt cggcaacagc cgcggcggca
      541 gccagacggt caccagtagg ttggtgaggc ttacggccag gtccaccgcg ggcatgacga
      601 gcagcagcgc cactgctgca agtgctgcca cagataatcc cgcggcgctc gccagcgaca
      661 ggagcattcc cagcaggagg gcagtccagg cgaggatgct gccgaagtaa gtggcagcgg
      721 gatgggcgat cagccaccgc ctccagcgct gcaccgacgg cggccgataa tcgatttcct
      781 gctcgaactg ctcgcggccg cggtcgatga gccaatagcc gacgtgccgc tcttgccacc
      841 gcgcgggtgc atcgccgggc gctggctggc agcgggcaat ggcccgctgg gcaatctcca
      901 agtcgctgaa cttcgtccgc ttggccaact gctcgacggc atcccggtag cggttgcggg
      961 tctcgaaatc catctgcgga tagacctcag ccggatccaa ccgcaggatc tgctcggcca
     1021 gattcgtccg ctcgaagaac tgcacccagt cgagcgacga gatcagccgc atgctggtga
     1081 tgatgtttcc gattgaaacc tggttggcgg cctggcggcg gtgctcgtgg tgcgtaacgt
     1141 cgtgggcgga gagcgccgcg tcggccagct gctgctcgag ccgcttgagc gccgggcccg
     1201 cggcagcccc ccgctcgtga atgtgctcca ccaggtgcag cacgacagcc gcacagctgt
     1261 cgagcgacga caggtcgagc ggaaacggct cgtgcggctg ccaggcttcc cagattcggc
     1321 gggcctcctg ccggcagcga cgggtggcga ccatctgcgc ggcgacgcgc cgcaggtttt
     1381 cgacgagtac gagccgcagc atgatcggaa cggcccagac ctcgccgatc gagaggggcg
     1441 ctgcggtctg gtattcgcgc aagaagcgga cgagcgtctc ttcgtcgagc gcactgtcgg
     1501 tgtgcagcac cagttccagg gccagggcat agacccgcgg ctcgccggac gccagcttgg
     1561 gaagctcccg gtaataccgc tgcggcaggt cgtcgcgaat ctcgcggagc tgctcctcga
     1621 cgatgtaata gttgtcgagc agccactcgg cttcggccgt tacgggctcg ccttgccgca
     1681 cggcggccgc cacttcgtcg taggtcttgc ggagaaactc ggcattcgcg gcaaaccggg
     1741 cgacgaacct ccggtcggac gcggggcgct cctggagcgt gtggatggaa gctagctgcc
     1801 gcgcgtgatg cttgagatgt tctgcgctat aagcttcggc gcgaaaaacg ccttcattcg
     1861 cagaaagatt cggcgagttc accgatcagc acagctgcgc gagcacagcc gcaaggaggt
     1921 caggacttgc tcttggacaa ggcgacggtc agcttttgct ccacgatgtg cgcggggcgc
     1981 agcccgctgc ggcggtcggc gaccagggcc tcctctttcg gtttactcac gttgaccgaa
     2041 gtccgcacca cgctcgtacg gttccgtaat tcggcaaggg tcggcatgac ggctccttcc
     2101 aaaaagagat ttcgagagcg agcgcagaac tctcacccaa ctgaagcaag acgcgtgccg
     2161 gtgctaagcc caaatgccgg actcgtgagc acgccgccgg tttgccgaat tccttgcaat
     2221 ttgcggcgaa tccgatagtc cagcgaaccc gctccggtgg agcagcgccc gcgcgcggag
     2281 gaaccgcgac attttgtcag gcaatcacgt acgaatcgcc gtcagtgtgg tcgccaataa
     2341 agcaagcgag cgcgggcatg cgaaatgcaa ttctgttgat gcgttgtccc ttcaacttgc
     2401 atttcatgag gtcaatcatg ttccgcacac tttcaaccac cgcaggctcg ttcgcattgg
     2461 gaatcgctgt gctgctgtcg caggccggct gcgagaagga aagccctccc ggcgggccag
     2521 ccgccaccaa cgacaccaca tcgaacgcga cgaacacaaa tcccagcggg acgaccgaca
     2581 cagccaagga cggcttcaag ctctctgtac cctcgacgcg cacggtgatc gaaccgggag
     2641 cgcgcacgga ggtcgccatc aagatcagcc gcgacggcaa cttcaaggaa cccgtggagc
     2701 tgacattcaa ggcgccggaa ggcgtgactg ctcaggccgc caagccgcag tttgagccaa
     2761 acgacgccga ggtgatgctc acgctggaag cagcggccga cgccaagccg ggcgaggcca
     2821 gcatcgaagt cacgggcacg ccgcaaaccg gcagcccgac gaccgttgcg gtgcccattg
     2881 aagtccgtgc ccgcacgaac agcgctaagc ccgccgctcc ggac
//
LOCUS       DASRJB010000735        12940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_777761, whole genome shotgun sequence.
ACCESSION   DASRJB010000735 DASRJB010000000
VERSION     DASRJB010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777761"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFB80_19330"
     CDS             <1..355
                     /locus_tag="VFB80_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN35995.1"
                     /translation="FTAFHDYFKGDLKGWTSDRYMPKIRDAYENNPDKMPFDFPEVLA
                     AIAPRGVYVAAPLHDSNFENSGVRKCIASAQPVFELLGAKDQIVAEYPDCGHDFPDDV
                     RERVYSWLEKQLRKE"
     gene            495..1802
                     /locus_tag="VFB80_19335"
     CDS             495..1802
                     /locus_tag="VFB80_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZN35996.1"
                     /translation="MPWFTESASSISRSRVEQLVQRALDEAKSRICKTPRRVLLLPPD
                     ITRMHSGSGWITEAFYRLLKDSADVHVIPTLGQHEPHTREQNAQMFGSIPHERIHAHD
                     WRGGCTHLGDVPADYVKEASGGAADWKFPVWLNSMLMQEPWDLIINIGHVVPHEVLGF
                     ANHNKNYFIGLGGKDLICAAHMAAACCGIENNLGNLLTPVRQCFNWAEEKFLARLPDF
                     YVQVVLARDEQGRLAHSGVFVGDDLETYLAAARQAREQNITTFDEPLQKVVCVMQGDE
                     FFSTWVANKAVYRTRMALATGGELLIIAPGLKRFGEQADVDQLIRKYGYCGTPRVMEL
                     YQQNADMQDLAHASAHLIHGSSEGRFRITYAPGHLARADIEQVKFGYADLAATLRRYP
                     VERLREGFNDIDGERIFFIPTPSAGLWATKAKLYGRKSGFGLD"
     gene            2046..3794
                     /locus_tag="VFB80_19340"
     CDS             2046..3794
                     /locus_tag="VFB80_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZN35997.1"
                     /translation="MRTTCAAQGKSARRGNIVVFSAFLMAIFMAMLALSVDLGFIFTM
                     KAQLQRAVDAAALAAAGELVNGIPAAEAAAREYLVRNPVGSAQGSIGGDLGEALVTFE
                     EQHADDYTFTAGNWNTTTRTLEETSVMPAAMKVSMTYPNLPLFFARVMGHDHFSLTAE
                     STAMFHPRDIVVVLDFSASMNDDSTFNAIGKLPQSQIEGSLLECWSDLGPPVYGNLPA
                     TPTWATAHGVPANIATGLPHCTVRYQNTAVYVTSTLNLATVKLEFSNGAQQTFTPGAV
                     TNGTFTGTGGNANKQVRKVWVKSGTNVSLGLNGEFFDFTSANINTTLKKALGLNTVTY
                     PYPNGGNWDGYINYCSNSSNGNKNAGFRYKFGGMNLVEYWLTSFPGYNQVPDLWKTRA
                     EPVYALKDANGVFMDFIGQVNTLDRVALVIYNAADGNAIMESNFTHDLDSITNIVNHR
                     QAGHYHQYTNIGAGLQKGRMVMDSYARQNANKLIVLMTDGLANWHNGQYDLAGAAQMI
                     EEEANLCAADSRKYKVTTVSVGVEADTETMADVANVTGGKFYVVPGGSTHEAMHAQLK
                     TAFEEIAKARPIKLVQ"
     gene            4259..5548
                     /locus_tag="VFB80_19345"
     CDS             4259..5548
                     /locus_tag="VFB80_19345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN35998.1"
                     /translation="MPMRRRVVVTGMGCVNPLGHDVESMWSALRESQSGVGYTTIFDA
                     SKFPTQISAEVKNWDISQAGEDPQAWKYRARHTRFACGSAKQAMRQAGLEGGPPDPTR
                     MGVYLGCGEGNQDFLAFSKMMTAALRPDGLDLATFARTGLEILNPVAEIEQEPNMPAG
                     YIAGLFNAQGPNSNCLTACAASSQAIGEATEIIRRGDADLMLSGGAHSMIHPFGVTGF
                     NLLTALATKYNDQPTKASRPFDRLRDGFVLGEGGAMIVLEELEHARRRGAPILGEIMG
                     YGTTADAYRITDIHPEGRGAIACMKMAIADAELNASDIQYVNAHGTSTAVNDRVETVA
                     CKQVFGERALQTPVSSTKSMMGHLIAAAGVTEAIVCLLAIRDNVLPPTINYENPDPDC
                     DLDYVPNQAREQRCDYALSNSFGFGGQNISLVVGRFS"
     gene            complement(5615..6805)
                     /gene="truD"
                     /locus_tag="VFB80_19350"
     CDS             complement(5615..6805)
                     /gene="truD"
                     /locus_tag="VFB80_19350"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZN35999.1"
                     /translation="MKLKRLAEDFQVEEQVSLAPSGGPLAMYLLTKESLGTLEAIEAI
                     RRRWNLDRGRIAFAGLKDKHAQTRQFVTIEGGPPRNLSEESFRLEYLGPAARPIHASD
                     ILSNAFTVVIRELSEAEAQAASAALSAATQFGVPNYFDDQRFGSLGESGHFIAEPWCR
                     GDYERTLWLALAEPNARDRPEDREQKRLLRDHWGQWQRCGELLARSERQRVAGFLESS
                     RGDFRRAIGLLRQDLRSLWLAAYQSQLWNQVLAALLREVCRPEQLAPQTIGRRELPLI
                     AALESEQFERLRATQLPLPSARLHLERGPLQELYDRVLAAEGIALRELRVKYPRDSFF
                     SKGERAVLLLPQAVEQSAAADELYPGRRKLTLRFSLARGSYATILIKRVTGSMVEWEG
                     DDVV"
     gene            complement(6802..7365)
                     /locus_tag="VFB80_19355"
     CDS             complement(6802..7365)
                     /locus_tag="VFB80_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36000.1"
                     /translation="MPRWHSILLIVSGGLLAWLLMQAVHELGHVAAAWWTGGKVVQVV
                     LHPLAISRTDVDPNPQPLIVAWSGPLVGSVLPLAIWGLAALLRLRLAWLARWFAGFCL
                     LANGLYIGVGSFGGIGDAGDMLRSGAPPWTLWLFGTIGALAGLALWNGQGRHFGLGSA
                     AQPVDPRVAYGCALALAATAVAEILAS"
     gene            complement(7369..7734)
                     /locus_tag="VFB80_19360"
     CDS             complement(7369..7734)
                     /locus_tag="VFB80_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36001.1"
                     /translation="MAKCEEGYLCSVCGGDVEQIYESDLYLRYVIGQVDPETLHTTRE
                     RHIRCNPTLAQFIVDPDFPPVAVEGPFDKQTLDAAHVREQERLVTRGWRRLKELAHLE
                     GVSLLDYPLPEVREKMRSA"
     gene            complement(7839..8639)
                     /gene="dapB"
                     /locus_tag="VFB80_19365"
     CDS             complement(7839..8639)
                     /gene="dapB"
                     /locus_tag="VFB80_19365"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZN36002.1"
                     /translation="MSVTSIAIHGAAGRMGRRLVALASTDPQLRVAAAVDSPTHPELG
                     RDAGSLAGVKELGVLLAGSLPADAPDAVIDFSVPAAAEAVTAACISRQIPLVMATTGL
                     TEAHMERLREAARAIPVVWSPSMSMTVNLAMKLVERAGLALKGVSGGVDVEIIERHHR
                     MKEDSPSGTALKFGELIAAAMGQTIHRHGRQGQIGKRPPGEIGYHAVRTGDNPGEHTI
                     IFGLLGETLEFTVKASSRDCYAQGALAAAKFVAGRGPGMYSMYDVLGL"
     gene            9229..9447
                     /locus_tag="VFB80_19370"
     CDS             9229..9447
                     /locus_tag="VFB80_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36003.1"
                     /translation="MHAVSATLPSGPPATTVVRLSAEEIAAKTGVAATGRQPRPIAAI
                     LPQVLARYGLSQIAQSSESQPRQLDLFA"
     gene            complement(9486..10082)
                     /locus_tag="VFB80_19375"
     CDS             complement(9486..10082)
                     /locus_tag="VFB80_19375"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZN36004.1"
                     /translation="MDLATWAVLLLVLGMILLVLEFFIPSGGALAVLCALSFLGAIVL
                     GFAAGRWTGAFILLTEVVVVPAALAAAVRWWPDTPIGRMILIQRPKSSDEVLPQTLAY
                     RGLKDLVGRRGQAKGLMLPSGLVVIDGKSYDAVSDGMAIEAGQPIIVVNVSTQRLVVR
                     PDTTIQAELAGSEPAEAAAASPDPSQPLVADIPDPFAE"
     gene            complement(10213..12468)
                     /locus_tag="VFB80_19380"
     CDS             complement(10213..12468)
                     /locus_tag="VFB80_19380"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZN36005.1"
                     /translation="MRRVVRHLDIRADVAGLDLARRHPARTVRRLAWLALAGWLLGHF
                     LAPSKPAAELLAQDAAEKAKADGAAGEAVAYRFDVPLPITGKGVSRRVEQALRKLPKG
                     AQRPLFIFEFRPASGTAGEGSGFGDALDLARFLSGDQLSGVRTVAWVPRTIKGHAVLP
                     VLACEQIIMGKQAELGAAGLNEKTIDPTVRRAYSEIAERRRTVPTAIALGLLDKDLSV
                     FKVTTLEGVRYETAEELAKLRQQGAVSKEETVFQAGDQHLLTGNDMRFAFGFASHLAE
                     DRRSLATALQLPLAALQQDLTPEEGWKPIHLTLSGPIHQQAVNWIVRSIDDHQRRGDF
                     NLLVLSIDSGGGDLGESQRLAADLANLDQSIHTVAFVERQARGDAALIALACDELIVH
                     PNATIGGAGEISPDSRELAAARGGIAETFSRVGRDWSLPAALVDPGLRVHKYTHPLGG
                     EPRYLCAEEAQSLPDAAQWQREERALDTSDGLSGDKLEELGLARGTARNFEEFKAQFQ
                     LEGEIQEVRPNWALAAIEWLSDPRIAWILLFVGWFALMFELSTPGVGMPGFVAVVCFL
                     LYFWSQFLHGTAGMLEILLFVGGLICLSVEIFVMPGTGVFGVGGGLMVAVSIILASQT
                     FVVPTNAYQMRQFPVSLLMVAAGMAGGVASIYVIRRFLPDTPYLNRMLLAPPRAEERE
                     AISRRESLVAWDHLSGKRGITMTPLVPAGKVQFGDELVDCVSNGELVAKGTPVVVEEV
                     AGNRVVVRRVT"
     gene            12719..>12940
                     /locus_tag="VFB80_19385"
     CDS             12719..>12940
                     /locus_tag="VFB80_19385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN36006.1"
                     /translation="MAFINLSLLAGGLLVAIPVVLHLIMRQRPKQFIFPALRFIQQRR
                     IANQRRLQLRHWLLLALRCGAIGLFALALA"
BASE COUNT         2332 a         4444 c         4065 g         2099 t
ORIGIN      
        1 ctttacggcg tttcacgact atttcaaagg cgatctcaag ggctggacca gcgaccggta
       61 catgcccaag attcgcgacg cctacgaaaa caacccggac aagatgccct tcgactttcc
      121 tgaggtgctc gcggccatcg ccccccgcgg cgtctatgtc gccgccccgc tgcacgacag
      181 caatttcgag aacagcggcg tccgcaaatg catcgcctcc gcccagccgg tgtttgagct
      241 gcttggcgcc aaagaccaga tcgtggccga gtatcccgac tgcgggcacg attttcccga
      301 cgatgttcgc gagcgcgtgt actcgtggct ggagaagcag ctgcggaagg agtgagtggt
      361 gagtggtagg gtgggacaag gccgctgcgg ccgccggccc accatccgtg gcgcaagcaa
      421 ggtgggccgg cgctcgcgga ctcgctggtc ccaccctacg ccatcccgcg accagtggtc
      481 gcggctttga tgcgatgccc tggttcaccg aatctgcttc gtcgatttcc cgctcgcggg
      541 tcgaacagct cgtgcagcgc gctctcgatg aggccaagag ccgcatttgc aagacgccgc
      601 ggcgcgtatt gctgctgccg ccggatatca cgcggatgca ttcgggcagc ggctggatca
      661 ccgaggcgtt ctacagattg ctcaaggatt cagccgacgt gcacgtcatt ccgacgttgg
      721 gccaacacga gccgcacacc cgcgagcaga acgcccagat gtttggctcg attccgcacg
      781 agcggatcca cgcccacgat tggcgcggcg gctgtacgca cttgggagac gtgccggcgg
      841 actacgtgaa ggaggcctcg ggcggcgccg ccgattggaa gttcccggtc tggctgaact
      901 cgatgctgat gcaggagccc tgggacctga tcatcaacat cggccacgtc gtgccgcacg
      961 aagtgctggg gtttgccaac cacaacaaaa actatttcat cggcctgggg ggcaaggacc
     1021 tgatctgcgc cgcgcacatg gccgcggcct gctgcgggat cgagaacaac ctgggcaacc
     1081 tgctcacgcc cgtgcggcag tgcttcaact gggcggagga gaagtttctg gcgcgcctgc
     1141 ccgattttta cgtgcaggtg gtccttgccc gcgacgagca gggccggctc gcgcacagcg
     1201 gcgtcttcgt cggcgacgat ctggaaacct acctggccgc cgcccgacag gcccgcgagc
     1261 agaacattac gaccttcgac gagccgctgc agaaagtcgt ttgcgtcatg cagggggacg
     1321 agttcttcag cacctgggtc gccaacaagg ccgtctatcg cacgcggatg gccctggcca
     1381 cgggcggcga gctgctgatc atcgcccccg gcctgaagcg gttcggcgag caggccgacg
     1441 tggaccagct gatccgcaaa tacggctatt gcggcacgcc ccgcgtcatg gagctctacc
     1501 agcagaacgc cgacatgcag gacctggccc acgcctcggc ccacctgatc cacggcagca
     1561 gcgaaggccg cttccgcatt acctatgctc ccggccacct cgcgcgggcg gacatcgagc
     1621 aggtcaagtt cggatatgcc gatcttgcgg ccaccctccg ccgctatccc gtcgagcgcc
     1681 tgcgagaggg cttcaacgac attgacgggg agcgcatctt cttcatcccg acgccctcgg
     1741 ctggcctgtg ggccacgaag gccaagctct acgggcgcaa gtccgggttt ggcctggatt
     1801 agcgtccggt ttcgcgtcgt gcggcctcgc ccgattgtgc gcgtcgtgcg gattgtcggc
     1861 acgccttcaa aatccgacgt agggtttctg ttagccgctc gatggcgaac cagacaggtg
     1921 agtcaatctg tcgcagtgga gcattcggtc ccggcgcggg aagcgccctc ttccagcaaa
     1981 gggagtgctt cgctgacgtc cgcaatcggg cgggagcggg actcttgtcg caagggagat
     2041 tcgtcatgcg cactacatgt gcagcacagg gcaagtccgc gcgccgcggg aacatcgtcg
     2101 tgttctctgc attcttgatg gccatcttca tggccatgct ggcgctgtcg gtggacctcg
     2161 gtttcatctt cacgatgaaa gcccaactcc agcgggccgt ggacgccgcg gcactggccg
     2221 cagccggcga gctggtcaac ggcatcccgg cggctgaagc ggcggccagg gagtatctgg
     2281 tccgcaatcc tgtgggatcg gcccagggct cgatcggcgg cgacttgggc gaggccctcg
     2341 tcacctttga ggagcaacac gccgacgatt acacgttcac cgccggcaac tggaacacga
     2401 ccacgcgcac gctggaagaa accagcgtca tgccggcggc gatgaaagtc tcgatgactt
     2461 atccgaacct gccgctgttc tttgcccgcg tgatgggcca cgaccacttc tcgctgacgg
     2521 ccgaatcgac cgctatgttc catccgcgcg acatcgtggt ggtgctggac ttctcggcgt
     2581 cgatgaacga cgacagcacc ttcaacgcga tcggcaagct gccgcagtcg cagatcgagg
     2641 gcagcctgct ggagtgctgg agcgatctgg gcccgccggt gtacggcaac ctgcccgcga
     2701 cgccgacctg ggccacggcc cacggcgtgc cggcgaacat cgccacgggg ttgccgcact
     2761 gcaccgtgcg ctaccagaac accgccgtct atgtcacctc gacgctcaac ctggccacgg
     2821 tcaagctcga gttctccaac ggcgcccagc agaccttcac gccaggcgcc gtgaccaacg
     2881 gcaccttcac cggcaccggc ggcaacgcca acaaacaggt ccgcaaggtc tgggtcaagt
     2941 cgggaacgaa cgtctcgctc ggcctcaacg gcgagttctt cgacttcacc agcgccaaca
     3001 tcaatacgac gctgaagaag gccctgggcc tgaacactgt gacctatccc tatcccaacg
     3061 gcgggaactg ggacggctac atcaactact gctcgaactc gtcgaacggc aacaagaatg
     3121 ccggcttccg ctacaagttc ggcggcatga acctggtcga gtactggcta acgagcttcc
     3181 cgggctacaa ccaggtgccc gacctgtgga agacccgggc tgagccggtg tatgccttga
     3241 aggacgccaa cggcgtgttc atggacttca tcggccaggt caataccctc gaccgcgtgg
     3301 ccctggtgat ctacaacgcc gccgacggca acgcgatcat ggagagcaac ttcacccacg
     3361 atctggactc gatcacgaat atcgtgaatc atcgccaggc cggtcactat caccaataca
     3421 ccaacatcgg ggccggcctg caaaaaggcc ggatggtgat ggacagctat gcccgccaga
     3481 acgccaacaa gctgatcgtg ctgatgaccg acggcctggc gaactggcac aacggccagt
     3541 acgacctggc gggggccgcc cagatgatcg aggaagaggc caacctctgt gctgccgact
     3601 cgcggaagta caaggtgacc acggtctcgg tgggcgtcga agccgacacc gagacgatgg
     3661 ccgacgttgc caacgtgacg ggcggcaagt tctacgtcgt gccgggcggc tcgacccacg
     3721 aggcgatgca cgcccagctg aagacggcgt tcgaggagat cgccaaggcc cggccgatca
     3781 agctggtaca gtagctgcgg cctgacctcc cgttgtcgaa tctggggccg ccggaatttc
     3841 cggcggccct ttttgttgcg caaagtaggt gccgcctgcc gggcgggacc tggcagcgcg
     3901 gcaaggccga atcctcggcg atattcttgg tcgctgcgct tccctcgagg ccccttccgg
     3961 cagaaggggc ctacccgcaa gaaaagtccg gccgcgcgat tcaggatcga tcgcgcggcc
     4021 ggtttgacac ccttccatcc actcgcgggc agccaacttc ccaataggct gccccacgtt
     4081 gttcatcgag cggcggcccg ggcgcactga cgcgaaaatc ctctccaggc ggtcgatcct
     4141 gcccgaccct gacagcttgc gcgggccata gggtctgtgc aggccgcggc tcatcgttct
     4201 ttcgccttaa cggcgattca gttagattga agccttgcct cttgcgcact gggagcgttt
     4261 gccgatgaga cggcgtgtcg tcgtgacggg aatggggtgc gtcaatccgc tcggccacga
     4321 cgtcgagtcg atgtggagcg ccctccgcga gagccagtcg ggcgtcggct acacgacgat
     4381 cttcgacgcc agcaagttcc ccacgcagat ctcggccgaa gtgaaaaact gggacatctc
     4441 gcaggccggc gaggaccctc aggcctggaa ataccgggcc cggcacacgc ggttcgcctg
     4501 cggctcggcg aagcaggcca tgcggcaggc cgggctggaa ggcggtccgc ccgatccgac
     4561 gcggatgggc gtttatctgg gctgcggcga aggcaaccag gacttcctgg cgttctcgaa
     4621 gatgatgacc gccgccctcc gcccggacgg cctcgacctg gcaactttcg cccgcacggg
     4681 gctggaaatc ctcaaccccg tggccgagat cgagcaggag ccgaacatgc cggcgggcta
     4741 catcgccggc ctcttcaatg cgcaaggtcc gaactccaac tgcctgaccg cttgtgccgc
     4801 cagcagccag gcgatcggcg aagcgaccga gatcatccgc cggggcgacg ccgacctgat
     4861 gctctccggc ggcgctcaca gcatgatcca tccgttcggc gtcacggggt tcaacctgct
     4921 caccgccctg gccacgaagt acaacgacca gcccaccaag gccagccggc ccttcgaccg
     4981 cctccgcgac ggcttcgtcc tgggcgaagg cggggcgatg atcgtcctcg aggaactcga
     5041 gcacgcccgc cgccgcgggg cgccgatcct gggcgagatc atgggctacg gcacgaccgc
     5101 cgacgcctac cgcattaccg acattcaccc ggaaggccgc ggcgcgatcg cctgcatgaa
     5161 aatggccatc gccgacgccg aactgaatgc ctcggacatt caatacgtca acgcccacgg
     5221 cacgagcacc gccgtcaacg accgcgtcga aacggtcgcc tgcaagcaag tcttcggcga
     5281 gcgggcgctg caaacgcccg tctcgagcac caagagcatg atgggccacc tgatcgccgc
     5341 ggccggcgtc accgaggcga tcgtctgcct gctggcgatc cgcgacaacg tcctcccgcc
     5401 gacgatcaac tacgagaacc cggatcccga ctgcgatctc gactacgtcc ccaaccaggc
     5461 ccgcgagcag cgctgcgact acgccctctc caacagcttt ggcttcggcg gccagaatat
     5521 ctcgctggtg gtgggacggt tcagctaggc tcgtttaacc cgtagccgga ggcgcaggct
     5581 ttcccgcgaa cgcgccgtcg cctccggctc cgggttaaac gacgtcgtcg ccttcccatt
     5641 ccaccatcga tcctgtcacg cgcttgatca ggatcgtcgc gtacgagccg cgggccagcg
     5701 aaaagcgcag cgtcagcttc cgccggccgg gatacagctc gtcagccgct gccgactgct
     5761 ccaccgcttg cggcagcaag agcacggccc gttctccttt cgagaagaac gagtcgcgcg
     5821 gatacttcac ccgcagctcg cggagggcga ttccttcggc ggccagcacg cggtcgtaaa
     5881 gctcctggag cgggccgcgc tccaagtgca gccgggcaga gggcagcggc aactgcgtgg
     5941 ctcgcagccg ctcgaattgc tcgctttcca gcgcagcgat tagcggcagc tcgcgccggc
     6001 cgatggtctg cggcgcaagc tgctccggcc ggcagacttc gcgcagcagc gccgccagga
     6061 cctggttcca caactggctc tgataggccg ccagccacag gctccgcagg tcctgccgca
     6121 agaggccgat cgcccggcga aagtcgccgc gcgacgactc caggaatccc gcgactcgct
     6181 gccgctcgga tcgcgccagc aattcgccgc agcgctgcca ctggccccag tggtcgcgga
     6241 gcaatcgctt ctgctcacgg tcttccggcc ggtcgcgggc gttgggctcg gccagcgcta
     6301 gccagagcgt ccgctcataa tcgccgcggc accagggctc ggcgatgaag tggcccgact
     6361 ctcccagcga accgaaccgc tggtcgtcga agtaattggg cacgccaaat tgcgtcgccg
     6421 ctgaaagcgc cgccgacgcc gcctgtgctt cggcctcgct cagttcgcgg atcacgacgg
     6481 tgaaggcgtt ggagaggatg tcgctggcat gaatcggccg cgccgctggc cccaggtatt
     6541 ccaggcgaaa actctcctcc gagagattcc gcggcggacc gccctcgatc gtgacgaact
     6601 gccgcgtttg agcgtgcttg tctttcagcc cggcaaaggc gatccggccg cggtccagat
     6661 tccaccgccg gcgaatcgcc tcgatcgcct ccagcgtccc cagcgactct ttcgtcagca
     6721 aatacatcgc cagcggaccg ccgctgggcg cgagcgaaac ctgctcctcc acttgaaaat
     6781 cttcggccag ccgcttgagc ttcatgacgc gaggatctcc gccaccgccg tcgcagcgag
     6841 cgccagcgca cagccgtagg ccactcgcgg atcgaccggc tgtgccgccg agccgagtcc
     6901 aaaatgtcgg ccctggccgt tccacagcgc caatcccgca agcgcgccga tcgtgccgaa
     6961 tagccacagc gtccagggcg gcgcgccgct gcgcagcatg tcgcccgcgt cgccgatgcc
     7021 tccgaacgag ccgacgccga tatacaggcc gttggccagc aggcaaaaac cggcgaacca
     7081 gcgcgcgagc cacgccaggc gcagccgcag caacgcggcc agcccccaga tagccagcgg
     7141 aaggaccgaa cccaccagcg gcccgctcca agcgacgatc agcggctgcg gattcgggtc
     7201 cacgtcggtc cgcgagattg ccagcggatg caggacgacc tgcacgacct tcccgcccgt
     7261 ccaccaggcc gccgcgacgt gtccgagctc gtgcaccgcc tgcatcagca gccaggccag
     7321 caggccgccc gacacgatca acagaatcga gtgccagcgc ggcatgcttc acgcgctccg
     7381 catcttttcg cgcacttccg gcagtggata gtcgagcagc gagacgcctt ccaggtgcgc
     7441 gagctccttg agccgccgcc agccgcgagt cacgagccgc tcctgctcgc ggacgtgggc
     7501 cgcgtcgagc gtctgcttgt cgaaggggcc ctccacggcg accggcggaa aatcgggatc
     7561 gacgatgaac tgggccagcg tgggattgca gcggatgtgg cgctcccgcg tggtgtgcag
     7621 cgtctccgga tcgacctggc cgatcacgta ccgcagatac aggtcgctct cgtagatctg
     7681 ctccacgtcg ccgccgcaga ccgagcacag gtagccttct tcgcacttcg ccatacggga
     7741 acaaatccca agaaccaaag aaccgaagaa ccgaagaacc aaagaaccaa agaaccaaaa
     7801 tgctgcactc ttcctttggt tcttcggttc ttggaatttc aaagccccag gacatcgtac
     7861 atcgaataca tccccggccc gcggccggcg acaaacttcg cagcggccag ggcgccttgg
     7921 gcgtagcagt cgcggctgct ggccttgacc gtgaactcca gcgtctcgcc caagaggccg
     7981 aagatgatcg tgtgctcgcc cgggttgtcg cccgtgcgga cggcgtgata gccgatctcg
     8041 cccgggggcc gcttgccgat ctggccctgg cggccgtggc ggtggatcgt ctggcccatc
     8101 gccgccgcga tcagctcgcc gaacttgagg gccgtgccgc tgggcgagtc ctccttcatc
     8161 cggtggtgcc gctcgatgat ctcgacgtcc acgccgccgc tgacgccctt cagcgccagc
     8221 cccgcccgct cgacgagctt catcgccagg ttgaccgtca tgctcatgct cggcgaccag
     8281 acgacgggaa tcgcccgggc cgcctcgcgg agccgctcca tgtgggcctc ggtcagcccg
     8341 gtcgtggcca tcaccagcgg aatctgccgg ctgatgcagg cggcggtgac cgcctcggcc
     8401 gccgccggaa cggagaaatc gatcacggcg tcgggcgcgt cggcgggcag gctgcccgcc
     8461 agcagcacgc ccagttcctt cactccggcc agcgagccgg cgtcgcggcc gagctccgga
     8521 tgggtgggcg aatcgacggc ggccgcgacc cgcagctgcg gatcggtgct ggccagggca
     8581 accaggcggc ggcccatgcg gccggccgcg ccatgaatgg caatcgaggt gacggacatg
     8641 gatcaaccag ttttttcggc gaagtgtagt cctgtcggct gagcctatcg gacaatcggg
     8701 gcagctggca aggaatccag gttctcgtcg ccggccagcc cggtcgccgc cattagccgc
     8761 aaatcgcagg cggcagatgg cattcgaaaa ggggtaagga taattccccg cggcaacgtg
     8821 ccgtgaactt cggaattcat ctgcattctt cggcacaact tgttgtgcgg cttgtgtttg
     8881 cgatcgcccg gccgctggcg ttttgcattg cggtttgaag cactagagaa cccttgcggg
     8941 aattatcctt accctcgccg ctcagaccct cgccgaccac cttcatccag cagcggaaaa
     9001 ttctccgccg ctacacgtcg gctcgcccca ggccaccggg aaagccgctt ggccaaaatg
     9061 gcggtctccc gcgccaaaga gaactatatt gcaaataccg taccaaagta cataactgcc
     9121 agccggccag tgcgtccctg gtttgccggt attgacttgt gtacatttta ggataatgat
     9181 tacactattg aaagcgaaag gggagtttac gcgaaaggag tctgaaacat gcacgccgtc
     9241 tctgccaccc tgccatccgg cccgccggcg acgaccgtcg tgcggctgtc ggccgaagaa
     9301 atcgctgcca agaccggcgt ggctgccacc gggcgccagc cgcggccgat cgcggccatt
     9361 ctgccgcagg tcctcgccag gtacggcctg agccagatcg cccagagctc ggaatcgcag
     9421 ccgcggcagc tggatttgtt tgcctagccg acgcagccgg cctgaagcgg ccgccaccgg
     9481 ttgcgttact ccgcgaacgg atcgggaatg tcggcgacca gcggctggga ggggtcggga
     9541 gaagccgcgg ccgcttccgc gggctcgctg cccgccaatt cagcctggat cgtggtgtcg
     9601 gggcggacga ccaggcgctg cgtggagacg ttgaccacga tgatcggctg cccggcttcg
     9661 atcgccatgc cgtcgctgac ggcgtcgtag ctcttgccgt cgatgacgac caggccgctg
     9721 ggcagcatca gtcccttggc ctggccgcgg cggccgacga ggtccttgag cccgcgatag
     9781 gccagcgtct gaggcagcac ttcatccgac gacttgggcc gctggatgag gatcatccgg
     9841 ccgatcggcg tgtcgggcca ccagcgcacc gccgccgcga gggccgcggg cacgacgacc
     9901 acctccgtga ggagtatgaa ggccccggtc cagcggccgg cagcgaagcc cagcacgatc
     9961 gcccccaaaa acgacagggc gcagagcacc gccagcgccc cgccggatgg aatgaagaat
    10021 tccaggacca gcaggatcat acccagcacc agcagcagga cggcccaagt tgcaagatcc
    10081 atcgggaaaa ttccaagaac caaagaaccg aagaaccaga attccaaaca agcaattcta
    10141 tgtcaaccct ttatgcttcg cttgcgctgc aggcttcggt tctttggttc tgcggttctt
    10201 ggaatttgtt tctcacgtga cgcgtcggac gaccacccgg ttcccggcga cttcctcgac
    10261 cacgacgggc gtccccttgg ccacgagctc gccgttgctg acgcaatcga ccagctcgtc
    10321 gccgaactgg accttgcccg cgggaaccag cggcgtcatc gtgatcccgc gcttgccgga
    10381 caggtgatcc caggcgacca gcgactcgcg gcggctgatc gcttcgcgct cctccgcccg
    10441 cggaggcgca agcagcatgc ggttcagata cggcgtgtcg ggcaggaagc ggcggatgac
    10501 gtagatcgaa gccacgccgc ccgccatgcc ggctgccacc atcagcagcg agaccgggaa
    10561 ctgccgcatc tgataggcat tggtcgggac gacgaacgtc tggctggcca gaatgatgga
    10621 aacggcaacc atcaagccgc cgcccacgcc gaacacgccc gtgccaggca tcacgaaaat
    10681 ctccaccgac aggcaaatca gcccgccgac gaagagcaga atctccagca tgccggccgt
    10741 gccgtgcaga aactgcgacc agaaatagag caggaagcag acgaccgcga cgaagcccgg
    10801 catgccgacg cccggcgtgg agagctcgaa catcagcgcg aaccagccga cgaacagcag
    10861 aatccaggcg atccgcggat cggagagcca ctcgatggcc gccagggccc agttcgggcg
    10921 gacttcctga atttcgcctt ccagctggaa ctgcgctttg aattcctcga agttgcgggc
    10981 tgtgccgcgg gccaggccca gctcttccag cttgtcgccc gacagcccgt cgctggtatc
    11041 cagcgcccgc tcctcgcgct gccactgcgc ggcgtcgggc agcgactggg cttcttccgc
    11101 gcacaggtag cgcggctccc cgcccagcgg atgcgtgtat ttgtggaccc gcagacccgg
    11161 atcgaccagg gccgccggca gcgaccaatc gcgaccgacg cggctgaacg tttcggcgat
    11221 gcctccgcgt gcggcggcca gctcgcggct gtcgggcgaa atctcaccag cgccgccgat
    11281 cgtggcattg ggatggacga tcaactcgtc gcaggccagc gcgatcaggg ctgcgtcgcc
    11341 gcgggcctgt cgctcgacga acgcgaccgt gtggatcgac tggtccaggt tggccaggtc
    11401 cgcggccagc cgctggctct cacccaggtc gccgccgccg ctgtcgatcg acagcaccag
    11461 caggttgaaa tcgccgcgcc gctgatgatc gtcgatcgac cgcacgatcc agttcaccgc
    11521 ctgctgatgg atcgggccgc tcaaagtcag gtggatcggc ttccagccct cttcgggcgt
    11581 caaatcctgc tgcagggcgg ccagcggcaa ttgcagggcc gtcgccagcg accgccggtc
    11641 ctcggccagg tggctggcaa acccaaaggc gaaccgcata tcgttgcccg tcagcagatg
    11701 ctggtcgccg gcctggaaca ccgtctcttc cttgctcacg gcgccttgtt ggcgaagctt
    11761 agccagctct tcagccgttt cgtagcgcac accctcgagc gtcgtcacct tgaagacgct
    11821 caggtcctta tcgagcaggc ccagggcgat ggccgtgggc acggtccgcc gccgctcggc
    11881 aatctcggaa taggcccgcc gcaccgtcgg gtcgatcgtc ttttcgttca ggcccgccgc
    11941 gcccagctcg gcctgcttgc ccatgatgat ctgctcgcag gccagcaccg gcagcaccgc
    12001 gtgccccttg atcgtccgcg gcacccaggc cacggttcgc acgccgctca gctggtcgcc
    12061 cgagagaaac cgcgccaggt cgagcgcgtc gccgaagccg ctcccttcgc ccgcggtgcc
    12121 gctggcgggg cggaactcga agatgaagag cggccgctgc gcccccttgg ggagcttgcg
    12181 gagcgcctgc tccacccggc gcgaaactcc cttgccggtg atcggcagcg gcacgtcgaa
    12241 gcgataggcg accgcctcgc ccgcggcgcc gtcggccttg gctttctcag cggcgtcctg
    12301 ggccaacaat tcagccgcag gcttgctcgg cgcaaggaaa tgccccagca gccagcctgc
    12361 caaggccagc cacgccagcc gccgtacggt ccgcgccggg tgccgccgcg cgaggtcgag
    12421 gccggctacg tcggcacgaa tgtcaagatg tctcacaacg cgacgcattc agcacctctc
    12481 cgtagctggc gctcggtttt gacaaccaag cgaatcctcg ttcccggcaa ttataggccc
    12541 tgccgagacg agaaaaaatc ctcgccgcaa agtcttcaca cgccgcccct tagcgggctc
    12601 gccagaccca cgggcgggcc agccgccggc tgtctattcg ttcggcggcc gcctttgttg
    12661 gccgcacgcc gcgggccgcg gcgggctatc atggaagctc gggcacggga agaggttcat
    12721 ggcgttcatc aatctgtcgc tgctggcggg ggggctgctg gtcgccatcc cggtcgtgct
    12781 gcacctgatc atgcggcagc ggcccaagca gttcatcttt ccggccctgc gattcatcca
    12841 gcagcggcgg atcgcgaacc agcggcgatt gcagctgcga cattggctgc tgctcgccct
    12901 gcggtgcggg gcgatcggcc tgtttgcgct ggcgctggcc
//
LOCUS       DASRJB010000736         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3877917, whole genome shotgun sequence.
ACCESSION   DASRJB010000736 DASRJB010000000
VERSION     DASRJB010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877917"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1279
                     /gene="miaA"
                     /locus_tag="VFB80_19390"
     CDS             221..1279
                     /gene="miaA"
                     /locus_tag="VFB80_19390"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZN36007.1"
                     /translation="MPDDLHPAPVPPALDCWYLTGPTAAGKTKVGDELALRLNAEIIS
                     LDSMALYQGLDIGTAKPAPEDRAAVPHHLLDLVPPTEEFSLSQYIDVAHAAIAEIRSR
                     GKEVLFVGGTPLYLKSLLRGVFQGPPADWDFRKTIEKELETLPLEALHQRLQVIDPLL
                     AAKLHPNDKRRIIRGLEVFKLTGQRLSHLQTQFDEGRPASAVKVFVLAWPRDELHRRI
                     DARVDQMFAAGLVEEVRGLVTRYGTLSRTAVQAVGYQEVIEHLEGVGWVKADAAADPR
                     GVPPGEPAPACVIGCGSLDTPYEDCVARVKARTRQFARRQETWFRSLSECTLIPMQPG
                     VPPPAVAAKIALTATTRG"
     gene            1295..>2156
                     /locus_tag="VFB80_19395"
     CDS             1295..>2156
                     /locus_tag="VFB80_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36008.1"
                     /translation="MSSPDDPPKRGWRSIRFTLGELFLLLAGLALGLAYARVDGSDRW
                     HDFLLAIFGGCFVVGLVQKSWRTWSLRRGDSTISFIDAIWPLAVAVLMVFTGVMQAAA
                     RHNWWAFQDFNGDTFWGTGDLTDTAFYLAVICGYFQLRETVPAQGWRRWMALLLNATA
                     LLLGAYWLLVLLEEGMLLIALVHTAIQGVELAMPTRWAGKPFYPANLHTELTRQFVAR
                     AYWTAALLAGAVVANIALARTWSRGWSQRVLWSVVCTLLLIVAWRLDWWCVRVGFPTL
                     SPFIYSVVDTL"
BASE COUNT          347 a          714 c          677 g          418 t
ORIGIN      
        1 ccggcgacgg cgtcagcaag ctccaagagt cgctgccggg attggccgac gtgcagcgtt
       61 agtcagggaa ggcaaccacg aatcgcacga atgacacgaa tcaggcagtg aattcgcggc
      121 ggaccgaacg ctttgggaac gctttcgatt ccccgtaccg ttgaggtccg cagtccaaaa
      181 tgcctattgt cctattcgtg gttttccctg ccctgtatca atgcccgacg accttcaccc
      241 cgcccccgtc cctcccgcac tcgactgctg gtatctgacc gggccgaccg cggcaggcaa
      301 gacgaaggtg ggagacgagc tggccctgcg gctgaatgcc gaaatcatct cgctcgactc
      361 gatggccctc tatcagggac tcgatatcgg cacggccaag cctgcgccgg aagaccgggc
      421 cgcggtgccg catcacctgc tcgacctcgt tccgccgacc gaggagttca gcctcagcca
      481 gtacatcgac gtcgcccacg ccgcgatcgc cgagattcgc agccgcggca aggaagttct
      541 cttcgtcggc ggcacgccgc tctacttgaa gtcgctgctc cgcggcgtct ttcaaggccc
      601 gcccgccgat tgggattttc ggaagacgat cgaaaaagaa ctggaaacgc tgccgctgga
      661 agccctgcac cagcggctgc aagtcatcga cccgctgctg gcggcgaagc tgcacccgaa
      721 cgacaagcga cggattatcc gcggcctgga agtcttcaag ctgaccggcc agcggctcag
      781 ccacctccag acgcagttcg acgaaggccg cccggcttcc gccgtcaagg tgttcgtgct
      841 ggcctggccg cgcgacgaac tgcaccgccg gattgacgcc cgcgtggatc agatgttcgc
      901 cgccggcctc gtggaagaag ttcgcggcct ggtcacccgc tacggcacgc tcagccgcac
      961 ggccgtgcaa gcggtcggtt atcaggaagt gatcgagcat ttggagggcg tagggtgggt
     1021 caaggccgac gcggccgcag acccacgcgg cgtgccgcca ggcgaacccg ctcccgcgtg
     1081 tgtcatcggc tgcggcagcc tcgacacacc ctacgaggac tgcgtcgccc gcgtgaaggc
     1141 ccgcacgcgg cagttcgccc gccggcagga gacctggttc cggtcgctga gcgaatgcac
     1201 gctcattccg atgcagccgg gcgtgccgcc gccggccgtc gcggctaaga tcgccctaac
     1261 ggcgacgacc cgcggctgac tttcttgtga caacatgtcc agccctgacg atcctccgaa
     1321 gcgcggctgg cgctcgattc ggttcacgct cggcgaactc ttcctgctgc tggccggcct
     1381 ggccctcggc ctggcctacg cccgcgtgga cggcagcgac cgttggcacg atttccttct
     1441 ggccattttc ggcggctgct tcgtcgtcgg cctggtgcaa aagtcctggc gaacgtggtc
     1501 tctccgccgc ggcgattcaa ccatctcctt catcgatgcc atctggccgc tggccgtcgc
     1561 agtgctgatg gtcttcactg gcgttatgca ggcagctgcg cggcacaact ggtgggcctt
     1621 tcaggacttc aatggcgaca cattttgggg cacgggtgac ctgacagaca cggcgttcta
     1681 tctggctgtg atctgcggct acttccaact ccgcgagacg gtccctgcgc aaggttggcg
     1741 gcgctggatg gcgctgcttt tgaacgccac agctttgctg ctgggcgcct attggctatt
     1801 ggtcctgttg gaggagggga tgctcctaat tgcgctcgtt cacaccgcca ttcagggcgt
     1861 ggaactggcg atgccaaccc gctgggctgg caagccgttc tatccggcga acctgcacac
     1921 cgaactgacg cggcagttcg ttgcccgcgc gtattggacg gccgcgctat tggcaggtgc
     1981 cgttgtggca aacatcgcgc tggctcgcac ctggtcgcgc ggctggtcgc agcgcgtcct
     2041 atggtccgtg gtttgcacgc tcttactgat cgtggcctgg aggctggatt ggtggtgtgt
     2101 gcgcgttggc tttcccaccc tgtcgccgtt catctattcc gtggtggata ctcttc
//
LOCUS       DASRJB010000737         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3317355, whole genome shotgun sequence.
ACCESSION   DASRJB010000737 DASRJB010000000
VERSION     DASRJB010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317355"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFB80_19400"
     CDS             <1..363
                     /locus_tag="VFB80_19400"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36009.1"
                     /translation="KFDETSARYHGTRPVVADLEPELTARLQRVALDAYRALRVRDYG
                     RIDLRLTESGEIYVIEVNASCYLEQEGEFVMAAEAHGMTCNELIGRIADLAIERWKRR
                     SVARRQKRKRPTARATAV"
     gene            complement(377..1429)
                     /locus_tag="VFB80_19405"
     CDS             complement(377..1429)
                     /locus_tag="VFB80_19405"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HZN36010.1"
                     /translation="MPASFRESNLFGTPIRDLKLAIEGTPLAPVIEEFRGELRAKGLT
                     RVSPKFHLSTEWGVPFGTVVIGIPFYLARPELTELHGEEVGHVEGFSRQDILRYLRHE
                     MGHVINYAYKLYNDEEWVKLFGSITQPYVEDYRPKPFSRRFVRHLPGWYAQKHPDEDW
                     AETFAVWMTPDRDWQSEYPAGSGAHIKLDYCDRALARLREQEPLVTATELDQDVGELT
                     FSLSDYYRDQPGDGEPSPGLDGDLRAIFDDLAEAAARNPSGETRSASGLIRSLERQLA
                     ADVFRWTGHFPEKTRRLMRHLAERAAALEQVYPASAETEAIIGVTILVTSLAMNHVHR
                     GSYFPETQPPADRSGR"
     gene            complement(1534..>3120)
                     /locus_tag="VFB80_19410"
     CDS             complement(1534..>3120)
                     /locus_tag="VFB80_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN36011.1"
                     /translation="RFATDFDLQNWLRAQLMFEDVLSRLGLGSKSFSSYGGGWNEGTG
                     GDELVSLNPATGQPLGKVAAATALEYDQVVETSRQAFATWRLVPPPQRGDIVRRIGNA
                     LREYKRDLGLLVTLETGKIKSEGLGEVQEMIDMCDFAVGLSRQLYGLTIASERASHRM
                     LEQWHPLGPVGCITAFNFPVAVWAWNAAVALVCGDPVVWKPSELTPLTALAVQHIVND
                     VLEPAGLAGALNLVLGGREIGQKMAADVRLPLISATGSCGMGRAVGSAVAARMGRSLL
                     ELGGNNALIVAPSANLDLALRAIVFSAVGTAGQRCTTLRRLILHHSLVDKLLPRLVAV
                     YKSIRPGNPWDDGVLLGPLIRESSVQQFEQAVKTAQAQGGEVLCGGERIDRPGFYVEP
                     TIILGRKDGLGMEIVKQETFAPILYVLPYGGEFDTLDHAIYLHNSVPQGLSSAIFTDR
                     FQDAEAFLSTAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQT
                     CTLNFGQDLPLAQGVKFDVI"
BASE COUNT          538 a         1055 c         1006 g          521 t
ORIGIN      
        1 aagttcgacg agaccagcgc ccgctatcac ggcacgcgac ccgtcgttgc cgacctcgag
       61 ccggagctca cggcgcggct gcagcgggtc gcactcgacg cctatcgtgc cctccgcgtc
      121 cgcgattacg gccgcatcga cttgcggctc accgagagcg gcgaaatcta cgtcatcgaa
      181 gtcaacgcca gctgctatct ggaacaggag ggggagttcg tcatggcggc cgaggcgcac
      241 gggatgacct gcaacgagct gatcggccgg atcgccgacc tggcgatcga gcgctggaag
      301 cgccgctcgg tggccaggcg acagaagcgc aagcggccga cggcccgggc gacggccgtg
      361 tgaccaggct ggcccgctaa cgaccagagc ggtcggcagg aggctgcgtt tccgggaaat
      421 agctgccgcg atggacgtgg ttcatcgcca gcgacgtaac caggatcgtc acgccgatga
      481 tggcttcggt ctcggccgac gcgggataga cctgctccag ggccgcggcc cgctccgcca
      541 gatgccgcat cagccggcgg gtcttttccg ggaaatggcc ggtccagcgg aagacgtcgg
      601 cggccagctg ccgttccagg ctgcggatca ggccgctggc gctgcgggtc tcgcccgacg
      661 ggttacgcgc agccgcctcg gccaggtcgt cgaaaatcgc ccgcaagtcg ccgtccagtc
      721 ccggcgacgg ctcgccgtcg cccggctggt cccggtagta atccgagagc gaaaaagtca
      781 actccccgac gtcctggtcg agttcggtcg ccgtgaccaa tggctcctgc tcgcgcaggc
      841 gggccagagc gcggtcgcag tagtcgagct tgatgtgggc gccggagccg gcggggtatt
      901 cggattgcca atcgcgatcg ggggtcatcc aaacggcgaa cgtctcggcc cagtcctcgt
      961 cggggtgctt ttgcgcgtac cagccgggca ggtgccgcac aaaccgccgg ctgaacggct
     1021 tcggccggta gtcctcgacg tagggctgcg tgatcgagcc aaagagcttc acccattctt
     1081 cgtcgttgta gagcttgtag gcgtagttga tgacatgccc catctcatga cgcagatagc
     1141 ggaggatgtc ctgccggctg aagccttcca cgtggccgac ttcctcgccg tgcagctcgg
     1201 tcaactccgg ccgagccagg taaaagggga tgccgatcac cacggtgccg aacggcacgc
     1261 cccattcagt cgagaggtgg aacttgggcg agacccgcgt cagccccttg gcgcgcagct
     1321 cgccgcggaa ctcctcgatc accggcgcga gcggcgtgcc ttcgatcgcg agcttgaggt
     1381 cgcggattgg cgtgccgaag aggttcgatt cgcgaaagct ggcgggcatt gcaggggtag
     1441 gggtagtggg tagggctagg gaaagggcaa acacgcttct tttcgccgcc ctagccctac
     1501 ccccagccct tcccaacgca aaccgtatgc cagctaaatc acgtcgaatt ttacgccctg
     1561 ggccagcggc aggtcctggc cgaagttgag cgtgcaagtc tggcggcgca tgtaggcctt
     1621 ccaggcgtcg ctgccggctt cccggccgcc accggtttcc ttctcgccgc caaaggcccc
     1681 gccgatctcc gcgccgctgg tgccgatgtt cacgttggcg atgccgcagt cgctgcccgc
     1741 ggtggagagg aacgcctcgg cgtcctggaa gcgatcggtg aagatcgcgc tggagagccc
     1801 ctgcggcacg ctgttgtgca aatagatcgc gtggtccaac gtgtcgaatt cgccgccgta
     1861 gggaaggaca tagaggatcg gtgcgaacgt ctcctgcttg acgatctcca tccccaaacc
     1921 gtccttgcgt ccgagtataa tcgtcggctc aacatagaat cccggccggt cgatccgctc
     1981 tcctccgcac agcacttcgc cgccttgggc ctgggcggtt ttcacggcct gctcgaattg
     2041 ctgcaccgac gattcgcgaa tcagcggtcc cagcagcacg ccatcgtccc agggattgcc
     2101 cggccgaatg ctcttgtaga cagcgacgag ccgcggcagg agcttatcga cgagggaatg
     2161 gtgcaggatc agccgcctga gcgtcgtaca gcgctggccc gccgtaccga cggccgagaa
     2221 cacaatggcc cgcagcgcca agtcgaggtt ggccgagggc gccacgatca gggcgttgtt
     2281 gccgcccagt tccaggagcg agcggcccat ccgtgcggcc accgccgagc cgaccgcgcg
     2341 gcccatcccg cagcttccgg tcgccgagat cagcggcaat ctcacatcgg cggccatctt
     2401 ctggccgatc tcgcgcccgc cgaggaccag attcagcgcg ccggccagcc cggcgggctc
     2461 cagcacgtca ttcacgatgt gctgcaccgc gagcgcggtc agcggtgtca gctcggacgg
     2521 cttccagacg accggatcgc cgcagacgag cgccaccgcc gcgttccagg cccagacggc
     2581 gaccggaaaa ttaaaggccg tgatgcagcc gacggggccg agcggatgcc actgctccag
     2641 catccggtgg ctggcccgtt cgctggcaat cgtcaggccg taaagctgcc gcgacaggcc
     2701 gacggcgaag tcgcacatgt cgatcatttc ctggacttcg cccagccctt cgctcttgat
     2761 cttgccggtt tccagcgtca ccagcagtcc cagatctcgc ttgtactcgc ggagcgcgtt
     2821 gccgatccgc cgaacgatat ctcccctctg cggcggcggc accaaccgcc aggttgcaaa
     2881 cgcctgccgc gaggtctcaa cgacctggtc gtattccagc gccgtcgcgg ccgcgacctt
     2941 ccccaagggc tgtccagtgg ccgggttcaa cgagaccagc tcgtccccac cggtcccctc
     3001 gttccagccg ccgccgtacg agctgaagct cttggagccg aggccaagcc gcgatagcac
     3061 gtcttcaaac atgagttgtg cccttagcca attctgcaaa tcaaaatcgg tagcgaacct
//
LOCUS       DASRJB010000738        10053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_778661, whole genome shotgun sequence.
ACCESSION   DASRJB010000738 DASRJB010000000
VERSION     DASRJB010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778661"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..398)
                     /locus_tag="VFB80_19415"
     CDS             complement(51..398)
                     /locus_tag="VFB80_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HZN36012.1"
                     /translation="MLRLSRLFTLIAIAYGLALAGCGSQPAPVPGTPVAADGHSHDGW
                     WCAEHGIPEGECAQCNAKLAAEFQKNGDWCEKHNRPDSQCFICHPEHAARFALKYEAK
                     YGHQPPKTPTATP"
     gene            complement(422..3574)
                     /locus_tag="VFB80_19420"
     CDS             complement(422..3574)
                     /locus_tag="VFB80_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZN36013.1"
                     /translation="MLNWIIDISLRFRWLVILVFLGAAGIGAAALTQIDIDAFPDTTP
                     VQVQINTVAPALAPEEVERQITIPVEQAISGLPRLTQMRSLSKFGLSQVVVVFEDGTD
                     IYFARARVSEQLAAVELPAEIAAPEMGPVATGLGEVFYYIVTGQGTDATDLRTIHDWV
                     IRPPLKTVRGTAEVNSWGGYEKQFQIRLDPQRLIKHDLTFDEVLAAVSQNNLSVGGGN
                     IQQHGGMLLVQGLGRTATIDEIRQIVVVSHDGVSVRLAELGDVVIGHEIRRGAVTANG
                     QGEVVLGQGFMRIGENTHKVTWALKEKLKSLSANLPANVQVRPVYDRTELVGFVIDTV
                     RNNLFEGGLLVVAVLFAFLGNLRAAAIVALAIPLSLLFAFAGMWRFGIAASLLSLGAI
                     DFGMVVDSSVVMIENCVRHLAHAGDISKKTRLQIIRDAAVEVRRPTLFGELIIMIVYL
                     PILTLTGIEGKLFRPMALTVIFALAGSMVISLTLMPVLASFLLPKKIAEREPLLMRVA
                     HAIHNPILRFAMHHKLAVLGLAASVLIVAFGMIAPHLGSEFVPKLSEGAIVIGVVRLA
                     GTSLDESIRYNTQMEQAILREFPDEVQHVWSQTGSAEVATDPMGVELTDIFLALRPRE
                     RWKKAKNQAELTEQLNLLLRDLPGQRLSFLQPIEHRMSEMVSGVRADLGVKLFGDDFT
                     VLAEKGGQIQQVLSSIKGAADVNTEQITGQPVLQIKVNQEAIARYGVPAKAVLDVVQS
                     LGSKPVGEVVEGQMRFPLVVRLPEEYRTSADAVRSLLIATPAGERIPLSRLASVEVVE
                     GPSTITREWGQRRLTISANVRGRDLGSFVAEAQRRIAREVALPAQGRYRVEFGGQFEN
                     LISARQRLLIVVPIALLLIFVLLYFTYFNVLDALRVFTSIPFAWVGGIFALWLRDMPF
                     SISAAVGFIAMSGVAVLDDMILVSYIRQLREQGLSVDDAVTKAAVTRLRPVLMTTLVA
                     ALGFLPMAVSDGVGAEVQRPLATVVIGGVIGAMVMSLLVIRVLYLVFNTAGEFFAGHG
                     EIDTPVRAEVAEPELVSSNS"
     gene            complement(3710..5302)
                     /locus_tag="VFB80_19425"
     CDS             complement(3710..5302)
                     /locus_tag="VFB80_19425"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN36014.1"
                     /translation="MATITTPTTPPIAAAQGWLAWITRALPNALVLCLLAAIFAIGHR
                     YHWKLPKLSALLAGAPAAADDWCDEHLVPESICLECRVDPKAQAKPIDWCAKHGVAEC
                     VICHPERAQTKTAPQLPAYDTAAAIGVRPRAANNSLSTLHTKVVQFASPEAVDKAGVA
                     VDVVGTAAMREEIAANGEVVFDPTRVAHLSPRVAGTVWQVYKQLGQEVHAGELLALID
                     AAAVGQSKAELLQAAATLKARQANVDRLRGIKDVVANKALVEAESALREAEIKVVSAG
                     QSLVNLGFQVPAKLHGGDAESLADEIRYLGIPEEMIATVKEQTQTANLFPLVAPIGGV
                     VVVAEIVRGEFVSTTTPAFVVADPQKLWLNLSVRQEDAPLVQLGQRVQFETENQPRAA
                     EGKIDWISPTIDHKSRTLPIRVSLDNAAGQLRDNTFGVGRIILREESQSIVVPKSAVQ
                     SAGDVQLVFVRDKRYFEKDAPKFFHVRQVRVGAKSDKQVELLAGVLPGEVVATTGSNV
                     LVSQLLRSQLGAGCGCHDGHSH"
     gene            complement(5363..5776)
                     /locus_tag="VFB80_19430"
     CDS             complement(5363..5776)
                     /locus_tag="VFB80_19430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN36015.1"
                     /translation="MRANLGVVCALAVAGAVPAIAADPVKANEQILALAKERLAIVTQ
                     LHEMTLAGYRVGTTSLSEVLEARQSLASAKLDLCQSKEERVKVFEEMVQIAEQTIDLT
                     ERLARAGETPQYKVLQAKAHFLQARINLEREKVAP"
     gene            complement(6460..6900)
                     /locus_tag="VFB80_19435"
     CDS             complement(6460..6900)
                     /locus_tag="VFB80_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36016.1"
                     /translation="MRRMRYRPRLCFGIAVIVISMMPALRLRADEVPPLTNEELGTQQ
                     QQALLLALAGDPYGHRAADHCRAGWPMLIRHHAIPSNTRHYGGYWIGGGVPLFGDSPS
                     LVEGTFGWDYFGAMNKWIDLSWSHGRRYQGGGGAYRTDGPKLPK"
     gene            complement(6904..9396)
                     /locus_tag="VFB80_19440"
     CDS             complement(6904..9396)
                     /locus_tag="VFB80_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN36017.1"
                     /translation="MSAVYRPGLRRRKLLPRPARRLSAERLEPRHLLAADAPSLTADE
                     FASFAPHHNSTSPCDVDASGELSPLDALLVINHLNQTNPAPADFYADVNADGAITPAD
                     ADWVLAALGASLSARAEPAAILPLDDEAEAEPPLPIVPPPPPIPPGDAGLNYITAAEV
                     ETLLARAAGATSTQDAIIAIVDRGGRILGVRMEQGVLDAIPDLLTRVFAIDGAVAKAR
                     TAAFFANNQAPLTSRTIRNLSQSTITEREVESNPTVPVPGDPGQNPFFNPNPISRTFG
                     PGVVAPIGVGGHFPADIAHTPPVDLFRIELQSRDGLTLPGLDGIKGTLDDIDLDNRFN
                     IPDAFLAPGVEIPAPESYGVQSDLVPHAIGRGLATLPGGIPLYKSIGGALTLVGGIGV
                     FFPGPDGYATHEQGFVPNIKQTSAQRLNAPKALEAEYVAIAAAGGVTGVRPIGAIGGL
                     SPPVGYVLPFGRIDLVGIQLEVFGPNPTRKVKTPGIDTVIRRGQALGVGSTTTGENRQ
                     IDSMGNLAQDGKPLAEGWLVLPHDSPLVGPNQIKQADVEAIVTNAITGANQIRAAIRL
                     NVTKSPPTPGVPTKMVIAVADTAGNVLGLYRMPDATFFSIDVAIAKARNTAYYADPAA
                     LQTVDMADDDLLIARGATTVAQLRKLKYRNDNGVLGFPDLYRGSKNAARYSPLTGLAF
                     TNRTFRFLAEPRYPAGVDGSLPPVFSILTDPGINRKTAENASQANPTPASWFISVMGF
                     DAFHASRNFHDPSDLANQNGIVFFPGSTPLYVSSGLAGGLGVSGDGVDQDDVVTSYAQ
                     YSYAPPQHLRADMTFYRNVRLPFQKFNRNPKL"
     gene            complement(9482..9694)
                     /locus_tag="VFB80_19445"
     CDS             complement(9482..9694)
                     /locus_tag="VFB80_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36018.1"
                     /translation="MKTRGQNQRSSKRRCDTRAKSSFSPQQHHGDSQRTCRRTRAMRL
                     WQAVSIRSTLPQEAVEPNVPIFPADA"
     gene            9788..>10053
                     /locus_tag="VFB80_19450"
     CDS             9788..>10053
                     /locus_tag="VFB80_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36019.1"
                     /translation="MPLPSFTAQDEYFPPLLWPVDPPLGYTGPSGIVPREAQTSSHFV
                     PMEDRWRIGFPEWDRYGWGHPLLEEYPSVPGAWYDPYHQNVLKG"
BASE COUNT         1687 a         3224 c         3383 g         1759 t
ORIGIN      
        1 cgaagcgaac cgcgagctcg gcagttttcg ttatatcgcc caccgctcgc ttagggcgtt
       61 gccgtcggcg ttttcggcgg ctggtggccg tacttggctt cgtatttgag agcgaacctg
      121 gcggcgtgtt cggggtggca aatgaagcat tgcgaatcgg gccggttgtg cttttcgcac
      181 cagtcgccgt tcttctggaa ctcggcggcg agcttggcat tgcactgcgc gcactcccct
      241 tcggggatgc cgtgctcggc gcaccaccag ccgtcgtggc tgtggccgtc cgcggccacg
      301 ggcgttcccg gaaccggcgc gggctgactg ccgcagccgg caagcgcgag gccgtaggcg
      361 atggcaatca aggtgaacaa gcgagagaga cgcaacatat caagctccta aggaaacgtg
      421 gtcaggagtt actggaaacg agctccggct cggcgacttc cgcccgcacg ggcgtgtcga
      481 tttcgccgtg gccggcaaag aactcaccgg ccgtgttgaa cacgaggtac aggacgcgaa
      541 tcaccaagag cgacatcacc atcgccccga tcacgccgcc gatgacgacg gtcgccagcg
      601 gccgctgcac ttccgcgccg acgccatcgc tcaccgccat cggcaaaaaa ccgagggcgg
      661 cgacgagcgt ggtcatcagc acgggtcgca gccgcgtgac ggcggccttg gtcacggcgt
      721 catccaccga caggccctgc tcgcggagct ggcggatgta cgagacgagg atcatgtcgt
      781 cgagcacggc cacgcccgac atggcgatga agccgacggc cgccgagatc gagaacggca
      841 tgtcgcggag ccagagcgcg aaaatgccgc cgacccaggc gaacggaatg ctggtgaaga
      901 cgcgcagcgc gtcgaggacg ttgaaatacg tgaaataaag gaggacgaag atcaacagca
      961 gcgcgatggg cacgacgatc agcagccgct ggcgggcgct gatcaggttc tcgaactggc
     1021 cgccgaattc gactcggtag cggccctgcg cgggcagggc gacctcgcgg gcgatcctcc
     1081 gctgggcctc ggcgacgaag ctgcccaggt cgcggccgcg gacgttggcg ctgatcgtca
     1141 gccgtcgctg gccccattcg cgggtgatcg tcgagggccc ttcgacgacc tccacgctgg
     1201 ccagccgcga gagcggaatc cgctcgccgg ccggcgtcgc gatgagcagc gagcggacgg
     1261 cgtcggcgct ggtgcgatat tcctccggca gccgcacgac gagcgggaac cgcatctgcc
     1321 cctcgacgac ctcgccgacg ggcttgctgc ccagcgactg cacgacgtcg agcaccgcct
     1381 tggccggaac gccgtagcgg gcgatagcct cctggttgac cttgatctgc agcaccggct
     1441 gcccggtgat ctgctcggtg ttcacgtcgg ccgcgccctt aatcgaactc aagacctgct
     1501 ggatctggcc gcccttctcg gccagcacgg tgaagtcgtc gccaaacagc ttgacgccca
     1561 ggtcggcccg cacgcccgag accatttcgc tcatgcggtg ctcgatcggc tgcaggaacg
     1621 agagccgctg gcccggcagg tcgcggagga ggaggttaag ttgctcggtg agctccgcct
     1681 ggtttttggc tttcttccag cgctcgcgcg ggcggagggc gaggaagatg tcggtcagct
     1741 cgacgcccat cgggtcggtg gcgacttcgg ccgagccggt ctggctccag acgtgctgga
     1801 cctcgtccgg gaactcgcgg aggatcgcct gctccatctg cgtgttgtag cgaatcgatt
     1861 cgtcgaggct ggtgcccgcc aggcggacga cgccgatcac gatcgcgcct tcagagagct
     1921 tgggcacgaa ctccgagccc aggtgcgggg caatcatgcc gaacgccacg atcaggacgc
     1981 tcgccgccag tccgagcacc gccagcttgt ggtgcatggc gaagcggaga atcgggttgt
     2041 ggatcgcgtg ggccacgcgc atcagcagcg gctcgcgctc ggcgatcttt ttgggcagca
     2101 ggaagctggc cagcacgggc atcagcgtca gcgagattac catcgagccg gcgagggcga
     2161 agatcaccgt cagggccatc gggcgaaaga gtttcccttc gatgccggta agtgtgagga
     2221 tcggcaggta gacgatcatg atgatcaatt cgccgaagag cgtcgggcgg cggacttcga
     2281 ccgcggcgtc gcggatgatt tgcaggcggg tctttttgct gatgtcgccc gcgtgagcga
     2341 ggtgccgcac gcagttctcg atcatcacca ccgagctatc gacgaccatg ccgaagtcga
     2401 tcgcgccgag gctgagcagg ctggccgcaa tgccgaaccg ccacatgccc gcgaaggcaa
     2461 acagcagcga gagcggaatc gccagggcca cgatcgccgc ggcccgcagg ttgccgagga
     2521 acgcgaacag caccgcgacg accagcaggc cgccctcgaa caggttgttg cggaccgtgt
     2581 cgatgacaaa gccgaccaat tcggtgcggt catagaccgg ccgcacttgc acgttggccg
     2641 gcaggttcgc gctgagcgat ttgagcttct ccttaagggc ccaggtgact ttgtgcgtgt
     2701 tttcgccgat ccgcatgaag ccctggccga ggacgacttc cccctggccg ttggcggtga
     2761 ccgccccgcg gcggatttcg tggccgatga cgacgtcgcc cagctcggcg aggcggacgc
     2821 tcacgccgtc gtgcgagacg acgacgatct gccggatttc gtcgatcgtc gccgtgcggc
     2881 ccaggccctg cacgaggagc atgccgccgt gctgctgaat gttgccgccg ccgacgctga
     2941 ggttgttctg gctgaccgcc gccagcactt cgtcgaacgt caggtcgtgc ttgatcagcc
     3001 tttgtgggtc gaggcgtatt tgaaattgtt tttcgtagcc gccccagctg ttgacttccg
     3061 ccgtgccgcg gaccgtcttg agcggcgggc ggatgaccca gtcgtggatt gtccgcaagt
     3121 cggtggcgtc ggtgccctgc ccggtgacga tgtagtagaa gacttcgccc aggcccgtgg
     3181 cgacgggacc catttcgggc gcggcgatct cggccggcag ctcgaccgcc gccagctgct
     3241 cgctcacgcg ggcccgggcg aagtagatgt cggtgccgtc ttcgaaaacc accacgacct
     3301 gcgacagacc gaatttcgag agcgaccgca tctgcgtcag ccgcggcagg ccgctaatcg
     3361 cctgctcgac ggggatcgtg atctgccgct cgacctcctc gggagcgagg gccggcgcga
     3421 cggtgttgat ctgcacctgc accggcgtgg tgtcggggaa ggcgtcgatg tcgatctgcg
     3481 tgagcgcggc cgcaccaatc cctgcggcgc ccaggaacac caggatcacc agccagcgaa
     3541 accgcagcga gatgtcgatg atccagttga gcatattcgc acttactcgg ttctagctcc
     3601 cttggtccca agctccgctt gggaccacac gtcgcgatgc tctgcttcgc aaccgggaag
     3661 cggagcttcc gacctggcgt gcccaagcgg agcttgggca cgaggcaacc taatgcgagt
     3721 gcccgtcgtg gcagccgcaa ccggcgccga gctggctccg cagcagctgc gagaccagca
     3781 cgttgctgcc ggtcgtggcg acgacctcgc cgggcagcac gccggccagc agctccactt
     3841 gcttgtcgct cttggccccc acgcggacct gccgcacgtg gaagaacttg ggcgcgtcct
     3901 tctcgaaata gcgcttgtcg cggacaaaca ccagctgcac gtcgcccgcc gactgcaccg
     3961 cactcttggg cacgacgatc gactgcgact cctcgcggag gatgatccgc cccacgccaa
     4021 acgtgttgtc ccgcagctgg cccgcggcgt tgtcgagcga cacgcggatc ggcagcgtgc
     4081 ggctcttgtg gtcgatcgtc gggctgatcc agtcgatttt cccttcggcc gcccgcggct
     4141 gattctcggt ttcgaattgc acccgctggc cgagctgcac cagcggcgcg tcttcctgcc
     4201 gcacgctcaa gttgagccac agtttttgcg gatcggcgac gacgaaggcg ggcgtcgtcg
     4261 tgctgacaaa ttcgccgcgg acgatctcgg caacaacgac cacgccaccg atcggcgcga
     4321 cgagcggaaa caggttggca gtctgggtct gctccttgac ggtcgcgatc atttcctcgg
     4381 gaatgcccag atagcggatt tcgtcggcca gcgattcggc gtcgccgccg tgcagcttcg
     4441 cgggcacctg gaagccgagg ttgacgaggc tctgcccggc cgaaacgacc ttgatctccg
     4501 cttcgcggag ggccgattcg gcttcgacga gcgccttgtt cgcgacgacg tccttgatcc
     4561 cgcggagccg atcgacgttg gcctgccggg ccttgagcgt cgctgccgct tggagcagct
     4621 cggccttaga ctgacccacg gctgcggcgt caatcagcgc cagcaactcg ccggcgtgca
     4681 cctcttggcc cagctgtttg tagacctgcc agaccgtgcc ggcgacgcgc ggcgagaggt
     4741 gcgccacgcg ggtcggatcg aacacgacct cgccattggc tgcgatctct tcacgcatgg
     4801 cagccgtgcc gacgacatcg acggcgacgc ccgccttatc gacggcttcc ggcgaggcga
     4861 attgcacgac cttcgtgtgc agcgtgctga ggctgttgtt cgcggctcgc ggccgcacgc
     4921 cgatcgccgc cgccgtgtcg taggccggca attgcggcgc ggtcttggtc tgagcccgct
     4981 cggggtggca gatcacgcac tccgccacgc cgtgcttcgc gcaccagtcg attggcttgg
     5041 cctgggcctt cggatcgacg cggcactcca ggcagatgct ctccggcacc aggtgctcgt
     5101 cgcaccaatc gtcggcagcc gcgggcgccc ctgcgagcag cgccgaaagc ttgggcagtt
     5161 tccagtgata gcggtggccg atcgcaaaaa tcgccgccag gaggcacagc acgagggcat
     5221 tcggcagtgc gcgggtgatc caggcgagcc aaccctgtgc ggcggcgatc ggcggagtag
     5281 tgggggtagt gatggttgcc atgacggtcg ttatgaaatg ggctgttgtc gaattggtaa
     5341 tttcagcggc gggcccgatc gttcagggcg ccactttctc tcgctccagg ttgatgcggg
     5401 cttgcaggaa gtgcgccttg gcttgcagga ctttgtactg gggagtttcc ccagcccgcg
     5461 cgaggcgctc ggtcaagtcg atcgtctgct cggcaatctg gaccatctcc tcaaagactt
     5521 tgacccgctc ctccttgctc tggcagaggt ccagtttggc gctcgccaac gactgcctgg
     5581 cctccaaaac ttcactcagg ctcgtggttc ccactcgata gcccgccagc gtcatttcgt
     5641 gaagctgcgt aacgatggcc agtcgctcct tggccagcgc gaggatttgc tcgttggcct
     5701 tgaccggatc ggcggcaatg gcgggtaccg cacccgctac cgccagtgcg cagacgacac
     5761 ccaaattggc acgcatggga tttctccgca gacggctggt tctattagtg tcggccactt
     5821 ctgggcgcta tccattgcga agcgccgcta gctgccgaat gcaacaggga tttcttcgat
     5881 tggccgccaa ccagcggcga atgcgcaact tgcgccagtg ccacacacga gaggctgcaa
     5941 cgcgattgcg ttagcagcgc agacagaccg acgccagatc ggccagcggt tcatgagagt
     6001 ccgctacggg ccgcgcttcg accgaagacg ggcccgccaa ttccatcgcc acggaaaccg
     6061 gcaggagccg gacgactagc aattggccgc tgggcgttgg ctggctgcgt cgcacctgct
     6121 gactttccca gctcagcgcc tcatgccggc aatcgcaagg cagggggctg gcagcgtcag
     6181 aatcgcactg ttttgccgat tcacgcttgc aacagccgcg gtgctcatga cgcgccggct
     6241 ggcaggcggt gcacgccgca cgcccgccat caatcgagac ggcgccatcc gccgacgtgc
     6301 agacatagag cggaccggcc gctacctgcg tcaccgcgag catcaggcat agtgcagtcc
     6361 gcaacatcga gtcctcgact gcctcacttc tatcacgccc tgcggctgtg gacaacagac
     6421 aattatacca cttcgccgtc gcggggctgg cggccgcagc tatttgggca gcttcggccc
     6481 atcggtccgg taggcgcccc caccgccctg atagcggcgg ccgtgcgacc agctgagatc
     6541 gatccacttg ttcatggccc caaaatagtc ccagccgaac gtgccttcga cgagactcgg
     6601 gctgtcgcca aagagtggca cgccgccgcc gatccagtag ccgccgtagt ggcgcgtgtt
     6661 ggaagggatc gcgtgatggc ggatgagcat gggccagccc gcccggcagt ggtccgcggc
     6721 gcggtggccg tacggatcgc cggccagcgc cagcagcagg gcctgctgtt gctgcgtgcc
     6781 cagctcctca tttgtcaggg gcggcacttc gtcggcacgc agccggagcg ctggcatcat
     6841 cgagatcaca atcacggcaa tgccaaagca cagccgcggg cgatagcgca tcctgcgcat
     6901 gacttagagc ttggggttgc ggttgaactt ttggaaaggc aggcgaacgt tgcgatagaa
     6961 cgtcatgtcg gcccgcaggt gttgcggcgg agcgtagctg tactgggcgt aagacgtgac
     7021 cacgtcgtcc tgatcgacgc cgtcgccgct cacgccgagg ccgccggcca agcccgagct
     7081 gacatacagc ggcgtgctgc ccgggaagaa cacgatgccg ttctgattcg ccaggtcact
     7141 ggggtcgtgg aagttgcggc tggcgtggaa ggcgtcaaaa cccatcacgc tgatgaacca
     7201 cgaggccggc gtgggattgg cctggctcgc gttctcggcc gttttgcggt tgatgcccgg
     7261 atcggtgagg atcgagaaca ccggcggcag cgaaccatcg actccggcgg gatagcgcgg
     7321 ctcagccagg aagcggaacg tgcggttggt gaaggccagt ccggtcaggg gcgaatagcg
     7381 ggccgcgttc ttgctgcctc tatacagatc gggaaacccc agtacgccgt tgtcgttgcg
     7441 gtatttcagt tttcgcagct gcgcgaccgt cgtcgcaccg cgggcgatga gcagatcgtc
     7501 atcggccatg tccacggtct gcaaggccgc ggggtcggcg tagtaggccg tgttgcgggc
     7561 tttggcaatc gccacgtcaa tcgagaagaa cgtcgcgtcg ggcatgcgat agaggcccag
     7621 cacgttgccc gccgtatcgg cgaccgcgat caccatcttg gtcggcacgc cgggcgtcgg
     7681 cggactcttc gtcacgttca ggcggatcgc cgcccggatc tggttggccc ccgtgatcgc
     7741 gttagtgacg atggcctcca catcggcctg cttgatctgg ttcggcccca ccagcggaga
     7801 atcgtgcggc agcaccagcc aaccctcggc cagcggctta ccatcctgtg cgagattgcc
     7861 catcgaatcg atctgccgat tctcacccgt ggtcgtggag ccgacgccca gcgcctggcc
     7921 gcgccggatc acggtgtcga tgccgggcgt tttgaccttg cgagtcgggt tggggccgaa
     7981 cacttcgagc tgaatgccga ccagatcgat gcgcccgaag ggcagcacgt aaccgacagg
     8041 cggcgacagg ccgccaatcg cgccgatcgg ccgcacgccg gtcacaccgc cggcggctgc
     8101 gatggcgacg tattcggctt caagggcttt gggtgcgttg agccgctgcg ccgacgtctg
     8161 cttgatgttt ggaacgaagc cttgctcatg cgtggcgtag ccgtcggggc cgggaaagaa
     8221 tacgccgatg ccgccgacga gcgtgagcgc cccgccgatc gacttgtaga gcggaatgcc
     8281 gccgggcaga gtggccaggc cacggccgat ggcgtgcggc acaaggtcgc tctgcacgcc
     8341 gtacgattcc ggcgcgggga tctcgacgcc cggcgcgagg aacgcgtcgg gaatgttaaa
     8401 gcggttgtcc agatcgatgt cgtcgagcgt gcccttgatg ccgtccagac cgggaagcgt
     8461 caggccgtcg cggctctgca attcgatccg gaacaggtcc acgggcggcg tgtgggcgat
     8521 gtccgcggga aagtgaccgc cgacaccgat cggcgcgacg acgccggggc cgaacgtgcg
     8581 tgagattggg ttcggattga agaacggatt ctgtcccgga tcgcccggca cgggcaccgt
     8641 cggattcgat tcgacctcgc gctcggtgat cgtcgattgg ctgaggttgc ggatcgtccg
     8701 cgaggtcagc ggggcctggt tgttcgcgaa gaaggccgcc gtgcgggcct tggcaacggc
     8761 gccgtcgatg gcaaagaccc gcgtcagcag atcggggatg gcgtcaagga cgccttgctc
     8821 catccgcacg cccagaattc ggccgccgcg atcgacgatc gcgatgatcg cgtcctgcgt
     8881 gctcgtcgcg ccggccgcac gcgccagcag cgtttcgacc tcggcggccg tgatgtagtt
     8941 caagccggcg tcgccgggtg gaatcggcgg cggaggcggc acgatgggca gcggcggttc
     9001 ggcctccgcc tcatcatcca gcggcaggat ggcggcgggc tccgctctcg ccgacagcga
     9061 tgctcccagt gccgccagga cccaatcggc atcggccggc gtgattgccc cgtcggcgtt
     9121 gacatcggca tagaaatcgg ccggcgctgg attcgtctga ttcaggtgat tgatgacgag
     9181 cagcgcgtcg agcggcgaga gttcgccgct agcatccacg tcgcagggac ttgtggagtt
     9241 gtggtgcggc gcgaagctcg cgaattcgtc cgctgtcagc gatggcgcgt cggccgccag
     9301 cagatgccgc ggctccagcc gctcggccga cagtcgccgc gctggtcggg gcagaagttt
     9361 tcgccgacgc aggcctgggc ggtaaacggc gctcacaatg cacacttggc tggtggagtt
     9421 tccagtctag gcaagctcgc tagcggtcgc cagcgaattg ccggtcgctg gtggcccacc
     9481 atcacgcatc ggccggaaaa atcgggacat ttggctccac cgcttcctgc ggcaaagtcg
     9541 atctgatcga tacagcttgc cagagccgca ttgcgcgcgt gcggcggcag gtgcgttgag
     9601 aatcgccgtg atgttgctgg ggggagaacg acgacttggc ccgcgtatcg cagcggcgct
     9661 tgctgctgcg ctggttctgg ccgcgggtct tcacggccag gaaccagtac cagcgccccc
     9721 ttcttccgac ccgactctgc caggagtcct gcgcgagccg tcgctgaatc gagatcagcc
     9781 ggactttgtg ccgctgccga gcttcacggc tcaggatgaa tactttccgc cgctgctgtg
     9841 gcccgtcgat ccgccgctgg gatacacagg gccgtcgggc attgtgccgc gcgaggcaca
     9901 aacgagcagc cactttgtgc ccatggaaga ccgctggcga atcggctttc cggagtggga
     9961 tcggtatggc tggggacatc cgctgctgga ggagtatccc tctgtgccgg gagcgtggta
    10021 cgacccctat catcaaaacg tgctgaaggg gga
//
LOCUS       DASRJB010000739         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2180916, whole genome shotgun sequence.
ACCESSION   DASRJB010000739 DASRJB010000000
VERSION     DASRJB010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180916"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1220
                     /locus_tag="VFB80_19455"
     CDS             156..1220
                     /locus_tag="VFB80_19455"
                     /inference="COORDINATES: protein motif:HMM:NF042558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6666 family protein"
                     /protein_id="HZN36020.1"
                     /translation="MNRNAEPLPSVVGTPMSPEGLPVPPTGSYYPEGDMTNVLPGFGG
                     GHCGCGLLNCGGDCCQPDCCGQWNSCGPVSPCCLLPCVPVNGLEFFAGVQGFTGPLNR
                     GGSGSFGFHEGFNFGVPTCGCLAWQIGANTTQCNFEGNFLTDDSRNQVFLTGGLYRRV
                     DWGIQGGLVVDYLHDEWDYEADLLQLRGEISWRYCCIHEIGFWFTAGVNDADNLTLRL
                     PVQGSTPGIDIVTGQGTVEVNDLYAFFYRRQFACGGEGRAFGGFTANDQGLVGADLLL
                     PINHCWSARAGFLYVTPDGNETENRPDFIEESWNVAISLVWTPCPRQPCVPNYCRPLF
                     NVADNGSFLTRLIPAGLINE"
     gene            complement(1320..2594)
                     /locus_tag="VFB80_19460"
     CDS             complement(1320..2594)
                     /locus_tag="VFB80_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36021.1"
                     /translation="MCLTAIGFCCATPSFSADWPQWRGPEGTGVSTDQGVPIVWHEQR
                     SIVWKCPLPPWGASTPAIWGDAIFVTSHSAENKLLLLKLDRKEGRIAWTQEVGSGEAP
                     RQAPKRTTQKFHELHNLASPSPVTDGKVVVAHFGNGDLAAYDFAGQQLWKRNLQDDHG
                     VYSIWYGHANSPVIAGDVVISVCMQDSLAGLVERPLESYLVAHDLRSGAVRWKLTRAT
                     MADAEQCDAYTTPLLCQLNGVQQLVVMGGNQLDSYDPASGRRLWFLPDLVGGRTVTGP
                     TISEGVIYATRGLRGALFAVRPRPAGELTSRDILWSTTEGTPDTCCPVVASGLVFTVS
                     DDGIARCYLTTNGNLKWKERLKGKYKASPVAASGRIFFLNTEGLATVISASPRFDKLV
                     ENQLDDETIASPAISNGQIFLRGRKSLYCIGR"
BASE COUNT          478 a          961 c          926 g          508 t
ORIGIN      
        1 cggccgaggc gctgccagtc gaaagtccac tgcgtagccc gccgtcagaa acactgcgca
       61 gcccgccgtc agaaacactg cgcacctcac ggctgatgcg gcggcccaag tcgctgtcgc
      121 gtcccgcgcc ggcaatgacg gcgagcgagt cggttttgaa tcgcaacgct gagccgctgc
      181 cgtcggtggt tggcacgcca atgtcgccgg aaggcttgcc cgtgccgccg accggaagct
      241 attaccccga aggcgacatg acgaacgtcc tgcccggatt cggcggcgga cactgtggat
      301 gtggcctgct taactgcggc ggcgattgct gccagcccga ctgctgcggg cagtggaaca
      361 gctgcggccc cgtgtcgcct tgctgcctgc tgccgtgcgt gccggtcaac gggctggagt
      421 tcttcgccgg cgtccagggc ttcaccggac cgctcaatcg cggcggaagc ggcagcttcg
      481 gctttcacga aggcttcaat ttcggcgtgc ctacttgcgg ctgcctggcc tggcaaatcg
      541 gcgccaatac gacgcagtgc aatttcgaag gcaacttcct tacggacgat tcgcggaacc
      601 aggtcttcct caccggcggc ctgtaccgcc gcgtcgattg gggcatccag ggcggcctgg
      661 tcgtcgatta cctccacgac gaatgggact acgaggccga tctgcttcag ctccgcggcg
      721 agatcagctg gcggtactgc tgcatccacg agatcggctt ctggttcacc gcgggcgtga
      781 acgacgccga caacctgacg ctgcggctgc ccgtgcaagg cagcactccc gggatcgaca
      841 tcgtcaccgg gcagggaacg gtggaagtca acgacctgta tgcgttcttc tatcggcgtc
      901 aattcgcctg cgggggcgag ggccgggcct tcggcggctt cacggccaac gaccaggggc
      961 tggtcggcgc ggacctgctg ctgccgatca atcactgctg gagcgcccgg gcgggcttcc
     1021 tctacgtaac gcccgacggg aacgagacgg agaaccgccc ggacttcatc gaggaaagct
     1081 ggaacgtcgc catctcgctc gtctggactc cttgcccgcg gcagccctgc gtgcctaact
     1141 actgccggcc gctgttcaac gtcgccgaca acggctcctt cctcacccgg ctgatcccgg
     1201 cgggcctgat caacgagtaa cgcttcgaat cagttgctcg ttttaacccg cagccggagg
     1261 cgcaggcgtt tcgcggtccc gccgtcgcct ccggctccag attcgacgat gtagagccgt
     1321 caccgtccga tgcagtagag cgacttgcgg ccgcggagga agatctgtcc gttcgaaatc
     1381 gcgggcgagg caatggtctc gtcgtcgagc tggttctcga ccaacttgtc gaagcgcggc
     1441 gaggcggaaa tgacggtcgc caggccctcg gtgttcagga agaagatgcg gccgctcgcg
     1501 gcgacgggtg aggccttgta ttttcctttc agccgctctt tccacttgag gttgccgttg
     1561 gttgtcaggt agcagcgggc gatgccgtcg tcgctgacgg tgaacacgag gccgctggcg
     1621 acgaccgggc agcaggtgtc gggcgtgcct tcggtcgtgc tccacaggat gtcgcgcgag
     1681 gtcaattcgc ccgcgggccg cggccgcacc gcaaacagcg ccccgcgcag gccgcgggtg
     1741 gcgtagatca cgccctcgct aatcgtcggt ccagtcaccg ttcgcccgcc gacgaggtcg
     1801 ggcaggaacc agagccgccg gccgctggcc ggatcgtagc tatcgagctg attgccgccc
     1861 atgacgacga gctgctgcac gccgttcagc tgacatagta gaggcgtggt ataggcgtcg
     1921 cactgctcgg cgtcggccat ggtcgcccgc gtcaacttcc agcgcaccgc gccgctccgc
     1981 aggtcgtgag ccacgaggta gctctccagc ggcctctcca ccagccccgc gagcgaatcc
     2041 tgcatacaga ccgagatcac gacgtcgccg gcgatgaccg ggctgttggc gtggccgtac
     2101 cagatcgaat agacgccgtg atcgtcctgc aagttccgct tccagagctg ctggccggcg
     2161 aagtcgtacg ccgccaggtc gccgttgcca aagtgagcga cgacgacctt cccgtccgtc
     2221 acgggcgacg ggctggccag gttgtgcagc tcgtggaatt tctgggtcgt ccgtttgggc
     2281 gcctgccgcg gcgcttcgcc ggaaccaact tcctgggtcc atgcaatgcg gccttccttg
     2341 cgatccagct tcaggagcag cagcttgttc tccgccgagt gcgaggtgac gaaaatcgcg
     2401 tcgccccaga tcgccggtgt actcgcgccc cacgggggca gcgggcactt ccagacgatg
     2461 ctccgctgct cgtgccacac aatcggcacg ccctggtcgg tgctgacgcc cgttccctcc
     2521 ggcccgcgcc actgcggcca gtcggcggaa aacgacggag ttgcgcagca gaagccgatc
     2581 gctgtgagac acaaaggcag tacagcctga cgcaacggta cctcgtactc cgtactgact
     2641 actgagtacc gagtacgcag tgcggcgtgc ttcatgaaca cagctcctgg aatcatgggg
     2701 ccagcctatg ccactactcg cttgtgcttc tcccccgcga cttcctgcac gtaccgcggc
     2761 accgcgtagc cgggcaagga ccgccgcatt tcctggacga gcgcaagccc gcggctgatc
     2821 ggcacttcaa aatgagccgc cccgcggacg cggtcgagct gatgcaggta ata
//
LOCUS       DASRJB010000740         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2182127, whole genome shotgun sequence.
ACCESSION   DASRJB010000740 DASRJB010000000
VERSION     DASRJB010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182127"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFB80_19465"
     CDS             complement(<1..979)
                     /locus_tag="VFB80_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZN36022.1"
                     /translation="MIPLTRNSAVTQLDVALAERKLTPSAAENIRRWLSEPRYGEYAP
                     QVAEHIAAGKWKELDDVFWTVIPFGTGGRRGRMYPIGCNAINDRTIGESAQGLAEYVK
                     DVHVPSSEFKVHSSETANSELRTRNSELRCAIAYDTRHNSRRFAELCASVMVANGFRV
                     YFLDGFRSTPELSFLVRHKQCACGIMVTASHNPPSDNAVKVYWSTGGQVIPPHDQGIV
                     ERVMNVQEIRRADFGPAVAEGQIVFCQAEIDAAFIQSVLGQRCPGPRALKIVYSPLHG
                     VGEFAAVPALEADGFAEIEIFGPHREPSGDFPNVPGHVSNPENAAVFDAI"
     gene            1082..3355
                     /locus_tag="VFB80_19470"
     CDS             1082..3355
                     /locus_tag="VFB80_19470"
                     /inference="COORDINATES: protein motif:HMM:NF012771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36023.1"
                     /translation="MERLPLLTPDSDRRAFKRPLRVWASDEDFGPACRIGRRVLDDGL
                     FTSLEEHFPEVKVTSVHRAERRIAADNEFLPPGQRERLDERGVIRAGSVIRAGDVLVS
                     IVETPSSGKSRDDSYCAPPECDGAAVRQVTHRRPDEMGHSPPQGLQALVSLELVAHRE
                     LEVGDVLAIGEEPIVVAGVLEQPPLDDSGQPADLVLSAAVARRLHLAPGAHCEQPVSK
                     ANARAAESLEVRATGGYSLITMQPLWLGANPCQTLTLPQVAWLAERELTGILEELTGL
                     KSDNLVARAELRRAVERGALDTFDAAVGGASEMLAVVALLMRSLGLNVELRRGDRCAS
                     LALAPATSQELISMSSGLIQKPETLNYRTSDYEPGGIFCPQVFGESLPTRRRRFAHVS
                     LPAPIVSPLWRAGKPSLLEQLLGLTSKQIEGILACQTNVVCQGDEVRLEPRQIDASAR
                     PSASPEPTAETGGVAIRTLLARLPPERLPPALRGRAEALAPDIVLVLPPDLRPIVLLD
                     SGNFATSDLNDLLRRLINRRNRLVKLAELKAPQVILDNEQRMLQGCVDELHANCLLPR
                     KQAALGSENQPLKCTLGFLAGFLLDEDSKRVEWAGRARAVIDAGRPEGRIAVPRSMFE
                     GLQLTADRPVLLTTPEGAAWVAGLPQMHEARVIFVSPVAAADLDLPRDQPAECILHRP
                     IGPAARTEAQRLLEHGPPRRRRAIGGSWAQASDPEQLAVQLAAAAASGEAIHLDSPQH
                     LLLAGAGGIDLELSDES"
     gene            complement(3763..4041)
                     /locus_tag="VFB80_19475"
     CDS             complement(3763..4041)
                     /locus_tag="VFB80_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36024.1"
                     /translation="MATAKINTDAGIWQRVLEFDSPPSPAAASALLKLQFPEDDRRRM
                     KQLTAKARAGQLSRAEKSEAEAYERLGCLLDILHSQARRVLKQRRTAS"
     gene            complement(4094..6019)
                     /gene="hemE"
                     /locus_tag="VFB80_19480"
     CDS             complement(4094..6019)
                     /gene="hemE"
                     /locus_tag="VFB80_19480"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZN36025.1"
                     /translation="MTAEKGDFAGLRVAALESRNGPEMARLIEKFGGRAFVSPSMREV
                     PIEQNREAIDFAHRLLTGEIDVMIFLTGVGFRHLLAAVERHVERQRYLNALADITTIC
                     RGPKPVAAMAEVGLKPTHKVPEPNTWRELLATIDGGIPIAAHTVGLQEYGVTNHSLIA
                     GLEARGARVVNVRVYQWELPADTAPLEQNIRAIAAGERDAIVFTSAHQVANLLRMAQQ
                     LSLEQPLRGALTRMAVASVGPTTSEMLRDHELPVDIEPEHPKMGPLIAAAAEQAAEVL
                     RRKRQTSIAVVAPAANPLDSNAPWFNSPFLKACRREPTEVTPVWLMRQAGRYMEEYRQ
                     VRGTTSFLDLCKNPQLCSEIMCTAVRRLGVDAAIIFSDLLPILEPMGLELDFAKGEGP
                     VIYNPVRAPADVDRVLELEELSALDFVFETVRRTRADLPSQLPLIGFAGAPFTLASYT
                     IEGGSSRSYLHTKTLMYRDEGAWRTLMERLSRSITRYLNAQIAAGAQCVQLFDSWVGC
                     LGPDDYRRYVLPFVKSIIGGITPGVPVINFATGNPALLPLLSEAGGDVIGIDWRVRLD
                     GAWQAVGEDRAVQGNLDPLVLLAEPAEIRRRAKEVLKQAAGRPGHIFNLGHGVVQQTP
                     VENAIALVDAVHELSRR"
     gene            complement(6161..6541)
                     /locus_tag="VFB80_19485"
     CDS             complement(6161..6541)
                     /locus_tag="VFB80_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36026.1"
                     /translation="MFEQFTESDRAVLAQLLALQVPKKEIASRLSKDRSSMDLELARN
                     SGPLGYVPIEAQQRAMARRRLPRPQERRRKNARLCITQLPASFVPFVPSVQSAGPHAE
                     RDHWSRLWPRECLTCIKLHNGRRR"
     gene            6713..7459
                     /locus_tag="VFB80_19490"
     CDS             6713..7459
                     /locus_tag="VFB80_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36027.1"
                     /translation="MTIEREGKFFVDHDLKRRLSIGMDSRKLSVCVAEAIRRRAYGVF
                     GNPEYHFKETNLDFLKDLPRIQKIWFFDINLKNVDGIYPLKQLKEFGVHPKRPPIKFE
                     LLPTLEFAEWQYKDGDSGMATLKSLKDLGLAHYNPKSASFEGLELPLNLVELGIAWVN
                     PRTLAGLPKLIHLKKLVIGRCRNLETLKDLPIIAPSLQRLEIESCGRLADVEQVIRRL
                     PKLRKAFLDEKVLLDLPERPATAVRHRVAH"
BASE COUNT         1364 a         2497 c         2375 g         1410 t
ORIGIN      
        1 tgatcgcatc aaagacggct gcgttctccg gattcgagac gtgtcccggc acgttgggaa
       61 aatcgccgct gggctcgcgg tgcgggccaa agatctcgat ttcggcaaag ccgtcggcct
      121 caagcgccgg cacggccgca aactcgccga cgccgtgcag cggcgaatag acgatcttca
      181 gcgcccgcgg ccccgggcag cgctgaccga gcacgctctg gataaacgcc gcatcgatct
      241 ccgcctggca aaaaacgatc tggccttccg cgaccgccgg cccgaagtcg gcgcggcgga
      301 tctcctgcac gttcatcacc cgttcgacga tcccctggtc gtgcggcgga atcacctgcc
      361 cgcccgtgga ccagtagacc ttcaccgcgt tgtcgctcgg cgggttgtgg ctggccgtca
      421 ccatgattcc gcacgcgcac tgcttgtgcc gcaccagaaa cgacagctcg ggcgtgctgc
      481 gaaatccgtc caggaagtac acccgaaagc cattggccac catcaccgac gcgcacagct
      541 cggcaaaccg gcggctgttg tggcgcgtgt cgtaggcaat cgcacagcga agttccgagt
      601 tccgagttct gagttccgag ttggctgttt ctgaactgtg aactttgaac tcggaactcg
      661 gaacgtgcac gtccttgacg tactccgcca gcccctgcgc actctcgccg atcgtccggt
      721 cgttgatggc gttgcagccg atggggtaca tcctgcctcg cctgccgccg gtgccgaacg
      781 gaatcaccgt ccagaagaca tcgtccagct ccttccactt ccccgcggcg atgtgctcgg
      841 ccacctgcgg cgcgtactcc ccataccgcg gctctgagag ccagcggcga atattctccg
      901 cggccgacgg tgtgagcttt ctctcggcaa gcgcgacgtc gagctgcgta acggctgaat
      961 ttcgggtcag gggaatcatt gatcctagac ggccagtgcg agaatccgta aaaccctgaa
     1021 tctgacgcag tttaggaaac cgcggcccgc ttgggcagac cgcggcctgc tcgcacacgc
     1081 tatggaacga cttccgcttc tgacgccaga ttccgatcgc cgcgccttca agcggccgct
     1141 gcgcgtctgg gccagcgacg aggacttcgg tccggcgtgc cgcatcggcc gccgggtgct
     1201 cgacgatggt ctgtttacat cgctggagga gcactttccc gaagtgaaag tcaccagcgt
     1261 acatcgcgcc gagcggcgga tcgccgcgga caacgagttc ctgccgcccg gccagcggga
     1321 gcggctcgac gagcggggag tgattcgagc cggaagcgtc atccgcgcgg gcgacgtgct
     1381 cgtgagcatc gtcgagacgc cgtccagcgg taagtcccgc gacgattcgt actgcgcgcc
     1441 gccggagtgc gacggcgccg ccgtgcggca ggtcacgcac cgtaggccgg acgaaatggg
     1501 ccacagcccc ccgcaaggct tgcaagccct ggtttctctg gagctggtcg cccatcgcga
     1561 gctggaggtc ggcgacgtgc tggcgatcgg cgaagagccg atcgtcgtgg ccggagtgct
     1621 cgagcagccg ccgctggatg attccggcca gcccgccgac ctggtcctct ccgccgcggt
     1681 cgcccggcgg ttgcacctcg cgcctggggc gcactgcgag cagccggtca gcaaggccaa
     1741 tgcgcgggcc gccgaatctc tggaagtccg tgccaccggc ggatattcgc tgatcacgat
     1801 gcagcctctc tggctcggtg ccaatccctg ccagacgctg acgctgccgc aggtcgcgtg
     1861 gctggcggag cgcgaactaa ccggcattct ggaagagctg acgggcctca agtccgacaa
     1921 cctggtcgcc cgggcggagc tgcgaagagc ggtggagcgc ggggcgctcg acacctttga
     1981 cgctgcggta ggcggggcgt cggaaatgtt ggccgtggtg gcgctgctca tgcgttcgct
     2041 cggcctgaac gtcgagctgc gccgtggcga ccgctgcgcg tcgctcgccc tggcgccggc
     2101 tacgagtcag gagctgatct cgatgtcgtc cgggctgatc cagaagcctg agacgctcaa
     2161 ctatcgcacc tccgattacg agcccggcgg aatcttctgc ccccaggtgt tcggcgagtc
     2221 gctcccgacg cggcggcggc gctttgctca cgtttcgctc cccgcgccga tcgtctcgcc
     2281 gctctggcgt gcgggaaagc cgtcgctgct cgagcagctg ctcggcctga cgtccaagca
     2341 aatcgaagga atcctcgctt gtcaaacgaa tgtcgtctgt cagggggatg aagtccgact
     2401 cgaaccgcgc cagatcgacg cttcggcacg accttccgcc agcccagagc cgacagccga
     2461 aaccggcggc gtcgctatcc gcacgttgct ggcccgcctg ccgccagagc ggctcccgcc
     2521 ggcgctgcgc ggccgtgccg aggcactagc acccgatatt gtgctcgtcc tgccgccgga
     2581 cctgcgcccg atcgtcttgc tcgacagcgg caatttcgcc acgtcggacc tcaacgacct
     2641 gctccgccgg ctaatcaacc gccgcaatcg cctggtcaaa cttgccgagc tgaaagcccc
     2701 gcaggtgatc ctcgacaacg agcagcggat gctgcagggc tgcgtggacg agctgcacgc
     2761 caactgcctg ctgccgagaa agcaagccgc actcggttcc gaaaaccagc cgctgaagtg
     2821 tactctcggc tttctcgccg gatttctgct cgacgaagac tccaagcgcg tggagtgggc
     2881 cggccgggca cgcgccgtca tcgacgcggg ccggccggaa ggccgcatcg ccgtgccccg
     2941 ttcgatgttc gagggcttgc agctgaccgc ggaccggccg gtgcttctca cgacgcccga
     3001 gggcgcggcc tgggtcgccg gcctgccgca aatgcacgag gcccgagtca tcttcgtctc
     3061 tcccgtagcc gcggcagatc tggatttgcc gcgcgatcag ccagccgagt gcatcctgca
     3121 ccggccgatc ggtcctgctg cccgcaccga ggcccagcgg ctgctggagc atggccctcc
     3181 gcgccgccgg cgcgcgatcg gcggttcctg ggcccaggca tccgatccag agcagctcgc
     3241 ggtgcagctg gcggccgcgg cagcctccgg tgaagccatc catctcgact cgccgcaaca
     3301 cttgctgctc gccggcgcag gcggtatcga tctggagcta agcgacgagt cttagtcgcg
     3361 gtgaaaaacg ccttcggcaa tcagcgcagc ccgctgggcg atgcggtgag gagcgttgat
     3421 ttgcaggacc gcaattgttg cccgccctat cggcgtgcgt ccgaccagca cggcgccctt
     3481 ccaacgaaag tggcgctccc atttatgccg tcgcgggtga aagagccatg ctctccgccc
     3541 tgtacgtgga tcgatgcccg ccagattagg tcccttatga tgattgtcgg cgaagcatgc
     3601 ccacgccagg ttgcctggaa tcgaccggcc accgtgctgc tcggcaacga tgtggtcaat
     3661 ctcgaacccg atcgaggagt aagcttccgg caggcagcaa tactcgcacc ggcccctagc
     3721 ccgctggcga acgagccggg ttagctgttg actgacggcc attcaggatg ccgtccgacg
     3781 ttgcttgagc actcggcggg cttgcgaatg cagaatgtca agcaggcagc ccaaacgctc
     3841 gtacgcttca gcttcggatt tctcggcgcg cgacaactgt cccgcccgag cctttgcggt
     3901 gagctgcttc atccgccgac ggtcgtcttc agggaactgc aacttgagca gcgcgctggc
     3961 cgcggccggc gaggggggcg aatcgaactc gagcacgcgt tgccagatcc ccgcatcggt
     4021 gttgatcttc gctgtagcca tctcccgctt cctctgtttg caattctagc actcgccacg
     4081 cgcgactgtt atctcaccgc cggctgagct cgtgcacggc atcgacgagc gcgatcgcgt
     4141 tctcgaccgg cgtctgctgc acgacgccgt ggccgaggtt gaagatgtgg ccgggccggc
     4201 cggcggcctg cttgaggact tcctttgcgc ggcggcggat ttcggccggc tcggccagca
     4261 gcacgagcgg atcgagattg ccctgcaccg cacggtcctc gccgaccgct tgccaggcgc
     4321 catccagccg cacccgccag tcgatgccga tcacgtcgcc gccggcttcc gagagcagcg
     4381 gcaagagcgc ggggttgccc gtcgcaaagt tgatcaccgg cacgccgggc gtgatgccac
     4441 cgatgatcga cttcacgaac ggcagcacgt agcggcgata gtcgtccggg ccgaggcagc
     4501 caacccacga atcgaacaat tgcacgcact gcgcgccggc ggcgatctga gcgttcaggt
     4561 aacgcgtgat cgagcgcgac agccgctcca tcagcgtccg ccaggccccc tcgtcgcggt
     4621 acatcagcgt cttggtatgg aggtagctgc ggctgctgcc cccttcgatc gtgtagctgg
     4681 ccagcgtgaa cggcgctccg gcaaagccga tcagcggcag ttggctcggc aagtcggccc
     4741 gcgtgcggcg gacggtctcg aagacgaagt cgagcgcgct cagttcttcc aattccagca
     4801 cgcgatcgac gtcggccggc gcacgcacgg ggttatagat gacgggccct tcgcccttgg
     4861 cgaaatcgag ctccagcccc atcggctcca ggatcggcag cagatccgag aaaatgatcg
     4921 ccgcatcgac acccagccgg cgcacggcgg tgcacatgat ctcgctgcag agctgcggat
     4981 tcttgcagag gtcgaggaag ctcgtcgtgc cgcggacttg ccgatattcc tccatgtagc
     5041 ggccggcctg ccgcatcagc cagaccggcg ttacctccgt cggctcccgg cggcaggcct
     5101 tcaggaacgg gctgttgaac cagggggcgt ttgaatcgag ggggttggca gcgggcgcaa
     5161 cgaccgcgat ggaagtttgc cgcttccgcc gaaggacttc cgcggcctgc tcggcagcgg
     5221 cagcaatcag cggccccatc ttcggatgct ccggttcaat atcgacgggc aattcgtggt
     5281 cgcgcagcat ctcgctcgtt gttggcccga ccgaagccac ggccattcgc gtcagtgcgc
     5341 cgcgcagcgg ctgctcgagg ctcagctgct gtgccatccg cagcaggttc gcgacctggt
     5401 gggcgctggt gaatacgatc gcgtcccgct cgcccgccgc gatcgcccga atgttctgtt
     5461 ccagcggagc ggtatcggcc ggcaattccc attgatacac ccgcacgttc acgacccgcg
     5521 ccccgcgggc ttccaggccg gcgatgagac tgtggttggt gacgccatat tcctgcaggc
     5581 cgaccgtgtg ggcggcaatc ggaatgccgc cgtcgatcgt cgccaagagt tcccgccagg
     5641 tgttcggctc cggcactttg tgcgtcggct tcagacccac ctcggccatc gccgcaaccg
     5701 gcttgggacc gcggcagatc gtcgtgatgt ccgccagggc gttaaggtac cgctgccgct
     5761 cgacgtgccg ctcgaccgcc gccaacagat gccggaagcc aacccccgtc aggaagatca
     5821 tcacgtcgat ctcgccggtc agcaggcggt gggcgaagtc gatcgcctcg cggttctgct
     5881 cgatcggcac ttcgcgcatg ctcggactga caaacgcccg cccgccgaat ttctcgatca
     5941 gccgggccat ctcgggccca ttgcggctct ccagcgcggc cacgcgcagg ccggcaaaat
     6001 ctcctttttc tgcggtcatg ggttcatgct acttggccag ggaaacccgc gacaggtagc
     6061 cgccgcgagg cctgcgggca gctcgtgcag ttgcataatt cagaaaactt ccggtagtcg
     6121 gtttggatgt aagtggttgt ggcataactg cttacgtcat tcacctgcgg cggccgttat
     6181 gcagctttat gcaagttagg cattcgcgtg gccaaagccg cgaccagtgg tcgcgctctg
     6241 catgtgggcc ggcagattga acagaaggaa cgaagggaac gaaggaagca ggaagctgcg
     6301 ttatgcatag gcgagcattt ttccgccggc gttcctgcgg gcgcggcagc cggcgtcgcg
     6361 ccatcgcccg ttgctgggct tcgatgggaa cgtaaccgag gggcccgcta ttgcgggcca
     6421 actcgagatc catcgaagaa cgatccttgc tgaggcgcga ggcaatttcc tttttcggca
     6481 cctgcagcgc gagcaactgc gctagaaccg cacgatccga ctccgtaaac tgctcgaaca
     6541 tcgctgggac tccgtttgaa tgtccgtcct tcacaaccag acattcagac atcccagcga
     6601 tgttttcacg aacaccaatt ccgcgctgct agcacgaacg cgtgtcgcat ttgaatttgg
     6661 aaaccgcccc ttttttgctc ggttcaaagg cggacgtcga aggagaagat tcgtgacaat
     6721 tgagcgcgag ggcaagttct ttgtcgatca tgacctcaag cggcgactgt cgatcgggat
     6781 ggactcgcgc aaactgtctg tttgtgtcgc agaagctatt cgtcgacgtg cgtatggggt
     6841 gtttggtaac cctgaatatc acttcaagga aacaaatctg gactttttga aggacctgcc
     6901 tcgaattcaa aagatttggt tctttgacat taacctcaag aatgtggacg gcatctatcc
     6961 actgaagcaa cttaaggaat tcggcgtaca cccaaaacga cccccaatca aatttgagct
     7021 cctaccgaca ctggaatttg cagaatggca gtacaaggac ggcgattctg gtatggcgac
     7081 tctcaaatcg cttaaggatc ttgggctcgc gcactacaat ccaaaatccg ccagctttga
     7141 gggcttggag ttgccgctca atctcgtaga actaggtatc gcctgggtga atccgagaac
     7201 gcttgccgga ctgccgaagc taattcacct gaagaagctc gtaattggtc gatgccgcaa
     7261 cctggaaacg ctgaaggacc ttcccattat cgcgccgagt ttgcagcgcc ttgaaataga
     7321 aagctgcggc cgattggcgg acgttgaaca ggtcattcgc agacttccga aacttcgaaa
     7381 agcctttctg gatgagaagg ttctcttgga cttacccgaa cgaccggcaa ccgccgtccg
     7441 acatagagtc gcgcattagc gtcttggtcg ggtgcagcaa cttgccgcga cggaccaatc
     7501 atgttcttcc gaaggcgtcg gaccgccaac ggggatacgt ccaattgtcg gcaacgacgg
     7561 gacttcactg ggacgtaccc cccttggcat ttggttctct cgtccaagtg atgatgagaa
     7621 ccactcgaac ctttctcact ccgaat
//
LOCUS       DASRJB010000741         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_502606, whole genome shotgun sequence.
ACCESSION   DASRJB010000741 DASRJB010000000
VERSION     DASRJB010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502606"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFB80_19495"
     CDS             <1..1143
                     /locus_tag="VFB80_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36028.1"
                     /translation="GLLTPPPPRTTTTGTGGMVLTPIQPKSPASTPAIAPPVAATPPA
                     AAPPAAAPRAVEPAFTADSRAAPTSSNLKVTKGAGILPNEHGQVWREYDIAPYTSRAA
                     GAAKPEQAIIDWILRETGTEVWFSEPLGILSATPSTLRVYHTPEMHDTIRGIVERFVA
                     GDGQPHALSVCLMTVGSPNWRAQAISLLKPVDVKSPGVEAWLLSRENATRLREQLKAR
                     GDFREHSAPQAEIPNGHKQTLARTQPRKYTRGVQLKREYPFYDLIPGQIDEGYSLELS
                     PLLSLDGQTIETAVSCHVDQVEKLVPLAIDLPIGGQTQRVQIQVPQLVSWRLAERFRW
                     PAGEVLLLSCGVVATPAPGSGGPLAFLNPLASGTRADALLLIEHKP"
     gene            1161..1901
                     /locus_tag="VFB80_19500"
     CDS             1161..1901
                     /locus_tag="VFB80_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36029.1"
                     /translation="MRKLGVALALAAALLAAPGCAGWLRPKAGGPLPLSNPLLVPPID
                     REFLWNQTVDALDDYFHIEREDRVRLISGILTEGRIDTFPVTGSTIFEPWRFDSTRGY
                     EKWHATLQSIRRRATVRVIPAEGGYLLDVIVQKELEDLDKPEHATAGGATLRHDGTLV
                     RQEGAPGRYSVTLGWIPIGRDVSLEQRILADLVARLDLHAGVRHAEPGLEEVLSPPGE
                     PIGPQLLPPQSPAGPELLPQPLGRLPAP"
     gene            complement(1902..2444)
                     /locus_tag="VFB80_19505"
     CDS             complement(1902..2444)
                     /locus_tag="VFB80_19505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN36030.1"
                     /translation="MSRTFLSFAGVLVLVPLALAADKPKDGFERERKEGDTTKDSLEG
                     KTPPALKVQDWVNTGGKDLKLADLKGKVVVLDFWGTWCGPCRAAMPHLKELYTKHKDA
                     GLVVIGVHTTNGGEEMAKYVQDEALPWPIALDVDKGTVTAFHVDSYPDYYVIDKAGNL
                     RVADLANKELDRVVEALLKE"
     gene            complement(2963..3196)
                     /locus_tag="VFB80_19510"
     CDS             complement(2963..3196)
                     /locus_tag="VFB80_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36031.1"
                     /translation="MTLRYKLRTLLILLAILPPLLWIGWTTYPAWKAEQSAAAGDGNT
                     ANSCAAGSVIGLPAKPAVNTRPPPDSAPELPRE"
     gene            complement(3193..3525)
                     /locus_tag="VFB80_19515"
     CDS             complement(3193..3525)
                     /locus_tag="VFB80_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36032.1"
                     /translation="MQEALAQYAAFGPLAHELRLARLFVCGYNAGMPKKTRKPTYEDL
                     CDLLLYLDSDDDDGRPVAYEALGLLIQAGAISWQGKKHFDLTECGEQIAKRLRNDNTA
                     QELREVLE"
     gene            complement(3556..4035)
                     /locus_tag="VFB80_19520"
     CDS             complement(3556..4035)
                     /locus_tag="VFB80_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36033.1"
                     /translation="MAESKQEEIKRRDKREAAIHEAGHLTVGLALGVRGSAWVYRSET
                     TDPDNEKLWIGKHEAHGLVTATVAVSGLVAECWVEKPETSPDEIADWIDDGLVSPSPT
                     DKTRFPESYEETLTAIVEAHAILVKHKRFFEWAVDELIKFETITDGMAAEKWRELYA"
     gene            5201..5362
                     /locus_tag="VFB80_19525"
     CDS             5201..5362
                     /locus_tag="VFB80_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36034.1"
                     /translation="MKTAVISPLRFAFLVALVLCPLTFYAVWAFGPFGSLLIMAVPAA
                     FALYFPRPD"
BASE COUNT          984 a         1818 c         1734 g         1082 t
ORIGIN      
        1 ggcctgttga ctccgcctcc gccgcggacg acgacgaccg gcaccggcgg catggtgctg
       61 acgccgattc aacccaagtc gccggcgagc accccggcaa tcgcgccgcc ggtagcggcc
      121 acgccgcccg ccgctgctcc acccgccgcc gctccgcgcg cggtggagcc cgctttcacc
      181 gccgattccc gcgcggcgcc gacgagcagt aacttgaagg tcaccaaggg cgcgggcatc
      241 ctgcccaacg agcacgggca agtctggcgc gaatacgaca tcgcgccgta cacctctcgc
      301 gcggccggcg cggccaagcc cgagcaggcg attatcgact ggattctccg cgagaccggc
      361 accgaagtct ggttcagcga gccgctgggc atcttgagcg ccacgccgag cacgctccgc
      421 gtctatcaca cgcccgagat gcacgacacg attcgcggca tcgtcgagcg gttcgtggcc
      481 ggcgatgggc agccgcatgc cttgagcgtc tgcctgatga cggtcggcag ccccaactgg
      541 cgggcgcagg cgatctcgct gctcaagccg gtggacgtca agtcgcctgg cgtcgaggcc
      601 tggctgctgt cgcgcgaaaa cgccacgcgg ctccgcgagc agctgaaggc ccgcggcgac
      661 tttcgcgagc acagcgcgcc gcaggctgag atccccaatg gccacaagca gacgctggcc
      721 cgcacgcagc cgcggaaata cacgcggggc gtgcagctga agcgcgaata ccccttctac
      781 gacttgatcc ctgggcagat cgacgagggt tactcgctgg agctcagccc gctcttgtcg
      841 ctggacggcc agacgatcga gacggccgtg agctgccacg tcgatcaggt cgagaagctg
      901 gtgccgctgg cgatcgattt gccgatcggc gggcagacgc agcgggtgca gattcaggtg
      961 ccgcagctgg tgagctggcg gctggccgag cggtttcgct ggccggcggg ggaagtgctg
     1021 ctgctctcgt gcggcgtcgt ggcgactccg gcccccggca gcggcggacc gctggcattt
     1081 ttgaatccgc tggctagcgg cacgcgggcc gacgcgctcc tgctgatcga gcacaaacct
     1141 taggtcttcg aggcggcgac atgcggaagc tcggcgtggc actggcgctc gcggcggccc
     1201 tgctcgctgc gcccggctgc gcgggctggc tgcggccgaa agccggcgga ccgctgccgc
     1261 tatcgaatcc gctgctggtg ccgccgatcg atcgcgagtt cctttggaac cagaccgtcg
     1321 atgccctgga cgactacttt cacatcgagc gcgaggaccg cgtgcggctg atctcgggca
     1381 tcctgaccga aggccggatc gacacgtttc ccgtcaccgg ctcgacgatt tttgagccct
     1441 ggcgtttcga ttcgacgcga ggttacgaaa agtggcacgc cacgctgcag tcgatccgcc
     1501 ggcgggcgac ggtccgcgtg attccggcgg aaggcgggta tttgctcgac gtgatcgtgc
     1561 agaaggaact ggaggacctg gacaagccgg agcacgcgac ggccggcggg gcgacgctcc
     1621 gccacgacgg cacgctcgtc cgacaggaag gcgcgccggg ccggtactcg gtcacgctcg
     1681 gctggattcc gatcggccgc gacgtgtcgc tggagcagcg gatcctggcc gacctagtgg
     1741 cgcggctcga tctacatgcc ggcgtgcggc acgccgagcc cggcctcgaa gaagtcctgt
     1801 cgccgcccgg cgaaccgatc gggccgcagc tgctgccccc gcaatcgccc gccggcccgg
     1861 aactattgcc gcagccgctg ggtcgcttac ccgcgccgta gctattcctt cagcagcgct
     1921 tcgacgacgc ggtcgagctc tttgttggcc aggtcggcga cgcgcagatt gccggccttg
     1981 tcgatgacgt aatagtcggg atagctatcg acgtggaagg ccgtgacagt gcccttgtca
     2041 acgtccaggg cgatcggcca gggcagcgcc tcgtcctgaa cgtacttggc catctcttcg
     2101 ccgccattgg tcgtgtggac gccgatcacg accagcccgg cgtccttgtg cttcgtgtac
     2161 aactccttca ggtgcggcat cgcggcgcgg cacggcccgc accaggtgcc ccagaagtcg
     2221 agcactacga ccttgccctt caaatccgcc agcttgaggt ccttgccgcc cgtgttgacc
     2281 caatcctgca ctttcagcgc cggcggcgtt ttgccttcca gcgagtcctt cgtcgtgtcg
     2341 ccctccttgc gctcgcgctc gaagccgtcc ttcggcttgt cggccgccaa tgccagcgga
     2401 accagaacga ggaccccggc gaacgaaagg aaggtgcgcg acatgagatg actcctgaga
     2461 tgaaacgtaa ccccgcggcg tagctgccga agctgcaaca taccgccgcg acacggcgtc
     2521 cggcaatcgc ctcgacacac ttctgaaatc gcgccgccgg ccgtcaacct tttagcccca
     2581 ccggcccgtc cgccgttgcc tgtcccgatg cggaaaagag ggaaaatcgg ggcggggttt
     2641 gcgaacgtca actggcttgc taccatcact tctcagggtg tcgcgacacc ggcgggtaca
     2701 agaactacca ccgactgcga cccacagccg tcgccacaag tcattgatat aaaacaactt
     2761 aggtcaggta gctcagttgg tagagcaatg gactgaaaat aggccgcgaa aatcggcctg
     2821 attgtgcgaa tcgcttggga attgactggc gaaatcgcaa tttctgcccc tgttgggcga
     2881 gccgcggaaa cagtgggaaa actgcggttt gggagcgctt gcgggtgtca agtacaccgg
     2941 ttggccccag cggcagcttg cgttactccc gcggcagttc cggcgcactg tcgggcggcg
     3001 gccgcgtatt gacggctggt ttggcgggga gaccaatgac acttcccgcg gcgcaactgt
     3061 tggcggtgtt gccatcacca gccgcggcgg attgctccgc cttccacgcc ggatacgtcg
     3121 tccagccgat ccacaacaac ggcggcagga tggccagcag gatcaggagc gtgcgaagtt
     3181 tgtagcggag ggtcattcca gcacttcccg caattcctgc gcggtgttgt cgttccgcag
     3241 tctcttggca atttgctcgc cgcactcggt gaggtcaaag tgttttttgc cctgccacga
     3301 gatcgcgccg gcttggatca gcagccccag cgcttcatac gcgactggcc taccatcatc
     3361 atcgtcgctg tccaaataga gcagcaggtc gcacaggtct tcataggtcg gcttgcgcgt
     3421 ctttttcggc atgcccgcat tgtacccgca cacgaaaagc cgcgccagtc gaagctcgtg
     3481 ggcgagcggt ccgaatgcgg cgtactgtgc cagtgcctct tgcacaacgg tctcgcacgc
     3541 cgcttcgtct agtagttacg cgtacaactc gcgccatttc tcggcggcca tgccatcggt
     3601 gatcgtttca aacttgatca actcgtccac ggcccactca aagaaacgtt tgtgcttcac
     3661 tagaatcgcg tgtgcctcga caatggccgt gagcgtctct tcgtaggatt ctggaaatct
     3721 tgttttgtct gtgggcgaag gagagacgag cccgtcgtcg atccagtctg caatctcatc
     3781 cgggcttgtc tctggttttt ccacccaaca ctcagcaact aggccagaga cggcgacggt
     3841 cgcggtaacg agcccatgtg cctcgtgctt acctatccac agtttttcgt tgtcaggatc
     3901 ggtagtttcg cttctgtaaa cccaagcgct tccgcgtaca ccgagcgcga gccccacagt
     3961 aagatgcccg gcctcgtgaa tagcggcttc gcgtttatcg cggcgcttga tttcctcctg
     4021 cttgctttct gccatcgtcg tcgctccaag aaacttgcct gccgcgactc ggcgacgggc
     4081 cgcgttgatg gtaatcgctc aacgatcgtc gcgagccaca gcaaccgcag ccacggcatc
     4141 agccgcagcg gtcgggctgt cggctgagtc tcgaagaaat tagcggcgct cgcggatcgt
     4201 gggtcgtgtc ctactcgtca aactgaacct caactgccca ctgcctggcg aggaatatag
     4261 cggcatcata tagctcgtct cgcgtaactg cctgcctggc tgcgtccagc agcgcagtgt
     4321 attggtccgc ggtcaacctg ggctgaaagg ctagaaatgc cgcgccatct cgcgtgagca
     4381 agcgaaggcg gggtttccag ttcgatgtgc agttgggctg gcattttgag cctccgttgc
     4441 ttgactggcg gcaagaaaca aaaaagccga cgtgccgatt ctgcccgaag gcatttcggt
     4501 acgtcggctt actgattaac gagccacccg gcgctgccgg actgctcttt atctagtcat
     4561 ccgaactctt ggcgcgctgt cgattttagc aaggcattgg gcgcgtgcaa gcaccggcgg
     4621 cccagcgcca agacgatcag gctggccctc tccgccagct cgtcgaaaaa ctcggtgcac
     4681 cacttcgttt gcgtcgtttt cgctcaattc cccgtctgac tttagccggc taacgaaatc
     4741 gagcgcccgc gagggtgaat cgaaccgggt ggcgtaggcc acgcatttct ttacggcggc
     4801 ggtgattctg tcggctgatt cgctcatggc agccaagggc tgtgcaaact cggaacgcac
     4861 ggccggcaaa tacgccaatc agcagaccaa tcagcacgta tggcaggcgg ggaaacatcg
     4921 aaattgattg taccgccgca cgaaaaagcc ccgccagccg aagcggaatg ggttgatcat
     4981 tgccagctgt cgcgcctcgg cgacaggcgg ggcagttctt catgcccaat cgcaaatccc
     5041 gccaacagcc tgtgggcttc tgtctgtctt gtgacggcct tccgcgcgag cgatacgacg
     5101 atatagcaat tcctggagtc aagccaagaa tctgggctgg ctgacgaaac tccaatagcc
     5161 tcgattgtgc aacacgacct gtcctgctgc gatcaagcca atgaaaacgg ccgtcatttc
     5221 cccgctgcga ttcgccttcc tcgtcgctct ggttctttgc ccgctcactt tctatgccgt
     5281 ctgggcattc gggccgttcg gctccctgct cattatggcc gtgccagcag cgttcgctct
     5341 gtactttccg cgtcccgact gacacactgg gaggtgtgat ttcccttggc ctcatcattg
     5401 ttgctgcgat ggcgattctc gccatcggcg caattgtcgc cttggtagtg attgcctccg
     5461 ccagccgcgg gcgacacgac gagcgcgacc gttgagcttg gcaatccgcc gccgattggt
     5521 tttcagctcc gcggccgcag attggcgaca tggcctcagc ggcagctcgc cgcctgtggt
     5581 tgccacgatg gtcctggacg aggaattccg cgtgggcg
//
LOCUS       DASRJB010000742         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4164168, whole genome shotgun sequence.
ACCESSION   DASRJB010000742 DASRJB010000000
VERSION     DASRJB010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4164168"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFB80_19530"
     CDS             complement(<1..562)
                     /locus_tag="VFB80_19530"
                     /inference="COORDINATES: protein motif:HMM:NF036842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36035.1"
                     /translation="MKSAFWSPVLALAALVASTGTARGQDRNGDRGFREDDVVLSWNW
                     VMLEADATDHGQGAPDQGGPTRAARAYAIVSAAVFDACNSITHKHRPYLTELQGFERA
                     DKRAAVSSAAYHTLRELYPQQREALDDALDRWLRRIGDSEGRTRGVALGRRVAEAILA
                     ARSDDGSDAPMNYTPTDKPGHHQVDPN"
     gene            complement(810..1823)
                     /locus_tag="VFB80_19535"
     CDS             complement(810..1823)
                     /locus_tag="VFB80_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654571.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HZN36036.1"
                     /translation="MSPCLTQLRAGTASADSGAADWRLAGHRYYSYNLFLRNKFGGRR
                     VQKVSLDAGLTCPNVDGTVAVGGCTFCDNRSFSPSRRVRRQDILTQLDDGMRRLRHRY
                     EGLDQFIAYFQPATNTYAPVGRLRAFYEAALSHPHVVGLAIGTRPDCVPDEVLELLTE
                     IAARSYLSVEYGLQTMHDRSLDWMNRGHHHAATIDAMERSRGRGFEICAHVMLGLPGE
                     SREDMLATARELARLKFDAVKIHNLYAVRNTPLAEQVARGEVTLMGRDEYIATLVDFL
                     ELLPPETVVERISGDAPPDYFIGPSWCLDKPGVLRALHAELERRDTWQGKLRGPEPMS
                     DGP"
     gene            1958..2410
                     /locus_tag="VFB80_19540"
     CDS             1958..2410
                     /locus_tag="VFB80_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN36037.1"
                     /translation="MHGELIPVGGGDPIPLLKETLIIGRRESCDIVLRFANVSGHHCQ
                     LSLESGYWFVQDMNSQNGVKVNGTRVIRKRLDPGDLISMARHKYTIKYTPEGPPPSEE
                     EDLAAILSKSLLDRAGLQRRADGSPRRYMPQEKPPEDAAGEAKKREYE"
     gene            complement(2411..2818)
                     /locus_tag="VFB80_19545"
     CDS             complement(2411..2818)
                     /locus_tag="VFB80_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36038.1"
                     /translation="MLLDTGPLVAFRNPADSQHQICIEMLGQIEPPLITSWSVMTEAA
                     WMLRHDTEVVGRMFRGAAEGLFDIGHLTAEDLPAIDELRRRYADLSPQLADLTLLHLA
                     NRENLDTVFTLDQRDFRVFRAGRKSLRLLPGDE"
     gene            complement(2838..3089)
                     /locus_tag="VFB80_19550"
     CDS             complement(2838..3089)
                     /locus_tag="VFB80_19550"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZN36039.1"
                     /translation="MSQRRISIRITKELEKRLAKAAKKAGKSESEIVRAALDSYCTEE
                     EARPSAYDLFKRAGLIGDAKNLPSDLATNPKYMEGFGRG"
     gene            complement(3149..>3854)
                     /gene="nadC"
                     /locus_tag="VFB80_19555"
     CDS             complement(3149..>3854)
                     /gene="nadC"
                     /locus_tag="VFB80_19555"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZN36040.1"
                     /translation="QPMLSDGDSASAGTLVATLAGSARDLLTSERTILNLLGRLSGIA
                     TLSRQFVQAIAGTKARLYDTRKTIPGWRRLEKYAVLCGGGHNHRTGLFDAILIKDNHL
                     ALGRSEQGAAAYTPAEAVRKARAFLAQQSAGEAPRGAPDAEMLVEIEVDSLDQLRAVL
                     PEKPDLVLLDNMPPDVLREAVRIRDSGGYASELEASGGISLATVRAVAETGVERISSG
                     ALTHSAICLDVALDWQ"
BASE COUNT          663 a         1206 c         1272 g          713 t
ORIGIN      
        1 tgttcggatc cacctggtga tgccccggct tgtcggtcgg cgtgtagttc attggcgcgt
       61 cggagccgtc gtccgaccgc gcggccagga tggcttcggc gacacggcgg ccgagcgcca
      121 cgccgcgggt ccggccctcg ctgtcgccga ttcgcctgag ccagcggtcc aaggcgtcgt
      181 cgagcgcttc gcgctgttgc gggtagagtt cgcggagcgt gtggtaggcg gctgaggaga
      241 cggcggcccg cttgtccgcg cgctcgaagc cctgcagctc ggtcaggtac ggccggtgct
      301 tgtgcgtgat cgagttgcag gcgtcgaaca ccgccgccga gacgatcgcg tacgcgcggg
      361 cggcgcgggt cggaccgcct tgatcgggcg caccctgccc atgatccgtg gcgtcggctt
      421 ccagcattac ccagttccag gacagcacca catcgtcctc gcggaagccg cgatctccgt
      481 tgcgatcctg cccgcgggcc gtgccggttg aggccaccag tgctgccagg gccagcaccg
      541 gactccaaaa tgcgctcttc attttgcgtt ctccttccga ggtgagagaa ggggcgtgat
      601 tatcgcggag ggaaacgatc tggcaatccg gtacgcggct cggctggttc gcgtttcaac
      661 ccggaagcct gcccgattgg cagttgtcgc gcatcttttg cttgacggcc taggggcttt
      721 gggcgagcgc tagcccctct tggacctgct taggcgacgg attgccgtgc tggcaaaccg
      781 cagaagcatg tcgcgaattg cggcgagtct catgggccat cgctcatcgg ttctgggcca
      841 cggagcttgc cctgccaggt gtcgcgccgc tccagctccg cgtgcagcgc ccgtagcaca
      901 cccggcttgt cgaggcacca actggggccg atgaagtaat cgggcggcgc gtcgccgctg
      961 atgcgctcga cgaccgtttc cggcggcagc agctccagga aatcgaccag cgtggcgatg
     1021 tactcgtcgc ggcccatgag cgtcacttca ccgcgcgcga cctgctcggc cagcggcgta
     1081 ttgcggaccg catatagatt gtgaatcttc accgcgtcaa acttcagacg cgccagctcg
     1141 cgggccgtgg ccagcatgtc ctcgcgcgac tcgccgggca ggcccagcat cacatgggcg
     1201 cagatctcaa agccgcgacc gcggctgcgc tccatcgcgt cgattgtcgc cgcatgatgg
     1261 tgtccgcggt tcatccagtc gagcgagcgg tcgtgcatcg tttgcagccc gtactcgacc
     1321 gaaaggtacg accgcgcggc gatctcggtc agcagctcca gcacttcgtc gggcacgcag
     1381 tcgggccgcg tgccgattgc cagacctacc acatgcggat gtgacagggc cgcttcatag
     1441 aaggcccgca gccggccgac cggggcatac gtgttcgtgg ccggctgaaa ataggcaatg
     1501 aactgatcca ggccttcgta gcgatgccgc agccgccgca ttccatcgtc caactgcgtc
     1561 agaatgtctt ggcggcggac ccgccggctg gggctgaagc tgcggttgtc gcaaaacgtg
     1621 cagccgccga cggcgaccgt gccatcgaca ttggggcaag tcaggccggc atcaaggctc
     1681 accttctgaa cgcggcggcc gccgaactta ttgcggagaa agaggttata tgaatagtag
     1741 cggtggccgg ccaaccgcca atcggcagcg cctgagtcgg ccgaagccgt gccggcacga
     1801 agctgcgtca ggcagggaga cataataagt tgacgatgcc tccccggctg cctagattga
     1861 aggtcggcct gatcggcaac agagtatggg cgcggcggca ctccgcagga agcaagccag
     1921 aatagatcgt gccggcggcc ctcacaaggg tcagcatatg catggcgagt tgatccccgt
     1981 gggtgggggc gaccccattc cactgctcaa agaaacgctc atcatcggcc gccgcgaatc
     2041 gtgcgatatc gtactgcggt tcgccaatgt ctccggccac cactgccagc tgtcgctgga
     2101 gagcggctac tggttcgttc aggatatgaa cagtcagaac ggagtgaagg tcaacggcac
     2161 ccgcgtgatt cgcaagcggt tggacccggg cgatctgata tcgatggctc ggcacaaata
     2221 cacgatcaag tacacgcccg agggtccgcc gcccagcgag gaagaggacc tggcggcgat
     2281 tctgagcaag tcgctgctcg atcgcgcggg cttgcagcgc cgggccgacg gcagtccgcg
     2341 gcgctacatg cctcaggaga aaccgcccga agacgccgcg ggcgaggcga agaagcgcga
     2401 gtacgagtag ctattcgtcg ccgggcaaga gccgcagcga tttccgtccc gcccggaaga
     2461 cgcggaagtc tcgctgatcg agcgtgaaga ccgtgtcgag attctcgcgg ttcgcgagat
     2521 ggagcagggt gagatcggcg agttgaggac tcaggtccgc gtaccggcgg cgcagctcgt
     2581 cgatggcggg caagtcttcc gcggtgaggt ggccgatgtc gaacagacct tcggcggcgc
     2641 cgcggaacat tcggccgacg acctccgtgt catgccttaa catccatgcc gcttccgtca
     2701 tcaccgacca ggaggtaatc agcggcggtt cgatctggcc gagcatctct atgcaaattt
     2761 gatgctggct atccgcggga tttcggaatg cgaccaaagg accagtgtcg agcagcactc
     2821 ggcgaaatcg tgctgcctta accacgtccg aagccttcca tgtacttcgg attggttgcc
     2881 aggtccgacg gcagattttt cgcgtcgcca atcaatccgg cacgcttgaa gagatcgtac
     2941 gcggaaggcc tcgcttcttc ctccgtgcaa tagctatcga gcgcggcgcg gacgatctcc
     3001 gattcactct tgccggcctt tttggcggct ttggccaggc gcttctcgag ctctttcgtg
     3061 atgcggatgg agattcggcg ctgggacata acgcaccttt gtcagacatt ctacctcatt
     3121 tgtcagacac ggcaaatggc cttggcgttt actgccaatc cagcgccacg tcgaggcaga
     3181 tggctgagtg cgtcaatgcg ccgctgctga ttcgttcgac gcccgtttcg gctaccgccc
     3241 gcaccgtggc gaggctgatg ccgccggagg cttccaattc actggcgtag ccgccgctgt
     3301 cgcggattct cacggcttcg cgcagcacat cgggcggcat attgtcgagc aggaccaggt
     3361 ccggtttctc gggcaggacg gcccgcaact ggtcgagcga atcgacctcg atttccacga
     3421 gcatctccgc gtcgggcgct ccgcgcggcg cttcgccggc cgactgctgt gccagaaagg
     3481 ccctggcctt gcggacggcc tcggcgggcg tgtaggcagc tgcaccctgt tcgctgcgac
     3541 ctagcgcgag gtggttgtcc ttaatgagga tcgcgtcgaa gagacccgtg cggtggttgt
     3601 ggccgccgcc gcagaggacg gcgtatttct caagccttcg ccagccgggg attgtttttc
     3661 gcgtgtcgta caggcgggct ttcgtgccgg cgatcgcctg gacgaactgc cgactgagcg
     3721 tggcgatgcc cgacaggcgg ccgagcaggt tgagaatcgt ccgttcgcta gtcaacaggt
     3781 cgcgtgccga gccagccagc gtagcgacga gcgtacctgc gctggcgctg tcgccgtcgg
     3841 aaagcatggg ctga
//
LOCUS       DASRJB010000743        18522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_781120, whole genome shotgun sequence.
ACCESSION   DASRJB010000743 DASRJB010000000
VERSION     DASRJB010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18522
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781120"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFB80_19560"
     CDS             complement(<1..312)
                     /locus_tag="VFB80_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36041.1"
                     /translation="MSRLIAFLGLSILVAPLARAEDAWPPELPKAKSGTATLSSPEFL
                     KVPESVAAAAEKAGAKFSVAKSPPTVHLAYHRDLGPEAIKRRLWSSWGDICVASDGRV
                     YC"
     gene            423..1868
                     /locus_tag="VFB80_19565"
     CDS             423..1868
                     /locus_tag="VFB80_19565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN36042.1"
                     /translation="MRNILSISVLLLASPAVAAEARPNIVFILADDLGWTDTGAYGSK
                     YYETPNVDRLASQGMKLTRYHNCQNCQPTRAALMTGQYAPRTGVYTVGGIDRFAWQTR
                     TLRPVDNVQQLPLDKTTIAQSLKAAGYATGMFGKWHLGNPKGYHPADRGFDEAIVSMG
                     QHFNFDTNPKTDYPAGQYLADFLTDKAVDFIRRHKDGPFFLYLPHFGVHSPHEAKPEL
                     IERFRAKPAEGGHKNPTYAAMIASVDESVGRIMALLDELKLADNTVLIFSSDNGGVGG
                     YVREGVKQAGDVTDNAPLRSGKGSLYEGGTRDPFIVRWPGKVAPGSTCDVPTIHVDVF
                     PTFLEIAGASPPAGQTLDGESLVPLFKNASAKLKREAIYQHFPGYLGAGAGSWRTTPV
                     GLIEAGDWKLMEFFEDGRLELYNLKDDIGETTNLASKMPEKTKELHARLLAWRAEIKA
                     PMPTPNKPTDAPPPAAKKGKKAKAKAKVGSN"
     gene            1882..2409
                     /locus_tag="VFB80_19570"
     CDS             1882..2409
                     /locus_tag="VFB80_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36043.1"
                     /translation="MSARMGLAAVIVLAGAAAVRAQLQVQADKENVVASKLEGQWEPD
                     VELTRRLVGSVPVPPAGQADKNPARLIVKSEPAVAAKIPAKFARFFEGKQIYMAGLIT
                     LQDKERPFVLIEHKGNPHLVWFREQGGEALGDAESFNVMLAVAKQTQDDLLFIGGDFN
                     NQPFSALRRAKAEPK"
     gene            2450..2845
                     /locus_tag="VFB80_19575"
     CDS             2450..2845
                     /locus_tag="VFB80_19575"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HZN36044.1"
                     /translation="MTMLRRLRPAFSAALLVAAAAVRILAADPTQTVRLTIDYGDGVQ
                     KTFLNLEWKEKLTVFDALKAAEKHPRGIKVEHSGSGETIFIKTIDDAPNEGQGGRNWR
                     YSVNGEGGKVSAGIAELKPGDQIVWRYAR"
     gene            complement(2852..4849)
                     /locus_tag="VFB80_19580"
     CDS             complement(2852..4849)
                     /locus_tag="VFB80_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36045.1"
                     /translation="MSHSSRRVRSKIERAAPPRMAIKAAVALAVCLGLRVVLAQGPIA
                     NEEVDANGFKYLVPHSFPGKTQIEAKKAANDLKNRISSMLFSPEPLGEGRGYFERYYT
                     LVYFPQLTQTSVEALKALPDERQRFFRNHLEYCKSNDAHQVLVAVTLQEMNRIVSENF
                     HPAVRYNAMLIISGLNDKEVQRVQPYAVPEPMVRALKVILDLYRACKGPEDDGIRLAA
                     LLGMSRHLEWEHYKNEGSPPAPIQPALRKEIMDELFKLAQTKDPPPGRDAEGHLWFRR
                     RAVEALGHSCSKIADPAVASALEGLLRDEKEPLSLRLTVAATIGRVSLQNPVAVDAKA
                     LSTEMGYLALVALDTELSRVSNLRKTEEEHFIRLSGQVPLEGEQPGVINPRAYAPGSS
                     PDGGPGLRGLGGVPGPGSGAYGRGPGMPGAMPGDYGDPSAVDPLSLDPKGYRFAPVRK
                     RLRYDLYCVQIGLLGGEDHAPPKNASAPPPPMAPKAPMTPTTPAADKGPPPVPRGLFA
                     VAKAGPDKTYVDDVYAKVRKLADLVENRSSDLFQMDRDLRREMKNLEAITRKLAPPAA
                     PAAAAAAAAAEEADLLTPPVRGGAAPPMPMPPAAPAPGAAAAPAPMPKGAAPMPPAAA
                     PMPPAAAPMPPAGPMPKGNTPAAPAPMPGAPAPPPMPAPAP"
     gene            complement(5085..9164)
                     /gene="hrpA"
                     /locus_tag="VFB80_19585"
     CDS             complement(5085..9164)
                     /gene="hrpA"
                     /locus_tag="VFB80_19585"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZN36046.1"
                     /translation="MSAFLRDLEARIDSALQADRHRLRNLLRAVRQAEENGRPPDERL
                     ERLLKQLDESSAKCEARAQFVPRLIYDESLPVVAHREEIAAAIREHQVVVVCGETGSG
                     KSTQLPKICLEIGRGIAGMIGHTQPRRIAARSIASRVAEELSTSVGQKVGFKIRFTDT
                     TGDKTLVKVMTDGVLLAESQHDRFLDQYDTLIIDEAHERSLNIDFLLGYVHRLLPRRP
                     DLRLIITSATIDAQRFAEHFGRPGHPHRGSAPREAGVSRSETTTMAPIIEVSGRTYPV
                     EVRYRPLVREETGEEVEALRGLADAVEEVCRIGAGDVLVFLPTERDILEAAQKLRGRN
                     LPGGQPEILPLYARLSTAEQNRVFAPHSGRRIVLATNVAESSLTVPGIRYVVDTGTAR
                     ISRYSARSKVQRLPIEDVSQASADQRKGRCGRVGPGVCIRLYSEEDFLARERYTSPEI
                     QRTNLASVILQLLALDLGPIDEFPFLDPPRGEAIRDGYKTLFELGAIDEQNRLLPIGR
                     QLARLPADPRIGRMILEASREHCLADVLIIAAALEVQDPRNRPAERAGEADAAHSQWN
                     DPDSDFLSWLRLWDFVQHLRSQTTRGQFRKACQQHFLSELRLREWQDVHRQLLEMVQS
                     FGLKVGKRKWTANQASGRRQPPDSASPSSGDDHREANASRSPAYAAIHRSLLAGLLSS
                     VANRGETSEYTAAGGGKFFLWPGSGAAANKPKWIVAGEMVETSRRYLRTIARIDPAWI
                     EPLATHLVTRSYSDPHWDRQAGGAMAAEKVSLFGLTIVPRRRVRYTAIDPKVSRQLFI
                     RHGLVDGDLDTRAEFFHHNRRLALEIEAQAAKMRRRELIVDPQIVCDWYEARLPAAVV
                     DLPSLDRWRKDAERANRRVLFLGEEDLLGGQSASAPSEEYPDELRIDRLKLPLAYHFE
                     PGAEQDGVTVTVPREGLSQLSEERLGWLVPGLVADKVEALIRSLPKAVRRNFGPAPEI
                     SRKIAAEIGFASGPLLPLVASALSQAAGERIAPEMFDLERLPPHLRMKVRVVDRGGQA
                     IIEGRSLAAIREHLGDQSAPEQPAGSSPWHRDGVTKWDFGKLPEHVDLRRGGLMLTKY
                     PALIDAGDTAALRLLDSRAEAAAQTRLGALRLFALAENRELKTQVAWLPQLEKLRLHA
                     APLANERRIEDQLLDLIAARAYYPESGNAAEGRNATEGVPYSAEEFEARRLLGRKNIL
                     PAVQEVTRLILPLLENYHELRLALEQARLPAWQYAVDDIRGQLAALTAPGFLVSTPWE
                     WLLHYPRYLKAAASRLKKLASGGGPRDRQGHEEVAPRKQACEQRAADHRRRNHANPLL
                     AHYRWLLEELRVSLFAQELGTSQPVSPQRLDKLFEKIPM"
     gene            complement(9237..10340)
                     /locus_tag="VFB80_19590"
     CDS             complement(9237..10340)
                     /locus_tag="VFB80_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HZN36047.1"
                     /translation="MSQLPKPRPVVALHPPQSLTPARFQREREIAVPLPLLVTGVAGV
                     AGYNAFRYFRQRYGKLVIGIRRKDNWPLRGEGLLACDGDDRDTLAQLFDKHQFAAVLN
                     AEGTCKLKSCELDPRMAERVNIDSVTAMLAVIRGTATRLVHLSIDLVFSGTRGGGHVE
                     DDATDPVTVYGKTMAEAERILAKTAAAVLRISLPMGVSFNGHAGAIDWIQSRFKKGKP
                     ATLYFDELRTPTYTDCLSPLLATVLARKDITGLFHAGGPRRLSLFQIAQIVNRIGGYD
                     PHLLHGCPRADAGPLPPRAGDVTMDSSKLSAALGYDPFDPWPLHDDHVPTHRDWHKEG
                     PRGSPELLAEVLYRNPRGCAVGNALRGVPCAAD"
     gene            complement(10340..13309)
                     /locus_tag="VFB80_19595"
     CDS             complement(10340..13309)
                     /locus_tag="VFB80_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN36048.1"
                     /translation="MPAELLPEDLPSRYVVGIDLGTTNSAVCYVDTAEQPWRVRVLEI
                     PQLVAPGQVEARDTLPSFHYQPPQGEEPKNQRTEEPSTKHETPNAAYVVGFMARDYGA
                     ATPGRLISSAKSWLCHTGVDRTAELLPWHAAADVERISPVEASSRYLAHIRGAWNERF
                     KAEPLERQDIVLTLPASFDEIARELTVQAAARAELARVVLIEEPQAAFYAWIYKHQDN
                     WEKLVAPGQKILVCDIGGGTTDFTLIRVRRTGASDGTNATDETDGARVQFHRVAVGDH
                     LLLGGDNMDLALAQYLEQELLRPASGRREPPDNPIGGNLVENYREADASRSPARAKLQ
                     PRQWDTLLRQARQVKELLLSDSGPGELTVTIPGSGSKLIGGALQARVTRDEVHKLLVD
                     GFMPRASLSDKPAARRSGFQEFGLPYAADPAITRYLAAFLTAHRLAGEEGSGFGVQGS
                     AAEVDPARPDVLLFNGGVFESGVLQERLIDSLSSWFGGDDWKPAVLANDRLDLAVARG
                     AAYYGMVRRGEGVRIAASLARTYYIGVEADPPAALCLVPGSAEPGQSIELDRQFDLLV
                     SEPVEFPLYTSSIRLTDRPGELVPVDPEQMTALPPIRTALKTRTRRERGAVAVKLNAK
                     LTEIGTLELWCSEAAGERTWRLQFDVRSATQTDVAAHETDAESQGFIDEETAAACRVV
                     LESTFGATGRDDPNPLVKRLVAAAGSDRGEWPTSLLRRLWEMLVEWEAGRRNSPAHEA
                     RWLNLLGYCLRPGYGLAVDDWRVAETWKTVQGKLAHAAASSRTESLILWRRIAGGLPA
                     GQQRALAEPLLATVRTLHKRQTSGQGKGTDPTFSPHETLEVLRLLGSLELLAGEVKID
                     LGGLLLDLLPKKKLEAIRPACLWSLGRLGAREPAYGPLNTVVPPETASEWIERLLEME
                     LVDAMTQFALVNLARKTGDRFRDINDALRSLVLDWLGERSAGEHARQLVSEGGHLDVQ
                     EQGRVFGESLPKGLRVR"
     gene            complement(13371..15161)
                     /locus_tag="VFB80_19600"
     CDS             complement(13371..15161)
                     /locus_tag="VFB80_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN36049.1"
                     /translation="MPAKYVIGIDLGTTNSVVACASLSEEQPRVELLPIPQLFAPGVV
                     ESRTALPSFLYLAPEHEAKGGAFELPWCKGNNFVVGELARRQSAESPQRTVAGAKSWL
                     AHSKVDRHQSILPWQAPAEVPKVSPVTASRRYLEHLIAAWEHAHPEAPVAEQLVVLTV
                     PASFDAAARELTREAALTAGLPASLILLEEPQAAVYAWLTAAGEKWRRLLRVGETLLV
                     CDVGGGTTDLTLVGVDQEGGELTLRRLAVGEHLLVGGDNMDLALAYFVAGKFGEKGVK
                     LDPWQSISLWHQCRTAKEALLTGGGPKKHPLSVLGRGSKLIGGTVKVEIERDAAAELL
                     LEGFFPKCQITDRPGRRRASGFQEIGLPFESDAAVTRHLAAFLAAHGKAGEPIRPAHV
                     LFNGGVFKADAFRSRLLDVLSDWTGDAPPRTLAGEQDLDHAVARGAAYYGWAKQHGGV
                     RIRGGTARSYYVGIETSGLAIPGAPRPLRALCVVPFGLEEGTSIDVPSSEIGLVVGES
                     ATFRFFSSAVRKQDQPGDLLPAWTDEELAETDSLEAVLPRDEAIDDDFVPVRFQSQLT
                     ELGVFELWCQSTKSPNRWKLEFSVRKDADA"
     gene            complement(15211..17739)
                     /locus_tag="VFB80_19605"
     CDS             complement(15211..17739)
                     /locus_tag="VFB80_19605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HZN36050.1"
                     /translation="MKIAIPELCLVVLIGPSGAGKSTFARKHFKATEVLSSDFCRGLV
                     SDDENDQAATAAAFEILHAIASKRLAAGKLTVIDATNVQTEARQPLLSLAKSHDVLPV
                     AIVFNLREELCASRNSGRADRTFGPHVIRNQVRQLRRSLKQLAREGFRHVFTFNDPAQ
                     IDAATIEREKLWNDMRHERGPFDIIGDVHGCADELEELLTRLGYSSSASGSSWAHPAG
                     RQAIFVGDLVDRGPRVVAVLDLVRAMVTSGAAWCVPGNHDMKLLKHLQGRQVHVTHGL
                     EQTLAEIEALPPDSREAWKAQTAAFLDSLVSHYVFDGGRLVVAHAGMKAEYQGRTSRR
                     VREFALYGETTGETDEFGLPVRLNWAAEYRGQAMVVYGHTPVPEPEWLNHTINIDSGC
                     VFGGKLTALRYPERELVSIPARRIYCEPARPFLPAPSALTAQQQLDDLLDIEDCLGKR
                     LIETRLAGRVTIRAEQSAAALEVMSRFAVNPKWLIYLPPTMSPCETVPEGELLEHPDQ
                     VFDYFRGAGIQHVVCQEKHMGSRAVVVLARDEAAAQKRFGVTDEGRGIIVTRTGRRFF
                     DEPQLESDMLDALWSAMTAAGWWERFQSDWFCLDCELMPWSAKAQELLQHQYAPVAAA
                     GEASLREAAALLASHQPIAGQLQERLACASLYRAAYGRYCWPVRSAADLRLAPFHLLA
                     AEGQVFTHHDHLWHMQTLSELAAVPLIQATRHLLVDLHDAASTTAATTWWEELTTAGG
                     EGMVVKPREWIAAGPKGLVQPAMKCRGREYLRIIYGPEYTLSEHLARLRKRSTLAKRS
                     LASREFALGIEALERFVGREPLRRVHECVFGVLALESEPVDPRL"
     gene            complement(17749..>18522)
                     /locus_tag="VFB80_19610"
     CDS             complement(17749..>18522)
                     /locus_tag="VFB80_19610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HZN36051.1"
                     /translation="EEWLARHPRREEIAQRYLRYRAPLTRAALARLPADEPVEDEEEA
                     ADKTAAEELVEQPLRLNEQRLLAVVAALVQSGAQRVLDLGCSTGNLLRKLLEEPQFSE
                     IVGLDVSHRALAAAERRLKLDRLPAAKAQRIKLLHGSLTYRDRRLAGYDAAALVEVIE
                     HLDPPRLRALERAVFECARPKTVVVTTPNREYNVLFPGMPPGKLRHKDHRFEWTRDEF
                     RSWSERVAAQFGYGVQLSGIGPADSHHGSPTQMATFTST"
BASE COUNT         3061 a         6197 c         6153 g         3111 t
ORIGIN      
        1 acaatagacc cggccatcgc tggccacgca gatgtcgccc cagctcgacc agagccgccg
       61 cttgatcgcc tccgggccga ggtcccggtg ataggccagg tgaactgtcg gcggcgactt
      121 ggccacgctg aatttcgcgc ccgccttctc cgcagcggct gccacgcttt caggaacctt
      181 cagaaactcc ggggaactca gcgtggccgt gccgctcttg gccttcggca actccggcgg
      241 ccaggcgtct tcggcgcgag ccagtggagc cacaagaatc gacaagccaa ggaaagcaat
      301 caggcgggac atgacagcgg cgggtaggcg gggcaagggc gcgaggttga cattataata
      361 gcccgactcg atcgctgccc aacgtcgcct cttggcgcct ggcgttaaat cttggagtca
      421 tcatgagaaa catcctctcc atttcggtct tgttgctggc cagtcccgct gtcgccgcag
      481 aggctcgccc gaacattgtc ttcatcctgg ccgacgatct cggctggacc gacacgggcg
      541 cgtacggcag caagtactac gagacgccga acgtcgatcg attggcgtcg caggggatga
      601 agctcacgcg gtaccacaac tgccagaact gccagccgac gcgggcggcc ctgatgaccg
      661 ggcaatacgc tccgcgaacc ggcgtctaca ccgtcggcgg catcgatcgc tttgcctggc
      721 agactcgcac gctgcggccg gtcgataacg tgcagcagct gccgctcgac aagacgacga
      781 tcgcccagtc gctgaaagcc gccggctacg cgaccggtat gttcggcaag tggcacctgg
      841 gcaaccccaa gggctaccat ccggccgatc gcggcttcga cgaggcgatc gtcagcatgg
      901 ggcagcactt caacttcgac acgaacccca aaaccgacta tcccgcggga cagtacctgg
      961 ccgactttct gaccgacaag gcggtcgatt tcatccgccg gcacaaggac gggccgttct
     1021 tcctttacct cccgcacttt ggcgtccact cgccgcacga ggcgaaaccg gagctgatcg
     1081 agcggttcag ggccaagccg gcggaaggcg ggcacaagaa cccgacctac gcggcgatga
     1141 tcgccagcgt cgatgagagc gtcggccgga tcatggccct gctcgacgag ctgaagctgg
     1201 cggacaacac ggtgctgatc ttttccagcg acaacggcgg cgtcggcggc tatgtgcgcg
     1261 agggcgtgaa gcaggcgggc gacgtgaccg acaacgcgcc gctccgcagc ggcaaaggga
     1321 gtttgtatga aggcggtacg cgcgatccat tcatcgttcg ctggccggga aaagttgccc
     1381 caggctccac ctgcgacgtc ccgacgatcc acgttgacgt cttcccgacg ttcctggaga
     1441 tcgccggcgc ttcgccgccg gccggccaga cgctggacgg cgagagcctt gtgccgctct
     1501 tcaagaacgc gagtgcgaag ctgaagcgcg aggccatcta tcagcatttt ccgggctacc
     1561 tcggggctgg agcgggcagc tggcggacga cgcccgtagg cctgatcgaa gccggcgact
     1621 ggaagctgat ggagttcttc gaggacggcc gcctcgagct gtataacctg aaagacgaca
     1681 ttggcgagac gacaaacctc gccagtaaga tgcccgagaa gaccaaggaa ctgcacgcca
     1741 ggctgctcgc ctggcgggcc gagatcaaag ccccgatgcc cacccccaac aagccgaccg
     1801 acgcgccgcc ccccgcggcc aagaagggaa agaaggccaa ggcgaaggcc aaagtcggca
     1861 gcaattagcg cggagattgc catgagcgcg agaatgggat tggcggccgt catcgttctg
     1921 gctggtgctg cggcggtccg ggcgcagctg caagtgcaag cggacaagga gaacgtggtc
     1981 gccagcaagc tcgaggggca atgggagccg gacgtggaac tgaccaggcg cctggtgggc
     2041 agcgtccccg tcccgcccgc cggccaggcc gacaagaatc ctgccaggct gatcgtcaag
     2101 agcgagcccg ccgtggcggc caagattccg gccaagtttg ccaggttctt cgagggcaag
     2161 caaatctaca tggccggcct gatcaccctt caggacaagg agcgtccttt cgtgctcatc
     2221 gaacacaagg gcaacccgca cctcgtctgg ttccgcgagc agggcggcga agcgctgggc
     2281 gatgcggaat cgttcaacgt catgctcgcc gtcgcgaaac agacgcaaga cgatctgctg
     2341 ttcatcggcg gcgacttcaa caaccagccc ttctcggcgc tgcgccgcgc gaaagccgaa
     2401 cccaagtgat ccccagaaac gttcacccgc tgctgccagg gacaatccca tgacgatgct
     2461 gcgtcgcctt cgaccagctt tttcggcggc gctgctggtt gccgccgccg ccgtgcgaat
     2521 tctggccgcc gaccctaccc aaaccgtccg gctgacgatc gactatggcg atggcgtgca
     2581 gaagaccttc ctgaacctgg agtggaagga gaagctgacg gtgtttgacg ccttgaaggc
     2641 cgcggagaag catccgcggg ggatcaaggt cgagcacagc ggcagcggcg agacgatctt
     2701 catcaagacc atcgacgacg cccccaatga aggccagggc ggccgcaact ggcggtacag
     2761 cgtcaacggc gaagggggca aagtcagcgc cgggatcgcc gagctcaagc ccggcgatca
     2821 gattgtctgg cgttacgcgc gctgacgacg gctacggcgc tggcgcaggc atcggcggcg
     2881 gagcgggggc cccaggcatc ggcgcggggg cagccggcgt gttgcccttc ggcatcggac
     2941 ccgcgggcgg catgggcgcg gccgccggcg gcatcggggc agccgcaggc ggcattggcg
     3001 cagcgccttt gggcatcggt gccggagcgg ccgcagcgcc gggcgccggg gccgcgggcg
     3061 gcatcggcat gggcggcgca gcgccgcctc ggaccggcgg agtcagcaaa tccgcctctt
     3121 cggcggcagc ggccgcagca gccgcggcgg gcgcggccgg cggagcgagc ttccgcgtga
     3181 tcgcttccag gttcttcatc tcccggcgga gatcgcgatc catctggaac agatcgctcg
     3241 agcggttctc gaccaggtcg gcgagcttgc ggactttggc atacacgtcg tccacatagg
     3301 tcttatcggg tccggccttg gccacggcga acagaccgcg cgggaccggc ggcggcccct
     3361 tgtcggcggc cggcgtcgtc ggcgtcatgg gcgctttcgg cgccatcggc ggaggcggag
     3421 ccgaagcgtt tttgggcgga gcatgatcct cgccgccgag caggccgatc tgcacgcagt
     3481 acaggtcgta ccgcaaacgc ttgcggacgg gcgcgaagcg gtagcccttg ggatccagcg
     3541 acaagggatc aacggcgctg gggtcgccat aatctccggg catcgcgccg ggcatgcctg
     3601 gtcccctgcc atatgccccg ctgcccgggc ccggaactcc gccgaggccc cgcaggccgg
     3661 gaccaccgtc gggactgctc cccggagcat aggcacgcgg attgatgact cccggctgct
     3721 cgccctccag cggcacttgc ccggacagcc ggatgaagtg ctcttcctcc gtcttccgca
     3781 ggttgctcac gcggctgagc tccgtgtcga gggcgacgag cgccaaatag cccatttcgg
     3841 tgctgagggc cttggcatcg acggcgaccg ggttctgcag cgagacgcgg ccgatcgtcg
     3901 cggcgaccgt caagcgcagc gaaagtggct ccttttcgtc gcggagcagc ccttcgagag
     3961 cggacgccac ggcgggatcg gcgattttcg agcaggaatg gcccagcgcc tcgacggcgc
     4021 ggcggcggaa ccagaggtgc ccctccgcat cgcggcctgg cggcgggtcc ttcgtctggg
     4081 ccagcttgaa cagctcgtcc atgatttcct tgcgcagtgc aggctgaatc ggcgccggag
     4141 gagagccctc gttcttgtaa tgctcccact ccaggtgccg ggacattccc aagagtgcgg
     4201 ccaggcggat tccgtcgtcc tccgggccct tgcaggcgcg atacaggtcg aggatcacct
     4261 tcagggcccg gaccatcggc tcgggcacag cgtagggctg tacccgctgc acttccttgt
     4321 cgttgagtcc gctgatgatg agcatcgcgt tgtagcggac cgcgggatga aagttctcgc
     4381 tcacgatgcg gttcatctct tgcagcgtca cagcaacgag aacctggtgc gcgtcgtttg
     4441 acttgcagta ctccagatgg ttgcggaaga atcgctgccg ctcgtcgggc agcgccttga
     4501 gcgcttccac cgaggtttgc gtgagttggg ggaaatagac cagcgtgtaa tagcgctcga
     4561 agtacccccg gccctcgccc agcggctctg ggctgaacag catggagcta atccggtttt
     4621 tcaggtcatt ggccgccttt ttggcttcaa tctgagtctt gccggggaac gagtgcggca
     4681 cgaggtactt gaagccgttg gcatcgacct cctcgttggc gattggaccc tgcgccaaga
     4741 caacccttag gcccaggcag actgccaggg cgaccgctgc cttgatcgcc attcgcggcg
     4801 gggcagcgcg ctcgatcttc gaacgaactc tgcgactgga atgcgacatg taaccaacct
     4861 ccgttcgccg caggattgcg gcgagatcca gaaacgatcg ctcggctcaa cgcggacgat
     4921 tgtccgccgg gctggagcgg ttgagccgac ccctgttcct tgcgtggaag acccgccacc
     4981 gcggaacctt cctcaattga taacgtacct acggcgcttg acgaaagtca actcaagcgg
     5041 acccgggaac cccaaaaccg gcccggaact gcggattgtc cgggctacat ggggattttc
     5101 tcgaacaact tgtccagccg ctgcggcgag acgggttggc tggtgcccag ttcctgagcg
     5161 aacaacgaca cgcgcagctc ttccagcagc cagcggtagt gggccagcag cgggttggcg
     5221 tggttgcgtc ggcggtgatc ggcggcccgc tgctcgcagg cctgcttccg cggagcaacc
     5281 tcttcgtggc cttgacggtc ccgcgggccg ccgccgctgg ccagcttctt cagccgcgag
     5341 gcggcagcct tgagatagcg cggataatgc agcagccact cccagggagt ggagacgaga
     5401 aagccgggtg ctgttagcgc cgcgagctgg ccgcggatgt cgtccaccgc atattgccag
     5461 gccggcaggc gcgcctgctc cagcgctagc cgcagctcgt gatagttttc caggagcggc
     5521 agaatcagcc tggtaacttc ctggaccgcc ggcagaatgt tcttgcgtcc caaaagtcgc
     5581 cgtgcttcaa actcctccgc gctgtaggga acgccctctg tggcgttccg gccctccgcg
     5641 gcgttcccgg attccgggta atacgccctc gccgcaatca ggtccaagag ctgatcctcg
     5701 atccgccgct cgttggccag cggcgcggcg tgcaggcgca gcttctcgag ctgcggcagc
     5761 caggcgacct gcgtcttcag ctcgcgattt tccgccaggg caaacagccg cagtgcgccc
     5821 agccgcgtct gggcagcggc ttcagcccgc gagtcgagca gtcgcagtgc cgcggtgtca
     5881 ccggcgtcga tgagcgccgg gtacttggtg agcatcaatc cgccgcgccg caaatcgacg
     5941 tgctcgggca acttgccgaa atcccacttc gtcacgccgt cgcggtgcca gggcgaactg
     6001 ccggcaggct gctcaggcgc ggactgatct cccaggtgct cgcggatggc cgccaggctg
     6061 cggccttcga tgatcgcttg gccaccgcga tctacgactc gcactttcat ccgcagatgc
     6121 ggcggcagcc gctccaggtc gaacatctcg ggcgcgatcc gctcgccggc ggcctggctc
     6181 aaggcactgg cgaccagcgg taacaatggc ccggaggcga agccgatttc agcagcgatt
     6241 tttcgcgaaa tctccggagc ggggccgaaa ttgcgccgca cggccttggg aagcgagcga
     6301 atcagcgcct cgaccttgtc ggcgacgagg ccgggcacga gccagcccag ccgctcttcc
     6361 gagagctgcg aaaggccttc ccgcgggacc gtgaccgtca cgccgtcctg ctcggcgccc
     6421 ggctcgaagt gataggccag cggcagcttc aggcggtcga tccgcaattc atcgggatac
     6481 tcctccgatg gtgctgaagc ggactggccg ccgagcaggt cttcttcccc cagaaacaac
     6541 acgcggcgat tggcccgttc ggcgtccttc cgccagcggt cgaggctcgg cagatcaacg
     6601 accgcggccg gcagccgggc ttcataccag tcgcagacga tctgcggatc gacgatcagc
     6661 tcgcgccgcc gcatcttggc cgcctgggct tcgatctcga gggccagccg gcggttgtgg
     6721 tgaaaaaact cggcccgcgt gtcgagatcg ccgtcgacca ggccgtggcg gatgaaaagc
     6781 tgccgcgaga ctttggggtc gatggccgtg taacgcaccc gtcgccgcgg cacgatcgtc
     6841 agaccaaaca gcgagacctt ctcggccgcc atggctccgc cggcctggcg gtcccagtgc
     6901 ggatcggagt agctgcgagt cacgaggtgc gtcgctagcg gctcaatcca cgccggatcg
     6961 atgcgggcga tcgtccgcag atagcgccgc gacgtttcga ccatttcgcc ggccacgatc
     7021 cacttgggct tattcgccgc cgccccgctg cccggccaca ggaagaactt tccgcctcct
     7081 gctgccgtgt attcactcgt ctcgccgcgg ttggcgacgc tggagagcag gccggccaag
     7141 agcgagcggt ggatcgcggc gtaggccggc gagcgggagg cgttagcctc ccgatgatcg
     7201 tctccactcg aaggcgaagc gctatcggga ggctgacgcc tcccgctcgc ctgattggcg
     7261 gtccactttc gcttacccac cttgagcccg aacgattgca ccatttccag cagctgccgg
     7321 tgcacgtcct gccattcccg cagccgcagc tccgacagga agtgctgctg gcaggctttg
     7381 cggaattgcc cgcgcgtggt ctggctccgc aagtgctgca cgaaatccca cagccgcagc
     7441 caactgagga agtccgagtc ggggtcgttc cactgggagt gggccgcgtc ggcctcgccc
     7501 gcacgttcgg ccggtcggtt gcgcgggtct tgcacttcca gggctgccgc gatgatcagc
     7561 acgtcggcca gacagtgctc gcggctggct tccaggatca tgcggccgat ccgcgggtcg
     7621 gcgggcagtc tggccagttg ccggccgatg ggcagcaagc ggttctgttc atcgattgcc
     7681 cccagctcga acagcgtctt gtagccgtcg cggatcgcct cgccccgcgg gggatcgagg
     7741 aacgggaatt cgtcgatcgg ccccaggtcg agcgcgagca gttgcaggat gaccgatgcc
     7801 aggttcgtcc gctgaatctc gggcgaagtg tagcgctcgc gcgctagaaa gtcttcctcg
     7861 ctgtaaagcc ggatgcagac gccgggcccg acgcggccgc agcggccctt ccgctgatcg
     7921 gccgaggctt gcgaaacgtc ctcgatcggc agccgctgga ctttgctcct ggccgaatag
     7981 cggctgatgc gggcggtgcc cgtatcgacg acgtagcgga tcccgggcac ggtgagcgag
     8041 gactcggcaa cgttggtcgc cagcacgatt cgccggccgc tgtgcggcgc gaaaacgcgg
     8101 ttctgctcgg cggtcgatag ccgggcatag agcggcagaa tctccggctg cccgccgggc
     8161 aggttccggc cgcggagttt ctgggccgcc tcgagaatgt cccgctcggt cggcaggaag
     8221 acgagcacat cgccggcgcc gattcggcag acttcctcga ccgcgtccgc gaggccccgc
     8281 agcgcctcga cttcctcgcc ggtctcttcc cgcaccagcg gccggtagcg gacctcgact
     8341 ggatacgtgc ggccggagac ttcgatgatg ggggccatag tagtcgtctc gctccgcgag
     8401 acgccggcct cacgcggagc cgacccgcga tgcgggtgcc ccggtctacc aaaatgctct
     8461 gcgaaccgct gggcgtcgat cgtggcgctg gtgatgatca gtcgcaggtc cggccgccgc
     8521 ggcaacaggc ggtgcacgta gcccagcaga aagtcgatgt tcagcgagcg ctcgtgggcc
     8581 tcgtcgatga tcagcgtgtc gtactggtcg aggaagcggt cgtgctgcga ttcggccaaa
     8641 aggacgccgt cggtcatcac cttgaccagc gtcttgtcgc cggtcgtgtc ggtgaagcgg
     8701 attttgaagc cgaccttctg gccgaccgac gtgctcaact cctccgccac gcggctggcg
     8761 atcgagcggg ccgcgatccg ccgcggctgc gtgtggccga tcatgccggc aatgccgcgg
     8821 ccgatttcca ggcagatttt gggaagttgc gtcgatttgc cgctgcccgt ctcgccgcag
     8881 acgacgacga cctggtgctc gcggatcgcc gccgcgatct cttcgcggtg tgcgacgacc
     8941 ggcagcgact cgtcgtagat cagccgcggg acgaactgcg ctcgagcctc gcacttggcg
     9001 ctggactcgt cgagttgctt cagcagtctt tccagccgct catccggcgg ccggccgttc
     9061 tcctccgcct gccgcaccgc gcggagcaga ttccgcaggc ggtgacggtc ggcctgcagg
     9121 gccgagtcga tgcgggcttc gagatcgcga aggaaggcag acatgcgtcg aaaacatcgc
     9181 cctcgtctgg cgctgtaggg atcgccctct gtggcgatcc gaatcgcgga ttggcctcaa
     9241 tcggcggcgc agggaacgcc acggagggcg ttccctacag cgcagccgcg cgggttgcgg
     9301 tagagtactt ccgcgagcag ctcaggcgat ccccgcggac cttccttatg ccagtcacgg
     9361 tgcgtgggca cgtggtcgtc gtggagcggc caggggtcga acgggtcgta gccgagcgcc
     9421 gcggacagct tgctgctgtc catcgtcacg tcgccggctc gcggcgggag gggtccggca
     9481 tcggcccgcg ggcagccgtg cagcaggtgc gggtcgtagc cgccgatgcg gttgacgatt
     9541 tgggcgatct gaaacaggct gagccgcctt ggcccaccgg cgtgaaacag gccggtgatg
     9601 tcctttcgag ccagcaccgt cgccaggagc gggctcaagc aatcggtata agtcggagtc
     9661 cggagctcat cgaagtaaag cgtcgccggc ttgcccttct taaagcggct ctgaatccag
     9721 tcgatcgccc ccgcgtggcc gttgaaactc acgcccatcg gcagcgagat gcggaggacg
     9781 gccgccgccg ttttcgccaa gatgcgttcc gcctcggcca tcgtcttgcc gtacacggtg
     9841 accgggtcgg tcgcatcgtc ctcgacgtgg ccgccgccgc gggtgccgga gaaaacaaga
     9901 tcgatcgaca ggtgcaccag ccgcgtcgcc gtgccacgga tcacagcgag catcgccgtg
     9961 acgctgtcga tgttcacccg ctcggccatc cgcgggtcga gctcgcacga cttgagcttg
    10021 caggtgcctt cggcgttgag caccgcagcg aactggtgct tgtcgaagag ctgcgcgagc
    10081 gtgtcccggt cgtcgccgtc gcaggccagg aggccttcgc cgcgcagcgg ccagttgtcc
    10141 ttgcggcgaa tgccgattac cagctttccg taccgctggc gaaaatagcg aaacgcgttg
    10201 tagcccgcca cgccggccac gccggtgacc agcagcggga gggggacggc gatttccctc
    10261 tcccgctgga agcgggccgg ggtgaggctc tgcggcgggt gcagcgcgac cactggtcgc
    10321 ggctttggaa gttggctcat caccttaccc gcagtccttt cggcaacgac tcaccaaaga
    10381 cgcggccctg ctcctgcacg tcgaggtgcc cgccttccga caccagctgc cgggcgtgct
    10441 cgccagcgct gcgctcgccg agccagtcta gtacgaggct gcgcagggca tcgttgatgt
    10501 cgcgaaatcg gtcgcccgtc ttgcgggcca gatttacaag ggcaaattgc gtcatcgcgt
    10561 caaccaattc catctccagc agcctctcga tccactcgct ggccgtctcg ggcggcacaa
    10621 ccgtgttcag cggcccatac gccggctcgc gagcacccag gcggcccagc gaccacaggc
    10681 aagccgggcg gatcgcctcc agcttcttct tcggcagcaa atcgagcagc aatccgccca
    10741 gatcgatttt gacctcgccc gccaaaagct ccaaggaccc cagcagccgc aggacttcga
    10801 gcgtttcgtg cgggctgaag gtcgggtcgg tcccctttcc ctggccgctg gtctgacgct
    10861 tgtgcagcgt ccgcacggtg gccagcagcg gctcggccag cgcccgctgc tgccccgcgg
    10921 gaagcccgcc ggcgatccgc cgccacagga tcagcgactc ggtgcggctg ctggcggcgg
    10981 cgtgcgccag cttcccctgc acggtcttcc aggtctcggc gacccgccag tcatcgacgg
    11041 ccaggccata gcccgggcgc aggcaatagc ccagcagatt cagccagcgg gcctcgtggg
    11101 ccggcgaatt gcggcgcccc gcctcccatt ccaccagcat ttcccacagc cggcggagca
    11161 gcgacgtggg ccactcgccg cggtcgctac cggcggcagc gaccaggcgc ttgacgagcg
    11221 gattggggtc gtcgcggccg gttgccccga aggttgattc cagcaccacg cggcaagcgg
    11281 cggcggtctc ttcgtcgatg aagccctggg actcggcgtc ggtctcgtgg gcagccacat
    11341 cggtttgcgt ggccgagcgg acatcgaact ggagccgcca ggtccgctcg cctgcggcct
    11401 cgctgcacca cagctccagc gtgccgatct cggtcagctt cgcattcaac ttcacggcga
    11461 ctgcgccgcg ctcgcggcgg gtgcgagttt tgagggccgt gcggatgggc ggcagagcgg
    11521 tcatctgctc cggatcgacg ggcacgagtt cgcccgggcg atcggtcagc cggatgctcg
    11581 acgtgtagag cggaaactcg accggctccg agacgagcag gtcgaattgc cggtcgagct
    11641 cgatcgactg cccaggctcg gcgctgcctg ggacgaggca gagggccgcc ggtggatcgg
    11701 cttcgacgcc aatgtaatac gtccgcgcca gcgacgcggc gattctaacg ccttcgccgc
    11761 ggcggaccat gccgtaataa gccgccccac gagccaccgc cagatcgagg cggtcgttgg
    11821 ccagcacggc cggcttccag tcgtcgccgc caaaccaact ggagagggag tcgatcaacc
    11881 gctcctgcag aacgcccgac tcgaacacgc cgccattgaa cagcaacaca tccggccggg
    11941 cgggatcgac ttcagctgct gaaccctgaa ccccgaaccc tgaaccctcc tcgcccgcaa
    12001 gccgatgcgc cgtcaggaac gcggccagat accgcgtgat cgccgggtcg gccgcgtaag
    12061 gcaggccgaa ttcttgaaag ccgctgcggc gggcagcggg cttatcgctc aggctggcgc
    12121 gcggcatgaa gccatcgacc agcagcttgt gcacttcgtc gcgcgtcact ctggcttgca
    12181 gcgccccgcc aatcagcttc gatccgctgc cggggattgt tacggtcagc tcccctgggc
    12241 cgctatcgga cagcagcagc tccttcacct ggcgagcctg gcggaggagc gtgtcccatt
    12301 gacggggctg aagcttcgct ctagctggcg agcgggaggc gtcagcctcc cgataattct
    12361 ccactaggtt cccgccaatc ggattatcgg gaggctcacg cctcccgctc gccggtcgga
    12421 gcagctcttg ctcgagatat tgcgccaggg ccaaatccat attgtcgccg ccaagcaata
    12481 agtgatcgcc gacagcgacg cggtgaaact gaaccctggc gccatcggtc tcatcggtcg
    12541 catttgtccc atcgctcgcc ccagtgcgcc gcacccggat cagcgtgaaa tccgtcgtac
    12601 cgccgccgat gtcgcagacg aggatcttct gccccggagc gacgagcttt tcccagttgt
    12661 cctggtgctt gtaaatccag gcatagaacg ccgcctgcgg ctcttcgatg agtacgacgc
    12721 gggccagctc cgcgcgggct gcggcctgca ccgtcaattc gcgggcgatc tcgtcgaacg
    12781 atgccggcag cgtcagtacg atgtcctgtc gctccagcgg ctccgccttg aaccgctcgt
    12841 tccacgcccc gcggatgtgc gccaaatacc ggctgctggc ttcgaccggc gaaatccgct
    12901 ccacgtcggc agccgcatgc cagggcagca gctcggctgt gcgatcgacg ccagtatggc
    12961 agagccagct tttggccgac gagatcaatc ggccgggtgt ggccgcaccg taatcgcggg
    13021 ccataaagcc aacgacgtag gcggcgtttg gcgtttcgtg ttttgtgctt ggttcttcgg
    13081 ttctttggtt cttcggttct tccccctgcg gtggctgata gtggaagctc ggcagtgtgt
    13141 cgcgggcttc gacctgtccc ggggcgacga gttgcgggat ttccagtacc cgcacgcgcc
    13201 acggctgttc cgccgtatcc acgtagcaaa ccgcggaatt cgtggtgccc aggtcgatgc
    13261 cgacgacata gcggctgggc aagtcttcag gcagcaattc ggcgggcata gtttttttct
    13321 agcgacgagc tgccagcttg tcgcccgcga ccaatggtcg cggcttggga tcaggcgtct
    13381 gcgtcctttc gcacgctgaa ttccagcttc cagcggttgg gcgatttggt gctctggcac
    13441 cagagctcga agacgcccag ctcggtgagc tgcgactgga agcggacggg aacgaagtcg
    13501 tcgtcgatgg cttcgtcgcg cggaaggact gcttcgagcg agtcggtctc ggccagctct
    13561 tcgtcggtcc aggcgggcaa caaatcaccg ggctggtcct gcttgcgaac ggccgagctg
    13621 aagaagcgga aggtggcgct ttcaccaacg accaggccga tttcgctgga aggaacgtcg
    13681 atgctggtgc cttcttccag gccgaagggc acgacgcaca gggcccgcag cggccgcggt
    13741 gcgccgggaa tcgccaggcc gctggtttcg atgccgacgt agtacgagcg ggccgttccg
    13801 ccccgaatcc gcacgccgcc gtgctgcttg gcccagccgt aataggccgc accgcgggcc
    13861 accgcgtgat cgaggtcctg ctcgcccgcc agcgttctcg gaggagcatc gccggtccaa
    13921 tcgctgagca cgtcgagcag ccgactgcga aaggcatcgg ccttgaacac gcccccgtta
    13981 aacagtacgt gcgcgggccg gatcggctcg ccagccttgc cgtgggccgc caggaacgcc
    14041 gccaggtgcc gcgtgacggc ggcgtcggac tcgaacggca ggccaatttc ctgaaatccc
    14101 gaggcgcggc ggcggcccgg gcgatcggtg atctggcact tgggaaaaaa gccctccagc
    14161 agcagctcgg ctgcggcatc gcgctcaatc tcgaccttga ccgtgccgcc gatcagcttg
    14221 ctgccgcggc cgaggacgga gagcgggtgt ttcttcggcc cgccgccggt cagcagcgcc
    14281 tctttggcgg tgcggcactg atgccagagc gagatcgatt gccacggatc gagcttgacc
    14341 cctttctcgc caaacttgcc ggcgacgaag taagccaggg cgaggtccat gttgtcgccg
    14401 ccgacgagca agtgctcgcc gactgcgagg cgccgcagcg tcagctcgcc accctcctga
    14461 tcgactccga ccagcgtcag gtcggtggtg ccgccgccca cgtcgcagac caggagcgtt
    14521 tcgcccaccc tcagcagcct tcgccacttc tctccggctg ccgtcagcca ggcatagacc
    14581 gcagcctgcg gctcttcgag caggatcagg cttgcgggca agccggccgt cagcgccgcc
    14641 tcgcgcgtca gctcacgggc cgccgcatcg aacgacgccg gcaccgtcaa cacgaccagc
    14701 tgctcggcca ccggtgcctc gggatgagcg tgctcccacg ccgcgatcag atgctccaag
    14761 taccgccgcg aagccgtcac cggcgagacc ttgggcacct cggccggcgc ttgccagggc
    14821 aggatcgact gatggcgatc gaccttgctg tgcgccaacc acgacttcgc gcccgcgacc
    14881 gttcgctgcg ggctctcggc cgactgccgg cgggccaatt cgccaaccac gaagttgttg
    14941 cctttgcacc agggcagttc gaacgcgccg cccttggctt cgtgctccgg tgcgaggtac
    15001 agaaacgacg gcagcgccgt gcggctctcg accacccccg gcgcaaatag ctgcgggatc
    15061 ggcagcaact caactcgcgg ctgctcctcg ctcagggagg cacaggcgac gacgctgttc
    15121 gtcgtgccca gatcgatgcc gatgacatac ttggcaggca tgccgtctat gtttaccagc
    15181 cgcggcgaaa cgtgagtagg acaggcgctg ctacagccgc ggatcgaccg gctcgctttc
    15241 gagggcgagc acgccaaaga cgcactcatg cacgcggcgg agcggctcgc gtccgacgaa
    15301 gcgttcgagc gcctcgatcc ccagggcgaa ctcgcgcgag gccagcgagc gcttggcaag
    15361 ggtcgagcgc tttcgcaatc gggcgagatg ctcgctcagc gtgtattcgg gcccgtagat
    15421 gatccgcagg tattcccggc cgcggcattt catcgccggc tggaccagtc ccttgggtcc
    15481 ggcggcgatc cactcgcgcg gcttgacgac cattccttcg ccgcccgcgg tcgttagctc
    15541 ttcccaccat gtcgttgcgg ctgtagtgct ggcagcgtcg tgcagatcga ccagcagatg
    15601 ccgagtcgcc tggatcagtg gaaccgcggc gagctcgctc aaagtctgca tgtgccacag
    15661 gtggtcgtgg tgggtgaaga cttgcccttc ggccgccagc agatggaacg gtgccaggcg
    15721 caagtcggcc gcggagcgga ccggccagca gtagcggccg taagccgcgc gatagagcga
    15781 cgcacaggcg aggcgctcct ggagctgccc agcgatcggc tggtggctcg ccaatagcgc
    15841 cgccgcctcg cgcaagctcg cttcgccggc ggcggcgacc ggggcgtact ggtgctgcag
    15901 cagttcctgg gccttggcag accagggcat cagctcgcaa tcgaggcaga accagtcgct
    15961 ctgaaaacgc tcccaccagc cggcggccgt cattgccgac cagagcgcgt cgagcatgtc
    16021 cgattccagc tgcggttcgt cgaagaagcg acggccggtc cgcgtcacga tgatgccgcg
    16081 gccctcgtcc gtaacgccaa accgcttctg ggccgcggcc tcatcgcggg ccagcacgac
    16141 gaccgctcgc gagcccatgt gtttttcctg gcagaccaca tgctggatgc ctgcgccgcg
    16201 gaagtagtcg aagacctggt cgggatgctc caggagctcc ccttccggca ccgtctcgca
    16261 cggcgacatc gtcggcggca gatagatcag ccacttggga ttgaccgcaa accggctcat
    16321 gacctcgagg gccgcggcgc tttgttcggc gcggatcgtc actcggccag ccagccgcgt
    16381 ctcgatcagc cgcttgccca ggcagtcctc gatgtcgagc aggtcgtcga gctgctgctg
    16441 cgccgttagc gcgctgggag cgggcaggaa cggccgggcc ggttcgcagt agatccgccg
    16501 cgccgggatg gaaacgagct cgcgctccgg gtagcggagg gccgtgagtt tgccgccgaa
    16561 gacgcagccg gagtcgatgt tgatggtgtg gttgagccat tccggctccg ggaccggcgt
    16621 atggccatag acgaccatcg cctggccgcg atactcggcg gcccagttca gccgcaccgg
    16681 gagcccgaac tcgtcggttt cgccggtcgt ttcgccgtag agggcgaact cgcggacgcg
    16741 ccgcgaggtg cggccctggt attcggcctt catcccggcg tgggccacga ccagccggcc
    16801 gccgtcgaag acgtagtggc tgaccagcga gtcgaggaag gccgccgtct gggccttcca
    16861 ggcttcgcgg ctatccggtg gcagcgcctc gatctcggcc agggtttgct ccagtccgtg
    16921 cgtcacgtgg acttggcggc cctgcagatg cttgagcagc ttcatgtcgt gattgccggg
    16981 cacgcaccag gccgctccgc ttgtgaccat cgcccgcacg aggtccagca cggcgacgac
    17041 gcgcgggccg cgatcgacca ggtcgccgac aaagatcgct tggcggcccg cgggatgcgc
    17101 ccagctactg ccgctggcgg atgaggaata acccagccgc gtcagcagct cctcgagctc
    17161 gtccgcgcag ccgtgaacgt cgccgataat gtcgaatggc ccgcgttcat gccgcatgtc
    17221 gttccagagc ttctcccgct cgatcgtcgc cgcgtcgatt tgcgccggat cgttgaacgt
    17281 aaagacgtgg cgaaagccct cgcgggccag ctgcttgagc gagcggcgga gctggcgaac
    17341 ctgattgcga atcacgtggg ggccgaaggt gcggtccgcc cggccgctgt tgcgcgacgc
    17401 gcacagctcc tcgcggaggt tgaagacaat tgccacgggc aagacgtcgt gcgatttcgc
    17461 cagcgagagc agcggctgcc gcgcttcggt ctgcacgttg gtggcgtcga tgaccgtcag
    17521 cttgcctgcg gccagccgct tgctcgcgat cgcgtgcaga atctcgaacg ccgcggctgt
    17581 cgccgcctgg tcgttttcgt cgtcggaaac gagcccgcgg cagaaatcgg acgacagcac
    17641 ctcggttgct ttgaagtgct tgcgcgcgaa cgtgctcttg ccggcgcccg aagggccgat
    17701 gaggacgacc aggcaaagct cgggaatggc aatcttcatc gctgtcgatc aggtcgaagt
    17761 gaacgtcgcc atttgcgtcg gcgatccgtg atgcgaatcg gccgggccga ttccgctcag
    17821 ctgcacgccg tagccgaatt gcgccgcgac tcgctcggac caggaccgaa actcgtcccg
    17881 cgtccattcg aagcggtggt ccttgtgccg cagcttgccc ggcggcatcc cggggaagag
    17941 gacgttgtat tcgcggttgg gcgtcgtcac gacgacggtt tttggccgcg cgcactcgaa
    18001 gaccgcccgc tccagcgccc gcagccgggg cggatcgaga tgctcgatca cctcgaccag
    18061 cgcggccgcg tcgtagcccg ccagccgccg gtcgcgatac gtcagcgagc catgcagtaa
    18121 tttgattcgc tgagccttag ccgccggcag gcggtcgagc ttcagccgcc gctcggcagc
    18181 cgccagggca cggtgcgaca cgtcgagccc gacgatctcg ctgaattgcg gctcctccag
    18241 cagcttccgc aggaggttgc cggtgctgca acccaggtcg aggacgcgct gcgccccgct
    18301 ttgtacgaga gccgccacga cggccagcag ccgctgctcg ttcagccgca gcggctgctc
    18361 gacgagctcc tcggccgcgg tcttgtcggc ggcttcctct tcgtcttcca cgggctcgtc
    18421 agcgggcagg cgagccagcg cggcccgcgt aagcggtgct cggtagcgca ggtatcgctg
    18481 ggcgatctcc tcgcgccgtg gatggcgggc gagccactcc tc
//
LOCUS       DASRJB010000744         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1340873, whole genome shotgun sequence.
ACCESSION   DASRJB010000744 DASRJB010000000
VERSION     DASRJB010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340873"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFB80_19615"
     CDS             complement(<1..273)
                     /locus_tag="VFB80_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN36052.1"
                     /translation="MTKPLQGVLPIVQTPFLDSGEIDFAALRGEVDWALAQGSDGLGT
                     GMVSELLKLTADERRDLAQRLVEFTAGRCPVFMGVGAESTRQALAYA"
     gene            413..1795
                     /locus_tag="VFB80_19620"
     CDS             413..1795
                     /locus_tag="VFB80_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN36053.1"
                     /translation="MRRFFSFAIAAYIVASCSLASARPNILVIVGDDMGYGDIGVHGC
                     KDIPTPHLDSLAKNGVRCTSGYVSGPYCSPTRAGLMTGRYQQRFGHEFNPGPPPEGAE
                     IGLPLTETTFADRMKAAGYTTGMVGKWHLGSERKFHPLSRGFQEYFGFLGGAHPYFLA
                     NGDQPNNPVLRGFESANEKEYLTEAFAREAMAFIDKHAKEPWFLYLTFNAVHTPMHAT
                     DKYLARFKDVPDEQRRIYCAMMSAMDDAIGQVLKKLDENKLTENTLVFFVSDNGGPPV
                     NKSSNGPLRGNKAQTWEGGIRVPYLVQWKGTLPAGKTYDQPVIQLDIQPTALAAAGVE
                     AKDAKFDGVNLLPHLKGQAGSPPHETLYWRFGDQMAIRHGNYKLVHGRGAEQRMLFDL
                     AADIGERNDLTTAKPDVVKDLTARYDAWNATLMEPRWGQPNRALGTFKSAEEKAKNKA
                     KRAAAKKGKE"
     gene            1910..3418
                     /locus_tag="VFB80_19625"
     CDS             1910..3418
                     /locus_tag="VFB80_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN36054.1"
                     /translation="MKHTLPFLLSIGLALAASAQEKPKLNVLYICSDDLNNSLSCYGH
                     PLVKSPRIDELAQRGVRFDRAYCQYPLCNPSRSSFLTGLRPDQTGVLENATQFRQNIP
                     DVVTLPQLFQKAGYFVARVGKLYHYGVPGQIGTDGLDDPPSWMAKFNPRGRDKDDEPK
                     IFTLTPGPPPQFGGTPSWLAAEGQDREQTDAIGAEMTIKLLEQNKDQPFFIACGFYRP
                     HTPYVAPKKYFELYPPEKAGVAEQKPGDLDDIPAPALVSRRNDMDERLQREARQAYFA
                     SITFMDQQVGKLLDAIDRLKLADKTVIVFHSDHGYHLGEHGCLWQKQSLFEESARVPL
                     IIATPDSKAAGKGTAAIAELIDVYPTVAELCGLTPPANLPGKTLVPQLTDPSLPGKGF
                     ALTQVRRAGGGGGGGGKAKKAKAAADAKTTPAAAAPVAQQPKAKAAGGQFPGYSLRTD
                     RYRYTEWDGGARGVELYDQQKDPLEYTNLAQDPAHAETVKQLSAKLKELTAK"
     gene            3431..4000
                     /locus_tag="VFB80_19630"
     CDS             3431..4000
                     /locus_tag="VFB80_19630"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN36055.1"
                     /translation="MASATELDAIGAAVIEGALATLRANKGWADKAIAQVSDEQLHIA
                     LDANTNSIAVIMKHVAGNLLSRWTDFLTSDGEKPWRNRDDEFVDTFGTREELLAYWDS
                     GWQRLLETLAGLTPADLLKTVTIRGEPHTVPLALQRSLAHTCYHVGQIIQVARILAGD
                     HWTTITIPRGQSSKFNQRVWGTATYRPAS"
     gene            4127..>4413
                     /locus_tag="VFB80_19635"
     CDS             4127..>4413
                     /locus_tag="VFB80_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36056.1"
                     /translation="METTLHRQLKALYAGPEARVEERLEGFRIDAVRSGQLVEIQHGS
                     LGAIRDKIARLLKSHAVLVVKPLVVRKTLVKLSKRGGRELSRRQSPKQGSLL"
BASE COUNT          883 a         1547 c         1317 g          666 t
ORIGIN      
        1 cgcgtacgcg agcgcctgcc gcgtgctttc ggccccgacg cccataaaca ctgggcatcg
       61 acccgcggtg aattccacca gccgctgtgc caggtcgcgc cgctcgtccg ccgtcagctt
      121 gagcagctcc gaaaccatcc ccgtgcccag gccgtcgctg ccctgtgcca gcgcccaatc
      181 gacctcgccg cggagggctg caaagtcgat ctcgcccgag tcgagaaatg gcgtctgcac
      241 gatcggcagc acgccttgca gtggcttggt catggacggg attatagctg cctcaccgca
      301 gcgaaagaaa atcgcccccg ctgcgcagat tttcgccgcg ccgcgcatct atactgatcc
      361 gcaaatcgct cgactgcggt cgggtcctct ccttctagcg aaccggtgtt ccatgcgacg
      421 ctttttctcg ttcgccattg cagcgtacat cgtggcatcc tgctcgctgg cgtcggccag
      481 gcccaatatt ctcgtcatcg tcggcgacga catgggctat ggcgacatcg gcgttcacgg
      541 ctgcaaggac attccgacgc cgcacctcga ttcgctggcg aaaaacggcg tccggtgcac
      601 cagcggctac gtgtcggggc cgtattgcag cccgacgcgg gccggcctga tgaccggccg
      661 ctaccagcag cgttttggcc acgagtttaa ccccggcccg ccgccggaag gggccgagat
      721 cggcctgccg ctgaccgaga cgacgttcgc cgaccgaatg aaagccgccg ggtacacgac
      781 cggcatggtc ggcaagtggc acctgggcag cgagcggaag ttccatccgc tcagccgcgg
      841 cttccaggaa tattttggct ttctgggcgg agcccaccct tatttcctgg ccaatggcga
      901 tcagccgaac aacccggtgc ttcgcggctt tgaatcggcg aatgaaaagg agtacctgac
      961 cgaggctttc gcccgcgagg cgatggcgtt catcgacaaa cacgccaagg agccctggtt
     1021 cctctatctg acgttcaacg cggtgcacac gccgatgcac gcgaccgaca agtatctcgc
     1081 ccggttcaag gacgtgcccg acgagcagcg gcggatttac tgcgcgatga tgtcggcgat
     1141 ggacgacgcc atcgggcaag tgctgaagaa gctcgacgag aacaagctga ccgagaacac
     1201 gctggtcttc ttcgtcagcg acaacggcgg cccgccggtc aacaagagca gcaacggccc
     1261 cttgcgcggc aacaaggccc agacgtggga aggcggcatc cgcgtgccct acctcgtgca
     1321 gtggaagggc acgctgccgg ccggcaagac gtacgatcag ccggtgatcc agctcgacat
     1381 tcagccgacg gcgctggcgg cggcgggcgt ggaggccaag gacgccaagt ttgacggcgt
     1441 gaacctgctg ccgcacctga agggccaggc cggctcgccg ccgcacgaga cgctctattg
     1501 gcgattcggc gaccagatgg cgatccgcca cggcaactac aagctggtgc acggccgcgg
     1561 cgccgagcaa cggatgctct tcgacctggc cgccgacatc ggcgaaagga acgatctgac
     1621 aactgccaag cccgacgtcg tcaaagacct caccgcccgc tacgacgcct ggaacgccac
     1681 cttgatggaa ccccgctggg gccagcccaa ccgcgccctt ggcacgttca agtccgccga
     1741 agagaaagcc aagaacaagg ccaaacgggc ggcagcgaag aaggggaagg agtgacggag
     1801 agatggagtg agtgagggac gtgtgactct gatacttcct acttggtacg cctccgacta
     1861 gttatcccaa acacgaaaca caaaacacta aaaacgaacc agacacgcca tgaaacacac
     1921 gcttcccttc ttgctttcca tcggcctcgc tctcgccgcc agcgcccaag aaaagcccaa
     1981 actaaacgtc ctctacatct gctccgacga cctcaacaac tcgctttcgt gctacggcca
     2041 tccgctggtc aaatcgccgc ggatcgatga gctggcccag cggggcgtgc ggttcgaccg
     2101 tgcgtattgc caatatccgc tctgcaatcc cagtcgctca tcgttcctca cgggcctgcg
     2161 gccggatcag acgggcgtgc tggaaaacgc gacgcagttc cgccagaaca tccccgacgt
     2221 tgtcacgttg ccgcagctct ttcagaaggc cggttatttc gtggcccgcg tgggcaagct
     2281 ctaccactac ggcgtgcccg gacagatcgg caccgacggc ctggacgacc caccgtcatg
     2341 gatggccaag ttcaatccac gtggccgcga caaggacgac gagcccaaga ttttcacgct
     2401 cacgcccgga ccgccgccgc agttcggcgg cacgccgagc tggctcgccg ccgagggcca
     2461 ggaccgcgag cagaccgacg ccatcggcgc tgaaatgacg atcaagctgc tggagcagaa
     2521 caaggaccag ccgttcttca tcgcctgcgg cttctaccgc ccgcacacgc cctacgtcgc
     2581 ccccaagaag tactttgagc tctacccgcc ggaaaaagcc ggcgtagccg agcaaaagcc
     2641 cggcgatctg gacgacatcc ccgcgcccgc gctcgtctcg cggcgaaacg acatggacga
     2701 gcgtctgcag cgcgaggccc gccaggccta tttcgcctcg atcaccttca tggaccagca
     2761 ggtcggcaag ctgctcgacg cgatcgaccg cctcaagctg gccgacaaaa ccgtgattgt
     2821 ctttcacagc gaccacggct atcacctggg cgagcacggc tgcctctggc agaagcaaag
     2881 cctgttcgaa gaatcggccc gcgtgccgct gatcatcgcc actccggaca gcaaggccgc
     2941 cggcaaaggt accgccgcga tcgccgagct gatcgacgtc tatccgaccg tcgccgaact
     3001 ctgcggcctg acgccgccgg ccaacctgcc cggcaagacc ctcgtcccgc agctcaccga
     3061 tccttcgctc cccggcaagg gttttgcgct cacgcaagtc cgccgcgccg gcggcggagg
     3121 aggaggcggc ggcaaagcca agaaggccaa agccgcggcc gatgcaaaaa ccacgccagc
     3181 tgccgccgct ccggtcgccc agcagcccaa ggccaaagcc gccggcggcc agttccccgg
     3241 ctacagcctc cgtacggacc gctaccgcta caccgaatgg gacggcggcg cgcgaggcgt
     3301 cgagctctac gaccagcaga aggacccgct cgaatacacc aacctcgccc aggaccccgc
     3361 ccacgctgag accgtcaagc agctcagcgc caagctgaaa gagctgaccg caaagtaggg
     3421 aacgccctct gtggcctccg cgaccgaact ggacgcgatc ggagccgccg tcatcgaagg
     3481 cgcgctggcc acgctccgcg ccaacaaggg ctgggccgat aaggcgatcg ctcaggtttc
     3541 ggacgagcag ctgcacatcg ccctggacgc caacaccaac agcatcgcgg tcatcatgaa
     3601 gcacgtcgcg ggcaacctgc tctcccgctg gaccgacttt ctgacgagcg acggcgaaaa
     3661 gccctggcgc aaccgcgacg atgaattcgt cgataccttc ggcacccgcg aggaactgct
     3721 ggcctattgg gactccggct ggcagcggct gctggagacg ttggccggcc tgacgcctgc
     3781 cgacctctta aagactgtca cgatccgcgg cgagccgcac acggttccgc tggccctgca
     3841 gcgttcgctc gctcacactt gctaccacgt cggccagatc atccaggtcg cccgcatcct
     3901 cgccggcgac cactggacca ccatcaccat tccccgcggc caatcctcca agttcaacca
     3961 gcgcgtctgg gggaccgcca cctaccggcc ggcgtcgtga gccaagatcc agggctggcg
     4021 ggggccaccc gtagctcagc gcggaattga tgttgccacg cggcagcgaa gccggcacac
     4081 tccaagcgcc gcccgggatg acgccacgcg gagtgtgccc gctaccttgg aaaccaccct
     4141 ccaccgccag ttaaaggccc tctacgccgg gccggaggcc cgcgtcgaag agcggttgga
     4201 gggtttccgg atcgatgcgg tccgatccgg gcagctcgtc gaaatccagc acggcagcct
     4261 gggcgcaatc cgcgataaga ttgcccggct gctgaaatcg cacgcagtcc tggtggtcaa
     4321 gccgctcgtc gtgcgcaaga cactcgtcaa gctgagcaag cgcggcggcc gcgagctctc
     4381 gcggcgtcaa agccccaagc aaggcagcct gct
//
LOCUS       DASRJB010000745        16034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2752098, whole genome shotgun sequence.
ACCESSION   DASRJB010000745 DASRJB010000000
VERSION     DASRJB010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752098"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VFB80_19640"
     CDS             complement(<1..2078)
                     /locus_tag="VFB80_19640"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36057.1"
                     /translation="MTTVSLRVPLAIIGLAIGLLPGLAGAVGPEPTPEQIKFFEEKVR
                     PILATNCYKCHGSEQQKGQLRLDLREMALAGGEDGPVIVPGQPEKSLLVEAIKWESFE
                     MPPSGKLSESQIATLTEWIRLGAPMPKDHGGGSGIALRKNRGVITDEDRQWWAFRPIG
                     RPPVPAIQTVPAIQNSEFRTQNPIDSFVAAGLMQNGLSPAPEADRRTLIRRLTFDLTG
                     LPPTPEAIDEFLADQRPDAYERLTDRLLASPQHGEQWARHWLDLVRYAESDGYKQDAY
                     RPEAWRYRDYVVRSLNADKPYSRFVLEQLAGDEACPNDPDALIGTMYLRLGIYEYNQR
                     DVRNQWSIILNDLTDVTADVMLGMGLSCARCHDHKFDPILQKDYYRLQAFFAPILWRE
                     NVPAAREDEIAEYQRQLAAWEEATADVRRQIAEIEDPIRQAAMRTTFVKFTDDLLEML
                     EKPASERTPLESQLAYLIHYQVTDGEGKADLTGKLKGDKKERWQKLQEELAAYDHLRP
                     RPIAFIPSVTDVGPVAPEVTIPGRRDAEPVDPGPLSVLESASGGREAPGNVAFRPGES
                     DRKADASRSPGTTGRRTALAQWIASPDNPLPSRVLANRLWQHHFGRGLCESPSDFGRL
                     GEPPSHPELLDWLAGELLDNDWSMKRLHRLIVTSAAYRQASHGPEVAAAAAKDPANKW
                     LARMTVRRLSA"
     gene            complement(2235..4370)
                     /locus_tag="VFB80_19645"
     CDS             complement(2235..4370)
                     /locus_tag="VFB80_19645"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HZN36058.1"
                     /translation="MRFLGRFGASVCRPRNLFCIALVCLSIWAVATNRIPLGPRPSSA
                     QVQLVAGLQAPTANDRMVTRIVTTLMRREHLSKHALDDEISRRGLDLFIKSLDGMKLY
                     FNQSDIDEFNQKRNDLDDMVQAGDVSFAYTVFNRLLTRVDERVKLVDELLAGDFDFTA
                     DEILITDPDKLSYASTPDETKARWIKRLKYDVLVLKADKANKEPIKERLKRRYSSFAK
                     RMHQTDSNELLEMYLTAITSSFDPHSNYMAPVSAENFTLVMGLQLEGIGAQLRVTDGY
                     TIIDKLIAGGAADKQGQLKAGDRIVSVKQDNEEKAVDVGEMKLNDVVKMIRGRAGTVV
                     HLGVISPNSAEVKTIKITRAKIELKDQEARGQVFEEGKKADGSPFKLGVINLPSFYMD
                     MRGAREGEENYKSCSRDVLKIINDFNKQGVDALVLDLRKNGGGSLTEAISLTGLFIES
                     GPVLQVKDPDGRVQQYDDTDRGIAWKGPLVVMISKFSASASEILAGAIQDYHRGIVVG
                     DSSTHGKGTVQTMMDLGPQMFRIANPPDLGSLKLTVQQFYRPNGDSTQQRGVLADVLL
                     PSLTDHMDVSEADLDYPVAFDKVPTTQFHRFDSVTPNIVSTLKELSGGRMEKSTDFAK
                     LQKNIKRYEEQKAKKEVPLNEQKFLARRAELDADDEEEKIAEDQANGNAEVFKRTYYE
                     NEVLAITLDYLRLLGKDKVANAGKTAAPN"
     gene            complement(4502..5416)
                     /locus_tag="VFB80_19650"
     CDS             complement(4502..5416)
                     /locus_tag="VFB80_19650"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HZN36059.1"
                     /translation="MNARDGIRPPAGLLLIGHGTRDEVGTRQFLDLAAAVARQVNGRV
                     GQASPRAQTHAELAPRPRGSAAAAALTHPTVVRPSFLELQQPAIEDAIKDLAEVGVEH
                     VVAVPLLLFAAGHAKRDIPERLAAAAEQYGITTTQTAAFGCEPAILELSRQRMREAVT
                     GRLHVPAEETCLLLVGRGSRDESATAQMQEFAQLRQQAEGGIPTEIAFLAMARPSLDE
                     QLPRIAVSGFRRVIVQPHLLFQGELASSLARQVQSVAQNSRSEWLLTPLLADLAKEVG
                     IGTKILADVICERYRAATIRVVAPAGED"
     gene            complement(5413..7461)
                     /locus_tag="VFB80_19655"
     CDS             complement(5413..7461)
                     /locus_tag="VFB80_19655"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN36060.1"
                     /translation="MRSRFLLLALLLAGLGCFSVRAAENAAESEARLRDNVTYLASDA
                     LEGRGVGTEGLNKAADYLARKFAELGLKTDLFDGTPFQNFEIVVSAEMGPADKNRLAL
                     AGPERRIELKLGENFTPLATGGTARFEAPLVFAGYGITAKNLKQGDKTISYDDYAGID
                     AKGKVVILLRKEPQQQDEKSPFNGTRTTQHALFGTKIKNALDHGAAAVIIVNDGLELK
                     SRREQDTKLLKASLDTLAGLRDKLAALSPVDADFAKLSSSVAKSAAEATELAQQLAAS
                     PDVLLPFNGAGDTSTQRKLPVLFCTRGAIDPLLKSALDKDLATLEQEIDRDLAPHSAV
                     LAGWQAIGESEVHEKKAAIKNVVAVLEGQGPLADETIVVGAHYDHLGRGGAGSFAPWT
                     PDIHNGADDNGSGTAALLEIAHRLATTAGKPRRRIVFIAFTGEERGLLGSAHYCRQPR
                     FPLDKTIAMFNLDMVGRLKDEKLIVYGTGTAKEFDPLAERLSQQLGFKLTKHEGGFGP
                     SDHSSFYAKKIPVLHLFTGTHSDYHRPSDDSEKLNIAGMRRIADWVVEAVQATDAADA
                     RPSYIENKKFEQIADSQSSADPAAGDRPSFGSMPAYPNPVKDGVLLEAVMEGSAADKA
                     GIKAGDILIQLGEAKVTDLDAFQAALTSHKPGDKVKAKVRRGEKTIEAEVTLGRRRGP
                     "
     gene            7778..8914
                     /locus_tag="VFB80_19660"
     CDS             7778..8914
                     /locus_tag="VFB80_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36061.1"
                     /translation="MRILVAAALAAAWLALVGEARVAQAQPRAIRGGFGGPVPPGVAP
                     AASSDVKPPDKDEPIVEVPRGPVVELGPKHLRMHLLDGSVISGDLSISEVEVETSFGK
                     LVVPIDKIRSLRPGLDSNPKLLAHIEGLIKGLSDDDYKTREQAHKDLAAMGVKIRNEL
                     QRFAADENAEIKRHVGEILKELEEQSEQQADDDPSAVEQAWIRLDTLVTTEFTVTGKV
                     SPQAFQISSKYGPLTIQLGDIKEGERQTAVRESLRRNITIDGSNIAQRSFKSSGIRVQ
                     AGDKISIKADGSVVMSPWGSNAAASPDGAPNYGWYVPNQIPGGALVARIGDKGTVFKV
                     GRQSTFVAKNSGVLQLAIGMQAEYANEGYQYPGQYQVKLRVDPK"
     gene            complement(9347..9616)
                     /locus_tag="VFB80_19665"
     CDS             complement(9347..9616)
                     /locus_tag="VFB80_19665"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN36062.1"
                     /translation="MIRLAVLWAKCFHYFQLNREAFLTRYHQRSNVESTFSAVKRKFG
                     DSLRSKTDVAMKNETLAKFICQNVCCTIQEMHESGIDPTAWAASA"
     gene            complement(10494..11405)
                     /locus_tag="VFB80_19670"
     CDS             complement(10494..11405)
                     /locus_tag="VFB80_19670"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36063.1"
                     /translation="MQKPCFHLAAGRRWRDPRRRGFTLVELLVAIAIIGGLLAILLPA
                     VQRAREAARAARCKNSLKQIGLALHNYHDIYLRLPMGSSRADDGSWGFLLALMPQLDN
                     APAHNLVDFSNDCCAEIMQRQDTSPPRPDPASVFYPVLACTSDPHIRKLHTSGGGVSL
                     PCGRLYPASYLGVSGSESAFGGGTTSGDGLLYSLSRVRLAEVQDGTSNTMIVGERGLP
                     SDLVLGWVICGGTDGDQYLGAEFPPASGKNAPWTDGLAHGFWSWHPGGVHFVFADGSV
                     HLLANTIDPALYQALATRSGGEVVTTP"
     gene            complement(11489..11776)
                     /locus_tag="VFB80_19675"
     CDS             complement(11489..11776)
                     /locus_tag="VFB80_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36064.1"
                     /translation="MKRVRVLGLLLAVAAILAVPVSHLVWGAPPPGNKQLICHIDEDG
                     TGKVLEISVHAIPAHCAHHAGDYTSPPMGSPALVKGAACPRLAANAVRVCR"
     gene            complement(11894..13132)
                     /locus_tag="VFB80_19680"
     CDS             complement(11894..13132)
                     /locus_tag="VFB80_19680"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN36065.1"
                     /translation="MAAGTTRWSLVGLVLIGLGSAASGYLLVRSWDLMAGGAMGRLDV
                     CSTVFGRGCDATLQSASSWQLGIPLAGWGIVYYCTLAALLALSWWLGAAFRAEALLAA
                     LLLAALGAGISLLLAGLVLVGWAPHCPLCLIVQLANLALVPALKKASGLTVRELRELA
                     GGGCRYLLGRTAAAESSAPWKVVGFFAAALVGVVAYQWVFVETALRRSAASAGVTPEQ
                     ALREYQSAARQDLVIDATDPRLGPPDAPVQLVVFISFQCPACADLARSLRPLPEEFGP
                     RLCLAVKHFPLSSACNPALRVDKQPRACEAARAAVAAQAQGQFWPMHDALLQADLSAE
                     DALPRIAREAGLDLARFRAELAAPATAAKVQSDVELARRLGVAETPAVFLNGRRVQHF
                     SPEVLRFLIVHELAAQRPEH"
     gene            13288..14151
                     /locus_tag="VFB80_19685"
     CDS             13288..14151
                     /locus_tag="VFB80_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36066.1"
                     /translation="MSWRWICLGCWLMAGGSGCARWVTTKVEPPSQLPQVKAAADAVM
                     LDVAFVKQPAADVDSYDAIWAAADEQVLSTDLRRRLAANGLRAGVFGQDLPAPLRELL
                     DNPTAESQGQEADAEGGEGAGSRRHLQVRTGVWKKVYASQTQPLLAVLLHTGGSVHGH
                     QLTSAQCLFGLKTYPKGDGRVKLDLTPEIEHGELKPKFIRSEAQLVQSIGRDRLMLDE
                     LRIEALLSPGQWLLLSTTRDVKGLGENFFVDTPSGAVRRTLLLVRVAQTQLDDLFAPE
                     QTSAPLATPGD"
     gene            14313..15329
                     /gene="fba"
                     /locus_tag="VFB80_19690"
     CDS             14313..15329
                     /gene="fba"
                     /locus_tag="VFB80_19690"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650067.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZN36067.1"
                     /translation="MPLVPLRKVLDHAAANNYGVAAFNVNNMEQIQAIMEAAKETDSP
                     VIIQASRGARAYSQDNYLRHLMLAASELYPQIPVVMHQDHGNSPATCLSAIANGFTSV
                     MMDGSLKEDGKTPADYDYNVKVTSEVVKQAHAKNVSVEGELGCLGSLEHGEGEQEDGH
                     GAVGKLSREQLLTDPDQAEDFVKKTGVDALAVAIGTSHGAYKFTRKPTGEVLAMDRIV
                     AIHKRLPNCHLVMHGSSSVPQDLQDELRKYGGEMKQTWGVPVEEIQKGIQHGVRKINV
                     DTDCRIAITAAIRKVLEETPEKFDPRDYLKPAREAMKKVCIARMTSFGQAGNASKMRS
                     AGLI"
BASE COUNT         2808 a         5152 c         5183 g         2891 t
ORIGIN      
        1 gccgagagcc gccggacggt catccgcgcc agccatttat tggccggatc cttggcggcc
       61 gcggcagcga cctcggggcc atgcgacgcc tggcgatacg cggcggaggt cacgatgagc
      121 cggtgcaggc gcttcatgct ccagtcgtta tcgagcagct cgccggcgag ccagtcgagc
      181 agctccggat gtgacggcgg ctcaccgagg cggccgaagt cgctgggcga ttcgcacaag
      241 ccgcggccaa aatgatgctg ccagaggcga ttggccagca cgcgggaggg cagcggatta
      301 tcgggcgagg cgatccactg agcgagcgcg gtgcggcggc ccgtcgtacc cggcgagcgg
      361 gaggcgtcag ccttccgatc actctcgcct ggcctgaatg cgacattacc gggggcctca
      421 cggcccccgc tcgccgattc cagcacgctc agcggtccgg ggtcgacagg ctcggcgtcg
      481 cggcggccgg ggatggttac ttccggcgcg acggggccga cgtcggtgac cgagggaatg
      541 aacgcgatcg gccgcggccg caagtgatcg tacgccgcca gctcctcctg cagcttctgc
      601 caccgctcct tcttgtcgcc cttgagcttc ccggtcagat cggctttccc ttcaccgtcg
      661 gtcacttggt agtggatcag gtaagcgagc tgcgactcca ggggcgtccg ctcagacgcc
      721 ggtttctcga gcatctccag gaggtcgtcg gtgaatttga caaacgtcgt ccgcatcgcg
      781 gcttgccgga tcgggtcttc gatctcggcg atttgccgcc ggacgtcggc cgtcgcctcc
      841 tcccacgcgg ccagctgccg ctgatactcg gcgatctcgt cttcgcgagc cgcgggcacg
      901 ttctcgcgcc acaggatcgg cgcgaaaaag gcctgcaagc ggtagtaatc cttttgcagg
      961 atcgggtcga acttgtgatc gtggcagcgg gcacaggaga ggcccatccc gagcatcaca
     1021 tcggcggtca cgtcggtcag gtcgttcaag atgatcgacc actggttccg cacgtcgcgc
     1081 tggttgtatt catagatgcc cagccgcagg tacatcgtgc cgatcagcgc gtccggatca
     1141 ttggggcagg cctcgtcgcc ggccagctgc tccaggacga atcgcgagta cggcttgtcg
     1201 gcgttcaggg agcggacgac gtaatcgcga tagcgccacg cctcggggcg ataggcgtcc
     1261 tgcttgtagc cgtccgattc ggcgtagcgg accaggtcga gccagtgccg ggcccactgt
     1321 tcgccgtgct gcgggctggc cagcagtcga tcggtcaaac gttcgtaggc atcgggccgc
     1381 tgatcggcca ggaattcgtc gatcgcttcg ggcgtcggcg gcaggcccgt gaggtcgaac
     1441 gtcaaccggc ggatgagcgt gcggcgatca gcttcgggcg cgggagagag gccgttctgc
     1501 atcaagccgg cagcaacgaa cgagtcgatc ggattctgag ttctgaattc tgaattctga
     1561 attgccggca ctgtctgaat tgcaggcacg ggcggtctgc cgatgggccg aaacgcccac
     1621 cactggcggt cttcgtcggt gatgacgccg cgattcttgc ggagcgcaat gccgctgccg
     1681 ccgccgtggt ccttcggcat cggcgcgccc aggcgaatcc actcggtcag cgtggcgatc
     1741 tgcgactcgc tcagcttgcc gctgggaggc atctcgaacg attcccactt gatcgcctca
     1801 accagcaagc ttttttccgg ctggcccggc acgatcaccg ggccgtcttc gccgccggcc
     1861 agtgccattt cccgcaggtc caaccggagt tggcccttct gctgctcgga gccgtggcat
     1921 ttgtagcaat tggtggccag aatcggccgg actttttcct cgaagaactt gatctgctcg
     1981 ggggtcggct ccggcccaac tgccccggcc agccccggca gaagtccaat cgccagtccg
     2041 atgattgcta gcggtacgcg gagggagacg gtcgtcatac gcatcctcat cggcagcgcg
     2101 cgtggctcgt cctcggccag ctcccaatgc taaacccgcc aatcacttac gtcaacgagc
     2161 aggcagcgag agacgcatag cgcgcagccg gccgcccgac agtagtgttg ggcggcagcg
     2221 atgatcggcc gttgctagtt gggagcagcc gttttgccgg cgttggcgac cttgtccttg
     2281 ccgagcagcc gcaggtagtc gagcgtgatg gccaggacct cgttctcgta gtaggtccgc
     2341 ttgaagacct cggcgttgcc gttggcctga tcctcggcga tcttctcctc ctcgtcgtcg
     2401 gcgtcgagct cggcgcggcg ggcgaggaat ttctgctcgt tgaggggcac ttccttcttg
     2461 gccttttgct cctcgtagcg cttgatgttc ttctgcagct tggcgaagtc ggtcgatttc
     2521 tccatccgcc cgccggacag ttccttgagg gtgctgacga tgttcggcgt caccgaatcg
     2581 aagcggtgga actgcgtcgt ggggactttg tcgaaggcga ccggataatc caggtcggcc
     2641 tcgctgacgt ccatgtggtc ggtcagcgac gggaggagca cgtcggccaa aacgccccgc
     2701 tgctgcgtgc tgtcgccatt ggggcgatag aactgctgca cggtcagctt cagcgagccg
     2761 aggtccggcg ggttggcgat gcggaacatc tgcggcccca ggtccatcat cgtctggacc
     2821 gtgcctttgc cgtgcgtcga ggaatcgccc acgacgatgc cgcggtgata gtcctggatc
     2881 gcgccggcca gaatctcgct ggcgctggcg ctgaacttgc tgatcatcac caccagcggg
     2941 cccttccagg cgatgccgcg gtcggtgtcg tcatactgct ggacgcggcc gtcggggtcc
     3001 ttcacttgca ggaccgggcc gctctcgatg aacagtcccg tcagcgaaat cgcctccgtc
     3061 aggctgccgc cgccgttctt ccgcaggtca agcaccaggg catcgacgcc ctgcttgttg
     3121 aagtcgttga tgatcttcag cacgtcgcgc gagcagctct tgtagttctc ctcgccttcg
     3181 cgggcgcccc gcatgtccat gtagaagctg ggcaggttga tcacgccgag cttaaacggc
     3241 gagccgtcgg ccttcttgcc ttcctcgaag acctgaccgc gggcttcctg gtccttcagc
     3301 tcgatcttgg cccgcgtgat cttgatcgtc ttcacctcgg cgctgttcgg ggagatcacg
     3361 cccaggtgga ccaccgtgcc ggcgcggccg cggatcatct tcaccacgtc gttgagcttc
     3421 atttcgccga cgtcgacggc cttctcttcg ttgtcctgct tgacgctgac gatgcggtcg
     3481 cccgccttga gctggccctg cttgtcggcc gccccgccgg cgatcagctt gtcgatgatc
     3541 gtgtagccgt cggtcacgcg cagctgagcg ccgatgccct ccagctgcag gcccatcacc
     3601 agcgtgaagt tctcggccga gaccggggcc atgtagttgc tgtgcggatc gaagctgctg
     3661 gtaatcgcgg tcaggtacat ctccagtagc tcgttgctgt cggtctggtg catccgcttc
     3721 gcaaagctgg agtaccgccg cttcagccgt tccttgatcg gttccttgtt cgccttgtcg
     3781 gccttgagga ccagcacgtc gtatttcagc cgcttgatcc agcgggcctt cgtttcgtcc
     3841 ggcgtggagg cgtagctgag cttgtcggga tcggtgatca ggatctcgtc cgcggtgaag
     3901 tcgaagtcgc cggccagcaa ttcatcgacc agcttgaccc gctcatcgac ccgcgtgagc
     3961 aggcggttga agaccgtgta ggcaaagctc acgtcgcccg cctgcaccat gtcgtccagg
     4021 tcgttccgct tctggttgaa ctcgtcgatg tcggactgat tgaagtagag cttcatcccg
     4081 tccaggctct tgatgaacag gtcgagcccg cggcgcgaga tttcgtcgtc cagcgcgtgt
     4141 ttgctgaggt gctcgcggcg catcaaggtc gtcacgatcc gggtgaccat ccggtcgttg
     4201 gccgtcgggg cctggaggcc ggcgaccagc tgcacctggg ccgagctcgg ccgcgggccg
     4261 agagggatcc ggttggtcgc caccgcccag atactcaagc agacgagggc gatgcaaaag
     4321 aggttgcggg gccgacaaac cgacgcaccg aagcgaccta gaaagcgcat tccattcccc
     4381 ttaacgttag tccaacgaaa cctcgctccg aacgcaccgt ttcgaagccg taagctccta
     4441 gcgcccaaca ctttccgata atatgccccc gaccaggcgg aagcaagcaa acgggcgaat
     4501 tctagtcctc ccccgccgga gcgacaacac gaatagtcgc cgcgcggtat cgctcgcaga
     4561 ttacatccgc gagaatcttc gtcccgattc ccacttcctt tgccaaatcg gccaaaaggg
     4621 gcgtgagcag ccactcgcta cgcgaatttt gtgccacaga ctgcacctgc cgcgccagcg
     4681 aggaggccaa ctcaccctga aacagcaagt ggggctgcac aatcacgcgg cgaaagccac
     4741 tgacggcaat tcgtggcagt tgctcatcca gggaggggcg ggccattgcc aaaaaggcga
     4801 tctccgttgg gatgcctccc tctgcctgtt gccgcaactg ggcaaattct tgcatctggg
     4861 cggtggccga ttcgtctcgg ctgccgcgac cgaccagcag caggcaggtt tcctctgccg
     4921 gaacgtgtaa ccgcccggta acggcctcgc gcattcgctg gcgcgacagt tccaggatcg
     4981 ccggctcgca gccgaaggcg gccgtttgcg tggtcgtgat cccgtactgc tcggccgccg
     5041 ccgcgagtcg ttcgggaatg tcgcgcttcg catgcccggc ggcgaagagc agcagcggca
     5101 ccgccactac gtgctcgacg ccgacttcgg cgagatcctt gatcgcgtcc tcaatcgcag
     5161 gctgctgaag ctccaggaag gacggcctga caaccgtagg gtgggtcaag gccgccgcgg
     5221 ccgcagaccc acgcgggcgc ggcgcgagtt ccgcgtgggt ctgcgctcgc ggactcgctt
     5281 gacccaccct accattaacc tggcgggcga cggcggcggc cagatcgaga aattgccgcg
     5341 tgccaacttc gtcgcgagtg ccgtggccaa ttagcagcag gccggccggc ggccgaatgc
     5401 cgtcgcgcgc gttcatggcc cacggcggcg gccgagggtg acttcggcct cgattgtctt
     5461 ctcgccgcgg cggaccttgg ccttcacctt gtcgccgggc ttgtgggaag tcagagcggc
     5521 ctggaaggcg tcgagatcgg tcaccttggc ttcgccgagc tggatcagga tgtcgccggc
     5581 cttgatgccc gccttgtcgg cggcgctgcc ttccatcacg gcctccagca gcacgccgtc
     5641 cttcaccggg ttgggatagg cgggcatgct gccgaacgac gggcggtcgc cggcggccgg
     5701 atcggccgac gattgcgaat cggcgatctg ctcgaacttc ttgttctcga tgtagctggg
     5761 ccgggcatcg gcggcgtcgg tggcttgcac cgcttccacg acccagtcgg cgatccgccg
     5821 catgccggcg atgttcagct tctcgctgtc gtcgctcggt cggtggtaat cgctgtgcgt
     5881 gcctgtgaag aggtgcagca cgggaatctt cttggcatag aacgacgagt ggtcgctggg
     5941 accgaagccc ccctcgtgct tggtgagctt gaagcccagc tgctgcgaca gccgctcggc
     6001 cagcggatcg aattccttcg ccgtccccgt gccatagacg atcagcttct cgtccttcag
     6061 gcggccgacc atgtcgaggt tgaacatcgc gatcgtcttg tcgaggggaa atcgcggctg
     6121 tcggcaatag tgggcgctgc ccaggaggcc ccgctcctcg ccggtgaagg cgatgaacac
     6181 gatccgccgt cgcggcttcc cggccgtcgt ggccagccgg tgagcgattt cgagcagcgc
     6241 agccgtgccc gagccgttgt cgtcggcgcc gttgtggatg tcgggcgtcc agggtgcgaa
     6301 cgagccggcc ccgccgcggc cgaggtggtc gtaatgcgcg ccgacgacga tcgtctcgtc
     6361 ggccagcggg ccttgccctt ccagcaccgc caccacgttt ttgatcgcgg cctttttctc
     6421 gtggacttcg ctttccccaa tcgcctgcca gccggcgagc accgcgctgt gcggcgcaag
     6481 gtcgcgatcg atctcctgct cgagcgtcgc caggtcctta tccagggcgc tcttcaggag
     6541 cgggtcgatt gcgccgcgtg tgcagaacag cacgggcagc ttgcgctgcg tgctggtgtc
     6601 gcccgcgcca ttgaacggca gcagcacgtc cggactggcc gcgagctgct gggccagctc
     6661 ggtcgcttcg gcagcactct tggcgacgct ggaagacagc ttcgcgaaat ccgcatctac
     6721 gggcgacagc gctgccagct tgtcgcgcag accggccagc gtatcgagcg acgccttcag
     6781 cagcttcgtg tcctgctcgc ggcggctctt cagctccagg ccgtcgttga cgatgatcac
     6841 cgcggccgcc ccgtggtcga gggcgttctt gatcttcgtg ccgaagagtg cgtgctgcgt
     6901 cgtccgcgtg ccgttgaacg ggctcttctc gtcctgctgc tgcggctcct tgcggagcag
     6961 gatcacgact ttgcccttgg catcgatgcc cgcgtagtcg tcgtagctga tcgtcttgtc
     7021 gccctgcttg aggtttttgg ccgtgatgcc gtagccggca aagaccagcg gggcttcgaa
     7081 gcgagcggtg ccgccggtgg ccagcggcgt gaagttctcg ccgagcttga gttcgatgcg
     7141 ccgctcaggg ccggcgagcg cgaggcgatt tttatcggcc ggtcccattt ccgccgagac
     7201 gacgatctcg aagttctgaa acggcgtgcc gtcaaacagg tccgtcttca ggcccagctc
     7261 ggcgaacttc ctggccaggt aatcggccgc cttgttcagc ccctccgtgc cgacgccgcg
     7321 gccttctagg gcgtcggagg ccagataggt cacgttgtcg cgcaggcggg cctcggactc
     7381 cgcggcgttc tcggcggcgc ggacggaaaa acaacctagt ccagccagca gcagggccag
     7441 cagcaagaat cgggagcgca taggcgggaa gcgaagcggt ggtgaggcag gaatcgaaga
     7501 attcagtata acgggcctga cctcaaagct gcgacccgag gtcgcgctct ggatgtcacc
     7561 gaattcgcca gaaatcggga ttctgtgaac catccgatct ctggcgagtt tggctacgaa
     7621 tcgtacaggg cgaccactgg tcgcggcttg gctgccgctt cgtaacaaga acgtccgggc
     7681 gggccggttc gtcctacgcc tagtggaaaa gggtaggcta ggaagtgaca atagacggat
     7741 tgactgttcc cttatcgatc agcgactcgg tggcgtcatg agaatcctgg ttgcggcggc
     7801 tctggcggcg gcgtggttgg cactggtggg cgaggcgcgg gtcgcgcagg cccagccgcg
     7861 ggcgatccgc ggggggtttg gcgggccagt gccgccgggc gtggcgccgg ccgcctcgag
     7921 cgacgtcaag ccgccggaca aggatgagcc gatcgtggaa gtgccgcgtg ggccggtcgt
     7981 cgagctcggt cccaagcacc tccgcatgca cctgctcgac ggcagcgtca tcagcggcga
     8041 cctgtcgatc agcgaggttg aggtggaaac ctcgttcggc aagttggtgg tgccgatcga
     8101 caagattcgc agcctgcggc cgggtctgga cagcaacccc aagctgctgg cccacatcga
     8161 aggactgatc aagggtttga gcgacgacga ctacaagacc cgcgagcagg cccacaagga
     8221 cctggcggcg atgggcgtca agatccgtaa cgagctgcag cgatttgcgg ctgacgagaa
     8281 cgccgagatc aaaaggcacg tgggtgaaat cctgaaggag ctggaggagc agtccgagca
     8341 gcaggccgac gacgacccct ccgcggtcga gcaggcttgg attcgcctcg acacgctcgt
     8401 gacgacagag ttcacggtga cgggcaaggt ttcgccccag gcttttcaga tttcgagcaa
     8461 gtacgggccg ctgacgatcc agctgggcga catcaaggaa ggcgagcggc agaccgcggt
     8521 ccgcgagtcg ctccggcgga acatcacgat cgacggttcg aatatcgccc agcgttcgtt
     8581 caagagctca ggtatccgcg tgcaagccgg cgacaagatt tcgatcaagg ccgacggcag
     8641 cgtcgtcatg tccccctggg gcagcaacgc cgccgccagc cccgacggcg ctcccaacta
     8701 cggctggtac gtcccgaacc agattcccgg cggcgcgctc gtggcccgca tcggcgacaa
     8761 aggcacggtc ttcaaagtcg gccgccagtc caccttcgtc gccaagaact ctggcgtgct
     8821 ccagctggcc atcggcatgc aggccgagta cgccaacgaa ggctatcagt accccggcca
     8881 atatcaggta aaactgcgcg tcgatcccaa gtgatcgccg agttgcgatc gcttggactg
     8941 aatggaaagg cgtgcacggg atcgaacggc agcggcactc taccggcgac tgcatccacg
     9001 cttcgagcgc cgagcacacc tacagcccga gccgattcgc gaacccatgg cgaatcggct
     9061 ttgcccaaca agtcctaacc cgccttcact tgctgggcaa ccgccgattc tgcccaacaa
     9121 gttgtcattc tgggatgtag caggcaacga actttcggcc gtcgcatcgc gggcagcgct
     9181 tttcagggat ttggtccgca agccccggat ttctccaagc agagtatatg tcatcaaggc
     9241 agcaacctag acacagcttg attccgcacg cgcagcaagc ggctgttgcg ggcatttcca
     9301 agcagtgttc gcagggcagc tcgtccatgc cctgatttta cacgcgctag gcagatgccg
     9361 cccaagctgt cggatcaatc cccgattcgt gcatttcctg aatcgtgcaa cagacgttct
     9421 ggcaaatgaa cttcgccagc gtttcgtttt tcatcgccac gtccgttttg ctgcggagcg
     9481 agtcgccgaa cttccgcttg acggcgctga aggtcgattc cacgttgctc cgctggtggt
     9541 agcgagtcag gaacgcctcg cggttcaatt ggaagtagtg gaagcacttc gcccacaaga
     9601 ccgccagccg tatcaaccgc gttccgcttg aacgggacca gcgggtaaga gccggcagcg
     9661 tcgatgacgg ccagattgtt cctggtcgaa taagccaagt cggccacgac ctcgcgaacg
     9721 ttgaattgct gggacttgtt gggcagccgt cacctattgg gcaaagccat ggcgaatctg
     9781 aacattggta cgctatttgc agtatagttc ccttcggcgc ggcggactgc catttttggc
     9841 tgcccgcccg caaatgtcgc ccgctctctc gcaatttggt cgttggcaac tgtggcggaa
     9901 tcgttgccgt tccgcttcgt gactttatat aacgaaaact ccccgccggt cctcaaaggc
     9961 ttatgttcgt aatgcctgaa aaccgcctgg ccaaacgtcg gaagtcctcg cgtgccagcc
    10021 agttgcggcg cgagtggcag cgcaacgagg cgcgacgaga atctgtgctc gcggcagttt
    10081 tcgtaatgat cgccgacggc ccttaagggc agcaggcggg tccgcccccc cccaaaactg
    10141 atcgatcatg atacaaccgc gtgagcgcgc ttgacagctt ctcgcaatcg cgcgccggga
    10201 taatcgagcg tggaggggct gcgaattatt cgccactctc atttcgcacg taactgcgat
    10261 gccgcaaggc acttgcgacc ggcggccgtg aacttatcgc cagatcccca ggtattgagg
    10321 cagattggcg aataattctc ggcccaaagt gtggaaaaca ctggaacttt cgcaaaacga
    10381 ggccgaatta ttcgccaccg agccgcccga cccggtgaag aactcggggg gagcgcgcag
    10441 gtttagccca gaggtgccga cctgccaaaa tccaagcttt gactatgcac tagctagggc
    10501 gtggttacga cctcgccgcc ggagcgggtg gccagggcct ggtagagggc gggatcgatc
    10561 gtattggcca ggaggtggac gctgccgtcg gcaaatacga agtggacgcc gccggggtgc
    10621 cagctccaga agccgtgggc caggccatcg gtccacgggg cgtttttgcc ggaggcgggc
    10681 ggaaactcag cgcccaggta ttggtcgccg tcggtgccgc cgcagatcac ccagcccagc
    10741 acgaggtcgc tcggcaggcc gcgctcgccg acgatcatcg tattggacgt gccgtcctgc
    10801 acttcggcca ggcgaacccg gctcaaactg tagagcaggc cgtcgccgct ggtcgtcccg
    10861 ccgccgaatg cgctctcgct gccgctgacc ccgaggtagc tggcgggata gagcctgccg
    10921 cagggcagcg agacgccgcc gccgctggtg tgcagcttgc ggatgtgcgg gtcgctggtg
    10981 caggccagga ccggatagaa caccgaggcc gggtcgggac gcggcgggct ggtgtcttgc
    11041 cgttgcatga tctcggcgca gcagtcgttg ctgaaatcga ccaggttgtg cgccggagcg
    11101 ttgtcgagct gcggcatcag ggccagcagg aagccccagg agccgtcgtc ggcccgggag
    11161 ctgcccatcg gcaagcggag atagatgtcg tggtagttgt gcagcgccag tccgatctgc
    11221 ttcaggctgt tcttgcagcg cgcggcccgc gccgcctcgc gtgcccgctg caccgcgggc
    11281 agcaggatcg ccaggagccc gccgatgatc gcgatcgcga ccagcagctc cacgagcgta
    11341 aagccgcgtc gtcgcggatc gcgccagcgg cgacccgctg cgagatggaa gcacggtttt
    11401 tgcatgcgcc gcgggcagga gttgtggatc gtcgcgttcg cgcgtagccg cggctggcag
    11461 ccgcggctac tccgataacg tcaacgccct agcggcagac gcgaacggca ttggcagcaa
    11521 gccgcggaca cgccgcgccc ttgaccagcg cgggcgagcc catgggaggg ctcgtatagt
    11581 cgcccgcgtg atgggcgcag tgcgcgggaa tcgcgtggac cgagatctcg agcaccttac
    11641 ctgtcccatc ctcgtcaata tggcagatga gctgcttgtt tcccggcgga ggcgcacccc
    11701 acaccagatg cgagacaggc accgccagga tcgcggccac agccaacagc aatccaagaa
    11761 cgcggactcg tttcatgacc agcctccccc aatcaaaaga gaaatgaagc tgtcagaatc
    11821 caccagcccc gaatgtaagg gcctagcgcg gctcctgcaa gaattatccc aaagttttct
    11881 ttgggctctg ccatcagtgt tcaggccgct gggcggcaag ctcgtgaacg atcaggaaac
    11941 ggagcacttc cgggctgaag tgttgcactc gccgcccgtt gaggaagacg gcgggagtct
    12001 ccgccacgcc caaacgccgc gcgagttcaa cgtccgactg caccttggcc gcggtcgcag
    12061 gagccgccag ttcggctctg aatcgagcga ggtccaagcc agcctcgcgg gcgatccgcg
    12121 gcagggcgtc ttcggccgac aagtccgctt gcagcagcgc atcgtgcatc ggccagaact
    12181 gcccttgggc ctgcgccgcc acggcggctc gcgccgcctc gcacgcgcgc ggctgcttat
    12241 cgacgcggag tgcgggattg caggccgaac tcaggggaaa gtgcttcacg gcgagacaca
    12301 gccgcgggcc gaactcctcg ggcagcggac ggaggctgcg cgccagatcg gcacaagccg
    12361 ggcactgaaa gctgatgaac acgaccagtt gcaccggcgc gtccggtgga cccaagcggg
    12421 gatcggtggc gtcgattacg aggtcttgcc tggcagccga ctgatattcg cggagtgcct
    12481 gctccggcgt cacgccggcc gatgcggccg atcgcctgag agccgtttcg acaaacaccc
    12541 actgataggc gacgacgccg accagtgcgg ccgcgaaaaa gccaacaacc ttccacggcg
    12601 cggagctttc tgccgctgcg gtacggccga gcaggtagcg acagccgccg ccggccagct
    12661 cgcgcagctc acgcaccgtc agcccgctgg cttttttcag cgccggcacc agcgcgaggt
    12721 tggcgagctg tacgatcagg cacagcgggc aatgcggcgc ccagccgacg agcaccagcc
    12781 ccgccaagag cagacttatg cccgcaccga gagcggccag cagcagcgcg gcgagcaggg
    12841 cctcggcccg aaatgccgcg cccaaccacc acgagagcgc gagcaaggct gccagggtgc
    12901 agtaatagac gatgccccag ccggccagcg ggatgccgag ctgccacgag ctggccgact
    12961 gcaaggtcgc atcgcagccc cggccgaaca ccgtcgaaca gacgtcgagc cggcccatcg
    13021 ccccgccggc catcaagtcc caggagcgta ccagcaagta gccggacgcg gcactgccca
    13081 gtccgatcag gaccaggccg accaggctcc agcgcgttgt gcccgctgcc atcgattcgc
    13141 cccgtcagcg aaaccatgca tcaatatacg cgtcgggccc ttcgctagca ggcggcggtt
    13201 cgcattgacc gcgccgcggg tgcggcagta cgctcccgcc ccggcgcagc gctgcgcgcc
    13261 ggcgaaacaa gcagcgggat gacacggatg tcgtggcgct ggatttgtct gggttgttgg
    13321 ctgatggctg gtggttcggg ctgcgcgcgg tgggtgacga cgaaggtgga gccgcccagc
    13381 cagttgccgc aggtgaaggc ggcggcggat gcggtcatgc tcgacgtggc gttcgtcaaa
    13441 cagccggcgg ctgacgtcga ttcctacgac gcgatctggg ccgcggcgga cgagcaggtc
    13501 ctctcgaccg acctccgccg caggctggcg gccaacgggc ttcgcgccgg cgttttcggc
    13561 caggacttgc ccgcaccact gcgggagctg ctcgacaatc ccactgccga atcgcagggg
    13621 caagaggccg acgcggaagg gggcgaaggc gccggcagcc ggcggcactt gcaagttcgg
    13681 acgggagtct ggaagaaggt ctacgcctcc cagactcagc cgctcctggc cgtgctcttg
    13741 cacaccggcg gcagcgtgca cggccatcag ctgacgagcg ctcagtgcct gttcggcctc
    13801 aagacctatc ctaaagggga cggccgcgta aagctcgatc tcactccgga gatcgagcac
    13861 ggcgagctga agcccaaatt catccgcagc gaagcacagc tcgttcagag catcggccgc
    13921 gatcggctga tgctcgacga gctgcggatc gaagcgctgc tctcgccggg ccagtggctg
    13981 ctgctctcca cgacccgcga cgtcaaaggc ttgggcgaga atttctttgt cgatacgcca
    14041 agcggcgcgg tgcggcgaac gctgctctta gtccgcgtcg ctcaaacgca gcttgacgac
    14101 ctgtttgctc ccgagcaaac atccgcccct ctggcgacgc ctggggattg atcgccgccc
    14161 ttgcgctagt gtagtcctca cgctccgcgc gaggaccgcc cgctcgcgga gcgagcggac
    14221 tacacgccca ttgcgacgtt ccagctgcag gcggataatc ttacggttgt cctcattccg
    14281 cgttctgcat tccgcattgg ttggagtccg ccatgccgct cgtcccgctt cgcaaagtcc
    14341 tcgaccatgc tgcggccaac aactacggcg tggcggcgtt caacgtcaac aacatggagc
    14401 agatccaggc gatcatggag gcggccaagg agaccgattc gccggtcatc atccaggcct
    14461 cccgcggggc gcgggcctat tcgcaggaca attatctgcg gcacctgatg ctggccgcgt
    14521 ccgagctcta tccgcagatt cccgtcgtga tgcaccagga ccacggcaac agcccggcga
    14581 cctgcctgtc ggcgatcgcc aacggcttca ccagcgtgat gatggacggc tcgctgaagg
    14641 aagacggcaa gactccggcc gactacgact acaacgtgaa ggtcacgagc gaagttgtga
    14701 aacaggccca cgccaagaat gtctcggtgg aaggcgagct gggctgcctc ggctcgctcg
    14761 agcacggcga aggcgagcag gaagacggcc acggcgccgt cggcaagctc agccgcgagc
    14821 aattgctgac ggacccggac caggccgagg acttcgtcaa aaagacgggc gtcgatgccc
    14881 tggcggtggc tatcggcacc agccacgggg cctacaagtt cacccgcaag cccacgggcg
    14941 aagtgctggc gatggaccgg atcgtcgcga ttcacaagag gttgcccaac tgccacctgg
    15001 tcatgcacgg cagctcgagc gtgccgcagg acttgcagga cgagttgcgg aaatacggcg
    15061 gcgagatgaa gcagacctgg ggtgtgcccg ttgaggaaat ccagaagggc atccaacacg
    15121 gcgtgcggaa gatcaacgtc gataccgact gccgcatcgc catcacggct gcgatccgca
    15181 aggtgctcga ggagacgccc gagaaattcg acccgcgcga ctacctgaag ccggcccgcg
    15241 aggccatgaa gaaggtctgc atcgcccgga tgacctcctt cggccaggcc ggcaacgcca
    15301 gcaaaatgcg ctccgcgggg ctgatctaac tggtcgcgac acgctgatcc ctctccccgg
    15361 ggagaggcgc ctttgccctt agcttgctcg tgcctccggc cgattggacg tttcccaggg
    15421 taaggataat tcccggtgct ccaggttttt gcaaggcacc tgaagccgcg aagccgatcg
    15481 taactgcttg tctgcgctct gcttaagtcg ccgcctgacg gccggcgagc catttgagtt
    15541 tgaagggtta gagggctgcc gcgggaatta tccttacccc cgccgcccct cgccgcgtag
    15601 cataagcgtc ctcgcttgtg catcaccgaa tcagccgaag cgtggcactt tttgtgacgc
    15661 tcgcggatta ttgaccacga aaaacgcctc gtgttcaaaa attcgcccgc gattcagctg
    15721 cccgcgaagc ggcaagtctt tgccgccggc gatgttgcgc gagtttgtct ggctgtaaga
    15781 gctgcgaatt gttgaccaaa aaccgcgaac acgggggttt ctgttcaaaa aaccccgagc
    15841 cccgcctgcc gaaactcgct gggtgccacg gctgattggg tgtagccgcg ccactccgca
    15901 atccaagccg ctggccttgc tacgaattct caaagaacga ttccgccgcc gggacttcgg
    15961 ccccggtttg gaattgtaat gatccgtcca ctatctgtca agtcaaccac aacagattgt
    16021 ggtcgccttc cgcc
//
LOCUS       DASRJB010000746         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2183578, whole genome shotgun sequence.
ACCESSION   DASRJB010000746 DASRJB010000000
VERSION     DASRJB010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183578"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFB80_19695"
     CDS             <1..2083
                     /locus_tag="VFB80_19695"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN36068.1"
                     /translation="RAEMPAASAAGWEQNAIDAFLLAAMKAKEVTPAPPAGRRVLIRR
                     AYLDVLGLPPSPDQVEAFLHDLAPDAWERLVDQLLASPHYGERWARHWLDLVRYADSG
                     GFEFDVDRPEAWRYRDYVVRAFNGDKPYDRFVREQVAGDEIAQPAAGNPAPQSDEALI
                     ATGFLRLGPESGGGPRGQQESLDDLIATTCLTFLGLTANCARCHDHKFDPIPQVDYYR
                     LQAVFAPLRSISHPLVGPEAREANRAESARLEAALRPLRREKSRLEAPYLERLIADAI
                     RQMPEYLQTAWNTPADQRTEGQRLNVAQIKRTLESDTLRNKFPEQSLVALMIEDEKQR
                     HAELKSEIAALEKEKPKPSPAAMAVGESSAAPRPTYFLHRGSADARGPAVTPGVLSVL
                     SERDYAFPAPPAGARSSWRRTGLADWIASPENPLTARVMANRLWQHHFGEGLVRTPSN
                     FGDLGERPSHPELLDWLALEFIRAGWSTKALHRLMLTSQAYQMASDDSVAGLAADPEN
                     RLLWRMPRQRLEAEAIRDQVLAVAGTLDHAVGGPCVFPYIDPVLFQSSTKRTWPGRPV
                     DDASTLRRSIYVYSKRSIRYPLFEAFDQPNLVNSHDRRNRSTIAPQALLLMNNSLLTA
                     QAGQFADRLRREAGTGAADQVERAYLLALGRPPDDFERARSLEFVQTSPDALAEFCLA
                     LFNLNEFVYRP"
     gene            2080..>3166
                     /locus_tag="VFB80_19700"
     CDS             2080..>3166
                     /locus_tag="VFB80_19700"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36069.1"
                     /translation="MNDLCCTRRELLHRLGGGLAGLAFADLLARQGLLAAPPAAKAPL
                     APKPQHFPAKAKAVISIFCYGGVSQVDTFDPKPLLLARQGETMTGVGEVRATMGTPGG
                     LMPSPWRFRKHGECGMDVSELFPQVAAHADDLALIRSMHALSPAHGPALFQMNTGSIL
                     AGHPSVGSWVTYGLGTENENLPGFIVFTDYRGGPINGAPNWASGFLPAAYQGTPFRDS
                     ADPVVDLKPPVSRTEDAQRRWLELLHDLNERHAAKNPADTELAARIHSYELAFRMQSH
                     APEALDISAESAATRRLYGVDQQPTAYFGKQALMARRLVERGVRFVQIFSGGGNFEPT
                     WDAHWDLKANHSLHCAETDQPIAALIQD"
BASE COUNT          529 a         1095 c         1063 g          479 t
ORIGIN      
        1 gcgggcggaa atgccggcgg cgagcgcagc gggctgggag cagaatgcga tcgatgcgtt
       61 tctgctggcg gcaatgaaag ccaaggaggt gacgcccgcg ccgccggccg gccgccgggt
      121 gctgattcgc cgcgcttacc tcgacgtgct gggcctcccg ccgagtccgg accaggtcga
      181 ggcgttcctc cacgaccttg cgcccgacgc ctgggagcgg ctggtcgatc agctgctcgc
      241 ttcgccgcat tacggagagc ggtgggcgcg gcactggctc gacctggtgc ggtatgccga
      301 ttcgggcggt ttcgaattcg acgtggaccg cccggaggcc tggcggtatc gcgattacgt
      361 cgtgcgggcg tttaacggcg acaaacccta cgaccgcttc gtgcgcgagc aggtcgccgg
      421 agatgagatc gcgcagcccg ccgccggtaa tccagcgcca cagtcggacg aggccctgat
      481 tgcaaccggc tttttgcggc tgggaccaga gtccggcggc ggcccgcgcg gccagcagga
      541 gtcgctcgac gacctgattg ccaccacgtg cctcacgttt ctgggactga cggcgaattg
      601 cgcgcggtgc cacgatcaca agttcgatcc gattccgcag gtggactatt accgcctgca
      661 ggccgtgttc gcgccgctgc ggagcatcag ccacccgctg gtcggcccgg aagcccgcga
      721 ggcgaaccgc gccgaatcgg cgcgcctcga agcggccctg cggccgctgc gccgcgaaaa
      781 atcccgcctc gaagcgccct acctcgagcg gctgatcgcc gacgcgatcc ggcaaatgcc
      841 cgaatacctc caaacggcct ggaacacgcc ggccgaccag cggacggaag ggcagcgact
      901 gaacgtggcg cagatcaagc ggacgctgga gagcgacacg ctgcggaaca agtttccaga
      961 gcagagcctc gtcgcactca tgatcgaaga cgagaagcag cgacacgctg agttgaagag
     1021 tgaaatcgca gccctggaga aggaaaaacc caaaccctct cccgcggcaa tggccgtggg
     1081 cgaaagcagc gccgcgccgc ggccgaccta ttttctgcac cgcggcagcg cggatgcccg
     1141 cggcccggcg gtgacgcccg gcgtcctgtc ggtgttgagc gagcgcgact acgcgtttcc
     1201 cgcgccgccc gccggcgcac ggtcgagctg gcggcggacc ggcctggccg actggatcgc
     1261 ctcgccagag aacccgctga cggcgcgcgt gatggccaac cgcctctggc agcaccattt
     1321 tggcgaaggc ctcgttcgca cgcccagcaa tttcggcgac ctgggcgagc ggcccagcca
     1381 cccggagctg ctcgactggc tggcgctgga attcatacgc gccggctgga gcaccaaggc
     1441 cttgcaccgg ctgatgctga cgtcgcaggc gtatcagatg gccagcgacg acagcgtggc
     1501 cggcttggcc gctgatccgg agaatcgcct gctgtggcgg atgccgcgcc agcggctgga
     1561 ggccgaggcg atccgcgacc aggtcctggc ggtggccggc accctggacc acgccgtggg
     1621 cggaccgtgc gtctttccct acatcgatcc cgtactgttt cagtcaagca ccaagcgcac
     1681 ctggccgggc cggccggtgg acgacgcctc gaccttgcgg cgaagcatct atgtctactc
     1741 gaagcggagc attcgctatc cactgttcga ggccttcgat cagccgaacc tggtgaattc
     1801 gcacgaccgc cgcaatcgct cgacgatcgc cccgcaggcc ttactgctca tgaacaacag
     1861 cctgctcacc gcccaggccg ggcagtttgc cgatcgtctc cgccgcgaag cgggcactgg
     1921 cgccgccgac caggtggagc gggcctatct gctggccctg ggccgaccgc cggacgactt
     1981 cgagcgtgcg cggtccctgg agttcgtgca gacgagtcct gacgcgctgg ccgaattctg
     2041 tctggccctg ttcaacctga acgagtttgt ctaccgccca tgaacgacct gtgctgcacg
     2101 cgaagagagt tgctccaccg gctgggaggc ggcctggcgg gactggcctt cgccgacttg
     2161 ctcgcgcggc agggcctgct cgccgcaccg ccggcggcca aagcgccgct cgctccaaag
     2221 ccgcagcact ttccggccaa ggccaaggcg gtgatctcga tcttctgcta cggcggcgtg
     2281 agccaggtgg atacattcga ccccaagccg ctcttgctcg cgcggcaggg cgaaacgatg
     2341 accggcgtcg gcgaagtgcg ggccacgatg gggacgcctg gcggcctgat gccttcgccc
     2401 tggagattcc gcaagcacgg cgagtgcgga atggacgtct ccgagctgtt tccgcaggtg
     2461 gcggcgcacg ccgacgacct cgcgctgatc cgctcgatgc acgccttgag tcccgcgcac
     2521 gggccggcgc tgtttcagat gaacacgggc agcattctcg cggggcatcc cagcgtgggc
     2581 agctgggtga cgtatggcct gggcaccgag aacgagaacc tgccggggtt catcgtcttc
     2641 accgattatc gcggcgggcc gatcaacggc gcgcccaact gggccagcgg cttcttgccc
     2701 gccgcgtacc agggaacgcc gttccgcgac agcgccgacc cggtcgtcga tctcaagccg
     2761 ccggtgtcgc gcacagagga tgcccagcgg aggtggctgg agctgctgca cgacctcaac
     2821 gagcgccacg cggccaaaaa tcccgccgat accgagctgg cggcgcggat tcactcgtac
     2881 gagctggcct tccgcatgca gagccacgcg cccgaggccc tggacatcag cgcggaatcc
     2941 gccgccaccc gccgccttta cggcgtggat cagcagccga cggcctattt cggcaaacag
     3001 gccttgatgg cccgccggct ggtcgagcgg ggcgtgcggt tcgtgcagat tttctcgggc
     3061 ggcggcaact tcgagccgac ctgggacgcc cactgggacc tcaaggccaa ccacagcctg
     3121 cactgcgccg aaaccgacca gcccatcgcg gccctcattc aggacc
//
LOCUS       DASRJB010000747        10018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1066753, whole genome shotgun sequence.
ACCESSION   DASRJB010000747 DASRJB010000000
VERSION     DASRJB010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066753"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..744
                     /locus_tag="VFB80_19705"
     CDS             157..744
                     /locus_tag="VFB80_19705"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HZN36070.1"
                     /translation="MHPILSRNLYFVKEHVGFFKAANNYDVLDPETNQLILNCREERL
                     GFFTKLLRFSDYKRLTPFEVIIRTPEGAPVICVKRGVALFLSKVDVLDENGQRIGGFA
                     QKFFSLFDKFTVVGANDQPVCVLKGNWTSWDFRFMAGEQELAKVSKKWAGLGKELFTS
                     ADNYVLSISPSVPPDNPIRGLILGAVMCIDLVLKE"
     gene            complement(796..1038)
                     /locus_tag="VFB80_19710"
     CDS             complement(796..1038)
                     /locus_tag="VFB80_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36071.1"
                     /translation="MTIEQVRKLHQARPFQPFRMHLADSRSLDVVHPELLAISEPGRT
                     VIVVTGDESLYEVVDLLLVTSLEVLNGRAKAGRRKH"
     gene            complement(1060..2622)
                     /locus_tag="VFB80_19715"
     CDS             complement(1060..2622)
                     /locus_tag="VFB80_19715"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZN36072.1"
                     /translation="MSADPAGQPPAPSPAPESSAPPAGAGPLARFKRPAAQPDAPQPA
                     EAALPVAPAAETPPAGPSGNRPPRGGQRPKSQRQKHREEDAQVDRQIKAEREPREEFA
                     RRQHVPVPNLRQMSEELEAEVAAALGGLSLDKIVEGDLKTASARLENESRHRAQVVDI
                     HGDDVFFALGGKNQGVASVRSFATPPTPGDMLDVIVTGLNAEDNLYLLSIPGGTLVAG
                     GDWNDIAEGSLVEARVSGANTGGLECQVNKLRAFIPASQVALYRTENLADYIGQKLVC
                     VVTQSDQARGNLVLSRRAVLEREKEESKKQLLAELGPGQMREGTIRKLHDFGAFVDLG
                     GVDGLIHVSQLSWERVKHPSEVVQEGQKVRVRIEKIDPDTGKISLSLKNPEEHPWTGI
                     EQRFPVGSTVKGPVSRVAQFGAFVKLAPGVEGLIHISELAHHKVYKVENVVKEGDEVE
                     CKVLTVDAEAQRIGLSLKATLAKPEKAEGKSKDQPEVDEPPREPAVPKRSGPLKGGTG
                     KPSGGEQFGLRW"
     gene            2830..4275
                     /locus_tag="VFB80_19720"
     CDS             2830..4275
                     /locus_tag="VFB80_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36073.1"
                     /translation="MSRILLSLVVFSHLSQAAAAQFNELAAKIPGTANAIVLLDGPKL
                     LSSPLATKEGWKEKYEQAFASGLVSIAPDTQQLILASQIDYEFMRPVWEAAVGDLGRE
                     RSLADVARRTKGTIDPLGDTPAVALRDDAFLVAFGPKRLGVMSPANRQSVARWLREAS
                     SRTQSVLSPYLQGALTASQTSGIVMAFDLQDAVPPDVIRAKLATSPALAGKNINLDAA
                     AKALASIRGLSLEVAVTDGAFGRLMIHFNDDASILAPVAKPLLLEILASLGATIDDIE
                     QWQVESEPQRITLRGPLSTAGRKRVFSLIDQPTSALIAIDKSGTGADLQDQAQKGMAS
                     LAYYRSINSILDEVREKSKNAKTFGENALWFDKWARKIDRLPLLNVDAELLAFGRSVG
                     VSLRNMATSLRGIGINTAARSAQIYDSGSYGYNGYSYYAEWRDVDDERRAVKADEKAK
                     GAISARSTAGDLATENARIRQVMSQKYQLEF"
     gene            4411..5895
                     /locus_tag="VFB80_19725"
     CDS             4411..5895
                     /locus_tag="VFB80_19725"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN36074.1"
                     /translation="MHLRAWLMSVAFGWGTLLATACAQSPGEVGGLPPPPNDGGEFFI
                     PSPLDASYTQPPLAPPLDPPGGSPAKPPVLDPPPLKYPLSAVVSGVFQADGVVFDQSE
                     RNRLRAADGGVGPIENGADFRRARIGVRSAVYNNMNAFMQMDFAFPGRPTFTDVWVEW
                     TDLPVLGTVRVGQWKQPFSLEVVSSFRYTTFMERSSLFQAFTPFRHIGVGFYNHAEDL
                     MSTWAASYLRTGQDQFGDSLSTRGGNGFAGRLTSLLWYDEPEGRSYLHVGGAYFLNTP
                     PLHAIAFRSIPEIFVGQNRSNAADVGTAGFAVPFLFDGTPFFVNTGLILDVEHVHTFG
                     LEWLWVHGPLSVQAEWMSAAVDQAVAQTATLDGAYLQVGWFLTGEHRPYDRVNGAIDR
                     VKPFEDFFLVRTDHGLAHGMGAWEVAARISHIDLDSGNISGGKMDNLTFGINWYCNPY
                     CKVVFNYIHSWRESPTTPPHLPPGPPVAVASHCNAFGIRAQVDF"
     gene            6036..7598
                     /locus_tag="VFB80_19730"
     CDS             6036..7598
                     /locus_tag="VFB80_19730"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN36075.1"
                     /translation="MSKLGSKNGLLLLVTLAAAGVLLVWLPPMLAEQYDRIKSLGPPW
                     TYFYFALIGTGAAILLTLAVMVVWKLASATWMKEARQSRGAKDPSQLSAGEQRQEVAD
                     NLAAVADLQAGAALPADVRQQLESLVARVEEKQASQKLEIVAFGTISSGKSSLLNALA
                     GRDVFQTDPRGGTTQQRLEIPWPNDDRAVLVDTPGLGEVEGAERVAVAAQSAEGADLV
                     LLVVDGPLRQSEHELLAKLGAMEKRVLVCLSKADWYDDDERQKLLGQISGQVHDYVAA
                     EDVLAVRSQPTVRTRVRVLAGGDEHQEQVPVPPDIGPLARRMLQVVRTSGNELVLANL
                     LLRSRGLVEEARRQVQESLDRRAWELVDRYTWACGAVAALSPLPVLDLFASSALTAKM
                     VVDLARVYRQEMDLNIAVNLLAQLGKNLIAILGVNAAAPAVAAAVASLLKTIPLAGSI
                     AGGALQGLVQALVTRWIGAVFIGYFQAEMKLPPEGLANLARQEWQRLTNPAELLKFLQ
                     EARSRLRGEPRE"
     gene            complement(7667..8476)
                     /locus_tag="VFB80_19735"
     CDS             complement(7667..8476)
                     /locus_tag="VFB80_19735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HZN36076.1"
                     /translation="MSLNSVMQTALSGMQAATASVGVTAHNLANLQTAGFERSRVQLA
                     TLPLGGGVMVSAIVLDPAQGPIASGDQLPLLALEGEGLFILEGDGGERLFTQGGRFSL
                     NAAGELVTAGGDRVLGYGLDTDGQIDRSELSPLTIRLGSQVASAGGRAATLQSYSITR
                     GGRIVGRYSDGVNRTLGQLRLARFSNPAGLTQRAGNRFQATAASGEPRESDPGEAGAG
                     EVVAAATELSNVDLGRQLIELTLANQLFRANAAVFRTADEMLGEMFFPWRR"
     gene            complement(8550..9869)
                     /locus_tag="VFB80_19740"
     CDS             complement(8550..9869)
                     /locus_tag="VFB80_19740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36077.1"
                     /translation="MRAVSLTFVLLCCTTAAAGDWPQFRGPRGDGTADAKNLPTTWGG
                     FGTIAWQAEIPGRGWSSPIVLGERIWLTHAETTALTAQQREKRLSDSVLKDFAEQFQA
                     HAAVTLYAAEFDVKSGELLRNIELVTCEQPPPIHATNTYASPTPATDGERLYCHFGPL
                     GTAALDLKSGHVVWKERFVFDDITGPGSSPLLVRNKLIFPCDGVDQQFVVALDKRTGQ
                     VAWKTPRPKIDAAEGKHRRAFSTPLLVEHAGREQVVVPAAQWVCSYDPGSGQQLWRVN
                     FGDGHATIPRPVFRDGIVYVCTGYLKPQLWAIRVDGSGDVTESHVVWKYDKQVPEISS
                     PVIAGGEIYFVSALGVATCLDLHTGELVWQHRLGGNYGASPLAADGKLYFTSQEGVTT
                     VLKPGREYHELAKNQLFGQTLASLAVCGESLLIRADRTLFCVRSAAP"
BASE COUNT         1633 a         3334 c         3289 g         1762 t
ORIGIN      
        1 gactgggtcg gcacgggctg acccacgacc gtccggatcg agaactacga gatccggcgg
       61 taacagagtc cgcgcggcca gttgcctgcg gcgcggtgaa cgacgataat cccgccgttt
      121 cccgctccct cccttggcaa ctcgcgcgag actgctatgc atccgatcct gagccgcaac
      181 ttgtatttcg tgaaagagca cgtcggcttc ttcaaggccg cgaacaacta cgacgtgctc
      241 gaccccgaga cgaatcagct catcctcaac tgccgcgagg agcggctggg gttcttcacg
      301 aagctgctgc ggttctcgga ctacaagcgg ctgacgccgt tcgaagtgat tatccgtacg
      361 ccggaagggg cgccggtcat ttgcgtcaag cgcggcgtcg cgctgttcct gtcgaaggtc
      421 gatgtgctgg acgagaacgg ccagcggatc ggcggtttcg cccagaagtt cttctcgctg
      481 ttcgacaagt tcacggtggt cggggcgaac gatcagccgg tgtgcgtgct caagggcaac
      541 tggacgagct gggactttcg cttcatggcc ggggagcagg agctggccaa ggtaagcaag
      601 aagtgggccg gcttagggaa agagctgttc accagcgccg acaactacgt cctgtcgatc
      661 tcgcccagcg tgccgcccga caatccgatc cgcgggctga tcctcggggc ggtgatgtgc
      721 atcgacctgg tgctgaaaga atagctggct gtcagcggtt aacttgcgac aatcggttta
      781 cgctgaagtg cggcttcaat gcttgcgcct tcccgccttg gctcgaccgt tgagcacttc
      841 caagctggtc accaaaagca ggtccacaac ttcgtacagg ctctcgtcgc cggtaaccac
      901 tatgaccgtc cgaccgggtt cgctaatggc cagcaattcg ggatgaacaa catccagtga
      961 gcgactgtcg gccaagtgca tgcgaaaagg ttggaacggg cgtgcctggt gcaacttacg
     1021 aacttgctcg atcgtcatcg atgaccctcc tggttgattc taccatcgca ggccgaactg
     1081 ctcgccgccg ctcggtttgc cggttccgcc tttgagcggg ccgctccgct tgggcacggc
     1141 cggctcgcgc ggcggctcat cgacctcggg ctggtccttg gacttcccct cggccttttc
     1201 gggcttggcg agcgtggcct tcagcgagag gccgatccgc tgggcctcgg catcgaccgt
     1261 gagcaccttg cactcgactt cgtccccttc cttcacgaca ttttcgactt tgtagacctt
     1321 gtggtgggcc agctccgaaa tgtgaatcag cccctcgacg cctggcgcga gcttgacgaa
     1381 cgcgccgaac tgtgcgaccc gcgagacggg gcctttgacc gtgctgccga ccgggaaccg
     1441 ctgctcgatg ccggtccagg gatgctcctc ggggttcttc agcgagaggc tgatcttgcc
     1501 ggtgtccggg tcgatcttct cgatccgcac gcggaccttc tgcccttcct gcacgacctc
     1561 gctgggatgc ttgacgcgct cccaactgag ctggctgacg tggatcaagc catcgacgcc
     1621 gcccagatcg acgaaggccc cgaagtcgtg aagcttgcgg atcgtgccct cgcgcatctg
     1681 gccgggtccg agctctgcca gcagctgctt cttcgattct tctttctccc gctccagcac
     1741 ggcccgccgg ctgagcacca ggttgccgcg ggcctggtcg gattgggtca cgacgcagac
     1801 cagcttctga ccgatgtagt cggccaggtt ctcggtgcga tagagggcta cctggctggc
     1861 gggaatgaac gcgcggagtt tgttgacctg gcattccagg ccgccggtgt tcgcgccgct
     1921 cacgcgggcc tcgaccagcg agccctcggc gatgtcgttc cagtcgccgc cggcgacgag
     1981 cgtcccgccg ggaatcgaaa gcagatacaa gttgtcctcg gcgttcaggc cggtgacgat
     2041 cacgtcgagc atgtcgccgg gcgttggcgg cgtcgcgaaa ctgcggacgc tggccacgcc
     2101 ctggttcttg ccgccgaggg cgaagaacac gtcgtcgccg tgaatatcca cgacttgcgc
     2161 ccggtgccgc gattcgttct ccagccgtgc gctggccgtc ttcaggtcgc cctcgacgat
     2221 cttgtcgagc gaaagtccgc ccagggctgc cgcgacttcg gcttccagtt cctccgacat
     2281 ctgccgcagg ttcggaaccg gcacgtgctg ccggcgggcg aactcttccc gcggctcccg
     2341 ctcggccttg atctggcgat cgacctgcgc gtcttcttcg cggtgctttt gccgctgcga
     2401 tttcggccgc tgcccgccgc gcggtggacg gttgccgctc ggacccgccg ggggcgtctc
     2461 tgccgctggt gcaaccggca atgccgcctc cgccggctgc ggcgcatccg gctgagcagc
     2521 gggccgcttg aagcgcgcca gcgggccagc gcccgcggga ggagcggaag actcgggagc
     2581 gggcgagggg gcaggcggct gcccagcagg atcggcggac atcgtcgaaa ctatgcaggt
     2641 ggcaggagcc gttctggtgg cggctcgcgt gagttcttgg cgatgctcat gcccttgcgc
     2701 tgtgcatatc gcctacaatc ccagacagtc tagccgccgt tcggcgagca cggtaggagc
     2761 ggtcccgcgc ggggatcgtg gcaggatgcg gcgtttacct tcgtgaagca cacctattag
     2821 ggcaacccca tgagccgtat cctgctgagc ctcgtcgtgt tcagtcactt gtcgcaagcg
     2881 gccgcggccc agttcaatga gctggcagcc aaaattcccg gcacggcgaa cgcgatcgtc
     2941 ctgctggacg ggccgaagct gctttcgagt ccgctagcga cgaaggaggg ctggaaggag
     3001 aagtacgagc aggcgtttgc ctcggggctg gtctcgatcg cgccggatac acagcagctg
     3061 atcctggcgt cgcagatcga ctacgagttc atgcggccgg tctgggaggc ggcggtcggc
     3121 gacctgggtc gcgagcgctc gctggccgac gtggctcgca ggaccaaggg caccatcgat
     3181 ccgctcggcg acacgccagc cgtggccctc cgcgacgacg cgtttctcgt ggccttcggc
     3241 cccaaacgct tgggcgtgat gtcgcccgcc aatcgccagt cggtcgctcg ctggctgcgc
     3301 gaagccagca gccgcacgca gtcggtgctg tcgccctatt tgcagggcgc gctgactgcg
     3361 tcgcagacgt cggggatcgt gatggcgttc gacttgcagg acgcggtgcc gccggacgtg
     3421 attcgggcca agctggcgac aagccccgcg ttggccggca agaatatcaa tctcgatgcc
     3481 gcggccaagg ccctggcgag catccgcggg ctgtcgctcg aagtggcggt gaccgacggt
     3541 gcgttcggcc ggctgatgat ccacttcaat gacgatgcgt cgattctggc gcccgtggcc
     3601 aagccgctac tgttggaaat tctcgccagc ctgggtgcga cgatcgacga cattgagcag
     3661 tggcaggtgg agtccgagcc gcagcggatt acgctccgcg gcccgctcag cacggcgggc
     3721 cgtaagcggg tattctcgct gatcgatcag ccgacctcgg cgctgatcgc catcgacaag
     3781 agcggcacgg gagccgactt gcaggaccag gcccagaaag gcatggcctc gctggcctat
     3841 taccgttcga tcaactccat cctggacgag gttcgcgaga agagcaagaa cgccaaaacg
     3901 ttcggcgaaa acgccctgtg gtttgataag tgggctcgca agatcgaccg gctcccactg
     3961 ctcaacgtcg atgccgaatt gctcgccttc ggccgcagcg tgggcgtctc cttgcggaat
     4021 atggcgactt ccctgcgcgg aatcggaatc aacacggccg ctcgcagcgc gcagatctac
     4081 gacagcggaa gctacgggta caacggctac tcgtactacg ccgaatggcg cgacgtcgat
     4141 gacgagcggc gggccgtgaa ggccgacgaa aaggccaaag gagcgatctc cgcccgctct
     4201 accgccggcg acctggccac cgagaacgct cgcatccggc aggtcatgtc ccagaaatac
     4261 cagcttgagt tttgaccagg cggcgagatt acaaatccgc cggttgcgat ggatcggctt
     4321 tgacagcctc gcaacctctc gcgtacaagc cgcccactcg cctgcgggag ggcgggagcc
     4381 ctctaccggc ggatcgccga aggtcgagcc atgcacctgc gcgcctggct gatgtcggtt
     4441 gcgtttggat ggggaacgtt gctagctacc gcctgcgcac aatcccctgg tgaagtcggc
     4501 ggcctgccgc cgccgcccaa cgatggcggc gagttcttca ttccttcgcc gctggatgcc
     4561 agctacacgc agccgccgct agcaccgccg ctcgatccac cgggaggcag tcccgccaag
     4621 ccgccggtgc tggatccacc gccgctcaag tatccgctgt cggccgtcgt ctccggcgtg
     4681 tttcaagcgg acggcgtggt gttcgaccag agcgagcgga accggctgcg ggccgccgac
     4741 ggcggcgtcg ggccgatcga gaacggggcc gactttcgcc gggcgcggat cggcgtccgc
     4801 agcgccgttt acaacaacat gaacgcgttc atgcagatgg acttcgcatt tcccggccgg
     4861 ccgacgttca ccgacgtttg ggtcgagtgg acggacctgc cggttttggg caccgtccgc
     4921 gtcgggcaat ggaagcagcc gttcagcctg gaggtcgtga gcagcttccg ctacacgacg
     4981 ttcatggagc ggtcgagcct gttccaggcg ttcacgccgt ttcggcacat cggcgtgggt
     5041 ttctacaacc acgccgagga cctgatgagc acgtgggccg cttcctacct ccgcacgggg
     5101 caggaccagt tcggcgactc gctgagcacg cgcggcggca acgggttcgc cggccgcctg
     5161 acgagcttgc tctggtacga cgagccggag ggccgcagct acctccacgt cggcggagcg
     5221 tattttctga atacgcctcc cctgcacgcg attgcctttc gctcgatccc ggaaatcttc
     5281 gtcggacaga atcgcagcaa cgccgccgac gtaggcaccg ctggcttcgc cgtgccgttc
     5341 ctgttcgacg gcactccgtt cttcgtcaat accggcctga tcctagacgt cgagcacgtg
     5401 cacacgttcg gcctggaatg gctgtgggtc cacgggccgc tgtcggtgca ggccgagtgg
     5461 atgtcggccg cggtcgatca ggcggtggcg cagaccgcga cgctcgacgg cgcttacctg
     5521 caagtcggct ggttcctgac cggcgagcac cgaccctacg accgcgtgaa tggtgcgatc
     5581 gaccgcgtga agcccttcga ggacttcttc ctggtccgca cggaccacgg cctcgcccac
     5641 ggcatgggcg cttgggaagt ggccgcgcgg atttcgcaca tcgacctgga cagcggcaac
     5701 atctcgggcg gcaagatgga caacctcacg ttcggcatca actggtattg caatccgtac
     5761 tgcaaggtcg tcttcaacta cattcattcc tggcgcgagt cgcccacgac accaccgcac
     5821 ctcccgcccg gcccgcccgt cgccgtcgcc agccattgca atgccttcgg catccgggcg
     5881 caggtggatt tctgaggcaa tgggagcctc gcctgttcgc tctcgaattt ggcgcagaca
     5941 cgaatacctg cggcatggtt aagatcgtgc gtggcccagc gaatggtctt gggcgtcgcc
     6001 caggattcga gccggtcaac cctcggtcag tctccatgtc caaactcggc tccaagaacg
     6061 gcctgttgct gctcgtcacg ctcgcggcgg cgggcgtgct gctggtctgg ctgccgccga
     6121 tgctggcgga acaatacgac cgcatcaaat cgcttgggcc gccctggacg tatttctatt
     6181 tcgccctgat tggaaccggg gcggccatcc tgctgacgct ggcggtgatg gtcgtctgga
     6241 agctcgccag tgcaacctgg atgaaggagg cccggcagtc gcgcggggcg aaggacccca
     6301 gccagctctc ggccggcgag cagcggcagg aagtcgccga caacctggct gccgtcgccg
     6361 acttgcaagc cggcgcggcg ctgccggcgg acgtccgcca gcagctggag tcgctcgtcg
     6421 cccgcgtgga ggaaaagcag gctagccaga agctggagat cgtcgccttc ggcaccatct
     6481 ccagcggcaa gtcgtcgctg ctcaacgccc tggccggccg cgacgtcttt cagaccgatc
     6541 cccgcggcgg aacgacgcag cagcggctgg aaattccctg gccgaacgac gaccgcgcgg
     6601 tcctggtcga tacgccgggc ctgggcgagg tcgagggcgc cgagcgggtc gctgtcgcgg
     6661 cccagtccgc cgagggcgcg gacctcgtgc tcctcgtcgt cgatggcccg ctccgccaga
     6721 gcgagcacga gctgctcgca aagctcgggg cgatggagaa acgcgtcctg gtctgcctga
     6781 gtaaggccga ctggtacgac gacgacgagc ggcaaaaact cctcggccag atttccgggc
     6841 aagtgcacga ctacgtcgcg gccgaagacg tgctggccgt ccgctcgcag ccgacggtgc
     6901 ggacgcgcgt ccgcgtgctg gccggcggcg acgagcacca ggagcaagtt cccgtgccac
     6961 ccgacatcgg cccgctggcc cggcggatgc tgcaagtcgt ccgcacgagt ggcaacgagc
     7021 tggtgctggc caacctgctg ctcaggagcc gcgggctggt ggaggaggcc aggcggcagg
     7081 tgcaggagtc gctcgaccgc cgggcctggg agctggtcga tcgctacacc tgggcctgcg
     7141 gtgcggttgc cgccctcagc ccgctgcccg tgctcgacct gttcgcttcc agtgcgctga
     7201 cggcgaagat ggtcgtcgat ttggctcgcg tctatcggca agagatggac ctgaacattg
     7261 cggtgaacct gctggcgcag ctcggcaaga acctgatcgc gatcctcggc gtcaacgccg
     7321 ctgctcccgc cgtggccgcg gcggtcgcct cgctgctcaa gaccatcccg ctggccggat
     7381 cgatcgctgg cggcgcgctg cagggcctcg tgcaggcgct cgtcacccgc tggatcggag
     7441 cggtcttcat cggctatttc caggccgaga tgaaactccc gcccgagggg ctcgccaacc
     7501 tcgcccggca ggaatggcag cggctgacca accccgccga gctgctcaaa tttctccagg
     7561 aagcgagaag ccgcttgcgc ggcgaaccga gagagtagag ctagtgggta gggctagagc
     7621 tgtcgctgct taccgccccc ctggccctac actctagccc tagcccttac ctccgccacg
     7681 gaaagaacat ctcgccgagc atttcgtccg ccgtgcggaa gacggctgca ttcgcgcgga
     7741 acagctgatt ggcgagcgtc agctcgatga gctggcgtcc cagatcgacg ttggaaagct
     7801 cggtcgccgc cgcgactacc tcgcccgcgc cggcctcgcc cggatcggac tcgcgcggct
     7861 cgcccgaggc cgccgtcgcc tggaacctgt tgcccgcccg ctgggtcagg ccagcgggat
     7921 tgctgaatcg cgccaatcgc agttgcccca gcgtgcggtt cacgccgtcg ctgtaccggc
     7981 cgacgatccg gccgccgcgc gtgatggaat agctctgcag cgtcgcggcc cggccgccgg
     8041 ccgacgcgac ttgcgacccg aggcggatgg tgagcggcga aagctcgctg cggtcgatct
     8101 ggccatcggt gtccaggccg tacccgagca cgcggtcgcc gccggccgtc accagttccc
     8161 ccgccgcgtt cagcgagaac cggccgccct gggtgaagag ccgctcgccg ccgtcgcctt
     8221 ccaggataaa cagcccttcg ccttccaatg ccagcagcgg cagttgatcg ccgctggcaa
     8281 tcggcccttg agcaggatcg aggacgatcg cgctgaccat cacgcccccg cccagcggca
     8341 gcgtcgccag ttggactcgc gatcgttcga agccggctgt ctggaggttg gcgagattgt
     8401 gcgcggtcac tccgaccgat gccgtcgccg cctgcattcc ggacagagcc gtctgcatga
     8461 ccgagttcaa gctcatggcc ccccctccgc cgatttcggg acagaaaagt cccatcacaa
     8521 atctacgtca agaacgggat ggcggccggt tagggcgctg ccgaacgcac gcaaaacagc
     8581 gtccggtcgg cgcggatcag cagcgactcg ccgcagacgg ccagcgaggc cagcgtctgt
     8641 ccgaagagct gattcttggc cagctcgtga tactcgcggc cgggcttgag gacggtggtg
     8701 acgccctcct gactggtgaa atagagcttg ccgtcggcgg ccagcgggct cgcgccataa
     8761 ttgccgccca ggcggtgctg ccacaccagc tcgcccgtgt ggagatcgag gcaggtcgcc
     8821 acgcccagcg ccgagacaaa gtaaatctca cccccggcga tcaccggcga cgaaatctcc
     8881 ggcacctgct tgtcgtattt ccacacgacg tggctctccg tcacgtcgcc cgatccatcg
     8941 acgcgaatcg cccacagttg tggcttgaga tagcccgtgc agacatagac gatgccgtcc
     9001 cgaaagaccg gccgcggaat cgtcgcgtgg ccgtcgccga agttgacgcg ccacagctgc
     9061 tgcccgctgc ccgggtcata ggagcagacc cactgcgcag ccggcacgac gacttgttcg
     9121 cggcccgcgt gctcgaccag tagcggtgtg ctgaacgcgc ggcggtgttt cccttccgcg
     9181 gcgtcgatct tgggccgcgg cgtcttccag gcgacttgcc ccgtacgctt gtcgagcgcg
     9241 accacgaatt gctgatcgac gccgtcgcag gggaagatca gtttgttccg caccaagagc
     9301 ggcgagctgc ccggtccggt gatgtcgtcg aacacgaacc gctccttcca caccacgtgg
     9361 cccgacttga gatccagggc cgccgtgccg agggggccaa aatggcaata cagccgctcg
     9421 ccgtcggtcg ccggcgtggg cgaggcgtac gtgttggtgg catgaatcgg cggcggctgc
     9481 tcgcacgtga cgagctcgat gttgcgcagc agctcgccgc tcttgacgtc aaattcggcc
     9541 gcgtacagcg tgaccgcggc gtgcgcctgg aactgctcgg caaaatcctt aaggacgctg
     9601 tcggacagcc gcttctcgcg ctgctgggcc gtgagggcgg tcgtttcggc gtgcgtcagc
     9661 cagatccgct cgcccagcac gatcgggctc gaccagccgc ggccgggaat ctccgcctgc
     9721 caggcgatcg tgccgaagcc gccccaggtc gtcggcagat ttttggcgtc ggccgtgccg
     9781 tcgccacgcg ggccgcggaa ctgcggccag tcgccggccg cggctgtcgt gcagcagagg
     9841 aggacgaacg taagcgaaac ggcgcgcatc gggcttccct cggctggccg acgaatccag
     9901 cttatcacac ggaaggccct cgccctggca gccgcgatta gttgccctgg tcgctgccgt
     9961 agccgttgcc gttgtcgttg ccgaaccgct tggtcaagag gtgcggatca gtgaggtc
//
LOCUS       DASRJB010000748         7472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_783039, whole genome shotgun sequence.
ACCESSION   DASRJB010000748 DASRJB010000000
VERSION     DASRJB010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783039"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..5697)
                     /locus_tag="VFB80_19745"
     CDS             complement(154..5697)
                     /locus_tag="VFB80_19745"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36078.1"
                     /translation="MLRCPFCEADLSETGSFRLRGGRCPQCGSILNWAQDSAEESSQQ
                     PAAPEGSAPAAPPVDEDAMSMRDIIRTLVSRGGGDEDEDSPPPSLLSPAPGAAPPSRP
                     RLPTPAATKTVNTGPPAGGEGSSPEQVDRIWSKSITSVATKPSMTIKASSTEPEGAIS
                     DLYIRPFRIALPGEITQATNEFELQEVIGEGGVGVVYAARQASIDRTVAIKMLRHEFA
                     HKRDHRNKFLTEAMVTGELDHPNIVPIYDLGTSELGSLFYAMKRVRGTPWSDVVRQQS
                     LAENLRILMSVADAIAFAHSRGVVHRDLKPENVMLGDFGEVLVMDWGIALSTATFIRS
                     NKIGQSTSMGGTPAYMAPEMATGPLTAIGQHSDVYLLGALLFEIVTGRTPHTGKDVMS
                     CLYAAARNEIQPTDKSGELLDVAYRAMATKPDDRYASVQDFQAAVREYQSHSESVVLS
                     TRADEELKQARQTKDYQDFSRALFAFQEAHALWSGNERAKSGEIEARMAYAERALEKG
                     DFDLGLSLLDERQPVQAGLHGKLAAARHEREARQARIKGLRRLALGLAALVMLTLSVG
                     LYFISGQNRTISEQYKSLESTNKDLTQARGRLDEEIKTVQESEKKLLKSKSDLLATNQ
                     DLKVTRVAALNEARTARQEEQNAAAASYRAQIGVAAERIANNSFLDADQLLNDYQGAT
                     ARWSYFRNWEWGYLKHLCDRQAGQQDVKTRIESLARSADGSRYVAGTATGKVLVWEID
                     FPQCTFRPKPTLNLGGPVLAVALSASGDQLAAASDADGGIALYQRSADGSYDEASKTV
                     LPDTGGVLSLAFSPSGKLLVSSSRVGTAQVWSLETGKGVPTEGGHTGPIWSAVFTAGG
                     DTVVTGGDDGTVRLWSLTGKKKGIYRGHKGPVYAVAAAPRGNWIASAGRDRDVHVWRA
                     DMNLEINFGQIAKQLEQERLGKLVPEPRQSFHTPTFPPLTGHTGEVRALSFSADGTWL
                     ASGGNDNTVKLWKFAPNLTSIDLATTFRGHGGWVRGCVLAADPRYAVSSSLDGFVKIW
                     DAQEYEEVRALRGHEDAVLWATFSRSGQRMVTAGRDHKALVWNAGERRPLKVFDEGEE
                     LDAAGPAAPAARIKEGHEFLVSSVAFLPDDRHVLTSAGDGTVRMWDRRTGGQVRRLRN
                     TGTLSVLALSADGEWIVTGSDAAELRQPQSAQLWSANDPQSEPIKLTGHPGEVSAAAF
                     SPDRDLETLRIATGDVLGGVRLWRFDAGSRSWQATPVRGHSTGYPISAVKFTPDGRRL
                     LTASQDRIVLIHDALTGQTLPPPLKHQGGVKAIDISADSRRALSLSSPGKGSYRISLW
                     DLASGSEQFRDVNLPDEALTSAVFHPAQTSAILTSASANRNSSRFWRWDFARNEVQPL
                     WPQNQVRGAIWAASFSPDGSHLLAAGGSQARLITAQGQLEKTFSSHGAVTSAEFSPDD
                     KLLATSSLDGDVKLWYADPQHPEFGRVAVKLAHPHSNGSQWSAVNSVAFSPVIADNGY
                     RLVTAGNDGTARLWRYAGGAITPEGDVLRHTDRVHSAQFSPDGNFVLTASKDKFARVW
                     NIAAQPPQEPLHLSHDEAVLFAAFSPDGTRVITGCNDNDAYVWDLNPVRSFKLQGHTA
                     AVTSAAISPNGLRAITGSQDGMAKLWDLESSKEVKEVLSLKRHAAELTSAHFSPDGSR
                     ILTSSLDGLALVWPSIAIGPSVKLSSARREIPHLAGLHVIDDEARIFDPDAGDLAGGS
                     LSIWPNSTLTATTLDLAAGGDIEVAGGQNVVLTGPAGRRAVAQIERPAEPAGCLRLRL
                     LPGCEAADAQRLLRALAIRTGGPLAGGVTLRVQIIDAEGRASFIAETVVASAEAPSGE
                     RTASR"
     gene            complement(5734..>7472)
                     /locus_tag="VFB80_19750"
     CDS             complement(5734..>7472)
                     /locus_tag="VFB80_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36079.1"
                     /translation="PLQVAPGTGNLTLVASGDTWVNVVGGGIDATKLSVQGSQTVGLS
                     TSAGSIELDGDSFQQRTLSFLSVTALGAGSNITFGDTKIAADELHLLAAGNIGESDNP
                     IFFAAQQLRTLSAGSQYLAVDGSTTVQAIQSFGEVSLQSGDFLVAESGFIFATQTVTV
                     ETAALLGGDGKISANLVLVRGVLSPGNFEPAGTRENPIDGDASVLTIFGNVTVTDTGQ
                     MVFDVNPPYETAGTDYDQVIVNGTLTLGDPDDPSEPVGNDGPLLVLRGGATLQQSIQG
                     LQLVTGNVQGMFRIDDNPLNPLSIDKRTGGIKATVGNFTGSLLVNVPTPGLLIGELTL
                     VPPIQRVIFKPPTILIPVFTSLVRLQPPVQPPPPLTPPSQPPPLEFKTQAVRLRYLEI
                     RLVVPNGDGSFREEFVMNLSADMLTDLLAVFRRLPDDRYRVYLMLAGNAEERLVIDVL
                     VRDGKPVEPLEQAGDEAAPEMALPPVPAAPPAPVPHQHHEGTSGPMALPPLPAAADVP
                     MPLGYDQQPAEHFWTPAEQPAAGRLFAGVGLAAAAIAAGRSPGRWKGQVEQAAARIGA
                     SPAHWRWWRKSA"
BASE COUNT         1137 a         2469 c         2583 g         1283 t
ORIGIN      
        1 agaagaagta ggccgtaggg tgggtcaagc gagtcctcga gcgcggaatc acgcggcgac
       61 cgtgcttagg cgaaggtgta agagcggagc gaatcacacc cttccccggc cggtgcattt
      121 cgcttcgttc atgcaccgcc aagcccaccg tacttaccgc gaggccgtac gctcgccgct
      181 gggcgcctcg gccgaggcga cgactgtttc ggcgatgaag ctcgcgcgac cctcggcgtc
      241 gatgatctgc acgcgcagcg tcaccccgcc ggccagcggg ccgccggtcc ggatggccag
      301 cgcccgcagc aggcgctggg cgtcggccgc ctcacagccg ggcagcagcc gcaaccgcag
      361 gcagccggcg ggttcggccg gacgttcgat ctgggcgacc gccctgcgtc cggcaggacc
      421 agtcagcacg acgttctggc cccctgcgac ttcgatgtcg ccgccggcgg ccaggtcgag
      481 cgtggtcgcg gtgagggtcg aattgggcca gatcgatagc gatccgccgg ccaggtcccc
      541 cgcgtccgga tcgaagatgc gagcctcgtc gtcgatgacg tgcagcccgg ccaggtgcgg
      601 aatctcgcgg cgggccgacg agagcttcac cgacggcccg atcgcgatcg acggccacac
      661 cagggccagg ccgtccaggc tgctcgtcag gatgcggctg ccgtcgggcg agaagtgagc
      721 gctcgtcagt tcggcggcgt gccgcttcag actcagcact tccttgactt ccttgctcga
      781 ctccaggtcc cacagtttgg ccatgccgtc ctggctgccg gtgatcgccc gcagtccgtt
      841 gggcgagatg gccgcgctgg tcacggccgc cgtgtgacct tgcagcttga aggaccggac
      901 tggattgagg tcccagacat aagcgtcgtt gtcgttgcag ccggtgatga cccgcgtgcc
      961 gtcgggcgaa aacgcggcga acagcaccgc ctcgtcgtga gacagatgga ggggctcctg
     1021 cggcggctgg gcggcgatgt tccagacgcg ggcgaacttg tccttcgacg cagtgaggac
     1081 gaagttgccg tcgggcgaga attgggccga gtggacgcgg tcggtgtgcc gcagcacgtc
     1141 tccctccggt gtgatcgctc cgccggcgta gcgccacaag cgggccgtgc cgtcgttgcc
     1201 ggccgtaacc agccgatagc cgttgtcggc gatcacgggc gagaaggcga ccgagttcac
     1261 cgcggaccac tggctgccat tggagtgcgg atgggccagt ttgacggcga cgcggccgaa
     1321 ctccggatgc tggggatcgg cgtaccacag cttgacgtcg ccgtcgaggc tgctggtggc
     1381 gagcagcttg tcgtcgggcg agaactccgc cgaggtcact gcgccgtgcg agctgaatgt
     1441 cttttcgagt tggccctgcg ccgtgatcag gcgggcctgg ctgccgccgg cggccagcag
     1501 gtgcgagccg tcgggcgaga acgacgccgc ccagatcgcc ccccgcacct ggttctgcgg
     1561 ccacagcggc tggacttcgt tgcgggcgaa gtcccaccgc cagaaccggc tgctgttgcg
     1621 gttggcgctc gccgaagtca ggatcgcgct ggtctgcgcc gggtgaaaga cggcgctggt
     1681 cagggcttcg tcgggcagat tcacgtcgcg gaactgctcg ctgccgctag cgaggtccca
     1741 caggctaatc cggtagctgc ccttgcccgg cgacgagagc gacagcgccc ggcgcgaatc
     1801 ggcgctgatg tcgatcgcct tgacgccgcc ctgatgcttg agcggcggcg gcagcgtctg
     1861 gccggtcagc gcgtcgtgga tgagcacaat gcggtcctgc gacgccgtaa gcaggcggcg
     1921 gccgtcgggc gtgaacttca cggcggagat cggatagccc gtcgaatggc cgcggaccgg
     1981 cgtggcctgc cacgatcggc tgcccgcatc gaatcgccac agtcggacgc cgcccagcac
     2041 gtcgcccgtg gcgatccgca gagtctcgag gtcgcggtcg ggcgagaacg ccgccgccga
     2101 gacttcgccc ggatgccccg tcagcttgat cggttcgctc tgcgggtcgt tggcggacca
     2161 gagctgagcg ctttgcggct gccgcaattc ggcggcgtcg ctgccggtga cgatccactc
     2221 gccgtcggcc gacagagcca gcacgctgag cgtacccgtg ttccgcaggc ggcgcacctg
     2281 gccgccggtg cggcggtccc acatccgcac ggtgccgtcg ccggcgctgg tcagcacgtg
     2341 ccgatcgtcg ggcagaaacg ccacgctgct gaccaggaac tcgtgtccct ccttgatccg
     2401 ggcggcgggc gcggcggggc cggcggcgtc gagctcttcg ccttcgtcga acaccttgag
     2461 cgggcggcgc tcgccagcgt tccagacgag ggccttgtgg tcgcgaccgg ccgtcaccat
     2521 ccgctggccg ctccgcgaaa acgtcgccca cagcacggca tcctcgtggc cgcgcagcgc
     2581 tcggacttct tcatactcct gcgcgtccca gatcttcacg aagccgtcga ggctgctgga
     2641 gaccgcgtag cgcggatcgg ccgcgagcac gcagccgcgg acccagccgc cgtggccgcg
     2701 gaacgtcgtg gccaaatcga tggaggtcag attgggggca aatttccaga gtttgaccgt
     2761 gttgtcgttg ccgccgctgg ccagccacgt gccgtcggcc gaaaacgaca gggcccggac
     2821 ttcgcccgtg tgtcccgtca gggggggaaa ggtgggagtg tggaacgact gccgcggttc
     2881 cggcacgagc ttgcccagcc gttcctgctc cagctgcttg gcgatctggc caaagtttat
     2941 ctccagattc atgtcggccc gccagacgtg cacgtcgcgg tcgcggccgg ccgacgcgat
     3001 ccagttgccg cgcggtgccg ctgccacggc atagacgggg cctttgtgcc cgcggtagat
     3061 ccccttcttt ttgcccgtga ggctccagag ccgtacggtg ccgtcgtcgc cgcccgtgac
     3121 aaccgtgtcg ccgccggccg tgaatacggc cgaccagatc gggcccgtat gccccccctc
     3181 cgttggcacg ccctttcccg tctccagact ccacacttgt gcagtcccga cgcgcgagct
     3241 ggagactagc agcttgccgg acggcgagaa cgccaggctg agcacaccgc cagtgtcagg
     3301 gagcacggtc ttgctcgctt cgtcgtagct gccatccgcg gagcgctgat agagcgcaat
     3361 gccgccgtcg gcatcgcttg cagcggccaa ttggtcgccg ctggccgaaa gggcgacggc
     3421 cagcaccgga ccgcccaagt tgagcgttgg tttgggccgg aacgtgcact gcggaaagtc
     3481 gatctcccag acgaggactt tgcccgtagc cgtgccggcg acatagcggc tgccgtcggc
     3541 cgagcgggcg agcgattcga tgcgggtctt gacatcttgc tggccggcct gccggtcgca
     3601 gaggtgcttc agatagcccc attcccagtt ccggaagtac gaccagcgtg cggtggcgcc
     3661 ttggtagtcg ttaagcagct gatcggcgtc gaggaacgag ttgttggcga tccgctcggc
     3721 ggcgacgccg atttgggcgc ggtaggacgc agcggcggcg ttctgttcct cctggcgggc
     3781 ggtgcgggct tcgttcaggg cggcgacgcg cgtgaccttc aagtcctggt tcgtcgccag
     3841 caggtcggac ttcgacttga ggagcttttt ttccgactcc tgaacggtct tgatttcctc
     3901 atccagccgc ccgcgggcct gggtgaggtc cttgttcgtg gattccagcg acttgtactg
     3961 ctcggagatg gtgcggttct ggccgctgat gaaatacagg ccgacagaga gcgtgagcat
     4021 gaccagggcc gccagtccca gggccaggcg gcggagtccc ttgatgcggg cctggcgggc
     4081 ctcgcgctcg tggcgggcgg cggccagctt gccgtgcaag ccggcctgca cgggctgccg
     4141 ctcgtcgagc agggagaggc cgagatcgaa gtcgcctttt tcgagcgccc gctcggcata
     4201 ggccatccgg gcctcgattt cgccgctctt ggcccgctca ttgcccgacc agagagcgtg
     4261 cgcttcctga aaggcgaaca gcgcccgcga aaaatcctga tagtccttcg tctgccgggc
     4321 ttgctttagc tcttcatccg cccgcgtcga gagcacgacg ctctcagaat gcgactggta
     4381 ctcgcggacg gcggcctgga agtcctgcac gctggcgtag cggtcatccg gcttcgtagc
     4441 catcgcccgg taggccacgt cgagcaattc gccgctcttg tcggtgggct gaatctcgtt
     4501 gcgggccgcg gcatagaggc agctcatcac gtccttgccg gtgtgcggcg tgcggccggt
     4561 gacgatctcg aacagaaggg cgcccagcag gtagacgtcg ctgtgctgcc cgatcgcggt
     4621 cagcgggccg gtggccattt ccggtgccat gtaggccggc gtgccgccca tactggtcga
     4681 ttggccgatc ttgttcgagc ggatgaacgt ggccgtggaa agggcgatgc cccagtccat
     4741 gaccaggact tcgccgaagt cgccgagcat cacgttctcg ggcttcaggt cgcggtggac
     4801 cactccgcgg gagtgggcga aggcgatcgc gtcggccacg ctcatcagaa tccgcaggtt
     4861 ctcggccagc gactgttggc ggacgacatc ggaccagggc gtgccgcgaa cccgcttcat
     4921 cgcgtaaaac agcgagccca attcgctcgt gcccaggtcg tagatcggca cgatgtttgg
     4981 atggtcgagc tcgccggtga ccatcgcttc ggtcaggaac ttgttgcggt ggtcgcgctt
     5041 gtgggcgaat tcgtgccgca gcatcttgat cgccaccgtg cggtcaattg acgcctggcg
     5101 ggccgcatag accacgccca cgccgccttc gccgatcact tcctgcagct cgaactcgtt
     5161 cgtcgcctgc gtgatctccc cgggaagggc gatgcggaac gggcggatgt acagatcgct
     5221 gatcgccccc tcgggctcgg tcgatgaggc cttgatcgtc atcgacggtt tggtcgcgac
     5281 cgacgtgatc gacttcgacc agatccggtc cacttgctcg ggactggaac cttcgccgcc
     5341 ggcgggcggg ccggtgttca ccgtcttggt cgccgctggc gtcggcagcc gcgggcgcga
     5401 gggcggcgct gcgcccggag ctggggacag caggctcggc ggcggagaat cctcgtcttc
     5461 gtcgcccccg ccgcgcgaga ccagcgtgcg gatgatgtcg cgcatcgaca tcgcgtcttc
     5521 gtccaccggc ggagcggccg gcgcggagcc ttccggcgca gccggctgct gcgagctctc
     5581 ttcggcggag tcctgggccc agttcaaaat gctgccgcac tgcgggcagc ggccgccgcg
     5641 cagccggaag ctgccggttt cgctcaggtc cgcctcacaa aacgggcaac gcaacattcc
     5701 gtttctcctg cgcgccagaa gttcgcctgc cggctacgcg ctcttgcgcc accagcgcca
     5761 atgggcgggc gacgcgccga ttcgcgccgc cgcctgctcg acctgccctt tccagcgccc
     5821 cggcgagcgg ccggccgcga tggccgccgc ggccagcccg acgcccgcaa acagccgacc
     5881 cgcggcgggc tgctcggccg gcgtccagaa gtgctcggcc ggctgctggt cgtatcccag
     5941 cggcatcggc acatccgccg cagccggcag cggcgggagc gccatcgggc cggacgttcc
     6001 ctcgtgatgc tggtggggaa cgggggccgg cggcgccgcc ggaaccggcg gcagggccat
     6061 ttccggcgcg gcctcgtcgc ccgcctgctc caggggctcg accggcttgc cgtcgcggac
     6121 gagcacatcg atcaccagcc gctcctcggc gttgccggcc agcatcaaat agacgcgata
     6181 gcggtcgtcg ggaagccggc gaaacacggc gagcaaatcg gtgagcatgt ccgccgagag
     6241 gttcatgaca aactcttcgc ggaagctgcc gtcgccgttg ggcacgacca ggcggatttc
     6301 caggtaccgc agccgcaccg cctgcgtctt gaactccagc ggcggcggct gcgaaggcgg
     6361 cgtcagcggc ggcggcggtt gaaccggcgg ctgcagccgg accaggctcg taaagacggg
     6421 aatgagaatt gtcggcggct tgaaaatcac acgctggatc ggcggaacga gcgtcagctc
     6481 gccgatcagg aggccgggag tcggaacgtt gacgagcaaa cttcctgtga aattgccgac
     6541 cgtggccttg ataccgccgg ttctcttgtc gatcgagagc gggttgagcg ggttgtcgtc
     6601 gatcctgaac atgccctgca cgtttcccgt gaccagctgc aaaccctgaa tcgattgctg
     6661 caaggtggct ccgccccgca ggaccagtag cggaccgtcg ttgccgacag gttcgctcgg
     6721 gtcgtccgga tcgccgagcg tcagcgtgcc attgacgatc acctgatcgt agtccgtgcc
     6781 cgccgtttcg tatggcggat tgacgtcgaa caccatctgg ccggtgtcgg taaccgtgac
     6841 gttgccaaag atcgttagca cgctcgcgtc gccatcgatc gggttctccc gggtgcctgc
     6901 gggctcaaaa ttgccgggcg acaggacgcc ccggaccagc accaagttgg cggatatctt
     6961 cccgtcgccg cccagcaggg ccgcggtttc gacggtgacg gtttgagtgg cgaagatgaa
     7021 ccccgattcc gcgaccagga aatcgccgga ttggagcgac acttcgccga agctctggat
     7081 ggcctgcacg gtcgtgctgc cgtccaccgc gagatattgc gaaccggcag acaacgtccg
     7141 aagttgctgc gctgcaaaga aaatcggatt gtcggactcg ccaatgttgc ctgccgcgag
     7201 gagatgtaat tcgtcggcgg caatcttcgt atcgccgaag gtgatattcg agccggcgcc
     7261 gagggcagtc accgacagga agctcaacgt cctttgctgg aacgaatcgc cgtcgagttc
     7321 gatggaaccg gcgctggtgg acagtccgac ggtctggctc ccctgcacgc tgagcttggt
     7381 ggcatcgatg ccgcccccca cgacgttcac ccaggtgtcg ccgctggcga cgagagtcag
     7441 attgccggta ccgggcgcga cttgcagggg gt
//
LOCUS       DASRJB010000749         9385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1343127, whole genome shotgun sequence.
ACCESSION   DASRJB010000749 DASRJB010000000
VERSION     DASRJB010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343127"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFB80_19755"
     CDS             <1..311
                     /locus_tag="VFB80_19755"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN36080.1"
                     /translation="LVTEYPGKFAAAIPICGRGDPAAAERLAKTPIRVFVGGKDREQT
                     VQNCQEMTAALKKAGGDAELKLYPDLPHDCWTATYDEPAVWEWLLAQRLPEASAEPKK
                     "
     gene            341..1126
                     /locus_tag="VFB80_19760"
     CDS             341..1126
                     /locus_tag="VFB80_19760"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HZN36081.1"
                     /translation="MSTNPFAAPQTSPYPQSSTPAFKPIERLEYMRMYNYVFENPNWV
                     MNVLLTAVCTLIPVIGPLVLMGYQYEVMIALLMSGGARYPDFDFNRFADYLIRGLWPF
                     LVGLVASLVLVPLMMVLFIPMMLLFVGAANAGQDAGPFVVLIGMPILVLLNLAIVIVF
                     QMVLLPLQLRAGLAQDFGVAFNFQWIVNFAKKTWLEMVLGLLFVTFSYLVLAMLGFLV
                     FCVGVYAAVALFVLAQAHLLYQLYLLFLSRGGEPVQIKMSPAK"
     gene            1138..1944
                     /locus_tag="VFB80_19765"
     CDS             1138..1944
                     /locus_tag="VFB80_19765"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HZN36082.1"
                     /translation="MPDAKSAQTPVARGRIEYLRSVRIVFEHPQWMKGLLMLAVSMLI
                     PVLSTPVLFGYMYEVTEHLHLRQPGPYPLFEFRRFAHYITRGIWCYLLMNMLVAILVP
                     IVQVVFQGGFFASIAALQSRNNVAGLIVAVAVPAVLTAFFAFLLALSILLTPLYLRAG
                     LTHDFAQTFNFRWVGDFVRRMWLEVLLVNLFQWLASLVLLPLGCVMFCYGMLLAAALL
                     SISSGQLNWQLYELYLTRGGEPIPLRPVNVAHAASVGATNVSGDAGSVDY"
     gene            complement(1941..4046)
                     /gene="recG"
                     /locus_tag="VFB80_19770"
     CDS             complement(1941..4046)
                     /gene="recG"
                     /locus_tag="VFB80_19770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZN36083.1"
                     /translation="MSIAPAKSPAQQLATPVQYLKGVGPQRAELLARLDLHYAADVLF
                     NFPRAYQDMSELRSIDQLEEHKLASVAGVIDEVDLRNTGPGKSLLGMLVRQETRYLRC
                     LWFNQPWMRDKLQEGRRVLVSGEPVLEGMRWEMKHPRVELLADDEDAPAGRILPVYSL
                     TEGLNQSQLRRIVAGVVESHAALVEDVFPDEFLQQHNLWPIRTALPQIHEPADEASQQ
                     QAQRRFIYQELLVLQLALAIRKWRLSHQRQAPPLPTSSKIDARITRLFPFQLTTGQRQ
                     AIDEIAADMSRPFPMNRLLQGDVGSGKTVVAMYAMLLAVAHGYQAALMAPTEILARQH
                     LETLGRALAASKVRLTLLTGSLSATERQGVLEKIAAGEVDVVIGTHAVTHAVARSGVE
                     FKKLGLVVIDEQHKFGVRQRAALKKAGVDPHYLVMTATPIPRTVGMTLFGDLDVSTLR
                     EPPPGRQTVHTYLATDDQRMRWWHFFRSKLDEGRQGFVIAPLVEDSEAVEAANVQATF
                     DTLSRGELASYRVGLLHGRMTPAEKDSVMQAFRAGRTQVLIATSVVEVGVDVPNATLM
                     AIEGGERFGLAQLHQLRGRISRGSHPGYLCVFASPANDETQERLEAFTKTSDGFDLAE
                     LDFRLRGPGDLFGVKQHGLPPLRIADLARDVAVVEEARQDAQQLIATDPELASPAFAR
                     LRRMVFHRYGEALDLGDVG"
     gene            complement(4161..4532)
                     /locus_tag="VFB80_19775"
     CDS             complement(4161..4532)
                     /locus_tag="VFB80_19775"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN36084.1"
                     /translation="MFKQSAAFATACLLAGLASAQDAKTQFEKTVRPLFERKCFQCHS
                     SKADELKGNLKLESIELIKKGGDQGPAVVAGDKDSFLLRAIRYEVEDFQMPPSGRLED
                     KDIALIEKWVKALGDEAKAER"
     gene            complement(4548..5285)
                     /locus_tag="VFB80_19780"
     CDS             complement(4548..5285)
                     /locus_tag="VFB80_19780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN36085.1"
                     /translation="MPTDLTSKTALVTGGGSGIGLATAVALAREGCRVAIAGRRAEVL
                     REAAASWTAQPAIVHHSCDVADRTSVERLFAWADKQLGRIDILVNSAGVNIKNRLMSN
                     MEPPDWDRVLAINATGVYHCMYAVLPQMRARQDGLIVNISSISGKRAAAIGGVAYNAS
                     KFAVAALSTSVSNEECQNKIRITSIFPGEVDTPILEHRPQPVSAERRAKMLLPEDVAD
                     MVVALARLPPRAHVPELIIKPTIQEYV"
     gene            5454..6359
                     /locus_tag="VFB80_19785"
     CDS             5454..6359
                     /locus_tag="VFB80_19785"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN36086.1"
                     /translation="MGFRDKPLDATIHVVTPENIAFEYRLAGPFRRLPALLLDYCIRG
                     VVQVGIMIGLGLTLGFVSMGLAVFLIMLIWFVIEWFYGALFETFLNGQTPGKYLLGLR
                     VLSDNGRPIDGMQATLRNLLRVADMFGAVGLIAMLMNSKYKRLGDLVAGTIVVVEERQ
                     WLTGVAKLDDPRSIQLAAYLPPNFVVSRSMAKALATYVERRRYFTPPRRREVARHLAE
                     PLLERFGLPADTSYDLLLCALYYRTFIADRSQDERMLAEAQAAFVAANPFANLGTQIA
                     VQPKYVPPPLVQEQIVGAGFGGGRP"
     gene            6363..7400
                     /locus_tag="VFB80_19790"
     CDS             6363..7400
                     /locus_tag="VFB80_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZN36087.1"
                     /translation="MKVIDLLERRRQNWQELERLSDQVQGGGAPKMPPQSISRFGALY
                     RAACADLALAYAYQLPQNTVQYLHRLVGRAHNQLYRSRKFEWRMWAERLLFEVPQTIF
                     RDRCVQLMFCVFWGLFILSAWLAYAKTTWPEFAEQVLGAEMIDQLEKMYKDPITGREG
                     DLDMMMAGFYQRHNTSIGLQCFAWGLLVVPGMLVTISNAITLGASFGYMARPDVPEGE
                     NFFHFVTAHGPFELTAIVLSAGAGLRLGLSWMMPGELTRLSALVKAGREGMPVMGSAM
                     VMFFMAALIEGFLSPSAAPYWLKVFVAIVSSSLLAFYFVVLGFPRGLTMPTRKIEEPK
                     NQRTEEPKEKP"
     gene            complement(7449..8033)
                     /locus_tag="VFB80_19795"
     CDS             complement(7449..8033)
                     /locus_tag="VFB80_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36088.1"
                     /translation="MQVAAALAHRDRILLARPERVLFQQDDLVDVRPEAAHRRGPDQQ
                     HDDEEPLGEAVGLQPVHRQAQEPRRQDAAQEALAKQQLRRVAEHVAGDLLEAPFFEEQ
                     RRAEIRHDEDRGQRRFQIDQRRHAPPVAANVFERLHVRRRHPADERVVEQGQRHDRQQ
                     RRDQQRPGEIAGHVQRQIERVEQAPLADGDSGAV"
     gene            8371..9168
                     /locus_tag="VFB80_19800"
     CDS             8371..9168
                     /locus_tag="VFB80_19800"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZN36089.1"
                     /translation="MNISARIFVLLFAALSLAAGRLACAQDQASSDAQSVQDAKEILS
                     GSGRFPWYDRGKDDVRRLHAVPRDAADSRNRDSAWEKTPAAGGGGARPRFSLFGSALQ
                     WLGLTVLVVLLGVIAFLIAKTFLREEVSDQAVLRKVIESARDVDRVEALPFHVRKPSG
                     DFLSEARRLYEAGEYSEAIIYLFSYQLVQLDRQHLIRLAKGKTNRQYLRELRQRPHLR
                     AILDTTVVLFERAFFGRKTLTREQFEQAWRQLDDFHGELTITERAAA"
     gene            9165..>9385
                     /locus_tag="VFB80_19805"
     CDS             9165..>9385
                     /locus_tag="VFB80_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36090.1"
                     /translation="MSTAATSTGKEKAPSLLLPIAGIAAAVVVIGLGAWLAGRRDEQL
                     TTLYGRRRGTEAVRSVNGTSVLSELFKRKG"
BASE COUNT         1450 a         2988 c         3140 g         1807 t
ORIGIN      
        1 cgctcgtgac cgaatatccg ggcaagttcg ccgcggcgat tccgatctgc ggccggggcg
       61 acccggcggc ggccgagagg ctggcgaaga cgcccatccg cgtattcgtc ggcggcaaag
      121 accgcgagca aaccgtgcag aattgccagg agatgacggc cgccttgaaa aaggccggcg
      181 gcgatgccga actaaagctg tatccggacc tcccgcacga ctgctggacg gcgacctacg
      241 acgaaccggc cgtctgggag tggctgctgg cccagcggct gccggaggct tccgccgagc
      301 cgaagaaata gcgcagccgg ttcgctaatt caggaccaac atgtccacca acccgttcgc
      361 tgctccgcag acttcgccct atccccaaag ctcgacgccg gcgttcaagc cgatcgagcg
      421 gctcgagtac atgcggatgt acaactatgt gttcgagaat ccaaactggg tgatgaatgt
      481 cctgttgacc gccgtctgca cgctgattcc cgtgatcggc ccgctggtgc tgatgggcta
      541 tcagtacgag gtcatgattg ccctgctgat gagcggcggg gctcgctatc cggattttga
      601 ttttaaccgg ttcgcggatt acctgatccg cggcctctgg ccgttcctgg tgggactggt
      661 ggcgtcgctg gtgctggtgc cgctgatgat ggtcctgttt attcccatga tgctgctctt
      721 cgtcggcgca gcgaacgcgg gccaggatgc cggtcccttc gtggtgctga tcggcatgcc
      781 gatcctggtg ctgctcaacc tcgcgatcgt gatcgtgttt caaatggtcc tgctgccgct
      841 gcaactccgg gcgggtctgg cgcaggattt cggcgtggcc ttcaactttc aatggattgt
      901 caatttcgcc aaaaaaacct ggctggaaat ggtgctgggg ctgctgttcg ttacgttcag
      961 ctacctcgtg ctggcgatgc tgggcttcct ggtgttctgc gtcggcgttt acgccgccgt
     1021 ggccttgttc gtgctcgccc aggcacactt gctctatcag ctctacctgc tgttcctctc
     1081 ccgcggcggc gagccggtgc agatcaagat gtcgccggcg aagtagagcc acaacccatg
     1141 cccgatgcga agtccgccca gacgcccgtc gcccgcggac gcatcgagta tttgcgctcg
     1201 gtccggatcg tgttcgagca cccgcagtgg atgaagggcc tgctgatgct ggccgtcagc
     1261 atgctgatcc cggtgctcag tacgccggta ctgttcggct atatgtatga agtgactgag
     1321 cacctgcacc tgcggcagcc ggggccttat ccactgttcg agtttcgccg cttcgcccac
     1381 tacatcaccc gcggcatctg gtgctacctg ctgatgaaca tgctggtggc cattctcgtg
     1441 ccgatcgtgc aggtcgtgtt tcaaggcggc ttttttgcct cgatagcggc gctgcagtcg
     1501 cggaacaacg tggccgggct gatcgtcgcg gtagccgtgc ccgcggtgct gacggcgttc
     1561 ttcgcgttcc tcctggcgct gtcgatcttg ctcacgccgc tgtacctgcg ggcgggcctg
     1621 acgcacgact tcgcgcagac attcaacttc cgctgggtcg gagacttcgt gcggcggatg
     1681 tggctggaag tgctgctggt caacctgttc cagtggctgg cgtcgctggt cttgctgccg
     1741 ctgggctgcg tgatgttttg ttatggcatg ctgctggcgg cggccctgct cagcatctcc
     1801 agcgggcagc tgaactggca gctctacgag ctgtacctga cccgcggcgg cgagccgatc
     1861 ccgctgcggc cggtgaacgt agcccacgct gccagcgtcg gcgcaaccaa tgtttcgggc
     1921 gacgctggca gcgtcgatta ctagcccacg tcgcccagat cgagcgcttc gccgtagcgg
     1981 tggaagacca tgcggcggag gcgggcgaag gcgggactgg cgagctcggg atcggtcgcg
     2041 atgagctgct gggcgtcttg gcgggcttcc tcgacgacgg ccacgtcgcg ggcgaggtcg
     2101 gcgatccgca ggggcggcag gccgtgctgc ttgacgccga acaggtcgcc ggggccgcgg
     2161 agccggaaat cgagttcggc caggtcgaag ccgtcgctgg tcttggtgaa ggcctccagg
     2221 cgctcttgcg tctcgtcgtt agccggcgag gcgaagacgc aaaggtagcc gggatggctg
     2281 ccgcggctga ttcgcccgcg aagctggtgc agctgggcca ggccaaaccg ctccccgcct
     2341 tcgatggcca tcagcgtcgc gttaggcaca tcgacgccga cctcgaccac gctggtggcg
     2401 atcagcacct gcgtgcggcc ggcgcgaaag gcctgcatta ccgagtcctt ctcggccggc
     2461 gtcattcgcc cgtggagcag gcccacgcgg tagctggcca attcgccgcg gctgagcgtg
     2521 tcgaacgttg cctgcacgtt cgccgcctcg accgcctcgg agtcctcgac cagcggggcg
     2581 atgacgaagc cctggcggcc ttcgtcgagc ttgctgcgga aaaagtgcca ccaccgcatc
     2641 cgctggtcgt cggtggccag gtaggtgtgc accgtctgcc gccccggcgg cggctcacgg
     2701 agcgtcgaga cgtccaggtc gccgaacaag gtcatgccga ccgtccgcgg gatcggcgtg
     2761 gcggtcatca ccaggtagtg cggatcgacg ccggcctttt tcagggccgc ccgctgccgc
     2821 acgccgaact tatgctgctc gtcgattacc accagcccga gctttttgaa ctcgacgccc
     2881 gagcgggcga cggcgtgcgt gaccgcgtgc gtcccgatca cgacgtccac ttcgccggcg
     2941 gcgatcttct ccagcacgcc ctgccgctcg gtggccgaga gagagccggt gagcagcgtc
     3001 agccgcacct tgctggcggc cagagcccgg ccgagcgttt ccaggtgctg gcgggcgagg
     3061 atttccgtcg gggccattag cgcggcctgg tagccgtggg ccaccgccag cagcatcgcg
     3121 tacatggcga cgacggtctt gccgctgccg acgtcgcctt gcagcaggcg gttcatcggg
     3181 aacgggcgag acatgtccgc ggcgatctcg tcgatcgcct gccgctgacc ggtggtcagc
     3241 tggaacggaa agagccgcgt aatgcgggca tcgatcttgc tgctggtcgg cagcggcggg
     3301 gcctgccgct ggtgactcaa tcgccacttg cggatcgcca gcgcgagctg cagcaccagc
     3361 agctcctggt agatgaaccg ccgctgggcc tgctgctggc tggcttcgtc ggccggttcg
     3421 tgaatctgcg gcagggcggt gcggattggc cagaggttgt gctgctgcag gaactcgtcg
     3481 gggaagacgt cctcgaccag tgccgcgtgc gactccacga cgccggcgac gatccgccgc
     3541 agctgcgatt ggttcaggcc ttcggtcagc gaatagaccg ggaggatgcg gccggcggga
     3601 gcgtcctcgt cgtcggccag cagctcgacg cgggggtgct tcatctccca gcgcatgcct
     3661 tccagcacgg gctcgccgga gacgagcacc cgccggcctt cctgcagctt gtcgcgcatc
     3721 cagggctggt tgaaccacag gcagcggagg taccgcgttt cctgccgcac gagcatgccc
     3781 aggagcgatt tgcccgggcc ggtgttccgc agatcgacct cgtcgatgac gccggcgacg
     3841 ctcgccagct tgtgttcctc gagctggtcg attgagcgca gctcgctcat gtcctggtag
     3901 gcccgcggga aattaaagag cacgtccgcg gcgtagtgca ggtccaggcg agccagcaat
     3961 tccgcccgct gcgggccgac ccccttgaga tactgcaccg gcgtcgccag ctgctgggcg
     4021 ggagatttgg cgggtgcgat ggacatgcgc gtcgtcctaa tcgtattcgt aaccctaatc
     4081 ttaatcttcc cgttcactcc ggccggctca ggcggcagag cggcagacga ttaggattac
     4141 gatttagatt aggagggcaa ttatctttct gctttcgctt cgtcgccgag ggccttcacc
     4201 cacttctcga tcagcgcgat gtccttgtct tccagccggc cgctgggggg catctgaaaa
     4261 tcctcgacct cgtagcggat ggcccgcagg aggaagctgt ccttgtcgcc ggcgacgacg
     4321 gcggggccct ggtcgccgcc cttcttgatc agctcgatcg attccagctt gaggttgccc
     4381 ttcagctcgt cggctttcga cgagtggcac tgaaagcact tgcgctcgaa gagcgggcgg
     4441 acggtctttt cgaattgggt cttggcatcc tgggcggaag ccaagcccgc gagcaggcac
     4501 gcagtggcga aggcggcgct ctgtttgaac atcggattcg ggcttcgtca aacgtactct
     4561 tggatcgtcg gcttgatgat caactccggc acatgcgctc gcggcggcag cctggccagg
     4621 gcgacgacca tgtcggccac gtcctcgggc agcagcatct tggctcgccg ttcggcactg
     4681 acgggctgcg ggcgatgctc gaggatcggc gtatcgacct cgcccgggaa gatgctggtg
     4741 atgcgaatct tgttctggca ctcctcgttg ctgacgctgg tgctgagggc ggcgacggcg
     4801 aacttggagg cattataggc tacgccgccg atggccgccg cccgcttgcc cgagatcgac
     4861 gagatgttga cgatcaggcc gtcctggcgg gcccgcatct gcggcagcac ggcgtacatg
     4921 cagtgataga cgccggtggc gttgatcgcc agcactcggt cccagtcggg cggctccatg
     4981 ttcgacatca gccggttctt gatgttcacg ccggccgagt tcacgaggat gtcgatccgc
     5041 ccgagctgct tgtcggccca ggcgaagagc cgctcgacgc tcgtccggtc agccacgtcg
     5101 cagctgtgat gcacgatcgc cggctgagcg gtccaactcg ctgccgcctc gcgcagcacc
     5161 tcggcccgcc ggcccgcgat cgccacgcgg cagccttcgc gggccagcgc cacggccgtc
     5221 gccagcccaa tgccgctgcc gccgccggtg acgagggccg tcttggaagt taggtcagtt
     5281 ggcattggat caatttcaga ttgcagattt cagattgcag attggagaga gagtttatcc
     5341 gcgcgcgggc tgggggaaaa ggttcgcgcc tcgcacgctt gcgacgcggc gggctgcggc
     5401 gaataatgca gttgcctctt cgccggcgaa tacctcctag acgcgcgccc cgcatgggct
     5461 ttcgagacaa accgctcgac gcgacgatcc acgtcgtcac gcccgagaac atcgccttcg
     5521 agtatcgcct ggcgggtccg tttcgccggc tgccggcgct gctcctggac tattgcatcc
     5581 gcggcgtggt gcaggtgggc atcatgatcg gactggggct gacgctgggc ttcgtcagca
     5641 tggggttggc ggtgttcctg atcatgctga tctggttcgt gatcgagtgg ttctacggcg
     5701 ccctgttcga gacattcctc aacggccaga cgccgggcaa gtacctgctg ggcctccgcg
     5761 tgctgagcga caacggccgc ccgatcgacg gcatgcaggc gacgctccgc aatctgctgc
     5821 gggtggccga catgttcggc gcggtcgggc tgatcgcgat gctgatgaac tccaagtaca
     5881 agcggctggg cgacctggtc gccgggacga tcgtcgtcgt cgaagagcgg cagtggctca
     5941 ctggcgtggc gaagctcgac gatccgcggt cgatccagct cgcggcgtac ctgccgccga
     6001 atttcgtcgt cagccgctcg atggccaaag ccctggcgac ctatgtcgag cggcggcggt
     6061 atttcacgcc gccgcggcgg cgcgaagtcg cgcggcacct ggccgagccg ctgctggagc
     6121 gattcggatt gccggccgac acgagctacg acctgctgtt gtgtgccttg tactaccgca
     6181 cgttcatcgc cgaccgcagc caggatgagc gaatgctggc cgaggcccag gcggccttcg
     6241 tcgcggcgaa tccgttcgcc aacctgggta cgcagattgc cgtgcaaccg aagtacgtgc
     6301 cgccgccgct ggtgcaggag caaatcgtgg gagcggggtt tggaggaggc agaccgtagc
     6361 ctatgaaagt catcgacctt ttggaacgcc gccgccagaa ttggcaggag ctggagcgcc
     6421 tcagcgacca ggtgcaaggc ggcggcgcgc ccaagatgcc gccgcagagc atctcgcggt
     6481 tcggggcgct gtatcgcgcg gcctgcgccg acctggccct ggcctacgcc tatcagctgc
     6541 cgcagaacac cgtgcagtat ctgcaccgcc tcgtcggccg cgcccacaac cagctctatc
     6601 gcagccgaaa gttcgagtgg cggatgtggg ccgagcggct cttgttcgaa gttccgcaga
     6661 cgatctttcg cgaccgctgc gtgcagctga tgttctgcgt gttttggggt ctgttcatcc
     6721 tctcagcctg gctggcctat gcgaagacga cctggcccga attcgccgag caggtgctcg
     6781 gcgcggagat gatcgaccag ttggaaaaga tgtacaagga cccgatcacc ggccgcgagg
     6841 gggacctcga catgatgatg gccgggttct atcagcggca caacacgagt atcggcttgc
     6901 agtgctttgc ctggggactg ctcgtcgtgc cgggaatgct ggtgacgatc tccaacgcga
     6961 tcacgctcgg cgcgtcgttt ggttacatgg cccgccccga tgtacccgag ggggaaaact
     7021 tcttccactt cgtcaccgcc cacgggccgt tcgagctgac cgcgatcgtg ctctcggccg
     7081 gggcgggctt gcggctgggg ctgtcgtgga tgatgccggg agagctgacg cgactgtcgg
     7141 cgctggtgaa ggccggccgc gaaggaatgc ccgtcatggg ctcagcgatg gtgatgttct
     7201 tcatggccgc gctgatcgag ggcttcctct cgccgtcggc cgcgccgtat tggctcaagg
     7261 tttttgtcgc gatcgtctcc agcagcttgc tggcgttcta ttttgtagtc ttggggttcc
     7321 cgcgggggtt gacgatgccg actcgaaaaa tcgaagaacc gaagaaccaa agaaccgaag
     7381 aaccgaagga gaaaccataa cgtgtccctt ttggttcttt ggttcttcgg ttcttgagat
     7441 ttatgcagtt agaccgcacc agaatcgcca tccgcgagcg gggcctgctc gacacgctcg
     7501 atctggcgct gcacgtgacc cgcgatttcg ccgggccgct gttggtcgcg tcgctgctgg
     7561 cgatcgtgcc gctggccctg ctcaactacg cgctcgtcgg ctggatggcg ccggcggaca
     7621 tggagtcgct cgaagacgtt ggccgctact ggtggagcat gacggcgctg atctatctgg
     7681 aagcgccgct ggcctcgatc ttcgtcgtgg cgtatctcgg cccggcgttg ttcctcgaag
     7741 aacggagctt caagcagatc gccggcgacg tgctccgcta ctcgccgcag ctgctgcttt
     7801 gccagggcct cttgcgcggc gtcctgccgg cgtggctctt gtgcctgacg gtggaccggc
     7861 tgcaggccaa cggcttcacc gaggggttcc tcgtcgtcgt gctgctgatc tggtccacgg
     7921 cgatgcgcgg cttccggcct tacatcaacg agatcatcct gctggaaaag aacccgctct
     7981 ggtcgagcca gcaaaatacg atcacgatgg gcaagcgcag cagccacctg catggcccgt
     8041 attcggcgga cctgctgatc cgctggttcg gggcagcggc gatcgccctc ttgctgctgg
     8101 ccttgacgct gctgaccgcg gtcgtcgtac agggcgtgct catcagcgac tggcccgtcg
     8161 tgggcgtcat gaccgaggac tttgcctttc ggctggattg gttcaagctg cagatcgtct
     8221 accccgcgtg cctgtggttc gtcgtggcct tcatgtcagt ggtgcggttt ttgagctacc
     8281 tcgacttgcg gattcgccac gaaggctgga agtcgagttg ttcatgcggg ccgaggccct
     8341 ccgcctcgcc gcgcggatgg agtaagtgga ttgaacatat cggcgcgtat cttcgttctc
     8401 ctattcgcgg cgctgtcgct cgccgctggc cgcctggcgt gcgcccaaga ccaggccagc
     8461 tccgatgccc agtcggtgca ggacgccaag gaaatcctct ccggcagcgg acggttcccc
     8521 tggtacgacc gcggaaagga cgacgtgcgg cggctgcacg ccgtgccccg cgacgcggcc
     8581 gactcccgca accgcgactc ggcctgggag aagacgccag cggcgggcgg cggcggagcc
     8641 cggccgcggt tcagcctgtt cggctcggcg ctgcagtggc tggggctgac ggtgctggtc
     8701 gtgctgctgg gagtgattgc cttcctgatc gccaagacgt ttctccgcga agaggtcagc
     8761 gaccaggccg tgctccgcaa ggtcatcgag agcgcccgcg acgtcgatcg cgtcgaggcc
     8821 ctgccgtttc acgtccgcaa gcccagcggc gatttcctca gcgaggcccg ccggctgtac
     8881 gaggccggcg aatactccga ggcgatcatc tacttgttca gctaccagct ggtgcagctc
     8941 gaccggcagc acctcatccg gctggccaag ggcaagacca accggcaata cctccgcgag
     9001 ctgcggcagc ggccgcactt gcgggcgatt ctcgatacga cggtcgtgct cttcgagcgg
     9061 gcgttcttcg gccgcaagac gctgacccgc gagcagttcg agcaggcctg gcggcagctc
     9121 gacgatttcc acggcgaact cacaatcacg gagcgcgccg ccgcatgagc accgccgcaa
     9181 cgtccaccgg caaagagaag gcgccctcgc tgctgctgcc gattgccggc atcgccgccg
     9241 cggtggtggt gatcggcctg ggggcctggc tggcaggccg ccgcgacgag cagctgacga
     9301 cgctctacgg ccgccgccgc ggcaccgagg ccgtccgcag cgtcaacggc acctcagtgc
     9361 tcagcgagct gttcaagcgc aaggg
//
LOCUS       DASRJB010000750         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3883603, whole genome shotgun sequence.
ACCESSION   DASRJB010000750 DASRJB010000000
VERSION     DASRJB010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3883603"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFB80_19810"
     CDS             complement(<1..220)
                     /locus_tag="VFB80_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36091.1"
                     /translation="MRVAAGGLTCVLALGFVTALAAEEGASPWTAVVRPGGHANCFVA
                     AESDLPRVDRAGREALLDYPATETTIGAP"
     gene            complement(397..1128)
                     /locus_tag="VFB80_19815"
     CDS             complement(397..1128)
                     /locus_tag="VFB80_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36092.1"
                     /translation="MKTACLAIAALLLASSAHAADPMPPDGFRPLFNGRDLTGWYGLN
                     PHSVVKLEGEKREAMLKQMRDDFANHWRVENGELVNSGTGPYATTAEEFGDSELLIEY
                     KTVAKADSGIYLRGTPQVQIWDYTKEGGKWDRNADKGSGGLFNNAKGTPGQLPLVLAD
                     KAFGEWNAFRIRQAGAKTWVWLNDKLVVDGAVMENFWDRSKPLPAKGPIMLQTHGGEI
                     RWRNIFLREIGAGEAAKLLAEKPGN"
     gene            complement(1190..2671)
                     /locus_tag="VFB80_19820"
     CDS             complement(1190..2671)
                     /locus_tag="VFB80_19820"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN36093.1"
                     /translation="MPQLIPTGEATAILPTGGETKPITVIGTEPIRSTFDELCLQQAV
                     NSRLAPGVSEVVLNPDGHCGYGAPIGCVLVSPTHVYPGPVGVDIKCSMSLLQLDLPAD
                     QIEDRRTRRELINAVCRRIPTGAGRGQRNVKNGRRISEALGKQLLVEGASADVCTQLG
                     VPPEWAQRCEDWRHLGHDERAGSLGERLEWHLEAKTLRDFGAKVSQLGSYGGGNHFGE
                     CEVVHVENTPRARETARVFGLADGKVAFLSHCGSRGVGHNLAMGQFGSLQRKFARWDI
                     PLPGNDRELVYAPLGTPEADAYLDDMALGANFATLNHLLINALVLEAFAEVMPGVRGE
                     LVYFISHNIARKEIVNNVPAWVHRKGATRAFPAGHHALKDTNYADTGHPILLPGNPQA
                     GSAVMVADAGASLSCYSVNHGAGRVLGRKRAIRELDQAAIDQSFDQHDILTNCRQYPK
                     DEAPAAYKNFDEVLRSVKTAGLASEVARLKARFVIKDGDAADD"
     gene            2881..4128
                     /locus_tag="VFB80_19825"
     CDS             2881..4128
                     /locus_tag="VFB80_19825"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZN36094.1"
                     /translation="MKSRMLALAAVLTLAASAAAGDLLQCFSDLASGDFAARQRATQD
                     LQAAGPEAIPLLAQALRSSDREVRSRSIDILLSHALSPQYERREPALRTLREIAAGGD
                     SPTAEVAQGTLEQVRGVTVSLAANELTRLGATVMPVENSEPQQYNIQIRQSWTGGDER
                     LALLLDLAAVPWLSLENAPVSDAALAYVAKLRSLDKLYLGSSRITGSGLRQLAPLANL
                     QYLSLKQLPIDDEKLRALPRFPNLRYLGLDGTRITDAGLAELERYPQLQTLWLDNTPI
                     TDAGLAHLKPLKSLRTLFLPGTQTAGPGLAALRNLPSLTYLSLKGTKQSSVSLKLVGQ
                     LDQLETLGLDQTNITDEQLESLAGLTKLRILWLSNTEITDAGLEPLKKLKNLQVLYLT
                     ATQVTSDGVADLARALPTCHVAR"
     gene            complement(4196..5629)
                     /locus_tag="VFB80_19830"
     CDS             complement(4196..5629)
                     /locus_tag="VFB80_19830"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HZN36095.1"
                     /translation="MIKRWAWMLPVLSSVATFVAAPARAQDYPADPAAMAAMQAQYQA
                     YMAQAQYAAQAQYAAPYGYPPGMIPAGYMQDPTAAPMPGGAPGPMPDVYGSYGSMPMD
                     YGGQMDGGFAMGGCSFCGGAGCQACAGCGGGHHGDHGLLGDVFGCVGPYPDGGCGAVR
                     WFDFAVDFMYLRRDDPGRNVDLTSQGINGPIVLRTGQLDLEEAAGFRFIGCMQVGPGG
                     NVEFVYFGQFHWDDGAFVRRSGFNDLFSVFSQFGDVPFNGFAETDRSDFQRIDYTSEF
                     DSFEINFRQRWMAPNCRYQGSWVCGVRHFILDEKFRLFTSSSVNGINGDPLNPAQARF
                     DVDTTNNLTGFQLGGDMWICVLPGLRLGGEIKAGVYGNHMNANTTIGVNNGNIPTFTE
                     RVSINDVAFIGQADLTATYRINYNWTARIGYQFLFVDGVALAGENFNTLPPALFVPPP
                     GATRVPTINDNGNVFYHGWTAGLEFMW"
     gene            complement(5851..6768)
                     /locus_tag="VFB80_19835"
     CDS             complement(5851..6768)
                     /locus_tag="VFB80_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36096.1"
                     /translation="MNVVRRPAFAQTIERALRSFDVEPIRVLPLLTVVLLLTSAPAQW
                     YLVGPLVALFALGVVYRRWLARPAFWYVVATLLGTTVYLNWESSDNHKYLFVYWCLAL
                     TSALSLPREQQAPALAQSSRWLIGLCMLLAVLWKVSTPEYCSGSFFHYTLLADDRFAQ
                     FTSLAAGVPLDQLRDNAKLVDLLREGHLTAGTSIASVVLASSPAVNLLALAMTWWTVA
                     IEGLLAVLYLAPNGRRLAASRNLLLVLFAATTYLIAPVRGFGWMLMLLGLAQCEDRDR
                     IFRPLFLAALVLIQIYTLPFATVVQRLTQ"
     gene            complement(6765..>7059)
                     /locus_tag="VFB80_19840"
     CDS             complement(6765..>7059)
                     /locus_tag="VFB80_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36097.1"
                     /translation="ISQRDGLEISAAALRCPSSVTASPLAAGQSHALEPLPRSEPSLS
                     LPLGAGRGEGLSEAIPFTAVRLECWRGRFDAATDLLSAERLLSVTVTREEARP"
BASE COUNT         1227 a         2285 c         2358 g         1189 t
ORIGIN      
        1 acggcgcacc gatcgtcgtt tccgtcgccg gataatcgag cagcgcctcg cggccggccc
       61 gatcgacgcg gggcagatcg ctctccgccg cgacgaagca gttggcatgc ccgcccggcc
      121 gcacgaccgc cgtccatggg cttgcgcctt cctcggccgc cagcgccgtg acaaatccca
      181 gtgccagcac acaagtcaaa ccaccggcgg caactcgcat gggacgactc tcgcgcacga
      241 gcttcaggtg aaaaggagac ctgaactctc tagcgcgaaa cggccctggt ttgttacagc
      301 agcattagag ccgcggataa acgcggatga cacggatcgg agagacaagt cgagtgaatt
      361 ccctgccgat ccgtgttaat ccgtgttcat ccgtggctaa ttccctggct tttcagcgag
      421 gagcttggcc gcttcgcccg cgccgatttc acggaggaag atgttccgcc agcgaatttc
      481 gccgccgtgg gtctggagca tgatcggccc cttggcgggc agcggcttcg aacggtccca
      541 gaagttctcc attaccgcgc cgtccacgac cagcttatcg ttcagccaga cccaggtttt
      601 tgctcccgct tgccggatgc ggaacgcgtt ccactcaccg aaggccttgt cggccaggac
      661 cagcggcaat tgacccggcg tgcccttggc gttgttgaac agtccgcccg agcccttgtc
      721 ggcgttgcgg tcccacttgc cgccctcctt ggtgtaatcc cagatttgca cctgcggcgt
      781 gccgcgcagg tagatgccgc tgtcggcctt ggcgaccgtc ttgtactcga tgagcagctc
      841 gctgtcgccg aactcctcgg ccgtcgtggc gtacggcccg gtgccgctgt tgaccagttc
      901 gccgttctcg acccgccaat ggttggcaaa gtcgtcgcgc atctgcttca gcatcgcctc
      961 gcgtttctcc ccctccagct tcacgaccga gtgcggattc aggccgtacc agcccgtcag
     1021 gtcgcggccg ttgaacagcg ggcggaagcc gtccggcggc atcgggtcgg cggcgtgggc
     1081 ggaagaggcg agtagtagcg cagctatcgc gaggcaagca gtcttcatgg gagttttccg
     1141 attggaggaa cactccctgc gcgacggcac acggagtgtg cctactactt tagtcgtccg
     1201 cggcgtcgcc gtctttaatg acgaagcggg ccttgaggcg cgcgacctcg ctggccaggc
     1261 cggcggtttt caccgagcgg aggacctcgt cgaagttctt gtacgccgcc ggggcctcgt
     1321 ccttgggata ctggcggcag ttggtcagga tgtcgtgctg gtcgaacgac tggtcgatgg
     1381 ctgcctggtc gagctcgcgg atcgcccgtt tgcggcccag cacgcggccc gcgccgtggt
     1441 tgacgctgta gcaggacagc gaggccccgg cgtcggcgac catcacggcc gagccggcct
     1501 gcgggttgcc cggcagcagg atcgggtgac ccgtgtcagc gtagttcgtg tctttcagcg
     1561 cgtgatggcc ggcgggaaac gcccgcgttg cccctttgcg gtggacccag gccggcacgt
     1621 tattcacgat ctccttgcgg gcgatattgt ggctgatgaa atagaccaac tccccgcgca
     1681 cgcccggcat cacctccgca aacgcctcca ggaccagcgc gttgatgagc aggtgattga
     1741 gcgtggcgaa attcgctccc agggccatgt cgtcgaggta cgcgtcggct tccggcgtgc
     1801 ccagcggagc atagaccagc tcgcggtcat tgcccggcag cgggatgtcc cagcgggcga
     1861 acttgcgctg cagcgagccg aactgcccca tggccaggtt gtgcccgacg cctcgcgaac
     1921 cgcagtgcga gaggaacgcg actttgccgt cggccaggcc gaacactcgc gcggtctcgc
     1981 gggcgcgggg cgtattctcg acgtggacca cttcacactc gccaaagtgg ttgccgccgc
     2041 cgtacgagcc gagctggctg accttcgcgc cgaaatcgcg cagcgtcttg gcttccaggt
     2101 gccactccaa tcgctcgccg agcgaccccg cccgctcgtc gtggcccagg tgccgccaat
     2161 cctcgcaccg ctgggcccac tcgggtggaa cgccgagctg cgtgcagacg tcggccgatg
     2221 cgccctcaac cagtaactgc ttgccgaggg cctcggaaat gcggcggccg ttcttcacgt
     2281 tccgctgccc gcgccccgcg ccggtcggaa ttcgccggca gacggcgttg atcagctccc
     2341 gccgcgtgcg gcggtcctcg atctggtccg ccggcaaatc gagctgcaag aggctcatgg
     2401 agcacttgat atcgacgccg accggcccgg gataaacgtg cgtcggcgaa acgagcacgc
     2461 agccgatcgg cgcgccgtag ccgcagtgcc cgtcagggtt cagcacgacc tcgctgacgc
     2521 ccggcgctag ccgcgagttg accgcttgct ggaggcacag ttcgtcgaac gtgctgcgga
     2581 tcggctcggt gccgatcacg gtgatcggct tcgtctcgcc gcccgtcggc agaatcgccg
     2641 tggcttcgcc ggtgggaatg agctggggca tgccgtcctt tgtagccgaa ttcgccagaa
     2701 ttcggtttct gaatttggga tttgggattt ggaaaacacg acgacgctct attcagacag
     2761 tacgccgctg aatgggttct cgcctccttg cagcggttgg catttttcct cgtgtttttc
     2821 cagcggcgag cggataggat ggcgggcggg gattctgacg gaattgcgga gcggcaggcg
     2881 atgaagagcc gaatgctggc actggcggca gtattgacgc tcgcggcatc ggccgctgcc
     2941 ggcgatttac tgcagtgctt ttccgatctg gccagcggcg acttcgccgc gcggcagcgg
     3001 gcgacgcagg atttgcaggc cgcgggtccg gaggcgatcc cgctcttggc ccaggcgctg
     3061 cgctccagcg accgcgaagt ccgcagccgg tcgatcgaca tcttgctctc gcacgccctc
     3121 tcgccgcagt acgagcgccg cgagccggcc ctgcggacgc tgcgggaaat cgcagcgggc
     3181 ggcgactcgc ccaccgcgga agtggcacaa ggaactctgg agcaggtccg cggagtcacc
     3241 gtctcgctgg ccgccaacga actgacgcgg ttgggcgcga ccgtaatgcc ggtcgaaaac
     3301 tccgagccac agcaatataa catccagatt cgccagagct ggaccggcgg cgacgagcgg
     3361 ctggcgctgc tcttggactt ggccgcagtg ccctggctgt cgctcgagaa cgctccggtc
     3421 agcgacgctg cgctggccta tgtcgcgaag ctgcggagcc tcgacaagct gtacctgggc
     3481 agcagccgca tcacgggcag cggccttcgg cagctcgcgc cgctggcgaa cttgcaatac
     3541 ctctcgctca agcagctgcc catcgacgat gagaagctgc gggccctgcc gagatttccc
     3601 aacttgaggt acctgggcct cgatggcacg cggatcaccg acgcgggcct ggcagagctg
     3661 gagcgctatc cgcagctgca aacgctgtgg ctcgacaaca cgccgatcac cgatgcgggc
     3721 ctggcgcacc tgaagccgct caaaagcttg cggactctgt ttctgccggg tacgcagacc
     3781 gccgggccgg gcctggccgc gttgcggaac ctgccgagcc tgacgtatct gtcgctcaag
     3841 ggcacgaagc agagcagcgt gagcctgaag cttgtcgggc agctggacca gctggagacg
     3901 ttggggctgg accagacgaa catcaccgac gaacagcttg agtcgctggc aggtctgacg
     3961 aagctgcgga ttttgtggct gtcaaacacc gagattacgg atgccggcct ggagcccctg
     4021 aagaagctca agaacttgca agtcctctat ctcaccgcca cccaggtcac cagcgacggc
     4081 gtcgccgacc tggcccgcgc gctgcccacc tgccacgttg cccgctaacg ggtgctgtgg
     4141 cagcgccgct tcccaatgca gccggccctg gaggccgggg ctagccggca ctctgctacc
     4201 acatgaattc caagccggcg gtccagccgt ggtagaagac gttgccgttg tcgttgatcg
     4261 tcgggacgcg ggtcgcgccg ggcggcggaa cgaacagggc cggcggcagc gtgttgaagt
     4321 tctcgcccgc cagggccacg ccatcgacga acaggaactg atagccgatg cgggcggtcc
     4381 agttatagtt gatccggtac gtcgcggtca ggtcggcctg gccgatgaac gccacgtcgt
     4441 tgatcgagac ccgctcggtg aacgtcggga tgttgccgtt gttcacgccg atggtcgtgt
     4501 tggcgttcat gtggttgccg tacacgcccg cctttatctc gccgcccaga cgcagcccgg
     4561 gcaacacgca aatccacatg tcgccgccga gctgaaagcc cgtcaggttg ttggtcgtat
     4621 cgacgtcgaa gcgggcttgg gccggattga gcggatcgcc gttgatgccg ttgaccgacg
     4681 agctggtgaa cagccggaac ttctcgtcca ggatgaagtg cctgacgccg cagacccacg
     4741 agccctgata gcggcagttg ggggccatcc accgctgccg gaagttgatt tcaaagctgt
     4801 cgaattccga cgtgtagtcg atccgctgga agtcagagcg gtcggtctcg gcgaagccgt
     4861 tgaagggcac gtcgccgaac tggctgaaga ccgagaacag atcgttgaag ccggagcggc
     4921 ggacgaacgc gccgtcgtcc cagtgaaact ggccgaaata gacgaattcc acgttgccgc
     4981 cggggccgac ctgcatgcag ccaatgaagc ggaagcccgc ggcttcttcc agatcgagct
     5041 ggcccgttcg taggacgatc ggaccgttga tgccttgcga ggtcaggtcc acgttgcggc
     5101 ccggatcgtc gcgccgcagg tacataaagt ccacggcgaa gtcgaaccac cgcaccgcgc
     5161 cgcagccacc gtcgggatag ggcccgacgc agccgaacac atcgcccaag aggccgtggt
     5221 cgccgtgatg tccaccgccg cagccggcgc aggcctggca tccggcgccg ccgcagaagg
     5281 agcagccgcc catcgcgaag ccgccgtcca tttgcccgcc atagtccatc ggcatcgacc
     5341 cgtacgagcc gtagacgtcg ggcattggac cgggcgcacc gccgggcatc ggggccgccg
     5401 ttggatcctg catatagccc gccggaatca tgccgggcgg atagccgtac ggagctgcgt
     5461 attgggcctg cgcggcgtac tgagcctggg ccatatacgc ctgatactgc gcctgcatgg
     5521 ccgccatggc cgccggatcg gctggatagt cctgcgcccg agccggcgcg gcgacgaagg
     5581 tcgcaacgga cgacagcacg ggcagcatcc acgcccaacg cttaatcatc ggacagtctc
     5641 cgcggctcca aagttggttc gccaggcagt agcgaccgtt agcgcctccc gccgctggca
     5701 gcggggggaa tacgagccga ccgcagcgcc ttttttcgct cgactacacc tatcggcgcg
     5761 gaataacggt caaaatcagt actttcgtca taaccggtaa ttttcccgga aatcgcattg
     5821 tcggggcagt ttgcccaggt tgacgtgtcc ctactgcgtc agccgctgga ccacggtggc
     5881 aaaggggagc gtgtagatct ggatcagcac gagcgccgcc aggaacagcg gccggaagat
     5941 gcggtcgcgg tcctcgcatt gggccaggcc cagcagcatc agcatccagc cgaagccgcg
     6001 gactggggcg attaagtaag tcgtggccgc aaagaggacg agcagcaggt tgcggctggc
     6061 ggccaggcgg cggccattgg gtgccaggta cagcactgcc aggaggcctt cgatggcgac
     6121 ggtccaccag gtcatcgcta gcgcgagcag gttgaccgcg ggcgagctgg ctagcacgac
     6181 gctggcgatg gacgttccag cggtcaaatg gccctcgcgc agcaagtcca cgagcttcgc
     6241 gttgtcgcgg agctgatcga gcggcacgcc ggcggccagg ctggtgaact gggcgaagcg
     6301 gtcgtcggcc aggagcgtgt agtggaagaa ggagcccgag caatactcgg gcgtcgagac
     6361 tttccacagc actgccagca gcatgcacag gccgatcagc cagcggctgc tctgagccag
     6421 cgccggcgct tgctgctcgc ggggcagcga gagggcgctc gtcagggcca ggcaccaata
     6481 gacgaacagg tatttgtggt tgtcggaaga ttcccagttg agatagacgg tcgtgcccag
     6541 gagcgtcgcc acgacgtacc agaatgcggg ccgcgccagc cagcggcgat agacgacgcc
     6601 cagggcaaag agcgcgacca gcggaccgac caggtaccac tgcgccggcg cgctggtgag
     6661 cagcagcacg accgtgagca gcggcaggac gcggatcggc tcaacgtcga agctgcggag
     6721 agctcgttcg atggtttgtg caaaggccgg gcgccgaacg acgttcatgg ccgggcctcc
     6781 tcgcgcgtca cggtaacgct cagcagccgc tcggcgctga gcaagtcggt cgcggcgtcg
     6841 aaccgcccgc gccagcactc gaggcggacg gcggtgaagg ggatggcctc ggatagaccc
     6901 tcaccccggc ccgctcccag agggagagag agcgagggct cgctccgcgg gagcggttcc
     6961 agcgcgtgcg attggccggc ggcgagcggg gatgcggtca cggaactcgg acaacggagg
     7021 gccgccgcgg agatctctag cccatcgcgc tggctgatc
//
LOCUS       DASRJB010000751         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1068573, whole genome shotgun sequence.
ACCESSION   DASRJB010000751 DASRJB010000000
VERSION     DASRJB010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068573"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFB80_19845"
     CDS             complement(<1..363)
                     /locus_tag="VFB80_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36098.1"
                     /translation="MTADDTGEALLKQALAGDIPALAALFSQHRDRLKRMVKLRLDRR
                     LQGRVDASDVLQEAYLDLAQRLPEYGREPKFPLYLWMRLVTGQRLAQVHRKHLGAAMR
                     NAELEVSIYQKALPQASTE"
     gene            complement(489..1982)
                     /locus_tag="VFB80_19850"
     CDS             complement(489..1982)
                     /locus_tag="VFB80_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36099.1"
                     /translation="MNRFALGLFVVVLTLARASAAPPNIVLLMGDDHGWEETGYHGHP
                     HVKTPELDKMAAAGLRMDRFYAAHPNCSPTRGSFLTGRHPNRYGTFAPGWSFRPEEIT
                     IAQILRPAGYRCGHFGKWHVGPVKAASPVCPGAMGFHEWLSHDNFFELNPSLSRNGGP
                     PEVIAGESSEILVREAIRFIDGVRAESKPFFTIVWFGSPHEPYSGLPADLALYDDLPA
                     KYAEKKVRLTSNETGGQVTRPQGEVLRERYAEITAMDRAIGTLRKHLAATGLRENTLL
                     FYCGDNGTSADASLASPHRGVKGQVYEGGVLVPGIIEWPARIPQPRTTLVRACTVDLL
                     PTLCGLAQQPLPKRPLDGIDLAPLLDGKLTERPGPLRFWAYNTSHLTSQKPRPYIDPV
                     LQQGTTPLAKFAGGKATRDFQNFRQPPLTDADYLGPRGIIDGRFKLVIHEPRMGEAKL
                     ELFDLDADPAEKTDLVPKQPLVASRLQEQLRQWQQSVLSSLSGADYD"
     gene            complement(2290..3102)
                     /locus_tag="VFB80_19855"
     CDS             complement(2290..3102)
                     /locus_tag="VFB80_19855"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN36100.1"
                     /translation="MLLYTRALEAFPGLKQRLFRLVYQYLGRYEYDDWTFMNYGYNTP
                     LRSGEALQLLAEDEPDRNCIQLYECVTAGAQLKGRSLLEIGCGRGGGLSYLHRYRGPR
                     MSRGLDIAPRAVAVCRARHVHPGVGDLRFRVGDALATDFDDGAFDVVVNVESSHCYPS
                     RFDFFLEVRRVLKPGGTFCYADLHEKEAAAGIESALEGAGFAVTQREDLTPGVLRSMA
                     ADDSRKREMILARAPWVLRRAFNSFAGVVGSRTHRSFVVGARQYTRWLLSKV"
     gene            complement(3127..3297)
                     /locus_tag="VFB80_19860"
     CDS             complement(3127..3297)
                     /locus_tag="VFB80_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36101.1"
                     /translation="MQYLAYTIIVLGYLSDYLAVLADGAAATAAQMKAENDQFQQAAL
                     EFSQIDWDRLRQ"
     gene            3524..3766
                     /locus_tag="VFB80_19865"
     CDS             3524..3766
                     /locus_tag="VFB80_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36102.1"
                     /translation="MHYLYGQKSGVRRRLVATFDSQQQLRAYVRWATLAETGPQAGKF
                     EQGSALASYQSWEESPLPLTDEDPQQVVHNPSPSML"
BASE COUNT          768 a         1303 c         1305 g          718 t
ORIGIN      
        1 ctcggtgctg gcctgcggca gggccttctg gtagatcgaa acttccagtt cggcattccg
       61 catcgccgcc cccaggtgct tgcgatgcac ttgcgccagc cgctggcccg tcaccaatcg
      121 catccacaag tacagcggaa acttaggctc gcggccatat tccggcagcc gctgggccag
      181 atccaaatac gcctcctgga gcacgtcgga cgcatcgacc cgcccctgca gtcggcgatc
      241 cagccgcagc ttgaccatcc gcttgagccg gtcgcgatgc tggctgaaga gggccgcgag
      301 ggccgggata tcaccggcca aggcctgttt gaggagcgct tctcccgtgt cgtccgcggt
      361 cattgcaaaa cccatagagg attaaatgca gaccgtcgcc gattttagac cgccaggagc
      421 gagaaaaccg aaaaatggtt gaaaacgcag ccatcaacgg cctgatctta cgcccgtagt
      481 tcgattcgtt agtcgtaatc ggctcctgac aggctgctca gcaccgattg ctgccactgc
      541 cggagctgct cctgcagcct cgaagcgacg agcggctgtt tggggacgag gtcggtcttc
      601 tcagccggat cggcgtccag gtcgaacagc tccagcttcg cttcgcccat tcgcggctcg
      661 tgaatcacca gcttgaagcg gccgtcgatg atgccccgcg ggccgaggta atcggcgtcc
      721 gtgagcggcg gctgccgaaa attctggaaa tcgcgcgtcg ctttgccgcc cgcgaacttg
      781 gccagcggcg tagtgccttg ctgcagaacg ggatcgatgt agggccgcgg cttttggctg
      841 gtcaaatgcg acgtgttgta ggcccagaaa cggagcgggc caggtcgctc cgttagcttc
      901 ccgtcgagca gcggcgcgag gtcgatgccg tccaagggac gctttggcag cggctgctga
      961 gcgaggccgc acagtgtggg gaggagatcg accgtgcagg cccgcaccag cgtcgttcgc
     1021 ggctgcggaa tgcgggccgg ccactcgatg atgcccggca caagcacgcc gccttcatag
     1081 acctgcccct tcacgccgcg gtgcggcgag gcgagcgaag catcggcaga agtgccgttg
     1141 tcgccgcagt agaagagcag cgtgttctcg cgcaggccgg tagcggccag atgcttgcgg
     1201 agcgtgccga tcgcccggtc catcgcggtg atttcggcat aacgttcgcg gagcacctcg
     1261 ccctgcggcc gcgtcacctg gccgcccgtt tcgttcgagg tcaggcggac cttcttctcg
     1321 gcgtactttg ccggcaagtc gtcgtagaga gccagatcgg cgggcaagcc gctgtagggc
     1381 tcgtgcggcg agccgaacca gacgatggtg aagaacggct tgctctcggc gcggacgccg
     1441 tcgatgaagc ggattgcctc gcggacgaga atctcggagc tttcgcccgc gatcacctcg
     1501 ggcgggccgc cgttgcgcga cagggaggga ttgagctcga aaaagttgtc gtgtgagagc
     1561 cactcgtgaa agcccatcgc tccggggcag accggcgagg cggctttcac cgggccgacg
     1621 tgccacttgc caaaatgtcc gcagcggtag ccggccggcc gcaggatctg ggcgatcgtg
     1681 atctcctcgg gccggaagga ccagcccgga gcaaacgtgc cgtaacggtt gggatgccgc
     1741 ccggtcagga agctgccgcg agtcggcgag caattcggat gggcggcgta gaaccgatcc
     1801 atccgcagcc ccgcggccgc catcttgtcg agctcgggcg ttttcacgtg cggatgcccg
     1861 tgatagccgg tctcctccca gccgtgatcg tcgcccatca gcaggacgat gttgggcggt
     1921 gcggctgagg cgcgcgccaa agtcagaacg acgacgaaca agccaagggc aaatcgattc
     1981 atgtcagtcg gtgagctgat cagcgtcatc agccaacgtc tgcgcccaga tcggttccag
     2041 gcgccgacaa cgcagcggcg gtgcctgaat cctaaatcaa accgcacgtc gtacaaggcg
     2101 caggtgcgga actgaaacgt ctcgctggag gccggccggt cgaagtatgt gcgcagattc
     2161 ccgccgtccg atgatgagta gatcctgaac agggcctccc cacccgcgcg cgttctctaa
     2221 tcatggcgcc aacgtctgtg agctcatgcg aggcatttga gccgagggaa gccagagttg
     2281 tgacatgtcc tatactttgc tcagtagcca ccgcgtgtac tgtcgcgcgc cgacgacaaa
     2341 actgcgatga gtccttgaac cgactacgcc ggcaaaagag ttaaacgcgc gccgcaaaac
     2401 ccagggagct cgggccagga tcatctcacg cttgcgagaa tcatcggcgg ccattgaccg
     2461 cagcacgccg ggcgtgagat cctcgcgctg cgtaaccgcg aagccggcgc cctcgagggc
     2521 cgattcaatg cccgctgccg cctctttctc gtgcaggtcg gcgtagcaga acgtcccgcc
     2581 gggtttcagc acgcgacgga cttccaggaa gaagtcgaag cgcgacggat agcagtgcga
     2641 cgactcgacg ttcacgacga cgtcgaacgc gccgtcatcg aaatcggtgg ccagcgcgtc
     2701 gccgactcga aaccgtaagt cgccgacgcc tggatggacg tgccgcgcac ggcaaaccgc
     2761 caccgcccgc ggcgcgatat ccagaccacg gctcatccgc ggcccgcggt agcggtgcaa
     2821 gtacgataag ccgccgcctc ggccgcaccc aatctccagg agcgagcggc ccttgagctg
     2881 ggcgccggcc gtgacgcact cgtacaactg gatgcaattg cgatccggct catcctccgc
     2941 caagagctgc agcgcctcgc cactccgcaa gggagtgttg tagccgtagt tcatgaacgt
     3001 ccagtcgtcg tattcgtagc ggccgaggta ctggtagacc agccggaaga ggcgttgctt
     3061 cagccccgga aacgcttcga gcgctcgcgt gtagagtagc acccgcgtcc tccctgactc
     3121 ggttaattac tgcctcagtc ggtcccagtc gatctgggag aattcgagcg ccgcctgctg
     3181 gaactggtcg ttttccgcct tcatctgggc ggcggtcgcc gccgcgccgt cggcgaggac
     3241 cgccaggtag tccgacaggt agccaagaac gattatcgtg taagcgagat attgcaccta
     3301 gagcgagatc ccgcaattta gggagatcgc ggcaactcgc tataatttgc tagctcattt
     3361 tctcgctagc ggttgtccct gctccggcgc agcagccgat gattcggtga cagttgttgg
     3421 ccggagggcg aactggcggt gatcatcgag gacgtggccg cctcgcaacg acgcagaaga
     3481 gtgcgaagcg ctgtagttga cacctgcggg tggaggacgc gtcatgcatt acctgtacgg
     3541 gcagaagtca ggcgtccggc gacgcctggt tgccacgttc gactcccagc agcagttgcg
     3601 agcttacgtt cgctgggcca ccctggctga aacgggcccg caagccggga agttcgaaca
     3661 gggcagcgcg ctcgccagct accagagctg ggaagagtca ccgctcccgc tgaccgacga
     3721 ggacccacag caagtcgtcc acaatccctc acccagcatg ctttgatcct ggggcgccag
     3781 gagtccgccg cggcctctgc ctcgctgcac ggctccaggt aatgaaccgc cacgatgcgc
     3841 gcatattgcc aagtttttcc cggacggcag ttcagatcgc aactccaatc gccgcaataa
     3901 gttacgtcga aatccgccgg cagtccatta tgcgcattat gcgcgcctgt aggtcccgac
     3961 tgccgggcgg gacctgtgca gtgcggtagg ccgcggccct ggttccaggc tccgactggg
     4021 agcacgctcc gctgaggcac cggccgcgct gcatgcataa tcgcggaatt cctccaactg
     4081 catatttcga cgca
//
LOCUS       DASRJB010000752         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2185619, whole genome shotgun sequence.
ACCESSION   DASRJB010000752 DASRJB010000000
VERSION     DASRJB010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185619"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2831)
                     /locus_tag="VFB80_19870"
     CDS             complement(<1..2831)
                     /locus_tag="VFB80_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HZN36103.1"
                     /translation="MFTPHDSVRASVAEPNTVGPEAMLREACDDLEERIRRGEAARAE
                     EYLARHPALAADAELALDLIYVEYAARQHRDEPKLREELLARFPHWQAQLQRQFQLDE
                     LMDEPSTQCESDDGQTGQLPASEQAGAAARRFRTLGLLAKGGIGQVLRAVDTELNREV
                     AVKELQPAHADNSEVRERFLREAEITGQLEHPGIVPVYGLGSDCQGRPFYAMRLVQGQ
                     SFQEAMEEFHRRAGSPRPLLGVEFQKLLRRFLHVCETVAFAHSRGIIHRDLKPANILL
                     GPYGETLVVDWGLAQATGASEQFATSGQRAEAAAADSLTTPAAHWSSALTQISGELIG
                     TPAFMCPEQAAGAAHDVGPAGDVYSLGATLYMLLTGHPPFVGADVSETLNRVSAGRFA
                     RPREISRAIPRALEAVCLKAMARRPADRYASPRELADDLEHWLADEPVTAAREWLVAR
                     LARWGRRRRSYVVAGSLALVLITVVAVTAAFRIDGERRRADRARGEADRQRSEADRQR
                     IVANRRAAGLAFARGFTLVENEENGVGMLWLSRALQYAPADDAALRRAILTNLSAARQ
                     HLVRRRVAFHHPANIWKLAFSPDGATCLTVDRDGTARVWDADSGALKAAHPLGSIALF
                     AVAIRGDGTPLVAVVEGHSLAVKSLPAAPGDSTAADVVLAHPDNLLLAAFSPDGSQIC
                     TVTGPPSGPQPASIRIWHTADGQQLTQFAGPTAVESVAFQPQGPAVATLSRDGLVRLW
                     NASDGGRLGNPLQVAGTSVMRMDFTPDGSRLLLGGRDGSLTCWNVGSRRRMFSLPTDR
                     RGEFAGLACASDGQTVVASSADGTARAWHMQDQRTLGDLIHLDRHAGILAFRPGTRQI
                     LLAAEPRSAVLWDIPAKTRAAPRLGQGPVQTVAFSPDGSTAATGTRNGIAHLYDAVTG
                     RPLGAAMQHAGTIQHVSFRRDSAVLVTAS"
     gene            2817..3506
                     /locus_tag="VFB80_19875"
     CDS             2817..3506
                     /locus_tag="VFB80_19875"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36104.1"
                     /translation="MRREHCGERSLATRLCLNDTPQQDELQLNADNSAFSQPDDDFVN
                     LLARVRGGDDVAAAELVARYERAVLRSVRSRLSPSMRGALDSMDVMQSVHRSLLTGLK
                     IGRFEFATPQQLVGLAVVMVQRKVARHWRKLRRFPLAGSGAADPATAAPLETIASDES
                     APSEIATANDLLERCLAQLNDLDQQLVRLKLSGFSSTESAALLGREPAFIRMRWSRLR
                     QMLRERGHLED"
     gene            complement(4500..4691)
                     /locus_tag="VFB80_19880"
     CDS             complement(4500..4691)
                     /locus_tag="VFB80_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36105.1"
                     /translation="MPQVLSFLPEGRLASWGGAGGSLVIADADRRLTFAYVMNLMRPD
                     LVGPVAEALVKRLHDIMNS"
BASE COUNT          797 a         1623 c         1556 g          803 t
ORIGIN      
        1 ttgctcgcgg tcacaagcac ggcggagtcc cgccgaaacg acacatgctg aatggtgccc
       61 gcgtgctgca tggccgcgcc gagggggcgt ccggtcacgg cgtcgtacag gtgagcgatt
      121 ccgttccggg tgcccgtggc ggccgtggat ccatccgggc tgaacgcgac cgtctgaacg
      181 ggcccttgac caagccgggg cgccgctcgc gtcttggccg ggatgtccca cagcacggcc
      241 gagcggggct cggcagcgag caggatctgc ctggtccccg gacgaaacgc cagaatcccg
      301 gcgtgccggt ccaggtgaat caaatcgccc agcgtgcgct gatcctgcat gtgccaggcg
      361 cgggccgtcc cgtcggccga tgacgccacc accgtctggc catccgacgc acaggccagg
      421 cccgcaaatt cgccacggcg atcggtcggc agcgagaaca ttcgcctgcg gctgcccaca
      481 ttccagcagg tcagcgagcc atcccgcccg cccagcagga gccggcttcc gtcgggtgta
      541 aagtccatcc gcatcacgct ggttccggcc acttggagcg gatttcccag cctgccgcca
      601 tcgctggcat tccacagccg caccagccca tccctggaga gcgtcgccac cgccggtcct
      661 tgcggttgaa acgcgacgct ctcgaccgcg gttggcccgg caaattgtgt gagctgctgg
      721 ccgtctgcgg tgtgccagat tcggatgctg gccggctgcg gaccgctggg ggggccggtg
      781 acggtgcaga tctgcgagcc gtcggggctg aacgccgcga gtagcagatt gtccggatgg
      841 gccaagacca cgtcggccgc ggtcgagtcg ccgggggccg cgggcaggga cttcacggcc
      901 aggctgtggc cctccaccac cgcgacgagc ggcgtgccat cgccccggat cgccaccgcg
      961 aacagagcga tgctgcccaa agggtgagca gccttcaggg cgccgctgtc cgcgtcccac
     1021 acacgcgccg tgccgtcgcg atcgactgtc aggcaggtcg ccccgtccgg gctgaaggcc
     1081 agcttccaga tgttggcggg gtgatggaag gctacgcggc gacgcaccag gtgctgtcgg
     1141 gcggcgctca agttcgtgag gatcgcgcgc cggagcgccg cgtcatcggc cggcgcgtac
     1201 tgcaaggccc gcgagagcca gagcatgccc acaccgtttt cctcattctc gaccagcgta
     1261 aatccccggg cgaaggcgag cccggcggcg cgacgattcg ccacgatccg ctgccgatcg
     1321 gcctcgctgc gttgccgatc ggcttcgccg cgtgcgcggt ccgcccgccg tcgctcgccg
     1381 tcaatgcgaa acgcagccgt cacggccacg accgtgatca gcacgagtgc cagcgagccg
     1441 gcgacgacgt agctccgccg ccgccgcccc cagcgggcga gccgggcgac gagccactcc
     1501 cgcgccgccg tcaccggctc gtcggccagc caatgttcca gatcatccgc cagctcgcgc
     1561 ggcgacgcgt aacggtccgc gggccgccgc gccatggctt tcaggcagac cgcttccagc
     1621 gcccgcggga tggctcgact gatttcgcgt ggccgcgcaa aacggccggc gctgacgcgg
     1681 ttcaaggttt cgctcacgtc cgcaccgacg aacggcggat ggccggtgag cagcatgtag
     1741 agcgtcgctc cgaggctgta cacgtcgccg gccggcccaa catcgtgggc ggcccccgcc
     1801 gcctgttctg ggcacatgaa cgccggcgtg ccgatcaatt caccgctgat ctgcgtgagc
     1861 gcgctcgacc agtgggccgc aggggtcgtc agcgagtcag ccgcagcagc ctccgcgcgc
     1921 tggccgctgg ttgcgaattg ctcgctggcg cccgtcgcct gagccaggcc ccaatccacg
     1981 acgagcgtct cgccataggg acctagcagg atgttcgccg gcttgaggtc gcggtgaatg
     2041 atcccgcggc tgtgcgcaaa ggccacggtt tcgcaaacgt gcagaaaccg ccgcaggagc
     2101 ttctggaact ccacgcccag caagggccgc gggctgccgg cccgccgatg aaactcctcc
     2161 atcgcctcct gaaaactctg cccttgcacc aggcgcatgg catagaacgg ccgcccctgg
     2221 caatcgcttc ccaacccgta cacgggcacg atgcccgggt gctcgagctg gccggtgatc
     2281 tccgcttccc gcaggaatcg ttcgcggacc tcgctgttgt cggcgtgcgc cggctgcaac
     2341 tccttgacgg cgacttcgcg gttcagttcc gtatcgacgg cccgcagcac ctgcccgatg
     2401 ccgcccttgg cgagcaatcc tagtgtgcga aaccggcgag ctgcggcgcc agcttgctcg
     2461 gacgctggca gctggccggt ctggccgtcg tcgctctcgc actgcgtgga cggctcatcc
     2521 atcagctcat cgagctggaa ttggcgctgc agctgagcct gccaatgggg aaaccgcgcc
     2581 agcagttcct cgcgcagctt cggctcgtcc ctgtgctgcc gcgcggcata ctcgacgtag
     2641 atcaagtcga gcgccagttc ggcgtcggcg gccagcgccg ggtgccgggc gagatactcc
     2701 tccgctcggg ccgcttctcc ccgccgaatc cgctcctcca ggtcgtcgca agcctcccgc
     2761 aacatcgcct ccgggcccac cgtgttcggt tcggcgacgg aagcacggac gctatcgtgc
     2821 ggcgtgaaca ttgcggcgag cgaagcctcg cgactagact gtgcttgaac gatactcctc
     2881 agcaggacga gttgcaattg aatgccgaca actccgcctt ctcacagccg gacgatgact
     2941 tcgtgaatct gctcgcccgc gtgcgcggcg gcgacgacgt tgccgccgcc gagctggtag
     3001 cgcggtacga gcgggcagtg ctgcgttcgg ttcgcagccg gctgagcccg agcatgcgcg
     3061 gtgcgctgga ttccatggat gtaatgcagt cggtgcatcg cagcctgctg acggggctga
     3121 agatcggacg ctttgagttc gctaccccgc aacagctggt cggattagcc gtggtcatgg
     3181 tccagcgcaa agtggcccgg cactggcgca aactaaggcg ctttccgctg gctggcagcg
     3241 gggccgcgga ccccgccaca gccgctccat tggagacgat tgcgagcgac gagtcggctc
     3301 cctccgaaat cgcgactgcc aacgacctcc tcgaacgctg cctcgcacag ctaaatgacc
     3361 tcgaccagca attggtcagg ctcaagctca gtgggttcag ttccaccgag tccgccgcac
     3421 tcttgggccg cgagccggcg ttcatccgca tgcgctggtc ccgcttgcgg cagatgcttc
     3481 gcgaacgtgg tcacttggaa gactagcgcg atgactgcag tgtcaccgcg gcgcttcggt
     3541 ccgacacgcg aggagccccg gccggcgcga cccgccgcac ctttcgtcga tgtagcgctg
     3601 tacgatcgtc gccagctgtg gaaacagaac ttgcggtggt gcctcgcagc gcccgctgtc
     3661 gcagtagtct aaggctgcgg tcgaaggcga agcagtttcc aaagcagcag gcgtccgcag
     3721 aacctggcac caatcggcga tcggctcgtg cgacgcgaag gtcagcctgg cgaaatggga
     3781 atcaatgggc ggtaagggcc taatggagtc cgttccaagc gcaagcggag actgcacttc
     3841 cggcgaggca gcaaagtcag gcgaagcaga atccgaagca gttttgcccg aaacgtcctc
     3901 agtcgatcct gatttggcag tcgtcgtcgc ggcttggcgg acactgcccg atgaaatccg
     3961 agcccggatc gtggcgctgg tttcggggcg cactggcggg taaagggtgc cgaaggtgtc
     4021 agtgtacgcc aacacctagc agagtgcgcc agagtgcatc agtggcgtag actggcgatc
     4081 gtggcgcaga ctgtcagcat cttcggacgg gctgagcatg atcgcccgcc gggctcgcgg
     4141 aatttgcagt tgctctctta cacgcgccac ctgggatgcc cgcggccatt tatgacagac
     4201 tatagcgcgc tgtctcctgg atgcctacaa gctccgctgc agcgtttggc cgtccatcga
     4261 ctaaaagggg ccgaacttgc cttgacgaag cacgtagcgg gcttctacgg acgtcgtgag
     4321 gctcgataaa ggttcgctca ctcgaaagcc ggtcgccgca cactggtgag tcgtgcaacg
     4381 tctagcatct gattgcgaac cgatgctctt tcatgctctc ccatgctttt gtcgctttgc
     4441 tgttgggccc ccgatccaac gctgcacggg ccgccggcgc cctgcgagag tttccgttct
     4501 cagctgttca tgatgtcgtg cagacgcttg accagcgcct ccgcgacggg cccgaccagg
     4561 tccggcctca tcaagttcat cacgtaggcg aaggtcagtc ggcgatccgc gtcggcgatg
     4621 accagcgagc cgccagcgcc gccccaggag gccagccggc cttccgggag gaaggagagc
     4681 acctgcggca tgggaagcag gtcaggtgca ctgactcgaa atcactcgac acttcgcgaa
     4741 ccaattcgac tcgttgcgag ttattaaata attattcaa
//
LOCUS       DASRJB010000753         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_783856, whole genome shotgun sequence.
ACCESSION   DASRJB010000753 DASRJB010000000
VERSION     DASRJB010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783856"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFB80_19885"
     CDS             complement(<1..975)
                     /locus_tag="VFB80_19885"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36106.1"
                     /translation="MLSIYDPLPVRDCQGLRRREFLRAGALGLFGTTLAGLAGRAAYA
                     ADGLRDYATGKSVVLLFLGGGPSHIELFDPKLTAPAEIRSITGEVPTVHPGITFGGTF
                     PQLAKLAKKFSIVRSFQSLNSGHEYHAVTTANNPTKAVAGALYTRVAGMNHPRTGAPR
                     HTLVLPEAVAPPGFKIGTNFETGHLPGLTQTGSLGPNYQAFNPSGGGQLRENMQLAIP
                     AERLTDRRAVLSSLDRLRRQVDASGKIEGLDKLQQQAYEVITGGVADAFDLSKEDPRT
                     VARYDTSHCFQSEDAQKFGDMRRSTNLLGRQMLLARRLIESGCGFVTVS"
     gene            1604..2398
                     /locus_tag="VFB80_19890"
     CDS             1604..2398
                     /locus_tag="VFB80_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36107.1"
                     /translation="MNRTSLLGSLVLGFAVCTQGFSFELLDRMLGYSGCGCDTSCCSS
                     SCCKSRCHGHHNRCGCGCDNGCGKGVGCGVAAPGCGVAAPTCGVAAAPTCAAAAPACG
                     VDGKAACGCDNGCDRCGRCGHHRNRCGCGCDKGCGHHNGCGCNKGCNSCGCDKGCGHH
                     NGCGCDKGCNSCCKRGCFLDRMFSGHGCGCGCDNGCGKGVGCGVAAPTCAAAAPAPAC
                     GCDGGPVKVGGPAADAVPMPPAPVVDPSAFIPTQRRVVHATTSLVR"
BASE COUNT          391 a          852 c          887 g          466 t
ORIGIN      
        1 ggaaacggtg acgaagccgc agcccgactc gatcagtcgg cgggcgagga gcatttgccg
       61 gccgagcagg ttggtcgagc ggcgcatgtc gccgaatttc tgggcgtctt cgctctggaa
      121 gcagtggctg gtgtcgtagc gggccacggt tcgcgggtct tcctttgaaa ggtcgaaggc
      181 gtcggccacg ccgccggtga tcacttcgta ggcctgctgc tggagcttgt cgagcccctc
      241 gatcttgccg ctggcatcga cttgccgccg caggcggtcg aggctcgaca gcacggcccg
      301 ccggtcggtc agccgttcgg cggggatggc gagctgcatg ttctcgcgga gctggccgcc
      361 gccgctggga ttgaaggcct gatagttcgg ccccagcgag ccggtctgcg tcaggcccgg
      421 caggtgcccg gtctcgaaat tcgtgccgat cttgaaaccc ggcggagcga cggcttcggg
      481 caggacgagc gtatgccgcg gcgcgccggt ccgcggatgg ttcatcccgg caacgcgcgt
      541 ataaagcgcc ccggccaccg ccttggtcgg gttattggcc gtggtgacgg cgtgatactc
      601 gtggcccgaa ttcagcgact gaaacgagcg gacgatcgag aacttcttcg ccagttttgc
      661 cagctgcgga aaagtgccgc cgaaggtgat gcccggatgc accgttggca cttcgcccgt
      721 gatgctgcga atctcggccg gggcggtcag cttgggatcg aacagctcga tgtgcgacgg
      781 cccgccgccc aggaagagca atacgacgga cttgcccgtg gcatagtcgc ggaggccgtc
      841 ggccgcatag gccgctcgtc cggccaggcc cgccagcgtc gtcccgaaga ggccgagcgc
      901 cccggcccgc aggaactcgc gccgccgcag gccctggcaa tcgcgtaccg gcagcggatc
      961 gtaaatcgag agcatgggcg ggccctcagg gatcgaccat cgttgattgt atcgaccggc
     1021 gcggcgggca tgtcaaggtt tgacgagcaa tgggaccaat gagaccgatg ggacgtaagg
     1081 ggctggagtg tgaaaccctg cggcctggca gtgactttgc gtgggtccaa cacgggtctg
     1141 ctgccgcatc accggcacga gcgccacagc ttccggcggc ggcaatcttt gcgcgactgt
     1201 cgtcctaaac cgatgcctga aaattatttg cgtcagtttc aagccctctg cgcgggcgct
     1261 ccgatacggt agttgtcgct ggactctcgc ggccgcgcca tgatggcctg gccggcaaga
     1321 gtcaggtgaa ccggagcgga caaattcggg ctgagcaatc ggctcgaacc ggttctggtg
     1381 ctcgccaccg ccttccgtgt gggacaacga ccccgccttc ccgcacacct ggcctacccg
     1441 tactcgcaca ccccgcaaga ttccgacggc aatgggactg ccggcgggat cggtccaacg
     1501 tggcctcgtc ggctgcgact cgcgcgccac cgacgaattc acgtgtccgg ccggaccggg
     1561 acatgccagt tcgatggttc catcgtttag gagacgtcgc tcaatgaatc ggacctcgct
     1621 tctcggatcg ttggtactcg gcttcgccgt ttgtacccaa ggtttcagct tcgagctgct
     1681 cgaccgcatg ctcggctaca gcggatgcgg ctgcgacacg agctgctgct ccagctcgtg
     1741 ctgcaagtcg cgctgccacg gccaccacaa ccgctgcggt tgcggctgtg acaatggttg
     1801 cggcaagggc gtcggctgcg gcgtggccgc cccgggctgc ggcgtggccg ctccgacctg
     1861 cggcgttgct gccgctccca cctgtgcggc tgccgccccc gcttgcggcg ttgacggcaa
     1921 ggccgcttgc ggctgcgaca acggctgcga tcgctgcggc cgctgcggcc atcaccgcaa
     1981 tcgctgcggt tgcggctgcg acaagggctg cggccaccac aacggctgcg gctgcaacaa
     2041 gggttgcaac agctgcggtt gcgataaggg ttgcggccac cacaacggtt gcggctgcga
     2101 caagggctgc aacagctgct gcaagcgcgg ctgcttcctg gaccggatgt tctccggcca
     2161 tggctgcggc tgcggctgcg acaacggctg cggcaagggc gtgggctgcg gcgtcgctgc
     2221 cccgacctgt gcggctgccg ctccggcccc cgcttgcggc tgcgacggcg gcccggtgaa
     2281 ggttggcggt cccgctgctg acgcggtccc gatgccgccg gctccggtgg tcgatccctc
     2341 ggccttcatt ccgactcaac gtcgggtcgt tcacgccacc accagcctgg tccgctagga
     2401 ctggtttggc ttaaggctac gacaatcaag cggcctggcc gggaccttcc cgatcaggcc
     2461 gctgttcgtt tcttgccgcg ccgtgcgcta cttcagcggc acggcgtgat cgtcgatata
     2521 cgtctccacc gccgagcgcc gcggcggccg gctgtcgatg tagtggaacg cctcggcgta
     2581 ctccacgccg tattgt
//
LOCUS       DASRJB010000754        16680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_506516, whole genome shotgun sequence.
ACCESSION   DASRJB010000754 DASRJB010000000
VERSION     DASRJB010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506516"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFB80_19895"
     CDS             complement(<1..656)
                     /locus_tag="VFB80_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HZN36108.1"
                     /translation="MAEISRVALFGKLNSLGYRAIEAATVFCKMRGNPYVELVHWFHQ
                     ILQLQDSDLHKIIKHFQLDAARLVKDLTENLDKLPRGASSITDLSSHLEEAVERGWVY
                     GSLVFKDSQVRTGYLVIGILKTRGLTNALLGISKEFAKVNADALLDKFGEACGGSPED
                     ALRANDGSNLGGATPGEASGAMAPAQMGKQEALKQFTIDWTDRAKKGELDPIVGRDDE
                     "
     gene            complement(722..1747)
                     /gene="tssG"
                     /locus_tag="VFB80_19900"
     CDS             complement(722..1747)
                     /gene="tssG"
                     /locus_tag="VFB80_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZN36109.1"
                     /translation="MAGTDRAAADRLKLFAELSERPYDFSFYQALRKLEAAFPERPRI
                     GRSLRPTDDPVRFCQQPSLAFAPASVAAFQSGQGDLPTRLTVWFGGLLGANGPLPLHL
                     TEYARDRTRNGNDWTFVRFLDLFHHRMLSLFYRIWADAQPTVQFDRPASDRFAAYVGS
                     LAGVGMPSLHNRDAMPDLAKLSFAGRFVCQSRHAEGLQAILGHFFQLPMRIAEFVGQW
                     LPLPPDCRCELREAGGMAQLGFSATIGDRVWDCQTKFRVLAGPMGFADYQRLLPGGES
                     LERLIAVVKNYTGLELDWDLHLLLKRREIPQTQLGNLGQLGWTTWLVSQPPKADADDL
                     ILTPQAA"
     gene            complement(1711..3591)
                     /gene="tssF"
                     /locus_tag="VFB80_19905"
     CDS             complement(1711..3591)
                     /gene="tssF"
                     /locus_tag="VFB80_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HZN36110.1"
                     /translation="MDPRLLKYYNRELSHLREMGGEFAAEFPKIAGRLSLDSIECADP
                     YVERLLEGFAFLAAKVQLRLDSEFPRFTHHLFEMVYPHCVHPTPSMAVVQFKPDLKEG
                     SLAEGFVVPRDTPLKGLLGKGEATACEYRTAHKVTLYPLELIEAQYVSRDSAGMELPD
                     VPRLKAAIRLRLRSTAGLPLNKLKLDKLPLFIHGAGELPVRVYEQILAGCVGIAARPV
                     QRPAPWQVVLPPTSVRRLGYDDHEALLPYGPRSFQGYRLLAEYFACPQRYLFVELSGL
                     SAAIQKSSEQTIDVILLLDRYEPQLEAYVGAANFGLFCSPAINLFPKTADRIHLTDQM
                     HEHHVVPDRTRPMDFEVYSVTSVVGYGTNLDEEQPFLPFYSATDATAAAGAQRAYYTV
                     LRTPRVFSTNQKKFGPRSSYVGSEVYLSLVDSSESPYSHDLRQLAVSALCTNRDLPLH
                     MAVGKSTTDLTLESGAPVDSIRCLAGPTRPRASPVFTDGDFVWRLVSHLTLNYLSVVS
                     DKDGRGAAALRDLLALYGDVNDAPTRKQIEGVRNVASQTITRRIPTAGPITFGRGLEI
                     AVTCDEAAFEGTGMFLLGSVLEQFFARYASLNSFTETVLHSAGRGEVMRWPARIGRRQ
                     IA"
     gene            complement(3703..4206)
                     /gene="tssE"
                     /locus_tag="VFB80_19910"
     CDS             complement(3703..4206)
                     /gene="tssE"
                     /locus_tag="VFB80_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HZN36111.1"
                     /translation="MAELTPSERLQPSLLDRLTDEEPAKNQESRNRRVLSVQRLRESV
                     LRDVAWLLNSASLPEDDLEANYPHVAKSVVNYGLPDLAGLTASGIDVAELEQIVKQAI
                     WDFEPRIMRHTLKVRARTDSGDMSHNALVFEIEGELWAQPIPLELLLKTEIDLETGDI
                     AVTEVTR"
     gene            complement(4199..5086)
                     /locus_tag="VFB80_19915"
     CDS             complement(4199..5086)
                     /locus_tag="VFB80_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HZN36112.1"
                     /translation="MSADELLRDGKLDEALAALTQQVRAKPADAKLRTFLFQLLCVQG
                     QWERALNQLNVAGEMDAGTLAMVNTYREALRCELLRAEIFAGKRSPLVFGQPAEWVAQ
                     LIQALSLTAAGKHSAAAELRERAFEAAPATSGVIATARPGANDSRVPPVEATFQWLAD
                     ADSRLGPVIEAVFSGKYYWVPLVNVRRIDVEPPSDLRDVVWMPAHFVWPNGGDTVALI
                     PTRYAGSEANGDPLIRLARKSDWNEAAPGVFLGQGQRMLATDAGEYPLMDVRQIRFDV
                     PLTDGPLAAAGEGAAEEHG"
     gene            complement(5131..5640)
                     /locus_tag="VFB80_19920"
     CDS             complement(5131..5640)
                     /locus_tag="VFB80_19920"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HZN36113.1"
                     /translation="MILLHLDKCPGDSKIADHTNWIACTSVSWNIERTFSESAKAGTL
                     DVNVGVADIPPISVNKTLDVASTYLMSAGIAGGSLGKEAKLHFLFADQEGKQHLYLEF
                     ILANPIIASWSISGDEDERPTEECSIWYWAIWMQYHTWDGSTNKAAGSKGWDRTKNKP
                     WNGKDPVGA"
     gene            complement(5818..7314)
                     /gene="tssC"
                     /locus_tag="VFB80_19925"
     CDS             complement(5818..7314)
                     /gene="tssC"
                     /locus_tag="VFB80_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HZN36114.1"
                     /translation="MSTDMSQAQAAPQAQAAELNDFSALLSKEFKPKSDRAKEAVEEA
                     VKTLAEQALAGTAVISDDAIKTIQAIIAALDKKLTEQVNQILHHSDFQQLEGAWRGLS
                     YLVNNTETDEMLKVRVFNISKKDLGKTLKKFKGTAWDQSPLFKKMYEEEYGQFGGEPF
                     GCLIGDYHFDHSPPDVEILGEIAKISAAAHAPFITGAAPTLMQMDSWQELGDRRDLTK
                     IFGTPEYAPWRSLRESDDARYLGLAMPRFLARLPYGSKTDPVSEFDFEEACTGADHTK
                     YTWCNAAYAMATNINRSFKMYGWCSRIRGIESGGAVEGLPCHTFPTDDGGVEMKCPTE
                     IAIADRREAELAKNGFMPLVHKKNSDFAAFIGAQSLQKPAEYDDPDATANANLSARLP
                     YLFATCRFAHYLKCIVRDKIGSFKERDQMQSWLNSWIANYIEPNPAMATEEDKARKPL
                     AAAEVTVEEIEGNPGYYTSKIFLRPHYQLEGLTVSLRLVSKLPSGKGG"
     gene            complement(7354..7863)
                     /gene="tssB"
                     /locus_tag="VFB80_19930"
     CDS             complement(7354..7863)
                     /gene="tssB"
                     /locus_tag="VFB80_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HZN36115.1"
                     /translation="MAKASSQKFIARNRAPRVQIEYDVEVYGAEKKIQLPFVMGVMAD
                     LSGKPAEALPAVGDRKFLDFDVDNFDERLKSMKPRVAFQVPNTLTGEGNLNVDITFES
                     MDDFSPAAVAKKVGALSKLLETRTQLANLLTYMDGKSGAEDLIGKALKDPTLLQALAS
                     APKAEEKAE"
     gene            complement(8017..9090)
                     /gene="tssA"
                     /locus_tag="VFB80_19935"
     CDS             complement(8017..9090)
                     /gene="tssA"
                     /locus_tag="VFB80_19935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HZN36116.1"
                     /translation="MLSPVSAEAPCGENLEYDVDYVMMEQAAVGKPEQQFGETVIPAE
                     DPDWRQLQSQAQALLPRSKDLRIAVCLTRAAVRTSGLAGLADGLEVLAGFIEKYWDGV
                     YPQLDPDDNDPTQRVNSLASLTSAQETLKGVREATLVASRGAGKFSYRDVLIASGEMP
                     PLAGTPKVEQSSIDGAFADSDPAELKATGKVLERALGMARQLESNFNDRVGAGSGPEL
                     KPLMDVIAAMSKLVNQKLAQRGLLDDAAPAAPAAADKSDGNGQAAGAASNGRKAGAAA
                     PLTGDIASREDVIRALDKICDYYKRYEPSSPVPLFLNRAKRLASKSFLEILRDLTPDA
                     VNQALAIGGISDGEASSAAPNDI"
     gene            complement(9351..10613)
                     /locus_tag="VFB80_19940"
     CDS             complement(9351..10613)
                     /locus_tag="VFB80_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36117.1"
                     /translation="MTPPSKRSTAIRRPLLAVLLLVAARVAAAQVVVNLPNPQVDASG
                     RLVVQPNVSGTTYVPPPVQPPAVAAIGAVAPVSATIQDESLPPPPPADGGWPGGPLDA
                     KPMLPLLRGEPSDIWLSGEPLVPWDQIEHGWDTWTEHVRSPTGEAGLGRERVAYAPFE
                     IDITQPFGNFLWRTQAVYDLTKPDRAEAFWARPGRGPLLPESSVDYQEFRLRLETGSN
                     AFSLATDVPIRLLNPEQNDNTGGIGDIELVQKTRLIDGNRWQMTQLLRTVFNSGNAKK
                     GLGTGHVSMEPGMLWRYRYSDITFLHSELKMTFPVPGTPGYAGPLLKWGIGVSHVWYE
                     TDTTAYIPTLEFTNIWVLDGQFIPFPGGLPVDVRGDGIFNLAPGLRVVHDTGGDVGVV
                     EFGYSGVFAVGSDGWYDALLRFDLRFVF"
     gene            complement(10720..12198)
                     /locus_tag="VFB80_19945"
     CDS             complement(10720..12198)
                     /locus_tag="VFB80_19945"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN36118.1"
                     /translation="MGEKVVEDPYAITDAAIQEPPQSLWKALAKIGPGIVLAGSIVGS
                     GELILTTSLGADWGFVFLWLVLFSCVIKVFVQIELGRYAISSAQPTLTALNSLPGPRL
                     GASWLVWWWFCMLLASVFQLGAMVGSVGQALNVAFPQVSPAIAGALQSMSAPLAEAIR
                     DRPEHPWSVLTALAAILLLLSGGYKRLEWVTTALVAGVTLITVGCVLLLPSTGYPIRP
                     ADIKEGFNVLAIAALPPAAIAAAFSAFGITGVGASELYAYPYWCLEKGYARFTGPRSD
                     DPKWVERAKGWLRVMILDAWVSMVVFTVATVAFYVLGATVLHRRDLHPKGAQMVVALA
                     EMYVPTFGAWTKILFMIGAWAVLFKTLYVASAGHARLSTDFLGLLGAVRFDDPQKRPR
                     WIRRFCVFFPTLALILYLLFREPKMMVTVGGYAQAVTLPVISGAALYLRYFRTDKRLA
                     PSLASDLLLWTAFVLITAVAGYAVYNDVFPTVGGWLGLVKKG"
     gene            complement(12281..12661)
                     /locus_tag="VFB80_19950"
     CDS             complement(12281..12661)
                     /locus_tag="VFB80_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36119.1"
                     /translation="MRRCWLALAGLIIVTAASPAAAQELLQSRADELRGDIAKIRAAM
                     GVLTRQLEALEKELAAVEPRASVPTRPVPYVPAYRHPLPPGLRFPVDVERAMMGGAVS
                     EPVRPRLESRLNSGETRLVPSPRK"
     gene            complement(12745..13227)
                     /locus_tag="VFB80_19955"
     CDS             complement(12745..13227)
                     /locus_tag="VFB80_19955"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HZN36120.1"
                     /translation="MQSLADLASEVDRVCASAAAGRVRPLGNWTAAQVLQHLALFIAG
                     SLDGFAFQYPLRLRCQAWLLGLISWPLLMRLAFRPGFRNPPAAASVEPDPAITLEHAA
                     ANLRRQLQRLASGERMTHRSPTGERLSHDQWLECHLRHAELHLSFLLLEAEPAASPRS
                     "
     gene            13363..14493
                     /locus_tag="VFB80_19960"
     CDS             13363..14493
                     /locus_tag="VFB80_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36121.1"
                     /translation="MLRCCPGALVLCLVAVSLPAAEGDLADALARIRAVQPRGVGHRE
                     AAAAARVVAAARASALPQILAAMDGAGPIAENWLRGAAEAVAQRGGKQLPVAGLEAFL
                     ADTKHSPRGRRLAYELIAAVDPTAESRLIPTLLDDPSLELRRDAVAQVLAAAGKAEGK
                     EAAPLYAKAFAHSRDLDQIKLAFDKLKELGTPPDIARHMGYVRQWRLIGPFDNVEDAG
                     WDKAYPPETAIDLAAELSGQKGRVKWISHASTDDYGLVELTKALDKHKGAVTYAYAEF
                     VAEREQPCELRLSSPNANKVWLNGELLSANHVYHANDPLDQYIARGKLRKGPNAILVK
                     VCQNEQEEDWAQRWEFKLRVCDTIGTAILGKGSGFGVQGSGG"
     gene            14529..15728
                     /locus_tag="VFB80_19965"
     CDS             14529..15728
                     /locus_tag="VFB80_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36122.1"
                     /translation="MPKTPLLFVLLFAPTLSAADWLQFRGEGNRGVAENERLPTKFGP
                     DEKVAWKAALPGKAACGPIVIGDRVVVTAASGPVVQDRLHVLCLDAKTGQQLWQRQFW
                     ATGRPHHHPMSSNAAPTPASDGKRIFAFYSSNDVVCLDLDGNLLWYRGLGHDYPNTGN
                     DIGMSASPLVVEGTVVVQVECQGDSFAAGLDTATGENRWRINRPHAPNWVSPAALRRA
                     DGTQVVLLQAADGLTAHEPKTGKQLWKYEADCSSIPSPTTAGDRIFLPAAGMTVLEAP
                     ANSSAPSLLWDSNKLAPGNASPVIDGERLYVLSRAGVLAAADAKTGDVKWQTRLKGTF
                     WATPVVAGGHAYCANQDGECLVVQLGEKGEIVHTAAFGEPFLGTPAIAGGALYLRGEK
                     HLWKIGN"
     gene            15761..>16680
                     /locus_tag="VFB80_19970"
     CDS             15761..>16680
                     /locus_tag="VFB80_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36123.1"
                     /translation="MANEIQLKLLNEHGRKEIFLSLARVPGSSRVLCGASDGNVYDLD
                     ALAEKPEFNALGGHASFVTGVAIAGELLVSGGYDGKLLWRKLGGGEIVHTVESAHSKW
                     IRKLAASPDGKLVASVADDMVCRVWEAESAKKLHELRGHDEQTPNHFPSMLYTCAFSA
                     DGRQLATADKVGRICLWDAASGNKLRQLEAPGFYTWDPKQRIHSIGGTRAVAFSPDGK
                     QLVAGGIGTIGNIDHLDGPARVELFEAESGQSQHVFSGDAKGLVEQVLFHPEGKWLLA
                     VGGDNGGLIQAYDLAEKKVTRSDKAPMHIHA"
BASE COUNT         2909 a         5390 c         5530 g         2851 t
ORIGIN      
        1 atttcgtcgt cgcggccgac gatcggatcg agctcgcctt tcttggcccg atcggtccag
       61 tcgatcgtga actgcttgag ggcctcctgc ttgcccatct gggccggcgc catcgcgccg
      121 ctggcttcgc ccggtgtcgc cccgcccagg ttgctgccgt cattcgcccg cagcgcgtcc
      181 tccggcgagc cgccgcaagc ttcgccgaac ttgtccagca gcgcgtcggc gttgaccttg
      241 gcgaactcct tgctgatccc gagcagcgcg ttggtcaggc cgcgggtctt gaggatgccg
      301 atcaccaggt aaccggtgcg gacttgcgag tctttgaaca cgagcgagcc gtagacccag
      361 ccccgctcga ccgcctcttc caggtgcgac gagaggtcgg tgatcgacga cgccccccgc
      421 ggcagcttgt cgaggttctc ggtcaaatcc ttgaccagcc gggccgcgtc gagctgaaag
      481 tgcttgatga tcttgtgcag gtcgctgtcc tggagctgga ggatctgatg gaaccagtgc
      541 accagctcga cgtaggggtt gccccgcatc ttgcaaaaca cggtcgccgc ctcaatcgcc
      601 cggtaaccca ggctgttgag cttgccgaac aaggcgacgc ggctaatttc cgccatttca
      661 ggatctccgg gaatggaacg cggtttagcg ctccgatttc agatttggga tttcagattt
      721 gctaagcggc ttgcggcgtc agaatcaaat cgtccgcgtc ggctttcggc ggctggctga
      781 cgagccaggt agtccagccg agctggccga ggttgccgag ttgagtttga gggatttcgc
      841 gccgcttgag gaggaggtgc aggtcccagt cgagctccag gccggtgtaa ttcttgacca
      901 cagcgatcag ccgctcgagg ctttcgccgc cgggcaagag ccgctgatag tcggcaaaac
      961 ccatggggcc ggccaggacg cggaatttcg tctggcagtc ccagacgcgg tcgccgatgg
     1021 tggcggagaa gccaagctgg gccatgcctc cagcctcgcg cagctcgcag cggcagtcag
     1081 gcggcagcgg cagccactgg ccgacgaatt cggcgatccg catcggcagc tggaaaaaat
     1141 gccccaggat cgcctgcagg ccttcggcgt gccgcgactg gcagacgaag cgcccggcga
     1201 acgagagctt ggccaggtcg ggcattgcgt cgcggttgtg caggctcggc atgccgacgc
     1261 cggccagcga gccgacgtag gccgcgaacc ggtcactggc aggccggtcg aactgcaccg
     1321 tgggctgggc atcggcccag atgcggtaga acagcgacag cattcggtga tggaacaggt
     1381 cgagaaaccg cacgaacgtc caatcgttgc cgttgcgcgt gcggtcgcgg gcgtattccg
     1441 tcaggtgcag aggcagcggg ccgtttgcac ccaggaggcc gccgaaccag accgtcagcc
     1501 gggtcggcag atcgccctgg ccgctttgga acgccgcgac cgaggcgggg gcgaaggcta
     1561 gcgagggctg ctggcaaaag cgcaccggat cgtcggtcgg gcggagcgag cggccgatgc
     1621 ggggccgctc ggggaacgcg gcctccagct ttcgcagggc ctggtagaaa ctgaagtcgt
     1681 aggggcgctc gctcagctcg gcgaagagct tcaggcgatc tgccgccgcc cgatccgtgc
     1741 cggccatcgc attacctcgc cgcgtccggc gctgtggagt acggtttccg tgaatgaatt
     1801 gagcgacgca tagcgggcga agaactgctc cagcaccgag ccgagcagga acatgcccgt
     1861 gccttcgaag gccgcctcgt cgcaggtgac ggcgatctcc aggccgcggc cgaacgtgat
     1921 cggcccggcg gtcgggatcc gccgcgtgat cgtctgcgag gcgacgttgc ggacgccttc
     1981 gatctgcttg cgggtcgggg cgtcgttgac gtcgccgtac agggcgagca ggtcgcggag
     2041 cgccgccgcg ccgcggccat ccttgtcgct gacgaccgac aggtagttga gcgtcaggtg
     2101 gctgaccagc cgccagacga agtcgccgtc cgtgaagacc gggctggccc gcgggcgggt
     2161 cggtccggcc aggcagcgga tcgagtcgac cggagcgccg ctttccagcg tcaggtcggt
     2221 cgtcgatttg ccgaccgcca tgtgcagcgg caagtcgcgg ttggtgcaga gcgcgctcac
     2281 cgccagctgc cgcaggtcgt gcgaataggg cgattcggac gaatcgacaa gcgacagata
     2341 gacctcgctg ccgacgtagc tcgaccgcgg gccgaatttc ttctggttcg tcgagaagac
     2401 ccgcggcgtc cgcagcaccg tgtaatacgc ccgctgtgct ccggccgccg cggtcgcgtc
     2461 ggtggccgag tagaacggca aaaacggctg ctcctcgtcg aggttcgtgc cgtagcccac
     2521 gacgctggtc accgaataga cctcgaaatc catcggccgc gtgcggtcgg gcacgacgtg
     2581 gtgctcgtgc atctggtcgg tcaggtggat gcggtcggcc gtcttgggaa agaggttgat
     2641 cgccggcgag cagaacaggc cgaagttggc cgcgccgacg taggcttcca gctgcggctc
     2701 gtagcgatcc aaaagcagga ttacgtcaat cgtctgttcg ctcgacttct ggatcgcggc
     2761 ggacaggccc gacagctcga cgaacaggta ccgctgcggg caggcgaagt attcggccaa
     2821 gaggcgatag ccctggaacg accgcggccc ataagggagc agcgcctcgt ggtcgtcgta
     2881 gcccaggcgg cggacgctcg tcggcggcag gacgacctgc cacggcgctg gccgctgcac
     2941 cggccgcgcg gcgatgccga cgcagccggc caggatttgc tcatagaccc gcaccggaag
     3001 ctcgcccgcg ccgtggatga acagcggcaa cttgtcgagc ttgagcttgt tcaggggcaa
     3061 accggccgtc gagcggaggc ggaggcggat cgccgccttc agccgcggca cgtcgggcaa
     3121 ttccattccg gccgaatcgc ggctgacgta ctgggcctcg atcagctcta gcggatagag
     3181 cgtcactttg tgggcggtgc gatactcgca ggcggtcgct tctcctttgc ccagtagccc
     3241 tttcagcggg gtgtcccgcg gcacgacgaa gccttcggcc agcgagcctt ccttgaggtc
     3301 gggcttgaac tgcaccacgg ccatcgacgg cgtcggatgg acgcagtgcg gatagaccat
     3361 ctcgaacagg tggtgcgtga accgcgggaa ttccgagtcc agcctcagct gcaccttcgc
     3421 cgccaggaac gcaaagccct ccagcagccg ctcgacgtac gggtcggcgc attcgatgct
     3481 gtcgagcgag agtcggccgg cgattttggg gaattcggcg gcgaactcgc cccccatctc
     3541 ccgcaagtgc gagagctcgc ggttgtagta cttcagtaag cgcgggtcca tacgcttggc
     3601 gagcggcaaa aatcaaagaa ccaaagaacc aagataccaa aggaaactga gtgctttgtc
     3661 aattcctccc tttggttctc tggttcttcg gttcttggga tttcagcgcg tcacttccgt
     3721 gaccgcgatg tcgcccgttt cgaggtcgat ttcggttttg aggagcaatt ccagcgggat
     3781 cggctgagcc cacagttcgc cctcgatctc gaagaccagg gcgttgtggc tcatgtcgcc
     3841 gctgtcggtg cgagcccgga ccttgagcgt gtgccgcatg atccggggct cgaaatccca
     3901 gatggcctgc ttgacgatct gctccagctc ggccacgtcg atgccgctgg cggtcaggcc
     3961 agccaggtcg ggcaggccat agttcacgac cgacttggcg acgtggggat agttcgcttc
     4021 caggtcgtcc tcgggcaggc tggcgctgtt caaaagccag gccacgtcgc ggagcacgct
     4081 ttcccgcagc cgctggaccg agagcacgcg gcggttgcgg gactcctggt ttttggccgg
     4141 ctcttcgtcg gtcagccggt cgagcagcga cggctgcagg cgttcggaag gggtcagttc
     4201 agccatgctc ttcggcggct ccttcgccgg cggcagcgag gggcccgtcg gtcagaggca
     4261 cgtcgaaccg gatctgccgg acatccatga gcggatactc gccggcgtcg gtggccagca
     4321 tccgctggcc ctgtcccaga aagacgcccg gcgcggcttc gttccaatcc gacttgcggg
     4381 ccaggcgaat cagcggatcg ccattggcct cgctgccggc gtagcgcgtc ggaatgaggg
     4441 cgaccgtgtc gccgccgttg ggccagacga agtgggccgg catccagacc acgtcgcgaa
     4501 ggtcgctggg cggctcgacg tcgatccgcc gcacgttgac gagcggcacc cagtagtact
     4561 tgccgctgaa gaccgcctcg atcaccggtc ccaaccgcga atcggcgtcg gccagccact
     4621 ggaaagtcgc ttcaacgggc ggaacgcgcg agtcattcgc gccggggcgg gcggtggcga
     4681 tgacgccgct tgtagcgggc gcggcttcaa acgcccgctc gcgcagctcg gccgccgcgg
     4741 aatgcttgcc ggcggcggtg agcgacaggg cctgaatgag ctgggccacc cactcggccg
     4801 gctgtccgaa gacgagcggc gagcgcttcc cggcgaagat ttcggcccgc aggagctcgc
     4861 accgcagggc ctcgcgatag gtgttgacca tcgccagcgt gccggcatcc atttcgccgg
     4921 cgacgttgag ctgattgagc gcccgctccc actgcccctg cacgcacaac aattgaaaca
     4981 agaacgtgcg gagcttggcg tcagctggct tcgcccgaac ctgctgggtg agcgcagcca
     5041 gggcctcgtc gagcttgcca tcgcggagca gttcgtcagc cgacattcat gcgctccgtc
     5101 tggatcaact cgccctggac aatcgccacg tcaagcccca accgggtcct tgccgttcca
     5161 aggcttgttc ttcgtacggt cccagccctt gctgccggcg gccttgttgg tggagccgtc
     5221 ccaggtgtga tactgcatcc aaatcgccca gtaccagatg ctgcattctt cggtaggccg
     5281 ctcatcctcg tcgccgctga tgctccaact ggcgataatc gggttggcca ggatgaattc
     5341 caaatacagg tgttgcttcc cttcctggtc ggcgaacagg aagtgcagct tggcctcctt
     5401 tccgagcgac ccgccggcga tgccggccga catcaggtag gtcgaagcaa cgtcgagcgt
     5461 cttattgacg ctgatcggcg ggatgtcggc cacgccgacg ttcacgtcca gcgtgccggc
     5521 cttcgcgctt tccgaaaacg tccgctcgat gttccagctg acgctggtgc acgctatcca
     5581 attcgtatga tctgcgatct tcgaatcgcc cgggcacttg tccaaatgca acagaatcat
     5641 gccgtcttcc tttcgtgtgg ggagtcagcc gggaggcaga agcacgaaat ccgaagttcg
     5701 aagcacgaaa ccaattcaaa tgactgaacg caaaatgctc taagcgagag aacagcctgc
     5761 cgtcattgga acttcgtgcc tggcgcttga gtcgaattcg tgcttcgagt tttgtcgtta
     5821 gccgcctttg cccgagggca gcttcgagac cagccgcagc gaaacggtca ggccttcgag
     5881 ctggtaatgc gggcggagga aaatcttgga agtgtagtag ccgggattgc cctcgatctc
     5941 ttcgacggtc acctcggcgg cggccagggg cttgcgggcc ttgtcctctt cggtggccat
     6001 cgccgggttc ggctcgatat agttggcgat ccaactgttg agccaggact gcatctggtc
     6061 ccgttccttg aacgagccga tcttgtcgcg aacgatgcac ttcagatagt gggcgaagcg
     6121 gcaggtcgcg aacaggtagg gcaggcgggc cgagaggttg gcgttggcgg tggcgtccgg
     6181 atcgtcgtac tccgccggct tctggagcga ctgggcgccg atgaacgccg cgaagtcgga
     6241 attcttcttg tgcaccagcg gcatgaagcc gttcttggcc agctccgctt cgcggcggtc
     6301 ggcgatggcg atctcggtcg ggcacttcat ctccacgccg ccgtcgtcgg tcggaaaagt
     6361 gtggcagggg agcccttcga ccgcgcctcc cgactcgatc ccgcgaatcc gcgagcacca
     6421 gccgtacatc ttgaacgagc ggttgatgtt cgtggccatc gcgtaggccg cgttgcacca
     6481 ggtgtacttg gtgtggtcgg cgccggtgca cgcctcctcg aagtcgaact ccgagacggg
     6541 gtcggttttc gacccgtagg gcaggcgggc caggaaccgc ggcatcgcca ggcccaggta
     6601 gcgagcgtcg tccgattcgc gcagcgaccg ccagggagcg tattccggcg tgccgaagat
     6661 cttggtcagg tcgcggcggt cgcccagctc ctgccaggaa tccatctgca tcagcgtcgg
     6721 ggccgcgccg gtgatgaacg gggcgtgggc cgcggcggaa atcttggcga tctcgcccag
     6781 gatttccacg tccggcgggc tgtgatcgaa gtggtagtcg ccgatcagac agccaaaagg
     6841 ctcgccgccg aactggccgt attcctcttc gtacatcttc ttgaagagcg ggctctggtc
     6901 ccaggcggtg cccttgaact tcttcagcgt cttgccgagg tccttcttgg agatgttgaa
     6961 gacgcggacc ttcagcatct cgtcggtctc ggtgttgttg acgaggtacg acagcccgcg
     7021 ccaggcccct tccagctgct ggaagtcgga gtggtgcagg atctggttga cctgctcggt
     7081 cagcttcttg tcaagcgcgg cgatgatcgc ctggatcgtt ttgatggcgt cgtccgagat
     7141 caccgccgtg ccggccaggg cctgctcggc gagcgtcttg acggcctctt caacggcttc
     7201 cttggcccgg tcgctcttgg gcttgaattc cttgctcagc agcgccgaga agtcgttcaa
     7261 ctcggcggcc tgcgcctgcg gtgcggcttg tgcttggctc atatcggtgg acatcagaac
     7321 tctctcccat tccagtgtgc gttagtcggg tggttattcc gccttctctt cggccttcgg
     7381 ggcggaagcc agggcttgca gcagcgtcgg atccttcagg gccttgccga tcaggtcctc
     7441 ggctccgctc ttgccgtcca tgtaggtcag caggttggcc agctgggtcc gcgtctcgag
     7501 cagtttgctc agcgcgccga ccttcttggc cacggcggcg ggcgagaagt cgtccatgct
     7561 ctcgaacgtg atgtccacgt tcaggttccc ttcgccggtc agcgtgttgg gcacctggaa
     7621 cgccacccgc ggcttcatcg acttcagccg ctcgtcgaag ttgtccacgt cgaagtcgag
     7681 gaacttgcgg tcgccgacgg cgggcagggc ctcggccggc ttgccggaga gatcggccat
     7741 cacgcccatc acgaacggca gttggatttt cttttccgcg ccgtagactt cgacatcgta
     7801 ttcgatctgg acccgcgggg cgcggttgcg ggcgatgaat ttctgactgc tggctttggc
     7861 caagggacga ctcctctaca ggcggtgttg gatagtggcc tccaagccgc cgcgccgaat
     7921 ggggcgctgt gtgctggaag cggcctctgt gccgcctcgg gactgggaag tatgactcac
     7981 aaacgccacg cggctgctgg cgcgggcgcg gttctttcaa atgtcgttgg gggccgcact
     8041 ggacgcttcg ccgtcgctga tcccgccgat cgcgagcgct tgatttactg cgtcgggcgt
     8101 caggtcgcgg agaatctcca aaaagctctt cgacgccagc cgttttgccc ggttcaggaa
     8161 cagtggcacc ggactcgacg gttcgtagcg tttgtaatag tcgcagattt tgtcgagggc
     8221 gcggattacg tcttcgcgac tggcgatgtc gcctgtgagg ggcgcggccg cgccggcctt
     8281 gcgaccgttg ctcgccgcgc cggcggcttg cccattgccg tccgacttat cggcagccgc
     8341 gggagccgcc ggggccgcgt cgtccaagag tccccgctgg gccagcttct ggtttacgag
     8401 cttcgacatc gccgcgatca cgtccatcag cggcttcagc tctggaccgc tgccggcgcc
     8461 aaccctgtcg ttgaaattcg attcaagctg gcgggccatc cccagcgccc gctccagcac
     8521 cttgccggtc gccttcaact cagcgggatc gctgtcggcg aaagcgccgt cgatcgacga
     8581 ctgctcgacc ttcggagtgc ccgccagcgg cggcatttca cccgaggcga tcagcacgtc
     8641 gcggtacgaa aacttgccgg cgccccgcga ggcgaccagc gtggcctcgc ggacgccctt
     8701 gagcgtctcc tgcgcgctag tcaggctggc cagcgaattg acccgctgcg tgggatcgtt
     8761 gtcgtcggga tcgagctgcg ggtagacgcc gtcccagtat ttctcaatga acccggccag
     8821 cacctccagg ccgtcggcca gcccggccag cccgctggtc cgcaccgccg cccgcgtcag
     8881 gcagacggcg atccgcaggt ctttgctcct gggcagcagg gcctgggcct gcgactgcag
     8941 ctggcgccag tccgggtctt ccgccggaat gacggtctcc ccgaactgct gctcgggctt
     9001 gccgaccgcg gcctgctcca tcatcacgta gtcgacgtcg tactccaggt tctccccgca
     9061 gggagcttcc gccgaaacgg gggaaagcaa ggcttcaacg tcaagcgcgc tcatgaaatc
     9121 ggtgatgcag ccggccggca atgcgtaatc gggtgcgaca atccttcgcg acgcttggaa
     9181 tcctaaagag aagcgatttg gctgcctgcc aagacaggtt gtttctaaac gttctcggcg
     9241 gccaacgcaa ggcaatagga ggggaaatgt cagtggtcat tggtcactcg tcatttgtca
     9301 aagaaattcg gtcgcccgac aaatgaccaa tgaccagtga caaatgacac tcagaataca
     9361 aacctcaaat caaaccgtag cagtgcgtcg taccagccgt cggagccgac cgcgaagacc
     9421 ccgctatagc cgaactcgac gacacccacg tcgccgccgg tgtcgtggac gacccgcagg
     9481 cccggcgcga ggttgaaaat gccgtcgccg cggacatcga ccggcaggcc gccgggaaac
     9541 gggatgaact ggccgtccag cacccagatg ttcgtgaact ccagcgtcgg gatgtaggcc
     9601 gtcgtgtcgg tttcatacca gacgtggctg acgccgatgc cccacttgag gagcggcccg
     9661 gcatagcccg gcgtgcccgg caccgggaac gtcatcttca gctcgctgtg caaaaacgtt
     9721 atgtcgctat agcggtaccg ccagagcatg cccggctcca tcgacacgtg gcccgtgccc
     9781 aggccctttt tggcgttgcc gctgttgaag accgtcctta gcagctgtgt catctgccag
     9841 cggttgccgt cgatcagccg ggtcttttgc accaattcga tgtcgccgat gccgcccgtg
     9901 ttgtcgttct gctcggggtt cagcaggcgg atcggcacgt cggtggccag cgagaaggcg
     9961 ttgctgccgg tctccaagcg gaggcggaac tcctggtaat cgaccgagct ttccggcaac
    10021 aacggcccgc ggcccggtcg ggcccagaag gcctcggccc ggtccggctt cgtcaggtcg
    10081 taaaccgcct gcgtccgcca gaggaaattg ccgaacggct gcgtgatgtc gatctcaaac
    10141 ggggcgtacg ccacccgctc gcgccccagg cccgcctcgc ccgtcggcga gcggacgtgc
    10201 tcagtccacg tgtcccagcc gtgctcgatc tggtcccacg gcaccagcgg ctcgccgctg
    10261 agccagatgt cgctgggttc gccgcgcaag agcggcagca tcggcttggc gtccagcggc
    10321 ccgccgggcc agccgccgtc ggccggcggc ggcggcggca gcgattcatc ctgaatcgtc
    10381 gcgctgaccg gcgcgactgc gccaatggcg gctactgccg gtggctgcac gggcggcgga
    10441 acataagttg tgcccgatac gttcggctgc acgaccagcc gcccgctggc atcgacctgc
    10501 gggtttggca ggttgacgac cacctgggcc gcggccacgc gcgcagccac cagcagcagc
    10561 actgccagca gcggcctgcg aatagcggtc gatcgtttgg acggcggagt catcaagcgc
    10621 agagcctgcg cgcggaagtc caggacatct ccgaaggtat cgaccagcgg gagcttaccg
    10681 gtcaaaccaa ctttgccgga tttgacgacg ctagcggcac tatccttttt tcaccaaacc
    10741 cagccagcca ccgaccgtgg gaaagacgtc gttgtagacg gcgtagccgg cgacggcagt
    10801 gatgagcaca aaggcggtcc agagcagcag gtcggaggcg agcgaaggcg ccagccgctt
    10861 gtcggtgcga aagtagcgca aatagagcgc agcgcccgaa attaccggca gcgtcacggc
    10921 ttgcgcatag ccgccgacgg tgaccatcat cttgggttcg cgaaacaaca ggtacaggat
    10981 cagtgccaaa gtcggaaaga aaacgcagaa tcgccgaatc cagcggggcc gcttctgggg
    11041 atcgtcgaaa cggaccgcgc ccagcagccc caggaagtcg gtcgagagcc gagcgtggcc
    11101 ggccgaggcg acgtagagcg ttttgaagag caccgcccag gccccgatca tgaacaggat
    11161 tttggtccac gcgccgaaag tcggcacata catctccgcc agcgcgacga ccatctgcgc
    11221 gcccttggga tgcaggtccc gcctgtgcag aacggtcgcg cccagcacgt agaaggcgac
    11281 cgtcgcgacc gtgaacacga ccatactcac ccaggcatcg aggatcatca cccgcagcca
    11341 gcctttcgcc cgctcgaccc acttagggtc gtccgaccgc gggccggtga aacgggcgta
    11401 gcccttttcg aggcaccagt acggataggc gtagagctcg ctcgcgccga cgccggtgat
    11461 cccaaaggcg ctaaaggcgg cggcgatcgc cgccggcggc agcgcggcga tggcgaggac
    11521 gttgaatccc tccttaatgt cggcggggcg aatcgggtag ccggtgctcg gcagcagcag
    11581 cacgcaccca accgtgatga gcgtcacgcc ggccaccagc gccgtcgtca cccactccag
    11641 ccgcttgtaa ccgcccgaga gcaagagcag gatcgccgcc agggcggtga gtaccgacca
    11701 gggatgctcc ggccggtcgc gaatggcttc cgccagcggc gccgacatcg attggagggc
    11761 gccagcgatg gcgggcgaga cttgcggaaa cgccacgttc agggcctggc ccacgctgcc
    11821 caccatcgct cccagctgaa acacgctggc cagcagcatg cagaaccacc accacaccag
    11881 ccagcttgct cccagccgcg ggccgggcag cgaattgagc gccgtcagcg tgggctgtgc
    11941 ggacgagatg gcgtagcggc cgagctcgat ttgcacgaag accttgatga cgcagctgaa
    12001 gagcaccagc cacaggaaga cgaaacccca atccgcgccc agcgacgtcg tgagaatcag
    12061 ctcgcccgag ccgacaatgc tgccggccag cacgatgccg gggccgatct tggccagggc
    12121 cttccacagg ctctgcggcg gctcttgaat tgccgcatcg gtaatcgcat acggatcctc
    12181 gacgaccttt tcgcccatcg caccctcccg cggcccatac actagcccca accgcgggct
    12241 ggcgaaaccg cgggcggcga attttcaccg gaccgggggc ttactttcgg ggcgacggga
    12301 cgagcctggt ctcgccggaa ttgagacgcg actccagccg gggtcgaacg ggctccgaca
    12361 cggcgccgcc catcattgcc cgctcaacat ccaccgggaa tcgcagcccg ggcggaagcg
    12421 gatgtcggta agccggaacg tatggcacag gtcgagtcgg aacagaagcg cgcggctcca
    12481 cggcggccaa ctccttctcg agcgcttcca gctgcctggt gagcactccc atggcggcgc
    12541 ggatcttggc aatgtcgccg cgcagctcat ccgctcgaga ttggagtaat tcctgcgcgg
    12601 ccgccggaga ggccgcagtt acgatgatca gtccagcgag cgccagccag cagcgtctca
    12661 tgggagaatc ctccacgcag ggacaaccgt agtatgaccg ctggaatcga agccactcaa
    12721 gcgcacttgc ggacgacagc tatttcacga tctcggtgaa gccgcgggct cggcctcgag
    12781 caacagaaag ctcaaatgca actcggcgtg tcgcaagtgg cactccagcc actggtcatg
    12841 cgagagcctt tcacccgtcg ggctgcggtg cgtcatccgc tcgccgctgg cgaggcgctg
    12901 caactggcgg cggagattgg cggccgcgtg ctcgagtgtg atcgcgggat cgggttcaac
    12961 gctcgcggct gccggcggat tccgaaagcc ggggcgaaag gccagccgca tgaggagcgg
    13021 ccaggagatc aggcccagga gccacgcctg gcagcgcagc cgcagcggat actggaaggc
    13081 aaagccatcg agcgagccgg cgatgaacag cgccaggtgc tggagtacct gcgcggcggt
    13141 ccagttgccg agcggccgca ctcgacccgc agcggcgctg gcgcaaacgc gatcgacttc
    13201 gctggccagg tcggcgagcg actgcaaaag tatggtccgc ctggattgca cacgggctgt
    13261 gctcaccgca gtcatggtgg gcacagccca ccctactcga gtacaatgca ccggccctcc
    13321 taaactcccg ccaaatcagc ctaccgcgag aagagacttc ccatgctgcg gtgttgccct
    13381 ggtgcgctcg ttttgtgcct ggtcgcggtc agcctgcccg ccgcggaagg cgacctggcg
    13441 gacgcgctgg ccaggattcg ggccgtgcag ccgcgagggg tcgggcatcg cgaggcggca
    13501 gcggccgcga gagtcgtcgc cgcggcccgg gcgagcgcgt tgccgcagat tctggcggcg
    13561 atggacggcg ctggcccgat cgccgagaac tggctccgcg gcgcggccga agcggtggcc
    13621 cagcgcggcg gaaagcagtt gcccgtggcc ggcctggagg cgttcctggc cgatacgaaa
    13681 cattcgcccc gcggacgccg gctggcctat gagctgatag ccgccgtcga tcccacggcc
    13741 gagtcgcggc tgattcccac gttgctcgac gatccgagcc tggagctgcg ccgtgacgcc
    13801 gtcgcgcagg tgctggccgc ggccggcaaa gcggaaggta aagaggccgc gcccctgtat
    13861 gccaaggcct tcgcgcattc gcgcgacctg gaccagatca agctggcctt cgacaagctc
    13921 aaggagctgg gcacgccccc cgacatcgcc cgccacatgg gctacgtccg ccaatggcgg
    13981 ctgatcggtc ccttcgacaa cgtcgaggac gccggctggg acaaggcgta cccgcccgaa
    14041 acggcgatcg acctggcggc ggaactgtcg ggccaaaagg gccgcgtaaa atggatcagc
    14101 cacgcctcga ccgacgacta tgggctggtc gagctcacca aggcgctcga caagcataag
    14161 ggcgcggtca cctatgccta cgccgagttc gtcgccgagc gcgagcagcc ctgcgagctg
    14221 cggctctcgt cgccgaacgc caacaaggtc tggctgaacg gcgagctgct ttcggcgaac
    14281 cacgtctatc acgccaacga cccgctcgat caatacatcg cccgcggcaa gctgcggaaa
    14341 gggccgaacg cgatcctggt gaaggtctgt cagaacgagc aggaggagga ttgggcccag
    14401 cggtgggaat tcaagctgcg agtttgcgac acgattggga cggcgatcct ggggaagggt
    14461 tcagggttcg gcgttcaggg ttctggagga taagaatgcg gattcgcgcg accaaacacc
    14521 aaacatccat gcccaaaacc ccgctcctct tcgtgctgct attcgccccc accctgtccg
    14581 ccgccgattg gctgcaattc cgcggcgagg gcaaccgggg cgtggcggag aatgaaaggc
    14641 tgccgacgaa atttggtccg gacgagaagg tggcctggaa ggcggcgctg ccgggcaagg
    14701 ccgcctgcgg gccgatcgtg atcggcgacc gcgtggtcgt gacggcggcc tccggccccg
    14761 tggttcagga tcgcctgcac gttctctgcc tggatgcgaa gaccggccag cagctttggc
    14821 agcggcaatt ctgggcgacg ggccgcccgc atcatcaccc gatgagctcc aacgccgctc
    14881 cgactcccgc cagcgacggg aagaggattt ttgcgttcta ttcctccaac gacgtggtct
    14941 gcctcgacct ggacggcaac ctgctctggt atcgcggcct ggggcacgac tatccgaaca
    15001 cgggcaacga catcggcatg tccgcctcgc cgctggtcgt cgagggcacc gtcgtcgtgc
    15061 aggtcgagtg tcagggggat tcgttcgcgg cgggcctgga caccgccact ggcgaaaacc
    15121 gctggagaat caaccggccg catgcgccca attgggtttc gccggcggcc ctccgccgcg
    15181 cggatgggac gcaagtcgtc ctgctgcaag ccgccgatgg cctgaccgcg cacgagccca
    15241 agacgggcaa gcaactctgg aagtacgaag ccgattgctc atccattccg tcgccgacca
    15301 cggccggcga tcggatcttt ctgccagccg ccggcatgac ggtgctcgaa gcgccggcca
    15361 actccagcgc cccatcgctg ctctgggact ccaacaagct cgcccccggg aatgccagcc
    15421 ccgtgatcga cggcgagcgg ctgtacgtcc tgtcgcgggc cggtgtgctg gccgcggccg
    15481 acgcgaagac cggcgacgtg aagtggcaga cccgcctcaa gggcacgttc tgggccacgc
    15541 cggtcgtggc cggcgggcat gcctactgcg ccaaccagga cggcgaatgc ctggtggtcc
    15601 agctgggcga gaagggcgaa atcgtccaca ccgccgcgtt cggcgagccc ttcctcggta
    15661 cgcccgcaat cgccggcggc gctttgtatt tgcgtggcga gaagcacttg tggaaaatcg
    15721 ggaattgaca ccaacccgac gcgcaagcga gggagagaca atggccaacg aaatccaact
    15781 gaagctcctc aatgagcacg gccgcaagga aatctttttg agcctggccc gcgtgccggg
    15841 cagcagccgc gtcttgtgcg gggcctccga cggcaacgtc tatgacctcg acgcgctggc
    15901 ggaaaagccg gagttcaacg ccctcggcgg ccacgccagc ttcgtgaccg gcgtggcgat
    15961 tgccggcgag ctgctggtca gcggcggtta cgacggcaag ctgctgtggc ggaagttggg
    16021 cggcggcgag atcgttcaca cggtcgaaag cgcccacagc aagtggattc ggaagctcgc
    16081 cgcttcgccc gacggcaagc tggtggccag cgtggccgac gacatggtct gccgcgtctg
    16141 ggaagcggag tccgccaaaa agctgcacga gctgcggggg cacgacgaac aaacaccgaa
    16201 ccattttccc tcgatgctct atacctgcgc cttctcggcc gacgggcggc agctggccac
    16261 cgccgacaag gtcggccgca tctgcctgtg ggacgcggcg agcggcaaca agttgcggca
    16321 gctcgaagcg ccgggctttt acacctggga tcccaagcaa cggatccact caatcggcgg
    16381 cacgcgggcg gtcgcctttt cgcccgatgg caagcagctg gtggcgggcg gcatcggcac
    16441 gatcggcaac atcgaccacc tcgacggccc ggcgcgggtc gagctgtttg aagccgagag
    16501 cggccagagc cagcacgtct tttccggcga cgccaagggc ctcgtcgagc aggtgctgtt
    16561 tcacccggaa ggcaagtggc tgctggccgt cggcggcgac aacggcggcc tgatccaggc
    16621 ctacgaccta gccgagaaga aggtcacgcg gagcgacaag gccccgatgc acatccacgc
//
LOCUS       DASRJB010000755         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3323325, whole genome shotgun sequence.
ACCESSION   DASRJB010000755 DASRJB010000000
VERSION     DASRJB010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323325"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..2221)
                     /locus_tag="VFB80_19975"
     CDS             complement(452..2221)
                     /locus_tag="VFB80_19975"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN36124.1"
                     /translation="MLLFTLANERQRRSIQHNEGPIEFGRLQQGQCARIVVDDPFTSR
                     DQLRIEELPTGDIRVANLGSPVTLPDGTQLNVGAARRFTPPVRLVFGCSTLEVGLIPS
                     ADAPSTGMQTIARPAGGDTGSVKQIFQKGGAPAPEVLAQWFEALLNVQRAAPGSNEFY
                     ADTARAVVELIGLDRGLVILRRRDVWEVIGSCSAANEGSQTKADNRQFSQRVLLQVLA
                     EKRTFYQSFQETGAAQSLMGIEAVVASPVFDEKNAVVGVVYGSRDMRTATGHKGITPL
                     EAQFLQVLAGAVSTGLSRLAREADAARARVQFEQFFSPELARALERDAQILAAQQREL
                     TLLFADLRGFSKIAERIGAKETYQLLADILDRLTNQVMDHGGVVIDYYGDGLAAMWNA
                     PTDTPHHAQRGVQAALAMQGELPAINSVWAEKLGGLIRLGLGLHTGTAQIGNSGSRRR
                     LKYGPRGHAVNLTSRVEAATKVFGVACLMTGATRASLRQPVPLRRVCRGRLTGMSDPV
                     DLFELGVFSDHPTWLAQRDKYEAALALYERQNPSECLAACRAFQAEFGVSDGPTNWLL
                     KHAEQRHAAGDESYDPVFSVETK"
     gene            complement(2366..3403)
                     /locus_tag="VFB80_19980"
     CDS             complement(2366..3403)
                     /locus_tag="VFB80_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36125.1"
                     /translation="MTTNIVFRSGATVLCLALAALLQRTVSGQQPAAKAGMKLPDMKQ
                     PVMHGTPEADAILAAMQVFPPDNPWNADVSKWPRHKDSDAMVASIGPDKPLRYNIDMA
                     FVLVPPTQPKIDLQVVEYPDESDKGPYPIPANIPIEGWPAWYRRDAEDKTLSLEDVQR
                     DKLNAGGDRHAIVVDPYNGRLFEFFGTKRTPAGWQASCAAIFNLGSNDLRTDGWTSAD
                     AAGLPIFPAVIRYDELKAGKITHALRVTVRNTRRAYVAPATHFASNKTDPNLPRMGER
                     LRLKADYDVSGFSPEVQTILKGLQQYGMLVADNGIEWAISCAPDERIPVLHEELRRVK
                     GSAFEVVEAPK"
     gene            3680..4213
                     /locus_tag="VFB80_19985"
     CDS             3680..4213
                     /locus_tag="VFB80_19985"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN36126.1"
                     /translation="MNAALKSVVFGSVLTSLLGVVQIASAAPPHIELRLSPGSRPSLY
                     VGPGTSSTPYITPSPPSPYIMPVAPSSVRLGFYGHFEWGYGMVVDSVPWGTPAQRMGL
                     ESGDVIVAINGLWLRSQSDYYRALAYGGDHARVTVQDVRSGGLVTRTAHLHEDGYAYR
                     SGASGYRSGPSVSLELR"
     gene            4446..5480
                     /locus_tag="VFB80_19990"
     CDS             4446..5480
                     /locus_tag="VFB80_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN36127.1"
                     /translation="MSIGESMQQQAEQFRNRYTSVKEQIGRVIVGHDDIVHGVLTSMF
                     IGGHCLLEGVPGLGKTLLVRTLAECLSLNFNRIQFTPDLMPSDILGTNLINEDEHGRR
                     KFEFQKGPLFTQICLADEINRATPKTQSALLETMQEGTVTIAGVRYELEKPFFVMATQ
                     NPIEQEGTYPLPEAQLDRFLFKLVVGYSGRDELATIVDRTTKGVTIKPEKVMDGQEIK
                     QWQRLIRDVILASHVQDYIVRLILATHPNGAYALPITNQYLRWGASPRGAQTLALAAK
                     VRALLEGRYNVSFEDVRRVYLPALRHRVILNFEAQAEGLDTDHVLLEVLEKLPEKASD
                     EAPVKAALAK"
     gene            complement(5481..>6672)
                     /locus_tag="VFB80_19995"
     CDS             complement(5481..>6672)
                     /locus_tag="VFB80_19995"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN36128.1"
                     /translation="DPLADQTVSVTTLDNDVAAFTIVQSGGTTQVSETVPSTDTFTVV
                     LNVQPLGNVVFTVTSGDLSEATVSPSPLTFTPGDWNVPKTVTVSGVDDAVADGSQVSI
                     VTVSVDDASSDNGFDPLPDQTVSVTTLDNDDAIAPTVDIVDVTPDPRNTPASDVLIAF
                     SEPVLGFDLADVSLRRNGQIVPLAGATLEGTGAAYTLRLVGAAALKGNYLLTLTAAGS
                     GIADVAGNPLAADAQDAWRMTSPWHNVVAPLNTDGFPGVAPLDVLVIVNEVNNRGFSD
                     PITGLLPPVSQTLGPPPFVDANDDGFVTAIDALLVINFLNFGEGEGESPPAGRSAAAQ
                     QETADRGKGEQAPAIEYVSPAGNSPPGRSRYPQPAEFVIPDALLDLLATDVGRRVRGR
                     RNPR"
BASE COUNT         1101 a         2170 c         2226 g         1175 t
ORIGIN      
        1 cggttcgatc cgcggtgcag tggctcggtc ggagaccggc cacaacaact ggctggcgga
       61 aatgtgaaca ttggtatggt atttgcactg aggttccgat cgcgcccgga cctgccaatt
      121 tggcgctggc cggctggcga cgcgcgcggc gaaaggaggc tccgcgatag acttcgcagg
      181 taaacctcgg agctgtaagt gtttatgccg caagtggtta cgctctaaca ctaatgtctg
      241 cagggggcca cagaatcgca ttcgggccac aaatcccagg aatttcgaca gttttgaccc
      301 tggcgagctt tggaacgacc ttataagaac tacttaagcg gttttggagc gctcgccgca
      361 accctatgtg ccgtaatgct ttgcggctag attggcgtaa cgaggcgcga ggcgcatagc
      421 tcaaccaggg ggtcggcata agcggctgcg tctacttcgt ctcgaccgag aagaccgggt
      481 cgtacgattc gtcgcccgcg gcgtgacgct gctcagcgtg cttgagcagc cagttggtcg
      541 ggccgtcgct gaccccaaat tccgcctgga acgcgcggca ggcggccagg cattcggacg
      601 gattctgccg ctcgtatagc gccagcgccg cctcgtattt gtcgcgctgg gccagccacg
      661 tcggatgatc gctgaacaca cccagctcga acagatcgac cgggtcgctc atgcccgtca
      721 accgcccgcg gcagacgcgc cgcagcggca ccggctgccg caggctggcc cgcgtggccc
      781 cggtcatgag gcacgccacg ccgaagacct tcgtggccgc ctcgacccgg ctggtcagat
      841 tcacggcgtg gccgcggggg ccatatttga gccgccgccg gctgccggag ttgccgatct
      901 gggccgtacc cgtatgcagg ccgaggccga ggcggatcag gccgccgagc ttctcggccc
      961 agacgctgtt gatggcgggc aattcgccct gcatggcgag ggcggcctgc acgccgcgct
     1021 gggcgtggtg cggcgtgtcg gtcggcgcgt tccacatcgc cgccaggccg tcgccgtagt
     1081 aatcgatcac cacgccgccg tggtccatca cctggttggt cagccggtcg agaatgtcgg
     1141 ccagcagctg gtaggtttct ttcgcgccga ttcgctcggc gatcttggag aacccgcgca
     1201 agtcggcgaa gagcagcgtc agctcgcgtt gctgggcggc gaggatttgt gcatcgcgtt
     1261 ccagggcccg ggccagctcg ggcgagaaga actgctcgaa ttgcacgcgg gcccgggcgg
     1321 cgtcggcctc gcgggccagg cgggacaggc cggtgctcac cgcgccggcc agcacttgca
     1381 ggaactgggc ctccagcggc gtgatgccct tgtggccggt ggccgtccgc atgtcgcgcg
     1441 agccgtagac cacgccgacc accgcgttct tctcgtcaaa cacgggcgag gccacgaccg
     1501 cctcgatgcc catcaggctc tgggccgcgc cggtttcctg gaacgattga tagaaggtgc
     1561 gcttctcggc cagcacctgc agcagcaccc gctggctgaa ctgccggttg tcggctttcg
     1621 tctggctgcc ctcgttggcc gcgctgcagc tgccaatgac ctcccacacg tcgcgccgcc
     1681 gcaggatcac gaggccgcgg tcgaggccga tcagctcgac gacggcgcgg gccgtgtcgg
     1741 catagaattc gttcgagccg ggcgcggccc gctgtacgtt aagcagcgcc tcgaaccact
     1801 gggccagcac ttcgggcgcg ggtgcgccgc ctttctggaa gatctgcttg accgagccgg
     1861 tgtcgccgcc ggccggtcgg gcgatcgtct gcattcccgt gctcggcgcg tcggcgctgg
     1921 gaatcaggcc gacctcgagc gtcgagcagc cgaagaccag ccgcactggc ggcgtgaagc
     1981 ggcgggccgc tccgacattg agctgcgtgc cgtcgggcag agtgaccggc gagcccaggt
     2041 tggcgacgcg gatgtcgccg gtgggcagct cctcgatccg cagctggtcg cgggaagtga
     2101 agggatcgtc cacgacgatc cgcgcgcact gcccctgctg gagccggccg aactcgatcg
     2161 gcccctcgtt gtgctgaatc gaccgtcgct gccgctcgtt ggcgagcgtg aacagcagca
     2221 ttccgccctc cctgcgccca caagcaaccc gctcggccct cgtggccgag gttccatgat
     2281 agtccgactc gccaaacgct gctgcgcgaa ttcgatggtc cgctttgacg ccaagtctct
     2341 gcgcctttgc ggcaaatcga gagtctcact taggggcttc gacgacttcg aaggcggagc
     2401 ccttgacccg ccgcagctct tcgtggagca ccgggattcg ctcgtcggga gcgcagctga
     2461 tcgcccactc gatgccgttg tcggcgacga gcatgccgta ttgctggagg cctttgagaa
     2521 tcgtctgcac ttcgggcgag aaaccgctga cgtcgtagtc ggccttcagc cgcaggcgct
     2581 cgcccatccg cggcagattc gggtcggtct tgttgctagc aaaatgcgtg gcgggggcga
     2641 cgtaggcccg tcgcgtgttg cggaccgtca cccgcagggc gtgcgtgatc ttgccggcct
     2701 tgagctcgtc gtagcggatc accgcgggga aaatcggcag gcccgccgcg tcggcgctgg
     2761 tccagccgtc ggtcctcagg tcgttgctgc cgaggttgaa gatcgccgcg cacgaggctt
     2821 gccagccggc gggggtccgc ttggtgccga agaactcgaa caggcggccg ttgtacggat
     2881 cgacgacgat cgcgtggcgg tcgccgccgg cgttgagctt gtcgcgctgc acatcctcga
     2941 gcgagagcgt cttgtcctcg gcgtcgcggc ggtaccaggc aggccagccc tcgatcggta
     3001 tgttcgccgg aatcggatag ggccctttgt ccgactcgtc cgggtactcg acgacttgca
     3061 ggtcgatctt gggctgtgtg ggcggcacga ggacgaacgc catgtcgatg ttgtagcgca
     3121 gcggcttgtc cggtccgatc gaggccacca tcgcgtccga gtccttgtgc cgcggccact
     3181 tcgagacgtc ggcattccag gggttgtccg gcggaaagac ctgcatcgcg gccaggatgg
     3241 cgtcggcttc gggcgtgccg tgcatcactg gctgcttcat gtccggcagc ttcatccccg
     3301 ccttggccgc cggctgctgg ccgctaacgg ttcgttgaag cagcgcggcg agcgccaggc
     3361 agaggactgt cgcgccagac cgaaaaacga tgttcgtcgt catgatcagc tcctgaggga
     3421 agcacttcgc atccgggcat gatacgccat ccgtctcagc ctgagttggc tggagtcaga
     3481 atgacggcgc tcgtgctggc agctgttgct gtttcgattt gcaggcggct aagcgcattt
     3541 tcgtaagtgt tgttacagca ggcggttgcg acgatccccg gacacaccaa gtggctttgg
     3601 cacggcacgc gcatttacag ctggcaccgg cgaatcagac cttcgccgca tctaccaacg
     3661 accagaaagc gagaatccga tgaacgccgc cctgaagtcc gtcgtgtttg gaagcgtcct
     3721 gaccagcctg ctcggagtcg tgcagattgc ctccgccgct cctccgcaca tcgagctgcg
     3781 gctgtcgccc ggctcccgtc cgtcgctcta cgtcgggccc ggtaccagct cgacgcccta
     3841 catcacgccc agccccccgt cgccttacat catgccggtc gctccgtcca gcgtgcggct
     3901 gggcttttac gggcacttcg aatggggcta cggcatggtg gtggactcgg tgccgtgggg
     3961 gacgcccgcc cagcggatgg gcctggaaag cggcgacgtg atcgtcgcga tcaacggcct
     4021 ctggctgcgg tcgcagagcg actactaccg ggccttggct tatggcggcg accacgcccg
     4081 cgttacggtg caggacgtcc gcagcggcgg cctggtcacg cggaccgccc acctgcacga
     4141 ggacggctac gcgtaccgca gcggcgccag tggctatcgc tcgggcccca gcgtgtcgct
     4201 ggaactgcga taatcgatcg attgccgacc gcggagccgc aatccggctc cgcggtcttt
     4261 tgcgcttgca tcccgcgcca ataattgaac gggaagagcg aatatggccg atgcgggagg
     4321 gcgaggctcc tgccgagccg gctcagcggg agcttcgccc tcccaatcgg ccccgaccct
     4381 agccccccgc cgctccatct gatagtcata atgtctcaaa tcctggcgtc acctgaggag
     4441 cattcatgag tatcggcgag tcgatgcagc agcaggcgga gcagtttcgg aatcggtaca
     4501 cgtcggtaaa ggagcagatc gggcgggtca tcgttggcca cgacgacatc gttcacggcg
     4561 tgctgacgag catgttcatc ggcggccatt gcctgctgga aggcgtcccc ggcctgggca
     4621 aaacgctgct cgttcgcacg ctggccgagt gcctcagcct gaacttcaac cgcattcagt
     4681 tcacgcccga cctgatgccg tcggacatcc tcggcacgaa cttgatcaac gaggacgagc
     4741 acggccgccg gaagttcgag tttcagaagg gcccgctctt cacgcagatt tgcctggcgg
     4801 acgaaatcaa ccgggccacg cccaagacgc agtcagccct cctggaaacg atgcaggaag
     4861 gcaccgtcac gatcgccggc gtgcggtatg agctggagaa gccgttcttc gtgatggcca
     4921 cgcagaaccc gattgagcag gaaggcacct acccgctgcc ggaagcccag ctcgaccggt
     4981 tcctgttcaa gctggtcgtc ggctacagcg gccgcgacga actggccacg atcgtcgatc
     5041 gcacgaccaa gggcgtcacg atcaagcccg agaaggtgat ggacggccag gagatcaagc
     5101 agtggcagcg gctgattcgc gacgtgatcc tggcctcgca cgtgcaggac tacatcgtac
     5161 ggctgatcct ggccacgcac cccaatgggg cctatgccct gccgatcacc aaccagtatc
     5221 tccgttgggg cgctagcccc cgcggcgcac agacgctggc cctggccgcc aaggtccggg
     5281 cgttgttgga aggccgctac aacgtcagct tcgaagacgt gcggcgggtc tacctgcccg
     5341 cgctgcggca ccgcgtgatc ttgaacttcg aagcccaggc cgaagggctc gacaccgacc
     5401 acgtgctgct ggaagtcctc gaaaagctgc ccgagaaagc cagcgacgaa gcgccggtca
     5461 aggcggcgct ggcgaagtag ttagcgcggg ttgcgccgcc cgcgcactcg ccggccaaca
     5521 tcggtggcca gcaggtccag aagcgcgtcg ggaatgacaa attccgccgg ctgcggatag
     5581 cgcgaacgcc ctgggggact gttacccgcc ggcgaaacgt actcaatcgc cggcgcttgt
     5641 tcgcctttgc cgcggtccgc agtttcctgc tgggcggccg cggaccgacc ggcgggcggc
     5701 gactcgccct ctccttcgcc gaagttcagg aaattgatca cgagcagggc atcgatcgcc
     5761 gtcacgaaac catcgtcgtt cgcgtccaca aagggcggcg gcccaagcgt ctgggaaacc
     5821 ggcggcagca ggcccgtgat tggatcggaa aatccgcggt tgttgacctc gttgacgatg
     5881 acgagcacgt ccagcggcgc gacgcccgga aagccatcag tattgagggg cgccacgacg
     5941 ttgtgccagg gactggtcat ccgccaggcg tcctgcgcgt cagcagccag ggggttgccg
     6001 gcaacatcgg caattccgga cccggctgcc gtcagggtga gcaggtagtt ccctttcaag
     6061 gcggcggccc cgaccaggcg gagcgtgtac gcagcgccgg tcccttccag cgtcgcgccc
     6121 gccaggggca cgatctggcc atttcggcgg agcgacacgt ccgccaggtc gaagccgagg
     6181 acgggttcgc tgaaggcgat cagcacatcg ctggcgggcg tgttgcgcgg atcgggcgtc
     6241 acatcgacga tgtccaccgt cggggcgatc gcgtcgtcgt tgtcgagcgt agtaacgctg
     6301 actgtctggt cgggcagcgg atcgaagccg ttgtcgctgc tggcatcatc gaccgagacg
     6361 gtgacaatcg agacctggct gccgtccgcc acggcgtcgt ccacaccgct gacggttacg
     6421 gtcttgggga cgttccagtc gcccggcgtg aaggtcagcg ggctggggct gaccgtggct
     6481 tcgctcagat ccccactggt cacggtgaag accacattgc ccagcggctg cacgttgagg
     6541 accaccgtga aggtgtcggt gctgggcacc gtctcgctga cctgcgtcgt accgccgctc
     6601 tggacaatcg tgaacgcggc cacgtcgttg tcgagcgtgg tcacgcttac cgtctgatcc
     6661 gcgagcggat cg
//
LOCUS       DASRJB010000756         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3323353, whole genome shotgun sequence.
ACCESSION   DASRJB010000756 DASRJB010000000
VERSION     DASRJB010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323353"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFB80_20000"
     CDS             <1..1127
                     /locus_tag="VFB80_20000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN36129.1"
                     /translation="RPLDPVSWQNGGIPTKDDVDPVTQVNDPRKREILEALATLDWAK
                     LTNQQRIDLLRVYGVTFNRLCRPDSAQRQELLARFDPLYPAKGRELNAELCQLLVYLE
                     APSAPAKTMKLLAGAPTQEEQIDYVRALRIQSGGWTPELRKEYFSWFLKAANYKGGNS
                     LAGFFRNMKNDALATLTADERTALAPILSAQPAAQAVAVAPPRPFVKKWTMEELLPLV
                     EKNMMGRDYDRGRAMFAAANCFACHRFAGEGGSFGPDLSGLAGRFSNRDLLESVVEPS
                     KVISDQYQAVVIRTTDGRTITGRIINLQGDRMTVNTDMLNPGQGGSVGVRSGDIEAMQ
                     PSSTSMMPQGLLDTMNQDEVLDLLAFLLSRGERESSLFRR"
     gene            1189..1839
                     /locus_tag="VFB80_20005"
     CDS             1189..1839
                     /locus_tag="VFB80_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN36130.1"
                     /translation="MVHGYHVILGAYGYWLPNDPRGTWSDFVGKWELLRFGKSKRSMD
                     RRALEELTCEEREERERARESLEYPAVQFSGQQARGIGRGFSFVSQKRGFTIWACSIL
                     PEHTHLVIARHRYRVESIINALKGESTRQLVSERLHRLSQYAAPGKRPPRMWSTGEWK
                     VFLDSEEAIESAIAYVVQNPVEESKPLQRWNFVASFQGLDAGWTTYHDERPRPPKV"
     gene            complement(1900..2778)
                     /gene="prmC"
                     /locus_tag="VFB80_20010"
     CDS             complement(1900..2778)
                     /gene="prmC"
                     /locus_tag="VFB80_20010"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZN36131.1"
                     /translation="MSEPWTVLRLLTWTTDYLKAHGSDSPRLDAEVLLAHARGCERIM
                     LYAAFDQVVADDVRAKFRELVKRRAEGAPVAYLVGKREFYSLSLRVTPDVLIPRPETE
                     LVVVETIESGSKFKVQGSKLEVADVGTGSGAIAVAVARHAPQCRVTAIDNNPAALAIA
                     RENAAANGVAERIEVVLGDLLGGLPAQPRFAVIASNPPYVSESEFATLAPSVKNHEPR
                     QALVAGPEGTEVIERLIPQAAERLLPGGWLIVELSPMIAARVVELIAKSGRYEPATLL
                     KDLAGLQRVVKARRFV"
     gene            complement(2891..3973)
                     /gene="prfA"
                     /locus_tag="VFB80_20015"
     CDS             complement(2891..3973)
                     /gene="prfA"
                     /locus_tag="VFB80_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZN36132.1"
                     /translation="MSSIREQLDSQLRRFEELETKMADPAIQADGAKFSLVAREHGSL
                     VKQAMKYRRFKSLVAEIAELKQMSETGGPDEKELAEAELPAAREKREALWSELLDMTV
                     GGEDANRTRCVMEIRGGTGGEEAALFAHDLFQMYKHHAEKKGWKVEIMEASPTDRGGF
                     SKITLAFEGEGVYRELRYEGGTHRVQRVPETEAKGRVHTSAATVAVLPEPEDVEINLK
                     QDDYRLDRFCASGPGGQHVNKTESAVRLTHHETGIVVSMQDEKSQHKNLAKALRILKS
                     RLYEHYQKQEAAKRAAERKTMVGSGDRSDKIRTYNFPDNRLTDHRIGFTIYKLDQIIR
                     GDFQPVTDALIEHDRNQLRDQMGGLE"
     gene            complement(4070..4339)
                     /gene="rpmE"
                     /locus_tag="VFB80_20020"
     CDS             complement(4070..4339)
                     /gene="rpmE"
                     /locus_tag="VFB80_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZN36133.1"
                     /translation="MKDGIHPNYVLATVTCSCGNTFQTRATKRELKIDICSACHPFFT
                     GKLKYVDTAGRIEKFQSKFAAGTYTSLKKPAKAPKRKPTPAGEEA"
     gene            4542..>5214
                     /gene="purH"
                     /locus_tag="VFB80_20025"
     CDS             4542..>5214
                     /gene="purH"
                     /locus_tag="VFB80_20025"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909041.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN36134.1"
                     /translation="MDRIPIRRAIISVSDKSGLAELGRELAAARVEIYSTGGTRQFLL
                     DQGLQAIDVAAYTAFPEMMHGRVKTLHPKVFGGILCRRDHPEDLTAAREHGIELLDLV
                     VVNLYPFTQTIAKPGVSAAEAIENIDIGGPSLVRAAAKNHAFTAIATGPGQYAEVTAA
                     LREGGTTLALRQKLAAAAFAHTAAYDAAIANWFGGQLQGELFPPTLHLALKRQTELRY
                     GENAHQ"
BASE COUNT          888 a         1667 c         1703 g          956 t
ORIGIN      
        1 accggccgct cgatccggtc tcctggcaga acggcggcat ccctacgaag gacgatgtcg
       61 atccggtcac gcaggtcaac gacccgcgga agcgcgagat tctcgaggcg ctggcgacgc
      121 tcgactgggc gaagctgacg aaccagcagc ggatcgacct gctgcgggtg tatggcgtga
      181 cgttcaaccg gctctgccgg cccgactcgg cccagcggca ggaattgctg gcgcgatttg
      241 acccgctcta tccggccaag ggccgcgagc tgaacgccga gctctgccag ttgctcgtct
      301 atctggaggc gcccagcgcc ccggcgaaga cgatgaagct gctggccggc gcgccgactc
      361 aggaagagca gatagactat gtccgggcgt tgcggattca gagcggcggc tggacgcccg
      421 agctgcggaa ggagtatttc tcgtggttcc tcaaggccgc caactacaag ggcggcaaca
      481 gcctggccgg gttcttccgc aatatgaaga acgacgccct ggcgacgctg acggcggacg
      541 agcgaacggc tcttgcgccg atcttgagcg cgcagccggc ggcgcaggcg gtggcggtcg
      601 cgcccccgcg gcctttcgtg aagaagtgga cgatggagga gctgctgccg ctggtcgaga
      661 aaaacatgat gggccgcgac tatgaccgtg ggcgggcgat gttcgccgcg gccaattgct
      721 ttgcctgcca ccgcttcgcc ggcgagggag gctcgttcgg cccggacctc tccggcctcg
      781 ctgggcggtt cagcaatcgc gacctgctgg aatcggtcgt tgagcccagc aaggtcatca
      841 gcgaccagta tcaggcggtc gtcatccgca ccaccgacgg tcgcaccatc accggccgga
      901 tcatcaactt gcagggggac cgcatgaccg tgaacaccga catgctcaat cccggccagg
      961 gcggcagtgt gggtgtccgc agcggcgaca tcgaagccat gcagccctcg tccacctcga
     1021 tgatgccgca gggcctgctc gacacgatga accaggacga agtcctcgac ctcctggcgt
     1081 tcctgctctc gcgcggcgag cgcgagagca gcctgtttag gcgttagtcg tttaacccgg
     1141 agccggaggc gacggcggct acagcaattc gctcctcagt actgcgaaat ggtccacggc
     1201 tatcacgtca tcctgggggc gtatggctat tggctgccca acgatccgcg gggcacgtgg
     1261 tccgactttg tcggaaagtg ggagctgctg cgattcggga agtcgaagcg gagcatggac
     1321 cggcgggccc tcgaagagtt gacgtgcgag gagcgtgaag agcgcgagcg cgcccgcgag
     1381 tcattggagt atcccgcggt tcagttctca ggccagcaag cccgcggaat tgggcgagga
     1441 ttttccttcg tctctcagaa acgcggtttc acgatttggg cctgctcgat tctgccggag
     1501 catacacatc tggtcattgc ccgccatcgc tatcgcgtcg agtcgatcat taacgcgctc
     1561 aaaggcgaat ccacgcggca gctcgtcagt gagaggctcc atcgtctctc ccaatacgcc
     1621 gcgccaggca agcggccgcc acgaatgtgg tctaccggcg aatggaaagt gtttctcgac
     1681 tcggaagagg ctattgaaag cgcgatcgct tacgtcgtgc agaatcccgt cgaggaaagc
     1741 aagccgcttc agcgttggaa tttcgtcgcg tcgttccagg gactggacgc cggatggacg
     1801 acctatcacg acgagcggcc taggccgccc aaagtgtagt tgtttaaccc ggagccggag
     1861 gcgacgctgt cccgcaacgc cgtcgcctcc ggctccgggt taaacgaagc gccgcgcttt
     1921 gacgactcgc tgcaggccgg cgaggtcttt gaggagcgtg gcgggctcgt agcggccgct
     1981 cttggcgatc agctcgacga cgcgggcggc gatcatggga ctgagctcga cgatcagcca
     2041 gccgccgggc agcagccgct cggcggcttg cgggattagc cgctcgatga cttcggttcc
     2101 ctccggcccg gcgacgagcg cctgccgcgg ctcgtggttc ttcacgctcg gggccagcgt
     2161 ggcgaactcg ctctcggaga catacggcgg gttgctggca atgaccgcga accgcggctg
     2221 tgcaggcagc ccgccgagca aatcgcccag gacgacctca atccgctcgg caacgccatt
     2281 ggcagctgcg ttttcgcggg cgatcgccag cgcagccggg ttgttgtcga tggccgtcac
     2341 gcggcactgc ggcgcatgcc tggcgacggc gaccgcaatc gccccgctgc ccgtgccgac
     2401 gtcggctacc tccaactttg aaccttgaac cttgaacttt gaaccagact cgatcgtttc
     2461 gacgacgacg agctcggtct ccggccgggg aatcagaaca tccggcgtca cccgaaggct
     2521 cagcgaatag aattcccgct tgccgacgag ataggcgacg ggcgcgcctt cggcccgccg
     2581 ctttaccagt tcgcggaatt tggcccgcac atcgtcggcc acgacctggt cgaacgcagc
     2641 gtaaagcatg atccgctcgc agccgcgagc gtgcgccagc aaaacctccg catccagccg
     2701 cggactgtcc gagccgtgcg ccttcaaata gtccgtcgtc caggtcagca ggcggaggac
     2761 ggtccagggc tcggacatga agggctaggg ctgggggcta gggctaggga gcggacaaag
     2821 ttgcggtgat tgtactgcga ggaaaacggg acagctgcac ttccctagcc ctaaccccca
     2881 gccctagccc tcattccagt ccgcccatct gatcgcgcag ctggttccgg tcgtgctcga
     2941 tgagggcgtc ggtgacgggc tggaagtcgc cgcggatgat ctggtcgagc ttgtagatcg
     3001 tgaagccgat gcggtgatcg gtgaggcggt tgtcggggaa attgtaggtg cggatcttgt
     3061 cgctgcggtc gccgctgccg accatcgtct tccgctcggc ggcccgcttg gcggcttcct
     3121 gcttctggta gtgctcgtac agccgcgact tgagaattcg cagggccttg gccaggttct
     3181 tgtgctggct cttctcgtcc tgcatcgaca cgacgatgcc ggtttcgtgg tgggtcaggc
     3241 ggacggccga ctcggtcttg ttgacgtgct ggccgccggg gccgctggca cagaaccggt
     3301 cgaggcggta gtcgtcctgc ttgaggttga tctcgacgtc ctccggctcc ggcagcacgg
     3361 cgacggtcgc ggcgctggtg tgcacgcggc ccttggcctc agtctccggc acgcgctgca
     3421 cgcggtgcgt tccgccctcg tagcgcagct cgcggtacac gccctctccc tcgaaggcca
     3481 gcgtgatctt gctgaagccg ccgcggtcgg tcgggctggc ctccatgatc tcgaccttcc
     3541 agcccttctt ctcggcgtgg tgcttgtaca tctggaacag gtcgtgggcg aagagggccg
     3601 cctcttcgcc gccggtgccg cctcggattt ccatcacgca gcgggtgcgg ttggcgtcct
     3661 cgccgccgac cgtcatgtcg agcagctcgc tccatagcgc ttcgcgcttc tcgcgggctg
     3721 cgggcagctc cgcctcggcc agctcctttt cgtccggccc gccagtctcg gacatttgtt
     3781 tcagctcggc gatttcggcg accaggctct tgaagcggcg gtacttcatc gcctgtttga
     3841 ccagcgaccc gtgctcgcgg gctaccagcg aaaacttcgc gccgtcggcc tgaatggccg
     3901 gatcggccat tttcgtctcc agctcttcaa atcggcgaag ctggctgtcg agttgttcgc
     3961 gaatggatga catgcgtccg gagagcctgt gatttgatgc ttcgctccgc gcgcagcacc
     4021 aaaccgggaa cgattccaat gatacccgaa tccagaaaat cgccggtagc taagcttcct
     4081 cgccggcggg cgtcggcttc cgcttcggcg ccttggccgg cttcttcaag ctggtatacg
     4141 tgcccgcggc gaacttcgat tggaacttct cgatccggcc ggccgtatcg acgtacttca
     4201 gcttgcccgt aaagaacggg tggcaggccg agcaaatgtc gatcttcagc tcccgcttcg
     4261 tcgcgcgagt ctggaaggtg ttgccgcagc tgcacgtgac ggtcgccagc acgtaattgg
     4321 gatggatgcc gtctttcatg gctcaaactc gcggctgatt caatttgaag ggaatttgta
     4381 agtctatcgg ccagccaccg cgcgggcaag agccgcggcc gcgaggtcgt tactgcttgt
     4441 caggcttaga cttgcggtcg taacgatggg tcagtccttg accgtctgag ccagcagccg
     4501 taaaccagag gctgcaagca ccacaagcca ggaaagagcc cgtggaccgc attccgatcc
     4561 ggcgagcgat cattagcgtc agcgacaaga gcggcctggc cgaattgggc cgcgagctgg
     4621 cggctgctcg cgtcgagatc tacagcacgg gcggcacgcg gcagtttctg ctcgaccagg
     4681 gcctccaggc catcgatgtc gccgcttaca cggcctttcc tgagatgatg cacggccgag
     4741 tgaaaacgct gcatcccaag gtctttggcg gcatcctctg ccgccgcgac cacccggagg
     4801 acttgacggc ggcccgcgag cacggcatcg agctcttgga cctggtcgtt gtcaatctct
     4861 atccattcac ccagacgatt gcgaagccgg gcgtgagcgc ggccgaggcg atcgagaaca
     4921 tcgacatcgg cgggcccagc ctggtgcggg ccgccgcgaa gaaccacgcg ttcacggcaa
     4981 ttgcgaccgg gcccggacaa tacgccgagg tgaccgcagc cctgcgcgaa ggcggcacga
     5041 cgctggccct gcgacagaag ctggccgcgg ctgcgttcgc ccacactgcg gcctacgacg
     5101 cggcgattgc caactggttc ggcggtcagc tgcagggcga actgttcccg ccgaccttgc
     5161 acctggcctt gaagcggcag accgagctgc gctatggcga gaacgcccat cagc
//
LOCUS       DASRJB010000757         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_219413, whole genome shotgun sequence.
ACCESSION   DASRJB010000757 DASRJB010000000
VERSION     DASRJB010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219413"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFB80_20030"
     CDS             <1..371
                     /locus_tag="VFB80_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36135.1"
                     /translation="DELVYFTRVFPHDNLTLETPLVEIEERRYPGHGRRRRWRRDDHV
                     AEDQRLLAVQAVHRFRTTSDLLALLPPDLPPEFHTGHLAEQLGIRRWIAQRIAYCLRK
                     TGAAEICGKCGNALVYRRAA"
     gene            368..1222
                     /locus_tag="VFB80_20035"
     CDS             368..1222
                     /locus_tag="VFB80_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN36136.1"
                     /translation="MNIFQGMESYFAGLAQILVEGGRPEANLARAADAIRAAAAQDCR
                     LVVLPECLDLGWTDPSARDMAQPIPGPHLACLAAATRDCGIYAAAGLVERRGERLYNA
                     AVLLSPAGEILLHYRKINELDIALDLYSVGDKLGVARTELGVLGLNICADNFGDSLAI
                     GHTLARMGAQVILSPSAWAVEAAHDQAREPYGQLWRDAYERLTTLYDLTVLGVSSVGW
                     ITGGPWRGRKCIGCSLAMGPEGTVLAEAPYGESADALLRVLVHPRPPIARGAEFAAAL
                     AKRGYHGS"
     gene            complement(1219..>2062)
                     /locus_tag="VFB80_20040"
     CDS             complement(1219..>2062)
                     /locus_tag="VFB80_20040"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36137.1"
                     /translation="RKLRSSRNPRGFTLVELLVVIAIIGVLVALLLPAVQAARESARR
                     MQCGNNLKQWALATHNYHDVMGVIPTGMTLPGQWIFRAFLLPYVEQSNLNNKIDYSYQ
                     PYCFAFNAAAGANNPSDDPVPLYFCPSELHSKKIYRNYVGADYMTTVYLGVTGGRSDD
                     VFTNDGSFYVNSRIRLANYTDGTSNTVMIGERGLPEDLFWGWALCGGSAYDAFLSMKF
                     GFMRGDAKNSTHLQHFWSYHPGGAQFSRVDGSVSMLKYTTDFNTIVALATRDQGDLVS
                     GSAD"
BASE COUNT          349 a          667 c          684 g          362 t
ORIGIN      
        1 tcgacgagct ggtttatttc acgcgcgtct ttccgcatga caatctcacg ctggaaacgc
       61 ctctggtcga gatcgaggag cggcgctatc ccgggcacgg ccgtcgccgc cgctggcggc
      121 gggacgacca cgtcgcggaa gaccagcggc tgctcgccgt ccaggccgtc caccgcttcc
      181 ggaccacaag cgacttgctc gcgctcttgc cgccggacct gccgccggaa ttccacactg
      241 gccacctggc ggaacagctc ggcatccgcc gctggattgc ccagcgcatc gcctattgcc
      301 tgcgcaaaac cggcgcggcc gaaatctgcg gcaagtgcgg caacgcgctg gtctatcgac
      361 gcgcggcatg aatatattcc agggcatgga aagctatttc gccggtctgg cccagatcct
      421 ggttgagggt ggacgcccgg aggcgaacct cgcgcgagcc gcggacgcca tccgtgcggc
      481 ggccgcgcag gactgccggc tggttgtctt gccggaatgt ttggacttgg gctggaccga
      541 tccgtcggcg cgggacatgg cccagcccat tcccgggccg catttggcct gcctggcggc
      601 ggcgacccgc gactgcggga tctatgcggc cgcgggcctg gtcgagcggc gcggcgagag
      661 gctctataac gcggcggtgc tgctctcgcc tgcaggcgag atcctgctgc actatcgcaa
      721 gatcaacgag ctggatatcg ccctggatct atattcggtc ggcgacaagc tgggcgtggc
      781 ccgcaccgag ctgggcgtgc tgggactcaa catctgcgcc gacaacttcg gcgattcgct
      841 ggccatcggc catacgctgg cccgcatggg ggcgcaggtc atcctctcgc catcggcgtg
      901 ggccgtagag gccgcccacg accaggcccg cgagccctat gggcaactgt ggcgcgacgc
      961 ctacgagagg ctgacaacgc tgtacgacct gactgtcctc ggcgtgagca gcgttggctg
     1021 gatcacgggc ggcccctggc gcggccgcaa gtgcatcggg tgttccctgg cgatgggacc
     1081 ggaaggcacc gtactcgcgg aagcccccta tggcgaatcc gccgacgccc tcctgcgggt
     1141 cctggtgcac ccccggcctc caatagcgcg gggggcggag tttgccgccg ccctggccaa
     1201 gcggggctac cacggaagct agtccgcgga gccggaaacc aggtcgccct ggtcgcgggt
     1261 ggcgagtgcg acgatcgtgt tgaagtccgt cgtgtatttc agcatcgaca ccgagccatc
     1321 gacccgcgag aactgcgcgc cgccgggatg gtagctccaa aaatgctgca agtgcgtgga
     1381 gttcttggcg tctccccgca tgaagccgaa cttcatgctg aggaacgcgt cgtaggcgct
     1441 gccgccgcac aacgcccaac cccaaaacag gtcttccggt agcccccgct cgccgatcat
     1501 tacggtgttc gaggtcccgt ccgtatagtt tgccaggcgg atccggctgt tgacgtagaa
     1561 cgagccgtca ttggtgaaga cgtcgtcgct gcgtccgccc gtgacgccga ggtagaccgt
     1621 cgtcatgtag tcggcgccaa cgtaattgcg gtagatcttc ttcgagtgca gctcgctcgg
     1681 gcagaaatag agtgggactg gatcgtccga cgggttgttg gccccggcgg ccgcgttgaa
     1741 ggcgaagcaa tacggctgat agctgtagtc gatcttgttg ttcagattcg actgctcgac
     1801 atagggcaag aggaacgctc ggaaaatcca ctgcccgggc agggtcatgc cggtgggaat
     1861 cacgcccatc acgtcgtggt agttgtgcgt cgccaaggcc cattgcttga ggttgttgcc
     1921 gcattgcatc cgccgcgccg attcgcgggc cgcctgcacc gcgggcaaga gcagcgccac
     1981 cagtacgccg atgattgcaa tcacaaccag caactccacc agcgtgaagc cgcgcggatt
     2041 tcgggaagac cggagctttc gg
//
LOCUS       DASRJB010000758         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4168989, whole genome shotgun sequence.
ACCESSION   DASRJB010000758 DASRJB010000000
VERSION     DASRJB010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168989"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFB80_20045"
     CDS             complement(<1..472)
                     /locus_tag="VFB80_20045"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN36138.1"
                     /translation="MATGIHAFLDLLAAFGVRQIFGNPGTTEMPLNDALVGDRRFQYI
                     LGLSEVPVMAMADGFAMASRSLGVVNLHISCGLGNAMGMLYNAHREGTPLLVTAGQQD
                     RRLMFEEPILYGQMVEVARPWTKWSCEVQRVQDLPSAVRRAVQTALTPPTGPVFL"
     gene            508..735
                     /locus_tag="VFB80_20050"
     CDS             508..735
                     /locus_tag="VFB80_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36139.1"
                     /translation="MSEGESSAEGRGYAAAVVLALMGVVSLLALPFVPLLLALIEGMT
                     FGTRRVEDFCEQVGIHDELTALYQTIFGIFK"
     gene            822..1661
                     /locus_tag="VFB80_20055"
     CDS             822..1661
                     /locus_tag="VFB80_20055"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN36140.1"
                     /translation="MRVSARVNGEAVAAAEVDREIRQAYGDRKFEGAEQQQVFQAALA
                     QVIDRRLVLAHLTASGQAATSQDLDFATAQFEKDLKSQELTLEAHLKQVGLTREDFRR
                     SLAWKLSWQRYVEKQLTDENLQKFFERYRREYDGSELRVAQILFKLPAEADAAAIAAA
                     KEKAAGLRAEIAAGKFAFAAAAKQHSQAPSAAAGGDIGWIERRRPMPEEFSKLAYSLK
                     PGEVSPPLASPFGVHLITVLEVKPGKRTWQDAAGELRPAVTLYLFRWIADQERAKAKI
                     EYP"
     gene            complement(1674..1916)
                     /locus_tag="VFB80_20060"
     CDS             complement(1674..1916)
                     /locus_tag="VFB80_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36141.1"
                     /translation="MTVQTFRDLLSQRPFKPFRLVMSSGQTYDVRHPEMAWLTRSDIL
                     VGIDDDQDGVPAEFKICSLLHVTAIEPLNSPAPGSR"
     gene            1954..3054
                     /gene="mutY"
                     /locus_tag="VFB80_20065"
     CDS             1954..3054
                     /gene="mutY"
                     /locus_tag="VFB80_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZN36142.1"
                     /translation="MDEPKRATSPLSPRWRRALARKLLDWFAAHARDLPWRRTKDLYA
                     IWVSEIMLQQTQVATVIPYWQRFLSRFPDVASLADADEEDVLRLWEGLGYYRRARQLH
                     AAARKIAREHGGQFPASYSAVRELPGIGRYTAGAILSIGRDQRLPILEANSIRVLSRL
                     AAFEGDPASTAGQKHLWSLAEQILPAKGCGAFNQALMELGSEVCTSKSPACDRCPVAA
                     LCEARRRGLVDSIPRPGKRMNYEDATHVAVVVRDRGRVLLRQCQRGERWAGLWDFPRF
                     EANGHGTLAEQIARQTYELTGVEVAPGERLATFKHGVTRFRITLHCYAARKTSKRRIG
                     QAARWVALPELVDYPLSTTGRKISRLLGKDTE"
     gene            3132..3470
                     /locus_tag="VFB80_20070"
     CDS             3132..3470
                     /locus_tag="VFB80_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36143.1"
                     /translation="MDPSSKLTPQRELAPATKAADLNPYESPHSSGAPTQKIGWLGAS
                     LAAVGALVSALYLANVTGGFLEFSPDNLPGFGNLDEFLFSLLLVYCLQKLGINLLPFM
                     KPGASRGGGS"
BASE COUNT          644 a         1119 c         1208 g          592 t
ORIGIN      
        1 acagaaacac cgggccggtg ggcggcgtga gggcggtctg cacggcgcgg cggaccgcgg
       61 agggcaaatc ctgcacccgc tgcacctcgc acgaccactt cgtccagggc cgcgcgactt
      121 cgaccatctg gccgtacagg atcggctcct cgaacatcag gcggcggtcc tgctggccgg
      181 cggtgacgag cagcggcgtg ccttcgcggt gggcgttgta gagcatgccc atcgcgtttc
      241 ccagcccgca ggagatgtgc aggttgacga cgcccaggct gcgcgaagcc atcgcaaagc
      301 cgtcggccat cgccatcacg ggcacttccg agagacccag gatatattga aagcggcggt
      361 cgccgaccag ggcgtcgttc agcggcatct cggtcgtgcc cggattgccg aaaatctgcc
      421 gcacgccgaa agcagcgagc aggtcgagga aagcgtgaat gccggtggcc atgccgcgat
      481 tgtaccgccc gcggcgggag ttagcgcatg agcgagggcg agtcgagtgc ggagggccgc
      541 ggctatgcgg ccgcggtcgt gctggcgctc atgggagtcg ttagtctgct ggccctgccg
      601 ttcgtgcccc tgctgttggc cttgatcgaa gggatgacgt ttggaacgcg gcgggtcgaa
      661 gacttttgcg aacaggttgg gattcacgac gagctgactg cgctctatca gacgatcttc
      721 ggcatcttca aatgacgcgg ggacaatcga catcgcgatg gctcttcacg ctcagctgcc
      781 tggcctcggt agctccttgc tgggcgcaac cgcagccgga attgcgagtc agtgcgcgcg
      841 tcaacggtga ggccgtggca gcggcggaag ttgatcgcga aattcgccag gcttatggcg
      901 accgcaagtt cgagggcgcc gagcagcagc aagttttcca ggcggcgctg gcccaagtga
      961 tcgaccgccg gctggtgctc gcgcacctca cggcgagcgg ccaggcggcg acgagccagg
     1021 acctcgactt tgcgacggcc caattcgaga aggacctgaa gtcgcaagag ctgacgctcg
     1081 aagcgcacct gaagcaagtc ggcctgacgc gggaggactt tcggcgttcg cttgcctgga
     1141 agctctcctg gcagcgctac gtcgaaaaac agctgaccga cgagaatctg cagaagttct
     1201 tcgagcgcta ccgacgcgaa tacgacggca gcgagctgcg cgtcgcgcag atcctgttca
     1261 agctgccggc cgaggccgac gcagccgcga ttgccgcggc caaggagaag gcggctgggc
     1321 taagagcgga aattgccgcg ggaaagttcg cgttcgcggc tgccgcgaag cagcactcgc
     1381 aggcccccag cgcggcggcc ggcggcgaca tcggctggat cgagcgccgc cgcccgatgc
     1441 cggaggagtt ttccaagctg gcctacagcc tcaagcccgg cgaggtcagc ccgccgctgg
     1501 cgagcccgtt cggcgtgcac ctgattaccg tgctggaagt caagccgggg aaaaggacct
     1561 ggcaggacgc cgccggcgag ctgcgtcccg cggtgacctt gtacctgttc cgctggatcg
     1621 ccgaccagga gcgggcgaag gcgaagatcg agtatccgta gcgcccggga taactaccgg
     1681 gagcccggcg ccggagaatt cagcggctca atggcggtaa cgtgcagcag cgagcaaatc
     1741 ttgaattccg ccggaacacc gtcctggtcg tcgtcgattc caacgagaat gtctgagcgc
     1801 gtgagccagg ccatttccgg atggcggacg tcgtacgtct gcccgctcga catcacgaga
     1861 cgaaagggct tgaacggccg ctgggagagc aaatcgcgaa aagtctgaac ggtcatggcc
     1921 ggctccaggc tctctgctat tttagcgtaa atcatggacg agcccaaacg agcgacctcg
     1981 ccattatctc ctcgctggcg gcgggcgctg gcccgaaaac tcctcgactg gttcgcggcg
     2041 cacgcccgcg atctgccttg gcggcggacg aaagacctgt acgcgatctg ggtcagcgag
     2101 atcatgctcc agcagacgca ggtcgccacg gtgattccct attggcagcg gttcttgagc
     2161 cggtttcccg atgtggcgtc gctggctgac gccgacgagg aggacgtatt gcggctgtgg
     2221 gagggcctgg gctattaccg ccgggcgcgg cagctccacg ccgcggcccg caagatcgct
     2281 cgggagcatg gcggccagtt tcccgcgagc tactccgcgg tccgcgagct gcccggaatc
     2341 ggccgctaca cggccggggc aatcctttcg atcggccgcg atcagcggct gccgatcctg
     2401 gaggccaatt caattcgagt tctcagccgg ctggcggcgt tcgagggcga tccggccagc
     2461 acggccggcc agaagcacct ctggtcgctc gccgagcaaa tcctgccggc caagggctgc
     2521 ggggcgttca accaggcgct catggagctg ggcagcgaag tctgcacttc caagtcgccg
     2581 gcttgcgacc gctgcccagt cgcggcgctc tgtgaggcgc ggcggcgggg cctggtcgat
     2641 tcaataccgc ggccggggaa gcgaatgaac tacgaggacg ccacgcacgt cgccgtggtt
     2701 gtccgcgacc gcgggcgcgt gctcctgcgg cagtgccagc gcggcgagcg gtgggccggc
     2761 ctgtgggatt ttccgcggtt tgaagccaat gggcacggca cgctggccga gcaaatcgcc
     2821 cggcaaacgt acgagctgac cggcgtcgaa gtcgcgcccg gcgagcgctt ggcgaccttc
     2881 aagcacggcg tcacgcggtt tcggatcacc ctgcattgct atgccgccag aaaaacctcg
     2941 aagcgccgca tcggccaggc cgctcgctgg gtcgcactcc cggagctggt cgattacccg
     3001 ctcagcacga ccggacgcaa gatcagccgg ttgctgggca aggatacgga atagaaccgc
     3061 ggattacgcg gaggacgcgg atgggcagac tgttctctgc actgcggaca ctggtttcgt
     3121 agaattatgc tatggatcct tcctctaagc tgacaccgca gcgcgaactt gctccggcca
     3181 ccaaggcggc cgacctcaac ccctacgaat cgccgcacag ttctggcgct ccgacgcaaa
     3241 aaatcggatg gctcggtgcg tcgctggcgg cagtcggagc gctcgtttcg gcgctgtacc
     3301 tggccaacgt gacgggcggc tttctggaat tctcgccgga caatctgccc gggttcggca
     3361 accttgacga attcctgttt tcgctgctgc tcgtctattg cctgcagaag ctaggtatca
     3421 acctgttgcc cttcatgaag cccggcgctt cccgcggagg cggaagctga cagctcaatc
     3481 gtccttccga taaaaccggt tcgccaggac gtgttccagg ccgagcgcga cggcgacgag
     3541 cgcgatcagg aggggataga act
//
LOCUS       DASRJB010000759         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4169084, whole genome shotgun sequence.
ACCESSION   DASRJB010000759 DASRJB010000000
VERSION     DASRJB010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4169084"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1184)
                     /locus_tag="VFB80_20075"
     CDS             complement(180..1184)
                     /locus_tag="VFB80_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZN36144.1"
                     /translation="MSSFDLVSEKADKRAHIADLVQTIKETADKLALDGSTRGDLKIL
                     SRTLRELRYAFKVFAPYRRKRKVTVFGSARTQPDEPAYVQAMDFGRSMAQAGWLVITG
                     AASGIMEAGHRGAGREASMGLNIMLPFEQSANPVIQGDTKLVHMKYFFTRKLMFVKEC
                     DAVVCLPGGFGTLDEAMEVITLLQTGKRDMIPVVLLDAPGGNYWRALHEYVINHLLRA
                     RMISPEDLHLYKLTDSHEAAKAEVLQFFRVYHSMRYVKSDLVLRLQTPLSPELLEAIN
                     REFVDLLTAGHFVQRPALDLERDEADLAHMPRLVFAFNRRNLGRLRLLIDVINRGRVD
                     "
     gene            complement(1380..1628)
                     /locus_tag="VFB80_20080"
     CDS             complement(1380..1628)
                     /locus_tag="VFB80_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36145.1"
                     /translation="MAPSRLRVFHGPEEPALAVESRVSIPLRATVTLPLGEVLPLLTD
                     AVKSRRQWVGDFYDDDITISSDLYEVLLAYQHFRRPSA"
     gene            1854..2168
                     /locus_tag="VFB80_20085"
     CDS             1854..2168
                     /locus_tag="VFB80_20085"
                     /inference="COORDINATES: protein motif:HMM:NF017958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HZN36146.1"
                     /translation="MTARREDPVLTSSRREAILVLLIWLVACTYSIGVCYAWGYGRDA
                     ATLTYIFGFPDWVFWGVIVPWSVCTLLALILGSFVVTDDDLGEEQAEVDLSPAPQEAD
                     RA"
     gene            2161..>3025
                     /locus_tag="VFB80_20090"
     CDS             2161..>3025
                     /locus_tag="VFB80_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN36147.1"
                     /translation="MLSTLLIAQATTTADKLPGWGAIIALGLFIAASVWLGMLAQRVV
                     ERSQFIQGYFQGNRGLGPWAIALTATVQSGGTFMGFPSLVYTYGWIVALWIGSYMVVP
                     ITGFALLGKRVAQISRRTGAVTVPDLFRARFASPTAGLVCSLLIIVFMTIMMLAQFKA
                     GATVMKEAWPLAVASSPAGATGPTWWPSQWPYLVGLVVFSLAVVGYTAIGGFLAAVWT
                     DLLQSVMMFAGVTVLACLVLTKAGGLEAGTKRAIEHAGPQFATGPGLKVQKDAEGNIQ
                     EIAFLPLTMAFS"
BASE COUNT          481 a          932 c         1035 g          577 t
ORIGIN      
        1 cactcctgcc aaagatcgtg tttcagcggc tatcgcgggt tacgtttggc ttgagcacgc
       61 aagtactttg tactgagtac cgagtaccag ctacacgcgg gctcccgctc aataccgatc
      121 atcgaacgac aatcgccgcc agtcaccata ggctagccct gttggttgcc aacgccgatt
      181 taatcgacgc ggccgcggtt gatcacgtcg atcagcagcc gcaggcggcc gaggttgcgg
      241 cggttgaagg cgaagaccag ccgcggcatg tgggccaggt cggcttcgtc gcgttccagg
      301 tccagggccg gacgttgcac gaaatggccc gcggtcaaga gatcgacgaa ctcgcggttg
      361 atcgcctcca agagctccgg ggagagcggc gtttgcagcc gcaggaccag gtcgctcttg
      421 acgtagcgca tgctgtgata gacgcggaag aactggagca cctcggcctt ggccgcttcg
      481 tggctgtcgg tcagcttgta gaggtggagg tcttccggcg agatcatgcg ggcccggagc
      541 aagtggttga tgacatactc gtgcagcgcc cgccagtagt ttccgccggg cgcgtcgagc
      601 agcacgacgg gaatcatgtc gcgcttgccg gtctgcagca gggtgatgac ctccatcgcc
      661 tcgtcgagcg tgccgaagcc gccgggcagg cagacgacgg cgtcgcattc cttcacgaac
      721 atcagcttcc gcgtgaagaa gtacttcatg tggacgagct tggtgtcgcc ctggatcacc
      781 gggttggccg actgctcgaa gggcagcatg atgttcaggc ccatcgaggc ttcgcggccc
      841 gcgccgcggt ggccggcctc catgatgccg ctggccgcgc cggtgatgac cagccagccg
      901 gcctgggcca tcgagcggcc gaaatccatc gcctgcacgt aggccggctc gtcgggctgg
      961 gtgcgggccg agccgaagac ggtgaccttg cgcttgcggc ggtacggggc gaagaccttg
     1021 aaggcgtacc gcagttcgcg aagcgtgcgg ctgaggattt tcaggtcgcc gcgggtcgag
     1081 ccatcgaggg ccagcttgtc ggccgtttcc ttgatcgtct gcaccaggtc ggcgatgtgc
     1141 gcccgcttgt cggctttttc gctgaccagg tcgaagctgg agatgtcgtc gtcggacgtg
     1201 cggaagaagg aatcgtcgtt cacaaggggc tcctcgaatc cagcgcctcc gcgccggcca
     1261 cctgccattc cattatacgt cgcggggtcg acgacattcg gaatcgccgg ccggcgtctc
     1321 gcgcagcaaa acagcccggc aaggccgggc tgtggcgtcg atcggaatga gcggctcggt
     1381 caggcgctgg gccggcggaa gtgttgatag gccagcagca cttcgtacag gtcgctgctg
     1441 atcgtgatgt cgtcgtcata gaagtcgccg acccattggc ggcggctctt caccgcgtcg
     1501 gtcagcagcg gcagcacttc gcccagcggc agcgtcacgg tcgcgcgtag cgggatcgaa
     1561 acccgcgatt ccaccgcgag ggccggctct tccggtccgt gaaagacgcg caaacggctg
     1621 ggggccatgc aggatccttc ctggcacgtg aatccgttca tgagcccgcg cgcatgcgtg
     1681 gcggcagcgc gaaagctcgt cgctaatccc gttatcggcc ggccgggggc cagaccttgg
     1741 gcaaacttcg agaaatggtg cggcgggctt tgccagccgc aagttgcggc ggctgctttc
     1801 gcggggttat attgcgatcc cgccttcccg cctcccaccc tgctagcttt gtgatgacgg
     1861 cacgccgaga agaccctgtg ctgaccagca gccggcggga ggcgattctg gtgctgctga
     1921 tctggctggt ggcgtgcact tattcgatcg gcgtctgcta tgcctggggc tacggccgcg
     1981 acgcggcgac gctgacgtat atcttcggtt ttccggattg ggttttctgg ggcgtgatcg
     2041 tgccctggtc cgtttgcacg ctcttggcat tgatcctggg cagctttgtc gtcaccgacg
     2101 acgacctggg cgaggagcaa gcggaagtcg acctgagccc agcgccgcag gaggccgacc
     2161 gtgcttagca cgctcctcat cgctcaggca accacaacag ctgacaagct gcccggctgg
     2221 ggcgcgatta tcgcgctggg cctgttcatc gcggcgtcgg tctggctggg catgctggcc
     2281 cagcgggtcg tcgagcggag ccagttcatt cagggttatt ttcaaggcaa ccgcggcctg
     2341 ggtccgtggg cgatcgccct gactgcgacc gtgcagagcg gcggcacctt catgggcttt
     2401 ccgtcgctcg tttataccta cggctggatc gtggccctct ggatcggcag ctacatggtc
     2461 gtgccgatca ccggctttgc cttgctcggc aagcgcgtcg cccagatctc gcggcggacc
     2521 ggagcggtga cggtgccgga cctgtttcgg gcccgcttcg ccagccccac cgccggcctg
     2581 gtctgctcgc tgctgatcat cgtgttcatg acgatcatga tgctggccca gttcaaagcc
     2641 ggcgcgacgg tgatgaagga ggcctggccg ctggcggtcg ccagcagccc ggccggagcc
     2701 accgggccaa cgtggtggcc aagccagtgg ccgtatctcg taggtctcgt cgtgttttcg
     2761 ctggccgtcg tcggctacac ggccatcggc ggcttcctgg cggccgtgtg gaccgacctc
     2821 ttgcagagcg tgatgatgtt cgcgggggtg acggtgctgg cgtgcctggt gctcacgaaa
     2881 gccggcggat tggaagccgg tacgaagcgg gcgattgagc acgccggccc gcagttcgca
     2941 accggtccgg gactcaaggt gcagaaggac gcggagggaa acatccagga aatcgccttc
     3001 ctgccgctga cgatggcgtt ctcga
//
LOCUS       DASRJB010000760         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1908956, whole genome shotgun sequence.
ACCESSION   DASRJB010000760 DASRJB010000000
VERSION     DASRJB010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908956"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1196
                     /locus_tag="VFB80_20095"
     CDS             138..1196
                     /locus_tag="VFB80_20095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36148.1"
                     /translation="MRRDFGTCLLAAAAGLFSAGCASTDERGPLSWARLPSPSATIRN
                     QPVSSSGPARSPARAAASDTRQSLWNRLAGGGKSAAAAKTAEHQQESLNLAILRGRNH
                     ERAGEWDKARALYEEARKQNPESPELAHRLGIVADAQRRHSEAEQLFQFALDRDRGNA
                     TVLSDLGYCYYLQGQLSKAESALLKATRLEPGNSRHWNNLALVIGHQGRYEEALECLR
                     ETGSEADAQYNLAFIYASQEKADQAKRCFQAALAADPLHRRAREALSSFEEYERLPVH
                     LREMDDLAENGVRYVPYVEGSEKGGVQQAAAEEGVATSMTATRAARALHMESQGLRSR
                     NMASQRSEDMAGQQSAAP"
     gene            complement(1200..1835)
                     /gene="ruvA"
                     /locus_tag="VFB80_20100"
     CDS             complement(1200..1835)
                     /gene="ruvA"
                     /locus_tag="VFB80_20100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZN36149.1"
                     /translation="MITKISGTLLRLDEESATLDCPPLEYQVYIAESSRRALQSLVGQ
                     PVSLHTIYYLDGDPSRGRVTPRLVGFRSEIEREFFEMFCSVDGVGAKKALRAMVRPVQ
                     DIAEMIEEQDAKGLSSLPGVGGATADRIIAKLRRKMSKFALLVAQSQDHAAEVERDVV
                     QEAFEALRALGHSDADARRLLDGVLRVKRKFPSVEAVLQTIYQQSHGREDA"
     gene            complement(1921..2238)
                     /locus_tag="VFB80_20105"
     CDS             complement(1921..2238)
                     /locus_tag="VFB80_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36150.1"
                     /translation="MNKTTLIRRWGIQNDNPTDDEMRSALGELDAIDPEHPDCWLSDQ
                     SGWSITAFASGLVTIENTESGEGPWHMHGQSKDEILRLWKHLQQGNLAAIQAQNWIDG
                     YNS"
     gene            complement(2307..2822)
                     /gene="ruvC"
                     /locus_tag="VFB80_20110"
     CDS             complement(2307..2822)
                     /gene="ruvC"
                     /locus_tag="VFB80_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZN36151.1"
                     /translation="MKRTTETATRILGVDPGLNITGYAVVERQLGRLNVIEAGMIRGR
                     SRGDLAARLAEIHEGIADVIASLKPEVLSLEELYSHYERPRTAILMGHARGVICLAAT
                     QAGLRVHSYSATQVKRLLTGNGRAPKSQVQRAICHEFALAAPPEPPDVADAIAIALCH
                     HYLQRDLAQSL"
     gene            complement(2819..>3604)
                     /locus_tag="VFB80_20115"
     CDS             complement(2819..>3604)
                     /locus_tag="VFB80_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZN36152.1"
                     /translation="AGKIGGAGEREKSAASVKESRSLGAGGLADIIAAQGGERIRFCL
                     LKTHYRSTVIYGDESLQEANENLERFYGYFERFLKVTRQRFFNLPAPAKREAGEFAPG
                     AEPLLQAVHRLRASFLEKMDDDFNTGGAIADLFDMVRELNRFANECNLDRFADVDSKD
                     TEKMKELFANPDVKLLVRATAVLRELSNILGLFRQTPSQRGGASEGVVPKLMMLLIEL
                     RAEARAKKDFATGDKIRKSLAEAGITLEDRKGGGTEWRIGGGS"
BASE COUNT          636 a         1163 c         1141 g          664 t
ORIGIN      
        1 ttccccgaag gctacaccgg cgtcgaatcc gaaggcagct actacaacgt catcggctcc
       61 gccttcggcg gcggcttcgg ccgccgcttc ggcggcgccg gcggcttcta tcagtaaacg
      121 cggacacggg ggataacatg cggagggatt tcgggacctg cctgctggcg gcagcggcag
      181 gcctgttcag cgcgggctgc gccagcacgg atgagcgcgg cccgctgtct tgggcccggc
      241 tgccttcgcc gtcggccacc atcagaaatc agccggtgag cagcagcggg ccggccaggt
      301 cccctgcacg ggccgctgcc tcggacacgc gccagtcgct ttggaaccgg ctggcgggtg
      361 gcggcaaatc ggcggcagca gccaagacag cggagcacca gcaggagtcg ctgaacctgg
      421 ccattcttcg cggccggaat cacgagcggg cgggcgaatg ggacaaggcc cgcgcgctgt
      481 atgaagaagc gcgtaagcag aatcccgaga gccccgaact cgcccatcgc ctgggcatcg
      541 tggccgacgc ccaacggcgg cacagcgaag ccgagcagct gtttcagttc gccttggatc
      601 gcgaccgcgg gaacgcaacc gtgctcagcg acctgggcta ctgctattac ttgcagggcc
      661 agctctccaa ggcagagagt gccttgctga aagcgacgcg gctggagccg ggaaactcgc
      721 ggcactggaa taacctcgct ttggtgatcg gacaccaggg gcgctacgag gaggccctgg
      781 aatgcttgcg cgaaacgggt tcggaggccg atgcccaata caacctggcg ttcatctatg
      841 ccagccagga aaaggcggac caggccaagc gctgtttcca ggcggccctg gccgccgatc
      901 cgctgcatcg ccgggcccgc gaagcgctct cgtcgttcga ggaatacgaa cggctgccgg
      961 tgcacctgcg cgagatggac gacctggccg aaaacggcgt tcgctatgtg ccctacgtcg
     1021 agggctcgga aaaaggcggc gtgcaacagg cggcggccga ggaaggcgtc gccaccagca
     1081 tgacagccac tcgggccgcc cgggccctgc acatggaatc gcagggcctc cgcagccgca
     1141 acatggccag ccagcggagc gaggacatgg ctgggcagca gtctgccgcc ccgtagcggc
     1201 tacgcgtcct cacggccgtg gctctgctga tagatggtct ggagcacggc ctcgacgctg
     1261 gggaactttc gtttgacgcg cagcacgccg tcgagcaggc ggcgggcatc ggcgtccgag
     1321 tggccaagcg cccgcagcgc ctcgaaggct tcctggacta cgtcgcgctc gacctcggcg
     1381 gcgtgatcct gcgactgggc gaccagcagc gcgaattttg acattttgcg ccgcagcttg
     1441 gcgatgatcc ggtcggcagt cgcgccgccg acgccgggca gcgacgagag ccctttggcg
     1501 tcctgctctt cgatcatctc ggcgatgtcc tgcaccgggc ggaccatcgc ccgcagggcc
     1561 ttcttcgcgc cgacgccatc gaccgagcag aacatttcga agaactcgcg ctcgatctcc
     1621 gagcgaaagc cgaccagccg gggcgtcacg cgtccgcgcg acggatctcc gtcgagatag
     1681 taaatcgtgt gcaggctgac cggctggccg acgagcgact gcagcgcgcg gcggctcgat
     1741 tcggcgatat agacctggta ctcgagcggc gggcaatcga gggttgccga ttcctcgtcg
     1801 aggcggagga gcgtcccgga gattttggtg atcacgtcag agttgtcgcc tgtctagctc
     1861 caatgaattt gaaccgcgga ttgcgcggat aacacggatg cgggcagttg ctcaattgtc
     1921 tcaagagttg tatccatcaa tccaattctg ggcttgaatc gcggcaaggt tcccctgttg
     1981 caaatgtttc cagagtcgca ggatttcatc tttgctttgg ccgtgcatgt gccaagggcc
     2041 ttccccagat tcggtgttct caattgtgac taacccactg gcgaatgcgg tgattgacca
     2101 accggattga tcggataacc agcagtcagg atgctctgga tcaatcgcat ccaactctcc
     2161 cagagcgcta cgcatttcgt cgtcggtcgg attgtcgttc tggattcccc accgccttat
     2221 gagagttgtc ttgttcactg gcttctccat acgcggcatt cttgtccgcg tcatccgcgc
     2281 aatccgcggt tctacttcct tcccgcctac agcgactgcg ccaggtcgcg ctggaggtaa
     2341 tggtggcaga gggcgatggc gatggcgtcg gcgacgtcgg gcggctccgg cggcgcggcg
     2401 agagcgaact cgtggcagat cgccctctgc acctggctct tgggagcccg gccgttgccg
     2461 gtcaacaggc gcttgacctg cgtcgcggag tagctgtgga cgcggaggcc ggcctgcgtg
     2521 gcggcgaggc aaatcacgcc gcgggcgtgg cccatcagga tcgctgtccg cggccgctcg
     2581 tagtgcgaat agagctcctc cagcgagagc acctccggct tcagcgaagc gatcacgtcg
     2641 gcgatgcctt cgtggatttc ggccagtcgc gcggccagat ccccgcgcga acggccgcgg
     2701 atcatgccgg cctcgatgac gttcaagcgg cccaactgcc gctcgacgac agcgtagccg
     2761 gtgatgttca agcccggatc gacgccgagg atgcgcgttg ccgtttcggt ggtgcgtttc
     2821 aagagccgcc cccgatccgc cactcggtac caccgccctt gcggtcttcg agcgtgatgc
     2881 cagcctcggc cagcgacttg cggatcttgt cgccagtggc gaagtccttc ttggcccgcg
     2941 cctcggcccg caactcgatg agcagcatca tcagtttcgg cacgacgccc tcgctcgctc
     3001 cgccgcgttg gctgggcgtc tgccggaaca agccaaggat gttcgacagt tcgcgaagca
     3061 cggccgtcgc ccttaccagc aacttcacgt ccggattcgc gaagagctcc ttcatcttct
     3121 ccgtatcttt ggaatccacg tcggcgaagc gatcgagatt gcactcgttg gcaaaccggt
     3181 tgagctcacg caccatgtcg aacaaatctg caatcgctcc gccggtatta aagtcgtcat
     3241 ccatcttctc caggaagctg gcccgcagcc gatgcaccgc ctgtagcaag ggctcggcac
     3301 cgggagcgaa ctcgcctgct tctcgtttcg cgggagcagg caggttaaag aatcgttgcc
     3361 gcgtgacttt caaaaacctt tcaaagtagc cgtagaacct ttcgaggttt tcattcgctt
     3421 cctgcagcga ttcatcgccg tagatgacag tgcttcggta gtgcgtcttc agcaggcaga
     3481 agcgaatgcg ctccccgcct tgagcagcaa tgatatctgc cagtccaccg gcacccagcg
     3541 accggctctc tttgacgctt gccgccgact tctcacgctc accggctccg cctatttttc
     3601 ccgc
//
LOCUS       DASRJB010000761         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_507750, whole genome shotgun sequence.
ACCESSION   DASRJB010000761 DASRJB010000000
VERSION     DASRJB010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507750"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1715
                     /locus_tag="VFB80_20120"
     CDS             111..1715
                     /locus_tag="VFB80_20120"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN36153.1"
                     /translation="MPTSARLSGRRRFDVVVIGGGITGLTAAWFLKRAGKKVGVLERD
                     RLASADTAHTTAHLTMVTDLRLKDLVKNLGKAAARLTWQGGVTAVNTIEKIADQLGID
                     CEFRRVPAFLMASLDSERDEREELQTEAELARELGFEADYLDAVPYFGRPGVRFPNQA
                     KFQPRRYLAGLAAAIKGDGSAIFEESEVTEVTAEKGEAFQVACGDAVALADYLVIATH
                     VPMMGVTGLVSATLLQTKIYPYSSYVVSGTLPHGVIPEACFWDTSDPYYYLRIEAGQT
                     TDGFIFGGLDHKTGQVADAAQRLDDLRAKVYSRIPEARIDHQWSGQIIESNDGLPLMG
                     ETAVRQFVATGFNGNGITFGTLGAMLACDAALGRDNPWQQLFSVDRKKLRGGTLNYVA
                     ENVDFPYYFLAGTLSPASRESPGSLQRGEGKVLSINGERAACSRDSGGQLHVVSAVCT
                     HLGCVVAWNDAEHTWDCPCHGSRFHATGEVLAGPAESPLEPVQWPTDEPAKPRSRRTP
                     RKKAAAAPKRRAAAKGATRSRRTSAR"
     gene            complement(1684..2190)
                     /locus_tag="VFB80_20125"
     CDS             complement(1684..2190)
                     /locus_tag="VFB80_20125"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HZN36154.1"
                     /translation="MLRWLELIFGGDVPQQPLELYQVAARAVLVYLVGLVVVRIGKSR
                     LVGRMTALDVIVGFILGSLLSRGITGQASISATAVASAAVVASHWALTWLTCQSHALG
                     VIFKGDAILLVENGQPLLEALRKTHISENELKEGLRLAGVDDIRQVRQAYRERNGEIS
                     VLKFDASG"
     gene            2445..>3840
                     /locus_tag="VFB80_20130"
     CDS             2445..>3840
                     /locus_tag="VFB80_20130"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN36155.1"
                     /translation="MTGPQFRRARRLIVENLEMRLALATFYVSPAGNDGQSGTSEAPW
                     HTLQAAADRVQSGDTVIVRSGNYVGFDLRRSGTPGNPIVFHAEPGATITQRSLRTPDG
                     INLEGADYVTVEGFTISGMPRAGIRAVENQSVTLRNNTLDLNARWGIFTGFSDDLLIE
                     VNVVSRTQIEHGIYVSNSGDRPVIRGNTLWGNRDNGIHMNGDATMGGDGIISGALVEA
                     NVLFDNSSGGGSAINADGVQNSTFRNNLIYNTHAAGISLYRMDGGGPSTGNIVSNNTV
                     LVAADGRWALQVHDGSTGNIVRNNVLHNDSSFRGSLSVSADSLPGFSSDYNALTGRFT
                     TSGGGSVLNLAQWRAATLSDAHSFIASPAELFVNPSGGDFHLPPGSPAIDAGTTLFAP
                     PTDLEGIVRPSGSGLDIGAFELPGVSPAGSPSALYHFDQSSGSAAADSSGNGQTATIV
                     NQPLLGQPGVTSAGS"
BASE COUNT          716 a         1293 c         1189 g          642 t
ORIGIN      
        1 acggcacggc gcctgcgccc actgctcccg agcggtagca gccaagcccc aactgccagg
       61 tgagtgacat gaagtcgatc cgttcgaatc cgatttggac tgagttccgc atgccgactt
      121 cggccaggct gtccggccgg cggcgcttcg acgtcgtcgt aattggcggc ggtatcacgg
      181 gcttgacagc cgcctggttc ctgaagcgcg ccggcaagaa ggtcggcgtg ctggagcgcg
      241 accggctggc cagcgccgat acggcgcata cgacggcgca cctgaccatg gtcaccgact
      301 tgcggctgaa ggacctggtc aagaatctcg gcaaggctgc ggctcgcctc acctggcagg
      361 gaggcgtgac ggcggtaaac acgatcgaaa aaatcgcaga tcagctgggc atcgactgcg
      421 aattccgccg tgtgccggcg ttcctaatgg catcgctcga cagcgagcga gacgagcgtg
      481 aagaattgca aaccgaagcc gagctggcgc gggagctggg ttttgaagct gactatctgg
      541 acgcggtccc gtatttcggc cggccgggcg ttcgctttcc gaaccaggcc aaatttcaac
      601 cgcgaaggta cctcgcgggg ctggccgccg cgatcaaggg ggacggctcg gcgatctttg
      661 aagaaagcga agtcaccgag gtcaccgcgg aaaaagggga agcctttcaa gtagcctgcg
      721 gcgacgccgt cgcactggcc gactatttag tgattgcgac ccacgtgccc atgatgggtg
      781 ttacgggact ggtctctgcg acgctgctgc agacgaagat ttatccctac tcgtcgtacg
      841 tcgttagcgg cacgctgccg catggcgtga ttcccgaagc ctgcttttgg gacacctccg
      901 atccctatta ctatttgcgg atcgaagccg gccagaccac cgacggcttc atcttcggtg
      961 gcctcgacca taagaccggg caggtggcgg acgccgcaca gcggctggac gacctgcggg
     1021 cgaaggtcta ttcgcggata cccgaggcca ggatcgacca ccaatggtcg ggccagatca
     1081 tcgagtcgaa cgatgggctg cctctcatgg gcgaaactgc cgtgcggcag ttcgtggcca
     1141 cgggcttcaa cggcaacggg atcaccttcg gcacgctggg ggcgatgctg gcctgcgatg
     1201 ccgccctggg gcgcgacaac ccctggcagc agttgttctc ggtcgatcgc aagaaactcc
     1261 gcggcggcac gctgaactac gtggcggaga atgtcgattt tccctattac ttcctcgcgg
     1321 gaacgcttag ccccgcctcg cgcgagtcgc ccggttccct ccagcgcgga gaagggaagg
     1381 tgctctcgat caacggcgag cgcgcggcct gctcgcgcga ttccggcggg cagctgcacg
     1441 tcgtctccgc ggtgtgtact catttaggct gtgtcgtggc gtggaacgac gccgagcata
     1501 cgtgggactg cccctgccat ggctcgcggt tccatgccac gggcgaggta ctcgccggcc
     1561 cggcagaatc gccgctggag cccgtgcagt ggcccacaga cgagccagcg aaaccgcggt
     1621 cccgccgcac gccgcgtaag aaagcagccg ctgcacccaa gcggcgagcc gccgccaaag
     1681 gcgctacccg ctcgcgtcga acttcagcac gctgatttcg ccgttccgct cgcggtaggc
     1741 ctgccggacc tgccggatgt cgtcgacacc cgccagccgc agcccctctt tcagctcgtt
     1801 ctccgagatg tgcgtcttcc gcagggcttc cagcagcggc tgcccgttct cgacgagcaa
     1861 tatggcgtcg cccttaaaga tcacccctaa cgcatgcgac tggcacgtca gccaggttag
     1921 cgcccagtgg ctggccacca cggcagccga cgccactgcg gtcgccgaga tcgacgcctg
     1981 cccggtgatc ccgcggctga gcagcgagcc cagaatgaag ccgacgatca cgtcaagcgc
     2041 cgtcattcgc cctaccagac ggctcttgcc gatccgcacg acgaccagac ccaccagata
     2101 gaccagcacg gcacgggcgg ccacctggta cagctccagc ggctgctgtg gaacgtctcc
     2161 tccaaaaatc agctccagcc accgcagcat tgcccagccc cttgtggaga aaacgcaaag
     2221 cacgtgcccg gggttggcag tttttaattc tttttaacct atttttaccg ggcgtttcgg
     2281 caacaaacct ttcttgccac cgctgtccaa tcgcgataag gtaccgagca cggaacattc
     2341 gacccattcg cgaggatgcg agagctaggt tgcggcggcg ggcattcgct cgccacagcc
     2401 gcaaatactg acggatcagt ttctcacgta agtcggaggc ggccatgaca ggcccccagt
     2461 ttcgccgtgc gcgccgtttg atcgtcgaaa acttggaaat gcggctggcg ctggcaacct
     2521 tctatgtctc gcccgccggc aacgacggac agagcggcac gtccgaggcc ccgtggcaca
     2581 cgctgcaagc ggcggccgat cgcgtgcagt ccggcgacac ggtcatcgtg cgaagcggca
     2641 actacgtcgg cttcgatctg cgacgaagcg gcacgcccgg aaatccaatt gtcttccacg
     2701 ccgagcccgg cgccaccatc acgcagcgca gcctgcggac gcccgacggc atcaacctgg
     2761 agggcgcgga ctacgttacc gtcgaaggct tcacgatcag cggcatgccg cgggcgggaa
     2821 ttcgggcggt cgagaaccag agcgtcacgc tccgcaacaa cacgctcgac ctgaacgccc
     2881 ggtggggcat cttcaccggc ttcagcgacg acctgctgat cgaggtcaac gtcgtctcgc
     2941 ggacgcagat cgagcacggc atctacgtct ccaacagcgg cgaccggccg gtcattcgcg
     3001 gcaacaccct ctggggcaat cgcgacaacg gcattcacat gaacggtgac gccacgatgg
     3061 gcggcgacgg catcatcagc ggcgcactgg tcgaggccaa tgtgctcttc gacaacagca
     3121 gcggcggcgg ttcggcgatc aacgccgacg gcgtgcagaa ctccacgttc cgcaacaacc
     3181 tgatctacaa cactcacgcc gccggcattt cgctctaccg gatggatggc ggcgggccat
     3241 cgacggggaa cattgtctcc aacaatacgg tgctcgtcgc cgccgacggc cgctgggccc
     3301 tgcaagtcca cgacggcagc accggcaaca tcgtccgcaa caacgtcttg cacaacgaca
     3361 gctcgtttcg cggcagcctg agcgtcagcg ccgacagcct gcccggcttt tcgagcgatt
     3421 acaacgcgct cacgggccgc ttcacgacca gcggcggcgg cagcgtgctg aacctggccc
     3481 agtggcgcgc tgccacgctt tcggacgcgc attcgttcat cgcctcgccg gctgaattat
     3541 tcgtgaatcc ctccggcggc gacttccact tgcccccagg cagtcccgcc atcgatgccg
     3601 gcacaacgct gttcgcaccg ccgaccgacc tggaaggaat cgtccgacca agcggcagcg
     3661 gcctcgacat cggcgcattc gagctgccag gggtctcgcc tgcgggcagc ccgtccgccc
     3721 tctatcactt cgatcagtcc agcggctccg cggccgcgga ttcctcgggc aacggccaga
     3781 cggcgacgat cgtcaatcag ccgctcttgg gccaaccggg cgtgacgtcg gccgggtcgt
//
LOCUS       DASRJB010000762         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2187481, whole genome shotgun sequence.
ACCESSION   DASRJB010000762 DASRJB010000000
VERSION     DASRJB010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187481"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2159)
                     /locus_tag="VFB80_20135"
     CDS             complement(153..2159)
                     /locus_tag="VFB80_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36156.1"
                     /translation="MAVDDTRLQSVFRRAAEAVDPASRAAILDLECAADAELRGRVEE
                     LLKDRDVATVRQHPAALHAGQSDASDGTVAIQDAIHEQTTVSSSAQSSALLADGMTLP
                     PAAGEDGPPALDFLEPSTKSGSLGRLGHYEVLEVLGQGGFGIVLRTFDEKLQRVVAIK
                     VLSPQLASTSPARKRFLREARAAARVRHENIVQIHAVEEQPLPYLVMEYIPGQTLQQK
                     LDEKGPLDVTEILTIGGQIARGLAAAHEQGLIHRDVKPGNILLESGVEPRVKITDFGL
                     ARTADDASMSQSGMISGTPLYMAPEQAQGESLDYRVDLFSLGSVLYAMASGRPPFRAG
                     TTLAVIKRVAEEDPRPIREIIPEVPEWLCNVIARLHVKDPALRWRSAAEVADLLGRHL
                     AHLRQPTLVPRPESVAVPKPANPRTAAAWAVAAAVLVALVAVATYPFWRPAPSIPPAP
                     AAGGGGVAALPPAPVLSSHELQGPAAVRDALLDFGEPERNFGGAERDNALRRGEQQVT
                     ALLVRFDLEKLDLSPQAAVAKATLSFYVWDPSSQGKTKVAAFPLLTAWEEDSATWRQP
                     GAGKSWRGESFALDADAGPAGPAVIVDPDQDGDTLDPPREYQLDVTELVRAWLSGKAA
                     NHGLAIAPVIDSSADAGVLSRFQVYGSEDWRVQFTPKLTVQSSP"
     gene            complement(2393..3139)
                     /locus_tag="VFB80_20140"
     CDS             complement(2393..3139)
                     /locus_tag="VFB80_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZN36157.1"
                     /translation="MSAPSESVQNRAQAPARSVWKWSVGGALVLLAAYVIAAYVVAPL
                     VWIGYAHRHPALDKIPGITYTGDDHPGDPINVALIGSEADLKKIMLAAKWYPADPLGL
                     RSDVKIAADTVLARPYDEAPVSSLYLWGRKQDLAFEQPVGDDPRQRHHVRFWRAADVD
                     QQGRPLWAGSATYDKRVGLSHTTGEITHHIAADVDAEREHLLGNLQQTGELAERFAID
                     GFHKTLEGRNGGGDPWHTDGRLGCGVIRSQ"
BASE COUNT          541 a         1138 c         1093 g          562 t
ORIGIN      
        1 gcctcgggca ggttgaagat caaccagccc tcgcccacgt cggtgaacga aaaccccagc
       61 ttgtcccgaa tgaacgctcg catttggtcg gcctgcgagc tgtagaacat cgtgtgcacg
      121 cctttgatca tgaagctctc ctgaagatgt ctttagggcg aggactgcac cgtcagcttg
      181 ggcgtgaatt ggacccgcca atcctccgag ccgtacacct gaaaccgcga gagaacgcct
      241 gcatcggcgc tcgagtcgat caccggggcg atcgccagcc cgtgattggc ggccttgccg
      301 ctcagccacg cccggaccag ctcggtcacg tcgagctgat actcgcgcgg cggatcgagc
      361 gtgtcgccgt cctgatcggg atccacgatc accgccggcc ccgccggtcc cgcgtccgcg
      421 tccagcgcga agctctcgcc ccgccacgac ttgcccgccc ccggctgccg ccaggtcgcc
      481 gagtcttctt cccaggcggt caacagcggg aaggcagcga ccttcgtctt gccctggctg
      541 ctgggatccc agacgtaaaa gctcagggtg gctttcgcga cggcggcctg cggcgacagg
      601 tccagcttct ccaggtcgaa gcggaccaag agcgcggtca cctgttgttc gccgcggcgc
      661 agcgcgttgt cccgctcggc gccgccgaag ttccgctccg gctcgccaaa gtcgagcagc
      721 gcgtcgcgga cggcggccgg cccctgcagc tcgtggctgc tcagcacggg cgcaggcggc
      781 aaggcggcca ctcccccgcc gcccgcggca ggcgcaggcg ggatggaagg cgcgggacgc
      841 cagaagggat aagtcgctac ggccacgagc gcgacgagca cggcagccgc gaccgcccaa
      901 gccgcggcgg tgcgcggatt ggccggcttg ggcacggcaa ccgactccgg ccgcggaacc
      961 agcgtgggct gccgcaaatg ggccaggtgc cggcccagca ggtcggccac ctcggccgcc
     1021 gaccgccagc gcagggcggg gtccttgacg tgcagccggg caatcacgtt gcacagccac
     1081 tcgggaacct cggggatgat ctcgcgaatc ggccgcgggt cctcctcggc gacgcgcttg
     1141 atcacggcca gcgtagtgcc agcgcgaaac ggcggccgcc cgctggccat cgcatagagc
     1201 acgctgccca ggctgaacag gtccacgcga tagtcgagcg actcgccctg ggcctgctcc
     1261 ggcgccatgt agagcggcgt gcccgagatc attccgctct gcgacatgct ggcatcgtcg
     1321 gcggtccggg ccaggccgaa atcggtgatc ttgaccctcg gctccacgcc ggactccagc
     1381 aggatattgc ccggtttcac gtcgcgatgg atcagcccct gctcgtgggc cgccgccagc
     1441 ccgcgggcga tctgcccgcc gatcgtcagg atctcggtca cgtccagcgg acccttttcg
     1501 tcgagcttct gctgcagggt ctggcccggg atgtactcca tcaccagata gggcagcggc
     1561 tgctcctcga cggcgtgaat ctgcacgatg ttctcgtgcc ggacgcgggc ggcggcccgg
     1621 gcctcgcgga ggaatcgctt gcgggccggc gaggtggaag cgagctgcgg cgagagtacc
     1681 ttgatggcca cgacgcgctg gagtttctcg tcgaacgtcc gcagcacaat cccaaagccg
     1741 ccctggccca gcacctccag cacctcgtag tggcccagcc ggcccagcga gcccgacttc
     1801 gtggacggtt cgaggaaatc gagagccggc gggccatcct cgccggctgc gggcggcagg
     1861 gtcattccgt cggccagcag ggcggaactc tgggcggagc tggagacggt cgtctgctcg
     1921 tggatagcgt cctgaatcgc caccgtgccg tcagatgcat cggactgtcc cgcatgcagc
     1981 gcggccggat gctgccggac ggtggccacg tcgcggtcct tgagcaactc ctcgacccgg
     2041 ccgcggagct ctgcgtccgc ggcacactcc aggtcgagaa ttgccgctcg gctggcggga
     2101 tcgacggctt cggccgcccg gcgaaagacc gactgcaagc gagtgtcatc aacggccatc
     2161 ggccaccttc tttcgccgaa cttgcaagcg tccggagact ggccagcgag gcgattttca
     2221 aacctccacg gggctgtatt ctcacgaatt cagcggagag aaaccagcta cagcccttca
     2281 gccagccact ttttggcgtc ttcgattctg gccgcgtcga tgctcgatca tcttgctgtc
     2341 ctccaatttg ccgatttcct tgaatgagac cctcgacctg acttgtgagc gtctactgcg
     2401 agcgaatcac gccgcagccg agtcgcccgt cggtatgcca cgggtcgccg ccgccgttgc
     2461 gtccctccag cgttttgtga aatccgtcga tggcaaagcg ctcggccagt tcgcccgtct
     2521 gctgcaagtt gcccagcaga tgctcgcgct cggcatcgac atccgccgcg atgtgatggg
     2581 taatctcgcc cgtggtgtgg ctaagtccga cgcgcttgtc gtacgtggca gagccggccc
     2641 acagcggccg gccctgctga tccacgtcgg ccgcgcgcca aaagcgaacg tgatgtcgct
     2701 gccggggatc gtcaccgacg ggctgctcga aggccaaatc ctgcttgcgg ccccagaggt
     2761 agaggctgct gacaggggcc tcgtcataag gccgggccag caccgtgtcg gccgcgatct
     2821 tcacatcgct gcgcaggccg agcgggtcgg ccgggtacca tttggccgcc agcatgatct
     2881 tcttcagatc tgcttcgctg ccgatgagag ccacgttgat cggatcgccc ggatggtcgt
     2941 cgcccgtgta ggtaatgccc ggaatcttat ccagcgcggg atgccggtgg gcgtaaccga
     3001 tccagaccag gggcgccacc acgtaagcgg cgatcacgta cgcggccagg aggacgagcg
     3061 ctccgccgac ggaccacttc catacgcttc tggcaggcgc ttgggccctg ttctggacgg
     3121 actcgctggg agcactcata cgcgacctgt caggcagagc ctttggggag cgggtcggcg
     3181 gctgctgcgc caaagggcga cagcagcgtc agcgctatag cccaaagcgc tgggcggatg
     3241 aagtagattc gcatgcgcga agtcctccgc gttacttgcc agactgattg cggatcgcct
     3301 cgtcgatctt cttcttcacg gcttccaggt tgaa
//
LOCUS       DASRJB010000763        11250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3324657, whole genome shotgun sequence.
ACCESSION   DASRJB010000763 DASRJB010000000
VERSION     DASRJB010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324657"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFB80_20145"
     CDS             <1..348
                     /locus_tag="VFB80_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36158.1"
                     /translation="PCSIESYVQKTTEAIMRIMRARRNSPAHVLTERSVCAHTRQFSA
                     AAHLSSQVAVPQQLTPKHAHPRVCALCAPRSDGVAVKPAGSAAMIHQIAKERHAAVRG
                     EVQPSRELYQLYI"
     gene            complement(750..1736)
                     /locus_tag="VFB80_20150"
     CDS             complement(750..1736)
                     /locus_tag="VFB80_20150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN36159.1"
                     /translation="MKYLVTGATGLLGNNIVRLLAATGEMVRVLARDESDPRALADLP
                     VERASGDVRDAAAVTAACKGVDVVIHSAGHVRIGWSQGEVQRQINVEGTRHVASAARA
                     AGARMVHVSSVDALGLGRRKQPGDEEKSFPGIVECPYVVTKREAERVVLEEVDRGLWA
                     SIVNPGIFFGPWDWKPSSGKMMLAVARYMLFAPTGAGSFCDVRDVAAGTIAAAARGKS
                     GRRYVLGGHNLSYRQGCHEMARVTSQRAPWIPMGPIFSAFAYPVLGVRSLLRRPEGEA
                     NAAMLAMSRQDHCFKSDRAQEELGYQIRPLRQTFEDTWQWFRDHGYAPNNGR"
     gene            1837..2958
                     /locus_tag="VFB80_20155"
     CDS             1837..2958
                     /locus_tag="VFB80_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZN36160.1"
                     /translation="MPKLLIKPVGTRAERKQFLELPWQINGRDPCWIPPLRQNQAELV
                     GYRHHPFYEDAEGQTFLALHDGRPVGRVLALINRAHNRVYKENRGFFGFFESIDDAEV
                     AAGLFEGVRWWLGERGITQFRGPMNPSMNYECGTLIEGFDTPPFFMMTHNPPYYQRLI
                     EGSGLAKVDDMFAFWGRIEMLQTLDKKLMFIYEECNRRFDIKLRRMDRKHFDRDVRIF
                     LSIYNQSLVGTWGFTPLSESEVEHMAGTLKLLIVPEMTTIAEIDGRPVGAQFGLLDYN
                     TRIKQIDGRLFPLGFIRLLWNKKGIKRIRLISTNVIPEYQKWGLGIVIAARLIPESQA
                     WGIEEAEFSWVLESNRLSFGTLQRGGAKITKRYRLFDST"
     gene            complement(3048..4136)
                     /gene="flhB"
                     /locus_tag="VFB80_20160"
     CDS             complement(3048..4136)
                     /gene="flhB"
                     /locus_tag="VFB80_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HZN36161.1"
                     /translation="MASEEFGDKTHEATPYRRQKAREEGQVVRSQDLASAALLIAGLL
                     VVWYFSYGLADVLGRLTEDQLGGEAWFSLDAQTTAHHAASVAARLAVSLLPLLGLLLV
                     AAVLGHMGQFGFLYLPQKLALDWQRLNPISNAQRMFSLTSFVHLGFGLVKVLLILGVA
                     AWCVWAERGRLQGLSGQTTAEIATYLLGVLLWTSLKIGGALLTLALFDYGYQWWQHEQ
                     DLRMTTQELKEEVKQQQGDPHIAQRRKQVARQMVLRRLSTAVPKADVIVTNPTELAIA
                     LQYDAEKMPAPVVVAKGAGVLAQRIRRLGLENNIPIVERKELARVLYANVEIGQAVPA
                     EQYAAVAEVIRYVYQLKRKPLPLARSGS"
     gene            complement(4207..4989)
                     /locus_tag="VFB80_20165"
     CDS             complement(4207..4989)
                     /locus_tag="VFB80_20165"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZN36162.1"
                     /translation="MSPLLIHYLDQFLIFVLVLTRLGCLLMTLPVVGTSSVPWQVRAI
                     LAIGVSLLLTPLYFGRAVPLPGNLLMLGVLVAREAIVGLALGLAVMILLSGMQLAGQI
                     ISQISGLSLADVVNPTFETNVPIFSHVLELLALAIFFLVGGHRDVLDALLRSFAWMPP
                     GEARLPSELVPALSQITAHSFEVGIRASAPVMVALLIATLIVALISRTLPQLNAVAVG
                     LNFNSLIVLGVLGVCLGSAAWVFQEELGSTIDFVGQAFLPAS"
     gene            complement(5057..5332)
                     /gene="fliQ"
                     /locus_tag="VFB80_20170"
     CDS             complement(5057..5332)
                     /gene="fliQ"
                     /locus_tag="VFB80_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HZN36163.1"
                     /translation="MDPQTAVDLTQQAMLTALIVSAPLLLVGMAVGLLIGLAQALTQV
                     QDQTVAFVPKIVAMAAILVFCLPWLVQKMVEYSEYLISNIPSTIAGG"
     gene            complement(5398..6237)
                     /gene="fliP"
                     /locus_tag="VFB80_20175"
     CDS             complement(5398..6237)
                     /gene="fliP"
                     /locus_tag="VFB80_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651465.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZN36164.1"
                     /translation="MIRALIAALVVALAPGVLRAQSSSTAPQAAPAPPAAPILPDPIT
                     EALRGGPESWTKPDKLSSTLQVMLLLTVLSLAPALLLMTTSFVRIVVVLGLLRQAIGA
                     QQLPPSQVITTLALFMTLLVMGPTWAEVYSRSILPYTRGEITDPQVAWDAGALPIKRF
                     MSRQIDLAGNSDDVWLFYEYLPESQRSSKPPETYDDVPLVVLLPAFMLSELKVAFLIG
                     FQIYLPFLILDLVVSSVTISMGMMMLPPVMISLPLKLLLFVLVDGWTLVVRMLLESFG
                     APL"
     gene            complement(6234..6851)
                     /locus_tag="VFB80_20180"
     CDS             complement(6234..6851)
                     /locus_tag="VFB80_20180"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZN36165.1"
                     /translation="MRFCASSLLVAALLAPAAAAAQSPASYYLRPAPRGEQEHSTRQA
                     APAPPALKLAPRSAASRSGVARPAAPTPGGAIGTVAGSLGIVLGLFFVIAWCSRRFVP
                     ADQRQLPKEAVEMLGRAPFAGKQQLQLLRVGNKLLLVAHSASGVETLTEITDAAEVEH
                     LTALCRRGQPGSSSAAFRQVLNQIAAEPAPGGFIGQTPRTARGAR"
     gene            complement(6882..7469)
                     /gene="fliN"
                     /locus_tag="VFB80_20185"
     CDS             complement(6882..7469)
                     /gene="fliN"
                     /locus_tag="VFB80_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZN36166.1"
                     /translation="MPGDAPTLKQDDIEELLRQAQMASGLLTDAPPAASAAAGADPAM
                     ASPTATFDAPQPMPRSRPAPGEPMGDDVEFLLAQAEQAIASVDRPVDPAIAGLAPFQL
                     NDLGGAPASGERATLELLKDVDLDLRIELGRTQMYLEDVLKLKRGSVVTLDKLAGDPV
                     DVFVNGRMVARGEVLVLNDNFCVRVTELVTGDEFD"
     gene            complement(7599..8117)
                     /locus_tag="VFB80_20190"
     CDS             complement(7599..8117)
                     /locus_tag="VFB80_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZN36167.1"
                     /translation="MNKTIIVAALVTVVIVGEALFAYLLIPSTGDLEKWAAAKNAQSR
                     HGDGNAHAGEHGSSGQAAPSGSHEVEVDLGKYNVIVHQPTANVTLRVSFHLIGTVAEK
                     EEAEFQHLLEKNQHRLRDQAIFEIRNCQIEDLTDPGLALLKRRILAKSNDLFGKPLLH
                     SVVFSDFSFIEQ"
     gene            8564..10399
                     /locus_tag="VFB80_20195"
     CDS             8564..10399
                     /locus_tag="VFB80_20195"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HZN36168.1"
                     /translation="MSLDLRRRLLAELDQLVLIDPHTHINPLDPGSHTLADILGYHYY
                     TELAHSAGMPKAQIEEPGLDPREKVRRLIENLGPIQNTIQYSWLVEMCQTFFGLHDPD
                     ITPANWEKVYDSAERLMCHADWPQQVLERSKLEAVFLTNDFDDPLEGFDTSVYIPCLR
                     TDDLVFHLAKPDVFQRLELCSGIGISDAKALRRAIAALFQHFKSRGARACAISLPPDF
                     APARVSPARANTALAAIHSEGAGAAESHRRAIGNFVFWTLAEMCDAFKLPFDLMIGVN
                     RGVYEAGVHQGRDLFDSRVSLIQYKELFNSFPGVTFPISVLASVTNQELVSYAWIFPN
                     VACNGHWWYSNTPAYIEHDLAARLEAVPRTKQVAYYSDMYKLEFALPKFAMFKRILAK
                     VLAERFVIDRGWTETQAVALGKQVLRGNVETMFRWGQDLREELPDEALLEKMPAPALL
                     AFGPITAPSEGSEGGLDELGFHTQSNLPKEEVLRSKDDEVVIPVPPADASDDFSFELT
                     PREAPAEKEPPALEVVADAGEVTDELDLDVAPTEEGDSGGIHEDVLTPLDEVADVPQA
                     GQAEEEFDAVLLEPEAEESDVPTSESSRPTEPFPIKPSSKKPGGK"
     gene            complement(10528..>11250)
                     /locus_tag="VFB80_20200"
     CDS             complement(10528..>11250)
                     /locus_tag="VFB80_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36169.1"
                     /translation="LDSPRYAVRRRLLPGADDAKAWRHVAFSSDGQLLAAGNAQGTWS
                     VVDPQSWTALLQEKLPAAVTALAWHPTDRRRLAIATEDGQLTLVPLPADVTSRKAAPD
                     FDPANLLATAKTVVTNEEWSDLVRLLNVVAAYPLSVAHKQEFDNLRFKAKKAIGDLVS
                     AVQPKTIDAVDVEDAAYNLQLAIDFDPAGPLGKDAREKLRQLPVQTPESIAKANEAAQ
                     KAAAAGKNRPRPGARPKVRAKP"
BASE COUNT         2039 a         3685 c         3606 g         1920 t
ORIGIN      
        1 ccttgctcca tcgagagtta tgtccaaaaa acgaccgaag cgattatgcg cattatgcgc
       61 gcccgccgta actctccagc acacgtgcta accgagcgat ccgtatgcgc gcatactcgg
      121 cagttttctg ctgcagcaca tctttcgtcg caagttgctg tgccgcaaca acttacgcca
      181 aaacatgcgc acccgcgagt atgcgcatta tgcgcgccgc gcagcgatgg cgtcgcggtc
      241 aagccggcag ggagcgcggc gatgatccac caaattgcca aggaacgaca cgctgccgtc
      301 cgtggcgagg tccagccaag ccgcgaattg tatcaactgt acatataaag tcaagaagcg
      361 caaaagggta gtgggtcagt ttgaattggg agtttcaccg caagcttctc gaaagcacgc
      421 ccagcagacc ggcacttgat cgtcgatccc gacgaatacc cgctctagcc catagtcgcc
      481 gtaaaacgtt gttttcggtt ccgaatatcc caaccgtgtc gcacaattcg ggcatagccg
      541 aacagcccac actacaccta gttcatcgtc cgcagcttcg acaatggcgg gctcgtctat
      601 gcgtatgccg gctaggattt gtttctccaa gtgcgagcaa acgagcggcg caggcgctcc
      661 gccgtgcttg tcacatatga cgtatggcat ggaactagcc ccacactctg cttgcccaat
      721 cctaggtgcg ggtgcctaag ggagccattt caccgcccgt tgtttggcgc gtagccgtgg
      781 tcgcggaacc attgccaggt gtcctcgaac gtctgccgca gggggcggat ctggtagccg
      841 agctcttcct gggcccggtc gctcttgaag cagtggtcct gccggctcat ggcgagcatg
      901 gccgcgttgg cttcgccttc gggccgccgc agcaggctgc gcacgccaag caccggatag
      961 gcaaaggcgc tgaaaatcgg ccccatcggg atccaaggcg cgcgctgcga cgtcacgcgg
     1021 gccatctcgt ggcagccttg gcgatagctc agattgtggc cccccagaac gtaccgccgg
     1081 ccgctcttgc cgcgagctgc cgcggcgatc gtgccggcgg ccacgtcgcg gacgtcgcaa
     1141 aagctgcccg caccggtcgg cgcgaacagc atgtaccgcg cgacggccag catcatcttg
     1201 cccgacgacg gcttccagtc ccacggcccg aagaaaattc ccggattgac gatcgacgcc
     1261 cacaatccgc ggtcaacttc ctccagcacg acccgttcgg cctcgcgttt ggtcactaca
     1321 tacgggcatt ccacgatgcc cggaaaactc ttttcttcat ccccgggctg cttgcggcgg
     1381 ccgaggccca gcgcatcgac gctcgagacg tgcaccatcc gcgcaccggc cgcccgcgca
     1441 gccgaggcga cgtgccgcgt gccttcgacg ttgatctgcc gctgaacctc cccttgcgac
     1501 cagccgatcc gcacgtgccc cgccgagtga atgaccacat cgacgccctt gcaggccgcg
     1561 gtcaccgcag ccgcgtcgcg cacgtcgccg gaagcccgct cgaccggcaa atcggccagc
     1621 gcacgcggat cgctttcgtc gcgagccagc acccgcacca tttctcccgt cgcggccagc
     1681 agccggacga tattgttccc cagcagtcct gtggccccgg ttaccaggta cttcatagcc
     1741 aatcccgcgg ctggtggaat tcgattatca tcgcgagttt atcaaaaatc cttggtccgc
     1801 agcgatggca gcggccgcta caggataacg ctttgcatgc ccaaattgct gatcaagccc
     1861 gtcggaacga gggccgagcg caagcagttt ctggaattgc cttggcaaat taacggccgc
     1921 gacccctgct ggattccccc gctgcggcaa aaccaggccg agctcgtcgg ctaccggcat
     1981 cacccgttct acgaagatgc ggaggggcag acctttctgg ccctccacga tggccggccc
     2041 gtcgggcgcg tgctcgcgct catcaatcgg gcccacaacc gggtgtacaa ggagaaccgc
     2101 ggcttcttcg gcttcttcga gtcgatcgac gacgccgaag tcgccgcggg cctgttcgag
     2161 ggcgttcgct ggtggctggg cgagcgcggc atcactcagt ttcgcggccc gatgaacccg
     2221 tcgatgaact acgagtgcgg gacgctgatc gagggcttcg acacgccgcc cttcttcatg
     2281 atgacgcaca acccgcccta ctatcagcga ttgatcgagg ggagcgggtt ggcgaaagtc
     2341 gacgacatgt tcgcgttctg gggccgcatc gagatgctgc agacgctgga taaaaagctg
     2401 atgttcatct acgaggaatg caaccgccgg ttcgacatca agctccgccg gatggaccgc
     2461 aagcacttcg accgcgacgt gcggatcttc ctgagcatct acaaccagtc gctggtcggc
     2521 acttggggct tcacgccgct ctcggaatcc gaggtcgagc acatggccgg cacgctgaag
     2581 ctgctgatcg tgcccgagat gaccacgatc gccgagatcg acggccggcc ggtcggggct
     2641 cagttcggcc tgctcgacta caacacccgc atcaagcaaa tcgacggccg attgttcccg
     2701 ctgggattca ttcgcctgct ctggaacaag aaggggatca agcggatccg gctgatcagc
     2761 accaacgtga tccccgagta tcagaagtgg ggcctgggaa tcgtcatcgc cgcccggctg
     2821 atccccgagt cccaggcttg gggcatcgaa gaggcggagt tcagctgggt cctggaaagc
     2881 aaccgcctct cgttcggtac gctgcagcgc ggcggcgcga aaatcaccaa gcggtatcgg
     2941 ctgttcgact ccacttagcc tcggcttcct aatcttgatc ctaatcgtaa tcctaatcct
     3001 cgggccggag agcacgatta ggattaggat taggattagg attgcgatta ggagccggag
     3061 cgcgccagcg gcagcggctt ccgttttagc tgatagacgt agcggatgac ctcggcgacg
     3121 gcggcgtact gctcggcggg gacggcctgg ccgatctcga cgttggcgta gaggacgcgg
     3181 gccagctcct tccgctcgac gatcgggatg ttgttctcca ggccgaggcg gcgaatccgc
     3241 tgggccagga ctccggcgcc cttggccacg acgaccggcg cgggcatttt ctccgcgtcg
     3301 tattgcaggg cgatcgccag ctcggtcggg ttggtgacga tcacgtcggc cttgggcacc
     3361 gcggtgctca gccggcggag gaccatttgc cgggcgacct gcttgcggcg ctgggcgatg
     3421 tgcgggtcgc cttgctgctg cttgacttct tccttgagct cctgcgtcgt catgcgcaag
     3481 tcctgctcgt gctgccacca ctgatagccg tagtcgaaca gagccagcgt caagagtgcg
     3541 ccgccgatct tgaggctcgt ccagaggagc acgcccagca ggtacgtggc gatttcggcc
     3601 gtcgtctggc cggacaggcc ttgcagcctg ccgcgctccg cccagacgca ccaggcggcg
     3661 acgcccagga tcagcagcac tttgaccagc ccaaagccca aatgcacgaa gctcgtcagc
     3721 gaaaacatcc gctgggcatt gctgatggga ttcagccgct gccaatcgag cgccagcttt
     3781 tgcggcaggt acagaaagcc gaactggccc atgtgcccca gcaccgcggc aactagcagc
     3841 aggcccaaga gtggcagcag gctgaccgcc agtcgggcag cgacgctggc ggcgtgatgt
     3901 gccgtggtct gggcgtcgag gctgaaccag gcttcgccgc cgagctgatc ctccgtaagg
     3961 cggcccagga cgtcggccag accgtaggag aagtaccaga ccacgagcaa gccggcgatc
     4021 aagagcgccg ccgacgccag gtcctggctg cgcacgacct gcccttcttc gcgggctttc
     4081 tgccggcgat agggcgttgc ctcgtgcgtc ttgtcgccaa attcttcgga ggccattcag
     4141 ctcacaaagc cgcgaccagt ggtcgcgctc tgccgattgc accgcgacca ctggtcgcgg
     4201 ctttggttac gaggcaggca ggaacgcctg ccccacgaag tcgatcgttg agcccagctc
     4261 ttcctgaaag acccacgcgg ccgagcccag gcagacgcca agcacgccca gcacgatcag
     4321 cgagttgaag ttcaggccga ctgcgaccgc gttgagttgc ggcagcgtgc ggctgatcag
     4381 cgccacgatg agcgttgcga tgagcagcgc taccatgacc ggcgcagagg cccgaatgcc
     4441 gacttcgaaa ctgtgagccg tgatctgcga cagcgccggc accagctcac tgggcagtcg
     4501 ggcctcgccg gggggcatcc aggcaaacga tcgcagcagc gcgtcgagca cgtcgcggtg
     4561 gccgccgacg agaaagaaaa tcgccagggc caggagctcc aatacgtggg agaagatcgg
     4621 cacgttcgtt tcgaacgtcg gattgacgac gtcggccagc gacaggccgc tgatctggct
     4681 gatgatctgc ccggcgagct gcatgcccga aagcaggatc atcacggcca ggccgagcgc
     4741 gagccccacg atcgcctcgc gagccacgag cacgccgagc atcagcagat tgcccggcag
     4801 cgggaccgcg cggccgaaat agagcggcgt gagcagcagc gagacgccga tcgccaggat
     4861 cgcccggact tgccagggca cgctcgacgt gcccaccacc ggcagcgtca tcagcaggca
     4921 gccgaggcgc gtcaggacca gcacgaagat caggaactgg tcgagatagt gaatcagtaa
     4981 gggagacatt gcaccgcaca gtttcgtcat tggtcactgg tcattggtca tttgcaaatg
     5041 acgagtgaca aatgactcac ccaccggcaa tcgtgctggg aatgttggag atcaagtact
     5101 ccgaatactc gaccatcttc tgcacgagcc aggggaggca gaacacgagg atggcggcca
     5161 tggcgacgat cttggggacg aaggcgacgg tctggtcctg cacttgcgtg agggcctggg
     5221 ccaggccgat caagaggccg accgccatgc cgaccagcag caggggcgcg ctaacgatca
     5281 acgccgtgag catcgcttgc tgcgtcaggt cgacggcggt ttgcggatcc atgttcaaag
     5341 ccgcgaccac tggtcgcggt ctactgattg caccgcgacc actggtcgcg gctttggtta
     5401 caggggcgct ccaaagctct ccagcagcat tcgcacgacc agcgtccagc cgtcaacgag
     5461 cacaaacagc agcagcttga gcggcagcga gatcatcacc ggcggcagca tcatcatgcc
     5521 catcgagatc gtcacgctgg agacaaccag gtcgaggatc agaaacggca ggtaaatctg
     5581 aaagccgatc aggaacgcga ccttgagctc gctgagcatg aaggccggca gcagcacgac
     5641 cagcggcacg tcgtcgtacg tttccggcgg cttgctgctc cgctggctct cgggcaggta
     5701 ctcgtagaac agccagacgt cgtcgctgtt gccggccagg tcgatctgcc ggctcatgaa
     5761 ccgcttgatc ggcagcgcgc cggcgtccca ggccacttgc gggtcggtga tctcgccgcg
     5821 ggtatagggc agaatgctgc ggctgtagac ttcggcccag gtcgggccca tcaccagcag
     5881 cgtcatgaac agggcgagcg tcgtgatgac ctggctcggc ggcaactgct gcgccccgat
     5941 cgcctggcga agtaagccca gcacgacgac gatccgcacg aagctggtcg tcatcaagag
     6001 cagggccggc gcgaggctca gcacggtcaa cagcagcatg acctggagcg tcgagctgag
     6061 cttgtccggt ttcgtccacg actccggtcc gccgcggagg gcttcggtga tcgggtcggg
     6121 caggatcggc gcggccggtg gagcgggcgc ggcctgcggc gcagtggacg atgactgagc
     6181 gcgcaagaca cccggtgcga gcgcgaccac taacgcagcg atgagagctc ggatcatcgt
     6241 gcgcctcgcg cggtacgtgg cgtctggccg atgaatccgc cgggggcggg ctcggcggcg
     6301 atctggttca ggacctgccg aaacgcggca gacgagctgc ccggctggcc gcggcggcag
     6361 agggccgtga ggtgctcgac ttccgccgca tcagtgattt ccgtcaacgt ctcgactccg
     6421 ctcgccgagt gggcgacgag cagcagcttg ttgccgacgc gcaggagttg gagctgctgc
     6481 ttgccggcaa atggggcgcg gccgagcatt tccacggctt ccttgggcaa ctggcgctgg
     6541 tccgccggca cgaagcgccg cgagcaccag gcgatcacga aaaacaggcc gagcacgatg
     6601 cccaggctgc ccgcgaccgt gccgatcgcc ccgccgggcg tgggtgcagc cggacgggcg
     6661 acgccggatc ggctggccgc actccgcggc gccagcttga gtgccggcgg cgcgggtgcc
     6721 gcctggcgcg tggaatgttc ctgctcgccg cgcggggcgg gccgcaaata gtacgacgca
     6781 ggcgactgcg ccgctgcagc agccggcgca agcagcgcgg cgaccagcag cgatgacgca
     6841 caaaagcgca tgcgaatgac tcgcagaaca gggccgccgg actagtcgaa ttcgtcgccc
     6901 gtgaccagct cggtcacgcg gacgcaaaaa ttgtcgttaa gcacgagcac ttcgccgcgg
     6961 gccaccatcc ggccgttgac gaacacatcg actggatcgc cggccagctt gtcgagcgtg
     7021 accacgctgc cccgcttcag cttcagcacg tcttccaggt acatctgcgt gcggcccagc
     7081 tcgatccgca gatcgagatc gacgtccttg agcagctcca gcgtcgcccg ctcgcccgac
     7141 gccggcgctc cgccgaggtc gttcagctgg aacggcgcga gccccgcaat cgccggatcg
     7201 accggccgat cgaccgaggc gatcgcctgc tcggcctggg ccagcaggaa ttccacgtcg
     7261 tcgcccatcg gttcgccagg ggccggccgc gagcgcggca tcggctgggg cgcgtcaaac
     7321 gtcgcagtcg gcgaggccat cgcgggatca gcccccgccg cggccgaagc agccggcggc
     7381 gcatcggtca gcagccccga ggccatctgg gcctggcgga gcagctcctc gatgtcgtcc
     7441 tgcttgagtg tcggcgcgtc cccaggcatc ggattccttt ccgatcgcaa gtcgttcggc
     7501 agggaaaagg ggcaaccgcg gattacgcgg atttcacgga tgaggaaaat cgctttcctg
     7561 tcttcatccg cgctatccgc gacatccgcg gttccccgtc actgctcgat gaacgagaaa
     7621 tcgctgaata cgaccgagtg cagcagcggc ttgccaaaca ggtcgttgct tttcgccaaa
     7681 atgcgccgtt tgagcaaggc caatcccgga tcggtcaggt cctcgatctg gcagttgcgg
     7741 atctcaaaaa tcgcctggtc ccgcagccgg tgctggttct tctccagcaa gtgctggaac
     7801 tcggcttcct ccttctccgc caccgtgccg atcaggtgga agctgacccg cagggtgacg
     7861 ttggccgtcg gctgatggac gatgacgttg tatttgccca ggtccacttc gacttcgtgg
     7921 ctgccggagg gcgcagcctg cccggaactc ccgtgctcgc cagcgtgggc attcccgtca
     7981 ccatgcctgg actgggcatt cttcgcagcc gcccattttt ccaggtcgcc cgtgctgggg
     8041 atgagcaggt aggcgaacag ggcctcgccc acgatcacca ccgtcacgag ggcggccaca
     8101 atgatggtct tgttcatcgc acgggtcctg ggtttccggt tcctcgaaaa cctacctccc
     8161 aggatgggac gcggagccgg cctgcgcagc acgactggct cataatgcca tcccccggcc
     8221 gaagggctcg cacatcgctt ttttgtcaag atctcgtcgc aaactctggc ggctcatcga
     8281 cttgcgccca gcatcgcgaa acggccttaa gtagttgtta ttagcgtttg agggcctccc
     8341 agccgagaga aacactcgcc gctgctcgcg cgcgctcccg gccaaaaagg cagtccgcgc
     8401 cgcattcgga acgtaacagc aaatagcgta ccaatgtaca caattccgtc gagacgcatc
     8461 ccggccagtc ccgcgcggcg ggcggctgaa ggctgcagca tcgaccgcgc gggcgtgcta
     8521 tccttgtggg cggttttccc ccgccccttt tctccccgcg actatgtccc tcgacctgcg
     8581 ccgccggctg ctggccgaac tcgaccagct ggtgctcatc gatccgcaca cgcacatcaa
     8641 cccgctcgat cccggctcgc acacgctggc cgacatcctc ggctatcact actacaccga
     8701 gctggcccac tcggccggca tgcccaaggc ccagattgaa gagcccggcc tcgatcctcg
     8761 ggaaaaggtc cgccggctga tcgagaacct ggggccgatc cagaacacga tccagtacag
     8821 ctggctggtc gagatgtgcc agacgttttt cggcctgcac gaccccgaca tcacgccggc
     8881 caactgggag aaggtctacg actcggccga gcggctgatg tgccacgccg actggccgca
     8941 gcaggtcctc gagcggagca agctcgaagc ggtgtttctg accaatgatt tcgacgaccc
     9001 gctcgaaggt tttgacacca gcgtctatat cccctgcctg cggaccgacg acctggtgtt
     9061 tcacctggcc aaaccggacg tgttccagcg gctggaactg tgcagcggga ttggcatcag
     9121 cgacgccaag gccctgcggc gggcgatcgc tgcgctcttt cagcacttca agtcccgtgg
     9181 ggcgcgggcc tgcgcgattt cgctgccgcc cgattttgcc cccgcgcggg tttcgccggc
     9241 gcgggccaac acggcgctgg ctgcgatcca ctccgaaggt gccggcgctg ccgaatcgca
     9301 ccgccgggcg atcggcaact tcgtcttctg gacgctggcc gagatgtgcg acgcgttcaa
     9361 gctgcccttc gacctgatga tcggcgtcaa ccgcggcgtc tatgaagccg gcgtgcacca
     9421 gggccgcgac ctgttcgaca gccgcgtctc gctgattcag tacaaggagc tgttcaacag
     9481 cttcccgggc gtgacgtttc cgatttcagt gctggccagc gtgacgaacc aggagctggt
     9541 gagctacgcc tggatctttc cgaacgtcgc ctgcaatggc cactggtggt acagcaacac
     9601 gccggcctac atcgagcacg acctcgccgc ccggctggag gcggtcccgc ggaccaagca
     9661 ggtcgcctac tacagcgaca tgtacaagct ggagttcgcc ctgccgaaat tcgcgatgtt
     9721 caagcggatc ctggccaagg tgctcgccga gcggtttgtc atcgaccgcg gctggaccga
     9781 gacgcaggcc gtcgccttgg gcaagcaggt gctccgcggc aacgtcgaga cgatgttccg
     9841 ctgggggcag gacctgcgcg aggaattgcc cgacgaagcg ctgctggaaa agatgccggc
     9901 tcccgccctg ctggcgtttg ggccgatcac cgctccgtcg gaagggagcg aaggcgggct
     9961 ggacgaattg ggtttccata cgcagtcgaa cctgcccaag gaggaggtgc tccgctccaa
    10021 ggacgacgag gttgtgatcc cggtcccgcc ggccgatgcg agcgacgatt tctcgtttga
    10081 gctgaccccg cgcgaggcgc ccgccgagaa ggagccgccg gccctggagg tcgtggccga
    10141 cgcgggcgaa gtcacggacg agctggacct cgacgtcgcg ccgaccgaag aaggcgattc
    10201 cgggggcatt catgaagacg tgctgacacc gctcgatgaa gtcgccgacg tcccccaggc
    10261 cggccaggcc gaagaagaat tcgacgccgt gctgctcgag cccgaggcgg aagagagcga
    10321 cgtgcccacc agcgaatcgt cccgcccgac cgagccgttt cccatcaagc cctcgtccaa
    10381 gaagcccggc ggcaagtagg cgcaggttgt agggtgggtc gaggctgccg cagccgatga
    10441 cccacgcgtt tctggcggat gatgcgtttg ccgcgtgggt ctgcgctcgc ggactcgctt
    10501 gacccaccct acaaccactg aattcggtca cggcttcgcg cggaccttgg gtcgcgcgcc
    10561 gggccgcggg cggtttttcc cggcggcggc cgccttttgc gccgcctcgt tggccttggc
    10621 gatcgattcg ggcgtctgca ccggcaattg ccgcagcttt tcgcgagcgt ccttgcccag
    10681 cgggcccgcg ggatcgaagt cgatggccag ctggaggttg taggccgcgt cttcgacgtc
    10741 aacggcgtcg atcgtcttgg gctggacggc gctgaccagg tcgccgatgg cctttttggc
    10801 cttgaagcgg aggttgtcga actcctgctt gtgcgccacg gacagcggat acgccgcgac
    10861 gacattcagc aggcgcacga gatccgacca ctcttcgttg gtcaccactg tcttggccgt
    10921 ggccagcaga ttggccgggt cgaagtcggg agcggccttg cggctggtga cgtcggcggg
    10981 cagcggcacg agggtcaact gtccgtcctc ggtggcgatg gccaggcggc ggcggtcggt
    11041 cggatgccag gccagggccg tgacagccgc gggcagcttc tcctggagga gcgcggtcca
    11101 ggattgcgga tcgacgaccg accacgtgcc ttgagcgttg ccggcggcca gcagctgccc
    11161 gtcgctggag aacgcgacgt gccgccaggc cttggcgtcg tcggcgcccg gcagcaggcg
    11221 gcggcgcact gcgtacctcg gcgaatccag
//
LOCUS       DASRJB010000764         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2477783, whole genome shotgun sequence.
ACCESSION   DASRJB010000764 DASRJB010000000
VERSION     DASRJB010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477783"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFB80_20205"
     CDS             <1..306
                     /locus_tag="VFB80_20205"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN36170.1"
                     /translation="GGRLPLSEALSARARRLAEAAVSALPPATGYVGVDVILGAAGNG
                     DRVIEINPRLTTSYVGLRAFAKCNLAAAMLAVAEGREPDLSFGGGRVEFLADGTIVK"
     gene            326..1339
                     /locus_tag="VFB80_20210"
     CDS             326..1339
                     /locus_tag="VFB80_20210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZN36171.1"
                     /translation="MKWLALDVGGANIKAADGQGYAQSYAFALWRDSSRLAQQIRTSI
                     SEAPPADHLAVTMTGELADCFESKAAGVRFILQAISAGSDNRHTRVYLVDGRLVTPQV
                     ALTMPQLVAASNWHALARFAARYAPAGSGLAIDVGSTTCDVIPLVDGKPVAKGTTDTQ
                     RLLAGELVYSGVERSPVCGVTAQVPYRGQTCPVVHEMFATMRDVYLLLDQLPEDPALT
                     ATADGRPATKAAARQRLARMIAADGDEFNHRDAVTLAESVADAQVSRLAAAIGKVKGS
                     LPAAPQTIVLSGHGEFLARESLDLLQIAAACVSLTKELGPAISRSAPAHALAVLARES
                     TGK"
     gene            1336..1914
                     /locus_tag="VFB80_20215"
     CDS             1336..1914
                     /locus_tag="VFB80_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36172.1"
                     /translation="MNPPRLPIRVVKVGGSLLDWPKLPAALREWLAAQPPAVQVLICG
                     GGPLTDVIRQADRAFSLGEEAAHWLCIDALAVSARLLSALLAGVPLIESLGELPASGP
                     AVFDPRRFLIEEEPRLPGCVLPHTWAATTDSIAARVTEVMEAPEVVLLKSADPPTAAS
                     MKGLAAAGYVDAHFPSAAAAIAEVRFVNLRRF"
     gene            complement(2001..>3307)
                     /locus_tag="VFB80_20220"
     CDS             complement(2001..>3307)
                     /locus_tag="VFB80_20220"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HZN36173.1"
                     /translation="IAGLLLAADVGFAQCGFGRFPSVGGISINPEGVLSEPPADAVPE
                     LVRQELKNAKPVAGALSEKVTIRKISLKAIEAALAKSEQNNAAELPQEIKFLAGIQRI
                     QYVLVYPDDIVLAGPGEGWKLDSRGNYVGVTTGRPVVRLEDLVVALRTVNDAREGAIS
                     VSIDPTEEGRKRFESYMKSQKTFTPAVLDGIAQALGNQTIKIRGVPETSRFARMLAAS
                     DYKMKRIAMKLEESPLPELPSFLDMMKKGGAKLGNMMPRWWMACNYEPLAKSEDGLAW
                     EIRGQGVKVLTEDDYVAGGKATATGKVNPLAKEWADTMTKHYDKLAVKEPVFGDLRNL
                     MDLCVVAALIQKEGLQQKANLQLPTLQGQDSKFELVYWHAPKTVATQCSFVKRGSEYI
                     ITASGGVEIASWEVAGKSILNAEVGQVRSKATPQTAGSLWWN"
BASE COUNT          496 a         1114 c         1093 g          604 t
ORIGIN      
        1 ggcggccggc tgccgctgtc ggaggccctg tcggcccgcg cccgccgcct ggcggaagcg
       61 gcggtcagcg cgctgccgcc ggcgaccggc tacgtgggcg tggatgtgat cctgggagcg
      121 gctggcaatg gtgaccgcgt aatcgaaatc aacccgcggc tgaccacttc gtatgtcggc
      181 ctgcgggcgt ttgccaaatg caacctggcc gccgcgatgc tcgccgtcgc cgaaggccgc
      241 gaaccggatt tgagcttcgg cggcgggcga gtagaattct tggcggacgg caccattgtg
      301 aagtagcggc cccgcggaac gcaccatgaa atggctggcc ttggatgtcg gtggggcgaa
      361 tatcaaagcg gccgacgggc aaggctatgc ccagtcgtac gcctttgcct tgtggcgcga
      421 ttcgtcgcgg ctggcccagc agattcgcac gtcgatctcg gaagcgccgc cggccgatca
      481 cctggcggtc acgatgacgg gcgagctggc tgactgcttt gaatcgaagg cggccggcgt
      541 gcggttcatc ctccaggcga tcagcgccgg ttcggacaat cgccatacgc gggtgtacct
      601 ggtcgatggc cgcctggtga cgccgcaggt cgctcttacg atgccgcagc tcgtggcggc
      661 gtcgaactgg catgcactcg cccgctttgc cgcgcggtac gcgccggcgg gaagcgggct
      721 ggcgatcgat gtgggcagca cgacctgcga cgtgatcccg ctggtcgatg gcaagccagt
      781 cgccaaaggt accaccgaca cgcagcggct gctggcaggc gagctggttt atagcggcgt
      841 ggagcggagc cccgtctgcg gcgtgacggc gcaggttccc taccgcgggc aaacttgccc
      901 cgtggtgcac gagatgttcg ccacgatgcg cgatgtctac ctcctgctcg accagctgcc
      961 ggaagacccg gcgctcaccg cgacggccga cggccggccg gcgaccaagg ccgcggcccg
     1021 ccagcggctg gcgcggatga tcgccgccga cggcgacgag ttcaatcatc gcgacgcggt
     1081 gacgttggct gaaagcgtcg ccgacgcgca ggtctcgcgc ctggcggctg cgatcggcaa
     1141 agtgaagggc tctttgcccg cggcgccgca gacgatcgtg ctctccggcc acggggagtt
     1201 tctggcccgc gagtctctcg atttgctgca aatcgcggcc gcgtgcgttt cgctgacgaa
     1261 ggagttgggg ccggccatct cgcggagcgc cccggcccat gcgctggccg tgctggcccg
     1321 cgaaagcacc ggcaaatgaa cccgccgcgg ctgccgattc gcgtcgtcaa ggtcggcggc
     1381 agcctgctcg attggccgaa gctgccggca gcgctgcgtg aatggctcgc cgcccagccg
     1441 ccggccgttc aggtgctgat ttgcggcggc ggaccgctga ccgacgtcat tcggcaagcg
     1501 gaccgcgcgt tttccctcgg cgaggaagcc gcgcattggc tgtgcatcga cgcgctggcc
     1561 gtttcggcac ggctgctctc ggcgctgctc gccggcgtac cgctgatcga gtcgctggga
     1621 gaattgccgg cgagcgggcc agccgtgttt gatccgcgcc ggttcttgat cgaagaggag
     1681 ccgcgactgc cgggctgcgt tcttccgcac acttgggccg cgacgacaga ttcgatcgcg
     1741 gcccgcgtga cggaggtaat ggaagcgccc gaagtcgtac tgctgaaatc ggcagacccg
     1801 ccgacagcgg cttcgatgaa gggcctcgct gccgcgggct acgtcgatgc ccattttccc
     1861 agtgctgctg cggcgattgc cgaagtccgg ttcgtcaacc tgcgtcgatt ttagcgcctg
     1921 ctcctaacga aaacggcccg gctgaattgc acacacccgg gccaggagag gcttagttcg
     1981 tcgcttgttc ggcggccgtc tcagttccac cacaggctgc ccgcggtctg cggagtagcc
     2041 ttgctgcgga cctggccgac ctcggcgttc aggatcgact tgccggcgac ttcccacgag
     2101 gcgatctcga cgccgcccga ggcggtgatg atgtactcgc tgccgcgctt cacaaagctg
     2161 cactgcgtgg cgacagtctt gggagcgtgc cagtaaacca gctcgaactt gctgtcctgg
     2221 ccttgcagcg tgggcagctg caggttggcc ttctgctgca ggccttcctt ctggatcagc
     2281 gccgccacga cgcacaggtc catcaggttc cgcaggtcgc caaacaccgg ctctttcacc
     2341 gcgagcttgt cgtaatgctt cgtcatcgta tcggcccact ccttggccag cggattgacc
     2401 ttgccggtcg ccgtcgcctt gccgcctgcg acgtagtcgt cctcggtgag gaccttcacg
     2461 ccctggccgc gaatctccca cgccaggccg tcttcgctct tggcgagcgg ctcatagttg
     2521 caggccatcc accaccgcgg catcatgttg ccgagcttcg ccccgccctt cttcatcatg
     2581 tccaaaaagc tgggcagctc cggcagcggc gactcttcca gcttcatcgc gatccgcttc
     2641 atcttgtagt cgctggccgc gagcatgcgg gcaaaccggc tggtctccgg gacgcccctg
     2701 atcttgatcg tctgattgcc gagcgcctgg gcgattccgt ccagcaccgc cggcgtgaac
     2761 gtcttctgcg acttcatgta ggactcgaac cgcttgcggc cttcctcggt cggatcgatc
     2821 gagacgctaa tcgcaccttc gcgggcatcg ttgacggtcc gcagcgcgac cacgagatcc
     2881 tctagccgca ccaccggccg gccggtcgtc acgcccacgt agttgccgcg gctgtcgagc
     2941 ttccagcctt cgcccggccc ggccagcacg atgtcgtcgg gatagacgag gacgtactga
     3001 atccgctgaa tgcccgcgag gaacttgatc tcctgcggca attctgcagc gttgttctgt
     3061 tcgctcttgg ccagggcggc ttcaatcgcc ttcagcgaaa tcttgcggat cgtgaccttt
     3121 tcgctgagcg cgccggcgac gggcttggcg ttcttcagct cctgtcgcac gagttccggc
     3181 accgcgtccg cgggcggctc gctcaacacg ccctcgggat tgatcgagat gccgcccacc
     3241 gacgggaacc ggccaaagcc gcattgggca aagcccacgt cggcagccag cagcaggccc
     3301 gcaatcg
//
LOCUS       DASRJB010000765         9681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4170160, whole genome shotgun sequence.
ACCESSION   DASRJB010000765 DASRJB010000000
VERSION     DASRJB010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4170160"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFB80_20225"
     CDS             <1..336
                     /locus_tag="VFB80_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HZN36174.1"
                     /translation="YSVISPEGCAGILWKSHQYAERAATALKFTSADLLRLKVVDHVI
                     EEPLGGAHRDHHSTAMRVKHYLMRSLRELLALSTDQLLAGRYEKFRRMGVFLEDPAAV
                     AKANGHDPS"
     gene            complement(314..574)
                     /locus_tag="VFB80_20230"
     CDS             complement(314..574)
                     /locus_tag="VFB80_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36175.1"
                     /translation="MTRSAEEWIKLLDERPFTPLRLLLADGRVHEIRHRQLAIVSQDS
                     VTIGIPRNRQSKIAKKITRCSIPNIIAIEPLETDGPKKGRGR"
     gene            complement(882..1577)
                     /locus_tag="VFB80_20235"
     CDS             complement(882..1577)
                     /locus_tag="VFB80_20235"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZN36176.1"
                     /translation="MQPYDILMLIVLAGAVVWGAWKGLAWQIASLASIALSYFVALRF
                     RGQLAGVISAAPPWNVFLSMLILFLGTGLVVWVGFNFVSELIEKVKLKEFDRQLGALF
                     GAAKGVLLCVLITLFSVALLSDTQRQAICNSRSGYYIALLLDRSDAIIPQELHQVLEP
                     YLDQLDRSVPHEHVETGRLGSFSFGSTEPSPPGSPPSSFPAAEFTPLVPVQTPRSAGR
                     IEAQPAQPAGSFR"
     gene            1737..2492
                     /locus_tag="VFB80_20240"
     CDS             1737..2492
                     /locus_tag="VFB80_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN36177.1"
                     /translation="MAGFQTHITVSTITGIAYGVWGFQCGAPVETCVLAGGLCSVSGM
                     LPDLDSDSGRPVHEMSAFAAAIVPMLMLERFENFGWSRESMVLAGAAIYAAIRFGVAE
                     LFKRYTVHRGMWHSIPACLACGLLAFLVVAGQDIAIRAFKAGAVSLGFLSHLLLDELW
                     SLRLRSGKLNVKRSFGTALKFFGTDKWANLSVYGTLAVIALLAVGDPMLMNRFGYEVK
                     FGPHTAQQVLDKALELAGVQKQPAAGEATTIQR"
     gene            complement(2607..3056)
                     /gene="dtd"
                     /locus_tag="VFB80_20245"
     CDS             complement(2607..3056)
                     /gene="dtd"
                     /locus_tag="VFB80_20245"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZN36178.1"
                     /translation="MKAVLQRVSRASVTVEGQVVGQIGRGLLVLLGVEQDDALADAQQ
                     LADKTVQLRIFDDADGKMNLALADVGGALLVVSQFTLLGDCRKGRRPSFIQAAPPALA
                     EQLYENFVAAVGVQGIPVATGKFRAMMQVELVNDGPVTLLLDSRKRY"
     gene            complement(3053..3727)
                     /locus_tag="VFB80_20250"
     CDS             complement(3053..3727)
                     /locus_tag="VFB80_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36179.1"
                     /translation="MVRIFAVLAVLAVLLLAANFAIGLAAGDFNSVALQKKAAQIRVL
                     ELENQARRNRTRSSPELKTARQALSEADAAFAGPRRWMTIHMLLGSAAALVAVLVNSI
                     TVTYFIGTSRWCKEVCQTYRLDAELAEASTRLKRGTFPWALAGILAIIAIVGLGAAAD
                     PSGANWRSSASLVLPHYVAAIVGLAVVVVSFWMQANRIAENHRVIEAILAAVRTVRGE
                     KEPLPT"
     gene            complement(3736..4758)
                     /gene="ftsY"
                     /locus_tag="VFB80_20255"
     CDS             complement(3736..4758)
                     /gene="ftsY"
                     /locus_tag="VFB80_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZN36180.1"
                     /translation="MGVFDVFKKKTEQPATTAGQGSAPAESPQEPAPKQGLFGMLRSA
                     LKKTSDLLNTDVRDLFKREGRLVDDEFLGELFAILVKTDMGAGPAARIRDRIGTDFRA
                     RIVHMEDVLKSVKDEVRIILAQPERPIELAATGPTVILVVGVNGSGKTTSIAKLANLF
                     RSQGKKVVLGAGDTFRAAAVEQLTIWADRLGVEIVKGGAGGDPAAVAHQAVAKALESQ
                     AEICIIDTAGRLQTQTNLMQQLEKIHRVIGKQIPAAPHETLLVLDATAGQNALSQAQG
                     FSTAAKCTGIVLAKLDGTAKGGVIIPIRERFAIPVKFVGLGEKASDLAVFNADQFVEA
                     LFAGAA"
     gene            complement(4856..5260)
                     /gene="nusB"
                     /locus_tag="VFB80_20260"
     CDS             complement(4856..5260)
                     /gene="nusB"
                     /locus_tag="VFB80_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZN36181.1"
                     /translation="MSRRHRAREVVLQVLYEDDLNPGRNPEAADQFLCNRLNNDAESI
                     DFARSLLAGVRKHRAEIDQLLAQRADNWTPDRMAVTDRNILRLGAYEILYGGTPERVA
                     VNEAVELAKRYGAKQSPHFVNGVLDRFMHKER"
     gene            complement(5410..6444)
                     /gene="ruvB"
                     /locus_tag="VFB80_20265"
                     /pseudo
     CDS             complement(5410..6444)
                     /gene="ruvB"
                     /locus_tag="VFB80_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
     gene            6636..8720
                     /locus_tag="VFB80_20270"
     CDS             6636..8720
                     /locus_tag="VFB80_20270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN36182.1"
                     /translation="MASFAPGPLVDPVRALGGCPSDEQLLALIPGDLAPELASSVTAH
                     LETCVSCQSRLTTLRHRSRTAPLEPTTPPPRLDRRPSDPPAAGPDAPTIYLPQPPLRV
                     PERIGQYKLLKLIGRGGMGLVYLAQHVKLGRTVAIKLLPGLQLSDKSAIVRLQREIAA
                     AGRVQHPNIVFATDANEEDGIDYLVMEYIDGVDIGRLVAVLGPLPCPAACEIIRQAAL
                     GLAHIHACGLVHRDLKPSNLMLASDGVVKILDLGLARLREGQPADDDSQTQTGYLLGT
                     ADYVSPEQLHDPHDADVRSDLYSLGCTFFKLLAGQAPYSGTDGGSIGKKLDAHRYAAI
                     PSIRSLRPDVPEEVDAVLSRLLAKEPGDRIQQPSELAELLAPLSAGSDLCGLYRPGRE
                     QAEIDEPPPPLPLQTPLPDGGSTAVKGRTPTPTRLLAAEPPRKKTWTWISTAAVALCL
                     LAAAGTTGWAIAHLTGLTGIQRKDQPIHGRGPQTFDLSRPLEEIAWEGYESSPPARYN
                     DGQRMLELRSESYQLIKLGDYDGRPGTFEVTIRQTPWHGFAGLFFGYRVQPRRDVPQL
                     ASTFQLVWLHHFPLKDGNGRKYGEQMEAMRQRTVLGDDTQPFIREAAIGQRVPLPARA
                     QVRLKVQFGRQGCEKISVDDVVVEDLTAANINQKYDAIDYQGPWGLFSARGYGPKEAT
                     WFGNLSFTPLEN"
     gene            8724..9572
                     /locus_tag="VFB80_20275"
     CDS             8724..9572
                     /locus_tag="VFB80_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36183.1"
                     /translation="MSCMIDYPTAGGEGAVDAGGFLVSCCGQWLRPNTWDPVAPCRIV
                     AKVYAAGAAVDAGVPNDGTEGTLLPDNMASGGDFHFNISLIPDATVGGAHQIKVWLQN
                     LDTGQFNADGPIDFNGCEFGVGGCADPPCLGQVETVPCELAIDQPAPQEYRVGPDLRA
                     TSFIGEALDAWKQGELKFAGIKLEYSFPLSSPSRAVWTTGGDSRALRLEVVRAASGFV
                     ATLYLLQASNRGVEPPRRWRSKDFNLNRGGVLRALTACCKETDATIYVSVPDGKKHCE
                     PERTVV"
BASE COUNT         1682 a         3160 c         3091 g         1748 t
ORIGIN      
        1 tactcggtga tcagccccga aggctgcgcc ggcatcctct ggaagagcca tcagtacgcc
       61 gagcgggccg cgaccgcgct caagttcacc tcggcggacc tgttgcggct gaaggtcgtc
      121 gatcacgtca tcgaggagcc gctgggtggc gctcaccgcg accaccactc gacggcgatg
      181 cgggtcaagc actacctgat gcgttcgctc cgcgagctct tggcgctatc gacggaccag
      241 ttgctcgccg gccgctacga gaagttccgc cggatgggcg tcttcctcga agatccggcc
      301 gccgtggcca aagctaacgg ccacgaccct tcttaggccc gtcggtctcg agcggctcga
      361 tcgcgatgat gttggggatc gagcaacgcg tgatcttctt cgcgatcttg gactggcggt
      421 tgcgcggaat gccgatcgtg accgagtctt gagagacgat cgccaactgc cggtgacgaa
      481 tctcatgcac tcggccgtcg gccagaagca atcgcagcgg cgtgaaggga cgctcgtcca
      541 gcagctttat ccattcttcg gctgaacggg tcattggacg ttttttggtg ctgtcagcaa
      601 aattatcacg ccggcaacga cgagcagccc gcctccccac aaacagccgg ccacacgttg
      661 tctagcactg gcgattcttt cggccgtcca ttcgtcgcat agcgaaagcc cagaaaggct
      721 ggcagcgccc cgattccggt tacgatccag ccaataacca gtcgtgtaag tcttggtgtc
      781 atcggtcgaa ttctaatcgg acaacgtccg ataatgtttc ttatgttcgg ttccaggccc
      841 gtttttccct gggttttcag cagccgcgag tccctgcggc tctagcgaaa gctgccggcg
      901 ggctgcgcgg gctgtgcttc gatgcggccg gcgctgcgcg gcgtttgcac gggcacgagc
      961 ggcgtgaact cggcagccgg gaagctggac ggcgggctgc cgggcgggct cggttcggtt
     1021 gagccgaacg agaacgatcc cagccggccg gtttcgacgt gctcgtgagg caccgagcgg
     1081 tcgagctggt ccaggtacgg ctcgagcacc tggtgcagct cctgcgggat gatcgcatcg
     1141 gaacggtcca ggagcagcgc gatgtaataa ccggaacggg agttacagat ggcctgccgc
     1201 tgggtgtcgg agagcagcgc gacggagaac agcgtgatca gcacgcacag cagcacgccc
     1261 ttggccgcgc cgaacagcgc gccgagctgc cggtcgaact ccttgagctt gaccttctcg
     1321 atcagctccg aaacgaagtt gaagccgacc cagacgacga ggccggtgcc aaggaacagg
     1381 atcagcatcg agaggaagac gttccaaggc ggcgcggcgc tgatcacgcc cgcgagctgc
     1441 ccgcggaagc ggagcgccac gaagtagctc agcgcgatcg aggccagcga agcgatctgc
     1501 caggccagcc ccttccacgc gccccagacc acggcgccag cgagcacgat cagcatcagg
     1561 atgtcgtaag gctgcatttg cgcgcgctcc ttcgcatttg gcaaacgggg cggagtatag
     1621 ccgccgcgga ggattggccg aatagcaacc caactgccat tggccaaggc gccggctctt
     1681 gctatcgtcc ccgccccgca ttgtctcgcg gggcagggaa aggcagggga ctggccatgg
     1741 ccggctttca gacgcatatc accgtgagca cgatcacggg catcgcctat ggcgtgtggg
     1801 gcttccagtg cggcgcgccg gtcgagacct gcgtgctggc gggcgggctg tgcagcgtct
     1861 ccggcatgtt gccggacctc gacagcgact ccggacggcc ggtgcacgag atgagcgcct
     1921 tcgccgcagc gatcgtgccg atgctgatgc tcgagcggtt cgagaacttt ggctggtcgc
     1981 gggagagcat ggtgctggcc ggggcggcca tctatgcggc gatccgcttc ggcgtcgccg
     2041 agctgttcaa gcgctacacg gtccatcgcg gaatgtggca cagcatcccg gcttgcctcg
     2101 cctgcggcct gttggcgttt ctggtcgtgg ccgggcagga catcgcgatc cgcgccttca
     2161 aggccggcgc ggtctcgctc ggcttcctct cgcacttgct gctcgacgaa ctctggagcc
     2221 tgcggctgcg gagcggcaag ctcaacgtca aacgctcttt cggcaccgcg ctcaaattct
     2281 tcgggaccga caagtgggcc aatctttcgg tctatggcac gctggctgta atcgccctcc
     2341 tggccgtcgg cgacccgatg ctcatgaacc gttttggcta cgaagtcaaa ttcggcccgc
     2401 acaccgccca gcaggtgctc gacaaggccc tggagctggc gggcgtgcag aagcagccgg
     2461 cggcgggtga agcgacgacg atccagcggt agaaacaaga accgaagaac caaagaaccg
     2521 aagaaccaaa gaaccaaaga accaaagaac caaaacagca cgacttccga tttggaattt
     2581 tggttcttcg gttcttgaga tttgtctcag taccgcttcc ggctgtccag cagcagcgtc
     2641 accggtccat cgttgaccag ctcgacctgc atcatggccc ggaactttcc cgtcgcgacc
     2701 gggatgccct gcacgccgac ggcggcgacg aagttctcgt agagctgctc ggcgagcgca
     2761 ggcggggcgg cctggatgaa gctcggccgg cggcccttgc ggcaatcgcc cagcagcgtg
     2821 aactggctga ccacgagcag cgccccgccc acgtctgcca gcgccagatt cattttgccg
     2881 tcggcgtcgt cgaagatgcg gagctggacc gtcttgtcgg ccagctgctg cgcatcggcc
     2941 agcgcgtcgt cctgctccac gcccagcagg accaggaggc cgcggccgat ctggcccacg
     3001 acctggccct ctacggtaac gctcgcccgg ctgactcgct gtaagacggc tttcatgtcg
     3061 gtagaggttc cttctcgccg cgaaccgtcc gaacggccgc gaggattgcc tcgattacgc
     3121 gatggttctc ggcgatccga ttggcctgca tccagaacga gacgacgacg accgccaaac
     3181 cgacgattgc agcgacgtag tgcggcagca cgagcgacgc cgagctgcgc caattcgcgc
     3241 cgctgggatc ggcggccgcg cccaggccga cgatcgcaat gatcgccagg atgccggcca
     3301 gcgcccaggg aaacgtgccg cgcttcaggc gcgtactcgc ttccgcgagc tcggcgtcca
     3361 gtctgtacgt ctggcagacc tccttgcacc agcggctggt gccgatgaag taggtcacgg
     3421 tgatgctgtt gacgagcacc gccaccagcg cggcggcgct gcccaggagc atgtggatcg
     3481 tcatccagcg ccgcgggccg gcgaaggcgg catcggcctc ggacagcgcc tgcctggccg
     3541 ttttgagctc gggcgacgag cgcgttcggt tgcgtcgcgc ctggttctcc aattcgagca
     3601 cgcggatttg ggccgccttc ttttgcaaag ccacggagtt gaaatccccc gcggccagac
     3661 cgatggcgaa attcgccgcc aggagcagca cggccaatac agcgaggaca gcaaagatgc
     3721 gaaccaaggg gcaacctacg ccgcgccggc gaacaaggcc tcgacgaact gatcggcgtt
     3781 gaacacggcc aggtcgctgg ccttctcgcc gaggccgacg aacttgaccg ggatggcaaa
     3841 ccgctcgcga atcgggatga tcacgccgcc cttggcggtg ccgtcgagct tggccaatac
     3901 gatgccggtg cacttggcgg ccgtcgaaaa gccctgggcc tggctgaggg cgttctggcc
     3961 ggccgtcgcg tcgagcacca gcagcgtttc gtgcggcgcc gccggaatct gcttgccgat
     4021 cacgcggtga atcttctcca gctgctgcat caggttcgtt tgcgtctgca gccggccggc
     4081 ggtgtcgatg atgcaaatct cggcttgcga ttcgagcgcc ttggctaccg cctgatgggc
     4141 aaccgccgct gggtcccccc ctgccccgcc cttgacgatc tccacgccca gccgatcggc
     4201 ccagatggtg agctgctcga cggccgcggc gcggaacgtg tcgcccgcgc cgaggacgac
     4261 cttctttccc tgcgagcgga acaggttcgc cagcttggcg attgaagtcg tcttgccgct
     4321 gccgttgacg ccgaccacca ggatcacggt cggcccggtg gccgccaatt cgatcggccg
     4381 ctcgggctgg gcaagaatga tgcggacttc gtccttgacg ctcttgagca cgtcttccat
     4441 gtgcacgatg cgagcgcgaa agtcggtccc gatccggtcg cgaattcgcg ccgccggccc
     4501 cgcgcccatg tcggtcttca ccaggatcgc aaacagctcg ccgaggaact cgtcatcgac
     4561 cagccggcct tcccgcttga agagatcgcg gacgtcggtg ttgagcaggt ccgaggtctt
     4621 cttcagcgcc gagcggagca tgccgaacag gccctgcttc ggcgcaggct cctgcggcga
     4681 ttccgccggc gcactcccct gccctgccgt cgtcgccggc tgctcggttt ttttcttgaa
     4741 tacgtcgaag acgcccatgg gattcgaaat ccaaagaacc aaagaaccga agaaccaaag
     4801 tcgaacatgt cctatttggt tctttggatc ttcggttcct tgatttttgg actcgttacc
     4861 tttccttatg cataaaccgg tccagcacgc cgttgacgaa atgtggcgac tgcttcgcgc
     4921 catagcgctt ggctaattcc acggcttcgt tgaccgccac ccgctcgggc gtgccgccgt
     4981 agaggatttc gtacgcgccc agccgcagga tgttgcggtc ggtcaccgcc atccgatcgg
     5041 gcgtccagtt gtcggcccgc tgggcgagca gctggtcgat ctcggcccgg tgcttgcgga
     5101 cgccggccag tagcgagcgg gcgaagtcga tcgactccgc gtcgttgttc agccggttgc
     5161 acaagaactg atccgccgcc tccgggttac gtcccggatt cagatcgtcc tcgtacagaa
     5221 cctgaagcac gacttcccgt gcccgatggc ggcgcgacat atcaggatta tagcttgcaa
     5281 gcagaaaccg gagagtggac caattattcg ccgggaacaa acctggtcct ggggcggcag
     5341 gaattgttcc gactcgcaca gggcactcgc agcactccgg gagcaggttt gctcccggct
     5401 aagccagtgc taggcaaaca gctttccctg cgcttcgtcc ggcctggccg gtgctgcgat
     5461 cttcaggtgc tcgtaggcct tcgccgtcgc cacgcggccg cgtggcgagc ggatcaatag
     5521 ctcgctgcgg agcaggtaag gctccacctc gtcagacagc gtgtcgggcg agaggttcat
     5581 cgtgtgggcg attgcttcga cccctgccgg cccgccgccg aaaacgcgga tgatcgtctc
     5641 caggcaagcg cggtcctgtt tgtccagtcc gaactcgtcg atgttcgcca tcaccaaggc
     5701 cgcgcgggcg acctccagcg tcaccacgcc gcccgacttg ctgtcggcat agtcacggac
     5761 ccaccgcagg cggtgattgg cgatccgtgg cgtatcgcgg ctgcggcggg cgatctcgat
     5821 cgccgcctct tcgtgcaact gcacgcttag cttgcgggcc gagcggcgga cgatctcggt
     5881 cagctcgggc aagctgtaaa accccaggtg ctcgcgcgtc gcaaaccggt cccgcagcgg
     5941 cgcggagagc atccccgctc gcgtcgtggc cccgatcagc gtgaagggct tgatccgcat
     6001 gttgatcgtg cgggcgttga tcccctcgcc cagcacgatg tcgaggcgaa agtcctccat
     6061 cgcggaatag aggtattcct cgaccgcctt cggcagccgg tggatttcgt cgatgaagaa
     6121 caccgcccgc tcctccatgt tggtgaggaa cggcagcaag tccttcggcg ccttgagggc
     6181 cgggccgctg gcggtcacca gcggcacatt cagctcgcgg ggaatgcagt tggcgaacgt
     6241 cgttttgccc agccccggcg ggccgtcgaa caaaatgtgc cccagcggct ccccccgctt
     6301 tcggctggca tccacgacga tgttcagtcg ctcgatcacg tcccgctgac cgaccatctc
     6361 cttaatccgc gccgggcgca ggccgttatc gaattcgtcg tcgcgcggcc cgtcggacga
     6421 gataatcggc tcgcgaggca tggaggctag acttggaagg tgtcgggtga gcgggacata
     6481 tggcgatgta ctcttgttac tgccgcgaat atatcagaca gcccgccgcg gcgtctgcgc
     6541 cgcaaaattc gccatggcgg gaactgtatt ctgtccgcgc gcgactctaa actattacct
     6601 gtccgcctcg tcactcgtcc cgccgagttc gcccgatggc cagtttcgct cccggtccgc
     6661 tggtcgatcc tgtccgggct ctgggcggtt gtccgagcga cgaacaattg ctggcgctca
     6721 ttccgggtga cctggctccg gagctcgctt cgtccgttac cgcgcacttg gaaacgtgcg
     6781 tctcgtgcca atcgcggctg acgaccttgc ggcatcgctc gcggaccgcg ccgcttgagc
     6841 ccaccacgcc tccgccgcgg ctcgatcgcc gcccctctga tccgccagcc gccggccccg
     6901 atgcgccgac catctacttg ccgcagcctc ccttgcgcgt gccagagcgg atcggccagt
     6961 acaagctgct caagctcatc ggccgcggcg gcatggggct ggtctatctg gcccagcacg
     7021 tgaagcttgg ccggacggtc gcgatcaagc tgctgccggg attgcaattg agcgacaaga
     7081 gcgcgatcgt ccgcctgcag cgcgagatcg ccgcggccgg ccgcgtgcag catccgaaca
     7141 tcgtctttgc caccgacgcc aacgaagagg acggcatcga ctacctcgtg atggaataca
     7201 tcgatggagt cgatatcggc cggctggtcg cggtgctcgg tcccctgccc tgccccgcgg
     7261 catgcgagat catccgccag gcggcgctcg gcctggccca cattcacgcc tgcggcctgg
     7321 tgcatcgcga tctgaaaccg tcgaacttga tgctcgcctc ggacggagtc gtgaagatcc
     7381 tcgatctggg gttggcgcgg ctgcgcgaag ggcagccggc cgacgacgac tcgcaaacgc
     7441 agaccggcta cctgctgggc acagccgact acgtctcgcc cgagcagctg cacgatccgc
     7501 acgacgccga cgtccgctcc gatctgtaca gcctgggctg caccttcttc aagctgcttg
     7561 ccggccaggc cccgtacagc ggcaccgatg ggggcagcat cggcaagaag ctcgacgccc
     7621 atcgctacgc cgccattccg tcgatccggt cgctgcggcc cgacgtgccg gaggaggtcg
     7681 atgcggttct gtcccggctg ctggccaagg agcccggcga ccgcattcag cagccgtccg
     7741 agctggccga gctgcttgcg cctctgtcgg ctgggtccga tttgtgcggt ttgtaccggc
     7801 ctgggcggga gcaggcggag atcgacgagc cgccccctcc cctgcccttg cagacgccgc
     7861 tgcccgatgg cggcagcacg gcggtcaaag gcagaacgcc cacgccgacc cgtttgctcg
     7921 ccgccgagcc gccgcggaag aaaacctgga cgtggatctc caccgcagcc gtcgccttgt
     7981 gcctgctcgc ggcagcgggc acgaccggct gggccattgc ccatctcacc ggtctgacgg
     8041 gaatccagcg gaaggaccaa ccgatccatg gccgaggccc gcagacgttc gatctcagtc
     8101 gtccgctgga agaaatcgcg tgggagggct acgaatcctc tccgcccgcg aggtacaacg
     8161 atggccagcg aatgctggaa ttgcggtccg agtcgtacca gctgatcaag ctcggtgatt
     8221 acgacggccg tccgggcacg ttcgaggtga cgatccgcca gacaccgtgg cacggctttg
     8281 ccgggctctt ttttggctat cgcgtgcagc cgcggagaga cgttcctcaa ttggcttcca
     8341 ccttccagct ggtatggctg caccacttcc cgctgaagga tgggaatggc aggaagtatg
     8401 gcgagcagat ggaagccatg cgccaacgaa ctgtgctcgg ggacgatacg cagccgttta
     8461 tcagggaggc ggcgatcggg caacgggttc ctctgccggc gcgggcgcaa gttcgcctga
     8521 aagtgcaatt tggccggcag ggctgcgaga aaatctcggt ggacgacgtg gttgtcgagg
     8581 atctcacggc ggccaacatc aatcagaaat acgacgcgat agactaccaa ggtccttggg
     8641 gtctgtttag cgcacgcggt tacggaccca aagaggctac ctggttcggc aacttgagtt
     8701 tcacaccctt ggagaattga accatgtctt gcatgatcga ctatcccacc gccggtggag
     8761 aaggcgccgt ggacgctggc ggtttcctcg tcagttgctg tggccagtgg ctgcgtccca
     8821 acacgtggga ccccgtagcc ccgtgtcgca ttgtggccaa ggtctatgca gccggcgcag
     8881 cggtagatgc aggcgtgccg aatgacggca ccgaaggtac gctgctgccg gacaacatgg
     8941 cttcgggcgg cgacttccat ttcaacatct ccctgattcc cgacgcaacg gtcggcggtg
     9001 cgcatcagat caaggtctgg ctgcaaaacc tcgataccgg gcaattcaac gccgacggtc
     9061 caatcgactt caacggatgt gagttcggtg tcggcggctg cgccgatcca ccttgcctcg
     9121 gacaggtcga aactgtgccg tgcgaactcg cgatcgatca gcctgcccca caagagtatc
     9181 gcgttggccc ggatttgcgc gcgacgtcgt tcatcggtga ggcgctggat gcctggaagc
     9241 aaggcgaact gaaattcgcg ggaatcaagc tggagtattc gtttccgctg tccagtccct
     9301 cgcgcgccgt ctggaccacg ggcggcgaca gccgggccct acggctggag gtcgtgcgcg
     9361 ccgcgagcgg cttcgtggcg acgctctacc tcctgcaagc gtccaatcgc ggcgtggaac
     9421 cgccgcggcg ctggcggtcg aaggatttca acctgaatcg cggcggcgtg ctgcgggccc
     9481 tcacggcctg ctgcaaggaa accgacgcca caatctacgt cagcgtgcct gacggaaaga
     9541 aacactgcga acctgagcgg accgtcgtct gatcaagcgc cgcagccctc actccgtcga
     9601 cgtctccagg cccgccttgg tgatcaggct ggagagcgtg gtcagggtca tggtgttgct
     9661 caacatgcgc accgagccgt c
//
LOCUS       DASRJB010000766         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_508941, whole genome shotgun sequence.
ACCESSION   DASRJB010000766 DASRJB010000000
VERSION     DASRJB010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508941"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFB80_20280"
     CDS             complement(<1..317)
                     /locus_tag="VFB80_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36184.1"
                     /translation="MHPTHTPILLHARDPRRGELVVACSDAALARGVRLNMPLAEAAA
                     LAQRGGACVIQPHDPAADLAALTRLAEHCERFSPLVGWQTVEGSKAATNGRGGQGSGV
                     GGR"
     gene            complement(385..1281)
                     /locus_tag="VFB80_20285"
     CDS             complement(385..1281)
                     /locus_tag="VFB80_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36185.1"
                     /translation="MNPPAPVREAQQALVATLRREVGRLEGVRPPEDDRPLSTGVEAL
                     DRLLPAGGLRRGTLVEYLSPGSGCGAPALALAAAREACRPGRALVVLDCSSRLRRKRP
                     ATESRATGFYPPAAAAWGIDLSRLLILRPANEADALWALDQALRCPGVGAVWAACDRL
                     DVRDFRRLQLAAETGGTLGLLLREGRLRGQPTWADVQWRVSPRCRERPLWRSGRADST
                     SSPRGSALADHRCAAVPASLTHPTWRLRVELLRCRGGAGGQSVVLELDETAATWREAT
                     DHAPHSLPVAAELADSAPVRRA"
     gene            1734..2888
                     /locus_tag="VFB80_20290"
     CDS             1734..2888
                     /locus_tag="VFB80_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN36186.1"
                     /translation="MMRSWPPNELLCCFLWQAVAVLAAATEPEVPGLPPALVLDHGGL
                     FRPQALGGRDLILLTLAFSPDGKTIASAGGGPGEKDAPAQGEVKLWEVATGRLLRTLT
                     VENGIVFHAEYSPDGQCLATASGPGTSTRTTPGEIRLWSPATGRLAGKLTGHDRGAYV
                     AAFSPDGRRLASGGIATIDASRPAAVRGGNATGDLKLWDLQTGRELWTGRGHSGAVAG
                     LVFSPDGKTLASSGGLSDGNVKLWDAAAGTQRRTFPCEAEVVRPVAFGPKSDSLTILS
                     FGLTGPQPPQPFTVQISRWNLIETQPLEAVSIRNGNAYRLALSHDGGLLACACHDGVK
                     VYDVRRQVELCSIPSQLRTRPVSFSPDDSLLAAGCDDGTVKVWNVAGIRK"
     gene            2924..3544
                     /locus_tag="VFB80_20295"
     CDS             2924..3544
                     /locus_tag="VFB80_20295"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZN36187.1"
                     /translation="MRIGILADIHEAIEPLRRALVEFRRMGVDQVVSLGDACDTCGTV
                     GRAGETVALLREARAVGVWGNHDFGLCGDVPSELRQRAAPGVLDFMASMQPHLVLEGC
                     RFSHVEPWLDACQIENLWYFDGLPDTAEKAGRSFAAVPERHLFVGHMHCWLVITPTGR
                     LDWNGEAPLLLNSEPRYLVVVAPVVDGWCAVYDTAAAELTPVRCAG"
BASE COUNT          631 a         1242 c         1268 g          602 t
ORIGIN      
        1 tcccgacccc cgacccccga cccctggcca ccgcgaccat tggtcgcggc tttggagccc
       61 tccaccgtct gccagccgac gagcgggctg aaccgctcgc agtgctcggc cagccgggtc
      121 agcgcggcca ggtcggcggc gggatcgtgc ggctgaatga cacacgctcc gccgcgctgg
      181 gccagcgccg ccgcttcggc cagcggcatg ttcagccgca cgccgcgcgc cagcgccgca
      241 tcgctgcaag cgacgaccag ctctccccgc cgtggatcgc gggcgtgaag gaggatgggc
      301 gtatgtgtag ggtgcatgag cggagcgaaa tgcaccttgt tggacggcga aaggtgcatt
      361 gccctgcgct gatgcacctt acgatcacgc gcggcgcaca ggcgctgaat cggccagttc
      421 ggcagccaca ggcagagaat gcggcgcatg atcggtagcc tcccgccagg tcgcggctgt
      481 ttcatcgagc tccagaacca cgctctgccc gcccgccccg ccgcggcagc ggagcaattc
      541 gacgcgaagc cgccaggtag ggtgggtcaa gctcgcgggc accgccgcgc agcggtggtc
      601 ggcgagcgca gacccacgcg gggacgacgt cgaatcggcg cgcccggaac gccacagagg
      661 gcgttcccta cagcgcgggc tcacccgcca ctgcacgtcg gcccaggtgg gttggccgcg
      721 gaggcggccc tcgcggagga gcaggccgag cgtgccgccg gtctcggcgg cgagttggag
      781 acggcggaaa tcgcgaacgt cgagccggtc acaggcggcc cacaccgcgc cgacgccggg
      841 gcagcgcagc gcctgatcga gggcccacag agcatccgcc tcattagccg gccgcaggat
      901 caagagccgc gacagatcga tgccccaggc cgcggccgcg ggggggtaga aacccgtagc
      961 ccgcgactcc gtcgcgggcc gcttgcgacg gagtcgcgag ctacagtcga gcacgaccag
     1021 cgcccggcct gggcggcagg cttcgcgggc ggcggccagg gccagggccg gcgcaccgca
     1081 gccgctgccg ggcgagagat attccacgag cgtcccccgc cgcaagccgc cggcgggcag
     1141 aagccgatcg agggcctcca ctccggtcga aagcggccgg tcgtcctcgg gcggccgaac
     1201 tccttccagc cggccgacct cgcggcggag cgtggcgact agcgcctgct gggcttctcg
     1261 gactggggcg ggcgggttca tggcgatgcc cagttatgca tgccgcaacc tggttcccag
     1321 gctccgcctg ggaacccact gctccgaggc tcctgcctcg cctggcagcg agggaggccc
     1381 tcgcggtagt gcgttaccag ggagaccctg gtaacgagtt atgcagggtt atgcatgcat
     1441 aaacgcacaa atttttgggg acgatgcatt tgacgtaagt ggcttgccgt caatggtttg
     1501 cgtcgcattc gtggcatccg cgattatgca agttatgcag tttaagcatc catatgcagg
     1561 gtgctagcaa tcgcctgctg ccagtgtaca atacacgcag tgtactgtag tatcaaacca
     1621 tcgtcaatat ccaaatcaat gtttatggac aattgatcta aattggcttg gcgcagccgc
     1681 gcattcaggg cccatttaca aattaaataa gcccatctcg ccgtcagcaa aacatgatgc
     1741 ggtcttggcc accaaacgag ctgctctgct gcttcctctg gcaagccgtc gccgtgctgg
     1801 ccgccgcgac cgagccagaa gtgccgggcc ttccgcccgc gctcgtcctg gatcacggtg
     1861 gcctgttccg gccgcaggcg ctgggcgggc gcgatctcat cctgctcacc ctcgccttct
     1921 cgcccgatgg aaagacgatt gcttccgctg gcggtggtcc cggcgaaaaa gacgctcccg
     1981 cgcaaggcga agtgaaactc tgggaagtcg ccaccggaag gctgctgcga acgctgaccg
     2041 tcgaaaacgg catcgtcttc catgcggaat attcgcccga cgggcaatgc ctggcgactg
     2101 cgagcggtcc gggcacttcg acccgcacga caccgggcga aattcgcctc tggagtcccg
     2161 ccacgggccg gctcgctggc aagctcacag ggcacgaccg cggcgcttac gtagcggcct
     2221 tcagccccga cggcaggcgg ctggcctccg gcggtatcgc cacgatcgat gccagccggc
     2281 cggccgctgt tcggggcggc aacgccacgg gtgacctgaa gctttgggac ctgcagacgg
     2341 gccgggagtt gtggacggga cgtgggcaca gcggtgcggt cgccgggctg gtcttctcgc
     2401 ccgacggcaa aaccctcgcc tccagcggcg gcttgtccga cggcaacgtg aaactatggg
     2461 acgcggctgc cgggacgcag cggcgaacct ttccctgcga agccgaagtc gtccgccccg
     2521 tggctttcgg cccaaaatcc gattccttga ccattctcag ctttggcctt accggcccgc
     2581 agccacctca gccattcacc gtgcaaatca gccggtggaa tttgatcgaa acccagccgc
     2641 tcgaagccgt cagcattcgg aatggcaacg cctatcgcct ggcgctttcg cacgacggcg
     2701 gcctgcttgc ctgtgcgtgt cacgacggcg tcaaagtgta cgacgtaagg cgacaggtcg
     2761 agctgtgcag catcccctcg cagctccgca cgcgcccggt gtcgttctcg cccgacgaca
     2821 gcctgctggc cgccgggtgc gacgacggca cggtgaaggt ttggaacgtt gccgggatcc
     2881 gcaagtagga ttgtcgtggc ggttcgacga aacggagcct gccgtgcgga tcggaattct
     2941 cgcggacata cacgaagcga tcgagccgct gcggcgggcg ctcgtcgagt tccgtcggat
     3001 gggcgtggac caggtcgttt cgttgggcga cgcctgcgac acttgcggca ccgtgggccg
     3061 ggcgggcgaa acggtggcgc tcctgcgcga ggcccgcgcg gtcggcgtgt ggggcaacca
     3121 cgatttcggc ctctgcggcg acgtacccag cgagctgcgg cagagggccg ccccgggtgt
     3181 gctggacttc atggcctcga tgcagccgca cctggtcctg gagggctgcc ggttcagcca
     3241 cgtcgagccc tggcttgatg cgtgccagat cgaaaacctg tggtacttcg acggccttcc
     3301 cgacacagcg gaaaaagcgg gccgcagctt cgccgccgtg cccgagcggc atctcttcgt
     3361 cggacacatg cattgctggc tggtgataac gcccaccggc cggctggatt ggaacgggga
     3421 agcgccgctt ttgttgaaca gcgagccgag atacctggtg gtcgttgcgc cggtcgtgga
     3481 cgggtggtgc gcggtctatg atacggccgc tgccgaattg acgccggtgc gctgcgccgg
     3541 ctgattcgcc gcggcgctcc gctgccgagc tgatagcgag cgcagcgttg tcaacgcacg
     3601 agcaactgat attgcggtgc gtcgtcgcct gattcttcga ccaggcgaaa cgacttgcca
     3661 aagacagccg atttgaccca gccggcactc ttgcgcgtag cgacatagcg gcggcccgag
     3721 cggcggagga tgtcgaacga gag
//
LOCUS       DASRJB010000767         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1910887, whole genome shotgun sequence.
ACCESSION   DASRJB010000767 DASRJB010000000
VERSION     DASRJB010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910887"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VFB80_20300"
     CDS             <1..980
                     /locus_tag="VFB80_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZN36188.1"
                     /translation="APPLAIRDMSSSSSLGFGIGLRTAHFNTLLERRAEVDWFEALSE
                     NFMMEGGRPHFVLDRIRRDFPIVLHGVSLSIGSTDPLRRDYLDRLRWLIERFEPRWIS
                     DHLCWTGVLGRNTHDLLPMPLDAASLRHVVSRIRTVQDFLERPLVLENPSSYVTFTAD
                     TMPEWEFLSRLAEGTGCRLLLDVNNVYVSSRNHGFDPQEYLRSIPAERVQQFHLAGHT
                     DLGTHCIDTHDGRVVDAVWDLYRQAAQLTGGAATLLEWDAKIPTFDEVWAEAEKARQY
                     LARAQFERTEEPKNQMSENSNFGSSVLGSSEFASRAFPHPAHLVAAEAE"
     gene            977..1768
                     /locus_tag="VFB80_20305"
     CDS             977..1768
                     /locus_tag="VFB80_20305"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HZN36189.1"
                     /translation="MNAPAQLAAVQQWLLATIADPAPIDAAAVCNRLTPGPQQSPEQR
                     LSIYQHAYFARLLDVLRELFPCTRFAVGDETFDELATGYLLRHPPRSYTLARLADRFG
                     EYLDETRPRDEPWSRFVVELVRLEEAIDRIFDGPGPERLPPFALPAGADARLQLRLVP
                     GFELHTFSFPVSDFYTDWKAGRKPAWPQPQEQHVALLRRDYVVRRYDLTAVQAALLTA
                     IRGGAALQDALAAAAACEPAAAFEELSALVRRWFTHWSAAGFFAA"
     gene            1860..1941
                     /locus_tag="VFB80_20310"
     tRNA            1860..1941
                     /locus_tag="VFB80_20310"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1891..1893,aa:Ser,seq:cga)
     gene            complement(1981..>3279)
                     /locus_tag="VFB80_20315"
     CDS             complement(1981..>3279)
                     /locus_tag="VFB80_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36190.1"
                     /translation="KLAGSAAASLVALPAGGEQPASKPRVAAVFTEFRRLSHAYHILG
                     NAMGKYLFRGQWVDPGLDVVSFYADQFPAGDMARDVSQKFKVPLFKSIDEALCVGGRE
                     LAVDAVLLIGEHGRYPANELGQRMYPRKEFFDQIVAVQRRAGRFVPVFNDKHLSYRWD
                     WAKAMYDEAQRLKMPLMAGSSVPLAQRMPPLELPPDAAVIEAVSIHGGGVEGYDFHAL
                     EVMQSLIEARAGGETGVSRVEFLTGEALQRAAAEGRWPTALVAAAMRAERDAKMPRRS
                     ITGRPPRPFPDEPFAPRHAILVTYNDGTRGTVLTIGRSDERWNFACQLRGDTKPLATS
                     FYGGPWGNWNLFGALGGAIAHFFRTGKAPYPVERTLLVSGILDAAMHSRHAGGQPQET
                     PHLKLKYAAQDFRAFCETGESWQVLTTDSIPPQDFQPGSG"
BASE COUNT          512 a         1135 c         1064 g          568 t
ORIGIN      
        1 tcgcgccgcc gctcgcgatc cgcgacatgt cttcatcgtc gtctctcggc ttcggcatcg
       61 gccttcgcac cgcacacttc aacacccttc tcgaacgccg cgccgaggtg gattggttcg
      121 aggcgctctc cgagaatttc atgatggagg gcggacgacc tcacttcgtc ctcgaccgga
      181 tccggcgcga ttttccgatc gtcctccacg gcgtctcgct ttcgatcggg agcaccgatc
      241 ccttgcggcg ggactacctc gaccgcttgc gctggctgat cgaacgcttc gagccgcgct
      301 ggatctcgga tcacctctgc tggacgggcg tgctcggccg caacacccac gacctgctgc
      361 cgatgccgct cgacgccgcc tcgctgcgcc acgtcgtctc acgcatccgc accgtgcagg
      421 acttcctcga gcggccgctg gtgctcgaga accccagcag ttacgtcacc ttcaccgccg
      481 acacgatgcc ggagtgggaa ttcctttcgc gattggccga ggggaccggc tgccgcctgc
      541 tgctagacgt gaacaacgtc tacgtcagca gccgcaacca cggcttcgat ccgcaggagt
      601 acctgcggtc gattccagcc gagcgggtgc agcagtttca cctggcgggg cacaccgacc
      661 tgggcacgca ctgcatcgac acgcacgacg gtcgcgtcgt cgatgccgtg tgggacttgt
      721 atcgccaggc cgcgcagctg accggcggcg cggcgacctt gttggaatgg gacgcgaaga
      781 ttcccacgtt tgacgaagtt tgggccgaag cagaaaaggc gcgtcagtat ttggcccgag
      841 cacaattcga aagaaccgaa gaaccaaaga accaaatgag tgagaactcc aacttcggtt
      901 cttcggttct tggttcttcg gaatttgctt cccgcgcctt tccccatccc gcccacctgg
      961 tcgctgcgga ggctgaatga acgcgccggc acaactcgcc gcggtgcagc agtggctcct
     1021 ggccacgatc gctgatcccg cgccgatcga cgcggcggcg gtctgcaatc gcctcacccc
     1081 gggcccgcag cagtcgcccg agcagcggct gagcatctat caacatgctt acttcgcgcg
     1141 attgctcgat gtgctgcgcg agctgttccc ttgcacgcgg tttgccgtcg gggacgagac
     1201 gttcgacgaa ctggctaccg gctatttgct gcggcatccg ccgcgaagct acacgctggc
     1261 tcgcctggcc gaccgctttg gcgaatacct cgacgagacg cggccgcgcg acgaaccctg
     1321 gagccggttc gtcgtcgagc tcgtgcgact ggaagaagca atcgaccgca tcttcgacgg
     1381 gccgggcccc gaaaggctgc cgcctttcgc gctgcccgcc ggcgcggacg cccgcttgca
     1441 gctgcggctc gtgcccggct tcgagctgca cacgttttcg tttcccgtga gcgacttcta
     1501 caccgattgg aaagcgggcc gcaagccggc ctggccgcag ccgcaggaac agcacgtcgc
     1561 cctgctgcgc cgcgactatg tcgtgcggcg ctacgactta accgcagttc aggccgcgct
     1621 gctgacggca atccgcggcg gcgcggcgct gcaggacgcc ctggccgcag ccgccgcgtg
     1681 tgagcccgcc gcagcattcg aagaactgtc cgcgctcgtc cgccgctggt tcacccactg
     1741 gtccgccgcc ggctttttcg ccgcctgacg gccgaaggtt tttgccgccc gacgctgcag
     1801 tgggccgccg tgcgcgaacc tctcttttcc ccgagggttc ctatggtaaa atcgccctcg
     1861 gagggtaagc gaatggtcag cggtctgatt cgaaatcaga tgccccgcaa ggggttgcgg
     1921 gttcgactcc cgtgccctcc gctcggatga ggccggccaa ctgcaacgga gagtggatcg
     1981 tcacccgctg ccgggctgaa agtcctgggg cgggatggag tcggtggtga ggacctgcca
     2041 gctctcgccg gtctcgcaga aggcgcggaa gtcctgggcg gcgtacttca gcttcaggtg
     2101 cggcgtctct tgcggctggc cgccggcgtg gcgggagtgc atggccgcgt cgaggatgcc
     2161 gctgaccagc agcgtgcgct cgaccggata gggcgccttg ccagtgcgaa agaagtgggc
     2221 gatcgcgccg cccagcgcgc cgaacaggtt ccagttgccc cagggcccgc catagaacga
     2281 cgtggccagc ggcttcgtat cgccgcgcaa ctggcaggca aagttccacc gctcgtcgct
     2341 gcggccgatc gtcagcaccg tgccgcgtgt cccgtcgttg tacgtcacca ggatcgcgtg
     2401 ccgcggcgcg aagggctcgt ccggaaaggg ccgcggcggc ctgccagtaa tactgcggcg
     2461 cggcatcttg gcgtcgcgct cggcgcgcat ggccgctgcc accagcgccg ttggccagcg
     2521 gccttccgcc gccgcgcgct gcagggcctc gcccgttaga aactcgacgc ggctgacgcc
     2581 ggtctcgcct cccgcgcggg cttcgatcag cgactgcatc acttccaggg cgtgaaagtc
     2641 gtagccttcg accccgccgc cgtgaatcga gacggcctcg atcacggccg cgtccggcgg
     2701 cagctccagc ggcggcatcc gctgggccag cggcacgctg ctgccggcca tcagcggcat
     2761 cttcagccgc tgggcttcgt cgtacatggc tttggcccag tcccagcggt acgagaggtg
     2821 cttgtcgttg aagacaggca cgaagcggcc ggcgcggcgc tgtacggcga cgatctgatc
     2881 gaagaattcc tttcgcgggt acatccgctg gcccagctcg ttggcggggt agcggccgtg
     2941 ttcgccgatc agcaggaccg catcgaccgc gagctcccgg ccgccgacgc agagcgcctc
     3001 gtcgatcgac ttgaagagcg ggactttgaa cttctgcgac acatcgcggg ccatgtcgcc
     3061 cgccggaaac tgatcggcgt agaacgagac cacgtccagt cccggatcga cccactggcc
     3121 ccggaacagg tacttgccca tcgcgttgcc gaggatgtga taggcgtgcg acaggcggcg
     3181 gaattcggtg aacaccgccg cgacgcgcgg cttggacgcc ggctgctcgc cgccagcggg
     3241 cagcgccacc agggaggctg ccgccgatcc ggccagctt
//
LOCUS       DASRJB010000768         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3326335, whole genome shotgun sequence.
ACCESSION   DASRJB010000768 DASRJB010000000
VERSION     DASRJB010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326335"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFB80_20320"
     CDS             complement(<1..554)
                     /locus_tag="VFB80_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36191.1"
                     /translation="MHPIQEHSLRETRRYFFGRMAAGIGTAALSSLLAPQLAAVESSS
                     EQDSLGLLPKLHHAPKAKRVIWLFMADGPSQLDLWDYKPKLAEFFDKDLPDSIRQGQR
                     ITTMTSGQARLPVAPSMFKFAQHGQHGAWVSELLPKLAGVVDDLCVIKTMNTEAINHD
                     PAITYIQTGSQIPGRPSLGAWLSYG"
     gene            complement(557..3724)
                     /locus_tag="VFB80_20325"
     CDS             complement(557..3724)
                     /locus_tag="VFB80_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN36192.1"
                     /translation="MRAAARCVLVCCLGVFFARASAAEEKVDFNRDIRPILSNTCFKC
                     HGFDDKTRQADLRLDVPEGATKKLESGAVAVVPGKLAESELVRRITAADPAERMPPAD
                     SGKSLKPEQIELLKRWVAEGAELKQHWSFLPPVRPTPPVVTPAAERIANPIDQFISAR
                     LEKAGLSPSPPADKVTLLRRVTLDLTGLPPTPAEIDRVLSSIAPSPPLPLSPSEAEGE
                     SGREGEWEKVYEAYVDKLLASPRYGEHMARYWLDAARYGDTHGLHLDNERSMWPYRDW
                     VVRAFNENLPFDRFTVEQLAGDQLPEATLDQLVASGFNRCNVSTSEGGSIDEEVRVRY
                     AVDRTETMSTVFLGLTLGCAVCHTHKFDPVSQKEFYQLYAFFNSAADAAMDGNALSPP
                     PIKKLATPEQAAQLKALDEQIAGVNKQTAELLASIEYTDPGASESAAAAEPKEFVWID
                     DAAPSGAKLEGNSEWKFVTKENGQVFSGEKATTRKASDLSQHFFTGASPGLKVGEGDK
                     LFAYVYLDPADPPQEIMLQWNDGSWEHRCYWGSDVIPWGGANSASRLPMGKLPQAGEW
                     VRLEVEAAKVGLAAGAVINGWAFTQHGGTTFWDKAGIVTRTPQVGAGFESLAAWEAFE
                     KSQKKSDVPGDVKEALKVETEKRNDDQKKKIRDYFVQNVCTTTRPKFDPLRKQLEELN
                     KQKGRVDGTIPVSLVMADMPAKRDTFILVRGAYDKPGEKVEANVPAILPPLPEGAPRN
                     RLTLARWLVDPQHPLTARVTVNRFWQQYFGTGIVKTAEDFGSQGSWPTHPELLDWLAR
                     DFIDCGWDVKRLQKLIVMSATYRQSSRVTKELLARDPANELLARGPRFRLDAEVIRDS
                     ALFTSGLLIEQPGGKAVKPYQPEGLWEAVGFQGSNTAKYKPDGGAANYRRTLYTFWKR
                     TAPPPSLMTFDAPSREACTVRRSRTNTPLQALALMNDVQYVEAARKLAERVMTEGGAA
                     PAERLAHGFRLATSRQPTADEAAILVAGYEAHLKKYQADKAAAEKLIALGESKRNAAL
                     DAAELAAYTLVCNLILNLDEVVTKE"
     gene            3914..4774
                     /locus_tag="VFB80_20330"
     CDS             3914..4774
                     /locus_tag="VFB80_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36193.1"
                     /translation="MHWFWYLVLGYWVLGAAGSIASFRLYVNDEEDPNFWRAMQADPV
                     YVWVALLFAAVIVGPLFPLILVHCAWKGWRESRSASREWQKFRRTFRPIEFEALHPAN
                     LPADVQQHFEKCSPILAGLGFVPVVTCRLKPEPRPLLALLLLGREGAVQAEASWIFGT
                     PAAAFVSVLEDGHVLETACVETSLPQTEIDAINRSGTFSTQMFAAGEGGNIADEAFLA
                     AAYRSHLQLLAELENRLGCGTLHLAADQIPDLKRYENAVFGELKFVRGKVDNRPQPQP
                     CPKGIARRIG"
     gene            complement(4821..5252)
                     /locus_tag="VFB80_20335"
     CDS             complement(4821..5252)
                     /locus_tag="VFB80_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36194.1"
                     /translation="MFKTILRSLFGPPREPFHDPVLGELKPASVGWSVRVTRGRDSFE
                     FTIGGAKQPDAALLAHAREILSNFESFQISVAKCVAAESRDYPDDVKAELAGLQIDNI
                     SLCWPDRPDDGMIFFRSPKNDAACWRCDYIARKPQGLGCDT"
     gene            5383..7683
                     /locus_tag="VFB80_20340"
     CDS             5383..7683
                     /locus_tag="VFB80_20340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN36195.1"
                     /translation="MPRDRLWTFVVVCLLVTAAAGPMEAAGAAETPAILIGVAQGPSR
                     APAALRQGAVLPPQSVSAVDVGDDGRFVAVGTMACRHDRNFWLLSAETGDVAWGRYVE
                     TWAPAQVRALAKGQGFAVGLTYGPVTSVGSTVGLFHDERDPISYVYDWPLVGGRGWLR
                     YGGGDWQTGWPASIPADLFTRAGEAVFASADANNSRSVFRFDGGPPQSLDVSRPFRMA
                     ASADGKVVALAYAVHDFRGVEPKVAGRFDGAAPHALVSVRSAAGAAPWKELWRGEGAA
                     GTAPAPEPPEPSAEFPALAESFNLKPLALVPFRIPLSVAVNGDGSQVALAEYGGHSRV
                     GRERILPRWSPRDPIAFCPRQRGLLRVLAGGQELVAAALPTLGLFDVHLDQPGETVWC
                     VPASWFARGLAGCPWLSADEDADTVFIYDLARKAWSAPWRFPDAVSDFAVHPDGTRAL
                     VSCWDGWLYLVGRDGGLIAKVQVGGSARLRWSPDRKFAVCGTQEGVVVRINADGAEVW
                     RKQLSAAQAARPGPEFGPVFKELPVYSVGRRGPEHAYVGDMWLIKTALGGILVDTGGV
                     SAQAATRERIRAAGVDPQDIRYVLLSHSHGDHAGAAYLWRAAGAKVVAPASAAFAVTG
                     VMPTWSDYNLWVPCPIDEPLPLKRAGDEAEITLCGVKVKAIFAPGHSPDSVIYSMELG
                     GRRVIFTGDIAFDDRRPGLPLGSNILHRCWGHHENAARVIEIFESQVLPLAPEFNFTG
                     HAAYPDATAAWKRILDASRTALKPGE"
BASE COUNT         1248 a         2653 c         2652 g         1434 t
ORIGIN      
        1 ccgtagctca gccacgcgcc gaggctcggc cggccgggaa tctggctgcc ggtctggatg
       61 taggtgatcg ccgggtcgtg gttgatcgcc tcggtgttca tcgtcttgat gacgcacagg
      121 tcatcgacga cgccggccag cttcggcaac agctcgctga cccacgcccc atgctggccg
      181 tgctgggcga acttgaacat cgacggcgca accggcagcc gggcctggcc gctggtcatc
      241 gtcgtgatcc gctgcccctg gcggatgctg tcgggcaagt ccttatcgaa aaactccgcc
      301 agcttcggct tgtaatccca cagatcgagc tggctcggcc catccgccat gaacagccag
      361 atcacgcgct tggccttcgg cgcgtgatgc agctttggca acagcccgag gctgtcctgc
      421 tcggagctcg attccaccgc ggccagttgc ggcgcgagca gcgacgagag cgccgccgtg
      481 ccaatgccgg cggccatgcg cccgaaaaag tagcggcggg tctcgcgaag cgagtgttct
      541 tgaatgggat gcatgcttat tccttcgtca ccacttcgtc gaggttcagg atcaagttgc
      601 agaccagcgt gtacgcggcc agctccgcgg cgtccagcgc ggcgttgcgt tttgattcac
      661 cgagggcgat cagcttttcc gcggccgctt tgtcggcctg atatttcttc agatgggcct
      721 cataaccggc gaccagaatc gccgcttcgt cggccgtcgg ctgccgcgag gtcgccagcc
      781 ggaagccgtg cgccagccgc tcggcgggcg ctgcgccgcc ctcggtcatg acccgctcgg
      841 ccagcttgcg ggccgcctcg acgtactgca catcattcat cagcgccagg gcttgcagcg
      901 gcgtatttgt gcggctgcgg cgaacggtgc aggcctcgcg cgacggcgcg tcaaaggtca
      961 tcagcgaggg cggcggcgcg gtccgtttcc agaacgtgta gagcgtccgc cggtagttgg
     1021 ccgccccacc gtctggcttg tacttcgccg tgttgctgcc ctggaagccg accgcctccc
     1081 agaggccctc gggctgatac ggcttcaccg ccttgccacc gggctgctcg atcaagaggc
     1141 cgctggtgaa cagcgccgaa tcgcggatca cctcggcgtc cagccggaac cggggccctc
     1201 gggcgagcaa ctcgttcgcc gggtcgcgag ccagcagctc cttcgtgacc cgcgacgact
     1261 gccggtacgt ggcggacatc acgatcagct tttgcagccg cttgacgtcc cagccacagt
     1321 cgatgaagtc acgggccagc cagtcgagca gctccgggtg ggtcggccac gagccctgcg
     1381 agccgaaatc ctcggccgtc ttgacgatgc ccgtcccaaa atactgctgc cagaagcgat
     1441 tgacggtgac ccgggccgtg agcgggtgct ggggatcgac gagccagcgg gccagcgtca
     1501 agcggttgcg gggcgcgccc tccggcagcg gcggcaggat ggccggcaca ttggcctcga
     1561 ccttctcgcc cggcttgtcg tacgccccgc ggaccagaat gaacgtgtcg cgcttggcgg
     1621 gcatgtcggc catcaccagc gagaccggaa tcgtgccgtc cacgcggccc ttctgcttgt
     1681 tcagttcttc gagctgctta cgcagcgggt cgaacttcgg ccgcgtcgtc gtacagacgt
     1741 tctgcacgaa gtagtcgcgg attttcttct tctggtcgtc gttgcgcttt tccgtctcga
     1801 ccttcagcgc ttccttcacg tcgccgggca cgtccgactt cttctgcgat ttctcaaagg
     1861 cctcccaggc ggccagcgat tcgaagcccg ctcctacctg cggcgtgcgg gtcacgatgc
     1921 cggccttgtc ccaaaacgtt gtgccgccgt gctgcgtgaa ggcccagccg ttgatgaccg
     1981 ctccggctgc cagaccgacc ttcgccgcct cgacctccag ccgcacccac tcgccggcct
     2041 gcggcagctt gcccatgggc aggcgactcg ctgaattcgc gccgccccag ggaatcacgt
     2101 cgctccccca gtagcagcga tgttcccacg agccgtcgtt ccactgcagc atgatctcct
     2161 gcggtggatc ggccggatcg agatagacgt aagcgaacag cttgtcaccc tcgccgacct
     2221 tcaggcccgg cgaggccccg gtaaagaagt gctgcgagag gtcactggcc ttgcgggtcg
     2281 tggccttttc gccgctaaag acctggccgt tttccttggt gacgaatttc cactcgctgt
     2341 tgccctccag tttcgcgccg ctgggcgcgg cgtcgtcgat ccacacgaat tccttcggct
     2401 ccgcggctgc ggcggactcg ctcgcgccgg gatcggtgta ctcgatcgac gccagcagct
     2461 cggcggtctg cttgttgacg cccgcgatct gctcgtcgag cgccttgagc tgtgccgcct
     2521 gctcgggcgt ggccagcttc ttgatcggag gcggcgagag ggcgttgccg tccatcgcgg
     2581 cgtcggctgc cgaattgaag aaggcgtaga gctggtagaa ctccttctgc gacaccgggt
     2641 cgaacttgtg cgtgtggcag accgcgcagc ccagcgtgag ccccagaaac accgtgctca
     2701 tcgtctccgt gcgatcgacg gcgtaccgca cgcggacttc ctcgtcgatc gagccgccct
     2761 cgctggtcga gacgttgcag cggttgaagc cgctggcgac gagctggtcc agcgttgcct
     2821 caggcaactg atcgcccgca agctgctcga ccgtgaaccg gtcgaacggc aggttctcgt
     2881 tgaacgcccg cacgacccaa tcgcgatagg gccacatcga ccgctcgttg tccaggtgca
     2941 gcccgtgcgt gtcgccgtac cgcgccgcat cgagccagta gcgggccatg tgctcgccgt
     3001 agcgggggct ggcgaggagc ttatcaacat acgcctcata aaccttctcc cactctccct
     3061 ctctcccgct ctccccctct gcttccgagg gggagagcgg gagagggggt gagggggcga
     3121 tggacgaaag cacgcggtcg atctccgccg gcgtcggcgg caagccggtc aagtcgagcg
     3181 tgacccgccg cagcagcgtc accttgtcgg ccggcggaga tgggctcagg cccgctttct
     3241 ccagccgggc ggagatgaac tgatcgatcg ggttcgcgat ccgctcggcg gcaggcgtta
     3301 caaccggcgg cgtcggccgc accggcggaa ggaacgacca gtgctgcttg agctccgcgc
     3361 cttcggcgac ccaccgcttg agcagctcga tttgctccgg cttcagagac ttgccgctgt
     3421 cggccggcgg catccgctcg gccggatcag ccgccgtgat ccgccgcacg agctcactct
     3481 cggcgagttt gcccggcacc acggccaccg cgcccgattc gagcttcttc gtggctcctt
     3541 ccggcacatc cagccgcagg tcggcctgcc gggtcttgtc gtcgaagccg tggcacttga
     3601 agcaggtgtt gctgaggatc gggcggatgt cgcggttgaa atcgactttt tcctccgctg
     3661 cgctggcccg cgcgaagaac actcccaggc agcaaacaag cacgcagcgg gcagcagcac
     3721 gcatcggcag attcccgatt atccagaagg cgggaccgaa ccggcgggaa ccgtcgcggc
     3781 gacaacgccc acgccggctg aagcggcccc ccacagtgcc gctccgcccc cattacaacc
     3841 gaacaccccc cggctgtcca gcaaacgaac ggccgcgacc tagacttgtg atgtcgcggc
     3901 ggagggctgg cgcatgcact ggttctggta tctcgttctc ggttattggg tgctgggcgc
     3961 ggccgggtcg attgcgtcct ttaggctcta cgtcaacgac gaggaggacc caaacttctg
     4021 gcgtgccatg caggccgacc cggtgtacgt ctgggtcgcc ctgctgttcg cggcggtcat
     4081 cgtcgggccg ctgttccctt tgatcctcgt ccattgtgct tggaaaggct ggagggaaag
     4141 ccggagcgcg agccgcgagt ggcagaagtt ccgccgcact ttccgcccca tcgagttcga
     4201 agcgctgcat ccggccaacc tgcccgcgga cgtgcaacag catttcgaaa agtgctcgcc
     4261 gatcctggca gggctcgggt ttgtgccggt cgtcacctgc cggctcaagc cggagccccg
     4321 gccgctgctg gcactcctcc tgctggggcg ggagggcgcc gtccaggcgg aggcgagttg
     4381 gatttttgga acgcccgccg ccgcgttcgt ctcggtcctc gaagacgggc acgtgctgga
     4441 gacggcctgc gtggaaacga gcctgccgca aaccgagatc gacgcgatca accgctccgg
     4501 caccttcagc acgcagatgt tcgccgcggg cgaagggggc aacatcgcgg acgaagcctt
     4561 tctggcggcc gcctatcgca gccatttgca gctgctggcc gagttggaaa atcgcctcgg
     4621 ctgcggaacg ctgcacctgg ccgcggatca gattcccgac ctcaaacgct acgaaaacgc
     4681 cgtcttcggc gagctaaagt tcgttcgcgg gaaagtcgac aaccgccccc agccgcagcc
     4741 ctgcccaaaa ggaatcgccc ggcgtatcgg ctaactgtaa caggcggctc tctagaatcg
     4801 cagtaagaaa aaggcggggc tcacgtgtca catcccaggc cctgtggctt gcgggcaatg
     4861 tagtcgcatc gccagcaggc ggcgtcgttc ttggggctgc ggaagaagat cattccatcg
     4921 tcgggccggt ccggccagca gagggagata ttgtcgatct gaaggcccgc cagctccgcc
     4981 ttcacatcgt ccgggtagtc gcgcgattcc gcagcgacgc actttgccac ggagatctgg
     5041 aacgactcga agttgctgag gatctcgcgg gcatgggcca gcaaggcagc atcgggctgc
     5101 ttcgcgccgc cgatcgtgaa ctcgaaggaa tctcggcctc tggtgactcg gacggaccat
     5161 cccacgctcg ccggcttcag ctcccccagg accgggtcgt ggaaaggctc ccgcggtgga
     5221 ccgaacaagg acctgaggat tgttttgaac atgtcgcttg cgccgaccag cacgccgacc
     5281 gaccgcccca aacgaaccag agtaagccgc gtgcgtcggg aggggcataa aggtgtgagg
     5341 aacgtagaat atcccttttc gttcccgcga gagacatcca agatgccccg tgatcggctc
     5401 tggacttttg tcgtcgtctg cctgttggtg acagcagccg ccggcccaat ggaggcggcc
     5461 ggggccgcgg agacgccagc gatcctgatc ggggtagctc aagggccaag tcgcgcaccg
     5521 gcagcgctgc gccagggcgc tgtcttgccg ccgcagagcg tttcggccgt ggacgtcggc
     5581 gacgatggcc gcttcgtcgc cgtgggcacc atggcctgcc gccacgaccg caacttctgg
     5641 ctgctgtcgg ccgagaccgg cgacgtcgcc tggggccggt acgttgagac ctgggccccc
     5701 gcgcaggtcc gggcgctggc caagggccaa ggctttgccg tcgggctgac gtatgggccc
     5761 gtgacgtcgg tcgggtctac ggtcggcctg tttcacgacg agcgggatcc tatttcctat
     5821 gtctatgatt ggccgctcgt cggcggccgg gggtggctgc gctatggcgg cggcgactgg
     5881 cagaccgggt ggccggccag cattcccgcc gacctgttca cccgggccgg cgaggccgtc
     5941 tttgcttcgg ccgatgccaa taacagccgc tcggttttcc gcttcgacgg aggcccgcct
     6001 caatcgctcg acgtgagccg tccctttcga atggccgcca gcgcggacgg caaggtcgta
     6061 gcgctggcct acgcggtgca cgacttccgc ggagttgagc cgaaggtcgc cgggcggttc
     6121 gatggcgccg caccacacgc gctggtctcg gtccggagtg ccgcgggcgc tgcgccctgg
     6181 aaggaattgt ggcgcggcga aggggcggct ggaactgcgc cggcgcccga gcctcccgaa
     6241 ccatcggccg agttccccgc gctggcggag agtttcaatt tgaagccgct ggcgctggtg
     6301 ccgttccgca ttcccctgtc cgttgccgtc aacggcgacg gttcgcaggt ggcactcgcc
     6361 gaatacggcg gccactcgcg ggtcgggagg gagcgaatcc tgccgcgctg gagtccgcgc
     6421 gatccgattg cgttctgccc tcgccaacgc ggcctgctgc gcgtgctggc cggcggccag
     6481 gagctggttg ccgcggcgct gcccaccctt ggcttgttcg acgtgcatct ggatcagccc
     6541 ggcgagacgg tctggtgcgt accggcgagc tggttcgccc gcgggctggc tggctgccct
     6601 tggctttcgg ctgatgagga cgccgacacc gtcttcatct acgacctggc ccggaaggcc
     6661 tggagtgctc cctggcgatt cccggacgcg gtgagcgatt tcgcggtcca cccggatggc
     6721 acgcgggcgc tcgtttcgtg ctgggacgga tggctgtacc tggttggccg agacggcgga
     6781 ctgatcgcca aggtccaggt cggcggatcc gctcggctgc ggtggagccc cgataggaag
     6841 ttcgcggtct gcggcacgca ggaaggcgtc gtcgtccgga tcaatgccga tggcgcggag
     6901 gtctggcgga agcagctctc agccgcacag gccgcgcggc cggggcccga gttcgggccg
     6961 gtattcaaag agcttcccgt gtattcggtt gggcggcgcg ggccggagca cgcctacgtt
     7021 ggcgacatgt ggctcatcaa gaccgccctt ggaggaatcc ttgtggacac cggcggcgtg
     7081 tctgctcagg ccgccacgcg ggagagaatc cgggcggccg gcgtggaccc gcaggacatc
     7141 cgctacgtgc tcctgagcca cagccacggc gaccacgccg gggcggcata tctgtggcgg
     7201 gccgccggag ccaaggtggt ggctccggcc agcgcggcgt ttgcggtgac cggggtgatg
     7261 cccacctgga gcgactacaa cttgtgggtg ccgtgcccga tcgacgagcc actgccgctg
     7321 aagcgggctg gcgacgaggc cgagatcacg ctgtgcgggg tgaaggtcaa ggcgatcttc
     7381 gcgccgggcc acagccccga ctcggtcatc tactccatgg agctcggcgg ccggcgggtg
     7441 atcttcacgg gcgacatcgc cttcgacgac cgtcgccccg gactgccgct gggatcgaac
     7501 atcctgcacc gctgctgggg gcaccacgag aatgccgccc gcgtcatcga aatcttcgag
     7561 tcccaggtac tgccgctggc gccggagttc aacttcaccg gccacgccgc ctatcccgac
     7621 gccaccgccg cctggaagcg cattctggac gccagccgca ccgcccttaa acccggtgag
     7681 tagcgcgacg ccaacgctcc gcaacaaagc acctcagctt gcggtaaacc gcacggtact
     7741 ttctatgtgg acgggtgttt aataaacgtg gtactgcgcg cggccttttg tgttctttct
     7801 caatttagtg gatggatgtt tcgtaaaggc ggtgattttg ccgctgcgcg cccggcgcag
     7861 tcctgagtag gagtggcagc gggaggtaag gataattctc gggggaggtc tctaacgctt
     7921 caaacccaac tggctttcca gcggccaggt agcgatgcaa acggagagcg gacaaacact
     7981 tgcggcg
//
LOCUS       DASRJB010000769         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1911114, whole genome shotgun sequence.
ACCESSION   DASRJB010000769 DASRJB010000000
VERSION     DASRJB010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911114"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="VFB80_20345"
     CDS             <1..2564
                     /locus_tag="VFB80_20345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36196.1"
                     /translation="LEGKRVGAESRPAQMAARGFQYGVTPRRSRGNWLTAYQATGGKA
                     LWTRSASDEDKEGSTDIGFLAAPTPCGSLLLAPITDGGTIWLLGLERGTGKTLWRSYL
                     CDEPQGGASPWSEVQIAVEGREAYLTCGCGVLFAVDAVGGTIRWAVRYQRDGQPNVTM
                     RNYGYGQSMLDLSGWDDDVVIPHGQYLIVMSSDCDQLLAVDRRTGSFFWNSPRTSPFG
                     SVANYCLGVQGGRLFVGGKNVVRCYDLKGQGKLAWEKSIEDSFGRGCVTEDAIYVPVK
                     DSILKLDPQKGRELAQVGVALTSDEPVGNLFSDGEKLWVVGAGRVYAMTTLEHRMKML
                     EEQIAAGDGEAQLNRMRLHFKQNRAELALADLRGAYVLFQARETPDEAALRLFAAVNE
                     MKLTQKQPLVALQLLTELFVTATALPQLTKDALGRRADLVASAVSVIRQEKTRGALAP
                     LLAVAPLLTEEYLLSAAMFAVDGVATKDDAPALLEALKNGTPVAQLLSIRALTRLVPD
                     DAKAPLAERLPAGDDRVRLAAARALANLGERKDVLETFVALMESESPRVRNRSHQSLR
                     SLTGQQVNFVPEGKAEDRAAAIAQWKQWLAASGATAKLNLPLSDQAVPLGRTLFVSQG
                     QGLAVELDSDNKERWRQNLPGPAWGCQGLPNGHRLIAVYSHSMVVEYDDSGKEVWKKD
                     RLPGPPYTVQRLDNGNTLVACADVNQLVEISPDGTTTIVNVPGRPMSAQRLESGNTLV
                     ALQQGNRVVEVDRTGKIVWEARTSNPPGHAVRLDNGNTLVALTYGRQIVEFDPTGKTI
                     VWKTSVALTNPYAAQRLPSGNTLVADHQGIHEIEASGQKVRRMFRQQQVTGLSSY"
     gene            2564..>3752
                     /locus_tag="VFB80_20350"
     CDS             2564..>3752
                     /locus_tag="VFB80_20350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN36197.1"
                     /translation="MNLRYGNLVLVALTAIALRGAAADEATVAAPPRPHVQRLAEYGL
                     APTTESLAGYLASLVPTPERRAALAALVAQLGDDDYPRREQAALQLQKQAGGAAELLE
                     AAVAGDDYEVRWRARKILDQTDREGRGLLNAVFAAVREEKLTGLCGPLLAALPLCKDE
                     SLRAGMRRALAATATPADAPLLRELLTRSDPQARILGLATLAQALGPAADQELLPLLN
                     DPAEIVQVAAARALANHGRRESLAPLVRLLDSGEAIVRAEAARSLRASTGQHFSYTAY
                     DAAPARSAAIARWKEWLAAHGDSATLIVPLRETAYDLGRLLVCDHSQNLLMEFDTSGR
                     KLWQQAVGQQPWACQGLPSGHRLVGNYNERSVVEYDAAGREVWRIESLPGGPTSVERL
                     DNGN"
BASE COUNT          622 a         1257 c         1336 g          537 t
ORIGIN      
        1 cgctggaggg gaagcgggtc ggggcggagt cgcggccagc gcagatggcg gcccgcggct
       61 ttcagtacgg cgtgacgccg cggcgctcgc gcggcaactg gttgaccgct tatcaggcca
      121 ccggcggcaa ggcgctctgg acccgctcgg cgagcgacga ggacaaagaa ggctcgaccg
      181 acattggctt cctcgccgcg ccgacgccct gcggctcgtt gctgctcgcc ccgattaccg
      241 acggcggcac gatctggctc ttgggcctgg agcgcggcac gggcaagacg ctctggcggt
      301 cgtacctctg cgatgagccg cagggcggag cgtcgccctg gtcggaggtg cagatcgccg
      361 tcgaaggccg cgaagcctat ctcacttgcg gctgcggcgt gctcttcgcc gtggatgcgg
      421 tcggcggcac gatccgttgg gcggtccgct atcagcgcga cggccagccg aatgtcacga
      481 tgcggaacta cggctacgga caaagcatgc tcgatctcag cggctgggac gacgacgtgg
      541 tcatcccgca cgggcaatac ctgatcgtca tgtcgagcga ctgcgatcag ctgctggcgg
      601 tcgatcgccg cacgggcagt ttcttctgga attcgccgcg gaccagcccc ttcggcagcg
      661 tcgccaacta ttgcctgggc gtgcagggcg ggcggctgtt cgtcggcggc aagaacgtcg
      721 tccgctgcta cgacctgaag ggccagggca agctggcttg ggagaagtcc atcgaagatt
      781 cgttcggccg cggctgcgtc accgaagacg cgatctacgt gcccgtaaaa gattcgattc
      841 tcaagctcga cccgcagaag ggccgcgagc tggcccaggt cggcgtcgcc ctgacgagcg
      901 acgagccagt gggcaacctc ttctccgacg gcgagaagct gtgggtggtc ggcgcgggcc
      961 gcgtctatgc catgaccacg ctcgaacacc gcatgaagat gctggaggag cagatcgccg
     1021 ccggcgacgg cgaggcccag ctcaaccgca tgcggctgca cttcaagcag aaccgggcgg
     1081 agctggccct ggcggacctg cgcggggcct atgtcctctt ccaggcgcgc gaaacgcccg
     1141 acgaggccgc cctgcggctg ttcgcggcgg ttaacgagat gaagctcacg cagaagcagc
     1201 cgctggtggc gctccagctg ttgacggagc tgttcgtgac ggcgacggcg ctgccgcagc
     1261 tcacgaaaga cgcgctcggg cggcgggcgg acctggtggc cagcgcggtg agcgtgatcc
     1321 ggcaggaaaa aacccgcggc gcacttgcgc cgctgctcgc cgtcgcgccg ctgctcacgg
     1381 aagagtattt gctgtcggcc gcgatgttcg ccgtcgatgg tgtggcgacg aaggacgacg
     1441 cgccggcgct gctcgaagca ttgaagaacg gtacacctgt cgcgcagcta ctttcgatcc
     1501 gggcgctgac gcggctggtt cccgacgatg cgaaggcccc gctggccgag agactgccgg
     1561 cgggcgacga ccgcgtgcgg ctggcggcgg cccgtgcgct ggcgaacctg ggcgagcgga
     1621 aggacgtgct ggagacgttc gtcgccctga tggaatcgga atcgccgcgg gttcgcaacc
     1681 gcagccatca aagcctccgc agcctgacgg ggcagcaagt caacttcgtg ccggaaggca
     1741 aggccgagga tcgcgccgcc gcgatcgccc agtggaaaca gtggctggcg gccagcgggg
     1801 cgacggcgaa gctcaacctg ccgctctcgg accaggcggt gccgctgggg cggacgctgt
     1861 tcgtctcgca ggggcagggc ctggcggtcg agctcgactc ggacaacaag gagcgctggc
     1921 ggcagaacct gcccgggccg gcttggggct gccaggggct gcccaacggc caccggctga
     1981 tcgccgtcta ttcgcacagc atggtcgtcg agtacgacga cagcgggaag gaagtctgga
     2041 agaaagatcg cctgcccggc ccgccctata ccgtgcagcg gctcgacaat ggcaacacgc
     2101 tcgtggcctg cgccgacgta aatcagctcg tcgagatctc gccggacggc acgacgacga
     2161 tcgtcaacgt gccggggcgg ccgatgtcgg cgcagcgcct ggaaagcggc aacacgctgg
     2221 tcgcgctcca gcagggcaat cgcgtggtcg aagtcgatcg cacgggcaaa atcgtttggg
     2281 aagcccgcac gagcaacccg ccgggccacg ccgtgcggct ggacaacggc aacacgctcg
     2341 tggccctgac ctacggccgg cagatcgtcg agttcgaccc gaccggcaag acgattgtct
     2401 ggaagaccag cgtcgccttg accaacccct atgccgcgca gcgattgccc agcggcaaca
     2461 cgctggtggc cgaccaccag ggcattcacg agatcgaagc ctccggacag aaagtccgcc
     2521 gcatgttccg ccagcaacaa gtgacgggac tgtcgagcta ttgatgaacc ttcgatatgg
     2581 caatctggtg ctcgttgcat tgacggcgat cgccctgcgc ggcgccgcgg ccgacgaggc
     2641 caccgttgcc gcgccgccgc ggccgcacgt ccagcggctg gccgagtatg gcctggcgcc
     2701 gacgaccgag tcgctggccg gctacctggc gtcgctcgtg ccgacgcccg agcgccgcgc
     2761 ggccttggcg gcgctggtgg cccagctggg cgatgacgat tacccgcgcc gcgagcaggc
     2821 cgcgctgcaa ttgcagaaac aggccggcgg cgcggccgag ctgctcgaag cggccgtcgc
     2881 aggcgacgac tacgaagtgc gctggcgggc ccgcaagatt ctggaccaga ccgaccgcga
     2941 aggccgcggc ctgttgaacg ccgtgtttgc cgccgttcgc gaggaaaaac tgaccggcct
     3001 gtgcgggccg ctgctcgccg cgctgccgct gtgcaaggac gagtcgctgc gggctggcat
     3061 gcgccgcgca ctggccgcga ctgccacgcc cgccgatgcg ccgctcttgc gcgagctgct
     3121 cacccgcagc gatccgcagg cgcggatcct gggcctggcc acgctggctc aagcgctggg
     3181 cccggcggcc gatcaggaat tgctgccgct actcaacgat ccggccgaaa tcgtgcaggt
     3241 cgccgcggct cgcgccctgg ccaatcacgg ccgccgcgag tcgctcgcgc cgctggtgcg
     3301 gctgctcgat tcgggcgaag cgatcgtccg cgccgaagcg gcccgcagcc tgcgcgccag
     3361 caccgggcag catttcagct acaccgccta cgacgccgcg cccgcgcgct cggcggcgat
     3421 cgccaggtgg aaggagtggc tggcggcaca cggcgattcg gcgacgctca tcgtcccgct
     3481 gcgcgaaacg gcgtacgacc tgggacgcct gctcgtctgt gaccattccc agaatctgct
     3541 gatggagttc gacaccagcg gccgcaagct ctggcagcaa gcggtcggac agcagccgtg
     3601 ggcctgccag ggattgccga gcggccatcg cctggtgggg aactacaacg agcggtcggt
     3661 cgtcgagtac gacgccgccg gccgcgaggt gtggcggatc gagagcctcc ccggcgggcc
     3721 gaccagcgtc gagcgactgg acaacggcaa ta
//
LOCUS       DASRJB010000770         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2479800, whole genome shotgun sequence.
ACCESSION   DASRJB010000770 DASRJB010000000
VERSION     DASRJB010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479800"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..651
                     /locus_tag="VFB80_20355"
     CDS             175..651
                     /locus_tag="VFB80_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36198.1"
                     /translation="MSRFEYPNVCCRCAVNEPTQTWGVQSHESKPGDGNSVVTITYTC
                     PVPVCARCHRGLSMLLACCWFVALSIGAVAGWFIYDWAIHRPHADTFPEFLQIGMPVL
                     LGGMIAWGCAWLLKAFFINYDFVHYDPDNQTMVFKNKQYQYVFDQLNHTSPGRTWG"
     gene            complement(659..1525)
                     /locus_tag="VFB80_20360"
     CDS             complement(659..1525)
                     /locus_tag="VFB80_20360"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN36199.1"
                     /translation="MDRIAIAVLRGVCQLPAYVAKETGLFEAEGIAARLEVLPTAWVV
                     PERLLRGEFQFAVMPWTRVATASSRGEDLVLVCGSGYEEAAIVVRQGLALDDVQSIAV
                     PQEGGIKDLTAHGLMQSLGWDDRQKLRLPSGDGAILALVGQGADAASMVEPYASMLEA
                     LGIGTIVRSTGDLWPGAPGCSLATTRRLIEAQPDLVRRTVAAFASGAEAVRRRPDSAA
                     AIGAAYIGVHADFIRRALARNQPRIDALFNDRAQEQILEVMQELGYVDSRPADYLELG
                     FLREVACAPAAA"
     gene            complement(1563..>2289)
                     /locus_tag="VFB80_20365"
     CDS             complement(1563..>2289)
                     /locus_tag="VFB80_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36200.1"
                     /translation="QIRVVGPIVAPDLRPPSARSDGLLINLGGCGGSYNIDYARFVTR
                     IVLASPLAAQFAGRVTILAGEQCIRALQAEQGAAIQLCSASHTAALARLDQAELVLTA
                     PGLTATLESFQRCKPTFFLPPQNYSQWCILRALRGAGLAPAALHWEDLPEVAPLAERL
                     PEEVRNPLVRQALALGTADAAAAAAMEQCCARISEMDWAELAAWQHAFYASLGDNGAT
                     QIAADLAGRLSRARSAPAAAAPI"
BASE COUNT          375 a          784 c          777 g          353 t
ORIGIN      
        1 cggccgacgc cttggcagca acttcggcgg cctattccgc cgcgggagct ggcgaaaagc
       61 tcaccatcaa atgagcgccc tcccttagcg atcgtaacga atctgccgga agcagcgggc
      121 aggcagtgac ctagacttgc gcgaatccac aggccaatcg cgcgaggaca ggccatgagc
      181 cgctttgagt atccgaacgt ctgctgccgc tgcgcagtaa acgaaccgac gcagacctgg
      241 ggcgtgcaat cgcacgagtc caagcccggc gatggcaatt ccgtcgtgac catcacctac
      301 acctgcccgg tgccggtctg cgcgcggtgc caccgcggcc tgtcgatgtt gctcgcctgc
      361 tgctggttcg tagccctgtc gatcggcgcg gttgccggct ggttcatcta cgactgggcg
      421 atccaccggc cgcacgccga cacctttccc gagttcctgc aaatcggcat gcccgtgctg
      481 ctgggaggca tgatcgcctg gggctgcgcc tggctgctca aggccttctt catcaattac
      541 gacttcgtgc actacgaccc ggacaaccag acgatggtct tcaagaacaa gcagtaccag
      601 tacgtcttcg accagctgaa ccacacctcg ccgggccgca cgtggggcta ggcatgggct
      661 aagcggcggc cggagcgcag gcgacctcgc gcaagaatcc cagctccaga tagtccgccg
      721 gccgcgagtc cacgtagccg agctcctgca tcacctccag gatttgctcc tgcgcccggt
      781 cgttaaagag tgcgtcgatt cgcggctgat tccgcgccag ggcccggcga atgaagtcgg
      841 catgcacgcc gatgtaggcg gcgccgatcg ccgccgctga atcggggcgc cgacggacgg
      901 cttcggcgcc ggaagcaaac gccgcgacgg tgcggcgcac caggtcgggt tgcgcttcga
      961 tcaggcggcg cgtcgtcgcc agcgagcagc cgggcgcgcc gggccacaga tcgccggtgc
     1021 tgcggacgat cgtgccgatg ccgagggctt cgagcatgga ggcatacggc tcgaccatcg
     1081 aggccgcgtc ggcgccctgc ccgacgaggg ccaggatcgc gccgtcgccc gagggcaagc
     1141 ggagcttctg acggtcgtcc cagcccaggc tctgcatcag gccgtgggcg gtgaggtcct
     1201 tgattccgcc ctcctgcggc acggcaatcg attggacgtc gtccaaggcc aatccctggc
     1261 gcaccacgat cgccgcctcc tcgtagccgc tcccgcacac cagcaccagg tcctctcccc
     1321 ggctgctggc ggtcgccact cgcgtccagg gcatgaccgc gaactgaaat tcgccgcgca
     1381 acagccgctc gggcacaacc caagccgtcg gcagcacctc gagccgggcc gcgatgccct
     1441 cggcctcgaa gaggccggtt tctttggcga cgtaggcggg caactggcac acgccccgca
     1501 gcacggcaat ggcgatccga tccatgcatt cctcctaggt ggcgattcct cgaacgtgag
     1561 tgtcagatcg gcgcggcggc ggccggcgcg gagcgggcgc gactcaaccg accggccagg
     1621 tccgcggcga tttgcgtcgc gccattgtcg ccgagcgacg cataaaaggc gtgttgccag
     1681 gcggccagct cggcccagtc catttcagaa atccgggcgc agcactgctc cattgcggcc
     1741 gcggctgccg cgtcggccgt tcccagcgcg agcgcctggc ggacgagcgg attgcggact
     1801 tcctcgggca agcgctcggc gagcggcgcg acctctggca ggtcttccca atgcagcgct
     1861 gccggcgcga ggcccgcgcc tcgcagcgct cgcaggatgc accactggct gtagttctgc
     1921 ggcggcagaa agaaggtcgg cttgcagcgc tgaaagctct ccagcgtcgc ggtcaagccc
     1981 ggggcggtca ggactaattc ggcctggtcg aggcgcgcga gggcggccgt gtgcgaggcg
     2041 gagcagagct ggatggcggc gccctgctcg gcctgcaacg cgcggatgca ttgctcgccg
     2101 gccaggatcg tgacgcggcc agcgaattgg gcggccagcg ggctggccag aacgatccgg
     2161 gtgacgaagc gggcgtagtc gatgttgtag ctgccgccgc agccgccgag attgatgagc
     2221 agcccgtcgc tgcgagcgct cggcgggcgg aggtctggtg cgacgatcgg tcccacgacg
     2281 cggatctgc
//
LOCUS       DASRJB010000771        15124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_222586, whole genome shotgun sequence.
ACCESSION   DASRJB010000771 DASRJB010000000
VERSION     DASRJB010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222586"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1557)
                     /locus_tag="VFB80_20370"
     CDS             complement(346..1557)
                     /locus_tag="VFB80_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN36201.1"
                     /translation="MQNIIVEKPYEFIPPYRSTFWAELFRAIRIHRYYLRKSEGVVDY
                     EVRNVERLQASLAAGHGILITPNHPRTADPLAMGWLGVAARCNFWVMASWHLFQNSWL
                     YGWAIRAMGGFSVNREGVDRQSINMAIELLAGAQRPLVIFPEGATSRTADRLQALLDG
                     VAFIARAAAKKRSKQSPPGKVVVHPVAVKYYFRGNIRQVTDDVLTDIEHRLTWQPQRH
                     LPLLTRIGKVASGLLSLKELEHFNQPQTGTLAERLGRLMDRLLGPIETEWFGQVQHGQ
                     VVPRVRNLRCKILPDMVAGKTTQQERARRWQQLSDIYLAAQLATYPPDYLGRPTADRV
                     LEMVEKFEEDITDKARLHGDLKVVLDVGEAIEVSPERDRKASVDPLMTRIQESLQSMI
                     DKLAGDSPLLT"
     gene            complement(1637..2620)
                     /gene="galE"
                     /locus_tag="VFB80_20375"
     CDS             complement(1637..2620)
                     /gene="galE"
                     /locus_tag="VFB80_20375"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZN36202.1"
                     /translation="MRILVTGGAGYVGSHCLRHLLNGGHEAVVYDNLFQGHAPAVPAG
                     LLVQGELSDQPKLTALMKDRRIDAVMHFAALALVGESVQKPELYYRNNVLGSFHLLDA
                     MREAGVRKIVFSSTTATYGTPAKMPIAETTPQQPINPYGFSKLVVERMLDDFAAAHGF
                     GFAALRYFNAAGAAADGSIGEDHTPESHLIPIVLQVALGQRQAISIFGNDYPTPDGTC
                     VRDYVHVEDLATAHLAALERLQSGQAIKVNLGTGRGYSVREVIEACRRITGHPIPAVT
                     APRRAGDPPELIADARLARELLGWTPRYTTIESIVETAWRWHQSHPRGYRT"
     gene            2809..3756
                     /gene="xerD"
                     /locus_tag="VFB80_20380"
     CDS             2809..3756
                     /gene="xerD"
                     /locus_tag="VFB80_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZN36203.1"
                     /translation="MRPLKPKLIVPAAAAPGAGGQAARWIQSCLDYLRSECHLAANTI
                     AAYRRDLAHFSEWLSGRSAAKLTVRELSDYMGWLQTKKLAPPSVARHIVALKLFYRYL
                     QLEGVVRDNPAELLGTQKMWQRVPEVLSAALVEKLLSAPQPYDAYWRRDRALLELLYA
                     TGCRASEVSNLKLADLHLAERYCLAHGKGSKDRLTPLGDAAASAIKDYLAHERPELMH
                     GGRSDPGWLLLSRGGKRLRREAIWVLVKRYARRAGVSPDVSPHTLRHSFATHLLAGGA
                     DLRQVQELLGHASIATTQIYTHVDRSRLKRVHREFHPRA"
     gene            complement(3771..4829)
                     /locus_tag="VFB80_20385"
     CDS             complement(3771..4829)
                     /locus_tag="VFB80_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36204.1"
                     /translation="MIRKLLLVVLVGFATFACGLPAAVAQSQFDKVFPIKGQITQGII
                     TEMGRDKVKLDTSGLSRDFQVNEIRSITYADEPSELNSARNAAVRKSYSEALTDLKKL
                     DGMPPTRDVIRQDVEFYKALSLAKLAMTEGGDKAAASAAMLNFAKSAPQNFHFYEAAE
                     VMGDLAVASGNFADAVRFYGPVATAPWGDYQMRANNAIGRALAADKQFDPAIGKFDAV
                     LASDLATAEAVHQKQLATVGKSVCLAETGKADQGIAALQEIIAKNDPADAALFARTYN
                     ALGRCYLKQTKPKEALQAFLHTDLLFSAEPDAHAEALFYLSTLWNDMNKTERGLAART
                     TLQQRYAGSVWNNSNRKP"
     gene            complement(4855..8073)
                     /locus_tag="VFB80_20390"
     CDS             complement(4855..8073)
                     /locus_tag="VFB80_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36205.1"
                     /translation="MKRCRLPAAACRCLLLVGLFAGPAVCPAIEPARAFLDALREREL
                     FDVALDYLESAAKNPAVPISFKETLAYEKGVTLIQGAKLQRDTAIREKQLDEGQQALS
                     QFVNAQPTHLLAIATRIEMGKVIIERASIRVERAQKLSPAEKQKLHQEAHALYTEASK
                     VFGGLVEELKTKLKSYSAAIDETKEPKKVLERDRYRQDQIQAQMLAAATREEMAETLI
                     KDSKEWTETLTAALNGYKSAYEEYRTRLAGLHARIYQARCLQKLDRHKEASAILNELL
                     KNPDTPDAFHALKVKTMSLAVDSWLAQNLPLEILRKREQGQLPGPLELVAAARPAEER
                     SDEINAIRVAIAKAAKMYADELKRSKPNDPQIKLLLGDGRKHLAEVVKFPNPYQDEAR
                     KLLPEFAGGSVEAVAGRKEPKTFAEARTLAREAINDMQAGELMLGSLPALIAQAKSPE
                     RAELERRLADAEAQAQRGHADAMHYCKLALPLVREDTDIDEVNLIRYLICYLSFTEGN
                     YFDAVVIGDFLARRYPGSQHARSCAKIAMSGYLKLYAENSTDDKSVETSCLVEISDYI
                     VKTWPNQPEAAEALNTLIPFMIRSRQLAQAEDYLSRIPQDSPHRGNAELKTGQALWAS
                     YIDNSRQVRDWESDSQPRPEGLDLAARKAELEALKSKAKQILVDGVERMQGTGDVNAV
                     LVSAVLSLSQIYVDTNEAAKAVALLEDPKIGVLTLLQNDDPLLQREGFPEETYKTALR
                     AYISSLSAGGADANATIEKARGVMDALKQRIGQTPQGQARLVAIYISLARDLQQQMEI
                     ADPAAKKALGLGFENFLRQVGEDATELDILNWVAETYRGMGESYLTGQKGASAEAKAY
                     LRKAADTYVKLLDLGQKSPGFLTPAMTTQLRIQLAKTKKSLGDYIAARDTYESVLKAN
                     AMLLPVQVEAAHLYQDWGAAGKEHAPNYLKAMFGDRPDPRGKNVIWGWREIARMTASN
                     RQFREQFFESRYNLALCRYSYAQTIADAAKKKETLTASRRDITGLIELYPDLGEKWGP
                     QFDTLLKTIQKTLGEPATGLSGLRSAPAAPPANTTTSAAATK"
     gene            complement(8152..10296)
                     /locus_tag="VFB80_20395"
     CDS             complement(8152..10296)
                     /locus_tag="VFB80_20395"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HZN36206.1"
                     /translation="MSEPFHDAELDALLREVPLPDGFVQRLSEALAPAAERLDESLRA
                     VPVPSTMLARLKEIPADAELDASLADVAAPPALVSSLRRPTWPEQLWRASRQVTQATA
                     AASWFVALSLLLAAVGLLSTQHLFFSPVNDPLELVVTYDRPIPAAASAESAALESEVP
                     NPLAALDSQGKPGAPQIALIAPPDLGDPLRPGPVGQWVNVLRGGLRPLDDAVLLRYGL
                     LGAPHSADDRLPELVAPRLPRPTGIEPPPVRGYDRAFFLKHRVFPPIPPSANPKLAAL
                     DVPLVTFSDVLTRLERTLAEVRVPAAADLRTEELLAAMDYRLPAAASGKLAIRTAAGP
                     SLFGGPDVGLLQVAVQAGGLGMRPQTATHLVLAIDASHTMGVAGRLEMVKAAVSRLLD
                     QLTPRDRLSVVIFDEEIRHIVEALAPEDAASLRELLAGLAPRGGTNLAGGIQQAASLA
                     MNDAAGSAAARRLVLITDSEPLLPPETAERVEQVLADAAATGVQFDVLDVSYREQVDS
                     QLVQWATSLSGQARRIADGRQLYRALLESLAGRDPAIAHDARLTLRFNPKSVAAYRLI
                     GHEANALADITPASAEAAILAGESATALVELWFTPDDLDDLGVAELTWNDPATGAPAR
                     VRQRISRVQFAATAREMPLPLVQAALAAQVGEVLHGSHDVLRQAGLRPASSRGLAGVL
                     EAAKEANPRLHERADLERLLRLAEQLQKQGVK"
     gene            complement(10305..10802)
                     /locus_tag="VFB80_20400"
     CDS             complement(10305..10802)
                     /locus_tag="VFB80_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN36207.1"
                     /translation="MALTLAAFNTLVDEHGPALYRIAYRLVGDRHEAEDVVQETYRSA
                     WKSRESYESGRGDRAWLAAILRRRVIDRLRRHAPPHPFDAAGPHEVGVEGADPFAGQY
                     TDEMQHALAQLPEELRETLLLVVVGELTHQEVADLLGAPLGTVLSRVSRARRRLREVL
                     ISART"
     gene            11095..13257
                     /locus_tag="VFB80_20405"
     CDS             11095..13257
                     /locus_tag="VFB80_20405"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN36208.1"
                     /translation="MQGRKRSHSPAAIEIDFQSLLPRGAAFDPSTLEVEVLGSTRDAD
                     RRLVPHRLDPSFGSSQAKLNFVVPDETCTRFAVYFDTADGRRGQPRRYPGLLGDGDRF
                     CERFGQREITASHFDQFVDFDGDGDLDLFKGGVEPYVSCWENVGGNRLLDWGRLASDG
                     KLLALPASRDNRSWLTVAFFDIDGDGDQDFFPSFGDGPDAGQIVFYRNTTAGRGAPLS
                     FARVGPLQTESGRLLAGGEQAGGWFPSIALVRDWDGSAEGRIDAIVGSNHRCWLYRGR
                     GVDDRNAPIFAAAEAIQADGVPIHLINPRFYPVDIDADGDLDLPAGSQPGPIWLFENR
                     GTRKQPLLAAGQVIGLSGKYLIGDAHSGLSIADFDGDGLLDLLAGRFWERTDLNDPTR
                     LRDFGGFYRNTGTRSAPRFQRADRGGPFTEQFQICDAVRQNCVRAVDWDKGGKLDLLA
                     GDTDGFIWFFGNEASQSAPLFAGGVRVRAGGQPLSVSKSGGHARFDICDWNADGTDDL
                     LVADGQGTLTLFRGQAGPPGGELSAGEAVLAAGQPIRGAARASVLVCDWNQDGLFDVV
                     FADERGYRWHRNTGEKGRPALAAAQPLDFGGGTVKYVRPNLGSFVDWDGDGRQDFIGC
                     NFENSIRLYRNVAEAGRGAEPRFADAEGLTILQASSPQMISGAHAIDWNGDGDLDLLT
                     GQGHGGSGLRFFERDWIEDELHGTHPRVTAGKIETKPN"
     gene            complement(13254..14459)
                     /locus_tag="VFB80_20410"
     CDS             complement(13254..14459)
                     /locus_tag="VFB80_20410"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN36209.1"
                     /translation="MGDNKINRRAVLAAGTGGAIGMLGQGKLDAAPVAGNASVYEQLG
                     VQPIINAAGTITMLGGSLMPPEVIAAWTAASKQFVPLLELQDRVGERIAQRLGVEAAL
                     VTTGAAGAMLVGTAACVTYKDRSLIGQLPLPTEKGVEVIRQKSHRDCYDQQVTACGVK
                     LVDVETAGDLEQAINPRTAMLLAYNFLEPHGKIRHAEWLAAARRHGIPTLLDAAADTP
                     PRERLSEYNKLGYDLVAFSGGKAIRGPQDAGLLLGRKDLIEAAKLNTSPRCSIGRGLK
                     VSKEDMVAMWAAIERFMQLDHAADDREWRRRIDAIIAALKDVPTIRAETIVPAIENHV
                     PHLLLHWDEEKLKVTPQQMKTRLAAGNPPIATARVHGTGDTGLLISVFMLQPGEETIV
                     GERIRAILS"
     gene            complement(14472..>15124)
                     /locus_tag="VFB80_20415"
     CDS             complement(14472..>15124)
                     /locus_tag="VFB80_20415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HZN36210.1"
                     /translation="FVFYALFLALGFAPRNRGYQTPTADAVVIFVRSNEVHTDVVLPV
                     TSETGVDWRQLFPPQHFRRDVRAAKYVAVGWGNREFYVNTPTWSEFRLSTAVGALFWP
                     SESVLHVEYLADAAEGQWFREVRLSREQYQQLAAFVRSSAGSVDERGCVKTASDRSYT
                     SSDRFYCAAGRYHCFNTCNQWTGRGLAKAGVPVGIWTPLQPQVLYWLPQVRPDADQ"
BASE COUNT         2402 a         4942 c         5076 g         2704 t
ORIGIN      
        1 ctttgtgttt catgctccgc ccgtgtctcg cacgagttgt ctttcgcgct tcgtagtact
       61 gagtgcaaag ttcggagtca gagtccggtg cagaatcctg gacaacggac gtctcactcc
      121 attcgagtcc tgcgaaagac ccggtaaatc gaattgattg ccaaataaat tgccacaaac
      181 cccatgatcg ccgtttttgg caataaattc accggatttc agaggctgcc aaaaatcgca
      241 acttctgaag taccaaggac ttacgagttg gtggctaggg cgaaaacggt cgaataagtt
      301 gccacgaggc cccaccccaa aacctggcaa ctaattcggc gccggtcagg tgagcagcgg
      361 cgagtcgccg gcgagcttgt cgatcatgct ttgcagcgac tcctggatcc gcgtcatcag
      421 cggatcgacg gaggccttgc ggtcgcgctc ggggctgacc tcgatcgctt cgccgacgtc
      481 gagcacgacc ttgaggtcgc cgtgcaggcg agccttgtcg gtgatgtcct cctcgaactt
      541 ctcgaccatc tccagcacgc gatcggccgt cggccggccc aggtaatccg gcggataggt
      601 ggccagctgc gcggccaggt agatgtcgga cagctgctgc cagcggcggg cccgctcctg
      661 ctgcgtcgtc ttgccggcga ccatgtcggg cagaatcttg caacgcaggt tgcggacccg
      721 cggcaccacc tgcccgtgtt gcacctggcc gaaccactcg gtctcgatcg gacccagcaa
      781 ccggtccatc aggcggccca gccgctcggc cagcgtgccg gtttgcggct gattgaagtg
      841 ctccagctcc ttgaggctca gcaggcccga cgcgaccttg ccgatccgcg tcaggagcgg
      901 caagtgccgc tgcggctgcc aggtgaggcg gtgctcaatg tcggtgagca cgtcgtcggt
      961 cacctggcgg atgttgccac ggaaatagta cttcaccgcg accggatgca cgacgacttt
     1021 gcccggcggc gattgcttgg agcgcttctt cgccgctgcg cgggcgatga aggccactcc
     1081 gtcgagcagc gcctgcaggc gatcggcggt ccggctggtc gcgccttcgg gaaaaatgac
     1141 cagcggccgc tgcgccccgg ccagcagctc gatggccatg ttgatcgact gccgatcgac
     1201 gccctcgcgg ttgacgctga agccgcccat cgctcggatc gcccagccat agagccagct
     1261 gttctggaag aggtgccagc tggccatgac ccagaagttg cagcgagccg cgacgcccag
     1321 ccagcccatg gccagcgggt cggccgtgcg gggatggttg ggcgtgatca ggatgccgtg
     1381 gcccgcggcc agcgacgcct ggaggcgctc gacattccgc acttcatagt ccaccacgcc
     1441 ttccgatttg cggaggtaat agcggtgaat acggatcgcg cgaaacagct ccgcccagaa
     1501 ggtgctgcga tagggcggga tgaactcgta gggcttctcg acgatgatgt tctgcatgcc
     1561 gagggctcgg ggctgtgggt tcggggttcg gcaggagcag tgtatcttct gaaccctgaa
     1621 ctccgaaccc tgaaccctat gtccgatagc cccgcgggtg cgactggtgc cagcgccagg
     1681 cggtttcgac gatcgattcg atcgtggtgt agcggggcgt ccagccgagc agctcgcggg
     1741 ccaggcgggc gtcggcgatc agctcgggcg gatcgcccgc tcggcgcggg gcggtgacgg
     1801 cgggaatcgg atggcccgta atccggcggc aggcttcgat tacctcgcgg acgctgtagc
     1861 cgcggcccgt gccgaggttc actttgatcg cctggcccga ctgcaagcgc tccagcgcgg
     1921 cgaggtgagc cgtcgccagg tcctcgacgt ggacgtagtc gcggacgcag gtgccgtcgg
     1981 gtgtgggata gtcgttgccg aaaatgctga tcgcttgacg ctggccgagg gcgacctgca
     2041 gcacgatcgg gatcaggtgc gactcgggcg tgtggtcctc gccgatcgag ccgtcggcgg
     2101 ccgcgccggc ggcgttgaag tatcgcaggg ccgcgaaacc gaagccgtgg gccgcggcaa
     2161 agtcgtccag catccgctcg accaccagct tcgagaagcc gtaagggttg atcggctgct
     2221 ggggcgtggt ctcggcgatc ggcatcttgg ccggcgtgcc gtaagtcgcg gtggtgctcg
     2281 agaagacgat cttccgcacg ccggcctcgc gcatcgcatc gagcaggtgg aacgagccca
     2341 gcacgttgtt ccggtagtag agctcgggct tctgcaccga ttcgccgacg agggccagcg
     2401 ccgcgaagtg catgaccgcg tcgatgcggc ggtccttcat gagcgccgtc agcttcggct
     2461 ggtcgctcaa ctcgccctgc accaataggc ccgcggggac tgccggcgcg tggccctgaa
     2521 agaggttgtc gtagacgacg gcctcgtggc cgccatttag caggtgccgc aggcaatgcg
     2581 acccgacata gcccgcgccg ccggtgacca gaatccgcat gattggctcc aacaagtggc
     2641 gagcgctgtg tttcttgacc gcgggcttcg ctgagtttct taacggcggg cttcgctgag
     2701 tctaccggct ctagggctta ttaccaatgc ggcggcatcc atcggccgct tcagcgtgtc
     2761 gtggatgcca gccgatgaca gcgatacgcc acactccgcg agctgccgat gcgcccgctc
     2821 aagcccaagc tgatcgtccc ggccgctgcc gcgccaggcg ccggcggcca ggcggcgcgg
     2881 tggatccagt cgtgcctcga ctacttgcga agcgagtgcc acctggcggc caacacgatc
     2941 gccgcgtatc gccgcgacct ggcacacttc agcgagtggc taagcggccg cagcgccgcg
     3001 aagctgaccg tccgcgagct gtcggactac atgggctggc tgcagacgaa gaagctcgca
     3061 ccgccgagcg tcgcgcggca catcgtggcc ctcaagctgt tctaccggta cctgcagctc
     3121 gagggcgtcg ttcgcgacaa cccggccgag ctactgggca cgcagaaaat gtggcaacgc
     3181 gtgccggagg tgctctccgc ggccctcgta gagaagctcc tttccgctcc gcagccctac
     3241 gatgcctact ggcggcgcga ccgagcactg ctcgagctgc tctacgccac gggctgccgg
     3301 gcgtcggaag tgtcgaacct gaagctggcg gatttgcacc tggccgagcg gtactgcctg
     3361 gcgcacggga agggatcgaa ggaccgcctg acgccgctgg gcgacgcggc ggccagcgcg
     3421 atcaaggatt acctggcaca cgagcgaccg gagctgatgc acggcggccg cagcgatccg
     3481 gggtggctgt tgctctcgcg cggcggcaag cggctgcggc gggaggcgat ttgggtcctg
     3541 gtgaagcggt atgcccgccg ggccggcgtc agtccggacg tcagcccgca cacgctgcgg
     3601 cacagctttg ccacgcatct attggcaggc ggggccgacc tgcggcaggt gcaggaattg
     3661 ctcggccacg ccagcatcgc cacgacgcag atctacaccc acgtcgatcg ctcgcggcta
     3721 aagcgcgtgc accgggagtt ccatccgcgg gcgtagtcgg cgggcggcgg ctacggtttg
     3781 cggttcgagt tgttccacac gctgccggcg tagcgctgct gcagcgtggt ccgcgcggcg
     3841 agcccccgct cggtcttgtt catgtcgttc cagagtgtgc tgagatagaa cagggcctcg
     3901 gcgtgagcgt cgggctcggc gctgaacagc aggtcggtgt gcaggaacgc ctgcagggcc
     3961 tccttcggct tggtctgctt cagatagcac cggccgagag cgttgtacgt ccgggcgaag
     4021 agggccgcgt cggccggatc gttcttggcg atgatctcct ggagcgcggc gatgccctga
     4081 tcggccttgc cggtctcggc cagacagacg gacttgccta cggtggcgag ctgcttttga
     4141 tggacggctt cggcggtggc caggtcgctg gccagcacgg cgtcgaactt gccgatggcc
     4201 gggtcgaact gcttgtcggc ggccagggcc cggccgatgg cgttgttggc ccgcatctgg
     4261 taatcgcccc agggggcggt ggcgaccggc ccgtagaagc gcacggcgtc ggcaaagttg
     4321 cccgaggcca ccgccagatc gcccatcacc tcggcggctt cgtagaagtg gaagttctgc
     4381 ggggcgctct tggcgaagtt cagcatcgcc gcactggcgg ccgccttgtc cccgccttcg
     4441 gtcatcgcca gcttcgccag cgagagcgcc ttgtagaact cgacgtcctg ccggatcacg
     4501 tcgcgggtcg gcggcatgcc gtcgagcttc ttcaggtcgg tcagcgcttc gctatagctc
     4561 ttccgcacgg cggcgttgcg ggcgctattc agctcgctcg gctcgtcggc ataggtgatc
     4621 gagcgaatct cgttgacttg aaagtcgcgg ctgagcccgc tcgtgtcgag cttgaccttg
     4681 tcgcggccca tctcggtgat gatgccctgc gtgatctgcc ccttgatggg aaaaaccttg
     4741 tcgaactggc tttgagccac cgccgcgggc aatccgcacg cgaaggtcgc gaagccaacg
     4801 agtacaacga gcaataactt gcgaatcatg gtcactccga tagttggatg ccgctcactt
     4861 cgtggcggcg gcgctggtgg tcgtgttggc cggcggtgcg gccggggccg atcgcagtcc
     4921 ggacaagccg gtggcaggct cgccgagggt cttctgaatc gtcttgagca gcgtgtcgaa
     4981 ctgcggcccc catttctcgc ccaggtcggg atacagctcg atcaggccgg tgatgtcgcg
     5041 gcgcgacgcc gtcagtgtct cctttttctt tgccgcatcg gcgatcgtct gggcatagct
     5101 gtagcggcac agcgccaggt tatagcggga ctcgaagaac tgctcgcgga actggcggtt
     5161 gctggcggtc atgcgggcga tttcgcgcca gccccaaatg acgttcttgc cccgtggatc
     5221 cggacgatcg ccaaacatgg ccttgaggta gttcggggca tgctccttgc cggccgcgcc
     5281 ccaatcctgg tacaggtggg cggcctccac ctgcaccggc agcaacatgg cgttggcctt
     5341 gagcaccgat tcgtaggtat cgcgggcggc gatgtagtcc cccaggctct tcttggtctt
     5401 ggccagctga atccgcagct gcgtggtcat ggccggcgtg aggaagcccg gcgacttctg
     5461 gcccaggtcg agcagcttga cgtaggtgtc ggccgccttg cggagatagg ccttggcctc
     5521 ggccgaggcg cccttctggc ccgtgagata gctttcgccc atgccgcgat aggtctcggc
     5581 gacccagttg aggatatcca gctcggtcgc gtcctcgccg acttgccgca ggaagttctc
     5641 gaagcccagc ccgagcgcct tcttcgcggc gggatcggcg atctccatct gctgctgcaa
     5701 gtcgcgggcg aggctgatgt agattgccac cagccgggcc tggccctgcg gcgtctggcc
     5761 gatccgctgc ttgagggcgt ccatcacgcc gcgggctttt tcaatcgtcg cattcgcatc
     5821 cgcgccgccc gcggaaagcg acgagatata cgctcgcagc gccgtcttgt acgtctcttc
     5881 ggggaagcct tccctttgca gcagcggatc gtcattttgc agcagcgtca gcacgccgat
     5941 cttcgggtct tccaacagcg cgacggcctt ggccgcttcg ttcgtatcga cgtaaatctg
     6001 cgacagcgac agcacggccg agaccagcac cgcattcaca tcgcccgtgc cctgcatccg
     6061 ctcgacaccg tcgaccagga tctgtttggc cttggacttg agcgcttcga gctcggcctt
     6121 gcgggccgcc aggtccaggc cctccggccg gggctgggag tcgctctccc aatcgcggac
     6181 ctgccgcgag ttgtcgatgt agctggccca cagggcctgc cccgtcttca gctcggcgtt
     6241 gccgcgatgc ggcgaatcct gcggaattcg cgacaggtag tcctccgcct gggcaagctg
     6301 ccgcgagcgg atcatgaacg gaatcagcgt gttgagggcc tcggcggcct ccggctgatt
     6361 gggccacgtc ttcacgatgt agtcggagat ctccacgagg caggaagtct cgaccgattt
     6421 gtcgtcggtg gagttctcgg cgtacagctt caagtagccg ctcatcgcaa tcttggcgca
     6481 cgagcgggcg tgctggctgc cgggatagcg gcgggccaga aaatcgccga tgacgaccgc
     6541 gtcgaaataa ttgccttccg tgaacgacaa gtagcagatc aggtagcgga tcaggttgac
     6601 ctcgtcgatg tcggtgtcct cccggaccag cggcaaggcg agcttgcaat agtgcatcgc
     6661 gtcggcgtgc ccgcgctgcg cctgggcctc ggcgtcagcg agccgccttt ccagctcggc
     6721 acgttcgggc gacttggcct gggcaatgag cgctggcagc gaaccgagca tcagctcgcc
     6781 ggcctgcatg tcgttgatgg cctccctggc cagcgtgcgg gcctctgcga acgtcttggg
     6841 ctccttgcgg ccggccactg cctcgacgct gccgccggcg aattccggga gcagcttccg
     6901 agcctcgtcc tggtagggat tcggaaattt aacgacctcc gcgaggtgct tgcggccgtc
     6961 gccgagcagc aatttgatct gcgggtcgtt gggcttcgaa cgcttcagtt cgtccgcgta
     7021 catcttcgcc gccttggcga tggccacgcg gatggcgttg atctcgtcgc tccgctcctc
     7081 ggccggccgc gcggcggcga ccagctccag cggcccaggc agctggccct gctcgcgctt
     7141 tcgcaaaatc tccagcggca ggttttgcgc cagccaggaa tcgacggcca gcgacatcgt
     7201 cttgaccttc agcgcgtgga aggcgtcggg cgtatcgggg tttttcagca gttcgttgag
     7261 gatcgccgag gcctccttgt gccggtcgag cttctgcagg cagcgggcct ggtatatgcg
     7321 ggcatggagc cccgccagcc gcgtgcgata ctcctcatag gcggacttgt agccgttcag
     7381 cgccgccgtc agcgtttcgg tccactcctt ggagtccttg atcagcgtct cggccatttc
     7441 ttcgcgcgtg gccgccgcaa gcatctgcgc ctggatctgg tcctggcggt agcggtcgcg
     7501 ctccaagacc ttcttcggtt ccttggtctc gtcgatcgcg gccgaatacg acttgagctt
     7561 cgtcttcagc tcttccacga gcccgccgaa taccttgctg gcttcggtgt agagcgcgtg
     7621 ggcctcttgg tgcagcttct gcttctcggc cggcgacagc ttttgcgctc gctcgacgcg
     7681 gatgctggcc cgctcgatga tcactttgcc catctcgatc cgcgtggcaa tggcgagcag
     7741 gtgcgtgggc tgcgcgttga cgaactggga cagcgcctgc tggccttcgt cgagctgctt
     7801 ctcgcgaatg gcggtgtcgc gctgcaactt cgcaccctgg atcagcgtta cgcccttctc
     7861 gtaggccagc gtctccttga agctgatcgg caccgccggg ttcttcgccg ccgactcgag
     7921 atagtcgagc gcgacgtcga acagctcccg ctcgcgcaag gcgtccaaga aggcccgcgc
     7981 cggctcgatc gcagggcaaa ccgccggacc agcaaacagc cccaccagca gcaggcatcg
     8041 gcaagccgcc gcaggcagcc ggcatcgctt catgctcagt ctccactacc tcaacaatcg
     8101 ctgtgcccga ttatagctcg cgcaaggcgg ccgcaactcc gcacgatgca gctatttgac
     8161 gccttgtttc tgcagctgct cggccagccg cagcagccgc tccaggtcgg cccgttcgtg
     8221 caagcgggga ttggcctcct tcgcggcctc gagcacgccg gccagccccc ggctgcttgc
     8281 cggccgcagg cccgcctggc gcaatacgtc gtgcgagccg tggagcactt ctccgacctg
     8341 cgccgccagc gctgcctgaa ccagcggcag cggcatctcg cgagcggtag ccgcgaactg
     8401 cacgcgactg atgcgctgcc gcacgcgagc gggcgcgccg gttgccggat cgttccaagt
     8461 caactcggcg acgcccagat cgtcgagatc gtcgggagtg aaccacagct cgacgagtgc
     8521 tgtagccgat tcgcctgcca ggattgccgc ttcggccgag gcgggcgtga tgtcggccag
     8581 ggcgtttgcc tcgtgcccga tcaggcggta ggccgcaaca gatttcgggt tgaaccgcaa
     8641 ggtcaggcgg gcatcgtgag cgatggccgg atcgcgtccg gcgagagatt cgagcagtgc
     8701 ccggtagagt tgccggccgt cggcaatgcg gcgggcctgg cccgacaggc tggtcgccca
     8761 ctgcacgagc tgcgaatcga cctgctcgcg atacgacacg tcgagcacgt cgaactgaac
     8821 gcccgtcgcg gctgcgtccg cgagcacttg ctcgacccgc tcggccgttt ccggtggcaa
     8881 cagaggctcg ctgtcggtga tcagcaccag ccggcgggcg gcggcgctcc ccgcggcgtc
     8941 gttcatcgcc agcgaggccg cctgctgaat accgccggcc agattcgttc cgccgcgcgg
     9001 agcaaggccg gcgagcaatt cgcggaggga agctgcgtct tcgggcgcga gagcttcgac
     9061 gatgtggcgg atttcctcgt cgaagatcac caccgacaag cggtcgcgcg gcgtcagctg
     9121 gtcgagcagc cggctgactg ccgccttgac catctccaac cggccggcga cgcccatcgt
     9181 gtgcgaggcg tcgatggcca gcaccaggtg cgtcgccgtt tgtgggcgca tgccgaggcc
     9241 gcccgcctgc accgcgactt gcagcaagcc gacatcgggc ccgccgaaca gcgaagggcc
     9301 ggccgccgtg cgaatggcca gcttgcccga agcagccgcc ggcaagcggt agtccatcgc
     9361 ggccagcagc tcttcggtgc gcagatcggc agccgcgggc acgcggactt cggccagcgt
     9421 ccgctccagc cgcgtcagca cgtcgctgaa cgtgaccagg ggaacatcga gcgctgccag
     9481 cttcgggttg gcgcttggcg gaatcggcgg gaagacgcga tgcttcaaga agaacgcccg
     9541 gtcgtagccg cggaccggcg gtggctcgat acccgtcggc cgcggcagcc gcggagctac
     9601 cagctccggc agccggtcgt cggccgagtg cggcgcaccg agcaagccgt agcgcagcaa
     9661 cacggcgtcg tcgagcggcc gcagcccgcc gcgcagcacg ttgacccact ggcccacagg
     9721 cccgggacgc agcggatcgc caagatccgg cggggcgatg agcgcgatct ggggcgcgcc
     9781 tggttttccc tgtgaatcaa gggccgcaag cggattggga acttcgctct ccaaggcggc
     9841 agattccgcg gacgctgcgg ccgggatcgg gcggtcgtag gtcacgacca gctccagcgg
     9901 gtcattgacg ggcgagaaga acaggtgctg cgtggacaac aggccgacgg cggccagcag
     9961 cagcgacagc gcgacgaacc acgaggccgc cgcggtcgcc tgcgtcacct gccgactcgc
    10021 gcgccagagc tgctctggcc aggtcggtcg ccgcaaactc gaaaccaggg ccggcggcgc
    10081 cgctacatcg gccagcgagg cgtccaattc agcatccgcg ggaatctcct tcagccgcgc
    10141 cagcatcgtg ctgggcaccg gcacggcccg cagcgattcg tccaggcgct cggccgccgg
    10201 cgcgagcgct tcggagagcc gctggacaaa gccgtccggc agcggcacct cgcggagcag
    10261 cgcatcgagc tcggcatcgt ggaagggttc ggacatggac aatttcacgt tcgggcggag
    10321 atcagcacct cgcgcagccg ccgccgcgcc cggctgacgc gggacagcac ggtgccgagc
    10381 ggggcgccga gcaggtcggc gacctcctgg tgggtcaact cacccacaac gaccagcagc
    10441 agcgtttcgc gcagctcctc aggcagctgg gcgagggcgt gctgcatctc gtcggtatat
    10501 tggcccgcaa aggggtcggc cccctcgacg ccgacttcgt gcggaccggc tgcgtcgaag
    10561 ggatggggcg gggcgtggcg gcgcaagcgg tcgatcacgc ggcggcgcag aatcgccgcc
    10621 agccaagcgc ggtcgccgcg cccgctctcg tacgattcgc gactcttcca ggccgagcga
    10681 taggtctcct ggaccacatc ctcggcctca tgccgatccc ccacgaggcg gtaagcaatc
    10741 cggtacaaag ccggaccatg ctcgtccacc agcgtgttga acgcggctaa ggtcaatgcc
    10801 aaagcctgac ctccgctgca tggccaccag caaagccgca tctcaggcct ttgtggacgc
    10861 ggcttcacta accagattcg tcccctgcca gccggagtat tcccggccga gcatttattc
    10921 tatctcattg cgcggccatg tgttacgttc gcagtttcta tgatcgcgag gaatgtcgcc
    10981 ccagcacttt gcatcgtggc gtggctcacg gccccgccag cgcggtcaca ggaaccagat
    11041 agcagtccgc gttggacctc gccgaatcga tatcgacttc tcctttcggt cgagatgcag
    11101 gggcgcaagc gctcccattc gccggcggca attgaaatcg attttcaatc gctgctgccg
    11161 cggggcgcgg cgttcgaccc atctacactc gaggtcgaag tgctcggatc gacgcgcgac
    11221 gccgatcgcc ggctcgtgcc gcatcggctc gatccgagct tcggcagctc gcaagcgaaa
    11281 ttgaacttcg tggtgccgga cgagacttgc actcgcttcg ccgtttattt tgacactgcc
    11341 gatgggcggc gcggtcagcc gcggcggtat ccgggactgc taggggacgg cgatcggttc
    11401 tgcgaacgtt ttggtcagcg cgagatcacg gcgagccact tcgaccagtt tgttgatttt
    11461 gacggcgacg gcgatctcga tttgttcaag ggcggagtcg aaccgtacgt ctcttgctgg
    11521 gagaacgtcg gcggcaaccg gctgttggat tggggaaggc tcgcgtcgga tggaaaattg
    11581 ctggccctgc ctgctagccg cgacaaccgc agctggctga ccgtcgcgtt tttcgacatc
    11641 gatggcgacg gcgatcagga tttctttccc agctttggcg acggtcccga tgccgggcag
    11701 atcgtgttct atcggaacac gaccgcggga cgcggcgcgc cgctgagctt cgcccgcgtt
    11761 ggtccgctgc aaacggagtc cggccggcta ttggctggcg gcgaacaggc tggcggctgg
    11821 tttccgtcga ttgcgttggt ccgcgattgg gacggctcgg cagagggccg catcgatgcg
    11881 attgtcggca gcaatcatcg ctgctggctc tatcgtggcc gcggcgtcga tgaccgcaac
    11941 gcgccgattt tcgccgcagc cgaggccatc caagcggacg gcgtcccgat ccacctgatc
    12001 aatccgcggt tctatccggt cgatatcgac gccgacggcg acttggactt gccggcgggc
    12061 tcgcagcccg ggccgatctg gctatttgag aatcggggca cacgcaagca gcctctactt
    12121 gccgcggggc aagtcatcgg gctcagcggc aaatatctga tcggcgacgc tcacagcgga
    12181 ttgtccatcg ccgacttcga cggggacggc ctgctcgatt tgctggcggg ccgcttttgg
    12241 gagcggaccg acctgaatga tccgactcgg ctgcgcgact tcggcgggtt ctaccgcaac
    12301 actggcacgc ggagtgcgcc gcgcttccag cgggccgatc gcggcgggcc gttcaccgag
    12361 caatttcaga tctgcgacgc cgtgcggcaa aactgtgttc gtgccgtaga ttgggacaag
    12421 ggcggcaagc tcgacctcct ggccggcgac acggacggct tcatctggtt ctttggcaac
    12481 gaggcaagcc agtccgcgcc gctgtttgcc ggcggcgtga gggttcgcgc gggcggccag
    12541 ccgctctcgg tctccaagag cggcggtcac gcccgcttcg atatttgtga ttggaacgcc
    12601 gacggtaccg acgacctgct cgtcgccgac gggcaaggga cgctgacgct cttccgcggc
    12661 caggccgggc cgcctggcgg cgagttatcg gcgggtgagg cggtgctcgc cgccggacag
    12721 ccgattcgag gcgcagcgcg ggccagcgtg ctcgtctgcg actggaatca agacggcctg
    12781 ttcgacgttg tattcgccga cgagcgcggc taccgctggc atcggaacac cggcgaaaaa
    12841 ggccgacccg cgcttgccgc cgcgcagccg ctcgactttg gcggcggcac cgtgaaatac
    12901 gtccggccga atctgggctc gttcgtcgat tgggacggcg acggccggca ggacttcatc
    12961 ggctgcaact tcgaaaacag catccgcctg tatcgcaacg tggccgaggc tggccgcggc
    13021 gcggagccga gatttgccga tgccgaaggc cttacgatcc tgcaggcctc ttcgccacag
    13081 atgatctccg gcgcgcacgc gatcgattgg aacggcgacg gcgacctcga cttactgacc
    13141 gggcaggggc acggcggcag cggcctgcga ttcttcgagc gggactggat cgaagacgag
    13201 ctgcacggga cgcatccccg tgtgacagcg ggcaaaatcg agacgaagcc caactaagac
    13261 aggatcgccc ggatccgctc gccgacgatc gtttcctcgc cgggctggag catgaagacg
    13321 gagatcagaa ggccggtgtc gcccgtgccg tgaacccggg cggtggcgat gggcggattg
    13381 cccgcggcca gtcgggtttt catctgttgc ggcgtgacct tgagcttctc ttcgtcccag
    13441 tgcaagagca ggtgcggcac gtggttctcg atggcgggca cgatcgtctc ggcccggatg
    13501 gtcggcacgt ccttgagcgc ggcgatgatc gcgtcgatcc gccgccgcca ttcgcggtcg
    13561 tcggctgcgt gatcgagctg catgaagcgc tcgattgcgg cccacatcgc gaccatgtct
    13621 tccttgctga ctttcaggcc gcggccgatg ctgcaccgcg gcgacgtatt gagcttggcc
    13681 gcctcgatca ggtccttgcg ccccaggagc aggccggcgt cctgcgggcc gcggatcgcc
    13741 ttgccgccgc tgaaggccac caggtcgtag ccgagcttgt tgtactccga cagccgctcg
    13801 cgcggcggcg tgtcggccgc ggcgtcgagc agcgtcggaa tgccgtgtct ccgcgccgcc
    13861 gcgagccact cggcatgccg aatcttgccg tgcggctcga gaaagttata ggccagcagc
    13921 atggccgtcc gcgggttgat ggcctgctcc aggtcgcccg cggtttccac atcgacgagc
    13981 ttcacgccgc aagccgtcac ctgctggtcg tagcagtcgc ggtgcgattt ttggcgaatg
    14041 acttcgacgc ccttctcggt tggcaggggc aactggccga tgaggctgcg gtccttgtag
    14101 gtgacgcagg cagccgtgcc gacgagcatc gcacccgccg cacccgtcgt taccagcgcc
    14161 gcctcgacgc ccaatcgctg ggcgatccgc tcgccgacgc ggtcctgcag ctccagcagt
    14221 gggacgaact gtttcgaggc ggccgtccag gcggcgatca cctccggcgg catcagcgag
    14281 ccgcccagca tcgtgatcgt gccggccgcg ttgatgatcg gctgcacgcc gagctgttcg
    14341 taaacgctgg cattgccggc caccggcgcg gcatcaagtt ttccctgacc gagcatgcca
    14401 attgccccgc cggtacctgc cgccagcacg gcccgccgat tgatcttgtt gtcgcccatc
    14461 gccgcctccg gctactggtc tgcatcagga cggacttgcg gtagccaata tagcacctgc
    14521 ggctgaagcg gcgtccaaat gccgacggga acaccggcct tggccaggcc gcggccggtc
    14581 cattggttgc acgtgttgaa gcaatggtag cggccggcgg cgcagtaaaa gcggtcgctg
    14641 ctcgtgtagc tgcggtcgct ggcggttttc acgcagccgc gctcgtccac gctgccggcc
    14701 gaggaccgca cgaacgcggc cagctgctga tattgctcac ggcttagccg cacttcgcgg
    14761 aaccactggc cctctgcggc gtcggccaga tactcgacgt gcagcaccga ttcgctgggc
    14821 cagaacagcg cgccgacggc ggtgctcagg cgaaactccg accaggtcgg cgtgttgacg
    14881 tagaattcgc gattgcccca gccgacggcg acgtatttgg cagccctcac gtcgcgccga
    14941 aaatgctgcg gcggaaagag ctgccgccaa tcgacgccgg tttcgctcgt caccggcaat
    15001 acaacgtcgg tgtgcacctc gttgctgcgg acgaagatga cgaccgcgtc cgcggtcggc
    15061 gtttgatagc cgcgattgcg cggtgcaaag ccgagcgcca ggaacagggc gtagaaaaca
    15121 aagg
//
LOCUS       DASRJB010000772         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3600611, whole genome shotgun sequence.
ACCESSION   DASRJB010000772 DASRJB010000000
VERSION     DASRJB010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600611"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFB80_20420"
     CDS             <1..375
                     /locus_tag="VFB80_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36211.1"
                     /translation="KVMVAVELAGLMARVKACDGMLKDPKRLEISTLQSISDMKVKAE
                     IEFVGIKEKLDQINAFIGEGNDREAAASRLATAETNKARAQSRLASADNSALSYGELV
                     LIYAPLQIEGNQIIISPIQWTN"
     gene            complement(388..1644)
                     /locus_tag="VFB80_20425"
     CDS             complement(388..1644)
                     /locus_tag="VFB80_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36212.1"
                     /translation="MNRLAVSGAAVLSVVVSTAALACLWDSDTLQMERFRFPGALEVI
                     TGKFVRHSPAYYEWRIADRQKKLVAAPDNPALVDDLAVAYAKLGRTDEGIPLLEQSLA
                     ARPDRYETLSNLGTLHFFAGRLEPSKDYIRRALAINPGAHFGREKYQLLLTEYVQQSQ
                     RAGLGVLSSEYRTPSEQGTIVIGFADFVLRRSDVGRGQSDELLSDARKAEIRAAARGV
                     LGMLHFADHRSPILLEALGDLLLTGRLSEDAAQHAARAYLRAAQFADDPDAAARFRVM
                     AKESLSVQESVYLSPLEESLNAEVAAADEWFAAIAANEQRWIEEGKDVDREFAAAYYQ
                     TLEQVMAGALAESRPAPSNSLYESYRSQADSRQLASLIFAYIAAPLIAFAATFAGVYW
                     LRVRRSRTPPARPKSALGMTQRPPKP"
     gene            complement(1722..2504)
                     /locus_tag="VFB80_20430"
     CDS             complement(1722..2504)
                     /locus_tag="VFB80_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HZN36213.1"
                     /translation="MTTALAPTRAKAARREFLSARWHLLAMLNWEVDPRLLAPLVPRG
                     TELDFHAGKTFVSVVGFLFRETRLLGVSIPWHRHFEEVNLRFYLRRVVEGEVRRGVAF
                     VKEIVPRWAIAQTARVCYNEPYVALPMRHEVSGRAAEVLGVQNSDDVGKVGYGWRLRG
                     RWNSLEVGYAGPRAPLVHGSREEFIAEHYWGYCAQRGGGTVEYQVEHPPWHIWPATAA
                     KLDCDAAALYGPQLAEVLSQPPTTAFLADGSAVAVMKPTRIC"
     gene            2503..>2740
                     /locus_tag="VFB80_20435"
     CDS             2503..>2740
                     /locus_tag="VFB80_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36214.1"
                     /translation="MAMLPLLIPVAFGENNKNRRTEEPKNQRTEEPKNRIDCLFGSLV
                     LWILEFVFVYSPHLRQWSNARNVLLRPWQAKPEAA"
BASE COUNT          482 a          919 c          893 g          446 t
ORIGIN      
        1 aaggtgatgg tcgccgtgga gctggccggc ctcatggccc gcgtcaaggc gtgcgacggg
       61 atgctgaagg atcccaagcg gctggagatc agcacgctgc agtcgattag cgacatgaaa
      121 gtgaaagctg aaatcgagtt cgtcggcatt aaggagaagc tcgaccagat caacgccttc
      181 atcggcgaag ggaatgaccg cgaggcggcc gcgtcgcgtc tggccacggc ggagaccaac
      241 aaagctcggg ctcaatcccg cctcgcatcc gccgacaatt cggcgctgtc gtacggcgag
      301 ctcgtcttga tctacgcccc cctgcaaatt gaggggaacc aaatcatcat cagcccaatc
      361 cagtggacga attgacgcgg ctcgctgtca tggcttcggc gggcgctgcg tcataccgag
      421 tgccgacttg ggtctggccg gcggagtgcg gcttcgcctg acccgcagcc aatacacgcc
      481 tgcgaaggtc gccgcgaagg cgatcagcgg ggcggcgatg taggcgaaga tcaggctcgc
      541 gagctggcgg ctgtcggcct ggctgcggta cgactcgtac agcgagttgc tcggcgcggg
      601 ccgggattcg gccagcgcgc ccgccatcac ctgctccagc gtctggtagt aagcagccgc
      661 aaactcgcga tcgacgtcct tgccttcctc gatccaccgc tgctcattgg cggcgatggc
      721 cgcgaaccat tcgtcggccg cggccacctc ggcgttcaag gactcctcca gcggcgagag
      781 gtagacgctc tcctgcaccg acagcgactc tttggccatg acgcggaacc gcgcggcggc
      841 gtcgggatcg tcggcgaact gggccgcccg caggtaggcc cgcgcggcgt gctgggccgc
      901 gtcttcgctg agcctgcctg tcagcagcag gtcgcccaag gcctccagca ggatcggcga
      961 gcggtggtcg gcgaagtgca gcatgccgag cacgcccctg gcggcggcgc ggatttccgc
     1021 cttgcgggcg tcgctcagca gctcgtcaga ctggcccctt cccacatctg agcggcggag
     1081 gacgaagtcg gcaaagccga tgacgatcgt gccctgctcg ctgggcgtgc ggtactcgct
     1141 cgacagcact cccagcccgg cccgctggct ctgctgcacg tattcggtga gcagcaactg
     1201 atatttctcg cggccgaaat gagcgccggg gttgatcgcc agcgcgcggc ggatgtagtc
     1261 cttgctcggc tcgaggcggc cggcgaagaa gtgcagcgtg ccgaggttgg agagcgtctc
     1321 gtagcggtcc ggccgcgcgg ccagcgactg ctccaggagc ggaatgccct cgtccgtccg
     1381 gccgagcttc gcataggcca cggccagatc gtccaccagc gccggattgt ccggcgcggc
     1441 cacgagcttc ttctggcggt cggcgatccg ccactcgtaa taggccgggc tgtgccggac
     1501 gaacttgccg gtgatgacct ccagcgcgcc gggaaagcgg aaccgctcca tttgcagcgt
     1561 gtccgaatcc cacaggcagg ccagggccgc tgtggacact accaccgaca atactgccgc
     1621 gccactgaca gccaggcggt tcatgcgctc ctcgttcgcg gccagcgccg attctgctcg
     1681 ccaactccat tgtacggcgt tcacacgccg cgcgcgtcgg cttaacaaat ccgcgtcggc
     1741 ttcatcaccg cgaccgcgga gccgtcggcg aggaaggcgg tggtcggagg ctgcgagagc
     1801 acttcggcca gctgcggccc gtaaagcgcc gcggcgtcgc agtcgagctt tgccgcagtc
     1861 gccggccaga tgtgccaggg cggatgctcg acctggtatt caaccgtgcc gccgccgcgc
     1921 tgagcgcaat agccccagta gtgttcggcg atgaactcct cgcgtgatcc gtgcacgagc
     1981 ggcgcgcggg gacctgcgta gccgacttcc agcgaattcc accggccccg cagccgccag
     2041 ccgtagccga ccttccccac gtcatccgaa ttctgcacgc cgagcacttc ggccgcccgc
     2101 ccgctgacct cgtgccgcat cggcagggcc acgtagggct cgttgtagca gacccgcgcc
     2161 gtctgcgcga tggcccaccg cgggacgatc tccttgacga acgccacgcc gcggcggact
     2221 tcgccctcca ccacgcggcg cagatagaac cgcaggttca cttcctcgaa gtgccggtgc
     2281 cagggaattg aaacgcccaa gagccgcgtc tcgcggaaca ggaagccgac gacgctgaca
     2341 aaggtcttgc cggcgtgaaa atcgagctcc gtgccgcggg gcacgagcgg cgccagcagc
     2401 cgcggatcga cctcccagtt cagcatcgcc agcaggtgcc agcgggccga gagaaattcc
     2461 cggcgcgcgg ctttcgcgcg ggtcggcgcc agcgcggtcg tcatggcgat gctcccgctg
     2521 ctcataccgg tcgcctttgg cgagaataac aagaaccgaa gaaccgaaga accaaagaac
     2581 caaagaaccg aagaaccgaa gaaccgaatt gattgcctat ttggttcttt ggttctttgg
     2641 atcttggaat ttgttttcgt ctactcccca catctccggc agtggagcaa cgcacgtaat
     2701 gtactcctcc gcccttggca ggcgaaaccg gaggctgcgg
//
LOCUS       DASRJB010000773         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2482221, whole genome shotgun sequence.
ACCESSION   DASRJB010000773 DASRJB010000000
VERSION     DASRJB010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482221"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2175)
                     /locus_tag="VFB80_20440"
     CDS             complement(<1..>2175)
                     /locus_tag="VFB80_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HZN36215.1"
                     /translation="QTILQEPEFRDDPSVGVVIQCYLRDSGADLAALRDWARRRGTPV
                     WVRLVKGAYWDFETIRAKASGWPIPVYQKKWESDANFETQTRFVMQNADVLRPALGSH
                     NLRSLAHGIAVAEHLGLPKSSFEIQMLYGMADAQKQALVDLGHRLRIYMPYGDLIPGM
                     AYLVRRLLENTSNDSFLRAGFFDEVPPDELLKSPAAIGKVSEYEGMRVCGNSDTESTR
                     APSYPRTLTPGIQPKESDMTAARNFAPTFINQPPVDFSLEENCQAMREALATVKHRLG
                     RDCPLVIGGQSIDTPDKAESRDPSHRQRVVGRFSLADKEHVQQAVAAAKSALPQWWQL
                     GVEERAEFLRAAAEQMRQRLFELAAWIVYETGKPWREATADVDEAIDFCEFYAAEAIK
                     LQSPAGVDVPGEENRFEYLPRGVAAVIAPWNFPLAILTGMTTAALVTGNTVVMKPAEQ
                     SSVIASMLMEIFESAQLPPGVLNYLPGKGEIAGAALVEHPDVAIIAFTGSRSVGLAIN
                     STAAEVSRRGTGQVKRVIAEMGGKNAIIVAADADLDEAVTGVAKSAFVYQGQKCSACS
                     RAIVVADVYDAFLARLVETTRSMKVGPAEEPSTLIGPVIDDEALARIKEYIEVGKKEG
                     RLLLAGDVGPLAQQGCFVAPHIFVDVPPTARIAQEEIFGPVLAVIKASDLTEALRIAN
                     GTEYALTGGVYSRSPENIRRVKREFRVGNLYVNRKITGALVGR"
BASE COUNT          363 a          709 c          706 g          397 t
ORIGIN      
        1 cggccgacga gcgcgccggt gatcttgcga ttgacgtaca gattgccaac gcggaactcg
       61 cgcttgacgc ggcgaatgtt ctccgggctg cgcgaataga cgccgccggt cagggcgtat
      121 tcggtgccgt tggcgatccg cagggcctcg gtcagatcgg acgctttgat cacggcgagc
      181 accggcccga agatttcttc ctgcgcgatg cgggcggtcg gcggcacatc gacaaaaatg
      241 tgcggggcca caaagcaacc ctgctgggcc agcgggccga cgtcgccggc caggagcagc
      301 cgaccttctt tcttgccgac ttcgatgtac tccttgatcc gcgccagcgc ctcgtcgtcg
      361 atgactgggc caatgagggt cgagggctct tcggccgggc cgaccttcat gcttcgcgtc
      421 gtctccacga ggcgcgctaa aaaagcgtcg tagacgtccg ccacgacgat cgcccggctg
      481 cacgcggagc acttctgtcc ctggtagacg aacgcgctct tggccacgcc ggtcactgcc
      541 tcgtccaggt ccgcgtcggc cgccacgatg atcgcgttct tgccacccat ctcggcaatc
      601 actcgcttca cctggccggt gccgcggcgc gagacctcgg ccgccgtgga gttgatcgcc
      661 aggccgacgc tccgcgagcc ggtgaaggcg atgatcgcca cgtccggatg ttcgaccagt
      721 gccgcaccgg caatttcccc ttttcccggc aggtagttga gcacacccgg cggcaactgg
      781 gcgctctcga agatctccat cagcatggag gcgatgaccg acgactgctc cgccggcttc
      841 atgacaaccg tgttgcccgt gacgagtgcc gccgtggtca tgcccgtgag aatcgccagc
      901 gggaagttcc acggggcgat gacggccgcg acgccgcggg ggagatactc aaagcggttc
      961 tcctcgccgg ggacatcgac gcccgcgggt gactgaagct tgatcgcctc ggcagcatag
     1021 aactcgcaga agtcgatggc ttcatccacg tcggcggttg cttcgcgcca cggcttgccc
     1081 gtttcgtaga cgatccaggc cgccagctcg aacagccgct ggcgcatctg ctcagcggcg
     1141 gcgcgcagga attccgcccg ctcctcgacg cccagttgcc accactgcgg cagggcgctc
     1201 tttgccgcgg ccaccgcttg ctgcacgtgc tccttgtcgg ccagcgagaa tcggccgacg
     1261 acgcgctggc ggtgcgacgg atcgcgggac tcggccttgt cgggcgtgtc gatcgactgt
     1321 ccgccgatca ctagcgggca gtcgcggccc aggcggtgtt tgaccgtggc cagcgcctcg
     1381 cgcatcgcct ggcagttttc ttcgagggaa aaatcgacgg gaggctggtt gataaaggtg
     1441 ggagcgaagt ttctggccgc tgtcatatcg ctctcctttg gttgaatgcc gggtgtgagg
     1501 gtacgagggt atgagggcgc gcgagtggac tcagtgtcgg aattcccgca cacccgcata
     1561 ccttcatact ccgacacctt tccgattgcc gccggagact tcaatagttc gtccggcggc
     1621 acttcgtcga agaatccggc tcgcaaaaac gaatcgttcg acgtgttttc cagcagccgc
     1681 cgcaccaggt acgccatgcc ggggatcagg tcgccatacg gcatataaat ccgcaaccga
     1741 tgacccaggt ccaccagcgc ctgcttctga gcgtccgcca tgccgtagag catctggatt
     1801 tcaaagctgc tcttgggcag gcccaggtgc tcggcgacgg caatgccgtg ggccagcgag
     1861 cggaggttgt gcgagccgag ggcgggccgc agcacgtcgg cattctgcat gacgaagcga
     1921 gtctgcgtct cgaagttggc gtccgactcc cacttctttt ggtaaacggg aatgggccag
     1981 ccactggcct tggcacggat cgtttcgaaa tcccagtagg cgcctttcac cagccgcacc
     2041 cagaccggcg tgccgcggcg gcgggcccag tcgcgaaggg cggcaaggtc tgcccccgag
     2101 tcgcggaggt agcactggat gacgacgccg acgctcgggt cgtcgcgaaa ttccggctcc
     2161 tggaggatcg tctgg
//
LOCUS       DASRJB010000774         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4174956, whole genome shotgun sequence.
ACCESSION   DASRJB010000774 DASRJB010000000
VERSION     DASRJB010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174956"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB80_20445"
     CDS             complement(<1..574)
                     /locus_tag="VFB80_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36216.1"
                     /translation="MFLSRRQRGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRSS
                     CQNNLKQIGIATHNFHDTFNFLPPLRLAGGDGWATYWVLIMPYMEQGNLQQQWNLQLK
                     YSVQSAAARTAQIKSYYCPSRRSGGVSVAEQFWVTDQTAPPNPASAGSTEVRFGPTNN
                     VSGATGDYAACVGDMRGLPNNPSAQNWFNTN"
     gene            916..1527
                     /locus_tag="VFB80_20450"
     CDS             916..1527
                     /locus_tag="VFB80_20450"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN36217.1"
                     /translation="MAQGTKNLDPRWGFSRRDFLRGSGAVAAATAIAQQSTAALAQEK
                     GAKVTSGATTITLDVNGQSKQVKVEPRTTLLEVLRYQLDLTGAKPICTDGSSGGATVL
                     VDDKPMQANTMLALQAVGKKIRTVESLAGSAVPNAFVTCDAQQCGFCTPGFVVGVKAF
                     LDKSPKATEEEIRKGLNGNLCRCGTYANVIHAALMVVKGGANG"
     gene            1520..3769
                     /locus_tag="VFB80_20455"
     CDS             1520..3769
                     /locus_tag="VFB80_20455"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN36218.1"
                     /translation="MAEYSWPTKDKTTAIGKRVKRLDGPEKSTGKAKYTYDVNLPNML
                     VAKALGCPHAHCKITSIDVAPALKVPGVVAAEPMEKGDRPVQSGDVIEFQGDLLAIVV
                     AENEGAAAEGVKAIDVKYEPLPSFTKDDDLAAAQAAGRVSKGGNKTLLEAMPKENEDA
                     AAFENAEIERLLKAAAHTVEGTYGIDAITHCCLEPHGSTMQWDGNKLIAYVSTQAVSN
                     NEDGFAGALKIGANDVEIKCDYIGGGFGSKFNPDYWAVHAAKLAKKTGRPVKFMLSRD
                     QELKIAGNRPSAYIKVRLGADKDGLVTVWDSEHWGTTGLTPGGVNHAQVPYVFQPKNY
                     RRVQTNIKTNNSQSRAWRAPNHPQACAVTQTALDDLAMKMGADSLEIFIKNLGTDDKP
                     LNTQTGGNPKPSLYKEEFEIGAKLIDWKAKWHAHGKGQARGSVVDGLGCAMHTWGGGA
                     GNSNCTVRVYPNGDVETMCGSQDLGTGTRTVIALVLAETFGLPYEGVRVNIGTSKYPV
                     SNASGGSTTVGAVAESHRRAGQDALAEIAALVGKKLEVDPATLEAVGGKVQVKGNPAK
                     SVSWKEACSLLGMKPLEKTGSFNRNQDARTSKLSGLQVGGIQMAHVEVDKETGVVRMK
                     KFVAVQDMGLIISPQQAESQINGALIMGIAYALFEHRLNDPASGAFVNAELKDYKLAR
                     LGDIGELVVHLHEPESERARGVIGLGEPPVIACGAAISNAVANALGVRVPTLPLTPER
                     VLAALKKKA"
BASE COUNT          817 a         1295 c         1318 g          600 t
ORIGIN      
        1 tgttcgtatt gaaccaattc tgagcactag gattgttggg caggccccgc atgtcgccga
       61 cgcaggccgc gtaatcgccg gtggcgccag acacgttatt cgtcggccca aaccggacct
      121 ccgtgctgcc agcactggcc ggattgggcg gggccgtctg gtccgtcacc cagaattgtt
      181 cggccacgct cacgccgccc gaccgccgcg acgggcagta gtacgacttg atctgggccg
      241 tccgcgctgc tgccgactgc acggaatact tgagctgcag gttccactgc tgctgcaaat
      301 tgccctgttc catatagggc atgatcagca cccagtaggt cgcccagccg tcgccgcccg
      361 ccagccgcag cggtggcagg aagttgaacg tgtcgtgaaa gttgtgcgtc gctatgccaa
      421 tctgcttgag gttgttctgg caggaggagc ggcgggccgc ttcgcgggcg gcttgaaccg
      481 cgggaagcag cagggcgacg aggacgccga tgatggcgat gacgacgagc agctcaacga
      541 gcgtgaagcc gcgctggcgg cgggagagga acatgcggcg aaaactccca gaaaaatcca
      601 gaattggctt tgcggccgcc ggcagaactg ccgcggcatt cacggattct acctaaaggt
      661 ttcaaccgtg taattcgcaa ttcgggcaaa ttccattgca aatcgggcaa tgcatcgtgc
      721 cactttgtcg cgatgcaaaa ttccgcctca aatcaattcc ccttgattgc aagtggcccc
      781 tgcagccgca ataatctgcg gcgcgtgctg gctcctcccg cggcgttagc ggcgaatcga
      841 ttcaccttag tttcgcggga gattgacggc ggcaacgatt ggcagcatcc acgccacgtt
      901 ttcaggaatc acctcatggc acaaggcacc aaaaacctgg atccccgctg gggattcagt
      961 cgccgcgatt tcctgcgggg atcgggcgcg gtggccgcgg caacggcgat cgcccagcaa
     1021 tcgaccgccg ccctggccca ggaaaaaggc gcaaaagtca ccagcggcgc gaccacgatc
     1081 acgctcgacg tcaacgggca gtcgaagcag gtcaaggtgg agccgcggac cacgctgctc
     1141 gaagtgcttc gctaccagct cgacctgacg ggggccaagc cgatctgcac cgacggctct
     1201 tccggcgggg cgacggtcct ggtcgacgac aagccgatgc aggccaatac gatgctcgcc
     1261 ctgcaggcgg tcggcaagaa gatccgcacg gtcgagtcgc tggcgggtag tgccgtaccc
     1321 aatgccttcg tgacctgcga cgcccagcag tgcggcttct gcacgccggg cttcgtcgtc
     1381 ggggtgaagg cgtttctcga caagagtccg aaggcgacgg aagaggaaat ccgcaaggga
     1441 ctcaacggca acctctgccg ctgcgggacc tatgccaacg tcattcacgc ggccttgatg
     1501 gtcgtgaagg gaggggccaa tggctgagta ttcctggccg accaaggaca agaccaccgc
     1561 catcggcaag cgcgttaagc gcctcgatgg tccggaaaaa tcgaccggca aggcgaagta
     1621 cacctacgac gtgaacctgc cgaacatgct cgtcgccaag gccctgggct gcccgcacgc
     1681 gcactgcaag atcacgtcga tcgacgtggc cccggcgctg aaggtgcccg gcgtcgtcgc
     1741 cgccgagccg atggaaaaag gcgatcggcc ggtccagagc ggcgacgtca tcgagttcca
     1801 gggggacctg ctggccatcg tcgtcgccga gaatgagggc gcggcggccg aaggcgtcaa
     1861 ggccatcgac gtcaagtacg aacccctgcc gtcgttcacc aaggacgacg acctggcggc
     1921 cgcccaggcc gcgggccgcg tcagcaaggg cggcaacaag acgctcctgg aggcgatgcc
     1981 caaggaaaat gaggacgccg ccgccttcga gaacgcggag atcgagcggc tgctcaaagc
     2041 cgcagcccac acggtcgagg gcacgtacgg catcgacgcc atcactcact gctgcctgga
     2101 gccccacggg tcgaccatgc agtgggacgg caacaagctg atcgcttacg tctcgacgca
     2161 ggcggtctca aacaacgagg acggcttcgc cggcgcgctg aagattggcg ccaacgacgt
     2221 cgagatcaag tgcgactaca tcggcggcgg cttcggcagc aagttcaacc cggactactg
     2281 ggcggttcac gcggccaagc tggccaagaa gaccggccgg ccggtgaagt tcatgctttc
     2341 ccgcgaccag gagctgaaga tcgccggcaa ccgccccagc gcgtacatca aagtccgcct
     2401 gggcgcggat aaggacggcc tcgtcacggt ctgggactcg gagcactggg gcacgacggg
     2461 cctcaccccc ggcggcgtca accacgccca ggtcccgtac gtcttccagc cgaagaacta
     2521 tcggcgggtg cagacgaaca tcaagaccaa caacagccag tcgcgagcct ggcgggcccc
     2581 gaaccatcca caggcctgcg ccgtcacgca gacggcgctc gacgacctgg cgatgaagat
     2641 gggggccgac agcctcgaga tcttcatcaa gaacctgggg accgacgaca agccgctcaa
     2701 tacccagacg ggcggcaacc ccaagccttc gctctacaag gaggagttcg agatcggcgc
     2761 gaagctgatc gattggaagg ccaagtggca cgcgcacggc aagggccagg cgcggggttc
     2821 ggtcgtggac ggcttgggct gcgcgatgca cacttggggc ggcggcgcgg gcaactcgaa
     2881 ctgcaccgtc cgcgtctatc ccaacggcga cgtggagacg atgtgcggct cgcaggacct
     2941 gggcactggc acccgcacgg tgatcgccct ggtgctggcc gagacctttg gcctgcccta
     3001 cgagggggtc cgggtcaaca tcggcacgtc gaagtacccg gtgagcaacg ccagcggcgg
     3061 cagcaccacc gtcggcgccg tcgccgagtc gcaccgccgc gccgggcagg acgccttggc
     3121 cgagatcgcc gccctggtcg gcaaaaagct ggaggtcgac ccggccacgc tggaagcggt
     3181 cggcggcaag gtgcaagtga agggcaaccc tgccaagagc gtgagctgga aggaagcctg
     3241 ctcgctcctg ggcatgaagc cgctggagaa gaccggctcc ttcaaccgca atcaggacgc
     3301 tcgcaccagc aagctctcgg gcctgcaggt cggcggcatc cagatggccc acgtcgaggt
     3361 cgataaggag accggcgtgg tacggatgaa gaagttcgtc gccgtgcagg acatgggcct
     3421 gatcatcagc ccgcagcagg ccgagagcca gatcaacggc gcgctgatca tgggcatcgc
     3481 ctacgcgctg ttcgagcacc gcttgaacga tcccgcgagc ggggcgtttg tcaacgccga
     3541 gctgaaggat tacaagcttg cgcggctggg cgacatcggc gagctggtcg tgcacctgca
     3601 cgagccggag agcgagcggg cccgcggcgt gattggcctg ggcgagccgc cggtgattgc
     3661 ctgcggcgca gcaatcagca acgcggtcgc gaacgccctc ggcgtccgcg tgcccacgct
     3721 cccgctcacg cccgaacgcg ttctggccgc cttgaaaaag aaagcgtaac aaagccgcga
     3781 ccattggtcg cgctctgata gggaacgccc tccgcggcat tccctcgcct accaaacaca
     3841 cgacactcct ccgggagaaa tcacatgaaa gacttcgaat acgcatcgcc cacgacggag
     3901 gccgaggtgc tgatgcacct ggcggcctgg cccggcgaga cggaattgat cgccggcggg
     3961 accgacctcg tcggtctgct gcaaaagatg gttatcacgc ccaagcgcgt ggtgaacctg
     4021 ctcgacgtgc
//
LOCUS       DASRJB010000775         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3601899, whole genome shotgun sequence.
ACCESSION   DASRJB010000775 DASRJB010000000
VERSION     DASRJB010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601899"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFB80_20460"
     CDS             <1..516
                     /locus_tag="VFB80_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN36219.1"
                     /translation="TQPVIILTARGEESDRVEGLRAGADDYVVKPFSVKELLARVEAV
                     LRRSPGRPSDMPEVKIPGGVADFSRRELRFADGERCELSEREVELLRYLANHSERAVA
                     RDELLANVWRISPDGLPTRTIDMHVARLREKLRDDSADPQVLLTVRGKGYMFKTPKNQ
                     RTEEPKNRRSE"
     gene            569..2545
                     /locus_tag="VFB80_20465"
     CDS             569..2545
                     /locus_tag="VFB80_20465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN36220.1"
                     /translation="MKRPWQIWLLYAVGLAGVAGAFGWLTVKALELDRAESLARRQAE
                     LEEDVSSALWRMDAELTPILADEATRPDFVYHPVYPTEPPVKGGKPPAQELSPLLVRP
                     PNFVLLHFEVHPDGCVISPQNPVGDDNAWALRNGASLERVNAAARLLEELKPALSIDA
                     LLAQLPQHSVPASGPWQPPGQPGGNSAAIVNNYDFAQQARQQQLEQAVQPQPPQYLVN
                     PPAQQPAANPGQEDSQPPAQSQSGGQQAGSQLAYNNSGYGNNVESGRLQRSQSRADND
                     LPNRQGVFQAAAQRAAEQRGNLRGFQPAVRVVEGASRVLWLGDHLVMARRVQIGEQTV
                     VQGCWLDWQQLKEMLRWKIADVLPEVELTPLKGTERIKLSRVLATIPVQLSVPAPTLP
                     PVAWTPIRVSLVVAWLCLVATALAAALMLHSVVALSERRGAFVSAVTHELRTPLTTFR
                     MYAEMLSAGMVPEGQQRQHYLETLRIEADRLAHLVENVLQYARLERGRPGKRRESVSI
                     HGLLDRSQARLADRAAQAEMKLVVELAGEDAEVSLATDSAAVEQILFNLVDNACKYAV
                     NAADKRIHLAIAVQPRQVQFTVRDHGPGISASGRRRLFQPFSKSVHEAANTAPGVGLG
                     LALSRRLAGELGGRLQLNSPVGGGCAFVLTLPRT"
     gene            complement(2600..3151)
                     /locus_tag="VFB80_20470"
     CDS             complement(2600..3151)
                     /locus_tag="VFB80_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZN36221.1"
                     /translation="MKSLQGHFLIASPKLADGNFYKSVVLMIKHDEEGAFGLILNRPT
                     ENSVGDVWKLVGEAQIDCPQPIFLGGPVTGPLVALHRLKSAAEMEVLPGLYFAAHKDQ
                     LQKIATQTAKPFRFFSGYAGWAGGQLEGELKAGGWLTAKAKKDLIFHKGDDLWEQVVR
                     TIGEGILGAAIKAKHVPENPSLN"
BASE COUNT          548 a         1088 c         1116 g          570 t
ORIGIN      
        1 acgcagccgg tgatcatcct cacggcccgc ggcgaagaga gcgaccgcgt ggaagggctg
       61 cgggccgggg ccgacgacta tgtcgtcaag ccattcagcg tgaaggagct cttggcgcgg
      121 gtcgaggccg tgctgcggcg ctcgcccggc cggccgagcg acatgcccga ggtgaaaatc
      181 cctggcggcg tggccgactt ctcccgccgc gagctgcggt tcgccgatgg cgagcggtgc
      241 gagctgtccg agcgtgaggt cgagctgctg cggtacctgg cgaaccattc cgagcgggcc
      301 gtcgcccgcg acgaactgct tgctaacgtg tggcggatca gccccgacgg cctgcccacc
      361 cgcacgatcg acatgcacgt cgcccgcctc cgtgagaagc tccgcgacga ctcggccgat
      421 ccgcaagtgc tgctgaccgt gcgggggaag gggtatatgt tcaaaacgcc aaaaaatcaa
      481 agaaccgaag aaccaaagaa ccgaagaagc gaatgagagc acaacgtacg agttttggtt
      541 cttcggttct ttggttcttg gagtttccgt gaagcgtccc tggcaaatct ggctcctcta
      601 cgccgtcggc ctggccgggg tggcgggggc gttcggctgg ctgacggtca aggccctgga
      661 gctggaccgc gccgagtcgc tggcccgccg gcaggcggag ctggaagagg atgtcagctc
      721 ggccctctgg cggatggatg cggagctgac cccgattctg gcggatgagg cgacgcggcc
      781 cgactttgtc tatcaccccg tctatccgac ggagccgccg gtcaaaggcg gcaagccccc
      841 ggcgcaggag ctgtcgccac tactggttcg gccgccgaat ttcgtgctgt tgcacttcga
      901 ggttcatccc gacgggtgcg tcatttcccc gcagaatccg gtgggcgacg acaacgcctg
      961 ggccctccgg aacggggctt cgctcgagcg ggtcaatgcg gctgcccggt tgctggagga
     1021 gttgaagccc gcgctgtcga tcgacgcgct cttggcccaa ttgccgcagc attccgtgcc
     1081 agccagcggg ccttggcaac cgccagggca accgggcggg aatagcgcgg ccatcgtaaa
     1141 caactacgac tttgcccagc aggcccggca gcagcagctc gaacaagccg tacagccgca
     1201 gccgccgcaa tatctggtca atccgccggc tcagcagccc gcggcgaacc ccgggcaaga
     1261 agattcacaa ccaccggcgc agtcgcaatc cggcgggcag caagcgggtt cgcagttggc
     1321 gtataacaat tcgggctatg gcaacaacgt cgagagcggc cgcttgcaac ggtcccaatc
     1381 gcgagccgac aacgacctgc ccaaccgcca gggggtattt caagccgcgg cccagcgggc
     1441 tgcggaacag cgcggcaatc ttcgaggctt ccagccggcc gtccgcgtcg tcgagggcgc
     1501 tagccgcgtc ctgtggctgg gcgaccacct ggtcatggcc cggcgcgtgc aaatcggcga
     1561 gcaaacggtg gtgcagggct gctggctgga ctggcagcag ctgaaggaga tgctgcggtg
     1621 gaaaatcgcc gatgtgctgc ccgaagtgga gctgacgccg ctcaagggga cggagcggat
     1681 caagctcagc cgcgtgctgg cgacgattcc cgtgcagctc tcggtgcccg cgccgacctt
     1741 gccacccgtg gcttggacgc cgatccgcgt gtcgctggtg gtggcctggc tgtgcctggt
     1801 ggcgacggcg ctggccgccg cgctgatgct gcacagcgtc gttgccctca gcgagcggcg
     1861 cggcgctttt gtttcggccg tgacgcacga gctccgcacg ccgctgacga cgtttcgcat
     1921 gtatgccgag atgctctcgg ccggcatggt gcccgagggc cagcagcggc agcattacct
     1981 ggagacgctg cgaatcgaag ccgaccgcct ggcgcacctg gtcgagaacg tgctgcaata
     2041 cgcccgcctg gagcgcggcc ggcccggtaa gcgccgcgag agcgtctcaa ttcacggcct
     2101 gctcgaccgc agccaggccc gcctcgccga tcgcgccgcg caggcggaaa tgaagctcgt
     2161 cgtcgagctg gctggcgaag acgcagaggt ctcgctcgcc accgattccg ccgccgtcga
     2221 gcaaatcctg ttcaacctgg tcgataatgc ctgcaagtac gcggtcaatg cggccgacaa
     2281 gcggattcac ctggcgattg ctgtgcagcc gcggcaggtg cagttcaccg ttcgcgatca
     2341 cgggcctggc atttctgcgt ccggccgccg gcggctgttt cagccgttct cgaaatccgt
     2401 acacgaggcg gccaacaccg ctcccggcgt cggcctcggc ctggccctca gccggcgact
     2461 ggcgggcgaa ctcggcggcc gcttgcagct caattcaccc gtcggcggcg gctgtgcgtt
     2521 cgtcctgacg ctcccgcgca cgtagagcaa gcgtcctcgc ttgctcggtc tgaacaagcg
     2581 aggacgcttg ttctacgtct cagttcagcg atggattctc gggcacgtgc ttggccttga
     2641 tggcggcgcc gagaatgccc tcgccgatcg tgcggacgac ttgctcccac aggtcgtccc
     2701 ccttgtggaa gatgagatcc ttcttggcct tggcagtcag ccaaccgccg gccttcaact
     2761 cgccttcgag ctggccgccc gcccagccgg cgtagccgct gaaaaagcgg aacggcttgg
     2821 cggtctgcgt ggcgatcttt tgcagctggt ccttgtgggc ggcgaaatac aggcccggca
     2881 ggacctccat ttcggcggcc gacttgagcc ggtgcaaagc gaccagcggc ccggtgaccg
     2941 gcccgcccag gaagatcggc tgcgggcagt cgatctgggc ctcgccgacc agcttccaga
     3001 cgtcgcccac gctgttttcg gtcggccggt tcaggatcag tccgaatgcg ccttcctcat
     3061 cgtgcttgat catcagcacg accgatttgt agaagttccc atcggccagc ttgggcgagg
     3121 cgatgagaaa atggccttgg agcgatttca tggcgcggcc tggggctacc gcaattctag
     3181 tccgccgccc ctcgtagagt aagcgtcctc gcttgctcgg cgctgtcggc acgccggttc
     3241 ttgcgggcga tttggacgac gatcagcagc ggctgtgaga tggcttgggc cgggcctcgg
     3301 caacttgctc ggaacacctc gt
//
LOCUS       DASRJB010000776         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2756828, whole genome shotgun sequence.
ACCESSION   DASRJB010000776 DASRJB010000000
VERSION     DASRJB010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756828"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFB80_20475"
     CDS             <1..291
                     /locus_tag="VFB80_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36222.1"
                     /translation="TCRQAAGIHYVALAPQARGPQYAWVGCLLKNEQGEWAAHTPLLP
                     PAAAGSLAVLRKDSAGAIAAQPIGRAESGKAALDRSAADALTEGRPIYLVGE"
     gene            297..995
                     /locus_tag="VFB80_20480"
     CDS             297..995
                     /locus_tag="VFB80_20480"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HZN36223.1"
                     /translation="MSYLIRLRGQQQGPFTADQLQKLASRGRFSRSYEVSTDDGVSWQ
                     RAEAFPELFPKLPPRRGETAPATADKAATAAAEQPGEEWYYTHAGAELGPTSVGELKR
                     LIGLGQVVAEDHVWTEGMDYWQRVRDVPELARQLTANTAAALPPTPQTSPMAVASFIL
                     GLLGFTLIGSILAIVFGHIALSQIRQSQGKLGGKGLAIVGLVLGYLVVVTSVIAAIVV
                     IAILALAPSGSRIT"
     gene            1032..1901
                     /locus_tag="VFB80_20485"
     CDS             1032..1901
                     /locus_tag="VFB80_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN36224.1"
                     /translation="MDRNLDVPLAWERDDIEQRWGFKGGRFTRVNLLFSALVALCLTV
                     GFFILMIVCRRYQVPVLGRIGTSFLERGPIPYPTAFFAFWSLTILWIKWRKLKYQERA
                     LSYSVVPADHDFVLSPATVDVVMDNIHATVDDPRKFVIFNRIMVALSNLRNLGVVGDV
                     DDILRSQAENDESGLETSYAMLAGFVWSIPVLGFLGTVQGLSQAIGNFGSVLQAAAEI
                     SAIKVALQSVTGGLATAFETTFQALVAALIIQLSLVYLKKSEEEFLDACGDYCLRNVV
                     NRLRMMPFQTEQV"
     gene            1917..>2098
                     /locus_tag="VFB80_20490"
     CDS             1917..>2098
                     /locus_tag="VFB80_20490"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HZN36225.1"
                     /translation="MSQWYCKLDGAAAGPFAADELAYLACRGRLRADTEVRLAEERSW
                     SPAGERLSALFPAAQPS"
BASE COUNT          329 a          699 c          699 g          371 t
ORIGIN      
        1 acgtgccggc aggccgccgg aattcactac gttgcgcttg ccccccaggc ccgcggcccg
       61 cagtatgcct gggtcggctg cctgctgaag aatgaacagg gcgagtgggc cgcccacacg
      121 ccgctcttgc cgccggccgc cgccggctcg ctggccgtgc ttaggaaaga cagcgccggc
      181 gcgatcgcgg cccagccgat cggccgcgcc gagtcgggca aagccgccct cgaccgttcc
      241 gccgccgacg ccctcaccga aggccggccg atttacctgg tgggtgaata gagcgcatgt
      301 cctatctcat tcgccttcgc ggacagcagc aaggcccgtt caccgccgac cagctgcaga
      361 agctggcctc gcgcgggcgg ttcagccgca gctacgaagt ctcgacggat gacggcgtga
      421 gctggcagcg ggcggaggcc tttccggagc tgtttcccaa gctgccgccg cggcgcggcg
      481 agactgcgcc ggcgacggcc gacaaggcgg caactgcggc cgccgagcag cccggcgagg
      541 aatggtacta cacccacgcc ggcgcggaat tgggcccgac cagcgtgggg gagctcaagc
      601 ggctgatcgg gctgggtcag gtggtggccg aggaccacgt ctggaccgag gggatggact
      661 attggcagcg cgtccgcgac gtgccggagt tggcccgcca attgaccgca aacacggccg
      721 ccgcacttcc ccccacgccg caaacgtcgc cgatggcggt ggccagcttc atcctgggcc
      781 tcttgggctt cacgctgatc ggcagcattc tggcgatcgt cttcggacac atcgccctgt
      841 cgcaaatccg ccagtcgcag ggaaagctgg gcgggaaagg gctggcgatt gtggggctgg
      901 tcttgggata cttggtggtc gtcacatcgg tgatcgccgc gatcgtggtg attgcaattc
      961 tggccctggc gccgagcggg tcgcgaatca cctgaatcac tcaacgagaa caacttgcga
     1021 ggagctactg ggtggatcgg aacctggacg tcccgctggc ttgggagcgc gacgacatcg
     1081 agcagcggtg gggcttcaaa ggcggccggt tcacgcgcgt gaacctgctc ttctcggccc
     1141 tcgtcgcgct ctgcctgacc gtggggttct ttattttgat gatcgtctgc cgccgctacc
     1201 aggtgccggt gctggggcgg atcggcacct cattcttgga gcgcgggccg atcccctatc
     1261 ccacagcgtt cttcgcgttc tggtcgctga cgatcctctg gatcaagtgg cggaagctca
     1321 agtaccaaga gcgggccctg agctattcgg tcgtacccgc cgaccacgac ttcgtccttt
     1381 cccccgcgac ggtcgatgtc gtgatggaca acattcacgc cacggtcgat gacccgcgga
     1441 agttcgtgat cttcaaccgg atcatggtgg ccctttcgaa tctgcgaaac ctgggtgtcg
     1501 tcggcgacgt ggacgacatc ctccgctcgc aggccgagaa cgacgaatcc ggcctggaga
     1561 ccagctacgc catgctggcc ggcttcgtct ggtcgattcc cgtgctcggc ttcctgggca
     1621 cggtgcaggg cctgtcccag gcgatcggca atttcggctc ggtgcttcag gccgccgccg
     1681 aaatctccgc gatcaaagtc gccctgcaat cggtgaccgg cggcctggcg acggcgttcg
     1741 agacgacgtt ccaggcgctg gtcgcggccc tgatcatcca gctctcgctc gtgtacctca
     1801 aaaagtccga ggaggagttc ctcgatgcgt gcggcgatta ttgcctgcga aacgtcgtca
     1861 accgcctgcg gatgatgccg ttccagacgg agcaggtcta gccccgggag gccctcatgt
     1921 cgcagtggta ttgcaagctc gacggcgcgg ccgcagggcc ctttgccgcc gacgagctgg
     1981 cctacctcgc gtgccgcggc cgcctgcgag ctgacacgga agtccgcctg gccgaagagc
     2041 gctcctggtc gcccgcgggc gagcggctca gcgctctctt tcccgcggcg cagccttc
//
LOCUS       DASRJB010000777         7278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4176182, whole genome shotgun sequence.
ACCESSION   DASRJB010000777 DASRJB010000000
VERSION     DASRJB010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4176182"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFB80_20495"
     CDS             <1..493
                     /locus_tag="VFB80_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36226.1"
                     /translation="SPLALYRSGPRQWGQWQLESFDAAARQRLRSGANFPAAASPPSS
                     KAPASEAQVPIFASHDDFDSAERPDLGRALRLLADRARVANPAGHALIRVILRRGHAA
                     EEQWLKVIRVRPQKDGNDEFLAEVVDDSQLWPFLRAGERVRVPIYEPLDVRETSPASA
                     PSK"
     gene            complement(439..1497)
                     /locus_tag="VFB80_20500"
     CDS             complement(439..1497)
                     /locus_tag="VFB80_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36227.1"
                     /translation="MKLRVGIIGLGEAWDARHRPALRALSDRFEVKAVCCEVAHLAEQ
                     AARDFAACEVDGFRALCAREDVDVILILAPEWYGPLPVVAACDFGKSVYCGAALDFDD
                     PAQAQSLRERVERAGIAFMAEFPRRLAPATLRLKELIATRLGQPKLLFCHRRSPIDPG
                     APPQRRGRNCPTMLREMIEQVDWCRYVIGQNPTSVVGIEHGNIDTSDGDDYRMMSLDY
                     SAAGKLGEGPIAQISCGRYMPGAWPEAASFRPPAALQVCCERGIAFIDLPATLVWFDE
                     AGRHMEALDSERPVGERLLMHFYRAVTSLVRRSDDLEDAYRALATVLAARQSFDTGRR
                     IYLDGAEAGEVSRTSSGS"
     gene            1654..2334
                     /locus_tag="VFB80_20505"
     CDS             1654..2334
                     /locus_tag="VFB80_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN36228.1"
                     /translation="MLRLSVVIPALNEAPLVGRAVSSAWEAGADEVLVADGGSSDGTP
                     RAAACSAQVIAAPRGRAAQQNAAARLASGDVLLFLHADNWLAPDAGRQIRAALAAGPA
                     THGALWQRIEAAGWCYRWLERGNAARVRWCGVPYGDQAIFIRRAVFEAAGGWPEAPLL
                     EDVILMRRLRRASWPLLLPGPVFVSPRRWQRHGVIRQTLRNWRLLAAFALGASPERLA
                     AQYRRHDA"
     gene            complement(2355..3710)
                     /locus_tag="VFB80_20510"
     CDS             complement(2355..3710)
                     /locus_tag="VFB80_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN36229.1"
                     /translation="MHEPRLRPDRCASAHRLALWGILLAVAATAGGSEFRHSPIVKAV
                     QEAEGAVVNIHGRKTVRAENASYGTPEGLRQVNGMGTGIVIDRRGYVLTNYHVVEGVT
                     NIQVSLSDGKSTVARLVSHDPKTDLAVIKIDGKPLPVIRCGTSCDLMKGEPVIAIGNA
                     FGYEHTVTDGIISALHRNVQVSDEQKYNDLIQSDASINPGNSGGPLLNIDGEVIGINV
                     AVRVGAQGIGFAIPIDEALEIAARLMSTERLEQTSHGIVGKTVCEPNRRQFVVSTARD
                     SSPAGRAGLKSGDVITAIGGHKVERSLDIELALLGQPVGSELEVEVHRNGQTLPLQLA
                     LSPLSRTTPKPALADRSWTTLGLRLAVMDAAEFNRLGTRYRGGLTVLEVRPNSPAAIQ
                     GIRRGDVLVGMHVWETVSLENIAYILDRDDLTKLDSVVFYIVRGSDTLFGHLRLAQRT
                     P"
     gene            complement(3901..4308)
                     /locus_tag="VFB80_20515"
     CDS             complement(3901..4308)
                     /locus_tag="VFB80_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36230.1"
                     /translation="MSLAKLLLSPLALALLALGGCCCCPPLSGRYSEIGQAPHSASDA
                     CGCGGNGGGLGHVPLVAGLHHGSGGLGNKGLNLAPSQQHADYVSPLARFHPVPTRPVF
                     EPLPAYHPPVLLDPLPTRQWIAESGKLSVANAH"
     gene            complement(4406..5683)
                     /locus_tag="VFB80_20520"
     CDS             complement(4406..5683)
                     /locus_tag="VFB80_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36231.1"
                     /translation="MKLALLGIDDDALDLVRWAVAGGGHELVAAYDVGSRAAEVRALA
                     PRAQLNESWEALVLGSMAEAVIVGRGGAELAGETGIADSERRAEQLRKLVQAAVPLLV
                     VCPACESIVGFEIEMIRRDAKAVIVPYVSQAEHPAIRSLAEIVSWGESSPLGAVEQIS
                     LEREQADRSRPAVLEQLSRDLAAIRQLIGTIHSVSASGPAASLGRDPLGPKLKELPSL
                     ANLSVHVVGDEGLSARWSIGPALARPSGRLTVTGQRGKAVLQMPNGEDWSLEVGGDNL
                     ARESFAARPDPAGMFRKLSHAISTEDFKDDEAWLAAARDQEAAEAVDRSLARGRTIEL
                     LGEEHTEEDSFKGVMAMGGCLLLALAVCTVFVATIVEGLRLPLRNWPVWKLWPVYLLA
                     PIVVFLLLQLFQLAIKRETPDLRRLVGSEDSPR"
     gene            complement(5817..6596)
                     /locus_tag="VFB80_20525"
     CDS             complement(5817..6596)
                     /locus_tag="VFB80_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HZN36232.1"
                     /translation="MSVTQPAAIVTGASRGIGRAIAIRLASDGFAVTVNYHSSAAAAA
                     EVVGEIEQAGGKALAVQADVGAAGDRQALVDRTLQQFGRLDLLVNNAGITSVGRKDLL
                     EATEESWDTVFATNLKGPFFLAQAAARAMISQVQSGTIGRGTIVNVSSISAYAVSTNR
                     ADYCLAKAAMPMMTWLLADRLAEHRINVYEVCPGVIASDMTAPVKEKYDKLIAEGLSP
                     IRRWGQPEDVAAAVAMLAAGQLPFSTGERINVDGGFHIRRL"
     gene            complement(6609..>7278)
                     /locus_tag="VFB80_20530"
     CDS             complement(6609..>7278)
                     /locus_tag="VFB80_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36233.1"
                     /translation="ALLNCGFRLMPTAGTASGVHPVPLGWGRVYVNCPNGFTYHDWMK
                     GLAEGRSFVTTGPLMRVYVNEQPAQPKYKQSGEDVHYYISADVWSHWRNVRWDVLMGG
                     RRVSWGDGSAAAGRNEGRAPGSRDSPTTRFGAGAKVSSSTWFVVRCWETLPDGRERFA
                     HSAPIFIDVEGKPLRPRKAEVEYLVSRVRSEIERNTGVLSEEALAEYREALAAYESIA
                     KTAQ"
BASE COUNT         1174 a         2464 c         2456 g         1184 t
ORIGIN      
        1 gagtcccctg gcgctctatc gctccgggcc gcggcagtgg gggcagtggc agctggagtc
       61 gttcgacgcc gcggcgcggc agcggctgag gtcaggcgcc aatttccccg ccgcggcttc
      121 gccgccgtcc agcaaggcgc ctgcgagcga ggcccaggtg ccaattttcg ccagccacga
      181 cgacttcgac tccgcagagc ggccggacct gggccgcgct ctgcggctgc tggccgaccg
      241 ggcacgggtc gccaatccgg ccggccacgc gctgattcgc gtcatcctgc ggcgcgggca
      301 tgccgcggaa gagcagtggt tgaaggtaat tcgcgtccgg ccacaaaagg acggcaacga
      361 cgagttcctg gcggaagtag tggacgacag ccaattgtgg ccgttcttgc gggccggcga
      421 gcgagtgcgc gtgccgatct acgagccgct cgacgtccgc gagacttcgc ccgcttccgc
      481 gccgtccaag tagatgcggc ggccggtatc gaagctttgc cgggcggcca gcaccgtggc
      541 cagcgcccga taggcgtcct ccaggtcatc ggaccgccgc acgaggctgg tgaccgcgcg
      601 gtagaagtgc atcagcagtc gttcgccgac ggggcgttcg ctgtcgagcg cttccatgtg
      661 ccggccggcc tcgtcgaacc agaccagcgt ggcgggcagg tcgatgaacg cgatgccccg
      721 ttcgcagcag acctgcaggg ccgccggcgg ccggaacgac gcggcttccg gccacgcgcc
      781 cggcatgtag cggccgcagc tgatttgagc gatcggccct tcgcccagct tgccggcggc
      841 ggagtagtcc aggctcatca tccgatagtc gtcgccgtcg gaagtgtcga tgttgccgtg
      901 ctcgatgccg acgaccgatg tggggttctg gccaatgacg tagcggcacc agtcaacctg
      961 ttcgatcatc tcgcggagca tcgtcgggca gtttcgcccg cggcgctgcg gtggggctcc
     1021 gggatcgatc ggagagcggc gatgacaaaa cagcagcttc ggctggccca gccgcgtggc
     1081 gataagctcc ttcagccgca gcgtcgcggg cgcgagccgg cggggaaatt cggccatgaa
     1141 ggcgatgccg gcccgctcga cgcgctcccg gaggctttgg gcttgcgcgg ggtcgtcgaa
     1201 atcgagggcc gctccgcagt aaacgctttt gccgaagtcg caggccgcca cgaccggcag
     1261 cgggccgtac cactcggggg cgagaatcag aatgacatcg acgtcctcgc gggcgcacag
     1321 cgcgcgaaac ccatcgacct cgcaggcggc gaagtcgcgg gccgcctgtt cggccaggtg
     1381 ggccacctcg cagcacaccg ccttgacctc gaaccggtcg gaaagcgccc gcagcgccgg
     1441 gcgatgccgc gcgtcccaag cttcgcccaa tccaataatg ccgacgcgaa gtttcatgcc
     1501 tggcccaaag agattcgaag acgagcgcca aattatatcg gcagctagtg ccggtgtctg
     1561 ccatcgcaga cgagagtacc ttgtcaggct gtagccgaat tcgtcagaat tcggacgaaa
     1621 aaccggacca atttctggcg aaatcggcta ggcatgttgc ggctgtccgt ggtcattccg
     1681 gctctaaacg aagcgccgct cgtcggccgc gcggtgagtt cggcgtggga agccggcgcg
     1741 gatgaagtgc tcgtcgccga cggcggcagc agcgatggca cgccacgagc ggcggcgtgc
     1801 agcgcacagg tcatcgctgc gccgcgcggc cgcgcggcgc agcagaacgc ggccgcccgc
     1861 ctggcgagcg gcgacgtgct gctgtttttg cacgccgaca attggcttgc gcccgatgcc
     1921 ggccggcaaa tccgcgcggc attggccgcg gggcccgcga cgcatggcgc tctgtggcag
     1981 cggatcgaag cggcgggctg gtgctatcgc tggctggagc gcggcaatgc ggcccgcgtc
     2041 cgctggtgcg gcgtgccgta cggcgatcag gcgatcttca tccgccgcgc ggtcttcgaa
     2101 gccgccggcg gctggccgga agcgccgctc ttggaagacg tgatcctgat gcggcgactg
     2161 cggcgagcgt cgtggccgct gctgctgccg ggacccgttt tcgtgagccc gcggcgctgg
     2221 cagcgccacg gcgtcatccg gcaaacgcta cgcaactggc gattgctcgc ggcctttgca
     2281 ctgggcgcga gccccgagcg gctcgccgca caataccgcc gccacgatgc gtgagccgta
     2341 gcgtgaaagg atcgttacgg cgtccgctgc gctagccgca ggtggccgaa gagcgtgtcc
     2401 gatccgcgga cgatatagaa cacgaccgag tcgagcttcg tcaggtcgtc gcggtcgagg
     2461 atgtaggcga tgttctcgag cgagaccgtt tcccagacgt gcatcccgac cagcacgtcg
     2521 ccgcggcgga tgccttgaat ggccgcgggg ctgttgggcc gcacctcgag caccgtcaag
     2581 ccgcctcggt agcgcgtgcc caggcggttg aactcggctg cgtccatcac ggccagccgc
     2641 aagcccagcg tggtccacga ccgatcggcc aaggccggct ttggcgtggt gcgcgagagg
     2701 gggctgaggg ccagctgcag gggcagcgtc tggccgttgc ggtgcacttc gacctccagc
     2761 tcgctgccca ccggctggcc gagcagcgcc agctcgatgt ccaggctgcg ctcgaccttg
     2821 tgtccgccga tcgcggtgat cacgtcgccg ctcttcaggc cggcgcgacc cgccgggctg
     2881 ctgtcgcggg cggtggagac gacgaattgc cgccggttgg gctcgcagac cgtcttgccg
     2941 acgatgccgt ggctggtctg ctccagccgc tcggtgctca tcaagcgggc ggcgatttcc
     3001 aaggcctcgt cgatcgggat ggcgaagccg atgccctgcg cgccgacgcg gacggcgacg
     3061 ttgatgccga tcacttcgcc gtcgatgttg agcagcggtc cgccggagtt gccgggattg
     3121 atgctggcgt cgctctgaat caggtcgttg tatttctgct cgtcgctgac ctgcacgttg
     3181 cggtgcaggg cgctaatgat gccgtcggtc accgtatgct cgtagccgaa ggcgttgccg
     3241 atggcgatca ccggctcccc cttcatcagg tcgcacgacg tgccgcagcg aatcaccggc
     3301 agcggcttgc cgtcgatctt gatcacggcg aggtcggtct tgggatcgtg cgagacgagc
     3361 cgggcgacgg tcgatttgcc gtcggacagc gagacttgaa tgttggtcac gccttcgacg
     3421 acgtggtagt tggtgagcac gtaaccgcgg cggtcgatga cgatgcccgt gcccatgccg
     3481 ttaacctggc ggaggccttc gggcgtgccg tagctggcgt tctcggcccg cacggtcttg
     3541 cggccgtgga tgttgacgac ggccccttcc gcttcctgca ccgccttgac gattggcgag
     3601 tggcggaatt ccgacccgcc ggccgtcgca gcgaccgcca gcaggatgcc ccacagggcc
     3661 agacggtggg cggacgcgca gcgatcgggg cgcaggcggg gctcgtgcat gagttgctcg
     3721 tcaggcctgg gtcgtcgagc gagtcggccc gcaggtagcg gggtagcctg caagccgagc
     3781 gcgatgggca atccgatggt ttgatcggac cgttcgccgc atccgcatca cggggggcca
     3841 tagcgacggc agactcgccg caccttgcca cgcgccggaa caaccggcaa agatgcccgt
     3901 ctaatgggcg tttgccacgg aaagcttgcc gctttcggcg atccactggc gggtgggcag
     3961 gggatcgagc agcacgggcg gatgataggc cggcagcggc tcaaacaccg gccgcgtcgg
     4021 caccggatgg aatctcgcca gcgggctgac gtagtcggcg tgctgctgcg acggagccag
     4081 gttcagtccc ttgttcccca gtccgccgct gccgtgatgc agccccgcca cgagcggtac
     4141 atggccgagc ccgccgccat tgccgccaca tccgcaagca tcgctggcgc tatgtggcgc
     4201 ctggccgatc tcggaatacc ggccggagag cggcggacag cagcagcagc cgcccagcgc
     4261 cagtagcgcc agcgctagcg gcgaaagcag cagcttggct agcgacatga tgcccccatc
     4321 agctcgtgag tattcccgcg ctgcggactc atcggagcgc cgcgccgtca aacttgagct
     4381 atccggcaga atcgccgcgg cgggattacc ttggcgagtc ttcgctgcca accagccgcc
     4441 gcaggtcggg cgtctcgcgc ttgatggcca actgaaagag ctggagcagc aggaatacga
     4501 cgatcggcgc gagcaggtag accggccaga gcttccacac cggccagttc cgcagcggca
     4561 gccgcaggcc ctcgacaatc gtcgccacga acaccgtgca gacggccagc gccaagagca
     4621 ggcagccgcc catggccatg acgcccttga acgagtcctc ctcggtgtgt tcctcgccga
     4681 gcagctcgat cgtccgcccg cgggccaggc tgcgatcgac ggcctcggcg gcctcctggt
     4741 cgcgggccgc tgcgagccag gcctcgtcgt ctttgaagtc ctcggtcgag atcgcgtgcg
     4801 agagttttcg gaacatacca gccgggtcgg gccgggcggc gaacgattca cgagccaggt
     4861 tgtcgccgcc gacttcgagc gaccagtcct cgccattggg catctggagc acggctttgc
     4921 cgcgctggcc ggtgacggtg agccggccgc tgggacgcgc cagcgccggc ccgatcgacc
     4981 accgcgcact gagcccttcg tcgccgacga cgtgcacgct caaattggcc agggacggca
     5041 actccttgag cttgggacca agcggatcgc ggccgaggct ggccgccggc ccggaagccg
     5101 acacgctgtg gatggtaccg atgagctgcc gaatcgccgc caggtcgcga gagagctgct
     5161 cgagcacggc gggccggctg cggtcagcct gctcgcgctc cagcgagatt tgttcaactg
     5221 cacccagcgg cgaagattcg ccccacgaga cgatctctgc cagcgagcgg atggccggat
     5281 gttcggcctg cgagacatac ggcacgatca ctgccttggc gtcgcggcgg atcatttcga
     5341 tctcgaaccc gacgatcgat tcgcaggcgg ggcagaccac cagcagcggc acggcggctt
     5401 gaacgagctt gcggagttgc tccgcgcggc gctcggagtc ggcgatgccc gtttcgccgg
     5461 ccagttccgc tccgccgcgg ccaacgatga cggcctcggc catggagccg agcaccagcg
     5521 cttcccagct ctcgttcagc tgcgcgcgcg gagcgagtgc gcggacttcc gccgctcgcg
     5581 agccgacatc ataagccgcg accagctcgt gcccgccgcc cgccacggcc cagcggacca
     5641 aatccagcgc gtcgtcgtcg atgcccagca gcgcgagctt catacagcca ttgtatcgcg
     5701 atacagtccg gcgggagatt cgccagcaga tgtagggtgt gacaagcgag tctgcgagcg
     5761 ccggcacacc ctccgtttcc ttggtgtgcc gacgcccgcg gcgggcttgt cacaccctac
     5821 agccgccgga tgtgaaaccc gccatcgacg ttgatccgct caccggtgct gaacggcaat
     5881 tgaccggcgg cgagcatcgc gaccgcagcc gcgacgtctt ccggctggcc ccagcggcgg
     5941 atcggcgaga ggccctcggc gatcagcttg tcgtatttct ccttgaccgg cgcagtcata
     6001 tcgctggcga tcacgcccgg gcagacctca taaacgttga tccggtgctc agccagccga
     6061 tcggccagca gccaagtcat catcggcatc gccgccttgg ccaggcagta gtccgcccgg
     6121 ttggtcgata cggcatacgc cgagatcgac gacacattga cgatcgtccc gcggccgatc
     6181 gtgccgctct ggacttgcga gatcatcgcg cgggcggccg cctgcgccag aaagaaggga
     6241 cctttgagat tcgtcgcgaa gaccgtgtcc cagctttcct ccgttgcttc cagcaagtct
     6301 ttgcggccga cggaggttat ccccgcattg ttgacgagca aatcgagccg gccgaattgc
     6361 tgcagcgttc gatcgaccag cgcctggcga tccccggccg cgcctacgtc ggcctggacc
     6421 gccagcgctt tgccgcccgc ctgctcgatc tcgccgacaa cctcggcagc cgccgcggcg
     6481 ctcgaatggt agttgacggt tacggcgaaa ccatcactcg ccaggcgaat cgcaattgcc
     6541 cgcccgattc cgcgggaggc gccggtgacg atcgctgccg gttgagtcac tgacacattg
     6601 cccccgccct actgtgccgt cttggcgatg gactcgtacg cggccaaggc ttcgcggtat
     6661 tccgccagcg cctcttccga caggacgccc gtattccgct cgatctcgct gcgcacgcgg
     6721 ctgacgaggt actcgacctc cgctttccgc ggccgcagcg gcttgccttc cacgtcgatg
     6781 aagatcggcg cggagtgagc aaatcgctcg cggccgtcgg gcagcgtctc ccagcagcgg
     6841 acgacaaacc acgtggagga agaaaccttc gcgcctgcgc caaatctcgt ggttggagag
     6901 tcgcgactgc ctggagctcg accttcgttt cttcctgcag ctgccgaccc atctccccac
     6961 gacacacgtc gtccgcccat cagcacgtcc cagcggacgt tccgccaatg cgaccagaca
     7021 tccgcggaaa tgtagtaatg gacgtcctcg ccagactgct tgtacttcgg ctgagccggc
     7081 tgttcgttga catagacgcg catcagcggc ccggtggtca caaaactccg cccttcggcc
     7141 agccccttca tccagtcgtg atacgtgaag ccgttagggc aattcacata gacccgcccc
     7201 caacccagcg gcaccggatg cacacccgac gccgtgcccg ccgtcggcat caggcggaag
     7261 ccgcagttga gcagggcg
//
LOCUS       DASRJB010000778        10580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3892405, whole genome shotgun sequence.
ACCESSION   DASRJB010000778 DASRJB010000000
VERSION     DASRJB010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3892405"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFB80_20535"
     CDS             <1..247
                     /locus_tag="VFB80_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN36234.1"
                     /translation="WAPWCAPCRRIAPMIDELASENLGAAVIGKVNIDDNQQSAMTYG
                     VEAIPTIIIFKGGQPVQRFQGIPSKARVQEALDAAKA"
     gene            complement(316..1080)
                     /locus_tag="VFB80_20540"
     CDS             complement(316..1080)
                     /locus_tag="VFB80_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36235.1"
                     /translation="MPKPHWLVTFVVFSASSACIAADEWGDLKLRFSYDGEPPKPRLM
                     KVAPDRLVPDESLIVEPKDLGIANVCVWLSTEDGASPPVHPDSAVAGNDKFAGTIRDG
                     RFSPHIMIVRPPQGFLVRNHDHVGYDTNVEFSRNGSLSSTLKPDATFAVFLDREEDYP
                     ARLTCSIHPSLSGYVLVRSTPYSLVSDESGKVSFENLPVGKWTFVVWHERAGFVKQAA
                     RAGKVVEWPRGRATLEIKPGENDLGEIKLSPELFAR"
     gene            complement(1091..1873)
                     /locus_tag="VFB80_20545"
     CDS             complement(1091..1873)
                     /locus_tag="VFB80_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HZN36236.1"
                     /translation="MARWPAVIVVAWLFPASLPAAEWGNLTMRFVYDGMPPVAKPIAA
                     AAGPAGAGCGKGPFVEERLVVNPKDKGIANVCVWLLRTSSEPAPAIHESYAKTAKDKV
                     TMTNKACRFEPRILVVRTSQTLVGANGDPTGHNMKASFFNNPEFNENIPAGAQILKSL
                     AKPESAPSPVNCGMHPWMGGYLIVRDDPYASVSDASGKVTLANLPAGQWTFVVWHESG
                     YVTKAKQGGQAQDWGRNGRVKITIQPGDNDRGEFLLPPDEFP"
     gene            complement(2065..3765)
                     /locus_tag="VFB80_20550"
     CDS             complement(2065..3765)
                     /locus_tag="VFB80_20550"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HZN36237.1"
                     /translation="MADEAAAKKAAPKKDAGKKEKKSAKEGGGGWLARLMALPKMISA
                     AGCASCLTSVFLMLLVIGVWLVFYFEPGNLPWRHAMGFWTRIVPTLLLVVLIPLVLYR
                     AIRLWLQGEQSAHPDIDFAWKAGMEALAQNGLSPGSVPLFLVLGSASVRQEMALVRAA
                     GLSLRVHDVPEGPAPLHWYASPDGIYLVLSEACSLSSLATLLEKRRAQAPSLGESSTP
                     GDLPALPAPGIAAPRPAPKPAPAVKRERPAPGGDRTRGTIMLDQFVSDRQEAEAQAQA
                     AATATEDEAEAAAPAAMTVSDQPAILSQQESARQKSRLEYVCHLIRQARFPICPINGV
                     MVLLPFELLQATQRETQQLQKTVRADLGCLQRELEIRCPVTALIVGMHQERGFRELVR
                     RVGRERATAQRFGRRFDVRIIPTTDNLAALCSLSAGVFEDWIHTLFREAEALTRPGNL
                     RLYGLLCKVRSTLSGVLTEVLVGGFAHDPQNKEVAEPIPFSGCYFAATGETEDQQAFV
                     RGVFEKLVEEQEEIEWTRLAAGDNRLYILVALAAIAATAVCLIVIAVRIGLGVWWSLH
                     "
     gene            3973..4905
                     /locus_tag="VFB80_20555"
     CDS             3973..4905
                     /locus_tag="VFB80_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN36238.1"
                     /translation="MLNRAWLVLLVLLVAASWAAGQDAPRKLVLVAGKPSHPPRMHEF
                     NAGVQLLGKCLRDVPGLKTEIVLGGWPKDEKVFDGAAAVVFYMDGGAKHEAVQEGRRR
                     LDLIDGWTKKGIGIGCMHYGVEVVPDQAGKEFLRWIGGHYEHMYSANPIWEPKFAALP
                     EHPITRGVQPFAAKDEWYFNMRFVGGTAGNKPSESAGLKFTPILVATPSDDVRDGPYV
                     YPPGPYPHIQADKGRAECMLWAVERPDGGRGFGFTGGHFHDNWGNDNFRKTILNALVW
                     VAKVEVPSGGVESRVTPADLDANLDPKPQPKVKR"
     gene            complement(4909..5097)
                     /locus_tag="VFB80_20560"
                     /pseudo
     CDS             complement(4909..5097)
                     /locus_tag="VFB80_20560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(5195..6334)
                     /locus_tag="VFB80_20565"
     CDS             complement(5195..6334)
                     /locus_tag="VFB80_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36239.1"
                     /translation="MPSLPLNRRLPTTVLAFRGYNVTNLGRTAELLAHPAYGSTVESH
                     LRGVSGIAADLLRRPVDLVDRVRRGEETTLATYGDAVALLIGVQAAQLELLGKFFGVD
                     YRGAQFSFGYSLGEIAALVAGGLLDPAAALQVPLSLADDCVALADGVTLAVFFTRSQA
                     IDFGLVERLCLEVNQAGRGVMGISSYLAPNTVLLLGQGDTLERFLERAKTLFAVRVHL
                     RKNDHQWPPLHTPIVWQRQISNRAAQRMQTLPIKLVEPKPRVFSLVTAGYSYTPTSAR
                     EILNRWVDHPQRLWDGIYETLASGASTVIHLGPDPNIIPATFRRLSEDVASQLQDSRS
                     LRALSAAVRRLWLRRILPQRAALLRAPYIEHIILEDWLLAHTPPK"
     gene            6674..7867
                     /locus_tag="VFB80_20570"
     CDS             6674..7867
                     /locus_tag="VFB80_20570"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36240.1"
                     /translation="MRKRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMQCGNH
                     LKQIGLGLQNYHDVFSSLPYGARSRQGADPAVTGRTNFWGPSWYVGLLPFCEQKNLAD
                     QIEQFSIIGNAANTGVDFNSTNGTAGTVSAVSHNQKIPWMLCPSSPLPQTETVGSALL
                     TTPSYVGIAGAVQSATPGDGVAALQSTQSVATETAFIETRAMPSGSAAPGPNAGYVSG
                     GGLLVINECFNMAADIDGTSSTIVVSESSDYFYLQANATATYVRVRVDGGAISSGGPG
                     GRWFQGTSNAFKAPATNTVAAGSVLNLTTLRAFPNPAGACIGFNGKGALIANLGQPIT
                     GIGTATTTAAGNIGNNRPLLSAHPNVVLAVFMDGHTQALTKTTHAAIVKRLSTRDDGQ
                     NITDF"
     gene            8060..9232
                     /locus_tag="VFB80_20575"
     CDS             8060..9232
                     /locus_tag="VFB80_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZN36241.1"
                     /translation="MPQQRKFVTQSPENSETPLADVHGWVTPTRLFFVRNHFDVPAIE
                     QKSWRLAVGGRVARPLELNWDDLDAMPQRTLLATLECAGNGRSFLAKKAAGVPWGAGA
                     VAHAEWSGVTLGSVLDLAGIQSDAVEIVCMGADRGTEPDHPEPMHFARGLPLAKARHR
                     DTLVALRMNGEPLTPSHGFPARLLVPGWYGVASVKWLTRLEASPVPFEGYFQTVKYTV
                     QRAGASGPETVGIQGMAVKSEIVRPFAGEALGLGKQRVFGLAWAGEEAVAKVELSTDG
                     GSTWREAELLGPSAPYSWIMWEYLWDVRTPGFSRLVSRAISTSGRVQPAEHDSLLGGY
                     LIHFSRPIPVRVEPRAAADAASGDVDVLRYDMNAFAESNARLPLDVDLEYSLGAGI"
     gene            9268..9807
                     /locus_tag="VFB80_20580"
     CDS             9268..9807
                     /locus_tag="VFB80_20580"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HZN36242.1"
                     /translation="MPRPPIAALAELDPYAGRIPLAALSQWLARTEVTLKDVQPFLRF
                     SPEHYIRNLMYAGPGYQALVLCWRSGQRSPIHDHTGSSCGVKVIRGVATETTFAAAPN
                     GMVYALGSRHLPAGSTCASQDADMHQMSNLQPEGEDLITLHIYSPALVYMNMYSIVAA
                     SVERFFDPINDEFVSGAGI"
     gene            9827..10207
                     /locus_tag="VFB80_20585"
     CDS             9827..10207
                     /locus_tag="VFB80_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HZN36243.1"
                     /translation="MASSAVVRTVLLLIASNVFMTFAWYGHLRGLEKQPWLVAALVSW
                     GIALLEYLLQVPANRIGALEAHLQVGQLKIMQEVITLSVFVPFSIWFLKEPIKLDYLW
                     AALCLCGAVFFVFRGELTAAALGK"
     gene            10210..>10580
                     /locus_tag="VFB80_20590"
     CDS             10210..>10580
                     /locus_tag="VFB80_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36244.1"
                     /translation="MHAGEATTRTFAPLLERPADGEKSPPRGAPPLVRLRWVIAAILV
                     GLAAFSALRLWFVEGLLRPATIDGPSMAPLLLPAHFAVTCGDCGFRFACDAERPPPAG
                     QAVCPNCGYAGNDLARAKARP"
BASE COUNT         1814 a         3407 c         3475 g         1884 t
ORIGIN      
        1 ctgggccccg tggtgcgccc cgtgccgccg aattgccccg atgatcgacg agttggcttc
       61 cgaaaacctc ggcgcggccg tgatcggcaa ggtcaacatc gacgacaatc agcaatcggc
      121 gatgacctac ggggtcgagg cgattcccac gatcatcatt ttcaagggcg gccagccggt
      181 gcagcgcttc cagggcattc ccagcaaggc ccgcgtccag gaagccctcg acgccgccaa
      241 ggcctgacga cgctggcagc gtcgtcaacc gtagctgacg ctgccagcgt cagcgcgcat
      301 cgccaatgcc gtggctcagc gagcaaacag ctcaggcgac aacttgattt cgcccaggtc
      361 gttttcgccc ggcttgattt ccaacgttgc tcgcccgcgc ggccattcaa ctaccttgcc
      421 ggcgcgagcc gcctgcttga cgaagcccgc ccgctcgtgc cagaccacaa aggtccattt
      481 gcccacgggc agattctcga atgagacctt gccgctctca tcgctgacga gcgaataggg
      541 tgtgctgcgg acgagaacgt agccgctcag cgacggatga atggaacacg tcaggcgggc
      601 agggtaatcc tcttctcgat ccaaaaacac ggcaaacgtc gcatccggct tcagggtcga
      661 actcagcgag ccgttgcgtg aaaactcgac attcgtgtcg taaccaacat ggtcatggtt
      721 cctcaccagg aagccctgcg gcggccgaac gatcatgatg tgcgggctga agcggccgtc
      781 gcgaatcgta ccggcgaact tgtcgttgcc tgccactgcg gaatcgggat gaaccggcgg
      841 gctcgcgcca tcttcggtgc tcagccatac gcaaacattg gcgatgccca ggtcttttgg
      901 ctcaacaatc agcgattcgt ccggaaccag gcgatcgggc gcgactttca tcagccgcgg
      961 cttgggcggt tcgccgtcat agctgaaccg cagcttgaga tcgccccatt cgtcggcggc
     1021 aatgcaagcc gaggacgccg agaaaaccac gaacgtcacc agccagtgtg gtttgggcat
     1081 gactcagttc ctacggaaac tcgtccggcg gcagcaggaa ctctccgcgg tcgttgtcgc
     1141 cgggctggat cgtgatcttc acgcggccgt tgcggcccca gtcctgagct tggccgcctt
     1201 gcttcgcctt ggtgacgtag ccgctctcgt gccagacgac gaaggtccac tggccggcag
     1261 gcagattcgc cagcgttacc ttcccgctgg cgtcgctgac agacgcgtag ggatcgtcgc
     1321 gaacgatcaa gtagccgccc atccagggat gcatgccgca gttcaccggc gacggcgcac
     1381 tttccggttt cgccagcgac ttcaggatct gtgcgccggc aggaatgttc tcattgaact
     1441 cgggattatt gaagaacgag gccttcatgt tgtgcccggt gggatcgcca ttcgcgccga
     1501 ccagcgtctg ggacgtgcgg accacgagaa tccgcggctc gaagcggcaa gccttgttag
     1561 tcatcgtgac cttgtccttg gccgtcttgg cgtacgactc gtggatcgcg ggcgcgggct
     1621 cgctgctggt gcgcagcagc cagacgcaga cgttggcgat gcccttgtcc ttcgggttca
     1681 ctaccaagcg ctcttcgaca aacggcccct tcccgcagcc cgcgcccgcg ggtcccgctg
     1741 cggccgcgat cggcttggcc accggcggca tgccgtcata gacgaaccgc atcgtgaggt
     1801 tgccccactc ggcggccggc agcgaagcgg ggaacagcca agcgacaacg atgacagccg
     1861 gccagcgtgc catggcagcg agccccccgc cgcccatcct actcctgctc ttactcctgc
     1921 tcctaatcat cttcctaatc ttaatcctca tcctgatcat tctctttccc cgcccgccgc
     1981 cgggagcaag agtaagagga agattaggat taggaatagg attaagagca ggatgaagat
     2041 taagagcagg attcaccgca cgattcagtg caggctccac catactccca gcccgatgcg
     2101 aacggcgatc acgatcaggc agacggccgt ggcggcgata gctgcgaggg cgacgaggat
     2161 atagaggcga ttgtcgccgg cagctagccg cgtccactcg atctcttcct gctcctcgac
     2221 gagcttctcg aagacgccgc ggacgaacgc ctgctggtct tccgtctcgc cggtcgccgc
     2281 gaaatagcag ccgctgaacg ggatcggctc ggccacttcc ttgttctgcg gatcgtgggc
     2341 aaagccgccg accagcacct cggtgagcac gcccgagagc gtcgagcgga ccttgcagag
     2401 caggccgtac agccgcaggt tgccggggcg ggtgagagcc tcggcctcgc gaaacagcgt
     2461 atgaatccag tcctcgaaca cgccggccga gagcgagcag agggcggcga ggttgtccgt
     2521 cgtcgggatg atccgcacgt cgaagcggcg gccgaaccgc tgggccgtcg cccgttcgcg
     2581 gccgacgcga cggaccagct cgcggaagcc gcgctcctgg tgcatgccga cgatcagggc
     2641 cgtcaccggg cagcggattt ccagctcccg ctgcaggcag cccaggtcgg cccgcaccgt
     2701 cttttgcagc tgctgcgtct cgcgctgcgt cgcctgcagc agctcgaacg gcagcagcac
     2761 catgacgccg ttgatcgggc agatcgggaa gcgggcctgg cggatcagat ggcagacata
     2821 ttcgagccgc gatttctgcc gggccgattc ctgctgcgag aggatcgccg gctgatcgct
     2881 gacggtcatc gcagcggggg cggcggcctc ggcttcgtct tccgtggccg tggcggcggc
     2941 ctgcgcctgg gcctcggcct cctggcggtc ggagacgaac tggtcgagca tgatcgtgcc
     3001 gcgggtgcgg tcgccgccgg gcgcggggcg ctcgcgcttg accgccggcg cgggcttggg
     3061 cgcaggccgc ggcgctgcga ttcccggcgc gggcaacgcg ggcaggtcgc cgggcgtgct
     3121 cgattcgccg agcgagggcg cctgcgcccg ccgcttttcg agcagcgtcg cgaggctgga
     3181 gaggctgcag gcttcggaga gcacgaggta gatgccgtcg ggactggcgt accagtggag
     3241 cggggcggga ccttcgggca cgtcgtgcac gcgcagcgac aggccggcgg cccgcacaag
     3301 ggccatttcc tgccgcacgc tcgccgagcc cagcaccagg aacaggggca cgctgccggg
     3361 cgacaggccg ttctgggcca gcgcctccat gcccgccttc caggcaaagt cgatgtcggg
     3421 atgggccgac tgctcgcctt gcagccagag gcggatcgcg cggtagagca cgagcggaat
     3481 cagcacgacg agcagcaggg ttggcacgat tcgcgtccaa aaccccatcg cgtgccgcca
     3541 cggcaggttg cccggctcga aatagaacac gagccagacg ccgatcacca gcagcatcag
     3601 gaagaccgaa gtcaggcacg acgcgcagcc cgcggcgctg atcatcttcg gcagggccat
     3661 caggcgcgcg agccagcccc cgccgccttc cttggcggac ttcttctctt tcttgccggc
     3721 gtcctttttc ggcgcggctt tttttgccgc tgcttcgtcg gccataatcc tgtgcttttt
     3781 cccgccagtg gcagcctgct agcccttgcg gctagttgta gcttgccgcc ccggagaggg
     3841 aaacagcagt cctgtgaaca accccaggtc gccggtaagc gtcgtctgac ttccaccggt
     3901 tgagccggac tcgagggcga tgtccaatct ccagctgaaa gcgccaatcc ttcgtgacga
     3961 aaggacgata cgatgttgaa ccgcgcatgg cttgtgctgc tcgtgctgct ggtcgctgcg
     4021 tcgtgggcag cgggacaaga tgcgccgcga aagctggtac tggtggccgg aaagccgtcg
     4081 catccgccgc ggatgcacga gttcaacgcc ggcgtgcaat tgctgggaaa gtgtttgcgc
     4141 gatgtaccgg gactgaagac cgagatcgtg ctcggcggct ggccgaagga tgagaaggtt
     4201 ttcgatggcg cggcggcggt cgtcttctac atggatggcg gagcgaaaca cgaagcagtg
     4261 caggaaggcc gccggcggct cgacctgatc gacggctgga cgaagaaagg catcggcatc
     4321 ggctgcatgc actatggcgt cgaagtcgta cccgaccagg cgggtaagga gtttctgcgc
     4381 tggatcggcg gccactacga gcacatgtac tcggccaatc cgatctggga gccaaaattc
     4441 gcggcgctgc ccgagcatcc gatcacacgc ggcgtgcagc cgttcgcggc caaggacgag
     4501 tggtatttca acatgcggtt cgtgggcggc acggcaggga acaagccgtc ggagtcggct
     4561 ggcctgaaat tcacgccgat tctggtggcg acgcccagcg acgatgttcg cgacgggccg
     4621 tacgtctatc ccccggggcc gtacccgcac atccaggccg acaaggggcg ggccgagtgc
     4681 atgctgtggg ccgtcgagcg cccggacggc ggccgcggct tcggcttcac cggcggccac
     4741 ttccacgaca actggggcaa tgacaatttc cgcaagacga tcctcaacgc cctggtgtgg
     4801 gtcgccaagg tcgaagtccc ctccggcggc gtcgagtccc gcgtcacgcc cgctgacctg
     4861 gacgccaacc tcgaccccaa gccgcagccg aaggtgaaga ggtagctgct acgcggcctt
     4921 cgcgacgcgg gccagtatgg cgtcttcgct ggggaattcg ccggtttgga gcttgctata
     4981 gatcagctgg ccgtcgaggg tcagctcgaa gcagcctccc gtggagggct ccagctcaaa
     5041 caccgcgatc cgtcgcttga gattggtgag cagcctggcc gccaaactga cggcctcggg
     5101 ttcatagcct cacgacgcgc agtagcggag atgaaatcgc atgaccggac tcctttcctc
     5161 aagtctatgc ctgatatcgg ccagggcaag gcggttactt gggcggcgtg tgcgccagca
     5221 gccaatcttc gagaatgatg tgctcgatgt aaggcgcgcg cagcagggcg gctcgctgcg
     5281 gcaggatcct gcgcagccac agccgccgca cggctgccga aagcgcccgc aggctgcggc
     5341 tgtcctgcag ctggctggcg acgtcctcgc taaggcggcg gaacgtggcg ggaataatgt
     5401 tggggtcggg gccgagatgg atcacggtcg aagctccgct ggccagcgtc tcgtagatgc
     5461 cgtcccacaa gcgctgcgga tggtccaccc agcgattgag gatttcgcgg gcgctggtgg
     5521 gtgtgtagct gtagccggca gtgacgagcg agaagacgcg gggcttcggc tcgacgagct
     5581 tgattgggag ggtttgcatc cgctgtgcgg cgcgattgga aatctgccgc tgccagacga
     5641 tcggcgtgtg cagcggcggc cactggtggt cgtttttccg caggtggaca cggacggcga
     5701 acagggtctt ggcccgctcg aggaatcgct cgagcgtgtc gccctggccc agcagcagca
     5761 ccgtgtttgg cgcgaggtat gaggagattc ccatcacgcc gcggcctgcc tggttcacct
     5821 ccaggcaaag ccgctcgacc agtccgaagt cgatcgcctg gctgcgggtg aagaacacgg
     5881 ccagtgtgac gccgtccgcc aaggccacac agtcgtcggc gagcgagagc ggcacttgca
     5941 gggcggcagc gggatcgagc aacccgccgg cgacgagggc cgcgatctct cccaggctgt
     6001 agccgaacga gaactgcgcc ccgcgataat cgacgccgaa gaatttgccc agcaattcca
     6061 gctgggcagc ctggacgccg atcagcagcg cgacagcatc accataggtt gccagtgtcg
     6121 tttcttcgcc ccggcggacc cgatccacca gatcgaccgg ccgacgtagc aggtcggcag
     6181 cgatgcccga aactccccgc aagtggcttt cgaccgtgct tccatacgcg ggatgggcca
     6241 gcagttcggc cgtccgaccc agattcgtaa cgttgtaccc gcgaaaggcg agaactgtgg
     6301 tcggaagtcg ccggttgagc ggaaggctgg gcattggcca gagtccgatc caaccacaag
     6361 aaagagttag tttttacatc cgtaattatg agatggcagg tgccgtcttt ctcaggatac
     6421 gccacgagag gcaatgttgc ccagcagttg aatctcgaaa ttcctgtcgg ttacttgact
     6481 tttggccgcc cgtctgcgat cttgctgcat tgtagtgcgt cattttgccg tagttactac
     6541 gaaataatgc cgcggaactt gtcgttttat tgggggttta taccgtcagt tttgcgcact
     6601 tcggcgttct taccgcgaca aatttacttg ccgcggtttc atcatctgtt ttgttatcac
     6661 aagggagcct ggtatgagaa agagagcgtt cacgctcgtc gagctgctgg tggtgatcgc
     6721 catcatcggc gtgctcgtgg cgctgctgtt gccggcggtg caagccgccc gcgaagccgc
     6781 ccgccgcatg cagtgcggca accatctgaa gcagatcggc ctgggtctgc agaactatca
     6841 cgacgtgttc agcagcctgc cctacggggc ccgctcgcgg cagggagctg accccgcggt
     6901 gactggccgg acgaacttct ggggcccgag ctggtacgtc ggccttttgc cgttctgcga
     6961 gcagaagaac ctggcagatc agattgagca gttcagcatc atcggcaatg ccgccaacac
     7021 gggcgtcgat ttcaactcga cgaacggcac ggcgggcacc gtttcggcgg tttctcacaa
     7081 ccagaagatt ccctggatgc tttgcccctc cagcccgctg ccgcaaacgg agaccgttgg
     7141 ctcggcactg ctcaccaccc cgtcctacgt cgggattgcc ggcgccgttc agagtgccac
     7201 acccggcgac ggcgtcgcag ccctgcaatc gacgcagtcc gtcgcgacag agacggcgtt
     7261 catcgagacc cgcgccatgc cgagcggatc ggcggctccg ggccccaatg ccggctatgt
     7321 gagcggcggc ggattgctcg tgattaacga gtgcttcaac atggcggccg atatcgacgg
     7381 cacgtccagc acgatcgtgg tgtcggaatc gtccgactac ttctatctgc aagcgaacgc
     7441 gaccgccact tacgtgcgcg tccgggtgga tggcggtgcg atttcgagcg gcggcccggg
     7501 cggccgctgg ttccagggaa cgagcaacgc cttcaaggcg cctgccacca acacggtcgc
     7561 cgccggcagt gtgctcaatc tcaccacgct tcgcgccttt cccaacccgg ccggcgcctg
     7621 catcggcttc aacggcaagg gagccctcat cgccaacctg ggtcagccga tcacgggcat
     7681 cggcaccgcc acgaccacag ccgcgggcaa catcggcaac aaccggccgc tgctctcggc
     7741 gcatccgaac gtggtgctcg cggtgttcat ggacggccac acccaggcgc tgacgaagac
     7801 gactcacgcc gccatcgtga agcgcttgtc cacccgcgac gacggccaga acatcaccga
     7861 tttctagtcg gtctctaagg acctgctgaa acgactatgc agcaccctgg cggcttgccg
     7921 ccagggtgct tttgttttgg cccgcagctg cgctagcctc agcggcccgc ttgccgcaaa
     7981 atgggcctca gaacgccccg gtcttctttc gggcgacagg tttcgacctc gaatcgcgtt
     8041 attacgtcag agggttcgca tgccccagca gcgcaagttc gtcacgcaat cgcccgaaaa
     8101 cagcgaaacg cccctggccg atgtccacgg ctgggtcacg cccacgcggc tgttcttcgt
     8161 ccgcaatcac ttcgatgtgc cggcgatcga gcagaagtcg tggcggctgg ccgtcggcgg
     8221 tcgtgtggcg cggccgctgg aactgaactg ggacgacctt gacgcgatgc cgcagcggac
     8281 gctgctggcg acgctcgagt gcgccggcaa cggccgctcg tttctggcga agaaggccgc
     8341 tggcgtgccc tggggcgcag gcgcggtggc ccacgccgag tggtcgggtg tgacgctggg
     8401 ttcggtgctg gatctggcgg gcatccagtc cgatgcggtc gaaatcgtct gcatgggcgc
     8461 ggaccgcggg acggagcccg atcatccaga gccgatgcac tttgcccgcg gcctgccgct
     8521 ggccaaggcc cgccatcgcg acacactcgt ggccttgcgc atgaacggcg agccgctgac
     8581 gcccagccac ggcttcccgg cgcggctgct ggtgcccggc tggtacggcg tcgcatcggt
     8641 aaagtggctc acgcggctgg aggcttcgcc cgtgccgttc gagggctact ttcaaaccgt
     8701 taagtacacc gtgcagcggg ccggggccag cgggccggag acggtcggca ttcaaggcat
     8761 ggcggtcaag tcggagatcg tgcggccgtt cgccggcgaa gcgctcggcc tgggcaagca
     8821 gcgcgtgttc ggcctggcct gggccggcga ggaggcggtc gccaaagtcg agctttccac
     8881 cgacggcggc tcgacctggc gcgaggcgga actgctcggc ccctccgcgc cgtactcgtg
     8941 gatcatgtgg gagtacttgt gggacgtgcg gacgcccggt ttcagccggc tcgtctcgcg
     9001 ggcgatctcg accagtgggc gcgtgcagcc ggcggagcac gattcgctct tgggcggtta
     9061 cctgatccac ttcagccggc cgatcccggt gcgggtcgaa cccagggcgg cggccgacgc
     9121 tgccagcggc gacgtcgatg tgctgcggta cgacatgaac gcgttcgccg agagcaacgc
     9181 tcgcctgccg ctggacgtcg atctggaata cagcctcgga gcgggaatct gattgacgct
     9241 ttatgctcga gatttggtgc cctacgtatg cctcgaccgc cgatagccgc tctcgccgaa
     9301 ctcgatccgt acgccggccg cattccgctg gccgcgctga gccagtggct cgcgcggacc
     9361 gaagtgacgc tcaaggacgt gcagccgttt ctgcggtttt cgccggagca ttacatccgc
     9421 aacctgatgt acgccgggcc gggctatcag gccctggtcc tttgctggcg gagcgggcag
     9481 cggagcccca tccacgacca caccggctcc agctgcggcg tgaaggtgat ccgcggcgtg
     9541 gcaacggaaa cgaccttcgc cgcggctccc aacggcatgg tttacgccct cggctcgcgg
     9601 cacctgccgg ccggctccac ctgcgcctcg caggacgccg acatgcacca gatgtcgaac
     9661 ctgcagccgg agggcgagga cctgatcacg ctccacattt attcgccggc tctcgtgtac
     9721 atgaacatgt attcgatcgt cgccgccagc gttgagcggt tcttcgatcc gatcaacgac
     9781 gaattcgtca gcggtgcggg catttgacca ttcgcgaggg aacgccgtgg cttcttccgc
     9841 cgtcgtcaga actgttttgc tcctgatcgc ctccaacgtc ttcatgacct tcgcctggta
     9901 cggccacttg cgggggctgg aaaagcagcc gtggctggtg gcggcgctgg tgagctgggg
     9961 gatcgcgctg ctcgaatacc tgctgcaggt gccggcgaac cggatcgggg cgctagaggc
    10021 tcacctgcaa gtgggccagc tgaagatcat gcaggaagtg atcacgctca gcgtgttcgt
    10081 gccgttctcg atctggttcc tcaaggagcc gatcaagctc gactacctgt gggccgccct
    10141 ctgcttgtgc ggagcggtgt tcttcgtgtt ccgcggcgag ctgacggctg ccgccctggg
    10201 caagtaagca tgcacgccgg tgaagcgacg acgcggacct tcgctccgct tttggaaagg
    10261 ccggcggacg gcgaaaaatc cccgccgcgg ggcgcgccgc cgctcgtccg actgcggtgg
    10321 gtgatcgccg cgattctcgt tgggctcgcc gctttctcgg ctttgcggct gtggtttgtc
    10381 gagggactgc tgcggccggc gaccatcgac ggcccttcga tggcgccgct gctgctgccc
    10441 gcacattttg cggtcacttg cggcgactgc ggctttcgct ttgcctgcga cgccgagcgt
    10501 ccgccgcccg cgggccaggc ggtctgcccg aactgcggct acgcaggcaa cgatttggca
    10561 cgggccaaag cgcgtcccga
//
LOCUS       DASRJB010000779         7125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3892627, whole genome shotgun sequence.
ACCESSION   DASRJB010000779 DASRJB010000000
VERSION     DASRJB010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3892627"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFB80_20595"
     CDS             <1..614
                     /locus_tag="VFB80_20595"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36245.1"
                     /translation="TSIFNGKDLTGWRGDKTYWSVQDGALTAKTTPENRLTYNTFLIW
                     EGGQPGDFELRAKYKIVGGNSGIQYRSKVLDEAKFVVGGYQADIDSTPRYTGILYEER
                     FRAILAERGTKATVGADGKKEATTFADKDELQAKIKKEDWNDYTIIAKGNHLQHYING
                     TLMSETIDNQKDKAATTGILALQAHAGPPMTVQFKDLRIKELK"
     gene            700..1200
                     /locus_tag="VFB80_20600"
     CDS             700..1200
                     /locus_tag="VFB80_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36246.1"
                     /translation="MEPNPYESPRTGKPLSGVNVAKRSLALAILLLLTPPAMAIAVFG
                     SCKALYVFPSDLLGIAYVFPFLVLAGLMIAGAVLWWPRQDVPATKEHRVGLLLATPFA
                     VAAATAVGFGLAVVGYFCAGRPGGNMIPGGEMLVLLVFWLPPTGTLLYMLWLAWRHRF
                     RSQPQR"
     gene            1263..1454
                     /locus_tag="VFB80_20605"
     CDS             1263..1454
                     /locus_tag="VFB80_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6800 family protein"
                     /protein_id="HZN36247.1"
                     /translation="MGGIIERQKELRQRRHRKKKLKVFKRRVAKATQSEKAHIAAKIR
                     RLTPGAEEIIKTLHLEERK"
     gene            complement(1546..2322)
                     /locus_tag="VFB80_20610"
     CDS             complement(1546..2322)
                     /locus_tag="VFB80_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN36248.1"
                     /translation="MTVLVKKNPPSGTIVLSRPDKRNALSRQMLADLMQALDDLHQER
                     KVRAVILTGAGTAFCAGMDLAEMQATAQQPDSQSQWHRDAVQYRELIDKLLQYPKPII
                     AAVNGPALAGGAGLVLASDIVVGTPEARFGLPEPKRGIVAGLVSPLLVFRVGGSHAAN
                     LLLTARTIEASEAQRIGLYHEVIKNDLVWARAQEIAGEIAASAPEALLLTKRMLNETI
                     GEHLQTQLSAGAATSATARTTEAAREGLAAFLEKREPKWQ"
     gene            complement(2354..2698)
                     /locus_tag="VFB80_20615"
     CDS             complement(2354..2698)
                     /locus_tag="VFB80_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36249.1"
                     /translation="MTIGKTIRVRDLASARSGDKGNHANIGVVANDEAAYGVLVSRLT
                     AVRVQTHFQELGVSRVERFELPKVLALNFVLYDALGGGASRSLRIDTQGKLLATSLLD
                     LTFHANAINDDD"
     gene            2784..4202
                     /locus_tag="VFB80_20620"
     CDS             2784..4202
                     /locus_tag="VFB80_20620"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36250.1"
                     /translation="MTEPSIQPVVSRLPRRSFVGGVAAGAATVAAAATSATAASGKIG
                     VGFIGYGLIGKRHVLDFQAQPDVNIVAVSDCHAGRLDEAKALIGGNVRGYADFRELLD
                     DRDVEAVVISTPDHWHALMAMLACAAGKDVYVEKPVTLFPREGAWMLAVATKHRRVVQ
                     VGTQQRSGPHYAAARKLIQSGHIGKIVSVRMQSFRNIMPGFGQPPDSPPPAGFDWNMF
                     LGPAPDRPYNPNRGLYHFRWFWDYSGGQMTNLGQHSLDIVDWFLGPLVLKSVASFGGR
                     FALADNGETPDTQDAVFDFSGFTFPGFTVTWSHREASQGESTGPGLVFYGTKGSLSIS
                     RSGYAITPDRVIRPENAVPQFTDAQPVGGVARQSEPMETRLWTTAGSDRRGDSQGQFV
                     RHVRNFLDCVKTRHPPVSDLASGQRVATYCHLANLSLRLGRSLQWDARQETIRNDADA
                     ALLLDRPYRAPWDAVLKAVLAS"
     gene            complement(4223..5197)
                     /locus_tag="VFB80_20625"
     CDS             complement(4223..5197)
                     /locus_tag="VFB80_20625"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZN36251.1"
                     /translation="MNTPATASAAGPLLPIRRALLRGLAVILPPLLTVVVFLWAWNMI
                     DTYILVPCENIAGQLAVWAVRDVKSGVPDGAVVYREQPQGPVAKFDYKGTTYVPVGAS
                     NKYIPAEVFNNVVANPRQDSPAPVTANAYFDHYVHAKYLRRERTVPMFLATFLLVLYL
                     TGKFMAAGVGRYVRRIGEGVVGRMPIVRDVYSAAKQITETIFSEQELQFHRVVAVEYP
                     RKGLWTIAFVTGEGFKDIRDAAGEPILSLLVPTSPMPATGFTCTALKRDTIELDITVD
                     QAAQFIVSCGVVIPPHQQWNAKSREIRDDITRQVLDQADHAQRLVNSA"
     gene            complement(5607..6323)
                     /locus_tag="VFB80_20630"
     CDS             complement(5607..6323)
                     /locus_tag="VFB80_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZN36252.1"
                     /translation="MKLSLLITCLGDVIRPDAGKAVVRILRRLGHEIDFPQAQTCCGQ
                     PMFNSGYAELAREQAKHTIGVFTGDSAVIVPSGSCAAMVKVEFPHLLEGDKQWHPRAL
                     DLARRTFEFSDFLVNRLQVVDVGARFVGKVAFHFACHSRMLGLRNEVETLVKHVAGAT
                     YVPLNRQDQCCGFGGSFAIRYPQISGSMVEDKMQCILASGADCVVSTDAGCLMNIGGR
                     LHRAGKTVELLHIAELLDRR"
     gene            6419..7096
                     /locus_tag="VFB80_20635"
     CDS             6419..7096
                     /locus_tag="VFB80_20635"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN36253.1"
                     /translation="MSLAPSISEIEYPESDGKPMGETDLHRYWMNRLYDLLSRRYAGR
                     PAYVGCDLLVYFTEGRVFDVCVPDVFVALGAEPGFRRTFKTWVEGRPPTVVFEVTSQS
                     TRREDEVFKPRTYARIGVSEYFLYDPTAEYLTPPLMGYRLLGEGRGPIAADGSGAIRS
                     HELGVKFWLDDKGLVAADSATGQLLLTDAEAAEAQADAARARAAAAELEIQRLKAELA
                     RLKPPTG"
BASE COUNT         1261 a         2326 c         2282 g         1256 t
ORIGIN      
        1 ttaccagcat cttcaacggc aaggacctca cgggctggcg gggcgacaag acctactgga
       61 gcgtgcagga cggggcgctg accgccaaaa cgacgcccga gaatcgcctg acttacaaca
      121 cctttctgat ctgggagggc gggcagcccg gcgatttcga gctgcgagcc aagtacaaga
      181 tcgtgggcgg caactccggc attcaatacc gcagtaaggt tctcgacgaa gccaagttcg
      241 tcgtcggcgg ctaccaggcg gacatcgatt ccacgccgcg ctataccggc atcctgtacg
      301 aagagaggtt ccgcgccatc ctggccgagc ggggcaccaa ggccacggtg ggcgccgacg
      361 gcaagaagga agcgaccacg ttcgccgaca aagatgagct gcaagccaag atcaagaagg
      421 aagactggaa cgactacacg atcatcgcca agggcaacca cctgcagcac tacatcaacg
      481 gcacgctgat gtcggaaacg atcgacaacc agaaggacaa agccgccacc acgggcatcc
      541 tcgccctgca ggcccacgcc ggcccgccga tgaccgtgca gttcaaggac ctgcggatca
      601 aagagctgaa gtagggcgct cgcgatctgc aatggctgaa tcaaccgcgg ccccgccacc
      661 gtctggcggg gctttttcat gcgcggctag actgcccaga tggagcccaa cccgtacgaa
      721 tcgccgagaa ccggcaaacc gctgagcgga gtcaacgttg ccaagcgcag cctcgcgctg
      781 gcgattctcc tgctgctcac gccgccggcg atggcgatcg ccgtgtttgg ctcctgcaag
      841 gccctttacg ttttcccgtc ggatctgctt gggatcgcgt atgtatttcc atttctcgtg
      901 ctcgccgggc tgatgatcgc tggggcggtg ctctggtggc cgcgccaaga cgtccccgcg
      961 accaaagaac accgcgtcgg cctgctgtta gcgacgccct tcgcggtagc cgcggcaacc
     1021 gccgtggggt tcgggctggc ggtggtgggc tatttctgtg ccggcaggcc aggcgggaac
     1081 atgatccccg gcggcgagat gctggtgctg ctcgtctttt ggctgccgcc taccggcaca
     1141 ttgctgtaca tgttatggct ggcgtggcgg catcgctttc gctcacagcc gcagcgttga
     1201 cgggccgcca gcgcactgat actttggtgg attcccctga tcctggcggc ggagcaatcg
     1261 gcatgggcgg cattatcgaa cggcaaaagg agctgcggca gcggcggcat cggaagaaga
     1321 agctcaaggt cttcaaacgg cgcgtggcca aagcgactca gagcgagaag gcccacattg
     1381 cggccaagat tcgccgcctc acgccgggcg ccgaggagat catcaagact ctccatttgg
     1441 aagagcggaa gtaacgcgcc gaaattccaa gaaccaaaga tccgaagaac caaattccgc
     1501 ccttttggtt ctttggatct tcggttcttg ggttttccta ttctcctact gccacttggg
     1561 ctcgcgcttc tccagaaatg ccgcgagccc ttcgcgggcc gcttcggtcg tgcgggctgt
     1621 ggcgctagtt gccgcaccgg cagacagctg cgtctgcagg tgctcgccga tcgtctcgtt
     1681 cagcatccgc ttcgtgagca atagcgcctc gggggcgctg gcggcgattt ctcccgcgat
     1741 ctcctgggcc cgcgcccaaa ccaggtcgtt ctttattact tcgtgataga ggccgatccg
     1801 ctgcgcttcg gacgcctcga tcgtgcgtgc cgtgagcagc aggttggccg cgtgcgagcc
     1861 cccgacgcgg aagaccaaga gcggcgagac cagcccggcg acgatcccgc gcttgggctc
     1921 gggcagacca aagcgggcct cgggcgtgcc gacaacgata tcgctggcaa gcaccagccc
     1981 cgcgcctccc gccagcgccg ggccgttgac cgccgcgatg atcggcttgg gatactggag
     2041 cagcttgtcg atcagctccc ggtactgcac cgcatcgcga tgccattgcg actgggagtc
     2101 gggctgctgc gcggtcgcct gcatctcggc caggtccatc cccgcgcaaa acgccgtccc
     2161 cgccccggtc agaatcaccg cccgcacctt ccgctcctgg tgcaggtcgt ccagcgcctg
     2221 catcaggtcc gcgagcatct gccgcgagag cgcgttccgc ttgtccggcc ggctcaagac
     2281 gatcgtgccg gagggcgggt tctttttgac aaggacggtc attggccgcg agccagggat
     2341 caggagtcgg gagtcagtcg tcgtcgttga ttgcgttggc gtgaaatgtc agatcgagca
     2401 gcgacgtggc cagcagcttc ccctgcgtgt cgatccgcag gctgcggcta gcgccgcccc
     2461 cgagcgcatc gtagagcacg aagttcagcg ccagcacttt cggcagctcg aaccgctcga
     2521 cgcgcgagac gcccagctcc tgaaaatgcg tttgcacgcg aaccgccgtc agccggctga
     2581 cgagcacgcc gtaggccgcc tcatcgttgg ccacaacgcc gatgttggcg tgattgccct
     2641 tgtcgccgct gcgggcgctc gccaggtcgc gaacgcgaat cgttttgccg atggtcatgg
     2701 ccgcttcccg ccgcgaatgc cgtccgccgc tgattttact ggtcggccgc gattggatat
     2761 agtccggact taggagccct ggcatgaccg agccatccat ccaacctgtt gtttcgcggc
     2821 tgccccggcg gagttttgtg ggcggagttg cggcgggcgc tgcgacggtc gccgccgcgg
     2881 caacttcggc aaccgcggct agcggcaaaa tcggcgtcgg cttcatcggg tacgggctga
     2941 tcggcaagcg gcacgtgctc gactttcagg cgcagcccga cgtaaacatc gtggccgtgt
     3001 cggactgcca cgccggccgg ctggacgagg ccaaggcact gatcggcgga aatgtccgcg
     3061 gctacgccga cttccgcgag ctgctggacg accgcgacgt cgaggcggtc gtgatttcga
     3121 cgcccgatca ctggcacgcc ctgatggcga tgctggcctg cgccgccggc aaggacgtgt
     3181 atgtcgaaaa accggtaacg ctctttccgc gggagggagc gtggatgctc gcggtcgcga
     3241 ccaagcaccg ccgcgtggtg caggtcggta cgcagcagcg ctctggcccg cactacgccg
     3301 ccgcccgcaa gctcatccaa agcggccata tcggaaagat cgtcagcgtg cggatgcagt
     3361 cgttccgcaa catcatgccg ggcttcggcc agccgccgga cagcccgccg cccgcgggat
     3421 tcgactggaa catgtttctc ggcccggcgc ccgaccggcc gtacaacccc aaccgcggcc
     3481 tgtaccactt tcgctggttc tgggactact ccggcgggca aatgacgaac ctcggccagc
     3541 actcgctcga catcgtcgat tggttcctcg gtccgctggt gctgaaatcc gttgccagct
     3601 tcggcggccg gttcgccctg gccgacaacg gcgagacccc cgacacgcag gacgccgtct
     3661 tcgatttttc gggcttcact tttcctggtt tcactgtgac ctggtcgcac cgcgaggcat
     3721 cgcagggcga atcaacaggc ccgggactcg tgttctatgg cacgaaaggc tcgctctcga
     3781 tctcccgcag cggctacgcc atcacgcccg accgtgtgat ccgcccggaa aacgccgtgc
     3841 cgcagttcac cgacgcccag ccggtgggcg gcgtcgcgcg acagtccgag ccaatggaaa
     3901 ccaggctctg gacgacggcc ggcagcgacc gccgcggcga ttcgcaaggc cagttcgtcc
     3961 gccacgtgcg gaatttcctc gactgcgtca agacgcggca cccgcccgtt tccgacctgg
     4021 cgagcggcca gcgcgtggcg acctattgcc acctggcgaa cctctcgctc cgcctcggcc
     4081 gctcgctgca gtgggacgcg cggcaagaga cgatccgcaa cgacgccgac gccgccctgc
     4141 tattggaccg tccctaccgc gcgccgtggg atgcggtgct gaaggccgtg ctcgcctcgt
     4201 agggcaagct gccagcttgc ccttaggccg aattgaccag ccgctgggcg tggtcggcct
     4261 ggtcgagcac ttgccgggtg atgtcgtcgc ggatttcgcg gctcttggcg ttccactgct
     4321 ggtgcggggg aatgacgacg ccgcagctca cgatgaactg cgcggcctga tcgacggtga
     4381 tgtcgagctc gatcgtgtcg cgcttgagcg ccgtgcaggt gaaacccgtg gcgggcatcg
     4441 gcgaggtggg cacgagcagg ctgaggatcg gctcgccggc ggcgtcgcgg atgtccttga
     4501 agccttcgcc ggtgacaaac gcgatcgtcc acaggccctt gcggggatac tcgacggcga
     4561 ccacgcgatg gaactgcagc tcctgctcgc tgaagatggt ttcggtgatt tgcttggcgg
     4621 ccgaatagac gtcgcggacg atcggcatgc ggccgacgac gccttcgccg atgcggcgga
     4681 catagcggcc cacgcccgcg gccatgaact tgcccgtcag gtacagcacc agcaggaagg
     4741 tcgccaggaa catcggcacc gtccgttcgc ggcggaggta tttcgcgtga acatagtggt
     4801 cgaagtaggc gttggccgtg accggggcgg gagaatcctg ccgcgggttg gccacgacgt
     4861 tgttgaacac ctccgccgga atgtacttgt tgctggcacc gaccggtacg tacgtcgtgc
     4921 ctttgtagtc gaatttcgcg accggaccct gcggctgctc tcgatagacc actgccccat
     4981 cgggcacacc cgacttcacg tcgcggaccg cccagacggc cagttgcccc gcgatgttct
     5041 cgcagggcac gaggatatag gtgtcgatca tgttccaggc ccacaggaac acgaccaccg
     5101 tcagaagcgg cggcaggatg acggccagcc cgcgcaggag cgcccggcga atcgggagaa
     5161 gcggaccggc ggcagatgcg gtggcgggcg tgttcatggc tccccttctg gcagacgttg
     5221 gcgcgggcgt tttcagattc taggtctgcg gaagcgttcc ggcgggcgag gtccagcgcg
     5281 atggcgacgg tcgtagtgtc gcctgtcagg ccgctacagc cgttgccttc ggcgcgcaac
     5341 gagtaatcgg ccgccaacac gcggccgggg agcggaagac cgacctgtga aacgcgcttc
     5401 ggccccaata ttgacctata tcttgagttg accgaagtct cttccaccac tcacagttcg
     5461 gtagcgccat gcccgcccgc agctcaattc gtggacgcat ctcgcgctgg atgattgtct
     5521 gcacgtttgt ggtgtgcatc gccaagctgt cgagcggcta cgttcctccc gcccccacgc
     5581 cttcccgcgt gccagctgcc aaggcgctac cgccggtcga gcaattcggc gatatgcaat
     5641 agctccaccg tcttccccgc gcggtgcagc cggccgccga tattcatcag gcagcccgca
     5701 tcggtcgaga caacgcagtc ggccccgctg gccaggatgc actgcatctt gtcttccacc
     5761 atgctgcccg agatctgcgg atagcggatt gcaaaagagc cgccgaagcc gcagcactgg
     5821 tcctgccggt tgagtgggac gtatgtcgcg ccggcgacgt gcttcaccag cgtctcgacc
     5881 tcattcctca ggccgagcat tcgcgagtgg caggcgaagt ggaaggcgac tttgcccacg
     5941 aaccgcgcgc cgacatcgac gacttgtaag cgattgacca ggaagtcgct gaactcgaac
     6001 gtccgccgcg cgaggtccag cgctcgcgga tgccattgtt tgtcgccctc cagcaagtgc
     6061 ggaaactcca ctttgaccat cgccgcgcac gagccgctgg gcacgatcac ggcggaatcg
     6121 cccgtgaaca caccgatggt gtgcttcgcc tgctcccggg ccaattccgc atagccgctg
     6181 ttaaacatcg gctgcccgca gcaggtctgt gcctgcggaa agtcgatctc atgccccagc
     6241 cgccgcagaa tccgcaccac cgccttgccg gcatcgggcc ggatgacgtc gccgaggcag
     6301 gtgatgagga gggagagctt catcgtgcgc gtccgatcga gcaagaattg ctgtcgcggt
     6361 ggcgtatggc tggtacaata cacgagatgt tcgcgagccg cgcggctagg aaacagaaat
     6421 gagtctggcg ccctcgattt ccgaaatcga atatcctgaa tcggacggca agccgatggg
     6481 cgaaaccgat ctgcatcggt attggatgaa tcgcctgtac gacctgctca gccggcgata
     6541 cgctggccgg ccagcctacg tcggctgcga cttgctcgtg tatttcacgg aaggccgagt
     6601 attcgacgtg tgcgtgcccg acgtcttcgt ggcgctcggc gccgagcccg gctttcgccg
     6661 cacattcaaa acctgggtcg agggtcgccc gcccaccgta gtcttcgaag tgacttcgca
     6721 gagcactcgc cgagaagacg aggtctttaa gccgcggact tacgcgcgaa tcggcgtgag
     6781 cgagtacttt ctctacgatc cgacggcgga gtaccttacg ccgccactga tgggatatcg
     6841 gctactcggc gaaggccgcg ggccaatcgc ggccgacggc agcggggcga tccgctcgca
     6901 cgagttgggt gtaaaattct ggctcgacga caagggactt gtcgccgccg actccgccac
     6961 gggacagctg ctattgaccg atgccgaagc ggctgaagct caagccgacg cagccagggc
     7021 tcgcgcggcc gcggcggaac tggaaatcca gcgattgaaa gcagagttgg ccaggctcaa
     7081 gccgccaacc ggctaaaccc ggtccggcag atttccttcg gcgtc
//
LOCUS       DASRJB010000780         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2194840, whole genome shotgun sequence.
ACCESSION   DASRJB010000780 DASRJB010000000
VERSION     DASRJB010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194840"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFB80_20640"
     CDS             complement(<1..192)
                     /locus_tag="VFB80_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36254.1"
                     /translation="MRACPPLPNDHCTAIRRGGSTQKSRWSFSLLTLLLATGLIMAVI
                     SHVRTSRILENYREKSDDLE"
     gene            complement(289..1692)
                     /locus_tag="VFB80_20645"
     CDS             complement(289..1692)
                     /locus_tag="VFB80_20645"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36255.1"
                     /translation="MTDFQLNRRQALIVGGLGALSMGMPGAVIGSDQLDAQGNASRAE
                     KSCIFVLLCGGPSHVDTWDMKPNAPASIRGPYQPIATKVPGMRINEMHTQLAQRTDDF
                     LLINSMTHPGSISNHFDAMHNLLSGQSVVRVREGMPDDQPYLGSFVARHKPSKRNLVS
                     NAWLIKCVGPPVFCAPNIGIGGYLGSAYAPVFVGSAENHPAMATFKPPEIYDFGNEED
                     RNRRKDLLSSLESRKLDRDARGKDWSDLRAKAYEALTRPEGRQAFDMSREPDKVRERY
                     GMHPLGQNLLLARRMVEAGVRFVTVNGWAGQSTHDKQGPPSSSWDMHGGNMGMGNAFG
                     DGSYGMGFCLPRLDQALSALLTDLKDRGLLKNTLVVCMGEFGRTPNILTQEPPGRQHW
                     PQCFSAILAGCGIRGGAVYGKSDKHGAQVESNPVRPEELAATIYHALGVPLNAPQNNS
                     GISRPITTGKPIMELFG"
     gene            1884..>2121
                     /locus_tag="VFB80_20650"
     CDS             1884..>2121
                     /locus_tag="VFB80_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36256.1"
                     /translation="MLRWKTILFAGLLTSGLIGAAAADDRQQWQGTWTMVSCVANGES
                     TEGNVQWVVSGDQYKIRLDGKLGSDPYPFQLDPSK"
BASE COUNT          358 a          624 c          758 g          381 t
ORIGIN      
        1 ttccaggtcg tccgactttt cgcggtagtt ttcgaggatc cgcgacgtgc ggacgtggga
       61 aatgaccgcc atgatcaggc ccgtcgccaa cagcagcgtg agcagcgaga acgaccaccg
      121 cgatttttgg gtggagccgc cgcggcggat tgcggtgcag tggtcattgg ggagcggggg
      181 gcaagcacgc acctcggccg tcaagagcaa gacgaacgag gagggcggct ggccttcgat
      241 ccacgcggcg ggagcaagag aaagagcaat cgtccgcccg gctgcgattc acccaaacag
      301 ctccatgatc ggcttgccgg tggtgatggg gcggctgatg ccgctgttgt tttgcggggc
      361 gttgaggggg acgccgaggg cgtggtagat ggtggcggcg agctcttcgg ggcggacggg
      421 attgctttcg acttgggcgc cgtgcttgtc ggacttgcca tagacagcgc cgcctcggat
      481 gccgcagccg gcgaggatcg cggagaaaca ctggggccag tgctgccggc cgggcggctc
      541 ctgggtcagg atattgggcg tgcggccgaa ctcgcccatg cagacgacga gcgtgttttt
      601 gaggaggccg cggtccttca ggtcggtcag cagggcggag agggcctggt ccagccgcgg
      661 caggcagaag cccatgccgt aggagccgtc gccgaaggcg ttgcccatgc ccatgttgcc
      721 cccgtgcatg tcccaactgc tgctgggcgg gccctgcttg tcgtgcgtcg attggccggc
      781 ccagccgttg acggtgacga accgcacgcc ggcctcgacc atccgccggg ccagcagcag
      841 attctgcccc agcgggtgca tgccgtaacg ctcgcggacc ttgtccggct cgcggctcat
      901 gtcgaaggct tgccggcctt cggggcgggt cagggcttcg taggccttgg cccgcaggtc
      961 cgaccagtct ttgccgcggg cgtcgcggtc gagcttgcgg gactcgaggg acgaaagcaa
     1021 atctttgcgg cggttgcggt cctcctcgtt gccgaagtcg tagatctccg gcggcttgaa
     1081 ggtggccatc gccgggtggt tttcagccga gcccacgaac acgggggcgt aggccgagcc
     1141 gaggtagccg ccgatgccga tgttcggagc acaaaagacg ggcgggccga cgcacttgat
     1201 cagccaggcg ttggagacga ggttccgctt gctgggcttg tgccgggcca caaacgagcc
     1261 gaggtacggc tgatcgtcgg gcatgccctc gcggacgcgg acgaccgact ggccgctgag
     1321 caggttgtgc atcgcgtcga agtgattgct gatcgagccc gggtgggtca tcgaattgat
     1381 caacagaaaa tcgtcggtcc gctgcgcgag ctgcgtgtgc atctcgttga tccgcatccc
     1441 cggcaccttg gtggcgatgg gctgatacgg cccgcggatc gaggccggcg cgttgggttt
     1501 catgtcccag gtatcgacgt ggctcggtcc gccgcagagg agcacgaaaa tgcacgactt
     1561 ctccgcccgg gacgcgttgc cctgcgcgtc gagctgatcg ctgccgatca ccgcccccgg
     1621 catccccatg ctgagcgcgc ccaggccgcc cacgatgagc gcctgccgcc ggttcagttg
     1681 gaagtcagtc atgctgcaac tccgatgcgt tctggaatgc cccgattctg agcaatcgag
     1741 cgatcgttgt caaagacaat ggcaggtcgt cctcggcgca atcggcataa agcggcgcgg
     1801 caagcattaa ttccgccagc agcggcaggg ctagggcgta gatagtagca accgtgtttc
     1861 caatcacatt tggaggaatt gccgtgcttc gctggaagac cattctgttt gccggtttgc
     1921 tgaccagtgg gctgatcggg gcggcagcgg ccgacgacag gcagcagtgg caggggacct
     1981 ggacgatggt gtcgtgcgtc gctaacggcg aatcgacgga gggaaacgtg cagtgggtgg
     2041 tgagcggcga ccaatacaag attcgccttg atgggaagct gggctcggac ccttatccct
     2101 tccagctcga cccgagcaag a
//
LOCUS       DASRJB010000781         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1917341, whole genome shotgun sequence.
ACCESSION   DASRJB010000781 DASRJB010000000
VERSION     DASRJB010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917341"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1041
                     /locus_tag="VFB80_20655"
     CDS             37..1041
                     /locus_tag="VFB80_20655"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZN36257.1"
                     /translation="MRGGSCTCELSAAWRAACCTLLLAGGCRPNHAGSTAPPPTVAAR
                     QPQIFIADWANHRVVRVTGLHGEDWTTCGESGPVDLRYPVGICLDSSGRLYVAEQEPR
                     LHRLDQLYGAGWVTHQLEPEPNRPPNRHLGCWVCLDRSGRIYVSDGGRHRIVRIDDMS
                     GAGRVSLGSAGSGERQFHHPAGVWVDSQDRLYVADFDNFRIVRCNDMQGTNWTELGRY
                     GSGEREFINPAGICIDSQGRIYVADQGNDRVVRIDDMRGANWTTVGSFGIGDAPQRLY
                     APTGIAVDRAGRIYVTQCSSNHRLVRMDDMSGANWTVFGGGGVGSGQFASPLGVFVRE
                     "
     gene            1051..2100
                     /locus_tag="VFB80_20660"
     CDS             1051..2100
                     /locus_tag="VFB80_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36258.1"
                     /translation="MTPIALAGLACWLCGQAAAQTPQIEVLRAAVGKSLPLLQAGAQS
                     FRERSEGRCISCHHQGLILTTVALARSRGLAVNENLARAEIERVRGFYARRQQRYQAA
                     LEDSAALPQADPFGNFTVHAGYWLWGLAAENVAPDEALATTARLLAGRQQADGRWTFT
                     DTARAPMQAGGITTTALAAFAINRYGPAADRESRDERLRRARQWLLEEPPQTTDDLAF
                     GLLGLKWTGAPDGDLQAAAARLRAAQRSGGGWAQQANMPPDAYATGLALFALHEGGTA
                     VTDPAWRQGLAHLLQSQQPDGSWFVRTRAIPSNPYFESGFPHGKSQFISYAATCWATV
                     ALTLALPVGSPVGRE"
     gene            2162..3370
                     /locus_tag="VFB80_20665"
     CDS             2162..3370
                     /locus_tag="VFB80_20665"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN36259.1"
                     /translation="MNRSLRSQALSRRGLLGLGCAAGAALLAEQDSSAQNPAAAAQDR
                     TSSIRLTSLRATICRDRVFVRLDTNHGVSGWGEIKGVVPTVAAALAEAMFKLLDGQNP
                     TRIEHLWQTLYRAERNQRGGAFMLHTIAGIDMALWDITGKLWGVPVYRLLGGPTRDKI
                     RVYPSTKAVKIGNQPKPQSADPAVIEQMVAAVRAARQRVGKDGSVMFDAHSAVPPATL
                     IQFASAIEPYEILFLEEPAVPGNIETFKRIKQQVRIPLACGERDRTIWGILPYLAENA
                     IDIVQPDCGYTGGITQMKKIATLAEAYYVPLAPHCTQSYLGMTASFHVAAAVPLFLIH
                     EAYDDELWGKIVRRNWTKSDDGQVTLPQGTGLCLDIDETALAAAAKDPSYQYQWRGPK
                     LDPDGSIADY"
     gene            3460..>3699
                     /locus_tag="VFB80_20670"
     CDS             3460..>3699
                     /locus_tag="VFB80_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36260.1"
                     /translation="MPYDEIYLNSIMRIMRAASSESLRLRDVHAYRRKSRTAYHDVTA
                     AATSTYAVNPAFALCRICTLRDPAVHILNIPASAAA"
BASE COUNT          624 a         1278 c         1201 g          596 t
ORIGIN      
        1 cggacaaggc cgactttgcc ttgaaatcga attgagatgc gcgggggaag ctgcacctgc
       61 gaactctcgg ccgcctggcg ggccgcctgc tgcacgctgc tgctggctgg cggatgccgt
      121 ccgaaccacg ccggttcgac agcgcctccg ccgactgtcg ctgctcgcca gccgcagatc
      181 ttcatcgccg attgggccaa ccatcgcgtg gtgcgagtga cgggcctgca tggcgaggac
      241 tggacgacct gcggggagtc cggcccggtg gacttgcggt atccggtcgg catttgcctc
      301 gattccagcg gccgtctgta tgtcgcggag caagagccgc gactgcaccg cctggaccag
      361 ctctacggtg cgggctgggt cactcaccag ctggagccgg agccgaatcg cccgcccaac
      421 cgccacttgg gctgctgggt ctgcctcgat cggtccggtc ggatttacgt cagcgacgga
      481 gggcggcacc gcatcgtccg gatcgacgat atgagcggcg ccgggcgcgt cagcctcggc
      541 tcggccggca gcggcgagcg gcagtttcac cacccggccg gcgtctgggt ggatagccag
      601 gaccggctgt acgttgccga cttcgacaac tttcgcatcg tgcgctgcaa cgacatgcaa
      661 ggaacgaact ggaccgagct cggacgctac ggcagcggcg agcgggagtt catcaacccc
      721 gcggggattt gcatcgatag ccaggggcgg atttacgtcg ccgaccaggg aaacgaccgc
      781 gtcgtccgca tcgacgacat gcgcggcgcc aactggacga cggtgggctc gttcggcatc
      841 ggcgacgcac cgcagcgtct gtacgccccg acgggaatcg ccgtcgatcg cgcgggccgc
      901 atctacgtca cccagtgcag cagcaatcat cgcctggtgc ggatggacga catgagcggc
      961 gcgaattgga cggtctttgg gggcgggggc gtcgggtcgg gccaatttgc ttcgccgctg
     1021 ggtgtattcg ttcgcgaatg aggcttccct atgacaccga tcgcgctcgc cggactggcc
     1081 tgctggctgt gcggccaggc tgccgcgcaa acgccgcaga tcgaggtcct gcgcgcagcc
     1141 gtgggcaaga gcctgccgct gctgcaggcc ggcgcgcagt cgtttcgcga acgctccgag
     1201 gggcggtgca tctcctgtca ccatcagggg ttgatcctga cgacagtcgc cctcgctcgc
     1261 agccgcggcc tggccgtgaa cgaaaacctg gcccgcgccg agatcgagcg cgtccgcggg
     1321 ttctatgctc gccggcaaca gcgctatcag gcagccctgg aggactctgc ggccctgccg
     1381 caggccgatc cgtttgggaa tttcaccgtg catgccggct actggctgtg gggactcgcc
     1441 gccgaaaacg ttgcccccga tgaggcgctc gcgacaaccg cccggctgct ggcgggccgg
     1501 caacaggccg acgggcggtg gaccttcacc gatacggccc gcgcgccaat gcaggccggc
     1561 ggcatcacca cgaccgcgct ggcggccttc gcaatcaatc gctatggccc agcggccgat
     1621 cgggaatcgc gcgacgagcg gctccgccgc gcccggcagt ggctcctgga ggagccgccg
     1681 cagaccaccg acgacctggc gtttggcctg ctcggcttga agtggaccgg ggcgcccgac
     1741 ggcgacttgc aagcggcggc cgcacgcctg cgagccgcgc agcggagcgg cggcggatgg
     1801 gcccagcaag ccaacatgcc gcccgacgcc tatgccacag gcctggcgtt gttcgcgctc
     1861 cacgaaggcg gcacggccgt caccgatccc gcctggcggc agggactggc gcatctgctt
     1921 caatcgcagc agcccgacgg ctcgtggttc gtaaggacgc gggcgattcc cagcaatccc
     1981 tacttcgaaa gcggcttccc gcacggcaag tcgcagttca tctcctatgc ggccacctgc
     2041 tgggccacgg tcgccctgac gcttgcgctc cccgtcggct cgcccgttgg acgcgagtga
     2101 cgacggccct cctttcgctt cgagtgaaac accgcttcct cctctatcgc tggagttgtt
     2161 catgaaccga tctttgcgtt cgcaagccct ttcgcgccgc ggactgttgg gactgggctg
     2221 cgccgcgggc gcggcgctgc tggccgagca ggactcgtcc gcccagaatc ccgcggccgc
     2281 cgcccaggac cgcacgtcgt cgattcgact caccagcctg cgggccacga tctgccgcga
     2341 ccgcgtgttc gtcaggctcg acaccaacca cggcgttagc ggctggggcg agatcaaggg
     2401 cgtggtgccc acggtcgcgg cggccctggc cgaggcgatg ttcaagctgc tcgacgggca
     2461 aaacccgacg cggatcgagc acctctggca gacgctgtac cgcgcggagc ggaaccagcg
     2521 cggcggcgcg ttcatgctgc acacgatcgc cggcatcgac atggccctct gggacatcac
     2581 cggaaaactc tggggcgtgc ccgtctatcg gctgctgggc gggccgaccc gcgacaagat
     2641 ccgcgtctat ccctcgacca aggcggtgaa gatcggcaat cagcccaagc cgcagtcggc
     2701 cgatccggcc gtgatcgagc agatggtcgc ggccgtccgc gcggcccggc agcgcgtcgg
     2761 caaggacggc tcggtcatgt ttgacgccca cagtgcggtg ccgccagcca cgctgattca
     2821 gttcgcctcg gcgatcgagc cctacgaaat cctgtttctc gaagagcccg cggtgcccgg
     2881 aaacatcgag accttcaaac gcatcaagca gcaggtccgc attccgctgg cctgcggcga
     2941 acgcgaccgc acgatctggg gcatcctgcc gtacctggcc gagaacgcca tcgacatcgt
     3001 gcagccggac tgcggctaca ccggcggcat cacgcagatg aagaagatcg cgaccctggc
     3061 cgaggcgtac tacgtgccgc tggctccgca ctgcacgcag tcgtacctgg gaatgaccgc
     3121 cagcttccac gtcgcggcag ctgtgccgct gtttctcatc cacgaagcct acgacgacga
     3181 gttgtggggc aaaatcgtcc gccgcaactg gaccaagagc gacgacggcc aggtcacgct
     3241 gccccagggt accggactct gcctcgacat cgacgagacc gccctggccg ctgccgccaa
     3301 ggaccccagc tatcaatacc agtggcgcgg ccccaagctc gaccccgacg ggtccatcgc
     3361 ggactattga tgcggccaag gtcccacgac tgcgcctatg cgcgcatcat ttgaggtttt
     3421 tcgaaacggc agattcacgc cgcaactcca attgcggcaa tgccttacga cgaaatctac
     3481 ctgaattcca ttatgcgcat tatgcgcgcc gcctcgtccg agtcgctgcg gctccgcgac
     3541 gtgcatgcat atcgcagaaa atcccggact gcatatcacg acgtaactgc cgcagccaca
     3601 agcacttacg cggtgaatcc cgcgtttgca ttatgcagaa tatgcacgtt aagggaccca
     3661 gccgtgcata tcctcaacat tcccgccagc gcggctgcc
//
LOCUS       DASRJB010000782         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3603540, whole genome shotgun sequence.
ACCESSION   DASRJB010000782 DASRJB010000000
VERSION     DASRJB010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603540"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFB80_20675"
     CDS             <1..479
                     /locus_tag="VFB80_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36261.1"
                     /translation="ILPVLLSLLVLPAVTLFEPQQYHEPAIYEQTMPAEKSGAMFTPL
                     LPAAGSGYPALGLATLPLPIILGQQAAPPSVDRMGTVALLMPVLALFYIMNIALSITL
                     IVAGWRMRQLKSHGLSLVAAIMAMLPCTFGWIVGLPMGIWAFSVLQSAEVRAAFET"
     gene            complement(583..1530)
                     /locus_tag="VFB80_20680"
     CDS             complement(583..1530)
                     /locus_tag="VFB80_20680"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN36262.1"
                     /translation="MANSTPRHAYKEIQLQQLRSFCETARLGSLTAAARSLGLSQPTV
                     WEQVHSLQRDLGVSLIERHARGCRLTEEGRRVVTLAAPLVQGVRAFKRNVAEALEQET
                     SRLTIATTQRILVEDLPRPIVEFEKAHQQVQLRLLELRIEQVPQAVESGQADLGFTTE
                     QGTDRRNPWLGFERGYDLEVFLVTRTDHPLARRRQVAPRDLLKYPLLNSPQGIPDPTL
                     AVTLQKLGVFDVQPRRVEAFYTAVIRRYVELGFGIGLIVGLPGHPPTPGLHERSMSRY
                     FGQVTVNLVWRKGDPRTGAGGAFAQTVRSSLRPRRKRRR"
     gene            1718..3391
                     /locus_tag="VFB80_20685"
     CDS             1718..3391
                     /locus_tag="VFB80_20685"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN36263.1"
                     /translation="MAFRCLACFVGLVLFFPLAARAEYPFVFRDVGASAGVLPAAAGI
                     RGHGAGWGDVDGDGWLDLYVATFHNDGSQANLFFRNQNGKFEQDSQPSLKISARGTGV
                     LFADLDNDGDLDLYLGSMPSPAGSRLATKEGHPMLGNQLLRNDGGGKFTNISDANGAC
                     PAAFGGRSVTVLDFDGDGLLDILCGEEPVSGYNGSPTKSSRLFRNLGRLQFEDVSQQV
                     GLTAGVPGLGVAAADLTGDGWPDFFLASSGGGNVMFINDGRGKFREAPREVFAWPTAK
                     GDNMVCGVTFCDVNRDALPDMVLGQHFSNPWQQPVANRLYLHQGVKDGLPVFADVTEK
                     VGLVPLPLKAPHVELQDLDNDGWPDLYSSQVKFAGGQPYPLIFKNLGVKGGLPQFREE
                     MLAVNDFPTAEDRAIGRSGTLFDKVLKEKKIIYTAPGPTADFDNDGRLDMFLANWWIE
                     APSLLLKNETPGGNWLDLQVVGPPGVNRMGIGTRLLIYPAGKLGDAASLLGCREIAAG
                     YGYASGQPAIAHFGLGELASVDIEAILPHGKGKLTRKDVKANQRLTITQ"
     gene            3539..4843
                     /locus_tag="VFB80_20690"
     CDS             3539..4843
                     /locus_tag="VFB80_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36264.1"
                     /translation="MIACAFAQTPDERKAKKKLDRYPLPYPPTLAGGQTQVTENSPAF
                     LVPGPNLREGVLVAKTPPVVDFAFYPQQNYPGNPWSHRSDGIVRGDKYYSSSNDHLAP
                     RGTALLWEYDAVTKQFRLLCDTTKFLESQHAFPPGMNYRPGEMQSRIDLGSDGWLYYC
                     TDRGSPTVTDDAHGYLGEWILRTHPQTLATEIVATHPIEKHTLPASVLDPERMILYCG
                     TAVGKDAPNQKIQFFAFDLKSRKLLKVADDGPTRTLIFAKSTGCVFWEGKRYDPATNE
                     ITPASVPHVRSATAETPQGIVYGTSGTAADLWAFHVKTGELKQLGSAAVASQEYIASI
                     DADPTGRYLYYAPGAHGGAARDGTPVVQYDVRTGRKKVLAFLHQLFLDKHGYTPDGCF
                     GSALDEKGERLFISWDGWRKGQPRGWESCSLTVIHIPASERP"
BASE COUNT          827 a         1669 c         1556 g          843 t
ORIGIN      
        1 gcatcttgcc ggtgctactg tcgctgctgg ttcttcctgc ggtgacgctg ttcgaacccc
       61 agcaatacca cgagccggcg atttacgagc agacgatgcc agcggagaaa agcggcgcga
      121 tgttcacgcc gctgctgcca gccgctggca gcggttatcc ggcgctgggg cttgccacgc
      181 tgccgctgcc gatcatcctt ggccagcagg ccgccccacc ttctgtcgat aggatgggca
      241 cggtcgcgtt gctcatgccc gtgctggcgc tgttctacat tatgaacatc gccctctcga
      301 tcacgctgat cgtcgccggg tggcgaatgc ggcagctcaa gtctcacggc ttgtcgctgg
      361 tcgcggcgat catggcgatg ctgccctgca cgttcggctg gatcgtcggc ctgccgatgg
      421 gcatctgggc cttctcggtc ctgcaaagcg cggaggtccg cgcggcgttc gaaacttgag
      481 ctaagtgggg ccgcattgaa gcctgaagcg cgagcgaagg aattctcaga ccttcgctcg
      541 cgcttcaggc ttcaatgtgt ccacgacatt ccgcgggtcc acttaccggc gtcgcttccg
      601 gcgcgggcgc agcgaggagc gcaccgtctg ggcaaacgcc ccgccagcgc cggtccgcgg
      661 atcgcccttc cgccagacga gattcaccgt cacctggccg aagtaccggc tcatcgaacg
      721 ctcgtgcagc cccggcgttg gcgggtggcc cggcaacccg acgatcaggc cgatcccaaa
      781 ccccagctcg acatagcggc ggatcaccgc cgtgtaaaac gcctcgaccc gccgcggctg
      841 cacgtcgaac acacccagct tttgcagcgt cacggccagc gtcggatcgg gaatcccctg
      901 cggcgaattg agcagcggat acttgagcag gtctctcgga gcgacctgcc ggcggcgggc
      961 cagcggatga tcggtccgcg tgaccaggaa cacctccagg tcgtagcccc gctcgaaccc
     1021 cagccaggga ttgcggcggt cggtcccctg ctcggtcgtg aagcccaagt cggcctggcc
     1081 cgattcgacg gcctgcggca cctgctcgat ccgcagctcg agcagcctca gctgcacctg
     1141 ctggtgggct ttttcgaatt ccacgatcgg ccgcggcagg tcctcgacga gaattcgctg
     1201 cgtggtggcg atcgtcagcc gcgacgtttc ttgctccagg gcctcggcca cgtttcgctt
     1261 gaaggcccgc acgccctgca ccagcggcgc ggcgagcgtc accacgcggc ggccctcttc
     1321 cgtcaggcgg cagccgcggg cgtgccgctc gatcaggctc acgcccaggt cgcgctgcag
     1381 cgaatggacc tgctcccaga ccgtcggctg cgacagcccg agcgagcggg ccgcggcggt
     1441 caggctgccc agccgggccg tctcgcaaaa gctgcggagc tgctgcagct ggatttcttt
     1501 gtacgcgtga cgcggcgtgg aattggccat tgtgcttcgt atcgcgtagc cgaattcgcc
     1561 agaattcgga atcccttccc gccgtccgaa ctctggcgag ttcggctact ggcatcctat
     1621 aggctggacc tagttcccat caagaaagaa ttcggccggg actttgtgga cttggccggc
     1681 cgcggtgggc tagtctgcaa gcctggaggg ctgccgcatg gcgtttcgct gtttggcgtg
     1741 tttcgtcggc ctggtgctgt tctttcccct ggccgcgcgg gccgagtatc ccttcgtctt
     1801 tcgcgacgtc ggcgccagcg ccggagtgtt gcccgcggcc gccggcatcc gcggccacgg
     1861 cgcgggctgg ggcgacgtgg acggcgacgg ctggctcgac ctgtacgtcg ccacgttcca
     1921 caacgacggc tcgcaggcca acttgttctt ccgcaaccag aacggcaagt tcgagcagga
     1981 ctcgcagccg agcctgaaga tttccgcccg cggcacgggc gtcctgttcg ccgacctcga
     2041 caacgacggc gacctcgatc tgtatctggg cagcatgccc tcgcccgccg gcagccggct
     2101 ggccacgaag gaaggccatc cgatgctggg caaccagctg ctccgcaacg acggcggcgg
     2161 gaagttcacg aacatctcgg acgctaacgg cgcctgcccg gcggccttcg gcggccgcag
     2221 cgtgaccgtc ctcgatttcg acggcgacgg actgctcgac atcctgtgcg gcgaagagcc
     2281 ggtctccggc tacaacggct cgccgaccaa aagctcgcgg ttgttccgca acctggggcg
     2341 gctgcagttc gaggacgttt cgcagcaggt cggcctgacc gcgggagtgc ccggcctggg
     2401 cgtcgccgcg gccgatttga ccggcgacgg ctggcccgac ttcttcctcg cgtcgtcggg
     2461 cggcggcaac gtgatgttca tcaacgacgg ccgcggcaag ttccgcgaag cgccgcgcga
     2521 ggtcttcgcc tggcccaccg ccaagggaga caacatggtc tgcggcgtca cgttctgcga
     2581 cgtgaaccgc gacgccctgc ccgacatggt cctggggcag catttctcga acccctggca
     2641 gcagccggtg gccaatcgcc tgtacctcca tcagggcgtg aaggacggcc tgccggtctt
     2701 tgccgacgtg accgagaaag tcggcctcgt gccgctgccg ctcaaggccc cgcacgtcga
     2761 actgcaagac ctggacaacg acggctggcc cgacctgtat tccagccagg tgaagttcgc
     2821 cggcggccag ccgtacccgc tgatcttcaa gaatctgggc gtgaaaggcg gcctgccgca
     2881 gttccgcgag gagatgctgg cagtcaacga ttttccgacg gccgaggacc gggcgatcgg
     2941 ccgctctggc actttgttcg acaaagtcct gaaagagaag aagatcatct acaccgcgcc
     3001 gggtccgacg gccgacttcg acaacgacgg gcgactcgac atgttcctcg ccaattggtg
     3061 gatcgaagcc ccctcgctgc tcctcaagaa cgagacgccc ggcggcaatt ggctcgacct
     3121 gcaggtcgtc ggtccgcccg gggtcaaccg catgggcatc ggcacccgcc tgctgatcta
     3181 tcccgcgggc aaactgggtg acgccgcgtc gctcctcggc tgccgcgaga tcgccgcggg
     3241 ctacggctac gcctcgggcc agccggcgat cgcccacttc ggcctgggcg agctggccag
     3301 cgtcgatatc gaagcgatcc tgccgcacgg caaaggcaag ctgactcgca aagacgtgaa
     3361 ggcgaaccag cgactgacga ttacgcagta aataccggcg atgaatcagc gagcggaaaa
     3421 caagagcaag gcaaccgcgg atttcgcgga tggcgcggat ttgcatcgga gactcccctt
     3481 atcagcgtta tccgcgaaat ccgcggttca tctggccgga attgtcctaa cgctttgctt
     3541 gatcgcttgt gccttcgccc aaacgccgga cgagcgcaag gcgaagaaaa aactcgatcg
     3601 ctatccgctg ccttatccgc cgacgctggc cggtggccag acgcaggtca cggaaaattc
     3661 gcccgcgttt ctggttccgg ggccgaacct gcgcgaggga gtgctggtcg ccaagacgcc
     3721 gccggtcgtc gatttcgcct tctatccaca gcagaattat ccgggcaacc cctggtcgca
     3781 ccgctccgat ggcatcgtcc gcggtgacaa gtactattcg tcgtcgaacg accatctcgc
     3841 gccgcggggc acggcgctgc tctgggaata cgacgcggtc acgaaacagt tccgcctgct
     3901 ctgcgacacg acgaagttcc tggagtcgca gcacgcgttc ccgcccggga tgaactatcg
     3961 ccccggcgag atgcagagcc gcatcgacct gggcagcgac ggctggctct attactgcac
     4021 cgaccgcggc tcgcccacgg tgaccgacga cgcccacggc tacctgggcg aatggattct
     4081 gcggacgcat ccgcagactc tggcgaccga aatcgtcgcc acgcatccga tcgagaagca
     4141 cacgctgccg gcgagcgtcc tcgatccgga gcggatgatc ctctattgcg gcacggccgt
     4201 cggcaaggac gcgccgaacc agaagatcca gttcttcgca ttcgatctca aaagccgcaa
     4261 gctgctcaag gtcgcggacg acggtccgac gcggacgctg atttttgcca aatcgaccgg
     4321 ctgcgtcttc tgggaaggca agcggtacga cccggcgacc aacgagatca cgccggccag
     4381 cgtcccgcac gtccgctcgg cgacggccga gacgccgcag gggatcgtct atggcacgtc
     4441 gggcaccgcc gccgacctgt gggcctttca cgtgaagacg ggcgagctga aacagctcgg
     4501 ctcggcggcc gtcgccagcc aggaatacat cgcctcgatc gacgccgacc ccaccggccg
     4561 ctatttgtac tacgctcccg gcgctcacgg cggcgcagcg cgcgacggca cgcccgtcgt
     4621 gcaatacgac gtgcggaccg gccgcaaaaa ggtcctggct ttcctgcacc agctctttct
     4681 ggacaagcac ggctacaccc ccgacggctg ttttggctcc gccctcgatg aaaaaggcga
     4741 gcggctcttc atctcctggg acggctggcg caaaggccaa ccccgcggct gggaatcgtg
     4801 ctcgctgacg gtgatccata ttcctgcttc ggagcggcca tgatcggtgc tcgccagccg
     4861 cgttggcctc gcattgccat gatcgcgctg gcggt
//
LOCUS       DASRJB010000783         5546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3603551, whole genome shotgun sequence.
ACCESSION   DASRJB010000783 DASRJB010000000
VERSION     DASRJB010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603551"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFB80_20695"
     CDS             complement(<1..246)
                     /locus_tag="VFB80_20695"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN36265.1"
                     /translation="MLRHRLRDDEWELIEDVFPPRAKTGRPPADRRKIVDGIFWILRT
                     GAPWRDVPEEFGKWHTVWDLFDKWNDAGLLDEILRRLR"
     gene            382..2481
                     /gene="pheT"
                     /locus_tag="VFB80_20700"
     CDS             382..2481
                     /gene="pheT"
                     /locus_tag="VFB80_20700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN36266.1"
                     /translation="MIVSWNWLNDLIPLKVTPAELVQRLMMAGLNHEGSEQLGSDWAI
                     DLEVTSNRPDCLGHIGIAREAAVLFNLQLKYSGADLTRARKDSPAASEPHRIADDLRN
                     YTSIEVACPDLCPRYSARVIRGVKISSSPSQMAVRLATVGQPVVNNVVDCTNYVLMEC
                     GQPLHAFDFQKLAGRKIVVRRAKAGEQFQAIDHKTYTLDPEICMIADGQRAVAIGGVM
                     GGADTEVSRQTTDVLIEAAQFAPLSIRATARKLKLHSPSSYRFERGTDPEMVDWASRR
                     CCELILKHAGGELIEGVIDMGTPRPKRPPITLRLDQLKRIIGIDVPPEAVRRILTALG
                     CLEESASGRDFVVVPPSWRRDLSREIDLIEEVARVHGYDKIPEDASVPMVASHKSDAE
                     RVLAKVRQVMNAGGFDEALTASVVPERWSAAFSPWTDAEPLVANTPLLEGADRLRRSL
                     VPSLLDVRRINESLSNPSIELFETARIYVPDRSPHAPREAGVSQGGTIALPREQPTLA
                     AVSGRGFLAAKGVVEALLAALHIDQPLTVADYCHELLTSDRAGLLELGGQRLGFLGDV
                     SPAGQKAFGLRGAATILEIDLSLLQQLAHLNTTYAPQSPYPTIGRDLNLIVPESVRWA
                     DLAVTVRSAAGPALERLEYLDTYRDPQKDGPDTKRLTLSLTLRAADRTLTGPEADAVT
                     SAVVAACQANHAARLLG"
     gene            2577..3404
                     /locus_tag="VFB80_20705"
     CDS             2577..3404
                     /locus_tag="VFB80_20705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN36267.1"
                     /translation="MKTMLNDLVMRLALELSTRSPAFKRFLWRRWYQYLAGYGVADWG
                     FMNYGFAPVNGEAYPLELEAADEPNRYSIQLYHYVAGRVPLSGRDVLEVGCGRGGGAA
                     FLKRYLHPRQMNGVDFSAKAVRFCRQQHRLEGLTFLAGDAEALPLPDSSFDAVVNVES
                     SHCYGSMPAFLAQVRRVLRPGGHFLFADLRSTPDRDRLHEQIIASGLTVLEQEEITAN
                     VVEALRRDSQRKLALLEKHGSPRLMSVFRQFAAIEGSGVYEGFRTRSAVYVRYLMQK"
     gene            3693..>5546
                     /locus_tag="VFB80_20710"
     CDS             3693..>5546
                     /locus_tag="VFB80_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN36268.1"
                     /translation="MKLYPSLILVAVVGCTTAFGQTPKLGDLLKPFEPPTMEQLDQKA
                     KWVQRPVVDAMKLLREKQAKEAPIGTVAQALAAKNVSRQTNAAILSTVGRLPAKDADV
                     NWDAELKRHINFDVKSTNPILFSSVSENDVNQLTAFGIFSFDWQFNPFASSDTAASWQ
                     SSADRLYDKVVLRDDLLWSDGQPITAHDVEFSFKLIMTEAVPVPAVRHGTDKLRLVKA
                     YDDRTVVFFHEDSLSTNDWNLNFPIIPKHIYEQSWASDPTLATSEHHIKFEKNPVSGG
                     LYTITSRTIGSEILLTRRDDYYLHNGKQVRDKPYFKTIRFRVIQEPGVALLGLKGGEV
                     DELILTPQQWRTQTIDDAYYQTCTKAYDTEWVSFHFVWNCKNPMFSDVRARRAMTLAF
                     DHEEMLTKLRYGLDPPASGIFHPASPWAPPGRATFVKRNLDEAEKLLDACGWKDTDGD
                     GIRDKTVDGKKLKFDFGILVASRQDRIDICSLLKQNLKEIGIEVTVRPLDFAVLQQKL
                     QDHSFEAAFGGWGTGTDPDTSENIWGTNQERNYGFYSNPEVDRLFAAGCKEFDKEKRR
                     LIYQKIHTLIAQDQPYTWLFYQNAYYGFRKELRGYMFSPRGPYHYSPGVSSL"
BASE COUNT         1048 a         1825 c         1688 g          985 t
ORIGIN      
        1 tcgcagccgc cgcaggattt cgtccagcag gcccgcgtcg ttccacttgt cgaacaagtc
       61 ccagaccgtg tgccacttgc cgaactcctc aggcacgtcg cgccatggcg cccccgtccg
      121 caagatccag aaaatgccat ccacgatctt tcgtcgatcc gcgggcggcc ggcccgtttt
      181 tgcacgcggc ggaaacacgt cctcaatcaa ttcccattcg tcatccctaa gccgatgtcg
      241 cagcatcctt gcatcctccg tagaaggtcc gatccatcgg cccacagttt gcgccaaacc
      301 ggtcttgcca cggaataccg gttttcagac aaagcctagc actgcctagc cactagcact
      361 agccaccagc acttcctccc catgatcgtt tcctggaact ggctcaacga tctcatcccg
      421 ctcaaggtca caccggccga gctggtgcag cgcctgatga tggcggggct caaccacgag
      481 gggagcgagc agctgggaag cgactgggcg atcgatctgg aggtgaccag caaccggccc
      541 gattgcctcg gccatatcgg catcgctcgc gaggcggccg tgctgttcaa cttgcagctc
      601 aagtatagcg gggccgacct gacgcgggcc cgcaaagact cccccgccgc cagcgagccg
      661 caccgcatcg ccgacgacct gcggaattac accagcatcg aagtcgcctg ccccgacctc
      721 tgcccgcgct atagcgctcg cgtcatccgc ggcgtgaaga tcagctcgtc tccgtcgcag
      781 atggcagtcc gcctggccac cgtcgggcag ccggtggtca acaacgtcgt cgattgcacc
      841 aattacgtgc tgatggagtg cgggcagccg ctgcacgcct tcgactttca gaagctcgcg
      901 ggccgcaaga tcgtggtccg tcgggcgaag gccggcgagc agttccaggc catcgaccac
      961 aagacctaca cgctcgaccc ggagatctgc atgatcgccg acggccagcg agccgtcgcc
     1021 atcggcggcg tcatgggcgg cgccgataca gaagtctcgc ggcagacgac cgacgtgctg
     1081 atcgaggccg cccagttcgc gccgctttcg attcgcgcaa cggcccgcaa gctgaagctc
     1141 cacagccctt cgtcgtaccg cttcgagcgc ggcaccgatc ccgaaatggt cgattgggcc
     1201 agccgccgct gctgcgagct gatcctcaag cacgccggcg gcgagctgat cgagggcgtg
     1261 atcgatatgg gcacgccgcg gcccaagcgg ccgccgatca ccctgcggct ggaccagctc
     1321 aagcggatca tcggcatcga cgtcccgccg gaagcggtgc ggcggatact tacggccctc
     1381 ggctgcctgg aagaatcggc cagcggccgc gattttgtcg tcgtcccgcc cagctggcgg
     1441 cgcgacctct cgcgtgagat cgacctgatc gaagaggtcg cccgcgtgca cggctacgac
     1501 aagattcccg aggatgccag cgtgcccatg gtcgcctcgc acaagtcgga cgccgagcgg
     1561 gtgctggcca aagtccggca ggtgatgaac gccggcggct tcgacgaggc cctcacggcc
     1621 agcgtggttc ccgagcggtg gagcgccgcg ttcagcccct ggaccgacgc cgagccgctg
     1681 gtggcgaaca cgccgctcct ggaaggggcc gaccgcctcc gccgcagcct cgtgccgagc
     1741 ctgctcgacg tgcgccggat caacgaatcg ctgagcaatc cgtcgatcga gctgttcgag
     1801 acggcacgaa tctacgtgcc tgatcgtagt cctcacgctc cgcgcgaggc gggcgtctcg
     1861 caaggcggga cgattgcgtt gccgcgtgag cagccgacgc tggccgccgt cagcggccgg
     1921 ggttttttgg ccgccaaggg cgtcgtcgag gcactgctgg ccgcgctgca catcgaccag
     1981 ccgctgacag tggccgatta ttgccatgaa ttgctgacct ccgatcgcgc cggccttttg
     2041 gagctgggcg gccagcggct gggcttcctc ggcgacgtct cgccagctgg ccagaaagcc
     2101 ttcggcctgc gtggcgcggc gacgattttg gaaatcgacc tctccctgct gcagcaactc
     2161 gcccacctga atacgaccta cgcgccgcag agcccgtatc cgaccatcgg ccgcgatctc
     2221 aacttgatcg tccccgagag cgtgcggtgg gccgacctgg cggtgacggt acgctccgcg
     2281 gccggcccgg cgctggagcg gctcgagtac cttgacacct atcgcgatcc gcagaaggac
     2341 ggcccagaca ccaagcggct gaccctctcg ctcactctgc gggccgccga ccgcacgctc
     2401 accggtcccg aagccgatgc tgtcaccagc gccgtcgttg ccgcctgcca ggcgaaccac
     2461 gccgcccgct tgctgggtta gctcggtgcg ccctggccga ggcacgtccg tgcctctagc
     2521 caaagcagcc gactttcctg caaaatcctg actctgacaa ccgcagcccg cgttttatga
     2581 aaacgatgct caacgatctg gtcatgcgcc tggccctgga actcagcacg cggtcgcctg
     2641 cgttcaagcg gttcttgtgg cggcggtggt atcaatacct ggccggctat ggcgtggccg
     2701 actgggggtt catgaattac ggctttgcgc ccgttaacgg cgaagcttac ccgctggaac
     2761 tcgaagcggc cgacgagccg aaccgctatt cgatccagct ctatcactac gtcgccggcc
     2821 gcgtgccgct atccgggcgc gacgtgctcg aagtgggctg cggccgcggc ggaggcgcgg
     2881 ccttcctcaa gcgctacctg catccccggc agatgaacgg cgtcgatttc tcggccaagg
     2941 ccgtgcggtt ttgccgccag cagcatcgcc tggaagggct cacgttcctg gccggtgatg
     3001 ccgaggcgct gccgctgccc gacagctcgt tcgacgcggt cgtcaacgtc gagtcgtcgc
     3061 attgctatgg ctccatgccc gcatttctcg ctcaggtgcg gcgagtgctg cggccgggcg
     3121 ggcacttcct gttcgccgac ttgcggagca ctcccgaccg cgaccggctg cacgagcaga
     3181 tcatcgcctc cggcctgacc gtgctggagc aggaagagat caccgctaac gtcgtcgagg
     3241 ccctccgccg cgacagccag cgcaagttgg ccctgctcga aaagcacggc agcccgcgcc
     3301 tgatgagcgt ctttcggcag ttcgccgcca tcgagggttc cggcgtttac gaaggcttcc
     3361 gcacccgctc agccgtgtac gtgcgatatc taatgcaaaa gtaggccggg attatgcggg
     3421 cgatgcccgc cggctcccgg aggtcaatcg agtcgactcc tgccccgctt cctttgggcc
     3481 agacgacgct cagtcgcggt cgatgcgcga cagccgatcc ctgatctgct tgccgagttt
     3541 gccgctgggg cctgcggacg gccaaaccag cgctccaccg gttgcgtaga aacgaacgaa
     3601 gtccagtcgc caacgcaggt tgcggcaatt ttgcgaaagg gctagatttg accgggggtc
     3661 gttggcggca gagcttccag aaggaaacat cgatgaaact ctatccgagt ttgatcctcg
     3721 tcgcggtagt gggttgcacg acagcattcg gtcagacccc caagctcggc gacctgctca
     3781 agccgttcga gccgccgacc atggagcagc tcgatcagaa agccaagtgg gtgcagcggc
     3841 cggtggtgga cgctatgaag ctgttgcggg agaagcaggc gaaggaagcg cccatcggta
     3901 cggttgccca agcgctagcg gccaagaatg tctcccggca gaccaacgcc gcgatcctga
     3961 gcacggtcgg ccggctgccg gccaaggacg ccgacgtgaa ctgggacgcc gagctaaagc
     4021 ggcacatcaa cttcgacgtc aagagcacca atccgatcct gtttagttcg gtgtcagaga
     4081 acgacgtcaa ccagttgacg gcattcggga tcttttcgtt cgactggcag ttcaatccct
     4141 ttgccagcag cgacaccgcg gcgtcctggc agtcgagcgc ggaccgcctg tacgacaagg
     4201 tcgtattgcg cgacgacctg ctctggtcgg acggccagcc gatcacggcg cacgacgtgg
     4261 aattcagctt caagctgatt atgaccgagg ccgttccggt tcccgcggtc cgccacggca
     4321 cggacaaatt gaggctcgtc aaagcctatg atgaccgcac cgtggtcttc tttcacgaag
     4381 attccctatc gacaaacgac tggaatctca acttcccgat cattcccaag catatctatg
     4441 agcagagctg ggcctccgat cctacgctgg ccacgagcga gcatcatatc aagtttgaga
     4501 agaatcctgt ttcgggcggt ctttatacca tcaccagccg cacaatcggc agcgagatcc
     4561 tgctgacgcg ccgcgacgat tattacctgc acaatggcaa gcaggtgcgc gataagccgt
     4621 acttcaagac gattcgcttt cgggtgattc aagagccggg cgtcgcgctg ttgggcctca
     4681 aaggcggcga agtcgatgag ctaatcctga caccgcagca gtggcggact caaacgatcg
     4741 atgacgcgta ttatcagacc tgcacgaagg cctatgacac ggaatgggtc agcttccact
     4801 tcgtctggaa ctgcaaaaac ccgatgttct ccgacgtccg cgcgcggcgg gcgatgacgc
     4861 tggccttcga ccacgaagag atgctgacca agctgcggta tggattggac ccgccggcca
     4921 gcggcatctt ccacccggcg tcaccttggg cgccgcctgg gcgggccacc ttcgtcaagc
     4981 ggaacctgga cgaggccgag aagctgctcg acgcctgtgg ctggaaggac accgatggcg
     5041 acggcatccg agacaagacc gtggacggca aaaagctcaa gttcgacttt ggcattctgg
     5101 tcgccagccg gcaggatcgc atcgatatct gcagcctgct gaagcagaac ctgaaagaaa
     5161 tcgggatcga agtcaccgtc cgtccgctag attttgccgt gctgcagcag aagcttcagg
     5221 atcacagctt cgaggcggcc tttgggggct ggggcacggg caccgacccc gatacgagcg
     5281 aaaacatctg gggaaccaat caggaacgca attatggctt ctattccaat cctgaggtcg
     5341 atcgactgtt tgccgccggc tgcaaggaat tcgacaaaga aaagcgacgg ctgatctatc
     5401 agaagattca tacgttgatt gcccaggatc aaccctatac atggctcttc tatcagaatg
     5461 cctattatgg gttccgcaag gaactgcgcg gatatatgtt cagcccgcgc ggaccctatc
     5521 actattcgcc aggtgtcagc agtctg
//
LOCUS       DASRJB010000784         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3333800, whole genome shotgun sequence.
ACCESSION   DASRJB010000784 DASRJB010000000
VERSION     DASRJB010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333800"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1707
                     /locus_tag="VFB80_20715"
     CDS             289..1707
                     /locus_tag="VFB80_20715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36269.1"
                     /translation="MRYSREICVAAWIALQLACWAAPQRPNIVVLIADDLGYGELGCY
                     GGREIPTPNLDALAAAGVRFTSGYATAPFCAASRAGLLTGRYQTRFGFEFNPIGAKNA
                     DPQIGLPASERTIALRLSDAGYATALIGKWHLGGTAEFHPQRRGFDEFFGFLHEGHYY
                     VPPPWKGVTTWLRRTALPDGGQGRWTSQGGRVVVSTHMGHREPPYDADNPILRASQPV
                     DERANLTDAFSREASDFIERHRSQPFFLVVAFNAVHSPLQGDDVHLARFAHIPDIHRR
                     IFAALLAHLDDAVGQVVAKLKAEGLTENTLVVFLSDNGGPTRELTSSNAPLRGGKGEL
                     LEGGIRVPFLASWEGHLPAGRATDAPISSLDIGATALAAAGIPLAADKLDGIDLLPMV
                     RQPQGTLSPRTLFWRVGQRHALRRGDWKLIRDGGRPWRLYNLAADLGETTDLAESQPE
                     LVVELAALWDRWNAEQAEPRWK"
     gene            1725..>2208
                     /locus_tag="VFB80_20720"
     CDS             1725..>2208
                     /locus_tag="VFB80_20720"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN36270.1"
                     /translation="MPLAHKTATVEADSPLAEQRADRLVQQLCGLSRSQVTGLFDHGC
                     VRIGGAVCTQPAQRLKAGDRVEVKYDPHQRYHPRTRPRRNLGFEIVFEDPQLLVVNKP
                     AGLLSVPTARDETNTLVAKATEYVVHVSKGRGAFTVHRLDRGVSGLLVLGKSQEIVGQ
                     L"
BASE COUNT          372 a          735 c          742 g          359 t
ORIGIN      
        1 cgcgccggcc gcctgcccgg ccggcctgga ggctggggcg aagaaagcga atgcatgaac
       61 attggtacgc tatttgcagt atagttcccg ttggcgcaaa ggctgccaat atggcggtca
      121 gggccgcgcg ggcgcacgca acgtagccgt tctcaaaggc ttattaagta ctacttaagg
      181 gttttcttga acgtcgccac aagtccatga tctggcaaga gttgcgacaa gattagcggc
      241 cgcagggcag ttgcagaccg ctcaacgaag ggggcgcagg aatcgacgat gagatactct
      301 cgggagattt gcgtggcagc ctggatcgcg ctgcaactgg cctgttgggc tgccccccag
      361 cggccgaata tcgtcgtgct gatcgccgac gacctgggct atggcgaact gggctgttac
      421 ggcggccgcg agattccgac gcccaacctc gacgccctgg ccgccgccgg cgtgcggttc
      481 actagcggct acgcgaccgc gccgttttgc gcggcatcgc gggccgggct gctcacgggg
      541 cgataccaga cgcggtttgg cttcgaattc aatccgatcg gggccaagaa cgccgacccg
      601 cagatcggcc tgccggcgag cgagcggacg atcgctctcc gcctcagcga cgccggctat
      661 gccacggcct tgatcggcaa atggcacctg ggcggcacgg ccgagtttca tccgcagcgg
      721 cgcggcttcg acgagttctt cggcttcctg cacgagggcc attactacgt gccgccgccg
      781 tggaagggcg tgacgacgtg gctgcggcgg acggccctgc ccgacggcgg ccagggccgc
      841 tggacctcgc agggcggccg cgtcgtcgtg agcacgcaca tgggccatcg cgaaccgccc
      901 tacgacgccg acaatccaat tctccgcgcg agccagccag tcgatgaacg agcgaatctc
      961 accgatgcct tttcgcgtga agcctctgac ttcatcgagc ggcaccggtc gcagcccttc
     1021 ttcctggtcg tggctttcaa cgcggtgcac agcccgctgc agggcgacga tgtgcacctg
     1081 gccaggttcg cacacattcc cgacattcac cgccgtatct tcgcggctct gctcgcgcac
     1141 ttggacgacg ccgtcggaca ggtggtcgcg aagctgaaag ccgagggcct gaccgagaac
     1201 acgctggtcg ttttcctcag cgacaacggc gggccgaccc gcgagctgac ctccagcaat
     1261 gccccgctcc gcggcggcaa gggcgaactg ctggagggtg gcatccgcgt gccatttctg
     1321 gcgtcctggg aagggcattt gcccgccggt cgcgcaactg acgcgcccat cagctcgctc
     1381 gacatcggcg cgacggccct ggcggccgcc ggaattccgc tcgccgcgga caagctcgac
     1441 ggtatcgacc tccttcccat ggtgcggcag ccgcagggca cgctttcgcc gcgcacgctg
     1501 ttctggcggg tcggtcagcg gcacgctctc cgccgcggcg actggaagct gatccgcgac
     1561 ggcgggcggc cgtggcggct ctacaacctg gccgccgatc tcggcgaaac taccgatctg
     1621 gcggagagcc agccggagct ggttgtcgag ctggccgccc tgtgggaccg ctggaacgcc
     1681 gagcaggccg agccgcgctg gaagtaaccc caaagctgcg catcatgccg ctggctcaca
     1741 agactgccac cgtcgaagcc gattcaccat tggccgagca gcgcgcagat cgcctggtgc
     1801 agcagttgtg cggcctctca cgatcgcagg tgacggggct cttcgatcac ggctgcgtgc
     1861 gaattggcgg cgcggtctgc acgcagccgg cccagcggct gaaggcgggc gaccgcgtgg
     1921 aagtgaaata cgatccacac cagcggtatc atccgcggac ccggccgcgg cggaatctgg
     1981 gcttcgagat cgtcttcgaa gatccgcagc tgctggtcgt gaacaagccc gcggggctgc
     2041 tcagcgtgcc gaccgcccgc gacgagacga acacgctcgt ggccaaggcg accgaatacg
     2101 tggtgcacgt cagcaagggc cgcggggcct tcacggtgca tcggctcgac cgcggcgtct
     2161 cgggcctctt ggtgctggga aagtcgcagg agatcgtcgg ccagctca
//
LOCUS       DASRJB010000785         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1355539, whole genome shotgun sequence.
ACCESSION   DASRJB010000785 DASRJB010000000
VERSION     DASRJB010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355539"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFB80_20725"
     CDS             complement(<1..213)
                     /locus_tag="VFB80_20725"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN36271.1"
                     /translation="MLYLFLFGVAIGIVSGLLGIGGGILLVPGLMLLFQFSQQEAQGT
                     SLAVLIPPIGIFAALVYIQNGDVKLPV"
     gene            545..991
                     /locus_tag="VFB80_20730"
     CDS             545..991
                     /locus_tag="VFB80_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN36272.1"
                     /translation="MMTLRFSPLSDLRDEMDRMRNHFENLFGQARGQRPGLDPTGFPA
                     LNAWEEEGNFYVEAELPGLALEDLEISLSEADTLSIKGQRKAPEREGGTWHRRERAFG
                     SFERTVKLPAAVDAERVEASLKLGVLTVKLPKAPELQPRKIEVKAL"
     gene            1042..1431
                     /locus_tag="VFB80_20735"
     CDS             1042..1431
                     /locus_tag="VFB80_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN36273.1"
                     /translation="MNDTMECTNSGGCEVARPQSKVTFTPRFDIWENDQEYVLAGDLP
                     GVDPQELEIRYENQELAIHGKVAPRDGEARYFAVEYGVGDFHRGFRLGDQIDGSAIAA
                     ELKGGVLTVRLPKRAEARPRKNTVTAG"
     gene            complement(1428..3971)
                     /locus_tag="VFB80_20740"
     CDS             complement(1428..3971)
                     /locus_tag="VFB80_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36274.1"
                     /translation="MKLEGTKYTKGAIRRIACFVLFVSSCLAACVALSRAADSSDAKF
                     LSGLRERRLFELAEVYCADRLRSAERNGAAERELTLELIRTLALHAAHSPPQGRAALL
                     DRARRSAADYLRRAPLPPRAILVRVQDALTPLAQGELGRQEFEAGLVAADQLEPARQA
                     LREATALLEALDKELAREIPLRRRTPPRAGELNADELIGLHQDVQRQLARGQRNRALL
                     AGPGSDDRRALLLAAIETLKRPLAQLSDDDPLRPQVQLDQAECLRLAGQAAEAGELAA
                     GFDQEKIAPAVRWRARAELIRVALAQNNLTGAQRLMDAGQSATGQSSAEFDFARFEAL
                     LALSGSASATQARQYQEEAAAAAKSIDDSHGPYWGRRADQLLIAALPRGAGASAQLLA
                     RKADGLYLKQDFAQAVAAYDDASAAARSAGQTDAAFDLAAKAALALQQRGQHAAAADR
                     LRKLAKELPTHPRAPPAHLLAVWNAAQLAHGQPEAAEFYAALVREHLADWPQAASASQ
                     ARLWLGKLAELRGDFAAAIDAYRGVPRSSEHFDAAVLALADCWRQRLAKLAAAGQPTA
                     QPLAEALGQLTAVWSGREKSAAWSAAERAAALAQAELILAFEPSRAAAAEQSLRAVLA
                     GAGDVQEEWQLTAHSQLVVALAGQPGREREAEDVLRQLRAASPVQLLAVLEGLAQAAS
                     GGTSQSRPKIAAVQLAALKLLEQDRQQLLPAEQLRLSTIEAEALATSGRRDEALAALE
                     KLAQAIPDSGVVHERYAALLLDSNDRSQLEQALAQWRIIASRSQPHGPRWLQAKYSIA
                     VAQLKLGDRERAATLLQYILAVPPGLKGSPWEAKYNELLRQGQQPATAP"
     gene            complement(3968..4354)
                     /locus_tag="VFB80_20745"
     CDS             complement(3968..4354)
                     /locus_tag="VFB80_20745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN36275.1"
                     /translation="MRIPSRPVSGELSPSIAPLIGVVILIALLAPFVDRPAKRPAQSP
                     PPPEADFDAIVIRVSPGPRWQVNGVPMGSLDALRQALTPVAKIKRDAPIIIETLGDVP
                     PSDITEVFDLMRQIGFERVQRRDAAK"
     gene            complement(4329..>4559)
                     /locus_tag="VFB80_20750"
     CDS             complement(4329..>4559)
                     /locus_tag="VFB80_20750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN36276.1"
                     /translation="FARGLRSIPGHEATGIGLTEPILVPVWVVLAWGDRPAWWTLVGG
                     GLILVGLAVRYLGGPAEETAVKSGDANSQPAS"
BASE COUNT          740 a         1525 c         1593 g          701 t
ORIGIN      
        1 gaccggcagc ttgacgtcgc cattctggat gtaaacgagc gccgcaaaga tgccgatggg
       61 cggaatcagc acagcgaggc tcgtgccctg agcttcctgc tggctgaact gaaagagcag
      121 catcaggccc ggcaccagca ggatgccgcc gccgatgccc aacaggccgc taacgatgcc
      181 aatggcgacg ccgaacagaa agagataaag cacggtggac tccgggtcga aaaatcgaac
      241 gagcgttcat tgtggagtcc gcggcgtacc gtggaaactc gcgattgtca atttggcagg
      301 cgcccagcgg cggaaaatgt tttcgccctc gcgagccttg cggcgcgagt gcccgcgatc
      361 gccgcaaatc tcgaagcgaa ttcgagttgc gatccaatcg caggtcctcg attggcgatt
      421 ggcacgctgg ttgcaatttc cacttccgcc ggcggctgcc tacgtcgtcg cgggaacagg
      481 aagtatcgat tgtgccgccc gttggcaggc ggccttaaaa ccggattttt gcgaggaggt
      541 atcgatgatg acgctgcgtt tttctccact ctccgacctg cgggacgaaa tggaccgcat
      601 gcggaatcac tttgagaacc tgttcgggca ggcccgcggc cagcggccgg ggctcgatcc
      661 caccggcttt ccggcgctga acgcctggga agaggaaggg aatttctacg tcgaggccga
      721 actgccggga ctggcgctgg aagacctgga gatctcgctg agcgaggccg acacgctttc
      781 gatcaagggc cagcggaagg ccccggagcg cgaaggcggc acctggcacc gccgcgagcg
      841 ggcgttcggc tcgttcgagc ggacggtgaa gctgccggcc gcggttgatg ccgagcgcgt
      901 cgaggcgagc ctgaagctgg gcgtgctcac ggtcaagctg ccgaaggcgc cggaacttca
      961 gccgcggaag atcgaggtca aggccctgta agccgcgtac cgcaagcggc cagcaaaatc
     1021 acatacaagg aacaaatcac aatgaatgac acgatggaat gcaccaatag cggcgggtgc
     1081 gaggtcgccc ggccgcagag caaggtcacg tttacgccgc ggttcgacat ttgggagaac
     1141 gaccaggagt acgtcctggc cggcgatctg cccggcgtcg atccgcagga gctggagatc
     1201 cgctacgaga accaggagct ggcgatccac ggcaaggtcg ccccgcgcga cggcgaggcc
     1261 cgctatttcg ccgtcgagta cggcgtcggc gatttccacc gcggcttccg gctgggcgac
     1321 cagatcgacg gctcggcgat cgccgccgag ttgaagggcg gcgtgctcac cgtgcgtctg
     1381 cccaagcggg ccgaggcccg cccgcggaag aacaccgtga cggccggcta gggcgcggtc
     1441 gccggctgct ggccctgccg cagcagctcg ttgtatttcg cctcccaggg actgcctttg
     1501 agcccgggag gcaccgccag aatgtattgc agcaacgttg ctgcccgctc tcggtcgccg
     1561 agcttcagct gcgccacggc gatcgagtat tttgcttgta gccagcgcgg gccgtgcggc
     1621 tgcgagcggc tggcgatgat ccgccactga gcgagcgcct gctcgagctg cgaacggtcg
     1681 ttcgagtcga gcaggagggc cgcgtatcgc tcgtggacca cgccgctgtc ggggatcgcc
     1741 tgggccagct tttccagcgc ggccagggcc tcgtctcggc ggccagacgt ggcgagggct
     1801 tcggcctcga tcgtgctcag ccggagctgc tcggcgggca gcagctgctg gcgatcctgc
     1861 tccagcaatt tcagggctgc gagctgaacc gccgcgatct tgggccgcga ctggctcgtg
     1921 ccgccagagg cggcctgcgc cagtccttcg agcacggcaa gcaactgaac cggtgaggcc
     1981 gcgcggagtt ggcgcagcac gtcttccgct tcgcgttcgc ggcccggttg tcctgcgaga
     2041 gccaccacga gctgcgaatg agccgtcaat tgccattcct cttgcacgtc gcctgcgccg
     2101 gcgaggactg cgcggagcga ctgttccgcc gcggcggcgc gggagggctc gaaggccagg
     2161 atcagttccg cctgagccag cgccgcggcg cgttccgcgg ccgaccacgc ggcggatttc
     2221 tcgcggcccg accagactgc cgtcaactgc ccgagtgctt cggccagcgg ctgggccgtc
     2281 ggctgaccgg cggcggcaag ttttgccagc cgctggcgcc agcaatcggc cagggcgagc
     2341 acggcggcat cgaagtgctc ggacgagcgc ggcacaccgc gataggcgtc aatggcagca
     2401 gcgaagtctc cccgcaattc ggccagcttg ccgagccaca gccgtgcctg gctggccgac
     2461 gcggcctgcg gccagtcggc caggtgctcg cggacgagcg cggcgtagaa ctccgcggcc
     2521 tcgggctgcc cgtgggccag ctgcgcggcg ttccagaccg ccagcaggtg cgcgggcggc
     2581 gctcgcgggt gagtcggcaa ctccttcgca agcttccgca gccgatcggc cgcggcggcg
     2641 tgctggccgc gctgctgcag ggcgagcgcc gccttggcgg ccaggtcgaa tgccgcgtcg
     2701 gtctggcccg cggaccgggc cgcggccgag gcatcgtcgt aagccgcgac cgcctgtgcg
     2761 aagtcctgtt tcaaatacag cccgtcggcc ttgcgggcca gcaactgagc gctggccccc
     2821 gcgccgcgcg gcagcgcggc aatcagcaac tgatcggcgc ggcggcccca atacgggccg
     2881 tggctgtcat cgatcgactt tgcggcagcg gcggcctctt cctgatactg acgggcctgc
     2941 gtcgcactgg ccgagccgga cagagccagc agcgcttcga accgcgcgaa gtcgaattcg
     3001 gcagacgact ggccggtggc gctctggccg gcatccatca gccgctgggc accggtcaga
     3061 ttgttctgcg ccagcgcgac gcggatcagc tcagcccggg ctcgccagcg gaccgctggc
     3121 gcgatttttt cctgatcgaa ccccgccgcc aattcgcccg cttcggccgc ctgccccgcc
     3181 aggcggaggc attcagcctg gtcgagctgt acctgcggcc gcagcggatc gtcgtcgctc
     3241 aattgagcca gcggccgctt gagcgtctcg atcgcagcca gaagcagcgc gcggcggtcg
     3301 tcgctgcccg gcccggccag cagcgcccgg ttgcgctggc cgcgggccag ctgccgctgc
     3361 acgtcctggt ggaggccgat cagctcgtcg gcgttcagct cgccggctcg cggcggcgtg
     3421 cggcggcgga gcggaatctc gcgggccagc tccttgtcga gcgcttccag cagcgcagtt
     3481 gcctcgcgga gcgcttgccg ggccggctcg agctggtcgg cggcgacgag gccggcctcg
     3541 aattcctggc ggcccagttc gccctgagcc agcggcgtga gcgcgtcttg aacgcggacg
     3601 agaatcgccc gcggcggcaa gggcgcgcgg cgaagatagt ccgcggccga ccggcgggca
     3661 cggtccaaga gcgccgcgcg accctgcggc ggcgagtgcg cggcgtgcaa ggccagcgtg
     3721 cgaatcagct ccagcgtcag ttctcgttcc gccgcgccat tccgctcggc cgatctcagc
     3781 cgatcggcgc agtagacctc ggccagctca aacagccgcc gttcgcgcag gccgctgaga
     3841 aacttcgcgt ccgaagagtc ggcagctcgc gacagggcga cacaggcagc taaacacgaa
     3901 gacacgaaga gcacgaagca ggcgatgcgg cgaattgcac ccttcgtgta tttcgtacct
     3961 tcgagtttca ctttgccgcg tcccttcgct gtactcgctc gaagccgatc tgccgcatga
     4021 ggtcgaagac ttcggtgatg tctgacggcg gcacgtcgcc gagagtttcg atgatgatcg
     4081 gagcgtcgcg cttgattttg gcgaccggcg tcagcgcttg ccgcagtgcg tcgagcgagc
     4141 ccatgggcac gccgttgact tgccagcgcg ggcccggcga aacccggatg acgatggcgt
     4201 cgaagtcggc ttcgggaggc ggcggcgact gcgccgggcg ttttgccggc cggtccacga
     4261 acggggccag caaggcgatc aggatgacga cgccgatcag cggcgcgatg ctggggctca
     4321 gctcgccgct aactggccgg ctgggaattc gcatcgccag attttaccgc ggtttcttcc
     4381 gcggggccgc ccagatagcg gaccgccagg cccacgagaa tcagcccgcc cccgacgagc
     4441 gtccaccagg ccggccggtc gccccaggcc agcacaaccc acaccgggac gagaatcggc
     4501 tccgtcaggc cgatgcccgt cgcctcgtgg ccgggaatgc tccgcaggcc gcgggcgaa
//
LOCUS       DASRJB010000786         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_519513, whole genome shotgun sequence.
ACCESSION   DASRJB010000786 DASRJB010000000
VERSION     DASRJB010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519513"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFB80_20755"
     CDS             complement(<1..761)
                     /locus_tag="VFB80_20755"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZN36277.1"
                     /translation="MPALLVAAVLSAISAAEPPASSVDGDFAPAVALAQKRTVKIFGA
                     GIGRTPGYATGLVVGPQGEILTAQGIFLNADNLRATLPDGTTHMATVVRRSAELQAAL
                     LKIDAATPEHFSISDAAKGERGDWILAVSNAFKVADGVEPLSVNVGVLSVRMPIDARR
                     GFNDFPYQADAWLYDAITSNPGAEGGAVITADGRLVGMVGRVIESKSTGTRLNYAVPA
                     DLLARFAAGEETQPMAVARPGTKSDLGIRLFALGGR"
     gene            complement(869..2686)
                     /locus_tag="VFB80_20760"
     CDS             complement(869..2686)
                     /locus_tag="VFB80_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN36278.1"
                     /translation="MLLVAISQAAEGQPVIAPAELAAVKAAEAARVQAIDSVYGAVVA
                     IYGNDRGGGGSGVIYDPAGYALTNHHVIGGAGLEGWGGLADGKLYRWKLVGTDPGGDV
                     AIIKLEGKDKFPAAPLGDSDAVRVGDWAFAAGNPFTLAEDQRPTVTLGIVSGVKRFQE
                     GSGLNQLVYGNCIQVDSSINPGNSGGPLFNLRGQIIGINGRGSFEERGRVNVGLGYAI
                     SSNQIKNFIPELLSTKIARHGTLDAIFGNRTAGVICFTVNLDSPAARAGLALGDKLVA
                     FEGEPIETANQFTNLISTYPEGWPVELVVEREGAKKTIHTRLSYLPYEPIVRPGSKPP
                     EKPKPEEKPAEPPKPGEPMPPMPPPEFKIEQRPKLPLGDAGKIRDAKLNRAIAEQIVA
                     RWRQSAGATEKDSFRLQAEIRRGAEVVGEQTLSLASGGRIRADYTVDGRRTIIATDGK
                     TCWNDLPDKDPQTVLRGKALLDPHFAQGIALAALLGTDSLTNWGELALDGSDKAGGRL
                     CYRLSVTDAETSEQLFVWLSVHDAGGQPRIELVKTGVGLDDDEPIAGTLFGGDLVLRG
                     FQSPRVLVRGLAENKELTAVFKSVELTGEILAAEFEPKP"
     gene            complement(2847..4046)
                     /locus_tag="VFB80_20765"
     CDS             complement(2847..4046)
                     /locus_tag="VFB80_20765"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HZN36279.1"
                     /translation="MRPTSPHLCLAALFALLGFAQAQVPAKPTAPQLVLLDGSAVPLQ
                     SLQIAAGKLSGEGVPADLTLDDLRRIEVAPVSAVTEKPAMIVELRGGGKLLAKGVTLG
                     DDKCQVAWSGETPLALAIDLVRSIRFDPAAASPDFEKAVAAPSAELDRIFLKDELGKL
                     SSVTGLIDKLTEEQLTFEVGGQSRSVPRSRLFGVVVAQPAAADPPARCLLSLQGGSLL
                     GAYSIELAGGSATLSFAGGGKTALPWALVARITISSSRVAYLSDLKPLAEEQTALVTL
                     PRPAQRDKNVLSKPLTLGTKTYDKGLGVHARSSLTFAAEGKWDTLAATIGLDAAAAGK
                     GDCVFSVLGDGQPVFSRRVKGGDAAQEISVAVAGKQEVTLLVEPGEGLDLADLADWCE
                     VRFIKNR"
     gene            complement(4167..5210)
                     /locus_tag="VFB80_20770"
     CDS             complement(4167..5210)
                     /locus_tag="VFB80_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36280.1"
                     /translation="MARRWFSAIVCFCCLTAGALAADANKVLDDFTASVEKSGATAEQ
                     KRVVAELVKQLRQTPEDRAAAITESLRILYPDYRSALTALGEDNLGAAIVGLTKLREN
                     ADPYLAADASYYLGRAYLLDERFEDALPLLSDLEGKWADKTATGAEVLFLRGVAEVAL
                     LRHKEAMATLEKFLATYPDAPERMRVGAFRQLEQLKLFQEGTLSDVHLRMEFSRRKLS
                     LEDTGTETRQQQDKIIEILAKLIKEAEERECNCKGGGKGQGQKPGSKPGEGDGQAQGQ
                     GSQSGNSGGGSKGIDSDTVKRLHRGGPQSPWSQLRDKDRDPVYSAIKEKFPARYQQLI
                     EQYYKSFQDDSEG"
     gene            complement(5308..>6508)
                     /locus_tag="VFB80_20775"
     CDS             complement(5308..>6508)
                     /locus_tag="VFB80_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36281.1"
                     /translation="VPPAPPNRPAEPGEKPLGQAIENQKQAEKNLQEGKGKNAQQDEE
                     NALANLQRALNELKNEAQRIASLPPEAFEKLAKKQDDIANQTAQVEQKMQEAAAKAAA
                     QGGGGGGGGGGSGKPQPGQQKVQQAQKSMQQASGGLRKQEPADANRQQGKAIKELEEA
                     LREIEERLAQLREETQLEKLARLEARFREMLSIQQRLTVQTAALEKKRGENGGTLARS
                     DRNSVRAIGDDERRMEAVRSETEAKDAGLAGKAQQALEIILDDGTSVVFPDVVGQLRD
                     DLITVGGLLADNMRTDTYTASMQKEIETTLEELIEALQQAQQQKESMGGGGGGGGEPP
                     LLPNSAELKLLRAAQLRVNRRTTSLEQARPKNAPLDDVLKGETQKIAQRQAEIGEMTI
                     RILERGP"
BASE COUNT          952 a         2171 c         2124 g         1261 t
ORIGIN      
        1 cggccgccga gggcaaagag gcggatgccg aggtcggact tcgtgccggg ccgggcgacg
       61 gccattggct gcgtctcctc gccggctgcg aatctggcga gcaagtccgc cgggacggcg
      121 tagttcagcc gcgtgccagt gctcttgctc tcgatgacgc ggccgaccat gccgaccagc
      181 cgtccgtcgg cggtgatgac cgcgccgcct tccgcgccag ggttgctggt gatcgcgtcg
      241 tagagccagg cgtcggcctg gtagggaaag tcgttgaagc cgcggcgggc gtcgatcggc
      301 attcgcacgc tcagcacgcc cacgttgaca ctgagcggct ccacgccgtc ggcgaccttg
      361 aaggcgttgc tgacggccag aatccagtcg ccccgctcgc ccttggccgc gtcggaaatc
      421 gaaaagtgct cgggtgtggc ggcgtcgatc ttgagcaagg ccgcctgcag ctcggcgctg
      481 cggcggacga ccgtcgccat gtgcgtcgtg ccgtcgggca aggtggcgcg gagattgtcg
      541 gcattcagga agatgccctg ggcggtgagg atttctccct gcggccccac gaccagtccg
      601 gtcgcatacc ccggcgtgcg gccgatcccc gccccgaaga ttttcaccgt ccgcttctgg
      661 gccagggcca cggcaggggc gaagtcgcca tccacggagc tggccggcgg ctcggcggcg
      721 gagatggcag agagaacggc cgcgactagc agcgcaggca atgcgagttt cgcacttcgt
      781 actccgtacc aagtactgag tactgagtac caaatacaag gtaccaagta cggacgattg
      841 accaacctcg tgctcatttc ccgcctgctc atggcttcgg ctcaaactcc gctgccagaa
      901 tctctcctgt caactcgacg ctcttgaaca ccgccgtcag ttccttgttt tccgccagcc
      961 cccgaaccag cacgcgcggc gactgaaatc ctcgcaacac cagatcgccg ccgaacagcg
     1021 tacccgcgat cggctcatcg tcgtccaggc ccacgccggt cttaaccagc tcgatccgcg
     1081 gctggccccc agcatcgtga accgagagcc agacaaagag ctgctcgctc gtttccgcat
     1141 cggtgaccga gaggcggtag cagagccggc cgccggcctt gtcgctgccg tccaaggcga
     1201 gctcacccca attggtcaga gaatcggttc ccaagagcgc ggccaaggcg atgccctggg
     1261 cgaaatgcgg atcgagcagg gccttgccgc ggaggacagt ctgcgggtcc ttgtccggca
     1321 aatcgttcca gcaggtcttg ccgtcggtgg cgataatggt gcggcggccg tccaccgtgt
     1381 agtcggcgcg aatccgcccg ccggaagcca gcgacagcgt ttgctcgccg accacttcgg
     1441 ccccgcggcg gatttccgct tgcagtcgga aggagtcctt ttcagttgcc ccggccgact
     1501 gacgccagcg ggcgacgatc tgttccgcga tggcgcggtt cagcttcgcg tcacgtatct
     1561 tgcccgcatc gcccagcggc agcttggggc gctgctcgat tttgaattcc ggtggcggca
     1621 tcggcggcat gggctcgccg ggcttcggcg gctcggcggg tttctcctcc ggcttgggtt
     1681 tctcgggcgg cttgctgccg ggcctcacga tcggctcgta gggcagatag gagagccgcg
     1741 tgtgaatcgt cttcttggcc ccttcgcgct cgacgaccag ctccaccggc cagccttcgg
     1801 gataggtgct gatcaggttc gtgaactggt tcgcagtctc gatcggctcg ccttcgaacg
     1861 cgaccagctt gtcgccgagg gccagcccgg ccctcgcggc gggcgaatcg agattgaccg
     1921 tgaagcagat cacgcccgcc gtgcggttgc cgaaaatcgc gtcgagcgtg ccgtgccggg
     1981 cgatcttcgt gctcaagagc tcggggatga aattcttgat ctggttcgac gagatggcgt
     2041 aacccaggcc gacgttcacg cggccgcgtt cctcaaaact gccgcggccg ttgatgccga
     2101 tgatctggcc gcgaaggttg aatagcgggc cgcccgagtt gccggggttg atcgagctgt
     2161 ccacctggat gcagttgccg tagacgagct gattcaatcc cgaaccttcc tggaaccgct
     2221 tgacgccgct gacgatgccc agcgtcaccg tcggccgctg gtcttcggcc agagtgaagg
     2281 ggttgcccgc ggcaaaagcc cagtccccga cgcggacggc gtcgctgtcg cccagagggg
     2341 ccgccgggaa cttgtccttc ccttcgagct tgatgatcgc cacgtcgccg cccggatcgg
     2401 tgccgacgag cttccagcgg tagagcttgc cgtcagccag cccgccccag ccttccagcc
     2461 ccgcgccgcc gatcacgtga tggttcgtca gggcatagcc ggccggatcg tagatcactc
     2521 cgctgccgcc gcccccccgg tcgttgccgt agatggcgac gacggctcca tagaccgagt
     2581 cgatcgcctg aacgcgggcc gcttctgccg ctttgaccgc ggccagctca gccggggcga
     2641 tgacgggctg gccttcggcc gcctgtgaaa tggccaccag aagcatgcag aatacggtgg
     2701 tgcatgcatg catgcatatt ccaacgtttt cccgccgctg ccgccttgcc ataactggtt
     2761 tgctgataat cagttgcgtc agtttggcga gcatgccgtt atgcagttta tgcaagttaa
     2821 gcataggaga gctcgcttct gcatgactac cgattcttga taaaccgcac ttcgcaccaa
     2881 tcggccaggt cggccaggtc cagtccctcg cccggctcga ccagcagcgt cacctcctgc
     2941 ttgccggcca cggcgacgct gatctcctgg gcggcgtcgc cgcccttgac ccggcggctg
     3001 aacaccggct gcccatcgcc caggacgctg aatacgcagt cgcccttccc ggcggcagcg
     3061 gcgtccaggc cgatcgtcgc ggcgagcgtg tcccacttgc cctcggcggc gaacgtcagc
     3121 gacgagcggg catgcacgcc gaggcccttg tcgtaggtct tggtgcccag cgtcagcggc
     3181 ttggagagca cgttcttgtc gcgctgcgcc gggcggggca gcgtgaccag ggccgtctgc
     3241 tcctcggcga ggggcttcag gtccgagagg tacgccacgc ggctgctgct gatcgtaatg
     3301 cgggccacca aagcccaggg cagcgcggtc ttcccgccgc ctgcgaaaga gagggtcgcc
     3361 gagccgccgg ccagttcgat cgagtacgcg cccagcagtg agccgccctg cagcgacagc
     3421 aagcagcggg ccggcggatc agccgccgcg ggctgcgcga cgacgacccc aaacagccgg
     3481 ctgcgcggga cgctgcgcga ctggccgccg acctcgaagg tcaattgctc ttcggtcagc
     3541 ttgtcgatca ggccggtaac gctgctcagc ttgcccagct cgtctttgag gaaaatgcgg
     3601 tcgagctccg ccgagggcgc cgccaccgcc ttttcgaagt cggggctggc cgccgcgggg
     3661 tcgaagcgaa tcgagcggac caggtcgatg gccagcgcca gcggggtttc gccgctccaa
     3721 gccacctggc acttgtcgtc gccgagcgtc acgcccttgg ctagcagctt gccgccgccg
     3781 cggagctcaa cgatcatggc cggcttttcg gtcaccgccg aaacgggcgc gacttcaatc
     3841 cgccgcagat cgtcgagcgt caggtcggcg ggcacgccct ctcccgaaag cttgccggca
     3901 gcgatttgca gcgactgaag cgggaccgcc gagccgtcga gcaggacgag ctgcggcgcg
     3961 gtcggcttgg ccggaacctg agcctgtgcg aagccaagca gtgcgaataa cgcagccagg
     4021 cagaggtggg gcgacgtcgg tctcatgcct ctatcttagt cggtgggttg gtgagtcggt
     4081 taatcggtaa gtcggatgcc taccgatcaa tcgaccgacc gacaaaccgg ccgacctctt
     4141 cgtcggaacg ccacagaggg cgttccctac ccctctgaat cgtcctggaa gctcttgtag
     4201 tactgctcga tgagctgctg gtagcgggcc gggaactttt ccttgatggc gctatagacg
     4261 gggtcgcggt ccttgtcgcg gagctggctc caggggctct gcgggccgcc gcggtgcagc
     4321 cgcttcacgg tgtcgctgtc gatccccttg ctgccgccgc cggagttgcc gctctggctg
     4381 ccctggccct gagcctggcc gtccccttcg ccgggcttgc tgccgggctt ctggccctgg
     4441 cctttgccgc cgcccttgca gttgcactcg cgctcttcgg cttccttgat cagcttggcg
     4501 aggatttcga tgatcttgtc ctgctgctgg cgcgtctcgg ttccagtgtc ttccagcgag
     4561 agtttgcggc ggctgaactc catccgcaag tggacgtcgc tcagcgtgcc ttcctggaag
     4621 agcttcaact gctccagctg acggaatgcg ccgacccgca tccgctcggg agcgtcgggg
     4681 taggtggcca ggaatttctc cagcgtggcc atcgcctctt tgtgccgcag cagggcgact
     4741 tccgccacgc cgcggaggaa gagcacttcc gcgccggtcg cggtcttgtc ggcccacttg
     4801 ccttccaggt cgcttagcaa cggcagcgcg tcttcaaatc gctcgtcgag caagtaggcc
     4861 cggccgagat agtagctggc gtcggcggcg agatacggat cagcattctc gcggagcttc
     4921 gtcaacccga cgatcgccgc gccgaggttg tcttcgccca gggcggtcag cgccgacctg
     4981 tagtccggat aaaggatgcg gagcgattcg gtgatcgcgg ccgcccggtc ttccggagtc
     5041 tggcggagct gcttcaccag ttccgcgacg acccgcttct gctcggcggt cgcgccgctc
     5101 ttctcgaccg aagcggtgaa gtcgtcgagc accttgttcg cgtccgcggc caacgcaccg
     5161 gcagtcaggc agcagaagca cacaatcgcg gaaaaccagc ggcgtgccat ggcggctcct
     5221 ccgggtggaa tggtcgatta agtatggcgg atcggcgggc ggttgtcgag aaagtttgcc
     5281 atccgcgacc actagccgcg gctttggtta cggtccccgc tccaggatgc ggatcgtcat
     5341 ttcgccgatt tccgcctggc gctgggcgat tttctgggtt tcgcctttca ggacgtcgtc
     5401 gagcggtgcg ttcttcgggc gggcctgctc cagcgaagtc gtgcggcggt tgacgcggag
     5461 ctgcgcggcg cggagcagct tcagctcggc cgaattcggc aatagcggtg gctccccgcc
     5521 accgcctcca ccgccaccca tgctttcttt ctgctgctgg gcctgctgca gggcttcgat
     5581 cagctcctcc agcgtcgttt cgatttcctt ctgcatcgag gcggtatagg tgtcggtccg
     5641 catgttgtcg gccaagaggc cgccgacggt aatcaggtcg tcgcggagtt gcccgacgac
     5701 atccgggaag acgacgctcg taccgtcgtc gaggatgatc tccagcgcct gctgggcctt
     5761 gccggccagg ccggcgtctt tggcttcggt ctcgctgcgg accgcttcca ttcgccgctc
     5821 gtcgtcgccg atggcccgga cggagttgcg atcgctgcgg gccagcgtgc cgccgttttc
     5881 gccgcgcttc ttttcgaggg ccgcggtctg taccgtcaaa cgctgctgaa tcgagagcat
     5941 ctcgcggaag cgggcctcca ggcgagcgag cttctcgagc tgcgtctctt cgcgaagctg
     6001 cgccagccgc tcttcaattt cacggagggc ttcttccagc tctttgatcg ccttgccctg
     6061 ctggcgattg gcgtcggccg gttcctgttt gcgcaggccg cccgaggctt gctgcatcga
     6121 cttctgggcc tgctgcacct tctgctggcc gggctgcggc ttgccactgc cgcctccacc
     6181 gccacctccg ccgccgcctt gggcggcggc cttggcagcg gcttcctgca tcttctgctc
     6241 gacctgggcg gtctgattgg cgatgtcgtc ctgcttcttg gccagtttct caaacgcttc
     6301 cggcggcagc gaagcaattc gctgggcctc gttcttcagc tcgttcaggg cccgctgcaa
     6361 attggccagt gcgttctctt catcctgctg ggcgttcttg cctttgcctt cctggaggtt
     6421 cttttcggcc tgcttctgat tctcgatcgc ctggccgagc ggcttttcgc cgggttcggc
     6481 gggacggttc ggcggggcgg ggggcact
//
LOCUS       DASRJB010000787         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3335399, whole genome shotgun sequence.
ACCESSION   DASRJB010000787 DASRJB010000000
VERSION     DASRJB010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335399"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFB80_20780"
     CDS             complement(<1..401)
                     /locus_tag="VFB80_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36282.1"
                     /translation="MRISITFIMAAAALALGGRSARAGDADVAPPVSQRFAPADVSEE
                     PSFQKHVSPLFGRLGCNGRACHGSFQGRGGFRLSLFGYDFKADHDELLKGDPQRANKD
                     KPLESLILVKPTDADMHEGGLRYKQGGWEYHL"
     gene            complement(470..1171)
                     /locus_tag="VFB80_20785"
     CDS             complement(470..1171)
                     /locus_tag="VFB80_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36283.1"
                     /translation="MQPSRASLALVGALALAGWLLAQDTGPQTKPPAGGRNVAGKGKA
                     NAKLPVTVTPEREAAVLTFIQRNHAELADLLAHLKENQPQAYEQAVRELFRTTERLAQ
                     IQERDPLQYELEVAVWKAQSRVQLVSARMKMAVTEDLKKELREALGVQSDARLALLKH
                     QRQQTSDRLTKLDADIARFENEREKVIDRQLQLLTRTAEGRTVEARGGKTGTKAAANK
                     AGGKKPASPKNVTPD"
     gene            complement(1172..1771)
                     /locus_tag="VFB80_20790"
     CDS             complement(1172..1771)
                     /locus_tag="VFB80_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36284.1"
                     /translation="MKHNEIQSELDWTAFCYAAGELTPVEVEQFEARLADEQEAREAL
                     ARAVELTQAVAAGESQVGSYAIAPAARQRSNWATRLAWMAVGGTAAALAAAIWSGAFH
                     RGQMPLTGDQLLARSELAAAWSQARDEIAAAKEAGQWPMIVSAADTDEEPLAAESRGD
                     DSALEDTPSWMTAAVFGLAGVSPADLEGALPFNNERGEN"
     gene            complement(1812..2369)
                     /locus_tag="VFB80_20795"
     CDS             complement(1812..2369)
                     /locus_tag="VFB80_20795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36285.1"
                     /translation="MSAEDRQNEAAGPLDPSVVAALFVEHGEELRRFLLGVLRDGQLA
                     ADVLQATFVKLVQRGHETRQETRKAWLFRVAYHEAMAIRRREGVGDKVLRNVAWHSNG
                     SAGPADEPVIRFESVAAVREALEQLPPDQRQVVRMRIYEEKTFAVIAKELKIPLGTAL
                     GRMRSALIKLRAKFNVKPDTGETEK"
BASE COUNT          369 a          834 c          786 g          483 t
ORIGIN      
        1 aggtgatact cccagccgcc ctgcttgtag cgcaggccgc cttcgtgcat gtcggcgtcg
       61 gtcggcttga cgaggatcag gctctccagc ggcttgtcct tgttggcccg ctgcggatcg
      121 cccttcagca gctcatcgtg atcggccttg aagtcgtagc cgaacagcga caggcggaag
      181 ccgccgcggc cctgaaagct cccgtggcag gcccggccgt tgcagcccag gcggccgaac
      241 agcgggctga cgtgcttctg aaagctcggc tcttcgctca catcggccgg agcgaaccgc
      301 tggctcaccg gcggggccac atcggcgtca ccagctcggg ctgaccggcc gcccaaggcc
      361 agcgccgcgg cggccataat gaaagtgata cttatccgca taaatcgtcc tccgcgattt
      421 tgtgtctatt cgggtatgtc gttttgaggt gggtcgttac gggcgggaat caatccggcg
      481 tcacgttctt aggcgaggcc ggcttcttgc cgccggcttt gttcgcggca gctttcgtgc
      541 cagttttgcc gccgcgggct tcgacagtgc ggccctcggc cgtgcgggtg agcagctgca
      601 attgccggtc gatgactttc tcgcgctcgt tctcgaagcg ggcgatgtcg gcatcgagct
      661 tcgtcaggcg atcggaggtt tgttgccgct ggtgcttgag cagggccagc cgggcgtcgg
      721 actgaacgcc caaggcctcg cgcagctcct tcttcaggtc ctcggtcacg gccatcttca
      781 tccgggcgct gaccagctgc acacgcgact gggccttcca gaccgcaacc tccagctcgt
      841 attgcagcgg gtcgcgttct tgaatttgcg ccagccgctc ggtcgtgcgg aacagctcgc
      901 gaaccgcctg ctcataggct tgcggctgat tttccttcag gtgagccagc agatcggcca
      961 gttcggcgtg gttccgctgg atgaatgtca gaacggccgc ttcccgctcg ggcgttaccg
     1021 tgacgggcag cttggcgttg gccttgcctt tgccagcgac attgcggccg ccagcgggcg
     1081 gcttcgtctg cgggccggtg tcttgagcca gcagccagcc ggccaatgcc agcgcgccaa
     1141 ccagagcaag cgatgcacgc gacggctgca tctagttctc tcctcgctcg ttgttgaacg
     1201 gaagcgcccc ttcgaggtcc gccggcgaga ctcccgccag gccgaacacc gcggcggtca
     1261 tccacgacgg cgtgtcttcc agcgccgagt cgtcgccacg cgactcggcg gccagcggct
     1321 cttcatcggt atccgcggcc gacacgatca tgggccactg gcccgcttct ttggcggcag
     1381 cgatttcatc gcgagcctgc gaccaggcag cggccaattc gcttctcgcc aggagctgat
     1441 cgccggtaag gggcatttgg cctcgatgga aagcgccgct ccagatggcg gcggccaacg
     1501 ccgcagccgt tccgccgacg gccatccagg ccaggcgggt ggcccaattc gatcgctggc
     1561 gggcggcggg cgcgatcgcg taactgccca cctgcgactc gccagcggcg acagcctgcg
     1621 tcaactccac ggcacgggcc aatgcctcgc gggcctcctg ctcgtcggcc aggcgggcct
     1681 cgaactgctc gacctcgacg ggcgttagct cgccggcggc gtagcagaac gccgtccagt
     1741 cgagctcgct ttgaatctcg ttgtgcttca ttgcagcgct tattggttgg cgtcgcctgt
     1801 gtcgggcttc gctatttttc tgtttcgccc gtgtcgggct tcacgttgaa cttcgctcgc
     1861 agcttgatca gcgccgaccg catgcggccc agcgcggttc ctagcggtat cttcaactcc
     1921 ttggcaatga ccgcaaaggt cttttcctcg taaatccgca tccgcacaac ctgccgctgg
     1981 tcgggaggca actgttccaa ggcctcacga actgcggcga ccgactcgaa ccggatcacc
     2041 ggctcatcgg cggggccggc cgacccgttg gagtgccatg caacgttgcg gagcactttg
     2101 tcgcccaccc cttcgcggcg gcggatggcc atcgcttcgt ggtaggccac gcggaagagc
     2161 caggcctttc gcgtttcctg ccgggtctcg tgcccccgct ggaccagctt cacgaacgtc
     2221 gcctggagca cgtcggctgc aagctgaccg tctcgcaaaa cccccagcag gaaccgccgc
     2281 agctcctcgc cgtgctctac gaacaaggcc gccaccacgg aggggtccaa aggccccgct
     2341 gcttcgttct ggcgatcttc cgcggacaca caagctcctg gccgaccgct cggctccgga
     2401 cgctcacagt tcagatgcgc tgtggcgggg ggatatttgg tcggaatctt gaaaaaatgg
     2461 gattcggccg gg
//
LOCUS       DASRJB010000788         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3607073, whole genome shotgun sequence.
ACCESSION   DASRJB010000788 DASRJB010000000
VERSION     DASRJB010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3607073"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFB80_20800"
     CDS             <1..628
                     /locus_tag="VFB80_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36286.1"
                     /translation="QARGDAVDSASDLFSLGSVLYAMCTGRPPFRAETAYGVLRRITD
                     SEARPVRETNPQTPAWLDALVQRLLSKSPGERIGTADELADVLAQCLAHVQRPTATPL
                     PTVVSKLAPASKPAASRNWLWVGVPALALAAAGILLQANWPKQPSGGSTVLTGPAAPA
                     DPATDWDDGSSEQLQSLRRELDQLEPRARRLWDTNPSIDSAFSKENQP"
     gene            625..3279
                     /locus_tag="VFB80_20805"
     CDS             625..3279
                     /locus_tag="VFB80_20805"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN36287.1"
                     /translation="MKTFLSFLALLSLAAAAAWAQTPPPDSPKISVTTKPPNALQPEG
                     EQNVVFQLRHMQANEMLVMLQPLFPRNAVMSADTGRQRIVVSSTPAVMQKIEELIKQL
                     DTPPPPAGTTADPTRRPPRAATPSDSTLGPPTTPEGHQIEAISLRHISVESGLPIIRQ
                     LLPPDARIIGDQEQQRVIVTGSQADIDAVKKTIILLDTPAPQRASTPGPAGRAATAQN
                     QAQTTASAFLRQLQQQAQLPQFPQPAAAASDLARRYQELEQEAQSLAQIWRTLTAKPQ
                     GDVQTSAEIATTKTRLQSVVAQAFAARQKQQQEELLEMQRRIYSLAGTIEDREKLKDQ
                     IIERRVQDLLNPALQWTPPGAAAAARAGQPPAGATGTPGYSTPRYPTLGAPTGRTAPA
                     ADSDSGAIMQIADEMVHVSLGKDAGITSGQALYVYRADECVAELEVIAVQAKHAVTRI
                     KLKTGALRPSDRVSTKAPAPAAVDPKPLPTQSDAGRPLSDAVREFNALNADKPLNRDQ
                     PPLTDDEVVAAIRWTTQDRKSLLASDEEIQALLAVADHRVLPPKAQLRMGGETVTPGK
                     GQASKVVICLELESARGPAYRHVLREQYIGYQGPFRPRDKSEASQLSTAIKAFNQRHL
                     NDPIGKNQPLLSEAEVIAAIVGWASKRDDLPVTNAEFDELQTIAVTGTLPPEAELEVL
                     TSFQPNDELQFDAWSVRLRLQRGEGGGSYAYTIRNRWLRVKALKERKISWGPAAANGL
                     QVGVWIDPPGSVFAKGQQFVPHFYFRNAGQKDQSISLPRIMTRSYYEGLLVADDSGAA
                     IEYDQDKGPSGPVGWIQMPFAPGAEHEVMGMPIAVGQVKRAPGVETVLCLTSGQQCRL
                     KFLLDNYLDRESKEPLGTGEIRFSLADE"
     gene            complement(3283..>4121)
                     /locus_tag="VFB80_20810"
     CDS             complement(3283..>4121)
                     /locus_tag="VFB80_20810"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN36288.1"
                     /translation="IFPELKAGGPESAAAVPLPQDLPAAEEPGDEDEFDEDDPNDEEQ
                     DDDDLGDATFAVEAPVLKEKRVKVGEKVCAIGIYSQPRGGLTPGGMGLDKFIKLVRGN
                     IDIVEKKARGAVFGNLIGGIVVLILVNAATYGVLMANRHHPAAVSERQRDAVQAISKD
                     DAARLDKLLARGMDVNALESGRTLLHNTDDAACARVLLARGANLEARSDEGLTPLMEA
                     ARRGHLAVAQVLLEAKVDVNAINKEHPMTALDYAVRAGQADVEALLRQAGGKPAPMAE
                     QP"
BASE COUNT          748 a         1399 c         1323 g          651 t
ORIGIN      
        1 gcaggcccgc ggcgacgcgg tcgatagcgc cagcgaccta ttcagcctgg gcagcgtgct
       61 ctatgccatg tgcaccggcc gcccgccctt ccgggctgaa acggcctacg gcgtcctgcg
      121 gcggatcacc gacagcgaag cccgcccggt tcgcgagacg aatccgcaaa cgcccgcctg
      181 gctcgatgcg ctcgtccagc ggctgctcag caagtcgccc ggcgagcgga tcggcaccgc
      241 cgacgaactg gccgacgtgc tcgcccagtg cctggcccac gtgcaacggc caacggccac
      301 gccactgccg accgtcgtga gcaagctcgc gccggcctcc aagccggcag cctcgcgcaa
      361 ctggctctgg gtgggcgttc ctgctctggc cctggcggct gcgggaatcc tgctgcaggc
      421 caactggccg aagcaaccat ccggcgggtc gaccgttttg acaggtcccg ccgcgccggc
      481 cgacccggca accgactggg acgacggctc gtcagagcaa cttcagtcgc tccgccgcga
      541 gctcgaccag ctcgagccgc gggcccggag gctgtgggat acgaatccat cgatcgactc
      601 tgccttttcc aaggaaaacc agccatgaaa acgtttctct cgttcctggc gctcctgtcg
      661 ctcgcggctg ccgcggcttg ggctcagacc ccgccgccgg acagccccaa gatatccgtc
      721 acaaccaaac caccgaatgc actgcagccc gaaggggagc agaacgtcgt gtttcagctc
      781 cgccacatgc aggcgaacga aatgctggta atgctccagc cgttgtttcc gcgcaatgcg
      841 gtcatgtccg ccgacaccgg gcggcagaga atcgtcgtct ccagcacgcc cgccgtgatg
      901 cagaagatcg aggagctgat caagcaactc gatacgccgc ctccgcccgc cggtacgacc
      961 gccgatccga cgcgccggcc gccgagggca gcaacgccga gcgactccac gctcggcccg
     1021 ccgacgacgc cggaaggaca tcagatcgag gcgatttcgc tccggcacat cagcgtggag
     1081 tcggggctgc cgatcatcag gcaattgctg ccgccggatg cccggatcat cggcgatcag
     1141 gagcagcagc gggtgatcgt gacgggctcg caggcggaca tcgacgcggt gaaaaagacc
     1201 atcatcctgc tcgacacgcc agcgccgcag cgggccagca cgccgggtcc ggcaggccgt
     1261 gccgcgaccg ctcagaatca ggcacagacg acggccagcg cgttccttcg gcaattgcaa
     1321 caacaggctc agcttccgca atttccgcaa ccggccgccg cggcgagcga cctcgcgcgg
     1381 agatatcagg agttggagca ggaggctcaa tcgttggctc agatctggcg gacgcttacg
     1441 gcgaagcctc aaggcgatgt gcagacttcg gcggagatcg caacgaccaa gaccaggctc
     1501 caaagcgtcg tagcgcaggc ctttgccgcg cggcaaaagc agcagcagga ggaactgctg
     1561 gaaatgcagc ggcggattta cagcctggcc ggcaccatcg aggaccgcga gaagctcaag
     1621 gaccagatca tcgagcgccg cgtgcaggac ttgctgaacc cggcgctgca gtggacgccg
     1681 cccggggccg cagccgcggc tcgcgcagga cagccacctg ccggcgcgac cggcacgccc
     1741 ggctattcca cgcccaggta tcccacgctc ggtgcgccaa ccggccggac cgctccggcc
     1801 gccgactccg acagcggggc gatcatgcaa atcgccgacg agatggttca cgtttcgctt
     1861 ggcaaagacg caggcatcac gagcggccag gccttgtacg tttaccgagc ggatgaatgc
     1921 gtcgcggagt tggaagtcat cgccgtgcag gcgaaacatg ccgtcacccg gatcaaacta
     1981 aagaccggcg ccctgcgccc gagcgaccga gtctcaacga aggcgcccgc tccagcggcc
     2041 gtggatccga agccgctgcc gactcaatcg gacgcaggcc gaccgctttc cgacgccgtc
     2101 cgcgagttca atgcgctgaa cgcggataag ccgctcaatc gcgatcagcc gccgctcacg
     2161 gacgatgaag tcgtggcggc cattcgctgg acgacgcagg accggaaatc gctcttggct
     2221 tcagacgagg aaatccaggc gctgttggca gttgccgacc accgggtcct gccgcccaag
     2281 gcccagcttc gcatgggggg tgagactgta acacccggga agggtcaggc ttcgaaggtc
     2341 gtgatttgcc tcgagctgga aagcgcgcgt ggcccggcgt atcgacacgt gctgcgcgag
     2401 cagtatatcg gttatcaggg gccattcagg ccgcgcgaca agtcggaagc cagccaactg
     2461 agtaccgcca tcaaggcttt caatcagcgc catctcaatg atcctattgg caagaatcag
     2521 ccgctactaa gcgaagcgga ggtgatcgca gcgatcgtgg gctgggcaag caaacgcgac
     2581 gacctccccg ttaccaatgc cgagtttgac gagctgcaaa cgattgccgt gacgggaacg
     2641 cttccgccgg aggcggaact cgaagtcctg accagctttc agcccaacga cgagctccag
     2701 ttcgacgcct ggagcgtgcg gctgcggctg cagcgaggcg agggaggcgg cagctacgcc
     2761 tacacgattc gcaaccgctg gctccgcgtg aaagcgctga aggagcggaa gatttcctgg
     2821 gggccggcgg cggccaatgg cctgcaagtc ggcgtctgga tcgatcctcc gggcagcgtc
     2881 tttgccaagg gccagcaatt cgtgccgcat ttctatttcc gcaacgcggg tcagaaggac
     2941 cagtcgatca gcctgccgcg gatcatgacg cgcagctatt acgaggggct cttggtcgcc
     3001 gacgattcgg gcgcggcgat tgagtacgac caggacaaag gtcccagcgg gcccgtcggc
     3061 tggatccaga tgccgttcgc ccccggcgcc gaacacgaag tgatgggaat gccgatcgcc
     3121 gtgggtcagg tcaagcgagc gccgggcgtc gaaacggtgc tttgcctgac atccggccag
     3181 caatgccgcc tgaagtttct gctcgacaac tatctcgatc gcgaatccaa ggagccgctc
     3241 ggcacgggcg agattcgctt ttcgctggcg gacgagtaac tgctagggct gttcggccat
     3301 cggagccggt ttgccgccgg cctggcggag cagcgcctcc acatcggcct ggccggcgcg
     3361 gacggcgtag tcgagcgccg tcatcggatg ctccttgttg atggcgttga cgtcgacctt
     3421 ggcctccagc aggacttgag ccacggccag gtggccgcgg cgggcggctt ccatcagcgg
     3481 cgtcagccct tcgtccgatc gagcctcgag attcgccccg cgggccagca gcacgcgggc
     3541 gcaagctgcg tcgtcggtgt tgtgcagcag cgtgcggccc gattccagcg cgttgacgtc
     3601 catcccgcgc gcgaggagct tatccagccg cgccgcgtcg tccttgctga tcgcctggac
     3661 tgcgtcccgc tgccgctcgc tgaccgccgc cgggtgatgc cggttggcca tcagcacgcc
     3721 gtaggttgcg gcgttcacca ggatcagcac gacgatgccg ccgatcagat tgccgaagac
     3781 cgcgccgcgg gctttcttct ccacgatgtc gatgttgccg cggaccagct tgatgaactt
     3841 gtcgagtccc atgccgcccg gagtcaaccc gccccgcggc tggctgtaaa tgccgatcgc
     3901 acagaccttc tcgccgacct tgacgcgctt ctccttgagc acgggcgcct ctaccgcgaa
     3961 tgtcgcgtcg cccaagtcgt cgtcatcctg ctcttcgtcg ttgggatcgt cctcgtcgaa
     4021 ctcgtcttcg tcgcctggtt cttccgcggc cggcaaatcc tgcggcagcg gcactgcggc
     4081 tgccgactcc gggccgccgg ccttcagctc cgggaagatg t
//
LOCUS       DASRJB010000789        13129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3335799, whole genome shotgun sequence.
ACCESSION   DASRJB010000789 DASRJB010000000
VERSION     DASRJB010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335799"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFB80_20815"
     CDS             complement(<1..499)
                     /locus_tag="VFB80_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36289.1"
                     /translation="MSQAFDAYQAWLGIPPAEQPPNHYRLLGVRLFEANLEAISISAE
                     QVRARLAAFRHGPEQLVADRLIADVTAAREVLLNAAQKAHYDGILRAQLAARPADTWP
                     PGMTRPAAIAQAAAPAPRPSDSRPLQPVVVPNAQAIVDPLENMPQATPVNSQRPLRVA
                     QPLAVA"
     gene            complement(583..2109)
                     /locus_tag="VFB80_20820"
     CDS             complement(583..2109)
                     /locus_tag="VFB80_20820"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN36290.1"
                     /translation="MPDAPIPADVIPPGDLPPLRERDLPPPIPLRQMIGPSIILAGLA
                     LGSGEYVLWPYITFQSRFVFFWACLLAVVMQYFLNLEIMRWTLATGESAMTGIIRLSR
                     HLAGVFLVLNIVPWMLPAWATGAAQLVGWLIQEPALDAAGNIVPGPYDTWLALAGILV
                     CGVILTAGPVVYETVERVQFVLVMLIIVLVVGLAAWLIVGRPDALLAQARSTLTLGYP
                     KFLPPLGAELTPIVLLGALAFAGAGGTMNLCQSNYIRDKGYAMGSHVGRITSPITGKP
                     EPITEVGYHFPHTAENLRRWAAWWRGACWEHFYSFLLTCLVCLVLLTLISYICFYDAS
                     GQRLPGAERYKGMAFVWGEAQRLVTLIGPLARWLFLIMGVAILFTTEFGVLDAASRIS
                     TDIVKVAWLRDNPRWSEGRLYYLFLWAEIALACTILLLDHYQIKRVGAFALFEFSSAM
                     NGGVMFLYSLALVVMNRWRLPAAIRISSWRLAFLLLTAAFFGFFSVWAAYEAVMKLAG
                     "
     gene            complement(2114..3427)
                     /locus_tag="VFB80_20825"
     CDS             complement(2114..3427)
                     /locus_tag="VFB80_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36291.1"
                     /translation="MAIAALLVAVVPLTAALSPAQLPKAPARPSPHGRGSDELAPRVD
                     EGKVAAAGIRRLAGRHVTLYTDLPAAAELDDMPAVFDAAVPLWADYFAVPAGKLSGWK
                     LVGCVMQNKDRFAGAGLFPDNLPDFQHGYSQDSQFWLYEQPSAYYRRHLMLHEGTHCF
                     MQRWLAGAGPPWYMEGIAELLGTHRWAGGKLTLGVMPATKEELPYWGRVKILKEELAA
                     GKALTLPQIMQYDSRAHLRQEPYGWCWAAAAFLDGHPLTQAPFRELKSQTADRSSAFS
                     QRLAERLKPQWPAISQDWTLFVQECDYGYDFARAAVVHKAAQPIAPAGAAMAVDAARG
                     WQAAGVALEAGKRYAISASGRFEVARQPRPWPCEAGGVTIRYHAGRPLGMLLASVDLD
                     EFTPIGTSAALTPKHSGPLYFKINEASGGLADNSGSLTVVVKPLP"
     gene            3522..4118
                     /locus_tag="VFB80_20830"
     CDS             3522..4118
                     /locus_tag="VFB80_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36292.1"
                     /translation="MSDSPNEALPRRGPPAHWIVAILALAGAGAVQVNWGAALGGPAA
                     AGLWPYSIAALVAATAWLLSTAWLHWRLVARRPAELHGHLRRLMTLALSELLVASFAA
                     LLLWQGHGSRVLLAGLWTVPPVLLVVAAISSAALLAAAGSGAREVSAIAPNSLEAAPP
                     SDRWRTVIVLALLISLGLLALRPLPEPVHSSRFEAKQL"
     gene            complement(4141..5916)
                     /locus_tag="VFB80_20835"
     CDS             complement(4141..5916)
                     /locus_tag="VFB80_20835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36293.1"
                     /translation="MRCNILAALLTGFALVLALAGRGSAQGAAGAMVRLLESGRVPPE
                     RQATIIEMICSRGEAGDLAVIFKLLAKPDGMPPALKRKVVELLTEAALSRQVKPSGDL
                     SALTKLIDGEAAAQDRRMQLAAIKLAAAWKLKEAAATLERLATDGNAQESLQQAAIEG
                     LVAVGGAESRLTIEKLAAGGKSTRVRIAAAAALAKLDAGAAAKAAADVLAKVGPQDDV
                     GPLLDSLLSRNDGAERLAEALATQPPPADAAKIALRYMYSVGRSDPGLSDVLSKAAGI
                     ALDAPPPPPEEAAKIAAQVAAKGDAARGEKVFRRADLSCMKCHAVSQAGGTVGPDLSS
                     VGSISPVDYIVNSILNPTLAVKEQFVTRRILTLDGEVITGIQVDRDEQKLRLRDAAGK
                     VVTIPVASIDQEGEGKSLMPQGLTKFLTQQEFLDLAKFISELGKPGPYAIRKTPSIQR
                     WRVLKNPGPELTGEVPNVEILREQVLDAAPEAWTTAYGTVGGELPLAELAPQRPAVVY
                     LQGEIEVSQAGAIACEVSSDQGLQVWLDAEPFESTRRIERALAAGKHTLTFRVQVGSQ
                     PEPTLKVELTKPAGSTAQFVVINGM"
     gene            complement(6145..6489)
                     /locus_tag="VFB80_20840"
     CDS             complement(6145..6489)
                     /locus_tag="VFB80_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36294.1"
                     /translation="MAYDIVITEDAERQLRGFPARQCRFVEDAILARLVHEPTKISQA
                     IKRLRPNPFAEYELRVSDIRVLYNVEDDKVVLLLIGRKVGNKLVVEGQEFHGHKDDPP
                     QPPGSGSSPDAE"
     gene            complement(6540..9248)
                     /locus_tag="VFB80_20845"
     CDS             complement(6540..9248)
                     /locus_tag="VFB80_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN36295.1"
                     /translation="MSRTISYLVVLALLLIGAPASAVDEPRLQLAKGQHVALIGNTLA
                     ERMQYFGNFETNLHARFPELELIVRNLGWSADEVALRPRSQAFQDHGHTLFDHKADVI
                     LAFFGFNESFGGEQGLPKFEADLDKFVAEILATNYNGTSPPQLALISPIAHENIHRRG
                     YPDGTANNKNIALYTAVMKKVAAKHKVPFVDLFQPSQELFIGSKEALTINGIHLAENG
                     DAFIAEILDKALFGPRPAARVDLSKLRAAVNEKNQQFFYDHRAVNGFYIYGGRKNPFG
                     VVNFPVEFAKLRMMIDVRDRRIWDIAQGKKVPETIDDSSTGENARIETNYKNIVSINP
                     PEEAQQKFTLPEGYAVNLFASEREFPDLQKPVAFAFDAKGRLWVCTMPSYPQYLPGSP
                     PNDKVLILEDTNGDGRADKQTVFADKLYLPAGIEFWGGGIIIAQQPNVMFLKDTNGDD
                     QADVRELILHGFDSADSHHAISAFALDPGGALYFEEGTFHHSQIETPYGPERCANAGV
                     FRYEPRTGKLDVFVSYGFANPWGHIFDSWGQNFVADASPGFNYFGAAFSGDVDYPHKH
                     GALKTFLTKQWRPTCGCDLVTSRNFPPEAQGNYLLNNCIGFQGVLQYKMKDEGSGFAA
                     DPVDPLLQSSDPSFRPVDIEFAPDGSLFVADWYNPLIGHMQHSIRDPNRDHAHGRIWR
                     VHYTKLPLVMPPKIAGQPIAVLLDLLKTEPEARTHERVRSELWNRPTDQVIGEAGRWL
                     AALPKNDPLYEHHKLEALWLHQAHDAINEPLLKEVLRSPDYRARAAATRVLGYWRDRV
                     EKPLDLLRAQVADEHPRVRLEAVRALSFFNLQAAIDVAVEALLYDQDDYLKYTFNETM
                     KTLERRVKGATSLPHEGTEWDKRNAEGYKVPPEGAKGKAKANNGKE"
     gene            complement(9323..9994)
                     /locus_tag="VFB80_20850"
     CDS             complement(9323..9994)
                     /locus_tag="VFB80_20850"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN36296.1"
                     /translation="MPADVVAAVSPRELLKDRAYEALKERILSSEFPPGSFLAERQLA
                     EQLGMSKTPVKAALERLQFEGLIAISPQQGIVVRDLAIQEIADQYEIRVALETFILRS
                     LAGKLTPEQIARVRGNLEAQRQIQGTGDVARGVELDAEFHMQFAEFFSNREILRVMRQ
                     LRDKVQRVITRVFRLNPGRIDTSFGEHLAIAEATIAGDGASAAQLLEAHLDRGRRLIL
                     SPRAA"
     gene            10111..11604
                     /locus_tag="VFB80_20855"
     CDS             10111..11604
                     /locus_tag="VFB80_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HZN36297.1"
                     /translation="MYRPIALRLSLVSLLLLLIHCAARSWADEPAPWAKAAVPAFPGA
                     EGAGAWTPGGRGGQVLVVSSLEDSGPGTLREALATRGPRIIVFGVAGIITLASPLEIS
                     EPFVTLAGQTAPGDGVCIRGESTAINTHDVVIRYLRFRRGNLQRRDDALGGNPAGNVI
                     IDHVSASWGLDENLSLYRHMSPLPGGGQRKGPVENLTIQWSISSEALNLNNHAFGGTW
                     GGKNSSFHHNLFACNTGRNPSIGMGGGFDYRNNVIFNWIHRTMDGGDGSSRVNVIANY
                     YKPGPATEGELRYRICKAQARNSRDQFPGFGKWFVEGNVVEGNAAVTADNWAGGVQYD
                     AAHRDRDEIIPAGTEAAVRSREAFPAASVTTHSAQEAYELVLAHAGASLPRRDPVDER
                     VLESVRTGKPAFGVGILQTPADVGGWPEYKAAETPRDGDRDGLPDIWEKSHGLNPSDA
                     ADSALDGDRDGYTNIEEFLNGTDPRELVDYTRPENNRSALQAAPRSR"
     gene            11601..>13129
                     /locus_tag="VFB80_20860"
     CDS             11601..>13129
                     /locus_tag="VFB80_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36298.1"
                     /translation="MIRRLLASSILATTAAMQLAAADPSARFVYPDRAGKLVYDADAR
                     GNRIPDFSHCGYGGGGAAIPNVPTRVTVPPKVGDATSRIQAAIDYVSRLPADQQGLRG
                     AVQLLAGRHSIAGQLRIAASGVVLRGQGAEGGGTTLLATGTDRRTLIRVAGKSDRRIS
                     PAVHAVADEYVPVGACTLRLKSTDGLQVGNTVLVTHPSPREWIAAVGMNRFPSRDVGS
                     WLDWQPGKMDVRWDRVITKIDGEAITLDAPLTTALDAATGAGTVASYDWPGRIRQSGV
                     ENLRCESAFDPANPLDEEHAWGAIGLENVQDAWVRNVTAVHFAGSAVAVWESCKQITV
                     EDCTSLAPVSELGGYRRHTFFTSGQQTLFRRCRAEQGRHDFSVGYLAAGPNAFVECTA
                     DQAHQYSGPIESWASGVLYDNVVMDGGGLALTNRETAAQGAGWAAANCVLWQCTAPLV
                     TCRMPPTAQNWAIGCWGEFVGDGHWRSLNEFVEPKSLYAAQLAERMGAASARPPVPPA
                     NT"
BASE COUNT         2179 a         4310 c         4358 g         2282 t
ORIGIN      
        1 gagcaacggc cagcggctgg gccacgcgga gcgggcgctg ggaattcacc ggcgtcgctt
       61 gcggcatgtt ctccaagggg tcaacaatcg cctgcgcgtt gggcacgacc accggttgaa
      121 gcgggcgcga atcggacggc cgcggcgcgg gcgctgccgc ttgcgcgatc gcagcaggcc
      181 gggtcattcc cggcggccac gtgtcggccg gccgcgcggc aagttgggca cgcagaatgc
      241 cgtcgtagtg ggccttctgc gccgcgttga gcaggacctc ccgcgccgcc gtgacatcgg
      301 cgatcaggcg gtcggcgacc agttgctcgg gtccatggcg aaaagcggcc aggcgggccc
      361 gcacctgctc ggcggagatc gagatcgcct ccaggttcgc ctcaaagagc ctgacgccca
      421 gtaagcggta gtggttgggc ggctgctcgg ccggtggaat gcccaaccat gcctggtagg
      481 cgtcgaaagc ctggctcatg cgtccacctc aagaccccta tgaaagcgat ccgcgatttt
      541 acctcaggcg gctgatcggg gccagcaaga tgcttcggcc gctcagccgg caagcttcat
      601 cacggcttcg taggcggccc aaacggagaa aaagccgaaa aatgcggcgg tgagcagcag
      661 gaacgccagc cgccagctgg aaatgcggat cgccgccggc agccgccaac ggttcatgac
      721 gaccagggcc agcgaataga ggaacatcac gccgccgttc atggccgagg aaaactcgaa
      781 gagggcaaat gcgccgactc gtttgatttg atagtggtcg agcagcagga tggtgcaggc
      841 cagcgcgatc tcggcccaca ggaacaggta gtacagccgg ccttcgctcc accgcgggtt
      901 gtcgcgaagc caggcgacct tgacgatgtc ggtcgagatg cggctggcgg cgtcgagcac
      961 tccgaactcg gtggtgaaaa gaatggcgac gcccatgatc agaaacagcc acctggcgag
     1021 cgggccgatg agcgtgacca gccgctgggc ctcgccccag acgaaggcca tgcccttgta
     1081 gcgctcggcg ccgggcaggc gctggccgga tgcgtcgtag aagcagatgt agctgatcag
     1141 cgtgagcagc acgaggcaga ccaggcaggt gagcaggaag ctgtagaagt gctcccagca
     1201 ggccccgcgc caccaggcgg cccagcggcg gaggttctcg gccgtgtggg gaaagtgata
     1261 gccgacctcg gtgatcggct cgggcttgcc ggtgatcggg ctggtgatgc ggccgacgtg
     1321 gctgcccatc gcatagccct tgtcgcggat gtagttgctc tggcagaggt tcatcgtgcc
     1381 gcccgcgccg gcgaaggcca gcgccccgag gagcacgatc ggcgtcagct cggcccccag
     1441 cggcggcagg aattttggat acccgagcgt gagcgtcgag cgggcctgcg cgagtagcgc
     1501 gtcgggacgg ccgacgatca gccacgccgc gaggccgacg acgagcacga tgatcagcat
     1561 gacgagcacg aactggaccc gctcgacggt ttcatagacc acgggcccgg cggtgaggat
     1621 gacgccgcag acgaggatgc cggccagcgc cagccaggtg tcgtacgggc cgggcacgat
     1681 gttgccggcc gcgtcgagcg ccggctcctg gatcagccag cccacgagct gcgccgcgcc
     1741 ggtggcccag gcgggcaaca tccagggaac gatattcagc accaggaaga cccccgccag
     1801 gtgccgcgag aggcggatga tgccggtcat cgcgctttcg ccggtggcca gcgtccagcg
     1861 catgatctcc aggttcagga aatactgcat cacgaccgcc agcaggcagg cccagaagaa
     1921 gacgaaccgg ctctggaacg tgatgtacgg ccagagcacg tactcgccgc tccccagcgc
     1981 gaggccggcc aggatgatgc tcgggccgat catctgccgc agcggaatcg gcggcggcag
     2041 gtcgcgctcg cgcagcggcg gcaggtcgcc cggcgggatc acgtcggcgg gaatgggtgc
     2101 gtcgggcatg cggctacggc agcggcttga cgacgacagt cagcgacccg ctgttgtcgg
     2161 ccagcccgcc gctggcttcg ttgatcttga aatacagcgg cccgctgtgc ttgggcgtca
     2221 gcgccgcgct cgttccgatg ggcgtgaact cgtccagatc gaccgacgcc agcagcatcc
     2281 ccagcgggcg gcccgcatga tagcggatcg tcacgccgcc cgcctcgcag ggccacggcc
     2341 gcggctgccg ggccacctcg aatctcccgc tggcggagat tgcgtagcgt ttgcccgctt
     2401 ccagcgcgac gccggcggct tgccagcccc gcgcggcatc gacggccatc gccgcacccg
     2461 ccggcgcaat gggctgcgcg gccttgtgca cgacggccgc ccgggcgaag tcgtagccgt
     2521 agtcgcactc ctgcacgaac agcgtccaat cctggctgat cgccggccac tgtggcttga
     2581 gccgctcggc cagccgctgc gagaaggcgg acgagcggtc ggcggtctgc gacttcagct
     2641 cgcggaacgg ggcctgcgtg agcgggtggc cgtcgaggaa cgccgccgcg gcccagcacc
     2701 agccgtaggg ctcctgccgc aagtgcgccc ggctgtcgta ctgcatgatc tgcggcagcg
     2761 tgagggcctt gccggcggcc agctcctctt tgaggatctt cacgcggccc cagtacggca
     2821 gctcttcctt ggtcgcgggc atgacgccca gcgtcagctt gccgccggcc cagcggtgcg
     2881 tgccgagcaa ttcggcgatg ccctccatgt accagggcgg ccccgcgccg gcgagccagc
     2941 gctgcatgaa gcagtgcgtc ccttcgtgca gcatcaggtg ccgccggtaa tacgcgctgg
     3001 gctgctcgta gagccagaac tgcgagtctt gcgaatagcc gtgctggaaa tcggggaggt
     3061 tatccggaaa gaggcccgcg ccggcgaagc ggtccttgtt ctgcatcacg caaccgacca
     3121 atttccagcc cgagagcttg cctgccggca cggcgaagta gtcggcccac agcgggacgg
     3181 ctgcgtcgaa gacggccggc atatcgtcga gctcagcggc cgccggcagg tccgtataga
     3241 gggtgacgtg ccggcccgcg aggcggcgaa ttccggccgc cgcgactttg ccctcatcga
     3301 cccgcggtgc gagttcgtca ctccctctcc cgtgtggtga gggtctggct ggcgccttcg
     3361 gcaactgagc cggcgacagc gccgcagtga gcggtacaac agcgaccagc aacgcggcga
     3421 tggccaattt agttcttagg ttctttggtc tttcgctcat gaggcctcgc cggctggcgt
     3481 ctgctattct aaccgctcgc tgttttccgc ggatgcgatt catgtccgac tctcccaacg
     3541 aagcactgcc gcggcgcggg ccgccggcgc actggatcgt cgccatcctg gcgctggccg
     3601 gagccggggc ggtgcaggtg aactggggcg ccgcgctcgg tgggcccgcc gcggccggat
     3661 tatggcccta ttcaattgct gctctggtag ccgccacggc ctggctgctc tcgacggcct
     3721 ggctgcactg gcggcttgtc gccaggcggc cggcggaact gcacgggcac ctgagaaggc
     3781 tgatgacgct ggcgctttcg gagctgctcg tcgccagctt cgcggcgctc ttgctctggc
     3841 agggacacgg ctcgcgggtg ctcttggctg ggctgtggac cgtgccgccc gtgctcctgg
     3901 tggtggctgc gatctcgtcg gccgcgctgc tggccgcggc cggcagcgga gcgcgcgagg
     3961 tttcggcaat agcgcccaat tcgcttgagg cggctccgcc gagcgaccgc tggcggacgg
     4021 tgatcgtgct cgcgctgttg atctccctgg gcctcttggc cttgcggccg ttgccggagc
     4081 cagttcacag ttcaaggttt gaagccaaac aactatgaac tttgaacctg gaacgtgcgc
     4141 ctacatcccg ttgatgacga caaactgtgc tgttgagccg gcgggctttg tcaattcgac
     4201 tttgagcgtc ggctcgggct gcgagcccac ttggacgcgg aacgtcagcg tgtgcttccc
     4261 ggcggctaac gcgcgctcga tgcgcctggt ggactcgaag ggctcggcgt cgagccaaac
     4321 ttgcaggccc tgatcgctgg aaacttcaca agcgatcgcg ccggcctgac tgacttcgat
     4381 ctcgccctgc agataaacga ccgccggccg ctgcggagcg agctcggcca gcggcaactc
     4441 gccgccgact gttccatagg cggtggtcca cgcttcgggt gccgcatcga gcacctgctc
     4501 gcgaaggatt tcgacattcg gaacttcgcc ggtcagttcc ggcccgggat tcttcagcac
     4561 ccgccaccgc tggatgctgg gcgtcttacg aatggcgtag ggcccaggct tgccaagctc
     4621 ggagatgaac ttcgccaggt cgaggaactc ctgctgcgtg aggaatttgg tcagcccctg
     4681 cggcatcagc gacttgccct cgccttcctg gtcgatgctg gcgactggga tcgtgacgac
     4741 tttgcccgcc gcgtcgcgca ggcggagctt ctgctcgtcc cggtcgacct gaatgccggt
     4801 gatgacctcg ccatcgagcg tgaggatacg gcgggtgacg aactgctctt tcactgcgag
     4861 cgtcgggttg aggatcgagt tgacgatgta atcgacgggc gagatcgaac ccacggagct
     4921 gaggtcgggg ccgacagtcc cgcccgcctg ggagactgcg tggcatttca tgcagctcag
     4981 gtcggccctc cggaagactt tttcgccgcg agcggcgtcg cccttggccg cgacttgcgc
     5041 tgcgattttc gccgcttcct ctggcggcgg cggcggagca tccagcgcga tgcccgcggc
     5101 cttgctgagc acatcggaaa gccccgggtc gctgcggccg accgaataca tgtagcggag
     5161 ggcgatcttg gcggcgtccg ccggcggcgg ctgggtcgcg agcgcctctg ccagtcgttc
     5221 cgcaccatcg ttccggctca gcagcgaatc gagcagcggg ccgacgtcgt cctgcgggcc
     5281 gactttcgcc agcacgtctg ccgcggcctt cgccgccgcg ccggcgtcca atttggccag
     5341 cgccgctgcc gcggcgattc gcacgcgagt cgacttgccg ccggcggcca gtttctcgat
     5401 cgtcaagcgg ctctccgcgc cgcccaccgc gacgagccct tcaattgccg cttgttggag
     5461 gctctcctga gcgtttccgt ccgtagcgag gcgctcgagt gtcgccgccg cctccttcag
     5521 tttccacgcg gcggcgagct tgattgccgc cagctgcatc cgccggtctt gggcggctgc
     5581 ctcgccgtcg atcagcttcg tcagcgccga caggtcgccg ctcggtttca cctgccgcga
     5641 gagcgccgcc tcggtcagca gctccacgac cttgcgcttc aaagccggcg gcattccgtc
     5701 cggcttcgca agcagtttga agatgaccgc cagatcgccc gcctcgcccc gcgagcagat
     5761 catttcgatg atcgtcgcct gtcgctcggg cggcacccgc ccgctctcca gcagccgcac
     5821 catggccccg gcagcgccct gggcggagcc ccgacctgcc aaagccagaa ccagcgcgaa
     5881 gcccgtcaat agggccgcga ggatattgca gcgcatgaga gggcgatcgg caaaactgac
     5941 gacctcaacc attagccgtc cgtggagcgg acaaaaggcc ttcgggcgcc ggatttcgac
     6001 ttagccacta acgcttgaaa cgctgggtcg ctcgcaatca actcgtcgat cagcgactcg
     6061 ttgtccgtgg gcgagagagg ctgcagggcg accagtcgat ggtcgggcaa ctcgaccaaa
     6121 acgggcagtc ccgagtcggc gcagtcattc agcgtctggc gaggatccgc ttccaggcgg
     6181 ctgaggggga tcgtctttat gtccatggaa ttcctgaccc tcaacaacca gcttgttgcc
     6241 caccttgcgc ccgatcaaaa gcaagacaac tttatcatcc tcaacattat acaacacgcg
     6301 gatgtcgctg acgcgcagct cgtactcggc aaacggattg ggtcgcaggc gtttgatcgc
     6361 ctgtgagatc tttgtcggct cgtggaccag cctggcgaga attgcgtcct caacaaaacg
     6421 acattgccga gccgggaaac cgcgcagttg gcgttcggca tcttccgtaa tgacgatgtc
     6481 gtacgccatg agcagcggca atcaacacga atcagtgcga acaacgatcg gcaacgtcgc
     6541 tattctttcc cgttgttcgc tttcgccttt cccttcgccc cctccggcgg gaccttgtag
     6601 ccctcggcgt tgcgcttgtc ccattcggtg ccttcgtggg gcaggctggt cgcgcctttg
     6661 acgcggcgct cgagcgtctt catcgtctcg ttgaatgtgt acttgagata gtcgtcctgg
     6721 tcgtagagca gcgcctcgac cgccacgtcg atcgcggcct gcaagttgaa gaagctcaga
     6781 gcgcggacgg cctccagacg cacgcgggga tgctcgtcgg ccacctgcgc gcggagcaag
     6841 tcgagcggct tctccacgcg gtcgcgccag tagcccagca cccgcgtcgc cgcggcccgg
     6901 gcccggtaat cgggcgagcg gagcacctcc ttgaggagcg gctcgttgat ggcgtcgtga
     6961 gcctggtgca gccagagcgc ctccagcttg tggtgctcgt acagcggatc gtttttcggc
     7021 agggcagcca gccagcgtcc cgcttcgccg atgacctggt ccgtgggccg gttccacagt
     7081 tcgctccgca cccgctcgtg cgtccgcgct tccggctcgg tcttgagcag gtcgagcagg
     7141 accgcgatcg gctgcccagc gattttcggc ggcatgacca gcggcagctt cgtgtagtgc
     7201 acgcgccaga tgcggccgtg ggcgtggtcg cggttgggat cgcggatcga gtgctgcatg
     7261 tggccgatca gggggttgta ccagtcggcg acaaacagcg acccgtcggg ggcgaactcg
     7321 atatcgaccg gccggaagct tggatcgctc gactgcagca acggatcgac cgggtcggcg
     7381 gcgaagcccg agccctcgtc cttcatcttg tattgcagca cgccctgaaa cccgatgcag
     7441 ttattcagca ggtaattgcc ttgggcctcg ggcggaaagt tgcgactggt gaccaggtcg
     7501 cagccgcagg tcggccgcca ctgcttcgtg aggaacgttt tcagcgcgcc gtgcttgtgc
     7561 gggtaatcga cgtcgccgga gaaggccgcc ccaaaatagt tgaagcccgg cgaggcgtcg
     7621 gcgacgaagt tctgccccca gctgtcgaag atgtgccccc aggggttggc gaagccgtag
     7681 gagacgaaca cgtcgagctt gccggtccgc ggctcgtagc ggaagacgcc ggcgttggcg
     7741 cagcgctccg ggccataggg cgtctcgatc tgcgagtggt gaaatgtccc ttcttcgaaa
     7801 tagagcgccc cgccgggatc gagcgcgaac gcgctaatcg cgtggtgcga gtcggccgag
     7861 tcgaagccgt gcaggatcag ctcgcggacg tcggcctggt cgtcgccgtt ggtgtctttc
     7921 agaaacatga cgttcggctg ctgggcgatg atgatgccgc cgccccagaa ctcgataccg
     7981 gccggcaggt agagcttgtc ggcgaagact gtctgcttgt cggcccggcc gtcgccgttg
     8041 gtgtcttcca ggatcagcac cttgtcgttg ggcgggctgc ccggcaggta ctgcgggtag
     8101 ctgggcatcg tgcagaccca caatcggccc ttggcgtcga aggcgaaggc caccggcttt
     8161 tgcagatcgg ggaactcgcg ctccgaagcg aacaggttga ccgcgtagcc ctcgggcagc
     8221 gtaaacttct gctgggcctc ttcgggcggg ttgatggaga cgatgttctt gtagttcgtt
     8281 tcgatccgcg cgttttcgcc ggtcgagctg tcgtcgattg tctccggcac tttcttcccc
     8341 tgggcgatgt cccagattcg ccggtcgcgg acgtcgatca tcatccgcag cttggcgaac
     8401 tcgaccggga aattgaccac gccgaacggg ttcttgcggc cgccatagat atagaagccg
     8461 ttgaccgcgc ggtggtcgta gaagaactgc tggttctttt cgttgacggc agcccgcagc
     8521 ttcgacagat cgaccctcgc cgccggccgc gggccgaaca gggccttgtc gagaatctca
     8581 gcgatgaagg cgtcgccgtt ctcggccaga tggatgccgt tgatcgttag tgcctctttg
     8641 gagccgataa acagctcttg cgacggctga aacaaatcga cgaacggaac cttgtgtttc
     8701 gccgcgacct tcttcatcac cgcggtgtat aaggcgatgt tcttgttgtt cgccgtgcca
     8761 tcgggatagc cgcggcggtg gatgttctcg tgggcgatcg gactgatgag cgcgagctgc
     8821 ggcgggctcg tgccgttgta attcgtggcc aggatctcgg cgacgaactt gtccaagtcg
     8881 gcctcgaatt tcggcaaccc ctgctcgccg ccaaacgact cgttgaagcc gaagaacgcc
     8941 agaatcacgt cggccttgtg gtcaaacagc gtgtggccgt ggtcctgaaa cgcctgcgac
     9001 cggggccgca gggcgacctc gtcggccgac cagccgagat tccgaacgat cagctccaac
     9061 tcgggaaatc gcgcgtgcag gttcgtctca aagttgccga aatactgcat ccgctcggct
     9121 agcgtgttgc caatcagtgc gacgtgctgg cccttcgcca gctgcagccg cggctcatcc
     9181 actgcgcttg ccggcgcacc gatcaaaagg agagccaaca ccaccagata cgaaatcgtg
     9241 cgggacatag tccaccctcg cagaaattgc cagccggcga agttgccatt gtggcctgcc
     9301 gcgcgtgggc gttcaacggc cgctaagccg cgcgcggcga gaggatgagt cggcggccgc
     9361 ggtcgagatg cgcttcgagc agctgcgcgg cgctcgcgcc gtcgcccgcg atcgtcgctt
     9421 cggcaatcgc caggtgctcg ccgaagctcg tgtcgatccg cccgggattc agccggaaga
     9481 cgcgggtgat gacccgctgc accttgtcgc gcagctgccg catcacccgc aggatttcgc
     9541 gattgctgaa gaattcggcg aactgcatgt gaaactcggc gtccagctcg acgccgcgcg
     9601 caacgtcgcc cgtgccctga atctgccgct gcgcttcgag gttgccgcgg acccgtgcga
     9661 tctgctccgg cgtgagcttg cccgccaacg accgcagaat aaaggtctcc agtgcaacgc
     9721 gaatctcgta ctggtcggcg atctcctgaa tcgccaggtc gcggacgacg atgccttgct
     9781 gcggcgatat ggcgatcagc ccctcgaact gcagccgctc cagcgctgcc ttgaccggcg
     9841 tcttgctcat gccgagctgc tcggcgagct gccgctcggc gaggaacgac ccgggcggaa
     9901 actcgctgct gagaatccgc tctttgaggg cttcgtaggc ccggtccttc agcagctcgc
     9961 ggggtgatac agccgcgaca acgtcggcag gcattgaaaa tctcggcaag ggttcggaat
    10021 ccctgaactg gcccttgaat cttactggaa tctcagtaga atctcaaggc gttggggctc
    10081 gatttctctt agttttttcc cacccgactc atgtaccgtc ccatcgcctt gcggctgagc
    10141 ctggtgtcgc tgctgctact cctaattcat tgtgccgcgc gaagttgggc ggacgagccc
    10201 gcgccgtggg cgaaggcggc tgttcccgcc tttcccggag ccgaaggcgc tggcgcctgg
    10261 acgccgggcg ggcgcggcgg ccaggtgctc gtggtttcga gcctggaaga cagtggccca
    10321 ggcacgctcc gcgaggcgct ggccacgcgc gggccgcgga tcatcgtctt cggtgtggcc
    10381 ggaatcatca cgctcgcttc gccgctggag attagcgagc cctttgtcac gctcgctggc
    10441 cagaccgctc cgggcgacgg agtgtgcatt cgcggtgaat cgaccgcaat caacacgcac
    10501 gatgtggtta tccgctatct acggttccgc cgcggcaacc tgcagcgtcg cgacgacgcg
    10561 ctgggcggca acccagcggg caacgtgatc atcgaccacg tctcggccag ctggggcctc
    10621 gacgagaacc tttcgctgta tcgccacatg tcgccgctgc ccggcggcgg acagcggaaa
    10681 ggccccgtcg aaaacctcac gatccagtgg tcgatttcga gcgaggccct gaacctgaac
    10741 aaccacgcct tcggcggcac gtggggcgga aagaacagct cattccacca taatctgttc
    10801 gcctgcaaca cgggccgcaa tccgagcatc ggcatgggcg gcggattcga ctatcgcaac
    10861 aacgtgatct tcaactggat ccatcgcacg atggacggcg gcgacggcag ttcgcgggtc
    10921 aatgtgatcg ccaattacta caagccgggg ccggccaccg aaggcgagct gcgctaccgc
    10981 atctgcaagg cccaggcccg caactcccgt gatcagtttc ccggttttgg caagtggttc
    11041 gtcgagggca acgtcgtcga ggggaacgcg gccgtgacgg ccgacaattg ggccggcggc
    11101 gtgcagtacg acgcggccca ccgcgaccgc gacgagatta ttccggctgg aaccgaggcc
    11161 gcggtccgct cgcgcgaggc cttccccgcg gccagcgtca cgacgcactc cgcccaggaa
    11221 gcatacgagc tggtcctcgc ccacgccggc gcttcgctgc cgcggcgcga tccggtcgat
    11281 gagcgggtcc tggaatccgt ccgcaccgga aaacccgcct tcggcgtcgg catcctgcag
    11341 accccggccg acgtcggcgg ctggcccgag tacaaggcgg cggagactcc ccgcgacggc
    11401 gaccgcgatg gattgcccga tatctgggag aaatcgcatg gcctcaatcc gtccgacgca
    11461 gccgacagcg cgctcgacgg cgatcgcgac ggctatacga acatcgaaga attcctgaac
    11521 ggcaccgacc ctcgggaact ggtggattac acccggccgg aaaacaatcg cagcgccctc
    11581 caagctgctc cgaggtcgcg atgatccgtc ggctgctggc cagctcgatc ctcgccacca
    11641 ccgcggccat gcagctcgcg gcggccgatc cctcggcgcg attcgtctat cccgaccggg
    11701 ccggcaagct cgtctatgat gccgacgccc gcggcaatcg catcccggac ttctcgcact
    11761 gtggctatgg cgggggcggc gctgcaattc ccaatgttcc gacacgtgtc actgttccac
    11821 ccaaggttgg cgacgccacc agccgcattc aggccgcgat cgactatgtc tcgcgccttc
    11881 ccgccgatca gcagggcctg cgcggcgcag tgcagctcct ggccggcagg cactcgatcg
    11941 ccggccaact gcggattgca gccagcggcg tcgttctgcg cggacaaggt gcggaaggcg
    12001 ggggaacgac gctgctggcg acgggaaccg atcggcggac cctgatccgc gtcgccggta
    12061 agtccgatcg gcggatttcg ccagccgttc acgccgtggc cgatgagtac gttcccgtcg
    12121 gcgcctgcac gctgcgtttg aagagtaccg acggattgca agtcggcaac actgtgctcg
    12181 tcactcatcc cagcccgcgg gagtggatcg cggccgtggg aatgaaccgt tttccgtcgc
    12241 gcgacgtcgg ctcctggctc gactggcagc ccgggaaaat ggacgtccgc tgggaccgcg
    12301 taatcaccaa gatcgacggc gaagcaatca cgcttgacgc gccgttgacg accgcgctcg
    12361 atgcagcaac cggcgctgga accgtcgcca gttacgactg gccgggccgc attcgccagt
    12421 ccggcgtcga aaatcttcgc tgcgaatcgg ccttcgatcc ggcgaacccg ctggacgaag
    12481 aacacgcctg gggcgcgatc gggctggaga atgtgcagga cgcctgggtg cgaaacgtca
    12541 cggcagtcca tttcgccggc tcggcggtcg ccgtgtggga gagctgcaag caaatcacgg
    12601 tcgaggactg cacgtcgctc gcgccggtct cggagctggg cggctaccgc cggcacacgt
    12661 ttttcaccag cggccagcag acgctcttcc gccgctgccg cgctgagcag ggccggcacg
    12721 atttttccgt cggctatctg gcggccggcc ccaacgcctt tgtcgagtgc acggccgacc
    12781 aagcccacca atacagcggc ccgatcgaaa gctgggccag cggcgtgctt tacgacaacg
    12841 tcgtcatgga tggcggcggg ctggcgctga ccaatcgcga gacggccgcg caaggagcgg
    12901 gctgggcggc agcaaactgc gtcctctggc aatgcaccgc gccgctcgtc acctgccgga
    12961 tgccgcccac cgcgcaaaac tgggccatcg gctgctgggg cgagtttgtc ggcgacggac
    13021 attggcgctc gctcaacgag ttcgtcgaac ccaaaagcct gtacgctgcc cagttggccg
    13081 agcgcatggg cgctgcctcg gcccgcccgc cagttccacc cgcgaatac
//
LOCUS       DASRJB010000790         6837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1358024, whole genome shotgun sequence.
ACCESSION   DASRJB010000790 DASRJB010000000
VERSION     DASRJB010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358024"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFB80_20865"
     CDS             complement(<1..645)
                     /locus_tag="VFB80_20865"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HZN36299.1"
                     /translation="MNRDFTQLTWSPQLEEDVRRLTRLAVFEDLDRGQDWTTICLVTD
                     EARAAANVVARQAGVIAGLPAIGVMLDEMDIAADYQPKLSDGDSASAGTLVATLAGSA
                     RDLLTSERTILNLLGRLSGIATLSRQFVQAIAGTEARLYDTRKTIPGWRRLEKYAVRC
                     GGGHNHRTGLFDAILIKDNHLALGHNEHGAAAYTPAEAVRKARAFLAQQSAGEAP"
     gene            848..2074
                     /locus_tag="VFB80_20870"
     CDS             848..2074
                     /locus_tag="VFB80_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36300.1"
                     /translation="MSQPHFRFLHAGGFALDQPLFGFAEPPEPLGDLLIDAPYLAAQK
                     VFDAALEERVDFVVLTGDLVDLARPSPRAIALLLENFERLDAHGIATYWSGGRLDSPQ
                     DWPAAARLPGGVQVFPALAPEELSHFRGDRPAANIVGRSWHGTASVQVGDFKSDADGL
                     PTIVVAYGQAEAERLADQMVDYWALGGQPQRQTFGSARRVIHYAGSPQGRCPAENGPH
                     GCTLVHVAADRTIRSQFTPTDAVRWQTERIAVEADATLETVREQLADRVKQLRAEAES
                     RPQIVTWKIKGGEHLAGPAARRDLAAEWQAWLRREFSAGKPIVWTHAVEIDTPELPDA
                     WFDEESMLGDFLRNLRELAALEPAAVDLAQHIPQQHRTSALAALTSWSEEEHRTILYD
                     AALTGAELLGAGDREP"
     gene            2135..5809
                     /locus_tag="VFB80_20875"
     CDS             2135..5809
                     /locus_tag="VFB80_20875"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN36301.1"
                     /translation="MRIKNIHIDGFGVWNALAIDELSDQATVVYGPNEAGKTTLMQFV
                     RAVLYGFTPDRRRKYLPPVNGGKPGGELRIANEFGSFTVHRGSSPSDLPEDQGLVAII
                     DDRGEPRDAAQLDALLAGVDEPTYSNVFAVGLKEIQELGSLDDTAAADYLYRLTSGLD
                     RVSLVDVIRDVEAARERLLATADSEPSQIPQLLARKDKLRGQIEELQRQTRRWSDLAT
                     TRTNLIDEAATLDKQIVEIETASHVLRAALDVQGPWNLRSDIQRQEAILKDVRALPER
                     SVEKLDGINQRLNAGRKRLKKLGARRNQLKEDLASLEINKVLLSHAGRIESLHDQSQW
                     ILGLENQVARLKEEVAKLDGDIEAAVTGYRTSGHATLDELPRETVSTLKRPGQVVREE
                     NEKLDAAKQEADEAKRALDAAVQQYEQVSRTKHLPDLNQAIQEAGTKVSLLRKRIQVE
                     ERLDQLTRRRAELEVDSGELGSYEDAPLRVTALVGVFFALGVMLILIGTVGWGYGYVA
                     SGWPYWMLGCLITGGTVLGKLLLEKHSDENVSDSFRQLEQLRLQLGEAKRERDELDAE
                     VPATSGSLDSRLREAEIELRDLEKLIPVRTEREMAEQRHQAAQRRLAGAQEAVKEAKH
                     RWRNALRSVGLPEDYAPPKVKQVVKNNEQVLELRRRLGARKDELDQRTRELLVINNRL
                     QQILDDVRHPCASDQPIVRIRELIQAVIKEKETLALRDDVEKKLRRLTRHRDKIVAIL
                     RKASRQRHALLTLAGVESEKNFRQAAADWARVVDLRRQQAELTVRIQTTLAGQYDEDA
                     VAAVFKAEGRDIKGRWDQRQAQLAEIRTRLAQIHERRGACTQEMHSLAADKQLSRCVL
                     ELGTVEAQINDAIRRWKILTVIGRLLEAVRKRYETDRQPETLREASQYLGRLTGGHYT
                     RVWMPLDRRGLLVETDKGESLSLDVLSRGTREAVFIGLRLALTGSFARRGAMLPLVLD
                     DVLVNFDTERVRCAADVLCDFARQGHQVIMFTCHEHITDIFEDAAAEVRVLPSRDGSQ
                     RVRKRRKLALELPPPTPPLVPDEPQPVRILPTVDPNPLLAMAAEELLFDPVYPLPTKV
                     EPAAKPKREKRKKLKAHLPDYWPLAELPSEAPRPKPTIEFKLPDQWPLAELPQPPVSP
                     PTPPVAVQPETFEVLAYRGSADAWPAPAQPAGISTPQPAATIVFSPPEPTPPEPKFIA
                     RRERRRFTWESPEMYWEETD"
     gene            complement(5821..>6837)
                     /locus_tag="VFB80_20880"
     CDS             complement(5821..>6837)
                     /locus_tag="VFB80_20880"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZN36302.1"
                     /translation="AKAPLPPYYPETPVVRRDVANYYDNLTFTDKLIGQVLKEFDDDG
                     LAESTVVFFWGDHGWGMPRGKRWIYDSGTHTPLIVRWPGKLQPGSVNDELVAFLDFAP
                     TVLSLAGVQIPKHLQGRAFLGEQAGPKREYVYAARDRMDETLDIIRGVRDKRFHYIRN
                     YRPDLPYAQNIAYMNEMPTMQEWRRLAAAGELSGPQAIFFQPQKPVEELYDTQADPHE
                     IHNLAEDEKFAGQLARLRAAHEKWREETGDLGLIPEAELNEQRRPGGKWSMAANPEIT
                     INGGMATIRCATEGASIAYRHGESGRWLLYSAPVAIAKGQTVQAQACRLGFRDSQQVS
                     ATAE"
BASE COUNT         1204 a         2307 c         2269 g         1057 t
ORIGIN      
        1 cggcgcttcg ccggccgact gctgtgccag aaaggccctg gccttgcgga cggcctcggc
       61 gggcgtgtag gcagctgcac cgtgttcgtt gtgacccagc gcgaggtggt tgtccttaat
      121 gaggatcgcg tcgaagaggc ccgtgcggtg gttgtggccg ccgccgcagc ggacggcgta
      181 tttttcgagc ctgcgccagc cggggattgt ttttcgcgtg tcgtacaggc gggcttccgt
      241 gccggcgatc gcctggacga actgccgact gagcgtggcg atgcccgaca ggcggccgag
      301 caggttgaga atcgtccgtt cgctagtcaa caggtcgcgg gccgagccag ccagcgtcgc
      361 gacgagcgtg cctgcgctgg cgctgtcgcc gtcggaaagc ttgggctgat agtcggcggc
      421 gatgtccatt tcatccagca tgacgccgat cgcgggcagc cccgcgatca ctccggcttg
      481 ccgagccacg acgttggccg cggcgcgggc ctcgtcggtg accaggcaaa tcgtcgtcca
      541 atcctgaccg cggtccaagt cttcaaaaac cgccagccgc gtcaaacgac gcacatcctc
      601 ttcaagctgc ggcgaccagg tgagttgggt gaagtcgcga ttcatttcag atttcagatt
      661 caagatttga tgctagcagc tatttcagtt ttcagatctc accccaggtg cagcaaaatc
      721 tgaaatccta aatctgaaat ctgaaattcc cgctgccgcc tccttccgct acattacccg
      781 gtcacgggcg tctttcgcag cccgccaggc gctgccagag acgccgggcc acagggaggt
      841 ggcgctcatg tcgcaaccgc attttcgttt tctccatgcc ggtggctttg ccctggatca
      901 gccgctgttt ggctttgccg agcctccgga gccgctgggc gatctgctga tcgatgcgcc
      961 gtacctggcg gcgcagaagg tgttcgatgc ggccctggag gagcgcgtcg atttcgtcgt
     1021 tttgactggg gacctggtcg acctggcgcg gccgagcccg cgggcgattg cgctcctgct
     1081 cgaaaacttc gaacggctcg atgcgcacgg catcgcaacc tattggtccg gtgggcggct
     1141 tgactcgccg caggactggc ccgccgccgc ccggctgcct ggcggcgtgc aggtgtttcc
     1201 ggccctggcg cccgaggagc tctctcattt ccgcggcgac cggccggcgg ccaacatcgt
     1261 cggccgcagc tggcacggaa cggccagcgt ccaagtcggc gacttcaagt ccgacgccga
     1321 cggcctgccg accatcgtcg tggcctacgg ccaggccgag gccgaacggc tggctgacca
     1381 gatggtcgat tactgggccc tcggcgggca gccgcagcgg cagacctttg gctcggcgcg
     1441 ccgggtgatt cactacgccg gcagccccca gggccgctgc ccggcggaaa acggcccgca
     1501 cggctgcacg ctggtgcacg tcgctgccga ccgtacgatc cgctcgcaat tcacgccgac
     1561 cgacgccgtc cgctggcaga ccgagcggat cgccgtcgaa gccgacgcga cgctcgagac
     1621 cgtccgcgag cagttggccg atcgcgtaaa gcagctgcgg gccgaggcgg aatcgaggcc
     1681 gcagatcgtc acctggaaga tcaagggcgg cgagcacctg gccgggccag ccgcacggcg
     1741 cgacctggct gccgaatggc aggcctggct gcggcgcgag ttttcggccg gcaagccgat
     1801 cgtctggacg cacgcggtcg agatcgacac acccgagttg cccgacgcct ggttcgacga
     1861 agagtcgatg ctcggcgatt tcctccgcaa tttgcgcgag ctggccgcgc tcgagcctgc
     1921 cgccgtcgat ctggcgcagc acattccgca gcagcaccgc acatccgcgc tggcggcgct
     1981 gacgagttgg agcgaggaag aacaccgaac cattttgtac gacgctgcgc taacgggagc
     2041 ggagttgctc ggcgcgggag acagggaacc gtaggccgcg actccgtcgc gggccgcccg
     2101 ccacggagtg gcgggctacg ccaaggaggg ccacgtgcga atcaagaaca tccacatcga
     2161 cggcttcggc gtctggaacg ccctggcgat cgacgagctg tcggaccagg cgacggtcgt
     2221 ctatgggccc aacgaagcgg gcaaaacgac cctgatgcaa ttcgttcggg ccgtgctcta
     2281 cggctttacg cccgaccgcc gccgcaaata cttgccaccg gtcaacggcg gtaagcccgg
     2341 cggggagctg cggatcgcca acgaattcgg ctcgttcacg gtgcatcgcg gcagcagccc
     2401 gagcgacctg ccggaggacc aggggctggt agcaatcatc gacgaccgcg gggagccgcg
     2461 cgacgcggcg cagctcgatg cgctcctggc cggcgtcgat gagccgacct acagcaacgt
     2521 ctttgccgtc ggcctcaagg aaatccagga gctgggctcg ctcgacgaca ccgccgccgc
     2581 cgactatctc taccgcctca ccagcggcct ggaccgcgtc tcgctcgtcg atgtgattcg
     2641 cgacgtcgaa gcggcccgcg agcgattgct ggcgaccgcc gacagcgagc cgtcacaaat
     2701 accgcagctc ctggcccgca aggacaagct gcgcggccag atcgaagagc ttcagcggca
     2761 gacgcggcgg tggagcgacc tggcaacgac gcggacgaac ctgatcgacg aagccgccac
     2821 gctcgacaag cagatcgtgg aaatcgaaac cgcgtcgcac gtgctgcggg cggcgctcga
     2881 cgtgcaaggc ccgtggaacc tgcggagcga catccagcgg caggaggcca tcctcaagga
     2941 cgtccgcgcc ctgccagaac gctcggtcga aaagctcgac ggcatcaacc agcggctgaa
     3001 cgccggccgc aagcggctga agaagctcgg cgcacgccgc aaccagctca aggaggatct
     3061 ggcctcgctg gagatcaaca aggtcctcct tagccacgcg gggcggatcg aatcgctgca
     3121 cgaccagtcg cagtggattc tcggcctgga gaaccaggtc gccaggctca aggaagaagt
     3181 cgccaagctg gacggcgaca tcgaggccgc cgtcaccggc tatcgcacct ccggccacgc
     3241 gacgctcgac gagctgcccc gcgaaaccgt gagcacgctc aagcggccgg gccaggtcgt
     3301 ccgcgaggaa aatgaaaagc tcgatgccgc caaacaggaa gccgacgagg cgaagcgagc
     3361 cctcgacgcg gccgtgcagc agtacgagca ggtcagccgc acgaagcact tgcccgatct
     3421 gaaccaggcc atccaggagg cgggcaccaa ggtctcgctg ctccgcaagc gaattcaagt
     3481 cgaggagcgg ctggaccagc tcacccgccg ccgggccgag ctggaggtcg atagcggcga
     3541 actaggaagc tacgaagacg ccccgctgcg ggtcacagcc ctggtcggcg tcttcttcgc
     3601 cctcggcgtg atgctgatcc tcatcggcac ggttggctgg ggatacggct atgtcgccag
     3661 cggctggccc tactggatgc tcggctgcct gatcacgggc ggcaccgtgc tgggcaagct
     3721 gctgctcgaa aagcactcgg acgaaaacgt cagcgacagc ttccgccagc tggagcagtt
     3781 gcggctacag ttgggcgaag ccaagcgcga gcgggacgag ctcgacgccg aagtgccggc
     3841 caccagcggc tcgctcgact cgcggctgcg cgaagccgag atcgagctgc gggacctgga
     3901 aaagctgatt ccggtgcgca ccgaacggga aatggccgag cagcggcacc aggccgcgca
     3961 gcggcgactg gccggcgcgc aggaggccgt gaaagaagcc aaacaccgct ggcgaaacgc
     4021 cctgcggagc gtcggcctgc cggaagacta tgccccgccg aaggtcaagc aggtcgtgaa
     4081 gaacaacgag caggtgctgg agctgcgccg ccgcttgggc gcccgcaagg acgaactgga
     4141 ccagcgaacg cgcgagctac tcgtcatcaa caaccggctc cagcaaatcc tcgacgacgt
     4201 gcggcacccg tgcgcgagcg atcagccgat cgtgcgaatt cgcgagctga tccaggcggt
     4261 gatcaaggaa aaagagacgc tggcgctgcg ggacgacgtc gagaagaagc tccgccggct
     4321 cacgcggcac cgcgacaaga tcgtggcgat cctccgcaag gccagccgcc agcggcacgc
     4381 cctgctcacg ctcgccggag tcgagagcga gaagaatttt cgccaggccg cggccgactg
     4441 ggcccgcgtg gtcgatctgc ggcggcagca ggccgagctg acggtgcgga tccagacgac
     4501 gctcgccggt caatacgacg aagacgccgt cgccgcggtc ttcaaggccg agggccgcga
     4561 catcaagggc cgctgggacc agcggcaggc gcagctcgcc gaaatccgca cgcggctagc
     4621 ccagattcac gagcgccgcg gggcgtgtac tcaggagatg cactcgctgg ccgccgacaa
     4681 acagctttca cgctgcgttc tggagctggg caccgtcgag gcgcaaatca acgacgccat
     4741 ccgccgctgg aagattctca ccgtcatcgg ccggctgctg gaggccgtcc gcaagcgtta
     4801 cgagaccgat cgccagccgg agacgctccg cgaggcctcg cagtatctcg ggcggctgac
     4861 cggcgggcac tacacgcgag tctggatgcc gctcgaccgc cgcggcctgc tcgtagagac
     4921 cgacaagggc gaatcgctct cgctggacgt cctcagccgc ggcacgcgcg aagccgtgtt
     4981 catcggcttg cggttagcac tgaccggttc gttcgcccgc cgcggcgcga tgctgccctt
     5041 ggtgctcgac gacgtgctcg tgaatttcga caccgaacgg gtccgctgcg ccgcggacgt
     5101 gctctgcgac ttcgcccgcc aggggcacca ggtgatcatg ttcacctgcc acgagcacat
     5161 caccgatatc ttcgaggacg ccgccgccga agtccgggtg ctgcccagcc gcgacggcag
     5221 ccagcgcgtc cgcaagcggc gaaagctcgc cctagaactt cccccgccga ctccgccgct
     5281 ggttcccgac gagccgcagc cggtgcggat tctgccgacc gtcgatccga atcccctgct
     5341 ggcgatggcc gccgaagagc tgctcttcga tccggtctat ccgctgccaa ccaaggtcga
     5401 gccggctgct aagcccaagc gcgagaagcg caagaaactc aaggcgcacc tgccggacta
     5461 ttggcccctg gccgagctgc catcggaagc cccgcggccg aagccgacaa tcgagttcaa
     5521 gctgcccgat cagtggccgc tggccgagct gcctcagccg ccggtctcgc cgccgactcc
     5581 tccagtagct gtgcagccgg agacatttga agtgctggcg tatcgcggct cggcggacgc
     5641 ctggccggca ccggcgcagc ccgccggaat ctcaacgccg cagccggcgg cgacgatcgt
     5701 cttctcgccg cccgagccga cgccgcccga gcccaagttt atcgcccgcc gcgagcggcg
     5761 gcgctttacg tgggaatcgc cggagatgta ctgggaagag acggattaag gctgcgggct
     5821 tcattccgcc gtcgcgctga cttgctggct gtcgcggaat cccaaccggc aggcctgggc
     5881 ttgcaccgtt tgccctttcg cgatagcgac gggcgcggaa tagagcagcc accgccccga
     5941 ttcgccgtgg cgataggcga tcgacgcccc ttcggtcgcg cagcggatcg tcgccattcc
     6001 gccgttgatc gtgatctcgg gattggcggc catcgaccac ttcccgccgg gccgccgctg
     6061 ctcgttcagc tcggcctcgg ggatcaggcc caggtcgcca gtctcctccc gccacttctc
     6121 gtgtgcggcc cgcaggcgcg ctagctggcc ggcgaacttc tcgtcctcgg ccagattgtg
     6181 aatctcgtgc ggatccgcct gcgtgtcgta cagctcttct accggctttt ggggctgaaa
     6241 gaaaatcgcc tgcgggcccg agagctcacc ggccgctgcc agccgccgcc actcctgcat
     6301 ggtcggcatc tcgttcatgt aggcgatgtt ctgggcatag ggcaggtccg gccggtagtt
     6361 gcggatgtag tggaaccgct tgtcgcggac gccgcggata atgtcgagcg tttcgtccat
     6421 ccggtcgcgg gccgcataga cgtactcgcg ttttggtcca gcctgctcgc ctagaaacgc
     6481 acggccctgc aggtgcttgg gaatttgcac gccggccagc gagaggaccg tcggggcgaa
     6541 atcgagaaat gcgaccagct cgtcgttcac actccccggc tgcagcttgc ccggccagcg
     6601 aacgatcagc ggcgtgtgcg tgcccgagtc gtaaatccag cgcttgcccc gcggcatgcc
     6661 ccagccgtga tcgccccaga agaaaacgac cgtgctctcg gccaggccgt cgtcgtcgaa
     6721 ctccttcagc acctgcccga tcagcttgtc ggtaaaggtg aggttgtcgt agtagttggc
     6781 gacatcgcgg cgcacgaccg gcgtctcggg atagtagggc ggcagcggcg ctttggc
//
LOCUS       DASRJB010000791         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2759795, whole genome shotgun sequence.
ACCESSION   DASRJB010000791 DASRJB010000000
VERSION     DASRJB010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759795"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFB80_20885"
     CDS             complement(<1..995)
                     /locus_tag="VFB80_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36303.1"
                     /translation="MATAALRPSVSLVQLVLVGAVVPAAFVAADLWLVQRLNGGRESS
                     EAIAAFVVQIGVLGMLCARLRGPDWLRWVIYGWGFALIDAIELAYIGVDHSGNTAVLL
                     LISLLAAQLGLVIVWGALGTTPARVRWPVAVVLGVLVSTPVIGQEFHYNDDLGLLLLA
                     QAAALVVICGVLRLQGFRIALVARSVQEANQPAVTPKDLAQSQFGIRDVLIWTTSLAI
                     VLAVARILQLLSPARIAALFSREWIVLPAAGVLVAIALVVALWAALGAGPAWLRWLLL
                     LVVALAGGVALAVINWFVNGGPVWYDPNFPGPRREKWNWSMPFFRFLYDNHWEMI"
     gene            1083..1862
                     /locus_tag="VFB80_20890"
     CDS             1083..1862
                     /locus_tag="VFB80_20890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN36304.1"
                     /translation="MQPGRDLALVTGASSGIGAELARQLAARKIDLVLAARRRDRLQS
                     LADELSAQHGVQAAVIENDLNTAGGADQLIDELAARQLAPTVLINNAGFGYFSSFVEQ
                     PRDDIEALLQVNIRAVTVLSRRLGEAMAARKHGYILNVSSFAALAPIPRYAVYSGAKA
                     YVISWSQALRHELSRRGVMVSVLCPGFTRTEFHDVSRHEKSWIMRNTELTAPQVARAG
                     LNGLVRGKFLIVPGWWYKTMWLGASILPRWVTSAISARMVK"
BASE COUNT          386 a          692 c          663 g          292 t
ORIGIN      
        1 tcgatcattt cccaatggtt gtcatagagg aaccggaaaa atggcatcga ccagttccac
       61 ttttcgcggc gcggcccagg gaagttcgga tcgtaccaaa ccggaccgcc gttgacgaac
      121 caattgatca cagccaaggc cacaccgccg gctagcgcga cgaccagcag cagcagccac
      181 cgcagccagg ccgggccggc ccccagcgcc gcccacagcg cgacgaccag ggcgatcgcg
      241 accagcaccc ctgccgcagg cagcacgatc cactcccggc tgaacagtgc cgcgatgcgg
      301 gccggcgaga gcaactgcag gatgcgggcg acggccagca cgatcgccag cgacgtcgtc
      361 cagattagca cgtcgcggat gccgaactgc gactgggcca ggtccttcgg cgtcaccgcc
      421 ggctgatttg cttcctgaac ggatcgcgcc accagcgcga tccgaaaccc ctgcagccgc
      481 aagacgccgc agatcacgac cagagcggcg gcctgagcca gcaggagcaa tccgaggtcg
      541 tcgttgtagt gaaactcctg cccgatgacc ggagtgctga ccaggacgcc gagcacgacg
      601 gccaccggcc agcggacgcg cgcgggcgtc gtgcccagcg cgccccagac gatgaccagc
      661 ccgagctgag cggccagcag cgaaatcaag aggagcaccg ccgtgttgcc cgaatggtcc
      721 acgccgatat acgccaactc gatcgcgtcg atgagggcaa agccccagcc gtaaatgacc
      781 caccgcagcc agtccggacc ccgcagcctg gcgcacagca tgccgagcac gccgatttgc
      841 acgacgaagg ccgctatcgc ctcgctcgat tcgcggcctc cgttcaggcg ctgcaccagc
      901 cacagatccg ccgcgacgaa ggcggcgggc acgaccgccc caaccagcac cagttggacc
      961 agcgagaccg acggacgcag cgcggcggta gccataagcg gattgtagat tcgcccgcgg
     1021 caaaaatcgc cgcatctggt atcgtttggg cggccgctaa cccttggatt ttcaggcgcg
     1081 acatgcaacc gggacgcgac ttggctctgg tgacgggcgc ttcgagcggg atcggcgcgg
     1141 agctggcccg gcagctggcc gcgcggaaga tcgacctggt gctggcggcg cggcggcgcg
     1201 accggctgca gtcgctggcg gacgaattga gcgcgcagca cggcgtgcag gccgcggtga
     1261 tcgagaacga cctcaatacg gccggcggcg cggatcaact gatcgacgag ctggcggcca
     1321 ggcaactggc gccgacggtc ctgatcaaca acgcgggctt cggctacttc agcagcttcg
     1381 tggagcagcc gcgggacgac atcgaggcgc tgctgcaagt caacatccgg gccgtgaccg
     1441 tcctttcgcg ccggctgggc gaggcgatgg ccgccaggaa gcacggctac atcctcaacg
     1501 tgtcgtcgtt cgcggcgctg gccccgattc cgcggtacgc cgtctattcg ggcgcgaagg
     1561 cctacgtcat ctcgtggtcg caggcgctgc ggcacgaatt gtcgcggcgc ggcgtgatgg
     1621 tgagcgtgct ctgccccggc ttcacgcgga cggagtttca cgacgtctcg cggcacgaga
     1681 agagctggat catgcggaac acggagctca ccgcgccgca ggttgcccgg gccggtctga
     1741 atggcctggt gcgcggcaag ttcctgatcg tccccggctg gtggtacaaa acgatgtggc
     1801 tcggcgccag catcttgccc cgctgggtca cgtcggcgat ctctgcccgc atggtgaagt
     1861 aggcacaaag ccgcgaccct tggtcgcggt ctacggctcc accgatcaat gcgccaacga
     1921 caccaacgta ccgcgaccac tggtcgcggc ttggcaaggc gttttgccat tggtcgagcc
     1981 gttttccgcg attacacttc cgcccaacat gcgcgtcatt aaggacagaa tcg
//
LOCUS       DASRJB010000792         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3898900, whole genome shotgun sequence.
ACCESSION   DASRJB010000792 DASRJB010000000
VERSION     DASRJB010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3898900"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFB80_20895"
     CDS             complement(<1..737)
                     /locus_tag="VFB80_20895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN36305.1"
                     /translation="MSAPWYSGITRYQWLVLTIASLGWVFDVFEGQIFVASMNEALPS
                     LVPPGTSPETSKLYNNIALGAFLAGGALGGVVFGALGDRIGRARTMVITILVYSLFTC
                     LTAFAQTWWQVAVLRFLVALGVGGEWAVASAMVAEVFPPKARAWSGAIFHGSSVLGTF
                     LAVAAGAFIVNDPRLGWRWAFAVGALPALLTLWIRWQLKEPDAWVANRSAGAKPQAAG
                     RIRDLFAPGLWQRTLLGFLLAVVGLAT"
     gene            950..4930
                     /locus_tag="VFB80_20900"
     CDS             950..4930
                     /locus_tag="VFB80_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36306.1"
                     /translation="MPSREPQAAGATAVDQVLGYLNFSSGANDPQFLANLNRLFESAS
                     ADRGLPAWLAVSRQLTERLVMLRGSSPAFRDADQAAAVLELVFDRTLPAYREFHRDLL
                     FHRTDEALFRPFFVGRVCEAVLRQGPPWSEADRIVTAAIAQLNDYIGHRPVAVLESQK
                     LEANKHEFVRPIPLFIRGVGISCGPERAVIAAALQLLEETDPDLLRQACLDPKLIDEL
                     AVDPRAYDFDHPANKRPNYHFGQWDPHHIDNQGRYRRFVVQQVTLDALMRRLTDTPDL
                     TPDELLFEAAAVLAGTVLMASGISGEGPSSFDSTVTLSKLLPKIARYRDSFYEQLFHK
                     VSGPHGERLKSEAAERRQPFGGARQHLNAQLARQRASQLEHVHLARIFARMGHTAAAQ
                     REASIVPCAAARMQCQIDCLMTAGQVACDAGRLEEAAGLLPQIVDWLRRAIQCGAVID
                     PWNILGFDAHYSLFPALENSVRDHRADELVLQMDRLWALYSRVWSETAAVANTELSKK
                     VARQFKDTASWWHRFAVHEVSSVEAVDPLVVHRAAERVAQAMQVWHQGGAAAGDVAFW
                     APHAEMFDSPKAYALVIEALLDRGDYVATMALLVHWLGQADRIGLARADVSWHELAER
                     WFGEVTSLAGVDPADTAAARKWWDTARKFLDYLEANAESYWQPPQFRLGAKNGGKPAN
                     SLADDEEEDGDRFESAYDGVVYKDSTDDGIEGSIFETDESSADELAAESRRVADRLDF
                     LSTVACLWKHAAVSPAFLAVMQQDPVTLPAGCAADHAQRVTAIRRWIDQAEENRAALA
                     GLLDSVREYRIPTPSADHDAMVEYDRRRMTKESLLERVIATAVETAAAQRHLAAALAV
                     VADQPAGHPDDDQRHLVALLAALLQRDVAAAQEVCPQVRTLLAKQALLYVPLAKGGNP
                     HEIVTVRARQHALQDLLAWLPRLGLWIETCELLEVARQMEREHPVGPGAVTEYDELFK
                     IGYRSIVESLVVSAQAWPQHSASPEDAAESLVTCLEKLTESLLTGWLSHSRTLRLSVL
                     ERIHDRSAWKKVVAFVERYGDELFTQRFLNLSNIRAILHQGVAQWLTSLREDDSQDEG
                     PPRLLADLVAGDSTDEAAEQLTLVLEAIVENYGEYRDYNSTTTQSDRGELLYMLLDFL
                     RLRTRYDRVCWNLKPVVLAHEILVRRSEDQSARIWRKALSERINDEADQYLAKLAELQ
                     KKYAMRMPTVADRLAERFVRPLAIDRIRALVAGAIDEARRGAKDCPKFELLQIDTEYL
                     TREPTGVGLDVPAWLVALEEEVEEALRPAYQQREDQDLAAVIPPVLLTYDQARSQLEQ
                     WASRQA"
     gene            complement(4967..5332)
                     /locus_tag="VFB80_20905"
     CDS             complement(4967..5332)
                     /locus_tag="VFB80_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36307.1"
                     /translation="MSLSLAPRIRPAAMFDRPAQRLAAAFFALWVFIVFVSVFDGYLV
                     FRFRNLIHSTELNPLGLWLITANGGQVWALLGIKFAGTILACSLLLLVYWKNARLGTL
                     IASALAALQLSLLLFLLLG"
     gene            complement(5342..>5567)
                     /locus_tag="VFB80_20910"
     CDS             complement(5342..>5567)
                     /locus_tag="VFB80_20910"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36308.1"
                     /translation="ITGGESVDSLAVWNLRTMRMERELHGHEKAINGVSFLDERSLCS
                     FGADGTIRIWNLDSDAPPRVLTIDVTAQAS"
BASE COUNT          959 a         1912 c         1869 g          827 t
ORIGIN      
        1 aacgtcgcca gccccaccac cgccagcaag aaccccagca gcgttcgctg ccatagtccc
       61 ggagcaaaca ggtcgcgaat tcgccccgcg gcttgcggct tggcaccggc cgaccgattc
      121 gccacccaag cgtccggctc cttcaattgc cagcgaatcc agagcgtgag cagcgccggc
      181 agcgcgccga cggcgaatgc ccaccgccag cccagccgcg gatcgttgac gatgaacgcc
      241 ccggcggcca ctgcgagaaa cgtgcctagc acgctcgacc cgtgaaagat cgcgccggac
      301 caggcccggg ccttcggcgg aaagacctcg gccaccatcg cgctggccac cgcccattct
      361 ccgcccacgc ccagtgcaac cagaaaccgc agcactgcca cctgccacca ggtctgggca
      421 aaggccgtga ggcaggtgaa tagcgagtac accaggatcg tgatcaccat cgtccgcgcc
      481 cggccgatcc ggtcgccaag cgctccgaag acgacgcccc ccaacgcccc gccggccaga
      541 aacgcgccca gggcgatgtt gttgtagagc ttgcttgttt ccgggctcgt tcccggcggc
      601 accagcgacg gcagcgcctc gttcatgctg gcgacaaaga tctgcccctc aaacacgtcg
      661 aacacccagc ccagcgaggc gatcgtcagc acgagccatt gatagcgcgt gatgccggaa
      721 taccaagggg cggacatagg gttcagggtt ccgggctcgg ggcacacgac ggagagacgg
      781 ggagaaggag cgacggagcg accatccctc cttcgctctg tctctctttc gctccgtact
      841 tttgatccgc tggaccgttt ggcgacgtgc acttacgatt gaggtttgag cgggcggcgg
      901 ccggatccag cgggtccgcg caagtcgagc cgccgctgga gtttatggca tgccctctcg
      961 agaaccgcaa gccgccggcg ccaccgccgt cgatcaggtt ctcgggtatc tcaacttctc
     1021 gtcgggggca aacgaccctc agttcctggc aaacctcaac cgcctgttcg aatcggcttc
     1081 ggcggacagg ggtctgcccg cctggctggc cgtcagccgg cagctgacgg agcggctggt
     1141 gatgctccgg ggcagctcgc ccgccttccg cgacgccgac caggcggcag cggtgctgga
     1201 gctggtcttc gaccgcacgc tgccggccta tcgcgagttt caccgcgatc tcttgtttca
     1261 tcgcaccgac gaggcgctgt tccggccgtt cttcgtcggc cgagtctgcg aagcggtgct
     1321 gcggcagggc ccgccctgga gcgaggccga ccggatcgtg acggcggcga tcgcccagct
     1381 gaacgactac atcggtcacc gtccggtggc cgtgctggaa tcgcaaaagc tggaggcgaa
     1441 caaacacgag ttcgtgcggc cgatcccgct gttcatccgc ggggtgggca tctcgtgcgg
     1501 accggagcgg gcggtgatcg ccgcggccct ccagctgctc gaagagaccg atcccgatct
     1561 cctgcggcag gcttgcctcg atcccaagct gatcgacgaa ttggccgtcg atccgcgggc
     1621 ctacgacttc gaccatccgg ccaacaagcg gcccaactat cacttcggcc agtgggatcc
     1681 gcaccacatc gacaaccagg gccgctaccg ccgcttcgtc gtgcagcagg tcacgctcga
     1741 cgccttgatg cggcggctga cggacacgcc cgacctgacg ccggacgagc tgctgttcga
     1801 agcggccgcg gtgctcgccg gcacggtgct gatggccagc ggcatcagcg gggaagggcc
     1861 cagcagcttc gactcgaccg tcacgctcag caagctgctc cccaagatcg cccgctaccg
     1921 cgactcgttt tacgagcagc tgtttcacaa ggtcagcggc ccgcacgggg agcggctgaa
     1981 gtcggaagcg gccgagcggc ggcagccgtt cggcggcgcg cggcagcacc tgaacgccca
     2041 gctcgcccgg cagcgggcgt cgcagctgga gcacgtccac ctggcccgca tcttcgcccg
     2101 catgggccac acggcagcgg cccagcgcga ggccagcatc gtcccctgcg ccgcggcgcg
     2161 gatgcagtgc cagatcgact gcctgatgac cgcggggcaa gtcgcttgcg acgccggccg
     2221 gctggaagag gcggcggggc tcttgccgca gatcgtcgat tggctgcggc gggcgatcca
     2281 atgcggcgca gtgatcgacc cctggaacat cctgggcttc gacgcgcact acagcctgtt
     2341 cccggcgctg gagaacagcg tccgcgatca ccgggccgac gagttggtgc tgcaaatgga
     2401 tcggctgtgg gccttgtatt cgcgggtctg gagcgagacg gccgcggtcg ccaacacgga
     2461 gctctcgaag aaggtcgcgc ggcaattcaa ggacaccgcg agctggtggc accgcttcgc
     2521 cgtgcacgaa gtgtcgagcg tcgaagcagt cgatccgctc gtggtccatc gcgcggccga
     2581 gcgggtggcg caggcgatgc aggtctggca tcagggaggc gcggcggcag gcgacgtggc
     2641 gttctgggcg ccgcacgccg agatgttcga ctcgcccaag gcctacgccc tggtgatcga
     2701 ggccctgctc gaccgcggcg actacgtggc aacgatggcc ctcttggtgc actggctggg
     2761 acaggccgac cgcatcggcc tcgcgcgggc cgacgtctcg tggcacgagc tggccgagcg
     2821 gtggttcggc gaggtcacgt cgctggccgg cgtcgatccg gccgataccg cggcggcccg
     2881 caagtggtgg gacacggccc gcaagttcct cgattacctg gaggccaacg ccgagagcta
     2941 ctggcagccg ccgcagttcc gcctgggcgc gaagaacggc ggcaaaccag ccaactcgct
     3001 ggcggacgac gaggaggagg acggcgaccg gttcgagtcg gcttacgacg gcgtggtcta
     3061 caaggactcg accgacgacg gcatcgaggg ttcgatcttc gagaccgacg aatcgtcggc
     3121 cgacgagctg gcggccgagt cgcggcgcgt cgccgaccgg ctcgactttc tcagcacggt
     3181 cgcctgcctg tggaagcacg ccgccgtcag cccggcgttt ctggccgtca tgcagcagga
     3241 cccggtgaca ctgccggcgg gctgcgcagc cgaccacgcg cagcgggtga ccgcgatccg
     3301 ccggtggatc gatcaggccg aggaaaaccg cgccgcgctc gctggtctgc tcgattccgt
     3361 ccgtgaatac cgcattccca cgccgtcggc cgatcacgac gcgatggtcg aatacgaccg
     3421 ccgccggatg accaaggaat cgctgctgga gcgggtcatc gccaccgccg tagaaacggc
     3481 cgccgcccag cggcacctgg ctgccgcgct ggcggtcgtc gcggaccagc cggccggcca
     3541 tcccgacgac gaccagcggc atctcgtggc gctgctggcg gccttgctcc agcgcgacgt
     3601 ggccgccgcc caggaggtgt gtccgcaggt ccgcacgctg ctggccaaac aggccctgct
     3661 ctacgtcccg ctggccaaag ggggcaatcc gcacgaaatt gtcaccgtcc gcgcccgcca
     3721 gcacgcgctc caggatctgc tggcctggct gccgcgactg ggcttgtgga tcgaaacctg
     3781 cgagctgctc gaggtcgccc gccagatgga gcgcgagcat cctgtcggtc ccggggcagt
     3841 taccgaatac gacgagctgt tcaagatcgg ctaccgctcg atcgtcgaaa gcctcgtcgt
     3901 ctctgcccag gcctggccgc agcattccgc cagccccgaa gacgccgccg agtcgctcgt
     3961 cacctgcctg gagaagctta ccgagtcgct gctcaccggc tggctctcgc acagccgcac
     4021 gctgcggctc tcggtcctgg agcggatcca cgaccgctcg gcctggaaaa aggtcgtggc
     4081 ctttgtcgag cgctacggcg acgagttgtt cacgcagcgg ttcctcaacc tgagcaacat
     4141 ccgggcgatt ctgcaccagg gggtggccca gtggctgacg agcctgcgcg aggacgattc
     4201 gcaagacgaa ggcccgccgc ggctgctggc cgacctggtc gcgggcgact cgaccgatga
     4261 ggccgccgag cagctcaccc tggtgctgga ggcgatcgtc gaaaactacg gcgagtaccg
     4321 cgactacaac agcaccacga cccagtcgga ccgcggcgaa ttgctgtaca tgctgctcga
     4381 cttcctgcgg ctgcggacgc gctacgaccg cgtctgctgg aatttgaagc cggtggtgct
     4441 ggcccatgag attctcgttc gccgcagcga ggaccagtcg gcccgcatct ggcgaaaggc
     4501 cctcagcgag cggatcaacg acgaagccga tcagtacctg gcgaagctgg ccgagctgca
     4561 aaagaaatac gcgatgcgga tgccgacggt cgcggaccgg ctggccgagc ggttcgtgcg
     4621 gccgctggcg atcgaccgca tccgggcgct ggtcgccggg gcgatcgacg aggcccgccg
     4681 cggggcgaag gattgcccga agttcgagct gctgcaaatc gacaccgaat atctcacccg
     4741 cgagccgacc ggcgtcggcc tggacgtgcc ggcctggctg gtcgccctgg aagaggaagt
     4801 cgaagaagcc ctgcgcccgg cgtatcagca gcgcgaggac caggacctcg ccgccgtcat
     4861 cccgcccgtg ctgctcacct acgaccaggc ccgctcccag ctcgagcagt gggccagccg
     4921 gcaggcgtag ctcggtgggc cggttgatcg gtggtccgtc agccggtcat cccagcagca
     4981 aaaacagcag caggctcagt tgcagcgcgg ccagtgcgga ggcgatgagc gtgcccaggc
     5041 gggcgttctt ccaatagacg agcagcagca ggctgcaggc caggatcgtg ccggcgaact
     5101 tgatgccgag cagcgcccag acttgcccgc cgttggccgt gatcagccac aggccgagcg
     5161 gattgagctc cgtcgagtgg atcaggttgc ggaagcggaa gaccaggtag ccgtcgaaca
     5221 ccgacacgaa aacgatgaac acccacaggg cgaaaaaggc cgcggccagg cgctgggcgg
     5281 gacggtcgaa catggcggcg gggcgaatgc gaggcgcgag gctcagcgac atgaatcgct
     5341 cctaactggc ctgggcggtg acgtcgatcg tcagcacgcg cggcggtgcg tcgctatcca
     5401 aattccaaat gcggatcgtg ccgtccgcgc cgaacgagca gaggctccgc tcgtccagga
     5461 agctgacgcc gttgatcgct ttttcgtggc cgtgcagctc ccgctccatg cgcatcgtcc
     5521 gcaggttcca gaccgccaga gaatcgacac tctcgccgcc cgtgatc
//
LOCUS       DASRJB010000793         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2491773, whole genome shotgun sequence.
ACCESSION   DASRJB010000793 DASRJB010000000
VERSION     DASRJB010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491773"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFB80_20915"
     CDS             <1..609
                     /locus_tag="VFB80_20915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36309.1"
                     /translation="GKTLVATLHAPELRAAVTVDGIRQRLAKYPPAVQTEAEKLYAEI
                     DAELATQRSRLEATLAELPTGDIRRGQAVFNSTKLSCRACHTIGYVGGKSGPDLTRIG
                     QIRQPRDLVESILFPSASFVRSYEPVAVRTLDGQVFSGVVKVDSPVEVILTLAADKEV
                     RIAREEIEIAQPGRVSIMPAGLDKQLSLQELADLVEFLRNCR"
     gene            655..1635
                     /locus_tag="VFB80_20920"
     CDS             655..1635
                     /locus_tag="VFB80_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN36310.1"
                     /translation="MIPGLRLASGDALPVVGLGTWKVAKERAAELVVEAIRAGYRHFD
                     CACDYGNEPQIGEGLRTAIQNGPCRRDELWVTSKLWNTYHAAEHVRPACERTLRDLGL
                     DYLDLYHVHFPIAQRFVPFESRYPPEWFFDPQASQPRMEPANVPIRETWGAMEELVRA
                     GLVRNIGVCNFGVSLLRDLLSYCKIRPAVLQVELHPLLTQDKLLRFCRESGLAVTGFS
                     PLAAESYFSIGMAQPQESVLRKEVVIDAAQRHGKSRAQVVLRWAVQRGTAIVPKTART
                     ERLRENLALFDFELSPDEMQAISGLNRGRRFNDPGEFCERAFGTFFPIYE"
     gene            1663..>2053
                     /locus_tag="VFB80_20925"
     CDS             1663..>2053
                     /locus_tag="VFB80_20925"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN36311.1"
                     /translation="MTRITRIEIAGQQTHGGEPFPLAFACQDAAIDLPATTDFVRGWR
                     DDLLKAAARHGAVLFRGFPLRTAADFDAFVAAFDLPNFPYDESLSNAVRVNKTPRVFT
                     ANEAPPSVTIFLHHEMAQTPIYPSRLFF"
BASE COUNT          356 a          655 c          692 g          350 t
ORIGIN      
        1 ggcaagacgc tggtagcgac gctgcatgcg ccggagctgc gggcggcggt gaccgtcgat
       61 ggcatccgcc agcggctggc caagtatccg ccggcggtcc agacggaggc ggagaagctt
      121 tatgccgaaa tcgacgccga gctggcgacg cagcgctcgc ggctggaggc gacgttggcc
      181 gagctgccca cgggagacat ccgccgcggg caggccgtgt tcaacagcac gaagctttcc
      241 tgccgggctt gccatacgat cggctacgtg ggcggcaaga gcggccccga cctcactcgc
      301 atcggccaga ttcggcagcc gcgggacctg gtcgaatcga tcctgttccc cagcgcgagc
      361 ttcgtccgca gctacgagcc ggtcgccgtt cgcacactcg acgggcaagt tttcagcggc
      421 gtggtgaagg tcgattcgcc ggtcgaggtc atcctcacgc tcgccgccga caaggaagtc
      481 cgcatcgctc gcgaggagat cgaaatcgcc cagcccggca gggtctcgat catgccggcc
      541 ggcctcgaca agcagctctc gctgcaggag ctggccgacc tggtcgagtt cctccgcaac
      601 tgccggtgag ggccacggcc acgaaaaaca cgacgagcac aaagagcacc aaggatgatt
      661 ccgggattgc gattggcgag cggcgatgcc ctgccggtgg tcggactcgg aacgtggaaa
      721 gtggcgaagg aacgggcggc ggagttggtg gtggaggcaa tccgggccgg ctatcggcat
      781 ttcgactgcg cctgcgacta tggcaacgag ccgcagatcg gcgaggggtt gcggacggcg
      841 atacaaaatg ggccgtgccg ccgcgacgag ctgtgggtca cgtcgaagct ctggaacacg
      901 taccacgcgg ccgagcatgt gcggccggcg tgcgagcgga cgctccgcga tctggggctc
      961 gactatctcg acctgtatca cgtccatttt ccgatcgccc agcggttcgt gccgtttgag
     1021 tcgcgctatc cgccagagtg gttcttcgat ccgcaagctt cacagccgcg aatggagccg
     1081 gccaatgtgc cgatccgcga aacgtggggc gcgatggaag agctggtccg cgccgggctg
     1141 gtgcggaaca ttggcgtctg caacttcggc gtgtcgctgc tccgcgactt gctctcgtat
     1201 tgcaagatcc gccccgccgt cttgcaagtg gagctgcatc cgctgctcac gcaggacaaa
     1261 ctgctgcgat tctgccgcga gagcggcctc gccgtcacgg gcttctcgcc gctcgccgcc
     1321 gaatcgtact tctcgatcgg catggcgcag ccgcaggagt cggtgctgcg aaaagaggtg
     1381 gtcatcgatg cggcgcagcg acacggcaag tcgcgggcac aggtcgtgct ccgctgggcc
     1441 gtgcagcgcg ggacggcaat cgtgcccaag acggcgcgga ccgaacggct gcgagagaac
     1501 ctcgcgctgt tcgacttcga gctgagcccg gacgagatgc aggccatcag cggcctcaat
     1561 cgcggccggc ggttcaacga tccgggcgag ttctgcgaac gggcgttcgg cacgttcttt
     1621 ccaatctacg agtagttgca ggcaataatg ggaggaccgc ggatgacgcg gatcacgcgg
     1681 attgagattg caggtcagca gacgcacggc ggcgagccgt ttccgcttgc attcgcctgc
     1741 caggatgcgg caatcgatct cccggcgaca accgatttcg ttcgcggttg gcgcgacgac
     1801 ctgctgaagg ccgcggcccg gcacggcgcg gtcttgttcc gcggttttcc gctccgtacc
     1861 gccgcggact tcgacgcctt cgtggcggcg ttcgatctgc cgaactttcc ctacgatgag
     1921 tcgctctcca acgccgtccg cgtcaataag acgccacggg tcttcacggc caacgaagcc
     1981 ccgccgagcg tgacgatctt tctgcaccac gaaatggccc agacgccgat ctatccgtcg
     2041 cggctgttct tct
//
LOCUS       DASRJB010000794         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3337459, whole genome shotgun sequence.
ACCESSION   DASRJB010000794 DASRJB010000000
VERSION     DASRJB010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337459"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="VFB80_20930"
     CDS             complement(<1..1991)
                     /locus_tag="VFB80_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36312.1"
                     /translation="MRVQIIALAVGLGWTLAAAALAAADTAPDFNRQVRPILSAHCFK
                     CHGPDESVREAGLRLDRRDVATKELDSGVTAIVPGKPESSEVVRRVFSADKDEQMPPP
                     AANKPLSAAQKEILRRWIAAGAEYQQHWSFVAPKAGPLPPTSHAAANAIDAFIQAKLA
                     AAGLQPSPPADRYTLVRRASLDLIGLPPSIEEADAFVNDPAPDAYERLIDRLLASPAY
                     GERWARRWLDLARYADTNGYEKDRPRSMWPYRDWVIRALNTDLAFDQFTIEQLAGDML
                     PSAATEQATQDQRIATGFHRNTMINEEGGIDPLEFRFYAMVDRVNTTSTVWMGLTLGC
                     AQCHTHKFDPIPHADYYRQLALLNNADEPLLDVLAPEIAAQRAGIEQQIAEFTADLPK
                     TFPPEDQWQWQTPRPAEFTSAGDASGTIREDGSILVGGKNPDEDTYTIAFDAPAGEYA
                     ALRLEVLVDPALPGTGPGRTPHGNFVLSEIKLQAGDTPVKLASAEADAAQEGFPAARA
                     IDGDAKTGWAIHVAGAWNVNRSWTGRFEQPLKLAQPVRCTVRLEQQHGQQHTLGCFRL
                     SLGRPASADGRPLAERSREHLEKKFAAWLEAERLRTVAWQALKPLSAQSQRPTLTIQD
                     DRSIFSSGDITKRDVYELTFDTAELKGITALKLEVLPDER"
     gene            2160..4520
                     /locus_tag="VFB80_20935"
     CDS             2160..4520
                     /locus_tag="VFB80_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36313.1"
                     /translation="MRFNVAIVFVTTLLASASAAQEAESVSQIAEALKKISPHVAAEA
                     ERDQLRGMLGRSLREQIAAGNRASSEAWGKIQSREDWERFRAEKLALLRKSLGTLPPR
                     PARPKTLVTGRIDGDGFQIQNLVYESRPGLVVTANLYVPVPARDKMPGIAIAHSHHNP
                     KWEGELQDMGMTWARAGCYVLVPDALGHGERRQHPFAAAADYSQEFPVSRQDYNFRYD
                     TSLQLYLAGETLMGWMAHDLMTGFDVLVNQPGIDDTRLILLGSVAGGGDPCGVTGALD
                     ERIDCIVPFNFGGPQPENRYPLPADAETSFNYAGGGSWESTRNLYRSAVDGFLPWVIV
                     GSVAPRKLIHAHEFSWDRERDPVWKRYEKIYGLYGAKDNLAFTTGFGTIQNTQAPASH
                     CNNIGPPHRKLIHEALGKWFGIKVTPESEYKNRRTREELTCLTDEARKQFQPKLLHEL
                     LAKMADQQLAAAQKSRAAMKPDERRDQMRDVWTRLLSDTEPPKEIKVRDGSPATEQVG
                     GVTVRRELLETAPGISVPLMTLSLSRLDDRKDRSRFAILGLAGDGLQAALPRRNKDIA
                     EGLLAGCVIALVDVRGLGAAGSEQDRGQQSSSTGHSATQLMLGQTMLGGQLRDLRAAW
                     QHLQRREDVSRNVVIAGGSGREPLAADAEFKYPRRVDRPAESQPEAALLSLLFGLYEP
                     ASTVIVSRHGLVSYRSILDSPFVQAPHAAIVPGALQLVDLPDLAVVLAPREVVLDVLV
                     DGRGRLIPKAVAEATYEAAARANTGSERPASLQISETDSPLVGRQP"
     gene            4517..5014
                     /locus_tag="VFB80_20940"
     CDS             4517..5014
                     /locus_tag="VFB80_20940"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN36314.1"
                     /translation="MKRSSPANPQIAALLQLLDEAYEKQAWHGPNLRGSLRGIEPAQA
                     AWRPAAGRKNIWEIALHAAYWKYACRRRLAGEKRGSFPLKGSSWFERGDASDERQWVG
                     DLALLERMHRELRAVVAAYPPTKLLVRIAPTKHTPLRLIQGVALHDVYHTGQIQTLKR
                     LYSQS"
     gene            5203..>5690
                     /locus_tag="VFB80_20945"
     CDS             5203..>5690
                     /locus_tag="VFB80_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36315.1"
                     /translation="MSLVRRPLLAAALFALLFPAIARAQSSEINVTGKKAAYSLSDET
                     GLVSQQPDLVPEPPPLPIAPQRSPSDQPSVLAPSYNVSGGDKYTCCDSCDSCCGCGFL
                     SSWPCGCPLECLGEACKLWEPCCEDSPWSGAGWLAAGFVWNPYSPVDRFNGPMTWNDR
                     AN"
BASE COUNT          952 a         1896 c         1922 g          920 t
ORIGIN      
        1 cgctcgtcgg gcagcacttc cagcttgagg gcagtaatgc ccttcagctc ggccgtgtcg
       61 aatgtcagct catagacgtc gcgcttggta atgtcgccgc tggaaaagat ggagcggtcg
      121 tcctgaatcg tcagcgtcgg ccgctggctc tgggccgaca gtggcttgag cgcctgccac
      181 gcgacggttc gcaagcgttc agcctccagc caggccgcga acttcttttc caggtgctcg
      241 cggctgcgct cggccagcgg ccggccatcg gcgctggcag gccggcccaa actcagccga
      301 aagcagccga gcgtatgctg ctgtccgtgc tgttgctcga gccggacggt gcagcgcacc
      361 ggctgcgcca gcttcagcgg ctgctcgaag cggccggtcc acgaacgatt gacgttccac
      421 gcacccgcga cgtgaatcgc ccagccggtc ttcgcatctc cgtcgatcgc gcgggcggca
      481 ggaaagccct cctgagccgc gtcggcctcg gccgaggcga gcttgactgg cgtatcgccc
      541 gcttgcagct taatctcgct gaggacgaaa ttgccgtgag gtgtccgacc gggcccggta
      601 ccgggcagcg ctggatcgac gagcacctcc agccgcaggg ccgcgtactc gccggcaggc
      661 gcgtcgaagg caatcgtgta ggtgtcctcg tccggattct tgccgccgac gaggatggag
      721 ccatcctcgc gtatcgtgcc gctcgcgtcc cctgcggacg taaactcggc cggccgcggc
      781 gtctgccact gccactggtc ctcgggcggg aaggtcttag gcaagtcggc ggtgaactcc
      841 gcgatctgct gctcgatgcc ggcccgctga gcagcgatct ccggcgcgag cacgtcgagc
      901 agcggctcgt cggcgttgtt gagcagggcg agctgccggt aatagtcggc gtgcggaatc
      961 gggtcgaact tgtgcgtgtg gcactgggcg cagccgagcg tcagccccat ccagacggtg
     1021 ctggtcgtgt tgacccgatc gaccatcgcg tagaagcgaa actccagagg atcgatgccg
     1081 ccctcttcgt tgatcattgt gttgcggtga aagccggtag cgatccgctg gtcctgcgtg
     1141 gcctgttcgg tagcagcact gggcagcatg tcgccggcga gctgctcgat cgtgaactgg
     1201 tcgaacgcca ggtcggtgtt gagcgcacgg atgacccagt cgcggtaggg ccacatcgat
     1261 cgcgggcggt ccttttcgta gccgttggtg tcggcgtagc gggccaggtc gagccagcgg
     1321 cgggcccacc gctcgccata cgcgggcgac gccagcagcc gatcgatcag ccgctcgtag
     1381 gcgtccggcg cgggatcgtt cacaaaagcg tcggcctctt caatcgatgg cggcaggccg
     1441 atgaggtcga gcgaggcgcg gcgaacgagc gtgtagcgat cggccggcgg tgagggctgc
     1501 aggcccgccg cggcgagctt cgcttgaatg aaggcgtcga tggcattggc ggctgcgtga
     1561 gacgttggag ggagcggacc cgccttgggt gcgacaaacg accagtgctg ctgatactcg
     1621 gccccggccg cgatccagcg tcggagaatc tccttctgtg cggccgagag cggcttgttg
     1681 gcagccggcg gcggcatctg ctcgtccttg tcggccgaga aaacccgtcg caccacctcg
     1741 ctgctctccg gctttccggg gacgatggca gtcacgccgg aatccagctc tttcgtggcg
     1801 acgtcccgcc gatccagccg caagccagcc tcgcgcacgc tctcgtccgg gccgtggcac
     1861 ttgaagcagt gagccgaaag aatcgggcgg acctgacgat tgaagtcagg cgcagtgtca
     1921 gcggcggcta gcgccgctgc cgccaatgtc cagccgagcc cgaccgccaa cgcaattatc
     1981 tggactcgca tcgttccgcc tcgccaggac atggccacag gactcttcat catattctgg
     2041 cagggcgaaa cccgacaccg caccggcgac tctccgcaaa tggattcagc cttcgcagtg
     2101 cggtatgctg tggcgcgatc ggggtccgcg tttcggtgct tcgtgctgga gcaattgcca
     2161 tgagattcaa tgtcgccatt gtctttgtca ccacgctgct ggcgtcggca tcggccgcgc
     2221 aggaggcgga gagcgttagc cagattgccg aggctctcaa aaagatatca ccgcacgtcg
     2281 ccgccgaagc cgagcgcgat cagctgcgcg gcatgttggg ccgctcgctg cgcgagcaga
     2341 tcgccgccgg caatcgggcc agctcggaag cctggggcaa gattcagtcg cgtgaggact
     2401 gggagcgctt ccgggcggag aagctcgcgc tgctgcggaa gtcgctgggc acgctgccgc
     2461 cgcgaccggc caggcccaag acgctcgtga ccgggcgaat cgacggcgac ggcttccaga
     2521 ttcagaacct ggtctatgaa tcacggccgg gccttgtcgt cacggccaac ctctacgtcc
     2581 cggttccggc gcgggacaag atgcccggca tcgccatcgc ccacagccac cacaacccca
     2641 agtgggaagg cgagctgcag gacatgggta tgacttgggc ccgtgccggc tgctacgtcc
     2701 tggtgcccga cgccctcggc cacggcgagc ggcggcagca tcccttcgcc gccgcggccg
     2761 actattccca agagtttccc gtcagccggc aggattacaa cttccgctac gacaccagcc
     2821 tccagctcta tctcgccggc gaaacgctga tgggctggat ggcccacgac ctgatgacgg
     2881 gcttcgacgt gctggtgaac cagccgggca tcgacgacac gcggctgatc ctgctgggca
     2941 gcgtcgccgg cggcggcgac ccgtgcggtg tgactggcgc gctcgacgag cggatcgact
     3001 gtattgtgcc gttcaacttc ggcgggccgc agccggagaa tcgctacccc ctgccggccg
     3061 acgccgagac ctcgttcaac tacgctggcg gcggcagctg ggaatcgacg cggaatctct
     3121 atcgctcggc ggtggatggc ttcctgccct gggtgatcgt gggcagcgtc gccccgcgaa
     3181 agctgattca tgcccacgaa ttcagttggg accgcgagcg agatccggtc tggaagcggt
     3241 acgagaaaat ctacggcctg tatggcgcga aggacaatct cgccttcacc acgggcttcg
     3301 ggaccatcca aaacacgcag gccccggcct cgcactgcaa caacatcggt ccgccgcatc
     3361 gcaagctgat tcacgaagcg ctgggcaagt ggttcggcat caaagtcacg cccgaaagcg
     3421 aatacaagaa ccgccgcacg cgcgaggagc tgacctgcct caccgacgaa gcccgcaaac
     3481 aattccagcc caagctgctg catgagctgc tcgccaagat ggccgaccag caacttgcag
     3541 cggcccagaa atcgcgggcg gcaatgaaac cagacgagcg ccgcgaccag atgcgggacg
     3601 tctggacgcg gctgctgagc gacaccgagc cgccgaagga aatcaaagtc cgggacggct
     3661 cgccggcaac cgagcaggtc ggcggcgtca ccgtccgccg cgaattgctg gagactgcgc
     3721 ccggcatctc ggttccgctg atgacactct cgctcagccg cctcgacgac cggaaggatc
     3781 gcagccggtt cgcgattctg ggcctggcgg gcgacggatt gcaggccgcc ctgccgcgcc
     3841 gaaacaagga cattgccgag gggctgctcg ccggctgcgt gatcgcgctg gtcgatgtgc
     3901 gcggtctggg cgcggccggc tccgagcagg accgcggcca gcaaagcagc tcgacggggc
     3961 actcggcgac acagctcatg ctgggacaga cgatgctcgg cggccagctg cgcgatctgc
     4021 gggccgcctg gcagcatctg cagcgccgcg aggacgtgtc gcggaacgtg gtcatcgcgg
     4081 gcggcagcgg ccgggagccg ctcgcggccg acgcggaatt caagtatccg cggcgcgtcg
     4141 atcggccggc ggagagccag ccggaagccg cgctgctgtc gctgctcttc ggcttgtacg
     4201 agccggcctc gacggtgatc gtcagccgcc acggcctcgt ctcctaccgc tcgatcctgg
     4261 acagcccgtt cgtgcaagcg ccgcatgcag cgatcgtgcc gggcgcgttg cagctggtcg
     4321 acttacccga cctggcggta gtgctcgcac cgcgcgaggt tgtgctggac gtgctcgtgg
     4381 acggccgcgg gcgattgatt cccaaggcgg tggcggaagc aacgtacgag gcggcggccc
     4441 gggccaacac cggatcggag cggcccgcca gcctgcaaat cagcgaaacc gattcgccgc
     4501 tcgtgggccg ccagccatga agcgcagctc gccggccaat ccgcagatcg cggcgctgct
     4561 gcagctgctc gacgaagcct acgaaaagca ggcctggcac gggccgaacc tgcgcggctc
     4621 gctccgcggc atcgagccgg cccaggcggc gtggcggccg gccgcggggc ggaagaacat
     4681 ttgggaaatc gccctgcacg ccgcgtattg gaagtacgcc tgccgccgcc gcctggcggg
     4741 cgaaaagcgc ggctcgtttc cgctgaaggg aagcagttgg ttcgagcggg gcgacgccag
     4801 cgacgagcgg cagtgggtag gcgacctggc cctgctcgag cggatgcacc gcgaattgcg
     4861 ggcggtcgtc gccgcgtatc cgcccaccaa gctgctcgtc cggatcgccc cgaccaagca
     4921 cacgccgctg cggctgatcc agggagtcgc gctgcacgac gtctatcaca ccggacagat
     4981 ccagacgctc aaacggctct attcccagtc ttgagcgtgc tgcccgaggg gctgctagca
     5041 gcccgcgact ttgccggctg agcaagctct gccggtcgcg cccggcgaag tcatgcgcta
     5101 cgtccggcat attcgataca tccggtctag aaccacgcgc cgatattcgg ggcttgcagg
     5161 cggcgagccc tggcaatcgt caacctgcaa aggaagttgg ccatgagcct tgtccgtcgc
     5221 ccgctgctgg cagccgcact ctttgcgctg ctcttcccgg cgatcgcgcg ggcccagtcg
     5281 tccgagatca acgtcacggg aaagaaagcg gcgtattccc tctccgatga aaccggcctc
     5341 gtctcgcagc agccggacct ggtgccggag ccgccgccgc tgccgatagc gccgcagcgc
     5401 tcgcccagcg accagcccag cgtcctggcc ccgtcttaca acgtctcggg cggcgataag
     5461 tacacctgct gcgattcgtg cgactcctgc tgcggctgcg gctttttgag cagctggccc
     5521 tgcggctgcc cgctggaatg cctgggcgaa gcctgcaagc tttgggagcc ctgctgcgaa
     5581 gacagcccct ggagcggtgc ggggtggctg gccgcgggat tcgtctggaa cccgtacagc
     5641 ccggtcgatc gcttcaacgg cccgatgacc tggaatgacc gggccaatga
//
LOCUS       DASRJB010000795         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2492852, whole genome shotgun sequence.
ACCESSION   DASRJB010000795 DASRJB010000000
VERSION     DASRJB010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492852"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFB80_20950"
     CDS             <1..335
                     /locus_tag="VFB80_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36316.1"
                     /translation="VTFDGQAPPGPGSIYFLPQEAAEGFPSRPATGDFDKDGNYKVKT
                     FDPGDGLMPGKYTMHVECWQTPPNMEGKPVKSFVPAKYQSAASSGFSVEITTDMRSKE
                     VNLDIATK"
     gene            598..1851
                     /locus_tag="VFB80_20955"
     CDS             598..1851
                     /locus_tag="VFB80_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36317.1"
                     /translation="MEARLLRAALAAALALLFCGAARAQYPGQFASRGGPGASVRSQH
                     FIVTAPSAELANEICQAAERFRRDLAIEWLGHELPEWRDMCPIRVKLDPGAGGATSFV
                     FMGGMPTQWTMQIQGTRERLLDSVLPHEITHTIFATHFGQPLPRWADEGACTTVEHPS
                     EKAKQDQLLMRFLGPENRGISFNKMFAMRDYPRDILPLYSQGYSLTRFLIQHGGKRKF
                     VAYVGEGLQTNNWPAVTRKFYGFDDLSDLQVTWVAWVQQGSPNLENAASLAKAAPPAQ
                     PLTQMASLTQQPQSRIPDGALSSWQTPQSQNARALAQFDRGEPPLAESGLAAPEQPQP
                     AANVSSVSRPVSDGWYARRRDQAQAVRQATPAAATSGSGQMRLIPSPPPQTPTHPAAT
                     GRKVLMEWTRPQERMAADTVELARR"
     gene            complement(1907..>2299)
                     /locus_tag="VFB80_20960"
     CDS             complement(1907..>2299)
                     /locus_tag="VFB80_20960"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN36318.1"
                     /translation="ASLLFFFILIVQLNDVFQYVWSQLRGNRVIAPQINASRTWEGLI
                     GGVLSTTAVGMLLMWATPFGYWAGPIAGVVALAGFAGSMTMSGIKRDRNVQDYGTLVS
                     GHAGVLDRIDSLCFAAPVFFHITRYFFT"
BASE COUNT          428 a          765 c          771 g          335 t
ORIGIN      
        1 gtgtgacgtt cgacggccaa gccccgccgg ggccgggctc gatttatttc ctgccgcaag
       61 aggccgccga gggatttccc agccggcccg ccaccggcga cttcgataag gacggcaact
      121 acaaagtcaa gacgttcgat cccggcgacg gcctgatgcc gggcaagtac acgatgcacg
      181 tcgagtgctg gcagacgccg cccaacatgg agggcaagcc ggtcaaaagc ttcgttcccg
      241 cgaagtacca gtcggccgct tcgagcggct tcagcgtcga gatcacgacc gacatgcggt
      301 cgaaggaagt caacctcgac atcgccacga aatgagccgc ggcgaactgc tctggacgat
      361 tcgcagggcg tgggttaagg tttgcaaaac ttacaacggt ggtgcggacg attcgccagg
      421 agggcagcgg ccgcagcagc ggtaagtgaa tggccggccg cgacttaggg cagggcgctc
      481 tgggcggcat cagctgttcg gctcgcggtt tgcaatcccg ccgcaaacac gcgagtcatc
      541 ccgcggcagc cggaagaacc ggcagcctga ctcggtatcc agggaaggac ccacagcatg
      601 gaagctcgtc tgcttcgcgc ggccttggcg gcggcgctgg ctctgttgtt ctgcggcgca
      661 gcccgcgccc aatatcccgg acaatttgcc tcgcgcggcg ggccaggtgc tagcgtccgc
      721 tcgcagcact tcatcgtcac cgcgcccagc gccgaactgg ccaacgaaat ctgccaggct
      781 gccgagcgct ttcgccgcga cctggcgatc gagtggctcg gccacgaatt gccggagtgg
      841 cgcgacatgt gcccgatccg cgtgaagctc gatcccggcg cgggcggcgc gaccagcttc
      901 gtcttcatgg gcggcatgcc cacgcaatgg acgatgcaga tccagggcac gcgcgagcgg
      961 ctgctcgaca gcgtgctgcc gcacgagatc acgcatacga tttttgccac gcacttcggc
     1021 cagccgctgc cgcggtgggc cgacgaaggg gcctgcacga cggtcgagca ccccagtgaa
     1081 aaggccaagc aggaccagct gctcatgcgc tttttggggc cggagaaccg cggcatctcg
     1141 ttcaacaaga tgttcgcgat gcgcgattac ccgcgcgaca tcctgccgct ctattcgcag
     1201 ggctattcgc tgactcgctt tttgatccag cacgggggca agcgaaagtt cgtggcctac
     1261 gtcggcgaag gcctgcaaac caacaactgg cccgccgtca cgcggaagtt ctacggcttc
     1321 gacgacctct cggacttgca ggtgacctgg gtcgcttggg tccagcaagg cagtccaaac
     1381 ctggagaatg ccgcctcgct ggcgaaagcc gcgccgccgg cccagccgct gacgcagatg
     1441 gcctcgctca cgcagcagcc gcaatcgcgg attcccgacg gggcgctgtc gagctggcag
     1501 acgccgcagt cgcaaaacgc gcgggccctg gcccagttcg accgcggaga gccgccgctg
     1561 gcggagtcgg gcctggccgc gcccgagcaa ccgcagccgg cagcgaacgt cagcagcgtc
     1621 tcgcggccgg tgagcgacgg ctggtacgcc cgccgccgcg accaggcgca ggccgtccgg
     1681 caagccacgc cggccgcggc caccagcggc agcggccaga tgcggctgat cccgtcgccg
     1741 cctccccaaa cccccactca cccagccgcc accggccgca aagtcctgat ggaatggacg
     1801 cggccgcagg agcggatggc agcggacacg gtggaactcg cgcggcgcta gcatagtagg
     1861 catctcgctc cgcgagatgc acgcctcacg cggagcgtga ggactactaa gtaaagaaat
     1921 accgcgtgat gtggaagaag accggggcgg cgaagcagag ggagtcgatc cggtcgagga
     1981 cgccggcgtg gccgctgacg agcgtgccgt agtcctgcac gttgcggtcc cgcttgatac
     2041 ccgacatcgt catgctgccc gcgaagccgg ccagggccac cacgccggcg atcggcccgg
     2101 cccagtagcc gaacggcgtg gcccacatga gcagcatgcc cacggcggtc gtgctgagga
     2161 cgccgccgat cagtccctcc caggtgcggc tggcgttgat ctggggtgcg atgacgcggt
     2221 tgccgcgcaa ctggctccag acgtattgaa agacgtcgtt gagctggacg atcaggatga
     2281 agaagaagag caggctggc
//
LOCUS       DASRJB010000796         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_235351, whole genome shotgun sequence.
ACCESSION   DASRJB010000796 DASRJB010000000
VERSION     DASRJB010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235351"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFB80_20965"
     CDS             <1..399
                     /locus_tag="VFB80_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36319.1"
                     /translation="DAEAQRAPNMLLGGEVRMFHQHLSFAMYEAAERLEFDRAADYAL
                     AKVRYNTMLSRGGTTGHARMGDHFQEGHRWLEDNRARLSPAKSREVAEILERSTAELD
                     SAELLEQRDVIFADRSGGWSGRLRQILGDR"
     gene            complement(410..3109)
                     /locus_tag="VFB80_20970"
     CDS             complement(410..3109)
                     /locus_tag="VFB80_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZN36320.1"
                     /translation="MKLRVSLAALLSLAVTAGSLLGQASSPMSSGSPMSGGSGGSSPP
                     APPYASLLKDAKTASGMWTVHQKGNNLYWEIGSGDYGAEYIVLIAISRGIAQGQLLGG
                     MTWSTGDDWVWQFRKVGENVHIIRKNVRFKANANSPEARAVASAYTDSVLFSLPIMMK
                     GPKGGDLVDMTSVFMSDLPQIGQALPGFSFSSQKSTWAEDVKAFDENMELEVAATYAS
                     SGQAEIDTVPDSRGVTINVHYSISKVPNTGYSPRLADDRVGYFLTAVKDFSSKSDRDQ
                     FVRYINRWDLQKADSSVDPSPPKKAIIFHIEKTVPFKYRKAISDGIDEWNKAFEKAGF
                     VNAIEVTQQGDRDDKDPEDIRYNFFRWITSNAGFAMGPSRVNPYTGQILDADIIFDAD
                     FLTSWKQEYETLTARQIAQMTGGDLDLVGLAERPRTIFSDRPRSMDCQLSQGMSMQLA
                     FGQAAILAETDPKIAEANLEKLIQQGLKEVTMHEVGHTLGLRHNFKGSKWRSLSAMND
                     GGAGGCLVASVMDYSPTNIVPKGAKQGDYYSTTLGPYDFWAIEYGYKPLSGSTQGDAA
                     ELKKIAARSGDPALAYATDEDTRGTDPDPDTNRFDLGNDPLEYAGHRLQLVKELMPGL
                     VDRMTKEGEDFTQARRAFNVLIAQYGQSTFFVSRYVGGLSTSRSHKGDKDARPPISLV
                     EAGKQREALKLLEENMFSDKNFQFPPELLNSLGWSNWMHWGVQNTNRKDFGVHDFVLQ
                     WQERTLAQLLSSVTLKRMHDAELKAPTDADVMTTAELLSRLTKSIYSEVESTKEGDYS
                     NRKPAISSMRRNLQRSYLKELSDLALGVSNAPQDCQTVAYSELANLQQRIKSLLDNQP
                     VASKLDTYSRAHLQETGARIAKVLDARLNLPTPSPRIQIGPRS"
BASE COUNT          593 a         1143 c         1233 g          714 t
ORIGIN      
        1 gacgccgaag cgcaacgcgc gcccaacatg ctgctagggg gcgaggtgag aatgtttcat
       61 cagcatctca gtttcgccat gtacgaagcc gccgaacgac tcgaattcga tcgcgccgcg
      121 gactacgcgc tggccaaggt ccgatacaac acgatgctca gccgcggcgg cacgactggg
      181 cacgctcgga tgggcgacca cttccaagaa ggccaccggt ggctggagga caatcgcgcg
      241 cgtctctcgc ccgccaagtc gcgcgaggtc gctgaaatcc tggagcgttc caccgccgag
      301 ctcgactccg ccgagctgct ggagcagcgc gatgttatct tcgccgaccg ctccggtgga
      361 tggagcggac ggctgcggca aattcttggc gatcgctagg ctgcgtcggc tacgatcgcg
      421 ggccgatttg gatccgcggg gagggcgtgg gcaggttcag ccgggcgtcg aggaccttgg
      481 cgatccgggc gccggtctcc tgcaggtggg cccggctgta ggtgtcgagc ttgctggcca
      541 cgggctggtt gtcgagcagg ctcttgatcc gctgctggag gttggccagc tcggagtaag
      601 ccaccgtctg gcagtcctgc ggggcattgg agacgcccag cgcgaggtcg gacagctcct
      661 tcaggtacga ccgctgcagg ttccgccgca tgctgctgat cgctggcttg cggttgctgt
      721 agtcgccctc cttggtcgat tcgacctcgg aatagatcga cttggtcagc cgcgagagca
      781 attcggcggt ggtcatcacg tcggcgtcgg ttggcgcctt cagctcggcg tcgtgcatcc
      841 gcttgagcgt gaccgagctg agcagctggg ccagcgtccg ctcctgccac tgcagaacga
      901 agtcgtggac gccgaaatcc ttgcggttgg tgttctgcac gccccagtgc atccagttcg
      961 accagccgag cgagttgagc agctcgggcg gaaactggaa gttcttgtcg ctgaacatgt
     1021 tctcttcgag cagcttcagg gcctcgcgct gcttgccggc ctcgaccagc gagatcggcg
     1081 gccgggcgtc cttgtcgccc ttgtggctgc gcgaggtcga gaggccgccg acatagcgcg
     1141 agacgaaaaa cgtgctctgg ccgtactggg cgatgagcac gttgaacgcc cggcgggcct
     1201 gcgtgaagtc ctcgccttcc ttcgtcatcc gatcgacgag gcccggcatc agctccttca
     1261 cgagctgcag ccggtggccg gcgtattcca gcggatcgtt gcccaggtcg aagcggttgg
     1321 tgtccggatc gggatcggtt ccgcgggtgt cttcatcggt ggcataggcc agggccgggt
     1381 cgccgctccg cgcggcgatc ttcttcagct cggcggcgtc gccctgcgtg ctgccgctga
     1441 gcggcttgta gccgtactcg atggcccaga agtcgtaggg cccgagggtc gtgctgtaat
     1501 agtcgccctg cttggccccc ttgggcacga tattcgtcgg gctgtagtcc atcaccgagg
     1561 ccaccaggca gccgcctgcc ccgccgtcgt tcatcgccga gaggctccgc cacttgctgc
     1621 ccttgaagtt gtgccgcagg ccgagcgtgt ggccgacctc gtgcatcgtg acttccttga
     1681 gcccctgctg gatcagcttc tccaggttgg cctcggcgat cttgggatcg gtctcggcca
     1741 ggatcgccgc ctgcccaaag gccagctgca tgctcatccc ctgcgagagc tggcaatcca
     1801 tgctccgcgg ccggtcggag aagatcgtcc gcgggcgctc ggccaggccg accaggtcca
     1861 ggtcgccgcc ggtcatctgg gcgatctggc gggcggtgag cgtctcgtac tcctgcttcc
     1921 aggaggtgag aaaatccgcg tcgaagatga tgtcggcgtc gagaatctgc cccgtgtagg
     1981 ggttcacgcg gctggggccc atcgcgaagc cggcgttgct ggtgatccag cggaagaagt
     2041 tgtagcggat gtcctccggg tccttgtcgt cgcggtcgcc ctgctgcgtg acttcgatcg
     2101 cgttgacgaa gccggccttc tcgaaggcct tgttccattc atcgatgccg tcggagatcg
     2161 ccttgcgata cttgaacggc acggtcttct cgatgtggaa gatgatcgcc ttcttgggcg
     2221 gcgagggatc gaccgaagag tcggccttct gcaggtccca gcggttgatg taccgcacga
     2281 actggtcgcg gtcgctcttg ctcgagaagt ccttgaccgc cgtcaggaaa tagcccacgc
     2341 ggtcgtcggc cagccgcggc gagtagcccg tattggggac cttgctgatc gagtagtgga
     2401 cgttgatcgt cacgccgcgg gaatcgggga cggtgtcgat ctcggcctgg ccgctggagg
     2461 cataggtcgc ggcgacttcc agctccatgt tctcgtcaaa ggccttcacg tcctcggccc
     2521 aggtcgattt ctgcgacgag aagctgaacc ccggcagggc ctggccgatc tgcggcaggt
     2581 cgctcatgaa gaccgaggtc atatcgacca ggtcgccccc cttggggcct ttcatcatga
     2641 tcggcaggct gaacagcacg ctgtcggtgt aggccgaggc taccgcccgc gcttcgggcg
     2701 agttggcgtt ggccttgaac cgcacgttct tgcggatgat gtgcacgttc tcgccgactt
     2761 tgcggaactg ccacacccag tcgtcccccg tgctccaggt catcccgccc agaagctgcc
     2821 cctgggcgat gccgcggctg atggcgatca gcacgatgta ctccgcacca tagtcgcccg
     2881 agccgatttc ccagtagagg ttgttgccct tctggtgcac ggtccacatg ccgctcgcgg
     2941 tcttggcatc cttcagcagc gaggcatagg gaggggcggg cggcgagctg ccgccggagc
     3001 cgccgctcat gggcgaaccg ctggacatcg gcgaggaggc ttggcccagc agcgagccgg
     3061 cggttactgc gagggagaga agggcggcga gggagacgcg cagtttcatt cgagggtcct
     3121 gcaaaaagat ctggtcaacc ggcgagtggc gtcgccggaa cggttcaacc gaggcgggaa
     3181 ctggggcggg gagcggtggc aagccgtgcc aaggcccggt ttcaagctcg ttaaatatac
     3241 cccattctgc cgccctcacc acaatccgca gcggttttga cgaatctgca aaagttgcgg
     3301 actggtcatt tttctacggt cagcgagtca gtttggatcg aagcccaagt ccgtggcgcg
     3361 acacgaagca agtcattgtg accccccggt caggtaagcc gcaactgctc tgtagcgtcg
     3421 aaattcgacg caagctattg acttgtcggc ggttgcgtcc accctcccga aaaccgctta
     3481 acaactactt attagccttt gaggaccgga tccagcgggt ggcttccagg ccaaactggc
     3541 agcctccttg ccaactggaa ctagagagca aatagcgtac caaagttcac tgccgcggct
     3601 acggcgaggg gccgaggcgg aagtcgccgg ctggccccgc ccctgcgccg gccgggttca
     3661 gcgggttatt ggggtcggcg ggc
//
LOCUS       DASRJB010000797         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1925145, whole genome shotgun sequence.
ACCESSION   DASRJB010000797 DASRJB010000000
VERSION     DASRJB010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925145"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..784)
                     /locus_tag="VFB80_20975"
     CDS             complement(50..784)
                     /locus_tag="VFB80_20975"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HZN36321.1"
                     /translation="MVLQKIAREIMRPFRRRPVVARRQQPVVMEQAAPSQPAAPAAEA
                     FFADLVRKTDNFGRITWLGQPIWQNVLDLWTIQETIAEIRPELLIECGTNRGGSSLFF
                     AHLMDLLGQGLIITIDVEKMHKLSHPRITYLLGSSTAPEIVAEVQARASGTRGPVLVI
                     LDSDHSEAHVAAELAVYAPLVTRGSYCLVQDGVIDTLPMMAGARPGPLPAIDQFVAAH
                     PEFEIDHERCERFIVSHHPRGWLRRK"
     gene            complement(947..2242)
                     /locus_tag="VFB80_20980"
     CDS             complement(947..2242)
                     /locus_tag="VFB80_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36322.1"
                     /translation="MSHTVHIQSSVTIRRGLVQRRDFLKAIPAAAAAAGLHSWPDLMS
                     VRAADLRKSGKACILLWMQGAPSQFETFDPKPGHANGGETKAIKTAVSGIEIAENLPE
                     VAKTMRELCVIRSLNSREGQHPRATYLMHTGYLPTAAVKYPTIGSIVAKEIGNPASDL
                     PSFVRIGNSRFTDGAGLLGVDYDPLVMQAANRPPENTSLPSSAPRFQRRLSLLEQMQA
                     DYANHGGKQAVADHQKVYAQAAKLMLSPKMQTFDIGQEPQQARDAYGSSAFGQGCLLA
                     RRLIETGVTFVEVSLGNWDTHQDNFNRTRTLCQQLDQPFAALIADLKQRGMLDSTLIV
                     WMGEFGRTPRINPNSGRDHYPRAFSAVLAGCGVKGGQVVGATDAGGESVKGKPVGVSD
                     MLRTICHALGIDASKENMSSIGRPIKIVDGGEIVSQVFA"
     gene            complement(2357..>3746)
                     /locus_tag="VFB80_20985"
     CDS             complement(2357..>3746)
                     /locus_tag="VFB80_20985"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN36323.1"
                     /translation="AADKRAKIVEKLLASPQFGENWGRYWRDVIMYRKSEERAQIVSG
                     ALESFLTESFNANKPWSEIATAFITASGDARENGACGLIMAQQGQPEETVAEVSRIFL
                     GVQIQCAQCHDHPTDRWKREQFHQLAAFFPRVSARLILQPDRQDISVAVTDSPPRFMP
                     KGPMAMRRVGTPEHYMTDPKNPQSRGTLMQPVFFATGEKLPLGTRDAERRGSLAKWTT
                     AKDNPFFAKAFVNRLWAELTGEGFYEPIDDIGPDRTCTARETLDYLAGQFAESGYDVK
                     WLFRTIMASELYQLPSSSRRGPEDPPMQHNVAQRLRGDQLFDNLLVALEASEPQSFAP
                     GGPGGLGALYGRGRGPRQQFNTAFGYDPSERRDEIAHSIPQALTMMNSPLIGQALRGS
                     GRTMLARLLGEIQDDKALTQELYLRVLGRQPSESELATAARYVKIVGSRTEAFEDLLW
                     GLVNSAEFLHRT"
BASE COUNT          576 a         1234 c         1249 g          687 t
ORIGIN      
        1 attcggattt ctcgcggtcc gaattctggc gaattcggct accaagtgct cacttccgcc
       61 gcagccagcc gcgggggtga tgcgagacga tgaatcgctc gcagcgctcg tggtcgatct
      121 cgaactctgg gtgagcggcg acaaactggt cgatcgccgg cagcgggccg gggcgagcgc
      181 cggccatcat cggcagcgtg tcgatcacgc cgtcctgcac gaggcagtag ctgccccgcg
      241 tgacgagcgg tgcgtacacg gccagttccg ctgcgacgtg cgcctccgaa tggtcgctgt
      301 cgaggatgac gagcacggga ccgcgtgttc cgctggcccg cgcctgcact tccgcgacga
      361 tctccggcgc ggtcgagctg cccagtagat acgtgattcg cggatgcgag agcttgtgca
      421 tcttttccac gtcgatcgtg atgatcaggc cctgtcccag caggtccatc aggtgggcga
      481 aaaacagcga cgagccgccg cggttcgtgc cgcactcgat cagcagttcg gggcgaattt
      541 cggcgatcgt ctcctggatc gtccacaggt ccagcacgtt ctgccagatc ggctggccca
      601 gccaggtaat tcgtccgaaa ttgtcggtct tgcgaacgag gtccgcgaaa aatgcctcgg
      661 ccgccggtgc cgccggctgc gaaggggccg cctgctccat caccaccggc tgctgccgcc
      721 gagcgacgac cggccgccgc cggaacggcc gcatgatctc gcgggcaatc ttctgcaata
      781 ccatgtttcc ctcctcacgg cgcactccgc cgatcgtgag gggagggaaa tagcagacat
      841 tctcaacagc ggcaagggcg aatctggatt gttcgtttaa cccgtagccg gaggcgcggg
      901 cttttcgcgg gtagccgccg tcgcctccgg ctccgggtta aacgcgctac gcgaatacct
      961 gggagacaat ctcgccgcca tcgacgatct tgatcggccg gccgatgctg ctcatgttct
     1021 ccttgctggc gtcgatgccc agggcgtggc agatcgtgcg gagcatgtcg ctgacgccaa
     1081 ccggcttgcc cttgacgctc tcgccgccgg cgtcggtcgc gccgaccacc tggccgccct
     1141 tcacgccgca gccggcgagc accgcgctga acgcccgcgg gtagtggtcg cggccgctgt
     1201 tggggttgat ccgcggcgtg cggccgaact cgcccatcca gacaatcagc gtgctatcga
     1261 gcattccgcg ctgcttgagg tcggcgatca gcgcggcaaa gggctgatcg agctgctggc
     1321 agagcgtccg tgtgcggttg aagttgtcct ggtgcgtgtc ccagttcccg aggctgacct
     1381 cgacgaacgt cacgccggtc tcgatcagcc ggcgagccaa caggcacccc tgcccgaagg
     1441 ccgagctgcc gtaggcgtcg cgcgcctgct gcggctcctg gccgatgtcg aaggtctgca
     1501 tcttcggact gagcatcagc ttcgcagcct gggcgtagac cttttgatgg tcggcgacgg
     1561 cctgcttgcc gccgtggttg gcatagtcgg cctgcatctg ctcgaggagc gagagccgcc
     1621 gctggaatcg cggcgcggac gatggcaggc tcgtgttttc cggcgggcgg ttggcggcct
     1681 gcatcaccag cggatcgtaa tcgacgccga gcaagcccgc tccgtccgtg aacctggaat
     1741 tgccgattcg cacgaagctc ggcaggtcgc tggccggatt gccgatctcc ttggcgacga
     1801 tcgagccgat cgtcggatac ttcaccgccg ccgtcggcag gtagccggtg tgcatcaggt
     1861 aagtcgcccg cggatgctgc ccttctcggc tgttcagcga gcggatgaca cacagctcgc
     1921 gcatggtctt ggcgacttcc ggcaggttct cggcgatctc gatgccactg acggcggtct
     1981 tgatcgcctt ggtctcgccg ccgttggcgt ggcccggctt gggatcaaac gtctcgaact
     2041 ggctcggcgc gccttgcatc cagagcagga tgcaggcctt cccgctcttg cgcaagtcgg
     2101 cggcccgcac gctcatcagg tcgggccacg agtgcaagcc ggccgcagcc gcagccgccg
     2161 ggatcgcttt caggaaatcg cgccgctgta ccagcccgcg gcgaatcgtg acgctggatt
     2221 ggatgtggac ggtgtgggac ataggagccg gtgggtcggt tgtagggtgg gacaaggccg
     2281 ccgcggccgc cggcccacca ttggtcgata aagcaggtgg gccggcgctc gcggactcgc
     2341 tggtcccacc ctacgtctag gtccggtgca aaaactccgc ggaattcacg aggccccaga
     2401 gcaggtcttc aaaggcctcg gtccgggagc cgacgatctt cacgtaccga gctgctgtcg
     2461 ccagctcgct ttcgctcggc tggcggccga gcacgcggag atacagctcc tgcgtcaggg
     2521 ccttgtcgtc ttggatttcg cccagcaggc gggccagcat cgtgcgtccg ctgccgcgaa
     2581 gggcctggcc gatcagcggc gagttcatca tcgtcagcgc ctgcggaatc gagtgggcga
     2641 tctcgtcgcg ccgctcgctg gggtcgtagc cgaaagccgt gttgaactgc tgccgcggcc
     2701 cgcggccccg gccgtacagt gcacccaagc cgcccgggcc gcccggcgca aacgactgcg
     2761 gctcgctggc ctccagtgcg accaggagat tgtcgaacag ttgatcgccc cgcaatcgct
     2821 gggcgacgtt gtgctgcatc ggcggatctt ccggcccgcg gcggctgctg gagggcagct
     2881 gatacagctc gctcgccatg atcgtgcgga agagccattt gacgtcgtag ccgctctcgg
     2941 cgaactggcc cgccagatag tcgagcgtct cgcgcgctgt gcaggtacga tccgggccga
     3001 tgtcgtcgat cggctcatag aagccctcgc cggtcagctc cgcccacaag cggttgacga
     3061 aggccttggc aaaaaacggg ttgtccttgg ccgtcgtcca tttcgccagg ctgccgcggc
     3121 gctcggcgtc gcgcgtgccc agcggcagct tctcgccggt cgcgaaaaac accggctgca
     3181 tcagcgtgcc ccgcgactgc ggattcttgg ggtccgtcat gtaatgctcg ggcgtgccga
     3241 ctcggcgcat cgccatcggc cccttgggca taaaccgcgg cgggctgtcc gtgacggcca
     3301 ccgaaatgtc ctggcgatcc ggctggagaa tcagccgggc cgagacccgc gggaagaacg
     3361 cggcgagctg atgaaactgc tcgcgcttcc agcgatccgt cggatggtcg tggcactggg
     3421 cgcactgaat ctgcacgccc aaaaagatgc ggctgacctc ggccacggtt tcttcgggct
     3481 gcccttgctg ggccatgatc aggccgcagg ccccgttctc gcgggcgtcg ccgctggcgg
     3541 tgatgaaggc ggtggcgatc tcgctccagg gtttgttggc gttgaagctc tcggtcagga
     3601 agctctccag agcgccgctg acgatctggg cccgctcctc cgacttgcgg tacatgatca
     3661 cgtcgcgcca atagcgaccc cagttctcgc cgaattgcgg gctggccaag agcttctcga
     3721 caatcttcgc ccgtttgtcg gctgcc
//
LOCUS       DASRJB010000798         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2494053, whole genome shotgun sequence.
ACCESSION   DASRJB010000798 DASRJB010000000
VERSION     DASRJB010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494053"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFB80_20990"
     CDS             complement(<1..906)
                     /locus_tag="VFB80_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36324.1"
                     /translation="MPRADLLALTPDDLAAITNRGTVKRAERELEEGTLTCTLTENAG
                     ELVAEWSDGIVCRFPAGQTMHEATCTSGIAGISRHVVRSVLAYQRQCRAPVDDAASVV
                     TATSADWDPGTITDEALAARFRQAAVAKAAQRFAQGVLVELIRGAKPVARFLDTPCTL
                     RFLVPGDLRYITADCDESLLATFVPMAVWAFRQLPPNQAAGLVAHQQADLATPSDLLA
                     DLNALFAELGVSGIAGCGPTWPQRLTRLENSLRDAGLIWPAELAADLFEQHGMYQAHD
                     ARFDPLEVVALVGELVARGRAIAGGT"
     gene            complement(909..2048)
                     /locus_tag="VFB80_20995"
     CDS             complement(909..2048)
                     /locus_tag="VFB80_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN36325.1"
                     /translation="MSQEQHQQRLARWRLVMGKGAEQLCGGLSGEDATRDACLGYLYD
                     REYGSGRNVRGADGNQGGLGESNLTVPEWINQVHELFPKRTIERLEKDALERYQLDEM
                     VTNAEVLSRAQPNPTLLKAVLRTKHLMNQDVLKAAQHLARQVVEELMKKLSQEVKNPF
                     LGALDRRRRSHLKIAKNLDLKTTLRRNLKHYDPERRRLVIESPIFFSRVRRHVDRWQI
                     IILVDESGSMLDSVIHSAITASIFFSLRTMRVHLCLFDTQVVDVTDQCTDPVETLMKV
                     QLGGGTDIGQALSYASDLVDNPRRTIVVLITDFFEGAPLDRLYAAAKRLIDSGVTLLG
                     LAALDERAEPTYDRNVAAHLVQMGAHVAAMTPGELAAWVAEKVRR"
     gene            complement(2045..>4222)
                     /locus_tag="VFB80_21000"
     CDS             complement(2045..>4222)
                     /locus_tag="VFB80_21000"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HZN36326.1"
                     /translation="LLLDHELPIAIYSYFQDAAGERRGAYYPFSEYSPEWQAIRRAAE
                     LEIPTRFIDLPWVEVAHLDRATHRYADAELRRGRYVRALCDRLGVEDFDDLWDKLIES
                     EERLTLETYLQRVHLLCWHTRLWEPEVSLADQRREAFMAQQIRAAEEQHDGPILVVTG
                     GFHSSALAARLEGFRDCPGTGELPQTPEQTPGNAEQSSEIQDRGIALTSYSYERLDNL
                     TGYDAGLPSPGFYEHVWQTQRRGEAFGHQPLLRQVVQELRERKQRISTADLIAVETSA
                     RALAAIRGRERVWRRDLIDAATSALVKDELEYGVRSPLVDAVHAVLRGKRRGRLAAGA
                     RLPPLVVDLQRQLQAADLELTRGVRTVELNLLDADDRTKSRLLHSLSVLGIAGYQRTA
                     GTDFLARQDLARLWEEWRLAWSPEFEATSIEAARYGTSLADAASARLSELAAADERSA
                     AKAAALLVQAAQAGVQAISTPLLARLEGLIQNEAEFTAAAAALGHLLYLYCHDEAFGT
                     AHLPRLGKLLAETFTRSLWLLESLGQSTTPARELLAGLKTLLEAHERAGDVLAASDGE
                     LAAVLSRVEADPRKPPQLRGAAVGILWTIGQASPDRVLSQLMLFSQPDQLGDFLTGLF
                     ALAREVAQRHPQLVQAIDRALLEFAPEQFHETLPSLRLAFTYFTPREKHYMLTTLFQA
                     LGFKQVQPLAALAVDEQTAAAALALEERIFAAVARYGLEGRAP"
BASE COUNT          649 a         1396 c         1484 g          693 t
ORIGIN      
        1 cgtcccaccg gcgatcgccc gcccgcgcgc gactagctcg ccgaccagcg cgacgacctc
       61 tagcggatcg aaacgggcgt cgtgggcctg gtacatgccg tgttgctcga atagatccgc
      121 ggccaactcg gcgggccaga tcagcccggc gtcgcgcagc gagttttcca ggcgcgtcag
      181 ccgctggggc caggtcggcc cgcagccggc gattcccgaa acgccaagtt ccgcgaatag
      241 cgcattgagg tcggcgagca gatcggatgg cgtcgcgaga tcggcctgct ggtgcgcgac
      301 gaggccggcc gcctggttcg ggggcagctg ccggaaggcc cagacggcca tcggcacgaa
      361 cgtggccagc agcgactcgt cgcaatcggc ggtgatatag cgcagatcgc ccggcacgag
      421 gaaccgcagc gtgcagggcg tatcgagaaa gcgggccact ggcttggccc cgcggatcag
      481 ctcgacgagc acgccctgcg caaatcgctg agcggccttg gccacggctg cctggcgaaa
      541 acgggccgcc aaggcttcgt cggtgatcgt gccgggatcc caatccgccg atgtcgcagt
      601 gacgacgctg gcagcgtcgt caacgggggc gcggcattgt cgctggtagg ccagcaccga
      661 gcggacgacg tgccgactga tgccggcgat cccgctggtg caggtggcct cgtgcatcgt
      721 ctggcccgcg ggaaagcggc agacgatgcc gtcgctccac tcggcgacga gctcgccggc
      781 gttttccgtg agcgtgcagg tgagtgtgcc ttcctccagc tcgcgctcgg cccgcttgac
      841 ggtgccgcga ttggtgatcg ccgccaggtc gtcgggggtc aaagcgagaa ggtcggcgcg
      901 gggcatgctt acctgcggac tttctcggcg acccaggcgg ccaattcgcc gggcgtcatc
      961 gcggcgacat gcgcgcccat ctgcaccagg tgggcggcga cgttccggtc gtacgtcggc
     1021 tcggcccgct cgtcaagcgc ggccaggccc agcagcgtca cgccgctgtc gatcagccgc
     1081 ttggccgcgg cgtacagccg gtcgagcggg gcgccttcga agaaatcggt gatcagcacg
     1141 acgatcgtcc gccgcgggtt atcgaccagg tcgctcgcgt agctgagggc ctggccgatg
     1201 tcggtgccgc cgccgagctg gaccttcatc agcgtctcga ccggatcggt gcactggtcg
     1261 gtgacatcga cgacctgcgt gtcgaacagg cagaggtgga cccgcatcgt ccgcaggctg
     1321 aagaagatcg aggcggtgat cgccgagtgg atcacgctgt cgagcatgct ccccgattca
     1381 tcgaccagga tgatgatctg ccagcgatcg acgtgccgcc gcacgcggga gaagaagatg
     1441 ggcgattcga tcaccagccg gcggcgctcg gggtcgtagt gcttgaggtt ccggcggagc
     1501 gtggttttca gatcgagatt cttggcgatc ttgaggtgcg agcggcggcg gcggtcgagg
     1561 gcgccgagga acggattttt cacctcttgc gagagcttct tcatcagctc ctcgaccacc
     1621 tggcgggcca ggtgctgggc cgccttcaag acgtcctggt tcatcaggtg cttggtccgc
     1681 agcaccgcct tgagcagcgt cgggttcggc tgcgcccggc tgaggacttc cgcattggtt
     1741 accatctcgt cgagctgata ccgctccagg gcgtcttttt ccagacgctc gatcgtccgc
     1801 ttgggaaaca gctcgtggac ctggttgatc cactcgggaa ccgtcaggtt cgattcgccg
     1861 aggccgccct ggttgccgtc cgctccgcgg acgttccgcc cgctgccgta ttcgcggtcg
     1921 tagagatagc ccaggcaggc gtcgcgggtg gcgtcttcgc cggacaggcc gccgcagagc
     1981 tgctcggccc ccttgcccat gaccagccgc cagcgggcga gccgctgctg atgctgctct
     2041 tggctcatgg cgccctccct tccaggccgt agcgggcgac ggcggcaaaa atccgctctt
     2101 ccagcgccag ggccgccgcg gccgtctgct catcgaccgc cagcgccgcc agcggctgca
     2161 cttgcttgaa tcccagagcc tgaaacagcg tcgtgagcat gtagtgcttt tcccgcgggg
     2221 tgaagtaagt gaacgccagc cgcagcgagg ggagcgtctc gtgaaactgc tcgggagcga
     2281 attcgagcag ggcccggtcg atcgcttgca ccagctgagg atgccgctgg gcgacctcac
     2341 gggccagagc gaacagcccg gtgagaaagt cgcccaactg atcgggctgc gagaagagca
     2401 ttagctggct gaggacgcga tcgggcgagg cctggccgat cgtccaaagt atgccgaccg
     2461 cggccccgcg gagctgtggc ggcttgcgtg ggtcggcctc gacgcggctg agcacggctg
     2521 ccagctcgcc gtcgcttgcg gccagcacgt caccggcccg ctcgtgagcc tcgagcagcg
     2581 tcttcaggcc ggcgagcagc tcgcgcgcgg gggtggtcga ttggccgagc gattcgagca
     2641 gccagagcga gcgagtgaac gtctcggcca gcagtttgcc cagccgcggg aggtgtgccg
     2701 tgccgaacgc ctcgtcgtga caatacaagt aaagcaggtg gccaagggcc gccgcggccg
     2761 cggtgaattc ggcctcgttc tgaatcaacc cttcgaggcg ggccaagagc ggcgtcgaaa
     2821 tcgcctgaac accggcctgt gctgcctgca cgagcaatgc ggccgccttg gcggcgctcc
     2881 gctcgtcggc cgcggccagc tccgacagcc gggcgctggc ggcgtcggcc agggacgtgc
     2941 cgtagcgagc cgcttcgatc gacgtggctt cgaattcggg actccaggcg agccgccact
     3001 cttcccacag gcgggccaaa tcctggcggg ccagaaagtc ggttcccgcg gtgcgctggt
     3061 agcccgcgat gcccagcacg gacaggctgt gcagcaggcg gctcttggtg cggtcatcgg
     3121 catccagcag gttcagctcg acggtccgca cgccgcgcgt cagctccagg tcggccgcct
     3181 gcaattgtcg ctgcaaatcg acgaccagcg gcggcaggcg tgcgcccgcg gcgagtcggc
     3241 cgcggcgctt gccgcggagg accgcgtgca cagcatccac gagcggcgag cggacgccgt
     3301 actccagctc gtccttcacc agggcgctgg tggccgcgtc gatcaggtcg cgccgccaga
     3361 ctcgctcgcg gccgcggatg gcggccaggg cgcgggcgga agtctccacc gcgatcaggt
     3421 cggccgtgct gatccgctgc ttgcgttccc gcaattcctg gacgacttgc cgcagcagcg
     3481 gttgatgccc gaacgcttcg ccgcgacgct gcgtctgcca gacgtgctcg tagaaacccg
     3541 ggctgggcag gccggcgtcg tagccggtga ggttgtccag tcgctcgtag gaatagctcg
     3601 tcagggcgat gccgcggtcc tggatttcgc tgctctgctc ggcgttccct ggcgtctgct
     3661 ccggcgtctg cggcagctca cctgtgccgg ggcagtcgcg aaagccttcc agccgggccg
     3721 cgagcgccga gctgtgaaag ccgccggtca cgaccaggat tgggccgtcg tgctgttcct
     3781 cggcagctcg aatctgctgg gccatgaagg cctcgcggcg ctggtcggcg aggctgacct
     3841 ctggctccca caggcgcgtg tgccagcaga gcaaatggac gcgctgcaga taggtctcca
     3901 gcgtgagccg ctcttcggat tcgatcagct tgtcccacag gtcgtcgaag tcttccacgc
     3961 ccagccggtc gcagagcgcc cggacatagc gcccgcggcg gagctcggcg tcggcataac
     4021 ggtgcgtggc ccggtcgaga tgggcgactt ccacccaggg aagatcgata aaccgtgtcg
     4081 gaatttccag ctcggcggct cggcggatcg cctgccactc gggcgagtac tcggagaacg
     4141 gatagtaagc cccgcgccgc tcgcccgctg cgtcctggaa atagctgtag atcgcgatcg
     4201 gcagctcgtg gtccagcagc ag
//
LOCUS       DASRJB010000799         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_524183, whole genome shotgun sequence.
ACCESSION   DASRJB010000799 DASRJB010000000
VERSION     DASRJB010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524183"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFB80_21005"
     CDS             complement(<1..397)
                     /locus_tag="VFB80_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN36327.1"
                     /translation="MKKSHVITTVNGETAEFLCEPRQSLLEVLRDTLRLTGAKEGCNN
                     GNCGACTVLMNGLPVNSCLVLAVEAEGAQVETVESLAQQGHLHPLQQCFLEGAALQCG
                     VCTPGFLVAAKALLDKNPSPNEHEIRFNLA"
     gene            complement(456..1334)
                     /locus_tag="VFB80_21010"
     CDS             complement(456..1334)
                     /locus_tag="VFB80_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN36328.1"
                     /translation="MKDFSYSAPATLAEATRLLAGANGKAMILAGGTDIIVQLREGLR
                     EADVVVDVKKIPELAELSYSPAKGLRLGASVPCYRIYEDAAIAAAYPALADAARIVGG
                     WQIQTRASVGGNLCNSSPAADTIPALIAHGATCVIAGPKGERQVKVEEFCTAPGRNVL
                     QPGELLVALVFLPPAKSAGSAYERFIPRNEMDIAVVGAGSWVQLAKGGEEIEKARIGL
                     AAIAPTPRFAAEASQWLTGQPATEDIFARAGELARNVAAPISDMRGTAEYRMHLIGVL
                     VKRTLLKAAERARAGG"
     gene            complement(1482..2051)
                     /gene="dcd"
                     /locus_tag="VFB80_21015"
     CDS             complement(1482..2051)
                     /gene="dcd"
                     /locus_tag="VFB80_21015"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZN36329.1"
                     /translation="MILSGDEIVRNLGTNIVIEPFDDTRLNPNSYNLTLHDELLTYEE
                     VVLDMAEPNRTRRLRIPKEGLVLTPQKLYLGRTAERTETHNFVPMIEGRSSIGRLGLF
                     VHVTAGFGDVGFCGYWTLEMFAIQPIRIYPGVPICQIFYHQVAGSITEYCSDKYQHNK
                     DIQPSLFYKELNPQSERPDPQKVFVFGEK"
BASE COUNT          355 a          663 c          718 g          400 t
ORIGIN      
        1 tggcgaggtt gaagcggatt tcgtgttcgt tggggctcgg gttcttgtcg agcagggcct
       61 tggcggcgac gaggaagccg ggggtgcaga cgccgcattg cagcgcggcg ccttccagga
      121 agcactgctg gagcggatgc aggtggccct gctgagcgag gctctcgacg gtttcgacct
      181 gcgccccctc ggcctcgacg gcgaggacca ggcagctatt caccggcagg ccgttcatca
      241 gcacggtgca agcaccgcaa ttgccgttgt tgcagccttc cttcgccccg gtcaggcgga
      301 gcgtgtcgcg aagcacctcc agcaggcttt gccgcggctc gcagaggaac tcggcggttt
      361 cgccgttgac ggtcgtgatg acgtgggatt ttttcacgga gtagtgaggg gtcggtggtg
      421 agtggtgagt ggtgtgaacc cgcggctccc tagccctagc ccccagccct agccctttcc
      481 gcggccttca gcagcgtccg tttgacgagg acgccgatca ggtgcatgcg gtattcggcg
      541 gtcccgcgca tgtcgctgat gggagcggcg acattgcggg ccagctcgcc tgcgcgggcg
      601 aagatgtctt ccgtcgcggg ctgtccggtg agccactgtg aggcttcggc ggcgaaccgc
      661 ggcgtcggcg cgatggcagc caggccgatg cgggcctttt caatctcttc gccgccctta
      721 gcaagctgca cccacgagcc cgcgccgacc acggcgatat ccatctcgtt ccgcgggatg
      781 aatcgctcgt aggccgagcc ggcgcttttg gctggcggca gaaagaccag cgccaccagc
      841 agttcgcccg gctgcagcac gttgcgaccc ggcgcggtac agaactcctc gaccttcacc
      901 tgccgctcgc ccttgggccc ggcgatgacg cacgtcgcac cgtgggcgat cagggcggga
      961 atagtgtcgg ccgccggcga cgagttgcac aagttgccgc cgacgctggc ccgcgtctga
     1021 atctgccagc cgccgacgat ccgtgccgcg tcggccagcg cgggataggc cgcggcaata
     1081 gcagcgtcct cataaatgcg gtaacagggc acgctcgccc ccagccgcag tcccttggcc
     1141 ggtgaatagc tcagctcggc cagctccggg atcttcttga catcgaccac gacgtccgct
     1201 tcacgcaggc cttcgcggag ctgcacgatg atgtcggtcc cgcccgcgag aatcatggcc
     1261 ttgccgttag caccggccag cagccgcgtg gcctcggcga gggttgccgg tgcggaatag
     1321 gaaaaatcct tcaagttcac ctcggcggtc gatgatggcg ggattcagtg agaggattat
     1381 agcgggatgc cggagcggct tcgacggccg ggaagcgggg cttccagacg tgcgtgccca
     1441 agcagagctt gggcacgaga acctacctca ccgcctatca ctcacttttc gccaaacaca
     1501 aacaccttct gcggatccgg ccgctcggac tgcgggttga gctctttgta gaacaagctg
     1561 ggctggatgt ccttgttgtg ctggtacttg tcgctgcaat attcggtgat gctgccggcg
     1621 acctggtgat agaagatctg gcagatcggc acgccgggat aaatgcggat cggctgaatg
     1681 gcgaacatct ccagcgtcca gtagccgcag aagcccacgt cgccgaagcc ggccgtgacg
     1741 tgcacgaaca ggcccagccg gccgatgctc gagcggccct cgatcatcgg cacgaagttg
     1801 tgggtttccg tacgctcggc cgtgcggccg agatagagtt tctgcggcgt gagaaccagg
     1861 ccctccttgg gaattcgcag ccgtcgcgtg cggttcggct cggccatgtc gaggacgact
     1921 tcctcgtacg tcagcaactc gtcgtgcagc gtgaggttgt agctgttcgg gttgagccgc
     1981 gtgtcgtcga acggctcgat gacgatattg gtgccgagat tacgaacgat ttcgtcgccg
     2041 gagaggatca tcgaaacacc tgccggtgag aaaggcccag ccgcgcgagt tcagacacgc
     2101 ggcgttattg taccgattcc gcctccgatg tgggcg
//
LOCUS       DASRJB010000800         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3339964, whole genome shotgun sequence.
ACCESSION   DASRJB010000800 DASRJB010000000
VERSION     DASRJB010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3339964"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFB80_21020"
     CDS             complement(<1..841)
                     /locus_tag="VFB80_21020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN36330.1"
                     /translation="MSRGGDRQTIDRRSFVKASLIPATLPLTAVLARSTARAGEQAPA
                     GGTVPEIIDTNVHLFQWPFRRLKYDQTTLLVAKLRKHRIAQAWAGSFEAVLHKQLDLV
                     NRRLADECRTRGAGLLVPFGSVNPAAPDWQEDLRRCHEQYRMAGLRLYPTYHGYTLDH
                     SEFVSLLAAAQKRGLVVQIVMRLEDERVHHPAINVPLVNVAPLVDGLKKAPQAKVQLI
                     NSAGPLLGNNVADLVRQTQVTFDIAATEGNGGVGRLIEGKNYSYRGAIPAERLLFGSH
                     APYF"
     gene            complement(1275..2684)
                     /locus_tag="VFB80_21025"
     CDS             complement(1275..2684)
                     /locus_tag="VFB80_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36331.1"
                     /translation="MLTIPGPQARSCDGISRRSCLQIGTLALGGLALPEILRAEAAGG
                     GRQAAKGIIMVLLPGGPTHLDTFDLKPDAPAEVRGEFRPIATRVSGLEICELLPRLAA
                     IADKYAVIRSLVGFRDDHNTHWCSTGWESHPAMDASPIVAGFPPGDWPSLGAVLSRQL
                     GARVAGVPPCVDLTPTDPDARFILRTPPGQPGYLGPAHAAFEVNAVDRRNITLGGVDL
                     QRLADRQALLSGFDRFRRRVDRAGTDGLEEFHRQALAVLTSPRLAEALDLSREDRAVR
                     SRYGLDRSYPSEREGKTLLDQFLMARRVIEAGARCVTLAFCRWPFGRMLRGDYNWDWH
                     KDLFNEARGTLPLLDWGLSALLTDLDERGLLDEIAVVVWGEFGRTPKINANAGRDHWP
                     GVCSALVAGGGMQGGQVIGSTTKWAEQPLNRPIHFREVFATLYERMGIDVAKTQFHDL
                     AGRPQYLVGEHRPLPELVG"
     gene            complement(2882..>3702)
                     /locus_tag="VFB80_21030"
     CDS             complement(2882..>3702)
                     /locus_tag="VFB80_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN36332.1"
                     /translation="LRTVVDSLKRRAGEVRWGHGCKIGVYAQHVYTSLPEKQTVLDYL
                     ENAAAIGTKTQEILDLAGALLFRGDAVKKRIAVLSGGERARLCLAGLLLSEYNVLVLD
                     EPGNHLDVDTVEALADALLNYQGTVIFTSHDRHFMKRVATCIVEVRDGHVTNYRGDYE
                     SYLYSVNKEIEEGEREQAKGLSKAPPNPGKPGKTPPRALRRSEREIRKEMTNLERTIA
                     RLDDGKRQTNAQLMAATDPAEALRLHNEVSALTMQLAEAEDRWCQLQEEVDEAN"
BASE COUNT          615 a         1229 c         1207 g          651 t
ORIGIN      
        1 gaaaataggg cgcgtgggag ccgaacagca gccgctcggc gggaatcgcg ccgcggtagc
       61 tgtagttctt gccttcgatg agccgcccga cgccgccgtt gccttcggtg gcggcgatgt
      121 cgaaggtgac ctgagtctgg cggaccaggt cggcgacgtt gttgcccagc agcggcccgg
      181 ccgagttgat cagctgcact ttcgcctgcg gggctttctt cagcccgtca acgagcggcg
      241 caacattgac cagcggcaca ttgatcgcgg gatgatgaac gcgctcgtct tccagccgca
      301 tcacgatctg gaccacgagg ccgcgcttct gggccgcagc caatagcgac acgaactcgg
      361 aatgatcgag ggtgtagccg tgatacgttg ggtagagcct cagtccggcc atgcggtatt
      421 gctcgtggca gcggcggagg tcttcttgcc aatcgggtgc ggccggattg acgctgccga
      481 aagggacgag cagccccgcg ccgcgcgtgc ggcactcgtc ggccagccgg cgattgacga
      541 ggtcgagttg cttgtgcagc acggcctcga agctgcccgc ccaggcctgc gcgatgcgat
      601 gcttgcggag cttggcgacc agcagcgtcg tctggtcgta cttcagccgg cggaacggcc
      661 actggaacaa gtggacgtta gtgtcgatga tctccggcac agtgccgccc gccggcgctt
      721 gctcaccggc tcgggcagtg ctccgagcca gcaccgcggt cagcggcaac gtcgccggaa
      781 tcagcgacgc cttgacgaag ctccgccggt cgatcgtctg cctgtccccg ccgcgagaca
      841 tctaaaggcc cccgtcgttt tcaccggccc gccggtcgta cagagtacac ccagcagggc
      901 tcgaacctgc aaccttcggt tccgaaatcg ggtccaaatc gtcccaaacc tcaatgggtt
      961 atagccttcg ggcgacaggg atcaagcggg gtgcttttag cggtgctcgc ccaatttccc
     1021 cgccttgccg caggttgccg cttatggcac tgtggcggaa accaatggcc agtggagcga
     1081 cttggcgtct ccgcgcaggt cttggtgaat ccgccgtgag gccttcggcc caccttggcg
     1141 gcgatcacat cgcgcagcgg ctcgcacttg ccggctgggc ccggagatcc cactctcaga
     1201 agtggctgtg agcagcgcct cggcggactg ctgaagcatt actatcgccc acatccactc
     1261 tcccaacgat ccaatcaacc aaccaactcc ggtaatggcc gatgttcgcc gaccagatac
     1321 tgtggccggc cggccaggtc gtggaattgc gtcttggcaa cgtcgattcc catgcgctcg
     1381 taaagcgtgg cgaagacctc gcggaaatgg atagggcggt tcagaggttg ttcggcccac
     1441 ttggtcgtgg aaccgatgac ttgcccgccc tgcatgccgc cgccggccac cagggcgctg
     1501 cacacgcccg gccagtggtc tcggccggcg ttagcgttga tcttcggcgt gcggccgaac
     1561 tcgccccaaa cgacgacggc aatctcgtcg agcagaccgc gctcgtcgag gtcggtcagc
     1621 agcgcggaga gcccccaatc caagagcggc aacgtgccgc gcgcttcgtt gaacaggtct
     1681 ttgtgccagt cccagttgta gtcgccgcgg agcattcgcc cgaagggcca gcggcagaag
     1741 gcgagcgtga cgcaacgggc acccgcttcg atcacgcgcc gcgccattag gaactggtcg
     1801 agcaacgtct tgccttcgcg ctcgctggga tagctgcggt ccaggccata gcggctgcga
     1861 accgcccgat cctcgcggct caagtccagg gcctcggcca gccgcggcga cgtgaggacg
     1921 gcgagcgcct ggcggtgaaa ctcttcgagt ccgtcggtcc ctgcccgatc gacccggcgg
     1981 cggaaccgat cgaagccgct caggagggcc tgacggtccg ccaggcgctg caaatcgacg
     2041 cctcctagcg tgatgttgcg gcgatcgaca gcattgactt caaacgcggc gtgagccggg
     2101 ccgaggtagc cgggctgccc cggcggcgta cggaggatga accgggcgtc gggatcggtg
     2161 ggcgtcagat cgacgcacgg cggcacgccg gcgacccgcg ctcccaactg ccgcgacagc
     2221 accgcgccga gcgaaggcca gtcccccggt ggaaaccccg ccacgatggg cgacgcgtcc
     2281 atcgccggat gcgattccca gccggttgaa caccagtgcg tgttgtgatc gtcgcgaaat
     2341 ccgaccagcg agcggatgac ggcatatttg tctgcgatcg cggccaaccg cggcagcagc
     2401 tcgcagattt ccagaccgga caccctggtg gcaattgggc ggaactcgcc acgaacctcg
     2461 gccggcgcat cgggcttcag atcaaacgtg tccaggtgcg tggggccgcc gggcagcagc
     2521 accatgatga tgccctttgc cgcctgccgc ccgccgccgg cagcttctgc ccgcaggatc
     2581 tccggcagcg ccaagccgcc caatgccaga gtgccaattt gcaggcaact gcgccgcgag
     2641 atgccgtcac acgaccgcgc ctgcggcccc ggaatggtca gcatggcccc agcctccagt
     2701 gaccgaataa cgcatcaacc ctcgccaatc tatccagtcg gcgtgcgagc cgcaatgcgt
     2761 ttccattgag ccgatacctt gaccacgaac agcaggttgg tcgaccctgt caggctcagc
     2821 cgcggcgggg gctggatcgc gagagcccac ggccggtctt ctctgccgct cacgtgccag
     2881 ctcaattggc ctcatcgact tcttcctgaa gctggcacca ccgatcttcg gcctcggcga
     2941 gttgcatcgt cagcgcggag acctcgttgt ggaggcggag agcctcggcc ggatcggtcg
     3001 cggccatgag ctgagcgttg gtttgacgct tgccatcgtc caggcgggcg atcgtccgtt
     3061 caagattggt catctccttg cgaatctcgc gctcgcttcg ccgcagagcg cggggtggcg
     3121 tcttcccggg tttgccgggg ttgggcggcg ccttgctcag gcccttggcc tgctcccgct
     3181 cgccttcctc gatttccttg ttgaccgaat agagatacga ctcgtagtcg ccgcgatagt
     3241 tcgtcacgtg gccgtcgcgg acttccacga tgcaggtcgc cacccgcttc atgaagtggc
     3301 ggtcgtggct ggtgaagatc acggtgccct gatagttgag cagcgcgtcg gccagcgcct
     3361 cgacggtatc gacatccagg tggttgcccg gctcgtccag taccagcacg ttgtattcgc
     3421 tcagcaagag tcccgcgagg cagagccggg cgcgttcgcc gccggagagg acggcgatcc
     3481 gctttttcac ggcgtcgccg cggaacagca gcgccccggc caggtcgaga atctcttgcg
     3541 tcttcgtgcc gatggccgcg gcgttctcga ggtagtccag cacggtctgc ttctcgggca
     3601 ggctggtgta gacgtgctgg gcgtagacgc cgatcttgca gccgtgtccc cagcggactt
     3661 cgccggcgcg ccgcttgaga gagtccacca ccgtgcgcaa ga
//
LOCUS       DASRJB010000801         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3901786, whole genome shotgun sequence.
ACCESSION   DASRJB010000801 DASRJB010000000
VERSION     DASRJB010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3901786"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFB80_21035"
     CDS             <1..645
                     /locus_tag="VFB80_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36333.1"
                     /translation="ETWRLRRRYGEKYYRQFWSQLIYHLGMSHALGSDKRFVARIDAQ
                     EYRAEDKVLLTVEAYDENYEPLGAETLADGAMTAELVKPNGDVTPLSVPMLRRGVFEL
                     RVPVFAAGQYSLRVKDPITGKSEEQPFEVLPLSAERRRGVRDEKLQNDLAQRTGGKAY
                     DLTTVDQLVSDLDVKPLLERQTRNHGLWTTPLWFGLVVALMLGEWLGRKMIRLS"
     gene            642..2714
                     /locus_tag="VFB80_21040"
     CDS             642..2714
                     /locus_tag="VFB80_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36334.1"
                     /translation="MTRLSTLRAQLASLRRARLTVRQAAAWSAVAIAALWSLAILFAL
                     DVRFEFGRVERAILLVAAIGLVVWAARRFALPYLGVRETEEDMALLVERRQQIDSDLI
                     AALQFESPQAGTWGSPQLEGAVIDYVAQVGRGINVFDGFSTTDLWRRGGLLAGTLLVA
                     LIATALFPGHVWAFAQRLLLGRTHYPTDTLIEQIVVNQREVLNRASHGGQPLDARCAQ
                     GRPIQFLVECRGALPEKGSARLTATGGSRSRTELTVSRLSLDDRQARLTQAAQKMKAA
                     AADDSIDISQPWRDEVISLVKFDAPQTAAALALLKERSELSAALERLNAALAIWPADA
                     STRGIYVGVLDRLVDGVEYKLTLGDAWTDPARVTMIPLPIVQRSLTPTPPMYAKTTEN
                     SQSVTTPQMAVLEGTRIDVAVECVNDKPLASAWMNVRQGNETQRLELVKADETGLRWR
                     LDDPVAGKLRVPSKGNGTRSVPTTYSPLARVTKELSYEIQVLDADGLSLESPLRGTIH
                     IKPDRAPTGGAELVHKVVLPEAQPVIEYRASDDFGVARVRLAVAIERQQGDIVPTMPP
                     GESASHTGPMVESTGSQMESATLDLYAADAAGKKLEPKLTGKYALALGKLPLAQKLVK
                     GDRVKLTLEVTDFRGDAGGASYFSDPLVLEISDESGVLAAISEADERSEQRLTDIIKR
                     QLGIGESP"
     gene            2711..>3600
                     /locus_tag="VFB80_21045"
     CDS             2711..>3600
                     /locus_tag="VFB80_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36335.1"
                     /translation="MKWMQGLLVAALAWMFLWTMLAPALAQQDEGSVFRRKQQAQEKA
                     RALAGELVNAVLDIQLRQLAENGLAERAIYKDIASMKGNIGELMKDDMEAIVQLLVQA
                     QEGKQEQRLAKFNEARGKIREVVVQLMAERQKLYRRMQIAKLAAQVRELIAIQTKTRT
                     VTRSLPERKLEERERLTLTTIEDQADVQKLFYQLVATLEDVATWGGQVGAGASDGLRI
                     VKAAQVDQELKRAGATLAKADIPQATDAQTKVIAGLKRLLEKLEETQGLIESDREAAI
                     RMVREMMKKQEQVREQTKVA"
BASE COUNT          679 a         1104 c         1268 g          549 t
ORIGIN      
        1 gagacgtggc ggctgcggcg gcggtatggc gagaagtact accgccagtt ctggtcgcag
       61 ctcatttatc atctcggcat gagccacgcc ctggggagcg acaagcggtt cgtggcgcgg
      121 atcgacgcgc aagaataccg ggctgaagac aaggtgctgc tgacggtcga ggcgtacgac
      181 gagaactacg agccgctggg ggccgagacg ctggccgacg gcgcgatgac cgcggagctc
      241 gtgaaaccca acggcgacgt gactccactg tcggtgccga tgctccgccg cggcgtgttc
      301 gagctgcgcg tgccggtgtt cgccgccggg caatatagcc tgcgggtcaa ggacccgatc
      361 acgggcaaga gcgaggagca gccgtttgag gtattgcctc tgtcggccga gcggcgccgc
      421 ggcgtgcgcg atgagaaact ccagaacgac ctggcccagc ggaccggcgg caaagcgtac
      481 gacctgacga ccgtggacca gctcgtcagc gatctcgacg tgaaaccgct cctggagcgg
      541 cagacgcgca atcacggcct gtggacgacg ccgctctggt tcggtctggt ggtggcactg
      601 atgctcggcg aatggctcgg ccgcaagatg atccggcttt catgacgcga ctatcgacac
      661 ttcgagcgca attggccagc ctgcggcggg cgcggctgac cgtgcgccaa gcggccgcct
      721 ggtcggcggt ggcgatcgcc gcgctttggt cgctggcgat cttgtttgcg ctcgacgtgc
      781 gctttgagtt tgggagggtc gagcgggcga ttctgctggt ggccgcgatc ggcctggttg
      841 tgtgggccgc gcggcggttc gcgctaccgt atctcggtgt tcgcgagacc gaagaggaca
      901 tggccctgct cgtcgagcgg cggcagcaga tcgacagcga cctgatcgcc gccctccagt
      961 tcgaatcgcc gcaggcgggc acgtggggct cgccgcagct cgaaggggcg gtcatcgact
     1021 acgtcgctca ggtcggccgc gggatcaacg tcttcgacgg cttttccacc accgacctgt
     1081 ggcgtcgcgg cggcctgttg gcgggtacgc tgctggtcgc gctgatcgcg acggcgttgt
     1141 tccccggcca tgtgtgggcc ttcgcccagc ggctgctgct tggccgcacg cattacccca
     1201 ccgacaccct catcgagcag atcgtcgtca accagaggga agtcctcaac cgcgccagcc
     1261 acggcggcca gccgctcgac gcccgctgtg cgcaggggcg gccgatccag tttctcgtcg
     1321 aatgccgcgg cgcgctgccg gaaaaggggt cggcgcggct aactgcgacc ggcggttcac
     1381 ggtcccgcac cgagctgacc gtgtcgcgac tgtcgctcga cgaccgccag gcgcggctga
     1441 cgcaggcggc gcagaagatg aaagccgccg cggcggacga ctcgatcgac atctcgcagc
     1501 cgtggcggga cgaagtgata tcgctggtca agttcgatgc gccacagacg gccgccgcac
     1561 tggcgttgct caaggagcgg agcgaattgt ccgcagcttt ggaaaggctc aacgcggcgt
     1621 tggcaatctg gccggccgat gccagcactc gcggcattta cgttggcgtg ctcgatcggc
     1681 tggtggacgg cgtcgagtac aagctgacgc tcggcgacgc ctggaccgat ccggcccgcg
     1741 tgacgatgat cccgctcccg atcgtgcagc gctcgctcac gccgacaccg ccgatgtacg
     1801 cgaagacaac cgagaactcg cagagcgtca ccacgccgca gatggcggtg ctcgaaggga
     1861 cgcgaatcga cgtggccgtc gagtgcgtca acgacaagcc gctggccagc gcgtggatga
     1921 acgttcgaca ggggaacgag acgcaacggc tggagctggt gaaagcggac gagacaggat
     1981 tgcggtggcg gctcgatgac cccgtagcag gcaaactccg tgtgccgtcc aagggaaacg
     2041 gcacacggag tgtgcctact acttattcac ctctcgcccg cgtcaccaag gaactgagct
     2101 acgagatcca agtcctcgat gccgacggcc tgagcctcga atcgccgctg cgggggacga
     2161 ttcacatcaa gcccgatcgc gcgccgacgg gcggagccga actggttcac aaggtcgtgc
     2221 tccccgaggc ccagccggtg atcgaatacc gggccagcga cgatttcggc gtggcccgcg
     2281 tgcggctggc ggtcgccatc gagcggcagc agggggacat cgtgccgacg atgccgccgg
     2341 gtgaaagcgc gtcgcacacg ggcccgatgg tggaatcgac cggcagccag atggagtcgg
     2401 caacgctcga cctctatgcg gcggatgcgg ccggaaaaaa actggagccc aagctcacgg
     2461 gcaagtacgc actggcgctg ggtaagctgc ctctggccca gaagctggtc aaaggcgacc
     2521 gcgtgaagct gacgctggag gtgaccgatt tccgcggcga cgcgggggga gcgagctact
     2581 tcagcgatcc cttagtgctg gaaatttcgg acgaaagcgg cgtgctggcg gccatttctg
     2641 aggccgacga gcgctccgag cagcggctga ccgacatcat caaacgtcaa cttgggattg
     2701 gagaatcgcc atgaaatgga tgcaaggact cttggtggcg gccctggcgt ggatgtttct
     2761 gtggacgatg ctcgcgccgg cgcttgcgca gcaggacgag ggcagcgtct tccgccgcaa
     2821 gcagcaggcc caggaaaagg cccgggcgct ggccggcgag ctggtcaatg ccgtgctcga
     2881 catccagctt cgccagcttg ccgagaacgg actggcggag cgggcgattt acaaggacat
     2941 cgcctcgatg aagggaaata tcggcgagtt gatgaaggac gacatggagg cgatcgtgca
     3001 gcttctcgtg caggctcagg aagggaagca ggagcagcgg ctggcgaagt tcaacgaggc
     3061 ccgcggtaaa attcgcgagg tcgtcgtcca gctcatggcc gagcggcaga agctctaccg
     3121 ccggatgcag atcgccaaac tcgccgcgca ggttcgcgag ctgatcgcca tacagaccaa
     3181 gacccgcacg gtgacgcgat cgctgcccga gcgcaagctc gaggagcgcg agcgactgac
     3241 gttgacgacg atcgaagacc aggccgacgt gcagaaactc ttctaccagc ttgtggcgac
     3301 gctcgaagac gttgccacct gggggggcca ggtcggtgcc ggcgcttcgg acggcctgcg
     3361 gatcgtcaaa gcagcccagg tcgatcagga actaaagcgg gccggcgcga cgctggccaa
     3421 ggccgacatt ccgcaggcga ccgacgccca gaccaaagtc atcgccggac tcaagcggct
     3481 gctggaaaag ctggaagaga cgcaggggct gatcgagagc gaccgcgagg cggcgatccg
     3541 catggtccgc gagatgatga agaagcagga gcaggtccga gagcagacga aagtcgccga
//
LOCUS       DASRJB010000802         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1088102, whole genome shotgun sequence.
ACCESSION   DASRJB010000802 DASRJB010000000
VERSION     DASRJB010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088102"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..>3400)
                     /locus_tag="VFB80_21050"
     CDS             complement(225..>3400)
                     /locus_tag="VFB80_21050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN36336.1"
                     /translation="SPHERAAFLGEACQSDAALRAEVEGLLAALDRAGEFLRQPAAAV
                     ETVEPTCGADKPGTIVADRYKLLEQIGEGGMGTVWVAEQIEPIKRRVALKLIKPGMDS
                     RQVLSRFEAERQALALMDHPHIARVFDGGLTAEGRPFFVMEYVKGVPITDYCDQARLP
                     IAERLALLISVCQAVQHAHQKGIIHRDLKPSNILVCLYDGQPVPKVIDFGLAKAMHQP
                     LTERTLYTAHGLMVGTPLYMSPEQAELNNLDVDTRTDIYSLGVLLYELLTGSTPLEQR
                     QFKDAAFAEILRLIKEEEPARPSLKLSGSASLPSIAAQRSVEPAQLSRTVKGDLDWIV
                     MKSLEKERSRRYETANGLARDIERYLHDEPVEACPPSLRYRLGKVLRRHRGPAIAAAL
                     VLLALLVGMVGTTLGLVQANAERNKARKLAALAAKEATNAEQAAAAEAEQRRRADEEA
                     AAARAVRDFLQDDLLRQASMIHQAETGSVIDDGTRFVLNPTINELLDRAAAELTADKI
                     EAKFPKLPFVQAEALKAVGDAYAGVAPEKALDHLQRAIELYRQSRGPDDPATLSARHS
                     LGIALGACRKDREAEQVLSAVLRDRARVLGPLHVDTFATRDFLGRVHLWLKSPDAVTY
                     FEKLRDDAGSKFGQHHIQTLKARFHLALAMQYAGQLEAGLREMESLKALGDRLNLRED
                     HPYALSAAMETGLAYKAAGRHTEAIEAFEKCLALVERWKLGPQPIWAVRHQLGWSYLS
                     AGRRDDAIRTFELNLAIAGRAGANDVTTMEALGWAVREVDQQRCIALYTQARDLRFER
                     SGANDTEALRVAVKLSTLLREFKHNEQALKSLDEFLPRLTTVHGADHLAVLKAQIEKA
                     RCLAALTQWEAANLLFRETIPKMEKSRGSGDMETLEARYWWMLAARDCGQLPRAIELA
                     DDLAPRWRAARTRPTRVADVRIHQGSNLVRLQRPAEGEPHLREALAIYLKETPQAWNT
                     ANCKQWLGTALARQEKFAEAEELLVAAFNDMTERIAQTPVWGQNHRAEIAQRLAAIYS
                     AQDKTAESAHWQSVWEQLAGAAPPSGK"
BASE COUNT          526 a         1153 c         1083 g          638 t
ORIGIN      
        1 ggtctgtcgg cgatcccaga gcagaccgcc gatggccagt tgctgggcga cgatgacgac
       61 gagcagtagc gcggtggaca gcgaaaatcg gattcgcggc acggtatcgc ctcctggaat
      121 tgcactgaag gtgccgccaa cttaaccgga gcgtctagca atggcaaatt gcgttgatcg
      181 ggcgctggcc ggaaaaccgc tgccggctcg gccggcgctg gcgttcattt tccggacgga
      241 ggcgctgctc cggccagctg ctcccatacg ctttgccagt gggcagactc ggcggtcttg
      301 tcttgcgccg agtagatcgc ggccagacgc tgggcgatct cggcgcgatg gttctggccc
      361 cacaccggcg tttgcgcgat ccgctcggtc atgtcattga aggccgccac cagcagttcc
      421 tcggcctccg cgaatttctc ctgcctggcc agcgccgtgc cgagccactg cttgcagttg
      481 gctgtgttcc aggcctgcgg ggtctccttc aagtaaatgg cgagggcttc ccgtaggtgc
      541 ggctccccct cggccggccg ctgcaggcgg acgaggttgc ttccctgatg gatccgcacg
      601 tcggccacgc gcgtcggcct ggtccgcgcc gcccgccagc gcggggccag gtcgtccgcc
      661 aactcgattg cccgcggcaa ttggccgcaa tcccgtgcgg cgagcatcca ccaatagcgg
      721 gcttccagcg tttccatatc gccgctgcca cgcgactttt ccattttggg aatcgtctct
      781 ctgaagagca ggttcgccgc ttcccattgc gttagcgccg ccagacagcg ggccttttcg
      841 atttgggcct tgagcacggc aagatgatcc gcgccatgga cggtggtgag tcgcggcagg
      901 aactcgtcga gcgatttcag agcctgctcg ttgtgcttga actcgcgcag cagtgtagac
      961 aacttgacgg ccacgcgtag cgcctccgtg tcgttagcgc cgctgcgctc gaaacgcagg
     1021 tcgcgggcct gcgtgtacag cgcgatgcag cgctgctggt ccacttcgcg cacggcccag
     1081 cccaaggcct ccatggtcgt cacgtcgttg gcgccggcgc gtccggcgat ggccaagttc
     1141 agctcaaaag tgcggatggc gtcgtcgcgg cgaccggccg acaggtaact ccaaccaagc
     1201 tgatgccgca cagcccagat gggttgcggg ccgagcttcc agcgctccac cagcgccagg
     1261 cacttttcga aggcctcgat ggcctcggta tggcgaccgg cggctttgta ggcgagcccg
     1321 gtttccatgg ctgccgatag cgcataggga tgatcctcgc gcaggttcaa gcggtcgccc
     1381 agagctttca aggattccat ctctcgtagc cccgcctcga gctggccggc gtattgcata
     1441 gccaacgcca ggtgaaatcg ggccttgagc gtttgaatgt gatgctggcc gaatttactg
     1501 ccggcgtcgt cgcgaagctt ttcgaaatag gtcacggcgt ccgggctttt cagccacaaa
     1561 tgcacgcggc ccagaaaatc gcgcgtggcg aacgtatcga catgcagcgg ccccagaacg
     1621 cgggcccgat ctctcagcac cgccgagaga acttgctctg cctcgcggtc ctttcggcaa
     1681 gcgccgaggg ctatgcctag cgaatgccgc gcggagagtg ttgccggatc gtccgggccg
     1741 cgcgactgcc ggtacagttc gatcgcccgc tgcaggtggt cgagggcctt ctcgggcgcg
     1801 actccagcgt aggcatcgcc caccgccttg agcgcttcgg cctgcacgaa aggcagcttc
     1861 gggaacttcg cctcgatctt gtccgccgtc agctcggccg cggcccgatc gagcaactcg
     1921 ttgatcgtcg gattcagcac aaatcgagtg ccgtcgtcga tcacggagcc ggtctccgcc
     1981 tggtgaatca tcgacgcctg ccgcagcagg tcgtcctgca gaaaatcgcg caccgcacgg
     2041 gccgcggccg cttcttcgtc ggcccgccgc cgctgctccg cttcggcggc agccgcctgt
     2101 tctgcatttg tggcctcttt cgccgccagt gccgcaagct tgcgggcctt gttccgctcg
     2161 gcatttgcct ggaccaggcc cagcgtcgtg ccgaccatcc caaccagcag ggccagcagc
     2221 accagcgccg ccgcgatcgc cggcccgcga tgccgccgca gcactttgcc cagccggtac
     2281 cgcagcgacg gcgggcaggc ctcgaccggc tcatcatgca ggtaacgctc gatgtcgcgg
     2341 gccagcccat tggcagtttc gtaccgccgg ctgcgttcct tctccagcga cttcatcacg
     2401 atccaatcca ggtcgccctt cacggtccgg ctcagctggg ccggctccac gctccgctgg
     2461 gccgcgatgc tcggcaggct ggccgagcca ctcagtttga gactcggccg cgccggttct
     2521 tcttccttga tcagcctgag gatttccgca aaggccgcgt ccttgaactg ccgctgctcc
     2581 agcggcgtgc tgcccgtcag caattcgtag aggagcacgc ccagcgaata gatgtcggtc
     2641 cgcgtatcga cgtcgaggtt gtttaactcg gcctgctcgg ggctcatgta cagcggcgtg
     2701 ccgaccatca ggccgtgggc cgtgtagagc gtccgctcgg tcagcggctg atgcatcgcc
     2761 ttggccaggc cgaagtcgat caccttgggg accggctggc cgtcatacag acaaaccagg
     2821 atgttcgagg gcttcaggtc gcggtggata atgcctttct ggtgggcgtg ctgcactgcc
     2881 tggcagacgc tgatcaagag cgcgagccgc tcggcgatcg gcaagcgggc ctggtcgcaa
     2941 tagtcggtga tcggcacgcc cttgacgtat tccatcacga agaacggccg gccttcggct
     3001 gtcaggccgc cgtcgaacac gcgggcgatg tgtggatggt ccatcaaggc cagggcctgc
     3061 cgttcggcct cgaaccgcga taacacctgc cggctgtcca tgcccggctt gatgagcttg
     3121 agggccaccc ggcgcttgat cggctcgatc tgctcggcga cccagaccgt ccccatgcct
     3181 ccttcgccga tctgctccag cagcttgtag cggtcggcta cgatcgtgcc gggcttgtct
     3241 gcaccgcatg tcggctcgac cgtctccacc gcagccgccg gctgccgcag gaactcgccg
     3301 gctcggtcga gggcagccag caggccctcg acttcggccc gcaaggccgc atccgactgg
     3361 caagcctccc ccaggaaggc cgctcgctcg tggggcgaca
//
LOCUS       DASRJB010000803         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_237509, whole genome shotgun sequence.
ACCESSION   DASRJB010000803 DASRJB010000000
VERSION     DASRJB010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237509"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFB80_21055"
     CDS             <1..567
                     /locus_tag="VFB80_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36337.1"
                     /translation="LRLARHRAIVLGHRIAIVGMSLWLIAGIAFPIGIHAYAQKFMPE
                     VYAKFILSMFACGIISCCLPFLATTWLTIRVYFPLLLANSTPEPNEQKKLMNLSWYAG
                     IYFAVSPVAPLIAVLLILLSSPRIPTPGDPDPRLAIGGLIISSILGFGAAYLVWQRIR
                     GDLAALAVVTRPPDQIGTTSQSTDSFLA"
     gene            complement(615..1364)
                     /locus_tag="VFB80_21060"
     CDS             complement(615..1364)
                     /locus_tag="VFB80_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36338.1"
                     /translation="MCCFTGVVDSVSSTKIFARPSSEGRQFVVYHMLYKAKEELAMVL
                     PIPTPAKSADDAVKFINLEEYDSFFAALENGFPRPRPKSDAAPAGAIPPLPKAPLPVV
                     EVGSFEASFVPSTSDFSRLDKRFRLSDGVWEKLPTYKDFGFAVFKLKAGQPKIHPIAF
                     EFPRARADRIFFPTVHIHDGEVHAEAEFDHVLYLQPGSAQSRTPRDWEESPQPAGMFM
                     DVKKSKGLLAGDLHVYRRRMRGKQKNADTLV"
     gene            complement(1859..>3358)
                     /gene="ggt"
                     /locus_tag="VFB80_21065"
     CDS             complement(1859..>3358)
                     /gene="ggt"
                     /locus_tag="VFB80_21065"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN36339.1"
                     /translation="EPTAEKVGQTVLASGGNAIDAAVAAALVAAVAAPHQTGIGGYGG
                     HMTLFAAKASKLTCIDFNSHAPAAARPDMFPLDAAGKVVGQKNMYGWLAVGVPGILAG
                     LHLALTKYGTKPFREIVQPAIGLAREGFPIGAAAAAIKSSANQLAADPGSRALYFQDG
                     QPLAASDRFRNPELAKLLESLAAANSVESFYRGEIAQRIAAAFAKNGGLVTAEDFAAY
                     RAHEVEPLKITWGEWTIRTAPLTAGGATALHALLLLQELHWAARDAAAAETLQLQVEA
                     LRYAWQDRLELFGDPQGGASPLERVLQPAAIRTAAERIAKAVAARQPLPVRTTSQPDQ
                     GTIHLSAADREGNLAAVTLTHGGSFGARVTVPGLGLTLGHGMSRFDPHAGHPNAPGPH
                     KRPLNNMCPTIVARGGRPLIALGARGGRKIPNAVAEVLLQLVAREKPLADAVAAPRLH
                     TEGQLALNFEKSWPSAVLEELKARGYQVTVAASATVSAAEAGSLASAMR"
BASE COUNT          568 a         1093 c         1067 g          630 t
ORIGIN      
        1 ctgcgcttgg ctcgccatcg ggccatcgtg ctcggtcacc gcatcgccat cgtgggaatg
       61 tcgctgtggc tgattgccgg catcgccttc cccatcggca tccacgcgta cgctcagaaa
      121 ttcatgcccg aagtgtatgc caagttcatt ctgtccatgt tcgcctgcgg gatcatttcc
      181 tgttgcctgc cgtttctggc gacgacctgg ctgacgatcc gcgtctattt tccgctgctg
      241 ttggccaaca gcacgcccga gcccaacgag cagaagaagc taatgaacct gagttggtac
      301 gcgggcattt atttcgccgt ttcgccggtc gcgcccttga ttgcggtgct gctcatcctg
      361 ctcagcagcc cgcgcattcc aactcccggc gatcccgatc ctcgcctggc gatcggcggg
      421 ctgatcatct ccagcatcct ggggttcgga gccgcctact tggtctggca gcggattcgc
      481 ggcgacctgg cggcactggc cgtggtcact cggcctccag atcagatcgg cacgacgagc
      541 caatcgactg attcgttcct ggcgtaaaaa aaaacgcacg gtgggacaag cgagcggaga
      601 aagccccgta cggcttacac cagcgtgtcg gcgttctttt gcttcccgcg cattcgcctg
      661 cgatagacgt gcaggtcgcc ggccaaaagg cctttggact ttttcacgtc catgaacatg
      721 ccggcgggct gcggcgattc ttcccagtcg cggggcgtgc gcgactgggc cgagccgggc
      781 tgcaggtaga gcacgtggtc gaactcggct tcggcgtgga cctcgccgtc gtggatgtgg
      841 accgtgggaa agaagatccg gtcggcccgc gcccgcggaa attcgaacgc gatcgggtgg
      901 atcttcggct gcccggcctt gagcttaaag accgcgaagc cgaaatcctt gtacgtcggc
      961 agcttttccc acacgccgtc gctcaagcgg aagcgcttgt cgagccgcga gaagtcgctg
     1021 gtgcttggca cgaacgacgc ctcgaagctg ccgacctcga cgaccggcaa cggcgccttg
     1081 ggcaagggag gaatcgcccc tgcgggcgcg gcatcgctct tgggccgcgg tctgggaaaa
     1141 ccgttctcca aagcggcaaa gaagctgtcg tactcttcca ggttgatgaa tttcaccgcg
     1201 tcgtcggccg atttcgcggg cgtcggaatc ggcagcacca tcgccagttc ttccttcgcc
     1261 ttgtagagca tgtgatagac gacgaactgc cgcccctcgc tactggggcg ggcgaaaatc
     1321 ttcgtggacg acaccgaatc gacgaccccg gtgaaacagc acatagccgg ctcctttcaa
     1381 ttgagacgaa actcgggggc agttcgattg ctgcccaagc ccagcgactc aaaatgagac
     1441 accccccagg cggctgcagc aatcgatccg tcgtctggag gcggaccgcg cggcccgtcg
     1501 gttgccctta agggccggcc gcgaatataa cgaaaaccgc cctaggcaca gcttcgcgcc
     1561 ctcgcccgct gtgtgccact cgcgccgcaa ctgtatgaca ggtgaggact tccggcattc
     1621 cgccggcgtt ttctggcctt agcgtttgaa gtgttagtga gcctggcggg gagttttcgt
     1681 tatatcttgc tgcccagcgg agcggcggag gttccgcctc agttcacaac gaccaatctg
     1741 agaatgagcg agcgaccttg gcggcgggcg gccaaaacgg cagtccgccg cgccaaaggg
     1801 aactatactg caaatagcgt actaatgttc acggttgtag caggccgttg gcgattctct
     1861 agcgcatcgc cgacgccagc gagcccgcct ctgcggcact gaccgtcgcg ctggcggcga
     1921 ctgtgacctg gtagccgcgc gccttcaatt cttccagcac cgcactcggc caggactttt
     1981 cgaagttcaa ggctaactgt ccttcggtgt gcagccgggg ggcggcgacg gcgtccgcga
     2041 ggggcttttc gcgggcgacg agctgcagca ggacttcagc gacggcattg ggaatcttgc
     2101 ggccgccgcg agcacctagc gcaatgagcg ggcggccgcc tctggctacg atcgtggggc
     2161 acatgttgtt gagcgggcgc ttgtgcggtc ccggcgcgtt gggatggccc gcgtgcggat
     2221 cgaaccggga catgccgtga ccgagcgtca gacccagtcc cggcacggtg acccgcgcgc
     2281 cgaaagagcc gccatgcgtg agcgtcaccg cggcgagatt tccctcgcga tctgcggcgc
     2341 tcaggtggat tgtgccctga tctggctgcg aggtcgtccg cacgggtaag ggctgtctgg
     2401 cggcgacggc tttcgcaatc ctttcagcag ccgtgcgaat cgcggccggc tgaagcaccc
     2461 tctccagcgg cgacgcccca ccctgcggat ctccgaagag ttcgaggcgg tcctgccagg
     2521 cgtagcgcaa cgcctccacc tgcagctgga gcgtttcggc agctgctgcg tcgcgagccg
     2581 cccaatgcag ctcttgcagc agcagcagcg cgtgcagcgc cgtcgcaccg ccggcggtca
     2641 gcggcgcagt gcgaatcgtc cactcgcccc acgtgatttt cagcggctcc acttcgtgcg
     2701 cccgatacgc cgcgaagtcc tccgccgtca ccaggccgcc attcttggcg aaggcggcgg
     2761 cgatccgctg ggcaatctcg ccgcgataga acgactcgac ggaattggcc gctgccagcg
     2821 attccagcag ttttgccagc tcgggatttc taaagcggtc gctcgccgcc agcggctggc
     2881 cgtcttggaa atagagggcc cgcgaacccg gatcggcagc caactgattg gccgaggact
     2941 tgatcgccgc tgcggcggcg ccaattggaa agccttcgcg ggccagtcca atcgccggct
     3001 gcacgatctc gcgaaatggt ttcgtcccgt atttcgtcag ggccagatgc aagccggcga
     3061 gaattcccgg cacacccacc gccagccagc cgtacatgtt cttttgaccg acgaccttac
     3121 cggcggcgtc gagcggaaac atgtcgggcc gcgcagccgc gggagcgtgg ctgttgaagt
     3181 cgatgcaggt gagcttgctg gctttcgcgg cgaagagagt catgtgcccg ccgtaccctc
     3241 cgatcccggt ctgatggggc gcggcaacgg ccgccaccag cgccgccgcg acggccgcgt
     3301 cgatcgcgtt gccgccggag gccagcaccg tttgaccgac tttctccgcc gtcggctc
//
LOCUS       DASRJB010000804         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3341510, whole genome shotgun sequence.
ACCESSION   DASRJB010000804 DASRJB010000000
VERSION     DASRJB010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341510"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFB80_21070"
     CDS             complement(<1..476)
                     /locus_tag="VFB80_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36340.1"
                     /translation="MPRNKISIAAALLLLTFPWAALAQDKLPTVAVVTTEWRHNSHAD
                     VIASRLVQGYNLDGKGEFPKLKLVSAYIDQFPANDMSRRLAMEHGFTIYDSVEKALTL
                     GGEKLAVDGVLLIAEHGKYPESETGSIQFPKRRMFGEIVKVFEKSGRVVPVFSDKH"
     gene            complement(495..761)
                     /locus_tag="VFB80_21075"
     CDS             complement(495..761)
                     /locus_tag="VFB80_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36341.1"
                     /translation="MTRYTVVWLESAIDDLARFWLKTVHKREVTESADLADEYLATDA
                     HHKGELLAEGLRAVTVGLLRQYFTVREFDRVVEVAYVRRVVRGP"
     gene            complement(758..976)
                     /locus_tag="VFB80_21080"
     CDS             complement(758..976)
                     /locus_tag="VFB80_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36342.1"
                     /translation="MRDIVVSDDFLKTLAESGGDIRFLNARGEVLGRFEPELTAEELA
                     EIRRRLASNEPRYTTKQMLERLNEKAAQ"
     gene            complement(1066..2202)
                     /locus_tag="VFB80_21085"
     CDS             complement(1066..2202)
                     /locus_tag="VFB80_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36343.1"
                     /translation="MARPFNIGMIGYGFMGRAHSNAYRKVGNFFDLEYQPVLKAACAR
                     DAAKVKPFADKWGYESIETDWRKLLARKDIDAVDICTPNNLHKEIAIAAAEAGKAILC
                     EKPLAMNAAEGEVMCRAVEKAKVPNIVWYNYRRIPAVTFAKQIIDSGKLGKIFHYRAN
                     FLQDWTISADLPQGGAGLWRLDVAAAGSGVTGDLLAHCIDTAIWLNGNVANVTAMTET
                     FIKERKHNLTGKVEKVGIDDACAFLCRFTNGSLGLFESTRYARGHKALYTFGINGEKA
                     SIKWDLHDLHRLQWFDHRTEGPLRGWSSIHVTDGEHPYMGKWWVPGLAIGYEHSFVHQ
                     VADFLDGLAKNQPAGPTFRDALETQKICDAVLASAKSGAWTNVG"
     gene            complement(2298..3338)
                     /gene="bioB"
                     /locus_tag="VFB80_21090"
     CDS             complement(2298..3338)
                     /gene="bioB"
                     /locus_tag="VFB80_21090"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZN36344.1"
                     /translation="MIHTAVEPLSETSTTIAWSDLAERVLAGHQANNDEALAILRAPD
                     EELLDLLAAAYRIRRRWFGNTVQLYFLMNAKSGLCPEDCHYCSQAKTATSDIPRYNLL
                     SREKLLDGARLAAERGSKTYCIVISARGPSEREIQAVEKIVPEIKARHDLEVCACLGL
                     LTPEQARRLAACGVNKVNHNLNTSAEFYGQICTTHTYQDRVETLNAVRAAGLELCSGG
                     IIGMGERDSDVVQMALALRELAVESIPLNFLIDIDGTPLEHSRRLNPRYCLKALAMFR
                     FVNPRSELRIAGGRELHLGSLQALGLYAANSMFVGDYLTTKGQPPEADYRMIDELGFV
                     VTRHEEVPAGHS"
     gene            3425..3994
                     /locus_tag="VFB80_21095"
     CDS             3425..3994
                     /locus_tag="VFB80_21095"
                     /inference="COORDINATES: protein motif:HMM:NF016361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HZN36345.1"
                     /translation="MSGQHFIRVGVLGSVGRFAAADRRRYARGTRVICRTARGLEVGE
                     VLSAVESPDADCDGSLLRRVSVQDDLLLARLEKNRDEAYRACASRLADSGQQAVLVDV
                     EHLFDGQSLFFYFLGETTPELDRLTAELAEVYEAKAQLQKFADTLTAGCGPHCGTEEG
                     GGCGTSGGCSTCAVGCGVKTERRRDGGTE"
     gene            complement(4020..4556)
                     /locus_tag="VFB80_21100"
     CDS             complement(4020..4556)
                     /locus_tag="VFB80_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZN36346.1"
                     /translation="MVDPQNTRHRPELVEPSAWIAPGAVVLGDVTLGTEATVWYGAVV
                     RGDSAPIRVGRQTNIQDGCILHADEGFPCTLGERVTLGHGSIVHGATVEDDCLIGMRA
                     VVMNGARIGKGSIVAVGSIVTEGTLIPPGSLALGQPAKVKRETSDRDRELIRHAAEHY
                     VAAGRVYRDAGKSDGGKE"
BASE COUNT          742 a         1511 c         1487 g          868 t
ORIGIN      
        1 atgtgcttgt cgctaaaaac cggcaccacg cggccgctct tctcgaagac cttgacgatc
       61 tcgccgaaca ttcgccgctt gggaaactga atgctgcccg tttcgctctc cgggtacttg
      121 ccgtgctcgg cgatgaggag cacgccatcg accgcgagct tctcgccgcc cagcgtgagc
      181 gccttctcaa ccgagtcata gatcgtgaag ccatgctcca tcgccagccg gcggctcatg
      241 tcgttcgccg ggaactgatc gatgtaggca ctgaccagct tgagcttggg aaactcccct
      301 ttgccgtcga ggttgtatcc ctgcaccagc cgcgacgcga tcacatccgc atgcgaattg
      361 tgccgccact cggtggtgac gacggcgacg gtgggaagct tgtcctgggc gagtgcggcc
      421 caggggaacg tcagcagcaa cagcgccgca gcgatggaga tcttgttccg aggcatggca
      481 atcctccttt gttctcacgg cccgcgcacg acgcgccgca cgtaagccac ttccacgacg
      541 cgatcgaatt cgcggaccgt aaaatactgc ctgagcaggc cgactgtcac ggcccgcagc
      601 ccctcggcca gcagctcccc tttgtgatgc gcgtcggtgg ccaggtattc gtcggcgagg
      661 tccgcggatt cggtcacttc ccgcttgtgg acggtcttca gccagaagcg ggccaggtcg
      721 tcgatcgccg actccagcca gacgaccgtg taccgcgtca ttgcgcggcc ttctcgttca
      781 gccgctcgag catctgtttc gtcgtataac gcggctcatt tgatgccaac cggcggcgga
      841 tctcggccag ttcctccgct gtcagctcgg gttcgaagcg gccgagcacc tcgccgcggg
      901 cattgagaaa gcggatgtca ccgccggatt cggcgagcgt tttgagaaag tcatcggaaa
      961 caacaatgtc gcgcattacg cgccgcctcc gggtcgaata ctcactaccg agtactgcgt
     1021 gcgcctttca ccataatacc acgctgtctg cgccaaccgc taacgttacc ccacgttcgt
     1081 ccacgccccg ctcttggcgc tggccagcac ggcgtcgcag atcttttggg tttcgagggc
     1141 gtcgcggaag gtggggccgg cgggctggtt cttcgcgagg ccgtcgagga agtcggcgac
     1201 ctggtggacg aagctgtgct cgtagccgat ggccaggccg ggcacccacc acttgcccat
     1261 gtagggatgc tcgccgtcgg tgacgtggat gctgctccag ccgcgaagcg ggccctcggt
     1321 gcggtggtcg aaccattgca ggcggtgcag gtcgtgcagg tcccacttga tcgaagcttt
     1381 ctcgccgttg atcccgaagg tgtagagggc tttgtgcccg cgtgcatatc gcgtgctttc
     1441 aaacaggccc agcgagccgt tcgtaaagcg gcagagaaac gcgcaggcgt cgtcgatgcc
     1501 gactttctcg accttcccag tcaggttgtg cttccgctcc ttgataaaag tctcggtcat
     1561 cgccgtgacg ttggcgacat tgccgttcag ccagatggcg gtgtcgatgc agtgggcgag
     1621 caagtcgccc gtcacgccgc tgccggctgc ggccacgtcc agccgccaga ggcccgcgcc
     1681 gccctggggc agatcggccg agatcgtcca gtcctgcaag aagttggccc ggtagtgaaa
     1741 gatcttgccg agcttgccgc tgtcgatgat ctgcttggcg aacgtcacgg ccggaatccg
     1801 gcggtagttg taccagacga tgttcgggac cttcgccttc tccacggcgc ggcacattac
     1861 ttcgccttcg gccgcgttca tcgccagcgg cttctcgcac aggatggcct tgcccgcctc
     1921 ggccgccgcg atggcgattt ccttgtggag gttgttcggc gtgcagatat cgaccgcgtc
     1981 gatgtccttc cgggcgagca gcttccgcca gtcggtctcg atcgactcgt agccccactt
     2041 gtcggcgaac ggtttcacct tcgccgcgtc gcgggcgcag gccgccttga gcaccggttg
     2101 atactccagg tcgaaaaagt tgcccacttt gcggtacgcg ttgctgtgcg cccggcccat
     2161 gaacccatag ccgatcatgc cgatgttgaa aggtcgcgcc atttgccaaa atcccttgct
     2221 gaaacggaag ccgccgctaa gccagaaacg tatcggaaga agagacccgc tgacaagcgg
     2281 cccgcggcgg gctgggatta cgaatgcccc gccggtactt cttcgtgccg cgtgacgacg
     2341 aagcccaact cgtcgatcat cctgtagtcc gcctccggcg gctggccctt cgtcgtcagg
     2401 taatcgccga cgaacatcga gttggcggca tacaggccga gggcctgcag gctgccgagg
     2461 tgcagctcgc ggccgccggc gatccgcagc tcgctccgcg ggttgacgaa ccggaacatg
     2521 gccagggcct tgaggcaata tcgcggattc agccggcggg aatgctccag cggcgtgccg
     2581 tcgatgtcga tcaggaagtt gagcgggatc gattccacag ccagctcgcg cagagccagc
     2641 gccatctgca ctacgtcgct gtcgcgctcg cccatgccga tgattccgcc gctgcacagt
     2701 tccagtcccg ccgcccgcac cgcattcagc gtctcgacgc ggtcctgata ggtatgcgtc
     2761 gtgcagatct ggccgtaaaa ctcggcgctg gtgttgaggt tgtggttcac cttgttcacg
     2821 ccgcaggccg ccagccggcg ggcctgctcg ggcgtgagca gccccaggca cgcgcagact
     2881 tcgaggtcgt gccgggcctt gatctcgggc acgatcttct cgaccgcctg gatctcgcgc
     2941 tcgctggggc cgcgggcgga aatcacgatg cagtacgtct tgctcccccg ctctgccgcc
     3001 agccgtgcgc cgtcgagcaa cttctcgcgg ctgaggaggt tgtagcgcgg aatgtcggac
     3061 gtggcggttt tggcctgcga gcagtagtgg caatcttctg ggcagaggcc gctcttggcg
     3121 ttcatcagga agtagagctg caccgtgttg ccgaaccagc ggcggcggat gcgataggcc
     3181 gcggccagca ggtccagcag ctcctcgtcc ggcgcacgga ggatggccag ggcctcgtcg
     3241 ttgttagctt gatggcccgc gaggacgcgc tcggccaggt ccgaccaggc tattgtggtc
     3301 gaagtctcgc tgagcggttc tacggcggtg tgaatcatcg agcgtccatc aggctaggca
     3361 gtcttgccgg gatcgatcaa gatgaaggaa gttcgttcgc gattctagct tcccagtgga
     3421 ttgcatgtca ggccagcatt tcatccgcgt cggcgtgctc ggcagcgtcg gccggtttgc
     3481 cgccgccgac cgccggcggt acgcgcgggg gacgcgggtg atctgccgca cggcgcgggg
     3541 attggaagtg ggcgaagtgc tctccgccgt cgagtcgccg gacgccgact gcgatggctc
     3601 gctgctgcgg cgcgtgtcgg tgcaagacga cctgctgctg gcccgccttg agaaaaaccg
     3661 cgacgaggcc taccgggcct gcgcctcgcg cctggccgat tcaggccagc aagccgtgct
     3721 ggtcgatgtc gagcatctct tcgacggcca atcgctgttc ttctatttcc tgggcgaaac
     3781 gacgcccgag ctggaccggc tcaccgctga gttggccgaa gtctatgagg ccaaagccca
     3841 gctccagaaa tttgccgaca cgctcaccgc cggctgcggc ccgcactgcg gcaccgagga
     3901 agggggcggc tgcgggacca gcggcgggtg cagcacgtgc gccgtggggt gcggggtgaa
     3961 gacggagaga cggagagacg gagggacgga gtgaaggagc gaggctcgcc gtctctccgt
     4021 cactccttcc ctccgtcact ctttcccgcg tcccgataca ccctccccgc cgccacataa
     4081 tgctccgcgg cgtggcgaat cagctcgcgg tcgcggtcgc tggtttcgcg cttgaccttc
     4141 gccggctggc cgagggcgag gctgcccggc ggaatcaggg tgccttcggt cacgatgctg
     4201 cccacggcca cgatgctgcc cttgccgatt cgcgctccgt tcatcaccac ggcccgcatg
     4261 ccaatcaggc agtcgtcctc gaccgtcgcg ccgtgcacga ttgagccgtg gcccagcgtc
     4321 acccgctcgc cgagcgtgca agggaaacct tcgtcggcgt gcaggatgca gccgtcctga
     4381 atgttcgtct gccggccgac gcggatcggc gccgagtcgc cgcgaaccac ggccccgtac
     4441 cagaccgtcg cctccgttcc cagcgtcaca tcgccgagca cgaccgcacc cggcgcgatc
     4501 caggccgacg gctcgaccag ctcggggcgg tggcgggtgt tttggggatc gaccacggtg
     4561 taccttactt cgcatgatga ccgaactgcc gagcatcgcc aaggcggc
//
LOCUS       DASRJB010000805         7501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4188063, whole genome shotgun sequence.
ACCESSION   DASRJB010000805 DASRJB010000000
VERSION     DASRJB010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188063"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1865
                     /locus_tag="VFB80_21105"
     CDS             <1..1865
                     /locus_tag="VFB80_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36347.1"
                     /translation="FDCANPRNSGGASNRDELKAKDAPLAALLTEVYGDTPWRYVKTE
                     RRKAAPDTAHLAGMDRAAYPVFSFSNSPRIKAEAEAARQPARPEPVKQPTAAAIEQVE
                     QGPRPPAEIVASFDGLGEGFEGPQGKSRGRNPSDNSLAVGPNHIVQIVNSQMAVFTKA
                     GEKFDATGKVLYGPVSTNNVFRGFAGEAAQMNNGDAVVRYDQLADRWLIVMPIFRRLP
                     PKEAEPPMPKAGEVAHCSLASVANQPGPAELLFQPPAPADTAAGARRAAPRPAAGMRG
                     SYAMCYAISTSSDPLGSYYRYQFVRPLFPDYPRPAVWPDGYYVPTSTGDDVVQKHIYV
                     VEREKMLKGEPAREQGIIIDDVNFLNCADLDGKQLPPAGAPNLVLAAGGTQLKNVLQD
                     DGIYAWKLSVNWDDASKTRLDGPAKIPVAPYEYLGGGQLTRTVPQPGTDVRLDSQGDK
                     LMARLVYRRIGDRESLVAVHSVNTGAGGGGVRWYEFRLDGQRNVALHQQGTYCPEGSY
                     RWMASPAIDGQGNIGIGYSFGDKDHFPGQRFAARREGDPLGVLSLREAVMAEGQAAQT
                     NTFRWEDYSQTAVDPSDDRTIWYVGDYLKAGATNYSTRLAAFRIGDGRGEQPAR"
     gene            complement(1879..3012)
                     /locus_tag="VFB80_21110"
     CDS             complement(1879..3012)
                     /locus_tag="VFB80_21110"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN36348.1"
                     /translation="MPDPHTLARWRLVLGKAAESHGISLEGQAEAQRIEQLVGFLFEP
                     GGEGQESGGRSSDRSAGRGPSQLTVPSWVDAVNELFPQQSKEVMQKELVRRRGIAELM
                     EKPELLEKIEPNLELVKTLLTHRELLNPKTRILARKIIDKVVQELKRKMQVQVEQAIT
                     GAIRKDRHSPRKVFRNLDLKTTLRRNLQHFDSASGKLHVDRLFFFAAERKKKPWHVIV
                     VVDQSGSMLESAIFSAVMASIFAELPAVRTSLVLFDTQVVDLSDKVGQPVDVLLSIQL
                     GGGTDITQALIYASELVRQPQRTIVVLITDFYEGRAESDLVAQTRQMADSGLRMIGLG
                     ALGYDARPSYNKATAGKLRKVGMDILVCTPEKLAECMAQIIRG"
     gene            complement(3031..5436)
                     /locus_tag="VFB80_21115"
     CDS             complement(3031..5436)
                     /locus_tag="VFB80_21115"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HZN36349.1"
                     /translation="MKGVNSQAAGLDPEAVARQVESVLAEPLYWFGVRHHSHTVARHL
                     ASAMQSRRPKLVFIEGPHEANDLIPFVVDARTEPPVAIYSSYRDDDNVLGLSGVLSPA
                     KEIPARFAAWYPLTAYSPEYVAMKTAAALGADVVFIDLPHYARIKPDDDDAKRPPPAT
                     DDDALITASGFYQQLAAAAGFKTWDEAWDTLFENPHAADHEVFRREMATFCCAVRLTS
                     NPATEAAQGTLERERHFAKVIRETLADRKLKPEQAMVVCGGFHLFLDRNDAAPPPSLP
                     AGTVYTTVVPYSYFRVSEMAGYGAGNRAPQYYQTCFDLVAAGRQHDIALEHAIAVLRQ
                     MRKEGEPLSTADAIAITHHAGLLASLRGRSHTTLDDIDDALVTCCCKGDPGDEGLKLR
                     AAMDAAGIGNRIGKVTPKLGRLPIVSDFHAQLADLDLGQVLGKEKRLAVRLDKREPLA
                     ARRSAFFHRLAFLEIPFAALSSTGGDFSGTIFREDWQLKWDPKTEPALIEQNLYGDSV
                     EAAAVNRLREAIAAAGANAGTTCERLLRAVNMDLPDLVQIAEAAAGQAFDGDPAFPSL
                     AAALKHLAMLDRYASFRGTRRETIEGLLVRCYDRACFALPNSAAVPDEEQPDVVDGLV
                     CVADILQRAQPEQYDRALFSAATRRAAEESPVPFLRGAFLGLLCEIRELPAESLAAEV
                     AGLAGAAPDLMVTAGDLLDGMLAVSRTSIMLGADALVGAVDDLLKAAAWEPFLVMLPR
                     LRAAFERLAKSHRDSLAACVARRYGLGSAAEVQTVRSSLGATALVARLDAAVAAALRE
                     WKL"
     gene            complement(5460..6611)
                     /locus_tag="VFB80_21120"
     CDS             complement(5460..6611)
                     /locus_tag="VFB80_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN36350.1"
                     /translation="MAKKTEATAQPAASEVLREPAEVRYADQLEALKQNDTETPPPNW
                     RLSPRSVLLYVLGGKTLKAKIDGKAVEVPITRKFFGDDGLVERAIVTLASERALLLIG
                     EPGTGKSWLSEHLAAAVSGTSILTIQGTAGTTEEHIKYSWNIARVIAEGPRPDNLIPS
                     PTMVAMRCGGLLRFEELTRCVPDVQDALVSILSDKAIAVPELPDNNMVWARPGFNVIA
                     TANTRDQGVNELSAALKRRFNYIYIPIVGDQKTEVKIVQQRSSELLARYNLPAKLTPP
                     VIELLATVFREIRSGKTSDGVSLKKPTTTLSTAEAIGVALDAALHAQFFGGGEIGPAD
                     IARNMVGVVVKEDQEDRDSLKEYAALVAKKRGAKEKSWKEFHDALVEQL"
     gene            complement(6631..>7501)
                     /locus_tag="VFB80_21125"
     CDS             complement(6631..>7501)
                     /locus_tag="VFB80_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36351.1"
                     /translation="TFFLSRAWLLSRGLAQALQAADEKQYDRLTWSPPAQPLERVEVV
                     CLGVAKRVAAGAFVAFDFRFRAIGGAAPVKPGQSLSWSFVFPLKPGLEIPPEGFLHLP
                     QKQKFTPFILLDRKSVVVERANLTADETGGRLTLTDQSTVVLGKAFDEWEQFLEWSPE
                     PAVARIGKHIPGPLDLDTELQEEIVLRDYAIGRPEEGDAPGQTVYPIAAGPLKLTAIV
                     GSPAEGKALRKSLDELRKLKKDRPPLYGLMHYERSRLVLQVLTAFRPEPDYLTISKEN
                     VNKAALLKAMSFT"
BASE COUNT         1278 a         2480 c         2462 g         1281 t
ORIGIN      
        1 ggttcgattg cgccaacccg cgcaacagcg gtggggccag caaccgcgac gagctgaagg
       61 ccaaggacgc gccgctggcg gccttgctca ccgaggtgta cggcgacacg ccctggcgat
      121 acgtcaagac cgagcgccgc aaggcggccc ccgacacggc gcacctggcc ggcatggatc
      181 gcgcggctta cccggtcttc agcttcagca attcgccgcg gatcaaggcc gaggccgaag
      241 ccgcgcggca gccggccagg ccggagcccg tcaagcagcc gacggcggcg gcgatcgagc
      301 aagtagagca gggacctcgg ccgccggccg agatcgttgc cagcttcgac ggcctcggcg
      361 agggcttcga agggccgcaa gggaaaagtc gcgggcgcaa tccatcagac aattcgctgg
      421 ccgtcgggcc gaaccacatc gtgcagatcg tcaacagcca gatggccgtg ttcacgaaag
      481 cgggcgagaa attcgacgcc accggcaagg ttctctatgg cccggtctcg acgaacaacg
      541 tcttccgcgg ctttgccggc gaagccgccc agatgaacaa cggcgacgcc gtcgtgcgct
      601 acgatcagct cgccgaccgc tggctgatcg tcatgccgat ttttcgccga ctgccgccca
      661 aggaggccga gccgcccatg ccgaaggccg gagaagttgc gcactgcagt ctggcgagcg
      721 tcgcgaatca gcccgggcct gccgagctcc tgttccagcc gccggcgccc gcggacaccg
      781 ccgctggcgc gcgacgggcc gccccgcgtc cggctgccgg aatgcgggga tcgtacgcca
      841 tgtgctacgc gatcagcacc agctccgatc cgctgggttc gtactatcgc taccagttcg
      901 tccgcccgct gtttccggat tacccgcggc cggcggtctg gcccgacggc tactacgtgc
      961 cgacgagcac cggcgacgac gtcgtgcaga agcacatcta cgtcgtcgag cgcgaaaaga
     1021 tgctgaaggg tgagccggcc cgcgagcaag gcatcattat cgacgacgtc aacttcctga
     1081 actgtgcgga cctcgacggc aagcagctgc cgcccgccgg agcaccgaac ctggtcctcg
     1141 ccgcgggcgg cacacagctc aagaatgtcc tgcaggacga cggcatctac gcctggaagc
     1201 tgtcagtcaa ttgggacgac gcgtccaaga cgcgcctcga tggcccggcg aagattcccg
     1261 tcgctccgta cgagtatctg ggcggcggcc agttgacgcg caccgtcccg cagccgggaa
     1321 ccgacgtccg gctcgattcg cagggggaca agctgatggc ccggctggtg taccggcgga
     1381 tcggcgaccg cgaatcgctg gtggccgtcc attcggtgaa taccggagcg ggcggcggtg
     1441 gcgttcgctg gtacgaattc cgcttggacg gccagcgcaa tgtcgcactg caccagcagg
     1501 gcacttattg cccagaggga agctatcgct ggatggcgag tccggccatc gacgggcagg
     1561 ggaacatcgg cattggctac tccttcggcg acaaagacca ttttccgggc cagcgatttg
     1621 ccgcacggcg tgagggtgat ccgctcggcg tcctatcgct gcgcgaagcc gtgatggccg
     1681 agggccaggc ggcccagacg aacacgtttc gctgggaaga ctattcccag accgccgtgg
     1741 acccatccga cgaccgcacg atctggtacg tcggcgacta cttgaaggcc ggggccacga
     1801 attacagcac ccgcctcgcg gcatttcgca ttggcgatgg ccgcggggaa caaccagcca
     1861 ggtaggcgaa ccgatggctc aacctcgaat gatctgggcc atgcattcgg ccagcttttc
     1921 cggcgtgcaa accagaatat ccatgcccac tttgcgaagc tttccggccg tcgccttgtt
     1981 gtacgagggg cgggcgtcgt aacccagagc gccgagcccg atcatccgca ggccgctgtc
     2041 ggccatttgc cgcgtctggg ccacgaggtc gctttccgcc cgcccttcgt aaaaatcggt
     2101 gatgagcacc acgattgtgc gctgcggctg gcggaccagc tcgctggcgt aaatcaaggc
     2161 ctgcgtgatg tccgtgccgc cgccgagctg gatcgacagc aggacgtcca cgggctggcc
     2221 gactttgtcg gacaaatcga ccacttgcgt atcgaacagc accagcgacg tcctgaccgc
     2281 gggaagttcc gcaaagatcg aggccatcac cgccgagaag atcgcgctct ccagcatcga
     2341 gccggactgg tccacaacga caatcacatg ccaaggcttc ttcttccgtt cggccgcaaa
     2401 gaagaacagc cgatcgacgt gcagcttgcc cgaagcggag tcgaagtgct ggaggttgcg
     2461 gcggagcgtt gttttcaggt ccagattgcg gaagaccttg cgcggcgagt ggcggtcttt
     2521 gcggatcgcg ccggtgatgg cctgctccac ctgcacctgc attttacgct tcaattcctg
     2581 cacgaccttg tcgatgatct tgcgggccag gatgcgggtc ttgggattga gcagctcgcg
     2641 gtgcgtgagc agcgttttga ccagctccag gttgggctcg atcttctcca gcagctctgg
     2701 tttttccatg agctcggcga tgccgcgccg ccgcacgagc tccttctgca tcacctcctt
     2761 cgactgctgc gggaacagct cgttgacggc atccacccag ctgggaactg tcaactgcga
     2821 gggcccccgg ccggccgagc ggtcggacga ccggccgccg ctttcctggc cctcgccacc
     2881 cggttcaaag aggaagccga cgagctgctc gatccgctgg gcttcggctt gcccctccag
     2941 ggaaatgccg tgcgactccg cggccttgcc gagcacgagc cgccagcggg cgagcgtgtg
     3001 aggatctggc atttcggcaa gccctccgaa ttacagcttc cattcccgaa gtgccgccgc
     3061 gaccgccgca tccagccggg ccacgagcgc ggtcgccccc aggctgctgc ggacggtctg
     3121 cacttcggcg gcggagccga ggccgtagcg tctggcaacg caggccgcca gcgagtcgcg
     3181 gtgggacttc gccagccgct cgaacgccgc ccgcagccgc gggagcatga ccaggaacgg
     3241 ctcccaggcg gcggctttca gcaggtcatc gacggcgccg accagcgcgt ctgcgccgag
     3301 catgatggaa gtccgcgaga cggcgagcat cccgtcgagc aagtcgccgg cggtgaccat
     3361 caggtcgggt gcagcgccgg ccagcccggc cacttccgcg gcgagcgatt cggcaggcag
     3421 ctcgcgaatt tcacacaaca ggcccaggaa agccccgcgc aggaatggca cgggcgactc
     3481 ttcggccgcc cgccgcgtcg ccgcggaaaa cagcgcccgg tcgtactgtt caggctgcgc
     3541 ccgttgcagg atgtccgcga cacaaaccag cccgtccacg acgtccggct gttcctcgtc
     3601 cggcacggcc gcggagttgg gcaaggcaaa gcaggcgcga tcgtagcagc ggacgagcag
     3661 cccctcgatc gtttcccgcc gcgtgccccg gaacgaggcg tagcggtcga gcatcgccag
     3721 gtgcttgagc gccgcagcga gcgacggaaa cgcggggtcg ccgtcgaacg cctggcccgc
     3781 ggccgcttcg gcgatctgca ccaggtcggg caggtccata ttcacggccc gcagcagccg
     3841 ctcgcaggtt gtgccggcat tcgcgcccgc tgcggcgatc gcctcccgca agcggttgac
     3901 cgccgccgct tcgacgctgt cgccgtagag gttctgctcg atcaatgccg gctcggtttt
     3961 gggatcccac ttgagctgcc agtcctcgcg gaagatcgtc ccggaaaagt cgccgccggt
     4021 gctcgacagc gcggcgaagg ggatttccag aaatgccagg cgatggaaaa acgccgagcg
     4081 gcgggcggcc agcggttcgc gcttgtcgag cctcaccgcg agccgttttt ccttcccgag
     4141 cacctgcccc agatcgagat cggcgagctg cgcgtgaaaa tcgctgacga tcggcagccg
     4201 gccgagcttg ggcgtgactt tgccgattcg attgccaatg ccggcggcgt ccatcgcggc
     4261 gcggagcttg agcccttcat cgcccggatc tcccttgcag cagcaggtca cgagggcgtc
     4321 gtcgatgtcg tcgagcgtgg tgtggctgcg gccgcggagg ctcgcgagca aaccggcatg
     4381 gtgcgtaatc gcaatcgcat cggcggtgga gagcggctcg ccctccttac gcatctgccg
     4441 caagacggcg atcgcgtgtt ccagcgcgat gtcgtgctgt cgcccggcgg cgacgagatc
     4501 gaagcaggtc tggtaatact gcggggcgcg attgccggcg ccgtagccgg ccatctccga
     4561 gacgcggaag tacgaataag gcacgaccgt ggtgtagacg gtgcccgccg gcaagctggg
     4621 gggcggtgcg gcatcgttgc ggtcgagaaa caggtgaaac ccgccgcaaa ccaccatcgc
     4681 ctgctcgggc ttgagcttgc ggtccgccag cgtttcgcga attaccttcg cgaagtgccg
     4741 ctcccgctcg agggtgcctt gggccgcttc cgtggccgga ttggaagtca gccgcaccgc
     4801 gcagcaaaac gtggccattt cgcgacgaaa gacttcgtgg tccgcagcgt gcggattctc
     4861 gaacagcgtg tcccaagctt catcccaggt cttgaatccc gcggcagccg ccaactgttg
     4921 atagaagccg ctcgcagtga tcagcgcgtc atcgtcggtc gccggcggcg gccgtttcgc
     4981 atcgtcatcg tcaggtttga tccgcgcgta gtgaggcaag tcgatgaaca cgacatcggc
     5041 ccccagtgcg gcggccgttt tcatcgccac gtattcaggc gaatacgccg tgagcggata
     5101 ccaggcggca aatcgcgcgg gaatctcttt cgccggactg aggacgccgc tcaagcccag
     5161 gacattatcg tcgtcccggt acgacgagta gatcgccacc ggtggctcgg tcctggcgtc
     5221 gactacgaat ggaatgagat cgttggcctc gtgcgggccc tcgatgaaga ccagcttggg
     5281 ccggcggctc tgcatggcgc tggccaggtg ccgcgcgacg gtgtgcgaat ggtgccgcac
     5341 gccgaaccag tacagcggct cggccagcac gctctcgacc tggcgcgcca cggcttctgg
     5401 atcgaggccc gcggcttggg aattgacgcc tttcatggca gagatcgtcc gcggccggct
     5461 tacagttgtt cgacgagcgc gtcgtggaat tccttccagg acttttcctt cgcgccgcgc
     5521 ttcttcgcca ccagggccgc gtactctttc agcgagtcgc ggtcctcctg gtcctctttg
     5581 acgactacgc ccaccatgtt gcgggcgata tccgccgggc cgatctcgcc gccgccgaag
     5641 aactgggcgt gcaatgccgc atcgagggcc acgccaatcg cctccgcggt ggagagcgtc
     5701 gtggtcggct tcttcaggct cacgccgtcg gacgtcttgc cgcttcggat ctcgcggaag
     5761 accgtcgcca agagctcgat caccggcggc gtcagcttcg ccggcaggtt gtaccgcgcc
     5821 agcagctcgc tggaccgctg ctgcacgatc ttcacttccg ttttctggtc gccgacgatc
     5881 gggatataga tgtaattgaa gcggcgcttg agggccgccg agagctcgtt cacaccttgg
     5941 tcgcgggtgt tggccgtagc gatcacgttg aaaccgggac gggcccagac catgttgttg
     6001 tcgggcagtt cgggcacggc gatcgccttg tccgagagaa tcgacactag cgcgtcctgc
     6061 acgtcgggca cgcagcgggt cagctcttcg aaccgcagca ggccgccgca gcgcattgcg
     6121 accatcgtgg gcgaggggat caggttgtcc ggacgcggcc cctcggcgat gacacgggcg
     6181 atgttccagg aatacttgat gtgctcctcg gtcgtgccgg ccgtcccctg gatcgtgaga
     6241 atcgacgtgc cggagactgc cgcggccagg tgctcggaga gccagctttt gcccgtaccc
     6301 ggctccccga ttagcagcag agcccgctcg gaggcgagcg tcacgatggc ccgctcgacg
     6361 aggccgtcgt cgccgaagaa tttgcgcgtg attggcacct cgaccgcctt gccgtcgatc
     6421 ttggccttga gcgtttttcc gccgaggacg tagagcagca ccgaccgcgg cgacaagcgc
     6481 cagttcggtg gcggtgtttc ggtgtcgttc tgcttcagcg cttcgagctg gtccgcgtat
     6541 cgcacctcgg ccggttcgcg gagcacttcg cttgccgccg gctgagcagt ggcttcggtc
     6601 tttttggcca tgatgaatcc caatcggact tcaggtaaac gacatcgcct tgagcaaggc
     6661 cgccttgttc acgttctctt tggaaatggt gaggtaatcg ggctcggggc ggaaggcagt
     6721 cagcacttgc agaaccagcc gcgagcgctc gtagtgcatc agcccataca gcggcggacg
     6781 atctttcttg agcttgcgca attcgtcgag actcttgcgc agggctttgc cttcggccgg
     6841 cgaaccgaca atcgccgtga gcttgagcgg acccgccgcg atcggataga ccgtctggcc
     6901 gggcgcatcg ccttcttcgg gccggccgat cgcgtagtcg cggagcacga tctcttcctg
     6961 caattcggtg tccaggtcaa gcgggccggg aatatgcttg ccaatccgcg ccacggctgg
     7021 ctcgggcgac cattcgagga actgttccca ctcgtcgaac gccttgccga gcaccaccgt
     7081 actctgatcc gtcaacgtga gccggccgcc ggtctcgtcg gcggtgagat tggcccgttc
     7141 gacgaccacc gacttccggt cgagcaaaat gaagggcgtg aacttctgct tctgcggcag
     7201 gtgcaagaag ccctccggcg gaatctccag accgggcttg agcgggaaca cgaacgacca
     7261 gctgagcgac tggccgggct tcaccggcgc tgcaccgccg atagcccgaa accggaagtc
     7321 gaacgccacg aaggccccgg ccgcaacccg cttggccacg cccaggcaaa cgacctcgac
     7381 gcgttccagc ggctgcgcag gcggcgacca ggtcagccgg tcgtattgct tctcgtcggc
     7441 cgcttgcagc gcttgcgcga gcccgcggct gagcagccag gcgcggctaa ggaagaacgt
     7501 c
//
LOCUS       DASRJB010000806         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_797568, whole genome shotgun sequence.
ACCESSION   DASRJB010000806 DASRJB010000000
VERSION     DASRJB010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797568"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFB80_21130"
     CDS             <1..1177
                     /locus_tag="VFB80_21130"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN36352.1"
                     /translation="CRGPADALPCLSVRSGFDLLLRALDLPRKSEVLLSAVTIPDMAR
                     IVEAHGLVAVPVDLDLLALGPLPAALEAAVTPRSRLLVVAHLLGSRVESAPLVEFARR
                     HGLLLIDDLAQGFAGWPEPCAADVALYSFGPIKTATALGGALVRVRSAGLLARLQEIQ
                     ASDPLQPRLAFAWRLLKFAALKAASARPAFDLLVGLLRAAGCEYDRLFAGLFRGFPGP
                     DLLGKLRQRPSAALLSLLARRLEHFDVGRLDDRAAQARRLIGRIAGRWFVPGAAAWEH
                     THWAVPVLAADPPRLIQRLARAGFQASQPRSLTAVAPPADRPQLTPRQAQRLIAEAVF
                     LPVYRELPAREIERLAQVLLSCAEFASPPPACWAVSPSRPPPRPSETCALRGGWRGP"
     gene            complement(1107..1469)
                     /locus_tag="VFB80_21135"
     CDS             complement(1107..1469)
                     /locus_tag="VFB80_21135"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZN36353.1"
                     /translation="MFLFEHRDEPLVSPRRFYWRMARAFGVTLLIVAGSLAMGVAGYH
                     YINRLPWIDALYNSAMILTGMGPADAPRTAAGKLFATFYALYSGIAFLSMVAVLMAPA
                     IHRAMHKFHLDVEEDERD"
     gene            complement(1494..2225)
                     /locus_tag="VFB80_21140"
     CDS             complement(1494..2225)
                     /locus_tag="VFB80_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN36354.1"
                     /translation="MSAKHILVVEDERHLAMGIKFNLEAEGFRVTTVGDGPAALHLIE
                     EGDADVDLVVLDIMLPGMSGYAVCERIREQGSDLPVLILSARTLSEDRTRGFDVGANQ
                     YLSKPFELDELLARVKNLLTHHARRHQSARVVPSTLGKFEFAAAQIDFPQYELKVGGQ
                     SVQLTKREWELLEYFVENEGRLIPRQELLEKVWRMPGHIQTRAPDQFILRLRKVFEAD
                     SANPKHFLTIRDMGYRFVARPEEAA"
     gene            complement(2257..>3293)
                     /locus_tag="VFB80_21145"
     CDS             complement(2257..>3293)
                     /locus_tag="VFB80_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZN36355.1"
                     /translation="THWTDGFLGGQMWQFHLRTKDSWWRKQAEHYSKLLEDRQHDRNV
                     HDLGFIFLSTYLPWYELTGDDQLHGVLIQAGRTLAMRFKEKGQYLRSFVSDDSLFIDI
                     MMNVPIIFYAAIETGDRELARVATAHCRTTRDKIVRADGSTAHEGIFDLQTGTFLRED
                     THQGLAPNSAWARGLAWSLYGYGKCFALTGDSAWLAVAERNARYWIDHLPADGVPYWD
                     FHADLSQPPPWGAQKESSAGAIAASGLLDLARLTRDPARRSEYEQTALKMLDAMVQPE
                     YLASQTPGWEGILKHGVYHTKKNLGVDESVMWGEYFFVEALTKVASNQYGRLAPEVLS
                     RNREKFAAVS"
BASE COUNT          476 a         1109 c         1134 g          574 t
ORIGIN      
        1 ctgccgcggc ccggcggacg cactgccctg cctctcggtc cgcagcggct tcgacctgct
       61 gctgcgggcg ctagacctgc cgcggaaatc ggaagtgctg ctgtcggcgg tgacgatccc
      121 cgacatggcg cggatcgtgg aggcgcacgg actggttgcc gttcccgtcg atctcgacct
      181 gctggcgctc gggccgctgc ccgccgcgct ggaggcggcc gtcactccgc gctcgcgact
      241 gctcgtcgtc gcccacctgc tgggttcgcg cgtggagagt gctccgctgg tggagttcgc
      301 acgccggcac ggcctgttgc tcatcgatga cctggcccag ggattcgccg gctggcccga
      361 gccctgcgcg gccgatgtgg ccctttacag ctttggaccc atcaagacgg cgacggccct
      421 gggcggcgcg ctcgtgcggg tccgctcggc cggcctgctg gcccgcctgc aggaaatcca
      481 ggcctccgat ccgctgcagc cgcgattggc atttgcctgg cggctcttga agttcgccgc
      541 gcttaaggcg gccagcgcac ggccggcttt cgacctgctc gtcgggctat tgcgggcggc
      601 cggctgcgaa tacgaccggc tcttcgcggg cctgttccgc ggcttccccg ggccggattt
      661 gcttggcaag ctgcgccagc gcccgtcggc cgcactgctg tcgttgctgg cccgccgcct
      721 ggagcacttc gacgtcggcc ggctggacga ccgcgcggcc caggcccgcc ggctgatcgg
      781 cagaatcgca ggtcgctggt tcgttcccgg cgcggccgct tgggagcaca cgcactgggc
      841 ggtgcccgtg ctcgccgccg atccgccgcg gctgatccag cgactggctc gcgccggctt
      901 tcaggcgtcg caaccccgca gcctcaccgc cgtcgcgccg cccgcggacc ggccgcagct
      961 gacgcctcgc caggcgcagc ggctcatcgc ggaggcggtt ttcttgcccg tctatcgcga
     1021 gctgcccgcg cgggaaatcg agcggctggc gcaggtgctg ctgtcctgcg cggaattcgc
     1081 ctcgccgccg ccagcctgct gggcagtcag tccctctcgt cctcctccac gtccaagtga
     1141 aacttgtgca ttgcgcggtg gatggcgggg gccatgagga cggcgaccat cgagagaaag
     1201 gcgatgccgc tgtagagggc gtagaacgtg gcgaacagct tgccggcggc ggtccgcggg
     1261 gcatcggccg ggcccatgcc ggtcaggatc atcgccgagt tgtaaagcgc gtcgatccac
     1321 ggcaggcggt tgatgtagtg atagccggcc acgcccattg ccagcgagcc cgccacgatg
     1381 agcagcgtca cgccgaacgc ccgggccatc cgccaataaa accgccgcgg gctgacgagc
     1441 ggctcgtccc ggtgctcgaa gaggaacatg cggcgtgctc cagacgcagc cggtcaggcc
     1501 gcttcttccg gccgggcgac gaagcggtag cccatgtcgc ggatcgtgag gaagtgcttg
     1561 gggttggccg agtcggcctc gaagaccttg cggaggcgga ggatgaactg gtcgggggcc
     1621 cgcgtctgga tgtggccggg catccgccag accttctcca gcagctcctg ccgcgggatc
     1681 agccggcctt cgttctcgac gaagtactca agcagctccc actcccgctt ggtgagctgc
     1741 acgctctgac cgccgacctt cagctcatac tgtgggaagt cgatctgggc ggcggcgaac
     1801 tcgaacttgc ccagcgtgct gggcacgacc cgcgcggact ggtgccgccg ggcgtggtgc
     1861 gtgagcaggt ttttcacgcg ggccagcagc tcgtccagct cgaacggctt gctcagatac
     1921 tggttggccc cgacgtcgaa gccccgcgtg cggtcctccg agagcgtgcg ggccgacagg
     1981 atcagcaccg gcaagtcgct cccctgctcg cggattcgct cgcagaccgc gtagccgctc
     2041 atgcccggga gcatgatgtc gagcacgacc agatcgacgt cggcatcgcc ttcctcgatc
     2101 aggtgcaggg ccgccggccc atcgccgacc gtcgtgacgc gaaagccctc ggcctcgagg
     2161 ttgaacttga tccccatcgc cagatggcgc tcatcttcga cgacgaggat gtgcttggca
     2221 gacatggccg gggatgggga gccggggggt cgggagtcag gagacggcgg cgaacttttc
     2281 tcggttgcgg gagaggactt cgggcgcgag gcggccgtac tggttgctgg cgactttggt
     2341 cagggcctcg acgaagaagt actcgcccca cattaccgac tcatcgacgc ccaggttctt
     2401 cttcgtgtga tagacgccgt gcttgaggat cccttcccag ccgggcgtct ggctggccag
     2461 gtactcgggc tgcaccatcg catcgagcat cttcagggcc gtctgctcgt actcgctgcg
     2521 gcgggccgga tcgcgggtca gccgggccag gtccagcagg ccgctggcgg cgatcgcgcc
     2581 ggcgctcgac tctttctgcg cgccccaagg cggcggctgc gacagatcgg cgtggaaatc
     2641 ccagtacggc acgccatcgg cgggcaagtg gtcgatccaa tagcgggcgt tccgctcggc
     2701 gactgccagc cacgcggaat cgccggttag cgcgaagcac ttgccgtagc cgtagagcga
     2761 ccaggccagg ccgcgggccc aggcgctatt cggcgccagg ccctggtgcg tgtcttcgcg
     2821 gaggaacgtg ccggtctgca agtcaaaaat gccttcgtgg gcggtggagc catcggcccg
     2881 gacgatcttg tcccgcgtcg tgcggcagtg ggccgtggcg acgcgggcca gctcgcggtc
     2941 gcccgtctcg atcgccgcgt aaaaaatgat cggcacattc atcatgatgt cgatgaacag
     3001 cgaatcgtcg ctgacgaacg accgcagata ttgccccttt tccttgaacc gcatcgccag
     3061 tgtccgcccg gcctgaatca gcacgccgtg cagttggtcg tcgcccgtca gctcgtacca
     3121 gggcaaatac gtgctgagga agatgaatcc caggtcatgc acgttgcggt cgtgctggcg
     3181 gtcttcgagg agttttgaat agtgctcggc ctgttttcgc caccaggaat ctttcgtgcg
     3241 gagatggaat tgccacatct gaccgccgag gaagccgtcg gtccaatgcg tcc
//
LOCUS       DASRJB010000807         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_238222, whole genome shotgun sequence.
ACCESSION   DASRJB010000807 DASRJB010000000
VERSION     DASRJB010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238222"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFB80_21150"
     CDS             complement(<1..666)
                     /locus_tag="VFB80_21150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZN36356.1"
                     /translation="MSRNSLVALWIAAALTANVAFAQDRALSPDEAVASWKLPPGFQA
                     TLFAAEPEITQPMAFTFDDRGRVWVVECKSYPTWREDGTGNDRVTILEDTNGDGRHDK
                     RTVFFDKGANLSGIEWGFGGVWLTAVPNLIFIPDRDGDDRPDAGPRVLLDGWDLKARH
                     NVVGNLAWGPDGWLYGCNGILSNSLLGKPGTPPDRRTPMNCGVWRYHPLRHEFEAYAH
                     GTTN"
     gene            957..>2653
                     /locus_tag="VFB80_21155"
     CDS             957..>2653
                     /locus_tag="VFB80_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN36357.1"
                     /translation="MELPSRAKGNILLAPGEMLALRERLRRMAPQHDLATVIACAFDH
                     RTRILPFIYADMRMPPAGVRAIGSALVDAGFPKTRIVLQQWNQNFRPSEMRLDGRIPD
                     LFCVSSMHLHGSECDRLIRDCQRIDPARRPLIVVGGPRMVYEPWLAFGTDADNPWGAD
                     VAVTGEEFVFLSLLEVLLSLRAQGEPLRSVFLRARDSGALDEIPGLVYARQSRPDGPA
                     EELVDTGIQRLLGDLDELPHPVLGYQLLEAPSRKSTLASSALPAKRVRRYSAISSIVL
                     TVGCKFRCAYCPIPAYNQRQHRTKSGERIADEMGRITSTYGISFFFGTDDNFFNETKR
                     TLEIAETLARKAAERKRPHCKILYGTEATVHDTIRMQEHLPLIRRSGMAAVWMGVEDL
                     TGTLVKKGQNQSKTIEAFQLLRASGIAPVPMMMHHDSQPLVTLKSNYGLLNQLRTLRK
                     AGAMYTQVLMLTPAPGSKWYEDTYTSGLAFDAVGGEPLAPHIVDGNYVVASKHARPWV
                     KQLNLLIAYLYFFNPLRTLLALAFSLSRIPLVDADNRPAAEIARYSPLARIARTLYL"
BASE COUNT          457 a          909 c          807 g          480 t
ORIGIN      
        1 gtttgtcgtc ccatgggcgt aggcctcgaa ctcatggcgc agcgggtgat agcgccatac
       61 gccgcagttc atcggggtgc ggcggtcggg cggtgtgccc ggcttgccca gcagcgagtt
      121 gctgagaatc ccgttgcagc cgtagagcca gccgtcgggc ccccaagcca gattgccgac
      181 gacgttgtgc cgcgctttca gatcccaacc gtcgagcaga acccgcggcc cagcgtccgg
      241 ccgatcgtcg ccgtcacgat cggggatgaa aatcaggttg ggaaccgccg tcagccagac
      301 cccgccgaat ccccattcga tgccgctgag gtttgcgcct ttatcgaaga aaaccgtccg
      361 cttgtcgtgc cggccgtcgc cgttggtgtc ttccaggatc gtcacgcggt cgttgcctgt
      421 gccgtcctct cgccaggtcg gatacgactt gcactccacg acccagactc gcccgcgatc
      481 gtcgaaagtg aacgccatcg gctgggtgat ttccggctcc gcggcaaaca gcgtggcctg
      541 aaatcccggc ggcaacttcc agctcgccac cgcttcgtcc ggcgatagcg cgcgatcctg
      601 agcgaacgcg acgttcgcag tcagggccgc cgcgatccac agtgctacca gactgtttct
      661 gctcatacgt gcctccggga tttcagcgag cgttacatcg taacgtgata gcacgtggtc
      721 cgcacaaatc ggcccgggcg gtggtgcgga tctcgtgcaa attccgatga tgaccgaggc
      781 tgcccaattc gcgtggctgc gccgccaatt tgcggcggcc tgggaaatct gggcaaactt
      841 gtcgcccaca attcggcgtg gcgtcaaatc cgcctttctg cgacgattcg cgccccgctc
      901 ttgcctagac cgctggcagc cggccttccc gcccagcttg ccacggagac ccattgatgg
      961 aactgcccag tcgcgccaag ggaaacatcc tgctcgcccc cggcgagatg ctcgccctcc
     1021 gcgagcgcct gcggcgaatg gcgccgcaac acgacttagc gacggtcatc gcctgcgcct
     1081 tcgaccatcg cacgcggatc cttccgttca tctatgccga catgcggatg ccgccggcgg
     1141 gcgtgcgggc gatcggctcg gcgctggtcg atgccggctt ccccaaaacg cggatcgtcc
     1201 tgcagcagtg gaaccagaac ttccggccgt ctgagatgcg cctggacggg cgcattcccg
     1261 acctgttctg cgtgtcgagc atgcacctgc acggcagcga gtgcgaccgc ctgatccgcg
     1321 actgccagcg gattgatccc gcgcggcggc cgctgatcgt tgtgggcggg ccgcggatgg
     1381 tctatgagcc gtggctggcg ttcggcacag acgccgacaa tccctggggt gcggacgtcg
     1441 cggtcactgg cgaagaattc gtctttctca gtttgctcga ggtcctcctg tcgctgcggg
     1501 ctcagggtga accactgcgc tcggtattcc tgcgagcccg cgactccgga gcgctcgacg
     1561 aaataccggg actcgtctat gcccggcagt cccggcccga tggacccgcc gaagagctgg
     1621 tcgataccgg catccagcgg ctgctgggcg atctcgacga attgccgcat ccggtgctcg
     1681 gctaccagtt actcgaggcc cccagccgca agagtacgct ggcctccagc gcgctgccgg
     1741 cgaaacgcgt ccgccgctat tcagcgatct cgtcgattgt gcttacggta ggctgcaagt
     1801 tccgctgcgc gtattgcccg attcccgcct acaaccagcg gcagcaccgc acaaaatcgg
     1861 gcgagcggat cgccgacgaa atggggcgga tcaccagcac ctacggcatc agctttttct
     1921 tcggcaccga cgacaatttc ttcaatgaga ccaagcgcac gctcgaaatc gccgagacgc
     1981 tggcacgcaa ggcggccgag cgcaagcggc cgcactgcaa gattctgtac ggcacagagg
     2041 ccacggtcca cgatacgatc cgcatgcagg agcacctgcc gctgatccgc cgctcgggga
     2101 tggccgcggt ctggatgggc gtcgaggacc tcacgggtac gctcgtcaag aagggccaga
     2161 accagagcaa gaccatcgag gcgtttcagc tgctgcgggc cagcggcatc gccccggtgc
     2221 cgatgatgat gcaccacgac tcgcagcccc tcgtcactct caagagcaac tacgggctgc
     2281 tcaaccagct ccgcacgctc cgcaaagccg gagcgatgta cacgcaggtc ctgatgctca
     2341 cgccggcgcc tggctcgaag tggtacgaag acacttacac gtccggtctg gccttcgacg
     2401 ccgtgggcgg cgagccattg gctccgcaca tcgttgacgg caactacgtc gtggcttcca
     2461 agcacgcgcg gccctgggtc aaacagctca atctgctcat tgcctatctc tacttcttca
     2521 acccgctgcg gacgctgctc gcactcgcct tttcgctgtc gcggattccg ctggtcgatg
     2581 ccgacaaccg cccggctgcc gagattgccc gctactcgcc gctggcgcga atcgcccgca
     2641 cgctctatct aaa
//
LOCUS       DASRJB010000808         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_238681, whole genome shotgun sequence.
ACCESSION   DASRJB010000808 DASRJB010000000
VERSION     DASRJB010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238681"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFB80_21160"
     CDS             <1..613
                     /locus_tag="VFB80_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36358.1"
                     /translation="QQRQSRPLPRSRQLLGESLEPRCVLTALPADETAPSLSAYLAQP
                     HEPGPVPAESSIVLESDAQAVPAIDLSFVAEFAAGDSCGGFAGDPTATLAGDAPEGES
                     DSGPSSGTGGGGEENQPPNIATLSIVIDEGFLVIRGSVSDESPETCIVSLTGLITETT
                     TVDELGNFEFRLVSPQLFGALRFVARDDYGLQSPVWTEWLDPS"
     gene            706..1662
                     /locus_tag="VFB80_21165"
     CDS             706..1662
                     /locus_tag="VFB80_21165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN36359.1"
                     /translation="MQSTTRFSRRQFCAAGSAAVAAAATRLAPGRAAAAEVDLSKLPY
                     IDAHSHLWSPDVERWPLANNQTRADLKPPSFTPEELWKLAEPEGVGRAVLIQHSTYHA
                     FDNRYLIDCARRFPGRFAVVGMLDDTQPHPDVKLKELLPAGVRGLRITPRIRGEKWLE
                     GPGMEALWKTAAETGQAMCCLIDAKDLAAVNAMCARHPGTPVVIDHFARIGVDGEIKD
                     ADVKALCDLAKHKRVSVKISAYYALGKKQQPYDDLAPLIRRVLDAFGIERCMWASDSP
                     YQIVPPHTYAASIALVRDRLAGLSAGDKSWLLTKTAERVYFA"
     gene            complement(1705..2925)
                     /locus_tag="VFB80_21170"
     CDS             complement(1705..2925)
                     /locus_tag="VFB80_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36360.1"
                     /translation="MTAFLFRGARWRRLILESLERRQLLATLTVTGTPSDDTILASVS
                     GDLLTISVNGATFSVPSSAFDRINVFAGAGNDSVRIDASVPQATELHGQEGNDRLAAG
                     SGPSLMFGGAGDDGLTGGLAGDVLFGGLGHDSIAGGRGGDYLIGGSGNDKLSGESGHD
                     WLFGDATNSLPANAVDPVQYALDFMDVGTGNDALDGGEGNDYLFAGNGDDRAGGGAGT
                     DLIVGGVGDDGLGGGAGDDLILGDTLSTNPPGDADLVRAAERVSGDAALIVTSARRLS
                     PVPIPLPSFNDTIVGGEGEDTLLGQLGADVMNGNAGDDLLLGGDGRDHLAGDAGDDTL
                     LGGDGDDLLEGGDGADLLFGENGSDKLIGGAGLDLFDGGDGADKIDALDGQIDLIVGD
                     PLDGVDADPFDIFV"
BASE COUNT          519 a         1142 c         1047 g          542 t
ORIGIN      
        1 ccagcagcgg caatcacgcc ccttaccgcg ctcccgccaa ttattgggtg agtccctgga
       61 accccgctgc gtgttgacgg cgttgcccgc cgatgagacg gcgccttcgc tgagcgctta
      121 ccttgcgcaa cctcacgagc cgggtccagt tcccgctgaa agcagcatcg tcctggagtc
      181 ggacgcccaa gctgtaccgg cgattgacct gagcttcgtc gctgaatttg ccgcggggga
      241 ctcttgcggc ggcttcgcgg gcgatccgac tgcgacactc gctggggacg cgccggaagg
      301 tgaaagcgac agcggacctt catcgggaac cggcggcggc ggcgaagaaa atcagcctcc
      361 caacattgcc accctcagca tcgtcattga cgaaggcttt ctggtgattc gaggaagcgt
      421 cagcgatgag tcgcccgaaa cttgcatcgt ttcgctgacc ggtctgatca cggaaaccac
      481 gacggtggat gaactgggga attttgagtt tcgcctagtg tcgccccaac tgttcggcgc
      541 gctgcgcttc gtagcccgag acgactacgg cctccaatca cccgtctgga ccgagtggct
      601 ggaccccagt tgattgcggg cgcggtcccc ggccgagttg gcagtccgcc gcggctgcac
      661 gtatgatgtc cgccagacgt ttctcgtccg gcggagtcgc gtgccatgca atcgaccacg
      721 aggttctccc gccggcaatt ctgcgcggcg ggttctgccg cggttgctgc ggcggcaact
      781 cggctcgcgc ccggccgcgc ggccgctgca gaggtggatc tgagcaagct accctacatc
      841 gatgcgcact cgcatctctg gtcgccggac gtcgagcgtt ggccgctggc gaacaaccag
      901 acgcgggccg atctgaagcc gccgagcttc acccccgagg agctatggaa gctggcggag
      961 ccggagggcg tcggccgggc ggtgctgatc cagcacagca cttatcacgc cttcgacaac
     1021 cgctacctga tcgactgtgc ccggcggttc ccgggccggt tcgccgtcgt gggaatgctc
     1081 gacgacacgc agccgcaccc cgacgtgaag ctcaaggagc tgctgcctgc gggcgtgcgc
     1141 ggcctgcgga tcactccgcg aattcggggc gagaagtggc tcgaaggccc ggggatggag
     1201 gcgctctgga agacggccgc cgagaccggg caggcgatgt gctgcctgat cgacgccaag
     1261 gacctggccg cggtgaacgc gatgtgcgcc cgccacccgg gcacgccggt cgtcatcgat
     1321 cacttcgccc gcatcggcgt ggacggcgaa atcaaagacg ccgacgtgaa ggccttgtgc
     1381 gacctggcga aacacaagcg ggtcagcgtg aagatctcgg cctattacgc gctgggcaag
     1441 aagcagcagc cctacgacga ccttgcgccg ctgatccgcc gcgtgctgga cgcgttcggc
     1501 atcgagcggt gcatgtgggc cagcgactcg ccctatcaga tcgtcccgcc gcacacctac
     1561 gccgcctcga tcgccctcgt ccgcgaccgc ctcgccggcc tgtcggccgg cgataagagt
     1621 tggctgctca ccaagaccgc cgagcgggtc tatttcgcgt agggccagct gccggctggc
     1681 ccgtcctgtt tcgccggctg ttgctcacac gaagatgtcg aacggatcgg catcgacgcc
     1741 gtcgagcggg tcgccgacga tgaggtcgat ctggccgtcg agggcgtcga tcttgtcggc
     1801 gccgtcgccg ccgtcgaaga ggtccaggcc cgctccgccg atcagcttgt cgctgccgtt
     1861 ttcgccgaac agcaggtcgg ccccgtcgcc gccttccagc aggtcgtccc cgtcgccccc
     1921 cagcagcgtg tcgtcgcccg cgtcgccggc caggtggtcg cggccgtcgc cccccaagag
     1981 cagatcgtcg ccggcgttgc cgttcattac gtccgcgccg agctggccca gcagcgtgtc
     2041 ttcgccttcg ccgccgacaa tcgtgtcgtt aaagctcggc agcggaatcg gcacgggcga
     2101 gaggcggcgg gcgctggtca caatcagcgc cgcgtcgccg ctgaccctct cggccgcccg
     2161 cacgaggtcg gcgtcgccgg gcggattggt cgagagcgtg tcgcccagga tcaggtcgtc
     2221 gcccgcgccg ccccccaggc cgtcgtcgcc gacgccgccg acgatcaggt ccgtccccgc
     2281 cccaccgccc gcccggtcgt cgccgttgcc cgcgaagaga tagtcgttgc cttcgccccc
     2341 atccagcgca tcattgcccg tgccgacgtc catgaagtcc agcgcgtact gcaccggatc
     2401 gacggcgttg gccggcaggg agttggtcgc gtcaccgaac agccagtcgt ggcccgactc
     2461 gcccgagagc ttgtcgttcc cgctgccgcc gatcaggtag tcgccgccgc ggccaccggc
     2521 gatcgagtcg tggcccaggc cgccgaacag cacgtctccg gccagcccgc cggtcaggcc
     2581 gtcgtcgccc gccccgccga acatcaacga cggcccgctg cccgccgcca ggcggtcgtt
     2641 tccttcctgg ccatgcagct cggtcgcctg cggcacgctg gcgtcgatcc gcaccgagtc
     2701 gtttcccgcc ccggcgaaga cgttgatccg atcgaaggcg ctgctgggca ccgagaacgt
     2761 cgccccgttg acgctgatgg tcaagagatc cccgctcacc gacgccagga tcgtgtcgtc
     2821 gctcggcgtg cccgtcaccg tgagggtcgc cagcagctgc cgccgctcga gcgattccag
     2881 aatcagccgc cgccagcgcg caccgcgaaa cagaaacgct gtcatgtgcc acctcttccc
     2941 cgtaggaatg atgccgatca ggcgccgcag aattgcgaca atcgattagt ggcagcttgc
     3001 cacgctttcg tacgtggcac cgaaataatc tggcggcaca atacggtacc gagggcagcg
     3061 ccgctcgatt taacgcaaag gcgcgaagag gcaaaggcgc agagagttca aagagatgga
     3121 aacgccgtcg tactatttcc ttgcttcttt gcgccttctg cctcttcgcg cctttgcgtt
     3181 gaaggggagg cacgccacat cacagccccg cgccttttgc caagctacga gccggccgca
     3241 ggcgctagcg
//
LOCUS       DASRJB010000809         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3614317, whole genome shotgun sequence.
ACCESSION   DASRJB010000809 DASRJB010000000
VERSION     DASRJB010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614317"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFB80_21175"
     CDS             complement(<1..208)
                     /locus_tag="VFB80_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36361.1"
                     /translation="MTPAQRIEQLRDEIRGHDRRYYLEARPTISDRDYDRLMKELIDL
                     ERRHPELLAADSPTQRVGGDVQTEL"
     gene            complement(248..559)
                     /locus_tag="VFB80_21180"
     CDS             complement(248..559)
                     /locus_tag="VFB80_21180"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZN36362.1"
                     /translation="MNPLPHPIVRVTVCRQVGTYSLYLEFDDGATRTIDFEPILSGRV
                     YGPLRDPAQFAQVRLDPEIRTLVWPNDADFDPATLRYWDDYLPHLVARAEQLRRTPAS
                     V"
     gene            complement(556..825)
                     /locus_tag="VFB80_21185"
     CDS             complement(556..825)
                     /locus_tag="VFB80_21185"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HZN36363.1"
                     /translation="MPELSRFHGIIIRMFYEAGLPHHRPHFHAYYQDDVVSIAIDTME
                     VLAGSLPRRQQRLVEAWAELHQVELQAAWQAIVEEEPVTRIEPLR"
     gene            complement(832..1215)
                     /locus_tag="VFB80_21190"
     CDS             complement(832..1215)
                     /locus_tag="VFB80_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN36364.1"
                     /translation="MKVNHYESVPQQPVEMEGAGGCQVRWLLGERDGTPNFAMRQFEV
                     APGGFTPRHSHPYEHEVFVLEGQGVVFEGNRPHPLKAGDVVLVKPDEVHQFRNTGDAP
                     LKFLCLVPNSATGKKATVAPECGTE"
     gene            complement(1212..1511)
                     /locus_tag="VFB80_21195"
     CDS             complement(1212..1511)
                     /locus_tag="VFB80_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36365.1"
                     /translation="MPQSKPFNPFYVAALPVGVIFALTACSYMVMAYRGLDPHQADEG
                     GLVGLMDQHGLAILLVELALLAVLTFAAIGTDGYWTRRFEAAQALGAGNAAEREP"
     gene            complement(1535..2146)
                     /gene="coaE"
                     /locus_tag="VFB80_21200"
     CDS             complement(1535..2146)
                     /gene="coaE"
                     /locus_tag="VFB80_21200"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZN36366.1"
                     /translation="MLTLGLVGGIASGKSQVAQCLHDLGAVVLDADRAGHEVLREPDI
                     IAALRSRWGDKVLDAAGQISRRAVARIVFAAASEAEKQFLEQLTHPRIQMRLQQEMLR
                     AQSLSPPPRAIVLDAALLFEAGWNQLCEKILFVDAPRDVRLERAVARGWSAEQFAARE
                     AAQLPIEEKRSRADIVIRNVRTLESLREVVRLTWHSLVGQPDS"
     gene            complement(2188..>4135)
                     /gene="polA"
                     /locus_tag="VFB80_21205"
     CDS             complement(2188..>4135)
                     /gene="polA"
                     /locus_tag="VFB80_21205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZN36367.1"
                     /translation="IEKLAIKFGPCEPDEPAADDLPSGERGAESLAKPQAKTTADSPE
                     WKANYRTIATQEELEQLLATLSKQKRFVLDTETTTLHARAAQIVGYSFAWAPGEAVYV
                     PVRAPAGEPQLDPLAVQGALKPVLENPAIEKIGQNLKFDRIVLRAAGIELAGAAFDTM
                     VADYLLDPGERSHNMDDLALRHLGHQTITIDQLIGSGKKQKRMDEVPVPLITKYAAED
                     ADVPLRLAEVLAPRLREAGLAALFADLEMPLIEVLAEMEFQGIRVDVERLRELALQFE
                     SRIAQLKKEVFEIAGREFNIDSRIQLGQLLFDELKLPVTKRTKTGASMDSDVLEDLAP
                     LHPLPAKIIEYRHNAKLKSTYVDALPELICPATGRVHTSFKQDVAATGRLSSQDPNLQ
                     NIPIRTEIGRAIRSAFLPGEADWLLLTADYSQIELRVLAHFSGDATLRQAFAEDRDIH
                     AQVASEVYGVPLAEVTSAMRRSAKAVNFGVIYGQSAFGLARSLGIEQAEAARFIDRYF
                     AGYPGVDAFLLTTLRAARSQGYVSTISGRRRPISGVRDSRLLAAKRQRNLPERIAINT
                     VIQGSAADIIKQAMIGVLRRLRREKLRAKLLLQIHDELVFELPPEEEPALRQLVLEEM
                     AAAANLTVPLKVDVNVGRNWAECE"
BASE COUNT          672 a         1393 c         1324 g          746 t
ORIGIN      
        1 tcagctccgt ctgcacgtcg ccgccgaccc gctgcgtggg cgaatccgcc gcgagcagct
       61 ccgggtgtcg ccgctccagg tcgatcagct ccttcatcag ccggtcgtaa tcccggtcgc
      121 tgatcgtcgg ccgggcctcc aggtaatacc tgcggtcgtg tccgcggatt tcgtcgcgga
      181 gctgctcgat gcgctgggcg ggagtcatgc cgcagaagct agcggcagca atttcgaaac
      241 gcaacggcta cacgctggca ggcgtgcgcc ttagctgctc ggcgcgagct accaggtgcg
      301 gcagatagtc gtcccaataa cgcagagtgg ccggatcaaa gtcggcatcg tttggccaca
      361 cgagtgtcct gatctcggga tcgaggcgga cctgggcaaa ctgcgctgga tcgcggagcg
      421 gtccgtaaac gcggccgctg aggattggct caaaatcaat cgtgcgcgtt gcgccatcgt
      481 cgaattccag gtacaggctg tatgtaccga cttggcggca cacagtcacg cggacgatgg
      541 gatgcggcaa tggattcatc ggagcggctc aattcgagtc acgggctctt cctcaacaat
      601 agcttgccat gctgcctgca gctcaacctg gtgcagctcg gcccaggcct caacaagtcg
      661 ttgctgccga cgcggcaatg atcctgccaa tacttccatt gtatcaatgg ctatcgaaac
      721 tacatcgtct tgatagtacg cgtggaagtg aggccggtgg tgtggcagac cggcttcgta
      781 aaacatacga atgatgattc cgtgaaaccg cgacagctcc ggcatggtct cctactccgt
      841 cccgcattcc ggcgcgacgg tcgccttctt tcccgtcgcc gagttcggca cgaggcagag
      901 gaacttcagc ggcgcgtcgc ccgtgttgcg gaattggtgc acttcgtccg gcttgaccag
      961 caccacgtcg ccggctttca gcgggtgcgg ccgattgccc tcgaacacga ccccctggcc
     1021 ttccaggacg aagacctcgt gctcgtacgg gtggctgtgc cgcggcgtga agccgccggg
     1081 ggcgacctcg aactgccgca tcgcgaagtt cggcgtgccg tcgcgctcgc ccagcagcca
     1141 gcggacctgg cagccgccgg ccccttccat ctcgacgggc tgctgcggga cggattcgta
     1201 gtggttgact ttcatggctc gcgctcagca gcatttccag cacctaaggc ctgcgccgcc
     1261 tcaaaccgcc gcgtccagta gccgtcggtg ccgatcgcgg cgaaggtcag cacggccagc
     1321 agggccagct ccacgagcag gatcgccagc ccgtgctggt ccatcagccc gaccagcccg
     1381 ccctcgtcgg cctggtgcgg atcgaggccg cggtaggcca tgaccatata gctgcacgcg
     1441 gtcagcgcga agatcacgcc caccggcagc gcagcgacgt aaaacgggtt gaacggcttg
     1501 ctctgcggca tcttgtaatt atatcgcgcc gcccctacga atcgggctgg cccaccaggc
     1561 tatgccaggt cagccgcacg acctcgcgca ggctctcgag cgtccgcacg ttgcggatga
     1621 cgatgtcggc ccgcgagcgt ttttcctcga tcggcagctg ggcggcttcg cgggccgcaa
     1681 actgctcggc cgaccagccc cgggcgacag cccgttccag ccgcacgtcg cgcggggcat
     1741 cgacgaacaa gatcttctcg cagagctggt tccagccggc ctcgaataac agcgcagcat
     1801 ccaggacgat cgcccgcggc ggcggcgaaa gcgactgggc ccgcagcatc tcttgctgca
     1861 atcgcatctg gatgcggggg tgcgtcagtt gctccaaaaa ctgcttttcc gcttcgctag
     1921 ccgcggcaaa aacaatcctg gccacggccc gccggctgat ctggcccgcg gcgtcgagca
     1981 ccttgtcgcc ccagcggctc cgtagcgcgg caatgatgtc cggctcgcgc agcacctcgt
     2041 gcccggcacg gtcggcatcg agcacgaccg cgcccagatc gtgcaggcac tgcgcgacct
     2101 ggctcttgcc gctcgcgatg ccgccgacga ggccgagggt gagcatggcg gttccgagtt
     2161 ccgagttcca agttccgaga atccggctca ctcacactcc gcccaattgc gccccacgtt
     2221 cacatcgact ttgagcggca cggtgaggtt cgcggcggcg gccatctctt ccaggacgag
     2281 ctggcgcagg gcgggttcct cttcgggggg cagctcgaac accagctcgt cgtggatttg
     2341 cagcagcagc ttcgcgcgca gtttctcgcg ccgcaagcgg cgaaggacgc cgatcatcgc
     2401 ctgcttgatg atgtcggcgg cggagccctg gatcacggtg ttgatggcga ttcgctcggg
     2461 caagttgcgt tgccgcttgg cggcgagcag gcgcgagtcg cggacgcccg aaatgggccg
     2521 ccggcggccg gagatcgtgc tgacgtagcc ctgacttcga gccgcgcgca gcgttgtaag
     2581 caagaacgca tcgacgcccg gatagcccgc aaaatagcgg tcgatgaacc gcgccgcctc
     2641 ggcctgctcg atgcccagcg acctggccag gccgaacgcg ctttgcccgt agatcacgcc
     2701 gaaattcacc gccttcgcgc tccgccgcat cgcggacgtg acctccgcga gcggcactcc
     2761 atacacctcg ctcgccacct gggcatgaat gtcgcggtcc tcggcaaagg cctgccgcag
     2821 cgtggcgtcg ccggaaaaat gcgccagcac ccgcagctcg atctgcgaat agtcggccgt
     2881 gagcagcagc cagtccgcct ctccgggcag gaaggccgag cggatcgccc gcccgatttc
     2941 cgtgcggatc ggaatgttct gcaggttcgg gtcctgcgag ctgagccggc cggtcgcggc
     3001 cacgtcctgc ttgaaggaag tgtgcacgcg gcccgtcgcc gggcaaatca gctcgggcaa
     3061 ggcatcgacg tacgtgctct tcagcttggc gttgtggcgg tattcgatga tcttcgccgg
     3121 cagcgggtgc agcggcgcga ggtcttccag cacgtcggag tccatgctgg cgccggtctt
     3181 cgtgcgcttg gtgaccggca gcttcagctc gtcgaacagg agctggccca attggattcg
     3241 cgagtcgatg ttgaactcgc ggccggcgat ctcaaagacc tccttcttga gctgcgcgat
     3301 gcggctctcg aactggagag ccagctcgcg gagccgctcg acatccacgc gaatgccctg
     3361 gaattccatc tccgccagga cttcgatcag cggcatctcc aggtcggcga atagcgctgc
     3421 caatcccgcc tcgcgcagcc gcggcgcgag cacctcggcc agccgcagcg gcacgtcggc
     3481 gtcttcggcg gcgtatttcg taatcagcgg cacgggcact tcgtccatcc gcttctgctt
     3541 ttttccgctg ccgatgagct ggtcgatggt gatggtctga tggccgaggt gccgcagcgc
     3601 cagatcgtcc atgttgtggc tgcgctcgcc gggatcgagc aggtagtcgg ccaccatcgt
     3661 gtcgaacgcc gcgcccgcca gctcgatgcc cgccgcccgc agcacgatcc ggtcgaactt
     3721 caggttctgg ccgatcttct cgatcgccgg attctccagc accggcttta gcgcgccctg
     3781 taccgccagc ggatcgagct gcggctcgcc ggccggcgcg cgcacgggaa catagaccgc
     3841 ctcacccggc gcccaggcga acgaatagcc gacgatctgg gccgctcgcg catgcagcgt
     3901 ggtcgtttcc gtgtcgagga cgaaccgttt ttgcttcgac agcgtggcga ggagctgctc
     3961 cagctcttcc tgcgtcgcga tggtccggta gttcgccttc cactcgggcg agtcggcggt
     4021 cgtcttcgcc tggggtttgg caagcgattc agcgccgcgc tcgccggagg gcaagtcgtc
     4081 cgccgccggc tcatccggtt cgcagggccc gaacttgatc gccagcttct cgatc
//
LOCUS       DASRJB010000810         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1364310, whole genome shotgun sequence.
ACCESSION   DASRJB010000810 DASRJB010000000
VERSION     DASRJB010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364310"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..986
                     /locus_tag="VFB80_21210"
     CDS             204..986
                     /locus_tag="VFB80_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN36368.1"
                     /translation="MPRPLRVTIWNEYLHELEHDAVRKLYPEGIHAVLDAALSRLLAG
                     AVQTRIATLEQPEHGLTHAALAETDVLTWWGHAAHERVEDEIVERVHNRVLSGMGLVA
                     LHSAHASKIFRRLMGTTCMLRWREAAERERVWIVDPSHPITEGIADECFEIEHSEMYG
                     EHFDIPPPDELFAISWFEGGEVFRSGCTWRRGKGKVVYFSPGHETFPIYHDPNVQRII
                     ANAASWAAPAGGLYLGRGRNIQEPLSPIAAKHVVDESLHRPK"
     gene            1020..1844
                     /locus_tag="VFB80_21215"
     CDS             1020..1844
                     /locus_tag="VFB80_21215"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZN36369.1"
                     /translation="MFRTCTGIALSALFLAALPDPVAPDPNSPRPIDAVDSVFIEDLT
                     WMEVRDALKGGVDTAIVATGGVEQNGPYLVAGKHNYVLRGTTEAIARKLGKTLVAPIV
                     PFVPEGDISPPTSHMKYPSTISVREETYQALLTDIISSLKTHGFRRIILIGDSGGNQA
                     GMRDVAARLNAQWKGVPQVIFVPEYYDSAPVTAWVTGLGIKQEPEGLHDDFVMSAQLV
                     AVDPRTVRADQRIRAGNFKINGVSLAPIGQTAAWGRRIIDYRAELTIRAIQKRSQQ"
BASE COUNT          358 a          681 c          653 g          312 t
ORIGIN      
        1 acgcccgcga gaatctgctc gcgggtatca acaactgggc cgccgagcat ctgcgggcca
       61 agcgctatcg cgaggccgcc cgcctgatcc gccaggggct ggccatcgag ccggcgtatg
      121 ggccgctcgt ggccaatcag cggctgctgc cgtagctggc atgctgcggc tgatatgata
      181 gggctcctgc gaaggagccg cccatgccca ggccgctgcg ggtcacgatc tggaacgagt
      241 acctccacga gctcgagcac gacgccgtgc ggaagctcta tcccgagggc atccacgccg
      301 tgctcgatgc cgcgctgtcg cggctgctgg ctggtgcggt ccaaacgcgg atcgcgacgc
      361 tcgagcagcc cgagcacggc ctgacgcacg cggcgctggc cgaaaccgac gtgctgacct
      421 ggtggggcca cgcggcccac gagcgggtcg aggacgaaat cgtcgagcgc gtccacaatc
      481 gcgtgctgag cggcatgggc ctggtcgcgt tgcactcggc ccacgcctcg aaaatcttcc
      541 ggcggctgat ggggacgacc tgtatgctcc gctggcgcga ggcggccgaa cgcgagcggg
      601 tctggatcgt cgatccgtcg catccgatca ccgagggaat cgccgacgag tgttttgaga
      661 tcgagcacag cgagatgtac ggcgagcatt tcgacatccc gccgcccgac gaattgttcg
      721 ctatcagctg gttcgaaggg ggcgaggttt ttcgcagcgg ctgcacgtgg cgtcgcggca
      781 agggcaaggt cgtctatttc agcccggggc acgagacctt tcccatctac cacgatccga
      841 acgtgcagcg gatcatcgcc aacgccgcga gctgggccgc cccggccggc ggcctgtacc
      901 tgggccgcgg ccgcaatatt caagagccgc tgagcccgat cgcggcgaag cacgtcgtcg
      961 atgagagcct gcaccggccg aagtagtttc ctgaccaact ttccgcatcg aggtgaacca
     1021 tgttccgaac ctgcaccggt atcgcactca gtgctctgtt tctggccgcc ctgcccgatc
     1081 cggtcgcgcc ggacccgaat tcgccgcggc cgatcgacgc ggtggactcg gtgttcatcg
     1141 aggacctgac ctggatggag gtccgcgacg cactcaaagg cggcgtcgat acggcgatcg
     1201 tggcgaccgg cggcgtcgag cagaacggac cgtacctggt cgcgggcaag cacaactacg
     1261 tgctccgcgg cacgaccgag gcgatcgccc gcaagctggg caagacgctc gtcgcgccga
     1321 tcgtcccctt cgtgcccgag ggagacatca gcccgccgac ttcgcatatg aaatatccca
     1381 gcacgatcag cgtccgcgaa gaaacctacc aggccctgct gaccgacatc atctccagcc
     1441 tcaagacgca cggcttccgg cggatcatcc tgatcggcga cagcggcggc aaccaggctg
     1501 gcatgcgcga cgtggccgcc cgactgaatg cccagtggaa gggcgtgccg caggtgatct
     1561 tcgtgcccga gtattacgac agcgcgcccg tcaccgcctg ggtcactggg ctgggaatca
     1621 agcaggagcc ggaagggctg cacgacgact tcgtgatgag cgctcagctc gtcgcggtgg
     1681 atccgcggac cgtgcgagcc gaccagcgga tccgcgccgg caacttcaag atcaacggcg
     1741 tttcgctcgc ccccattggc cagaccgccg cctggggccg ccggatcatc gactaccgcg
     1801 ccgagctgac catccgagcc atccagaaac gctcgcagca ataacgccgc agcagccggc
     1861 ctactggccg aacctgcggg ttgctatctt tctcgtaggt cccttctgcc ggaagggacc
     1921 ttgagggaag cgcagcgacc aacaccgcgg cttcgggcaa gcgttcgccc caagtcccgc
     1981 ccggcaggcg ggacctactt gcaa
//
LOCUS       DASRJB010000811         9979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1640898, whole genome shotgun sequence.
ACCESSION   DASRJB010000811 DASRJB010000000
VERSION     DASRJB010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640898"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFB80_21220"
     CDS             <1..731
                     /locus_tag="VFB80_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN36370.1"
                     /translation="TSRQHVAPVMRAEERCEDFVLDGYTPWVTGAEYAQHIVLGATLA
                     DRRQILVALPTDLAGVRIPPAPELVGLSASRTGPVECDRVEIERRWLLAGPVPEVMKQ
                     GLGAKTGGLQTSALAIGLAGAAIEFVQRQSDARSDLIEPAEALAAEREELMLAMLTLA
                     GETGMPQRAFPTCVADGNELRQRANSLALRASQAALAAAKGAGYVAGHPAGRWCREAL
                     FFLVWSCPAPVLQGNLCEFAGLAD"
     gene            812..1480
                     /gene="cysC"
                     /locus_tag="VFB80_21225"
     CDS             812..1480
                     /gene="cysC"
                     /locus_tag="VFB80_21225"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZN36371.1"
                     /translation="MTTDKPHIVWHPPSVSREDRERASGQQGCVVWFTGLSGSGKSTV
                     ANAVDRLLFEQGLRSFVLDGDNVRHGLNAPPALLADYGDEFARRFGLSFTAQDRQENI
                     RRIGAVADLFAAAGLITLTAFVSPYRADRDAVRRQVESSRTGSFVEIFVDAPLEICEA
                     RDPKGLYKKARAGELKNFTGIDDPYEPPQSPELRLDAAANSPAALAAEVLAFLRQSGK
                     ISRI"
     gene            1637..1897
                     /locus_tag="VFB80_21230"
     CDS             1637..1897
                     /locus_tag="VFB80_21230"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN36372.1"
                     /translation="MAQTLLANVASQAKAALSRSRIFDLRRLSVEQDGDYVVLRGRVD
                     SFYHKQLAQELVRAAVEGIEVLNAISVVYHRDRRDDFVDWRW"
     gene            complement(1921..2979)
                     /gene="lpxK"
                     /locus_tag="VFB80_21235"
     CDS             complement(1921..2979)
                     /gene="lpxK"
                     /locus_tag="VFB80_21235"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZN36373.1"
                     /translation="MLSDAQFRELVSGRWKGPLAGGLRAGLALLEPLYAATIRRRNRQ
                     FDTGRRQPHRVAAPVISVGNLTVGGTGKSPLVAWLADWLSRRGIAATIISRGYGARGG
                     RPNDEALELAARLPGVPHLQNPDRLAAAEEALRANPRQVLILDDAFQHRRIARDLDIV
                     LLDALEPFGYERLLPRGLLREAVENLARAQVVALSRSDAVNPARRTEIESRVKCVAPQ
                     AAWLELEHRPGGLVSASGRRMELAALSGLSVAAFCGIGNPAGFRHTLAAAGLSVAEML
                     ELPDHCPYDGRTMQRLTLWVSRLPQATAVLCTRKDLVKIPQDEIAGRPLWALAIEMAI
                     TRGEALLANRLESLLKSV"
     gene            complement(3004..3927)
                     /locus_tag="VFB80_21240"
     CDS             complement(3004..3927)
                     /locus_tag="VFB80_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN36374.1"
                     /translation="MQLGFVSAILPDLSLSEVARFAAAAGFDCVELMCWPVSKAERRY
                     AGVTHVDVTDFTSAKADEVRRIMADAKVAISGLGYYPNPLTTNREESELAVSHLRKVI
                     QGAALLGLKTVNTFIGRDWTRSVDDNWPRLLEVWKPLVKFAEGNGVRIGIENCPMLFS
                     KDEWPGGKNIATSPKLWRRLFGDVGSASLGLNYDPSHMIWQQMDYLRPLRDFKDRLFH
                     VHAKDVRIDRERLNEVGILAHPNEYHTPKLPGMGDVNWGQFFSVLTDTGYAGPVCVEV
                     EDRAYEHSLEARKASLVQSCTYLRNFVPASR"
     gene            4122..5753
                     /locus_tag="VFB80_21245"
     CDS             4122..5753
                     /locus_tag="VFB80_21245"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HZN36375.1"
                     /translation="MSAAILAAMLLAAEPRAADAVLVAPREFLPALKPLIEHRQKQGH
                     GFVYVPTASTPEGIRSGIRDAAKAGGLKFALLVGDAEPAARVDRAVAARCVPAHLEKA
                     VVNVKWGSEPQIASDNWYADLNDDQLPDLAIGRIPADTPAEVARIVAKILAYEQSADF
                     GPWRERVNLIAGVGGFSPILDTVLETATSKLLTSGIPAGYETRMTYGSWRSPYCPDPR
                     LFHLTAVERHNEGCLFWIYIGHGSPTALDQVSVPGERFHIFDVDDCQKLKSAGGAPIA
                     LMLACYTAAFDQPQDCLAEELLKADGGPVAVYGGSRVTMPYAMGVMGTAMMDEYFKTR
                     PPTLGEVVLAAKRKMVQPIDETNAAANLNRVLLDGVAAVISPSREMLDAERREHLHLF
                     HLIGDPMLRLTYPDEIGLSAPRDASAGAKLRISGRTKMAGPALLELVCRRDQHKVAPP
                     ARERFDPTDKALAAFQGVYEQALDRCWARWALDLPAGEFATDITLPAECRGPCHFRLL
                     VANGSSYGLGATSLYIRQPEAPTQIAQPAANTGAR"
     gene            complement(5754..6524)
                     /locus_tag="VFB80_21250"
     CDS             complement(5754..6524)
                     /locus_tag="VFB80_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36376.1"
                     /translation="MTTCAVLLDQAGELPPPDLLADVIETQAGFPRPAAVRLATRARW
                     FIWENAPAESAQAVAQGLSAAGFATRAVPQGAVVPPTNPRRVHVLNLEGDALGVQLKY
                     TGPLTWIPWDEVLVLSAGAIKTETRTTEVRETVVARGIVVVDSRVQVDLERAILAEIY
                     ATAPSLGELLCLRLNSREVNYAQTIGGSINESWREKFSLLLARLGLRATSAAVSPQTE
                     ALLAAGLMPDNAALNPYFSSEDEFALYNRWLATRKRLG"
     gene            complement(6563..>9979)
                     /locus_tag="VFB80_21255"
     CDS             complement(6563..>9979)
                     /locus_tag="VFB80_21255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36377.1"
                     /translation="QRVAAHLDICPACERAAAELSDDQEARELAAAAGGQPAEPKPEP
                     QVEDLCKRLVALGLYESALDEAEEDTRPGLDPEPSQTSVLSKGRAPQTVVRPTVQNLP
                     AVALPATSELTPSCDPAIDGRRLKRLGRYEIVRLLGAGTFGIVYLAQDCKLNRPVALK
                     IARATVLADDHLRLRFIREAETLAKLRHPHIVPVYEADEVEGLCYLAIAFVDGPSLDE
                     WLRQQEGPLDSRLAVKLLLPLVDAVEHAHAHGVLHRDIKPGNIILERAEENTDGLTVV
                     SRLTDFGLAKVVEEKSSSTISGIVLGTAEYMAPEQAAGHVERIGRPTDIYGLGAVLYH
                     MLTGRPPICGNTTIDTLRRLLVDEPAEVRTLNRNVPADLDAIVLRCLNKQVQDRYATA
                     AEMAADLRRFLEGHPTIARPLSLRQRVGRWLHRNPTLTSAIALATIAVCLTVGWYRAS
                     QMLGASTRAIDIASSKSERMQRQLAQTREELEERARQIALSQYHDAITAAERALAEGD
                     VVQALTALQGQIPPPLSPPTSDLRGLEWHYLYALATQTPLAEAFLGSEIYQIRLSPDR
                     REVAAACRDSVLRLYDSRKLTELKSIATDQIEINGVAYSPDGKVIATAGDDGTIKIYD
                     LGTRELLVRILAHSCKSYGVTFYDGSSKLASCGEESRVRLWDAASGDGLGYLDGHSDL
                     VEALALSPDGRYLATASSDRTCRVWDLREERLPQILSGHTARLTSVWFSPDGRQLATG
                     CLDKQVALWDLATGRRTTESHLDQVQSVCFSSSGEWLFAGDRSGAVRKYRIGPGPYQK
                     LVRDPAEDTWQAHDTRVWATIPGPEEGTLLTCGARGYLKLWGGVRRQVRRVCPETLDD
                     NAVDLAFSGEQLFVLHGTGGIEMLDAATLATRGWLTARLTDWNCLEVMGAEEVAAGDT
                     HGNVAIWNHRSGVLRRIISHPDENFTVLGLVYSQPARLLAVLPATHHLRLYKSDTGEP
                     AGILPTNNHTAAAISPDGRQLAVDTSDNILLYDPIGKGYLDSLRGHRQTIYGLAYSPS
                     GRLLASASADRTARLWGAQGGEPRVLGGHRAGLADVAFTPDEKTLVTIDDAGVLKLTH
                     VATARTLLDIPTDTKSLRALAVAPDSRRLAVLRQDYSILLIGAPPEKPGE"
BASE COUNT         1605 a         3336 c         3391 g         1647 t
ORIGIN      
        1 cgaccagccg gcagcacgtc gcgcccgtca tgcgggcgga ggagcgctgc gaggattttg
       61 tcctggatgg ctatacgccc tgggtgaccg gcgcggagta tgcgcagcac attgtgctag
      121 gcgcgacgct ggccgatcgg cggcaaatcc tggtcgcgct gccgacagac ctggccggcg
      181 tgcggattcc gcccgcgccg gagctggtcg gcctgtcggc cagccgcacg gggccggtgg
      241 agtgcgaccg cgtggagatc gagcggcgat ggctgctggc cggacccgtt cccgaggtga
      301 tgaagcaggg tctgggtgcg aagacgggcg ggttgcaaac atcggcgctg gccatcggcc
      361 tggccggcgc ggcgatcgaa tttgtgcagc ggcagtcaga tgcccgcagc gacctgatcg
      421 agccggccga ggcgctggcg gcggagcgcg aagaattgat gctggcgatg ttgacgctcg
      481 ccggcgaaac gggaatgccc cagagggcgt tccctacatg tgttgcggat gggaacgagc
      541 tgcggcagcg tgccaacagc ctcgcgctgc gggccagcca ggcggcgctg gcagccgcta
      601 agggcgcggg ctacgtcgcc ggtcatcccg ccggccgctg gtgccgcgag gccctgtttt
      661 ttctggtttg gagttgcccg gccccggtgc tgcaaggcaa tctgtgtgaa ttcgcggggc
      721 ttgcggatta gtccgttgtc agttgccagt tgggcgcaaa caaatgacac cggcaactcc
      781 cagctgacct ctgcctactg accactgacc aatgacaact gataaaccgc acatcgtctg
      841 gcacccgccg agcgtcagcc gcgaggaccg cgagcgggcc agcggtcagc agggctgcgt
      901 agtctggttc acggggcttt cgggcagcgg taagagcacc gtggccaacg cggtcgatcg
      961 gttgctgttc gagcaggggc tgcgaagctt tgtgctcgat ggcgacaacg tgcggcacgg
     1021 gctcaacgcg ccgccggcgc tgcttgccga ctacggcgac gagttcgccc gccgctttgg
     1081 actatcgttt accgcacagg atcggcagga gaacattcgc cgcatcggcg cggtagcgga
     1141 cttgtttgcc gcggctggcc tgatcacact caccgcgttc gtcagccctt accgcgccga
     1201 tcgcgatgcc gtccgccggc aggtcgagag cagccggacg ggcagcttcg tcgaaatctt
     1261 cgtcgatgct cctctggaaa tttgcgaagc tcgcgatccc aaaggcctat acaagaaggc
     1321 tcgcgcgggg gagctcaaga attttacggg catcgatgac ccctatgagc cgccgcaaag
     1381 cccggaattg cggctcgatg cggcagcgaa ttcgcccgca gccctcgccg ccgaagtgct
     1441 agcttttctg cgccaaagcg ggaagatttc aaggatttaa cggaacatcg cgttgacagg
     1501 cagactaaca cccttacatt gtaggatgcc gggtgggggt cgcgtcgcgg gcctcccggc
     1561 tgatacgacc gacatggaat tcgccgtcgc gccgcctcat ctttcggcgc gttggccacg
     1621 gacggaggct ggtacagtgg cccaaaccct cctagcgaac gttgcctctc aagccaaggc
     1681 tgccctttcg cgcagccgca tcttcgacct ccgccggctg tcggttgagc aggacggaga
     1741 ctacgtcgtc ttgcgtggcc gggtggattc gttctatcac aagcagctgg cccaggaatt
     1801 ggtgcgggcg gccgtcgaag ggatcgaggt tttgaacgcg atcagcgtcg tctatcaccg
     1861 cgatcgccgc gacgacttcg tcgattggcg ctggtaggtg gggcgggcgt ctggcccgcc
     1921 ctagaccgat ttcagcagcg actcgaggcg gtttgctagc agcgcctcgc cgcgcgtaat
     1981 cgccatttca atcgccagtg cccagagtgg gcggccggcg atttcgtcct gaggaatctt
     2041 caccaggtcc ttgcgcgtgc agagcacggc cgtggcctgt ggcaggcgcg aaacccacag
     2101 ggtcagccgc tgcatcgtcc gcccgtcgta gggacagtgg tcgggcagct ccagcatctc
     2161 cgcgaccgac aggcccgcgg cggcgagcgt gtgccgaaag ccggccgggt tgccgatgcc
     2221 gcagaaggcc gcgacgctca ggccgctcag cgccgccagc tccattcgcc ggccgctggc
     2281 cgagacgagg ccgccgggcc gatgctccaa ttccagccac gccgcctgcg gtgcgacgca
     2341 cttgacgcgc gactcaatct cagtccgccg tgcaggattt acggcatcgc tgcgcgacag
     2401 cgccacgacc tgcgcccgcg ccaggttctc gacggcctcg cgcaacaggc ctcgcggcag
     2461 gaggcgctcg tagccaaaag gctccaatgc gtcgagcagg acgatgtcca ggtcgcgggc
     2521 gattcggcga tgctgaaagg cgtcgtcgag gatcaggact tgccgcggat tggctcgcag
     2581 ggcttcttcc gccgcggcca ggcgatcggg attctgcaaa tgcggcacgc cgggaagccg
     2641 cgccgccaat tccagtgcct cgtcgttcgg ccggccgccc cgcgcgccgt acccgcggct
     2701 gatgatggtg gcagcaatgc cgcgccgcga cagccagtcg gccagccagg ccaccagcgg
     2761 agatttgccg gtgccgccga cggtgaggtt gccgacgctg atcacgggcg cggccactcg
     2821 gtgcggctgg cggcgacccg tgtcgaactg ccgattgcgg cgacgaatcg tggcggcata
     2881 aagcggttcc agcagtgcca ggccggcccg caggccgccc gcgagcggtc ccttccagcg
     2941 gccgctgacc agctcgcgaa actgcgcatc cgaaagcatg acatccctgt ccggcgagct
     3001 gccttaccgc gaagcgggca cgaagtttcg caaatacgtg cagctctgca ccaagctggc
     3061 cttccgcgcc tcgagcgaat gttcgtacgc ccggtcttcc acttcgacgc agacggggcc
     3121 cgcgtagcct gtgtcggtga gcaccgagaa gaactgcccc cagttcacgt cgcccatgcc
     3181 gggcagtttc ggcgtgtggt attcgttcgg atgggccagg atgccgacct cgttcaggcg
     3241 ctcgcggtcg atccgcacgt ccttggcgtg gacgtggaac aggcggtcct tgaaatcccg
     3301 gagcggccgc aggtaatcca tttgctgcca gatcatgtgc gacggatcgt agttgaggcc
     3361 caggctggcc gagccgacgt cgccaaacag ccgccgccag agcttcgggc tggtggcaat
     3421 attcttgcct cccggccatt cgtccttgct gaacagcatc gggcagttct cgatcccgat
     3481 ccgcacgcca tttccttccg caaatttcac cagcggcttc cagacctcca acagccgcgg
     3541 ccaattgtca tcgacggagc gcgtccagtc gcggccgatg aacgtgttaa cggtcttgag
     3601 gcccaagagc gccgcgccct ggatgacctt ccgcaagtgg ctgactgcca gctcgctctc
     3661 ctcgcggttg gtcgtaagcg ggttgggata gtagcccagg ccgctgatcg ccactttggc
     3721 gtcggccatg atccgccgca cctcgtccgc cttggccgac gtgaagtcgg tgacatcgac
     3781 gtgcgtcacg ccggcatacc gccgctcggc cttgctcacg ggccagcaca tcagctccac
     3841 acagtcgaaa cccgccgcgg cggcgaagcg ggcgacctcg gagagcgaca gatcgggcag
     3901 aatcgcgctg acgaagccga gttgcatggg agggttcagg gtttcggggt ttcagggaag
     3961 caggtgcgat ttcaatgtac cgggcgttgc tgctccagga caccgcggat tgcccagttc
     4021 gccttgccgt cgtcaaagcc cgcaagtaca ctccgacgcc ctgctactcg tataatcgat
     4081 gactcgcacg ccgcaatccg gcgggatgaa gggatcgctg catgtccgct gcaattctgg
     4141 ccgcgatgtt gctggccgct gaaccgcgcg cggcagacgc ggtgctcgtt gcgccgcggg
     4201 aattcctgcc ggcgctaaag ccgctgatcg agcacaggca gaaacaggga cacggattcg
     4261 tttatgtacc gacggcgagc acgccagaag gaattcgcag cggcattcgc gatgcggcga
     4321 aggcgggcgg gctgaagttc gcgctgctgg tcggcgacgc cgagcccgcg gcgcgggtcg
     4381 atcgagcggt cgccgcccgc tgcgtgccgg cgcacctgga aaaggccgtc gtcaacgtga
     4441 agtggggcag cgagccgcaa atcgccagcg acaactggta cgccgacctg aacgacgacc
     4501 agcttcccga tctggcgatt ggccgcattc cagccgatac gccagccgaa gtcgcccgga
     4561 tcgtcgcgaa gatcctggcc tatgaacaaa gcgccgattt cggcccctgg cgcgagcgcg
     4621 tcaacctgat cgccggcgtc ggcggcttca gcccgatcct cgacacggtc ctggagactg
     4681 cgaccagcaa gctgctgact agcggcattc ccgccggcta cgaaacgcgg atgacgtacg
     4741 gcagctggcg cagcccctac tgccccgacc cgcggctgtt ccacttgacg gcggtggagc
     4801 ggcacaacga ggggtgcctg ttctggattt acatcggcca cggcagcccg acggccctcg
     4861 accaggtgag cgtgcccggc gagcggttcc acatcttcga cgtcgatgac tgccagaagc
     4921 tgaagtctgc gggcggtgcg ccgatcgccc tgatgctggc ctgctacacg gcggccttcg
     4981 atcagccgca ggactgcctg gccgaggaat tgctcaaggc cgacggcggt cccgtggcgg
     5041 tctatggcgg ctcgcgggtg acaatgcctt acgcgatggg cgtgatgggc acggcgatga
     5101 tggacgagta cttcaagacc cgcccgccga cgctgggcga ggtcgtcctg gccgccaagc
     5161 ggaagatggt ccagccgatc gacgagacca acgccgcggc gaacctgaac cgggtgctgc
     5221 tcgacggcgt ggccgctgtg atcagcccca gccgcgagat gctcgacgcc gagcgccgcg
     5281 agcacctgca cctgttccac ctgatcggcg atccgatgtt gcggctgacc tatcccgacg
     5341 agatcggcct gtcggcgccg cgcgacgctt cggcgggggc caagctgcgg atcagcggcc
     5401 gcacgaagat ggccgggccg gccctcctgg aactggtctg ccgccgcgac cagcacaagg
     5461 tcgcgccgcc ggcgcgcgag cggttcgatc cgaccgacaa ggctctggcc gctttccagg
     5521 gcgtgtatga gcaggccctc gaccgctgct gggcccgctg ggcgctcgat ttgcccgcgg
     5581 gcgagttcgc caccgacatc acgctgcccg ccgaatgccg cggcccctgc cacttccgcc
     5641 tgctcgtcgc caatggcagc tcctatggcc tcggcgcgac gagcctctac atccggcaac
     5701 cggaagctcc gacgcaaatc gcgcaaccgg ctgcaaacac gggcgcgaga tgactacccc
     5761 agccgcttcc gcgtagccag ccagcggttg tagagggcga actcgtcttc gctcgagaaa
     5821 tagggattga gcgcggcgtt gtcgggcatc aggcccgcgg cgaggagggc ttcggtctgc
     5881 gggctgacgg ccgccgaagt cgcgcggagg ccgaggcgag ccaagagcag cgagaacttt
     5941 tcccgccagc tctcgttgat cgagccgccg atcgtctgcg cgtagttcac ctcgcggctg
     6001 ttcagccgca ggcagagcag ctcccccaag ctgggcgcgg tggcgtaaat ctctgccagg
     6061 atggcccgct ccagatcgac ttggacgcgg gaatcgacca cgacgatgcc gcgggcgacg
     6121 accgtttcgc gaacctcggt cgttcgcgtc tcggttttga tcgcgccggc cgagagcacg
     6181 agcacttcgt cccagggaat ccaggtgagc ggtccggtgt atttgagctg tacgcccagc
     6241 gcgtcccctt ccaggttgag cacgtggacg cggcgcgggt tggtcggcgg cacgaccgcg
     6301 ccctgcggca cggcgcgggt ggcaaagccg gcggccgaca gcccctgagc gacggcctgc
     6361 gccgattcgg ccggcgcgtt ttcccaaata aaccagcggg cccgcgtcgc cagccgcacc
     6421 gccgccgggc gcggaaagcc cgcctgcgtc tcgatgacgt cggccagcaa gtcgggcggc
     6481 ggcaattcgc ccgcctggtc gagcagaacg gcgcaggttg tcatggcccc tcagccctta
     6541 agcccgttcc ccttgcctcg ccttactcgc ccggtttctc cggcggtgcg ccgatcagca
     6601 agatgctgta gtcctgcctg aggacagcca ggcggcggct gtcgggcgca acggccaagg
     6661 cccgcaggga cttggtgtcg gttggaatgt cgagcagggt gcgggccgtg gcgacgtgcg
     6721 tgagcttgag aacgccggca tcgtcgatgg tgacgagcgt cttttcatcc ggcgtaaagg
     6781 ccacatcggc caggccggcc cggtgcccgc ccagaactcg cggctcgccg ccctgggcgc
     6841 cccacaagcg ggccgtgcga tcggcgctgg ccgacgccag caggcggccg ctggggctgt
     6901 aggccaaacc gtagatggtc tggcggtggc cgcggagcga atccagatac cctttcccga
     6961 tcgggtcgta gagcaggata ttgtcgctcg tatcgaccgc cagctgccga ccgtcgggcg
     7021 aaatcgcggc tgccgtgtga ttgttcgtgg gcagaatccc ggcaggctcg ccggtgtccg
     7081 atttgtacag ccgcaagtgg tgcgtcgcgg gcaggacggc gagcaggcgg gcgggttgcg
     7141 aatagaccag tcccaagacg gtgaagttct catcggggtg gctgatgatg cgccgcaaaa
     7201 caccgctgcg gtgattccag atggcgacgt tgccgtgcgt gtcgccggcg gcgacctctt
     7261 ccgcgcccat gacttccagg caattccaat ccgtcagccg cgcggtcagc cagccgcggg
     7321 tggccagcgt cgcggcgtcg agcatctcga ttccgccggt cccgtgcagg acgaagagct
     7381 gctcgccgct gaaggccaga tcgaccgcgt tatcgtcgag cgtctcgggg cagacgcggc
     7441 ggacctggcg gcggacgccg ccccacaact tcagataccc gcgagcgccg caggtcagca
     7501 gcgtgccctc ttcgggcccg ggaatcgtgg cccagacgcg cgtgtcgtgg gcctgccagg
     7561 tgtcctccgc cgggtcgcgg acgagcttct ggtacggccc cggtccgatg cggtacttgc
     7621 ggaccgcgcc gctgcggtcg ccggcgaaca gccactcgcc cgagctggag aaacacacgc
     7681 tctgaacctg atccagatgc gactcggtcg tgcggcggcc ggtcgccagg tcccacagcg
     7741 cgacctgctt gtcgaggcag ccggtcgcca gttgccggcc gtcgggcgag aaccagacgc
     7801 tcgtcagcct ggccgtatga ccgctgagga tttgcggcag gcgctcttcc cggaggtccc
     7861 agacccggca ggtgcggtcg ctggaagccg tggccaaata gcgcccgtcg ggcgacagtg
     7921 ccagcgcctc gacaaggtcg gaatggccgt cgagatatcc caggccgtcg ccgctggcgg
     7981 cgtcccacag gcggacccgc gactcctcgc cgcaactggc cagcttgctg gagccgtcgt
     8041 aaaacgtcac gccatagctc ttgcaggaat gcgccagaat ccgcaccagc agctcgcggg
     8101 tgcccaggtc gtagattttg atggtgccgt cgtcgccggc cgtggcaatt accttcccgt
     8161 cgggcgaata ggccacgccg ttgatctcga tctgatcggt cgcgatcgac ttcagctcgg
     8221 tcagcttgcg ggagtcgtac aggcgcagca cgctgtcccg gcaggcggcg gccacctcgc
     8281 ggcggtccgg cgacaggcga atctggtaaa tctccgagcc gaggaaagcc tccgccaggg
     8341 gcgtttgcgt ggccagagcg tacagatagt gccactccag gccgcggaga tcgctggtgg
     8401 gcggacttaa gggcggggga atctgcccct gcagcgccgt cagggcctgc accacgtcgc
     8461 cctcggccag cgcccgctcg gcggcggtga tggcgtcgtg atattggctg agggcgattt
     8521 gccgcgccct ttcctccaac tcttctctcg tctgagccag ctgccgctgc atccgctcgg
     8581 atttggacga ggcaatgtcg atggcccggg tgctggcccc cagcatctgg ctggcccggt
     8641 accagccgac agtcaggcag accgcgatgg tggccagagc gatggcgctg gtcagggtcg
     8701 ggttgcggtg cagccagcgt cccacgcgct gccgcagcga gagcgggcgg gcgattgtgg
     8761 gatgcccctc caaaaaccgc cgcaggtcgg cggccatctc ggccgccgtc gcatagcgat
     8821 cctgcacctg cttgttcaag cagcggagga caattgcgtc caggtctgcc ggcacgttgc
     8881 ggttaagcgt gcggacttcc gccggctcgt cgaccagcag ccggcggagt gtatcgatgg
     8941 tcgtattgcc gcaaatcggc gggcggcccg tgagcatgtg atagagcact gcccccagcc
     9001 catagatgtc ggtcggccgg ccgatccgct cgacgtggcc cgccgcctgc tccggggcca
     9061 tgtactccgc cgtccccagc acgatgccgc tgatcgtcga gctgctcttc tcctccacga
     9121 cctttgccag gccgaagtcg gtcagccgcg aaacgaccgt caatccgtcc gtgttctctt
     9181 cggcccgctc caggatgatg ttgcccggct tgatgtcgcg gtgcagcacg ccgtgggcgt
     9241 gggcgtgctc gacggcgtcc accagcggca agagcagctt cacggccagc cgcgagtcca
     9301 gcggaccctc ctgctggcgc agccactcgt cgagcgaagg gccatcgaca aacgcgatcg
     9361 ccaggtaaca cagcccctcg acctcgtccg cctcgtagac gggcacgatg tgcggatgcc
     9421 gcagcttggc cagcgtttcg gcctctcgga tgaaccgcag ccgcagatgg tcgtcggcca
     9481 ggacggtggc ccgggcgatc ttgagggcca cggggcggtt gagcttgcaa tcctgggcca
     9541 gatagacgat gccgaacgtg cccgcgccca ggagccggac gatctcgtag cggcccaggc
     9601 gcttgagccg ccggccgtcg atcgccggat cgcagctggg cgttagctcg cttgtggcgg
     9661 gcagcgccac tgccggcaag ttctgcacgg tcggccggac gacggtttgc ggcgcgcggc
     9721 ccttggacag caccgacgtt tgcgacggct ccggatcgag accgggcctc gtgtcttctt
     9781 cggcttcatc gagggccgat tcatacagcc ccagcgccac caggcgcttg cacaagtcct
     9841 caacctgggg ctccggcttc ggctcggcag gctggccccc ggcagcggcc gccagttcgc
     9901 gagcctcttg atcgtccgac agctccgccg ccgcccgctc gcaggccggg cagatatcca
     9961 gatgggccgc cacgcgctg
//
LOCUS       DASRJB010000812        12268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1091557, whole genome shotgun sequence.
ACCESSION   DASRJB010000812 DASRJB010000000
VERSION     DASRJB010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091557"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFB80_21260"
     CDS             <1..1104
                     /locus_tag="VFB80_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN36378.1"
                     /translation="SGLAAFTVNADGASQLYLLTATVARDSVAGGPPRSGGLPFRPQR
                     RELKLPIGLVGSLEFSPDGKQLGFTLAAPDAPAEAYSLEIDSGRLTRWTYSEVGGLDP
                     QTFARAERIRFPSFDKREIPGWYFKPKNASPQRKAPVLIQIHGGPESQAQPLFSGTTQ
                     FYLNELGVAVLLPNVRGSAGYGKSYLKLDNAQLREDSVKDIGGLLDWIARQPELDASR
                     VAVSGGSYGGFMVLSSLTHFGDRIKAGIDVVGICNFNTFLATTSAYRVDLRRAEYGDE
                     RDPQMKAFFERISPAGQADKIVSALLVAHGRNDPRVPFSEAEQIASLVRGRGRTVWTV
                     FADNEGHGFGKKDNADYLRAVEAYFLKTSLGID"
     gene            complement(1115..3028)
                     /locus_tag="VFB80_21265"
     CDS             complement(1115..3028)
                     /locus_tag="VFB80_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36379.1"
                     /translation="MPRHRFFVRLFSGVLVLSAAAAALAVQPSEKLLPATTKGFISTH
                     DVDEVRTKFNETQFGKLVHDPLMEAFIADFKKQIGKKLEKAGKKLGIQWDDMEGVYAG
                     EVALGLVQPDPKDKNSHATVLIVDITGKRGQADVLLAKIDANQKANKAVKGAVAVGDV
                     TLTSYKQPLQEGEKVAEVAYYFIKDNQLVATDHLNVAKEIVGRFNGRATDTLESVKAF
                     DHTMKENAKLAGGVRHHVRWFVEPLGYLETARAAEGGRKKRGTDPLKILQGEGFTAVQ
                     GVGGHVFFGLNGHEITHRTYAYAPPVVRKKDDPSKDKYNLSMRMLDFPNSAKGALEPQ
                     PWAMSDLATYLSFNWKTQDAFKYSESLVDAIIADKGAWQEMWKGMKEDEFGPQIDIFK
                     ELVDHLGERVTVLVDVKLPVGLKSERLLGLVEVNKPEIIAKTLDKAFKKDPTAKKHVY
                     KGQVIWELTQEELAGDTELMIEGAGFVSAPEKKKDEEKDKALPNMAITVFAGHLVIGT
                     HLDFVQELIDRAAAKPNLAVAADYLRVQDELVKLGSQEDSFHFFSRTDESYRATYELI
                     KQGKLPEAETLLARVLNALLGPTEEGVVREQEIDGAKLPDFDLVKKYFGPGGLYVQSE
                     ANGWRIVGCLLKK"
     gene            complement(3329..4552)
                     /locus_tag="VFB80_21270"
     CDS             complement(3329..4552)
                     /locus_tag="VFB80_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36380.1"
                     /translation="MSDLHRPSVSLARRQWMRDVAAVALPAAIAAAGSSLLGQEPKAQ
                     PPKRPASLQEFAGQGPAGAAAGAPADAAGCNDCGGLGFLPLKNRRPYFHVEGQPLPKA
                     ADAVPHRYCPTCRADGNDKDLVAEQTERFKTALGGHQKWEKETEFNLLRVETRHIEVH
                     GQFPAAEAIKIGQAFESLAAHLQTLTGTMELTQTRPDSFEMMALLEKPPMDHFRTVLE
                     RIYTVERLGEHWGLTRGVPAVDGFPLPFFYQTAEDTKRRPYAHGICFMGGRKLLRVAT
                     QERASRWLQEGFAEYCEFAALKANLWRTVYNDNPPPPMGSWAQQVRQLALGKQLRPWP
                     EQRKRELRDWDARDYLQSFGMVAYLLQSDPKKFLELTRKLRAGLEEDKALEESYAMPL
                     AALETACTKWLAGGK"
     gene            4957..5877
                     /locus_tag="VFB80_21275"
     CDS             4957..5877
                     /locus_tag="VFB80_21275"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36381.1"
                     /translation="MGIVPTPRPRQGFTLVELLVVIAIIGVLVALLLPAVQAAREAAR
                     RTSCANKMRQLALATHNLNDTYRALPPLVGSFPTNSNDKGTVFYFLLPYIEQKNFYDS
                     TKDAQGRQVASNIVQGSSPQIRAYGVFIPAFLCPSDVSNPSRQVRNPGTLNAWATASY
                     AANALVFVPDASIARTIPDGTSNTIMFVERYQICNGEWHYWGTFLNSNNPDPSPPKAP
                     WYRTPGITITGQPANPQTATSTAFQVGPKITGAATDPTVCNWATPNSPHSAMMVALAD
                     ASVRLFKKSMSLQNFQLATRADDGLTWTED"
     gene            6091..6888
                     /locus_tag="VFB80_21280"
     CDS             6091..6888
                     /locus_tag="VFB80_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36382.1"
                     /translation="MFPDLRELDRLITARPVSKIEQLLEAGGDQMNGGGTMRPAFTLQ
                     SIGWEDAVRSLTEDEKGRLLQQREGERLALLDWVRSGASRDAYSQDDYLPSGAAKIQQ
                     ITAAYRVSDPSADVPAAAARVRIRSLIADRCVTCHGENGRHDTARFIPLDSYEQLEPR
                     LRPESPGAQQKWVAVALVGLLPLALVFGVGYWHTSHPLLTRSFTLTLTGSALGVMLAC
                     WLLGGSGAWSGRAIAVAAALATLGTALHAFATLNDLLFDMRPPRDGA"
     gene            complement(6913..>12268)
                     /locus_tag="VFB80_21285"
     CDS             complement(6913..>12268)
                     /locus_tag="VFB80_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36383.1"
                     /translation="SYQAHTVEVQRPADGQALLLLKHVLRPYDETRIRINTAKNAVLS
                     IEFFDFGTLTGKMEAKDHVQVAGIWLPQRIEQTDEAGRPLRTVTRKHTVLTPEQFAAQ
                     FAERLKEQAEALRISPLFPALEKAKAAAAAGRATLEDRLVILHSLVWRQQWPAALAEL
                     ETIEKLIAGKRFAPWLRLWLLEQARRNEELRQLALALAPQIAMQARSGDFVLAQRLFA
                     YATNNLQAGEVLAVLDPLRPVFERQPEPARAVARWQSYRVSQLQAIGRLDEELALRRQ
                     LATQYPDDRDYQAEYARALKSGGDTEAAYAWLKAALAQPEAKWPASDRDYLYNVWAEM
                     LRDQGRWAELAELAAAWIEQRPENSDAYELYYAALVFTNRYDEADRKLAEWFAAGLAA
                     PLLTKTTEPPLYAAIETAQGENSYLRGGDEDPQWYGELAAVIETFASRPDQRGIVGNA
                     NGNWRFRQSEEFKAVSRKLVARLKAEAGTLPVGQLEQLADWVASYGLDVGVDDWKAVA
                     AAVRPRWEAATHRAIEQALAGLLTSCYSRIGAEERLAFLRLRLEKADDENRAGRAQEL
                     FEALLEQPWTAACEDEALALLDKLAAGDDATARLHQQILYVFRWVDRMEQARNELYER
                     EIKEPEKLTRTELAEKRKENRKRARSELSEKLAALAKTRQDGLARWLLVERLHLEIKL
                     ERDLEKVAESCFELLGAQPPAAKPADAELPLADALDLQFRTRLLTMLTYLASKRDAQA
                     AVTPRLEKYFDALIAQNPDGTLIGLDGRAGKYALLVVQDRPQELLAAVSAWPAQVKYP
                     QRWQRILAYLRAELGQLAEAVALAESLIKEDAWSGGDYRQLANWQQALGQKAKYEAAL
                     VAAYQQLDEYRLRRLLDQARAPWQNPNANATPGTIDPQVFLIQKALYQKASEPQHYVY
                     ISRSFYESSRDFRLLAGLADAVIGQSALNVYPFLGEMKTTLDEIDREAAADEMLRYIA
                     ALRGQGKTPVDQRALDLLEMLAERRAAEVQNQPGPHVQRAVAALQRAEKREWSPGEPR
                     LLAEMLGKLGGISQKPLADEVLRVMAALYAAEKEGTLDRLRISRWLAEALHNNGRRQE
                     GIDVLEVAIPEYRRAVGGKTTMEMVEPVALLSDLYSGADQFVRAEAVLRDEIPRAANQ
                     NVANDLVWRTYAVHNRALERRGTTSLGSGQALYQGLQAELQKAAAATPDHRQRRRLVE
                     ELSQFYRTANRLNFAAAPADARRFATELFPPILAKQVNDYQDLVSTIAGCLTEVAGKR
                     DALAFFIARMETEPRWLERQGEGGWRRFGSRIAELRHELLAIGDLEPRLLAIVLKELR
                     RDLTTRQSHNQSIYHEGHQYFWPEKRDDFARVAEDVLMTRRDDEAGLLYIAEYLFHGL
                     TLKDRAIEILLDAHRKKLLRDDGQSQLAHYLHQVQRWGEAVAILEGLVERHPEALGYR
                     TRLMFAYFQSKRPDAMLALLAATDKHFHEGERWSELVAAQLAEACFDCELFEQTVKYY
                     EEAIARRLEATGNRSAGDQRLAQYNAELAFAWAGLKNTEKAVDAAGAAIVGWGAAGDV
                     HLRNHGRMKPLDVLREVLKESPNLDAYLAEVDKQAAESQEDSAIIRKALGEVYFERME
                     FAKAAAQLKIAVELAPSDPAVHSKLVACYDELKNGPAAAEQMFAAVEVTRRDVELWAS
                     LATRLQTLGQADEAERARTTLVEMLPGETEGHAKLAVLRQEANRWDDAILHWREVATL
                     RKLEPAGLLSLAAAELHQKQKAAADATLKQLESTDWPARFHDELRERLPKLREQWRGL
                     "
BASE COUNT         1938 a         4156 c         3921 g         2253 t
ORIGIN      
        1 tccggcctgg ccgcattcac cgtcaacgcc gatggcgcga gccagctcta tctgttgacg
       61 gcgactgtag cccgcgactc cgtcgcgggc ggcccgccac ggagtggcgg gctacctttt
      121 cggccgcagc gccgcgagct gaaactgccg atcggtctgg tcgggtcgct ggagttctcg
      181 cccgacggca agcagctcgg ctttacgctg gccgctcccg atgcgccggc ggaggcctac
      241 tccctggaga tcgacagcgg ccggctgacg cgctggacct acagcgaagt cggcggactc
      301 gacccccaga cgttcgcccg tgccgagcga atccgcttcc cctcgttcga caagcgcgaa
      361 atccccggct ggtacttcaa gccgaagaac gcctcgcccc agcggaaggc gccggtgctg
      421 atccagattc acggcggccc ggagagccag gcgcagccgc tcttctccgg cacgacgcag
      481 ttctacttga acgagctcgg cgtggccgtg ctcctgccca acgttcgcgg ctcggcgggc
      541 tacggcaagt cgtaccttaa gctcgacaac gcccagctcc gcgaggacag cgtcaaggac
      601 atcggcggac tgctcgactg gatcgcccgg cagccggagc tcgacgcgtc gcgggtcgcg
      661 gtgagcggcg gctcgtacgg cgggttcatg gtgctctcgt cgttgacgca tttcggcgac
      721 cggatcaagg ccggcatcga cgtggtcggc atctgcaact tcaacacgtt cctggcgacg
      781 acctcggcct accgggtcga tctgcggcgg gccgaatatg gcgacgagcg cgacccgcaa
      841 atgaaggcgt tcttcgagcg gatcagtccg gccggccagg cggacaagat cgtctcggcg
      901 ctcctggtcg cgcacggccg caatgatccg cgggtgccct tcagcgaggc cgagcagatc
      961 gcgtcgctgg tccgcggccg cgggcggacc gtctggacgg tcttcgccga caacgaaggc
     1021 cacggcttcg gcaagaagga caacgccgac tacctgcggg ccgtcgaggc gtacttcctg
     1081 aagactagct taggaattga ttaaccgtaa ctgactactt cttcagcagg cagcccacga
     1141 tccgccagcc gttcgcttcg ctctgcacat agaggccgcc ggggccgaag tacttcttca
     1201 ccaggtcgaa gtcgggcagt ttcgcgccgt cgatctcctg ctcgcggacg accccttctt
     1261 cggtcggccc gagcaaggcg ttgagcacgc gggccagcag cgtctcggcc tcgggcagct
     1321 tgccctgctt gatcagttcg taggtcgccc ggtacgactc gtcggtgcgg gagaagaagt
     1381 ggaagctgtc ctcctgcgag ccgagcttta ccagctcgtc ttgcacgcgg aggtagtcgg
     1441 cggccaccgc caggttgggc ttcgccgcgg cgcggtcgat cagctcctgc acgaagtcga
     1501 ggtgcgtgcc gatcaccagg tgtcccgcga agacggtgat cgccatgttt ggcagcgcct
     1561 tgtctttctc ttcgtccttt ttcttttccg gtgcgctcac aaacccggcc ccctcgatca
     1621 tcagctccgt gtccccggcc aactcttcct gcgtcagttc ccagatcacc tgccccttat
     1681 agacgtgctt cttggccgtc gggtccttct tgaacgcctt gtcgagcgtc ttggcgatga
     1741 tctcgggctt attgacctcc accaggccca gcagccgctc gctcttgagg cccacgggca
     1801 gcttgacatc gaccagcacc gtaacccgct cgccgagatg atccaccagc tccttgaaga
     1861 tgtcgatctg ggggccgaat tcgtcctcct tcatcccctt ccacatctcc tgccaggcgc
     1921 ccttgtcggc gatgatcgca tcgacgagcg actcggagta tttgaacgcg tcctgcgtct
     1981 tccagttgaa cgacaggtag gtcgccaggt cggacatggc ccaaggctgc ggctcgagcg
     2041 cgcccttggc gctgttgggg aaatccagca tccgcatcga gaggttgtac ttgtccttgc
     2101 tcggatcgtc cttcttgcgc acgaccggcg gagcataagc gtaagtgcgg tgcgtgatct
     2161 cgtggccgtt cagcccgaag aagacgtggc cgccgacgcc ctgcaccgcg gtgaagccct
     2221 cgccctggag aatcttcaag ggatcggtgc cgcgcttctt gcggccgcct tccgccgcgc
     2281 gggccgtctc aagatagccc agcggctcca cgaaccagcg gacgtgatgc cgcactccgc
     2341 cggccagctt ggcgttctcc ttcatcgtgt ggtcgaacgc cttgacgctc tccagcgtgt
     2401 ccgtagccct gccgttaaac cgcccgacga tctccttcgc cacattcagg tggtcggtgg
     2461 cgaccagctg attgtccttg atgaagtaat aggcgacctc cgcgaccttc tcgccttcct
     2521 gcagcggctg cttgtacgag gtgagcgtca cgtcgccgac cgcgaccgcg cccttgaccg
     2581 ccttgttggc cttctggttg gcatcgatct tggccagcag gacgtcggcc tggccccgct
     2641 tgccggtgat gtccacgatc agcacagtcg cgtgcgagtt cttgtccttg gggtcgggct
     2701 gcacaaggcc cagcgcgact tcgcccgcat agacgccctc catgtcgtcc cactggatgc
     2761 cgagcttctt cccggccttc tcgagcttct tgccgatctg cttcttgaag tcggcgatga
     2821 acgcctccat cagcgggtcg tggaccagct tgccgaactg cgtctcgttg aatttcgtcc
     2881 gcacctcatc gacgtcgtgc gtggaaatga agcccttcgt tgtcgccggc aggagcttct
     2941 cgctgggctg cacggccagc gccgcagccg ccgcagacag cacgaggacg cccgagaaca
     3001 agcggacgaa aaaccgatgg cgcggcacgg gaaggctcct tcgcgggtgg ctggcagaag
     3061 cgggctacgg gagtggctag cgagcaggaa aagtcggccg gcggcgaggc ggaggtatga
     3121 tgtaggttta atatcggcat tcgcgcccta acaaacgacg ggaatagact tacttgccgg
     3181 ttcccgcgca gccgctgctg cttgagcagg cactgcatga tacgcaagtc tggcaagctg
     3241 ggaaacccgc ggcgaccttg cgggcggttc agcgagtaag cgactcgccg ttgcaagcca
     3301 cgtgccgatc tcgtggacgc gagggaagct atttgccgcc ggcaagccac ttggtgcagg
     3361 cggtctcgag ggcagccagg ggcatcgcgt acgactcttc gagggccttg tcttcctcca
     3421 aacccgcgcg cagcttgcgc gtcagctcga ggaacttctt gggatcggat tgcagcaggt
     3481 aggcgaccat gccgaacgat tgcaggtaat cgcgggcgtc ccagtcgcgg agctcgcgct
     3541 tcctttgctc cggccagggg cgcagctgct tgccgagcgc cagctggcgg acctgctggg
     3601 cccagctgcc catcggcggc ggcgggttgt cgttatagac cgtgcgccac aggttggcct
     3661 tgagggccgc gaactcgcaa tactcggcaa agccttcctg cagccagcgg ctggcccgct
     3721 cctgcgtcgc cacacgcagc agcttgcggc cacccatgaa gcagatgccg tgggcgtaag
     3781 gccggcgctt cgtgtcctcc gcggtctgat agaagaacgg gagcgggaag ccgtccacgg
     3841 ccggcacgcc gcgggtcagc ccccagtgct cgcccaaccg ctcgaccgta tagatccgct
     3901 ccagcaccgt gcgaaagtgg tccatcgggg gcttttcgag cagggccatc atttcgaagc
     3961 tgtcgggccg cgtctgcgtc agctccatcg tgcccgtcag cgtctgcagg tgcgcggcca
     4021 ggctctcgaa ggcctggccg atcttgatcg cctccgccgc ggggaactga ccgtggactt
     4081 cgatgtgccg cgtctcgaca cggagcaggt tgaactcggt ttccttctcc catttctgat
     4141 gtccgcccag ggccgtcttg aaccgctccg tctgttcggc gaccaggtcc ttgtcgttgc
     4201 cgtcggcccg gcaggtcggg caatagcggt gcggcacggc gtcggccgcc ttcggcagcg
     4261 gctgcccttc gacgtgaaaa tagggcctgc ggttcttgag tggcaaaaag cctagcccgc
     4321 cgcaatcgtt gcagccggcc gcgtcggccg gtgcgcctgc cgctgcgccg gctggcccct
     4381 ggccggcaaa ctcctgcagg ctcgccggtc gcttgggcgg ctgcgccttt ggctcctgcc
     4441 ccagcagcga agagccggca gccgctatcg ccgctggcaa tgcgactgcg gccacatcac
     4501 gcatccactg gcggcgagcg agcgaaacac tcgggcggtg caagtcggac atggccgggc
     4561 tcctgctaga caagtcgcgg ccgccattat gcgaagcacg ggtaactgaa atcaagcgcg
     4621 ggccggggac aggtccgctc gtcgcggagt ttgggttcaa ccaccctctc aaggaacaga
     4681 accccgacct ttccgtatta cgaattcgac aattcgcgat ttaatgcatc tagccgcggc
     4741 ccaggcggaa gtaagaacgt ggggtagacc tggcaggggc ggtaaggggg accagcccgt
     4801 aatttcgcgc accatctcgg caagcgctgc tgtaagtcag aggcgatgga gttattccgt
     4861 ggccttcagc catttggcgc gctccgcgaa agccgcgcgg ggctcgtcga tcgcccacgc
     4921 caatgacttg cccgcgcgaa actctggaga ttagccatgg gaatcgttcc aacgccgcga
     4981 ccacggcagg gatttacact ggtcgaatta ttggtcgtaa tcgcgattat tggtgtgctc
     5041 gtcgcgttgc tgctgccagc cgtccaagcc gcccgtgagg cggcccgccg cacctcctgc
     5101 gccaacaaaa tgcggcaact ggctttggca actcacaatc tgaacgacac ctaccgcgcc
     5161 ttgccgcctc tggtgggcag ttttcccacg aacagcaatg acaaggggac ggtattctat
     5221 ttcctgctgc cctatatcga gcagaagaac ttctacgaca gcaccaaaga tgcccagggc
     5281 cggcaagtcg cctcgaatat cgttcaaggc tccagtccgc aaatccgggc ctacggcgtt
     5341 ttcatcccgg ccttcctctg tccatcggac gtcagcaacc cgtctcgaca ggtgcggaac
     5401 ccaggcacgc tcaacgcctg ggccactgcg tcgtacgcgg ccaacgcgct cgtcttcgtt
     5461 cccgacgcga gcattgccag gacaatcccg gacggcactt cgaacacgat catgtttgtg
     5521 gagcgctatc aaatctgcaa cggtgaatgg cattattggg ggacgttcct gaattccaac
     5581 aaccccgacc ccagcccgcc caaggcgcct tggtaccgga cgcctggaat tactattacg
     5641 ggacaacctg ccaacccaca gacggcaacg agcactgcat ttcaagtcgg ccccaaaatc
     5701 acgggcgcgg ccaccgatcc gaccgtatgc aattgggcca ctcccaattc accgcactcg
     5761 gccatgatgg tcgctttggc cgacgccagc gttcgcctgt tcaaaaaaag catgagcctg
     5821 caaaattttc aactcgccac gcgcgccgac gatggtctga cgtggaccga ggattagtcg
     5881 cagcgcccca ccgctcacgg tgcagctacc gacgccgcag cgctgcggcc gtaaggatcc
     5941 ttgcacttgg ctgctacgtt cacacgctcg cggctctgcc agctcccctg ggcgacgaga
     6001 cgagcgttgg gattgctcct gaccgcagtt gcaatcggat atggctccct cctcgtcgca
     6061 gtctattttc aactcacgcc tcccagttct ttgttccccg atctgcgcga gcttgatcga
     6121 ttgatcaccg ccaggccagt cagcaagatt gagcaactgc tcgaggccgg cggcgatcag
     6181 atgaacggcg gcggaacgat gcgccccgca ttcacccttc aatcaatcgg ctgggaggat
     6241 gctgtcagga gcctgactga ggacgaaaag ggccgattgc tccaacagcg cgagggcgag
     6301 cgcctggccc tgctcgactg ggtccgatca ggcgccagcc gcgatgcata ctcccaagac
     6361 gattatctgc cgagcggcgc ggcgaagatc cagcaaatca cggcggctta ccgggtgagc
     6421 gacccctccg cggatgtccc cgcggcagcc gctcgcgtgc gaattcgctc gctcattgcc
     6481 gaccgctgcg tgacctgcca cggtgaaaac ggcaggcatg acacggcgcg gttcataccg
     6541 ctggatagct acgagcagct cgagccgcgc ctgcggccgg aatcgccagg cgcccaacaa
     6601 aagtgggtcg ctgttgcgct cgtcggtctc ttgccgttag ccctcgtttt cggtgtcggt
     6661 tattggcata ccagccatcc actcctcacg cgatcgttta ctttgacgct caccggcagc
     6721 gcgctcggtg tgatgctggc gtgctggctg ctgggcggaa gcggcgcatg gtctggacgc
     6781 gcgattgcag tggccgcagc cctggcaacg cttggaaccg cgctgcatgc gttcgcgacg
     6841 ctgaatgatc tgctgtttga catgcggccg ccgcgggatg gagcctagcc cagccgggcc
     6901 atcccacgga tgctataggc cacgccattg ttcgcgaagc ttcggcagtc tttcgcgcag
     6961 ctcgtcgtgg aaccgggcgg gccagtcggt ggattcgagc tgcttcagcg tggcgtcggc
     7021 ggcggccttc tgcttttgat gcagctcagc ggcggccagc gacagcaggc ccgcgggctc
     7081 cagcttgcgg agcgtggcga cctcgcgcca gtgcaggatc gcgtcgtccc agcggttggc
     7141 ttcctggcgg agaaccgcca gcttcgcgtg cccctcggtc tcgccgggca gcatttccac
     7201 cagcgtcgtg cgggcccgct cggcttcgtc ggcctggccg agcgtttgca gccgcgtggc
     7261 caggctggcc cacagctcca cgtcgcggcg ggtcacttcc acggcggcaa acatctgctc
     7321 ggccgcagcg gggccgttct tgagttcgtc gtagcaggcg accagcttgc tgtgcacggc
     7381 ggggtcgctc ggcgcgagct ctaccgcgat cttgagctgc gcggccgcct tggcaaactc
     7441 catccgctcg aaatagacct cgcccagtgc cttgcggatg atcgcgctgt cctcctgcga
     7501 ctcggctgcc tgcttatcga cctcggccaa ataggcatcg aggttcggcg actccttgag
     7561 cacttcgcgg agcacgtcga gcggtttcat ccggccgtga ttccgcaaat ggacatcccc
     7621 ggcagcgccc cagccaacga tcgccgcgcc ggcggcgtcc acggccttct cggtgttctt
     7681 caggccggcc caggcgaagg ccagctcggc gttgtactgc gccagccgct ggtcgccggc
     7741 ggagcggttg cccgtggcct ccaggcggcg ggcgatggcc tcttcgtagt atttgacggt
     7801 ctgttcgaac agctcgcagt cgaagcaggc ttcggccagc tgcgccgcga ccaattcgct
     7861 ccaccgctcg ccttcgtgga agtgtttgtc ggtcgccgcg agcagcgcca gcatcgcgtc
     7921 gggccgcttc gactggaaat aggcgaacat caagcgcgtg cggtagccga gggcctcggg
     7981 atgccgctcc acgaggccct ccaggatcgc caccgcctcg ccccaccgct ggacctgatg
     8041 gaggtagtgg gcgagctgcg actggccgtc gtcgcggagc agctttttcc ggtgggcgtc
     8101 gagcaggatt tcgatcgccc ggtccttcag cgtcaggccg tggaacaggt actcggcgat
     8161 gtagagcagc ccggcctcgt cgtcgcggcg cgtcatcagc acatcttcgg cgacgcgggc
     8221 aaagtcgtcc cgcttctccg gccagaaata ctggtggccc tcgtgataga tcgactggtt
     8281 gtgcgactgc cgcgtcgtca gatcgcggcg cagctccttg agcacgatcg ccagcagccg
     8341 cggctccaag tcgccgatgg ccaagagctc gtgccgcaac tcggcgatcc gcgagccaaa
     8401 ccgccgccag ccgccttcgc cctgacgctc gagccagcgg ggctcggttt ccatccgggc
     8461 gatgaagaat gccagggcgt cgcgcttgcc ggcaacctcg gtcaggcaac ccgcgatcgt
     8521 gctgaccagg tcctggtaat cgttgacctg tttcgccagg attggcggaa acagctcggt
     8581 ggcaaaacgc cgcgcgtcgg ccggcgcggc ggcgaagttc aggcgattcg cggtgcggta
     8641 gaactgcgac agctcttcca ccagccgccg ccgctggcgg tggtcgggcg tggcggccgc
     8701 cgccttttgc agctcggcct gcagcccctg atagagcgcc tggccgctcc ccagcgaggt
     8761 cgtgccgcgg cgctccaggg cccggttgtg aaccgcgtac gtccgccaga ccaggtcatt
     8821 ggccacgttc tggttcgcgg cccgcggaat ttcgtccctg agcaccgcct ccgcccgcac
     8881 gaactgatcc gccccggaat agaggtccga gagcagcgcc acgggctcca ccatttccat
     8941 ggttgtcttg cctccgacgg cccgccggta ctcgggaatt gccacctcca agacgtcgat
     9001 cccctcctgg cggcggccgt tgttatgcaa tgcctcggct agccagcggc tgatccgtag
     9061 ccggtcgagc gtgccctctt tctcggcggc atagagcgcc gccatcacgc ggaggacttc
     9121 gtcggccagc ggcttttgcg aaatcccgcc tagcttgcca agcatttccg ccaggagccg
     9181 cggctcgccg ggcgaccact cgcgcttctc ggcccgctgc agggcagcga ccgcccgctg
     9241 gacgtgcggc ccgggctggt tctgcacttc ggcggcccgc cgctcggcca gcatctcgag
     9301 caggtccagc gcccgctgat cgacgggcgt tttgccttgc ccgcgaagcg cggcaatgta
     9361 ccgcagcatc tcgtcggccg ccgcctcgcg gtcgatctcg tcgagcgtgg tcttcatctc
     9421 gccgaggaac ggatagacgt tcagggccga ctggccgatg accgcgtcgg ccaggccggc
     9481 cagcaggcgg aagtcgcgcg acgattcata gaacgaccgc gagatataga cgtagtgctg
     9541 cggctcgctg gccttttgat agagggcctt ctgaatgagg aacacctgcg gatcgatcgt
     9601 gcccggcgtc gcattggcgt tggggttctg ccacggagcg cgggcctgat cgagcagccg
     9661 gcggaggcgg tattcgtcga gctgctggta ggccgcaacc agcgccgcct cgtatttcgc
     9721 cttctggccg agggcctgct gccagttggc gagctggcga tagtcgccgc cgctccaggc
     9781 gtcctctttg attagcgact cggccagagc gaccgcttcg gccagctggc ccagctcggc
     9841 gcgcaggtag gcgaggatcc gctgccagcg ttgcggatac ttcacctgcg ctggccaggc
     9901 gctgaccgcc gccagcaatt cctgcggccg gtcctgcacg acgagcagcg cgtacttgcc
     9961 cgcccggcca tcgaggccga tcagcgtgcc atcgggattc tgggcgatga gcgcgtcgaa
    10021 atacttctcc agccgcggcg tgacggccgc ctgcgcgtcg cgcttcgacg ccagataagt
    10081 cagcatcgtc agcagccggg tccggaactg aagatccagc gcgtcggcca agggcaattc
    10141 ggcgtcagcg ggcttcgcgg ccggcggctg agcgccgagc agctcgaagc aggactcagc
    10201 gactttctcc aggtcgcgct ccagcttgat ctccagatgc aatcgctcga cgagcagcca
    10261 gcgcgccagg ccgtcctgcc tggtcttggc cagcgcggcc agcttctcgg acagctcgct
    10321 gcgggcccgc ttgcggtttt ccttgcgctt ctcggccagc tccgtccgcg tcagcttctc
    10381 gggctccttg atctcgcgct cgtacagctc gttgcgggcc tgctccatcc gatcgaccca
    10441 gcggaagacg tacaggattt gctgatgcaa tcgtgcggtg gcatcgtcgc cggcggcgag
    10501 cttatccagc agcgccagcg cttcgtcctc gcaggcggcg gtccagggtt gctcgagcag
    10561 ggcctcgaat agctcttgcg cgcgaccggc gcggttctcg tcgtcggcct tttccagccg
    10621 caggcggagg aacgccagcc gctcttccgc cccaatgcgg gaatagcagc tcgtcagcag
    10681 gccggcgagg gcctgctcga tcgccctgtg cgtggcggcc tcccaccgcg ggcggaccgc
    10741 ggccgcgacg gctttccagt cgtccacgcc gacgtcgagg ccataggaag cgacccaatc
    10801 ggcaagctgc tcgagctgac cgaccggcag cgtgccggct tccgccttca gccgggcgac
    10861 cagcttgcga ctgaccgcct tgaactcctc gctctgccgg aaacgccagt tgccgttggc
    10921 attgccgacg atgccgcgct gatcgggccg gctggcgaag gtttcgatca cggccgccag
    10981 ctcgccgtac cactgcgggt cttcgtcgcc gccccgcaaa tagctgtttt cgccctgagc
    11041 cgtctcaatc gcggcgtaga gcggcggctc ggtggtcttg gtcaagagcg gcgcggccaa
    11101 acccgccgcg aaccactcgg ccagcttgcg gtcggcctcg tcgtagcgat tcgtgaagac
    11161 cagcgccgcg taatacagct cgtaggcgtc ggaattctcc ggccgctgct cgatccaggc
    11221 cgcggccagc tccgcgagtt cggcccagcg gccctggtcg cggagcattt cggcccacac
    11281 gttgtagaga tagtcgcggt cgctcgccgg ccacttcgcc tccggctgag ccagcgcggc
    11341 cttgagccag gcataggcgg cctcggtgtc gccgccgctc ttgagcgcgc gggcatactc
    11401 cgcctgatag tcccgatcgt ccggatactg cgtcgccagc tgccgccgca gggcgagctc
    11461 ttcatcgagc cggccaatcg cttgcagctg cgagacccgg tacgactgcc accgcgccac
    11521 ggcccgcgct ggctcgggct gccgctcgaa gacaggccgc agcggatcga ggaccgccag
    11581 cacttcgccg gcctgcaggt tgttcgtggc ataggcgaac agccgctggg ccaggacaaa
    11641 gtcgccggag cgagcttgca tggcaatttg cggggccagc gcgagggcca gctgccgcag
    11701 ctcttcgttg cggcgggcct gctccagcag ccacagccgc agccaggggg caaaccgctt
    11761 gccggcgatt agcttttcga tcgtctccag ctcggccagg gccgcgggcc actgctgtcg
    11821 ccagaccagg ctgtgcagga tgaccagccg gtcttcgagc gtggcccggc cggctgccgc
    11881 cgcggccttg gccttttcga gcgccgggaa gagcgggctg atccgcaggg cctcggcttg
    11941 ctctttcaat cgctcggcga attgagccgc aaattgctcg ggcgtcagga ccgtgtgctt
    12001 gcgcgtgacg gtgcgcagcg gccggccggc ctcgtcggtc tgctcgatcc gctgcggcag
    12061 ccagatgccg gcgacctgca cgtggtcctt ggcttccatc ttgccggtca gcgtgccgaa
    12121 atcgaagaac tcgatcgaca gcacggcgtt cttggccgtg ttgatgcgga ttctcgtctc
    12181 gtcgtatgga cggaggacgt gcttgaggag gagcagcgcc tggccgtccg ccggccgctg
    12241 gacctcgacc gtgtgcgcct gataggac
//
LOCUS       DASRJB010000813         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2763129, whole genome shotgun sequence.
ACCESSION   DASRJB010000813 DASRJB010000000
VERSION     DASRJB010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763129"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFB80_21290"
     CDS             <1..681
                     /locus_tag="VFB80_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36384.1"
                     /translation="LWYLLTGKVVYRGATVLEKLVAHREQPIPSLAAARAGVSPELDA
                     VFHRMVAKKPQQRYPNMTALIADLERCRLDPCASPTVSIDPAQGQNLQAFLEGIQSPK
                     SPRGAPPPAARPAAASIVAPPRQTTVPVAADTVFTSDSSTDTGPVAQAESPGLPQFAL
                     APRTSPKPPPAPRAKPRLRKPNSTQAIVVAIFVAAAIAIGALLYALREHLNFGPPAPQ
                     PPPVRTTA"
     gene            735..1451
                     /locus_tag="VFB80_21295"
     CDS             735..1451
                     /locus_tag="VFB80_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZN36385.1"
                     /translation="MPYSDFDPESLAAYLHLAPQQVLKMAERGSVPGRKIGGQWKFSQ
                     AEIHHWLEERIGAGDDAEIAQVQGLLDKAAEQKEPATVRISELLLPEAIAIPLDARTR
                     SSVIKSMVELAAKTGHLWDAAEMTEAVEAREKLHPTALDSGVALLHPRRPLGQILAQP
                     LLALGRTQRGIPFGSERGQLTDLFFLICSVDDSQHLRTLTRLSRLISTAGFLEELRAA
                     DSAEQALSAVRKFERSEEEP"
     gene            1448..2227
                     /locus_tag="VFB80_21300"
     CDS             1448..2227
                     /locus_tag="VFB80_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36386.1"
                     /translation="MSRNVHKPTIWLAGDWQHADFAAAIRWLGERACLLRLSLGEGTA
                     SPHAILVCQSRPGQTSQSAVEQWHSRAPLARLVALLGSWCEGELRSGRPWPGVMRVPW
                     RTWPVRLPLALDLESRSMRLPRTLTDVERLQRESAALRTQKNRAAKWTAIIRTESRAM
                     YDGISDALAALGMGSVWSAAAEALPAGCELEIIDGWKNVVDSPGPRRILLLHFPRPED
                     WTRAAAAGLAAVLPLPLHLAELAEAALRLAPQTATQQPAVA"
     gene            2325..3083
                     /locus_tag="VFB80_21305"
     CDS             2325..3083
                     /locus_tag="VFB80_21305"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZN36387.1"
                     /translation="MQLADLKWTDIDGLSRRTPVVIPIAALEQHGRHMPLFTDSLLLG
                     EVIRRVTERLKDRVLFTPLLWLGNSEHHLDFPGTMTAAPRAYLDILKDMVENFLVHNF
                     TRIVLVNGHGGNIVPAQQAMFEVRQKFRDRKGLALLSTTYWTLGGKPNEADKSLVQHR
                     MGHACEWETSMMLRLAPHLVGDYKAARPVVQTDRFEPAHRGWITKDRTEPGHIGDPRA
                     ASAEKGEVLFRTFAEDVTLFLQRVIDWDGASEVG"
     gene            3168..4532
                     /locus_tag="VFB80_21310"
     CDS             3168..4532
                     /locus_tag="VFB80_21310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN36388.1"
                     /translation="MSRDPGEPLSELTAAGRYIILSAAFLGWMFSGFQMAVMTLSARS
                     ATIEFLRSGQVSPDQLLRPGRLLASPRLRGVPPAPISGEPEALKIVVPTWFSWYNAAF
                     LFGAAGGGLVFGWLGDWMGRVRAMGLSIFWFSLFSAIGYVAATPEQLLASRFLAAMGV
                     GGMWPTGVSLASEAWSEGSRPLVAGMLGTSANVGLLALNVLGYIYNVGPDSWRWTLLV
                     CASPIVLAFLVWGFVPESRRWLAARRGAGAADSVSMGTVFRPPLLRLTLIGIALGTIP
                     LLGGWGATQWFIPWADKVGGAADPKAKALAGMMRSGGAVFGGLIGGWLANLGGRRLTY
                     FIVSLASLVLGEYIYLALSPNDRAFWGFVFVLGFVSTIFFGWLPLYLPELFPTHARST
                     GAGVSFNFGRILTALGVLGTGAITAWFHEDYGRAGSITTLVYALGMIVILFAPDTTKE
                     KLKD"
     gene            4624..>5387
                     /locus_tag="VFB80_21315"
     CDS             4624..>5387
                     /locus_tag="VFB80_21315"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN36389.1"
                     /translation="MSERPILILGGGINGAAAARELVLSGQSVWLVDAADLAFGATAY
                     SSRLIHGGLRYLEFAEFSLVKESLDERARLLRLAPHLVQPLRLFIPVRNSWGGLTQAA
                     GKFLGLPLGSKKSVHRGLRVVQVGLWLYDRYAAGGDLPPRSLHAPGDAGVPAVSPEVA
                     RKLWSYSDAQIRYPERMVIDLLYDARLIAGERGVDFRVFTYSRATLNGNAVELRPSDN
                     SASDPTATIEPAAIVNATGAWVDQTLARLPAPSRRL"
BASE COUNT          810 a         1827 c         1795 g          955 t
ORIGIN      
        1 ctctggtact tgctcacggg caaagtggtc tatcgcggcg cgacggtgct ggaaaaactt
       61 gtggcccacc gcgagcagcc gattccctcg ctggccgcgg cccgcgcggg cgtgtcgccg
      121 gaactcgacg ccgtgttcca ccggatggtc gccaagaagc cccagcagcg ctatccgaac
      181 atgaccgcgc tgatcgcgga cctggagcgg tgccgcctcg atccgtgcgc ctcgccgacg
      241 gtcagcatcg atcccgcgca gggccagaat ttgcaggcct tcctggaagg catccagtcg
      301 cccaagagcc cgcgcggtgc gccgccgccg gcagccaggc cagccgcggc gtcgatcgtc
      361 gcgcctccgc gtcagacaac cgttccggtc gccgccgaca cggtgttcac cagcgattcg
      421 tccaccgaca cgggtcccgt tgcccaggcc gagtcgccgg ggctgcctca atttgccctg
      481 gccccacgca ccagccccaa acctccgcct gcgccgcggg ccaagccccg tctgcgcaag
      541 ccgaactcca cgcaggccat cgtcgtggcg atcttcgtcg ctgcggccat cgcgatcggg
      601 gcgctcctct atgcccttcg cgagcacctg aatttcggcc cgcctgcgcc gcagccgcct
      661 cccgtgcgga cgacggcctg aagggccgcc ggattgatct ggacggctcg cggcgacctg
      721 ccgacaatct cgccatgcct tactccgatt tcgatcccga atcgctggcc gcctatttgc
      781 accttgcccc gcagcaagtg ctgaagatgg cggagcgcgg cagcgtgccg ggccgcaaga
      841 tcggcgggca gtggaagttc tcgcaggccg agattcacca ctggctggag gagcggatcg
      901 gggcgggcga cgacgcggag atcgcccaag tgcaagggct gctcgacaaa gctgcggagc
      961 agaaggagcc ggcgacggtg cggatcagcg agctgctcct ccccgaggcc attgcgattc
     1021 ctctggacgc ccgcacccgc agctcggtca tcaagtcgat ggtcgagctg gctgccaaaa
     1081 cgggccactt gtgggacgcg gcggaaatga ccgaggcggt cgaagcccgc gagaagctgc
     1141 atcccacggc gctcgacagc ggcgtggcct tgctccaccc gcgccggccg ctgggacaga
     1201 tcctcgcgca gccgctgtta gccctgggcc gcacgcaacg gggcattccg ttcggcagcg
     1261 agcgcgggca gctcaccgac ctgtttttcc tgatctgctc ggtcgatgac tcgcagcatt
     1321 tgcggacgct gacccggctg agccggctga tttcgacggc ggggttcctg gaggaactgc
     1381 gggctgcgga ctctgccgag caggcgctat ccgccgtgcg gaagttcgaa cgcagcgaag
     1441 aggagccatg agccgcaacg tgcacaagcc gacgatctgg ctggccggcg actggcagca
     1501 cgccgatttc gccgccgcga ttcgttggct cggcgagcgc gcctgcctgc tccggctctc
     1561 tctgggagag gggacggcca gcccccacgc catcctcgtg tgccagtcgc ggcccggtca
     1621 gacttcgcag tcggctgtcg aacaatggca ttcccgcgcg ccgctggccc gcctggtggc
     1681 actgctcggc agctggtgcg aaggcgaatt gcgcagcggc cgcccttggc cgggggtcat
     1741 gcgcgtgccc tggcgaacct ggccggtccg cttgccgctg gcgctggacc tcgagtcgcg
     1801 gagcatgcgc ctgccgcgga cgctcaccga tgtggagcgc ctccagcgag agagcgcagc
     1861 tctgcgcacg caaaagaacc gcgcggcaaa gtggacagcc atcatccgca ccgaaagccg
     1921 cgcaatgtat gacggcatta gcgacgccct cgcggcactc ggaatgggca gtgtgtggtc
     1981 ggctgcggcg gaggctcttc ccgcaggctg tgagctggag atcatcgacg gctggaagaa
     2041 cgtggttgat tcgcctgggc cgcgtcgaat cctgttgctg cacttcccgc ggccggaaga
     2101 ttggactcgc gcggcagcag caggcctggc cgccgtgttg cctctgccac tgcacctggc
     2161 ggagctggcg gaagccgccc tgcggctcgc tccgcaaaca gccacgcagc agccggcggt
     2221 cgcttgattg cctcgcagcg cggaatcacc taagctacct ccgcggacaa gactcttctg
     2281 cacgcaccgc tatttgggcc gacatttggg cggccatttg ggatatgcaa cttgccgatc
     2341 tgaagtggac cgacatcgat ggactcagcc gccggacgcc ggtcgtcatt ccgattgcgg
     2401 cgctggagca gcacggacgg cacatgccgc tgttcaccga cagcctgctc ttgggtgaag
     2461 tgatccgccg cgtgaccgag cgcttgaagg accgcgtcct gttcacgccg ctcttgtggc
     2521 tgggaaactc ggagcaccac ctcgattttc caggcacgat gactgccgct ccgcgggcct
     2581 acctcgacat cctcaaggac atggtcgaga acttcctcgt ccacaacttc acgcggatcg
     2641 tgctggtcaa cggccacggc ggcaacatcg tgcccgccca gcaggcgatg ttcgaagtgc
     2701 ggcagaagtt ccgcgaccgc aagggcctgg cgctgctttc gacaacttat tggacgctcg
     2761 gcggcaagcc gaacgaagcc gacaaatcgc tcgtgcagca ccgcatggga catgcctgcg
     2821 agtgggagac gtcgatgatg ctgcggctgg ccccgcacct cgttggcgac tacaaggcgg
     2881 cccggccggt cgtgcagact gatcgcttcg agccggccca ccgcggctgg atcaccaagg
     2941 accgcaccga gcccggccac attggcgacc cgcgggcggc ctccgccgag aagggcgaag
     3001 tgctgttccg caccttcgcc gaagacgtca ccctcttctt gcagcgggtg atcgactggg
     3061 acggcgctag cgaagtcggc tagccgtagc ataagcgtcc tcgcttgtgc aatgatcgtt
     3121 cgccggcaaa tgcgagcaag cgaggacgct tactctacga gtgtgccatg agccgcgatc
     3181 ctggcgagcc actgagcgaa cttaccgccg ccggccgcta catcatcctc tccgcggcgt
     3241 tcctgggttg gatgttctcc ggatttcaga tggcggtgat gacgctctca gctcgctcgg
     3301 ccacgatcga gttcctccgc agcggccagg tgtctccgga tcagctgctg cgtcccggcc
     3361 ggctgcttgc ctcgccgcgg ctgcgtggtg tgccgcccgc gccgatttcc ggtgagcccg
     3421 aggcgctcaa gatcgtcgtg ccgacctggt tctcgtggta caacgccgcg ttcctgttcg
     3481 gggcagccgg cggcgggctg gtcttcggat ggctcggcga ctggatgggc cgcgtacggg
     3541 cgatgggact ttcgatcttc tggttctcgc tgttttcggc aatcggctat gtcgcggcca
     3601 caccggagca gctgctcgcg tcgcgcttcc tggcggcgat gggcgtcggc ggcatgtggc
     3661 cgaccggcgt ctcgctcgcc tcggaagcct ggtcggaagg gtcgcgcccg ctcgttgccg
     3721 gcatgctcgg cacgtccgcc aacgtcggcc tgctggcgct caacgtcctg ggctacatct
     3781 acaacgtcgg ccccgattcg tggcgctgga cgctgctggt ctgtgcctcg ccgatcgtgc
     3841 tcgcgttcct ggtttggggg ttcgtccctg agtcgcggcg ctggctggcc gcccggcgcg
     3901 gggcgggcgc tgcggatagt gtgagcatgg gcacggtatt tcgcccgcca ctgttgcggc
     3961 tgacgctgat cggcatcgcc ctgggcacga ttccgctgct gggcggctgg ggcgcgacgc
     4021 agtggttcat tccgtgggcc gacaaagtcg gcggcgcggc cgatcccaag gccaaggcgc
     4081 tggccggcat gatgcgctcc ggcggcgcgg tcttcggcgg actgatcggc ggctggctgg
     4141 ccaacctcgg cggacggcgg ctgacctatt tcatcgtcag cctggcctca cttgtgttgg
     4201 gtgaatacat ctacctggcg ctgtcgccga acgatcgggc gttttggggc tttgtgttcg
     4261 tcctgggctt cgtttccacg attttcttcg gctggctgcc gctctatttg cccgagctgt
     4321 ttcccacgca tgcccgctcg acgggcgctg gcgtgtcgtt caacttcggc cgcatcctga
     4381 ccgcactggg cgtcctcggc accggcgcga tcaccgcctg gttccacgaa gactacggcc
     4441 gtgccggcag catcacgacg ctagtctatg ccctcggcat gatcgtgatc ctatttgcac
     4501 cggatacgac caaggaaaaa ctgaaagact aggcaacggt cggtcggtgg tcggttagtc
     4561 agttggtcgg tggtaatgca accgactgac cgatcgaccg actgaccgac ccacccagcc
     4621 accatgtcag agcgtccgat cctgattctc ggcggcggca tcaatggcgc ggcggcggct
     4681 cgcgagctgg tgctcagcgg ccagagcgtg tggctggtcg atgcggccga cctggccttc
     4741 ggggcgacgg cctactcgtc gcggctgatc cacggcggcc tgcggtacct ggagttcgcc
     4801 gagttttcgc tggtcaagga gtcgctcgac gagcgggccc gcctgctgcg actggccccg
     4861 catctggtcc aaccactgcg gctgttcatt ccggttcgca acagctgggg cgggctcacg
     4921 caagctgccg gcaagttttt gggcctgccg ctggggagta agaagtccgt acatcgcggc
     4981 ctgcgcgtcg tgcaagtcgg actctggctc tacgaccgct acgccgccgg cggcgacctg
     5041 ccgccccgat cgctgcacgc gccgggcgat gcgggcgtgc ctgccgtcag tcccgaggtc
     5101 gcccgcaagc tctggtccta cagcgacgcg caaatccgct accccgagcg gatggtgatc
     5161 gacctgctct acgacgcccg actgatcgcc ggcgagcggg gagtcgattt ccgggtcttc
     5221 acttactcgc gggccacact gaacggcaat gccgtggagc tgcggcccag cgacaattcg
     5281 gcgtccgatc cgactgccac gatcgagccg gcggcgatcg tcaacgctac cggggcatgg
     5341 gtcgatcaga ctttggctcg attgcccgcg ccgtcgcggc ggctgat
//
LOCUS       DASRJB010000814         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3905713, whole genome shotgun sequence.
ACCESSION   DASRJB010000814 DASRJB010000000
VERSION     DASRJB010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905713"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="VFB80_21320"
     CDS             <1..1979
                     /locus_tag="VFB80_21320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36390.1"
                     /translation="RRRVGPASPRAPAHHSAKEWWAGGRGGLVPPYGLRHMSNCPDSP
                     TLQAFAIGKLGGSEWQSVATHIEGCGDCLGRLDQLDAATDPLVEQLLQLPETPAETGA
                     AESESWASRVLVADAGRDLARRLDGGSVRLDRFEIQAELGVGSFGYVFRAWDPRLERN
                     VALKVQRAGSFVSTEEVQRFLREARSAAPLKHPAIVSLYETGHTEEGVGYLVCEFIDG
                     ETLETRLARGPLAPAEATELAAELADALDYAHEHGVIHRDIKPSNIILDRRGHPHLMD
                     FGLAKQDRGEASVTSDGRLLGTPAYMSPEQARGTPSDVDARSDIYSLGVVLFEMLTGR
                     RPFGGDRRQLLLAVMEEDPRPPRELNRNVPRDLEIICLKAMHKSPSGRYATARALADD
                     LARYRQAQPILARPLGFFERALRWCRRYPQAVSVLAAVLVGSVSGLVYLSSLAEYFVQ
                     QTALGGARLEAKMLDEVWRFYSQEIEDIKDVPITENYRTVHPSLPLPATFAIDLGERI
                     SQRTPGMKVRVYSRYPWPGRKDGGPQDEFDRLALTHLERHSRPSDNPPAEYSQFVAEG
                     GRRKLLFYTARHMEQSCLGCHNHPEGNSPKKDWKLGEVVGVLKIVRPLDDEIESTRRG
                     LRGAFVLMGSVAAALVVLSLAVTIVAQQRRKAAV"
     gene            1976..2596
                     /locus_tag="VFB80_21325"
     CDS             1976..2596
                     /locus_tag="VFB80_21325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36391.1"
                     /translation="MTAGANHRDGWATSTSRSLLAGARSEQPAAWVRLVNLYAPLVAA
                     WCRRLGVAEQDIADLLQDVFAAVARSLGRFRKESPEDTFRGWLATITRNKARDYHRRR
                     ADQPAAAGGTEATQRLAQVLDPEATQADAELADEVEMSAVLQRALSQIRGEFQERTWQ
                     AFWSVVVEGRAAADVAAAMNMQAGAVRVCKSRVLMRLRRELGDVPE"
     gene            complement(2694..>3540)
                     /locus_tag="VFB80_21330"
     CDS             complement(2694..>3540)
                     /locus_tag="VFB80_21330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36392.1"
                     /translation="YKSATRAIALLDELKDKPFFLAVGFSKPHSPPTAPAKLFDLYDA
                     ARMPLSPDFAALPAAPEGFPEASIPKRSGDLFIGREASREAAQEVVEAYYASTTFVDR
                     QIGRVLDRVEKLGLRDKTVIVLFGDHGYHLGEKGKWSKHNSLFEVAARVPLMVCLPGV
                     AAGKASPRTVQLLDIYPTLCEACGLPLPAGLEGHSLVPLARDPQSAWQHPAYTVTKAG
                     QSFGQSVRTERWRYTEWDGAAGGAVLCDLDADPHEQKNLAADPQHTQVVDEMKKLLAR
                     LPARK"
BASE COUNT          559 a         1176 c         1259 g          546 t
ORIGIN      
        1 caagacgtag ggtgggacca gcgagtccgc gagcgccggc ccaccactca gcgaaggaat
       61 ggtgggccgg cggccgcggc ggccttgtcc caccctacgg cttgcgccac atgtcgaatt
      121 gccccgacag cccgactttg caggcctttg ccatcggtaa gctcggaggc agcgagtggc
      181 agtccgtggc gacccacatc gaggggtgcg gcgattgcct gggccggctc gaccagctcg
      241 atgcggccac ggacccgctg gtcgagcagc tgctccagtt gccggaaact ccggcggaaa
      301 cgggggccgc cgagtccgag tcctgggcca gccgcgtcct ggtggccgac gccggccgcg
      361 acctggcccg ccggctggat ggcggcagcg tccggttgga ccggtttgag attcaggccg
      421 agctgggcgt cggctcgttt ggctatgtct tccgggcctg ggatccgcgg ctggagcgga
      481 acgtggcgct gaaggtgcag cgagctggca gcttcgtctc cacggaagaa gtccagcggt
      541 tcctccgcga ggcccgcagc gccgcgccgc tcaagcaccc ggcgattgtc tcgctctacg
      601 agaccggcca caccgaagaa ggcgtcggct acctcgtctg cgagttcatc gatggggaga
      661 cgctggagac ccggctggcc cgggggccgc tcgctccggc cgaggcgacc gagctggcag
      721 cggagctggc cgacgccctc gactatgccc acgagcacgg cgtgattcat cgtgacatca
      781 agccctcgaa catcatcctc gaccgccgcg gccatccgca cctgatggac ttcggactgg
      841 ccaaacaaga tcgcggcgag gcctcggtga cttccgacgg ccgcctgctc ggcacgccgg
      901 cttacatgtc gcccgaacag gcccgcggta cgccgagcga tgttgacgcc cgcagcgaca
      961 tctacagcct gggcgtcgtg ctgtttgaaa tgctcaccgg ccggcggccg ttcggcggcg
     1021 atcggcggca gctgctgctg gccgtgatgg aagaagaccc gcggccgccg cgcgagctga
     1081 atcgcaatgt gccccgcgac ctggagatca tctgcctcaa ggcgatgcac aagtcgcctt
     1141 ccggccgcta cgcgaccgcg cgggccctgg ccgacgacct ggcccgctac cggcaggcgc
     1201 agccaatcct cgcccgcccg ctgggctttt tcgagcgggc gctccgctgg tgccggcggt
     1261 atccgcaggc agtcagcgtg ctggcggccg tgctcgtcgg ctcggtctcc ggcctggtct
     1321 atctgtcaag cctggccgag tatttcgtgc agcagacggc cctcggcggc gcgcggctgg
     1381 aagccaagat gctcgacgag gtctggcggt tctacagcca ggagatcgag gacataaagg
     1441 atgttcccat caccgagaac tatcgcacgg tgcacccgtc gctgccgctg ccggcgacct
     1501 ttgcgatcga cctgggcgag cggatcagcc agcggacgcc gggcatgaag gtccgcgttt
     1561 acagccgcta tccctggccg ggccgcaagg acggcggccc gcaggacgaa ttcgaccggc
     1621 tggccctgac gcatctcgaa cgccattcgc ggcccagcga caacccgccc gccgagtatt
     1681 cgcagtttgt ggccgagggc ggccggcgaa agctgctctt ttacacggcc cgccacatgg
     1741 agcagagctg tctcggctgc cacaaccatc cggagggcaa cagcccgaaa aaggactgga
     1801 agctgggcga ggtggtcggc gtgctgaaga tcgtgcggcc gctcgatgac gagatcgaga
     1861 gcacgcgccg cggcctgcgg ggggcgttcg tgctgatggg ctcggtcgcc gcggccctcg
     1921 tcgtgctcag cctggcggtg acgatcgtcg cccagcagcg gcggaaggcg gccgtatgac
     1981 tgctggcgcc aatcaccgcg acggttgggc gacctccacc tcgcgcagct tgctggccgg
     2041 cgcgcggtcc gagcagccgg cggcctgggt gcggctggtg aatctctatg cgccgctggt
     2101 ggcggcctgg tgccggcggc tgggtgtcgc cgagcaggac attgccgact tgctccagga
     2161 cgtcttcgcg gctgtcgccc ggagccttgg ccggttccgc aaggagagcc cggaggacac
     2221 gtttcgcggc tggctggcga cgatcacgcg gaacaaggcc cgcgactatc atcgccgccg
     2281 ggccgatcag ccggcggccg cgggcggtac cgaagcgaca cagcggctgg cgcaagtgct
     2341 cgatccggaa gcgacgcaag cggacgccga actggccgac gaggtcgaaa tgagcgccgt
     2401 gctgcagcgg gcgctgtcgc aaattcgcgg cgaattccag gagcggacct ggcaggcgtt
     2461 ctggagcgtc gtcgtcgaag gccgcgccgc ggccgacgtc gccgccgcga tgaacatgca
     2521 ggccggcgcg gtccgcgtct gcaagtcgcg ggtgctgatg cggctgcggc gggagctggg
     2581 ggatgtgccg gagtagcgga aggaaaaacc gcggataacg cagatagcgc ggatagagag
     2641 gaaagcgaac aaacttctct catatctgcg tcatccgcga aatccgcggt tcctcatttc
     2701 ctcgccggca gcctggccag cagctttttc atctcatcca cgacctgagt gtgctgcgga
     2761 tcggcggcga ggttcttctg ctcgtgcggg tcggcgtcga gatcgcagag gaccgcgccg
     2821 ccggccgcgc cgtcccattc ggtgtagcgc caccgctcgg tgcggacgga ttggccgaaa
     2881 ctctggccgg ccttggtgac cgtgtaggcg gggtgctgcc aggccgactg cggatcgcgg
     2941 gccagcggca cgaggctgtg gccttccagc ccggccggca gcggcaggcc gcaggcctcg
     3001 cagagcgtgg gataaatgtc gagtaattgc acggtccgcg gcgacgcctt gcccgcggcg
     3061 acgccgggca ggcaaaccat cagcggcacg cgggccgcga cctcgaacag cgagttgtgc
     3121 ttcgaccatt tgcccttctc gcccaggtga tagccgtggt cgccgaacag cacgatcacc
     3181 gtcttgtcgc ggaggcccag cttctcaacc cggtcgagaa cgcggccgat ctggcgatcg
     3241 acgaacgttg tcgaggcgta gtaggcttcg acgacctcct gggccgcctc acgcgaggcc
     3301 tcgcggccga tgaacaggtc gccgctccgc ttgggaatcg aggcctcggg aaatccctcg
     3361 ggcgcggcgg gcagcgcggc aaaatccggc gacagcggca tcctggccgc gtcgtacaag
     3421 tcgaagagct tcgccggggc cgtgggcggg ctgtgcggct tgctgaagcc gacggccagg
     3481 aagaacggct tgtccttcag ctcgtccagc agggcgatgg cccgcgtggc cgatttgtaa
//
LOCUS       DASRJB010000815        13190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3616609, whole genome shotgun sequence.
ACCESSION   DASRJB010000815 DASRJB010000000
VERSION     DASRJB010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616609"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFB80_21335"
     CDS             complement(<1..491)
                     /locus_tag="VFB80_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36393.1"
                     /translation="MSQSPAQTTQPSAQSLRWPLRWHVALLVGLGIVAFASGIGGQFV
                     FDDFTSLIDDSQMHQVWPWGPISDANRWFGIWTLQWSYAIGGLDPRPYHLANIAIHLA
                     AGVTLLDLVRRSLLLPALAPRFGRWAAEIAVAVAAIWMVHPLQTQSVTYIVQRYESLM
                     GLC"
     gene            643..1326
                     /locus_tag="VFB80_21340"
     CDS             643..1326
                     /locus_tag="VFB80_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN36394.1"
                     /translation="MDTPFDVWIVVPAYNEGRRLSVTLRNLRRLYSNIVVVDDGSRDD
                     TGQFALAEGVWVLRHPINCGQGAALQTGIDFALQQGASAIVTFDADGQHSVDEISGLL
                     EPLRSGAADVVLGSRFLGRAEGLPFSRRLVLKAGVLFTRIFSRIRVTDTHNGLRALSR
                     HAAERIRLHENRMAHASEILHQIQLLRLRYCEQPVTIRYTAETLTKGQSSWNAISILS
                     QFILGRFVR"
     gene            1323..1658
                     /locus_tag="VFB80_21345"
     CDS             1323..1658
                     /locus_tag="VFB80_21345"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HZN36395.1"
                     /translation="MTPFQIVALSSLAALVLWEVARLRSAGFSGGSWLVRIGVWLAAA
                     AAVAQPQLLQQLANLLGIGRGTDVLLYLLVFAFFGTSFFLYARTIVLQRQLTQLVRQY
                     ALDNAQRRG"
     gene            1663..2343
                     /locus_tag="VFB80_21350"
     CDS             1663..2343
                     /locus_tag="VFB80_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN36396.1"
                     /translation="MLAREARWFRRQLERMPDGDVFPLLNVGSHTAEFRATGQPWIDG
                     ELFRPLADRGGRVVHTDIRPAAGVDLVGDLLDPAFRDELRQRRFRSVMFCNVLEHVSD
                     REPIARTVADVVAPGGLLLVSCPHRFPYHPDPIDTLYRPSVSELASLFPGTSLVHGQI
                     VPCGNLTTYVLGRASAAPVAFLRTMIQPRGVKSPHETAAPSSHKLALLPWLIRTFAVT
                     CVVLRKED"
     gene            2348..3508
                     /locus_tag="VFB80_21355"
     CDS             2348..3508
                     /locus_tag="VFB80_21355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN36397.1"
                     /translation="MRVLIVSHLALPHVGGVEVLVDREIRALAEAGHEVTLITSDGTG
                     QAQSPAYSPAVRIERVRAWHVLERHFRLPFPIFSPWLLAALWRASGAADVIHIHGCMF
                     LSSLAAALIGRLRGRPIILTDHGGIQQFDARWKSILARLGAETAGRITACCADRLVAY
                     NTRITRLLEQLTGRAGRAMFLPNPVDRSLFRPVDDACRQELRSRLGWTDGRPRVLFVG
                     RLTTEKGVPLLLECMDPSRYDLVFCGAGDSGLLGPLPRAGVEYRPPRPQRELAALYQA
                     ADLLVVPSKVREGFPLVVQEGLACGLKVVLGDDPGFAPYRRLTGLQLCQATVADVRRA
                     IVAALENPADFRPAAELAEFCPSAERWIERMYGDHARRSGPSRLAAAQTACP"
     gene            complement(3471..4466)
                     /locus_tag="VFB80_21360"
     CDS             complement(3471..4466)
                     /locus_tag="VFB80_21360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN36398.1"
                     /translation="MSLTCCQTPCLYCGGESFSPLYQGIRDRLGHVSGEWSFERCESC
                     GSAHLRPFPVAEQIAGFYPRVYTFAPQHAASPVQRWLAQLEFALFFRPVYRAQVRIID
                     RLVRPATSIARLLDIGCGQGLRLIEFRRRGYDCHGIDIDAAAVAAVQKQHGIPAVCGD
                     LATLEDHYPPQSFDVITAFCLLEHVPDVAGLLRQCFELLRPGGWMVGVVPLIDSAQIA
                     LFGRRDINLREAPRHLSIPTQQALRTACVRAGYAPDTFRLLPDTPLSCAGSAGLSLFP
                     SANTTHVYGSRRWWRVAWRLCGAASVAALAPWMLVENYLLKRPVMGMLFAQRPID"
     gene            4737..5459
                     /locus_tag="VFB80_21365"
     CDS             4737..5459
                     /locus_tag="VFB80_21365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN36399.1"
                     /translation="MDALRGPLASSFARQILTTCAPFLSKPVNELAVLDIGSGYGHTA
                     LALARQCKSVVGIEPNLPLWRSACALQQDAGQENLEFRHTGIYELDGSEQFDLIVLDN
                     VLEHLPDQPLALRIISDCLAPAGAAYILVPNRLWPLEVHYGLPFLSYLPLSWANRYLR
                     LSGRGTDYTDASYMPTYWRLNDLLMARRELSFQYVLPADIRLAAGGSAWHYRLGVRAI
                     RRWPWLWAISKALLVVVRKRVH"
     gene            complement(5464..6291)
                     /locus_tag="VFB80_21370"
     CDS             complement(5464..6291)
                     /locus_tag="VFB80_21370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN36400.1"
                     /translation="MTDELPVSVVMTVLEPHPRYFPEAVASVLAQTYTRWELVIVEDP
                     SASPAGELLRQFSDARIHHVCNPERTSHVRQRNQSLALARSELVATLDADDCCHPDRL
                     ARQVAFLREHPEIGVVGSQLAIIDAEGKRRFTRAYPCTPSGIVQAFPRYNPIAQPSVM
                     FRKSLIQAAGGYQYERYPAVEDYDLWSRLARRGVQFANLPDALLEYRLHQGGMKAMRL
                     KSILRGTLDVKQRYWMTALDWRGRGRLLLERLLLFVPPPLTYQLFQWTVLRRARPSR"
     gene            complement(6458..8515)
                     /locus_tag="VFB80_21375"
     CDS             complement(6458..8515)
                     /locus_tag="VFB80_21375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN36401.1"
                     /translation="MLASVAGVIAAAVAIRLLGVPQASAQAPAARQGAPAAQPRQPVG
                     SPAIRPAAPGGIPATAARPTAQPAPVAGSSAGGALAPTRPDPSTLQVMAVVNTEQITR
                     TELGRECIRRYGEEVLESLVNRMLIADACAKQGIKITDADISAEIDKIASRFGLSRDR
                     WLSLLREERGFTEAQYRTEVVWPMIALRQIAAAQIEVTPEELRKGFESEFGPKVKARL
                     IATDNQQKAAEAQRLAAANPKQFGELSKQKSTDPGVASAYGVIPPIRKNLGDANLERV
                     AFSLQPGQVSQVVNVANMYYVLLCEEHLPQQHLTSQQQAQQQARIREKIKENKLRIAA
                     GDFFDNVRKQAQVVNVYNDAAKAQQMPGVAATVNGRPVSLEQLAAECITRHGQEVLDG
                     EINRKILQQELNRKRLAVEPGEIDAEIARAAESYGYQKADGSPDVDKWLKSVTEVPGA
                     TVDLYVRDAVWPSCALKKLVGTKIDVSEEDLQKGFESNYGERVEVLAIVLSDHRQAQK
                     VWEQARNNNSDDFFGELAQQYSIEPASRGNKGKVPPIRRNSGSPMIEQEAFRLTAGEV
                     SGIVAVDDQFIIMRCQGRTKPIQVDYNKVRGELHKDILEKKLRMLMTREFDRLKDLAQ
                     VDNFLLGTSQTGGVKPANSMLLPSSPAAAAPRAPAATATAPRPAPAAAPINTQPRPVT
                     KSR"
     gene            complement(8723..9271)
                     /locus_tag="VFB80_21380"
     CDS             complement(8723..9271)
                     /locus_tag="VFB80_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36402.1"
                     /translation="MHKLKSISAAAVPAALEKALRYRLLNEPVEAESICLDILRVDPD
                     NTEAQVTLLLARTDLFDEEYVAALERAKAVLPLLTSEYDRAYYEGMIYERWAKAQAHR
                     KVPEHVVIGWHQHAMHCFERAQALAPPDNPDAILRWNTCARRLDREAAPVAADPSMTR
                     DVETEFGDETPSHLVTSSKNRA"
     gene            complement(9338..10102)
                     /locus_tag="VFB80_21385"
     CDS             complement(9338..10102)
                     /locus_tag="VFB80_21385"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZN36403.1"
                     /translation="MADKAERNSAEQATLRIYRGDSQQGEMVDYTVPIAPGMVVLDAI
                     HEVQARHASDLAVRWNCKAGRCGSCSAEVNGRPRLTCMDRLDKYPANEPIVVRPMKAF
                     PVIKDLACDVSWNYRVNKKIPAFELRTDRPRQFSQAEADRVQEFRKCIECFLCQNVCH
                     VLRDHDRQEEFCGPRFLVRVAGLEMHPLDAADRVKLLKQELGIGYCNITKCCTEVCPE
                     SIHITDNAIIPLKERVVDEFFDPVRKLLQIFNPTKK"
     gene            complement(10104..11912)
                     /locus_tag="VFB80_21390"
     CDS             complement(10104..11912)
                     /locus_tag="VFB80_21390"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZN36404.1"
                     /translation="MSKFQTHEYDVVVVGAGGAGLRAAIEASARGVRTALVCKSLLGK
                     AHTVMAEGGIAAALSNVDTRDNWQVHFRDTMKGGQFLNQWRMAQLHAQEAPERVRELE
                     EWGAVFDRTKTGDILQRNFGGHTYPRLAHVGDRTGLEMIRTLQDRGVHQGIDVHMECT
                     IRRVLLTAGRASGCMGYQRDSGRFVVFRAKAVVLATGGIGQCWEINSNSWEYTADGHA
                     MALWAGADLIDMEFVQFHPTGMVWPPSVRGTLITEGVRGEGGVLLNGERKRFMFQYIP
                     DRYKGEFAETEEEATRWVQAVIAGKRPEARRPPELLTRDVVARAIRAEVRAGRGTPHG
                     GVYLDIASRRTPDEIKRKLPGMYHQFKELADVDITKEPMEVGPTCHYMMGGVRVDPET
                     QAATVPGLFAAGEVGGGLHGANRLGGNSLSDLLVFGKRAGEFAAQRAREMPEWPRLDE
                     AEIDRIAKESLAPLERTAGENPYALHQELQQLMQRNVGIVRSAADLEEALGKLALLRQ
                     RSATVRCGGNIQFNPGWHLALDLANMLDISEAVVRAALERQESRAAHTREDFPDASPE
                     WGKLNLIVRQTAGGIEVHRQPLAALPPELDSILKGK"
     gene            complement(11916..12713)
                     /locus_tag="VFB80_21395"
     CDS             complement(11916..12713)
                     /locus_tag="VFB80_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36405.1"
                     /translation="MAAGAPAADRGGLGVTQRRDAWWAGPLATALALGAFGVYATARA
                     VMNARYHVGPGTGVLENHAYLLSPFYSPLIVLPWLPAWLSPAFLILWAPGGFRLTCYY
                     YRKAYYRAFFLDPPACAVGEARGKGYRGETGLFLFQNLHRYFLYFALIFLVILAIDVI
                     HSCIWPTAAGHSFGVSVATLVLAANVTLLSLYTFSCHSLRHLVGGNVDCFSCVALGNV
                     RQRAWSWASALNEHHMLFAWLSLFSVGFADFYVWMVASGRLTDLRIL"
     gene            12905..>13190
                     /locus_tag="VFB80_21400"
     CDS             12905..>13190
                     /locus_tag="VFB80_21400"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN36406.1"
                     /translation="MLASCAWGQGKKPDVALDGELSRLLVRHGFTGRIEEQLEARLGR
                     KLDPQLADLGRNLFFDPIMALRGDNSCAGCHAPQFGFADSQSIAIGIRNNN"
BASE COUNT         2172 a         4332 c         4311 g         2375 t
ORIGIN      
        1 aagcacaggc ccatcagcga ttcgtaccgc tgcacgatat aggtgacgga ttgcgtttgg
       61 agtggatgca ccatccagat ggcagccacg gccacggcga tttctgccgc ccagcggccg
      121 aagcgcggcg ccagagccgg caataacagc gagcggcgca ccaggtccag cagcgtcact
      181 ccggcagcca ggtgaatggc gatgtttgcc aggtgataag gccgcgggtc gaggccgccg
      241 atcgcgtagc tccactgcag tgtccagatg ccgaaccagc ggttagcgtc gctaatcggt
      301 ccccagggcc agacctggtg catctgcgaa tcgtcgatca gcgacgtgaa atcgtcgaag
      361 acgaattggc cgccgatgcc gctggcaaag gcgacgatac ccagaccaac gagtagtgcg
      421 acgtgccaac gcaggggcca gcgcagcgac tgggcgctcg gttgcgtggt ttgagcgggc
      481 gattgcgaca tcgagtttca gtttagttgc cccgatgaat cgccccaaac cgcgcctgca
      541 aagccggcac aatccgcacg cttcgctcgc aatgcggcgg attcgccgtg cgccaatcgc
      601 aaagcgctac tggcccggag ccaggcaatt agcgtggacc gtatggacac gcccttcgat
      661 gtctggatcg tcgtcccggc ctacaacgag ggtcgccggc tgtcggtcac gctccgcaat
      721 ctgaggcggc tgtattcgaa catcgtcgtt gtcgatgacg gctcgcgcga cgacactggc
      781 caattcgccc tggccgaagg cgtgtgggtg ctgcggcatc cgatcaattg cggccagggg
      841 gccgcgctgc agaccggcat cgatttcgcg ctgcagcagg gagccagcgc gatcgtcacg
      901 ttcgacgccg acggccagca ctccgtggac gagatctccg gcctcttgga gccgctgcgc
      961 agcggcgcgg ccgacgtggt tctcggctcg cgatttttgg gccgggcgga agggctgcct
     1021 ttcagtcgcc gtctggtgct gaaagccggc gtcctgttta cgcggatttt ctcgcggatt
     1081 cgcgtcaccg acacgcacaa cggactgcgg gccctctcgc ggcacgccgc cgagcgaatc
     1141 cgcctgcacg aaaaccggat ggcccacgcc tcggagattc tgcaccagat tcagctgctg
     1201 cggctgcgat attgcgagca gccggtgacg atccgctaca ccgccgagac gctcacgaag
     1261 ggccaatcga gctggaacgc gatttccatc ctcagccagt tcatcctggg gaggttcgtg
     1321 cgatgacgcc ttttcaaatc gtcgcgctaa gtagcctggc agcgctcgtc ctgtgggaag
     1381 tcgcccgcct gcgcagcgcc ggctttagtg gcggttcgtg gctcgtgcga atcggcgtct
     1441 ggctggcagc ggcagctgcc gtcgctcagc ctcagttgct tcagcaactg gcgaacctgc
     1501 tcggcatcgg ccgcgggacc gacgtgctct tgtacctgct ggtgttcgcc tttttcggca
     1561 catcgttctt cctctacgcc cgcaccatcg tcttgcagcg gcagctcacg cagctcgtgc
     1621 ggcagtacgc cctcgataac gctcagaggc gaggctaagg gcatgctcgc tcgcgaagca
     1681 cgctggttcc gcaggcagct ggagcgaatg ccggacggcg atgtgttccc gctgctcaac
     1741 gtgggcagcc acacggcgga gtttcgggcc acggggcagc cctggatcga cggcgagctc
     1801 ttccgcccgc tggcagaccg cggcggccgg gtggtgcaca ccgacatccg gccggccgcg
     1861 ggcgtcgatc tggtgggcga cctgctcgat ccggcgttcc gcgacgagtt gcggcagcgg
     1921 cggttccgct ccgtgatgtt ctgcaacgtg ctcgagcacg tctccgatcg cgagccgatc
     1981 gcgcggacgg tcgctgacgt ggtggcaccc ggcggtctgt tgctcgtgtc gtgcccgcac
     2041 cggtttccgt atcatccaga tcccatcgac accctctacc gcccaagcgt cagcgagctc
     2101 gcgtcgctgt tcccgggcac gtcgctcgtc cacggtcaga tcgtcccttg cggcaatctg
     2161 acgacctacg tgctcggccg cgcgtcggcc gcgccggttg cgttcctgcg cacgatgatt
     2221 cagccgcggg gcgtcaagag cccgcacgaa acggctgcgc ccagctcgca caagctcgcg
     2281 ctgcttccct ggctgatccg cacctttgcc gtgacctgcg tcgtgctgcg gaaagaggat
     2341 tgatcgcatg cgcgtgctca tcgtctcgca cctggccttg ccccacgtcg gcggcgtgga
     2401 agtgctcgtc gatcgcgaaa tccgcgcgct ggccgaggcg ggccacgagg tcaccctgat
     2461 caccagcgac ggcacgggcc aggcccagtc gccggcgtat tcgccggccg tcaggatcga
     2521 gcgcgtccgc gcttggcacg tgctggagcg gcatttccgc ctgcccttcc cgatcttctc
     2581 accttggctg ctcgccgcgc tgtggcgagc cagcggagcg gccgacgtca tccacattca
     2641 cggctgcatg tttctctcgt cactggcggc ggcgttgatc ggccgcctgc gcggcaggcc
     2701 gatcatcctg accgatcacg gcggcattca acagttcgac gcgcggtgga aatcgatcct
     2761 ggcacgtctt ggcgcagaga cggccggccg catcaccgcc tgctgcgcgg accggctcgt
     2821 cgcctacaac acgcggatta cgcggctcct ggagcagttg accggaagag cggggcgggc
     2881 gatgttcctg ccaaacccgg tggatcggag cctgtttcgc cccgtggatg acgcttgccg
     2941 ccaggagctg cggtcgcgac tgggctggac cgacggccgc ccgcgggtgc tgttcgtggg
     3001 acggctgacg accgagaaag gcgtgccgct cttgctcgaa tgcatggatc cttcgcggta
     3061 cgacctcgtt ttctgcggtg cgggtgatag tgggctgctg ggcccgctgc cgcgagcggg
     3121 tgttgagtat cgtccgccgc gtccgcagag agaactggct gctctttacc aggccgcgga
     3181 cttgctcgtc gtcccgtcga aagtgcgcga gggttttccg ctcgtcgtgc aggaaggact
     3241 ggcttgcggc ctgaaggtcg tcctggggga cgatccagga tttgccccat accgtcgcct
     3301 gacaggtctg caattgtgtc aggcgacggt cgccgacgta cggcgtgcga ttgtcgcggc
     3361 actggaaaac cccgctgact tccggcccgc ggccgaactg gctgaattct gcccgtccgc
     3421 ggagcgatgg atcgagcgaa tgtacggcga ccacgctcgc cgctcgggcc ctagtcgatt
     3481 ggccgctgcg caaacagcat gcccatgacg gggcgcttca gcaggtaatt ctcgacgagc
     3541 atccacggcg caagcgccgc cacgctcgcc gcgccgcaca accgccacgc gacgcgccac
     3601 caccgccggc tgccatagac atgcgtcgtg ttcgcagaag ggaacagcga cagtccggcg
     3661 ctgccggcgc agctgagcgg cgtatcgggc agcaggcgga acgtatccgg cgcatatccc
     3721 gcgcgaacac aggccgtgcg caaggcctgc tgggtgggaa tcgaaaggtg ccgcggcgct
     3781 tcgcggagat tgatgtcacg cctgccaaac agcgcgatct gtgcgctgtc aatcagcggc
     3841 accactccta ccatccagcc gccgggtcgc agcagttcga aacattggcg gagcagcccg
     3901 gccacatccg ggacgtgctc cagcaggcaa aacgccgtga ttacatcgaa cgactgcggc
     3961 gggtagtgat cttcgagtgt agccaggtcg ccgcagacgg ccggaatgcc gtgttgcttc
     4021 tgaaccgccg ccacggccgc ggcatcgatg tcgattccgt ggcaatcgta cccgcggcgg
     4081 cgaaactcga tcagccgcag gccctgcccg caaccgatgt ccagcaatcg ggcaatgcta
     4141 gtcgcgggcc gcacgagccg gtcgatgatc cggacctggg cccgatagac tggccgaaag
     4201 aagagtgcaa actccagctg agccagccag cgctggaccg gactggccgc gtgctgcggc
     4261 gcgaacgtgt agacgcgggg atagaatccg gcgatctgct ccgccaccgg aaacggccgc
     4321 aggtgggccg agccgcagct ttcgcagcgc tcgaacgacc actcaccgct cacgtgcccc
     4381 aggcgatcgc ggatgccctg atagagcggc gagaaactct cgcccccgca atacaggcac
     4441 ggcgtttggc agcaggtcag cgacataggc gcggcgagga taagagaccc ctaatcgacc
     4501 ctagtccgtt tgcccggtag ttcaattccg ctgccggatt gcccagcggc caatccggta
     4561 caaccggcaa atccagacgt tgcgatgggt caacaatctg gttcatccca tcaacatcgc
     4621 agcgctgagt gggtgctcct gctggggaat cggttttggc ccgccattcg caatcgcata
     4681 gcttcgtggc gacaacggtt ccggattacc gaccgcacta cgatcagttt cgcggcatgg
     4741 atgccttgcg cggcccgctg gccagcagct tcgcacggca gattctgaca acctgtgcgc
     4801 cgtttttgtc caagcctgtc aacgagctgg ccgtgctgga tatcggcagc ggctacggcc
     4861 acactgcgct cgcgctggct cgccagtgca aatccgtcgt cgggatcgag ccgaacctgc
     4921 cgctgtggcg cagcgcttgc gccctccagc aagatgccgg gcaggagaac ctggagttcc
     4981 gccacaccgg catctacgag ctggatggat cggagcaatt cgacctgata gtgctcgaca
     5041 acgtgctgga gcatttgccc gatcagccgc tggcgctgcg gatcatcagc gattgcctcg
     5101 ctccggcagg cgcagcgtac atcctggttc ccaatcggct gtggccgctg gaagtgcact
     5161 acggcttgcc cttcctgagc tatctgccgc tttcctgggc caatcgctac ctgcggctga
     5221 gcggccgtgg tacggactat accgacgcca gctacatgcc cacctactgg cggctgaatg
     5281 acctgctgat ggctcgccgt gagctgtcgt ttcaatacgt gttgcccgcc gatatccggc
     5341 tggcggccgg cggcagcgca tggcactacc gcctcggcgt cagggcgatt cgccgctggc
     5401 cctggctctg ggcgatttcg aaagccttgc ttgtcgtcgt gcgaaagcgc gtccattaac
     5461 ggatcagcgc gaaggtcgcg ctcgccgcag cacagtccat tgaaataact ggtacgtcag
     5521 cggcggcggc acaaaaagca gcagccgctc cagcagcaag cgtccgcggc cgcgccagtc
     5581 cagcgccgtc atccagtagc gctgcttcac atccagcgtc ccacgaagga tgctcttgag
     5641 gcgcatggct ttcattccac cctggtgcag gcggtactcg agcagggcgt cgggcaagtt
     5701 cgcgaactgc acgccgcgcc tggccagcct cgaccagagg tcgtagtctt cgacggccgg
     5761 ataccgctcg tattgatagc cgccggctgc ctgaataagc gacttgcgaa acatgacgct
     5821 cggctgggcg atggggttgt atctcggaaa ggcctggacg attcctgagg gcgtacatgg
     5881 atacgcgcgg gtgaatcgcc gctttccttc ggcatcgatg atcgccagct gcgagccaac
     5941 gacgccgatc tccggatgtt cgcgaaggaa tgccacctgc cgggcaaggc ggtcgggatg
     6001 gcagcagtcg tcggcgtcga gtgtggcgac gagttcgctg cgggcgagcg cgagcgactg
     6061 gttccgctgc cgcacgtgac tggtccgctc gggattgcag acgtggtgaa ttcgggcgtc
     6121 ggagaactgc ctgagcagct cgcccgccgg cgacgcagaa ggatcttcca cgatgaccag
     6181 ctcccaccgg gtgtaggtct gggcgagcac gctggccacc gcctctggaa aataccgtgg
     6241 gtgcggctcc aggacggtca ttaccacgga aaccggcaac tcgtcggtca tgcggcgcac
     6301 tcggtgggca ctcccacctt aattgccccg ccgtctaact gcggggcaat tcaccgcaat
     6361 cagctccttc agccgtgcag cggccgatac gaccactaga agcagaacaa gcggccgctg
     6421 ggccgcttgc tgctgtttca attcaaaaca ccgggagtta ccggctcttg gtcaccggtc
     6481 gcggctgcgt gttgattgga gcagccgccg gcgccgggcg cggggcggtg gcggtagccg
     6541 ccggtgctcg cggggcggct gccgcgggtg aagacggcag cagcatcgag ttggcgggtt
     6601 tgacgccgcc ggtctgcgac gtgccgagca ggaagttatc gacctgggcg agatccttga
     6661 gccggtcgaa ttcgcgggtc atcagcatcc gcagcttctt ctcgaggatg tccttgtgga
     6721 gctcgccgcg aaccttgttg taatccacct gaatcggttt cgtccgaccc tggcaacgca
     6781 tgatgatgaa ctggtcatcg acggccacaa tccccgaaac ctccccggct gtcaggcgga
     6841 atgcctcctg ttcgatcatc ggcgagccgc tgttgcggcg gatcggtggg accttgccct
     6901 tgttgcctct cgaggccggc tcgatcgagt attgctgggc caactccccg aagaaatcgt
     6961 ccgaattgtt gttgcgggcc tgttcccaga ctttctgagc ctggcggtgg tcgctgagca
     7021 cgatcgccag cacctcgacc cgctcgccat agttcgactc aaatcccttc tggagatcct
     7081 cttcgctgac gtcgattttc gtgccgacca gctttttgag ggcacacgac ggccagacgg
     7141 cgtcgcggac gtacagatcg accgtcgccc cgggaacctc ggtcaccgac ttcagccact
     7201 tatcgacgtc ggggctgccg tcggccttct gatagccgta cgattcggcc gcgcgggcga
     7261 tttcggcgtc gatctcgccc ggctcgacag ccagtcgctt ccgattgagc tcctgctgga
     7321 ggattttgcg attgatctct ccgtcgagca cctcctggcc gtgccgcgtg atgcactccg
     7381 cggcgagctg ctccagcgac acgggccggc cgttgacggt cgcagccacg ccgggcatct
     7441 gctgggcctt ggcggcatcg ttgtagacgt tcacaacctg ggcctgcttg cggacgttgt
     7501 caaaaaaatc gccggctgcg atgcggagct tgttctcttt gatcttctcg cggattcggg
     7561 cctgctgttg ggcctgctgc tggctcgtca ggtgctgctg cggcaagtgt tcctcgcaga
     7621 ggagcacgta gtacatgttg gcgacgttga cgacctgcga aacctgcccc ggctgcaggc
     7681 tgaaggccac ccgctccagg ttggcgtcgc cgagattctt gcggatcggt ggaatcacgc
     7741 cgtaggccga cgcgacgccc ggatcggtgc ttttctgctt cgagagttca ccgaactgct
     7801 tggggttggc tgccgccagc cgctgggctt cggccgcctt ctgctggttg tcggtcgcga
     7861 tcaggcgggc cttgaccttc gggccgaatt ccgactcgaa ccctttccgc agctcttcgg
     7921 gggtgacttc gatctgggcg gcggcgatct gccgcaaggc gatcatcggc cagacgactt
     7981 ccgtgcggta ctgcgcctcg gtgaagcctc gctcctcgcg gagcagcgac agccagcggt
     8041 cccgcgacag gccaaagcgg ctggcgatct tgtcgatttc cgccgagatg tcggcgtcag
     8101 tgatcttgat gccctgcttg gcgcaggcgt cggcgatgag catgcggttg accaggctct
     8161 cgagcacttc ctcgccgtag cggcggatgc attcgcggcc cagttcggtg cgcgtgattt
     8221 gttccgtgtt cacaaccgcc atgacttgca gcgtgctggg atcggggcga gtcggcgcga
     8281 gcgcgccccc ggccgaactg ccggcgactg gcgccggctg cgcggtcgga cgggctgccg
     8341 tcgccgggat tccaccgggc gctgccgggc ggattgccgg gctgccgacg ggctgccgcg
     8401 gctgcgcggc gggagcgccc tgccgcgcgg ccggcgcttg agcgctagcc tgcgggacgc
     8461 cgagcaggcg aatggcaacg gctgcggcaa tcacgccggc gaccgaggcc agcatccaca
     8521 gccgagcgcg ccagcgcgac ggggcagaat cattccgagt ctgggtttcc ataccgctca
     8581 tgggtcagtg ctccgtcgtc catgatccgg gaactcttcc gcgcggctcg gcaagaatgc
     8641 agccgccaaa gtttggggcg ggagtgtagg ccaagcgccg ccagcaatca agcgagaaaa
     8701 cagaccgccg ctagcaagcc agctaagcgc ggtttttgct ggaagtcacc agatgcgacg
     8761 gcgtctcgtc gccgaattcc gtctcgacgt cgcgggtcat gctcgggtcg gcggccaccg
     8821 gcgcggcctc gcgatccagc cggcgggcgc aggtgttcca gcgcaggatc gcgtcgggat
     8881 tgtccggcgg agccagcgcc tgggcccgct cgaagcaatg catggcatgc tggtgccacc
     8941 cgatgacgac gtgctcggga actttgcggt gagcctgtgc cttggcccag cgctcgtaga
     9001 tcatcccttc gtaataggcc cggtcgtatt cgctggtcaa gagcggcagc acggccttgg
     9061 cccgctccag cgccgcgacg tattcctcgt cgaacaggtc ggtgcgggcc aagagcagcg
     9121 tcacctgcgc ctccgtgttg tccggatcga cccgcagaat gtcgaggcag atgctctcgg
     9181 cctccaccgg ctcgttgagc agccggtaac gcagggcctt ttcgagggcc gcgggaacgg
     9241 cggcggccga aatcgatttc agcttgtgca tggctacctc gcgtagggtg ggctgtgccc
     9301 accggagttg gcctgatcat ggtgggcaca gcccacccta ctttttcgtc gggttaaaga
     9361 tttgcagcag ttttcggacc ggatcgaaga attcgtccac gactcgctcc ttgagcggga
     9421 tgatcgcgtt gtccgtgatg tgaatgcttt cggggcaaac ctccgtgcag cacttggtga
     9481 tgttgcagta gccgatgccc agctcctgct tcagcagctt cacgcggtcc gctgcgtcga
     9541 gcgggtgcat ctccagcccg gcgacgcgga ccaggaaccg cggcccgcag aattcctcct
     9601 gccggtcatg gtcgcgcagg acgtggcaga cattctggca caggaaacac tcgatgcact
     9661 tgcgaaactc ctgcacgcgg tcggcttcgg cctgtgagaa ctgccgcggc cggtcggtgc
     9721 ggagctcaaa cgcggggatc ttcttgttga cccgatagtt ccaggaaacg tcgcaggcca
     9781 ggtccttgat cacgggaaag gccttcatcg gccgcacgac gatcggctcg ttggccggat
     9841 acttgtcgag cctgtccatg caggtcagcc gcggacggcc gttgacctcg gcgctgcacg
     9901 aaccgcagcg gccggccttg caattccagc ggacagccag gtcggaagcg tggcgggcct
     9961 gcacctcgtg aatcgcgtcg agcacgacca tgccgggggc aatcggcacg gtgtaatcga
    10021 ccatttcgcc ctgctggctg tcgccgcgat aaatgcggag cgtggcttgt tcggcactgt
    10081 tcctctcggc cttgtccgcc atgtcatttt cccttgagga ttgaatcgag ctcgggcggc
    10141 agcgcggcca gcggctgccg gtggacttcg atgccgccgg ccgtttgccg cacgatcagg
    10201 ttcagcttgc cccactcggg gctggcgtcg ggaaagtcct cgcgagtgtg ggcggcgcgg
    10261 ctttcctgcc gctcgagggc cgcgcggacg accgcctcgc tgatgtcgag catgttcgcc
    10321 aggtcgagag ccaggtgcca accgggattg aactggatgt tgccgccgca gcggacggtc
    10381 gccgagcgct ggcgcaggag ggccagctta cccagcgctt cttccagatc ggcggccgag
    10441 cggacgatac ccacgttccg ctgcatcagc tgctgcagct cttggtgcaa ggcgtaagga
    10501 ttctctccgg cagtacgctc caagggagcg agcgactcct tggcgatacg gtcgatctcc
    10561 gcctcgtcca gtcggggcca ttcgggcatc tcgcgggccc gctgagccgc gaactctccc
    10621 gcccgtttgc cgaacaccag caggtccgag agcgagttgc ctcccaggcg gttcgcaccg
    10681 tgcaatccgc cgccgacttc gccggccgcg aacagtcccg gcacggtcgc ggcctgcgtc
    10741 tcgggatcga cccgcacgcc gcccatcatg taatggcacg tgggcccgac ttccataggc
    10801 tccttcgtga tatcgacgtc ggccagctcc ttgaactggt gatacatgcc gggcagcttg
    10861 cgcttgattt cgtccggcgt gcggcgcgag gcgatatcca gatagacgcc gccgtggggc
    10921 gtgccgcggc cggcccgcac ttccgcccgg atcgcgcggg ctacgacgtc gcgggtgagc
    10981 aattccggcg gccgccgggc ttcggggcgc ttgccggcga tgacggcttg cacccacctg
    11041 gtggcttcct cctccgtctc ggcgaattcc cccttgtagc ggtcgggaat gtactgaaac
    11101 atgaaccgct tccgctcgcc attgagcagc acgccccctt cgccgcgcac gccctcggtg
    11161 atcagcgtgc cgcggacgct gggcggccag accatgcccg tcgggtggaa ctgcacgaac
    11221 tccatgtcga tcaggtccgc cccggcccac agcgccatcg cgtgcccgtc ggccgtgtat
    11281 tcccaggaat tgctgttgat ctcccagcac tggccgatgc cgcctgtggc cagcaccaca
    11341 gccttcgccc gaaacaccac gaaccggccg ctgtcgcgct gatagcccat gcagccactg
    11401 gcgcggccgg cggtgagcag cacgcggcgg atcgtgcatt ccatgtgcac gtcgattccc
    11461 tgatgcactc cgcggtcctg gagcgtgcgg atcatctcca ggcccgtccg gtcgccgacg
    11521 tgggccagcc gcgggtaggt gtggccgccg aaatttcgct gcaggatgtc gccggtcttc
    11581 gtgcggtcga agaccgcgcc ccactcttcg agctcgcgga cccgctcggg tgcttcctgc
    11641 gcgtggagct gcgccatccg ccactggttg aggaactggc cgcctttcat cgtgtcgcgg
    11701 aaatggacct gccagttgtc gcgggtatcg acgttcgaca gggcggcggc gattccgccc
    11761 tcggccatga cggtgtgggc cttgccgagc agcgacttgc agaccagggc cgttcgcacg
    11821 ccacgggccg aagcctcgat cgcggccctc aggccagcgc ctcctgcacc gaccacgacc
    11881 acgtcgtatt cgtgcgtctg gaatttagac atgaatcaca ggatccgcag gtcggtgagg
    11941 cgccccgagg ccaccatcca gacgtagaaa tcggcaaagc ccacagagaa caggctcagc
    12001 caggcgaaga gcatgtggtg ttcgttgagg gcgctggccc aggaccaggc ccgctgccgg
    12061 acgttgccca gcgccacgca cgagaagcaa tcgacgttgc cgccgaccag gtgccgcagc
    12121 gaatggcacg agaacgtgta gagcgaaagc agcgtcacgt tcgccgccag gaccagcgtc
    12181 gccacgctga cgccaaacga atggcccgca gcagtgggcc aaatgcagga atgaatgacg
    12241 tcgatcgcca ggatgaccag aaagatcagc gcaaaataca ggaagtagcg gtgcagattc
    12301 tgaaacaaga acaggcccgt ctcgccccgg tagcctttgc cgcgggcctc gcccaccgcg
    12361 cacgccggtg gatcgaggaa gaacgcccgg taataggcct tgcggtagta gtagcaggtc
    12421 aggcggaatc cgcccggcgc ccacagaatc aaaaacgccg gcgacagcca ggccggcagc
    12481 cacggcagca caatcagcgg cgaatagaag ggcgagagca gataggcgtg attctccagc
    12541 acgccagtcc cggggccgac gtgatagcgg gcattcatca ccgcccgcgc ggtggcgtag
    12601 acgccgaacg cacccagggc cagtgccgtg gccagcggcc cggcccacca tgcatctcgg
    12661 cgctgggtta ctcccagtcc gcctcggtcc gccgctggag cgcccgccgc catttcactc
    12721 cccgaataag gttggcggca ctatatcgca gcggctcccg cggcgtcaaa tcgctaagcg
    12781 ggccccctgc ttcattgcac cctgcttttg caccacaatt cccgcatctg ggatccacac
    12841 aactttgagg tgaaccatga ctctcgctgc gcgatcctgt tggggggcta ccttgctggt
    12901 ggcaatgttg gcgtcgtgcg cctggggcca gggtaagaag ccggacgtcg cgctcgacgg
    12961 ggagctgtcg cggctgctcg tgcggcacgg ctttacaggg cggatcgagg agcagctcga
    13021 ggcgcggctg ggacggaagc tcgatccgca gctggcggat ctggggcgga acctgttctt
    13081 cgacccgatc atggccctcc gcggcgacaa ttcgtgcgcc gggtgccacg cgccgcagtt
    13141 cggcttcgcc gactcgcagt cgatcgcgat cggcatccgc aacaacaacg
//
LOCUS       DASRJB010000816         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1930518, whole genome shotgun sequence.
ACCESSION   DASRJB010000816 DASRJB010000000
VERSION     DASRJB010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930518"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFB80_21405"
     CDS             complement(<1..622)
                     /locus_tag="VFB80_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN36407.1"
                     /translation="MNAAGVTCRSVVKDYGSGGSLVRALRGVDLEIRPGELTLLVGPS
                     GCGKTTLISIVAGTLNPTGGDVQAMGAELGRLSKGQRTAFRARLVGFVFQQFNLLPSL
                     TAAENVAVPLVINGAARQPALARAREVLASVGLADKFNALPKELSGGQQQRVAIARAL
                     VHRPKLLVADEPTSAIDAHTGHAIMELIRAVAVQPDRVAIVVTHDPR"
     gene            complement(626..1765)
                     /locus_tag="VFB80_21410"
     CDS             complement(626..1765)
                     /locus_tag="VFB80_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN36408.1"
                     /translation="MTWIALKMLTGDRGKYLAIILGVAFASLLITEQAAIFCGIMLRT
                     TSQIRDTHGEDIWVMNPNVRYLDDAKAISDNDVFRVRGVPGVAWAVNLYRGQGQAQLA
                     SGNYQGVILLGIDDASLTGAPTQMLLGQVGDLQIPDAILVDEAGFHQMWPGEPLSIGK
                     TIEMNDRRAVVVGVYRASQTFMTMPVIYTRFSQGTLFVAPTRRLMPYVLAKCRPGFSP
                     PAVARQITERTGLKALTSDGFTRLTMLYYLRHTGIPINFGTTVLLGFVVGCAIAGQTF
                     YLFTIENLKQFGALKAMGLSDRRIVEMILVQALVVGAIGYALGVGLATIYGIVAQMYV
                     PLLAFYLPWQVLAISAVAVLLIALASSLLSIRRVLVLEPAVVFQG"
     gene            complement(1897..2322)
                     /locus_tag="VFB80_21415"
     CDS             complement(1897..2322)
                     /locus_tag="VFB80_21415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HZN36409.1"
                     /translation="MLRWKTILFAGLLTSGLIGAAAADDKQQWQGTWTMVSCIANGES
                     TEGNVQWVVSGDAYKIRIDGKLGSDRYPFQLDASKKRIDVNHHDTPPGTWGGKLKGIY
                     EIKGDTLKVCYDLKGQRYPTSFAAGPGSGQVIYQFRRAR"
     gene            complement(2491..>2951)
                     /locus_tag="VFB80_21420"
     CDS             complement(2491..>2951)
                     /locus_tag="VFB80_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36410.1"
                     /translation="SNPEWMDQDRIIQTWCRFYATADDQQRESLRQIGSHTAYVVFAH
                     RSAVRALRTGNPDWLELALLALSLNDLRDSFSRGLDYHMGSRSGATFRMLEIVARVAG
                     AIGTDFPNMLDRVKAISSPYTAEVLSSKLDYPDRSIQFYEDPNGKMIGWP"
BASE COUNT          532 a          952 c          959 g          508 t
ORIGIN      
        1 cgcggggatc gtgcgtgacc acgatcgcca cgcggtcggg ctggaccgcg acggcgcgga
       61 tgagctccat aatcgcgtgg ccggtgtgag cgtcgatggc ggaagtcggc tcgtcggcga
      121 cgagcaactt gggccgatgc accagggcgc gggcaatcgc cacgcgctgc tgctggccgc
      181 cggagagttc cttgggcagc gcattgaatt tgtcggccag gcctaccgaa gccaggacct
      241 cgcgggcccg cgccagcgcc ggctgccgcg ccgccccgtt gattaccagc ggcacggcga
      301 cgttctcggc cgcggtcagc gaaggcagca ggttgaattg ctgaaagacg aagccgacca
      361 gccgggcgcg aaacgcggtc cgctggccct ttgagagccg acccagttcg gcgcccatcg
      421 cttgcacgtc gccgccggtc ggattgagcg tgccggcgac gatcgaaatc agcgtcgtct
      481 tgccgcagcc gctggggccg acgagcagcg tcagctcgcc gggacgaatc tccaggtcca
      541 cgccccgcag ggcccgcacc agcgagccgc cgctgccgta atccttgacc acgcttcggc
      601 aagtgacgcc cgcggcgttc atggttcacc cctgaaagac gacggcgggc tccagcacca
      661 gcacgcggcg gatgctgagc aggctgctgg ccagcgcgat cagcagcacg gcgaccgcgg
      721 aaatcgcgag cacctgccat gggaggtaaa acgccagcag cggcacgtac atctgggcga
      781 cgatgccgta gatcgtcgcc agccccacgc cgagggcata gccgatcgca ccgacgacca
      841 gggcctggac caggatcatc tcgacgatcc ggcggtcgga caggcccatc gctttcagtg
      901 cgccgaattg cttgaggttc tcgatcgtga acaggtagaa tgtctgcccc gcgatggcgc
      961 agcccacgac gaacccgagc agcaccgtgg tgccgaagtt gatcgggatg ccggtgtgcc
     1021 ggaggtaata gagcatcgtc agccgggtga agccgtcgct ggtcagggcc ttcaggccgg
     1081 ttcgctcggt gatctgccgg gccaccgcgg gcggcgaaaa gccaggccgg catttggcga
     1141 gcacgtaggg catcaggcgg cgggtgggag cgacgaacag cgtgccctgg ctgaagcggg
     1201 tgtagatgac cggcatcgtc atgaacgtct gcgaggcgcg atagacgccg actacgaccg
     1261 cgcggcggtc gttcatctcg atcgtcttgc cgatcgagag cggctcgccg ggccacatct
     1321 gatgaaagcc ggcttcatcg acgagaatcg catcggggat ttgcaggtcg cccacctggc
     1381 ccagcagcat ctgggtcggt gcgccggtca gactggcgtc gtcgatgccc agcaggatca
     1441 cgccctgata gttgccgctg gcgagttggg cctgcccctg gccgcggtag aggttgaccg
     1501 cccaggcgac gcccggcacg ccgcggacgc ggaacacgtc gttgtccgag atggctttgg
     1561 cgtcgtcgag gtagcggacg ttcgggttca tcacccaaat gtcctcgccg tgcgtgtcgc
     1621 ggatctggct cgtcgtgcgg agcatgatgc cgcagaagat cgcggcctgc tcggtgatca
     1681 ggagcgacgc gaacgccacg cccaggatga tcgccaggta tttgccgcga tcgcccgtca
     1741 gcatcttgag cgcgatccag gtcatggcgg cgactcctcc gcgttccagg gccatccctg
     1801 gatgccaaca acccgcgccg ctcgtccgcc gcctcaggca gcggccatga tgaatccttc
     1861 ggacgctcgc tggcgaagtc aagccgacgc cgaggcctag cgcgcccggc gaaactgata
     1921 gatcacctgc ccgctgccgg ggccggcggc aaagctggtg ggatagcgct ggcccttcag
     1981 gtcgtagcag accttgagcg tgtccccttt gatctcataa atgcccttga gcttgccgcc
     2041 ccacgttccc ggcggcgtgt cgtggtggtt cacatcgatc cgcttcttgc ttgcgtcgag
     2101 ttgaaacgga tagcggtccg accccaactt gccgtcgatc cgaatcttgt aggcgtcgcc
     2161 gctcaccacc cactgcacat tcccctccgt cgattcccca ttggcgatgc acgacaccat
     2221 cgtccaggtc ccttgccact gctgcttgtc gtcggccgct gccgccccga tcagcccact
     2281 ggtcagcaaa ccggcaaaca gaatggtctt ccagcgcagc atggcaattc tcccaatgcg
     2341 attggaaacg cggctactgc tatctacgcc gtagccgtgt cgctgccacc ggaattcatg
     2401 cgtgcggcat cgcttatgcc gattgcactg ccgaggaggc ggagccggat gccgaatgga
     2461 aatgcagtgc gtggcagccc tcgccaatgt tcacggccag ccgatcatct tgccgttggg
     2521 atcttcgtag aactgaatcg atcgatctgg ataatcgagt ttgctcgaca ggacttccgc
     2581 ggtatatggc gacgagatgg ctttgacgcg gtcgagcatg tttggaaagt cggtgccaat
     2641 ggcgcccgcg acccgcgcca caatttccaa catgcggaat gtagcgccgg accgagatcc
     2701 catgtggtaa tccagtccgc gcgaaaacga atctctcagg tcattcagcg acagggccag
     2761 cagcgccagt tccagccaat cgggattccc agtgcggagc gcccggaccg cggagcggtg
     2821 ggcaaacaca acgtaagccg tgtgggaacc aatttgccga agcgattcgc gctgctggtc
     2881 atcggcagtc gcgtagaacc gacaccatgt ctgaatgatg cggtcttgat ccatccattc
     2941 gggattgctg a
//
LOCUS       DASRJB010000817        16640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2208593, whole genome shotgun sequence.
ACCESSION   DASRJB010000817 DASRJB010000000
VERSION     DASRJB010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208593"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFB80_21425"
     CDS             <1..937
                     /locus_tag="VFB80_21425"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZN36411.1"
                     /translation="LMAGLPVAACGATINRLCGSSLQALNQASHAIASGFEDVQIVGG
                     LEHMQHLPMDHGLDINPKLFQRTSKGALMMGVTAEFLAQTQDISRADQDAFALRSHQR
                     AAAAHAAGQFKREIVPLYARDEEGQRFLATGDQCVRPDTTLEALAALEPAFMPKVGTV
                     TAGNSSPLNDGAAALLIMSEGKAKSLGLQPLVRVRATAVAGVEPSVMGTGPVPAVKKV
                     LQRAGLTLKDIDLIELNEAFAAQALACIRMLGLEEDKVNVRGGAIAIGHPLGASGARI
                     STTLINAMVDRGAKLGLATMCIGLGQGIATVFERI"
     gene            947..2029
                     /locus_tag="VFB80_21430"
     CDS             947..2029
                     /locus_tag="VFB80_21430"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN36412.1"
                     /translation="MSNPNQFFPQSGGGFPQQGGGFYGQPPKKSGGSTIWIILGVIGA
                     GVVLLCGACCGGVMLLSRPPVASAAAKQPFTFADVPPPPFPDRGNAEEVEPGVRLDEI
                     MIKDEDGGFYETPGHGGWLHLYLPAGEHQPKSLPCVLIAPAGSTLLAGMDLGDGDQPE
                     HVPYVKAGCAVLAYELDGPCLDDTDLDEQKDAFRAFKDSRAGLVNGRNALEYLLAKVP
                     EVDPARIYAAGHSSAATHALLFAAHEPRLAGVMAYAPAPDLPKRMGPQLAVFRFIMPG
                     VVDFVTQSSPRTHVGSIKCPTFLFHAQDDSNCPIADTNAFADELKQKGTDVKYVTAAS
                     GDHYDSMINEGIPAGIQWLKERGAIK"
     gene            2236..3354
                     /locus_tag="VFB80_21435"
     CDS             2236..3354
                     /locus_tag="VFB80_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HZN36413.1"
                     /translation="MNDPRYAIADTSQILSPGLVVFRDLVEQNLAEMIRIAGSPARLR
                     PHCKTHKMREVTELQLARGITKHKCATIAEAELLAEAGARDIFLAYNPVGPNIGRVAA
                     LVRKYPEANFKVTADHPRPLAALGEALSSAGCSVEVLLDIDCGQHRTGIPAGAEARRL
                     YEQIAATKGIRPGGLHLYDGHNHQTDLAERRAAVHGCCRPALALYDELTARGLGVPRI
                     VAGGTGSFPIFAEIDHPAIELSPGTVIFYDFGYGDMFPDLRFTPAALLLTRVISRPTA
                     ERVTLDLGYKALASEMPLANRLRFPDLPDAQPVLQNEEHLVLETSESRRFTPGDELLA
                     IPKHICPTCNLQQQVFVVSGGRHVGNWLVRGRDRVLSV"
     gene            complement(4147..5424)
                     /locus_tag="VFB80_21440"
     CDS             complement(4147..5424)
                     /locus_tag="VFB80_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZN36414.1"
                     /translation="MRYRRLSLALSLLLSFLPFAASAADFPPPEKLPANPQLPDPLVM
                     LDGTKVTTKEQWEQKRKPELKELFQHYMYGRLPPEPRVQKANLLFTDPNALGGKATLS
                     EVKITFEQPMLEHAIHVLLIRPNGVKQPPVFLGMNFCGNHTLLDHPKIHLPEGWVRNS
                     CPGAVNERATDKGRGGQQDVWNADLIVSRGYALASFYSGDIDPDTADLSDGIGPAYWK
                     DGKPAGDDAGTIALWAWGFHRAVDYLDRVEGFQIDPKRIAVVGHSRNGKTALLAGAFD
                     ERIAMVIPLQAGCGGTAPSRTADTRAESVKRINTSFPHWFCDNFPLFNDQVERLPFDQ
                     HCLIALCAPRPVLLPNAEEDLWANPSGQFELLQAASPVYRLLGAEGVPASAKPEMNKL
                     LDSRLGYFIRPGKHAMTRADWEVFLAYADKYFK"
     gene            5551..6876
                     /locus_tag="VFB80_21445"
     CDS             5551..6876
                     /locus_tag="VFB80_21445"
                     /inference="COORDINATES: protein motif:HMM:NF041199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HZN36415.1"
                     /translation="MNRAWIAFAVVLLAAGVADAQTREEKVRNDKAKVEAAGFWIYNN
                     LAKGIADAKKTGQPLLVALRCIPCEDCVKLDEELVETDPKLRPLLEKFVRVRIVSANG
                     LDLSLFQFDTDQSFAVFMLNADGAIYGRYGTRSDHSHWADDVSIEGLAKALDGALALH
                     AAYPANKAELALKRGPAPEFAVPENLPRLKGKYGSQLNYEGNVVQSCIHCHQIGDSLK
                     QMHRDKGGALPEDVLFPYPHPKALGLILNPRERATVLRVESGSLAEQAGFRAGDEIAK
                     IAGQTPLSIADVQWVLHHVPAAGGSVVATVQRGGKAADVKLELPAGWRRRDDIAWRAS
                     SWELRRMALGGLFLKSISPELRAEQKLPASGLALKAEHVGQYSPHDGAKRAGFQAGDV
                     LISFDGRTDLTRETDLLHHALNVVKPGNQIPVVVLRNGQKHNFTLTTGM"
     gene            complement(6878..9121)
                     /locus_tag="VFB80_21450"
     CDS             complement(6878..9121)
                     /locus_tag="VFB80_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36416.1"
                     /translation="MRGLKHGERSLVLAAAAWLLAAASVSRADDADQRASAADAAALR
                     RVVSLWHSGQQQPAWFLYQDTVRRLNADLKLNEPRQARLSALRREAAEAIGAQFGPRP
                     IQLDRKSRLVREGDWPQWGGWGGRNNVALADAGPEDWNPGDFDRRTGVWLPGTSRNVK
                     WTAALGSQTYGSPTIAGGKVFVGTNNATGYLKRAPADVDLGCLLAFRERDGHFLWQHS
                     SEKLPAGRVHDWPMQGICSTPLVEGDRLWFVTNRCEVRCLDTEGFYDGVDDGLPAAAE
                     PVRLFDLPRPRPPLLDTLPELAAALDAGRLPEELRPRFTAAGIELPADVAVKQADLPG
                     AAERRWLLEANFGHAQRGLLIGYDRRQLSAFQILTVEDRSEADVVWVLDMLKELGVSP
                     HNMTVCSPTAYGDVLFICTANGVDESHSTIPAPQAPTFIALDKRTGQVLWTDNSASSQ
                     VHHANWGSPAVGVFAGVPQVIFGGGDGWVYSFHATEWQGGKPTLLWKFDTNPKDAVLT
                     LGGRGMRNEPIAFPVVYDGMVYVTIGQDPEHGEGDGGLWCLDPTRRGDVSPQLAVRAD
                     DRTQIVPYRRKQAIDAAAGEIAIDNPNSAALWHYGRRDANGDGKLEFEEAIHRSISSI
                     VAQDDLLLAVDFSGLSHCLHAKTGRLYWTCDLLAAVWSTPLVVGDRVYVADEDGDVAI
                     LRLHPDPKLGTQWKPDDKPPFFGTREPLFEINMGRSVYCTPAFANGVLYIAAKDHLFA
                     IPGPVSP"
     gene            9196..10428
                     /locus_tag="VFB80_21455"
     CDS             9196..10428
                     /locus_tag="VFB80_21455"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZN36417.1"
                     /translation="MSRLMTVVSLSLLCGFSGQAAAQNRTQLEQARHKLVDEILVPAG
                     VTNPRVVQAMRDTPRHEFVSRDQIAKAYLDMSLPIGGQQTISSPLIVSQMTQALDPQP
                     GDKVLEIGTGSGYQAAVLSPLVKEVYTIEIVESLGKSAEARLKRLGYKNVHVKVGDGY
                     QGWPQHAPFDKIIVTCSPEKAPQPLVDQLADGGLMIVPVGERYSQTLYMLRKKGGKLE
                     SLALLPTLFVPMTGKAEEQRAVQPDPTNPQVVNGSFEQDASQFKNGAQPGWYYERLVE
                     WKTDAKAPEGEHFVEFKNSEPGLGSHLLQGFAIDGRKVSQLTVSGQVRTENVVGGADD
                     AAPHIVITLYSEQRKDLGNLLLGPFKGTSDWHQESKTFRIPPEAREGILRIGLFGATG
                     VAAFDKVEMRKTPTGTPQ"
     gene            10576..13464
                     /locus_tag="VFB80_21460"
     CDS             10576..13464
                     /locus_tag="VFB80_21460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN36418.1"
                     /translation="MLRRLLAIAAILVASLAGCNLAGPNRAYDAELAHYKQVAATVDH
                     PELTTPPSDNINTPEPRTLDHNAAPQEPREWWDMTVEEALQTALARTSVLRDLGGTVI
                     RSPDTTRTILGPSLIATEPRFGMEAALSEFDATLASSVIAEKNDRMINNFVASGGTRF
                     FKQDLINFQSQISKLGVAGTRMTLRGTSTYDFNNASFNRFPSVWDTLVEAELRQPLYQ
                     GAGARFNRLAGPNAIPGFYNGVLIARVNTDMSVAEFEIGIRDVVSNVENAYWDLYFAY
                     RDLDARLQARNVALEAWRRVKVIADTEQQEGALEREAQLREQYFRYEEDVQNALAGTL
                     QSSTTNNNGSSGGSFRGLGGVLVSERRLRLAIGLPITDGRMIRPADEPTAARVTFDWP
                     SSSGDAIARRPELLRQRLAVKRREMEFVAARNFLLPRFDLLGRYRVRGLGKALIGDQV
                     LPVDPADPEGLFENSSLKNLALGNFQEWQLGGEVSLPIGFRRGHAAVRNAQLFVAREK
                     AVLEEQERQVIHDLSNALGDVDRTYKIMQTAYNRRLATVDNVNIVRTRIETGQNVTPE
                     QLLESGRRLAEADIQYHKALVEHMLAIKNVHFEKGTLLEYGHIYLAEQVSIVNARPPP
                     ADVQQPTPAQPAEGIPPPPVEVMPAPQPAPPPQPMPPPPPATGAAPAGTGPAPIGTGA
                     APRLPLPLGEGWGEGLDALPVQGGLPPAPAQPQSPPIVVQPPQAPPVEGLPPAPSQSS
                     GPALAPPAAETSSAPSGLPTLPSTGRAPILQWSRRRQPEQPANVNVPANPFMLEVRPQ
                     PELGLPPANQAPSPPPLPPPSRQVAPAPSPPPQAPRPSAAFIERLWYSHEPATTRLPN
                     VDVVVPVQAPVAPPTPAAWQPPVMDQPVPTTRLPQPEVEPPPVLVQPAAPVPAPAMRL
                     PEPAPEPPPVRLPLPPAYPPIDPAQRLPQPAPEARAENEIQRLPLPYP"
     gene            13515..15503
                     /locus_tag="VFB80_21465"
     CDS             13515..15503
                     /locus_tag="VFB80_21465"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN36419.1"
                     /translation="MNIGSPPPLDDFAPAEAWQEIESALEELAQLSRRDLPPGEFHSR
                     LMERMTGVLAAVGGVVWSFGSEGRPAIECQLHLDQSLAGNAAELGRHQRLAESVAAAG
                     EARLVPPAFRDAQHANASPWLAILCPISPDGEARYVVEVFQRPDGRSAVEEGYLRLVR
                     TACDIAEQFHRGRLLRELKANQGELASLVEYSALIHQRLDLRLTAAAIANEARRVLSC
                     DRVSVLACAGRHPKVAAISGVETFDRRSGIVRALEAVARPVVAVGEPLCYPADADELP
                     EPVAEQVQTLVDQSHARGLLVIPLLNGPADAARTIGLLAIEQFGQEIDSGLRKRAAAL
                     VETSAAALSQAIEYERIPLRGPLTWLAAVLGLGPGRRWSPALTAIAVLAAAVIALAVI
                     PVELTVEARGRLMPVRRQHVFAPSDAVVVELTQQGGAEVQLGQTLAKLHSPALDIAQS
                     ELVGKQRTVQEELLAAETEFLRGETEGKSPASRTQLAGRVQQLKEELRGLDAQLQIVR
                     QQLKELEITSPLAGVVITWDAQRQLAGRPVKRGDSLLTVADLSGPWELLLDVPDDRAG
                     PILETSGKPEEMQVTFQLGTDPGVVREAKVKEVSPATELSPENAPSVLVTAALPQTEK
                     LTLRPGASVVARVHCGRRSLGYVWLHEFWEAVRLRLFL"
     gene            15631..16455
                     /locus_tag="VFB80_21470"
     CDS             15631..16455
                     /locus_tag="VFB80_21470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN36420.1"
                     /translation="MCFSQACGAQEIKIAGVLVKLIDQLDVPAREQGTIAQLAVQEGS
                     RVKADDLLMRIDDTEARYAEDRARVEMAVAAQQAASDVAERLAERTLATAEGELRRAD
                     EARAKLRNIVTEAEYEKLRLAVDQAKLAVEKAQQDRSVAALQRDLKKVELEFAARQTA
                     RHQATAPFAGVIVQVHKRQGDWVEPGDKMLRLIRLDRLRVEGFLDAAQAKSSLEGRPV
                     ALTLDAPASPGTAFTGKLIFVSPEIDPINRQVRVLAEIENPTFALQPGSRGTLTIR"
     gene            16461..>16640
                     /locus_tag="VFB80_21475"
     CDS             16461..>16640
                     /locus_tag="VFB80_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36421.1"
                     /translation="MLASATVDRPLLLRQRRDLVVREQWFAGQRHFVLHDPLALTYAY
                     LTEIEHAVWALLDGRT"
BASE COUNT         2857 a         5593 c         5557 g         2633 t
ORIGIN      
        1 tctgatggct ggcctgcccg tggctgcctg cggggcgacg atcaaccgtc tttgcggcag
       61 cagcctgcag gccctcaacc aggcgtcgca cgcgattgcc tccggcttcg aggacgtgca
      121 gatcgtcggc ggcctggagc acatgcagca cctgccgatg gaccacggcc tggacatcaa
      181 ccccaagctc tttcagcgga cgagcaaggg cgcgctcatg atgggcgtaa ctgccgagtt
      241 cctggcccag acgcaggaca tctcgcgggc cgaccaggac gcctttgccc tccgcagcca
      301 ccagcgggcc gcggcggctc atgcggccgg ccaattcaag cgcgaaatcg tgccgctgta
      361 tgcccgcgac gaggaaggcc agcggttcct ggccaccggc gaccagtgcg tgcggccgga
      421 cacaacgctg gaggccctgg cggcgctcga accggcgttc atgcccaaag tcggcaccgt
      481 caccgccggc aacagctcgc cgctcaacga cggcgcggcg gcgctcttga tcatgtcgga
      541 aggcaaagcg aagtcgctcg gcctccagcc gctcgtccgc gtgcgggcca cggcggtcgc
      601 cggcgtggag cccagcgtga tgggcaccgg cccggttccc gcagtgaaga aggttctcca
      661 gcgggccggc ctgacgctga aggacatcga cctgatcgaa ctgaacgagg ccttcgccgc
      721 gcaggcgctg gcctgcatcc gcatgctggg cctggaggag gacaaggtca acgtccgcgg
      781 cggcgcgatc gccatcggcc acccgctggg cgccagcggc gcgcggatct cgacgacgct
      841 gatcaacgcc atggtcgatc gcggcgcgaa gctcggcctg gcgacgatgt gcatcggcct
      901 gggccaggga attgccacgg tttttgaacg tatttgaggg agcaatatgt cgaaccccaa
      961 ccagttcttt ccccagtcgg gcggcggatt tccacagcag ggcggcggct tctatggcca
     1021 gccgccaaag aaaagcggcg gctcgacgat ttggatcatc ctcggcgtaa tcggcgcggg
     1081 ggtcgtgctg ctctgtggtg cgtgctgcgg cggcgtgatg ttgctcagcc ggccgccggt
     1141 cgcttcggcc gccgccaagc agcctttcac gttcgccgac gtgcccccac ctccgtttcc
     1201 ggatcgcggc aatgcggaag aggtggagcc cggcgtccgg ctggacgaga tcatgatcaa
     1261 ggacgaagac gggggctttt atgaaacgcc cgggcacggt ggctggctcc atctctactt
     1321 gccggcgggg gagcatcagc caaagtcgct gccctgcgtc ctcatcgccc cggcgggcag
     1381 cacgctgctg gccggcatgg atctgggcga cggcgatcag ccggagcacg tgccctacgt
     1441 gaaagcgggc tgcgcggtgc tggcctacga actcgacggc ccctgcttgg acgacaccga
     1501 tctcgacgaa caaaaggacg cctttcgcgc gttcaaggat tcgcgggccg gtctggtcaa
     1561 cggccgcaac gccctggagt acctgctggc caaggtgccc gaggtcgatc cggcccgcat
     1621 ctatgccgcc gggcacagct cggccgcgac gcacgcgctg ctcttcgccg cgcacgagcc
     1681 gcggctggcg ggcgtgatgg cctatgctcc cgcgcccgac ctgccgaagc ggatgggccc
     1741 gcagctcgcc gtgttccggt tcatcatgcc gggcgtggtc gattttgtga cgcagagctc
     1801 gccccgcacg cacgtcggca gcatcaagtg ccccacgttc ctgttccacg cccaggacga
     1861 cagcaactgc ccgatcgccg acaccaatgc cttcgccgac gagctcaagc agaaggggac
     1921 ggacgtcaaa tacgtcaccg cggccagcgg cgaccattac gactcgatga tcaacgaagg
     1981 catccccgcc ggcattcagt ggctgaagga acgcggggcg atcaagtgaa tggagtcgcc
     2041 agggaaggct cggcgtgctc atcggaagaa tctcacacag aggcacagag gacacagagc
     2101 agtcagttgg gttcagaaaa ccgggtctcc catcaattcc tttcctcctc tgtggtctct
     2161 gtggctccgt gtgagaattc ctatctgcca tccgttccaa gcgcggttct cctcccaaag
     2221 atagctgaac gaatcgtgaa cgatcctcgg tacgccatcg ccgacacttc gcagattctc
     2281 tcgccgggtc tcgtcgtgtt ccgcgacctg gtcgaacaga atctggccga gatgatccgc
     2341 atcgccggct cgcccgctcg gctccgcccg cactgcaaga cgcacaagat gcgggaagtc
     2401 accgagctcc agttggcccg cggcatcacg aagcacaagt gcgcgacgat cgccgaagcg
     2461 gagctgctgg ccgaggccgg tgcgcgggac atcttcctgg cctataaccc cgtcggcccg
     2521 aacatcggcc gcgtggcggc gctggtgcgg aagtacccgg aagcgaactt caaggtgacg
     2581 gccgaccatc cgcggccgct cgccgcgctg ggcgaggcgc tttcgtccgc cggatgcagc
     2641 gtggaggtgc tgctcgacat cgactgcggc cagcaccgta cgggaatccc cgccggagcg
     2701 gaagcccgcc ggctctatga gcaaatcgcc gcgaccaagg gaattcgtcc gggcggcttg
     2761 cacctctacg acggccacaa ccatcaaacc gacctggccg agcggcgggc ggcggtccac
     2821 ggctgctgtc ggccagcgct ggccttgtac gacgaattga ccgcgcgggg gctgggcgta
     2881 ccgcggatcg tggccggcgg cacgggctcg tttccgatct tcgccgagat cgatcatccg
     2941 gcgatcgagc tcagcccggg cacggtcatt ttttacgact tcggctatgg cgacatgttc
     3001 cccgacttgc ggttcacgcc cgcggcgctt ttgctgactc gcgtcatcag ccggccgacg
     3061 gccgagcggg tcacgctcga cctgggatac aaggctctgg cctccgagat gccgctggcc
     3121 aatcggctgc ggttccccga cctgcccgac gcccagccgg tgctgcaaaa cgaagagcac
     3181 ctcgtgctgg aaacgagcga gtcccgccgc ttcacgcccg gcgacgagct gctcgcgatt
     3241 cccaagcaca tctgccccac ctgcaacctc cagcagcagg tgtttgtcgt ctccggcggc
     3301 cggcacgtag gcaactggct cgtccgcggc cgcgaccgcg tgctgagcgt ttagccgggc
     3361 cttcgacttg cgcggccccc tggatggatg gcacgcaact gacgcctttc gcgattttcc
     3421 gccacaactg ctgacgcgtc atcagcttgc ggcgacaagg ccaaaatcgg ttaagtagta
     3481 cttaataagt gctagagagc agatattagc cggagcagcc cttgctccat gatgctccgc
     3541 gcgacgcgag ctccgccaaa gccgcgacca gtggtcgcgc tctgccagtg cagcgcgacc
     3601 acgccgctgc cggcgactgg cggagcgagt tgacttaacg aacccggaat cgacaaagaa
     3661 cttggcaccg ccgcttcggc agcagccgct tgggaaaaca cgagccgggc gtgttgatcg
     3721 cctcttctcg acaagtggac agattatacc atagccttcc ccgattttgg ccgcttcgcc
     3781 cgcaaaatgg ccgaacaacg ccttcgcaaa tgcttacgcc gtttggcctt gcaactattt
     3841 tcaagaaaat gcgcgccgca gagggctttc tccagatggc aagtggcaga ttggacggcg
     3901 accacgcgga ttgcaggccc aacgggacga ccttcgtcag ccgcgggcgt agcccgcggt
     3961 aatcgcacca caaggagcgc gcaggcccaa cgggccggaa ttgaggcggt acggattcgt
     4021 ccaatctcgc ccttgcagga ctggcggatt ccctttcgct cccaccctgg gctcgcggct
     4081 gcgccgcagc gcccagggct gacgaaggtc ggcccattgg gcctgcattc gccagcggag
     4141 ccccggctat ttgaaatatt tgtcggcgta ggccaggaac acttcccagt ccgcccgcgt
     4201 catggcgtgc ttgccggggc ggatgaaata gcccaagcgc gaatcgagca gcttgttcat
     4261 ttcgggcttc gcgctggccg gcacgccttc cgcgccgagc agccgataga cgggcgacgc
     4321 cgcttgcagc agctcgaatt gcccgctggg gttggcccac aggtcctcct cggcgttggg
     4381 caagagcacg ggccgcgggg cgcagagggc gatcaggcaa tgctggtcga agggcagccg
     4441 ctcgacctgg tcgttgaaca gcggaaagtt gtcgcagaac cagtgcggga agctggtgtt
     4501 gatccgcttg accgattcgg cccgcgtgtc ggccgtgcgg ctgggcgcgg tgccgccgca
     4561 gccggcctgc agcggaatca ccattgcgat ccgctcgtcg aaggccccgg ccaacagcgc
     4621 cgtcttgccg ttgcgggagt ggccgacgac ggcgatccgc ttgggatcga tctgaaaacc
     4681 ctcgacccta tccagatagt ccacggcgcg gtgaaatccc caggcccaga gggcgatcgt
     4741 gcccgcgtcg tcgccggccg gcttgccatc cttccaatac gccgggccga tcccgtcgga
     4801 gaggtcggcc gtgtcgggat cgatgtcgcc gctgtagaag gaggccaggg catatccgcg
     4861 gctgacgatc aaatcggcgt tccagacgtc ctgctggccg ccgcggccct tgtcggtcgc
     4921 ccgctcgttc actgcgcccg ggcaggagtt gcggacccaa ccttccggca ggtgaatctt
     4981 gggatgatcg agcagcgtgt ggttgccgca aaaattcatt cccaggaaca ccggtggctg
     5041 cttgacgccg ttgggccgga tcagcagcac gtgaatggca tgctccagca tcggctgctc
     5101 gaaggtaatt ttcacttcgc tgagcgtcgc cttgccgccg agagcattgg gatcggtgaa
     5161 cagcagattg gctttctgca cccgcggctc cggcggcagc cggccataca tgtagtgctg
     5221 aaagagctcc ttcagctccg gcttgcgttt ttgctcccac tgctccttgg tcgtgacctt
     5281 cgtgccgtcg agcatcacca gcggatcggg cagctgcgga ttggccggca gtttttccgg
     5341 aggcggaaag tcggcggcgc tggcggcgaa tgggaggaag gagagaagga gagagagagc
     5401 gagggagaga cggcgatagc gcatgatgat tggctccgga tcggggatgc cgggagtgta
     5461 ccgcagctgc tgtgcgaatc ctactcgccg gttgagttgc ctggcgggca aggcaataat
     5521 cgggcctcca agttcaccaa ggaccagctc atgaaccgcg cctggattgc ttttgccgtc
     5581 gtgctgctcg ctgccggcgt cgccgacgct cagactcgcg aggaaaaggt ccgcaacgac
     5641 aaggccaagg tcgaagccgc tggcttctgg atttacaaca acctcgcgaa gggaattgcc
     5701 gacgcgaaga aaactggtca gccgctgctg gtggcactgc ggtgcattcc gtgcgaggac
     5761 tgcgtgaagc tggatgaaga gctggtcgag accgacccca agctgcggcc gctcttggag
     5821 aagttcgtcc gcgtgcggat cgtttcggcc aacggcctcg atctgtcgct atttcagttc
     5881 gacaccgacc agtcgttcgc cgtgttcatg ctgaacgccg acggggcgat ctatggccgc
     5941 tacggcacgc gctccgacca ctcgcactgg gccgacgacg tgtcgatcga ggggctggcc
     6001 aaggcgctcg acggggcgct ggccttgcac gccgcgtatc cggcgaacaa ggccgagctg
     6061 gcgctgaagc gcgggccggc cccggaattt gccgtgcccg agaatctgcc gcggctaaag
     6121 gggaagtacg gctcgcagct caactatgag ggcaacgtcg tgcagagctg catccactgc
     6181 caccagatcg gcgactcgct gaagcagatg caccgcgaca agggaggcgc gctgcccgag
     6241 gacgtgctct ttccctatcc gcatccgaag gcgctcggcc tgatcctcaa tccccgcgag
     6301 cgggcgaccg tgctgcgcgt cgaatccggc tcgctggccg agcaggccgg tttccgcgcg
     6361 ggggatgaga ttgcaaaaat cgccggccag acgccccttt cgatcgccga cgtgcaatgg
     6421 gtgctgcacc acgtgcccgc cgctggcggc tcggtcgtgg ccaccgttca gcgcggcggc
     6481 aaggcggccg acgtgaagct cgaactgccc gccggctggc ggcggcgcga cgacattgcc
     6541 tggcgggcct ccagctggga gctgcggcga atggccctcg gcgggctgtt tctgaagagc
     6601 atctcgcccg agctgcgggc cgagcaaaag ctgcccgcca gcgggctggc tctcaaggcc
     6661 gagcacgtcg ggcagtattc gccgcacgac ggcgcaaagc gggccggctt ccaggcgggc
     6721 gacgtcctga tttcgttcga cggccgcacc gacctgaccc gcgagaccga cctgctgcac
     6781 cacgcgctca acgtcgtcaa acccggcaac cagattccgg tcgtcgtgct ccgcaacggc
     6841 cagaagcaca acttcacgct gacgaccggg atgtgaacta cggcgaaacc ggccccggaa
     6901 tcgcaaacag gtggtccttg gccgcgatgt agagcacgcc gttggcaaac gccggcgtgc
     6961 aatacaccga gcggcccatg ttgatctcga agagcggctc gcgcgtccca aagaagggcg
     7021 gcttgtcgtc cggtttccac tgcgtgccga gcttcgggtc gggatgcaga cgcaggatag
     7081 ctacatcgcc gtcttcgtcg gcgacataga cccggtcgcc gacgaccagc ggcgtgctcc
     7141 agaccgcggc tagcaggtcg cacgtccaat agagccgccc ggtcttggcg tgcaggcagt
     7201 gcgacaggcc ggagaaatcg acagccagca gcaggtcgtc ctgggcgacg atactggaga
     7261 tcgagcggtg aatggcctct tcgaattcga gcttgccgtc gccgttggcg tcgcgacggc
     7321 cgtaatgcca aagggcagcc gagttcggat tgtcgatggc aatctcgccc gccgcggcgt
     7381 cgatcgcctg tttccgccgg tagggcacga tttgggtccg gtcgtcggcc cgaacggcga
     7441 gctgcgggct gacgtcgccg cggcgcgtcg gatcgaggca ccacagtccg ccgtctccct
     7501 cgccgtgctc cggatcctgt ccgatggtga cgtacaccat tccgtcgtaa acgaccggaa
     7561 acgcgatcgg ctcgttccgc atgccgcggc cgccgagcgt cagcaccgcg tccttcgggt
     7621 tggtgtcgaa tttccagagc agggtcggct tgccgccctg ccactcggtc gcgtgaaagc
     7681 tgtagaccca gccgtcgccg ccgccgaaga tcacctgcgg cacgccggcg aagacgccga
     7741 cggccggcga gccccagttg gcatggtgca cctggctgct ggcggaattg tcggtccaga
     7801 gcacctggcc cgtccgcttg tcgagggcga tgaacgtcgg cgcctgcggc gcggggatcg
     7861 tgctatgcga ttcatccacg ccgttggccg tgcagatgaa gagcacgtcg ccatacgccg
     7921 tcggcgaaca gaccgtcatg ttgtgcggcg acacgcccag ctccttcagc atgtccagca
     7981 cccacaccac gtcggcctcg ctgcgatcct ccacggtgag aatctgaaat gccgagagct
     8041 ggcggcgatc gtagccgatc aggaggcctc gctgggcatg tccgaagttg gcttccagca
     8101 accagcggcg ctcggccgcg ccgggcaagt ccgcctgctt gacggccacg tcggcgggaa
     8161 gttcgattcc agccgcagta aacctcgggc gcagttcctc cggcaaccgg ccggcgtcga
     8221 gcgccgccgc gagctccggc agggtgtcga gcagcggcgg ccgcggtcgc ggcagatcga
     8281 acagccgcac cggctccgcg gcagccggca gtccgtcatc gacgccgtcg tagaagccct
     8341 ccgtgtcgag gcagcggacc tcgcagcggt tggtcacgaa ccacaggcga tcgccttcga
     8401 ccagcggcgt cgaacaaatg ccctgcatcg gccagtcgtg cacgcggccc gccggcagct
     8461 tttcgctgga gtgttgccaa aggaagtggc cgtcgcgctc gcgaaacgcg agcaggcagc
     8521 ccagatcgac gtccgccggc gcacgcttca agtacccagt ggcgttgttg gtgccgacga
     8581 agaccttgcc accagcaatg gttggactgc cataggtctg gctgcccagc gcggctgtcc
     8641 acttcacatt gcggctcgtc ccgggcagcc acaccccggt gcggcggtcg aagtcgccag
     8701 gattccaatc ctccggtccg gcgtcggcga gagccacgtt gttccgcccg ccccagccgc
     8761 cccactgcgg ccagtcgcct tcgcggacga gtcgcgactt gcgatcgagc tgaatcggcc
     8821 gcgggccgaa ctgtgcgccg atcgcctcgg ccgcttcgcg ccgcaaggcg gacagtcggg
     8881 cctgccgcgg ctcattcagc ttcaggtcgg cgttcaagcg ccgcacggtg tcctggtaca
     8941 ggaaccacgc cggctgctgc tgcccgctat gccacagcga aacgacgcgc cgcagggccg
     9001 cggcatcggc agcgctcgcc cgctggtccg catcgtcggc gcgactgacg ctcgccgccg
     9061 cgagcagcca cgccgccgct gccaaaacca aactgcgttc gccgtgcttg agacctcgca
     9121 tgacgcacat tttaggcagg ccgcgcgctc gcggagtact gtatattgaa gaaagtgggg
     9181 ctgggaggat tcggcatgtc gcggctgatg acggtcgttt cgttatcgct tttgtgcgga
     9241 ttttccggcc aggcggccgc ccagaatcgc acgcagctgg agcaggcccg ccacaagctg
     9301 gtcgatgaga tcctggtgcc ggcgggcgtg actaatccgc gggtcgtgca ggcgatgcgc
     9361 gacacgccgc gccacgagtt cgtcagccgc gaccagatcg ccaaggccta cctcgacatg
     9421 tccctgccga tcggcgggca gcagacgatc agctcgccgc tcatcgtctc gcagatgacg
     9481 caggccctcg atccgcagcc gggcgacaag gtgctggaga tcggcaccgg cagcggttac
     9541 caggcggcgg tcctgagccc gctggtcaag gaggtttaca cgatcgagat cgtcgagtcg
     9601 ctcggcaaat cggccgaggc tcgcctgaag cgactgggct acaagaacgt gcacgtcaag
     9661 gtcggcgacg gctatcaagg ctggccccag cacgctccgt tcgacaagat catcgtcacc
     9721 tgctcgccgg agaaagcgcc gcagccgctg gtcgatcagc tggccgacgg cgggctgatg
     9781 atcgtgccgg tgggcgagcg gtattcgcaa acgctctaca tgctgcggaa gaagggcggc
     9841 aagctggagt cgctggccct cttgccgacg ctgttcgtgc cgatgaccgg caaggccgag
     9901 gagcagcggg cggtgcagcc cgacccgacc aacccgcagg tcgtcaacgg gagcttcgag
     9961 caggacgcct cgcaattcaa aaacggggcg cagcccggct ggtactacga gcggctggtc
    10021 gagtggaaga ccgacgccaa agcgcccgaa ggcgagcact ttgtcgagtt caagaattcg
    10081 gagccgggac tcggttcgca cttgctccag ggatttgcaa tcgacgggcg caaggtgtcg
    10141 cagctgacgg tgagcggaca ggtaagaact gagaacgtcg tcggcggcgc cgacgacgcc
    10201 gcgccgcaca tcgtgataac gctctacagc gagcagcgca aagacctggg caacctgctc
    10261 cttggcccgt tcaaagggac gagcgactgg caccaggaat ccaagacgtt ccgcattccc
    10321 cccgaagccc gggaagggat cctgcggatc ggcctgttcg gcgcgaccgg ggtcgcggcc
    10381 ttcgacaagg tggagatgcg aaaaacgcca acgggaaccc cccagtgaag ctgggcaaac
    10441 ggcgcagcat attcctgtgg cggcccctct gccgattccg tcgattctag cggttgtaac
    10501 gattttgacg gcgcgcgagc ggggcatctt gccgatttta ggagacgatt ccacgtcccc
    10561 tcatgctgcg cgcaaatgct gagacgtttg ctagcgatcg ctgcaatcct ggttgccagc
    10621 ctggcaggct gcaacctggc gggaccgaat cgcgcctacg acgcagagtt ggcacactac
    10681 aagcaggttg cggcgacggt cgatcacccc gagctgacga cgccccccag cgacaacatc
    10741 aatacgcccg agccgcggac gctcgaccac aacgccgcgc cgcaggagcc gcgggagtgg
    10801 tgggacatga cggtggagga ggcccttcag acggcgctgg cccgcacgag cgtcctccgc
    10861 gatttgggcg gcacggtcat ccgctcgccg gacacgacgc ggacgatcct cggcccctcg
    10921 ctgattgcga ccgagccgcg gttcggcatg gaggcggccc tcagcgagtt cgacgcgacg
    10981 ctggcctcca gcgtgatcgc cgagaagaac gaccggatga tcaacaactt cgtggcctcg
    11041 ggcggcacgc ggttcttcaa gcaggacctg atcaacttcc agtcgcagat cagcaagctg
    11101 ggcgtggccg gcacgcggat gacgctccgc ggcacgagca cctacgactt caacaacgcc
    11161 agcttcaacc gctttcccag cgtctgggac acgctggtcg aggccgaact ccgtcagccg
    11221 ctctatcaag gcgcgggggc gcggttcaac cggctggccg ggccgaacgc gattccgggc
    11281 ttctacaacg gcgtgctgat cgcccgcgtc aacaccgaca tgagcgtggc cgaatttgag
    11341 atcggcatcc gcgacgtggt gagcaacgtc gagaacgcct actgggatct gtactttgcc
    11401 taccgcgacc tggacgcccg gctgcaggcc cgcaacgtcg ccctggaagc ctggcggcgg
    11461 gtcaaggtca tcgccgacac cgagcagcag gaaggggccc tggagcgcga ggcccagctc
    11521 cgcgagcagt acttccgcta cgaagaggac gtgcagaacg cactggcagg cacgctgcaa
    11581 tcttcgacta cgaacaacaa cggctccagc ggcggcagct tccgcggctt aggcggcgtg
    11641 ctggtcagcg agcggcggct gcggctggcg atcggcctgc cgatcaccga cggccggatg
    11701 atccgccccg ccgacgagcc gacggcggcc cgcgtgactt tcgactggcc cagcagctcg
    11761 ggcgacgcca tcgcccgccg gccggaactg ctgcggcagc gcctggcggt gaagcgccgc
    11821 gaaatggagt tcgtcgcggc ccgcaacttt ttgctgccgc ggttcgactt gctcggccgc
    11881 taccgcgtgc ggggcctggg caaagccctg atcggcgatc aagtgctccc ggtcgacccg
    11941 gctgatccgg aaggcctgtt cgagaacagc tcgctcaaaa acctggccct gggcaatttc
    12001 caggagtggc agctgggcgg cgaagtctcg ctgcccatcg gcttccgccg cggccacgcc
    12061 gccgtgcgaa acgctcagct cttcgtcgcc cgcgagaagg cggtgctgga ggagcaggag
    12121 cggcaggtga ttcacgacct gagcaacgcc ctgggcgacg tcgatcgcac ctacaagatc
    12181 atgcagacgg cctacaaccg ccgcctggcc accgtggaca acgtcaacat cgtccgcacg
    12241 cggatcgaga ccgggcaaaa cgtcacgccc gagcaactgc tcgaatccgg gcggcggctg
    12301 gcggaggccg acatccagta tcacaaggct ctggtcgagc acatgctggc gatcaagaac
    12361 gtgcacttcg aaaagggcac gctgctcgaa tacgggcaca tctacctggc cgagcaggtt
    12421 tccatcgtca acgcccgtcc cccgcccgcg gacgtgcagc agccgacgcc cgcgcagccc
    12481 gccgagggaa ttccaccgcc tccggtcgaa gtcatgcccg cgccgcagcc ggccccgccg
    12541 ccgcagccga tgccgccgcc accgccggca accggcgcag cgccggctgg aacgggccca
    12601 gcgccgattg gtacgggtgc agcgccgcgg ctccctctcc ctctgggaga gggctggggt
    12661 gagggtctgg acgcacttcc ggtccagggc ggcttgcctc cggctcctgc ccagccgcag
    12721 tcgccgccga tcgtcgttca gccgccgcaa gcgccaccgg tggaaggact gccgcctgcg
    12781 ccctcgcagt cgagtgggcc cgcgctagcg ccgccggccg cagaaacttc gtcggcgccc
    12841 agcggcctgc cgacgcttcc gtccaccggg cgagcgccaa tcttgcagtg gtcgcgccgc
    12901 cggcagccgg aacagccggc gaacgtcaac gtcccggcta acccgttcat gctcgaggtg
    12961 cggccgcagc ccgagcttgg cctgccgcct gctaatcaag caccatcgcc gccgccgctg
    13021 ccgccgccat cgcggcaggt cgcgccagcg ccctcgccgc cgccgcaagc gcctcggcct
    13081 tcggcggcct tcatcgagcg actgtggtat tcccacgagc cggccaccac gcggctgccc
    13141 aacgtcgatg tcgttgtgcc tgtgcaagcg ccagtcgcgc cgccgacgcc tgccgcctgg
    13201 cagccgccgg tgatggatca gccagtcccg acgacccgcc tgccgcagcc ggaagttgaa
    13261 ccgccgcccg ttctggtgca gcctgcggcg cccgtgcccg cccccgcgat gcgcctgccg
    13321 gagccggcgc ccgagccgcc gccggtgcgc ttgccgctgc cgccggctta tccgccgatc
    13381 gaccccgccc agcgcctgcc gcagcccgca cccgaggccc gcgcggaaaa cgagattcag
    13441 cgcctgccgc tgccctatcc gtaggcgaga attgccagcg tgcggtagag ttacaggcga
    13501 aggcaggccg cgccatgaat atcggttctc caccaccgct cgacgatttc gcgccggccg
    13561 aggcctggca ggagatcgag tcggccctgg aggaactggc ccagctctcg cgccgcgacc
    13621 tgcccccagg cgagtttcac agccggctca tggagcgcat gacaggagtc ctggccgcgg
    13681 tcggcggcgt cgtctggagc ttcggcagcg aggggcggcc cgcgatcgag tgccagctgc
    13741 atctcgatca atcgctcgcc ggcaatgcgg cggagctggg ccgccaccag cgcctggccg
    13801 agtcggtcgc ggctgccggc gaagcgcggc tcgtcccgcc cgcctttcgc gacgcccagc
    13861 acgccaatgc ttcgccctgg ctggcgatcc tctgtccgat ttcgcccgac ggcgaggcgc
    13921 ggtacgtcgt cgaagttttt cagcggcccg atggccgcag cgccgtcgag gagggatatc
    13981 tgcggctggt gaggacggcc tgcgacatcg ccgagcaatt ccaccgcggc cgactgctcc
    14041 gcgagctgaa agcgaatcaa ggcgagctgg cctcgctggt cgaatactcg gcgctgatcc
    14101 accaacggct cgacctgcgg ctaacggcgg cggcgatcgc gaatgaagcc cgccgcgtgt
    14161 tgtcgtgcga ccgcgtgtcc gtgctggcct gcgcggggcg gcatcccaaa gtggcggcga
    14221 ttagcggcgt ggagacgttt gatcggcgga gcggtatcgt gcgggcgctg gaggcggtcg
    14281 cgcggccggt cgtcgccgtc ggcgagccgc tctgctatcc cgccgacgcc gacgaattgc
    14341 cggagccggt cgccgagcaa gtgcaaacac ttgtggacca atcgcacgcc cgcgggttgc
    14401 tcgtgatccc gctgctgaac ggcccggccg atgccgcgcg gaccattggg ctgctggcaa
    14461 tcgagcaatt cgggcaggag atcgattcgg gactgcggaa gcgagcggcg gcgctggttg
    14521 aaacaagtgc ggcggcgctc agccaggcga tcgaatacga acggattccg ctgcgcgggc
    14581 cgctcacctg gctggcggcc gttctcggcc tcggcccagg ccgccgctgg tctcccgcgc
    14641 tgacggcgat tgcagtcctg gcagccgcgg tcatcgcttt ggccgtgatt cccgtggagt
    14701 tgacggtcga agcccgcggg cggctgatgc cggttcggcg gcaacacgtg tttgccccca
    14761 gcgacgccgt cgtcgtggaa ttgacgcagc aaggcggggc ggaagtccag ctcggccaga
    14821 cgctggcgaa gctgcacagc cccgcgctcg acatcgccca gagcgagctg gtcggcaaac
    14881 agcgcacggt gcaggaggaa ttgctggccg cggagacgga attcctgcgc ggcgagaccg
    14941 agggcaagtc gcccgcgtcg cgcacgcagc tggccggccg agtgcagcag ctcaaagaag
    15001 agctgcgcgg cctggacgcg cagctgcaaa tcgtgcggca gcaactgaag gagctggaga
    15061 tcaccagccc gctggccggc gtcgtgatta cctgggatgc tcagcggcag ctcgccggcc
    15121 gaccagtcaa gcgcggcgat tcgctgctga ccgtggccga cctttccgga ccgtgggagc
    15181 tgctgctcga cgtgcccgac gaccgtgccg ggccgattct cgagaccagc gggaagccgg
    15241 aagaaatgca agtgactttt cagctgggga ccgatccggg cgtggtgcgc gaggccaagg
    15301 tcaaggaagt ctcgcccgcg acggagttgt cgccggaaaa cgcgccgagc gtgctggtaa
    15361 cggctgccct gccccaaacg gagaagctga cgctgcggcc cggcgcgtcg gtggtggctc
    15421 gcgtgcactg cggccgccgt tcgctgggct acgtttggtt gcatgagttc tgggaggcgg
    15481 tgcgactgag gctctttctt tgaatggacg tcgaaccatt gacgcagagg gcaaagagga
    15541 caacgaaaga acatgcatac tcacctcatt tctttgcgcc tctgcggctt tgcgccttgg
    15601 cgtcaatcgc tgtacctctg cgcggcggtt gtgtgttttt cgcaagcgtg cggcgcgcag
    15661 gagatcaaga tcgccggagt attggtgaag ctgatcgacc agctggatgt gccggcccgc
    15721 gagcagggaa caatcgcgca actggcggtg caggagggat cgcgggtcaa ggccgacgac
    15781 ctgctgatgc ggatcgacga caccgaggcc cgctatgcgg aagatcgcgc ccgcgtcgag
    15841 atggccgtag cggcccagca ggcggccagc gatgtcgccg agcggctggc cgagcggacg
    15901 ctggccactg ccgagggcga gctgcggcgg gccgacgaag cccgcgcgaa gctgcgtaac
    15961 atcgtgaccg aggcggagta cgagaagctc cgcctggccg tcgatcaagc caagctcgcg
    16021 gtcgaaaagg cgcagcagga ccgctccgta gcggcgctgc agcgcgattt gaaaaaggtc
    16081 gagctggaat tcgccgcccg ccagaccgcc cgccaccagg cgaccgcgcc cttcgccggc
    16141 gtcatcgtgc aggtccacaa gcggcaggga gactgggtcg agcccggcga caaaatgctg
    16201 cggctgattc gcctcgaccg tttgcgggtg gaaggattcc tcgacgccgc ccaggccaag
    16261 tccagcctcg aaggccggcc cgtcgcgctg acgctcgacg ctccggctag ccccggcacg
    16321 gcgttcaccg gcaaattgat cttcgtcagc cccgagatcg atcccatcaa tcgccaggtc
    16381 cgcgtgctgg cggaaatcga gaacccgacc ttcgcgctcc aacccggctc gcgcgggacg
    16441 ctgacgatcc ggtagccaac atgctcgcca gcgcgactgt cgatcgtcca ctgctgcttc
    16501 ggcagcgccg cgacctggtg gtacgcgagc agtggttcgc cggccagcga cactttgtcc
    16561 tccacgatcc gctggcgctg acgtacgcgt acctgaccga gatcgagcac gcggtgtggg
    16621 cgctgctcga cgggaggacg
//
LOCUS       DASRJB010000818         9446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_799090, whole genome shotgun sequence.
ACCESSION   DASRJB010000818 DASRJB010000000
VERSION     DASRJB010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799090"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3409
                     /locus_tag="VFB80_21480"
     CDS             <1..3409
                     /locus_tag="VFB80_21480"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN36422.1"
                     /translation="DADGLPVEIAGTGAFGVNDANDDERIRIPAVAGQTYFLRIKGAA
                     LGNLNSPAINIYNVSVINTPAPVPFDLELDDRIIDGAVAAGAPLTASQFQSNLAGLFP
                     DDFFNGKVLSFKFDTTTLGIRGEEAVIADYIGATGRFIFAFPFSAAPVVGDTFQVESV
                     DTGRNNNDNITRDNTPTIFLRVPDVITLAGVATLDDVPFNGTAPGDPPDQFIGIPHIS
                     SVLLDVPLASGAGFRVPIFVTENGTSNQTTALVLAGYAQPVDVTNRPGLFSFTFGSAG
                     SFITSLTPDGSYFISARTEMIDPANPQQAQGYGAFAQSLEIFVDTTEPPVSFGDPGID
                     GDGLTADSDSFVIPNPDTIFDLVTNDLTPTFWGRAEADATIRLFADVFFDLNGNGEFD
                     MGIDVAPNGVFDPNIDVFIGQDTAIPLDGNQQEPNGFWKIESVINFNDPRFFIGLGGT
                     RTVFVTAEDVAGNVNDNVTPQELDIFIDVQGPQIIDVTINAPSGSIDDVYDLFDPKPS
                     TDGPTPLVNSIFIEIVDFPARDLPAFDSPAFKEDVAENPGHYLVTGDYNGIIPILDVE
                     ATLDAVVDGQPATGRVELIFRKPGADGIFNTADDIGAPLPDDRFTLFVNDDGIIDLAG
                     NKLDGESNADEPHDSPPPSPDFPDVLGVDGVPTGDGIPGGDFIARFTVDSRPEVAVWA
                     AGSVWVDINGNNQFDPNNTDFTNRDITYMLGLTSDDLFAGKFEPTGAEDAGVALFDKL
                     GAYGRFHGAFRWLIDTNNNGVPDIEVVDPAQVNGLPVAGNFGANLGDEVGLFTGTQWR
                     LDTNHNFQVDTTINWPRPGYPIVGDFDGDGVDDLATWTDDVFAFDLSSVGAPGPVAAL
                     NTLTNGVPTGVNGSIEREFKFGFIGPGEQPVTADMNQDGIEDVGLWVPARDGVPPTEG
                     AEWYFLISGVVNNNTPPGVPPQIGPSITGGAYAPGDGPGEYLGFATYGLVAAAYSNGR
                     IVDDPLIAGPGNIVRFEPTPFGKDNYIQYGDQFALPIVGNFDPPVTATGTVGELQEER
                     DPLDVNADGSISPIDALLVINYINAGGVAAPTPGPAGPYLDVNDDNFVSPIDALIIIN
                     FLNNQPNGGGGEGEEGEGEAADAFFGDPGLMLDLLGNDPPPSGRRRR"
     gene            complement(3487..4989)
                     /locus_tag="VFB80_21485"
     CDS             complement(3487..4989)
                     /locus_tag="VFB80_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN36423.1"
                     /translation="MNTLLRLSAVAILAAGTAAAQEQKPLVRVGSKASIENVTLGELL
                     THLVNSSGAEARHRAALGGTQIAFQALSKGDIDVYPEYTGTLTAEILQGEQVRSDEDL
                     RAALARRGLSLSRPLGFNNTYALGLPDVAAKRLNLRKISDLARPEHAGLRLGFSEEFM
                     NRADGWPGLQAAYQLPHRPQSLDHSLAYRGLAAGSIDLTDLYSTDAEIAYYKLRLLED
                     DRGYFPEYHCVLLFRNDLANRAPQVLAALARLEGAIDNAAMSEMNTRVKTDHIRERQV
                     AADFLASKLGMKVELRREPNRLWAATQGHLLLVGVSLAAAVLIALPLGIWSYKWPRLG
                     HGIMALVGIVQTLPSMAVLACMLTFLPIGPAPAIVALFFYSLLPIVRSTLTGLKEIPN
                     HLKESAIVLGLSPRARLWLVELPMASRSILSGIKTAAVINVGTATIGSLVGAGGYGDA
                     ILAGIRLVDIPLILQGAVPAAVLALLVQGGFDLLEPLVVPKGLRLAAGKS"
     gene            complement(4986..5738)
                     /locus_tag="VFB80_21490"
     CDS             complement(4986..5738)
                     /locus_tag="VFB80_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN36424.1"
                     /translation="MLELIDVAIKFGRLQVLQPTSVRFERGKTTVLIGPSGCGKSTLL
                     RIMIGLVEPDQGQVLIDGQPLTPASVLSLRRKIGYVIQDGGLFPHLSALDNVGLLARH
                     LGWTRLEVRSRVEKLAELTRLPQEALAKYPAQLSGGQRQRLGIMRALMLDPDVILLDE
                     PMGALDPLVRYDLQEDLRKIFQTLGKTVVMVTHDMGEAGFFGDRVLLLGSGCIVQDGT
                     LDELIAAPADDFVRRFISAQRLPGLNGKEVAP"
     gene            complement(5740..6960)
                     /locus_tag="VFB80_21495"
     CDS             complement(5740..6960)
                     /locus_tag="VFB80_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN36425.1"
                     /translation="MNRQPFQTPPRVWQPRMTPWLVRLWRPLINRELHREQKIVQIDV
                     AGIEHVQRALAGGTGVLVTPNHSFHYDSYVLIEAAHRVGTPFHFLTAWQVFAMSKWHE
                     QLMLQWHGCFSINREAADLGAFKTAVEILRTSREPLVIFPEGDIYHNNDRVTPFRDGA
                     AAIAMSAAKKAERQIVCVPAALKCFYVDDPSESLQRLMARLEESIHWRPRPELPLAER
                     IYRFAEGLLALKEYEYLGGARSGAVKERTQALADAVLSRLEQRHSVPNKGGIVPERVK
                     EVRRAVIKAVEQDGQSPDAKQQFAADMDDLFFVIQLFSYPGDYLAEQQSIERIAETLD
                     KFEEDVLKADYPGIRGTRRAIVRFGEPIPVPKEREAKGSVEQWTDLVESHVQGLLDEI
                     NGKSNGREMTARHG"
     gene            7111..7998
                     /locus_tag="VFB80_21500"
     CDS             7111..7998
                     /locus_tag="VFB80_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36426.1"
                     /translation="MNEPAAALDDALNQWLCGLTLQAPARPAQAAERLRRRWSWRLSL
                     LLHGSVFAFALAAFLADVPLAAYSGSAEATSRPAGEFTDAAPVLVTIEGLQAARPTAA
                     ELAESEIARLAAELPPPELAAELLDPAAASTSAASGWLHRRVLDEVAKAERLSQDDQR
                     ERLAALGGELEQISSAESVDAISRRLSGFFGTAGRATQPATEPVPGEFDYDTAQLHDV
                     KRTERADGGYDYVAVLLDAAGRTMESALAEAEGEQLYKTFQLIKANPLLERVYRGVVM
                     SLFDKLMRQQPPVREERQE"
     gene            complement(8004..8423)
                     /locus_tag="VFB80_21505"
     CDS             complement(8004..8423)
                     /locus_tag="VFB80_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36427.1"
                     /translation="MRRLLALATSVILISAGCGMDSGATLTGSVTYEGTPLAKGSITL
                     TPSDGKGPIAGGEITAGRYSISGLVPGKKQVQIAAAEESGPPLSSADLANAAKSGKPA
                     APPPKEIVPPNAVGNGAIIEVQAGTHTHDFALKKPGS"
     gene            complement(8453..>9446)
                     /locus_tag="VFB80_21510"
     CDS             complement(8453..>9446)
                     /locus_tag="VFB80_21510"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36428.1"
                     /translation="VVIAIIGVLVALLLPAVQSAREASRRTKCMNNLKQQALAAHNHE
                     DTYKAFPYARKYDRWDTYTWSQKILPFIEQQNVYENYWTMPTVPWVQNYPGPNGPIGN
                     DARLRSARHTKLPCWFCPSDRGTFNNEITTNEYGFVRGNYRGCTGTGDMYGGVPNQFT
                     AATAWYKGVFSVTAGQSVDPTQSWATIQPVQVRAGEVTDGLSNTLMFSEGIVPDVPGW
                     GGPIGSHIYGNMGGALFTATLTPNSTAQDRLVGPCPQNQGDARYKAPCGSFQGNAWWT
                     PSAAGAHAAARSFHPNGVNASLADGSTQFYSNNIDLAVWRALGSRDGGESVSAP"
BASE COUNT         1654 a         3137 c         2977 g         1678 t
ORIGIN      
        1 tgacgccgac ggcttgccgg ttgaaatcgc cggcaccggc gcattcggag tcaacgatgc
       61 caacgacgac gagcgaatcc gcattcccgc cgtcgcgggc cagacgtact tcctgcggat
      121 caagggcgcg gccctgggga acctcaattc gcccgcgatc aacatctaca atgtctcggt
      181 catcaatacg cccgccccag tgccgtttga cctggagctg gacgaccgca tcattgacgg
      241 cgccgtggca gccggcgctc cgctgaccgc cagtcaattc cagagcaacc tggccggact
      301 gttcccggac gactttttca acggcaaggt gctgagcttc aagttcgata cgaccacgtt
      361 gggcatccgc ggggaggaag ccgtgatcgc cgattacatc ggcgctacgg ggcgattcat
      421 tttcgccttc ccgttttcgg cggctccggt ggtgggagac accttccaag tcgagagcgt
      481 cgataccggc cgcaacaaca acgacaacat cacccgtgac aatacgccca cgatcttcct
      541 ccgcgtgccg gatgtgatca cgctcgccgg cgtagccacg ctcgacgacg tgcccttcaa
      601 cggcacggcg ccgggcgatc cgcccgacca gttcatcggc attccgcaca tcagcagcgt
      661 gttgcttgac gtgccccttg cgtccggcgc cggtttccgc gtgccgattt tcgttacgga
      721 gaacggtacg agcaatcaga ctactgcgct cgtgctggcc ggctacgccc agccggtcga
      781 tgtgacgaat cggccgggcc tgttcagctt tacgtttggc tcggccgggt cgtttatcac
      841 ttcgctgacg cccgacggga gctatttcat ctcggcgcgc accgagatga tcgatccggc
      901 caacccgcag caggcgcagg gttatggcgc ctttgcccaa tcgctggaga tcttcgtcga
      961 tacaaccgag ccgccggttt cgttcggcga tccgggcatc gacggagacg gcctcaccgc
     1021 cgacagcgac agcttcgtca ttcccaatcc cgatacgatc ttcgacctgg tgacgaacga
     1081 cctcacgccc accttctggg ggcgagccga ggcggacgcc accatccgcc tgtttgccga
     1141 cgtgttcttt gacttgaatg gcaacggcga atttgatatg gggattgacg tggcgcccaa
     1201 cggcgtcttc gatccgaaca tcgacgtgtt catcggccag gacacggcca ttccgctcga
     1261 cggcaaccag caggagccca acggcttctg gaagatcgag tcggtcatca atttcaacga
     1321 cccgcggttc ttcatcggcc tcggcggtac gcggaccgtg ttcgtcaccg cggaggatgt
     1381 cgccggcaac gtcaacgaca atgtgacgcc gcaggaattg gacatcttta tcgatgtgca
     1441 gggcccgcag atcatcgacg tcacgatcaa cgccccgtcg ggcagcatcg acgacgtgta
     1501 cgatctgttt gatcccaagc cttcgaccga tggcccgacg ccgctcgtca actcgatctt
     1561 catcgagatc gtggacttcc cggcccggga cctccctgcc ttcgattcgc cggcgttcaa
     1621 ggaagacgtc gccgagaatc ccgggcatta cctggtgacg ggcgactaca acggcatcat
     1681 tccgatcctg gacgtcgagg ccactctgga cgccgtcgtg gacggccagc ccgccaccgg
     1741 gcgcgtggag ctcatcttcc gcaaacccgg cgccgacggc atcttcaaca cggccgacga
     1801 catcggtgcc ccgctgccag acgaccggtt cactctgttc gtcaatgacg atgggatcat
     1861 cgatcttgcc ggcaataagc tcgatggcga gagcaatgcc gacgagccgc acgacagtcc
     1921 gccaccgtcg cccgacttcc ccgacgtgct gggcgttgat ggcgtaccga caggcgacgg
     1981 cattcccggc ggcgacttta tcgctcgctt cacggtcgat agccggccgg aagtcgcggt
     2041 ctgggcggcc ggcagcgtct gggtcgatat caacggcaac aaccagttcg atccgaacaa
     2101 tacggacttc accaaccgcg acattaccta catgctgggt ctgaccagcg acgacctgtt
     2161 cgcgggcaag ttcgagccca ccggggcgga ggacgccggc gtcgcgctgt tcgacaagct
     2221 cggggcctat ggccgcttcc acggcgcgtt ccgctggttg atcgacacca acaacaacgg
     2281 cgtgccggac atcgaggtcg tcgatccggc ccaggtcaac ggcttgccgg tcgcgggcaa
     2341 tttcggcgcg aatctcggcg acgaggtcgg cctgttcacc ggcacgcagt ggcggctcga
     2401 cacgaaccac aacttccagg tcgataccac gatcaactgg ccgcggccgg gatatcccat
     2461 cgtcggcgac ttcgacggcg acggtgtgga cgacctggcc acctggaccg acgacgtctt
     2521 tgccttcgac cttagctccg tcggggcgcc gggaccggtc gccgccctga acactcttac
     2581 caacggggtg cccacgggag tcaacggatc gatcgagcgc gaattcaagt tcggcttcat
     2641 cgggccgggc gaacagccgg tcaccgccga catgaaccag gacggcatcg aagacgtggg
     2701 cctgtgggtt ccagctcgcg acggcgtgcc gccaaccgag ggcgccgaat ggtacttcct
     2761 gatctcggga gtggtgaaca acaacacgcc gccgggggtg ccgccgcaga ttggtccttc
     2821 gatcactggc ggcgcgtatg cccccggcga cggaccgggc gagtacctgg gctttgctac
     2881 gtatgggctg gtcgccgcgg cctacagcaa cggccggatc gtggatgacc cgctgattgc
     2941 cggccctggc aacatcgtcc gctttgagcc gacgccgttt ggcaaggaca actacattca
     3001 gtacggcgac cagttcgcgc tgccgatcgt cggcaacttc gatccgcctg tgaccgccac
     3061 gggcaccgtt ggcgagctgc aggaggagcg cgacccgctg gacgtgaatg ccgacggcag
     3121 catctcgccg atcgacgcct tgctggtcat caactacatc aacgccggcg gcgtggcggc
     3181 tccaactccg ggaccggcgg ggccgtacct cgacgtcaac gacgacaact tcgtctcgcc
     3241 catcgacgct ttgataatta tcaacttcct gaacaatcag cccaacggcg gcggcggcga
     3301 aggtgaagaa ggcgaaggcg aagcagccga cgcgttcttc ggagatcccg gcttaatgct
     3361 ggacctgctc ggcaacgatc cgccgccgag cggacgaagg cggaggtagt ctcgcagcgc
     3421 agactgccgg aatgaagggc gatgaatcgc ccggggccgg tcgccaaagc ggccggcccg
     3481 cggcgtctag ctcttgccgg ccgccagccg caagcccttc ggcacgacga gcggctcgag
     3541 cagatcaaac ccgccctgca cgagcagtgc caagacggct gccggcacgg cgccctggag
     3601 aatcagcggg atatcgacca ggcggatgcc ggccaggatc gcgtcgccat agccgcctgc
     3661 accgacgagc gagccgatgg tcgccgttcc gacgttgatc accgccgccg tcttgatccc
     3721 cgacaggatc gagcgcgagg ccatcggcag ctcgaccagc cacaggcggg cccgcggcga
     3781 gagcccaagc acaatcgccg actccttgag gtgattgggg atttccttga gccccgtgag
     3841 cgtgctgcgg acaatgggca gcaggctgta gaagaacagg gcaacgatcg ccggcgcggg
     3901 cccgatcggc aggaacgtga gcatgcacgc cagcacggcc atcgacggca gcgtttgcac
     3961 gatgccgacc agcgccatga tgccgtgccc cagccgcggc catttgtacg accagatccc
     4021 cagcggcagg gcaatcagga cagccgcggc cagcgacacg ccgaccagca gcaaatgccc
     4081 ctgcgtcgcc gcccacagcc gattcggttc ccgtcgcagc tcgaccttca ttccgagttt
     4141 gctcgccagg aagtcggcgg cgacctgccg ctcgcggatg tggtcagtct tcacgcgcgt
     4201 gttcatctcg ctcattgccg cgttgtcgat cgccccttcc aaccgggcca gcgccgccag
     4261 cacttgcggc gcgcgattgg ccaggtcgtt gcgaaagagc agcacgcagt gatactcggg
     4321 aaagtaaccg cggtcgtcct ccagcagccg tagcttgtag taggcgatct cggcgtcggt
     4381 cgagtagagg tcggtcaggt cgatggaccc cgccgccagc ccgcgatacg ccaggctgtg
     4441 gtcgagcgac tggggccggt gcggcagttg atacgcggct tgcagccccg gccagccgtc
     4501 ggctcggttc atgaattcct cactaaagcc gagccgcagg cccgcgtgtt cgggccgggc
     4561 cagatcggaa atctttcgca ggttcagccg ctttgccgcg acgtccggca aaccgagggc
     4621 gtaggtgttg ttgaatccca gcggccggct gagggacagc ccgcgacgag ccagcgccgc
     4681 ccgcaggtcc tcgtcgctgc ggacttgctc gccctggaga atctcggccg tcagcgtgcc
     4741 ggtgtattcg ggatagacgt cgatgtcgcc cttgctgagt gcctgaaagg cgatttgcgt
     4801 gccgcccagc gccgcgcggt gccgcgcctc cgcgccgctg ctgttgacca gatgcgtgag
     4861 caattcaccc agggttacat tttcgatcga ggccttcgag ccgactcgca ccagcggctt
     4921 ttgttcttgg gccgccgccg tgcctgcggc cagaatagcc accgccgaaa ggcggagcag
     4981 cgtgttcatg gcgcgacctc ctttccgttt aaccccggca gccgctgggc gctgatgaac
     5041 cgtcgcacga agtcgtcggc cggcgcggcg attaactcgt cgagcgtgcc gtcttgcacg
     5101 atgcagccgc tgcccaagag cagcacgcgg tcgccaaaga agccggcctc gcccatgtca
     5161 tgcgtaacca tcacgacggt cttgccgaga gtctggaaga tcttccgcag gtcctcctgg
     5221 aggtcgtagc gcaccagcgg atcgagcgcg cccattggct cgtcgagcag gatcacgtcc
     5281 gggtcgagca tcagggcccg catgattccc agccgctgcc gctgcccgcc ggagagttgc
     5341 gccggatact tggccagcgc ttcctgcgga aggcgcgtca gctcagccag cttttccacg
     5401 cggctgcgga cctccagcct ggtccagccc aggtggcggg ccaagaggcc gacgttgtcc
     5461 agcgccgaga ggtgcggaaa cagaccgccg tcctgaatga catagccgat cttccgccgc
     5521 agcgagagca cgctcgccgg tgtcagcggc tgcccgtcga tcagcacctg cccctgatcc
     5581 ggctcgacca gtccgatcat gattcgcagc agcgtggact tgccgcagcc gctgggcccg
     5641 atcagcaccg tcgtctttcc gcgctcgaag cgcaccgacg tcggctggag cacctgcagg
     5701 cggccaaact taatcgctac gtcgatcaac tcgagcatct caaccgtgcc tcgccgtcat
     5761 ttctcggcca ttggatttgc cgttgatttc atctaagagc ccctgcacgt gactctccac
     5821 gaggtcggtc cattgctcga cgctaccctt cgcttcgcgc tccttgggga cggggattgg
     5881 ttcgccgaac cgcacgatcg cccgccgcgt gccgcggatg ccgggataat cggccttgag
     5941 cacgtcctcc tcgaacttgt cgagcgtctc agcaatccgc tcgatcgatt gctgctcggc
     6001 caggtagtcg cccggatagc tgaagagttg gatcacgaaa aacaggtcgt ccatgtcggc
     6061 ggcgaactgc tgcttcgcgt ccggactctg cccgtcctgc tccaccgcct tgatgacggc
     6121 ccggcgaacc tccttgacgc gctcgggcac gatgcctccc ttgttgggca cggaatgccg
     6181 ctgctcgagg cgcgacagca ctgcgtcggc cagcgcctgg gtccgctcct tcaccgcgcc
     6241 gctgcgggcc ccgcccaggt attcatattc cttgagcgcc agcagccctt ccgcaaagcg
     6301 gtagatccgc tcggccagcg gcaattccgg ccgcggccgc cagtgaatcg actcctccag
     6361 ccgcgccatc agccgctgca ggctctcgct gggatcgtcc acgtaaaagc acttcagcgc
     6421 cgcaggtacg cagacgattt gccgctccgc cttcttggcg gccgacatgg cgatcgccgc
     6481 cgcgccgtcg cggaacggcg tcacgcggtc gttgttgtgg taaatgtccc cctccggaaa
     6541 gattactagc ggttcgcggc tagtccgcaa aatttcgacc gccgtcttga acgcgcccaa
     6601 gtccgccgcc tcgcggttga tgctgaagca gccgtgccac tggagcatca gctgctcgtg
     6661 ccacttgctc atggcaaaca cctgccaggc cgtgaggaag tggaacggcg tgccgacccg
     6721 gtgggctgcc tcgatgagca cataagagtc gtagtggaac gagtggttgg gagttaccag
     6781 cacacccgtg cctccggcca gcgcccgctg gacgtgctcg attccggcca catcgatctg
     6841 gacaatcttc tgttcgcggt gcaactcgcg attgatcaat ggacgccaca gccgcaccag
     6901 ccacggcgtc atcctgggct gccagacgcg aggcggagtc tgaaagggct ggcggttcac
     6961 ggctgcgaaa gaatggagaa tcagcggcca gagtggccaa caccgcgcga tccagccgat
     7021 tgttttaggg gcctgcgcgg caaatagcaa catcgacgcc agctagagcc cggcagtaca
     7081 atagatccgt tgctttcgtg catttgcgcc atgaatgagc ccgcggctgc tctcgacgac
     7141 gccctgaacc agtggctctg cggcctgacg ctgcaggcgc cggcgcgtcc cgcgcaagcg
     7201 gctgagcgcc tgaggcggcg gtggagctgg cgcctgtcgc tgctgctaca tggctcggtc
     7261 ttcgcatttg ctctggcggc attcctggcc gacgtgccgc tggcggcgta ctcgggctcg
     7321 gccgaagcga ccagccggcc ggccggtgag ttcacggacg cggcaccggt gctggtcacg
     7381 atcgaaggcc tccaggctgc gcggccgacg gcggcggaac tcgccgagtc cgagatcgcc
     7441 cgcttggccg ccgagttgcc tccgcccgaa ctggctgcgg agctgctcga tcctgccgcc
     7501 gcttcgacaa gcgctgccag cggctggctg caccggcgcg tgctcgacga agtcgccaag
     7561 gccgagcggc tgagtcagga cgaccagcga gagcggctgg ccgcgctcgg cggcgagttg
     7621 gagcagatct cctcggccga atcggtcgat gccatcagcc gcaggctcag cgggttcttc
     7681 ggcaccgcag gccgggccac gcagcccgcg actgagcccg tgccgggcga attcgactac
     7741 gacacggcgc agctgcacga cgtgaagcgg accgagcgcg ccgacggcgg ctacgattac
     7801 gtcgcggtgc tgctcgacgc cgccggccgc acgatggagt ccgcacttgc cgaggccgaa
     7861 ggcgagcagc tctacaaaac cttccagctc atcaaggcca atccgcttct cgagcgggtc
     7921 tatcgcggag tggtgatgtc gctgttcgac aagctgatgc gacagcagcc acccgtccgc
     7981 gaagaacggc aggaatagga atctcagctg cccggctttt tcaaggcgaa gtcgtgcgtg
     8041 tgggttccag cctgtacttc gatgatcgcg ccgttgccga cggcgttggg cgggacaatt
     8101 tctttgggag gcggcgcggc gggcttaccg gatttggcgg cattggccag gtcggcgctg
     8161 ctcagcggcg ggccagactc ctcggcggct gcaatctgaa cctgcttctt gccgggcacg
     8221 aggccggaga tgctgtaccg acccgcggtg atttcgccgc cggcgatggg acccttgccg
     8281 tcgctggggg tcagcgtgat gctccctttg gcgaggggcg tgccctcata ggtcacgctg
     8341 ccggtgagcg tcgcaccgga atccattccg cagccagcgc tgatcagaat cacgctggtc
     8401 gccagcgcca acaagcgacg catgccggat ccctcttttt tagcctgttg aactagggcg
     8461 cgctcactga ctcgccaccg tcgcgcgaac ccagcgcccg ccagacggcc aggtcgatgt
     8521 tattcgaata gaactgtgtc gagccgtcgg ccagggacgc attcacgcca ttgggatgaa
     8581 agctgcgggc cgcggcatgc gccccggcag cgctgggagt ccaccaggcg ttgccttgaa
     8641 acgaaccgca aggcgccttg tatcgtgcgt cgccctgatt ttgggggcag ggaccgacca
     8701 gtcggtcttg cgccgtggag ttgggcgtca gcgtagctgt gaacaaggcg ccgcccatat
     8761 tgccatagat gtgagaccca attggaccgc cccatcccgg cacgtcggga acgattccct
     8821 ccgaaaacat caacgtattc gagaggccgt ccgtgacttc gccggcccga acttgaacgg
     8881 gctggatcgt cgcccaggac tgagtcggat ctaccgactg gccggctgtt accgaaaaga
     8941 ctcccttgta ccaggccgtc gccgccgtga actgatttgg aaccccgcca tacatgtcgc
     9001 cggtcccggt gcagccgcga tagttgccgc ggacgaaccc gtattcgttt gtcgtaatct
     9061 cgttattgaa agttccgcga tcgctgggac agaaccagca aggcagtttg gtgtgccttg
     9121 ccgatcgaag tcgggcgtcg ttaccgatcg gtccgttggg accgggatag ttctgcaccc
     9181 agggaaccgt cggcatagtc caatagttct cgtatacatt ctgttgctca atgaatggca
     9241 gtatcttctg gctccaggtg tacgtgtccc agcgatcgta tttgcgggcg tagggaaagg
     9301 ccttataggt gtcctcgtgg ttgtgggcgg cgagcgcctg ttgcttgagg ttgttcatgc
     9361 acttcgttcg ccgcgatgcc tcgcgggccg attgcacggc cggcaacagc agcgcgacca
     9421 gcacgccgat gatggcgatg accacc
//
LOCUS       DASRJB010000819         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1092925, whole genome shotgun sequence.
ACCESSION   DASRJB010000819 DASRJB010000000
VERSION     DASRJB010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092925"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2835)
                     /locus_tag="VFB80_21515"
     CDS             complement(<1..2835)
                     /locus_tag="VFB80_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36429.1"
                     /translation="MNDSQQLREQMWDLVYGLLTPEESQSLIARVKSDPQAARLYAEV
                     RLQADLVGQAARIEDPELVFQVEPASQPEPPMPARRPAAAASRQRPPRTRQWLAGIAG
                     TALAALIAAGIFWPQPDPQKVAANHWVTEVSAPDSMQAGVSRKLTVKTYPVSLEDHVS
                     EGGSAEVQVAVVDVTNEVRFKQVVRTDDSGRAEFEVPGQALQPGARLEVAAAQKLAEG
                     EIHGTRARGENRAAQHLLALSAPLNVEREPYVTYFLPSSPVVAPGQEFQLSTWNFYPL
                     GIQPATPDVSRVTVHNANGAMVTEPMWGAKGSVVNVEVKPPPGTAPSDLTLSFRNSAG
                     AESQTRLGSLVADDYQLAYRQSGMGKAQARGRADDFRKDLASDGRSADENKKSGEADS
                     VQQVQQLKRGYAESYYFQGRYEGRAGPAKESLMDEVVAADQPVIVTVPASAADKKSKL
                     LAVVKNRGRFVASQELEADPLQRSGSGEEAAAKDGDRKYSVVLPPEADGEIEVALVNR
                     SSDPPQVIGWRRLYREPSRKLNITVVDGKDRFAPGENVKLTLQFTDENGQPAADANVA
                     VRLWNESSIRDAGEQPVMLDDVVHGRPPQPASDQVTVAGTVAAPFAPSPEPIAPPANA
                     VAGADKQLPDVLTGGKRPEGKLAATAPLESAPSLAKNIDSRNVDSKDAAAKEDALAGL
                     ERTSPGRAAQDQQEAGYFDYSTVADTDAFLASAPSGAVSVISNRDQVEAQFRQALEAA
                     QQRRRAIVATIGGVALAGTAVLLVALAIGFVSRQPARRKLFIPVLAIAAVCLLFGLVW
                     MGRGPFARLDQVAMTPSPATGNWADDAPQPKSASVSELSEMAAPQPMESAGASASPAD
                     GLPPAVSYSVPVVPATTPAPPGAPPAPTAPPAPTAAPPASVTAGPPALAGQSRPSPLA
                     VATKPAGGAVEGADPAAAPVPPAAAAPGNLP"
     gene            complement(3065..3628)
                     /locus_tag="VFB80_21520"
     CDS             complement(3065..3628)
                     /locus_tag="VFB80_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN36430.1"
                     /translation="MSVTQHARKAAANEPAAKARARGAALLEAAFNQHQAELLGTLYY
                     LVGNLEDARDALQEAFIKCWRHQEEVGQVQNLKAWIFRVALNAGRDIRETAWRRKRQG
                     LPEDESSLASPGGGPEEIVEHDERLTRLREALKQLRPEEQEVFLLRQNGELTYEEIAE
                     SLEVPTGTVKTRMRLALSRLREVLAEV"
     gene            3892..4533
                     /locus_tag="VFB80_21525"
     CDS             3892..4533
                     /locus_tag="VFB80_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36431.1"
                     /translation="MWPEAEKTQQLLADAKGGDAEAVNRLMERHRESLRRMVQLRLDQ
                     KIQRRIDVSDVVQDVLVEANRRLQDYLANPVIPYHLWLRQIAQDRIIDAHRRHRASAK
                     RSVDKERALAVPAADDHSTIQLAAQLCDRQLTPAAAATQAELAKAVEAAIAKLPDQDC
                     EIIVMRHYEQLSNQEIAQALGLTEPAASMRYLRAIRRLKELMVNPDSEASESS"
     gene            complement(4525..>4796)
                     /locus_tag="VFB80_21530"
     CDS             complement(4525..>4796)
                     /locus_tag="VFB80_21530"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZN36432.1"
                     /translation="NLWDEGSAQSRAANYAVGTTHPVYYNPANPARAVLEPGNTSEGA
                     ASVHTILGIALPIGLIPIVLLLLYMYWIMVRRPATDIVPPVPPLR"
BASE COUNT          784 a         1590 c         1560 g          862 t
ORIGIN      
        1 gggcagatta ccgggcgctg cggcggccgg cggaaccggt gcggctgccg gatcagcgcc
       61 ttcaaccgcg ccgccggcag gctttgttgc cacggccagc ggggagggac gcgactggcc
      121 ggcgagcgcc gggggaccgg cggtgacaga tgcaggtgga gcagccgtcg gcgctggcgg
      181 ggccgtcggc gcaggtgggg ctccaggcgg cgcaggcgtc gtcgcgggaa caaccgggac
      241 gctgtacgac acggcgggtg gcaagccatc ggccggagaa gccgatgcgc cggcggactc
      301 cataggttgc ggcgccgcca tttccgacag ttcgctgacc gacgccgact ttggctgcgg
      361 tgcatcgtcc gcccagttgc cggtcgctgg gctcggcgtc atggcgactt ggtccaggcg
      421 ggcaaacgga ccacgtccca tccacaccag gccgaacagc aggcagacgg cggcaatcgc
      481 cagcaccgga atgaacagct tgcggcgagc cggctgccgc gaaacgaagc cgatggcaag
      541 ggcgaccaac aggacagccg ttccggccag cgcgacgccg ccgatcgtgg caacgatcgc
      601 ccgccgccgc tgctgggcag cctccagggc ctgccggaat tgggcttcga cctgatcgcg
      661 gttgctgatc acggaaaccg ccccactcgg cgctgaggcc aagaaagcat ctgtatccgc
      721 cacagtgctg tagtcgaagt aacccgcctc ctgctgatcc tgggctgcgc ggccgggcga
      781 ggtgcgctcc agcccggcca gggcgtcctc cttggcggcg gcgtccttgg aatcgacgtt
      841 tctggaatcg atgttcttgg ctaatgacgg tgcagattcg agcggcgcgg tagcggcgag
      901 tttgccctct gggcgcttgc cgccggtcag aacgtccggg agctgcttat cggcgccggc
      961 aactgcattg gccggcggcg cgatcggctc ggggctgggg gcgaacggag ccgcgacggt
     1021 tcccgcgacc gtgacctggt cgctggccgg ctgcggcggg cggccatgca ccacatcgtc
     1081 cagcattacg ggctgctcgc ccgcatcgcg aatggagctc tcgttccaca gccgcacggc
     1141 gacgtttgcg tcggcggccg gctggccatt ttcgtcggtg aactgcagcg tgagtttgac
     1201 attctcgccc ggcgcgaagc gatcctttcc atccacgacg gtgatattca gctttcgcga
     1261 cggctcgcgg tagagccgcc gccacccgat gacctgcggg ggatcgctgg agcggttcac
     1321 gagcgccacc tcgatctcgc cgtccgcctc gggcggcagc actacggagt atttgcggtc
     1381 gccgtccttc gccgcggctt cctcgccgct tccgctgcgc tgcaaaggat cggcctcgag
     1441 ctcctggctg gcaacgaacc ggcctcgatt cttgaccacc gccagcagtt ttgatttctt
     1501 gtccgccgcg gatgcaggca cggtgacaat cactggctga tcggccgcga caacctcatc
     1561 catcagcgat tccttggccg gcccggcccg gccctcgtac cttccctgaa agtagtagct
     1621 ttcggcgtag ccgcgcttga gctgctggac ctgctgcacg gagtcggcct cgcccgattt
     1681 tttgttctcg tcggcgctgc ggccgtcgga ggccaagtcc ttgcggaagt cgtcggccct
     1741 gccgcgcgcc tgcgccttgc ccattcccga ctgtcgatag gcaagttggt agtcgtcggc
     1801 gactagactc cccaggcgcg tctgcgattc ggcgcccgca ctgttgcgga agctcaacgt
     1861 cagatcggaa ggggcggtgc cgggcggcgg cttgacttcg acgttcacca ccgacccttt
     1921 ggcgccccac atcggctcgg tcaccatcgc gccattcgcg ttgtgaaccg tgacgcgcga
     1981 aacatccggc gtcgccggct gaatgccgag gggatagaaa ttccacgtcg aaagctggaa
     2041 ttcctgcccg ggcgcgacga cgggcgagga aggcaggaaa taagtcacgt acggctcgcg
     2101 ctcgacgttg agcggcgcac tcaaggcgag caggtgctgc gcggcacggt tctcgccgcg
     2161 ggcccgcgta ccatgtattt ccccttcggc cagtttctgt gccgcggcga cttccagccg
     2221 cgctccgggt tgcagcgcct ggccgggaac ttcgaactcg gcgcggccgc tgtcgtcggt
     2281 ccgcaccacc tgcttgaatc tcacttcgtt ggtgacatcg accaccgcca cttgaacttc
     2341 ggccgatcca ccctcggaaa cgtggtcttc caagctgacg ggatacgtct tgacggtcag
     2401 ctttcggctg acgcccgcct gcatcgagtc cggggcactg acttcggtga cccagtggtt
     2461 ggcggcgacc ttctgcgggt ctggctgcgg ccagaagatg ccggccgcaa tcagcgctgc
     2521 cagagccgtc ccggcaatcc cggcgagcca ctggcgggtc cgcggggggc gctggcggct
     2581 ggcagctgcg gctggccgcc gggcaggcat cggcggctcg ggctggctag ctggctcgac
     2641 ttggaagacg agttccggat cttcgatcct tgcggcctgg ccgacaaggt cggcctgcag
     2701 gcggacctcg gcatagagcc gggccgcctg cggatcggat ttgacacgag cgatcaggga
     2761 ctggctttcc tcgggcgtta gcagcccgta gaccaggtcc cacatttgct cgcgaagttg
     2821 ctggctgtcg ttcatcggtc ccatcgcccc tggcacggtc ctgtgctctt catcggaaaa
     2881 ccgccacgct gtacctccga tggcaagctg cggccagctg aacttcctgc tcaacttggc
     2941 gcactaacgc gtaacggaat tcggtcgtgc tcgttgtagt tgtggtgcag gccacctccg
     3001 gttcggtact gccacgcccc cttaacccta gccactagcc ctaacctcca gccccagccc
     3061 tgcctcaaac ctcggccagc acttcccgca atcgcgaaag ggccagccgc atccgcgtct
     3121 tcaccgttcc ggtcggcact tcgagcgact cggcgatctc ctcgtacgtg agctcgccgt
     3181 tttgccggag caggaatact tcctgctcct cgggccgaag ttgtttcagc gcttcccgca
     3241 accgggtcag gcgctcgtcg tgctccacaa tttcctccgg cccgccgccc gggctggcga
     3301 ggctgctttc gtcttcgggc agcccctgcc gcttgcggcg ccaggccgtc tcgcggatgt
     3361 cccggccggc gttcagcgcc acccggaaaa tccacgcctt caggttctgc acctggccga
     3421 cttcctcctg gtgacgccag cacttaatga acgcctcttg caaggcgtcg cgggcgtctt
     3481 cgaggttgcc gaccaggtag tacagcgtac ccagcaactc tgcctgatgc tggttgaagg
     3541 ccgcttcgag cagagcagcc ccgcgcgcac gggccttggc agccggctca ttcgctgcgg
     3601 ccttgcgggc gtgctgcgtc acactcatgc cttctgatga atagacggaa tattagcgct
     3661 gaaggattca accgccgcgc ctagttttta gcagaatctg gcgtaaagtg cgcaggggaa
     3721 agggtttggg gcggagtgcg aaatgcggaa tgccgagtga aggcagcggc ggactatacc
     3781 aagtttgcat ttagtcccgt atttgcgtcg cttgttcaag acgacttgtc tggcattccg
     3841 cgctcaaact ccgcattccg cactcgccga ctaccgagaa taatattccc catgtggccc
     3901 gaggcagaaa aaacgcagca gcttcttgcc gacgctaagg gcggcgatgc cgaggccgtc
     3961 aatcgcctga tggagcggca ccgcgagtcg ctgcggcgga tggtgcagct gcggctggat
     4021 cagaagattc agcggcggat cgatgtcagc gatgtcgtgc aggatgtgct ggtcgaagcc
     4081 aaccgccggc tgcaggatta tctcgccaac ccggtgattc cgtaccacct ctggctgcgg
     4141 cagatcgccc aggaccggat catcgacgcc caccgccgcc accgcgccag cgccaagcgt
     4201 tcggtcgata aggagcgggc cctcgccgtg ccggcagccg acgatcacag cacgatccag
     4261 ctcgccgccc agctttgcga tcggcagctc acccccgccg ccgcggctac gcaggccgag
     4321 ctggccaaag ccgtcgaagc ggccatcgcc aagttgcccg accaggactg cgaaatcatc
     4381 gtgatgcggc actacgagca gctgagcaat caggagatcg cccaggccct cggcctgacc
     4441 gaacccgccg ccagcatgcg ctatctgcgg gcgatccgcc ggctaaagga gctgatggtc
     4501 aatcctgaca gcgaagcgag cgagtcatcg tagaggagga accggcggga cgatgtccgt
     4561 ggcagggcgt cgcaccatga tccaatacat gtagaggagc aatagcacga tggggatcag
     4621 gccgatcggc aaggcgattc cgaggatcgt atggacgctc gccgctccct ccgacgtatt
     4681 gccgggttcg agtacagctc gggcaggatt cgcagggttg taataaaccg gatgagtcgt
     4741 cccgacggca tagttggcgg ctctggattg agctgaccct tcgtcccaca agttgt
//
LOCUS       DASRJB010000820         5578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2208901, whole genome shotgun sequence.
ACCESSION   DASRJB010000820 DASRJB010000000
VERSION     DASRJB010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208901"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFB80_21535"
     CDS             complement(<1..1244)
                     /locus_tag="VFB80_21535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36433.1"
                     /translation="MKRWRGVCVGAGYFSQFHYDAWRRMDDVEIVAVCDLDRAKAEQV
                     ASKYGIPVALGDPRSAFDQPDIDFVDIITPPASHLSLVQAAAGRVGAIICQKPLAPDL
                     QAAQALVAAARDRNCRLMVHENFRFQPWHREIRRLLDQGANGERLHTITCRSRPGDGW
                     AEDAYLSRQPYFRTMPRLLVHETGVHLIDVFRFLGGELDWAWADLRRLNSAIAGEDAG
                     LLTFRFQSGALAVWDANRYNESTAADPRYTFGEFLIEGSGGSIRLYPDGRLTIQRLGQ
                     QEVDHPYEHSHHGFGGDCVLATQRHFIDCLKTGQPFETGGEEYLKNLLAVEAVYQSAQ
                     TGNRVQIGGERGSETASRRRVVDLSLPIDERLPGAKVEVFKRLETDGWNATNITLYSH
                     CGTHMDAPRHFVPGGDPLEKMDL"
     gene            complement(1241..2500)
                     /locus_tag="VFB80_21540"
     CDS             complement(1241..2500)
                     /locus_tag="VFB80_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN36434.1"
                     /translation="MQRIGVRHVFVVATFLVSVLLYVDRACISTAKDAIARDLGLSDT
                     AWAWVMSAFALGYALFQTPTGMLSDRLGPRRVLAAVVVFWSLFTGLTAAAFNLASLLV
                     VRFLFGAGEAGAFPGMARAAYSWFPVQERGVIQGINFSGGRIGAALALPVMALAIDSL
                     GWQLSFTILMLLGFVWATGWYLSFRDDPAEHFLVSPDELAHIREHRQQAGAPPARQLQ
                     LGELAASPNVWLLCGQYFASNFTFFFCLTWLHPHLKETYRLTSSEAGVYSSAPFLCGA
                     AGNWLAGWIVDRIFRSGRWNASRRWPAEIGFCLAAVGVVGCAYARSPLTSVSWLSLAV
                     LGADMTLAPSWSVCIDIGRTSSGMVSGTMNMAGNLGSFLTSLAFPVLRQWYASPRPFF
                     FVAAGLSALAMLVWLFINPQRPVEARS"
     gene            2655..3719
                     /locus_tag="VFB80_21545"
     CDS             2655..3719
                     /locus_tag="VFB80_21545"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN36435.1"
                     /translation="MPGHSSRRGSAWLLAFAVASASAHAAEPAFPGKSWQQKSAAELG
                     LDPKRLEAVAAALGGRGCIVKDGFVVHQWGDQAQTGDWLSSAKPVLSTLLLFAVHEGK
                     LKSIDTPIAEFGWELSPKDRTMTLRHLANMTSGYARPEEPGKAWAYNDFAIQLYQKTL
                     FDRVFKDDPDKVANSPERLGALGLEDGLSFTPDRRRMKASVRDFARIAWFWMHRGRWQ
                     DRQLLPQRLFDENMRPQTPKDLPVSQPAETNDYLKIGSYGGGSEHFTRYGAGIYGCNW
                     WFNATGGSHPDKPTWPDAPADTVMSIGAGGNNSVLMPKLNLVVAAAQADWGKLEAGAS
                     DSVLNQRLQLIARAGSPAVQ"
     gene            complement(3729..3980)
                     /locus_tag="VFB80_21550"
     CDS             complement(3729..3980)
                     /locus_tag="VFB80_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36436.1"
                     /translation="MDDERKDGGGMAVFVAAGCLVVVVGLIAAVGGWVVIRSRFAAEQ
                     ELMLREDMQAELEQALAAQQEAIAKSQQEREAAQPAKNE"
     gene            4059..4904
                     /locus_tag="VFB80_21555"
     CDS             4059..4904
                     /locus_tag="VFB80_21555"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HZN36437.1"
                     /translation="MGRNKLELVREEKLFRLLPGRSEKSRLEASGVALLDDATALVVF
                     DNLNQVARIDLSLKRRRTNGLFPAPSLGAGFEDIAIDRRQGRFLCLVESVEDFDGIHR
                     SFVAEYDSEGHFLACTRLPSRLRKANKGFEGLAHEWLGSREYLYALSEGNVGPSGQRG
                     GQIEVFLRDASGWKASRRIRLPKQANFDDYAALAYRDGQVAIVSQESARLWVARLDRK
                     ARGVVPGSEAVYRFPSKSYGNVEGIAWLSRDTLVAVSDRRKRDQPERCSAKDQAIHVF
                     RIPAE"
     gene            complement(4877..5182)
                     /locus_tag="VFB80_21560"
     CDS             complement(4877..5182)
                     /locus_tag="VFB80_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36438.1"
                     /translation="MTIYMQGTGPLLVVILTAIALVLIGLLGLAAAIGSVKPWKAGLC
                     IGLVAVVFTGVLVQYGWSGGEFPRIVRRVRLRAVPPVRRDPAASARPAAHSAGMRNT"
     gene            5344..5417
                     /locus_tag="VFB80_21565"
     tRNA            5344..5417
                     /locus_tag="VFB80_21565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5378..5380,aa:Ile,seq:cat)
BASE COUNT         1028 a         1896 c         1786 g          868 t
ORIGIN      
        1 aggtccatct tttcgagcgg atcaccgccg ggcacgaaat gtcgcggggc gtccatgtgc
       61 gtgccgcagt gcgaatagag cgtgatgttc gtcgcgttcc agccgtcggt ctccagccgc
      121 ttgaagactt cgaccttggc ccccggcagc cgctcgtcga tgggcagcga gagatcgacg
      181 acgcggcggc gcgacgctgt ctccgatccc ctctccccgc cgatttgcac gcgatttccc
      241 gtctgcgccg actggtacac ggcctccacg gcgaggagat tctttaggta ttcctcgccg
      301 ccggtttcga acggctgccc cgttttcagg cagtcgatga agtgccgctg ggtggccagc
      361 acacagtcgc cgccgaaacc gtggtgcgaa tgctcgtacg gatgatcgac ttcctgctgc
      421 cccagccgct ggatggtgag gcggccgtcg ggatagagcc ggatcgagcc gccgctgcct
      481 tcgatcagga attcaccgaa cgtgtagcgc gggtccgcgg ccgtcgattc gttgtagcga
      541 ttggcatccc acacggccag cgcgccgctc tgaaagcgaa acgtcaacag ccccgcgtct
      601 tcgcccgcga tcgcgctgtt gagccgccgc aaatcggccc aggcccagtc cagctcgccc
      661 ccgagaaagc gaaacacgtc gatcaaatgc accccggtct cgtgcacgag cagccgcggc
      721 atcgtgcgaa agtagggctg ccggctgagg taggcgtcct ccgcccagcc gtcgccaggc
      781 cgcgagcggc aggtgatggt gtgcaaccgc tcgccgttcg ctccctgatc cagcaggcgg
      841 cgaatctcgc gatgccaggg ctgaaagcgg aagttttcgt gcaccatcag gcggcagttt
      901 cggtcgcggg ccgcggcgac aagcgcctgg gccgcctgca ggtcgggcgc cagcggcttc
      961 tggcagatga tcgcgccgac tcgcccggcc gcggcctgca ccaggctcag gtgagaagcc
     1021 ggcggcgtga tgatatcgac gaaatcgatg tccggctgat caaacgcgga ccgcgggtcg
     1081 cccaaggcaa caggaatgcc gtacttgctc gccacctgct cggctttggc gcgatcgagg
     1141 tcgcacacgg cgacgatctc gacgtcgtcc atccgccgcc aggcgtcgta gtggaactgg
     1201 ctgaagtagc cggctccgac gcagacgccg cgccagcgtt tcatgaccgg gcctccacgg
     1261 gccgctgcgg gttgatgaat agccacacga gcatcgcgag ggcgctcagc ccggcagcga
     1321 cgaagaagaa gggccgcggc gaagcgtacc actgccgtaa tacggggaac gccaggctcg
     1381 tcaaaaagga ccccaggttg cccgccatat tcatcgtgcc ggacaccatt cccgagctcg
     1441 tccgcccgat gtcgatgcac acggaccagg acggcgccag ggtcatgtcg gcgcccagta
     1501 cagccaggct gagccaactc acgctggtca gcggcgagcg ggcgtaggca cagcccacga
     1561 ctcccacggc tgcaaggcaa aagccgatct ccgcgggcca gcgccgcgac gcgttccatc
     1621 gtccggagcg gaagatgcga tcgacgatcc acccggcgag ccagtttccc gctgcgccgc
     1681 aaagaaaggg cgcggaagag taaactcccg cttcggacga agtcaaccgg tacgtttcct
     1741 tcaaatgagg gtgcagccac gtcagacaga agaagaacgt gaagttgctg gcaaaatact
     1801 gcccgcacaa gagccatacg ttcggcgagg cggccagctc gcccaattgc agctgccgcg
     1861 ctggcggggc gccggcttgc tgccgatgct cgcggatgtg cgccagctca tctggactga
     1921 cgaggaagtg ctccgcggga tcgtcgcgaa acgacaggta ccagcccgtc gcccagacga
     1981 agcccagcag catcaggatc gtgaagctga gctgccagcc gagtgaatcg atggccagcg
     2041 ccatcacggg caaggccaac gccgcgccga tccgcccgcc cgagaagttg atcccttgaa
     2101 tgacgccgcg ctcctggacg ggaaaccagg agtaggccgc gcgggccatt cccgggaagg
     2161 ccccggcctc gccggcgccg aacagaaacc gcaccacgag cagcgacgcc agattgaagg
     2221 cggccgcggt cagcccggtg aacagcgacc agaaaacgac cacggccgcc agcacgcgcc
     2281 gcgggcccag acggtcggag agcattccgg tcggagtctg gaagagggca tagcccagcg
     2341 cgaaggccga catgacccag gcccaggccg tgtcgctcag acccagatct cgggcgatgg
     2401 cgtctttcgc cgtcgagata caggcccgat cgacgtagag cagcaccgac acgaggaacg
     2461 tggcgacgac gaagacgtgg cgaacgccga tccgctgcat ggcagcaggc actgtaccgc
     2521 agcggcgtcg gcggcaccat gacttcggct ccccccgaat cgttcgccat tacttgattg
     2581 gtggcgaagt tccggcccgt tatcatcagc cctattcact gccgggaacc gttctgtttg
     2641 acgaaggacc agccatgcct ggccactcct ctcgccgcgg ctccgcctgg ctgctcgcat
     2701 tcgccgtcgc atcggcttcc gcgcacgcgg ctgagccggc ctttcccggg aaaagctggc
     2761 agcaaaagtc tgcggcagag ctgggcctcg atccaaaacg gctcgaggcc gtggccgcgg
     2821 ctctgggcgg acgcggctgc atcgtcaaag acggcttcgt cgtccaccag tggggcgacc
     2881 aggcgcagac cggcgattgg ctgtcgagcg ccaagccggt gctttcgacg ctgctgctgt
     2941 tcgccgtcca cgaaggcaag ctgaaatcga tcgacacccc gatcgccgag ttcggctggg
     3001 agctttcgcc caaggaccgg acgatgaccc ttcgccacct ggccaacatg accagcggct
     3061 acgcccgccc ggaagagccc ggaaaggcct gggcctacaa cgacttcgcg atccagctct
     3121 atcagaagac gctgttcgac cgcgtgttca aggacgatcc cgacaaggtg gccaatagtc
     3181 ccgagcggct gggcgccctg ggattggagg acggcctgtc gttcacgccc gaccgccggc
     3241 ggatgaaggc gtcggtacgc gacttcgccc gcatcgcctg gttctggatg caccgcggcc
     3301 gctggcaaga ccggcagctg ctcccgcagc gcctgttcga cgagaacatg cggccgcaga
     3361 cgcccaagga cttgcccgtt tcgcagccgg ccgagaccaa cgactatctc aagatcgggt
     3421 cctacggcgg cggctccgaa cacttcacgc gctatggggc gggcatctac ggctgcaact
     3481 ggtggttcaa cgcgaccggc ggctcccatc ccgataagcc cacctggccc gacgcgccgg
     3541 ccgacaccgt gatgagcatc ggcgctggcg gcaacaactc ggttctgatg ccgaagctca
     3601 acctcgtcgt cgccgccgcg caggccgatt ggggcaagct ggaggccggc gcgagcgact
     3661 cggtcctcaa tcagcgcctc cagctgatcg cccgggcagg atcaccggcg gtccagtagg
     3721 cagcgagttc actcgttctt cgcgggttgg gcggcttcgc gctcttgctg ggacttggcg
     3781 atcgcctcct gctgcgccgc aagggcctgc tctagttcgg cttgcatgtc ctcacgcagc
     3841 atcagttcct gctccgcagc gaaccgagac cgaatcacga cccatccacc caccgccgca
     3901 atcaacccca ccacgacgac caggcacccg gcggcgacga aaaccgccat cccgccgccg
     3961 tcttttcgct cgtcgtccat ggttcccatt ctagccgggc gcgagcggca acgccgcatc
     4021 ggccacggag tatactggcg gccgctgagg ttcactccat gggccggaac aaactggaac
     4081 tggtccgcga ggagaagctg ttccgcctgc tcccaggtcg ctcggagaag agccggctgg
     4141 aggccagcgg cgtggcactg ctcgacgatg cgacggcgct cgtcgtgttc gacaacctca
     4201 atcaagtcgc tcggatcgac ttgtcgctga agcggcggcg gaccaacggg ctcttccccg
     4261 caccgagcct gggggccggc ttcgaggaca tcgccatcga ccggcggcag ggccgcttcc
     4321 tctgcctggt cgaatccgtc gaggattttg acggcatcca ccgcagcttc gtcgccgagt
     4381 acgactccga ggggcacttt ctggcgtgca cgcggttgcc gtcgcggctg cgaaaggcca
     4441 acaagggatt cgagggcctg gcccacgagt ggctgggcag ccgcgagtac ctgtacgccc
     4501 tgtcggaggg caacgtgggc ccatccggcc agcgcggcgg ccagatcgaa gtgtttctgc
     4561 gcgacgcttc cggctggaag gcgtcgcgcc ggatccgctt gccgaagcag gcgaacttcg
     4621 acgactacgc ggcgctggca tatcgcgacg gacaggtggc gatcgtctcg caggagtcgg
     4681 cccggctgtg ggtcgcccgg ctggaccgca aggcccgcgg cgtcgtgccg ggatcggagg
     4741 ccgtctatcg ctttcccagc aagagctacg gcaacgtcga agggatcgcc tggctctccc
     4801 gcgacacgct ggtcgccgtc tccgatcgca ggaaaaggga ccagccagag cgctgctccg
     4861 caaaggatca ggccattcac gtgttccgca tcccggccga atgagccgcg ggccgggccg
     4921 acgccgccgg atcgcggcgg acaggtggaa ctgctcgcag ccggacgcgg cgcacaatgc
     4981 gcggaaactc accgccgctc catccgtact gcaccaaaac gccggtgaac acgacggcca
     5041 ccagcccgat gcacagcccg gccttccacg gcttgacgct gccaatcgcg gccgccagcc
     5101 ccagcaggcc gatgagcacc agcgcgatcg cggtgaggat tacaaccagc agcggaccgg
     5161 ttccctgcat gtagatagtc atggatcgtg ctctccggat aactggccct ggagagccta
     5221 tccacaaacg ccggccccgg ccgacagcga attccggcag aaccatcgct tctgccgccg
     5281 tgtccgtggg aacatcgcaa ggtttcggcg gaatcgcacc gccggtagaa tcggttgcac
     5341 agcggggccg tagctcaatc ggtcagagca gaggactcat aatcctttgg ttcccggttc
     5401 gagtccgggc ggccctacct tacacagcaa cgacttgcgg cgattctgag gatcccgaaa
     5461 tcgccaagcc acgttgtttg cacgtaactt tcaattccag gagcttgcta atgcctgctg
     5521 acgatctctc cgcatttccc gacccgaaga accgcggtat gacaattcgc gagtggtg
//
LOCUS       DASRJB010000821         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3906398, whole genome shotgun sequence.
ACCESSION   DASRJB010000821 DASRJB010000000
VERSION     DASRJB010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906398"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="larB"
                     /locus_tag="VFB80_21570"
     CDS             <1..378
                     /gene="larB"
                     /locus_tag="VFB80_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZN36439.1"
                     /translation="AGTSDMPVAEEVRETADWMGAETELIVDVGVAGPQRLLAKLDRI
                     TTADAVVVVAGMEAALASVVGGHVACPVIAVPTSVGYGASLGGIAALLGMLNSCAANV
                     AVVNIDAGFKAGYLAGLIARRRC"
     gene            522..1049
                     /locus_tag="VFB80_21575"
     CDS             522..1049
                     /locus_tag="VFB80_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36440.1"
                     /translation="MPQPLKRCRLTVLAVLAISSGLVGCSAFQERPTPQLNSQLTPPD
                     GPVVQPAAKYVVEIRSAKGKSQAVEKDLSEPIHVQAALEKTGAHKKYRRMDVELYRPL
                     PNGGWHKMQLEFDRANKQVPPEYDYSLLPGDRVIVTEDPRTIIDDTLEFVLQPIGIDP
                     PNRKAKLSDKYEIRG"
     gene            1132..2454
                     /locus_tag="VFB80_21580"
     CDS             1132..2454
                     /locus_tag="VFB80_21580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN36441.1"
                     /translation="MNARVASLTALLTVVACQVLAAADPPAGWTTAAPREELRPTFSF
                     QPSGGRGGRECFVIAGDEREGLSGWWTRTFEVEGGKTYRFVAWRKGEGALSLRRSGVA
                     RVTWRDAAGKPVRHDEETQFGYQAGRKPQAEPEHPLDKSIDAAGWTEVSDTYLVPQAA
                     RQAVVELHYRWSKGGRIAWSDVSLEPVIYQPRKVRLATIHYQPRSGKTPAAKREQFAP
                     LIAKAAAQKADLVVLPETLTYYQTGLSYTECAEPIPGPSTDYFAQLAKKHDLYLVAGL
                     LEREGHLVYNVAVLIGPEGVVGKYRKVCLPRSEIDGGVQPGSEYPVFDTRLGKIGMMV
                     CYDGFFPEVARELTNRGAEVIAFPVWGCNPLLASARACENGVYVVSSTYSEPSQNWMP
                     SAIFGHAGERLAEGKEWGDVVVAEVDLNRRMHWSSLGDFKAEVQRHRP"
     gene            complement(2559..2780)
                     /locus_tag="VFB80_21585"
     CDS             complement(2559..2780)
                     /locus_tag="VFB80_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36442.1"
                     /translation="MTPTATVLHEQGYNSGMAAAGSPNVLDQILDPLIEAFTPEVARR
                     IAAITLDAKTQVAQRRIGGEGKHGSIDSD"
     gene            complement(2801..3376)
                     /locus_tag="VFB80_21590"
     CDS             complement(2801..3376)
                     /locus_tag="VFB80_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZN36443.1"
                     /translation="MTSDKDAHDDSPHEIAICGDLTEHESDVSEKLLGVPLGGECVLY
                     FNSPGGSAYVALSLASLIVLRGLNATGVVLGECSSAAIWPLAACRRRIVTAHSVLLFH
                     PLKWESGEHVDIAEAAEWARHFATLEQEMDAMLARLLGVPIERLAAWLKPGRYVTGRE
                     LAEAGLAELVELAPRTDLALRPAAKRKPRRP"
     gene            3563..>3763
                     /locus_tag="VFB80_21595"
     CDS             3563..>3763
                     /locus_tag="VFB80_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36444.1"
                     /translation="MGIKFHCPQGHKLNVKSFLAGKKGVCPKCGTRVRIPTASEPGLG
                     DADDDEEAGGSAAALPGNGSAKA"
BASE COUNT          655 a         1157 c         1310 g          641 t
ORIGIN      
        1 gcgggaacga gtgacatgcc ggtcgccgag gaggttcgcg aaacggccga ttggatgggg
       61 gccgaaacgg agttgatcgt cgatgtgggt gtcgccgggc cgcagcgatt gctggccaag
      121 ctggaccgca tcacgacggc cgacgcggtc gtcgtcgtgg ccggaatgga ggccgcgctg
      181 gccagtgtcg ttggcgggca cgtggcctgt cctgtgatcg ccgtgcccac gagcgtcggt
      241 tacggcgcga gtctcggcgg catcgcagcg ctgctgggca tgctcaacag ctgcgccgcc
      301 aatgtcgccg tcgtgaacat cgacgctgga ttcaaggccg gctatctcgc cgggctgatt
      361 gctcgccgcc gctgctagcg gctcgcggat cgcgattctg ccggattctc taaagaccgc
      421 cgcgggccct gccgatattg ggccgcgagc gaccagattg gccgcgaaac gttttgccag
      481 caaagccgac tcggatgcct gttagcaaag ggatttgcac catgccccag cccttgaagc
      541 gatgccgcct gacggtgctg gcagtgctgg caatttccag cgggctggtc ggctgctcgg
      601 cgttccagga gcggccgacg ccgcagctca attcgcagct tacgcccccc gacggcccgg
      661 tcgtgcagcc ggcggccaag tacgtggtgg aaattcgctc ggccaagggc aagtcgcaag
      721 cggtggagaa ggacctgtcg gagccgatcc acgtgcaggc ggccctcgag aagaccggcg
      781 cgcacaagaa gtatcgccgg atggacgtgg agctctaccg cccgctgccc aacggcggct
      841 ggcacaagat gcagctcgag ttcgatcgcg ccaataagca agttcctccc gagtacgact
      901 actcgctgct gcccggcgac cgcgtgatcg tgaccgaaga tccgcggacg atcatcgatg
      961 acaccctcga attcgtcttg cagccgatcg gcatcgatcc gcccaaccgg aaggcgaagc
     1021 tctcggacaa gtacgagatt cgggggtagc cccggcccct gaaaagtgcg aagctgagca
     1081 gccgagcggg tgctcgtggc tgccaggttt tgcacttcgg ggggaccgat gatgaacgct
     1141 cgcgtcgctt cgctgacggc attgttgacg gtcgtggcgt gtcaggtgct cgcggcagcc
     1201 gatccgccgg cgggctggac gacagctgcg ccgcgcgagg aattgcggcc gacattcagt
     1261 ttccagccct cgggcggccg cggcggacgg gaatgctttg tcatcgcggg ggacgagcgc
     1321 gaaggtctca gcggctggtg gaccaggacg ttcgaggtcg aaggcggcaa gacctatcgt
     1381 ttcgtggcgt ggcggaaagg cgagggcgcc ctgtcgctgc gccgctcggg cgtcgcccgt
     1441 gtgacgtggc gcgacgcggc ggggaagccc gtgcggcatg atgaagagac tcagttcggc
     1501 taccaggccg gccgaaagcc gcaagcagag ccggaacatc cgcttgacaa aagcatcgac
     1561 gccgccggct ggaccgaagt gtccgacacg tatctggtcc cgcaggcggc ccggcaggct
     1621 gtggtcgagc tgcactatcg ctggtcgaag ggcgggcgga ttgcctggag cgatgtctcg
     1681 ctggagccgg tcatttatca gccgcggaag gtgcggctgg caacgatcca ctatcagccg
     1741 cgatcaggca agactccggc ggccaagcgc gagcagttcg cgccgctgat cgccaaggcg
     1801 gcggcacaga aggcggacct ggtcgtgctg ccggagacgc tgacgtatta ccagacgggc
     1861 ctctcctaca ccgaatgcgc cgagccgatc cccggtccct cgaccgatta ctttgctcaa
     1921 ctggcgaaga agcacgacct ctatctcgtg gcggggttgc tcgagcggga ggggcacctg
     1981 gtctacaacg tcgcggtgct gatcgggccg gagggcgtcg tcggcaagta tcgcaaggtc
     2041 tgcctgccgc ggagcgagat cgacggcggc gtgcagccgg gcagcgagta tccggtgttc
     2101 gacacgcggc tgggcaagat cggcatgatg gtctgttacg acggcttctt cccggaagtg
     2161 gcccgcgaat tgaccaaccg cggcgccgaa gtgatcgcgt tcccggtctg gggctgcaat
     2221 ccgcttttgg cttccgcgcg ggcctgcgag aacggcgtgt acgtggtcag cagcacctac
     2281 agcgagccct cgcagaattg gatgccgtcg gccatcttcg gccatgccgg tgagcggttg
     2341 gccgagggca aggaatgggg cgacgtcgtc gtcgccgagg tcgatctcaa ccgccgcatg
     2401 cactggtcca gcctcggcga cttcaaggcc gaggtccagc ggcaccggcc gtagcataag
     2461 cgtcctcgct tgtgcagcgc tgctgagagt ggaggcgtga gcaagcgaga cgcttgctct
     2521 acgggcgatc ttgccgcgac tcctcgtagt gttcccgctc agtcgctgtc aattgacccg
     2581 tgtttgcctt caccgccaat tcgtcgctgc gcgacttgag tcttggcatc caaagtgatc
     2641 gcggcgattc gccgagcgac ttccggcgtg aaggcctcga taagtggatc gaggatctga
     2701 tcgagaacgt tgggtgagcc agcggccgcc atgcctgaat tgtatccttg ctcgtgcagc
     2761 accgtggcgg ttggagtcat gagcaagcga gacgcttgct ctacgggcga cgcggcttgc
     2821 gtttggccgc cgggcgtagg gcgaggtcag tgcgcggggc gagctcgacg agctcggcca
     2881 ggccggcttc ggccagctcg cggccggtga cgtagcggcc gggcttgagc caggcggcga
     2941 gccgctcgat gggcacgccc aacaagcggg ccagcatggc gtccatctcc tgctcaagcg
     3001 tggcgaagtg gcgggcccat tcggcggctt cggcgatatc aacgtgctcg ccgctttccc
     3061 acttgagcgg gtgaaacagc agaacgctgt gagcggtcac gatccgccgg cggcaggcgg
     3121 ccagcggcca gatcgccgcc gaggagcatt cgcccagcac gacgccggtc gcgttcaatc
     3181 cgcggagcac gatcagcgac gccagcgaca gcgcgacgta cgcgctgccg cccggagaat
     3241 tgaaatagag cacgcactcg ccgcccagcg gcacgcccag cagcttctcg ctgacgtcgc
     3301 tctcgtgctc ggtcaaatcg ccgcagatgg cgatttcgtg gggggagtcg tcgtgggcgt
     3361 ctttgtcact tgtcattggt cacttgtcag tagtcagtgg ttagttgacg gaggcagtcg
     3421 gccgaccaac tgaccactga caactggcca ctgaccaaat atgccgattc tgcgggccgc
     3481 cgaatggtcg cgaaatgacg aaccgcgtgt ataataagga gctggctttg tccagagggc
     3541 agccgcgttt ttcactggtc cgatgggcat caagtttcac tgtccgcagg gacacaagct
     3601 taacgtcaag tcgtttctgg ccggcaagaa gggcgtctgc cccaaatgcg ggaccagggt
     3661 ccgcattccc accgccagcg agcccggact gggggacgcc gacgacgacg aggaagcagg
     3721 cgggtccgcg gcggcactgc cgggcaacgg ttcggccaaa gcg
//
LOCUS       DASRJB010000822         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_530716, whole genome shotgun sequence.
ACCESSION   DASRJB010000822 DASRJB010000000
VERSION     DASRJB010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530716"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..2720
                     /locus_tag="VFB80_21600"
     CDS             192..2720
                     /locus_tag="VFB80_21600"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZN36445.1"
                     /translation="MKLSLGIALSFCSLISLAAAPAALAQRGGPFGTRGAGPGGGGAG
                     GGSEIRPYDDVITKEAKSKPGVFLTHQIGEKLFYEIPSDKLGLDMLWVWQLGRTQTGF
                     GYAGSPLGDRTVRWELRGEQVLLRDVKYAIRADKKGAIQQAVESTSVAPIVKAFPVRA
                     WGKDKAPVIEVTSLFTSDVSEISARRELNAGTLDTSRTYFEEIKAFPRNLEVKVLATY
                     SLGGGGGGERGGPSPSPTPTPTPGRRGGGRDPSQSAVTVVVHHSMLLLPEQPMKPRRK
                     DERVGFFDVGFEDYADDSHHQVENVRFINRWRLEKKDPAADVSEPKQPIVFYVGREVP
                     EKWQDYVKRGIEMWQPAFEAAGFKNAIVAKPAPTPREDPNWDAEDARISTVRWLPATV
                     ENAFGPHVHDPRTGEILEADVRMYHNVLKLARDWYFVQASPSDPRAQKLPLPDDLIGE
                     LLAYIVAHEIGHTLGFPHNMKASAAFTVKQLRDAEFTKKWGTEASIMDYGRFNYVAQP
                     GDGAALIPKIGPYDFFAIEWGYREFKNDEEEKKGLAALVAKQKTDGQLRFGDAQPSED
                     PSRQTEDLGADPIEATELGLKNLQRVGLYLVDACCKPGEDYDLLRNMYAQLIAQRDRE
                     LRHVTGMIGGYEQINLYFGDADRVYHTIPADRQREAVKFLLTHAFQTPAELVDPAITL
                     RLEAGGAADRILSSQSSILATLLSETRFKRMAEQVQRAKAGEATYAPWELLTNLRTGI
                     WSELDQERVTIDLYRRNLQRAHVARLASLLDSGSDSDLAAFSRSELNALKDQIQKKLE
                     AADPVAQVHLNEVLAEIARAFDPASRPAAATPTPTGGPFPRPSP"
     gene            complement(2734..4065)
                     /locus_tag="VFB80_21605"
     CDS             complement(2734..4065)
                     /locus_tag="VFB80_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN36446.1"
                     /translation="MRHPFRFAGWLVVAFQTLAAAVLADDWPQWMGLNRDDVWRETGI
                     VEKFPSGGPKILWRTPIAGGYSGPAVAGGKVFVTDFVRESGEAKNDPGARPELKGKER
                     VLCLNARDGAIVWKYEYDCPYQISYPAGPRATPTVSGGKVYVLGAEGNLICLDAEKGN
                     VVWSKELKKEYKIEAPMWGFTGHPLVDGNKLICLVGGKGSVAVAFDKDSGKELWRALT
                     ATEPGYCPPSIIDHGGKRQLLIWHPEALNSLNPETGEIYWSEPLKPQYGMSITAPRKS
                     GDFLFAGGIGNCAALFKLDAAKPAATVVWDGTPTTAVYCGTSTPAIEDGVIYGNDCQV
                     GNLRAVKLETGERLWETFQPTTGGDRRASHGTAFLTKNGDRYFLFSETGDLVIARLTP
                     AKYEEISRAHLLEPTGECFGRAVVWSHPAYANKCVFARNDKEILCASLAKE"
     gene            4302..>4849
                     /locus_tag="VFB80_21610"
     CDS             4302..>4849
                     /locus_tag="VFB80_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36447.1"
                     /translation="MAERFLGQRAIVLGGSMGGLLAARVLADHFREVVVLERDPLPDQ
                     PAARKGTPHARHTHGLLSEGLAKLEAMFPGFSTELVHHGAEQGDLVKDCLWHAAGGFH
                     RQIASGLFASAQSRALLEWLVRRRVSNISNVTLKGEIGVQRLLADGQHRRITGVQYAD
                     RSYGGGLLDPGAAFKSDRTEALS"
BASE COUNT          850 a         1612 c         1575 g          812 t
ORIGIN      
        1 atttcggcat cgtcgcctcc gccctggaga ggccgaaagt ctatcgatgc tggcacgttt
       61 tgaggaacac caatctggca gtcggcgcta agtggaacag tgtgcgcctt gctgctggcg
      121 gaccgtccgg cgagcggcta cgctggaagc ccatctgcgg gcaatttctg ccgttcttcg
      181 aggccactct catgaagctc tccctgggaa ttgcattgtc gttttgctct ttgattagtc
      241 tggctgccgc gcctgccgca ctcgcgcagc ggggcgggcc gtttggaacg cgcggcgcgg
      301 ggccgggcgg cggcggcgcg ggtggcggca gtgagattcg gccgtacgac gacgtgatca
      361 ccaaggaggc caagagcaag ccgggagtgt tcctcacgca tcagatcggc gaaaagctgt
      421 tctacgagat tccctccgac aagctcggcc tggacatgct ctgggtctgg cagctcggcc
      481 gcacgcagac gggcttcggc tacgccggct cgccgctggg cgaccgcacc gttcgctggg
      541 agctgcgggg cgagcaggtg ctcttgcggg acgtgaagta cgcgatccgc gccgataaga
      601 agggcgcgat ccagcaagcg gtcgaatcga ccagcgtcgc gccgatcgtg aaggcgtttc
      661 cggtgcgggc ctggggcaaa gacaaggctc cggtcatcga agtcacgtcg ctcttcacct
      721 cggacgtctc cgagatcagc gcccgccgcg agctcaacgc cgggacgctc gacacctcgc
      781 ggacctactt cgaagagatc aaggcgtttc cgcggaacct ggaagtcaag gtgctggcga
      841 cctattcgct cggcggcgga ggcggcggcg aacgcggcgg gccgtcgccc agccccacgc
      901 cgacgcccac gcccggtcgc cgcggcggcg gacgcgatcc ttcgcaatcg gcggtcaccg
      961 tcgtcgttca ccacagcatg ctgctcttgc cggaacagcc gatgaagccc cgccgcaagg
     1021 acgaacgcgt cgggttcttc gacgtcggct tcgaggacta cgccgacgac tcgcaccacc
     1081 aggtcgagaa cgtgcggttc atcaaccgct ggcggctgga gaagaaggac ccggcggcgg
     1141 acgtttccga gcccaagcag ccgatcgtgt tctacgtcgg ccgcgaagtg ccggagaagt
     1201 ggcaggacta cgtgaagcgc ggcatcgaga tgtggcaacc ggcgttcgag gcggccggct
     1261 tcaagaacgc gattgtggcc aagcccgcgc cgaccccgcg cgaggacccg aactgggacg
     1321 ccgaggatgc gcggatttcg acggtccgct ggctgccggc gacggtcgag aacgcgttcg
     1381 gcccgcacgt gcacgatccg cggacgggcg aaatcctgga ggccgacgtc cgcatgtacc
     1441 acaacgtgct gaagctggcc cgcgactggt acttcgtgca ggcttcgccc agcgacccgc
     1501 gggcccagaa gctgccgctg ccggacgacc tgatcggcga gctcttggcc tacatcgtgg
     1561 cccacgagat cgggcacacg ctcggcttcc cgcacaacat gaaggcctcg gcggcgttca
     1621 cggtcaagca gctccgcgat gccgagttca cgaagaaatg gggcaccgag gcctcgatca
     1681 tggactacgg ccgcttcaat tacgtcgccc agcccggcga cggggccgcg ctcattccga
     1741 agatcgggcc ctacgacttt tttgcgatcg aatggggcta tcgcgagttc aagaacgatg
     1801 aagaagagaa gaaaggcctg gcggcgctgg tcgcgaagca aaagaccgac ggccagctgc
     1861 ggttcggcga tgcccagccg tcggaagatc cttcgcggca gaccgaggat ctgggcgctg
     1921 acccgatcga agccaccgag ctaggcctca agaacctgca gcgggtcggc ctctacctgg
     1981 tggatgcctg ctgcaagccg ggcgaggact acgacctctt gcggaacatg tatgcccagc
     2041 tgatcgcgca gcgcgaccgc gagctgcgac atgtgaccgg catgatcggc ggctacgaac
     2101 agatcaacct ctatttcggc gacgcggacc gcgtctatca cacgattccc gccgaccggc
     2161 agcgcgaggc cgtcaagttc ctgctgacgc acgcgtttca gacgccggcc gagctggtcg
     2221 atccggcgat cacgcttcgc ctggaggccg gcggcgcggc cgatcgcatt ctctccagcc
     2281 agtcgtcgat cctggcgacg ctgctcagcg agacgcggtt caagcggatg gccgagcaag
     2341 tgcagcgggc caaggccggc gaggcgacct atgctccctg ggagctgctg acgaacctgc
     2401 ggaccggcat ctggtcggag ctcgatcagg agcgggtcac catcgacctc taccgccgga
     2461 acttgcagcg agcccacgtc gcgcggctgg ccagcctgct cgattcgggc agcgactcgg
     2521 acctggccgc gttcagccgc agcgagctga acgccctcaa ggaccagatt cagaagaagc
     2581 tggaggccgc cgaccccgtg gcccaggtgc acttgaacga agtgctggcc gaaatcgccc
     2641 gcgccttcga tccggcaagt cgcccggcgg cggcgacgcc gacgcccacc ggcggaccgt
     2701 tcccgcggcc atcgccatag gcaggccgca gccctactcc ttagccaggc tggcgcagag
     2761 gatctccttg tcgttgcgcg cgaagacgca cttattcgcg taggccgggt ggctccacac
     2821 gaccgcccgg ccgaaacatt cgccggtcgg ctccagcagg tgcgcccggc tgatttcctc
     2881 atacttcgcg ggcgtcaggc gggcgattac cagatcgcca gtctcgctga acaaaaaata
     2941 gcggtcgccg ttcttcgtca ggaaggccgt gccgtgcgaa gcgcggcggt cgccgccggt
     3001 cgtcggctga aaggtctccc acaatcgctc gccggtctcg agtttgaccg cccgcaaatt
     3061 tccgacctgg cagtcgttgc cgtagataac gccgtcctcg atggccggcg tgctggtacc
     3121 gcaatagacg gcggtcgtcg gcgtgccgtc ccagacgacg gtcgcggcgg gcttggccgc
     3181 gtcgagcttg aatagcgcgg cgcagttgcc gatgccgccg gcgaacagaa aatcgccaga
     3241 cttccgcggc gcggtgatcg acattccgta ctgtggcttc agcggctccg accaatagat
     3301 ctcgcccgtc tcgggattca ggctgttcag cgcctcggga tgccagatca gcagctgccg
     3361 cttgccgccg tgatcgatga tcgacggcgg gcaatagccg ggctcggtgg cagtgagcgc
     3421 ccgccacaac tccttgccgc tatctttgtc gaaggccacc gcgactgagc ccttcccgcc
     3481 gacaaggcaa atcagcttgt tgccatcgac gagcggatgc cccgtgaagc cccacatcgg
     3541 ggcttcgatc ttgtactcct ttttcagttc cttcgaccag acgacattgc ctttctccgc
     3601 gtccaggcaa atcaagttgc cttccgcacc gaggacatag accttgccgc cgctgaccgt
     3661 cggcgtcgcc cgcggaccgg cgggatacga aatctggtag gggcagtcgt actcgtactt
     3721 ccagacgatc gcgccgtcgc gagcattcag gcagaggact cgctccttac ctttcaactc
     3781 cggccgcgcc cccgggtcgt tcttcgcctc gccgctttcg cggacgaaat cggtgacaaa
     3841 gaccttgccg ccggcgaccg cggggccgct gtagccgccg gcgatcggcg tccgccacag
     3901 gattttcggg ccgccgctgg gaaatttctc gacgatgccc gtttctcgcc agacatcatc
     3961 ccggttcagt cccatccact gcggccaatc gtcggcgagc accgctgctg ccagcgtctg
     4021 aaaggccacg accagccaac cggcgaatcg aaagggatgt ctcatgccgg actcctgaaa
     4081 ttgcagggca atggcccaat taaggcgctt ccgaacttct gcggcaagtc ctcgaacggc
     4141 gcggctcgcg tcaagtcgtt gccggccaac agcttaggca ggcaaacgca agttttcgct
     4201 ccagctggtc acaaaaatcc gcgctcgggc tgaaacaaat ccgcggcggt tgcgccttcc
     4261 ttttttacac gggtttgtcc tcggaacagg aacaggcaga catggcggag cgttttttgg
     4321 gccagcgggc gattgtgctc ggtggcagca tgggcgggct attggcggcc cgcgttctgg
     4381 ccgatcattt tcgcgaagtc gtggtgctgg agcgcgatcc cttgccggat cagccggcgg
     4441 cccgcaaggg cacgccccac gcccggcata cgcacggcct gctgtcagag gggctggcga
     4501 agctcgaggc gatgttcccc ggcttcagca ccgagctggt ccaccacggc gcagagcagg
     4561 gagacctcgt gaaagactgc ctttggcacg cagccggggg attccaccgg cagatcgcca
     4621 gcggcctctt cgcctcggcc cagagccggg ccctgctgga gtggctcgtg cggcggcgcg
     4681 tcagcaacat ttccaacgtc acgttgaagg gcgagatcgg cgtccagcgg ctcctcgccg
     4741 acggccagca ccgccgcatc accggcgtgc agtacgccga tcgctcgtat ggcggcggcc
     4801 tgcttgaccc gggcgcggca ttcaaatcgg accgtaccga agccctctc
//
LOCUS       DASRJB010000823        10651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3617703, whole genome shotgun sequence.
ACCESSION   DASRJB010000823 DASRJB010000000
VERSION     DASRJB010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617703"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VFB80_21615"
     CDS             complement(<1..1411)
                     /locus_tag="VFB80_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36448.1"
                     /translation="MRRTSPNGRHWTLMVLAPLALLLFGGDAGQAQLKPPPSPSAPTT
                     TRPRDADDDSAKDPSESLFPGGAALKTDPEQQRLLKRADLCVADGRLDLAAVLWQKVL
                     DEAGDTLMLQHSQSSPVPVAIYTSLAQQVERTLAKLPPLALATYRTSADAEAQALLAA
                     AGPSGEEDALAAVVRRYFLSSVGDDAAYKLACLALDRYDFVGASRLLNKILDSHPDPS
                     IPRSELLLRLAVASARMGDRQSAEQALTKLTSAGGPRPSSDVVDLIVADVRSAAATSG
                     VGPAAKDWHMALGNPGRTGFMPALPEAVTSRTLSELWVREDPLALATLPGQQQYFGGG
                     AFMGGVVVMRGGMAMSQGGQVQQQAPVSREDLITNWRAGGWKPAGRLLFDGGRVYLKT
                     PDRLVCYSSVAHSQQPVWQSAWENRFELDSMSQMLAQMAMAYGYMPQQASSTRPKSPA
                     EVLLFADRVHQSMAIDGGVI"
     gene            complement(1453..3753)
                     /locus_tag="VFB80_21620"
     CDS             complement(1453..3753)
                     /locus_tag="VFB80_21620"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN36449.1"
                     /translation="MPLIAQIESLASWLVNPWLFVAGAAAILIPILIHLLNKRKFKIV
                     DWAAMEFLLDADKKNRRRIRLENLILLLLRCLAVFLIGMLLARPFIPTSVTAGLLNAA
                     QFERIVLLDDSLSMQARLGNEAAFEVARKRLSDLAGSLAQDQADNSFTLLLTSQPDRP
                     LFSATHLGTDNVSDIQASIEKLEPGDGTARLDASLAELEQYLASQPPNINRVVYVLSD
                     LRRRDWKEGDANPEAPLKILGRVAKLVQGCFLIDAGEDEDRNLTVSEIRPEGTLVQGV
                     QSRFDVAVTNNGTTAASDIRVKLSAGDSLPVETTIDQLAPGQTTSVRFNFTFSGDEEA
                     ESAERPLPPRQVKVELTTAQQGADDRLPADSVAYFPARLVRGIPTLIIDGDPSADFGK
                     AESFYLRRALAPIGPVPSGVAATIVTENELESLALDKFQVIFLLNNYRLGDKTAENIQ
                     RLEKWVEGGGGLVLMPGDQIDEQFFNDQYWRSGAGLSPVKLENIRGDESEKTWASLKV
                     EDASHEALKQFAGQNNPLLESVKVFRWWGASVKKEQLGKEVSIVAKLNDLEDSPAIVE
                     KPLGKGRVVAFTIPADADWHNWTSDPSYLLVVQDFVRYLAADRGSKGLVTVGQPLEQW
                     IDLTQYELDAPLVGPKELKANVQAAPPAGPGADQQTIWRMEFPAAASQGFYELKLARR
                     DGASDSVLFAANIDPPEGDLKRADRETMQKELAGTNVEIVSAKAAQSLADASTQTEIW
                     WYLLWGVVAVLAGEQLLGWFFGRSRT"
     gene            complement(3836..4741)
                     /locus_tag="VFB80_21625"
     CDS             complement(3836..4741)
                     /locus_tag="VFB80_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN36450.1"
                     /translation="MALPQYLDPKTLDRIKRLDVRARLVVEGFITGQHRSPYHGFAVE
                     FATHREYSPGDDTRHIDWKVWSRSDRLYIKEYEEETNLKCTILLDASKSMRYGEGWRK
                     YDYAATAAASLAYLLQQQQDAVGLVTFTTKVQKNLPASSHPNHLKLLFHELEQTTPDD
                     KTDVAQVFPELARQIRRRGMIVLLSDLFLPISTLAESLKQFRLRRHEVIVFQVMHDDE
                     LTFPFQDNTLFRGLEEPIQLHTEPRALRRGYLDAVERFLGDVRKTCASAGVDYVLMNM
                     KEPLDAVLGSYLTFRQKIRRTARHH"
     gene            complement(4816..5871)
                     /locus_tag="VFB80_21630"
     CDS             complement(4816..5871)
                     /locus_tag="VFB80_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN36451.1"
                     /translation="MIGTVQPSSDDLQALENLKSAYASVRKELAKVIVGQDEVIDQLL
                     IAIFAQGHCLLEGVPGLAKTLMVSTLARSLKLQFSRIQFTPDLMPSDITGTEVIQENR
                     STGGREFKFLSGPVFANIILADEVNRTPPKTQAALLESMQERQVTIGGVRHPLPSPFF
                     VLATQNPIEQEGTYPLPEAQQDRFMFKVFVKYPSYEEEFRIAETTTSELHAAVSDVLT
                     GDDILRLQQMVRRVPVAPFVTHYALRLVRATRVHEGDVPGFMRDWVSWGAGPRGVQYL
                     LLGGKARAMLAGRFFVTTDDIRSIAQPVLRHRVITNFNAESSGITPDKVIDRLLAEIP
                     ERRPGDEVAPELKRAFA"
     gene            6201..7238
                     /locus_tag="VFB80_21635"
     CDS             6201..7238
                     /locus_tag="VFB80_21635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN36452.1"
                     /translation="MEQREGKKDRDRAVWLLPLPIWLIAIAVHLFLLTLGAIFIKFGR
                     PGADGAKVERPVAALVAEPIARQVEYFAEEKPAPPQSPPAAEGSANAPNGSTLGAAIP
                     SGLPPVLPGVSLPQLPGGLPPADDLVGVAAPGGPAKRVSILPELGDAEILAADAAIPR
                     EVVPTGPTAQLSLFGSAAAEGRSFVFVIDRSQSMGGGGLGAIQAASSELKARLGQLTE
                     QQTFQVVAYNQQAVYFTEGGLIPATDDNKRRLVKFVEDIAAYGQTEHTRGLLAALRLR
                     PEVIFLLTDAGDPVMKPADLHVVREATRGRTSIHCLHFGRGKQESSENFLARLAAENR
                     GSYTYIDMNAR"
     gene            complement(7247..7705)
                     /locus_tag="VFB80_21640"
     CDS             complement(7247..7705)
                     /locus_tag="VFB80_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN36453.1"
                     /translation="MAQTDRQLITAWWSAAWTDGLWAAAWSKSIDGLTAEQAAWKPPG
                     VAGQRHSIWQLVLHLIFWRESWLRRLATGQKPTPDETARLNFPEIADRSEAAWAEARR
                     RLADTQQQIAAALASVNPDADLLMHFLPHDCYHFGQINYLRAMQGLPPIE"
     gene            complement(7718..8791)
                     /locus_tag="VFB80_21645"
     CDS             complement(7718..8791)
                     /locus_tag="VFB80_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN36454.1"
                     /translation="MKDESEASITKGLAWLVAQQAADGGWHSKAYGQLKDGASVTALA
                     VHAISHAREKDKLTLEESAAVTKGFDFLRRGLAKRGTIASPDGSLDFPTYAAALWLEA
                     TLRMARNDERAKVIDYLVQAQVAEPRGFESSSASYGGWDFLGPADAQGITTGTNVSVT
                     CFVLEALAAEQASGGRKPTEAMETALVRGKAYALRCQQPDGGFAFTCEPASLNNKAAF
                     LDEKLTQPRSYGSATCDGIRALVACGMKAADEPVAKALAWLVKRTGLEVVPGFETLPP
                     EAGWQRGLRYYYYASLARALAHFAASDREVRRAALAKHIVSLQKSDGSWQNESDRMRE
                     NDPLIATPLALLALAAAGAVDPS"
     gene            complement(8896..9984)
                     /locus_tag="VFB80_21650"
     CDS             complement(8896..9984)
                     /locus_tag="VFB80_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36455.1"
                     /translation="MTISRRSLLAAAPAAALPLVLPRILSAQEPPKPAESKPAETKPW
                     QEFTPQSDRAVQRGNEFLMKTLGRNGGCGVDIGQPDDIGCTAMAALALMAQGNTPVEG
                     PHSREVQRILTYMLQKTENMPADDITSQMGTQLQNKIGRHAHSFFAATFLAEVIGEGW
                     DTEPVRTGLRRVVTAIVGSQTAQGHWGDQSWAPMLGTVMGWVSLRASHFAGMKVGGSS
                     DLTAKYLVQQMQTQLAQHQGSWMHTLYKNATGIRVLYALGMDSEPIAKKALEDVKQLV
                     NKDNTAFSQAGGEEYLAFHLITETMLQKGGDDWATWFPSMRDKIIGVQNADGSWTGHH
                     CITSRTFCTAAAILVLTAPNRYLPISQA"
     gene            complement(10104..10346)
                     /locus_tag="VFB80_21655"
     CDS             complement(10104..10346)
                     /locus_tag="VFB80_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36456.1"
                     /translation="MERERLLLNPALIGVLLLGMLAAAYISGYTLRCRVVTVMRMPSG
                     PATVRTYPSWLEAQVYSPGGYVESLATGQVVYVGHR"
BASE COUNT         1687 a         3499 c         3529 g         1936 t
ORIGIN      
        1 agatcacgcc cccgtcgatg gccatcgact ggtgcacgcg gtcggcgaag agcagcacct
       61 ccgcgggcga cttgggcctg gtggacgacg cttgctgcgg catgtagccg taagccatcg
      121 ccatctgggc cagcatctgc gacatgctgt ccagctcgaa gcggttctcc cacgccgact
      181 gccacaccgg ctgctggctg tgggccacgg acgagtagca aacgaggcgg tcgggcgtct
      241 tgagatacac gcggccgccg tcgaacagca gccggccggc gggcttccag ccgccggccc
      301 gccaattggt gatcaggtct tcgcggctga cgggcgcttg ctgttgcacc tgcccgcctt
      361 gactcatggc catgccgccg cgcatcacga cgacgccgcc catgaacgcg ccgccgccga
      421 aatactgctg ctggcccggc agcgtcgcca aggccagcgg atcttcgcgg acccacaatt
      481 ccgagagcgt gcggctggtg accgcttcgg gcagcgcggg catgaaaccg gtgcggccgg
      541 gattgcccag cgccatgtgc cagtctttcg cggccggccc gacgccggag gtcgcggcag
      601 ccgagcggac atcggccacg atcagatcaa cgacgtcgct cgaaggacgc ggcccgccgg
      661 cggacgtgag tttggtcagc gcctgttcgg ccgattggcg gtcgcccatg cgagcgctgg
      721 ctaccgccag gcggagcagg agttcgctcc gcggaatcga cgggtcggga tggctgtcga
      781 gaatcttgtt gagcaggcgg ctcgcgccga caaagtcgta ccggtcgagg gccaggcagg
      841 cgagcttata cgccgcgtca tcgcccacgg agctgagaaa gtagcggcga acgacagcgg
      901 ccagggcgtc ttcctcgccg gacgggccgg ccgcggcgag cagcgcttgg gcctcggcgt
      961 cggccgaggt gcgataggtg gccagcgcca gcggcggcag cttcgccagc gtgcgctcca
     1021 cctgctgggc cagcgaggtg tagatggcga cgggcacggg cgacgactgc gagtgctgca
     1081 gcatcagcgt gtcgcccgcc tcgtcgagca ctttctgcca caggaccgcg gccaggtcga
     1141 gccgcccatc ggcgacgcac aggtcggccc gcttgagcag ccgctgctgc tcgggatcgg
     1201 tcttcagcgc cgctccgccg gggaagaggc tctcgctggg atccttggcc gaatcgtcgt
     1261 cggcgtcccg cggccgcgtc gtcgttggtg cggaaggact gggcggcggc ttgagctgtg
     1321 cctgacccgc gtcgccgccg aacagcagca gcgcgagcgg ggccaggacc atcagcgtcc
     1381 aatgtcggcc attgggcgac gtgcggcgca tcggcaagcc tccgtgattt tcctgaaacc
     1441 cagacattca gtctatgttc ggctgcggcc gaaaaaccac cccagcagct gctcgccggc
     1501 cagcactgcg accacgcccc acagcaaata ccaccagatt tccgtttgcg tgctggcgtc
     1561 ggccagcgac tgggccgcct tcgccgagac gatctcgacg ttcgtgccgg ccagctcctt
     1621 ctgcatcgtt tcgcgatccg cccgcttcag gtccccttcg ggcggatcga tattggcggc
     1681 gaacagtacg ctgtcggacg ctccgtcgcg gcgggccagc ttcagctcat agaaaccctg
     1741 cgacgccgcg gccggaaact ccatccgcca gatcgtctgc tggtctgcgc cgggccctgc
     1801 gggcggcgcg gcctggacgt tggccttcag ctccttcggg ccgaccagcg gcgcgtcgag
     1861 ctcgtactgc gtcaggtcga tccactgctc cagcggctgc ccgacggtta ccaggccttt
     1921 gctgccgcga tcggccgcca ggtaccggac aaaatcctgc acgacgagca gatagctggg
     1981 gtcgctggtc cagttgtgcc agtcggcgtc ggcggggatc gtgaaggcga cgacgcggcc
     2041 cttgccgagc ggcttctcga cgatcgccgg cgagtcctcg aggtcgttga gcttggcgac
     2101 gatcgagacc tctttgccga gctgctcctt cttcacgctc gcgccccacc agcgaaagac
     2161 cttcacgctt tcgaggagcg ggttgttctg gccggcgaac tgcttgaggg cttcgtggct
     2221 ggcgtcttcc accttgaggc tggcccacgt tttctcgctc tcgtcgccgc gaatgttttc
     2281 gagcttgacc ggcgacaggc cggcgccgga ccgccagtac tgatcgttga agaactgctc
     2341 gtcgatctgg tcgccgggca tgagcaccag gcccccgccg ccttcgaccc acttttccag
     2401 ccgctggatg ttctcggcgg tcttgtcgcc gaggcgatag ttgttgagca ggaaaatgac
     2461 ctggaactta tcgagggcca gcgattccag ctcgttctcg gtgacgatcg tcgccgccac
     2521 gcccgacggc accgggccga tgggcgccag ggcccgccgg agatagaacg actcggcctt
     2581 gccgaagtcg gcgcttggat cgccgtcgat gatcagcgtc ggaatgccgc ggaccaggcg
     2641 ggctggaaaa taggccacgc tgtcggccgg cagccggtcg tcggcgcctt gctgggccgt
     2701 ggtcaattcg accttcactt gccgcggcgg cagcggccgc tcggcgctct cggcctcctc
     2761 gtcgcccgag aaggtgaagt tgaagcggac cgaggtcgtc tggcccgggg cgagctggtc
     2821 gatcgtcgtt tccaccggca gcgagtcgcc ggcgctgagc ttgacgcgaa tatcgctggc
     2881 ggcagtcgtg ccgttgttgg tcacggcaac gtcgaaccgc gactgcacgc cttggaccag
     2941 cgtgccttcg gggcggatct cgctgacggt gagattgcgg tcttcgtctt cgcccgcatc
     3001 gatcaggaag cagccctgca cgagctttgc gacacggccc aggatcttga gcggcgcttc
     3061 ggggttggcg tcgccttctt tccagtcccg ccgccgcagg tcgctgagca cgtagaccac
     3121 gcgattgatg ttcgggggct gcgacgccag gtactgctcg agctcggcca gcgaggcgtc
     3181 gagccgcgcg gtgccgtcgc cgggttcgag cttctcgatc gacgcctgaa tgtcgctgac
     3241 gttgtccgtg ccgaggtgcg tggcgctaaa cagcgggcgg tcgggctgcg aggtgaggag
     3301 cagcgtaaag gaattgtcgg cctgatcctg ggccaaagaa cccgcgagat ccgacagccg
     3361 cttgcgggcg acttcgaacg cggcctcgtt gcccaggcgg gcctgcatgc tgagcgagtc
     3421 gtcgagcagg acgatccgct cgaactgggc ggcgttgagc agacccgcgg tgacgctggt
     3481 cggaatgaac ggccgggcca gcagcatgcc gatcaggaag accgccaggc accgcaggag
     3541 cagcaggatc agattttcga ggcggatgcg gcggcggttc ttcttgtcgg cgtcgagcag
     3601 gaactccatc gccgcccaat cgacaatctt gaacttccgc ttgttcaaga ggtggatcag
     3661 gatcggaatc aagatcgccg ccgctccggc tacgaacagc cagggattga ccagccagct
     3721 ggcgagcgat tcgatctgag caatgagggg catattcgta gaacaagcgt cctcgcttgt
     3781 tcatcgtcat ttcggttgga ggcatgagca agcgaggacg ctcgctctac ggttgtcagt
     3841 ggtgtctggc cgtccggcga atcttttgcc ggaaggtgag gtagctgccg aggaccgcgt
     3901 cgagcggctc cttcatgttc atcagcacgt aatcgacgcc ggcgctggcg caggtcttgc
     3961 gcacgtctcc caggaaccgc tcgaccgcgt ccaggtagcc gcgccgcagg gcccgcggct
     4021 cggtgtgcag ctggatcggc tcctccagtc cgcggaagag cgtgttgtcc tggaacggga
     4081 acgtcagctc gtcgtcgtgc atcacctgga agacgatgac ctcgtgccgc cgcaggcgaa
     4141 attgcttcag cgattcggcc agcgtcgaga tcggcaggaa caggtccgac aagagcacga
     4201 tcatcccgcg gcggcggatt tgccgggcca attcgggaaa gacctgggcc acgtccgtct
     4261 tgtcgtccgg cgttgtctgc tccagctcat gaaacagcag cttcaaatgg ttcggatgcg
     4321 agctggccgg caagtttttc tggactttcg tcgtgaatgt gaccagcccg acggcgtcct
     4381 gctgctgctg caagagatac gccaggctcg ccgccgccgt cgccgcatag tcgtacttcc
     4441 gccagccttc gccgtaccgc atcgacttgc tggcgtcgag caggatggtg cacttgaggt
     4501 tggtctcttc ctcgtattcc ttgatgtaga gccgatccga ccgcgaccag accttccagt
     4561 cgatgtgccg cgtgtcgtcg ccgggggaat actcgcggtg cgtggcgaac tcgacggcga
     4621 agccgtggta agggctgcgg tgctggcccg tgatgaaacc ctcgaccacc agccgcgcgc
     4681 ggacatccag ccgcttgatg cggtccagcg tttttgggtc gaggtattgc ggtaaagcca
     4741 tagggttcag ggctcggggt tcagggttca ggtcggcatc gttgtctcat gctgaaccct
     4801 gaaccctaac cactctcatg cgaaggcccg tttcaattca ggcgcgactt cgtcgccggg
     4861 acgccgttcg gggatttcgg cgagcaggcg atcgatgact ttgtcgggcg tgatgccgct
     4921 cgattcggcg ttgaagttcg tgatcacgcg atgccgcagg acgggctgcg cgatcgaccg
     4981 gatgtcgtcg gtggtgacga agaaccggcc ggcgagcatc gctcgcgcct tgccgcccag
     5041 cagcaggtac tgcacgccgc gggggcccgc accccagctc acccagtcgc gcatgaagcc
     5101 cggcacatcc ccttcgtgga cgcgcgtcgc ccgcaccagc cgcagggcgt agtgcgtgac
     5161 gaacggcgcg accggcacgc ggcggaccat ttgctgcagc cgcaggatgt catcgccggt
     5221 gagcacgtcg ctgacggctg cgtgcaattc ggaggtcgtc gtctcggcga tgcggaactc
     5281 ctcctcgtag ctggggtatt ttacgaagac cttaaacatg aagcggtctt gctgggcttc
     5341 gggcagcggg taggtgcctt cctgctcgat cgggttctgc gtcgccagca cgaagaacgg
     5401 cgaaggcagc gggtgccgca ctccgccaat cgtcacctgc cgctcttgca ttgactccag
     5461 cagagccgct tgcgtcttgg gcggcgtgcg gttcacttcg tccgccagga tgatgttggc
     5521 gaacaccggc ccgctcagga acttgaattc gcggccgccc gtcgaacggt tctcttgaat
     5581 gacttccgtg ccggtgatat ccgatggcat caggtcgggc gtgaactgaa tgcggctgaa
     5641 ctggagcttg agcgagcggg ccagcgtgct gaccatcagc gtcttggcca ggcccggcac
     5701 gccttcgagc aggcagtgcc cctgggcgaa gatcgcgatc aggagctggt cgatcacctc
     5761 gtcctgcccg acgatgacct tcgccagctc cttgcggacc gacgcgtacg cgctcttgag
     5821 gttctccaag gcctggaggt cgtcggaact cggttgaacg gtgccaatca ttggtaattc
     5881 ctgacgcaat gtgcctgatg ctgatgaact agacgaaagg gaatgcaggc tggttctgcc
     5941 ggccggcgcg caaagcctgc ctgccgtttg tcgcctggca gcccgggaaa gttcagctct
     6001 ggtggaggag cggaagcggt cgcaggtggg cgcagtagca agcctcctcc gtttcccctg
     6061 gaagtcgccg tacattctaa atcaactgcc ggccattgcc agcagttgta acgaagccgc
     6121 agcttgcctg cggcccaggc gggaatcgat aatggtcgcc tggggcggag cgagggaaac
     6181 acgaggcagc gcgagggaac atggaacagc gcgaggggaa aaaggaccgc gaccgggccg
     6241 tttggctgct gccgctgccg atctggttga tcgcgatcgc ggtgcacctg tttctgctga
     6301 ccctgggggc cattttcatc aagttcgggc ggcccggggc ggatggcgcg aaagtcgagc
     6361 gtccagtagc agccctggtc gctgagccga ttgccaggca agtggagtac ttcgcggagg
     6421 agaagcccgc tccgccccag tcgcctcctg ccgctgaggg atcggccaat gctcccaatg
     6481 gatcgacgct cggcgccgcg attccttcgg gcctgccgcc ggtgctcccg ggcgtctcgc
     6541 tgccgcaatt gccgggcgga ttgccgccag ccgacgacct ggtcggcgtg gccgcgccgg
     6601 gcgggccggc caagcgagtc agcatcctcc ccgaactggg cgacgccgaa atcctcgccg
     6661 ccgatgcggc gattccgcgc gaagtcgtgc ccaccgggcc gacggcgcag ctgtcgctgt
     6721 tcggctcggc ggctgccgag ggccgctcgt tcgtgttcgt catcgatcgc tcgcaaagca
     6781 tgggcggcgg cggtttgggg gcaattcagg cggcatcctc cgaactgaag gcccgtctcg
     6841 gtcagctcac cgagcagcag acctttcagg tcgtggccta caaccaacag gccgtctatt
     6901 tcacggaggg cggcctgatc ccggcgaccg acgataacaa gcggcggctg gtcaagttcg
     6961 tcgaagatat cgccgcgtac ggccaaaccg agcacacccg cggcctgctg gctgctttgc
     7021 ggttgcggcc cgaagtcatc ttcctgctca ccgacgccgg cgacccagtc atgaagcccg
     7081 ccgacctgca cgtcgtccgc gaggccacgc gcggccgcac gagcattcac tgtctgcact
     7141 tcggccgcgg caagcaggaa tcgtccgaga acttcctggc tcggctcgcc gccgaaaacc
     7201 gcggcagcta cacctacatc gacatgaacg cccgctgagc cgctggtcac tcgatgggcg
     7261 gcagaccctg catcgcgcgc aggtagttga tctggccgaa gtggtagcag tcgtgcggca
     7321 gaaagtgcat gagcagatct gcgtccggat tgacgctggc cagcgccgct gcgatctgct
     7381 gctgcgtgtc ggccagccgg cggcgggcct cggcccaggc ggcttccgat cggtcggcga
     7441 tttcgggaaa attcagccgg gcggtttcat cgggagtcgg cttctggccg gtcgccagcc
     7501 ggcggagcca gctctcgcgc cagaagatca ggtgcaagac cagttgccag atcgagtgcc
     7561 gctggccggc gacgccgggc ggcttccagg cggcctgctc ggcagtcaga ccgtcaatcg
     7621 acttcgacca cgcggcggcc cacaggccgt cggtccaggc agcggaccac caggcggtga
     7681 tgagttgtcg atcggtctgg gccacggaaa atccagctta gctcgggtca acggcaccgg
     7741 cggcagccag cgcgagaagc gccagcggcg tggcgatcag cggatcgttc tcgcgcatgc
     7801 ggtccgattc gttctgccag gagccgtcgc tcttctggag cgagacgatg tgtttcgcca
     7861 gcgccgcgcg gcggacttcg cggtcggagg cggcgaagtg cgcgagggcg cgggccagcg
     7921 acgcgtagta atagtagcgc agcccgcgct gccagccggc ctcgggcggc agcgtctcga
     7981 aacccggcac gacttccagc ccggtgcgct tcaccagcca ggccagcgcc ttggcgaccg
     8041 gttcgtccgc agctttcatt ccgcaggcga cgagcgcccg gatgccgtcg cacgtcgccg
     8101 acccatagga tcgcggctgc gtgagtttct catcgaggaa cgccgccttg ttgttcagcg
     8161 acgccggctc gcaggtgaac gcgaagccgc catccggctg ctggcagcgc agggcgtagg
     8221 cctttccacg gaccagcgcg gtttccattg cctcagtggg cttacgccca ccgctcgcct
     8281 gctcggcggc cagcgcctcg agcacaaagc aagttaccga aacgttcgtc cccgtcgtaa
     8341 tgccctgcgc gtcggccggg ccgaggaagt cccagccgcc gtagctggca gagctgcttt
     8401 cgaacccgcg cggctcggcc acctgtgctt gcacgagata gtcgatcact ttcgcccgct
     8461 cgtcgttgcg ggccattcgc agcgtcgcct ccagccacag cgccgccgca tacgttggaa
     8521 agtcaagcga gccgtcgggg ctggcgatcg tcccacgctt ggccaggccg cggcggagga
     8581 aatcgaatcc cttcgttacg gcagcgctct cttccagggt cagcttgtcc ttctcacggg
     8641 cgtggctgat ggcatgcacc gccagcgcgg tcaccgaagc gccgtccttc agctggccgt
     8701 aggccttcga atgccagccg ccgtcggctg cctgctgggc gacgagccag gcgagccctt
     8761 tcgtgatgga tgcctctgac tcgtccttca tccgcgtcat ccgcgttcat ccgcggctca
     8821 atagctgctg gcttggttgt ggcacgctgc gcggcgcaca gccacccaga ccgcgaccac
     8881 tggtcgcggc tttgatcagg cctgcgagat cggcaaatac cggttcggtg cggtcagcac
     8941 caggatcgcc gcggcggtgc agaacgtgcg gctggtgatg cagtggtggc cggtccagct
     9001 gccgtcggcg ttctgcacgc cgatgatctt gtcgcgcatc gaggggaacc aggtggccca
     9061 gtcgtcgccg cccttctgca gcatcgtctc ggtgatcagg tggaacgcca ggtattcctc
     9121 gccgccggcc tgcgaaaacg cggtgttgtc cttgttgacg agttgcttca cgtcttccag
     9181 ggctttcttg gcgatcggct cgctgtccat gcccagggca tagagcacgc ggatgcccgt
     9241 cgcgttcttg tagagcgtgt gcatccagct cccctggtgc tgggccagct gcgtctgcat
     9301 ctgctgcacc aggtattttg ccgtcaggtc ggacgagccg ccgaccttca tcccggcaaa
     9361 gtgcgaggcc cgcagcgaga cccagcccat caccgtgccc agcatcggcg cccacgactg
     9421 gtcgccccag tgcccctggg ccgtctgcga gccgacgatc gccgtcacca cccgccgcag
     9481 gcccgtccgc accggctcgg tgtcccagcc ttcgccgatc acttcggcca gaaacgtcgc
     9541 cgcgaaaaag ctgtgggcgt gccggccgat cttgttctgc agctgcgtcc ccatctgcga
     9601 cgtgatgtcg tccgccggca tgttctccgt cttctgcagc atgtaggtca ggatccgctg
     9661 cacctcgcgg ctgtgcggac cttcgaccgg cgtgttccct tgggccatca gcgccagggc
     9721 ggccatcgcc gtgcagccga tgtcgtcggg ctggccaata tcgaccccgc agccgccgtt
     9781 gcggcccagc gtcttcatca ggaactcgtt cccccgctgc acggcccggt cgctctgcgg
     9841 cgtgaactcc tgccagggct tcgtctcggc aggcttcgat tcggccggct tcggcggctc
     9901 ctgggccgaa agaatgcgcg gcagcaccag cggcagcgca gccgccggag cggcggccaa
     9961 gagcgagcgg cgggaaatgg tcatcgcagt gaactccatt cgtcgcggct acgacgattt
    10021 gtctattgtt ggcgagcccg cagggcggcg tcaatcaagt tgttgcgcct cgtagccgac
    10081 gcagcctgcg tcggcgcgcc gcgtcaacga tgccccacat acaccacttg gcccgttgcc
    10141 agcgactcga cgtatcctcc cggcgaatat acctgggcct ccagccacga cggatacgtg
    10201 cggaccgtcg ccggcccgct gggcatacgc atcaccgtca cgactcggca gcggagcgtg
    10261 tagccgctga tgtaagccgc cgccaacatg cccaacagca gcacaccgat cagcgccggg
    10321 ttcagcagca gtcgctctct ttccatgcgg ctcatcgtat ttcggattgg aacagaaggc
    10381 agcaaaggaa gcaaagtcgg ccgcgacttc cgcgaccggt tatgctgcat aattccatct
    10441 tttcctggag tcgccattcg gtcataagcc tcgctgcgga cattacttag ggcgagagct
    10501 gccccgcatg catatgcaag ttatgcagtg aagcgtcttc gtgctgacgc ctggtgcatt
    10561 tccggcctcc cttggtttcc ttcgttgact tctgttcgtc ctcgccgcgt gatctgtctg
    10621 cgtctgcctt ctttgctgcc attgctgtct t
//
LOCUS       DASRJB010000824         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_531241, whole genome shotgun sequence.
ACCESSION   DASRJB010000824 DASRJB010000000
VERSION     DASRJB010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531241"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..854)
                     /locus_tag="VFB80_21660"
     CDS             complement(6..854)
                     /locus_tag="VFB80_21660"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZN36457.1"
                     /translation="MRSVFLLTAAFVFSISSIHAAEPTPAQKLIAAPRVLISAHRGNS
                     SVAPENTLPAFQSALDAQADLVELDYFHSADGVPVVIHDEILDRTTDAEAVLGQPKLL
                     IGDLRLADIQKLDVGSWFSDKFAKTRLPTLAESLDLIQTKSVTLIERKAGDAKTLVKL
                     LEDKKLLDQVVVQAFDWHFVAECRHLAPRLALGTLSSKPPSIEQLQGAAATGADLIVW
                     NHEKIGPTQIKQIHALGKKAWVYTVDDPDRAAALIAAGIDGIITNKPAEMRKLRASAP
                     SPSPQP"
     gene            complement(899..1459)
                     /gene="frr"
                     /locus_tag="VFB80_21665"
     CDS             complement(899..1459)
                     /gene="frr"
                     /locus_tag="VFB80_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZN36458.1"
                     /translation="MAADDILLDTEDRMDKAIQHLKTSLAGIRTGRANPGLVDSLKVE
                     VYGSQTPMKSLASVGAPEPQQILVRPYDPGTIKEIERAIINSGLGFAPQNDGRVIRIN
                     VPPLTTDTRKKLVARIKELTEEAKVSVRNVRRDGNKAAEQGEKDGDLTEDERDKCKDD
                     IQELTKKYEGLAADMAKKREDEVMEG"
     gene            1683..2366
                     /gene="rsmG"
                     /locus_tag="VFB80_21670"
     CDS             1683..2366
                     /gene="rsmG"
                     /locus_tag="VFB80_21670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZN36459.1"
                     /translation="MNSDSSQPATTAASDTLSAALVRHGLALEPPQIELLDRYARLLW
                     DWNTKINLTRHTDYDKFAGRDIADTLQLAAHLKPGEEVLDVGTGGGVPGLPLAILRPD
                     LKVTLCDSVAKKVRVVAEISEQLGLPIEAHAVRAEELVEDGRYDALVIRAVGPLWKLL
                     ATFKSHWRSFRRMLVVKGPKWPEELNEAKRRGLVNQLQIKTAAEYAMPGADGKSVILK
                     IWPKNVRER"
BASE COUNT          388 a          746 c          803 g          455 t
ORIGIN      
        1 gagggctagg gctgggggct agggctgggg gcggaggctc gcagcttccg catctcggcc
       61 ggcttgttcg tgatgatgcc gtcgatgccg gcggcgatga gggcggcggc gcggtctggg
      121 tcatcgacgg tgtagaccca ggcctttttg ccgagggcgt ggatctgctt gatttgcgtg
      181 gggccgatct tttcgtggtt ccagacgatg aggtcggcgc cggtggcggc agcgccctgc
      241 agctgctcga tgctcggcgg cttgctgctc agcgtgccca gtgccaggcg cggcgcgagg
      301 tgccggcact cggcgacgaa gtgccagtcg aaggcctgca cgacgacctg atcgagcagc
      361 tttttgtctt cgagcagctt gacgagcgtc ttggcgtcgc ccgccttgcg ttcgatcagc
      421 gtaaccgact ttgtctggat caggtcgagc gactcggcca gcgtgggaag cctggttttg
      481 gcgaacttgt cgctgaacca ggagccgacg tcgagcttct ggatgtccgc cagccggagg
      541 tcgccgatga ggagcttggg ctggccgagg acggcctcgg cgtcggtcgt gcggtccagg
      601 atttcgtcgt ggatcacgac gggcacgccg tcggccgaat ggaagtagtc gagctcgacc
      661 aggtcggcct gggcgtcgag cgccgactga aacgccggca gcgtgttttc gggcgccacg
      721 ctcgaattgc cgcggtgggc cgagatcagc acccgcggcg cagcgatcag cttctgagcc
      781 ggcgtcggct cagcggcgtg aatggaggaa atggaaaaca cgaaggccgc cgtgagcaag
      841 aaaaccgatc gcatctgtcg cctcctgacc cctggctcct gactcccgac tcctggccct
      901 acccttccat gacttcgtct tcccgcttct tggccatgtc ggcggcgagg ccttcatact
      961 tcttcgtcaa ttcctggatg tcgtctttgc acttatcccg ctcgtcctcg gtcaggtcgc
     1021 cgtctttctc gccctgctcg gcggccttgt tgccgtcgcg gcggacgttg cggacgctga
     1081 ccttggcctc ctcggtcagc tccttaatcc gggccacgag cttcttgcgg gtgtcggtcg
     1141 tcagcggggg cacgttgatg cggatcacgc ggccgtcgtt ctgcggggcg aagcccaggc
     1201 cgctgttgat gatcgcccgc tcgatctcct tgatggtgcc ggggtcgtac gggcgtacca
     1261 gaatctgctg cggctccggc gcgccgacgc tggccaggct cttcatcggc gtctgcgagc
     1321 cgtacacctc gaccttcagc gaatcgacga ggcccggatt ggcccgcccg gtgcggatgc
     1381 cggccaggct ggtcttgagg tgctggatcg ccttgtccat gcgatcctcg gtgtcgagga
     1441 gaatgtcgtc ggcggccatg ggcgtgctcc cagtcgtcat ttcccgtgaa ttcgctcatc
     1501 gtaccgctgc gccgcaaacc gcggcaagcg gagtcggcgg ccggcaggaa ttgcaaattg
     1561 ataagtgcaa aaggcaaatt gcaaattgaa aggtgtgaag gctcctacgg cgtggcgtca
     1621 atttgcaatt tgcaattctc attttgcaat ttgcaatcgg cccaccagag tacaatcgcc
     1681 gcatgaattc ggattcctcc cagccggcga ccaccgccgc gtccgacacg ctctccgcgg
     1741 ctcttgtccg ccacggcctc gcgctcgagc cgccgcagat cgaactgctc gaccgctacg
     1801 cccggctgct gtgggactgg aacacgaaga tcaacctcac gcggcacacg gactacgaca
     1861 agttcgccgg ccgcgacatc gccgacacgc tgcagctggc ggcgcatttg aagccgggcg
     1921 aagaagtgct cgacgtgggc acgggcgggg gcgtgcctgg acttccgctg gcgatcttgc
     1981 ggccggactt gaaggtcacg ctgtgcgaca gcgtggccaa aaaggtccgc gtcgtggccg
     2041 agatctccga gcagctcggc ctgccgatcg aagcccacgc ggtccgggcc gaagagctgg
     2101 tcgaggacgg ccgctacgac gccctggtga tccgcgccgt cggtccgctc tggaagctgc
     2161 tggcgacgtt caaatcgcac tggcgttcgt tccggcggat gctcgtcgtc aaaggcccca
     2221 agtggcccga ggagctgaac gaagccaagc gccgcggcct ggtgaaccaa ttgcaaatca
     2281 agaccgccgc cgagtatgcc atgcccggcg cggacggcaa aagcgtgatc ctcaaaatct
     2341 ggcccaaaaa cgtgcgggag cgatgaataa cccggacagg aggcagacag ga
//
LOCUS       DASRJB010000825         9243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1094144, whole genome shotgun sequence.
ACCESSION   DASRJB010000825 DASRJB010000000
VERSION     DASRJB010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094144"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFB80_21675"
     CDS             complement(<1..1483)
                     /locus_tag="VFB80_21675"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN36460.1"
                     /translation="MNLPLAFSRLFLLLVVILAQAGKITAAGLPRATPEEVGMDSGQL
                     ARIDALVAQGLAEKRMPGCVVCVGRHGKIVLLKAYGNKQLKPSEVPMTTDTVFDMASI
                     TKPTATATSIMLLVERGQLKLGQRVADLIPEFAANEKGEITIFDLLTHQSGLLPDNAL
                     ADYQDGREEALKKICALKLQAPLGTRFIYSDVNFILLGEIVRLASGKSVHEFSRENVF
                     QPLGMKETGYLPADELRKRAAPTEERDGRWMQGEVHDPRAFKLDGVAGHAGLFSTAED
                     LAIYAQMMLGRGEYGGVRILSPLTVAAMTRGYRLAAGSRSTMEGVPANPPAYFRGLGW
                     DKRTGYSINRGELLSDAAFGHGGFTGTVLWIDPELDLFFIFLSNRVHPDGKGLVNPLA
                     GRIATVAAAAIRDVEKRPAAGTVLTGIDVLKRDGFKQLAGRRLGLITNHTGKSREGET
                     TVKLLHEAQNVELTALFSPEHGFEGKLDVSKIGDTRDAATGLKV"
     gene            complement(1480..2979)
                     /locus_tag="VFB80_21680"
     CDS             complement(1480..2979)
                     /locus_tag="VFB80_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN36461.1"
                     /translation="MANLGWLDILVLVAYFATAVAVGLLVSGRSKSVEAFLLGDRNLP
                     WWAILGSIVATETSGVTVLSVPGHGYGQVAFKFLQIALGYVIGRAIIVYVLFPLYFRG
                     QLLTAYEVLNQRFGGLTRQAASLLFLIARNLGDGLRLYLVAIVLQKLAGIPLWLSVVV
                     MGVISTVYTFVGGMRSVIWNDCIQVGVYVLGGIVAVFVIAGQISGGFSEIARFAAETD
                     KWQVLDVRWSLTEPYTFWASLIGGAVLTIGTHGTDHSMVQRYLSARSQRDAGWALLVS
                     GLVVFVQFGLFLFIGIELACFFGQHPLPAGVKLKPDEVFAHFIVNHYPRNTGLIGLML
                     AAILASNLSSSLSASAAAVVNDFYLPWHKTPPESGYVLALTRGLTAVFGLLQIGLGIL
                     AQRVSTSGNTVVDSALAIASFVFGLLLGVFALGALTRRASQAGALAGAGFALALLLFL
                     QFGLPALTATDKLPTGIKIAFPWLSVIGSGTTFMVGYVTSLIAPQGTKA"
     gene            3069..3968
                     /gene="murQ"
                     /locus_tag="VFB80_21685"
     CDS             3069..3968
                     /gene="murQ"
                     /locus_tag="VFB80_21685"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZN36462.1"
                     /translation="MSRLTTEARNPASERIDTLSALEIARLMNAEDARVAEAVGREAE
                     PIARAIEIIAERLRAGGRLIYIGAGTSGRLGVLDATECPPTFSTPPEMVVGLIAGGYT
                     ALTRAVEGAEDHPEYAVSDLRQIQFSRGDVLVGIATSGRTPYVIGGLKYAREVEAAAI
                     GLACNDGSALAEFADLMITPVVGPEVISGSTRLKAGTATKLVLNMLTTGAMVLLGKTY
                     GNLMVDLKATNTKLVARTRRMVAMLTGVSEETAESLLGSCGGELKTAVVAHRHGIPAE
                     AAREMLRRAGGQLRIALESPTHG"
     gene            3961..4905
                     /locus_tag="VFB80_21690"
     CDS             3961..4905
                     /locus_tag="VFB80_21690"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZN36463.1"
                     /translation="MAEHQEFVIGVDGGGTKTAAWLASLVDDTILGRGQAGPGNPRAA
                     GFEVAQANIAAAVAAAFDDAKLSRQAVAAACFGLAGAGREVEQQLIADWALGRRIAKK
                     VRVCGDAELILAAASPENCGVALIAGTGSLAWGRNQAGETTRCGGWGYLLGDEGSGYA
                     IALAGLRAAMRAADGRGPTTGLLPAFLRELGAATAPELIGKVYSPEMTRERLAGLASV
                     VFEYRSTDEVAQRIVADAAGELAAMVTTVATELKLPPQGYMLAISGGVLLNQLNYLEQ
                     FLFTLWAHTHSGESQPARCVKVEEPVRGAVALARLLAR"
     gene            complement(4981..6087)
                     /locus_tag="VFB80_21695"
     CDS             complement(4981..6087)
                     /locus_tag="VFB80_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZN36464.1"
                     /translation="MRSHFIRLSIGLVLALLSLQPASAEALPDGRYLYVVCPGIRNYL
                     EFGGAGILVFDIDHGHKFVKRIGTPASREEKPDNIKGVCACAATKRLYFTTTKKLYCV
                     DLATEQTLWETTPPNGTDRMSITPDGKTIYVPSFEKDTWNVIDAADGKLITSIETKSG
                     AHNTVVSRDGSRMYLGGLKSPLLFVADTKTHDIVQKAGPFAGSIRPFTVNAAKTRAYI
                     CVNDCLGFEIGDLTSGQKLHRVEVAGVKPGMVKRHGCPSHGIGLTPDEKEVWVVDAAN
                     QQVHVFDNSVEPPKQKESISLREQPGWITFSLDGKLAYPSTGEVIDTATKKIVTALAD
                     EKGGQVHSEKMVEIQWRGGQPVASGDQFGVGRAK"
     gene            complement(6124..6549)
                     /locus_tag="VFB80_21700"
     CDS             complement(6124..6549)
                     /locus_tag="VFB80_21700"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZN36465.1"
                     /translation="MPFSLARTTIFACGFALLGAALATLALNVTSPALAQDKEAAPLR
                     HIVLFKFKADATKEQVQEIVAGFGQLPKKIDGITAFEWGTDVSPEKRSEGFTHCFVVT
                     FKDAKARDTYLPHDAHQAFVRLALPKVEKVLVVDYFAQK"
     gene            6636..7265
                     /locus_tag="VFB80_21705"
     CDS             6636..7265
                     /locus_tag="VFB80_21705"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZN36466.1"
                     /translation="MYIPAHFAAPDQESLYAAVERYSFATLVSLRPSGGITASHLPLL
                     LERPRTDGIQSLPAALVGHMARANPQWRDAAGQEVLAIFSGPHAYISPTWYEAKQVVP
                     TWNYVAVHACGQLELVEDATAAEAILRRTVALYEAGQPRPWTLDEPAEFVERLTAQIV
                     AFRIPIARIEGKWKLNQNHPADRRAKVIAALEQQGDENSREIARLMQSS"
     gene            complement(7312..8172)
                     /locus_tag="VFB80_21710"
     CDS             complement(7312..8172)
                     /locus_tag="VFB80_21710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN36467.1"
                     /translation="MRNPARILLATRDEGRQRAWTEALRTARCQVVTTNGAPAEDIDV
                     LVIDQPLTDANIAIDEERLSRGQMGMVAVGVGLPADVSLPEDHSSRELRLACLLLAEI
                     VRLRRQREASRRQEKVLTHLALSDPLTGLPNRRAWEQQLNDRLGGAAEGSAWCMALLD
                     VDLFHEINDRQGHLEGDSCLRRIADRLAGGVRRAGFLARLGGDEFAVLLEGIAADRAA
                     AVVDLIRIQAGEDGDAAANRSPLKLSAGWAVLTGPANKAAAQDALERADAALREAKQS
                     GRNRTRPANS"
     gene            8360..>9243
                     /locus_tag="VFB80_21715"
     CDS             8360..>9243
                     /locus_tag="VFB80_21715"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZN36468.1"
                     /translation="MNASTADFSTIPAVWTRQHLLDLESLSAEELTCLLDAATAFKDA
                     TDGCRRKISVLSGRTLANLFFENSTRTRNSFSLAARRLGADTVEFSSSGSSVSKGETF
                     IDTAKNIEAMNVDAVVIRHQTPGTPHLLAKNLNCSVINAGDGPHEHPTQGLLDILTIR
                     ERRGTLAGLTVALVGDIAHSRTARSNIWGLKKLGAHVIVCGPATLVSRRWEEFGVEVA
                     YNLDEILPRCDVLNLLRIQFERQHTRPFPSVREYALLYAMNRERLNRAKDDILIMAPG
                     PINRGVEITPEVADGPHSV"
BASE COUNT         1641 a         2988 c         3042 g         1572 t
ORIGIN      
        1 agactttcaa gccggtggcg gcgtcgcgcg tgtcgccgat tttgctgacg tcgagcttgc
       61 cctcgaagcc gtgttccggg ctgaacagcg ccgttagctc gacattctgc gcctcgtgga
      121 gcagcttgac cgtggtttcg ccttcgcggc tcttgccggt gtgattggta atcaggccga
      181 gccttcggcc agccagctgc ttgaagccat cgcgcttcag cacatcgatg ccggtcagga
      241 ccgttccggc ggctgggcgt ttttccacgt cgcgaattgc cgcggcggcg accgtggcga
      301 tgcgaccggc gagcggattc accagcccct tgccgtcggg atgcacgcgg ttgctgagga
      361 agatgaagaa caggtccaac tccgggtcga tccagagcac cgtgccggtg aagccgccgt
      421 ggccgaaggc cgcgtcggaa agcaactctc cgcgattgat cgagtaaccg gtgcgcttgt
      481 cccaccccag gccgcggaaa taggccggcg gattggcggg aacgccctcc atcgtgctcc
      541 gcgagccggc cgccaggcgg taaccgcggg tcatggcagc gacggtaagc ggcgacagaa
      601 ttcgcacgcc gccgtattcg ccgcggccga gcatcatctg ggcgtagatc gccaggtcct
      661 ccgccgtcga gaacagcccg gcatgtccgg cgacgccatc cagcttgaac gcccgcgggt
      721 cgtgcacttc gccctgcatc caacggccgt cgcgctcctc cgtcggcgcg gcccgcttgc
      781 gcagctcatc cgccggcaaa tagccggtct ctttcatccc cagcggctga aagacgttct
      841 cccgcgagaa ttcgtggacc gacttgccgc tggccaggcg gacgatttcg cccagcaaga
      901 tgaaattcac gtcggagtag atgaaccgcg tgcccagcgg cgcttgcagc ttgagtgcgc
      961 agatcttctt cagcgcttcc tcgcggccgt cctgatagtc ggccagggcg ttgtcgggca
     1021 aaaggccgct ctggtgggtc aacagatcga aaatcgtgat ctcgcctttt tcgttcgccg
     1081 cgaattccgg gatcaggtcg gcgacgcgct ggccgagctt cagctggccg cgttcgacga
     1141 gcagcatgat gctcgtggcc gtcgccgtgg gcttggtgat tgaggccatg tcgaagaccg
     1201 tgtccgtcgt cattggcact tcgctcggct tcagttgctt gttgccgtag gccttgagca
     1261 gcacgatctt gccgtggcgg ccaacgcaga ccacgcagcc gggcatccgc ttctcggcca
     1321 gcccctgagc cacgagcgca tcgatccggg cgagctgccc gctgtccatg ccgacctctt
     1381 ccggcgtggc gcggggcagc ccggcagccg tgatttttcc ggcctgcgcc aggatcacca
     1441 ctagaagcag aaagaggcgc gaaaaggcaa gcggcaagtt cacgctttgg ttccttgagg
     1501 ggcgatgagc gaggttacat agccgaccat gaacgttgtg ccagagccga tgacggagag
     1561 ccagggaaat gcgatcttaa tgccggtcgg gagcttgtcg gtcgccgtga gcgccggcaa
     1621 gccgaactgc aagaataaca gcaaagcaag agcgaatccc gcaccggcca gcgcgccggc
     1681 ctgggaggcg cggcgggtga gcgcgcccaa ggcgaagact cccagaagca acccgaaaac
     1741 gaagctggcg atcgccaggg cgctgtccac gacggtgttg cccgaagtcg aaacccgctg
     1801 cgccaaaatg ccgagcccga tctgcagcag gccgaacacc gcggtcagcc cgcgggtcag
     1861 cgccagcaca tagccagact ccggcggggt cttgtgccag ggaaggtaaa agtcgttgac
     1921 caccgccgcc gctgaggcgc tgagcgagct ggacaggttg gacgccagga tcgcggccag
     1981 catcaggccg atcaggccgg tgtttcgcgg atagtggttt acgatgaagt gggcaaagac
     2041 ttcgtccggc ttaagcttga cgccagcggg cagcgggtgc tggccgaaga agcaggccag
     2101 ctcgatgccg atgaacagaa acaggccgaa ctgcacgaac acgaccaggc cgctgaccag
     2161 caaggcccag ccggcgtcgc gctgacttcg cgcgctgaga tagcgctgca ccatcgaatg
     2221 gtcggtgccg tgcgtgccga tcgtcaatac cgcgccgccg atcaggctgg cccagaatgt
     2281 gtagggctcg gtgagcgacc aacgcacgtc gagcacctgc catttgtcgg tctcagcggc
     2341 gaagcgggcg atctcgctga agccgcccga gatctgcccg gcgatgacga acacggccac
     2401 gatcccgccc agcacgtaca cgcccacctg gatgcaatcg ttccagatga ccgaccgcat
     2461 gcccccgaca aacgtataga ccgtcgagat gacgcccatc acgacgacgc tgagccagag
     2521 tgggatgccg gcgagctttt gcagcacgat tgccacgaga tacagccgca ggccgtcgcc
     2581 caggttgcgg gcgataagga acagcagcga tgcggcctgc cgcgtcaaac cgccgaaccg
     2641 ctggttgagt acttcgtagg ccgtcagcaa ttggccgcga aagtagagcg gaaacaggac
     2701 atagacaatg atcgcccggc cgatgacgta gccaagtgcg atttgcaaga atttgaaggc
     2761 aacctgccca tagccgtgtc ccggcacgct gagcaccgtc acaccgctcg tttcggtggc
     2821 gacaatggag ccaagaatcg cccaccacgg caagttgcgg tcgccgagca ggaacgcctc
     2881 gacgctcttg ctccgcccgc tgacgagcag gcccaccgcc accgccgtcg cgaaataggc
     2941 caccagcacc aggatgtcga gccagccgag attggccatt cgatcgactt cgagtaacaa
     3001 gtgacaaatg acaagtgacc aatgacaatg aatgccgtcc cagaatacac aggtacgagc
     3061 gcaaaggcat gtcccgcttg acgacggaag cccgcaatcc ggcctcggag cggatcgata
     3121 cgctctcagc cctggaaatc gcccggctga tgaacgccga ggacgcccgc gtcgcggagg
     3181 cggtcggccg agaggcggaa ccgattgccc gggcgatcga gatcatcgcc gagcggctgc
     3241 gggccggcgg ccggctgata tacatcggcg cgggcacttc gggccggctg ggcgtgctcg
     3301 acgcgacgga gtgcccgccg acgttcagca cgccgccgga gatggtcgtc gggctgattg
     3361 ccggcggtta cacggccttg acccgcgccg ttgaaggcgc cgaggatcat ccggaatacg
     3421 ccgtcagcga cttgcggcag attcaattca gccgtggcga cgtgctcgtg ggcatcgcaa
     3481 caagcgggcg cacaccgtac gtcattggcg gcctcaagta tgcccgagaa gtcgaggcgg
     3541 cggcgatcgg tctggcctgc aacgacggct ccgcgctggc cgaattcgcc gacctgatga
     3601 ttacgccggt cgtcggaccg gaggtcatca gcggctcgac gcggctgaag gccggcacgg
     3661 cgacaaagct ggtgctcaac atgctcacga ccggggcgat ggtcctgctc ggcaagacgt
     3721 acggcaactt gatggtcgat ctgaaggcga ccaacacgaa gctcgtcgct cgcacgcggc
     3781 gaatggtggc gatgctgacc ggcgtgagcg aagagaccgc tgaatcgttg ctcggcagtt
     3841 gtggcggcga gctgaagacg gccgtcgtcg cccaccggca cggaattccg gccgaggccg
     3901 cccgcgagat gctgcggcgc gccggcgggc agttgcggat tgctctcgag tcgccgacgc
     3961 atggctgagc accaggaatt cgttatcggc gtcgatggcg gcggaacaaa gaccgcggcc
     4021 tggctggcat cgctcgtgga cgacacgatt ctcggccgcg ggcaggccgg gccgggtaat
     4081 ccgcgggcgg cggggttcga ggtggcgcag gccaacatcg cggcagccgt cgcggcggcg
     4141 tttgacgatg caaaactgtc tcgacaggcg gttgccgccg cctgtttcgg gctggctggc
     4201 gctggccggg aggtcgagca gcagctgatc gccgactggg cgctgggccg cagaatcgcg
     4261 aaaaaagtcc gcgtctgcgg ggacgccgag ctaatcctgg ccgccgcttc gccggagaat
     4321 tgcggtgtgg cgctgatcgc tggcaccgga tcgctggcct ggggccgcaa tcaggcgggc
     4381 gaaacgaccc gctgtggcgg ctggggctat ttgctcggcg acgaggggag cggatatgcc
     4441 atcgccctgg cgggactgcg agcggcgatg cgggccgccg acggccgcgg gccgaccaca
     4501 ggcctgctgc cggctttcct gcgcgagctg ggcgctgcta cggcgccgga gctcatcggc
     4561 aaggtctatt cgcccgagat gacgcgggag cggctggcgg gcctggcgag cgtcgttttc
     4621 gaatatcgca gcaccgacga ggtcgcccag cgaatcgtcg ccgacgccgc gggcgaactg
     4681 gcggcgatgg tcacgacggt tgcaacggag ctgaaactgc cgccgcaagg ctacatgctg
     4741 gcgatctctg gcggcgtact gctgaaccag ctcaattatc tggagcagtt ccttttcacg
     4801 ctgtgggccc acacgcactc gggtgagtcc cagccggctc gctgcgtcaa agtcgaagag
     4861 ccggtgcgcg gcgcggtcgc gctggcccgg ttgctcgctc ggtaggtctc gcctgccggg
     4921 cgggacctgg gagcggccag atcgctgcgc ttccctaagg tcccttccgg cagaagggac
     4981 ctacttcgcc cggcccacgc cgaactggtc gccgctagcc acgggctggc cgccgcgcca
     5041 ttggatttcg accatcttct cgctgtggac ctggccgcct ttctcgtcgg ccagcgcggt
     5101 gacaatcttc ttggtggccg tgtcgatgac ttcgccggtc gacggatatg ccagtttgcc
     5161 gtccaggctg aaagtgatcc agccgggctg ctcgcggagc gaaatgctct ccttctgctt
     5221 cggcggttcg acgctgttgt cgaagacgtg gacctgctgg ttggcggcat cgacgaccca
     5281 gacttccttt tcatcgggcg tcagtccgat gccgtggctg gggcagccgt ggcgcttgac
     5341 catgccgggc ttcacaccgg cgacttccac gcgatgcaac ttttggccgc tcgtcagatc
     5401 gccgatttca aagcccaggc agtcgttgac gcagatgtag gcccgcgtct tcgccgcgtt
     5461 gacagtgaag gggcgaatcg agccggcaaa cgggccggcc ttctgcacga tatcgtgcgt
     5521 cttggtgtcc gcgacgaaaa gcagcggcga cttcaggccg cccaagtaca tccgcgaacc
     5581 gtcgcggctg acgaccgtgt tgtgcgcgcc gctcttggtc tcgatcgacg taatcagctt
     5641 gccgtccgca gcgtcgatta cgttccacgt gtccttctcg aacgacggaa catagatcgt
     5701 cttgccgtcg ggcgtaatcg acatccgatc cgtgccgttg ggcggcgtcg tctcccaaag
     5761 cgtttgctcc gtggccaaat cgacgcagta cagcttcttc gtcgtcgtaa agtagagccg
     5821 cttcgtcgcg gcgcaggcgc agacgccctt gatgttgtct ggcttctcct cgcggctggc
     5881 cggcgtgccg attcgcttga caaacttgtg gccgtggtcg atgtcgaaga ccaggatgcc
     5941 cgcgccgccg aattccaggt agttgcggat gcccggacag acgacgtaca gatatcggcc
     6001 gtcgggcagg gcctcggccg aagccggctg cagcgacaac agggcgagca ccagaccgat
     6061 cgacaggcga atgaaatgag aacgcatggt gacctcgggc agcgggtgta gagccgtgtg
     6121 tttctatttc tgagcaaagt aatcgacgac cagaaccttc tctaccttgg gcagtgccaa
     6181 gcgcacgaag gcctgatggg cgtcgtgcgg cagataggtg tcgcgggcct tggcgtcctt
     6241 gaaggtaacg acgaagcagt gcgtaaaacc ttcgctgcgt ttctcggggc tgacgtcggt
     6301 cccccactcg aacgccgtga tgccgtcgat ctttttcggg agctggccga atccagcgac
     6361 gatctcctgg acctgctctt tggtggcgtc ggccttgaac ttgaacagca cgatatgccg
     6421 cagcggagca gcctctttat cctgggcgag cgcagggctg gtgacgttca aggccagtgt
     6481 cgccaaggcc gcccccagca gggcgaaccc gcaggcgaag atcgttgtcc gggcaagcga
     6541 aaagggcata actggctcct gtcatcttgg gagttgggcg tttggccggt agcgtggtgg
     6601 attgtgcgcc gcgcgtcaat cagggtcgcc tcccgatgta tattccggcc catttcgccg
     6661 ctcccgacca ggagtcactc tacgccgcgg tcgagcggta cagttttgcc accctggttt
     6721 ccttacgccc ctccggcgga atcactgcca gccatttgcc gctgctgctg gagcggcctc
     6781 ggaccgacgg catccagagc ctgcctgctg ccttggtcgg gcatatggcc cgcgccaatc
     6841 cgcagtggcg cgacgcggcg ggccaggaag tgctggcgat cttctctggg ccgcatgctt
     6901 atatctcgcc gacgtggtac gaagccaaac aggtcgtgcc gacttggaac tatgtggctg
     6961 ttcatgcctg cggccagctg gaactcgtcg aagacgcaac tgcagcggag gcaattctcc
     7021 gccgcactgt cgcgctctac gaagcagggc agccgcggcc ctggacgctc gacgaacccg
     7081 ccgaattcgt cgagcgcctc accgctcaga tcgtggcgtt tcgcattccg atcgcccgca
     7141 tcgagggcaa gtggaagctc aaccagaatc atccagccga tcggcgggcc aaggtcattg
     7201 ccgcgctgga gcaacagggc gacgagaaca gccgcgagat cgcccggctg atgcaatcat
     7261 cgtagtcatc tcgctccgcg agatgcggcc tcacgcggag cgtgaggacg actatgagtt
     7321 cgcgggccgc gtgcggttac ggccggactg cttggcttcg cggagggcgg cgtcggcccg
     7381 ctccagtgcg tcctgagcgg ctgctttgtt ggcgggcccg gtgagcacgg cccagcccgc
     7441 gctgagcttc agcggggaac ggttggcggc ggcgtcgccg tcttctccgg cctggatgcg
     7501 gatcaaatcg accacggccg cggcccggtc ggcggcgatg ccctccagga gcacggcgaa
     7561 ctcatcaccg cccagccgcg ccagaaagcc cgctcgccgg acgccgcccg ccagccggtc
     7621 ggcgatccgc cgcaggcaac tgtcgccctc gaggtgcccc tgccgatcgt tgatctcatg
     7681 gaacagatcg acgtcgagca gagccatgca ccaggcgctg ccttcggccg ctcctcctaa
     7741 gcgatcgttc aactgctgct cccacgcgcg gcggttcggc aggccggtca gcggatcgga
     7801 aagcgccaaa tgcgtgagca ccttttcctg ccgccggctg gcctcgcgct gccgccgcag
     7861 cctcacgatc tcggccagca gcaggcaggc cagccgcagc tcgcgcgacg aatggtcctc
     7921 ggggagcgac acgtcggcgg gcaggccgac tcccacagca accattccca tctggccacg
     7981 cgacagccgc tcttcatcga tcgcgatgtt tgcatccgtg agcggctgat cgatgaccag
     8041 cacgtcgatg tcctcagccg gcgcgccgtt ggtcgtcacg acctgacagc gggccgtacg
     8101 gagggcttcg gtccacgcgc gctgccgccc ctcgtcgcgg gtcgccagca ggatgcgcgc
     8161 gggatttctc atactgagac gattatatcg ccgcccgcat attaacccga agcgtaagcg
     8221 aggggcattt tggtgtgtga aacgcggttt ccctcatcgg tgcaccgcga gaggcccaac
     8281 tcggcatcct tgtcgccgag gcggaggctc tctaaaatca ccccaatttt gaacccagcc
     8341 agccggccca ggtgtcgcca tgaacgcctc cactgcggat ttcagcacga ttcccgcggt
     8401 ctggacgcgg cagcacctgc tggatctgga aagcctttcc gccgaagagc tgacctgcct
     8461 gctcgacgcg gcaacggcgt tcaaggacgc cactgacggc tgccggcgga agatatccgt
     8521 ccttagcggc cgcacgctcg ccaacttgtt cttcgaaaac agcacgcgga cccgcaacag
     8581 cttctcgctg gctgcccggc ggctgggggc cgacacggtc gagttctcct cgtcgggcag
     8641 cagcgtcagc aagggcgaaa cgtttatcga cacggccaag aacatcgaag cgatgaacgt
     8701 tgacgcggtc gtgattcgcc accagacgcc ggggacgccg catctgcttg ccaagaacct
     8761 gaattgctcg gtgatcaacg ccggcgacgg ccctcacgag catccgacgc agggcctgct
     8821 cgacatcctc accatccggg agcggcgcgg cacgctggcc gggctgacgg tcgctctggt
     8881 cggcgacatc gcccacagcc gcacggcccg gtcgaacatc tgggggctca agaagctggg
     8941 cgcccacgtc atcgtctgcg gtccggcgac gctcgtctcg cggcgctggg aagagttcgg
     9001 cgtggaggtg gcgtacaacc tcgacgaaat cctgcctcgc tgcgacgtgc tgaacctgct
     9061 ccgcattcaa ttcgagcggc agcatacacg gccgttccca tccgtccgcg agtacgccct
     9121 gctgtatgcg atgaaccgcg agcggctgaa ccgggccaag gacgacatcc tgatcatggc
     9181 cccggggccg atcaaccgcg gagtcgagat cacacccgaa gtggccgacg ggccgcacag
     9241 cgt
//
LOCUS       DASRJB010000826        10947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3618301, whole genome shotgun sequence.
ACCESSION   DASRJB010000826 DASRJB010000000
VERSION     DASRJB010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618301"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFB80_21720"
     CDS             complement(<1..196)
                     /locus_tag="VFB80_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36469.1"
                     /translation="MNATLRLLAVTAVVCSAGISRADSGTVWSLGPGGQAQGQIGRWE
                     QQGDRVRFQWAASSQGFDVQF"
     gene            complement(325..1392)
                     /locus_tag="VFB80_21725"
     CDS             complement(325..1392)
                     /locus_tag="VFB80_21725"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZN36470.1"
                     /translation="MATTTAPPAPTIERVDVAHQPSPDSGCGFPRLTGVQILACGGYA
                     PPRVVTNEDLAQHGYDAQWIVQRTGILARRWAEPGVAASDLACEAAKRCLAQAGVRAD
                     ELDLIVLATMTPDSPIPSTACQLQRRLGGRAAAMDVGAACAGFMFALVTGMQFVKTGC
                     ARRVLVVGSDVMTRTINPADKKTFPLFGDGAGAVLLGAGSGDQGLLAYSLGSDGSGAD
                     LLCIRGGGSREPLTPETIAAGRQFIHMEGRPVFKWAVNLLADSIRGAIAAAGLTTGDL
                     SLVVLHQANRRILDAAADALGIDRDKLLVNLDRYGNTSAGSIPLALSEALAAGRLRRG
                     DHILLSGFGAGLAWGTAVLRW"
     gene            1540..2964
                     /locus_tag="VFB80_21730"
     CDS             1540..2964
                     /locus_tag="VFB80_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36471.1"
                     /translation="MSHKSHRSKPLRLLIEPVVSVAEALAERVRVELPTHDGLGKAAA
                     GVAAAARQAELVSRRMSRVFSLHRLPAVFLAAALVCLVIWIYWRFFHVSTLSLALPDR
                     DAMALRDAVAGRQRVNFRREIVPGSREAVERVSAGSVDLAFVQGGLEIPHELPRLELP
                     SPEVVLWLVREGVAGPHEVKKVLTSTSGEGSHSVAQLFLAAWHTRGQTEFDHSWREVT
                     ANSQFEIAKDIDAAFIVKDPADEKTALGIQRLYAAGFRLRSPELGARGARHEFLKSIA
                     IPPRYLVSDPEIPAEPIATYSVATYLVARRGMTPRLLAQAAHVLDAAPLKIADAGFDP
                     TTTDASEIFQGIEAFMGIIVNIGLAFLALLGLDVMAYRKQFHELNSLVSLLSMLQSNK
                     DVLGLKDAGQRAENLLYLSLVSDLLGVISMISSYYTQENSSLLFNNLSEVIHQRCDGL
                     KINIQLKVLHATVDLPQPVSAQQE"
     gene            complement(3019..4044)
                     /locus_tag="VFB80_21735"
     CDS             complement(3019..4044)
                     /locus_tag="VFB80_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36472.1"
                     /translation="MSAVRQALAGSGEQVVEIGPRIEGLIRSWQCGQFGLSLRALLLY
                     AQWGDWNERRPGVVVAGEDVLLGVGLYHRPDGWHLHAVCPQGGQRAPRIRTIARQLLA
                     AGLVDRVYVRHLTPSAEREFLAAGFVPPERYPWLPDSPAEDETHPHRLVSLPEIMSGR
                     QRALRLAFRRFANFLRRSQALFWLVPLSPDSYPLAAAVVRDHLQVTGKPLAAHDCEIL
                     FRLPLPNSDPYVSLLCWLQTGSARIPVGVFLGEQTGKTSGALYVNVARRDPAVLEMLG
                     CQDATGFSAIAAFLHAQLFATLLVRGWQKLDLGGSETPSLDRFKQTIGARPLVTKWVV
                     LTRAELT"
     gene            4168..5292
                     /locus_tag="VFB80_21740"
     CDS             4168..5292
                     /locus_tag="VFB80_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZN36473.1"
                     /translation="MSDLLIRPVENAADQDAFLRLPWRIYRGDPNWVPPLISVQAELA
                     GFRRHPFYEAAKSRCFLAVRNGEACGRIAAIVNDAHLRLHNDGCGFFGFFECVNDGEV
                     AKELFAAARAWLQQQGCRMVRGPFSPSVNYESGLVVTGCDRPPAFLAAYSAPYYAGLV
                     ESAGFRPKWELMAFEVERSMYPARDPKLEFICEESLRRSGAKLRQFDRWQMSRDIRRF
                     FDLYNRTLDNIPFFTPMTAREIEAAIGWLKMLTAPELTAIAEVDGQPVAALYGLPDYN
                     PIQRTLNGRMGPLGALRWWWNRRRFTRARFFSINVLPEFHAWGLGVALFRNVLRDGLA
                     RGLQSAELSWVLESNQLSRGSLERGGAKLTKTYRIYEAEL"
     gene            5379..5843
                     /locus_tag="VFB80_21745"
     CDS             5379..5843
                     /locus_tag="VFB80_21745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN36474.1"
                     /translation="MAHGEVSQTIPASCEEVFDLLHDYERRLEWDSLLSEARLTGGHT
                     RAGLGATSVCVGRRSLGGVALETEYVAFERPRLAAVKMINRPPLFASWAASIKHEPAA
                     GGSRVAYTWTFTARPRWLAWLLEPILNRVFRRETRRRLAALAAHFAERRGCG"
     gene            complement(5840..7285)
                     /locus_tag="VFB80_21750"
     CDS             complement(5840..7285)
                     /locus_tag="VFB80_21750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZN36475.1"
                     /translation="MKRTTSKLSQNYNRRLMLVECLERRAMMTNNPPEVILTLDPSLQ
                     FGEPHEGSNQVSSDHIADWRLKIDFQDDEGGTFDLVFDLNGNGMFGESGEPPTKTITV
                     PARPQGQYDTYTLDAIQWPEIVGLQMGDTVDGSVDFTMAVRVFQANILVGADTDMLTI
                     KNSLPFFQGTIVPVQAAAAQSAGCGGAVAPVTLSGSFVEYGVNDAVTLRINWGDGNVE
                     TFVHSNLSNDEVSFSKQHTYAASGNYTIKWDVWDDEDQALLSESPASQGQFTNFPVTG
                     GGPAGPSVCLAGGVLTVTGSAGNDSAMITQPNGVIRVESSFFPTTEFPAANVQNIVAL
                     LGAGNDMLVVNASKPLVAVGGDGSDVINGGNARSILIGGAGNDLLIGGSAQDILVGGA
                     TTHDSNAVALLLLLAEWNSAHSFTDRVNNLTNGSGSVQGLNETPSGSFFLTSLGDDQA
                     VDILVGAGSMDWIIRHAGDISMGLADFLTPP"
     gene            complement(7531..9273)
                     /locus_tag="VFB80_21755"
     CDS             complement(7531..9273)
                     /locus_tag="VFB80_21755"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN36476.1"
                     /translation="MKSILVSVSSACLAVISFAATLPAAELATQLRRPVGLAQSEDGR
                     WLYVANRDSGSISVIDLSSREVVAEHAIGKRLSDLAALPDFGRLLAVDEAAHELLLLD
                     PDGDRVRVVQRLPVSPYPTSIHVAAGGREATIASLWSRRLSFVELGAEAKVAAALDLP
                     FAPRCQVRLPAKDRLLVADSFSGKLAIVELSSRSLVQTVAFPGHNIRGLGVSPDGQML
                     MAAHQMLNELAHSIQNDIHWGLLMSNDLRWLKVDSVLAGGKQLYYGAHMHPLGEANRG
                     GGDPGGLDVAPDGTVVVAISGVSEVALGKEGDFSMRRVKVPRRPTAVRIAKGGRQAFV
                     ASTLDDSISILDLAEKKLVGTIKLGPAPELSLAQQGELLFFDARLSHDGWMSCQSCHT
                     DGHANGQMNDNFSDRSFGAPKRVLSLLGVRDTLPLAWSGQVQTLERQIHNSVESTMQR
                     DDAFPPDQARAIAAYVQTLELPPSLDALRGTADRAAIARGKLVFERRNCAQCHPAPTY
                     TSPGAYDVGLTDAEGNKKFNPPSLRGLSHRGPFFHDNSAAKLEDVFLTHGHPSDSAFS
                     AAEVRDLVAFLQNL"
     gene            complement(9446..>10947)
                     /locus_tag="VFB80_21760"
     CDS             complement(9446..>10947)
                     /locus_tag="VFB80_21760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36477.1"
                     /translation="RGGVRGVSTGQERLNLDETTIAQTFKAAGYATGAFGKWHNGLQA
                     PYHPLDRGFDEYYGFCSGHWGEYFDPPLEHNRQFVRGRGFITDDLTERSMQFIEQRRD
                     KPFFCYLALNTPHSPMQVPDSYWDRFKNKELKLRHYQPGQEEIEHTRAALAMVENIDW
                     NVGRLLAKLEELKLAENTIVIYFSDNGPNGWRWNDGMKGRKGSTDEGGIRSPLLVRWP
                     GQIKPGTKVTQIAGAIDLLPTLADLAGVKVASAKPLDGRSLKPLLTGMTSPWPERSLV
                     TFYGGRLSLRTQQYRLDAAGGLFDMVADPGQKRDIAKEQPQIAAQLSAEAAKWRAELA
                     PGVGKDERPYTVGYASFTPLPARDGVPHGNVKRSASAPNCSYFTNWIGSAEDRITWEV
                     EVGQAGRYEASVHYACPAADIGSTIELSLGQRRLQAKVTQPHDPPLYGEAHDRVPRRG
                     ESYMKDFKPLVLGVLELPAGRGQLALKALNVPGKQVMEVRYVTLTRLDK"
BASE COUNT         1835 a         3654 c         3586 g         1872 t
ORIGIN      
        1 gaaactgcac atcgaatccc tgggaggagg cagcccactg aaaccggacg cggtcgcctt
       61 gctgctccca cctgccaatt tgcccctgcg cctgcccgcc cggccccagg ctccaaacgg
      121 tccccgagtc ggcccgggaa attcccgctg agcaaacgac cgccgtaacg gcgagtaatc
      181 gcaacgtggc gttcatagtg cttcccccga cttgagtcgc ggatcgagca cggtaatgtc
      241 cgtacgcttc aggtcataag acgcaaacgg cgttccgatc cttggattct accacgcacg
      301 cgccgggaaa atggagcgat tgcgctacca ccgcagcacg gccgtgcccc aggccaggcc
      361 ggcgccgaag ccggagagca ggatgtggtc gccgcggcgg aggcggccgg cggcgagggc
      421 ctcgctgagg gccagcggaa tgctgccggc cgaggtgttg ccgtagcggt cgagattgac
      481 gagcagcttg tcgcggtcga ttcccagggc atccgcggcg gcgtcgagta tgcggcggtt
      541 cgcctggtgc agcaccacca gcgacaaatc gccggtcgtc aggccggccg ccgcgatcgc
      601 gccgcgaatc gaatcggcca gcaggttcac ggcccacttg aagaccggcc ggccttccat
      661 gtgaatgaac tggcggcccg ccgcgatggt ttccggcgtg agcggctcgc gcgagccgcc
      721 gccgcgaatg cagagcaggt cagcgccgct gccgtcggag ccgagcgaat aggccagcag
      781 tccctggtcg ccgctccccg cgccgagcag cacagccccc gcgccgtcgc cgaacagcgg
      841 aaaggtcttc ttgtccgccg ggttgatcgt ccgcgtcatc acgtcggagc cgaccacgag
      901 cacgcgccgg gcacagccgg tcttgacgaa ctgcattccg gtcaccagcg cgaacatgaa
      961 tcccgcgcaa gccgcgccga catccatcgc cgccgcccga ccgcccaggc gcctttgcag
     1021 ctggcaggcc gtcgaaggaa tcggactgtc gggcgtcatc gtcgccagca cgatcagatc
     1081 gagctcgtcg gcccgcactc cggcctgagc caggcagcgc ttggccgctt cgcaggccag
     1141 gtcgctggcg gccacgccgg gctcggccca ccgccgcgcg agaatgcccg tccgctgcac
     1201 gatccactgc gcgtcgtagc cgtgctgggc caggtcctcg ttcgtcacga cccgcggcgg
     1261 agcgtacccg ccgcaggcca gaatctgcac accggtcagc cgcggaaagc cacaaccgct
     1321 gtccggcgag ggctgatgcg ccacatcaac gcgctcgatc gtcggtgcag gaggggcggt
     1381 cgtcgtggcc atgcaattcg agtggcggcg aaagatcgaa tgttataacc gcagtcttcg
     1441 ccgccggata gttacaagcc gctggccgca gggtacaaca gtcggctgcc ggcgaacagg
     1501 gcaaatcgcc caccgccggt cgccgttcga gatcatgacg tgagccacaa gtctcatcgc
     1561 agcaagcctt tgcggctgct gatcgagccg gttgtgagcg tggccgaggc gctcgccgag
     1621 cgggtgcgcg tcgagctgcc gacgcacgac ggcctcggca aagcagccgc gggcgtggcg
     1681 gccgccgcgc ggcaggccga gctagtttcg cgccgcatga gccgcgtctt cagcctgcac
     1741 cggctgccgg cggtgtttct ggcggcggcg ctggtttgcc tggtgatctg gatttactgg
     1801 cggttctttc acgtctcgac gctctcactg gccttgcccg atcgcgacgc gatggcgctc
     1861 cgcgacgcgg tcgccgggcg gcagcgggtc aactttcgcc gcgagattgt tcccggctcg
     1921 cgcgaggccg tcgagcgcgt cagcgcgggt tcggtcgatc tggcgtttgt gcagggcggc
     1981 ctggagatcc cgcacgaatt gccgcggctg gagctgccca gccccgaggt cgtgctttgg
     2041 ctcgtgcgcg aaggagtggc cgggccgcac gaagtgaaga aagtcctcac ctccacgtcc
     2101 ggcgaaggca gccactcggt ggcgcagttg ttcctcgcgg cgtggcacac tcgcggccag
     2161 accgagttcg accactcgtg gcgcgaggtg accgccaatt cgcagttcga aatcgcgaag
     2221 gacatcgacg cggcgttcat cgtgaaggac cccgcggacg aaaagacggc cctgggcatc
     2281 cagcggctgt atgcggccgg gtttcgcctc cgctcgccgg agctcggcgc gcggggggcg
     2341 cgtcacgagt ttctaaaatc cattgccatt ccgccgcggt acctggtttc cgacccggag
     2401 attccggccg agccgatcgc gacctattcc gtggcgacgt atctggtggc ccgccgcggg
     2461 atgacaccgc gtctgctcgc gcaggcggcg cacgtcctcg acgccgcgcc gctcaagatc
     2521 gccgacgcgg gcttcgaccc gaccacgacc gacgccagcg agatctttca gggcatcgaa
     2581 gccttcatgg ggatcatcgt caacatcggc ctggcgtttc tcgcactctt gggcctggac
     2641 gtgatggcct atcgcaagca gttccacgaa ttgaactcgc tggtcagcct gctcagcatg
     2701 ctccagagca ataaagacgt gctgggcctc aaggacgcgg gccagcgggc cgagaatctg
     2761 ctctacctca gcctggtcag cgacctgttg ggcgtgatca gcatgatcag cagctactac
     2821 acgcaagaga actcctcgct cctgttcaac aatctgtccg aggtcatcca ccagcgctgc
     2881 gacggcctga agatcaacat ccagctcaag gtcctgcacg ccacggtcga tctgccgcag
     2941 ccagtgtccg cgcagcagga gtagacgaat tcacccgctt agccccggcc cccaggccgg
     3001 ccgcgccgcg tggccggcct aagttaattc cgcccgcgtc aacaccaccc acttcgtcac
     3061 cagcggccga gcgccgatcg tttgcttgaa acggtcgagg ctgggtgttt cggagccgcc
     3121 gaggtcgagc ttctgccagc cgcggaccag cagcgtcgca aagagctgcg catggaggaa
     3181 cgccgcaatg gcgctgaacc cggtggcgtc ctggcaaccc agcatctcca gcacagccgg
     3241 atcgcggcgg gccacgttca catagagtgc gccgctcgtt ttgcccgtct gttcgcccag
     3301 gaagacaccg accggaattc tggcggaacc cgtctgcaac cagcacagca gcgacacata
     3361 cggatcactg ttgggcagcg gcagccgaaa caggatttcg cagtcgtgtg ccgcgagcgg
     3421 cttccccgtg acctgcaagt ggtcgcgtac gacagcggca gccaagggat agctatcggg
     3481 cgaaagcggt accaaccaga acaaggcctg cgagcgacga aggaaattgg caaatcgtcg
     3541 aaatgccaac cgcaatgccc gttggcgacc ggacatgatc tcgggcaagc tcaccaggcg
     3601 atgcggatgc gtttcgtcct ccgcgggcga atccggcagc cacggatagc gctcgggcgg
     3661 cacgaatccg gcggcgagaa actcacgctc cgcggacggc gtcagatgcc gcacatagac
     3721 gcgatcgacc agtcccgcag ccaagagctg ccgcgcgatc gtgcgaatcc gcggcgcgcg
     3781 ttgcccgccc tgcggacaga cggcgtgcaa gtgccagccg tcgggccggt ggtaaaggcc
     3841 gacgcccagc agcacgtctt cgcccgcgac gacgacgccc ggccgccgct cgttccaatc
     3901 gccccactgc gcatacagga gcagcgcccg caacgacagg ccgaactggc cgcactgcca
     3961 cgagcgaatg aggccttcaa tccgcgggcc gatctccacg acttgctcgc cgctgccggc
     4021 cagggcctgg cgcacggcgg acattcggtc gcgatcggtg agttccaatt ttctgcctcg
     4081 cgctggcaat tggctgctcg tgggccatcc cttatagtcc aggccgccgc cgtcggtctg
     4141 cccccttacc gccacaactc ctgccgcgtg tccgacttgc tcattcgccc cgtggagaac
     4201 gcagccgatc aggacgcgtt tctgcggctg ccgtggcgga tttaccgggg agatcccaac
     4261 tgggttccgc ccttgatctc cgtgcaggcc gagctggccg gcttccgccg ccatccgttt
     4321 tacgaagcgg ccaagagccg ctgctttctc gcggttcgca atggcgaagc gtgcggacgg
     4381 attgccgcga tcgtgaacga cgcccacctg cggctgcaca acgacggctg cggattcttc
     4441 gggtttttcg agtgcgtgaa cgacggcgaa gtcgcgaagg aattgttcgc cgccgcgcgg
     4501 gcctggctcc agcaacaggg ctgccgaatg gtgcgcgggc cgttcagccc gtcggtgaac
     4561 tacgaatccg gactggtcgt caccggctgc gaccggccgc cggcatttct ggccgcctac
     4621 agcgcgccgt actatgcagg cctcgtcgag agcgcgggct ttcgcccgaa gtgggagctg
     4681 atggcgttcg aggtcgagcg gagcatgtat ccggcacgcg atccgaagct ggagttcatc
     4741 tgcgaagaat cgctccgccg cagcggcgcg aagctgcggc aattcgaccg ctggcagatg
     4801 tcccgcgaca tccgccgctt cttcgacctc tacaaccgca cgctcgacaa tatcccgttc
     4861 ttcacgccga tgacggcccg cgagatcgaa gccgcgattg gctggctgaa gatgctcacc
     4921 gccccggagc tgaccgcgat cgccgaggtg gacgggcagc cggtggcggc gctgtatggc
     4981 ctgccggact acaatccgat tcagcggacg ctgaacgggc ggatgggccc gctcggcgcg
     5041 ctgcgctggt ggtggaaccg ccggcggttc acgcgggccc gcttcttcag catcaacgtg
     5101 ctccctgaat ttcatgcctg gggtctgggt gtggccttgt tccgcaacgt gctccgcgac
     5161 ggcctggccc gcggcctcca gagcgccgag ctctcctggg tcctggaatc gaatcagctc
     5221 tcgcgcggct cgctcgagcg gggcggcgct aagctgacca agacctaccg gatttacgag
     5281 gcggagcttt gacgggctat tcttcggttc tttggttctt cggttcttgg ttcttcttcc
     5341 cccgttatgc accacctcgg cattgcggta agcagctcat ggctcacggc gaagtctcgc
     5401 agaccatccc cgccagctgc gaggaggtct tcgatctcct gcacgattac gagcggcggc
     5461 tggagtggga ttcgttgctc tcggaggcgc gattgaccgg cgggcacacg cgggccggcc
     5521 tgggcgcgac cagcgtctgt gtcggccggc gctcgctcgg gggtgtggcg ctggagactg
     5581 agtatgtcgc gttcgagcgc ccgcggctgg cggccgtgaa gatgatcaac cgccccccgc
     5641 tgttcgccag ctgggcggcg tcgatcaagc acgagccggc cgcaggcggc tcgcgggtcg
     5701 cctacacctg gacgttcacg gcccggccgc gctggctggc gtggctgctg gagccgatcc
     5761 tcaatcgcgt ctttcgccgc gagacccgcc gccggctggc ggcgctggcg gcgcactttg
     5821 ccgagaggcg cgggtgcggc tagggcggag tcaaaaagtc ggccaggccc atgctgatgt
     5881 cgccagcgtg gcggatgatc caatccatgc tgccggcgcc caccaggatg tccacggcct
     5941 ggtcgtcgcc gagcgaagtc aggaagaaac tgccgctggg cgtctcgtta agcccttgca
     6001 cgctgccgct gccgttggtc aggttgttca cgcggtcggt gaacgagtgg gccgagttcc
     6061 attccgccag caggagcaac agggccacgg cgttgctgtc gtgcgtcgtc gcgccgccga
     6121 cgaggatgtc ctgggcgctg ccgccgatca gcaagtcgtt gccggcgccg ccgatcagga
     6181 tgctccgcgc gttgccgccg ttgatcacgt ccgaaccgtc gccgccgacc gcgaccagcg
     6241 gcttgctggc gttgactacc agcatgtcgt tgccggcgcc gagcagggcc acgatattct
     6301 ggacgttggc cgcggggaat tcggtcgtgg ggaagaacga cgattccaca cgaatgacgc
     6361 cgttgggctg agtgatcatg gccgaatcgt tgccggcaga gcccgtgact gtcagcacgc
     6421 cgccggccag gcagacgctc ggcccggcgg ggccgccacc ggtaaccggg aagttggtga
     6481 attggccctg gctggccggg ctctccgaaa gcaacgcctg atcctcatcg tcccacacgt
     6541 cccacttgat cgtgtagttc ccggacgcgg cgtaggtgtg ctgcttggaa aaggaaactt
     6601 cgtcgttgct caggttggaa tggacgaacg tctcgacatt gccgtcgccc cagttgatcc
     6661 gcagcgtcac ggcgtcgttg accccgtact cgacgaacga gccggagagc gttacgggag
     6721 cgaccgcgcc gccgcagcct gcgctttggg cagccgcggc ttgcacgggc acgatagtac
     6781 cttggaaaaa cggcaggcta ttcttgatcg tcaacatgtc cgtgtctgcg ccgaccagga
     6841 tgtttgcttg gaagacgcgg accgccatcg tgaaatccac cgagccatcg accgtatcgc
     6901 ccatttgcag gcccacgatc tcgggccatt ggatcgcatc cagcgtgtac gtgtcgtact
     6961 ggccctgcgg gcgggccggg acggtaatcg ttttcgtcgg cggttcgccc gactccccaa
     7021 acatgccatt cccgttcagg tcgaaaacga ggtcgaaggt gcccccttcg tcgtcctgaa
     7081 agtcgatctt gagccgccaa tctgcaatgt gatcggagct cacctgattg ctgccctcgt
     7141 gtggctcgcc gaactgcagg ctgggatcga gggtaaggat gacctcaggc ggattgttgg
     7201 tcatcatcgc ccgccgctcc aggcactcca cgagcatcaa gcggcggttg tagttttgcg
     7261 acagcttcga cgttgtgcgt ttcatttgat ccctcaagcg acaggaacag attggcgttc
     7321 gtctatctaa tcggcagcgg cgaaaatctc cgggcaaagt tagttgacag ccgcaaccgg
     7381 cacgacaacc caatcagcca cccgtcgcct ggattgggag gctagttagt ccagggaaga
     7441 taagcaatat cacgcctacg cgagctggca actttgcggt aaggcgcaaa agattttgac
     7501 gattttcgca aagccagctg gggcaacgac ttataaattc tgcaaaaaag caacaaggtc
     7561 gcgaacttcg gctgctgaga aggccgaatc gctcgggtgt ccgtgcgtca gaaagacatc
     7621 ctcgagcttc gccgcgctgt tgtcgtggaa aaaggggccg cggtgcgaaa ggccgcggag
     7681 gctgggcgga ttgaacttct tgttgccctc ggcgtcggtc agtccaacgt cgtatgcgcc
     7741 cggcgaggta taggttggcg cggggtggca ctgggcgcag ttgcgccgct cgaagaccag
     7801 cttgccgcgg gcgattgccg cgcggtcggc agtgccgcga agcgcgtcca ggctgggcgg
     7861 aagctccagc gtctgcacgt aggccgcgat ggcccgcgcc tggtcgggcg gaaacgcatc
     7921 gtcgcgctgc atcgtgctct cgaccgagtt gtgaatctgc cgctcgagcg tctgcacctg
     7981 cccgctccag gccaaaggca gcgtgtccct gacgcccaag agcgagagca cgcgcttggg
     8041 cgcgccgaac gagcggtcgc tgaagttgtc gttcatctga ccgttggcgt ggccgtcggt
     8101 gtggcaactc tggcagctca tccagccgtc gtgcgacaaa cgggcgtcga agaacagcag
     8161 ctccccctgc tgcgcgagcg acagctcggg cgcggggccg agcttgatcg tgcccacgag
     8221 cttcttctcg gccaggtcga ggatcgagat ggagtcgtcg agcgtgctgg cgacgaacgc
     8281 ctgccggcca cccttcgcaa tgcgaacggc ggtgggccgc cgcgggacct tgacccgccg
     8341 catgctgaag tcaccctctt tgccgagcgc gacttcgctc acgccggaga tcgcgacgac
     8401 cacggtgccg tcgggcgcga cgtcgaggcc gccgggatcg cccccgccgc ggttggcctc
     8461 gcccagcggg tgcatgtggg ccccgtaata cagctgcttg ccgccggcca gcaccgaatc
     8521 gaccttcagc caccgcaggt cgttcgacat caacaggccc cagtgaatat cgttttgaat
     8581 cgagtgggcc aactcgttca gcatctggtg tgccgccatc agcatctgcc cgtcggggct
     8641 gacgcccagc ccgcggatgt tgtgcccggg gaacgcgacg gtctggacca gactccgcga
     8701 cgagagctca acaatcgcca gcttgccgct aaatgagtcg gccacgagca gccgatcctt
     8761 agcgggcagg cggacctggc agcgcggcgc aaagggcaga tcgagcgcgg ccgcaacttt
     8821 ggcctccgcg ccgagctcga caaacgagag ccgccgcgac cagagcgagg cgatcgtcgc
     8881 ctccctgccg ccggctgcga cgtggatcga cgtgggatac gggctcaccg gcagccgctg
     8941 cacgactcgc acgcggtcgc cgtccggatc gagcagcagc agttcgtgcg cggcctcatc
     9001 gacagccagc aggcggccga aatccggcag cgcggccagg tcggacagcc gcttgccgat
     9061 cgcgtgctcg gccacaacct cgcgcgagct caagtcgatg accgaaatgc tgccgctgtc
     9121 gcgattggcg acgtacagcc agcggccgtc ctccgactgg gccagcccca ccggccgccg
     9181 tagctgcgtg gccaattccg ccgccggcag agttgccgcg aacgaaataa cagccaggca
     9241 ggcggaagat acgctgacga gaatcgactt catgacagca attggcagga caagcggcgg
     9301 cgaattcacc caatgtgcca gaggttccat attcgagcaa ggtaaatccc ttccaccgcc
     9361 ttgcatctct gcgcctccgc gtcctctgcg actttgcgtt aaatgcgcga accgtcgcgc
     9421 cccggccccg cgtcgaattg aatggctact tgtcgagccg cgtgagcgtc acgtagcgga
     9481 cctccatcac ctgtttgccg ggcacattca gcgcctttag cgcgagctgg ccccggccgg
     9541 caggcaactc gagcacgccc agcacgagcg gcttgaagtc cttcatgtac gactcgcccc
     9601 gccgcggcac gcggtcgtgc gcttccccgt acagcggcgg atcgtgcggc tgcgtcacct
     9661 tcgcctgcag ccgccgctga ccgagcgaca gctcgatggt cgaaccgata tcggcggccg
     9721 gacaggcgta gtgcacgcta gcctcgtaac gccccgcctg gccgacctcg acctcccagg
     9781 tgatccggtc ctcggcgctg cctatccagt tcgtgaaata tgagcaattc ggggcggagg
     9841 cgctccgctt gacgttgccg tggggcacgc cgtcgcgggc cggcagcggc gtgaacgagg
     9901 catagccaac cgtgtacggc cgctcgtctt tgccgacgcc cggcgcgagc tccgcccgcc
     9961 acttcgctgc ctccgccgaa agctgcgcgg cgatctgcgg ctgctccttg gcgatgtcgc
    10021 gcttctggcc ggggtcggcg accatgtcaa acaagccgcc ggcggcatcg agtcgatatt
    10081 gctgcgtccg caggctcagc cgcccgccgt agaacgtgac tagcgagcgc tcgggccacg
    10141 gggaagtcat gcccgtgagc agcggcttca ggctgcggcc gtccagcggc ttggcgctgg
    10201 cgactttcac tcccgccagg tcggccagcg tcggcagaag gtcgatcgcg ccggcaattt
    10261 gcgtgacctt ggtgcccggc ttgatctggc cgggccagcg gaccaggagc ggcgagcgaa
    10321 tgccgccctc gtcggtcgag cccttgcggc ctttcatccc atcgttccag cgccagccgt
    10381 tggggccgtt gtcgctgaaa tagatcacga tcgtgttctc ggccagcttc agctcctcga
    10441 gcttcgccag caatcggccg acgttccagt cgatgttctc gaccatcgcc agggccgccc
    10501 gcgtgtgctc gatctcctcc tgtcccggct gatagtgccg cagcttcagc tccttgtttt
    10561 tgaagcggtc ccagtacgaa tccggcacct gcatcggcga gtggggcgtg ttcagcgcga
    10621 gatagcagaa gaacggcttg tcgcggcgct gctcgatgaa ctgcatcgac cgctcggtca
    10681 ggtcgtcggt gatgaagccg cggccgcgga cgaattgccg gttgtgctcc agcggcggat
    10741 cgaaatactc gccccagtgg ccggagcaga agccgtagta ttcgtcaaag ccgcggtcga
    10801 gggggtgata gggggcttgc aggccgttgt gccatttgcc aaaagccccc gtggcgtagc
    10861 cggcggcttt gaacgtctgg gcgatcgtcg tttcatcgag gttcagccgc tcttgcccgg
    10921 tcgagacgcc gcgcactccg ccgcggg
//
LOCUS       DASRJB010000827         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1932243, whole genome shotgun sequence.
ACCESSION   DASRJB010000827 DASRJB010000000
VERSION     DASRJB010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932243"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFB80_21765"
     CDS             complement(<1..306)
                     /locus_tag="VFB80_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36478.1"
                     /translation="MILADLVLGAREWLGAAAALAAVGLAVLLWAYSRSGSQAWVRVL
                     AGLLKAAGIALLAICLVEPMFRGTRPKPGMNLFLVVADNSRSLQLSDKGSRQSRGQLF
                     "
     gene            complement(303..4304)
                     /locus_tag="VFB80_21770"
     CDS             complement(303..4304)
                     /locus_tag="VFB80_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36479.1"
                     /translation="MNQQRLWFELDRVASRYRLLRFWLALAAAWLVAAAAGLALWSIR
                     GALGAGPSVSVLLACLVGAAIAVAGIWLAATSARNYGWLARRIEAAFPELRTCLLAAV
                     EQKPDLPDGRFGYLQSSVIHQALYHADRNAWTEVVPMKRIALAAAANVATFAMFVAVI
                     AVIALSAGPAAKSAALLANAAASSVSARSFAVTVEPGNTEVERGTSLLVLARITGAMP
                     AEATLVFRTEAGEESRLPMSPSLDDPVFGGRIPLVDAPLSYHVELGGQNTPTYQVTVF
                     EYPRLEKADAKLVFPAYTQLPEKLVQDVRTVSAVEGTELTLMCYLNKAVASATLTEDQ
                     QQPIAMAAATPPALPVADMADPKTRSSRATQYFYTAQIKCDRSRKLKLELVDEAGRKN
                     PKPIPFTINVLPNQPPNLKPTFPARDLEVSALEELDVRATVFDDFGLVRAGLAYSING
                     RAAGDLVVSENGAAKQKHELAQQIKLEDLKAEPDQLLSYHWWAEDHAPDGSIRRVMGD
                     MYFAEVRPFEEIFRQGQQPAGGEQQQQQQQQQGQGQNARDAQQLAQLQKEIINATWKL
                     IRRETGSKPTDALKSDAEQVRTSQESAIEQATALAERLTDEQSQEHVESVLKSMATAV
                     EHLKAAEEGPQAAPLTPAMAAEQAAYQSLLKLRAREHEVVRQQQRQRGQQSQQQQRGA
                     RSQQQQQQLNQLQLRNDENRYQTQRAAQAQQQETPEERENRQVLNRLKELARRQHDLN
                     ERVKELQSALEEARTEAQKEEIRKQLKRLQEEQQQILQDTDELQNRMDAPENQERMTE
                     ERQQLEETREQVRRASEALNEEQVTRAAASGTRAEQQFEDLRNEFRRRASGRFNEEMR
                     QMREAARELDEKQQELSQRMAGEERPNEKSKTLRDSGERERIAEELSQQRQRLGNIQE
                     QMREVIQQAEETEPLLSERLYDTDRNLQQQTPERALESAERSLRQGLLNDARRQQEPA
                     GQSIRQLREGIDRAAEAVLGDDTEALRRARQELQDLARELNDEINRNNPNRQPQEGQP
                     QQGEQQPGQENGQKGQGQKGQPQQGEGQKGEGQKGQGQKGQGQKGQKGQGGQPGENQA
                     GQPQEGQPQDGQEGQKGQKGEGQKGQGQKGQKGQGQKGQKGQGGQGGQPGENQEGQPQ
                     DAQAQEGQPQEGQPQQGQRGQKAGQRGQPGQRGGPRRLADGSPNRGGTGPFEDYTPRD
                     LFSPNAGGQEFREWSDRLRDVEEMVDDPELRAEAARIRERARSLRAEAQRHSAEPNWE
                     LVQVQVAGPLVQLRDRVSEELLRRTSQQAIVPLDRDPVPPQFTEKTRRYYERLGSGTS
                     GAAAADEKPQERP"
     gene            complement(4301..>5110)
                     /locus_tag="VFB80_21775"
     CDS             complement(4301..>5110)
                     /locus_tag="VFB80_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36480.1"
                     /translation="ELLPAKAEDDTNQDKFLLLGEIDFSHPLFAPFAGPRYGDFTGIH
                     FWRQRSLKLKEGASSHAVARFDDGQLAIVERTIGTGRALVFASGWNPDDSQLALSNKF
                     VVLVSGILDRACGTTEQLAGAAVGQSVPLPAARRMAAIVNKPDGHDVKLPADAASFSE
                     TDQPGIYRANLAAEEARFAVNLAASESNTAPLEIDQLQQLGVRLSASLTRAQRLSEMR
                     QKRDTELESQQKLWRWIIVGTLGVLVFETFWAGRAARQIQAAATAAQESAS"
BASE COUNT          634 a         1809 c         1685 g          982 t
ORIGIN      
        1 aaacaattgc ccgcgcgact gacggctgcc cttgtccgaa agctgcaagc tgcggctgtt
       61 gtcggcgacg accagaaaca ggttcatgcc gggtttcggc cgcgtgccgc gaaacatcgg
      121 ctcgaccaag cagatcgcca aaagtgcgat ccctgccgcc ttcagcaggc cggccagcac
      181 gcggacccag gcctgcgagc ccgagcgcga gtaggcccac aacagcacgg cgaggcctac
      241 ggccgccagg gccgccgccg cgccgagcca ctcgcgagcg ccgagaacca ggtcggccag
      301 gatcatggac gctcctgggg cttttcgtcc gctgcagcgg cgccgctggt gccgctgccg
      361 agccgctcgt agtagcgccg cgttttttcc gtgaactgcg gcggaaccgg gtcgcggtcg
      421 agcggcacga tcgcctgctg cgacgtgcgg cggagcagtt cttccgacac gcggtcgcgg
      481 agctgcacga gcgggccagc gacttgcacc tggaccagct cccagttcgg ctcggccgag
      541 tgccgctggg cctcggcccg cagcgagcgg gcccgctcgc ggatccgcgc cgcctcggct
      601 cgcagctctg gatcatcgac catctcttcc acgtcgcgca gccgatccga ccactcgcgg
      661 aactcctggc cgccggcgtt tggagagaag aggtcccgcg gcgtgtagtc ctcgaacggt
      721 ccggtcccgc cgcggttggg gctgccgtcg gccagacgcc gcggcccgcc acgctgtccc
      781 ggctggccgc gctggcccgc cttctggccg cgctggccct gttgaggctg cccttcctgc
      841 ggctgaccct cctgcgcctg ggcatcttgc ggctggccct cctgattctc cccgggctga
      901 ccgccctgac cgccttgtcc cttctgaccc ttctggcctt gtcccttctg acccttctga
      961 ccctggcctt tttgcccttc gcccttctga cccttttggc cttcctggcc gtcttgaggc
     1021 tgaccttcct gcggctggcc tgcttgattc tcaccgggct gcccaccctg acccttctgg
     1081 cccttttgcc cttggccctt ttggccctgc cccttctggc cttcgccctt ttgtccttca
     1141 ccctgctgcg gctggccctt ttggccttgg cccttctgcc cgttctcctg gccgggctgt
     1201 tgctcgccct gttgcggctg gccttcctgc ggctggcgat tgggattgtt gcggttgatc
     1261 tcgtcgttca attcgcgggc cagatcttgt agctcctggc gggcgcggcg gagggcctcg
     1321 gtgtcgtcgc cgagcaccgc ctcggccgcc cggtcgatcc cctcacgcag ctggcgaatg
     1381 ctctggccgg ccggctcttg ctgccggcgg gcgtcgttga ggagcccctg ccgcagcgaa
     1441 cgctcggccg attccagggc ccgctcgggc gtctgctgct ggagattgcg gtcggtgtcg
     1501 tacagccgct cggaaagcag cggctcggtc tcctcggcct gctggatgac ctcgcgcatc
     1561 tgctcctgga tgttgcccag ccgctgccgc tgctgcgaca gctcctcggc gatccgctcg
     1621 cgctcgccgc tatcgcgcag cgtcttgctc ttctcgttcg gccgctcctc gcctgccatt
     1681 cgctgcgaga gttcctgctg cttctcgtcc agctcgcggg ccgcctcgcg catctgccgc
     1741 atctcttcgt tgaagcggcc ggaggcccgg cggcggaact cgttccgcag gtcctcgaac
     1801 tgctgctcgg cccgcgtgcc cgaggccgcg gcccgcgtga cttgctcctc gttgagcgcc
     1861 tcggaggccc ggcggacctg ctcgcgggtt tcctcgagct gctgccgctc ctcggtcatc
     1921 cgctcctggt tttccggggc gtccatccgg ttctgcaact cgtccgtgtc ctgcaggatc
     1981 tgctgctgct cttcctgcag ccgcttgagc tgcttgcgga tttcttcctt ctgcgcctcg
     2041 gtgcgggcct cctccagggc cgactgcagc tccttgacgc gctcgttgag atcgtgctgg
     2101 cggcgggcca gctccttcag gcgattcaaa acctgccggt tctcgcgctc ttcgggcgtt
     2161 tcctgctgct gggcctgggc cgcccgctgc gtctggtagc gattctcgtc gttgcgcagc
     2221 tgcaattggt tcagctgctg ctgttgctgc tgcgagcggg ccccgcgctg ctgctgttgc
     2281 gactgctggc cgcgctgccg ctgctgctgg cggacgacct cgtgctcgcg ggcccgtagc
     2341 tttagcagcg actgataggc ggcctgctcc gcggccatcg ccggcgtcag cggagcggcc
     2401 tgcggacctt cctcggcggc cttgaggtgc tcgaccgctg tagccatcga cttcagcacg
     2461 ctctcgacgt gctcctgcga ctgctcgtcg gtcagccgct cggccagggc tgtcgcctgc
     2521 tcgatcgccg attcctgcga ggtgcggacc tgctcggcgt cggacttcag cgcgtccgtg
     2581 ggcttggatc ccgtctcgcg gcggatcagc ttccaggtcg cgttgatgat ctccttctgc
     2641 agctgggcga gctgctgggc gtcgcgggcg ttctgcccct ggccttgctg ttgctgttgt
     2701 tgctgctgct gctcgccgcc cgcgggctgc tggccctggc ggaaaatttc ctcgaagggt
     2761 cgcacttcgg cgaagtacat gtcgcccatg acgcggcgga tactaccgtc cggtgcgtgg
     2821 tcctcggccc accagtgata agacaaaagc tggtccggct ctgccttcaa gtcctccagc
     2881 ttgatctgct gggccagttc gtgcttctgc ttcgcagcgc cgttttcgct cacgacgaga
     2941 tcacctgcgg ctcgcccgtt gatggaatac gccagacccg cgcgaacgag gccgaaatcg
     3001 tcaaaaacgg tggcccgcac gtccagctct tccagggccg agacttccaa gtcgcgggcg
     3061 gggaacgtcg gcttcaagtt cggcggctga ttcggcagca cgttgatcgt gaagggaatc
     3121 ggcttgggat tcttgcggcc cgcctcatcg accagctcca gcttgagctt tcgcgagcgg
     3181 tcgcacttga tctgggctgt gtagaaatac tgcgtggccc gcgaggaccg ggtcttcgga
     3241 tcggccatgt ccgcgacggg cagcgctggc ggagtggcgg cggccatcgc gatcggctgc
     3301 tgctggtcct cggtaagggt cgcgctcgcc acggccttgt tgaggtagca catcagcgtc
     3361 agctcggtgc cctcgacggc gctgaccgtg cggacgtcct gtaccagctt ctcgggcagc
     3421 tgcgtgtacg ccgggaagac gagcttcgcg tcggctttct ccagccgtgg atattcgaag
     3481 acggtgacct gataggtggg cgtattctgc ccgcccaatt ccacgtggta gctaagcggt
     3541 gcatcgacga gcggaatccg cccgccgaag accgggtcgt cgagactcgg cgacatcggc
     3601 agccgcgact cttcgccggc ctccgtgcga aagacaagcg tcgcctcggc gggcatcgca
     3661 ccggtgatgc gggccagcac gaggaggctc gtgccgcgct cgacctcggt gttgcccggc
     3721 tcgacggtga ccgcgaatga cctggccgag acggagctgg ccgccgcatt ggccaacagc
     3781 gccgcgctct tggccgcagg gccggccgag agggcgatga ccgcgatcac cgccacgaac
     3841 atcgcgaacg tggcgacgtt ggccgcggct gccagggcga tccgcttcat cggcacgact
     3901 tcggtccagg cgttgcggtc ggcgtggtag agggcctgat ggatcacgct cgactgcagg
     3961 tagccgaagc ggccgtccgg caggtcgggc ttctgctcga cggccgccaa gaggcaggtc
     4021 cgcagttcgg ggaaggccgc ttcgatccgc cgcgcgagcc agccgtagtt ccgtgcgctg
     4081 gtggccgcca gccagatgcc ggccacggcg atggccgccc cgaccaggca ggccagaagc
     4141 acgctgacgc tcgggccagc gccgagggcg ccgcgaatcg accacagcgc cagccccgcc
     4201 gccgcggcca cgagccacgc cgccgccagg gcgagccaga agcggagcag ccggtaccgc
     4261 gaggccaccc ggtcgagctc aaaccaaagt cgctgctgat tcatgacgcg ctctcctgtg
     4321 cggctgtggc ggcagcctga atctgccgcg cggcgcggcc ggcccaaaac gtttcgaaca
     4381 cgaggacccc gagcgttccc acgatgatcc agcgccagag cttctgctgg ctttccagtt
     4441 cggtgtcgcg cttctgtcgc atctcgctca gccgctgggc ccgcgtgagc gacgccgaca
     4501 ggcgaacgcc cagctgctgc agctgatcga tctccagcgg tgcggtgttg ctctcgctgg
     4561 cggccaggtt gacggcgaag cgggcctctt ccgcggccag attcgcccga taaatgccgg
     4621 gctggtccgt ttcggaaaag ctcgcggcgt cggcgggcag cttcacgtcg tgcccgtcgg
     4681 gcttgtttac gatcgccgcc atccgccgcg cggcgggcag cggcacgctc tggcccaccg
     4741 ctgcgccggc caattgctcg gtcgtgccgc aggcccggtc gaggatgccg ctgaccagca
     4801 ccacgaactt gttcgagaga gcgagctggc tgtcgtcggg gttccagccg ctggcaaaga
     4861 ccagcgcccg gcccgtgcca atcgtccgct caacgatcgc caattggccg tcgtcgaagc
     4921 gggcgacggc gtgcgagctg gccccttcct tcagcttcag gctccgctgc cgccagaagt
     4981 gaatgcccgt gaagtcgccg taccgcgggc cggcgaacgg cgcgaacagg ggatgcgaga
     5041 agtcgatttc gcccagcagc aggaacttgt cctggttcgt atcgtcctcc gccttggccg
     5101 gcagcagctc
//
LOCUS       DASRJB010000828         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2764830, whole genome shotgun sequence.
ACCESSION   DASRJB010000828 DASRJB010000000
VERSION     DASRJB010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764830"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFB80_21780"
     CDS             complement(<1..835)
                     /locus_tag="VFB80_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN36481.1"
                     /translation="MSTAPPAKPKSLGDILQEFAQHRQLMQQELQKVIVGQHEVIEQL
                     FAAIFTRGHCLLEGVPGLAKTLMVSTLARILDVSFKRIQFTPDLMPSDITGTNVLDED
                     DAGKRNFRFVEGPVFTNILLADEINRTPPKTQAALLQAMQEREVSIGRQTFELPDPFF
                     TIATQNPIEQEGTYPLPEAQLDRFMFNIKVGYPTADEEEKILTQTTRTEKIEVRKILS
                     SVAIVRLQKLVHSVAVSEYIVKYVSRLVRATRPKDETAPQFVKDLVDWGAGPRAGQFL
                     IN"
     gene            1021..3396
                     /locus_tag="VFB80_21785"
     CDS             1021..3396
                     /locus_tag="VFB80_21785"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZN36482.1"
                     /translation="MVRQAAIGCVLIVFLLTQSPAARADITAAQVNKSIADAVEYLES
                     QQQPNGGWTEHGSDPGGGLSALCTLALLNSGRTPEDKSVKNALAYLERLRAPDRIYSV
                     SIMLMAFAQADPKKYALKITSLARWIEAKQVTRDDNYKGGWRYQSPHEQADNSSTQFA
                     ILALHEAERAGVKIPDETWQRAYNYWTQAGMQRPDGGFGYSFGETASGSMTCAGVASL
                     LMCRDRLALGDAQVIDGRIQCCGAQAPNDPVERGLEWLGNRFMIRNNPGNMSNWTLYY
                     LYALERVGRMSGRRLIGGHDWYREGCEYLLANQDRLNCRWTGQGIGEADPLVGTSLAL
                     LFLSKGRRPVVVAKVQHEADALGGVSLDWDHHRRAVQNLTMRVERQWRRDLSWQTISF
                     TGGEAVPVTSVNLLESPVLFLSGSRALSFDDRRKQVLKQYIENGGFIFAEACSGNGCN
                     GAAFDQSFRALMRELFPDSELRRLPPDHAVWYAQEKVDPQHLPKDPEFWLWGLDACCR
                     TSVVYCPRTLSCYWELAHPYRESSFPAQTKAEIEAVTRIGGNVLAYATSRELKEKLDR
                     PQVAVSNPGGKAPRGALVVPKLSHSGGADDAPAALGNLLTVVERQLQMRVDHEKRMVA
                     PTDEKFLDYPLVFMHGRRAFRFSAAERKALKDYLDRGGFLFADAICANQQFADSLRQE
                     LKAIYPDAQFTRIPASHPLFTTEFQGFDLASVTLRDPQIRAQDDPLTTKLVKVAPLLE
                     GLEIDGRIAVILSPYDLSCALEKGASLDCKGYIAPDAARIGVNVLLYALSQ"
     gene            3460..>3886
                     /locus_tag="VFB80_21790"
     CDS             3460..>3886
                     /locus_tag="VFB80_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36483.1"
                     /translation="MPRLLAASLFALSLFVCAARGADEEGFKSIFNGKDLTGWRGDTK
                     FWSVQDGALTAKTTRENPLTYNTFLIWEGGQPGDFELRAKYKIVGGNSGIQYRSKVID
                     EAKFVVGGYQADIDSTPRYSGILYEEKARGVLAERGTKGT"
BASE COUNT          695 a         1254 c         1275 g          662 t
ORIGIN      
        1 cgttgatcag gaactggccg gcgcgcgggc cggcgcccca atcgacgagg tccttcacaa
       61 actgcggggc agtctcgtcc ttggggcgag tggcgcggac cagccgcgag acgtatttca
      121 cgatgtactc gctgaccgcg accgagtgca ccagcttttg cagccgcacg atggccacgg
      181 aggaaagaat cttgcggacc tcgattttct ccgtccgggt cgtctgcgtg aggatcttct
      241 cctcctcgtc ggcggtggga tagccgacct tgatgttgaa catgaagcgg tcgagctggg
      301 cttccggcag cggataagtg ccttcctgct cgatcgggtt ttgcgtggcg atggtgaaga
      361 acggatcggg cagctcgaac gtctgccggc cgatgctgac ctcgcgctcc tgcatcgcct
      421 ggagcagcgc cgcctgcgtc ttgggcggcg tgcggttgat ttcgtccgcc agcaggatgt
      481 tcgtgaagac cggcccctcg acgaaccgga aattccgctt gcccgcgtcg tcctcgtcga
      541 gcacgttggt ccccgtgatg tcgctgggca tcaggtccgg cgtgaactgg atccgcttga
      601 agctcacgtc gaggatgcgg gccagcgtgc tgaccatcag cgtcttggcc agccccggca
      661 cgccctccag caggcagtgc ccgcgggtga agatcgccgc gaagagctgc tcgatcactt
      721 cgtgctggcc gacgattacc ttttgcagct cctgctgcat cagctgacga tgctgggcga
      781 actcctgcag gatgtcgccc agcgatttgg gtttggcggg aggagcggtg gacatgcgga
      841 agagctagtg gctagtgctg gtggctagtg agtaggggta gggctagtgg gaagggctag
      901 gacggcattg ggcaacagca tttgcttcat tcaccagcac tagcactagc caccagcact
      961 cgaccttgcc ctaagataac ggatgggggc agttcgcgaa tgggccaaga gggagcgatc
     1021 atggtgcggc aagcggcgat cggatgcgtg ctgattgtgt tcctgctgac gcagtcgccg
     1081 gccgctcgcg cggacatcac cgcggcccag gtcaacaagt ccattgccga tgcggtcgaa
     1141 tatcttgaaa gccagcagca gcccaatggc ggctggaccg agcacggcag cgatcccggc
     1201 ggcggcctgt cggccctctg cacgctggcc ctgctcaatt cgggccgcac gcccgaagac
     1261 aaaagcgtga agaacgcgct ggcctacctg gagcggctgc gtgctccgga ccggatttac
     1321 tccgtatcga tcatgctgat ggccttcgct caggccgatc caaaaaagta cgccctgaag
     1381 atcacgagcc tggcccgctg gatcgaagcc aagcaagtga cccgcgacga caactacaag
     1441 ggcggctggc gctatcagag cccccacgaa caggccgaca acagtagcac gcagttcgcg
     1501 atcctggcgc tgcacgaggc ggaacgcgcc ggcgtcaaga ttccagacga gacctggcag
     1561 cgggcctaca actactggac acaggcggga atgcagcggc ccgacggcgg ttttggctat
     1621 tccttcggcg agacggccag cggcagcatg acctgcgccg gcgtggcatc gctgctgatg
     1681 tgccgcgacc gcttggccct cggcgacgcc caggtgatcg acggccgcat tcaatgctgc
     1741 ggggcgcagg cgccgaacga cccggtcgag cgcggcctcg aatggctcgg gaaccggttc
     1801 atgatccgca acaaccccgg caacatgtcg aactggacgc tctattacct ctacgccctg
     1861 gaacgcgtcg gccggatgtc gggccgccgg ctgatcggcg gccacgactg gtatcgcgaa
     1921 ggctgcgagt acctgctggc gaaccaggac cggctgaact gccgctggac gggccagggc
     1981 atcggcgagg ccgacccgct cgtcgggacg tcgctggccc tcttgttcct ctccaagggc
     2041 cgccggcccg tcgtcgtcgc caaggttcag cacgaagccg acgccctcgg cggcgtgtcg
     2101 ctcgattggg accaccatcg ccgggccgtg cagaacctca cgatgcgcgt cgagcggcag
     2161 tggcggcgcg acctctcgtg gcagacgatc agcttcaccg gcggcgaggc cgtgccggtc
     2221 accagcgtga acctgctgga gtcacccgtt ttgttcctca gcggctcccg ggcgctgtcg
     2281 ttcgacgacc ggcgaaagca agtgctcaag caatacatcg aaaacggcgg cttcatcttc
     2341 gccgaggcct gcagcggcaa cggctgcaat ggggcggcgt tcgatcagag cttccgcgct
     2401 ttgatgcgcg agctgttccc cgacagcgag ctccgccgcc tcccgccaga ccacgccgtc
     2461 tggtacgccc aggaaaaggt cgatccgcag cacctgccca aggacccgga attctggctg
     2521 tgggggctgg acgcttgctg ccgcaccagc gtcgtctatt gcccgcggac gctgtcgtgc
     2581 tactgggagc tggctcatcc gtaccgcgag tcgagctttc ccgcccagac caaggccgag
     2641 atcgaggcgg tcacgcggat cggcggcaat gtgctggcct atgccaccag ccgcgagctg
     2701 aaggaaaagc tcgaccggcc gcaagtcgcg gtcagtaacc cgggcggcaa ggccccgcgc
     2761 ggggcgctgg tcgtgccgaa gctcagccac agcggcgggg ccgacgacgc gccggccgcc
     2821 ctgggcaacc tgctgaccgt cgtcgagcgg cagctgcaaa tgcgggtcga tcacgagaag
     2881 cggatggtcg cgccgaccga cgagaaattc ctcgattatc cgctggtctt catgcatggc
     2941 cggcgggctt ttcgattctc ggcggccgag cgcaaggccc tgaaggacta tctcgaccgc
     3001 ggcggctttc tgttcgccga cgcgatttgc gcgaaccagc aattcgccga ctcgctgcgg
     3061 caggaactca aggcgatcta tcccgatgcc cagttcacgc gcatcccggc cagccatccg
     3121 ctgttcacga ccgagttcca aggcttcgac ctggccagcg tgacgctccg cgatccgcag
     3181 attcgcgccc aggacgaccc gctgaccacc aagctggtca aggtcgcgcc gctcttggaa
     3241 gggctggaga tcgacgggcg aatcgccgtg atcctgtcgc cgtacgacct gagctgcgca
     3301 ttggaaaagg gcgcttcgct cgactgcaag ggctacatcg cccccgatgc cgcccggatc
     3361 ggcgtcaacg tgctgctcta cgcactgtcg cagtagccgc cgtgggtggc tatgctattc
     3421 ggcatcggcc agcccatcct ctcaccgtag gaacctacca tgccccgact cctcgctgct
     3481 tcgctgttcg ccctctcgct cttcgtttgc gccgcccgcg gcgcggacga ggaaggattc
     3541 aagagcatct tcaacggcaa ggacttgacc ggctggcgag gcgacacgaa gttctggagc
     3601 gtacaggacg gggcgctcac cgcgaaaacg acgcgcgaga atccgctgac ttataacacg
     3661 ttcctgatct gggagggcgg gcagccgggc gatttcgaat tgcgggcgaa gtacaaaatc
     3721 gtgggcggca attcgggcat tcagtaccgc agcaaggtga tcgacgaagc caagtttgtc
     3781 gtcggcggct accaggcgga catcgactcg acgccgcgct acagcggcat cctgtacgaa
     3841 gaaaaggccc gcggcgtcct ggccgagcgg ggcaccaagg gcacgg
//
LOCUS       DASRJB010000829         8843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1096180, whole genome shotgun sequence.
ACCESSION   DASRJB010000829 DASRJB010000000
VERSION     DASRJB010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096180"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..864
                     /locus_tag="VFB80_21795"
     CDS             373..864
                     /locus_tag="VFB80_21795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN36484.1"
                     /translation="MLYLFLFGVAIGIISGLLGIGGGILLVPGLMLLFQFSQQEAQGT
                     SFAVLIPPIGIFAALVYIQNGDVKLPVAGTIAAGYMLGALGGALLVPHVPAYWLRLGF
                     GGLLLYLGLRFVLGAYLPKQAAALPAVLAALLVAIGAWLHGRRAAIATKLPPPDSQTE
                     YHI"
     gene            complement(855..1592)
                     /locus_tag="VFB80_21800"
     CDS             complement(855..1592)
                     /locus_tag="VFB80_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HZN36485.1"
                     /translation="MPSDPAPAKAPARFGLAMRLILLAAIAAAAGLAWYFFRGELRIE
                     ALAAREMELREAVEAHPAAALAIAFAAYVAVTGLSLPGATAMSVLYGWLFGFWRGVVL
                     VSFASTTGATLAFLMSRYLLGNWIQARFGERLAAINAAIEREGALYLLTLRLIPQVPF
                     FVLNAVMGLTRMRVTTYWWVSQLGMLPATIVFVLAGANAPRLKTVAEKGLGSILDWRL
                     MLPLALLAVLPLAIRWAVGRLGGKRQM"
     gene            1699..2769
                     /locus_tag="VFB80_21805"
     CDS             1699..2769
                     /locus_tag="VFB80_21805"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN36486.1"
                     /translation="MSDPKPLENRVKLLRQARGWTQGELAAAAGLSRTGVGAIEAARL
                     VPSVAAAIGLARALSCSVEDLFGPRARGGCEFAWAPAAFPCRYWAAEIAGRTLLFPVE
                     SGPQGGLLHDGVAQHEADLPAQVEQARTTLVLATCDPAAGLLATLYRREGGLRMLVFT
                     RASREALGLVERGIAHAAGIHLAPASAKRGNAALISAAAGPAGMTLLHVARWEEGLAV
                     QPAARLRSASSAARGNFRWVGRSMGAGARRCQDEILGQRRAPRHVAHDHRGVVEAIRS
                     GWADVGVCLRLASEEGQLAFLPVGEEAYDLCFRSDQASDPRLAALVRTVRSAEYRRLL
                     EELPGYRPQAHLGELEDVVPHA"
     gene            2944..3534
                     /locus_tag="VFB80_21810"
     CDS             2944..3534
                     /locus_tag="VFB80_21810"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZN36487.1"
                     /translation="MELGLSICLGLGLSAACGFRVFVPMLGISTAALAGHLHLAYGFE
                     WLGTWPAFACFLTATILEIAAYYVPWLDNLLDSIATPAAVIAGTLATASVITDMSPLL
                     KWTLAIIAGGGIAGVIQSATVLVRGGSTLSTGGATNWLVATAEWIAALCGTVLSMLLP
                     ILAVGLAALVVMIAFSILARFRRPPTRAGQMAGKAA"
     gene            3604..4164
                     /locus_tag="VFB80_21815"
     CDS             3604..4164
                     /locus_tag="VFB80_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36488.1"
                     /translation="MLRFIPTFALAAFVACPLPAQEPPVPGLVGSVILPTGPQAPEQP
                     AAKGPAKVEFFWVEAKPVEGLTQAKGLQTSCDPDELVYVHLKPVLTAKDVAGTRMSNS
                     DLSKNGLPGDHFMINFELTKEAKGKLIAGCVDAGARSLVVVADGTYWGLSYFRKADAA
                     TFSPHAGFISSRATAERIVSACQPVK"
     gene            4178..5437
                     /locus_tag="VFB80_21820"
     CDS             4178..5437
                     /locus_tag="VFB80_21820"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HZN36489.1"
                     /translation="MIRAWERNQLNSQRSGAAIAGLILCIAASAQPASGQPVSNEPAQ
                     WKQSIERPSRSWDYDGKRILGHVGYKVCLWDATTGKLLHKLQWHKEHIRVVQFSPDGK
                     HALSSSWMPPGPMQPELKSKDTRTILWDLTTGGDKAEFPGQVAGEFSPDGKRIVTFSQ
                     RPGVLAAFDAVVWDAFAGRQLAEVKLAEGSDPYWDALHFSPDGDRFAHITNGAFLLYN
                     SSGGVVFDTSDGRELGRVTRDAIRFGGHRYTSNELLATFDRASARLIDLKSGRVVQSI
                     QHDLKTIWGTAWTHDGRRFAAIPSDESEIKISDIETGKMTTGAKNGPYPHRTAIVSPD
                     NSRLVVEWGGANGIEPGLGIYNLNTGTEIARVKLAKWGHLLGFSPDSKTLLVGGAEFV
                     IYNSEDGTEIRTLRLLDDVSFEHDWNH"
     gene            5730..6749
                     /gene="phnD"
                     /locus_tag="VFB80_21825"
     CDS             5730..6749
                     /gene="phnD"
                     /locus_tag="VFB80_21825"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN36490.1"
                     /translation="MMVLLVLLLVVTGACYAYVLYANRHNPDYAFEDIREFVAKAAEH
                     RKLGDAYRDADGDLVADTPSDPGKLQQVDEIGFCLVAGDDPGQTQEEWADFLQALSKA
                     TGKKVSYRAGLENLEAQLAALQGGTLHVAAFSTGEVPVAVNTAGFVPLASPADAEGKY
                     SYEMEILVPAASGSRSPADLKGKRVGFTAMSSNSGAKAPLVILKDQFQMLPGRDYQHY
                     VTGSHQRSIQLLAAGRFDAVCVANDLLASAVAAGDIKTEQYRSIYKSASFPPLCLGVA
                     HDLPPELTAQVKQVFQDFRFEGTSLAKRFGPQGKVRFSPVDYKRDWAYVREINDSLAR
                     LVDTP"
     gene            complement(6791..7624)
                     /locus_tag="VFB80_21830"
     CDS             complement(6791..7624)
                     /locus_tag="VFB80_21830"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN36491.1"
                     /translation="MRSSIAISPHGQFLAFAALAAMLGFGSASAADGVKPLKALLVIG
                     GCCHDYAAQKAILAQGLAARAHVEVTIAYDPDTTTKHLNPVYEKADWAAGFDVVIHDE
                     CSSDVKDKAVIDRILAPHRAGLPAVLLHCGMHSYRSEGYPGVTPWFEFTGLQSTGHGP
                     QAPIDVGFLKTDHAITKGLADWTTINEELYNNSAGKLLESATPLARGRQTTRSKKGES
                     NTSDYVVAWINDYKGTKVFATTLGHNNQTVADARYLDLVTRGLLWSCGKLQPEYLKES
                     K"
     gene            complement(7721..8695)
                     /locus_tag="VFB80_21835"
     CDS             complement(7721..8695)
                     /locus_tag="VFB80_21835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZN36492.1"
                     /translation="MIPATLRMLAGVALAYGVALVLYWPVRRRGGWAWAALACGLAAI
                     LACPLVIPPEHVPLRAVAVFVSVDLVFRLVDFTRQVQSGRRTSVNWPTYARFLIPWPV
                     LLIVFNQRSRQLPPDAPRAPELLRAAVAGGVFAVALSLTLAAQYSPRLRESFLLDHAA
                     KVLLFLVGIEALAQLLLGLERLGGFDSLPFIDRAYQSRTPAEFWFRYNQRVRLWLTLN
                     VFLPAGGRRAPLRAIFAAFLVSALLHELAFGIATSRFDGYQALFFLLQAPAVMLSPAL
                     ERFAAHGPLQSALARGATIAWFSLTSILFFTSADRVLPFFYVGRPWLP"
BASE COUNT         1563 a         2870 c         2931 g         1479 t
ORIGIN      
        1 ccgcaggtcg gagagtggag aaaaacgcag cgtcatcatc gatacctcct cgcaaaaatc
       61 cggttttaag gccgcctgcc aacgggcggc acaatcgata cttcctgttc ccgcgacgac
      121 gtaggcagcc gccggcgaaa gtggaaattg caaccagcgt gccaatcgcc aatcgaggac
      181 ctgcgattgg atcgcaactc gaattcgctt cgagatttgc ggcgatcgcg aactctcgcg
      241 ccgcaaagct cgcgagggcc aaaacaattt ccgccgctgg gcgcctgcca aattgacaat
      301 cgcgagttcc cacggaacgc cgcggactcc acaatgaacg ctcgttcgat ttttttccat
      361 ccggagtccg ccgtgcttta tctctttctg ttcggcgtcg ccattggcat cattagcggc
      421 ctgttgggta tcggcggcgg catcctgctg gtgccgggcc tgatgctgct ctttcagttc
      481 agccagcagg aagctcaggg cacgagcttc gccgtgctga ttccgcccat cggcatcttt
      541 gcggcgctcg tttacatcca gaatggcgac gtcaagctgc cggtcgcggg cacgatcgcc
      601 gcgggctaca tgctgggcgc actcggcggt gcactgctgg tgccgcacgt gcccgcctac
      661 tggctgcggc tgggattcgg cggcctgctg ctgtacttgg ggctgaggtt cgtgctgggc
      721 gcgtacttgc ccaagcaggc cgcggcgctg ccggcggtgc tggccgccct gctggtcgcc
      781 atcggcgcct ggctgcacgg ccggcgggcg gccatcgcca cgaagctgcc gccgcccgac
      841 agccagaccg agtatcacat ctgacgcttg ccgcccaggc ggccaaccgc ccagcggatc
      901 gccaggggca gcacggccag cagggccagc ggcagcatca gccgccagtc gaggatgctg
      961 cccaggcctt tctcggcgac ggtcttcagg cgcggggcat tcgcgcccgc cagcacgaac
     1021 acaatcgtcg ccggcagcat gccgagctgg ctgacccacc agtacgtcgt cacccgcatc
     1081 cgcgtcagcc ccatcaccgc gttgagcacg aagaacggca cctgcggaat cagccgcagc
     1141 gtgagcaggt acagcgcccc ttcccgctcg atggcggcgt tgatcgcggc cagccgctcg
     1201 ccgaagcggg cttgaatcca gttacccagc agatagcggc tcatcaaaaa cgcaagcgtc
     1261 gcccccgtcg tcgaggcgaa gctgacgagc acgactcctc gccagaaccc gaacagccag
     1321 ccgtagagca cgctcattgc cgttgcgccg ggtagcgaca ggccggtcac cgcgacatac
     1381 gcggcgaagg cgatggccag cgccgccgcc gggtgcgcct cgaccgcttc ccgcagctcc
     1441 atttcccgcg cggcgagcgc ctcgatccgc agctctccgc gaaagaaata ccaggccagc
     1501 cccgccgcgg cggcgatcgc ggccagcagg atcagccgca ttgccaggcc gaagcgagcg
     1561 ggggctttcg cgggggcggg atcgctcggc atgcgggcga ttatagttca atcggcactg
     1621 actgcctgcc gccgtcgcgg cgcacagcgg ggggcggatc tcgcaatcaa cacgatagtg
     1681 acataggatg gcagaatcat gtccgatccg aagccactcg aaaaccgcgt gaaattgctc
     1741 cgtcaggccc gcggctggac gcagggcgaa ctggccgccg cggccggact gtcgcggacc
     1801 ggcgtgggcg caatcgaggc ggcgcggctg gtgccgtcgg tggccgcggc gatcggcctg
     1861 gcccgcgccc tgagctgctc ggtcgaggac ttgttcggcc cccgggcgcg cggcggctgc
     1921 gagttcgcct gggctccggc cgcctttccc tgccgctact gggcggcgga aattgccggc
     1981 cgcacgctgc tgtttccggt cgaaagcggc ccgcagggcg gcctcttgca tgacggcgtc
     2041 gcccagcacg aggccgacct gcctgcacag gtcgagcagg cccggaccac gctggtgctg
     2101 gccacgtgcg atccggcggc cgggctcctg gcgaccttgt atcgccgcga aggcggcctg
     2161 cggatgctcg tcttcacgcg ggccagccgc gaggcgctgg gactggtcga gcggggaatc
     2221 gcacacgccg cgggcatcca cctggccccc gccagcgcca agcgcgggaa cgcggcgctg
     2281 atctccgccg cggcgggccc agccggcatg acgctgctgc acgtcgcccg ctgggaggaa
     2341 ggcctggccg tgcagccggc ggcccggctg cggtcggcgt cgagcgcggc ccgcggcaat
     2401 ttccgctggg tcggccgttc aatgggggcc ggcgcgcggc gctgccagga cgaaatcctg
     2461 ggccagcggc gggctccgcg gcacgtggcg cacgatcacc gcggcgtcgt cgaggcgatc
     2521 cgcagcggtt gggccgacgt gggcgtttgc ctccgactgg ccagcgagga agggcagctg
     2581 gcgttcctgc ccgtcggcga ggaagcctat gacttgtgct ttcgcagcga tcaggcgtcc
     2641 gatccgcgcc tggccgcgct ggtccgcacg gtccgctcgg ccgagtaccg ccgcctgctg
     2701 gaagagctgc cgggctaccg gcctcaagcg cacctgggag aactggaaga tgtcgtgccg
     2761 cacgcgtgac gggttggcga tgcccggcag gccgcggccc gccgcctggc ggccttcctc
     2821 cgcgagaggc ccacgcagga ctggatttcc gggttcggca ggggaaaata cgatgatcgg
     2881 cctttgctct ttccggtgaa ggtggaatag gcaagattcg ccgctctcgt gcgaatcgct
     2941 ggtatggaac ttgggctctc gatctgtctg ggtctgggct tgtcggccgc gtgcggcttt
     3001 cgcgtcttcg tgcccatgct gggcatcagc acggcggctt tggccggcca tctgcacctg
     3061 gcttacggct tcgagtggct gggcacgtgg ccggcgtttg cctgtttttt gacggcgaca
     3121 atcctggaga tcgcggccta ctacgtgccc tggctcgaca acctgctcga ttccatcgcc
     3181 acgcccgcgg cggtcattgc gggcacgctc gccacggcct cggtcatcac cgacatgtcg
     3241 ccgctgctca agtggacgct ggcgatcatc gccggcggcg gcatcgcggg cgtcattcaa
     3301 tcggcgaccg tgctggtccg cggcggctcg acgctgtcca cgggcggcgc gaccaactgg
     3361 ctggtcgcta ccgccgagtg gattgccgcg ctctgcggca ccgtgctctc catgctgctg
     3421 ccgatcctgg ccgtcgggct ggccgcgctg gtggtgatga tcgcattcag catcctggct
     3481 cgctttcgcc gcccacccac ccgcgccggg caaatggcgg gaaaggctgc gtagtcggcc
     3541 tccatctctg gtaggttgtt gccagcacct cgaaggttaa ctgtcctcaa tcccacgcct
     3601 ggcatgctcc gcttcatccc caccttcgcg ctggctgcct tcgtcgcttg cccgttgccg
     3661 gcccaagagc cgccggtgcc gggcctggtt ggcagcgtga ttctccccac gggcccgcag
     3721 gcgcccgagc agcctgcggc caaagggccg gccaaggtcg agttcttctg ggtcgaagcg
     3781 aagccagtgg aagggctgac gcaggccaag gggctgcaga cgagctgcga tccggacgag
     3841 ctggtgtacg tccacctgaa gccggtgctc accgccaagg acgtggccgg cacgaggatg
     3901 tccaacagcg acctctccaa gaacggtctt ccgggcgacc acttcatgat caacttcgag
     3961 ctgaccaagg aagccaaggg caagctgatc gccggctgcg tcgacgctgg cgcccgctca
     4021 ctcgtcgtcg tcgccgacgg cacctactgg ggcctcagct acttccgcaa agccgacgcc
     4081 gccaccttct ccccccacgc cggctttatc tcctcccgcg ccaccgccga acggatcgtc
     4141 agcgcctgcc agccggtgaa gtgagtgcga tggtcttatg attcgtgcat gggaaagaaa
     4201 ccagctgaat tcgcagagat ctggcgcggc catagcggga ctcattttgt gcatcgctgc
     4261 ttctgcacaa ccggcgtctg gccagccggt gtcaaatgaa ccggcccaat ggaagcagag
     4321 cattgaacgg ccgagcagga gttgggacta cgacggaaag cggattcttg ggcacgtcgg
     4381 ctacaaagtc tgcttatggg acgcaactac cgggaagctg cttcataaac tgcaatggca
     4441 caaagagcat atccgagtcg tccaattcag ccccgatggc aagcacgccc tcagcagctc
     4501 gtggatgcct cctggaccga tgcagcctga gctgaaatcg aaagacactc gcacgattct
     4561 ttgggacctg acgaccggtg gggacaaagc tgagtttccg ggacaggtcg ccggcgaatt
     4621 cagtcccgac ggcaagcgaa tcgtcacatt cagccagcgc ccaggcgttt tggctgcgtt
     4681 cgatgccgtc gtctgggacg catttgcagg gcgtcaactg gctgaagtaa aactcgccga
     4741 gggcagcgat ccctattggg atgccttgca tttttcgccc gatggggatc gattcgccca
     4801 tattacgaat ggcgcctttc tcctctacaa ctcgagcgga ggagtagtct tcgacacgag
     4861 cgacggacgc gagctaggaa gggtcacaag ggacgcgata agattcggtg ggcaccgtta
     4921 cacttcgaac gaattactgg caactttcga ccgtgcgagt gccagactaa ttgacctcaa
     4981 atcggggcga gtcgtccaaa gcattcaaca cgacctgaag accatttggg gcacggcatg
     5041 gacccacgac ggtcgccgat tcgccgcgat tccctccgat gaaagtgaga tcaagatttc
     5101 ggacatcgag acgggaaaga tgacgaccgg agcaaagaac ggcccctacc ctcatcgaac
     5161 cgccatcgtc agtccggaca atagccgact ggtggtcgaa tggggcggtg caaatggtat
     5221 tgagccaggg ctgggcatct acaatctgaa cacgggcaca gaaatcgcca gggtcaagct
     5281 cgccaagtgg ggacacctgc tcggcttttc gccagacagc aagaccttgc tcgtcggcgg
     5341 tgcagagttc gtgatctaca actcagagga cggcacagaa atccgcacgc tcaggctatt
     5401 ggacgacgtc agctttgaac acgactggaa tcactaggct acatttcggc agagcacaat
     5461 cggcgttggg ctgtggctga agctggtcct cgttgccgct ttccactcgt atctttaccg
     5521 agcactccga ccgggctatc ttgccgaggt tgctggaagt ggtcggcctg ctctattgcc
     5581 gacaggaggg catgcaactg ccgcgagctg ctctcgcacc ctggcggcgc gtgcggcggc
     5641 aacgaactcc ttgcgcttac cccggtgttc tcatgacgac tccgcagcca gcgccgccga
     5701 ctccccgacc agcgagccgc gcgcgccgca tgatggtact gctcgtattg ctcctcgtgg
     5761 tcacgggggc ttgttacgcg tatgtgctgt acgccaaccg gcacaatccg gactacgcct
     5821 tcgaggacat ccgtgaattc gtggccaagg cagcggagca tcggaaacta ggcgacgcct
     5881 atcgcgacgc cgacggcgac ctggtagcgg acactccgag cgatccgggc aagcttcagc
     5941 aagtggacga gatcggtttt tgtctcgtgg cgggcgacga cccgggccaa acgcaggagg
     6001 agtgggcgga cttcctccag gcactgtcca aagcgacggg gaagaaggtg agctaccgcg
     6061 ctggcctgga aaatctggaa gcccaactgg ccgccctgca gggcggcacg cttcacgtcg
     6121 cggctttcag caccggcgaa gtgccggtgg cggtcaacac ggctggcttc gtgccactgg
     6181 cctcgccggc cgacgcggag ggcaagtact cctacgagat ggagatcctc gtgccggccg
     6241 cgagcggcag ccgttcgccg gccgatctca aaggcaagcg ggtcgggttt acggccatgt
     6301 cgtccaactc cggggcgaag gcgccgctgg tgatcctcaa ggaccagttt cagatgctgc
     6361 ctggccgcga ctatcagcac tacgtgactg ggagccacca gcgctcgatc cagttgctgg
     6421 ctgccggcag gttcgacgcg gtgtgcgtgg ccaacgacct cttggccagc gctgtggccg
     6481 cgggcgacat caagaccgag cagtatcggt cgatctacaa atcggcgtca ttcccaccgc
     6541 tctgcctggg cgtagcacac gatctccccc cggagctcac cgcccaggtg aaacaggtct
     6601 ttcaggattt ccggttcgag gggacctcgc tcgcgaagcg gtttggcccg cagggcaagg
     6661 tgcggttctc cccggttgac tacaagcggg actgggcgta cgtccgcgag atcaacgact
     6721 ccctggcgcg gctggtcgat accccgtagg tagcggcacg acggcacacg gagtgtgact
     6781 gctactgtgg ctacttcgat tctttgagat actccggctg cagcttgccg caggaccaga
     6841 ggaggccgcg ggtgacgaga tcgaggtagc gagcgtcggc gacggtctga ttgttgtggc
     6901 cgagcgtggt ggcaaagacc ttggtgccct tgtagtcgtt gatccacgcg acgacgtagt
     6961 cgctggtgtt ggactcaccc tttttgctgc gggtggtctg gcggccgcgg gccagcggcg
     7021 tggccgattc gagcagcttg ccggcgctgt tgttgtacag ttcttcgttg atcgtggtcc
     7081 aatcggccag gcccttggtg atcgcgtggt cggtcttcaa aaagccgacg tcgatcggcg
     7141 cctgcgggcc gtggccggtg ctctgcaaac cggtgaactc gaaccagggc gtcacgccgg
     7201 gataaccttc gctgcggtag gagtgcatgc cgcagtgcag caggacggcg ggcaatcccg
     7261 cccggtgcgg cgccaggatg cggtcgatga ccgccttgtc cttgacgtcg gacgagcact
     7321 cgtcgtgaat cacgacgtca aagccggccg cccagtcggc cttttcgtaa acggggttga
     7381 ggtgcttggt ggtcgtgtcc ggatcgtagg cgatcgtcac ttcgacgtgg gcccgcgcgg
     7441 caaggccttg ggcgaggatc gccttctgag ccgcgtagtc gtggcagcag ccgccgatca
     7501 cgagcagcgc cttgagcggc ttaacgccgt cggcggcgct cgcgctgccg aaaccgagca
     7561 tggcggcgag cgccgcgaaa gccaggaact gcccgtgcgg gctgatggcg atggaactcc
     7621 gcatgtcgtc cctcccagga aaaccgctcg cccagtggcg tgtgattgtc ccagtttgga
     7681 tctcggcggc ggcgattgca acgaggcggg accaggaccc tcaaggcagc cacggccggc
     7741 cgacgtagaa aaatggcagc acgcggtcgg cgctggtgaa gaagagaatc gacgtgagcg
     7801 agaaccaggc gatcgtcgct ccgcgggcca gggcggactg cagcgggccg tgggcggcaa
     7861 accgctccaa cgcgggcgag agcatcacgg ctggagcctg caggaggaag aacagggcct
     7921 ggtaaccatc aaaacgcgag gtggcaatgc cgaacgccag ctcgtgaagc aaggcgctga
     7981 ccaggaacgc cgcgaaaatc gcgcgcagcg gggcccgccg cccgccggcg ggcagaaaca
     8041 cgttgagcgt cagccacagc cgcacgcgct ggttgtagcg gaaccagaac tcggcgggcg
     8101 tccgcgactg gtacgcccga tcgatgaacg gcagcgaatc aaagccgccc agccgctcca
     8161 agccgagcag cagctgcgcg agcgcttcga tgccgaccag gaagagcagc actttcgcag
     8221 catggtcgag gagaaacgac tcgcgtaatc gcgggctgta ctgagcggcc agagtcagcg
     8281 acagggcgac cgcgaacacg ccgccggcga ctgctgcccg cagcaattcc ggcgcgcgcg
     8341 gtgcgtccgg cggcagctgg cggctgcgct ggttgaacac gatcaagagc accggccagg
     8401 gaatcaggaa gcgggcgtag gtgggccaat tgaccgaagt tctgcggccg ctctggacct
     8461 gccgggtgaa atcgaccagg cgaaacacga gatcgacgga cacaaacacc gccacagccc
     8521 gcagcggaac atgctccggt ggaatgacca gcgggcacgc caggatcgcc gccagaccgc
     8581 aggccagcgc cgcccaagcc catcccccgc gccggcgaac tggccaataa agcaccaggg
     8641 ccacgccgta cgccagcgcg acacccgcca gcatcctgag ggtagccggg atcatcgctg
     8701 gccactatac gccgacaaga gttcgggcgc aggatttgcc ggttctagcg ccctgacggc
     8761 acacggcgtg tgcctgctac cggagggccc gcgcggcaag gcccttggcg aggatctcct
     8821 tctgagccgc attgtcgtgg cag
//
LOCUS       DASRJB010000830         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1933971, whole genome shotgun sequence.
ACCESSION   DASRJB010000830 DASRJB010000000
VERSION     DASRJB010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933971"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VFB80_21840"
     CDS             complement(<1..1549)
                     /locus_tag="VFB80_21840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN36493.1"
                     /translation="MIDSGEGTIRDQQPVLPSGGPGEPAPAGDDEEVVRVLDQCLCDL
                     ERGIPLDTERLLADHPAIAHRLRACLSGLSLLDEATSPKAPWAASAPPEIADHTIVRE
                     IGRGGMGIVYEAMQRSQDRRVALKVLPFAAALDPRQRTRFQNEAHAASRLRHPHIVPV
                     YETGSAGGVHFFTMKLIEGVSLATVLAEQRKEGGVWPATAPAGLRSSLLFVRRSSDSE
                     YIGEVARLAQQAAEGLDHAHEVGVIHRDIKPGNLLVDERGQLWITDFGLASIQGGEGL
                     TGTGDFLGTLRYMSPEQASARRGVVDHRTDVYALGVTLYELLTLTPAFSGRDRQEVLT
                     QLALDDPRPPRRLNPAIPIDLETIVLKAMAKAPEERYATARALADDLGRFRAGQPIAA
                     RRPGLAALAARWVRRRRGLAAAILLFIVATTATLAVSTGLVWMALQSEQRQRALADAK
                     ELESRRHRYAAQMTSAMQEWRDGNVARVLALLEQHRPAPGAEDMRCFEWFHLWHLCQH
                     AQRGVLRG"
     gene            complement(1574..2167)
                     /locus_tag="VFB80_21845"
     CDS             complement(1574..2167)
                     /locus_tag="VFB80_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36494.1"
                     /translation="MAGVQTPELLVEQARAGDSEALGQLLEQYRHYLRLLAQSQVGRN
                     LRVRLDPSDLVQETLLEAQRDFQKFAGTGEAELTAWLRRILVRNLADQLKHHHSQKRD
                     YQREQPLEVLVEQAHAALAAPLSTPSAHLARREQAVVLAQALAQLPPDYREVITLRHL
                     EGNSFDAIADKMGRTSGAVRMLWMRALERLGGLMEAQ"
     gene            complement(2286..>2723)
                     /locus_tag="VFB80_21850"
     CDS             complement(2286..>2723)
                     /locus_tag="VFB80_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36495.1"
                     /translation="FLKEVLTNGLSDSVFYFYSVKSSLKWWKLFREVDEANPGDWTYE
                     PARYRPRLDPRVATQLVRHRQRLPIAGELLGSGELEIDAYPSPLGKYGQVLEFVHDPD
                     DLWYVAASFEEFLDQSIACMEQSIAKDPEHARGLLEDPRTKDW"
BASE COUNT          441 a          917 c          907 g          458 t
ORIGIN      
        1 cgccgcggag cactccccgc tgggcatgct gacagagatg ccagagatga aaccactcga
       61 agcagcgcat gtcttccgca cccggagctg ggcgatgctg ctccaagagc gccagcacgc
      121 gggcgacgtt gccgtcgcgc cactcctgca tggcggaggt catctgggcg gcgtagcggt
      181 ggcggcggct ctccagctct ttggcgtcgg ccagggcccg ctgccgctgc tcgctttgca
      241 gggccatcca gaccaggccc gtgctgacgg cgagggtcgc ggtggtcgcc acaatgaaca
      301 gcaggatcgc ggcggccaga ccgcggcggc ggcggaccca gcgggcggcc agcgccgcga
      361 gccccgggcg gcgggccgcg atcggctggc ccgcgcggaa ccggcccagg tcgtcggcca
      421 gcgcccgcgc cgtggcatag cgctcctccg gcgctttggc cattgccttc agaacgatcg
      481 tttcgaggtc gatcggaatc gccggattca gacgccgcgg cggccgcgga tcgtcgagcg
      541 ccagttgcgt caggacttcc tgccggtcgc ggccgctgaa ggccggcgtg agcgtcagca
      601 gctcgtagag cgtgacgccc agggcataca cgtcggtgcg gtgatcgacg acgccccgcc
      661 gtgcggaggc ctgctccgga ctcatgtagc gcagcgttcc cagaaagtca ccggttccgg
      721 tcagcccttc gcccccctga atcgacgcca gaccgaaatc ggtgatccag agctggccgc
      781 gctcgtccac cagcagattg ccgggtttga tgtcgcggtg aatcacgccg acttcgtgcg
      841 cgtgatcgag cccttcggcg gcctgctggg ccaagcgcgc gacttcgccg atgtattccg
      901 agtcgctgga gcgccggacg aaaagcagcg aggaccgcaa gcccgccgga gctgtggcgg
      961 gccagacgcc gccttccttt cgctgctcgg ccaacacggt cgccaggctc actccttcaa
     1021 ttagcttcat ggtgaagaag tgcacgccgc cggccgaacc ggtttcgtag accggcacga
     1081 tgtgcggatg gcgcagccgc gaggcggcgt gggcctcgtt ctgaaagcgg gtccgctgcc
     1141 gcgggtcgag agccgccgcg aacgggagca ccttgagcgc gacgcggcgg tcctgcgagc
     1201 gctgcatggc ctcatagacg atgcccatcc cgccgcggcc gatctcgcgg acgatggtgt
     1261 ggtcggcgat ttccggcggg gcgctcgctg cccatggcgc tttgggggag gtcgcctcat
     1321 cgagcagcga caacccagac aggcaggccc gcagacggtg ggcgatcgcc ggatgatcgg
     1381 ccagcaggcg ctcggtatcg aggggaatgc cgcgctccag atcgcacagg cactggtcca
     1441 gcacgcggac gacttcctcg tcgtccccgg cgggcgcggg ctcgccgggc ccgccggacg
     1501 gcaggaccgg ctgctggtcg cgaatcgtcc cttcgccgga gtcgatcact gagcctccat
     1561 cactgggcct ccatcactgg gcctccatca aacctcccag ccgctccagc gcccgcatcc
     1621 agagcatccg cacagcgccg gacgtgcggc ccatcttgtc ggcgatcgca tcgaagctgt
     1681 tcccctccag gtgccgcagc gtaatcacct cgcgataatc gggcggcagc tgggccagcg
     1741 cctgggccag cacgaccgcc tgctcgcggc gggccaggtg ggcgctgggc gtggagagcg
     1801 gggcggccag cgccgcatgg gcctgctcga ccagcacctc caggggctgc tcgcgctggt
     1861 agtcgcgctt ctgggagtgg tggtgcttga gctggtcggc cagattccgc accaggatcc
     1921 gccgcagcca ggcggtcagc tccgcctcgc cggtgccggc gaatttctgg aagtcccgct
     1981 gagcctccag caacgtttcc tggaccaggt ccgacgggtc cagccgcacg cgcagattcc
     2041 ggccgacctg cgactgggcc agcagccgca ggtagtggcg gtactgctcc aggagctggc
     2101 cgagggcctc ggaatcgccg gctcgggcct gttccaccag cagctccggc gtctgcacac
     2161 cggccacgtt cggctcactt ctgggcgagg agggcggcga aatgtcacgc tctagcggga
     2221 gtcgatttta gttcgatttc ttggtcccac caacacatcg gcgcgcactt gtcgtgtcag
     2281 agagcctacc aatctttcgt gcgggggtct tctaacaaac cgcgtgcgtg ctccggatct
     2341 ttcgcgatcg actgctccat gcaagcgatt gattggtcga ggaattcttc gaaagatgcg
     2401 gcgacatacc agaggtcatc aggatcgtga acgaattcca gaacctgacc atatttgccg
     2461 agcgggcttg gataggcatc gatctcgagt tcgccgcttc ctaacaactc gccagcaatt
     2521 ggcagccttt gccgatggcg taccaattga gtggctaccc gcggatccag cctgggtcga
     2581 taacgagctg gctcgtacgt ccagtcgccg gggttggctt cgtccacttc cctgaacagc
     2641 ttccaccact taagtgaact cttgactgag taaaagtaga acaccgagtc gctcaaaccg
     2701 ttcgtcagta cttccttcag aaa
//
LOCUS       DASRJB010000831         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_243113, whole genome shotgun sequence.
ACCESSION   DASRJB010000831 DASRJB010000000
VERSION     DASRJB010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243113"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..464)
                     /locus_tag="VFB80_21855"
     CDS             complement(45..464)
                     /locus_tag="VFB80_21855"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN36496.1"
                     /translation="MHGELSDVCCEQNADQARQRRAIEKLLKAGADIHETDKNGVTPL
                     HHAVRFRSPAAVRCLLEHGANANQACKRSGGTPLHRAVTTTGAPSTAGKRDEVRQIIE
                     LLLRFGADPKLKNKSGKTPADYVRDEAIRKLLKPRRQ"
     gene            complement(488..1105)
                     /locus_tag="VFB80_21860"
     CDS             complement(488..1105)
                     /locus_tag="VFB80_21860"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36497.1"
                     /translation="MTRFLLCLLAAACIAALPADLPLAAAEPPGQTQLFGVQAEGSSF
                     LYVFDRSLSMKGQPLAAAKRELVASLGRLKRLQQFQILFYNQNARLMQPQQMQFADES
                     GLRAAESYIAGVQASGATDHVQALQLALRMRPDVIFFLTDADEPQLSAAELESIQRKN
                     LGSIIHVIEFKEGQTQGAGTTLRKLAEQNRGEYKFVDVSKLSAGP"
     gene            complement(1119..>2155)
                     /locus_tag="VFB80_21865"
     CDS             complement(1119..>2155)
                     /locus_tag="VFB80_21865"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36498.1"
                     /translation="SEGNMRYLATIMTLALPFAVPMTILAQAEGGKTLWAGMIGLDTS
                     HVPAFAKIFNSEAAAGDVAGIKIVVGYPGGTDIPASRDRVARFTEQIRGMGIEIVDTI
                     PKLLEKVDVVLIESVDGRIHLQEAIPAIQAGKPLWIDKPVAGSLSDAIVIYELAKKHR
                     VPVFSSSSLRFGPGIQAVLKDESIGPVLGAATWGSCSYSPGTPDLFFYGIHGVEPLFT
                     IMGTGCETVSRVQTDGTDFVTGVWKGGRVGTYRGVRRGAATAGATVFGTKKIAPADKL
                     GGYEDLCREIGKFFKTGQPPVAAEETIEIFAFMEAADESKRRGGAAVSLADVLSKARA
                     EAAKRLAQLD"
BASE COUNT          342 a          719 c          709 g          385 t
ORIGIN      
        1 caaccggcgc aaatcgcagg cccacaagca ggtatttcat acgtctattg ccgccgcggc
       61 ttcaacagtt tccggatcgc ctcgtcccga acgtaatccg ccggcgtctt gccgctcttg
      121 ttcttgagct tgggatcggc cccgaagcgg agcagcagct cgataatctg ccggacctcg
      181 tcgcgcttgc cggcggtgga gggggcaccg gtggtggtga cggcgcggtg cagcggagtg
      241 ccgccggagc gcttgcaggc ctgatttgca ttggccccgt gctccagcag gcaccgcacg
      301 gcggcggggc tgcggaagcg gaccgcgtgg tgcagcggcg tcacgccgtt cttgtcggtc
      361 tcgtgaatgt ccgccccggc cttgagcagc ttctcgatcg cccgccgctg cctggcctgg
      421 tccgcgttct gctcgcagca gacgtccgac agctcgccgt gcatggttca ccgtgcagtg
      481 gcgcttgtta cgggccggcg gaaagcttcg aaacgtccac gaacttgtat tcgccgcggt
      541 tctgctcggc cagcttgcgg agcgtcgtcc cggcgccttg cgtctggccc tccttgaact
      601 caatcacgtg gatgatcgag ccgaggtttt tgcgctgaat cgactccaat tcggcggcgc
      661 tcagctgcgg ctcgtcggcg tcggtcagaa agaaaatcac atccggccgc atccgcaggg
      721 ccagctgcaa ggcctggacg tggtcggtcg cccccgaggc ttgaacgccg gcgatgtacg
      781 actcggccgc gcggaggccg ctctcgtcgg cgaactgcat ctgctgcggc tgcatcaggc
      841 gggcgttctg attgtagaac aggatctgaa actgctgcag ccgcttcagc cggcccaggc
      901 tggccaccag ctcgcgcttg gcggccgcca gcggctgccc tttcatgctg agcgagcggt
      961 caaacacgta gaggaagctg ctaccctcgg cctgcacgcc aaagagctgc gtctggccgg
     1021 gcggctcagc ggcagcgagc ggcagatcgg ctggcagggc cgcgatgcac gcggcggcga
     1081 gcaggcacaa gaggaagcga gtcatggccg gttccgatct agtcgagctg cgccagtcgc
     1141 ttcgcggctt cggcgcgggc cttggaaagc acgtcggcca gcgaaaccgc cgcgccgccg
     1201 cggcgcttgc tttcgtcggc cgcctccatg aaggcaaaga tctcgatcgt ctcctccgcg
     1261 gcgactggcg gctggccggt cttgaagaat ttgccaattt cgcggcagag gtcctcgtaa
     1321 cctcccagct tgtcggccgg cgcgatcttc ttcgtgccaa agaccgttgc gccggccgtg
     1381 gctgccccgc ggcgaacgcc ccgataggtc cccacgcggc cgcctttcca cacgcccgtg
     1441 acgaagtccg tgccgtcggt ttgcacgcgg ctgaccgttt cgcagccggt gcccatgatc
     1501 gtgaacagcg gctcgacgcc gtgaatcccg tagaagaaca ggtcgggcgt tccgggcgaa
     1561 taggagcacg agccccaggt ggccgccccg aggaccgggc cgatcgattc atccttcagc
     1621 acggcctgta tccccggccc gaagcggagc gacgagctgg aaaaaaccgg cacgcggtgc
     1681 ttcttcgcca actcgtagat cacgatggcg tcgctcagcg atccggcgac cggcttgtcg
     1741 atccacagcg gcttgcccgc ctggatggcc ggaatcgcct cctggaggtg aatccgccca
     1801 tcgacgctct cgatgagcac gacatcgacc ttttccagca gcttggggat cgtatccacg
     1861 atctcgatgc ccatgccgcg aatctgctcg gtgaagcggg ccacgcggtc gcggctggcg
     1921 ggaatgtcgg tgccgccggg atagcccacg acgatcttga tccccgccac gtcgcccgcg
     1981 gcggcctcgc tgttgaaaat cttggcgaac gccggcacgt gcgaagtatc gagcccgatc
     2041 atgccggccc agagggtctt gccgccctcg gcctgcgcca agatggtcat cggaacggcg
     2101 aacggaagtg cgagcgtcat gatggtggcc aggtatcgca tgttaccctc cgagt
//
LOCUS       DASRJB010000832        11764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3621749, whole genome shotgun sequence.
ACCESSION   DASRJB010000832 DASRJB010000000
VERSION     DASRJB010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621749"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFB80_21870"
     CDS             complement(<1..262)
                     /locus_tag="VFB80_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36499.1"
                     /translation="MHKSILVFTAAVMCVPLSGCGGCGSPSAAQMRKYAQRRSAPEVE
                     PPKTAAPSEQSKSGGSVKAGSPPMPPPAAAAPAVAATVEVKPE"
     gene            552..2702
                     /gene="pilM"
                     /locus_tag="VFB80_21875"
     CDS             552..2702
                     /gene="pilM"
                     /locus_tag="VFB80_21875"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZN36500.1"
                     /translation="MEATPLAASAAALITCRHCQRPNPPARRFCGGCGQSLWERCPQC
                     DAECQSDEQFCGSCGTDIRAQLGQRSSEHQAALDEALALAGQHEYDAAVTRLQSIAAI
                     GDPRLAPIAQRAREEIERLKRAEREQSAIAAQALSRADELIAAQAYDQAQTLLEQIPP
                     PLRSKEAQRLLDRAAACQSEAISLSSEIRAAVESKRYGDLLPKLDKLLALKPNHAQAR
                     QLAGQLVGSLIKSAAARLSQHRYQEALDQLDRIPAVVSTPEAGQLSAQARELNCLLLE
                     TQNAALADEQLVSIASRLVELAPQNAAAARLRAQAVERAKALPAELRLRAPNWAPVPE
                     RTLLGLPVDWLAHPTRAAASGDAAKSLREHPGQFFVAFGLALQALDLAAVGVDLMPRE
                     KGSLLSKLPALNLGGKRAPEAAWGLDLSDTALKALKLVREAKDGSLKIEAAEFLLHSQ
                     PLQHPAAEANRCAIVAQTLAEFAGRAGDLKGVKLCTNIAGGRVLGRFFELPPMPARKV
                     AESVQYEARHQLPVALDELSWSYAIQDDVAGKAADEQPRRIVVQATRDAHVRDRLASF
                     KAAGLAIDAVQSDCLALHSALVHELELSGEGAVAAIDVGVAGTNVVVSSPSCAWFRTL
                     AHGGDHYSRELAKALGCTAEEAELQKRQPARVCGYHLLCEAWQPLFVQLGSEIERSLA
                     TYRKLYPEHPVRQLYGLGGAFRAQGLLRYLRSGK"
     gene            2793..3518
                     /locus_tag="VFB80_21880"
     CDS             2793..3518
                     /locus_tag="VFB80_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36501.1"
                     /translation="MKLVSLSASLLVLALGLSAHAADNTPPEGFAALFNGKDLTGWKG
                     IPLRVNDKGRASPLSMPQRLSGPKDELEKAQKLGDESAREHWKAENGVLVFDGKGQNL
                     CTAKDYGNFELYVDWKIEPKGDSGIYLRGSPQVQIWDPKDEAQTKNGADKGSGSLWNN
                     KKNPRFPNVLADNPIGEWNTFHIKMVGEKVTIHLNGKLVVDNVTMENLWEPEKPIYAK
                     GPIELQNHGNTLYFKNIYVKELP"
     gene            3766..7440
                     /locus_tag="VFB80_21885"
     CDS             3766..7440
                     /locus_tag="VFB80_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36502.1"
                     /translation="MSQFSRWVASGCLVLVAALALAQEPPKPADAPMPADAEKPAKEQ
                     TIYIPYTKLRQIFEKEGRGVFVPYEEFQRLWKAAAAAAKKIEDIKPPVAALISEIDSE
                     ATVSRDVMTVNAKLQIEVLTEGWHYVHLRLSDAAIRSATIGDKPARIIFEAGKGHWVL
                     LEAKRPAADGGQSPPYKPEKYELKLEYSKAFTKQPGTNSVEFDAPQAPVNRWQIKIPE
                     PGVKVNVQPNISTTDAGAEKMGDADPAAAAPDAKKETLVQAFVGAAPQVRIDWTAKAE
                     GAAGLAALVTVQARQDITIDEGVVRTRAHLTYDIQRADVTVLTVEVPADHNVVNVFDP
                     NVQKWEKKTAGQTQTLTITLFQPTRGTQNVTIELEKFAGDKEMPQEMMHAEIKAPVVR
                     AVQAGTADALSNIGRQQGVVVVRLGSALRGEATSRTGLLQIDAAELPAPLAGQEWNFA
                     YRYAALPFDLNLSVEKLLPQIEVEELVEVYLEPNQTTANLLAVLNIQRAGIFQIELDV
                     PEGFEVRTVQGRDVAGAVAVAVDSHHLEDVEYVPNPAQPDVKAKRKTKLLVNLSRRAL
                     GKVALWAELVKRQDDPNLLTPTNVLSVINVPLPRVQPSSVARTSGRVLVYAPESLRIN
                     PKESKGLRPISPAEAVQVVASTRGGRFPQLRELLAYAYTQEAASLIAEGQRRKPYIEA
                     RELLTAHVESGVVRYEATFFFDIKYSSVKTLRLDVPAAIAGDLRNQTPAIREQRIDPQ
                     PPDVAAGYVAWQLVGEGELLGSVTAKFTWEKKLGELPVGTAVAIDLPAFKAVGVDRFW
                     GQIAASKAETIEITVKEGYKGLRPIDPQRDLLTSVPNAARAFEFHDDWSLAMQATRFQ
                     LEEVKRTSIERAVVRAVVTRGDQVAVQALYRLRSARQRIPVKLPGVDPANTSGVLDST
                     PLRINNAPAPLEHDGTYFYIPLTGHAADEPVLVELRYTVTGDQSSLELPDFSGDPLTE
                     ADDPAVQQVHLVAHLPEEQKLLGIRGPWTDESASLWRQIAPKDDQALLADVRQGVECG
                     NAGDDFPVDGRRFVFSTLRPEPAAEGAVHMTTLHRNTVNFGVFLLVAIVGLVLTPQPA
                     GTRLWYLAGLFVTVVLIAVFAPTLAQAILEPVDWGQPPSDGPQQGLSPLSWAVLLVLV
                     VWGVRFLAWFIPGVIAWCTDSFKKAAAATAVAAAAAAATSAAATPPPAPEGAAGQSPF
                     SPATPAPPAGEERREGGSNG"
     gene            7562..>11764
                     /locus_tag="VFB80_21890"
     CDS             7562..>11764
                     /locus_tag="VFB80_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36503.1"
                     /translation="MPFEDLNVILDNDKHRAFLTREEYEELVKLAKAKPQKPAPTKVA
                     LIAAEYDGQLEDGRALITGQLQIEVLEDGLFALPLDLGGVGIRSALLDEKPAPLSRNE
                     QQQPIVLVQGKGLHKLELKLTAPLQTAAAQQTLQVTLPSVGATKLKLAVPGNVDVKGG
                     AAVYSRSYDMAANRTQLELLPQRGGMSVVMSLNNRLLQDQRVVVARSVIVDEVTQGYE
                     RIHATISYRVLHGAVEKLRLAVPAGFEVTKVETLQLARWEEKLEGGRSILEATLREAT
                     SEQIVLHLTANRSPAAGGDWLAGLANWKFPRLEPLETAGSVAVVGLLVEDRLKPEQIA
                     SSNLVPIDSAALTGAIPASVLKAEPGAPAVRQVVSYYAPASEYSLTAKFARPPAGLKV
                     AANSLLVIGDQGLTLQGGFALTPQAESLFQLAFTLPAGWQVTQVTAEGGAVLPIERYP
                     AAAGGTRVSVRLPKGIPAGQTQTVNYQATITPAGWLTEWTTQKVDFPKVLLEGATSES
                     GAVAAQTTDDLLVRPDQLASLTPLLDNEKAAYGLGNIATALAYRFENRDFAASLVVER
                     TAPSLAAQVYSILRLERDNLVAHYELSYDVREARTRRVAFSLPSSTPAELTIRGIGST
                     VVKEFTSTTEGQRRRWSVQLAERQIGEVKLAVEFTQRLPETEVQNQALPLVQAEDVEY
                     QSAVVAVEGDAELDVSVKTTAARPVDIGELAGSEYPLQNLRRVIGTFGYVGTQTQVAA
                     TIARREAYTLPPALIERAELVTKVSTSGVAQSVARYDLVTKATLLEIRLPLRSTLWTV
                     FLDNEPTKPQREGESLLLSLPAQQRPSVRKLQVVYETPSQRLGLSGRVEAVSPTLLVR
                     ATGTDAEREVPQADLLWQLILPSGYIVRRAGGSVFTDIPEREMPVKVVAAWAYQLAGG
                     YRPWYWSPVMSSPLSDDTSFSMGWADATPRAGTGAQHGGELYGTPAPTEGAPPPPAMT
                     PPMAAESRPNEEPGRDTRKAADTAGASREVRDMAKSMPAPRTPVTQAESAPTVPQFDA
                     AQPGQQAAAAVQGRIGKEFWALEGVGSLWINIEANEPATTFRSLGEQPQLEAVVTDQR
                     RLTAAAWGLALLVLLVGVGLTQRQARTKVSYVIFVLLSATVPLLLTEVFDEVALVFDY
                     VFYAGCALVPYYLAAAVALAAYRHVKSRVPYCCPPVTQIVALLIAAGLALAAVPASAQ
                     TAAEDMTPVAVPADAIIVPYDTDKAREVDPKQKLLVPYAKYVELWNLAHPDKKIEIAP
                     PPVGFAIAGSAYEATLGTGDFLALVGRLQIDVFSDKPVSVPLHLGGGVLEKATVDGQA
                     AKLQLVEPEGQPPAQAAKPNSGGGIPPRMLLLHLTGKGRKSVELHVRLGLSRQGGWRI
                     VRGQLPVGPAAALTLTAPTAGTE"
BASE COUNT         2066 a         3889 c         4057 g         1752 t
ORIGIN      
        1 cctcgggctt cacttccaca gtcgccgcta ctgcgggcgc agcagcggcc ggcggcggca
       61 tcggcgggct gccggctttc accgacccac ccgacttgga ctgttccgaa ggcgcggctg
      121 tctttggcgg ttcgacctcc ggcgcgctgc ggcgctgggc atacttccgc atctgggcgg
      181 ccgatggact cccacagccg ccgcaacctg acagcggcac gcacatcacc gccgcggtga
      241 aaaccagaat tgacttgtgc atatcgccag ctgccagtgg gccgcccggt gaattcagcg
      301 aacttagcgc cggggcgtga agcaggcgtt aatttggcgc taaggcttgg gtggtggaac
      361 attaggacga acggtggcac gccaaccccg gcacgccaac cccggcacgc caaccccgac
      421 gcgccagcga gggatttgta aagcgatgcc atgctttgtt cttcacctcg tcttcaccgc
      481 tgcttaatgg ctatcccgga tgctggctga ccggcattac ttcatcaggt cgcggtcagc
      541 aggaaatcgg aatggaggcg actcccctgg ccgcttcggc ggctgctctc atcacttgcc
      601 gccactgcca gcggccaaac ccgcccgcgc ggcggttttg cgggggttgc ggacagtcgc
      661 tgtgggaacg ctgcccacag tgcgacgccg agtgccaatc ggacgagcaa ttctgcggct
      721 cctgcggaac tgacatccgt gcccagctag gccagcggag cagcgagcac caggccgcgc
      781 tcgacgaagc gctggccctg gcggggcagc acgaatacga cgcggccgtc acgcgactgc
      841 aatccatcgc cgcgatcggc gatccgcggc tagcgccaat cgcgcagcgg gcccgcgaag
      901 agatcgagcg gctgaaacgg gccgagcgcg agcaatcggc gatcgcggcc caggcgctat
      961 cccgggcgga cgagctgatc gcggcccagg cctacgacca ggcccagacg cttctcgaac
     1021 aaatcccgcc gccgctgcgc tcgaaggagg ctcagcggct cctggaccgc gcagccgcgt
     1081 gccagagcga ggcgatctcg ctgagcagcg aaatccgcgc ggcggtcgag agcaagcgtt
     1141 atggcgattt gcttcccaag ctcgacaaac tgctcgcgct caagcccaac cacgcccagg
     1201 cccggcaact ggcggggcaa ctcgtgggca gcctcatcaa gagcgccgcc gcccgtttat
     1261 cgcagcatcg atatcaggaa gcgctcgacc agctggaccg gattcccgcg gtcgtcagca
     1321 ctccggaggc cgggcagcta agcgcgcagg ccagggagct gaactgcctg ctgctggaaa
     1381 ctcagaacgc ggcgctggcc gatgagcagc tcgtgtcgat tgccagccgg ctggtagagc
     1441 tggctcccca aaacgccgcg gcggcgcgat tgcgggcaca ggccgtcgag cgagccaagg
     1501 cgctgccggc cgagctgcgg ttgcgagccc cgaattgggc gccggttcca gagcggacgc
     1561 tgctgggcct gcccgtcgat tggctggccc atccgacgcg cgctgcggcc agcggcgatg
     1621 cggcgaaatc gttgcgcgag catcccgggc agtttttcgt cgccttcggg ctcgccctcc
     1681 aggcgctgga cctggcagcc gtcggcgtcg atctcatgcc gcgcgagaaa gggagcctgc
     1741 tcagcaagtt gccggcgctg aacctgggag gcaagcgcgc gcccgaggcc gcctggggct
     1801 tggacctgag cgataccgcg ctaaaggcct tgaagctcgt ccgcgaggcc aaagacggct
     1861 cgctaaaaat cgaagccgcc gaatttctgc tgcattcgca gccgctacag catcccgcgg
     1921 ccgaggccaa ccgctgcgcg atcgtggccc agactctcgc cgagtttgcc ggccgcgcgg
     1981 gcgatctcaa gggggtgaag ctctgcacca acatcgccgg cggtcgcgtg ctgggtcggt
     2041 tcttcgagct gccgccgatg cccgcccgca aggtcgccga gtcggtccag tacgaggccc
     2101 ggcaccagct gccggtcgcg ctcgacgagc tctcgtggtc gtatgcgatc caggacgatg
     2161 tcgcaggcaa ggctgccgac gagcagccgc ggcggatcgt cgtgcaggcg acgcgcgacg
     2221 cgcacgtgcg ggaccgcctg gcgagcttca aggccgcggg actggcgatc gatgccgttc
     2281 aaagcgactg cctcgcgctg cacagcgcgc tggtgcacga gctggagctg tctggcgagg
     2341 gcgcggtcgc ggcgatcgac gtcggtgtgg cgggcacgaa cgtcgtcgtc agctcgccga
     2401 gctgcgcctg gttccgcacg ctggcccacg gcggcgacca ctactcccgc gagctggcca
     2461 aggcgctagg gtgcaccgcc gaggaggcgg agctgcaaaa acgccagcct gcccgcgtct
     2521 gcggctacca cctgctgtgc gaggcctggc agccgctctt cgtccagctc ggcagcgaga
     2581 tcgaacgttc gctggcgacc tatcgcaagc tctatcccga gcatccggtc cgccagctct
     2641 acggcctggg gggcgcgttc cgcgcacagg ggctgctgcg gtacctgcgg tcggggaagt
     2701 agcgggcgag tgcagcggcg gtggcgattg gctatgatgg tcgcacccat tggtcaagcc
     2761 tccgcccaca tcgatatttc gaggagcccg ccatgaaact cgtcagcctc tccgcgtcac
     2821 ttctcgtcct ggccttgggc ctgagcgccc acgccgctga caatacgccc ccggaaggtt
     2881 ttgcggcgct gttcaacgga aaggatctga ccggctggaa gggcatcccc ctgcgggtca
     2941 acgacaaagg acgcgcgtcc cccctctcga tgccgcagag gctcagtggc ccgaaggacg
     3001 agctggaaaa ggcgcagaag ctgggcgacg aatcggcccg ggaacattgg aaggccgaaa
     3061 acggcgtgct cgtgtttgac ggcaagggcc agaacctctg caccgcgaag gactatggca
     3121 actttgagct gtacgtcgat tggaagatcg agccgaaggg ggacagcggc atctacctcc
     3181 gcggctcgcc gcaggtgcag atttgggatc cgaaggacga ggcccagacc aagaacggcg
     3241 ccgacaaggg ctcgggctcg ctctggaaca acaagaagaa tccccgcttc cccaacgtgc
     3301 tggccgacaa tcccatcggc gagtggaaca cgttccacat caagatggtc ggcgagaaag
     3361 tgacgatcca cctcaacggc aagctggtcg tcgataacgt gacgatggag aacctctggg
     3421 agcccgagaa gccgatctac gccaaggggc cgatcgagct gcagaaccac ggcaacacgc
     3481 tttatttcaa gaacatctac gtcaaagagc tgccgtagtc ctgctttgta gtgcggcggc
     3541 gaaatctaca catcgaagtg cggttatgcg gtctggtggg cgcgtgccgg cgcggattcc
     3601 ctgcccaaaa ccacgcagcc aaaacctaga tttacaaagc cgttacagcc ggtaacggcg
     3661 tttggaatct caaagtctag gtaaatcgtc taagttgaca gtctgagggt ccgcgctcgc
     3721 tcgatagcgc ggccggcggt tcttgattgg ggaaggatgc gtcttatgtc gcaattctcg
     3781 cggtgggtgg cgtcgggatg tttggtgctc gtagcagcct tggctctggc tcaagagccg
     3841 ccgaagccgg ccgatgcgcc catgccggcg gacgccgaga agccggccaa agagcagacg
     3901 atctacatcc cctacaccaa gctgcggcag atatttgaga aggaaggccg cggcgtgttc
     3961 gtgccgtacg aggagtttca gcggctgtgg aaggcggcgg ctgcggcggc aaagaagatc
     4021 gaagacatca agccgccggt ggccgcgctg atttccgaga tcgacagcga ggcgaccgtc
     4081 agccgcgacg tgatgacggt caatgccaag ctgcaaatcg aagtgctcac ggagggttgg
     4141 cactacgtcc acctgaggct gagcgacgcc gcgatccgtt cggcgacgat cggcgacaag
     4201 cccgcgcgga tcatcttcga ggcgggcaag ggtcactggg tgctgctgga ggcgaagcgg
     4261 ccggcggccg atggcgggca gagcccaccc tacaagccgg agaagtacga gctgaagctg
     4321 gaatacagca aggcgttcac gaagcagccg gggacgaaca gcgtggagtt cgacgcgccg
     4381 caggctccgg tgaatcgctg gcagatcaag attcccgagc ccggcgtaaa ggtgaacgtg
     4441 cagccgaaca tttcgacgac cgacgccggg gcggaaaaga tgggtgacgc cgacccggcg
     4501 gctgccgcgc cggacgccaa gaaggaaaca ctggtgcagg cgttcgtcgg ggccgcgccg
     4561 caggtccgta tcgactggac cgccaaggcc gaaggagccg cagggttggc ggccctggtc
     4621 acggtgcagg cccggcagga catcacgatc gacgaggggg tcgtgcgcac gcgggcccac
     4681 ctgacctacg acatccagcg ggccgacgtg accgtgctga cggtcgaggt gccggcggac
     4741 cacaacgtcg tcaacgtctt cgaccccaac gtgcagaagt gggagaagaa gaccgccggg
     4801 cagacgcaga ctctcacgat cacgctcttt cagccgaccc gcggcaccca gaacgtcacg
     4861 atcgagctgg agaaattcgc cggcgacaag gaaatgccgc aggagatgat gcacgccgag
     4921 atcaaggccc cggtcgtgcg ggcggtgcag gccggcacgg ccgacgcact gagcaacatc
     4981 ggccggcagc aaggcgtcgt cgtcgtgcgg ctgggctcgg ccctgcgggg cgaggcgacc
     5041 tcgcggacgg gcttgctaca gatcgacgcg gccgaattgc ccgcgccgct ggccggccag
     5101 gaatggaact ttgcctaccg ctacgcagcc ctgccgttcg acctgaacct cagcgtcgag
     5161 aagctgctgc cgcagattga ggtcgaggag ctggtcgagg tgtacctgga gccgaaccag
     5221 acgacggcca acttgctggc cgtattgaac atccaacggg ccggcatttt ccaaatcgag
     5281 ctggacgtgc ccgaaggatt cgaagtgcgg accgttcagg ggcgcgatgt cgccggggcc
     5341 gtagccgtcg cggtcgattc gcaccacctg gaagacgtcg aatacgtgcc gaatcccgcg
     5401 cagccggacg tgaaggccaa gcggaagacc aagctgctgg tgaacctgtc gcggcgggcg
     5461 ctcggcaagg tcgcgctgtg ggccgaactg gtcaagcggc aggacgatcc gaatttgctc
     5521 acgccgacga acgtgctgtc ggtcatcaac gtgccgctgc cccgcgtgca gccgtcgagc
     5581 gtcgcccgca ccagcggccg cgtcttggtg tatgcccccg agagcctgcg gatcaatccg
     5641 aaggagtcca aggggctgcg gccgatctcg ccggccgagg cggtgcaggt cgtggcatcg
     5701 acgcgcggcg ggcgattccc gcagctccgc gagcttttgg cctacgccta cacgcaggag
     5761 gccgcgagcc tgatcgccga ggggcagcgc cgcaaaccgt acatcgaggc ccgcgagctg
     5821 ctcacggcgc acgtcgaatc gggtgtcgtg cggtatgaag cgacattctt cttcgacatc
     5881 aagtacagca gcgtgaagac gctgcggctg gacgtgccgg cggcgatcgc cggcgatttg
     5941 cggaatcaga cgccggcgat tcgcgagcag cggatcgacc cgcagccgcc ggatgtggcc
     6001 gcgggctacg tggcctggca gctagtcggt gaaggggagc tgctggggag cgtgacggcc
     6061 aagttcacct gggagaagaa gctgggcgag ctgccggtcg gcacggcggt cgccatcgac
     6121 ctgccggcgt tcaaggcggt gggcgtcgat cggttctggg ggcaaatcgc tgccagcaag
     6181 gccgagacga tcgagatcac ggtgaaggaa ggctacaagg gcctgcggcc gatcgacccg
     6241 cagcgcgacc tcttgaccag cgtgcccaac gccgcgcggg cgttcgaatt ccacgacgac
     6301 tggtcgctcg ccatgcaggc cacgcggttc cagctggaag aggtcaagcg gacgagcatc
     6361 gagcgggcgg tcgtccgcgc ggtcgtcacc cgcggcgatc aggtcgcggt gcaggcgctc
     6421 taccggctgc ggtcggcgcg gcagcggatt ccggtgaagc tgccgggcgt cgatccggcg
     6481 aatacgtcgg gcgtgctcga ttcgacgccg ctgcggatca acaacgctcc cgcgccgctg
     6541 gagcacgacg ggacgtattt ctacatcccg ctcaccggcc acgccgccga cgagccggtg
     6601 ctggtcgagt tgcggtacac ggtcaccggc gatcaatcct cgctggagct gcccgacttt
     6661 tcgggcgatc cgctgaccga ggccgacgac ccggccgttc agcaggtgca cctggtggct
     6721 catttgcctg aggagcaaaa gctgctgggc attcgcggcc cgtggacgga tgaatcggcg
     6781 tcgctgtggc ggcaaattgc ccccaaggac gatcaggcgc tattggccga cgtccgccag
     6841 ggcgtggagt gcggcaatgc gggggacgat tttcccgtgg atggccggcg gtttgtcttc
     6901 tcgacgctgc gacccgagcc ggcggccgaa ggggccgtgc acatgacgac actccaccgc
     6961 aacaccgtga atttcggcgt cttcctgctg gttgcgatcg tggggctcgt cctgacgccg
     7021 cagccggcgg gcacgcggct gtggtacctg gcgggactgt tcgtgacggt ggtgttgatc
     7081 gccgtgttcg ccccgacgct ggcccaggcg attctcgagc cggtcgattg gggccaacca
     7141 ccgtcggacg ggccccagca gggcctgtcg ccgctgtcgt gggcggtgct gctggtgctg
     7201 gtcgtctggg gcgtgcggtt cctggcctgg ttcattccgg gcgtcattgc ctggtgcacc
     7261 gattcgttca agaaggcggc ggccgcgacg gcggttgcgg ccgcagcggc agcggctacc
     7321 agcgctgccg ccacaccgcc tcctgcgcca gaaggcgctg ccgggcagag tccattctcg
     7381 cccgccacgc cagcgccgcc cgccggcgag gagcggcggg aaggagggag caatggatag
     7441 cactccctac ctccgagccg caggctggtt tgttgccata gtcgcactgt ccacgtcctc
     7501 gttgcgtgcc cagccagcga agcccgtcga ggctgacccg ccgccaattc gcgaaatcta
     7561 cgtgcccttc gaggatctca acgtcatcct cgacaacgac aagcaccggg cgtttctgac
     7621 gcgcgaggaa tacgaagagc tggtgaagct ggccaaggcc aagccgcaga agccagcgcc
     7681 gaccaaggtg gcgctcatcg cggcggaata cgacgggcag ctcgaagacg gccgggcgct
     7741 gatcactggc caactgcaaa tcgaagtgct cgaggacggg ctattcgccc tgccgctcga
     7801 tctgggcggc gtcggcatcc gctcggcgct gctcgacgag aagcccgctc ccctgtcgcg
     7861 gaacgagcag cagcagccga tcgtgctggt gcagggcaag ggcctgcaca agctggagtt
     7921 gaagctcacc gcgccgctgc aaacggccgc ggcccagcag accctgcagg tcacgctgcc
     7981 gtcggtcggc gcgacgaagc tcaagctggc cgtgccgggc aacgtcgatg tgaagggcgg
     8041 tgcggcggtc tacagccgct cgtacgacat ggccgcgaac cgcacgcagc tggaattgct
     8101 gccgcagcgg ggcgggatgt cggtggtgat gtcgctcaac aatcgcctct tgcaggacca
     8161 gcgggtcgtc gtggcccgca gcgtcatcgt cgatgaagtc acgcaaggct acgagcggat
     8221 tcacgccacg atctcctacc gcgtgctgca cggggcggtc gagaaactgc ggctggccgt
     8281 gccggccgga ttcgaagtga ccaaggtcga gaccctgcag ctcgcccgct gggaggagaa
     8341 gctcgaaggc gggcggtcga ttctcgaagc cacgctccgc gaggcgacca gcgagcagat
     8401 cgtgctgcat ctgacggcca accgctcgcc ggccgctggc ggcgactggc tggccggcct
     8461 ggccaactgg aaattcccgc gcttggagcc gctcgaaacg gcgggcagtg tcgcagtcgt
     8521 gggcctgctc gtcgaggacc gcctgaaacc ggagcagatc gcctccagca acctggtgcc
     8581 gatcgattcg gcggccctca ccggcgcgat tcccgccagc gtgctcaagg ccgaaccggg
     8641 cgcgccggcc gtgcggcagg tcgtgagcta ctatgcgccg gccagcgaat acagcctgac
     8701 cgcgaagttc gcgcggccgc cggccgggct gaaagtcgcc gccaattcgc tgctggtgat
     8761 cggcgaccag gggcttacgc tgcagggcgg cttcgccctc acgccgcagg ccgagagcct
     8821 gttccagctc gccttcacgc tgcccgccgg ctggcaagtg acgcaggtca ccgcggaggg
     8881 cggcgccgtg ctgccgatcg agcgctatcc ggccgcagcc ggcggcacgc gggtcagcgt
     8941 ccgcctgccc aagggcatcc cggccggcca gacacagacc gtgaactacc aggcaaccat
     9001 tacgcccgcg ggctggctga ccgagtggac cacgcagaaa gtcgatttcc ccaaggtgct
     9061 gctggaaggt gcgacgagcg aaagcggcgc ggtcgccgcc cagaccaccg acgacctgct
     9121 cgtgaggccc gatcagctgg cgagcctgac gccgctcttg gacaacgaga aggcggccta
     9181 tggcctgggg aatattgcca cggcgctcgc ctaccgcttc gagaaccgcg acttcgccgc
     9241 cagcctcgtc gtggagcgga ccgcgccgtc gctggcggcc caggtctatt cgatcctccg
     9301 cctggagcgc gacaacctcg tagcccacta cgagctgagc tacgacgttc gcgaagcccg
     9361 cacgcggcgg gtcgcgttct cgctacccag cagcacgccc gccgagctca ccatccgcgg
     9421 catcggcagc acggtagtga aagaattcac cagcacgacc gagggccagc ggaggcgctg
     9481 gagcgtgcag ctcgccgagc ggcaaatcgg cgaggtcaag ctggcggtcg agttcacgca
     9541 gcggctgccc gagacggagg tgcagaacca ggcgctgccg ctggtgcagg ccgaagacgt
     9601 cgaataccag tcggccgtgg tggcggtcga aggagacgcc gagctggacg tgagcgtcaa
     9661 gacgacggcg gcccgcccgg tcgatatcgg cgagttggcc ggctcggagt atccgctgca
     9721 aaacctgcgg cgcgtgatcg gcacgttcgg ctacgtcggc acgcagacgc aggttgccgc
     9781 gacgatcgcc cgccgcgaag cctataccct gccgccggcc ctgatcgagc gggcggagct
     9841 ggtgaccaag gtttccacga gcggcgtggc ccagtcggtc gcccgctacg acctggtgac
     9901 gaaggccacg ctcttggaaa tccgcttgcc gctgcgctcg accctgtgga ccgtctttct
     9961 cgataacgag ccgaccaagc cgcagcgcga gggggaaagc ctgctgctca gcctgccggc
    10021 ccagcagcgc cccagcgtcc gcaaactgca agtggtctat gaaacgccga gccagcggct
    10081 gggcctgtcg ggccgcgtcg aggccgtcag cccgacgctc ctggtgcggg cgaccggcac
    10141 cgacgccgag cgcgaagtgc cgcaggccga cctgctgtgg cagctgatcc tgcccagcgg
    10201 ctacatcgtc cgccgcgccg gcggcagcgt cttcaccgac attcccgagc gggaaatgcc
    10261 ggtcaaagtc gtcgccgctt gggcgtatca attggccggc ggttatcggc cctggtactg
    10321 gtcgccggtg atgagcagcc ctttgagcga cgacaccagc tttagcatgg ggtgggcaga
    10381 cgcaacccca cgtgcaggca ccggggcgca gcacggcggc gaattgtatg ggactccagc
    10441 tccgaccgaa ggcgctccgc cgccgccggc catgacgccg ccgatggcgg ccgaatcgcg
    10501 gcccaacgaa gagcccgggc gcgacacgag gaaggccgcc gacaccgcag gagcgtcccg
    10561 cgaggtgcgc gatatggcga agtccatgcc ggctccccgc acgccggtta cccaggcgga
    10621 atcggccccg accgtgcccc aattcgacgc cgcgcagccc ggccagcaag cggccgccgc
    10681 cgtacagggc cggatcggca aggaattctg ggccctggag ggcgtcggtt cgctctggat
    10741 caacatcgaa gccaacgagc cggccacgac gttccgcagc ctgggcgagc agccgcagct
    10801 ggaggcggtg gtgaccgacc agcggcggct gacggcggcc gcttggggcc tggccctgct
    10861 ggtgctgctg gtcggcgtgg gcctgactca gcgccaggcg cggaccaagg tgagctacgt
    10921 gatcttcgtg ctgctcagcg cgaccgtgcc gctgctgctc acggaagtgt tcgacgaagt
    10981 ggcgctggta ttcgattacg tcttctacgc cggctgcgcg cttgttccgt attatctcgc
    11041 cgcagcggtc gcgctcgcgg cttaccggca cgtgaagagc cgggtgcctt attgctgccc
    11101 gccggtgacg cagatcgtcg cgctgctgat cgcagccggg ctcgcgctgg ccgccgtccc
    11161 ggccagcgct cagaccgcgg ccgaagacat gacgccggtc gccgtccccg ccgacgcgat
    11221 catcgtcccc tacgacaccg acaaggcgcg cgaggtcgat ccgaagcaga agctgctcgt
    11281 gccgtatgcc aaatacgtcg agctgtggaa cctggcgcat cccgacaaga agatcgagat
    11341 cgcgccgccg ccggtcggct ttgccatcgc cggctcggcg tacgaagcga cgctgggcac
    11401 cggcgacttc ctcgcgctgg tcggccgcct gcaaatcgac gtcttcagcg acaagccggt
    11461 cagcgtgccg ctgcacctgg gcggcggcgt gctggagaaa gcgaccgtcg atggccaggc
    11521 cgcgaagctg caactcgtcg agccggaggg ccaaccgccg gcccaggccg ccaagccgaa
    11581 ttcgggtgga ggcatccctc cccgcatgct cctgctccac ctgaccggca aaggccgcaa
    11641 gagcgtcgag ctgcacgtgc ggctgggcct ttcgcggcag ggcggctggc gaatcgtgcg
    11701 cgggcaactg cccgtcggcc cggccgccgc gctgacgctg acagccccca cggccggcac
    11761 cgaa
//
LOCUS       DASRJB010000833         5304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_243725, whole genome shotgun sequence.
ACCESSION   DASRJB010000833 DASRJB010000000
VERSION     DASRJB010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243725"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFB80_21895"
     CDS             <1..265
                     /locus_tag="VFB80_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36504.1"
                     /translation="RPIAVAHLVHCPTLVTVAANDQLIPPKMIDRLVARLPNSALAKY
                     PGDHFQIYHGEAFENLAAAQADFLARQLLSEDSSPALAYGRAA"
     gene            complement(285..530)
                     /locus_tag="VFB80_21900"
     CDS             complement(285..530)
                     /locus_tag="VFB80_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36505.1"
                     /translation="MTIDQLRQMHQARPFRPFDIHLADGRSLPVEHPEFLSQSAGGRT
                     LAVAVPDTDAIEVVDLLLVTSLKQRPDGTPRRGRYGR"
     gene            complement(627..1319)
                     /locus_tag="VFB80_21905"
     CDS             complement(627..1319)
                     /locus_tag="VFB80_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36506.1"
                     /translation="MGFLKKFFRGVFHEGAQAAPAHSSFEHLTEEQLEAHLGVQRYDR
                     FTLTDAIRPSYDLKVVPKQGYRHDFYLDEESKTKVPVLMAAATREILFDVFLELIEPL
                     GTEVDVVLETSHNRESAGHTDLYREHIDLPVFKSLLWDYEDLLLNDGCTGLAVLNPSI
                     PQEVQFDEHKLLIVYGQPLRDYESVFHRFGIRCDQKLKFITEAEHVHSSSDKYAQRFE
                     ELQMTLGMDCGY"
     gene            1770..3347
                     /locus_tag="VFB80_21910"
     CDS             1770..3347
                     /locus_tag="VFB80_21910"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN36507.1"
                     /translation="MQRLPRYYGQLAAAIGLALAVAACEHSPPAAPQPQVQAAAAQPA
                     AFPPPPPDAADGIPGAVETWDSLAMRAGEQFVRVGYSRTTVVKTAGPAGQAVIRTCNF
                     TRTILERAGQSVQQDLKVVSLDTPDGRLVAFETQMGEIATTGLVQGDQLQISTTSAGR
                     TQKTSIPWQAEWGGLFAAEQSVRRQPLTTGERRTVRGLVPMLNVPGDTVLAAGDDETV
                     DLPGGPRKLLKVQATMQIGSQQIESTQWADERGETLKAVVPAVGQETLRTTKDDALRR
                     TAGGKLDLLVNSTVPLAGGFPNAQRTKKAVYLANVKNGKIDGVFSNSLSQHVAIKDDR
                     TAEVTVIAVRPNDPKVAGRVPSPTADDQSPSSYIQSDDPAIIRLANSVANGETDSWTI
                     ACALEKHVDSTIKLKNFSQAFSTAAEVARTLEGDCTEHAVLFAALCRARNIPARCAFG
                     LIYFEPLKGFAFHMWNEVWIGDRWVPMDPTLGQGGIAADHLQLGSTSLSGQAALADLL
                     GVMTAFGRLELQLVAAE"
     gene            3453..4106
                     /locus_tag="VFB80_21915"
     CDS             3453..4106
                     /locus_tag="VFB80_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN36508.1"
                     /translation="MSVTPIVAKLSPDQAILRTERMTLRPLTQGDAPIVQQLASEKDV
                     ASTTRLIPHPYPPGMAATWIAALPELYQRAEMIVWGVAMDEGPLLGTVRLTLYPVDNH
                     AEMGYWIGKPFWNNGYCTEAARAVVSYGFDVLGLERIYANYLARNPASGRVLHKLGMQ
                     QEGLLRRHRRKFGRYEDLIVCGLLKSEYQKLKRRPASPPRTDVQPALQPEASVPPAG"
     gene            4120..4923
                     /locus_tag="VFB80_21920"
     CDS             4120..4923
                     /locus_tag="VFB80_21920"
                     /inference="COORDINATES: protein motif:HMM:NF025602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN36509.1"
                     /translation="MEPVLLIRRELMSNYEYQFASRHFPVVESRVLCGERLVIGRYSV
                     LPLYAELDRDVKLLGSRLINSFEQHHWVAAFEYYRDLKEFTPETWDDDTIYQCRHQGP
                     FVVKGKTKSRKWQWKTHMFAATKSEALQLGQRLKDDAEIGEQGVVYRRYIPLRTFGLG
                     KDGLPFTNEWRFFYFGEQRLSYAYYWPGSDYLQKASIPAAAVALADRIAAITARFVQF
                     FTLDMAETEAGEWILIEVNDGQASVPSEHDLDELYGNLRQAVAANAAQA"
     gene            complement(4924..>5304)
                     /locus_tag="VFB80_21925"
     CDS             complement(4924..>5304)
                     /locus_tag="VFB80_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36510.1"
                     /translation="NEIMTGWIGPGTSLPISRITVASLADLGYSVNIAAADPFAPPGS
                     VSSSFASTTGTSGGTAGVLSPEEISSAVDQALAAINTFRQGVRSFVDEVTQRPRQTPL
                     EDSVDALLVDAATLRESLREIFLV"
BASE COUNT          991 a         1748 c         1671 g          894 t
ORIGIN      
        1 ccggccgatt gccgtggcgc acctggttca ttgccccacg ctcgtcacgg ttgcggcgaa
       61 cgaccagctc atcccgccca agatgatcga caggctcgtc gccaggctgc ccaattccgc
      121 gctggcgaaa tacccaggag accactttca gatctaccac ggcgaggcgt tcgaaaacct
      181 cgcggcggcg caggcggatt tcctggcacg ccagttgttg tcggaagatt catcgcccgc
      241 gctggcctac ggacgtgccg catgaggggg cgggcctggc caaactaccg tccgtatcgt
      301 cctcgccgag gcgtgccgtc cggccgctgc ttcaagctgg tcacgaggag caaatcgacg
      361 acctcgatag catcggtgtc cgggacagcg acggccaggg tccttccgcc agccgattgc
      421 gagagaaact ccgggtgctc aactggaagc gaccggccat cggccaaatg aatgtcaaac
      481 ggccgaaagg gccgggcctg atgcatctgg cgcagctggt cgatggtcat gacgagattc
      541 tatacgcagc cccgagccgg ttcaattttg ccgtgtggcc gcgtagagcc gctacggaat
      601 cgtcgccgaa gcaacggctt tagccgttag tacccgcaat ccattcccag cgtcatctgc
      661 agctcttcga accgctgggc gtacttgtcg ctcgacgaat gcacgtgctc ggcttcggtg
      721 atgaacttca gcttctggtc gcagcggatg ccgaagcggt ggaagacgct ctcgtaatcc
      781 cgcagcggct gcccataaac gatcagcagc ttgtgctcgt cgaactgcac ttcctgcggg
      841 atcgacggat tgagcaccgc caggcccgtg cagccgtcgt tgagcagcag gtcttcatag
      901 tcccagagca ggcttttgaa gaccggcagg tcgatgtgct cgcgatacag gtcggtgtgg
      961 ccggccgact cgcggttgtg gctggtctcg aggacgacat cgacctccgt gcccagcggc
     1021 tcgatcagct ccaggaacac gtcgaacaga atctcgcggg tggccgcggc catcaggacg
     1081 ggaaccttgg tcttcgattc ctcgtcgaga tagaagtcgt ggcggtagcc ctgcttgggc
     1141 acgaccttga ggtcgtacga cgggcggatc gcatcggtta gcgtgaaccg gtcgtaccgc
     1201 tgcacgccca ggtgggcttc gagctgctct tccgtcaggt gctcgaagct gctgtgggcc
     1261 ggagcagctt gcgccccttc atggaatacg cctcggaaaa acttcttcag gaagcccatc
     1321 ggaaacctcg ggtcttgcgg ggggcggcga agtctcggct gttcccggcg gtttggtggc
     1381 cgccggccaa tcgttggaaa tctgcgcaag cgtaggtcaa agtttcgccc ggttcccgcg
     1441 gctcgccgcc gtctggcgac ccaggccgta cggtcggccg cataaccggc actttcggca
     1501 aagccgcgac gcaggccgcg gttgggactg gtgcgctgcg gatggcggcg cgaaccgccg
     1561 cccccgccgc gagcggcgcg aaacgatccc ggcaaatcta accctcgaac tcgcagcggc
     1621 cacttcggcg cgtggcttgc cagccaaatt cagcccgcag tcagccgctc gtggcgcgga
     1681 gcgaaaccct gcgggcgctg gcgcggcaca aaggctgggt cgcgatataa tcttggaaac
     1741 ttccacgact cgactaggga cgcctgttga tgcaacggct gccacgctat tacggacaac
     1801 ttgccgcggc gatcggattg gccttggccg tcgccgcctg cgagcattcc ccgcccgccg
     1861 ccccgcagcc gcaggttcaa gcggccgcgg cgcagccggc ggcatttccg ccaccgccgc
     1921 ccgatgcggc cgatggaatt cccggcgccg tggaaacgtg ggattcgctg gcgatgcggg
     1981 cgggggagca gttcgtccgc gtcggctact cgcggaccac ggtggtcaaa accgccggac
     2041 cagcgggaca agcggtcatc cgcacgtgca acttcacccg aacgatcctg gagcgggcgg
     2101 gccagagcgt gcagcaagac ctgaaagtcg tcagcctcga caccccggac ggccgccttg
     2161 tagcgttcga gacgcagatg ggcgagatcg ctaccaccgg cctggtgcag ggcgaccagc
     2221 tccagatcag cacaacctcg gccggccgca cgcagaagac gtcgatcccc tggcaggccg
     2281 agtggggcgg gctgttcgct gccgagcaat cggtccgccg gcagccgctc acgaccggcg
     2341 agcggcggac cgtccgcggc ctcgtgccga tgctgaacgt gccgggcgat acggttttgg
     2401 ccgccggcga cgatgaaacc gtggacctgc ccggcggccc gcggaagctg ctcaaggtgc
     2461 aagccacgat gcaaatcggc tcgcagcaga tcgaatcgac gcagtgggcc gacgagcggg
     2521 gcgagacgct aaaggccgtg gtccccgccg tggggcagga gacgctccgc acgacgaaag
     2581 acgacgccct gcggcggacg gccggcggca agctcgacct gctcgtcaac tcgacggtcc
     2641 cgctggcggg cggctttccc aatgcccagc ggacgaagaa ggccgtgtac ttggcgaacg
     2701 tgaagaatgg caagatcgac ggagttttct cgaatagcct ttcgcaacac gttgccatca
     2761 aggacgaccg cacggccgag gttaccgtca tcgccgtccg gccgaatgat cccaaggtag
     2821 caggcagggt cccgtcgccg acggccgacg accaatctcc cagcagctac atccaaagcg
     2881 acgaccccgc catcatccgc ctggccaact cggttgcgaa cggagagacc gactcctgga
     2941 caatcgcctg cgccctggaa aagcacgtcg attccaccat caagctgaag aacttttcac
     3001 aagccttctc gaccgccgcc gaggtcgccc gcacgctcga aggcgactgc accgagcacg
     3061 ccgtgctttt cgccgcactc tgccgagcgc ggaacatccc ggcccgctgt gcgttcggcc
     3121 tcatctactt cgagccgctc aagggctttg ccttccacat gtggaacgag gtctggatcg
     3181 gcgaccgctg ggtgcccatg gaccccacgc tcggccaagg cggcatcgct gccgaccacc
     3241 tccagctcgg cagcaccagc ctcagcggcc aggccgccct cgccgacctg ctcggcgtga
     3301 tgaccgcctt cggccggctc gaattgcaac tcgtggccgc ggaatagagt tccgagttca
     3361 aagttccggg ttcaaagttg aagcagacgc taaactccga acccagaact cggaactctg
     3421 aactcgaaac tcggaactcg aaactcgaaa ctgtgtctgt cactcccatc gtcgccaagc
     3481 tttcgcccga ccaggcgatc ttacgcaccg agcggatgac gctgcggccg ctgacgcagg
     3541 gcgatgcgcc catcgtgcag cagctggcca gcgaaaagga cgtggccagc acgacgcggc
     3601 tgattccgca cccgtacccg ccggggatgg cagcgacttg gatcgccgca ctgcccgagc
     3661 tctatcagcg ggcggagatg atcgtctggg gcgtcgcgat ggacgagggg ccgctgctgg
     3721 gcacggtccg cctcacgctc taccccgtcg ataaccacgc cgaaatgggc tactggatcg
     3781 gcaagccgtt ctggaacaac ggctactgca ccgaagccgc ccgggccgtc gtctcctacg
     3841 gcttcgacgt gctcggcctg gagcgaattt acgccaacta cctggcccgc aacccggcca
     3901 gcggccgcgt cctgcacaag ctgggcatgc aacaggaagg cctccttcgt cgccaccgcc
     3961 gcaaattcgg ccgctacgaa gacctcatcg tctgcgggct cttgaagagt gaataccaga
     4021 agctgaaaag acgccccgcc agcccgcccc gcacggatgt ccaacccgcg ctgcagccag
     4081 aggccagcgt gcctccggct ggctaatttc gaggttgaaa tggagcccgt attgctcatt
     4141 cgccgcgaat tgatgagcaa ctatgaatac caattcgcgt cgcggcactt tccggtcgtc
     4201 gagagccgcg tgctctgcgg cgagcggttg gtcatcggcc gctattcggt gctgccgctg
     4261 tatgctgagc tggatcgcga cgtgaagctc ctcggcagcc gcctgatcaa cagcttcgag
     4321 cagcatcatt gggtcgccgc gttcgagtac tatcgcgatt tgaaagaatt cacgccggaa
     4381 acttgggacg acgacacgat ctatcagtgc cggcaccagg gtccgttcgt cgtcaagggc
     4441 aagacgaagt cgcgcaagtg gcaatggaag acgcacatgt ttgccgcgac caagagcgaa
     4501 gcgcttcaat tgggccagcg cctgaaagac gacgccgaaa tcggcgagca gggagtcgtc
     4561 tatcgccgct acattccgct gcgcactttc ggcctcggca aggacgggct gccttttacc
     4621 aacgagtggc ggttcttcta ttttggcgag cagcgcctga gctatgccta ttactggccg
     4681 ggcagcgact acctgcagaa ggcctccatc ccggccgcgg ccgtcgcctt agccgatcga
     4741 atagcagcga tcacggcgcg gttcgtgcaa ttcttcacgc tcgacatggc cgagacggaa
     4801 gccggcgaat ggattctgat cgaggtcaac gacgggcagg cctccgtccc gtcggagcac
     4861 gacttggacg agctctacgg caacctgcgg caggcagtgg ctgcgaatgc ggcgcaagca
     4921 tgattacacc aagaaaatct cccgcagcga ttcccgcagc gtggccgcgt cgacgagcag
     4981 ggcatcgacg ctatcttcga gcggcgtttg ccggggccgc tgcgtgactt cgtctacgaa
     5041 cgatcgcacg ccctgccgga acgtgttgat cgcagccaag gcttgatcga ctgccgatga
     5101 gatttcctcc ggactcagaa ctcccgccgt accgccgctc gtgcccgtgg tgctcgcgaa
     5161 gctggagctt acggagcctg gcggggcgaa cggatcggcc gcggcgatgt tcacgctgta
     5221 acccaaatcg gccagcgacg ccacggtgat ccggctgatc ggcaggctcg tcccggggcc
     5281 gatccagccg gtcatgatct cgtt
//
LOCUS       DASRJB010000834         6360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1936145, whole genome shotgun sequence.
ACCESSION   DASRJB010000834 DASRJB010000000
VERSION     DASRJB010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936145"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1245)
                     /gene="tdh"
                     /locus_tag="VFB80_21930"
     CDS             complement(124..1245)
                     /gene="tdh"
                     /locus_tag="VFB80_21930"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZN36511.1"
                     /translation="MNSSLPRTMVAIRKLKNEPGLWLQEDTPTPAIGPRDVLVAVTHA
                     GICGTDRHIYEWDAWSKSRVVVGITTGHEFVGRVVAVGSAVTRTTVGQRVSAEGHIGC
                     GICQPCRTGNGHICEKVDILGIDTNGCFAEYVAVPEENIWPVHPDIPDHIAAIFDPLG
                     NAMHTVMAAGVSGRSVLITGVGIIGLMAVTIARAAGAGKILVADVSPRRLELAKGLGA
                     DVAYDSRNENWAQKARAETHDQGPEVFLEMSGNPKAIRQGFSALRNGGTAALLGIPAE
                     PVALDLPNDIIFKGATVLGINGRKMFETWYQVENFLLSGRLHLDQIVTHQLPLAEFER
                     GLKLMQAGEAIKVVLEVPGSKATRSQAPLGTARLEAPLR"
     gene            1349..1975
                     /locus_tag="VFB80_21935"
     CDS             1349..1975
                     /locus_tag="VFB80_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN36512.1"
                     /translation="MAAATKRNAAKPPPSTAEPLSQVVCDRVRSLRKSKGWTLEQLAS
                     LSGVSRSMLSQIERRAANPTLGVAFRIAQAFGMTLGDLVESPQPGPRIDVIRASDRGS
                     LFRDDEQCRIRTLSPLHLEKEVEFYELTLKPGGSLVSAPHVSGTREFLAIETGAVRVT
                     SATETVEVQPGDSAHYPADVPHRIDNLSNGEAVAFLVVIYERAPLRRG"
     gene            2057..3409
                     /gene="hisD"
                     /locus_tag="VFB80_21940"
     CDS             2057..3409
                     /gene="hisD"
                     /locus_tag="VFB80_21940"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZN36513.1"
                     /translation="MSTPFSIQRLDSRQGDIRPALDALRQKLSPAGNVVSEAGRRRTI
                     EVFGEPLSPQQVVERICGDVKSRGLSAVLDYSRKLDKAELTAQSVRVSPVELKQAHAA
                     ADAEFLATIRRIRANILQFQQAILHRDVEVKRPGVILRQRYSPLARVGICVPGGAAAY
                     PSTVLMTAVPAQAAGVGEIAVVAPPTRFGANNPVVLATCHELGIREVYRIGGAQGVAA
                     LAYGCEGVPKVEKIVGPGNLFVALAKRHVYGEVDIDSIAGPSEVVVIADGSTRPDFTA
                     ADLLAQAEHAPGASILITWSEKTLDATAMELERQVASLARCEETIQSLRDFGCLILVR
                     DEDEACRLTDLIAPEHLHIAIDGASRLLPKIKNAGATFLGNYSPVALGDYAAGPSHVL
                     PTGGTARFASGLSANQFLRSNSVIDYSAEALQAIAPDVMRMADVEGLTAHRASVEVRT
                     "
     gene            3444..4490
                     /gene="hisC"
                     /locus_tag="VFB80_21945"
     CDS             3444..4490
                     /gene="hisC"
                     /locus_tag="VFB80_21945"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZN36514.1"
                     /translation="MYARPEIAAMDGYTPGEQPQAGKFIKLNTNENPYPPPPGIRGAI
                     EDVLARGLQKYPDPMASAFRTRAAELLGLPSRDWVLCGNGSDDILTICTRTFVGQGDL
                     LRLPYPSYILYRTLAQLQAARWEEVRFSSDWSLPAAFGQAAPQLKLAFLPNPNSPTGT
                     MVSREAILELADRLPCPLLVDEAYVDFADFHCADLVTRNEKIMVSRSLSKSYGLAGLR
                     FGYLAAQPQLIRELVKVKDSYNCDALSIAAATAAINDTAWLADNRRKIVATRGRLESG
                     LRQLGFDSVPSQANFVWCTHPRRSAKELYEQLKAAHVLVRYMNYPGWADGLRISVGSD
                     EQIEALLGLLKGMV"
     gene            4531..5118
                     /gene="hisB"
                     /locus_tag="VFB80_21950"
     CDS             4531..5118
                     /gene="hisB"
                     /locus_tag="VFB80_21950"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZN36515.1"
                     /translation="MPRTAKIDRATRETQIKLELNLDGSGAFAGRTGIGFFDHMLELL
                     ARHGALDLKVEAQGDLHVDQHHTVEDVGICLGQAVKQALGDKAGIRRYGHFTLPMEET
                     LCTTAIDLSGRYYLVFGAEFASPKIGEFDSELVEDFWQAFAANALCNLHVLVHYGRNS
                     HHVAEAIFKSAARALRMAVEPDPRMPGVPSTKGTL"
     gene            5131..5826
                     /locus_tag="VFB80_21955"
     CDS             5131..5826
                     /locus_tag="VFB80_21955"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN36516.1"
                     /translation="MSSPDAPQPSSFAESSAAERELHPTERRPRLWPWLVLAAMIAAL
                     IAVRTFAPPPAEPDGNGRRHPAVGKEITQVSLQPLTGGGPVILEADLVGKVTAINFWA
                     TWCGPCKMEFPELIELEEDFRSRRDFQFLSVSCSGPDDVEQLRQVTAAFLRQQRAEFR
                     TLHDPGFQTQLALAAAASLDGFAYPTTVVIDRQGVIRGLWVGYAPGDERRVRAAIESA
                     LRNEPLPAAATPR"
     gene            5925..>6360
                     /locus_tag="VFB80_21960"
     CDS             5925..>6360
                     /locus_tag="VFB80_21960"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN36517.1"
                     /translation="MQRWLVCIFIGVLVAAAAAVRGAESDVDFLRDVKPIFSQRCFRC
                     HSSLEQESNLRLDSVPAILKGGDGGAVVVPGKGGESRLLAAVRRTGELKMPPEGPPLT
                     EAQIGVIAAWIEAGAPAPPAGAETKVAHWSFRKPIRPELPAID"
BASE COUNT         1083 a         2091 c         2064 g         1122 t
ORIGIN      
        1 tgggcatgga ataaacctgc cgctgagggt ctcaccagaa ttcccttggg ccgaagctcc
       61 gcttcccttg tgcccaagct ccgcttgggc acacattctt tcgaagctct gcttcgttta
      121 cggctatcga agcggagctt cgagtcgtgc ggtccccagc ggagcttggg accgagttgc
      181 ctttgaaccc ggaacttcga ggacaacctt gatcgcctcg ccggcctgca tcagcttcag
      241 gccgcgctcg aattccgcga gcggcagctg gtgcgtaacg atctggtcga ggtgcaggcg
      301 accggacaag aggaaattct caacttgata ccaggtctcg aacatcttgc ggccgttgat
      361 cccgagaacc gtcgcgccct tgaagatgat gtcattcggc aggtccaagg cgactggttc
      421 ggccggaatc ccgagcagcg ccgcggtgcc gccgttcctc agggcggaga agccttggcg
      481 gatggccttg gggttgccgc tcatttcgag gaagacctca gggccctggt cgtgcgtttc
      541 cgcgcgggcc ttctgcgccc agttctcatt tcgcgaatcg taggccacgt cggccccaag
      601 acccttggcc aattccagac gccgcgggct gacatcggcc acgagaatct tcccggcccc
      661 ggccgcgcgg gcgatcgtca ccgccatcag gccaatgatg ccgacgcccg tgatcagcac
      721 actccgcccg ctgacgccgg cggccatcac cgtgtgcatc gcgttgccca acggatcgaa
      781 aatcgccgca atatgatccg gaatgtctgg atggaccggc cagatgttct cctccggcac
      841 cgccacgtat tccgcaaagc agccgttggt gtcgatcccc aggatgtcca ccttctcgca
      901 gatatgcccg ttgccggtgc ggcacggctg gcagatgccg cagccgatgt gcccctcggc
      961 gctgacccgc tggccgacgg tggtccgcgt caccgcgctg cccaccgcga cgacgcggcc
     1021 cacgaactcg tgaccggtgg tgatgccgac gaccacgcgg ctcttgctcc aggcgtccca
     1081 ctcatagata tgccggtccg taccgcagat gccggcgtgc gtcacggcga ccagcacgtc
     1141 gcgggggccg atggccggtg tgggcgtgtc ttcctgcagc cacaagccgg gctcgttttt
     1201 gagcttgcgg attgcgacca tcgtgcgcgg cagactggaa ttcatgagga ggccgtttct
     1261 tatttcctgg aattcggaac gctcatccgc tatagtagag agccttgtct gttttgcaag
     1321 acgagaattc cattattttg gagggcggat ggccgccgcc acgaagcgaa acgctgcaaa
     1381 accgccgccg tccaccgctg agccgctcag ccaggtggtt tgcgaccggg tgcgcagcct
     1441 gcgcaagtcg aaaggctgga cgctggaaca acttgcatcg ctttcgggcg tcagccgcag
     1501 catgctcagt cagatcgagc ggcgagcggc caaccccacg ctcggggtgg cattccgaat
     1561 cgcccaggcg tttggaatga cgctcggcga cctggtcgag tcgccgcagc cggggccgcg
     1621 gatcgacgtg atccgcgcgt cggatcgggg ctcactgttt cgcgacgacg agcaatgccg
     1681 gattcgcacc ctgtcgccgc tgcacctgga gaaagaggtc gaattctacg agctgacgct
     1741 gaaacccggc ggttccctgg tcagtgcccc ccacgtctcg ggcacgcgcg agtttttggc
     1801 gattgaaacg ggagcggtgc gagtaacctc cgccaccgag acggtcgaag tgcagcccgg
     1861 cgactccgct cactatcccg ccgatgtgcc acaccggatt gacaatttga gcaacggcga
     1921 agcggtggcg tttctggtgg tgatttacga gcgagcgcca ctgcggcgcg gctaacttgc
     1981 ggccctttcg gtcttttcgc cgtacgaaca aaatgggacg tttgagtgtc ctgctgccag
     2041 cgagtcttcc atcttgatga gcaccccgtt ttccatccag cgcctcgaca gccgccaagg
     2101 cgatattcgc cccgcgctcg atgcgctgcg ccagaagctc agcccggcgg gcaacgtggt
     2161 cagcgaagcg ggtcggcggc ggacgatcga ggtctttggc gaaccgcttt cgccgcagca
     2221 ggtggtggag cggatttgcg gcgacgtgaa gtcgcgcggc ctgagtgccg tgctcgatta
     2281 cagccgcaag ctcgataagg ccgagctgac ggcgcagtcg gtgcgcgtgt cgcccgtcga
     2341 attaaagcag gcacacgccg cggcggacgc ggaattcctg gcgacgatcc gccgcattcg
     2401 ggcgaacatc ctgcaatttc agcaggcgat tttgcaccgc gacgtagaag tgaagcggcc
     2461 cggcgtgata ctgcggcagc gctattcgcc gctggctcgc gtgggcattt gcgtgcccgg
     2521 cggcgctgcg gcatatcctt cgacggtcct gatgacggcc gtgccggctc aggcggcggg
     2581 cgtcggcgag atcgccgtcg tcgctccgcc gacgaggttc ggcgccaaca acccagtcgt
     2641 gctggcgaca tgccatgagc tgggcatccg cgaggtctat cgtatcggcg gggcgcaggg
     2701 cgtggccgca ctggcttacg gctgcgaagg cgtgcccaag gtcgaaaaga tcgtcggccc
     2761 cggcaacctg ttcgtcgctc tggccaagcg acacgtctat ggcgaagtcg atatcgattc
     2821 gatcgccggc cccagtgagg tcgtcgtcat tgcagatgga tcaacgcggc ccgacttcac
     2881 cgcggcggac ctgctggcgc aagccgagca cgctcccggg gcgagcattc tcatcacctg
     2941 gagcgagaaa acgctggacg ccaccgccat ggaactggag cggcaggtcg ccagtctggc
     3001 gcggtgcgaa gagacgattc aatcgctccg cgatttcggc tgcctgattt tggtgcgcga
     3061 cgaggacgaa gcctgccgcc tgaccgacct gattgctccc gagcatctgc acatcgccat
     3121 cgacggcgcg agccgcctgc tcccgaagat caaaaacgcc ggcgcgacgt tcctcgggaa
     3181 ctacagcccg gtcgccctcg gcgactacgc cgcgggcccg tcgcacgtcc tgcccaccgg
     3241 cggcaccgcc cgcttcgcct ccggcttgtc cgccaaccag ttcctccgca gcaacagcgt
     3301 cattgactat tcggccgaag ctctgcaggc gattgccccc gacgtgatgc ggatggccga
     3361 cgtcgaaggc ctgacggcgc accgggccag cgttgaagtt cgaacctgac tcctgactcg
     3421 cgactcctga cccctgaccc ctgatgtacg cccgccctga aattgctgcg atggatggtt
     3481 acacgcccgg cgagcagccg caggcgggga agttcatcaa gctgaatacg aacgagaacc
     3541 cgtatccgcc gcccccgggc attcgcgggg cgatcgagga tgtgctcgcg cgggggctgc
     3601 agaagtatcc cgatccgatg gccagcgcct tccgcacgcg ggccgcggag ttgctgggcc
     3661 tgcccagccg cgactgggtg ctctgcggca acggcagcga cgacatcctc accatctgca
     3721 ctcgcacgtt cgtcggccag ggcgacctgc tgcggctgcc gtaccccagc tacatcctct
     3781 accgcacgct ggcccagctc caggcggccc gctgggaaga ggtgcggttc agctccgatt
     3841 ggtcgctgcc cgcggcgttc ggccaggccg cgccgcaact gaagctcgcc ttcttgccca
     3901 atcccaacag cccgaccggc acaatggtca gccgcgaggc gatcctggag ttggccgatc
     3961 gcttgccgtg cccgctgctg gtcgatgagg cctatgtcga tttcgcggat ttccactgcg
     4021 ccgacctggt cacgcggaat gaaaagatca tggtcagccg ctcgctcagc aagtcgtacg
     4081 gcctggcggg gctgcggttc ggttaccttg cggctcagcc gcaactcatt cgcgagctgg
     4141 tcaaagtcaa ggattcgtac aactgcgacg cgctgtcgat cgcggcggcc accgcggcga
     4201 tcaacgatac ggcctggctg gccgacaatc gccgcaagat cgttgccacc cgcgggcggc
     4261 tggaaagcgg ccttaggcaa ttgggcttcg actccgttcc ctcgcaagct aactttgtct
     4321 ggtgcacgca cccgcggcgc tcagcgaagg agctgtacga gcagctgaag gcggctcacg
     4381 tgctggtgcg gtacatgaac tatcctggct gggccgacgg cctgcgaatc agcgtcggca
     4441 gcgacgagca gatcgaggcc ctgctggggt tgctgaaggg gatggtttag cacgcaattt
     4501 cagatttcaa atttcaaatt ccagatttgc atgccacgaa ccgccaaaat cgaccgcgcg
     4561 acgcgcgaga cgcagatcaa gctggagctt aacctggatg gcagcggcgc gttcgccggc
     4621 cggacgggca tcggcttctt cgaccacatg ctggagctgc tcgcgcggca cggggcgctg
     4681 gacctgaagg tcgaggccca gggagacctg cacgtcgatc agcatcacac cgtcgaagac
     4741 gtcggcatct gcctgggcca ggcggtcaag caggccctgg gcgacaaggc gggcatccgc
     4801 cgctacggcc acttcacgct gccgatggaa gagacgctct gcacgacggc gatcgacctc
     4861 tccggccgct actacctggt cttcggggcc gagttcgcca gcccgaaaat cggcgaattc
     4921 gacagcgagc tggtcgagga cttctggcag gcgtttgccg ccaacgccct gtgcaacctg
     4981 cacgtgctgg tccactacgg ccgcaacagc caccacgtcg ccgaggcgat cttcaaatcg
     5041 gccgcccgcg ccctgcgaat ggcggtcgaa cccgacccgc ggatgcccgg cgtgccgagc
     5101 acgaagggaa cgctgtaaag gcggctcgcg atgtccagtc ccgatgctcc gcagccgagc
     5161 tccttcgccg aatccagcgc ggcggagcgc gaactccatc ctacggaacg ccggccacga
     5221 ttgtggccgt ggctcgtact ggcggcgatg atcgcggcac tgattgccgt tcggacgttc
     5281 gctccgccac ccgcagagcc cgacggcaat ggccgccggc atccggccgt gggcaaagag
     5341 atcacgcagg tctcgctgca gccgctaacc ggcggcgggc cggtaatttt ggaagcggac
     5401 ctcgttggaa aggtgacggc gatcaacttc tgggcgacgt ggtgcgggcc ctgcaaaatg
     5461 gaattccccg agctgatcga gctggaagaa gactttcgct cgcgccgcga tttccagttt
     5521 ctctccgtgt cgtgtagcgg accggacgac gtcgaacagc tgcggcaagt gaccgccgcg
     5581 ttccttcggc agcagcgggc cgaattccgc acactgcacg acccgggctt tcagacgcag
     5641 ctggctctgg cggcggccgc aagcctggac ggcttcgcct atcccacgac ggtcgtcatc
     5701 gatcgccagg gcgtcattcg gggcttgtgg gtcggctatg ctcccggcga cgaacgccgc
     5761 gtccgggcgg ctatcgagtc cgccctccgt aatgaaccgc tgccggctgc ggcgacacct
     5821 cgctaggccc gccgccagcg gcctgaatct gcttgcgacc gggctctggc cgcggctagg
     5881 atcagcaatg gttgatagat ggtccgcatt gtcgagggaa gctcatgcag cgctggcttg
     5941 tgtgcatttt cattggtgtt ctggtcgctg ccgcagccgc cgttcgcggc gctgaatctg
     6001 acgttgattt cctccgcgac gtcaagccga tcttctcgca gcggtgcttc cgctgccaca
     6061 gctcgctgga gcaggagagc aacctccggc tggacagcgt gccggcgatt ctgaagggcg
     6121 gcgatggggg cgcggtcgtc gtgcccggca agggcggcga gagccgtttg ctggcagcgg
     6181 tgcgtcggac gggcgagctg aagatgccgc cggagggccc gccgctgacc gaagcgcaga
     6241 ttggcgtgat cgcggcctgg atcgaggccg gagcgcccgc cccgccagcc ggcgcggaga
     6301 cgaaggtcgc ccattggtcg ttccggaagc cgatccggcc cgagttgccg gccatcgacg
//
LOCUS       DASRJB010000835         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1936153, whole genome shotgun sequence.
ACCESSION   DASRJB010000835 DASRJB010000000
VERSION     DASRJB010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936153"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..436
                     /locus_tag="VFB80_21965"
     CDS             113..436
                     /locus_tag="VFB80_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36518.1"
                     /translation="MPTRREKIEAMLMAEPRDGFLRYSLACEYDNEERFDESWKLFEG
                     LMADRPPHVASFLRGAQLLVKLDRIADARAVLRTGIEAARQQGEAHAAGEMGELLASL
                     GSLGE"
     gene            628..2895
                     /locus_tag="VFB80_21970"
     CDS             628..2895
                     /locus_tag="VFB80_21970"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN36519.1"
                     /translation="MLSRCLLVAVVVGSVTVARAQDSLPQVKDVERQPLVAQVKRLAE
                     ALDYLGVPLSNADRAALDAAGNEMDSAAAAASIQKVLDPHCLAGVVLKSDKEITAAAG
                     PAAKKLVEQGWTQFLVKVHNAAGLTGELRAASPNALRLAGSPPAEVERRWLDLQMFNA
                     QPLVPKLSGLPLEYRIVQLYSRDAGQREAALALEYVVRPGTPPMRADAPRGTPEEVIR
                     SRPLPLAFECAAAHEVTFRVLDEMGQPTTAAFTVRDSQNRVYPSQAKRLAPDFAFHPQ
                     IYRGEGEKLKLPAGKYTISCQRGPESIPETKELTVGDGPATFGYQVKRWIDPAQYGWI
                     SGDHHIHAAGCAHYSNPTEGVHAQDMIRHCLGEDLKVGCNLTWGPCFDYQLQFFTGKD
                     DVVSKYPYLLRYDIEVSGFGSHRTGHLCLLRLKERNYPGGDSNDHWPTLGLSTLRWAK
                     KQGAVTGPAHSGSGLARSQDRVEGADGPSGLPNYFIPQYNGIGANEYIVDITHELPGP
                     DGKPLPAIDFISTMDTNRQQELNMWYHTLNCGFRVRASGETDFPCISGQRVGMGRVYV
                     KTDGKLNYEDWCEGIRQGRSYVSDGTSHLLDFIAATTAGLEVTVPVGTKGSEIKLAAP
                     QKIRLYAKVAARLDGKTVPVEAVVNAFPVDKKEIVADGSLQVVSFEVPVERSSWVALR
                     LYPSVHTNPVFVLVGDKPIRASRRSAEWCLRGVDRCWQEKQQFYAAAEKDEAAKAYEH
                     AREVYRQIVKESDVD"
BASE COUNT          547 a          979 c          999 g          464 t
ORIGIN      
        1 ggccgaatgg cgccgacatt gcgcccgaga ccttgtacga cgcgccagtg atagagaaga
       61 cgaagactca gcgccccaag cgcgcaaaaa tcggtcgccg ctcatagatc ccatgcccac
      121 tcgccgcgaa aagatcgaag cgatgctcat ggcagagccg cgggacggat tcctgcggta
      181 cagcctggct tgcgagtacg acaacgagga gcggttcgat gaaagttgga agctgttcga
      241 gggcctgatg gcagaccggc cgccgcacgt cgcgtcgttc ctccgcgggg cgcagttgct
      301 ggtcaagctc gaccgcattg ccgacgcccg cgcggtcctc cgcaccggca tcgaagccgc
      361 ccggcaacaa ggcgaagccc acgccgcggg cgagatgggc gagttgctcg ccagcctcgg
      421 atcgctgggc gagtgaacta tgtaggccgc ctgccgggcg ggacctgggc cacgcaaggc
      481 tcgtcaatcg cgtcgcgctt ccgttgccag gtcgctgcgc ttccctcatg gccccttccg
      541 ccagaagggg cctactagtc gcgtgcgctt gcgcggaggc gtaacatgat cggctgaaat
      601 ccaccttctt tcgcgagggg ccctaccatg ctcagccgct gcctgctcgt cgccgtcgtt
      661 gtgggatcgg taactgtcgc gcgtgcgcag gacagcctgc cgcaggtcaa ggacgtcgaa
      721 cggcagccgc tggtggcgca ggtcaagcgg ctggcggagg cgctggacta tctgggagtg
      781 ccgctgagca acgcggaccg cgcggccctg gatgcagccg ggaatgaaat ggactcggcg
      841 gcagccgcgg cgtcgattca gaaggtgctc gatccgcact gcctcgccgg cgtcgtgctg
      901 aagtcggaca aggaaatcac ggcggcggcc ggaccagccg caaagaagct cgtcgagcaa
      961 ggctggacgc agttcctggt caaagtacac aacgccgcgg gactcaccgg cgaactgcgg
     1021 gcggcgagcc ccaacgccct gcggctggcc ggttcgccgc cggcggaggt cgaacgccgc
     1081 tggctcgacc tccaaatgtt caatgcccag cccctcgtgc cgaagctgag cggcctgccg
     1141 ctggaatatc gcatcgtgca gctctacagc cgcgacgcgg gccagcgcga ggcggcgctc
     1201 gcgctggaat atgtcgttcg cccaggcacg ccgccgatgc gggccgacgc tccgcgcggg
     1261 acgcccgagg aagtgatccg cagccggccg ctgccgctgg cgttcgaatg tgcggcggct
     1321 cacgaggtca cgttccgcgt gctcgacgag atggggcagc cgacgacggc ggcgttcacc
     1381 gtccgcgaca gccagaaccg cgtctatccg tcgcaggcca agcggctggc gcccgacttc
     1441 gccttccatc cgcagattta ccgcggcgag ggcgagaagc tgaagctgcc cgcgggcaag
     1501 tacacgatct cctgccagcg cgggccggag tcgatccccg agacgaagga actcacggtc
     1561 ggtgacggcc cggccacttt tggctatcag gtcaaacggt ggatcgatcc ggcacagtac
     1621 ggctggatct ccggcgatca ccacattcat gccgcgggct gcgcccacta ttcgaatccg
     1681 accgagggcg tccacgccca ggacatgatc cgccactgcc tgggcgagga tctcaaggtg
     1741 ggctgcaatc tcacttgggg tccgtgcttc gactaccagc tgcagttctt caccggcaag
     1801 gacgacgtgg tctcgaagta tccgtacctg ctgcggtacg acatcgaggt ctccggcttc
     1861 ggatctcacc gcaccgggca cctgtgtctc ttgcggctga aggagcggaa ttatcccggc
     1921 ggcgattcga acgaccattg gccgacgctg ggcctcagca cgctccgctg ggcgaagaag
     1981 cagggcgcgg tcaccgggcc ggcgcactcc gggtcgggcc tggcccgctc gcaggaccgc
     2041 gttgagggag cggacggccc gagcggcctg ccgaactatt tcatcccgca atacaacggc
     2101 atcggggcca acgagtacat cgtcgatatc acgcacgagc tgcccggccc cgacggcaag
     2161 ccgctgccgg cgatcgactt catctcgacg atggacacca accgccagca ggagctcaac
     2221 atgtggtacc acacgctgaa ctgcgggttc cgcgtgcggg ccagcggcga gacggacttc
     2281 ccgtgcatct ccggccagcg ggtcggcatg ggccgcgtct atgtgaagac cgacggcaag
     2341 ctgaactatg aggattggtg cgagggaatt cgccagggcc gcagctacgt cagcgacggc
     2401 acgagccacc tgctggactt catcgccgcc acgaccgccg gcttggaggt gacggtgccg
     2461 gtcggaacca agggcagcga gatcaagctg gccgcgccac aaaagatccg cctgtatgcc
     2521 aaggtcgccg cccgactcga cggcaaaacg gtgcccgtcg aggcggttgt gaatgcattt
     2581 cctgtcgata agaaagagat cgtcgccgac ggttcgctcc aggtagtctc gttcgaagta
     2641 ccggtggagc gcagcagttg ggtggccctg cggctctatc cgtcggtgca caccaacccg
     2701 gtattcgtac tcgtcggcga caagccgatc cgcgcctcgc ggcgcagcgc cgaatggtgc
     2761 ctccgcggcg tcgatcgctg ctggcaggag aagcagcagt tctatgccgc cgccgagaag
     2821 gacgaagcag ccaaggccta cgagcatgcc cgcgaggtct atcggcaaat cgttaaggag
     2881 agcgacgtgg attagtgccc ttagccggga gcaaacctgc tcccggtgat ccgtaagacg
     2941 gtgcaccacg cggactcggg gaccagattg gtcccggcta atggctgca
//
LOCUS       DASRJB010000836         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1099055, whole genome shotgun sequence.
ACCESSION   DASRJB010000836 DASRJB010000000
VERSION     DASRJB010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099055"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..737
                     /locus_tag="VFB80_21975"
     CDS             306..737
                     /locus_tag="VFB80_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36520.1"
                     /translation="MKTLTTRILLAAAATAAMFVSASSAEAFWGHHRATTAYSLPSVP
                     VVVGYPVAVARPVVVASPVVTASYAPAPVTTYYAPAPVTTYYAPAPAPVTTYYAPAVA
                     PVTSYYAPAVAPVTTYYAPAVAPVTTYYAPAVVAPRGWHHW"
     gene            complement(770..1489)
                     /locus_tag="VFB80_21980"
     CDS             complement(770..1489)
                     /locus_tag="VFB80_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36521.1"
                     /translation="MENKPAPAAAPPAAPAKPPSSGSTLRLLILLGVLLLCVGALAYD
                     YLILKPSCDKAYADIQSLVDERNKQGVKDGKLVTSEDIQKLVGFSPTWTKEEDKDGYT
                     IEHYCWWGKVPLLSRRRHFIAIVYTGNKPRHFSSHYQEEPPVEALPLMQTPPPVDPNA
                     PPLTLEGATVLGSGSENKGKDEPGKTPDSTDKPTTPEPPAEKSKADETPKPADTKPAD
                     AKAADSKPAETKPEEKAKDNP"
     gene            1714..3288
                     /locus_tag="VFB80_21985"
     CDS             1714..3288
                     /locus_tag="VFB80_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36522.1"
                     /translation="MSPQTPVPKPRRRWLPQFSLGAMFVLVTAAAIGVWYWYQWPYEV
                     RNVTYYPQAVDPFAPTGAKPAGPAQVLQLEVEYVRRVWSRDRQTIRHGPRRVYDGSDV
                     LLHEGGYRNGVKHGRFADYYKGQLQSEAFFSRGQLHGASRRWNNKGQLVEETNYDRGQ
                     RHGRYLRSSYAGQREVEGYFDHGAPSGQWQWSPQPSRRGSRPAGSVAGQWQGGRPDGV
                     WVCRNAQGAVCATLEFDRGRLLKSTAGTFDQRLQELLAAGAIHDPEVILRLLNPASAE
                     FDATPLAAAVADLNDALQSQILVDQRHRVSLAPRRAAADSRPASYPVRLSTETTILVP
                     LDERAPIYQYDLALPPSAERPPDPPRVVGLVDVPLTDRFDNLPLIVALEKLLSPYGLA
                     CDYRYGVLWIDRADSVRNWKDSTGISQIQPPRGSRLAEMWEQPVPVGVEFVMTPVIDA
                     LTYMQDQHAPGLKFDLSGIPIPTSRPLPQRQLRPVTLNVRNISLQHSIGLILDLADCQ
                     ATLQGETIVVSAEPIP"
     gene            complement(3308..4222)
                     /locus_tag="VFB80_21990"
     CDS             complement(3308..4222)
                     /locus_tag="VFB80_21990"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HZN36523.1"
                     /translation="MQITRRSAIVGAATLPAAVAAGVWAVAPPASCRGSSEISIAIAG
                     EKRVVTANGLPGHVVGNFPNPHNPAPILPQRHRFELPLEPKAADKPSSVGTWIFGVAV
                     NGVPFDPSGPFWNADATSGWQFEVMHPANAIALGIDVNHAHTQGRRGMYHYHGLPTGL
                     LWDLTAAESDKPMQLLGYAADGFPIYGPVCPSSPSDLKSHPRRLRSSYRLRSGSRKDG
                     PGGRYDGTFVQDFEYDPMRSELDECNGRSGPTPEYPHGTYYYVVTDEFPFIPRMYRGM
                     PDPSFRHGPPPGKSPPVPPELRRYEGKA"
BASE COUNT          707 a         1479 c         1431 g          801 t
ORIGIN      
        1 ttgggcatcg agcacgaccg cttcacctac cgctggcaag gccgcgactt ccggctgacg
       61 gatgtcaaag gccacgtggt gacgcagctt ctggcctgac cgcgcggatg cttccgccgc
      121 tggcgcgtcc ctgatgtcac aagctgttac gaataagcag cctgggcaag ctgttaggcc
      181 gcccgcagct caagattccc gtagaaaaag cagtcccggc aactgaccgc cagaagcaac
      241 tcagcatata tttcagttgc gcctcgtcca cgccgcacac ttcgcaccat cccagggaga
      301 gaccgatgaa gacgctgacc acgagaattc tgctggctgc tgcggcgacc gcagcgatgt
      361 tcgtcagtgc ctcatcggcc gaggcttttt ggggacatca ccgcgctacg acggcctact
      421 cgctgccgtc ggtcccggtc gttgtgggct atcccgttgc ggtcgctcgc ccggtagtcg
      481 tcgcttcgcc ggtcgtcaca gccagctatg cccccgcgcc ggtgacgact tactatgcac
      541 ctgctccggt caccacctac tacgcgcccg cgcccgcccc cgtgacgact tactatgcgc
      601 ccgcggtcgc gccagtcacc agctattacg cgcccgcagt cgcgccagtc actacctact
      661 acgcgccggc ggtcgcgccg gtcaccacct attacgcgcc tgccgtcgtg gcgcctcgtg
      721 gctggcacca ctggtaaccg cggtgccgta gcaagggcac gcgatcgcgc tacggattgt
      781 ccttcgcctt ttcttcgggc ttggtctcgg cgggcttgga gtccgctgcc ttggcgtccg
      841 ccggtttggt atccgcgggc ttgggcgttt cgtccgcctt cgatttctcg gcgggcggct
      901 ccggcgtggt gggcttgtcg gtcgaatcgg gcgtcttgcc gggctcgtcc ttccccttat
      961 tttccgaacc ggagcccagg acggtcgccc cttccagagt gagcggcggc gcgttgggat
     1021 ccacaggcgg cggcgtttgc atgagcggca gcgcctcgac cggcggctct tcctgatagt
     1081 gcgagctgaa atgccgcggc ttgttgcccg tgtagacaat ggcgatgaag tgccgccgcc
     1141 gcgagagcaa gggaaccttg ccccaccagc aatagtgctc gatcgtgtaa ccgtccttgt
     1201 cttcctcctt ggtccacgtc ggggagaagc cgacgagctt ttgaatgtcc tcgctcgtca
     1261 ccagcttgcc gtccttgacg ccttgcttgt tgcgctcgtc cacgagcgat tgaatgtcgg
     1321 cgtaggcctt gtcacagctc ggcttcagga tgagatagtc gtaggccagc gcgcccacgc
     1381 agagcagcaa gacgccgagc aaaatcaaga gccgcagcgt gctgcccgag gagggcggct
     1441 tggcgggcgc agcgggagga gcggcggcgg gggcgggttt gttctccata gacgcgtgta
     1501 ccttggatta agtgagtgcg attaggcaga gtccccaaac gtcggccaga atgaggcccg
     1561 gagtgcacca agtgtaaagc ggctgggggc aactttccag cggcaattcg cttaaccgga
     1621 gccggaggcg acggcgacta tcgacatcgg cgctgcacga aggaatacat tggtattggc
     1681 tcggactccc gtgcaatacc cgcggagcat gccgtgtccc cgcaaacgcc cgtcccgaag
     1741 ccccgccggc ggtggctgcc gcagttcagc ctgggggcga tgttcgtcct ggtgacggcc
     1801 gcggcgatcg gcgtctggta ctggtaccag tggccgtacg aagtgcggaa cgtcacctac
     1861 tacccccagg cggtcgatcc tttcgccccg actggcgcca agccggcggg gccggcccag
     1921 gtgctgcagc tggaagttga atacgtccgc cgcgtctgga gccgcgaccg ccagacgatc
     1981 cgccacggcc cgcggagggt ctatgacggc agcgacgtgc tgctgcacga aggcggctat
     2041 cgcaacggcg tcaagcacgg ccgctttgcc gactactaca agggccagct ccagagcgaa
     2101 gcgttcttct cgcgggggca actgcatggg gccagccgcc gctggaacaa taagggtcag
     2161 ctcgtcgaag agacgaacta cgatcgcggc cagcggcacg gccgctatct gcgctcgagt
     2221 tacgccggcc agcgcgaggt cgagggctat ttcgaccatg gcgcaccctc cggccaatgg
     2281 cagtggtctc cgcagccgag ccgccgtgga agccgtcctg ccggctcggt cgccggccaa
     2341 tggcagggcg gcaggcccga cggcgtctgg gtctgccgaa atgcgcaggg cgccgtctgt
     2401 gccacgctcg agttcgaccg cggccggctg ctgaaatcga cggccggcac gttcgaccag
     2461 cggctccagg agctgctcgc cgccggcgcg attcacgacc ctgaggtaat cctgcggctg
     2521 ctgaatccag ccagcgccga gtttgacgcg acaccgctcg cggcggcggt cgccgatctc
     2581 aatgacgcgc ttcaatcgca gatcttggtc gatcagcgcc accgcgtttc gctcgcgccg
     2641 cgacgggcgg ccgcggattc ccggccggct tcttacccgg tgcgcctctc gaccgagacg
     2701 acgattctgg ttcccctcga cgagcgtgcg cccatctatc aatacgacct ggcgctgccg
     2761 ccctctgctg aacggccgcc cgatccgccg cgcgtcgtcg ggctggtgga tgtgccgctc
     2821 accgacaggt ttgacaacct gccgttgatt gtggccctgg aaaaactgct ctctccttat
     2881 ggcctggctt gcgactaccg gtacggcgtg ctgtggattg accgggccga ctcggtccgc
     2941 aactggaagg attcaaccgg catcagccag attcagccgc cgcgcggctc tcgcctggcg
     3001 gagatgtggg agcagcccgt tccggttggc gtggagttcg tcatgactcc cgtcatcgat
     3061 gctctgacct acatgcaaga ccagcacgcc cccggcctga aattcgacct ttcgggcatc
     3121 ccgattccca ccagccgccc gcttccgcag cggcagctgc ggcccgtcac gctgaacgtc
     3181 cgcaacattt ctctccagca ctccatcggc ctgatactcg accttgccga ttgccaggcc
     3241 acgctccagg gcgagacgat cgtcgtctcg gcggaaccga ttccttgacg ccaggcgcga
     3301 gctcgcctca cgctttgcct tcgtaccgcc tgagctcggg cgggacgggc ggcgattttc
     3361 ccgggggcgg accgtgccgg aacgagggat cgggcatgcc ccggtacatc cgcgggatga
     3421 aagggaactc gtcggtcacc acgtagtagt aagtcccatg cggatactcg ggagtcggtc
     3481 cgctgcggcc gttgcactcg tcgagctcgc tccgcatggg gtcgtattca aagtcctgca
     3541 cgaacgtccc gtcgtaccgc ccgccgggac cgtccttgcg cgagccgctc cgcagccgat
     3601 aactcgatcg cagccggcgg gggtggctct tgaggtcgct gggactgctg gggcataccg
     3661 gaccgtagat gggaaacccg tcggccgcgt atccgagcaa ctgcatgggc ttgtccgact
     3721 cggctgcggt caggtcccac agcaggcccg tcggcagccc gtgatagtgg tacatccccc
     3781 ttcgcccctg cgtgtgggcg tggtttacgt cgatccccag cgcgatggcg ttcgcgggat
     3841 gcatcacctc gaactgccag ccgcttgtgg cgtccgcgtt ccagaacggt ccggagggat
     3901 cgaacggcac cccgttgacg gcgactccga agatccacgt gccgaccgac gacggcttgt
     3961 cggctgcctt gggctcaagc ggcagctcaa agcggtgccg ctggggaagg atcggggccg
     4021 ggttgtgagg gttgggaaag ttgcccacca cgtgcccggg cagtccgttg gcggtcacca
     4081 cgcgtttttc gccggcgata gcgatcgaaa tttcggacga gccgcggcag ctggcaggcg
     4141 gggcgaccgc ccagacgccg gccgccaccg ccgcgggaag ggtagcggct ccgacaatgg
     4201 cgctgcggcg tgtgatctgc atgccagggc tccaacgcaa cggatttgaa cgttctgcta
     4261 ccctaccaag atagcgagtg acggtgccgc ggtcagcggc agccctcagc ttgagcagtg
     4321 ttccgaccgt ccttacggcg acgccgccct tacccgcagc atcgccgcca aaaaggcgtt
     4381 cacgtcggcg gccgcttcga tgagcttggt cgtaggag
//
LOCUS       DASRJB010000837        13922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3911094, whole genome shotgun sequence.
ACCESSION   DASRJB010000837 DASRJB010000000
VERSION     DASRJB010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13922
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911094"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..617)
                     /locus_tag="VFB80_21995"
     CDS             complement(318..617)
                     /locus_tag="VFB80_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36524.1"
                     /translation="MSASAMVKRSNVESTFSMIKRKFGDAVRSRTDVAMVNETLCKVL
                     AHNLCVLIQEQCELGIETEFWAEKPVTPALPPVVDAIHVQPAEDAEFEFAALAFV"
     gene            1012..2253
                     /locus_tag="VFB80_22000"
     CDS             1012..2253
                     /locus_tag="VFB80_22000"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HZN36525.1"
                     /translation="MCDNLIFRLSAGVAVAVGAVLLGPSAAQAQVAREPKASGSAAFS
                     EAVARAKESSRPLVVFGVAEGCTRCAALKQGLSTQSELKLLMTQYVSVEVPFGGREFA
                     AIFEDIVRQDAKFRQAIGAPSVFIFTAKGDAVYAGPNNANGMLPGDEFKKLLIAGIEN
                     NGGLRGAVKSSPANNLATDLAKARKQWGENQPVAAAATISKYVSSDKADGGEVAELVK
                     LTGLNVAQSKMEEQLDSLVEQVSEKGRSLLQAAVTLASTGDATRSAVQLAELDRVFGA
                     YPSFAGKFDAAWKELLAKASVPNLKEQAEAIDKARQAESAAGPDQAIAAYERVSATYP
                     GTKAAELSQLRITQLRTPKSAVPRLWKSKSGQFSVTATLVAFDGKSAQLRTAEGKTIT
                     IALEALSAEDQQFLMSVKAPN"
     gene            2534..2962
                     /locus_tag="VFB80_22005"
     CDS             2534..2962
                     /locus_tag="VFB80_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN36526.1"
                     /translation="MKANKIVFPTDLSKTSTAALGWATVLARDTGATLVIVHVEEPPV
                     AYGGGELYYGMDVGDREKLQQALAGVRPADPGVKFVHKLLVGNPARAIVQTAKDEQAD
                     LIVMGTHGRTGLLRVLMGSVAEMVVREAQCPVLTVRQPAK"
     gene            complement(2968..3615)
                     /locus_tag="VFB80_22010"
     CDS             complement(2968..3615)
                     /locus_tag="VFB80_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36527.1"
                     /translation="MFGPQQRPTLSPQAAAAAERALGKIFWGTLLAVLDFWLSYTTNG
                     SGIRFDVLNDAVGMLLIAWGVYQLSAIWDEPAYRRTMSLCFCVSLLALGQAVLDHLVL
                     PWPPLFGVVSTLFSLVCLVCIFLFCAAMRTFCWLGRLDQAEASWRTSQLLFLILSLVP
                     SAIMHAAGWLYFAAAGRASLEIGPLAILLLIAAVVPLIHMFVSISRTRRALLAAP"
     gene            complement(3668..5266)
                     /locus_tag="VFB80_22015"
     CDS             complement(3668..5266)
                     /locus_tag="VFB80_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36528.1"
                     /translation="MSTPTELPACITRDNLTSPYFPSLSFARLIAVCTLSGIFMLTAF
                     NRLNHTDLWGHLNFGRWMAEHQALPATDPFATAPAATPVLQAAWLSQLAGYEVQTHFG
                     NEGLALGHALLVTLTAGIVMLAAVRRGAPALWAWAAGLAMFLLDLPIVGTIRPQLFGQ
                     LGLALVLLACAEMPRRTHPLLWLPVVAALWVNLHGSILMGIAVLGIYAASVSWTAWQE
                     AGGDAAKLLRDRRLYVVWGALLLVLAAACLNPHGPQLLARVLLFNEHAALSSISEWRP
                     LSPLSLTGGLMIVSVGLAALLARCSAKKWELYEIGLLLLFGLATLPAIRMLAWWAIVW
                     PVVCIPHAAAAWRKRLADRAGEPAQAGDEPTAMRTVLAMGFVFMTLLVAPPTFAIVAG
                     YGRGEGAITVTDTPIYVADEAVRRDLSGNIATPMDWSDFLVWKSGGRLKPLVHSHVHL
                     TELDTWRDYEAVFRGDEVWLHLLRGHHMQYVLVQKKRYPDLLKAILMEDRSGQGGVRI
                     IYQDQRCLLAEVLPAKPVAVKPAS"
     gene            complement(5288..6493)
                     /locus_tag="VFB80_22020"
     CDS             complement(5288..6493)
                     /locus_tag="VFB80_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36529.1"
                     /translation="MLARISRFVLPVLIVAGALAVYRMAAVGPQPAAVVLPRNMPTVI
                     APRINDPRVVTDAQLAAVLDRVLPPADPVSTNNFVHALRLWGPNADFGDAKIPSGRQL
                     RDYFLSDRAFRRWAGEQAPPLFFRGRDGIEVRSYDDRVTDRETSSFHTDDLVATLAET
                     GVPAGAPLVLRDGQARVGDLLTTALLRAHPEQLEYEWTTIAYARYVFPHPGWRNKYGE
                     KINVDQLVDQILKNPPGIGPCNGLHRLEALVVLNRADDEYRALQPKTKLKVLTALKWT
                     SDVLVRAQTAEGYWTRQWPRGAAASQPGKDNPATLHDKLLVTGHHLEWLALAPEEVQP
                     PRETVIRAAQWLARTLVEMDQKDLLEAYGPYTHAARALCLWRSTEPIDAWKKGTATAS
                     HQVAATSDK"
     gene            complement(6548..8137)
                     /locus_tag="VFB80_22025"
     CDS             complement(6548..8137)
                     /locus_tag="VFB80_22025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN36530.1"
                     /translation="MNPSILVFTRDQKLLFELGQQLAGQHAIVPCETLDALPQRVGDQ
                     RLSAVLVHLERNTLNGYSPARFIAELDEAIDQAPIYGLMDENCPPRLKKLAEKAIDDC
                     LDIPLDYGRLTRLLSEKQDVEGELSGFWSQMPHKELHGRSRSLVTFTPEMFDVMDEIK
                     VAARHNVTVLLIGETGSGKTFLARLIHELSDRREDRFCTVACGALPPDLIESELFGNV
                     KGAFTGADHNREGKFAAAGSGTLLLDEIDVLPLEQQAKLLRVIETGEYEQVGSNETQH
                     SQARLIVASNYNLEELVRAGRFRTDLYYRLNILNFRLLPLRQRPWDIEYLARKFAVEH
                     GRTHGIQLRSIEPAFLETLKAYDWPGNVREMENVVRRAVLYCHRGVLTVQDLPSSIRD
                     AANGLVESLPAASVDRVPPAIVEDPLFDHTLAALPPRVRVGPLGPQPPRMAMPQPALH
                     IPQPLAAARIGGQAEAHAAGATATAARLASSLEARVDVLERRIIQDCLARNNFRRKET
                     AAELGISRVTLYNKMKKFGLL"
     gene            8517..9017
                     /locus_tag="VFB80_22030"
     CDS             8517..9017
                     /locus_tag="VFB80_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36531.1"
                     /translation="MNAILLTVLALGGADAAIFGGGGGGGGEYIDGGCGDGGECDGGG
                     WHNKAGHGGWLGPMPQSCYWPSFGCYPSTPWMHRYPAFHGTYYRKAYNYRNYFDYPWH
                     AGLHEPTSHFSYHVPGEAPAAGGGGAVPRPTPDAAYAPRGGLRTATAEGRISDGISVT
                     PYKVQR"
     gene            complement(9130..11709)
                     /locus_tag="VFB80_22035"
     CDS             complement(9130..11709)
                     /locus_tag="VFB80_22035"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN36532.1"
                     /translation="MNWSVTLTAWLLDYALLATAVLAAAAALRGCFRDPAQRVALAWG
                     TWLGLVLLAIAIALPQWPRLALGSLTQAEVVALIDQQPATQVESPPAIVTELPALEVV
                     TPESTAIQPPSALHLRWEQLILGGWLAIGSFAAAWIGLGLWQTWLLVRRSLSAPRWVI
                     DELTKVTGPRRGPQVRISPQLHSAAAVGALSPRILLPAEAVDEARRPIVRAALAHEWA
                     HIRHGDLWLLALERWLVPLLAWQPLFWWLRRSVRLDLELLADAAAAGDEPVEYAEALV
                     AWAKAASSAPAGLAALSLWESPHTLSRRVTMLLDSRRPLSSGSRRWQFLLVAATLLLI
                     GGLSLVTLRPPTADGQEAQPPASEAAPSGSPDQTPTAILLQCVVLEVERAELTAVLPT
                     EARPQPDPETEITLKSPEEWKQRLAELKARPGTVILSRPQIVTLDGQLALVEIGGKSP
                     PQSPDESTADTGAATESKPSRLTIQLTPKLTLQDSKQQVVLALDAARVDLKLGPDGSP
                     VATQQKLATKAVVPLGQTVIVASRPQKLELKPLLVAVEVQLAQREEQADKPAAAQAED
                     LRRLVRQLERRLAERTAEAQAQREQSEALRKQLADLKAQLDQLAPVQTNTIIRLRNSS
                     AADVADALKLLLDEVRLLGGDAQVASIKWSVDEASNSLIVSAQSRHWDAIKTIIARLD
                     RPGQSNSPYPPAPQRQLGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQLPNELAPSS
                     RNPNSTLNPLHRPNVDEAARLTLADRETQLQLLKLDLQEAEIGLQAAQQDAERAEALR
                     KHGTSAISQAELAKHQFELKAAEIKVMRAKVKLDGMQRLMAQPQPEPQPEPRPVLTPR
                     PAR"
     gene            complement(11706..12119)
                     /locus_tag="VFB80_22040"
     CDS             complement(11706..12119)
                     /locus_tag="VFB80_22040"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN36533.1"
                     /translation="MADVAPTERELQALKVLWQLGQATVREITAAINERGDDLAYTTV
                     LSLLQVMEQKGLVGHTATGKVYSYFAKADRDRTFQRLARGFLERVFDGSLDEYVLHAL
                     EARRLSPEELRRLEQLIAAARPDKPSRRKRKEDSP"
     gene            complement(12206..13081)
                     /gene="rsmA"
                     /locus_tag="VFB80_22045"
     CDS             complement(12206..13081)
                     /gene="rsmA"
                     /locus_tag="VFB80_22045"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZN36534.1"
                     /translation="MSRFEEAGIRPQTRRGQNFLIDLNLLDLLVRTADVGPQDVVLEV
                     GTGLGALTTRLAQEAAAVVSVEIDARLQQLASEELAKAGATNVTLLSTDALQNKSTMH
                     PAVLAAVREHLARHPGSRFKLAANLPYNVATPILANLLTADPLPVSLTATIQKELADR
                     MIAAPGTKDYSALSIWMQALCEIAIVRVMPPQVFWPRPKVQSAIVHIVPSAAKRARLS
                     DVEFFHTLIRDVFCHRRKLLRGVLAGCFREQLGRPAIDALLAAEGIAPEARAEELPVE
                     TLIALAENLRGRLRS"
     gene            complement(13147..13455)
                     /locus_tag="VFB80_22050"
     CDS             complement(13147..13455)
                     /locus_tag="VFB80_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36535.1"
                     /translation="MDDELERIHRLFGALSEAKWRRACKPGVSAVDVLTEDVFVPSGA
                     DELAIWLELTSPENYDSLRRYVEYWQRREINDYAERLHQQLLFEATNRWEQWNALTGI
                     "
     gene            complement(13483..>13922)
                     /locus_tag="VFB80_22055"
     CDS             complement(13483..>13922)
                     /locus_tag="VFB80_22055"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZN36536.1"
                     /translation="TTFSRLTAGDGVNLERSLRLGDRLGGHLVTGHIDGVGSVIKFRD
                     EGEWATVTFRVPAALARQMAEKGSVAVDGVSLTLVSVGKGRFSVALIPHTLQNTTLGD
                     LRVGVLVNIETDLLAKYVQRQLAVDGERLPDRSYSDFAQADSP"
BASE COUNT         2430 a         4426 c         4681 g         2385 t
ORIGIN      
        1 gcggctttgc tacggaaata ggccaaaaca aatcattccg tatcaggcgc gtgaattacg
       61 ttccagcgtg aatttgtgac cacactgcct gcatcggtag cgaatgactt taggccaggg
      121 cctgcgtttc ctgccggccc cgctgtcgat gattttgatg ccacttggga agccgcgagc
      181 gaacacttcg cgagaggtgc atacggggca tgcaggaggt cgcggcactg gttgaatcgg
      241 gggcatgaat ccgattctac cagcccgcgc acgaacaagc cccgcctgat tcgacggggc
      301 aacttgacca gcggcagcta cacgaacgcc agtgccgcga attcgaactc ggcatcttcc
      361 gccggctgaa cgtgaatcgc atcgaccacc ggcggcagcg ccggcgtgac gggcttctct
      421 gcccagaatt cggtttcgat ccccagttcg cattgctctt gaatcaagac gcaaaggtta
      481 tgggcgagca ccttgcatag cgtctcgttg accatcgcca cgtcggtacg gctgcggacg
      541 gcatcgccaa acttccgctt gatcatgctg aacgtcgatt cgacgttgct ccgcttgacc
      601 attgctgacg ctgacaccta gtacccgttt acatttcgta tcagcccgac gaattccgtt
      661 tcaggggcct atttcgtagc aaagcccacc cagtgccgcg cggtgcaatg cgtggtcgtt
      721 gctgacaacc aaggcgaata gtccaccgtc aaggatcttg cgcttctcgt ccgcaaagcg
      781 ataaagcggc tggggcagca gccgcaactg cgttgttgct ccattgcgag agcaggaggc
      841 tgaaaatctc cgagcgaagt cgcgcatttg ccaacgcgct tagggttacg gtgctgtcgc
      901 gctgaaccgt tgcactgcga acgtgccttt ctatactagg gcagcggact tgcggccgag
      961 aaagcgtgaa cttgccttcc acgccattat cacgtcgttt ggaatcccac aatgtgtgac
     1021 aacctgattt ttcgtctgtc ggcgggcgtg gctgtggcag ttggcgccgt cttattggga
     1081 ccatccgcgg cacaggcaca ggtggctcgc gaaccgaagg cgtccggcag cgcggcattc
     1141 tcggaagcgg ttgcacgggc caaggagagc agccgtccgc tggtcgtgtt cggcgttgcg
     1201 gagggctgca cgcgatgcgc ggctctgaaa caagggttgt ccacgcagtc ggagctgaag
     1261 ttgctgatga cgcaatacgt ctccgtcgag gttccgttcg ggggacgcga gttcgccgcc
     1321 atctttgagg acatcgttcg ccaggatgcc aagttccgcc aggcgatcgg tgcaccgtcg
     1381 gtcttcatat tcacagccaa gggggacgcc gtttatgcag gccctaataa cgcgaatgga
     1441 atgttgcccg gcgacgaatt caaaaaactg ttgatcgcag ggatcgagaa taacggtgga
     1501 ttgcgcggcg ccgtcaagtc ctcgcctgcg aacaacctgg ccaccgacct cgcgaaagct
     1561 cgtaagcaat ggggcgagaa ccagccggtc gccgcagccg cgaccatttc aaagtacgtc
     1621 tcatcggaca aggcagacgg cggcgaggtt gcagagctgg tcaagctgac ggggttgaat
     1681 gtcgcccagt caaagatgga agagcagctc gattccttgg tggagcaggt ttccgaaaaa
     1741 gggcggtctc tcctccaggc ggcggtgacc ttggcaagca ccggcgacgc gacgcgctcg
     1801 gccgttcagc ttgccgagct cgaccgcgtc ttcggggctt acccgtcgtt tgcgggcaag
     1861 ttcgacgccg cttggaaaga attactggcc aaggcgagtg ttcctaatct caaggagcag
     1921 gccgaagcga ttgacaaggc tcgccaggcg gaaagcgcgg cgggcccaga ccaggccatc
     1981 gcagcctacg agcgcgtctc cgcaacctat ccaggcacca aggccgccga gctttcccag
     2041 cttcggatta cccaattgcg gacgccaaag tcggccgtcc cacgactctg gaaaagcaag
     2101 tccggccaat tctccgtcac cgccacgctg gttgcctttg atggcaaatc ggcccaactg
     2161 cgcaccgcag aaggcaaaac aatcaccatc gccctcgagg ccctgtcggc tgaggaccag
     2221 cagttcctaa tgtcggtcaa ggcgccgaac tgaacttgtg cgaggcgaag gggcgacggg
     2281 ggcttgtgga gctaccggcg gcggagtcgg agcttgggcg ggcgatggcg ctggcgccaa
     2341 gtcgatcgcc tacaacctgg tcgcgaattg ccggtccggc tgccattgcc tgggcacagg
     2401 caaatcggga cggatcacgg cgggtggaag ctgccgctac ccggaatcgg cgcgaaattt
     2461 cgcggatcgc gaggcgccgg gcgcgaataa tagatagggg gcgattgggc gttccccaac
     2521 gggagcctca gccatgaaag cgaacaagat cgtgtttccg acggacctgt cgaagaccag
     2581 caccgcggcc ttggggtggg cgacggtgct ggcccgcgat accggcgcga cgctggtgat
     2641 cgtccacgtc gaggagccgc cggtggccta cggcggcggt gagttgtatt acggcatgga
     2701 cgtgggcgac cgcgagaagc tgcagcaggc gctggccggc gtgcggcccg ccgatccggg
     2761 cgtgaagttc gtccacaagc tgctggtcgg caatccggcg cgggccatcg tccagaccgc
     2821 caaggacgag caggccgacc tgatcgtcat ggggacgcac ggccgcacgg gcctcttgcg
     2881 agtgctgatg ggcagcgtcg ccgagatggt cgtccgcgag gcccagtgcc ccgtgctgac
     2941 ggtcaggcag cccgccaagt aggcctgcta cggtgcggcg agcagcgcgc ggcgcgtccg
     3001 cgagatgctg acgaacatgt gaatcagcgg cacgacggca gcaatcagca gcaggatggc
     3061 gagcgggccg atttccagcg aagcgcggcc ggcggcggcg aaatacagcc agccggccgc
     3121 gtgcatgatc gcgctgggaa ccaggctcag tatcaggaac agcagctggc tggtgcgcca
     3181 gctcgcttcg gcctggtcga gccgaccgag ccagcagaac gtccgcatcg cggcgcagaa
     3241 caaaaagatg cagacgaggc agaccaggct aaacagcgtg ctcacaacgc caaagagggg
     3301 cggccagggc agcacgaggt ggtccagcac ggcctggccc agcgccagca gcgaaacgca
     3361 gaaacaaagc gacatcgtcc ggcgataggc cggctcatcc cagatcgccg acagctgata
     3421 cacgccccag gcgatcagca gcatgcccac cgcgtcgttg agaacgtcga agcggatgcc
     3481 gctgccattg gtcgtatagc tcagccagaa atccagcacc gccaggagcg tgccccagaa
     3541 gattttgccc agggcccgct cggcggctgc cgcggcctgc ggcgaaagcg tgggtctttg
     3601 ttgtggccca aacatgccgg tagattcgct gccggctcgg attccttgcc ggccgcgagc
     3661 ggatccgtca cgacgccggc ttcacggcga ccggtttcgc cggcagcacc tcggccagca
     3721 ggcaccgctg gtcctggtag atgatccgca cgcctccctg gccgctgcgg tcctccatga
     3781 ggatggcctt cagcaggtcg gggtagcgct tcttctgaac gagcacatat tgcatgtggt
     3841 ggccgcggag gaggtgcagc cagacctcgt caccacgaaa gacggcttcg tagtcgcgcc
     3901 aagtgtcgag ctcggtgagg tgcacgtgcg aatgcaccag cggcttcagc cggccgccgc
     3961 tcttccagac caggaaatcg ctccagtcca tcggcgttgc gatgttgccg gacaggtcgc
     4021 ggcggaccgc ttcgtcggcg acgtagatcg gcgtgtcggt cacggtgatc gccccttcgc
     4081 cgcggccgta gccggcgacg atggcaaacg tgggcggcgc gacgagcagc gtcatgaaga
     4141 caaagcccat cgccagcacg gtccgcatcg ccgtcggttc gtcgccggcc tgggcgggct
     4201 cgccggctct gtcggccagg cgctttcgcc aggcggcggc cgcgtgcgga atgcagacga
     4261 ccggccagac gatggcccac caggcgagca tgcgaatggc cggcagcgtc gccaggccga
     4321 acagcagcag cagaccgatt tcgtagagct cccacttttt cgcgctgcag cgagccagca
     4381 gcgccgccag accgaccgag acgatcatca gaccgccggt caggctgagc ggcgacagcg
     4441 gccgccattc gctgatcgag gagagcgccg cgtgttcatt gaacaatagg acgcgggcca
     4501 gcaactgcgg cccgtgcggg ttgaggcaag ccgcggccag caccagcagg agcgcgcccc
     4561 acacgacgta cagccgccgg tcgcggagca gttttgcggc gtcgccgccc gcctcttgcc
     4621 aggcggtcca gctaacgctg gccgcataaa ttccgagcac cgcgatgccc atcaggatgg
     4681 agccgtgcag gttcacccac agggccgcca ccaccggcag ccagaggagc gggtgcgttc
     4741 ggcgcggcat ttcggcacac gccagcagga ccagggccag gccgagctgg ccgaagagct
     4801 gcgggcggat cgtcccgacg atcggcaggt cgagcaggaa catcgccagc cccgccgccc
     4861 aggcccagag ggcgggcgct ccgcggcgga ccgcagcgag catgacgatg ccggcggtga
     4921 gtgtgacgag cagggcgtgt cccagggcga gcccttcgtt gccgaagtgg gtctgcactt
     4981 cgtagccggc gagctgcgac agccaggccg cctgcagcac gggcgtggcc gcgggcgcgg
     5041 tggcaaaggg gtccgtcgcc ggcagtgctt gatgctcggc catccagcgg ccgaagttga
     5101 gatggcccca caggtcggtg tgattcagcc ggttgaacgc ggtcagcatg aagatgcccg
     5161 acagcgtgca aacggcgatc aaccgggcga agctgaggct cggaaaatag ggactcgtca
     5221 ggttgtcgcg cgtgatgcag gccggcagct cggttggcgt gctcatggag ttgtcactgg
     5281 tcattggtca cttgtcactg gtcgctgcga cctgatggct cgctgttgca gtgcccttct
     5341 tccaagcgtc gatcggttcg gtgctccgcc acaggcagag ggcgcgggcc gcgtgcgtgt
     5401 aggggccgta ggcttccagc aggtctttct ggtccatttc gacgagcgtc cgggccagcc
     5461 actgcgcggc gcggatcacg gtctcgcgcg gcggctgcac ctcttcgggc gcgagggcca
     5521 gccactccag gtgatggccg gtcacgagca gcttgtcgtg cagcgtggcc gggttgtcct
     5581 tgcccggttg cgacgccgcc gctccgcgcg gccactgccg cgtccaatag ccttccgcgg
     5641 tctgggcccg cacgaggacg tcgctggtcc acttcagggc ggtcagcacc ttgagttttg
     5701 tcttgggctg cagcgcccga tattcgtcgt cggcccggtt gagcacgacc agcgcctcca
     5761 ggcgatgcag accgttgcag ggcccgatgc ccggcggatt cttcaggatt tgatcgacca
     5821 gctgatcgac gttgatcttc tcgccatact tgttgcgcca gccgggatgc ggaaagacgt
     5881 agcgggcgta ggcgatcgtg gtccactcgt attcgagctg ttcgggatga gcgcggagca
     5941 gggcagtcgt cagcaggtcg ccgacgcggg cctgtccatc gcgcagaacc agcggcgcgc
     6001 ccgccggcac gcccgtctcg gccagcgtcg cgaccaggtc gtcggtgtga aagctggaag
     6061 tctcgcgatc ggtgacgcgg tcgtcgtacg agcggacctc gatgccatcc cggccgcgaa
     6121 aaaagagcgg cggcgcctgc tcgccggccc agcggcgaaa cgccctgtcg ctcagaaaat
     6181 agtctcgcag ctggcggccg ctggggatct tcgcatcgcc gaagtccgcg ttaggccccc
     6241 acagccgcag ggcgtggacg aagttgttcg tactgaccgg atcggccggg ggcaatacac
     6301 gatcgagcac ggccgccagc tgggcatcgg tcacgacgcg cgggtcgttg attcgcggcg
     6361 cgatgaccgt gggcatattt cgcggcagca cgacggccgc cggctgcgga ccgacagccg
     6421 ccattcgata gacggccagt gcaccggcga caatcaacac gggcaaaacg aagcggctaa
     6481 tgcgagcgag catgagcaag tctatgtcaa attgtttaac ccggagccgg aggcgacggc
     6541 ggctacttta cagcagcccg aactttttca tcttgttgta gagcgtgacc cggctgatcc
     6601 cgagttccgc ggcggtttcc ttgcggcgga agttgttccg cgccaggcag tcctgaatga
     6661 tccgccgctc cagcacatcg acgcgggctt cgagcgatga ggccaggcgg gcagccgtcg
     6721 cggtcgcgcc ggccgcatgc gcctcggcct ggcctccaat gcgggccgct gccagcggct
     6781 ggggaatgtg caacgcaggc tgcggcatcg ccatccgcgg cggctgcggt cccaaggggc
     6841 cgacgcggac gcgcggcggc agggcggcga gcgtgtgatc gaatagcggg tcttcgacga
     6901 tggccggcgg cacgcggtcc accgaggccg cgggaagcga ttcgaccaga ccattggcgg
     6961 cgtcgcggat cgagctgggc aggtcctgca ccgtgagcac gccgcgatgg caatacagga
     7021 cggcccgccg gacgacgttt tccatctcgc ggacgttgcc gggccagtcg taggctttga
     7081 gcgtttccag gaacgccggc tcgatcgacc gcagctggat gccgtgcgtc cggccgtgct
     7141 cgacggcgaa cttgcgggcc aggtattcga tgtcccaggg ccgctgccgc aagggcagca
     7201 ggcggaagtt caggatgttg aggcgataat agaggtcggt gcgaaaacgg ccggcccgga
     7261 ccagctcttc gaggttgtag ttgctggcga cgatcagccg ggcctgcgag tgctgcgtct
     7321 cgttgctgcc gacctgctcg tattcgccgg tctcgatgac gcggagcagc ttcgcctgct
     7381 gctccagcgg cagcacgtcg atttcatcga ggagcagagt gccgctgccg gccgcggcga
     7441 acttgccttc gcgattgtgg tcggccccgg tgaacgcgcc cttgacgttg ccgaacagct
     7501 cgctctcgat caggtcgggc ggcagcgcgc cgcaggccac cgtgcagaag cggtcttcgc
     7561 ggcggtccga cagctcgtgg atcaagcggg ccagaaaagt cttgccgctg ccggtctcgc
     7621 cgatcaagag caccgtgacg ttgtggcggg ccgcgacctt gatctcgtcc atcacgtcga
     7681 acatctcggg cgtgaaggtg accagcgaac ggctgcggcc gtgcagctcc ttgtgcggca
     7741 tctggctcca gaacccggac agctcgccct cgacatcctg cttttccgag agcaggcggg
     7801 tcagccggcc gtaatcgagc gggatatcca ggcagtcgtc gatggccttc tcggccagct
     7861 tcttcagccg cggggggcag ttctcgtcca tcaggccgta aatcggggcc tggtcgatgg
     7921 cttcgtcgag ctcggcgatg aagcgggccg gcgagtagcc gttgagcgtg ttgcgctcca
     7981 ggtgcaccag gacggcgctc aaccgctgat cgccgacgcg ctgcggcagg gcgtcgagcg
     8041 tttcgcaggg gacgatcgca tgctggccgg ccagctgctg gccgagctcg aacaggagtt
     8101 tttgatcgcg ggtaaagacg aggatgctgg gattcatgcg gttaccaagg agcgatgctg
     8161 gcagagggcg atgcgagtgc ctcgcccgac ggaaaactca gcccacattt ctcacgctac
     8221 ttggaccgga aaactgaact agcgccaaat tgcgttaacg aaagggaatc atgctgaaac
     8281 gcggcgaaat tgcgggctgt gccggattcg ccggcgatgc cgcccgtgcg ctcgcgctgg
     8341 ctacagtctg gagaagctgg cgaagcgcta ccgcctgtgc tggcgttgcc gatgatgcct
     8401 gagaaactcg aaactcccgg cggttgggtc gatgcagagt agtggaacgg gttgtaacgg
     8461 cgcgccggat actgcgaggg ccgcctaagg ccagaggtcc gcggagaaac tgaaccatga
     8521 atgcgattct gctgacggtc ttggcgctgg gcggggccga cgccgcgatt tttggtggcg
     8581 gcggtggagg cggcggcgaa tacatcgacg gtggttgcgg cgacggcggc gagtgcgacg
     8641 gcggcggctg gcacaacaag gccggccacg gcggctggct cgggccaatg ccgcagtcct
     8701 gctactggcc gtcgttcggc tgctatccca gcacgccgtg gatgcaccgc tatccggcct
     8761 tccacggcac ttactaccgc aaggcctaca actaccggaa ttacttcgac tatccctggc
     8821 acgccgggct gcacgagccg acttcgcact tctcgtatca cgtgccgggc gaagcgccgg
     8881 cagcgggcgg cggcggtgcg gtgccgcggc cgactcccga cgcggcctat gcgccgcggg
     8941 gcggcctgcg aacggcgacg gccgagggcc ggatcagcga cggaatctcc gtcacaccgt
     9001 acaaggttca gcggtaggcc gctggaccag ataacaaagt ccagcggtaa ggccgctgga
     9061 ctccagcgaa tcccatccgt gtagtcggac gtcgggcgcg tgccggcgtc cgattttttg
     9121 ctagctcgcc tagcgggcgg gccgcggcgt aagcacgggg cgcggctcgg gttggggttc
     9181 gggctgcggc tgagccatta gccgctgcat gccgtccagc ttgaccttgg cccgcatcac
     9241 cttgatttcc gcggctttca actcgaactg atgcttggcc agctcggcct ggctgatggc
     9301 gctggttcca tgcttgcgaa gcgcctcggc tcgctcggcg tcctgctggg ccgcctgcaa
     9361 gccgatctcc gcttcctgca ggtcaagctt gagcagctgc aattgcgttt cgcggtcggc
     9421 gagagtcagc cgggcggcct cgtccacatt aggccggtgg agcgggttga gagtggaatt
     9481 tgggttgcga ctggacggcg ctaattcgtt cggcagctgt ccctgctgtc cctgctgccc
     9541 ctgctgtccc tgctgccctt gttgcccttg ttgcccttgt tgaccttgtt gaccttgttg
     9601 accttgttga cctagttgtc gttgaggcgc gggagggtag ggcgagtttg actggccggg
     9661 ccgatcgagc cgggcgatga tcgttttgat cgcgtcccag tgcctcgatt gcgccgagac
     9721 gatcagcgaa ttcgaggcct catcgacgga ccacttgatg ctcgccactt gtgcgtcccc
     9781 gcccagcagt ctgacttcgt cgagcaagag cttgagcgcg tcggccacat ccgcggcgct
     9841 ggagttcctg aggcgaatga tggtgttcgt ctggaccggc gccagctggt ccagttgggc
     9901 cttcaaatcc gcgagctgct tccggagcgc ctcgctttgt tcccgctggg cctgggcttc
     9961 cgcggtccgc tccgcaagcc gccgctcgag ctgccgtacc aaacgccgca agtcttccgc
    10021 ctgcgcggcg gccggcttgt cggcctgctc ttcgcgctgc gcgagttgca cctcgacggc
    10081 caccagcagc ggtttcagct cgagcttttg cggccggctg gccacgatca ccgtttgccc
    10141 gagcggaacg acggccttgg tggcgagctt ctgctgcgtg gcaaccggcg agccatcggg
    10201 gcccagtttg agatcgactc ttgctgcgtc cagtgcgagg acgacctgtt gcttggagtc
    10261 ttgaagggtg agcttgggcg tcagctgaat cgtcaagcgg ctcggtttgg attcggttgc
    10321 cgccccggtg tccgcggtgc tttcatcggg agattgaggc ggcgacttgc cgccgatttc
    10381 caccagtgcg agctggccgt ccagcgtgac aatctgcggg cggctgagaa tgaccgtgcc
    10441 gggccgcgcc ttcagctcgg cgagccgctg cttccattcc tcgggcgact tcaaggtgat
    10501 ctcggtctcc ggatcgggct gcggacgggc ctcagtgggc agcacagcgg tcagctcggc
    10561 ccgctcgacc tcgagcacca cgcattggag caggatcgcc gtaggtgtct ggtccggcga
    10621 tccgctgggc gcggcttcgc tcgccggcgg ctgggcttct tgcccgtcgg cggtcggcgg
    10681 gcgcagcgtc accagcgaca ggccgccgat cagcaggagt gtggcggcga ccagcaggaa
    10741 ttgccagcgg cgactgccgg acgaaagcgg gcggcgcgaa tcgagcaaca tggtcactct
    10801 ccgggaaagg gtgtgaggag attcccacaa tgacaaggcg gccaggccgg ccggggccga
    10861 gctggcggcc ttggcccaag ccacgagcgc ctcggcatat tcgacgggct catcgcccgc
    10921 cgcggctgca tcggccagca gttccaggtc cagacgcacg ctgcgccgca accaccagaa
    10981 cagcggctgc caggcgagca gcgggacgag ccagcgttcc agcgccagca gccacaggtc
    11041 gccgtgccgg atatgcgccc attcgtgcgc cagcgccgcc cgcacgatcg gccgccgagc
    11101 ctcgtccacc gcctccgccg gcagcagaat ccgcggcgag agcgcaccga cagcggctgc
    11161 cgaatgcaac tgcgggctga tgcgcacctg cgggccgcgg cgcgggccgg taactttggt
    11221 caattcgtcg atcacccagc gcggcgcgga caacgagcga cgaaccagca accaggtttg
    11281 ccagagcccc agcccgatcc aagccgcggc gaaagaccca atcgccagcc agccgccgag
    11341 aatcagctgc tcccagcgaa gatgcagcgc agacggcggc tgaatcgctg ttgactcggg
    11401 cgtcacgacc tccagggcgg gcaattcggt gacgatcgcg gggggtgatt cgacttgcgt
    11461 tgccggttgc tgatcgatca gcgcgacaac ctcggcctgc gtcagcgagc caagggccag
    11521 gcgcggccac tgcggcagcg cgatcgcaat tgccaataac accaggccga gccaggttcc
    11581 ccaggccagc gccacgcgct gcgccggatc gcgaaagcac ccgcgaagcg ctgcggcggc
    11641 ggcgagcacc gcggtggcca aaagcgcata gtccagcagc caggcggtca gggtgacgct
    11701 ccagttcatg gcgaatcctc cttccgcttg cggcgggacg gtttgtccgg cctggcggcg
    11761 gcgattagct gttccaggcg gcgcagctct tcgggcgaaa gccgccgggc ctccagcgca
    11821 tgcagcacgt attcgtcgag tgagccgtcg aagacgcgct cgagaaaccc gcgggccagc
    11881 cgctgaaacg tgcggtcgcg gtcggccttg gcgaagtacg aatagacctt gcccgtggcg
    11941 gtgtggccga cgagcccctt ctgctccata acttgcagca ggctcaagac ggtcgtatag
    12001 gccaggtcgt cgccccgctc gttgatggcc gcggtaatct cgcggaccgt cgcctggccc
    12061 aactgccaga gcactttcag ggcctgcagc tcgcgttcgg taggcgccac gtcagccacc
    12121 ggactactcc cctgctcgaa aagcgcggca atctactgga cgaccagtaa ttcagccgga
    12181 agtattactg gtcggccagt agatgtcaag agcgcaagcg accgcgaagg ttctcggcca
    12241 gcgcaatcag cgtttccacg ggcaattcct cggcccgggc ctcgggagcg atcccctccg
    12301 cggcgagcag tgcgtcaatg gccggcctgc ccaactgctc gcggaagcag ccggcgagga
    12361 cgccccgcag cagcttgcgg cggtgacaaa agacatcgcg aatcagcgtg tggaagaact
    12421 cgacgtcgct gaggcgggcc cgtttggcgg cgctcggtac gatgtgcacg atcgccgact
    12481 gcaccttggg ccgcggccag aagacttgcg gcggcatgac gcggacgatc gcgatctcgc
    12541 acagcgcctg catccagatg ctcagggcgc tatagtcctt cgtgcccggc gcggcgatca
    12601 tccggtcggc cagctccttc tgaatcgtcg ccgtcagcga gaccggcagc ggatcggccg
    12661 tgagcaggtt tgcgaggatc ggcgtggcca cgttgtaggg caggttcgcc gccagcttga
    12721 agcggctgcc gggatgccgg gccaggtgct cgcggaccgc ggccagcacg gcggggtgca
    12781 tggtgctctt gttctgaagc gcgtcggtgg atagcagcgt cacattcgtt gcgcccgcct
    12841 tggccagttc ctccgacgcc agctgctgca aacgcgcgtc gatctccacg ctgacgacgg
    12901 cggcggcttc ttgagccaag cgcgtcgtaa gtgcgcccaa gccggtgcca acctccagaa
    12961 caacatcctg cgggcccacg tcggccgtcc gtaccagcag gtcgagcagg ttgaggtcga
    13021 tcagaaaatt ctgaccgcgc cgcgtctgcg gccggatgcc ggcctcctcg aagcggctca
    13081 tcaaatacga ctgcgtctgc cgcggcgaag tcggacgaga tgtttgcccg agcgatacgt
    13141 cgtccctcat atgccagtca gcgcgttcca ctgctcccaa cgatttgtgg cctcaaacaa
    13201 aagttgttga tggagccttt cggcgtagtc attgatttcc cgtcgttgcc agtactcgac
    13261 atatcgccgt aacgagtcgt agttttccgg agacgtcagc tccaaccaaa ttgcaagctc
    13321 atccgctccg ctcggcacga acacatcctc tgttaggaca tcaacggcgc tcacccccgg
    13381 cttgcaggct cgtcgccatt tggcctctga cagcgcaccg aacaggcgat ggattcgttc
    13441 cagttcgtcg tccatcagga attgcaattg aagaaactga gactatggac tatcagcttg
    13501 cgcaaagtca gagtacgagc ggtccggtag tcgctcacca tcgacggcga gttggcgctg
    13561 cacgtacttg gccagcaaat cggtctcgat gttcacgagg acgccgactc gcaagtcgcc
    13621 gagcgtcgta ttctgaagcg tgtgcggaat gagggcgacg ctgaagcggc ccttgccgac
    13681 gctcaccagc gtcaggctca cgccgtcgac tgccaccgag cccttctcgg ccatctgccg
    13741 ggccagggcg gccggcacgc gaaaggtgac ggtcgcccac tcgccttcgt cgcggaattt
    13801 gatcacgctc cccacgccgt cgatgtgtcc cgtgaccagg tgcccgccca gccgatcgcc
    13861 caaccgcagc gaccgctcga gattcacgcc gtcgccagcc gtcagccgtg agaaggtcgt
    13921 gc
//
LOCUS       DASRJB010000838         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_805937, whole genome shotgun sequence.
ACCESSION   DASRJB010000838 DASRJB010000000
VERSION     DASRJB010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805937"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="ald"
                     /locus_tag="VFB80_22060"
     CDS             complement(<1..1087)
                     /gene="ald"
                     /locus_tag="VFB80_22060"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZN36537.1"
                     /translation="MLIGVPREIKLDEYRVAMLPVGVEELTRAGHQVLIERSAGIGSG
                     LPDSEYAAEGAEIVASAAEIYKRADLIVKVKEPLPPEFLLIRAGQTLFTYFHFAASRE
                     LTEAMLASRATCLAYETLRDAQGRLPLLTPMSEVAGRMSIQEGAKYLERPQMGRGILL
                     AGVPGVAPAHITILGGGVVGANAAKIAAGFQADIAILDVNIDRLRYLDDIMPANVNCL
                     YSDRHTIRTQLKLADLVIGAVLIPGAKAPRLISRQDLKLMKPGSVIIDVAIDQGGCIE
                     TSRPTTHSDPTYLEEGVLHYCVTNMPGAVGRTSTFALCNATLPWVLTLAKSGIEAALR
                     QSPPLASAANIVAGRITNQAVAETFGLK"
     gene            complement(1170..2597)
                     /gene="der"
                     /locus_tag="VFB80_22065"
     CDS             complement(1170..2597)
                     /gene="der"
                     /locus_tag="VFB80_22065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZN36538.1"
                     /translation="MSVPQVVIVGRPNVGKSSLLNWLCGRRISIVDDQAGVTRDRVTH
                     LMEHNRRFFELVDTGGIGIEDCDNLTKEVEQQIDAAIETADIILFVVDTRTGMMALDS
                     EVAKRLRYIDKPIVVVANKTDHEGLDADADDFYRLGRGKLMKTSTQQNRGKEELLELI
                     HSKLPPNIMLEPLPEEPVMKAAIVGRRNTGKSTFVNTLAKADRMIVSEVPGTTRDSVD
                     VQFELDGKQFVAIDTAGVRRHKSIRTDIDFYSFHRAQRSIRRADVVLLFFDCTQRLSK
                     VDKQLCKYIADQYKPCIFVVNKWDLLYGQMPTERWVDYLRDTFPTMWHVPIAFITGQT
                     GKNVKALLNHAQNLFKQCTDRVHTSHLNRLVRAAVERNPPPLHGILRPKIFYATQVGV
                     QPPTIVLICNSPKAFPASYRRYLLSILRDNLDFGEVPIKMFLQKRRRNDERDDVGAGE
                     DTPAEAALIEEEQDSELEYGEVHDD"
     gene            3007..3672
                     /locus_tag="VFB80_22070"
     CDS             3007..3672
                     /locus_tag="VFB80_22070"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN36539.1"
                     /translation="MPRAKSNATLSPTLHSLIGQPYPSDLPDSAADRQRLLDELNAQV
                     RPCLLCRELASGRKQTVFGVGNSKPRVVFFGEAPGADEDRQGEPFVGRAGQLLTKIIE
                     ACGWQRSDVYIMNVLKCRPPDNRNPLPPEVDNCRPFFERQLEILRPEYIVCVGSVPSQ
                     ALLETTESVGKLRGRIHRYRGSRVLVTYHPSYLLRNPAAKKYVWEDMQLLLKEMGLPV
                     PKR"
     gene            4083..4769
                     /locus_tag="VFB80_22075"
     CDS             4083..4769
                     /locus_tag="VFB80_22075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014701238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN36540.1"
                     /translation="MSPRSFVRGIFLGMALACAAARGQDNPAQLFKTLDDQASYAIGL
                     DVGRSLLADAPELNPDLVARGVIDAMRKARPLLSDAHLHEVMDLFAAHKAGPAAEKTL
                     KEGQAFLAANRAKPGVQTTESGLQYIVLKAGAGKSPQPADIVSVHYQGTLIDGKVFDS
                     SIERKKPAEFPVNRIIPGWAEALLKMKVGDKWRLFLPARLAYGPQGSPDSKIPPFSVL
                     VFEVELLEVK"
BASE COUNT          937 a         1711 c         1692 g          994 t
ORIGIN      
        1 atttaagccc aaaggtctcg gcaacggcct ggttggttat ccggccggcg acgatgttgg
       61 cagcggaggc cagcggcgga gattgccgca gggccgcctc gatgccgctc ttggcgagcg
      121 tcagcaccca gggcagggtg gcgttacaca gggcgaacgt gctggtgcgc ccgactgcgc
      181 cgggcatgtt ggtcacgcag taatgcagca cgccctcttc cagatacgtc gggtcgctgt
      241 gcgtcgtcgg ccggctggtc tcgatgcagc ctccctggtc gatggccacg tcgatgatca
      301 cgctgcccgg cttcatcagc ttgaggtcct gccgcgagat cagccgcggg gccttcgcgc
      361 cgggaatgag cacggccccg atcacgaggt cggccagctt gagctgcgtg cgaatcgtgt
      421 ggcggtcgct gtagaggcag ttgacgttgg ccggcatgat gtcgtcgagg tatcgcagcc
      481 ggtcgatgtt gacgtcgagg atggcgatgt cggcctggaa gccggcggcg attttcgcgg
      541 cgttcgcgcc gacgaccccg ccgcccagaa tcgtgatgtg ggccggggcg acgccgggca
      601 cgccggccag caggatgccg cggcccatct ggggccgctc caggtacttg gccccttcct
      661 gaatgctcat ccgcccggcg acttcgctca tcggcgtcaa aagcggcaag cggccctggg
      721 cgtcgcgcag ggtttcatag gccaggcaag tcgcgcggct agccagcatc gcctcggtca
      781 gctcgcggct ggcggcgaag tgaaagtacg tgaacagcgt ctgccccgcg cggatgagca
      841 aaaactccgg cggcagcggc tctttcacct tgacgatcaa atccgcccgc ttgtagatct
      901 cggccgcgct ggcgacgatt tccgctccct cggcggcata ttccgagtcc ggaagcccgc
      961 tgccgatgcc ggcgctgcgt tcgatcagca cctggtgccc ggcccgcgtc aattcctcga
     1021 cgccgacggg cagcatggcg acgcggtatt cgtcgagctt gatctcgcgg ggcacgccga
     1081 tgagcatgac cgactccgtt tgcgggcaat ccaagccgat gcgaccagct tagcgggtgc
     1141 ggctgatgcc gaacatgcgg cgcgagcggc tagtcgtcgt ggacttcgcc gtactccagc
     1201 tccgaatcct gttcttcctc gataagcgcc gcctcggcgg gcgtgtcctc gccggcgccg
     1261 acgtcgtcgc gctcgtcatt gcggcgccgt ttctgaagga acatcttgat cggcacttcg
     1321 ccgaaatcga gattgtcgcg caggatgctg agcaggtatc gccggtacga ggccggaaac
     1381 gccttgggcg aattgcagat cagcacgatc gtgggcggct gcacaccgac ctgcgttgca
     1441 tagaaaatct tcggccgcaa aatgccgtgc aatgggggcg ggttcctttc caccgccgcc
     1501 cgcaccaggc ggttcaggtg cgacgtgtgc actcgatcgg tgcactgctt gaacaggttc
     1561 tgggcgtggt tcagcagggc cttcacgttc ttgccggtct ggccggtgat gaaggcgatc
     1621 ggcacgtgcc acatggtggg aaacgtgtcg cgcagataat cgacccaccg ctcggtcggc
     1681 atctggccgt acagcaggtc ccacttgttg acaacgaaga tgcagggctt gtactggtcc
     1741 gcgatgtact tgcaaagctg cttgtccacc ttcgagagcc gctgcgtgca gtcgaagaac
     1801 agcagcacga cgtcggcccg gcggatgctc cgctgggccc ggtgaaagct gtagaaatcg
     1861 atgtcggtgc ggatgctctt gtgccgccgc acgccggcgg tgtcgatcgc cacgaactgt
     1921 ttgccgtcca attcgaactg cacatcgacg ctgtcccgcg tcgtccccgg cacctcgctg
     1981 acgatcatcc ggtcggcctt ggccagcgtg ttgacgaacg tgcttttgcc cgtgtttcgc
     2041 cggcccacga tcgccgcctt cattaccggc tcttcgggca gcggctcgag catgatgttc
     2101 ggcggcagct tgctgtggat cagctccagc agctcctcct tgccgcggtt ctgctgcgtg
     2161 ctggtcttca tcagcttgcc gcggcccagg cggtaaaagt cgtcggcgtc cgcatcgagc
     2221 ccttcgtggt ccgtcttgtt tgccaccacg acgatcggct tgtcgatata ccgcagccgc
     2281 ttggcgacct cgctgtccag ggccatcatg cccgtgcggg tatcgaccac gaacaggatg
     2341 atgtcggcgg tttcaatcgc cgcgtcgatc tgctgctcga cttcttttgt taaattgtcg
     2401 caatcttcga tgccaatgcc ccccgtatcg acgagctcga agaaccggcg attgtgctcc
     2461 atcaggtgcg tgacgcggtc gcgggtgacg cccgcctggt catcgacgat ggaaatccgc
     2521 cgcccgcaca gccaattgag caggctcgac ttccccacgt tcgggcggcc gacaatgact
     2581 acctgcggaa cggacatggt gcgggtggaa tcgcggcggg cgagaaactc ggtcgggtgg
     2641 gaacgccgtt gcgcggcgaa acgggcaaag aactgtatga tagtctggcg acggcgcttc
     2701 gcgaaaggtt gcggcttcct ggcggggctg ccaattcgcg ggcggcggcc gcaaaatcca
     2761 ggatattaaa cgccgtccgc cccggtttgg ttcggagaaa tccacttgga agagcgcgat
     2821 cgtctccttg tcgctcgcca cgtgctgacc cagcggctgc aagcgctgga attggccgga
     2881 gttcagcaga tcggccgcgt gccgccgcca gccccgggga ttctcacaag cccgccggct
     2941 gctgccagcg tgcggaatca agtggtcgca acgccggcgc cgcaatcact tttgaccgag
     3001 ccagcgatgc ctcgcgccaa gtcgaatgct actctttcac ccactttgca ctcgctcatc
     3061 ggtcagccgt atcccagcga tctgcccgat tcggctgccg accggcagcg gctgctggac
     3121 gaattgaatg cccaggtccg cccctgtctg ctctgccgcg aattggcgag cggccgcaag
     3181 caaacggtat tcggcgtcgg caattccaag ccgcgagtcg tcttcttcgg cgaggccccg
     3241 ggggccgacg aggaccggca gggcgagccc ttcgtgggcc gtgccgggca gctgctgacg
     3301 aagatcatcg aagcctgcgg ctggcaacgg tcggacgttt atatcatgaa cgtgctcaag
     3361 tgccggccgc cggacaatcg caatccgctg cctccggaag tcgataactg ccggccgttc
     3421 ttcgagcggc agctggaaat cttgcggccg gaatacatcg tatgcgttgg ctccgttccc
     3481 tcgcaggccc tgctcgaaac gactgagtcc gtcggcaagc tccgcggccg gatccaccgc
     3541 taccgcggca gccgcgtgct cgtcacctat cacccgtcgt acctcctgag gaatcctgcg
     3601 gccaagaagt acgtctggga ggacatgcag ctgctgctca aggaaatggg cctgccggtg
     3661 ccgaaaaggt aaactcagaa tgcagaattc cagagacaac cctgccttca ttctgacttc
     3721 tgaattcaaa aaacagccgc ccgcctgatc gcgagcgacc cggcgggcgg cggaagtgtg
     3781 cagaaaatgt ggaagaacga ctggcctgag cccagcgcaa gcgagcgctc ggctcagtgg
     3841 tacaggcccg gtggatcaag cattttgccc ctcctttggc cctcgctaga agagggcgtt
     3901 gctaccagat gaaatccgtg tggaggatgc atcccacaac tctattatcc gctcggtccg
     3961 caaattcgca actaattccg gccaggattt gccaggcact ttcaccacac acgccgattt
     4021 ttggcgagcc ctagcggtca gccaaactct gcggctgtaa cccgcccagc ttggaatcaa
     4081 ccatgtcgcc gcgctcattc gtcaggggca ttttcctcgg tatggcgctg gcctgcgccg
     4141 ccgccagagg gcaagacaat cccgcgcagc tgttcaagac gctcgacgat caggcgtcgt
     4201 acgcgatcgg cttggatgtc gggcggagcc tgctagccga cgcgccggaa ctcaatcccg
     4261 acctggtggc ccgcggcgtc atcgacgcga tgcgcaaggc caggccgctg ctctccgacg
     4321 cgcatttgca cgaagtgatg gacttgtttg cggcccacaa agccggacca gccgccgaga
     4381 agacgctcaa ggaagggcag gccttcctgg cggccaatcg ggccaaaccc ggagtgcaga
     4441 cgaccgagtc cggcctgcaa tacatcgtcc tcaaggcggg cgccggcaag tcgccgcagc
     4501 ccgccgacat cgtctccgtc cactatcagg gcacgctcat cgacggcaaa gtcttcgaca
     4561 gcagcatcga gcgcaagaag cccgcggagt ttcccgtcaa tcggatcatc cccggctggg
     4621 ccgaagcgct tctcaagatg aaagtcggcg acaaatggcg gctctttctc cccgcccgcc
     4681 tcgcctacgg cccacaaggc agccctgaca gcaagattcc gccgttctcg gtgctggtgt
     4741 tcgaggtaga gctgctcgag gtgaagtgag ccagggtggc tgtggtcggt gcagccggcc
     4801 acagcctggt cggggatgta cgcgcttccc gcggcaaccc aagcgggcga aatcgatttt
     4861 tcgacgtcga aaacggcttc cagaatggac cacactagtc cattccgcta gcggccgcga
     4921 aaaaaactta cgtcgcgagg cggcggcgga agtcgaggcg ggccacgggc ttgtggcaac
     4981 gcatgttcac gtaagtcagc ccacagtgaa caaaatgcgc acaagttgct tgccgacctg
     5041 ggttctgtgg gcacaatgtg gccatgtgtg atcgtgtgga tagtttgtag ggtgggctgt
     5101 gcccaccgtc aaccgaccgc ggtgggcaca gcccactcta cctgttcccg gaggccctca
     5161 tgtcacgtcc gccgaaactc cgaccgctgc ccgagcggga gcgccgcgat gcgctcgccg
     5221 cgtatttgaa agtgctcgat cgcgatggcg agctggatca tttccgggcg ctggtgcccg
     5281 ccgagcagcc ttggagcgat cggccctccg cggcaacgcc gccctcagcc cgcc
//
LOCUS       DASRJB010000839         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1374475, whole genome shotgun sequence.
ACCESSION   DASRJB010000839 DASRJB010000000
VERSION     DASRJB010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374475"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1054
                     /locus_tag="VFB80_22080"
     CDS             92..1054
                     /locus_tag="VFB80_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36541.1"
                     /translation="MATATLELNRPLETQPAAALPVWWRQRHTYVLAAMAAVQVVGCR
                     WSVLLIDGSTWAGEIIVGVLLAQSFLLGLWAALGGLGTLPRWGIIGTIHLAGVVSMSL
                     GMRIFGASLWWAEALQIGLIGAMLVLGFAACLLPLRGLAGWRVDFDAAVYRDVRGRRG
                     QVSFMDLAGYSLGVGAALAAARLTVQAEIIDTEGLLILCTWLSLVLLTGSGVAYALLT
                     WRRLGLALAASVVWPLVIAGGNSLLAEYIEDLDVFGGSANPAVLGVRLHMVSFYGGVA
                     LLVATTLTLLRLFGLKLIVVPLPVEQASSLVHSQATEFTPAKAA"
     gene            complement(1032..2408)
                     /gene="mgtE"
                     /locus_tag="VFB80_22085"
     CDS             complement(1032..2408)
                     /gene="mgtE"
                     /locus_tag="VFB80_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN36542.1"
                     /translation="MVNTLFLPELREMLAENNAFELKEFCTALHPARTAEFMEGLDPP
                     ESWKVLRHAELKLREDIFGYFPKDKQLAIIQAEDRAEVAELLADLPADDRVDLLRAVR
                     PEVVEEILPLLPAHERREILRLRDYPENSAGAMMTTEVAMIDEDLTVRQAIDELSREA
                     ERLETIYYIYAVDKDQHLDGVVTARKLLTALKKPDTKVSDLMECDLIFVNADENRDVV
                     TRRVAKYDLHAIPVVDDRRRLVGIITHDDVIDAFREEATEDAQLAAAVAPLEEGYMQT
                     SVVTLWWKRGVWLTVLFVTAMFTATALHFYEGKLKEYAWLVIFIPLIMGSGGNTGNQS
                     ATLIITALTTGNVLLRDWRRILWREFRVGVMLGLLLALLGVILSWIQTRNDPDAKDHR
                     YWPLVVPLTLVLVVTCGAVSGSLLPLIFRRMGLDPALMSNPFVAALSDILAIVIYMTV
                     ALTLLSLG"
BASE COUNT          445 a          945 c          981 g          515 t
ORIGIN      
        1 tggagagttg acgcggcgat tcgcgtagtg ccgcgcgggc ctatcggcta aactgcccgc
       61 ggttcaaaac gcgtggcagg gaggctgcac catggcaacg gcgacgctcg aactcaatcg
      121 accgctggaa acccagcccg ccgcggcgct gcctgtctgg tggcggcagc ggcacacgta
      181 cgtgctggcg gcgatggccg cggtgcaagt cgtcggctgc cgctggtcgg tgctgctcat
      241 cgatggatcg acgtgggccg gcgagataat cgtcggcgtg ctcctggcgc agagctttct
      301 gctgggcctg tgggctgcgc tcggcgggct ggggacgctg ccccgctggg gaatcatcgg
      361 cacgatccat ctcgcgggcg tggtgtcgat gtcgctcggg atgcgcatct ttggcgcctc
      421 gctgtggtgg gcggaagctc tgcagatcgg attgatcggg gccatgctcg tgctgggctt
      481 cgccgcctgc ctgttgccgc tgcggggact cgccggctgg cgggtcgatt tcgacgcggc
      541 cgtctatcgc gatgtccgcg gccgccgcgg gcaggtgagc ttcatggacc tggccggcta
      601 cagcctcggc gttggagccg cgctggccgc cgcccgcctg acggtgcagg ccgaaatcat
      661 cgacaccgaa ggcttgctga ttctctgcac gtggctcagc ctggtcctgc tgaccggctc
      721 gggggtcgcc tatgccttgc tcacctggcg ccggctcggg ctggcgctgg cggccagcgt
      781 cgtttggccg ctggtcatcg ccggcggcaa ctcgctgctg gcggaatata tcgaggatct
      841 cgacgtcttc ggcggcagcg ccaacccggc ggtcctcggc gtgcggctgc acatggtcag
      901 cttttacggc ggcgtggccc tgctcgtcgc cacgacgctg accctgctgc ggctattcgg
      961 cctcaagctg atcgtcgtgc cgctgcccgt agaacaagcg tcctcgcttg ttcattccca
     1021 ggcgacggag ttcaccccag cgaaagcagc gtgagggcga cggtcatata gatgacgatc
     1081 gccaggatgt cgctgagggc ggcgacgaac gggttgctca tcagtgcggg gtccaggccc
     1141 atccgccgga agatcagggg caacagcgat ccggagaccg cgccgcaagt gacgaccagg
     1201 accagcgtca gcggcacgac cagcggccag tagcggtgat ccttggcgtc cggatcgttg
     1261 cgcgtctgaa tccacgacag gatcacgccc agcagcgcca gcaggaggcc cagcatcacg
     1321 ccaacgcgga actcccgcca caggatcctt cgccagtcgc gcaacaggac gttgcccgtc
     1381 gtgagggccg tgatgatcag cgtcgcagac tggttgcccg tgttgccgcc gctccccatg
     1441 atcagcggga tgaagatcac cagccaggca tactccttga gcttgccttc atagaagtgc
     1501 agggccgtgg cggtgaacat cgcggtcacg aacagcacgg tcagccacac gccccgcttc
     1561 caccacagcg tgaccacgct ggtctgcatg tagccttctt ccagcggggc gacggccgcg
     1621 gcgagctgcg cgtcttcggt ggcctcctcg cggaaggcgt cgatcacgtc gtcgtgcgtg
     1681 atgatgccca ccaggcggcg gcggtcatcg acgacgggaa tcgcgtgcag gtcgtacttc
     1741 gccacgcggc gagtcaccac gtcgcggttc tcgtcggcgt tgacgaagat caggtcgcac
     1801 tccatcaggt ccgagacctt ggtgtccggc ttcttcagcg ccgtcagcag cttgcgggcc
     1861 gtcaccacgc cgtccaggtg ctggtcctta tcgacggcgt agatgtagta gatcgtctcc
     1921 agccgctcgg cctcgcggct gagctcgtcg atggcctggc ggacggtcag gtcctcgtcg
     1981 atcatggcca cttcggtggt catcatcgcg ccggccgaat tctcggggta atcgcggagc
     2041 cggaggattt cccgccgctc gtgggcgggc aagagcggca ggatctcctc gacgacctcc
     2101 gggcgcacgg cgcggagcaa gtccacgcgg tcgtcggccg gcagatcggc caggagctcg
     2161 gcgacctcgg cccggtcctc ggcctggatg atcgccagct gcttgtcctt cgggaaatag
     2221 ccgaagatgt cttcgcggag cttgagctcg gcgtgccgca ggaccttcca ggattcgggc
     2281 gggtcgagcc cctccatgaa ctcggcggtg cgggccgggt gcagcgcggt gcagaattcc
     2341 ttcagctcga aggcgttgtt ctccgccagc atctcgcgca gttcgggcag gaacagcgtg
     2401 ttgaccatgg cggatagtcg cggccagcta gcccgcaatt cggcgcggaa gttctgagta
     2461 tcgggcggtg cgccgcgcct gtcaaaggcg gcccgcaagc atacgtcgcg gcccaaaagc
     2521 aggccactcg ctccgccagg gtcggactca cgcggagcgt aaggacgact atgccgttgt
     2581 gaggccgcgc ctcgcaaagt aagatgaatc gactggtacg gcagtttcct cccgtacgcc
     2641 caccgcgctg atacggcagc ttcctcccgt atgtcccgct cggctctggt ccgagccgcc
     2701 tcaggtccgc tggagtgtgc cggccccatt gtttcggccg gacggcagcg ttgaccggcg
     2761 cagtccgcag gccgcccacg attgcaattc tcggcagccg cgaagagaac gcccctctca
     2821 tggccaagca accccacgac gacctgtttg aaaagagcac gatgtctttc ggcgaccacc
     2881 tggagg
//
LOCUS       DASRJB010000840         7879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2770331, whole genome shotgun sequence.
ACCESSION   DASRJB010000840 DASRJB010000000
VERSION     DASRJB010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2770331"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFB80_22090"
     CDS             <1..639
                     /locus_tag="VFB80_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZN36543.1"
                     /translation="LKGEFVWTKGRPQEPGVYKDPAVKYSPTNVAFGPDGGFYIGDGY
                     GSHYIHQYDKDANWVRTWGGVGTEPGKMRTPHGLWLDNRPGREPTLVVADRANARLQY
                     FTLGGQHLSFVNEVSFPAHFDIRGDVLLVPDLHARVSLFGKDNKVIAHLGYDEAWTKE
                     VLAENMKMRREPARWQPGRFIHPHDACYDADGNIFVVEWVPTGRVSLLRKVS"
     gene            788..1945
                     /locus_tag="VFB80_22095"
     CDS             788..1945
                     /locus_tag="VFB80_22095"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36544.1"
                     /translation="MTELLLQFSPFSLLKEFPMTSVQVSRNHRGFTLVELLVVIAIIG
                     VLVALLLPAVQSAREAARRTQCSNHQKQLVLALHNFHDSLGKFPPLRGGRNDGSNRCG
                     DMFGLVFALPYVEQGPRSDQIANGPAVVSWDNAYAPYMGKMTVLLCPSSPLGQNNPVN
                     SNQPQRSYHLSMGTTVGTNPSVAGFSATITDNNYFGLVDGLFGYEKPGAVIPNCDAPA
                     QVRKMSSITDGTSNTVALSEKVTGTRGSRSVLGMATFPMTGLDTNAAQCLATATNGVY
                     NAGRNLSDWHMGGIWAFGHPNWAGFSTILPPNSPSCYERNGNNPSNSVGVFSVSSHHP
                     GGVMIAMADGSLRFIRQSIDCGNYGVAPNRNYGVWGALGTIAGGEALSDNF"
     gene            2131..2568
                     /locus_tag="VFB80_22100"
     CDS             2131..2568
                     /locus_tag="VFB80_22100"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZN36545.1"
                     /translation="MRRRSLLGLLGLALLVGCNSASVSGTVPVSGKVTYKGAPVEGAI
                     VTFVPEGKGRTATATTGAGGVFSLTTVDAPGAMPGKYKVTVDKVEYGAAGSPSMEEAA
                     KGNAAEGQSKRVLPAKYAEVASTPLAIEVPSGGKKDLDLPLID"
     gene            2669..3949
                     /locus_tag="VFB80_22105"
     CDS             2669..3949
                     /locus_tag="VFB80_22105"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HZN36546.1"
                     /translation="MPEFDTAFQVSMSNLRFGAGATREIGMDLEDLGVRRTLLVIDPG
                     LRSLPAGDAVLSALRASRVAFDVFDQVDVEPTDASFRRAIEVATAGHFDSFVAVGGGS
                     TIDTAKAANLYSTYPAEFLDYVNAPIGRGKPIPGPLKPLIAVPTTAGTGSETTGVAIF
                     DLVEQHAKTGIAHRYLRPLLGIVDPENTRTQPAAVAASAGLDVLSHALESYTAIPYDR
                     RPRPERPLLRPAYQGTNPISDLWAIRALEIVAEFLPRAVSDTSDDEARSQMCLAASMA
                     GIGFGNAGVHLPHGMSYPVAGMVKRFRPSGYAVDHPLVPHGMSVILHTPAVVRFTAPA
                     CPERHLRAATALGADTRGTAPADAGEVLAQRVIHFMRLLKMPNGLAAIGYEASDIPAL
                     VRGTLPQHRVTKLSPVPAGEAELTELFRASLKVW"
     gene            4024..5415
                     /locus_tag="VFB80_22110"
     CDS             4024..5415
                     /locus_tag="VFB80_22110"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZN36547.1"
                     /translation="MNNTRNGLHRRAFLQASLAAGVGGGAAIAAQQPARAGYVTSEVG
                     RLKRVIVHEPGAEVTKAFPLFLGNHSMLTWELLRNDAAKQHQAFVGKMKEAGVEVLDF
                     EKLLEEALTEARRSKRLGTWLAAHAPALVEHEAKLMVDTLLGRDDRFIYRRDATGAMQ
                     PAVSPATVLFFTRDLAVMTPRGLVLGKFEGEVRSFEAALTRFIFQNAPQLAKYQVAFD
                     AAAENVLLQGGDVLVLDDRTLLVGVGNATDEKAAQRLAQKLSLDVVAVQMPSKAWQPG
                     EWEGLQLIFYHLDCLVNLVDLRTALAVPYLLEKAHARQNPILDVLYGFARLSSLPAEQ
                     RLALLDEVRNIGWIRRYQAGSGELDKSLGETKVVDYLKEAGFKVIYAGGERRPDENDL
                     QHVVERVIRGCRFMSVNVLAVRPGELLSYEGNTFTLAALEQAGLKVTTFPSHELVRAN
                     GGPHCLTMPLERD"
     gene            5520..7337
                     /locus_tag="VFB80_22115"
     CDS             5520..7337
                     /locus_tag="VFB80_22115"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN36548.1"
                     /translation="MLAARLKQAGIVVAVAAFVMLTNLGGPRLWDRDEPRNAGCAREM
                     LLRRDWVVPTFNAKLRVDKPVLLYWGIMASYLAFGVNEFAARLPSALAAIGSALCVYI
                     MGRRLFGPRAGVWAGIALATSLSFVLAGRAATPDSLLVLCTCLAMTVFVCGTFRPRFE
                     TTPDDAEPHLVQAGEFFPQHWPTVLAMYAAMGLGVLAKGPVGLVLPTAVIGMFLLLAR
                     LPGRSHEPRATHFMDYLGRIAAPFQPLHFLRTCWCMRPLTAIFAAAAVALPWYWAVGL
                     ATDGAFLDGFFIRHNLERATGVMEGHSGSLLYYPAVMLVGFFPWSVFALPLLIDTVLQ
                     LRRRDRLHHGYLLAVCWVGVFVVLFSLARTKLPSYVTPCYPALALLVGDYVDRWSRQA
                     AAVAGGWLPAAFGCLALAGVGIAIAVPLAAKRYLPGEEWLGLIGLTPLAGGAACLGLA
                     VARNYRAAAGIFAASAVTFVCLVFAVGAQRIDRHQQNHVLLRTIDGHAARPTVASYKI
                     LEPTWVFYGGRPVSELPQKLQSAPPQQVAEFLSRDEAFVITTRSRLPELAPQLPPGIQ
                     VLAEAQLFLRDETLLVLGRPAAGAASNQRHYSLLPKDLR"
     gene            7641..>7879
                     /locus_tag="VFB80_22120"
     CDS             7641..>7879
                     /locus_tag="VFB80_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HZN36549.1"
                     /translation="MEAGEILIRRGLMNRSQLEQTRSMANGHGDGTRLIESAIQLGFV
                     SEEQALKAVGEEVGIEFIDLSEAQVDLSLLKTFPQ"
BASE COUNT         1356 a         2559 c         2508 g         1456 t
ORIGIN      
        1 ctcaaaggcg aattcgtttg gaccaagggc cggccgcagg agccgggcgt ctataaagac
       61 ccagccgtta agtacagccc gaccaacgtg gccttcggac cggacggcgg gttctacatc
      121 ggcgacggct acgggtcgca ctacatccat caatacgaca aggacgccaa ctgggtccgc
      181 acctggggcg gcgtgggcac cgagcccggc aagatgcgga cgccgcacgg cctgtggctc
      241 gacaaccgcc ccggccgcga gcccacgctg gtcgtggccg accgcgcgaa cgcccggctg
      301 caatacttca cgttgggcgg ccagcacctg agcttcgtga atgaggtgag cttccccgct
      361 cacttcgaca tccgcggcga cgtgctgctc gtgcccgacc tgcacgctcg cgtgtcgctg
      421 ttcggcaaag acaacaaggt gattgcccac ctgggctacg acgaagcctg gaccaaggaa
      481 gtcctcgccg agaacatgaa gatgcgccgc gagcccgccc gctggcagcc cggccgcttc
      541 atccaccccc acgacgcctg ctacgacgcc gacggcaaca tcttcgtcgt cgagtgggtg
      601 cccacggggc gggtctcgtt gctacgcaag gtttcgtagc gatttcctcg ccagtcccgt
      661 acatcgcttc gaccccccgc gaagtcctca cccaaaacgc tgcccgccgc ggtgtcgcag
      721 ttggagattg acactcccaa aagttgcggc aatgtcgccg ccagatataa actgccgatt
      781 agtgaagatg acggaattgc ttcttcaatt ctctccattt tcacttttga aggagtttcc
      841 aatgacgtca gtccaagtca gtcgcaacca tcgaggcttc acgctcgtcg agttgctggt
      901 ggtcattgcc atcatcggag tcctggtcgc tttgctgctg ccggcagtgc aatccgcccg
      961 cgaagccgcg cggagaacgc agtgctcaaa ccatcagaaa cagctcgttc tcgcgctgca
     1021 taacttccac gattcccttg ggaagtttcc tccgctgcga ggaggtcgca acgacggctc
     1081 caatcgctgc ggcgatatgt tcggcctcgt gttcgcgctg ccctacgtcg agcaagggcc
     1141 acgatccgac cagatcgcga acgggcctgc cgtggtttct tgggacaacg cctatgctcc
     1201 atatatggga aaaatgacgg tcctgctttg tccctcgtcc cctctcggac agaacaatcc
     1261 ggtgaactcg aatcaaccgc agcgcagcta ccacttgtcc atgggcacga ccgtcggaac
     1321 caacccctct gtcgcaggat ttagcgcgac catcaccgac aacaactatt tcggtctcgt
     1381 ggacggactc ttcggctatg aaaaaccggg cgccgtgatc cccaattgcg acgcacccgc
     1441 acaggtaagg aagatgtctt cgattaccga tggcacgagc aatacggtcg cgttgtccga
     1501 aaaagtcaca ggaacccgag gttctcgttc ggttctcggc atggcgacgt ttccgatgac
     1561 gggtctggat acgaatgccg ctcagtgtct ggccaccgcg accaacggcg tttacaacgc
     1621 gggccggaac ctgtccgatt ggcatatggg cggcatttgg gccttcggtc accccaattg
     1681 ggccggtttc tccacgatct tgcccccgaa cagcccttct tgctacgaac gcaacggtaa
     1741 caatccgagc aattcggtgg gcgtcttctc ggtcagcagt caccacccag gcggcgtcat
     1801 gatcgcgatg gcagacggaa gcttgcgctt catccggcag tcgatcgact gcggcaatta
     1861 cggcgtcgct cccaatcgca actacggggt gtggggtgcg ctcggcacga tcgcgggtgg
     1921 tgaagcccta tcggacaact tctaatagcg cctaacaata acccgaagcg tcagccaggg
     1981 acttcatgag tcaacgcgat ttcacaaagt ccctcgctga cgcttcgggt taatgtgagt
     2041 tgtgctacgc actctcattc gctatccgcg agaatcgcct ggacgaccgc gggtcgccca
     2101 ctgttttccc ctttttcctt ggaacagatt atgcgtcgcc gctccctgct tggactcctt
     2161 ggccttgctt tgctggtggg gtgcaattcc gcgagcgttt ctggaacggt tccagttagc
     2221 ggcaaagtga cctacaaggg ggcgcccgtc gaaggcgcga tcgtcacctt cgttccagaa
     2281 ggcaaaggtc gcaccgcaac tgcgacgacg ggagcgggcg gtgtgtttag cctgacgacg
     2341 gtcgacgccc cgggcgcgat gcctggaaaa tacaaagtca ccgtcgataa ggtggaatac
     2401 ggagccgcag gttctcccag catggaagag gccgcgaagg gaaatgccgc cgaggggcag
     2461 tcgaagcgcg tgttgcccgc gaaatatgcc gaggttgcgt cgactccgct cgcgatcgag
     2521 gtcccctcgg gcggcaagaa ggatctcgac ctccctctca tcgattgaat cgtacacctg
     2581 ctgcgtacca acggggcgag tgacgctcct tcgggaggtc tcatagcgcc gcggcggcta
     2641 ccaactgccg cagcacggga ataccggcat gcccgaattt gataccgcct tccaggtctc
     2701 catgtcgaac ctgcgcttcg gcgcgggggc aacgcgggag atcggcatgg acctggagga
     2761 cttgggcgtc cggcggacgc tgctggtgat cgatccgggt ttgcgcagcc tgccggcggg
     2821 cgacgcggtg ctctcggcgc tgcgggccag ccgcgtggcg ttcgacgtct tcgaccaggt
     2881 cgatgtcgag ccgaccgacg ccagctttcg gcgggcgatt gaagtcgcga cggcgggcca
     2941 tttcgattcg ttcgttgccg tcggcggcgg cagcacgatc gacacggcca aggcggcgaa
     3001 cctttactcg acgtatccgg ccgagttcct cgactacgtc aacgcgccga tcggccgcgg
     3061 caaaccgatc cctggacccc tgaagcccct gatcgcggtg ccgacgactg ccggcaccgg
     3121 cagcgagacg accggcgtgg cgatcttcga cctggtcgag cagcatgcca aaacgggcat
     3181 cgcccaccgc tacttgcggc ccctgctggg catcgtcgat cccgaaaaca cgcgcacgca
     3241 gccggcggcg gtggccgcgt cggcggggct cgacgtgctg agccacgcgc tggagtccta
     3301 cacggccatt ccgtacgacc gccgcccgcg gcccgagcgg ccgctcttgc ggccggccta
     3361 tcaaggcacc aatccgatca gcgatctgtg ggcgatccgc gccttggaga tcgtcgccga
     3421 gtttctgccc cgagcggtca gcgataccag cgacgacgag gcccgcagcc agatgtgcct
     3481 ggcagcttcg atggctggca tcggcttcgg caacgctggc gtccacctgc cgcacggcat
     3541 gtcgtatccc gtggcgggaa tggtgaagcg cttccgcccg agcggctatg cggtggacca
     3601 cccgctcgtg ccgcacggca tgtcggtcat tctgcacacg cccgccgtcg tgcgattcac
     3661 ggccccggcc tgccccgagc gccacttgcg ggccgccacc gccctcgggg ccgacacgcg
     3721 cggcacagcg ccggccgacg caggcgaagt cctggcccag cgggtcatcc atttcatgcg
     3781 gctgctaaag atgccaaatg gcctggccgc gatcggttac gaggccagcg atattccagc
     3841 gctcgtccgc ggcactttgc cgcagcaccg cgtgaccaag ctctcgccgg tccccgcagg
     3901 cgaggcagaa ctgacagaac tgttccgcgc gtcgctgaaa gtctggtagt gcggcggcaa
     3961 tccccgcgcc gccatacgtg aatcgggctg caagtggtaa gttcctggct gggggacgag
     4021 gctatgaaca atactagaaa cggcctgcat cgccgcgcgt tcctgcaagc ttcgctggcc
     4081 gcgggcgttg gcggcggcgc ggcgatcgcg gcacagcagc cggccagggc gggctacgtc
     4141 acctcggaag tcggccggct caagcgcgtg atcgtgcacg agccgggcgc ggaagtgacc
     4201 aaggcatttc cgctgttcct tggcaatcac tcgatgctca cctgggagtt gctgcggaat
     4261 gacgcggcga aacagcatca ggcgttcgtc ggcaagatga aggaggccgg cgtcgaagta
     4321 ctcgacttcg agaagctgct cgaagaggcg ctgacggaag cccgccggtc gaagcgattg
     4381 ggcacgtggc tcgccgcaca tgccccggcg ctcgtcgagc acgaagcgaa gctgatggtc
     4441 gatacgctgc tggggcgcga cgaccgcttc atctatcgcc gcgacgccac tggggcgatg
     4501 cagccggcgg tcagtccggc cacggtgttg ttcttcacgc gcgacctggc cgtgatgacg
     4561 ccgcggggcc tggtgctggg caagttcgag ggagaagtgc gttcgttcga ggcggccctc
     4621 acgcggttta tcttccagaa cgcgccgcag ctggccaaat accaggtggc gttcgacgcg
     4681 gccgccgaga acgttctgct gcaaggcggc gacgtgctcg tgctcgacga tcgcacgctg
     4741 cttgtcggtg tgggcaacgc cacggacgaa aaggcggccc agcgactggc ccagaagctc
     4801 tctctggacg tggtcgccgt gcagatgccg tccaaggcgt ggcagccggg cgagtgggaa
     4861 gggctccagc tgatcttcta tcacctcgac tgcctggtga acctggtcga tctccgcacg
     4921 gcgctggcgg tgccctacct gctggaaaag gcccatgccc ggcagaaccc gatcctcgac
     4981 gtgttgtacg gctttgcccg gctgagcagc ctgcccgccg agcagcggct ggccctgctc
     5041 gacgaggttc gcaacatcgg ctggattcgc cgctatcagg ccggctcggg cgagctcgac
     5101 aagtcgctgg gcgaaaccaa ggtcgtcgat tacctcaagg aggccggctt caaggtgatc
     5161 tatgccggcg gcgagcggcg gccggacgaa aacgacctcc agcacgtcgt cgagcgggtg
     5221 attcgcggct gccgcttcat gagcgtgaac gtgctcgccg tgcggccggg cgagctgctg
     5281 tcgtacgaag gcaatacctt cacgctggcc gccctggagc aggctggcct gaaggtgacg
     5341 acgttcccct cgcacgaact cgtccgcgcc aacggcggcc cgcactgcct gacgatgcca
     5401 ctggagcggg attagccggg cgattgctat gggcttgggc agccaaattc gccagaatcg
     5461 ccctacgaac ccacgcgtct gctagcgtgg cggcgcccta aggatcaagg aggatcgccg
     5521 tgcttgctgc gcgtttgaaa caggcgggaa tcgtagtcgc ggtggcggca ttcgtgatgc
     5581 tcacgaatct gggcggcccg cgattgtggg accgcgatga gccgcggaat gccggctgcg
     5641 cgcgagagat gctcctgcgg cgggactggg tcgtgcccac gttcaacgcc aagctgcggg
     5701 tggacaagcc ggtgctcctc tactggggca tcatggcttc ttacctcgca ttcggcgtga
     5761 acgaatttgc agcccggctg cccagcgctc tggcggcgat cggctcggcg ctgtgcgtgt
     5821 atatcatggg tcggcggcta tttggtccgc gggccggcgt ctgggcgggg atcgcgctgg
     5881 ccacgagcct gtcgttcgtg ctggccgggc gcgcagcgac gcccgattcg ctgctggtat
     5941 tgtgcacctg cctggcgatg acggttttcg tgtgtggcac gttccggccg cgctttgaaa
     6001 cgacgcccga cgatgccgag ccgcacctgg ttcaagcggg cgagttcttc ccgcagcact
     6061 ggccgaccgt gctggcgatg tatgccgcga tgggcctggg cgtgctggcg aagggacctg
     6121 tggggctggt tctgccgaca gccgtgatcg gcatgttcct gctcctcgcc cggctgccgg
     6181 ggcgctcgca cgagccgcga gccacccact tcatggacta cctcggccgc atcgccgcgc
     6241 cctttcagcc gctgcacttc ctgcggactt gctggtgcat gcgtccgctg acggcaatct
     6301 tcgcggccgc ggccgtggcc ctgccgtggt actgggccgt gggtctggcc acggatggcg
     6361 cgttcctcga tggcttcttc atcaggcaca acctggagcg ggcgaccggc gtgatggaag
     6421 ggcacagcgg ctcgctgctc tactatcccg cggtcatgct ggtcggcttc tttccctggt
     6481 cggtgtttgc attaccgcta ttgatcgaca cggtgctgca gctccgcagg cgcgaccgct
     6541 tgcatcacgg ttacttgctc gccgtttgct gggtaggtgt gttcgtcgtg ctcttctcgc
     6601 tcgcccgcac caagctgccc agctatgtca caccctgcta tcccgcgctc gcgctgctcg
     6661 tcggcgacta cgtcgatcgc tggagccgcc aggccgcagc cgtcgccggc ggctggctgc
     6721 ctgcggcttt cggctgcctc gcgctggcgg gagtcggcat cgcgatcgcc gttccactgg
     6781 ctgccaagcg atacctgccc ggtgaagaat ggctgggcct gatcggcctc acgccgctgg
     6841 ctggcggagc ggcctgcctg ggccttgcgg tcgcccgcaa ctaccgcgcg gcagctggaa
     6901 tcttcgcagc cagcgccgtg acatttgttt gcctcgtctt cgccgtgggt gctcagcgga
     6961 tcgaccgcca tcagcaaaac catgtgctcc tgcggacgat tgacggccat gcagcgcggc
     7021 cgacggttgc ctcttacaaa atccttgagc caacctgggt cttctatggc ggcaggcccg
     7081 tctcggagct gccgcaaaaa ctgcaaagtg ccccgccgca acaagttgcg gagtttctaa
     7141 gtcgcgatga agcattcgtc attaccaccc gcagccgctt gcccgaactt gccccgcagc
     7201 tgccgcccgg aatacaagtc ctggcggaag cccaactgtt cctccgcgac gaaacccttc
     7261 tagtgctggg acgcccagca gcgggcgccg caagcaacca gcggcattac tctttgctcc
     7321 caaaggactt gcgctaataa tcgggcggtc cttgattctc atctagccaa ccggaacttt
     7381 tggccgctca agcggttcaa ttgtctcagc agcaagtctg gccgttgggc gattgcgcga
     7441 ggttcggggg agcggctggc gagcgagtag ctgacttggt gccgcccctc atgcagcacg
     7501 gctgaacgca accgctgaag cgggttggat ttgcagcggg atcggaaacc cctcatcgcg
     7561 acttggggta gtgtccgggc gatacgtcca gtaacttgaa cttccctcct gcgctccccc
     7621 tcctgcctga acccacccga atggaagccg gcgaaattct catccgacgc ggcctgatga
     7681 acaggagcca gctcgagcag actcgcagca tggcgaatgg gcacggcgac ggcacgcggc
     7741 taatcgagtc ggcgatccaa ctggggttcg tttctgaaga gcaggccctg aaagccgtcg
     7801 gagaggaagt cggcatcgag ttcatcgacc tgtccgaggc ccaggtcgat ctgtcgctgc
     7861 tcaagacgtt tccccagaa
//
LOCUS       DASRJB010000841         7691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1647067, whole genome shotgun sequence.
ACCESSION   DASRJB010000841 DASRJB010000000
VERSION     DASRJB010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647067"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFB80_22125"
     CDS             <1..273
                     /locus_tag="VFB80_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HZN36550.1"
                     /translation="LEELFDNGSQVTPDVLQAAGIVKRRFDVLKVLGDGELTKKLSVS
                     AHRFSASAKEKIEKAGGTVTILPGRTPAEEKKKAKKLAKKQAAAAK"
     gene            420..1817
                     /gene="secY"
                     /locus_tag="VFB80_22130"
     CDS             420..1817
                     /gene="secY"
                     /locus_tag="VFB80_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN36551.1"
                     /translation="MWEKFRAIFTIPELRQKILFTLILLAVFRVGYQIPLPVIDQTAL
                     ASNMKRSEGTEWFKFMQGVSIYSAADLRRITIFGLGIMPYISASIILQLLGSVYKPLE
                     DLRKEGETGRKKINEYTRYLTVLMCFIQGAVYLYSSLGTHQTSNIAREFQTASGGLHP
                     LWYMTMICVMTCGTMFVMWLGEQIDEFGIGNGISLLIMAGILAQMPNALIDLLTQVSK
                     NGWRLGGMNEIGVEHLVILSVLFVVVVAGVVFMQLGQRQIPTQSAKHVRGRRVYGGNR
                     QFLPLRLNQSGVMPIIFASSLLMLPSILFNALASPLTGGEPGTATYFFYNLFSTIGSM
                     FGRGTSFVYNVSYIAMIYFFSYFWMAITFNPKEIAENLKDMGTFIPGYRPGRRTAEYL
                     EKVMIRITYVGAAFLALVAIVPTIVSGWLKVDQNIASFYGGTGLLIAVSVAFDLIQKI
                     DSHLVMRDYRGLLEP"
     gene            1861..2436
                     /locus_tag="VFB80_22135"
     CDS             1861..2436
                     /locus_tag="VFB80_22135"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZN36552.1"
                     /translation="MLIVIIGPPGSGKGTQARRLLAHLGVPHISTGDLLRAAKQSGTE
                     LGRQAAKFMDEGRLVPDDLVLAMVAEKLQEPQFDKGGLFDGFPRTIYQAKTLDVSLRE
                     RGTPLAMAVELKADEEELTARILKRAAIEKRTDDTPETIKQRMEVYRKQTAPLLDYYR
                     GQGKLVSIDALGTPDEVFGRIRAAIDKRRPA"
     gene            complement(2469..2825)
                     /locus_tag="VFB80_22140"
     CDS             complement(2469..2825)
                     /locus_tag="VFB80_22140"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN36553.1"
                     /translation="MSQITRLGVSRRWSDVVIHNGVAYWVEVASDTSQDAAGQVAQVL
                     KQIDGTLAEIGSSRLDLLEVLIYLADLDDAAALNEHWDVWIPAGHAPVRACVGATLSP
                     GCLVEMVITAAATNET"
     gene            complement(2822..4612)
                     /locus_tag="VFB80_22145"
     CDS             complement(2822..4612)
                     /locus_tag="VFB80_22145"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HZN36554.1"
                     /translation="MDIVWIAGIIVTAVTSVPVLWQLNRHPKGLFILFFAEMWERFSY
                     YGMRGLLIFFMTQHFLRDDTFSQGQYGAYTSLVYLMPLIGGFLADKFLGTRKAIAFGA
                     ILLVAGHFLMGFEGPPARQILKYQGREYAFQVTGRMEAREVKLIVDDKPYDFGSNIFG
                     EFVIKELPEGASLPPTLKKGAYELLIAGREPLYTNIFYLALSLIILGVGYLKPNISTI
                     VGQLYEQNDPRRDPGFTLYYYGINLGAFWAAIACGWLGTNVGWWAGFGAAGVGMALGF
                     IVFVLGKSWLEGKGEPPNLDLLQRPLAGPLNLEWLIYIVSILCVGLIWLVVQRHAVVG
                     YLLGAGSAIVLGYLLFFMVTKASWIESQRMILALVLIGASVVFWTLFEQAGSSLNQFA
                     ERNTVLEVGSRKISAAQTQSFNAGFILLLAPVFSAIWAYLGKFNMDPNPALKFGLALV
                     QVGAGFFILVWGANFADQSYRVPLSFLVFAYLLHTTGELCLSPVGLSQMTKLSPAAVV
                     STIMATWFVASSWAQYVGGMVAKLTAAETVAGQVLDPAKALQTYVGVFKMIGIWGLAI
                     GAGLIILSPVLRYLAHDAPADAAPPDKAAT"
     gene            complement(4687..5928)
                     /locus_tag="VFB80_22150"
     CDS             complement(4687..5928)
                     /locus_tag="VFB80_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HZN36555.1"
                     /translation="MRAEIIAIGDEMTTGQRLDTNSQWLAERLLEIGVPVAFHTTVGD
                     QLADNVLVFRQACERADIVVASGGLGPTADDLTRQALADLAGVPLVQDDAMLEHIKAL
                     FARRKRAMPPANVVQALFPQGSRPIPNPHGSAPGIDIEIPRAGGKSARVFAVPGVPAE
                     MKEMWAASVGPAIRERLGIRKVIAHHRLKCFGVGESDLEAMLPDLIARQRYPLVGITV
                     SQATITLRITAEGETAEAARAAMQPTIETIEQCLGDLIFGEEDEELEHAVIRRLLDRR
                     ETLAVVEWGTGGVLANWLSECPGSSGVFEGGLVVRNHVALESMLPVRPEAWRNEGFRS
                     ASFTGELARAALERFGTDWVLVVGPFPEDERDIAVFLGMATKDGVITKPITFAGHPSI
                     LKDRTAKQALDWLRKRATPPT"
     gene            6045..>7691
                     /locus_tag="VFB80_22155"
     CDS             6045..>7691
                     /locus_tag="VFB80_22155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZN36556.1"
                     /translation="MKEDVLEIGGMHCASCVARVEGALTKVPGVESARVNLLTRQGRV
                     TYDPARADLQRLAQAVGAAGYEARPITAASGTTSQLADQTRNEVSYWQSRLVWAIALL
                     VPLIAAHFLAARYHFAASMAQLVLATAMQLIVGWPYLAGALKQLRHGGANMDSLIALG
                     TTAAYGAGIADLLRGAHGMNFMDGAMILAFITLGKYLEARAKGRASQAILKLIELAPP
                     VAHVERNLALVDLPLEKVEPGETIVVRPGDRVPLDAVVTGGTSDLDEAWLTGESLPVT
                     KRAGDTIYAGTINGSGSLRARVSRSSGETWLAQTVELVRRAQESKADVQRLADSVVAW
                     FVPAVLAIAAITLIAWIVVSPAHWQLGISAAVAVLVVACPCALGLATPAAVLVASGRG
                     AENGILIKDAPSLETAARLTTVVLDKTGTITLGQPKVIAVQPALGQSAERAIAIAAAA
                     ERHSSHPLAHAIVRHAEQQQVPVIEASEVEVIPGLGIAARSREGRILVGRSGELAELE
                     AGQAALEVSLEGQPLGRIVLADSVSETSRAAISELKQLGLS"
BASE COUNT         1435 a         2424 c         2521 g         1311 t
ORIGIN      
        1 ctggaagagc tgttcgacaa tggcagccag gtgactccag acgtgctcca ggcggccggc
       61 atcgtcaagc ggcggttcga cgtgctcaag gtgctcggcg acggcgaact cacgaagaag
      121 ctctccgtct cggctcaccg cttcagcgct tcggccaagg agaagattga aaaggccggc
      181 ggcacggtca cgattctgcc cggccgtacg cctgccgagg aaaagaagaa ggccaagaaa
      241 ctggccaaga agcaggcggc agccgcgaag tagccctaag cggcccaaat taagggcccc
      301 aaaacccgca tggtcccgcc ggggcgagtt tggtaattta gccagtcgtg gcagactata
      361 gcgcccgcag tcgcctccgc gctgcgttgg cgggaacgat ttcgtaaggc acccaggcca
      421 tgtgggaaaa attccgcgcg atcttcacca ttcccgaact ccggcagaag atcctcttca
      481 cgctgatcct gctggcggtg ttccgcgtgg gctatcagat tccgctgccg gtcatcgatc
      541 aaaccgcgct ggccagcaac atgaagcgca gcgaaggcac ggaatggttc aagttcatgc
      601 agggcgtgtc gatctacagc gccgccgacc tgcggcggat cacgatcttc ggcctgggca
      661 tcatgcccta catctcggcg tcgatcatcc tgcagcttct gggcagcgtc tataagccgc
      721 tggaagacct ccgcaaggaa ggcgagaccg gccgcaagaa aatcaacgaa tacacgcgct
      781 acctgaccgt gctgatgtgc ttcattcagg gggccgtcta cctctattcg tccctcggca
      841 cgcaccagac ctcgaacatc gcccgcgagt tccagacggc cagcggcggc ctgcatccgc
      901 tctggtacat gaccatgatc tgcgtcatga cctgcggcac gatgtttgtg atgtggctcg
      961 gcgagcaaat cgacgagttc ggcatcggca acggcatcag cctcctgatc atggctggca
     1021 tcctggccca gatgcccaac gccctgatcg acctgctcac gcaggtcagc aagaacggct
     1081 ggcggctggg cggcatgaac gagatcggcg tcgaacacct ggtgatcctg tcggtgctgt
     1141 tcgttgtcgt cgtggccggc gtggtcttca tgcagctcgg ccagcggcag attccgaccc
     1201 aaagcgccaa gcacgtccgc ggccggcggg tttacggcgg caaccgccag ttcctgccgc
     1261 tgcggctgaa ccagtcgggc gtgatgccga tcatcttcgc cagtagcctg ctgatgctcc
     1321 cctcgatcct gttcaacgcc ctggcctcgc cgctgacggg cggcgagccg ggcaccgcga
     1381 cctatttctt ctacaacctg ttctcgacca tcggcagcat gttcggccgc ggcacgtcgt
     1441 tcgtctacaa cgtctcgtac atcgcgatga tctatttctt cagctacttc tggatggcga
     1501 tcaccttcaa tccgaaggaa atcgccgaga acctgaagga catgggcacg ttcattcccg
     1561 gctatcgccc ggggcggcgg accgccgagt acctggagaa ggtgatgatc cgcatcacct
     1621 atgtcggcgc ggcgtttctg gccctggtgg cgatcgttcc gaccatcgtc tccggctggc
     1681 tcaaggtgga ccagaacatt gcgagtttct acggcggaac cggtctgctc atcgcggtga
     1741 gcgtggcctt cgacctgatc cagaaaatcg acagccacct ggtgatgcgc gattaccgcg
     1801 gcctgcttga gccgtaggct tttgtcagtc gtcagtggtc agtagtcagt ggctttgacc
     1861 atgctgatcg taatcatcgg tcctccgggg tcgggcaaag gcacgcaggc caggcggctg
     1921 ctggcccacc tgggcgtgcc gcacatctcc acgggggact tgctgcgggc cgcgaagcag
     1981 agcggcactg agctcggccg ccaagccgcg aagttcatgg acgaagggcg gttggtccca
     2041 gacgacctgg tcctggcgat ggtcgccgag aagcttcagg agccgcagtt cgacaagggc
     2101 ggcctgttcg acggctttcc gcgaacgatt taccaggcca aaacgctcga cgtgtcgctc
     2161 cgcgagcggg ggacgccgtt ggcgatggcc gtggagctga aggccgacga ggaggaattg
     2221 accgcacgga tcctcaagcg ggccgcgatc gagaagcgga ccgacgacac gcccgagacg
     2281 atcaagcagc ggatggaggt ctaccggaaa cagactgcgc cgctgctgga ctactaccgc
     2341 ggccagggca agctcgtctc gatcgacgcc ctcggcactc ccgacgaagt ctttggccgc
     2401 atccgcgccg ccatcgacaa gcggcggcca gcgtaggaga accgctaatg ccgcggcatc
     2461 gcctacgcct aagtctcatt cgtggccgcg gcggtaatca ccatctccac caggcagccg
     2521 ggcgacaggg tggcgcccac gcaggcgcgg accggggcat ggccggcggg aatccaaacg
     2581 tcccaatgct catttaacgc cgcggcgtcg tccaggtcgg cgaggtagat gagaacctcc
     2641 agcagatcca ggcggctgct gccaatttcc gcgagggtcc cgtcgatctg tttcagcact
     2701 tgggcaacct gtccggcggc atcctggctt gtgtcggaag caacctcgac ccagtaagcg
     2761 acgccgttat gaataacgac gtccgaccag cggcgggata cgcccaggcg agtgatctgg
     2821 ctcatgtggc ggctttgtcg ggcggcgcgg cgtctgccgg cgcatcgtgg gcgaggtagc
     2881 gcaggacggg cgagaggatg atgagtcctg caccgatcgc caggccccag atgccgatca
     2941 tcttgaacac gccgacgtag gtctgcaggg ccttggcagg gtccagcacc tggccggcga
     3001 cggtttcggc ggcggtaagc ttggcaacca tcccgccgac gtactgggcc caggaagatg
     3061 ccacgaacca ggtggccatg atggtcgata cgactgccgc gggcgagagc ttggtcatct
     3121 gcgacaagcc gactggcgag aggcacaatt caccggtggt gtgcagcagg taggcgaata
     3181 ccaggaagct cagcggcacc cgatagctct ggtcggcaaa gtttgcgccc cacacgagaa
     3241 tgaagaagcc cgcgccgacc tgcaccagcg ccagcccgaa cttaagcgcc ggattggggt
     3301 ccatgttgaa cttgccgaga taggcccaaa tcgccgagaa gaccggcgcc aagagcaaga
     3361 tgaagccggc gttgaacgat tgcgtctggg cggcggagat tttcctgctg cccacctcca
     3421 agaccgtgtt ccgctcggcg aactggttga gcgacgagcc ggcctgctcg aacagcgtcc
     3481 agaaaacgac cgacgcgcca atcagcacca aggccaggat catccgctgc gactcgatcc
     3541 agctggcctt ggtcaccatg aagaacagca agtagccgag gacaatggcc gagcccgcgc
     3601 cgagcaggta gccgacgacg gcgtgccgct gcacaaccag ccagatcagg ccgacgcaga
     3661 gtatgctgac gatgtagatg agccattcca gattcagcgg cccggcgagc ggccgctgga
     3721 gcaaatccag attgggcggc tcgcccttgc cttccagcca ggacttgccc agcacgaaga
     3781 cgatgaagcc gagggccatt ccgacgccgg cagcgccgaa gccggcccac caaccgacgt
     3841 tcgttcccag ccagccgcag gcgattgccg cccagaacgc gccgaggttg atgccgtagt
     3901 aatagagcgt gaagcccggg tcgcggcgcg gatcgttctg ctcgtaaagc tggccgacga
     3961 tcgtcgagat attgggcttg agatagccga cgcccaaaat gatgagcgac agggccaggt
     4021 agaagatgtt ggtgtaaagc ggctcgcggc cggcgatcaa cagctcgtag gcccccttct
     4081 tcaaagtcgg cggcagcgac gcgccctcgg gcagttcttt gattacaaac tcgccgaaga
     4141 tattggagcc aaagtcgtaa ggtttgtcat cgacgatcag cttgacctcg cgggcttcca
     4201 tgcggccggt tacctggaag gcatactccc gcccctggta tttgaggatt tgccgagcgg
     4261 gcggcccttc gaagcccatc aggaaatgcc cagcgacgag caggatcgcg ccgaaggcga
     4321 tggccttgcg cgtgcctaga aacttatcgg ccaagaagcc gccgatcagc ggcatcagat
     4381 agacgagcga cgtgtacgcg ccgtactgcc cctgcgaaaa ggtgtcgtcc cgcaggaagt
     4441 gctgggtcat gaagaagatc aacaggccgc gcatgccgta gtaggagaag cgctcccaca
     4501 tttcggcgaa gaacaggatg aacaggccct tggggtggcg gttgagctgc cacaacaccg
     4561 gaacggaggt gacggcggtg acgatgatcc cggcgatcca gacgatgtcc atgagcggct
     4621 tcctgaggca gtaaacccgg ggctggcccg cattgtagtg cagcagccgc gtaacgccac
     4681 aacgaatcag gttggcggcg tcgcgcgttt gcgcagccag tccagggcct gcttggcggt
     4741 gcggtctttc aggatcgatg gatgcccggc gaaggtgatc ggcttcgtaa tgacgccgtc
     4801 tttggtcgcc atgccgagaa ataccgcaat gtctcgctcg tcctcgggaa acggcccgac
     4861 gaccagcacc cagtcggtgc caaaccgctc gagggcggcg cgggccagct cgccggtgaa
     4921 gctggcgctg cgaaacccct cgttgcgcca agcttcgggg cggaccggca gcatactttc
     4981 cagcgccacg tgattgcgca cgaccaggcc gccttcaaac acaccgctgg agcccgggca
     5041 ttcgctcagc cagttcgcca gcaccccgcc cgtgccccat tcgacgacag ccagcgtctc
     5101 gcggcggtcc agcagccggc ggatgaccgc gtgctccagt tcctcgtctt cctcgccgaa
     5161 gatcagatcc cccaaacatt gctcgatcgt ctcgatcgtc ggctgcatag cggcgcgggc
     5221 ggcctccgcc gtttcgccct cggcggtgat gcggagcgtg atggtcgcct ggctgacggt
     5281 aatgccgacg agcggatagc gctgcctggc aatcaaatcg ggcagcatcg cctccaggtc
     5341 gctctcgccg acgccgaaac acttgaggcg gtgatgggcg atgaccttgc ggatgcccag
     5401 gcgctcgcga atcgccgggc cgaccgacgc agcccacatc tccttcatct cggctggcac
     5461 gcccggcacg gcaaagacgc gagccgattt gccgccggcg cgcgggattt cgatgtcaat
     5521 gcccggcgcg gagccgtgtg gattggggat cggccggctg ccttgcggaa agagcgcttg
     5581 gacgacgttc gccggcggca tcgcccgttt gcggcgggcg aagagggcct taatgtgctc
     5641 cagcatcgcg tcgtcctgta ccagcggcac gccggccagg tcggcgaggg cctgccgagt
     5701 caggtcgtcg gccgtcggac ccaggccgcc gctggctacc acgatgtcgg cccgctcgca
     5761 ggcctggcga aacacgagca cgttgtcggc gagctgatcg ccgaccgtcg tatgaaacgc
     5821 gaccggaaca ccgatttcca gcagccgttc ggccagccac tggctgttgg tgtccagccg
     5881 ctgtccggtc gtcatttcgt cgccgatggc gataatctcg gcccgcatgg gaaaaatcca
     5941 agtaccgaag agccaagaac caaggaggtt gcctgtttgg ttcttcggtt ctttggttct
     6001 ttggtcgttc ggtttccccc gaagtctata attcctgcac cgcgatgaaa gaggacgtgt
     6061 tggagatcgg cgggatgcac tgcgccagtt gcgtggcgcg ggtggaaggt gcgctcacga
     6121 aggtgccggg tgtggagtcg gcccgggtca atctgctgac caggcagggg agagtcacgt
     6181 acgatccggc gcgggccgac ttgcagcggc tggcccaggc ggtcggtgca gccggttacg
     6241 aggccaggcc gataacggcg gcgagcggga cgaccagcca gcttgccgac cagactcgca
     6301 acgaagtgtc ctactggcaa agtcggctgg tatgggcgat tgctctcttg gtcccgctga
     6361 ttgcggctca ttttctggcg gcgcggtacc actttgccgc gagcatggcg cagctcgtcc
     6421 tggcgacggc gatgcagttg atcgtcggct ggccctatct ggccggcgca ctcaagcagc
     6481 tgcgccacgg cggggccaac atggactcgc tcatcgcgct gggcaccacg gcagcctatg
     6541 gcgcgggaat cgccgacctg ctgcgcggcg cgcacggcat gaacttcatg gacggcgcga
     6601 tgatcctggc gttcatcacg ctgggcaagt atctggaggc ccgcgcgaag ggccgcgcgt
     6661 cgcaggcgat cttgaagctg attgagctcg cgccgccggt ggcccatgtc gagcgaaacc
     6721 tggcgctggt tgacctgcca ctggagaaag tcgagccggg agagacgatc gttgtgcgac
     6781 ctggcgaccg cgtgccgctc gatgccgtcg taaccggcgg gacgagcgat ctcgacgaag
     6841 cctggcttac gggtgagtcg ctgccggtaa ccaaacgcgc cggcgacacg atctatgccg
     6901 gcacgatcaa cggcagcggc tcgctgcggg cccgcgtcag ccgttcgtcc ggcgagacgt
     6961 ggctcgcgca gacggtggag ctggtccgcc gcgcccagga gtcgaaggcc gacgtgcagc
     7021 ggctggcgga tagcgtcgtc gcttggtttg taccggcggt gctggcaatc gccgcgatca
     7081 cgttgatcgc ctggattgtc gtatcgccgg cccattggca actgggcatc tctgctgcgg
     7141 tggcggtgct cgtggtggcc tgcccctgcg cgctgggcct ggccacgccg gccgcggtgc
     7201 tcgtcgcctc cggacgcggc gcggagaacg gcatcctcat caaagacgcg ccgtcgctcg
     7261 aaacggccgc ccggctgacg acggtcgttc tcgacaagac cggcacgatc acgctcggtc
     7321 agccgaaagt catcgctgtg caacccgcgc taggccaatc ggccgagcgc gccatcgcca
     7381 ttgccgcggc cgcggaacgg cacagcagcc acccgctggc gcacgctatt gtgcggcatg
     7441 ccgagcagca gcaagtccca gtaattgagg ctagcgaggt cgaggtgatt ccgggactgg
     7501 gcattgccgc gcggagccgc gagggccgga ttctggttgg ccggtcgggc gaattggccg
     7561 agttggaagc gggtcaagcc gcgctcgaag tttccctgga gggtcaaccg ctcggccgca
     7621 ttgtgcttgc cgacagcgtg tccgaaacga gccgggccgc cattagcgaa ttgaagcagc
     7681 tcggcctgag c
//
LOCUS       DASRJB010000842         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3352297, whole genome shotgun sequence.
ACCESSION   DASRJB010000842 DASRJB010000000
VERSION     DASRJB010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352297"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFB80_22160"
     CDS             complement(<1..287)
                     /locus_tag="VFB80_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36557.1"
                     /translation="MLSTIPSDDGRLLAATADWLAGVGDLNVEALAQLAADQGIDFAT
                     AAAYSHVCDSPKHGEFVRRIEAMEDGEPLALGSPAFRVAIVPGAFYREHPHT"
     gene            complement(326..1048)
                     /locus_tag="VFB80_22165"
     CDS             complement(326..1048)
                     /locus_tag="VFB80_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN36558.1"
                     /translation="MNARHALVLALVTILPAAIVPLRGAFAQDEAKKPDAAAGAKTET
                     KAQDTAKEEVPAEDKPSHEALIALRDRLLKAYDAKDIDAVLAELDENVVVTWQDATVS
                     RGPDGVREYYDKKVGGPDSIVKDMKTTCTIDRYSQLYNDRRTAAAAGKLEQNFTLRDG
                     KQFQLTSPWTATLVKEEDEWKIAAFHVSTNMFDNGVLHLYVMQNRLWAGGIAGGVSLL
                     IGLLLGMAFARGGRKTPAGTGS"
     gene            complement(1045..2175)
                     /locus_tag="VFB80_22170"
     CDS             complement(1045..2175)
                     /locus_tag="VFB80_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN36559.1"
                     /translation="MPTTWWFSVINPMGVLQHPAARTRAGAPAAPSAGEFPAYPLSWY
                     LLGASREVRRDRPLAREIAGRRLVAFRTSRGTPVLLESRCCHLGADLSLGRICGDAIQ
                     CPFHHWEFGPDGACTKIPVRDDIPRWAQQTSYPVVERHGLVFFFNASEALFPLPFFFG
                     CRPDDFSRARPFATTLDCPWYLVGANAFDLQHFRAAHDRRLVGLPEVDCPAPFARRAT
                     GTFDVASDSLLDRLTRALAGSQVTMSITDYCGNLLFATAQFRRTCSYGMVCTTPLSSG
                     QTRVAVVVFVPRSASLAGRCLLDPLHAEVRRLFIKKFLSADAARLAGTRYSPSGLIAE
                     DQHLADYFLWLVQSSQGNAWAGQAAPPAVQSRPLSSVSEVSP"
     gene            2350..3528
                     /locus_tag="VFB80_22175"
     CDS             2350..3528
                     /locus_tag="VFB80_22175"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HZN36560.1"
                     /translation="MLPWVRNRLFREFHRRTLIYTACCEDPAVDRRALRLSPADRVLV
                     ITSGGCNALDYLLAGAGEVNAVDLNPCQSALLEFKAAAIRTLDYREFWELLGTGRSRQ
                     ARAMYGDAIRWQLSSAARRFWDRRISWFGGRDRWSSFYFRGGWGCVMRLIGGYWRTFR
                     GLEQPLADLFEARSLDEQRQIYERHLRGRVWTPWFSWLLSRPVCHALLGIPARQRQFI
                     LNYPGGLFRWGQALLDDLVANIPFATNYFMRANVLGAYAPDCCPEYLTPDGFSRLKSG
                     LLERLTIDTASVTEYLRQTRPGISKFALLDHLDWLEPQPLADEWEAIVAKANPGATVI
                     FRSALAHVDYLDGLGVRIDGRQAALGSLLVYDRQQAAALHASDRVHLYGSFSVARLPQ
                     "
     gene            complement(3575..>4001)
                     /locus_tag="VFB80_22180"
     CDS             complement(3575..>4001)
                     /locus_tag="VFB80_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36561.1"
                     /translation="ELTSGDWEGIYADNSGYVREAVGGPTGGHWHILVEEAGDYEFTL
                     RRWPEQTKVALGDKYEPSAKSPGAKAKVQTVGFPTIARARLEIAGHHAAADAKPKDTG
                     ATIRVANLPAGKTTLKAWFSDSGGKDLCGAFFVTVVRKK"
BASE COUNT          657 a         1277 c         1345 g          722 t
ORIGIN      
        1 gtgtgcggat gttcgcgata aaacgctccc ggcacgatgg ccacgcgaaa cgccggcgat
       61 cccagcgcca gcggctcgcc gtcctccatg gcctcgatac ggcgcacgaa ttccccatgc
      121 ttgggtgaat cgcacacgtg gctataagcc gcggccgtgg cgaaatcgat tccctggtcg
      181 gcggcgagct gtgccagcgc ctcgacgttc aagtcgccca cgcccgcgag ccaatcggcc
      241 gtcgccgcga gcaatcggcc gtcgtcgcta gggatggtgg agagcatggc aacgtcatcc
      301 ggcgttttgg ccgcgccact agcgactaag agcccgtacc ggccggcgtt ttgcgcccgc
      361 cgcgcgcgaa agccattccc agcaaaaggc cgatcagcag gctgaccccg ccggcaatgc
      421 cgccggccca gaggcggttt tgcatgacgt acaaatgcag cacgccgtta tcgaacatgt
      481 tcgtggagac gtgaaaggcg gcgattttcc attcgtcttc ctccttgacg agcgtggccg
      541 tccacgggct ggtgagttga aactgcttgc cgtcgcggag cgtgaagttc tgctcgagct
      601 ttccggcggc cgcggccgtg cggcgatcgt tgtagagctg cgagtagcgg tcgatcgtgc
      661 acgtcgtctt catgtctttc acgatcgagt cgggaccgcc gactttcttg tcgtaatact
      721 cgcggacccc gtccggcccg cggctgaccg tcgcatcctg ccaggtcacg actacgtttt
      781 cgtcgagctc cgccagcacg gcgtcgatgt ccttggcgtc gtatgccttc agtagccgat
      841 cgcggagcgc aatcagcgct tcgtgcgagg gcttgtcttc cgccggcact tcctccttcg
      901 ccgtatcttg tgcctttgtc tccgtctttg ccccggccgc tgcgtcgggc ttttttgcct
      961 cgtcctgggc aaacgcacca cggaggggca cgatggccgc aggcaggatc gtgaccagag
     1021 ccaggacaag cgcgtgtcgt gcgttcatgg cgatacctcg ctgacggagg agagcggacg
     1081 agattggact gccggcggcg cggcctggcc ggcccaggcg ttgccttgcg acgattgcac
     1141 cagccacagg aagtaatccg ccaggtgctg gtcctcggca atcaggccgc tggggctgta
     1201 ccgcgtgccc gccagccggg cggcatcagc gctcaagaat ttcttgatga acagccggcg
     1261 cacctcggcg tgcagcgggt ccagcaggca gcggccggcc agcgacgcgc tccgcggcac
     1321 gaacacgacg accgctactc gtgtctgtcc gctggacagc ggcgtcgtac acaccatgcc
     1381 gtagctgcac gtgcggcgaa actgggccgt ggcgaaaagc agattgccgc agtagtcggt
     1441 aatcgacatc gtgacctgcg agcccgccag cgcgcgagtc aggcggtcga gcagggaatc
     1501 gctggcaacg tcgaaggtac cggtggcgcg ccgggcgaac ggggccgggc aatcgacctc
     1561 ggggagaccc accagccggc ggtcgtgggc tgcgcggaag tgctgtagat cgaacgcgtt
     1621 ggcccccacc aggtaccagg gacaatcgag cgtcgtggca aaaggtcgcg cccgcgagaa
     1681 gtcgtcgggc cggcagccga aaaagaacgg cagcggaaac agcgcttcac tggcattgaa
     1741 gaaaaagacc aggccgtggc gctcgacgac cgggtagctg gtttgctggg cccagcgggg
     1801 aatgtcgtcg cggaccggaa tcttcgtgca agcgccgtcc gggccgaatt cccaatgatg
     1861 aaaggggcac tggatggcat cgccgcagat ccgccccaga ctcaagtccg ctcccagatg
     1921 gcagcagcgg gactccagca gcacgggcgt gccgcgcgac gtgcggaagg ccactagccg
     1981 ccggccggca atctcgcgag ccaaaggccg gtcgcggcga acctcgcggg aggcgcccag
     2041 cagataccag gagagcggat acgcgggaaa ctcgcccgca ctaggtgcgg cgggtgcgcc
     2101 ggcacgagtc ctggcagcag gatgttggag cacgcccatg ggattgatga ccgaaaacca
     2161 ccacgtcgtc ggcatccggc gctcacgcga ccggaaatgt atgtctgccg gcgctgccgg
     2221 tcaactatcg gcaccagaga accgtgcgtt tcgattcggg cggccgaccg cggtgcgcgc
     2281 ttgtttcctg taccagcgac gtgtaatctt gccgcagatc aggtcaattt gtctcggcgg
     2341 aactcggtca tgctgccgtg ggttcgcaat cgattgttcc gagagtttca ccggcgcacg
     2401 ctgatctaca cggcctgctg cgaagacccc gccgtcgacc gacgggcgct gcggctgtcg
     2461 ccggccgatc gcgtgctggt gattaccagc ggcggctgca acgcgctcga ttatctgctg
     2521 gccggcgcgg gggaggtgaa cgccgtcgat ctgaacccct gccaaagcgc gctactggaa
     2581 ttcaaggccg ccgccattcg cacgctcgat tatcgcgagt tctgggagct gctgggcact
     2641 ggccgcagcc ggcaggcacg ggccatgtat ggcgatgcga tccgctggca actttctagc
     2701 gccgcgcgcc ggttttggga tcgacggatt agttggttcg gcgggcgcga ccgctggtcg
     2761 tcgttctatt ttcgcggcgg gtggggctgc gtgatgcggc tgatcggcgg ctactggcgc
     2821 acgtttcgtg ggctagagca gccgctggca gacttgtttg aggcgcggag cctggacgag
     2881 cagaggcaga tctatgagcg gcatctgcgg ggccgcgtct ggaccccctg gttttcttgg
     2941 ctgctgtcgc ggccggtctg ccacgccctg ctgggaatac cggctcgcca gcggcaattc
     3001 atcctgaact atcccggcgg cctgtttcgc tggggccagg cgctgctcga cgacctggtg
     3061 gccaacatcc cgtttgccac caactacttt atgcgggcca acgtgctggg ggcttatgct
     3121 cccgattgct gccccgaata tctcacgccc gacggatttt cgcggctgaa aagcggcttg
     3181 ctcgagcggc tcacgatcga cacggccagc gtgacggagt acctgcggca aacgcggccc
     3241 gggatctcga agttcgcgct cttggatcat ctggactggc tggagccgca gccgctggcc
     3301 gatgaatggg aggcgatcgt ggccaaggcg aaccctggag cgacagtcat ctttcgcagc
     3361 gcacttgcgc acgtggatta cctggacggg cttggcgtgc gcattgacgg ccggcaagcc
     3421 gcgcttggct cgctccttgt gtatgatcgc cagcaagcgg cagcgctgca cgcgagcgac
     3481 cgcgtccatt tgtacggcag tttttcggtg gccaggctac cgcagtagcc ttcagcggct
     3541 aacagcgccc gttgcaagcg ccaacgctgg gccgctactt cttccgcacc accgtcacga
     3601 agaacgcgcc gcacaagtct ttgccacccg aatcgctgaa ccaggctttg agcgtggtct
     3661 tgcccgccgg gagattcgcc acgcggatgg tcgcgcccgt gtccttgggt ttggcgtcgg
     3721 ccgccgcgtg gtggccggcg atttcgagcc gagcgcgggc gatggtgggg aagccgacgg
     3781 tctgcacctt ggccttggcg ccgggggatt tggctgacgg ctcgtatttg tccccaagcg
     3841 cgactttggt ctgctccggc cagcgacgga gggtgaattc gtagtcgccg gcctcctcga
     3901 ccagaatgtg ccagtggccg ccggtcgggc cgccgaccgc ctcgcgaacg tagccgctgt
     3961 tgtcggcgta gatcccttcc caatcgccgc tggtcagctc g
//
LOCUS       DASRJB010000843         6455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1375577, whole genome shotgun sequence.
ACCESSION   DASRJB010000843 DASRJB010000000
VERSION     DASRJB010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375577"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>6455
                     /locus_tag="VFB80_22185"
     CDS             17..>6455
                     /locus_tag="VFB80_22185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36562.1"
                     /translation="MNERELFIATLQLDESRRADYLNKACTGDASMRQRVEALLAAFG
                     QAGSFLQPSAPEPSPTIDGPSERSGMQIGPYKLVEQIGEGGMGTVWKAQQSEPVKRLV
                     AIKLIKAGMDSRQVIARFDAERQALALMDHANIARVLDAGTTPAGRPYFVMDLVRGVP
                     ITRYCDEHHLTPRERLELFIPVCQAVQHAHQKGVIHRDIKPSNVLVALYDGRPVPKVI
                     DFGVAKAAGQSLTDKTLVTGFGTIVGTLEYMSPEQAETNQLDIDTRSDIYSLGVLLYE
                     LLTGSTPFSRKELENAGMLEMLRVIREEEPSKPSTKLSTAEGLPTLAANRGTEPGRLT
                     RLVRGELDWIVMKALEKDRNRRYETANGFAMDVQRYLADEHVQACPPSAGYRLRKFAR
                     RYKGALATTAIVAAALVLGSVVSVWQAVRATNALQAEKKARRDAEIATEGEKTQRGVA
                     QLQKTLAEKNATRAEANFGKARKAVEEYLTKITENELLRVPGLQPLREDLLKAALKFY
                     TEFTQERAGDPTLRQELASAHLLLARIQGELDNSKAARAANLESIRLYEQLRADAERG
                     KSEASVEVQVGLATGYFLAGRYDDTEKLCQHVLQIAPKHAEVRGLLSDTYNELGKAAN
                     DKNDVPATFKHYQRAFDLREGLVRDFPDNAAYLARLGGTLNNLGNLLSNQKQDEEALA
                     MYERSIDYTAQAYEREPHSIVWGRWLCIQLGNAASKYDNLDRRDQALATLERLVAVSR
                     KRSFENPAVSSLRGDLYKAYLRLGTYQKEVGDAAGASKNFRLAREVLEQIPRETPKDL
                     FELARVYAVLASPPADAASSSENQSDADERQRNADLAIATLQKAVAGGFQDPAELAKY
                     YNDFDSLRGRSDFQEIAATVQKLAEANQKLAQASRQDPATQLASQQQAAALLKDLATA
                     QPGQERHRTTLATTLQSIGMVQTGLKQYEAAEKSMNDAIALWRELAAAQPNNPQLQVS
                     LIGAQMALGACYRESGRPAEAHRVWQECLASLDQVRQRQAADTTLGKQIALKEAQICD
                     LYGRLGAFSLAAEYSRRSVRLQRSLGAWPDVDYGVVLGVLQGDEALRELCRVVDRERT
                     RAGGPPFTDGDLANLGKTSNLLNPPPLPPEQSLKWIEEAAKANPDSWRLYLLALAQHR
                     AGKSADAQATFEKVRTSKDLSTVFAGALIAHGAGQPDLARERLMMAEAIFVQFCQQTL
                     QGSTVGLASPISQSGHWWALTAPVLLRREAWRLIDGQTVPEEAWLRLMEARAAHLLGD
                     SAKRDEQLAAADSRDSNVWLARARLTEEWGQLDQSAEVAWQRAIDAAPADPVPWIHRG
                     RWCIQRGEQVKADADFAKAAALSPDTLNRFLQAGWWVAGPYPKGLKEFCPPEVDPDPS
                     RPVHLVDPKTGLSEQPLAWRPVPSGETGKIDLKATFNADQISAYGLNIVYSPAERPAT
                     LRLSGDGGLRLWLNGELVYEADPAPTWQWANEGLVPVTLRAGRNLVMVKVSQSTQKHS
                     FSLRVSDEAVDRAFLLAEWGLWQEAAEQMALAAPSDFREDSLAVRHAELLLAANDLDG
                     YRRYREWMLQQIARLGTGPASSFARTCALVPAESSDAARLIQLAEKGLDPKSQNSIGV
                     LGYALYRGGRYDEAIARLREVTGHSLHDLVLAMALWRRGDQDAARQLLVKSQTAYDQE
                     TRKALAASAYKRPPWNWRDFACFQILYREARALIEGGAAAGDENAASLQARARAEFVR
                     RDPLTADFDQALMVEPKQPRHWLARGRRLAELGRFDEALADFNKAVELGANDPEVLAA
                     RALHFADHGEAAKAAADFHAALRLFDDTGAKPRWYFGVPIDFEVAQRDDVLEQLRALR
                     PLDPRPLLTRMVLSMQRGDIGAAKSAAQSLAAYGYAQPGWLAAAARWRGDDAEFARLR
                     ASSADPNPNAQIMLIGLAPTDGALTAKIQTAGERMWREQPQDRWHRRWLGLAQLRAGQ
                     WTEAAASLETSLDPGDRWEQDRLVWPLLAIAHHQLKNAEPARRWLNRTEHGMELGAQA
                     GPGELAAGTAIPGMLPEEWLYGMLFYLEAKTLIDGPDAARTERERLAALARGRSQVIL
                     AKADAARQQSLAKVEAARQESLAKAEAAWNRAVELAGDNPLPWIQRGRWYLERGEQAK
                     ADADFAKAATLTPD"
BASE COUNT         1222 a         2058 c         2159 g         1016 t
ORIGIN      
        1 cccttgaggt cccgccgtga acgagcgtga acttttcatc gccactctgc agctcgacga
       61 aagccggcgc gcagactact tgaacaaggc ctgcaccggc gatgcctcca tgcgccagcg
      121 cgtcgaggcc ttgctggcgg ccttcggcca ggccggcagc tttcttcagc cgtcggcgcc
      181 ggaacccagc cccacaatcg acggcccgtc ggaacgatcc ggcatgcaga tcggccccta
      241 caagctggtc gagcaaatcg gcgaaggggg catgggcacg gtctggaagg cccagcagtc
      301 cgagccggtc aagcggctgg tggcgatcaa gctcatcaag gcggggatgg actcgcggca
      361 ggtgatcgcc cgcttcgacg ccgagcgcca ggcgctggcc ctgatggatc acgccaacat
      421 cgcccgcgtg ctggacgccg gtacgacgcc cgccggccgg ccgtatttcg tgatggacct
      481 ggttcgcggc gtaccgatca cccgctattg cgacgagcat catctcacgc cccgcgagcg
      541 gctggagctg ttcatcccgg tttgccaggc agtccagcac gcccatcaaa agggcgtgat
      601 ccatcgggat atcaagccgt ccaacgtgct tgtcgccctg tacgacggca ggccggtgcc
      661 caaagtgatc gactttggcg tggccaaggc ggccgggcag tcgctcaccg acaaaacgct
      721 cgtcaccggc tttggcacga tcgtcggcac gctggaatac atgtcgccgg agcaggccga
      781 gaccaaccag ctcgacatcg acacgcggag cgacatctac tcgctgggcg tactgctcta
      841 cgagctattg accggcagca ctccgttcag ccgcaaggag ctcgaaaatg ccggcatgct
      901 ggagatgctg cgggtgattc gcgaggagga gccctccaag ccgagtacca agctcagcac
      961 ggcggagggc ttgcccacgc tggccgccaa ccgcggcacc gagccgggca ggttgacgag
     1021 gctggtccgc ggcgagctcg actggatcgt gatgaaggcc ctggaaaagg accgcaaccg
     1081 ccgctacgaa acggccaacg gctttgccat ggatgtgcag cggtacctgg ctgatgagca
     1141 cgtgcaggcc tgcccgccgt cggccggata ccggctgcgg aaattcgcgc ggcggtacaa
     1201 gggcgcactg gctacgacgg cgatcgtggc agcggcactc gtgctgggca gcgtggttag
     1261 cgtctggcag gcggttcgcg cgaccaatgc cctgcaggcc gaaaagaaag cacgccgcga
     1321 cgcggaaata gcgacagagg gcgagaaaac gcaacgggga gtggcccagc tgcagaagac
     1381 gttggccgag aagaacgcca ctcgagcgga agccaacttt ggcaaggccc gcaaggcggt
     1441 cgaggagtac ctgacgaaga tcaccgaaaa cgagctgctg cgagtccccg ggttgcagcc
     1501 gctgcgcgaa gatttgctga aggccgccct caagttttac accgagttca cgcaggagcg
     1561 ggccggcgac ccgacgctgc ggcaggagtt ggcctcggcg cacctgctgc tcgccagaat
     1621 tcagggcgag ctcgacaata gcaaggcggc gcgggccgcg aacctggagt cgatccgcct
     1681 gtacgagcaa cttcgggccg atgccgagcg cggcaaaagt gaggctagtg tcgaagtaca
     1741 ggtggggttg gccaccgggt atttcttggc tggccgctat gacgacaccg agaaactgtg
     1801 tcagcacgtc ctgcagatcg caccgaagca tgccgaagtc cgcggcttgt tatcggacac
     1861 ctataacgag ttgggcaaag cggccaatga caagaacgat gtcccggcga ccttcaagca
     1921 ttaccagcgg gcgttcgact tgcgtgaagg gctggtccgc gattttccgg ataacgccgc
     1981 ctatttagcc cgcctcggcg gaacgctcaa taacctgggc aacctgctaa gcaatcagaa
     2041 acaggacgaa gaagccctgg ccatgtacga gcgttcgatc gactacaccg ctcaggccta
     2101 tgagcgggag ccgcactcga ttgtgtgggg ccgatggctg tgcattcaac tcggcaatgc
     2161 ggccagcaaa tatgacaatc tcgacaggcg cgaccaggcg ctggcgactc tcgagcgcct
     2221 ggtggccgtc agtcgcaagc ggtcgttcga gaaccccgcc gtcagctctc tgcgtggcga
     2281 cttgtataag gcttacttga ggctggggac gtatcagaag gaggtgggag acgccgctgg
     2341 agcgagcaag aattttcgtc tggctcgcga agtgctcgag caaattcctc gcgagacgcc
     2401 aaaggatcta tttgagctgg ctagggtgta tgccgtgttg gcgagcccgc cggctgacgc
     2461 ggctagctcc agcgaaaacc aaagcgatgc cgacgaacgc cagcggaacg ccgacctggc
     2521 gatcgccacg ctccaaaagg ccgtcgctgg cggctttcaa gacccagccg agctcgcgaa
     2581 gtactacaac gacttcgact ccctgcgcgg ccgcagcgac tttcaggaaa ttgcggccac
     2641 cgtccagaag ctcgccgagg ccaaccagaa gctggcccag gcctcccggc aggaccccgc
     2701 cacacagctc gccagccagc agcaggcggc tgcgctattg aaggaccttg ccaccgcgca
     2761 gccgggccag gagcggcatc gcacgacttt ggccacgacc ttgcaatcga tcggcatggt
     2821 gcaaaccggg ctcaagcaat atgaggcggc cgaaaagtca atgaacgacg cgatcgcgtt
     2881 atggcgcgaa ttggcagccg cgcagccgaa caatccccaa ctgcaagtca gcctgatcgg
     2941 cgcgcagatg gcattgggcg cgtgttatcg cgagagcggg cggccagcgg aggcgcatcg
     3001 cgtctggcag gagtgcctcg ccagcttgga tcaagttcgc cagcgtcagg ccgcagacac
     3061 aacgctgggc aaacagatcg ccctgaagga agctcagatc tgcgatctct acggtcgcct
     3121 gggagccttc tcgctggccg ccgagtattc gcgccggagc gttcgcttgc agcggtccct
     3181 cggcgcctgg cccgacgtcg attacggcgt tgtcttggga gtgctgcaag gagacgaagc
     3241 gctccgcgag ctgtgccgtg tcgtcgatcg ggagcggacc agagcgggcg gcccgccatt
     3301 taccgacggt gacttggcga acctgggtaa gaccagcaac ctgctgaacc cgccgcccct
     3361 gccgccggag caatcgctga agtggatcga ggaggcggcc aaggcgaacc ccgactcgtg
     3421 gcgcctctac ctgctcgccc tggcgcaaca tcgagccgga aagtcggccg acgcgcaggc
     3481 cacgtttgag aaggtgagga cgtccaagga cctttctacg gtctttgccg gcgccctgat
     3541 cgcccacggc gcaggccagc ccgacctggc acgcgagcgg ctgatgatgg cggaagccat
     3601 cttcgtccaa ttctgccagc agacgctgca agggagcacg gttggactgg cgtcgcccat
     3661 cagtcagtcc ggtcattggt gggcgctgac ggcccccgtt ctgctgcgcc gcgaagcttg
     3721 gcgcttgatc gatggtcaga ccgtccccga ggaggcctgg ctgcggttga tggaagctcg
     3781 cgccgcccat ttgctgggcg acagcgctaa gcgggacgaa caactcgcag ccgccgatag
     3841 ccgcgactcc aatgtgtggc tggctcgcgc gcggcttacc gaagagtggg gccagctcga
     3901 ccagtcggcc gaagtcgcct ggcagcgcgc catcgatgcc gccccggccg acccagttcc
     3961 ctggattcac cgcggccgct ggtgcatcca gcgtggcgag caagtcaagg ccgacgccga
     4021 ctttgccaag gccgccgcgc tgtcgcccga tactctcaac agattcctgc aagccggctg
     4081 gtgggtggcc gggccgtatc ccaagggact gaaggaattt tgcccgcctg aggtcgatcc
     4141 cgatccgtcg cggcccgtgc acttggtcga tcccaaaacc gggctgagcg agcagcccct
     4201 tgcctggcgg cctgttccca gtggcgaaac gggaaaaatc gatctgaagg cgacgttcaa
     4261 cgccgatcag atttcagcct atggcctgaa catcgtctat tcgcccgcgg agcgcccggc
     4321 aacgttgcgg ctgtcgggcg acggcggcct gcggctgtgg ctgaacggcg aactggtgta
     4381 cgaggcggac ccggcgccca cctggcagtg ggcgaatgag ggtctggttc ccgtcacgct
     4441 tcgcgccggt cgcaacctgg tgatggtaaa ggtcagccag agcacccaaa agcactcgtt
     4501 cagcctcagg gtgtccgacg aggccgtcga tcgcgcattc ctcctggccg agtggggact
     4561 gtggcaggaa gccgcggagc aaatggccct ggcagctccg agcgactttc gcgaagactc
     4621 gctcgccgtg cgtcatgccg aattgttgct ggccgcgaac gatctggacg gttaccggcg
     4681 ttaccgcgaa tggatgctgc agcaaattgc caggctggga acagggcccg ccagcagctt
     4741 cgccaggacc tgtgcgctcg tgcccgccga gtcctccgat gcggcgcggt tgattcaact
     4801 ggccgagaag ggtctcgatc ccaagagtca gaacagcatc ggcgtcctgg gctatgccct
     4861 gtatcggggc gggcgctacg acgaggccat tgcgcgtctg cgcgaggtta ccggccattc
     4921 cctgcatgat ctcgtgctgg cgatggcgct ctggcggcgc ggcgatcagg atgctgcccg
     4981 ccagttgctg gtgaagtccc agaccgcgta cgaccaggag acccgcaagg ctctggccgc
     5041 gtcagcgtac aagcggccgc cttggaattg gcgcgatttt gcctgcttcc agattctcta
     5101 tcgcgaagcg cgggccctga tcgagggcgg cgcagcagct ggcgacgaga acgccgcgag
     5161 cctgcaagcc cgcgcccgcg cagaattcgt acgccgcgat ccgctaacgg ctgactttga
     5221 ccaggctctg atggtcgagc ctaagcagcc gcggcactgg ttggcccgtg gccgccggct
     5281 ggccgagctg ggccggttcg acgaggccct ggccgatttc aacaaggccg tggagctcgg
     5341 tgcaaacgat ccagaggtgc ttgcggcgcg ggcgctgcac tttgccgacc atggcgaggc
     5401 tgccaaggcc gcagccgact ttcacgcggc gctaagacta ttcgacgata ccggggccaa
     5461 gccgcggtgg tactttggcg tgccgatcga tttcgaggtc gcccagcggg acgacgtact
     5521 tgagcaactg agggctctcc gtccgctgga cccgcggccg ctgttgaccc ggatggtcct
     5581 cagcatgcag cggggggata ttggcgcagc gaagtcggcc gcacagagtt tggcagccta
     5641 cggctatgca caacccggtt ggctggcggc agcggcccgc tggcgaggag acgacgccga
     5701 gttcgcccgc ctcagggcca gcagcgccga tcccaatccg aacgcgcaga ttatgctgat
     5761 cggcttggcg cccacggatg gagcgcttac cgccaagatc caaacggctg gcgagaggat
     5821 gtggcgtgag cagccccagg atcgttggca ccggcggtgg ctgggcctgg ctcaattgcg
     5881 cgccggtcaa tggaccgagg cggctgcgag cctggaaacg tcgctcgatc cgggcgaccg
     5941 ttgggagcag gatcgcctgg tctggccact gctggccatc gcgcatcacc aattgaaaaa
     6001 cgccgagccc gcccgccgct ggctcaaccg gaccgagcac ggaatggaat tgggagccca
     6061 ggcggggccc ggggaacttg cagccggtac agcgattcca ggcatgctgc cggaagaatg
     6121 gctctatggg atgctctttt atctcgaagc caagaccctg atcgacgggc cggatgcggc
     6181 ccgcaccgag cgcgagcgcc tggcggcact ggctcgcggc cggagccagg tcattctggc
     6241 aaaggccgac gccgcgaggc agcagagcct ggcgaaagtc gaggctgccc ggcaggagag
     6301 cctggcgaag gccgaggccg cctggaaccg cgcggtcgag ctggccggcg acaatccact
     6361 gccctggatc cagcgcggcc gctggtacct cgagcgcggc gagcaggcca aggcagacgc
     6421 ggacttcgcc aaggccgcca cactgacacc tgacg
//
LOCUS       DASRJB010000844         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3913821, whole genome shotgun sequence.
ACCESSION   DASRJB010000844 DASRJB010000000
VERSION     DASRJB010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3913821"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFB80_22190"
     CDS             <1..565
                     /locus_tag="VFB80_22190"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZN36563.1"
                     /translation="AAKERNTLEVRSLQAWRKWLAANHETQAEVWLVFFKRHTGKECL
                     SYQDALDEALCFGWVDSLVKRLDNDRYARKFTPRTADSRWSAINRRRYAELKAAGRLA
                     APGVARAPTKGSAYPPRPPKSTVPTYIEARLKANSRAWEFFEQLAPSYRQLYVLWIDS
                     AKRQETKEKRLREAIAMLAAGQKLGLK"
     gene            802..2058
                     /locus_tag="VFB80_22195"
     CDS             802..2058
                     /locus_tag="VFB80_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36564.1"
                     /translation="MYRTAALVVALLGILCPSMPAAAAEAPLVEKYLTEGRLAVGEKE
                     LQQHLRNHPDDDQARFGLGTLQFLKAVERLGQSLHRYGALGPQSRLARAIPLVRLPVP
                     QNPKPEPIGYEDSRKMVEQWLADMQAVEQTLAPIKDREVKLPLHFGLIALDLNGDGQA
                     GGDETLWRIYAVLNNGLRLSPEFTPEEVQNFVIAFDYGDVHWLRGYCRLLSAIGEMTL
                     AYDQRSVFDAIAHQLFDNPKGAAIPEELFPRREDRPFESEIADFIAAIHLARFPLKEP
                     QRMQAARKHLLAVVEHSRESWKAIAAETDDDREWIPSSKQKGVIPNVQVSAEMIQGWQ
                     SFLGEAEQLLEGKKLVPHWRLVEGRGVNLKRVFDEPREFDLVMWVHGAAAVPYVEAGP
                     TTQLETWTRLNALFGGQFIGFAFWFN"
     gene            complement(2300..3400)
                     /locus_tag="VFB80_22200"
     CDS             complement(2300..3400)
                     /locus_tag="VFB80_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZN36565.1"
                     /translation="MSFTVLRGRWAAGGMLALVAGLAAAWAAAACAQTPPAAAPGVVG
                     SEFIYETAPFPQCHASTIAQTKTGLVCAWFGGMRERAPDVGIWVSRHDGKAWSPPVEV
                     ANGVQDATTRHPCWNPVLFQMPDGPLLLFYKVGPSPSTWWGMRMQSADGGKTWTKPVK
                     LPDDIPGPIKNKPVLLADGRLLSGSSSEDQGWRVHMEWTKDAGQTWERTPVLCDGKTQ
                     NAIQPTIFQHGDQLQIVCRNRQAGSLWQSWSSDGGRTWSKFEALNLPHPGSGIDGVTL
                     KDGRHLLVYNHTNRGRSPLNVAVSKDGKTWQAALVLESEPGEYSYPAAIQTADGLVHI
                     TYTWKRQRVRHVTLDPSKLVLKPIVDGQWPAP"
     gene            3519..>4084
                     /locus_tag="VFB80_22205"
     CDS             3519..>4084
                     /locus_tag="VFB80_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZN36566.1"
                     /translation="MASGKYLFTSESVSMGHPDKLADQISDGVLDALLAQDPKSRVAC
                     ETLVTTDYCVLAGEITTTAKVDYEKVARDVITAVGYTHDDIGFNAKTCKVDVKLHSQS
                     PDIAMGVDKEGAGDQGLMFGYACDETPELMPLPIALAHRILNRLTRARQSGEVDWLRP
                     DSKSQVTVEYDGDRAVRIDAVVVSTQHSP"
BASE COUNT          726 a         1272 c         1361 g          725 t
ORIGIN      
        1 ggctgccaag gaacgcaata cgctggaggt gcgcagtttg caggcctggc ggaagtggct
       61 cgccgccaat cacgagacgc aggccgaggt ctggctggtg ttcttcaagc ggcatacggg
      121 gaaggagtgc ctcagctacc aggacgcgct cgatgaagcg ctctgcttcg gctgggtgga
      181 cagcctcgtg aagcggctgg acaacgaccg ctacgcccgc aagttcacgc cacgcacggc
      241 ggacagccgg tggtcggcca tcaatcggcg gcgttacgca gagctgaagg cggccggccg
      301 gctggcagct ccgggtgtgg cccgagcgcc gacgaagggc agcgcctatc ccccgcggcc
      361 acccaaatcc acggttccga cctacatcga agcccgcttg aaagccaaca gccgggcgtg
      421 ggagttcttc gagcaattag cgccgtccta ccggcagctg tatgtcctct ggatcgattc
      481 ggccaagcgc caggaaacca aggagaagcg gctccgcgag gcgattgcga tgctggccgc
      541 cggccagaag cttgggctga aatagtcagt cggctagcgg ccttaacggc acgcgaagtg
      601 cccgccgcat ttcgggcgcg ccttttgccg gcgtattttg tgcctgtgcc agtgggatgc
      661 tcgtttttgt aggtcccgcc tgccgggcgg gacctgggcc gccgcggtac gtcgaatcct
      721 cggcagtgtt ccgggtcgct gcgcttccct ttagacccct tccggcagaa ggggcctact
      781 tcatcgctag gagcccgtgt catgtaccgt accgctgcgc tggtggttgc ccttctggga
      841 atcttgtgtc catcgatgcc cgccgcggca gccgaagcgc cgctggtcga gaaatatctc
      901 accgaaggcc gcctggcagt cggcgaaaag gaattgcagc agcatttgag aaaccatccc
      961 gacgacgacc aggcccgctt cgggttgggt acgctgcagt tcctgaaggc cgtggagcgg
     1021 ctgggacagt cgctgcaccg ctatggagcc ctcggaccgc agagccgcct ggcccgggcg
     1081 attccgctgg tgcggctgcc cgtgccgcag aatcccaaac ccgagccgat cggctacgag
     1141 gactcgcgaa aaatggtgga gcagtggctg gccgacatgc aggccgtgga gcagacgctc
     1201 gcgccgatca aggaccggga agtcaagctg ccgctgcact tcggcctgat tgccctcgac
     1261 ctgaacggcg acggccaggc gggcggcgac gaaaccctgt ggcggatcta cgcggtcctc
     1321 aacaacgggt tgcggctcag cccggaattc acgcccgagg aagtgcagaa cttcgtcatc
     1381 gccttcgatt acggcgacgt gcattggctc cgcggctatt gccggctgct ctcggcgatc
     1441 ggcgaaatga ccctggccta cgaccagcgg agcgttttcg acgccatcgc tcaccagttg
     1501 ttcgacaatc cgaagggggc cgcgattccc gaggagctct ttccgcgccg cgaggaccgg
     1561 ccgttcgaga gtgaaatcgc cgatttcatt gccgccattc acctggctcg cttcccgcta
     1621 aaggagcccc agcggatgca ggcggcccgc aagcacttgc tggccgtcgt ggagcacagc
     1681 cgcgagagct ggaaagcgat cgccgccgag accgacgatg accgcgagtg gattccgagc
     1741 agcaagcaga agggggtcat tccgaacgtg caagtctcag ccgagatgat ccagggctgg
     1801 cagtccttcc tgggcgaggc cgagcagctg ctggaaggga agaaactcgt gccgcactgg
     1861 cgcttggttg aaggccgcgg ggtgaacctg aagcgggtgt ttgacgagcc gcgggagttc
     1921 gacctcgtga tgtgggtcca cggcgcagca gccgtgccct atgtcgaagc cggcccgacg
     1981 acgcagctcg aaacctggac gcgactcaac gccctgttcg gcggccagtt catcggcttc
     2041 gcgttctggt tcaactgagt gctgcataca cgactttttg acaaatagtg gacaagtggc
     2101 acgatttttg cagcaaggtt cccgatgcgc ggcagtctgc caaattggcc gcgagcggct
     2161 cgccggacgg gactggaaag tcgttctcaa actctaataa gtactactta aggggttgcc
     2221 gcgatttcga cgcaagtcat tgagtggtca tctcttgcgg cgaaacaggc gcgtgaacga
     2281 tccaccttac cgggggccct catggcgcag gccactggcc gtccacgatg ggcttgagca
     2341 cgagcttgga cgggtccagc gtgacgtgcc tcacgcgctg ccgcttccag gtgtaggtga
     2401 tatggacgag cccgtcggcc gtttggatgg ccgcgggata ggagtactcg ccgggctcgc
     2461 tttccagcac gagggcggct tgccaggtct tgccgtcctt gctgacggcg acgttcagcg
     2521 gcgagcggcc gcggttggtg tggttataga ccagcaggtg ccggccgtcc ttgagcgtca
     2581 cgccgtcgat acccgagccg ggatgcggca ggttcagcgc ctcgaacttg ctccaggtgc
     2641 ggccgccgtc gctcgaccag gattgccaga gcgagccggc ctgccggttg cggcagacaa
     2701 tttggagttg atcgccgtgt tgaaagatcg tgggctgaat cgcgttttgc gttttgccgt
     2761 cgcagaggac gggcgttcgc tcccaggtct ggcccgcgtc cttggtccat tccatgtgga
     2821 cgcgccagcc ctggtcctcg ctcgagctgc cgctgagcag ccggccgtcg gccagcagca
     2881 ctggcttgtt cttgatcggg ccggggatgt cgtccggcag tttcactggt ttcgtccagg
     2941 ttttgccgcc gtcagccgac tgcatccgca tgccccacca ggtgctcggg ctgggaccga
     3001 ccttgtagaa caagagcagc gggccatcgg gcatctgaaa cagcaccgga ttccagcagg
     3061 gatgcctcgt ggtcgcgtcc tgcacgccgt tggcgacttc gaccggcggc gaccaggcct
     3121 tgccgtcgtg ccggctgacc caaatgccga cgtccggcgc gcgttcgcgc atgccgccga
     3181 accaggcgca gaccaggccc gttttggtct gtgcgatcgt cgaggcgtgg cattgcggga
     3241 agggcgcggt ttcatagatg aattcgctgc cgaccacgcc gggagcagca gccgggggtg
     3301 tctgggcgca ggcggccgcg gcccaagcgg ccgccaatcc ggcgacaagg gccagcattc
     3361 cgcccgcagc ccagcgccct cgcagtaccg tgaaagacat cggttttccc ctattcggcg
     3421 gttcaattag cggttgtgaa atcgcgggca gcgaccttat aatcgccgct ggttattccg
     3481 gtcagcagtt tcgcggtttt gattgtcagg agcaacctgt ggcaagcggc aagtatttgt
     3541 tcacgagcga atcggtcagc atgggacacc cggacaagct ggccgaccag atttccgacg
     3601 gcgtactgga cgccctgctg gcccaggacc ccaagagccg cgtggcctgc gaaacgctgg
     3661 tgaccaccga ttactgcgtg ctggcgggcg agatcacgac gacggccaag gtcgattacg
     3721 agaaagtcgc ccgcgacgtc attaccgcgg tcggctacac gcacgacgac atcggcttca
     3781 acgccaagac gtgcaaagtc gatgtgaagc tgcacagcca gagccccgac atcgccatgg
     3841 gggtcgataa ggaaggcgcc ggcgaccagg gcctgatgtt cggctatgcc tgcgacgaga
     3901 cgcccgagct gatgccgctg ccgatcgccc tggctcaccg gattctcaac cggctaacgc
     3961 gggcgcgaca gtcgggcgaa gtcgattggc tgcggcccga cagcaagagc caggtcacgg
     4021 tcgaatacga cggcgaccgg gccgtgcgga tcgacgccgt ggtcgtcagc acgcagcatt
     4081 cgcc
//
LOCUS       DASRJB010000845         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_540514, whole genome shotgun sequence.
ACCESSION   DASRJB010000845 DASRJB010000000
VERSION     DASRJB010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540514"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFB80_22210"
     CDS             <1..1136
                     /locus_tag="VFB80_22210"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZN36567.1"
                     /translation="TVRELATDVPTGPPWLESLLTTAARIVAGPLSTLLLIVLWFTAF
                     ARIRRELTPFLISRPIVSGSGMIDDQGSFHLSDKAPAMSCLTGLGGWLGSRPIYCLVN
                     FLKPVFAESRLVWQQYRRLFAPRQRLQIALGDSNLADAAEYLRVGTTMLVLDVIEAGE
                     MPSVPRVRRPIRSLRAICSDPTLAARIPLAGGGSATALEIQRFYLEACRRFLERRPDA
                     PAEARDVLRRWEDVLDMLTDDPQGLVGTLDWVTKQFVLDKAGRGAAWEVRKKIDIRYH
                     ELSPQGYFQRLRTTGIVLDILERAEIDHARRNPPLGTPATVRGRYIREWAADEELISA
                     NWHAVYLGQGRECKVVRLDQVHARPAEQAADQRKRSPRNSDRN"
     gene            complement(1235..>3508)
                     /locus_tag="VFB80_22215"
     CDS             complement(1235..>3508)
                     /locus_tag="VFB80_22215"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN36568.1"
                     /translation="ISLMFRRATILFLITAAASAAEPVRKPYGIERREQWTTGNLHGT
                     PEPPDPYRTERVFPKLELFEPLSVGVVPGQNRFGVATRPGKIYSFEIRPDVDKADLLI
                     DVKRTTYGLAFHPKFAENGYFYVTSLIDPPPAPDRGSRLSRYKVNPGQPLAADPASET
                     VILEWPAGGHNGGCIRFGPDGYLYLATGDGSGIADELLTGQTIDDLLGAILRIDVDRP
                     ADGKNYSIPADNPFAGREKARPEIWSFGHRQIWKFSFDPPTRRLWAAEVGQDLWEMVY
                     LIRRGGNYGWSVREGNHPFRPTRPQGPAAFELPVVEQPHSDFRSITAGYVYGSDRLPE
                     LKGAFLYGDYDTGRLWLLRYEGDRGGTGKVVEHRQLADTQVRPVEFALDPSGEVYLVD
                     FAGGGFHRLVKAPPAVETKPFPRKLSETGLFASTKDNVPAKGLIPYSVNAPLWSDHAE
                     KERFLALPGDSQIEFDDVVYPHGPAYSDLGWRFPDGAVLVKTFSLGSRRLETRILQHR
                     KMPGNDDEYGAQYWFGYTYVWNDEQTDAELLSPAGLDREFTVPDPASPGGVRRQTWHF
                     PSRAECTLCHTMAAKYALGVTTLQMNKDHDYGGTLANQLATLEHLGVFKEKLPKRPEE
                     LPSLVDYRDASQDRHLRARAYLHANCAHCHRKWGGGNAEFELQASIPLAATKAVDTPP
                     GQGAFNLRDPKIIAPGDPDRSLILHRLQLTTLGRMPHVATSVVDRQAAQLIREWLTDL
                     RDPELLKKPGAIHPRLP"
BASE COUNT          549 a         1148 c         1224 g          587 t
ORIGIN      
        1 gcacggttcg cgagctggcg accgacgtgc cgacaggccc gccctggctg gagtcgctgc
       61 tcacgaccgc ggcgcgaatc gtcgccggcc cgctttcgac gctgctcttg atcgtgctct
      121 ggttcacggc ctttgcccgg attcgccgcg agctgacgcc gtttctgatc agccggccga
      181 tcgtcagcgg cagcgggatg atcgacgatc aggggagttt ccacctgtcc gacaaagcgc
      241 cggcgatgag ctgcctgacc ggcctggggg gctggctcgg ctcgcggccg atctactgcc
      301 tggtcaattt cctgaagccg gtgtttgccg aatcgcggct ggtgtggcag caataccggc
      361 ggctgttcgc cccgcggcag cggctgcaaa ttgctctggg cgattcgaac ctggccgacg
      421 ccgccgagta cctgcgggtc ggcacgacca tgctggtgct ggacgtgatc gaggcgggcg
      481 agatgcccag cgtgccccgc gtgcggcggc cgatccgctc gctgcgggcc atctgcagcg
      541 atccgacgct ggccgcacgc attccgctgg ccggcggcgg gtcggccacg gcgctcgaaa
      601 tccagcggtt ctacctggag gcctgccgtc gcttcctgga gcgccggccc gacgcaccgg
      661 cggaagcccg cgacgtattg cgccgctggg aagacgtgct ggatatgctg accgacgatc
      721 cgcagggcct ggtgggcacg ctcgactggg tcaccaagca gttcgtcctc gacaaggccg
      781 gccgcggggc tgcctgggaa gtccgcaaga agatcgacat ccgctatcac gagctttcgc
      841 cccagggcta tttccagcgg ctgcggacga ccggcatcgt gctcgacatc ctggagcggg
      901 cggagatcga ccatgcccgc cgcaatccgc cgttgggtac gccggccacg gtccgcggcc
      961 gctatatccg cgagtgggcg gccgacgaag agctgatttc tgcgaactgg catgctgtct
     1021 atctcggcca gggccgcgaa tgcaaagtcg tccggctcga ccaggtccat gcccgccccg
     1081 ccgagcaagc cgccgaccag cgcaagcgct cgccgcggaa ctcggatcgg aattagtagg
     1141 gtgggtcaag cgagtccgcg agcgcagacc cacgcggaag cgtgtgcagg cgctgacccg
     1201 cgtgggtctt cggccgcagc ggcctcgacc caccctacgg cagccgcgga tgaatcgcac
     1261 cgggcttctt cagcagctcg ggatcgcgga ggtcggtcag ccactcgcga atgagctggg
     1321 ccgcctggcg atcgacgacg ctggtagcga cgtggggcat gcggccgagc gtggtgagct
     1381 gcaggcgatg gaggatgagc gagcgatccg gatcgcccgg cgcgatgatc ttgggatcgc
     1441 gcaggttgaa cgcgccctga cccggcgggg tatcgacggc cttggtggcc gcgagcggga
     1501 tcgaggcctg gagctcgaac tcggcgttgc cgccgcccca tttgcggtgg cagtgggcgc
     1561 agttggcgtg caggtaggcc cgcgcccgca ggtgccggtc ctgcgaggcg tcgcgatagt
     1621 ccaccaggct cggcagctcc tccggccgct tgggcagctt ctccttgaag acgcctagat
     1681 gctccagcgt cgccagctgg ttggcgagcg tcccgccgta gtcgtggtcc ttgttcattt
     1741 gcagcgtcgt tacgccgagg gcgtatttgg ccgccatcgt gtggcagagc gtacactcgg
     1801 cccggctggg aaaatgccag gtctgcctcc gtacgccgcc gggcgaagcc gggtcgggca
     1861 cggtgaactc gcggtccagt ccagccggag agaggagctc ggcgtcggtc tgctcgtcgt
     1921 tccagacata cgtgtagccg aaccagtact gcgcgccgta ttcgtcgtcg ttaccgggca
     1981 tcttgcggtg ctggaggatt cgcgtctcca gccgccggct gccgagggaa aacgtcttca
     2041 ccagcactgc cccgtcggga aaccgccagc ccagatcgga atacgccggc ccgtgcggat
     2101 agaccacgtc gtcgaactcg atctgcgaat cgcccggcag cgcgaggaac cgctccttct
     2161 cggcatggtc cgaccagagc ggggcgttga ccgaataggg aatcagccct ttggcgggca
     2221 cgttgtcctt ggtcgaggca aacagccccg tctcgctgag cttgcgcggg aacggcttcg
     2281 tttcgactgc cggaggtgct ttcaccagcc ggtgaaatcc gccgcccgcg aaatcgacca
     2341 gatagacttc gccggaggga tccaacgcga actcgaccgg ccgcacttgc gtgtcggcga
     2401 gctgccggtg ctcgaccacc ttgcccgtgc cgccgcgatc gccctcgtaa cgcagcagcc
     2461 acagccgccc ggtgtcgtag tcgccgtaaa ggaacgcccc cttcagctcc ggcagccgat
     2521 cgctgccgta aacgtagccg gccgtgatcg agcggaagtc gctgtgcggc tgctcgacga
     2581 cgggcagctc gaatgcggcc ggaccctgcg ggcgagtggg ccggaacggg tggttgccct
     2641 cgcggacgct ccaaccgtaa ttgccgccgc ggcggatgag atagaccatt tcccacaggt
     2701 cctgcccgac ttcggcggcc cacagccgcc tggtcggcgg gtcgaagctg aacttccaga
     2761 tctggcgatg gccgaacgac cagatttccg ggcgggcttt ctcgcggccg gcgaatggat
     2821 tgtccgcggg aatcgaatag ttcttgccgt ccgcgggccg atcgacgtcg atccgcagga
     2881 tcgccccgag caggtcatcg atcgtctggc cggtgagcag ctcgtcggcg atgccgctgc
     2941 cgtcgcccgt agccagatag aggtaaccat cggggccgaa gcggatgcaa ccgccgttgt
     3001 ggcctccggc gggccactcc aggatcacgg tctcgctggc agggtccgcc gccagcggct
     3061 gacccggatt gaccttgtag cgcgagagcc ggctgccgcg atcgggtgcg gggggcggat
     3121 cgatcagcga ggtgacgtag aagtagccgt tctcggcgaa cttcggatgg aaggccaggc
     3181 cgtaggtcgt tcgcttgacg tcgatcaaga ggtccgcttt atcgacgtcc ggccggattt
     3241 cgaacgagta gattttgccc ggccgagtgg cgacgccgaa gcggttttgc cccggcacga
     3301 cgccgaccga gagcggctcg aacagctcca gcttgggaaa cacgcgctcc gtccggtacg
     3361 ggtccggcgg ctccggcgtc ccgtggagat tgccggtggt ccactgctcg cggcgctcga
     3421 tgccataggg cttgcggacg ggctcagccg ccgaggcggc agccgtgatg aggaacagaa
     3481 tggtggcgcg cctaaacatg agcgaaat
//
LOCUS       DASRJB010000846         9097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1103844, whole genome shotgun sequence.
ACCESSION   DASRJB010000846 DASRJB010000000
VERSION     DASRJB010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103844"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFB80_22220"
     CDS             complement(<1..697)
                     /locus_tag="VFB80_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36569.1"
                     /translation="MSATLASANLAPPLRPLVETAAMFRQECTALDHLIEELYRDVEQ
                     LREELVRKSDEIEEGRRKLADRGRQLAEQRKEGGRLMALLEQQESRAGETLTEVKSLR
                     EQLAREREESAQREATRTAFLEQKMRAAETERDQLRHELNVLQATAAASGGAGGGSVE
                     SLAPLLSELGEMRRQVSETQAQLGQTQTQLGEARTQLGEAIERSVAAAAAQSPLVQAG
                     GGAPDRERLELESE"
     gene            983..1348
                     /locus_tag="VFB80_22225"
     CDS             983..1348
                     /locus_tag="VFB80_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36570.1"
                     /translation="MPSRRQSEQSAQLAEAGESRGAVALTVAWMLTCTSTAVAMLVVV
                     ALWLISLAFPFPGPRPHPLLVVAGLLQFVALFTGLLCLLFTPLAYRVRRTSPPRPITI
                     AAVLIGLAPIVTLLVRAFQ"
     gene            1345..2778
                     /locus_tag="VFB80_22230"
     CDS             1345..2778
                     /locus_tag="VFB80_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HZN36571.1"
                     /translation="MTDYLRETHHEFEAASQAALADPNLQRILGQLNDTLGRRNRDAW
                     AALPDSDLIRERARSIKDQTLAELDRHLETLERSVTSRGGRVHWAADGQEACRIIVEL
                     IRQRQATKVVKSKSMTSEEIHLNHALEEAGIEAVETDLGEYIIQLAGHRPSHIVAPAI
                     HLSAGEIADILSKPAGHRLPAQRVALAGFARQALRQKFAEAEIGISGANFAIAETGSI
                     VLVTNEGNGRLTCALPPCHIAVMGMEKVIPRLADLPCFLKVLARGATGQKLSIYTQVI
                     TGPRQPGELDGPEEFHLVILDNGRSRILGGPLRESLFCIRCGACLNACPIYRNVGGHA
                     YGGVYAGPIGAVLTPLYDGLAANHHLPHASSLCGACQAACPVKIQIPDLLIKLREQLH
                     EEPEQRSRLEAIAYGLWARILRNPWLYRVGSWLAARTIGRLKRGSPWIKKLPAKLAGW
                     TKTRDFPAPAAERFRDWWNREGRDEQS"
     gene            2765..3439
                     /locus_tag="VFB80_22235"
     CDS             2765..3439
                     /locus_tag="VFB80_22235"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HZN36572.1"
                     /translation="MSNRNAFLARVRQASEQGRQYRVHLQPVPPEIGYLGAEGDLCER
                     LAVEIDAVGGEAFVVPDLQAAGRQLHALLAEAGARSAVCWKHELLDRLGVADLLATAG
                     VSLFDYDDLVALSAAERRSKLLACDVGITSCDLAIAETGTLLMCSRPGQERLASLIAP
                     MHIAIVERSQIVPDLIDAFEWLPAESLPPNVTLITGPSKTGDIELQLTTGVHGPGKWR
                     VIVVNG"
     gene            complement(3436..3780)
                     /locus_tag="VFB80_22240"
     CDS             complement(3436..3780)
                     /locus_tag="VFB80_22240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36573.1"
                     /translation="MIDSLLMIGAILTVVVGSGALLGSAWAQSKENNTFSWTIVIRST
                     IVGVLLAGVAFGLGVWAWIMHHRWAEVRHTLFVIEALSSLALGSLIGWIAAFLLGRHW
                     HTPQPRSNRIPR"
     gene            complement(3777..4529)
                     /locus_tag="VFB80_22245"
     CDS             complement(3777..4529)
                     /locus_tag="VFB80_22245"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZN36574.1"
                     /translation="MNHDLRYALVMLAAIVVLSLLLRRWQARLPLAWWEKLGIGVGGF
                     CGAMIGAKLPFAVADWSGLLSGTTWLADGKTILCGIVGGYFGVELAKWTLDVRIKTGD
                     TFAVPVAVAVAIGRLACFVGGCCYGAPTSLPWGMTFARAGDSLPRHPTQLYEAAFHLT
                     LAAILLALQRRGGFRGQLIKLYILAYLAYRFLTEFIRPEPALWLGLTGYQWAALALSP
                     VFACLWWRDAVRPPDDKAAPVIANSKARGDPP"
     gene            complement(4526..5998)
                     /locus_tag="VFB80_22250"
     CDS             complement(4526..5998)
                     /locus_tag="VFB80_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN36575.1"
                     /translation="MKQASAHRDYAFLGTTQSLCPQCLRVVPAKIVERRGRVYFRKTC
                     AEHGTREDFVCSDVKWYDRMEFALPARMPAVFGTQAEKGCPLDCGLCSEHEQHTCVGV
                     LEITSSCNLTCPMCYAASSPGGKHLPFADCRRQIDRLVEVEGRAEICQLSGGEPTIHP
                     QFAEVVEYALSREIDYVMVNTNGIRFARDPALVELAARHRERLEIYFQFDGLSNGVYR
                     ALRGEELLETKLAALEALGRAGVHVTLVCTLQGGVNGDQLGPLVEFGLARPWITGISF
                     QPATYSGRHVLPETLEQRITFPDVIKGVAAQTAGVFAEEDFLPLPCAHPNCHQIAIAY
                     RQNGVATPVTRFIDAAENFDLLANGISFTRAKARDLIRQYLARQACCGGGGCATVQGS
                     KFDVQRSLPTLNFETETLNSPSDDFFSRILAEKLGSENLFRITITNFLDAYNFDVRRV
                     MKCCTHHVLPSGHVIPFCAYNVLYRDGHVPLPLLSERVMT"
     gene            complement(5995..6642)
                     /locus_tag="VFB80_22255"
     CDS             complement(5995..6642)
                     /locus_tag="VFB80_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36576.1"
                     /translation="MATAASLAHGDRLWYIVGRWEELGGEARVNLLRIIAVGAFYCVQ
                     LAQHFLLSQPTADSTRFHQQATAIAVAWTLVALAVLLSLRLRIFPAALKYISAACDLV
                     LLTVLASLAAGPSSPLVLAYFLVIALAALRFSLGLVWFSTLGAMAGYWLLVGLADAKT
                     SRWFDEQHAVPPATQLITLLSLGLTGVIIGQVVRRVKSLAEDYAQRRSAAAETPR"
     gene            complement(6722..7927)
                     /locus_tag="VFB80_22260"
     CDS             complement(6722..7927)
                     /locus_tag="VFB80_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HZN36577.1"
                     /translation="MITTASLKTQSWKDLAEMAKSKGLPGYSSMRKDQLVKALVRAAK
                     RRAQARAAAKSSKPAKGARRSTTLKAHAAHTNGSKSANGKLNGASAHQGRAERLTPRQ
                     QRIAERIQRANAERERLKDLSGAFTLAKNKRKAPDKDRLVLMVRDPYWLQACWTVCRL
                     SVKRAEAALAEHWHTAHPVLRLLEVDGGQTTSTSERIVREIDVHGGVTNWYIEVSNPP
                     QGFRVELGYKSAVGKFFSICRSNTVRTPAPDSSDVIDGNWTDVAENYERVFALSGGYT
                     EDNNSGELQELFEERLRRPMTPATTSQFGSGAERLLNRHRDLDFHVDAEMILYGRTKN
                     DAKVMLAGEPIKLRPDGSFTVRLAMPDKRQVLPVVASTPDGVEQRTIVIAVERNTKVL
                     EPMVREAND"
     gene            8332..8625
                     /locus_tag="VFB80_22265"
     CDS             8332..8625
                     /locus_tag="VFB80_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36578.1"
                     /translation="MDGIYGEYSARAATPMYASGPSMQVETAAIAPHKLARESSPPVG
                     AKHYPQSAYVEALDFVKKYTNYALGTLTTVFHLTTLPLRKHFTHSEVPAANGS"
     gene            8756..8995
                     /locus_tag="VFB80_22270"
     CDS             8756..8995
                     /locus_tag="VFB80_22270"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZN36579.1"
                     /translation="MAQAMEWVAKITTVAMEMFLPAVGGAYLDRRWGTNHWALIGVVL
                     GFVVGMWHLLQMTRTANKRRGKSSGEESSGESHDP"
BASE COUNT         1605 a         2978 c         2913 g         1601 t
ORIGIN      
        1 gctccgattc cagctccagt cgctcgcgat ccggagcacc accgccagcc tggacgagcg
       61 gcgattgagc agcggccgcc gcgaccgagc gttcgatcgc ctcgcccagc tgcgtgcggg
      121 cctcgccaag ttgcgtttgg gtctggccga gctgagcctg cgtctcggag acctgccggc
      181 gcatctcgcc caattcgctg agcaagggtg ccagcgactc gaccgacccg cctccggctc
      241 cgccgctggc ggcggccgtg gcttgcaaga cgttcagctc gtgccgcaac tggtcgcgtt
      301 cggtttccgc cgctcgcatc ttctgttcca ggaatgctgt gcgggtcgct tcgcgctgcg
      361 ccgattcttc ccgctcgcgc gccagctgct cgcggagcga cttgacttcc gtcagcgttt
      421 cacccgcccg ggattcctgc tgctccagca aggccatgag ccggccgccc tccttgcgtt
      481 gctcggccag ctgccggccg cgatccgcca gcttgcggcg gccctcctcg atctcgtcgc
      541 ttttgcgaac cagctcttcg cggagctgct ccacgtcgcg gtacagttct tcaatcaggt
      601 ggtcgagggc ggtacattcc tgccgaaaca tggcggccgt ttccaccaaa gggcggagcg
      661 gcggagccag gttggcggag gcaagtgttg cagacacggc ccgttccttt cgcagcagcg
      721 cggggggacg ccgatcgtgt ggtgtgtcga gcgcgcgaca ggccagggct ggaggagtcc
      781 tcccgtcggt acatccgcgc tcttggcggc cgccagagga gcaggacgat ccgccagcga
      841 aaccttacgt tacgaacttg cgggcggcaa acggctggca ggccgccccg cggaatggac
      901 caggggcgat atgatgaagc cgccggcaac ggagtttctg aatgctccgc gttgccaaaa
      961 gtcatcgccc gcgggcctcc gaatgccctc tcgccgccaa tccgagcaat ccgcacaact
     1021 ggccgaagcc ggcgaatcgc gcggggccgt cgccctgact gtggcctgga tgctcacctg
     1081 caccagcacg gctgtggcga tgctggtggt ggtggcgctc tggctgataa gtctggcctt
     1141 tccgtttcca ggaccgcggc cgcatccgct cctggtggtc gcgggcctgc tccagttcgt
     1201 ggcgctgttc acgggcctgc tctgcctcct gtttacgcct ctggcctatc gcgtccgccg
     1261 gacctcgccg ccgcggccga ttacgatcgc tgccgtactg attggtcttg cgcccatcgt
     1321 cacgctactg gtccgcgcct tccaatgacc gactacctgc gcgaaacgca ccatgagttc
     1381 gaggcggcgt cgcaggcggc gctggccgat ccgaatttgc agcggatcct ggggcagctc
     1441 aacgacacgc tcggccgccg caaccgcgac gcctgggccg cgctgcccga ttccgacctg
     1501 atccgcgagc gggcccgctc gatcaaggac cagacgctgg ccgagttgga ccggcacctg
     1561 gaaacgctgg agcgaagcgt caccagccgc ggaggacgcg tgcactgggc cgcggacggc
     1621 caggaggcct gccgcatcat cgtcgagttg atccgccagc ggcaggcgac caaggtcgtc
     1681 aagtccaagt cgatgaccag cgaggaaatt cacctcaacc acgcgctgga ggaagcgggc
     1741 atcgaggcgg tggaaaccga tctgggcgaa tacatcatcc agctcgccgg ccaccggccg
     1801 tcgcacatcg tcgcaccggc gatccacctc tccgccggcg agatcgccga catcctcagc
     1861 aagcctgccg gtcacaggct gcccgcccag cgcgtcgcgc tcgccggttt tgcccgccag
     1921 gctctgcggc agaagttcgc cgaggccgag atcggcattt cgggagccaa ctttgcgatc
     1981 gccgagaccg gctcgatcgt gctggtcacc aacgaaggca acggccggct aacctgtgcc
     2041 ctgcccccct gccacatcgc cgtcatggga atggagaagg tcattccgcg gctggccgac
     2101 ctgccgtgct ttctcaaggt gctggcccgc ggtgcgaccg gccagaagct gtcgatctat
     2161 acgcaagtca tcactggccc gcggcagccg ggcgagctgg acggcccgga agaattccac
     2221 ctggtgattc tcgataacgg gcgctcgcgg attctcggcg gcccgctgcg ggaaagcctg
     2281 ttctgcatcc gctgcggagc ctgcctcaac gcctgtccga tctatcgcaa cgtcggcggc
     2341 catgcctacg gcggcgtcta tgccgggccg atcggcgcgg tgctgactcc gctctatgac
     2401 gggctggccg ccaatcatca tctgccgcat gcgtcgagct tgtgcggcgc gtgccaggcg
     2461 gcgtgtccgg tgaagattca gatccccgat ctgttgatca agctgcgcga gcagctgcac
     2521 gaggagcccg agcagcgcag ccggctggag gcaatagctt acgggctctg ggcgcggatc
     2581 cttcgcaacc cttggctgta tcgcgtcggt agttggctcg cggcacgcac gatcggccgc
     2641 ctcaagcgcg gttcgccctg gatcaagaag ctgccggcga agcttgccgg ctggaccaag
     2701 acgcgcgact ttcccgcgcc ggcggcggaa cgcttccgcg actggtggaa tcgagagggc
     2761 cgcgatgagc aatcgtaatg cctttctggc gcgcgtccgg caagcgtccg agcaagggcg
     2821 gcaataccgg gtccatttac agccggtgcc gcccgagatc ggctatctcg gcgcggaggg
     2881 cgacctgtgt gagcgattgg cggtcgaaat cgacgccgta ggcggcgagg cgtttgtcgt
     2941 ccccgacttg caagcggctg gccgacagtt gcacgcgctc ttggcggaag ccggcgctcg
     3001 ctcggccgtc tgctggaagc acgagttgct ggaccggctg ggagttgctg acctgctcgc
     3061 gactgcaggt gtcagtttgt tcgactatga cgacctggtc gcgctctctg cggcagagcg
     3121 acggagcaaa ctgctggcct gcgacgtcgg cattaccagc tgcgacctgg ccatcgcgga
     3181 gacgggcacg ctgctcatgt gttcgcgccc agggcaagag cgtctcgcct cgctgatcgc
     3241 gccgatgcac atcgccatcg tcgagcgaag tcagatcgtg cccgacctga tcgacgcctt
     3301 cgagtggctg cccgccgaat cgctgccgcc caacgtgacg ctgatcaccg gtccgagcaa
     3361 gaccggcgac atcgagctgc aactgacgac gggcgtccat gggccgggca agtggcgcgt
     3421 gatcgtcgtc aatggttagc gcggaattcg attgctcctc ggctggggag tatgccagtg
     3481 cctgccgaga aggaaagcgg caatccagcc gatcaggctg ccgagtgcca ggctggagag
     3541 ggcctcgatg acgaacagcg tatgtcgcac ctcggcccag cgatggtgca tgatccaagc
     3601 ccacacaccc aacccgaagg ccacgccagc cagcagcacg ccgacgatcg tgctgcggat
     3661 cacaatggtc caggagaacg tgttgttctc tttcgattga gcccaggcgc tgcctaacag
     3721 agcgccgctg cccacaacga cggtcaaaat tgccccgatc atcaacagcg agtcgatcat
     3781 ggtgggtctc cacgtgcctt tgagttggcg atgaccggtg ctgccttgtc gtccggcggc
     3841 cgcacggcgt ctcgccacca taggcacgca aagaccggag atagggcgag ggccgcccat
     3901 tgatagcccg tcaaacccag ccacagggcg ggctccgggc ggatgaactc cgtgaggaag
     3961 cggtaagcca gatacgccag gatgtagagt ttgatcaatt ggccgcggaa gccgccccgc
     4021 cgctgaagag cgagcagaat ggcggccagc gtcagatgga atgcggcttc gtagagctgc
     4081 gttggatgcc tgggcaacga atcgccggcg cgggcgaagg tcatgcccca aggcaggctg
     4141 gtcggcgcgc catagcagca gccgccgaca aagcaggcga ggcgtccgat cgccacggcc
     4201 accgcgaccg ggaccgcgaa cgtgtcgccg gttttgatgc ggacgtcgag cgtccacttg
     4261 gccagctcga cgccgaaata cccgccgacg atgccgcaca ggatcgtctt gccatcagcc
     4321 agccaggtag tgccactaag caatccgctc cagtcggcta ccgcgaacgg cagcttggcc
     4381 ccgatcatcg ccccgcaaaa gccgccgaca ccaatgccca gcttctccca ccacgccagc
     4441 ggcagacggg cctgccaccg ccgcaagagc aggctgagca cgacaatggc cgcgagcatg
     4501 accagggcat agcgcaggtc gtggttcacg tcatcacccg ctcggacaag agcggcagcg
     4561 gcacgtgtcc gtcgcggtag aggacgttgt aggcgcagaa cggaatcacg tggccgctgg
     4621 gcagcacgtg gtgcgtgcag cacttcatca cgcggcgaac gtcgaagttg taggcgtcga
     4681 ggaagttcgt gatcgtgatg cggaagagat tctcggagcc gagtttctcg gcaaggattc
     4741 gcgagaagaa gtcgtcgctg ggggagttca aagtttcagt ttcaaagttc aaagttggca
     4801 gcgaacgttg aacatcgaac tttgaacctt gaactgtggc gcagccgcca ccgccgcagc
     4861 acgcctgccg cgccagatac tggcgtatca aatcgcgcgc cttggcccgt gtgaagctga
     4921 tgccgttggc caagaggtcg aagttctcgg cggcgtcgat gaatcgggtc acgggcgtgg
     4981 cgacgccatt ctgtcgatag gcgatcgcga tctggtggca attcggatgc gcacagggca
     5041 gcggcagaaa gtcctcctcg gcaaagactc cagcggtctg ggctgcgacg cccttgatca
     5101 cgtcgggaaa cgtgattcgc tgctcgagcg tttcgggcaa aacatgccgc cccgaatagg
     5161 tagcgggctg aaagctgatg ccggtgatcc agggccgtgc caggccgaac tcgacgagcg
     5221 gccccagctg gtcgccgttg acgccgccct gaagcgtgca gacgagcgtt acgtgtaccc
     5281 cagcccgccc gagggcctcc aatgccgcca gcttggtctc gagcagctcc tcgccgcgta
     5341 gcgcgcgata gacgccgttc gagaggccgt cgaattgaaa gtagatctcc agccgctcgc
     5401 gatggcgggc cgccagctcc accagtgccg gatcgcgggc aaagcgaatc ccgttggtgt
     5461 tgaccatcac atagtcgatt tcccgcgaca gcgcgtattc gacgacctcc gcgaactgcg
     5521 gatgaatcgt gggctcgccg cccgagagct ggcagatttc ggctcgcccc tcgacctcga
     5581 ccaacctgtc gatctgccgg cggcaatcgg cgaagggcaa atgcttgccg ccaggactgc
     5641 tggcggcgta acacatcggg caggtcaggt tgcaggagct ggtgatctcc agcaccccga
     5701 cgcaggtgtg ctgctcgtgc tcgctgcaca ggccgcagtc gagcgggcaa cccttctccg
     5761 cttgcgttcc aaacaccgcc ggcatccgcg ccggcagcgc gaactccatc cggtcgtacc
     5821 atttgacgtc cgagcaaacg aagtcttccc gcgtgccgtg ctccgcgcac gttttgcgga
     5881 aatagacccg cccgcggcgc tcgacgatct tggccggcac tacgcgcagg cactgggggc
     5941 agaggctctg cgtcgtgccg aggaatgcgt aatcgcgatg cgctgaggcc tgcttcatcg
     6001 cggagtttcc gccgcggcgc tgcgccgctg ggcatagtct tcggcgagcg atttcacccg
     6061 ccgcacgacc tgaccaatga tgactccggt caatcccagg ctaagcaacg taatcaactg
     6121 cgtcgccggc ggcacggcat gctgctcgtc aaaccagcgg ctggtcttgg cgtcggccag
     6181 cccaaccagc agccaatagc cggccattgc gccgagcgtg ctgaaccaga ccaggcccag
     6241 gctgaagcga agcgcggcca gcgcgataac gagaaagtac gccagtacga gtgggctaga
     6301 ggggcccgcg gcgagcgacg ccaagaccgt aagcaggacc aagtcgcacg ccgcagagat
     6361 gtatttcaga gctgcgggaa atatccgcaa gcgaagcgac aggagcactg ccagggcgac
     6421 gagcgtccag gccacggcga tggcggtcgc ctgctggtgg aaccgggtcg agtcggcggt
     6481 tggctgcgag agcagaaaat gctgggcgag ctggacgcag tagaacgcgc cgaccgcgat
     6541 gatccgcagc aaattgacgc gcgcttcgcc gcccagctct tcccagcggc cgacgatgta
     6601 ccacagtcgg tcgccgtgcg ctagactggc ggcggtcgcc atgagatcct ccgagttgaa
     6661 tcaacggagg cgattgtcgc tggcggcgcg gcccggagca agcgaggacg ctcgccctac
     6721 gctagtcgtt ggcctcgcgg accatcggct cgagcacctt ggtgttccgc tcaacggcga
     6781 tcacgatggt gcgctgttcg acgccgtcgg gagtgctggc gacgacgggc aagacttgcc
     6841 gtttgtcggg catcgccagg cggacggtga acgagccgtc ggggcgaagc ttgatcggct
     6901 cgccggccag catcaccttc gcgtcgttct tcgtgcggcc gtagaggatc atctcggcat
     6961 caacgtgaaa gtcgaggtcg cgatgccggt tcagcaggcg ctcggccccg ctgccgaatt
     7021 gcgaggtggt ggccggcgtc atcgggcggc ggaggcgttc ttcaaacagc tcctgcaact
     7081 cgccgctatt gttgtcttcc gtgtaaccgc cgctgagcgc gaacacgcgc tcgtaattct
     7141 cggccacgtc ggtccagttg ccgtcgatca cgtcactgct gtcgggcgcg ggcgtgcgta
     7201 cggtgttgct gcggcagatc gagaagaact tgcccaccgc cgatttatag ccgagctcga
     7261 cgcgaaaccc ttgcggcggg ttggacacct cgatgtacca gttcgtcacg ccgccgtgga
     7321 cgtcaatttc gcgaacgatc cgctcggagg tgctcgtcgt ctggccgcca tcgacctcga
     7381 gcagccgcag caccggatgc gccgtgtgcc aatgctcggc caacgcggct tcggcccgct
     7441 tcacgctcag gcggcaaacg gtccagcacg cctgcagcca gtaaggatcg cggaccatca
     7501 gcacgaggcg gtccttgtcc ggcgccttgc gcttgttctt ggccagcgtg aacgctcccg
     7561 acagatcctt cagccgttcg cgctcggcgt tggcccgctg aatgcgctcg gcgatccgct
     7621 gctgccgggg agtaagccgc tcggccctcc cctggtgcgc agacgcgccg ttcagcttgc
     7681 cattggccga cttcgaaccg ttggtgtggg cggcatgcgc cttcagcgtc gtgctgcgcc
     7741 gcgcgccctt cgccggcttg gaggactttg cagccgcccg cgcttgagct cgcctcttgg
     7801 cggcccgaac cagagctttg accagctggt ccttgcgcat cgagctatag cctggcagtc
     7861 ccttgctctt ggccatttcg gccaagtcct tccagctctg tgttttgaga gacgctgtgg
     7921 taatcaaggg acggtgacct ccgctgtgcc tacgacgcgt gccgcatgat ggatacggct
     7981 cgcgcggaac gacggtttcc aagtcgtccg tgacgttcca gcctagcgga aatccctctc
     8041 cgtcctgcgg gcactctgct gttgtggatc atcgctgggc gattacccac aagtcatctt
     8101 ggattcgagt ctggcgactg gaatccgcct cccactgctc accattcgca attacacccc
     8161 gccgctgtct ctaagaccaa tcggcgaggg aacttacgat ccggcgatcc agcgtctgcg
     8221 cggccactct agttgttctg ccgggcaggc atcgaagagg tcgaggaacc catcgacacc
     8281 tcccatcaat ctaatcaagc ggttgccgga acgcaacacg ccccctgacc agtggatggc
     8341 atttatggcg aatattccgc ccgtgccgca acaccaatgt acgcgagcgg cccatccatg
     8401 caggtcgaaa ccgccgccat cgcaccacac aagttagccc gagagtcgtc gccgccagtt
     8461 ggcgcaaaac attatcccca aagcgcttat gtcgaagccc tggactttgt gaaaaaatac
     8521 acgaattatg ccctgggtac gttgaccact gtttttcacc tgactacatt accgcttcgc
     8581 aagcatttta cgcacagtga agtgccagcc gcgaacggct catgagccag attgagcgat
     8641 ttcgcctcgc cagcaacgca gcgcaaagct ttctcacgaa atgatttgcg actctttgtg
     8701 gcgggacgag ttgctcccca aggccgtgac tgacaaacgc cccagcccgc ttccgatggc
     8761 gcaggccatg gagtgggtgg ccaagatcac gaccgttgca atggagatgt tcctgccggc
     8821 cgtcggtgga gcgtatctcg accgccgctg gggcacgaac cattgggcgc taattggtgt
     8881 ggtgttgggt ttcgtcgtcg gcatgtggca tttgctgcaa atgacgcgaa ccgcgaacaa
     8941 gcggcgcggc aagtcgagcg gcgaggaatc gtccggtgaa tcgcacgacc cataggcgac
     9001 tggaaccgcg ctcatcactg atgagcctca tcgcttcttg cgcgctaatg gctgtcgtca
     9061 tcgcgcccgc ttcactgctg atcgccgccc tcgccag
//
LOCUS       DASRJB010000847         7160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3628160, whole genome shotgun sequence.
ACCESSION   DASRJB010000847 DASRJB010000000
VERSION     DASRJB010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3628160"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..666)
                     /locus_tag="VFB80_22275"
     CDS             complement(52..666)
                     /locus_tag="VFB80_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HZN36580.1"
                     /translation="MTTVLITAFEPYDDWPENSSWLALMELTRDLPAQPQVVTRRYPV
                     DFEAARARLTEDLAANYDFALHLGQAPGISRVHLEAIGINIGGHSGQSPDQFQPLAPG
                     GPAAYQSALPLADWSAKIRELGIPCQVSYHAGTYLCNALLYLTHHIAAGGRLKTRAAF
                     IHFPLAPAQVLDPRQGGPSLPSSQCAAAVRLILHELPSLGERWT"
     gene            749..1372
                     /locus_tag="VFB80_22280"
     CDS             749..1372
                     /locus_tag="VFB80_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN36581.1"
                     /translation="MNRRHITLAALLLGCLASQALAGKYNKTLSIGDSGPDFSNMEGT
                     DGKTHSLADLKDKDVVVLCFTCNTCPYAVDYEDRLIALAKKFESEGGKAAIVAVNPNL
                     VTDDLLPAMQERAKTKGFNFLYIHDASRQQVAKSYGATYTPEFVVLNKARKVVYLGAM
                     DDSPDGKKITKKYVEDAVAAVLAGKSPEITETPAVGCAVRYAKERGK"
     gene            complement(1432..2280)
                     /gene="aroE"
                     /locus_tag="VFB80_22285"
     CDS             complement(1432..2280)
                     /gene="aroE"
                     /locus_tag="VFB80_22285"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN36582.1"
                     /translation="MSSAPLQEVVCCLGQPVAGNPTQYMMEKAFAAAGLDWKYLTCEV
                     PPEKLADAVKGLRALGFKGANFTIPHKVAVVPLLDGLSPAAELMGAVNCANRVSEKLV
                     GENTDGKGFVQSLRQFGDLAGRNVVLLGAGGAARSIAVELALGGVQRITVVNRDAARG
                     QALVDLLRQRTKTAAELTPLNGAYPVPPDAHVFINATSVGLGDADARVPLAADSLRPE
                     LIVADVIFNPPRTWLIRTAADRGCRTLDGLGMLVNQAVISFRIWTGREPDAGVMRDAL
                     EEYLEI"
     gene            complement(2290..3000)
                     /locus_tag="VFB80_22290"
     CDS             complement(2290..3000)
                     /locus_tag="VFB80_22290"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZN36583.1"
                     /translation="MPELGVNIDHVATLRQARKTYEPDPVAAAVLAELGGADAITIHL
                     REDRRHIQDRDVRLLKETVPIKLNLELSVADEIVGIACQVKPDQATIVPERREEVTTE
                     GGLDVVTHKAAVAKAAQRLRAAGIKVSLFLDPDPRQIELAGQLGADAVELHTGQYALT
                     RGAARHQQLVALTNAGKLVKDLGLALHAGHGLNYHNVIPVARIPQMHELNIGHAIVSR
                     AVFVGLERAVREMKHLVG"
     gene            complement(3081..4556)
                     /locus_tag="VFB80_22295"
     CDS             complement(3081..4556)
                     /locus_tag="VFB80_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36584.1"
                     /translation="MFANPDVTLARRSFLRGSAGGFGIAALASLLNPQLLADDKVSRQ
                     IPRGAALPGVLAQPHFPAKAKRVIYLCMSGGPSQIDLFDYKPKLKDLNGTELPGSVRM
                     GQRITGMTSGQKSFPCAAPQFKFEQHGQCGAWISELLPHTAGIVDDIAIVKTLNTEAI
                     NHDPAFTFLQTGSQQPGRPSFGAWLSYGLGSENDNLPAFIVMISQGSGNKTDQPIFSR
                     LWGSGFLPSQHQGVRFRAGADPVLYLSNPAGIDGESRRSMLDGVAELNKLAAHSIGDP
                     EISTRIAQYEMAFRMQTSLPELTDLSGESKETLDLYGIKEDGVDGGFARNCLLARRMA
                     ERGVRFIQLMHRGWDQHSSLPKQIRGQCQDVDRPSAALVKDLKDRGLLDETLVIWGGE
                     FGRTVYSQGSLTPTNYGRDHHGRCFTLWMAGGGIKPGLSWGQTDDYCYNIVEGGVHVH
                     DFQATALHCLGIDHTRLTFRFQGRDFRLTDVHGKVVKELLA"
     gene            complement(4558..5313)
                     /locus_tag="VFB80_22300"
     CDS             complement(4558..5313)
                     /locus_tag="VFB80_22300"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN36585.1"
                     /translation="MAVTLKKSRWAVPAGNPLLHSGDHLTQREFHRRYEAFPDSEARF
                     ELIGGVVYMMSPAGYDHGRGDYRISGLLFQYERATPGVAGAQNVTIILGAASEPQPDN
                     VLLVRPEYGGRVQIKRQGKTRYIVGPPELVFEVAHSSLSIDLHEKRRDYQTSGVLEYI
                     VLDLEDNEVHWFDLATDEPLVLPADRILRSHAFPGFWLDTRALVTGDVARLAACLDQG
                     LSSPEHQRFVVQLAAHRKQRPASRGKRGRQNGA"
     gene            complement(5322..>7160)
                     /locus_tag="VFB80_22305"
     CDS             complement(5322..>7160)
                     /locus_tag="VFB80_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN36586.1"
                     /translation="RFADTHGYHIDSGRDMSRWREYVIESFNRNVPYDQFTVEQIAGD
                     LLPETGDREQDLRQKLASGFNRNHMINFEGGAIPQEYLNAYLVDRVNTTATVFLGLTV
                     ACTQCHDHKYDPLTQREFYQLYAFFNNLPENGLDGRKGNAVPLLSFPTPQQQAELDSL
                     AAEIKAAEARVAAAESSQDPPPDAEAVKKLKADVAALRKRQTDAQAAVANSMVMQEMP
                     QPRDTFILVRGAYDKQGEKVTADVPAFLPPLPKADQQNRLSLARWLVSAEQPLTARVT
                     VNRYWQLLFGTGLVRTAEDFGAQGELPSHPGLLDWLAVEFRESGSKFKVQSSKLEDVV
                     GPAKAVLSTQYSVPGTEYAADSGWDVKQLVRSIVSSSTYRQSSQITPALRAKDPENRL
                     LARGPRFRLQAEFIRDQALFHSGLLDGHVGGKSVSPYQPAGLWEELMSRADGDNWTAQ
                     KYKQDHGVDLYRRTMYTFWKRTCPPPTLATLDAPDRETCTVRRSRTNTPLQALVLLND
                     PTYVEAARKFAERILRDGGQSLDERLTFAFRSVLARQPTTAERAVLRSVYERQLARFR
                     ELPDAAKALLAVGESPADATLDPAELAAWTMVASAILNTDEAVTKG"
BASE COUNT         1243 a         2287 c         2345 g         1285 t
ORIGIN      
        1 atagcggagc gaactcgtgc cgcatccagt cgaacacgca atcgctgagc gtcatgtcca
       61 tcgctcgccg agagagggca actcatgcag gatcagccgc accgccgcag cgcactggct
      121 gctcggcaga ctcggcccac cctgccgcgg gtcgagaacc tgcgcaggag ctaacgggaa
      181 gtggatgaac gcggcccgcg tcttcagccg tcccccggct gcgatatggt gagtcaggta
      241 gagcaaggcg ttacagagat aagtgcccgc gtggtacgag acctggcacg gaatgccgag
      301 ttcacggatt ttcgccgacc agtcggccag gggcagcgcg gactgataag ccgccggtcc
      361 gccaggcgcc agcggctgga attgatcggg cgattgcccc gagtggccgc cgatgttgat
      421 gccgattgcc tccaggtgca cgcggctgat gcccggcgcc tgccccaggt gcaaggcgaa
      481 atcgtaattc gccgccaggt cctccgtcag ccgggcgcgg gcggcttcga aatcgacggg
      541 atagcggcgc gtcacgacct gcggctgcgc gggcaggtcg cgggtcaact ccatgagggc
      601 cagccagctg gagttttccg gccagtcgtc gtagggctcg aacgcggtga tcaacacggt
      661 ggtcatcgaa actccgccgt ccactatact ggtttcgtgg gtcaggtgac tggcccctca
      721 cggagcaagc gaagtcgaaa ggtccaacat gaatcgccga cacatcacgc tggccgcctt
      781 gctgctcggc tgtctcgcct cgcaggcgct ggcaggcaaa tacaacaaga cgctcagcat
      841 cggcgacagc ggcccggact ttagcaacat ggagggcacg gacgggaaga cgcattcgct
      901 agcggacctc aaggacaagg acgtcgtcgt cctttgcttc acctgcaaca cctgtcccta
      961 tgccgtggac tacgaggatc gcctgatcgc cctggcgaag aaattcgagt cggaaggggg
     1021 caaggcggcg atcgtcgccg tcaatccgaa tctggtgacc gatgacttgc tcccggcaat
     1081 gcaggagcgg gcgaagacga agggattcaa ttttctctac attcacgacg ccagccggca
     1141 gcaagtcgcc aagagctacg gtgcgaccta cacgccggag ttcgttgtgc tgaacaaggc
     1201 gcgcaaagtc gtctacctgg gcgcgatgga cgacagccct gacgggaaga agatcacgaa
     1261 gaagtacgtc gaagatgccg ttgccgcggt tctcgccggc aagtcgcccg agatcaccga
     1321 gacgcccgcc gtcggctgcg cggtgcggta tgccaaggag cgcggcaaat agtatccgtc
     1381 acactccgcg tgacgagccg cctcacgcgg agcgtgagga cgactgtggg atcagatctc
     1441 caagtactcc tcgagcgcgt cgcgcatcac gccggcgtcc ggttcgcggc ccgtccagat
     1501 gcgaaagctg atgaccgcct ggttgacgag catgcccagg ccatcgagcg tgcggcagcc
     1561 gcggtcggcg gctgtgcgga tgagccaggt tctcggcggg ttgaaaatca cgtcggcgac
     1621 gatcaattcc ggccgcaggg aatcggcggc gaggggcaca cgggcatcgg cgtcgcccag
     1681 tccgacggaa gtggcgttga tgaaaacgtg cgcgtccggc gggaccggat aggcgccgtt
     1741 gagcggcgta agctctgccg cggtcttggt gcgctgccgc agtagatcaa ccagcgcttg
     1801 cccgcgggcg gcgtcgcgat tgacgaccgt gatgcgctgg acgccgccga gggccaattc
     1861 gaccgcaatg gaccgcgccg cgccgcccgc tccgagcagg acgacgttgc gtcccgcgag
     1921 gtcgccgaac tgccgcagcg actgcacgaa gcccttgccg tccgtgttct cgcccacgag
     1981 cttctcgcta acgcgattgg cgcagttgac cgcgcccatc agctccgcgg ccgggctgag
     2041 cccatcgagg agcggaacga cggcgacctt gtgcgggatc gtgaagttcg cgcctttgaa
     2101 acccagtgcc cgcaatccct tgacggcatc ggccagcttt tcaggcggga cttcgcaagt
     2161 gagatatttc caatccaagc ccgccgccgc gaaggctttt tccatcatgt actgcgtggg
     2221 attgccggcg acgggctggc cgaggcaaca gacgacttcc tgaagcggcg cggacgacat
     2281 ggcgtgatct tagccgacca ggtgcttcat ttcccgcacg gcccgctcga gcccgacgaa
     2341 caccgcgcgg gacacgatcg cgtggccgat gttcagctcg tgcatctgcg gaatgcgggc
     2401 gacgggtatg acattgtggt agttcagacc gtgccccgcg tgcaaggcca ggccgaggtc
     2461 cttaacgagc ttgcccgcgt tcgtcagggc gacgagctgc tggtgccgcg ccgcgccgcg
     2521 agtgagtgca tattggccgg tgtgaagttc aaccgcgtcc gcgccgagct gcccggccag
     2581 ctcgatctgc cgcggatcgg gatcgagaaa caggctgact ttaatgccgg ccgcccgcag
     2641 gcgctgggcc gccttcgcca cggccgcctt gtgcgtgacg acgtcgaggc ccccctcggt
     2701 cgtcacttct tcgcgccgct ccggcacgat cgtcgcctgg tcgggcttga cctggcaggc
     2761 gatccccacg atttcgtcgg cgaccgacag ctccagattc agcttgatcg gcaccgtttc
     2821 cttgagcagc cgcacgtcgc ggtcctggat gtgccggcgg tcctcgcgaa ggtggatggt
     2881 gatcgcgtcc gcgccgccca gttcggccag cacggctgcc gccaccgggt ccggctcata
     2941 cgtcttgcgg gcctggcgga gggtcgcgac gtggtcgatg ttgacgccga gttcgggcat
     3001 ttgtcgggag tcaggggtcg ggagtcagga gtcggcagga ccgctgcaag acatggacgg
     3061 cggcgaattg ttcgcgccaa tcaggccaac agctccttca ccaccttccc gtgcacgtcg
     3121 gtgaggcgga agtcgcggcc ctggaagcgg aaggtgaggc gggtgtggtc gatacccagg
     3181 caatgcagcg cggtggcctg gaagtcgtga acgtgcaccc cgccctcgac gatgttgtag
     3241 cagtagtcgt cggtctggcc ccaggagagg ccgggcttga tgccgccgcc ggccatccat
     3301 agcgtgaaac agcggccgtg atggtcgcga ccatagttcg ttggcgtgag cgagccctgg
     3361 ctgtaaaccg tgcggccgaa ctcgccgccc cagatgacca gcgtctcgtc caacaggccg
     3421 cggtccttca ggtctttcac cagtgccgcg ctcggccggt cgacgtcctg gcactggccg
     3481 cggatctgct tcggcaggct gctgtgctgg tcccagccgc ggtgcatgag ctggatgaac
     3541 cgcacgccgc gctcggccat tcgccgcgcc agcaggcagt tgcgggcgaa accgccatcg
     3601 acgccgtcct ccttgatgcc gtacagatcg agcgtttcct tggactcgcc cgacaggtcg
     3661 gtcagctcgg gcaggctggt ctgcatccgg aatgccatct cgtactgggc gattcgcgtg
     3721 ctgatctccg ggtcgccgat cgaatgcgcc gcgagcttgt tcagctccgc cacgccgtcg
     3781 agcatgctcc gccgggactc gccgtcgatg cccgccgggt tcgacagata cagcaccggg
     3841 tcggccccgg cccgaaaccg cacgccctga tgctgcgacg gcagaaagcc gctgccccac
     3901 aatcgcgaga agatcggctg atcggttttg ttgccgctgc cttgcgagat catcacgatg
     3961 aacgccggca ggttgtcgtt ctcgctcccc aggccgtagc tcagccaggc cccaaagctg
     4021 ggccgccccg gctgctgcga gccggtctgt aagaacgtga acgccggatc gtggttgatc
     4081 gcttccgtgt tcagcgtctt gacgatcgca atgtcatcga cgattcccgc cgtgtgcggc
     4141 agcagctcgc tgatccacgc gccgcactgg ccatgctgtt cgaacttgaa ctgcggcgcg
     4201 gcgcagggga acgacttctg gcccgacgtc atgccggtga tccgctgccc catccgcacc
     4261 gaaccgggca actccgtacc gttcaggtcc ttgagcttcg gcttgtagtc gaacaggtcg
     4321 atctggctcg ggccgcccga catgcaaagg tagatcaccc gcttggcctt cgccggaaaa
     4381 tgcggctgcg cgagcacgcc cggcagcgcc gccccgcggg gaatctggcg gcttaccttg
     4441 tcgtcggcga gcaactgcgg attgaggagc gacgcgagcg ccgcgatacc aaatccgcca
     4501 gctgagccac gaaggaaact tctgcgagcc agtgtcacgt ctgggtttgc aaacatttca
     4561 cgcaccattt tgccttcctc gtttaccacg agacgcaggg cgctgcttcc gatgagcggc
     4621 gagttgcacc acgaagcgct gatgctcagg gctgctcagc ccttgatcga ggcaagcggc
     4681 caggcgcgca acatcgcctg tgaccagcgc ccgcgtatcg agccagaatc cggggaaagc
     4741 gtgacttcgc aagatacgat ccgccgggag gacgagtggt tcatcggtcg cgagatcaaa
     4801 ccaatggact tcattatcct ccaggtccag cacgatgtac tccaggaccc cgctggtctg
     4861 gtagtcgcga cgcttctcgt gcaagtcgat cgacaggctg gaatgagcga cttcaaacac
     4921 cagttccggc ggtcccacga tgtaccgcgt cttgccttgc cgcttgatct ggactcggcc
     4981 tccatattcc ggccggacga gcagcacatt gtccggctgc ggctcgctcg cagcgcccag
     5041 gatgatcgtc acgttctgag cgcctgcgac gcccggcgtc gcccgctcgt attgaaacag
     5101 caacccggaa atgcggtagt cgccgcggcc atgatcgtag ccggctggag acatcatata
     5161 gacaactccg ccgatcagct cgaacctggc ttctgagtcg ggaaacgctt cgtagcgacg
     5221 atgaaattcg cgttgcgtca ggtggtcgcc cgaatgcagc agcggatttc ccgctggaac
     5281 ggcccatcgt gattttttga gcgtgacggc catggctgcc cctatccctt cgtgaccgct
     5341 tcgtccgtgt tcaggatcgc gctggcgacc atcgtccagg cggcgagctc ggcgggatca
     5401 agcgttgcat cggcgggcga ttcgccgacg gccaagaggg ctttggcggc gtcgggcagt
     5461 tcgcggaaac gggcaagttg ccgctcatag acgctacgca ggaccgcccg ctcggcggtg
     5521 gttggctgcc gggcgagcac gctgcggaaa gcaaacgtca gccgctcgtc gagcgactgg
     5581 ccgccatcgc gcaggattcg ctcggcgaac ttgcgggcgg cttcgacgta ggtcgggtcg
     5641 ttcaataaga ccagcgcctg tagcggtgtg ttggtgcggc tgcggcggac agtgcaggtc
     5701 tcgcggtcgg gggcatcgag cgtcgccagc gttggcggcg ggcaagtccg cttccagaag
     5761 gtgtacatcg tccggcgata cagatcgacg ccgtgatcct gcttgtattt ctgtgccgtc
     5821 cagttgtcgc cgtcggcccg cgacatcagc tcctcccaca atcccgccgg ctgatagggc
     5881 gagacgctct tgccgccgac atggccgtcg agcagcccgc tgtgaaacag agcctggtcg
     5941 cgaatgaact cggcctgcaa gcggaatcgc ggcccgcggg ccagcagccg attctccggg
     6001 tccttggccc gcagcgcggg cgtgatttgt gaggactggc gatacgtgga cgacgagacg
     6061 atagagcgga cgagttgctt tacgtcccat cccgaatctg ccgcgtactc agtaccgggt
     6121 actgagtact gagtactaag tacggccttt gccggtccaa cgacatcctc caactttgaa
     6181 ctttgaacct tgaactttga acccgattcg cgaaactcca ccgccagcca atcgagcagc
     6241 cccggatgac tcggcagctc gccttgtgcg ccaaagtcct ccgcggttcg caccaggccc
     6301 gtaccaaaca gcagctgcca gtagcgattg accgtcacgc gggccgtcag cggctgctcg
     6361 gcgctgacca gccaccgcgc cagcgacaag cgattttgtt gatcggcttt cggcagcggc
     6421 ggcaaaaacg ccgggacgtc ggcagtgact ttttcgccct gcttgtcgta agcgccgcgg
     6481 acgagaatga acgtgtcccg cggctgcggc atttcctgca tgaccatgct attggccacc
     6541 gcggcttggg cgtcggtctg gcgcttgcga agcgcggcca cgtcggcctt gagcttcttg
     6601 accgcctcgg catccggcgg cggatcctga gacgactcgg cggcggcgac cctggcctcc
     6661 gccgccttga tctcagccgc cagcgagtcg agctccgcct gctgctgcgg cgtgggaaag
     6721 ctcaagagcg ggacggcgtt gcccttccgg ccgtccagtc cgttttcggg caggttgttg
     6781 aaaaaggcgt acagctgata gaactcgcgc tgggtgagcg gatcgtattt gtggtcgtgg
     6841 cactgcgtgc aggcgaccgt cagtccgaga aacaccgtgg ccgtcgtgtt gacccgatcg
     6901 acgaggtagg cgttgaggta ttcctgaggg atggctccgc cctcgaaatt gatcatgtgg
     6961 ttgcggttga agccgctggc cagcttctgc cgcaggtcct gctcgcggtc gcccgtttcc
     7021 ggcagcagat cgccggcgat ttgctcgacg gtgaactggt cgtagggcac gttgcggttg
     7081 aacgactcga tgacgtattc ccgccagcgg ctcatgtcgc gaccagaatc gatgtggtag
     7141 ccgtgcgtgt cggcgaagcg
//
LOCUS       DASRJB010000848         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3628415, whole genome shotgun sequence.
ACCESSION   DASRJB010000848 DASRJB010000000
VERSION     DASRJB010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3628415"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..682)
                     /locus_tag="VFB80_22310"
     CDS             complement(116..682)
                     /locus_tag="VFB80_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36587.1"
                     /translation="MNDPLFPLLLALRYMHIFGAIALMGATIFMRFALVPAVAKLDPA
                     AKATLHEEVRSRWSKFVMAAAGLLLISGVLNLALTGRYNFDPIPGIPRGGYQMIVGIK
                     FLLSLPIFFIASVLAGRSDLAKRFQSSAKFWMNVNLTLAVLMVLIGGGLRFVGRTLKA
                     PATTPAPAAAIVPQDRPNASLRRRGEIE"
     gene            complement(704..2536)
                     /locus_tag="VFB80_22315"
     CDS             complement(704..2536)
                     /locus_tag="VFB80_22315"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN36588.1"
                     /translation="MSWFRIALRSIQQRGVASLLTMLSMALGVTLVVAVLSIDGVLAE
                     SFRNNASLGYNIIVGAKGGQEQLVLNTVFFLSRPIENVPYTYYLEFLKQPERDRLQRD
                     SYAARGAEVWRETAELQALTGNLGLSGAALDAVAAPIEQSLVPDMTLPRHPPVELKGR
                     HGKYGQMTELAIPLCLGDFYGTFRVVGTTPALFDDLVYDIERNRKFEFAQGRNFVWRS
                     PQHGYFEAVLGAKVASDIQLVELHLNVAGNAAARALVTERANWSRIDERDGALVVALG
                     QGTEESDVFLSLRRLQKDAEEGKRQLDDEQQQALTALLDPGVVLRRSQRNVTLGDAIA
                     PAHGEPGGHLHQRKFTVVGILKPSGTPNDRAVFINMEGFYLMEDHAKPVARPKPEEAA
                     EGPPLSESEQRAQMAEDRRRQQEVERAADPEPLPAELREVTAILLKVDVMFAPGIENY
                     INEGQQAQAALPVAVITRLFEFFVGPLKRVLLLLTVMICVVSAISILVSIYNSMSERR
                     GEIAVMRALGAGRGTVMGIILLEATLLALGGGVLGWLLGHAGVLIASPWIEEHTSVHV
                     GFLTWTTLPTEMLLIPGLIVLAIVVGVWPAISAYQTDVAKSLGK"
     gene            complement(2543..3229)
                     /locus_tag="VFB80_22320"
     CDS             complement(2543..3229)
                     /locus_tag="VFB80_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN36589.1"
                     /translation="MLQLRQVKKSYAEPDGGRLPILDIPLWEVKDGEQMVLVGKSGCG
                     KTTLLHTIAGITRADSGEISIDGINIARLSEAGVDRVRANKLGYVFQTFNLLAGFTAL
                     ENVLLGMTFGGGRRDPKRARHLLERVGLAHRTHHRPTALSVGEQQRVAVARALANRPS
                     LLLADEPTANIDPRNQQKIVDLIRETCREENVALIIVTHAPEVAGQFSRVDKLEEINL
                     VRADAPPLEK"
     gene            complement(3350..>3554)
                     /locus_tag="VFB80_22325"
     CDS             complement(3350..>3554)
                     /locus_tag="VFB80_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36590.1"
                     /translation="AGGGVKGGAVVGSSDRLGGYPASDPQTPENMAATIYQALGLPSA
                     AAWHDEQDRPHHVYHGEPIAGLT"
BASE COUNT          580 a         1185 c         1198 g          591 t
ORIGIN      
        1 tcggtcaggc gcaccgcgag caccgccttg gcctcgcgga gaacctcgat cgcttgggca
       61 cgctcgttca tggctggcca cgctcagaca aaaccgccgc agttcggctc cccgttcact
      121 ctatttcgcc gcggcggcga agggaagcgt ttgggcgatc ctgcggtacg atcgcggcag
      181 ccggagcagg cgtcgtcgcc ggtgccttca gggtgcggcc aacgaaccgc aagccgccgc
      241 cgatcaggac catgagcacg gccagcgtga gattcacatt catccagaac ttcgcgctgg
      301 actggaatcg cttggcgagg tcggagcgtc cggccaggac gctggcaatg aaaaagatcg
      361 gcagggacaa gagaaacttg atgccgacga tcatctggta gccgccgcgg ggaattcccg
      421 gtatcgggtc aaagttgtac cggccggtca gcgccagatt caaaacgccg ctaatcagca
      481 gcagccccgc ggcggccatc acgaatttcg accagcggct gcggacctct tcgtgcagcg
      541 tggccttcgc ggccggatcg agcttggcca cggcgggcac gagcgcaaat cgcatgaaga
      601 tcgtcgcccc catcagggcg atggccccga agatgtgcat gtagcggagg gccagaagca
      661 gcggaaagag cggatcgttc atggcaggtc agggcgagcg gtgttacttc ccgagcgatt
      721 tggccacgtc ggtctggtag gcgctgatcg cgggccagac gccgacgacg atggccagca
      781 cgatcaggcc ggggatcagc agcatttccg tgggcagcgt ggtccaggtg aggaagccga
      841 cgtgcacgct cgtatgttcc tcgatccacg ggctggcgat caagacgccg gcgtgcccca
      901 gcagccagcc gagcacgccg ccgcccaggg ccagcagcgt cgcttcgagc agaatgatgc
      961 ccatcacggt gccgcggcca gcgcccaagg cccgcatcac ggcgatctcg ccgcggcgct
     1021 cgctcatcga gttgtagatg ctgaccagga tgctgatcgc gctgacgacg cagatcatca
     1081 ccgtcaagag cagcagcacc cgcttgagcg ggccgacgaa gaactcgaac aagcgggtga
     1141 tcacggcgac gggcagcgcg gcctgggcct gctggccttc gttgatgtag ttttcgatcc
     1201 ccggtgcgaa catcacgtcc accttcagca ggatcgcggt cacttcgcgc agctcggccg
     1261 gcagcggctc gggatcagcg gcccgctcga cctcctgctg tcgcctccgg tcttcggcca
     1321 tctgcgcccg ctgctcgctc tcgctcagcg gcggtccctc cgcagcttcc tcgggcttgg
     1381 ggcgggccac cggcttggcg tggtcctcca tcagatagaa gccttccatg ttgatgaaca
     1441 cggcccggtc gttgggcgtg ccgctgggct tcagaatgcc gacgacggtg aatttccgct
     1501 ggtgcaaatg gccgcccggc tcgccgtggg ccggcgcgat ggcgtcgccc agcgtcacgt
     1561 tccgctgcga gcggcggagc acgacgcccg gatcgagcag cgccgtcaag gcctgctgct
     1621 gctcgtcgtc gagctggcgc ttgccctctt cggcgtcctt ctgcaagcgg cggagcgaga
     1681 ggaagacgtc gctttcctcg gtcccctggc ccagcgcgac gaccagcgcg ccgtcgcgct
     1741 cgtcgatccg ggaccagttg gcccgttcgg tcaccagcgc cctggcggcg gcattgcccg
     1801 cgacgttcaa gtgcagctcg acgagctgga tgtcgctcgc caccttagct cccagcaccg
     1861 cctcgaaata gccgtgctga gggctgcgcc acacgaagtt ccgcccctgg gcgaactcga
     1921 acttgcggtt ccgctcgatg tcgtacacaa ggtcgtcgaa cagcgcgggc gtcgtgccga
     1981 cgacgcgaaa cgtgccgtag aaatcgccca ggcagagcgg aatcgccagc tcagtcatct
     2041 gcccgtattt gccgtggcgg cccttgagct caaccggcgg atggcgcggc agcgtcatgt
     2101 cggggacgag cgattgctcg attggcgctg ccacggcatc tagcgccgcc ccgctcagtc
     2161 ccaggttgcc cgtgagcgct tgcagctcgg ccgtctcgcg ccagacctcc gcgccgcggg
     2221 ccgcatacga gtcgcgctgc aggcggtcgc gctccggctg tttgaggaat tccaagtagt
     2281 acgtgtaagg cacgttctcg atcggccggc tgagaaagaa gacggtgttg aggaccagct
     2341 gctcctggcc ccctttggcc ccgacgatga tgttgtagcc caggctggcg ttgttgcgaa
     2401 aagactcggc cagcacgccg tcgatcgaca gcacggccac taccagcgtc acgcccaggg
     2461 ccatcgacaa catcgtgagc agcgaggcca cgccgcgctg ctgaatgctg cgaagggcaa
     2521 tgcgaaacca actcatgggc ggctactttt cgagcggcgg ggcgtccgcg cggaccaggt
     2581 taatctcttc cagcttgtcc acgcggctga actggccggc gacttccggc gcgtgcgtga
     2641 cgatgatcag cgcgacgttt tcttcgcggc aggtctcgcg gatcagatcg acgatcttct
     2701 gctggttccg cgggtcgatg ttcgccgtcg gctcgtcggc caggagcagg ctgggccggt
     2761 tggccagggc ccgcgcgacg gccacccgct gctgctcccc caccgagagg gccgtcggcc
     2821 gatggtgggt gcgatgcgcc aggcccacgc gctccagcaa gtgccgggcc cgcttcggat
     2881 cgcgccggcc gccgccgaac gtcatgccga gcagcacgtt ttccagcgcg gtaaagccgg
     2941 ccagcaggtt gaacgtctga aagacgtagc ccaatttgtt cgcccgaacg cgatcgacgc
     3001 cggcttccga caggcgggcg atgttgatcc cgtcgatcga gatctcgccc gagtcggccc
     3061 gcgtgatccc ggcgatcgtg tgcagcagcg tcgtcttgcc gcagccgctc ttgcccacca
     3121 gcaccatctg ctcgccgtcc ttcacctccc agagcggaat gtcgaggatc ggcagccggc
     3181 cgccgtcggg ctccgcgtac gactttttca cctggcggag ctggagcatg acggagcgcg
     3241 aggtcggcgg aagggaagcc agatggtatg cgattcgccc ggttctgggc agggcagcat
     3301 cgcgggccgg tggcagctat acggcctggc ggcggccgaa gttcgagaac tacgtgagcc
     3361 ccgcgatggg ctcgccgtga tagacgtggt gcgggcggtc ctgctcgtcg tgccaggcgg
     3421 ccgcgcttgg caggccgagc gcctggtaga tcgtggcggc catgttctcc ggcgtctgcg
     3481 gatcggaagc gggatagccg ccgaggcggt cggacgagcc gacgaccgcg ccacccttca
     3541 cgccgccgcc ggcg
//
LOCUS       DASRJB010000849         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1378620, whole genome shotgun sequence.
ACCESSION   DASRJB010000849 DASRJB010000000
VERSION     DASRJB010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378620"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFB80_22330"
     CDS             <1..183
                     /locus_tag="VFB80_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36591.1"
                     /translation="PTSSTRARLKLTGHKVYKSGIVGLEYSIQPARPAAGRRQSPRAA
                     TKKKSRSPALRSRSKR"
     gene            complement(244..1674)
                     /locus_tag="VFB80_22335"
     CDS             complement(244..1674)
                     /locus_tag="VFB80_22335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN36592.1"
                     /translation="MSARRIPHFCACLVAAWSAGLVWTPAAAAQQERPNIVFILMDDL
                     RFDELGCMGHPFVQTPHIDRLAREGAMFSNAFATTPLCSPSRASFLTGLYPHTHGILD
                     NTDRSPRSHELVTWPRLLHDAGYETAFVGKWHMGVDDRPRPGFDHWVSVKGQGRYVDP
                     DLNVDGQPVKEKGYVTDLFNERAVAFLKRPRERPFCLYLAHKCVHPDLEQRPDGSVSD
                     PTAAKFLPAERHRRLYADAKVPRRANAFQPPRGKPALERRIEGLPPLSRETATDDETI
                     RNRLRLLLAAEEGVGQIRAALSETGQLDKTLIVFTSDHGYFYGEHCLSVERRLAYEEA
                     IRIPLLIRYPPLIKPGTRPASFALSIDLAPTLLQLAGVPLPKGLHGRSLVPALKGEAE
                     QPRTRFLIEHTSDNVFPRVQKMGYQAVRTERWKYIHYRDLENADELYDLASDPYELRN
                     VIGEAASREALAALQVELRRLNEQSP"
     gene            1869..>2183
                     /locus_tag="VFB80_22340"
     CDS             1869..>2183
                     /locus_tag="VFB80_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36593.1"
                     /translation="MGQHAPNETLAEGELVFRVMWPMEIPGYVIAGIGLVAAIALGAS
                     PALRQMLGIGLWVPLLTGAVALFFAEESFRISWFKMPRWIGVSKNGIRWYQSFRIHWH
                     PWS"
BASE COUNT          360 a          690 c          733 g          400 t
ORIGIN      
        1 cccacaagct ccacgcgggc ccggctgaag ctgacgggtc acaaggtcta caagtcggga
       61 atcgtcggcc tggagtacag catccagccc gctcgaccgg ctgccggccg ccgccagtca
      121 ccacgcgcgg cgaccaagaa aaagtcccgc tctcctgccc tgcgatcaag atcaaaacgc
      181 tgagacgcgc ggatactgac ttcttgcggc gctatagttg ggcgctagcc aaccatgcca
      241 ggtctagggt gattgctcgt tgagccgccg caactcgact tgcagggcgg cgagcgcctc
      301 gcgcgatgcg gcctcgccga tcacgtttcg cagctcgtag ggatcggagg ccaggtcgta
      361 caactcgtcg gcgttttcca ggtcgcgata gtgaatgtac ttccaccgct cggtcctgac
      421 ggcctgataa cccatcttct gcacccgcgg aaagacgttg tccgacgtgt gctcgatcag
      481 aaatctcgtc cgcggctgct ccgcctcgcc cttcagcgcc ggcacgagcg agcggccgtg
      541 aagccccttg ggcagcggta cgccggccaa ctgcaggagc gtcggcgcca ggtcgatgct
      601 cagggcgaag gaggcgggcc tggtgccggg cttgatcagc ggcggatagc gaatgagcag
      661 cggaatgcgg atcgcttctt cgtacgccag gcggcgctcg acgctgaggc aatgttcgcc
      721 atagaagtag ccgtggtcgc tggtgaagac aatcagcgtc ttgtcgagct gcccggtttc
      781 gctgagcgcg gcgcggattt ggccgacgcc ctcttcggct gccagcagca gccgcaggcg
      841 gttgcggatc gtctcgtcgt cggttgccgt ctctcgggag agcgggggca ggccttcgat
      901 ccggcgctcc agggccggct tgccccgcgg cggctgaaac gcattggcgc gccgcgggac
      961 cttcgcatcg gcatacagcc ggcggtggcg ttcggcgggc agaaacttcg cggcggtcgg
     1021 atcgctgacg gagccgtcgg gccgctgctc caggtcggga tggacgcact tgtgggccag
     1081 gtacaggcag aacggccttt cgcgcggacg cttcaggaac gccaccgccc gctcgttgaa
     1141 caggtcggtc acgtagccct tttccttcac tggctggcca tcgacgttca agtcgggatc
     1201 gacgtagcgg ccttgtcctt tgacgctgac ccagtgatcg aatcccggcc gcgggcggtc
     1261 atcgacgccc atgtgccact tgccgacgaa cgccgtttcg tatcccgcat cgtgcagcag
     1321 ccgcggccag gtgaccagct cgtggctgcg gggactgcgg tcggtgttgt cgaggatgcc
     1381 gtgcgtgtgc gggtacaggc cggtcagaaa cgaggcccgg ctgggcgagc agagcggcgt
     1441 ggtggcaaag gcattgctga acatcgcccc ttcgcgggcc aggcggtcga tgtgcggcgt
     1501 ttggacgaac ggatggccca tgcaacccag ctcatcgaac cgcaggtcgt ccatcaggat
     1561 aaagacgatg ttcgggcgct cctgctgagc agcagccgct ggcgtccaaa ccaggcccgc
     1621 cgaccaggcg gcgaccaggc aggcacagaa atgcgggatg cggcgggcgg acatgatcag
     1681 tgctcgcttc ggcggctatg cgttccaggt tcgcggcggc gtaccactgc cggcttcggc
     1741 cggaccagct taatggtttc gcccgctcgt cggcactgct acttgtactg aatatgccgg
     1801 gtgtcacgcg gcaagtcact ggcatgggac cggacgtcct atagttgcgc agagtggagc
     1861 agcatcctat gggacagcac gcgccgaacg aaactctcgc ggagggcgaa ctcgttttcc
     1921 gcgtcatgtg gccgatggag atacccggtt atgtcattgc gggcatcggg ctggtggccg
     1981 ccatcgccct cggtgcctcg cccgccttgc gccagatgct gggcatcgga ctttgggttc
     2041 ccttgctcac cggggcggtt gcgctgttct tcgccgaaga atcgttccgc atttcctggt
     2101 tcaagatgcc ccgctggatc ggcgtatcca aaaacggaat ccgctggtac cagagttttc
     2161 ggatccactg gcacccctgg agc
//
LOCUS       DASRJB010000850         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_541893, whole genome shotgun sequence.
ACCESSION   DASRJB010000850 DASRJB010000000
VERSION     DASRJB010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541893"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFB80_22345"
     CDS             <1..1238
                     /locus_tag="VFB80_22345"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN36594.1"
                     /translation="EGTHVEPGDIVVELDSSSLEQERTTQLISVTGQKALHVQAENKL
                     AAAQIARREYEFGIFVSEERLLIADAYVAQQTMSTAEAGLEQARRLFNRAIINAKQVE
                     ASIFAVEDARNRLIAAEAKLKNLREFTRDKMLKTFDSDIATASADVSAQEAKLTLEER
                     KLREIEEQIAKCTIRAPVAGEVVHANEYDSNNGNVQADFVVTAGAAVRERQPIIRIPS
                     SKEMQVRATVNQARVTLVKAGMPVTIRVDALKDQLIHGEVVKVNPYADPGSWSSGNIK
                     RYATFVRVPNPPPGLRSGMNAEVRIHIEQEPDALQVPVQALTQHEGRFYGLVQHGENY
                     ETREVTIRSTNDKVATIGSGLSQGDIVVMNPRRAGDLLDLPELPSGASQIAKAPRAAE
                     AEKPPSGEDANTTPGGGGE"
     gene            1241..2635
                     /locus_tag="VFB80_22350"
     CDS             1241..2635
                     /locus_tag="VFB80_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN36595.1"
                     /translation="MFLLRTLQLGLKSLLVHPMRSLLTVLGIFIGVASVIWLLAIGEG
                     ISQEAQRQIEGLGADNIIIRSVKPPTETLAAYSGPVPYGLKRDDYDLLVQTIPTLKAA
                     LPIREILRTFSFDGRNADGRLVGCTPDYFEVTRLELDRGRLLSDADIEAGHNFCVLSS
                     GIAERLFPYEDPIGHRVFLAETKDTYHVVGILKYRLPSAAIGGSLEAQDFNSDIYIPI
                     STLRQRAGDFAFTRRGNSRTFEIVELNQITLRVNSVADVRETANLVRSSLHVPPDSAR
                     QDPAQALAAAKADPNADGPPRFDIAVIVPEELLEQARLTRLTFMVLMGLIAAISLVVG
                     GIGIMNIMLATVTERTREIGIRRALGATRSHIILQFLVETVSLSIVGGTTGILGGLLC
                     PVIVVGVRDLLQKYAAHLLTGLPDVARRVEPQIVIASLPLAFCISVVVGVVFGLYPAY
                     RAAQMDPIEALRHE"
     gene            complement(3125..>3963)
                     /locus_tag="VFB80_22355"
     CDS             complement(3125..>3963)
                     /locus_tag="VFB80_22355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZN36596.1"
                     /translation="PNQGFLTPQWGDVDTFVIGDVNDFLSPPPPRLRSDEYAEAFEEV
                     FLFGGDGVTTRTIRTREQTEIGIYWGYDGRPGLGTPPRLYNQIARVIAKRKDNTVDEN
                     ARLFGLMNLALGDAGIQCWKTKYIYDFWRPIIGIRAGDDDGNRDTAGDPDWNPLGAPL
                     TNGPPGAPNFTPPFPACTSGHATFGAAALRVVGNFYGTDNISFSFVSDEFNGINADQN
                     GNVRPVVRRRFDSLREAVRENAQSRIYLGIHWQFDAQDGIRSGTEIADYVTSHALQPR
                     RR"
BASE COUNT          720 a         1343 c         1242 g          658 t
ORIGIN      
        1 ccgaagggac gcacgtcgag ccgggcgaca tcgtcgtcga gctcgactcc agttccctgg
       61 agcaggaacg caccacgcag ctcatctcgg tcaccgggca aaaggccctc cacgtgcagg
      121 ccgagaacaa actcgccgca gcccagatcg cccgccgcga atacgagttt gggatcttcg
      181 tgagcgaaga gcggctgctc atcgccgacg cgtacgtcgc ccagcaaacg atgagcaccg
      241 ccgaagccgg cctggagcag gcccgccggc tgttcaatcg tgcgatcatc aacgccaagc
      301 aggttgaggc gtcgatcttc gccgtcgagg acgcccgcaa ccgcctgatt gccgctgagg
      361 ccaagctgaa aaacctccgc gaattcaccc gcgataagat gctcaagacg ttcgacagcg
      421 acatcgccac cgcctcggcc gacgtttccg cccaagaggc caagctgacg ctcgaggagc
      481 ggaagctgag ggagatcgag gagcagatcg ccaagtgcac gatccgggcc ccggtcgccg
      541 gcgaggtcgt tcacgccaac gaatacgact cgaacaacgg caacgtccag gccgatttcg
      601 tcgtcacggc cggggccgct gtccgcgagc ggcagccgat cattcgcatt cccagctcca
      661 aggaaatgca ggtgcgggcc acggtgaacc aggcccgggt gacgctggtg aaggccggca
      721 tgcccgtgac gattcgcgtc gatgcgctca aggaccagtt gatccacggc gaagtggtca
      781 aggttaatcc atacgccgat ccaggcagct ggtcgagcgg caacatcaag cggtacgcca
      841 cgttcgtccg cgtgcccaat ccgccgccgg gactgcggag cggcatgaac gccgaggtgc
      901 ggattcacat cgagcaggag cccgacgcgc tgcaggttcc cgtgcaagcc ctgacgcagc
      961 acgaggggcg gttctacggc ctggtgcagc acggcgagaa ctacgagacc cgcgaggtga
     1021 caatccgctc gacgaacgac aaggtggcca caatcggcag cggcctctcc caaggggata
     1081 tcgtggtgat gaacccgcgc cgcgccggcg atctgctgga cctgcctgaa ttgccgtccg
     1141 gcgcaagtca gatcgcgaag gccccccgcg ccgccgaagc cgaaaagccc ccgagcggcg
     1201 aagacgcgaa caccacgccc ggcggcggcg gcgaataacg atgttccttc tccgaacgtt
     1261 gcagctcggc ctgaagagcc tgctggtgca tccgatgcgg tcgctgctga ccgtgctcgg
     1321 catcttcatc ggcgtggcca gcgtcatttg gcttttggcc atcggcgaag gcatcagcca
     1381 ggaagcccag cggcagatcg agggtttggg cgccgacaac atcatcatcc gctccgtcaa
     1441 gccgccgact gagacgctgg cggcctacag cggcccggtg ccctatggcc tcaagcgcga
     1501 cgactatgac ctgctggtgc agaccattcc cacgctcaag gccgcgctgc cgatccgcga
     1561 aatcctccgc accttctcat tcgacggccg gaacgccgac ggccgactcg tcggttgcac
     1621 acccgactat ttcgaagtca cgcggctgga gctcgaccgc ggccgcctgc tcagcgacgc
     1681 ggacattgaa gcgggccaca atttctgcgt gctctccagc ggcatcgccg agcggctgtt
     1741 cccctatgaa gacccgatcg gccaccgcgt gttcctggcg gagaccaagg acacgtacca
     1801 cgtcgtcggc atcctcaagt accgccttcc ctcggcggcg atcggcggct cgctggaggc
     1861 ccaggacttc aacagcgaca tctacatccc gatcagcacg ctgcggcagc gggccggcga
     1921 cttcgccttc acccgccgcg gcaactcccg cacgttcgag atcgtcgagc tgaaccagat
     1981 cacgctccgc gtcaattcgg tggcggacgt gcgggaaacg gcgaacctcg tccgcagctc
     2041 gctgcacgtg ccgcccgatt cggcccggca ggatcccgcc caggccctgg ccgccgccaa
     2101 ggccgacccc aacgccgacg ggccgccccg attcgacatc gccgtcatcg tgcccgaaga
     2161 gctgctggaa caggcccgcc tgacgcggct gacgttcatg gtgctgatgg ggctgatcgc
     2221 cgccatctcg ctggtcgtcg gcggcatcgg cattatgaac atcatgctgg ccacggtgac
     2281 cgagcggacc cgcgagatcg gcatccgccg ggcgctcggt gcaacgcggt cgcacatcat
     2341 tctgcagttc ctggtcgaga ccgtctcgct gtcgatcgtc ggcggcacga cgggaatcct
     2401 ggggggcctg ttgtgcccgg tgatcgtggt cggcgttcgc gacctgctgc aaaagtacgc
     2461 cgcccacctg ctcaccggcc tgcccgacgt ggcccgccgc gtggagccgc agatcgtcat
     2521 tgcctcgctg ccgctggcgt tctgcatctc cgtcgtcgtc ggcgtcgtgt tcgggctcta
     2581 tcccgcctac cgcgccgccc agatggaccc gatcgaagcg ctgcggcacg agtagctcgc
     2641 gggccacaag tgccgccccc cgaaattggt gctctgaact tgccttttgt cgccaattct
     2701 cgccacaagg ccttgcaggc cagtgacttg cgacgggagt ctcgaaaagc ccttaacaac
     2761 tacttattag cctcagagac gggctgtttt ttagcgcgcc ggccggtctg tcacccttga
     2821 tccggcgcaa ccaaccggac gaacctgcgc aacccccgag tgccgccggg cacgccagag
     2881 ttcggccaat tgggagactc tatccacaag tggacacaat tatccgcagc cgcccgaggt
     2941 ctgcaagcaa cttgtgcaaa ttttgtccac cacatgccaa cttacgtcga tcccccgcgg
     3001 cgcaactcta ttgcccgccg caacttccgc ctccgcgccg gcgcgcaact ttttttcgcg
     3061 ctgcccggcg gaatggacta gtgtggtcca ttccgcgcgg tcgcccaatc cagacccaag
     3121 ccaatcaccg ccgccggggc tgcagggcgt gtgacgtgac gtagtcggcg atctcggttc
     3181 ccgatcggat gccgtcctgg gcgtcgaatt gccagtggat tcccagatag atgcggcttt
     3241 gggcattttc gcgaactgcc tcgcgcaaag aatcgaaacg ccgccgcacg accggccgca
     3301 cgttgccgtt ctggtcggcg ttgatgccgt tgaattcgtc cgaaacgaac gagaacgata
     3361 tgttgtccgt gccgtagaaa ttgccgacga cccgcagcgc cgcagcgccg aaggtggcgt
     3421 gaccggaagt gcacgcgggg aagggcggcg tgaagttcgg tgcgccgggt gggccgttgg
     3481 tgagcggcgc gccgagcgga ttccagtcgg gatcgcccgc agtatcgcgg ttgccgtcgt
     3541 cgtcgcccgc gcgaatgccg atgattgggc gccagaaatc gtagatgtac ttggtcttcc
     3601 agcactgaat gccggcatcg cccagggcca gattcatcag gccgaacaga cgggcgttct
     3661 cgtcgaccgt attgtccttg cgcttggcaa tgacgcgggc aatctggttg tacagccgcg
     3721 gcggcgtgcc cagccccggg cggccgtcgt agccccagta gatgccgatc tcggtctgct
     3781 cgcgggtgcg gatcgtgcgc gtggtgacgc cgtcgccgcc gaacagaaag acttcttcga
     3841 aagcttcggc atactcgtcg gaccgcagac gtgggggcgg cggcgaaagg aagtcgttca
     3901 cgtcgccgat gacgaacgta tccacgtcgc cccactgcgg cgtcaggaag ccctggttcg
     3961 ggt
//
LOCUS       DASRJB010000851         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3916702, whole genome shotgun sequence.
ACCESSION   DASRJB010000851 DASRJB010000000
VERSION     DASRJB010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3916702"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VFB80_22360"
     CDS             complement(<1..2207)
                     /locus_tag="VFB80_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN36597.1"
                     /translation="MADPLTSAASRPVALRMRADLVARRQRWQGREYWTVKDPLALRY
                     YRFEEEEFAILNMLDGQTSVDQIRERFESQFAPQRLSAAQLQHLLSMLHQSHLLVADA
                     PGQGGQLQLRDRQRQRRQWLAALANPLAIRFRGVDPDRFLAWLNTRCGWLFSLPAAAA
                     ALLLALAALLLVAAEFDVFCSRLPAFQAFFAVNNWLWLAAVLLVTKVIHEIGHGLACK
                     RFGGECHEMGLMLLVFTPCLYCNVSDAWMLKSRWRRAAIGAAGMYVELILASLATLVW
                     WFSEPGLVNHLALNVMFVCSVSTLLFNANPLMRFDGYYILADLLEIPNLRQKSAAVIQ
                     RKLGAWLLGVRERPDPFLPARRRWLFAAYSLASAIYGWLVAVSIFWFLYRVLEPYGLK
                     VVGQLLGLAMIASLIVVPLVRLCHFLLQPARVQDVNKLRAAISLSAIAAVAGIILCVP
                     LPYYVACTFEVQPRDGQSVYVDVPGELREVHVRGGAVAAGQPLARLDDVELRLAEQRL
                     FSQRSDLAVRIAGIRQRAHSDDQALLELAQTQEALAALDRQIARLQVDLAKLTIRAPA
                     AGVIVPPPARSDRSPAKLKLASWTGRPLEARNVGAYLETSTLVCRIAQPSQFEAILAI
                     GQEELDFVQPGQRVDLLLNSAPGERRSGTIDRLAEENMQAAPARLSEQAGGGLATHSG
                     ADGIERPRSVVYQASVPLDDDSGCIVAGTTGSARVHAGSQPLWQRLWRSACRT"
     gene            complement(2231..3145)
                     /locus_tag="VFB80_22365"
     CDS             complement(2231..3145)
                     /locus_tag="VFB80_22365"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN36598.1"
                     /translation="MTTLAITLLALAAPPLVSATPAPGKQVQLAHCLVSLLADVQVPA
                     REAGALTAVEVTEGQLVTQGQLLARLDDRQPQLDKLAAELQRDAALAKAQDDIEVRFA
                     VAAQAVAAAELERSLAIERRSPGGVTQQEIQKLQLARHRDELQIERSRLEMRVAKMNA
                     DVHQASVQSAEDAVARRQIVSPLDGVVVALLHERGEWVAAGEAVVQVVRVDRLRVEGL
                     LSTAEYSPEEIAKRPVTVDVPLAGGRVGRFAGQIVFISPLVAAGNTYRVRAEVENRVE
                     AGQPLLRPGMTATMTIDVGTVHAVGIPR"
BASE COUNT          505 a         1125 c         1108 g          454 t
ORIGIN      
        1 aacgtccggc atgcgctccg ccacagccgc tgccacagcg gctgcgagcc ggcgtggacg
       61 cgcgccgagc ccgtcgtgcc ggcgacgatg cagccggagt cgtcgtcgag cgggacgctg
      121 gcctggtaga cgaccgaccg cggccgctcg atgccgtccg cgccgctgtg cgtcgccagg
      181 ccgccgccgg cctgctccga gagccgcgcc ggcgcggcct gcatgttctc ctcggcgagg
      241 cggtcgatcg tgccgctgcg gcgctcgccc ggcgcggagt tgagcagcag atcgacccgc
      301 tgtcccggct gcacgaaatc gagctcctcc tggccgatcg ccaggatcgc ctcgaactgg
      361 ctcggctggg cgatccggca gaccagcgtg ctcgtttcca gatacgcgcc gacgttgcgg
      421 gcctccaacg gccggccggt ccagctggcc agcttcagct tcgccggcga gcgatcgctg
      481 cgtgcgggcg gcggcacgat cactccggcg gccggcgcgc ggatcgtcag ctttgccaga
      541 tcgacttgca gcctggcaat ttgccggtcg agcgcggcca gggcctcctg cgtctgggcc
      601 agctccaaga gcgcctgatc gtcggagtgg gcccgctgcc ggatgccggc gatccgcacc
      661 gccaggtcac tgcgttgcga aaagaggcgc tgctcggcca gccgcagctc gacgtcgtcc
      721 agccgggcca gcggctgtcc cgcggcgacc gcaccgccgc ggacgtgcac ctcgcgcagc
      781 tcccccggca catcgacata gacgctctgc ccgtcccgcg gctgcacctc gaacgtgcag
      841 gccacgtaat acggcagcgg cacgcacagg atgattccgg cgacggcggc gatcgccgag
      901 aggctgatgg ctgcccgcag cttgttcacg tcttggaccc tcgccggctg aaggaggaag
      961 tggcacagcc gcaccagcgg cacgacgatc aggctcgcga tcatcgccag gcccagcagc
     1021 tggccgacga ccttcagacc atacggttcc agcacgcggt acaggaacca gaagatcgac
     1081 accgccacga gccagccgta gatcgccgac gccaggctgt aggcggcgaa gagccagcgg
     1141 cggcgtgcgg gcagaaaggg atcgggccgc tctctcacgc cgagcagcca cgcgccgagc
     1201 ttgcgctgta tcacggccgc cgatttctgc cgcaggttcg ggatctcgag caggtccgcg
     1261 aggatgtagt aaccgtcgaa ccgcatcagc gggttggcgt tgaagagcag cgtgctgacc
     1321 gagcagacga acatcacatt gagagcgagg tgattgacca gccccggctc gctgaaccac
     1381 cagaccagcg tcgccagcga cgcgagaatc agctccacgt acattcccgc cgcgccgatc
     1441 gccgcccgcc gccagcggct tttgagcatc caggcgtcgc tgacgttgca gtacaggcag
     1501 ggcgtgaaga ccagcagcat caggcccatt tcgtggcatt cgccgccgaa ccgtttgcag
     1561 gccaggccgt ggccgatctc gtggatgacc ttcgtgacga gaagcacggc cgccagccag
     1621 agccagttat tgacggcgaa gaacgcttga aaggccggca gccgcgagca gaagacgtcg
     1681 aactcggccg cgaccagcag cagggccgcc agcgccagca gcagggccgc cgcagccgct
     1741 ggcaacgaga acagccagcc gcagcgcgta ttgagccagg ccagaaaccg gtcgggatcg
     1801 acgccgcgaa agcggatggc cagcgggttg gcgagggcgg cgagccactg ccgccgctgc
     1861 cgctggcgat cgcgcagctg cagctgcccg ccctggcccg gcgcgtcggc cacgagcagg
     1921 tggctctggt gcagcatcga cagcagatgc tgaagctgcg ccgccgagag tcgctgcggg
     1981 gcgaattgcg attcaaagcg ctcgcgaatc tggtccacgc tcgtctgccc gtcgagcatg
     2041 ttcaggatcg cgaactcttc ctcctcgaag cgatagtagc gcagcgcgag cgggtccttg
     2101 accgtccagt attcgcgccc ctgccaccgc tgccgccgcg ccacgagatc ggcccgcatg
     2161 cggagggcca ctggacggct ggcggcggag gtcaagggat cggccatcgg cgtcgcaagc
     2221 gggcctgcaa ttatcgggga attcccaccg catgaaccgt tcccacgtcg atcgtcatcg
     2281 tggccgtcat gccgggccgc agcagtggct ggccggcttc gacgcggttc tcgacttcgg
     2341 cccgcacgcg gtaggtgttg ccggcggcga ccagcgggct gatgaacacg atttggccgg
     2401 cgaagcggcc gacgcggccg ccagccagcg gcacatcgac ggtcaccggg cgtttggcga
     2461 tctcctccgg gctgtactcg gccgtgctga gcagcccttc gacacggagg cgatcgacgc
     2521 ggacgacctg cacgaccgct tcgccggcgg cgacccactc gccccgctcg tgcagcagcg
     2581 ccacgacaac gccgtcgagc ggcgaaacga tctgccggcg ggcgacggcg tcctcggcgc
     2641 tctggaccga ggcctggtgc acgtcggcgt tcatcttggc gacccgcatc tcgagcctgc
     2701 tccgctcgat ctgcagctcg tcgcgatgcc gggcgagctg gagcttctgg atttcctgct
     2761 gcgtcacgcc gccggggctg cggcgctcga tcgccaggga ccgttcgagc tcggctgcgg
     2821 ccaccgcctg ggccgccacc gcgaaccgca cttcgatgtc gtcctgggcc ttggccagcg
     2881 cggcgtcgcg ctgcaattcg gccgccagct tgtcgagctg cggctgccgg tcgtcgaggc
     2941 gggccagcag ctgcccctgc gtgacgagct gcccctcggt cacttcgacg gcggtcaaag
     3001 cgccggcctc ccgcgccggc acctgcacgt cggccagcag cgacacgagg cagtgcgcga
     3061 gttggacctg ctttcccggc gcgggcgtcg cggacacgag cggcggagcg gcgagcgcca
     3121 ggagcgtgat ggcgagagtg gtcatggttt cgtagcacaa gcgtctggct tgtgcgggcg
     3181 cgagcaagcg ag
//
LOCUS       DASRJB010000852         5215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2774970, whole genome shotgun sequence.
ACCESSION   DASRJB010000852 DASRJB010000000
VERSION     DASRJB010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774970"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFB80_22370"
     CDS             <1..272
                     /locus_tag="VFB80_22370"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZN36599.1"
                     /translation="LPVAWAAVAASVLFFLLSNFGVWLAGDGSGQARMYPLTPAGLLA
                     CYIAAIPFSLNTLYGNLLFSGVLFGGLEFYKSTVAHRAEKSAIAT"
     gene            331..1023
                     /locus_tag="VFB80_22375"
     CDS             331..1023
                     /locus_tag="VFB80_22375"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HZN36600.1"
                     /translation="MPAPLPNPLPKGEGTKLCCSWSGGKDSCLALHRALAAGGELRCL
                     LTMFTEDRERSRSHGLSRAVLEAQAAAIGVPLLSAAATWDDYEITFIRLLDDAREAGA
                     EMCVFGDIDIPRHREWEENVCRRAKLTASLPLWQEDRFQLLEEWWAAGFEARIIVARE
                     GLVDRRYLGRALGRQVAQELAATGVDPCGENGEFHTVVTGGPLFARPLALELRGQVLR
                     SGCWFQDVAVTM"
     gene            1100..2101
                     /locus_tag="VFB80_22380"
     CDS             1100..2101
                     /locus_tag="VFB80_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HZN36601.1"
                     /translation="MKAAYIENTGPAEATIQYADVPKPEAAGTQVLVKVGAVSVNPID
                     TYLRSGANYWPLPKPFIIGCDLAGTVEAVGPQARRYKVGDRVWGSNQGLLGRQGTFAE
                     YCAPEEQWLYPTPANVMDEQAAACALVGITAHLGLFRHAQLQAGETVFVNGGTGGVGS
                     LVVQMAKAAGAKVVATAGSPEKVAALRQLGADVALNYKSDDIAAAVKEFAPEGVNVYW
                     ETVREPDFDKIVSYLGERGRMVLMAGRDARPAFPVGPFYVKGCSLHGFVIFKATPGEQ
                     RAAADDISRWLAQGKLKANISRVLPLSEAAAAHQLQEDNTLRKAGTLAGKIVLKPQP"
     gene            complement(2143..3315)
                     /gene="larC"
                     /locus_tag="VFB80_22385"
     CDS             complement(2143..3315)
                     /gene="larC"
                     /locus_tag="VFB80_22385"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZN36602.1"
                     /translation="MKIAYFDCLSGISGDMTLGALVDCGVELATLQAGIDSLGFPNIK
                     LASEEVKRRGFRAAKVKVLHEPEHAHRHLHHITEKIDASSVLTPPQKDLARRIFTCIG
                     EAEAKVHGTTIRKVHFHEVGAVDSIADIVGCAVGLSLLAVDRIVCSPVPTGTGHIEIE
                     HGRVSVPAPATAEILRGVPLAPSAVPFELTTPTGAAIVKTVADEFGPLPAMTIQTIGL
                     GAGDRDLKEQPNVLRLILGEAAGGMATDQIWVLETNLDDISGEIIGHTTARLFEAGAL
                     DVYTTSIQMKKNRPGVLLSVLCPAELLAKMEKIIFKETSTLGIRRWQVARHKLERRPH
                     TIETPLGPIEGKLAVLPDGGTSFSPEFEACRRIATEKNVPLKDVYEAAMRASGGSS"
     gene            complement(3387..3746)
                     /gene="queF"
                     /locus_tag="VFB80_22390"
     CDS             complement(3387..3746)
                     /gene="queF"
                     /locus_tag="VFB80_22390"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZN36603.1"
                     /translation="MPAVPTSALETFENQFPGRDYTIEIVCPEFTSVCPKTGQPDFGT
                     LTITYTPDRKCVELKSLKLYLQGFRNEGIFYEHVTNRILDDLVAALAPRRLKLVASFT
                     PRGGISTSVTVTHENSK"
     gene            4064..>5215
                     /locus_tag="VFB80_22395"
     CDS             4064..>5215
                     /locus_tag="VFB80_22395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZN36604.1"
                     /translation="MSTAIQSDLAAYCREAAARAKAAAAALAQVRGEQKNAWLRHSAA
                     RLREQTAQLLAANADDIAAAPGFGLSDAAIDRLRLDPQRIDEIALGLEQVAALPDPVG
                     EVIESSVRPNGLQILKTRVPLGVVFFIYESRPNVTADAAAICLKSGNAVILRGGKEAA
                     HSSREIVEILKSTAAEHDLPADALHLVESPDRAAVGHFLALPQYIDVAIPRGGESLIR
                     RVAAEAQMPVIKHFTGNCHVYVDGSADLAMAERITINAKCQRTGVCNACESLLVHAAV
                     AAVFLPRIGSALAERGIEMRADERSRKQLGVPGVKPATEEDYATEFLGPIISIAVVEG
                     LDAAIAHINRYGSQHTDAIVTSDLAAARQFAAEVDSSAVMINASTRFNDG"
BASE COUNT          880 a         1634 c         1768 g          933 t
ORIGIN      
        1 tgctgccggt tgcttgggcc gcggtcgccg cgtcggtcct gtttttcctg ctctcgaact
       61 ttggcgtgtg gctggcgggc gacggctccg gccaggcccg aatgtatccg ctgacgccgg
      121 ccggcctgct cgcctgctac atcgcggcca ttccgttctc actcaacaca ctctacggca
      181 acctgctctt ttccggcgtg ctgttcggcg gcctggagtt ctacaagtcc accgtcgccc
      241 accgcgcgga aaagtcggct attgcgacgt gaggcggtga ctctctctct gggagagggc
      301 cggggtgagg gtctgagatc tcaatgactt atgccagccc ctctccctaa ccctctccca
      361 aagggagagg gaacgaaact ctgctgctcc tggagcggcg gcaaggattc gtgtttggcc
      421 ctgcaccggg cgctggcagc cggcggcgaa ctcaggtgcc tgctgaccat gttcacggaa
      481 gaccgcgagc gctcgcggtc gcacggcctg tcgcgggcgg tgctcgaagc gcaagccgcg
      541 gcgatcggcg tgcctctgct gtcggcggca gctacgtggg acgattacga aatcacgttc
      601 atccgcttgc ttgacgatgc ccgagaggcg ggcgcggaaa tgtgcgtctt cggcgacatc
      661 gacattccgc ggcaccgcga gtgggaggaa aatgtctgcc gccgggcaaa gctgaccgcg
      721 tcgcttccct tgtggcagga agatcgtttc caattgctcg aggagtggtg ggctgcaggc
      781 ttcgaggcgc gaattatcgt ggctcgcgaa ggactcgtcg atcggcgcta tctgggacgc
      841 gccctcggtc ggcaagtggc ccaggagctg gccgcgaccg gcgtcgatcc ctgcggcgag
      901 aacggcgaat tccacacggt cgtgaccggc gggccgcttt tcgcgaggcc gctggcgctg
      961 gaactccgcg gccaggtgct ccgcagcgga tgctggttcc aggacgtggc ggttaccatg
     1021 tagggtgcat gagcggagcg caatgcaccg cccgtgaaag tgcattgcgc tgcgattatg
     1081 caccctacga gaggacccga tgaaagccgc ctacatcgag aacacgggac cggccgaagc
     1141 gacgatccaa tacgccgatg tgcccaagcc tgaggcggcc ggcacgcagg tgctggtcaa
     1201 ggtcggcgcg gtgtcggtga atccgatcga cacctacttg cggagcggcg caaactattg
     1261 gccgctgccc aagccgttca tcatcggctg cgatctggcc ggcacggtcg aagccgtcgg
     1321 cccgcaggcg cggcgataca aggtgggcga ccgcgtctgg ggcagcaatc aaggtctgct
     1381 gggccggcag ggcacgtttg cggaatactg cgccccggaa gagcagtggc tctatccaac
     1441 gccggccaac gtgatggatg aacaagctgc ggcctgcgcg ctggtcggga tcacggcgca
     1501 cctgggcctg ttccgccacg cgcagcttca ggcaggcgaa accgtgttcg tgaacggagg
     1561 caccggaggt gtcggctcgc tcgtcgtgca gatggccaag gcggccggag cgaaggtcgt
     1621 tgccacggcg ggcagcccgg agaaagtggc ggcccttagg cagctgggcg cggacgtggc
     1681 gctgaactac aagagcgacg acatcgcggc ggccgtcaag gagttcgctc cggagggcgt
     1741 gaatgtctat tgggaaacgg tccgcgagcc ggatttcgac aagatcgtct cgtatctggg
     1801 cgagcggggc cggatggtgc tgatggctgg ccgcgacgcc cgcccggcgt tccccgtggg
     1861 gccgttctat gtgaaaggct gctcgctaca tggttttgtg attttcaaag ccactcccgg
     1921 cgagcagcgg gccgcggccg acgacatcag ccgctggctc gcgcagggca agcttaaggc
     1981 caacatcagc cgcgtcctgc cgctcagcga ggcggccgcc gcccatcagc tccaggagga
     2041 caacacgctc cgcaaagccg gcacgctcgc cgggaagatc gtgctgaagc cgcagccgta
     2101 gccgacgctg ccagcgtcgg cggccgatgc tggcagcttc ggctacgaag atccgccaga
     2161 cgcccgcata gcggcctcgt aaacgtcctt cagcggcacg tttttctcgg tggcgatcct
     2221 ccggcaggct tcgaactcag gcgagaagct cgtgccgccg tcgggcagca cggccagctt
     2281 gccttcgatc ggccccagcg gcgtctcgat cgtgtgcggc cgccgctcga gcttgtgccg
     2341 ggcgacctgc caacggcgga tgccgagtgt gctcgtctcc ttgaagatga tcttctccat
     2401 cttggccagc agctcggccg ggcagagcac cgagagcagc acgcctggcc ggttcttctt
     2461 catctggatg ctggtcgtat agacgtcgag cgcgccggcc tcgaacagcc gggccgtggt
     2521 gtggccgata atctcgccgc tgatgtcgtc gagattggtc tccagcaccc agatctgatc
     2581 ggtcgccatt ccgccggccg cttcgccgag gatcagccgc agcacgttcg gctgctcctt
     2641 caggtcgcgg tcgccggctc ccaggccgat ggtctggatc gtcatggcgg ggagcgggcc
     2701 gaactcgtcg gcgacggtct tgacgatcgc cgcgccggtc ggcgtcgtca gctcaaacgg
     2761 cacggccgac ggcgcgagcg gcacgccccg caggatctcg gccgtcgccg gcgcgggcac
     2821 gctcacgcgg ccgtgctcga tctcgatgtg gcctgtgccg gtcggcacgg gtgagcagac
     2881 gatccgatcg acggccagga gcgacaggcc gactgcgcag ccgacgatgt cggcgatcga
     2941 atcgaccgcg ccgacttcgt ggaagtgcac tttgcggatc gtcgtgccgt ggaccttggc
     3001 ctcggcctcg ccgatgcagg tgaagattcg gcgggcgagg tccttttgcg gcggcgtcag
     3061 gacgctgctg gcgtcgatct tttcagtgat gtggtgcagg tgccggtggg catgctccgg
     3121 ctcgtgcagc accttcacct tcgctgcgcg aaagccgcgg cgcttcacct cttcgctggc
     3181 cagcttgatg ttggggaagc ctagcgaatc gatccccgcc tggagcgtcg ccagctcgac
     3241 gccgcaatcg acgagcgccc ccagcgtcat atcgccgctg atgccgctca aacagtcgaa
     3301 gtaggcaatc ttcatggaaa tcaaaattcc aagaaccgaa gaaccaaatc ctctagggat
     3361 gcacccttgg atcttcggtt cttaggttat ttgctgtttt catgcgtcac cgtcacgctc
     3421 gtgctgattc cacccctcgg cgtaaagctg gcgacgagct tgaggcggcg gggcgcgagg
     3481 gcggcgacga ggtcgtcgag gatgcggttg gtaacgtgct cgtagaagat gccttcgttg
     3541 cggaagcctt ggaggtagag cttcaggctc ttcagttcga cgcacttgcg gtcgggagtg
     3601 tacgtaatcg tcagcgtgcc gaaatccggc tggcccgtct tcgggcagac cgacgtaaac
     3661 tctggacaga cgatttcgat ggtgtagtcc cggccgggaa actggttctc aaaggtttcc
     3721 agggcggagg tgggcacggc gggcatcagt tcgtccttcg gcgcagagcc gattgtgccg
     3781 cgccgcgcga cgagcggcta ggcccgccag ggccgctgga atgctggcgt attgcctacg
     3841 cgctgacctt aaaccgtttc tgcggtagag gttacgacga attaccagtt tccagaagca
     3901 cattagcgat agaatcttgc tgcagcacca agcaccccag ccgccgacgt tgaccggctt
     3961 cgcgaattga aagatactcc gatcggggac gctgtcgcgt gccgaaagtc cttggcgtga
     4021 accggcttgc gccgtttttg tcgcttacgt cgggatagtg gaaatgagca ccgccatcca
     4081 gagcgatctg gctgcctatt gccgcgaggc ggcggcccgc gccaaggctg cggctgcagc
     4141 attggcgcag gtccgtggcg agcagaagaa tgcctggctg cggcactcgg cggccaggct
     4201 tcgcgaacag acggcacaac tcctggcggc caatgccgac gatatcgcgg cagctcccgg
     4261 ttttgggttg agcgacgcgg cgatcgaccg cctgcgactg gacccccagc ggatcgacga
     4321 aatcgcctta ggtttggagc aggtcgcggc gctacctgat ccggtgggcg aggtgatcga
     4381 atcgtcggtg cggcccaacg ggttgcaaat cctcaaaacc cgcgtgccgc taggagtcgt
     4441 gttcttcatc tacgagtccc ggcccaacgt cacggccgat gcggcggcga tttgcctcaa
     4501 gagcggcaac gcggtgatcc tgcgcggcgg caaggaagcg gctcattcca gccgggagat
     4561 cgtcgagatc ctgaagagca ccgcagccga gcacgacctg ccggcagacg cactgcacct
     4621 ggtcgagtcg ccggatcggg ctgccgtcgg gcattttctg gccctgccgc agtacatcga
     4681 cgtggcgatt ccgcgcggtg gcgagagcct catccgccgg gtcgccgccg aggcccagat
     4741 gccggtgatc aagcatttca cgggcaattg ccacgtttac gtcgatggct cggccgacct
     4801 ggcgatggcc gaacggatca cgatcaacgc caagtgccag cggacgggcg tctgcaacgc
     4861 ctgcgaatcg ctgctggtgc acgccgccgt cgccgccgta ttcctgccgc gaatcggctc
     4921 ggcgctcgcc gagcggggca ttgagatgcg ggccgacgag cggtcccgta agcagctagg
     4981 tgtgccgggc gttaagcctg cgacggaaga agattatgcg accgaattcc tgggaccgat
     5041 tatctcgata gcggtcgtcg agggcctgga cgcggcgatc gcgcacatca accgctacgg
     5101 ctcgcagcac accgacgcga tcgtcaccag cgacctggcc gccgcgcggc agtttgcggc
     5161 tgaggtcgat agctcggcgg tgatgatcaa cgccagtacg cggttcaacg acggc
//
LOCUS       DASRJB010000853        12170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3355843, whole genome shotgun sequence.
ACCESSION   DASRJB010000853 DASRJB010000000
VERSION     DASRJB010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3355843"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1413
                     /gene="corA"
                     /locus_tag="VFB80_22400"
     CDS             286..1413
                     /gene="corA"
                     /locus_tag="VFB80_22400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZN36605.1"
                     /translation="MSRKNRRKPPPREIKVHRRTEPGSSPGQVLADPAQPQPVMHVIA
                     YGASGVVEAEVKTADEAHALVGKQPMTWINVEGLGDAETIERFGELFGLHRLALEDTV
                     NVHQRAKVEDYGEVLFIVLRMVHCGNLADSRCGTEQISLFLGPNWLLTFQEGHPGDSF
                     DRVRARIREKGGRMRTLGGDYLAYALIDATIDNYYPVLEVYAERLDELEEQVLNPRSV
                     RAVDHLHEVKADLLVLRRAIWPLRDAMAYLSREETARFTEATRPYLRDCYDHIVQVVE
                     LVETYRELTADLRDLYMSSLSNRINETMRVLTIISTLFIPLTFIAGIYGMNFDPDTSP
                     WNMPELRWYLGYPLALVAMVAIGVGMLIYFVRQGWLFRGKG"
     gene            1636..2844
                     /locus_tag="VFB80_22405"
     CDS             1636..2844
                     /locus_tag="VFB80_22405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN36606.1"
                     /translation="MNAIYWNRIGRWAAGLTAGAALAIAGPAWSQQPPTPAPAPKAPV
                     TPKQDPVKPAADPAREAARDRVEGAREAARDRVEGAREGAQDARRDAREGAQDARRDI
                     RDARGDLREARRDARQALRAADLGLWFGARAGTTGLVVADVATQGAIARAGFLEGDRL
                     ISVNGTPVTTEAEFVRLFTDDAIRDQQVKVVVMRGGKEQVIVVQPSVLIQEAVAYDPL
                     WQYGIILDDRTPDRLVIQRVFPRTPAFYAGLRSGDVIVSARGQRLAAAADLLRALSAN
                     TDRLALQINRANAMRDVQLESSSNFGADVQTLLRPRIDDPNRPATPADTQPRLDAPRP
                     GTPAVPPRPGTTPATPAVPGRPATPPTTTTPALPAVPPIPATPPAAPAPTAPAAPAAP
                     APAPAAPSAP"
     gene            2962..3561
                     /locus_tag="VFB80_22410"
     CDS             2962..3561
                     /locus_tag="VFB80_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36607.1"
                     /translation="MKSRSTDQRLIRPPQFGLRTLLLVVTACSVLFALGHWLSPIAFV
                     GLLLLVASITLHVAGNAIGTRLRELGDRPPPDDAAANRAGEPRPLEFASATRLSERQG
                     LGWVIYAATIAGTLAGGIGGGVWTWLTSREPAGLLQLAVGVMAFAVLGGLAAFAAAAF
                     TQVLLGALWQALHPLPAARPTSLASADGARDNPDLGHSN"
     gene            3580..4629
                     /locus_tag="VFB80_22415"
     CDS             3580..4629
                     /locus_tag="VFB80_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36608.1"
                     /translation="MRRLILILAAFIGLSLVAAPAQAWWGCHWRHCGWGYRGCGWGGY
                     CGYRGYGWGGYGNYCGFGPAYYYPAYNYPLINYGGYGYGCGYGYGGLNYGTPISIGYY
                     ANRGGYYNANSNFALAALPQPRTPILTNVDAQTLQAFLNLSSEVRPALALSERALPKL
                     GPRVSNLETRQRAMRLISEGDTLFAAQNFNSALQKYKLAANVAPDVDEAYWRQGHAFV
                     ATKNFELAATAFKRAIANTDNLGRGGFRLDDLYGGASITKASHLESLAETALARNGDA
                     DAYFLIGLFLNYDGQAERASKFFARASELAGISGGHIAVFLDHEPAPVVRAAPAPATP
                     PAPQPQLFINSGAEI"
     gene            complement(4659..5387)
                     /locus_tag="VFB80_22420"
     CDS             complement(4659..5387)
                     /locus_tag="VFB80_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36609.1"
                     /translation="MWCSTCQQDVPALGSPSGGKPRCGKCSQPLAESAASPLDSAALE
                     RLLSDVPQGEDWALEAELRGMQRTLGAPRSPAPAAVHAPHPLAPAWHAPPAAPQPALE
                     PIETPKSPSHLAAWTILSLGLAVFACGAVLLGWSIAARREDLWPIGLPLALVGQAGLI
                     LGLILQLDGLWHTSRRTAKALTQLDGELASVRQATTLLSTSHASGAQSFYFHLAEGAS
                     PQLLLADLKGQLDLLAQQMATRKS"
     gene            complement(5547..6467)
                     /locus_tag="VFB80_22425"
     CDS             complement(5547..6467)
                     /locus_tag="VFB80_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZN36610.1"
                     /translation="MTATPAKPTPGLKDLLSSAQLPALPQSAIRLLELSQNPDNGPAE
                     FAVPIESDPGLTGQVLKFVNSSYFGFSREISSVKLAITLVGIRTIKNFSLWSAVFSLM
                     PNPKCGPFDLKSLWQDSLRRALFARACGKLLGLKEAEDLFAGALLQDMAIPLLAKEQP
                     ARYLKLLEGRHDGERRLSDLERVEFGWTHAEAAGFMARQWSLPEEFAQLIEAHTRLDD
                     FLTDGCKDPGKLAVALSALLPANNDDQWFEKDKFLAAYQQLVGPRGKPAEELFAQIDR
                     EFTEFAPVMKLATPAKTLTVHLVKAAIASV"
     gene            6919..7260
                     /locus_tag="VFB80_22430"
     CDS             6919..7260
                     /locus_tag="VFB80_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36611.1"
                     /translation="MSRVLTSLFAILLALAAVSQTVTAQTADDGWRRTANGWERVVLH
                     HDWVFSAAKSAEPAARFDTHPAALALFMVVTIIGAMVAFPQPKVCRLSWLNESWLDVF
                     RRSFRASVFGS"
     gene            7386..9599
                     /locus_tag="VFB80_22435"
     CDS             7386..9599
                     /locus_tag="VFB80_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36612.1"
                     /translation="MRLTLRTMLAYQDNVLEPADAEALSQKINESDFASGLSQRIRGV
                     MKKLRMDAPKLDGKGLGNDANTVAEYLDSALPQDRVGDFERVCLESDKHLCEVAACHQ
                     ILTLVLGKPADVSPELRERVYDLSDPERQAARSPGVGVTPPPVQAAVAANGKPADSEV
                     PDYLRAGRRAGVLPFLATIAATFLIAAFVLRMMGPFNASHPVLQWMRGGPIVAANTAT
                     NTQPTPDQTPPESVPAPPGPEMATPPAADSTKTPASPPATPATTPDSATPPAAVAADS
                     GDKPPPAPLPASPPETPAATPIAPAPAAPMPEAVVGRRPLVPAVPAVEPPPMDVGRYT
                     SDGQVLASFDPADGLWKLKPSQGALVAGERVIALPTYRPQLTLPGLQVTFASEGAVQL
                     AAPSEGNIPRLIVDQGKMLIVAVGAMNSQFDLDLGGVPLGTAGIRGLVTLVDPDSTVA
                     VKVMRWLPPGTDPAEGPGATVVELFNTHGRVTWQEQGQPKVEIPTHQVLLYAGASPPE
                     LQGPFIAPEWIDAKSVPPIDRQASLELDGMLADDRALSLALQEALQFRKVEVRSLAAR
                     CLTCLSEYEPALKEFSDERQKSYWIAEVEGLQRALHHSPEAAAGVKAAVERLRPAEAK
                     DLYRLLWGYSQEQLERGGAAQLVKFLEHEQMDVRVLAYANLLAITGAGELYRPERLPA
                     QQRAAIQNWKERQAKGTIAYKLPPTPLENYKPLANPAATEIPLPGRGVPAAPLAP"
     gene            complement(9678..10040)
                     /locus_tag="VFB80_22440"
     CDS             complement(9678..10040)
                     /locus_tag="VFB80_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36613.1"
                     /translation="MLRRLAAACLVLIIVGCGSPPDNTRSLGGSFAQIEGAVGDMHAN
                     VFGGRYSGDLGNDQLGFAMVEFQKSVKGTALEADGERLARKITEVTTLGSQRPPIAKL
                     RTAVKELEDMVADIKKKL"
     gene            complement(10092..11159)
                     /locus_tag="VFB80_22445"
     CDS             complement(10092..11159)
                     /locus_tag="VFB80_22445"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36614.1"
                     /translation="MNGKARGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRMQCA
                     NNLKQIGLGLHNYHSTHNLFPPGRMYPYFSPTIPCWTGAVSVHTHLMPYYELGNAYNR
                     FDFNFPRVRVPPLGAPFCPQNEAIVATHFPLFACPSEARRPNGVVTNNYRYNFGVTFC
                     VGVPWDDTGAEREPWSSNCRGELHGLAGGLFGDLVRGARDVTDGLSNTAAFSERVIGD
                     LDNAGLISPGDFRRTVPQNEQQTTATILASCTANLPLTGSHESFISVGPGAWTYGHPN
                     KTIYNHLFTPNSPIRDCSTNVSAVDGNNEGAIATARSYHPAGVNLLMADGSVRKVSNN
                     IELTVWRSVGTRSGGESLGDF"
     gene            11357..12061
                     /locus_tag="VFB80_22450"
     CDS             11357..12061
                     /locus_tag="VFB80_22450"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN36615.1"
                     /translation="MGRQQTKPRRPDGATRSQRKLGLATTRVYETLRERILAGELSPG
                     TRLSHRAIAEAMGTSNGPVVAALRRLAHDGLITNEPSQGGMVRQFNEDDLADAMILRR
                     ALETEAARLAARRASPEDVERLYAIITHMGDIVRREAWEEADEADIELHVAIARITRS
                     TGLMDALERCHLRDLVRRRLLASDRRRDFQNLERNHRNLVDAIASHDPDIAGQAMHQH
                     LTLKTPASRPCNSSVS"
BASE COUNT         2092 a         4000 c         4105 g         1973 t
ORIGIN      
        1 ggagaggagc cagtagcagg cacactccgt gtgccgtcgc ccgccgacga tcatccaccc
       61 gcaagtgacg gctcacggag tgagcctgct acgcataacc tgcataacgt gcataaagcc
      121 aacggccatg catcggctgc aagtgctgag ccggcctatg cttacgccga aaccggcagt
      181 ggagaaaaaa gtgcgaatgc aacgcatagc cagccagttc gcgcccgacg cagcaacggt
      241 catagtaacc gccggtcgaa ccgcgcctga agtcaggagc cgagcgtgag ccgcaagaat
      301 cgccgcaagc cgccgccgcg cgaaatcaaa gtccatcgcc ggacggagcc cgggtcgtcg
      361 ccgggccagg tgctggccga tccggcgcag ccgcagccgg tgatgcatgt gatcgcctac
      421 ggggccagcg gcgtggtgga ggctgaggtc aagaccgccg acgaagcaca cgccctggtt
      481 ggcaagcagc cgatgacctg gatcaacgtc gaaggcctgg gcgacgccga aaccatcgag
      541 cggttcgggg agctatttgg cctgcatcgc ctggccctgg aagacacggt gaacgtgcac
      601 cagcgggcga aagtcgagga ctacggcgag gtgctgttca ttgtgctgcg gatggtgcat
      661 tgcgggaacc tggccgacag ccgctgcggc accgagcaga tcagtctgtt cttggggccg
      721 aattggctgc tgacgtttca ggaagggcat ccgggcgaca gcttcgaccg cgtgcgggcc
      781 cgcatccgcg agaaaggcgg ccggatgcgc acgctcggcg gcgattacct ggcctacgcc
      841 ctgatcgacg ccacgatcga taactactat cccgtgctgg aagtttatgc cgagcggctg
      901 gacgagctgg aagagcaggt gctcaatccc cgcagcgtac gggccgtcga tcatctgcac
      961 gaggtgaagg ccgacctgct ggttctgcgg cgggccatct ggccgctccg cgacgcgatg
     1021 gcctacctgt cccgcgagga gacggcgcga ttcacggaag cgacgcggcc gtatctccgc
     1081 gactgctacg accacatcgt gcaggtcgtc gagctggtcg agacctaccg cgagctgacc
     1141 gccgacctcc gcgacctcta catgtcgtcg ctgagcaacc ggatcaacga aacgatgcgg
     1201 gtgctgacga tcatctcgac actgttcatc ccgctgacgt tcatcgccgg catttatggc
     1261 atgaacttcg acccggatac gtcgccgtgg aacatgcccg agctgcgctg gtacctcggc
     1321 tacccgctgg cgctggtcgc gatggtcgcg atcggcgtgg gaatgctcat ttactttgtc
     1381 cgccagggat ggctgttccg cgggaagggg taggtgcgtg ctggcgacaa gttgtcacga
     1441 gccggtcagt cggtcggtcg gtacatcggt ccgtcggtat ggcggccacc gacctactga
     1501 cttaccgacc taccgaccaa ccggccttgg gggcacgcga tatgctcccc ccggcgatca
     1561 gggagcgccg tccgcacgcg ttgcagccgg tgctaggggg aattcgccaa aaacacgagc
     1621 tttcaggaga tggaaatgaa tgcgatttat tggaatcgaa ttggccgctg ggctgccggc
     1681 ctgaccgccg gcgcggcgct ggccatcgcc ggccccgctt ggagccagca gccgccgaca
     1741 ccggccccag cgccgaaagc gccggtgacg ccgaagcaag atcccgtcaa gccggctgcc
     1801 gatccagcgc gcgaggcggc ccgcgatcgg gtcgagggcg cgcgcgaagc ggctcgcgac
     1861 cgcgtcgagg gggcccgcga gggtgcgcaa gacgcccgcc gcgacgcccg cgaaggagct
     1921 caagacgctc gccgcgatat ccgcgacgcg cggggcgatc tccgcgaagc ccgccgcgac
     1981 gcccggcaag ctctgcgggc cgcggacctt ggactatggt tcggcgctcg ggccggtacg
     2041 accggcctgg ttgtcgccga cgtcgccacg cagggcgcca ttgcccgcgc cggcttcctg
     2101 gaaggcgacc gcctcatctc cgtcaacggc actcccgtca ccacggaggc cgagttcgtc
     2161 cgcctattca ccgacgacgc catccgcgat cagcaggtca aggtcgtcgt gatgcgcggc
     2221 ggcaaagagc aggtcatcgt ggtgcagccg tcggtgctca tccaggaggc tgtggcctac
     2281 gacccgctct ggcagtacgg gatcatcctc gacgaccgga cgcctgaccg cctggtgatt
     2341 cagcgagtgt tcccgcggac gccggccttc tatgccgggc tgcgttcggg cgacgtgatc
     2401 gtttcggctc gcgggcagcg gctggctgcg gcggccgact tgctgcgggc actctcggcc
     2461 aacaccgatc ggctcgcgct gcagatcaac cgcgccaacg cgatgcgcga cgtgcaactg
     2521 gagtcgagct ccaacttcgg cgccgatgtg cagacgctgc tgcggccgcg gatcgacgat
     2581 ccgaaccgcc cggcgactcc ggccgacacg cagccgcggc tggatgcgcc gcgcccgggc
     2641 acgccagcgg ttccaccgcg gcccggcacg acgccggcga cccccgcggt gcccggccgt
     2701 ccggcgactc cgccgacaac cacgacgccg gcgctgccgg ccgttccgcc gatcccggcg
     2761 acgccgccgg ctgcaccagc gcccaccgcg cctgcggcgc ccgctgcgcc ggcgcccgcg
     2821 ccggccgcgc cgagcgcacc gtaagaaagc gcacgacgaa atcaaggatg tccgccgcgg
     2881 cctcagcctc gcgctggggc tgcggccatt tcttgcgtgt acaatgccag gtgtgaacct
     2941 ggttcgggat attccctgcc tgtgaaatca cgatcgaccg atcagcgcct gattcgcccg
     3001 ccgcagttcg gcttgcggac gctgctgctc gttgtcaccg cgtgcagcgt cttgtttgcg
     3061 ctgggtcact ggctgtcgcc gatcgccttt gtcggactgc tcctgctcgt cgcgtcgatt
     3121 acgctgcacg tcgccggcaa cgcgatcggc acgcggctgc gcgagctcgg tgaccgcccg
     3181 ccgcctgacg acgccgcggc caaccgtgcg ggagagccac gtccgctgga attcgcgtcg
     3241 gcgacgcgtc tgagcgagcg gcagggactg ggctgggtga tttacgcagc gacaattgcc
     3301 ggcacgctgg ccggcggaat cggcggcggc gtttggacct ggctgaccag ccgcgagccc
     3361 gcgggcctct tgcaactcgc cgtcggcgtg atggcgttcg cggtgctggg cggcctggcg
     3421 gcgttcgccg cggcggcgtt cacgcaggtc ttgctggggg cgttgtggca agcgctgcac
     3481 ccgctgcccg cggctcgtcc gacttcgctt gcttctgccg acggagcgcg cgataatccc
     3541 gacctggggc attccaattg acctggaagg agttgggcca tgcgtcgctt aatcctgatc
     3601 ctcgccgcgt ttatcggact ctcgctcgtc gctgcgccgg cacaggcctg gtgggggtgc
     3661 cactggcgcc actgcggctg gggctatcgc gggtgcgggt ggggcggata ctgcggctat
     3721 cgcggttatg gctggggcgg ctatggcaat tactgcggct tcggtccggc ttattactac
     3781 ccggcgtaca actatccgct gatcaactac ggtggctacg gctacggctg cggctacggc
     3841 tacggcgggc tgaattatgg cacgccgatc agcatcggct attacgccaa ccgcggcggc
     3901 tactacaacg cgaactcgaa tttcgccctc gccgcgctgc cgcagccgcg aacaccgatt
     3961 ctgaccaacg tcgatgccca gacgctgcaa gcgttcctga atctcagcag cgaggtgcgg
     4021 ccggcgctag cgctgtccga gcgggccctg ccgaagctcg gcccgcgggt ctcgaatctc
     4081 gagacgcggc agcgggcgat gcggttgatt tccgaagggg acacgttgtt tgcggcgcag
     4141 aacttcaact cggcgctgca gaagtacaag ctggcggcca atgtcgcgcc ggacgtggac
     4201 gaagcctatt ggcggcaagg ccacgcgttt gtcgccacga agaacttcga actggcggcg
     4261 accgcgttca agcgggccat cgccaacacc gacaacctgg gccgcggcgg attccggctg
     4321 gacgacctgt atggcggcgc gtcgatcacc aaggcgtcgc acctggagag tttggccgag
     4381 acggcgctgg cccgcaacgg cgacgcggac gcgtatttcc tgattgggct gtttttgaac
     4441 tacgacggac aagccgagcg ggcgagcaag ttcttcgcgc gggcttccga gctggcgggc
     4501 atcagcggcg ggcacatcgc ggtgttcctc gaccacgagc cagcgccggt ggtacgggcc
     4561 gcgcctgcac ccgctactcc gcccgccccg cagccgcagc tgttcatcaa cagcggcgcg
     4621 gagatttaat agtgacggct cgcggaacga gcgggctact atgatttccg cgtcgccatc
     4681 tgctgagcca gcaggtcaag ctggcccttc agatcggcga gcaacagctg cgggctggcg
     4741 ccttcggcca gatggaaata gaacgactgc gcgccagagg cgtgcgaagt cgaaaggagc
     4801 gttgtcgctt gccggacgct ggccaattcg ccgtccagct gcgtgagggc cttcgcagtg
     4861 cggcggctcg tgtgccacag tccgtccagc tgcaggatga ggccgagaat caggccggct
     4921 tgcccgacca gggccagcgg caggccgatc ggccagaggt cttcgcggcg ggccgctatc
     4981 gaccagccga gcagcaccgc gccgcaagcg aagacggcca gccccagcga gaggatcgtc
     5041 caggcggcca ggtgcgaggg cgacttgggc gtttcgatcg gttcgagagc cggctgcgga
     5101 gcggcgggcg gcgcatgcca ggcgggcgcc agcggatgcg gggcgtggac tgctgccggc
     5161 gcggggctgc ggggcgcgcc gagcgttcgc tgcatgccgc gcagctcggc ttccagggcc
     5221 cagtcctcgc cctgcggcac gtcgctgagc aagcgctcca gggcggcgct atcgagcggc
     5281 gaggccgcgc tttcggccaa cggctgcgag cacttcccac agcgcggttt gccgccgctg
     5341 ggcgagccca gagccgggac atcctgctgg caagtcgagc accacataca ccaggccgca
     5401 tcacaattgc cagggccgcg caagcgcgga cgccgctagc acggcccgaa gatggaccat
     5461 gccttaccat cggaatcggc gcgaggcgcg cgaaactata ccgattctgc cggttttgcg
     5521 ggcagccgaa ggatagctgg ccaacgctac acgctggcga tggccgcctt gaccaggtga
     5581 acagtaagcg ttttggcggg cgtggccagt ttcatcaccg gggcgaactc agtgaattcc
     5641 cggtcgatct gagcgaacag ctcttccgcg ggcttgccgc ggggaccgac gagctgctgg
     5701 taagcggcga ggaatttgtc cttctcgaac cactggtcgt cgttgttcgc cggcaagagc
     5761 gccgagaggg ccacggccag cttgcccggg tctttgcagc cgtcggtgag gaagtcgtcg
     5821 agccgggtgt gggcctcgat cagctgggcg aattcctcgg gcaggctcca ctgccgggcc
     5881 atgaagccgg cggcttcggc gtgggtccag ccgaactcga cccgctccag gtcggagagg
     5941 cgcctttcgc catcatggcg gccttcgagc agtttgaggt agcgggccgg ctgctccttg
     6001 gccaagagcg ggatcgccat gtcttgcagc agagcgccgg cgaagagatc ttccgcctct
     6061 ttgaggccca gcagcttgcc gcaggcccgc gcgaagaggg cccggcggag cgagtcctgc
     6121 cacaggctct tcaggtcgaa cggcccgcac ttgggattgg gcatcaggct gaagaccgcg
     6181 ctccacagcg agaagttctt gatcgtgcgg atacccacga gcgtgattgc cagcttgacg
     6241 ctcgagatct cgcgcgagaa gccgaaatac gacgagttga cgaacttcag cacctgcccc
     6301 gtcaggcccg gatcggactc gatcggcacg gcgaattcgg ccgggccgtt gtcggggttc
     6361 tgcgacagct cgagcaggcg gatcgcgctt tgcggcagcg ccggcagctg agccgagctg
     6421 agcaggtcct tgagtccggg agtgggtttc gcaggggtcg cggtcataaa cggacgctcc
     6481 aaggggtcca gccggcctcg cgcaaaggcg gagccggcgc gcggtgccgg gggaatgtgc
     6541 ctgacacgga aacctaggac gcggtcgatt gtccggcaaa tcgacgtgcg caaggccgct
     6601 cgcgcaggct gcccggccgt cggattgtgc cggtcgcagc ggctgcgcca tttccacccg
     6661 catgtggcaa aagctcgccg cgctgcggcc aggcaacgtg catagcgccg cgcgttgcgg
     6721 gagagcaaca cgccgcgcac gggcaaaatt tggcgagtat gcctaaaccg ttgattccca
     6781 agcgcttgag gctcagccgc agaggatccg cagcgagggc actggcattg cgaattgggt
     6841 ctgtcgccgg cacgagcccg cagcacccat ttcgatgcag aaagggcgcg ggactggccg
     6901 gcggcaaggc caccattcat gtcgcgcgtg ctgacctctc tatttgcgat cctgctggca
     6961 ctggccgctg tcagccagac agtgacggcc cagactgccg acgacggctg gcggcgcacg
     7021 gcaaacggct gggagcgcgt cgttctgcac cacgactggg tcttctccgc ggcgaagtcg
     7081 gccgagccgg ccgctcgctt cgacacgcat ccggcggcgc tggcgctgtt catggtcgtc
     7141 acgattatcg gtgcgatggt cgccttcccg cagccgaagg tctgccgcct ctcctggctg
     7201 aacgaaagct ggctcgacgt ctttcggcgc tcttttcggg cttcggtatt cgggtcgtaa
     7261 gtcacggccg gggcacgctt tatcttccgc gccgtcagaa ttccccgccg gcagttgcac
     7321 ggattggccc gccgcttcgc actattacct gtagagcgaa atgcgactcg aggcggagag
     7381 ctgccatgcg attgacgctg cggacgatgc tggcctatca ggacaacgtg ctggagccgg
     7441 ccgacgccga agctctcagc cagaagatca acgaaagcga tttcgcgtcg gggctgtcgc
     7501 agcggattcg gggcgtaatg aagaagctgc gaatggacgc gccgaagctc gacggcaagg
     7561 gcttgggcaa cgacgcgaac acggtcgccg agtatctcga tagcgctttg ccgcaggacc
     7621 gtgtcgggga cttcgagcgg gtctgcctgg aatcggacaa gcacctctgc gaagtcgcgg
     7681 cctgccatca gatcctcacg ctggtgctgg gcaagccggc ggatgtctcg ccggagctcc
     7741 gcgagcgcgt gtacgacttg agcgatcccg agcgccaggc ggcccgctcg ccgggcgtcg
     7801 gcgtgacccc gccgccggtg caagcagccg tggcggccaa cggtaagccg gccgacagcg
     7861 aggtgcccga ttatctgcgg gcaggccgcc gcgcgggggt cttgccattc ctcgcgacca
     7921 ttgcggccac gttcctcatc gcggcgttcg tgctgcgaat gatgggtccg ttcaatgcat
     7981 cgcatccggt cctccagtgg atgcggggcg gcccgatcgt cgcagccaat accgccacaa
     8041 acacccagcc aacgcccgac cagacgccgc ctgagtcggt tcccgcgccg ccaggcccgg
     8101 aaatggccac accgcctgcc gccgattcga caaagacgcc cgccagcccg ccggctacgc
     8161 ccgcgaccac gcccgacagt gcgactccgc ccgccgctgt cgctgccgat tccggcgaca
     8221 agccgccgcc cgcgccgctg ccggcctcac ccccggaaac gcccgcggcc acgcccatag
     8281 cgccggcgcc cgccgcgcca atgcccgagg ccgtggttgg ccgccggccg ctagtgccgg
     8341 ctgtgccggc cgttgagccg ccgccgatgg acgtcggccg ctatacctcc gacggccagg
     8401 tgctggccag cttcgatccg gcagatggac tttggaagct gaagccgtcg cagggcgcac
     8461 tcgtcgccgg cgagcgggtg atcgcgctgc cgacctatcg cccgcagctc acgctgccgg
     8521 gattgcaggt gacttttgcc agcgaaggcg cggtgcaatt ggcggcgccc tccgaaggca
     8581 acatcccgcg gctgatcgtc gatcaaggca agatgctgat cgtcgcggtg ggcgcgatga
     8641 actcacaatt cgatctcgac ctgggcggcg tgccgctggg aacggctggc attcgcggcc
     8701 tggttacgct ggttgatccc gattcgaccg tggcagtcaa agtgatgcgc tggctgccgc
     8761 cggggaccga tcccgccgaa gggcccggtg cgacggtggt cgagctgttc aacacgcacg
     8821 gccgcgtcac ctggcaggag caaggccagc ccaaggttga gattcccact catcaggttc
     8881 tgctgtacgc cggcgccagc ccgcccgaat tgcaggggcc gttcatcgcg cccgagtgga
     8941 tcgacgccaa gagcgtcccg ccgatcgacc ggcaggcctc gctcgagctg gatggcatgc
     9001 tagccgacga tcgggcgctg agcctggcgc ttcaggaggc gctacagttt cgcaaggtgg
     9061 aagtccgctc gctggccgcc cgttgcttga cttgcctgag cgagtacgag ccggccctca
     9121 aggaattcag cgacgagcgg cagaagtcgt attggatcgc cgaggtggaa ggattgcagc
     9181 gggccctgca ccattcgccc gaagcggccg ccggcgtgaa ggccgcggtc gaacgcctgc
     9241 ggccggccga ggccaaggac ctgtaccgct tgctgtgggg ctacagccag gagcagctcg
     9301 aacggggcgg ggcggcgcag ctcgtcaagt tcctggagca cgagcagatg gatgtgcggg
     9361 tgctggccta tgccaacctg ctggcgatta ccggcgcggg cgagttgtat cggcccgagc
     9421 gcctgcctgc ccagcagcgg gccgcgatcc agaactggaa ggagcggcag gccaagggca
     9481 ccatcgccta caagctgccg cccacgccgc tggagaacta caagccgctg gctaatccgg
     9541 cggcgaccga gattccgctt ccaggccgcg gcgtccccgc ggcgccgttg gcgccttaag
     9601 ggtccaaaca agctgcaatc tcaaaactcg ggatcccgtt tttgcgaatt cctgcccgtt
     9661 tccagcgggt cgccagatca gagctttttc ttaatgtcgg ccaccatgtc ctccagctcc
     9721 ttcaccgccg tgcggagttt ggcgatcggc ggacgctggc tgcccaacgt ggtgacttcg
     9781 gtgattttcc gggccagcct ttcgccatcc gcctccaagg cagttccctt gactgatttc
     9841 tggaactcga ccatcgcgaa tcccaattga tcattgccca ggtccccgga ataccggccg
     9901 ccgaagacat tcgcgtgcat gtcccccacg gctccttcga tttgtgcaaa cgatccgccc
     9961 agggacctcg tgttatccgg cgggctgccg catcccacga taatcaggac gaggcacgcg
    10021 gcggcaaggc gtcgaagcat ggcagttctc cgcgagtgtt gccgagttgc cgtcacagag
    10081 aaatgaagcg gctagaagtc acccagcgat tcgccgcctg accgcgtgcc gacggaccgc
    10141 cagacagtca actcgatgtt gttgctgacc ttgcgaaccg aaccatcggc catgagcagg
    10201 ttcacgccag cgggatgata actgcgggcg gtcgcgatcg cgccctcgtt gttgccatcc
    10261 acggcggaga cattggtgct gcaatcgcga attggcgaat tgggtgtaaa caggtgattg
    10321 taaatcgttt tgttcggatg accgtaggtc cacgctccgg gtcctaccga gataaagctc
    10381 tcgtgtgaac cagtcagagg gagattggcc gtgcaggaag ccaggatcgt cgccgtggtc
    10441 tgctgctcat tctgcggaac ggtccgccgg aaatctcccg ggctgatcag cccggcattg
    10501 tccaggtccc cgatcacgcg ctcactgaac gccgccgtgt tcgaaagccc gtcggtcacg
    10561 tcgcgagccc cccgcacgag gtcgccgaac aggcctcccg ccagtccgtg caattcgccg
    10621 cggcaattgg aactccaggg ctcccgctct gctccggtgt cgtcccaggg cacgcccaca
    10681 cagaacgtca cgccgaagtt gtagcgataa ttatttgtca caacgccatt gggccggcgg
    10741 gcttccgagg ggcaggcgaa gagcggaaag tgcgttgcga caattgcctc gttctgcgga
    10801 cagaaagggg ctcccagcgg cggcacgcgg acccgaggaa aattgaagtc gaagcggttg
    10861 taggcgtttc ccagctcata gtacggcatg agatgtgtgt gcaccgagac ggcgccggtc
    10921 caacacggaa tcgtcgggct gaaatagggg tacatccgac ccggcggaaa caggttgtgg
    10981 gtgctgtggt agttgtgcag gccgagcccg atctgcttca ggttgttggc gcactgcatc
    11041 cgccgcgcgg cctcccgcgc cgcttgcacg gctggcaaga gcagggcgac caggacgccg
    11101 atgatcgcga taacgacgag caattcgacc agcgtaaagc cgcgcgcttt gccgttcatc
    11161 tttgcgctcc gcacccagaa agtggaatcg cagggagcca gactcaccgt gccgagcggc
    11221 gcctggggcc gaaatcgcca agccactcgg acggcaacct aggcacgtga gtaattgtga
    11281 gtactcacgc gggattgtac aatcttcatg gcccggtttg caagcggaca gccattcacg
    11341 cgaggcaggt acagtcatgg gcaggcagca gacgaagcca aggcgaccag acggcgcaac
    11401 gcgcagccag cgcaagctcg gactggccac gacaagagtc tatgaaacgc ttcgcgagag
    11461 aatcctggca ggcgagcttt cgccgggaac gcgcctcagc caccgggcga tcgcagaggc
    11521 catgggcacc agtaacggtc ccgtggtcgc cgcgctccgc cggctggctc acgacggact
    11581 gatcaccaac gagccctctc agggcgggat ggtcaggcaa ttcaacgaag acgatctggc
    11641 cgatgcgatg attcttcgcc gcgcgctgga gaccgaagcg gcgcgcctgg cagcgcggcg
    11701 ggcctcgccg gaggacgtcg agcggctgta cgccatcatc acccacatgg gcgatatcgt
    11761 gcggcgcgaa gcgtgggagg aggcagacga ggcggacatc gagctgcacg tcgcgatcgc
    11821 gcggataacc cggagcacgg gactaatgga cgccctggag cggtgccacc tgcgcgacct
    11881 tgtgcggcga cgcctcttag ccagcgatcg ccgccgggac tttcaaaacc tcgaacgcaa
    11941 tcatcgcaac ctggtcgatg ccatcgcctc gcacgatccg gatattgccg gccaggcgat
    12001 gcatcagcac ctgacgctta agacgcctgc cagccgccca tgtaactcat cagtatcttg
    12061 atcggcgtga aggccgcggt cgaacgcctg cggccggccg aggccaagga cctgtaccgc
    12121 ttgctgtggg gctactgcca ggagcagctc gaacggggcg gcgcggcgca
//
LOCUS       DASRJB010000854         6914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3067327, whole genome shotgun sequence.
ACCESSION   DASRJB010000854 DASRJB010000000
VERSION     DASRJB010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067327"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(551..3973)
                     /locus_tag="VFB80_22455"
     CDS             complement(551..3973)
                     /locus_tag="VFB80_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36616.1"
                     /translation="MAIAFQCHKCGGKFAAPDHLAGQQARCQRCGTVVNVPLASAVPV
                     SPLRQPQPSLVSNVPWTAILAGGGLLLFLGFGALCLFVFVQIVWPKLQTLADRTTVPA
                     VSPQPPVTPQPTPNVPTAKVPTSTPNPFETTPSSASPTPATPNSSAPGSGRPSGSGDA
                     PGLPNLPTSSGPRPAAGNNSAPALPPTSWSARPDPPLAPVEYKKGKIAIPLPSNAELR
                     LPYGPSHFILGFARDFQKEIWEVIDLRTGKGIGKPIQAKLDIDYRSEAFSPDGRYLAA
                     LQRRSGDEIPIGVWSFISGQMERVLRVKGWRVSAPLRFGAAHQLVSLHEQEHNLSVLT
                     LWNLQTGDKVREIGFPAGSGENRLQPESLAVSPGGKYATLVMGNQLGVFDLTTGQPAG
                     NAVLPVVPSSCEGTAFSPDGRELALVVGSGSGHRLFIVDFSSGRIDVDQDYRGNLRFF
                     FYDGPVLDWLPDKSGLIWRGHILLERTSGEEVWYFDTTDGNPRRFIRDNELLVLVRDR
                     GQKLLRTFDLPEKEIDKAMLAVREGGVAIDAALPPLTTPDTFGVTQQTLPNGRTAWSL
                     SPDAAGPTPRGQERELLVAKSGENVRGVMFAGTSAGKVVVQKEITPPAGPGFRPARQV
                     VIERYDLTSGERGQSLEVPHVYQLADVSPSGRFAVVAFAPEANKYDRLDILGLAPKKH
                     VAAWRPYGGEKEQPAAAGNNQQARFPSLWGLPSDPRSIAWISMLDDEHLLTVNTTGKL
                     VCWKLPECKALYAFADFGEPLAVSAGRKYLAGGHSGEFRVFEALTGKCVGDLDSPLSG
                     SRAIRGAFRPDGQELVAVIDAGTDKMLVRWDMKNGKLTQEVPIPAVAVSSYVPFYAAH
                     NGVRLGMEYRGSNFLMIDDQFLVDLSKRALVWRYHLPQGHFAANSPDDRTWYCIRKNN
                     VADQSMFLTSIDTPSNSVLNKSQGKSLEKQLVLYPGMSVRVAVDLSAAGMSDLTPTVE
                     KAVAQGLEARGLVIDPAAPLVFSVVAAQRATGEQIQVYSGSSPFGYNPFFNPGQPSQT
                     ISQEELAVRLSVSDAGGKVLWHMDRSARMRAFGSVRSGDAGTQLRDEMVKQFESVLAG
                     SGSATQSLPRYIFGDLDSILAGESQLGFHDESPPPQSQLPAARQPPGIAPGS"
     gene            4482..5210
                     /locus_tag="VFB80_22460"
     CDS             4482..5210
                     /locus_tag="VFB80_22460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZN36617.1"
                     /translation="MAKRIIREYQRGLLYDQGRFVEVLPPGQYRIWPWQSKQIEIVDI
                     RETSQTVEGQEILTSDKIGVRVTLIAQFRVVDPVAARHQVENFVAQLYQDLQLTLREA
                     VTGQTLEALMSSREALSAGVQAQVAPRASKYGVELSRVGVKDIVLPGQVRNVFLQEME
                     AELKGRASLVAARHEIAAARARANTAKLFQENPHLLRMQELELLNNLASKSGNVIVIP
                     GLDRLLTREPSAAEPSTQPPASKA"
     gene            5207..6073
                     /locus_tag="VFB80_22465"
     CDS             5207..6073
                     /locus_tag="VFB80_22465"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HZN36618.1"
                     /translation="MIELLSRLLGRGSRADAGLQRLAAILSVPAAELAAVPGNPRLCY
                     RQFEIRKRSGRIRQIAAPSGALKLHQRRLLKQYLSQQYVHAAATAFRSGSSIATHARR
                     HLGQQIVLTVDLADFFPATAARRVRRWFREQGWEGAALDVLMRLTVYRGGLPQGAPTS
                     PALSNLVNRSLDEELSELAGQSGGRYSRYCDDLAFSFRTEAEPMTLRPQVEACLARHG
                     YEVQAEKGWRLQAAAEHPEITGLVLAGRRLRLADHILRRIQALRTRWFGLSANDRQRL
                     AGYRGLAKMIKR"
     gene            complement(6083..6607)
                     /locus_tag="VFB80_22470"
     CDS             complement(6083..6607)
                     /locus_tag="VFB80_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36619.1"
                     /translation="MSQVLERGAPPSSPAKQPAVPLATILSGVALAISCGVACWTFSH
                     DPLGRGLAAYDLSSPKAALVSQLKMLESQDYRAILESADQFNAKRAQDKLRTLEFRKE
                     SEFRGKKILFISYEENGIRKHDIQSFEKDAETGAWKSAYASITSVFEGSDETPDEKKL
                     RGMIKKWRDSGALE"
BASE COUNT         1149 a         2333 c         2290 g         1142 t
ORIGIN      
        1 gtgtttcaaa tcggaaaatg ccccggccct cttttgggga gcccggactg cgcggaccac
       61 aacctattgt ggtttggcga aaggccacca caatctgttg tggttgactt gacagatagt
      121 ggactgaaag ttacaattcc aaaccgcggc cggctcgcgg cggccagcac tgtccttggc
      181 aatttggtcg tccaggtcgc ccggccacag gcaaacgcgc cagtttccgc ggcattcgga
      241 gtttttcacc acggagcctc gcctcgccac ccggtgaaga acgccggagt cccgaagcag
      301 atgaggaggc gcacggccga caggaggcga attgttcgcc acaccaattc gctcgtaact
      361 gctgatcccg caaggcgttt gcgatcggcg gcggtgaaat tatcgccaga tcctcaggta
      421 tcgagggcaa ttggcgaata aatcgcggcc aaaactactg caaagcctgg gaatttcgcg
      481 aatcgcggcc gaattattcg ccaccgagct gtccgacccg gcgaagaact cgtcggcccg
      541 agacgttgca ctaggagcca ggagcaatcc ccggcggctg ccgcgcggcc ggcagctgcg
      601 actggggcgg cggcgattcg tcgtggaagc ccagctgtga ctcgccggcg aggatcgagt
      661 ccaggtcgcc gaagatgtat cgaggcagcg actgcgtggc cgagccgctg ccggccagga
      721 cgctctcgaa ttgcttgacc atctcgtcgc ggagctgagt tcccgcgtcg ccgctgcgga
      781 cgctgccgaa ggcccgcatc cgggcgctgc ggtccatgtg ccacagcacc ttgccgccgg
      841 cgtcgctgac cgacagccgc acggccagct cttcctgcga gatcgtctgg gagggctggc
      901 ccggattgaa aaacgggttg tagccaaagg gcgagctgcc gctgtaaacc tggatttgct
      961 cgcccgtcgc tcgctgggcg gcgacgacgg agaacacgag gggcgcggcg ggatcgatga
     1021 ccaggccgcg ggcctccagg ccctgggcga cggccttctc gacggtcggc gtcaggtcgc
     1081 tcatgcccgc cgccgacaga tcgaccgcga cccgcacgct cattcccggg tagagcacga
     1141 gctgcttctc gagcgacttg ccctgcgact tgttgagcac actgttagag ggcgtgtcga
     1201 tcgacgtgag gaacatcgac tgatccgcga cgttgttttt gcggatgcaa taccaggtgc
     1261 ggtcgtcggg cgaattcgcg gcaaagtgcc cctgcggcag gtggtaccgc cagacgaggg
     1321 cccgcttgct gagatcgacg aggaactggt cgtcgatcat caggaagttg ctgccgcggt
     1381 attccattcc cagccgcacg ccgttatgcg ccgcgtaaaa cggcacatag ctgctgaccg
     1441 cgacggcggg gatcggcact tcctgggtga gcttgccatt cttcatgtcc cagcggacga
     1501 gcatcttgtc ggtgccggca tcgataacgg cgaccagctc ctggccgtcg gggcggaacg
     1561 cgccgcggat cgcccgcgag cccgagagcg gcgaatcgag gtcgccgacg cacttgccgg
     1621 tcaaggcctc gaacacgcgg aactcgcccg agtgcccgcc cgccaggtac ttgcggcccg
     1681 cgctcaccgc cagcggctcg ccgaaatcgg caaaggcata cagcgccttg cactccggca
     1741 gcttccagca gaccagcttg ccggtcgtgt tgaccgtgag caggtgctcg tcgtcgagca
     1801 tcgaaatcca ggcgatcgag cgcggatcgc tgggcaggcc ccacagcgac ggaaaccgtg
     1861 cttgctggtt attgcccgcg gccgccggct gctccttctc gcctccatac ggccgccagg
     1921 cggcgacgtg tttcttgggg gccagtccca ggatgtccag ccggtcgtac ttgttggctt
     1981 ccggcgcgaa ggcgacgacg gcgaaccgcc cgctggggct gacgtcggcc aactgataga
     2041 cgtgcggcac ctcgagcgac tggccgcgct cgccgctcgt gaggtcgtag cgctcgataa
     2101 cgacctgccg cgcggggcgg aaccccggcc cggccggcgg cgtgatttcc ttctgcacga
     2161 cgaccttgcc ggccgatgtg ccggcgaaca tcactccgcg gacgttctca ccgctcttgg
     2221 ccaccaagag ctcgcgctcc tgcccgcgtg gcgtcgggcc cgccgcgtcc ggcgacagcg
     2281 accaggccgt gcggccgttg ggcagcgtct gctgcgtcac gccgaacgtg tcgggcgtcg
     2341 tcagcggcgg cagggcggcg tcgatcgcca cgcccccttc gcggaccgcc agcatggcct
     2401 tgtcgatttc cttctccggc aggtcgaacg tgcggagcag cttctggccg cggtcgcgca
     2461 ccagcaccag caattcgttg tcgcggatga accgccgcgg gttgccgtcg gtcgtatcga
     2521 agtaccagac ttcctcgccg ctggtccgct ccagcaggat gtggccgcgc cagatcaggc
     2581 cgctcttgtc gggcagccag tcaagcaccg gcccgtcgta gaaaaagaac cgcaggttgc
     2641 cgcgatagtc ctgatcgacg tcgattcgcc ccgacgagaa gtcgacgatg aacagccggt
     2701 ggccggaccc ggagccgacc accagcgcca gttcccggcc gtcgggcgag aaggccgtcc
     2761 cttcgcacga cgagggaacg accggcagca ccgcgttccc cgccggctgg cccgtcgtca
     2821 ggtcaaaaac gccaagctga ttgcccatca ccagcgtcgc gtatttaccg cccgggctga
     2881 ccgccagcga ttccggctgc aggcggttct cgccgctgcc cgcgggaaaa ccgatctcgc
     2941 ggaccttgtc gcccgtttgc agattccaca gcgtcagcac cgagaggttg tgctcctgct
     3001 cgtgcagcga gacgagctgg tgcgcggccc cgaaccgcag cggggcactc acccgccagc
     3061 ccttcacccg cagcacccgc tccatctggc cggagataaa cgaccagacg ccgatcggga
     3121 tctcgtcgcc gctgcggcgc tgcaaggccg ccaggtatcg cccgtccggg ctgaaggcct
     3181 cgctgcgata gtcgatgtcg agcttggcct ggatcggctt gccgattccc ttgcccgtcc
     3241 gcaggtcgat cacctcccag atttctttct gaaagtcgcg ggcgaaaccc aggatgaaat
     3301 ggctcggccc atacggcagc cgcagctcgg cgttgctcgg cagggggatg gcaatcttgc
     3361 ctttcttgta ttcgacgggg gccagcggcg gatcggggcg agcggaccag ctggtcggcg
     3421 gcagagcggg cgctgagtta ttgccagccg cgggccgcgg gccggagctc gtgggcagat
     3481 tcggcagccc cggagcgtcg ccgcttccgg acggcctgcc ggagccaggc gctgacgagt
     3541 tgggagtcgc gggtgtgggc gacgcggacg acggtgtcgt ttcgaacggg ttaggggttg
     3601 atgtgggaac cttggccgtc ggcacattgg gcgtcggctg cggcgtgacc ggcggctgcg
     3661 gagacacggc gggaacagtc gtccgatcgg ccagggtctg cagctttggc cacacaattt
     3721 gcacgaacac gaacaggcac agcgcgccga atccgaggaa caagagcagc ccgccgcccg
     3781 ccaggatcgc cgtccagggc acgttgctga ccagcgaggg ctgcggctgg cgcagcgggc
     3841 tgacgggcac cgccgaagcc agcgggacgt tcaccaccgt gccgcaccgc tggcagcggg
     3901 cctgctggcc ggccaaatga tccggcgcgg caaatttgcc gccgcacttg tggcattgaa
     3961 acgcgatcgc cataacacgc tcaccacgta gccggcgctg ccagcgtcgg ccgccgtacg
     4021 tcttccctcc ggcggacgct ggcagcgtcc gctacgagcc agattgccaa cttagctgcc
     4081 cgtttccgcc gcggcaacgt gctggcgttt gcccttgcgg cggctatact cggccgcgag
     4141 tcagcgcggc gccggcctgg ttttggttcg gcgtaccgcc gtaagacccc caattgccag
     4201 ggccgcgcaa gcgcggcctt ggcatcgcca aggggacgat ccgatggcga gcttcggctc
     4261 gttgcggctg gtcgtcttgc ggggtggggg atgcctgacg cccatcagct gccggttcgc
     4321 cggcggccgg gcgtcatcaa cccgcctccc acgaccgggc gtattcggac ggccgcgggc
     4381 gcactttttg cgctcgctgc cgccaaaagt cctcttggat cgcgattgac cgccgcgtgc
     4441 cgcggcgtga aacataacgt gcttgagaac aggaggcagc cgtggcgaag cggattatcc
     4501 gggaatacca gcggggcctg ctctatgacc agggccggtt cgtcgaggtc ctgccgccgg
     4561 ggcagtatcg aatctggccc tggcagagca agcaaatcga gatcgtggac atccgcgaga
     4621 cgagccagac ggtcgaggga caggaaatcc tgaccagcga caagatcggc gtccgcgtga
     4681 ccttgatcgc gcaattccgg gtcgttgatc cggtcgccgc gcggcaccag gtcgagaact
     4741 ttgtcgccca gctgtatcag gacctgcagc tcacgctccg cgaagcggtg acggggcaga
     4801 cgctggaggc cctgatgagc agccgcgagg cgctctcggc gggcgtgcag gcgcaggtcg
     4861 cgccgcgggc ctcgaaatac ggcgtggagc tgtcgcgcgt cggcgtcaag gacatcgtgc
     4921 tgcccgggca ggtgcggaac gtcttcctcc aggaaatgga ggccgagctg aaggggcggg
     4981 ccagcctcgt cgccgcccgc cacgagatcg ccgccgcgcg ggcccgcgcc aatacggcta
     5041 agctcttcca agaaaatccg cacctcttgc ggatgcagga gctggagctg ctcaacaacc
     5101 tggcctcgaa gtcgggcaat gtgatcgtca ttcccggcct cgatcggctg ctgacgcgcg
     5161 agccgtcggc cgccgaacct tctacgcagc cgccagcgag caaggcatga tcgagctgct
     5221 ctcccgcctg ctgggccgcg gctcgcgagc cgacgcaggc ctccagcgac tggccgcgat
     5281 tctgtccgtg cccgccgcgg agctggccgc ggtccccggc aacccgcggc tctgctaccg
     5341 gcagttcgaa atccgcaagc gcagcggccg aattcggcag atcgccgcgc cgtccggagc
     5401 gctcaagttg caccagcggc gactgctcaa gcagtacctg tcgcagcaat acgttcacgc
     5461 tgcggctaca gcgtttcgct cgggctcgtc gatcgccacg cacgcccggc gacatttagg
     5521 ccagcaaatc gtgttgactg tcgatctggc cgactttttt cccgcgaccg ccgcccgccg
     5581 cgtccgtcgt tggttccgcg agcagggctg ggaaggcgcg gcgctcgacg tgctgatgcg
     5641 gctgacggtc tatcgtggcg gcctgccgca aggcgcgccg accagccccg cgctgagcaa
     5701 cctcgtcaac cggtccctgg acgaggagct gagcgagctg gccgggcaat ctggcggccg
     5761 ctattcccgc tattgcgacg acctggcctt ctcgttcaga actgaagccg agcccatgac
     5821 cctccgcccg caagtcgagg cttgcctggc tcgccacggc tatgaagtcc aagcggaaaa
     5881 gggctggcgt ttgcaggccg ccgccgagca tccagaaatt acgggcctgg tactcgccgg
     5941 ccgccgactg cgcctggccg accacatcct ccgccgcatc caggccctcc gcacgcgctg
     6001 gttcggcctc tccgcgaacg accgccagcg cctcgccggc taccgcggcc tggcgaaaat
     6061 gatcaagcga tagggccgcc ggctactcga gcgcgcccga gtcgcgccat ttcttgatca
     6121 tgccgcggag cttcttctca tcgggcgttt cgtcgctgcc ttcgaagacc gacgtgatgc
     6181 tggcgtacgc cgatttccat gcgcccgttt cggcgtcctt ctcgaacgac tggatgtcgt
     6241 gcttgcggat gccgttctct tcgtaggaaa tgaagaggat ctttttgccg cggaattcgg
     6301 actccttgcg gaactcgagt gtccgcagtt tgtcctgggc ccgcttggcg ttgaactggt
     6361 cggccgactc cagaatcgcc cggtagtcct ggctctcgag catcttcagc tgcgacacca
     6421 acgcggcttt ggggctgctc aggtcgtagg cggccagtcc gcggcccagc ggatcgtgcg
     6481 agaacgtcca gcaggcaact ccgcacgaaa tggccagggc cacgcccgac aggatcgtgg
     6541 ccagcggcac ggcgggctgc ttggccggcg acgacggcgg cgcaccgcgt tccaggactt
     6601 gcgacatggc gtgagactcc gagcttgttt ggaagggaca gcaaccctag ctcatcgccg
     6661 ccgggcgcgc cgacgcgcct ggaccacaaa tccgctacgg cccgcgcaag ctgctgaaag
     6721 tgtggcggtc aggaatgtct gccccacggt cgccctgcac ggcgaaaggg cgttccgcta
     6781 aaatccgccg agagagcgcc gcgtggacgc ggcggctaag cgggtctgct gcctgaaccc
     6841 tgaaccccga accctgaact cacaccaatg gccaaaacgg caatcacccc cactcgcgcc
     6901 gaagactacc cgga
//
LOCUS       DASRJB010000855         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3917222, whole genome shotgun sequence.
ACCESSION   DASRJB010000855 DASRJB010000000
VERSION     DASRJB010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917222"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFB80_22475"
     CDS             <1..819
                     /locus_tag="VFB80_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36620.1"
                     /translation="PARAITRVGHIDWSTVNPLPGSCRWSRPACSPGRFLRPSQLLIM
                     TLACGVGLAWLGNKVRDVREQRQILRQVFAGGFSVGVTDWYSDRMPRWVYRWFGLSAP
                     IATDSLGCVCNNKVVRDEDLRALAGLRFCELSLNSSNVTDDQIVRAPLPANLKCFSAQ
                     GTALGDRALAHLADCQSLEAVELYGVQVTDAGVAHLVRLPNLYIIGLEKTSLTDGAVE
                     HLKKMKHLRALRIWQTNITPRSMNELRDNLPDCVIVDDSRQATQENCFYWPEKP"
     gene            complement(834..1505)
                     /gene="aat"
                     /locus_tag="VFB80_22480"
     CDS             complement(834..1505)
                     /gene="aat"
                     /locus_tag="VFB80_22480"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZN36621.1"
                     /translation="MQNAREKPRFPPHDAAGPDGVLLVGGSLAPDWMLEAYRRGIFPM
                     PIVIDRRRLIAWLTPDPRGILEFDGLHVSRRLARRLKRGEFQFTLDRNFTEVVEACAA
                     PRGYDDDCWLTLAMQNAYQTLHRLGHAHSVEVWQDGWLVGGLFGVSIGGLFAGESMFH
                     RATDASKAALTFLLPHLKRRGYTLFDVQWTNDHTRSLGAIDIPRTQYLARLAEAVKRP
                     ATFGS"
     gene            1642..2976
                     /locus_tag="VFB80_22485"
     CDS             1642..2976
                     /locus_tag="VFB80_22485"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN36622.1"
                     /translation="MLDDSTTPRDQPAAEPPPAEAAAAQPAPHPRLARAVEMSLEPLV
                     VRAHPLDRPEIESRQARDENQRIRLAWARLVWLLSFLAVLLAISYLVPYMAEQTQYSI
                     TRGKQRAEHDFAKAHVGESPLAEMSRAYQMVSQVVGPSVVHINTQGRASSRILPLSTM
                     SRTRIPTDGQGSGVIVESTGYILTNHHVVKGAGSIEVALADGRHVPAKIVGWDGPTDL
                     AVLKITADLLTPAEFGDSEEIEPGALVWAVGSPFGLERSITSGILSAKHRAGLAGTHF
                     HGDFLQTDAAVNPGNSGGPLVDSQGRVIGINTAIVGESYQGISFAIPSKLARDMYERI
                     KAEGIIRRGWLGVELSEISLEEAQALGLPGGKGVKVASLYQQDGGSPAAQAGILPGDV
                     IVRWNDVPVNSPSDLSGAVGKTAVESRAKVAVWRDGKEQTFEVVVGERPILE"
BASE COUNT          554 a          980 c         1068 g          519 t
ORIGIN      
        1 ccggcccgcg ctataactcg cgtcggtcac atcgactgga gcacggtaaa tccgttgccg
       61 ggatcctgtc gctggtcgag acccgcttgc tcaccaggcc gatttttgcg cccctcccag
      121 ctgctgatca tgaccttggc gtgcggcgtc ggcctcgcct ggctgggaaa caaagtccgc
      181 gatgtgcgag agcagcggca gattctccgg caggtctttg cgggaggctt cagcgtcggc
      241 gtcaccgatt ggtactccga tagaatgccg cgctgggtgt accgctggtt cggccttagc
      301 gccccaatcg ccacggacag tctcggctgc gtttgtaaca acaaggtagt tcgcgacgag
      361 gacttgcgag cactggcagg acttcgcttt tgcgagctgt cgctcaattc ttcgaatgtg
      421 acggacgatc agatcgttcg cgcgccgctg cctgccaatc tcaagtgctt ttcggcccag
      481 ggaaccgcgc taggtgaccg cgcgctggcg catctcgccg attgtcagag cctcgaggcg
      541 gtcgagctgt atggcgtgca ggtgactgat gccggagtcg cgcacctggt ccgcctgccg
      601 aacttgtaca tcatcggctt ggaaaagacc tcgcttaccg acggcgccgt cgagcatctg
      661 aaaaagatga aacaccttcg ggcgctgcgc atctggcaaa ccaacatcac gccccgaagc
      721 atgaatgaat tgcgcgacaa cctgcctgac tgcgtgatcg tcgacgactc tcgccaagca
      781 acgcaggaaa actgcttcta ctggccagag aaaccgtgag gccggcagct gtcttagctg
      841 ccaaacgtcg ctggccgctt cacggcctcg gccagccggg cgaggtattg cgtccgcggg
      901 atgtcgattg caccgaggct gcgggtgtgg tcgttggtcc attggacatc gaacagcgtg
      961 tagccgcggc gcttcaggtg cggcagcagg aacgtcaggg cggccttgga ggcgtcggtg
     1021 gcgcggtgga acatcgattc gcccgcgaac agcccgccga tggacacgcc aaacaggccg
     1081 ccgacgagcc agccgtcctg ccagacctcg acgctgtggg cgtggcccag gcggtgcagc
     1141 gtctgatacg cgttctgcat ggccaaagtc agccagcaat cgtcgtcgta gcctcgcggg
     1201 gccgcgcagg cctcgacgac ctcggtgaag ttccggtcga gcgtgaactg gaattccccg
     1261 cgcttcagac ggcgagccag gcggcgcgaa acgtgcaggc cgtcgaattc aaggatgccc
     1321 cgcgggtcgg gcgtcagcca ggcgatcaag cggcggcggt cgatcacgat cggcatcggg
     1381 aagatgccgc ggcggtaggc ctccagcatc cagtcggggg ccagcgaccc gccgaccagc
     1441 agcacgccgt cgggaccggc cgcatcgtgg ggcggaaaac ggggcttttc gcgggcgttt
     1501 tgcatggcga gggtgccggc aatgtcaaga ataacacccc gccggcgaaa gagaatgcgg
     1561 atcggcgtca aggctgccag cattttctgg caggcggatt gacccttcgc gtataatgca
     1621 gcggtctatc gggaaaccag catgctcgac gactcgacga ccccgcgaga tcagcccgcc
     1681 gccgagccac cgcctgcgga agcagccgcg gctcaacctg cgcctcatcc gcggctagcg
     1741 cgggccgtgg aaatgtcgct cgagccgctg gtcgtgcggg ctcatccgct ggaccggccg
     1801 gaaatcgaat cccgccaggc gcgggacgag aaccagcgga ttcgcctggc ctgggcccgg
     1861 ctggtctggc tgctgtcgtt cctggcggtg ctcctggcga tcagctacct cgtgccctat
     1921 atggccgagc agacacagta ttccatcacc cgcggcaagc agcgggcgga gcacgacttc
     1981 gccaaggccc acgtcggcga gtcgccgctg gccgaaatgt cgcgggccta tcaaatggtg
     2041 tcgcaggtcg tcggccccag cgtcgtgcac atcaacaccc agggccgggc gagcagccgc
     2101 atcctgccgc tctcgacgat gtcccgcacg cggattccga ccgatggaca gggatcgggg
     2161 gtgatcgtcg aatcgaccgg ctacatcctg acgaaccacc acgtggtgaa gggggcgggc
     2221 agcatcgagg tggcgctcgc cgacgggcgg cacgttccgg ccaaaatcgt cggctgggac
     2281 gggcccacgg acctggccgt tttgaagatc acggccgatc tgctgacgcc ggcggagttc
     2341 ggcgatagcg aggagattga gccaggggcg ctggtctggg cggtgggcag cccgtttggc
     2401 ctggagcgct cgattacgtc gggcatcttg agcgccaagc accgcgcggg gctggccggc
     2461 acgcattttc acggcgactt tttgcagacc gacgcggcgg tgaatcccgg caacagcggc
     2521 ggcccactgg tggatagcca gggccgcgtg atcggcatca acacggcgat cgtcggcgag
     2581 tcgtaccagg ggatcagctt cgccatcccc agcaagctgg cccgcgacat gtacgagcgg
     2641 atcaaagccg aggggatcat ccgccggggg tggctgggcg tggagctgag cgagatcagt
     2701 ctggaggagg ctcaagccct cggcctgccc ggcggcaagg gagtgaaggt ggccagcctc
     2761 tatcagcagg acggcggctc gccggccgcg caggccggga tcctgccggg tgacgtaatc
     2821 gttcgctgga atgatgtgcc cgtgaacagc cccagtgacc tgagcggtgc ggtcggcaag
     2881 acggccgtcg aatcgcgggc caaggtcgcc gtgtggcgcg acggcaagga acagaccttc
     2941 gaggtcgtcg tcggcgagcg gccaattctg gaatagcccg ccgcgcttga catcgcgtgc
     3001 cgatgcggta cgttgagcgc tggttgggcg aagcgcaaaa tctcatcggt gccttcgccg
     3061 cggccaacag gggcaatgct cgtcgaggag agggcgatgg ccgtctgccc ccatattcgc
     3121 t
//
LOCUS       DASRJB010000856         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1106129, whole genome shotgun sequence.
ACCESSION   DASRJB010000856 DASRJB010000000
VERSION     DASRJB010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106129"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VFB80_22490"
     CDS             complement(<1..1710)
                     /locus_tag="VFB80_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN36623.1"
                     /translation="MATLFVMQGRDKGKRYDLRGADLSLGRDGTNRIQLNDSEVSRRH
                     AEVRKDDEGFLLTDLGSSNGSYVNGERVAERRLVNGDRVQIGRTLLLFTDADHLSSGN
                     LHHAVNIVGQNDVEGSRILKSVSHDSGSQIFAPAAWEAPEAADSPWLARARSNLQIMY
                     RTALAVSHTLDIDQLLARLMDLIFEWVEADRGCVMLVDHETGELTPAASRNRKGVKAD
                     ERMAISRTILDYVLSRKEGVLTSDAKDDERWNSDGSIVSLGVREAICVPMQGRYGIVG
                     VIYIDTFTPPALTVPVRTLSGMGSAVNKFSEEHLKLMVAIGHQAALAVEDTSYYSAML
                     QAERLAAMGQTIATLSHHIKNILQGIRGGSYLIEEGLQGEDNEVVRRGWRIVEKNQER
                     IGNLVMDMLTFSKDREPELAPASLSETVGDVVELMKTRAAEANVQLAWQPREGLPQFS
                     FDADLLHRAILNVVTNAIDACEKREAARVDVSIEWSPGDRLVHIVVADNGEGIAPEDV
                     KRIFSVFESKKGARGTGLGLPVSQKIMREHGGDIRVESTPGVGSRFLLELPLQIQNSE
                     FRTQ"
     gene            complement(1922..2278)
                     /locus_tag="VFB80_22495"
     CDS             complement(1922..2278)
                     /locus_tag="VFB80_22495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN36624.1"
                     /translation="MPGSVLIVDPSAENREVLRTVLARRGLVIFEAEAAEAGIELARE
                     HHPDVIVLDVQAEEADPAALRERFSAGSQDHPAELIVLGKIRRGPQFAGGHVISKPYH
                     FAPLVHTIQQLAAKAA"
     gene            2426..3361
                     /locus_tag="VFB80_22500"
     CDS             2426..3361
                     /locus_tag="VFB80_22500"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN36625.1"
                     /translation="MSRLLRAVRHLAEYLLVRVFLSLIQAVSLETCQSVTRFVAWIAC
                     DVLNIRGAVIAENLRTAFPEKTDAERRRIARGMWEHLLLMVCEVAQLNRKIHETNWRN
                     YVDVTDKRDWIRLASRAGAKVCVTGHFGNFEALGHVCSFWGFRTYTVARTLDNPYLHE
                     LVNRFRESMGQHMLSKDHSAGQAEEVLASGGFLGLLGDQHAGARGCVVDFLGRPASCH
                     KALALFTLLNRAPIMVVTCARGERPMHFRIRLDGVIDPAANPPEAESVEDLTQWYNDV
                     LAARIRQQPDQYWWLHDRWKEVKPRKRRKRRAAAA"
     gene            complement(3421..4155)
                     /locus_tag="VFB80_22505"
     CDS             complement(3421..4155)
                     /locus_tag="VFB80_22505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZN36626.1"
                     /translation="MSCWQQATQGISIAESPAAPGWIERVIRASGVRRAYWNWATRQA
                     RQRPYLQMLARCARLEGDIVECGVFRGQSLLRMAAWLQQQGIRKTVHGLDSFTGFPRE
                     EVGASDLAPGRSLRAVRGRFRTAASAPRRILRVSEDLELPIRLHVGYFDETLPRLIAS
                     GRRLCFAHLDCDIYESYRFCLEQLYSAMAPGGVILFDEYRSPSWPGATRAIDEFFAAR
                     PEKPQICRDAERPAKPKYFVVKVSGQ"
BASE COUNT          740 a         1461 c         1371 g          774 t
ORIGIN      
        1 ctgagttcgg aattctgaat tctggatctg cagcggcagc tcgagcaaaa agcggctgcc
       61 cacgccgggg gtgctctcga cgcggatgtc gccgccgtgc tcgcgcatga tcttctggct
      121 caccggcagg cccaggccgg tgccgcgggc gcccttcttc gactcgaaga ccgagaagat
      181 tcgcttgacg tcttccgggg cgatcccctc gccgttgtcg gcgacgacaa tgtgcaccag
      241 ccgatcgccg ggcgaccatt caatcgagac atcgacgcgt gcggcctcgc gcttctcgca
      301 ggcgtcgatg gcattggtca cgacattcag gatcgcccgg tggagcaagt cggcgtcgaa
      361 cgaaaactgc ggcaggccct cgcgcggctg ccaggcgagc tgcacattcg cctccgcggc
      421 gcgggtcttc atcagctcga ccacgtcgcc gaccgtttcg ctcaggctgg ccggcgcgag
      481 ctccggctcg cggtccttgc tgaacgtgag catgtccatc accaggttgc cgatccgctc
      541 ctggtttttc tcgacgattc gccagccgcg gcggacgact tcgttgtcct cgccctgcag
      601 gccctcttcg atcaggtagc tgccgccgcg gatgccttgc aggatgttct tgatgtggtg
      661 cgagagcgtg gcgatcgtct ggcccattgc ggccagccgc tcggcctgga gcatcgccga
      721 gtaatacgac gtgtcctcca ccgccagcgc cgcctggtgg ccgatcgcga ccatcagctt
      781 caggtgctcc tcggaaaact tgttgacggc actgcccatt ccgctcagcg ttcgcaccgg
      841 caccgtcaag gccggcggcg taaacgtgtc gatgtaaatc acgccgacga tgccgtaccg
      901 cccctgcatc gggacgcaga tcgcctcgcg aacgcccagc gacacgatcg agccgtcgga
      961 gttccaccgc tcgtcgtcct tggcatcgct ggtcagaacg ccctccttcc gcgacagcac
     1021 gtagtcgaga atcgtgcggc tgatcgccat ccgctcgtcg gccttcacgc ccttgcggtt
     1081 gcggctggcg gcgggcgtca gctcgcccgt ctcgtgatcg acgagcatca cacagccgcg
     1141 gtcggcctcg acccattcaa agatcaggtc catgaggcgg gccagcagct ggtcgatgtc
     1201 cagcgtgtgg ctgacggcca gggccgtgcg atacatgatc tgcaggttgc tgcgggcgcg
     1261 ggccagccag gggctgtcgg cggcctcggg cgcttcccaa gccgccgggg cgaaaatctg
     1321 gctgcccgaa tcgtggctga cggatttcag gatccgcgag ccttcgacgt cgttctggcc
     1381 gacgatgttg acggcgtggt gcaggttgcc gctcgacagg tggtcggcgt cggtgaacag
     1441 cagcagcgtg cggccgatct gcacgcggtc gccattgacc agccgccgct cggcgacgcg
     1501 ctcaccgttg acgtagctgc cgttggagct gcccaggtcg gtcagcagga agccttcgtc
     1561 gtccttgcgg acttcggcgt ggcggcggga gacttcgctg tcgttgagct ggatgcggtt
     1621 ggtgccgtcg cggcccaggc tcaaatcggc tccgcgcagg tcgtagcgct ttcccttgtc
     1681 gcgcccctgc atgacgaaca gcgtggccat acttacccat cgtagaacaa gcgtccgcgc
     1741 ttgttcaccc tagtagtgca actcgtccat ttgtgtgggt cgcgcccatt ttgcaatttg
     1801 cacttcgcaa tttgcacttt gcaattcagc aggggatcgc agaacctcgc cactccggcg
     1861 cattgcaaag tggcaaatgc aaattgcaaa ttgcaaagtg ttgccgcgag gacgagtggc
     1921 actaggccgc tttggctgct agctgctgga ttgtatggac cagcggagcg aagtggtagg
     1981 gtttgctgat cacgtggccg ccggcgaatt gcggcccgcg gcggattttg cccagcacga
     2041 tcagctcagc cggatggtcc tgcgagccgg cgctaaaccg ctcgcggagg gccgccggat
     2101 cggcctcttc ggcctgcacg tcgagcacga tcacgtccgg atggtgctcg cgagccagct
     2161 cgatgccggc ctcggcggct tccgcctcga aaatcacgag gccccggcgc gccagcaccg
     2221 tccgcagcac ttcgcggttc tcggccgagg gatcgacgat cagcacgctg ccaggcatag
     2281 gccgcggaga atatcggccc cgccgccgcg tgtcaaaacc gaaaatcgct cttggctgcc
     2341 agcagcgggc tggggatagt agtcgtctcg ctccgccaga cgccgcctca cgcggagcgt
     2401 gaggcctact tggaaggaca acggaatgtc ccgcttgctt agggccgtgc ggcacctggc
     2461 cgaatacctg ctggtccgcg tcttcctgtc gctgatccag gcggtgagcc tggagacttg
     2521 ccagagcgtc acccgcttcg tggcctggat cgcttgtgac gtgctcaata tccgcggcgc
     2581 ggtcattgcc gaaaatctgc gcaccgcctt tcccgaaaaa accgacgccg agcggcggcg
     2641 gatcgcccgc ggcatgtggg agcacctgct gctgatggtc tgcgaagtgg cgcagctcaa
     2701 ccgcaagatt cacgaaacga actggcggaa ctacgtggac gtcaccgata agcgcgactg
     2761 gattcgcctg gccagtcggg ccggggcgaa ggtctgcgtc acgggtcact tcggcaactt
     2821 cgaggccctg ggccacgtct gcagcttctg gggcttccgt acctacaccg tggcccgcac
     2881 gctcgacaat ccctacctcc acgagctggt caaccgcttc cgtgagtcga tgggccagca
     2941 catgctctcc aaggaccaca gcgccggcca ggccgaagag gtgctcgcca gcggcggctt
     3001 cctcggcctg ttgggcgatc agcacgcggg ggcccgcggc tgcgtcgtcg attttctcgg
     3061 ccggcccgcc tcgtgccaca aggccctggc cctgttcacg ctgctcaatc gggcgccgat
     3121 catggttgtc acctgcgccc gcggcgagcg gccgatgcac ttccgcatcc ggctcgacgg
     3181 cgtgatcgac ccggccgcca atccgcccga agccgaaagc gtcgaagacc tcacgcagtg
     3241 gtacaacgac gtcctcgctg cccgcatccg ccagcagccc gaccaatact ggtggctcca
     3301 cgaccgctgg aaggaagtca agccgcggaa acgccggaag cggagagcgg ccgccgcata
     3361 ggaattgtga gtggtcagtg gtcagttgtc agttggaagt gccaaccaac tgacaactga
     3421 ctactgacca ctgaccttca ccacaaaata cttcggcttc gccggccgct ctgcgtcccg
     3481 gcaaatctgc ggcttttcgg gccgcgcggc aaagaattca tcgatggccc gcgtcgcgcc
     3541 gggccaggat ggactgcggt actcgtcgaa caggatcacg ccgccgggcg ccatcgcgct
     3601 gtaaagctgc tccaggcaaa agcggtacga ctcgtaaatg tcgcagtcga ggtgcgcgaa
     3661 gcaaagccgc cggcccgagg caatcagccg cggcagcgtc tcgtcgaaat agccgacgtg
     3721 cagcctgatc ggcagctcca gatcctctga cacgcgcagg attcgccgcg gcgcgcttgc
     3781 ggccgtgcgg aaccgcccgc ggaccgcccg cagcgagcgg ccgggagcaa ggtcgctggc
     3841 gccaacctcc tcgcgcggaa acccggtaaa gctgtccagg ccgtggaccg tcttgcggat
     3901 gccctgctgc tgaagccagg ccgccatgcg gagcagcgac tgcccgcgaa agacgccgca
     3961 ctcgacgatg tctccttcca gccgcgcgca gcgggcgagc atttgcaggt acggccgctg
     4021 ccgcgcctgc cgcgtcgccc agttccaata cgcccggcgg actccgctgg cccgaatgac
     4081 tcgctcgatc catcccggcg cagccgggga ctcggcaatc gagattccct gtgtcgcctg
     4141 ctgccagcac gacatcctcg accttccttt cgggcggact ataaccgcgc acaaatgaga
     4201 ccagtaagag cagtttcaaa cagaatacca ttggcggata cccttaaccc tcgccggcga
     4261 atataaggaa aactgccaac tggccgcatc cttgcacggg ccctacgcgc ggctgatcgc
     4321 accataactg cttggccgtt taggac
//
LOCUS       DASRJB010000857        10148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1944827, whole genome shotgun sequence.
ACCESSION   DASRJB010000857 DASRJB010000000
VERSION     DASRJB010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944827"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFB80_22510"
     CDS             <1..453
                     /locus_tag="VFB80_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36627.1"
                     /translation="KLRQLDAPGFYTWDPKQRIHSIGGTRAVTFSPDGKQLVAGGIGA
                     IGNIDHLEGPARVEVFNLETGASEHVFSGDAKGLVEQLLFHPEGKWLLAVGGDNGGLI
                     QAYDLAEKKVTRSDKAPMHIHAAALNDDATRLFAAGHGKIAVWEIAST"
     gene            770..1336
                     /locus_tag="VFB80_22515"
     CDS             770..1336
                     /locus_tag="VFB80_22515"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HZN36628.1"
                     /translation="MIDWTSVFEEAYPAPGASAGDLQRFTASVGQPLTAAEIAALHRG
                     QSNPFPKSDPLYASWRPFDASIWQVPDRPLPATYLRLLACSNGGGFRTGERWFQFFPI
                     LDPGQGVRAMLLAYHLPRYMPGALPVAFNGGGTFYLFDMRLPARDGEYPIVCSHSGDL
                     GWQPDQCLQIAESFEMACRGTVNVDELR"
     gene            complement(1348..2496)
                     /locus_tag="VFB80_22520"
     CDS             complement(1348..2496)
                     /locus_tag="VFB80_22520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZN36629.1"
                     /translation="MSEDSPSPPTRFGSGWISGVLSVLLSGLGLGAVLCIQNWDLLTM
                     IEAREHYARYLPVLRGVVHVVLVAGFLLGVVSVTLRQWKYLGLAGCCLVFVAALLGGS
                     QVELADRPGKPFYLGLDYFLLSLIGTAAIFVPMERLFGRREQPIFRSGWKTDLSYFFI
                     STLAVQISTLLTMKPAMVFFAWAQFESVQSFVRSLPWVLQFFLIMLSTDIVQYWVHRV
                     FHMVPWLWKFHAVHHSTDAMDWLAGSRLHIVEQVVVRALSFLPVFILGFAEPPMIAYV
                     IFITFHATFIHANVRWDYGPFRWLLATPKFHHWHHAIEREAIDKNFAVHFPLLDLLFG
                     TFYLPDQWPSGYGVHDKVPRGWLRQLVYPFLPAKTSLAEGTPTVQVHE"
     gene            complement(2493..5231)
                     /locus_tag="VFB80_22525"
     CDS             complement(2493..5231)
                     /locus_tag="VFB80_22525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN36630.1"
                     /translation="MPAVSPPIAVPAPHTEFSGSQPPGPACSEALWMLDPSGRVVHWS
                     AAAEQLTGYAAAQIVGEPFTRLTRQDEPAAAQRLLDAALVSGRAEASGILTRSGESLA
                     ATTAILPLVNRQGQSISFACLTRATPPCPHHSAGRPPSLTGDELFRALVEHGFEGINL
                     LDAEGQVIYSSPGNRRVFGYDADETDGQSGFAFAHPDDVAAAAQAWRSMLEHPQQVFT
                     TRIRVRHKDGSWRWTEATVRNLLHVPAVRGVVVNWRDVTLQHLAETELQRTSDLLRAV
                     ADNTTDAVFVKDVGGKYLLFNDAAGRFVDRQPAEVLGHDDTHLFDPASAAAVMARDRQ
                     VLESGVVSTAEEQLTAAGVTRVYHSTKSPYRNASGKICGLIGIARDITASKQAEQALR
                     DSEERYRRLVELLPDATYINCQDRIVFCNPAFVRLTGATDEQQVLGRSPFEIFPPNTH
                     ELIRDRVRRMMEQGQSVPLVEVELLRVDGSPVAVHVAATPISHHGQPAILVVLHDLTE
                     LRRSEDERKRLEEQFRQAQKMEAIGRLAGGVAHDFNNLLTVILGFSDLLLTEHPVDGN
                     SRASLMAIRAAGERAAALTRQLLAFGRKALIEPQLLDLNAAVAQIDNMLRRLIGEDIA
                     LRLALAPELPPVRVDPGQIEQVLMNLVVNARDAMPGGGTLTIATEPVVIGTAAVARLA
                     LAPGAYVQLTVTDTGQGMDAATQLRIFDPFFTTKDEGRGTGLGLAVVHGIVQQSGGQI
                     DVESQLGRGTTFRVWLPAASDSPAAVPLTGMHAEEPAPDERGNETILLVEDEDAVRTI
                     ASLALEARGYRVLAAAGGRQALRLVQSLSPPPNLLVTDVVMPGLGGPQLAASLQADFP
                     NLKVLYLSGYTNDAAVVPGLPSDGAGFLSKPYTARALAHKVREVLGQRHATPTSVP"
     gene            complement(5270..5731)
                     /locus_tag="VFB80_22530"
     CDS             complement(5270..5731)
                     /locus_tag="VFB80_22530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN36631.1"
                     /translation="MTGPAGPLGAAGSFSAGESMPPVESDADFFAKAGPLPTAWDDSR
                     RFPRFYYRAQVQATIHPFRGAPQPPVQCSVLTRDLSRGGLNIMHSDQLFPGQRIELVL
                     LDGVRRLVEVCWCRRLGKGSYSVGCRFLPSPAPKPAPPPGDSSPTGAQPAD"
     gene            complement(5728..6693)
                     /locus_tag="VFB80_22535"
     CDS             complement(5728..6693)
                     /locus_tag="VFB80_22535"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN36632.1"
                     /translation="MAVTSLESRGYLPVATAALCPASVLDCDLYLQRPGSPHAELYRG
                     SSYPLEATDLDRLRADGVEHLYIRAEDAEAFRHYLCHNVLHDRSVPTTTRIKALREVT
                     RVSFQDALTGNDCNKLVDVATEFGRDLACVVIERHIVFRELFTTLEHDFYTFTHVCNV
                     AMYCSALASRLWMSSAEELAELAAGALLHDIGKRHISPKILNKAGKLTEEEWVQVREH
                     PVSGFRELMGRGDLSWPQLMMVYQHHERLDGSGYPSGITGDEMQPWARICAVADVFDA
                     LTCQRPYRRAQPLADVCDYLQKHAGKWFDAEAVECWVSHVRSVVP"
     gene            complement(6940..7746)
                     /gene="purQ"
                     /locus_tag="VFB80_22540"
     CDS             complement(6940..7746)
                     /gene="purQ"
                     /locus_tag="VFB80_22540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HZN36633.1"
                     /translation="MTPRVLILRAPGTNCDQETAFAFQQAGGKPEVVHLNRLLENPRL
                     ASNYQILAIPGGFSYGDDISAGRIFGNQMRHHLKECLAEFKAAGKLIIGICNGVQILV
                     KSGVLLPDRADEPIATLTLNESGKFEDRWVHLRVASDKCVFLRGIERMYLPVAHAEGK
                     FVARDEATLKRLEAAGQLVLRYTMAPDFRNATEGVPYTDQPTTVPYPANPNGAQLDVA
                     GLCDETGRVFGLMPHPERHIDWAQHPRWTREPRERGDGLAVFENAVRFFG"
     gene            7860..9257
                     /locus_tag="VFB80_22545"
     CDS             7860..9257
                     /locus_tag="VFB80_22545"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN36634.1"
                     /translation="MTAADDFEDLECIGRGGFGEVYRCRRASDGKIFARKRILAFARR
                     VSASDDVRRFIREVRLLSRLSHPNIIPIVAANTSSQPFWYVTPLYDHSLLTFLPEIVC
                     RHEQIHSVVAKVMAGVGFAHKNGILHRDLSLGNVLLNSLDDVVVCDFGLARMLDDFDS
                     TRTQSLQFFGTRYFMPPETQDLGSKYADERGDIYSIGRLIVALYLGVLKHSFQGVIGL
                     PEAVRECVNSLATDYDARLETMEEAEAAWGGVPVALTNWESFSLDLLRSAVFYVDGGN
                     CEAALNDAIRHIESQRLFRMTAADPHEEVPRALASHYASFARAIFSFVCVAPLPMLLY
                     FQRARPKLFVQLLGHAGRFLADSVDELPALFRRDMIARAHELFAQIERLEVRLELMII
                     ALTILTAQPGKRATAENVVNSMFTEPFESSEIAVLCKGLRSWWKKRGSARCEMPAVRV
                     GEFAEPFQDFFAGCR"
BASE COUNT         1669 a         3309 c         3434 g         1736 t
ORIGIN      
        1 aagctgcggc agctcgacgc gccgggcttt tatacctggg atccgaagca gcggatccac
       61 tcgatcggcg gcacgcgggc cgtgacattc tcgcccgacg gcaaacagct ggtggccggc
      121 ggcatcggtg cgatcggcaa catcgaccac ttggagggcc ccgcgcgggt cgaggtcttt
      181 aatctcgaaa ccggcgccag cgagcacgtt ttctcgggcg acgccaaagg cctcgtggag
      241 cagctgctgt ttcacccgga aggcaagtgg ctgcttgccg tcggcggcga caacggcggc
      301 ctgatccagg cctacgacct ggccgagaag aaggtcacgc ggagcgacaa ggccccgatg
      361 cacatccacg ccgccgcgct caacgacgac gccacccggc tcttcgccgc cgggcacggc
      421 aagatcgccg tctgggaaat cgcctcaacc tgacacgatc caatccacta ctgtggcaag
      481 gcggcaggcc ctatcgctgg ccgactggtt cgacaactgc gacccctggg atcctggagg
      541 tgcgttttcg accggccatt cttctgagcc atcctgttca tcccgctcgt gatcgcgctt
      601 gcctggccgg cgcgctcgtt gccctgctgc gcccgccgtg gcattgcgcc ctggcggttt
      661 acgtgctgtg caatgtgctc gtattcggcg tgctaatttg gtgggagcgc cggccttcac
      721 tacggcgatg acggtcgtcg gctgggtgat gacgctacgg ggatcgccga tgatcgactg
      781 gacgtccgta tttgaagaag cctatccggc gccgggtgcg agcgccggcg atctccagcg
      841 cttcactgcc agcgtgggtc agccgctcac tgcggccgag attgcggcgc tccatcgggg
      901 gcaatccaat cccttcccca agagcgatcc gctgtatgca tcgtggcggc cgttcgatgc
      961 gtcgatctgg caagttccag accgcccctt gccggcgacg tacctgaggc tgctggcgtg
     1021 ttccaacggc ggagggttcc gcacgggcga gcgctggttt caattcttcc ccatactcga
     1081 ccccgggcag ggcgtccgcg caatgctgct ggcttaccac ctgccgcgat atatgcccgg
     1141 cgcgctgccg gtggcgttta acggcggagg aacgttctac ctgttcgaca tgcggctgcc
     1201 agcacgggac ggtgaatatc cgatcgtctg ttcgcactcc ggcgacctcg gctggcaacc
     1261 ggatcagtgc ctccagatcg cggaatcgtt cgagatggcg tgtcgcggta cggtgaatgt
     1321 cgatgaattg cgatagctca gcccaaccta ctcgtgcact tgcacggtgg gcgtgccttc
     1381 ggcgagggag gttttggcgg gcaggaaagg atagacgagc tgccgcagcc agccgcgggg
     1441 gactttgtcg tggacgccgt agccgctggg ccactggtcg ggcagataga acgtgccgaa
     1501 cagcaggtcg agcagcggaa agtgcacagc gaagttcttg tcgatcgctt cccgctcgat
     1561 ggcgtggtgc cagtggtgga acttgggcgt cgccagcagc cagcggaagg gaccgtagtc
     1621 ccaccgcacg ttggcgtgga tgaatgtcgc gtggaacgtg atgaagatca cgtaggcgat
     1681 catcggcggc tcggcgaagc cgagaatgaa gaccggcagg aacgacagcg cccgcacgac
     1741 cacctgctcg acgatgtgca gccgcgaacc ggccagccag tccatcgcgt cggtcgagtg
     1801 gtgcacggcg tggaacttcc acagccaagg gaccatgtgg aacacgcggt ggacccagta
     1861 ctgcacgatg tcggtggaga gcatgatcag gaagaactgc agcacccagg gcagcgacct
     1921 gacaaacgac tgcacgctct cgaattgggc ccacgcgaag aagaccatcg ccggcttcat
     1981 cgtcaagagc gtcgagattt gcaccgccag cgtactgatg aagaagtagc tcaggtcggt
     2041 cttccagccg ctgcggaaga tcggctgctc gcggcggccg aagagccgct ccatcggcac
     2101 gaagatcgcc gcggtcccga tcaggctgag caggaagtaa tcgaggccca ggtaaaaagg
     2161 cttgcccggc cggtcggcca gctcgacctg cgagccgccg agcagggccg ccacgaagac
     2221 caggcagcag cctgccaggc ccaggtattt ccactgccgc aaggtgacgc tgacgacgcc
     2281 gagcagaaat cccgcgacga gcacgacgtg caccacgccg cggagcaccg gcaggtaccg
     2341 ggcgtagtgc tcgcgggcct cgatcatcgt gagcaggtcc cagttctgga tgcagagcac
     2401 ggcgcccagt cccaggccgc tgagcaggac ggagagtacg ccgctgatcc agccgctgcc
     2461 gaagcgggtg ggtggtgagg gagaatcttc gctcatggga cggacgttgg cgtggcatgg
     2521 cgctggccga gtacttcgcg gactttgtgg gccagcgcgc gggcggtgta gggtttggaa
     2581 aggaagcccg cgccgtcgct gggcagtccg ggcacgacgg ccgcgtcgtt ggtgtagccg
     2641 ctgaggtaca acaccttgag gttcgggaag tcggcctgga ggctggcggc gagctgcggg
     2701 ccgcccaggc cgggcatgac cacgtcggtg accagcagat tgggcggcgg cgacaagctt
     2761 tgcaccagcc gcagggcctg ccgcccgccg gcggcggcga gcacgcggta gccgcgggct
     2821 tccagggcca ggctggcgat ggtgcggacg gcgtcttcgt cttcgacgag caggatcgtt
     2881 tcgttgccgc gctcgtcggg cgctggctcc tcggcgtgca tgcccgtcaa gggcacagcg
     2941 gcgggcgaat cggacgcggc gggcaaccag acgcggaatg tcgtgccgcg gccgagctgc
     3001 gactcgacgt cgatctggcc gccgctttgc tggacgatcc catgcacgac ggccaggccc
     3061 aggccggtgc cgcggccctc gtccttggtc gtgaagaagg ggtcgaagat tcgcagctgc
     3121 gtcgcggcgt ccatgccttg acccgtgtcg gtcaccgtca actgaacgta ggcgcccggc
     3181 gccagggcga ggcgagcgac tgccgcggtc ccgatgacca ccggctcggt ggcgatcgtc
     3241 agcgtgccgc cgccgggcat cgcatcgcga gcgttgacga ccaggttcat cagcacctgt
     3301 tcgatctggc ccggatcgac tcggaccggc gggagctccg gcgccagcgc caggcgcagg
     3361 gcaatgtcct cgccgatcag ccgccgcagc atgttgtcga tttgcgcgac ggcggcgttc
     3421 aggtcgagca gctgcggctc gatcagggcc ttgcggccga aggccaacaa ctgcctggtc
     3481 agggcggcgg cccgctcgcc ggcggcgcgg atggccatca gcgacgcccg gctgttgcca
     3541 tccaccggat gctcggtgag cagcaggtcg ctgaagccga ggatgaccgt cagcaggttg
     3601 ttgaagtcgt gggcgacgcc gccggccagc cggccaatgg cctccatttt ctgggcctgg
     3661 cgaaactgct cttccagccg cttccgctcg tcttcggagc ggcggagctc ggtcaggtcg
     3721 tgcagcacga cgaggatcgc gggctggccg tggtgcgaga tcggcgtggc ggcgacgtgg
     3781 acggcgaccg ggctgccgtc cacgcggagc agctcgacct cgaccagcgg cacgctctgg
     3841 ccttgctcca tcatccgccg gacgcggtcg cggatcagct cgtgcgtgtt gggggggaag
     3901 atctcgaagg gggagcggcc gaggacttgc tgctcgtcgg tcgcgcccgt caggcggacg
     3961 aacgccgggt tgcagaacac gatccggtcc tggcagttga tgtaggtcgc gtcgggcagc
     4021 agctcgacga gacggcgata gcgctcttcg ctgtcgcgga gggcctgctc ggcttgcttg
     4081 gaggccgtga tgtcgcgggc gatgccgatc aggccgcaga tttttcccga agcgttgcgg
     4141 tagggggact tggtggagtg atagacccgc gtgacgcccg cggccgtcag ctgctcctcg
     4201 gccgtgctga cgactccgga ctcgagcacc tggcggtcgc gggccatgac cgccgcggcg
     4261 ctggccgggt cgaagaggtg cgtgtcgtcg tgccccagga cctcggcggg ctggcggtcc
     4321 acgaaccggc ctgccgcatc gttgaatagc agatacttgc cgcccacgtc cttgacgaac
     4381 acggcgtcgg tcgtgttgtc ggcaacggcc cgcagcaggt cgctggtccg ctgcagctcg
     4441 gtctcagcca ggtgctggag cgtgacgtcg cgccagttca ccacgacgcc tcgcaccgcc
     4501 gggacgtgca gcaggttccg gaccgtggcc tcggtccacc gccacgagcc gtccttgtgc
     4561 cgcacgcgga ttcgcgtcgt gaagacctgt tgcggatgct ccagcatgct ccgccaggcc
     4621 tgggccgcgg cggccacgtc gtcgggatgg gcaaaggcaa acccgctctg gccgtcggtc
     4681 tcgtcggcgt cgtagccgaa gacgcggcgg ttgccggggc tggaatagat cacctgcccc
     4741 tcggcatcga gcaggttgat gccctcgaag ccgtgctcca ccaaagcccg gaacagttcg
     4801 tcgccggtca ggctcggggg ccggccggcg gagtggtggg ggcaaggcgg agtggcgcgg
     4861 gtcaggcagg cgaagctgat cgactggccc tgacgattga ccagcggcag gatcgcggtc
     4921 gtcgccgcca gggattctcc cgaacgcgtg aggatcccgc tcgcttcggc gcgcccgctg
     4981 accagcgccg catcgagcag gcgctgtgcc gcggcgggct cgtcctgacg cgtcaggcgc
     5041 gtaaacggct cgccgacgat ctgcgccgcg gcatagccgg tcagttgttc cgccgcggcg
     5101 ctccagtgaa cgacgcggcc cgacggatcg agcatccaca gcgcctcgga gcaggccggc
     5161 cccggcggct ggctgccgga gaactcggtg tgcggagcgg gcacggcgat gggcggagaa
     5221 acggctggca tgtcccagat tctgacggaa actccggcgg ctgcccagct taatccgccg
     5281 gctgagcccc ggtcggcgac gaatcgccgg gcggcggcgc gggcttcggt gccgggctgg
     5341 ggagaaagcg gcagccgacg gaataggacc ctttgcccag gcggcggcac cagcagactt
     5401 ccaccaggcg gcggacgccg tcgagcagca ccagctcgat ccgctggccg ggaaagagct
     5461 gatcgctgtg catgatgttc aggccgcccc gcgacagatc gcgagtcagc acgctgcact
     5521 ggaccggcgg ctgcggcgcg ccgcggaagg ggtgaatcgt ggcttgcacc tgggcccgat
     5581 agtagaaccg cggaaagcgg cgcgagtcgt cccaggccgt gggcagcggc cctgccttgg
     5641 cgaagaaatc ggcatccgat tcaaccggcg gcatgctttc cccggcggag aacgagcccg
     5701 ctgctcccag cgggccggcc gggccggtca tgggacgacg ctccgcacgt ggctgaccca
     5761 gcactcgacg gcttccgcgt cgaaccactt gccggcgtgc ttctgcaaat agtcgcagac
     5821 gtcggccagc ggctgggccc ggcggtaggg ccgctggcag gtgagcgcgt cgaacacgtc
     5881 ggccacggcg caaatgcggg cccagggttg catctcgtcg ccggtgatgc ccgagggata
     5941 gccgctgccg tcgagccgct cgtggtgttg atagaccatc atcagctgcg gccagctcag
     6001 gtcgccgcgg cccatcagct cgcggaagcc gctgaccggg tgctcgcgga cctgcaccca
     6061 ctcttcctcg gtcagcttgc ccgccttgtt caggattttt ggcgaaatgt gccgcttgcc
     6121 gatgtcgtgc aggagcgccc cagcggcgag ttccgccagc tcttcggcgc tgctcatcca
     6181 cagccggctg gccagggccg agcaatacat ggccacgttg cagacgtgag tgaacgtgta
     6241 gaagtcgtgt tcgagcgtgg tgaacagctc gcggaacacg atgtgccgct cgatgacgac
     6301 gcaggccagg tcgcggccga attccgtcgc cacatcgacc agcttgttgc agtcgttgcc
     6361 ggtcagggcg tcctggaagc tgacccgcgt cacctcccgc agggccttga tccgcgtcgt
     6421 cgtcggcacg ctgcggtcgt gcagtacgtt gtggcacagg taatggcgga acgcctcggc
     6481 atcctccgcc ctgatataga ggtgctccac gccgtcggcc cgcaggcgat cgaggtccgt
     6541 ggcctccagc ggatagctcg agccgcgata caactccgca tgcggcgaac cgggacgctg
     6601 gagatagagg tcgcagtcga ggaccgacgc cgggcaaagc gccgccgtcg ccaccggcag
     6661 gtagccgcga ctctccaagg aggttaccgc cataagcacc tcgggcccca cgccgcaggg
     6721 ccgattttcc cagtccagca aaccctagca ttgccccgga aagtggctga attctctgcg
     6781 gggaaacggt tgcgactggc tgtatcggct gccgccacga accggataaa acgcgcggcc
     6841 gctaaaggcg cgcggtccgg cgagtaaaga taatcgttcg ttttttggtc agttgtcagt
     6901 tgtcagttgg atttggcact ccccactgac gactgacaac tagccgaaga atctcacggc
     6961 gttttcgaag accgccaggc cgtcgccccg ctcccgcggc tcgcgggtcc aacggggatg
     7021 ctgcgcccaa tcgatgtgcc gttctgggtg cggcatcagt ccgaagacgc ggcccgtttc
     7081 gtcgcacagt cccgccacgt cgagctgcgc cccgttggga ttcgccgggt agggcacggt
     7141 ggtcggttgg tcggtgtagg gaacgccctc tgtggcgttc cggaaatcgg gagccatcgt
     7201 gtaccgcaag actagctgcc ccgccgcctc gagccgcttc agggtcgcct cgtcgcgggc
     7261 cacgaacttg ccctcggcat gggcgaccgg caaatacatc cgctcgatgc cgcgcaggaa
     7321 gacgcacttg tcgctcgcca cgcggaggtg cacccagcgg tcctcgaact tgccgctttc
     7381 gttgagcgtc agcgtggcga tcggctcgtc ggcccggtcg ggcaacagca cgcccgactt
     7441 cacgagaatc tgcacgccgt tgcagatgcc gatgatcagc ttgcccgccg ccttgaattc
     7501 cgccaggcac tctttcaagt gatgccgcat ctggttgccg aagattcgcc cggccgagat
     7561 gtcgtcgcca tagctgaacc cgcccggaat cgccaggatc tggtaattcg acgccagccg
     7621 cggattctcc agcagccggt tcaagtgcac cacctccggc ttgccgcccg cctgctgaaa
     7681 agcgaacgcc gtctcctgat cgcagttcgt accgggagca cgcagaatca aaacgcgcgg
     7741 tgtcatggaa ttattgtagc ggcgcgtcgc catttggtct gggtggcgat cagcacgttg
     7801 gctgctagac ttggcttggc ggcgcttgga tttgttccgg cttcttcagc ctcagcgata
     7861 tgaccgctgc ggacgacttc gaggatctcg agtgcatcgg ccgaggcggg ttcggcgagg
     7921 tgtatcgctg ccgcagggcc agtgacggca agatcttcgc tcgcaagcga atcctggcct
     7981 ttgcccgccg cgtctcggcc agcgacgacg tccgccgctt catccgggaa gtgcggctgc
     8041 tcagccggct gtcgcacccg aacatcatcc cgatcgtcgc ggccaacacg tcgtcgcagc
     8101 cgttctggta cgtgacgccg ctctacgacc actcgctgct gacgttcttg ccggagatcg
     8161 tctgccggca cgagcaaatt cactcggtgg tggccaaggt gatggcgggc gtcggtttcg
     8221 ctcacaaaaa cggcatcctg caccgcgacc tttcgctggg caacgtgctg ctcaactcgc
     8281 tggacgacgt ggtggtctgc gacttcgggc tggcccggat gctggacgat ttcgactcga
     8341 cccgcacgca gtcgctgcag ttcttcggca cccgctactt catgccgccc gagacgcagg
     8401 acctgggcag caagtacgcc gacgagcggg gcgacatcta ctcgatcggc cggctgatcg
     8461 tggccctcta cctgggcgtc ctcaagcaca gcttccaggg cgtgatcggg ctgcccgagg
     8521 cggtccgcga gtgcgtgaac tcgctggcca ccgactacga cgcccgcctg gagacgatgg
     8581 aggaggccga agccgcgtgg gggggcgtgc cggttgcgct caccaactgg gaatcgttct
     8641 cgctcgacct gctccgctcg gccgtgttct acgtggacgg cggcaattgc gaggcggcgc
     8701 tcaacgacgc catccggcac atcgagtcgc agcggctgtt ccgcatgacg gcggccgacc
     8761 cgcacgaaga ggtgccgcga gctctcgcat cgcactacgc ctcgttcgcg cgggcgattt
     8821 tcagcttcgt ctgcgtcgcc ccgctgccga tgctgctcta cttccagcgg gcccgcccca
     8881 agctgttcgt gcagctgctg gggcacgccg gccgcttcct ggccgactcg gtggacgagc
     8941 tgccggccct ctttcgccgt gacatgatcg cccgggccca cgagctgttc gcgcagatcg
     9001 agcgcctcga agtccggctg gagctgatga tcatcgccct cacgatcctc accgcgcagc
     9061 cggggaagcg ggccacggcc gagaacgtcg tgaactcgat gtttaccgag ccgttcgaat
     9121 cgagcgagat cgcggtgctc tgcaagggcc tccgctcctg gtggaaaaag cgcggcagcg
     9181 cccgctgcga aatgcccgcc gtccgcgtgg gcgagttcgc cgaacccttc caggacttct
     9241 tcgccggctg ccggtagcgc cgacatttgt tcgccggatc gcgacgctcg gtggcgataa
     9301 aattcggagc cgtttggcaa tattcgcagg cttttccgca cttttagccc cgagtaattc
     9361 gccaaacagg cacggtatct ggggatctgg cgattaatgc gctgccggcc gccgcaagtg
     9421 gttactgcgc cagcagttag ctgaaaaaca agggtggcga ttaattcgcc gctcgtcctg
     9481 cctctggcag tcgcgcctca ttcttcccag ggtgcagcac cgcccgtccg ctccttcatc
     9541 gaaggcgtcg ccacgctcca ccaaatttcc aatgaacacg ccgccgggcg agaacgccgc
     9601 ggctgtttgg aattgtaatt gtccgtccac tatatgtcaa gtcaaccaca acagattgtg
     9661 gtcgcctccc gccaaaccac aataggttgt ggtcgggcct ctcgacggcc cccgaaagag
     9721 ggcccggcca aaacacgaag ggcaccaaga gcacgaagag gccctggtac tattccgctt
     9781 ggtgatcttc gtgttctttg tgtttacctt tctggcgggt taaaggtgcg atgattcatc
     9841 cggatacgcc tgatgcccct tggggcaccg acgaaacccg ttttgatccg attcactcgg
     9901 ccgcccgcat gcgcgacgtt tcagccgtgc ggaaagagct cgacgccgga gtggatgtcg
     9961 atattctcca tggccgcgca gccaatggcg acggcggcaa caccgccctc tggtttgccg
    10021 cccaaggccc agcgcccttc cctggcgacg cggcaagccc tgctgacgag cgggtcaagc
    10081 gcctttcggg ccgtcctgct ctcgggccag gcccgccagg gcgaaaaagc caatagcggc
    10141 gatcccgt
//
LOCUS       DASRJB010000858         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3918619, whole genome shotgun sequence.
ACCESSION   DASRJB010000858 DASRJB010000000
VERSION     DASRJB010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3918619"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..764)
                     /gene="kdpC"
                     /locus_tag="VFB80_22550"
     CDS             complement(135..764)
                     /gene="kdpC"
                     /locus_tag="VFB80_22550"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZN36635.1"
                     /translation="MTTDLLRQFRPAAVLFLLLTLLTGVIYPLAVTGVAAVLFPGPAA
                     GSLIVRNGKTVGSRLIGQPFTGDTYFWSRPSATGPYPYNAGSSSGSNLGPLNPELADA
                     ALQRTAKLRTGHPHQPGRVPVDLVTASASGLDPHISPAAAYYQVERVAAARGLPVQQV
                     RRLVEEHIEPRTLGILGEPRVNVLLLNLALKENSKIQRTKEPKEDRRGF"
     gene            complement(779..>2225)
                     /gene="kdpB"
                     /locus_tag="VFB80_22555"
     CDS             complement(779..>2225)
                     /gene="kdpB"
                     /locus_tag="VFB80_22555"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZN36636.1"
                     /translation="KTPNEIALSILLSGFTIIFLLVCATLLPYSLFSVGEAKRLDPGS
                     LAAPITVTVLIALLVCLIPTTIGGLLSAIGIAGMDRMIQANVIAMSGRAVEAAGDVNT
                     LLLDKTGTITLGNRQAVEFLPAEGVAAEKLADAAQLASLADETPEGRSIVVLAKEKYG
                     LRGRDVQQLHATFIPFTAQTRMSGVNLDGRRIRKGALDAVSAHVSQAGGKLTERVLRE
                     ADRVARAGGTPLVVAEGNQALGVIHLKDIVKGGIKERFAELRQMGIKTVMITGDNPLT
                     AAAIAAEAGVDDFLAEATPEAKLKLIREQQAGGRLVAMTGDGTNDAPALAQADVAVAM
                     NSGTQAAKEAGNMVDLDSNPTKLIEVVRIGKQLLITRGALTTFSIANDVAKYFAIIPA
                     AFASTYPVLNKLNVMHLATPSSAILAAVIFNALIIVFLIPLALRGVKYRAASAATLLR
                     DNLLVYGAGGLIVPFIGIKLIDIALVALRLA"
BASE COUNT          365 a          675 c          802 g          383 t
ORIGIN      
        1 ccggcgcagc gccgaagaag agcttcagct tgccgcggcg gcgcttgacc tctgcggcct
       61 gcacctgcgc caagagcgaa tcgggatcgg ggcgactgga catcaaaaaa ttaaagaacc
      121 gaagaaccga cgaatcaaaa accgcgcctg tcctcttttg gttctttggt tctttggatc
      181 ttggagtttt ccttcagcgc caaattcagc agcagcacat tgactcgcgg ctcgcccaga
      241 attcccaggg tccgcggctc gatgtgctct tccaccagcc gccgcacctg ttggacgggg
      301 agtccgcggg cggcggcgac ccgctctact tgataatacg cggccgcggg cgagatgtgc
      361 ggatcgaggc cgctggccga ggcggtgacc aggtccaccg gaacgcggcc gggctgatgg
      421 ggatggccgg ttcgcagctt tgcggtgcgc tgcagggcgg cgtcagccag ctccggattg
      481 agcgggccga ggttcgaccc gctcgaagag cccgcgttgt agggataggg gccggtcgcc
      541 gacggccgac tccagaaata cgtgtcgccc gtgaacggct gcccgatcag ccggctgccg
      601 acggttttgc cattccgcac gatcaggctg ccggcagcgg gaccgggaaa cagcaccgcc
      661 gcgacgccgg taacggcgag cggatagatc acgccggtga ggagcgtgag caggaggaac
      721 aggaccgcgg cggggcggaa ttggcggagg aggtcggtgg tcatggtctt cttgaggttc
      781 aggccagtct cagggcgacg agcgcgatat cgatcaactt gatgccgatg aacgggacga
      841 tcaggccgcc cgcgccgtag acgagcaggt tgtcgcggag cagcgtggcg gcactggcgg
      901 cgcggtattt cacgccgcgg agggcgagcg gaatgaggaa cacgatgatc agggcgttga
      961 agatcacggc cgcgaggatg gcactgctcg gggttgccag gtgcatcacg ttcagcttgt
     1021 tcaagacggg ataggtgctg gcgaacgcgg cgggaatgat cgcgaagtac ttggccacgt
     1081 cgttggcgat gctgaacgtg gtcagcgcgc cgcgggtgat gagcagctgt ttgccgatcc
     1141 gcacgacctc gatcagcttc gtggggttgc tgtcgagatc gaccatgttg ccggcctcct
     1201 tggcggcctg cgtgccgctg ttcatggcga cggccacgtc ggcctgcgcc agcgccggcg
     1261 cgtcgttcgt gccgtcgccg gtcatcgcca cgaggcggcc gccggcctgc tgctcgcgga
     1321 tgagcttcag cttggcctcg ggcgtggctt cggccaggaa gtcatcgacg ccggcctcgg
     1381 ctgcgatcgc ggccgcggtg agagggttgt cgcccgtaat catgaccgtc ttgataccca
     1441 tctggcgcag ctcggcgaac cgctccttga tcccgccttt gacgatgtcc ttgaggtgga
     1501 tcacgcccag ggcctggttg ccttcggcga cgaccagcgg cgtgccgccc gcgcgggcca
     1561 cgcggtcggc ctcgcgcagc acccgctcgg tcagcttgcc gccggcctgg ctgacgtggg
     1621 cggaaacagc gtccagagcg cctttgcgaa ttcgccggcc gtcgaggttc acgccgctca
     1681 tgcgggtctg ggcggtgaag ggaatgaacg tggcatggag ctgctgcacg tcgcggccgc
     1741 ggaggccgta tttctccttg gcgagcacga cgatgctgcg gccttcgggc gtctcgtcgg
     1801 ccagcgaggc gagctgggcc gcatcggcca gcttctcggc ggcgacgcct tcggctggca
     1861 ggaactcgac ggcctgccgg ttgccgagcg tgatcgtgcc ggtcttgtcg agcagcagcg
     1921 tgttgacgtc gccggcggct tccacggccc gccccgacat ggcgatgacg ttggcctgaa
     1981 tcatgcggtc catgccggcg atgccgatcg ccgaaaggag gccgccgatc gtcgtgggaa
     2041 tcaggcagac caagagcgcg atcaagacgg tgacggtgat cggtgcggcc agggaacctg
     2101 gatcgagcct cttcgcctcg ccaacgctga acagcgaata gggcagcagc gtggcgcaga
     2161 cgagcaggaa gatgatcgtg aatccggaca gcaggatgct gagggcgatt tcgttcgggg
     2221 tcttc
//
LOCUS       DASRJB010000859         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1107331, whole genome shotgun sequence.
ACCESSION   DASRJB010000859 DASRJB010000000
VERSION     DASRJB010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107331"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFB80_22560"
     CDS             complement(<1..796)
                     /locus_tag="VFB80_22560"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZN36637.1"
                     /translation="MKRSLFQAVTALLSVLVLISNAAAQDAKTTDAAKSAEPAKSSET
                     SKQVVAVFRLKGGLTETPTDESFPFALEQTTALKSLVQRMQKAAADKDVRAVVITLSD
                     FGAGFAQIEELRQAIAGLRAAGKDVYAHADSLSMPQYLLLSGATQLSVVPTGDLWITG
                     LAGESLHVRKLLDLIGAKPDFLTCGAYKSAAEMFMRPSPSKEADEMNNWLLDSLYESS
                     LKLIAGGRGVTPEKVRQWIDEGPYTATKAKEAGLIDAVQHRQDFEAD"
     gene            complement(892..2511)
                     /locus_tag="VFB80_22565"
     CDS             complement(892..2511)
                     /locus_tag="VFB80_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36638.1"
                     /translation="MTSPGQAILWQIYWRARWGLAAAAAFLLLAVALTHLLPRHWTIQ
                     MDEMDVSAVGWFFGVSCLFANFIVMAAFSMSSADARNLTFTKHMFVLPVRTGTLVAWP
                     MFSGCLTVAVIWLINACFVFRASGIAAPLWFPAAAFALLLASFQALAWTPFAQRWLHI
                     ALTTVVLTSPILVLLVGLILNLQLSEPMATCLLLGLIPIAYVAAVSGVGRARRGEAYD
                     WRAWGRFVESLAKWRPAVTHPFRSIGTAQLWYECRAHLIVPVFIACLMPCLIFVPALA
                     REDVALGWRLLAGLLFVPLMVAGLAGGALGNLTDPISKSYTASFVLVRPISSLSILRV
                     KLATAAIMTGAIWLLFLGYISLLLTRPGFPQSIAQVASSAGVWKAIGYPILALSLLVL
                     LTWKNMVASLWVTLTGRKWVEQANSFGFIGLVFIGIGIGLWVGFHPELHQPALAAVPW
                     LIGLLLVMKLAAAGLVVRGLVQSRLTGPAGAALMVAMWLAAVGSLCALALFLLPPEYA
                     APKNVIPGIALFSPFSRLAGAPLALEWNRHR"
     gene            complement(2516..3442)
                     /locus_tag="VFB80_22570"
     CDS             complement(2516..3442)
                     /locus_tag="VFB80_22570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN36639.1"
                     /translation="MRSPEFLFSDDVIEICGVTRKFGAKRALDNVSLAIPRGVVFGLV
                     GANGAGKTTLIRHLLGLLQAQTGSVRVFGLDPVQEPVRVLARVGYLSEEGDLPGWMRV
                     DELMRYMRAFYPTWDEVYAQDLRRQFELDPAAKVKTLSKGQRARAGLLVALAYRPELL
                     LLDEPSSGLDPIVRRDILGAIVRTIADEGRTVLFSSHLLDEVERISDRVAMLKGGRIL
                     FTGELDEIKQTHHRLTLRFLDERRQPPELNGVLSWEGRGYIWTAVAHGRLDDLALAAS
                     AAGAEIIDQRALSLDEIFLAQVGTPRLQPEEV"
     gene            complement(3444..3857)
                     /locus_tag="VFB80_22575"
     CDS             complement(3444..3857)
                     /locus_tag="VFB80_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN36640.1"
                     /translation="MQIHISSHDGVPIYLQIVNQVKYLVASGRLAAGEELPPIRTLAE
                     QLLVNPNTVARAYRELELAGVVTKRRTAGTYVSDGASPLARRERLKIVTQRIDALLAE
                     ARQMNVDTATLIELIQERDEAMQPLEAAAPRTKEK"
     gene            4027..>4734
                     /locus_tag="VFB80_22580"
     CDS             4027..>4734
                     /locus_tag="VFB80_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36641.1"
                     /translation="MSKIRAMANPLANSLLVLVLAAAAGLSSAQEPAAATPQETYQQW
                     LAERTKEFQSYDFSREGAPPKTLTMEARSLLNWSNAERNTAAGAVFLWTHEGRPELIA
                     CAFGRGQWLRHEFHSLSETAIAGEREGQRVHRFKPGIEWRELPGAPEPAAARPLRLTQ
                     MRRQAERFRVSIVALKMGVPEPAVMRLLSQPVYRSPETAMDDTALFLFVQGTDPECVL
                     TLQATAGKTWRYALTRQT"
BASE COUNT          892 a         1543 c         1538 g          761 t
ORIGIN      
        1 cgtccgcctc aaaatcctgc cggtgctgca cggcgtcgat caagccggcc tctttggcct
       61 tggtcgccgt gtacggccct tcgtcgatcc actggcgcac cttttccggc gtaacgccgc
      121 ggccgccggc gatcagcttc aagctgcttt catagaggct gtccagcagc cagttattca
      181 tctcatccgc ttccttgctc ggcgaagggc gcatgaacat ttcggccgcg ctcttgtagg
      241 ccccgcaggt caggaagtcg ggcttggcgc cgatcaggtc cagcagcttg cggacgtgca
      301 ggctctcgcc ggcgagcccc gtgatccaca ggtcgcccgt cggcacgacg ctgagctgcg
      361 tggccccgct caagagcagg tactgcggca tcgacagcga atcggcatgg gcatagacgt
      421 ccttgcccgc cgcccgcagt ccggcgatcg cctgccgcag ctcttcgatc tgcgcgaagc
      481 ctgccccgaa atcgctgagc gtgatcacga cggccctaac gtccttgtcc gccgccgctt
      541 tctgcattcg ctgcaccagg ctcttgagcg cggtcgtttg ctccagcgcg aacgggaagc
      601 tctcgtcggt cggcgtttcg gtcagcccgc ccttcaatcg gaatacggcg acgacctgtt
      661 tcgacgtctc actcgatttg gccggctctg cgcttttggc cgcgtcggtt gtcttggcat
      721 cctgcgccgc cgcgttggat atgagcacca gcacggacag cagcgcggtc actgcttgga
      781 aaagactgcg tttcatagcg aatgctcccc tcgttggtga aaagcgttct caataccgag
      841 tacccagtac ttcgtacaga tagttttggg cttgcggcca gaaccggata attatcggtg
      901 ccggttccac tccaaagcca gcggtgcgcc ggcgaggcgg ctaaaggggc tgaacagggc
      961 gatgccagga atgacgttct tgggcgcagc atattcgggt ggcaacagaa acagcgccag
     1021 cgcgcacagc gatcccacgg cggcgagcca catcgccacc atcagcgccg cgccggcggg
     1081 accagtcagg cgcgactgaa caagtccccg cacaaccagg cccgcggctg cgagcttcat
     1141 cacgagcaac aagccaatca gccagggcac cgcggcgagc gccggttggt gcaactccgg
     1201 atggaagccg acccataagc cgatgccgat tccgatgaac accagcccga tgaaaccaaa
     1261 gctgttcgcc tgctccaccc acttccggcc cgtcagagtg acccataggc tggcgaccat
     1321 gttcttccag gtaaggagca cgagcaggga gagcgccaag attggatagc cgatggcttt
     1381 ccacacgccg gcgctgctcg cgacctgggc aatggactgg ggaaaaccgg ggcgcgtcag
     1441 caagagcgag atatagccca gaaagagcag ccagatcgcc ccggtcatga tcgcggccgt
     1501 cgccagcttt actcgcaaaa tcgacaggct ggaaatcggc cgaaccagaa cgaatgacgc
     1561 ggtgtacgac ttggagattg gatcggtaag gtttccgagc gctccgccgg cgagcccggc
     1621 gaccatcagt ggaacaaaaa gcaagcccgc cagcagccgc cagcctaggg cgacatcttc
     1681 gcgcgctagt gccggcacga agattaagca gggcatcagg caagcaatga acaccggcac
     1741 gatcaggtgg gcgcggcact cgtaccagag ctgggccgtg ccaatcgagc ggaaggggtg
     1801 agtcaccgcc ggtcgccact tagccagcga ttcgacaaag cggccccagg cccgccaatc
     1861 atacgcctcg ccgcgccgcg ctcggccgac gcctgagacc gcggccacat acgcgatggg
     1921 tatcaagccg agcaacaggc acgtggccat cggttcgctg agctgaaggt tgagaatcag
     1981 ccccaccagc aagaccagaa tcggcgaggt cagtacgact gtagtcaagg cgatatgcag
     2041 ccaccgctgt gcaaatggcg tccacgcgag cgcttgaaac gaggccaaca aaagagcgaa
     2101 ggcggccgcc ggaaaccaaa gcggcgcagc gattccgctg gcacgaaaca cgaagcacgc
     2161 gttgatcagc cagatgaccg cgacggtcaa gcagccgctg aacatcggcc aggccaccag
     2221 cgtgccggtg cggactggca gcacaaacat gtgtttggtg aacgtcaggt tgcgagcgtc
     2281 cgcactgctc atgctaaagg ctgccatcac gatgaagttg gcaaacaggc acgagacgcc
     2341 gaagaaccag ccgacggccg aaacgtccat ttcgtccatc tggatcgtcc agtgtctcgg
     2401 cagcaggtgg gtgagcgcta ccgccagcag caggaacgcg gcggccgccg ccaagcccca
     2461 gcgggcccgc cagtaaatct gccacaagat ggcctgtccc ggtgaagtca tgaggctaga
     2521 cctcctccgg ctgcagtcgc ggcgtgccga cctgggccag gaaaatctcg tcgagcgaaa
     2581 gcgctcgctg gtcgatgatc tcggcacccg cggcagaggc ggcgagcgct agatcgtcga
     2641 gccggccatg ggccacggcg gtccagatgt agccgcggcc ctcccacgag aggacgccgt
     2701 tcagctccgg cggctgccgc cgctcgtcca ggaaacggag cgtcaggcgg tggtgcgttt
     2761 gcttgatttc gtcgagctcg ccggtgaaga gaatgcggcc gcccttgagc atggccacgc
     2821 ggtcggagat ccgctcgacc tcgtcgagca agtgcgacga gaagagcacc gtgcggcctt
     2881 cgtcggcgat ggtgcggacg atcgcaccca ggatgtcgcg gcggacgatc ggatcgaggc
     2941 cgctcgaagg ctcgtcgagc agcagcagct ccgggcgata ggccagcgcg acgagcaggc
     3001 cggcccgcgc cctttgtcct ttggagagcg tttttacctt ggccgccgga tcgagctcga
     3061 actgccgccg caagtcctgc gcgtaaactt cgtcccaagt tgggtagaag gcccgcatgt
     3121 accgcatcaa ttcatcgacc cgcatccagc ccggcaagtc gccttcttcg gagaggtagc
     3181 cgacgcgcgc gagcacgcga accggctcct ggaccggatc gaggccgaac acgcggaccg
     3241 agccggtctg ggcctggagc agccccagca ggtggcggat cagcgtcgtc ttgcccgcgc
     3301 cgttggcccc caccaggcca aacaccacgc cccgcgggat ggccaggctg acgttgtcca
     3361 gcgcccgctt cgcgccgaac tttcgcgtca cgccgcaaat ctcgatcacg tcgtcggaga
     3421 acaggaattc gggggaccgc atgtcatttc tccttggtgc gcggcgccgc ggcctccagc
     3481 ggctgcatgg cttcgtcgcg ctcctgaatg agctcgatga gggtggcggt atcgacgttc
     3541 atctgccggg cctcggccag cagggcgtcg atccgctggg tgacgatctt cagccgctcg
     3601 cggcgggcca gcggcgaggc gccgtcggag acgtacgtgc cggcggtgcg gcgcttggtc
     3661 acgacgccgg ccagctccag ctcgcggtag gcgcgggcca ccgtgttggg gttcacgagc
     3721 agctgctcgg ccagcgtgcg gatcggcggc agctcctcgc cggccgcgag gcggcccgag
     3781 gccaccaggt acttcacctg attgacgatc tgcaaataga tcggcacgcc gtcgtgggag
     3841 gagatgtgga tttgcacggc actgcgttct ggagtctcgg atcgcttgta ctaattcagt
     3901 aatacaaccg cgtgcggttg tcaatacccg agagcaaact gctcccggtg tccgccagtg
     3961 aggccaccac ggcgaacgtc cgggaccagg tttggtcccg gctaagaatt gcgtaagtca
     4021 gacaccatgt ctaagatacg cgccatggcg aatcccttgg ccaactcgct gctcgtgctg
     4081 gtgctggccg cggctgctgg gctttcgtcg gcccaggagc cggcggccgc gacgccccag
     4141 gagacctacc agcagtggct cgctgagcgc acgaaggaat tccagtcgta cgacttttcc
     4201 cgggaaggag cgccgccgaa gactttgacg atggaggccc gctcgctcct caactggtcg
     4261 aacgccgaac ggaatacggc tgccggggca gtgttcctgt ggacgcacga aggacgaccg
     4321 gagctgatcg cctgcgcgtt cggccgggga cagtggctgc ggcacgagtt ccattcgctg
     4381 agcgaaacgg cgatcgccgg cgagcgggaa ggccagcgcg tgcaccgatt caagcccggc
     4441 atcgaatggc gcgaattgcc cggcgctccc gagcccgcgg ctgcgcggcc cctgcggctg
     4501 acccagatgc ggcggcaggc ggagcggttc cgggtctcga tcgtcgcgct gaagatgggc
     4561 gtaccggagc ccgccgtcat gcggctgctg agccagccgg tctatcgctc gcccgaaacg
     4621 gcgatggacg ataccgccct gttcctgttc gtgcagggga ccgacccgga gtgcgtgctg
     4681 acgcttcagg cgacggccgg caagacgtgg cggtacgccc tgacccgcca gacg
//
LOCUS       DASRJB010000860         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3070037, whole genome shotgun sequence.
ACCESSION   DASRJB010000860 DASRJB010000000
VERSION     DASRJB010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070037"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(686..1243)
                     /locus_tag="VFB80_22585"
     CDS             complement(686..1243)
                     /locus_tag="VFB80_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN36642.1"
                     /translation="MSDVTELLDAAAGGDRRAAAELLPLVYGELRQLAAARMAAESPG
                     NTLQPTALVHEAYLRLVGPVAGDRWDSRGHFFAAAAEAMRRILVESARRKESLKHGGG
                     RQRADYDVVDSASVERPEELLAVDEALAGLAAVDEQAAELVKLRYFAGMSMPEAAKVM
                     NLPLRSAERLWTFARAWLRRDIERE"
     gene            complement(1430..1504)
                     /locus_tag="VFB80_22590"
     tRNA            complement(1430..1504)
                     /locus_tag="VFB80_22590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1467..1469),aa:Pro,seq:cgg)
     gene            1672..2517
                     /gene="arsM"
                     /locus_tag="VFB80_22595"
     CDS             1672..2517
                     /gene="arsM"
                     /locus_tag="VFB80_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HZN36643.1"
                     /translation="MPESPSDAIREQYGAAAQRGLSSEQPAVAAVAAAFGYSADELQS
                     IPAAANLGLSCGNPTVFAGLRLGEVVVDLGCGGGLDVFLAARKVGPQGRAIGIDMTDE
                     MLALARRNAARGPDGQPYSNVEFHRATIDRLPLASGSVDCVISNCVINLAPDKAAVFR
                     EIARVLKAGGRLAVSDIALKRPLPAELAGDLAALVGCIAGALPIAEYQSGLAAAGFAQ
                     VEVVDTRADLNAYALIDDQAACCSPGQGCGPADAGLHARLADLLRRYDVNDYAASVRV
                     YALKA"
     gene            2562..>3148
                     /locus_tag="VFB80_22600"
     CDS             2562..>3148
                     /locus_tag="VFB80_22600"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN36644.1"
                     /translation="MPLDPAADADWAPLKKHWILRRDTTYLNHGSFGPPPAAVRERRE
                     HWQHQLDTQPMDFFVREFEPAWLAARGALAKFVGAEPGNLIFAENATVAMNIVADSFP
                     LAAGDEVLLTDHEYGAVQRIWQRACEKSGAAAKTVELPLPVQSSAQLLEVIFSAASQH
                     TRLIVVSHITSPTAIILPVEEICREAQRRGIAVAI"
BASE COUNT          571 a         1027 c         1022 g          528 t
ORIGIN      
        1 acgccgcgct tgccgtttgg atattctccc ggtgtttttt gaaacgccac cgttcatcgc
       61 ccaaccaaac agggaacgta ctgagcgcat ggaactctcc aatcgaatga gacccgcggg
      121 gaagacgaag acatctcacg agccgcgggc caaagattcg tgagaggtgc gatcagaaaa
      181 tgcccgcgaa gccgcggcgc gatgaccagt agttgcggat gacctggctt gtgggaccag
      241 aacaggtgcg gtcgcgagaa ccaacgcagc gtttctatcc tgctaagtcg cagtttctcg
      301 caatccgctg ggcgttaagc gcgcaaaatg acgccggcgc gctagagttc gttgccaaag
      361 ggcccaggtt attccggtcc ggccggtcaa cacgcaaacg ctgcaagcgc tactaacagg
      421 attcgcagtc gatcactagc gtgctgtggc aaagcaccat agtcctcgcc gcccgtggga
      481 tattcatctc gcttgagcaa ctttgcgatg aatcgaaaca tgacttgttc ctcgctgaaa
      541 ggggacgttg ccaagccgct cgaaccattt cgcatcattc ctacgcagtt gagtttggtt
      601 cgaatcggcg tatcgcaatc caatgcgaca gcggcaggaa aaccccgcca agatttttag
      661 aatgggagcg cgggcgatct aggctttact cgcgctcaat gtcccgtcgc agccaggccc
      721 gggcgaacgt ccagagccgt tcggcagagc gtagcggcaa gttcatcact ttcgcggctt
      781 cgggcatgct catgcccgca aaatagcgca gcttgaccag ctcggcagcc tgctcgtcaa
      841 cggcggccag gcctgctagt gcctcgtcca cggccagcaa ctcctcaggc cgctcgacgc
      901 tcgctgagtc cacgacgtca tagtccgcac gttgccgccc accaccatgt ttgagcgact
      961 ccttgcggcg ggccgactcc acgaggatcc gccgcatcgc ctccgccgca gccgcaaaaa
     1021 agtgcccgcg agagtcccaa cgatcccccg ccaccggccc taccagccgc aagtaagcct
     1081 cgtgaaccag cgctgtgggc tgcagcgtgt taccgggcga ttcggccgcc atccgcgcgg
     1141 cagcgagctg ccgcagctca ccgtaaacaa gtggcaatag ctccgcggcc gcacggcggt
     1201 cgccgcccgc ggcggcatca agcaactcgg tgacatccga catgccaacc agtttagggc
     1261 cggccttggg ccgcacaagc caaagccagc gagctgacga tcgcttattt agagcctaag
     1321 aggtcgcagc aaagagtgcg cgttttccgc gcgcgccgcg acagaagata gcggctgttc
     1381 agctagagtt gcaccaatgg ccggggggtg cgaatcgtcg taagtctagt cggggtgaca
     1441 ggatttgaac ctgcgacctc ttggtcccga accaagcgct ctagccaggc tgagccacac
     1501 cccgggggga ggggcggtgg gcccctcgac ggaaagctta tcgtaacgga acgggcatcg
     1561 cggtcaacgg cagcatactg gtgaggacgt gcgggggcgg cggaggttcg tgccgcccgc
     1621 gcgtcgcctg ttagaatttg ccgccagcgg cccctcttta cggtgcgacc gatgcctgaa
     1681 tcgccgagcg acgccattcg ggagcaatac ggagctgccg cccagcgcgg cttgtcgagc
     1741 gagcagccgg ccgtagcggc cgtggcggct gccttcggct attcggcgga tgaattgcag
     1801 tcgattccgg cggcagcgaa ccttgggctt tcgtgcggca acccgaccgt gtttgctgga
     1861 ctgcggctgg gcgaagtcgt ggtggatctg gggtgcggcg gcgggctcga cgtgttcctg
     1921 gcggctcgca aggtcgggcc gcaagggcgt gcgatcggca tcgacatgac ggacgagatg
     1981 ctcgcgctgg cccgccgcaa tgccgcccgc gggccggacg gccagcccta ttcgaacgtc
     2041 gaatttcacc gcgcgacgat cgaccgcctg ccgctggcga gcggctcggt cgattgcgtg
     2101 atcagcaatt gcgtcatcaa tctcgcgccg gacaaggcgg ccgtctttcg cgagatcgcc
     2161 cgcgtgctca aggccggtgg ccggctggcg gtgagcgaca tcgccctcaa gcgcccgctc
     2221 cccgcggaat tggcaggcga tctggcggcg ctcgtcggct gcatcgccgg ggcgctgccg
     2281 atcgcggaat accagagcgg actggcggcc gccggctttg cccaggtcga agtcgtcgat
     2341 acgcgggccg acctcaacgc ctacgcgctg atcgacgacc aggcggcctg ctgttcgccc
     2401 gggcaaggct gtggcccggc ggatgccggc ctgcacgcgc ggctggccga cctgctgcgg
     2461 cgctacgacg tgaacgacta cgccgccagc gtccgcgtgt atgcgctgaa ggcgtagccc
     2521 ggttatgatc ggtgctcccc gccttccgcc gcctgcgaac catgccactt gatcccgccg
     2581 ccgacgccga ctgggcgccg ctcaagaaac actggattct gcggcgcgac acgacgtacc
     2641 tcaatcacgg ctcgttcggg ccgccgccag cagcggtccg cgagcggcgc gagcactggc
     2701 agcaccagct cgacacgcag ccgatggatt tcttcgtccg cgagttcgag ccggcgtggc
     2761 tggcggcgcg gggcgctctg gcaaaattcg tcggcgcaga gccgggcaac ctgatctttg
     2821 ccgagaacgc cacggtggcg atgaacatcg tcgcggactc ctttccgctg gcggccggcg
     2881 acgaggtgct gctcaccgat cacgaatacg gcgcggtgca gcggatctgg cagcgagctt
     2941 gtgaaaaaag cggggcagcg gccaaaaccg tggagctgcc gctgccggtg cagtcgtccg
     3001 cgcagctgct ggaggtgatt ttctcggcag cgagccagca cacgcggctg atcgtcgtca
     3061 gccacatcac cagcccgacc gccatcatcc tgccggtcga ggaaatctgc cgcgaggcgc
     3121 agcggcgggg catcgccgtc gccatcga
//
LOCUS       DASRJB010000861         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_545240, whole genome shotgun sequence.
ACCESSION   DASRJB010000861 DASRJB010000000
VERSION     DASRJB010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545240"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1366
                     /locus_tag="VFB80_22605"
     CDS             182..1366
                     /locus_tag="VFB80_22605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZN36645.1"
                     /translation="MSYIVSARRTPIGKLLGSLASVPAPQLAAVAIRAALADSRLMEK
                     VVDQVILGNVLSAGVGQAPARQAALAAGLPPTVGALTINKVCGSGLAAVMLADQAIRA
                     GDAHVVIAGGMENMSLAPHLLVGARSGWKFGHQQTLDSMLHDGLWCARENQGMGSLSD
                     YTATKCGVSREDQDAFSVESHRRAVAAGEASKFTAEIAPVAVKAGKSEVLVSRDEGPR
                     ADCTLADLAKLRPAFGEGGTATAGNASQISDGAAALVVASDSAAQPLKSTFRARIVAT
                     AVNSGEPRELFTAPIGAIERVLAKADLQVRDIDLFELNEAFAAQSLACARPLGIDPAK
                     LNVHGGAIALGHPIGASGARVLVTLLHALQDRGLRYGLAALCLGGGEAVALIVDCQEP
                     AR"
     gene            1419..>2272
                     /locus_tag="VFB80_22610"
     CDS             1419..>2272
                     /locus_tag="VFB80_22610"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZN36646.1"
                     /translation="MLRCLVVVFLFAPCIAYGQERAAAPILIDTDIGGAIDDAFALGL
                     TLSSPEVELLGVTTVGEAAEDRAWIVCRLLTHAGRTNVPVAFGRGEQPKGPIDWQIQY
                     RRHPAVVWSRTNKPAATPAVELLYEKLKAAPREVTVVALGPLTNVAQLFREHPDAKSL
                     LKRLVVMGGALEVGYNAQPPAVSEWNIKQDVAAAQAVFAAGVPTLVVPLDATAGVKFA
                     PEQERRLFAAGTPLAFQLANLMELCEQPDQSLFDAVAVAAATSDQFLTIEDRRLKVAD
                     DGRTLAGP"
BASE COUNT          349 a          758 c          792 g          373 t
ORIGIN      
        1 gcccgaaatc ggccagcagc cgagggccac gggcgaaacc tgcaggcccg tgttgccgag
       61 cagacgcagc tcgatgggca gcatgtttcc tccgggttgg tgtgagccgg caaaggcgag
      121 ttgcgtagaa ttgtaatgcg accgaatgtg cttggtgtct tgtaattgga tcctggctcc
      181 tatgtcctac atcgtttctg cccgccgcac gccgattggc aagctgcttg gctcgctggc
      241 gagcgtgccc gcgccgcaac tcgccgccgt tgccattcgg gccgcactgg ccgattcccg
      301 gctgatggag aaggtcgtcg atcaagtcat tctgggcaac gtgctctccg cgggcgtggg
      361 gcaagcgccg gcgcggcagg cggcgctcgc cgcgggattg ccgcccacgg tcggggcgct
      421 gacgatcaac aaggtctgcg gcagcggcct cgccgcggtg atgctggccg accaggcgat
      481 tcgcgccggt gacgcgcacg tcgtgattgc cgggggcatg gagaacatga gcctcgcgcc
      541 gcacctcctg gtcggtgctc gcagcggctg gaagttcggc catcagcaga cgctcgattc
      601 gatgctgcac gacgggctgt ggtgcgcccg cgagaaccag ggcatggggt cgctgtccga
      661 ctatacggcc acgaagtgcg gcgtgagccg cgaggatcag gacgcgtttt cagtcgagag
      721 ccatcggcgg gccgtcgccg ccggtgaggc gagcaagttc acggcggaaa tcgccccggt
      781 cgcggtcaag gctggcaagt cggaagtgct ggtcagccgg gacgaagggc cgcgggccga
      841 ctgcacgctc gccgatctgg ccaaattgcg gcccgccttc ggcgagggtg gcacggcgac
      901 tgccggcaac gcctcgcaaa tcagcgacgg ggccgcggcg ctggtcgtcg ccagtgattc
      961 ggccgcccag ccgctcaaat cgacgtttcg ggccaggatc gtcgccaccg ccgtgaattc
     1021 gggcgagccg cgcgagctgt tcaccgcgcc gatcggcgcg atcgagcgcg tgctggccaa
     1081 ggccgatctg caggtccgcg acatcgacct cttcgagttg aacgaagcct ttgccgcgca
     1141 gagcctggcc tgcgctcgcc cgctgggcat cgacccggcg aagctcaacg tgcatggcgg
     1201 cgccatcgcc ctgggccatc cgatcggcgc gagcggcgcg cgggtgctgg tcacgctgct
     1261 gcacgcgctg caagaccgcg gtctgcgata cggcctcgcc gctctctgcc tcggcggcgg
     1321 cgaggcggtc gccctgatcg tggattgcca agagccggcc cggtaatcct tgcttcgcgc
     1381 agtggcgaag gtgtatcgtg cgcgctggag gcctactcat gctgcgctgc cttgtggttg
     1441 tcttcctctt tgctccctgc atcgcgtatg gacaggaaag agcggcagcg ccgatcctga
     1501 tcgacaccga catcggcggc gcgatcgacg acgcctttgc cctgggcctg acgctgagca
     1561 gtcccgaggt ggagctgcta ggcgttacca cggtcggcga agcggccgag gaccgggcct
     1621 ggatcgtttg ccgcctgctg acgcacgccg gccgcaccaa tgtgcccgtg gccttcggcc
     1681 gcggcgagca gccgaagggg ccaatcgact ggcagattca gtatcgccgt catccggccg
     1741 tcgtttggag ccgcaccaac aaacccgcgg ccacgccggc ggtcgagttg ctctacgaga
     1801 agctgaaggc cgccccgcgc gaagtcaccg tcgtggccct gggcccgctg accaacgtcg
     1861 cgcagctctt ccgcgagcat cccgatgcga agtcgctgct caagcgtctg gtcgtcatgg
     1921 gcggcgcgct cgaggtcggc tacaacgccc agccgcccgc cgtcagcgaa tggaacatca
     1981 agcaggacgt cgccgcggct caggcggtat tcgcggccgg cgtgcccacg ctcgttgttc
     2041 cgctggatgc aaccgcgggc gtcaaattcg cgccggagca ggaacgacgc ttgtttgcag
     2101 ccggcactcc gctggcgttt caactggcaa acctgatgga gctctgcgag cagccggacc
     2161 agtcgctgtt cgacgcggtg gccgtcgcgg ctgcgacgag cgaccagttc ctcacgatcg
     2221 aagaccgccg cctgaaggtc gccgacgacg gccgcacgct cgctggcccg ga
//
LOCUS       DASRJB010000862         6458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3070491, whole genome shotgun sequence.
ACCESSION   DASRJB010000862 DASRJB010000000
VERSION     DASRJB010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070491"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /locus_tag="VFB80_22615"
     CDS             complement(<1..2597)
                     /locus_tag="VFB80_22615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36647.1"
                     /translation="MPDHFDPYYTWLGIPPDEQPADHYRLLGVRPFESNDEVIVNAGD
                     QRMAYLRTLQVGKRSKESQALLNEISAAQGCLLDPKRKQAYDAELRRKRGPQRPAADS
                     TPVVPVEARRQARPIAAPLHATPAPVTPLQPVIVQPAQPAQPVVISPEPRPLVDTSAG
                     PVVTRRPASVPKSSSPISLPILIGVGAAAAGLVVFLVVVVWAATRPAPRPVFVSPAPR
                     TNSPAPSPAPAPSPPQITKTPPETPSPSPSPAPAPNVAAGTGLPLGPEVILNTLPLSA
                     PSDLRIALHPKGPAVYQFAPAASELTRVDFSRASPPSKITAVHGFALSDDGSRVAMYS
                     DNRLHVRAPDNKMALVARATAGGRVKSIALNADKRLLAAAVDGDQPLLVVELASGNSR
                     PEFTTKLTSVDWVGIQGDHLCCWSPSGEGEIISLRDPSQRRPLADRTEFAPAGFTAQF
                     GLMTFNRVHPAHVSFFYVRPETGQRWAEFHVDEFADRAQLLAMSPQGDAVVVNHPGRG
                     LAVVQLPSKRVLTRIDWPGGQFHAAALTDKDGLLAVNGAQNRLLLLTMKKLLERPQWQ
                     MPATPMAGAREPADGNPAPPADNSAPSAGSSPMPPAQENSSATVDPKEALAALQAAGV
                     RLPSQVNAAMSVRTMGTSFGPQHAPLLASLPRLATLDLNQQQACGEVLQALPSLPALR
                     MLILDNTLVSDGDLVHLAKFPGLHRLSLVRTRLAGPGLENLRHLPELKSLSIPPELPR
                     DVVSLICLAKELDHCSVWPRGMTDADLEYVAGLQNMRTLDLPYANLQGPGLAHLQKLP
                     NLEALLVPSRSPPEVIRQTANLNLKHMTFPLGVGAADVALFARMPRLRSINAPSDFTD
                     QHLQALANFP"
     gene            2799..3809
                     /locus_tag="VFB80_22620"
     CDS             2799..3809
                     /locus_tag="VFB80_22620"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN36648.1"
                     /translation="MTGRAVSIVPLSQLLDGQEADTFALLAAKSEGTTRDGKPFWRVT
                     FRDCGRQVSFPIWHDSAHFDACRDHWQVGTCYKLRAAYGETKYGPQLEIRKIRPATPQ
                     DQADGFDESRLMPRSKYDSAQMFAELVQLAQSEIRSEPLRELTVGLLAAHRETLLPLA
                     AARRNHHAFAGGFLEHVLSVVRNAIFLADKYGRDYPEMQPPLSRDLVVAGAILHDIGK
                     VQEIAMTTLGAEYTAAGELIGHVLLGRDLVREAAQRHALDPETLLRLEHIIVSHQRLP
                     EWGAPKPPMTPEALLVHFADDIDAKFQTALVALAESSEGEFTAGRNPLGYKIFRGLQK
                     PE"
     gene            3918..4772
                     /locus_tag="VFB80_22625"
     CDS             3918..4772
                     /locus_tag="VFB80_22625"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HZN36649.1"
                     /translation="MPSPIAIRRKDAPFTPPADFWSDFVERFWERGATTLRYQAEQPL
                     MTEAEALAIFLGLAERFRAGGEADIDLYVENGLLKSDIGRLLPAAADRDIAAYQARVT
                     DQLGGRRFSLVTNSVRRESREHLKRVKAFTRGLIKLIGQDAGWLGADIFLSNTLKTAF
                     GVHRDSASNFSYIVSGRKRMLVWPCEVFACRRDLRCAGPKKNVFLDDVNCEPYRDQAI
                     VLEGGAGDMLYWPASYWHVAESTGKPSLVFNVPIYLNSAVNRMAAEYYLRKLSVVSSQ
                     LASPRTDH"
     gene            5018..5281
                     /locus_tag="VFB80_22630"
     CDS             5018..5281
                     /locus_tag="VFB80_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36650.1"
                     /translation="MVVPIGVWSIQERYRMAWFSKSRQQSRPRRRGVLTLEWILLVTV
                     IVIGIIGGLGVVRNATLGELADLAEAITHLNVKTTAEMMAEPP"
     gene            5424..>6458
                     /locus_tag="VFB80_22635"
     CDS             5424..>6458
                     /locus_tag="VFB80_22635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZN36651.1"
                     /translation="MRHLYLDYNATTPIAPSVQQTLLPFLAEHFGNPSSSHALGRAAH
                     EALEDARGHVASLLGTGPDEVVFTGGGTESNNLALKGVAFSRGTSAGGHLVITAVEHP
                     SVVQPARFLERLGYDVTVVPVTSQGVVQPEAIARALRPDTLLVSVMLANNETGAIQPL
                     KQIGDLCHAEHVPLHTDASQAVGKIRVQVDELGVDLLTIAGHKMYAPKGVGALYVRQG
                     VALEPALHGAGHEAGLRAGTENVAYIAALGAAAALAGKSLDASQERLLSLRDRLLALL
                     REGAGEKLTVHSESAPRLPNTLSISFPRATGHELLARIPELCASTGSACHSQTPAMSP
                     TLAAMGVSSEV"
BASE COUNT         1071 a         2122 c         2181 g         1084 t
ORIGIN      
        1 ggaaaattgg ccagcgcctg caggtgctgg tcggtgaagt ccgacggcgc attaatcgaa
       61 cggagccgcg gcatgcgggc gaagagggcg acgtcggccg cccccacgcc caatggaaag
      121 gtcatatgct tcaggtttag attggcggtc tgccggatca cttcgggagg cgatcgtgag
      181 ggcaccagca gggcctccag attgggcagc ttctgcaaat gggccaggcc cggtccctga
      241 agattggcgt acggcaaatc cagcgtgcgc atgttttgca ggccggcgac atactccagg
      301 tcggcgtcgg tcatgccgcg cggccagacg ctgcaatggt ccaattcctt ggccaggcag
      361 atcagcgaca cgacgtcgcg cggcagctcc ggcgggatcg acagcgattt cagctccggc
      421 agatggcgga gattctcgag tcctggtccg gccaggcggg tccgcacgag cgaaaggcga
      481 tgcaggcccg gaaactttgc caggtgcacc aggtcgccgt cgctcaccag ggtgttgtcg
      541 agaatcagca tccgcagcgc gggcaagctg ggcagggctt gcagtacttc gccgcaggcc
      601 tgctgctggt tcaggtccag ggtggctagc cgcggcaggc tggcgagcag cggcgcgtgc
      661 tgggggccga aactcgtccc catcgtccgc accgacatgg ccgcgttgac ttgcgacggc
      721 agtcgcacgc cggcggcctg cagggcggcc agggcctcct tggggtccac cgttgcggaa
      781 gaattttcct gggcgggcgg cattggactg ctgccggcgg acggcgcact gttatcggca
      841 ggaggcgctg gattgccgtc ggcgggctcg cgcgcgccgg ccatcggagt tgccggcatc
      901 tgccactgcg gccgctccag gagcttcttc atcgtcagca ggagcagccg gttctgagcc
      961 ccgttgacgg ccaacaggcc atccttatcg gtgagcgcgg cggcgtgaaa ttggccgccc
     1021 ggccagtcga tacgcgtgag cacccgtttg ctcggcagtt gcacgacggc caggccgcgg
     1081 cccggatggt tgacgacgac cgcgtccccc tggggcgaca tggccaagag ctgagcccga
     1141 tcggcgaact cgtccacgtg aaactcggcc caccgctggc ccgtttccgg ccgcacgtag
     1201 aagaagctga cgtgggcggg gtggacgcgg ttgaacgtca tcaggccgaa ctgcgccgtg
     1261 aatcccgccg gggcgaattc cgtccggtcg gccagcggcc gccgctgaga cggatcgcgc
     1321 agcgaaatga tctccccctc gccgctgggc gaccagcagc acaagtggtc cccctgaatg
     1381 ccgacccaat cgacgctggt cagtttcgtg gtgaactctg gtcggctgtt gccgctggcg
     1441 agttccacca cgagcagcgg ctggtcccca tcgaccgcgg ctgccaacaa tcgtttgtcg
     1501 gcattcaagg cgatcgactt cacgcgtccg cccgcggtcg ccctggccac gagggccatc
     1561 ttgttgtcag gcgcgcggac gtggaggcgg ttgtcgctgt acatcgcgac gcgactgccg
     1621 tcgtcggaca aggcaaagcc gtgaacggcg gtgatcttgc tgggcggact ggctcgagag
     1681 aagtccacgc gggtcaattc ggacgctgcc ggcgcgaact gataaaccgc cggccccttg
     1741 ggatgcaggg cgattcgcaa gtcgctcggc gctgacagcg gcagggtatt caggatgact
     1801 tcggggccta gcggcaggcc ggtgcccgcg gcaacgttcg gcgcgggcgc tggtgatggc
     1861 gaggggctgg gcgtctccgg tggcgttttc gtgatttgcg gtggacttgg cgcgggagcc
     1921 ggcgacggcg cgggcgagtt ggtgcgcggg gcaggagaga cgaacacggg tcgtggcgcg
     1981 gggcgcgtcg ccgcccagac caccacgacc agaaagacca ccaggcccgc cgccgcagcg
     2041 cccacgccga tcagaatggg cagcgaaatc ggcgaactgc tcttgggcac gctcgcgggc
     2101 cgccgcgtga ccaccggccc cgccgaggta tcgaccagcg gccgcggctc tgggctgata
     2161 acaactggct gagccggctg ggcgggctga acgatcacag gctgcagcgg cgtgaccggc
     2221 gcgggagtgg cgtgcagcgg cgcggcgatt ggccgggcct gtcgccgagc ttcgacaggc
     2281 acgacgggag tcgaatctgc cgccgggcgc tgcgggcccc gcttgcgccg caattcggcg
     2341 tcgtaggctt gcttcctttt ggggtcgagc aggcagccct gggcggccga gatttcgttg
     2401 agcagcgcct gcgactcttt ggaccgtttg ccgacctgta gcgtgcggag gtaggccatc
     2461 cgctgatcgc cggcgttgac gatcacctcg tcgttcgact cgaacggccg cacccccagc
     2521 agccgatagt ggtcggcggg ctgctcgtcg ggcgggatgc cgagccaggt gtagtacgga
     2581 tcgaagtggt caggcatatc gacctggcac ggcccgcgga agcgagcgag tgcgaatcgt
     2641 taacgcgggc tgctcaattc caatatgacc ggacgcggct ggaaatccaa gcgattggcg
     2701 gcgtccttca ccgcctcttt acaggccggc gggcccaagg catgatcccc acaaccgatg
     2761 ctgtcactcc gcgcggtctc cctgcccaat acgacgatat gacggggcgt gccgtttcga
     2821 ttgttccgct ctcgcagctg ctggatgggc aggaggccga cacgttcgcg cttttggccg
     2881 cgaagtcgga aggcacgacg cgcgacggca agccgttctg gcgggtgacc tttcgcgatt
     2941 gcgggcggca agtctcgttt ccgatctggc acgactcggc tcatttcgac gcctgccgcg
     3001 accactggca ggtcggcacc tgctataagt tgcgggcggc ctatggcgaa acgaaatatg
     3061 gcccgcagct ggaaatccgc aaaatccgcc cggcgacgcc gcaggaccag gccgacggct
     3121 tcgacgaatc gcggctcatg ccgcggagca agtacgactc ggcccagatg ttcgccgagc
     3181 tcgtgcagct ggcccaatca gaaatccgca gcgagccgct ccgcgagttg acggtcgggc
     3241 tgctcgcggc gcatcgcgag acgctgctgc cgctggccgc cgcccggcgg aaccatcacg
     3301 ccttcgcggg cggattcctg gagcacgtgc tcagcgtcgt tcgcaatgcg atcttcctgg
     3361 ccgacaagta cggccgcgac tatcccgaaa tgcagccgcc gctgtcgcgg gacctcgtcg
     3421 tggccggggc aattctgcac gacatcggca aggtccagga gatcgcgatg acgacgctcg
     3481 gcgcggaata tacggccgcg ggcgagctga tcggccacgt gctgctgggg cgggatctgg
     3541 ttcgcgaggc ggcacagcgg cacgctctcg atccggaaac gctgctccgc ctggagcaca
     3601 tcatcgtctc gcaccagcgg ctgcccgagt ggggcgcgcc caagccgccg atgacgcccg
     3661 aggcactcct ggtgcatttt gccgacgaca tcgacgccaa gttccagacc gcgctggtcg
     3721 ccctggccga gtccagcgaa ggtgaattca ccgctggccg caacccgctg ggctacaaaa
     3781 tcttccgggg cctgcagaaa ccggaataag gaattcgaac agaaggaaac gaaggcaacg
     3841 aagcgaccca catgccctct tcgtttcctt cgttatcttc tgttcgtttg cctgccctca
     3901 gctgtcgagg agttgtcatg ccgtcgccaa tcgctattcg ccgtaaggac gccccgttca
     3961 ctccgccggc cgatttctgg tcggatttcg tggagcggtt ctgggagcgg ggcgccacca
     4021 cgttgcgtta ccaggccgag cagccgctga tgactgaggc ggaggccttg gcgatcttcc
     4081 tgggcctggc cgagcggttt cgcgcggggg gcgaggccga catcgatctg tacgtggaaa
     4141 acggcctgct caaatcggac atcggccggc tgctgcccgc ggcggccgac cgcgacattg
     4201 ccgcgtacca ggcgcgggtc actgatcagc tcggtggccg gcggttttcg ctggtcacca
     4261 acagcgtccg ccgcgaatcg cgcgaacacc tgaagcgcgt gaaggcgttc acccgcgggc
     4321 tgatcaagct gatcggccag gacgcgggct ggctcggggc cgacatcttc ctcagcaaca
     4381 ccctcaaaac cgcgttcggc gtgcaccgcg attcggccag caatttcagc tacatcgtca
     4441 gcggccgcaa acggatgctg gtctggccct gcgaggtctt tgcctgccgc cgcgacctgc
     4501 gctgcgccgg gccgaagaag aacgtattcc tcgacgacgt gaactgcgag ccctaccgcg
     4561 accaggcaat cgtgctcgag ggcggcgcgg gcgacatgct ctactggccg gcgtcttact
     4621 ggcacgtggc tgaatcgacc ggaaagccct cgctcgtctt caacgtgccg atctatttga
     4681 attcggccgt caatcgaatg gcggcggagt attacctgcg gaagttgtca gttgtcagta
     4741 gtcagttggc atcgccacgc actgaccact gactgagtaa ccgctgcgac cggtacaacc
     4801 gttcgttcac caacgcgttt ctgcgactaa tcaccgtcgc ggttcgtaaa catccgggta
     4861 tcggcggcga aacatgccgc ggctgcacct gcggcgggcc caaattcgcc gcgtcgattc
     4921 aaacctttgc cctgcaaggc gttgccgtgg catcgtacgg ctgcgggccg agctaccacc
     4981 cgtttggtac gggctgtgca ttgaccggat ggtcgatgtg gttgtgccca tcggcgtctg
     5041 gtccattcag gagcggtatc gcatggcctg gttctcgaaa tctcgacagc agtcccgccc
     5101 gcgccggcgc ggtgtcttga cgttggagtg gattctcctc gtcaccgtca tcgtgatcgg
     5161 aatcatcggc ggactgggcg tcgtgcgcaa cgccacgctc ggcgagctcg cggacctggc
     5221 cgaggcgatc acgcacctga acgtcaagac caccgccgag atgatggccg agccgccgta
     5281 atcgatcctc caaccaacca ggaacggatc ccttctcgcg ttctccagag aggcgaaccg
     5341 cccactcgcc tcgctggcac atgccgcctg ggcctcgtaa gctgtgcgaa cgagcaatcg
     5401 tccgtcggtt tcgaggcccc ggcatgcggc atctctatct cgactacaac gcgaccactc
     5461 cgatcgcgcc cagcgtgcag cagacgctgc tgcccttcct ggccgagcat tttggcaatc
     5521 cttccagcag ccacgccttg ggccgcgccg cgcacgaagc gctcgaggac gcccgcgggc
     5581 acgtggccag tctgctgggc actggccccg acgaggtcgt cttcaccggc gggggcaccg
     5641 aaagcaacaa cctggccctc aaaggggttg ccttcagccg cggcacgagc gccggcgggc
     5701 acctggtcat cacggcggtc gagcacccca gcgtggttca gccggcccgc tttctggagc
     5761 ggctgggcta cgacgtgacg gtcgtgcccg tgaccagcca gggcgtcgtg cagccggaag
     5821 cgatcgcgcg ggccctgcgt cccgacacgc tgctggtcag cgtcatgctg gccaacaacg
     5881 agaccggggc gatccagccg ctgaagcaaa tcggcgacct ctgccacgcc gagcacgtgc
     5941 cgctgcacac cgacgcgtcc caggcggtcg gcaagatccg cgtgcaagtc gatgagctgg
     6001 gtgtcgatct gctgacgatc gccggccaca agatgtatgc tcccaagggg gtcggtgcgc
     6061 tctacgtgcg gcagggggtc gccctggagc ccgccttgca cggagcgggc cacgaggcgg
     6121 gcctgcgggc cggcaccgag aatgtcgcct atatcgcggc cctcggcgca gcggccgctc
     6181 tggcgggcaa aagcctcgac gcttcgcagg aacggctcct ttccctgcgc gaccggctgc
     6241 tcgcattgct ccgcgagggc gccggcgaaa aactgactgt tcacagcgag tcggccccgc
     6301 ggctgccaaa cacgctctcg atcagcttcc cgcgggcaac cggccacgag ctgctcgccc
     6361 gcattccgga gctgtgcgcc tcgaccggct ccgcctgcca cagccagacg ccggcgatgt
     6421 cgccgacctt agccgcgatg ggcgtttcca gcgaagtc
//
LOCUS       DASRJB010000863        10912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_814783, whole genome shotgun sequence.
ACCESSION   DASRJB010000863 DASRJB010000000
VERSION     DASRJB010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814783"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2393
                     /locus_tag="VFB80_22640"
     CDS             <1..2393
                     /locus_tag="VFB80_22640"
                     /inference="COORDINATES: protein motif:HMM:NF039750.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36652.1"
                     /translation="HPPEKSAALARHFRDGLTDTERDWKPESALDDLLHALGCLRWGL
                     ARVDFDKLDLKLQTGLDEAPTPAPLEPPPPSSARDLIATLQDDPELGAVARLAHLLLA
                     AVQLPRALSDPDELPIGGVSDIANRGSLDRLLLSELASDDLTLAVRVAMNEALYLRRE
                     SPPKTPPRQRRLLLDAGLRTWGVPRIFVAAVGLAVAAKTDAAIRVEAFRAADGAAAPV
                     DLNSADGLKQHLAALDHRLHPAASVASLSRAESDDPEMESDLIVVTTDDTLADADFHR
                     ELRAAEVSGLFLASVSRDGRFELAQKGLRGSKVICRAKFDLDEVLKPRPKSQKLLDPA
                     RGDLPAFLQEPEPPLRFSCQVDVARSWLVHPQTVISYTRDGRLLLWDDVERGARQVAS
                     GLAAAGTLLWCDSLWDHEQRVRLVIGKRGRRGLWAVTLDRSGKANAVQLQLTGEHPQE
                     VIGLPGAALVIHNNLMEAMSWRDGELLACRSLHLPQRRGRFFSVGFGPSLGPNAVGRE
                     WRALSYHADRGDLSIHPELVYREPVGEPEPLLVVSDCVGVEGPVGVTAHGRLIDLATG
                     MERHSFRRQLPAGTRTPLTLTAISRDGQRFLVRGFCDAPGQATRVNQLLLDSAPTRRR
                     VTVVRDDPSVLEDPMLAITRPRVTHSKFRAIGLYGGGLALLSRRGQAWPILHHVGQGQ
                     LRFAAQPLPGGRDLTLDNKAAFQPVESWSGGWSLERAQWQGGSVAWLDGRGLLHLRSS
                     DQSLPEITLVLCDGPMAGWLSDGQSFGATYWIDRGVVATVNEVYASVIQRFIRQLA"
     gene            2390..5248
                     /locus_tag="VFB80_22645"
     CDS             2390..5248
                     /locus_tag="VFB80_22645"
                     /inference="COORDINATES: protein motif:HMM:NF039752.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36653.1"
                     /translation="MNVTLQLRYCPAPQRTAAAWLIPGGDVAAWLAELTAWNVPLAEA
                     RLLPLADGLLVWFPKPVPPIAGAAQAYGRAAEQVLLPVEAAIEPPVTERELARLLSPD
                     GAHYVWHPRHGLARYEPAAVQRIANLLAPPKPTGISWDAAQSGVAFNQRLLSIEPTQR
                     PTLEDLLEAARDGIATDPMAGGKLPAQPGEPIGGAIGQAVAQAGLTAAAAAAGAMGAA
                     ASAMAAGMQSLAGALTGAGQPTGSTAAQSRQSQASGAGGSNWLSRLAEWAKAQQQAAA
                     SNLDRLRNRQIERLLNMLKDTPDLGLRFAIPFGGEAYRGLSRPGASLTERNVDFSLAR
                     LGGGSSADFWTLSDEYRRKLLEQYRALANREISLGRHRRAAYIFAELLGDLTSAAATL
                     ASGGHFREAAILYEQRLNQPLSAAKCLQQGGLFAEAIAIYEKLAQWEIVAELYDKLDQ
                     REAARQALRRAVTARLTASDYLGAANLLEHKLQHTDEAQATLLTGWPRSAQAAACLDA
                     SFDLLARHGRHDEARRQVTALKPLTGTEQLQSLAAVLARAARKYPQPEVGHAAAEAAR
                     GLVAERLPRAATAETKNLLDALQSLVPADKLLGRDCGRYLAQRSADDAERRRQSLGSR
                     LKRPELRVASWFHLPPAEWKTAVSIGDQFYAAGWHESRLMIVRGQWDGTIQHLSVPVA
                     AGNAAMRSRPILLAADPWGAEPLYVYVAPGVPGSLASLTETDKFHRPVAVTPHSGCDV
                     STRVLTYGPGNTVLVGRLIGEGGAELALLLTPYENQRPLPGVQGIRFELEDLPDLDYI
                     QREPLPFFCRAADHYLGIGPRLIHKRIRQTMCELPSPIVSITGSAPHTRPRIVVGCEA
                     GGAILWGDTADAPHTTFATDLVEPAVGLNRGGWLAAATEDSVGVFTTEGGKLRFVSDS
                     DGPQARPLAVLSTNDHQRFAIVTATGKVMIFEVPARRT"
     gene            5400..6515
                     /locus_tag="VFB80_22650"
     CDS             5400..6515
                     /locus_tag="VFB80_22650"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36654.1"
                     /translation="MFRMESRKLRLRGFTLVELLVVIAIIGVLVALLLPAVQAARESA
                     RRTSCTNNIKQLGLAAHNFADVFGRFPEGVQIANPALTGAAREQNISAYRTPGFGPNW
                     AVIMLPFYEQKNLYELNSTGINNFKLTNGTDQSWRAIRSTRLTVFRCPSDPNHRAMFT
                     LNLTPPNGNNFGNWARGNYAGNAGAGWLHFTMEGLSFDGGAQSGSAPNSLVGGPFGVN
                     YGCRLTELTQQDGSSSTILFNEVRVGLNDVDRRGTWAMGVGGASLTCANAIGDATQPN
                     DANEFSDDIENCNDTRMKAGIPARSGMGPTRMGCSNDNLPNNWPNWQAQARSLHPGGV
                     QVCLGDGSVRTIPNTVAQSTWRLLNSRNDGQVIPNFD"
     gene            6532..6933
                     /locus_tag="VFB80_22655"
     CDS             6532..6933
                     /locus_tag="VFB80_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36655.1"
                     /translation="MQFSIYRKAAVCAMLSLAWLAGCGPPPNVTAKGTVLRNNQPVKM
                     MSPNGFIQVTLKPDVPPNQEFTTKVGRCNPDGTFEVLDVPPGRYVVGIEHLDPNPVSD
                     MLEGKLTYNFSKIKREVDGKTPILIDLAKPE"
     gene            7223..8077
                     /locus_tag="VFB80_22660"
     CDS             7223..8077
                     /locus_tag="VFB80_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HZN36656.1"
                     /translation="MNEASSAAPSKSAGFLARHEFLLRRLHSLSGLIPVGAYMVIHLL
                     TNASVLQSPAAFQRNVYSIHALGPLLLLIEWGFIFIPILFHAVFGVLIINGGLPNSST
                     YKYTNNVRYTLQRATGIIAFLFIVWHVFHMHGWIHAKWWEDGVAHYLYGGMFKPFSAA
                     STGAEAMRISWIVPILYAIGVLSCVFHLANGIWTFGITWGLWVTPASQRWANAVCLVF
                     GLGLSVVALGALGGFMAVDVNKALEAEDTMYDHKVAAKEIEPNEHKRRKASPKPQQAA
                     APSPSPQP"
     gene            8114..10123
                     /gene="sdhA"
                     /locus_tag="VFB80_22665"
     CDS             8114..10123
                     /gene="sdhA"
                     /locus_tag="VFB80_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZN36657.1"
                     /translation="MPKQRVLMVGGGLAGLAAAMKLAELGIDVDLMSLVPVKRSHSVC
                     AQGGINSVNDLTRQQGDDEWLHFDDTVYGGDFLQHQPPVKEMAYWGPRIIDLMDRLGV
                     TFNRTPEGYLDRRRFGGTLFKRTAFAGATTGQQLLYALDEQVRRWETEGKIRKFEFWD
                     FLGPIIDDGGVCRGAVAQDLVSMEIRSFPADAVIVGSGGPGLIYGRSTMSMACTGSAA
                     SRCFQVGAKYANGEFIQVHPTAIPGADKLRLMSESARGEGGRVWVPRKPQDPRDPLTI
                     PEKERFYFLEERYPKYKNLVPRDIATREIFDICVNEGLSVEKDRQCVYLDLTHIPRAE
                     LNRKLGGILEIYEKFQGVDPRSTPMKIFPAVHYSMGGLWVDYKKGTEGGLTIGSPQNQ
                     VTNIPNLYAIGECDYQFHGANRLGANSLLSCIFSGLIVAPGIEALLKGQKQAASELPS
                     SLFEQARKQHQSRHDALLKRSGGGENPYLIHQELGDVMTKVATVVRRNDQLDSGIAKV
                     HELYDRARKCSLSDSGNWTNQNVVFTKSLLDMFPLALTILKGARVRDECRGAHYKPAF
                     AFQPLKSTNPVERRREAEEWCDRFEANNTKWLKSTIAQWSGDDVEISFEDVDTSLIPP
                     RPRLYGLVGADEIERVWKERQTAQQPADGDGNGSAKPTKAVAAVH"
     gene            10161..>10912
                     /gene="sdhB"
                     /locus_tag="VFB80_22670"
     CDS             10161..>10912
                     /gene="sdhB"
                     /locus_tag="VFB80_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZN36658.1"
                     /translation="MASHSHNGHDHADGGQDSRPASFEVRILRQDGPGQPSYWERHTV
                     AYEPEMNVISVLQRIAAKATTSDGKKVAPVAWDCNCLEEVCGACTMLINGQVRQSCSA
                     LVDRLLADRPGEIELAPMTKFPVIRDLWVDRSRLFRALAEVKAWIPADGYYDQGPGPR
                     ISQAAQEAMYPLSECMSCGCCLEACPQYLKIEIERLNGESDEALEKRKIAEYDRGFIG
                     AHAISQAVLFNLNPTGKNNAAERLDALMDEGG"
BASE COUNT         1875 a         3576 c         3613 g         1848 t
ORIGIN      
        1 gtcatccgcc cgagaaatcg gcagccctcg ccaggcattt tcgcgacggc ctgaccgaca
       61 ccgagcgcga ctggaagccc gagtcggccc tcgacgacct gctgcacgcc ttgggctgcc
      121 tccgctgggg cctggcccgc gtcgatttcg acaagctcga tctgaagctg caaaccgggc
      181 tggacgaagc gccgacgccg gccccgctcg agccgcctcc cccttcttcg gcccgcgact
      241 tgatcgccac gctccaggac gatccggagt tgggcgcagt cgcgcggctc gcgcacctgc
      301 tgctggccgc ggtgcagctg ccgcgggcgc tctcggatcc cgacgagctg cccatcggcg
      361 gcgtgagcga catcgccaac cgcggctcgc tcgaccggct gctgcttagc gagctggcca
      421 gcgacgactt gaccttggct gtccgcgtgg cgatgaacga ggccctgtac ttgcgccgcg
      481 agtcgccgcc gaagactccg ccccggcagc ggcggctgct gctcgacgcg ggactgcgaa
      541 cgtggggcgt gccgcggatt ttcgtcgctg ccgtcggact ggccgtggcg gccaagaccg
      601 acgctgcgat ccgcgtcgaa gcgttccgcg cggccgacgg ggccgcggcg cctgtcgatc
      661 tgaactcggc cgacggattg aaacaacacc tggcggcgct cgaccatcgg ctgcatccgg
      721 ctgcttccgt ggcgtcgctc agccgggccg agtcggacga ccccgaaatg gagtcggacc
      781 tgatcgtcgt tacgaccgac gatacgctcg ccgacgccga cttccatcgc gagctgcggg
      841 cggcggaagt ctccgggctg tttctagcgt cggtcagccg cgacgggcgt ttcgagctgg
      901 cccagaaggg cctccgaggc agcaaggtca tctgccgggc gaaattcgac ttggatgagg
      961 tcctgaagcc gcggcccaag tcgcaaaagc tgctggaccc agcgcgcgga gatctgccgg
     1021 catttctcca ggagcccgag ccgccgctgc ggttttcgtg ccaggtcgat gtcgcccgct
     1081 cctggctggt gcatccgcag accgtcatca gctacacgcg cgacggccgg ctgctgctct
     1141 gggacgacgt ggagcgtgga gcgcggcagg tggccagcgg gctcgcggcc gcggggacct
     1201 tgctctggtg cgattcgctt tgggatcacg agcagcgcgt gcggctggtc atcggcaagc
     1261 gcggccgccg cggcctgtgg gccgtgaccc tggaccgcag cggcaaggct aacgctgtcc
     1321 agttgcaact gaccggcgag cacccgcagg aggtcattgg cctgccaggc gcggcactgg
     1381 ttattcacaa caacctgatg gaagcgatgt cgtggcgcga cggagaactg ctggcctgcc
     1441 gctctctgca tttgccgcag cggcggggcc ggttcttcag cgtgggcttc ggcccgtccc
     1501 tgggccccaa cgcagtggga cgtgagtggc gggcgctgtc ctatcatgcg gaccgcggcg
     1561 acttgagcat ccatccggag ctggtctacc gcgagccggt cggcgagccg gaaccgctgc
     1621 tcgttgtctc ggactgcgtg ggcgtggaag ggcccgtggg agtgaccgcg cacggccggc
     1681 tcatcgacct ggccacgggc atggagcggc actcgttccg gcgacaatta cccgcgggca
     1741 cgcgcacgcc gctgacgctg acggccatct cgcgcgacgg tcagcggttc ctcgtccgtg
     1801 gattctgcga tgcaccaggc caggccacgc gagtgaatca attgctgctg gattccgcgc
     1861 caacacggcg ccgcgtgacc gtcgtgcgcg acgatcccag cgtcctggaa gacccgatgc
     1921 tggcgatcac gcggccgcgc gtaacgcact ccaaattccg ggcgattggg ttgtatggcg
     1981 gcgggctggc gctgctgtcg cggcgcgggc aggcctggcc gattctgcac cacgtcggcc
     2041 aggggcagtt gcgattcgcg gcgcaaccgc ttccgggcgg ccgcgacctg acgctggaca
     2101 acaaggcggc attccagccg gtcgagtcct ggtcgggcgg ctggtccttg gaacgagccc
     2161 agtggcaggg cggcagcgtc gcctggctcg acggccgcgg cctgttgcac ctgcgcagct
     2221 ccgaccagag cctgcccgaa atcacactcg tgctgtgcga cgggccgatg gccggctggc
     2281 tctcggatgg gcagtcgttc ggcgcgacgt attggatcga tcgcggcgtg gtcgccacgg
     2341 tcaacgaggt gtacgcatcc gtcatccagc gattcatccg ccaactggca tgaacgtcac
     2401 gctccagctt cgctattgcc cagcgccgca gcgcacggcc gccgcctggc tcattcccgg
     2461 cggcgacgtt gcggcctggc tggccgagct gaccgcctgg aacgtgccgc tggccgaggc
     2521 cagactgctg ccgctggcag acggtctctt ggtctggttt cccaagccgg tgccgccgat
     2581 tgccggcgcg gcgcaagcct acggccgggc ggcggagcag gtcctcttgc ccgtcgaagc
     2641 cgccatcgag ccgccggtga cggagcgcga gctggcgcgt ttgctatcgc ccgatggcgc
     2701 gcactacgtc tggcatccgc gccacggtct ggcccgttat gagcccgccg ccgtgcagcg
     2761 aatcgccaac ctgcttgcgc cgcccaagcc gaccggaatc tcgtgggacg ccgcacagtc
     2821 cggtgtggca ttcaaccagc gattgctgtc gatcgagccg acgcagcggc cgacgctgga
     2881 agacctgctg gaggccgctc gcgacggcat cgccaccgac ccgatggccg gcggcaaact
     2941 gccggcgcag cccggcgagc cgatcggcgg cgcgattgga caggccgtgg cccaggccgg
     3001 cttgacggcg gccgctgcag cagccggggc gatgggcgcg gccgcctcgg cgatggccgc
     3061 gggtatgcag agcctggcgg gcgcactcac cggcgccggg cagccgaccg gttccaccgc
     3121 ggcccagtcg cggcagtctc aggcgtctgg cgcgggcggc tcgaactggc tgtcgcgctt
     3181 ggcggaatgg gccaaggccc agcagcaggc tgcggccagc aacctcgacc ggctccgcaa
     3241 tcggcagatc gagcggctgc tgaatatgct caaggacacc cccgacctgg gcctgcgatt
     3301 cgccattcca ttcggcggcg aggcttatcg cggcctgtcg cggccggggg cgagcctgac
     3361 cgagcgaaac gtcgatttca gcctggcccg cctgggcggc ggctcttcgg cagatttctg
     3421 gaccctctcc gacgagtatc gccgcaagct gctggagcag tatcgggcgc tggctaatcg
     3481 cgagatttca ctgggccggc accgccgcgc ggcgtacatc tttgccgagc tgctgggcga
     3541 tttgacttcc gcagcggcga cattggccag cggcgggcac tttcgcgagg cggcgatctt
     3601 gtacgagcag cggctgaatc agccgctctc ggccgccaag tgcctgcaac agggcggcct
     3661 gtttgccgag gcgatcgcga tctacgagaa gctcgcgcag tgggagattg tggcggagct
     3721 gtacgacaag ctcgaccagc gcgaggccgc ccggcaggcg ctgcgccgag cggtcacggc
     3781 gcgtctgacc gcctccgatt acctgggcgc ggcgaacctg ctggagcaca agctgcagca
     3841 cacggacgag gcgcaggcca cgctcctgac cggctggccg cgatcggccc aggcggccgc
     3901 ctgcctggac gcttcgttcg accttctcgc ccgccacggc cggcacgacg aggctcggcg
     3961 gcaagtcact gcgctaaagc cgctgaccgg cacggagcag ctgcaatcgc tggcggcggt
     4021 gctggctcgg gccgcgcgaa aatatccgca gccggaagtc ggccacgcgg cggccgaggc
     4081 ggcgcgcggg ctggtggcgg agcggctgcc gcgagcggcg accgcggaga cgaaaaacct
     4141 gctcgacgcg ctgcaaagcc tcgtgccggc cgacaagctg ttgggacgcg actgcggccg
     4201 ctacctggcg cagcgttcgg cggacgatgc cgagcggcgg cggcagagcc tcggctcacg
     4261 cctcaagcgc ccggagctgc gcgtcgccag ctggtttcac ctgcccccgg cggagtggaa
     4321 gacggccgtc agcatcggcg atcagttcta cgcggcgggc tggcacgaat cgcggctgat
     4381 gatcgtccgc ggccaatggg atggcacgat tcaacatctg tcggtcccgg ttgcggcggg
     4441 gaacgccgcg atgcgctcgc gcccgatcct gcttgccgcc gatccgtggg gcgcggagcc
     4501 gctgtacgtc tatgtcgcgc cgggcgtgcc cggctcgctg gcctctctga ctgagaccga
     4561 caagttccat cgccccgtgg ccgtcacgcc gcattccggc tgcgacgtgt ccacccgcgt
     4621 gctgacctat ggcccgggta acacggtgct cgtgggccgc ctgataggcg aaggcggcgc
     4681 cgagctggcc ctgctgctga cgccctacga aaaccagcgg ccgctgccgg gcgtgcaggg
     4741 aatccgcttc gagttggagg atttgcccga tctggactac atccagcgag agcccctgcc
     4801 gtttttctgc cgggcggcgg accattacct ggggatcggg ccgcggctga tccacaagcg
     4861 aatccggcag acgatgtgcg agctgccttc gccgatcgtc agcatcacgg gctccgcccc
     4921 gcacacgcgg ccgcggatcg tggtgggctg cgaggcgggt ggcgcgatcc tgtggggcga
     4981 cacggcggat gcgccgcata ccacgtttgc gacggatctc gtggagccgg ccgttggcct
     5041 caaccgcggc ggatggcttg ccgcggcgac cgaagattcg gtcggcgttt tcacgaccga
     5101 ggggggcaag ctgcgcttcg tatccgatag cgacgggcct caggcccggc cgctggcggt
     5161 cctttccacg aacgatcacc agcgattcgc catcgtgacg gccaccggga aggtgatgat
     5221 cttcgaagtt ccggcccggc ggacttagcg cgagtctttc ccgcccgcga gcattaaaac
     5281 tggccggttc gatttgacat cgcccgcaac ggttgccaga atcacgcttt agaggcgaca
     5341 agcgctagtt tgtcgttttc acgcgattct catcttgcac tcgccttggg ggacgggcca
     5401 tgttccgcat ggaatctcgg aagctgcgtc tgcgcggctt cacgctggtg gaattgctgg
     5461 ttgtcattgc gattattggc gtacttgtgg cgttgctgct gcctgccgtg caagccgccc
     5521 gggaatccgc gcggcgaacg agctgcacaa acaacatcaa gcagctcggg ttggccgcgc
     5581 acaatttcgc cgatgtgttt ggccggtttc cggaaggcgt gcaaattgcc aatcctgctt
     5641 tgaccggggc tgcgcgggaa cagaacatta gcgcctatcg gacgcccggc ttcggcccga
     5701 attgggccgt gatcatgctg cctttctatg aacagaaaaa tctgtacgaa ctcaactcga
     5761 caggcatcaa caacttcaag ttgaccaacg gaactgatca gagttggcga gccatccgct
     5821 cgacgcggtt gaccgtattt cgctgcccat cagatccgaa ccaccgggcg atgttcacgc
     5881 tgaatctcac cccacccaat ggcaataact ttggcaactg ggctcgcggc aattacgcgg
     5941 gaaatgccgg tgccggctgg ctgcatttta cgatggaggg tctgtccttc gacggcgggg
     6001 ctcaatctgg aagtgcgcca aacagcctgg taggagggcc attcggcgtc aactacggtt
     6061 gtcgattgac cgagttgaca cagcaggacg gctcctcaag taccattctg ttcaatgagg
     6121 ttcgcgtggg gttgaacgat gtcgatcgcc gcggaacttg ggcgatgggt gtgggcggcg
     6181 ccagtctgac ctgcgccaac gcgatcggcg atgccacgca acccaacgac gccaacgagt
     6241 tctctgatga catcgaaaac tgcaacgaca cccgcatgaa ggctgggata ccagcccgga
     6301 gcggaatggg cccgaccaga atgggctgtt ccaacgacaa cctgcccaac aactggccca
     6361 actggcaggc gcaagcccgc agcctgcatc ccggcggcgt tcaagtctgc ctgggcgacg
     6421 gcagcgtccg gacgatcccg aatactgttg cgcaaagcac ctggcggctg cttaacagcc
     6481 gtaacgacgg ccaggtaatt cccaatttcg actaacagtg agtgagctgc gatgcaattc
     6541 tcgatttatc ggaaggcggc ggtctgcgcg atgttgtcgc ttgcctggct ggcgggctgc
     6601 gggccgccgc ccaacgtcac ggccaagggc acggtgttgc gaaataatca gcccgtcaag
     6661 atgatgagcc ccaacggctt catccaggta accctgaagc ccgatgtgcc gcccaaccag
     6721 gaattcacga ccaaggtcgg ccgctgcaat ccggacggga cctttgaagt cttggacgtg
     6781 cccccgggtc gctatgtcgt cggcattgag cacctcgatc ccaacccggt ctcggatatg
     6841 ctcgaaggca agctgacgta taacttcagc aagatcaaac gcgaagtcga tggcaagacg
     6901 ccgatcctga tcgacctggc caagccggaa taggcagcgc ccgcggcctg tgaggtacat
     6961 cacggaagcg ggcggcgtgt gtttctacat gccgccggac gcccgggaaa aaatcgggcg
     7021 gcagctgttt gctgccgagc gggcgcggct ggcagtcggc ggcaaatagc aacgtcggca
     7081 gcgtccgatg tcccgttgtc agtcgaatcg ggacttctat actggtgcgg gctgtcgctt
     7141 cccaggccca tgccgtctgc cagaatccct gaatcggctg taaattgaag gcagacttgc
     7201 ccttgcgtct gaaaggattg ccgtgaacga ggcctcctcg gctgcacctt ccaaatctgc
     7261 cggtttcctg gcccgccacg agtttctgct tcgccggctg cattccctct cggggctgat
     7321 tcccgtgggg gcgtacatgg tcatccacct gctgacgaat gccagtgtgc tgcaaagccc
     7381 ggccgcgttt cagcggaacg tctattcaat tcatgccctg ggcccgctcc tgttgctgat
     7441 cgaatggggc ttcatcttca tcccgatcct gttccacgcc gttttcggcg tgctgatcat
     7501 caacggcggc ctgcccaaca gcagcacgta caaatacacg aacaacgtcc gctacacact
     7561 gcagcgggcg accgggatca tcgccttttt gttcatcgtc tggcacgtgt tccacatgca
     7621 tggctggatt cacgccaagt ggtgggaaga cggcgtggcc cactacctgt atggcgggat
     7681 gttcaagccc tttagcgcgg cctcgaccgg cgcggaagcc atgcgaatca gctggatcgt
     7741 cccgattctt tacgcaatcg gcgtgctgtc gtgcgtgttt cacctggcca acggcatctg
     7801 gacgttcggc atcacctggg gcctgtgggt cacgcccgcc tcgcagcggt gggccaacgc
     7861 ggtctgcctt gtgttcggtc tcggtttgag cgtcgtggct ttgggagctc tgggcggctt
     7921 tatggcggtc gatgtgaaca aggccctgga agccgaagac acgatgtacg atcacaaggt
     7981 cgccgccaaa gagatcgagc ccaacgagca caaacgcaga aaagccagcc ccaagccgca
     8041 acaagccgcg gcccctagcc ctagccccca gccctagccc gggaacgccg cagagggcgt
     8101 tccctacagc aatatgccta agcaacgcgt tctgatggtc ggcggcggtc tggcggggct
     8161 ggcggccgcg atgaagctgg ccgaactggg gatcgatgtc gatctgatga gcctggtgcc
     8221 cgtcaagcgc tcgcacagcg tctgcgctca aggcggcatc aacagcgtca acgacctgac
     8281 gcggcagcaa ggcgacgacg agtggctgca cttcgacgac acggtttacg gcggcgactt
     8341 cctgcagcat cagccgccgg tcaaggaaat ggcctattgg gggccgcgga tcatcgacct
     8401 gatggaccgg ctgggagtga cgttcaaccg cacgcccgag ggctatctcg atcggcgacg
     8461 gttcggcggc acgctcttca agcggacggc ctttgccggc gcgaccaccg gccagcagct
     8521 cctgtacgcc ctggacgagc aggtccgccg ctgggagacc gagggcaaga tccgcaagtt
     8581 cgagttctgg gattttctgg gcccgatcat cgacgacggc ggcgtctgcc gcggggcggt
     8641 cgcgcaggac ctggtttcga tggagatccg gtcctttccg gccgacgcgg tgatcgtcgg
     8701 cagcggcggc ccgggtctga tctatggccg ttcgacgatg tcgatggcct gcaccggcag
     8761 cgccgccagc cgctgcttcc aggtcggggc caagtacgcc aacggcgagt tcatccaggt
     8821 ccatccgacg gcgattcccg gcgccgacaa gctccgcttg atgagcgaat cggcccgcgg
     8881 cgaagggggc cgcgtgtggg tgccgcgcaa gccgcaagac ccgcgcgatc cgctgacgat
     8941 tcctgagaag gagcggtttt atttcctgga agagcgctat ccgaaataca agaacctcgt
     9001 gccgcgcgac atcgcgaccc gcgagatctt cgacatctgc gtgaacgaag ggctgtcggt
     9061 cgagaaggac cgccaatgcg tctatctcga cctgacgcac attccccggg cggagctcaa
     9121 ccgcaagctg ggcggcattc tggagatcta cgaaaagttc cagggggtcg atccgcggtc
     9181 cacgccgatg aagatcttcc ccgccgtgca ctattcgatg ggcgggttgt gggtcgatta
     9241 caaaaagggc accgagggcg gactgaccat tggctcgccg cagaaccagg tcaccaacat
     9301 tccgaacctg tacgcgatcg gcgagtgcga ctatcaattc cacggcgcca atcgcctagg
     9361 cgcgaactcg ctgctctcgt gcatcttcag cgggctgatc gtcgccccgg ggatcgaggc
     9421 gctcctcaag gggcagaagc aggcggccag cgagctgccg agcagcttgt tcgagcaggc
     9481 ccgcaagcag caccagtcgc ggcacgacgc gctgctgaag cggtcgggag gcggtgagaa
     9541 cccgtacctg attcaccagg aattgggcga cgtgatgacc aaggttgcca ccgtcgtccg
     9601 ccgcaacgac cagctggata gcggcatcgc gaaggtccac gagttatacg accgggcccg
     9661 caagtgctcg ctctctgaca gcggcaactg gacgaaccag aacgtcgtct tcaccaagtc
     9721 cctgctcgac atgttcccgc tggcgctcac gattctcaag ggcgctcggg tccgcgacga
     9781 atgccgcggc gcgcactata agccggcgtt cgcctttcag ccgctgaaat cgaccaatcc
     9841 cgtcgagcgc cgccgcgagg ccgaggagtg gtgcgaccgc tttgaggcca acaacaccaa
     9901 gtggctcaaa tcgacgatcg cccagtggtc cggcgacgac gtggagattt ccttcgaaga
     9961 cgtcgatacc tcgctgattc cgccccgccc gcggctgtac ggcctcgtcg gcgcggacga
    10021 gatcgagcgg gtctggaagg aacgccagac cgcccagcaa ccggccgatg gcgatgggaa
    10081 cggaagcgcg aagccaacca aggccgttgc cgcggtccac tagggaactc aatcccaaat
    10141 cttgaaatct gaaatctgaa atggcctccc attcccacaa cggtcacgac cacgcggacg
    10201 gcggccaaga ttcccgcccg gcgtcgttcg aggtccgcat cctgcggcaa gacggccccg
    10261 gtcagccaag ctattgggag cggcataccg tcgcctatga gccggagatg aacgtcatca
    10321 gcgtgctcca gcgaatcgcc gccaaggcga cgactagcga cggcaaaaaa gtcgcccccg
    10381 tcgcctggga ctgcaactgc ctggaagagg tctgcggcgc ttgcacgatg ttgatcaacg
    10441 gccaggtccg ccagtcctgt tcggccctcg tcgataggct gctcgccgac cggcccggtg
    10501 agatcgagct cgcccccatg accaagttcc ccgtcatccg cgacttgtgg gtcgatcgct
    10561 cgcggctgtt ccgtgccctc gccgaggtca aggcctggat tcccgccgac ggttattacg
    10621 accaggggcc cggaccgcgg atttcgcaag ccgctcagga ggcgatgtac ccgctcagcg
    10681 agtgcatgag ctgcggctgc tgcctggaag cctgcccgca atacctgaag atcgaaatcg
    10741 agcgcctgaa cggcgaatcg gatgaagcgc tcgagaagcg aaagatcgcg gaatacgacc
    10801 gcggctttat cggcgcgcat gccatcagcc aggccgtgct cttcaacttg aatcccacgg
    10861 gcaagaacaa cgccgccgag cggttggacg ccctgatgga cgaaggcggc at
//
LOCUS       DASRJB010000864         5333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2779558, whole genome shotgun sequence.
ACCESSION   DASRJB010000864 DASRJB010000000
VERSION     DASRJB010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2779558"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1133
                     /locus_tag="VFB80_22675"
     CDS             39..1133
                     /locus_tag="VFB80_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36659.1"
                     /translation="MIGLGFGAEFIPIYQAHPSANMYAICQRNEAKLNKVGDTFGIGT
                     RYTEYEDVLADSQVDFVHINSPIPDHAWMSIAALKAGKHVMCTVPMATTIKECDKICQ
                     LVADTGLTYMMAETVVYSREFLFIKQMYQKGELGKIQHLAASHPQDMDGWPDYWQKMI
                     PMHYATHVVSPCLGLMEKPAEYVSCFGSGTVRKDIAKKSGNAYAVESCHIKIKGSDVV
                     AHIWRFLYDVARQYRESFDVYGTKRSFEWTLVEHQPHTLHTAKQPEPEIPSAIEVTDF
                     AHLLPEPIRRFTQPAEIHDAEHLSFIQGGGHGGSHPHMVNEMVTALVKKRDPWPNATT
                     AANWTCVGLCAHQSAEKGGKIVKLPAFTLK"
     gene            1210..1812
                     /locus_tag="VFB80_22680"
     CDS             1210..1812
                     /locus_tag="VFB80_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36660.1"
                     /translation="MWIRILIAGIAGGVLVFVTGAFSHAALGLQGRTIENLPDEATFV
                     EQVKGQKLRHGLYGFPGQPTSKDAKEREREFAEVQKRFKAGPAGFLLIAPTGEEMMGG
                     ATLGMEFATNAIAALLAAWILSLAAADVGFGKRWLAVLAMGIFAWFSLSASYGIWYRF
                     PHDFIHDEFYCAVLEWGVAGLAIAAIVRKPIQPAQGTKHE"
     gene            1805..4033
                     /locus_tag="VFB80_22685"
     CDS             1805..4033
                     /locus_tag="VFB80_22685"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN36661.1"
                     /translation="MSKPIASFVTSAWLMCLAATALAQPPAEKQEDQSPKFSAPPEGF
                     DVRRDGAQAGKLERVDYDSRTVGVKRWMQVYTPPGYSKDQKYPVLYLLHGIGGNEREE
                     WTRGGVAHVVLDNLIADKKIEPLIVIFPNGNATASAAAPAPGKAAPGGAAPGGAAPGR
                     GGPGGRRGGFGGWGTPFENDLIKDIIPFIEANYSVKADREGRAIAGLSMGGGQALNIG
                     LANLDTFAWVGGFSSAPNTRQPTELIKDHAEAAKKLKLLYVACGDADGLLRISQGVHG
                     MLDEKKVPHLYNVIPGGRHDFKVWKSDLYHFTQLIFREPGQAAAAAKPAEEAKPAPAQ
                     PAPAQPAAQPAPAEAEPVAEDFKPAHSNQNGRQYPQVNSQRRARFRIVAPQAQSVRVP
                     EWGGIALTKGEDGAWVGTTRPLDEGFHYYRINIDGADVPDPGSKFFYGANRWGSAIEI
                     PAHDEDFYAVENVPHGQLRETLYFSKSTNATRRCFVYTPPDYDKNLETRYPVLYLQHG
                     AGEDETGWGSQGRTNLIMDNLIAAGQARPFIIVMDNGGNIGGGARRGGPPGPGGPGAV
                     PGRGGAGGAAGGPGGRGGFGGRGGPAFNFSAFATILTEELIPFIDAHYRTLSDQPHRA
                     MAGLSMGGFQTRQIALANLDKFSHIGIFSGGSIAADDPALADPAAFKQKVKVLFVSYG
                     SKENTATAKANHEALAQKGVPNTFYVSEGTAHEWQTWRRSLHQFAPLLFRDSDPTVAK
                     EQ"
     gene            4111..4950
                     /locus_tag="VFB80_22690"
     CDS             4111..4950
                     /locus_tag="VFB80_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36662.1"
                     /translation="MIFLLAGSTALAQAPASRPPAGLKIFYTGHSFHMFVPQHVNVLV
                     KAAGIEGHLEVGRQGIGGSRVIQHWDKADDQNKAKQALMTGEVDVFTMAAHVQIPDEG
                     ITKFTELGLAHNPKLRLLVQASWFPFDVPEPEKRIRENAQRDNMAIADLQKAVDDWRK
                     KLEAQADDLNRQNGKTCVFIVPVGDAMVKLRSLIVDGKYPGVAKQSELFSDPIGHGGG
                     HVQALASYCNFVAIYRHNPLGLKMDVAGVSDEQHAILQKIAWETVSEYPYAGIAAKAA
                     AAK"
     gene            4987..>5333
                     /locus_tag="VFB80_22695"
     CDS             4987..>5333
                     /locus_tag="VFB80_22695"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN36663.1"
                     /translation="MKRLSLVAALGLSLALACSAHSAEPLKLLFLGDSGHHQPAARFR
                     QLAPAMKERGIELTYTDKMDDIDAENLGKYAGLVIYANTTRISPEQEQALLDYVAGGK
                     GLIPLHCASYCFL"
BASE COUNT         1039 a         1695 c         1716 g          883 t
ORIGIN      
        1 tggccaaacg agctgcgggc aagcaagtca acgtcgcgat catcgggctg gggttcgggg
       61 cggagttcat tcccatctat caggcgcatc ccagcgccaa catgtacgcg atctgccagc
      121 ggaacgaggc caagctcaac aaggtaggcg atacgtttgg catcgggacc cggtacaccg
      181 aatacgagga cgtgctggcc gattcccagg tcgatttcgt ccatatcaac tcgccgatcc
      241 ccgaccacgc ctggatgtcg attgcggccc tcaaggccgg caagcacgtg atgtgtaccg
      301 tgcccatggc cacgaccatc aaggagtgcg acaagatctg ccagctcgtg gccgacaccg
      361 gcctgaccta catgatggcc gagacggtgg tctacagccg cgagttcctg ttcatcaagc
      421 agatgtatca gaaaggcgag ctgggcaaga ttcagcatct ggccgcgtcg cacccgcagg
      481 acatggacgg ctggcccgac tactggcaga agatgatccc gatgcactac gcgacgcacg
      541 tggtcagccc gtgcctcggg ttgatggaga agccggccga atacgtgagc tgttttggca
      601 gcggcaccgt gcgaaaggac atcgcgaaga agtcgggcaa cgcctatgcc gtcgagagct
      661 gccacatcaa gatcaagggc tcggacgtgg tggcccacat ctggcggttc ctgtacgacg
      721 tggcccggca ataccgcgag agtttcgacg tctatggcac caagcggagt tttgagtgga
      781 cgctggtcga gcatcagccg cacacgctgc acacggcgaa gcagccggag ccggagattc
      841 cgtcggcgat cgaggtgacg gatttcgccc acttgctgcc cgagccgatc cgccggttca
      901 cgcagccggc ggagattcac gacgcggagc acctctcgtt cattcagggc ggcggccatg
      961 gcggctcgca cccgcacatg gtgaacgaga tggtcacggc cctggtgaaa aagcgcgacc
     1021 cctggcccaa cgcgaccacg gccgcgaatt ggacctgcgt cggcctgtgc gcccaccagt
     1081 cggccgagaa gggcgggaag atcgtcaagc tgccggcgtt tacgctcaag tgattgatcc
     1141 tcgttcccag gctccgcctg ggaacgcacg tccgcgaggc tcctgcctcg ctgggtgaaa
     1201 gacatagcca tgtggattcg catcctgatc gccggcatcg cgggcggcgt gctggtcttt
     1261 gtgacgggcg cgttcagcca cgccgcgctt ggcttgcaag gtcgcaccat cgagaacctc
     1321 cccgacgagg cgacgttcgt cgagcaggtc aaaggccaaa agctgcggca cggcctgtat
     1381 gggtttccgg gacagccgac cagcaaggac gcgaaggagc gggagcggga gtttgcagaa
     1441 gttcaaaagc gcttcaaagc cggcccggcg ggctttctgc tgatcgcgcc gaccggcgag
     1501 gaaatgatgg gcggcgccac gctggggatg gaattcgcca ccaacgcaat cgccgcgctg
     1561 ctcgccgcct ggatcttgtc ccttgcggct gccgacgtgg gttttggcaa gcgctggctg
     1621 gcagtgctgg cgatgggcat ctttgcctgg ttctcgctca gcgcctccta cggcatctgg
     1681 taccgcttcc cgcacgactt catccacgac gagttttact gcgccgtcct cgaatggggc
     1741 gtggccggcc tggcgatcgc ggcgatcgtg cggaaaccta tccagcctgc acaagggacg
     1801 aaacatgagt aaaccaattg catccttcgt tacgtcagcc tggctgatgt gcctggccgc
     1861 gacggcactc gcccagccgc cagcagagaa acaggaagat cagtcgccaa aattctccgc
     1921 gccgcccgag ggtttcgacg tccgccgcga cggcgcacag gccggcaagc tggaacgcgt
     1981 cgattacgat tccaggaccg tcggcgtgaa acgctggatg caggtctata cgccgcccgg
     2041 ctattccaaa gaccagaagt atcccgtgtt gtatctgctg cacggcatcg gcggcaacga
     2101 acgggaggaa tggacccggg ggggcgtggc gcacgtcgtc ctggacaacc tgatcgccga
     2161 caagaaaatc gagcccctga tcgtcatctt ccccaacggc aatgccacgg ccagcgctgc
     2221 ggcgccagct cccggcaaag cggccccagg cggcgccgct ccaggcggcg cggctccagg
     2281 ccgcggcggc ccaggcgggc ggcggggtgg attcggcggg tggggcacgc ccttcgagaa
     2341 cgacctcatc aaagacatca ttcccttcat cgaggccaac tactccgtga aggcggatcg
     2401 cgagggccga gccattgccg gactgtcgat gggcggcggg caagcgctca acatcggtct
     2461 ggccaacctg gacacgttcg cctgggtcgg cggattttca tcggccccga atacaaggca
     2521 gccgaccgag ctcatcaagg atcacgcgga ggcagccaag aagctgaagc tgctgtatgt
     2581 cgcctgcggc gacgccgacg gccttctcag aatcagccaa ggcgttcacg ggatgctgga
     2641 cgagaagaaa gtcccgcacc tgtataacgt cattccgggc ggccggcacg acttcaaagt
     2701 ctggaagagc gacctgtatc acttcacgca gttgatcttt cgcgagccag ggcaagcggc
     2761 agcggctgcg aagccggccg aagaagccaa gccagcgcca gcccagccag cacccgcgca
     2821 gcccgcagcc cagcccgccc cggcagaggc cgagcccgtt gcggaagact ttaagcctgc
     2881 acattccaac cagaacggcc ggcaataccc gcaggtgaat tcccagcgcc gggcccgctt
     2941 ccgcatcgtc gcgccgcagg cccaaagtgt gcgcgtgcct gaatggggcg gaatcgcgtt
     3001 gaccaaaggc gaggatggcg cctgggtcgg caccacgcgg cccttggacg aaggatttca
     3061 ctactaccgg atcaacatcg acggcgccga cgttcccgat cccggcagca agttcttcta
     3121 tggcgccaat cgctggggca gcgccattga aatcccggct cacgacgagg atttttacgc
     3181 cgtggagaac gttccgcacg gtcagctccg cgagaccctg tacttttcca agagcaccaa
     3241 cgccacccgc cgctgcttcg tctatacgcc gccagactac gacaagaatc tcgaaactcg
     3301 ctatccggtg ctctacctgc agcacggcgc cggcgaagac gagacgggct ggggcagcca
     3361 ggggcggacg aacctgatca tggacaacct gatcgccgcg ggccaagcgc ggccgtttat
     3421 catcgtgatg gataacggcg gcaacatcgg cggcggggcg cggcgcggtg gaccgcctgg
     3481 gcccggcggc ccgggcgcgg ttcctggccg cggcggtgcg ggcggagctg ccgggggccc
     3541 tggcggccgc ggtggttttg ggggccgcgg cggtccggcg ttcaacttct cggcctttgc
     3601 gacgattctt accgaggagc tgatcccctt catcgacgcc cactaccgca cgctctccga
     3661 tcagccgcac cgtgccatgg cggggctctc catgggcggc ttccagacac ggcagatcgc
     3721 gctggcgaac ctcgataagt tctcgcacat cggcatcttc agcggcggca gcatcgccgc
     3781 cgacgatcca gcactggccg atccggccgc tttcaagcag aaggtcaagg tgctgttcgt
     3841 gagctatggc agcaaggaaa atacggcaac ggccaaggcc aaccacgagg cactagcgca
     3901 gaagggtgtc ccgaacacgt tctacgtgtc ggagggcacg gcgcatgagt ggcagacctg
     3961 gcggcggagc ttgcatcagt tcgcgccgct cctgtttagg gacagtgatc caacggtggc
     4021 caaggagcaa tgatggtgac tgagcgaacg ctgccagcga cggcttcgta cccggcgcgg
     4081 agtgcatcgt gcctggtacg gcgctggatt ttgattttct tgttggccgg cagcacggcg
     4141 ttggcgcaag cgccggccag ccggccgcct gccggcctga agatcttcta caccggtcac
     4201 agctttcaca tgtttgtccc gcagcacgtc aacgtgctgg tgaaggcggc cggcattgaa
     4261 gggcacctgg aagtcggccg gcagggcatc ggcggctcgc gagtgatcca gcactgggat
     4321 aaggccgacg accagaacaa ggccaagcaa gccctgatga ccggcgaagt cgatgtcttc
     4381 acgatggccg cccacgtgca gattcccgac gaggggatca cgaaattcac ggagctgggc
     4441 ctggcgcaca atccgaaact ccggctgctt gtgcaggctt cgtggttccc gttcgacgtg
     4501 ccggagccgg agaagcggat tcgcgagaac gcccagcgcg acaacatggc tatcgccgac
     4561 ttgcaaaagg cggtcgatga ttggcggaag aagctggagg cgcaggccga cgacctcaac
     4621 cgacagaacg gcaagacgtg cgtgttcatc gtgccagtcg gcgacgcgat ggtgaagctg
     4681 cggagcctga tcgtcgacgg caagtatccg ggcgtggcga agcagtccga gctgttctcc
     4741 gacccgatcg gccacggcgg cggccacgtc caggcgctag cttcgtactg taacttcgtc
     4801 gcgatctacc gccacaatcc actgggcttg aaaatggacg tggctggcgt gagcgacgag
     4861 cagcacgcca tcctccagaa aatcgcttgg gagaccgttt ccgaatatcc gtatgcggga
     4921 attgcggcaa aggccgcagc cgccaagtag cccctcctca ccgattcagt acatcgcagg
     4981 tccgccatga aacgtctctc gctggtcgct gcgctcggtt tgtcgttggc tctcgcctgc
     5041 tcggcgcact ctgccgagcc cttgaaactg ctctttctcg gcgattccgg ccaccatcag
     5101 ccggcggcgc ggttccggca gctagcgccg gcgatgaagg agcgcggcat cgagctgacc
     5161 tacacggaca agatggacga catcgatgcc gagaatctcg gcaagtacgc cggcctcgtg
     5221 atttatgcca acaccacgcg gatctcgccc gagcaggagc aggcgctgct cgactatgtc
     5281 gccggcggca aggggctcat tccactgcac tgcgcgtcgt actgcttcct gaa
//
LOCUS       DASRJB010000865         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_255562, whole genome shotgun sequence.
ACCESSION   DASRJB010000865 DASRJB010000000
VERSION     DASRJB010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255562"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="VFB80_22700"
     CDS             <1..2063
                     /locus_tag="VFB80_22700"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZN36664.1"
                     /translation="QLFQARLAEKSSWLTRVAQDFDLRRYAFDTTLRPIKGFEELTLD
                     GQASAMQGSLTALAERFRGQPIAGILLFTDGNATDLAGNLDWSRLPPVYPVLPSASGE
                     LVDLSLARVSISQTNFEAAPVTITAEIDGQSVAGQKIVIRVLDEENKEVERRTLAAPA
                     GTEPLAQRFLIKPEKPGVSFYTVQAFLEGEQGYDSKPGRTAEATLANNRRLATVDRGG
                     GPYHVLYVSGRPNWEFKFLSRAVAEDDEMRLMGLIRIAKKEPKFTFKGRQGERTNPLF
                     RGFGNNSDPTAEQYDQPVLLRVPPTKEEEQDLKGGFPKDAEDLYRYHAVILDDVEAAF
                     FTQDQLSLLQKFVSQRGGGLLMLGGPHSFAEGGYQRTPVGEMLPVYLDRNIGASPPDG
                     FRLKLTREGWLQPWVRVRANEGDEEKRLAAMPPFKTVNRIDAIKPGATVLSQVETSDG
                     AARPALVVQPFGRGRTAALLVGDMWRWNIRREDYKESDLDKSWRQTVRWLVADVPQPV
                     EVETRRSSGTALPGVELVVRARDKQFSPLDNATVAIKVQTPDKRSIDLVAESSAKSPG
                     EYVATFAPREAGAYRASISVTAADGSDVGARATGWSVEPETEEFRTLSVNRQLLERMA
                     KETGGEIVSADGLDGFVSSLPNRKIPKVESWTYPLWHQWQMFLAAIVCLVGEWGLRRW
                     RGLP"
     gene            2060..3070
                     /locus_tag="VFB80_22705"
     CDS             2060..3070
                     /locus_tag="VFB80_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36665.1"
                     /translation="MRFQISDFRFPIGLVWVTLACVGWAVPAVCRADEPKAAVIVVVG
                     AEGEAQFGQQFRTWALRWEAAAKAAGAELTSIGLAESGAKSDREILSDRLAELAKAPS
                     TEAAWLVLIGHGTFDGKTARFNLRGADLTPADLAGWLKPLERPLAIINCTSGSGPFLN
                     ELSGANRTVIVATKSGHEYNFARFGDYLSSAIADLKADLDKDDQTSLLEAFLMAAGGV
                     NEFYKTEARLATEHALLDDNGDKLGTPVEFFQGVRAVKSAKSGAAPDGLVAAQLVLVR
                     SQNEEKLPAATRARRDELERDLAELRLKKAKLSEAEYFGLLEPLLVELARLYESADAK
                     QH"
     gene            complement(3089..3514)
                     /locus_tag="VFB80_22710"
     CDS             complement(3089..3514)
                     /locus_tag="VFB80_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36666.1"
                     /translation="MNVRRLLVTIVLAAGAAASAAAQDKVVIPVPCPHEQIAYHDVVT
                     HRCKLVPDKKQIKKTVYEVQEVPFCLPKLPPLLSLLHHGCCDECVECDCPRYKRVLLK
                     KEIVCEEVCVMKCVVEEVVERVPCRVCQGPCPQCAAAAK"
     gene            complement(3591..>4981)
                     /locus_tag="VFB80_22715"
     CDS             complement(3591..>4981)
                     /locus_tag="VFB80_22715"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN36667.1"
                     /translation="GSPAGYETSAAVVEAAGANLAPPSDPEWIQVGQDLSMAARWNNG
                     LEVATKDKAFRIHVGGRWQLDTSWFSADQAVQQNLPGNVMYHDGIDFRRARLRIDGTM
                     YEVIEFACEYDFVNGLRVGHPNNSDFLDQNVPALTDLWLQYSHTPLGNIRIGNQKEAI
                     GFEHIVSSRFLPFMERSYNQDTFYGGAFNGFIPGASINGNVWEDRASYHLGLYKPTNN
                     VFGFSTNDGDYSVTGRLTWLPIYEQEGRQLLHLGASVRQATTYDDRLRFRTRDAIRAG
                     VATQWPVPADITVFGDTVQWFNFELAAVQGPWTLQAEWLLSNTHDAQRVGGGPLVQDL
                     LYQGGYVQLLCFLTGESDTYSLERMAFDRMKPFENAFWLRDANCESIFGRGAWQVGAR
                     YNFLDLNDQGINGGQLHNFTAGLNWFWNPNSKWQFNYFATYRDVSQASDANIADGSGW
                     IHGWGMRFAQDF"
BASE COUNT          855 a         1631 c         1672 g          823 t
ORIGIN      
        1 ggcaattgtt tcaggcgcgg ctggccgaaa agtcatcctg gctgacgcgg gtggctcagg
       61 actttgacct gcggcggtac gccttcgaca cgacgctgcg tccgatcaag ggctttgagg
      121 agctgacgct cgatggccag gcctccgcga tgcagggctc actcaccgcg ctggccgaac
      181 gcttccgcgg tcagcccatc gccggcatcc tgctgttcac cgacggcaat gccacggacc
      241 tggcgggcaa tcttgactgg tcgcgcctgc cgccggtcta tccggtgctg ccctcggcga
      301 gcggcgagct ggtcgatctg tcgctggccc gcgtcagcat cagccagacc aacttcgagg
      361 ccgccccggt cacgatcacg gccgagatcg acggccagtc ggtcgccggc cagaaaatcg
      421 tcatccgtgt cctcgacgaa gagaacaaag aggtcgagcg ccgcacgctc gccgcgccgg
      481 cgggcacgga gccgctggct cagcgattcc tcatcaagcc cgagaagccc ggcgtcagct
      541 tctacacggt gcaggcgttt ctggagggtg agcaaggata cgacagcaag cccggccgca
      601 ccgccgaggc cacgctggcg aacaatcgcc gcctggccac cgtcgatcgc ggcggcgggc
      661 cctaccacgt gctgtacgtc tccggccggc ccaactggga attcaaattc ctcagccggg
      721 ccgtcgccga ggacgacgag atgcgcctga tgggcctgat ccgcatcgcc aagaaagagc
      781 ccaagttcac cttcaaaggc cggcagggcg agcggaccaa cccgctgttt cgcggcttcg
      841 gcaacaattc cgatcccacg gccgagcagt acgaccagcc ggtgctgctc cgcgtgccgc
      901 cgacgaaaga ggaagagcag gacctcaagg gcggcttccc gaaggacgcc gaggacctgt
      961 accgctatca cgcggtgatc ctggacgacg tcgaggccgc cttcttcacg caggaccagc
     1021 tgtcgctgct gcagaagttc gtcagccagc ggggcggcgg cctgttgatg ctcggcggac
     1081 cgcattcctt cgctgaaggc ggctatcagc ggacgcccgt cggcgaaatg ctgccggtct
     1141 atctcgaccg caacattggg gccagcccgc cggacggctt ccgcctcaag ctgacccgcg
     1201 agggctggtt gcagccgtgg gtccgcgtac gggccaacga gggcgacgaa gagaagcggc
     1261 tggcggcgat gccgcccttc aagacggtca accggatcga cgcgatcaag ccgggggcga
     1321 ccgtgctgtc gcaggtcgag acgtccgatg gcgcggcgcg gccggccttg gtggtgcagc
     1381 cgttcggccg cggccgcacg gcggcgctgc tcgtggggga tatgtggcgg tggaatatcc
     1441 gccgcgagga ttacaaggag agcgatctcg acaaatcctg gcggcaaacc gtccgctggc
     1501 tggtggccga cgtgccgcag ccggtggagg tcgagacgcg gcggtccagc ggcacggcgt
     1561 tgccgggcgt cgagcttgtc gtgcgggccc gcgacaagca gttctcgccg ctcgataacg
     1621 ccacggtcgc catcaaggtg cagacgcccg acaagcggag catcgacctg gtggccgaaa
     1681 gcagcgcgaa gtcgcccggt gaatacgtcg ccacgttcgc gccgcgcgaa gccggagcgt
     1741 accgcgcgag catttcggtg accgccgccg acggcagcga cgtgggcgct cgggccacgg
     1801 gctggtcggt cgagcccgag acggaagagt tccgcacgct gtcggtgaac cggcagctct
     1861 tggagcggat ggccaaggag acgggcggcg agatcgtgag cgccgacggc ctggacggct
     1921 tcgtcagcag cttgcccaat cggaagattc ccaaggtcga gagctggacc tacccgctgt
     1981 ggcatcagtg gcagatgttc ctggccgcga tcgtgtgcct ggtgggcgag tggggcctgc
     2041 ggcggtggag gggattgccg tgagatttca gatttcagat ttcagatttc cgattggtct
     2101 ggtgtgggtg acgctggcct gcgtagggtg ggctgtgccc gccgtctgcc gtgccgatga
     2161 accgaaagcg gcagtgatcg tcgtcgtcgg cgccgaaggc gaggcgcagt ttgggcagca
     2221 gttccgcacg tgggctctgc ggtgggaggc ggcggcgaag gccgccgggg cggagctgac
     2281 ctcgatcggc ctggcagaaa gcggcgcgaa gagcgaccgc gagatcctct cggaccggct
     2341 ggcagagctg gccaaagcac cctccaccga ggccgcgtgg ctggtgctga tcggccacgg
     2401 cacgttcgat ggcaagacgg cgcggttcaa cctccgcggc gcggatctca cgcccgccga
     2461 cctggccggc tggctcaagc cgctcgagcg gccgctggcg atcatcaact gcacttccgg
     2521 cagcggcccg tttttgaacg agctgtcggg agcgaaccgc accgtgatcg tcgccaccaa
     2581 gagcgggcac gagtacaact tcgcccgttt cggcgattat ctgtcgtccg cgatcgccga
     2641 tctcaaagct gatctcgaca aggacgacca gacgtcgctc ctggaagctt tcctgatggc
     2701 cgccggcggc gtgaacgagt tctacaagac cgaggcccgc ctggcgacgg agcacgcact
     2761 cttggacgac aacggcgaca agctcggcac gcccgtcgaa ttctttcagg gcgtgcgggc
     2821 cgtcaagagt gccaagagcg gcgcagcgcc cgacggcctg gtcgccgcgc agctcgtcct
     2881 cgtccgcagc cagaacgagg aaaagctgcc cgcggccacc cgcgcccgcc gcgacgaact
     2941 ggagcgcgac ctggcggagc tgcgcctcaa gaaggcgaag ctctcggaag ccgagtattt
     3001 tggcctcctg gagccgcttc tcgttgagct ggccaggctc tacgagtcgg cggacgcaaa
     3061 gcagcactag tcgcccgccg cggccgagtt acttcgccgc cgcggcgcac tgcgggcagg
     3121 gcccctggca gacgcggcag ggaacgcgct caacgacctc ctcgactacg cacttcatga
     3181 cgcagacctc ctcgcagacg atctcctttt tcaggagcac gcgcttgtag cgcgggcagt
     3241 cgcactcgac gcactcgtcg cagcagccgt gatgcagcag cgacaacagc ggcggcagct
     3301 tcggcaggca gaagggcacc tcctgcactt catacaccgt cttcttgatc tgcttcttgt
     3361 cgggcacgag cttgcagcgg tgcgtaacca cgtcgtgata cgcaatctgc tcgtgcgggc
     3421 aggggacggg gatgacgacc ttgtcttgcg cggcggcgct ggcagcagcg ccggcagcaa
     3481 gaacgatcgt gaccaacaac cggcgtacgt tcatggggag actccttttc gatttgcgcc
     3541 ggcgcgttca tgcagccgcc gtcgcctccg gctccgggtt aaacgagttg tcaaaaatcc
     3601 tgggcgaacc gcatccccca gccgtgaatc cagccgctgc cgtcggcgat gttcgcgtcg
     3661 ctggcctggc tgacgtcgcg gtaggtggcg aagtagttga actgccactt gctgttggga
     3721 ttccagaacc agttcaggcc ggcggtgaag ttgtgcagtt gcccgccgtt gatgccctgg
     3781 tcgttcaggt cgaggaagtt gtagcgggcg cccacttgcc acgccccgcg gccgaagatg
     3841 ctctcgcagt tcgcgtcgcg cagccagaac gcgttttcaa acggcttcat ccggtcgaaa
     3901 gccatccgtt ccaggctgta ggtgtcgctc tcccccgtca ggaaacagag cagctgcacg
     3961 tagccgccct gatacagcag gtcctgcacg agcgggccgc cgccgactcg ctgggcgtcg
     4021 tgcgtgttgg agagcagcca ctcggcctgc agggtccacg gcccctgcac cgcggccagc
     4081 tcgaagttga accactgcac ggtgtcgccg aagacggtga tgtccgcggg cacgggccac
     4141 tgtgtcgcga cgcccgcccg gatcgcgtcg cgggtgcgga accgcagccg gtcgtcgtag
     4201 gtggtcgcct gccgcaccga ggcgcccagg tgcaacagct gccgtccttc ctgctcatag
     4261 atcggcagcc acgtcagccg cccggtgacc gagtagtcgc cgtcgttggt gctgaagcca
     4321 aaaacgttgt tcgtcggctt gtacaggccc aggtgatagc tcgcccggtc ctcccagacg
     4381 ttgccgttaa tcgacgctcc cggaatgaag ccgttgaacg ccccgccata aaacgtgtcc
     4441 tggttgtacg accgttccat gaacggcaga aaccggctgc tcacgatgtg ctcaaagccg
     4501 atcgcttcct tctgattgcc gatgcgaata ttgcccagcg gcgtgtgcga gtactgcagc
     4561 cacaggtcgg tgagggcggg aacgttctgg tcgaggaagt cggagttgtt cgggtgccca
     4621 acccgcagac cgttgacgaa gtcgtactcg caagcgaact cgatcacttc gtacattgtg
     4681 ccgtcgatcc gcaagcgggc ccggcgaaaa tcgatgccgt cgtgatacat cacattgccc
     4741 ggcaggttct gctgcacagc ctggtcggcg ctgaaccacg acgtgtcgag ctgccaccgc
     4801 ccgccgacgt gaatgcgaaa tgccttgtct ttcgtggcga cctccaatcc gttgttccag
     4861 cgggccgcca tcgacaggtc ctgtccgacc tggatccatt ccggatccga gggcggtgcg
     4921 aggttcgcgc cggccgcctc gaccaccgcc gccgacgtct cgtagcccgc gggcgatccg
     4981 c
//
LOCUS       DASRJB010000866         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_815987, whole genome shotgun sequence.
ACCESSION   DASRJB010000866 DASRJB010000000
VERSION     DASRJB010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815987"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1647
                     /locus_tag="VFB80_22720"
     CDS             397..1647
                     /locus_tag="VFB80_22720"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36668.1"
                     /translation="MSPAAVSLALVVASLGQYSNRYNSYGTIKKDELEYQQQNFKQWW
                     GQDLVIKLEELPLEGRVPDFRVPYSGHDYPDQNGGTINALVKYDRAFHGGRMLATEFE
                     RQDIGGHRGGGERRGLFGRIRRGGTPGWYGHCNGWTAASIRHAEPQHNVVRNGVTFTP
                     ADIKGLLAEMYMYTESEFLGGVDPAINAGTLHLTLTNWLGRGSHPVGMETAIGEVVIN
                     YPIFSYKAKVNKLSDRQSEVQMSIRYAVNTPRETDKGPKDFNRLMYFHYSLDTNEAGE
                     IQAGRYYGDSNQIDMLWSPLQPAQGGEEKNKRGNPHMSIKEVLAIWRESVPEDLRKKW
                     LNIDPTEEDRVLPPEDVAATTTAEPPAPATTAAPAEGTAPATAAPAGSPSPAGSTPPS
                     TSPATPTSPATPAAPAPAETPANP"
     gene            1869..2810
                     /locus_tag="VFB80_22725"
     CDS             1869..2810
                     /locus_tag="VFB80_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN36669.1"
                     /translation="MPAHSPAPEPPAPKNPALPYVWMLLGAAAFAVMSILTARLKDRV
                     DWQWIAIARAGLAMVFAATLAKAGGAKLVLLRPRKLWMRSLAGSISLVCGFYAMTHYP
                     VSEVLTLTNMFPIWVALLSLPLLGEWPPFDIWPALAMGVIGVVLIQSPQIPGGHLAVA
                     AALVSSFTSAVALIGLHQVRELDARSVVAHFSGVALACCVGALFVFPRRHSGLDLTLN
                     TGLTLLGIGVCATCGQLLLTKAFAAGKPSRVSVVGLSQVGFTMLLEIAIDGRPFSPWT
                     LLGIGLVVAPTAWTLLRGAARSRVVEAEPPLPLKKAA"
     gene            2833..3180
                     /locus_tag="VFB80_22730"
     CDS             2833..3180
                     /locus_tag="VFB80_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZN36670.1"
                     /translation="MRMVLAFIQPTKLKAVQEALARIGVDRMTVCDALGYGQQRGQAA
                     GRSRESSVKLLRKVALEISVNEDFLDKTVETIERVARTGESGAIGDGKIFVLPVEEVV
                     RISDTVRGPEAVS"
     gene            complement(3243..3728)
                     /locus_tag="VFB80_22735"
     CDS             complement(3243..3728)
                     /locus_tag="VFB80_22735"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZN36671.1"
                     /translation="MKYEPLLHVVLYQPEIPPNTGNIGRSCVAVGAKLWLIKPLGFRI
                     DEKELRRAGLDYWPHLDWEAVEDWAGLAAKLAGPLAAGRAWFFTKTAPRLYTDASFQK
                     GDLLVFGNESGGLPPELLATHRERTLRLPMRKQVRSLNLSATAAAAMYEAVRQLGVVP
                     E"
     gene            3809..>4558
                     /locus_tag="VFB80_22740"
     CDS             3809..>4558
                     /locus_tag="VFB80_22740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN36672.1"
                     /translation="MTAAPSNGRCSSCDAALAPQARYCGQCGTKVGEGVAATMSYTSL
                     RPPWEPGRSGAERFAAGSVLAGRFRIVTALGQGGMGEVFRADDLSLGQSVALKFLPPA
                     LAHDIDRLQRLRGEVKNARQVAHPNVCRVYDLGEADGQHFISMEYVDGHDLGALLKQI
                     SRMPAERGVEIARQLCLGLGAIHDRGLLHRDLKPANVMLDGRGQVRITDFGLAAPAEK
                     LPAGEIRHGTPAYQAPEQLAGREVTARSDIYA"
BASE COUNT          750 a         1503 c         1534 g          771 t
ORIGIN      
        1 caggctcccg cagcggtgca ggccccgatg gccccggtcc agaagccggt gcaggccccg
       61 atccagaagc actcggtcgc ccaggtgcag tcgccggtcc agaagggtgc tgctcccgtg
      121 ccggtcgctc cggtgaagac cgcccgcgtc ggcatgttcg gccgctatcg cggctagtgc
      181 gtgcgtagag aagtatgacg agtccgctgc tcgtgcgcgg acttgagttc cgtttgggtc
      241 ctgacggaat tgctgagcgt agggcgagcg gtgctcggtc ctgccgtatg attccagccg
      301 gacgaatttg agcgccctcg gattggcatt tcgtccgagg gctttttcgt cggctatgat
      361 tgctgcatct tcagtggctc accaggaatc tctgccatgt cccccgccgc cgtttcgcta
      421 gcccttgttg tggcttcgct cggtcagtac tccaatcgct acaactcgta cggcacgatc
      481 aagaaggacg agctggagta tcagcagcag aacttcaagc agtggtgggg ccaggacctg
      541 gtgatcaagc tcgaggagct gccgctggaa ggccgcgttc ccgacttccg cgttccgtat
      601 tcgggccacg actatccgga tcagaacggc ggcacgatca acgcgctcgt caagtacgac
      661 cgcgcattcc acggcggccg gatgctggcc accgagttcg agcggcagga catcggcggg
      721 catcgcggag gcggcgaaag gcgcgggctc ttcggacgca ttcgccgtgg cggcacgccg
      781 ggctggtacg gccactgcaa cggctggacg gcggcctcga tccgccacgc ggagccgcag
      841 cacaacgtgg tccgcaacgg cgtgacgttt acgcccgcgg acatcaaggg gctgctcgcg
      901 gaaatgtata tgtacacgga gtcggagttt ctggggggcg tcgatccggc gatcaacgcg
      961 gggacgctgc acctgacgct gaccaactgg ctgggccgcg gctcgcatcc cgtcggcatg
     1021 gagacggcaa tcggcgaagt cgtcatcaac tacccgatct tctcgtacaa ggccaaggtc
     1081 aacaaacttt cggaccggca gtcggaagtg cagatgtcga tccggtacgc agtaaatacg
     1141 ccgcgggaga cggacaaggg cccgaaggat ttcaaccggc tgatgtattt ccactactcc
     1201 ctcgacacga acgaggcggg cgagattcag gccggccggt attacggcga cagcaaccag
     1261 atcgacatgc tctggtcgcc gctgcagccg gcgcagggcg gcgaagaaaa gaacaagcgc
     1321 ggcaatccgc acatgagcat caaggaggtg ctggcgatct ggcgcgagtc ggtgcccgag
     1381 gacctgcgga agaagtggct gaacatcgat ccgaccgaag aggaccgcgt gctgccgccg
     1441 gaggacgtgg cggcgacgac cacggccgag ccgcccgcgc ccgcgacgac tgctgcgccg
     1501 gcggagggga ccgctccggc gaccgctgcg cctgccggca gcccatctcc ggcgggcagt
     1561 acgcccccgt ccacgtcgcc ggcgacgccc acttcgccag cgacgccagc agcgccagcg
     1621 cccgccgaaa cgccggcgaa tccctagctg gaacagtgca aattgcaaag tgcaaagtgc
     1681 aaagtgcaaa ttgaacgaag aagacacttt gcaatttgca tttctcactt tgcaatttgc
     1741 aatgctgccg ggccctctcc ctaacgctgt cccaacgggc cctgcgaacc tttgccgccg
     1801 gcgaagtgtc gtcggtcttc attccgcgga cttacgtgcg tacacttagg ctttgcccgc
     1861 ccgaaccgat gcccgcccac agcccagcac ccgagccgcc cgccccgaag aatcccgcgc
     1921 tgccctatgt gtggatgctg ttgggagcgg cggcgtttgc cgtgatgtcg atcctgacgg
     1981 cccgcctgaa ggaccgcgtc gattggcagt ggatcgcgat tgcccgcgcc ggtttagcga
     2041 tggtctttgc cgccacgctg gcgaaggccg gcggcgcgaa gctcgtcctg ctgcggccgc
     2101 ggaagctctg gatgcggagc cttgccggca gcatcagcct ggtctgcggc ttctatgcga
     2161 tgacgcacta tcccgtgtcg gaggtgctca cgctgacgaa catgttcccg atctgggtcg
     2221 ccctgctgtc gctgccgctc ctcggcgagt ggccgccatt cgacatctgg ccggccctgg
     2281 cgatgggcgt gatcggcgtg gtgctgatcc agtcgccgca gattcccggc ggccacctgg
     2341 cggtggcggc ggcgctggtc agctcgttca ccagcgcggt cgcgctgatc gggctgcacc
     2401 aggtgcgcga actcgacgcg cggtcggtcg tcgcgcactt ttcgggcgtg gccctggcct
     2461 gctgcgtggg ggcgctgttc gttttcccgc ggcggcacag cggcctggac ctgacgctca
     2521 atacggggct gacgctgctg ggcatcgggg tctgtgcgac ctgcgggcag ttgctgctca
     2581 ccaaggcctt tgcggcgggc aagccgtcgc gggtctcggt cgtcggcctg tcgcaagtcg
     2641 gcttcacgat gctgctggag atcgcgatcg acgggcggcc gttcagtccc tggacgctgc
     2701 tgggcattgg cctggtggtc gcgcccacag cctggacgct gctccgcggc gctgcccgct
     2761 cgcgcgtcgt ggaagcagag ccgccgctgc cgctgaagaa ggccgcctga cccgttacgc
     2821 aggagcgccg cgatgcggat ggtgctcgcc ttcatccaac cgacgaagct caaggccgtg
     2881 caggaggctc tcgctcggat cggcgtggat cggatgacgg tctgcgacgc gctgggctat
     2941 ggccagcagc gcggtcaggc cgccggccgc agccgcgaat cgagcgtgaa gctgctgcgg
     3001 aaggtggccc tggagatttc ggtgaacgag gactttttgg acaagacggt cgagacgatc
     3061 gagcgcgtcg cccgtaccgg cgaaagcggc gcgatcggcg acggcaagat tttcgtactg
     3121 cccgtcgagg aggtcgtccg catttcggac acggtccgcg ggcccgaagc ggtgtcgtga
     3181 atgcggaatt cggatacgga atggagcaca gagtatcaat tccgcactcc gcgctcccgc
     3241 atttactccg gcacgacgcc caactgccgc acggcctcgt acatcgccgc cgccgcggtc
     3301 gccgagaggt tcaggctgcg gacctgcttc cgcatcggca ggcgcagcgt ccgctcgcgg
     3361 tgagtggcca atagctccgg cggcaggccg cccgactcgt tgccgaaaac cagcaggtcc
     3421 cctttttgaa aggaggcgtc ggtgtagagc cgcggggcgg tcttggtgaa gaaccaggct
     3481 cggccggcgg ccagcggccc agcgagcttc gccgccagcc ccgcccaatc ttcgaccgct
     3541 tcccagtcca ggtgcggcca gtagtcgagc ccggcccgcc gcagctcttt ctcgtcgatg
     3601 cgaaagccca gcggcttgat cagccacagc ttcgcgccca ccgccacgca gctccgcccg
     3661 atgttgcccg tattcggcgg aatctccggc tgatagagga cgacatgcag cagcggttcg
     3721 tatttcacgg ctgacgatag taagcggcca tcggccgagc ctagaataac gccacacctt
     3781 aatcctagcg tgggtcgttc gatctcttgt gaccgccgcg ccttccaacg gtcgttgctc
     3841 gtcctgcgat gccgcactag cgccgcaggc tcgctattgc gggcagtgcg gcacgaaagt
     3901 cggcgaaggc gtcgcggcga cgatgtcgta caccagcctg cgcccgcctt gggaaccggg
     3961 ccgaagcggc gccgagcgtt tcgcggcggg gtcggtgctg gccgggcgtt tccgcatcgt
     4021 cacggcgctg ggccagggcg gcatgggcga agtgttccgc gccgacgacc tttcgctcgg
     4081 ccagtcggtg gcgctcaagt tcctgccgcc ggcgctggcg cacgacatcg accgcttgca
     4141 gcggctgcgc ggtgaggtga agaacgcccg tcaggtcgcc catcccaacg tctgccgcgt
     4201 gtacgacctg ggcgaagccg acggccagca tttcataagc atggagtacg tggacggcca
     4261 cgacctgggc gcgctgctca agcagatcag ccggatgccg gcagagcgcg gcgtcgagat
     4321 cgcgcggcag ctctgtctcg gtctgggcgc gatccacgac cggggcttgc tacatcgcga
     4381 tctcaagccg gccaacgtca tgctcgacgg ccgcgggcaa gtgcggatca ccgacttcgg
     4441 gctggccgcg cccgccgaga agctgcccgc cggcgaaatt cgccacggca cgcccgccta
     4501 tcaagcgccc gagcagctcg ctgggcgcga agtcaccgcc cgcagcgaca tctatgcg
//
LOCUS       DASRJB010000867         5772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3360602, whole genome shotgun sequence.
ACCESSION   DASRJB010000867 DASRJB010000000
VERSION     DASRJB010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360602"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFB80_22745"
     CDS             <1..638
                     /locus_tag="VFB80_22745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN36673.1"
                     /translation="SRLMVFSGQRCDPLTGLCNRQALDEAMENYLTMHRRYGMLFSVA
                     VFDLDNFKQFNATHGHAHGDRLLKQVASLLDEQARETDIVTRSGGEEFVVVLPATELP
                     GATTFAERLRQAIEQKTSLTISAGVAMVGDGDDAKTFLTRTDTALYAAKAAGRNCVFI
                     HTGKDTLPAESQDLMAPAPTETRASDKAAPPKAAPAKPAPPQPAASASKPA"
     gene            complement(640..891)
                     /locus_tag="VFB80_22750"
     CDS             complement(640..891)
                     /locus_tag="VFB80_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane or secreted protein"
                     /protein_id="HZN36674.1"
                     /translation="MRHCRFLLLMFAAAAGGCAGGPNYDFWHPGNIHTQRLRAVVHDP
                     YPDQDLAPVVMGGRPRDYSEPLPEAVRNRLYTDSFYGRP"
     gene            complement(1056..2093)
                     /locus_tag="VFB80_22755"
     CDS             complement(1056..2093)
                     /locus_tag="VFB80_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZN36675.1"
                     /translation="MADPLYPLKFQPLFKRYLWGGRRLGSVLGKPIGEGGDYAESWEV
                     ADHPQGQSVIANGPLAGKPLHDLVVQQPDDMFGRHAEQCQRLGRFPLIFKYLDAQQDL
                     SVQVHPDDAAAARLSPPDLGKTEAWLILDAQSGSRVYAGLKIGFGPEALRREVERGRT
                     ELCLHSFEARPGQCLFLPAGTVHALGAGSLVAEIQQASDTTYRLFDWNRTGPDGQPRQ
                     LHVEEALAAIDYSRRAVLPTPPQRTERPFVERLVACDKFVLDRWTLSEMHKLGGDRRF
                     HILSVLEGEMLLSGSAPQVESGRRLGASTSDEVSLLSLIRGQTVLVPACLGEITVAAR
                     DAAVMLDVYLP"
     gene            2187..2543
                     /locus_tag="VFB80_22760"
     CDS             2187..2543
                     /locus_tag="VFB80_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36676.1"
                     /translation="MPKKKSAKKPTKGTRKPAKKSPAKKRAPKKKVSLGRPTVTVEEK
                     LFMLFHGDYEARQVFEFLRAETVGELEQFSPPEIIHRLSRPIRETVDRIRIALAQKNR
                     CLAGDEEFAAQHRPSK"
     gene            complement(2544..4235)
                     /locus_tag="VFB80_22765"
     CDS             complement(2544..4235)
                     /locus_tag="VFB80_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HZN36677.1"
                     /translation="MSPAYSAPPKVTALFPAGGQRGQTVEVTASGDFSTWPVQVWADR
                     PGLTATAEKDKGKLKVEIAAEAAAGTYWLRLCNADGASVLRPFIVGTLAEVLESETND
                     LPDKPQTVEPRVVVNGKLAKSGDVDGYRLELKQGQTLVASLVANSTLGAPMDAALQIC
                     ELVDKASARREPLDNTRVSAADNQREAGASRSPGKAEAFVVAQNHDSAGLDPLLAFTA
                     PRDGAYLVRLFALPAQPDSSVRFAGGDDYLYRLTLTTGPYIDHALPLAAENTETQVQF
                     GGWNLGEVRSTIVPPLSAEPDPLPDGGLAWAWHPSAAGAFPLPRLANTIGGRLEGLGA
                     IHTHSFPGSKGQKVRLRAAAKSLGFPTDPVIAVLDSAGKVLAEADDSGRDERDATLEF
                     TPDADGTYQVQIRDLARRGDVRMAYRLTIEPVQPDFSLSLAADSFVLEKDKPLEIPVT
                     VAARDGFRDAVEVRAVGLPAGITAEPVKFEPTGDAPMSKSGGGRRGKSKSGTAPSGPT
                     AKLILKADPATAQAGGFAFRIEGRTSGDSPLIRTARFPLNLPLAGQHHAAWLTVK"
     gene            complement(4352..5674)
                     /locus_tag="VFB80_22770"
     CDS             complement(4352..5674)
                     /locus_tag="VFB80_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36678.1"
                     /translation="MTTHRNCEGFTRRDCLQLGLGGLLGGGLVGALRATASAAVNPAR
                     QAKSCILIWMDGGPSHYETFDPKPDAPIEFRGQFDAIPTKVAGVSFSQHMKRLASIAD
                     KFAVIRSIRHDQGNHGAGNHYMMTGAPPRIPVGCGAFVSYHPSLGSAAARECPVPPGL
                     PGYFSLGQMSRSGGPNFLGAQYAPFVVEESPDKPDFRVRDVAIPRDLSEGRFADRKDL
                     RKLVDRMVRINEEAAADPVRDVDRFYEQGYSLVSSKEAQQAFDIHREDPKLREKYGRN
                     NLGQRCLLARRLVEAGVPFITVYDGGWDHHTDLFNSFTKRMPGWDNSVATLIEDLSDR
                     GLLDSTLVIALGEFGRTPQINKDAGRDHWSNAMSVLFAGGQAPGGQAIGATDSKGFAA
                     IDRVLSPENFVSTIYTKLGLDPDKMYYTHEGRPVHLVSDPTPIRELMG"
BASE COUNT          974 a         1969 c         1927 g          902 t
ORIGIN      
        1 gcagccggct gatggtcttc agcggccagc ggtgcgatcc gctgacgggc ctctgcaacc
       61 ggcaggccct ggacgaggcg atggagaact acctgacgat gcaccgccgc tacgggatgc
      121 tgttctcggt ggcggtcttc gacctcgaca atttcaagca gttcaacgcg acgcacggcc
      181 acgcccatgg cgaccgactg ctcaagcagg ttgcgtcgct cctggacgag caggcccgcg
      241 agaccgacat cgtcacccgc tccggcggtg aggagttcgt cgtggtgctg ccggccaccg
      301 agctgccggg ggccacgaca tttgccgagc ggctgcgtca ggcgatcgag cagaagacca
      361 gcctcacgat cagcgccggc gtggcgatgg tcggcgacgg cgacgacgcc aagaccttcc
      421 tcacgcggac cgacaccgcg ctctacgccg ccaaggccgc cggccgcaac tgcgtcttca
      481 tccacaccgg caaggacact ctgccggccg agagccagga cctcatggcg cccgcgccga
      541 cggagacgag agcttccgat aaagcagcgc ctccaaaggc cgctcctgcc aagcccgccc
      601 cgccccagcc cgcggccagc gcgagcaagc cggcctagcc tacggccgcc cgtaaaagct
      661 gtcggtgtag agccggttgc gaaccgcctc gggcagcggc tcggaataat cccgcggccg
      721 accgcccatc accaccggag ccaagtcctg gtccgggtac ggatcgtgaa cgaccgcccg
      781 cagccgctgg gtgtggatgt tgccaggatg ccagaagtcg taattcggcc cgcccgcgca
      841 gccgccagcc gcagcggcga acatcagcaa taggaagcgg cagtgtctca ttggcgaatc
      901 ccagcggccg gatgatacgg agccgccatt cgcggctcaa gcgagaatct gacagcacca
      961 gcgcgccagc acgccgtaat gctcggaggc gatccaacta cgagcgcgac gcgcaacggg
     1021 ttcgaatcgc cactcacttc tcaccactca cccgcctaag gcaaatacac atccagcatc
     1081 accgccgcgt cgcgggccgc caccgtgatc tcgccgagac acgccggaac gaggaccgtc
     1141 tggccgcgaa tcagcgacag cagtgagacc tcgtccgaag tgctggcacc cagccggcgg
     1201 cccgactcga cctgcggcgc ggagccgctg agcagcattt ccccctcgag cacgctcagg
     1261 atgtgaaacc gccggtctcc gcctagcttg tgcatttcgg aaagcgtcca gcggtccaga
     1321 acgaacttgt cgcacgcaac caggcgttcg acgaacggcc gctcggttcg ttgcggaggc
     1381 gttggcagga ccgcgcgccg cgaatagtcg atcgcggcca gtgcttcctc gacgtgcagc
     1441 tggcgtggct gcccatccgg tcccgtgcga ttccagtcga acaggcggta cgtggtatcg
     1501 ctggcctgtt ggatctccgc cacgagtgag cctgcgccga gggcgtgcac ggtgcccgcg
     1561 gggaggaaca ggcactgccc cgggcgggcc tcgaacgagt gcaggcacaa ctccgtgcgg
     1621 ccccgttcca cttcgcggcg cagcgcttcc ggtccaaagc cgattttcag cccggcatag
     1681 acgcggctgc cgctctgcgc gtcaagaatc agccaggcct cggtcttgcc caggtcgggc
     1741 ggcgagagcc gcgccgcggc tgcgtcgtcc ggatgcacct gcaccgagag gtcctgctga
     1801 gcgtcgagat atttgaagat cagcggaaac cggcccagcc gctggcactg ctctgcgtgc
     1861 cggccgaaca tgtcgtccgg ctgctgcacc acgagatcgt gcaagggctt gccggcgagc
     1921 ggcccgttgg cgatgaccga ctgcccctgc ggatgatcgg ccacttccca actctcggca
     1981 taatcgccgc cttcgccgat gggcttgccc agcaccgagc ccaatcgccg gccgccccac
     2041 aggtagcgct tgaagagcgg ctggaacttg agcggataga gcgggtcggc catgaagcag
     2101 ctccgcgagc agttgacgag aacaggcgat cgccggccga gtgtgaacta taatcggatt
     2161 ttccctgccc cgcctgagcc tgcatcatgc ccaagaagaa atccgcaaag aagcccacca
     2221 aggggaccag gaagcccgcc aagaagtcgc cggcaaagaa gcgggccccg aagaagaaag
     2281 tctcgctcgg ccgacccacg gtcaccgtgg aggagaagct gttcatgctc ttccacggcg
     2341 attacgaagc ccggcaggtg ttcgagtttc tgcgggccga gaccgtcggc gagctggagc
     2401 agttctcgcc gccagagatc atccaccgcc tctcgcggcc gatccgcgag acggtagatc
     2461 gcatccgcat cgcgctcgcc cagaagaatc gttgcctggc gggcgacgaa gagttcgccg
     2521 cccagcacag gccatccaaa tagctatttc accgtcagcc acgccgcgtg atgctggcct
     2581 gccagcggca ggttgagcgg gaagcgggca gtgcgaatca gtggcgagtc gccgctcgtg
     2641 cggccttcga tgcggaaggc gaaaccgccc gcctgggccg tcgccggatc ggctttgaga
     2701 atcagcttcg ccgtcgggcc gctgggcgcc gtaccgcttt tgctcttccc gcggcggccg
     2761 ccgcccgact tgctcatggg cgcgtcgccg gtcggctcga acttcaccgg ttcggccgtg
     2821 attcccgccg gcaggccgac ggcgcgaacc tcgactgcat cgcggaatcc gtctcgcgcg
     2881 gcaactgtca ccggaatctc cagcggcttg tctttttcca ggacgaacga gtcggccgcc
     2941 agcgacaggg aaaaatccgg ctgcaccggc tcgatggtca ggcgataggc catccgcacg
     3001 tcgccgcggc gagccaggtc gcggatttgc acctggtaag tgccgtctgc atccggagtg
     3061 aactccagcg tggcgtcgcg ttcgtcgcgg ccgctgtcgt cggcctcggc cagcactttg
     3121 cccgcgctgt cgagcacggc gatcaccgga tctgttggaa atccgagcga tttggcggcc
     3181 gctcgcaggc ggaccttctg gcccttcgag ccgggaaatg agtgcgtgtg aatggcgccc
     3241 agcccttcca gccggccgcc gatcgtgttc gcgaggcggg gcagcgggaa ggcgccggcg
     3301 gcgctcggat gccaggccca agccagcccg ccatcgggca gcgggtcggg ctcggccgaa
     3361 agcggcggca cgatcgtgga gcggacttca cccagattcc agccgccgaa ctgcacctgc
     3421 gtctccgtat tctccgcggc cagcggcagg gcgtggtcga tgtacggccc ggtggtcagt
     3481 gtcagccggt agagatagtc gtcgccgccc gcgaaccgca cgctgctgtc gggctgtgcc
     3541 gggagggcga atagccgcac caggtacgcc ccgtcccgcg gcgcggtgaa cgcgagcagc
     3601 gggtcgaggc cggcggagtc gtggttctgg gcgacgacaa acgcctcggc cttgcctggc
     3661 gagcgggacg cgccagcttc ccgctgattg tccgcggcag agacgcgagt attatcgaga
     3721 ggctcacgcc tcgcgctcgc cttgtccacg agctcgcaga tttgcagcgc cgcgtccatc
     3781 ggcgcgccga gcgttgagtt cgccacgagc gaagccacca gcgtctggcc ctgcttcaat
     3841 tccaggcggt agccatcgac gtcgccgctt ttggccagct tgccgttcac aacaacccgc
     3901 ggctcgaccg tctgcggctt gtcgggcaag tcattggtct cgctctccag cacctcggcg
     3961 agtgtgccga caatgaacgg ccgcaggacc gaggccccat ccgcgttgca gagacgcagc
     4021 cagtacgtgc cggctgccgc ctccgcggcg atctcgacct tcagcttgcc cttgtccttc
     4081 tcggccgtcg ccgtcagccc cggccgatcg gcccagacct gcaccggcca ggtcgagaag
     4141 tcgccgctcg ccgtgacctc gaccgtctgt ccgcgctggc cgccggcagg aaacagcgcc
     4201 gtgaccttgg gcggcgcgga gtaggcaggc gacaataggg cgaaccaaag cagtagggtg
     4261 gaaccagcga gcgcagctcg cgccggccca ccacttcggt gccgcggtgg gccggcgctc
     4321 gcagcgagct tgtcccaccc tgcagctgtc ctcatcccat caactcccgg atcggcgtcg
     4381 ggtcgctgac caggtggacc gggcggccct cgtgcgtgta atacatcttg tcggggtcga
     4441 ggccgagctt cgtgtagatc gtggagacga agttctcggg cgagaggacg cggtcgatgg
     4501 cggcaaaacc cttgctgtcg gtcgcgccga tcgcctggcc gccgggggcc tggccgccgg
     4561 cgaagagcac gctcatcgcg ttggaccagt ggtcgcggcc ggcgtccttg ttgatctgcg
     4621 gagtacggcc gaactcgccc agggcgatca ccagcgtcga atccaggagg ccgcggtccg
     4681 acaagtcctc gatcagcgtg gccaccgagt tgtcccagcc gggcatccgc ttggtgaacg
     4741 agttgaacaa atcggtgtga tggtcccagc cgccgtcata gacggtgatg aagggcacgc
     4801 ccgcctcgac cagacggcgg gcgagcaggc accgctgccc caggttgttg cggccgtact
     4861 tctcgcggag cttcgggtcc tcccggtgga tgtcgaacgc ctgctgggct tccttcgagc
     4921 tgacgaggct atagccttgt tcgtagaagc ggtccacgtc gcggacggga tcggccgcgg
     4981 cttcctcgtt gatccgcacc atgcgatcga cgagcttgcg caggtccttg cggtcggcaa
     5041 agcggccctc cgaaaggtcg cggggaatcg ccacgtcgcg gacgcggaag tccggcttgt
     5101 cggggctctc ttcgacgacg aacggggcgt attgagcgcc caggaaattc ggcccgcccg
     5161 agcgcgacat ctggccgagc gagaagtaac ccggcaggcc cggcggcacg gggcactcgc
     5221 gggcggcagc ggagccgagg ctcggatggt aactgacgaa ggcgccgcaa ccgacgggaa
     5281 tccgcggcgg agcgccggtc atcatgtagt ggttgcccgc gccgtgattg ccctggtcgt
     5341 ggcggatcga gcggatgacc gcgaatttgt cggcgatcga cgccagccgc ttcatgtgct
     5401 gcgagaacga tacgccggcg accttggtcg gaatcgcgtc aaactggccg cggaactcga
     5461 tcggcgcgtc gggcttgggg tcgaacgtct cgtagtgcga cgggccgccg tccatccaga
     5521 tcaggatgca ggacttcgct tgccgcgcgg ggttgaccgc ggcgctggcc gtcgcccgta
     5581 gcgcgcccac caggccgccg ccgagcagtc cgcccagccc cagctggagg cagtcgcggc
     5641 gggtgaagcc ttcacagttg cgatgggtcg tcatcgatgc tctccttcgt ttaacccgga
     5701 gccggaggcg acggcttctc gcggggccag cctgcgcctc cggctacggg ttaaactaat
     5761 ccttgaacat aa
//
LOCUS       DASRJB010000868        12436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_816849, whole genome shotgun sequence.
ACCESSION   DASRJB010000868 DASRJB010000000
VERSION     DASRJB010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816849"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFB80_22775"
     CDS             complement(<1..599)
                     /locus_tag="VFB80_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36679.1"
                     /translation="MGSEVLQIGPRVTCLPVIHGSGDFALVVRRVMLERAFDCVAVPL
                     PPSFQEAVERGIEMLPSPAIVTQAEAPQFATEWSPQSDTEERDDDEPAHSFVPIDPCQ
                     PVIAALRAAISEHIPRAYIDLETARFVPNTAVLPDAYALKKVAIEQFAAAVLPAIARP
                     QETQVRDRIVHMAQRLRQLERKYQSILLVCSVLDWPWIR"
     gene            complement(655..1059)
                     /locus_tag="VFB80_22780"
     CDS             complement(655..1059)
                     /locus_tag="VFB80_22780"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN36680.1"
                     /translation="MNRYLLDTNTCIDYLRGRHQGLVARVRSKRPRELRLCSVVVGEL
                     FYGAHRSADPVANLRLVAEFVDSFISLAFDDHCAEVSGKLRSTLEASGAAVGPYDLQI
                     ASIAVARGLIVVTSNVSEFSRVPGLKLEDWRT"
     gene            complement(1056..1298)
                     /locus_tag="VFB80_22785"
     CDS             complement(1056..1298)
                     /locus_tag="VFB80_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36681.1"
                     /translation="MKTIELIQEAGADKIVRVAIPVDEAARRYRLIVLIEPEVNGDGG
                     EGLNDWPPGFFERTAGKWVGELFRQPQGEYERRASL"
     gene            complement(1365..2312)
                     /locus_tag="VFB80_22790"
     CDS             complement(1365..2312)
                     /locus_tag="VFB80_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN36682.1"
                     /translation="MQRKVEIDGVTLHLSQPHELESQWIGQGEILKQLLACWMVVDER
                     DLPLTPRLVGTPGIGKTALGMAGAKLLGRELYIYQCTADTRPEDLLVTPVLAESGKIA
                     YHASPLVTAVLRGGVCILDEGNRMNEKSWASLAPLFDQRRYVESIVAGITILAAADFR
                     CAVTMNQDESTYEIPDYILSRLQPTLTLGFPSREDELAILQYHLPFAAADMLAMTVEF
                     LQQAHALKLDFSPRDGLNLLRYAMKRLAQDPSHPIAKDRLWREALEKCLGEDALDIEA
                     YAKKRARTLGGESVPLGLGDFFFDPDDPLHPDREEDEEE"
     gene            2531..4390
                     /locus_tag="VFB80_22795"
     CDS             2531..4390
                     /locus_tag="VFB80_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36683.1"
                     /translation="MNDFRGKPQEGTVDFARWLDRGLVAAVLLSSAVALSLNVADPDL
                     WGHVQYGNDLLRHGLPRTTTYSYVAQGYPWINHEIVSEIALAIGADLLGGSGLLIGKC
                     LLGVGLITVILWRTHKLGSSFITTCSVPLLVAVTLANHWSLRPQLASYVCFALLLGLL
                     TYCFHGWEGTWQLSLAGLRRRKGEEPPHQPLDEPPLEYSSQQMKLLWLAAPLFMVWTN
                     AHGGFLAGLCVFLAYLGFRGLEAYSKRGREAEGLLRRFGLMAAAAVGATFLNPYGPFF
                     HFWLAGDLSVPRPEIVEWQAPSILDLQFLPFVALTAAGIAAIVLTKRPRDFTHLAILA
                     LILWQALMHHRHIAFLAIAAGWWLPLHFDSLLERLGVQRRFKTNEELKFGWAPPESSS
                     FTAAFSPQMQQAFALVLVLAICIATGQLTYRLTTLKVDKDNYPVTAFGYIARRGLTGK
                     MVCTFNWAQYALAAFGAREADDRGIQVQIDGRCRTSYSQEMLDMHFDFLMGRIDPSLR
                     YRSASSGPLDPTRVLRVERPDLVLISRLQVPSVEAMESQKDKWVLLYQDSLAQLWGRR
                     SKYDDPASAFYLPMEKREVGESIQRGFVRWPAVPPYQPQNPPPTPLRAVAAEP"
     gene            4419..5288
                     /locus_tag="VFB80_22800"
     CDS             4419..5288
                     /locus_tag="VFB80_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN36684.1"
                     /translation="MPIAESAEVDRNLPLVTPAAEKDPDTIRRASATLAQMEDLAALL
                     AVEDGYEPAERNFVIPRGFKLSVVIPVYNEERTIQTVLARVAALPVPKEIIVVDDAST
                     DGTRDLLEQYEAAHEIHVIFKPQNEGKGAALRTGFRRVSGDVVVVQDADLEYDPRDIL
                     PVVRPIVCGQADVVFGSRYTGEASQDASRLHRFGNRLLTKASNWLTGLELTDMETCYK
                     AFRREVLRSFEIQQNRFGFEPEVTAKIARRGYRIGEVPIRYSPRGYAEGKKIGLGDLL
                     STLWCIVRYGLWD"
     gene            complement(5372..6271)
                     /locus_tag="VFB80_22805"
     CDS             complement(5372..6271)
                     /locus_tag="VFB80_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36685.1"
                     /translation="MLIAFSSYRERMQWPQIHFYEHDGSGAGKIIENIPTVPKRSDNR
                     ASLSVGGRYVAFAAEQENEVSRIRLWDRQEKKLVELPTIDDTPNAQLWPTLAAGGRTI
                     VYAGWNRPGSSQRWDLFLYDVAAKKLGPLSDINTLPHDERMPALAASGRKLAYVTNDP
                     SGAGLMDIALCELATGQAIPLPGLNTAHREVEPSLSADGRWLAFVSDRPGGAGGRDVY
                     LYDGQAQQLVELPGLNSVAHEQTPSISPDGRWLAFVSERIRGEGERDIFLYDIARRQL
                     VPTPGLNAKQEDIDPCVITLPAP"
     gene            complement(6343..7638)
                     /locus_tag="VFB80_22810"
     CDS             complement(6343..7638)
                     /locus_tag="VFB80_22810"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36686.1"
                     /translation="MLNVGSGLTRNCGGWTRRELLKVAGLGAGLTLADALRNDAAASD
                     GKSYAGRERSCIFLWLDGGPSHFETFDPKPDAPDAIRGPYGAIRTKVPGVQFCELMPL
                     TAQRMHKCAIIRSLTHGSGSHSPIPMLTAFENERTSYGAVVAKLAGFERQLPPYVHIG
                     SKLGVGGGSLGAPYNPVEVRDPAGKQIELPQFSLSANISADRFEDRRQLLAAIDQMRA
                     RAGAETAKLDRFQKLAAEILTSPAVREAFDLNKEKDDLRERYGANFFGQACLMARRLV
                     EAGTRFVQIKWYDGPAWDGWDVHGADLGGMERMEHHLCPRLDQGLTALLDDLDQRGLL
                     STTLVVAVGEFGRTPQINKYGARDHWPACFSALLAGGGVPGGVVVGASDKQGGQPARN
                     PVSPPQFAATLYRLLGFNLTTDPRLRPFVKDAAPIGELV"
     gene            complement(7939..8079)
                     /locus_tag="VFB80_22815"
     CDS             complement(7939..8079)
                     /locus_tag="VFB80_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36687.1"
                     /translation="MLTVYECDCGAGFTAQEPLAQRPGWPVADLPMAGEAAERTRLNL
                     SH"
     gene            8379..9221
                     /locus_tag="VFB80_22820"
     CDS             8379..9221
                     /locus_tag="VFB80_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36688.1"
                     /translation="MRSLATAVWAVLAILVFRPARAEDPVKPDEVRQKRLELLLARVD
                     EFQLEVAGQEGGQLKRGAQPILRWSNPVRDFLNDGVIFLFLERSRPRAIVTVWARSPE
                     ATLQSGELWREFVSLSAQPLACSRGGRVLWSPKTGGLVEQPVAGAPAPAGKPAQRLVQ
                     MREIARRFQATSYKMESPFELRLLTQPLYRYEDEAAGILDGALFSFAEGNDPEVVLLL
                     EAMAGAGGKEAAWRYSLARMTSYRVVVRLDGREVFSAVPYWKNPRGPSDPYFEASDGP
                     FTLR"
     gene            9345..11183
                     /locus_tag="VFB80_22825"
     CDS             9345..11183
                     /locus_tag="VFB80_22825"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZN36689.1"
                     /translation="MRRRARTSLAQRKAAQGVVMPFNGMKRASHEIQGRQARRRPRSR
                     RGNIVVFSAFLMIIFMAMLALSVDVGYVFTMKAELRRAVDAAALAGVGELVNGTEAAE
                     AAAKEYLIRNPVGSGLGSVGSEALANATIEFDSNHSDDYEFTFGNWNPETMQLDVTPV
                     MPSTIRVSMQYPNLPFFFARALGHSTFNLQAQSTAMFQPRDIVVVLDFSGSMNDDSTF
                     AAIGGLSQATVESSLLNCWSDLGPPAYGNLPQAPTWATAQGVPANEGTGLPHCTVEYR
                     HTSVVVTSTLNLTTVKLEFSNGNQQSFTPGSVTSGTFAGTGGNANKQVRKVWVKSGTN
                     TSLGANGEFFDFTSANINTTLKKALGLNSVPYPYANGGSWDGYLNYCTSSSNENKNAG
                     YRYKFGGMNLVEYWLANYPAYSQVPDLWKCRAEPMYALKDATGSFMDYIADVDTSDRV
                     ALVVYNAANGNALLESNFTDQLDTIADIVDHRQAGHYHNYTNIGAGLQLGRQTMAASA
                     RPNASKLIVLMTDGLANWHNGGVNTAAAAQMIADEADLCAAAKFKVTTISLGAGADTA
                     TMQSVADTTGGKHYAVPGGATHEAMHAQLSAAFEEIAKARAIKLVE"
     gene            11187..11576
                     /locus_tag="VFB80_22830"
     CDS             11187..11576
                     /locus_tag="VFB80_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36690.1"
                     /translation="MKRLINIAAALLVALAAQPLLATGPHGTWTWTTVVDQEVRVSTL
                     KLKFDSEGKTLEGYFCPSQREQIVPLTRAIYRDGDITVTVVMEHQGQKYEVTFQGSRS
                     GHEIFGTRTIVFEGKTYVAQWSASRWR"
     gene            11791..>12436
                     /locus_tag="VFB80_22835"
     CDS             11791..>12436
                     /locus_tag="VFB80_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZN36691.1"
                     /translation="MRRHGFLIDMDGVIYRGKQLIPGADLFISSLIDRGIPFAFLTNN
                     SQRTRRDVVRKLARMGILVEESHIFTCAMATARFLAAQKPGGTALVIGEGGLLAALHK
                     NGYAIVDDDPDYVVVGEGRTFNMEIVEAAVRMILRGSKLVATNIDPNCPTAGGLRPGC
                     GAIVAMLETATGRSAFSVGKPSPVMMRAARKELGLATGETIMIGDTMETDILGGV"
BASE COUNT         2161 a         4168 c         3985 g         2122 t
ORIGIN      
        1 tcgcgaatcc agggccagtc gagaaccgag cagaccagca ggatcgactg gtatttcctt
       61 tcgagttgcc gcagccgctg ggccatgtgc acgatgcgat cccgcacctg tgtttcctgc
      121 ggccgcgcga tcgccggcag caccgccgcc gcgaattgct cgatcgccac tttcttaagg
      181 gcataggcat cgggcagcac ggccgtattg ggaacaaacc gggcggtctc caggtcaatg
      241 taagctcgtg gaatgtgctc gctgatcgcg gcccgcagcg ccgcaatcac cggctggcag
      301 ggatcgatcg gcacaaagct gtgcgccggc tcgtcgtcgt cgcgctcttc cgtgtcgctt
      361 tgcggcgacc actccgtcgc gaactgcggc gcttcggcct gcgtcacgat tgccgggctc
      421 ggcagcatct cgatcccgcg ctcgaccgcc tcctgaaacg acggcggcag cggaaccgcc
      481 acgcaatcaa atgcccgctc gagcatcacc cgccgcacca ccagcgcaaa atccccgctg
      541 ccgtgaatca ccggcaggca agtgacgcga ggaccgattt ggagaacctc ggaacccatg
      601 aaagaactga aaattggtca aacgtcgtgc gctccaccgg actcgagcgc gctctcaagt
      661 gcgccaatcc tctaacttaa gtccaggcac gcgcgagaat tcactgacat tgctcgtcac
      721 gacgatcagg ccgcgagcta cggcgatcga tgcaatttgc agatcgtacg gcccgactgc
      781 ggccccactt gcttcgagcg ttgaacgaag cttgccggat acttctgcgc agtgatcgtc
      841 aaacgcgaga ctaatgaagc tgtccacgaa ctcagcgacc agcctgagat tggcgaccgg
      901 gtcggcacta cgatgggcgc cgtagaacaa ctcgcccacg acgaccgagc aaagtcggag
      961 ttctcggggc cgcttggacc gaacgcgggc cacgaggccc tgatgcctgc ctcgcaagta
     1021 gtcgatgcaa gtattggtat cgagcaggta ccgattcaca gcgacgctcg ccgttcgtat
     1081 tcaccttgtg gctggcggaa aagctcgccg acccatttgc ccgcggtgcg ctcaaagaag
     1141 cccggcggcc agtcgttgag tccttcgccc ccgtctccat tgacttccgg ctcaatcagc
     1201 acgatcaggc gatatcgccg cgctgcttca tctacgggga tcgccacgcg gacgatcttg
     1261 tcggctcctg cctcttgaat cagctcgatg gttttcatag gactgctcgg atgcgagatg
     1321 gcgccactcc attataccat cgacgaatgc ggaaccgccc cgagctactc ctcctcatcc
     1381 tcctcgcgat ccgggtgcag cgggtcgtcg gggtcgaaga agaagtcgcc gaggcccaag
     1441 gggacggatt caccgccgag ggtgcgggca cgtttcttgg cataggcctc gatgtcgagg
     1501 gcgtcttcgc ccaggcattt ctcgagcgcc tcgcgccaca agcggtcctt cgcgatcggg
     1561 tgcgatgggt cctgggccag gcgcttcatc gcgtaccgca gcaggttcag gccgtcgcgg
     1621 ggcgaaaagt cgagcttgag cgcgtgggcc tgctgcaaga actcgaccgt catcgccagc
     1681 atgtcggccg ccgcgaacgg caggtgatac tgcaagatcg ccagctcgtc ctcgcggctg
     1741 gggaagccca gcgtcagcgt cggctgcaag cggctgagga tgtagtcggg aatctcgtag
     1801 gtcgactcgt cctggttcat cgtcacggcg cagcgaaagt cggccgcagc caagatcgtg
     1861 atgccggcga cgatcgactc gacgtaccgc cgctggtcga agagcggcgc caggctggcc
     1921 cacgactttt cgttcatccg gttgccttcg tcgagaatgc agacgccgcc gcgaagcacg
     1981 gccgtaacca gcggcgaggc gtgataggcg atcttgccgc tctcggccag gaccggcgtc
     2041 accagcaggt cctcgggccg cgtgtcggcg gtgcactgat agatgtacag ctcgcggccc
     2101 agcagcttcg ccccggccat ccccagggcc gtcttgccga tgcccggcgt tcccacgagc
     2161 cgcggcgtca gcggcaggtc gcgttcatcc acgaccatcc agcaggccag gagctgcttg
     2221 agaatctcgc cctggccgat ccactggctc tccaattcgt gcggctggct gaggtgcagc
     2281 gtcacgccgt cgatttcgac ttttcgttgc ataggtagcg ggcacgctcc tcagaggacg
     2341 gcacacggag tgtgcctact actctagaga gcccttccag ccgctccaag cggtagcagc
     2401 ggtacgcgcg gcgcgagggg cgaagctgga atccctgccc tgcgcctcaa gttctgcgcc
     2461 cgccgcagcc gcataaccta ggctttcacg tcccggtcta ttgcgcacta ctagtcagcg
     2521 gggatgccgg atgaatgact tccgtggaaa accccaggaa gggaccgtcg attttgcgcg
     2581 gtggctcgac cggggcctgg tggccgcggt gttgctctcc agcgcggtcg ccttgtcgct
     2641 gaacgtggcc gacccggacc tgtgggggca cgtgcaatac ggcaacgacc tattgaggca
     2701 cggcctgccg cggacgacga cctattcgta cgtggcccag ggctatccct ggatcaacca
     2761 cgaaatcgtc agcgaaatcg cgctggcgat tggggccgat ctgctgggcg gatcgggcct
     2821 gctgatcgga aaatgcctgc tcggcgtcgg actgattacg gtaatcctct ggcggacgca
     2881 caagctggga tcgagcttca tcacgacctg cagcgtgccg ctgctggtcg ccgtcacgct
     2941 ggccaaccac tggagcctcc gcccgcagct tgcctcgtac gtctgttttg ccctgctgct
     3001 gggactgctg acgtattgct tccacggctg ggagggcacc tggcagctgt cgctcgccgg
     3061 gctccgccgc cgcaaaggcg aagagccgcc ccatcagcca ctcgacgagc cgccgctcga
     3121 gtattcctcg cagcagatga aactgctctg gctcgcagcg ccgctattca tggtttggac
     3181 caacgcgcac ggcggcttcc tggccggatt gtgcgtgttc ctggcctact tgggcttccg
     3241 cggcctggag gcctactcga agcgcggccg cgaggcggaa ggcctgctgc ggcgcttcgg
     3301 cctgatggcc gccgcggcag tcggcgcgac gttcctcaac ccgtacgggc cgttttttca
     3361 cttctggctc gcgggggacc tcagcgtgcc gcggccggag atcgtcgaat ggcaggcgcc
     3421 ttcgatcctg gacttgcagt tcctgccatt cgtcgcgctg acggccgccg gcatcgctgc
     3481 gatcgtcctg accaagaggc cgcgcgactt cacgcacctg gccatcctgg ccctgatcct
     3541 ctggcaagcc ctgatgcacc accgccacat cgcctttttg gcgatcgccg ccggctggtg
     3601 gctgccgctg cacttcgact cgctgctgga gcggctgggc gtccagcgcc gcttcaagac
     3661 caacgaggag ctgaagttcg gctgggcccc gccggaaagc tcatcgttca ccgccgcgtt
     3721 ttcgccgcag atgcagcagg cgttcgcgct ggtgctggtg ctggcgatct gcatcgccac
     3781 cgggcagctg acctaccgcc tgaccacgct gaaagtggac aaggacaact accccgtcac
     3841 ggcgttcggc tacatcgccc gccgcggcct gacgggcaag atggtgtgca cgttcaactg
     3901 ggcccagtac gccctggcgg cgttcggggc ccgcgaagcc gacgatcgcg gcatccaggt
     3961 gcaaatcgac ggtcgctgcc gcacgagcta ctcgcaagag atgctcgaca tgcacttcga
     4021 tttcctgatg ggccgcatcg atcccagcct ccgctatcgc agcgcctcgt ccgggccgct
     4081 cgatcccacc cgcgtcctcc gcgtcgagcg cccggacctc gtgctaatct cccgcctgca
     4141 ggttcccagc gtggaggcga tggagtcgca gaaagacaag tgggtgctgc tctaccagga
     4201 ttcgctggcg cagctctggg gccgccgctc gaaatacgac gacccggcca gcgcgttcta
     4261 cctgccgatg gaaaagcgcg aggtcggcga gtcgatccag cgcggtttcg tccgctggcc
     4321 ggccgtgccc ccgtatcagc cgcagaatcc gccccccacg cccctccgcg ccgtcgccgc
     4381 tgaaccgtag ttccacacca tgctgccgag agccgcccat gccgatcgcc gagtccgccg
     4441 aagttgaccg caatctaccg ctcgtaacgc cggcagccga aaaagatccg gacacgatcc
     4501 ggcgggccag cgcgacgctc gcccagatgg aagacctcgc cgccctcctg gccgtcgagg
     4561 acggttatga gccggcggag cggaacttcg tcatcccccg cggcttcaag ctgtcggtgg
     4621 tgattcccgt ctacaacgaa gagcggacga tccagaccgt gctggcccgc gtcgctgccc
     4681 tgccggtgcc caaggagatc atcgtcgtcg atgatgccag caccgacggc actcgcgatc
     4741 tgttagagca gtacgaagcg gcgcacgaga tccacgtgat cttcaagccg cagaacgaag
     4801 gcaagggggc tgccctgcgg accggcttcc gccgcgtcag cggcgacgtc gtcgtcgtgc
     4861 aggacgccga cctcgaatac gacccccgcg acatcctgcc cgtcgtgcgg ccgatcgtct
     4921 gcggccaggc cgacgtcgtc ttcggctcgc gatataccgg cgaagcatcg caggacgcct
     4981 cgcggctgca ccgcttcggg aaccgcctgc tcaccaaggc gtcgaactgg ctgacgggcc
     5041 tcgaattgac cgacatggag acctgctaca aggccttccg ccgcgaggtg ctccggtcgt
     5101 tcgagatcca gcagaaccgc tttggctttg agccggaagt caccgccaag atcgcccgcc
     5161 gcggctaccg catcggcgag gtgcccatcc gctactcgcc ccgcggctac gccgaaggca
     5221 aaaagatcgg cctcggcgac ttgctcagca cgctgtggtg catcgtgcgg tacggcctgt
     5281 gggattgagg gcgattcctc gtttaacccg gagccggagg cgacggtgtg accaggggac
     5341 cgccgtcgcc tccggctccg ggttaaacga ctcaaggcgc cggcagcgtg attacgcagg
     5401 gatcgatgtc ttcctgcttc gcgttcaagc ccggcgtggg cacgagctgg cggcgggcga
     5461 tgtcgtacag aaagatgtcg cgctcgcctt cgccgcggat ccgctcggag acgaaggcta
     5521 gccagcggcc gtccgggctg atcgacggcg tctgctcgtg ggcgacggag ttcagaccgg
     5581 gcagctcgac cagctgctgc gcctggccgt cgtagagata gacgtcgcgg ccgcccgcgc
     5641 ctccgggccg gtccgagacg aacgccagcc agcggccgtc ggccgagagt gacggctcga
     5701 cctcgcggtg ggcggtgttc aggcccggca gcggaatcgc ctggccggtg gccagttcgc
     5761 agagggcgat gtccatcagc cccgcgcccg acggatcatt ggtgacgtac gccaacttgc
     5821 ggccgctggc ggccagcgcg ggcatccgct cgtcgtgcgg cagcgtgttg atgtcggaca
     5881 gcggaccaag cttctttgcc gcgacgtcgt acaagaacaa gtcccaccgc tgactcgagc
     5941 cggggcggtt ccagccggca tagacaatcg tgcggccgcc agccgccagc gtcggccaga
     6001 gctgcgcatt gggcgtgtcg tcgatcgtcg gcagctcgac cagctttttc tcttgccggt
     6061 cccacagccg gatgcggctg acctcgttct cctgctccgc cgcaaaggcg acatagcggc
     6121 cgcccacgct caggctggcc cgattgtcac tccgcttggg aaccgtgggg atgttctcga
     6181 tgatcttgcc cgcgccgctg ccgtcgtgct cgtagaagtg aatctgcggc cactgcatcc
     6241 gctcgcgata cgacgagaac gcgatgagca cccgcggctc ttgactaaac gcaagcggcg
     6301 cgaagagaca aaaagcaagc gccgtgaaaa cgaatgcccg catcacacca attcgccgat
     6361 cggggcggcg tccttgacga acggccgcag gcgggggtcg gtcgtgaggt tgaagcccag
     6421 cagtcgatag agcgtggcgg cgaactgcgg cggcgagacg ggattgcggg ccggctgccc
     6481 gccttgcttg tcgcttgcac cgacgaccac gccgcccggc acgccgccgc cggcgagcag
     6541 cgcggagaag caggccggcc agtgatcgcg agcgccgtat ttgttgatct gcggcgtgcg
     6601 gccgaactcg ccgacggcga cgacgagcgt cgtggacaag aggccccgct gatcgaggtc
     6661 gtcgagcagc gcggtcagcc cctggtcgag ccgcgggcag aggtggtgct ccatccgctc
     6721 catgccgccc aggtcggcgc cgtgcacgtc ccagccgtcc caggcggggc cgtcgtacca
     6781 cttgatctgg acaaaacgcg tgccggcctc gaccaggcgg cgggccatga ggcaggcctg
     6841 cccaaaaaag ttcgccccat accgctcgcg gaggtcgtct ttctctttat tcaagtcaaa
     6901 cgcctcgcgg acggcgggcg aggtgagaat ctcggccgcg agcttctgga agcggtcgag
     6961 cttcgcggtt tccgcaccgg cccgtgcccg catctggtcg atcgcggcca gcagttgccg
     7021 gcgatcctca aagcggtctg cggaaatgtt cgccgacagc gagaactgcg gcagctcgat
     7081 ctgcttgccc gccgggtcgc ggacctcgac cgggttgtac ggcgcgccga ggctgccgcc
     7141 gcccacgccg agcttggagc cgatgtgcac atagggcggc agctgccgct cgaacccggc
     7201 cagcttcgcc acgactgcgc cgtagctggt ccgctcgttc tcgaacgcgg tcagcatcgg
     7261 gattggcgag tgggagccgc tgccgtgcgt gagcgagcgg atgatcgcgc acttgtgcat
     7321 ccgctgggcg gtcagcggca tcagctcgca gaactgcaca ccagggacct tggtgcggat
     7381 cgcgccgtaa ggtccgcgga tcgcatccgg cgcatcgggc ttgggatcga aggtctcaaa
     7441 atggcttggc ccgccgtcga gccagaggaa gatgcacgac cgctcgcggc ccgcgtagct
     7501 cttgccatcg ctagctgccg cgtcgttgcg cagagcgtcg gccagcgtga gtccggcacc
     7561 gagccccgcc accttgagca gctcgcgccg cgtccagccg ccgcaattcc gcgttaggcc
     7621 gctccccacg ttgagcatcg cgccgcctcc catcaagact cgctgattgt aggcagttca
     7681 agcatcgccg cacaacagtt tgatgctctc gcgctccaag cttggcactg cggcaaaatg
     7741 acggcacgaa ttttgctcga tttcgtacca atccagcctt cattggggaa gcgcgagaat
     7801 atggcgacac gaaagccttc gtcgtcgatc cgtttggcca ttctggcgtg catttctcgg
     7861 tgcagccaaa gcctcgctcc cctgacaagc ctgctcgaag tcaccgcgcg actgcgacgt
     7921 cttggctggg acgcagcctc agtgcgacag gttgaggcgg gtgcgctccg ccgcctcgcc
     7981 agccatcggc aggtcggcga ctggccagcc gggccgctgc gcgagcggct cctgagccgt
     8041 aaatcccgcc ccacagtcgc actcgtagac cgtcagcatc gcaatcggct gcttccggtc
     8101 cgctaacgaa tactgcggcc tggctgagaa cagacgcgac gctgcgtttc cgcacttcgg
     8161 gcaccgcgga gcaggcgaat ctttcggctg catggtattt cccccagagc tttactcaac
     8221 gtggttgatg ggtgagtacg cgtcaatacg agaaaattag cagtggtgcg gtaccgtaca
     8281 tggctcgctg cgcgcctcca accgattgcc tacctcgacc ccctatagac ataatgtcta
     8341 attcacacgg agtgttactt ggttcctggg agggctacat gagatccctg gctactgcgg
     8401 tgtgggccgt actggcgatc ttggtgttcc gcccggctcg cgccgaggac ccggtgaagc
     8461 cggacgaagt gcggcaaaag cggttggagc tgctgctggc gcgggtggat gagttccagt
     8521 tggaagtggc gggccaggag ggcgggcaac tgaagcgcgg ggcgcagccg attttgcggt
     8581 ggagcaatcc ggtccgggac tttctcaacg acggcgtcat tttcctgttc ctggagcgca
     8641 gccgcccgcg ggcgatcgtc accgtatggg cccgcagtcc ggaggcgacg ctgcagagcg
     8701 gcgaattgtg gcgggagttc gtttcgctca gtgcgcagcc gctcgcttgc agtcgcggcg
     8761 gccgcgtgct ctggtcgccg aagaccggcg ggctggttga gcagccggtg gcaggcgctc
     8821 ccgcgcccgc cggcaaaccc gcccaacgtc tggtgcagat gcgcgagatc gctcgccgct
     8881 ttcaggccac aagctacaag atggagtctc ctttcgagct gcggctgctg acccagccgc
     8941 tctatcgcta cgaggacgag gctgccggca ttctcgacgg cgcgctcttc agctttgcgg
     9001 aaggcaacga ccccgaagtc gtgctgctcc tggaggcgat ggccggagcg ggcgggaagg
     9061 aggctgcgtg gcgctattcg ctggcccgca tgacgtccta ccgcgtggtc gtgcggctgg
     9121 acggccgcga ggtctttagc gccgttccct attggaagaa cccgcgcggc ccaagcgacc
     9181 cctatttcga agcttccgac ggaccgttca cgctgcgcta gccgcgccac cggcaaagaa
     9241 ccgataaagc gacgcgcttg cgccccgcgc atcgcttttc aggcacgacc ggttcttttg
     9301 acctagagtt aaattgccac cagttcgttc gcaggcatga cgccgtgcgg cgccgggctc
     9361 gaacgtcgct ggcccagcgg aaagccgcac agggggtcgt catgccgttc aacgggatga
     9421 agcgcgccag tcacgaaatc caaggccgac aggccagacg ccggccgcga tcgcggcggg
     9481 ggaacatcgt cgtcttctcc gcgttcctga tgatcatctt catggccatg ctggccctgt
     9541 cggtggacgt gggatacgtc ttcacgatga aggccgagct gcgccgcgcg gtcgatgccg
     9601 cggccctggc cggcgtcggc gaattggtca acggcaccga agcggccgaa gcggccgcca
     9661 aggaatatct catccgcaac ccggtcggct cggggctggg ctcggtgggc agcgaggccc
     9721 tggccaacgc cacgatcgag ttcgattcca accactccga cgactacgag ttcacgttcg
     9781 gcaactggaa cccggagacg atgcagttgg acgtgacccc ggtcatgccc tccacgatcc
     9841 gggtctcgat gcagtatccg aatctgccgt tcttctttgc ccgcgcgctg gggcacagta
     9901 cgttcaattt gcaggcccaa tcgacggcca tgttccagcc ccgcgacatc gtcgtggtgc
     9961 tcgacttctc cggctcgatg aacgacgaca gcacgttcgc ggccatcggc ggcttgagcc
    10021 aggccacggt cgaatcgagc ctgctcaact gctggagcga tctgggtccg cccgcctatg
    10081 gcaacttgcc acaggcgccg acctgggcca cggcccaggg cgtgccggcc aacgagggga
    10141 cgggattgcc gcactgcacc gtggagtatc gccacacgtc ggtcgttgtg acctcgacgc
    10201 tcaacctgac gaccgtcaag ctcgaattct ccaacggcaa ccagcagtcg ttcacgcccg
    10261 gcagcgtcac gtcgggcacg ttcgccggga ccggcggcaa cgccaacaag caggtccgca
    10321 aagtctgggt caagtcgggc acgaacacct cgctgggcgc caatggcgag ttcttcgact
    10381 tcaccagcgc caacatcaat acgacgctga agaaggccct gggtctgaac tccgtgccct
    10441 atccctatgc caacggcggc agctgggacg gctacctcaa ctactgcact tcgtcgtcga
    10501 acgagaacaa gaacgccggc taccgctaca agttcggcgg catgaacctg gtcgaatact
    10561 ggctggcgaa ctatcccgcc tacagccagg ttcccgatct gtggaaatgc cgggcggagc
    10621 cgatgtacgc cctcaaggac gcgacgggct cgttcatgga ctacatcgcc gacgtcgata
    10681 cgagcgaccg cgtggccctg gtggtctaca acgccgctaa cggcaacgcc ttgctggaga
    10741 gcaacttcac cgaccagctc gatacgatcg ccgacatcgt cgatcaccgg caggccggcc
    10801 attatcacaa ctacacgaac atcggggccg gcttgcagct cggccgccag acgatggcgg
    10861 cgagcgcccg ccccaacgcc agcaagctga tcgtgctgat gaccgacggc ctggccaact
    10921 ggcacaacgg cggagtgaat accgcggccg ccgcccagat gatcgccgac gaggcggacc
    10981 tctgcgccgc cgcgaaattc aaagtcacga ccatcagcct cggcgccggc gccgacacgg
    11041 ccaccatgca gagcgtcgcc gatacgaccg gcggcaagca ctacgccgtg cctggcggcg
    11101 cgacgcacga agcaatgcac gcccagctct ccgccgcctt cgaggaaatc gccaaggccc
    11161 gggccatcaa actggtggaa taacccatga aacgtctcat caacatcgca gccgcgttgc
    11221 tggtcgccct cgcggctcag ccgctcttgg ccaccggtcc ccacggcacg tggacctgga
    11281 cgaccgtcgt cgatcaagag gtccgcgtca gtacgctcaa gttgaagttc gactcggaag
    11341 gcaaaacgct ggagggctac ttctgcccca gccagcgcga gcaaatcgtg cccctcacgc
    11401 gggccattta tcgcgacggc gatatcacgg tcacggtggt catggaacat cagggtcaga
    11461 agtatgaggt gacgttccag ggcagccgca gcgggcacga aatctttggc acgcggacca
    11521 ttgtgttcga ggggaagacc tacgtggccc agtggtcggc ctcccgctgg cggtgaggcg
    11581 cgagggacgt aagcgaaacc caccgcggcc aggctgccgg gtagcgcggc agcagtgagt
    11641 gattgctcgg cagctcgcgg ctgccggaaa tcgccgcaag tccttcgcgg ctggcggcgc
    11701 cagcttgcct gccttgccag attcggctgc ggcacgcggg ttgcaagtgc tccgggcccg
    11761 aggcggttcg ttctcctccc cggagcagca atgcggaggc atggtttcct gatcgacatg
    11821 gatggcgtga tctatcgcgg caaacaactc attcccggcg ccgacctctt catctcctct
    11881 ctcattgacc gcggcattcc gttcgccttc ctgaccaaca acagccagcg gacgcggcgc
    11941 gacgtcgtcc gcaagctggc ccggatgggc atcctcgtcg aggagagcca catcttcacc
    12001 tgcgcgatgg ccacggcccg cttcctcgcc gcccagaagc ccgggggaac ggcgctggtc
    12061 atcggcgaag gaggcctctt ggcggccctg cacaaaaacg gctacgccat cgtcgatgac
    12121 gacccggact acgtcgtcgt cggcgagggg cggacgttca acatggagat cgtcgaggcg
    12181 gcggtgcgga tgatcctccg cggcagcaag ctcgtcgcca cgaacatcga ccccaattgc
    12241 cccacggccg gcgggctgcg tcccgggtgc ggagcgatcg tggcgatgct cgaaacggcg
    12301 acgggccgct cggcgttcag cgtcggcaag cccagcccgg tgatgatgcg ggcggcccgc
    12361 aaggagctgg gcctggccac cggggaaacc atcatgatcg gagacaccat ggagaccgac
    12421 atcctggggg gcgtca
//
LOCUS       DASRJB010000869         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2514465, whole genome shotgun sequence.
ACCESSION   DASRJB010000869 DASRJB010000000
VERSION     DASRJB010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514465"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1459
                     /locus_tag="VFB80_22840"
     CDS             71..1459
                     /locus_tag="VFB80_22840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZN36692.1"
                     /translation="MDFVENFGHKRQLIEQILNTPPAGRPGDFTHVVDRILQRRDDLL
                     ALCQQHATPLFAYDPRSFREALDEFTAAFGRHVPRHRPFFAVKSNHYPELAADAMAGR
                     FGLDVSSSRELRLALAAGAGQILFSGPGKSAADLELASANRERVIVNLDSFRELALLG
                     EVAAHNNSGRGLRVSAGGLRGGPMRAGVRIHTAAHGSWSKFGIPLADLPRFWRAAAGL
                     PGIDLQGIQSHLSWTHDASPYCRVIEEVARTLASEFTPAERAQVRFYDFGGGFRPFQL
                     EGRYPTELPLGELIRVADDVAGEASRFAAPYYFQESVPLELYAREIGVALIRHLSPLL
                     DCDFFSEPGRVVAAHAMHLVMRVADQKRDDLVIVDGGIHMIGWERYLHVYCPVVNLTR
                     PARREIPVTICGSLCDPEDLLGRHCFAESIQEGDVLVMPFQGAYTYSTAHNFIREIPP
                     VYSLSSAASSLR"
     gene            complement(1423..2010)
                     /locus_tag="VFB80_22845"
     CDS             complement(1423..2010)
                     /locus_tag="VFB80_22845"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZN36693.1"
                     /translation="MPEYEWITHKIFVVREFFTPEECRAAIDRAEATGFGQAPINTAF
                     GPQIRTDVRNNSRVMIDDPQLAAALWERCRDYIPLRLGDWEAVGVNERFRYYRYDVGQ
                     QFDWHYDGAFQRVSGERSRLTFMIYLNTGFDGGTTSFEDAQIVPQEGLALFFWHAILH
                     KGQPVERGRKYVLRTDVMYLHNRVQRSDEAADDKE"
     gene            complement(2080..>3796)
                     /locus_tag="VFB80_22850"
     CDS             complement(2080..>3796)
                     /locus_tag="VFB80_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36694.1"
                     /translation="AALPPPKVPPLPATKVSEAALAPPPPAEANGALPQAAAARPQQT
                     RYWMLMAGSVALGIVLAFGAVSGAMFFFGKNAPLAAAGNGGNSDPRSAPVTTPVSSPA
                     PIPPAGKTPDVTPTPIETPAPTVTPTPPTPEVTTPTPPTETDPAGIVKPTPAAAAPSV
                     SNDPLAKLERLIGGAGEDPQASSASAPPPVVPPADSAPAKAALPRPPPRDIDVARRLA
                     DPLAGIETAGTPLADFLDRFAELSTIPITLDPDWLAFALATPETPIAYRAASTTVGEA
                     LTKALAPLRLAPVVSGDQIVVGLSQPQPLVTIQVKAKELAASEEDLAELAELLKALIE
                     PSSWTGENAGTISVDAAASALNVTHLKIVQAQVLLVCEKLRTARQKKHELLQLDQALF
                     QLDSRSARAAGKLAAPVSLNFSQPTRLTAVLDRLGESAGVRILVDWQGIAAAGWNPDA
                     DATLVADKQPLGEALHALLEPMELAWRAIDGRTLQVVTAAKLAERCEIEFYPAADLLA
                     GDPTGEALQTRLRDSLGAAAFQSGGGSGELRLDRLSKCLIVSLPQLKQRELEALLARW
                     RAETR"
BASE COUNT          574 a         1251 c         1309 g          662 t
ORIGIN      
        1 gtacggcggg cggggcgacc tcaatgtcgc cggccggcga ctggtgcgcg aattccgtta
       61 cacttgatgc atggatttcg tcgagaattt cgggcacaag cggcaactca tcgagcaaat
      121 cctgaatacg ccgcccgcgg ggcggccggg cgactttacg cacgtcgtgg accggattct
      181 tcagcggagg gacgatctat tagcgctctg ccagcagcat gcgacgccgc tgttcgccta
      241 cgatccgcgc tcgttccgtg aggcgctgga cgaattcacc gccgcctttg gccggcacgt
      301 gccgcggcac cggccgtttt ttgcggtcaa gtcgaaccat tatccggagc tggcagcgga
      361 cgccatggcc ggtcgattcg ggctggacgt ctccagcagc cgcgagctgc ggctggcgtt
      421 ggcggccggc gcgggacaaa tcctgttcag cggaccgggc aaatcggccg cggatttgga
      481 gctggccagc gcaaatcgcg agcgggtgat tgtgaacctc gacagctttc gcgagttggc
      541 tctattgggc gaagtcgccg cgcacaataa ttctggccgc gggcttcgcg tttctgccgg
      601 cgggcttcgc ggcgggccga tgcgcgcggg agtccggatt cacaccgccg cgcacggaag
      661 ctggtcgaaa ttcggcattc ccctggccga cctgccgcgg ttctggcggg ctgcggccgg
      721 attgcctggg atcgatttac aaggcattca atcccacttg agctggacgc acgacgccag
      781 cccgtactgc cgtgtgattg aggaagtcgc ccggacgctc gccagcgagt tcacgccggc
      841 cgagcgggcg caggtccggt tttacgattt cggcggcggc ttccggccgt ttcaactcga
      901 gggccgctac ccaaccgagt tgccgctcgg cgagctgatt cgcgtcgccg acgacgtggc
      961 gggcgaagcg agccgcttcg ccgcgccgta ctacttccag gaaagtgtgc cgctcgagtt
     1021 gtatgcccgc gagatcggcg tggcgctcat ccggcacttg tcgccgctac tggactgcga
     1081 ttttttttcc gaacccggcc gcgtcgtcgc cgcccacgca atgcacctgg tgatgcgggt
     1141 cgccgaccag aaacgcgacg acctggtcat cgtcgatggc ggcattcaca tgatcgggtg
     1201 ggagcggtat ctgcacgtct attgcccggt cgtcaatctc acgcggccgg cccgccgcga
     1261 gattcccgtc acgatctgcg gctcgctgtg cgaccccgag gacctcctcg gccgccactg
     1321 cttcgccgaa tcgatccagg agggggacgt gctggtgatg ccgtttcaag gcgcgtacac
     1381 ctattcgacg gcccacaact tcattcgcga aattccgccg gtctattcct tgtcgtcggc
     1441 ggcctcgtcg ctgcgttgaa cgcggttgtg gaggtacatc acgtcggttc gcaagacata
     1501 cttgcggccg cgctcgaccg gctgtccctt gtggaggatg gcgtgccaga agaagagcgc
     1561 caggccctcc tgggggacga tctgggcgtc ctcgaacgac gtcgttccgc cgtcgaagcc
     1621 ggtattgagg tagatcatga acgtcaagcg gctccgctcg ccgctgacgc gctgaaacgc
     1681 gccgtcgtag tgccagtcga actgctggcc gacgtcgtag cggtagtagc gaaagcgctc
     1741 gttcacgccc accgcctccc agtcgcccag ccgcagggga atgtagtcgc ggcaccgctc
     1801 ccagagggcg gccgccagct gcggatcgtc gatcatgacc cgcgaattat tgcggacatc
     1861 ggtgcggatc tgtgggccga aggccgtatt gatcggcgcc tggccgaagc cggttgcctc
     1921 ggcgcggtcg atcgccgcgc ggcattcctc gggcgtgaaa aactctcgca cgacaaagat
     1981 cttgtgcgtg atccattcgt attcgggcat gggcgctcct ttagcggcgc aggcagtggg
     2041 gacacgccct gattgcccgg ggttcaccgc gagtgggcgc taccgggtct cggcccgcca
     2101 gcgggccaat agtgcttcca gctcgcgctg cttgagctgc ggcaggctga cgatcaggca
     2161 cttgctgagc cggtcgagcc gcagttcgcc gctgccgccg ccgctttgaa acgcggctgc
     2221 accgagcgag tcccgcagcc gggtctgcag cgcctcgccc gtggggtcgc cggccagcag
     2281 gtccgccgcc ggatagaact cgatctcgca tcgctcggcc agcttcgccg ccgtcacgac
     2341 ttgcagcgtg cggccgtcga tcgcccgcca ggccagctcc atcggctcca gcagcgcatg
     2401 gagggcctcg cccagcggct gcttgtcggc caccagcgtc gcatcggcgt cgggattcca
     2461 gccggcggcg gcgattccct gccaatcgac gagaatccgc acgcccgccg actcacccag
     2521 ccggtcgaga accgccgtca gccgcgtggg ctggctgaaa ttcagcgaga cgggcgccgc
     2581 gagctttccc gccgcccggg ccgagcgcga atcgagctga aacagcgcct gatcgagctg
     2641 cagcagctca tgcttcttct gccgggcggt gcggagcttc tcgcagacca ggagcacctg
     2701 ggcctgtacg atcttcagat gcgtgacgtt tagcgcagag gccgcggcat cgacgctgat
     2761 cgtcccggcg ttttcccccg tccacgaact gggctcgatc agcgccttga gcagctccgc
     2821 caattccgcc agatcctcct cgctggccgc gagctctttc gccttgactt gaatcgtcac
     2881 caagggctgc ggctgcgaga gccccacgac gatctggtcg cccgagacga cgggcgccag
     2941 ccgcaacggc gcgagcgcct tggtcagcgc ctcgccgacc gtcgtgctgg ccgcacggta
     3001 agcgatcggc gtttcgggag tggccagggc gaaggccagc cagtcgggat cgagcgtgat
     3061 cgggatcgtc gagagctccg cgaaccggtc gaggaagtcg gccagcggcg tgccggccgt
     3121 ttcgattccc gcaagcggat cggccaggcg gcgagccaca tcgatgtcgc gcggcggagg
     3181 gcgcggcagc gcagccttgg cgggcgcgga atcggccggc ggaaccacgg ggggtggcgc
     3241 ggaagccgag cttgcttgcg ggtcttcacc ggcgccgcca atcaggcgtt cgagcttggc
     3301 cagcggatcg ttggagacgc tcggcgctgc agctgccgga gtcggcttga cgatgcccgc
     3361 cgggtcggtt tcggtcggcg gtgttggcgt tgtcacctcg ggcgttggcg gcgtaggcgt
     3421 gaccgtaggg gcgggagtct cgatcggtgt cggcgtcaca tcgggcgtct tgccggccgg
     3481 cgggatggga gccggcgatg aaaccggcgt cgtaacgggc gctgagcgtg gatcagaatt
     3541 gccgccgttt cccgcggcag ccagcggcgc gttcttaccg aaaaaaaaca tcgcccccga
     3601 aaccgctccg aacgccagga cgattcccag cgcgaccgag cccgccatga gcatccagta
     3661 ccgggtctgc tgcggccgcg cggcggcggc ctgcgggagc gctccgttcg cctccgcggg
     3721 aggcggtggg gccagcgccg cttcgctgac tttggtcgcg ggcagaggcg gcactttggg
     3781 cggcggaagg gccgcg
//
LOCUS       DASRJB010000870         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3923016, whole genome shotgun sequence.
ACCESSION   DASRJB010000870 DASRJB010000000
VERSION     DASRJB010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923016"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1699
                     /locus_tag="VFB80_22855"
     CDS             128..1699
                     /locus_tag="VFB80_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZN36695.1"
                     /translation="MLHYDPGTPRCLVLAPLDEFNAALLARVHPPDWQNPTPQPLYDL
                     VAIGGGTAGLVAAAGTAGLGGKVALVERGLLGGDCLNFGCVPSKALIRAARAAYDVGR
                     AAEFGIHVASGGRQPPEAAAVDFAAVMSRLRRLRAEISENDSAQRFQQLGVDVFLGDA
                     RFTSRQTIEVAGQTLRFKRCVIATGGRAAIPAVPGLEEAGCLTNETVFSLTELPRRLI
                     VIGGGPIGCELAQAFRRFGSEVTLVQRGQRLLPKDEPEAAEILARQFEREGIKLLFGA
                     TSVRAETAGGIKRLLVNSGGRQQGLDADAVLVAAGRTPNIEGLNLAAAGVEHNNRGVI
                     VDDFLQTANPRIMAAGDVAGGYQFTHAADAMARACVQNAFFSLAGFGRKRLSGLVVPW
                     TTYTDPEIAHVGLTPASAADQHLEIDSYREELRHVDRAILDGDDEGFAVIHCRRGTPQ
                     VVGCTIVARHAGEMIGEVSLLMTARIPLSKLAGTIHCYPTQAEILKRLGDQYNKRRLT
                     PRVADVLKAIIGWRS"
     gene            1810..2463
                     /locus_tag="VFB80_22860"
     CDS             1810..2463
                     /locus_tag="VFB80_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36696.1"
                     /translation="MTRQIAFVAAALLVFAAASAAQDKPAVEEGFTPLFDGKTLEGWQ
                     GGKDGYVIKDETIVSQPRGSGNLYTAKEYGDFHLKFEFKLTPGANNGIGIRTPLTGDP
                     AYVGMEIQVLDDTSDRYKGLKDYQHHGSVYGVVPAKTGHLKPAGEWNSEEIILKGKQI
                     KVILNGETIVDADLEKASTPKTIDGKEHPGLKRDKGHICFCGHGAEVAFRNLRIKSL"
     gene            2664..3470
                     /locus_tag="VFB80_22865"
     CDS             2664..3470
                     /locus_tag="VFB80_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN36697.1"
                     /translation="MQRSGYSQLAAIAVALLLVLGISRQSPRLAAQEPAAAAPLAEAA
                     AAPRKSMLAVIRDGGPLMYPLALCSVALLVFVFERLVSLRRGRVIPRPFVKRFLEQLR
                     DGQLDREKAIKLCDANGSPVAQVFAAAAKKWGRTSVEVEQAILDAGERVNNQLRKYLR
                     LMNGISQIAPLLGLLGTVIGMIISFNGISSAGDSVAQKEMLAGGIAQALISTAGGMFV
                     AIPALAAYLYFVGRVDQLIIEIDALGQQIVELISADALAESRAAKRAKAA"
     gene            3513..3932
                     /locus_tag="VFB80_22870"
     CDS             3513..3932
                     /locus_tag="VFB80_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN36698.1"
                     /translation="MPLKVSHDDLPAINLTPMIDVVFNLIIFFMVSSSFADAEKQVDL
                     TVPQVADSGALRATSARKTINVHRDGRITLDGRSATLSELTSRLAAERRQNVHLNVVL
                     RGDADCPLQHVAGVFTACRKAGVSEMGIAVKLGTKEH"
     gene            3934..>5690
                     /locus_tag="VFB80_22875"
     CDS             3934..>5690
                     /locus_tag="VFB80_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36699.1"
                     /translation="MTQVFARPLLAATYPGVLMIVLAAGLVAIFVVLAVLIWTRFGQA
                     RPISKCLLLSLLAHFLLLVYAYSTHVLFGPLGPGSWNGQTVMMRLRDAADDEEAATVA
                     TPNPEPWEQAGQPDAPLFDATAPSVQAPPAEQLAQSRPVDPPPLLPDAATTTNPAPPA
                     PVTVASESVPIADPMAAEPPAPAAAEAPRAGEQPRESPPTEGTAAADPAGDSPMPPAG
                     AVPAAPSSQFVATAPPVPRRLGDGREVPYPLQARVAAERLKAAVPFGASPQSEAAVAA
                     GLDWLAAAQSGDGRWDADLHGAGRETRTLGHDRRGAGAQADTGVSGLALLAFLGGGET
                     HLEGRHRETVQRGLEFLLASQADSGSVAGHAELFASMYSHGIATLALSEAYALSGDER
                     LLPALRRALQYTIDSQHAGGGWRYQPHDAGDMSQFGWQVMALKSGELGGLAIPPTTRA
                     RMVRFLQSCSSGRNQGLAAYRPGDRASRPMTAEALVCRYFLDAENAPATVAEAAAFVG
                     GERPGIGPANYYYWYYGTLALFQRQGDDWKRWNDALQHELFARQRWDGNAAGSFDPDD
                     LWGGYGGRVYSTALAALSL"
BASE COUNT          947 a         1932 c         1903 g          908 t
ORIGIN      
        1 agcgaagggc atgttcggat cgccgtatca ggggggctat gcgacgcgac atcggccttc
       61 gccgttgcaa atggtggcgg aggttacgaa agcgcccggc cctgaggcga ggaccacagg
      121 aacgctgatg ctgcactatg atccaggcac tcccaggtgc ctcgtgcttg ctccgctcga
      181 cgaattcaat gccgcgctgc tggcgcgcgt tcatccgccc gattggcaga accctacgcc
      241 gcagccgctg tatgatctgg tcgccatcgg cggcgggacc gcgggcctcg tggcggcggc
      301 tggcacggcc ggactgggcg gcaaagtcgc gctcgtcgag cgcggcctgc tgggcggcga
      361 ttgtctcaat ttcggctgcg taccgagcaa agcgctaatc cgcgcggcac gggccgctta
      421 tgacgtcggg cgggccgcgg agttcgggat ccacgtggcg agcggggggc gtcagccccc
      481 tgaggccgcg gcggtcgatt tcgctgccgt catgtcgcgg ctgcgtcgcc tgcgagccga
      541 gatcagcgag aatgactcgg cccagcggtt tcagcagctc ggcgtcgatg tgttcctcgg
      601 cgacgcccgt ttcaccagcc ggcaaacaat tgaggtcgcg ggccagacgc tgcgcttcaa
      661 gcgctgcgtc atcgccaccg gcggccgcgc tgcgattccc gcggtgccgg ggctggagga
      721 ggccggctgc ctgaccaacg agaccgtttt ttcgctcacc gagctgccgc ggcggctgat
      781 cgtcatcggc ggcggaccaa tcggctgcga attggcgcag gcctttcgcc gctttggcag
      841 cgaggtcacg ctcgtgcagc gcggccagcg cctgttgcca aaagacgaac ccgaagcggc
      901 cgagattctg gcccggcaat tcgaacgcga aggaatcaag ctgctatttg gcgccacgtc
      961 ggtccgcgcc gaaacggccg gtggaatcaa gcggctgcta gtgaattcag gcggccgcca
     1021 gcaaggattg gatgccgatg ccgtcctggt cgccgccggc cgaacgccca acatcgaggg
     1081 actaaacctc gcggcggcgg gcgtggaaca caataatcgc ggcgttatcg tggacgactt
     1141 tctgcaaacc gccaatccgc gcatcatggc ggctggcgac gtcgcgggcg gctatcagtt
     1201 cacgcacgcg gccgatgcga tggcccgcgc gtgcgtgcag aacgcattct tctcgctcgc
     1261 cggcttcggc cgcaagcggc tgagcgggct cgtcgtgccc tggacgacct acaccgatcc
     1321 cgagatcgcc cacgtcggcc tgacgcccgc gagcgcggcc gaccagcacc tcgagatcga
     1381 cagctaccgc gaggaactgc ggcacgtcga tcgagcgatt ctggacggcg acgacgaagg
     1441 ctttgccgtc atccactgcc gccgcggcac accgcaggtc gtcggctgca cgatcgtcgc
     1501 ccggcatgct ggcgagatga tcggcgaagt ttcgttgctc atgacagcac ggattccgct
     1561 ctccaagctg gccggcacga tccactgcta tccgacgcag gcggaaatcc tgaagcgtct
     1621 gggtgaccag tacaacaagc gccggctgac gccgcgcgtc gccgatgtcc ttaaggcgat
     1681 tatcggctgg cgctcgtaga gcaggctgcc agcttgctcg ttcccctcgt cgccgcgccg
     1741 gttgctaacg cgtgaaatcg agcgcacaat caccaatcgc cccgccacca tcgcctcaag
     1801 gattctccga tgacacgcca gatcgcattc gtcgccgcag cgctgcttgt gttcgccgcg
     1861 gcctccgctg cccaggacaa gccagccgtg gaagagggct tcacgccgct cttcgacggc
     1921 aagacgctcg aaggctggca gggcggcaaa gacgggtacg tgatcaagga cgagacgatc
     1981 gtcagccagc cccggggcag cggcaacctg tacacggcca aggaatacgg cgatttccac
     2041 ctcaaattcg agttcaagct gacgccgggc gcgaacaacg gcatcggcat ccgcacgccg
     2101 ctcaccggcg acccggccta cgtcggtatg gagattcagg tgctcgacga tacgtcggac
     2161 cgctacaaag gcctcaagga ttatcagcat cacgggtcgg tgtacggagt cgtgccggcg
     2221 aagacgggcc acctcaagcc ggccggcgag tggaacagcg aagagatcat cctcaagggg
     2281 aagcagatca aagtgatcct caacggcgag acgatcgtcg atgccgacct ggagaaggcg
     2341 agcacgccca aaacgatcga cggcaaggaa cacccgggcc tgaagcgcga caaaggccac
     2401 atctgcttct gcggccacgg cgccgaggtc gcgttccgca acctgcggat caagagcttg
     2461 tagtgcaggt tgcaccaact ggcgaatata ttgcatcccc gtcggtacgc cttatttcgt
     2521 gcctgccgag tgtcgatgct gaagatgcga agcgttagcg gaggccggcc gttgtggctg
     2581 cgcggacccc gcagctagct ttcgcccgct ggcaagggac tgccaggtga gcggtggcct
     2641 cgggcggctg ccgcggagca tcaatgcagc gctcagggta ttcgcagctc gccgccatcg
     2701 cggtcgcgct gctgcttgtg ctgggcatta gccggcaatc gccgcgactg gcggcgcagg
     2761 agccagcggc cgctgctccg ctggccgagg cggccgccgc tccgcggaag agcatgctgg
     2821 ccgtcatccg cgacggcggc ccgttgatgt atccgctggc gctgtgctcg gtcgctcttc
     2881 tcgtatttgt gttcgagcgg ctggtctcgc tgcgacgcgg ccgggtgatc ccgcggccct
     2941 tcgtcaagcg gttcctcgag cagctccgcg acgggcagct cgaccgcgag aaggccatca
     3001 agctctgcga cgccaacggc agcccggtcg cccaggtctt cgccgcagcg gcgaagaaat
     3061 ggggacgcac ttcggtcgag gtcgagcagg ccatcctcga cgccggcgag cgggtcaaca
     3121 accagctccg caagtacctg cggctgatga acggcatttc gcagatcgcc ccgctgctgg
     3181 gccttctggg cacggtgatc ggaatgatca tctcgttcaa cggcatctcg tcggcgggcg
     3241 actcggttgc gcaaaaggag atgctcgccg gcggcatcgc ccaggcgctg atttccaccg
     3301 ccggcggcat gtttgtcgcg attcccgccc tggccgcata cctgtacttc gtcggccgcg
     3361 tggaccagct gattatcgag atcgacgccc tcggccagca gatcgtcgaa ctgatctcgg
     3421 cggacgcgct cgcggagagc cgggcagcga agagggccaa agcggcgtag ccgaaatctg
     3481 caatctgaaa atctgcaatc tgagattccg ctatgcccct caaagtcagc cacgacgatc
     3541 tgccggcgat caacctgacg ccgatgatcg acgtcgtgtt caacctgatc atcttcttca
     3601 tggtcagcag cagctttgcc gacgccgaga agcaggtcga tctgacggtg ccgcaagtag
     3661 cggattccgg cgcgctccgc gccacctccg cacgcaagac gatcaacgtg caccgcgacg
     3721 gccggatcac gctcgacggc cggtcggcca cgctgagcga gctgacctcc aggctggctg
     3781 cggagcgccg gcagaacgtg catctgaacg tcgtcctccg cggcgacgcc gactgcccgc
     3841 tccagcacgt cgccggcgtg ttcactgcct gccggaaggc gggcgtctcc gaaatgggga
     3901 tcgccgtgaa actgggaacc aaggagcatt gacttgacgc aggtcttcgc gcggccgctc
     3961 ttggccgcga cctatcccgg cgtcctgatg atcgtcctcg ccgcggggct ggtcgcgatc
     4021 ttcgtggtgc tcgccgtact gatctggacg cggttcggcc aggcgcgccc gatctccaag
     4081 tgcttgctgc tctcgctgct ggcccacttc ctgctcctgg tctatgccta cagcacgcac
     4141 gtcctcttcg gcccgctcgg ccccggcagc tggaacgggc agaccgtcat gatgcggctg
     4201 cgcgacgccg cagacgacga ggaggccgcc accgtagcca cgcccaatcc agagccctgg
     4261 gagcaggccg gacagccgga tgctccgttg ttcgatgcca cggcccccag cgttcaggca
     4321 ccaccagccg agcaattggc gcagtccagg ccggtcgatc cgccgccgct gctgccagac
     4381 gctgcaacga cgaccaatcc tgcgccgcca gcccccgtaa ctgtcgcgag tgaatcggtg
     4441 ccgattgccg atccaatggc cgccgagccg cccgcgccgg cggccgccga agcgccgcgt
     4501 gcaggcgaac agccgcgaga gagtccgccc accgaaggca ccgcggccgc cgatcccgct
     4561 ggcgattcac caatgccgcc ggccggcgct gtacctgcgg ctcccagcag ccagttcgtg
     4621 gcgaccgcgc cgcccgttcc gcggcggctg ggagatggcc gcgaagttcc ctatccgctg
     4681 caagcccgtg tcgccgccga acggctgaag gccgccgtgc catttggcgc ctcgccgcag
     4741 tccgaagccg cggttgccgc ggggctcgac tggctggccg ccgcgcagag cggtgacggc
     4801 cgctgggacg ccgacctgca cggcgctggc cgcgagaccc gcacgctcgg ccacgaccgc
     4861 cgcggcgcgg gcgcacaagc cgacaccggc gtcagcggtc tggctctgct cgcgtttctc
     4921 ggtggcggcg aaactcattt ggagggacgc catcgcgaga ctgttcagcg cggcctggaa
     4981 tttctgctcg ccagccaggc cgacagcggc agcgttgccg gccacgccga gctgttcgcc
     5041 tcgatgtact cccacggcat cgccacgctg gccctcagcg aggcgtatgc gctctccggc
     5101 gacgagcggc tgctgccggc actgcggcgg gccctgcagt acacgatcga ctcgcagcac
     5161 gccggcggcg gctggcgcta tcagccgcac gacgccggcg acatgagcca gttcggctgg
     5221 caggtgatgg cgctcaagtc gggcgagctg ggcggcctgg cgattccgcc aacgacgcgg
     5281 gcgcgaatgg tgcggtttct gcaaagctgc tcctcgggac gcaatcaggg tttggcggcg
     5341 taccggccgg gcgaccgcgc tagccgaccg atgacggccg aggcgctggt ctgccgctat
     5401 ttcctggacg ccgagaacgc gccggcgacc gtggccgagg ccgcggcatt cgtcggcggc
     5461 gagcggccgg gcatcggccc ggcgaattat tactactggt attacggaac gctggcgctg
     5521 tttcagcggc agggggacga ctggaagcgc tggaacgacg ccctgcagca cgagctgttc
     5581 gcgcggcagc ggtgggacgg caacgcggcc ggcagtttcg atccggacga cctgtggggc
     5641 ggttacggcg gccgcgtcta tagcacggcc ctcgccgccc tctcgctcga
//
LOCUS       DASRJB010000871         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2781604, whole genome shotgun sequence.
ACCESSION   DASRJB010000871 DASRJB010000000
VERSION     DASRJB010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781604"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFB80_22880"
     CDS             <1..370
                     /locus_tag="VFB80_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36700.1"
                     /translation="PSSVGQEAKKSDKANKTETKAKGRLPAYYADIVTEEQREKIYAI
                     QAKHVEKISELNAQLTAATKARDTEIENVLTAEQKVKLKAAQEEAAAKKKKAGTEKKA
                     AEDGKKAAPAAEAKTTTKTK"
     gene            complement(376..1395)
                     /locus_tag="VFB80_22885"
     CDS             complement(376..1395)
                     /locus_tag="VFB80_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZN36701.1"
                     /translation="MPRSSSLLFPLWIACAAGCWSGRGGEVVVYAALDREFSEPVLAE
                     FSKTSGVKALAKYDDESTKSVGLTSILIQEASRPRCDVFWNNEILNTLRLEKRGLLEA
                     YQSPAAAAFPAMFRSASGTWCGFAGRARVLIVNTRLVSEDQRPKSIYDLADSKWKGQI
                     GIAKPVAGTTATHAAVLFAVLGDEKAKEFFRSLKANQVQILGGNKQVAEACTAGRIAF
                     GLTDSDDAIIEIERAGPVAIVYPDRRADQLGTLFIPNTAAIIKGCPHPAAARRLVDYL
                     LSPAVEANLAAGRSAQIPLNPNVLVKPRVETPATVKALPADFAAAADKWDAAAVFIEQ
                     EFLAP"
     gene            1479..1877
                     /locus_tag="VFB80_22890"
     CDS             1479..1877
                     /locus_tag="VFB80_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36702.1"
                     /translation="MARHRLLLLVAALLAPALGWQVWLAGQQPAARTQPASNRFEFEV
                     IQSYDARYQGDTPGHMGRSGGLENRKLQVALGDPVFRGEQQVGKVTGLGWSRPHGSLE
                     VEFDPLPAVRVCVGDSVWIALDGQKPAVSR"
     gene            1951..3654
                     /locus_tag="VFB80_22895"
     CDS             1951..3654
                     /locus_tag="VFB80_22895"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36703.1"
                     /translation="MTHLDPVIDTAAGAAARPRAALEAGVQIGDFRIERRLGAGGMGI
                     VYLARQLSLDRLVALKVLGQALSRGTDVKRFQREAQAAARLKHPSIAQVFYIGQDRHV
                     CYLAMELIDGVSLRTALDRLGTATSVEASLDTAVTEELGDNPVAPIVRFDVPTVDSDR
                     PPGGSDRAPASDSGQLNPYLSPRARELRTNQAHVRRVCEVIRDAAQALAHAHEQGVVH
                     RDIKPDNLLLDRRGRVHVIDFGVARFFDDQSVTYTGQLVGTPLYMSPEQVTGRGAIDS
                     RTDIYSLGLVLYELLALRVPVEATNRENLLRTIVTKSLPPISWRNSAVNSELERIVHR
                     ATQKDPDQRYASASEFAADLENYLQGQPITAPPYHYRLDEREITAARPSEVVSAAVIF
                     FVLAFFLFTSIESVSLMMFFMLPGMSGVVMCAIQSGIALTCLLLGSAIGSGVLAAQTW
                     ARWTGVAISSLLATVSLVGIVMVVIAVFAALNNDFGPVGLDPEPAAQGGAAPAGGPPF
                     SMRNMVLGMIAMYSLPIVMGLALGGSALWCLLCRATGRWFRFARDLRLEHRAAKEQLA
                     A"
     gene            complement(4148..4576)
                     /locus_tag="VFB80_22900"
     CDS             complement(4148..4576)
                     /locus_tag="VFB80_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HZN36704.1"
                     /translation="MATATYHPVAARPNTHSIAVGYICWLFGFLGLHRFYYGKPISGT
                     IWFFTLGLLGIGWLIDLFLIPSMDAKADRRYVPGRLDYNLAWILLAVPPLGWLGLHRF
                     YMGKVVTGIIWLCTGALVGIGWLYDLWTLNDQVSEINARG"
BASE COUNT          865 a         1570 c         1546 g          801 t
ORIGIN      
        1 gccgtcgtcg gtcggtcagg aagccaagaa gtcggacaag gccaacaaga cagagaccaa
       61 agcgaaaggc cggctccccg cctactacgc cgatatcgtg accgaggaac agcgggagaa
      121 gatttatgcg atccaagcaa agcacgtcga gaagatttcg gagctcaacg cccaattgac
      181 ggccgcgaca aaggcgaggg acacggaaat cgaaaacgtg ctcaccgccg agcagaaagt
      241 aaagctcaaa gctgcccagg aagaagccgc tgccaagaag aagaaagccg gcacggagaa
      301 gaaggcggct gaggacggca agaaggccgc gcccgccgcg gaagccaaga caacgacgaa
      361 aacaaagtag cgccgctagg gcgcgaggaa ctcctgctcg atgaacactg ccgccgcatc
      421 ccacttgtcg gcagcggcgg cgaagtcggc aggcagagct ttcacagtgg ccggcgtttc
      481 gacacgcggc ttgaccagca cgtttgggtt gagcggaatc tgcgcgctgc ggccggccgc
      541 cagattggct tccaccgccg gcgacaggag atagtcgacg agccgcctgg cggcagcggg
      601 atgagggcag cccttgataa tcgctgccgt attgggaatg aacagcgtgc cgagctgatc
      661 ggcccggcgg tcgggataga cgatcgccac ggggccggct cgctcgattt cgataatcgc
      721 gtcatcggag tctgtcagcc cgaaggcgat tcggccggcg gtgcaggctt cggccacctg
      781 cttgttgccg cccaggatct gcacctgatt ggctttcagg ctgcggaaga actccttggc
      841 cttctcgtcg cccagcacgg cgaagagcac ggcggcgtgc gtcgccgtcg tgcccgcaac
      901 gggcttggcg atgccgatct ggcccttcca tttcgagtcg gccagatcgt agatcgactt
      961 cggccgctgg tcttcggaca cgagcctggt attgacgatc aagacccgcg cccgcccggc
     1021 gaagccgcac cacgttccgc tggcagagcg aaacatcgcg ggaaacgcgg ctgccgccgg
     1081 cgactggtag gcctccagca gtcctcgctt ttccagccgc agcgtattga gaatttcgtt
     1141 gttccagaac acatcgcacc gcgggcggct ggcctcctgg atcaggatcg aggtcaggcc
     1201 gaccgacttc gtgctctcgt cgtcgtactt tgccagcgcc ttgacaccgc tggttttcga
     1261 gaactcggcc agcaccggct cggagaactc gcgatcgagc gcggcgtaga cgacgacctc
     1321 gccgccgcgg cccgaccagc agccggcggc gcaggcgatc caaagcggaa acaacaggct
     1381 agacgatctt ggcatgcatc ctcgccaggc aggcgcacga ccggaaatga tacgatggag
     1441 cgcgccgctc gccaccgctc gcacaaggag attatcgcat ggcacgccat cgcttgctgc
     1501 tgctcgtcgc tgctttgctg gccccggcgc tgggctggca agtctggctc gctggccagc
     1561 agcccgccgc tcggactcag ccggcgagca accggtttga gttcgaagtc attcagagct
     1621 acgacgccag ataccagggg gacacgcccg ggcacatggg ccgctcgggc ggactggaga
     1681 accgcaagct gcaagtggcg ctgggcgacc ccgtgttccg cggcgagcag caggtcggca
     1741 aagtcacggg gctcggctgg agccggccgc acggcagcct ggaggtggaa ttcgacccgc
     1801 ttcccgcggt gcgggtttgc gtcggcgaca gcgtttggat cgcgctcgat gggcaaaagc
     1861 cggctgtttc gcggtagaaa cgccgcgaca cttccggttt gctcgaaggg tagcgataat
     1921 tggcggcgga ctgatccccc agccgaaatc atgacgcacc tcgaccccgt gatcgacacc
     1981 gccgctggcg cagccgcccg acctagggcc gcgctggaag cgggcgtgca gattggcgac
     2041 ttccgcatcg agcggcggct aggcgcgggg ggcatgggca tcgtctatct cgcccggcag
     2101 ctttcgctgg accggctcgt cgctctgaaa gttctgggcc aggccctcag ccgcggaacc
     2161 gacgtgaagc ggtttcagcg cgaggcgcag gcggcagccc ggctgaaaca cccttcgatc
     2221 gcgcaggtct tctacatcgg ccaggaccgc cacgtctgct acctggcgat ggagctgatc
     2281 gacggcgttt cgctgcgcac ggcgctggac cggctgggga cggccaccag cgtcgaggcg
     2341 agcctggaca cggccgtcac cgaggaactg ggagacaacc cggttgctcc gattgtgcgg
     2401 ttcgatgtgc cgacggtcga ttcggaccgc ccgcccggcg gcagcgatcg cgcgccggcc
     2461 tccgattcgg gccagctcaa tccctatctc tcgccgcggg cccgcgagct gcggacgaac
     2521 caggcccacg tccgccgcgt gtgcgaagtc atccgcgacg cggcccaggc gctcgcccac
     2581 gcccacgagc agggcgtggt tcaccgcgac atcaagcccg acaacctgct gctcgaccgc
     2641 cgcgggcggg ttcacgtgat cgatttcggc gtggcccggt tcttcgacga ccagtcggtg
     2701 acctatacgg gccagctcgt cggaacgccg ctctacatga gccccgagca agtcaccggc
     2761 cgcggggcca tcgactcccg caccgacatc tattcgctgg gcctggtgct ctacgagtta
     2821 ttggccctcc gcgtgccggt ggaagcgacc aaccgcgaaa acctgctgcg gacgatcgtc
     2881 accaagtcgc tgccgcccat ctcgtggcgg aactccgccg tcaacagcga gctggagcgg
     2941 atcgtccacc gcgcgacgca gaaggacccc gatcagcggt acgcctcggc cagcgagttc
     3001 gcggcggacc tggagaacta cctccagggt cagccaatca ccgcgccgcc gtaccattat
     3061 cgcctcgacg agcgcgagat cacggccgcc cggccgagcg aagtcgtctc ggcggcggtg
     3121 atcttcttcg tgctggcgtt cttcctgttt acttcgatcg agagcgtctc gctgatgatg
     3181 ttcttcatgc ttcccggcat gtccggcgtc gtcatgtgcg ccatccagtc gggcatcgcg
     3241 ctgacctgcc tgctgctcgg gtcggcgatc ggttcgggcg tcctcgcggc gcagacttgg
     3301 gcccgctgga ccggcgttgc catctccagc ttgctggcga cggtatcgct cgtcggcatc
     3361 gtgatggtgg tgatcgcggt cttcgccgcg ctcaacaacg atttcggtcc tgtcggtctg
     3421 gatccggagc cggccgcaca gggcggggct gctcccgctg gcggccctcc gttttcgatg
     3481 cgcaacatgg tgctgggcat gatcgccatg tactcgctgc cgatcgtgat gggactggcg
     3541 ctgggcggct ccgccctgtg gtgcttgctg tgccgcgcca ccggccgctg gttccgcttc
     3601 gcccgcgact tgcggctgga gcaccgcgcc gcgaaggagc agttggccgc ctgatgcacc
     3661 agcgccggcg aatcttcgcc gggtcgggca gctccgtggc gaataattcg gccgcgattg
     3721 gcgagattcg caggcttttt ccgcactttt gacggcgaat attcgccaat ttgccccgat
     3781 acctgcggat ctggcgatta attcgccgcc gccggccgga agtgccttgt ggcatcggca
     3841 gttacgagcg aaatgagggt ggcgaataat tcgccgcctc gccgcaccct ctgatcgcaa
     3901 ctggcgcccg atggcgcgca gactcacggc accacgcgcg accagccgcg tcgcgacctt
     3961 ccacgaattt ccaaagatca tgtcgccgcg cctgggcccg gcaggcgttc ggaattgtaa
     4021 ctatccgtcc agtatctgtc aagtcaacca caacagattg tggtcgcctt ccgccaaacc
     4081 acaataggtt gtggtcggcg cacgaagagg cccccgaaag agggcccgcc attttcccag
     4141 ggtctgttta gccacgggcg ttgatttcgc taacctggtc gttcagcgtc cacagatcgt
     4201 aaagccagcc gatgcccact agagcccccg tgcagagcca gatgatgccg gtaacgacct
     4261 tgcccatata gaaccggtgc agcccgagcc aacccagcgg cggcaccgcc agcagaatcc
     4321 aggccaggtt gtaatcgagc cggccgggca cgtagcggcg gtcggccttg gcgtccatcg
     4381 acggaatcag gaacaagtcg atcagccagc caatgcccaa cagacccagc gtgaagaacc
     4441 aaatcgttcc gctgatgggc ttgccgtaat aaaaccggtg cagccccaga aagccgaaca
     4501 gccagcagat gtagccgacg gcgatcgagt gcgtgttggg gcgggcggca accgggtgat
     4561 aggtagcagt ggccatagct actcattcgc tggccggccg acagtattgg cggcacgagt
     4621 gctgccggcc tgacgggaaa gcactgggtc gttagcgatc tactcttcgc tgccagcttt
     4681 cgtgtcgcgc ccttagtgat cgttcgtgga tataaggaaa acggaccgcc ccaggctgct
     4741 tctcgccgcg ccccgctgcg ccgccgctcg cgccgcaact gc
//
LOCUS       DASRJB010000872         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_257637, whole genome shotgun sequence.
ACCESSION   DASRJB010000872 DASRJB010000000
VERSION     DASRJB010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257637"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="VFB80_22905"
     CDS             complement(<1..1958)
                     /locus_tag="VFB80_22905"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36705.1"
                     /translation="MAVLRPFLVLSVLSLAASAPADDAALTPAQEKFFEEKVRPVLAT
                     NCQECHGAKKQESGLRLDSRAAVIEGGDSGERAAVPGEPERSLLVKAINHVGEYHMPP
                     NRKLTGEQIAALTEWVKDGLPWPAAVTPAAEHKLSAAELVPLHRQSHWAYQPVNRQPL
                     PSVGEAAWLRMRTDAFILAKLDSAQLTPSPEADRRTLIRRLSFDLIGLPPMPEEVETF
                     AADESPDAYERLTDRLLASPRYGERWGRHWLDVVRYADTKGYAFAQERRFPYAYTYRD
                     YVIRALNRDLPYDQFLIEQLAADRLPSDDMTTLAALGFLTTGRKFNNRNDDIDDQIDA
                     VARGMLGITVACARCHDHKYDAIPTEDYYSLYGVFASCSEPGELPLIAPPEQSAEYQK
                     FEEELGKIRGEFDKFVTEAHVAFLDQARHQAADYLARVAAGESQSLLAKLPFLSLDPK
                     DLRPRMIDRWRRYLEEHAKPDHTALGLWHDLLKVSTENFAASSKPVLARWQSLAAGTA
                     AGQCNPVIQQAFAADVPATKVDVARVYGRLLTDAYAQWQAAGGNAEALGKLQAEQRQL
                     AEILLGKGAPTDIERGEVRQYLNRADRNKYQELQKKVESFQATSPLAPPRAMVVADKP
                     QPEEPRVLIRGQPARPGNLVPRQFLLVLS"
     gene            complement(2078..3364)
                     /locus_tag="VFB80_22910"
     CDS             complement(2078..3364)
                     /locus_tag="VFB80_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263149168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HZN36706.1"
                     /translation="MRILLSLGLALLTFGAAHGQQSQLGESLKDIPVADHWIYDDFPK
                     AVAEAKASNKPLLVVIRCVPCPPGKTLDVAVMTPDQKLAELEKKFVCVRLIQTNNLDL
                     SLFQYDYDMSWASMFLTPDLAVLGRYGTRAGNQANSDTYLSAAGFANAAERALELYRA
                     YPGNKAELAGKRGPQPQYTSANQIPGLTDRPAVASVRQNCVHCHMLKEFALRAKWEAG
                     KLTKEDLYVYPLPQSIGLTIDKEDGLLVSSVAAGSPAEKAGIKSGDALAAIGTQPLIS
                     MADIQWALNSAPSEGALNVKVVRGGQPLVKSLALSGDWKKSDIAWRASSWYGLRHGVK
                     LDPLPVADKEKRGIGADQMALVVKNIYAPQGKPHAAIKAGLKPNDVIVAVNGKSDATN
                     ESDFFVNLRLSHGPSDSVKLTILRGNERQELTVPLW"
     gene            complement(3417..3914)
                     /locus_tag="VFB80_22915"
     CDS             complement(3417..3914)
                     /locus_tag="VFB80_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36707.1"
                     /translation="MAAEPVKSGLQPGEKITTIFEPLNVTGEHAGEPYCLICENGLAP
                     VAMLFAREVNEELLSLLGKIDAATAKNRDKQMGSFVVFLSEKQDLRQQLVDAAKRRGF
                     KHIVLSIDSPAGPEGFKVSGDADLTVVLYREHDVKANHAFRKGELNAAAAEKVLADLP
                     IILRP"
     gene            complement(4076..5266)
                     /locus_tag="VFB80_22920"
     CDS             complement(4076..5266)
                     /locus_tag="VFB80_22920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN36708.1"
                     /translation="MSTASPPLPPGGFVPKFHPRILGSAAPVFPGFEVDILDRVPDDE
                     RQDAVWHRERAKAMLKAHPEIKSLFGNDPATAFWCLAAASTQLGIAVAVGYAPWWVML
                     LAAWLVGSSINIMLFQLGHECVHGLVFQRKSWNRVMFTLTTLPMFLSGHHTWWEEHLV
                     HHNDMGAKKDFITRRRSYFLATRLTSPLVVPYSLLMLVMQVLRSTLGLLMYCGGLLLG
                     HFKPTPRTLAVLADEHLVSGYRKDGSEMWAVCYPLLNLTTCGMLLLLGGWQSVAYLLA
                     SQAFFTGFLHPYCLGWVLGISHFHGARRYQPTASHYGWLINLLSFNAGYHVEHHDLMT
                     IPWRQMPALRRMAPEFYDNLEPIRSYTWLAMQFVFAWPQYFEANFNHEAHRNAARFQG
                     EQAA"
     gene            complement(5348..5872)
                     /locus_tag="VFB80_22925"
     CDS             complement(5348..5872)
                     /locus_tag="VFB80_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36709.1"
                     /translation="MPALDHLAGLFYSDMAELGAFEEVLAEQLPQPYRTLLAHHEHMT
                     VAVEKYHSEKVEVEVIAARRTGDYYCRKIVLHLQSDAKTVLFGIPRLNLRLLDDDVRR
                     EILSENKPLGRVLIDHNVLREVQLASLYRVTAGPDLCRMFGLAEPLPTYGRTAFIYCD
                     GYPAVELLEIVSPS"
BASE COUNT         1106 a         1915 c         2008 g         1095 t
ORIGIN      
        1 gacagcacga gcagaaactg ccgcggcacc agattgcccg gccgggcggg ttgtccgcga
       61 atcaagaccc gcggctcttc cggctggggc ttgtcggcca caaccatcgc ccgcggcgga
      121 gccagcggcg aggtggcctg gaacgattcg actttctttt gcagctcctg gtacttattg
      181 cgatcggccc ggttgaggta ctgccgcact tcgccgcgct cgatgtcggt gggcgcgccc
      241 ttgcccagca gtatttcggc cagttgccgc tgctcggcct gcagcttgcc cagagcctcg
      301 gcattgccgc cggcggcttg ccactgggca taggcatcgg tcagcaggcg gccataaacc
      361 ctcgccacat ccaccttggt cgccggcacg tcggcggcga acgcttgttg gatcacgggg
      421 ttgcactggc cggcggcggt gccggccgcc agcgactgcc acctggccaa tacgggcttg
      481 gaactcgcgg cgaaattctc ggtggagacc ttcagcaggt cgtgccacaa gccgagcgct
      541 gtgtgatcgg gtttggcatg ctcctcgaga taccgccgcc agcggtcgat catccgcggc
      601 cgcaggtctt tgggatcgag cgacagaaac ggcagcttcg ccaagagcga ctgcgactcg
      661 ccagcggcga cgcgggccag gtagtcggcc gcctgatgcc gcgcctggtc gagaaacgcc
      721 acatgggcct cagtgacgaa cttgtcgaac tcgccgcgaa tcttgcccag ctcttcctcg
      781 aacttctgat actcggcaga ttgctccggc ggggcgataa gcggcagctc gccaggctcc
      841 gagcagctcg cgaagacgcc gtacaacgaa tagtagtctt cggtcggaat cgcatcgtat
      901 ttgtggtcgt ggcagcgggc gcaggcgacc gtgatgccca gcatgccgcg ggccacggcg
      961 tcaatctggt cgtcgatgtc gtcgttgcga ttattgaatt tgcggcccgt cgtgaggaat
     1021 ccgagggcgg ccagcgtcgt catgtcgtcc gacggcaggc gatcggcggc gagttgctcg
     1081 atcaagaact ggtcgtaggg cagatctcgg ttgagcgccc ggatgacgta gtcgcggtag
     1141 gtgtaggcat acgggaagcg ccgctcctgc gcgaaggcat agcccttcgt gtcggcgtag
     1201 cggaccacgt cgagccagtg gcggccccac cgctcgccgt accgcggcga ggccagcagc
     1261 cgatcggtca atcgctcgta ggcgtcgggc gactcatcgg cagcgaacgt ctcgacttcc
     1321 tccggcatcg gcggcaagcc gatcagatcg aagctcagcc ggcggatcag cgtgcggcgg
     1381 tcggcctcgg gcgacggcgt cagctgcgcg gaatcgagtt tcgccaggat gaaagcgtcc
     1441 gtgcgcatgc gcagccaggc ggcctcgccg acactcggca gcggctgacg attcaccggc
     1501 tgataggccc agtgcgattg gcggtgcagc ggaacgagct ccgctgccga gagcttgtgc
     1561 tccgcggcgg gcgtgacagc agccggccag ggaagcccgt ccttgaccca ctccgtcagc
     1621 gccgcgattt gctctcccgt gagcttgcgg ttgggcggca tgtgatactc gcccacgtgg
     1681 ttgatcgcct tgaccagcaa gctccgctcc ggctctccgg gcacggccgc ccgctcgccg
     1741 ctgtcgcccc cctcaatcac ggccgcacgt gagtcgagcc gcaagccgga ctcttgcttt
     1801 ttcgcgccgt ggcactcctg gcaattcgtg gccaggaccg ggcggacttt ctcctcaaag
     1861 aacttctcct gcgcgggtgt gagggccgca tcgtcggcgg gcgcgctggc tgccaacgac
     1921 aggaccgaga ggacgaggaa cggacgcagc acagccatcc ggcacctgca ggcaggaacg
     1981 atcgcggcgg gaagcgcgcg agcacgctcc tcaaccagta gaaatcaaat ccgcagcgcc
     2041 agcaacccaa aaccggcgac cgccatacag ccgcggctta ccacagcggg accgtcagct
     2101 cctgccgctc gttgccgcgg agaatcgtga gcttcaccga gtcggacgga ccatgactca
     2161 gccgcaagtt gacgaaaaag tccgactcgt tcgtcgcgtc cgacttgccg ttgaccgcga
     2221 caatgacgtc gttgggcttg aggccggctt tgatggcggc gtgcggctta ccctgaggag
     2281 catagatgtt cttcacgacg agggccattt gatccgcacc gattccgcgt ttttccttgt
     2341 cggccaccgg gagcggatcg agcttgacgc cgtgccgcag gccgtaccac gaggaggccc
     2401 gccaggcgat atctgacttc ttccaatcac cgctgagcgc cagcgacttg accagcggct
     2461 gaccgccgcg aacgaccttg acgtttagtg cgccttcgct gggcgcggag ttgagcgccc
     2521 actggatgtc ggccatcgag atcagcggct gcgtgccgat cgccgcgagc gcgtcgccgg
     2581 attttatgcc cgccttctcg gccggcgagc cggcggcgac cgaactcacg agcaggccgt
     2641 cttccttgtc gatcgtcagg ccaatgctct gcggcagcgg atagacgtac aagtcttcct
     2701 tcgtcagctt gccggcttcc catttggccc gcagggcgaa ttccttgagc atgtggcagt
     2761 gcacgcagtt ttgccgcaca ctggccacgg cgggccggtc ggtgaggccg ggaatttgat
     2821 ttgcgctggt gtactgcggc tgcggccccc gcttgccggc cagctcggcc ttgttgccgg
     2881 gataggcgcg gtacagttcc agcgcccgct cggcggcgtt ggcgaatccg gcggcggaga
     2941 gatacgtatc ggagttggcc tggttgccgg cccgcgtgcc gtagcggccg agcaccgcca
     3001 ggtcgggcgt gaggaacatc gacgcccacg acatgtcgta gtcgtactgg aacaggctca
     3061 ggtcgagatt gttggtctgg atcagtcgga cgcagacgaa tttcttctcc agctcggcta
     3121 gcttttggtc cggcgtcatc acggcgacgt cgagcgtctt gcccggcggg cagggcacgc
     3181 agcggatcac gaccagcaat ggcttattgc tcgctttggc ctcggcaact gccttgggaa
     3241 agtcgtcgta gatccagtgg tcggcgacgg gaatatcctt gagcgattcg cccagctggg
     3301 attgttggcc gtgggctgca ccgaacgtca acagtgccag accgagagac agcaggatac
     3361 gcacgtggga tgctccgggt aggatggggt gggcgcgagc aagcgaggac gctgctctac
     3421 gggcggagga ttatcggcag atcggcgagg accttctcgg cggcagccgc gtttagctcg
     3481 cccttgcgga aggcgtggtt ggctttcacg tcgtgctcgc ggtagagcac gacggtgagg
     3541 tcggcgtcgc cagagacctt gaagccctct gggccggcgg gcgaatcgat gctcagcacg
     3601 atgtgcttga agcctcgccg cttcgccgcg tccacgagtt gctgccgcag atcttgcttt
     3661 tcgctgagga acacgacaaa gctgcccatt tgcttatcgc ggttcttcgc ggtggccgcg
     3721 tcgatcttgc cgagcagcga cagcagctcc tcgttcacct cgcgggcaaa cagcatcgcc
     3781 accggcgcga gcccgttctc gcagatcagg cagtacggct cgccggcgtg ctcgcccgtc
     3841 acgttgagcg gctcgaagat ggtggtgatc ttctcgcccg gctgcaggcc cgacttcacc
     3901 ggctcggcgg ccatgagcca accgccacag aataacaaag ccggcatagc tgccagcgcg
     3961 aagcgcctca tcgatcgtct ccgctaactg aacgcctgga atgacaaatt gtgagcctaa
     4021 cagccgccag cgggctgtgc aacggctaaa acagtagacc gcgtagcgca ccgagctagg
     4081 cggcttgctc gccttgaaag cgggcggcgt tgcggtgggc ctcgtggttg aaattggcct
     4141 cgaagtactg cggccaggcg aagacaaact gcatcgccag ccacgtgtag gaccggatcg
     4201 gctccaggtt gtcgtagaac tctggcgcca tccgccgcaa ggccggcatc tgccgccacg
     4261 ggatcgtcat caagtcgtgg tgctcgacgt gatagcccgc attgaacgac agcaggttga
     4321 tcagccagcc gtagtgcgaa gccgtcggct gatagcgccg cgcgccgtgg aagtgcgaaa
     4381 tacccagcac ccagcccagg cagtaggggt gcagaaaacc ggtgaagaac gcctggctgg
     4441 cgagcagata ggcgaccgac tgccagccgc cgaggagcag cagcatgccg cacgtcgtca
     4501 ggttgagcag cggataacag acggcccaca tctcggagcc gtccttgcgg tagccggaga
     4561 cgaggtgctc gtcggccagg acggcgagcg ttcgcggcgt cggcttgaaa tggcccaaga
     4621 gcaggccgcc gcagtacatg agcagcccga gcgtgctgcg caggacttgc atcacgagca
     4681 tcagcagcga gtagggcacg accagcggcg acgtcagccg cgtggccaga aagtacgagc
     4741 gtcggcgggt gatgaagtcc ttcttggcgc ccatgtcgtt gtggtgcacc aggtgctctt
     4801 cccaccacgt gtgatgcccg gagaggaaca tgggcagcgt cgtgagcgta aacatcaccc
     4861 ggttccacga cttccgctga aagaccagtc cgtgcacgca ctcgtggccg agctgaaaga
     4921 gcatgatgtt gatcgacgac ccaaccagcc aggccgcgag cagcatcacc caccaggggg
     4981 cgtagccgac ggccaccgcg atgcccagtt gggtgctggc ggcggccagg caccagaaag
     5041 ccgttgccgg atcgttgccg aacagcgact tgatttcggg atgggccttg agcatcgcct
     5101 tggcccgctc gcgatgccac acggcatcct gccgctcgtc gtcgggcacg cggtcgagaa
     5161 tatcaacctc aaagccggga aacacgggcg ccgcgctgcc caagatgcgc ggatgaaatt
     5221 tcggtacaaa gccgccggga ggcagcgggg gagatgcggt gctcatgaca cctcgctagc
     5281 aggtacgacc ggaaaggggg gactttacgg agcgtatgct ttgcaggcta ggtgagttct
     5341 agcgttctta cgagggcgat acaatttcca ataattctac ggctggatag ccgtcgcaat
     5401 agatgaacgc cgtccggccg taagtcggca agggctcggc gaggccaaac atcctgcaca
     5461 aatcgggccc agccgttacg cgatacagcg aggccagctg cacttcgcgg agcacgttgt
     5521 ggtcgatcag cacgcgcccc aacggtttgt tttcgcttag aatctcccgc cgcacgtcgt
     5581 cgtcgagcag ccgcaggttc agtcgcggga tgccgaacaa aaccgtcttc gcgtcggatt
     5641 gcaagtgcag gacgatttta cggcagtaat agtcgccggt ccgcctggcg gcgatcacct
     5701 cgacctcgac cttctcactg tggtacttct cgactgcgac ggtcatgtgc tcgtggtggg
     5761 ccagcagcgt ccgatacggc tgcggcaact gctcggccag cacttcctca aacgcgccca
     5821 gctccgccat gtcggagtag aacaggccgg caaggtgatc gagagcgggc atcgagcaaa
     5881 aacatgcggc ggggggactt agccatgaaa aacacgaaaa tccaaacact ggatttcgct
     5941 acgtctctct ttcgcattcg tcttgtgctt ttcgtggcta ctgaatcgaa gtcaacacgg
     6001 tcggcgacgg cgcagcattg gcgtggtcaa ggacgctgtc gatcaggtag tagagcaaag
     6061 cccgcagctc ttcgggctgg atctcgtcgg caatcttttc ggcccgctcc tggtgctttt
     6121 cgat
//
LOCUS       DASRJB010000873         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3362539, whole genome shotgun sequence.
ACCESSION   DASRJB010000873 DASRJB010000000
VERSION     DASRJB010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362539"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1207)
                     /locus_tag="VFB80_22930"
     CDS             complement(23..1207)
                     /locus_tag="VFB80_22930"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN36710.1"
                     /translation="MSQPQPIASGPSPPSAPTAPASRPRASWPLIVLAWAAAIAGLYF
                     AQDVMIPVVLAALLALLLRPILRRLRRLNFPDGAAALLLVGCLAAVFVVGVWMLAGQA
                     QHWLAQAPQTVERVRNLLPKRAGPLQDLQQTSDAVQGLTRSDGAEPPVQVEVQSSDLA
                     YALLGVSGKVTAMAVIVFVLGFFLLAFSESLLRHAVTLCTSFAQKRTVVQAVLDVENG
                     ISRYLLTISTINAGLGVATGLVMWALGIPSPLLWGVLAATANYVPHVGAFVCMLVLFF
                     VGAVSHESLGYGLLTAGAFVVLTSAESYFVTPLILSRSLQLSPLAVILAILFGGWLWG
                     IAGIALAMPLVVTAKALIMELALQGADTRASPDATTMRSRAESLLGLGAARHRRPETE
                     RR"
     gene            complement(1280..1759)
                     /locus_tag="VFB80_22935"
     CDS             complement(1280..1759)
                     /locus_tag="VFB80_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZN36711.1"
                     /translation="MAKSSKFWLVKSEPESFSIQDLAKAKDQTTCWDGVRNYQARNFM
                     RDEMKVGDRVLFYHSSTDPPAIVGTAQVVRESYPDASAWNKNDHHYDPASTPENPRWF
                     MVDLRLEQIFKQPLPLDTLRGVAALKDMELLRKGSRLSVQPVRKAEFDAVLKLAGAK"
     gene            complement(2067..2459)
                     /locus_tag="VFB80_22940"
     CDS             complement(2067..2459)
                     /locus_tag="VFB80_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36712.1"
                     /translation="MAHQLTITEQPGYLHFRVTGENTPPTVQAYLAEVHTHCVQRGCT
                     AILVEEDLTGPGLPLVEIYQIVERGGMRNRMLLRWLAYVDVHQHERPGNLRFAETVAV
                     NRGLNMRTFASVDEAAAWLGEMTAPTKP"
     gene            2602..>4508
                     /locus_tag="VFB80_22945"
     CDS             2602..>4508
                     /locus_tag="VFB80_22945"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36713.1"
                     /translation="MAILGRSRCVLLALGLVMWGALAARAQDAGLDPNRLPGIVVDDA
                     AAKLEGTWSKSVHTRPFVGAGYIYSQGGAGQSVKFPVEINEAGPYQVLISYTPGTNRT
                     QKANVLVPTADGPKTFVIDQQARPAGPYCFQPLGELTLEPGKLEIVVSAEGSTKGVVV
                     ADAVQILTPDGFKTYKADFEKNTPKLADALKVDPKKSAAKPVAKAVEKKEEPPQEQPP
                     VFVRKEPAKPRAKLTSEQLDKLMQQYVGGIDNAILVDDEAFLRRVTLDLIGRQPTMAE
                     MTSFLKAATSGRGSEHRAAAVEKLLAVPEFGANWANYWSDVISYRTPSPELTFLNYTP
                     FKIWLGEQFNQNKGWDETTYQVITAIGKVADNPAATYIGFHGGDKSRLAAETTRVFLS
                     TQIQCAECHDHKFVDMPQETFHHIAAFFVRASAKLPWNDSSQIVVSSKPSGEHKMEGK
                     GADMKPIAFSERQVDLGKSDVARRAELADWIVGPENPWFAKAFVNRVWARMMGRGFCE
                     PVDEIGELGDRVLPEVHTALADHFVAGKHDVKDVFRVVALSKSYQREIRDADKGQNKA
                     FVAIPAGRLRGDEVFDSLQAAIALPNVTPKQGEATTAFRFPPPPKSTRDLVNEAFGFD
                     PSAEQANVART"
BASE COUNT          834 a         1491 c         1445 g          738 t
ORIGIN      
        1 cgggtcacgg ggtccgggcg cgtcaccgcc gctctgtttc cgggcggcgg tgacgcgcgg
       61 caccgagacc aagcaaggat tccgcgcgcg accgcatcgt cgtcgcatcg ggtgatgcac
      121 gcgtatccgc cccctgcaac gccagctcca tgatcagtgc cttcgcggtg acgaccagcg
      181 gcatcgcgag cgcgatgccg gcgatgcccc acagccagcc gccaaacagg atcgccagga
      241 tcaccgccag cggcgagagc tgcagcgagc gactgagaat gagcggcgtg acgaagtagc
      301 tctcggccga cgtgagcacg acgaatgcgc ccgcggtcaa gaggccgtag ccgagtgatt
      361 cgtgcgagac cgcgcccaca aagaacagca ccagcatgca gacgaaagcc ccgacgtgag
      421 gcacgtagtt ggccgtcgca gccagcacgc cccacagcag gggactggga atgcccaagg
      481 cccacatcac caggcccgtc gccactccca ggccggcatt gatcgtcgag atggtgagga
      541 ggtagcggga gatgccgttt tcgacgtcga gcaccgcctg gacgaccgtg cgtttttggg
      601 cgaaggacgt gcagagcgtg accgcatgcc gcagcagcga ctcgctgaac gccagcagga
      661 agaagcccag cacaaacacg atcaccgcca tcgccgtgac tttgccgctg actcccagca
      721 gtgcataggc caggtcggac gactgcactt ccacctgcac gggcggttcg gccccgtcgg
      781 accgcgtcag gccctgcact gcgtccgacg tttgctggag atcctggagc ggcccggccc
      841 gcttgggcaa caggttacgc acgcgctcga cggtttgcgg cgcttgcgcg agccagtgct
      901 gggcctggcc ggcgagcatc cagacaccca ccacgaagac cgccgccagg cagcccacga
      961 gcaagagcgc cgcggcgccg tccggaaagt tcagcctccg cagtcgccgc agaatcggcc
     1021 gcaagagcag cgccagcaat gccgccagca ccaccgggat catcacgtcc tgcgcgaaat
     1081 acagacccgc aatcgccgcg gcccacgcca gcacgatcag cggccagctc gcccgcgggc
     1141 gcgaggctgg agcggtcggc gcgctaggcg gcgatggccc gctggcaatt ggctgtggct
     1201 gcgacatgac atcttcccgc ctcgagcatc caggctgatg cgaggagtat ccgcaagtcg
     1261 catgccagag cagcggcggc tatttcgcgc ccgccagctt cagtacggcg tcgaactcag
     1321 ccttccgcac aggctgcacg ctcagccgcg agccttttcg cagcagctcc atgtctttca
     1381 gcgccgcgac gccgcggagc gtgtccaagg gcagcggctg cttgaagatt tgctccaggc
     1441 ggagatcgac catgaaccag cgcggatttt ctggcgtgct ggcagggtcg tagtggtggt
     1501 cgtttttgtt ccaggctgag gcatcgggat acgactcgcg gacgacctgg gccgtgccga
     1561 cgatcgccgg cggatcggtg ctgctgtgat agaacagcac gcggtcgccg actttcattt
     1621 cgtcgcgcat gaaattgcgg gcctgatagt tccgcacgcc gtcccagcag gtggtctggt
     1681 ccttggcctt cgccaggtcc tgaatcgaga agctctcggg ctccgacttc accagccaga
     1741 atttggacga ttttgccatg tcgcgttcaa gggccgccgc cgggcggcga atacgttagc
     1801 ggtgcgccgt ggggacgagg agtggaacat gctcggtatc ggtctgtggg agttcgccct
     1861 cttggccttc ctcctcgtcg tggtgctcgt cattgtaacg gccatcgtgg tggcccgctc
     1921 cagcaacacc aggcgataga tctccaacgc atggcagcag gaccggggat aacagtgatt
     1981 ctcctgggcg gcgtcgtact ggctgtcgtc gccgtcgtcg taacagtggt gatcgtcgcc
     2041 cttgccggca gtcgctgcga tgatcgctaa ggcttcgtcg gtgcggtcat ctcgcccagc
     2101 caggccgccg cctcatccac gctggcaaac gtccgcatgt tcaggccgcg attcaccgcc
     2161 accgtttcgg cgaaccgcaa gttgcccggc cgctcgtgct ggtggacatc gacatacgcc
     2221 agccaccgga gcagcatgcg attgcgcatt ccgccgcgtt cgacgatctg gtagatctca
     2281 accagcggca gtccgggtcc ggtcaggtcc tcctccacca ggatcgccgt gcagccccgc
     2341 tggacgcagt gggtgtgcac ctcggccaga tacgcctgca ccgttggcgg cgtattttcg
     2401 cccgttaccc gaaagtgaag gtagccgggc tgctccgtaa tcgtgagctg gtgagccatc
     2461 cagcgcgcga gtatccagcg cgtcttggtg ggggtcaagc catccgcccg gcacgctttg
     2521 acagcacctc cgcccagcta ctaaactcaa gacggccttc cagttttttc catcctccct
     2581 cctgatacgg aacgctcctt catggcgatc ctcggccgct cgcggtgcgt cctcttggcc
     2641 ttgggcctgg tgatgtgggg cgcccttgcg gcccgcgctc aagacgccgg cctcgatccg
     2701 aatcgactgc cgggaatcgt cgtggacgac gcggctgcga agctcgaagg cacgtggtcg
     2761 aagtcggtcc acacgcggcc gttcgtcggc gcgggctaca tctatagcca aggtggcgcg
     2821 gggcagtcgg tgaaattccc cgtcgagatc aacgaggcgg ggccctacca ggtgctgatc
     2881 tcgtacacgc cgggcaccaa ccgcacgcag aaggcgaacg tgctcgtgcc gacggcggac
     2941 ggtccgaaga cttttgtcat cgaccagcag gcgcggccgg ccgggccgta ttgttttcag
     3001 ccgctgggcg aactgacgct ggagccgggc aagctggaga tcgtcgtctc ggccgaaggc
     3061 agcaccaaag gcgtcgtcgt tgccgatgcg gtgcagattc tcacgcccga cggcttcaag
     3121 acctacaagg ccgattttga gaagaacacg cccaagctgg ccgacgcgct gaaggtcgat
     3181 cccaagaagt cggctgccaa gccagtggca aaagcggtgg agaagaagga agagccgccg
     3241 caggagcagc cgccggtgtt cgtccgcaag gagccggcca aaccgcgggc gaagctgacg
     3301 agcgagcagc tcgacaagct gatgcagcaa tacgtcggcg gcatcgacaa tgcgattctc
     3361 gtcgatgacg aggcgttctt gcggcgcgtc acgctcgacc tgattggccg acagccgacg
     3421 atggccgaga tgaccagctt cctcaaagcc gcgaccagtg gtcgcggttc ggaacatcgg
     3481 gccgctgcag tcgaaaaact ccttgccgtg ccagagttcg gcgcgaactg ggccaactac
     3541 tggagcgacg tgatcagcta ccgcacgccc tcgcccgagc tgacgttcct caactacacg
     3601 ccgttcaaga tctggctcgg ggagcaattc aaccagaata agggctggga tgagacgacc
     3661 tatcaggtca tcacggcgat cggcaaagtc gccgacaatc cggccgcgac ctacatcggc
     3721 tttcacggcg gcgacaaatc gcggctggcg gccgagacga cccgcgtgtt cctttcgacg
     3781 cagattcagt gcgccgagtg ccacgaccac aagttcgtcg atatgccgca agagacgttt
     3841 caccacatcg cggcgttctt cgtgcgggcc agcgccaagc ttccctggaa cgattcgagc
     3901 cagatcgtcg tttccagcaa gcccagcggc gagcacaaga tggagggcaa gggcgcggac
     3961 atgaagccga tcgcgttctc cgagcggcag gtcgatttgg gcaagagcga cgtggcccgc
     4021 cgggcggagc tggccgattg gatcgtgggg ccggagaacc cctggtttgc caaggcgttt
     4081 gtgaaccgcg tctgggcccg catgatgggc cgcggcttct gcgagccggt cgatgagatc
     4141 ggcgagctgg gcgaccgcgt gctgccggaa gtgcacaccg cgctggccga ccacttcgtc
     4201 gccggcaagc acgacgtgaa ggatgtcttt cgcgtggtcg cgctcagcaa gagctaccag
     4261 cgcgaaattc gcgacgccga caaaggccag aacaaagcct tcgtcgccat tcccgccggc
     4321 cgcctccgcg gcgacgaagt tttcgattcg ctgcaagcgg ccatcgccct gcccaacgtc
     4381 acgcccaagc aaggcgaagc caccaccgcc ttccgtttcc cgccgccccc caagagcact
     4441 cgcgacctcg tgaacgaagc cttcggcttc gacccctcgg ccgagcaagc caacgtcgcc
     4501 cgcacgat
//
LOCUS       DASRJB010000874         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_549160, whole genome shotgun sequence.
ACCESSION   DASRJB010000874 DASRJB010000000
VERSION     DASRJB010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549160"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3305)
                     /locus_tag="VFB80_22950"
     CDS             complement(<1..>3305)
                     /locus_tag="VFB80_22950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36714.1"
                     /translation="RTPEAWLARGRCRAELGRFEEALTDFNEAVELSPKDPDVLAARA
                     VFLADRGESAKATADFRAALALFDDAGPRPRYRFGVPIDLEIAQRDDVFDQMNAERPD
                     RRLLITRIVVRLQQGDVDGARSDVQRLSPLGWATPAAAANLSLGNRQAFERIRDETPR
                     NPRPEFNVMRLGLAPTAEPQTAELLEEAERMWQEHPNSQWHRRWLGLAQLRAGQYSEA
                     AANLEASLAGDNWQSHGITWPLLAMAHHHLGNKEATRRWLDRTAKWQELRGDAESRRV
                     GAATGSADILPEDLLYAMVFDREARTLIDGPDAARAERERPPSLAPDRTKATLAKVEA
                     AWNRAVELAGDNPLPWIQRGRWYALRGEREKADADFTKAAPLTRGELNKFLQAGWWVV
                     GPYPPNIAEFCPPQVDPDPAKPVHVIDPRTGLSAKPAEWRSVPTGSEGQIELAVPTQQ
                     NQPPAAYAMAYVYSPTDETRLLRLSRTHDLRVWVNSLLVLDLKQADFPTQPFYEDLHR
                     LPIALQSGRNVILIKTAAPTITARIADTPLDGVTLLAEQRRFHAACELLRERSRAGQP
                     IPPAALNNRLAHVMALDPQPELYLEYCKHLITRRNLGGGNCALIPNPVLDQHHAALLS
                     NAESLFAAKGLSFQAFDAALIAYRTGKIEQAKAFMAQVTGAKTFGVLSLRALIAHQSG
                     DASAAERLLAESIRDGQEQLAHLRGNHRSNPISPISAWWYEWASYLTLLREAEQTIRG
                     RTTESQALIVQSEELVTSQWSGDQATAAFDQPLTYQLIASQDQTKSPYYFLARGRRLA
                     ELGRFAEAEADFSKAVGRVPADTSPLVARAQFYAQRGDVERAAKDFGAALQQALPPPN
                     ENYVPGLVVGRELARHDAVFAKVAEQRPNDYRIWLGRSMHHADQGRWPEAAAENAKAD
                     WPRGFFILRTMERACLQHLAGDLQGYRETCQGLIAEESSAEEQLVFQVRRGILVACGI
                     APEGNRLAPNLVERAEQLAAAEADPWVHVGTALALYRAGRFDEARLRCEKALAPSAWL
                     DQRLALDCLRAMCLHQLGRPADARALLQAVQTCIAPPDSEPSIVLSKNGGPEQTIRLV
                     RQVLHAEATKLVS"
BASE COUNT          505 a         1097 c         1121 g          582 t
ORIGIN      
        1 cgctgactag tttcgtggcc tcggcgtgca ggacctgccg gaccagccgg atggtctgct
       61 cggggccgcc gtttttggac aggacgatgc tcggctctga atccgggggc gcgatgcagg
      121 tctgaacggc ctggagcagg gcccgggcat ctgcgggccg gccgagctga tggaggcaca
      181 tggcccgcag gcaatcgagg gccagtcgct ggtcgagcca ggccgatggc gcaagcgcct
      241 tttcgcagcg caggcgggcc tcgtcgaaac gcccagcccg atacagcgcc agcgccgtgc
      301 cgacgtgcac ccacggatcg gcttccgcgg ccgccagctg ctccgcgcgc tccaccaggt
      361 tcggcgcgag ccggtttcct tcgggagcga tgccacaggc cacgagaatg ccgcggcgta
      421 cctggaagac cagctgctct tcggcggatg attcttcggc gatcagtccc tggcacgttt
      481 cgcgatagcc ttgcagatcg ccggccaggt gctgcaaaca ggcccgctcc atggtgcgga
      541 ggatgaagaa tccacgcggc cagtctgctt tggcgttctc cgctgctgcc tcgggccagc
      601 ggccctggtc ggcgtgatgc atgctgcggc cgagccaaat ccggtaatcg ttcggccgtt
      661 gttcggcgac cttggcgaac actgcgtcgt ggcgggccag ttcgcggccg accacgaggc
      721 ccggtacgta gttctcgttc ggcggcggca aggcctgctg gagcgccgcg ccgaagtctt
      781 tcgccgcccg ctcgacgtcg ccccgctgag cgtagaattg ggcccgcgcc accagcggac
      841 tggtgtcagc cggcacccta ccgaccgcct tgctgaaatc ggcctcggcc tcggcgaaac
      901 gtcccagctc ggccagccgc cgaccacggg ccaggaagta gtagggggat ttcgtctggt
      961 cctgcgaagc aatgagctga tacgtgagcg gctgatcgaa cgcggcggtt gcctggtcgc
     1021 cgctccattg actggtgacc agttcctcgc tctggacgat cagcgcctgg ctctccgtcg
     1081 ttcggccgcg gatcgtctgc tcggcttcgc ggagcagcgt caggtagctg gcccactcgt
     1141 accaccaggc actgatgggg ctgatcgggt tggaccgatg gttgccgcgc aggtgcgcca
     1201 gttgttcctg gccgtcccga atgctctcgg ccagcaaacg ctccgcagct gaggcgtctc
     1261 ctgattgatg cgcgataagg gctcggaggg agagcactcc aaaagtcttc gcgccggtga
     1321 cttgggccat gaacgccttg gcctgctcga tcttcccagt ccggtaggcg atgagcgctg
     1381 cgtcgaacgc ctggaagctg agccctttgg cggcgaacag gctttctgca ttgctaagca
     1441 gcgcagcatg gtgctggtcg agcacgggat tgggaatcag cgcacaattg ccgccgccca
     1501 ggtttctgcg cgtgatcagg tgcttgcaat actccaaata cagctcgggc tggggatcca
     1561 gcgccataac gtgagccagg cggttgttga gcgccgccgg cggaatcggc tggcccgccc
     1621 gggagcgctc ccgcagtagc tcgcaggccg catgaaaccg ccgctgctcg gccagcaagg
     1681 tcacgccatc cagcggcgta tcggcgatcc gggccgtgat ggtcggcgcg gcagtcttga
     1741 tcaagatcac gttgcgtcca gactgcagcg cgatcggcag ccgatgcagg tcttcataga
     1801 agggctgtgt cggaaaatcg gcctgcttca gatccagaac gagtagtgag ttcacccaga
     1861 cccgcaggtc gtgcgtgcgg ctgagcctca gcagccgggt ctcgtccgtc ggcgaataga
     1921 cgtaggccat ggcgtacgcc gccggcggct gattttgctg ggtaggtacg gccagttcga
     1981 tctgcccctc gctgcccgtc gggacggagc gccactcagc cggtttggcg gacaggcccg
     2041 tccgcggatc gatcacgtgc accggtttgg ccggatcggg atcgacctgg ggcggacaga
     2101 actcggcgat attgggcgga tagggaccga caacccacca gccggcttgc aggaacttgt
     2161 tgagctcgcc gcgggtcagt ggggcagcct tcgtgaaatc ggcgtcggcc ttctctcgct
     2221 caccgcgcag ggcgtaccag cggccgcgct gaatccaggg cagcggattg tcgccggcca
     2281 gctccacggc gcggttccag gccgcctcga ccttggccaa cgtggccttg gtccggtctg
     2341 gggccagcga cgggggccgc tcgcgctcgg cgcgggccgc gtccgggccg tcgatcaaag
     2401 tcctggcctc gcggtcgaag accatcgcat acagcaagtc ctcgggcagg atgtccgcgc
     2461 tgccggtggc tgcgccgacg cgccgggact cggcgtcacc tctcaattcc tgccacttcg
     2521 ccgtccgatc gagccagcgg cgggtagctt ccttgtttcc caggtggtgg tgggccatgg
     2581 cgagcagcgg ccacgtgatg ccatgactct gccaattgtc gccagcgagc gacgcctcca
     2641 aattcgcagc ggcctcgctg tactgtccgg ctcgcaactg ggccagcccc agccagcgcc
     2701 gatgccattg cgagtttgga tgctcctgcc acatccgttc cgcttcctcg agcaattcag
     2761 ccgtctgcgg ctccgcggtc ggggccaggc cgaggcgcat gacgttgaat tcgggtcttg
     2821 gattccgggg agtttcgtcg cgaatgcgct cgaacgcctg gcgatttccc aggctgagat
     2881 tcgcggccgc cgccggcgta gcccagccaa gcggcgacag acgctgcacg tccgaccgcg
     2941 cgccgtccac gtccccttgt tgcaagcgaa cgacgatccg cgtgatcagc agccgtcgat
     3001 ccggccgctc cgcgttcatt tgatcgaata cgtcatcccg ctgggcaatt tccagatcga
     3061 tcggcacgcc gaagcggtag cgcggcctgg ggccggcgtc gtcgaacagg gcgagcgccg
     3121 cacgaaagtc ggcggtggcc ttggccgact cgccgcggtc ggcgaggaag accgcccgcg
     3181 ccgccagcac gtcgggatcc ttcgggctca gttcgacggc ctcgttgaaa tcggtcaggg
     3241 cctcttcgaa ccgccccagc tcggcccggc agcgtccacg ggccagccag gcttcgggcg
     3301 tccgt
//
LOCUS       DASRJB010000875         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1950447, whole genome shotgun sequence.
ACCESSION   DASRJB010000875 DASRJB010000000
VERSION     DASRJB010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950447"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..516)
                     /locus_tag="VFB80_22955"
     CDS             complement(97..516)
                     /locus_tag="VFB80_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36715.1"
                     /translation="MSNSFRLPRPGGPALLAALALVAGCSQPAAVPAVVAGKVTLGGV
                     PLTAGGVLFMNDAGDAASAELAADGTYTLQCKPARYKVAVTPPAPVDPLGTPAGAPPR
                     PAAPPPAVPKRYHDLATSGLTCDVKEGNNTFDIPLTR"
     gene            complement(582..1580)
                     /locus_tag="VFB80_22960"
     CDS             complement(582..1580)
                     /locus_tag="VFB80_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36716.1"
                     /translation="MGKTRTLRAGFTLVELLVVIAIIGVLVALLLPAVQAARESARRM
                     KCGNNLKQIGLALHNYHDRMGSFPMGTFGVFMHSWQLAILPEVEQANMLDKVVFSVVA
                     LRGSGAPEGPNRTLLNGWTAAYNWCPSSNTPRLCLRVGPGGEKFSTSSYNGISGASVS
                     ATDASDPVGGGRCVSGNQGFACANGALVPNRSVRIAEITDGTSNQIIVGECSAWGRNA
                     AGAQVDIRGSGEWGCWIGCGAAAGPPEPGGPFPWSAPWCRNVATIRYPINMRTEVGGA
                     GGNHRDGTNNALHSHHPGSVMVLRADGSVHSQSQTTEYLLLRNLSIRDDGAPTTET"
     gene            complement(1726..2139)
                     /locus_tag="VFB80_22965"
     CDS             complement(1726..2139)
                     /locus_tag="VFB80_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36717.1"
                     /translation="MNESSTSPPPAARPADRVPPNQAMILLRGGAGAIFGAYLGYLAF
                     WWLASQGFYGMMIIGAAVGLLSGLAARGKSTTLGILCVIIALAASIFAEWKVFPFIKD
                     KSLQFFLTHLHQVMPVHLLMIGLGAAAAYWFGQGR"
     gene            complement(2145..3020)
                     /locus_tag="VFB80_22970"
     CDS             complement(2145..3020)
                     /locus_tag="VFB80_22970"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN36718.1"
                     /translation="MTRLLALVLLLIATTLSAQEKAASDAPPGVKYEKGIVSGKGGDT
                     ELHLDIAYPENANKPLPCIVVIHGGGWRGGNYTVHDSQILEIAKAGFVSATIQYRLVP
                     SARWPAQIEDVKCGVRYLRANAEKYHIDPERLGAVGFSAGAHLSMLLGTMDKQDGLEG
                     SGGNAGQSSKVQAVVAFFGPTDLAAKDFPANVNGMIYDFLGGLPDEKPELFKAASPVS
                     YVDKTDAPTLIYHGTKDALVPYNQAFLMTDKLTAAGVPGRVEMLLGANHGWQGAELLR
                     TFEGTMTFFKQQLKK"
     gene            complement(3050..4291)
                     /gene="cbiE"
                     /locus_tag="VFB80_22975"
     CDS             complement(3050..4291)
                     /gene="cbiE"
                     /locus_tag="VFB80_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZN36719.1"
                     /translation="MAANKVQIIGIGDDGLEGLTAAARQLVAQAELLIGARQTLAAAA
                     TSKAEKVEIGGDLDAVVRKLESAAGKRTVVLTTGDPLFYGTARYLVGRLGKDRFEVTP
                     HVSSMQLAFARVKESWDDAYLTNLATQPLELVVEKARTAAKVGVFTTEAAPPDAVAQA
                     LLDRKIDYFTAYVCENLGSPDERVTHGELADIARQEFSPLNVLILVRKPNVPDRPAAM
                     IGRKLFGNPDESFMQSRPKRGLLTPAEVRAVALSLLDVGPTSTIWDVGAGSGSVAIEA
                     AQIASGGQVFAIEMDPEDYALTASNAERFGVRNLTPVLGKAPEAWAGLPPPDAVFVGG
                     TGRQVRRIVELALAPLRSGGRIVANVGSIENLTAVREVLLAGAGDASVLMINLARGND
                     QLEWLHFEALHPTFLVSAVKK"
     gene            4493..>4794
                     /locus_tag="VFB80_22980"
     CDS             4493..>4794
                     /locus_tag="VFB80_22980"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36720.1"
                     /translation="MPRVLVTPTLLRNVPGLYCDTLLQGGFEVVYPPEGCDTMQRENL
                     LPLLATADAMLASTERLSREVLAGSKLRAVARMGVGYDSVDVPAATDLGIAVTITPG"
BASE COUNT          791 a         1536 c         1622 g          845 t
ORIGIN      
        1 ttcccatggg ccacgcaccg agatcacgga gaagtgaagt ggactgcccc ctctgtgagc
       61 tccgtgcctc tgtgtgagac tcctgttgga tacgttctac cgcgtcaggg gaatgtcgaa
      121 cgtgttgttg ccttccttga cgtcgcaggt cagcccgctg gtggcgaggt cgtggtagcg
      181 cttggggacg gccggaggag gcgcagccgg gcggggtggc gcgccggcgg gagtaccgag
      241 cggatcgacc ggggcgggcg gcgtcaccgc gactttgtaa cgcgcgggct tgcactgcag
      301 cgtgtaggtg ccgtcggctg ccagctcggc cgaggcggcg tcgcccgcgt cgttcatgaa
      361 cagcacgccc cccgcggtaa gcggcacgcc gcccagcgtc accttgcccg cgacgacggc
      421 tggaactgcg gccggctgcg agcagccggc gacgagcgcc agggccgcga ggagcgccgg
      481 gccgccgggg cggggaagcc gaaacgagtt gctcatgttc gcacctcata gtagccatct
      541 cgctccgcga gatgttcgcc tcacgcggag cgtgaggtca actatgtctc ggtggtcggc
      601 gcgccgtcgt cgcggatcga gaggtttctt agcaggaggt actcggtcgt ctgcgactgc
      661 gaatgcacgc tgccatcggc gcggaggacc atgacgcttc ccgggtgatg ggaatgcaag
      721 gcgttgttcg ttccgtcgcg gtgattgccg ccggcgccgc ccacctcggt ccgcatgttg
      781 atcggataac ggatcgtcgc gacgttccgg caccaggggg ccgaccaggg aaatgggccg
      841 ccgggctcgg gcgggccagc agcagcgccg cagccgatcc agcagcccca ttcgccgctg
      901 ccgcgaatgt cgacttgcgc gccggctgcg ttccggcccc aggcagagca ttcaccgacg
      961 atgatctggt tgctggttcc atcggtgatc tccgcgatcc gcacgctgcg gttggggacg
     1021 agcgccccgt tggcgcaggc gaagccttga ttgccggaga cgcagcggcc gccgcccact
     1081 gggtcgctgg catcggtggc gctgacggac gcgccggaaa tgccgttgta cgaggaggtc
     1141 gagaacttct cgccgcccgg cccgacgcgc aggcacagcc gcggggtatt gctcgacggg
     1201 caccagttgt aagccgccgt ccagccgttg agcagcgtgc gattggggcc ttcgggagcg
     1261 ccggagccgc gcagcgcgac gacgctgaag accaccttgt ccagcatgtt ggcctgctcc
     1321 acctcgggca ggatcgccaa ttgccaggag tgcatgaaga ccccgaacgt gcccatggga
     1381 aagctgccca tccgatcgtg atagttgtgc agcgccaggc cgatctgctt caggttgttg
     1441 ccgcatttca tgcggcgggc cgattcccgg gccgcttgca ccgcgggcag gagtagtgcg
     1501 accagcacgc cgatgatcgc aatcacgacc agcagctcga ccagcgtaaa gccggcccga
     1561 agagtgcgag tctttcccat ggtcccttcc tccaagactg caggaataga aaccaaaacc
     1621 gccgctcgct agccgccaga ttccgggcgg catcagcgcg cgattgtagg cctggcggtg
     1681 ctctcaagca acgaaatgcc gcggtcggta gataacgacg cccaactagc gcccctgccc
     1741 aaaccaatag gcggccgcgg cgcccaggcc gatcatcagc aggtgcacgg gcatgacctg
     1801 gtgcaggtgg gtcagaaaga actggaggct cttgtccttg atgaagggaa agaccttcca
     1861 ttcggcgaag atggaagcgg ccagcgcgat gataacgcag aggatgccga gggtggtgct
     1921 cttaccgcgg gcagccaggc cggacagcag gccgactgcg gccccgatga tcatcatgcc
     1981 gtaaaagccc tggctggcca gccaccagaa agccaggtag ccgaggtacg cgccgaagat
     2041 cgctcccgcc ccgccgcgca gcaggatcat cgcctggttg ggcggcacgc ggtcggcggg
     2101 cctggccgcc ggcggagggc tggtcgagga ttcgttcatc gtccctattt cttcaattgc
     2161 tgcttgaaga acgtcatggt gccctcaaaa gtccgcagca gttcggcgcc ttgccagccg
     2221 tgattagcgc cgaggagcat ttcgactcgc ccgggaacgc cggcggccgt cagcttgtcg
     2281 gtcatcagga aggcctggtt gtaaggcacc agggcgtcct tcgtgccgtg atagatgagt
     2341 gtcggggcgt cggtcttatc gacgtaactg accggcgagg cggccttgaa gagctcgggc
     2401 ttctcgtcgg gcagcccgcc gaggaagtca tagatcatgc cgttgacgtt cgcgggaaag
     2461 tccttcgccg cgaggtcggt cggcccgaag aaggccacga cggcctgcac cttgctcgat
     2521 tgccccgcgt tgccgccgct cccttccagg ccgtcctgct tgtccattgt gcccagcagc
     2581 atcgacaggt gggctcccgc cgagaagccg accgcgccca gccgctccgg gtcgatatgg
     2641 tatttctcgg cattggcccg caggtagcgg acgccgcact tgacgtcctc gatttgcgcc
     2701 ggccagcggg ccgacggcac gaggcgatac tgaatcgtcg ccgagacgaa gccggccttg
     2761 gcaatttcga ggatctggct gtcgtgcacc gtgtaattgc cgccccgcca gccgccgccg
     2821 tggatcacga cgatgcaggg cagcggcttg ttggcgtttt ccgggtatgc gatatcgaga
     2881 tgcagctccg tatcaccgcc cttccctgag acgatgcctt tttcgtattt cacgcccggc
     2941 ggcgcatcgg aggcggcttt ctcctgggcg gagagcgttg tggcaatgag cagcaggacg
     3001 agagcgagga gtcgggtcat ggtagtgctt gttgaagtga tctagcgatt catttcttca
     3061 cggcggacac caggaacgtc ggatgcaggg cttcgaagtg cagccattcc agttgatcgt
     3121 tgccgcgggc caggttgatc atcagcacgc tggcatcccc cgcgcctgcg agcaatacct
     3181 cgcggaccgc ggtgaggttt tcgatgctgc cgacattggc cacgatccgg ccgccgctgc
     3241 ggagcggcgc gagcgccagc tcgacgattc gccgcacctg ccggcccgtg ccgccgacga
     3301 agaccgcgtc gggcggcggc aggccggccc aggcttccgg ggctttgccc agcaccggcg
     3361 tcaggttccg cacgccgaac cgctcggcat tgctggcggt cagggcgtag tcctccgggt
     3421 ccatctcgat ggcgaagacc tgcccgccgc tggcgatctg ggccgcctcg atggcgaccg
     3481 acccgctccc cgcgccgacg tcccagatcg tgctcgtcgg gccgacgtcg agcagcgaca
     3541 gggccacggc ccggacttcc gccggcgtca gcagcccgcg cttgggccgc gactgcatga
     3601 acgattcgtc cggattgcca aacagcttgc ggccgatcat tgccgccggg cggtcgggca
     3661 cattcggctt gcggacgagg atcagcacgt tgagcggcga gaactcctgc cgtgcgatat
     3721 cggccaattc gccgtgcgtc acgcgttcgt cgggcgagcc gaggttctcg cagacgtagg
     3781 ccgtgaaata gtcgatcttg cggtccaaga gggcctgggc cacggcgtcc ggcggcgcgg
     3841 cttcggtcgt gaacacgccg accttcgccg cggtacgggc cttttcgacg accagctcca
     3901 gcggctgcgt cgccaggttc gtcaggtagg cgtcgtccca gctctccttc acgcgggcga
     3961 aggcgagctg catgctgctg acgtgcggcg tgacttcgaa gcggtccttg cccaggcggc
     4021 ccacgaggta gcgggccgtg ccgtagaaca gcggatcgcc cgtggtgagg acgacggtcc
     4081 gcttgcccgc cgccgactcc agcttgcgga cgacggcatc gaggtcgccg ccgatttcga
     4141 ctttttcggc tttactggtt gcagccgcgg ccagcgtctg gcgggccccg atcagcagct
     4201 ccgcctgggc gacgagctgg cgagccgcgg cggtcagccc ttcgaggccg tcgtcgccaa
     4261 tgccgatgat ttgaaccttg ttggctgcca agggcgcctc ccatgcaata gtggcaccat
     4321 tgtatggcaa aaatcgaaga accaaagaac caaagaacca aagtaggcca agagacagaa
     4381 atcgatgact agggtttagt atttcggttt ttggttcttt ggttcttcgg ttcttggaaa
     4441 ttcgcgtcct cgcggataca accagatttc cccccagtcg gaacaacatc gcatgcctcg
     4501 cgtcctcgtt acaccgacgc tccttcgcaa cgttcccggc ctttattgcg acacgttgct
     4561 tcaggggggc ttcgaggtcg tttatccgcc ggaaggctgc gacacgatgc agcgcgagaa
     4621 cctgttgccg ctcttggcca cggccgatgc aatgctcgcc agcaccgagc ggctgtcgcg
     4681 cgaggtgctg gccggcagca agctgcgggc ggtcgcacgg atgggcgtgg gctacgactc
     4741 agtcgatgtg ccggcggcga ccgatctggg catcgccgtg acgatcacgc cggg
//
LOCUS       DASRJB010000876         5957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2228575, whole genome shotgun sequence.
ACCESSION   DASRJB010000876 DASRJB010000000
VERSION     DASRJB010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228575"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..604
                     /locus_tag="VFB80_22985"
     CDS             242..604
                     /locus_tag="VFB80_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36721.1"
                     /translation="MRFSIRDLLLLTLIVGLVTGWALDRWRLAKQRADQDHRVRRLVE
                     AQLARAAELENRLVQQIQAYQQANAEFKEVVDNVKYAAENEELVKHGYLDTLRGLRLG
                     RPTSRMPQLTTEPAESDH"
     gene            607..882
                     /locus_tag="VFB80_22990"
     CDS             607..882
                     /locus_tag="VFB80_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36722.1"
                     /translation="MRFSIRDILLVTVIVALATGWALDRWRLARHAAELELRSRQLMV
                     EAELSRAQALLERDVAAVNLERVELLEQAKKFYAEAGAADAAAEADK"
     gene            899..2125
                     /locus_tag="VFB80_22995"
     CDS             899..2125
                     /locus_tag="VFB80_22995"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HZN36723.1"
                     /translation="MDRRLFLASSMSTGMMAAATAAQTQPAAAAEVDVSGLSKANIPT
                     PALIVDLAALEANIQKMADHCRQAKVGFRPHAKTHKCPDIAKRQIAAGASGICAATVP
                     ELEALAAAGIPGLLLTSPIVDPAKIARVVAIASRGGQVMLSVGSPRQAELLAEAADAA
                     KVTLDVLVDLDVGDRRTGSLPLEPAVELARLIAKSPRLKVRGVQAYAGHASHTVGFEA
                     RQKVSQTAIGKAVETKQRLQKAGFDAKILSGGSTGTYNIDSAIEGMSELQVGSYVFMD
                     LDYRRIGGQGGSERYADFQHSLTVLATVVNATHPDRVTVDAGTKALDTTTVQIPEAKG
                     RPGLTYARAGDEFGALSLGEGGKLPALGERLEFIVPHCDPNVNLYDRIYACRGERVEA
                     IWPVAARREFAVAGLR"
     gene            2131..3123
                     /locus_tag="VFB80_23000"
     CDS             2131..3123
                     /locus_tag="VFB80_23000"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN36724.1"
                     /translation="MADLADARAVLARYFPSAEFVQRLEPLGNAGGWSGSQLWRVGTP
                     AGEFCLRRWPRQHPQPQQLVLIHTVMARVSGDLPAVPLPQATTSGATFVEQDGQLWEL
                     VTWRPGKADYRERPSRERLQAAMQTLARFHTLAGRGQSVEPTVPPALCDRAERWAELA
                     ASGVAAIERSLARPLGSEIDQLAPRLLMLACTAHNDPERNPGRWMHPQLPLQPAIRDI
                     HRDHVLFTGDEVTGLIDFGAMRIDTPLADVARLVGSLAGDDPEAREFALNAYSELRPL
                     NDDDRGFIDLLDETGLILSAFNWLAWLYVERREMGPTAPIVRRLNEIMLRLAVR"
     gene            complement(3554..4393)
                     /locus_tag="VFB80_23005"
     CDS             complement(3554..4393)
                     /locus_tag="VFB80_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36725.1"
                     /translation="MSTLWLPIAATFLLGAEPPQPASVVAAPPLVTEAPAEEPPDAFT
                     PSPEFQDWITRLVREQLPNPYEKKKNWGHQVKTFDGVSVQLKDGQLKTHRKFKQANDG
                     TWQYYKVTLKNPEEKFEIRVAKIEQLPNNKVGMDVSAVAQLEVYGRQSLWDHGIQIFS
                     LSAEADARVRLSAHCEIATRLDPTRFPPDVYLDPEVKAAKFDIPEFKLRRVGRADGPI
                     VRSLSNAAREALEDKLREDNAKLVASLNRQIAKQEKKLKLSLADVMSSKFGKMFSQSD
                     DSK"
     gene            complement(4532..5707)
                     /locus_tag="VFB80_23010"
     CDS             complement(4532..5707)
                     /locus_tag="VFB80_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN36726.1"
                     /translation="MPNANFDSRAQDLLKSLADARQLKRFQRLSGPMGATANIEGRGE
                     VVVLCSNNYLGLADHPEVVQAGIEGLKRYGAGTASVRFICGTLDCHARIEETIARFVG
                     TQSALTYVSCWNANEAVFPTLVGPDDVILSDELNHASIIDSIRLVSKAIPREVYKHSD
                     LADLAAKLKQYPGKACRWVVTDGVFSMEGDVAKLPELAAICREHSAMLVVDDSHGVGV
                     LGAGGRGTPEHFGLLGQVDMLTGTLGKALGGAAGGYVAAAPHAIEMLQQRGRPSLFSN
                     ALPATVACSARRAIEIVEREPQRVARLHANVRRVREGLTKLGFQLHESPTAIIPIMIG
                     DEAETIAKSNRLLELGVLVIGFGYPVVPKGQARLRVQVSAALEESHLQRALDAFAKL"
BASE COUNT         1028 a         1913 c         1965 g         1051 t
ORIGIN      
        1 aggccaggac taccagggcg accaacagga gcgatttcat ggtgttcctc caggtggcta
       61 aacttgaaat cggccggttt gatcgcaagc cttgtgccaa ggccgattat gccggagagg
      121 cacggcctag aggcaattcg ccagcgtccg tgctggccgg cgagcaaaca tagtggttcg
      181 gccgtgagca gcactgcggc gttggtagac gtcgcgggag cccgaacatt acaatccggc
      241 catgcggttt tcgatccgcg acctcctgct gctgaccctc atcgtcggtc tcgtcaccgg
      301 ctgggcgctg gaccgctggc ggctggccaa gcagcgagcg gaccaggatc accgagtccg
      361 ccgactggtt gaagctcagc tggcgcgcgc cgcggagtta gaaaatcgat tggtccagca
      421 aatccaagct tatcagcagg ccaatgcaga gttcaaagag gtagtggata acgtcaagta
      481 tgcggcagag aatgaggagc tagtcaagca cggatacctc gacacgctcc gcggcttgcg
      541 gctgggtcgg cctaccagcc ggatgccgca gctgaccact gagccggcag agagtgacca
      601 ctgagcatgc gattttccat ccgcgacatc ctgctagtca ccgtcatcgt cgccctcgcc
      661 accggctggg cgctcgaccg ctggcggttg gcgaggcacg cggccgagct ggagctgcgc
      721 agccggcagc tgatggtcga ggcagagctt tcgcgggccc aggcgctcct ggaacgcgat
      781 gttgcggccg tgaatcttga gcgagttgaa ttgttggagc aggccaaaaa attttacgca
      841 gaagcgggcg ccgccgacgc ggctgcggaa gccgacaagt gatgccaagg aaccgaatat
      901 ggaccgccga ctttttttag cctcgagcat gagcaccgga atgatggcag ccgcgaccgc
      961 cgcgcagacc cagccggccg ccgccgccga agtggatgtg tccggcctgt cgaaggccaa
     1021 catccccacg ccggcgctga tcgtcgatct ggcggcgctg gaagccaaca tccagaagat
     1081 ggcggaccac tgccggcagg cgaaggtcgg cttccggccg cacgccaaga cccacaagtg
     1141 ccccgacatc gccaagcggc agatcgccgc cggcgcgagc ggcatctgcg ccgcgacggt
     1201 gccggagctc gaggccctcg ccgccgcggg cattccgggg ctcctgctga cctcgccaat
     1261 cgtcgatccg gcgaagatcg cccgcgtcgt cgcgatcgcc agccgcggcg ggcaggtcat
     1321 gctctccgtc ggcagcccgc ggcaggctga gctgctcgcg gaagcagcgg acgcggcgaa
     1381 ggttacgctg gatgtgctcg tcgatctcga cgtcggcgac cggcggaccg gctcgctgcc
     1441 gctcgagccg gcggtcgagc tggcccggct gatcgccaag agcccgcggc tgaaggtccg
     1501 cggcgtgcag gcctacgccg gccacgcctc gcacacggtc ggcttcgaag cccggcagaa
     1561 ggtctcgcaa accgcgatcg gcaaggccgt ggagacgaag caacggcttc agaaggcagg
     1621 cttcgacgcc aagattctct cgggcggcag cacgggcacc tacaacatcg actcggcgat
     1681 cgaaggcatg agcgagctgc aagtcgggtc gtacgtcttt atggacctcg actatcgccg
     1741 catcggcggc cagggcggat cggagcgcta cgccgacttc cagcacagcc tcaccgtgct
     1801 ggcgacggtg gtcaacgcca cgcatcccga ccgcgtcacg gtcgatgcgg ggacgaaggc
     1861 cctcgacacg acgacggtgc aaattcctga ggcgaagggc cgacccggct tgacctatgc
     1921 ccgagccgga gacgaattcg gcgctctctc gctcggcgaa ggcggcaagt tgccggccct
     1981 cggcgagcgg ctggagttca tcgttccgca ctgcgatccg aacgtgaacc tctacgatcg
     2041 gatctatgcc tgccgcggcg agcgggtcga agcgatctgg ccggttgccg cgcggcgcga
     2101 gttcgccgtg gccggactac gataaagagc atggccgatc ttgcggacgc gcgggccgtc
     2161 ctggcccgct atttccccag cgccgagttc gtccagcgcc ttgagccgct gggaaacgcc
     2221 ggcggctgga gcggctcgca gctgtggcgc gtcggtacgc ccgcaggcga attctgcctc
     2281 cgccgctggc cacggcagca tccacagccg cagcagttgg tgctcattca cacggtgatg
     2341 gcccgcgtta gcggcgattt gcccgccgtc ccgctgcctc aggcgacaac gagcggtgcc
     2401 acgttcgtcg agcaggatgg tcagctgtgg gagctggtga cctggcggcc gggaaaggcc
     2461 gactatcgcg agcgtccgag ccgcgagcgg ctgcaggcgg cgatgcaaac cctcgcccgc
     2521 tttcacacgc tggccggtcg gggccagagc gtagagccga ccgtgccgcc cgcgctctgc
     2581 gaccgagcgg agcggtgggc tgagcttgct gccagcgggg ttgcggctat cgagcgatcg
     2641 ctggcgcgtc ccctgggtag cgagatcgac caactagcgc cgcgactgct aatgctggct
     2701 tgcaccgctc acaacgatcc cgagcggaat ccaggccgct ggatgcatcc gcagcttccg
     2761 ctccagcccg caatccgcga catccaccgc gatcacgtgc tgttcaccgg cgacgaggtg
     2821 accgggttga tcgacttcgg agcgatgcgg atcgatacgc cgctggccga cgtcgcgcgg
     2881 ctcgtcggca gcctcgccgg cgatgacccc gaagcgcggg agtttgcgct caatgcgtat
     2941 tccgaactgc gtccgctgaa cgacgacgac cgggggttta tcgacctgct cgacgagacg
     3001 gggctgatcc tctccgcctt caactggctc gcgtggcttt atgtcgaacg ccgcgagatg
     3061 gggccgaccg cgccgatcgt ccgccggctg aacgagatca tgctgcgcct cgccgtcagg
     3121 tagtagtccg tctatcggct ttggttggta ggagccgatt tgagggccat actaaacatt
     3181 ggtacgctat ttgcagtata gttcccattg gcgcggcgga ctgccgtttt ggccgcccgg
     3241 cgcaaggtcg gccgttcatt ctcaatttgt cgatggcaac taagggcgga atcgtcgccg
     3301 ctccgctcgg cagcagaata taacgaaaac tccccaccag gctctctaag ccttctaacc
     3361 ctcaaatgcc aaaacgccgg agaaatgccg gaagtcctcg cgtgccaagc agttgcggca
     3421 cgatggcggc atgcgacgcg cgtgtgcgaa gcagtgctgt tggcagtttt cgttatattc
     3481 gtcgccggcc cttaagggca gccgacgggc cgcgtggtcc gcctcgatgc tcctacaggt
     3541 gcctgcagaa gtctcatttc gagtcgtcgg attgcgagaa catcttgccg aacttgctgc
     3601 tcatcacgtc ggccagcgag agcttgagct tcttctcctg cttggcgatc tgccggttga
     3661 ggctggcgac gagcttcgcg ttgtcctcgc ggagcttgtc ttcgagggcc tcgcgggcgg
     3721 cgttgctgag cgagcggacg atcgggccgt cggcgcggcc cactcggcgg agcttgaatt
     3781 cgggaatgtc gaacttggcg gccttcactt cgggatcgag atagacgtcg ggcggaaatc
     3841 gcgtcgggtc cagccgcgtg gcaatttcgc aatgggccga cagccgcact cgggcgtcgg
     3901 cttccgccga gagactgaag atctggatgc cgtggtccca cagcgactgc cgaccataga
     3961 cctccagttg ggccacggcc gagacgtcca tgccgacctt gttattggga agctgctcga
     4021 tcttggcgac gcgaatctcg aacttctcct cgggattctt gagcgtcacc ttgtagtact
     4081 gccaggtgcc gtcgttggcc tgcttgaact tgcggtgcgt cttgagttgg ccgtccttga
     4141 gctgcaccga gacgccgtcg aaagtcttga cctgatgccc ccagtttttc ttcttctcgt
     4201 aagggttcgg cagctgctcc cggacgagcc tggtgatcca gtcttgaaac tccggcgaag
     4261 gcgtgaacgc gtcaggcggc tcttcggccg gcgcttcagt caccagcggc ggcgcggcga
     4321 cgacgctggc cggctgcgga ggctcggcgc caagcagaaa cgtagcggca atgggcagcc
     4381 agagggtgga catgcaagca accttacccc ggagattagg gcctgcctcc ggttttacaa
     4441 agtccaaaga accagccgcg gatgccgcgg atgacacgga ttcgggcaag tgaaatccgt
     4501 gaaattccgt gtatgtccgc ggcttttccg cctacagctt cgcaaatgcg tcgagcgccc
     4561 gctggaggtg cgactcttcc agggcggcgg acacttggac gcgaagccga gcttgcccct
     4621 tggggacgac ggggtaaccg aagccgatga ccagcacgcc cagttcgagc aggcgattgc
     4681 tcttggcgat cgtctcggcc tcgtcgccga tcatgatcgg gatgatcgcc gtgggcgatt
     4741 cgtgcagctg gaagccgagt ttcgtcagcc cctcgcggac gcggcggacg ttggcgtgca
     4801 ggcgggccac gcgctgcggc tcgcgctcca cgatttcgat cgcccggcgg gcgctgcagg
     4861 cgaccgtggc gggcagcgcg ttggaaaaca ggctcggccg gccgcgctgc tggagcattt
     4921 cgatcgcgtg cggggccgcg gccacatagc cgccggcagc gccgccgagg gccttgccga
     4981 gcgtgcccgt gagcatgtcg acctggccca gcagtccaaa atgctccgga gtgccgcgac
     5041 cgcccgcgcc gagcacgccg acaccgtgcg agtcatcgac cacgagcatg gcgctgtgct
     5101 cgcggcagat cgcggcgagc tccggtagtt tcgcaacgtc gccttccatg ctgaatacgc
     5161 cgtcggtgac cacccagcga cacgccttgc cggggtattg cttcagcttc gcggccaggt
     5221 cggccagatc gctgtgcttg tagacttcgc gcgggatcgc tttgctgacc aggcggatgc
     5281 tgtcgatgat gctggcgtgg ttcagctcgt cggagaggat cacgtcgtcc ggcccgacaa
     5341 gcgtaggaaa gaccgcctcg ttggcgttcc agcaactcac ataggtcagc gccgactgcg
     5401 tgcctacgaa ccgcgcgatc gtctcctcga tccgcgcgtg gcaatcaagc gtgccgcaga
     5461 tgaaccgcac cgacgccgtg cccgccccgt accgcttcag cccttcgatt cccgcctgaa
     5521 cgacctccgg atgatcggcc agacccaggt agttgttgga gcagagcacg accacttcgc
     5581 cgcggccctc gatgttcgcc gtcgcgccca ttgggcccga caatcgctga aaccgcttca
     5641 gctgccgcgc atcggccagc gatttcagca ggtcctgggc acgggaatcg aagttggcgt
     5701 tgggcatgga ataagcctgc cgctgagggt ctcaccagaa ttcccttggg cacacattct
     5761 ttcggcgctc tgcttcgttt acggctatcg aagcggagct tcgagtcgtg cggtccccag
     5821 cggagcttgg gaccgagttg cctttgaacc cggaacttcg aggacaacct tgatcgcctc
     5881 gccggcttgc atcagcttca ggccgcgctc gaattccgcg agcggcagct ggtgcgtaac
     5941 gatctggtcg aggtgca
//
LOCUS       DASRJB010000877        10141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1112354, whole genome shotgun sequence.
ACCESSION   DASRJB010000877 DASRJB010000000
VERSION     DASRJB010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112354"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFB80_23015"
     CDS             <1..590
                     /locus_tag="VFB80_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36727.1"
                     /translation="ASHFVSTDADAANEQGDVSAEISAALLELTQTSRQRQVAEQRQT
                     DRMQELSSEPTAADWSAEPAVVVTRLGGTPRSVTLLLGSLLACTAAIVMFRASSAAVL
                     PPKIQTARELAAVLEIPVIGSAASLRTAAARLRQRLFKPVYVRLAGHLAEGVLALAAA
                     ACLISITVEPTLASQVLADPFGTLSEVMGRFVSSR"
     gene            763..2271
                     /locus_tag="VFB80_23020"
     CDS             763..2271
                     /locus_tag="VFB80_23020"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN36728.1"
                     /translation="MYESFFRLTHRPFVPAPLAEAYIPTSSLDHARQTLVRCLDRGEG
                     PGLIIGPAGTGKSLLCQVLAKHFRGRFQIALLSAARLCTRRALLQNILFELRLPYREL
                     DESELRLSLIDHLEPRAGGTEGLLLLVDEAHTLPLRLLEEIRLLTNLVQGGQSRVRLV
                     LAGGLALEERLNNPKLDSFLQRIVARCYLQPLGRDETIFYVQEQFRRCGAAADSVFTG
                     DAQTAIHTATDGIPRLINQLCDHALMLAALGGERTIDSGGVEEAWADLQQLPVPLHDP
                     PATRSTSSGGSTGGIVEFGQLGEAPNNQTVPLADAALANIDAIDQGLSLLKTSEAAAE
                     AAGESSGEFSPADPSGTEVELIFHSSHDPFGGHWEHEEVVIDRYASLEDAAVRNRRQV
                     TSDEGRAIGAAVSAAAVPGEPAQPREDEPVSQNSMTIPASAAFHPASDPLLPEEPPTP
                     QSRRATVSLNELGQDDRDLIVVEDDQAGAVPPLAGRPRRPEYRQLFSSLRNK"
     gene            2278..3213
                     /locus_tag="VFB80_23025"
     CDS             2278..3213
                     /locus_tag="VFB80_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36729.1"
                     /translation="MARMLQALKNLEARSKKPAAAGGLLGHLADNAGRTKPPAQPAAN
                     PAQPAPNPAPPAPNNTALVPVYLQAQDVAADSAAPVVAAPPAKPQPDPPPAIKRDRFG
                     INRPPANLERAVRRTLTDSSRAQPFQQLAHRLARDVEQTDSKTLLWISIGESRGGYES
                     LLHSAALLAEALEGPTLLIDADLGRRSLSGDLDYGQLTGLAELLKNDERPQPLCRPTA
                     FERLSLLPAGRASYVDLLAGPRLENLLQELAGEYSLLLIDGGRSSDAAAAALARVSDA
                     TYFVVQLGVVEGCAAQAALRDFRAAGAKVLGCVAT"
     gene            3326..4264
                     /locus_tag="VFB80_23030"
     CDS             3326..4264
                     /locus_tag="VFB80_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN36730.1"
                     /translation="MPTCLVTGGAGFVGSHIAEALVRRGDKVRVLDNFDTGKRENLQA
                     VLGQVELITGSVADAAAVAKAVAGCEFVFHQAALASVPRSIDDPLATHAACATGTLNV
                     LNEARKAKVRRVVYAASSSAYGNQPQPQKRETDLPSPLSPYAAAKLAAELYCEAFWHS
                     YGLEAVALRYFNVFGPRQDPSGPYAAVIPIFIQKILAGQQPTIFGDGLQTRDFTYVEN
                     VVQANLKAAEATGAAGQVFNVGNGVSLTLLDLLKELNAIFGKNIQPIFQPARAGDVRD
                     SLADISRARAVLGYEPNVQLAEGLRLTVEYYRGSAK"
     gene            4261..6270
                     /locus_tag="VFB80_23035"
     CDS             4261..6270
                     /locus_tag="VFB80_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36731.1"
                     /translation="MNARALAAAVVAVSVGLPILADELRLAPRQIMDLATSENVRLNL
                     GERGIELERGVLYEDDGAAAGYCYGPNEERLAGTVAIKKVLLIEQPDATKAFLLVAPG
                     GELSAEINGRAVALRPAGKAGQYWQKYEFDPATLVAGRNEFVLSGGGKIWIARDQDFA
                     AGSKAQKSHPNRSAKSTDGGITWNFDRLGTGGDLDGEYYVRVFLEQPQPRGSLTLPVL
                     DAANLSGSLVAPAVKGASPIHVAAKGSGDLGLQFRTGPTAVVDDKSWTKWQPLASAAG
                     EIPQASRYFQIRIVLASRALQDAARLEGLLITAAADPSSSWHSRLRVVECQNPAIVRA
                     SIPFEYEPVDQPRLKTLRAEYKLDDVALGAKSELELISKLAVWSSQRWDKGHLQQQYP
                     PWDAHEILRPAADGSPVGGFCQQYNVVFLQACESFGLIGRAVSIGPGDGQFAGRIRGG
                     HEVVEIWSNQFAKWIYVDGNAAWYFVDDASGIPLSLRELRTRQLAAFAESPAASPPPR
                     LVRLAESRYEWTGLTSWPPFLELRLIPRSDFLEQAAPLPLNQGMRGWFWTGHHVWTDE
                     RSPASLLYGHRVSQPQNWDWTLNQAHLSLTANEVPGELRVEFDTVTPGLDCFLAKLDD
                     QPPKPAAGSFTWTLRPGMNRLEAWPRNRAGREGVRSRVVLDYGGE"
     gene            6626..7699
                     /gene="recA"
                     /locus_tag="VFB80_23040"
     CDS             6626..7699
                     /gene="recA"
                     /locus_tag="VFB80_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZN36732.1"
                     /translation="MAKKERAPAGNGKAAVSSLIEDNATLKTALQQIEKQFGEGSIMP
                     LGGERTLAISGIPTGSLSLDVALGGQGIPRGRIIEVFGPESSGKTTLALHVAAQAQKA
                     GGIAAIIDAEHAFDPTWGKKLGVQLETLLVSQPNNGEEAMQITEMLVKSNSVDVIIID
                     SVAALVPRAELDGEIGDTHVGLQARLMSQSMRKLTGAIAKSKTAVIFINQIREKIGVS
                     YGSPETTPGGRALKFYSSCRIDVRRISQLKDGEEVVGQRVRAKVVKNKVAPPFRFAEF
                     DMMHTCGISYEGDILDLGLNQKILTRSGAWFKYGETYLGQGKEKARAFLLENPQVTAE
                     IREKILASGSLAGGVASEGEAAE"
     gene            7840..9273
                     /locus_tag="VFB80_23045"
     CDS             7840..9273
                     /locus_tag="VFB80_23045"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN36733.1"
                     /translation="MACGAAPLAAQEPSALAAAAAMEKLVTEAIEKAEKSVVAIARVR
                     REPAAEAGSPLRLERTDPLDPAFVPHEFGTGVVIDAKGLIVTAAHVLGNVREADYYVW
                     ISRKPYPAKVKAADPWLDLAVLQIDAANLKPITFGNARELKKGQFVIALGNPYAIAKD
                     GQPSASWGIVSNLERQAPAPRTLTRPSETGETLHHWGTLIQTDARLELGASGGALVNL
                     KGEMVGLTTSLAALYGYERPGGFAFPVDDDFRRALDTLKTGRLPDYGFLGVAPATLAA
                     DLRRQGRFGAKIEDQLGGDGVVPGTPAAAAGLKAGDVITHVEGRPVADSVELIRHVSG
                     LFADSNITLRILRGSDATRPGRPMNVKVKLSKKRVEGPREGFAEVEPPAWRGLRVEYS
                     TASPLFREQSRELDPAGCVGVVEVHRDSAAWKAGLRPGDFVSHVGQSRVTTPGEFYGA
                     VNLIEGEAVLKLTAAPADKATRTVQLD"
     gene            9397..>10141
                     /locus_tag="VFB80_23050"
     CDS             9397..>10141
                     /locus_tag="VFB80_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HZN36734.1"
                     /translation="MKTDEIREKYLSFFQSKGHTRQASDVLVPRWDPSVLFTPAGMNQ
                     FKDHFLGKVKLEFTRATTCQKCLRTGDIDNVGRTAYHHTFFEMLGNFSFGDYFKREAI
                     HWAWELLTDKKWFGINPDQLTVTVYLDDDEAAGIWQKDIGLPASRIERMGEDDNFWPA
                     GAPSKGPDGVCGPCSEIFFKPNGGGKAFEIWNLVFTQFNRVGDPPNNLRPLPSKNIDT
                     GMGLERMAAVMQGVDTNYHIDTLRPLVEAA"
BASE COUNT         1783 a         3360 c         3369 g         1629 t
ORIGIN      
        1 cggcgagtca tttcgtttcc accgacgccg atgcggcgaa cgaacagggc gacgtttccg
       61 ccgagatttc cgcggcgctg ctggaattga cgcagaccag ccgccagcgg caagtcgccg
      121 agcagcggca gaccgaccgg atgcaggagt tgtcgagcga gcccacggcc gccgactggt
      181 cggcggagcc ggccgtggtc gtgacgcggc tgggcggaac gccgcgttcc gtgacgctct
      241 tgctgggcag cctgctggct tgcacggccg cgatcgtcat gttccgtgcg tcttcggccg
      301 cggtcctccc tccaaaaatc cagacggccc gcgagctggc cgcggtgctg gagatcccgg
      361 tcatcggcag cgcggccagc ctgcggaccg cggcggcccg cctgcgccag cggctgttca
      421 agccggtcta cgtgcggctg gcggggcacc tggcggaagg cgtgctcgcc ttggcggccg
      481 cggcgtgcct gatctcgatc acggtggagc cgacgctggc atcgcaggtg ctggcggatc
      541 cgttcggcac gctgtcggaa gtcatggggc gattcgtgag cagccggtag ccggcagact
      601 cgcggcgatc cgccgaatcg ctcggaccgg ccgcgccgtg ctccaggccg ccgccccggt
      661 tggtcgatgg gacagacaag ggaaattgcg ccgctcgagc ttcgcgcgtg agcagcgggt
      721 gctagcagcg cgggggaagg ccaccagatt caggatgccg cgatgtacga gagtttcttc
      781 cggctgacgc atcgcccgtt cgtgcctgcg ccgctggccg aggcttacat cccgaccagc
      841 tcgctcgacc acgcccgcca gacgctcgtc cgctgcctcg accgtggcga ggggcccggg
      901 ctgatcatcg ggccggccgg caccggcaag tcgctgctct gccaggtgct cgccaagcac
      961 tttcgcggcc gctttcagat cgcgctgctc tcggccgcgc ggctctgcac ccggcgggcc
     1021 ctgctgcaga acatcctgtt cgaattgcgg ctgccctatc gcgagctgga cgagagcgag
     1081 ctgcggttgt cgctgatcga ccacctggag ccgcgggccg gcggaaccga ggggctcttg
     1141 ttgctggtcg atgaggcgca cacgctgccg ctgcgactgc tcgaggaaat ccgcctgctg
     1201 acgaacctgg tgcaaggcgg ccagtcgcgg gtgcggctgg tgctggccgg aggcctcgcg
     1261 ctcgaggagc ggctgaacaa tccgaagctc gactcgttcc tgcagcggat cgtggcccgc
     1321 tgctatttgc agccgctggg ccgcgacgag acaatcttct acgtgcagga gcagttccgc
     1381 cgctgcggag cggccgccga cagcgtgttc acgggcgacg cccagacggc gattcacacg
     1441 gcgaccgacg gcattccgcg gctgatcaac cagctgtgcg accacgcgct gatgctggcg
     1501 gccctcggcg gggagcggac gatcgactcg ggcggcgtcg aagaagcctg ggccgacctg
     1561 cagcagctgc cggtcccgct gcacgatccg ccggccacac gcagcacatc gagcggcggc
     1621 agcaccggcg gcatcgtcga attcggccag ctcggcgaag cgccgaacaa tcagaccgtc
     1681 ccgctggccg acgcggcgct ggcgaatatc gacgctatcg accagggctt gagcttgctc
     1741 aagacgagcg aggccgcggc ggaagcggca ggcgaatcaa gcggcgagtt ctcgccggcc
     1801 gatccgagcg gcaccgaagt ggaattgatt ttccattcgt cgcacgatcc gttcggcggc
     1861 cattgggagc acgaggaagt ggtgatcgac cgctacgcgt cgctggaaga tgcggctgtg
     1921 cggaaccggc ggcaagtcac gagcgatgaa ggcagggcga tcggggcggc ggtgagcgca
     1981 gcggcagtcc caggcgagcc ggctcagccg cgcgaggatg aaccggtgag ccagaattcg
     2041 atgacgattc cggcgtcggc cgcgtttcac ccggccagcg atccgctgct gccggaggag
     2101 ccgccgacgc cgcaatcgcg gcgggccacg gtctcgctga atgaattggg gcaggacgac
     2161 cgcgatttga tcgtggtcga agacgatcag gcaggcgccg ttccgccgct cgccggccgg
     2221 ccccggcggc ccgaataccg ccagcttttc agctccttgc ggaataagta gcggtctatg
     2281 gcccgcatgc tccaagccct gaaaaatctg gaagcccggt cgaaaaaacc ggccgcggcc
     2341 ggaggattgt tggggcacct ggccgacaat gccggtcgaa ctaagccgcc cgcgcagccc
     2401 gctgccaacc ctgcgcagcc cgcccccaat ccagcgccgc ccgctcccaa caatacggcg
     2461 ctggtgccgg tctatctgca ggcccaagac gtcgcggcag attcagccgc gcctgtcgtc
     2521 gcagcgccgc ccgccaagcc tcagccggac ccgcctcccg caatcaagcg cgaccgattc
     2581 ggcatcaacc ggccgccggc gaatctcgaa cgcgcggtgc gccgcacgct caccgattcc
     2641 agccgcgctc agccgtttca gcagctggcc caccgcctgg cccgcgacgt cgagcagacc
     2701 gacagcaaga ctttgctctg gatcagcatc ggcgagagcc gtggcggcta tgaatcgctc
     2761 ctgcactcgg cggcgctgct ggccgaggcc ttggagggtc cgacgctgct catcgacgcc
     2821 gacctgggcc gccggtcgct ctcgggcgac ctcgactatg gccagctgac cggcctggcg
     2881 gagttgctga agaacgacga gcggccgcaa ccgctctgcc ggccgaccgc cttcgagcgg
     2941 ctgtcgctct tgccggcggg gcgagcgagt tacgtcgatt tgctggcggg cccgcggctg
     3001 gaaaacctgc tccaggaatt ggccggcgag tattctctcc tcttgatcga cggaggacgc
     3061 tcctccgatg cggctgctgc cgcccttgcc cgcgtctcgg acgccaccta ctttgtagtt
     3121 caactcggcg tcgtcgaagg ctgtgcggcc caggccgcgc tccgcgactt ccgagccgcc
     3181 ggggcgaaag tgctcggctg cgtggcgacg taggaaaact tcaagaacca aagaaccaaa
     3241 gaaccaaaaa ggttacggag cttccgcttc ggttctttgg atcttcggtt ctttggatct
     3301 tcggttcttg gaatttgatt ggcttatgcc tacctgtctc gtcaccggcg gcgcgggctt
     3361 cgtcggctcg catatcgctg aggccctggt ccgccgtggc gacaaggtcc gggtgctcga
     3421 taacttcgac acgggcaagc gcgagaactt gcaggccgtc ctcggccagg tcgagctgat
     3481 tactggcagc gtggccgatg cagcggccgt ggccaaggcg gtcgccggct gcgaattcgt
     3541 gtttcaccag gcggcgctgg cctcggtgcc gcggagcatc gacgatccgc tggcgacgca
     3601 cgccgcgtgc gcgaccggca cgctgaacgt gctgaacgag gcccgcaagg ccaaggtccg
     3661 ccgcgtcgtc tatgccgcca gcagcagcgc ctacggcaat cagccgcagc cccagaagcg
     3721 cgagaccgat ctgccctcgc cgctgtcgcc ctatgccgcg gccaagctgg cggcggagct
     3781 gtattgcgag gccttctggc acagctacgg cctggaggcg gtcgccctgc ggtacttcaa
     3841 cgtcttcggc ccgcggcagg atcccagcgg cccgtacgcg gcggtgatcc cgatttttat
     3901 ccagaagatt ctcgccgggc agcagccgac aatctttggc gacggcctgc agacgcgcga
     3961 cttcacgtac gtcgagaacg tcgtgcaagc aaacctcaaa gcggcggaag ccactggtgc
     4021 cgcggggcag gtgttcaacg tgggcaacgg ggtctcgctg acgctgctcg acctgctgaa
     4081 ggagctgaac gccatttttg ggaagaacat ccagccgatc tttcagccgg cccgcgcggg
     4141 cgacgttcgc gacagtctcg cagacatcag ccgggcccgc gcggtgctcg gctacgagcc
     4201 caacgtgcag ctcgccgaag gactgcgact caccgtcgag tactatcgcg gatcggccaa
     4261 atgaacgcgc gcgccctcgc ggctgcggtt gttgcggtct cggtcggact gccgatcctg
     4321 gccgacgagc tgcgtctggc gccccggcag atcatggacc tggccacgag cgagaacgtg
     4381 cgcctgaact tgggcgaacg gggaatcgag ctcgaacgcg gcgtcctgta cgaagacgac
     4441 ggcgccgcgg ctggctactg ttacggaccc aacgaagagc ggctggcggg caccgtcgcg
     4501 atcaagaaag tcctgctgat cgagcagccg gatgcgacca aggcgtttct gctggttgct
     4561 ccgggcggcg agctgtcggc cgaaatcaac ggccgcgcgg tggcgcttcg tccggccggc
     4621 aaagcgggtc agtattggca gaagtacgag ttcgatcccg cgacgctggt cgctggccga
     4681 aatgagttcg tgctgtccgg tggcggcaag atctggatcg cccgcgacca ggatttcgcg
     4741 gcggggtcca aggcgcagaa gagccacccg aatcgcagcg ccaaaagcac cgacggtgga
     4801 ataacctgga atttcgatcg cctggggacc ggcggcgacc tcgacggcga atactacgtc
     4861 cgcgtctttc tcgagcagcc gcagccacgc ggcagcttga cgctgccggt gctcgacgcg
     4921 gcgaacctga gcggctcgct ggtcgcgccg gcggtgaaag gcgctagccc catccacgtc
     4981 gccgccaaag ggagcggcga tctgggactg caattccgca cgggaccgac agccgttgtg
     5041 gacgacaagt cttggaccaa atggcaaccg ctggcgagcg ctgccggcga aattccgcag
     5101 gccagtcgct actttcagat acgcatcgtc ctggcgagcc gagctcttca ggacgcagcg
     5161 cgactcgaag ggctgctgat caccgccgcg gcggacccga gttcctcctg gcactcgcgg
     5221 ctgcgagtcg ttgagtgcca gaatccggcc atcgtccgcg cttcgattcc atttgagtac
     5281 gagcccgtcg accagccgcg gctgaagacg ctgcgcgcgg agtacaaact ggatgacgtc
     5341 gccctgggag ccaagagcga attggagttg atctcgaagc tggcagtctg gtcgtcgcag
     5401 cgctgggaca aggggcactt gcagcagcag tatccgccgt gggacgcgca cgagattctg
     5461 aggcccgccg ccgacggatc gccggtcggc ggtttctgcc agcaatacaa cgtggtgttc
     5521 ctgcaagcct gcgaaagctt cggcctgatc ggcagggccg tttcgatcgg gcccggcgac
     5581 ggccagttcg ccggcagaat tcgcggcggc cacgaggtcg tcgaaatctg gtccaaccaa
     5641 ttcgccaagt ggatctacgt cgatggcaac gccgcgtggt acttcgtcga tgacgcatcc
     5701 ggcatcccgc tgtcgctgcg cgagctgcgc acgcggcagc tcgctgcttt cgccgaatct
     5761 ccagccgcat cgcctccgcc gcgcctcgtt cgcctcgccg agtcccgcta tgagtggacg
     5821 ggcctcacca gttggccccc cttcctcgag ctccggctga tcccgcggag cgacttcctg
     5881 gagcaggccg cgccgttgcc gctcaatcaa ggtatgcgcg gctggttctg gaccgggcat
     5941 cacgtctgga ccgacgagcg ctcgccggca tcgctgctct atggccaccg cgtcagccag
     6001 ccgcagaatt gggactggac gctgaaccag gcgcaccttt cgctcaccgc caatgaggtt
     6061 ccaggcgagt tgcgagtcga atttgacacg gtcacgcctg gcttggattg ctttctggca
     6121 aaactggacg atcagccgcc caaaccagcg gccggatcct tcacttggac gctgcggccc
     6181 ggaatgaacc gcctggaagc ctggccgcgg aaccgcgcag gccgcgaggg cgttcgcagc
     6241 cgggttgtgc tggattacgg cggcgagtga acggtagtgt ggtcaagcga gtccgcgggc
     6301 gcagacccac gcggctgtgg agcgtcgggc gccgcattgc ggtctaatag aaaataggaa
     6361 gcctgtctgt gctgcggctg cctcgcgaac tatttcgata aatctttttt tcgcttgact
     6421 caaatggacc agactagtcc aaagcctcgc gacaatggtc gtaacgagtc gcgagtggtt
     6481 gacgggcaac tagaacgggc gtacacttct cgcctgttca cgtgaagccg gaaacgcctc
     6541 gcgacttgca tcgccctatc tggtccccaa taaaggaggt cacgtcatgg cagccactct
     6601 ggaaaaatcg aactcgaatc aacgtatggc caaaaaggaa cgcgcgccgg cgggcaatgg
     6661 caaggcggcg gtctcgtcgc tgatcgaaga caacgccacg ctgaagaccg ccctgcagca
     6721 gatcgaaaag cagttcggcg aaggctcgat catgccgctg ggcggcgagc gcacgctggc
     6781 gatttcgggc attccgaccg gcagcctctc gctcgacgtc gccctgggcg gccagggcat
     6841 tccccgcggc cgcatcatcg aggtcttcgg cccggaatcg agcggcaaga cgacgctcgc
     6901 cctgcacgtc gcggcccagg ctcagaaagc cggcgggatc gcggcgatca tcgacgccga
     6961 gcacgccttc gatccgactt ggggcaaaaa gctgggcgtc cagctggaga cgctgctcgt
     7021 cagccaaccc aacaacggcg aggaggcgat gcagatcacc gagatgctgg tgaagagcaa
     7081 ctcggtggat gtgatcatca tcgattcggt cgccgccctc gtcccgcggg cggagctcga
     7141 cggagagatc ggcgatacgc acgtcgggct ccaggcccgc ctgatgagcc agtcgatgcg
     7201 caagctcacc ggggcgatcg ccaagagcaa gaccgccgtg atcttcatca accagattcg
     7261 cgagaagatc ggcgtcagct acggcagccc ggaaacgacg cccggcggcc gggcgctcaa
     7321 gttctattct tcctgccgca tcgacgtccg ccggatcagc cagctcaagg acggcgagga
     7381 agtcgtcggc cagcgggtgc gggccaaggt cgtcaagaac aaggtcgccc cgccgttccg
     7441 cttcgccgag ttcgacatga tgcacacctg cggcatcagc tacgaagggg acatcctcga
     7501 cctgggcctg aaccagaaaa tcctgacccg cagcggcgcc tggttcaaat atggcgagac
     7561 ctacctgggc cagggcaagg aaaaggcccg cgcctttttg ctggaaaacc cccaagtaac
     7621 cgccgagatt cgcgaaaaga tcctggcctc cgggtccctg gccggcggcg tcgccagcga
     7681 gggcgaggcg gccgaatagg gttgcggcat tcgccacttg ctatcgacaa tgaaaagccg
     7741 ggagcggagt tgctcccgtg cttgtcgtca ctcggcggac ggagcgttat cgccccggtt
     7801 gttatgcgtt tctccgcgat ttgcctggtt ggatcgctaa tggcctgcgg cgcggctccg
     7861 ctcgccgctc aggagccctc ggccctggcg gctgcggcgg cgatggaaaa gctcgtcacc
     7921 gaagcgatcg aaaaggccga aaaatcggtc gtcgcgatcg cccgtgtccg ccgcgaaccc
     7981 gcggccgaag ccggctcgcc cttgcggctg gaacggactg acccgctcga tccggcgttt
     8041 gtcccgcacg agttcgggac gggcgtcgtg atcgatgcca aggggctgat cgtcacggcg
     8101 gcccacgtgc tgggcaacgt ccgcgaggcc gactactacg tctggatcag ccgcaagccg
     8161 tatccggcca aggtgaaggc cgccgatcca tggctcgacc tggcggtgtt gcaaattgac
     8221 gccgcgaacc tgaagccgat cacgttcggc aacgcccgcg agctcaaaaa gggccagttc
     8281 gtcatcgccc tgggcaatcc ctacgccatt gccaaggacg ggcagccgag cgcctcgtgg
     8341 ggaatcgtca gcaacctgga gcggcaagcc cccgcgccgc ggacgctcac gcggcccagc
     8401 gaaaccggcg agacgctgca ccactggggc acgctgatcc agaccgacgc ccggctggag
     8461 ctcggcgcaa gcggcggggc gctggtcaac ctcaagggtg agatggtcgg cctgacgact
     8521 tcactggccg ccctctacgg ctatgaacgc ccgggcggct tcgcctttcc cgtcgatgac
     8581 gatttccgcc gcgcgctgga cactttgaaa acgggacggc tgcccgacta tggcttcctc
     8641 ggcgttgcgc cggcgacgct cgcggccgac ctgcggcggc agggccggtt cggagcgaag
     8701 atcgaggacc agcttggcgg cgacggcgtc gttccgggca cgccggccgc ggcggcggga
     8761 ctgaaagcgg gcgacgtgat cacgcacgtc gaaggccggc ccgtcgctga cagcgtcgag
     8821 ctgatccgcc acgtcagcgg cctgtttgcc gattcgaaca tcacgctgag aatcctccgc
     8881 ggcagtgacg cgacgcggcc gggccgcccg atgaacgtga aagtcaagct ctcgaagaag
     8941 cgcgtggaag gtccccgcga gggtttcgcc gaagtcgagc cgcccgcctg gcgcggcctg
     9001 cgcgtcgaat actcgaccgc ctcacctctg ttccgcgagc agagccgcga gctcgatccc
     9061 gccggctgcg taggcgtcgt cgaagtccat cgcgactcgg ccgcctggaa agctggcctg
     9121 cgtcccggcg acttcgtcag ccatgtcggc cagtcccgcg tcacgacgcc cggcgagttc
     9181 tatggcgccg tgaacttgat cgagggcgaa gcggtcctca agttgaccgc cgcccctgcg
     9241 gacaaggcga cgcggaccgt gcagctggat tagctccggc tattcggtat ttcgcgcctt
     9301 cgctatgctt cgtttaaccc ggagccggag gcgaccgcgc atttgcacaa cgccgtcgcc
     9361 tccggctccg ggttaaacga catacacaga ctccccatga aaaccgacga aatacgcgaa
     9421 aaatacctga gtttctttca atcgaagggg cacacccgcc aggccagcga cgtgctggtg
     9481 ccgcggtggg atccgtcggt gctgttcacg ccggcgggca tgaaccagtt caaggaccac
     9541 ttcctgggca aggtcaagct ggagttcacg cgggccacga cctgccagaa atgcctccgc
     9601 acgggcgaca tcgacaacgt gggccgcacg gcctatcacc acacgttctt cgagatgctc
     9661 ggcaacttca gcttcggcga ctatttcaaa cgcgaggcga tccactgggc ctgggagctg
     9721 ctcaccgaca agaagtggtt cggcatcaac ccggaccagc tcacggtgac agtctatctc
     9781 gacgacgacg aagcggcggg catctggcaa aaggacatcg gcctgccggc gagccgcatc
     9841 gagcggatgg gcgaggacga taacttctgg ccggccggcg cgccgagcaa aggccccgac
     9901 ggcgtctgcg gtccgtgcag cgagatcttc ttcaagccca acggcggcgg caaggcgttc
     9961 gagatctgga atctcgtctt cacccagttc aatcgcgtcg gcgatccgcc caacaacctc
    10021 cggccgctgc ccagcaagaa catcgacacc ggcatgggac tggagcggat ggccgcggtg
    10081 atgcaggggg tcgatacgaa ctaccatatc gacacgctcc gcccgctggt cgaagccgcc
    10141 g
//
LOCUS       DASRJB010000878        13899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1386164, whole genome shotgun sequence.
ACCESSION   DASRJB010000878 DASRJB010000000
VERSION     DASRJB010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386164"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VFB80_23055"
     CDS             <1..1782
                     /locus_tag="VFB80_23055"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HZN36735.1"
                     /translation="FRYRDWVIAAVNADLPYDRFSIEQLAGDLLPEASQEQLLAVGLH
                     RNTLHNTEGGTDPEEDRVKKTVDRTNTLGAIWLGLTVGCAQCHSHKYDPLSQREYYSL
                     FAFFNSFDEKDLETPTPEQAAALEKARDAHKQKQQALEAAVTNYVAEKLPAAQTEWES
                     TLAGKSPEQLAEQKIPAGVAAALSKPSAERSTAESQQIADHYRSLDKQLVKLEKAVTD
                     HKAKPPQLPDEARAQSVSELQQPRETHLLLRGDFLSPGDAVPAGTPAVLPPLATENRA
                     SRLDLARWLFDPAQPLTARVAANRVWHQLLGRGIVPTLDDFGKQGEKPSHPELLDWLA
                     SELAAAGWSQKALIRRIVQSATYQQSSAPRGDLTRRDPENVLVARQVRRRVESELIRD
                     LALAASGLMTHQIGGPSVRPPQPAEYAGLTYANSAKWVESKGADRYRRGLYTFFQRTS
                     PYPMLITFDSPDSNECTAGRQTSNTPLQALTLWNDPAFFEAAQALGRRVVAEVPAESD
                     ERETTRLRAERAFLLCLSRRPTSAELASVIDLHAASRQLAARDEAAARQLVGSPPIPS
                     SDDITEIAAWVSVARALINLDEFISRE"
     gene            1860..2249
                     /locus_tag="VFB80_23060"
     CDS             1860..2249
                     /locus_tag="VFB80_23060"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZN36736.1"
                     /translation="MLLHQELSRSIIGAAMTVLNELRPGLDEKLYERALVIELLARGH
                     RVESQRPFEVYYKERLVGTLVPDLIVDELVIVDPKVVTEFCDSHLAQMLGYLNITKLQ
                     LAILLNFKYSKLKWKRVVQEAMVEQYE"
     gene            2501..3985
                     /locus_tag="VFB80_23065"
     CDS             2501..3985
                     /locus_tag="VFB80_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36737.1"
                     /translation="MNCQFDHARRQFFTSSASGLGVLALASLLRQDGLLGAEGGSSSA
                     NLLAPKAPHFAPKAKSCIFFLPEGAPSHIDLFDPKPKLRELHGKPLPESMTKNVRFAF
                     IKKETAVLWGSKRTFTPHGECGMELADFLPRIGSCADDICLIRGMHTDSFNHHPAQLM
                     LMSGVPRFGRPSMGAWLNYGLGSESENLPGYVVLTAGRGGSGGVSNWTSGFLPSTYQG
                     VLFRDQGDPVLNLANPPGVSSELQAQSLGAISALNRQRLEALHDPEIASRIAAYELAF
                     RMQSAAPELTDLSQETQATLDMYGCDRPEPTDLGGGGRSGGKDVFKQFARNCLLARRL
                     VERGVRFVTLVHASWDHHSNLDEELGYAARMADQPMAALVQDLKQRGLLDSTLLVFSS
                     EFGRTPLAENRGGKMPEKVGRDHHPFAYSLWLAGGGFKGGTVYGKTDEIGWSVAENPV
                     HVNDLHATILHQFGFDHRRLAVNYKGLSVRLTDQGGQVIRDLLA"
     gene            complement(4867..6345)
                     /locus_tag="VFB80_23070"
     CDS             complement(4867..6345)
                     /locus_tag="VFB80_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN36738.1"
                     /translation="MIACARWAIAALIAVSPCLVQAADPPRPNIVLLFADDLGYGDLG
                     CYGAQGIATPNLDRLAAQGTRFTSFYVAQPVCTASRAALMSGCYSNRISLFGALNHES
                     NVGIAADERLLPEMLKDAGYATAIFGKWHLGHREAFSPLKHGFDEFLGLPYSNDNGPL
                     HPVVRTIPGLPLIEGDKVAETDPDQSQFTRRFTDRAIAFIEKNKDRPFFLYVPHVMPH
                     VPIFASAEFRGKSSAGLYGDVVQELDAGVGDVLAALDRLKLSEKTLVIFCSDNGPFLS
                     YGNHAGSAGRLREGKLTTFEGGVRVPCLMRWPGKIPAGRVSPDLLSTLDLLPTFAGLA
                     GGKISTNKIDGEDRWQLIAGLPDARPRETFCYYAGDELQAVRSGRWKLHLPHEYLSPA
                     QPPGKNGKPANFENLKPESMAMSGLRGIASRHGYVVKQIGLTLYDLETDIGETTDVAA
                     QHPEIVARLQKLAESARADLGDSLTKRKGAGVRPCGTVSPPR"
     gene            complement(6362..7294)
                     /locus_tag="VFB80_23075"
     CDS             complement(6362..7294)
                     /locus_tag="VFB80_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN36739.1"
                     /translation="MIKTHNLTKKYGESYAIRAIELDLAAGDLFGFIGPNGAGKTTTM
                     KILATLLGATSGEAYVCGHSIYRDPKEIRRLVGYMPDFFGVYDDMKVIEYLEFFAAAY
                     RISGPKRRQVCDEKLQLVDLDFKRDAYANTLSRGQTQRLGLARVLLHEPQVLLLDEPL
                     SGLDPRARIEMRAVLRRLGQQGKTIMVSSHILPELADMCNKVGIIDRGVMTVNATVAD
                     VMRQVRERIVLQIVVTGDQAAAAKLLEQHELVQSVAINKEMLAVTLKSSITDYCDLSS
                     LLVGAGHKLKLFREEEVNLESAFMALTKGLGQRV"
     gene            complement(7328..9514)
                     /locus_tag="VFB80_23080"
     CDS             complement(7328..9514)
                     /locus_tag="VFB80_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36740.1"
                     /translation="MSSLRQFVPAALLAALFAASLPVGLAAGDDLLTVKASSVGFGGK
                     FKSGFWQPVRATLVAGETSVRGRLEVVAADGDQTPVIYANEQAPELNLSPQQETTVLL
                     YAKSGPIAALLVLQLRTDAGVVWSQDLATPTIGATQEFVVGIGPSAGLDDAAATLRRR
                     SEATLVTAQVQSAAELPDVWWAYSGVDAVLLTTSDAKFLESMSAAQRQALIDWVLLGG
                     RLVLAAGARGAEITAAGSAWQTLVPGDFEEVVPLRERAGLETFTRTELPFQAEFFQRN
                     RPLVTRLKNVRGEVLLGEASALATRPLAVHAPAGLGEVTFVALDLDHPSLAAWEGRSR
                     LIAALIQRDRSSLDADERQRGSGVGHLGYSDLVGQLRLALEQFSGVTLVNFTTVAVLT
                     IVFLLIVGPGDYLLLSRTGWPRQLTWITFPLLMLAATLAFGYISRQAHGTRTRLNLVE
                     IVDFDVDRGLVRGTVWAHLYSPRTARYDVYLHLPAAAGSPHGWLSWQGLPGEALGGLG
                     SRQVVLASVEPYRVNMPGPAPATLDLPTTIASSKSLSAQWWAKTPLTVESKLALDEFG
                     LVGGEFTQPLPVELSDCLLAHGEKLFRLGTLQPGARVVVDPQRALNLEWRLTERRVEQ
                     SKDVTTPWNQAATDVPQIVQMLMFHEAAGGRSYTGLSHRYQPQIDLSEHIRLGQAVLV
                     GRAKQPVAGLGLGARAGEAAPLAAAADTTTWTWYRLILPVSPHTKR"
     gene            complement(9622..9885)
                     /locus_tag="VFB80_23085"
     CDS             complement(9622..9885)
                     /locus_tag="VFB80_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HZN36741.1"
                     /translation="MYFEKDQARGVEGTFLWLMEEVGELATALRSGTHQERLEEFADV
                     LAWLATIANVAGVDLSEAIAQKYGRGCPGCGKFVCTCPDSEKP"
     gene            complement(9939..12752)
                     /locus_tag="VFB80_23090"
     CDS             complement(9939..12752)
                     /locus_tag="VFB80_23090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36742.1"
                     /translation="MRVGVAIFVCAALFAPTIAAADDSLPPRTLLLKGRYDEAVAEFA
                     KSAGEDPAAAIGLARCRLATGRRDDAAAGLTAAAEKLPKSAAIHGELALLALRRGDHD
                     AAAREAAAAVALDKDCVSAHWVQAELLRLSGKISEAQSAYAWFIGYHNRAPQIADPMD
                     LLLIGRAVAEHARWTRNSNQFRRLVNDVYPSALRRASDFWPAHFEAALLYLEKYNEAE
                     ATAEIDRGLAINPHAAELHAAKAALALSRFNLAAAKSSLDRALEINPELIWAHQLRAD
                     SLLADLRPAEAIPILEKALQLNPRDEATLGRLLAAHTIAGPPHKVEQIASGATRNNPH
                     CGEVYLAAAESFDRMRRFTRAAEYYRLANDKLPQLISARGELGLTLMRLGEEAEAAKL
                     LNESFAIDPFNVRVKNMLEVLDVLKDYATIETEHFVIRFDRGQDELLARYASRHLEEE
                     VFPQLTRLLGYTPEGKTLIEIFNRSGRTSGHSWFSARMVGLPFIHTVGACAGKMVALT
                     SPGELPEKYDWATVLRHEFVHVLNLQQTDFGVPHWLTEGLAVHLEAQPRPKKWTQLLA
                     SRARSGELFDLDTLTLGFIRPQSSDDWTLAYCQAELYVEFLLATHGEDAIAKLLAAYR
                     DRLTTADALARCYGVKQADLEAKYKEYVSKQIEGAGKLIPREKPKFADLERKVEAGPP
                     DADLAAEFALACLDRGLAADARKWALEAQKQKPRHGGAAYVLARQQMSIGDFDAAVKL
                     LENGLDKSALHEEALALLAAVKLKDGDFKTAEELYQLGDQQFPGSDRWVTGLARVYLQ
                     PGGLPDAEAKLLPVLRRWSQAEPDNLTIRQKLAEVSLAQKDYGAAAGAALEIIHRDVE
                     YAAGHALLAAALAAQEKNAAAVDEYQTALRLDGKQPAWQAALARLLIGLGKKEEAGAA
                     IERLRELDPKHPQIAELEKSLTQ"
     gene            12840..13178
                     /locus_tag="VFB80_23095"
     CDS             12840..13178
                     /locus_tag="VFB80_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN36743.1"
                     /translation="MTEFVTVAKVDEIPAGEGRAFAVNGRMVAVFNDEGRYFSIDDFC
                     PHMGASLAGGWLEKGTVTCPWHAWRFCIHDGKWLDNPKVGVDAFEVRLAGDEIQVKVP
                     PKQVPPLPGT"
     gene            13535..>13899
                     /locus_tag="VFB80_23100"
     CDS             13535..>13899
                     /locus_tag="VFB80_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36744.1"
                     /translation="MIAANNNWRVAVWLARFRGDQSLAERNCELTMNSLKSATGLTFI
                     LIVTGSLWGQAPRAAENQLNAGRQTGWQLVQLVVEKLDAGPGGEFPGIAAWKQDFATA
                     TRGIDAAAPADKWPAVAMDA"
BASE COUNT         2444 a         4511 c         4512 g         2432 t
ORIGIN      
        1 ttccgctatc gcgactgggt gatcgccgcg gtcaacgcgg acctgcctta cgaccggttc
       61 tccatcgagc aactcgccgg cgacttgctg ccggaggcgt cgcaggagca attgctggcg
      121 gtcggcctgc accgcaacac gctgcacaac accgaagggg gcaccgatcc cgaggaggac
      181 cgcgtcaaga agacggtcga tcgcaccaac acgctcggag cgatctggct ggggctgacg
      241 gtcggctgcg ctcagtgcca ctcgcacaag tacgatccgc tctcgcagcg cgagtattac
      301 tcgctcttcg cgtttttcaa cagttttgac gagaaggact tggaaacgcc aactcccgag
      361 caggctgccg cgctcgagaa ggcccgcgat gctcacaagc agaagcagca ggcgctggaa
      421 gcggccgtga cgaactatgt ggcggagaag ctgcccgccg cccagaccga atgggaatcc
      481 accctggccg gcaagtctcc cgaacaatta gcagagcaga agattcccgc cggcgtcgcc
      541 gcagctctga gcaagccgtc ggccgagcgg tccacggccg agtcgcagca aattgccgat
      601 cactatcgct cgctcgacaa acagctcgtc aagctggaga aggccgttac cgaccacaag
      661 gccaaaccgc cgcagctgcc agacgaagcg cgggcccagt cggtcagcga gctgcagcag
      721 ccgcgggaaa cgcacctgct gctaaggggc gacttcctca gccccggcga cgcggtgccc
      781 gcaggcacgc ccgctgtgtt gccgccgctg gcaactgaaa acagggccag tcgcctggac
      841 ctggcccgct ggctgttcga tccggctcag ccgctgacgg cccgcgtggc ggccaaccga
      901 gtctggcatc agctgctggg ccgcgggatc gtgccgacgc tcgacgactt cggcaaacag
      961 ggcgagaagc cttcgcaccc ggagctgctc gactggctgg ccagcgagtt ggccgccgcc
     1021 ggctggagcc agaaggcgct gatccggcgg atcgtgcagt cggcgaccta tcagcagtcg
     1081 tcggccccgc gcggcgattt aacgcggcgc gatccggaaa acgtgctcgt cgcgcggcag
     1141 gtgcggcggc gggtcgagtc ggaactgatc cgcgatctgg ctttggccgc cagcgggctg
     1201 atgacccacc agatcggcgg gcctagcgtg cggccgccgc agccggcgga gtatgccggc
     1261 ctgacctacg ccaacagcgc caagtgggtc gagagcaaag gagccgaccg ttatcgccgc
     1321 gggctgtaca cgtttttcca acggaccagc ccctacccga tgctgataac gttcgactcg
     1381 cccgacagca acgagtgcac cgccggccgg cagacgagca acacgccgct gcaggcgttg
     1441 accttgtgga acgatcccgc gttcttcgaa gccgcgcagg cgctcggccg ccgcgtcgtg
     1501 gccgaagtcc ccgccgaaag cgacgagcgc gaaaccacgc ggctgcgagc cgagcgggcc
     1561 tttctgctct gcctctcccg gcggccgacg tcggcggagt tggccagcgt gatcgatctg
     1621 catgcagcca gccggcagct tgccgcacgg gacgaagcgg cggcccggca actcgtcggc
     1681 tcgccgccga ttcccagctc cgacgacatt accgagatcg ctgcctgggt cagcgtcgcg
     1741 cgggccctga tcaacctgga cgaattcatt tcgcgagagt gataggaccg cggggattta
     1801 accgcggata actcggatgg cgcggataag agagatgacg aatttgtgga agagatgcga
     1861 tgttgctcca tcaggaactt agccggtcga ttattggcgc ggcgatgact gtcttgaacg
     1921 aactgcggcc gggattggat gaaaagctct atgagcgagc tttggtgatt gaattgctcg
     1981 cgcgcggaca tcgcgtcgaa tcgcagcgac cgtttgaggt gtactacaaa gaacggctgg
     2041 ttggcaccct ggtgcccgat ctgatcgtcg atgagctggt cattgtcgat ccaaaggtgg
     2101 taacggagtt ttgcgattcg catctggcac agatgctcgg ctatctcaat atcacgaagc
     2161 ttcagctcgc gatcctgctc aatttcaaat actccaagct gaaatggaaa cgtgtggtgc
     2221 aagaggctat ggtcgaacag tacgagtgat ttcaccaacc ctgcccttat ccgcgccatc
     2281 cgagttatcc gcggttcctc cgatgaattg ccaattcgac ctcgcccgga gaagatggaa
     2341 cgcggaaatt ggaaccgcgg ataacgcgga tggcgcggat aataaataaa tgtccgaatt
     2401 tctcgcacat taccttacct ccgaccctgg caacgaaaac ggcttctagt caccaactct
     2461 gctctcatcc gcgccatccg agttatccgc ggttcctcca atgaattgcc aatttgacca
     2521 cgcccgccga cagtttttca cgtccagcgc ctccggcctg ggcgtgctgg cgttggcgtc
     2581 gctgctgcgg caggacggct tgctcggagc ggagggcggt tcctccagtg ccaacctatt
     2641 ggcgcccaag gccccgcact tcgcgccgaa ggccaagagc tgcatcttct ttctgccgga
     2701 aggtgcgccg agccacatcg acctgttcga tccgaagccg aagctgcgcg agctgcacgg
     2761 caagccgctg ccggagtcga tgacgaagaa cgtgcggttc gcattcatca agaaagagac
     2821 ggcggtgctg tggggcagca agcggacctt cacgccgcac ggagagtgcg ggatggagct
     2881 ggccgacttc ctgccgcgga ttggctcctg cgccgacgac atctgcctga tccgcggcat
     2941 gcacaccgac tcgttcaacc accatccggc ccagctcatg ctgatgagcg gcgtgccgcg
     3001 gttcgggcgg ccgagcatgg gcgcctggct gaactacggc ctcggcagcg agtcggagaa
     3061 tctgccgggc tatgtggtgc tcaccgccgg ccgcggcggc agcggcggag tctcgaactg
     3121 gaccagcggc tttctgccca gcacttacca gggcgtgctc tttcgcgatc agggcgatcc
     3181 ggtgctcaac ctggccaatc cgccgggcgt gagcagcgag ttgcaggccc agagcctggg
     3241 cgcgatctcg gccctcaatc gccaacggct ggaagccctg cacgatccgg aaatcgccag
     3301 ccgcatcgcc gcctacgagc tggcgttccg catgcagtcc gccgcgccgg agctgaccga
     3361 cctctcgcag gagacgcagg ccacgctcga catgtacggc tgcgaccggc ccgagccgac
     3421 cgacctgggc ggcggcggac gcagcggcgg aaaggacgtc ttcaaacagt tcgcccgcaa
     3481 ctgcctcctg gcccgtcgcc tggtcgagcg gggcgtgcgg ttcgtcacgc tggtccacgc
     3541 ctcgtgggat catcacagca atctcgacga ggagctgggc tacgccgcgc ggatggccga
     3601 tcagccgatg gccgcgctcg tccaggacct caagcagcgg gggcttctcg acagtacgct
     3661 gctcgtcttc tccagcgaat tcggccgcac gccgctggcc gagaaccgcg gcggcaagat
     3721 gcccgagaag gtcggccgcg accatcaccc gtttgcctac agcctatggc tcgccggcgg
     3781 cggcttcaaa ggcggcaccg tttacggcaa gaccgacgag atcggctggt cggtggccga
     3841 gaaccctgtc cacgtcaacg acctgcacgc cacgatcctg caccagttcg gcttcgacca
     3901 ccgccgcctg gccgtcaact acaaaggcct cagcgtgcga ttgaccgacc agggcgggca
     3961 agtcatccgc gatctcttgg cctaaatgga cccgcgtaat cagttgggtc gtagccgaac
     4021 tcgccagagt tcggattgcc tggagaaaat tgacaaagcc cgcggccagt tgccaggcgg
     4081 ccgacattgt tcgccgcgtt gcgaggctct gtggcgaatt attcggacct gattggccgt
     4141 attcgcaggc tttttcgcat tttgagtccc gattaaatcg ccatttgagc ccgatacgca
     4201 gggatctggc gaacaatgct cccgctttgg cggcaagtct atttgccgca gccacttgcg
     4261 gcaaaaatcg cgcggcgaac aattcgctgt gcgggtgaga cccgcggtcg gcggatcggc
     4321 ggggtcgctt ggccgcgcgc cgaacggcgg caatgcaaac gcattgttca ccgcatgtgc
     4381 gaaacaacag gcgggtggtg aagaattccg agtgcggaat tgcaaaacgg agtggctcat
     4441 ctgagtcctg caatctgcat tctgaatacc gcggaccagc gcgctgctcg tgcagcgatt
     4501 tatgttcaga gcggaaaaca caatgtggac atgattatac actcgcttcc gttggatgcc
     4561 gggcgagaag gcggaaaact gccgctcccg acgagcgcaa accgaggtgc ggcaagtgtt
     4621 tggaatgatc ttgcaaaaaa tgcgggcggc gcgcggcttt ccgacatctg ccgactggga
     4681 attcagtttt cccttgcaat tagtggcagt ttcggccttt tccaaaagag gggcaccctt
     4741 gctgtcccct gacacggcgg ctaccagcca cgccagttgg atcgaattcg aattcccaga
     4801 gtttggattt cctgcaagcc accgagttct ggcgaattcg gctacccaat ctgcccgcgg
     4861 ttcgacttag cgcggcgggc ttaccgtccc gcaagggcgg acgcccgcgc ccttccgctt
     4921 cgtcagcgaa tcgccaaggt cggcgcgggc cgattcggcg agcttttgca gccgggcgac
     4981 gatttcggga tgctgggcgg cgacgtcggt tgtttcgccg atgtcggttt ccaggtcgta
     5041 cagcgtcagg ccgatctgct tgacgacata cccgtggcgg ctggcgatgc cgcggaggcc
     5101 cgacatggcc atcgattcgg gcttcagatt ttcgaaattg gcaggcttgc cgttcttgcc
     5161 gggcggctgt gccggcgaga gatattcgtg cggcaggtgc aacttccagc ggccgctgcg
     5221 gacggcctgc aactcgtcgc ccgcgtagta acaaaacgtc tcgcgcggcc tggcgtccgg
     5281 caatccggcg atcagctgcc agcggtcttc gccgtcgatc ttgttcgtcg agatcttgcc
     5341 gccggccagg ccggcgaacg tcggcaagag gtcgagagtg ctgagcaggt ccggcgaaac
     5401 acggcccgcg ggaatcttgc cgggccagcg cataaggcag ggcacacgca cgccgccttc
     5461 aaatgtcgtc agcttgcctt cccgcaagcg accggccgaa ccggcgtggt tgccgtacga
     5521 gagaaacggc ccgttgtcgg agcagaagat gaccagcgtt ttctcactca gcttcagccg
     5581 gtcgagcgct gccagcacgt cgccgacgcc ggcgtcgagc tcctggacca cgtcgccata
     5641 cagacccgca ctcgatttgc cgcgaaattc cgccgaagcg aagatcggca cgtgcggcat
     5701 cacgtgcggc acatacagga agaacggccg gtctttgttc ttttcaatga acgcgatcgc
     5761 ccgatcggtg aagcggcggg tgaactgcga ctggtcgggg tcggtctcgg ccaccttgtc
     5821 gccttcgatg agcggcaagc ccggaattgt ccggacgacc ggatggagcg ggccgttgtc
     5881 gttcgaataa ggcagaccga gaaactcgtc gaagccgtgc ttcagcggcg agaatgcctc
     5941 gcggtggccc aggtgccact tgccaaaaat cgccgtggca tagcccgcgt ccttgagcat
     6001 ctccggcagc agccgttcgt ccgcggcgat ccccacgttc gactcgtggt tgagcgcccc
     6061 gaacaggctg atccggttgg agtagcagcc gctcatcagg gccgcccgcg acgccgtaca
     6121 aacgggctga gcgacgtaga agctggtgaa gcgcgtgccc tgcgccgcca ggcggtcgag
     6181 gttcggcgtt gcaataccct gtgcaccata gcagccgagg tcgccgtagc ccagatcgtc
     6241 ggcaaacagc aggacgatgt tcggccgcgg cggatcggcg gcctgaacca ggcacggaga
     6301 cacagcaatc agtgcggcga ttgcccagcg agcgcacgcg atcatgaaat ggcctcctgg
     6361 atcagactcg ctgccccagc cccttggtca gggccatgaa ggcggattcg agattgactt
     6421 cttcttcgcg gaacagtttc agcttgtgcc cggcgccgac gagcaagctg gagaggtcgc
     6481 aataatcggt gatgctggac ttcagcgtga ccgcaagcat ctccttgttg atcgcgaccg
     6541 actgcaccag ctcgtgctgc tccagcagct tcgccgcggc ggcctggtcg ccggtgacga
     6601 cgatctgcag cacgatccgc tcgcggactt gccgcatcac gtcggccacg gtggcgttga
     6661 ccgtcatcac gccgcgatcg atgatcccga ccttgttgca catgtcggcc agctccggca
     6721 ggatgtggct cgacaccatg atcgtcttcc cctgctggcc caatcgccgc agcaccgccc
     6781 gcatctcgat ccgcgcccgc ggatccaggc ccgacagcgg ctcgtccagc agcagcacct
     6841 gcggctcatg gagcagcaca cgggccagcc ccagccgctg tgtctgcccg cggctgagcg
     6901 tgttggcata ggcgtcgcgc ttgaagtcga gatcgacgag ctgcagcttc tcgtcgcaga
     6961 cctgccgccg cttgggaccg ctgatccgat atgccgcggc gaagaactcc aggtactcga
     7021 tcaccttcat gtcgtcgtag acgccgaaga agtcgggcat gtagccgacc agccggcgga
     7081 tttccttcgg atcgcgatag atcgagtggc cgcagacgta ggcctcgccg gaggtcgctc
     7141 ccagcagcgt ggcgaggatc ttcatcgtcg tcgtcttgcc cgcgccgttg gggccgatga
     7201 agccgaacaa atcgccggcc gccagatcca gctcgatggc ccggatcgcg taggactcgc
     7261 cgtatttctt cgtcaggttg tgcgtcttga tcacagcggc caggagtcgg gagtcgggag
     7321 tcggggatca gcgtttggtg tgaggcgaga cgggaaggat caggcggtac caggtccaag
     7381 tggttgtgtc ggcagcggcg gcgagcggtg cagcttcccc agccctagcc cctaacccta
     7441 gccctgctac cggctgtttc gcccggccga ccagcacggc ctggcccagg cgaatgtgct
     7501 ccgaaaggtc gatctgcggc tgatagcggt gcgacagccc ggtgtagctc cgtccgccgg
     7561 cagcttcgtg aaacatcagc atctgcacga tctgcggcac gtcggtcgcc gcctggttcc
     7621 agggcgttgt cacgtctttc gactgttcga cccgccgctc ggtcagccgc cattccagat
     7681 tgagggcccg ctgcggatcg acgaccaccc gcgcgcccgg ctgcagcgtc ccgaggcgaa
     7741 atagcttttc gccgtgcgcc agcaggcaat cgctcagttc aaccggcagc ggctgcgtga
     7801 attcgccacc gaccaggccg aactcgtcga gtgcgagctt gctctcgacg gtcagcggag
     7861 ttttggccca ccactgcgcc gagaggctct tggacgaggc aatcgtcgtc ggtaaatcca
     7921 gggtggccgg cgcgggccct ggcatgttca cgcgataagg ctcgacgctg gccagcacga
     7981 cctgccgcga gccgagcccg ccgagcgcct cgcccggcag cccctgccac gagagccagc
     8041 cgtgtgggct gcccgccgcc gccggaagat gcaggtagac gtcgtaccgg gccgtccgtg
     8101 ggctatagag gtgggcccac acggtgccgc gcacgagccc gcggtccacg tcgaaatcga
     8161 cgatctccac cagattcagc cgcgtccgcg tgccgtgggc ctgccggctg atgtagccga
     8221 acgccagcgt ggcggccagc atcagcagcg ggaacgtgat ccaggtgagc tggcgcggcc
     8281 agccggtgcg agacagcagc aggtagtcgc ccggcccgac gatgagcaga aacacgatgg
     8341 tcagcacggc gacggtcgtg aagttgacga gtgtgacgcc cgagaattgc tccagggcca
     8401 gccgcagctg cccgaccagg tcggaatagc ccaggtggcc gacgccgctg ccgcgctggc
     8461 gctcgtcggc atcgagcgag ctgcggtcgc gctggataag agcggcgatc agccgcgagc
     8521 ggccctccca ggcggccagc gacggatggt cgaggtccag ggcgacgaat gtgacctctc
     8581 ccaggccggc gggggcgtgc acggccagcg gccgcgtggc aagggcgctg gcttcgccca
     8641 gcagcacttc gccgcggacg ttcttcagcc gcgtcaccag cgggcggttc cgctggaaga
     8701 actcggcctg gaagggaagc tcggtgcggg tgaaagtctc caggccggcc cgctcccgca
     8761 gcggcacgac ctcctcgaaa tcgccgggaa cgagcgtttg ccacgcgctg cccgccgccg
     8821 tgatctccgc cccgcgcgcg ccggccgcga gcaccagccg cccgccgagc agcacccagt
     8881 cgatcagcgc ctgccgctgc gcggccgaca tggattccag gaacttcgcg tcgctggtgg
     8941 tgagcaggac ggcatcgacg ccgctatagg cccaccagac gtccggcagt tctgccgccg
     9001 actgcacctg cgccgtgacg agcgtcgctt cagagcggcg tctcagagtg gcggccgcgt
     9061 cgtccagtcc ggcggaaggg ccgataccga cgacgaattc ctgcgtggcg ccgatcgtcg
     9121 gcgtcgccag atcctgcgac cagacgacgc ccgcatccgt ccgcagctgg agcacgagca
     9181 gcgcggcgat agggccgctc ttggcataca gcagcaccgt cgtctcttgc tgcggcgaga
     9241 gattgagctc aggcgcttgc tcgttggcat agatcacggg cgtctggtcg ccgtcggccg
     9301 ccacgacctc caggcggccg cggacggacg tttcgccagc gacgagcgtg gcccgcacgg
     9361 gctgccagaa ccccgacttg aatttgccgc cgaaaccgac gctgctcgct ttcacagtca
     9421 gcaggtcatc tcccgcggcg agaccgaccg gcagcgaagc ggcaaacagg gccgccagca
     9481 aggcggcggg aacgaattgg cggagcgaag acatcagcgg tggtacgaat ctcagacggg
     9541 cgatgttcaa tcgtagagca agcgtcctcg cttgctcacg catactactg acgtagaact
     9601 gcacaagcga ggacgcttat gctacggctt ctcgctgtcg gggcaggtgc agacgaactt
     9661 gccgcagccc gggcagccgc ggccgtattt ctgggcgatg gcctccgaca ggtcaacgcc
     9721 ggcgacgttg gcgatcgtgg ccagccaggc gagcacgtcg gcgaattcct ccagccgctc
     9781 ctggtgcgtg ccgctgcgga gcgcggtggc cagctcgccg acctcctcca tcagccacaa
     9841 gaacgtgcct tcgacgccgc gggcctggtc cttctcaaag tacatcttgc ggatcagggc
     9901 ctgaaggtcg gccagcgaga cttgcggctt ggtgtcgttc actgcgtaag gctcttttcc
     9961 agttcggcga tttgcgggtg cttgggatcc agttcgcgga gtcgctcaat cgccgcgccg
    10021 gcctcttcct ttttgcccag cccaatgagc agcctggcga gcgccgcttg ccaggcaggc
    10081 tgcttgccgt ccagccgcag agccgtctgg tattcgtcca cggcagcggc gttcttttcc
    10141 tgagcggcca gggcggcggc gagcagagcg tgcccggcag cgtactcgac gtcgcgatga
    10201 atgatctcca gcgccgcgcc agccgctgcg ccgtagtcct tctgcgccag cgagacttcc
    10261 gcgagcttct ggcgaatggt caggttgtcg ggctcagcct ggctccaacg gcgaaggacc
    10321 ggcagcaatt tggcctccgc atccggcagc ccgcccggct gcaaatagac gcgggccagt
    10381 cccgttaccc agcggtcgct gccagggaat tgttgatcgc caagctgata cagttcttcg
    10441 gccgtcttga agtcgccgtc cttcagcttt actgcggcga gcaaggccaa ggcttcttcg
    10501 tgtagtgcgg acttgtcgag gccgttttcc agcagcttaa ccgctgcgtc gaaatcgccg
    10561 atcgacatct gctgccgggc gagcacataa gccgcgcccc cgtgccgggg cttttgcttc
    10621 tgcgcctcga gagcccactt gcgggcgtcg gccgccagcc cgcgatcgag acacgccaga
    10681 gcgaactccg cggcgaggtc ggcatcgggc gggcccgcct cgacctttcg ctccaggtcg
    10741 gcgaacttgg gcttctcccg cgggattagt ttgccggcgc cctcgatctg cttcgaaaca
    10801 tactccttgt attttgcctc caggtcagct tgctttacgc cgtagcaccg cgcgagggca
    10861 tcggcggtcg tcagccggtc gcggtacgcc gcgaggagtt tggcgatcgc gtcctctccg
    10921 tgggttgcca gcaggaactc gacgtacaac tccgcctggc aataggccag cgtccagtcg
    10981 tcgcttgatt gcggccggat gaagcccagc gtcagcgtgt ccagatcgaa cagctcgccg
    11041 gacctggctc ggctggccag cagctgagtc cacttcttcg gccgcggctg tgcctccaag
    11101 tgaacggcca gcccctcggt cagccagtgc gggacgccga agtcggtctg ctgcaggttg
    11161 agcacgtgca cgaactcgtg ccgcaggacg gtggcccagt cgtatttctc cggcagctcg
    11221 ccgggcgaag tgagcgccac catcttgccc gcgcaagccc cgactgtgtg gatgaacggc
    11281 agcccgacca tccgggcgct gaaccagctg tggccgctgg tgcggccgct cctattgaag
    11341 atttcgatca gcgtcttgcc ctcgggcgtg tagcccagca gcctggtcag ttgcgggaag
    11401 acttcctcct ccaagtgccg cgatgcgtaa cgggccagca gctcgtcttg cccacggtcg
    11461 aagcgaatca caaagtgctc ggtctcgatc gtggcgtagt ccttcaggac gtcgagcacc
    11521 tcgagcatgt tcttgacgcg gacgttgaac gggtcgatgg caaaggactc gttcagcagc
    11581 ttcgccgctt cggcctcttc acccaggcgc atcagcgtga ggccgagttc cccgcgggcc
    11641 gagatcagct gcgggagctt gtcgttggcc aggcggtagt attcggcagc ccgcgtgaat
    11701 cgccgcatcc ggtcgaaact ctcggcggcg gccaggtaaa cctcgccgca gtgtgggttg
    11761 ttccgggttg cgccactggc aatttgctcg actttgtgcg gcgggccggc gatcgtgtga
    11821 gccgccagca gccggccgag cgtcgcctcg tcgcgcggat tgagctggag tgctttttcg
    11881 aggattggaa tcgcctccgc cggccgcaag tctgccagca ggctgtcggc ccgcagctgg
    11941 tgggcccaga tcagctcggg gttgatctcc agcgcccggt cgagcgacga tttggccgcc
    12001 gccaggttga accgcgacag ggccagcgcc gccttggccg cgtgcagttc ggcggcgtgc
    12061 ggattgatcg ccaggccgcg gtcgatctcg gcggtcgctt ccgcctcgtt gtacttttcc
    12121 agatacaaga gcgccgcctc gaagtgcgcc ggccagaagt cggacgcgcg ccgcagggcc
    12181 gacggataga cgtcgttcac caggcggcgg aactggttgc tgttccgcgt ccagcgggcg
    12241 tgctcggcga ccgcccggcc gatcagcagc aggtccatcg gatcggcgat ctgcggggcg
    12301 cggttgtggt agccgatgaa ccaggcatag gctgactggg cctcgctgat cttgcccgac
    12361 agccgcagca gctcggcctg gacccagtga gcgctcacgc agtctttgtc gagcgcgacc
    12421 gcggcggcgg cctccctggc agccgcgtcg tgatcgccgc gccggagcgc caaaagcgcc
    12481 agctcgccgt ggatcgccgc cgacttgggc aatttctcag ccgcggccgt cagcccggcg
    12541 gccgcgtcgt cgcgcctgcc ggtggctagt cggcagcggg ccaggccaat tgcggctgct
    12601 gggtcctcgc cggcactctt ggcgaactcc gcgacggcct catcgtagcg gcccttcagc
    12661 agcagcgtcc gcggcgggag cgaatcatcc gcagccgcga tcgtgggcgc aaacagcgca
    12721 gcacaaacga atatggcgac gcccacgcgc atcggaagcc ctgtttgagt ctgcggccca
    12781 ggcctacaat tcaatccact cgagtttctt gatcgtaacc caaaggtctg gcagcggaca
    12841 tgacggagtt tgtgacggtc gccaaagtcg acgaaatccc ggcgggggaa gggcgggcct
    12901 tcgccgtgaa cgggcggatg gttgccgtct tcaacgacga ggggcgatat ttctcgatcg
    12961 acgacttctg cccgcacatg ggcgcgtcgc tggccggcgg ctggctggaa aaggggaccg
    13021 tcacctgccc ctggcacgcc tggcggttct gcatccacga cgggaagtgg ctcgacaacc
    13081 ccaaggtcgg cgtcgatgcc ttcgaggtcc gcctcgccgg cgacgaaatc caggtcaaag
    13141 tcccgcccaa gcaagtcccg ccgctgcccg ggacttgacc tgccgccagg tcagcgggcc
    13201 ttatgctgca tatgcagacc attttttcgg acgggttctc ggaacgcaac tgcttgctgc
    13261 caatgcattt acgccaagaa atgctgcata gcgttatgca cgttatgcat aattaggcca
    13321 ggttgcggcg gactgtatgc agctttagcc ggacgcctga ggacgccccg cgttcattca
    13381 accagcttgt caatgagcgt cgctcgccgc aggagtggcg aggatcggcg ctattgtaac
    13441 tggacgtaca cttagagcaa gagtgtggca aagtcgttgc tcatgccgcc gagctgagca
    13501 gggtctggca agtgtgtcat gcacccaagt tcgcatgatc gcggctaaca acaactggcg
    13561 tgtcgccgtt tggttagcta gattccgtgg cgaccagtcc ttggcggagc ggaactgcga
    13621 gctaaccatg aattcactga aatcggccac aggtctgacg ttcattctta ttgtcaccgg
    13681 ctccctctgg gggcaggctc ctcgagcggc agaaaaccag ttgaacgcgg gtcgccaaac
    13741 cggctggcag cttgtgcaac tggtcgtgga aaaactggac gccgggcccg gcggcgagtt
    13801 tccgggcatc gcggcttgga agcaggattt cgccacggcc acgcgaggca tcgatgcggc
    13861 agctcctgcg gacaaatggc ccgccgtggc tatggacgc
//
LOCUS       DASRJB010000879        12818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2229085, whole genome shotgun sequence.
ACCESSION   DASRJB010000879 DASRJB010000000
VERSION     DASRJB010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12818
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229085"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="atpA"
                     /locus_tag="VFB80_23105"
     CDS             <1..1127
                     /gene="atpA"
                     /locus_tag="VFB80_23105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZN36745.1"
                     /translation="LAPGVAGRQPVREPLQTGIKAIDAMTPIGRGQRELIIGDRKTGK
                     TTIAIDTIINQKNSGVKCFYVAIGQKESSVASTIRVLQERGAMDYTTVILAGASTPAP
                     LQYVSAYAGTSMAEYFTYKGQHALIVYDDLSKQAVAYRELSLLMRRPPGREAFPGDVF
                     YCHSRLLERSCKLSKALGGGSLTSLPIIETLEGEVSAYIPTNVISITDGQIYLQPDLF
                     FKGVRPAMNVGISVSRVGGSAQIKAMKDKQVAGGLRLTLAAFRELEAFAQLGTDLDAA
                     TQSRLDRGYRMVELLKQPQYQPLDVVDEVMIVFAGNSGSLDDVPINRVQEFEKEFLQF
                     MKDSKAAIRNEIAEKRELTPELKQALTEAIQQFKASFKSK"
     gene            1159..1539
                     /locus_tag="VFB80_23110"
     CDS             1159..1539
                     /locus_tag="VFB80_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HZN36746.1"
                     /translation="MSIPDKPPPEGSIPTPEERQWALLAHLAGLIATAMGGLSFLGPL
                     IVWLIKKDQSPFVADQAKEALNFQIAVTIALLVSVGIALATCIGAILLPIVGIGSLVF
                     AIIAAMEANKGVYYRYPYTIRLIN"
     gene            1612..2499
                     /gene="atpG"
                     /locus_tag="VFB80_23115"
     CDS             1612..2499
                     /gene="atpG"
                     /locus_tag="VFB80_23115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZN36747.1"
                     /translation="MAKARALDKRRKSIRNIRKITRTMELIATARYKKAMDRATAATA
                     YTRRITQVVADLAQAGLEVSHPLLQPHDEVKHATLLVLTANRGLCGGYNGNVMRTSLP
                     RLEELRKLVPRVSIEVSGKRGVATCKFRKVQLAHSYTQFDDQPKFEQVEAIATRYLEE
                     YSTGQLDRLDVAYTKFLSSAKQSAVVETLLPLGQLEGADAKKEEENRGESMYEFLPSA
                     ESILQEVVPASFKAKLFKCFLDAAVSEQIARMVAMKSATENASEMIKELSSRYNRARQ
                     GQITGEIMEIIGGVEALKG"
     gene            2580..3251
                     /locus_tag="VFB80_23120"
     CDS             2580..3251
                     /locus_tag="VFB80_23120"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN36748.1"
                     /translation="MIASSTALLGSPRAPLLGDPTYPTRDGRPMGETDLHRRIMFDLI
                     NMLERHYAGQEVYVSGNLLLCYEQGNRRKHVSPDVLVTKGLRPGLRLNYILWQERLPP
                     NVVIEVTSKTTRKEDIETKFEIYQNVIRVAEYFLFDPYGDYLDPPLRGHRLKRGQYVP
                     INLSRGRMHCEQLGIDLVPEYQALALVDRKTGQRLKTADEVAEEQAEEIARLQSEIKG
                     LRKGK"
     gene            3303..4757
                     /gene="atpD"
                     /locus_tag="VFB80_23125"
     CDS             3303..4757
                     /gene="atpD"
                     /locus_tag="VFB80_23125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZN36749.1"
                     /translation="MATATANGNIGKITQVIGSTFDAEFEEEHLPAIYNAVKIDSEKK
                     GVKINLTGEVQQHLGGGRVRCVALGSTDGLIRGQDVLDTGAPVSVPVGEGTLGRVFNL
                     LGEPIDDRGPVTTKERWPIHRQSPALADLSTNTEIFETGIKVIDLLTPFVRGGKAGLF
                     GGAGLGKTVILQELIARVASSHGGFSVFAGVGERTREGTDLWIEMQEAEIGNTGRRVI
                     EQTCMVFGQMNEPPGARLRVALSALTMAEYFRDLTGKDTLLFVDNIFRFSQAGSEVSA
                     LLGRMPSAVGYQPTLATEMGALQERIASTKKGAITSVQAVYVPADDPTDPAPATAFGQ
                     LDAFLYLERSISEKGIYPAVDPLASSSRILDPQYVGPRHYAVARRVQGTLQRYRELQD
                     IIAILGVDELSEDDKLVVHRARRIERFLSQPFLVAEVFTGKPGEITKIEDTIRSFDEI
                     CDGKWDHLPEDAFMYVGPIEQAEEQAKRLAKGGK"
     gene            4767..5168
                     /gene="atpC"
                     /locus_tag="VFB80_23130"
     CDS             4767..5168
                     /gene="atpC"
                     /locus_tag="VFB80_23130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZN36750.1"
                     /translation="MPELTCTVVTPEQTALETKAEFVALPLFDGEIGIAPNHSPLIGR
                     LGYGELRIKSGGQTVAYYIDGGFVQVADNVVAVLTNRAVPVESLDAAAAEAALKTALE
                     KPANTPELMAIRDRAVAQARAQIRAAAHARR"
     gene            5309..5932
                     /locus_tag="VFB80_23135"
     CDS             5309..5932
                     /locus_tag="VFB80_23135"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN36751.1"
                     /translation="MDVPIYRLDQYSLPQPQSLPGQALVELEITRGRVQQRLRPVHSK
                     IFLIGAAHDCDLVLGDLQFPEAYAYLFVSGCEVSIRRLGAGPELTVCGQAVEGAELFH
                     GDRIAFGPFELRVRIESAAGGLPDNDGYLPQAESGLMARDRRAAVDEVESLLLDIRRG
                     LADDPPVASPPIRPLATSGLPPLPRSLASPNSSASSRAYREPHVARS"
     gene            5916..6194
                     /locus_tag="VFB80_23140"
     CDS             5916..6194
                     /locus_tag="VFB80_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36752.1"
                     /translation="MSLAPSPVIGTICIEPLATREACPCDDSTLGLVLESFAHLDSLA
                     EEPVRLVASTRLSRLAALGPALGVRANLAQLLADLQAEYEQVTERLES"
     gene            complement(6910..8550)
                     /locus_tag="VFB80_23145"
     CDS             complement(6910..8550)
                     /locus_tag="VFB80_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN36753.1"
                     /translation="MRRLNLIAGAAIALAGLGLLAFTFHMLVNCIYIYEGESLRLRYK
                     GPLVFGTGKTAEAGQYAQPGEIGVYEEMKGPGRYMWYCPIWWERVRVPDVVVKPGEVA
                     LVTSKMGDSLPPGQFLVDGELTGPNRAQHKGTLRKVLGPGRYRINNYGFESKIEKLVQ
                     TKVGDNLKHAGWVNIPTGYVGVVTNLAANSASGQKGGIQDQVLPPGIYAVNPAERQID
                     IVGVGFWESSISTDHKRGPDGKDLLDASGEPEAVAGSGIAFPSNDGFNIQLDFTAIWG
                     VMPKDAPKVVETFGTVSAAEQKVIIPQSESISRINGSKMGANALLVGETRQAFQNSVS
                     DHFHEVLKDKKLTLLYGLVRHIYIPKDIRTPLQEGYVADELKLTREEERTTKQAEGEL
                     REAEKKVVQETEKVKVETAKLVASALAEGQKKVGEIEAAAKQQVAAIDRQIAEFDAKK
                     IEMLGKAKSEADRLLKEANSQKFDLAVKAFGNAGAYTRWQFAEGLPENIQLQLFYAGE
                     GTLWTDLKGMMPTLPLTNPAAPRPAETPAAPRPVSAGK"
     gene            complement(8581..10182)
                     /locus_tag="VFB80_23150"
     CDS             complement(8581..10182)
                     /locus_tag="VFB80_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN36754.1"
                     /translation="MVRQRESFATDGNSVPWVFFFGGLLVLASIVGAPIFYYGLCQFN
                     VPTGYMAVLTRKTGRDIANGDELAPDATYKGLQKEVLAEGRHFRNPWTWDWEVVPQVE
                     IPPGKTGVRIRLHGDDPPGGETIAWRENEKGIVPGVLRPGRYPINAVVSGQTRQRDNY
                     AEIIELHDMVTVPAGFRGVVTNLAGPMPKDPNTIVLLPSDQGKRGVESQTLDAETYPE
                     LSNPYIHRVQLVDCRSQRFNLSQDGDMGFPSKDGFWVVLDGIIEFRVMPEKAAEVYVI
                     YNQLENDSNGTRIDEEVIKKVVLPNARSFCRLRGSDHSGREFISGDTRTKFQEDFQKE
                     LEKNCESQGIEIIQALITKISPPQQIAQPVRDRQIAVEKKKQFGRQLLQQESEKQLAI
                     ETEMNQRLQALVGAQQQVIKITTEASQAQEIAVIEANKRLKVAELDLQAATDLAAATL
                     ARGTADAKVVEFQNAAEAAGWQKAVQAFGGSGDEYARWTLLKKLAPAYRSIMVNTHDS
                     PIMDIFRQYEQQSAPPKPVKTAGGN"
     gene            10346..>12818
                     /locus_tag="VFB80_23155"
     CDS             10346..>12818
                     /locus_tag="VFB80_23155"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZN36755.1"
                     /translation="MKSARWATLLAVAIPIAVLLGPLLVTDRSFAMRDAAHFYYPLFA
                     WCARQWSAGSVPLWNPYENCGLPVLADASSSIFYPGKLLFLLPVNFALRYKLYVVLHV
                     VLAAAGSYALARHWKASPQAAALAAIAYSCGGNVVFQYCNVVFLVGAAWLPLAALAAD
                     RMLAGRSWLSAVLLGLALALMILGGDPQAAYHSLLITALYAVVIALARGDATHSATDR
                     FRRLGLGIGLTGTAAIVGFLLAAVQVLPSSEATQYSERAAVDRPRNIYEALTTAAPAG
                     ESRPQAIAKGFFGKPDRGTHQDLAYDFSVGPWRLVEYLWPNVAGRMLPTNRRWFSLLP
                     MEGRTWTPTLYLGLVPLLLGLAGFRLWGRAEPRQRWLSWLVLIFTLGSFGYYGLGWLA
                     TEAYATLFRGDAAKIDLAPGVGGVYWFFVTFLPTYVYFRYPAKLLPLVSLGLSQLAAL
                     GWDRMHAQPQRRLTAVLLILGILSGILAFIIWSIDPTFISALVRGRGDSSLGPFDAAG
                     ARRDVLFAFLQAAVVALWARWLLLKSWNSPTQQARWQWSLLVLTAIEVAVANAWLVVA
                     APASVWRDPSPVAAAIQAASDESDLPPRVFRANLGGWRPPSFRQKSSAERPAESAAWE
                     KDTLFPKYHLPSRLSLVESYGSIKLLDYESLLFVARASGPKQPDGSLLPHPAALRLVG
                     TEFLLLPASHEPKFAEPMQPAAGTRWPEDAVLWRMARTLPRAWVVHDIAVLPPLPRPP
                     RLAAIDQRSSEVLFPDRKSRDFLRTAVVETDQPLAEWNAPPAESPTTAEPCRITHYDP
                     QRVVVEVELARPGLLVLGEAWYPGWQ"
BASE COUNT         2161 a         4193 c         4082 g         2382 t
ORIGIN      
        1 tcctcgctcc gggcgtcgcc ggccggcagc ccgtgaggga gccgctgcag acgggcatca
       61 aggcgatcga cgccatgacg ccgatcggcc gcggccagcg ggagctgatc atcggcgacc
      121 gcaaaacggg caagacgacg atcgccatcg atacgatcat caaccagaaa aactccggcg
      181 taaagtgctt ttacgtcgcc atcgggcaaa aggagtcgtc cgtcgccagc acgatcaggg
      241 tgcttcaaga gcgcggcgcg atggactata cgacggtgat cctggccggg gccagcactc
      301 ccgcgccgct gcagtacgtt tcggcctatg ccggcacgtc gatggctgaa tatttcacct
      361 acaaaggcca gcacgcgctc atcgtctatg acgacttgtc gaagcaggcc gttgcctatc
      421 gcgaactgtc gctgctgatg cggcgtccgc ccggccgtga ggcgtttccc ggcgacgtgt
      481 tctactgcca tagccggctc ttggagcgct cctgcaagct cagcaaggcg ctcggcggcg
      541 gctcgctgac cagcctgccg atcatcgaga cgctggaagg cgaagtctcg gcctacatcc
      601 cgaccaacgt gatctcgatc accgacggcc agatctattt gcagcccgac ttgttcttca
      661 agggcgtccg cccggcgatg aacgtgggca tctcggtcag ccgcgtcggc ggctcggccc
      721 agatcaaggc catgaaggac aagcaggtgg ctggcggcct gcgtctgacc ctggccgcgt
      781 tccgcgagct ggaggccttc gcccagctcg gcaccgatct cgatgccgcc acgcagtccc
      841 gcctcgaccg cggctaccgg atggtcgagc tgctcaagca gccgcaatac cagccgctgg
      901 acgttgtgga cgaggtgatg atcgtgttcg ccggcaacag cggctcgctc gacgacgtgc
      961 ccatcaaccg cgtgcaggag ttcgagaagg aattcctgca gttcatgaag gacagcaaag
     1021 cggccatccg caacgaaatc gccgagaagc gggagctgac gccggagctg aagcaggccc
     1081 tgacggaggc gatccagcaa ttcaaggcga gcttcaagtc caagtgagtg gtgagtggta
     1141 agtggtgagg ccgcagcgat gagcattccc gacaagccgc cgccggaagg atcgattccc
     1201 acgcctgaag aaaggcaatg ggcgctgctc gcgcatttgg cgggcctgat tgccactgcc
     1261 atgggcgggt tgtcgtttct gggtccgctc atcgtgtggc tgatcaagaa agaccagtcc
     1321 cccttcgtcg ccgatcaggc gaaagaagcg ctcaatttcc agatcgccgt gaccattgcc
     1381 ctgctggtgt ccgtgggaat tgctttggcg acctgcatcg gtgcgatcct gctgccgatc
     1441 gtgggcatcg gctctttggt gttcgccatc atcgccgcga tggaagccaa caaaggcgtt
     1501 tattaccggt atccgtacac gattcgcttg atcaattgat gtagggtggg ctgtgcccac
     1561 ccaagatgaa ctggtgggca cagcccaccc tactgaagcc aaccgcgtcc catggccaaa
     1621 gcccgggccc tcgataaacg ccgcaagtcg atccgcaaca tccgcaagat cacgcggacg
     1681 atggagctga tcgccaccgc gcgctacaag aaggcgatgg accgcgcgac cgccgccacg
     1741 gcctatacgc ggcggatcac gcaggtcgtt gccgacctgg cccaggccgg actggaggtc
     1801 agccatccgc tgctccagcc gcacgacgag gtcaagcacg ccacgctgct tgtcctcacg
     1861 gcgaatcgcg gactctgcgg cggctacaac ggcaacgtga tgcggacctc cctgccccgc
     1921 ctggaagagc tgcggaagct cgtgccgcgc gtatcgatcg aagtcagcgg caagcggggc
     1981 gtcgctacct gcaagttccg caaggtccag ctcgcgcaca gctacacgca gttcgacgac
     2041 cagccgaagt ttgagcaggt cgaggcgatc gccacgcgct atctcgagga gtactcgacg
     2101 ggccagctcg atcggctgga cgtggcctac acgaagttcc tcagcagtgc caagcagtcg
     2161 gccgtggtcg agacgctgtt gccgctgggg cagctcgaag gcgccgacgc gaagaaagaa
     2221 gaggaaaacc gcggcgagtc gatgtacgag ttcctgccgt cggccgagag catcctgcag
     2281 gaagtcgtgc cggccagctt caaggcgaaa ctcttcaagt gcttcctcga cgccgcggtc
     2341 agcgagcaga tcgcgcggat ggtcgccatg aagagcgcca ccgagaacgc cagcgaaatg
     2401 atcaaggagc tgtcgagtag atacaaccgg gcccgccagg gccagatcac cggcgagatc
     2461 atggagatca tcggcggcgt cgaggcgctc aagggctagt tcaaggtgca aagttcaaag
     2521 ttggtagaat gggaaaaggg acgttcgcag aacgtccggc agtttgtcgg gttgcgtaca
     2581 tgatcgcttc atccacagcc ttgcttggtt cgccgagagc cccgctgctc ggcgacccga
     2641 cgtatccgac gcgagatggc cgcccgatgg gtgaaacgga cctgcatcgc cggatcatgt
     2701 tcgacctgat caacatgctc gagcggcatt acgcgggcca ggaggtttac gtctccggca
     2761 acctgctctt gtgttacgag cagggcaacc gccgcaagca cgtctcgccc gacgtgctgg
     2821 tcaccaaggg cctgcggccg ggcttgcgac tcaactacat tctgtggcaa gagagactgc
     2881 cgccaaacgt cgtcatcgag gtcaccagca agacgacgcg caaggaagac atcgagacga
     2941 agttcgaaat ctatcagaat gtcattcgtg tcgctgagta tttcctgttc gatccctacg
     3001 gcgactatct cgatcctccg ctgcgcggcc atcggctcaa acgcggacaa tatgttccga
     3061 tcaatctgtc gcgggggcga atgcattgcg agcaactcgg aatcgacctc gtgccggagt
     3121 atcaggcgct ggccctggtc gatcgcaaaa ccggccaacg cttgaagacc gctgacgaag
     3181 tcgccgaaga gcaggccgag gagattgccc gcttgcaaag cgagattaag ggtctgcgaa
     3241 aaggcaagtg aagctaccat ttccatgcgc aaatttgcac tcaacacgcg agtaggcttt
     3301 tgatggcaac ggcaactgca aacggcaaca ttggcaagat cacacaggtg atcggctcca
     3361 cgttcgatgc ggagttcgag gaggagcatc tgccggcgat ttacaacgcc gtgaagatcg
     3421 actccgagaa aaagggcgtg aagatcaacc tcaccggcga ggtgcagcag cacctgggcg
     3481 gcggccgcgt gcggtgcgtc gcgctgggca gcacggacgg gctgatccgc ggccaggacg
     3541 tgctcgacac cggcgcgccg gtctccgtgc cggtcggcga aggcacgctg ggccgcgtgt
     3601 tcaatctgct cggcgaaccg atcgacgacc gcggcccggt cacgaccaag gagcgctggc
     3661 cgatccatcg ccagtcgccg gcactggccg atctctcgac gaacaccgaa atcttcgaga
     3721 cgggcatcaa ggtcatcgac ctgctcacgc cgttcgtccg cggcggcaag gccggcctgt
     3781 tcggcggcgc aggcctgggc aagacggtca ttttgcaaga gctgatcgcc cgcgtggcca
     3841 gctcgcacgg cggtttctcg gtgtttgccg gtgtcggcga gcggacccgc gagggaacgg
     3901 acctgtggat cgaaatgcag gaagccgaga tcggcaacac aggccgcagg gtcatcgagc
     3961 agacctgcat ggtcttcggc cagatgaacg agccgccggg cgcgcgcctg cgggtcgccc
     4021 tgtcggcgct caccatggcc gagtacttcc gcgacctgac cggcaaggac acgctgctgt
     4081 tcgtcgataa catcttccgt ttcagccagg ccggcagcga agtgtcggcc ctcctcggcc
     4141 gcatgcccag cgcggtcggt tatcagccga cgctggccac ggagatgggc gcgctccagg
     4201 agcggatcgc ctccaccaag aaaggcgcga tcacctccgt gcaggccgtg tatgtgccgg
     4261 ccgacgatcc gaccgacccg gcgccggcca cggccttcgg ccagctcgac gccttccttt
     4321 acctggagcg gtcgatttcc gaaaaaggca tctatccggc cgtcgatcct ctggcctcct
     4381 ccagccgcat tctcgatccg cagtacgtcg gcccgcggca ctacgccgtc gcccgccgcg
     4441 tgcagggcac gctgcagcga tatcgcgaat tgcaggacat catcgcgatc ctcggcgtcg
     4501 atgagctgag cgaagacgac aagctggtcg tacaccgggc ccgccggatc gagcggttcc
     4561 tctcgcagcc gtttctcgtc gccgaagtct tcaccggcaa acccggcgaa atcaccaaga
     4621 tcgaagacac gatccgcagc ttcgacgaaa tctgcgacgg caagtgggac cacctgcccg
     4681 aggacgcctt catgtacgtc ggcccgatcg aacaggccga ggaacaagcc aagcgcctgg
     4741 cgaaaggcgg caagtagcaa ccctccatgc ccgagctcac ctgcaccgtc gtcacgcccg
     4801 aacagaccgc tttagaaacc aaggcggagt ttgtcgccct gccgctgttt gacggcgaaa
     4861 tcggcatcgc gcccaatcac agcccgttga ttggtcgcct gggctacggc gagctgcgga
     4921 tcaagtccgg cggccagacc gtggcgtatt acatcgacgg cggtttcgtt caggtggccg
     4981 acaatgtcgt ggccgtgctc accaatcgtg ccgtgccggt cgaaagcctc gacgccgccg
     5041 ccgccgaagc ggcgctcaaa accgcgctcg aaaagccggc caacacgccc gagctgatgg
     5101 cgattcgcga ccgcgccgtc gctcaggccc gggcccagat tcgcgcggcg gcgcacgcgc
     5161 ggcgatagct ctgccgcctg taacgcggcg ccgcccgttt cacgtgctgc tttctccgcg
     5221 gattcgcgcg ccgcggctta ctcccctctt gcgccgcggc cgatgatttc tttagccggc
     5281 agctctttgc cgcgggaaac tggctcccat ggatgttccg atttaccgtc tcgatcagta
     5341 cagcttgcct cagccgcaga gtctccccgg ccaggccctg gtcgagctgg agatcacccg
     5401 cggccgggtc cagcagcgcc tccgccccgt ccacagcaag attttcctga tcggcgcggc
     5461 ccacgactgc gacctggtgc tcggcgacct ccagttcccg gaggcctacg cctacttgtt
     5521 cgtcagcggc tgcgaggtct cgatccgccg gttgggcgca ggcccggagc tgaccgtctg
     5581 cggccaggcg gtggaagggg ccgagctgtt ccacggcgat cggattgcct tcggaccgtt
     5641 cgagctgcgc gtgcggattg agagtgcggc cggcggcttg ccggacaatg acggctactt
     5701 gccgcaggcg gaatcggggc tgatggcccg cgatcggcgg gcagcggtcg atgaagtcga
     5761 aagcctgctg ctcgacattc gccgcgggtt ggccgacgat ccgcccgttg cctcgccgcc
     5821 gattcgtccg ctcgctacgt ctggcttgcc ccccctgccc cgttcgctgg ccagtccgaa
     5881 ttcctccgct tcctcccgcg cctatcgaga accgcatgtc gctcgctcct agcccggtga
     5941 ttggaacgat ctgcatcgag ccgctggcca cgcgcgaagc gtgcccctgc gacgactcga
     6001 ccctcggcct ggtgctggag agcttcgccc acctcgactc gctcgcggag gagccggtgc
     6061 gtctggtcgc cagcacgcgg ctgagccggc tggccgcgct ggggccggcg ctgggcgtcc
     6121 gcgcgaatct cgcgcagctc ctggctgacc tgcaggcgga atacgagcag gtaaccgagc
     6181 ggctggaaag ctgacacgcg attcatgacc acgaaaaacg cctcgtgtta aagaatccgg
     6241 ccctggctcg aagtcgccga cacggcaagc ccctgcggcg aaggacctta ggccgatctt
     6301 gccatgctct tgaacttccg aattcctgac caaaacggcg aagcttgggg gtttcgttca
     6361 aaaaatcgga ccgacctccg ccgcgcccgc attcttcacc gggttgggcg gggcggctcc
     6421 gtggtgaatt aatcggccgc gtcgcgagtg cacccattgg cgtaaactcc attttccacg
     6481 ccacttccag cggcggcctc gcgctgacgg tcgccgaata attcaccaca ggggcgcgaa
     6541 acctgaggat ctggtgaata attcgcaccc cgcgtgataa ctccctgccg cgactggctt
     6601 tgaatttttc accgcggtga agaattcgcc gcggcacgag tgagccggcg cggccgcact
     6661 gcggcgcttg gggcgggccg ggccactcga ttcgccgact ctatccacac gtggacataa
     6721 ttatccacat cggctggcgc tcgtcaagac acttgtgcat atttgttcaa aagagcgcaa
     6781 cttgcgccga tccggggtgg cgcaagttca atccccgcac ggacttccgc aaaccggcct
     6841 gagcgcaact tttttcgcgg gcgaaaacga aatggactag tctggtccat tgggcaactg
     6901 ggcgaagcgt cacttgccgg cgctgacggg ccgcggcgca gcgggcgttt ctgccgggcg
     6961 cggggcggcg gggttcgtga gcggcagcgt gggcatcatg cccttgaggt cggtccagag
     7021 cgtgccttcg ccagcgtaga acagctgcag ctgaatgttc tctggcaatc cctcggcgaa
     7081 ttgccaccgc gtgtaggcgc cggcgttgcc gaaggccttt acggccaggt cgaacttctg
     7141 cgagttggcc tccttgagca gccgatcggc ttccgatttg gccttgccga gcatctcgat
     7201 tttcttggcg tcgaactcgg cgatttgccg gtcgatggcg gcgacctgct gcttggcggc
     7261 cgcttcgatc tcgccgacct tcttctggcc ctcggccagc gccgaggcaa ccagcttggc
     7321 cgtctccacc ttgaccttct cggtctcctg cacgaccttt ttttccgcct cgcgcagctc
     7381 gccttcggcc tgcttcgtgg tgcgctcctc ttcgcgggtt agcttcagct cgtcggcgac
     7441 atagccctct tgcagcggcg tgcggatgtc cttgggaatg tagatgtgcc gcaccaggcc
     7501 gtagaggagc gtcaatttct tgtccttcag gacttcatga aaatggtcgg agacggagtt
     7561 ctgaaacgct tgccgcgtct cgcccaccag cagcgcgttg gcgcccatct tggagccgtt
     7621 gatgcggctg atgctctcgg actgcgggat gatcaccttc tgctcggcgg ccgagaccgt
     7681 gccgaaggtc tcgacgacct tgggggcgtc cttgggcatc acgccccaga tcgcggtgaa
     7741 gtcgagctga atgttgaatc cgtcgttgga cggaaatgcg atccccgagc cggcaaccgc
     7801 ctccggctcg ccgctggcgt cgagcaggtc cttgccgtcg gggccccgct tgtgatcggt
     7861 cgagatgctc gattcccaga agccgacgcc gacgatgtcg atttgccgct cggcgggatt
     7921 gacggcataa atgccgggcg gcaggacctg gtcttgaatg ccgcccttct ggcctgaggc
     7981 gctgttggcg gcgaggttgg tcaccacgcc gacgtagccg gtgggaatgt tgacccagcc
     8041 ggcatgtttc aaattgtctc cgaccttcgt ctgcacgagt ttttcgatct tggattcaaa
     8101 cccgtagttg ttgatccggt agcggccggg gccgagcacc ttgcggagcg tccccttgtg
     8161 ctgggcacga ttcggccccg tcaattcgcc gtccaccagg aactggcccg gcggcagcga
     8221 atcgcccatc ttgctcgtga cgagcgccac ttcaccgggc ttcaccacga cgtcgggaac
     8281 gcgcacccgc tcccaccaga tcggacagta ccacatgtat cgccccgggc ccttcatttc
     8341 ctcgtacacg ccgatttcgc ccggctgcgc atattgtccg gcttccgcgg tcttgcccgt
     8401 gccgaaaacc agcggcccct tgtatcgcag ccgcaggctc tcgccttcgt agatgtaaat
     8461 gcagttcacc agcatgtgga aggtgaacgc caacaggccc aggccggcca gggcgatcgc
     8521 ggcgccggca atcaggttaa gacgacgcat gattcggtcc ccaaagtgat tgtgcgatgg
     8581 ttagtttccg ccggcggtct tgaccggctt gggcggagcg ctttgttgtt cgtactggcg
     8641 gaagatatcc atgatcggac tgtcgtgtgt gttgaccatg atgctgcgat aggcgggggc
     8701 cagcttcttg agcagcgtcc agcgggcgta ttcgtcgccg cttccgccga acgcctgcac
     8761 ggctttctgc cagccggccg cctctgcggc gttctggaac tccacgacct tggcgtcggc
     8821 cgtgccgcgg gccagcgtcg ccgccgccag gtccgtcgcg gcctgcaagt ccagctcggc
     8881 gaccttcagc cgcttgttgg cttcgatgac ggcgatttcc tgggcctggc tggcctcggt
     8941 ggtgatcttg atgacctgct gctgggcccc gaccagggcc tgcagccgct ggttcatctc
     9001 ggtctcgatg gccagctgct tctccgattc ctgctggagc agctggcggc cgaactgctt
     9061 cttcttctcg accgcgattt gccggtcgcg gaccggctgg gcgatctgtt gcggcgggct
     9121 gattttggtg atcagggcct ggatgatctc gatgccctgc gattcgcagt tcttctccag
     9181 ctccttctgg aaatcttcct ggaacttcgt gcgcgtatcg cccgagatga actcgcgccc
     9241 cgaatggtcg gagccgcgga gccggcagaa cgagcgggcg ttgggcagca cgaccttctt
     9301 aatgacttcc tcgtcgatcc gcgtgccatt ggagtcattt tcgagctggt tgtagatcac
     9361 atagacttcc gcggccttct cgggcatcac gcggaactcg atgatcccgt cgagcacgac
     9421 ccagaagccg tccttcgacg ggaagcccat gtcgccgtcc tgcgaaagat tgaaccgctg
     9481 gctgcggcaa tcgacgagct gcacgcgatg gatatagggg ttggagagct cgggataggt
     9541 ctcggcgtca agcgtctgcg attcgacgcc ccgcttgccc tggtcggacg gcaggagcac
     9601 gatcgtattg gggtccttgg gcatcgggcc ggcgaggttc gtcacgacgc cgcgaaagcc
     9661 cgcgggcacc gtgaccatgt cgtgcagctc gatgatctcg gcatagttgt cgcgctgccg
     9721 cgtttgaccg gagacgacgg cgttgatcgg gtagcggccg ggacgaagca cgcccggaac
     9781 gattcccttc tcgttctcgc gccaggcgat ggtctcgccg cccggcggat cgtcgccgtg
     9841 cagccgaatc cgcacgccgg tcttgcccgg cggaatctcg acctgcggca cgacttccca
     9901 atcccacgtc cacggattgc ggaagtgccg gccttcggcg agaacttcct tctgcaggcc
     9961 cttgtaagtc gcgtccggcg cgagctcgtc gccgttggcg atgtcccggc cggtcttccg
    10021 cgtcagcacg gccatgtagc cggtgggcac attgaactgg cacaggccgt aatagaagat
    10081 cggcgcgccg acgattgacg ccagcacgag cagcccgccg aagaagaaga cccacggcac
    10141 cgagttgcca tcggttgcaa acgattcacg ctggcggacc atgagcggac ctcccaaggc
    10201 gagaaaccgg ccgaataacc tacagtttta taccacccgt atttcgatac cccgcggcga
    10261 agctttgatg aaacccgccg gcggcgttta gaatcggctg taaccgcttg tcccgccggc
    10321 agttgcgccg cggaggaagc ttctggtgaa gagcgctcgc tgggccacgc tgctggccgt
    10381 tgccatcccg atcgcggtgc tgttgggccc gctgctcgtc accgaccgca gcttcgcgat
    10441 gcgcgacgcg gcccatttct actatccgct ttttgcctgg tgcgcccgac agtggagcgc
    10501 cggcagcgtg cccctttgga atccgtacga gaactgcggc ctgccggtgc tggccgatgc
    10561 atcgtcgagc atcttctatc ccggcaagct gctgttcttg ctcccagtca atttcgcgct
    10621 gcgctacaag ctctatgtcg tgctgcacgt cgtgctcgcc gcggcgggct cttacgcgct
    10681 cgcgcggcat tggaaggcca gcccgcaggc ggccgcgctg gcagcgattg cgtactcctg
    10741 cggggggaac gtcgtctttc aatactgcaa cgtcgtgttc ctggtcggtg cagcctggct
    10801 gccgctggcg gcgctcgcgg ctgaccggat gctcgccggc cgcagctggc tctcggcggt
    10861 cctgctgggc ctggcgctgg cgctgatgat cctgggcggc gatccgcagg cggcgtatca
    10921 ttcgctgctc atcacagcgt tgtatgcggt cgtgatcgcg ctggcgcgcg gcgacgcgac
    10981 gcactcggcc acggatcggt tccgcaggct gggactggga atcggattga cgggcaccgc
    11041 cgcgatcgtc ggctttctgc tggccgcggt gcaagtcttg ccctcctccg aagcgacaca
    11101 gtacagtgag cgcgcggccg tcgaccggcc aaggaacatc tatgaagcgc tgaccaccgc
    11161 cgcgcccgcg ggcgagtcgc gcccgcaagc gatcgccaag ggcttcttcg gcaagccgga
    11221 ccgcggcacg catcaggacc tggcgtacga cttcagcgtc ggcccgtggc ggctggtcga
    11281 atatctctgg ccaaacgttg ccggccgaat gctgccgacg aatcgccgct ggttttcgct
    11341 cctgccgatg gaaggccgca cctggacgcc cactctgtat ctgggcctcg tgccgctgct
    11401 gctcgggctg gctggctttc gcctgtgggg ccgcgcggag ccgcggcagc gctggctttc
    11461 gtggctggtg ctgatcttca cgctcggcag ctttggctac tacggcctgg gttggctcgc
    11521 gaccgaggcc tacgcaacgc tgtttcgcgg cgacgcggcg aagatcgact tagctcccgg
    11581 agtgggcggt gtctattggt tcttcgtcac gttcctgccg acgtacgttt atttccgcta
    11641 tccggcgaag ctgctgccgc tcgtgtcgct ggggctgagc cagctggcgg cccttggttg
    11701 ggataggatg cacgcgcagc ctcagcggcg actgaccgct gttctgctca tcctcggcat
    11761 tctctcgggc atcctggcgt tcatcatctg gtccatcgac ccgactttca tttcggccct
    11821 ggtgcgcgga cggggcgatt cctcgctcgg ccctttcgac gccgctggag cccgccgcga
    11881 tgtattgttc gctttccttc aggcggcagt cgttgcgctc tgggcgcgct ggctgctgct
    11941 gaagtcgtgg aattcaccaa cgcagcaggc ccgatggcag tggtcgctgc tcgtgctgac
    12001 tgccattgaa gttgctgtgg ccaacgcctg gctggtagtc gctgcgccgg cctcggtgtg
    12061 gcgcgacccg tcgcctgtgg ccgcagcgat tcaagccgca tcggacgaga gcgacttgcc
    12121 gccgcgagtc tttcgcgcga atctgggcgg ctggcgaccg ccgagctttc gccagaagtc
    12181 gtccgccgag cgtcccgcgg aatcggccgc ctgggaaaag gacacgctgt ttcccaagta
    12241 ccacctgccc agccggctgt cgctcgtcga gtcgtacggc tcgatcaagc tgctcgacta
    12301 tgaatcgctc ctgttcgtgg ctcgcgccag cgggccgaaa cagcccgacg gctcgttgct
    12361 cccgcacccc gccgcgctgc ggctggtggg aaccgagttc ctgctgctgc ccgccagcca
    12421 cgagccaaaa ttcgccgagc cgatgcagcc ggcagcgggc acacgctggc ccgaggacgc
    12481 cgtgctgtgg cgaatggcgc ggacgctgcc gcgggcctgg gtcgtgcacg acatcgcggt
    12541 cctccctccc ctgccccgcc cgccgcgcct tgccgcaatc gatcagcgga gcagcgaggt
    12601 gctgttcccc gaccgcaagt cccgcgactt cctgcgcacg gcggtggttg aaaccgatca
    12661 gccgctggcg gagtggaacg cgccgccggc cgagagcccc accaccgcgg agccctgccg
    12721 catcacgcac tacgatccgc agcgggtcgt cgtcgaggtc gagcttgccc ggccgggcct
    12781 gctcgtcctg ggcgaagcct ggtaccccgg ctggcagg
//
LOCUS       DASRJB010000880         8184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3364081, whole genome shotgun sequence.
ACCESSION   DASRJB010000880 DASRJB010000000
VERSION     DASRJB010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3364081"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..766)
                     /locus_tag="VFB80_23160"
     CDS             complement(206..766)
                     /locus_tag="VFB80_23160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZN36756.1"
                     /translation="MKIYTKTGDAGETGLFGAKRVRKDDVRIEAYGTVDELNAALGLV
                     RAETLPEPLGHTLQRVQSELFSVGAELATPDPFKHGTALVGEPHVVQLEHEIDHLEAG
                     LAPLKRFILPAGCRAAALLHVARGVCRRAERRVVTLAAAEPNLQDGRVVRYLNRLGDY
                     LFVAARYANAEARCGETEWIKPKPEK"
     gene            835..1131
                     /locus_tag="VFB80_23165"
     CDS             835..1131
                     /locus_tag="VFB80_23165"
                     /inference="COORDINATES: protein motif:HMM:NF023740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3634 family protein"
                     /protein_id="HZN36757.1"
                     /translation="MFEDLENWIVPLAILGLLAAAFWYAMRPRRVFTLRIANGGVAST
                     VGTVTRSFVRDVEEVCRRAGVPAGEVRGEARGRAIVLAFSGPIPGECRQQLRNL"
     gene            1248..2258
                     /locus_tag="VFB80_23170"
     CDS             1248..2258
                     /locus_tag="VFB80_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HZN36758.1"
                     /translation="MLSAAAVAAQDSIESKFLSNVRQVTRGMVKAGEGYFSPDGRTIV
                     YQAQPLDYPFYQIFTQPLDGENVRPKLISTGRGRTTCSYFSPDGKKILFASSHLDPKI
                     GETETAERKQQEADKQAGVRRRYTWDFDPHTEIFEADLDGKILRQLTHAKGYDAEGAY
                     SKDGQLIAFASDRDGDPDIYLMDADGNNVRQLTNTPGYDGGPFISPDQKWIVFRSDRK
                     KAEWLQLYVIGIDGKNETALTDTTGVNWGPYWHPSAPYIIWAGADHSDPNARPNYDLW
                     LAKYEAAGGKFKLGPPIRVTDFAGTDILPVFSPDGKKLMWTSTRTDDHMSQLFIADFK
                     LP"
     gene            2281..2703
                     /locus_tag="VFB80_23175"
     CDS             2281..2703
                     /locus_tag="VFB80_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN36759.1"
                     /translation="MATQKSCGFLILRGEPVREFLLMRHKDRWDLPKGHVDPGESELQ
                     CALRELREETGLAAADIEPIEGFRFTLEYPVRNKRTGQLDDKTLVVFLARLAREVEIN
                     PSEHPGYQWFPWQPPHRIQQQTIDPLLEAVRQFMVPGH"
     gene            complement(2713..3576)
                     /locus_tag="VFB80_23180"
     CDS             complement(2713..3576)
                     /locus_tag="VFB80_23180"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HZN36760.1"
                     /translation="MHDAQGMALVFALLVVGALVAAVLAMARYQQTHQLLERVAVRFR
                     GQIEPGDFFTSPEVRLKFEGHAAVLRFKRVGKHSHQTIFSIAWPDQGLRCEVYPQDIY
                     SGLRKLLGTEDIEIGSPQFDATYFIAGNSPAEIRGLLTSEVQSVVFRLSSLSQANYFA
                     TREIQVKWGGGVMTVIKPSRLSTFESLEQFVSLCGMLFSAAIRTRNTGIEFLAENREP
                     DTAEAQCQVCGAGLTGDLVYCASCETPHHRDCWTYFGSCSTYACGEKRFTERPKPARP
                     AKQEKKHRQRK"
     gene            complement(3608..4402)
                     /locus_tag="VFB80_23185"
     CDS             complement(3608..4402)
                     /locus_tag="VFB80_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36761.1"
                     /translation="MSVSCKLPPSWRDFEVFRAVIVGGKSTREAAELFRLSQTRICQI
                     VERTKAWQAEVLPAECPLPEEGRLRLSQNIAVERIDSLYCEVMGAWRKSQGEVKQTRT
                     SRYGDEVTTVKTSCGDSKYLLAAMRLVTASAEIGRHGGLALPDDEVGWAVPTAAQEGV
                     VGTAHPTPKEDCSPNGRSQPIPPPSPDAADDATATDDNTSRELSPPQRLARRVLMAPV
                     QHDHAAALASSKGGTRLDISPSQPGVISRPLTRQQRRKLQRALRVR"
     gene            complement(4669..5610)
                     /locus_tag="VFB80_23190"
     CDS             complement(4669..5610)
                     /locus_tag="VFB80_23190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZN36762.1"
                     /translation="MIGGVGERSLAGGLRYSVQGGSYLAYRNLFSWDVLPTVAQFQTA
                     VEQAFNAWSSVDPATGLTTSLAFIADFGTGVIGANTGNGGLDVGGAEIDLFGSNDAFF
                     WNVGNGGTQAETSFGAIADTVTLTSGTANYAGSTAISGADIIMNSNSQAVYSLDVFRR
                     ILTHEIGHALGLGDVEGDINPGAFIDDNYNSANSATALATLTNSWALLINPLNPGASA
                     GLQRYTVAFGDPGTTTTNVDILMESRGVGIGPTNPVTSLVPLTNDDYGTRQFLYPSLL
                     VQAVPEPASILSLSAGLLALCGCCRRRRPKATALSGA"
     gene            6055..>8184
                     /locus_tag="VFB80_23195"
     CDS             6055..>8184
                     /locus_tag="VFB80_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36763.1"
                     /translation="MKTFLAGVALVVMANLAFAGEARLVRYPHYHKGQVAFTYMGDIW
                     TASESGQNIQRLTAHRGRDMIPRFSPDGKWIAFSSDREGNLDIWVVPAEGGSPKQLTF
                     HSSDDSVLGWSPDSKQVLFYSSRGDDFLGTLYVVPLAGGGESKAGTDFGLYGCFSPDG
                     TKLAVNRKGQTYWRKGYRGSYQTDVTVADLKEKTFQDVTNHNGMDTWPMWSSDGSIYF
                     VSDRDENAQPNLWKVPEGGGDAVRVTNFKDGDIRFPAMSADGKTIVFERDFRLWKLDV
                     AGDKAKGEPAAITLAINAEPQDTLTEYRTVNSDVDDYDVANSGRSIVASVRGELFLVP
                     TGEDGDLTQVTKGAARDRNVEFSPDGKSIVYVSDASREREELYLVPADGSGEPKRLTD
                     LNALKTSYSWSPDGKLLVVTTSDGKLYRASADGSETKEILASKYGTIGRPAWSPDGSL
                     LAFSMSDVTRTDDIYIMPAGGGEPKKATFDSASDRSPAFSADGKKLYFIRSDSFGGEG
                     GGFGGVQSGLMVVLLEKQDRDPTEPAAEGGEGQGRRGGGGFGGGGAGGDIANPKPPEI
                     DWLGLKRRTRSVLGGGGGGGRRGGGGGTGGGGTGGVSVSSFTPARDGRTIYFAGSSGG
                     SLGLYSCTDDGRNLRQIASTAPTPTEGDEEPQRGRGGFGRGGMSNLRVTRDGRIYYQQ
                     GNGVYSAAAGGGGGGGGTATTGRGFGGR"
BASE COUNT         1466 a         2645 c         2685 g         1388 t
ORIGIN      
        1 agttgaccaa taccgcagcg cacggcgacc ccatttgacg ccggaaaccg cgccgctcca
       61 aaggatgctc tcgcctgtcg gcctgcaagg cccgccagtc gcctgccgag agaatttcga
      121 aataagctac ttacccggcc gtgattcgta aagggatagt gagagaaaat ttctcgacct
      181 cttttcgctc taagttgcct tccgttcatt tttccggctt tggcttgatc cactccgtct
      241 cgccgcagcg ggcttcggcg ttggcatagc gggcggcgac aaacaaatag tcccccagcc
      301 ggttgaggta ccgcacgacg cgcccgtcct gcagattcgg ttcggctgcg gccagcgtga
      361 cgacccgccg ctccgcccgc cggcagacgc cgcgggccac gtgcaacagc gccgctgccc
      421 ggcagccggc gggcaggatg aaccgcttca gcggcgcgag gccggcctcc aggtggtcga
      481 tttcgtgttc aagctggacc acgtgcggct cgccgacgag cgccgtgccg tgcttgaacg
      541 gatcgggcgt cgccagctcg gccccgacag aaaacagctc gctctgcacc cgctgcaggg
      601 tgtggcccag cggttccggc agcgtttcgg cgcggaccag ccccagcgcc gcgttgagct
      661 cgtccacggt gccataggcc tcgatccgca cgtcgtcctt gcggaccctt ttggccccga
      721 agagccccgt ttcgcccgca tcaccggttt tggtgtagat cttcatggca gcgattccgc
      781 acgacagaca acgcacagca ttatgctaca cttgcgctcc tgcagggtct ggctatgttc
      841 gaggacctgg aaaactggat tgtgccgctg gcgatcctgg gcctgctggc cgcggcgttc
      901 tggtacgcga tgcggccgcg gcgggtcttc acgctgcgga tcgccaacgg cggcgtggcc
      961 tccacggtcg gcacggtcac gcggagcttc gtccgcgacg tggaggaagt ctgccgccgc
     1021 gccggtgtgc cggccggcga agttcgcggc gaagcccgcg gccgggccat cgtgctggcc
     1081 ttctccggcc cgatccccgg cgaatgccgc cagcagctgc ggaacctgta gcaaatgcac
     1141 ggatcgctgc ggtaacgggg gaagaaccaa agaaccgaag aaccaaaatt ccgaacacca
     1201 aacccaatac accaaacacg atgcgaaccg cctgcttgtc cttgctcatg ttgtctgctg
     1261 ccgccgtcgc ggcccaggac tcgatcgaaa gcaagttcct ctcgaacgtc cggcaagtga
     1321 cgcggggaat ggtcaaggcc ggcgaaggct acttctcgcc ggacggccgc acgatcgtct
     1381 atcaggcgca gccgctggac tatcccttct atcaaatctt cacgcagccg ctggacggcg
     1441 aaaacgtgcg gcccaagctc atcagcacgg gccgcgggcg gacgacgtgc agctacttct
     1501 cgccggacgg caagaagatc ctgttcgcct cgagccatct cgatcccaaa atcggcgaga
     1561 ccgagacggc cgagcgcaag cagcaggagg cggacaaaca ggccggcgtg cggcggcggt
     1621 acacgtggga cttcgatccg cacaccgaga ttttcgaggc cgacctggac ggcaaaatcc
     1681 tgcggcagct cacccatgcc aagggctatg acgcggaagg ggcgtattcc aaggacggcc
     1741 agctgatcgc ctttgccagc gaccgcgatg gcgacccgga tatctatctc atggatgcgg
     1801 acggcaacaa cgtccgccag ctcacgaaca ctccgggcta cgacggcggg ccgttcatct
     1861 cgcccgacca gaagtggatc gtcttccgca gcgaccgcaa gaaggccgag tggctgcagc
     1921 tgtacgtgat cggcatcgac gggaagaacg aaactgccct gaccgatacg accggcgtga
     1981 actggggccc gtactggcat ccgtctgctc cctacatcat ctgggctggg gccgatcatt
     2041 ccgatcccaa tgcccgaccc aactacgatc tctggctggc gaagtacgaa gccgccggtg
     2101 gcaagttcaa gctcggtccg ccgatccgcg tcaccgactt cgccggcacc gacatcctcc
     2161 cagtgttctc gcccgacggc aaaaagctga tgtggaccag cacgcggacc gacgaccaca
     2221 tgagccagtt gttcattgcg gatttcaagc tgccatagga tcgactcgtt gggcgtattc
     2281 atggccacgc aaaagtcctg cggctttctg atcctccgcg gcgagcctgt ccgcgagttc
     2341 ctgctgatgc ggcacaagga ccgctgggat ttgccgaagg ggcacgtcga tccgggcgag
     2401 agcgagctgc agtgtgccct ccgcgaactg cgcgaagaga cgggcctggc cgccgccgac
     2461 atcgaaccaa tcgagggctt tcgcttcacg ctcgagtatc ccgttcgcaa caagcgcacc
     2521 ggccagctcg acgacaaaac gctcgtggtg ttcctcgccc gcctggcccg cgaagtcgag
     2581 atcaatccca gcgagcatcc gggctatcag tggtttccct ggcagcctcc gcaccgcatt
     2641 cagcagcaga cgatcgaccc gctgctggag gctgtgcggc aattcatggt gcctggtcat
     2701 tagtttgcct ggtcatttgc gttggcgatg ctttttctcc tgcttcgcgg gcctggccgg
     2761 cttgggtcgt tcggtgaacc tcttctcgcc gcaggcgtag gtcgagcagc tgccaaaata
     2821 ggtccagcag tcgcggtggt gcggcgtctc gcagctcgcg caatagacga ggtcgccggt
     2881 cagcccggcg ccgcagacct ggcactgggc ctcggcggtg tcgggctcgc ggttctcggc
     2941 tagaaactcg atgcctgtgt ttcgcgtccg gatcgcggcc gaaaacagca tcccgcacag
     3001 cgagacgaac tgctccaacg actcgaaggt cgaaagccgg ctgggcttga tgacagtcat
     3061 caccccgccg ccccatttga cctggatctc gcgggtggca aagtagttgg cctggctcag
     3121 cgacgacagc cggaacacga ccgactgcac ttccgacgtc aacagtccgc ggatctccgc
     3181 cgggctgttg ccggcgatga aataggttgc gtcgaactgc ggtgagccga tctcgatgtc
     3241 ctcggtgccc agcagcttgc ggaggccgct gtagatgtcc tgcggataga cttcgcaccg
     3301 caggccctgg tcgggccagg cgatcgagaa aatcgtctgg tgcgagtgct tgccgacgcg
     3361 cttgaaccgg agcaccgccg catgcccttc gaatttgagg cgcacctcgg gactggtgaa
     3421 gaaatctccc ggttcgatct gtccgcgaaa gcgaaccgcg acccgctcca gcagctgatg
     3481 cgtctgctgg taccgcgcca tcgcgaggac cgccgcgacc agcgcaccga ccaccagcag
     3541 ggcaaagacc agggccatac cttgggcgtc gtgcatggtg gaatcagctc ttcagggtgc
     3601 cgtcaaccta cctcactcgc agcgcgcgct gaagtttgcg gcgctgctgg cgggtgaggg
     3661 gtcgcgagat cactccgggt tggctcggag aaatgtcgag ccgcgtgcct cccttggagg
     3721 aggcgagtgc cgccgcgtga tcgtgctgaa caggggccat cagcacgcgg cgggcgagcc
     3781 gctgcggtgg gctcaactcg cgggaagtat tgtcgtccgt ggcagttgcg tcgtcggctg
     3841 cgtctggtga gggcggtgga atcggctgag accggccatt gggtgaacag tcctccttcg
     3901 gggtagggtg ggctgtgccc accacgccct cttgcgccgc ggtgggcaca gcccacccta
     3961 cttcgtcgtc cggcagtgcc agcccgccgt ggcggccgat ctcggccgag gctgtgacca
     4021 gtcgcatggc ggcgagcaga tacttggaat cgccgcacga agtcttcacc gtcgtcacct
     4081 cgtcgccgta tcgggaggtg cgggtctgct tgacttcgcc ctgcgacttc cgccacgcgc
     4141 ccatgacctc gcagtagaga ctgtcgatcc gctcgacggc gatgttttgc gatagccgca
     4201 ggcggccctc ttccggcagc gggcattcgg ccggcagcac ttcggcctgc caggctttcg
     4261 tgcgctcgac gatctggcag atgcgagtct gcgagagacg gaacagctcg gccgcttcgc
     4321 gggtcgattt cccgccgacg atcacggccc gaaacacttc gaaatcccgc caactcggcg
     4381 gcagtttgca ggaaacgctc atggcaattc tccgggtgat ggtggtgacg aaagcaccat
     4441 ctggacagaa ttgctaaaag ccgtcaaacg cgcgccgcgc ggattttttc gcccgaccga
     4501 ttttccgcgc cgcaacctca agcagagtaa tgctttgcgc tcaggaaaat aggccagaaa
     4561 gctgctgcag atttggacta gtctggtccg cagcgacccg ccctaagtgt gtacggattt
     4621 actaccgctt tgcccgggtc tccgggcgca aggtgcattt cgctccgctc atgcacccga
     4681 cagcgctgtt gccttcggcc gcctgcgccg gcagcagccg cagagtgcca agaggccggc
     4741 gctcagcgag aggatggaag ccggttcggg aacggcctga accagcaagg aaggatagag
     4801 gaactgacgc gtgccgtagt cgtcgttggt caaggggacg agactggtga cgggattggt
     4861 cggaccaatg ccgacgccgc gcgactccat caggatatcc acgttggttg tggtcgtgcc
     4921 cgggtcgccg aacgcgacgg tgtagcgctg caagccggcg gaggctcctg ggttcagagg
     4981 attgatcagc aaggcccagg agttggtgag cgtggccagg gccgtggcgc tgttagcgct
     5041 gttgtaattg tcgtcgatga atgcgccggg gttgatgtcc ccctcgacgt cgcccaggcc
     5101 aagcgcatgt ccgatctcgt gcgtcaggat gcggcggaaa acgtcgagcg aataaacggc
     5161 ttgggagttg ctgttcatga tgatgtcggc gccgctgatg gcggtggagc ccgcgtagtt
     5221 ggccgtgccc gacgtcaagg tcaccgtgtc ggcgatagcg ccgaagctgg tttccgcctg
     5281 cgtcccgccg ttgccgacgt tccagaagaa ggcatcgttg gagccgaaca ggtcgatctc
     5341 ggcgccgccg acatccaagc cgccgttgcc ggtattcgcg ccgatgacgc cggtgccaaa
     5401 gtctgcgatg aaagccagcg acgtggtcag gccggtcgcc ggatcaacgc tggaccaggc
     5461 attgaacgcc tgctccacgg cggtctggaa ctgagccact gtcggcagga cgtcccagct
     5521 gaacaaattg cggtaggcta ggtaagaacc gccctgcacg gagtagcgga ggccgccggc
     5581 gagcgaccgc tcccccacgc cgccaatcat ccgcggggcc gcgtcccacc gcgagccgcc
     5641 gccgaggttg gcgtccgaga aattgaaaat cacacccgcg ctggccagct gcgtccctcc
     5701 caacaggcag gcggcacatg cagccgcagc ccagaggccg atccatcgtc ggtggaatac
     5761 acgttgcggc atcggcgtcg tctcctcgcg gcagctcaaa aatgcgtttt agttagcgct
     5821 ctgcgctggg ccataggcac tcactatctg tttgccggca gggcgccaaa cctgacaggc
     5881 aaaaattcga aaaatcctgc cgcttgctca gaaaacccgg ccggtgggca cagcccacgc
     5941 tctagtcccc ggttgctttc ggccggctac gccgcggact atgatcgtag acgcgcacgc
     6001 aggcggcatt cgcgcctgcc cttttctgtg gcccctgctc gaacaggccg aaccatgaag
     6061 acgtttctcg cgggcgtggc gctagtggtc atggcgaatc tggctttcgc cggcgaggcc
     6121 cggctggtgc gttacccgca ctatcacaag ggccaggtcg ccttcactta catgggcgat
     6181 atctggaccg ccagcgagtc ggggcagaac atccagcggc tgaccgccca tcgcggccgc
     6241 gacatgattc cccgcttctc gcccgacggc aaatggatcg ccttttccag cgaccgcgaa
     6301 ggcaatctcg acatctgggt cgtcccggcc gaaggcggct cgcccaagca gctcactttt
     6361 cactcgtccg acgacagcgt cctcggctgg tcgcccgaca gcaagcaggt cctgttctat
     6421 agcagccgcg gggatgactt cctcgggacg ctttacgtcg tgcccctcgc gggcggcggg
     6481 gaatcgaagg ccggcaccga cttcggcctg tacggctgct tctcgcccga cggcacgaag
     6541 ctggccgtca accgcaaggg gcagacgtac tggcggaagg gctatcgcgg ctcgtaccag
     6601 accgacgtga cggtcgccga tctcaaggag aaaacgttcc aggacgtcac gaaccacaat
     6661 ggcatggaca cgtggccgat gtggagcagt gacggctcga tctatttcgt cagcgaccgc
     6721 gacgagaacg cgcagccgaa cctgtggaaa gtgcccgagg gcggcggcga cgcggtccgc
     6781 gtgacgaact tcaaggacgg cgacatccgc ttcccggcga tgagcgccga cggcaagacg
     6841 atcgtcttcg agcgggattt ccggctgtgg aagctcgacg tggctggaga caaagccaag
     6901 ggggagccgg cggcgatcac gctggcgatc aacgccgagc cgcaggacac gctgaccgaa
     6961 taccgcacgg tgaacagcga cgtcgatgac tacgacgtcg ccaacagcgg ccgcagcatc
     7021 gtcgcgtcag tccgcggcga actgttcctc gtgccgacgg gtgaagacgg cgacctgacg
     7081 caggtcacca agggggcggc ccgcgaccgc aacgtcgagt tctcgcccga cggcaagtcg
     7141 atcgtctacg tctccgacgc cagccgcgag cgggaagagc tctatctcgt gcccgccgac
     7201 ggctcgggcg agccgaagcg gctgaccgac ctcaatgccc tcaagacgag ctactcgtgg
     7261 tcgcccgacg gcaagctgct ggtcgtgacc acctccgacg gcaagctcta ccgcgccagc
     7321 gccgacggca gcgaaaccaa ggagatcctg gcgtcgaaat acggcacgat cggccggccg
     7381 gcgtggtcgc ccgacggctc gctgttggcg ttcagcatgt ccgacgtgac gcggaccgac
     7441 gacatctaca tcatgccggc gggcggcgga gagccgaaga aggccacgtt cgattcggcc
     7501 agcgatcgca gcccggcgtt ttcggccgac ggcaagaagc tctacttcat ccgctccgat
     7561 tcgttcggcg gcgaaggggg aggttttggc ggcgtgcagt cgggcctgat ggtcgtgctg
     7621 ctggagaagc aggatcgcga tcccaccgag ccggctgctg aaggcggcga aggccagggc
     7681 cggcgcggcg gcggtggctt tggcggtggc ggcgccggcg gcgacattgc gaatcccaaa
     7741 ccgcccgaga tcgactggct gggcctgaag cgccgcacgc ggagcgtgct gggcgggggc
     7801 gggggcggcg gacggcgggg cggcggtggc ggaaccggcg gaggtggtac tggcggcgtt
     7861 tccgtctcga gctttacgcc ggcccgcgac gggcggacga tttatttcgc cggcagctcg
     7921 ggcggcagcc tgggtcttta ctcctgtacc gacgacggcc gcaacttgcg gcagatcgcc
     7981 agcacggccc cgacgccgac ggagggcgat gaggaaccgc agcgcggccg cggcggattc
     8041 ggccgcggcg gcatgagcaa cctccgcgtc acccgcgacg ggcggatcta ctatcagcag
     8101 ggtaacggcg tctattcggc ggcggccggc ggcggcggtg gcggcggagg aaccgcgacc
     8161 actggtcgcg gctttggggg gcgc
//
LOCUS       DASRJB010000881         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3925383, whole genome shotgun sequence.
ACCESSION   DASRJB010000881 DASRJB010000000
VERSION     DASRJB010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925383"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFB80_23200"
     CDS             <1..935
                     /locus_tag="VFB80_23200"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36764.1"
                     /translation="RTIHEARQLMLAARKAKVVTQMGNQGHSGYGYRALVALVQGGAI
                     GKIKEAHAWSNRPIWPQGIDRPPGSSPVPETLNWDLWLGVAPERPYVDKAYAPFNWRG
                     WYDFGAGALGDMGCHILDPVVWSMELPAATRVYYGGPAAKPEAFPEWERIHYEFPATR
                     YTDGPIHVIWYDGKQSGEQVLPFAELAPGAKIPTNGILLVGEKGTLLCEHGGSPKLLP
                     EDKFKDVEQIKLPAQDHYLAWTNAIRGEGTTTSNFDYAGPLTETVLLGTIASRFPGEQ
                     LEWDSANLKFTNVPKANDYVQQPYRKGWEVPGLS"
     gene            993..3143
                     /locus_tag="VFB80_23205"
     CDS             993..3143
                     /locus_tag="VFB80_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36765.1"
                     /translation="MLIRLTAALLVIGLAPATLAETPVDLSGYRADCAVRVEGWNGNL
                     RVSWPMEAGERGEVTLDLSRTKPLIQRLATQKGDAEPAAILGGLDPAWYLTVGERRPA
                     AEKPPDQQWEVFFDNPHQRPHEVHASQLAITKARVTGSGQRATVAIDGLTVGPFTGSI
                     EFRFFAGSRLLRIDAVISTQKDRLAVFYDEGLVAADAKWREVTWVDTEGRTQRKSISG
                     LTPTARLEKAEPLKVRHRPIVAGGESGAVACFPPPHQFQFPRDYSTNLGFVWHGAGYQ
                     GQAGKTGFGIRQNKDGGGNFVPWFNAPPNTQQRMGMFLLFSSGSADEALRETLQLTNN
                     DRFPELPGYKTFTSHYHMAIAVKAMEERAKGFNRTEPPDYVRVFQDMNVNMVHLGEFH
                     GDGHPKDPGSLRLPEVEAMFGECSRWSDDRLLLIPGEEINDFLGLKITGKHPGHWMSL
                     FPRPVYWTMQRSAEQPFVEDHPKYGKVYHVGDRGDMIRLLKEERGLAWSAHPRIKASS
                     WTPDIFRQEDFYLADYWLGAAWKAMPGDLSRDKLGERVLNLLDDMANWGQKKYVLGEV
                     DVFTIDHTHELFGHMNINYVQLDRVPRYADGWQSVLNVLRDGRFFVTTGEILLPQFTV
                     GGKPSGDVLKLAAGEQPELTAEVRWTFPLAFAEVISGDGQQVYRERIDLADTAAFGTR
                     TLQLRPKLTGRRWVRLEIWDIAANGAFTQPVWIE"
BASE COUNT          598 a         1104 c         1086 g          539 t
ORIGIN      
        1 cccgcacgat ccacgaagcc cgccaactca tgcttgccgc ccgcaaggcg aaggtcgtga
       61 cccagatggg caaccagggg cacagcggct acggctatcg cgcgctggtg gccttggtgc
      121 aggggggcgc gatcggcaag atcaaagagg cccacgcctg gtcgaatcgc ccgatctggc
      181 cgcaggggat cgaccgcccg ccgggcagct cgccggtgcc cgagacgctg aactgggatc
      241 tctggctcgg cgtcgctcct gagcggccgt acgttgacaa agcgtatgcc ccgttcaact
      301 ggcgcggctg gtacgacttc ggggccgggg cgctgggaga catgggctgc cacatcctcg
      361 acccggtcgt gtggagcatg gagctgcccg cggcgacgcg agtctattac ggcggcccgg
      421 cggccaagcc cgaggcgttt cccgaatggg agcggattca ctacgaattc cccgccacga
      481 gatacacgga cggtccgatt cacgtcatct ggtacgacgg caagcagagc ggcgagcagg
      541 tgctgccgtt tgccgagctc gcgcccggag caaaaattcc gaccaacggc atcctgctgg
      601 tcggcgagaa gggcacgctg ctctgcgagc acggcggcag ccccaagctg ctgcctgagg
      661 acaaattcaa ggacgtcgag caaatcaagc tgccggcgca agaccactac ctggcttgga
      721 ccaacgcgat ccgcggcgag ggcacaacga cctcgaactt cgactacgcg ggcccgctca
      781 ccgagaccgt cctgctcggc accatcgctt cgcgcttccc cggcgagcag cttgaatggg
      841 actcggccaa cctcaagttc accaacgtcc ccaaggccaa cgactacgtg cagcagccgt
      901 accgcaaggg ctgggaggtg ccggggttgt cttgagctgg tcagtggtca gtgggttcga
      961 accaggaata ctccctccgg caaagaactg ccatgctgat tcgcctgacc gcggcgctgt
     1021 tggtgattgg gctggcgcct gcaacgctgg ccgaaacgcc ggtcgatctt tccggctatc
     1081 gcgccgactg tgccgtgcgg gtggaaggct ggaacggcaa tctgcgggtg tcgtggccga
     1141 tggaagcggg cgagcgcggc gaggtgacgc tcgacttgtc gcgtacgaag ccgctgatcc
     1201 agcgactggc gacgcaaaag ggcgacgccg agccggcggc gatcctgggc ggcctggatc
     1261 cggcctggta tctcacggtg ggcgagcggc gcccggccgc ggagaagccg ccggaccagc
     1321 agtgggaggt ctttttcgac aacccgcacc agcggcctca cgaagttcac gcctcgcagc
     1381 tcgcgatcac gaaagcccgc gtcaccggca gcggccagcg ggccaccgtg gccatcgatg
     1441 gcctcaccgt tggccccttt accgggtcga tcgagttccg cttcttcgcc ggttcacggc
     1501 tgctcaggat cgatgcggtt atctcgacgc agaaagaccg gctcgccgtg ttctacgacg
     1561 aggggctcgt ggccgccgac gcgaagtggc gcgaggtgac ttgggtcgat acggagggcc
     1621 gaacgcagcg gaaaagtatc agtgggctca cgcccaccgc tcgcctggag aaggcggaac
     1681 cgcttaaggt ccgccatcgc ccgatcgtcg ccggcggcga aagcggcgcg gtggcctgct
     1741 tcccgccgcc gcatcagttt cagtttccgc gcgactactc gaccaacctc ggtttcgtct
     1801 ggcacggcgc cggttaccaa ggccaggcgg gcaagacggg ctttggcatc cgtcagaaca
     1861 aagacggcgg cggcaatttt gttccctggt tcaacgctcc ccccaacacg cagcagcgga
     1921 tgggcatgtt cctgctattc tcgtccggct cggccgacga agcgcttaga gagacgctcc
     1981 agctcaccaa caacgatcgc ttccccgagc tgccgggcta caagaccttc accagccact
     2041 accacatggc gatcgccgtc aaggcgatgg aggagcgggc caagggattc aaccgcaccg
     2101 agccgcccga ctatgtccgc gtcttccagg acatgaacgt caacatggtc cacctgggcg
     2161 aattccacgg cgacggccat cccaaagatc ccggctccct gcggctgccg gaggtcgagg
     2221 cgatgttcgg cgagtgcagc cgctggtcgg acgaccggct gctgctcatc cccggcgagg
     2281 aaatcaacga cttcctcggc ctgaagatca cgggcaagca cccgggccac tggatgagtc
     2341 tctttccgcg gcccgtctat tggacgatgc agcgctccgc cgagcagccg ttcgtggagg
     2401 accatccgaa gtacggcaag gtctatcacg tcggcgaccg cggcgacatg atccgcctcc
     2461 tcaaggagga gcgggggctg gcctggagcg ctcatccgcg gatcaaggcc agcagctgga
     2521 cgcccgacat cttccgccag gaggattttt atctggccga ctattggctg ggagcggcgt
     2581 ggaaggcgat gcccggcgac ctgtcgcgcg acaagctcgg cgagcgggtg ctcaacctgc
     2641 tcgacgacat ggccaactgg ggacagaaga aatacgtcct gggcgaggtc gatgtcttca
     2701 ccatcgacca cacgcacgag ctcttcggcc acatgaacat caactacgtg cagctcgatc
     2761 gcgtcccgcg ctacgccgac ggctggcaga gcgtgctcaa tgttctccgc gacggccgct
     2821 tcttcgtcac cacgggcgaa atcctgctgc cccagttcac cgtcggcggc aagcccagcg
     2881 gcgacgtgct caagctggct gccggcgagc agccggagct cactgccgag gtccgctgga
     2941 cctttccgct ggcctttgcc gaggtgatct ccggcgacgg ccagcaggtc tatcgcgagc
     3001 ggatcgacct ggccgacacc gccgccttcg gcacgcgcac tctccagttg cgtcccaagc
     3061 tcaccggccg ccgctgggtg cgcctcgaaa tctgggatat cgccgccaac ggcgcattca
     3121 cgcagccggt gtggattgag taacctgacc actacctttc aacgagggcc gccatgagag
     3181 tgttgtgtgc cgtcacagcc gtgttctact tcgtatcgct gtccgcagcc gccgactggc
     3241 cgcagtggcg cgggccggag cgcaatgggg tctcgaagga gacgggcctc ttgcaggaat
     3301 ggcctgcggg cgggccgaag ctgctct
//
LOCUS       DASRJB010000882         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1951627, whole genome shotgun sequence.
ACCESSION   DASRJB010000882 DASRJB010000000
VERSION     DASRJB010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951627"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFB80_23210"
     CDS             complement(<1..555)
                     /locus_tag="VFB80_23210"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HZN36766.1"
                     /translation="MPRFAVILPAAGKSSRFRDQHYKKPFAPLDGRPVWMHVADRFAN
                     RAEVAQTLLVIAPEDREYFAEKFAGNAAMLGIEVVEGGAERYDSVQKALARVSADIDF
                     VAVHDAARPCVAAEWIDAVFAAAVKTGAAILAVPVQGTVKRANPDQTIAETVARDKLW
                     EAQTPQVFRRQLLIDAYARRGQQPA"
     gene            complement(640..1602)
                     /locus_tag="VFB80_23215"
     CDS             complement(640..1602)
                     /locus_tag="VFB80_23215"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZN36767.1"
                     /translation="MGTLTGDFGLAASLYYQLRAIGFRMADGVIVSSKLEQHSDSEGT
                     SYTPAVEYRYSVGRQEHVGRRINYSWVATSSQGARQAIAQYPAGQRVTVYYDPRRPED
                     SLLEPGLHSGQAFLALFLLPFNIIMATCWLAVWQWFRGTAGRLPEGARATATPEGFVL
                     RLYRYSPLAAAALAALFVSFAGVFIAAFGSGAMPQSVLVGCAWVVVSAGSTWAYRRQY
                     QRAVLLTVDELRGHYRLQPAAAGRAERAGPLQELSGVTVREHQKLDSDGDLTKKYSVA
                     LVRRSKSGAGRHCCLVTGWSEQPARQLADWLTERLKLPAVPSRA"
     gene            complement(1727..>2422)
                     /locus_tag="VFB80_23220"
     CDS             complement(1727..>2422)
                     /locus_tag="VFB80_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN36768.1"
                     /translation="FMKTHGGRSFLHGHLLGDNVHEYLAPCIYEGEGEMLGMALFKSL
                     VKQHGTEFFEPVGKALAAAGMKTFSPANPAHVWKLRQVLVPYASWTLREMFASKSHSE
                     LGNLSPQLREHALFAIRGLQRMPLEISGAMRKHQLKLADRQCRMSEISARVQKLVVML
                     ATSLFATPQSDEVVKAAADVMCQGLASELTGQRPTDRYFRALNNLGEAIADGKFTSIA
                     GLAPDEILMKYEA"
BASE COUNT          418 a          807 c          814 g          383 t
ORIGIN      
        1 cgcgggctgt tgtccgcggc gggcgtaggc gtcgatcaag agctgccggc gaaagacctg
       61 cggcgtctga gcttcccaga gcttgtcgcg ggcgacggtc tcggcgatgg tctggtcggg
      121 attggcccgc ttgacggtgc cctgcaccgg aaccgccagg atcgccgcgc cggtcttaac
      181 cgccgcggcg aatacggcgt cgatccactc ggcagcgacg caggggcggg cggcgtcgtg
      241 cacggcgacg aagtcgatgt cggcgctcac gcgggccagg gccttttgca ccgagtcgta
      301 ccgctccgcg cccccctcga caacctcgat cccgagcatc gcggcgttgc cggcgaactt
      361 ttcggcaaag tactcgcggt cttcgggagc gatcacgagc agcgtctggg ccacctccgc
      421 ccgattcgca aaacggtccg ccacgtgcat ccacaccggc cgcccgtcga gcggggcgaa
      481 cggctttttg tagtgctggt cccgaaagcg gctgcttttg ccggcagcgg gaaggatcac
      541 agcgaagcgc ggcacggggg gagaaacctc aggcggcgga agacgcattc gggaggggac
      601 tgagaggctc acgttattgt ggccgcccca cgggccgcgt caagcgcgag acgggacggc
      661 cggcagcttg agccgctcgg tcagccagtc cgccagttgc cgggccggct gctcggacca
      721 gccagtgacc aggcagcagt ggcggccggc gccacttttc gagcggcgga ccagcgccac
      781 cgagtatttc ttcgtcaggt cgccgtcgga gtcgagcttc tggtgctcgc gaacggtgac
      841 gccggacagc tcctggagcg gaccggcccg ctctgcacgc ccagctgcgg cgggctgcag
      901 ccgatagtgg ccgcgcagct catctaccgt tagcagcacg gcacgctggt actggcggcg
      961 gtaggcccag gtcgagccgg cgctgacgac gacccaggcg catccgacca gaacgctctg
     1021 cggcatggcg ccgctaccga acgcggcgat aaacacgccg gcgaaggaca cgaagagggc
     1081 tgcgagcgcc gcggccgcca gcggcgagta gcggtacagc cgaagcacaa aaccctccgg
     1141 cgtcgcagtc gcccgcgcgc cttcaggcag ccgcccggca gttccgcgga accattgcca
     1201 gacggccagc cagcacgtgg ccatgatgat gttgaacggc agcaggaaca gggccaggaa
     1261 cgcttgcccc gagtgcagcc ccggttcgag cagcgaatct tccggccggc gcgggtcgta
     1321 ataaacggtg acgcgctggc ccgcgggata ctgggcgata gcctgccgcg cgccctgcga
     1381 gctcgtcgcc acccaggaat agttgatccg ccgcccgacg tgctcctggc ggccaacgga
     1441 atagcgatac tcgacggccg gcgtgtaaga cgtgccctcg ctgtccgagt gctgctccaa
     1501 tttgctggag acgatgacgc catcggccat acgaaagccg atggcccgca gctggtagta
     1561 cagcgaggca gcgagaccaa aatcgccggt cagagtcccc accgaccaaa gagcgatcca
     1621 ggcagacgca aatccgattc cgatagttcc cgtcagaatt ccgcccacac gctgaaatgg
     1681 cgtcttcatc tatcgggaat tcgcccgggc ggctggcagc ttcggttcac gcctcgtact
     1741 tcatcaggat ttcatctggt gcgaggccgg cgatggaagt gaacttgccg tcggcaatgg
     1801 cctcgccgag gttattgagc gcccggaaat agcggtcggt gggccgctgt cccgtcagct
     1861 cactggccag gccctggcac atcacgtcgg ccgcggcctt caccacctca tcgctctgcg
     1921 gcgtggcaaa caggctcgtc gccagcatca cgacgagctt ctgcacgcgg gcggaaatct
     1981 cgctcatgcg gcactgacgg tcggccagct tgagctggtg cttgcgcatc gccccgctga
     2041 tctccagcgg catgcgctgc aggccgcgga tcgcaaacag ggcgtgctcg cgaagctgcg
     2101 gggacaggtt gcccaactcg ctgtggctct tgctcgcgaa catttcgcgc agcgtccaac
     2161 tagcgtacgg cacaagcact tggcgcagtt tccagacgtg agccgggttg gcggggctga
     2221 acgtcttcat gcccgccgcg gccagggctt tgcccaccgg ttcgaagaac tcggtgccgt
     2281 gctgcttgac cagcgacttg aacagcgcca tgcccagcat ctcgccttcg ccttcgtaga
     2341 tgcacggggc gaggtactcg tgcacgttgt cgcccagcag gtggccgtgc aggaacgagc
     2401 ggccgccgtg cgtcttcatg aa
//
LOCUS       DASRJB010000883        11020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1952914, whole genome shotgun sequence.
ACCESSION   DASRJB010000883 DASRJB010000000
VERSION     DASRJB010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952914"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1204
                     /locus_tag="VFB80_23225"
     CDS             485..1204
                     /locus_tag="VFB80_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36769.1"
                     /translation="MSSPYPQSYPQQSYPPPPQQMPPQSSGSGCWIGALIGCLGAIVL
                     SVVLCGVGAWWVKNNVTNLIATVAREAIVSVIKESELEQQEKTEVIAQIDRVVDAVKS
                     GKLKPEELEKLFNEMQDSPIFVLIQIYGVEKVYLEPSGLSAEEKQAGKRVFERAFRGI
                     HEKKIAHSDFEAAMPESMRHQGDDPEMKRPQQVSDEDVKKLIANLKKLADDAQIPDEA
                     FQIDISDELKKDIDKVLAGKE"
     gene            complement(1216..1518)
                     /locus_tag="VFB80_23230"
     CDS             complement(1216..1518)
                     /locus_tag="VFB80_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZN36770.1"
                     /translation="MIALSDHPEGIVLPVRAQPGASKNGLRGVQNGALKVAVTQIAEK
                     GKANQAVIDALAKGLGLKRSQVELLAGETQRDKRFLIRGVTRDELARRIATALDAQ"
     gene            complement(1537..2550)
                     /locus_tag="VFB80_23235"
     CDS             complement(1537..2550)
                     /locus_tag="VFB80_23235"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN36771.1"
                     /translation="MSTVLVTGATGFIGRHLVEALLRRGERVRCLVRGGRRDEALRKL
                     DVELVTAALDQPAELAAAVAGAEAVYHLAGLTQSLTFGELSRVNGEGTAAVAAACAEQ
                     SQPPRLIYVSSLSAAGPAPRGQILIETDPPAPDSDYGRSKLAGENAVRREAAKVPSTI
                     VRPGVVFGPRDPEIVRMLQFIVWARFHLSPGLHPPALSFIYVADLVELLLRAADHGSR
                     LTAGNNGHAGQGIYFATAAEHPTYAELGMIVRPMVRRPWAPVISLPAPVAWCVAGFSE
                     GLSRIRGRADVLNLDKMREALATNWACSGEAASRELGFTPARPLEERLRETIDWYRAE
                     GWL"
     gene            complement(2817..4379)
                     /locus_tag="VFB80_23240"
     CDS             complement(2817..4379)
                     /locus_tag="VFB80_23240"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZN36772.1"
                     /translation="MDSLYRTAPRRVTLAASLVVALAAAIYAVSPAGLLTADSTAVSK
                     PAPTQGLNQANALSEAFRFSADQVIPAVVSIRNETQPQVVKRELRAPRGGRPNTPNLP
                     KEFGDLDPLLKRFFEQIPDGDSFDYDAPQMPGRRSSGSGVIIDAAGVVLTNNHVVAGD
                     GKVTVRLNDGREFVAEKVVTDPGTDLAVVKIKAGGPLPYAKLGDSDSLRVGDWVLAVG
                     QPFGLTDTVTAGIVSAKGRDIGMMKHEEFIQTDAAINPGNSGGPLVNLNGEVVGINTA
                     ISSSTGHFEGVGFAVPVNVARWVTPQLLKEGKVHRAYLGIGIQPVNGELADQLGMATP
                     VGAIVTEVRSDSPAAAAGLKPQDVVIEFGGAKISNVRQLQAMAARSPIGSKLPLAVLR
                     DGKRVELQVTLKEQPANYGELATRPLTAESQSGNFDRLGLQVEPLTADVAKQLGVTAN
                     EGVVIVAVQEDSAAAKAGLEPGMAVVQVGRQTIKSVTEFETAAKNASLDKGVLVLVRT
                     SEGSQFKVLKNG"
     gene            complement(4649..5536)
                     /locus_tag="VFB80_23245"
     CDS             complement(4649..5536)
                     /locus_tag="VFB80_23245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN36773.1"
                     /translation="MDGVTCQRIATNGIHLNVAQAGPADGPLVLLLHGFPEFWYGWRH
                     QIPALVAAGYCVWAADQRGFNLSDKPPRVQDYCLDQLAADAAGLIAAAGRRRALVVGH
                     DWGGVVAWWLAANHPELVERLVIINVPHPLVMRRLILTNPRQTLRSWYVFALQVPWLF
                     EWSLARNDWRDLVRGLRNSSRPGAFTDADFVEYRRAWSQPGAILAMLNWYRAPFRAPP
                     RPPRSKRILPPTLILWGKQDKFVLPVAAERSLQLCDQGQLQFYENATHWLPHEEPAEV
                     NRQILNFAGQAAAPSMTRA"
     gene            complement(5639..5968)
                     /locus_tag="VFB80_23250"
     CDS             complement(5639..5968)
                     /locus_tag="VFB80_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36774.1"
                     /translation="MSETLLFWNPGPQLRLSPAEVAREIVWGEEVAGLIDLDVRAIID
                     RLKAAFPQHDEQSGLLIARAGEGRFEATWTWQHVKVECHDLPPDDRQRLIDTIEAAGC
                     QAYQPKA"
     gene            6062..7405
                     /gene="glmM"
                     /locus_tag="VFB80_23255"
     CDS             6062..7405
                     /gene="glmM"
                     /locus_tag="VFB80_23255"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZN36775.1"
                     /translation="MTEPIISVSGLRGIVGESLTPPVAARYVAALARALPPGPLVVSR
                     DGRTSGPMLAEIVCGTLAACGRNVLYANVAATPTLGVLVRQHEAAGGVQISASHNPAP
                     YNGLKLFNAVGRVLPAAAGQPVLAAYRESSAPWAAFDKLGSVSTIADTTGEHLRLILA
                     AVDAAAIRRRSFRVLLDSNAGAGSLLGLQLLEALGCQVSILGQQPTGQFLHPPEPTAE
                     NLAGVGEQVTAAKADVGFCQDPDADRLAIIDENGRYLGEEYTLAICLDHALRQRKGPV
                     VTNCATSRMSEDLAQIYGVPFHRAAVGEANVVDQMLAQRAVFGGEGSGGPIDPRVGLV
                     RDSFVGMALVLAAMATRNRPVSELAAELPRYEIVKRTVNLAVEKLLAALAAVEQRFAD
                     AKADRLDGLRLDWPSRWLIVRGSNTEPIVRIIAEAPTAVAAEEMAAAACDAVAGV"
     gene            7487..8731
                     /locus_tag="VFB80_23260"
     CDS             7487..8731
                     /locus_tag="VFB80_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZN36776.1"
                     /translation="MERAISFAGYFVMIGVAWCLSGDKRRFPWRVVIVGTLLQFALAV
                     VILDTRPGRIAFAAIGDAFRWVSDLTDVGSKFVFGDRFLEHPFAFKVLPTIIFFSSLA
                     AILYYLGIMQLVVKGMAVVMQRFMGVSGAEALSASANVFLGQTEAPLLVKPYIAGMTL
                     SELNAVMIGGFANISGSLIAAFSAKEFGLDPGHLVTASVISAPASLLIAKVLVPETEQ
                     PATLGKVKMEAPPQGANLIEAAAIGAADGVKLAINVAAMLIAFIALIAMLDAAVQAIG
                     IGLARSTGWTAAAGWTLSGLLGYLFSPFAWIMGIETRDCLRAGELLGKKMFANEFVAY
                     TQLAKWKEESSSVQLSPRTITIMTYALCGFANFSSIGIQIGGLGGMAPERQSDIARLG
                     LRAMLGGTIVNFMMGCIAGVLV"
     gene            complement(8728..9171)
                     /gene="cdd"
                     /locus_tag="VFB80_23265"
     CDS             complement(8728..9171)
                     /gene="cdd"
                     /locus_tag="VFB80_23265"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZN36777.1"
                     /translation="MAASERERLIAAALEVRRRAYARYSKFAVGAAILAADGRMFVGC
                     NVENVSYGLTICAERAATFAAVAAGATEFQLLAIAASGGVTPCGACRQVLAEFAPQLP
                     ILLVDADQPKRIVEANLRELLPAAFASFQPQQAKEPRRGSRKQRG"
     gene            complement(9238..9310)
                     /locus_tag="VFB80_23270"
     tRNA            complement(9238..9310)
                     /locus_tag="VFB80_23270"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9275..9277),aa:Phe,seq:gaa)
     gene            9486..10817
                     /locus_tag="VFB80_23275"
     CDS             9486..10817
                     /locus_tag="VFB80_23275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZN36778.1"
                     /translation="MTDSTKRRSARKPGSDEKLNWRIAWGLPLSYPLFPHQNGRWAKK
                     VRGKLSYFGKVADDPKGAKALELWAEQKDDLLAGRTPRTKTAGTTVAELCDHFMQAKD
                     DLLETGEITRQTRTDYERTTTRIVARFGKTRTVADLTPDDFKDLRSSLAKTLGHVALG
                     NEIQRVRVVFKHGYDTGLIDQPMRYGPLFKRPSKKALRLERAEKGQRMIEAADMRRLI
                     EAADVQMRAMIFLAVNCGFGNSDCGTLPLASLDLAGGWLDYHRPKTGIDRRCPLWPET
                     VAVLAKAIEKRPAPKSPDAEPLVFVTKYGAPWAKETFDNPVTKEFRKLLDDLQLHRAG
                     LGFYTLRHVFRTIADGSRDQPAINSIMGHADASMAAAYRERIDDARLRAVADHVRAWL
                     FPEVVAKEKEAAAVALQAKAEREPRAAKQAKPNRRARPQAVADVSFLRVVG"
BASE COUNT         1929 a         3612 c         3606 g         1873 t
ORIGIN      
        1 aagctgcgat atgggctgtg ggtccacgcc ggagtgcccg cagccgcggc gatcgacgag
       61 cagttcgccg aattcgccaa gatcggcgac gtgccgcaat ctgccgccaa gccctagttc
      121 gtccgcggcg tggaattgtg atctttggta cagtatttgc atcatcgttc cgtttggcgc
      181 ggcgggctgc caatttggcg aagccggcgg tcgggctgtc caaaaacaga gcggcctcaa
      241 aggctaataa gtagtattta ggcggtttca gacgcgtcgc cataaatcgt gtagcagaac
      301 taagttgtgg cggcaaattg gccttcgagg gatttccgaa caacccatcc gggggacagc
      361 aaattgcccc gaagcatgcg tctgcggcgg cgtagggttg tctcgccaga caccacaatt
      421 ctctgcccct ggcagcgaag aatccctgac aagcggcagc cgctaacccg aaatcgagcc
      481 ttctatgtcg agcccctatc cgcagagcta cccgcagcaa tcctatccgc cgcccccgca
      541 gcagatgccg ccgcaatcat cgggtagcgg ctgctggatc ggagcactga tcggctgcct
      601 gggcgcgatc gtgttgagcg tggtcctgtg cggcgtcggc gcgtggtggg tcaagaacaa
      661 cgtcaccaac ctgattgcga ccgtggcccg cgaagcgatc gtctcggtca tcaaggagtc
      721 ggaattggaa cagcaagaga aaacggaagt catcgcccag atcgaccgcg tcgtggacgc
      781 ggttaaatcg gggaagctga agccggagga actcgagaag ctcttcaacg agatgcaaga
      841 ttcgccgatc ttcgtgctga tccagattta cggcgtcgag aaggtctacc tggagccctc
      901 cggactgagc gccgaggaga agcaggcggg caagcgcgtt ttcgagcggg ctttccgcgg
      961 cattcatgag aaaaagatcg cgcactcaga ttttgaggcc gcgatgccgg aatcgatgcg
     1021 gcaccagggg gacgatccgg aaatgaagcg gccgcagcag gtgagcgacg aagacgtgaa
     1081 gaagctcatc gccaacctga agaagctggc cgacgacgcg cagattcccg acgaggcatt
     1141 tcagatcgac atcagcgacg agctcaagaa ggatatcgac aaggtgctgg ccggcaagga
     1201 gtgacttcag ctccttcact gagcatccaa cgccgtggca atccgccgag ccagctcgtc
     1261 gcgcgtcacg ccgcgaatga gaaagcgctt gtcgcgctga gtctcgccgg cgagcagttc
     1321 gacctgcgag cgcttgaggc ccaggccctt agccaaggcg tcgataactg cctggtttgc
     1381 cttgcctttt tcggcgatct gagtcaccgc caccttgagc gccccgtttt gcacgccacg
     1441 caggccgttc ttgctcgcgc cgggttgtgc ccgcaccggc aggacgatgc cttcgggatg
     1501 gtccgacagg gcgatcatgc tcgctccggc aaactcctac agccaacctt ccgcccggta
     1561 ccagtcgatc gtttcgcgca gccgctcttc cagcggccgg gcgggcgtaa agccgagctc
     1621 acggctggcg gcttcgccgg agcaggccca gttggtggcc agggcctcgc gcatcttgtc
     1681 gaggttgagg acatcggccc gtccgcggat ccgcgagagc ccctcgctga atccggcgac
     1741 gcaccaggcg accggcgctg gcagcgagat caccggcgcc cacggccgcc gcaccatcgg
     1801 ccggacgatc atgcccagtt cggcgtacgt gggatgctcg gcggcagtgg caaagtagat
     1861 tccctgcccg gcatggccgt tgttgccggc ggtcagccgc gacccgtgat cggcggcccg
     1921 caggagcagc tccacgaggt cggcaacgta aatgaacgag agggccggcg gatgcaagcc
     1981 gggcgagagg tggaaccggg cccagacgat gaattgcagc atgcggacga tctccgggtc
     2041 gcgcgggccg aatacgactc ccggccggac gatcgtcgag gggactttcg ccgcctcgcg
     2101 gcggacggcg ttttcgccgg ccagcttgct gcggccgtag tcggagtcgg gcgcgggcgg
     2161 atcggtttcg atcagaatct gcccgcgcgg cgcggggccg gcggccgaca gcgaagagac
     2221 atagatcagc cgcggcggct gggattgctc ggcgcaggcg gcggccaccg cggccgtgcc
     2281 ttctccgttg acccgcgaca gctcgccaaa ggtgagcgac tgcgtcaggc ccgccagatg
     2341 atagaccgct tcagcgcccg cgaccgccgc agccagctcg gccggttgat cgagggccgc
     2401 ggtgaccagc tcgacgtcga gcttccgcag ggcttcgtcg cgccggccgc cccgcaccag
     2461 gcaccgcacg cgctcgccgc ggcgaagtag cgcctcgacc aaatgccgtc cgatgaagcc
     2521 agtcgcgccg gtaaccagaa ccgtggacat cgcaggtgcc gatcaagatg ctggggaatc
     2581 ccgccaggtt cccaacttcg agtatgtctg caagcatcca tcgttccaag cgccggcggg
     2641 cgatgagcgt tgggcgaaac gggtgcagcg gtccgattgt gaggaatctg cggccagcgc
     2701 gggccaccgg cgaatgcggg ggtggcgcaa gaaaaaaccg cggacctccc cgctcggagg
     2761 tccgcggccg aaagtcggcg cgactccgcg ccgaaccgat aacgacttgc cctggcctat
     2821 ccgtttttca ggaccttgaa ctgcgaccct tccgacgtgc ggacgaggac cagcacgccc
     2881 ttatcgagcg aggcgttctt cgccgccgtc tcgaactcgg tgaccgactt gatcgtctgc
     2941 cgtccgacct ggacgaccgc catgcccggt tccaggcccg ccttcgcggc ggcgctgtct
     3001 tcctgcacgg cgacgatcac gacgccttca ttggccgtga cgcccagctg cttcgccacg
     3061 tcggcggtca gcggctcgac ttgaaggccc agccgatcga agttgcccga ctgcgactcc
     3121 gcggtgagcg gacgcgtcgc gagttcgcca tagttcgcgg gctgctcctt cagcgtcact
     3181 tgcagctcga cgcgcttgcc gtcgcggagc acggccaggg gcagcttgct gccgatcggc
     3241 gagcgagcgg ccatcgcctg gagctggcgg acgttactga tcttcgcgcc gccaaactcg
     3301 atcacgacat cctgcggctt cagaccggcg gccgcggccg gcgaatcgga ccggacctcg
     3361 gtgacgatcg ctccgaccgg cgtggccatg ccgagctgat cggccagctc gccgttcacc
     3421 ggctgaatgc cgatgcctag ataggcccgg tggactttgc cttccttgag gagctgcggc
     3481 gtgacccacc tggccacgtt gaccggcacg gcaaagccga cgccttcgaa gtggcccgtg
     3541 gacgagctga tcgccgtgtt gatgccgacg acttcgccgt tgagattgac gagcggcccg
     3601 ccgctgttgc ccggattgat cgcggcgtcg gtctggatga actcctcgtg cttcatcatg
     3661 ccgatgtcgc ggcccttggc gctgacgatg cccgccgtga ccgtgtcggt caggccaaag
     3721 ggctggccga cggccagcac ccaatcgccg acccgtagcg aatcgctgtc gcccagtttg
     3781 gcatagggca gcggcccacc ggccttgatt ttgacgaccg ccaggtcggt gcccggatcg
     3841 gtgacgacct tttcggcgac gaactcgcgg ccgtcgttca ggcggacggt cactttgccg
     3901 tcgcccgcca cgacgtggtt gttggtaaga accacgcccg cggcgtcgat gatcacgccc
     3961 gagcctgagc tgcggcggcc cggcatctgc ggcgcgtcgt agtcaaacga gtcgccgtcg
     4021 ggaatctgct cgaagaatcg cttgagcagc ggatcgagat cgccgaactc cttgggcagg
     4081 ttcggcgtat ttggccgccc gccgcgcggc gcgcggagct cgcgcttcac gacttgcggc
     4141 tgggtttcat tgcggatcga aacgacggcg ggaatcacct ggtccgccga aaaacgaaac
     4201 gcctcggaca aggcgttagc ctggttcaaa ccctgagtgg gggcgggctt gctgaccgcg
     4261 gtcgaatcgg ccgtgagcag cccggccggc gaaaccgcgt agatcgcggc cgccagagcg
     4321 acgacgagcg aagctgccag cgtcacccgc cggggagctg tgcgatacaa actgtccatc
     4381 ccgatactct cctttctgcg tccggcggca gtcggcgcgc cggttcgtac aagaattgcc
     4441 tcgaatacct gttcctactg aaattgttgc gcgctggttc gcgcagcttg cggaagattt
     4501 accggcagcc ccgcccggcg attcaagtgc ttgcgctgca aggaattcgg gcgagcgcta
     4561 caaaacggca aacatcgcgg ctgtcaaaat gacaacccgc cgccggcaaa accttagcat
     4621 ttgcagccag cgtgccaatt cagcaaggtt acgctcgcgt catacttggc gcagccgcct
     4681 ggcccgcgaa attcagaatc tgccggttta cttcggcggg ctcttcgtgg ggaagccagt
     4741 gcgtggcatt ctcatagaat tgcagctggc cctgatcgca aagttggagg ctccgctcgg
     4801 cggcgacggg cagcacgaat ttgtcctgct tgccccagag gatcagggtg ggcggaagga
     4861 tccgcttgct tcgcggcggc cgcggcggag cccgaaacgg cgcacggtac cagttgagca
     4921 tcgccaagat ggcccccggc tgcgaccagg cgcgccggta ttcgacgaag tcggcgtcgg
     4981 tgaacgcccc aggccggctc gagtttcgga ggccgcgaac caggtcccgc cagtcattgc
     5041 gcgcgaggct ccactcgaac agccaaggca cctggagggc gaagacgtac cagcttcgca
     5101 gcgtctgccg cggattggta aggatcagcc tccgcataac cagcgggtgc ggcacgttga
     5161 tgatcaccag ccgctcgacc agctcgggat ggttcgccgc cagccaccag gcgacgacgc
     5221 cgccccagtc gtgaccgacg acgagggccc gccgtcgtcc ggcggcggca atgagcccgg
     5281 cggcatcggc cgccagttgg tcgaggcagt agtcctgcac ccgcggcggc ttatcgctca
     5341 ggttgaagcc ccgctggtcg gccgcccaaa cgcaatagcc tgcggcgacc agcgccggaa
     5401 tctggtgacg ccagccatac cagaattccg gaaacccgtg cagcaacagc accagcgggc
     5461 catcggccgg cccggcctgc gcgacattca gatgaatgcc attggttgcg atccgctggc
     5521 aggtgacgcc gtccaagggt tatctccgct ggtctccgta tccgaattcg ccagaattcg
     5581 gtctttccac agcaccgaat tctggcgaat tcggctacgg aaagttcgtg cccctcgctc
     5641 acgcttttgg ttggtaggcc tggcagccgg ccgcttcgat cgtgtcgatc agccgctgcc
     5701 gatcatccgg cggcaggtcg tgacactcga ctttgacgtg ctgccaggtc caggttgcct
     5761 cgaacctgcc ctcacccgcc cgagcgatga gcaggcccga ctgctcatca tgctgcggaa
     5821 acgccgcctt gagccggtcg ataatggccc gcacgtcgag gtcgatcagc cccgcgactt
     5881 cctcgcccca gacgatctcg cgggcgactt cggccggcga gagccgcagc tgggggccgg
     5941 gattccaaaa gagcagcgtt tcactcatcg ccgatccgcg ctaggaaagg gttatattcg
     6001 tgggcgacac cgacgccggc agcgccggcc caccgtgatt ctaccgctgg gagtcaactg
     6061 cgtgacggag ccgatcatca gcgtttccgg actgcggggg atcgtcggcg agagcctgac
     6121 gccgccggta gccgcccgct acgtggccgc gctggcgagg gcgctgccgc ccgggccgct
     6181 ggtcgtcagc cgcgacggcc gcacgagcgg accgatgctg gccgagatcg tgtgcggcac
     6241 gctggccgcc tgcggccgca atgtgctcta cgccaacgtc gccgcgacgc cgacgctcgg
     6301 cgttctcgtg cggcagcatg aggcggccgg cggcgtgcag atttccgcca gccacaaccc
     6361 agccccctac aacggcctca agctcttcaa cgccgtgggg cgggtcctgc cggcggccgc
     6421 cgggcagccg gtgttggccg cgtatcgcga atcgtccgct ccctgggcgg cgttcgacaa
     6481 gctcggttcg gtttcgacga ttgccgatac gaccggcgag cacttgcggc tgatcctggc
     6541 ggcggtcgat gccgccgcca tccgccgccg cagctttcgc gtcctgctcg acagcaatgc
     6601 cggagcaggc agcctcctcg gcctgcaact gctcgaagcc cttggctgcc aggtgtcgat
     6661 cctcggccag cagccgacgg ggcagttcct gcatccgccc gagccgacgg cggagaacct
     6721 cgccggcgtc ggggagcaag tgaccgcggc caaggccgat gtcggcttct gccaggaccc
     6781 cgacgcggac cggctggcaa ttatcgacga gaacgggcgt tatctgggcg aagagtacac
     6841 gctggcgatc tgcctcgacc atgccctgcg ccagcgaaag gggccggtcg ttacgaactg
     6901 cgccacgagc cggatgtcgg aggacctggc gcagatttac ggcgtgccat ttcaccgcgc
     6961 ggcggtcggc gaggcgaacg tcgtcgatca gatgctcgcc cagcgcgcgg tctttggcgg
     7021 agaaggctcg ggcgggccca tcgatccgcg ggtggggctg gtccgcgaca gttttgtggg
     7081 gatggcgctg gtcctggccg cgatggccac ccgcaatcgg ccggtcagcg agttggccgc
     7141 ggagctgccg cggtacgaga ttgtcaagcg gacggtaaac ctcgccgtgg aaaagctgct
     7201 cgcggcgctg gccgccgtgg agcagcgttt cgccgacgcc aaggccgacc ggctcgacgg
     7261 tctgcggctt gactggccca gccgctggct gatcgtccgc ggcagcaaca ccgagccgat
     7321 cgtgcggatc attgccgagg ccccgacggc cgtcgcggcg gaagaaatgg cggccgctgc
     7381 ttgcgacgcg gtggcgggcg tctaaagtcg tagggcaagc tgccagcttg ccccatctcc
     7441 gcagttatgc aacaagctgg cagcttgttg ctacgaggag ccgcctgtgg aacgcgcgat
     7501 cagcttcgcc ggttactttg tgatgatcgg cgtggcctgg tgcttgagcg gcgacaagcg
     7561 gcggtttccc tggcgggtcg tgatcgtcgg tacgctgctc cagttcgcct tggcggtggt
     7621 gattctcgac acgcggccgg ggcgaatcgc gttcgccgcg atcggcgacg cctttcgctg
     7681 ggtctcggac ctgaccgatg tggggagcaa gttcgtgttc ggcgaccgct tcctggagca
     7741 tccgtttgcc ttcaaggtgc tgccgacgat catctttttt tcgtcgctgg cggcgattct
     7801 gtattacctg ggcatcatgc agctcgtcgt caaaggcatg gccgtcgtga tgcagcgctt
     7861 catgggcgtg tcgggggccg aagcgctgtc ggccagcgcc aacgtcttcc tgggccagac
     7921 cgaagcgccg ctcctggtga agccttacat cgccgggatg acgctgtcgg agttgaacgc
     7981 cgtgatgatc ggcggctttg ccaacatctc gggcagcctg atcgcagcgt tcagcgccaa
     8041 ggagttcggc ctcgaccccg gccacctggt gacggcctcc gtgatttccg cgccggccag
     8101 cctgctgatc gccaaggtgc tcgtgccaga gaccgagcag ccggcgacgc tcggcaaggt
     8161 caagatggaa gcgccgccgc agggcgcgaa cctgatcgag gcggcggcga tcggtgcggc
     8221 cgacggcgtg aagctggcta tcaacgtggc cgcgatgctg atcgctttca tcgccctgat
     8281 cgccatgctc gacgcagccg tgcaggcgat cggcattggc cttgcccgct ccaccggctg
     8341 gacggcggcc gccgggtgga ccctctccgg cctgctcggc tacctcttca gcccgttcgc
     8401 ctggatcatg ggcatcgaga cgcgggactg cctgcgggcc ggcgagctgc tgggcaaaaa
     8461 aatgtttgcc aacgaatttg tggcttacac gcaactggcg aaatggaagg aggagagctc
     8521 cagcgtgcag ctcagcccgc ggaccatcac gatcatgacc tacgccctgt gcggcttcgc
     8581 gaatttttcg tcgatcggca tccagatcgg cggcctcggc ggcatggccc ccgagcggca
     8641 gtcggacatc gcccgcctgg gcctgcgggc catgctcggc gggacgatcg tcaatttcat
     8701 gatgggctgc atcgccggcg tcttggttta accacgctgt tttcgcgagc cgcgccgcgg
     8761 ctccttagcc tgctggggct gaaacgacgc aaacgccgcc ggcaagagct cgcggaggtt
     8821 cgcttcgacg attcgcttgg gctggtccgc atcgaccagc aggatgggca gctgcggcgc
     8881 aaattcggcc agcacctgcc ggcaagctcc gcacggcgtg acgccgccgc tggcggcaat
     8941 cgccagcaac tggaactccg tcgcacccgc cgcgacggct gcgaaggtcg ccgctcgttc
     9001 cgcacaaatc gtcaagccat acgacacgtt ctcgacgttg cagccgacaa acatccgccc
     9061 atccgccgcg aggatcgccg ctcccaccgc gaacttcgag tacctggcat acgcccgccg
     9121 acgaacctcc agcgccgccg caatcaaccg ctcccgctcg ctcgccgcca tcagcatcct
     9181 caatccaaaa agcaacccgc tgtttttcga actctccgaa aaaccgcggg tcaccagtgg
     9241 tcaggggcgg agttgaaccg ccgacacatg gattttcagt ccattgctct accaactgag
     9301 ctacctgacc atttgccact tttcccagtg ttttctgggt tttttgtcaa gtctcggttt
     9361 cgacctccaa gtgcttgaca ccacttttga caccagttag gcttctgttc gtcaaaagtg
     9421 acacccgccg tattcagcag cgggtgtcat gacaccacca accgctttga cctggaggtg
     9481 atgccgtgac cgattctacc aagcgccgtt ccgctcgcaa accgggttct gacgagaagc
     9541 tcaactggcg aattgcctgg ggcctgccac tctcgtaccc gctctttcct caccagaatg
     9601 gccgatgggc caaaaaggtt cgcgggaaac tttcatactt cggcaaagtc gccgacgacc
     9661 ccaaaggggc caaggccctg gagctgtggg ccgagcagaa ggacgacctg ctggccggcc
     9721 gcacgccgcg gaccaaaacc gccgggacga cggtcgccga gctgtgcgac catttcatgc
     9781 aggcgaagga cgacctgctg gagaccggcg agatcacccg ccagacgcgg acggactacg
     9841 aacgcacgac cacccgcatc gtggccagat tcggcaagac gcggaccgtc gcggacctga
     9901 ctcccgacga cttcaaggat ctacgatcca gcctggccaa gaccctcggg cacgtcgcgc
     9961 tgggcaacga gattcagcgc gtccgcgtcg tgttcaaaca cggctacgac actgggctga
    10021 tcgaccaacc gatgcgatac gggccgctgt tcaaacgccc cagcaaaaag gccctgcggc
    10081 tcgaacgggc cgagaagggc cagcggatga tcgaggccgc ggacatgcgg cggctgatcg
    10141 aggcggctga tgtccagatg cgtgcgatga tctttctggc ggtcaactgt ggtttcggca
    10201 acagcgactg cggcacgctt ccgctggcat cactcgacct ggccggcggc tggctcgact
    10261 atcaccggcc gaaaaccggc atcgaccgcc gttgtccgct gtggcccgag actgtcgctg
    10321 tgctcgcgaa ggcgattgag aagcggccgg cgcccaagtc gcccgacgcg gagcccctgg
    10381 tttttgtgac gaagtacggc gctccgtggg cgaaggagac tttcgacaac ccggtgacca
    10441 aagaattccg caaactgctc gacgatctgc agctgcatcg agcggggctc gggttttaca
    10501 cgctgcggca cgtcttccgc acaatcgccg acggctcgcg ggaccagccg gctatcaaca
    10561 gcatcatggg acacgccgac gcaagcatgg ccgccgcgta tcgcgagcgg atcgacgatg
    10621 cccggctgcg ggccgtagct gatcacgttc gcgcgtggct gtttcccgaa gtcgtggcga
    10681 aagagaagga ggcagctgcg gtggcattgc aggccaaggc ggagcgggag ccgcgggctg
    10741 cgaagcaagc taagccgaac cgccgtgccc ggccgcaagc ggtggctgac gtctcgttct
    10801 tgcgggtggt cgggtagaat cctcattcat cgggacggcc gccgggttcg aaaccgcgcg
    10861 gccaagcagc cccaggcggt caagcggctt ctctgtgctc gccgctggcc gccgcgggct
    10921 ggcccgtccc cggagcacag aacatggatg ccgatcggct ggagcggtgc aatccaaatc
    10981 tcccgcgagt cgaggccgac atcgccgccg ggaaactggc
//
LOCUS       DASRJB010000884         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2784600, whole genome shotgun sequence.
ACCESSION   DASRJB010000884 DASRJB010000000
VERSION     DASRJB010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784600"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1097)
                     /locus_tag="VFB80_23280"
     CDS             complement(81..1097)
                     /locus_tag="VFB80_23280"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZN36779.1"
                     /translation="MSPDRRTFLQLAALASSAASLEANAQEAKRFAIAIHGGAGKSAD
                     RMPPEEVKAVEASLGQALDIGVKVLQEGGASLDAVEQVLRFLEDDPLFNAGRGAVFNA
                     AGEHELDASIMDGKTKACGAVAAVRTVRHPISLSRLVMEKTRHVLLTSDGAEKFADEM
                     RVERVENKWFDTDKQRRDWERSRAADAKKQAATGHSPLTTDSAHYGTVGCVALDAAGN
                     LAAGTSTGGISNKKYGRVGDSPIVGAGTYADNATCAVSCTGTGEQFIRNAVAYDLSAR
                     LKYLKQPLAEAVNEVLHKTLRPSDGGLIALGADGTIVMDYSTGGMARAAADASGRREV
                     KIGR"
     gene            complement(1234..2181)
                     /locus_tag="VFB80_23285"
     CDS             complement(1234..2181)
                     /locus_tag="VFB80_23285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN36780.1"
                     /translation="MTVGPDSAGQRLDAFLAQVLPKYSRVQLRKVIGGGGVTVNGQGT
                     KVAHRLSAGDRILVVLPPMNTAGPHPEEIPLEILYEDEHIIAVNKPPGMVVHPARGHW
                     SGTLASALSFHFRQLSSAGGPTRPGIVHRLDRDTSGVMVVAKTDPMHYALAAQFENRT
                     TEKEYFAITAGVPDRDRDIIEQPIGIHPQQREKMAIRPGHSTSRGATTFYEVVERFAG
                     FAAVRVLPKTGRTHQIRVHLAHIGCPVLCDKLYGGRSSVSRRELVRGGDETVVLARQA
                     LHAQRIKLAHPATGEPIELIAPLPADISHVLNVLRQPGK"
     gene            complement(2225..3127)
                     /locus_tag="VFB80_23290"
     CDS             complement(2225..3127)
                     /locus_tag="VFB80_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN36781.1"
                     /translation="MKAERRHELQQNELADWLGEKIEALKPQATQIAIVVLAVVVLVV
                     AGTWYFGTEDHASARSWSDFFGAMNQPRDSEKLLEKLSIDKPGTAPGMWAMMTLADTS
                     AEQGGRAMFTDRPEAQKLLNKAETSYKAVEAAATHDPLLQSRARLGLAKVYESLCKPE
                     EALKYYEQVAASQKDTAIGKSAAADAARMKNKEQVDLLAWFAVQTPKRPAPIPGFGGG
                     IPGLPNDLPERPDISVPSITPPSGLGLDNIGTGVPTVPTPEFPAPGTTTPPAPTTPQS
                     KTGDTNPAEPKTEAPKADSPKSEP"
     gene            complement(3282..4001)
                     /locus_tag="VFB80_23295"
     CDS             complement(3282..4001)
                     /locus_tag="VFB80_23295"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN36782.1"
                     /translation="MTFRSRWLVGYLAAALALVIRLWVGTLRVRKVSADGREHPTDPR
                     QQRYIYIFWHEGLLAPLTTHAKVRVLISQHTDGELIAQICRRLGIGVIRGSTARGGSQ
                     ALMEMIRSSGEDHLGITPDGPRGPRRELKPGAVMVASQSGLPIVPVGIAFVRAWRAGS
                     WDRFALPLPFSTLIGVMGEPIEVPADLDRGGLKHWTRHVEEQMGALTEAAENWADRVR
                     REGRRAQPPELAHRTRLSRSA"
BASE COUNT          658 a         1365 c         1362 g          780 t
ORIGIN      
        1 ttcagaagat cgacagggac ggggctgtcg ttctgggttc gtccttctgc attctgaatt
       61 ctgcatttga ataccgggcc ttaccgccct atcttcacct cgcggcggcc gctggcgtcc
      121 gccgcggcgc gagccatgcc gccggtggaa tagtccatga cgatcgtccc gtccgcgccc
      181 aaggcgatca gcccgccgtc ggacgggcgg agcgtcttgt gcaggacctc attcaccgcc
      241 tctgccagcg gctgcttcag gtacttcagg cgggccgaga ggtcgtaggc gactgcattc
      301 cggatgaact gttctccggt gccggtgcag ctgaccgcgc aggtggcgtt gtcggcatag
      361 gtgcccgcgc cgacgatcgg cgaatccccg acgcggccat acttcttgtt cgagatgccg
      421 ccggtcgaag tgccggccgc gaggttgcct gcggcgtcga gggccacgca gccgacggtg
      481 ccgtaatggg cggagtcggt ggtgagtggt gagtggccag tggcagcttg tttcttggca
      541 tcggcggccc gcgagcgttc ccagtcgcgg cgctgcttgt cggtgtcgaa ccatttgttc
      601 tcgacgcgct ccacgcgcat ttcgtcggca aacttctccg cgccgtcgct ggtcaagagg
      661 acgtggcggg tcttttccat caccagccgc gagagcgaga tcgggtgccg cacggtgcgg
      721 acggcggcca ccgcgccgca ggccttggtc ttgccgtcca tgatcgaggc atccagctca
      781 tgttcgccgg cggcgttgaa cacggcgccg cggccggcgt tgaacagcgg atcgtcctcc
      841 aggaagcgga gcacctgctc caccgcgtcc agactcgcgc cgccctcctg gagaaccttc
      901 acgccgatat cgagcgcctg gcctagcgag gcctcgaccg ccttcacttc ttcgggcggc
      961 atcctgtccg ccgacttccc cgcgccgccg tggatggcga tcgcgaatcg ctttgcctct
     1021 tgcgcatttg cctcgagact agccgcgctc gacgccagtg cggccaactg aagaaacgtg
     1081 cggcgatctg gcgacatgga agcatctcct gactcacctg aacggaggcg gatcgaacag
     1141 aaggtaacca aggaaacgaa gagagaaaag gagaataatt ctcttgcgac cgtttctctc
     1201 tgaccgtcct tcgttttctt cgtttccttc tgttcatttc cctggctgcc tcagcacatt
     1261 caaaacgtgt gaaatatccg ccggcagcgg tgcaatgagc tcgatcggtt cgcccgtcgc
     1321 cggatgggcg agcttgatcc gctgggcgtg gagcgcctgc cgcgcgagaa cgacggtctc
     1381 gtcgccgccg cggacgagtt cgcgccgact caccgacgag cggccaccgt agagcttgtc
     1441 acacagcacc gggcagccga tgtgggccag gtgcacgcga atctggtgcg tccggccggt
     1501 tttcggcagc actcgcacgg cggcaaagcc ggcgaaccgc tcgaccacct catagaacgt
     1561 cgtcgccccg cggctagtcg agtggcccgg acggatggcc atcttctccc gctgctgcgg
     1621 atggatgccg atcggctgct cgatgatgtc gcggtcgcgg tcgggtacgc cggccgtgat
     1681 cgcaaaatac tccttctcgg tcgtgcgatt ctcaaactgc gccgccaggg cgtagtgcat
     1741 cgggtcggtc ttggcgacga ccatcactcc gctggtgtcg cggtcgagcc ggtgcacgat
     1801 gcccggccgc gtcggaccgc cggccgagct gagctgccgg aagtgaaacg acagcgcgct
     1861 ggccagcgtt cccgaccagt gcccgcgggc cggatgcacg accatgcccg gcggcttgtt
     1921 gaccgcgata atgtgctcgt cctcgtagag aatttcgagc ggaatctctt cggggtgcgg
     1981 gccggccgtg ttcatcggcg gcagcacgac cagaatccgg tcgcccgcgc tcagccgatg
     2041 ggcgaccttc gttccctggc cgttgacggt cacgccgccg ccgccgatca ctttgcgcag
     2101 ctgaacgcgg ctgtatttgg gcagcacctg cgccaggaag gcatccagcc gctggcccgc
     2161 cgaatcgggt ccgacggtca actcgagtgg ctggtcaggt ggcagagggt tcagggttcg
     2221 gggttcaggg ttcagacttg ggtgagtccg ctttgggcgc ttcggtcttc ggctcggccg
     2281 gattggtgtc gcccgtcttg gactgaggcg ttgtgggcgc gggcggagtc gtcgtgcccg
     2341 gcgcggggaa ctccggcgtc ggaaccgtcg ggactccggt gccaatattg tccaggccca
     2401 ggccgctggg cggcgtaatg ctgggcacgc tgatatccgg tcgttcaggc aagtcgttgg
     2461 gcaggccggg aatgccgccg ccaaagcccg gaatcggcgc aggacgcttg ggcgtctgca
     2521 ccgcgaacca ggccagcaga tcgacttgct ccttgttctt catgcgagcg gcatcggcgg
     2581 cggcggactt gccgatcgcc gtgtccttct ggctggccgc gacttgctcg tagtacttca
     2641 gcgcctcctc gggcttgcac agcgactcat aaaccttggc cagacccagc cgtgcccgcg
     2701 attgcaggag cggatcgtgg gtcgccgccg cctcgacggc cttgtaggac gtctcggcct
     2761 tgttcagcag cttctgggcc tcgggccggt cggtgaacat cgcccggccg ccctgctcgg
     2821 cgctggtgtc ggccagcgtc atcatggccc acatccccgg agcagtgccc ggcttatcga
     2881 tgctcaactt ctcgagcagc ttctcgctgt cccgcggctg attcatcgcg ccgaagaagt
     2941 cgctccaact cctggccgag gcatgatcct cggtgccgaa ataccaggtt cccgcgacca
     3001 ccaagacaac gaccgccagc acgacgatcg cgatttgcgt tgcctgtggc tttaaggcct
     3061 cgatcttttc gcccagccaa tcggccagtt cgttctgctg cagttcgtga cggcgttcgg
     3121 ctttcatggg cgtatggggc agtcgttgac gcggcgagca actttcgcta cagcgcgcaa
     3181 gtataggagc ggtcagaaga tagcgtcaag gcgggtgttt agtgtagacc tcaccctccg
     3241 cgtgaggacg tccgatcgcg gagcgagcgg actgcacacc attacgccga gcgcgagagg
     3301 cgtgttctgt gagccaattc gggcggctgg gctcgccgcc cttcgcgccg cacgcggtcg
     3361 gcccagttct cggctgcctc ggtcagcgcg cccatctgct cttcgacatg cctcgtccag
     3421 tgtttcaggc cgccgcggtc caggtcggca ggtacctcga tcggctcgcc catcacgccg
     3481 atcaacgtcg aaaagggcag cggcagggcg aagcggtccc agctgcccgc tcgccacgcc
     3541 cgaacaaagg caataccaac cggcacgatc ggcaggccgc tctgcgacgc gaccatcacg
     3601 gcccccggtt tgagctcgcg gcggggcccg cgcggcccgt cgggcgtgat gcccaggtgg
     3661 tcctcgccgc tgctgcggat catctccatc agcgcttggc tgccgccgcg ggccgtcgag
     3721 ccccgaatca cgccgattcc tagccgccgg caaatctggg cgatcagttc gccgtcggtg
     3781 tgctgactga tcagaacgcg gaccttggcg tgcgtggtca gcggcgcgag caatccctcg
     3841 tgccagaaaa tgtagatgta tcgttgctgg cgcgggtcgg tcgggtgctc gcggccatcg
     3901 gcgctcacct tgcgcacgcg gagcgtgccg acccacaggc ggatcaccag cgccagcgcc
     3961 gcggcgagat aaccaacaag ccagcggctg cgaaacgtca tgcgatgaaa ctcggaaatg
     4021 acagcggcaa attgtcggcg ccacgaatgg tgccaagaag caaccaacgc ggcaaggcga
     4081 gaattgcccc gtctgccaag attttgccgc ttgctcttcc cccggcggcg ggattggtca
     4141 taataaaagt gcatcccaag ctgat
//
LOCUS       DASRJB010000885         9077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3366304, whole genome shotgun sequence.
ACCESSION   DASRJB010000885 DASRJB010000000
VERSION     DASRJB010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3366304"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..705)
                     /locus_tag="VFB80_23300"
     CDS             complement(88..705)
                     /locus_tag="VFB80_23300"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36783.1"
                     /translation="MDKDSTSQLQTLLDRLRQGDRQARREFLEQVCERLRRLAAKILS
                     GSFPNLQPRHEVDSVVHETWLRLMQALDKADPPTVADFFRLAAHKIRQVLLDMADRQR
                     RIDQRETFLGLADSQAAGPDSPHAEPGNQTYDGAKLALWTELHNKVGLLPEDERTVFE
                     MHYYLGLPQAEIAKILDLHPRKVSYLWIAATENLAGEMSALSPHT"
     gene            877..2013
                     /locus_tag="VFB80_23305"
     CDS             877..2013
                     /locus_tag="VFB80_23305"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN36784.1"
                     /translation="MIWKPVGVGAALLAAAGSAWAVWFNWPAAAREMRLPGTVETQEV
                     RLSSRVGGRVSKVLVKESQLVEAGQPIALLEMPELDTQRAQLVAQKEAAEAVLARLEK
                     GSREEEKAAAKAAVDSAQARLARMTKGYRTEEKEQARSELQAIDAQVQNALAELKRER
                     NLLTTKASTAALYDAALSRYNRLIAEQSAAAAKARMMESGYRDEEVQEAQAELNRLQA
                     NYDLIIAGSREEDKQEQRALVAKLQAQIDEIDVKRAERTVYAPEKAVVEIVMVRPGDI
                     AQANQPVALVLRADDLWVKAYISEVDLGKIKLGQKVEVTCDSFPGKRFEGEITYIASA
                     AEFTPRNVQTIDERRHQVFGFRVRVADSQGVFKSGMAADVWLPK"
     gene            2092..3039
                     /locus_tag="VFB80_23310"
     CDS             2092..3039
                     /locus_tag="VFB80_23310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN36785.1"
                     /translation="MSSANEPVIDIRDLTIRFGSFTAVDRVSLQVERGEVFGLLGPNG
                     SGKTTLIRALCGLLPLAGGGATVLGHDVARDAERIRQSIGYMSQKFSLYADLSVQENM
                     DFYSGIYGLRNSEAKQRQAELVELTGLEPYLGRWAGKLSGGWKQRLALVCALLHRPEL
                     VFLDEPTAGVDPVARRELWDLLFQLAGQGITLLVTTHYMDEAERCGRVGYLHLGTLLA
                     LDTPSALKRLPEVHPPGFRRLEIVTPDTAELLVRLKHRPTVQEATIFGQSIHALVRDD
                     DTLADIAGPDVIIRPAAANLEDVFVTIARNRQRELAQAG"
     gene            3069..4181
                     /locus_tag="VFB80_23315"
     CDS             3069..4181
                     /locus_tag="VFB80_23315"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN36786.1"
                     /translation="MNGNTFLRTFSVARKELLHILRDPQTLFFTLFVPVMELFMLGYA
                     IDTNVRNIRTVVVDYSGTQESRQLIDQFRNSGDFRIVANAYSDQVASRMIVAGKARIG
                     IVIPENYTRRLEAGQTARILVLVDGTESSIAGEAVNVSNAITLRESLVRILGNKPLPV
                     ESQPRVLFNPDTRSANFFIPGLMVVMCQMMSTMLSANAIVREKELGTLEQLYMTPIRR
                     GELILGKLSPYIVLTMLEFCFIAFLMWLVFQVPIHGSFLTLLMVSFPFFLTNLGLGLW
                     ISTKAATREASGQMAMGTFLPSIFLSGYVFPIDSMPALLQPVSQCFHTTWMIDASRSV
                     ILRGGGWPELWPHATVLWGMALAALVFGSLQVQKRV"
     gene            complement(4187..5770)
                     /locus_tag="VFB80_23320"
     CDS             complement(4187..5770)
                     /locus_tag="VFB80_23320"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZN36787.1"
                     /translation="MSDELIHRYLDDRSALSEGELDELIAHLRAEPARAAALREQLLL
                     DDLLAQKLAVDRQNFPAQVDQRIADFQRGQEELDAQVTELRQLAEAEIDRPRTWSGTS
                     PWVKYVLALSAAVLIGGVFFASQWLPAQPYTVLKVSKVEGNVTFQQGGQSSAAAVHAA
                     LLSGQQVNVPAGGSIDLVYQDATTVWLGGDAAVTFDTDRATGAKRVRIDRGELIASVA
                     PQAAGPMVFTTPHAVATVRGTKLRLAVTETETLLDVTKGLVDFQRLGDQNLQQVAASQ
                     SGIATSEAFFPVQALAWPASREGVVYLLSSLEAADDLPYTAVRIPDTNNYLLTELLPR
                     GAAQLRPGTLQWELSGGHLYSDEAGQDVLPQLTGRDELTLEVIFQPAASVATSPARIV
                     ALADDGESANLALSQEGSDLVFSLRTEDETIKPLRFSLLVANEPLESVRPKHIAVTHR
                     AGELVAYRSGAEVARSRLAGGTLAEWRGGPLTIGADSRGQRVWRGRVLAIVLSSRCLS
                     VAEIARSEASFRLLSAERK"
     gene            complement(5782..6312)
                     /locus_tag="VFB80_23325"
     CDS             complement(5782..6312)
                     /locus_tag="VFB80_23325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN36788.1"
                     /translation="MDERPPALDFDSIVRETQAHIRAYIAGMGVPRHDVDDIAQDVYV
                     ELYRFFDRIPPEVPPKQWLKGIARNLSLNYFRRTSRRQRLQREALVEIFLRAEQETAP
                     AVSEGPVRRALDGCYEKLAPDSRRLLSLRYEQELPSQTIAERLKSTAEAVRVALFRVR
                     ASLKQCISKSLASERG"
     gene            6959..7381
                     /locus_tag="VFB80_23330"
     CDS             6959..7381
                     /locus_tag="VFB80_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36789.1"
                     /translation="MAVVANAGAIAAAAAAAEQQRRQAEEEEEMTPYTQQDLNQDWEF
                     KILRSAAHWFKKPEYLRQCLEEEARAGWVLVEKFDDGRVRLKRPALARERDGKLDIDP
                     YRTTVGASPGAVALFTVVIALGIAMGILIAVALLAQIH"
     gene            complement(7495..8211)
                     /locus_tag="VFB80_23335"
     CDS             complement(7495..8211)
                     /locus_tag="VFB80_23335"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZN36790.1"
                     /translation="MSNAPIKSPAGKPTAGAADASSPTAGPLPRNRYVVYFAIALLGC
                     ALDLATKQSMFNWLGLPAAPFVHPNDPEAIARWRGEPGLPHRWWLFEGRLGIETSVNR
                     GALFGFGEGFWWLFATLSVVALVGIMVWLFAYKAAIDRWLTVALSFVSAGILGNLYDR
                     LGLWDSTGLEPAYRHGVRDWILFVWPESGLTMFNPWPNFNIADSLLVTGAIMLIVHAV
                     VWRGDGDRASGAGSRTASSA"
     gene            complement(8177..8551)
                     /locus_tag="VFB80_23340"
     CDS             complement(8177..8551)
                     /locus_tag="VFB80_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZN36791.1"
                     /translation="MLKKSESKEFKHMLLSLRARLRGDVTAMADAALRKTRSEASGDL
                     SSMPIHMADMGTDAFEQEFTLSLMEHDGSALEQIEAALVRIEDGHYGVCVECEAKIPK
                     ARLQVLPHTPHCVKCADKVASR"
BASE COUNT         1612 a         2987 c         2916 g         1562 t
ORIGIN      
        1 aaaccacggc tagtgatcgt tccgcgccgc cggctgtggt tcactggtgt gaaccacagc
       61 cacccgcccg aattctggcg aattcggcta cgtatgcggt gacagggcgc tcatttcccc
      121 agccaggttc tccgtggcgg cgatccacag ataactgact ttgcggggat gcaggtccag
      181 tatcttggca atctcggcct gcggcaggcc caggtaatag tgcatctcga acaccgtccg
      241 ctcatcctcg ggcaggaggc caaccttgtt gtgcagctcc gtccacagcg ccagcttggc
      301 cccgtcgtag gtctggttgc ccggctcggc gtgcggcgaa tctggccccg ccgcctgcga
      361 atcggccagg cccaggaacg tctcccgctg atcgatccgc cgctgccggt cggccatgtc
      421 gagcagcacc tgccgaatct tatgcgccgc gaggcgaaaa aaatcggcca ccgtcggcgg
      481 atcggccttg tctagagcct gcatcagccg cagccacgtc tcgtgaacga cgctatcgac
      541 ctcgtgccgg ggttgcaggt tcgggaagga gcccgaaaga atcttggcgg ccagtcgccg
      601 caggcgctcg catacttgtt cgagaaactc gcggcgggcc tggcgatccc cctgacggag
      661 gcggtcgagc agcgtctgca gctggctcgt gctatccttg tccatagcga gtccaggcgc
      721 aactggttga cagccaacca gttgcggcaa ttcgccaact tttcgctcac cgccgtgtct
      781 gaaaccccca tccgcgctgc tgagcttagt aggagcaggc tgaggccgca agcaagcggc
      841 cgagcgcttg ttgccacgat gagcgagagg tgggccatga tctggaaacc ggtcggcgtg
      901 ggggcagcgc tcttggctgc ggccggctcg gcgtgggcgg tctggttcaa ctggccggct
      961 gcggcccgcg agatgcgcct gcccggcacg gtggagacgc aggaagtccg gctcagctcg
     1021 cgcgtcggcg gccgggtgtc caaggtgctg gtcaaggaat cgcagctcgt ggaggccggg
     1081 cagccgatcg ccctgctgga gatgccggag ctcgataccc agcgggcgca gctcgtggct
     1141 cagaaggaag cggccgaggc ggtgctggcc agactcgaaa agggttcgcg cgaagaagag
     1201 aaggccgcgg ccaaggcggc ggtggactcg gcccaggccc ggctggcgcg gatgaccaag
     1261 ggctatcgca ccgaggagaa ggagcaggcc cgcagcgagt tgcaagctat cgacgctcag
     1321 gtgcagaacg cactggccga gctgaagcgc gagcggaacc tgctcaccac gaaggcctcg
     1381 accgccgcgc tgtacgacgc cgcgctgtcc cgctacaacc ggctgatcgc cgagcaaagc
     1441 gccgcggcgg cgaaggcgcg gatgatggaa tcgggctacc gcgacgagga agttcaggag
     1501 gcgcaggcgg agctgaaccg cctccaggcc aactacgacc tgatcatcgc cggcagccgt
     1561 gaagaggaca aacaggagca gcgggcgctg gtggcgaagc tccaggccca gatcgacgag
     1621 atcgacgtga agcgggccga gcggacggtc tatgccccgg agaaggcggt ggtcgagatc
     1681 gtgatggtcc gcccgggcga catcgcgcag gccaaccagc cggtggcgct ggtgctgcgg
     1741 gccgacgact tgtgggtgaa ggcctacatc tcggaggtgg acctgggcaa gatcaagctg
     1801 ggccagaagg tcgaagtcac ctgcgattcg tttcccggca agcggttcga gggggagatc
     1861 acctacatcg cctcggcggc cgagttcacg ccccgcaacg tgcagacgat cgacgaacgg
     1921 cggcaccagg tgttcggctt ccgggtccgc gtcgccgatt cgcagggcgt cttcaaatcc
     1981 ggcatggcgg cggatgtgtg gctgccgaag tagcatgagc gtcctcgctt gtgcagcgat
     2041 tactcgaatc cagcggtgag caagcgagga cgcttgctct acggtgtcgc catgtcctcc
     2101 gccaacgagc ccgtcatcga cattcgcgac ctgacgatcc gtttcggcag cttcacggcc
     2161 gtcgatcgcg tcagcctgca ggtcgagcgc ggcgaagtgt tcggtctgct cggccccaac
     2221 ggctcgggca aaacgacgct catccgggcg ctgtgcggcc tgttgccgct ggccggcggc
     2281 ggcgcgaccg tgctgggcca cgacgtcgcc cgcgacgccg agcgaatccg ccagtcgatc
     2341 ggctacatgt cgcagaagtt ctcgctctac gccgacctct ccgtgcagga gaacatggac
     2401 ttctactccg gcatctacgg tctgcgaaac tcggaagcca aacagcggca ggccgagctg
     2461 gtcgaactca ccggcctgga gccgtacctg ggccgctggg cgggcaagct ctccggcgga
     2521 tggaaacagc ggctggcgct cgtttgcgcc ctgctgcacc ggccggagct ggtgttcctc
     2581 gacgagccga cggccggcgt cgatccggtc gcccgccgcg agttgtggga cctgttgttt
     2641 caattggccg gccaaggcat caccctgctg gtcacgacgc actacatgga cgaggccgag
     2701 cgctgcggcc gcgtcggcta cctccatctc ggcacgctgc tggccctcga cacacccagc
     2761 gccctcaaac gcctgccgga agtccatccg cccggatttc gccgactcga aatcgtgacg
     2821 cccgatacgg ccgaactgct cgtgcggctg aagcaccgcc cgaccgtcca ggaggccaca
     2881 atcttcggcc aatcgatcca cgccctcgtc cgcgacgacg acacgctcgc cgacatcgcc
     2941 ggcccggacg tcatcatccg gccggcggcc gcaaacctcg aagacgtgtt tgtcaccatc
     3001 gcccgcaacc ggcaacggga gctggcccaa gcgggatgac aagtgacaaa tgacaagtga
     3061 caaatgacat gaacggcaac accttcctcc gcacgttctc cgtggcccgc aaagagctgc
     3121 tgcacatcct ccgtgatccg cagacgctgt tcttcacgct cttcgtgccg gtgatggagc
     3181 tgttcatgct gggctatgcc atcgacacca acgtccgcaa catccgcacc gtcgtcgtcg
     3241 attactccgg cacgcaggaa agccggcaat tgatcgatca gttcagaaac tcaggcgact
     3301 tccggatcgt ggccaacgcc tattccgacc aggtcgccag ccggatgatc gtcgccggca
     3361 aggcccgcat cggcatcgtc atcccggaga actacacgcg gcggctggag gccggccaga
     3421 ccgcccgcat tctggtcctt gtggacggca ccgagtcgag catcgccggc gaggcggtca
     3481 acgtcagcaa cgcgatcacg ctccgcgagt cgctcgtgcg gatcctcggc aacaagccgc
     3541 tgccggtcga atcccagccc cgcgtcctct tcaatcccga cactcgctcg gcgaactttt
     3601 ttatccccgg cctgatggtc gtgatgtgcc agatgatgtc cacgatgctc tcggccaacg
     3661 cgatcgtccg cgagaaagag ctgggcacgc tggagcagct ctacatgacg ccgatccgcc
     3721 gcggcgagct gatcctgggc aagctctcgc cctacatcgt gctgacgatg ctggagttct
     3781 gcttcatcgc ctttctgatg tggctcgtgt tccaggtgcc gattcacggc tcgttcctga
     3841 cgctgctgat ggtttcgttc ccgttcttcc tgacgaacct cggcctgggc ctgtggattt
     3901 cgaccaaggc cgccacccgc gaagcctcgg gccaaatggc gatgggcacg ttcctgccgt
     3961 cgatcttcct ctcgggctac gtcttcccga tcgattcgat gccggccctc ttgcagccgg
     4021 tgtcgcagtg cttccacacc acctggatga tcgacgcctc gcggagcgtc atcctccgcg
     4081 gcggcggctg gcccgagctc tggccccacg ccaccgtcct ctggggcatg gccctcgccg
     4141 cgctcgtctt cggctcgctc caggttcaga agcgagtgta gccgcgctac tttcgctcag
     4201 cggacagcag gcgaaagctc gcttcgctgc gggcgatctc ggcgacgctc aggcagcggc
     4261 tggacagcac gatcgccagg acgcggcccc gccaaacgcg ctgcccgcgg ctatccgcgc
     4321 cgatcgtcaa cgggccgccg cgccattcgg ccaacgtgcc gccggccagc cgacttctgg
     4381 caacttccgc gccgctgcga taggcgacca gttcgccggc gcggtgcgtg acggctatat
     4441 gcttgggccg aacggactcc agcggttcgt tcgccaccag cagcgaaaaa cgcagcggct
     4501 tgatcgtttc gtcctccgtg cgcagcgaga aaaccaggtc gctgccctcc tggctcagcg
     4561 ccaggttggc ggactcaccg tcgtcggcca gggcgacaat ccgcgcgggg cttgtcgcga
     4621 cggaggccgc gggctggaaa atgacctcga gcgtcagctc gtcgcggccg gtcagttgcg
     4681 gaagaacgtc ctggccggcc tcgtcggaat acaagtgccc gccgctcagt tcccactgca
     4741 gcgtgccggg ccgcagctga gctgcgccgc gcggcagcaa ttccgtaagg aggtagttgt
     4801 tcgtgtcggg aatgcgcacc gccgtgtacg gcagatcgtc ggcggcttcc agcgacgaca
     4861 gcagataaac gaccccctcg cggctggccg gccaggcgag cgcttgcacg ggaaaaaacg
     4921 cctcgctcgt cgcgatgccc gactggctcg ccgccacctg ctggaggttc tgatcgccca
     4981 gccgctggaa gtccaccagg ccctttgtta cgtcgagcaa cgtctcggtc tctgtcacgg
     5041 ccaatcggag cttcgtgccc cgcaccgtgg cgacggcgtg gggcgtcgtg aaaaccatcg
     5101 ggccggccgc ttgcggcgcg acgctggcga tcagttcgcc gcggtcgatc ctgacgcgct
     5161 tcgcgccggt cgcccgatcg gtatcgaacg tgacggccgc gtctccaccc agccagaccg
     5221 tcgtggcgtc ctggtagacg aggtcgatcg agccgccggc gggtacgttc acttgctggc
     5281 cgctgagcag cgcggcgtgc actgcggcag ccgacgactg gccaccctgc tggaaagtga
     5341 cattgccttc gaccttactg accttgagga cagtgtacgg ctgagccggc agccactgcg
     5401 acgcgaagaa cacgccgcca atcagcacgg cggccgacag ggccagcaca tatttgaccc
     5461 agggggaagt ccccgaccag gtccgcgggc gatcgatctc ggcctcggcc agttgccgca
     5521 attcggtgac ctgggcgtca agttcctcct ggccgcgctg gaaatcggcg attcgctggt
     5581 ccacctgggc cgggaaattc tgccggtcca ccgccagctt ctgggcgagc aaatcgtcca
     5641 aaagcagctg ctcacgcagg gccgccgccc gcgcgggctc agctcgcaag tgggcaatca
     5701 gctcgtccag ctcgccttcc gaaagcgccg agcggtcgtc gagatagcgg tgaatcagtt
     5761 cgtcggacat cgttcgcttg actatcctcg ttcactcgcc aggcttttgc tgatgcattg
     5821 cttcaaactc gcccgcaccc ggaaaagagc cacccgcacc gcttcggccg tcgatttcag
     5881 ccgctcggcg atcgtctggc tcggcagctc ctgctcgtag cgcagggaca ggagccgccg
     5941 gctgtcaggg gccaattttt cgtagcagcc gtcgagggcc cggcggaccg gcccctcgga
     6001 cacggccggg gcggtttcct gctcggcccg caggaaaatc tcgaccaggg cctcgcgctg
     6061 cagccgctgc cgccggctgg tgcggcggaa gtagttgagc gagaggttgc gggcgattcc
     6121 cttgagccac tgcttggggg ggacctccgg gggaatgcgg tcgaagaacc ggtacagctc
     6181 gacatagaca tcctgggcga tgtcatcgac gtcgtgccgc ggcactccca ttccggcgat
     6241 gtacgcgcgg atgtgggcct gcgtctcgcg gacgatcgag tcgaagtcga gtgcgggcgg
     6301 acgttcatcc atggtctgtc tagcaatctt atggtcggcc ggcggcgagt aattaccgcg
     6361 acggtctaat gcgcgcatac gcggaaaaag tctgcgcgag caggattcgc cgcaagtatt
     6421 tggtggacaa tgacttgcgt tcgattctgc gcgccgggca ttatgcgcat tatgcgcgca
     6481 agccgaaggc gtgaccgtgt tcagcccaat ggccgcttgg tagtgaaagt tggtaccggc
     6541 gcccttgcgg ggcacgcacg cagggtaagg ataattccgt gctcggcgag aattggtttc
     6601 gcggtgcctg cccggcgggg cagcgcaagt tgtgaatcta taatcattta ctaaaatgtt
     6661 gtttagattg aggcccggtt gcgttagaag cactagagac accagcggga attatcctta
     6721 cctgcttgcc gtctaccgga agggcggcag gaattccact gcgtgaaacg accaaattga
     6781 gaaagaacgc ggccgcgccc ccggcccttt ccggcggtgc tttgccacaa ttgtatagat
     6841 gcgccagcaa ggccatttat tgccgagatc ttttccgaat ttttagcttg cacggcaggc
     6901 cagagcccga tatccaccgc cgaactgcga atacccttcc gaagttgcga ggcccaagat
     6961 ggctgtcgtt gccaatgccg gggcgattgc ggctgccgct gccgcggcgg agcagcagcg
     7021 gcgtcaggcc gaagaggaag aagaaatgac tccctatacg cagcaggacc tgaaccagga
     7081 ctgggaattc aagatcctcc gctcggccgc ccactggttc aaaaagccgg agtacctccg
     7141 ccagtgcctg gaggaagagg cccgggccgg ctgggtgctc gtcgagaagt tcgacgacgg
     7201 ccgcgtacgg ctgaagcgtc cggccttggc ccgcgagcgc gacggcaagc tggacatcga
     7261 tccctaccgc acgaccgtgg gagcgtcgcc gggcgccgtc gcgctattca ccgtcgtcat
     7321 cgcgctgggc atcgccatgg gaattctgat cgcagtcgcc ttgctggcgc agattcactg
     7381 aggaacggcg agcgtcctgc gctgtaggga tcgccctctg tggcgatccg cgacgccgat
     7441 gtgctgagaa tgcccagagg gcggaatgcc acagagggca ttccctacag cgccttatgc
     7501 actgctcgcc gtccgactcc cggcccccga cgcccgatcc ccgtcccccc gccagacgac
     7561 cgcgtgcacg atcagcatga tcgcgccggt caccagcagg ctgtcggcga tgttgaagtt
     7621 gggccagggg ttgaacatcg tcagcccgct ctcgggccag acgaagagaa tccagtcgcg
     7681 cacgccgtgc cgatacgccg gctccagtcc ggtcgagtcc cacaggccca ggcggtcgta
     7741 gagattgccc agtattcccg cgctgacgaa gctcagggcc acggtcagcc agcggtcgat
     7801 agcggccttg taggcgaaca gccagaccat gattcccacg agggccacga ccgacagcgt
     7861 cgcgaacagc caccagaagc cctcgccgaa accgaacagc gccccgcggt tgacgctggt
     7921 ttcgatcccc agccgcccct cgaacagcca ccagcggtga ggcaagccag gctcgccgcg
     7981 ccagcgggcg attgcttcag gatcgttggg gtgcacgaac ggcgcggcgg gcagaccgag
     8041 ccagttgaac atcgactgct tggtggccag gtcgagggcg cagcccaaga gggcgatcgc
     8101 gaagtagacg acgtaccgat tgcggggcag cgggccagcg gtgggcgagc tagcgtccgc
     8161 cgcgccagcg gtcggcttac cggctggcga ctttatcggc gcatttgaca cagtgcggcg
     8221 tgtgcggcag gacttgcagc ctggccttgg ggatcttggc ttcgcactcg acgcagacac
     8281 catagtggcc gtcttcgatg cggacaagag cagcctcgat ctgctccagg gcgctgccgt
     8341 cgtgctccat caggctcagc gtgaactcct gctcgaaggc gtcggtgccc atgtcggcca
     8401 tgtggatcgg catgctggac aggtcgcccg aagcctccga tcgcgtcttc cgcagggccg
     8461 cgtcggccat cgccgtgacg tcgccccgca gccgggcccg cagcgacagc agcatgtgct
     8521 tgaactcctt ggactcgctc ttcttcagca ttatccgctc caatccgccc acgaacccga
     8581 tgaggaaagg tgcttctcga caggacctcc tgctggcgag agtaaccatc cgcggcccag
     8641 cccgcctggc cagaccgata aactggacaa agccccgtta tagtaacacc gcggcggatt
     8701 ggttgtcaaa cacgtgggct aggggtagtg tcctaatttg acttacgtcg tagctggata
     8761 gccacgtcga ttgccagccc gacgagcagt cccgcgccgc ccttgattgc cagttccata
     8821 accgcctgaa actcggtttg ctgtgaccac gtttgcataa acaggcagcc gacgactagt
     8881 ccgacgacaa gccaggggat gcgcataaaa aggccccgcc agtttcccag aggggcattc
     8941 tactacgccc ttaggccgca accttatctg ctggtcatcg aggccacgtc agtcgttgac
     9001 gacgacgaca agtcgagcaa gtaaacccca gccgcctacg ggcggctttt tcatgcgcgg
     9061 tccgcggtcg gtgattt
//
LOCUS       DASRJB010000886         8409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2785228, whole genome shotgun sequence.
ACCESSION   DASRJB010000886 DASRJB010000000
VERSION     DASRJB010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785228"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFB80_23345"
     CDS             complement(<1..199)
                     /locus_tag="VFB80_23345"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HZN36792.1"
                     /translation="MSDREFNNYLTLLAGLLRLGEKQRQAIAEELRSHLEDRLEELLA
                     SGMTREEAIRRALAEFGDAAGL"
     gene            complement(209..1021)
                     /locus_tag="VFB80_23350"
     CDS             complement(209..1021)
                     /locus_tag="VFB80_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36793.1"
                     /translation="MKMLVIGAAVAALIWGEAPTAAGQETKPESAKPRTSEPSRAADV
                     LSRQLDLEFKETPLKDVASSLSDRTGTTFFVRARALNDAGINLDAPVTGSFRHMKLGA
                     VLDLLLSELDLTYVDADEAVLITTPDDAESRLVARVYDCRDLMAGPLAVGPIAPPNAI
                     GAAPGAPRTDATPQALSLQQSHTPPAIGCGSIQGAWKVEGPAGQLIHVLTNGVDVQSW
                     SEVGGPGTISEYRGLFVVTQTARTHDKVERLLNQLREASGLADAAGKAKVVR"
     gene            complement(1089..1439)
                     /locus_tag="VFB80_23355"
     CDS             complement(1089..1439)
                     /locus_tag="VFB80_23355"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN36794.1"
                     /translation="MSDKFQRDLVRGSLDLMVLSVLAGGKKYGYLIQKEVREASGSRV
                     ELAAGTLYPLLHKLEDDGLIRSSWDASTGRERKWYELTAAGRKRLAAQAQEWSDYAAC
                     VKQLLAVARVQAAS"
     gene            1555..2496
                     /locus_tag="VFB80_23360"
     CDS             1555..2496
                     /locus_tag="VFB80_23360"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZN36795.1"
                     /translation="MTVSSRSLEHPSPAFVISAGTQVVLKVSKALADGSYRKPGSVAL
                     IVESPASNDGAYALQFADGQTATAYFRELVLRRQEVEAELGQLAADLRPYIIYRCQVG
                     SKAFGLDEADADDDLRGIYLPPARLHWSLYALPEQLESQTDLHDEVYWEVGKFLLLAL
                     KANPNVLETLWTERVLAATPLAQELRAIRGAFLSQHLFKTYSGYVLSQFRRMEAALRA
                     GGGYKPKHAMHLVRLLHCGIHALRTGEILVDVSARRDELLRIKRGELPFDAVKQQALA
                     LEREFQSAYETTQLPEQPDFARVNEFLLKARRSAVDA"
     gene            2505..3263
                     /locus_tag="VFB80_23365"
     CDS             2505..3263
                     /locus_tag="VFB80_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZN36796.1"
                     /translation="MIDLTVWKNHVAGLELRPLFVTVSGAHLYGFESPDSDVDLRGCH
                     LLPLREVVGLKRPNETTEIAGVHAGVEVDLVSHDAAKYFQLLAKNNGYVLEQIFSPLV
                     VTGQEFLDRLRPIAARCITRFHYFHYRGFIGTQRKLLAKEEPKRAKSLLYAYRVLLTG
                     IHLLRSGQVEANLRRLNERFQLPYIDELIAAKTGGERIGIPALDWALHDGELTRLEQQ
                     LESAFDSSPLGEQRDLTALNELLIELRLGRGEHY"
     gene            complement(3257..4213)
                     /locus_tag="VFB80_23370"
     CDS             complement(3257..4213)
                     /locus_tag="VFB80_23370"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36797.1"
                     /translation="MFRRNPTRQHGGFTLVELLVVIAIIGVLVALLLPAVQSAREAAR
                     RMQCSNNLKQIGIAMHNFHDTYLVLPPGAVTGTTAAANQVRTRFGIKGTGDQGWGMFI
                     YPYIEQKNLYDTYRWDLDFRDPNNQKAREQYVSTLICPTSPIQRRRDVMTSGGFTNWQ
                     AAASDYGVNNGVDAANLNPLGLVEPGTVGAPNGVMRVNETQRFAEITDGLSNTMWICE
                     DAGRPTVYRARKQRFTGRRAGSSVLDRENEYILHGFDAAGTSNPGPCHMNCTNGDEMY
                     SFHPVGVMCMFGDGSIRFVSDTTPFRVVAGWISRGGGENAGQ"
     gene            complement(4261..4728)
                     /locus_tag="VFB80_23375"
     CDS             complement(4261..4728)
                     /locus_tag="VFB80_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36798.1"
                     /translation="MIQAMSASRRSGLALSAIATALSCLGVLGCSGKGGSQKACYPVK
                     GQVFVKSQPADGALVTLSPRDAKQEEWPSGYPHARVAADGSFEVGTYAEKDGAPAGEY
                     VILVRWPAKNPNPDAEEVDGPDRLREKYSDPGSSRLNAKVEAKPTELPAIRIP"
     gene            complement(4859..5866)
                     /locus_tag="VFB80_23380"
     CDS             complement(4859..5866)
                     /locus_tag="VFB80_23380"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZN36799.1"
                     /translation="MSSKTVTTLLPPTSAAIHQAAETLREGGLVAMPTETVYGLAAAA
                     ENDVAVQAMFAAKGRPADHPVIVHLAEARQLDEFAALVPESARQLAAAFWPGPLTLVV
                     RRNARISPLVTGGLPTVGLRVPAHPVAQALLRAFRGPLAAPSANRFGRVSPTTAQHVL
                     GDLAGRVDLILDGGPCEVGLESTIVDCSGDAGSVVNVLRPGAITAEQIAQVLHSEVAV
                     ASAGSPRVSGSLPSHYAPRARVEIVPEAELFARAAALAAQGEKVALLSRQLPPTSTNN
                     VILLMIPADERQWAKNLYSLLRQVDERGCDAAVAALPTGEGLSAAIADRLRRAAGRRD
                     V"
     gene            complement(6006..6335)
                     /locus_tag="VFB80_23385"
     CDS             complement(6006..6335)
                     /locus_tag="VFB80_23385"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN36800.1"
                     /translation="MLHDPTAHAEMIAITQAANHVGDWRLEGCTLYVTLEPCVMCCGA
                     ILQARLPTVVYGATDPKAGAASTLFHLLSDERLNHRCKVIPGILAAPCGEILTRFFQQ
                     QRRLGKK"
     gene            complement(6302..6466)
                     /locus_tag="VFB80_23390"
     CDS             complement(6302..6466)
                     /locus_tag="VFB80_23390"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HZN36801.1"
                     /translation="MTHDHYMRQALAEAEQALVENEVPVGAVIVHGERVIAAATTSGR
                     CCTTRPPTPR"
     gene            6606..7910
                     /locus_tag="VFB80_23395"
     CDS             6606..7910
                     /locus_tag="VFB80_23395"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36802.1"
                     /translation="MNRRDFLNSSATFVTAAALASAAKLGLAAAGEPLNVAVIGPGGM
                     GSSHLRLLSVRKDLRVAYVCDVDANRLAAAAKVVEQNGGSPKAVKDLRQVLDDKSVDA
                     VWIATPDHWHSPAAILAVEAGKHVYVEKPMSHNVREGRLLVEAARRNKKVVQVGTQSR
                     STPTVMRAMEIVKSGQIGDVLVAKAWNSQLRGSIGKKQPSTPPPHLDFGLWLGPAPER
                     PYQDNLLHGIWRWWYDFGAGDIGNDGVHDIDIARWGLGVTGAPSTVAALGSKFFFDDD
                     QQFPDTQYCVFEWPGDGRPGQKRQLIFEQRDWSPYVQEGYENGAAFYGTKGLLVMGHT
                     SGFQVFGPRNAPGEKGSGGVDLPAHHQNFLDCIKNGGRPAADAEEGHQTAVLVHLANI
                     ACRVQRTLHLDPAAEKIVGDDEAQKLIRRTYRDGHWAVPKGV"
     gene            complement(7978..>8409)
                     /locus_tag="VFB80_23400"
     CDS             complement(7978..>8409)
                     /locus_tag="VFB80_23400"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36803.1"
                     /translation="DTVDGGDGDDRLYGGDGNDTISGDAGNDVVAGNAGNDRLYGRAG
                     NDVMIGGLGADIINGDEGNDLLFDGAVSLAGGTDASLTKNDANDLAMLAILTTWQAGI
                     IAAGVAHPHDGDIDSLNGDSGDDTGSPGPEDFFLDLETLLP"
BASE COUNT         1466 a         2763 c         2686 g         1494 t
ORIGIN      
        1 ccaggccggc cgcgtcgccg aattccgcca gtgcccgccg gatcgcctct tcccgcgtca
       61 tgccgctggc cagcaattcc tccagccgat cctccaaatg actccgcagt tcctccgcaa
      121 tcgcctgccg ctgcttctcg cccagccgca atagcccagc caggagcgtc aggtagttgt
      181 tgaattcgcg gtcggacatg cggttctcct aacgcacgac cttcgctttg ccggcagcgt
      241 cggccaggcc acttgcttct cggagttgat ttagcaagcg ctcgaccttg tcgtgggtgc
      301 gtgcggtctg agtcaccaca aacagccctc tgtactcgct gatggttccc gggccgccga
      361 cctcgctcca cgattgaacg tccacgccgt tcgtcaagac gtggatgagt tgcccagccg
      421 gtccctccac cttccaagcg ccttgaatgc tgccacagcc gattgccggt ggggtgtgcg
      481 actgttgcag ggagagcgct tgcggcgttg catccgtccg aggcgctcct ggcgcggcgc
      541 cgattgcgtt aggcggcgca atcggtccga ctgccaacgg gccggccatc aaatcgcggc
      601 aatcgtacac gcgcgccacc agtcgcgact ctgcatcatc cggagtcgta atcaatacgg
      661 cctcgtcggc atcgacgtag gtcaaatcga gctcggatag cagcaagtcg aggacggcgc
      721 cgagcttcat atgtcgaaag gaaccagtca cgggtgcatc cagatttatc cccgcatcgt
      781 ttagcgctcg cgctcgcaca aaaaacgtgg tgcccgttcg gtcgctcaag ctggaagcca
      841 catccttaag cggcgtttcc ttgaactcca aatccaattg cctgctcaag acgtcagcgg
      901 cccgcgacgg ctcacttgtt cgcggcttcg ccgattcggg cttcgtctct tgtcccgcgg
      961 ccgttggggc ctcgccccat atcagagccg ccacggccgc gccgatcacc agcatcttca
     1021 ccgagggaag cggtatcctt cgcatgatcc acatctccaa gctgcccccg aagaattaaa
     1081 cctccatatc aactcgccgc ttgcaccctg gccaccgcca agagctgctt cacgcaggcc
     1141 gcgtagtcac tccactcctg ggcctgggcg gcgaggcgct tgcggccggc ggcggtcaac
     1201 tcgtaccact tgcgttcgcg acccgtgctg gcgtcccacg agctgcggat cagcccgtcg
     1261 tcctccagct tgtgcagcag cgggtagagc gtgcccgccg ccagctcgac ccgcgagccg
     1321 ctggcctcgc gaacctcttt ctggatcagg taaccatatt tcttgccgcc ggcgagcacc
     1381 gacaggacca tcaggtccag gctgccgcgg accaggtcgc gctgaaactt gtccgacatc
     1441 cgaagtctcc ctgtcttgcg ccaatatata gtcgatcgat atatcggctg tcaacctatc
     1501 gggaaaggtt ttccgccggg gacccatgac gtttatctcg gctatcctta ccccatgacc
     1561 gtttccagcc gctcgctcga acatccctcg ccggcgtttg tcatctcggc agggacgcag
     1621 gtggtgctga aagtcagcaa ggccctcgcc gacggcagct accgcaagcc gggctcggtc
     1681 gcgttgatcg tcgagagtcc cgcatccaac gacggcgcgt acgccctcca gttcgccgat
     1741 ggtcagacgg ccaccgccta cttccgcgag ctcgtgctcc gccgccagga agtcgaggcc
     1801 gaactgggtc agctggccgc cgacctgcgg ccgtacatca tctaccgctg ccaggtaggt
     1861 tcgaaagcct tcggcctgga tgaagcggat gccgacgacg acctccgcgg catctacctg
     1921 ccgcccgcca ggctgcactg gtcgctgtat gccctgccgg agcagctcga atcgcaaacc
     1981 gacttgcacg acgaagtcta ttgggaggtg ggcaaattcc tgctgctggc cctgaaggcg
     2041 aacccgaacg tgctggagac cctctggacc gaaagagtgc tggcggcgac gccgctggcc
     2101 caggagctgc gggcgatccg cggagcgttc ctctcgcagc acctgttcaa gacctactcg
     2161 ggctacgtgc tcagccagtt ccgccgcatg gaggccgccc tgcgggccgg aggcggctac
     2221 aagcccaagc acgcgatgca cctggtgcgg ctgcttcact gcggcattca cgccctgcgg
     2281 accggcgaga tcctggtcga tgtctcggcc cgccgcgacg aactgctgcg gatcaagcgc
     2341 ggcgagctgc cgttcgacgc agtcaagcag caggcgctgg cgctagagcg cgagtttcaa
     2401 tcggcctatg agacgaccca gttgcccgag caacccgact tcgcccgcgt caacgagttc
     2461 ctcctgaaag cccgccgcag cgcggtggat gcgtgagcgc cgcaatgatc gacctgaccg
     2521 tctggaaaaa ccacgtcgcc ggcctcgagc tgcggccgct gttcgtcacg gtcagcgggg
     2581 cgcacctcta tggcttcgaa tcgcccgaca gcgacgtcga tctccgcggc tgccacctgc
     2641 tgccgctgcg cgaggtggtg ggcctgaaac ggccgaacga aaccacggaa atcgcgggcg
     2701 tccacgcggg cgttgaggta gatctggtca gccacgacgc ggccaagtac tttcagctgc
     2761 tggccaagaa caacggctac gtgctggagc agatcttttc gccgctggtc gtgacgggtc
     2821 aggagttcct cgatcgcctg cggccgatcg ccgcccgctg catcacgcgg ttccattact
     2881 tccactaccg cggcttcatc ggcacgcagc ggaagctgct ggccaaggaa gagcccaagc
     2941 gggccaaatc actgctctac gcatatcgcg tgctgctaac gggcattcac ctgctgcgct
     3001 ctgggcaggt cgaggcgaac ctgcggcggc tgaacgagcg ctttcagctg ccctacatcg
     3061 acgagctgat cgccgccaaa acgggcggcg agcggatcgg cattcccgcg ctcgactggg
     3121 cgctgcacga cggcgagctg acccgcctgg agcagcagct cgaatcggcg ttcgacagct
     3181 cgccgctggg cgaacagcgc gacctgaccg ccctgaacga gctgctgatc gagttgcgcc
     3241 tgggccgcgg cgagcattat tgaccagcgt tctcgccgcc tccgcgtgag atccagcccg
     3301 cgaccactcg aaacggagtc gtgtccgaga cgaaccgtat cgaaccatcg ccgaacatgc
     3361 acatcactcc aacgggatga aacgagtaca tttcgtcgcc gttggtgcaa ttcatatggc
     3421 agggccccgg attagatgtc cccgctgcat cgaatccgtg caggatgtac tcgttttccc
     3481 gatcgagaac cgacgaaccc gcgcgccgtc cggtgaatcg ctgcttgcga gcgcggtata
     3541 cggtcggccg cccagcgtcc tcgcagatcc acatggtgtt ggaaagaccg tcggtaatct
     3601 cggcgaaccg ctgcgtttcg ttgacccgca tcacgccgtt gggagcgcca acggttcccg
     3661 gttcaaccag tcccagggga ttgaggttgg cggcgtccac tccattattc acgccgtagt
     3721 ccgaagcggc ggcctgccaa ttggtgaatc cgcctgatgt catcacgtcc cgccggcgtt
     3781 gaatcggcga ggtcgggcaa atcagcgtgc tgacatactg ctcacgagcc ttttgattat
     3841 tcggatcgcg aaaatccaag tcccagcgat aggtgtcgta cagattcttc tgctcgatgt
     3901 aagggtaaat aaacatgccc caaccttggt cgccggttcc cttaattcca aaacgtgtgc
     3961 ggacctgatt cgccgcggca gtggtgcctg tgaccgcccc gggaggcaac acgagatacg
     4021 tgtcgtgaaa attgtgcatc gcaatgccga tctgctttag attgttgctg cattgcatgc
     4081 gccgcgcggc ctcgcgagcg gattggacgg caggaagaag cagtgctacg agcacgccga
     4141 tgatggctat gaccaccagg agttcgacca gtgtgaagcc gccgtgctgg cgcgtcggat
     4201 tgcgtcggaa cataagtcaa gaccccaagg aattcagttc gctgctgtgg acagcccggt
     4261 tcaggggatt cgaatcgcgg gcaactcggt tggcttagct tccaccttcg cgttaagcct
     4321 tgatgagccg ggatcggaat atttttcgcg aagtcggtcg ggaccatcga cttcttcggc
     4381 atccggattt gggttcttgg ctggccacct caccaagatg acatactcgc ctgccggagc
     4441 gccatctttc tcggcatagg tcccaacctc gaacgagccg tcggccgcga ccctggcgtg
     4501 gggatagccg ctgggccact cctcctgttt ggcgtcgcgg gggcttaaag ttaccagcgc
     4561 gccatccgcc ggctgcgatt tgacgaacac ttgccccttc acgggatagc acgccttctg
     4621 cgagccgcct ttgccggagc agccaaggac gccgaggcac gacagcgcgg tggcgatcgc
     4681 cgaaagtgcc aacccagacc ggcggcttgc tgacattgcc tgaatcacga cgcggcgtcg
     4741 tcaacggaaa ggcgggagcg gcccttcgcg gccgcatcgc aggggaacac taaaatacca
     4801 agccagtggc gaatttggta cgacttgcgc gtggaatggg accggattta ctcagaattc
     4861 acacatctcg ccggccggcg gcgcggcgga ggcggtcggc gatggcggcg gaaagccctt
     4921 cgccagtcgg cagtgcggcg acggctgcgt cgcacccccg ttcatccacc tgtcgcagca
     4981 gggagtaaag attctttgcc cactggcgct cgtcggcggg aatcattagc aagatgacgt
     5041 tgttagtgct cgttggcggc agctggcggc taagcagggc gaccttttcg ccttgggctg
     5101 ccaatgccgc ggcacgggcg aagagttccg cctcgggaac gatctcgacg cgggcccgcg
     5161 gcgcataatg gctgggcaaa ctgccgctga cgcgtggcga cccggcggat gccacagcga
     5221 cctccgagtg cagcacctgg gcaatctgtt cggccgtgat cgcgccgggg cggagcacgt
     5281 tgacgacgct gccagcgtcg ccactgcagt ccacgatcgt cgattcgaga cccacttcgc
     5341 acggcccgcc gtcgaggatc aaatcgacgc ggccggccag gtcgccgagc acgtgctggg
     5401 cggtcgtcgg gctgacgcgt ccaaagcgat tggccgacgg cgcagccagc ggcccgcgaa
     5461 acgcgcgcaa tagcgcctgc gccaccggat gcgctggcac gcgcagtccc acggtcggca
     5521 ggcctccggt gacaagtggg ctgatccgcg cattgcggcg cacgaccagc gtcagcggcc
     5581 cgggccagaa cgcagccgcg agttggcggg ccgactcggg cacgagcgcg gcgaactcat
     5641 cgagctgtcg agcttccgcc aggtgcacga tcaccggatg gtccgctgga cgccccttgg
     5701 cggcgaacat ggcctgcacc gccacatcgt tctcggcagc ggcggccagg ccatacaccg
     5761 tttcggtagg catggcgacg aggccaccct cgcggagcgt ctccgccgcc tggtgaatgg
     5821 ccgcggaggt tgggggcagg agtgtggtca ccgtcttgct gctcatcctg atcttcctct
     5881 taatcctgct cgtaatcctc ttcctaatct taatcttcgt cttgctcctt ctcgctgccg
     5941 gacggaggag caagagtaag agtaggatta ggatggactg actgaccttc cccacgacgc
     6001 gtcgcctact ttttcccaag ccgccgctgc tgctggaaga agcgagtgag aatctctccg
     6061 cagggagcgg cgaggatgcc ggggatgact ttgcagcggt ggttcagccg ctcgtcgctg
     6121 agcaagtgga acagcgtgct cgcggcgccg gccttcgggt ccgtcgcgcc gtagacgacg
     6181 gtcggcagcc gggcttgcag gatcgcgccg cagcacatga cgcagggctc gagcgtgacg
     6241 tagagcgtgc agccttccag ccgccagtcg ccgacgtggt tggcggcctg ggtgatcgcg
     6301 atcatctcgg cgtgggcggt cgggtcgtgc aacatctccc gctggttgtg gcggcggcga
     6361 tcacccgctc gccatgcacg atcaccgcgc cgaccggcac ttcgttctca accagcgcct
     6421 gctcggcttc ggccagagcc tgccgcatgt aatggtcgtg ggtcacgggg agagtgctgg
     6481 tgctggtggc tagtgcttgg ggacggaaac gcatcataca ccgccagaca cagttgaatt
     6541 cctcatcaac gcgcggtcga atttgtttcc gtccctagcc cccagcacca gcccccagca
     6601 ctcctatgaa ccgccgagat tttctcaact cgtccgcgac cttcgtcacc gccgcggcat
     6661 tggcttccgc ggcgaagctc ggcctggcgg cggcgggtga gccgctcaac gtggcggtga
     6721 tcggcccggg cggaatgggt tcgagccacc tgcggctgct gtcggtccgc aaggatttgc
     6781 gggtggccta cgtctgcgac gtggacgcca accggctggc ggccgccgcc aaggtcgtgg
     6841 aacaaaacgg cggctcgccc aaggcggtga aagacctgcg gcaggtgctc gacgacaaat
     6901 cggtcgatgc ggtctggatc gcgacgcccg accactggca ttcgccggcg gcaatcctgg
     6961 ccgtggaagc cggcaagcac gtgtacgtcg agaagccaat gtcgcacaac gtccgcgagg
     7021 gccgcctgct ggtcgaggcc gcgcggcgga acaagaaggt ggtgcaggtc ggcacgcaaa
     7081 gccgcagcac gccgacggtg atgcgggcga tggagatcgt caagagcgga cagatcggcg
     7141 acgtgctggt ggccaaggcg tggaacagcc agctccgcgg cagcatcggc aagaagcagc
     7201 ccagcacgcc tccgccgcac ctggattttg gcctctggct cggccccgcg cccgagcggc
     7261 cctatcagga caacttgctg cacggcatct ggcggtggtg gtacgacttc ggcgccggcg
     7321 acatcggcaa tgacggcgta cacgacatcg atatcgcccg ctggggcctg ggcgtcacgg
     7381 gagcgccctc gaccgtcgcg gccttgggca gcaagttctt cttcgacgac gaccagcagt
     7441 tccccgacac gcagtattgc gtcttcgaat ggccgggcga cggccggccc ggccagaagc
     7501 ggcagctcat cttcgagcag cgcgactggt ccccctacgt gcaggaaggc tacgagaacg
     7561 gcgcggcctt ttatggcacg aagggcttgc tggtcatggg gcacaccagc ggcttccagg
     7621 tcttcggccc ccggaacgcg cccggcgaga aaggcagcgg cggcgtggac ctgccggccc
     7681 accaccagaa tttcctggac tgcatcaaaa acggcggccg gcccgccgcc gatgccgaag
     7741 agggtcacca aactgccgtg ctcgtccacc tggcgaacat cgcctgccgc gtgcagcgaa
     7801 cgctgcacct ggatcccgca gccgagaaga tcgtcggcga cgacgaagcc cagaagctga
     7861 tccgccgcac ttatcgcgac ggacactggg cggtgccgaa gggagtgtga gcgagtccgg
     7921 cacgcggcag gtggctcgcg tgtcgccagt cccgatcgga gggaagaggc cggcatccta
     7981 cggcagcagt gtttccaggt cgaggaagaa gtcttcgggg ccgggggagc cggtgtcgtc
     8041 accggagtcg ccgttgaggg agtcgatgtc gccgtcgtgg ggatgggcga cgcccgcggc
     8101 gatgatgccg gcttgccagg tcgtcagaat cgccagcatg gcgaggtcat tggcgtcgtt
     8161 cttcgtgagg ctggcgtcgg tgccgccggc gagcgagacg gcgccgtcga agagcaggtc
     8221 gttgccttcg tcgccgttga tgatgtcggc gccgaggccg ccgatcatca cgtcgttgcc
     8281 ggcgcggccg tagaggcggt cgttgccggc gttgcccgcg accacatcgt tgccggcgtc
     8341 gccgctgatg gtgtcgttgc cgtcgccgcc gtacagccgg tcgtcgccgt cgccgccgtc
     8401 gacggtgtc
//
LOCUS       DASRJB010000887         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2231959, whole genome shotgun sequence.
ACCESSION   DASRJB010000887 DASRJB010000000
VERSION     DASRJB010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231959"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFB80_23405"
     CDS             <1..1550
                     /locus_tag="VFB80_23405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZN36804.1"
                     /translation="LLTTDQPGLDADLAIEEAISSAAGLVLRSADNAIVRGPLTAATT
                     IDIAIDTANADPGEGNTLVLLAAIIAPGGTTAAGNSDADRFEIVPQTGSALLIVGGLP
                     IAPQSNPQSQNTTSGDVFGDKLVLDMTTAGDGQATLGPVIVDSVGGRATAENTRLLTY
                     RGIEDLDLFDGSLLTTTQQGDIYVRGTGADERIYITHDGGTNPQVRVSVAGRTFPGSQ
                     GYFGPYVRGRSVIVYARGGHDLVELSSTPLRGEFHGEGGDDYLSAASFADLLIGGPGS
                     DTLIGGSKGGHDELWGDDFDPIPQDDQSQPIANPTAAQLQQNREHWALLVSATDGPDK
                     LSTTSGNDRLYGQGGDDQLNPGAGDDYASGGSGNDKISGGDGNDRLYGDGGNDELAGD
                     AGSDLLSGGDGNDVLAGHRGNDVLIGGDGADNLHGIEGNDLLFAGRLALAGGDEDSTV
                     AGDARDAALLALLLEWSTLGQATGATSAGDDGDLDWLYGYAGSDRFVGDGSSRNYDFE
                     AGVDAVG"
     gene            complement(1616..>2829)
                     /locus_tag="VFB80_23410"
     CDS             complement(1616..>2829)
                     /locus_tag="VFB80_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36805.1"
                     /translation="LASIAVALACGIPLSFVAWEMFRDKSWATRGEWGLELAIHSVFP
                     FALTLFVSLVTWLCSVGWGHAAGWKPRLARAGVFLLALTAGLPGLWIYWQMLWYAPYP
                     KVNVGPENQYDRLITIAKRTNAFFNGGSMPPAVAAELDEAVLLVRQHNQVPQHVIESR
                     WNPETGLTGHDLMAITGRFREAAENAVRAREFDRLADCGVAIIRFDAMVGQGEQGHFA
                     YQGYELLSLHRREFSPAKARDVIGLLRQTIAERYDVEFLIACQRAQSERMTGWHTKLR
                     SALERLTGKSRKDGWSSQAAQHYRETRKGWDVMNLLLQTDMAIGVFRSDHGRLPATLS
                     ELVPAYIPEMPVDPHSGRALVYRPRTGSFELYSVGPNGTDDGGRVPPRTGPPGDTRYD
                     IPLEPPRAGKQ"
BASE COUNT          517 a          904 c          962 g          446 t
ORIGIN      
        1 cgctgctgac gacggaccag cccgggctcg atgccgacct ggcaatcgag gaggctattt
       61 catcggcggc tggcctggtg ctgcggtcgg cggacaacgc gatcgtgcgc gggcctctta
      121 cggcggccac gaccatcgac atcgccatcg acacggccaa tgccgatccc ggcgaaggaa
      181 atacgctcgt tctgctggcg gcaatcatcg cacccggcgg cacgacggcg gctggcaaca
      241 gcgatgccga ccggttcgag atcgtgccgc agaccgggtc ggcgctattg atcgtcggcg
      301 gcttgccgat cgcgccccag tccaatccgc agtcgcaaaa caccaccagc ggcgacgtct
      361 tcggcgacaa gctggtcctg gacatgacga ctgccggcga tggccaggcg acccttgggc
      421 cggtgatcgt cgattcggtc ggcgggcgcg caaccgcgga gaacacgaga cttcttacct
      481 accgcggcat cgaggacctc gacctgtttg acggcagcct gttgaccact acccagcagg
      541 gggatattta cgtccgcggc accggcgcgg acgagcggat ctacatcacg cacgacggcg
      601 gcaccaatcc acaggtgcgg gtcagcgtgg cgggacgcac gttccccggc agccaggggt
      661 attttgggcc gtacgtccgc ggccggtcgg tcatcgtgta tgcccgcggc gggcacgatt
      721 tggtggagct gagctcgact cccttgcggg gagagttcca cggcgaaggc ggggacgact
      781 atttgtcggc cgcgtctttc gccgatctgc tcatcggcgg accggggagc gacacgctga
      841 ttggcggctc gaagggcggc cacgacgagc tgtgggggga cgacttcgat ccgatccccc
      901 aggacgacca atcgcagccg attgccaatc cgaccgcggc gcagctccag caaaaccgcg
      961 agcactgggc gctgctcgtc agtgccaccg acggcccgga caagctttcg accaccagtg
     1021 gcaatgaccg gctgtacggc cagggtggag acgaccagct caatccggga gcgggggacg
     1081 actacgccag cggcggcagc ggcaacgaca agatcagcgg cggcgacggc aacgaccggc
     1141 tgtatggcga tggcggaaac gacgaactgg ccggcgacgc agggagcgac ctgctgtccg
     1201 ggggcgacgg caacgacgtg ctcgccggtc accggggcaa cgacgtgctg atcggcggcg
     1261 acggcgccga caacctccac ggcatcgaag gaaacgatct gctgtttgcc ggccgcctgg
     1321 cgctggccgg cggcgacgag gacagcaccg tcgccggcga cgcccgcgat gcggcactgc
     1381 tggcgttgct gctagagtgg tccacgctcg gccaggcgac cggcgctacc agcgcgggag
     1441 acgacggcga cctggactgg ctgtatggtt acgccggcag cgaccggttc gtcggcgacg
     1501 gcagcagccg gaactatgat tttgaggcgg gggtcgatgc ggtggggtga tgggagggtg
     1561 gagtgcggcg tgtgcaaagc gcagagtgca gagtgaggag cgatggcgag ggcagtcact
     1621 gcttgcctgc gcgggggggt tcaagcggga tgtcataacg tgtatcccct ggtggacccg
     1681 tgcggggcgg gactcgaccg ccgtcgtccg ttccgtttgg tccgacgctg tacagttcga
     1741 acgatccagt gcgcgggcga tagactagcg cccggcccga atgtgggtcc acgggcattt
     1801 ctggaatata tgcgggcacg agctccgaga gggtcgccgg cagccggccg tggtcgcttc
     1861 ggaacacgcc gatcgccatg tcggtttgca gcagcaggtt cattacgtcc caccccttgc
     1921 gtgtctcgcg ataatgttgc gccgcttgcg aagaccagcc gtccttacgc gacttgccgg
     1981 tcagtcgctc caaggccgac cgcaatttgg tgtgccagcc cgtcattcgt tcggactggg
     2041 cccgctggca ggcgatgagg aactcgacgt cgtaccgctc ggcaatcgtc tggcgcagca
     2101 gcccgatgac atcgcgcgct ttggccgggg aaaactcccg gcggtggagg gagagcaatt
     2161 cgtagccctg ataagcgaag tggccctgtt ccccctgccc caccatcgca tcgaagcgga
     2221 tgatggccac gccacagtcg gccaagcggt caaactctct tgcccggacc gcattttcgg
     2281 ctgcctcgcg gaatcgcccc gtgatggcca tcaggtcgtg gccggtgagc ccagtctccg
     2341 gattccagcg cgactcgatg acgtgctgcg gcacctggtt gtgctggcgc accagtaata
     2401 cggcttcgtc caactcggcg gcaaccgccg gcggcattga gcctccgttg aaaaaggcat
     2461 tggtgcgctt ggcgatggtg atcagccggt catactgatt ctccgggcca acgtttacct
     2521 tgggatatgg cgcgtaccac agcatctgcc agtagatcca caatcccggc agtccggccg
     2581 tcagcgcgag caaaaacacg ccggcgcgcg ccaagcgggg tttccagcca gccgcgtgac
     2641 cccagcccac agaacaaagc cacgtcacca gcgagacaaa aagcgtcagg gcaaacggaa
     2701 agaccgaatg gatcgccagt tccaggcccc attccccgcg ggtcgcccaa ctcttatcgc
     2761 ggaacatttc ccacgccaca aaactgagcg gaatgccgca ggccagcgcg acggcgatcg
     2821 acgcgagga
//
LOCUS       DASRJB010000888         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1116230, whole genome shotgun sequence.
ACCESSION   DASRJB010000888 DASRJB010000000
VERSION     DASRJB010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116230"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFB80_23415"
     CDS             complement(<1..631)
                     /locus_tag="VFB80_23415"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZN36806.1"
                     /translation="MLQKFNEKNRDLIVNINGALVHRDQAGVSPFDSVVQGGDAVWEG
                     LRLYQGRIFRLHEHLDRLRSSALALAFTQIPTHEQIIGQIKRTLAANQMTDGVHIRLT
                     LTRGVKLTSGMDPRLNQAGPTLIVLAEHKPPVYDKGGLTLVTSSVRRFPPDCLDPKIH
                     HNNLIQSILAKIEANVAGADDALMLDTRGFVAETNATHLFLVSRGELATP"
     gene            complement(645..1382)
                     /locus_tag="VFB80_23420"
     CDS             complement(645..1382)
                     /locus_tag="VFB80_23420"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36807.1"
                     /translation="MPQSTQLRLAMWSGPRNISTAMMRSWGNREDTVVVDEPLYAHFL
                     RVTRKAHPGIDEVIATGETDWQQVVAQLTGEIPGGQPIFYQKHMTHHLLPDIGREWLS
                     QLTNCFLIRHPREVLASYTKVIESPQLEDTGFSQQAEIFEYVWKETGRVPPVIDAADV
                     LRDPRRLLGLLCDELGVAFQDAMLSWPAGPRTTDGVWAKYWYAEVEKSTGFRPYRPKQ
                     IELPDWLEGIYAECLEDYELLYPHRLR"
     gene            complement(1407..2591)
                     /locus_tag="VFB80_23425"
     CDS             complement(1407..2591)
                     /locus_tag="VFB80_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36808.1"
                     /translation="MQADFNPYHVWFGIPPEDQPADHYRLLGVRRFESNADVIANGME
                     QRIQFLRSRQIGKHQALSQRLANEVSAAAGCLLDPQQKAAYDLQLRHQHAAAATGQPV
                     IPIAPLTPIVVGPARESPLVEGPRPSAGLSSGTAATIVIAGLLLSMSILALGLWAAGV
                     FDSPEPTAASPAAALPPAALPPATPVKLVPPPASTPPPPIPPQSATKITAPAPGTIDL
                     LRRGAEQMVVKRGSVARRKGELCLDGAATALAIPATFPEQYAVEAEVTRESGNNSLCF
                     GFPVHGRPLTAIVDGFHSTRSGLACIDGHELYSPACPFVREGAVLTNGQKATVRIEVW
                     RKYVELAVNGRWIVRWEYDPRARVRSAQGMAAPQLDQLHLFAWDSSYRISRLELVPLS
                     GP"
     gene            complement(2594..4354)
                     /locus_tag="VFB80_23430"
     CDS             complement(2594..4354)
                     /locus_tag="VFB80_23430"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZN36809.1"
                     /translation="MSRLCLILGIFALGLVTRNLSAQDEAVTRGFRDLLASEWEYTLR
                     EAPTFASHLGDKRYNDRWPDVGLAAIERRHQHQKDVLQKLAAIDPEKLPPADRLNYLL
                     FRKEIAEGVEQYPFHWHLVALNQREGIQDESQLADALSFTKVKDYEDWTSRLRSFPAY
                     MDQTIELLRAGINEKMVQPKVTMRRLPEQIKKQLVDDPQQSLYFKPLKRFPKDISAGD
                     QERLKKAAAAAIQENVIPAYRKFAKFFDEEYFPACLDEVGAWQLPRGQELYALRARTF
                     TTTSLTPDEIHAIGLKEVARIRGEMEAIVKQVGFPGSFQEFLADLRTNKKFYYDDPDE
                     LMAGYLALCKRVDPQLTKLFKTLPRIPYGLEPIPAQIAPDTTTAYYRQPSADGLRPGT
                     YFVNLYRPETRPKYEMEALSLHESVPGHHLQLALQAELGELPPFRRYGGFTAFVEGWG
                     LYAESLGDQLGCYEDPYSKFGQLTYEMWRAVRLVVDTGMHSKQWPRQRAIEFFKENTA
                     KTELDIVNEVDRYISWPGQALAYKIGELKIKELRDRAKAQLGEKFDIREFHDVVLRQG
                     AVPLDVLEQIVDDWLAEQGK"
     gene            4536..>5044
                     /locus_tag="VFB80_23435"
     CDS             4536..>5044
                     /locus_tag="VFB80_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36810.1"
                     /translation="MSAAAAERPNILLIVSDDQGWPDLGCIGTKPIQTPHLDRLAKEG
                     VRLTNYYVTWPACTPSRGSILTGRHPLKSGLYDMVRNDLVNYGHRYTEQEYSTSPEMT
                     IGLDPREKTLGDVLRAAGYRCGMVGKWDMGQARRYLPLQRGFDFFYGIGNNGIDYYTH
                     ERYGIHSMFA"
BASE COUNT          878 a         1582 c         1640 g          944 t
ORIGIN      
        1 ggggcgtggc cagctccccg cggctgacga ggaacaggtg tgtcgcgttg gtctcggcga
       61 cgaagccgcg ggtgtcgagc atcagggcgt cgtcggcgcc ggcgacgttc gcttcgatct
      121 tggccaggat cgactggatc agattgttgt ggtggatctt gggatcgagg cagtccggcg
      181 gaaagcggcg gacgctgctg gtgacgagtg tcagcccccc tttgtcatag acgggcggct
      241 tatgctccgc caggacgatc agcgtcggcc cggcctggtt cagccgcggg tccatgccgc
      301 tggtcagctt cacgccgcgg gtcagcgtca ggcggatgtg aacgccgtcg gtcatctgat
      361 tggcggccag cgtgcgcttg atctggccga tgatttgctc gtgcgtcgga atttgggtga
      421 aggcgagggc cagagccgag ctgcggagcc ggtcgagatg ctcgtgcagg cgaaagatgc
      481 ggccttgata gagccgcagc ccttcccaga cggcgtcgcc gccttgcacg accgagtcga
      541 atgggctgac gcccgcctgg tcgcgatgaa ccagcgcacc gttgatgttg acgatcaggt
      601 cgcggttttt ctcgttgaat ttctgaagca tggcccccta ccacctaccg caggcgatgc
      661 ggatacagca gttcgtaatc ctccaagcac tccgcataga ttccttcgag ccaatcgggc
      721 agttcgatct gtttgggccg atacggccgg aagccggtcg acttctcgac ctcggcatac
      781 cagtattttg cccacacgcc gtccgtcgtc cgcgggcccg caggccagga gagcatcgcg
      841 tcctgaaagg caacgcccaa ctcgtcgcag agcaagccga gcagccgccg cggatcacgg
      901 agcacgtcgg ccgcgtcgat cacgggcggc acgcggcccg tttccttcca gacgtactca
      961 aagatctcgg cctgctgcga aaagccggtg tcctccagct ggggcgactc aatgaccttc
     1021 gtgtacgagg ccaggacttc ccgaggatgc cggatcagaa agcaattcgt gagctgcgac
     1081 agccactcgc ggccgatgtc cggcagcagg tggtgcgtca tgtgcttttg atagaagatc
     1141 ggctggccgc cgggaatctc gcccgtcaat tgagccacga cctgctgcca gtccgtttcg
     1201 ccggtggcga taacttcatc gatacccgga tgagccttgc gcgtcacgcg caggaagtgg
     1261 gcatagagcg gctcatcgac gacgaccgta tcttcgcggt tgccccacga gcgcatcatc
     1321 gccgtcgaaa tgttccgcgg cccggaccac atggcgaggc gaagttgggt ggactgcggc
     1381 atgcggcggt cctcggacaa ggacaatcac ggcccgctca gcggcaccag ttcgagccga
     1441 ctgatacggt acgacgagtc ccaggcgaac aggtgcagct gatcaagctg gggcgcagcc
     1501 attccctggg cgctcctcac ccgggcccgc ggatcgtact cccagcggac gatccagcgg
     1561 ccgttgacgg ctagctcgac gtacttgcgc cagacctcga tgcgaaccgt cgccttctgc
     1621 ccgttggtga gcacggcacc ctccctgacg aacgggcagg cgggcgaata cagctcgtgg
     1681 ccgtcgatgc aagccaggcc gctgcgcgtg gaatggaagc catccacgat cgccgtgagc
     1741 ggccggccgt ggacgggaaa accaaagcac agcgagttgt tccccgactc gcgcgtcacc
     1801 tcggcttcaa ccgcgtactg ctcgggaaac gtggcgggaa tcgccagcgc cgtcgcggcc
     1861 ccatccaggc acagctcgcc tttccgccgc gctaccgagc cgcgcttcac caccatttgt
     1921 tccgcgcccc gccgcagcag gtcgatcgtg cctggtgcgg gggcggtaat cttagtcgcc
     1981 gattgcggtg ggatcggcgg cggaggcgtg ctggccggag gtggaacgag cttgacggga
     2041 gttgccggtg ggagagctgc cggcgggaga gcagccgctg gcgaagctgc cgtgggctcc
     2101 ggcgaatcga acacgccggc tgcccacaag ccgagtgcca ggattgacat cgacagcagc
     2161 agcccggcaa tcacgattgt tgctgctgtt ccactcgata gcccagccga tggacgcggt
     2221 ccctccacca gtggagattc acgggccggg ccaaccacga tcggcgtcag cggggcaatt
     2281 gggatgacgg gctggcccgt ggccgcggcg gcatgctggt gtcgcagctg caggtcgtag
     2341 gccgcctttt gctgcgggtc gagcaggcag cccgcggctg cggagacttc gttcgccagc
     2401 cgctgcgaga gcgcctggtg cttgccaatc tgccgcgagc gcaggaactg gatccgctgc
     2461 tccatgccgt tggcgatgac gtcggcgtta ctctcgaacc ggcgcacccc cagcaggcgg
     2521 tagtggtccg ccggctgatc ttccggcggg atgccgaacc agacgtgata gggattgaag
     2581 tcggcctgca tgtctacttg ccctgctcgg ccagccaatc atcaacgatc tgctccagca
     2641 catccagcgg caccgcacct tgccgcagga cgacgtcgtg gaattcgcgg atgtcgaact
     2701 tttcgccgag ctgcgccttg gcccggtcgc gcagctcctt gatcttcaat tcgccaatct
     2761 tgtaagccag cgcctgcccg ggccaggaga tgtagcgatc gacctcgttg acgatgtcca
     2821 gctcggtctt ggccgtgttc tctttgaaga actcgatcgc tcgctggcgc ggccactgct
     2881 tgctgtgcat gcccgtatcg acgaccagcc gcacggcccg ccacatctca taggtcagct
     2941 ggccgaactt cgagtacggg tcttcgtagc agccgagctg gtcgcccagg ctctcggcgt
     3001 agaggcccca gccttcgacg aacgcggtga agccaccgta gcggcggaac gggggcagct
     3061 cgcccagctc ggcctgaagt gcgagctgca agtggtgacc cggaacggat tcgtgcagcg
     3121 agagggcctc catttcgtac ttcggccgcg tctcgggccg gtagaggtta acgaagtacg
     3181 tgcccggccg caggccatcg gccgagggct gtcggtaata ggcggttgtc gtgtcgggcg
     3241 cgatctgcgc cggaatgggt tccaggccat agggaatccg cggcaacgtc ttgaacagtt
     3301 tcgtgagctg cggatcgact cgcttgcaga gcgccaagta gccagccatc agctcatccg
     3361 gatcgtcgta gtagaacttc ttgttcgtcc gcaggtcggc caggaactcc tgaaacgagc
     3421 ctggaaagcc gacttgcttg acgatcgctt ccatctcgcc gcggatgcga gcaacttcct
     3481 taagtccgat ggcgtgaatc tcgtcgggcg tgaggctggt cgtcgtgaac gtgcgggccc
     3541 gcagggcata cagctcctgc ccccgcggca attgccacgc gccgacctcg tcaagacacg
     3601 ccgggaagta ttcttcgtcg aagaatttgg cgaacttgcg ataggcggga atcacgttct
     3661 cttggatcgc cgccgctgct gccttcttca gccgctcttg atcgccggcc gaaatgtcct
     3721 tggggaacct cttcagcggc ttgaagtaca gactctgctg cggatcatcc acgagctgct
     3781 tcttgatctg ctccggcagc cgccgcatgg tcactttcgg ctgcaccatc ttctcgttga
     3841 tcccagcccg cagcagctcg atggtctggt ccatgtaggc cggaaacgac cgcaggcggc
     3901 tggtccagtc ttcgtagtcc ttgaccttcg tgaacgacaa ggcatcggcg agctggcttt
     3961 cgtcctgaat cccctcgcgc tggttgaggg ccaccagatg ccagtggaac ggatactgct
     4021 cgacgccctc ggcgatctct tttctgaaga gcaggtagtt caggcggtcg gccgggggca
     4081 atttctccgg gtcgatcgcc gccagctttt gcagcacatc tttctggtgc tggtgccgcc
     4141 gctcgatggc cgcgaggcca acgtcgggcc agcggtcgtt gtagcgcttg tcgcccaggt
     4201 gcgaggcaaa cgtcggcgct tcgcggagcg tgtattccca ctcgctggcc agcaggtcgc
     4261 gaaagccgcg ggtgacggct tcgtcctgag cggacaggtt gcgggtgacc aggcccaagg
     4321 cgaaaattcc caggatcaag cagagtcgag acatcggtcg gccttctgga cgagacgctc
     4381 gctatcctat cttcgcgacg tatgctgcga cagaatgggg cggcgttaaa cactaaggca
     4441 cgaagatcgc gaagaaggcg aggaaggcaa tgcgaggaat gattctggcg ctattgtctt
     4501 tcctgcttgt ttcgtgtcct ttgtgccttc gtgttttgtc tgcagccgcc gccgagaggc
     4561 cgaacatcct gctgatcgtc agcgacgacc agggctggcc ggatctgggc tgcatcggca
     4621 cgaagcccat ccagacgccg cacctcgacc gcctggcgaa ggagggcgtg cggctgacga
     4681 actattacgt gacgtggccg gcctgcacgc catcgcgggg aagcatcctc accgggcggc
     4741 atccgctcaa gagcggcctg tacgacatgg ttcgcaacga cctggtgaac tacggccacc
     4801 gctatacgga gcaggaatat tcgacgtcgc ccgaaatgac catcggcctg gacccgcgcg
     4861 aaaaaacgct gggcgacgtg ctgcgagcgg ccggctaccg gtgcggtatg gtcggcaagt
     4921 gggacatggg ccaggcccgc cgctatctcc cactgcagcg cggcttcgac ttcttctacg
     4981 gtatcggcaa caacgggatc gactactaca cgcacgagcg gtatggcatc cattcgatgt
     5041 tcgc
//
LOCUS       DASRJB010000889         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3368353, whole genome shotgun sequence.
ACCESSION   DASRJB010000889 DASRJB010000000
VERSION     DASRJB010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368353"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VFB80_23440"
     CDS             complement(<1..1661)
                     /locus_tag="VFB80_23440"
                     /inference="COORDINATES: protein motif:HMM:NF026199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HZN36811.1"
                     /translation="MERRFLIFLVLMALLWGGFFALQAVLGPKRPPPVAKQPADKAGK
                     AAQPDQAKQPPEAAVAEVKPPAPAAPVAGVPLETGFLGHLDPAKGYSMLVKWVNHGAA
                     IERVELNSPRYKSVEDFSGYLGHLDLTNPNGGGAEVQAVGPGTPASLAKPHAGNMPPG
                     LKVGDVIKKIDGRDVADRHAVEDYLREKTHPGQSIVLDVQRPGTPQAISFTAQLARRP
                     LEVVRPESHTYKTDTGGLRVMPQDPLSLLVTLDNISGRSIRAGDVEIAELPSLYQSNW
                     KVEKSGEDFVEFSFTLDESQLEKIHKTGAIKIIKRYTLAKNEKPEQPGYHLDLAVEIH
                     NLGQAEQTVGYRFDGPTGLPTEGWWYSNKLNPKMFKAAGARDVVWDQPGVGHQLLGCP
                     MIVSQSKSLLEQQKPALLPLLAGDEAAPLTYVGVDTQFFASAVQPKSDIKFRAAYALP
                     VQDVTVIPKNWLRTTNVSFRLSTNPTAIQPRDFLKHEYRVFFGPKDPKVLDQYALGRF
                     IEYGWSIFKYPALLLLYVLKMVEGATRNYGIAILLLTVMVRSCMIP"
     gene            complement(1716..1922)
                     /locus_tag="VFB80_23445"
     CDS             complement(1716..1922)
                     /locus_tag="VFB80_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36812.1"
                     /translation="MTPAPTGRDPGGTPAADFAPVGLARLQTPTTQGSRLRRSALGLT
                     KVGPLGLHQTAIPLHLQETPLAGE"
     gene            complement(2043..2792)
                     /locus_tag="VFB80_23450"
     CDS             complement(2043..2792)
                     /locus_tag="VFB80_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZN36813.1"
                     /translation="MKRAILSDIHANLEALEAVLADISSVGVDSIYCLGDIVGYGPNP
                     RECIDRVMGFNLSILGNHDQGALYDPEGFSSGAERAIFWTRDQLEMANGDPDLTTRRW
                     QFLSELPRTHRDGQFTFVHGSVRNPLSEYVFPEDVHNARKLERLFAMIEKYAFQGHTH
                     VPGIFTEDGCFFSPGEIGSRYPLPGNKLMINVGSVGQPRDGDPRACYVILTDNTIEFR
                     RVPYPVERTAQKIYAVPELDPFLGDRLREGR"
     gene            complement(2884..3636)
                     /locus_tag="VFB80_23455"
     CDS             complement(2884..3636)
                     /locus_tag="VFB80_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZN36814.1"
                     /translation="MKRALISDIHANLEALRVVLYDIGSQGIREIYCLGDIVGYGPNP
                     CECLDEIITKSKVTILGNHDQATLFDPDGFNPIALRAVYWTREQLEVGPGPTHVKNKR
                     WDFLGELHRTHTEGDYLFVHGSPREPTNEYVFPEDIYNQRKMDALFGRIQKYCFQGHT
                     HLPGIFTADYRFITPEDCNYQYKLDDQRVMINVGSVGQPRDGDPRACYVILEDGLVTF
                     RRLEYPAEETRRKIYAVPDLDNALGDRLISGR"
     gene            3830..4510
                     /gene="tsaB"
                     /locus_tag="VFB80_23460"
     CDS             3830..4510
                     /gene="tsaB"
                     /locus_tag="VFB80_23460"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZN36815.1"
                     /translation="MLILAFDTSGLAGSVALLEGPRLLDQRQLDPARRSAQTLAPAIA
                     ELLAGQGVQPRQVGLVATTIGPGSFTGLRVGVTTAKTWAYATGGAVIGLSTLAVIAQQ
                     VPAELRSAAREIHAVLDAQRKELYFGRFHPSGERLAGDCIVPADAWLAGLTAGTIVTG
                     SGLSRLADRLPAGVTPAPQASWEPQAATVGKLAWEDYQRGRRDDFWTLAPVYLRTSYA
                     EEKRGSGI"
BASE COUNT          782 a         1445 c         1479 g          854 t
ORIGIN      
        1 gggatcatgc aggagcggac catcaccgtc agcaggagga tcgcgatgcc gtagttgcgc
       61 gtcgctccct ccaccatctt cagcacgtag agcagcagca gggcagggta cttgaagatg
      121 ctccagccgt attcgatgaa ccggcccagc gcgtactggt ccagcacctt cgggtctttc
      181 gggccgaaga acacgcgata ttcgtgcttc agaaagtcac ggggctggat ggccgttggg
      241 ttggtcgaaa ggcggaacga gacgttcgtc gtccgcagcc agttcttcgg gatgaccgtc
      301 acgtcctgca ctggcagggc atacgcagcg cggaacttga tatccgattt gggctgcacc
      361 gcgctggcga agaactgcgt atcgacgccg acgtacgtca gcggcgcggc ttcatccccg
      421 gccaacagcg gcagcagcgc gggtttctgc tgctcgagta aggacttcga ttgactgacg
      481 atcatcgggc agccgagcaa ttgatgacca actcccggct gatcccaaac cacgtcgcga
      541 gcgcccgcgg ccttgaacat ctttggattg agcttgttcg agtaccacca gccctcggtc
      601 ggcaagcccg tcggcccgtc gaagcgatag ccgaccgtct gctcggcctg gcccaaattg
      661 tggatttcaa cggccagatc gaggtgatag cccggctgct ccggcttctc gttcttggcc
      721 agcgtgtagc gcttgatgat cttgatcgcg ccggtcttgt ggatcttctc gagctgcgat
      781 tcgtcgagcg tgaagctgaa ttcgacgaag tcctcacccg atttttcgac cttccagttc
      841 gattggtaga gcgacggcag ctcggcaatt tcgacgtctc ccgcgcgaat gctgcggccg
      901 ctgatattgt cgagcgtcac gagcagcgac agcgggtcct gcggcatcac gcggagcccg
      961 cccgtgtcgg tcttgtacgt gtgcgattcc ggccgcacga cttccagcgg ccgccgggcg
     1021 agttgggcag tgaacgatat cgcttgcggc gttccggggc gctgcacgtc aagcacaatg
     1081 ctctggcccg gatgcgtctt ttcgcgcagg tagtcttcga cggcgtgccg gtccgcgacg
     1141 tcgcggccgt cgatcttctt gatcacgtcg ccgaccttca gtcccggcgg catgttgccg
     1201 gcgtgcggct tggccagcga cgcgggcgtt cccggaccga cggcctgcac ctccgcgccg
     1261 ccgccgttgg ggttcgtcag gtccaagtgg cccaaatagc cgctgaaatc ttccaccgac
     1321 ttgtaccgcg ggctgttcag ctcgacccgc tcgatcgctg cgccgtggtt gacccacttg
     1381 acgagcatcg agtagccctt cgctggatca aggtggccca aaaacccggt ctccagcgga
     1441 acccccgcaa ccggggccgc gggcgcgggc ggcttgactt ctgcaacagc cgcttcgggg
     1501 ggctgcttgg cctggtcggg ctgcgcggcc ttcccggcct tgtccgccgg ctgcttggct
     1561 acgggcggcg gccgcttcgg cccaagtacc gcctgaagcg cgaagaaccc tccccagagc
     1621 agggccatca ggaccaggaa aatcagaaat ctgcgttcca caaagcacta cctggggctc
     1681 ttgagcccgc tggccggcaa agagggcgta attcgttatt ctcccgccag gggtgtttct
     1741 tgcaagtgca gcggaatagc cgtttggtgc aggcccaacg ggccgacctt cgtcagccca
     1801 agggcgctgc ggcgcagccg cgagccctgg gtggtgggtg tttgcagccg cgccagcccc
     1861 accggggcga aatcggcggc gggggtgccg cctggatcgc gccccgttgg ggctggggtc
     1921 attcgtaacg ccgtcaggac ccagggcgat gccctgggct gacgaaggtc ggcccgttgg
     1981 gccgcagccg cgtggacacg gtggttaact ggcaatacta ggccactgcg caccggttgg
     2041 ccctaccgcc cttcccgcag ccggtcgccg agaaacggat cgagttccgg cacggcgtag
     2101 atcttctggg cggtccgctc caccggatac ggcacgcggc ggaactcgat cgtgttgtcg
     2161 gtcagaatca cgtagcaggc ccgcgggtcg ccgtcccgcg gctggccgac gctgcccacg
     2221 ttgatcatta gcttgttgcc gggcagcgga tagcgcgagc cgatctcgcc cggactgaag
     2281 aagcagccgt cctcggtgaa gattcccggc acgtgagtgt gcccctgaaa cgcgtacttc
     2341 tcgatcatcg cgaacagccg ctctagcttg cgggcgttgt gcacatcctc gggaaagacg
     2401 tattcgctga gcgggttgcg gaccgagccg tgcacgaagg tgaattgccc gtcgcggtgg
     2461 gtccgcggca gctcgctgag gaattgccag cggcgggtgg tcagatccgg atcgccgttg
     2521 gccatctcca gctggtcgcg ggtccagaag atggcccgtt cggcgccact cgaaaagccc
     2581 tccggatcgt acagcgcccc ttgatcgtgg ttgccgagaa tcgacaggtt gaagcccatc
     2641 acgcggtcga tgcactcccg cggattgggg ccgtagccga cgatgtcgcc caggcaatag
     2701 atgctgtcca cgcccacgct ggaaatatcc gccaggacgg cctccagggc ctccaggttg
     2761 gcgtgaatgt cgctgaggat ggctctcttc acagaaagaa atctcaagaa ccaaagaacc
     2821 aaagaaccaa acggactcca agctcttggt tcttcggttc ttcggttctt ggagttttcg
     2881 ccgctaccgc cccgagatca gccgatcgcc cagggcgttg tccaggtcgg gcacggcgta
     2941 gattttacgc cgcgtttctt cggccggata ctccagccgc cgaaacgtca ccagcccgtc
     3001 ctccaagatc acgtagcagg cccgcggatc gccgtcccgc ggctggccga cgctgccgac
     3061 gttgatcatc acccgctggt catccagctt gtattgatag ttgcagtcct cgggggtgat
     3121 gaagcggtag tcggccgtaa agatgcccgg cagatgcgtg tgcccctgga agcagtactt
     3181 ctgaatccgc ccgaagaggg cgtccatctt ccgctggtta tagatgtctt ccgggaagac
     3241 gtattcgttc gtcggctccc gcggcgagcc gtgcacaaac aggtagtccc cctcggtgtg
     3301 cgtgcgatgc aactcgccga ggaagtccca ccgcttgttc ttgacgtgcg tcgggccggg
     3361 gccgacttcg agctgctcgc gggtccaata gacggcccgc agtgcgatcg ggttgaaccc
     3421 gtcggggtcg aacagggtcg cctggtcgtg gttgcccagg atcgtcacct tggacttggt
     3481 aatgatctcg tccaggcact cgcaggggtt gggcccgtag ccgacgatgt ctcccaggca
     3541 gtagatttcg cgaatcccct gcgagccgat gtcgtataaa acgacccgca gcgcttcgag
     3601 gttggcgtgg atgtcgctga tcagggcacg cttcacgcca ctacctcata caacggacgc
     3661 tcgataatcg ggtacgtcaa aatgccctga tccgagcttg caagcagcgg cccgcgacat
     3721 tccgaggaaa cagccaagtc taggactggc ctagggtttg gtcaaggcaa gcggcgcgtg
     3781 ccaacagaca cgagcattac atcccagcca ttcagcccag cgcgcgatcg tgctaatcct
     3841 cgctttcgac acgtccggtt tggccggctc cgtggccctg ctggaagggc cgcggctgct
     3901 cgaccagcgg cagctcgacc cggcgcggcg atcggctcag acgctggccc cagccatcgc
     3961 ggaactgctg gcggggcagg gggttcagcc ccggcaggtc ggcctggtgg cgaccacgat
     4021 cggacccggt tcgttcaccg ggctgcgggt gggagtcacc acggccaaga cctgggccta
     4081 tgcgaccggc ggcgcggtca tcgggctgag cacgctggcc gtaatcgccc agcaagtgcc
     4141 cgccgaattg cggtcagccg caagggagat tcacgccgtc ctggatgccc agcggaagga
     4201 gctgtatttc ggccggtttc atcccagcgg cgagcgactg gcgggcgact gcatcgtgcc
     4261 ggccgatgcc tggctggccg gccttaccgc cggaacgatc gtcaccggca gcggcctttc
     4321 gaggctcgcc gaccggctgc cggccggcgt aactccggct ccacaggcca gttgggaacc
     4381 gcaggccgcc acggtcggca agctggcttg ggaagattac cagcgcgggc ggcgggacga
     4441 tttctggact ctggctccgg tctacctgcg aacgagctac gcggaagaaa aacgcggcag
     4501 cgggatatag cggaatgtga acattggtat gctatttgca gtatagttcc cattggcgcg
//
LOCUS       DASRJB010000890         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1955519, whole genome shotgun sequence.
ACCESSION   DASRJB010000890 DASRJB010000000
VERSION     DASRJB010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955519"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFB80_23465"
     CDS             complement(<1..857)
                     /locus_tag="VFB80_23465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZN36816.1"
                     /translation="MARTNTDGQPPAANQIIHGDAQAVLRRWPAELVDCIVTSPPYWK
                     QRDYRGERGQVGQERAAAEYVARLAALFGECRRVLKSTGTLWLVIGDKYEGGSQLGLP
                     WRVALALIDEGWTLRADCIWHKPNAMPSSTRSRPTTDHEYIFFFTKSPDYFYDADAVR
                     EPHVTFTDKSRMRGGRGHFGRRGGTPEAGKNGGTSNLHDGRWDQAFHPLGRNKRTVWS
                     IPLSKFREAHFAVFPEPLVETCLQAGCPAGGLVLDPFVGSGTVPLVARRLGRGYLGID
                     CVAEYCEMAR"
     gene            complement(871..1914)
                     /locus_tag="VFB80_23470"
     CDS             complement(871..1914)
                     /locus_tag="VFB80_23470"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN36817.1"
                     /translation="MISTRSLIQLCRRVGTAVKSGIDARRVWEMEERHATGPLKAALG
                     GIRQQIAHGGTVAEGMQGADGYFPPMFVQMVAIGEQTGKLDEVLHRLAEHYEHLATMQ
                     RTFWIGIAWPLFELVFAVLVIGLLILVTGIIGAVSGGDAPDVLGWGLVGPSGALVWFT
                     FCAFIAGGITLFCMAITRGWMGPQPMLLAMRIPVLGKCLESLALSRLTWSLALALDSG
                     MDARRAVELSIRAAQNPYYESSLPRVTAGIRANRQFHESFADGEVFPTEFLQQLETAE
                     LAGATTETLLRLATEYEDRARTSLRILTGIATVLVMLLVFGVMIFAIFSLFYHAYLKP
                     MHEALEMAETGRI"
     gene            complement(2086..3639)
                     /locus_tag="VFB80_23475"
     CDS             complement(2086..3639)
                     /locus_tag="VFB80_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36818.1"
                     /translation="MISRALLSAIVCGILLDTANAEPPKVSPPPESFFQIVAEGDREV
                     ARGFYSKYIDVNGMPVVAHADVADQALVRVYEIVTRMLAGRPDVTAKLVENRMYLIVM
                     GKDQVYTDMPEYRKHPNPAYQNERVRGTGGRPTSFGEENVLSLPLDRYDDESIAVHEF
                     CHTIDGTLRSIDPSWSQRKNDVYRNARSKGLHQDVYTGSNAGEYWAEICQAYFDCQRV
                     NNWNHNPIGTREQLKVYDPEAYELVRTTFNLSPEQDWRYSWLQKLPNVGPPPAKFKID
                     PYYTRFTWAREFTVVGRGASDAALLKANDTIRKMFAYRHDVLKALIADGVKLVVLGKG
                     ERIADLPEYALLKEMKDFDPLTRALAYSPETKLIVVGEENVGGDPRLPNVGDNQVIRL
                     MARAAYEVCGNRPVDPNWDRRRGVQQYELRVRRLDERFGQRLAELHKAAGEAEKWKGT
                     SAVHSPVNYWAAGVLAYFDALGQDGAPNDAPHPIRTRELLKDYDADLFALVEETMAYR
                     GKVDWRFTP"
     gene            complement(3729..>4168)
                     /locus_tag="VFB80_23480"
     CDS             complement(3729..>4168)
                     /locus_tag="VFB80_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36819.1"
                     /translation="PMSDSRSIATRLMGLAVGLLLVSSPGCGQTRFPVSGKVLFSDGT
                     PLDAGTVMGEATLEGGKTVMVQANIGKDGSFSLGTDQPGDGVPPGKYKLLVAPRAMSE
                     QEERTLPPFIDKKWERFDSSGLELDVTAPKSDFNITITRPKAR"
BASE COUNT          695 a         1371 c         1372 g          730 t
ORIGIN      
        1 cgggccattt cgcagtactc cgccacgcag tcgatgccca ggtagccgcg gccgagccgg
       61 cgggccacca acggcacggt gccgctgccg acgaacggat cgagcaccag gccgcccgct
      121 gggcagccgg cctgcaggca ggtctcgacg agcggctcgg gaaagacggc gaaatgcgcc
      181 tcgcgaaact tggagagcgg gatcgaccag acggtccgct tgttgcggcc cagcggatga
      241 aaagcctggt cccagcggcc gtcgtggagg ttgctcgtgc cgccgttctt gccggcctcg
      301 ggcgtgccgc cgcggcggcc aaagtgcccc cggccgccgc gcatccggct cttgtcggtg
      361 aaggtcacgt gcggctcgcg gacggcgtcg gcgtcgtaga agtagtccgg cgatttggtg
      421 aagaagaaga tgtactcgtg gtcggtcgtc ggccgcgacc tggtcgagct gggcatggcg
      481 ttgggcttgt gccagatgca gtcggcccgc agggtccagc cttcgtcgat gagtgccagc
      541 gcgacccgcc acggcaggcc gagctgcgag ccaccctcgt acttatcgcc gatcacgagc
      601 cagagcgtgc cggtggattt gaggacgcgg cggcactcgc caaagagcgc cgccaggcga
      661 gcaacatatt ccgccgccgc ccgttcctgg ccgacctgac cgcgctcgcc gcggtaatcc
      721 cgctgcttcc aatacggcgg gctggtcacg atgcaatcga ccagctccgc cggccaccgc
      781 cgcaagacag cctgcgcatc gccatgaatg atctgattag ccgcaggcgg ctgcccatcc
      841 gtgttcgtcc gtgccatccg cggttccttc ctaaatccgc ccggtttccg ccatctccag
      901 cgcttcgtgc atcggcttga gatacgcatg atagaacagc gagaagatcg cgaaaatcat
      961 gacgccgaag acgagcagca tcaccagcac cgtggcgatg ccggtcagaa ttcgcagcga
     1021 agtgcgggcg cggtcttcgt actccgtcgc caggcgcagc agcgtctcgg tcgtcgcgcc
     1081 ggccaactcc gccgtctcga gctgctggag gaactcggtc ggaaagactt cgccgtcggc
     1141 gaagctctcg tggaactgcc ggttggcccg gatgccggcg gtcacccgcg gcagcgacga
     1201 ctcgtagtac gggttctgag cggcgcggat cgacagctcg accgcccggc gggcgtccat
     1261 cccgctatcc agcgccaggg ccagcgacca cgtcagccgc gagagggcca gcgactccag
     1321 gcacttgccg agcacgggaa tccgcatcgc caggagcatc ggctgcggtc ccatccagcc
     1381 gcgggtgatg gccatgcaga aaagcgtgat cccgcccgca atgaaggcgc aaaacgtgaa
     1441 ccagaccagc gctccgcttg gaccgacgag gccccagccg agcacgtccg gcgcatcgcc
     1501 gccgctgacc gcgccgatga taccggtgac caggatcaag aggccgatca ccagcaccgc
     1561 gaagaccagc tcgaagagcg gccaggcgat gccgatccag aaggtccgct gcatcgtggc
     1621 caggtgctcg tagtgctcgg ccaggcggtg cagcacctcg tcgagcttgc cagtctgctc
     1681 gccgatcgcg accatttgca cgaacatcgg cgggaaatag ccgtcggcgc cctgcatgcc
     1741 ctcggccacg gtgccgccat gggcgatctg ctggcggatg ccgcccagcg cggccttgag
     1801 cggaccggtc gcatgccgct cttccatctc ccagacccgc cgcgcatcga tccccgactt
     1861 caccgccgtg ccgacgcggc ggcagagctg gatcagggag cgggtggaga tcatggggat
     1921 ttcagatttc agatttcaga attcagattg cggactcaga gtttagggtg ggtcaaccga
     1981 gtccgcgagc gcagacccat gcggcagcgg ctcggccata agggccattg taaacgaacg
     2041 gcgcgcgggg cgctccatca accgcgacca ctggtcgcgg ctttgtcagg gcgtaaatcg
     2101 ccaatcgact ttgccgcggt aggccatggt ctcttcgacg agcgcgaaga ggtcggcgtc
     2161 gtagtccttg aggagctcgc gggtgcggat cggatggggc gcgtcgttgg gggctccgtc
     2221 ctgaccgagg gcgtcgaaat aggccaaaac gccggcggcc cagtagttca ccgggctgtg
     2281 cacggccgaa gtgcccttcc atttctcggc ttcgcccgct gccttgtgca gctcggcgag
     2341 ccgttggcca aaccgctcgt ccagccggcg aacacgcagc tcgtattgtt gcacgccccg
     2401 gcggcggtcc cagttcggat cgacgggccg atttccgcag acctcataag cggcccgggc
     2461 catcaagcgg atgacctggt tgtcgccgac gttgggcagc cgcgggtcgc cgccgacgtt
     2521 ttcctcgccg acgacgatca gcttcgtctc gggcgaatag gccagcgcgc gggtgagcgg
     2581 gtcgaagtct ttcatctctt tcagcagtgc gtattcgggc aggtcggcga ttcgctcgcc
     2641 cttgccgagc accaccagct tcacgccgtc ggcgatcagc gccttgagca catcatgtcg
     2701 gtaggcgaac atcttgcgga tcgtgtcgtt ggccttcagc agcgcggcat cgctcgcccc
     2761 gcggccgacg acggtgaatt cgcgggccca ggtgaacctg gtgtagtacg ggtcaatctt
     2821 gaacttggcc ggcggcgggc cgacgttggg cagcttctgc agccaggaat agcgccagtc
     2881 ctgctcgggg ctgaggttga acgtcgtccg gaccagttcg taagcctcgg gatcgtacac
     2941 cttgagctgc tcgcgcgtgc cgatcgggtt gtggttccag ttgttgaccc gctggcagtc
     3001 gaaataggcc tggcagattt cggcccagta ctcgccggca ttgctgccgg tgtagacgtc
     3061 ctggtgcagg cccttgctgc gggcattgcg atagacatca ttcttccgct gggaccaact
     3121 cggatcgatc gaccgcagcg tgccgtcgat cgtgtggcag aactcgtgga cggcgatgct
     3181 ctcgtcgtcg tagcggtcca gcggcaggct cagcacgttc tcttcgccaa agctggtcgg
     3241 ccggccgccg gtgccgcgaa cccgctcgtt ttgatacgcg gggttgggat gtttgcgata
     3301 ttcgggcatg tccgtataga cctggtcctt gcccatcacg atcaggtaca tccggttctc
     3361 gaccagcttc gcggtgacgt cgggccggcc ggccagcatg cgggtcacga tctcgtacac
     3421 gcggaccagc gcctggtcgg ccacgtcggc gtgggccacc accggcatgc cgttcacgtc
     3481 gatgtacttc gaatagaaac cccgggcgac ttcccggtcg ccctccgcca cgatctgaaa
     3541 gaacgactcc ggcggcggcg aaaccttggg cggctcggca ttcgccgtat ccagcaggat
     3601 tccgcatacg atggccgaca gaagcgcgcg actgatcatc ggagcctccc agatgctggc
     3661 ctcgatcacc ggttaatcgt cggcgcgatg gtaatcgagc cgcccgcggg gtgcaatcga
     3721 gcggccgact acctggcctt gggcctggtg atcgtgatgt tgaagtcaga cttgggggcg
     3781 gttacatcca gctccaggcc cgacgaatcg aatcgctccc atttcttgtc gatgaagggc
     3841 ggcagcgtgc gctcctcctg ttcgctcatg gcccgcggcg cgacgagtag cttgtatttg
     3901 cccggcggca cgccgtcgcc gggttggtcc gttcccagac tgaatgagcc atccttgccg
     3961 atgtttgcct ggaccatgac cgtcttgccg ccctccagcg tggcttcgcc catcaccgtg
     4021 ccggcgtcga gcggcgtgcc gtcggagaac agaacttttc cgctgaccgg gaatcgcgtc
     4081 tggccgcaac cggggctgga gaccagcaat agacccacgg cgaggcccat caggcgcgtg
     4141 gcaatcgacc gcgaatcgct catgggat
//
LOCUS       DASRJB010000891         7440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4202292, whole genome shotgun sequence.
ACCESSION   DASRJB010000891 DASRJB010000000
VERSION     DASRJB010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4202292"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="VFB80_23485"
     CDS             complement(<1..2002)
                     /locus_tag="VFB80_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZN36820.1"
                     /translation="MLDQRRLYLDLFAVMLAAVVVFLALALVSYDPADPVAALTPPLN
                     RLYQADSLVHPPHAKAGNYCGYWGALTADLLLNLLGVGAYYLVLSLAVLDVQLLRRRE
                     IDLPALRMTGWLASLLGVTTLAAIGFPSLAPGPVIGSGGYLGALGKSLALIHFATIGG
                     LILAGSVTLGGLLLATDYAFIQVIILFFKIAKFRRSSESTEAGGKAAAAKRNRTDLDE
                     SLPGDVAVKIRGKKPAVGQTFVSAAATNSDDESDEEDESPPTLRVVNPAADKAQPATT
                     TRQDLAESPLRVKNPADRKKEAADRERQELLRRMNESAPADAEDYELPSVEMLLPSDD
                     INFEAQATEVRRKAKILEKTFLDFGFTVKVVEIETGPVIAQYEVELEAGLRLSKITGL
                     ADDLAIALRVPSVRIVAPIPGKNTVGIEVPNEQRQIVRIREVIEEANGKVKKMRIPLF
                     LGKDVSGNALTVDLSTLPHLLIAGRTGTGKSVCLNAIISSILMTRRPDEVRMLMIDPK
                     MVELSGYGRLPHLMHPVVTDMRKAEAILAWAVEKMEERYALLARAGVRHISSYNQLGE
                     EELMERLQPENDDERASIPQQLPFIVIVADEMADLMMTSGKEVEQHIIRLAQKSRAVG
                     IHLILATQKPTVDVITGLIKSNLPARIAFQVASRTDSRVVLDE"
     gene            2204..3334
                     /locus_tag="VFB80_23490"
     CDS             2204..3334
                     /locus_tag="VFB80_23490"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN36821.1"
                     /translation="MKRCALLLSLVLGGPAIVLAQNPRPLPVVSPEIEAGGRVSFRLR
                     ASEAKEVVLRGQWTREPQPMSRGEGGVWSLAAEVPAGVWEYSYQVDGLNVLDPQNPAF
                     KPQRETSKSIVHIPGSPPNPWDWQDVPHGTVHEHRYESKALGRPRELVVYTPPGYERA
                     ANPAPRYPLLVLQHGSGDNHQTWIRHGKAHWILDNLIAAGRTKPMVVVMIDGHPLGLM
                     SRDAAPQRRGAALDAFRRELLEDALPLVESLYRVQTDAANRAIAGLSMGGGQSLGVGL
                     TNLDRFAWVGAFSAAPPSAETSQAVLADPTGANSRLKLLWIACGQDDFLKVRNEEFVA
                     ALKDKNVQHQWQLTAGDHSWPVWRRYLADFAPLLFQTSARSN"
     gene            3397..4716
                     /locus_tag="VFB80_23495"
     CDS             3397..4716
                     /locus_tag="VFB80_23495"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZN36822.1"
                     /translation="MDLAWISLAALLLVMVASCTSTVNAGLLSIALAWIVGIYVAGAI
                     GQPLEMKDLVAGFPTDLCLTLIGVTLLFSQAQVNGTLERVARFAVRGCRGDLGLVPIM
                     FFVLSVVLSSVGAGNIAGTALIAPLAMTLAHRAKIPAFLMAILVAHGALAGAVSPLAP
                     TGIIADKLMHQELGLSGFENRLYLYNLAANAFVGLAGYLIFGGWRLFGRPYREEAPPS
                     ESAPATDAAASGASAAPPSEWTRAHVITLLVIGALIVGVVFFQVHVGMGAFAGAVLLT
                     LCRAADERKAMQMIPWGVILMVCGVTVLTSLLDKTGGLDRFAKLVSQVSTPQSVPGVS
                     ALLSGILSVYSSTSGVVLPALLPTVPKLLDQLGGGDPFAVACSVVVSGHVVDSSPLST
                     IGALCIASAPALEQRQTLFNQMLAWGLAMAVVGAIGCYVFFGLLGLR"
     gene            4722..5939
                     /locus_tag="VFB80_23500"
     CDS             4722..5939
                     /locus_tag="VFB80_23500"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN36823.1"
                     /translation="MTRFRVASRIAELRPTAVNRVLDEVRAYQASGRTALSLMRGQPD
                     SPTPAHIVEAAEQSLQRGRTGYPDNQGEPALRQAVADKLRRDQGLAYDPGSEILITDG
                     ATCGLALALGAVLEPGDEVLLPDPIYDAYASPIALFGGRGIPVRSIVQGGRFTLERSA
                     LEAAHSPRSRALLLNHPWNPVGTVFTSADLQTIVDFVSEHNLVLISDEIYESLVYDGR
                     RHISPASLDGARSRTILVNSLSKTYAMTGWRVGYCAAPAELTRAMLLLLQQFSRGPAT
                     FVQDAAACALLSSQESSTQRAAEYQARRDRVIRALQGIPGIRPLIPEGGLFVMVDLRE
                     LQDGVASDDVRRFLLERHGVVVIHGSAYGPGGEGLLRVSFAAGGKTLDDGLARLREGL
                     VEIASGQWHEARP"
     gene            5936..>7440
                     /locus_tag="VFB80_23505"
     CDS             5936..>7440
                     /locus_tag="VFB80_23505"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN36824.1"
                     /translation="MSARASGGGDRLSAALSAEIQGEVRFDPISQALYSTDASVYQIL
                     PLGVVIPKSREDVIRTVRACRAAGASITARGGGTSQAGQAIGAGVQLDFSKYLHKVLS
                     LEPEAQRVRVEPGIVLDELNAFLRPYGLLLPLDISTSDRATIAGMIANNSSGTRSVVY
                     GKTIDYVESLTALLSDGSVVEFSPQNSDQLDAKCAQADLEGGCYRAVRRLAREHAAEI
                     ERRYPRILRRVGGYNLDEFLPEKSPFNLSRLIVGSEGTLALILDATLRLVRPPKHRAM
                     CVVQFRDLLESLAATPLILQHQPSAVELVDRFILDSTRGKTEFEPLRSFIVDDPAAVL
                     FVEFFGETPDECAGRVAELEQALRAASLGYHVYRALEPAAQARLWKLRQAALGLSMSE
                     YGDAKAISFVEDTAVAPERLRDYIERFLQILERHGTRAGFYAHASVGLLHVRPVVNLK
                     QAEGVAKFSAIAEEISDLVLEFGGALSGEHGDGLVRSPFQQKMYGPVLYGA"
BASE COUNT         1138 a         2369 c         2569 g         1364 t
ORIGIN      
        1 tctcgtcgag caccacgcgg ctgtcggtgc ggctggcgac ctgaaacgcg atccgtgccg
       61 gcaagttcga cttgatcagg cccgtgatga cgtcgaccgt cggcttctgc gtggcgagga
      121 tcaggtggat gccgaccgcc cggctcttct gggccaggcg gatgatgtgc tgctcgacct
      181 ctttgccgct agtcatcatc aggtcggcca tttcgtcggc gacgatcacg atgaacggca
      241 gctgctgcgg gatcgacgcc cgctcgtcgt cgttctccgg ctgcagccgc tccatcagct
      301 cctcttcgcc gagttggttg tagctgctga tgtggcggac gccggcgcgg gccagcaggg
      361 cgtagcgctc ttccatcttc tcgaccgccc aggccaggat cgcctcggcc tttcgcatgt
      421 cggtgacgac ggggtgcatc aggtgcggca gccggccgta gcccgagagc tcgaccattt
      481 tggggtcgat catcagcatg cggacctcgt cggggcggcg ggtcatcagg atcgacgaga
      541 tgatggcgtt caggcagacg cttttgccgg tgcccgtgcg gccggcgatc aagaggtgcg
      601 gcagcgtcga gagatcgact gtcagggcgt tgccgctgac gtccttgccc aggaagagcg
      661 gaatccgcat cttcttgacc ttgccgttcg cctcttcgat gacctcgcgg atgcgaacga
      721 tctgccgctg ctcgttgggc acttcgatgc cgaccgtgtt cttgccagga atgggcgcga
      781 cgatgcggac gctgggcacg cggagggcga tcgccaggtc gtcggccagg ccagtgattt
      841 tggacaacct caggccggct tcgagctcga cttcgtactg ggcgatgacg ggcccggtct
      901 cgatttcgac gactttgacg gtgaagccga aatcgaggaa ggtcttttcg aggattttgg
      961 ccttgcggcg aacttccgtg gcctgggcct cgaagttgat gtcgtcgctg gggaggagca
     1021 tttcgaccga gggcagctcg taatcctcgg cgtcggccgg cgcggactcg ttcatccggc
     1081 ggagcagttc ctggcgctcg cggtcggccg cttccttctt gcggtcggcc gggttcttca
     1141 cgcgcagcgg actctcggcc aggtcttgcc gcgtggtggt tgccggctgg gccttgtcgg
     1201 cggcaggatt gacgacgcgc agcgtgggag gcgattcgtc ttcttcgtcg gactcgtcgt
     1261 cgctattggt tgcagcggca gacacgaatg tctgccccac ggcgggcttc ttgccgcgga
     1321 ttttcaccgc gacatcgccc ggcagcgatt catcgaggtc ggtgcggttg cgtttggccg
     1381 ctgctgcctt gcctccggcc tccgtgcttt cgctcgagcg ccggaacttc gcgatcttga
     1441 agaacaggat gatgacttga atgaatgcgt agtcggtcgc cagcaggagc ccgccgagcg
     1501 tgacgctgcc ggcgagaatc aggccgccga tcgtggcgaa gtgtattaaa gccagcgact
     1561 tgccgagcgc gccgaggtag ccgcccgagc cgatgaccgg ccccggcgcg agcgaaggaa
     1621 agccgatcgc ggccagcgtc gtcacaccca gcagcgacgc cagccagccg gtcatccgca
     1681 gcgcgggcaa gtcgatctcg cgccgccgca ggagctgcac gtcgagcacg gccagcgaaa
     1741 gcacgaggta ataggccccg acaccgagca ggttcagcag caaatccgct gtcaaggcgc
     1801 cccagtagcc gcagtagttg ccggccttgg catgcggcgg atggacgagc gagtcggcct
     1861 ggtagagccg gttcagcggc ggcgtaagcg cggcgaccgg gtcggcgggg tcgtagctga
     1921 ccagcgccag ggccagaaag acgacgaccg ccgccagcat gacggcgaac aagtcgaggt
     1981 agagtcggcg ttggtcgagc atcggcacgc gcggttggcg gaggtttcag ccagggcgcg
     2041 tccttgcccc acagttcccg gtttcatcga tggggatcgg cccggccgca cagcgggccg
     2101 cagttttccg gtggagggcc gcacagtttg ccgaaagcgg gccctagcgc gttaagctgt
     2161 agcgattgtt tggaaccacc accaccgaat ggcgagtact tccatgaagc gttgtgcgct
     2221 cttgttgtcg ctggttctcg gcggtcccgc gatcgtgctg gctcagaatc cgcggccgct
     2281 gccggtggtc tcgccggaaa tcgaagcggg cgggcgcgtg agcttccgcc tgcgtgcgtc
     2341 ggaggccaag gaagtcgtcc tgcgcgggca gtggacgagg gagccgcaac ccatgtcccg
     2401 cggcgaggga ggcgtgtgga gcctggcggc ggaagtgccg gcgggcgtgt gggagtacag
     2461 ctatcaggtg gacgggctga acgtgctcga cccgcagaat cccgccttca agccacagcg
     2521 cgagaccagc aagagcatcg ttcacatccc gggctcgccg ccgaatccat gggactggca
     2581 ggacgtgccg cacggcaccg tgcacgagca ccgctacgag tcgaaggcgc tcggccgacc
     2641 gcgcgagttg gtcgtctata cgccgccggg ctacgagcgc gcagcgaacc cggccccgcg
     2701 ctatccgctg ctggtcctgc agcatggctc gggcgacaac caccagacat ggatccggca
     2761 cggcaaggcc cactggattt tggataacct gatcgccgcc ggccgcacta agccgatggt
     2821 ggtcgtcatg atcgacggcc atccgctggg cctcatgtcc cgcgatgcag ccccgcagcg
     2881 gcgcggcgct gccctcgatg cgtttcgccg cgagctgctg gaggacgcgc tgccgctcgt
     2941 ggagtcgctc tatcgcgtcc agacggacgc cgcgaaccgg gcgattgccg gcctgagcat
     3001 gggcggaggc cagtcgctgg gagtgggcct gacgaacctc gaccgcttcg cctgggtcgg
     3061 tgcgttcagc gccgcgccgc cctcggccga gacttcgcag gccgtcctcg ccgacccgac
     3121 cggggccaac tcgcggctca agctgctctg gatcgcgtgc gggcaggacg actttctcaa
     3181 ggttcgcaac gaagagttcg tggcggcgct gaaagacaag aacgtgcagc atcagtggca
     3241 actcaccgcc ggcgatcact cgtggccggt gtggcggcgc tatctcgccg actttgcgcc
     3301 gctgctgttc cagacttccg cgaggtcaaa ctgagtcgtg gcaggcgcag cagctttcca
     3361 gtccaccaca aagccgtagc aggaaagtcg cgaagcatgg atctggcctg gatttcgctt
     3421 gctgccttgc tgctggtaat ggtcgcgagt tgcacttcga ccgtgaatgc cggtttgctc
     3481 tcgatcgcgc tcgcttggat cgtggggatc tacgtcgccg gggcgatcgg gcagccgctg
     3541 gagatgaagg acctcgtggc ggggtttccc acggacctgt gcctgacgct gatcggcgtg
     3601 acgctgctgt tctcgcaggc gcaggtgaac ggcacgctcg agcgggtggc gcggtttgcc
     3661 gttcggggct gtcgcggcga tctggggctc gttccgatca tgttcttcgt actgtccgta
     3721 gtcttgtcgt cggtcggggc ggggaacatc gcgggcacgg ctctgatcgc gcccctggcg
     3781 atgacgctgg cgcaccgggc gaagattccg gcgttcctga tggcgatcct ggtcgcgcac
     3841 ggcgcgctgg ccggggccgt ctcaccgctc gcgccgaccg ggatcatcgc cgacaagctg
     3901 atgcaccagg agctcgggct gtcgggtttc gagaaccggc tctacctcta caacctggcg
     3961 gcgaatgcgt tcgtcggcct ggccggctat ctcatctttg gaggatggag gttgttcggc
     4021 cgcccgtacc gcgaagaagc accgcccagc gagtctgcgc cggcgacaga cgccgccgcc
     4081 agcggcgcca gtgcagcccc gccctccgaa tggacgcggg cccatgtgat tacgctgctg
     4141 gtcatcggcg cgctgatcgt cggagtggtc tttttccagg tccacgtcgg catgggagcg
     4201 tttgccggag cagtgctgct cacgctttgt cgtgcggcgg acgagcggaa ggcgatgcag
     4261 atgattccgt ggggcgtgat cctgatggtg tgcggcgtga ccgtgctgac ttcgctgctg
     4321 gacaaaacgg gcggcttgga ccgctttgcc aagctcgtca gccaggtctc gacgccgcag
     4381 tcggtccccg gtgtgagcgc gctcttgtcg ggcatcctgt cggtctacag cagcacctcg
     4441 ggcgtcgtcc tgccggcgct gctgcccacc gtgcccaagc tgctggatca gctcggcggc
     4501 ggcgatccgt ttgcggtcgc ctgctcggtc gtggtcagcg gccacgtcgt cgattcctcg
     4561 ccgctctcca cgatcggggc gctctgcatc gcgtccgcgc cggccctgga gcagcggcag
     4621 acgctgttta atcagatgct cgcctgggga ctggcgatgg cagtcgtcgg cgcgatcggc
     4681 tgttatgtgt tctttggact gctgggtttg cgatagcaca gatgactaga ttccgcgtcg
     4741 cctctcgaat cgccgaattg cggccgaccg cggtcaaccg cgtgctggac gaagtccgcg
     4801 cgtaccaggc gagcggaagg acagccttgt cgctgatgcg cggacagccc gattcgccga
     4861 cgccggcgca tatcgtcgag gccgccgagc aatcgttaca gcgcggccgc acgggctatc
     4921 ccgataatca gggcgagccg gcgctgcggc aagcagtggc cgacaagctg cgccgagatc
     4981 aagggctggc ttacgacccg ggcagcgaga ttctgattac ggacggcgca acgtgcggcc
     5041 tcgccttggc gctgggagca gtactggagc cgggagatga agtgctgctg ccggacccaa
     5101 tctacgatgc gtatgcctcg ccgatcgcgc tgttcggcgg gcggggtata ccggttcgct
     5161 ccattgttca gggcggccgg ttcacgctgg aacgctcggc gctcgaggcg gcacactcgc
     5221 cccggtcccg cgccttgctg ctcaaccatc cctggaatcc ggtgggaacg gtcttcacca
     5281 gtgccgacct gcagacgatc gtcgatttcg tcagcgagca caacctcgta ctgatcagcg
     5341 acgagatcta tgaatcgctc gtctacgacg gccggcggca catttcaccg gcgtcgctgg
     5401 acggcgctcg ctcgcggacg atcctggtca acagcctctc caagacctac gcgatgaccg
     5461 gctggcgcgt cgggtactgc gccgcgcccg cagaactgac gcgggccatg cttctgctgc
     5521 tccagcaatt cagccgcggc ccggccacct tcgttcagga cgcggcggcc tgcgcgctct
     5581 tgtcgagcca ggagtcctcc acacagaggg cggccgagta tcaggcccgt cgcgaccgag
     5641 tcatccgggc actgcaaggc attccaggca ttcgcccgct gattcccgag ggtggcctgt
     5701 tcgtgatggt cgatctgcgg gagctgcagg acggagtggc gtccgacgat gtgcgccgct
     5761 tcctgctcga acggcacggc gtggtggtaa tccacggcag cgcttatggc cccggcggcg
     5821 agggcctgct ccgggtttcg tttgctgccg gaggtaagac cctcgacgac ggcctggctc
     5881 gcttgcggga gggcctcgtc gaaatagcga gtggccagtg gcacgaggcc cgcccatgag
     5941 cgctcgcgcg tctggtggtg gcgaccggct tagtgcggcg ctgtccgcgg agattcaagg
     6001 cgaggtgcgt tttgacccga tctcgcaggc gctctattcc accgatgcga gcgtctatca
     6061 aatcctcccg ctgggtgttg tgattcccaa atcgcgagag gacgtcatcc ggacggtgcg
     6121 ggcttgccgc gccgcgggtg ccagcatcac ggcccgtggc ggcgggacct cgcaggccgg
     6181 gcaggccatc ggggccggcg tgcagctcga tttctccaag tacctgcaca aggtgctgtc
     6241 gctcgagcct gaggcccagc gcgtccgcgt cgagccgggg atcgtgctcg acgagctgaa
     6301 tgcgttcttg cggccgtatg gactgctcct gccgctggac atttcgacca gcgaccgggc
     6361 cacgatcgcc ggcatgattg ccaacaactc gtccggcacg cggtcggtcg tgtatggcaa
     6421 gacgattgac tatgtcgagt cgctgacggc cctgttgtcg gacggctcgg tcgtggaatt
     6481 ctcgccccaa aattcggacc agctcgacgc gaaatgcgcc caggccgacc tggaaggcgg
     6541 ctgctaccgg gccgtccgcc gcctggcccg tgagcacgcc gcggagatcg agcgccgcta
     6601 tccgcgaatt ctgcggcgcg tcggcggcta caacctggac gaattcctgc ctgaaaagtc
     6661 gccgttcaac ttgtcgcggc tgatcgtcgg ctcagaaggt acactggccc tcattctgga
     6721 tgcgaccctc cgcctggttc gcccgccaaa gcaccgggcg atgtgcgtcg ttcagttccg
     6781 cgatctgctg gaatcgctgg ccgcaacgcc gctgatcctg caacatcagc cgtcggcggt
     6841 cgagctcgtg gatcgattca tcctggacag cacgcgcggc aaaacggagt ttgagccgct
     6901 gcggagcttc atcgtggatg accccgcggc ggtgctgttc gtggagttct tcggcgagac
     6961 gcccgatgag tgcgccggcc gcgtcgcgga attggagcag gccctgcgcg ccgccagcct
     7021 gggataccac gtctatcgcg ccctggagcc cgcggcgcaa gccaggctgt ggaagctgag
     7081 gcaggcggcg ctcggcctgt cgatgtccga atacggcgac gcgaaggcga tctcattcgt
     7141 cgaggacacc gccgtcgctc ccgagcggct gcgggactac atcgagcggt tcctgcagat
     7201 cctcgagcgg cacggcacgc gggccggctt ctatgcccac gcgtcggtgg gcctgttgca
     7261 cgtgcgaccg gtggtcaatc tgaaacaggc cgaaggtgtg gcgaaattca gcgccatcgc
     7321 cgaggagatt tcggacctcg tgctcgaatt cggcggggcg ctctcgggcg agcacggcga
     7381 cgggctggtc cgcagcccgt tccagcagaa gatgtacggt ccggtgcttt acggcgcgtt
//
LOCUS       DASRJB010000892         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3080496, whole genome shotgun sequence.
ACCESSION   DASRJB010000892 DASRJB010000000
VERSION     DASRJB010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080496"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1300)
                     /locus_tag="VFB80_23510"
     CDS             complement(20..1300)
                     /locus_tag="VFB80_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36825.1"
                     /translation="MNGVALLVALSALGIDHTWRSTQEGQVEYVLQVEPVFLQALIDG
                     QEIKSELPPAMQRADRICIRIGSSSLKQLDRVAPDWPDLRQPAERQATERAIEVPPAI
                     YADAAGQAYKSYEVTHGWTPAGDEESQYLLQIDPTLVKSLREGDELYAAILPEAGEVR
                     SFVITAGREDLPRQSAKPAVAVAQLQTRGAGEASADPTLTDLTQPEAGSVYGDGQASV
                     TRPPPGASAPLRSGMRPGWNISDAQDAGNQTAIPYAAGQSPATTLQPPDTSLLDVPPF
                     DHEQFSGRTTSGGTRAGTTAAGSGAAAVKNTAPRHPSQQPATSSRPNVRGQDRRIAQE
                     NDWQVEEPEARLASRTTAPPSSMIDDSPRRGTTTRSAESAADKEPQFPVLPFTLSLFA
                     LFLSLGGNLYLAWTAAEFYSRYKLAVERLRSAAR"
     gene            1416..1688
                     /locus_tag="VFB80_23515"
     CDS             1416..1688
                     /locus_tag="VFB80_23515"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZN36826.1"
                     /translation="MKVRSDVHYSGRVQGVGFRFNAQWIARGFVVTGFVRNLPDGRVQ
                     LVAEGEEKEVDRYLAKIADSMAGNISRTDARRLEASGEFSSFEIAR"
     gene            complement(1780..>2617)
                     /locus_tag="VFB80_23520"
     CDS             complement(1780..>2617)
                     /locus_tag="VFB80_23520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN36827.1"
                     /translation="KGKAVFAKRCANCHRLENAGHYVGPDLAALTDRSATAMLTAILD
                     PNRAVEDKFLDYLALTTDGRQVTGMLLAETGNSITLAGQESKQTVLLRSEIEQLKSSG
                     KSLMPDGVEKEIPLADMTDLLTYLKSIGAPPKKFPGNEPEVVRAFNDGSIRLFAENAR
                     IYGPTAIFEEKWRNVGWWQSPEDHVIWSLEVPTAGEYSVVIDYACDSGAAGDSYVIDI
                     AGQRIGAKAEATGTWDNYRGKTLGTVKLPVGPTELVVRPDGPIRSALMDLRGIRLAPV
                     SK"
BASE COUNT          421 a          834 c          861 g          501 t
ORIGIN      
        1 cgcgcggaaa ccggccagct catcgagctg cgctccgcag ccgctccacg gccagcttat
       61 accggctgta aaactcggcc gccgtccacg cgagatacag attgccgccc agcgacaaga
      121 aaagcgcaaa caaactgaga gtgaacggca gaacgggaaa ttgcggttcc ttgtcggcgg
      181 cactttcggc gctgcgggtg gttgtgccgc ggcgcggaga atcgtcgatc atactcgatg
      241 gtggagcggt cgttcgcgac gccaggcgcg cttccggctc ctcaacttgc caatcgttct
      301 cctgggcgat gcggcggtcc tggccgcgca cgtttggccg cgatgaagtc gcgggctgct
      361 gcgaagggtg acgcggggca gtgtttttga cggccgctgc gccactcccc gccgccgtcg
      421 tgcccgcgcg tgtgccgccg cttgtcgtgc gccccgagaa ctgctcgtga tcgaagggcg
      481 gcacgtccaa cagcgatgta tcgggcggct gaagcgtcgt ggccggtgac tggccggccg
      541 cataaggaat tgcggtttga ttgccggcgt cctgggcatc ggatatgttc cagcccggcc
      601 gcatgcccga ccgcaggggc gctgacgcgc caggcggcgg ccttgtcacg gacgcctgac
      661 catcgccata gaccgagccc gcttccggct gtgtcaggtc ggtgagcgtt ggatcggcgg
      721 atgcttcgcc ggcgcctcgt gtctgcaact gggccaccgc gactgccggc ttggccgatt
      781 gacgcggcag gtcctctcgg ccggccgtga tgacaaacga gcgcacctcc cccgcctcgg
      841 ggaggatcgc cgcgtaaagc tcgtcccctt cacgtagcga cttgaccagc gtggggtcga
      901 tctgcaagag atactgcgat tcctcgtcgc cagcgggcgt ccagccgtgc gtcacttcat
      961 acgacttgta tgcctgtccc gccgcatcgg catagatcgc cggcggcact tcgatcgcgc
     1021 gctcggtcgc ctgacgctca gcgggctgac gcaaatcggg ccagtcgggt gcgacgcggt
     1081 cgagctgctt gaggctgctg ctgccgatgc gaatgcagat ccgatcggcg cgctgcattg
     1141 cgggggggag ttcactcttg atttcctggc cgtcaatcag cgcctggaga aacacgggct
     1201 ccacctggag gacgtattcc acctggccct cctgcgtgga ccgccaggtg tgatcgatcc
     1261 cgagcgcgga caacgccacc agcaatgcga cgccattcat ggcttcgtgc tccttaaccg
     1321 ccgttacaat cggcggtatc ttagcggtcg gcggggaata tggtgaatag cgaaagttgg
     1381 cccgctagca gcccagcgta cgcgaggcgc acgtcatgaa agtccgctcc gatgttcact
     1441 attcgggccg cgtccagggc gtcggctttc ggttcaatgc tcagtggatc gctcgcgggt
     1501 ttgtcgttac gggctttgtg cgcaatttgc ccgacggccg cgtgcaactc gtggcggaag
     1561 gggaagaaaa agaggtcgat cgttatctgg ccaagattgc cgactcgatg gcggggaaca
     1621 tatcgcgcac ggacgctagg cggctcgaag cgagcggcga gttttcgtcg ttcgagattg
     1681 cgcggtagcg atcgttgccc ttagccgggg gctaacagcc cccggagaac gttcggcgtt
     1741 cgacgcgcat tccgggacct gtcgggtccc ggctaagaac tacttgctca ccggcgccaa
     1801 ccgaatgccg cggaggtcca tcagcgccga gcggatcggc ccatcgggac gcacgaccaa
     1861 ttcggttgga cccacgggca gcttgacggt ccccagcgtc ttgccgcgat agttgtccca
     1921 ggtgccggtc gcttcggcct tggcgccgat ccgctggccg gcaatgtcga tcacgtacga
     1981 gtcgcccgcc gcgccgctgt cgcaggcgta gtcgatcacc acgctgtact cgccggccgt
     2041 cggcacttcc agcgaccaga tcacgtggtc ctccggactc tgccaccagc cgacgtttcg
     2101 ccacttctcc tcgaagatgg cggtcggccc gtagatccgg gcgttctcgg caaacaggcg
     2161 gatcgagccg tcgttgaagg cccgcacgac ctccggctcg ttgccgggga attttttcgg
     2221 cggtgcgccg atcgacttga gatacgtcaa gaggtcggtc atgtcggcca gcgggatttc
     2281 cttttccacg ccgtcgggca tcagcgactt gccgctgctc ttgagctgct caatctcaga
     2341 gcgcagcagc accgtctgct ttgattcctg gccggcgagc gtgatgctgt tgcccgtttc
     2401 ggccaggagc ataccagtga cctggcggcc gtcggtggtc agcgccagat aatcgaggaa
     2461 cttatcttcc accgcccggt tcggatcgag gatcgccgtg agcatggcgg tcgccgagcg
     2521 gtcggtgagt gccgccagat cgggaccgac gtagtggcca gcattttcca gccgatggca
     2581 gttggcgcaa cgcttggcga agacggcttt tcccttc
//
LOCUS       DASRJB010000893         5809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2234608, whole genome shotgun sequence.
ACCESSION   DASRJB010000893 DASRJB010000000
VERSION     DASRJB010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234608"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFB80_23525"
     CDS             complement(<1..579)
                     /locus_tag="VFB80_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36828.1"
                     /translation="MSRFVMALTCWLAATALAQEQPEKAKPADPPAKTEKSDKAEKSD
                     KTPAEQFAQLYGEWSALDKRLNELQDSYATAAPAAREEIKKQYTELVTRSTEMIPKLR
                     VAAEAAYVAAPNKDADVTKTLIGIVAYDVRRDEYESALKLAKLLEENKCDEAVLYGVA
                     GQAAYSLDDYETAEKYLAIADKAGKLDREGQNM"
     gene            complement(693..1214)
                     /gene="tpx"
                     /locus_tag="VFB80_23530"
     CDS             complement(693..1214)
                     /gene="tpx"
                     /locus_tag="VFB80_23530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZN36829.1"
                     /translation="MSRPGAVTFKGTPLTLAGEAVKVGQPAPDFTLHYFEGGLKTIKL
                     ADLKGKPSFISVVPSLDTPVCQIQTKKFNEQLAALGSKINAVTVSLDLPFAMNRFCGA
                     ESIKNMRCGSDYQDRSFGKSYGMLIEELKLLARGVIILDKDGKVVYAETVKEVASEPN
                     YDAALAALKSVAA"
     gene            1451..1831
                     /locus_tag="VFB80_23535"
     CDS             1451..1831
                     /locus_tag="VFB80_23535"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN36830.1"
                     /translation="MLLEVAPEWSLEIDRGPDWMFIRLQPPHAGDTGEIALAEMIWEK
                     LEQSFCHRVVLEMEQVKFLRSWMVGQLVRLHKRISAEGGMLRLSGLSPANYDVLRICR
                     LADHFPAYKNRTDAVMGYRPAKPR"
     gene            complement(1897..2118)
                     /locus_tag="VFB80_23540"
     CDS             complement(1897..2118)
                     /locus_tag="VFB80_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN36831.1"
                     /translation="MHTMEPSLAERVDQAIQTIPYVSGRTLRFETDGSRVTLQGCVKS
                     YFHKQMAQEVVRRVAGVEEVENCLEVMWV"
     gene            complement(2505..2786)
                     /locus_tag="VFB80_23545"
     CDS             complement(2505..2786)
                     /locus_tag="VFB80_23545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36832.1"
                     /translation="MKKFALALGFLSLTAFGCTTETPTPTPPTVDAAADAAKAAGDAA
                     AEAAKTAGDAAANAAKATGDAAAEAAKNAGDAAKAAIEGAKGAPEAPKP"
     gene            complement(2973..>5809)
                     /locus_tag="VFB80_23550"
     CDS             complement(2973..>5809)
                     /locus_tag="VFB80_23550"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN36833.1"
                     /translation="AAGVLFREGQLYLTCIPHLWLLKDADGDGRAETRNSLSEGYGVR
                     FAFRGHDMHGLIVGPDGRLYFSIGDRGLNVKQGDRQLVNSESGAVLRCEPDGSNLELF
                     ATGLRNPQELAFDDFGNLFTGDNNSDSGDRARWVHVVQGGDSGWRMAYQYLSDRGPFN
                     REKIWQPYDPVGNALRGVPEQPAYIVPPVANFASGPSGLAYYPGTGLPEHFKNRFLLV
                     DFRGGPANSGVRSLRVKPKGATFELVDSEATIWRVLATDVDFGPDGAIYLSDWVNGWD
                     GEGKGRIYRFAPTDAAIRDAARETQQLLTEDFSKRSADELIRLLAHSDRRVRQESQFA
                     LVGKREAAALTMAAKTNDNQLARLHALWGLSQLQRQGGLPAQLIAHVLPLLDDPDAEI
                     RAQSAKMLGEASDIAAADMLIAHLKDDSPRVRSFAAQSIANLGQPRAIEPLLKLLEDN
                     ADKDPVLRHAAVMGLVGSAGRSSEALVAQAKHPSPSARLGVLLALRRLGAAEVSQFLN
                     DADPWIVVEAARAIHDVPIPAAMPKLAAFLSRGSRDEAVMHRVLNANYRAGTPEAAQA
                     IAAYAARGDVPTNLRVEALEMLGRWEKPSGRDRVLGMWRPLPDRPAADAAAALRANLA
                     GIFKGSDRVRERAGQSAATLGIKEVIPELRTLLADTSQPAAARAGVLSALVTLNDEGV
                     EAAARGVLSDKEPAVRAAARTTLAQGGAEDAVALLTAAAEQGERVERQAALAALGSLK
                     AEAAGAALARGLDQLLAGSYPADSRLDLLTAAAKFGGDSLQQKLAEFEGRRAKDDPLS
                     PFAECADGGDATRGRQLFFERSQLSCVRCHKIGETGGDVGPELTRIAADKKRDYLLES
                     LVAPSKTIAKNFESVVVLDIDGVQHTGIVKQEDGQKLTLMTPEGRLVTIAQDRIDGRK
                     PGQSSMPEDLLKYVTKAELRDLVEFLATLK"
BASE COUNT          933 a         1897 c         1923 g         1056 t
ORIGIN      
        1 catgttctgg ccctcgcggt ccagcttgcc cgccttgtcg gcgatcgcca ggtatttctc
       61 ggccgtttca taatcgtcga ggctgtaggc cgcctggccg gcgacgccgt acagcacggc
      121 ctcgtcgcac ttgttttcct cgagcagttt cgccagcttc aatgcgctct cgtactcgtc
      181 gcggcggacg tcgtaggcca cgataccgat cagcgtcttg gttacgtccg cgtccttgtt
      241 cggcgcggcg acgtaggccg cttctgccgc cacgcgcagc ttcggaatca tctcggtcga
      301 gcgggtgacc agctcggtgt actgcttttt gatctcttcg cgggcggcgg gcgcggcggt
      361 ggcgtaactg tcctgcagct cgttcagccg cttgtcgagc gcggaccact cgccatacag
      421 ctgcgcgaac tgctcggccg gagtcttgtc gctcttttcc gccttgtcgc tcttctctgt
      481 tttggcgggt ggatcggccg gcttggcctt ctccggctgc tcctgtgcca gagcagttgc
      541 tgccagccaa caagtcagtg ccatcacgaa gcgggacatc tcacaactcc ctggaaaggc
      601 gagcgggaag cgtcagcttc ccggtcattc agcacgacga aaatggtgag tcatcgggag
      661 gctgacgcct cccgctcgcc gcgctcgcga ggctaggccg cgacgctctt gagcgccgcc
      721 agggccgcgt cgtagttcgg ctcgctggcg acttccttga cggtctcggc ataaacgacc
      781 ttgccgtctt tgtcgaggat gatcacgccg cgggccagca gcttcagctc ctcgatcagc
      841 atgccgtagc tcttgccgaa gctgcggtcc tggtagtcgc tgccgcagcg catgttcttg
      901 atgctctcgg ccccgcagaa gcggttcatg gcgaacggca ggtcgaggct gaccgtcacc
      961 gcgttgatct tgctgcccag cgcggccagt tgctcgttga acttcttggt ctgaatctgg
     1021 cagacgggcg tatcgagcga cggcaccacg ctgatgaacg acggcttgcc cttgaggtcg
     1081 gccagcttga tcgtcttcag gccgccctcg aagtagtgca gcgtgaagtc cggcgcgggc
     1141 tggccaacct tcacggcttc gccggccagc gtaagcgggg ttcccttgaa agtgacagca
     1201 ccaggacgcg acatggcatt ttcctcgctg aaaatgggca gttttgcgtg aaatcgggcg
     1261 aaaggtcgtc tcgtagtatt gtgctggcac agactgccgg caaggggcgg gcgcgctcgg
     1321 cgcggcgctc cggcagcgtt ttcctgctgc gccattgcgg tttgattgtc ccggcgtatc
     1381 atcaactgcg gggatggtgc gggtgggttt ctggcggccc agttctgggc tgccgcacag
     1441 gaggcggatc atgttgctcg aagtcgcgcc ggagtggtcg ctcgagatcg accgcggacc
     1501 cgattggatg ttcattcgcc tgcagccgcc gcacgccggc gacaccggcg aaatcgcgct
     1561 ggccgagatg atctgggaaa agctcgagca gagcttttgc catcgggtcg tgctggagat
     1621 ggagcaggtg aagttcctgc ggagctggat ggtcggtcag ctcgtccgcc tgcacaagcg
     1681 gatttcagcc gagggcggca tgctgcggct cagtggcctt tcgcccgcca attacgacgt
     1741 gctgcgaatc tgccggctgg ccgaccactt tcccgcgtac aagaaccgca ccgacgcggt
     1801 gatgggctat cggccggcga agccgaggta agcaaatacg gaatgcggaa tgcggaattc
     1861 aactccgcat tccgcactcc gaattccgca ttgaaatcac acccacatca cttccaggca
     1921 gttctcgact tcttcgacgc ccgcgacgcg gcggacgact tcctgcgcca tctgcttgtg
     1981 gaagtacgat ttgacgcagc cttgcagcgt gacgcggctg ccgtcggtct cgaagcggag
     2041 cgtgcgcccg ctgacgtacg gaatggtctg aatcgcctga tcgactcgct cggcgagcga
     2101 gggctccatc gtatgcatgt taaaccgatc ccgaaaggtg ttgacgacgt cggctctccg
     2161 tgcagcaccg gtcgcggtcg gcagggcccg cgcgttcctc acggtgctct ttcgaccgcc
     2221 ggcccaatct cgatccaagg aattagacca aggcggcgca atttgggaaa aatcgccggc
     2281 ctgtgccgaa tctgacggca gtgctagcga tgcggcctgc gtaagcgcct cgggcggcca
     2341 acgacgacta cgtcttgaac gaagagacag ttcaaagttt agccgctgac tcgcaatgcg
     2401 gcgaggtggc aggcgccagc ggcgcgcaaa aaaagagcga cgccttgggc atcgctcttc
     2461 agcgtttcac gaactggcgc ctgagggttt gaaccctccg caagctacgg cttgggggct
     2521 tcgggggcgc ccttagcgcc ttcgatggcg gccttcgcgg cgtcgccggc gttcttggca
     2581 gcttcggcgg cggcgtcacc ggtggccttg gcagcattcg cagcggcgtc gcctgcggtc
     2641 ttggcagctt cggcggcggc atcgcccgcg gccttggcgg catccgcggc agcatcgacg
     2701 gtcggcggcg tgggcgtcgg cgtctccgtg gtgcagccaa aagccgtcag cgaaaggaac
     2761 cccaaggcca gagcaaactt cttcatcaca aagtcctcaa cagagagtta acaatgcgcc
     2821 ctcaacagcg agagcggcga gtgggcagcg catcatctgt cccacacaca tagagcaccg
     2881 cttgcgggga aatattcccg cgatgcggct gccgcccaga aagtcataag ctattgtggc
     2941 aaaaggattt gcgacgctcg gtggcctcgc ggtcacttta gggtcgccag aaactcgacc
     3001 aggtcgcgga gctccgcctt cgtgacgtac ttgagcagat cctcgggcat cgagctctgg
     3061 ccgggcttcc tgccgtcgat gcggtcctgg gcgatcgtga ccagccggcc ttcgggggtc
     3121 atgagcgtca gcttttgccc gtcttcctgt ttgacgatgc ccgtgtgctg cacgccgtcg
     3181 atgtcgagaa ccacaaccga ttcgaaattc ttggcgatcg tcttgctggg ggcgacgaga
     3241 gactcgagca gatagtcgcg ctttttgtcg gctgcgatgc gggtcaactc cgggccgacg
     3301 tcgcctccgg tctcgccgat cttgtggcag cggacgcagg agagctgcga gcgctcgaag
     3361 aacagctgcc ggccgcgcgt ggcatcgccc ccgtcggcac attccgcaaa aggcgaaagc
     3421 gggtcgtcct tcgcgcggcg gccctcgaac tcggcgagct tctgctggag cgaatcgccg
     3481 ccgaacttcg cggcagcggt cagcaggtcc agccgggagt ctgccggata cgagccggcc
     3541 agcagctggt cgagtccgcg cgcgagcgcg gcgcccgccg cttcggcctt gagcgaaccc
     3601 agcgcggcca gcgcggcttg acgctcgacc cgctcgccct gctccgcggc tgcggtcaac
     3661 agcgcgacgg cgtcttcggc gccgccctgg gccagagtcg tgcgggctgc ggcgcggacg
     3721 gccggctcct tgtcgctcag gacgccgcga gcggcggcct caaccccctc gtcgttcaac
     3781 gtgactagag ccgaaagcac gccggcccgc gcggcggcgg gctgcgacgt atcggctagc
     3841 agcgtccgca gctcgggaat cacctcctta atgcccagcg tcgctgcgga ctgtccggcc
     3901 cgctcgcgga cgcggtcgga gcccttgaag atgccggcca ggttcgcccg cagggccgcc
     3961 gcggcatcgg cggccgggcg atcgggcagc ggacgccaca tgcccaacac gcggtcgcgg
     4021 ccggacggct tttcccagcg gcccaacatc tccagagcct ccacccgcag attcgtcggc
     4081 acgtcgccgc gggcggcgta cgccgcgatc gcctgggccg cctcgggcgt gcccgcccgg
     4141 tagttggcgt tcagcacgcg gtgcatcacc gcctcatcgc ggctgccccg gctgaggaat
     4201 gccgccagct tgggcatcgc cgcgggaatc ggcacgtcgt ggatggcccg cgcggcctcg
     4261 acgacgatcc acggatcagc gtcgttgaga aactgcgaga cctctgccgc gccgagccgc
     4321 cgcagggcca gtagcacgcc gaggcgggcc gacggcgagg gatgtttggc ctgcgccacg
     4381 agcgcctccg acgaacggcc ggccgaaccg acgagcccca tcaccgcggc gtgccgcagc
     4441 acgggatcct tgtcggcgtt atcttccagc aatttcagca gcggctcgat ggccctgggc
     4501 tgcccgaggt tggcgatcga ctgcgccgcg aaggaccgga cgcgcgggct gtcatccttc
     4561 aggtgggcga tcagcatgtc ggccgcggcg atatcgctag cctcgccgag catcttggcc
     4621 gactgggcgc gaatttcggc gtcggggtca tccagcagcg gcaatacgtg ggcaatcagc
     4681 tgggccggca agccaccctg gcgctggagt tgcgacaatc cccacagggc atgcaggcga
     4741 gcgagttggt tgtcgttcgt cttggcagcc atcgtcaatg cggcagcttc tcgcttgccg
     4801 acaagcgcga attgcgattc ctggcggacg cgacggtcgg aatgggcgag caacctgatc
     4861 agctcatccg ccgagcgctt cgaaaagtcc tcggtcaaca gctgctgcgt ttcgcgagca
     4921 gcgtcccgaa tcgcagcgtc ggtcggcgcg aacctgtaga tgcggccctt gccttcgccg
     4981 tcccagccgt tcacccagtc gctgaggtag atcgcgccgt cggggccgaa gtcgacgtcg
     5041 gtcgccagca cccgccagat cgtcgcttcg gaatcgacga gctcgaacgt cgcccctttg
     5101 ggtttgaccc gcagcgagcg gacgccgctg ttggccggcc cgccgcgaaa atcgaccagc
     5161 aggaagcggt tcttgaaatg ctcgggcaag cccgtgcccg gataataggc cagccccgac
     5221 gggccgctgg caaagttggc caccggcgga acgatgtacg ccggctgctc gggaacgcca
     5281 cggagggcgt tccctacagg atcgtacggc tgccagatct tctcgcggtt aaacggtcca
     5341 cggtcgctca agtactgata ggccatccgc cagccgctgt cgccgccttg cacgacgtgg
     5401 acccagcggg cccggtcgcc gctgtcgctg ttgttgtcgc cggtgaagag gttcccgaaa
     5461 tcgtcgaagg ccaattcctg cggattgcgc aggccggtgg caaacagctc caggttcgag
     5521 ccgtccggct cgcagcgcag gaccgcgccg gattcggaat tgaccagctg gcgatcgcct
     5581 tgtttcacgt tgaggccgcg gtcgccgatg ctgaaataga gccgtccgtc ggggccgacg
     5641 atcaggccgt gcatgtcgtg gccgcgaaag gcgaaccgca cgccgtagcc ctcgctgagc
     5701 gagttgcgag tctcggcccg gccgtcgccg tcggcgtcct tcagcagcca gagatgcggg
     5761 atgcacgtca ggtagagctg cccctcgcga aacagcacgc cggcggcgg
//
LOCUS       DASRJB010000894         8214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3643980, whole genome shotgun sequence.
ACCESSION   DASRJB010000894 DASRJB010000000
VERSION     DASRJB010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643980"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFB80_23555"
     CDS             <1..668
                     /locus_tag="VFB80_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZN36834.1"
                     /translation="DSDTMQRPGSHEAALSRFKSGEVKILLGTQMIAKRLDFPNVTLV
                     GVVNADTTLHFCDLRAAERTFQLVTQVAGRTGRGDKGGRVLVQTFSPDYYAILAAIDH
                     DYARFAAAELPSRRAHLYPPFASLIRLIVRGESEPAVDQFAESACEKLRAAIEAAAIE
                     HRVLGPAPCPIAKLRGLFRYHILLCSPATDELRAAVRSVTERFEPIEGLQWVIDVDPL
                     DLL"
     gene            781..945
                     /locus_tag="VFB80_23560"
     CDS             781..945
                     /locus_tag="VFB80_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36835.1"
                     /translation="MIRLQETIDRLIRGERDPEAMRKACEEMDAAREELRRRIGTVDV
                     AVDLIRDARK"
     gene            942..1358
                     /locus_tag="VFB80_23565"
     CDS             942..1358
                     /locus_tag="VFB80_23565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN36836.1"
                     /translation="MKYVLDASVALKWVLPEADAAKSLSLRDDYLNGIHSLLAPDTFA
                     VEVAHGLTRAERRGIIAVSDAVPRLQNVLATVVDLHPFLPLLSRAVALSSLHRIGVYD
                     CLYIALAEREQCKLVTADQRLMSLAHPQIVLLGNLP"
     gene            complement(1368..2696)
                     /locus_tag="VFB80_23570"
     CDS             complement(1368..2696)
                     /locus_tag="VFB80_23570"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36837.1"
                     /translation="MAANCKSFSRRDFFRLAGASVGGASASGWLSVLAGHAAEAVAAG
                     KKHKSCILLYMSGGASHIDTFDLKHGGETGTEFQAIATSVPGIQISEHLPKVARLMHH
                     GAIIRSMNTVESDHDRGRYLMHTGFSRSNGGVPYPSLGALVSSELGESGFLLPNFVVC
                     NLHDRLDPAFTGYLPPQHRGLILPDLDKGIQDIQPAVSQSELKGRLDLLGQLDAAFQG
                     KYHAPQAAAHRANYGRSYDLMRAEQLKAFDLSLEPAASHSRYTPQQFDPRNSQQNGLR
                     DFGKGCLLARRLIEVGVPFVEVVLQSWDTHQNNFPRTRQLCEALDAPWSALIEDLKDR
                     GLLDSTLVIWMGEFGRTPRINPSGGRDHFGRAWSTVLMGGGIKGGQVVGRTDPTGGTV
                     EDRPVSAGDFFATICQALGINPDKNNDGPGGRTVRITAKGAQPVLELFPG"
     gene            complement(2745..5063)
                     /locus_tag="VFB80_23575"
     CDS             complement(2745..5063)
                     /locus_tag="VFB80_23575"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36838.1"
                     /translation="MRKTWTKLVALGIAMLLALSGEALAQRGGLRPANPQTKGATEAP
                     AATGKVVAYEAGKSIALETTNRNGMQRREFKIDKDQTKIELPPRIKEIAVGQTLSVWA
                     LKETPDVAARIGTGGPAAPVNPANPRRPNRGQTPAGPPIVAGQVVAYEADKSLTIEVR
                     QRGGQVQRSEFAIANDQTKVEFVGEAKALAVGIPVTVTPDKDNAKLAARIVAGAAMNV
                     RPQRGNRPNPNPNPPNAPAPEAARPPVTVRPPRPAVTGLAPRSVAEQIDRLIHERLAA
                     EKIPASPPADDAEFIRRLYLDVVGVIPPTEKVVAFISNRESDKRARLIDELLASEDYG
                     RHFADVWCDRINVKDLPIYREPFCDWMSASLNAGRGWDEIVLDMLTAEGSFNFITRGR
                     RLGSTEPQALFLLLNTEEGQGKGPNPAWLAGESGRLFLGVQLQCAECHDHPFTASWKQ
                     TDFWGLAAFYSGLKAERPQQQQGLRWVETPATEAVNISIPATALKNVGQTVPARLLGS
                     AGDYKAADSELLRQSLARWMTAADNPYFPKATANRVWAHFFGRGLINPVDDIRPDNPC
                     SHPEILQLLGDEAKRSQFDVKHLIRCLCLTAAYQRTSVPVAGNQDDAAYYSHVAVKTM
                     GPGVFYDSLKAATGWAELKLGLPERKTKLTVLTQFTPREVFVDFFRAAQGEEASPLDN
                     DHGIPQALKLMNAAQLSGPGPVVQRLAAAGVNREQALEQLYLTALARPPSSAESALVA
                     EFLAQRNDASPEQGYAAVLWTLINSAEFVSNH"
     gene            complement(5094..5762)
                     /locus_tag="VFB80_23580"
     CDS             complement(5094..5762)
                     /locus_tag="VFB80_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36839.1"
                     /translation="MIARTTLCVLLLTWLVSAADAQQRGDTGGGLRGQPRPANKRNPT
                     PRDAEAAPPAGMRLPKTAPRSITFTPEREAAALAFVATHRPELPPVLEDLKARQPSEY
                     QRAICDFFWVSETLAAMRQADPQRHDLALRTWRLETLTHLLTSQLAGRPADAERLRTE
                     LAQAVQQLVDVQIETSAYDLKRQEAQLRRAQERHQRLADRRNDLVRERLGALEQAIEQ
                     TTAP"
     gene            complement(5759..6283)
                     /locus_tag="VFB80_23585"
     CDS             complement(5759..6283)
                     /locus_tag="VFB80_23585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36840.1"
                     /translation="MSEERDELDLLAFRYVAGELTPAEAAAFEPRLADDQAAQVAVSR
                     AVGLSQRLASAAPPADQAAPARKSALVWAQPLGWMAIGAASAVLAVNLFWRPAADRNA
                     SPATNVQPAGGSPVDALVWARLQSEQEWTAADLEDWLDQPESPDDSEEPPPAPEVPSW
                     VFATKRPSKGGVNP"
     gene            complement(6286..6813)
                     /locus_tag="VFB80_23590"
     CDS             complement(6286..6813)
                     /locus_tag="VFB80_23590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN36841.1"
                     /translation="MQQPDASAELAESNAALAELFREQRLPLQQFLLGMLRNRAEADD
                     ALQQVFLKLVENWATLRLDTAKSWLFTVAYHEAMAARRRRFRQESALAELWSRPVWQS
                     QASPPDAAERREEIEIVRSSLDALPAAQREVVERRMFRNQTFQAIANELGCPLGTVLT
                     RMRLALKALGELLEE"
     gene            6895..8031
                     /gene="solA"
                     /locus_tag="VFB80_23595"
     CDS             6895..8031
                     /gene="solA"
                     /locus_tag="VFB80_23595"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZN36842.1"
                     /translation="MTYDVIILGTGGVGSAAAFHAARRGAKVLGIDRFPGGHDRGSSH
                     GETRIIRQAYFEHPDYVPLLLRAYELWRELEPIAGEGLLHEVGLLQVGPPEGTVVRGV
                     LEAARLHGLSVDSLNGDEVQRRYSGFRVPQDCVGVFEPAAGYLKVERCVLAHLDAAEL
                     HGAEFQFGSAAVSRQTSGSDIVVTTESGKSFRAKKLIVTAGAWAPQLLAELNVKLFVR
                     RKHLYWYPTAAPEYRQDRGCPTFLYELPQGVYYGFPQIDTLGVKVAEHSGGAVITDPL
                     NDPRELDAADLARVEAFLAAQMPGVTRQLGHCCVCFYTMSPDEHFIVDRSPRDPHVLF
                     AAGLSGHGFKFTSVLGEALAELALDGSTDLPIDFLSLARFANDK"
BASE COUNT         1294 a         2683 c         2725 g         1512 t
ORIGIN      
        1 tggacagcga cacgatgcag cggcccggca gccacgaggc ggcgctgtcc agattcaaga
       61 gcggcgaggt gaagatcctg ctggggacgc agatgatcgc caagaggctc gattttccga
      121 acgtgacgct ggtcggcgtg gtcaacgcgg atacgacgtt gcatttttgc gacctgcggg
      181 cggccgagcg gacgtttcag ctcgtgacgc aggtcgccgg ccgcaccggc cgcggcgaca
      241 agggaggccg cgtgctcgtg cagacctttt cgcccgacta ctacgcgatc ctggccgcga
      301 tcgaccacga ctacgcccgc ttcgccgccg ccgagctgcc ctcgcgaagg gcgcaccttt
      361 atccgccgtt cgcctcgctg atccggttga tcgtccgcgg cgaaagcgag ccggccgtgg
      421 accagttcgc cgaatcggcc tgcgaaaagc tccgcgcggc gatcgaagcc gccgcgatcg
      481 aacaccgcgt tctcggcccg gctccgtgcc cgattgccaa gctccgcgga ttgttccgct
      541 atcacatcct gctgtgcagc cccgccaccg acgagctccg cgccgcggtt cgcagcgtca
      601 ccgaaagatt cgagccgatc gaagggctgc agtgggtgat cgatgtcgat ccgctggatt
      661 tgctgtaacc aatggcggcg gcaatcgcac ttctcaggcc gtttcgcgcg gctttcgatt
      721 gaccgcgact gaatttcgtt gaacaattca agtatgacca ctgcgatttc atcggacgca
      781 atgatcaggc tccaagagac gatcgatcgc ctgatacgcg gcgagcgcga cccggaggcg
      841 atgcgcaagg cctgcgaaga aatggacgcc gcgcgcgagg agctgcggcg gcgcattggc
      901 acggtcgatg tcgcggtcga tctgattcgt gatgcccgca aatgaagtat gtgctcgacg
      961 cctcggtcgc gctcaagtgg gtcttgcctg aggcggatgc cgcgaaatca ctttcgctgc
     1021 gggacgacta cctgaatggc attcattccc tgctcgcacc ggacacattc gctgtcgaag
     1081 tcgcacacgg cctgacacgg gcagaacgtc gcggaattat cgcagtttct gatgccgtcc
     1141 cgcggctgca aaatgtgctt gcgacggttg tcgatttgca tccctttctg ccattgcttt
     1201 cgcgtgccgt cgcgctttcc tccctgcacc gcatcggcgt ttacgattgc ctctacatcg
     1261 cgcttgcgga acgcgaacag tgcaagctcg tcacagccga ccagcggctg atgtcgctcg
     1321 cgcatccgca gattgtgctg ctcggcaacc tgccgtgatt cacgaatcta gccgggaaac
     1381 aattccagaa ccggctgagc acccttggcc gtgattcgca cggtccgccc gccagggccg
     1441 tcgttgttct tgtccgggtt gatgccgagg gcctggcaga tcgtagcaaa gaaatcgccg
     1501 gcggaaaccg ggcggtcctc gaccgtgccg ccggtcgggt cggtgcggcc gacgacctga
     1561 ccgcccttga tgccaccgcc catcaggacc gtgctccagg cgcggccaaa gtggtcgcgg
     1621 ccgccactgg gattgatccg gggcgtgcgg ccgaattcgc ccatccagat caccagcgtg
     1681 ctgtccaaca ggccgcggtc cttgaggtcc tcgatcaggg cgctccaggg cgcgtcgagc
     1741 gcctcgcaga gctgcctggt gcgggggaag ttgttctggt gcgtgtccca gctttgcagc
     1801 accacttcga cgaacggcac gccgacttcg atcagccggc gggccaagag gcaccctttg
     1861 ccgaaatcgc ggaggccgtt ttgctgggaa ttacgcggat cgaattgctg cggcgtgtag
     1921 cggctgtgcg acgcggccgg ctccagcgag aggtcgaacg ccttgagctg ctccgcccgc
     1981 atcagatcgt acgagcggcc gtagttggcc cggtgagcgg ccgcctgtgg cgcgtggtac
     2041 tttccttgga acgcagcatc caattgaccc agcaagtcga ggcgaccctt cagttcagac
     2101 tgcgaaaccg caggctggat gtcctgaatg cccttatcca ggtccggcag gatcaggccc
     2161 cggtgctgcg gcggcaggta gcccgtgaat gccgggtcca gccggtcgtg caggttgcag
     2221 accacgaagt tcggcagcag gaagccgctt tcgcccagct cggacgagac gagtgcgccg
     2281 aggctcggat aaggcacgcc gccgttggag cgcgagaagc cggtgtgcat caggtagcgg
     2341 ccgcggtcgt ggtccgactc gaccgtgttc atgctgcgga tgatcgcccc gtggtgcatc
     2401 agcctcgcga ccttgggcag gtgctcgctg atctgaatgc cggggaccga cgtcgcaatc
     2461 gcctggaact cggtgccggt ttcgccgccg tgcttgaggt cgaatgtgtc gatgtggctc
     2521 gccccgccgc tcatgtagag caggatgcag gacttgtgct tctttcccgc tgccaccgcc
     2581 tcagccgcat ggccggcgag caccgacaac cagccggagg cgctggcgcc gccgacgcta
     2641 gcgccggcca ggcggaaaaa gtcgcgacgg gaaaacgatt tgcagttcgc agccatgaca
     2701 cgctccttta ccactcacca cctaccactc aacactcact ccgctcagtg gttgctgacg
     2761 aactccgcgc tgttgatcag cgtccacagc acagctgcat aaccctgctc cggcgaagcg
     2821 tcgttccgct gggcgaggaa ttcggccacg agcgcggatt cggcggacga gggcggccgg
     2881 gccagcgctg tcaggtacag ctgctcgagg gcctgctcgc ggttgacgcc cgcggctgcc
     2941 aaacgctgca cgaccgggcc cgggccgctg agctgggctg cgttcatcag cttgagggcc
     3001 tgcgggatgc cgtggtcgtt gtcgagcggg ctggcctcct cgccctgagc ggcgcggaag
     3061 aaatcgacaa acacctcgcg gggcgtgaac tgcgtcagca cggtcagctt ggtcttccgc
     3121 tccggcaggc cgagcttcaa ctcggcccag ccggtggccg ccttcagcga gtcgtagaac
     3181 acgcccggtc ccatcgtctt gacggcaacg tggctgtagt acgctgcgtc gtcttggttg
     3241 ccggccacag gcacgctcgt ccgctggtat gccgcagtga ggcacaggca gcggatcagg
     3301 tgcttgacgt cgaactgcga gcgcttcgcc tcgtcgccca gcagctgcag aatttccggg
     3361 tggctgcacg ggttatcggg gcggatgtcg tccacggggt tgatcagccc ccggccgaaa
     3421 aagtgggccc acacgcggtt ggcggtcgcc ttgggaaagt acgggttgtc cgcggccgtc
     3481 atccagcggg ccagcgactg ccgcagcagc tcgctatctg ccgctttgta gtcccccgcc
     3541 gagccgagca gccgggcggg gaccgtctgg ccgacattct tcagcgcggt cgcgggaatg
     3601 gagatgttga ccgcctcggt ggctggtgtc tcgacccagc gcagtccctg ctgttgctgt
     3661 ggccgctcgg ccttgagccc gctatagaac gcggccagcc cccagaagtc ggtttgcttc
     3721 caggaagcgg tgaacgggtg gtcgtggcac tcggcgcact ggagttgaac gccgaggaac
     3781 agccggccgc tctcgccggc cagccaggcc ggattcggtc ctttgccttg tccttcttcg
     3841 gtgttgagca gcaggaacag cgcctgcggc tcggtcgagc ccaaacgcct gccacgggtg
     3901 atgaagttga agctcccttc ggccgtcagc atgtccaaga cgatctcgtc ccagccgcgg
     3961 ccggcgttca ggctggccga catccaatcg cagaacggct cgcgataaat gggcaggtcc
     4021 ttgacgttga tgcgatcaca ccacacgtcg gcgaaatgcc ggccgtaatc ctcgctggcg
     4081 agcaattcgt cgatcagcct ggcccgcttg tcgctctcgc gattgctaat gaaggccacg
     4141 actttctcgg tcggcggaat cacgcctacg acgtcgaggt agagccggcg gatgaactcg
     4201 gcatcgtccg caggaggcga ggcgggaatc ttttccgccg ccagccgctc gtgaatgagc
     4261 cggtcgattt gctccgcgac cgagcgcggc gcgaggccgg tcaccgccgg ccgcggcggg
     4321 cgaacggtga ccggtggccg ggcggcttcc ggcgcggggg cgtttggcgg attcggattt
     4381 ggattgggac ggttgccccg ctgcggccgc acgttcatcg ccgctccggc gacgatgcgg
     4441 gccgcaagtt tggcgttgtc cttatcggga gtcaccgtga cgggaatgcc gacggcgagg
     4501 gccttggctt cgccgacgaa ttcgactttc gtctggtcgt tcgcgatggc aaactcgctg
     4561 cgctggacct gcccgccgcg ctgacggact tcgattgtga gggacttgtc ggcttcgtac
     4621 gcgacgacct gcccggccac aatcggcggg ccggccggcg tctggccgcg attggggcgg
     4681 cgcggattgg cggggttgac gggtgcagcc gggccgccgg tcccgattct ggccgcaacg
     4741 tccggcgtct ctttgagggc ccacaccgac agcgtctggc ccacggcgat ttccttgatc
     4801 cgcggtggga gctcgatctt cgtctggtcc ttgtcgatct tgaactcccg gcgctgcatg
     4861 ccgttgcggt tggtcgtttc cagcgcaatc gacttgcccg cttcgtaggc gaccacctta
     4921 cccgtggcgg ccggcgcttc ggtcgcgccc ttcgtctgcg ggttggcagg tcgcagcccg
     4981 ccgcgctgcg ccaacgcttc gccggacaac gccaacagca tggcgatgcc aagcgccacg
     5041 agtttcgtcc aggtctttcg catcgttcgc tcctgttcgg cggctggaag ccgctacggt
     5101 gcggttgtct gttcgatcgc ttgctcgagg gcgccgaggc gctcgcgcac caggtcgttc
     5161 cgccggtcgg ccagccgctg atgccgctcc tgggcgcggc gcaactgggc ctcttgccgc
     5221 ttcaggtcgt aggccgaggt ttcgatctgc acatcgacga gctgctgcac ggcctgcgcc
     5281 agttcggtcc gcagccgctc ggcatcggca ggccggccgg ccagttgcga cgtcagcaag
     5341 tgcgtcagcg tttccagccg ccaggtccgc agcgccaggt cgtgccgctg tgggtcggcc
     5401 tgccgcatcg ccgccagcgt ttcgctgacc cagaagaaat cgcagatcgc ccgttgatat
     5461 tcggacggct ggcgggcctt gagatcctcg agcacgggag gcaactcggg ccggtgcgtc
     5521 gcgacgaacg ccagcgccgc cgcctcgcgc tcgggcgtga acgtgatcga gcggggcgcc
     5581 gtctttggca gccgcatgcc ggccggcggg gcggcttccg catcgcgcgg cgtgggattg
     5641 cgcttgttgg cgggccgagg ctggccgcgc agcccgccgc cagtatcacc gcgctgctgg
     5701 gcgtcggccg cggatacgag ccaggtcaac agcagcacac aaagtgtcgt tcgcgcgatc
     5761 atggattcac tccgcccttg gaggggcgct tggttgcgaa gacccaggag ggaacctccg
     5821 gggctggcgg gggctcctcg ctgtcgtccg gcgactcggg ctggtcgagc cagtcctcca
     5881 aatcagccgc agtccactcc tgttccgact ggaggcgggc ccacacgagg gcatccaccg
     5941 gcgagccgcc cgcaggttgc acgttggtgg cgggactggc attgcggtcg gcggcaggcc
     6001 gccagaacag gttcacggcc agcacggcgc tggccgcgcc gatcgccatc cagcccagcg
     6061 gctgtgccca aaccagcgcg ctttttcgag cgggcgccgc ctggtccgcg ggcggcgccg
     6121 ccgatgccaa tcgctgcgac agtcccaccg cccggctgac ggcgacttgg gccgcctggt
     6181 cgtcggccag gcgtggctcg aaggccgcgg cctctgcggg cgtcagttcg ccggcgacat
     6241 accgaaatgc cagcaggtcg agttcgtcac gttcttcgga caaggctatt cctccaacag
     6301 ctcacccaac gccttcagcg ccaatcgcat ccgcgtcagg accgttccta aagggcagcc
     6361 caactcattg gcgattgctt gaaacgtttg attccggaac attcgccgct cgaccacttc
     6421 ccgctgggcg gcgggcaggg cgtccagcga gctgcgcacg atttcaattt cttcccggcg
     6481 ctcggccgcg tccggcggcg acgcctggct ctgccacacg ggccgggacc acaattcggc
     6541 cagcgccgac tcctgccgga atcgccgccg ccgtgccgcc atggcttcat ggtaggcaac
     6601 cgtgaacagc caggactttg ccgtgtcgag cctcagcgtg gcccagtttt cgaccagctt
     6661 gaggaacacc tgctgcaagg cgtcgtcggc ctcggcccgg ttgcggagca ttcccagcag
     6721 gaactgctgg agcggcagcc gctgctcgcg aaacaactcc gccagggccg cattcgactc
     6781 tgccaattcc gcggacgcat caggctgctg caagcggatg tcccctcgca cgctggacac
     6841 tggtagtact atgcgccaca gtgagcgatt atttcttgga aagcaacgaa tttcatgacc
     6901 tacgacgtca tcattctcgg cacgggcggc gtgggcagcg cggcggcgtt tcatgcggcg
     6961 cggcgggggg ccaaggtgct ggggatcgac cgttttccgg gtgggcacga ccggggcagc
     7021 tcgcacggcg aaacgcggat catccgccag gcctatttcg aacatcccga ctatgtgccg
     7081 ctgctgctgc gggcgtacga gctgtggcgg gagctggagc cgattgcggg cgaaggctta
     7141 cttcacgagg tcggcctgct gcaggtcggc ccgccggagg gcaccgtcgt tcgcggcgtg
     7201 ctggaggcgg cccggctgca tgggctgagc gtcgactcgc tcaacggcga cgaagttcag
     7261 cggcgttact ccggctttcg cgttccccag gactgcgtcg gggtctttga gcccgcggcc
     7321 ggttatctga aggtcgagcg ctgcgtgctg gcgcatttgg atgccgccga gttgcacggc
     7381 gcggagtttc agttcggctc ggcggctgtc tcgcgccaaa ccagcggcag cgacatcgtc
     7441 gtcacgacgg agtccggcaa gtcgttccgg gccaagaagc tgatcgtgac ggccggcgcc
     7501 tgggcaccgc agctgctggc ggaactaaat gtgaagctct tcgtgcgacg aaagcacctg
     7561 tattggtatc cgactgccgc gcccgaatat cgccaggatc gcggctgccc gacgttcctg
     7621 tacgagctgc cgcaaggagt ctattacggc tttcctcaga tcgatacgct cggcgtcaag
     7681 gtcgccgagc acagcggagg agcggtcatc accgacccgc tgaacgatcc gcgcgagctg
     7741 gatgcggcag atctggcccg cgtcgaagcg tttcttgccg cgcaaatgcc cggcgtcacc
     7801 cgccagctcg gccactgctg cgtctgcttc tacacgatgt cgcccgacga gcacttcatc
     7861 gtggaccgga gcccgcgcga cccgcatgta ctatttgccg ccggtttgtc cggtcacggc
     7921 ttcaagttca cgtcggtgct gggcgaagcg ctggcggaac tggcgctcga cggctcgacg
     7981 gacttgccaa tcgatttcct gtcgctggcg cgattcgcga atgacaaatg atcaatccgg
     8041 acaggccaga ggcctatcct acgctggcaa gccagcttga cacttttttc ccagacgcat
     8101 atccttttat aagctgcggt aaagtggcgc aagtccttgc cgcacaacaa tttttcgagc
     8161 taaccagcgc gagctgctcg aaggggtagg tcgcgttttt ttgttgtttg atcg
//
LOCUS       DASRJB010000895         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1118630, whole genome shotgun sequence.
ACCESSION   DASRJB010000895 DASRJB010000000
VERSION     DASRJB010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118630"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFB80_23600"
     CDS             complement(<1..670)
                     /locus_tag="VFB80_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36843.1"
                     /translation="MPCRIRRWTAALTLLLLAFAVPTLAAPPNIVFVIADDLGYGDVG
                     CFGQTKIRTPNIDQLAKDGLRLTQHYSGNAVCAPSRCVLMTGLHPGHAQVRNNRATPP
                     EGQWPLVAGTVTLPRLLQQCGYVTGGFGKWGLGGPGSDGQPLKQGINRWFGYYCQGVA
                     HNHYPTYLWDDDRRFPLKNPEFAAHQKLPDGADVNDPKSYAGYRGTQFAMDLIADEAV
                     KFVRD"
     gene            complement(738..929)
                     /locus_tag="VFB80_23605"
     CDS             complement(738..929)
                     /locus_tag="VFB80_23605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36844.1"
                     /translation="MAELHITLTEPERKCVVEVLERALKETRVEEHRTRAPAYREHIA
                     EQERCLSHVLDKLAPPSHV"
     gene            complement(1106..3079)
                     /locus_tag="VFB80_23610"
     CDS             complement(1106..3079)
                     /locus_tag="VFB80_23610"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HZN36845.1"
                     /translation="MGRRLWWIARWFGGTVFLLLALAYLLLDVFKVGSITLPESQPVQ
                     QVVHLEQGWPQGWKVGETHWFHHANQGTKVLRYDWFLALERPELSVWHVFRTPGLFSS
                     EEYLTRFGFLPSERDQRQNPDQLPVGFAVNDSFQEPFARQPYVPPKEGKPYPYAKPPY
                     RVVGLTCAACHTTQVNYNGTGIRIEGGPGMVDLGTFKDALGRAIFYTNLFPWRFDRFA
                     RRVLGNDYSPAAKAQLRAELKEFLDAAMADQEFAAKKHLHDLKPGFARTDALGLILNR
                     VFKDLNEENLGVTDAPVNFPHIWDATWFDWVQYNASIRPPMVRNIGEALGVGGMVNLD
                     PAKGEMWKSTVDVENLHRMEQQLSGPAPFQGLRAPKWPAEILGAIDSEKQQQGMALYD
                     ARCKSCHWLVDDATAALEGGNQAEISRYWTDPNDFGKRFLKTNFGRSGDLERIGTDAS
                     QALNFARRVVVVVPGQQLMVAGQALDYTTSRVREQKYKELGLTADEKIEYDAFRLPWE
                     TFLDQPLSQRADGTPDQRNNAKLFDDFPAYQDSSARLAYKARPLNGIWATAPFLHNGS
                     VPNLYQLLSPATERDKTFYLGSKEFDPEKVGVVTKPIAGGSLLDTSLPGNLNLGHEFR
                     DLAPGEAKSTKGVLGPALKHDERMALIEYLKSL"
BASE COUNT          542 a         1050 c         1086 g          639 t
ORIGIN      
        1 tgtcccgcac gaacttcacg gcttcgtcgg cgatcaggtc catcgcgaac tgcgtgccgc
       61 gatagccggc ataggacttg gggtcgttga cgtcggcccc gtcgggcaac ttctgatggg
      121 ccgcgaactc agggttcttc agcggaaacc ggcggtcgtc gtcccacagg tacgtcgggt
      181 aatgattgtg cgccacgccc tggcagtagt agccgaacca gcggttgatg ccctgcttca
      241 gcggctgccc gtcgctgccc ggcccgccca ggccccactt gccgaagccg ccggtgacat
      301 agccgcattg ctgcagcagc cgcgggagcg tcacggtgcc ggcgacgagc ggccactgcc
      361 cttcgggggg cgtcgcgcgg ttgttgcgga cttgcgcgtg accgggatgc aggccggtca
      421 tcagaacgca ccgcgatggc gcacagacag cgttccccga gtaatgctgc gtcagccgca
      481 ggccgtcctt ggcgagctgg tcgatattgg gcgtgcggat tttcgtctgg ccgaagcagc
      541 cgacgtcgcc gtagcccagg tcgtcggcga tgacgaacac gatgttgggg ggcgcggcca
      601 atgtcggcac ggcgaaggcc aaaagcagca gcgtcagggc ggccgtccag cggcggatgc
      661 ggcaaggcat aggttgatcc tgcggagagg cagattctga ttacgcgggc cgattgtcgc
      721 ccggcggcgg ccggcggtca aacgtggctg ggcggcgcga gcttgtcgag cacgtgcgac
      781 aggcaccgct cttgctcggc gatgtgctcg cgataggcgg gcgcgcgagt gcggtgctcc
      841 tcgacgcggg tttctttgag cgcccgctcc agcacttcga ccacgcactt gcgttccggt
      901 tcggtgagcg tgatgtgcaa ttcggccatg gctttgccct tccgtaggtc ccgcctgccg
      961 ggcgggacct caggccgcgg gtggcattgg ttcaggtcgc tgcgcttccc ttcaggcccc
     1021 ttccggcaca agggcctact tcttgcagaa tacacctcgc gctcttcggc aagcaaccag
     1081 ttgcctcccg atcgcggcgg ccgtttcaca gcgacttcag atactcgatg agtgccattc
     1141 gctcgtcatg cttcagggcg gggcccaaga cgcccttggt gctcttcgct tcgccaggcg
     1201 ccagatcgcg aaactcgtgc cccaagttca agttgccggg gagcgacgtg tcgagcagag
     1261 agccgccggc aatcggcttc gtcacgacgc cgaccttctc agggtcgaat tccttggagc
     1321 cgagatagaa cgtcttgtcg cgctcggtgg ccggcgagag cagttgatag aggttcggca
     1381 ccgagccgtt gtgcagaaag ggcgccgtgg cccagatgcc gttcagcggc cgggccttgt
     1441 aggccagccg ggcgctggaa tcctggtagg cggggaagtc gtcgaacagc ttggcgttgt
     1501 ttcgctggtc gggagtgccg tccgcgcgct ggctgagcgg ctggtccagg aatgtctccc
     1561 agggcaggcg aaaggcgtcg tattcgatct tttcgtcggc cgtcagcccc agctccttat
     1621 acttctgctc gcggacgcgg ctagtcgtat agtcgagcgc ctggccggcg accatcagct
     1681 gctggccggg cacgaccacg accacgcggc gggcgaagtt caaggcttga gaagcgtcgg
     1741 tcccaatccg ctccaggtcg ccgctgcggc cgaagttggt cttgagaaag cgcttgccga
     1801 aatcgttggg gtcggtccaa tagcggctga tttctgcctg gttgccacct tcgagggcgg
     1861 ccgtcgcgtc gtccacgagc cagtggcagc tcttgcagcg ggcgtcgtag agcgccattc
     1921 cttgctgctg tttctcggag tcgattgctc ccaggatttc cgccggccac tttggcgctc
     1981 tcaggccctg aaacggcgct gggccggaaa gctgctgctc catccgatgg aggttttcga
     2041 catccacggt cgatttccac atctcgccct tggcgggatc gaggttgacc attccgccca
     2101 cgccgagggc ctcgccgatg ttgcggacca tcggcggccg gatcgaggcg ttgtattgca
     2161 cccagtcgaa ccaggtcgcg tcccagatgt gcgggaaatt gaccggggcg tcggtgactc
     2221 ccaggttctc ctcgttgaga tctttgaaga cgcggttgag gatcagcccg agcgcgtcgg
     2281 ttcgagcaaa tccgggcttc aggtcgtgga ggtgcttctt ggcggcgaat tcctgatcgg
     2341 ccatcgccgc gtccaggaac tccttcaatt cggcccgcag ctgcgccttc gctgcgggac
     2401 tgtaatcgtt gcccagcacg cggcgggcaa accggtcgaa acgccagggg aaaaggttcg
     2461 tgtaaaaaat ggcacggcct agcgcgtcct tgaacgttcc cagatcgacc atccccggcc
     2521 cgccttcgat ccggatgcca gtgccgttgt aattgacttg cgtcgtgtgg caggcggcgc
     2581 acgtcagccc gacgacgcga tagggcggct tggcatacgg ataaggtttg ccttccttcg
     2641 gcggcacgta gggctgcctg gcaaacggct cctgaaagct gtcgttcacc gcgaagccga
     2701 ccggcagctg gtcgggattc tgccgctggt cgcgctcgct aggcaggaac ccaaaccgcg
     2761 tcaggtattc ttccgagctg aacagcccgg gcgttcgaaa gacgtgccag accgacagct
     2821 ccggccgctc cagggccagg aaccagtcat accgcagcac tttggttccc tggttggcgt
     2881 gatggaacca gtgggtctcg ccgaccttcc aaccttgtgg ccagccctgc tccaggtgaa
     2941 cgacctgctg aaccggctga ctctcgggca gggtgatgct gccgactttg aacacgtcca
     3001 gcagcagata cgccagggcg agcagtagga agacagttcc gccgaaccac ctggcgatcc
     3061 accaaaggcg tcttcccatg gtgcctgcct ccacgcccgc gcaatgcgat tcgcaatccc
     3121 ctctctatag tcgcaatgcg gagcaatcgg cgggcatgta caatcggggc cgctattcgg
     3181 atggcttctg tcggatctct gcggaacctc tcatgactcg gcattaccgc ttgcgcaccg
     3241 catggcaaac catcgccatc atcctcgttg ggttccaggc tctgccagcg ccggcccagg
     3301 aagcaaagca gccgccg
//
LOCUS       DASRJB010000896         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2524849, whole genome shotgun sequence.
ACCESSION   DASRJB010000896 DASRJB010000000
VERSION     DASRJB010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524849"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFB80_23615"
     CDS             <1..857
                     /locus_tag="VFB80_23615"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36846.1"
                     /translation="ALHNYHDTFQRFPPAGLNYGWCQSPPPATNTSNNRILNVNGLLL
                     MLPFYEQTAAAQQFVNTAAASNVTVGNDGCCPPTAAQEQLAGGGVPAVNAQIARMHLK
                     IFLCPSDITDPSIPNVTDIYSIGYNLGNLGRGARTNYDFSSLLAFRCDEWTRQRNNNT
                     RRMFAQNSDCRFADVTDGTSNTVAFCESTRDTNNGETTTWAYRGWVMIGNDIGTPNGI
                     NNWIYPTNPILKVGRVGSWGWPGSLHPNGCMVGMGDGAVRFVPQNAAQVVRDAMARIS
                     DGNTFDLP"
     gene            887..1339
                     /locus_tag="VFB80_23620"
     CDS             887..1339
                     /locus_tag="VFB80_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36847.1"
                     /translation="MQRYLIAAALVALAIAGCGGGSAAPPVEGVVTLDGKPLANATVQ
                     LIPQGQNMGQTGFGRTSADGKFTIASADGRATGALAGEYKVVISKHIRPDGSDYIAKP
                     DEDPMLAAYKELLPPVYSNAEQTKLQTTIGAEGKKDLNFNLTKNGRGS"
     gene            1377..2498
                     /locus_tag="VFB80_23625"
     CDS             1377..2498
                     /locus_tag="VFB80_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN36848.1"
                     /translation="MPKYRVAVIGHTGHGNYGHGIDTVWKQLPEQCQVVAVADRDEQG
                     RADAIKRLTTADSKTPPKGYADYRQMLDAEKPQIVAIGPRWLGEHRDMVLAAAERGIH
                     IYMEKPMCRTLAEADEMVAACEAKDVKLALAHQTRYSPRLQAIRDLIDGGQLGTVLEL
                     RGRGKEDPRGGGEDLWVLGSHIMNLIHYFGGRPNWCFARVEQDGQPVTKAAVKDGPEA
                     IGPLAGDNVAAMYGLENGATAYFGSRRGMGGGAGRFALQIFGSKGIVEIVTGHLPSVQ
                     FLADPAWSPGRSKAQWQPVTSAGVGKPEPLKEGGLDAGNVLAVKDLLSAIENDRQPEC
                     SVLEGRTTVEMIAAVFESHRVGGPVTFPLKTRVNPLSLL"
     gene            complement(2507..3136)
                     /locus_tag="VFB80_23630"
     CDS             complement(2507..3136)
                     /locus_tag="VFB80_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36849.1"
                     /translation="MTSSQKTLIAGCLIGLVGSGVAVGFARYQQQSLDFYGSQPQAVS
                     VATLIEQGHHGPRWIELTNWQLGPRAVVSDEHGRIEAIRLPVFPRGQTPTPIHVVLRS
                     TKSRNLDDFSLRLVSRETFRGALLDAGPGDGLRKQLAAAYPGQPLADQICELDIDFGG
                     PPFARWSSTVQSASAGLLLFGIICMLGFIGSSFARKDDLPVLESVPSGR"
BASE COUNT          633 a         1131 c         1074 g          488 t
ORIGIN      
        1 tggccctgca caactatcac gacacctttc agcggtttcc gccggcgggc ttgaactacg
       61 gctggtgcca gtcgccaccg cccgccacca acacgtcgaa caatcgcatt ctgaatgtga
      121 acggattgct cctgatgctc cccttttacg agcagactgc cgccgcccaa caattcgtca
      181 ataccgcagc cgccagcaac gtcaccgtgg gcaacgatgg ctgctgtcct ccgactgcgg
      241 cccaggagca gctcgccggc ggcggcgtgc cggccgtcaa tgcccagatc gcccgcatgc
      301 acctcaaaat cttcctctgc cccagcgaca tcaccgatcc ttccatcccg aacgtcacgg
      361 acatttactc gatcggctac aaccttggca acttgggccg cggagcacgg acgaactacg
      421 acttctcctc gctgcttgcc tttcgctgcg acgagtggac gcggcagcgg aacaacaaca
      481 cgaggcggat gttcgcccag aacagcgact gccgctttgc cgacgtgacc gacggcacga
      541 gcaacacggt cgccttctgc gaatcgacgc gcgacacgaa caacggcgag acgaccacct
      601 gggcctatcg cggctgggtc atgatcggca acgacatcgg cacgcccaac ggaatcaaca
      661 actggattta tccgacgaat ccgatcctca aagtcggccg cgtcggcagc tggggctggc
      721 ccggcagcct gcatcccaac ggttgcatgg ttggcatggg ggacggcgcg gtgcggttcg
      781 tgccgcagaa cgccgcccag gtggtccgcg acgccatggc ccggatttcc gacggaaaca
      841 cgttcgacct gccttagcga agcccgccgc cagtaactta gcgaagatgc agcgatattt
      901 gattgcggct gcgctcgtcg ccctggccat cgccggctgc ggtggcggga gcgcggcccc
      961 gcccgtcgag ggcgttgtga cgctggatgg aaaaccgctg gccaacgcca ccgtgcaact
     1021 gattccgcag ggccagaaca tgggccagac cggcttcggc cgcaccagtg ccgacggcaa
     1081 atttacgatc gcctcggccg acggcagggc gacgggcgct ctggccggtg agtataaggt
     1141 ggtcatcagc aagcacattc gccccgacgg cagcgactat attgccaagc cggacgaaga
     1201 cccgatgctg gcggcctaca aagagctgct cccgccggtc tacagcaacg ccgagcaaac
     1261 gaagctccaa accaccatcg gcgctgaagg caagaaggac ctgaacttca acctcaccaa
     1321 aaacggccgc ggctcctaac tgccgatccc cgactcccga cccccggagc tctcccatgc
     1381 ccaaatatcg cgtcgcagtc atcggtcaca cgggccacgg aaactatggc cacggcatcg
     1441 acacggtctg gaagcagctt ccggagcaat gccaggtggt cgcggtggcg gatcgtgacg
     1501 agcagggccg cgcggatgcc ataaagcgtc tgaccaccgc cgacagcaag acgccgccca
     1561 aaggctatgc cgattaccgg cagatgcttg acgccgagaa gccgcagatc gttgccatcg
     1621 gcccgcggtg gctgggcgag catcgcgaca tggtgctggc ggccgccgag cgcggcatcc
     1681 atatctacat ggaaaagccg atgtgccgca cgctcgccga ggccgacgaa atggtcgccg
     1741 cctgcgaggc caaggacgtg aagctggccc tggcccatca gacccgctac agcccgcggc
     1801 tgcaggcgat ccgcgacctg atcgacggcg ggcagctggg cacggttttg gagctgcgcg
     1861 gccgcggcaa ggaagacccc cgcggcggcg gcgaggacct gtgggtgctc ggcagccaca
     1921 tcatgaacct gatccactat ttcggcggcc gacccaactg gtgctttgcc cgcgtcgaac
     1981 aggacggcca gccagtgacc aaagcggccg tgaaggacgg ccccgaggcc atcggcccgc
     2041 tggctggcga caacgtcgcc gcgatgtacg gcctggagaa tggcgcgacg gcctatttcg
     2101 gctcgcgacg gggaatgggc ggcggcgcgg gccgctttgc cttgcaaatc ttcggcagca
     2161 aggggatcgt cgaaatcgtc accggccatc tgcccagcgt gcagttcctg gccgatcccg
     2221 cctggtcgcc cggccgcagc aaggcccagt ggcagccggt caccagcgcc ggcgtcggca
     2281 agccggagcc gctcaaggag ggcggcctcg acgccggcaa cgtgctggcg gtgaaggacc
     2341 tgctctcggc gatcgagaac gaccggcagc cggagtgcag cgtgctggaa ggccggacga
     2401 ccgtcgaaat gatcgccgcg gtgttcgagt cgcaccgcgt cggcggccca gtcacattcc
     2461 cgctcaagac gcgcgtcaat ccgctctcgc tgttgtaacc aagcgactac cgcccgctgg
     2521 gcacggactc cagcacgggc aggtcgtcct tgcgggcgaa gctgctgccg atgaagccca
     2581 gcatgcagat aattccaaac aacagcagcc cggcggacgc ggactggacg gtgctcgacc
     2641 agcgggcaaa gggcggcccg ccgaaatcga tgtcgagctc gcagatctga tcggccagcg
     2701 gctggcccgg ataggccgcg gccagttgct tgcggagccc gtcgccgggc cctgcatcga
     2761 gcagcgcgcc gcggaaggtc tcgcgcgaaa cgagccgcag gctgaagtcg tccaggttgc
     2821 ggctcttggt gctgcgcagc acgacgtgga tcggcgttgg cgtctggccg cgcggaaaga
     2881 cgggaaggcg gatcgcctcg atccgtccgt gctcgtcgct gacgaccgct cgcggtccga
     2941 gctgccagtt cgtcagctcg atccaccgcg ggccgtggtg gccctgctcg atcagcgtgg
     3001 ccacgctcac cgcctgcggc tgcgagccgt agaagtccag cgattgctgc tgatagcggg
     3061 cgaagccgac ggccacgccc gaacccacga gtccgatcag gcaaccagcg atcaacgttt
     3121 tttgggagga ggtcatcgcc cacctcgagg caatagcggc ccggcggcga aacaccgccg
     3181 ctcgtcgcag caaccctagg tcagggctta cgcgaagcaa tccagcccgc gggccggttg
     3241 cgccgatcgc agcaggtttg cgggcgctac gccaccatcg cgcgggggcg ggtcagcagg
     3301 ctcaggaggg ctgagaacag cgctgc
//
LOCUS       DASRJB010000897         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1394647, whole genome shotgun sequence.
ACCESSION   DASRJB010000897 DASRJB010000000
VERSION     DASRJB010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394647"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFB80_23635"
     CDS             complement(<1..595)
                     /locus_tag="VFB80_23635"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZN36850.1"
                     /translation="MTTPDFAAQRRAMVEHQLRRRGIKDERVLEAMARVPREAFLTPD
                     LAGEAYSDCALPIDCDQTISQPVIVAMMTEALHLSGRERVLELGTGSGYQTAILAELA
                     AEVVSIERHAELSQQAGERLRTLGFQNVRLRVGDGSLGWPEAAPFDRIIITAAAEEPP
                     PAVWEQLTEGGVLVGPFGTAAEQLLYEMHKIGGQTKSQ"
     gene            complement(592..1848)
                     /locus_tag="VFB80_23640"
     CDS             complement(592..1848)
                     /locus_tag="VFB80_23640"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN36851.1"
                     /translation="MNDSEQFLRQHQRLTRRFFFRAGAAGAAALGFAPVLLAAERAPE
                     LTAAIDKLEPFFTPPANFRDVSRGKPLPHSLPEEKAREVGLTRETWKLEVISDPENPA
                     KLGRQLTKADNTALDFAGLLKLGEKHAVRFAKVMTCLNIGCPLGMGIWEGVPLREVLW
                     LTQPREDVRRVFYYGYHNDDEKQMFQSSLPLGRVLEDYHDLPPVILCYKLNGQWLSPQ
                     RGGPVRIVVPETYGYKSIKWLSHVVLTNLPHANDTYANGNNDLDSPLKNFSAVLSVPK
                     EMTANTPIPVTGYAQVGISGVSKVQVWINPSNVDWPADDKYFATAPWTDAEILGPPRQ
                     WGGELPDGNIPAKTVGFDEAGRPLRWPLPLGKLHWAALWPGLPTGDYTLRSRTIDERG
                     IAQPLPRPFRKSGHAGIESVNIKVRS"
     gene            2089..3294
                     /locus_tag="VFB80_23645"
     CDS             2089..3294
                     /locus_tag="VFB80_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-directed GTPase
                     activating protein isoform b"
                     /protein_id="HZN36852.1"
                     /translation="MTRTVYLVATLSAGVLLAGCNQAGDANSAATTAAMPVSSGNAAA
                     DPADLPSDAELLKQIDEALEYTFDHRRLSVGQGANDQAAWQIIHGALAFKREFLVRDG
                     DKDVSAVDYILGGGSMKGLSLRRGDPLDDEMKPSIPGGGLRYGITTVVANDKMGQGHT
                     DQWLGYLSDCKLPLEQPLVVEGRPHTIADYIDQAKRDVHKNPIREYSWTLMALNSYYP
                     TDYTWQAADGSEWSIAKLVEIELEHSLDQSPCGGTHRMTALTMAYNRRQVEGKPFEGV
                     WKSLAERIEQCVNKAREFQNPDGSLSSNYFARPGKSADLAICMGSAGHVMEFIATALD
                     KDQLQEPWVKRAVLDVCKLFRKTKPVDVECGALFHAAHGLVLYREKVFGPRTFGKEAA
                     AAVTAAKPE"
     gene            3358..4617
                     /locus_tag="VFB80_23650"
     CDS             3358..4617
                     /locus_tag="VFB80_23650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN36853.1"
                     /translation="MSAPRNMGFLLTLRIAIRALRKNKLRAGLTVLGVVIGIAAVTAM
                     VSIGQSASELVKGQFESIGTNVIVVFPGNQRQGGVRQGLVKTLTAADSDAIARECPSI
                     LASTPLVGAQAHVIYGNTNYSPRDVFGVGTDYPIVGSWVMASGDFFTERDVTASAKVC
                     VIGRTLVAKLFQTSDPINQTIRVKNIPFRVVGVLQAKGANMVGDDQDDILLMPYTTVR
                     NRLQGSEFDNVNAVICISRSLALLPDAQHEITQLLAERHHIHPGEPNDFIVRNVAEIA
                     NVFGIVVGTLTMMIVSIAAISLLVGGVGIMNIMLVSVTERTREIGIRMAVGARGRDIL
                     FQFLVEAILLSCIGGAIGFALGCGASVGLTLLINSLTSGTKWPIVISFNAAVLSFVFA
                     AAVGVFFGFYPARRASQLDPIEALRYE"
     gene            4614..>4906
                     /locus_tag="VFB80_23655"
     CDS             4614..>4906
                     /locus_tag="VFB80_23655"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZN36854.1"
                     /translation="MKILELYCGIGGLAEAVGDAATIAAAVDINRAALDVYSSNFSHP
                     AHCRTIESLPAEFYESAAAELWWFSPPCQPFTRRGRKRDLDDPRCASLVSVVRR"
BASE COUNT          821 a         1617 c         1602 g          866 t
ORIGIN      
        1 tttgcgattt ggtctggccg ccgatcttgt gcatttcgta gagcagttgc tcggcggcgg
       61 tgccgaaggg gccgacgagg accccgccct cggtgagctg ctcccagacg gccggcggcg
      121 gctcttcggc cgccgcggta atgatgatcc ggtcgaatgg cgcagcttcc ggccagccga
      181 gcgagccgtc gccgacgcgg agccgcacgt tctgaaagcc cagcgtccgc agccgctcgc
      241 cggcttgctg cgatagttcg gcatgccgtt cgatcgaaac gacctcggcg gccaattcgg
      301 ccaggatcgc cgtctgatag ccgctgccgg tgcccagttc gaggacgcgc tcgcggcccg
      361 acaggtgtag cgcctcggtc atcatggcca cgatcaccgg ctggctgatc gtctggtcgc
      421 agtcgatcgg tagggcgcaa tcggagtagg cttcgccggc gaggtccggc gtcaagaatg
      481 cctcgcgggg aacgcgcgcc atcgcctcca gcacgcgctc gtccttgatc cctcgtcggc
      541 gcaattggtg ctcgaccatc gcgcggcgct gggcggcaaa gtcgggcgta gtcacgatcg
      601 gaccttgatg ttgacgcttt caattcccgc gtggcccgat ttgcgaaagg gccgcggcag
      661 cggctgggcg atgcctcgct cgtcgatcgt ccgcgagcgg agcgtgtagt cgccggtagg
      721 caatccgggc cagagcgccg cccagtgcaa tttccctaga ggcagcggcc agcgcagcgg
      781 ccgtcccgcc tcgtcgaagc caaccgtttt cgcggggatg tttccgtccg gcaattcacc
      841 gccccactgc cgcggcgggc cgagaatctc ggcgtcggtc cacggcgcgg tggcaaagta
      901 cttatcgtcc gccggccaat ccacgttgct gggattgatc cagacttgca ccttcgatac
      961 gccggagatt ccgacctggg cgtagccggt gaccgggatc ggcgtgttgg cggtcatctc
     1021 cttcggcacc gagagcaccg cggaaaagtt cttcagcggg ctgtcgaggt cgttgttgcc
     1081 attggcgtac gtgtcgttgg cgtgcggcag gttcgtcagc acgacgtgcg acagccactt
     1141 gatcgacttg tagccgtagg tctccggcac gacgattcgc accggcccgc cgcgctgcgg
     1201 gctgagccac tggccgttga gcttgtagca caagatcacc ggcggcaggt cgtgatagtc
     1261 ctccagcacg cggcccaagg gcagcgagct ctggaacatc tgcttctcgt cgtcgttgtg
     1321 gtagccgtag tagaagacgc ggcggacgtc ctcccgcggc tgcgtgagcc acaagacttc
     1381 gcgaagcggc acgccctccc agattcccat gcccagcggg cagccgatgt tgaggcaggt
     1441 catcaccttg gcaaaccgca cggcgtgctt ctcgcccagc ttcagcaggc cggcaaaatc
     1501 gagagcggtg ttgtcggcct tcgtcaattg ccggccgagc ttcgccgggt tttccggatc
     1561 gctgatcacc tccagcttcc aggtctcgcg cgtcaggccg acttcgcgag ccttttcttc
     1621 cggcagcgag tgcggcagcg gcttgccccg cgagacgtcg cgaaagttgg ccggcggagt
     1681 aaagaacggc tccagcttgt cgatcgccgc agtcaactcc ggtgctcgtt cagcggccag
     1741 cagcaccggc gcgaagccca gcgccgccgc tcccgccgcc ccggcccgga agaagaaccg
     1801 gcgcgtcagc cgctggtgct gccggaggaa ttgctcgctg tcgttcatgg ccgtatctcc
     1861 cggtgccctg atgatagccc tggcacctgc gagggggaaa caacttccaa ctcctgggac
     1921 gatcggcgat acagtttccg cagtttgtgc agctcacgca gattaccgca gttgtggctg
     1981 ctcccttgtc cttggggtat atttgcaagc tgcaccggtc cgcggtacga ggccgccggc
     2041 catccctccc tccgcagtcc cgcagcggct gcgtctggat ttctgcgtat gactcgcacc
     2101 gtgtacttag tggccactct atcggccggc gtcctgctcg ccggctgcaa ccaggcgggc
     2161 gatgccaatt cggccgcgac aaccgcggcg atgcctgttt ccagcggcaa tgccgcggcc
     2221 gatccggccg acctgcccag cgacgccgag ctgctgaagc agatcgacga agccttggaa
     2281 tacacgttcg atcatcgccg gttgtcggtc ggccaagggg cgaatgacca ggcggcatgg
     2341 cagatcatcc acggggcgct ggccttcaag cgcgaattcc tggtccgcga cggcgacaaa
     2401 gacgtctcgg ccgtcgatta catcctcggc ggcggcagca tgaaggggct ttcgctgcgg
     2461 cggggcgatc cgctggatga cgagatgaag cccagtattc ctggcggcgg gctgcgctat
     2521 ggcatcacca ccgtcgtcgc caacgacaag atgggccagg ggcataccga ccagtggctg
     2581 gggtacctct cggattgcaa gctgccgctg gagcagccgc tggtggtcga gggccggccg
     2641 cacacgatcg ccgactacat cgaccaggcc aagcgggacg tccacaagaa cccgattcgc
     2701 gaatacagct ggacgctgat ggcgctgaat tcgtattacc cgaccgatta cacctggcag
     2761 gcggccgacg gcagcgagtg gagcatcgcg aagctggtcg agatcgagct ggagcacagc
     2821 ctcgaccaga gtccctgcgg cggcacccac cgaatgaccg cgctgacgat ggcctataac
     2881 cggcggcagg tggaaggcaa gccgttcgag ggtgtctgga agagcctcgc cgagcggatc
     2941 gagcagtgtg tgaataaggc ccgcgaattt cagaaccccg acggctcgct gtcgtcgaac
     3001 tatttcgccc gccccggcaa atcggcggac ctggcgatct gcatgggctc ggcgggccac
     3061 gtgatggaat tcatcgccac ggccctcgac aaggaccagc tgcaagagcc gtgggtcaag
     3121 cgggcggtgc tcgacgtttg caagctcttt cgcaagacga agccggtcga tgtcgagtgc
     3181 ggggcgctgt tccacgccgc ccacggcctg gtgctctatc gcgagaaggt tttcgggccg
     3241 cggacgttcg gcaaagaagc cgcggcagcg gtcacggcag cgaagccgga gtaagatacg
     3301 tggggcagac attccggtct gccttacatc tgcgtcgcaa attcgtggca gacaagaatg
     3361 tctgccccac gaaacatggg cttcctgcta accctccgca tcgccattcg cgcattgcgg
     3421 aagaacaagc tccgcgcggg gctgacggtg ctgggcgtgg tgattggcat cgcggccgtc
     3481 acggcgatgg tctcgatcgg gcaaagcgcc agcgaactcg tgaaagggca gttcgagtcg
     3541 atcggcacca acgtgatcgt ggtcttcccc ggcaatcagc ggcagggggg cgtgcggcag
     3601 gggctggtca agacgctgac cgcggccgac tcggacgcga tcgcccgcga atgcccctcg
     3661 atcctggcca gcacgccgct ggtcggcgcg caggcccacg tcatctacgg caacacaaac
     3721 tacagccccc gcgacgtgtt cggcgtcggg accgactacc caatcgtcgg cagctgggta
     3781 atggccagcg gcgacttctt caccgagcgc gacgtcaccg cctcggccaa ggtctgcgta
     3841 atcggccgca cgctcgtggc caaactcttt cagaccagcg acccgatcaa ccagacgatc
     3901 cgcgtgaaga acatcccgtt ccgcgtcgtc ggcgtgctgc aggccaaggg ggccaacatg
     3961 gtcggcgacg accaggacga catcctgctg atgccctaca caaccgtccg caaccgcctg
     4021 cagggctcgg agttcgacaa cgtcaacgcc gtcatctgca tctcgcgctc gctggccctg
     4081 ctgcccgacg cccagcacga gatcacgcaa ctgctggccg agcggcacca cattcacccc
     4141 ggcgagccga acgacttcat cgtccgcaac gtcgccgaaa tcgccaacgt cttcggcatc
     4201 gtggtcggca cgctgacgat gatgatcgtc tcgatcgccg ccatctcgct gctcgtcggc
     4261 ggggtgggaa tcatgaacat catgctggtc agcgtgaccg agcggacccg cgagatcggc
     4321 atccgcatgg cggtcggggc ccgcggccgc gacatcctgt ttcagttcct cgtcgaggcg
     4381 atcctgctct cctgcatcgg cggcgcaatc ggcttcgcgc tcggctgcgg ggcctccgtc
     4441 ggcctgacgc tgctcatcaa ctcgctgacc agcgggacca agtggccgat cgtcatttcg
     4501 ttcaacgccg cggtcctgtc gttcgtgttt gcggccgcgg tcggcgtgtt tttcggcttc
     4561 tacccggcgc ggcgggcgag ccagctcgac ccgatcgaag ccctccgcta cgaatgaaga
     4621 ttctcgagct gtactgcggc atcggcgggc tggccgaggc ggtcggcgat gcggcaacaa
     4681 tcgcggccgc cgtcgacatt aaccgcgcgg cgctggacgt gtattcgagc aacttctcgc
     4741 atccggcgca ctgccgcacg atcgaatcgc tgcccgcgga gttttatgaa tcggccgcgg
     4801 cagagttgtg gtggttctcg ccgccttgcc agccgttcac gcgccgcggc cggaagcgcg
     4861 acctggacga tccgcggtgc gcgtcgctgg ttagcgtcgt gcggcg
//
LOCUS       DASRJB010000898         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2237286, whole genome shotgun sequence.
ACCESSION   DASRJB010000898 DASRJB010000000
VERSION     DASRJB010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237286"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VFB80_23660"
     CDS             <1..1521
                     /locus_tag="VFB80_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36855.1"
                     /translation="SRDWHFDIQHALAQVRRLREMSPGENVILAVVQAPQLSWPAFRT
                     AEKESGEIIAGLVDSLAGETEAKRIVLTGHSGGGSFTFGCLDAWDAIPNSIERIVFLD
                     SNYAYADDKRHADKLLAWLRGQRSRRLVVIAYDDREITLSGKKVVSPTGGTWRASQRM
                     LDRFTKDVELPEVKNGPFLHRRGLNGQIEFFLHPNPENKILHTALVGEMNGLVHALTL
                     GSGPENRWGTFGGPRAYGKWVQQNAPTDYTLARARDSDVRLQLPARAAAAPTGSAFAE
                     QIESLPREEREATILKEISSGNVPDFLRVLTAVEVAAVDDRGERHTATYFAMPDYLAV
                     GTDDDFFRLPMSPITAQKFAASAGASLITTKISDDLWQAANLKLEPKPLTKDRDAAAT
                     FFQHHQIIEQQRKGRPHSRLIAGIKKDVVLTNRLQEKPGRVAIYGWHYASGRPIQPLY
                     VGHIDWYVDYSHGIRLVAQRLIVDGKETTVSEMLADPVFHVLLSNEGPITAGYETK"
     gene            1518..2225
                     /locus_tag="VFB80_23665"
     CDS             1518..2225
                     /locus_tag="VFB80_23665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN36856.1"
                     /translation="MTRQVLVTGGTSGIGWAIADAFAREGCRVLATGLTNAEAARTPR
                     IAALEARQLDVTQPAEIAAVIAELKSLDVLVNAAGTIVRNGGEHDPEVFARVIDVNLN
                     GAMRVCAAARPLLVKTRGCVINIASMLSFFGSGYAPAYSASKGGVAQLTKSLAIAWAA
                     EGIRVNAIAPGWIETALTAPLVADLARRDAIVARTPLGRWGQPADVAGAALFLASPAA
                     AFITGVILPVDGGYSVA"
     gene            2222..2716
                     /locus_tag="VFB80_23670"
     CDS             2222..2716
                     /locus_tag="VFB80_23670"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HZN36857.1"
                     /translation="MNPPPLTSASLMIPEIAIAAVPDDERLWVPQAPSVWFRPLLLNT
                     VNGEWVNLLRVKRAGMLSRHRHPAPVHGYVIRGSWRYLEHDWVAREGMYVFEPPGEIH
                     TLVVDPDVPEMITLFHVCGAVVYYDDQLRPTSHDDVHTKLDLARRHYEQVGLGADYVR
                     QFVR"
     gene            3153..>4810
                     /gene="aspS"
                     /locus_tag="VFB80_23675"
     CDS             3153..>4810
                     /gene="aspS"
                     /locus_tag="VFB80_23675"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZN36858.1"
                     /translation="MLRSHTCGQLRAANVGQEVTLCGWVDTSRDHGGTLFIDVRDRYG
                     KTQAVFAPEAGQELLDESRKVRTEDVIRVTGKVAPRPEGTKNPKLDTGEIELRVKKLE
                     LLNKCHNAPFLPTAMELPGEDLRLTYRYLDLRRPEMQRTLKLRNDIILEMRNYFGEHD
                     FVDVETPILGRSTPEGARDYLVPSRVHNGLFYALPQSPQLYKQILMMAGYDRYVQVAR
                     CFRDEDLRADRQPEFTQLDMEMSFVTNDDVMGVIDGLMQRLAKKFLNLDLKLPLSRMT
                     WDESMERFGHDAPDLRFGCELIDCTDIAATVDFRVFKEIAQAGKKVRGIRAPGGASFY
                     SRKLIDELTEFVKQFEAKGLAWFRVEPDGKLFSPIAKNFTEEQLGKIKERMRAAPGDI
                     LFFVADTWAVSCKALHNLRKRVGVDLKLYDPKAMNFSWIVEFPMFEFDKEENRWASMH
                     HPFTAPRPQDVPMLDSDPGQARAVAYDLVINGSEAGGGTIRIHDQATQSKVFGLLGID
                     AETAKNRFGFLLDALQMGAPPHGGIALGIDRIVMLFGTLENIREV"
BASE COUNT          896 a         1571 c         1538 g          805 t
ORIGIN      
        1 agccgcgact ggcatttcga cattcagcat gccttggcgc aggttcgacg gctgcgggaa
       61 atgtcgccgg gcgaaaatgt gatcctggcg gtcgttcaag cgccgcagct cagctggccg
      121 gcattccgca ccgccgagaa agagtccggc gaaatcatcg caggccttgt cgattcgctg
      181 gctggcgaga cggaggcgaa gcggatcgtg ctcaccggcc acagcggcgg cggatccttt
      241 acgttcggct gcctcgatgc ttgggacgcc attcccaata gcatcgagcg gatcgtgttt
      301 ctcgattcaa actacgccta tgccgacgac aagcggcacg ccgacaagct gctggcctgg
      361 ctgcgcggcc agcgctcgcg gcggctggtc gtcatcgcct acgacgaccg cgaaatcacc
      421 ctcagcggaa agaaagtggt cagcccgacc ggcggaacgt ggcgggcttc gcagcggatg
      481 ctcgatcgtt tcacgaaaga tgtggagctg cccgaagtaa aaaacggtcc ctttcttcac
      541 cggcgaggcc tgaacggcca gatcgagttc ttcctgcatc cgaatccgga gaacaagatt
      601 ctgcacacgg ccctggtcgg cgagatgaac ggcctggtgc acgcactgac gctcgggagc
      661 ggcccggaaa accgatgggg gaccttcggc ggcccccggg cgtatgggaa gtgggtccag
      721 cagaacgcgc cgaccgacta cacactcgcc agagcccgcg actcggatgt gcggctccag
      781 cttccggcgc gggccgctgc tgcaccgacc ggttcagcat ttgcagagca aatcgagtcg
      841 ctgcctcgcg aagagcgcga ggcgacgatt ctcaaggaga tcagcagcgg caatgtgccg
      901 gacttcctgc gggtgctgac ggccgtggaa gtcgccgctg tcgatgaccg cggcgagcgg
      961 catacggcca cgtacttcgc gatgcccgac tacctggccg tcggaaccga cgacgacttc
     1021 ttccgcctgc cgatgtcgcc gatcactgcc cagaagttcg cagcttccgc gggcgcctcg
     1081 ctgatcacga ccaaaatctc cgacgatttg tggcaggcag cgaatctcaa gctggaaccc
     1141 aagccgctga ccaaagaccg cgacgcagcc gcgaccttct ttcagcacca ccagatcatc
     1201 gagcagcagc gcaagggccg gccgcacagc cgacttatcg ccggaatcaa gaaagacgtc
     1261 gtgctaacca accgcctcca ggaaaaacct ggtcgggtcg cgatctacgg ctggcactac
     1321 gccagcggcc gcccgattca gccgctctat gtcggccata tcgattggta cgtcgattac
     1381 agccacggca tccggctggt tgcacagcgc ctgatcgtcg atggcaagga gacgaccgtg
     1441 agcgagatgc tggccgatcc cgtattccac gtcttgctat ccaacgaagg gccaattacc
     1501 gcggggtacg aaaccaaatg acgcggcagg tgctcgtaac cggcgggacc agcggcattg
     1561 ggtgggcgat tgccgacgcg tttgcccgcg agggctgccg cgtcctggcc acggggctga
     1621 cgaatgccga agcggcgcgc acgccgagaa tcgcggcgct ggaggccagg caactcgacg
     1681 tgacgcagcc ggcggaaatc gccgccgtca tcgcggagct gaaatcgctc gacgtgctcg
     1741 tcaatgccgc gggcaccatt gtgcgcaacg gcggcgagca cgacccggaa gttttcgccc
     1801 gcgtcatcga cgtcaatttg aacggcgcca tgcgggtctg cgccgcggcg cggccgttgc
     1861 tagtgaagac tcgcggctgc gtcatcaaca tcgcctcgat gctcagcttc ttcggcagcg
     1921 gctatgcccc cgcctacagc gccagcaagg ggggcgtcgc gcagctcacg aagtcgctgg
     1981 ctatcgcctg ggcggcggaa ggcatccgcg tcaacgcgat cgccccgggc tggatcgaaa
     2041 cggcgctaac cgcgccgctc gtggccgatc tagcgcggcg ggacgccatc gtggcccgca
     2101 cgccgctggg ccgctggggg cagccggccg acgtcgccgg cgcggccctc ttcctggcgt
     2161 cacccgccgc cgcctttatt accggtgtca ttctgcccgt cgatggagga tactcggtcg
     2221 catgaatccg ccgccgctga cgtccgcctc gctgatgatc cccgaaatcg ccatcgcagc
     2281 cgtgccggac gacgagcggc tgtgggtacc gcaagcgccg agcgtctggt tcaggccact
     2341 gctcttgaac acggtcaacg gcgagtgggt gaatttgctg cgcgtgaagc gggccgggat
     2401 gctcagccgg catcgtcatc ccgcgcccgt ccacggctac gtgatccgcg gctcctggcg
     2461 gtacctggag cacgactggg tcgcccgcga agggatgtac gtcttcgagc cgccggggga
     2521 aatccacacg ctggtcgtcg atcccgacgt gccggagatg atcacgctgt tccacgtttg
     2581 cggggccgtc gtctattacg acgaccagct gcggcccact agccacgacg acgtacacac
     2641 caagctcgac ctggcccgcc ggcactacga gcaggtcggc ctcggcgccg actacgtgcg
     2701 gcaattcgtg cggtagcgat tgtcaacagc ttgatttgga ttggattgac atacgtgaac
     2761 aagtttgtac aattcgcgcg gcgattgccg ggccaggtag cgaaagtccg gcatcgagcg
     2821 atacgtggca ggactcctcc aattggggac tgctcagcga tttgcagcct cgacggttgc
     2881 cggtcgcgta acctgctggc atgtaacgac ttgcgacgcg cagcttgcag ggttggcagt
     2941 tttcaatcgc ctgctcaccc ctgaatcgcc acggaggaaa ctacgctccc ttggagcagg
     3001 aaccgtcgcg ctctcgccgc acgagcaggc tgcgagctga acctatgcgc tccaaggtgg
     3061 tctgatacaa tgccccgttt atcagcggct tcgcggcgat gcgcctctcg cgtaaatcgc
     3121 ggccgcggca cgctttacgt cggagtcttg ccttgcttcg ctcccatacc tgcggccagc
     3181 tcagggccgc gaacgtcgga caagaggtca cgctctgcgg ctgggtcgac accagccgcg
     3241 atcacggcgg cacgctgttc atcgacgtcc gcgaccgcta cggcaagacg caggcggtat
     3301 tcgcccccga ggccgggcag gaattgctcg acgaatcgcg gaaagtgcgg accgaggacg
     3361 tgatccgcgt cacgggcaag gtcgccccgc ggccggaggg cacgaaaaac cccaagctcg
     3421 acaccggcga gatcgagctc cgcgtcaaaa agctcgagct gctcaacaag tgccacaacg
     3481 cccccttcct gccgacggcg atggagttgc cgggcgagga cctgcggctg acgtaccgct
     3541 atctcgatct gcggcggccc gagatgcagc ggacgctcaa actccgcaac gacatcattc
     3601 tggagatgcg gaattacttt ggggagcacg acttcgtcga cgttgaaacg ccgatcctcg
     3661 gccgcagcac gcccgaaggc gcccgcgact acctggtgcc cagccgtgtg cacaacggcc
     3721 tgttctacgc cctgccgcag tcgccgcagc tctacaagca aatcctgatg atggccggct
     3781 acgaccgcta cgtgcaggtc gcccgctgct tccgcgacga agacctgcgg gccgaccggc
     3841 agccggagtt cacccagctc gacatggaga tgtcgttcgt caccaacgac gacgtgatgg
     3901 gcgtgatcga cgggctgatg cagcggctgg cgaagaagtt cctcaatctc gatctgaagc
     3961 tgccgctgtc gcggatgacc tgggacgagt cgatggagcg ttttggccac gacgcgcccg
     4021 acctgcgttt tggctgcgag ctgatcgact gcaccgacat cgcggcgacg gtcgatttcc
     4081 gcgtcttcaa agagattgcc caggccggca agaaagtccg cggcatccgc gcgcccggcg
     4141 gggcgagttt ctacagccgc aagctgatcg acgagctgac cgagttcgtc aagcagttcg
     4201 aggccaaggg tctggcgtgg ttccgcgtcg agccggatgg caagctgttc tcgccgatcg
     4261 ccaagaattt caccgaggag cagctcggca agatcaagga gcggatgcga gccgcaccgg
     4321 gcgacatcct gtttttcgtc gccgacacct gggcggtgag ctgcaaggcg ctgcacaacc
     4381 tgcgcaagcg ggtgggcgtc gatctgaagc tatacgaccc taaggcgatg aacttttcct
     4441 ggatcgtcga attcccgatg ttcgagttcg acaaggaaga gaaccgctgg gcctcgatgc
     4501 accacccgtt caccgcgccg cggccgcagg acgttcccat gctcgacagc gacccgggcc
     4561 aggcccgcgc ggtcgcctac gacctggtga tcaacggcag cgaggccggc ggcggcacga
     4621 tccgcattca cgaccaggcc acgcagtcga aggtgttcgg cctcttgggg atcgacgccg
     4681 agacggccaa gaaccgcttc gggtttttgc tcgatgcgct gcagatgggc gcgccgcccc
     4741 acggcggcat cgccctgggc atcgaccgca tcgtcatgct cttcggcacg ctggaaaaca
     4801 tccgcgaggt
//
LOCUS       DASRJB010000899        12246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1121313, whole genome shotgun sequence.
ACCESSION   DASRJB010000899 DASRJB010000000
VERSION     DASRJB010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121313"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VFB80_23680"
     CDS             <1..1372
                     /locus_tag="VFB80_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36859.1"
                     /translation="PYGQPDSRQTVGGLDSGATYDPEHELAYTFGGQSSSGGKNALFS
                     YDAYSNELTFLDAANKPPARDGMGLAYDTRHNKLVMFGSQYLEDERTWLYDPEKNAWE
                     SHLLQPHPPHQKVTKDYCTIPRLAYDPLHDVVLCLAWMGEGGHETWALDVGQLQWTKL
                     SPAVEPAGSKSRSRNLDFDVERNLFILETSSSKTNRPEIWTYRYAPADDAARSALAKR
                     GGGRTRTQPRVLLEPVVSLLDGDQVEVAWQKHPAEDVVGYNVYRGIASVRTVKKGSPG
                     AWKDNDPEYAEPQVVQVRDIVRIEKLNEQPLKELKLVDKVDLTRRLPESEDYRLAVYA
                     YIVRAVNRAGVESGPSPYALTLPSEPRNVLCREDGTAAELKWDAAREREIAGYHVYKL
                     KGTWEIVRVTEQPITETTFRHEAGKDKTRYWVVAVDRLGQEGQPSTPAWFNQVHRGFF
                     EGQWHQ"
     gene            1632..2435
                     /locus_tag="VFB80_23685"
     CDS             1632..2435
                     /locus_tag="VFB80_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36860.1"
                     /translation="MGVTCDLRLIDHRQVVEGGLVAVNRALAAKDPEILREYIAGMQF
                     TPDPAAVQSREARLQRLKEAQAPEMIVQNEERLLSLVNGEAYSAAALAGLSLGELREM
                     LGQWGWAAQSYSLDKAWYELDWFLQPREGQGELLLHPSRPPVGDPQQTLLDQALHGAE
                     PSPLDDSRSSIIRTCGSQQPDCFGYNHPSKAAQISAQLEQIDPESWEDVVPRRAALYQ
                     RATPSLDTETIEATVAQELEFARDAFDVMRRAYAAAAAGQFGVACEYSL"
     gene            2457..4052
                     /locus_tag="VFB80_23690"
     CDS             2457..4052
                     /locus_tag="VFB80_23690"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN36861.1"
                     /translation="MNLTALLAVAAGLLAHDHTAHLFVKAQAAQQAVQKQQAEPAPAA
                     DDKNAVQVTLTLELDKRSVGGLVRITNLASGKPLSFRDEIHRAEQWYTLPAGATLSVP
                     RARLKVEAFHGLETELFATEVDPGERKTVEVKCPLVRFYSARERGLVAGNTHLHLMKL
                     THAEMDRYLRLTPAGDGLDLVFVSLLRRIPDERDYITNTLTEGDFGRLSQGAVKFGNG
                     QEHRHNFGTGGEGFGHVMLLNILKRIEPVSIGPGIMREGTDGIPLQRGIKTAREDGAT
                     VIWCHNTFGHEDIPNWIGGLLHAQNIFDGGDHGGYADTYYRYLNVGMKVPFSTGTDWF
                     IYDFSRVYVPLAGELTPQRWLAELAAGRSYITNGPLLELSAAGQPIGGTIAAKAGDEI
                     RIQGRAIARQEFRRVELIHNGAVVYSAAAKRDAGHSSASVEHTLRISGSGWLALRVPD
                     DAGQSELGKPLFAHTSPIYIEVGGRRLFQPEVARGMLKEVQASVAKIQQQAVFANNEE
                     REAVLRVYRDAAAAWQKRIDENQ"
     gene            4145..4534
                     /gene="rbsD"
                     /locus_tag="VFB80_23695"
     CDS             4145..4534
                     /gene="rbsD"
                     /locus_tag="VFB80_23695"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HZN36862.1"
                     /translation="MLKTGILNPHVLSLLARVRHTNWLVIADRGFPSWPGLETIDLSL
                     VDDVPTVVQVLAAIRPNFVIAEAWMAEEFQRENTPASQARFAEALAGVRVSYEPHVTF
                     KQRVPRAIGLIRTGDTVQYANVILESG"
     gene            complement(4449..6263)
                     /locus_tag="VFB80_23700"
     CDS             complement(4449..6263)
                     /locus_tag="VFB80_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN36863.1"
                     /translation="MQNHQPPAVKDESSWVERLRSDVLAGFLVFLIALPLCLAIAKAS
                     GFPEIAGVFTAIVGGLVTPWLSNSQLTIKGPAAGMIVIVLGAVTDFGFTPGPTTPEAI
                     AGNLAAYKMALAVGVVAAGIQILFGLFKAGSLGDFFPSSAVHGLLAAIGVIIISKQVP
                     VALGVVPHAKEPLELLAEIPSELQHLNPEIAAIGFVSLLILFLFPLIKNSFLRKIPAP
                     MIVLAAAVPLGLYFNLSQQHDYTVLGHHYTVGNKFLVNVPASLASAVTFPDFSVFTNT
                     ALIWKALKWVVMFSLIGSLESMLSAKAVDILDPQRRKTNLNGDLLAVGFANLVSSAIG
                     GLPMISEIVRSRANIDNGAKSKFANMFHGIFLLLAVAFAAPAIRMIPLAALGAMLVYT
                     GFRLAHPREFLHVYKIGREQLVVFVGTIIAVLATDLLIGIFIGIGIELLIQFINGVPL
                     VSLFRPDAEVRQIDERTWLICPRRSAVFFNWLPIRSKIERYGLAQNMNVIVDLSQTQL
                     VDHTVMEKLHQLEREFAERGIALAIVGLDDHVQLSEHPAAARRAVRRRRPARPMPPRL
                     QPSARKSWSRTSPIPGSRWRTAPCLRCGSARWPVARAA"
     gene            complement(6361..7809)
                     /locus_tag="VFB80_23705"
     CDS             complement(6361..7809)
                     /locus_tag="VFB80_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN36864.1"
                     /translation="MNLDLPLLELAILIPLAGAAIVSRIGNSDQARLWCLGFCGAAFA
                     CTVGEWLDFHTLHAEIAHDPGLLARSMGDFLEIDALSAPLLPLTALIYLLTAIATLRT
                     KIRRFSFAGTLLSLALALAVLSCRNPWGIIGLLALRTVAPYLELRARGKPTRVYVLHM
                     ALFVVTLAAGWLFVELEGLHRAHSLWAVLPLLIAVLIRTGGFPFHCWVTDLFEHATFG
                     TALLYLTPMMGAYVAVRLVVPIAPEWVLQSIGLISLATAVYSAGMALVQREARRFFSY
                     IYLSHSALVLVGLETLNPIGLTGALCVWLSAALSLAGFGLTLRALEARHGRLSLTNYH
                     GVYEHTPLLAVCFLLTGFACVGFPGTFGFLGTELLVDGAVQTFPHIGVAVVIAAALNG
                     IAVVQAYFKLFTGTRHVSTVPLQIGLRERLAVLSLAALILGGGIFPQLIVPARHHAAE
                     RILSQREPPKPAGLHPGETAQFSAKSRPPKPK"
     gene            complement(7806..9206)
                     /locus_tag="VFB80_23710"
     CDS             complement(7806..9206)
                     /locus_tag="VFB80_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN36865.1"
                     /translation="MFHAIALTPLNLLGLTVVAVPVLLVAALGLPFLYGRPLSERWIG
                     RIVQSGMIVGLLASLGVLGLMLAQGTRSVTLDLGHWVSLPHFHFAFKFVFDRLSVPFA
                     ILSFVLCGTIGAFANKYLHREPGFARFFMQYALFVMGMIFTSLAGTIESLFFGWELVG
                     VSSAMLVAFFHERTAPVRNGLRVWIVYRISDAALLVAAVVLHHLRGEGDFSALMGIYP
                     WPESHTVLTGNEPLIIGLLLLVAAAGKSALVPFSGWLPRAMEGPTPSSAVFYGALSVH
                     LGAYLLLRVSPLLDASPTLSALVLILGLVSAMFGALASRAQTDVKCALAFASLTQVGL
                     IVAEIGAGLPRYIPLVHILGHACLRTLQFLRASSVLTDFRQLENAIGSRLPPFSSPLG
                     RFVPAALRVPLYRLAVQRGYLDALLFEYIARPVLGLFRLCDSLERRWTDFLSGGQSRE
                     SDRALGAASTLDDLSL"
     gene            complement(9215..>12246)
                     /locus_tag="VFB80_23715"
     CDS             complement(9215..>12246)
                     /locus_tag="VFB80_23715"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HZN36866.1"
                     /translation="SERAERLGRLRKALEHAAHYLPAQGPIKVFIHHNTLHAFEDLPF
                     DAGVQAGAKLFGCQPYLAEDRYREMLATGRIQHADLFVALLDVQGERADDLLGFMGTR
                     FHFRLAMLQHPLRVAPAAELRWFVAETDALTRMRDDAPPEVRERFLRETRHWVLRDLR
                     GKPEATPTCLHDVFERFGRATLESWSDRKWEAFTLQALWRICRAGVHGLKAHAGPPAP
                     GTRHHELLREATGSDCDQLVHELLIPFCSAYLDQGYSHWPLPERDAGLFHSFAAVYGR
                     EGNLHQAWLEDLSTELARITQAGISPEESILESLELLGVEPSEWESFVTSTLLALRGW
                     AGMIRQVEIRRDSVAHPIPEGSLGELLAVRLILDRLAAAWVAKETLGYDGPLADLRRV
                     VRATGSKPGTSGVEQRAFQVFQLAQALGWLPQHLASLSKREWIDLVLEIEDFPAIKRR
                     QVWHAAFERRHRVQTLDAIALHSRRPAARVPQPRFQVICCLDEREESFRRHLEEVAPE
                     VETFGAAGFFNIPIYYRGAADAHYVPLCPIVIKPQHWVEEDVCYTHDEAHATRARFRR
                     ALGMASHQMHRSSLTAAGGALLTAGLGALASIPLVARVLFPRLTARIRSLAGSMVEPP
                     PQTRLELERSQPTPGPEAGQVGFTLDEMANMGERLLRDIGLTSSFARIVVALGHGSNS
                     LNNPHISAYNCGACGGGCGGPNGRSLAHILNDPRIRERLKQRGLVIPDDTIFIGGWHN
                     TCNDYVNYFDLDRLPKSHKAEFDRVRREIEEACDRNAHERCRRFISAPLNLSFPAARR
                     HVEARSEDLAQTRPECGHASNALCIVARRSRTRGLYLDRRAFLTSYDPTQDDEQTSTL
                     VRLLSAAVPVCAGINLEYYFSYVDNVGYGCGTKLPHNVTSLLGVMEGMTSDLRPGLPW
                     QMVEIHDPVRLLFIIETTPQKMDSLLDRFPAIGKLCRNGWVQVATLDPDSTAIHVLKN
                     GRFEPYRPESTELPKVAASVDWYRGWRENLGYAQIET"
BASE COUNT         2307 a         3909 c         4162 g         1868 t
ORIGIN      
        1 gccgtatggc cagccggatt cgcggcaaac ggttggcggg ctggatagcg gcgcgaccta
       61 cgatcccgag cacgagctgg cttatacgtt cggcgggcag tcctccagcg gcggcaagaa
      121 cgccctcttc tcctatgacg cttacagcaa cgagctgacg tttctcgacg cggcgaataa
      181 gccgccagcc cgcgacggca tgggcctggc ctatgacacg cggcacaaca agctcgtcat
      241 gttcggcagt cagtatctcg aagacgaacg gacctggctc tacgatcccg agaagaacgc
      301 ctgggagagc catttgcttc agccccatcc gccgcatcag aaggtgacga aggactattg
      361 cacgatcccg cggctggcct acgacccgct gcacgacgtg gtcctctgcc tggcctggat
      421 gggcgagggc ggacacgaaa cgtgggccct cgacgtgggc cagctgcagt ggacgaagct
      481 cagccccgcc gtcgaaccgg ccggcagcaa aagccgctcg cggaacctcg acttcgacgt
      541 ggagcggaac ctgttcatcc tggaaacgtc gtcgtcgaag accaaccggc cggagatctg
      601 gacgtatcgc tacgccccgg ccgatgacgc ggccaggagc gccttggcca aacgtggcgg
      661 cggacgcact cgcacgcagc cgcgggtgct gctggagccg gtggtctcgc tgctggacgg
      721 tgaccaggtg gaagtcgcct ggcaaaaaca cccggcggag gatgtcgtcg gctacaacgt
      781 ctatcgtggg atcgccagcg tgcgaaccgt gaagaaaggc tcgcccggcg cctggaaaga
      841 caacgacccc gaatatgccg agccgcaggt cgtgcaggtt cgcgacatcg tcaggatcga
      901 aaagctcaac gagcagccgc tgaaggagct gaagctcgtt gacaaggtcg atctgacgcg
      961 gcggctgccg gagtcggagg attatcgcct ggccgtctat gcctacatcg tgcgtgcggt
     1021 caaccgggcg ggcgtcgaga gcgggccatc gccgtacgcc ctgacgctgc catcggagcc
     1081 gcgcaacgtg ctgtgccgcg aggacgggac tgcggccgag ctgaagtggg acgccgcccg
     1141 cgagcgcgag atcgccggct atcacgtcta taagctgaaa ggaacgtggg agatcgtccg
     1201 cgtgaccgag cagccgatta cggagacgac gttccggcac gaagcgggca aggacaagac
     1261 gcgatactgg gtcgtcgcgg tcgatcggct aggacaagag ggccagccct caacgcccgc
     1321 ctggttcaac caggtccacc gcgggttttt tgagggccag tggcatcagt agcgtggtcc
     1381 tgaccttccc cacgaaccgt tgggaaggtc atcctactac tcttactctt gatcctgctc
     1441 ctaatcctct tcctaatcat aatcctcctc ttgctcttcc tccgaggcgt cgagggttga
     1501 tctccagccg cgcacaaagg cggctacgga gcaagagcag gaggaagagg aagattagga
     1561 acaggattaa gagcaagatt aggattagga gcaggatcgg gcgtcggagt ccgtgagggc
     1621 caaggcgaat catgggcgtc acttgcgacc tgcgactgat cgatcaccgg caggtcgtgg
     1681 aaggcgggct agtggcagtg aatcgcgcgc tggcggccaa ggacccggag attctgcggg
     1741 aatatattgc cgggatgcaa ttcacgcccg atccggccgc cgtccaaagc cgcgaggccc
     1801 ggctgcagcg cctcaaagag gcgcaagcgc cggaaatgat tgtccagaat gaggagcggc
     1861 tcttgtcgct ggtcaacggc gaggcctatt cggccgccgc gctggcagga ctttcgctgg
     1921 gcgagctgcg cgagatgctc ggccaatggg gttgggcggc ccagtcgtac agcctcgaca
     1981 aggcgtggta tgaactggac tggttcctgc agccgcgcga ggggcaggga gagctgctgc
     2041 ttcatccgtc gcgcccgccc gtcggcgatc cgcagcaaac gctgctcgac caggcgctgc
     2101 acggggccga gccgtcgccg ctcgacgact cgcgctcttc gatcattcgc acctgtggat
     2161 cgcagcagcc cgactgtttt ggctacaacc atcccagcaa agccgcccag atcagcgcgc
     2221 agctggagca gattgacccg gagagctggg aggacgtcgt gccgcggcgg gcagcactct
     2281 accagcgagc aacgccgagc ttggacacgg agacgattga agccacggta gcccaggagt
     2341 tagagttcgc ccgcgatgcg tttgacgtga tgcgccgggc ctacgccgcg gcggccgcgg
     2401 gtcagttcgg cgtcgcctgt gagtacagcc tttaagaaac accttggggg actgtcatga
     2461 atctgactgc tttgctggcc gtcgccgcgg gcctcttggc tcacgaccac accgctcatt
     2521 tgttcgtgaa ggcccaggca gcgcagcaag ccgtgcagaa gcagcaggcg gagcctgcgc
     2581 cggcggcgga cgacaagaat gcggtgcagg taacgctcac gctggagttg gacaagcgaa
     2641 gcgtgggcgg tctggtgcgg atcacgaatc tggccagcgg gaagccgctg tcgttccgcg
     2701 acgagattca ccgggccgag cagtggtaca cgctgcccgc cggtgcgacc ttgagcgtgc
     2761 cgcgggccag gctgaaggtc gaggcgtttc acggcctgga gacagagctg ttcgccaccg
     2821 aagtcgatcc gggcgagcgg aagacggtcg aggtgaagtg cccgctcgtg cggttctaca
     2881 gcgctcgcga gcgagggctc gtcgccggca acacgcacct gcatctgatg aagctgacgc
     2941 acgccgagat ggaccgctat ctccgcctga cgccggcggg cgatggcctg gatctggtgt
     3001 tcgtctcact gctgcggcgg attcccgacg agcgggacta catcaccaac acgctcaccg
     3061 aaggcgattt cggccggctc tcgcaagggg ccgtcaaatt cggcaatggc caggagcacc
     3121 gccacaattt tggcacgggc ggcgagggct ttggccacgt gatgctgctg aacatcttga
     3181 agcggatcga gccggtcagc atcgggccgg gcatcatgcg cgaagggacc gacggcattc
     3241 ccttgcagcg cgggatcaag acggcccgcg aggacggcgc gaccgtcatc tggtgccaca
     3301 atacgttcgg gcacgaagac attcccaact ggatcggcgg cctgctccac gcccagaaca
     3361 tcttcgacgg cggcgatcac ggcggctacg ccgacacgta ttaccgttac ctcaacgtcg
     3421 gaatgaaggt cccgttctcg accggcaccg actggttcat ctacgacttc tcgcgggtct
     3481 atgtgccgct ggccggcgag ctgacgcctc agcggtggct cgccgaattg gcggccggcc
     3541 ggtcgtatat caccaacggg ccgctcttgg agctgagcgc cgccggccaa ccgatcggcg
     3601 gcacgatcgc ggcgaaagcg ggcgacgaaa tccggattca gggccgggcc atcgcccggc
     3661 aggaattccg ccgcgtggag ctgatccata acggcgcggt cgtctattcg gcggccgcca
     3721 agcgcgacgc aggtcattcc tcggcgagcg tcgagcacac gctgcggatc agcggctccg
     3781 gctggctggc cctccgcgtt cccgacgacg ccggccagag cgaactgggc aaaccgctct
     3841 tcgcccacac cagcccgatc tacatcgaag tcggcggccg ccggctcttt cagcccgaag
     3901 tggcccgcgg gatgctgaag gaagtgcagg ccagcgtggc caagattcag cagcaggccg
     3961 tgtttgccaa taacgaggag cgcgaggcgg tcctgcgcgt ctatcgcgac gcagccgcgg
     4021 cctggcaaaa acggatcgat gagaaccagt agatcggtca gtcggccagt cggtccgtcg
     4081 gttgttcggc tgcctgacct aaccgagcta ccgaccactg accaccgacc gaccgtccta
     4141 agccatgctc aaaacgggaa tcctcaatcc gcacgtcctc tcgctgctgg cgcgggtgcg
     4201 gcatacgaac tggctggtga ttgcggaccg cggcttcccg tcgtggccgg gtctggagac
     4261 gatcgacctg tcgctggtcg atgacgtgcc gacggtggtg caggtgctcg cggccatcag
     4321 gccgaacttc gtcatcgccg aggcctggat ggccgaagaa ttccagcggg agaatacgcc
     4381 ggccagtcag gcccgctttg ccgaggcgct ggccggcgtc cgcgtcagct acgagccgca
     4441 cgtgaccttc aagcagcgcg tgccacgggc catcgggctg atccgcaccg gagacacggt
     4501 gcagtacgcc aacgtgatcc tggaatcggg ctagttcgcg accagctctt ccttgcgctc
     4561 ggctgcaacc gcggcggcat gggccgcgcg ggtcggcgtc gtcggaccgc gcggcgggcg
     4621 gcggcggggt gctcggaaag ctgcacgtgg tcgtccaggc cgacgatcgc cagggcgatg
     4681 ccccgctcgg cgaactcgcg ctccagctgg tgcagctttt ccatcaccgt atgatcgacc
     4741 agctgcgtct gcgacagatc gacgatgacg ttcatattct gggccaggcc gtagcgctcg
     4801 atcttgctgc gaatcggcag ccagttgaag aacacggcgc tccgccgcgg gcagatcagc
     4861 caggttcgct cgtcaatctg ccgcacctcg gcgtcgggcc ggaagagcga caccagcggt
     4921 acgccgttga tgaactgaat cagcagctcg atgccgatgc cgatgaagat gccgatcaag
     4981 aggtccgtcg ccagcacggc gatgatcgtg cccacgaaca ccaccagctg ctcgcggccg
     5041 atcttgtaga cgtgcaggaa ttcgcgggga tgggccaggc ggaagcccgt gtagacgagc
     5101 atcgcgccga gcgcggccag cgggatcatg cggatcgccg gcgccgcgaa cgccaccgcc
     5161 agcagcagga aaatgccgtg gaacatgttg gcgaacttgc tcttcgcgcc gttgtcgata
     5221 ttggcccggc tgcggacgat ttccgaaatc atcggcaggc cgccgatcgc cgagctgacc
     5281 aggttggcga agcccacggc caggaggtcg ccgttgagat tcgtctttcg ccgctgcgga
     5341 tcgagaatat cgacggcctt ggcgctgagc atcgattcca ggctgccgat cagcgagaac
     5401 atcaccaccc atttcagggc cttccagatt agggcggtgt tcgtgaacac cgagaaatcg
     5461 gggaacgtga ccgcactagc caggctggcg ggaacgttga ccaggaactt gttgccgacc
     5521 gtgtagtgat gaccgagcac cgtgtagtcg tgctgctggc tcaagttgaa gtacagtccc
     5581 agcggcaccg ccgccgccag cacgatcatc ggcgcgggaa tcttgcgaag gaacgagttt
     5641 tttatcagcg gaaacaggaa caggatcagc aggctgacaa acccgattgc ggcgatttcg
     5701 ggattcaggt gctgaagctc tgagggaatc tccgccagca gctccaatgg ctccttggcg
     5761 tgtggtacta cgcccagcgc aaccggcacc tgcttgctga tgatgatcac gccgatcgcc
     5821 gccagcaggc cgtgaacggc cgacgacggg aagaaatcgc ccagcgagcc ggccttgaac
     5881 aaaccaaaca ggatctgaat gccggccgcg acgacgccga cggccagggc catcttgtag
     5941 gccgcgaggt tgccggcaat cgcctccggc gtggtcggtc ccggcgtgaa gccgaagtcg
     6001 gtcaccgcgc cgagcacgat cacgatcatg ccggccgccg ggcccttgat cgtgagctgg
     6061 ctgttgctga gccagggagt taccaagccg ccgacgatcg ccgtgaagac gccggcgatt
     6121 tcgggaaagc cgctggcctt ggcgatcgcc aggcagagcg gcaaggcgat caggaacacc
     6181 aggaagccgg ccaggacgtc gctccgcagg cgctcgaccc acgagctttc gtccttcacc
     6241 gcgggcggtt ggtgattttg catgggaggg tttggattcg tgcgagctac cggggattca
     6301 gaatacttgt ttcctcgaaa tcgtcccgcc aagttgacgg gtttgtaatt cggccggcat
     6361 tcacttgggt ttcggcggac gcgactttgc cgaaaattgc gccgtttcgc caggatgcaa
     6421 accggcgggt ttcggcggct cgcgctggct gaggattcgc tcggcggcat ggtgccgcgc
     6481 gggcacgatc agctgcggga aaatgccacc gcccagaatg agcgcggcga gcgacaggac
     6541 cgccagccgc tcgcggaggc cgatctgcag cgggacggtg gatacgtgcc gcgtgccggt
     6601 gaacagtttg aaataggcct gcacgacggc gatgccgttc agtgcggcgg caatcacgac
     6661 agccacgccg atgtggggaa aggtctgcac ggccccatcg acgagcagct cggtgcccag
     6721 aaaaccgaaa gtgccgggaa agccgacgca ggcaaagccc gtcagcagaa agcagacggc
     6781 cagcagcggc gtgtgctcgt agacgccgtg atagttcgtc agcgagaggc ggccgtgccg
     6841 ggcttccagg gcgcggagcg tcaggccaaa acccgccagc gacagcgcgg cggagagcca
     6901 cacgcacagc gctccggtca ggccgatcgg attgagcgtc tccagcccga ccagcaccag
     6961 cgcggaatgg ctcagataga tgtatgagaa aaaccgccgg gcttcgcgct gcacgagggc
     7021 catgccggcg ctatagaccg cggtggccag cgaaatcagg ccgatgcttt gcagcaccca
     7081 ctccggcgcg atcggcacga ccaggcggac cgcgacgtag gcccccatca tcggcgtcag
     7141 gtagagcagg gcggtgccga aggtggcgtg ctcgaacagg tcggtcaccc agcagtgaaa
     7201 cgggaagccg ccggtgcgga tgagcacggc aatcaagagc ggcagcacgg cccacagcga
     7261 gtgggcccgg tgcaggccct ccagctcgac gaacagccag ccggcggcca gcgtcacgac
     7321 gaacagcgcc atgtgcagga cgtagacccg cgtcggcttg ccgcgggccc gcagctccag
     7381 gtagggcgcg accgtccgca gcgccagcag gccgatgatg ccccaaggat tgcggcagct
     7441 gagcactgcc agggccagag ccagcgagag cagcgtgccc gcgaacgaga agcggcggat
     7501 cttcgtgcgg agcgtagcga tcgcggtcaa gagatagatg agcgccgtca gcggcaggag
     7561 cggggcgctg agggcgtcga tttccagaaa gtcccccatc gagcgggcca agaggcccgg
     7621 gtcgtgcgca atctcggcgt gcagcgtgtg aaaatccagc cactcgccga cggtgcaggc
     7681 aaacgccgcc ccgcaaaatc ccaggcacca caggcgggcc tgatcggagt tgccgatccg
     7741 cgatacgatc gccgcccccg cgaggggaat caggatcgcc agctccaaga gtggaaggtc
     7801 caggttcata gcgacagatc gtccagcgtg ctggccgctc ccagggcgcg atcggactcg
     7861 cgcgattggc cgccggaaag aaaatcggtc cagcgccgct ccaaggagtc gcacaagcgg
     7921 aacaggccga gcaccggccg ggcgatgtac tcgaagagca gggcgtcgag gtacccgcgc
     7981 tgtacggcca gccgatacag cggcacgcgc agcgcggcgg ggacgaaccg gcccagcggg
     8041 ctgctgaacg gcggcagccg cgagccgatg gcgttttcca gctggcggaa gtcggtgagc
     8101 acgctgctgg cccgcagaaa ctggagcgtc cgcaggcagg cgtgccccag gatgtggacg
     8161 agggggatgt accgcggcag gccggcgccg atctccgcca caatcaggcc gacttgcgtg
     8221 agcgaggcga aggccagcgc gcacttgacg tcggtctggg cccggctggc cagcgcgccg
     8281 aacatcgcgg aaacgaggcc cagaatcagc accagggcgc tgagcgtcgg cgaagcgtcg
     8341 agcagcgggc tgacacggag cagcaagtag gcgcccagat gcaccgacag ggccccgtaa
     8401 aacacggcgc tggagggagt cggtccttcc atcgcccgcg gcagccagcc cgaaaacgga
     8461 accagcgccg atttcccggc cgcagccacg agcagcagca ggccgatgat cagcggctca
     8521 ttgccggtga gaacggtgtg gctttccggc cagggataga tgcccatcaa ggccgagaag
     8581 tccccctcgc cgcgcaaatg gtgcaaaacc accgccgcca cgagcagcgc cgcgtcggag
     8641 atgcgataca cgatccagac ccgcagcccg ttccgcacgg gcgccgtgcg ttcgtgaaag
     8701 aacgctacca gcatggcgga cgacacgccg accagctccc agccgaagaa cagcgattcg
     8761 atcgtgccgg ccagcgaggt gaagatcatg cccatcacga acagggcgta ttgcatgaag
     8821 aaccgggcga agcccggctc gcgatgcagg tacttgttgg cgaatgctcc gatcgtgccg
     8881 cagagcacga acgacaggat cgcgaacggc accgacaggc ggtcgaacac gaacttgaac
     8941 gcgaaatgaa agtggggcag ggaaacccag tgccccaagt ccagcgtcac cgagcgcgtg
     9001 ccctgagcca gcatcagccc cagcactccc agcgaagcca ataagcccac gatcatcccg
     9061 gattgcacga ttcgcccgat ccaccgctcg ctgagcgggc ggccatacaa aaacggcaga
     9121 cccagcgccg ccaccagcag cacgggaacg gcgaccacgg tcagtcccag caagttgagc
     9181 ggcgtcagtg cgatggcgtg gaacatgaaa ggcatcaagt ttcgatctgg gcgtacccga
     9241 ggttctcgcg ccagccgcgg taccaatcga ccgaggcggc cactttcggc aactcggtgg
     9301 attcaggccg gtaaggctcg aagcggccgt tcttcagcac gtgaatcgcg gtcgagtccg
     9361 ggtccagcgt cgcgacctgc acccagccgt tgcggcacag cttgccgatg gccgggaagc
     9421 ggtccagcag cgagtccatc ttttgcggcg tcgtctcaat gatgaacaac agccgcaccg
     9481 ggtcgtgaat ctcgaccatc tgccagggca gccccggccg caggtcgctg gtcatccctt
     9541 ccatcacgcc caggagcgac gtgacgttgt gcggcagctt cgtgccgcag ccgtagccga
     9601 cgttatcgac gtaggagaag tagtattcga gattgatgcc cgcgcagacc gggacggcgg
     9661 cgctgagcag ccgcaccagc gtgctggtct gctcgtcgtc ctgcgtgggg tcgtacgacg
     9721 tgaggaacgc ccggcggtcg agatacagcc cccgcgtgcg gctgcggcgg gcgacgatgc
     9781 acagcgcgtt gctggcgtgt ccgcattcgg ggcgggtctg ggccaggtct tccgagcggg
     9841 cttcgacgtg cctgcgggcc gcgggaaacg acaggttgag cggcgcggag atgaaccgcc
     9901 ggcagcgttc gtgggcgttg cggtcgcagg cttcctcgat ctcccgccgc acccggtcga
     9961 attcggcctt gtgcgacttg ggcaggcggt cgaggtcgaa gtagttgacg tagtcgttgc
    10021 aggtgttgtg ccagccgccg atgaagatcg tgtcgtcggg gatgaccagg ccgcgctgct
    10081 tcagccgttc gcgaatccgc gggtcgttca agatatgggc cagcgaacgg ccgttgggtc
    10141 cgccgcagcc gccgccgcaa gcgccgcagt tgtaagccga gatgtgcgga ttgttgaggc
    10201 tgttcgagcc gtggccgagg gccacgacga tccgggcaaa gctgctggtc aggccgatgt
    10261 cgcggagcag ccgctcgccc atgttggcca tttcgtcgag ggtgaacccg acttgccccg
    10321 cttccggacc cggcgtgggc tgcgaccgct ccagttccag ccgcgtttgc ggcgggggct
    10381 cgaccatgct gccggccaga ctgcgaatcc gcgcggtcag ccgcggaaag aggacgcggg
    10441 ccaccagcgg gatcgaggcc aaggcgccga ggccggccgt gagcagcgcg ccgccggccg
    10501 ccgtgaggct gctgcgatgc atctggtgcg acgccatgcc cagggcgcgg cggaatctgg
    10561 cccgggtggc gtgggcctcg tcgtgcgtgt agcagacgtc ttcttcgacc cagtgctgcg
    10621 gcttgatgac gatcgggcag agcggcacgt agtgggcgtc ggcggccccg cggtagtaga
    10681 tcgggatgtt gaagaacccc gccgccccga acgtctccac ctcgggcgcg acctcttcca
    10741 gatgccggcg gaacgactcc tcgcgctcgt ccaggcaaca gatcacctga aatcgcggct
    10801 gcggcacgcg tgctgccggc cgccgggagt gcagggcaat cgcatcgagc gtttgcacgc
    10861 ggtgccttcg ctcgaacgcg gcgtgccaga cctgcctccg cttgatcgct ggaaaatcct
    10921 cgatttcgag gacgaggtcg atccactccc gcttcgagag cgacgccaga tgttgcggca
    10981 gccagcccag cgcctgggcg agctgaaaga cctgaaacgc ccgctgctcg acgccggaag
    11041 tcccgggctt cgatccggtc gcgcggacca cccgccgcag gtcggcgagc gggccgtcgt
    11101 agcccaaagt ttccttggcg acccaggccg ccgccagccg gtccagaatc agccggacgg
    11161 ccaggagttc tccgaggctg ccctcgggga tcgggtgggc tacgctgtcg cggcgaattt
    11221 cgacctggcg gatcatgccg gcccagccgc ggagagccaa aagagtggac gtgacaaagc
    11281 tctcccactc ggacggctcc acgcccagca gctcgagcga ttcgaggatc gattcttctg
    11341 ggctgattcc cgcttgcgtg atgcgcgcca gctcggtcga taaatcctcg agccaggcct
    11401 ggtgcaggtt gccctcccgg ccataaacgg ccgcgaagga gtgaaacagg cccgcgtccc
    11461 gctcgggcaa gggccagtgc gaatagccct ggtccagata cgcggaacaa aaaggaatca
    11521 gcagctcgtg caccaactgg tcgcagtcgc tgccggtagc ttcgcgcaag agctcgtggt
    11581 gccgcgtgcc cggggcgggc ggtcccgcgt gagctttcag cccgtgtacg ccggcccggc
    11641 agatccgcca cagggcctgc agcgtgaagg cctcccattt gcggtcggac caggattcga
    11701 gcgtcgcccg cccaaaacgc tcgaacacgt cgtgcaggca ggtcggcgtc gcttccggct
    11761 tgccgcgcag gtcgcgcagc acccagtgcc gcgtttcacg caggaaccgc tcgcggacct
    11821 ccggcggcgc gtcgtcccgc attcgcgtca gggcgtcggt ctcggccacg aaccaccgca
    11881 gctcggcggc cggcgcgacg cgcagcgggt gctggagcat cgccagacgg aaatgaaacc
    11941 gcgtccccat gaagccgagc aggtcgtcgg cccgctcgcc ctgtacgtcg agcagcgcga
    12001 cgaacaagtc cgcgtgctga atgcggcctg tcgccagcat ctcgcgatag cggtcttcgg
    12061 ccaggtacgg ctggcagcca aacagcttcg ctcccgcttg gactcccgcg tcgaacggca
    12121 ggtcctcgaa ggcgtgcagc gtgttgtggt ggatgaagac cttgatcggc ccctgggcgg
    12181 gcaggtaatg cgcggcgtgc tccagggctt tccgcagccg gccgaggcgc tccgcacgct
    12241 cggaga
//
LOCUS       DASRJB010000900         7904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_558289, whole genome shotgun sequence.
ACCESSION   DASRJB010000900 DASRJB010000000
VERSION     DASRJB010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558289"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFB80_23720"
     CDS             <1..595
                     /locus_tag="VFB80_23720"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36867.1"
                     /translation="VGGKTVFNHVLKVHLCPSEQSSRGGQGATTNGRADLWAIGNYAA
                     NAYVFGYMRGTNANARRETPQRFGDLVDGTSNVIMMTERFGTCGNGGSPNSAQTFGNL
                     WSDSNSVWRPIFCLANQGKDPPAIAGGAAYPDCEKFQVMPHWFNTCLSTRAQSPHPAG
                     ILVSLADGSVRFISQSIPDKQWAIACDPQDGVNASDL"
     gene            614..1000
                     /locus_tag="VFB80_23725"
     CDS             614..1000
                     /locus_tag="VFB80_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36868.1"
                     /translation="MRTLLLIAAAMLFFVGCGGGSASPAPTTHPVSGKLLSKAGRPVT
                     EGSIEFVNIGPTPVRATADVSADGSFSLTTMTADGKKYPGAEVGDYKVTWYPVMKSES
                     QSEVPVALRGTQKIVAGSNELNLKLP"
     gene            1064..2527
                     /gene="cls"
                     /locus_tag="VFB80_23730"
     CDS             1064..2527
                     /gene="cls"
                     /locus_tag="VFB80_23730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZN36869.1"
                     /translation="MVEFFGWFFSTIYPWLIGLVHVVVMAFASGHVVLTKRDSRSAIG
                     WVGIIWLTPIIGSILYFMFGVNRIQRKAKTLMRRHGQTESSAGRRPPTDDVLLHVLGE
                     DAMHLAALGRYVSKLIHLPLVDGNRLTPLTSGRDAYDQMLAAVDSAQTSVALMTYIFD
                     NDAAGRLFAEALGRAVQRDLQVRVLIDAVGARYTFPSIKRVLREHGVPYAMFLPTLIP
                     GKLHYSNLRSHRKILVVDGQLGFTGGMNIRQGHLCEPGYPYPVKDLHFRVEGPVVAHL
                     RDAFAVDWEFTTGEHLGGGAWFPTIAPAGHSLCRGIPDGPDLDFDKILLTIHGAIGVA
                     QRSVDIITPYFLPEQPLITALNVAAMRGVAVNIYLPEKNNLRLVQWACAAQLWQVLQR
                     GCRVWLTAPPFDHTKLMIVDGAWAMIGSSNWDPRSLRLNFEFNVECYDRDFAAQLSAL
                     VAAKRASSREITLADVDGRPLWQRLRDGVCRLMLPYL"
     gene            2581..3978
                     /gene="sthA"
                     /locus_tag="VFB80_23735"
     CDS             2581..3978
                     /gene="sthA"
                     /locus_tag="VFB80_23735"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZN36870.1"
                     /translation="MAKNQYDVVVIGTGPGGEGAAMQAVKHGLKVAVVERFAKIGGGC
                     THWGTIPSKALRFAIFQMLEINSSRVFREAGISADVSFPDLRKTAESVIRQQEDMRRG
                     FYERNNVPIYSGHARFVDPHTLEVGEAKGAQERVTADNLVIAVGSRPYRPPDIDFSHP
                     RIFDSDTILNLGFTPWSITVYGAGVVGCEYASMFRNLGCKLNLVNTRAQLLEFLDDEI
                     CDALSYHLREKGALIRHGEQYEKIEGTDDGVILSLKSNKQLKTDILLWANGRTGNTDN
                     LGLETIGIQPDSRGQLKVDDRYATSVPHIYAVGDVIGYPSLASAAYVQGRAAALHIAG
                     DDCARLLANDIPTGIYTSPEISSVGKTERELTAAGVPYEVGHAQFKSLARAQITGQTV
                     GMLKILFHRESRAVLGVHCFGANASEIIHIGQAILAQPPPNNTLMYFINTTFNYPTMA
                     EAYRVAALNGYNRLF"
     gene            3986..4945
                     /gene="gluQRS"
                     /locus_tag="VFB80_23740"
     CDS             3986..4945
                     /gene="gluQRS"
                     /locus_tag="VFB80_23740"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZN36871.1"
                     /translation="MTPPIGRLAPSPTGAQHVGNARTYLLAWLSIRSRGGRVILRMED
                     IDSPRVKRGAAEQALADLKWLGLDWDEGPDIGGPHAPYLQTQRLSLYQEALEKLQAAE
                     RVYPCTCTRSDVAAAASAPHAGQEGPFYPGTCAFRTVADAATLTQNAGEGLPDFAWRL
                     RTTDAARHFVDLAAGFQSCNVAAELGDFVIAKADGTPAYQLAVVVDDHAMGITEVLRG
                     DDLLPSAFRQLELYDFFDWTPPQFAHVPLVVGPDGRRLAKRHGDTRLATLRAAGVAPE
                     RLVGMLAHSCGLIDRPESIPARDLLPSFSLTRLPQEPFVFAGL"
     gene            complement(4896..7265)
                     /locus_tag="VFB80_23745"
     CDS             complement(4896..7265)
                     /locus_tag="VFB80_23745"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN36872.1"
                     /translation="MQRFIELLIAARWPLLLVASVLAAVAFGPSNQVQFDRSIEKMFA
                     PDDPLLPPYQRLKDRFGGNEIVVAVYRDEQLLAGEGQGIERLASISQRMKTVPGVKDV
                     LSLAEVNGLLEQLQGGKIGRGFRRFFPGRPDEWKGPPILNPKSPLAERFRELFSGYTH
                     SADGKIAAVVCMLDPVRPAAAAGDEDPRAATIEALEAIITDLPAGLTPGVLAGEPVMV
                     SDGFALLEKDGRKLGTWTTLLLGLTILVLFRHLRWLMIPIVVVQWTLIVTRAALVVSG
                     LQLSMVSSMLTAIVTVVGVAGVMHLIVEVRELRTQGLTQRAAMLKAGVLLAGPIFAAV
                     LTDVSGFGSLWWASVKPVSDFGTMMVVGSFLVLPAMCLMFPALALAFARDGKKEHGWG
                     EVQLGQWLMRSVDAVQARPKTVAFVTAFIALVASLGAVRLEVETDFTHNFRRGSRIVK
                     AYDFVETNLGGAGVWDVVIPAPPALERDYLSRVRKLEERLRAIQSPAGPPGPALSKVI
                     SIVDAIDAIEADRALAMLSPELRYQGMAAAMPTFAAALLAQKLDENGQARLRIMLRAP
                     ERQPAEQKRWLINEVTRVAREEFPLADDSPGAEVTGFFVLLTNLIESMLRDQWVTFGV
                     SSLAIIIMVIIGYRSWKYALIAMIPNAVPIYVVMGLLGWVGLKMNMGAAMIAAVSMGM
                     SVDSSIHYFSSFRHFRREGKSVHEALTLCQQSVGLAMIFSTIALIVGFGVLITSDFVP
                     TIYFGALMCLAMFGGALGNLIVLPLLLAWTEKDAKGPQTQTAPAAAESN"
     gene            7615..>7904
                     /locus_tag="VFB80_23750"
     CDS             7615..>7904
                     /locus_tag="VFB80_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36873.1"
                     /translation="MIRTLLSFCLLFGGLVAVSKWLHDTTRPAAGDAMPANSSAEPAP
                     APPAAMKPSRLPSSGEAQPAKVRLVAFEDDEPDAPKKNNPVGSTPKTEPTEG"
BASE COUNT         1387 a         2655 c         2507 g         1355 t
ORIGIN      
        1 cgtcggcggc aagacggttt tcaatcacgt gctcaaggtt catctgtgtc ctagcgagca
       61 gagctcgcgc ggcggccagg gcgccacgac gaacgggcga gccgacctgt gggcgatcgg
      121 caactacgcc gccaacgcct acgtcttcgg ctacatgcgc ggaaccaacg ccaatgccag
      181 gcgcgagacg ccgcagcggt tcggcgatct cgtggacggc acgtccaacg tcatcatgat
      241 gaccgagcgt ttcggcacct gcggcaacgg cggctcgccg aactctgccc agacgttcgg
      301 aaacctgtgg agcgattcga acagcgtctg gcgcccgatc ttctgcctcg cgaaccaggg
      361 caaggatcct ccggcaatcg ccggcggggc ggcatatccc gactgcgaga aatttcaggt
      421 catgccgcac tggttcaata cttgtctctc cacccgcgcc cagtcgccgc acccggccgg
      481 gattctggtt tccctggcgg acggcagcgt gcgattcatt tcacagagca tccccgacaa
      541 gcaatgggcg attgcctgcg acccccagga cggcgtgaac gcgagcgatt tgtaaatgac
      601 gcccgggcac ctgttgcgaa cgttgcttct gatcgcggcc gcgatgctgt tcttcgtcgg
      661 ctgcggcggg ggcagcgctt cccctgcacc gacgacgcat ccggtctccg gcaagctgct
      721 ctcgaaggcc ggtcgcccgg taaccgaagg ctcgatcgag ttcgtcaaca ttgggccgac
      781 tccggtccgg gcaacggccg acgtatcggc ggacgggtcc tttagcctga ccacgatgac
      841 cgccgacgga aagaagtatc ccggcgccga agtgggggat tacaaagtga cctggtatcc
      901 cgtgatgaag tcggagagcc agtcggaagt gccggtcgcc ctgcgcggca cgcagaaaat
      961 cgtggccgga tcgaatgagc tgaacctcaa gctgccgtag gccgcggcgg ctggacgctg
     1021 ttttgctctg gcttggcgcc gcctcggcag ccacgatagg gcgatggtcg agttcttcgg
     1081 ctggttcttc tcgacgatct atccgtggct gatcggcctg gtgcacgttg tcgtgatggc
     1141 cttcgcctcc gggcacgtcg tgctgacgaa gcgcgactct cgcagcgcga tcggctgggt
     1201 cggcatcatc tggctcacgc cgatcatcgg ctcgatcctg tacttcatgt tcggcgtcaa
     1261 ccgcatccag cggaaggcca agacgctgat gcgcaggcac ggccagacgg aaagctccgc
     1321 cggccgccgg ccgcccaccg acgacgtgct gctgcacgtc ctgggcgagg acgcgatgca
     1381 cctggccgcg cttggtcggt acgtctccaa gctgattcac ctgccgctgg tggacggcaa
     1441 ccgcctcacg ccgctgacca gcggccgcga cgcctacgac cagatgctcg ccgccgtcga
     1501 ctccgcccag acctcggtcg ccctgatgac ctacatcttc gacaacgacg ccgccggccg
     1561 gctgtttgcc gaagcactcg gccgggccgt gcagcgcgac ctgcaggtcc gcgtgctcat
     1621 cgacgccgtc ggcgcgcggt acacgtttcc ctcgatcaag cgcgtcctcc gcgagcacgg
     1681 cgtcccctac gcgatgtttc tgcctacgct cattcccggc aagctgcact acagcaatct
     1741 tcgcagccac cgcaagatcc tcgttgtcga tggccagctc ggcttcaccg gagggatgaa
     1801 catccgccag gggcacctct gcgagccggg ctatccgtac ccggtcaagg acctgcattt
     1861 tcgcgtcgag gggcccgtcg tcgctcacct tcgcgacgcc tttgccgtcg attgggaatt
     1921 caccactggc gagcacctcg ggggcggcgc gtggttccct acgatcgctc ccgccggcca
     1981 ttcgctctgc cgcggcatcc ccgacgggcc ggacctcgac ttcgacaaga ttctgctgac
     2041 gatccacggc gccatcggtg tcgcccagcg ctcggtcgat atcatcacgc cctatttcct
     2101 gcctgagcag ccgctgatca ccgcgctcaa cgtcgccgcc atgcggggcg tggccgtcaa
     2161 catctacctg ccggagaaga acaatcttcg gctggtgcag tgggcctgcg cggcccagct
     2221 ctggcaggtg ctgcagcggg gctgccgcgt ctggcttacg gctccgccct tcgaccacac
     2281 caagctgatg atcgtcgatg gggcctgggc gatgatcggc tcgtcgaact gggacccacg
     2341 cagcctgcgg ctgaatttcg agttcaatgt cgagtgttac gaccgcgatt ttgccgccca
     2401 gctgagcgcc ttagtggccg ccaagcgggc ctccagccgc gagatcacgc tcgccgacgt
     2461 ggatggccga ccgctctggc aacgcctccg cgacggcgtc tgccgtctga tgctgccgta
     2521 cttgtaggtc ctcgactccc gacccctggc ccccgacccc cggcccctta tgatgggcac
     2581 gtggcaaaga accagtacga cgtcgtcgtg attggcaccg gccccggagg cgaaggggcc
     2641 gcgatgcaag cggtgaaaca cggcttgaag gtggccgtcg tcgagcggtt tgccaagatc
     2701 ggcggcggct gcacgcactg gggcacgatc cccagcaagg ccctgcgatt tgcgatcttc
     2761 cagatgctgg agatcaacag cagccgggtg tttcgcgagg cgggaatctc ggccgacgtg
     2821 agctttccgg acctccgcaa gaccgccgag agcgtcatcc gccagcagga ggacatgcgc
     2881 cgcggcttct acgagcggaa caacgtgccg atctactcgg ggcatgcccg cttcgtcgat
     2941 ccgcacacgc tggaagtcgg cgaggccaag ggggcgcagg agcgggtcac ggccgacaac
     3001 ttggtgatcg ccgtcggcag ccggccgtac cgcccgccag atattgattt ttcccatccg
     3061 cggattttcg acagcgatac gatcctcaac ctgggcttca cgccctggtc gatcactgtc
     3121 tacggggcgg gcgtcgtggg ctgcgagtac gcctcgatgt tccgcaacct gggctgcaag
     3181 ctgaacctgg tcaacacgcg ggcgcagctt ctggagtttc tcgacgacga gatttgcgac
     3241 gcgctgagct atcacttgcg cgagaaggga gcgctgatcc ggcacggcga acaatatgag
     3301 aagatcgagg gcaccgacga cggcgtgatt ctctcgctga agagcaacaa gcagctcaaa
     3361 acggacatcc tgctgtgggc gaacggccgc acgggcaaca ccgacaacct gggcctggaa
     3421 acgatcggca tccagcctga cagccgcggg cagctaaagg tcgatgatcg ctatgctacc
     3481 agcgtgccgc acatctacgc ggtgggcgac gtgattggct acccctcgct ggccagcgcg
     3541 gcgtacgtgc aggggcgggc cgccgcgctg cacattgccg gcgacgactg cgcccggctc
     3601 ctggccaacg acattccgac gggcatttac accagccccg aaatcagctc cgttggcaag
     3661 accgagcggg agctgaccgc cgcgggcgtg ccctatgaag tcggccacgc tcaattcaag
     3721 agccttgccc gggcccagat caccggccag accgtcggga tgctcaagat cctcttccac
     3781 cgcgagtcgc gggccgtcct cggcgtccac tgcttcggcg ctaacgccag cgagatcatc
     3841 cacatcggcc aggcgatcct cgcccagccg ccgccgaaca atacgctgat gtatttcatc
     3901 aacacgacct tcaactatcc gaccatggcc gaagcctacc gcgtcgccgc ccttaacggc
     3961 tacaaccggc tgttttgaaa tgtctgtgac tccgcccatc ggccgcctcg ctccgtcgcc
     4021 gacaggggcg cagcatgtgg gcaatgcgcg gacgtatttg ttggcgtggt tgtcgatccg
     4081 ctcgcggggc gggcgggtga ttctgcggat ggaggatatc gactcgccgc gggtcaagcg
     4141 cggagcggcg gagcaggccc tggcggattt gaagtggctg ggactcgatt gggacgaagg
     4201 accggacatc ggcgggccgc atgcgcccta tttgcaaact cagcggctgt cgctctatca
     4261 ggaggcgcta gagaaactgc aagccgccga gcgggtctat ccctgcacct gcacgcggtc
     4321 cgatgtggcc gctgcggcca gcgcgccgca cgccgggcag gaagggccgt tctatccggg
     4381 aacctgcgca ttccgcaccg ttgccgatgc cgcaacacta acccaaaacg caggcgaggg
     4441 acttcctgat ttcgcgtggc gattgcgaac gaccgatgcc gcgcgtcatt ttgttgatct
     4501 ggcggctgga tttcaatcgt gcaacgtcgc cgccgagctg ggcgacttcg tgatcgccaa
     4561 agccgacggc acgcccgcgt atcagctggc cgtggtggtc gatgaccacg cgatgggtat
     4621 caccgaggtc ctccgcggcg acgatctgtt gcccagcgcc ttccgccagc ttgagctgta
     4681 cgacttcttc gactggactc cgccgcaatt cgcccacgtg cctttggtag ttggtccgga
     4741 cggccgccga ctggccaagc gccacggcga cacgcggctg gccacgctcc gcgccgcggg
     4801 cgtcgccccg gagaggctag tcggcatgct ggctcactcc tgcggcctca tcgaccggcc
     4861 ggaaagcatt ccagcccgag acttgctgcc gagtttcagt ttgactcggc tgccgcagga
     4921 gccgtttgtg tttgcgggcc tttagcgtcc ttctcggtcc atgccaagag cagcggcagg
     4981 acgatcaggt tgccgagtgc cccgccgaac atggccaggc acatcagcgc gccgaaatag
     5041 atcgtgggca cgaagtcgct ggtgattagc acgccgaagc cgacgatcag ggcgatcgtg
     5101 gagaagatca tcgccagtcc gacgctctgc tggcaaagcg tgagcgcctc gtggacgctc
     5161 ttgccctcgc ggcggaagtg gcggaacgac gagaagtagt ggatcgagct gtccaccgac
     5221 atgcccatcg agaccgcggc gatcatcgcc gcccccatat tcatcttcag acccacccag
     5281 cccaaaaggc ccatcacgac ataaatcggg acggcgttgg gtatcatcgc gatcagagcg
     5341 tatttccagc tgcggtagcc gatgatgacc atgatgatga tcgccagcga cgagaccccg
     5401 aacgtcaccc attgatcgcg gagcatgctc tcgatcagat tcgtgagcag gacgaagaag
     5461 cccgtcactt ccgcgcccgg cgagtcgtcg gccagtggaa actcctcgcg ggccacacgg
     5521 gtgacctcgt tgatcagcca ccgcttttgc tccgccggtt gccgctcggg ggcccgcagc
     5581 atgatccgca gccgcgcctg gccgttctcg tcgagctttt gtgccaggag tgcggcggcg
     5641 aaagtcggca tcgcggcggc catgccctga tagcggagct ccggcgacag catcgccagc
     5701 gcccggtcgg cctcgatcgc gtcgatggcg tcaacgatgc tgatgacctt gcttagtgcc
     5761 ggcccaggcg ggccggccgg cgactggatg gcccgcaggc gttcttccag cttccggacc
     5821 cgggacagat agtctcgctc cagcgcaggc ggggcgggga tcactacatc ccagacgccc
     5881 gcgccgccca gattcgtctc gacgaagtcg taggccttca cgatccggct gccgcggcgg
     5941 aaattgtgcg tgaagtcggt ttcgacttct aagcgcactg cgcccaggga tgcgaccagc
     6001 gcgatgaacg cggtcacgaa ggccacggtc tttggccggg cttgcacggc atcgaccgag
     6061 cgcatcagcc actggccgag ctgcacttcc ccccagccat gttccttctt gccgtcgcgg
     6121 gcaaaggcca gcgcgagggc cggaaacatc aggcacatcg cgggcagcac gaggaacgag
     6181 ccgacgacca tcatcgtgcc gaaatcgcta acgggcttga ccgacgccca ccacagcgag
     6241 ccgaacccgg agacgtcggt cagcacggcg gcgaagatcg gtccggccag gagcactccc
     6301 gccttgagca tggccgctcg ctgagtcaga ccttgcgtcc gcagctcgcg gacttcgacg
     6361 atcaggtgca tcacgccggc cacgccgacc accgtgacaa tggcggtcaa catcgagctg
     6421 accatcgaga gctgcaggcc gctgacgacg agcgccgccc gcgtaacgat cagcgtccat
     6481 tgcacgacga cgatcggaat catcagccac cgcaggtgcc gaaagagcac caggatcgtc
     6541 agccctagca gcagcgtcgt ccaggtgccc agcttacggc cgtccttttc gagcagggcg
     6601 aagccgtcgc tgaccatgac cggttcgcct gccagcacgc cgggcgtcag gcctgcgggc
     6661 aagtcggtga tgattgcctc cagcgcctcg atcgtcgcgg cccgcgggtc ttcgtcgccg
     6721 gcggcggctg cgggtcttac cgggtcgagc atgcacacga ctgcggcgat cttgccgtcc
     6781 gccgaatgcg tgtagccgct gaacagctcg cgaaagcgct cggccagcgg gcttttgggg
     6841 ttgagaatgg gcggcccctt ccattcgtcg ggccgtcccg gaaagaagcg cctgaagccg
     6901 cggccgatct tgccgccctg caattgctcc aacaggccgt tcacttcggc gaggctgagc
     6961 acgtccttga cgccgggtac cgtcttcatt cgctggctga tgctcgccaa ccgctcgatg
     7021 ccttggcctt cgccggccag aagttgctcg tcgcgataga cagccacgac gatctcgttg
     7081 ccgccaaagc ggtccttcag ccgctggtag ggcggcaaga gcgggtcgtc cggcgcgaac
     7141 atcttctcga tgctgcggtc gaactgcacc tgattggaag gaccaaacgc cactgccgct
     7201 agcacgctcg ccacgaggag cagcggccag cgggcggcaa tgagcagttc gatgaaacgc
     7261 tgcatggggg atcaaggtaa cagggatcgg cgagcgtaac acaggggaca ccacatcttt
     7321 agcagtattg ccgcaggccc gcagacccgc ccccattcgc gagagccggc aatgccggag
     7381 agctttcctg tccggcgcgg agcgaccttt tccaacgtcg cgacactcca gacgctggca
     7441 atccgatatc actgctacca cgcaaaaacc tcccttgggc ggcggcgtgc gcgctggcgc
     7501 gggctagctg gcagttggcg gctgtccgct tcgttggcgc agatcgtttc tacccggatg
     7561 cctctttggc tgcggacccc ccccccggac cagcagccga ccaggaaagc gctgatgatt
     7621 cgcacgcttc tttccttttg cctcttgttt ggcgggctgg tcgcggtgag caagtggctc
     7681 cacgacacga cccgcccggc ggcgggcgac gcaatgccgg ccaactcgag tgccgagccg
     7741 gccccggcgc cgcctgcggc catgaagccg tcccgcttgc cttcgagcgg cgaggcacag
     7801 cccgccaagg tgcgtttggt cgcgtttgaa gacgacgagc cggatgcgcc gaagaagaac
     7861 aacccggttg gcagtacccc gaagaccgag ccgaccgaag ggga
//
LOCUS       DASRJB010000901         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4203577, whole genome shotgun sequence.
ACCESSION   DASRJB010000901 DASRJB010000000
VERSION     DASRJB010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4203577"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFB80_23755"
     CDS             <1..856
                     /locus_tag="VFB80_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN36874.1"
                     /translation="PGGMPIKDSENWQSAFLPGAYQGTFIDSKHERLDQLIENIAHPH
                     ATTEMQRRQLDLLARLNAAHKEGRLDDRLDARIGAFELAFRMQTEAAEAFDLSREPQA
                     TRDLYGSGVHGRQTLIARRLLERGVRYVQLWHGAGQPWDNHDNIEANHKKLAAEIDGP
                     IAALLTDLKRLGMFDDTLVIWGGEFGRTPSVELGEGGKSKLGRDHNHYGFSVWLAGGG
                     VKGGTTYGSTDEFGFKAAENPTSVHDLHATILHLLGFDHERLTYRYAGRDFRLTDVSG
                     HVLSEILA"
     gene            970..2928
                     /locus_tag="VFB80_23760"
     CDS             970..2928
                     /locus_tag="VFB80_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN36875.1"
                     /translation="MTRVAALLALLVPLLANGQQRAENRQSAAHTNRLAKETSPYLLQ
                     HAQNPVNWYPWGEEALAKAKQEGKLIFLSIGYSSCHWCHVMERESFHDEEIAKVLNEH
                     FVCIKVDREERPDVDSVYMTSLHMINLLAERGRGGGWPLSMFLTDEAEPFFGGTYFPA
                     RDGDRHEGSTGFLTICRKLQEYWKQSPAKVREDAKTIVKFTKAELERRRGALTVALDE
                     ALLVAAQEALREQFDPKYGGFGYTPDGRRPKFPEPSNLVLLLDRVRRHSDAQARQMLV
                     KTLDQMAMGGIRDHLGGGFHRYSVDRYWAIPHFEKMLYDNGQLASVYAQAHELTGNEN
                     YRRVARELCEFVLREMTHEGGAFYAALDADSEGEEGKFYRWEKAEVQKTLSAEEFALF
                     AKVYGLDGEPNFEEKYFAPQLSKPLAESAAEMKLTEAALEARLAPLRKKLFDVRAPRV
                     RPRTDTKVLTSDSGLMIGGLADAGRILKESRYVAAAEKAAEFVLTNLRTADGRLLRTF
                     AGGEAKLNAYLNDYAYLADGLLRLHHATGNARWLDEAAAVTAKQIELFSDARAGGFFF
                     TSSDHEALLARCKDLADNAIPAGNSVAAHNLIALAVLKNKPEYLPLASQAIATAVVAT
                     QSSPGAAPWMMTAIPALADAKRKLGSGK"
     gene            2935..3960
                     /locus_tag="VFB80_23765"
     CDS             2935..3960
                     /locus_tag="VFB80_23765"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN36876.1"
                     /translation="MAGEPQRTFHLVAEHDGLTLAAALKRLAPKLSWSQARKLLAGRR
                     VQVNGNLCLDEERKVKAGDVVRLSSHALAPPATADDVKLVYVDEHLVVVQKPAGVTTL
                     RHREETDLPQRRKQLQPTLDELLPRLLARHLGLRYTTPTRQGGERHRGRSLQRDVRLA
                     LHPVHRLDRDTSGLLVFARTRGAEEKLIHMFARHQVQRAYVAVVQGRIDRERTIESWL
                     VRDRGDGLRGSSPLGEAAEGAQRAVTHVRPMEQLANCSVVECRLETGRTHQIRIHLSE
                     AGHPLCGEKTYTHAPGEPARADASGAPRQALHSAELALSHPISGQALRFRMALPADLK
                     QWLLRMR"
     gene            complement(3964..5097)
                     /locus_tag="VFB80_23770"
     CDS             complement(3964..5097)
                     /locus_tag="VFB80_23770"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN36877.1"
                     /translation="MRIAAIETYPIRIPLKVAYRMISALGRHEESHFVLVRLRTDDGL
                     EGAGEASLTVRWSGETPWGAVALIDRLLAPLLLGMELDPAQPLESIAAIDRRMDAAVQ
                     HNWFSKSAIEMACWDVLGKAAGRPVYELLGGACRPLAIRSRFSMGAYDVPRARARAAE
                     LVEQGFDTVKVKVGGRAEDDIARVRAVREVIGSERKLVIDANCGWDADTAIRAVRELA
                     DARVDLLEQPTGDGDYAAIARVRRECGVPVMADDMCFNLVHARELIRNQACDLISVYP
                     GKNGGIRKSRAIVEFAEQHNVGCTIGSNLEFDVATAAMGHLIVATPNLRVEEWPGDIH
                     GPAYYEVRIVKEPLSIAGPVTTLAARPGLGVEVDWGLVRSLHA"
     gene            complement(5104..6693)
                     /locus_tag="VFB80_23775"
     CDS             complement(5104..6693)
                     /locus_tag="VFB80_23775"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HZN36878.1"
                     /translation="MLLGYVSNERYLALSDVEVEILGPHGSLAVRSRASGSLHADIES
                     GRYEVVLAKEGYGPKRVIADLTPDRLHHFRLLKDDLLGYAWPKWVQAGQASEFRVHSD
                     AAYKLELWRYGWRKELVRPIGWFDEHGPRATVQITPDGDYSQTGVTWNKYGYTSPHHK
                     QFITAPQPSGLYYFHAASEAGRFFSFPWIVAPDRPRAKIAVLASNITWNAYNSFGGRS
                     NYIHPDRLPPTPTVNARLELKRYTDAEHVNYDTTDYAPLSFDRPESINHVPEHVQATD
                     PIAGRAACHLAPAEWRLLAWLERERLEYDYFAETQLHFGQLDLDAYRVLVISTHPEYW
                     SREMFDRVKRWVFERGGRLVYLGGNGLNCDVEFLDEQTCIYRNDNERRLRGNDAPYES
                     RFHLRNESEASLLGVVYDARGIMTSAPYRVVDPGHWVFEGTGPAEGSIFGERSLHERV
                     PGGASGHETDKISKSSPPGTRVLAKGLNPDGGGADLSIIESAGQGAVFSAGSITWVSS
                     ILVDDAVSRITRNVLTRFASK"
     gene            6880..>7079
                     /locus_tag="VFB80_23780"
     CDS             6880..>7079
                     /locus_tag="VFB80_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36879.1"
                     /translation="MRRPTFPGRLLRGFTLVELLVVIAIIGVLVALLLPAVQSAREAS
                     RRTKCMNNLKQQALAAHNHEDT"
BASE COUNT         1254 a         2349 c         2314 g         1162 t
ORIGIN      
        1 tcccggcggc atgccgatca aggattccga gaactggcag tcggcgtttc tgcccggggc
       61 ctaccagggc acgttcatcg actccaaaca cgagcggctg gaccagctga tcgagaacat
      121 cgcccatccg cacgccacga ccgagatgca gcggcggcag ctcgacctgc tagcgcggct
      181 aaacgcagcc cacaaagagg gccggctcga cgatcgcctg gatgcccgga tcggggcctt
      241 cgagctggcg tttcgcatgc agaccgaagc cgccgaggcg ttcgacttat cgcgcgagcc
      301 gcaggccacg cgcgacctgt acggcagcgg cgtgcacggg cggcagacgc tgatcgcccg
      361 ccggctgctg gagcgcggcg tgcggtacgt gcagctctgg cacggcgcgg ggcagccctg
      421 ggataaccac gacaacatcg aagccaacca caagaagctc gccgcggaaa tcgacgggcc
      481 gatcgcggct ctgctgacgg acctgaagcg gctgggcatg ttcgacgaca cgctcgtgat
      541 ctggggcggc gaattcggcc gcacgccgtc ggtcgagctg ggcgagggcg gcaaatccaa
      601 gctcggccga gaccacaacc actatggctt ctcagtctgg ctggccggcg gcggcgtgaa
      661 gggcggcacg acctacggct cgaccgacga gttcggcttc aaggccgccg agaatccgac
      721 cagcgtgcac gacttgcacg ccacgatcct gcacctgctc ggcttcgatc acgagcggct
      781 gacctatcgc tacgccggcc gcgacttccg cctcaccgac gtcagcggtc atgtcctcag
      841 tgagatctta gcctgacgcc tgcctcgctt tgtgccgcga ccaggccggc acacgctgac
      901 tgggggctgg ggctaagggt gtgccggctt gctacaatcg gccgtatggt taataccaac
      961 agccgccgaa tgacccgcgt cgccgcgctt ctcgcactgc tcgttccact gctggccaac
     1021 gggcaacagc gggccgagaa tcgccaatcg gccgcgcata ccaaccgcct ggcgaaagag
     1081 accagcccgt atctgctcca gcacgcgcag aatccggtca attggtatcc atggggcgag
     1141 gaggccctgg cgaaggccaa gcaggaaggc aagctgatct tcctgtcgat cggctattcg
     1201 agctgccact ggtgccacgt gatggagcgg gagagctttc acgacgagga aattgccaag
     1261 gtcctcaacg agcatttcgt gtgcatcaag gtggaccgcg aggagcggcc ggacgtcgat
     1321 tccgtctata tgacttcgct gcacatgatt aacctgctgg ccgagcgggg ccgcggcggc
     1381 ggctggccgc tgtcgatgtt cctgactgac gaggcggagc cgttcttcgg cggcacttat
     1441 ttcccggccc gcgacggcga ccggcacgaa ggctcaacgg gctttctgac gatttgccgc
     1501 aaactgcagg agtattggaa gcagtcgcca gcgaaggtcc gtgaggacgc caagacgatc
     1561 gttaagttta cgaaggccga attggagcgc cgccgcgggg cgctgacagt cgcgctcgac
     1621 gaggcgctgc tcgtcgcggc ccaggaggcc ctccgcgagc agttcgaccc caagtacggc
     1681 ggcttcggct acacgcccga cggccggcgg ccgaaattcc ccgagccgag caacctggtg
     1741 ctgctgctcg accgcgtgcg gcggcacagc gacgcgcaag cgcggcagat gctcgtgaag
     1801 acgctcgacc agatggcgat gggcggcatc cgcgaccact tgggcggcgg ctttcaccgc
     1861 tatagcgtcg atcgctattg ggcgatcccg catttcgaaa agatgcttta cgacaacgga
     1921 cagctcgcga gcgtgtatgc ccaggcgcat gagctgacgg gtaacgagaa ttaccgccgc
     1981 gtcgccagag agctgtgcga gtttgtgctc cgcgaaatga cgcacgaagg cggcgcgttc
     2041 tatgccgccc tcgacgccga ctccgaaggc gaagaaggga agttctatcg ctgggaaaag
     2101 gccgaagtgc aaaagacgct ctcggccgaa gagttcgcgc ttttcgctaa agtctacggc
     2161 ctggacggcg agccgaactt tgaggagaaa tacttcgcgc cgcagctctc caagccgctg
     2221 gcggagtcgg cagcggagat gaagctgacc gaagcggcgc tggaggccag gctcgcaccg
     2281 ctgcgcaaga aactgttcga cgtccgcgcg ccgcgcgtcc gcccgcggac agataccaag
     2341 gttctcactt ccgacagcgg cctgatgatc ggcgggctgg ccgatgccgg ccgcatcctg
     2401 aaggagtcgc ggtacgttgc cgccgccgag aaagccgctg aattcgtcct cacgaatctc
     2461 cgcaccgcgg acggacggct gctgcggacc ttcgccggcg gcgaggcgaa gctgaatgcc
     2521 tatctcaacg actatgccta cctggccgac ggcctgctgc ggctgcacca cgccaccggc
     2581 aacgcccgct ggctcgacga ggccgcggcg gtcacggcaa aacagatcga gctgtttagc
     2641 gacgcgcggg ccggcgggtt cttcttcacc tcgagcgatc acgaggcgct cttggctcgc
     2701 tgcaaggact tggccgataa cgcgattccg gcgggcaact cggtcgccgc gcacaaccta
     2761 atagctctgg cggtactgaa gaacaaaccc gagtacctgc cgctggcttc tcaggcgatt
     2821 gccaccgccg tggttgccac gcagagctcg cccggcgccg cgccgtggat gatgacggcc
     2881 attcccgcgc tggccgatgc gaagcgcaag ctcggctccg gaaaatgact tcgcatggcc
     2941 ggcgaaccgc agcgcacgtt tcatctggtg gccgagcacg acggtctgac gctcgcggcg
     3001 gccctcaagc ggctcgcgcc caagctttcc tggtcgcagg cccgcaagct cctcgctggc
     3061 cgccgcgtgc aggtcaacgg caacctctgt ctcgacgaag agcggaaagt caaggccggc
     3121 gatgtcgtgc gcttaagcag ccacgcgctg gctccgcccg cgacggccga cgacgtcaag
     3181 ctggtctatg tcgatgagca cctggtggtg gtccaaaagc cggccggcgt cacgacgctg
     3241 cggcaccgcg aggaaaccga cctgccgcag cgccgcaagc agctccagcc gacgctcgac
     3301 gaattgctgc cgcggctgct cgcccggcac ttggggctgc gctacaccac cccgacgcgg
     3361 cagggagggg agcgccacag agggcgttcc ctacagcggg atgtgcggct tgcactccat
     3421 cccgtacatc gcctcgaccg cgacacgagc ggcctgttgg tcttcgcccg cacgcgcgga
     3481 gcggaggaga agttgatcca catgttcgcc cgccaccagg tccagcgggc ctacgtcgca
     3541 gtcgtgcagg ggcggatcga tcgggagcgg acgatcgagt cgtggctggt ccgcgaccgg
     3601 ggcgacggcc tgcgcggcag ctcgccgctg ggcgaggcgg ccgaaggggc acagcgggcg
     3661 gtcacgcacg tccggccgat ggagcagctc gccaactgct ccgtcgtcga atgccgcctg
     3721 gaaacgggac gcacgcatca gattcgcatc catttgtccg aggccggcca tccgctttgc
     3781 ggcgagaaaa cctataccca cgcgccgggc gagccggcgc gagccgacgc cagcggtgcc
     3841 ccgcggcagg cactgcattc tgctgaattg gccctgtccc acccgatcag cggccaggcg
     3901 ctgcgcttcc gcatggcgct gcccgccgat ctcaagcagt ggctgctgcg aatgcggtag
     3961 ccgctacgcg tggagcgagc ggaccagccc ccaatcgact tcgacgccga ggcccggtcg
     4021 cgcagcgagc gtcgtgacgg gcccggcgat cgacagcggc tccttcacga tgcggacttc
     4081 gtagtaagcc gggccatgga tgtcgccggg ccactcttcc acccgcaggt tgggcgtcgc
     4141 cacgatcagg tggcccatcg ccgcagtggc gacgtcgaat tccagattgg agccgatcgt
     4201 gcagccaacg ttgtgctgct cggcgaactc gacgatcgcc cgcgacttgc gaatgccgcc
     4261 gtttttgccg ggatacacgc tgatcaagtc gcaggcctgg ttgcggatca gctcgcgggc
     4321 gtggaccagg ttaaagcaca tgtcgtcggc catgaccggc acgccgcatt cgcgccgcac
     4381 gcgggcgatc gccgcataat cgccgtcacc cgtcggctgt tccagcaaat cgacgcgggc
     4441 gtccgcgagc tcgcgtaccg cgcggatcgc agtatcagcg tcccagccgc aattggcgtc
     4501 gatcacgagc tttcgctcgc tgccgatcac ttcgcgcacg gcgcggacgc gggcaatgtc
     4561 gtcctcggca cggccgccga ctttgacctt caccgtgtcg aatccctgct cgaccagttc
     4621 cgccgcgcga gcgcgggcgc gcggcacgtc ataggccccc atcgagaaac ggctgcgaat
     4681 cgccagcggc cggcacgcgc cgccgagcaa ctcgtacacg ggccggccag ccgccttgcc
     4741 cagcacgtcc cagcaggcca tctcgatggc gctcttggag aaccagttat gctgcaccgc
     4801 cgcgtccatc cggcgatcga tcgctgcgat gctctccagc ggctgcgcgg gatcgagctc
     4861 catgcccagc agcagcggcg cgagcaggcg atcgatcagc gcaaccgcgc cccagggcgt
     4921 ctcaccgctc cagcggaccg tcagcgaagc ctcgccggcc ccttccaggc cgtcgtcggt
     4981 acgcagccgc accaggacga aatgcgattc ctcgtgtcgg ccaagcgccg agatcatccg
     5041 atacgccacc ttcagcggaa tgcggatggg gtaggtttca atggcggcga tacgcatagt
     5101 gggctatttc gaagcaaacc tcgtaagcac gttgcgtgta atgcggctga ctgcgtcatc
     5161 gaccaggatc gagctgaccc aggtgatgga gccggcggag aagacggcgc cttggccggc
     5221 ggattcgatg atcgacaagt ccgcgccgcc accgtccgga ttgagccctt tggcaagaac
     5281 ccgcgtgccc ggtggcgaag acttggaaat cttgtcggtc tcgtgccccg acgcgccacc
     5341 ggggacgcgc tcgtgcagcg atcgttcgcc gaagatgctg ccctcagcag ggcccgtccc
     5401 ctcgaatacc cagtggccag gatcgacaac ccggtaggga gcggaagtca tgatcccgcg
     5461 ggcatcgtag acgacgccca aaagcgacgc ctccgattcg ttccgcagat ggaagcggct
     5521 ttcgtacggc gcgtcgtttc cccgcagccg gcgctcgttg tcgttgcggt aaatgcaggt
     5581 ctgctcgtcc aggaattcca cgtcgcaatt cagaccgttg ccgcccagat agaccagccg
     5641 gcccccgcgc tcgaagaccc agcgcttcac gcggtcaaac atctcgcgcg accagtactc
     5701 cggatgtgtg ctgatgacga gcacgcgata ggcgtccaag tcgagctgcc cgaagtgaag
     5761 ctgcgtttcc gcgaagtagt cgtactccag ccgctcgcgc tccagccagg ccagcagccg
     5821 ccattcggcc ggcgcgaggt ggcaggctgc ccgcccggcg atcgggtcgg tcgcctgcac
     5881 gtgctctggc acgtggttga tcgactctgg ccggtcgaac gacagcggag cgtagtcggt
     5941 cgtgtcgtag ttcacgtgct cggcgtcggt gtaccgcttc agctccaggc gggcgttgac
     6001 ggtcggcgtc ggcggcagcc ggtcggggtg gatgtagttg ctccggccgc caaagctgtt
     6061 gtaggcgttc caggtgatgt tcgacgccaa caccgcgatt ttcgcccgcg gccggtccgg
     6121 cgcgacgatc cagggaaacg agaaaaagcg gccggcctcg ctcgcggcat gaaagtaata
     6181 cagccccgac ggctgcggcg cggtgatgaa ctgtttgtgg tgcgggctcg tgtagccgta
     6241 cttgttccag gtcacgccgg tctgtgaata gtcgccatcg ggcgtgatct gcaccgtcgc
     6301 ccgcggcccg tgctcgtcga accagccgat cggtcggacc agctcttttc gccagccgta
     6361 gcgccacaat tccagtttgt aggccgcgtc gctgtgcacg cgaaactcgc tggcctgccc
     6421 ggcctggacc cacttgggcc aggcgtagcc gagcaggtcg tctttcaaga gtcgaaaatg
     6481 gtgcagccgg tcgggagtca aatcggcgat gactcgcttg gggccgtatc cctccttggc
     6541 cagcacgact tcataacggc cggactcgat gtccgcgtga agggagccgc tggcccgcga
     6601 gcgcacagcc agcgatccgt gcgggccgag aatctcgact tcgacgtcgc tcagggccag
     6661 gtaacgttcg ttgctgacgt atccgagcag catggtgaag ctgtttcgat tgttagggcg
     6721 ccgcgaagca accgcattct acaaactgca tcaattccgg gtacgacggt gcgtttctct
     6781 ttcgcggcag tttcgaaccg gtctataatc ggcagcggaa tcagagggtg acctgagcag
     6841 ccgttgcggt ctagctgcac cttttctcgg gggttttcca tgcgacgtcc aacgttccct
     6901 ggtcgtttgc tgcgcggctt cacgctggtg gagctgctgg tggtcatcgc catcatcggc
     6961 gtgctggtcg cgctgctgtt gccggccgtg caatcggccc gcgaggcatc gcggcgaacg
     7021 aagtgcatga acaacctcaa gcaacaggcg ctcgccgccc acaaccacga ggacaccta
//
LOCUS       DASRJB010000902        10719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1655519, whole genome shotgun sequence.
ACCESSION   DASRJB010000902 DASRJB010000000
VERSION     DASRJB010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655519"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFB80_23785"
     CDS             <1..534
                     /locus_tag="VFB80_23785"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZN36880.1"
                     /translation="KDRLSEQAVARLIELPDYQRARTALWYIDCRSELRTKHALPAAL
                     ASGKRIVVPYCTVDEAGANKLGLWWLQSMDELIVGKWKILEPPRERWGEPGKEIPPEL
                     IDFAIIPGVGFSRQGGRMGNGQGYYDRLLPQLRRDCPLVALCYECQLFDDLIVGPHDV
                     FMDKVVTERAVNPGRGR"
     gene            542..1471
                     /gene="fhcD"
                     /locus_tag="VFB80_23790"
     CDS             542..1471
                     /gene="fhcD"
                     /locus_tag="VFB80_23790"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: protein motif:HMM:NF002554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HZN36881.1"
                     /translation="MPAIIDDTYAEAFRSIYAEFLITAANRRWVEHAVNAATGNASST
                     ILCDCEAGLDRYVGKGGDESFATPDGRPGAIVQLHVPRFRKDRVAALERAALVRISQN
                     VLTCPTAACFNLIDSESYFQLGRKVAYFGDGFQQRIERHGRQLWWIPTLGGEFILDRR
                     LGYADGLMGGNLWFFGESTDSALAAAEAGVAAVQNCPGVVMPFPGGIAGSGSKAGSKY
                     KFLFASTYEKYCPLLRDNPAAKCELPPGVNSVMEIIINGQSLAAIVRATQAAIAAAVN
                     TPGLLRISAGNYGGRLGKSFIYLHPDKQPAEAK"
     gene            1582..2073
                     /locus_tag="VFB80_23795"
     CDS             1582..2073
                     /locus_tag="VFB80_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN36882.1"
                     /translation="MGLKQRLQRNLRNMRQMSQRLLADFKTPEQWVFQVHPQCNHALW
                     FAGHMATSDNFFLSLVAPEKARQLPDYQSRFGMGSQPTNDPASYPPPASVVETMNERR
                     QTLLSVLDAMSEEDLAKKTAAGAPEFLSDVASVFEMAVWHEGQHNGQLTVARRVLGHK
                     PLF"
     gene            complement(2143..2805)
                     /locus_tag="VFB80_23800"
     CDS             complement(2143..2805)
                     /locus_tag="VFB80_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZN36883.1"
                     /translation="MASLTIENYVKAIYQISAGAGGGATSTGKLAEALGVSPGTVTSM
                     LKTLGESGLAEYVPYEGARLTDAGRILALRVLRRHRLIELFLVRTLDLSWDEVHEEAE
                     NMEHAVSDLLIDRIDAFLGHPATDPHGDPIPKADGTVAGTSGRRLSQLAVGQRFRVVR
                     VLEQTPEFLRYLTETGLKLDATGTVAANRSESGTITVQVAGQSTTLAHDVADRLLVTD
                     KG"
     gene            2922..3866
                     /gene="mch"
                     /locus_tag="VFB80_23805"
     CDS             2922..3866
                     /gene="mch"
                     /locus_tag="VFB80_23805"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HZN36884.1"
                     /translation="MTTSLNERAWHLADQFAADSEHLRASVSQVAGARVIDCGVQASG
                     GLEAGRRLAEICLAGLGEVTIEDGTLETGPAVTVRTDHPVLACMASQYAGWQITGEKF
                     FGMGSGPMRAAACREPLFEHLGYREQAARVVGVLETSKLPTEEVCRKIAEQCGVAPEK
                     LTLLVARTASIAGTVQVVARSVETGLHKLHELGYDLKNIISGFGSAPLPPVAADDLVG
                     IGRTNDAILYGGDVTLWVRDDDARLAQIGPQIPSSASRDYGEPFAAIFQKAGRDFYKI
                     DPLLFSPAAVTLNNLATGKSHRFGQLRPDVLSRSYSEP"
     gene            3890..4783
                     /locus_tag="VFB80_23810"
     CDS             3890..4783
                     /locus_tag="VFB80_23810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HZN36885.1"
                     /translation="MRFAVLAAPESWYARDLTRAAAGDHEVVTLPFSAVSAQIDEQGR
                     TQVLCGGRDLADFDAVLVRTMPPGSLEQVVFRMDCLGRYEAASGVVINPARAVEAAVD
                     KYLTSAKLAASGLLTPRTICCQTADDALAAFEQLGGDVVLKPLFGSEGRGIARLNDEA
                     LAERAFKMLAQLGAVLYLQEFVPHEGCDVRLLVIGERILAMRRCNPLDWRTNISRGAA
                     AEPLVPDDALAEIARRAAAAVGAPVAGVDVLPGRDGRLYTLEVNAVPGWKGLAKAHGV
                     DVARLVLDFAASEVQSRTRRE"
     gene            4799..6520
                     /locus_tag="VFB80_23815"
     CDS             4799..6520
                     /locus_tag="VFB80_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HZN36886.1"
                     /translation="MQQSAPAETRLPTPDPRHPAIVNVGLRLSETARLNPNGVAVVVP
                     RGRDGGKRIYASLTFRELDDDSNLLADGLAAMGARPGTRLVLMVPPSIDFISLVFAMF
                     KAGVVSVLIDPGMGRRNLIRCLVESEPEGFIGVPLAQAVRCVLRGRFPNAKLNVTVGR
                     RWFWGGRTIAQLRARKLSADWRPAVTKADDPAAIIFTTGSTGPPKGTLYRHGNFDRQA
                     DEIRDFYRIQPGEIDLPGFPLFALFNCAMGVTTVIPDMDPTRPARVDPRNIVEAVTDW
                     KVTQAFGSPALWNVVGRYCEERQIVMPTLRRVMSAGAPVPPHVLQRMKAAIAPEGDVH
                     TPYGATEALPVASIAASEVLAETAAKSAAGAGTCVGRHFPGIDWKVIRITDGPLATLE
                     RAEELPSGEIGELIVRGPVVTTEYVTRTECNALHKIRDGQSFWHRMGDVGYLEHERFW
                     FCGRKSHRVITPRGTLFTIPCEAIFNQHPRVYRSALVGVGGPGQQRPVIIVEPWPEKR
                     PRSTSDAAQLIGELRELGQQHAHTAEIGDFMIRNALPVDIRHNAKIFREQLAIWAAWQ
                     LGTTAQA"
     gene            complement(6522..7649)
                     /gene="rsgA"
                     /locus_tag="VFB80_23820"
     CDS             complement(6522..7649)
                     /gene="rsgA"
                     /locus_tag="VFB80_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZN36887.1"
                     /translation="MGRKRKIRHEFRKQHQGRKRQGDLTRQVLGDENAEDRLVRSERL
                     TGKGDLTRKRTIVGVEADAETAGFAVLRDVDGTAIRGHVLSVHGLSSVVQADDGRQFR
                     CAVRGVLKDLSTDLRHVVVAGDIVWIRPEATGDSLIERIEPRARILSRTSKGRQQILV
                     ANVDQLVIVTSAAEPTLKPNLIDRFLVEAEQMRIEPILCINKIDLVDPADLQPFAGVY
                     GQMGYRVLLVSATTGQGIDQLRRLVAGKESVVAGQSGVGKSSLLNAIEPELALKVGEV
                     SRENQKGKHTTTVARLIPLAIGGFVVDTPGIRQFQLWDIVPEEVAGLYRDIRPYVSRC
                     RFPDCTHTHEADCAVKDAVADNRLDARRYESYCHVRAGDEE"
     gene            8644..9951
                     /locus_tag="VFB80_23825"
     CDS             8644..9951
                     /locus_tag="VFB80_23825"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN36888.1"
                     /translation="MLPILRQKNCTGCHQPAKKGGEYVMTDFAALLKGGESGSAAVVP
                     GDPKKSYLIDQITPGKDGKAEMPKELPPLTSSEIDTITKWIAQGAKDDTPASNRPQYD
                     AAHPPIYMAAPVLKSVEFSPDGTLLAVSGYHEVLLHKADGSALAGRLVGQSERIESAR
                     FSPDGKFLAVSGGSPGRFGELQIWDVEKRELKLSLMIGYDTLYGASWAPSGKMVAIGC
                     ADNTVRAFDAETGKQVLFNGAHSDWVLDTTFSVGSDHIISVSRDMSMKLIEVKTERFI
                     DNLTSITPGALKGGLISIDRHPTKDELLCGGSDGAPKIFKMVRTEARKIGDNANLLRE
                     FAELPGRVFGVAYNHDGSRIAAGSSSDGVGEVRVYNAMDGAVVWKVSVPEGGIFTVDF
                     NKDSTIVAAGGFDGDVRLYNATDGTLIKRFTPVEITGAAVAAN"
     gene            10025..>10719
                     /locus_tag="VFB80_23830"
     CDS             10025..>10719
                     /locus_tag="VFB80_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36889.1"
                     /translation="MLSLRGDEVPSEKLPAGLNVVALEARPATVELTHKFDYRQLLIT
                     GKLDSGETVDLTRMAQVSSPGTAVAVSKDGLVRAKADGTEEITFTFGGLSTKVAAKVS
                     GVAQPHVVSFVRDVQPALSRMGCNAGTCHGAREGKAGFKLSLRGYDALYDHRAFTDDI
                     GGRRFNRAAPDQSLMLLKATGSIPHVGGVRTNVGEPYYELVRDWINQGVKLDLKAPRV
                     TKIEVFPVNPVVPR"
BASE COUNT         1846 a         3487 c         3618 g         1768 t
ORIGIN      
        1 aaggaccggc tcagcgagca ggccgtggcc cggctgatcg aattgcccga ctaccagcgg
       61 gcacggactg cgctgtggta tatcgattgc cgctcggagc tgcggacgaa acacgctctg
      121 ccggcggcgc tggccagcgg caagcggatc gtcgtaccgt attgcaccgt cgatgaggcc
      181 ggtgcgaaca agctcggcct gtggtggctg caaagcatgg acgagctgat cgtcggcaag
      241 tggaagatcc tcgagccgcc gcgcgagcgg tggggcgagc ccggcaagga gattccgccg
      301 gagctgatcg acttcgcgat cattcccggc gtcggcttca gccgccaggg cgggcgaatg
      361 ggcaacggcc agggctacta cgatcggctg ctgccgcaac tgcgccgcga ctgcccgctg
      421 gtggcgctgt gctacgaatg ccagctgttc gacgacctga tcgtcggccc gcacgatgtg
      481 ttcatggaca aggtggtcac ggaaagggcg gtcaatccag gccgcgggcg gtaagcttct
      541 catgccggcc atcatcgacg acacctacgc cgaggccttt cgcagcatct acgccgagtt
      601 tttgatcacg gcggccaatc gccgctgggt ggagcacgcc gtgaatgcgg cgacgggcaa
      661 cgcttcgagc acgatcctct gcgactgcga ggccggcctg gatcgctacg tcggaaaggg
      721 cggcgacgaa tcgttcgcca cgcccgacgg ccggcccggc gcgatcgtgc agttgcacgt
      781 gccgcggttc cgaaaggacc gcgtggcagc gctggagcgg gcggcgctgg tccgcatcag
      841 ccagaacgtg ctcacctgcc cgacggcggc gtgcttcaac ctgatcgact cggagagcta
      901 tttccaactg ggccgcaagg tcgcctattt cggcgacggg tttcagcagc ggatcgagcg
      961 ccacgggcgc cagctgtggt ggattcccac gctcggcggc gagttcatcc tcgatcgccg
     1021 cctgggctac gccgacggcc tgatgggcgg caacctgtgg ttttttggag agagcaccga
     1081 ctcggcgctg gcagcagccg aagcgggcgt ggccgcagtg cagaactgtc cgggcgtcgt
     1141 gatgcctttt cccggcggca tcgccggcag cggctccaag gccggcagca aatacaaatt
     1201 tctgttcgcc agcacgtacg aaaagtactg cccgcttttg cgcgacaatc cggccgccaa
     1261 gtgcgaattg ccgccgggag tgaactcggt gatggagatc atcatcaacg gccagagcct
     1321 cgcggcgatc gtccgcgcca cgcaggccgc gattgccgcg gcggtgaata cgccgggttt
     1381 gctgcgaatt tcggccggca actacggcgg ccggctgggc aagagcttca tttatctcca
     1441 tcccgacaag cagccggcgg aggcgaagta gcggcaggct tgagtcgcca aagtgtccac
     1501 gggcccactt cccgcggccg gtcgaacggc ggctgccagg cgtgatggcg acatacaatg
     1561 catgcgaaac caggaacaac tatgggactg aaacaacgct tgcagcgaaa cctgcggaac
     1621 atgcggcaga tgagccagcg gctgctggcg gacttcaaga cgcccgagca gtgggtgttt
     1681 caggtgcacc cgcagtgcaa ccacgccctg tggttcgccg ggcacatggc gaccagcgac
     1741 aatttcttcc tgtcgctagt cgcgccggag aaggcccggc aactgcccga ctatcagagc
     1801 cgctttggca tgggatcgca gccgacgaac gacccggctt cgtatccgcc gcccgcgagc
     1861 gttgtggaaa cgatgaacga gcggcggcag acgctgctgt cggtgctcga cgcgatgagc
     1921 gaggaggacc tggccaagaa gacggccgcc ggagcgccgg agtttctgtc cgacgtggct
     1981 tcggtcttcg agatggccgt ctggcacgag ggccagcaca acggtcagct caccgtcgcc
     2041 cgccgcgtcc tgggtcacaa gccgctgttc taaaaagggc tagggccagg tgctagggct
     2101 ggggcagagt aagaacgaga tcccgagccc tagcccccag ccctagccct tgtcggtcac
     2161 gagcagccgg tcggcgacgt cgtgcgcgag cgtggtggac tggccggcga cctgaacggt
     2221 gatcgtgccg gattcgctgc ggtttgcggc gacggtgccg gtggcgtcga gcttgagtcc
     2281 cgtttcggtg aggtagcgga ggaattccgg cgtctgctcc agcacacgga cgacgcggaa
     2341 tcgctgcccg acggcgagct gcgacagccg ccggccgctg gtgcctgcga cggtgccgtc
     2401 ggctttggga attgggtcgc cgtgcggatc ggtggctggg tggcccaaaa aagcgtcgat
     2461 ccgatcgatc aacagatcgc tgaccgcatg ctccatattc tcggcttcct cgtgcacttc
     2521 gtcccagctc aggtcgagcg tgcgcacaag gaacagctcg atcaggcggt gccgccgcag
     2581 cacgcggagc gcgagaatcc gcccggcatc ggtcagccga gcgccttcgt aggggacgta
     2641 ctcggccagg ccgctttcgc cgagcgtctt gagcatgctg gtcacggtgc cgggcgagac
     2701 gccgagcgcc tcggccagct tgcccgtgga agtcgcgccg ccgcccgcgc ccgcggagat
     2761 ctggtagatc gccttgacgt agttctcgat ggtcaggctg gccacgcggc tgctccggaa
     2821 tgggcctgtc attctagccc ccgcgccgtc ggcgtgaatc gcccgggctg ttaccatcaa
     2881 agacttggat tgcgtcccat ccgtcccaca agccccatcg cgtgaccacc agcctcaacg
     2941 agcgcgcctg gcatctggcg gatcaattcg ccgcggacag cgaacacttg cgcgcgtccg
     3001 tctcgcaagt cgccggcgcg cgggtgatcg attgcggagt gcaggcctca ggcggattgg
     3061 aagcgggacg gcggctggcc gaaatctgcc tcgccggatt gggcgaggtg accatcgaag
     3121 atggaacgct cgaaaccgga ccagcggtca cagtgcggac cgaccatccc gtgctggcct
     3181 gcatggcgtc gcaatacgcc ggctggcaaa tcacgggcga aaagttcttc ggcatgggca
     3241 gcggtccgat gcgggcggcg gcgtgccgcg agccgctgtt cgagcacctg ggctaccgcg
     3301 agcaggccgc ccgcgtcgtg ggcgtgctgg agacgagcaa gctgccgacc gaggaggtct
     3361 gccgcaagat cgccgagcaa tgcggcgtcg cgccggagaa gttgacgctg ctggtagccc
     3421 ggacggcaag catcgccggc accgtgcaag tggttgcccg ctcagtcgag acggggctcc
     3481 acaaactgca cgagctgggc tacgacctga aaaacatcat cagcggtttt ggctcagcgc
     3541 cgctgccgcc ggtggctgcc gacgacctcg tcggcatcgg gcgcacgaac gatgcgatcc
     3601 tctatggcgg cgacgtcacg ctgtgggtcc gcgacgacga cgcacgtctc gcgcaaatcg
     3661 gcccgcagat tcccagcagc gcctcgcgcg actacggtga gccgtttgcc gcgatcttcc
     3721 aaaaagccgg gcgcgacttc tacaagatcg acccgctgct gttcagcccc gccgctgtca
     3781 ccctgaacaa cctggcgacc ggcaagtcgc accgcttcgg ccaactgcgg cccgatgtac
     3841 tctcccgctc ctactctgaa ccctgaaccc cgaaccctga accctgcccg tgcgttttgc
     3901 cgtcctcgct gctcccgaaa gctggtacgc ccgcgatttg acgcgggcgg cggcgggcga
     3961 ccacgaagtg gtcacgctgc ccttcagcgc ggtcagcgcc cagatcgacg agcagggcag
     4021 gacgcaagtc ctctgcggcg ggcgcgacct ggcggacttc gacgcggtgc tggtccgcac
     4081 gatgccgccg ggctcgctgg agcaagtcgt cttccggatg gattgcctgg ggcggtacga
     4141 agccgccagc ggcgtcgtca tcaacccggc gagggccgtc gaagcggccg tcgataagta
     4201 cctcacttcc gcaaaactgg ccgcctccgg actgttgacg ccgcggacga tctgttgcca
     4261 gacggccgac gacgcgctgg ccgcgttcga gcaactgggc ggcgacgtcg tgctcaagcc
     4321 gctgtttggc agcgagggcc gcgggatcgc gcggctcaat gacgaagcgc tggccgagcg
     4381 ggcgttcaaa atgctcgcgc agctcggcgc tgtgctctac ctccaggaat tcgtgccgca
     4441 cgaaggctgc gacgtccgcc tgctcgttat cggcgagaga atcctggcca tgcggcggtg
     4501 caatccgctc gattggcgca caaacatcag ccgcggcgcg gcggccgagc cactggtgcc
     4561 cgacgacgcg ctggcggaga ttgcccggcg ggccgccgct gcggtcggcg cgccggtcgc
     4621 cggcgtggac gtgctgcccg gccgcgacgg ccggctctac acgctcgaag tcaacgccgt
     4681 gcccggctgg aagggcctcg ccaaggccca cggcgtcgat gtggcccggc tggtcttgga
     4741 tttcgccgcg agcgaagtcc agagtcggac gcgtcgagag taagagaata acctctgtat
     4801 gcagcagtct gcccccgctg aaacccgact cccgactccc gacccccgac acccggccat
     4861 cgtcaacgtc ggcctgcggc tgagcgaaac ggcccgcctc aatcccaacg gcgtggccgt
     4921 cgtagtgccg cgcgggcgcg atggcggcaa gcggatctac gcgtcgctga cgtttcgcga
     4981 actggacgac gacagcaacc tgctggccga cggcctggcg gcgatgggtg cgcggcccgg
     5041 cacgcggctg gtgctgatgg tcccgccaag catcgacttc atctcgctgg tgttcgcgat
     5101 gttcaaggcg ggcgtcgtga gcgtgctgat cgacccgggc atgggccggc ggaacctgat
     5161 ccgctgtctc gtggaaagcg aacccgaggg cttcatcggc gtaccgctgg cgcaggcggt
     5221 gcgatgcgtg ctgcgggggc gatttccaaa cgccaagctc aacgtgacgg ttggccgccg
     5281 ctggttctgg ggcggccgca cgatcgcgca gctccgcgcg cgaaagctct cggccgactg
     5341 gcggcccgcg gtgaccaagg cggacgatcc ggcagcgatc atcttcacca cgggcagcac
     5401 gggcccgccg aagggcacgc tctaccgcca tggcaatttc gaccgccagg ccgatgagat
     5461 tcgcgacttc taccgcattc agccgggcga aatcgacctg cccggctttc cactgtttgc
     5521 gctcttcaac tgcgcgatgg gcgtgacgac cgtcattccg gacatggacc cgacgcggcc
     5581 ggcgcgggtc gatccgcgaa acatcgtcga agcggtcacg gattggaagg tgacgcaggc
     5641 gttcggctcg ccggcgctgt ggaacgtcgt cggccggtat tgcgaagagc gacagatcgt
     5701 gatgccgaca ctgcggcgag tgatgtcggc cggcgcgccg gttccgccgc acgtgctgca
     5761 gcgaatgaaa gcggcgattg ccccagaggg agacgtgcac acgccctacg gcgcgaccga
     5821 ggcgctgccc gtcgcttcga ttgccgcctc ggaggtgctg gccgagacgg ccgccaagag
     5881 tgctgccggc gcaggcacct gcgtcgggcg gcacttcccg gggatcgact ggaaggtcat
     5941 ccgcatcacc gacggcccgc tcgccacgct ggagcgggcg gaagagcttc ccagcggcga
     6001 gatcggcgag ctgatcgtcc gcggcccggt cgtgaccacg gagtacgtga cgcggaccga
     6061 gtgcaacgcg ctgcacaaga ttcgggacgg ccagtcgttc tggcatcgga tgggcgacgt
     6121 cggctatttg gaacacgagc ggttctggtt ctgcggccgc aagtcgcacc gcgtcatcac
     6181 gccgcgcggg acgctgttca cgattccctg cgaggcgatc ttcaaccagc acccgcgcgt
     6241 ctatcgctca gccctggtcg gcgtcggagg gccggggcaa caacggccgg tgatcatcgt
     6301 cgaaccctgg cccgagaaac gaccgcgatc gacgtccgat gcggcgcagc tgattggcga
     6361 actgcgcgaa ctcggccaac agcacgccca cacggccgag atcggcgact tcatgattcg
     6421 caacgcatta ccggtggaca ttcgccacaa cgccaagatc ttccgcgagc agctcgccat
     6481 ctgggcagcg tggcagctcg gcaccacagc gcaggcgtga ctcattcctc gtcgccggcc
     6541 cgcacatggc agtagctctc gtaccgccgg gcgtcgaggc ggttgtcggc caccgcgtcc
     6601 ttcacggcgc agtcggcctc gtgcgtgtgc gtgcaatcgg gaaaccggca gcggctgacg
     6661 taaggccgaa tatcgcgata gagtccggcg acttcctcgg gcacgatgtc ccacagctga
     6721 aactgccgaa tgcccggcgt atcgaccacg aacccaccaa tcgccagcgg aatcagccgg
     6781 gcgacggtcg tggtgtgttt gcccttctgg ttttcgcggc tgacctcgcc gaccttgagg
     6841 gccagctccg gctcgatggc gttgagcagc gacgacttgc cgacgccgct ctgcccggcg
     6901 acgacgcttt ccttgccggc caccagccgc cgcagctggt cgatgccctg gccggtcgta
     6961 gcgctgacca gcagcacccg gtaacccatc tggccataca cgccggcgaa gggctgcaga
     7021 tcggccggat cgaccaggtc gatcttgttg atgcagagga tcggctcgat ccgcatctgc
     7081 tcggcctcga ccagaaagcg gtcgatcagg ttcggcttca gcgtcggctc ggcggcgctc
     7141 gttacgatca cgagttgatc gacgttggcg acgaggatct gctgccggcc tttgctcgtg
     7201 cggctgagaa tccgcgcacg cggctcgatc cgctcgatca gcgagtcgcc ggttgcttcg
     7261 gggcggatcc agacgatgtc gcccgccacg accacgtgcc gcaggtcggt gctcaggtct
     7321 ttcagcacgc cgcgcacggc gcagcggaac tggcggccgt catcggcctg cacgacgctc
     7381 gacaggccgt gaacgctcag cacgtggccg cggatcgcgg ttccatcgac gtcgcgaagg
     7441 acggcgaacc ccgcggtctc ggcatcggcc tcgacgccga cgatagtccg cttgcgggtg
     7501 aggtcgccct tgccggtcag gcgttcggag cgaacgagcc ggtcctcggc gttttcgtcg
     7561 ccgagcacct gccgcgtcaa atcgccttgg cgcttccggc cctggtgttg cttgcggaat
     7621 tcgtggcgga tttttctctt ccggcccatg cctgcattgt aatgcggacc gtctccgcga
     7681 agctgacgcc accgcagttg gccggtggtg gtcaagcagg cgtcaagctg tccgcactgc
     7741 tgacagcctg gcgaagggca gttgaatgct tggtttccag aatccctgag cctacccaga
     7801 gtccgtgggc ccagacgcgg cacagccttt gcgaatgggt ggcgcatcgt tttcgggggc
     7861 taaaggtccg ctagcagttg cggccattgc gttcggctcc cgaaaatcac ggacccactt
     7921 gcaggaggac cttccatggc tcggaattgg aaacagtggt tggtttttgc agtcatcgga
     7981 ttgagctcgc tggtaatcgt ggctgacgcc gacgcttggc cccggatttt cggccggcgc
     8041 cgtgcccgct cgaacaacac gacctacacg acctataggt acaccggtgg gatgtctgga
     8101 acggcaagtg ccggtgctgg cggaataaat gctgcgacgg acccgaacct ccgcgttcgt
     8161 ggccagacac caggcaccga tgcgaggccc gccgtcccgg ccactacacc ggctcccccg
     8221 acgatcccat agtccgtcgg aaatctgccg tcggtttcgc gcaagcccgc ccgcaccggc
     8281 tgctggcggg cgttcgttgg cagccgtaac ttacgcgccc gcaagcagat agcgccgact
     8341 tgcctcccac aaacgattgc gtgctgggtg tccggcggct atattcaagg gcgtggctga
     8401 atcggttgat gtggtcttct ggagccgcgt cagctttgcc acgcgcccgc cttccagttc
     8461 ccgccctgac gactctgaag atgacgcggc cacggcggca gtggatggcg gctggcgacg
     8521 gtgttcctgc acgggggtgg cgcgatgcac agtcgatggg tggctttggc actttgtatc
     8581 gctcccgcct cagtaattgc tgcagaaccc gcggcagacg ctgacatcag cttcttcaag
     8641 cacgtgctgc ccatcctgcg gcagaagaac tgcacgggct gccatcagcc ggcgaagaag
     8701 ggcggcgagt acgtgatgac cgatttcgcc gcgctgctga agggcggcga gagcggctcg
     8761 gcggcggtcg tgccgggtga ccccaaaaag agctatctca tcgaccagat tacgccgggc
     8821 aaagacggca aggccgaaat gcccaaggag ttgccgccgc tgacttccag cgaaatcgac
     8881 acgattacga agtggatcgc ccagggggcg aaagacgaca cgcccgccag caaccggccg
     8941 cagtacgacg cggcccatcc gccgatctat atggcggcgc cggtgctgaa gtcggtggag
     9001 ttctcgcccg acggcaccct gttggctgtg agcggctatc acgaagtgct gctgcacaag
     9061 gccgacggct ccgctctggc ggggcgtctg gtcggtcagt ccgagcggat cgagtcggcc
     9121 cgcttctctc ccgacggcaa gttcctggcc gtcagcggcg gctcgccggg gcggtttggc
     9181 gaactgcaaa tctgggacgt cgagaaacgc gagctgaagc tctcgctgat gatcggctac
     9241 gacacgctct acggcgcgag ctgggccccc agtgggaaga tggtcgccat cggctgcgcc
     9301 gacaacacgg tccgtgcgtt cgacgccgaa accggcaagc aggttctgtt taacggcgcg
     9361 cacagcgact gggtgctcga cacgaccttc agcgtaggca gcgatcacat catttcggtc
     9421 agccgcgaca tgtcgatgaa gctgatcgag gtcaagaccg agcggttcat cgacaacctc
     9481 acgagcatca cgcccggcgc tctcaagggc gggctgatct ccatcgaccg ccatcccacg
     9541 aaggacgagc tgctctgcgg cggttcggac ggcgcaccca agatcttcaa gatggtccgg
     9601 accgaggccc gcaagatcgg cgacaacgcc aacctgctcc gcgaattcgc cgagctgcca
     9661 ggccgcgtct ttggcgtggc ctacaaccac gacggcagcc gcatcgccgc tggcagctcc
     9721 agcgacggcg tgggcgaagt acgggtctat aacgcgatgg acggggcggt cgtgtggaaa
     9781 gtcagcgtgc ccgaaggggg catattcacg gtcgatttca acaaggacag cacgatcgtc
     9841 gccgccggcg gcttcgacgg cgacgtgcgg ctgtataacg cgacggacgg aacgctaatc
     9901 aagcggttca caccggtgga aattacgggg gccgccgtgg cggcgaatta ggagccagat
     9961 ggtgaagctg aaccaacctc tctgcgtact ctgctgggct acggccctcg tgctaacggg
    10021 gctagtgctt tctttgcgcg gcgacgaagt gccgagcgag aagctccccg cggggctgaa
    10081 cgtcgtggcg ctcgaagcgc ggcctgcaac ggtggagctg acgcataagt ttgactatcg
    10141 ccaactgctg atcaccggca agctcgatag cggcgagacg gtcgatctca cgcggatggc
    10201 gcaggtgtca tcgccgggca ctgccgtggc ggtgtcgaaa gacggcctgg tgcgggccaa
    10261 ggcggatggc accgaggaga tcacattcac gttcggcggc ctttcgacca aggtcgccgc
    10321 caaggtgagc ggcgtcgccc agccgcacgt cgtcagcttt gtgcgcgacg tgcagccggc
    10381 cttgtcgcgg atgggctgca acgcgggcac ttgccacggg gccagggagg gcaaggccgg
    10441 cttcaaattg tcgctgcgcg gctacgacgc gctctatgac caccgggcct tcaccgacga
    10501 tatcggcggc cggcggttca accgcgcggc gccggatcag agcctgatgt tgctcaaggc
    10561 gaccggctcg atcccgcacg tcggcggcgt acggacaaac gtcggcgagc cgtactacga
    10621 gttggtccgc gactggatca accagggagt gaagctcgac ctgaaggccc cgcgcgtgac
    10681 gaagatcgag gttttccccg tcaacccagt ggtaccgcg
//
LOCUS       DASRJB010000903         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3648694, whole genome shotgun sequence.
ACCESSION   DASRJB010000903 DASRJB010000000
VERSION     DASRJB010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648694"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFB80_23835"
     CDS             complement(<1..999)
                     /locus_tag="VFB80_23835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN36890.1"
                     /translation="MAISCTRREVLQSAAAATVAAALQSQAAAQVPRTPRVDAHLHCF
                     AGPLDAKFPYHERAPYKPDQPATPEHLLDCMKQAEVDYAIVVHPEPYQDDHRYLEHCL
                     AVGRGKLKGTLLLFADRAGSLDKLPQLAKLPVVALRVHAYAPDRLPPFGKPELRRLWQ
                     AAGDHGLAVQLHFEPRYAPGFEPLIREFSKTKVIIDHLGRPFQGTPAEHAVVLKWAKL
                     DNTIMKLSAIPEKTTYPHRDIAPVIRQLTDAWGAERMIYGGGFSATATGESYRQAFEK
                     ARSQLTHLSAADQAKIFGHCGPSVFPGDVVSRRPATAMSICTAPQQSRKIRLAIGLL"
     gene            1160..1594
                     /locus_tag="VFB80_23840"
     CDS             1160..1594
                     /locus_tag="VFB80_23840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HZN36891.1"
                     /translation="MWRLLCVGVLVAMTLPAVAADEVDDEMALIGTWTPMKAELAGAA
                     MPDAVLKSIVLKLTEGKYEVTVAGQLDRGTWKLDKAAKPKQLTITGTDGPNKDKTFPC
                     IYELKGDTLRVCYDLSGKMAPAEFATAAGTQLYLVTYQRKKE"
     gene            complement(1721..2035)
                     /locus_tag="VFB80_23845"
     CDS             complement(1721..2035)
                     /locus_tag="VFB80_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36892.1"
                     /translation="MPALSDYTNVFATALQVLRDKGYRVWFDARAGNYCAEKDGWDFM
                     SRSPCGLLGVVAIYEHVKPTQFREYWWRSYLAPDHRQLTRDSPDYIPVYARDKKSTET
                     ND"
     gene            complement(2068..3186)
                     /locus_tag="VFB80_23850"
     CDS             complement(2068..3186)
                     /locus_tag="VFB80_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN36893.1"
                     /translation="MPAAPAAAPPPPTPAAPTPAAQHPLGPLPAPSTFKPQPLQQPQP
                     AGLRPQVAPGAETPTKRLFDRITAEIAKIYVGQDELVLGALVALFSSGHVLIESVPGL
                     GKTLFVRTLGRVMGLKFGRIQFTADLMPSDVTGAPILDMKTQEFRFRQGPVFTQLLLA
                     DEINRAPAKTHAALLEIMQEYRVTVDGKSHPIEKPFLVLATQNPIESEGTYNLPEAQL
                     DRFMFKLVASYPQEGEEAEILKMHSAQRDVTERLDTELATITAAPEILETIQSNAAVR
                     IDERLVDYINKIVRRTREWPQFHMGASPRAGISLMQAARTLAAFSGRDFAVPDDVFQI
                     ALPALRHRVALTAEAEVEGQKVDDLLTELIRSVEVPRL"
     gene            complement(3474..>4476)
                     /locus_tag="VFB80_23855"
     CDS             complement(3474..>4476)
                     /locus_tag="VFB80_23855"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZN36894.1"
                     /translation="HRVTTVSRLSPKLEEFDVVVWAPNDFEPPTKEQREFLEDWLVNG
                     TNRTVIYIGRDYDGAAAYWDRIAPQAPPEQADEMLRRRAEARAAHEAARSKMPVKQYS
                     RWFTVERDKPPKKPAKLTGEWAGAVDPAKTEIHLEGRLSIPTKRDAGPSDPSVPAEIK
                     TLLAADGEGIIYRESDAEEWGDGQVIVVANGSLVLNYPLVNSENRKLAGRLVDECGEK
                     GRVVFLESGPGGPPVLDKEPSAGAAGVLDLLKVWPLNVILLHGTLLGVIFCLARSPIF
                     GRPRELRPESPTDFGKHVAALGQLLARSKDRNYAQARLAHYRQIAERKSGRSHLKNK"
BASE COUNT          737 a         1419 c         1527 g          793 t
ORIGIN      
        1 gagcaaacca atcgccagcc tgattttacg tgattgctgc ggcgcggtgc aaatgctcat
       61 cgcagtcgcc ggccgccgcg acactacgtc gccagggaaa acactcgggc cgcagtggcc
      121 gaaaatcttg gcctgatcgg cggccgagag gtgggtcagt tgcgagcgtg ccttctcaaa
      181 tgcctggcgg tagctctctc cggtcgcggt ggcgctaaat ccgccaccgt agatcatccg
      241 ctcggcgccc caggcgtccg tgagctggcg aatgaccggg gcgatgtccc ggtgcgggta
      301 ggttgttttt tcgggaatcg ccgagagctt catgatcgtg ttgtcgagct tcgcccactt
      361 caggacgact gcgtgctcgg cgggcgtgcc ctgaaatggc cggccgaggt ggtcgatgat
      421 caccttggtc ttggagaact cgcggatcag cggttcgaag cccggggcgt accgcggctc
      481 gaagtgcagt tgcaccgcca ggccgtggtc gccggccgct tgccacagcc gccgcaattc
      541 cggcttgccg aaggggggca gccggtccgg ggcataggcg tgtacccgca gggcgacgac
      601 gggcagtttg gcgagctggg gcagcttgtc gagcgagccg gcgcggtcgg caaacagcag
      661 cagcgtcccc ttcagcttgc cgcggccgac cgccaggcaa tgctccaggt agcggtggtc
      721 gtcctggtag ggctccggat gcacgacgat tgcgtaatcc acctcggcct gcttcatgca
      781 gtcgagcagg tgctcgggcg tcgccggctg atcgggcttg tacggcgctc gctcgtgata
      841 cgggaacttc gcgtcgagcg gtcccgcgaa acagtgcagg tgggcatcga cgcgcggcgt
      901 acgcggaacc tgagcggcgg cctgtgactg cagggccgcg gccacagtcg ccgcagccgc
      961 ggattggagc acttcgcggc gcgtgcagga tattgccatg gtctggcctc cggctagata
     1021 ctgcgatgcg agtcctactc taaccgacgg aacactccgg ttcaccaggc gcgcgcagcg
     1081 acttgatccg gccggtgtgg acggggatga tcttggggtc gctggctcct cgaaacttgc
     1141 ttcatgaagg gagacgatca tgtggcggct tctgtgcgtg ggtgttctgg tcgcgatgac
     1201 actgcccgcc gtggcggcgg acgaagtgga cgatgaaatg gcgctgatcg ggacctggac
     1261 gcccatgaaa gcggaactgg ccggggcggc gatgcccgac gcggtgctga agtcgatcgt
     1321 tctcaagttg accgaaggca aatacgaagt caccgtggcg ggccagctcg accgcggcac
     1381 ctggaagctc gacaaggccg ccaaaccgaa gcagctgacc atcaccggca ccgacggacc
     1441 caataaggac aagactttcc cctgcatcta cgagctcaag ggcgacacgc tccgcgtctg
     1501 ctacgacctc tccggcaaaa tggcccccgc cgaattcgcc accgccgccg ggacgcagct
     1561 ctacctggtg acgtaccaga ggaagaagga ataggcccgg agggccgaca cagaacgaat
     1621 ccgcaggcac gtgccggtcc tccggacctc accgacgtgt tgccgtggct tcgaacccca
     1681 acctcacggt tggggctatt tcctgacggg cctccggccc tcagtcattc gtctcggtcg
     1741 atttcttgtc gcgagcgtag acgggaatgt aatccggaga gtcgcgtgtc aattgtctat
     1801 ggtcgggcgc taagtagctg cgccaccagt actcgcgaaa ctgcgtcggc ttcacatgct
     1861 cgtagatcgc gacgactcct agcaaaccac acggcgaacg agacatgaaa tcccagccgt
     1921 ctttctcagc acaatagttg ccagcgcggg catcgaacca aacacggtat cccttgtcgc
     1981 gcagtacttg cagcgcggtg gcaaagacgt tggtgtaatc ggacaaagct ggcataggac
     2041 ggctctccgc atacgcgaac tgacctccta cagccgcggg acttctaccg agcggatcag
     2101 ctcggtgagc aggtcgtcca ccttctggcc ttcgacctcg gcttcggcgg tgagggcgac
     2161 gcggtggcgg agggcgggca gggcgatctg gaacacgtcg tcgggcacgg cgaagtcgcg
     2221 gccggagaac gcggccagcg tgcgggcggc ctgcatcagc gaaatgccgg cccgcggcga
     2281 ggcccccatg tgaaactgcg gccactcccg cgtgcggcgg acgatcttgt tgatgtaatc
     2341 gaccagccgc tcgtcgatcc gcaccgcggc gttcgactgg atcgtctcca agatttccgg
     2401 ggcggcggtg atcgtcgcca gctcggtgtc cagccgctcg gtcacgtcgc gctgggcgct
     2461 gtgcatcttg aggatttcgg cctcctcacc ctcctgcgga tagctggcga cgagcttgaa
     2521 catgaagcgg tcgagctggg cctcgggcag gttgtaagtc ccttccgatt cgatcgggtt
     2581 ctgcgttgcc agcaccagga acggcttctc gatcggatgg ctcttgccgt ccaccgtgac
     2641 gcggtactcc tgcatgatct ccagaagggc cgcgtgcgtc ttggcgggtg cccggttgat
     2701 ctcgtcggcc aagaggagct gcgtgaagac cggaccctgg cggaagcgga actcctgcgt
     2761 cttcatgtcc aggatcggcg cgccggtcac gtcggacggc atcaggtcgg ccgtgaactg
     2821 gatgcggccg aacttcagcc ccatcacacg ccccagcgtc cgcacgaaca gcgtcttgcc
     2881 caggcccggg acgctctcga tcagcacgtg cccgctggag aagagcgcga ccagcgcccc
     2941 cagcaccagc tcgtcctggc cgacgtaaat cttggcaatc tccgccgtga tccggtcgaa
     3001 cagccgtttg gtcggcgtct cggcgccggg cgctacctgc ggacgcaagc ccgccggctg
     3061 cggctgctgc agcggctgcg gcttgaacgt tgaaggcgcg ggcaatgggc cgagtggatg
     3121 ctgagcggcc ggcgtcggcg cggccggcgt gggaggcggc ggagcagccg caggggctgc
     3181 gggcatggtc caggtcggct ggatcggcgg cgctggctga gccgggtgcg aattgccgcc
     3241 cgccgcgggc tgcgagggcg aactgggcgg ctgcgaagtg gccggcacct gcacgatcgc
     3301 gctacactgc gggcaactgg ccctgcggcc ggccgcgctg gcgggcacct tcagcgcccg
     3361 cccgcagccg gagcagtgga actcgatggt gtcggcggtg gtcgttggtt gggacattgc
     3421 tgaatacgaa tgaccaatga caagtgacaa atgacctggt cattcgtcat tggtcatttg
     3481 tttttgaggt gcgaccttcc cgacttgcgt tcggcaattt gccgatagtg ggccaggcga
     3541 gcctgagcat agtttcgatc cttggagcgg gccagtagct gtcccagggc ggcgacgtgc
     3601 ttgccgaaat cggtgggcga ttcgggccgc aattcgcgcg ggcggccgaa gatcggcgag
     3661 cgggccaggc agaaaatgac acccaagagc gtgccgtgca ggaggatcac gttcagcggc
     3721 cagactttga gcaggtcgag cacgccggcc gccccggcgg agggttcctt gtcgaggacc
     3781 ggcggcccgc ccggcccgct ttctaggaag acgacgcgac ctttctcgcc gcactcatcg
     3841 accagccggc ccgccagctt gcggttctcg ctgttgacca gcggatagtt gaggaccagt
     3901 gagccgttgg cgacgacgat cacctggccg tcgccccact cctcggcatc cgactcgcga
     3961 tagatgatcc cttcgccgtc ggcggccagc agcgtcttga tctcggcggg caccgacgga
     4021 tcgctgggcc cggcgtcgcg cttggtcgga atcgacagcc ggccctccag gtgaatctcg
     4081 gttttggcgg gatccaccgc gcctgcccac tcgccggtga gcttcgccgg cttcttcggc
     4141 ggtttgtcgc gctcgacggt gaaccagcgg gagtattgct tcaccggcat cttggagcgg
     4201 gccgcttcgt gggcggcgcg ggcctcggcc cgccgccgga gcatttcgtc ggcctgctcg
     4261 ggcggcgctt gcggcgcgat gcggtcccaa taagcggccg cgccgtcgta gtcgcggccg
     4321 atgtagatca ccgtccgatt cgtaccgttg accagccagt cctccaggaa ctcgcgctgc
     4381 tccttggttg gcggctcgaa atcgttgggc gcccacacga ccacgtcgaa ctcttcgagc
     4441 ttcggcgaaa gccggctgac cgtcgtcacg cggtgc
//
LOCUS       DASRJB010000904         5926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1656023, whole genome shotgun sequence.
ACCESSION   DASRJB010000904 DASRJB010000000
VERSION     DASRJB010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656023"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VFB80_23860"
     CDS             complement(<1..1652)
                     /locus_tag="VFB80_23860"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HZN36895.1"
                     /translation="MPIRIYALAKDLKIDSKELVEFCNKAGIPGKGSALASLEDDEVV
                     KLRAWMDASATKRQAAGPAPLVDTKAVLSTPVAGTATLPPPPPPTRPSAVPVRPRPAA
                     TPVIPPTPPPAPAKTAESEAPAISAKGTAVVELPPRPAQAPQPSVTAPTARVAAPLPP
                     TAEPAPVRGDSLFRPRGSGIKVLDAKTKRPATDGRKKDEGGAGKPPERRGPGGSPRFA
                     AMPEVKQPTPSAKTTEERVQKPILKLPTDVLKGSKSSRAAPLQALAEKVDRERKQKDT
                     ADKLGGLTGADGKGPLTAKGKGRKGRGDEEEIRGKGLADMASIRTDRTKGRKAKSTET
                     GDDEVAPRRKRTLVRSKSHGSTAAPRKGKVALELPATVRSFSEATGVSSGQVLKTLMG
                     LGMAVTINAPIPNDYLELLIGELGVEVEFKAAQSLEEKLLARFEQEEEEDAASLTPRP
                     PIVTVLGHVDHGKTSLLDKIIGTNVVAGEAGGITQHIRAYLIETKDGRHVSFVDTPGH
                     EAFTEMRARGANVTDIAVLVVAADDGIMPQTEEAISHAKAAEV"
     gene            2133..2600
                     /locus_tag="VFB80_23865"
     CDS             2133..2600
                     /locus_tag="VFB80_23865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN36896.1"
                     /translation="MHIRPFKPADLDALKGITVDGFTGVSIDHGIEQAFGPINGHDWM
                     WRKARHIDLDVARDSAGCFVADDQGTIAGYITTWQDPEAGIGHIPNLAVAPAYRNHGL
                     GRTLIEHALGHFRAAGLTHAKIETLAQNAIGNHLYPSLGFVEVARQVHFIAKL"
     gene            complement(2669..>5926)
                     /locus_tag="VFB80_23870"
     CDS             complement(2669..>5926)
                     /locus_tag="VFB80_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN36897.1"
                     /translation="QDRPGKDSIRVLEDTDGDGRFEKVTTFADGLNIPIGIYPYKGGC
                     IAWSIPNIWHFEDADGDGVCDKRTVLYGPFDHTRDVHGNCNAFRRGFDGWVYACHGFN
                     NDSRVKGRDGNEVHMQSGNTFRFQLDGSRIEHFTHGQVNPFGMCFDEQFNIFTADCHS
                     KPLTQLLRGGYYPSFGKPHDGLGFVPSMMDHSHGSTAICGVAIYSGENFPPEYRGLFY
                     SGNVMTSRINCNKPEYHGSTIRAIEQPDFLSTSDPWFRPVDIHIGPDGAMYILDFYNR
                     IIGHYEVPLPHPGRDRTSGRIWRVAYKGAVGNALRGIPGRGTDLTKLTIPQLIEQLDS
                     PQLEHRLRVLNYLADQRAKECALPATRAFESPRSPPQQAAAMWLLRRCASFSQQRLRA
                     AALHSNRDVRLHALKMLADSAALDEGDLSLASARTKDEDAFVRRAAAEVLGAHPAPAQ
                     FGGLVSRLPAIAEDDALFRQAVRLAIREVLQVMDSDAGWAIAERLGGTKEQMQEVAGL
                     AASAQSAGAAALLVSYLGRLETLPAIDYERLASNIPAASVERLVAICRERSTGDVQSQ
                     AVLIRSLAAGLSQRNPVAAPVLQAWATQICSEILGASSPESVPWNAVPIDGLPPSENP
                     WVVAARPSNDGDKDSLFYYSLPKGEQRTGIYRSGAFELPAKLSFWCAGHSGFPGRPLN
                     DGNYVRLRDAKTHELLAESRPPRNDTARRFEWDLSKFVRPEGEAPAEPRRGYIELIDG
                     DTANAYAWLAVGRFSLASLNPGQAAGRQQLAAEIVGKLKLTILKPQLTSLVSAPNTDR
                     TARAAIAQALVAMKPDARAAALAVALGDPQTPAVLQVKIGAAIERNVEGTYLEALRDV
                     MKQAPTRLQAQLADALAGDTSGAAALIALVESGLAAPRLLLLPNVKNKLAALGSQNLD
                     QCVAKITAALPPASETIDRLLLERRVAFAKAMPNHERGLALFTKHCAACHQIGGKGAV
                     VGPQLDGIGSRGLDRLLEDTLDPNRNVDVAFRTTTIRTKDGQVLTGLVRREEGPQLVL
                     ADAQGKEFTVAKSEIDGQQKTNLSLMPANVGEVVTAEEFADLIGYLLAQRQAANK"
BASE COUNT          907 a         1976 c         1947 g         1096 t
ORIGIN      
        1 acttcagcgg ccttggcgtg gctgatcgcc tcttccgttt ggggcatgat gccgtcgtcg
       61 gcggcaacga ccagcacggc gatgtccgtg acgttggcac cgcgggcccg catctcggtg
      121 aaggcttcgt ggcctggcgt atcgacgaac gatacatgcc ggccgtcctt ggtctcgatc
      181 aggtaggctc ggatgtgctg cgtgatgccg cctgcctcgc cggccacgac gttggtgccg
      241 atgatcttgt ccaacagcga tgtcttgccg tgatcgacgt ggcccagcac cgtgacgatc
      301 ggcggccgcg gcgtcaggct tgccgcatcc tcttcttcct cctgctcgaa tcgcgccagc
      361 agcttttcct ccagcgactg cgcggccttg aactccacct ccacgcccag ctcgccgatc
      421 agcaactcga gatagtcgtt ggggatcggc gcattgatag tgaccgccat gcccaggccc
      481 atcagcgtct tgagcacttg cccggagctg actcccgtgg cttcgctgaa ggagcggacg
      541 gtggcgggca attccagcgc caccttcccc ttgcggggcg cggcggtgct gccgtgcgac
      601 ttgctgcgga cgagcgttcg cttgcggcgc ggagcgactt cgtcgtcgcc cgtttcggtg
      661 gacttggcct tccgcccctt ggtgcggtcg gtccggatcg aggccatgtc ggccagaccc
      721 ttgccgcgga tttcctcttc gtcgccccgg cctttgcggc ctttcccctt ggcggtcagc
      781 gggcccttgc cgtcggcgcc cgtcaggccc ccgagcttgt ctgccgtgtc tttttgcttc
      841 cgctcgcgat cgaccttttc cgccaaagcc tgcagcggcg ctgcccggct cgacttgctt
      901 cccttgagga cgtccgtcgg cagcttcagg atcggcttct gaaccctttc ctcggtggtc
      961 ttggcggagg gcgtcggctg cttgacctcg ggcatcgcgg cgaaccgcgg cgaaccgccc
     1021 ggaccgcggc gctccggagg cttgcccgcg cctccttcgt ctttcttgcg gccgtcggtc
     1081 gccgggcgtt ttgtcttggc gtcgagcacc ttgatgccgc tgccgcgggg ccgaaacagc
     1141 gaatcgcctc gaaccggcgc gggttcggca gtcgggggca gcggcgccgc gacgcgagcc
     1201 gtcggcgcgg tgacggacgg ctgcggcgct tgcgctggtc gcggcggcag ctccaccaca
     1261 gccgtgccct tggcggaaat cgcaggcgct tcggactctg ccgtcttggc cggagcagga
     1321 ggcggagtcg gcgggatgac cggcgtggcg gcgggccgcg ggcggaccgg aacggccgaa
     1381 ggccgcgtgg gtggcggcgg cggaggcagc gtggccgtac cggcgacagg cgtcgagaga
     1441 actgccttcg tatcgaccag cggggcgggg ccggcggcct ggcgcttcgt cgccgacgca
     1501 tccatccagg ccctgagctt gacgacctcg tcgtcttcca gactggccag cgcagagcct
     1561 ttaccgggga tgcctgcctt gttgcagaac tccaccagct ccttgctatc gatcttcaaa
     1621 tctttggcga gcgcgtaaat ccgaatgggc actgttgccc tcccttatgc ccgcgggccc
     1681 tggccagctg gccgcacgcg ggactttgta tctgctattt catggtcgcg ttctcctgtg
     1741 cctggtacgc ccccgcaacc caactggggc gacgcttcct tcccactgca attgccaagc
     1801 cgagggtggg tcgaaaaacg acctgccctg cggcagcagg ccgcggaccc gcacagtcca
     1861 cggccttcac cggacgacac ttcggtgctg cgggtgactg gcacccttct accgacaccg
     1921 ccgcccaacg cctgcatccg agcgagtcgg cgacacgcga agcggccagc cgtacccgcc
     1981 cgttggggag tgataagaaa gtgtaccgga cattcccagg caatgccaga cggggaacgc
     2041 ggccaaagat ttcagattta ggatttcaga tttcagattt atgatgcact tgcgtctgcc
     2101 gcacatctga aatctcaaat ctgaaatctg aaatgcacat ccgccctttc aaacccgccg
     2161 atttggacgc tttgaagggc atcaccgtcg atggcttcac gggtgtgtca atcgatcacg
     2221 gcatcgagca ggcgtttggg ccgatcaacg gccacgattg gatgtggcgg aaagcccgcc
     2281 acatcgactt ggacgtcgcc cgcgattccg ccggctgctt cgtcgccgac gaccagggaa
     2341 cgatcgccgg ctacatcacg acctggcagg atccggaggc ggggatcggc cacattccga
     2401 acctcgcggt cgcgccggct taccgcaatc acggactggg ccgcacgctg atcgagcacg
     2461 cccttgggca ctttcgcgcc gccggcctca cgcacgccaa aatcgagacc ctcgcccaga
     2521 acgccatcgg caaccacctc tacccgtcgc tcggcttcgt cgaggttgcc cggcaagtgc
     2581 acttcattgc caagctctga agttgcggat tcgggagggc gaacctccga gccgcggctc
     2641 ggcggagcct cgccctccca cgccgccgct atttgttcgc cgcctgcctt tgcgccagca
     2701 agtaaccgat caggtccgca aactcctcgg ccgtaacgac ttcgccgacg ttggcgggca
     2761 tcagcgagag gttcgtcttc tgctggccgt cgatctcgct cttggcgacc gtgaactcct
     2821 tgccctgcgc gtcggccaga acgagctgcg ggccttcctc ccggcggacc agtccggtaa
     2881 gcacctggcc gtccttcgta cgaatggtcg tcgtgcggaa ggcgacatcg acgttgcggt
     2941 tcggatcgag ggtgtcttcg agaagcctgt cgaggccgcg gctgccgatg ccgtcgagtt
     3001 gcgggcccac gacggcgccc ttgccgccga tttgatggca ggccgcgcag tgcttcgtga
     3061 acagggccag tccgcgctcg tggttgggca tcgccttggc aaacgcgacg cggcgctcca
     3121 ggagcaagcg atcgatcgtc tcgctggccg gcggcagcgc ggcggtgatc ttggcgacgc
     3181 actggtcgag gttctggctg cccagggcgg caagcttgtt tttgacgttc ggtagcagca
     3241 gcagccgcgg ggccgccagg cccgattcga cgagagcgat gagcgccgcc gcgccgctcg
     3301 tgtcgccggc gagcgcatcg gcgagctggg cttgcagccg cgtcggcgct tgcttcatga
     3361 cgtcgcgcag ggcttcgaga taggtgccct ccacgttgcg ctcgatcgcc gcgccgatct
     3421 tcacttgaag tacggcggga gtttgcggat cgccgagcgc cacagccagc gccgccgcgc
     3481 gggcgtcggg tttcatcgcg accagggcct gtgcgatcgc cgcgcgggcc gtgcgatccg
     3541 tattcggcgc tgagaccagc gatgtgagtt ggggcttgag aatcgtcagc ttgagtttgc
     3601 cgacgatctc ggcggctagc tgctggcggc cggccgcctg gccggggtta agcgaggcga
     3661 gcgagaagcg gccgacggcc agccaggcat aggcattggc cgtgtcgccg tcgatcagct
     3721 cgatgtaacc acgacgcggc tcggcaggag cctcgccctc tgggcgcacg aatttgctca
     3781 gatcccactc gaaccgccgc gccgtgtcgt tccgcggcgg ccgcgactcg gccaacaact
     3841 catgcgtctt cgcatcccgc agccgcacgt agttcccgtc gttcagcggc cggcccggaa
     3901 agccgctgtg gcctgcgcac cagaagctca gcttcgccgg cagctcgaac gcgccgctgc
     3961 ggtagatgcc cgtccgctgc tctcccttcg gcaggctgta atagaacagc gaatctttgt
     4021 cgccatcatt gctcggccgc gccgcgacga cccagggatt ctccgaaggc ggcaggccat
     4081 cgatgggtac ggcgttccag ggtacggatt cgggcgagct ggcgccgaga atctcgctgc
     4141 aaatctgagt cgcccacgcc tgcaggacgg gggcggcgac gggatttcgc tgcgaaaggc
     4201 cggccgccag ggaccggatc agaacggcct gcgactgcac gtcgcccgtg ctccgctcgc
     4261 gacaaatggc caccagccgc tccacgctgg ccgcgggaat gttactggcc agccgctcat
     4321 agtcgatggc gggcagcgtt tccagccggc ccagatagct caccaagagg gccgccgcgc
     4381 cggctgactg ggccgacgct gccagccccg ccacttcctg catttgctcc ttggtacctc
     4441 ccaatcgctc ggcgatggcc cagccggcgt cggaatccat cacctgcagt acttcccgga
     4501 tcgccaaccg aacggcctgt cgaaacaacg catcgtcctc ggcgatggcc ggcagccgcg
     4561 agaccaggcc gccaaattgg gcgggcgcgg ggtgcgcacc caggacctcg gccgcggccc
     4621 gcctgacgaa cgcatcctcg tccttggtgc gggcactcgc caggctcaag tcgccctcat
     4681 ccaaagcagc cgaatccgca agcattttca gggcgtgcag gcggacatcg cgattggaat
     4741 ggagggctgc cgccctgagc cgctgttggc tgaagctcgc gcagcgcctg agcagccaca
     4801 tcgcggcggc ctgctgcggg ggactgcgcg gagattcgaa ggcccgcgtc gccggtaggg
     4861 cgcactcttt ggctctctgg tcggcgaggt aattcagcac ccgcaggcgg tgttccagct
     4921 gtggcgaatc aagctgctca atcagctgcg ggatggtcag tttggtcagg tcggtcccgc
     4981 ggccgggaat gccacggagg gcattcccta cagcgccctt gtaggcaact cgccagattc
     5041 gcccgctggt gcggtcgcgg ccggggtgcg gcagggggac ttcgtagtgg ccgatgatcc
     5101 ggttgtagaa gtcgaggatg tacatcgccc cgtcggggcc gatgtggatg tcgaccgggc
     5161 gaaaccaggg atcgctcgta ctgagaaaat ccggctgctc gatcgcccgg atcgtgctgc
     5221 cgtgatactc aggcttgttg cagttgatgc ggctggtcat cacgttgccg ctgtaaaaga
     5281 gtccgcgata ctccggcggg aagttctcgc cgctgtagat cgcgacgccg cagatggccg
     5341 tgctgccatg cgaatggtcc atcatcgagg gcacaaagcc tagcccgtcg tgcggcttgc
     5401 cgaagctggg gtaatagccg ccgcgcaaga gctgcgtcag cggcttgcta tggcagtcgg
     5461 ccgtgaagat gttgaactgc tcgtcaaagc acatcccgaa cgggttcacc tgcccgtgcg
     5521 tgaagtgctc gatgcggctg ccgtcgagct ggaagcgaaa tgtgttgccc gactgcatgt
     5581 ggacttcatt gccgtcacgg cccttcacgc ggctgtcgtt gttgaagccg tggcaggcat
     5641 agacccagcc gtcgaacccg cggcggaagg cgttgcagtt gccgtggacg tcgcgggtgt
     5701 ggtcgaacgg gccgtagagg accgtccgct tgtcgcacac gccgtcgccg tccgcatcct
     5761 cgaaatgcca gatgttcgga atgctccacg caatgcagcc gcctttgtag ggatagatgc
     5821 cgatcggaat attcaacccg tcggcgaagg ttgtgacctt ctcgaaccgc ccgtcgccgt
     5881 cggtgtcttc cagcacccgg atcgaatcct tccccggccg gtcctg
//
LOCUS       DASRJB010000905         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2526785, whole genome shotgun sequence.
ACCESSION   DASRJB010000905 DASRJB010000000
VERSION     DASRJB010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2526785"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFB80_23875"
     CDS             complement(<1..976)
                     /locus_tag="VFB80_23875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36898.1"
                     /translation="MSASVATLPIAANKPQRAASTEPRLWPAVVVVLAYWIVWALVAA
                     FSSNPFAKFMTLFFGPMLLAAAILGWWLFFSRLPWINRFWGVGCQLLLAVVAYLLAHA
                     SVNWMGLLMYATPVALTGAVVWLVVTRGARTLVGWLGLPAIAALAFGYADLIRIDGIT
                     GNLDAERSWRWTTTAEERFLAGQKPAAAPAESAPEATAPPALSPLVASAGDWPEFRGP
                     NRDGHVRGVTIETDWQKNPPREVWRRLVGPGWSSFAVIGNVAYTQEQRGPSEAVVCFD
                     VNTGREHWSHLDTARFEEIVAGAGPRATPTFHDGKLYALGGRGTLNCLD"
     gene            complement(1163..1786)
                     /locus_tag="VFB80_23880"
     CDS             complement(1163..1786)
                     /locus_tag="VFB80_23880"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZN36899.1"
                     /translation="MSTAGNSTATADDSTVENPTAGKSSAANSYEVFKQSLKQAEAAG
                     DAAWAPESSEPTTGSVLGNVELKGLGSAVLLGLGFLIAAGVLLLLNFSHLTRGERTVG
                     VVVGHKDYYNRRSCTVHAPVVNYSAPGGVYKVIGCLSVSRSIYPVGKEVPVLYLRDDP
                     SNAVVADFVQMFLIPTVVGSFGLLCLTVSIGIMIWAVRKGSYGLLAG"
     gene            1942..2646
                     /locus_tag="VFB80_23885"
     CDS             1942..2646
                     /locus_tag="VFB80_23885"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZN36900.1"
                     /translation="MNPPEIVVFDAPHPCSYLPGRTARLPHRHPVQRLAPEEFDQRLA
                     EGDRRTGVFLYRTQCPQCRACEPIRLDVARFRPNATQRRIARKGNELLTVRIAEPTVD
                     EQRTALFNRHRDERGLAGGDAPIDEASYTDFLTLSCVTTLELSYWHAGQLVAVAIADA
                     GRHSLSAVYCYYDPHFRLLSLGTYSILRQVALCRASGRQHLYLGFYVSQSPHMSYKAN
                     FRPHQRLIDGRWIDFG"
     gene            2802..>3054
                     /locus_tag="VFB80_23890"
     CDS             2802..>3054
                     /locus_tag="VFB80_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36901.1"
                     /translation="MARNVLRGNSSRGLRGFTLVELLVVIAIIGVLVALLLPAVQSAR
                     EAARRAQCANNLKQIGLGMHNYVDTWGRLPSALMGGLTSA"
BASE COUNT          549 a         1039 c          979 g          487 t
ORIGIN      
        1 cgtccaggca gttcagcgtg ccgcggccgc cgagggcgta gagcttaccg tcgtgaaacg
       61 tgggcgtcgc ccgcgggccc gcaccggcca cgatttcctc gaagcgggcc gtgtccaggt
      121 gcgaccagtg ctcgcgaccc gtgttgacgt cgaaacagac gacggcttcg ctcggtccgc
      181 gctgttcctg cgtgtaggcc acgttcccga tgacggcgaa cgacgaccag ccggggccca
      241 cgagccgccg ccacacttcg cgcggcgggt tcttttgcca atcggtctcg atggtcacgc
      301 cgcggacgtg gccgtcccga ttggggccgc gaaactcagg ccagtcgccg gcggatgcga
      361 ccagcggcga cagggccggc ggcgcggttg cttcgggcgc cgactccgcg ggcgcggcag
      421 cgggtttctg gccggcaagg aaccgctcct cggccgtcgt ggtccagcgc cagctgcgct
      481 cggcgtccag gttgccggtg ataccgtcga tgcgaatcag gtcggcatag ccgaacgcca
      541 gcgccgcgat cgctggcaag cccagccagc cgaccaaggt ccttgccccg cgggtgacga
      601 cgagccagac gactgcgccc gtcagtgcca cgggcgtggc gtacatcagc aggcccatcc
      661 agttcaccga cgcgtgcgcc agcaaatacg ccacaacggc cagcagcaac tggcagccga
      721 cgccccagaa gcggttgatc caggggagcc tgctgaaaaa cagccaccag cccaggattg
      781 cagcggccag caacattggg ccaaagaaga gcgtcatgaa cttggcgaac gggttgctgc
      841 tgaaggccgc cacgagcgcc cagacgatcc agtaggccag gacgacgacg acagcgggcc
      901 aaagtcgcgg ctccgtggag gcggcccgct ggggcttgtt cgcagcaata ggaagggtgg
      961 caacgctggc gctcatgcga agtttccaga atcgaggaaa cggaaaagcg acggacattc
     1021 tatcgcagcc gcagatcgcg ggcactcgca ggatgctccg ccagccaaat ggttcgccgc
     1081 cgtccctcgc cctgagcgca acctcaatcg acgaacgcgc ttctgcccca gcgccgcagg
     1141 caggataagc ccgctacagg atttagcccg ctagcagccc gtagctgccc ttgcgaaccg
     1201 cccagatcat gatcccgatg gagaccgtga ggcagagcag gccgaagcta ccgacgaccg
     1261 tcgggatcag gaacatctgc acgaaatcgg cgaccaccgc gttgctcggg tcatcgcgta
     1321 agtagaggac cggcacttcc ttgcccaccg gatagatgct gcgggacacc gagaggcagc
     1381 cgatgacctt atacacgccg cccggcgcgg aatagttgac gaccggcgca tgtaccgtgc
     1441 acgagcggcg attgtaatag tccttgtggc caacgacgac gcccaccgtc cgctcgcccc
     1501 gcgtgaggtg gctgaagttc aggagcagga ggacgccggc agcaatcagg aagcccaaac
     1561 cgaggagcac ggccgagccc aggcctttca gctccacgtt ccccagcacg cttcccgttg
     1621 tcggctcgct ggactcgggc gcccaggccg cgtcgcccgc ggcctcggcc tgcttcagcg
     1681 actgtttgaa gacttcgtac gaattagccg cagaggattt acccgcagtg ggattttcta
     1741 ccgtggaatc atccgcagtg gccgtggaat tacccgcagt ggacatggaa caccccctgc
     1801 ggaaatctag ctcaaaagta atcttcgcgc ctggcgagcg gccgacctac gtgggcggcc
     1861 cgacaatgct cgcaaccgtc accagcggac cctcaggccg ccgcgtgata aaatgcaacc
     1921 agtcacgttc tccgcccagc catgaacccg ccggaaatcg tcgtcttcga cgcaccccat
     1981 ccgtgctcct atctgcccgg acgcacggcc cggctgcccc accggcatcc cgtgcagcgg
     2041 ctggccccgg aggagttcga ccagcggctg gccgaggggg accgccgcac gggagtgttc
     2101 ctctaccgca cgcaatgccc gcagtgccgg gcctgcgagc cgatccggct ggacgtcgcc
     2161 cggttccgcc ccaatgccac gcagcggcgg atcgcccgca aggggaacga gctgctgacc
     2221 gtccgcatcg cggagcctac ggtggacgag cagcgaaccg cgctgttcaa ccggcaccgc
     2281 gacgagcgcg ggctggccgg cggcgacgcc ccgatcgatg aagcgtctta cacggacttt
     2341 cttacgctct cctgcgtgac cacgctggag ctgagttatt ggcatgccgg acaacttgtc
     2401 gcggtagcca tcgccgacgc cggccgccat tcgctctccg ccgtgtattg ctactacgac
     2461 ccgcatttcc ggctgctgag cctgggcacc tattcgatcc tgcggcaagt ggcgctgtgc
     2521 cgcgcgagcg gccggcagca tctctatctc gggttctacg tctcccagtc gccgcatatg
     2581 tcgtacaagg cgaacttccg gccgcaccag cgcttgatcg acgggcggtg gattgatttc
     2641 ggctagcgcg ccatttttca cgagcgcttc atttttggtt cacacggccg ggccagattc
     2701 tggggcgtcc tctccaacca cgacccgaac ctggggtctg actgcggtcg tgaaacggca
     2761 ggtttcgcgc ccgcggtttg accatgtgaa ggagaacgct catggctcgg aacgttctgc
     2821 gcggtaactc cagccgcggc ctccgcggtt ttacgctcgt ggaactgctg gttgtcatcg
     2881 cgatcatcgg cgtgctggtg gcgctgctgt tgccggcggt gcaatccgcg cgggaagcgg
     2941 cccgccgcgc gcagtgcgcc aacaacctca agcagatcgg cctgggcatg cacaactacg
     3001 tcgatacgtg gggccggctg cccagcgcgc tgatgggcgg actgaccagt gctg
//
LOCUS       DASRJB010000906         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3374238, whole genome shotgun sequence.
ACCESSION   DASRJB010000906 DASRJB010000000
VERSION     DASRJB010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3374238"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VFB80_23895"
     CDS             complement(<1..1253)
                     /locus_tag="VFB80_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36902.1"
                     /translation="MRKQALRLAYFARGLDGLFLPPVAGCETFGLLRGNSPEEGWSGG
                     LVPAEQSLVYRLRGHADDPYPATGDVLRDSNIGPLLSRRKIGSLLLSSPFTPQVREWS
                     RRFGVRILAAPYEQQQRLENKLTFDRFLARHTIPRPDSAIVIAGEPPSPTARAIRGPV
                     VAQRADSMGGEGTFYLASAAEWPELAGRLQLPRGERCLMRQYVEGRPLGITVFVAPGV
                     VVLSAVRLQCYYPAATDDARRLFAGIQWLKSHEFSRPLMAGISAVFQRLGTLLHQRRY
                     FGFANFDFLVDGGDQVWIIECNPRMSAATPQLLANPALSGGAPLGRMFVEGCLTARKK
                     WPRHVECTPLPDADYVGATLDITSLTPAEELVRREYASGPRRRPEGVIQIFSFAARGQ
                     RAAPETTLATILADFPLYGGRGDLNA"
     gene            complement(1246..1875)
                     /locus_tag="VFB80_23900"
     CDS             complement(1246..1875)
                     /locus_tag="VFB80_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36903.1"
                     /translation="MALLSLSLPAAGPAFGWQAGRFHAVDSLHIRRGSCGAFTNVAAW
                     LAWRLPGLEPGELFHLCPFLDGCAIHRLVRSPGGVAAWVQELGGEFQTQCSLPLRRGS
                     LLFQANCVRQSGSRLAREEALSAAERKPYCDRRERTEQAVRELVRAGETTPQRIVELL
                     ASRAGGDYAYANSDVKAHCVGVATAAGMELYVQSGAALPGDKYRPQFHA"
     gene            complement(2383..3033)
                     /locus_tag="VFB80_23905"
     CDS             complement(2383..3033)
                     /locus_tag="VFB80_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36904.1"
                     /translation="MPDIVLENRKVLSTWRVPLSRRYVARFGAPVVTHVDPLIFLRKF
                     FAYCMDCEFCHDSCCRFGCDTSLATVEKLETQFGDALEQYTGTSRADWFEPEVYADRD
                     FPGGRFRSTRVVNGTCIFKNKERRGCGIHSYCVEHGLDYHDVKPVLCWLFPLTVESDA
                     LCPQNVVLEKSLVCVDQGITLYQSQRGEVGNLFGPELVAELDELERQTLADSHAQP"
     gene            3166..>3397
                     /locus_tag="VFB80_23910"
     CDS             3166..>3397
                     /locus_tag="VFB80_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36905.1"
                     /translation="MICGEESAICIMRFMRASAATRALPPEVSRDHWSQLWAHARICG
                     KLFLHAVIVVTPYSIVIYGYKSGFCIMRFMRAG"
BASE COUNT          585 a         1149 c         1132 g          531 t
ORIGIN      
        1 gcattgaggt cgccccgccc gccgtacagc ggaaagtccg ccaggatcgt ggccagcgtc
       61 gtctcgggcg ctgcccgctg cccacgggcg gcaaacgaga agatctgaat gacgccctcc
      121 gggcgccggc gcgggccgct ggcgtactcg cggcggacga gctcctccgc tggagtgagc
      181 gacgtgatgt ccagcgtcgc gccgacatag tccgcatcgg gaagcggcgt acactcgacg
      241 tgccgtggcc acttttttct agccgtcagg caaccctcca cgaacattcg tcccagcggc
      301 gccccgccgc tgagggccgg attggccagg agctgcggcg tcgcggccga cattcgcggg
      361 ttgcattcga taatccacac ctggtccccg ccatcgacca gaaagtcgaa gttggcgaag
      421 ccgaaatagc ggcgctggtg gagcagcgtt cccagccgct gaaataccgc gctgatgccg
      481 gccatgagcg gccgcgagaa ttcgtggctc ttcagccact ggattcccgc aaacagccgg
      541 cgggcgtcgt cagtagcggc cgggtagtag cattgcagcc gcacggccga gagtacgacg
      601 acgcctggcg cgacgaatac cgtgatacca agcggccggc cctcgacgta ctgccgcatg
      661 aggcagcgct cgccgcgcgg cagctgcagg cgcccggcaa gttcaggcca ctcggctgcg
      721 gatgccagat agaaagtccc ctcgccgccc atcgaatcag cccgctgcgc gacgaccggc
      781 cccctgattg cccgcgctgt cggcgatggg ggctcgcctg cgatcacgat cgccgaatcg
      841 ggccggggaa ttgtgtgccg cgccaaaaag cggtcgaagg tgagcttgtt ttccagccgc
      901 tgctgctgct cgtagggggc ggcgagaatg cggacgccaa accggcggga ccactcgcgg
      961 acctgcggcg tgaatgggct gctgagcagg agtgagccga tcttgcgccg cgacagaagc
     1021 ggcccgatat tcgagtcgcg cagcacatcg ccggtcgccg gataggggtc gtcggcgtgc
     1081 ccgcgcagcc ggtaaaccag cgactgctcg gcgggcacca ggccgcccga ccagccttcc
     1141 tccggcgaat tgccgcggag cagcccgaac gtctcgcagc cggcgacggg aggcaagaac
     1201 aagccgtcca ggccccgcgc gaaataggcc agccgcaaag cttgcttacg catggaactg
     1261 cggccggtat ttgtcgccgg gcagagccgc tccggattgg acgtagagct ccatccccgc
     1321 ggctgtcgcc acgccgacgc agtgggcctt cacgtcgcta ttggcatagg cataatcgcc
     1381 gccggcccgc gaagcgagca gctccacaat ccgctgcggc gtcgtttcgc cggcgcggac
     1441 cagctcccgc accgcttgct ccgtccgctc ccgccggtcg cagtacggct tgcgctcggc
     1501 ggcgctgagc gcctcctcgc gagccagccg gctgccggac tgccgcacgc agttggcctg
     1561 aaacagcagg ctgccccgcc gcagcggcag tgagcactgc gtctgaaact ccccacccag
     1621 ctcctgcacc cacgcggcca ctccaccggg cgagcggacc agacgatgaa tcgcgcagcc
     1681 gtcgaggaac gggcacaggt ggaacagctc gccgggctcg agcccgggca gccgccacgc
     1741 cagccacgcg gcgacattcg tgaaggctcc gcagctgccg cggcggatgt gcagcgaatc
     1801 gaccgcgtga aagcggccgg cttgccagcc gaacgccggt ccggcagcag gtagggatag
     1861 agaaaggagt gccacgtttg tttgcccacc aggagcgacg ccaggcgcgg ccggtcgaag
     1921 tagccgatgg tgtcttcctc ggtgtgccaa aacagcacgt cgccgccggc caatcgcaac
     1981 aggccggtct ggcagccgct gttgtcgtcc tggagccaga gagccagctc ccgcgcggtg
     2041 acggctgagc cgccggccgt ccggccgcgg agtacggatt ccaattggct ttcctgggtc
     2101 caggcgtcga ggcacgccag gtactcgcgc aaggcccggc ggacgtgccg cggcatgaag
     2161 tgaccgctgc ggatgtacgc cgacacatgc cgccggcagt tgcggacggc gtcgatttgc
     2221 cggtcgaaat cggccggcga agtcgctcgc agccgccgcg ccgcaacgcg gcggatcacc
     2281 gggcccagcg cttcgcccag ccggccgtgt gccgccggca gcgacgctgc ttcgatctcg
     2341 atccgccgca gcggacccag gcggcggcgc tgctctggca ggctagggct gggcatgaga
     2401 atcggcgagc gtttgccgct ccagctcgtc cagctcggcg accagctcgg ggccgaacag
     2461 gttgccgact tcgccccgct ggctctgata gagcgtgatg ccctgatcga cgcagaccag
     2521 cgacttctcg agcacgacgt tctgggggca aagggcgtcg gactcgaccg tcagcggaaa
     2581 cagccagcag agcaccggct tgacgtcgtg atagtccagg ccgtgctcca cgcagtacga
     2641 gtggatgccg cagccgcggc gctccttgtt cttgaagatg caggtgccgt tgaccacgcg
     2701 cgtcgagcgg aagcgtccgc cgggaaaatc gcgatcggcg tagacctcgg gctcgaacca
     2761 gtccgcccgc gacgtcccgg tgtactgctc cagggcgtcg ccgaactgcg tctcgagttt
     2821 ttccacggtg gccaggctgg tgtcgcagcc gaagcggcag cacgagtcgt ggcagaactc
     2881 gcaatccatg cagtacgcga agaacttgcg gaggaaaatc agcggatcga cgtgcgtgac
     2941 gacgggcgcg ccgaaccgcg cgacgtatcg ccgcgagagc ggcacccgcc acgtgctcag
     3001 cactttgcga ttttccagaa cgatatcggg catcagttgc tccctgccaa agtggcctga
     3061 ttcatcgtca ccgggacagt cccgttcgtc gcggctctgg gcctatgcgc gcatacgcgg
     3121 gagaaaattt cgcacgcaga ttgtgtcaca actccttgcg ccgtcgtgat ttgcggcgaa
     3181 gaatctgcga tttgtattat gcgctttatg cgcgccagcg ccgcaacccg cgccctcccg
     3241 ccggaggtaa gccgcgacca ctggtcgcag ctgtgggcgc atgcgcgcat atgcgggaaa
     3301 ttgtttttgc acgcggttat tgtcgtaact ccttactcca tagtcattta cggctacaaa
     3361 tctgggttct gcattatgcg ctttatgcgc gccggcg
//
LOCUS       DASRJB010000907         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2792942, whole genome shotgun sequence.
ACCESSION   DASRJB010000907 DASRJB010000000
VERSION     DASRJB010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792942"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2210
                     /locus_tag="VFB80_23915"
     CDS             <1..>2210
                     /locus_tag="VFB80_23915"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZN36906.1"
                     /translation="RDAAATHSALDSFILARLEKEGLSPSPQASPLVLLRRASLALTG
                     LPPAPDEIETFLADRSPDAYERQVDRLLASPRYGERMAVPWLDAARYADTCGYQSDGE
                     RIMWRWRDWVIEALNANQPFDRFTIEQIAGDLLPRATLDQRLATGFHRNHRGNAEGGI
                     IPEEYAVEYVADRVETTATVWLGLTLGCCRCHDHKYDPFSQRDFYSLFAFFNNVPEKG
                     RAIKIGNSPPYIQTPTRPQQEKLAALSRQAEEAEGRWQDAASNLLPRERDTWLRTFAP
                     AAGFDWLPTTDLVAAVSLDGDSSERLSGKSGTWAGEEAAYEPGKLREAARFDGQSCLE
                     VGDVANFGFFDRFTLAAWVQFPAGGGGTIVSRMTDVEQGDGYQLAIAGGKLQLNFVKR
                     WLDDALRVETEALLAPGRWHHVMASYDGSRVAEGVRLFVDGRPAAVSVLLDELNQTFT
                     TKQPLRIGGGGGPAARFRGLIDEVRVYNDFSDELPALAVPKTIAELATSKLTPAEDAK
                     LTSYFLSEHASAELRAAYRRRLETARALRDFDVSLPTTMIMEEMPTPRPAHILIRGQY
                     DKPGQRVSPATPGELPPLAGGAPRNRLGLARWLVDPANPLTSRVAVNRAWQLHFGVGL
                     VKTAEDFGRQGEWPSHPELLDWLATEFMERGWDQKQLHRTIVTSGTFRQSSQVAAASL
                     ARDPDNRLLSRAPRLRLPAEMVRDQALFAGGQLLEQIGGPSVKPPQPAGLWSELT"
BASE COUNT          358 a          749 c          751 g          352 t
ORIGIN      
        1 cgcgacgctg ctgcgacaca ctccgcgctc gattcgttca tcctggcccg gctagagaaa
       61 gaagggcttt caccatcgcc gcaggccagc ccgctggtgc tcctccgccg cgcgtcgctg
      121 gccctgacag gtctaccgcc agcaccggat gaaatcgaga cgtttctcgc cgatcgctcg
      181 cccgacgcct acgagcgcca agtcgatcgg ctgctggcca gcccccgcta tggcgagcgg
      241 atggccgtgc cctggctcga tgccgcccgc tatgccgaca cctgcggcta tcagagcgac
      301 ggtgagcgga tcatgtggcg gtggcgcgac tgggtgatcg aggcgctcaa cgctaaccag
      361 ccgtttgacc ggttcacgat cgagcaaatc gccggtgacc tgctgccccg cgcgacgctc
      421 gaccagcggc tggccaccgg atttcaccgc aatcaccgcg gtaacgccga agggggcatc
      481 atcccggagg agtacgccgt cgaatacgtc gccgaccgcg tcgagacgac cgccaccgtc
      541 tggctgggcc tcacgctcgg ctgctgccgc tgccacgacc acaagtacga cccgttctcg
      601 cagcgcgatt tctacagcct gtttgcgttc ttcaacaacg tgcccgagaa gggccgggcg
      661 atcaagatcg gcaactcgcc gccctatatc caaacgccga cgcgcccgca gcaggagaag
      721 ctcgcggcgc tgtcgcgcca ggccgaagag gcggaagggc gctggcaaga tgccgcgagt
      781 aatctgctgc cacgggagag agacacctgg ttgcggacgt tcgcgcccgc cgccggcttc
      841 gattggcttc ccactaccga tttggtcgcg gccgtgtcac tcgacggcga ttccagcgag
      901 cggttgtccg gcaagtccgg cacatgggct ggcgaagagg ccgcgtatga gcccggcaaa
      961 ctccgagagg cggctcgttt tgacggtcag tcgtgcctgg aggtcggcga cgtcgccaac
     1021 ttcggcttct tcgatcgttt cacactcgcg gcctgggtgc agttccccgc gggcggcggc
     1081 ggcacgatcg tgtcgcgcat gaccgacgtg gagcaaggcg acggctatca gctggccatc
     1141 gccggcggca agctgcagct aaacttcgtc aagcgttggc tcgacgacgc cctccgcgtg
     1201 gaaacggagg ccctgctcgc gccgggccgg tggcaccacg tcatggccag ctacgacggt
     1261 tcgcgcgtcg ccgagggcgt gcggctgttt gtcgatggcc ggcctgcggc ggtgagcgtg
     1321 ctgctcgacg agctcaacca gaccttcacg acgaagcagc cgctgcgcat cggcggcggc
     1381 ggtggcccgg cggcgcggtt tcgcggcctg atcgacgaag ttcgcgtcta caacgatttc
     1441 tcggacgagc tgccggcgct ggccgtgcct aagacaattg ccgagctcgc cacgagcaaa
     1501 ctcacgccgg cggaggacgc gaagctgacc tcgtatttcc tgagcgagca cgcctccgcg
     1561 gagctgcgag cggcgtatcg ccggcggctg gaaacggcac gggcgctgcg cgatttcgac
     1621 gtcagcctgc ctacgacgat gatcatggag gaaatgccga cgccgcggcc ggcgcacatc
     1681 ctgatccgcg ggcaatacga caagccgggc cagcgcgtca gtccggcaac tccgggcgaa
     1741 ttgccgccgc tggcgggcgg tgcgccgcgg aaccggttgg gcttggcccg ctggctggtc
     1801 gatccggcca acccgctcac cagccgcgtg gcggtgaacc gggcctggca attgcacttt
     1861 ggcgtcggcc tggtgaagac ggccgaggat tttggccggc aaggcgagtg gccctcgcat
     1921 ccggagctgc tcgactggct cgctacggag ttcatggagc gcggctggga ccagaagcaa
     1981 ttgcaccgca cgatcgtgac cagcggcacc ttccgccaat cgtcgcaggt cgcggcggct
     2041 tcgctggctc gagatccgga caaccgactt ctttcccgag cgcccaggct gcggctgccg
     2101 gccgagatgg tccgcgacca ggcgctgttt gccggcgggc agctgctgga gcaaatcggc
     2161 ggcccctcgg tgaagccgcc gcagccggcg ggcttgtgga gcgagctgac
//
LOCUS       DASRJB010000908         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4204920, whole genome shotgun sequence.
ACCESSION   DASRJB010000908 DASRJB010000000
VERSION     DASRJB010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204920"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2261)
                     /locus_tag="VFB80_23920"
     CDS             complement(66..2261)
                     /locus_tag="VFB80_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN36907.1"
                     /translation="MIRLLSPRVLFLGAILAWLGLAEVALAGNKGPRRTSSTSSPAAD
                     GAAPSMPLTYPVTSRIQHVDDYHGAKIADPYRWLEDLDSNETRAWVEAQNKVTFAWLA
                     QLPHREPLRKRLTELWNYERFGLPVKRGGRYFFTRNDGLQNQNSIYVTESLDAPPRLL
                     IDPNQLSADGTIALTNWEVSDDGKLLAYDLAEAGSDWRECHVLEVETGRKRSDHLKWT
                     KFSRLSWTPDNKGFYYSRYDEPAAGQKYTGANYYQKLFYHRLGEPQSSDTLVYKRDDE
                     KEWGFEGQVTDDGRYLIISVWRGTEQKNQVFYQDLQAGGPVVELLAGFDAQYEFIDND
                     GPTFWFVTDAGAPLRRVIAIDTRTPGREQWKELIPESADTLVEASAVGDRFVATYLHD
                     ACSAVRIFDLAGKHVRDVELPGLGTVAGFRGRRTDRETFYSFTSYTDPGSIFRYDVQS
                     GETTLFRRPAVKFDASNYETRQVFYSSKDGTRVPLMIVSRKDLPLDGKQPTILYAYGG
                     FNIPQTPSFSVSTISWLELGGVYAVANLRGGGEYGRAWHEAGMLDKKQNVFDDFLAAA
                     EWLIEKKYTSSDRLAIRGGSNGGLLVGAAMTQRPELFAAAVPAVGVMDMLRFHKFTIG
                     WAWVSEYGSSDDPRQFQTLRTYSPLHNLKPGGKYPATLITTGDHDDRVVPAHSFKFAA
                     ALQAAQGGPEPVLIRIETRAGHGAGKSVEGKISEATDLWAFALAALGES"
     gene            complement(2319..2846)
                     /locus_tag="VFB80_23925"
     CDS             complement(2319..2846)
                     /locus_tag="VFB80_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36908.1"
                     /translation="MPSSVRPPRSPPPPAPRDINVRILVAPDELTWRDWPLFDRPVGS
                     LVAIGLAAAISCLAGWAAGSPLVGGAVAVFLLLLLWQTWLPTHYELSGSGITQSIIGW
                     RRRVPWTAIQNHEVKSDGVLLVPDAALTPLSPLRGLYLHCGSHKSAVLSHLDYYLRPW
                     NAASRTNSDRIEPLP"
     gene            2994..3272
                     /locus_tag="VFB80_23930"
     CDS             2994..3272
                     /locus_tag="VFB80_23930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36909.1"
                     /translation="MHQQSSASEPVDSGPDVPPQWRKAVDNPWLMLLVLFFVTAFLGL
                     PFLWMSRGFTTFWKVVVTIAVLIWTVLVFWVFWLIMVWCYTRIADALW"
     gene            complement(3332..4096)
                     /locus_tag="VFB80_23935"
     CDS             complement(3332..4096)
                     /locus_tag="VFB80_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36910.1"
                     /translation="MRLASCAFSLIAFALVCIPLPQASAADPTPTRSAGEGVNRDALD
                     LFDAISAEKIDAKLILKDETAGRLVVTNKTRQPLTIRLPEAFAGVPVLAQFNFPNNFP
                     NNNNNSRNSNNSNQSVGGGTNGYGNNGGLMSIEPEKAVKIKVVAVCLEHGKREPNQYV
                     AYEIEPLDQYTSDARLTEVVRALGRGDLDQKSAQAAAWHLANGLSWKQLAAKIGVRHI
                     GGRTELFFTTAQLQRAQAAISDASRRAEQLTSAGQP"
     gene            complement(4230..5360)
                     /locus_tag="VFB80_23940"
     CDS             complement(4230..5360)
                     /locus_tag="VFB80_23940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN36911.1"
                     /translation="MPSLPHARPAELGLAEHRLQSAYDRLDEWTSGKEPPVPGGAILV
                     GRHGKTLEPKLFGRQGPEAGAPPIRRDAIFLLASITKPVVYAGALMLVERGLLNLTDP
                     VTRYIPDFAAHHKEGTLVQHLFTHTSGLPDMLENDQQLRASHAPLEKFIEGAIKDTVP
                     LFPAGTSWSYQSMGTLVVAELIQRISGQRIHDYLQRQIIEPLGLKSTRLGSKGLDESR
                     LVRVQTPAWHGADFGWNSKYWRELGAPWGSMFSTPEDFAVICQLMLNGGEVGGVRLLS
                     PNTVRMMTSNRLDDYPLLPEPIRRTAPWGLGWKLNAPGTSGALCDLLGRQAFGHTGST
                     GNQLWMDPATGGFCILLTTAERARAPWRLAHLSSAVAAAFSA"
BASE COUNT          895 a         1696 c         1852 g          980 t
ORIGIN      
        1 gacgcggccg gagaggtcga aggcctccgc gcctcccggc tcgggggccc ccagctgagg
       61 ggccatcagc tctcgcccag cgcggccagc gcgaacgccc acaggtcggt ggcctcggag
      121 atcttgccct ccaccgactt gccggcgccg tgcccggcgc gcgtctcgat gcggatgagc
      181 acgggctcgg gcccgccctg cgctgcctgc agcgcggccg cgaacttgaa gctgtgggcc
      241 ggcacgacgc ggtcgtcgtg gtcccccgtg gtgatgagcg tcgccggata tttgccgccg
      301 ggcttgaggt tgtgcagcgg cgaatacgtc cgcaacgtct ggaactgccg cggatcgtcg
      361 gacgagccgt actcgctgac ccaggcccag ccgatcgtga atttgtggaa ccgcagcatg
      421 tccatcacgc cgaccgccgg caccgccgcc gcaaacagct ccggccgctg cgtcatcgcc
      481 gcgccgacca acaggccgcc gttagagccg ccgcggatcg ccagccgatc gctcgacgtg
      541 tacttcttct cgatcagcca ctcggcggcg gcgagaaaat cgtcgaagac gttctgcttt
      601 ttgtcgagca tgcccgcttc gtgccaggcc cggccgtatt cgccgccgcc gcggaggttc
      661 gccaccgcgt acacgccgcc caattccagc cacgagatcg tgctcaccga aaagctcggt
      721 gtctgcggaa tgttgaaccc gccgtaggcg tacaaaatcg tcggctgttt tccgtcgagc
      781 ggcaggtcct tgcgactgac gatcatcagc gggacgcgcg tgccgtcttt gctggaatag
      841 aagacctgcc gcgtttcgta gttcgaggca tcgaacttga ctgccggccg gcgaaacagc
      901 gtggtctcgc cgctctgcac gtcgtagcga aagatcgagc ccggatcggt gtagctggtg
      961 aacgagtaaa acgtctcgcg gtccgtccgc cggccgcgga agccggcgac cgtgcccagg
     1021 cccggcaact ctacgtcgcg gacgtgcttg ccggcgaggt cgaagatgcg gaccgcggag
     1081 caggcgtcgt gcagataggt ggcgacaaat cggtcgccga cggcgctggc ttccacgagc
     1141 gtgtcggccg attcgggaat cagttccttc cactgctcgc ggccgggagt gcgggtgtcg
     1201 atggcgatca cccgccgcaa gggagcaccg gcatcggtga cgaaccagaa cgtcggcccg
     1261 tcgttgtcga tgaactcata ctgggcatcg aacccggcga gcaattcgac gaccggcccg
     1321 cctgcctgga ggtcctggta gaagacctgg ttcttctgct ccgtgccgcg ccacaccgag
     1381 atgatgagat agcggccgtc gtcggtcacc tggccttcaa agccccactc cttctcgtcg
     1441 tcgcgcttgt aaaccagcgt gtcgctcgac tgcggctcgc cgagccggtg atagaagagc
     1501 ttctggtaat agttcgcccc cgtgtatttc tgcccggccg ccggctcgtc gtagcggctg
     1561 taatagaagc ctttgttgtc gggcgtccag gagagccgcg agaacttcgt ccacttcaga
     1621 tggtcgctgc gcttgcggcc ggtctcgact tcgagcacgt ggcactcccg ccagtccgag
     1681 cccgcctcgg ccaggtcgta agccagcagc ttgccgtcgt cgctgacttc ccagttggtc
     1741 agcgcgatcg tgccgtcggc gctgagctgg ttgggatcga tcagcagccg cggcggcgca
     1801 tcgagggatt ccgtaacgta aatcgagttc tggttttgca ggccgtcgtt gcgggtgaag
     1861 aagtaacggc cgccccgttt gactggcagc ccgaaccgct cgtaattcca gagctcggtg
     1921 agccgcttgc ggagcggctc gcggtgtggc agttgggcga gccaggcgaa cgtgactttg
     1981 ttctgggcct cgacccaggc ccgcgtctcg ttgctgtcca gatcttcgag ccagcgataa
     2041 ggatcggcga tcttggcgcc gtgatagtca tcgacgtgct gaatgcggct ggtgacggga
     2101 taggtcagcg gcatggaagg ggccgcgccg tccgcggcag gagaagaagt cgaggaagtg
     2161 cggcgcgggc ccttgttgcc ggccagagcg acctcggcca atccgagcca cgccagaatc
     2221 gcgccgagaa acagtacgcg cggagaaagg agcctgatca tacggtcacg gaagttgcat
     2281 caggggagag acgtaagaat gtgccaggaa agtttagcct atggcagggg ctcgatccta
     2341 tcgctattcg tgcggctggc cgcattccag ggccgcaggt aatagtcgag gtgcgacaga
     2401 acggcggact tgtggcttcc gcagtgcaga tacagcccgc ggagcgggga gagaggcgtc
     2461 aaggccgcgt cgggcacgag cagcacgccg tcgctcttga cttcgtggtt ctgaatcgcg
     2521 gtccatggca ctcggcgtcg ccagccaatg atcgactgcg tgatgccgct gccgcttagc
     2581 tcgtagtgcg ttggcagcca ggtctgccac aggagtagca gcaggaacac ggcgaccgcc
     2641 ccgcccacga ggggcgaacc cgccgcccag ccggcaaggc aactgatcgc cgccgccagc
     2701 ccgatcgcca ccagcgaacc caccggccga tcaaatagcg gccaatcgcg ccaggtcagc
     2761 tcgtccggcg cgacgagaat ccgcacattg atgtcgcgcg gtgcgggtgg cggcgggctg
     2821 cgcggcggac ggacggagga gggcatgact tcaaagtcga acctcgtcta gctcgcagcg
     2881 gaggcgcggg cgttttgcag tagccgccgt cgcctccgtc tccgggttaa acgacatata
     2941 cggtcaagtt cacctccaat atactttgcc agattcttcc gcgcgaaaag atcatgcacc
     3001 agcagtcgtc agccagcgag ccggtcgatt ccgggcccga cgttccgccc cagtggcgga
     3061 aggcggtgga caatccgtgg ttgatgctgc tggtgctgtt cttcgtcacg gcgtttctgg
     3121 ggcttccgtt cctgtggatg agccgcgggt tcacgacgtt ttggaaagtc gtcgtgacga
     3181 tcgcggtcct gatctggacc gtgctggtct tctgggtgtt ctggctgatt atggtttggt
     3241 gctacacgcg gattgccgac gcgctctggt gatatctggt gatagtagtc ctcacgctag
     3301 gcgtgaggag tgtcggtggg cgcagcccac cctacggctg gccagccgaa gtgagctgct
     3361 cggcacgccg cgaggcgtcg ctgatggcgg cctgggcgcg ctggagctga gccgtggtaa
     3421 agaacagctc ggtccggccg ccgatgtgcc gcacaccgat cttggctgcg agctgcttcc
     3481 aagagaggcc gttggccaga tgccacgcgg ccgcctgcgc cgacttttga tcgaggtcgc
     3541 cgcggcccag ggcgcggacg acttcggtca gccgcgcgtc ggacgtgtac tgatccagcg
     3601 gctcaatctc gtaggcgaca tactggttcg gctcccgctt gccgtgctcc aggcagacgg
     3661 cgacgacctt gatcttgacg gccttctcag gctcgatgct catgagtccg ccattgttgc
     3721 cgtagccgtt ggtcccgccg ccgaccgact ggttggaatt gttactgttg cggctgttgt
     3781 tgttattgtt cgggaagttg ttgggaaagt tgaactgagc cagcaccggc acgccggcga
     3841 aagcctcggg cagccggatc gtcagcggct gcctggtctt gttggtcacc acgaggcggc
     3901 cggcggtttc gtctttgagg atcagtttgg cgtcgatctt ttcggcggag atcgcgtcga
     3961 agaggtcgag ggcgtcgcga ttgactccct cgccggcgct tcgggttggt gtggggtcgg
     4021 ccgcggatgc ctgtggcagg ggaatacaga ccagcgcaaa agcgatcagc gaaaacgcac
     4081 agctagcgag tctcatggcg aaagtctccg cggtcagctt gctgccaaac cccgaaagca
     4141 ccggcaaatg ggcaaatccc acggccggcg ccgtcccctg attgcggagc ggctcgcacc
     4201 gacgggtgct ctagcagcag ttggcgtgcc taggcgctga aagccgcagc aaccgcgctg
     4261 gacaggtgag ccagacgcca gggggcgcgg gcccgctcgg cggtggtcag gaggatgcag
     4321 aagccaccgg tcgcggggtc catccacagt tggttgccgg tcgagccggt atggccgaag
     4381 gcctggcggc cgagcaggtc gcacagtgcg ccgctggtgc cgggggcgtt gagcttccag
     4441 cccaggcccc acggtgcggt gcggcggatc ggctcgggca gcaggggata atcgtccaag
     4501 cggttgctcg tcatcatccg cacggtattc ggcgagagca gccgcacgcc accgacttcg
     4561 ccgccattga gcatcagttg gcagatgacg gcaaagtcct cgggcgtgct gaacatcgag
     4621 ccccacggcg caccgagctc gcgccagtat ttgctgttcc agccgaaatc ggccccgtgc
     4681 caggcgggag tctggacgcg gacgagccgc gattcgtcga gtcctttgga gcccagccgc
     4741 gtcgacttca ggccgagcgg ctcgatgatc tgccgctgga gatagtcgtg aattcgctgt
     4801 ccgctaatcc gctggatgag ctcggcgacc acgagcgtgc ccatgctctg gtagctccag
     4861 ctggtgccgg ccggaaacag cggcacggtg tctttgatcg cgccttcgat aaacttctcc
     4921 agcggggcgt ggctggcgcg cagctgctgg tcgttctcga gcatgtcggg caggcccgag
     4981 gtgtgcgtga agaggtgctg gaccagcgtg ccttccttgt ggtgtgcggc gaagtcgggg
     5041 atgtaccgcg tcacgggatc ggtcaggttg agcagcccgc gctcgaccag catcagcgcg
     5101 ccggcataca cgaccggctt ggtgatcgac gccagcagga agatcgcgtc gcggcggatc
     5161 ggcggcgcgc cggcttcggg tccctggcgg ccaaacagct tgggctcgag cgtcttgccg
     5221 tgccgcccga cgaggatcgc tccgcccggc accggcggct ccttgccgct cgtccattcg
     5281 tcaaggcgat cgtaggccga ctgcaggcgg tgctcggcga ggccaagttc cgcggggcga
     5341 gcgtggggca gcgaaggcat agcgggttta ctccaagagg ctcgcccctc agattagcca
     5401 ccgcattctg gcgaaaccag cag
//
LOCUS       DASRJB010000909         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_561565, whole genome shotgun sequence.
ACCESSION   DASRJB010000909 DASRJB010000000
VERSION     DASRJB010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561565"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFB80_23945"
     CDS             complement(<1..551)
                     /locus_tag="VFB80_23945"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN36912.1"
                     /translation="MCPILLQAILALASAAPTDRPNVVLIVIDDLGWADLSCYGSKLH
                     RSPQIDKLAAGGVRFTQAYSACPVCSPSRAAIMTGRWPARLRLTDWLPGRGDLPAQRM
                     ARPKIRQELPLEEITIAEMFQDAGYATASIGKWHLGGTGFGPREQGFELNIAGDSGGS
                     PPGFFAPWTKTNAKGKTTGRQLVG"
     gene            661..2217
                     /locus_tag="VFB80_23950"
     CDS             661..2217
                     /locus_tag="VFB80_23950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZN36913.1"
                     /translation="MSSLLLTLALFAADPVQADLVLRGGTLYDGSGGEAQVGDVAVKG
                     DKIVAVGTFEVAGSPRIIDCKGRVVAPGFIDLHSHSDSGIVQAKTRQNANFLMQGCTT
                     VVTGNCGGGPTNVGEYFSKIDESGAGTNVLHLLPQGSLRSRVMGDANRPATAAELEEM
                     KTLADKAMREGACGMATGLIYVPGSFTKTDELIAIAEVVAKYHGFYASHIRNEGSQLL
                     ESLDEILTIGKQAKLPVHVSHIKVSGRSSWGLAPDAIRKLNEARAAGQQVTADQYPYA
                     ASSTSLSATVIPDELRSWTKLKAALDDPEQGHGAKVRQQIERSIQQKDGGGAVMIASF
                     SAERSWQGKNIAQIAKETSQTPLDVVLDIMRRGGAQIVNFGMNEDEVRLFMREPWVAT
                     ASDGSTQPLDSPAQPHPRAFGTFPRKIGRYAIEGGFVPLAQAIRSASGLPADVLRLTD
                     RGYLKPGYFADVVVFDPATFRDVATFEKPLVWATGTKLVLVNGQIAVENEKPTDKLAG
                     RALRHPVKQP"
BASE COUNT          414 a          791 c          744 g          337 t
ORIGIN      
        1 ccgaccagct gccggcccgt cgtcttgcct ttggcgttgg tcttagtcca gggcgcgaag
       61 aagcccggcg ggctgccgcc gctgtcgccg gcgatgttca gttcgaaacc ttgctcgcgc
      121 ggaccgaagc cggttccgcc caggtgccac ttgccgatgc tcgcggtcgc atagccggcg
      181 tcctggaaca tttccgctat cgtgatctcc tccagcggga gttcctgtcg gatttttggc
      241 cgggccattc gctgagccgg caggtcgccg cggcccggca gccaatcggt caggcgcaac
      301 ctcgccggcc agcggccggt catgatcgcc gcccgggagg gcgagcagac cgggcaggcg
      361 gaatacgcct gcgtaaaccg cacgccgccc gccgccagct tgtcgatctg cggcgagcgg
      421 tgcagcttgc tgccgtagca gctaaggtcc gcccagccaa ggtcgtcgat cacgatcagg
      481 accacgttgg ggcggtcggt gggcgccgcg gaagccagcg cgagaatcgc ttggaggagg
      541 atgggacaca tgggactgag gggacgaatg agccggaaat tgaatcggtt ctgacgctcc
      601 gttatacatg gttctgccaa ccgactcacc gaccgaccga ccaccgacca accgactccg
      661 atgtcctcgc tgctcctcac cctcgcgctc ttcgccgccg atcccgttca ggccgatctc
      721 gtgctccggg gcggcacgct ctatgacggc agcggcggcg aggcgcaagt cggcgatgtg
      781 gccgtgaagg gtgacaagat cgtggcggtg ggcacgttcg aagtggcggg gtcgccgcgg
      841 atcatcgact gcaagggccg cgtcgtcgcg ccgggcttca tcgacctgca ctcgcacagc
      901 gacagcggca tcgtgcaggc caagacgcgg cagaacgcca acttcctgat gcagggctgc
      961 acgaccgtcg tcaccggcaa ttgcggcggc gggcccacga acgtcggcga atacttcagc
     1021 aaaatcgacg aaagcggcgc gggcaccaac gtgctgcacc tcctgccgca gggcagcctc
     1081 cgcagccggg tgatgggcga cgccaaccgc ccggcgaccg cggccgaact ggaggaaatg
     1141 aaaacgctgg ccgacaaggc catgcgcgag ggcgcctgtg gcatggcgac gggcctcatc
     1201 tacgtccccg gatcgttcac caaaaccgac gagctgatcg ccatcgccga ggtcgtcgcg
     1261 aaataccacg gcttctacgc ctcgcacatc cgcaacgaag gctcgcagct cctcgaatcg
     1321 ctcgatgaaa tcctgacgat cggcaagcag gcgaagctgc ccgtgcacgt ctcgcacatc
     1381 aaggtcagcg gacgctcaag ctggggcctg gcccccgacg cgatccgcaa gctcaacgag
     1441 gcccgcgccg ccggccagca ggtcaccgcc gaccagtatc cctatgccgc cagcagcacg
     1501 tcgctctcgg ccaccgttat ccccgacgag ctgcggtcgt ggaccaaact caaggcggcc
     1561 ctcgacgacc cggagcaggg acacggcgcg aaagtgcggc agcagatcga gcgctcgatc
     1621 cagcaaaagg acggcggcgg cgccgtgatg atcgcctcct tctccgccga gcgctcctgg
     1681 caagggaaaa acatcgccca gatcgccaag gagacgagtc agacgccgct cgacgtcgtc
     1741 ctcgacatca tgcgccgcgg cggggcgcag atcgtcaact tcggaatgaa cgaggacgaa
     1801 gtacggctgt tcatgcgcga gccctgggtg gcgacggcga gcgacggcag cacgcagccg
     1861 ctggattcgc cggcgcagcc gcacccgcgg gcttttggga cgtttccacg gaaaatcggc
     1921 cgctacgcga tcgagggagg cttcgtcccg ctggcccagg caatccgcag cgccagcggc
     1981 ctgcccgccg acgtgctccg cctgaccgac cgcggctact tgaagccggg ctattttgcc
     2041 gatgttgtcg tgttcgaccc cgccacgttt cgcgacgtgg ccacgtttga gaagccgctg
     2101 gtgtgggcga ccggcacgaa gctggtcctc gtcaacggcc aaatcgctgt cgagaacgag
     2161 aagcccaccg acaagctcgc cggccgcgcg ctccggcacc cggtgaagca gccctgatcc
     2221 caaagccgcg accagtggtc gcgctctacc cggtgatcca tagccacatc gcgccgaacg
     2281 ccggcc
//
LOCUS       DASRJB010000910         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3937380, whole genome shotgun sequence.
ACCESSION   DASRJB010000910 DASRJB010000000
VERSION     DASRJB010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3937380"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFB80_23955"
     CDS             <1..289
                     /locus_tag="VFB80_23955"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN36914.1"
                     /translation="RLRRELLTGVLLGMACGLILALVALAWQGKAAVALSLIAGIGLG
                     IAGSALFGFAMPYALRLAQRDPQVAAGPIALVFADLLTLTIYFGVAHWLMG"
     gene            complement(298..2097)
                     /locus_tag="VFB80_23960"
     CDS             complement(298..2097)
                     /locus_tag="VFB80_23960"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36915.1"
                     /translation="MSHSLLAQRGAVLDGAVESAQLRPAHQSYHRRGLAARSLSANDL
                     AAWRIPPEVLESVPESVARENLVCPLGDFGGRLIFAAADPEDLSIADKLRFILNRDVA
                     LIGAPRHAVLQAIDSNYEDIEGESADSVLQEFTDTAIDFTETESTGVYLADAMPLMPT
                     PRARRKCGRLPDSGQRARGAGMFYQTVEEGQRILMKRLDGTMEVLVGPRRVWRGWRRF
                     ESMSHHVAHPGEFLSIRYRDGRQEHVAGPAELWFDPRTHEEIDVREALQLSAQEAVVV
                     YSRPAASDGTKPAAAARRIVYGPGLFVPEPGEWLHTFSWHASKGGSRGVEKVPNALVF
                     QKLWLMPDQMYHDVHDVRTADDAVLTIRLMIFFELVDISRMLDATHDPIGDFVNAATA
                     DVVEFTGRHEFEAFKRNTAALNDLATYRQLAGRAAQCGYRISNVVYRGYGAADSLQQM
                     HNQAIEARTKLQLDRATEQQAQELEDFRLASQLTRAAKRRSEQTAEVRHDLQLASEKH
                     DADLALRERQQSFQRQQRLADARQRDDIARATAQQQQEHFRTLKELGVDLTAFLTQSR
                     ADRVIELRGSLPAHVHLDRLDDSPANGHAATAD"
     gene            complement(2211..2582)
                     /locus_tag="VFB80_23965"
     CDS             complement(2211..2582)
                     /locus_tag="VFB80_23965"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZN36916.1"
                     /translation="MSECSGFGVQGSAASGGRKPTEAGLVVRVPRGIRSKEKLLAILA
                     DKLRFPHYFGHNWDALEECLRDLSWLPAGQPVEIVHDDLPFGAGGENRGTYLDIVTKA
                     AAAQPGRLRVILPEQAKTREP"
BASE COUNT          401 a          905 c          899 g          467 t
ORIGIN      
        1 gcggctgcgg cgcgagctcc tgaccggggt gctgctgggc atggcctgcg gcttgatcct
       61 ggcgctcgtc gcgctcgcct ggcaaggcaa ggcggccgtc gccctgagcc tgatcgccgg
      121 catcggcctg ggcatcgccg gctcggcact gttcgggttc gcgatgccct acgccctgcg
      181 gctggcccag cgcgacccgc aagttgccgc cggacccatc gccctggtct tcgccgacct
      241 gctcacgctc acgatctatt tcggcgtcgc ccactggctg atggggtaag tacgccttca
      301 gtctgctgtc gccgcgtggc cgttcgcagg cgagtcgtcc agccggtcca ggtgcacgtg
      361 cgccggcagc gaaccgcgga gctcgatcac gcggtcggcg cgagattgcg tgaggaaggc
      421 ggtgagatcg acgccgagct ccttgagcgt gcggaagtgc tcctgctgct gctgggccgt
      481 ggcccgggcg atgtcatcgc gctggcgggc gtcggcgagg cgctgctggc gctggaagct
      541 ctgctgccgc tcgcggaggg ccaggtcggc gtcgtgcttt tcggaggcca actgcaaatc
      601 gtgccgtact tcggcggtct gctcgctgcg gcgcttggcg gcgcgggtca gctggctggc
      661 caggcgaaag tcctccagct cctgggcctg ctgctcggtg gcccggtcga gctggagctt
      721 ggtgcgggcc tcgatcgcct ggttgtgcat ctgctgcagc gagtcggccg cgccatagcc
      781 gcggtaaact acgttgctga tccggtagcc gcactgggcc gcccggccgg cgagctggcg
      841 ataggtcgcc aggtcgttga gcgcggcggt gttccgcttg aaggcttcga actcgtgccg
      901 gcccgtaaac tcgaccacgt cggcggtcgc cgcgttgacg aagtcgccga ttgggtcgtg
      961 agtggcgtcg agcatccggc tgatatcgac cagctcgaag aagatcatca agcggatggt
     1021 gagcacggcg tcgtcggcgg tccgcacgtc gtgcacgtcg tggtacatct gatcgggcat
     1081 cagccagagc ttttggaaga cgagtgcgtt gggcaccttt tcgacgccgc gcgagccgcc
     1141 cttgctggcg tgccacgaga acgtgtgcag ccactcgccc ggctccggca cgaacaggcc
     1201 cgggccatac acgatccgcc gcgctgccgc ggccggcttg gttccatcgc tcgccgccgg
     1261 ccgcgaatag acgacgactg cttcctgggc ggagagctgc aaggcttcgc ggacgtcgat
     1321 ctcttcgtgc gtccgcggat cgaaccagag ctccgccggc ccggcgacgt gctcctgccg
     1381 gccgtcgcgg tagcggatgc tgaggaactc gcccggatgg gcgacgtggt gcgacatgga
     1441 ctcgaaccgc cgccagccgc gccacacccg ccgcggcccg accagtacct ccatcgtgcc
     1501 gtccaagcgc ttcatcagga tccgctggcc ttcctcgaca gtttggtaga acattccagc
     1561 acctcttgct cgttgcccgc tgtctggcag acggccgcac ttgcgcctgg cccgcggcgt
     1621 gggcatcagc ggcatcgcgt ccgcaaggta cacgccggtg gattccgtct cggtaaagtc
     1681 gatcgccgtg tccgtgaact cttgcagcac cgagtcagcc gattcgccct cgatgtcctc
     1741 gtagttcgag tcgatggctt gcaacacggc gtggcgcggc gcgccgataa gcgctacgtc
     1801 gcggttgaga atgaaccgca gcttgtcggc gatggacagg tcttcgggat cggccgcggc
     1861 gaagatcagc cgcccgccga agtcgccgag cggacagacg aggttctccc gcgccaccga
     1921 ctccgggacg gactccagga cttccggggg aatccgccac gcggccagat cgttagctga
     1981 gaggctccgc gcggcaagcc cgcggcggtg gtacgactgg tgcgcgggcc gcaactgcgc
     2041 actttcaacg gccccatcga ggacggctcc tcgctgggcc aaaagcgaat ggctcactgc
     2101 cgatccacct ccgcccgaac gggcgaccta gctgtttgga tcagccccag ctggcctgtt
     2161 agacaagacg aacaccagcg cggaaatgct tcgcgtgtcc agtgaaaaag tcaaggctcg
     2221 cgcgttttcg cctgctcggg caagatcaca cgcaggcgtc cgggctgcgc ggcggccgct
     2281 ttggtcacaa tgtccagata cgtcccgcgg ttctcgccgc ctgcgccgaa gggcaggtcg
     2341 tcatgcacga tctcaaccgg ctgcccggct ggcagccacg acaaatcgcg cagacactct
     2401 tcgagcgcgt cccagttgtg cccgaaatag tgcgggaagc ggagcttgtc ggccaggatc
     2461 gccagcagct tctccttgct ccgaatgccg cgcggaaccc gcacgaccag gccggcctca
     2521 gtgggcttac gcccaccgct cgccgctgaa ccctgaaccc cgaaccctga acactcgctc
     2581 atggcgaatc cacgcgctga aacgtccgat aatggtcggg cgtgtaatac gtctcgccct
     2641 tttcgccgac gacgattcgc tgcggaccgg gg
//
LOCUS       DASRJB010000911         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_271948, whole genome shotgun sequence.
ACCESSION   DASRJB010000911 DASRJB010000000
VERSION     DASRJB010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271948"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFB80_23970"
     CDS             <1..544
                     /locus_tag="VFB80_23970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN36917.1"
                     /translation="LAAGRLDQASRMPDLVRAWRIVGSRRSEARLWIVGDGSLRDQLY
                     QQIGDLDQRFRVLIPGTFDCLQEVMQAADLFLAPAPQVVPPLALLEALAAGLPVLAAD
                     SPALRGLAAAHADAMLFPPGDVQRLAAAICDQFEHPAAGILRGSAIRQQLHAAGTPAD
                     EAAAYREVLSRAGAPHAGPP"
     gene            622..1725
                     /locus_tag="VFB80_23975"
     CDS             622..1725
                     /locus_tag="VFB80_23975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN36918.1"
                     /translation="MLRLLLVIPTLVRGGAEKQLTLLAAGLPRDQFDVHVAVLTHTGP
                     YEQTLREAGIPVTVVGKRWKVDPLCYWRLKRHIRTLKPDLVHTWLFAANAYGRHAAHA
                     AGVKHILAGERCVDPWKSALQLAIDRALAKRTERIVTNSSGVREFYVGRGLPAEKFVV
                     IPNGIAANGPAEPISREQLLTELKLPPNARLIGAVGRLWPQKRVKDLIWAADLLKTTR
                     DDTHLLIIGDGPQRWRLERYREQNRMIDRAHFLGERSDVPQILPHLDALWLGSEYEGQ
                     SNAIMEAMAAGVPVIATDIPGNRDLVVPDVTGYLVPIGERFEFARRTHWLLEDADLRK
                     RLGEAGRERIQSEFSVAQMVDRHAQLYRELAVR"
     gene            1811..3013
                     /locus_tag="VFB80_23980"
     CDS             1811..3013
                     /locus_tag="VFB80_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZN36919.1"
                     /translation="MPASASTALACAAALSICQRAGDALREAASAARESLGGTPDLAL
                     LFSSPHHSGEVEQLAADACELLGTRNLLGCTGESIVGTGQEIEDSPALSIWLARWPGV
                     RLTPLHLAFERTPEGGVLRGWPDELAGDWPSGSFLLCLGEPFTFPTDFLLERMNEDRP
                     GVPVVGGMASGAAQPGENRLILGQKIYAEGAVAVHVAGPIRLRSVVSQGCRPIGRPFV
                     VTKAERNMIFELGGKPALVQLREIFAALPTREQRLVQQALHVGRVVSEYQDRFEQGDF
                     LVRNVIGIDSNSGAIAIGDYLRPGQTVQFHIRDEEAADAELKQLLSKAKAASPAVPAG
                     GLLFTCNGRGTRMFSQPHHDAAAVARAFPALPLAGLFAAGEIGPIGRQNFVHGFTASL
                     ALFEPADG"
     gene            3158..>3679
                     /locus_tag="VFB80_23985"
     CDS             3158..>3679
                     /locus_tag="VFB80_23985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36920.1"
                     /translation="MPIKVTCQCGKSFAAKDELAGKAVKCPNCQQPLRIPGAAQPAAK
                     SIAQPAAKGNAQPAAAKVAAAAPAKAATKPASASSSGIGLGDEVRARSLDDSLLSEVG
                     LHQQVANTRPCPGCMAPLGPDAVVCIKCGYNTKLGRRMQTVSVTGGGGPEGHSAIAEE
                     LLQKAADSIEEQKD"
BASE COUNT          592 a         1262 c         1221 g          604 t
ORIGIN      
        1 cctggccgcc ggccggctcg atcaggccag ccgcatgccc gacctggtgc gggcctggcg
       61 gatcgtcgga tcgcgccgct ccgaggcgcg gctctggatc gtcggcgacg gaagcttgcg
      121 cgaccagctg taccagcaga tcggcgacct cgaccagcgg tttcgcgtac tgattcccgg
      181 gaccttcgat tgcctccagg aggtgatgca agccgccgac ttgttcttgg cgcccgcgcc
      241 gcaggtcgtg ccgccgctgg cgctgctgga agctctcgca gccgggttgc cggtgctcgc
      301 ggccgattcg ccggcgctgc gcggcctggc ggctgctcac gccgacgcga tgctttttcc
      361 gcccggcgac gtgcagcgcc tggcggcggc gatctgcgac cagttcgagc atcccgccgc
      421 cggcatcctc cgcggctcgg cgattcgcca gcaactgcac gccgctggga cacccgccga
      481 cgaggccgcc gcttatcgcg aggtgctttc gcgagcgggc gcccctcacg cgggaccgcc
      541 ctagatcgcc tgggcaatcg tcgtcccttg gatcaaactg taactcactc cgtattccga
      601 attccgcatt ccgtatttcc cgtgcttcgc ttgctactcg tcattccgac actcgtccgc
      661 ggcggcgcgg aaaagcagct cacgctgctg gcggcaggcc tgccccgcga tcagttcgac
      721 gtccacgtgg cggtgctgac gcataccggc ccctacgagc agacgctccg cgaagccggc
      781 attcccgtta cggtcgtcgg caagcgctgg aaagtcgatc cgctctgcta ctggaggctg
      841 aagcggcaca tccgaacact gaaacccgac ctcgttcaca cctggctctt cgccgctaat
      901 gcttacggac ggcacgcagc ccatgccgcc ggcgtgaaac acatcctggc cggcgagcgg
      961 tgcgtcgatc cctggaagtc ggctctgcag ctcgccattg accgggccct ggccaaacgc
     1021 accgagcgga tcgtcaccaa cagcagcggt gtgcgcgagt tctatgttgg ccgcgggctg
     1081 cccgccgaaa agttcgtcgt gattcccaac ggcatcgccg ccaatgggcc ggctgaaccg
     1141 atcagccgcg agcagctact taccgagctg aagctgcccc cgaatgcccg gctgatcggc
     1201 gcggtgggcc ggctctggcc gcagaaacgg gtcaaggact tgatctgggc ggcggacctg
     1261 ctcaagacca ctcgcgacga cacgcacttg ctcattattg gcgacgggcc gcagcgctgg
     1321 cggctggagc gttaccgcga gcagaaccgg atgatcgacc gggcccattt tctgggcgag
     1381 cggtccgacg tgccgcaaat cctgccgcat ctggacgcgc tctggctggg aagcgagtac
     1441 gaggggcagt cgaatgcgat catggaggca atggcagccg gcgtgcccgt gattgctacg
     1501 gacattcccg gcaaccgcga cctcgtggtg ccagatgtca ccggctacct ggtacccatc
     1561 ggcgaacgat ttgagtttgc ccgccgcacg cactggctgc tggaagatgc ggatctccgg
     1621 aagcgcctcg gcgaggccgg ccgtgaacgg attcaaagcg agtttagcgt cgcgcaaatg
     1681 gttgaccggc acgcacagct gtaccgcgag ctggccgtgc ggtgaaccgc acgctggccg
     1741 cgtagcacgc cgccgcggcg gcgactatca tgcgccaact tgtcgctagc gactctgggg
     1801 ccagccgctc atgcctgctt ctgcatccac ggcgcttgct tgcgccgccg cgctttcgat
     1861 atgccagcga gcaggcgacg cgctgcgcga agcggccagt gcggcccgcg aatcgctcgg
     1921 cggaactccc gacctggccc tgctcttcag ctcgccgcac cactccgggg aagtcgagca
     1981 gctagcagcc gacgcctgcg agctcttggg aacacgcaac ctgctgggct gcacaggcga
     2041 gtcgatcgtc ggcacggggc aggaaatcga ggattcgccc gcgctgtcaa tctggctggc
     2101 tcgctggccg ggcgtgcgtt tgacgccgct gcacctggcg ttcgaacgca cgccggaggg
     2161 cggcgtactc cgcggttggc cggacgagct ggccggcgac tggccgtccg gatcgttcct
     2221 cctttgcctg ggcgagccct tcacctttcc gaccgacttt ctgctggagc gcatgaacga
     2281 agaccgcccg ggcgtgcccg tcgtgggagg catggccagc ggcgcagcgc aacctggcga
     2341 gaatcggctc atcctcggcc agaaaatcta tgcggagggc gcggtcgccg tgcacgtcgc
     2401 cggccccatt cgcctgaggt cggtcgtttc gcaaggctgc cggccgatcg ggcggccctt
     2461 tgtggtcacc aaggctgagc gaaacatgat cttcgagctg gggggcaagc ccgcgctggt
     2521 gcaactccgc gaaatcttcg ccgcgctgcc gacccgcgag cagcggctgg tgcagcaggc
     2581 gctgcacgtc ggccgcgtcg tcagcgagta tcaggaccgg ttcgagcaag gcgattttct
     2641 tgtccgcaat gtgatcggca tcgattcgaa cagcggagcg attgccatcg gcgactatct
     2701 gcgccctggc cagaccgtgc agtttcacat ccgcgacgag gaagccgccg acgcggagct
     2761 gaagcagctc ctgagcaagg ccaaggcggc atcccctgct gtgccagcgg gcggactgct
     2821 cttcacctgc aacggccgcg gcacgcggat gttctcgcag ccgcaccatg atgccgccgc
     2881 ggtggcccgc gcctttcccg ccctgccgct cgccggactc tttgccgccg gcgaaatcgg
     2941 ccccatcggg cggcagaact tcgtccacgg cttcaccgcc agcctggccc tcttcgagcc
     3001 cgccgatggc tgacgctctg gcagcttggc cgcaaattaa cagcgccagc tctgcaaccg
     3061 gcctgttggc cggggttaag cggcacaacc gttgttaatc ctcggcttcg tgcgcaaaat
     3121 ggggcttgtt ctcgaccctt ctcccggcgg cgcggttatg ccgatcaaag tcacttgtca
     3181 gtgcggaaag agcttcgcgg ccaaggatga attggccggc aaggcggtga agtgccccaa
     3241 ctgccagcag ccgctgcgga ttccgggcgc cgcgcagccc gccgcaaaga gcattgcgca
     3301 gcccgccgcc aaaggcaatg cgcagcccgc cgcggcgaaa gtggccgctg ccgcgccggc
     3361 gaaggcggcg accaagccgg ccagcgcgtc gtcgtcggga atcggcctgg gggacgaagt
     3421 ccgggcgcgg agcctcgacg acagcctgct gagcgaggtc ggcctgcacc agcaggtggc
     3481 caacacgcgg ccctgccccg gctgcatggc gccgctcggt ccggacgccg tggtgtgcat
     3541 caagtgcggc tacaacacaa agctcggtcg gcggatgcag accgtttcgg tcaccggcgg
     3601 cggcgggccg gaagggcata gcgcgatcgc cgaagagttg ctgcaaaagg cggccgattc
     3661 gatcgaggag caaaaggat
//
LOCUS       DASRJB010000912         5970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1125616, whole genome shotgun sequence.
ACCESSION   DASRJB010000912 DASRJB010000000
VERSION     DASRJB010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125616"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFB80_23990"
     CDS             complement(<1..689)
                     /locus_tag="VFB80_23990"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HZN36921.1"
                     /translation="MRESPRSRRLKSDHKALTQLAAESTILEFTPYGTPPDFYILKFR
                     GRGIWKPEPQAEVQLREEHEVHIRLGASYPRMMPELAWKSPIFHPNISASGVVCLGGY
                     GTYWVPSLALDELCGMLWDMIRYENYDETSPYNREAAAWAKNQTYYPLPLDRRPLRDK
                     LAAVSAEENLLPIPLPKPTRPKSSSGMSPFRPAPVPQVRQALRPALPQPAASDVVFIG
                     EVVQAVQPRSAG"
     gene            complement(796..1083)
                     /locus_tag="VFB80_23995"
     CDS             complement(796..1083)
                     /locus_tag="VFB80_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HZN36922.1"
                     /translation="MSAGEERKGTRVTFLDQTGAKSVEAVIADTVQVKRILPNIITKM
                     NLPVMGPDGQPMSYSLDHKEGGKRLREDDTLPSSGVGDGHHLIVYPEIVAG"
     gene            complement(1284..2147)
                     /locus_tag="VFB80_24000"
     CDS             complement(1284..2147)
                     /locus_tag="VFB80_24000"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN36923.1"
                     /translation="MAKLIIGCGYLGLRVARLWVAAGERVFAVTRSAERGSQLAAAGI
                     EPLVADITADAQLQLPQDVRTVLFAVGHDRGSRQSIHQVYVGGLRTATDHLPEAVRKV
                     IYISTTGVYGQVTEGDVDEGSPCLPTREGGKASLAAEQLLQASRFGSRAIILRLAGIY
                     GPGRMPRSGDLLAGRPIDAPSQGWLNLIHVEDAARIVLLAEERADAPRTYVVSDGQPV
                     QRRDYYAELARQLKAPPPQFVAPPPDSPAQKRAASDKRVQPWRMFRELKPHLVYPNYR
                     EGLAAMVAESA"
     gene            2317..3399
                     /locus_tag="VFB80_24005"
     CDS             2317..3399
                     /locus_tag="VFB80_24005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN36924.1"
                     /translation="MRRGWPRSLYLGLAAAAAFSLAFSPPLMEHCGRAFAAEVAPADS
                     AATDVVAAALKTLFAGGNPAGVADLKAMQTHVQKLTEQLKKCTVGVTVGMAQGSGVIV
                     SKDGYVLTAAHVAGAPNSRVEFMLSDGKVVRGKSLGLFRTRDAGLMKITDPGDYPFAE
                     MGSSTELKNGQWCLALGHPGGYQEERGMVLRVGRVLLNDLTENVIVTDCTLVGGDSGG
                     PLFDMDGHVIGINSRIAESLTSNMHVPVSTFKETWDRLKNGEDWGHLPGQEPWLGVRG
                     DDKAKDALIAEVVPGSPADDAGIKKGDVIISYDGEYIPDFAALKAKVSSWRPTFGPRG
                     SRPSSTVQVLRGGEVVELRLRLRPRG"
     gene            3445..4497
                     /locus_tag="VFB80_24010"
     CDS             3445..4497
                     /locus_tag="VFB80_24010"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN36925.1"
                     /translation="MRKSAEAASRRLLPVAAVLLLLLPAARAQDRLHRRVLEAFRPVV
                     SEPSKSTTRIYCDGSQKAMGAVVQADGYIVTKASELSGNIEVQTHDIPRKLSARIVGT
                     DPTTDLALLKVEAKELPVLAWAGDPVPPIGSWLATPGLESSPVAIGILSVAPRKIPNG
                     GALGITMDRSQDVPRIVEIKPDSGAAKAGLQVGDIIRQVNKDVVKGQQNLRETIGKYQ
                     RGDKVTLVVERDGKELTVEATLGSMSQLIMGERAGFQNGLGGKLSDRREGFPLALQHD
                     TVLRPSDCGGPIVDLDGKAVGLNIARAGRVESYALPAALARESIGKLLAQANTMPADS
                     KLVDKQTPAEGEKKIQ"
     gene            4620..4892
                     /locus_tag="VFB80_24015"
     CDS             4620..4892
                     /locus_tag="VFB80_24015"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HZN36926.1"
                     /translation="MPDRLEKLRATLAELEAELRELESLDDATRDVLADAAAEIARTL
                     RRGKRSPETQQAESSLQGQIASFEASHPQLAGIVTRLIDGLGQLGI"
     gene            complement(4965..>5970)
                     /locus_tag="VFB80_24020"
     CDS             complement(4965..>5970)
                     /locus_tag="VFB80_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36927.1"
                     /translation="GGSIGKYTIDVLAAPTSSSGNSGGGGSSGTAGPVIVQGSIATIS
                     GTVAADVFVWNAALPQQITINGAAYALGGATQIRLEGKGGSDTLTITGGSVAETVRLR
                     PGSVEFVGGGFSLNATGVETARFYGGASDRAWLYDSAANDYLEAGPAFARLAGGGFES
                     AAFGCGAVTVIAGSGNDAAALRDSAGADLLDANPSYVWLRGAGFSIRAEGFDDVTVQA
                     TAGSGDAAGFYGSAAGDLLWVWGGTRNLYAGGVQIRTEGFQSAYFDGAGGQDQIDFNS
                     SGPQPRLRGRGSQGTIADGALATEFAHVESVLASVRKNHKLQTDLKAVDFVFRKIGGP
                     "
BASE COUNT         1006 a         1976 c         1988 g         1000 t
ORIGIN      
        1 cccgcgctcc gcggctgtac cgcctggacg acttcgccga tgaaaaccac gtcgctggcc
       61 gccggctgcg gcaaggcggg ccgcagcgct tggcggacct gtggcacggg cgccggccgg
      121 aagggcgaca tgccggatga ggacttgggc cgcgtcggct tgggcagcgg aatcggcagc
      181 aggttttcct cggccgagac cgcggcgagc ttgtcgcgca gcggccgccg gtccagcggc
      241 aggggatagt aggtctgatt cttggcccag gccgcggcct cgcggttata ggggctcgtc
      301 tcgtcgtagt tttcgtagcg gatcatgtcc cagagcatgc cgcacaattc gtcgagcgcg
      361 aggctgggca cccagtacgt cccatagccg ccgaggcaga cgacgccgct ggccgagatg
      421 ttggggtgga agatcggcga cttccacgcc agctcgggca tcatccgcgg atacgacgcg
      481 ccgaggcgaa tatgcacctc gtgctcctcg cgaagttgca cttcggcctg cggctcgggc
      541 ttccaaatgc cgcggccgcg gaacttcagg atgtagaagt ccggcggtgt gccgtagggc
      601 gtgaattcga ggatggtgct ctcggccgcc agctgcgtga gcgccttgtg atcgcttttc
      661 aggcggcgag agcggggcga ttcgcgcatg attcctccaa ggtagctccc tcacgccgtg
      721 agggagcagc agcgggcaac gctgtccata atccagcgct tcccgcctcg gcccctgacg
      781 gcgtcagggg cctacttagc ccgccacgat ctcgggatag acgatcaggt ggtgcccgtc
      841 gccgacgccc gacgacggca gcgtgtcatc ctcacggagc cgcttgccgc cctccttgtg
      901 gtcgaggctg tagctcatcg gctgaccgtc ggggcccatc acgggcaagt tcatcttcgt
      961 gataatgttg ggcaggatgc gtttgacctg gaccgtgtcg gcgatcaccg cttcgacgct
     1021 cttggcgccg gtctggtcca gaaacgtgac ccgcgtccct ttacgttctt cgccggccga
     1081 catgagcaga ctcctctgac tggcacgctt ctgcggcgga aacgccaccg gaatacaccg
     1141 cgagcgcgat tgaaacgatc ggttcgatga ggtattcgcc gggcgtctcc aaatcttaag
     1201 gcacggctgg ggcgattcct aggctcactc tcgcttagcc ccggccccca ggccgggtga
     1261 atcaaccggc atggacgccg gggctaagcg gactcagcca ccattgctgc tagcccctca
     1321 cgatagttgg gatacaccag gtgcggcttt agttcgcgga acatccgcca cggctgcacc
     1381 cgcttgtcgc tggcggcgcg cttctgcgcg ggcgaatcgg gcggcggcgc gacaaattgc
     1441 ggcggcgggg ccttgagctg gcgggccaac tcggcatagt agtcccgccg ctgcaccggc
     1501 tgcccgtccg aaacgacata cgttcgcggc gcgtccgccc gctcttcagc cagcagcacg
     1561 atccgcgccg catcttcgac gtggatcagg ttcagccagc cctgcgatgg cgcgtcgatt
     1621 ggccggccgg ctagcagatc gccgctccgg ggcattcggc cagggccata gatgcctgcc
     1681 agccgcagaa tgatcgcccg cgaaccgaat cggctggctt gcagcagctg ttcggcagcg
     1741 agggaagctt tgccgccttc gcgggttgga aggcagggcg agccttcgtc cacgtcgccc
     1801 tcggtaactt gtccgtaaac gccggtggtg ctgatgtaga tgactttcct gacagcttcc
     1861 ggcaggtggt cggtcgccgt cctcaggccg ccgacataga cctgatggat cgactggcgg
     1921 ctgcctcgat cgtggccgac ggcgaaaagc accgtccgaa catcttgcgg cagttgaagt
     1981 tgtgcatctg ccgtaatgtc cgcgaccagc ggctcgatgc ctgcggcggc caattgtgag
     2041 ccgcgctcgg ccgagcgggt gacggcaaaa acccgctcgc ccgccgcgac ccataggcgg
     2101 gcgactcgca gcccgaggta tccgcagccg atgataagtt ttgccatcga ggagaaatcg
     2161 ggaataattg cacaggccat gcagaataag tcccggtagc cgacgctgcc agcgtcggcc
     2221 cagaatctgg cgacaacctg cgagcaattc caaaaccttt cgggtaggat gttgttcaat
     2281 aagtccgtac ggccactggc tttcggaggg agagcaatgc gaagaggttg gccccgatcg
     2341 ctgtacttgg gacttgcggc ggcagccgct ttctcgttgg cgttctcgcc gccactgatg
     2401 gagcattgcg gccgagcgtt cgccgctgag gttgctccgg ccgattcggc cgccaccgac
     2461 gtcgtggccg cggccctgaa gacgctcttt gccggcggca atccggcagg cgtggcggac
     2521 ctcaaggcca tgcagaccca cgtccagaag ctgaccgagc aactgaagaa gtgcaccgtc
     2581 ggcgtcacgg tcggaatggc gcaaggcagc ggcgtgatcg tcagcaagga cggctacgtg
     2641 ctcacggccg cccacgtggc aggagcgccc aacagccgcg tcgagttcat gctctccgac
     2701 ggcaaggtgg tgcggggcaa gtcgctgggc ctgttccgca cgcgcgacgc cgggctgatg
     2761 aagatcaccg accccggcga ttatcccttc gcggaaatgg gttcgtccac cgaactgaag
     2821 aacgggcagt ggtgcctcgc tttggggcat cccggcggtt accaggagga acgcggcatg
     2881 gtgctgcggg tcggccgcgt gctgctcaac gacctgaccg agaacgtcat cgtcaccgac
     2941 tgcacgctgg tgggcggcga ttccggcggg ccgctgttcg acatggacgg ccacgtcatc
     3001 ggcatcaaca gccgcatcgc cgagtcgttg acctccaaca tgcacgtccc ggtcagcacg
     3061 ttcaaggaaa cgtgggaccg gctgaagaac ggcgaagact ggggccacct gccgggccag
     3121 gagccgtggc tgggcgtgcg cggcgacgac aaggcgaagg acgcgttaat cgcggaagtc
     3181 gtgcctggat cgccggccga tgacgcgggg atcaagaagg gcgacgtgat catctcgtac
     3241 gacggtgaat acattcccga tttcgccgcc ttgaaggcga aggtttcgag ttggcggccg
     3301 acgttcggac ctcgcggctc gcgcccttcc tcgaccgtgc aggtgcttcg cggcggcgag
     3361 gttgtcgaat tgaggctgag acttcgcccg cggggatagg cgcggcccct accgattgcc
     3421 ggaactttct ggagaaactc gtccatgcgc aagtccgctg aagctgcttc gcggcgattg
     3481 ctgccagttg ccgccgtgct actgctcttg ctgcccgcgg cgcgggccca ggatcgcttg
     3541 caccgccggg tgctggaggc ctttcgcccc gtcgtcagcg aaccgtccaa gagcaccacg
     3601 cggatctatt gcgacggctc gcagaaggcg atgggcgcag tcgtccaggc ggacggctac
     3661 atcgtgacca aggccagcga gctctcgggc aacatcgagg tccagacgca cgacatcccc
     3721 aggaagcttt cggcccggat cgtcggcacc gacccgacca ccgatttggc cctgctcaaa
     3781 gtggaggcca aggaattgcc cgttctcgcc tgggcgggtg accccgttcc gccgatcggc
     3841 agctggctgg cgacgcccgg attggagtcg tcgccggtgg ccatcggcat cttgagcgtc
     3901 gctccgcgga agattcccaa tgggggcgcc ttggggatca ctatggaccg ttcgcaggac
     3961 gtgccgcgga tcgtcgaaat caagcctgac tccggggcgg ccaaagccgg cctgcaagtc
     4021 ggcgacatca ttcgccaggt caacaaggac gtcgtcaagg gccagcagaa cctgcgcgag
     4081 acgattggca aatatcaaag gggggacaag gtcacgctgg tcgtcgaacg cgacggcaag
     4141 gagctgaccg ttgaggccac actcggcagc atgagccagc tgatcatggg cgagcgggcc
     4201 ggatttcaaa atggcctggg cggcaagcta agcgaccgcc gcgagggctt tcccttggcc
     4261 ctccagcacg acaccgtgct ccggccttcg gactgcggcg ggccgatcgt cgatctcgac
     4321 ggcaaggccg tgggcctgaa catcgcccgt gccggccgcg tcgagagcta cgccctgccg
     4381 gccgcgctcg cccgcgagtc catcggcaag ctcctcgctc aggccaacac catgccggcc
     4441 gacagcaagc tcgtcgataa gcaaacgccc gcggaagggg agaagaagat ccagtagggt
     4501 gtgacaaggc gggcaccgcc gcgaagcgga ggtcggcggc cgccggcaca ccatggctgg
     4561 catccaatct tggcgggcct gcgctcgccg actcgctggt cccaccctac actggtcgta
     4621 tgcccgatcg cttggaaaag cttcgagcca cgctggcgga actggaggcc gagctgcgcg
     4681 agctggagtc gctcgacgat gcgacccgcg acgtgctggc cgatgccgcc gccgagatcg
     4741 cccgcacgct ccgccgcggc aagcgctcgc ccgaaacgca gcaggccgag tcgtcgcttc
     4801 agggccaaat cgcctcgttc gaagcctccc acccgcagct ggccggcatc gtcactcgcc
     4861 tgatcgacgg cctggggcag ttggggattt gatccgtaga gcaagcgtcc tcgcttgctc
     4921 acgcctccct gcgatcgagc ggtgcacaag cgaggacgct tatgctacgg gccgccgatc
     4981 ttccggaata cgaagtcgac ggccttcaga tcggtctgca gcttgtggtt cttccggacg
     5041 ctcgccagca cggactcgac gtgggcgaat tccgtcgcca gtgcgccatc ggcgatcgtt
     5101 ccctggctgc cgcggccgcg gagtcgcggc tgcgggccgc tgctgttgaa atcgatctgg
     5161 tcctgtccgc cagcgccgtc gaaataggcc gactggaagc cctcggtgcg aatctgcacg
     5221 ccgcccgcgt acaggttccg cgttccgccc cagacccaca acaggtcgcc ggccgcactg
     5281 ccgtaaaacc cggccgcatc gccgctgccg gcagtcgcct ggactgtcac gtcatcgaaa
     5341 ccttcggcgc ggatgctaaa tcccgcgccg cggagccaga cgtagctcgg gttggcgtcg
     5401 agcaagtcag cgcctgcgga atcgcggagc gccgcggcat cgttgccgct gccggcgatc
     5461 accgtcaccg cgccgcagcc gaaggcggcg ctttcgaagc cgccgcccgc tagccgggcg
     5521 aaggctgggc cggcctccag gtagtcgttg gctgccgaat cgtagagcca ggcccgatcg
     5581 ctcgcgccgc cgtaaaaccg cgctgtttcc acgccggtcg cgttcaagct aaagcccccg
     5641 cccacgaact cgacgctgcc cggcctcagg cgcaccgtct cggcgacgct gccgccggtg
     5701 atcgtgagcg tgtcgctgcc gcctttgccc tccaggcgaa tctgcgtcgc gccgccaaga
     5761 gcataggcgg cgccgttgat cgtgatttgc tgtggcagcg cggcattcca cacaaacacg
     5821 tcggccgcca ccgtgccgct gatcgtcgcg atcgacccct gcacgataac cggacccgcc
     5881 gtgccgctgg agccgccccc gccggagttg ccgctgctgg aggtcggcgc ggccaggacg
     5941 tcgatggtgt acttgccgat cgagccgccg
//
LOCUS       DASRJB010000913         6423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1964406, whole genome shotgun sequence.
ACCESSION   DASRJB010000913 DASRJB010000000
VERSION     DASRJB010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964406"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..2110
                     /gene="typA"
                     /locus_tag="VFB80_24025"
     CDS             275..2110
                     /gene="typA"
                     /locus_tag="VFB80_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZN36928.1"
                     /translation="MRRQDLRNIVIIAHVDHGKTSLVDALLRQSGQFRESQLQGDCIL
                     DSNDLERERGITILAKNIALPYKGVKINIIDTPGHADFGGEVERVVRMADGALVLIDA
                     AEGPMPQTRFVLSKALEAGLRPIVIINKIDRPDGQPHATLDEAFGLLMELDAEEHLED
                     FKYLFASGREGYATADPAVRTESMQPLLDMVLESIPGPKVEEGAPLQMLVTTLDWSEF
                     VGRIAIGRIQAGSIKTGQTIALMKSEGRVTESRVTQLFTFDKLGRVLVDEVTAGDVCA
                     VVGIGDVEIGDTICHREHHRPLERLHVDEPTLEMVFTINSSPFAGREGKYVTNRQLRE
                     RLLKELEKNVALRVRQIEHSDAFAVSGRGVLHLSVLIETMRREGYELSVGKPKVILKT
                     IDGEKHEPFETLVVEVPSEKLGSVMELVGARRGELEEMHVRGQYTHARFMIPARGLIG
                     LRTRLLNSTQGTAIIHHRYAGYKPMQGEVPRRPNGVYISMVGGRANAYSLNTLQERCD
                     MFVTHNDEVYQGMIVGENSRDNDLAVNPTKEKKLTNMRASGSDENILLKPPRKMDLEA
                     ALEYIEDDELVEVTPTQIRLRKILLTENDRRRQTRKQQDQLTAAG"
     gene            complement(2159..2746)
                     /locus_tag="VFB80_24030"
     CDS             complement(2159..2746)
                     /locus_tag="VFB80_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN36929.1"
                     /translation="MSHYRRNYVEGGKYFFTVVTYRRRRMLTTDLARECLRAAIETIK
                     RDRPFEIVALVLLPDHLHSVWTLPRGDADYSTRWRRIKEEFTKQYLASGGAELPQSRS
                     RREHGQRGIWHKRFWEHTVRNEAELERSCDYVHWNPRKHELVARVADWPYSTFHRFVR
                     AGQYDLEWGGTDPTPGYDTPEWVLLEPDADLPEEQ"
     gene            complement(2948..3586)
                     /locus_tag="VFB80_24035"
     CDS             complement(2948..3586)
                     /locus_tag="VFB80_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36930.1"
                     /translation="MSRFRFSIATFLTVVTLLALGLAAMASQSRLATSLAYTAFLVLL
                     AVASAGALLAAPPKRAFWIGFAIFGWTYWFVEFDVTQARSSGPQPIVTTISMFSGQLT
                     TRPQSSAPSSLLTAELMDWLESVMTANLSVGSKVTAQWRGGSYYTGTIVQAQNGQYLI
                     VWDDGSPQQWTPPSQIMPNSPNLRLAGHATLGGLFALLGGVIVAVLFGERKV"
     gene            complement(3645..4664)
                     /locus_tag="VFB80_24040"
     CDS             complement(3645..4664)
                     /locus_tag="VFB80_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HZN36931.1"
                     /translation="MQALQIVAPKELQLIEQAEPAQPRAGEALARVHCVGICGTDVSA
                     YLGKMPFVTFPRILGHELGVEVLAVGVGVTNVKAGHRCSVEPYINDPDSYASRRGRPN
                     CCERLQVLGVHTDGGMRPRIIVPARKLHPASKLTFEQLALVETLAIGCHAVARSGLAA
                     GETCLIIGAGPIGLATLEFVKLAGARPIVVDLSQSRLDFCRRMMGVEHVLPVDGQLER
                     RLRDVAGGQLPEVVIDATGNPQSMSAAFGLVAPGGRLVYVGLTGEEIRFRHSAFHKVE
                     GTLLCSRNALPGDFTRIIGLIEAGRLDTQPWISHRCDLADLPAQMPAWLKPETGVVKA
                     MVAVQ"
     gene            complement(5223..>6423)
                     /locus_tag="VFB80_24045"
     CDS             complement(5223..>6423)
                     /locus_tag="VFB80_24045"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36932.1"
                     /translation="QTASASGGRQPADAVPLKELLARADLKDYASWLAAAARGDRAVK
                     ARLSEVDRWADATLDKWYGQNRKREPHFAEALHAARQMLPDAESKLLELLGNRTQPAV
                     ARATAALEIGAYLEPAGAVANALRKAAAESDPQLKAAAILSLQPDGSDATISVLAAML
                     SPKETRLVRTESARALVQMTPDRLTGEERAAFREAIEECIRGVMEASDRSASHLNIAA
                     MYEGLGNLQGAEEAYQTALKVEPRAVGARANLAALYEQQIEMAQRRGIERARSGDRAG
                     AEQEMAAVAHLLEVVPRLRAEELELLERDLMLAPHLSAVLERTGLIRYTVGWRKEAES
                     ALLTASLMEPRNPLYLFHLAVLYRDTGRPEQAVPLAGRLLKLRPNNPSFQHFARELAQ
                     PIGPAPP"
BASE COUNT         1163 a         2139 c         2070 g         1051 t
ORIGIN      
        1 ggtcgatcag gtcgcctacg tccgcttcgc cagcgtctac cgccgcttca aggacgtccg
       61 cgacttcgtc gatgagctgc agccgatgct gaagcgcaac ctgccgccgc ggtagggtgg
      121 gaccagcgag cggcagctcg cgccggccca ccattccgac gcggtgggcc ggcggctgcg
      181 ccttgtccca ccctataaac acccctaggc cacttcggcc agcggctgct acgatcaact
      241 ttgaaccttc aactcggaac tttgaacttg ccccatgcgc cgccaggacc ttcgcaacat
      301 cgtcattatc gcccacgtcg atcacgggaa gacgtcgttg gtcgatgcgc tcctgcggca
      361 gagcggccag tttcgcgaaa gccagttgca gggcgactgc atcctggata gcaacgacct
      421 ggagcgggag cgcgggatca cgatcctggc caagaacatc gccctgccct acaagggcgt
      481 gaagatcaac atcatcgaca cgccgggcca cgccgacttc ggcggcgagg tcgagcgggt
      541 cgtgcggatg gccgacgggg cgctggtgct gatcgacgcc gccgaggggc cgatgcccca
      601 gacgcggttc gtgctcagca aggccctgga ggcaggcctg cggccgatcg tgattatcaa
      661 caaaatcgac cggccggatg gtcagccgca cgcgacgctg gacgaggcgt tcggcctgct
      721 catggagctc gacgccgagg agcacctgga ggacttcaag tacctgttcg ccagcggccg
      781 cgagggctat gccacggcgg accccgccgt gcggaccgaa tcgatgcagc cgcttttgga
      841 catggtgctg gagtcgattc ccggcccgaa agtcgaagag ggcgcgccgc tgcagatgct
      901 ggtgacgacg ctggactggt cggagttcgt cggccggatc gcgatcggcc gcatccaggc
      961 cggttcgatc aagaccggcc agacgatcgc cctgatgaag tccgagggcc gcgtcaccga
     1021 gtcgcgggtc acgcagctgt ttacgttcga caagctcggc cgcgtgctgg tcgatgaagt
     1081 aactgcgggc gacgtctgcg ccgtcgtcgg catcggcgac gtcgaaatcg gcgacacgat
     1141 ctgccaccgc gagcaccacc ggccgctgga gcggctgcac gtcgatgaac cgacgctgga
     1201 gatggtcttc acgatcaaca gctcgccgtt cgccggccgc gaaggcaaat acgtcaccaa
     1261 tcgccaattg cgcgagcggc tgctcaagga actggagaaa aacgtcgccc ttcgcgtgcg
     1321 gcagatcgag cacagcgacg cctttgccgt ctccggccgc ggcgtcttgc acctctcggt
     1381 gctgatcgaa accatgcgcc gcgaaggcta cgaactgtcg gtcggcaagc ccaaggtcat
     1441 tctcaagacg atcgacggcg agaagcacga gccgttcgag acgctggtgg tcgaggtccc
     1501 ctccgagaag ctcggcagcg tgatggagct ggtcggggcc cgccgcggcg agttggaaga
     1561 gatgcacgtc cgcgggcagt acacccacgc ccgcttcatg atccccgccc gcggcttgat
     1621 cggcctgcga acgcggctgc tcaactccac gcaaggcacg gctatcattc accaccgcta
     1681 cgcgggctac aagccgatgc agggcgaagt gccgcggcgg cccaacggcg tctatatctc
     1741 gatggtcggc ggccgggcca acgcctacag cctgaatacg ctgcaggagc ggtgcgacat
     1801 gttcgtcacg cacaacgacg aggtctatca agggatgatc gtcggcgaga acagccgcga
     1861 caacgacctg gcggtgaatc ccacgaagga aaagaagctg acgaacatgc gggccagcgg
     1921 cagcgacgag aacattctgc tcaagccgcc gcggaagatg gacctggagg ccgctctcga
     1981 gtacatcgag gacgacgagc tggtcgaggt tacgccgacg caaatccgcc tccgcaagat
     2041 cctgctcacc gaaaacgacc gccgccgcca gacccgcaag cagcaggatc agcttacggc
     2101 tgcggggtag ggtagggtgg gtcaaggccg ccgcggccgc agacccacgc ggcgcagact
     2161 attgctcctc aggcaagtcc gcgtccggct ccagcagtac ccattccggc gtgtcataac
     2221 cgggtgtcgg atcggttcct ccccattcca gatcgtattg gccagcgcga acgaatcgat
     2281 gaaacgtcga atatggccag tcggccacac gggccacgag ctcgtgcttt cgcgggttcc
     2341 agtgcacata gtcgcagctt cgctccagct cggcttcatt gcgcacggtg tgctcccaga
     2401 agcgtttgtg ccaaatgccg cgctgaccgt gctcgcgccg cgagcgcgat tgaggcagct
     2461 cggcgccgcc gctggcaagg tactgcttcg tgaattcctc cttgatccgt cgccaccgcg
     2521 tggaatagtc ggcgtcgcct cgcggcaacg tccagacaga gtgcaagtgg tccggcagta
     2581 agaccagcgc cacgatttca aacggccggt cgcgcttgat cgtctcgatt gccgcgcgca
     2641 ggcactcgcg cgccaagtcc gtcgtcaaca ttcggcggcg gcgatacgtg accacggtga
     2701 aaaaatactt gccaccctcg acgtagttac ggcggtagtg ggacatggca ggttccgaat
     2761 tgcccacagc atagtcgctg ccgagcaaga atccgtaggg tgggtcaagc tcgcgcgagc
     2821 aattttgcca tcgcgaatgt cacggtcgcg agcgctgacc cacgctcggt caccgcacag
     2881 tttaccgctc cccatcgcca acgtcggcgc cgcgtgggtc tgcgctcgcg gactcgcttg
     2941 acccacccta cactttgcgc tccccaaata agaccgcgac gatcacgccg ccgagcaggg
     3001 cgaagaggcc gcccaaggtg gcgtggccgg cgaggcgcag attcggcgaa ttgggcatga
     3061 tctggctggg gggcgtccac tgctgcggac tgccgtcgtc ccagacgatc aggtactgcc
     3121 cgttctgggc ctgcacgatc gtgccggtgt agtacgagcc gccgcgccac tgggcggtga
     3181 ctttggagcc gaccgacagg ttggccgtca tcaccgattc cagccagtcc atcagctcgg
     3241 ccgtcagcag gctcgacggc gccgacgact gcggccgcgt cgtgagttgc cccgagaaca
     3301 tggaaatcgt ggtcacgatc ggctgcggcc cgctgctgcg agcctgagtc acgtcgaatt
     3361 caacgaacca atacgtccag ccaaagatgg caaagccgat ccagaacgcc cgcttcggcg
     3421 gcgctgccag caacgcccct gccgacgcca cggcgagcag caccaggaac gcggtatagg
     3481 ccaggctcgt ggccagccgc gactgcgacg ccatcgccgc cagccccagg gccagcagcg
     3541 tgaccacagt cagaaatgtg gcgatcgaga agcgaaagcg ggacataagc ggctatggac
     3601 caaatcacaa gcagcaacca tccaaccgaa tttcagacat tctgtcattg taccgcaacc
     3661 atcgccttaa cgacgcccgt ctcgggcttg agccaggccg gcatctgggc cggcaggtcg
     3721 gccaggtcgc agcggtgcga aatccacggc tgcgtgtcga gacgcccggc ttcgatcagc
     3781 ccgatgatcc gcgtgaagtc gccgggcagg gcgtttcgcg agcaaagcag cgtcccttca
     3841 accttgtgaa acgccgagtg ccggaagcga atctcttcgc cggtcaggcc gacataaacc
     3901 agccggccgc cgggcgcgac gagaccgaac gcggccgaca tcgactgcgg attgccggtc
     3961 gcgtcgatca ccacttccgg caattggccg ccggcgacgt ctcgcaagcg gcgctcgagc
     4021 tggccatcga ctggcaggac gtgctcgacg cccatcatgc ggcggcagaa atccagccgc
     4081 gattggctca gatcgacgac gatcggccgc gcccccgcca gcttgacgaa ctccagcgtc
     4141 gccaggccga tcggcccagc gccgatgatc aggcacgttt cgcccgccgc caggccgctg
     4201 cgggccaccg cgtggcagcc gatggccagt gtttcgacca gggcgagctg ctcgaaggtc
     4261 agcttcgatg cgggatgcag cttgcgggcc ggcacgataa tccgcggccg catgccgccg
     4321 tcggtgtgta cgcccaggac ttgcagccgc tcacagcaat tcggccggcc gcggcggctg
     4381 gcatagctgt ccgggtcgtt gatgtacggc tcgaccgagc agcggtggcc cgccttgacg
     4441 ttggtaacgc ccacaccgac cgccagcacc tccacgccca gctcatgccc caggatccgc
     4501 ggaaaggtca cgaacggcat cttgcccagg taggcgctga catccgtgcc gcaaatcccc
     4561 acgcaatgca cccgggccag cgcctcgccg gcccgcggct gggccggctc cgcctgctcg
     4621 atgagctgaa gctctttcgg ggctacgatc tgcaaggcct gcatggtcgg attcgttttt
     4681 cgccaggtcg gcgggctccg tggcgaatta atcggccgta ctcgaattga cgcagtggcc
     4741 ctaagttgtg tcgccggcta gacttgcggc ctcaggctcg ttccaaagca cgccgaataa
     4801 ttcgccaaac gggcccgata cctggggagc tggcgaataa ttcgcgccgc tcggcgtaac
     4861 tgccgacggc gtctggactt gaatttttcg ccatggcgaa aaattcagcg ctgagcgaat
     4921 tgagaaagag cgtccatacc acaacgctcg gcacgggtga gtacggatta caggccgcca
     4981 ccaaggcacc gctctgcggc tctgttcgcc gagcaaacga cgtggtatcc agaccgcatt
     5041 atccgcgcaa tcctgaggtt ggcaagctac ttgcacacat tttgttcatg atgtgccaac
     5101 ttgcgccgaa aggcgtcgac gtaagtccag tcccaccggg cacttccgct ccggcgaatt
     5161 gacgtaagtt tttttcgcac ccgctagcgg aatggactag tgtggtccat tctggatgct
     5221 ccctacggtg gcgcgggacc gatcggctgg gccagctcac gggcgaaatg ctggaagctg
     5281 gggttgttcg gacgcagctt gagcagccgg ccagcgagtg gaaccgcctg ctccggccgg
     5341 ccggtgtccc gatagagcac cgcgaggtga aagaggtaga gcggattgcg gggctccata
     5401 agcgacgcag tgagcagcgc cgactcggcc tccttgcgcc agccgacggt gtagcgaatg
     5461 aggccggtcc gctccaggac ggcggacagg tgcggagcca gcatcaagtc ccgctccagc
     5521 aactcgagtt cttcggcccg caggcgaggc acgacttcca gcaggtgggc cacggcggcc
     5581 atttcctgct ccgcgccggc ccggtcgccg ctgcgggccc gctcgatgcc ccggcgctgg
     5641 gccatctcga tctgctgttc gtacaaagcg gcgaggttgg cccgcgcgcc gaccgcccgc
     5701 ggctcgacct tgagggccgt ctgatacgct tcctccgcgc cttgaaggtt gccgaggcct
     5761 tcgtacatcg cggcgatatt gaggtggctg gcgctgcggt cgctggcctc catcacgccg
     5821 cggatgcact cctcgatcgc ttcgcgaaat gccgcccgct cctcaccggt caagcggtcg
     5881 ggcgtcattt gcaccagcgc ccgggccgac tcggtgcgca ccagccgcgt ttccttgggc
     5941 gacagcatgg ccgccagcac ggagatcgtg gcatcgctgc cgtcgggctg caggctcaag
     6001 atggccgcgg ccttgagctg gggatcgctt tcggctgccg ccttccgcag cgcattggcg
     6061 accgcgcctg cgggctccag atacgcgccg atctccaggg ctgcggtcgc acgggccacc
     6121 gccggctgcg tgcgattgcc cagcagctcc agcagtttcg attccgcatc cggcagcatc
     6181 tgccgggcgg cgtgcagggc ctcggcaaag tgcggctcgc gcttgcgatt ttgcccgtac
     6241 cacttgtcga gcgtcgcgtc ggcccagcga tcgacttcgg acaggcgggc cttcaccgcc
     6301 cggtcgccgc gggcagcagc ggcgagccag ctcgcgtagt ctttgaggtc ggcgcgggcg
     6361 aggagttcct tgagcggcac ggcatcggcg ggctgacgcc ccccgctcgc cgaggcagtc
     6421 tgt
//
LOCUS       DASRJB010000914         8743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3652157, whole genome shotgun sequence.
ACCESSION   DASRJB010000914 DASRJB010000000
VERSION     DASRJB010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3652157"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFB80_24050"
     CDS             complement(<1..335)
                     /locus_tag="VFB80_24050"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN36933.1"
                     /translation="MRRLLSYAALLLLAALSRAANGEEAVEFARDVQPTLQRHCTKCH
                     GGVRREAGLSLLAPGGVGESGKPLVVAGKPDESELLRRVSSADADERMPAEGPPLSAA
                     EIAALRRWI"
     gene            complement(338..3196)
                     /locus_tag="VFB80_24055"
     CDS             complement(338..3196)
                     /locus_tag="VFB80_24055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN36934.1"
                     /translation="MFSAIVAAWPALLSAAEPARIPWTTSRVTGSPDPPPPFKVVRAF
                     PNLKFDKALLLARCPGSDRLFVGEQAGVLYSFPNRPDAKAELFCDLRKELTTIARHPD
                     AKGIEAVYGLVFHPDFEKNRQCFVCYTLEPKSGIRNLPEGSRVSRFTVTASDPPRIDP
                     ASEEIVITFLQGGHNGGDLHFGKDGMLYISTGDAANPNPPDRHDTGQDISDLLSSVLR
                     IDVNRKDAGLNYAVPPDNPFVGMPKARPEVWAYGFRNPWRMSFDRLTGELFVGDVGWE
                     LWELVHRVEKGGNYGWAAMEGPQPIKSEPVGPTPIHPAAIELSHTIACSITGGYVYRG
                     QKFPELVGAYVFGDWETRRLWAARFEGDRLKEMPEITRPTVRFAAFGEDNAGELYFLD
                     YDSGTLHTLERNDGGSQNADFPRALSQTGLFASVKDHQPAAGVIPFSPNSRQWLDGAT
                     SEHFFAFPGESSATLYESGRPVPGLVYWHNFRLHFPKDAVLVRTISLGGKRLETQLMH
                     FAGEDWRGYTYVWRDDQTDADLAPADGAEKEVQLGGQKRTWQFLSRSQCLSCHSNQSE
                     YALSFLPEQLNRLGTDGRNQLVSLTEAGYIRRVGNNGQAQPPFDGATAARQKSVTDPT
                     DESQPLDARARGYLHANCGHCHSDHGGGAVALRLQFQTPAGELNAVDAKPTRGDFGLP
                     DAAIIKPGDPYASTLFFRMAKFGRDRMPHIGSELPDEAGLALIEPWIAGMKEGPTVFS
                     PDGADLSNPRTALMAARKIGRGDLPPAEREALLAHAAKLAPGPVRDLFDGYLPADEKG
                     KRLGSSPRPATILALHGDAKRGEALFWSMAVNCGSCHKIGERGTSLGPDLSAIGKLRA
                     RDDLLQSLLEPSRRIEPKFAAYLARASGGSVVTGLVMKRDAGGVVLRDAQNKEVALAA
                     EEIDQMQPSRRSLMPDGQMAGLTPQEAADLLEYLATRQ"
     gene            complement(3207..3803)
                     /locus_tag="VFB80_24060"
     CDS             complement(3207..3803)
                     /locus_tag="VFB80_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36935.1"
                     /translation="MIRSWLMSIAMLIAAQSAARAEQFVLFDVTFTFTKEDADNSKPS
                     KSHFYVKGDMLNPNRPKDWTTPVDYQSGTVHIRAEVLEKPAGGEPTTWTLCYIPNKGQ
                     KNGYGCTGTDLYREKGVYEKDVSMKSFWQNDSIVWSEGIKQMDLVIKDGSGGSGHAHK
                     RPDHEKFFPTKMRITMIQVSAGAKYDPSLVPNLPASKP"
     gene            3937..7071
                     /locus_tag="VFB80_24065"
     CDS             3937..7071
                     /locus_tag="VFB80_24065"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36936.1"
                     /translation="MLPKPILGPFVAALLVATCCLAQEPRRDIRLSIEPPHISSDKSV
                     RYDFDIVYVRGLRRTDGKEADWAEFSRPTTMEPGADLMLLHPDGSEEVLVSGADGSVM
                     DPYVSFDGQSVYYAKFIDSRHTGSDIFRVHVPSRKIVRLTDQTFTPNTGAAPWSSDYR
                     TPEEGKTALSYGVYNLGPCPIPGGKVLYTSNRNAHVPPRAYPRQTLQLHLMDDDGRNI
                     EQVGYLNIACALHPVILRDGRVLFSTLESHALHNAIMWGIWSIHPDGTNWNPVISAFE
                     NDGAPSAYHFQTQLSDGSIVIEKYYNQNQKGFGTLLKLPETPPAGQPAFGPGNREDPR
                     NRVSFLGADGRQQAHAVPFSPAGMELITPWIHWEDRPSPPAILADENSPRIGKVTHPC
                     GAPDNHLLVAWTLGPIGGSSGSVREFMVPKQIDSGLCLIRGGKPTGQPGDMLLIKNDP
                     KYNEQWPRPLVSYERIYGVKEPKRLIHKNDGHKNPALPEGTPFGLVGTSSLYKRESAP
                     GGVVPEGSVTAVARPKNPSEWYWSPWRTNWGLQGSDAGLYDNSEIHAIRIVAQEPRTD
                     IQGRGRDGAPRYGSHGLERLRILGEIPVRKFSGGKPEPLDPDGNPDTSFLAKIPADQS
                     FTFQLIDKEGMTLTMAQTWHQLRPGEVRYDCGGCHAHSQKPTPFEQTAAALSDYRLFD
                     LTTGKTPLVTDKGRDESQRQWDAADKTGLRYADGGVANVEYFRDIRPILAKSCVACHT
                     HQAAEPPGGLVLDDDDLGKQTAFGADAVSEVQVPNTYFRLAAWKRYAPSPPGAMATPQ
                     AASPYVTMYQSRRSWLVWKLYGRRLDGWSDDDFPSLKKVGDLKSLVGPGGRPIEKLDY
                     SDEKALAQFVRSRSIDRDFSGSTMPPPEAVKAGKVQPLSDEDRRTIVRWIDLGCPIDL
                     DPRYDPAQAGSRSYGWMCDDQRPTLAVTLPREGGNKLLSHILIGMADAYTGLDPGSFT
                     VVADFPVDGAAAGENLASKFTALPGNRWQLKLARPIDDLPQGTLTVSVKDKQGNIQRV
                     KREFSAGPSLASSQ"
     gene            complement(7060..8616)
                     /locus_tag="VFB80_24070"
     CDS             complement(7060..8616)
                     /locus_tag="VFB80_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN36937.1"
                     /translation="MPGRLARLLADYVPLWHRLMRLPGQLFAISPFDGDSRPSRVRLF
                     QPGSGRPTLPFPHPWNYDQPIRILESYFWGAESETGPGRHNRYLDIPGCAPILVTRDP
                     AMIRAIAAETGDRDGQFDRDTLPSTGIARATGSDTLLFANGPLWRRQRKLAASPFGKT
                     TLFQPEMFHEFAATFRGTIVRRLGVLREHLQSSGKSSVRVPLEPEIKAVMLEMLANNF
                     FSAELSYEQVRNRYVPALERVIDHIVSDTVTNKLGLPISWLPSFTRRIAQTKADYACF
                     DELTTLVLAPRKEGKGLWRQFKSDAPDAALRSNLKVFLAGALEATTSFASWALAHLAR
                     NQAAQEKVYQEVKDVDDYNPDNLDAARYLGQVLDETLRLTPSLYFLPRRATADTWVET
                     ADGRKMFIPRGTHILLDVWHANRHEDHWGAAVTGYPASEFVPERWTNLAEQGRGSKEI
                     LHFGFGHGPRVCPGKHLGQLEVGLVVGAVVKMFRFQAVHQGNPAKAGVSTKPQDGTLV
                     ELQLRQNSLR"
BASE COUNT         1464 a         2922 c         2898 g         1459 t
ORIGIN      
        1 tcgatccagc ggcgaagcgc ggcgatctcc gcggccgaca gcggcggacc ctcggctggc
       61 atccgctcgt cggcgtcggc actggagacg cggcgcagca gctcactctc gtccggcttg
      121 cccgcgacga ccagcggctt gcctgattcg ccgacgccgc ccggcgcgag caggctcaat
      181 cccgcctccc gccgcacgcc gccgtggcac ttcgtgcagt gccgctgcaa ggtcggctgc
      241 acgtcccgcg cgaactcgac cgcttcctct ccgttcgccg cccgggaaag ggcggccagc
      301 aacaggagcg cggcatagct caggaggcgg cgcatggcta ctggcgggtg gcgaggtact
      361 ccagcaaatc ggctgcttcc tgcggcgtca ggcccgccat ctggccgtcg ggcatgagcg
      421 agcggcggga cggctgcatc tgatcgatct cctccgccgc caaggcgact tccttgttct
      481 gggcgtcgcg gagcacgacg ccgcctgcgt cgcgcttcat gaccaggccg gtgacgacgc
      541 tgcctcctga cgcgcgggcc agataagcgg caaatttggg ctcgatccgc cgcgacggct
      601 ccagcaggct ctgcagcaaa tcgtcccgcg cgcggagctt gccgatcgcc gacaggtccg
      661 gccccagcga ggttccgcgc tcgccgatct tgtggcaact gccgcagttg accgccatgg
      721 accagaacag ggcttcgcca cgcttcgcgt cgccgtgcaa cgcgagaatc gtcgccggcc
      781 gcgggctgga gccgagccgc ttaccttttt cgtcggcggg cagatagccg tcgaacaggt
      841 cgcgaaccgg cccgggcgca agcttcgccg cgtgcgcgag cagcgcttcc cgctcggcgg
      901 gcggcaagtc gccgcgtcct atcttgcggg ccgccatgag ggccgtccgc ggattggaca
      961 aatcagcacc atcgggcgag aacacggtgg gaccttcctt catgccggcg atccatggtt
     1021 cgatgagcgc gagccccgct tcgtctggca actccgagcc gatgtgcggc atccggtcgc
     1081 ggccgaactt ggccatgcgg aagaaaagcg tgctggcgta gggatcgccc ggcttgatga
     1141 tcgctgcgtc tggcagtccg aagtcgccgc gcgtgggctt cgcgtccacc gcgttcagct
     1201 cgcccgccgg cgtctggaac tggagccgca gggcgaccgc cccgccgccg tggtcggagt
     1261 ggcagtggcc gcagttggca tggaggtagc cgcgggcccg cgcgtccagc ggttggctct
     1321 cgtcggttgg atcagtgacg ctcttctgac gagcggccgt ggcgccatcg aacgggggct
     1381 gagcctggcc attgttgccg acacggcgga tgtatcccgc ctcggtcagc gacacgagct
     1441 ggttgcggcc gtcagtgccg agtcggttca gctgctcggg caggaacgac agtgcatact
     1501 ccgactggtt gctgtggcac gacaggcact ggctgcggct gaggaattgc caggtgcgct
     1561 tctggccgcc gagctgcact tccttctccg cgccgtcggc gggagccagg tcggcgtcgg
     1621 tctggtcgtc gcgccagacg taggtgtagc cccgccagtc ctcgccggca aagtgcatca
     1681 gctgcgtctc cagccgcttg ccgccgaggg aaatcgtccg caccagcacc gcgtctttgg
     1741 gaaagtgcag gcggaagttg tgccagtaaa cgagccccgg aaccggcctt cccgactcgt
     1801 agagcgtagc cgacgactcg cccgggaacg cgaagaagtg ctcgctagtc gccccatcca
     1861 gccactgccg gctgttcggc gaaaacggta tcacgccggc cgccggctga tggtccttga
     1921 ccgatgcgaa caggcccgtc tgcgagagcg cccttggaaa atccgcgttc tgcgagccgc
     1981 cgtcgttgcg ctcgagcgtg tgcagcgtgc cgctgtcgta gtcgaggaaa taaagctcgc
     2041 ccgcgttgtc ttcgccgaag gcggcgaagc ggacggtggg ccgcgtgatc tcgggcatct
     2101 ctttcaggcg gtcgccctcg aagcgggcgg cccacaggcg gcgggtctcc cagtcgccga
     2161 agacgtaggc cccaacgagc tccggaaatt tctggccgcg atagacgtag ccgccggtga
     2221 tgctgcaggc gatcgtgtgc gacagctcga tcgcggccgg gtgaatcggc gtcggcccga
     2281 ccggctccga cttgatcggc tgcggacctt ccatcgcggc ccagccgtag ttgccgcctt
     2341 tttccacgcg gtggaccagc tcccacaatt cccagccgac gtcgccgacg aacaattcgc
     2401 cggtcaggcg gtcgaagctc atccgccagg gattgcgaaa gccgtaggcc cagacttcgg
     2461 gccgcgcctt cggcattccg acgaaaggat tgtcgggcgg cacggcatag ttcaatcccg
     2521 cgtccttgcg attcacgtcg atgcggagga ccgacgagag caggtccgag atgtcttgcc
     2581 ccgtgtcgtg gcggtcgggc ggattggggt tcgccgcgtc gcccgtcgag atgtagagca
     2641 tcccgtcctt gccgaagtgc aggtcgccgc cgttgtggcc gccctggagg aaagtgatga
     2701 cgatctcctc gctggccggg tcgatccgcg gcggatcgct ggccgtgacc gtgaatcgcg
     2761 acacccgcga gccctccggc aggttccgga tgccgctctt gggctccagc gtgtagcaga
     2821 cgaaacactg gcggttcttc tcgaaatccg gatggaacac cagcccgtat acggcctcga
     2881 tgcccttcgc gtcgggatgc cgggcgattg tcgtcagctc cttccgcagg tcgcagaaca
     2941 gctcggcctt ggcgtccggc cgattcggga acgaatagag cacgcccgcc tgctcgccga
     3001 cgaacagccg gtcgctgccg gggcagcggg ccagcagcaa ggccttgtcg aacttcaaat
     3061 tcgggaaggc ccgcacgacc ttgaagggcg gaggcggatc aggcgagccg gtgacccgcg
     3121 aggtggtcca gggaatgcgc gcgggctcgg cggccgatag cagcgccggc caggcggcga
     3181 cgattgcact gaacaagacg agccagctag ggcttggacg ccggcaggtt gggaacgagg
     3241 ctgggatcat atttcgcgcc tgcggagacc tgaatcatcg taatccgcat tttcgtcggg
     3301 aagaactttt cgtgatcggg ccgcttgtgg gcgtggccgc tgccgccgct gccgtccttg
     3361 atgaccaggt ccatctgctt gatgccctcg gaccagacga tcgagtcgtt ctgccagaac
     3421 gacttcatcg agacgtcctt ttcgtacacg cccttctcgc ggtacaggtc ggtgcccgtg
     3481 cagccatagc cgttcttttg gcccttgttg gggatgtagc agagggtcca ggtggtgggc
     3541 tcgccgccgg cgggcttctc gaggacctcg gcccggatgt ggacggtgcc gctctggtaa
     3601 tcgaccggcg tcgtccaatc cttggggcga ttggggttaa gcatgtcgcc tttgacatag
     3661 aagtgcgact tgctcggctt cgaattgtcg gcgtcctcct tggtaaaggt gaacgtcacg
     3721 tcgaagagga cgaactgttc ggcgcgagcc gcggactgcg ccgcaatcaa catcgcgatg
     3781 ctcatcagcc agctgcggat catggcgctg cctccgggtg ttgccgctca gtatatccca
     3841 gcggcgggcg gcatttgctt tgacaccgcc gcgccggcgg ctttagattg ttgacgcccg
     3901 ccttgtgctc ccaacttacc tgccgggagt ggaacgatgt tgcccaaacc gatcctcggc
     3961 cctttcgtcg ctgcgttgct ggtagcgact tgttgtttgg cccaggagcc gcggcgcgac
     4021 atccgcctga gcatcgagcc gccgcacatt tcgagcgaca agtcggtccg ctacgacttc
     4081 gacatcgtct atgtccgcgg cctgcggcgg actgacggca aggaggccga ttgggccgag
     4141 ttctcgcggc cgaccacgat ggagcccggg gccgacctga tgctgctgca tccggacggc
     4201 agcgaagagg tgctggtcag cggcgcggac ggctcggtaa tggaccccta tgtctcgttc
     4261 gacggccagt cggtctatta cgccaaattc atcgactcgc ggcacaccgg ctcggacatc
     4321 tttcgggttc acgtgccatc gcgaaagatc gtccgcctga ccgaccagac cttcacgccg
     4381 aacaccgggg ccgccccgtg gtcgagcgac taccgcacgc ccgaggaagg caagaccgcc
     4441 ctctcgtacg gcgtctataa cctgggaccg tgcccgatcc ccggcggcaa ggtgctgtac
     4501 acgagcaacc gcaatgctca cgtgccgccg cgggcctatc cgcggcagac gctgcagctg
     4561 cacctgatgg acgacgacgg ccgcaacatc gagcaggtcg gctacctcaa catcgcctgc
     4621 gccctgcacc cggtaatcct ccgcgacggc cgcgtcctgt tcagcacgct tgaatcgcac
     4681 gccctgcaca acgcgatcat gtggggcatc tggagcatcc accccgacgg cacgaactgg
     4741 aacccggtca tcagcgcctt cgaaaacgac ggcgcgccct cggcctacca ctttcagacg
     4801 caactttccg acggctcgat cgtcatcgag aaatactaca accagaacca gaaaggcttt
     4861 ggcacgctgc tcaagctgcc cgagactccg cccgcgggcc agcccgcgtt cggcccggga
     4921 aatcgcgagg acccgcgcaa ccgcgtctcg ttcctggggg ccgacggccg ccagcaggcc
     4981 cacgccgtcc ccttctcgcc cgcggggatg gagctgatca cgccctggat tcactgggag
     5041 gatcggccgt cgccgccggc gatcctcgcc gacgaaaact cgccgcggat cggcaaggtg
     5101 acgcatccct gcggcgcgcc cgacaaccat ctgctcgtgg cctggacgct cggccccatc
     5161 ggcggctcca gcggctcggt ccgcgagttc atggtcccca agcaaatcga ctccgggctc
     5221 tgcctcatcc gcggcggcaa gccgaccggc cagcccggcg acatgctgct catcaaaaac
     5281 gatccgaaat acaacgagca gtggccgcgg ccgcttgttt cgtacgaacg catttacggc
     5341 gtgaaagagc cgaagcggct gatccacaag aacgacggcc acaagaaccc ggcgcttccg
     5401 gagggcacgc cgttcgggct cgtgggaacg tccagcctct acaaacgcga gagcgccccc
     5461 ggcggcgtcg tgcccgaagg cagcgtcacc gcggtggccc gcccgaagaa tccgagcgag
     5521 tggtactggt cgccgtggcg gaccaactgg ggcctgcaag gctccgacgc cggcctctac
     5581 gacaacagcg aaatccacgc catccgcatt gtcgcccagg agccgcggac cgatatccag
     5641 ggccgcggcc gcgacggcgc gccgcgctat ggcagccacg gcctggagcg gctgcgaatc
     5701 ctgggcgaga ttcccgtgcg gaagttctcc ggcggcaagc cggagccgct cgatcccgac
     5761 ggcaatccgg acacgagctt cctggccaaa attcccgccg accagtcgtt cacgttccag
     5821 ctgatcgaca aggaaggcat gacgctgacc atggcccaga cgtggcacca actgcggccg
     5881 ggcgaggtcc gctacgactg cggcggctgc cacgcccaca gccagaagcc gacgccgttc
     5941 gagcagacgg ccgccgcgct gagcgactat cggctgttcg acttgaccac gggcaagacg
     6001 ccgctggtga ccgacaaagg ccgcgacgag tcgcagcggc agtgggacgc cgcagataaa
     6061 acgggcttgc ggtacgccga tggcggcgtg gccaacgtcg agtattttcg cgacatccgg
     6121 ccgatcctgg ccaagagctg cgtcgcctgc cacacgcacc aagcggccga gccgccgggc
     6181 ggcctggtgc tcgacgacga cgacctcggc aagcagaccg ccttcggagc cgacgcagtg
     6241 tcggaggtgc aagtgccaaa cacctatttc cggcttgcgg cgtggaagcg ctacgccccc
     6301 tcgccgcccg gcgcgatggc tacaccgcag gcggccagcc cgtacgtcac catgtatcag
     6361 tcccgccgca gctggctggt ctggaagctc tatggccggc ggctcgacgg ctggtcggac
     6421 gacgattttc cgtcgctcaa gaaggtcggc gatctgaagt cgctggtcgg ccccggcggc
     6481 cggccgatcg agaagctgga ctacagcgac gaaaaggcgc tggctcagtt cgtccgctcg
     6541 cggagcatcg accgcgattt ctcgggcagc accatgccgc cgcccgaagc ggtgaaggcc
     6601 ggcaaggtgc agccgctgtc ggacgaggac cgccgcacga ttgtccgctg gatcgacctg
     6661 ggctgcccaa tcgacctcga cccgcggtac gacccggcgc aggccggcag ccgcagctac
     6721 ggttggatgt gcgacgacca gcggccgacg ctggcggtta ctttgccgcg agagggcggc
     6781 aacaaattgc tttcgcacat tctcatcggc atggccgacg cctacacggg cttggatcct
     6841 ggcagcttca ccgtcgtcgc cgatttcccg gtggatggcg ccgccgcggg cgaaaacctc
     6901 gcctcaaagt tcaccgccct accgggcaat cgctggcagc tgaagctggc caggccgatc
     6961 gacgacttgc cgcagggaac gctgacagtc tccgtgaagg acaagcaagg gaacattcag
     7021 cgtgtaaagc gggaattctc tgcaggacca agcctggctt catcgcagtg aattctgccg
     7081 caactgcaac tcgacaagcg tgccgtcctg cggcttggta gagacgccgg ctttggcggg
     7141 gttgccctga tggacggcct ggaagcggaa catcttcacg actgcgccga cgacgaggcc
     7201 gacttcgagc tggcccaggt gcttgccggg gcaaacgcgg gggccgtggc cgaagccgaa
     7261 gtgcaggatc tccttggagc cccggccctg ttcggccagg ttggtccaac gctcaggcac
     7321 gaattcgctc gcgggatagc cggtgaccgc cgcgccccag tggtcctcgt ggcggttggc
     7381 gtgccagacg tcgaggagga tgtgcgtgcc gcggggaatg aacatcttcc ggccgtcggc
     7441 ggtttccacc caggtatccg ccgtggcccg ccgcggcaga aagtagagcg agggagtcag
     7501 ccgcagggtt tcatcgagca cctgccccag gtagcgtgcg gcgtccaagt tgtcggggtt
     7561 gtagtcgtcc acgtccttca cttcctgata gaccttctcc tgggccgcct ggttccgcgc
     7621 caggtgcgcc agggcccaac tggcgaagga ggtcgtcgcc tccagggcgc ccgccaggaa
     7681 caccttgagg ttgctgcgga gcgccgcgtc cggtgcgtcg gacttgaatt gcctccagag
     7741 gcccttgcct tcctttcgcg gcgcgagcac gagggtcgtc agctcgtcga agcaggcgta
     7801 gtcggctttg gtctgtgcga tccgccgcgt gaagctcggc agccacgaga tcggaagacc
     7861 cagcttgttc gtcacggtat cgcttacgat gtggtcgatc acccgctcca gggccggaac
     7921 atagcggttt cgcacctgct cgtaactcaa ttcggcgcta aagaagttgt tggccagcat
     7981 ctccagcatt accgccttga tctccggctc cagcgggacg cggaccgacg acttgccgct
     8041 gctttgcagg tgctcgcgga gcacgcccag ccgccgcacg atcgtgccgc gaaacgtggc
     8101 ggcgaactcg tggaacatct ccggctgaaa gagcgtcgtc ttgccgaacg gcgaggcggc
     8161 aagcttccgc tgccgccgcc agagcggccc attggcgaag agcagcgtgt ccgagccagt
     8221 cgcccgggcg atgccggtcg aaggcagcgt gtcgcggtcg aattgcccgt cgcggtcgcc
     8281 cgtttccgcc gcgatcgcgc ggatcatcgc gggatcgcgg gtgaccagga tcggcgcaca
     8341 tcccggtatg tcgagatagc ggttgtggcg gcccgggccc gtctcgctct ccgcgcccca
     8401 gaagtacgac tccagaatgc ggatcggctg gtcgtagttc cagggatgcg gaaagggcag
     8461 cgttggccgg cccgaaccgg gctgaaagag ccgcacgcgg ctgggccggc tgtcgccgtc
     8521 aaacgggctg attgcaaaca gctgccccgg caggcgcatt agccgatgcc agagcgggac
     8581 ataatcggcc aataatcgcg cgagtcttcc gggcatggag tcgggagtcg gggagtcggt
     8641 cggtcggtgg tcggtaagtc ggtggtcggt ttccatgctg agtactcagt acgaatacca
     8701 cgcaccacca ctcatcactg acctaccgac tatcggctac cga
//
LOCUS       DASRJB010000915         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2796456, whole genome shotgun sequence.
ACCESSION   DASRJB010000915 DASRJB010000000
VERSION     DASRJB010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796456"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFB80_24075"
     CDS             <1..704
                     /locus_tag="VFB80_24075"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZN36938.1"
                     /translation="LALAFTQIPTHEQIIGQIKRTLAANQMTDGVHIRLTLTRGVKVT
                     SGMDPRLNQAGPTLIVLAEHKPPVYDKAGLSLVTSSVRRFPPDCLDPKIHHNNLVQSI
                     LAKIEANVAGADDALMLDTRGFVAETNATHLFLVSRGELATPRTVACPEGITRAVVLE
                     ICRQQSVPCSERDLSLTEFYRADEVFCTGTMGELAPVTKIDGRPIGNGQPGPMTRRLS
                     EMFAERTRSEGVPVA"
     gene            1466..>2847
                     /locus_tag="VFB80_24080"
     CDS             1466..>2847
                     /locus_tag="VFB80_24080"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZN36939.1"
                     /translation="MSRLALAFALGTVAAAAVSHAADIKTNNEPAQLDIRAAGEHSIR
                     VTLKPLSFQPEFPFTPALAEREYAAPVIRLREISESVKARVGGIDVEVRPDPLTIVAS
                     AAGKKIQTLTFQEDGSLAFQLGGQPVLGMGEGGPLPRGNFRTLPIEFDRRGRLEDMRP
                     RWQSDAYGSRNPVALMIGTEGWGLFIATPWGQVDLRDTGRGVFTPWQPPDPAAVPQRG
                     KGRQSYTAQVQGRPPVESLVKGAYDLFVCDAHEPAKFMKDLSVISGPAVLPPKWALGY
                     MQSHRELVDRNLSSEALILNVVDTFREKRIPVDAVIYLGTGFTPTGWNTRQPSFTFNP
                     RVFERDPKQVLADLHAKHVKVVTHIVPYQRDRLPSLQGTIPAKQGATLDDSHIQNYWQ
                     QHVELQTAGVDAWWPDEGDWFNQFERIKRHQLYYQGPLSTRPGERPWSLHRNGHLGVA
                     QWGGWVWSGDT"
BASE COUNT          519 a          942 c          910 g          476 t
ORIGIN      
        1 ctctggccct cgccttcacc caaattccga cgcacgagca aatcatcggc cagatcaagc
       61 gcacgctggc cgccaatcag atgaccgacg gcgttcacat ccgcctgacg ctgacccgcg
      121 gcgtgaaagt caccagcggc atggacccgc ggctgaacca ggccggtccg accttgatcg
      181 tgctcgccga gcacaagcca cccgtttatg acaaagcggg cctgtcgctc gtcacgagca
      241 gcgtccgccg ctttccgccg gattgtctcg atcccaaaat ccaccacaac aacctggtcc
      301 agtcgatcct ggccaagatc gaggcgaacg tcgccggggc cgacgacgcc ctgatgctcg
      361 acacccgcgg cttcgtcgcc gagaccaacg cgacacacct gttcctcgtc agccgcgggg
      421 agctggccac gccccgcacc gtcgcttgcc cggaaggaat tacgcgggcc gtcgtgctgg
      481 agatctgccg ccagcaatcg gtcccctgct ccgagcgcga cctttcgctg accgagtttt
      541 accgggccga cgaagtcttc tgcaccggca cgatgggcga gctcgccccg gtcacgaaga
      601 tcgacggccg cccgatcggc aacggccagc ccggaccgat gacccgccgg ctgagcgaga
      661 tgtttgccga gcggacgcga agcgaaggcg tgccggtcgc ttgagtcggt gctcgtggac
      721 tcttacctga cgctgcgccg ctttcacggc cgacttccaa cgctgggcat cttcaaatct
      781 cccgcgctcc cccggccgtg ggagctctga aatggaccaa cctagtccat ttctcggcag
      841 gcgcaaaaaa aagtttttca cgacttacgt cgagcgcgga ttgtgtaatc ctgtacccta
      901 tatacgcttg cggcgatgcg cggcgccggc agtttaataa aaatgtacac aagttgcttt
      961 tcagcgctca atcggcttgc taaactgctc ggacgatgtg gactcccggt gcgccgtggc
     1021 ggatgggcct tcgtccttcc gacgcaggcg cggcaggctg ccaggcgcag cacgaggaag
     1081 aatggcccgg actcgtcgaa ctgggccggc gggagctctg gaagcggcgg ctggctgctg
     1141 gaggggcggg agtctttggc cccggcaagc cgactctacg gcagccgcgc gcggaagccg
     1201 ctagaccgcg accaatggtc gcggctttga ggctcatgcg ggcggcgacc gcgttctcta
     1261 agtgtaataa gtagtattta acggcctgcg gctgcctttg ccgcaagcca atggcacatc
     1321 aggagttgcg accgaaaaat gcggaaaaag gcgcgttcgc cctcgcccaa ccagggggct
     1381 aataatgaag gccgcgcgta gcataagctc ctcgcttgtg gacgcttgct ctacggtttc
     1441 gtgtaatcgc cgcaaggagc gaatcatgtc caggctcgca ctcgccttcg ccctcggcac
     1501 ggttgccgct gccgccgtca gccacgccgc cgatatcaaa accaacaacg agccggcgca
     1561 gcttgacatt cgcgcggcgg gcgagcacag catccgagtg acgctgaagc cgctcagctt
     1621 tcagccggag tttccgttca caccggcgct ggccgagcgg gagtacgccg cgccggtgat
     1681 tcgattgcgg gagatcagcg agtcggtgaa ggcccgcgtc ggcggcatcg acgtcgaagt
     1741 ccgtcccgac ccgctgacga tcgtggcatc ggccgccggg aagaaaattc agacactcac
     1801 gttccaggag gacggcagtc tggcgtttca gctcggcggc cagccggtgc tgggaatggg
     1861 agaaggcgga cccttgccgc ggggcaactt ccgcacgctg ccgatcgagt tcgaccgtcg
     1921 cggccggctc gaagacatgc gcccgcggtg gcagtccgat gcctacggct cgcgcaaccc
     1981 ggtcgcgctg atgatcggca ccgaaggctg ggggctattc atcgccacgc cctggggtca
     2041 ggtcgatctg cgcgataccg gacgcggtgt gttcacgcct tggcagccgc ctgatccggc
     2101 ggcggtcccg cagcgcggca aagggcggca gtcctatacc gcgcaggtgc aaggccgccc
     2161 gccggtcgag agcctcgtca aaggcgcgta cgacctgttc gtgtgcgacg cgcacgagcc
     2221 ggcgaagttc atgaaggacc tctcggtcat cagcggcccg gccgtgctgc cgcccaagtg
     2281 ggcgctcggg tacatgcagt cgcaccgcga gctggtcgat aggaacttgt ccagcgaggc
     2341 cctgatcctg aacgtcgtcg atacgtttcg cgagaagaga atccccgtcg atgcggtcat
     2401 ctatctcggg actggattta cgccgaccgg ctggaacaca cggcagccct cgttcacgtt
     2461 caacccgcgg gtcttcgagc gcgacccgaa gcaagtgctc gccgacttgc atgcgaagca
     2521 tgtgaaggtc gtcacgcaca tcgttccgta tcagcgcgac cggctgccga gcctgcaggg
     2581 cacgatcccg gcgaagcagg gcgcgacgct cgacgactcg cacatccaaa actactggca
     2641 gcagcacgtc gagctgcaga ccgcgggcgt cgatgcctgg tggcccgacg agggcgactg
     2701 gttcaaccag ttcgagcgga tcaagcggca tcagctttat tatcaggggc cgctttcgac
     2761 gcggcccggc gagcggccct ggagcctgca ccgcaacggc cacctgggcg tcgcccagtg
     2821 gggcggctgg gtctggtcgg gcgacac
//
LOCUS       DASRJB010000916         9309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_564279, whole genome shotgun sequence.
ACCESSION   DASRJB010000916 DASRJB010000000
VERSION     DASRJB010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564279"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1876)
                     /locus_tag="VFB80_24085"
     CDS             complement(68..1876)
                     /locus_tag="VFB80_24085"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN36940.1"
                     /translation="MPDLIAQGAEPQQRWRRPLSLDVPHVIGRSAGPWAVPWDERISR
                     HHVEVRLAGAKLRVSVLDSARNPVFFRGRKTDEFEIRPGEHFVIGGTTFTLADERVHV
                     SLEVPLPEHERTFTVEELRRRPYEQADQRIDVLSRLPDIISGSSTDNEMFVRLVNLLL
                     SGVPRAAAAAIVAVPSSIVAVGLNPEPRTLNPVSVFHWDRRILSGTEFRPSERLIRRA
                     VESGESVAHVWSGEKPRGGEYTLSEGVDWAFCVPIPGDSCPGWSLYVTGGFDADALAA
                     HRGDPESLRDDIKFTELTAATLSALRQSRLLAGRQASLAQFFSPVVLSALEGRDPEEA
                     LAPREADVTVLFCDLRGFSRHSERHADHLLELLQRVSQALGVMTRHILDEGGVVGDFH
                     GDSAMGFWGWPLPQSNAVERACRAALAIRSQFATFSADSTPPALADFRVGIGIASGRA
                     VAGKIGTVDQVKVTVFGPVANLAARLEGLTKVLRVPILMDAATAATVRAGVPREVARL
                     RRLAKIKPPGMETAVEVSELLAPAAEYPQLTDEHLAAFESAVEALYARDWAGALQLLH
                     RVPPDDLAKDFLTVFIAQHNRTPPADWDGIVAIPTR"
     gene            1993..2547
                     /gene="cyaB"
                     /locus_tag="VFB80_24090"
     CDS             1993..2547
                     /gene="cyaB"
                     /locus_tag="VFB80_24090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HZN36941.1"
                     /translation="MQLEVEQKFPMREPATVRQSLVELGATFGTPIKQADVYFSHPSR
                     NFKETDEALRIRRVGQDNFVTYKGPKLDPATKTRREIELPLAAGDAGFAQFAELLEVL
                     GFRRVYEVRKHRIPGEIPWEGRTIHLALDDVTGLGSYLEIELLADEKELGGAKACLAS
                     LAAAVGLTGQERRGYLDLLLSGSA"
     gene            2784..3416
                     /locus_tag="VFB80_24095"
     CDS             2784..3416
                     /locus_tag="VFB80_24095"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HZN36942.1"
                     /translation="MKRHLAWVVMMVLAASPSLAEDGKAKNPAGKRPAEGAGGPLNNP
                     ELMKRLLEKFDENKDGRLSPQEQAKAQEEFLKGAAQAGGPAFQEFLKKFDRDGDGKLN
                     DRERAAAMEAAQKIRASGGGGGPQVGPPLTFAGNEIPAAILEKFDKDGDGKLNDEEMA
                     LAKKAQSGKRERAKKSDVIKEFDKDGDGKLNDEEKAAAKAAAKERKKKAE"
     gene            3642..8036
                     /locus_tag="VFB80_24100"
     CDS             3642..8036
                     /locus_tag="VFB80_24100"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN36943.1"
                     /translation="MKFRLEKSDGTWRWFGSLAIFLALAQPCAAQDGDDEQPYLPGLL
                     ATYEDASARQARRLDDAIAFDWKETLPDPRLSKSAFTSRWQGLLLAQGAGEYRLHVFA
                     TGQVEVRLAGKVAIQRQRLKEAWAVSQPLTLSFDHHPLEVEFQQTEASARIALFWSGP
                     QFALEPISPRSFWHDRKATVSSDFERGRLLAAALRCGACHQHEASEPVMPAPSLANLA
                     GNIQPQWLIDWLMESGHETKELDAGQPLPALRRMPSLGLPRDDAEAIAAYLLPPGEKR
                     PKPKPDSANAADERKAEQAGPSAEDENGEKQTSNKKKEAKKEPPSAKQGERLFLTLGC
                     LSCHQWGKLGESGLFGGGDLSQVGDKRPADFFARWLADPAAINRRHRMPVFELTGDER
                     TSLSLFLAEQRTKQRDAPARLDASAKIASRGRELALKLRCGACHELPEMPAKPAPIAT
                     VKLDDRFDWSRSCASARSESKTGLGYGLLPEQQRQLETFFRGSNQQGQIDSPPDGDML
                     LVTHNCLACHARDGQVAIASALPAKLADKLAAVGQAHDELAPLIPAMTPPSLNSVGDK
                     LHDEALAAAIRRSTAPYRHYLLVRMPRFRLSDQELAALTRHFVTADRIPAGGAAVAQR
                     PAVQEPALAAAGGRLVSTDGFSCVSCHQIGSVVPDKAPLNARGPGLSQIDQRIRREWF
                     DRFVADPSRIVPRMEMPSVRIPVKGVLGDKVDAQLAAVWHVVNLPLFEPPEPNPVRVL
                     RRSGIAERNEPPIAISDVVKIGERTIVHPLVIGLPNRQNFLYDLSEGRLAAWWLGDAA
                     RQRTKGKSWHWEPGGPMVMPAADGGAELSLIRGGKRLVPLRNGQHHATLSGYKLAEGG
                     VQATYRMNFGDPERPAAPTIAVEISDHWLPGRDAASWRTESLRVLKIVPALAPDEQVS
                     IAVQLAPGNAKWERSADGRSVRMIEAPRAEIVLHGRSEGGWTLSENGTATSVPTTAQH
                     RGMYLVLHYRSGLTPDTLDAPEPVFPADVGPVKVAPGFTGRRLPLPAGLMPTGLAWKS
                     AAGEPQLLIGSLKGQVIAARDSDSDGLEDAVRVVADGLATPYGIADDGDAVHVLTKAA
                     LLEVKDGGVKVLASGWGHSDDYHDWAVGLPRNQQGEYFIGLPCQQDKRSAAAAKFRGA
                     VLKLTPRMPTGDNPQRYDLHVVSTGHRFPMGLALDRRGELFVTDNQGNYNPFNELNHV
                     RQAAFFGFINANEKKDNGFQAPPLTEPAIDIPHPWTRSVNGICFLDTPQPLRKEGKSL
                     FGPLEGHLVGCEYDTRRLVRMTLQQVGDTYQGAAYPLSLEPSSPEKGFLGPVVCALSP
                     RGELYVGSMRDSGWGAGNNVGEVVQVRIAPDELPAGIGQVRAIAAGFEVDFIRQVDVA
                     KAGDSANYSISSYRRESTPAYGGPDLDRRIEKVTGIAVSPDRRRVALKLAELREGFVY
                     EFQLKSLTGDGGVFHPAEAHYTLRKRPD"
     gene            8189..>9309
                     /locus_tag="VFB80_24105"
     CDS             8189..>9309
                     /locus_tag="VFB80_24105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN36944.1"
                     /translation="MIGRFLSLAGVTWAMALAVSAVTIDVASAEVVVLKSGGRIEGEL
                     VNRDRARTDPLIIVDETGVRLSLSPSQVERVVVKKDVEKQYEERLPKIPNTAAGHWEM
                     AAWCQEAGLSTQRKTHLEEVIRLDPNHEAARAALGYSRIGEAWMKPDEWMRKQGYVYY
                     EGRWCLPHEVEIDKREREWELATKKWRKDIKTWIGWVEDNGRKSVAGMENLRGIRDEA
                     AAPALVEVLTSDNLPRSLRMQSLELLSRMPATYSTSTLITMAMKDRDEEMRDKSLDEL
                     RRRRSTQALHAFIKDLKSKDNTVVRQAAHCLAVLGDPEATVPLIDALVTSHKFAVTQG
                     GSPGQLGVGFGGPTDGSGGGGGSFNFGTPKPQIVTRKIE"
BASE COUNT         1755 a         2885 c         3163 g         1506 t
ORIGIN      
        1 aacagcggag ccgccatgag ctcttcgcta atggcatgtc attggacatg tgtgtcctct
       61 cgacagctta ccgcgtgggt atcgccacaa tcccgtccca atccgccggc ggcgtccggt
      121 tgtgctgggc gatgaagacg gtgagaaaat ccttcgccag gtcgtcgggc ggaacgcggt
      181 gcaaaagctg caaggcgcca gcccagtcgc gggcatacag agcctcgacg gccgactcga
      241 atgccgccag gtgctcgtcg gtaagctgcg gatactcggc agcaggcgcg agcagctcgc
      301 tcacttccac cgccgtctcc atgcccggcg gtttgatttt cgccagccgc cgcaggcggg
      361 ccacctcccg cggcacgccg gcccggaccg ttgccgcggt cgccgcgtcc atcaggatcg
      421 gcacgcgcag caccttggtc agcccttcca gccgcgctgc aagattggcc accggcccaa
      481 agacggtcac tttcacctga tcgaccgtgc cgatcttgcc ggccacggcc cgcccgctgg
      541 cgatgccgat gccgacgcga aaatcggcca gtgccggcgg cgtcgaatcc gcgctgaacg
      601 ttgcgaactg cgagcggatc gccaaggccg cccggcaggc gcgctcgacg gcattgcttt
      661 gcggcagcgg ccagccccaa aagcccatcg cgctgtcgcc gtggaaatcg ccgacgacgc
      721 ccccctcgtc gagaatgtgc ctggtcatca cccccaaggc ctggctgacg cgctggagca
      781 gttccagcag gtggtcggcg tgccgctccg aatgccgcga gaagccgcgg aggtcgcaaa
      841 acaggacggt gacgtcggcc tcgcggggag cgagcgcttc ttcgggatcg cggccttcga
      901 gcgcggagag gacgacggga gaaaagaatt gtgccagcga tgcctggcga ccggccagca
      961 gccgcgactg gcgcagcgcg ctgagcgtgg ccgcggttag ctcggtgaac ttgatatcgt
     1021 cgcggagcga ttccggatcg ccgcggtggg cggccagcgc gtcggcgtcg aagccgccgg
     1081 tgacgtacag cgaccagccg gggcaggaat caccgggaat gggaacgcag aacgcccaat
     1141 cgaccccttc gctgagcgtg tattcgccgc cgcggggctt ctcgccgctc cacacgtgcg
     1201 cgacgctttc tccggattcc acggcgcgac gaatcagccg ttcactgggg cggaactccg
     1261 tgccggagag gatgcggcgg tcccagtgga agacggagac agggttcaag gttcggggtt
     1321 cagggttcag cccgacagcg acaatcgatg acggaaccgc cacgatggcc gctgccgctg
     1381 cccgcggcac tccggagagg agcaggttca cgagccgcac gaacatctcg ttgtcggtgg
     1441 agctgccgct gatgatgtcg gggagtcggc tgaggacgtc aatccgctga tcggcctgct
     1501 cgtacggacg gcggcgcagc tcttccacgg tgaaagtccg ctcgtgctcg ggcaacggca
     1561 cttccagaga aacgtgcacg cgctcgtcgg ccagtgtgaa cgtggtgccg ccaatgacaa
     1621 aatgttcgcc gggccggatc tcgaattcgt cggtcttgcg gccgcgaaaa aaaaccgggt
     1681 tgcgggccga gtcgagcacg ctgacgcgca atttggcgcc ggccaggcgc acttccacat
     1741 ggtggcgcga gatccgctcg tcccagggga ctgcccacgg gccggcgctg cggccgatca
     1801 cgtgcggcac atcgagagat aatggacgcc gccagcgctg ctgcggctcg gcgccctggg
     1861 caatcagatc aggcatggaa agctcggcca gcggggatga ggtattatag gagtgcggaa
     1921 tgcagagtgc agaatgcagg tggcggccag tttcccggtt ccgaattcgg tccgctgaac
     1981 gctgagtcca tcatgcagct cgaagtcgaa cagaagttcc cgatgagaga gcctgccacc
     2041 gtgcgacagt cgctggtcga actgggggca acattcggca cgccaatcaa acaggcggac
     2101 gtctatttct cgcatcccag ccgcaatttc aaggagacgg acgaagccct acggattcgc
     2161 cgcgtcggcc aagataactt cgtgacgtat aagggtccca agctcgaccc tgcgaccaag
     2221 acgcggcggg agatcgagct gccactggct gccggcgacg cgggatttgc acagtttgca
     2281 gagctgctcg aagtcctcgg atttcgccgc gtgtacgaag ttcgcaagca ccgcatcccc
     2341 ggcgaaattc cttgggaggg tcggacgatt catttggcac ttgatgacgt gacggggctg
     2401 gggagctatc tggagatcga gctgctggcc gacgagaagg aactaggcgg cgccaaggct
     2461 tgcctggcgt cgctggccgc cgctgtggga ctgaccggtc aggagcggcg cggctacctg
     2521 gatttgctgc tttccgggtc cgcgtaagcc gttagggtgt caacgtttgc gacttccatt
     2581 tcgcttggcg cgcggtccgg ccgcttgatg agggtcgatt tttcgcccat gaacccgaac
     2641 gattgactag agtaaatttc tcgtttgccg gcgaaacctg acgggcagcg ccggggtttg
     2701 ttcctctgat cggtggttcg gccacagcgg ggtcgctctg cggggcgtgg tcggctggcg
     2761 gtcgcatcgc ggtggagaga gtgatgaaac ggcatctcgc ttgggtggtg atgatggtgc
     2821 tggcggcgtc cccgtcgctg gcggaagacg ggaaggccaa aaatccagcc ggcaaacggc
     2881 ctgccgaggg agccggcgga ccgctgaaca atccggagct gatgaagcgg ctgctggaga
     2941 aattcgacga gaacaaagac ggccgcctca gccctcagga gcaagccaag gcacaggagg
     3001 aattcctcaa aggcgccgcc caggctggcg gcccggcatt ccaggagttc ctgaagaaat
     3061 ttgatcggga cggtgacggc aagctgaacg accgcgagcg ggcggcggcc atggaagcgg
     3121 cccagaagat tcgcgcgtcg ggcggcggtg gtggaccgca ggttggaccg ccccttacgt
     3181 ttgctggaaa tgaaatcccc gcagcaatcc tggaaaagtt cgacaaggat ggcgacggca
     3241 aattgaacga cgaagagatg gccctggcca agaaggcgca gagcggcaaa cgagagcgag
     3301 ccaagaaatc ggacgtcatc aaggaatttg acaaggacgg cgacggcaaa ctgaacgacg
     3361 aggagaaggc agcggccaaa gcggccgcca aggagcgaaa gaagaaggcg gaatagcgct
     3421 cgaatgaaaa tggcgcgcga tctttctgcg ggttaaactt gcccccaagc gaaggcccgg
     3481 cgtcggcgca ctcttatggc cgacgaccgg gcctctttat tttcttgccg ccgtcagtga
     3541 atctggggcg cgtccttctt acaatcggac gtgtgctctc tgttgtccgg ttcgccagag
     3601 ttgacattcg ccagcaccag ttgcagagca atcatcgcgt gatgaaattt cggctcgaga
     3661 aaagtgacgg cacatggcgc tggtttggca gcctggcaat ttttctcgcg ctggcccagc
     3721 cgtgcgctgc gcaggacggc gacgacgaac agccgtacct gccggggctg ttggcgacgt
     3781 acgaggatgc ttccgctcgc caagcgcgcc gactggacga cgcgatcgcg ttcgactgga
     3841 aggagacact ccccgatccg cggctgagca agtcggcgtt taccagccgc tggcagggat
     3901 tgctgctggc gcagggcgcc ggcgagtatc ggctgcacgt ttttgccacc ggccaggtcg
     3961 aagtccgtct ggcggggaaa gtggccatcc agcggcagcg actgaaagag gcgtgggccg
     4021 tttcgcagcc gctgacgctt tccttcgacc accatccgct ggaagtggaa tttcagcaaa
     4081 ccgaggcgtc ggcacgcatc gccctcttct ggtccgggcc gcagtttgcg ctcgagccga
     4141 tttcgccgcg gtctttttgg cacgaccgca aagcgacggt gagcagtgat ttcgagcgcg
     4201 gccggctgct ggcggcggct ctccgctgcg gggcatgtca ccagcacgag gcgagcgagc
     4261 ccgtcatgcc cgcgccgtcg ctggcgaatc tggccgggaa tattcagccg cagtggctga
     4321 tcgactggct gatggaaagc gggcacgaga ccaaagaatt agacgccggg cagccgctgc
     4381 cggctctgcg gcgcatgccc agcctcgggt tgccgcgcga tgacgcggag gcgattgccg
     4441 cctacttgct gcctcccggc gagaaacgac ccaagccgaa acccgattcc gctaatgccg
     4501 ccgatgagcg aaaagccgag caagccgggc cgtccgcgga ggatgagaat ggcgagaaac
     4561 agacgagtaa caagaagaaa gaagcaaaga aggaacctcc cagtgccaaa caaggtgagc
     4621 ggctgttttt gacgctcggt tgcctgtcct gccatcaatg gggaaaactg ggtgagagcg
     4681 gcctgttcgg aggcggcgac ttgtcgcagg tgggggacaa gcggcctgcg gacttcttcg
     4741 cgcggtggct cgccgacccg gccgccatca accggcgcca tcgcatgccc gtgttcgaat
     4801 taaccggcga cgagcggacg agcctgtcgc tatttctggc ggagcaaagg acgaagcaga
     4861 gagatgcacc agccagactc gacgccagcg caaaaattgc cagccgcggg cgggagctcg
     4921 ccctgaagct ccgctgcggc gcgtgccacg aattgcccga aatgccggcc aagccagcgc
     4981 cgatcgccac ggtgaagctc gacgaccgct tcgactggtc gcggtcgtgc gcgtcggcac
     5041 gcagtgagtc gaagaccggt ctgggctacg ggctgttgcc cgagcagcag cgacagttgg
     5101 aaactttctt tcgaggatcg aatcagcagg gccagatcga ttcgccgccc gacggagaca
     5161 tgctgctggt cacgcacaac tgcctggcct gccacgcgcg cgacggacaa gtcgcgattg
     5221 cgtccgcctt gccagccaag ttagccgaca agctggccgc cgttggccag gcgcacgacg
     5281 agctggcgcc gctcatcccc gccatgactc cgccgtcgct caatagcgtc ggcgacaagt
     5341 tgcacgacga ggcgctggcc gccgccatcc gccgcagcac agccccctat cggcactacc
     5401 tgctcgtgcg aatgccgcgg ttccggctaa gcgaccagga gctggccgcg ctgacgcggc
     5461 acttcgtcac ggctgaccgg attcccgccg gcggagcggc cgtggcccag aggccggcgg
     5521 tacaggagcc ggcacttgcc gccgctggcg gccgcctcgt ctccaccgac ggctttagct
     5581 gcgtgagctg ccaccagatc ggcagcgtcg ttccggacaa ggcgccgctc aatgcccgcg
     5641 ggcccggcct gtcgcaaatc gaccagcgaa ttcgccgcga gtggttcgac cgtttcgtcg
     5701 ccgatccgtc gcggattgtg ccgcgaatgg aaatgccgag cgtgcgaatc ccggtgaagg
     5761 gggtcctggg cgacaaggtc gatgcgcagc ttgcggccgt gtggcacgtg gtcaatctgc
     5821 ctctcttcga gccgccggag ccgaacccgg tgcgcgtcct ccgccgcagc ggcattgccg
     5881 agcggaacga gccgccgatc gcgatcagcg acgtcgtgaa aatcggcgag cggacgatcg
     5941 ttcatccgct cgtgatcggc ctgcccaacc ggcagaattt tctctacgac ctgagcgaag
     6001 ggcggctggc cgcgtggtgg ctgggggacg cggcacggca gcggaccaag ggcaaaagct
     6061 ggcactggga gccgggtggg ccgatggtga tgccggccgc agacggcggt gccgagttgt
     6121 cgctcattcg cggcggcaaa aggcttgtcc ccctgcgaaa cggccagcac cacgcgacgc
     6181 tatccggcta caagctggcc gaagggggcg tgcaggcgac gtaccggatg aatttcggcg
     6241 atcccgagcg tccggctgcg ccgacgatcg ccgtggagat atccgaccat tggcttcccg
     6301 gccgcgatgc cgcgtcgtgg cggaccgagt ctctacgcgt cctgaagatc gtccccgcgc
     6361 tcgcgccgga cgagcaagtg tcgatcgcgg ttcagctcgc gccaggaaat gccaagtggg
     6421 agcgctcggc ggacggaagg agcgtgcgga tgattgaggc gccgagagcg gagattgtct
     6481 tgcacggtcg ctcggagggc ggctggacgc tgtcggaaaa tgggacggca acgtccgtgc
     6541 cgacaaccgc gcagcaccgg ggaatgtatc tggttctgca ctatcgctcg ggcctgacgc
     6601 ccgacacgct cgatgctccg gagccggtgt tcccggccga cgtcgggccg gttaaagtcg
     6661 cccccggatt taccggccgc cggctgccgc ttcctgctgg actgatgcca acgggattgg
     6721 cctggaagtc agcggctggc gaaccccagt tgctgattgg ctccctcaag ggccaggtca
     6781 tcgcagcccg cgatagcgac agcgacggtt tggaagacgc tgtaagggtt gtcgccgatg
     6841 gactggcgac accctacggc attgccgatg acggcgatgc ggtgcacgtg ctgacgaagg
     6901 cggcgctctt agaggtcaaa gacggcggcg tgaaggttct cgcctccggc tggggccaca
     6961 gcgacgacta tcacgactgg gcggtcggtt tgccgcggaa ccagcaaggg gaatacttca
     7021 taggcctccc ctgccagcaa gataagcgga gtgccgcggc agccaagttt cgcggcgcgg
     7081 tgctcaagct gacgccgcgg atgcctacgg gggacaatcc gcagcgctac gacctgcacg
     7141 tcgtctccac cggccaccgc ttcccgatgg ggctggcgct cgatcgccgc ggcgagctgt
     7201 tcgtcaccga caaccaggga aactacaacc cgttcaacga gttgaaccac gtgcggcagg
     7261 cggcgttctt cggattcatc aacgccaatg agaagaaaga caacggcttt caggcgccgc
     7321 cgctgactga gccggcgatc gacattccgc acccatggac ccgcagcgtc aacggcattt
     7381 gctttttgga tacgccccag ccgctgcgca aggagggtaa gtcgcttttc ggcccgctgg
     7441 aagggcacct cgttggctgc gagtacgaca cgcggcggct ggtgcgcatg acgcttcagc
     7501 aggtcggcga cacttatcaa ggggccgcct atccgctgag cctcgagcct tcgtcgcccg
     7561 agaaaggttt tctcggtccc gtcgtgtgcg ccctctcgcc gcgcggcgaa ctgtacgtcg
     7621 gcagcatgcg cgacagcggc tggggggccg gcaacaacgt cggcgaggtg gtgcaagtca
     7681 gaatcgcccc cgacgagctt cccgccggca ttggccaggt gcgggcgatt gcagccggtt
     7741 ttgaggtcga cttcatccgc caggtcgatg tcgcgaaagc tggcgattca gccaactact
     7801 cgatttcttc ctatcgccgc gaatcgacgc cggcctacgg cggccccgat ctcgaccggc
     7861 gcatcgaaaa ggtcaccggc attgcggtct cccctgatcg acgccgcgtg gcgctgaagc
     7921 tggccgaact gcgcgagggg ttcgtttatg agttccagct caaaagtcta accggcgacg
     7981 gcggagtgtt tcatccggcc gaggcgcact acacgctgcg gaaacggccg gattagccgt
     8041 gcccgccgca cacggctatt ccaattgtgc cgattctttc gttgctgctg cgggcaaaac
     8101 gtgggtatac tagagggaca gcggcccgag ccaacaccag gggtcctagc gcacctaaat
     8161 gccgccagct cggccgcggg agcatcggat gatcgggcgt tttctatcgc tcgcgggcgt
     8221 cacttgggcc atggcgctag cagtctccgc ggtcacgatc gacgtggcta gcgccgaggt
     8281 agtggtcctg aaatcggggg gccgcattga aggggagctg gtcaaccggg accgcgcccg
     8341 caccgacccg ctgatcattg tggacgagac cggcgtgcga ctgtcgctca gtcccagcca
     8401 ggtcgagcgg gtcgtggtta agaaggacgt cgagaagcag tacgaagagc ggttgccgaa
     8461 aattcccaat acggccgcgg gacactggga gatggctgcc tggtgccagg aggcgggact
     8521 gagcacgcag cgaaagactc acctggagga ggtgattcgc ctcgatccga accacgaggc
     8581 ggcgcgggcc gcactgggct actcccgaat cggcgaggcg tggatgaagc ccgacgaatg
     8641 gatgcgcaaa cagggctacg tctactacga ggggcgctgg tgcctgcctc atgaagtgga
     8701 aatcgacaag cgggagcggg agtgggaact ggccaccaag aagtggcgca aggacatcaa
     8761 gacctggatc ggctgggtcg aggacaacgg ccgcaagtcg gtcgcgggca tggaaaacct
     8821 gcgcggcatt cgggacgagg cggctgcgcc cgccctggtg gaagtgctga ccagcgacaa
     8881 tctgccgcgc agcttgcgga tgcagtccct ggaacttctc tcgcggatgc cagccaccta
     8941 ttcgaccagc accttgatca cgatggcgat gaaggaccgg gacgaggaga tgcgcgacaa
     9001 gagcctggac gagctccgcc gccggcggtc cacgcaggcc ctgcacgcgt tcatcaagga
     9061 cctcaagagc aaggacaaca cggttgtgcg tcaggcggcg cattgcctgg cagtgctcgg
     9121 cgacccggaa gcgaccgtgc cgctgatcga cgcgctagtc acctcgcaca agttcgccgt
     9181 cacgcaaggc ggaagtcccg ggcagctcgg cgtcggcttc ggcgggccga ccgacggcag
     9241 cggcggtgga gggggcagct tcaacttcgg cacccccaag ccgcagatcg tcactcgcaa
     9301 gatcgaaaa
//
LOCUS       DASRJB010000917         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2243197, whole genome shotgun sequence.
ACCESSION   DASRJB010000917 DASRJB010000000
VERSION     DASRJB010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243197"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VFB80_24110"
     CDS             <1..1515
                     /locus_tag="VFB80_24110"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZN36945.1"
                     /translation="PDEGAGGEGFDNAAETLFLSPLHAEKFLEAARAALDYASKDPRS
                     RSTFLSTRPRGEGSGGGFGRGRRGGPPQPPEPPLTAEQAQTLLARFVPRAFRRPAREG
                     EIEQYQTLVTAAIERGESPEQALLFALQGVLMSPHFLFRLEEPNPTDQPRLVGQYEIA
                     SRLSYFLWASMPDEELLQGAGEGLLNDPESLRGQTERMLKDRKTREFAESFVEQWLGT
                     RELGRNVKPDRQANRYTNELEWALKQEPVLFFQYVLAEDRPLTELIDANYTFLDSKLQ
                     RHYRLEIKGTNQQLKRFDLPEGSHRGGLLGMGGVLAVTSMPHRTSPVLRGKWVKETLL
                     GSPTPPPPPDVPKLDEQPAADSPKTMRERLAAHRQNPTCASCHNHIDPIGFGLENYDL
                     LGRWRTEDAGQAIDALGELPGGVKFDGPEELKRVLLERKDEFVRHLTTKLLGYALGRG
                     LTVEDQCAVDEIVKNVKAGEYRSQVLIAEIVCSVPFRYRAGEGVTLKTSKTQGE"
     gene            1617..>2021
                     /locus_tag="VFB80_24115"
     CDS             1617..>2021
                     /locus_tag="VFB80_24115"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZN36946.1"
                     /translation="MQTPFPISRRTMLRGLGATLALPWLEAMRPRTALAADTPKQPVR
                     MAALYMPNGVNADQWTPKGEGREFELSPTLAPLAGLKSEILVLTNLWNKGSEASEGHY
                     VKTSGFLTCTTVTKTVGVDLSCNGVSMDQVAAR"
BASE COUNT          374 a          645 c          681 g          321 t
ORIGIN      
        1 cccgacgaag gcgccggtgg cgaaggtttt gacaacgcgg cggaaacgct gtttctctcg
       61 ccgctgcatg ccgagaagtt cctcgaagcg gcccgcgccg cgctcgacta tgccagcaag
      121 gacccgcgct cgcggagcac gtttctttcg acgcgcccac gtggcgaggg atcgggcggc
      181 ggctttggcc gcggccggcg cggcggcccg ccccagccgc cggagccgcc gctgacggcc
      241 gagcaggcgc aaacgctgct ggcgcggttc gtgccccggg cgtttcgtcg gccggcccgc
      301 gaaggggaga tcgagcaata ccagacgctc gtgaccgcgg cgatcgagcg cggcgaatcg
      361 cccgaacagg cgctgctctt cgccctgcag ggcgtcctga tgtcgcccca tttcctgttc
      421 cgcctggagg aaccgaatcc gaccgatcag ccgcgactgg tgggccaata cgagattgcc
      481 tcgcggctct cctatttcct gtgggccagc atgcccgacg aggagctgct gcagggggct
      541 ggcgaggggc tgctcaacga tcccgagtcg ctgcgcgggc aaaccgagcg gatgctcaag
      601 gaccgcaaga cccgcgagtt cgccgagagc tttgtcgagc aatggctggg cacccgcgag
      661 ctgggccgca acgtcaagcc cgaccggcag gccaaccgtt acacgaacga gctggaatgg
      721 gccctcaagc aggagccggt gctgttcttt cagtacgttc tggccgagga ccgcccgctg
      781 acggagctga tcgacgcgaa ctacacgttt ctcgacagca agctccaacg acactatcgg
      841 ctggagatca aaggcaccaa ccagcagctc aagcggttcg atctgccgga ggggagccat
      901 cgcggcggcc tgctcggcat gggcggcgtg ctggcggtca cttcgatgcc gcaccgcacg
      961 agccccgtgc tgcggggcaa gtgggtgaag gagaccctgc tgggcagccc cacgccgcca
     1021 ccgccgcccg atgtgcccaa gcttgacgag cagcccgcgg ccgattcccc caagacgatg
     1081 cgcgaacggc tggcggcgca ccgccagaac ccgacctgcg cctcgtgcca caatcatatt
     1141 gatccgatcg gtttcggcct ggagaattac gatctgctcg gccgctggcg gaccgaggac
     1201 gccggtcagg cgatcgacgc cctgggcgaa ctgcccggcg gcgtgaagtt cgacgggccg
     1261 gaagaattga agcgggttct gctggagcgg aaagacgaat tcgtccgcca cctgacgacg
     1321 aagctgctgg gctatgccct gggccgcggc ctgaccgttg aggaccagtg tgccgtcgat
     1381 gagatcgtga agaacgtcaa ggccggcgag tatcgctcgc aggtcctgat tgccgagata
     1441 gtctgtagcg tgccgttccg gtatcgcgcg ggggaaggtg taactcttaa gacttccaag
     1501 acacaaggag aatgaacaga aggtaacgaa ggaaacgaag cagagagttt atcccatccc
     1561 tcttcgtttc cttcgtttcc ttctgttcaa ttgcttggct acacgcagag gccatcatgc
     1621 agaccccgtt tccgatcagc cggcggacga tgttgcgagg gctgggcgcg actttggcct
     1681 tgccgtggct ggaggcaatg cggccccgaa cggcgctggc cgctgatacg ccgaaacagc
     1741 cggtgcggat ggccgcgctc tatatgccca acggcgtgaa cgccgatcag tggacgccga
     1801 aaggggaagg ccgcgagttc gagctgtcgc cgacgctcgc cccgctggct ggcctcaaga
     1861 gcgaaatcct ggtgctgacc aacctctgga acaagggcag cgaggcgtcg gagggacact
     1921 acgtcaagac gtccggcttc ctgacctgca cgacagtgac aaagacggtc ggcgtggacc
     1981 tgagctgcaa cggcgtgtcg atggaccagg tcgccgcgcg g
//
LOCUS       DASRJB010000918        15999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3939622, whole genome shotgun sequence.
ACCESSION   DASRJB010000918 DASRJB010000000
VERSION     DASRJB010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3939622"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFB80_24120"
     CDS             complement(<1..350)
                     /locus_tag="VFB80_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36947.1"
                     /translation="MMQPGARQLVSLITRTIALLFIAAALLLVLIALTYGVTEVGATG
                     LLLLGIAAALVIGYGLRLAWPSLLRAGRRIPVRFGLRTLLIMTLACGVSLAWLGNKVR
                     DMREQRQILRQVFAG"
     gene            complement(387..1280)
                     /locus_tag="VFB80_24125"
     CDS             complement(387..1280)
                     /locus_tag="VFB80_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN36948.1"
                     /translation="MIYRILIAAAAVALLLPAAACAQESGKLKALIVDGQNNHGNWPQ
                     TTKMMKKYLEETGKFTVDVATTAPRGTDASFKPEFKKYAVVVSNYNGADWPKETQAAF
                     IDYVKGGGGLVIVHAANNAFGKWKEYNEIIGLGGWDGRNETSGPYIYLDAEGKLVKSE
                     ERGGGGHHGAQHAFGLIVRDAEHPVTKGMPREWLHVNDELYDKLRGPGANMHILATAY
                     ADPAKGGTGRHEPMIFTVDYGKGRVFHTPMGHGNDSQECVGFITTLVRGAEWAATGKV
                     TLPIPSDFPTKEKTSRRKFEG"
     gene            complement(1349..3568)
                     /gene="selD"
                     /locus_tag="VFB80_24130"
     CDS             complement(1349..3568)
                     /gene="selD"
                     /locus_tag="VFB80_24130"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZN36949.1"
                     /translation="MPTHDVVLLGIGHTNAHVLRMWRMQPIPDARLTCVSNFPTATYS
                     GMLPGVLAGQYPPEAMEIDLVRLCAAAGARLILSNVTGLDRQHRELLFDDRPPLRYDV
                     LSVGIGSVPSRDGLQVADNTLLPIKPMQTFVARLESRVLASGRRRPPDGAIYRAVIVG
                     GGVGGVEIAFCLSSRLRTLLAERPFEITLVNSHPRLTPGTTDATAQRVQRELESRGVN
                     VILDRRVTSIEASTVTLDNGQSLAADLILWATSAAAPPLLGRVGLPTDDDGFLLTTPA
                     LQSTADPAVFAVGDSGTIVGQELPKAGVYAVREGPILWENIQRALRGQPVVTYRPQRG
                     FLKLINTGDGRAIGEYRGFTFAGRWAWRLKDRIDRKFMAMYQDYTPMLMGQGSGRPEM
                     DPPQMRCAGCGGKIGGTVLSRALSRLEIPLHDSLLVGLSQPDDVALVRVQDGQPLAAT
                     TDFFAAPLDDPYLVGRMAALHAASDVLAKGARPVAALAIATIPEGPAEQQEQLLFELL
                     AGGVRELKAMGATLAGGHTIEGPQLTIGYTILAEQARPLRAKGNLRAGDALILTKPLG
                     TGVLLAAHMQALLKAGCWQPLLNAMLRSNQPAAELLAEFDVRAVTDVTGFGLAGHLLE
                     MLRASKVAATLRLDSVPLLPGAAELLSGGMQSTLAPANAAAEREMDTAKCNRSSAGYA
                     ALFDPQTCGGLLIGVAREQAGELCRGLHAGGDEAACIVGEVATETGGRRLTILNSAF"
     gene            complement(3583..3987)
                     /locus_tag="VFB80_24135"
     CDS             complement(3583..3987)
                     /locus_tag="VFB80_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN36950.1"
                     /translation="MAKDKEKAKDEPAKTGKRVLLVDDDPEIIDAIRYALESKGFKIF
                     IARDGNQGLAMAESEDPDLVILDMMMPKRSGFLVLEKLRRTRPVPVRVIMITANEGSR
                     HKAYAEMLGVDDYLRKPFAMDKLVEAVQRLLA"
     gene            4102..4863
                     /locus_tag="VFB80_24140"
     CDS             4102..4863
                     /locus_tag="VFB80_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZN36951.1"
                     /translation="MTSRFTADELAQYGRDGYYIVRGIYDRGEIDKLLRFAKDDPSFA
                     GSVYGRKDAAGHETKLALWNFAGDDLYSMFARSPRIVDRMEQALGGEVYLYHMKMMLK
                     EPRVGGAWEWHQDYGYWYHNGCLLPLLASCLIAVSRANQANGCLQVLRGSHLMGRIDH
                     GKTGDQTGADMERVNVALERMPLDYVECEPGDAIFFDSNLLHRSDMNGSDEPRWSLIC
                     CYNARRNDPYKDSRHPRYTPLPKVADDAVRRWEPK"
     gene            complement(4870..5340)
                     /locus_tag="VFB80_24145"
     CDS             complement(4870..5340)
                     /locus_tag="VFB80_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN36952.1"
                     /translation="MSAEAKLVELKLQLPPLPKPGGIYKPVVTVGNLAYVSGHVSLDP
                     QGPQGKILTGRVGAEVDEAGGKKAAEAAGLAILATLRANLGSLDKVKRVIKTLGMVNS
                     TADFTNHPEVINGCSDLFAKLWGQDHGIGARSAVGMGALPRNATVEVEVIFELA"
     gene            complement(5422..5802)
                     /gene="hisI"
                     /locus_tag="VFB80_24150"
     CDS             complement(5422..5802)
                     /gene="hisI"
                     /locus_tag="VFB80_24150"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZN36953.1"
                     /translation="MTAGPDFTASGGLLTAVAQDAASGEVLMVAWMNAEAYAETLRTG
                     QAVYFSRSRGRLWRKGEESGHVQTVKAIYVDCDADAILLKVEQTGVACHEGYRSCFFR
                     QVTPDGLTVVAKRLVDPAAVYPRK"
     gene            5993..6592
                     /locus_tag="VFB80_24155"
     CDS             5993..6592
                     /locus_tag="VFB80_24155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN36954.1"
                     /translation="MPDSLSVIVPVRNAETSLVGQIERLLDLLPDLTPDFEILVVDDG
                     STDHTVDLARELTQRFPQVRMIRHTQPRGAEATAQTGLSWARGQTVIVQEEPAALGAT
                     ELRRLWSLRREPPAVQPRGARRADLFAPALLERLATWAQSLRHLADGGSLGSVRMIRD
                     RAADVCLGASETEHRADGAHDRPPHEARTTRATPIGKPQ"
     gene            complement(6594..7469)
                     /locus_tag="VFB80_24160"
     CDS             complement(6594..7469)
                     /locus_tag="VFB80_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN36955.1"
                     /translation="MNAAALADELSTAELHRIREVLADSLGQSPASGGEPLDRVEILE
                     RLLGPELCRKLGVFRLPAGFKLSVVMPVYNEIRTLAQVIERVRSTKLPIELVIVDDGS
                     RDGSRELLANLQHGGSDDLKVILHEKNQGKGGALKSGFLACTGDVVVIQDADLEYDPQ
                     DYRALLQPIVLGDADVVYGSRFSHVDGPVHHYWHRWGNQLITRLSNWRSGRAWTDVET
                     CYKMVRRELIQHIAPGLKERGFGVELELTAKFARLSGVRFYERPISYVGRSWAEGKKI
                     GVKDGLWALWCILRY"
     gene            7949..9274
                     /gene="panC"
                     /locus_tag="VFB80_24165"
     CDS             7949..9274
                     /gene="panC"
                     /locus_tag="VFB80_24165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZN36956.1"
                     /translation="MASCLIGLGSNLGDRVGNLWQALEELRRTPGIAVSRTSSLIETS
                     PVGGPADQPNFFNAAAVLDTELSPFEVLNLLLGVERKLGRVRSERNGPRTIDLDLLLY
                     DQEVLEAPELTLPHPRLHERRFALAPAAEIAPDWRHPTSGRTVAALLAALPFGEGGRL
                     GLRVLTSPYDTQAQVLQIRRGGQRVALVPTMGALHDGHLSLVRIARQRAEVVVASIFV
                     NPTQFGPQEDFSRYPRTLDADLSALSVAGCDLVFLPAADDIYPPGFSTSVEPPAVAQP
                     LEGVCRPGHFRGVATIVLKLFHLIPAHLACFGQKDYQQLQVIRRMTADLNLPVEIVAC
                     PTVREPDGLAMSSRNRYLSPAERQQALSISRALASAAQQVKDGERNAAAIKQQMRNTL
                     AAAGIEKTDYATVADAETLAESNRIEGPAVALIAAFVGTTRLIDNRLLP"
     gene            9340..10662
                     /locus_tag="VFB80_24170"
     CDS             9340..10662
                     /locus_tag="VFB80_24170"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZN36957.1"
                     /translation="MRSTLFYIPAELLGVPVFGVGWVLAAWVVFSLALLSWLWSRPSG
                     NREAMGYLPFVAIVAAVIVFLLPNLVVRNAAGQALGVPIRGFGVMLMLATVSAVGLAA
                     YRGWQAGIDPEEIYSLAFVMFIAGIVGARLFFVVQKWDEFAVFDANDALQLAPTLRKV
                     FNVTEGGLVVYGSVLAGLPAGIWYCLRRGLPLLVVADVIAPSMVVGQALGRIGCFLNG
                     CCYGGVCLTSSLAVTFPAESPPYREQEVQGWHSGVWLEEKNGAIVVAYVAPNCPAKQR
                     GLKVGDELDAINTVAVSDLAQARAALAMKTELPNKHFSYAVRKKGGEVIWWEVPQAPV
                     RSVPVHPTQLYSAIDAGLLALVLWLAWPFRRRDGEIFALLITLHPISRILLEVIRSDE
                     QGFWGTALTISQWLSLGILAAAGVLWWYVETNSKNQRTKEPKAEGAVT"
     gene            10709..11563
                     /locus_tag="VFB80_24175"
     CDS             10709..11563
                     /locus_tag="VFB80_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HZN36958.1"
                     /translation="MQIVITAVGPDNVRLADPIIHYVTGQGANIGEIQMYDHDEEALF
                     AMLLRVELPAEKLGEIRPALAAIGKEKKLSIRVWSAEERADRPRLAILVTLRPETPLA
                     ILRAIRDGQIRAEPAVMIGNRTNCRGIAEQFGVPWEMIGDAEGRADDDLLTQVLDQYD
                     VDYVVLARYMRILPAASCWKYAGGRIINLHHGLLPSFPGARPYHDAYAGRMLTYGATC
                     HFIVPELDAGNQTIYQSTFTVPPGMKLDEIIRLGEQDNEPRCLVEGVRRVVAREVQLH
                     FHRVVARK"
     gene            complement(11587..11847)
                     /locus_tag="VFB80_24180"
     CDS             complement(11587..11847)
                     /locus_tag="VFB80_24180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36959.1"
                     /translation="MTHRVFWSPHAERRLEQLLSNTAERPRLAAVAREIDRLLVADPL
                     AFGESRYETVRVGFVLPLGVQFEVLDDVRTVIVYDVWRVDRK"
     gene            12686..13006
                     /locus_tag="VFB80_24185"
     CDS             12686..13006
                     /locus_tag="VFB80_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HZN36960.1"
                     /translation="MPLYVYEVITDDDSPGEQFEIVQSMSDPPLKKHPQTGQPVRRVF
                     FAPAVGGKWSDSAMAKSVSDDKKLERLGFTKYVKSGDGSYEKTVGKGPDTISRDQPVS
                     PGDL"
     gene            13225..13521
                     /locus_tag="VFB80_24190"
     CDS             13225..13521
                     /locus_tag="VFB80_24190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36961.1"
                     /translation="MRRLLLAASLTLPVLSSGCALCCAPFDCHYPYVGGAWVRTDPVN
                     GRVGSAFYNAGAPAGAVPVAATEPTPAQPGAMPTPAAPGARSVIPRNMGEGYLP"
     gene            13592..14893
                     /locus_tag="VFB80_24195"
     CDS             13592..14893
                     /locus_tag="VFB80_24195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZN36962.1"
                     /translation="MPRFVTHLEGAIDGERLPADRLATVHKDRPIWVRYDLAAVGRAL
                     TKEMLAKREQTLWRYRELLPCLDAVWQRATPDLQQMWRYRHLVPHLDIDCVSLGERMT
                     PLLHCLQLGNKFGLKDLWIKDESQLPTGTFKSRGMALAITMAQRFGVRRVALPTAGNA
                     GGAAAAYAARAGLECFVFMPEDTPVVNQYECRLAGAKTFLVNGLINDCGRIVREGKER
                     LGWFDLSTLKEPYRLEGKKTMGLELAEQFDWQLPDVILYPTGGGTGLIGMWKAFAELT
                     ELGWLTGDKLPRMVSVQSDGCAPIVRAFEAGERFAAPFENARTVASGLRVPGAVGDFM
                     ILDAIRASGGTAIAVEESRLAEWQRLATSLEGIPVCPESAACVGALEALLARGWIQPH
                     ERVVIFNTGAAQKYVEAIRSELPRIDLAQPLDWERISATAQ"
     gene            15102..>15999
                     /locus_tag="VFB80_24200"
     CDS             15102..>15999
                     /locus_tag="VFB80_24200"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN36963.1"
                     /translation="MLVVIAIIATLVSLLLPAVQAAREAARRAQCQNHLKQVSLAFQN
                     FELSRRELPLAFTNQSLPGKNNWAPFVLPYLEQKNLIDGYDLAIDWWVAPNRPLVATQ
                     LKVLQCPSSPFQNRMQDKPETTPPNKTGACGDFFTPAGVHPDINLVLPAADQIGAGND
                     LRGVICWYSATNNVNRFADVADGTSNSILLGECAGREDVWRRRQRTMVNFTGSPKVRA
                     RGGAWATTDNAYEVGQRVAWDAAFGPIPGPVAINNSNEWGHCFYGFHASGANFAFADG
                     SVRVLSEGTNLRVLANLVTRAGG"
BASE COUNT         2731 a         5202 c         5138 g         2928 t
ORIGIN      
        1 cccgcaaaga cctgccggag aatctgccgc tgctctcgca tatcgcggac tttgtttccc
       61 agccaggcga ggcttacgcc gcacgccaag gtcatgatca gcagcgttcg caagccaaac
      121 cggaccggaa ttcttcgccc ggcgcgtagc agcgagggcc acgccagcct caatccatag
      181 ccgatcacca gcgcagcagc gatgcccagc agcagcagtc ccgttgctcc tacctcggtg
      241 acgccatacg taagcgcaat cagcaccagc agcagcgcgg cggcaatgaa cagcagggca
      301 atcgtccggg taatcaacga cacgagttgg cgcgcacccg gctgcatcaa agcggtctcc
      361 gctgtgccgt cgctgctgcc gagcgctcac ccctcgaact tccgccggct cgtcttctcc
      421 ttcgtcggga agtcgctcgg aatcggcagc gtgactttac cggtggcggc ccattcggcg
      481 ccgcggacga gggtagtgat gaagccgacg cactcttgcg agtcgttgcc gtggcccatg
      541 ggggtgtgaa agacgcggcc cttgccgtag tcgacggtga agatcatcgg ctcgtgccga
      601 cctgtgccgc ctttggccgg gtcggcatag gccgtggcca ggatgtgcat gttcgcgccg
      661 gggccgcgga gcttgtcgta gagctcgtcg ttgacgtgca gccactcgcg aggcatcccc
      721 ttggtcaccg ggtgctcggc gtcgcgaacg atcaggccaa acgcgtgctg cgccccgtgg
      781 tggccgccac cgccgcgttc ttcgctcttg accagcttgc cctccgcgtc gagatagata
      841 tacggcccac tggtttcatt tcgcccgtcc cagccgccca ggccgattat ctcgttgtat
      901 tccttccact tgccgaaggc attgttcgcg gcgtgcacga tcacgaggcc gccgccgccc
      961 ttcacatagt cgatgaaggc cgcctgcgtc tccttcggcc aatccgcccc gttgtagttc
     1021 gagaccacta cggcgtactt cttgaactcc ggcttgaagc tcgcgtcggt cccccgcggc
     1081 gcggtcgtgg cgacatcgac cgtgaatttg cccgtctcct ccaggtactt cttcatcatc
     1141 ttggtcgtct gcggccaatt gccgtggttg ttctggccat cgacgatgag ggctttcagc
     1201 ttgccgctct cctgggcgca cgcagcggcg ggaagcagga gagcaacagc ggcagcagcg
     1261 atgaggatgc ggtagatcat ggaccaactc ccaaaaaggc agaatcgatt tgcggcattg
     1321 tagcgtggcg ccatggggaa ttcagaactc agaacgcaga attcagaatg gtgaggcggc
     1381 ggccgccagt ttcggtggcg acttcgccga cgatgcaggc cgcttcgtcg ccgccggcgt
     1441 gcagaccccg gcacaactcg ccagcctgct cgcgggccac gccgatcagc agcccgccac
     1501 aggtttgcgg atcgaacagg gccgcgtatc cggccgaact gcggttgcac ttcgcagtgt
     1561 ccatttcacg ctccgccgcc gcgttggcgg gtgcgagcgt gctctgcatg ccgccagata
     1621 gcaattcggc agcgccgggc aacagcggca cggaatcgag ccgcagtgtt gcggcgacct
     1681 tgctcgctcg cagcatctcc agcaaatgcc cggcgaggcc gaatccggtg acatccgtca
     1741 ccgcccgcac gtcgaattcc gcgagcaact ctgccgccgg ctgattgctg cggagcatcg
     1801 cattcagcag cggctgccag cagcccgcct tgagcagtgc ctgcatatgg gccgcgagca
     1861 gcacgcctgt tcccagcggc ttggtgagga tcagcgcatc cccggcgcgc aggttccctt
     1921 tggctcgcag cggcctggct tgctccgcca ggatcgtgta gccgatcgtg agctgcggtc
     1981 cttcgatcgt atggccgccg gcaagcgttg cgcccatggc cttcagctct cgtacgccgc
     2041 cggcgagcag ttcgaacagc agttgctcct gctgctctgc cgggccttcg ggaatcgtcg
     2101 cgatcgccaa ggccgcgacc ggccgcgcgc ctttggccag aacgtcgctg gcggcgtgca
     2161 gcgcggccat ccgcccgacg agatagggat cgtccagcgg cgcggcgaag aagtcggtcg
     2221 tcgcggccag cggctggcca tcctggactc gcacgagtgc tacgtcgtcg ggctgagaca
     2281 ggcccacgag caggctgtcg tgcagcggga tttccagacg agatagcgcg cgggacagca
     2341 cggtgccgcc gattttcccg ccacagccgg cgcagcgcat ttgcggggga tccatctcgg
     2401 gccggccaga gccttgcccc atgagcatgg gcgtgtagtc ctggtacatc gccatgaact
     2461 tgcggtcgat gcggtccttc aatcgccagg cccagcggcc ggcgaacgtg aagccgcggt
     2521 actcgccaat cgcgcggccg tcgccggtgt tgatgagctt gaggaacccg cgctgcgggc
     2581 ggtacgtgac gaccggctgg ccgcggaggg cgcgctgaat gttttcccac aggatcggcc
     2641 cctcgcgcac cgcatagacg cccgccttgg ggagctcttg gccaacgatc gtgccgctgt
     2701 cgccgacggc gaagacagcc ggatcggccg tggattggag tgctggcgtg gtgagcaaga
     2761 agccgtcgtc gtccgtcggc aggccgactc ggcccagcag cggcggcgct gccgccgacg
     2821 tggcccacag aatgagatcg gccgccagcg attggccgtt gtcgagcgtg acggtgctgg
     2881 cctcgatcga cgtcacgcgg cggtccagta tcacgtttac gccgcgcgat tcgagctctc
     2941 gctggacgcg ctgggccgtc gcgtccgtcg tgccgggagt gagccgcgga tggctgttga
     3001 ccagcgtgat ttcgaagggc ctttcggcca gcagcgtccg caggcggctg gacaggcaga
     3061 atgcaatttc cacccccccg actccgccgc caacgatcac cgcccggtag atagcgccat
     3121 cgggaggccg acgcctcccg ctcgccagga cgcgactctc cagccgcgca acaaacgtct
     3181 gcatcggctt gatgggcagc agcgtattgt ccgctacttg caggccgtcg cgcgagggaa
     3241 ccgagccaat cccgaccgaa agcacgtcgt agcgcagcgg cgggcgatcg tcgaacagca
     3301 attcgcggtg ctggcgatcg aggccggtga cgttgcttag aatcagccgt gcgcccgcgg
     3361 cggcgcagag ccgcacgagg tcgatttcca tcgcctctgg tggatactgc ccggcgagca
     3421 cgcccggcag catgccggaa tacgtcgcgg tcgggaaatt cgacacgcag gtcagccggg
     3481 catcaggaat cggctgcatt cgccacatcc gcaggacgtg ggcgttggtg tggccgatgc
     3541 cgagcaggac gacatcgtgc gtgggcaagg tcggcggcat cattacgcca gcagccgctg
     3601 caccgcctcg accagcttgt ccatcgcgaa gggcttgcgc agatagtcat caacgccgag
     3661 catttcggcg taggccttgt gccgcgagcc ctcgttggcg gtgatcatga tgacgcggac
     3721 gggcacgggc cgcgtgcggc gaagtttttc gaggaccagg aatccgctcc gcttgggcat
     3781 catcatgtcg aggataacca gatcgggatc ttcgctctcg gccatcgcca gcccctggtt
     3841 gccgtcgcgg gcgatgaaga tcttgaagcc cttcgattcc agcgcgtagc ggatggcgtc
     3901 gatgatctcc gggtcgtcat cgaccaggag tactcgtttg ccagtcttgg ccggctcgtc
     3961 cttggccttt tccttgtcct tcgccatcgc agctcggttt ctcgcgtggg gcagcgtctt
     4021 ttgaccgcac agccgatagc ttataaaagc ccgtggagac aagcaatccg ctgggcaacc
     4081 ggccgcgaat cgcgaaccac catgacgagc cgctttaccg ccgacgaact ggcccaatac
     4141 ggccgcgatg gctactacat cgtacgcggg atatacgatc gcggcgaaat cgacaagctg
     4201 ctccgctttg ccaaagacga tcccagcttc gcgggcagcg tctatggccg caaggacgcc
     4261 gcaggccacg aaaccaagct ggctctctgg aatttcgcgg gcgacgacct gtacagcatg
     4321 ttcgcccgct cgccgcggat cgtagatcgc atggagcagg ccctcggggg cgaggtctat
     4381 ctctaccaca tgaagatgat gcttaaggag ccgcgggtcg gcggcgcttg ggaatggcac
     4441 caggactacg gctactggta ccacaacggc tgcctgttgc cgctgctggc cagctgcctg
     4501 atcgccgtta gccgggcgaa ccaggccaac ggctgtttgc aggtgctgcg aggctcgcac
     4561 ctgatgggcc gcatcgacca tggcaagacg ggcgaccaga cgggggcgga catggagcga
     4621 gtcaacgtcg ccctggagcg gatgccgctc gattacgtgg agtgcgagcc gggcgatgcg
     4681 atcttcttcg acagcaacct gctgcaccgc agcgacatga acggctccga cgagccgcgg
     4741 tggtcgctga tctgttgcta caacgcgcgg cggaacgacc cctacaagga ctcgcggcac
     4801 ccgcggtaca caccgctgcc aaaagtcgcg gacgacgccg tgcggcggtg ggagccgaaa
     4861 taggagaacc tatgccagct cgaagatcac ctcgacctcg acggtggcat tccgcggcag
     4921 tgcgcccatg ccgacggcgc tgcgggcgcc gataccgtga tcctggcccc agagtttggc
     4981 gaacaggtcg ctgcagccgt tgatcacttc cggatggttg gtgaagtcgg cggttgaatt
     5041 gaccataccg agcgtcttga tcacgcgttt caccttgtcg aggctgccca ggttggcccg
     5101 cagggtcgcc aggatcgcca gccccgccgc ctcggccgcc ttcttgccgc ccgcctcatc
     5161 gacctccgca ccaacgcggc cggtcaagat cttgccctgg gggccctggg ggtcgagcga
     5221 gacgtgcccc gagacgtaag ccaggttgcc gacggtgacg accggcttat agatgccgcc
     5281 cggcttggga agcggcggca gttggagctt gagttcgacg agtttggctt ccgcgctcat
     5341 gggagggctc ccttcggtgg gcaatggact gaaccgtcgg tgcgaatata cagccggcaa
     5401 tgcgagtgcg aaacccgccg cctatttccg cggatagaca gccgccggat cgactagccg
     5461 cttggcgacg acggtcagcc cgtccggcgt gacttgccgg aagaagcacg agcggtagcc
     5521 ctcatggcac gcgacgccgg tctgctcgac cttcagcagg atggcgtcgg cgtcgcaatc
     5581 gacgtagatg gctttcaccg tctgcacgtg gccgctctct tcgcccttcc gccagagcct
     5641 gccgcggctg cggctgaaat agacggcctg gccggtgcgg agcgtctcgg cgtaggcctc
     5701 ggcgttcatc caggcgacca tcagcacttc gcccgacgcg gcatcctggg cgacggccgt
     5761 gagcagtccg ccactagcgg tgaaatcggg gcctgcggtc atggggcggt ccttggtaag
     5821 gttcaaacgt tcattctacc cgccgttttc ccggtgcggc tgtcactccc cacacaatcg
     5881 gctcgccgcg aattgcccgc agggtgcgac ttccccttcc caaattgccg cgggcccacc
     5941 atgtgcgccg ataacgaccg acagccgctg ctagcatctc gaggccgcac ccgtgcccga
     6001 ttcgttgagc gttatcgttc ccgttcgcaa tgccgagacc tcgctggtcg gccaaatcga
     6061 gcgcttgctc gacctgctgc ccgatctgac gcccgacttc gagatcctcg tggtcgatga
     6121 cggatcgacc gaccacaccg tcgatctggc ccgcgagctg acccagcggt ttccccaagt
     6181 gcggatgatc cggcacaccc agccgcgcgg ggccgaagcg acggcacaga ccggcctatc
     6241 gtgggcccgc gggcagaccg tcatcgtgca ggaagaaccc gccgccctgg gtgcgaccga
     6301 gctccgccgg ctttggtcgc tgcggcgtga gcctcccgcc gtgcagccgc ggggcgcacg
     6361 ccgggcggac ctcttcgccc cggcgctact ggagcggctt gcaacctggg cgcagtcgct
     6421 gcgtcatctg gccgacggcg gttcgctggg gagcgtgcga atgatccgcg atcgtgcggc
     6481 ggacgtgtgc ttgggcgcca gtgaaacgga acatcgcgcc gacggtgctc acgatcgtcc
     6541 gccgcacgaa gcccgcacga cccgcgcgac accgatcggg aaaccgcagt agcctaatac
     6601 cgcagaatgc accacagggc ccacaggccg tctttcacgc cgatcttctt cccttcggcc
     6661 caggatcggc cgacgtagct gatcggccgc tcgtagaacc gcacgccgct caggcgggcg
     6721 aacttggctg tcagctccag ctcgacgccg aagccgcgct ctttcagccc cggcgcgatg
     6781 tgctgaatca actcccggcg aaccatcttg tagcaggtct cgacgtcggt ccaagcccgc
     6841 cccgaccgcc agttcgacag ccgcgtgatg agctggttgc cccagcggtg ccaatagtgg
     6901 tgcaccgggc cgtccacgtg gctgaagcgg ctgccgtaaa ccacgtccgc atcacccagc
     6961 acgatcggct gcaatagcgc ccggtagtct tgcgggtcgt attccaggtc ggcgtcctga
     7021 atgactacca cgtcccccgt gcaggcgagg aagccagact tcaaagctcc gcccttgccc
     7081 tggtttttct cgtgcaggat gactttcaag tcatcgctgc cgccgtgttg cagattcgcc
     7141 aggagctccc gcgagccgtc acggctgccg tcatcgacga tcaccagctc gatcggcagc
     7201 ttcgtcgagc ggacgcgctc gattacctgc gccagcgtgc gaatctcgtt gtaaacgggc
     7261 atcacgaccg agagcttgaa gccggccggc aagcgaaaga cgccgagctt gcggcagagt
     7321 tccgggccca gcagccgctc cagaatctcc acgcgatcga gcggctcgcc gccgctcgcc
     7381 ggcgactggc cgagggaatc ggcgaggacc tcgcggatgc ggtgcaactc ggcggtggaa
     7441 agctcgtcgg ccaaggcagc ggcgttcatg gtgctggtgc gtcctgattc agtttaccta
     7501 gctggtggcc gcgcgccgcg gaactgcgtg tgaagatagg gaatttcggg tccgcgactc
     7561 aatagaaaaa agcgcgagct agcgtgttga caactggccg cgacttcgca caatacgccg
     7621 agtcgtttgg ggtggtgcga tcgccaacac ggaaggcagt gatgagagca gcggctcgtg
     7681 tgacggctgg atgctcgatt cggcctccgc ttagcgacgc aatcgttcgt ggctttggga
     7741 ttgattcggc tgcgcgactg ttctggttcg cgttcgttgt tcctttctcc aacaactgtc
     7801 tggaagtgcg cgcgatgcgc gattgaatga cgtactctgg gggccgacaa ccgagcggat
     7861 cgactgtcgg cgacctggtc gtggaacgga tgccccccca tttttatctc gagccgaacg
     7921 attctggcct cctcgctata attcacacat ggcgagttgt ctcatcggtc tgggttccaa
     7981 tttgggggat cgcgttggaa atctgtggca agcgctggag gagctgcgcc gaactccagg
     8041 aattgccgtc agccgcacga gttccttgat cgagacgtca ccggtcggcg gaccggctga
     8101 tcagcccaac ttcttcaacg cggcggccgt cctggacacg gaactgtcgc ccttcgaggt
     8161 gctgaacctc ctgctgggcg tcgaacgcaa gctcggccgc gtgcgcagcg agcgtaacgg
     8221 cccgcgaacc atcgatctcg atttgctgct ctacgaccag gaagtgctgg aagcccccga
     8281 attgacgttg cctcatccgc ggctgcacga gcggcgattt gcactcgctc cggcggcgga
     8341 gatcgctccc gattggcgac atccgaccag cggccggacc gtcgcggccc tgttggcggc
     8401 cctgcccttt ggcgaaggcg gccggctcgg actgcgcgta ctgacttcac cctacgatac
     8461 gcaggcgcaa gtgcttcaaa tccgccgcgg tgggcagcgc gtcgcgctcg tgccaacgat
     8521 gggcgcgctg cacgacggac atctcagcct ggtgcgaatc gcccgccagc gcgccgaggt
     8581 cgttgtcgct tcgatcttcg tcaacccgac gcaatttggg ccccaggaag atttttcccg
     8641 gtatccgcgg accctcgatg cggacctgtc ggccctctcc gtcgccggct gcgacctggt
     8701 ctttctaccg gcggccgatg acatctatcc gcccggtttt tctacgtccg tcgagcctcc
     8761 tgcagtcgcg cagccgctgg agggcgtttg ccgtccggga cattttcgag gggtcgccac
     8821 gattgtcctc aagctgtttc acctgattcc cgcccatctg gcatgtttcg gccagaaaga
     8881 ctatcagcag ctgcaggtca tccgccgcat gaccgccgat ctcaacctgc ccgttgagat
     8941 tgtcgcctgc ccgacggtgc gcgagccgga tggtctggcg atgagcagcc gcaatcgtta
     9001 tctttcgccc gccgagaggc agcaggcgct cagcatttcg cgggccctgg caagtgctgc
     9061 ccagcaggtc aaggacggcg agcggaatgc cgcggcgatc aagcagcaga tgcggaatac
     9121 gctcgcggca gccgggatcg aaaagaccga ctatgccacc gtcgccgacg ccgaaacgct
     9181 ggcagagtcc aaccgcatcg aaggtccggc cgtcgccctg atcgccgcct tcgtcggcac
     9241 gacccgcttg atcgacaatc ggctgttacc ctgacaaagc cgcgaccttt ggtcgcgctc
     9301 tacctgaatc gcgcgaccac tggtcgcggc tttgagactg tgcgatcgac cctgttctac
     9361 atcccggcgg aattgctcgg tgtgccggtc ttcggcgtcg gttgggtgct ggccgcgtgg
     9421 gtcgtcttca gccttgcgct cctaagctgg ctttggtcac ggcccagtgg caaccgggaa
     9481 gccatgggct acttgccctt cgtggccatc gtcgcggcgg tgatcgtttt cttgctgcca
     9541 aatctggtgg tacgcaatgc ggcagggcaa gcgctcggcg tgccgatccg cggcttcggc
     9601 gtgatgctca tgctggcgac ggtcagtgct gttggcctgg cggcttaccg cggctggcag
     9661 gcgggcatcg acccggaaga gatctattcg ctggccttcg tcatgttcat cgccgggatc
     9721 gtcggggcca ggttgttctt cgtcgtacag aagtgggacg agttcgccgt gtttgacgcc
     9781 aacgacgcgc tccaactcgc cccgaccctg cgcaaggtct tcaatgtgac cgagggcgga
     9841 ctcgtcgtgt acggctcggt gctggcgggg ctgcccgcgg gaatttggta ttgcctgcgg
     9901 cgcggattgc cgctgctggt tgtcgccgac gtcatcgctc ccagcatggt ggtcggccag
     9961 gcgctcggcc gcattggctg cttcctcaac ggctgctgct atggcggcgt ctgcctgacg
    10021 agcagcctgg ccgtaacttt tcccgccgag agcccgccct atcgggagca ggaggtccag
    10081 ggctggcaca gcggagtctg gctcgaggag aaaaacggcg cgatcgtcgt cgcctacgtc
    10141 gccccaaatt gccccgctaa gcagcgcggc ctgaaagttg gcgatgagct cgatgccatc
    10201 aataccgtcg cggtcagtga cctggcccag gcccgcgccg cgctggcgat gaagacggaa
    10261 ctgccaaaca agcacttctc ttacgccgtg cggaagaagg gcggagaagt catctggtgg
    10321 gaggtcccgc aggcacccgt gcgaagcgtg cccgtgcacc cgacgcagct ttactccgcg
    10381 atcgacgccg gcctcttggc gctcgtgctc tggctggcct ggccctttcg ccgccgcgac
    10441 ggcgagattt ttgcgctgct gatcacgctg catcccatct cccgcatcct gctggaggtc
    10501 atccgcagcg acgagcaagg cttctggggc acggcgctta ccatctcgca atggctgagc
    10561 ctcgggattc tggccgcggc cggcgtgtta tggtggtatg tggagaccaa ctccaagaac
    10621 caaagaacca aagaaccaaa ggcggaagga gcggtaacat gactcagttt ggttcttcgg
    10681 ttcttcggtt cttcgatttt tgattctcat gcaaatcgtc attaccgccg tcggccccga
    10741 caacgtccgc ctggcggacc cgatcatcca ctacgtgacc ggccagggcg cgaacatcgg
    10801 cgaaatccag atgtacgacc atgacgaaga ggccctcttc gccatgctcc tgcgtgtgga
    10861 attgcccgcc gagaaactgg gggaaatccg cccggcactg gccgcgatcg gcaaggagaa
    10921 aaaactctcg attcgcgtct ggtctgcgga ggagcgggcc gaccggccgc ggctggcgat
    10981 cctggtgacg ctgcggccgg agacgccgct agccattttg cgggcgatcc gcgatggcca
    11041 gatccgcgcc gagcccgcgg tgatgatcgg caaccggacg aattgccgcg gcatcgccga
    11101 gcagttcggc gtcccctggg agatgatcgg cgacgccgaa ggccgggccg atgacgacct
    11161 cctgactcag gtgctcgacc agtacgatgt cgattacgtg gtgctggccc gctacatgcg
    11221 catcctgccg gccgcgagct gctggaaata cgccggggga aggatcatca atctgcatca
    11281 cggcctgctg ccgagctttc ccggggcgcg gccctatcac gacgcctacg ccggccggat
    11341 gctgacctac ggggcgacct gccacttcat cgtccccgag ctcgatgccg gcaaccagac
    11401 gatctatcaa tcaacgttca ccgtcccacc cggcatgaag ctcgacgaga tcatccgcct
    11461 cggcgagcag gacaacgagc ctcgctgcct cgttgaaggc gtccgccgcg tcgtcgcccg
    11521 cgaggtccag ctccacttcc accgcgtcgt ggcccggaag taggcgctgc gggccggaat
    11581 cgcaggctac tttcgatcaa ctcgccagac gtcgtagacg atcacggtcc ggacgtcgtc
    11641 gaggacttcg aattgaacgc ccagaggcag cacaaatccg acccgcaccg tttcgtatcg
    11701 tgattcaccg aatgccagcg gatcggcgac aagtagccga tcgatctccc tggcgacggc
    11761 tgccaggcgc ggtctctcgg cggtgttcga gagcagctgc tccaagcgcc gctcggcatg
    11821 cggcgaccag aacacgcggt gggtcattgg atgcccaagc gattcaggat ttcctctagc
    11881 ggcttgccgg agcggtcttt gcgcagctcg ttgatctccg cgatcgacag cgggggctcc
    11941 aaacgcaaat cgtctgcgcg tggacagccg cccaatgggg aaaagaaacc gattgctcga
    12001 ccgctggaat cgcaaagcac ggcctgccca tccagttcgg ccagcttctc gcctagcgcg
    12061 gactcgacaa ttatcttctc catatttgga tctccgctgc tctattctaa accgatcggg
    12121 cggtgcagtc actttcacgc cccgctcttc cgggtaggcg aaagttgacc gaccacaacc
    12181 tgtagtggtt cggcgaaccg caaacacaag atgttgtgtt tctcttgaca taatatgtac
    12241 ggatgataca attctccaac acccggcgat cgcatcgcgg tcggcgagtg cggctctttt
    12301 acaagttggt tgtgttacgg cagcggcggt tcggccgggt ctgcgtcgta cactcgccgc
    12361 accggattct tcgccacgcc aaaatcgccg gatgtgcggt acccgaaagg atttgcgccc
    12421 caaagcggcg aaatattcgc cagctcccca ggtgttgggc ctgtttggcg ataaattcgg
    12481 ccctctgggc ggctcaccaa tcggcgcaac tcatctaccg gcagcaactt acctaacatg
    12541 gagccaactc acgcggccgt attcttcgcc acggaggtca aaaacctggc gaacactttt
    12601 tgctccggcc agtcaaccgt tgcccaggcg ctgcgccgcg agtaaaagtg aagtggaggg
    12661 cccgtttcgg tttcgggaga tggccatgcc actttatgtg tacgaggtga ttaccgacga
    12721 cgactcgcct ggcgagcagt tcgagatcgt gcagagcatg tctgatccgc cgctcaaaaa
    12781 gcatccccag accggccagc cagtgcggcg cgtgtttttc gccccggccg tcggcggcaa
    12841 gtggtctgac tcggcgatgg ccaaatcggt ttccgatgac aaaaagctgg aacgcctggg
    12901 cttcaccaag tacgtgaagt ccggcgacgg cagctacgag aagaccgtcg gcaaaggccc
    12961 cgacaccatc agccgcgacc agccggtatc gccgggcgac ttgtaggtcc cgcctgccgg
    13021 gcgggacctg gcagcgcggt gcgttcgaat tctcgggaaa gatcctggtc gctgcgcttc
    13081 cccaaaggcc tttccggcag aaggggccta ctttctaggt tggcggcaaa ttcgctccat
    13141 ccggcaaagt ttgcataaag gtcgccgctt tccgctgccg aaaatggcgg agaacgtgcc
    13201 gtcctctttg ccgggagtgc ttgcatgcgc cgcttgctgc tcgctgccag tttgacgctg
    13261 cctgtgcttt ccagcggctg cgcgctgtgc tgtgctccct tcgactgcca ctatccctat
    13321 gtgggcggcg cgtgggtgcg gaccgatccg gtcaatgggc gagtgggctc ggcgttttat
    13381 aatgccggag cgccggctgg tgcggttccg gtggcggcaa cggagccgac tcccgcgcag
    13441 ccaggagcca tgccgacgcc ggccgcgcca ggtgcgcgaa gcgtcattcc gcggaacatg
    13501 ggcgagggct atctgccgta gtcgttgccg gcccagccag cgtggattag ggtggtcgca
    13561 gcaaatttgc cacgatcccc tgagcgccag catgccaagg tttgtcacgc atttggaagg
    13621 cgcgattgac ggcgagcgat tgcccgcgga tcgcctggct acggttcaca aggaccggcc
    13681 gatctgggtg cgatacgacc tggcggcggt tgggcgggcg ctgacgaaag agatgctcgc
    13741 caagcgagag cagacgctct ggcgttaccg cgagttgttg ccctgtcttg acgcggtctg
    13801 gcagcgtgca actcccgacc tgcagcagat gtggcgctat cggcatcttg tgccgcacct
    13861 ggacatcgac tgcgtctctc tgggcgagcg gatgaccccg ctgctccatt gcctgcagtt
    13921 gggcaacaag ttcggcctga aggacttgtg gatcaaggac gagtcgcagc tcccgacggg
    13981 caccttcaag tcccgcggca tggcgctggc gatcacgatg gcccagcgct tcggcgtcag
    14041 gcgcgtcgca ctgcccacgg caggcaacgc cggcggcgcg gcggctgctt atgcggctcg
    14101 cgctggcctg gaatgcttcg tgttcatgcc cgaggacacg ccggtcgtca accagtacga
    14161 atgccggctg gccggagcga aaacgtttct ggtcaacggc ctgatcaacg actgcggccg
    14221 catcgtccgc gaaggcaagg agcggctggg ctggttcgat ctttcgacgc tcaaagagcc
    14281 gtatcgcctg gaaggcaaga aaacgatggg gctggagttg gccgagcagt tcgactggca
    14341 actgcccgac gtgatcctgt atccgaccgg cggcggcacc ggcctcatcg gcatgtggaa
    14401 ggcgttcgcc gagctgaccg agctgggctg gttgacgggc gacaagctgc cgcggatggt
    14461 cagcgtgcag tccgacggct gcgcgccgat cgtgcgggcc tttgaagccg gcgagcgctt
    14521 tgccgcgcca tttgagaatg cccgcacggt cgccagcggc ctgcgcgtgc ccggcgctgt
    14581 cggcgacttt atgatcctgg acgccatccg cgcgagcggc ggaacggcga tcgccgtgga
    14641 ggaatctcga ctggcggagt ggcaacgatt ggcaacgtca ctggaaggca tccccgtttg
    14701 ccccgagtcg gccgcttgcg tcggcgcact tgaggcgctc ctggcccgcg gctggattca
    14761 gccgcacgag cgggtcgtga tcttcaacac gggcgcggct cagaaatacg tcgaggccat
    14821 ccgcagcgag ctgccaagga tcgatctggc gcagccgctg gattgggagc ggatttcggc
    14881 gactgcgcag tagtgcgtcg catttctcgg cgcggctgtc gagggaaacg gcagcccggc
    14941 gaatcgccta gctggcggcc cttcgcaagt cgtgtgccgc taatgacttg cgaacggtaa
    15001 acgctccggc atgcaatctg cttttgttgc cggcccacgc gatactccac gcacccggaa
    15061 ccactatgcg cttgcgacat cacggtttta ccctcgttga gttgctcgtc gtcattgcga
    15121 tcatcgcgac gctcgtgtca ctgctcctgc ccgccgtgca agctgcccga gaggcagccc
    15181 ggcgggcgca gtgccagaac cacctcaagc aggtcagcct ggcgtttcaa aacttcgagc
    15241 tcagccggcg ggagctgccg ctggccttta cgaaccagtc gctgccgggc aagaacaatt
    15301 gggccccgtt cgtcctgcct tacctcgagc agaagaacct gattgacggc tacgacctgg
    15361 cgatcgactg gtgggtcgcg ccgaaccggc cgctggtggc gacgcagctc aaggtgctgc
    15421 agtgcccttc gtcaccgttt cagaaccgaa tgcaggacaa gcccgagacg acgcccccga
    15481 acaagacggg cgcttgcggc gacttcttca cgccggcggg cgtccacccg gatatcaacc
    15541 tggttctgcc ggcggccgat cagatcggcg ccggaaacga cctgcgcggc gtgatttgct
    15601 ggtacagcgc aaccaacaac gtcaatcggt tcgcggacgt cgcggacggc acctccaact
    15661 cgattctgct gggcgagtgc gcgggccgcg aagacgtgtg gcggcggcgg cagcggacca
    15721 tggtcaactt cacgggcagt ccgaaggtgc gcgctcgcgg cggtgcttgg gccacgacgg
    15781 acaacgccta tgaggtcggc cagcgcgtgg cctgggatgc cgccttcgga ccgattcccg
    15841 gaccagtcgc gatcaacaac tccaacgaat ggggccactg cttttacggc ttccacgcga
    15901 gcggggcgaa cttcgcgttt gccgacggct cggtgcgcgt gctgtccgaa ggcacgaacc
    15961 tgcgcgtgct ggccaacctg gtgacccgcg ccggcggcg
//
LOCUS       DASRJB010000919        11298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3090467, whole genome shotgun sequence.
ACCESSION   DASRJB010000919 DASRJB010000000
VERSION     DASRJB010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090467"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..401)
                     /locus_tag="VFB80_24205"
     CDS             complement(180..401)
                     /locus_tag="VFB80_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36964.1"
                     /translation="MRYRVSWDPDAFRALMRAWIAASRPEAAIRAFDAIEATLSVDAH
                     LQGESREGERRILIVPPILDAWMIAGSSG"
     gene            complement(398..631)
                     /locus_tag="VFB80_24210"
     CDS             complement(398..631)
                     /locus_tag="VFB80_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36965.1"
                     /translation="MSTIILDTSSAEILRKCAGSAVLRDASGNVVGYFEPVPRIYQPG
                     EVPEFDSAELDRREQRWEGIPSAEARRRLESLR"
     gene            788..1645
                     /locus_tag="VFB80_24215"
     CDS             788..1645
                     /locus_tag="VFB80_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZN36966.1"
                     /translation="MKLAKYLLPSGEAGVARVEGDQLVPLDLSAGQYRSLAEVLEADN
                     PYEAAEFLSNNAEGFPLASVSLLPPIDGQEVWAAGVTYKRSRAARMEESAAAASCYDR
                     VYASPRPELFFKASPHRVAGHGQPLRIRADSNWNVPEPELTLVLSSKLQLVGFTVGND
                     MSSRDIEGDNPLYLPQAKCYNQCCGLGPWITLSVGMPPRSEIGIRLVIHRAGQAIYDG
                     RTSIEHMARTFENLIGWLGRDNSFPTGVFLLTGTGIVPESPFTLEPGDIVEIKIDGVG
                     TLLNPIVQG"
     gene            1696..3210
                     /locus_tag="VFB80_24220"
     CDS             1696..3210
                     /locus_tag="VFB80_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HZN36967.1"
                     /translation="MPHSVLVANRWRLANSSGYFRAENPATKEPLDEYPVSTWDDCDE
                     ALSAAVEAFEELRKLPAAKLAEFLEAYAGRIEARKDEIVKLASQETALPVTPRLAGNE
                     LPRTTDQLRQAAAAAREGAWAAPTIDAKNNIRSMYGPIGPVIVFGPNNFPLAFNSASG
                     GDFAAAIAAGNPVIAKANSSHPGTTRLLAEEAHAAAQATGMPAGSVQLIYRTSHEDGE
                     RLVSDPRVGATGYTGSRAAGLKLKAAADKAGKPIYLELSSVNPVVVLPGALSERMDDI
                     VGQFVASSLLGTGQFCTNPGLLILLAGEPTESFLAKVVEKFKAAPVGTLLSAGVAKSL
                     QQGVGMLIRAGAHTLVAAKGSGGQGYCQPNTLLRASGQQFLEAPDALQTEAFGNAALA
                     VVAEDLEEGCQVVEHLEGNLTGCIYSHTGGGDDAAYDRLAFRLRPKVGRLLNDKMPTG
                     VAVSPAMNHGGPYPATGHPGFTAVGIPAALRRFAVLQCYDGVRPHRLPAALRDR"
     gene            complement(3282..4049)
                     /locus_tag="VFB80_24225"
     CDS             complement(3282..4049)
                     /locus_tag="VFB80_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN36968.1"
                     /translation="MLPKNSTFIVSGGASGLGAGSVRRLVGAEANVVIADVNAAAGEA
                     LARELAPRAAFASTDVTSADSAQAAVDLAGGRFGALHGLVNCAGILGAARIVGRDGPH
                     DLGLFQKVIYVNLIGTFNMLRLAAAAMARNDPNAEGERGVIINTASVAAFEGQIGQAA
                     YAASKGGVASLTLPAARELSRFGIRVVAIAPGIFDTAMMAAAPDAVRTSLAEQAAFPP
                     RFGRPDEFALLVQQIIENPMLNGCVLRLDGAVRMAAK"
     gene            4132..5082
                     /locus_tag="VFB80_24230"
     CDS             4132..5082
                     /locus_tag="VFB80_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36969.1"
                     /translation="MRRLDLERIEIEGRPMPATPASVTSLERQLGIKLPHGYADYVTT
                     LGDGLLCGYVRVYLPDRVASELKPWRRRIKQHWFWNQTQAILQQDAALKSVVLADTME
                     GDELIYNPATGRQYVLPRHLEEAFEVGTDFLAAMDWFCSSGVLLPKTKDCRFEPATSE
                     RQKTEVIERAPATRKSTHREKQHREGRRLLIMSASHSASGTVTTSAYFLTPQHRRDGW
                     DGTSKFAFDMEAIISSAQAAATRGAKQPPVVTSIRVARTFVGKDIAAYYYVTVEFTTN
                     KGASSQVLLDLDGNVVEPEVKTFRNHDKYKEYEDSLRAAY"
     gene            5086..6918
                     /gene="mnmG"
                     /locus_tag="VFB80_24235"
     CDS             5086..6918
                     /gene="mnmG"
                     /locus_tag="VFB80_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZN36970.1"
                     /translation="MTKSPYDYDVLVIGAGHAGAEAAMAAARIGARVGLLTTNCDTVA
                     QMSCNPAIGGVAKGQIVREIDALGGVMGRAIDATGIQFRLLNRRKGPAMHSPRAQADK
                     KGYQFEVKRLVEEQPGLVLRQEVVEDLLFEERVQDSGFRVLGVRVRGGVEYRAAAVVL
                     TTGTFLSAIMHTGEVKTPGGRAGEGTTTGISGALHRLGLRALRFKTGTPCRLNGRSID
                     YSQCELQPGDDDPEPFSFLTGEDGRQRIAENQIPCWITHTNHAVHELIRANLHRAPMY
                     SGQIQSRGPRYCPSIEDKVVRFADKESHQLFLEPEGRQTHEVYVNGISTSLPRDVQDA
                     MLRLIPGLQNAQIMRYGYAVEYDYCPPDQLWPSLETKLVSGLYFAGQINGTTGYEEAA
                     AQGLVAGANAALAQRGQEPLAIGREDAYIGVLIDDLVTCGVDEPYRMFTSRAEFRLLL
                     RHDNADRRLTSLGRSAGLVDDIRWQRLQAKEAEIAAALVLLAETRLQGVTLEQLLRRP
                     EVEWAEVMQHEPRLAAISREAALQVTYDLKYAGYVSRQEQQVARQRRLADKRIPESFN
                     YQSIRHLRTEAREKLARIRPLSLAQASRISGITPADIALVMAHL"
     gene            complement(7027..7713)
                     /locus_tag="VFB80_24240"
     CDS             complement(7027..7713)
                     /locus_tag="VFB80_24240"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZN36971.1"
                     /translation="MRLAFTSLVMLMFARSLLAADAAPNTLTDEEKAAGWKLLFDGKT
                     TSGWRNYNKTTIAPGWKVENGALVRGSEAGGDIVTEGKYGAFELSLEYNISKGGNSGL
                     MFHVEETSNPPWHTGPEIQIQDVGGRDPQKPGWLYQIYQAEVDAAKPAGEWNQLKILI
                     TPEKCATWMNGKLYYEYVKGSKDWDERVAKSKFAKMPKFGKPTSGVICLQDHGNPVSF
                     RNIKIREVGK"
     gene            8865..10121
                     /gene="eno"
                     /locus_tag="VFB80_24245"
     CDS             8865..10121
                     /gene="eno"
                     /locus_tag="VFB80_24245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZN36972.1"
                     /translation="MAKITRIHAREILDSRGKPTVEAEVHLESGVIGRASVPSGASTG
                     AAEARELRDADAARYDGLGVRRAVDHVRQRIAPALIGADAGDQAEIDRRMLGLDGSPN
                     KANLGANAILAVSLAAARAAALAKGLPLYRHLADLAKCSPRMPLPMVNMISGGKHAGG
                     NLDFQDVLIIPAGASDFPTQLEWIVRIYRRLGRLLNEAGYEGHLVGDEGGYGPRLKSN
                     REAVEFVIRAIEAAGLRPRADVTLALDVAASHFYQSGGYCLAAEQGQRLTSGQLIDRL
                     EQWAAEFPIASIEDGLAENDWPGWQELTARLGSRVKLVGDDLFATNERLLKRGIDERV
                     ANAILIKVNQIGTLSETFATLRRARQHGYACIVSARSGETEDDFLADLAVGTAADYIK
                     VGSVARSERLAKYNRLLRIAEELQDS"
     gene            complement(10129..11094)
                     /locus_tag="VFB80_24250"
     CDS             complement(10129..11094)
                     /locus_tag="VFB80_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZN36973.1"
                     /translation="MLRSLYTPIAAELAEVEALLRSELRSDYPFVDELVRYGCLLGGK
                     RLRPALLLLTAKAAGERVTRDQITLAAVVEMIHTATLVHDDVLDEAQIRRHLATVNAR
                     WDNEASVLLGDFLFTHAFYLASTLDSVQGCRLIGRATNIVCEGELRQKGSRGNFLLGE
                     AEYLEIIEAKTAELTAVSCRLGALFAGAAEKVAIELDGFGRDLGIAFQIADDLLDVLG
                     EEQTTGKSLGTDLEKQKPTLPIIRALELATTADRRAILDLVGGGDCRADSLAPYLERY
                     DAVEYARQQAQSFADRARRRLDVLRPSPARQVLSAMAEFVVSRSM"
BASE COUNT         2017 a         3820 c         3610 g         1851 t
ORIGIN      
        1 gatccaggat ctcgagattc ctacaatcaa caagctcttc atccacacgc agcccgccca
       61 cctgcaaaag ctgcgcggcg ccgagctgcc ttcggccgac cgcaacgtcg agcgggccat
      121 gtatctgcag gcccacctcc gccccaacag caacggcggc tcggcgaagg ataactaagt
      181 tagccgctgc tgcccgcgat catccaggcg tcaagaatcg gcggcacaat aaggatccgc
      241 cgctcgcctt cgcgcgattc gccttggagg tgggcgtcaa cactgagggt ggcctcgatg
      301 gcgtcgaacg cgcgaatcgc ggcctcaggt cggctggcgg cgatccacgc tcgcatcaag
      361 gcgcggaatg cgtccggatc ccacgagacg cggtatctca ccgcagcgac tccagccggc
      421 ggcgggcttc ggcggagggg atgccttccc agcgctgttc gcggcggtcg agctccgctg
      481 agtcaaactc gggaacctcg cccggctgat aaattcgcgg gacaggttca aagtagccga
      541 caacattgcc gctggcgtcg cgcagcacag cggacccagc acatttccgc aagatctccg
      601 ccgaggaagt atccagaatg atcgtgctca tcgtccacct cggtcagcgt cacgcactac
      661 ccagataatt ctagtccacg aacaagaact ctcccagttg cacttgccaa gattgggacg
      721 gggccagctt cgcaattggc ctgctacaat ggcgatccgg tgcaattctc ctggaagaac
      781 cagctccatg aagctcgcca aatacttact gcctagtggc gaggccggcg tcgcgcgggt
      841 ggagggggac cagctcgtgc cgctcgacct ctccgccggc cagtaccgct cgctcgccga
      901 ggtgctggag gccgacaatc cgtacgaagc cgccgagttc ctcagcaaca acgcagaggg
      961 gttccccctg gccagcgtca gcctgctgcc gcccatcgac gggcaggagg tttgggccgc
     1021 cggcgtcacc tacaagcgga gccgggctgc ccgcatggaa gaatcggcgg ctgccgcgtc
     1081 gtgctacgac cgcgtctatg cctcgccgcg gccggagcta ttcttcaagg cctcgccgca
     1141 ccgcgttgcc ggccacggcc agccgttgcg gattcgcgcc gattcgaact ggaacgtccc
     1201 ggagccggag ctgacgctcg tgctgtccag caagctgcaa ctcgtgggct ttacggtcgg
     1261 caacgacatg agcagtcgcg acatcgaagg cgacaacccg ctctatctgc cgcaggccaa
     1321 gtgctacaac cagtgctgcg gcctcgggcc gtggatcacg ctcagcgtcg gcatgccgcc
     1381 gcgcagcgaa atcggcattc gcctggtgat tcaccgcgcc ggccaggcga tctacgacgg
     1441 ccgcaccagc atcgagcaca tggcccgcac gttcgagaac ctgatcggct ggctcggccg
     1501 cgataacagc tttcccaccg gcgttttcct gctcaccggc accggcatcg tccccgaatc
     1561 gccttttacg ctcgagcccg gcgacatcgt cgagatcaag atcgatggcg tcggcacgct
     1621 gctgaacccg atcgtgcagg ggtaattcat ccgccgccct accgattaac gaccaccgac
     1681 ttccgaccaa cccgcatgcc ccactctgtc ctcgtcgcca atcgctggcg cctcgccaac
     1741 tcctccggct acttccgcgc cgagaatccc gcgacgaagg agccgctcga cgaatatcca
     1801 gtgagcactt gggacgactg cgacgaggcg ctttcggcag cggtggaagc attcgaagaa
     1861 ctccgcaagc tgccggcggc gaagctggcg gagttcctcg aggcgtacgc gggccggatc
     1921 gaggcccgca aggacgaaat cgtcaagctg gccagtcaag agacggcgct gcccgtgacg
     1981 ccgcgcttgg ccggcaatga gctgccgcga acgacggacc agctgcggca ggcggcggcc
     2041 gcggctcgcg aaggcgcgtg ggccgcgccg acgatcgacg ccaagaacaa catccgctcg
     2101 atgtacggcc cgatcggccc ggtcatcgtc ttcgggccca acaactttcc actggccttc
     2161 aatagcgcca gcggcggcga ctttgcggct gcgatcgcag ccggcaaccc agtcatcgcc
     2221 aaagccaaca gctcccatcc cggaacgacg cggctcttgg ccgaagaagc ccatgcggcg
     2281 gcccaggcca ctggcatgcc ggcgggatcc gtgcagctga tctatcgcac cagccacgaa
     2341 gacggcgagc ggctggtgtc cgacccgcgc gtcggcgcga ccggctatac cggcagccgg
     2401 gcggcgggcc tcaagctgaa ggcggcggcc gacaaagcgg gcaagccgat ctacctggag
     2461 ctgtcgagtg tcaatcccgt cgtggttcta cccggcgcgc tctctgagcg catggacgac
     2521 atcgtcgggc agttcgtcgc cagcagcctg ctgggcacgg gccaattctg caccaatccg
     2581 ggcttgctca tcctgctggc tggcgagccg accgagagct ttctggccaa agtcgtcgag
     2641 aagttcaaag ccgcgccggt cgggacgctg ctgtcggccg gcgtcgccaa gtcgctgcag
     2701 caaggcgtag gaatgttgat ccgcgccggc gctcatacgc tcgtcgccgc caaggggagc
     2761 ggagggcaag gctattgcca gcccaacacg ctgctgcggg ccagcggcca gcagttcctc
     2821 gaagcgccgg acgcattgca aacggaggcc ttcggcaacg ccgcgctggc tgtcgtcgcc
     2881 gaagacctcg aggagggctg ccaagtcgtc gagcacctgg aaggcaatct cacgggctgc
     2941 atttattcgc acaccggggg cggcgacgac gcggcctacg accgcctcgc gttccgattg
     3001 cggccgaagg tcggccgact gctcaacgac aagatgccga ccggcgtcgc cgtcagcccc
     3061 gcaatgaacc acggcggccc ttacccggcc accggccacc ccgggttcac cgccgtcggc
     3121 attcccgccg ccctccgccg cttcgccgta ctccagtgct acgacggggt gcgaccgcac
     3181 cggttacccg cggcgctgcg agataggtag taggctcact ccgtgagccg tcgctcacga
     3241 tcgatgccga tttgcaaacg acggcacacg gagtgtgcct gctactttgc cgccatccga
     3301 acggcgccgt cgagccgcag gacgcagccg ttgagcatcg gattctcgat gatctgctgc
     3361 acgagcagcg caaactcatc ggggcggccg aagcgcggcg ggaatgcagc ctgctcggcg
     3421 agcgaggtgc gaaccgcatc tggcgcggcg gccatcatcg ccgtgtcgaa gatgcccggc
     3481 gcgatcgcga cgacgcgaat accgaaccgc gacagctcgc gggcggccgg cagcgtcagc
     3541 gaggcgacgc cgcccttcga cgccgcatac gccgcctggc caatctgccc ctcgaacgcc
     3601 gccaccgacg cggtgttaat gatgacgccg cgttccccct cggcattggg atcattccgg
     3661 gccattgccg ccgctgccag ccgcagcatg ttgaacgtgc cgatgaggtt tacgtagatc
     3721 accttctgaa acagccccaa gtcgtgcggc ccgtcgcggc cgacgattcg cgccgcgcca
     3781 agaatgcccg cgcagttcac caggccatgc aatgcgccga atcgcccgcc ggccaggtcc
     3841 acggcagcct gcgccgagtc ggcggaggtc acgtccgtgc tcgcaaatgc ggcccgcggc
     3901 gccagttccc gcgccaaggc ctcaccggct gccgcgttga cgtcggcgat gacgacgttc
     3961 gcctcagcgc cgaccagccg ccgtacgctc cccgcgccga gccccgaggc gccaccggag
     4021 acaatgaagg tgctgttttt cggtaacatc gtgatccgcc tttgcggcga gcggtgcgac
     4081 acctacgata agtcgtggct gtgttgaatg gcaccgtgtg gagaggcaac catgcgcaga
     4141 ctcgacctgg aacgaatcga aatagaaggc cggccgatgc ccgccacgcc ggccagcgtc
     4201 acgagcttgg aaaggcaact gggcatcaag cttccccacg gctatgccga ctacgttacg
     4261 acgctgggag acggcctgct ttgcggttat gtgcgagtgt atttgcccga tcgcgtcgct
     4321 agcgagctca aaccctggcg gcggcgcatc aagcagcatt ggttttggaa tcagacgcag
     4381 gccattctac aacaggatgc ggcgctgaag tcggttgtct tggccgacac gatggaaggc
     4441 gatgagctga tttataatcc cgcaaccggc cgccaatatg tattgccgag gcacttggaa
     4501 gaagccttcg aagtcggcac cgatttcctg gcagccatgg actggttctg cagttcggga
     4561 gttctcctcc ccaagacgaa ggactgccgc ttcgagcctg caacgagcga aaggcagaag
     4621 accgaggtca tcgagcgagc tcccgcgacc agaaaatcaa cccaccgtga aaagcaacac
     4681 cgcgaaggcc gacgcctgct gataatgtcc gccagccata gtgcttcggg caccgttacg
     4741 accagcgcct acttcttgac gccgcagcat cggcgcgatg gctgggatgg aacttcgaag
     4801 ttcgccttcg acatggaagc gatcatcagc tcggcgcaag ctgcggccac gcgcggggcg
     4861 aagcagccgc cggtggtgac ttccatccga gtggcccgca cgtttgtcgg aaaggatata
     4921 gcggcgtatt actatgtgac cgtcgaattc acgacgaaca aaggcgcttc gagtcaggtg
     4981 ctcctggatt tggatggcaa cgtcgtggaa ccggaagtca agacattccg caaccacgac
     5041 aaatacaaag agtatgagga ctcgttgcgg gcagcctatt aagtcatgac caagtcgcct
     5101 tatgactacg atgtgctcgt gatcggcgcg ggccatgccg gcgcggaggc ggccatggcc
     5161 gcggcgcgga tcggggcgcg ggtcggcctg ctgacgacga attgcgacac cgtcgcgcag
     5221 atgagctgca acccggcgat cggcggcgtg gccaaggggc agatcgttcg cgaaatcgac
     5281 gccttgggcg gcgtcatggg tcgggcgatc gacgccacgg gtatccagtt ccgcctgctc
     5341 aaccgccgca aaggccccgc catgcacagt ccgcgggccc aggccgacaa aaagggctat
     5401 cagttcgagg tcaagcggct ggtcgaggag cagccgggac tcgtgctgcg gcaggaggtg
     5461 gtggaagatt tgctgtttga ggaaagagtt caggattcgg ggttcagggt tctgggggtg
     5521 agagtccgcg gcggcgtcga atatcgggcg gcagcggtcg tgctgacgac ggggacattc
     5581 ttgtcggcga tcatgcacac gggcgaggtg aagactcccg gcgggcgggc gggggaagga
     5641 accacgacgg gcatcagcgg cgcgctgcat cggctgggcc ttcgcgccct gcggttcaag
     5701 actggcacac cctgccggct caacggacgc tcgatcgact attcgcagtg cgagctgcag
     5761 ccgggcgacg acgacccgga gccgttctcg tttctcacgg gcgaggacgg ccgccagcga
     5821 atcgccgaga accagattcc ctgctggatc acgcatacta atcacgccgt ccacgagctg
     5881 atccgggcca acctgcaccg cgccccgatg tacagcgggc agatccagtc gcgcgggccg
     5941 cggtattgcc cgtcgatcga agacaaggtg gtgcggttcg ccgacaagga gagccatcag
     6001 ctctttctgg agcccgaggg ccgccagacc cacgaggtct atgtcaacgg catctccacc
     6061 agcctgccgc gggacgtgca ggacgcgatg ctgcggctga ttccgggcct gcaaaacgcc
     6121 cagatcatgc gctacggcta cgccgtcgag tacgactatt gcccgcccga tcagctctgg
     6181 ccgtcgctgg aaacgaagct cgtcagcggc ctctatttcg ccggccagat caacggcacg
     6241 acgggctacg aggaggccgc ggcgcagggg ctggtcgccg gcgcgaacgc ggcgttggcg
     6301 cagcgtggac aggagccgct ggccatcggc cgcgaagacg cgtacatcgg cgtgttgatc
     6361 gatgacctcg tcacctgcgg cgtggatgag ccctatcgca tgttcaccag ccgggcggaa
     6421 ttccggctcc tcctgcggca cgacaacgcc gaccggcgac tgacctcgct gggccgctcg
     6481 gcgggcctgg tggacgacat ccgctggcag cggctgcaag ccaaagaggc cgaaatcgcg
     6541 gccgccctgg tgctgctggc tgagacgcgt ttgcagggcg tgacgctcga gcagctgctc
     6601 cgccggcccg aagtcgagtg ggccgaagtg atgcagcacg agccgcggct ggcggcgatc
     6661 tcgcgggagg ccgccctgca agtgacctac gacctcaaat acgccggcta cgtcagccgg
     6721 caggagcagc aggtcgcccg ccagcggcgc ctggccgaca agcggattcc cgagagcttc
     6781 aactaccagt cgatccgcca cctacggact gaggcccgcg agaagctggc ccgcatccgc
     6841 ccgctctcgc tcgcccaggc cagccgcatc agcggcatca cgccggccga catcgcgctc
     6901 gtgatggcgc acctgtagct ccctgacgcc gtcagggagc agccgagcga acgggtcgta
     6961 aatctcgggg aattcgtccc atcgggaaga ccaattgccg ctcccgttcc tcacggcgtg
     7021 aggaacctac tttccgacct cacgaatctt gatattgcgg aagctcaccg gattcccgtg
     7081 gtcctgcagg cagatcacgc cgctggtggg cttgccgaat ttcggcatct tggcgaactt
     7141 gctcttggcg acccgctcgt cccagtcctt cgagcccttc acgtattcgt agtacagctt
     7201 gccgttcatc caggtcgcgc acttctcggg cgtgatcagg atcttgagct gattccactc
     7261 gcctgccggc ttggcggcat cgacctcggc ctggtagatc tgatacagcc agccgggctt
     7321 ctgcggatcg cggccgccga cgtcttgaat ctggatttcg gggccggtgt gccagggcgg
     7381 attgctcgtc tcttcgacgt ggaacatcag gccgctgttg ccgcctttgg agatgttgta
     7441 ttccagcgac agctcgaacg ccccgtactt gccctccgtc acgatgtcgc ctcccgcctc
     7501 gctgccgcgg accagcgccc cattctcgac cttccacccc ggcgcgatcg tcgtcttgtt
     7561 gtagttccgc cagccgctcg tcgtcttccc atcaaatagc agcttccagc cggcggcctt
     7621 ctcttcgtcc gtcagcgtat tcggcgcggc atcggcggcc agcagcgagc gggcaaacat
     7681 caacatgacc agcgaggtga atgcaaggcg catggaaagt gctccctagg gaaaggatga
     7741 cggacaaatt gtagcacgcc gcgcgaacca agcgtcgatc accgccgccg ggctcgcttc
     7801 aatcggcggc gctcttgccg gctgaggatc tcgtcaactc tcaagcccag ttgctccggc
     7861 gtgatctgaa tttgcgtggc aattgttccg ttctttgcca gtgccggcgc ggcttgaaca
     7921 ggtccgaaaa accgccggcg ggcgatttgc tgactcggtt caaggccatc gcaagtcctg
     7981 tcttcggcgg cagttgcggg cgactcgtcg ctcgcattcg ccgccgcctc ggtctgcgat
     8041 actcgattct ctgaacagtc cctaaccggg tggcttgtag ggtgggctgt gcccgcctcc
     8101 gcgccgaccg cctgcgaaaa aaacgcaggc ttcttgttcg ctggtgggca cagcccaccc
     8161 tacgtgctct tcctccggcg ggcagagcaa tccgccatag gcgcctaact ccgcctgggc
     8221 cgtcgccaag cgcatcgcgg caacaaataa cttgggaccg ctgggcgaga agaacgtcgt
     8281 ggtcacgtcg tcgccgaacc ggctcgaacg ggatttcttt acttctcctt gcgaccgccg
     8341 ccagccctcc atcgttttgc agtagagtta gtcgagtcgg cccgcggcga cgctcttggc
     8401 cagctgctgc aaccgctcgg gagaatgccc gctctgggcc ggcagcactt ccgcgtgcca
     8461 ttgccgcatt cgctcaacga tctgacagat ccgcgtctgc gccagccgga gagtctgcgc
     8521 cgcttcacgc gtcgttttcc ctcccagaat gaccgctcaa acacctcaaa atcccgcccg
     8581 ctcggcggca gccgcaagtt cgtcgccatc acaaaggccg gcagcgaaca gacttccgcc
     8641 gagctgggcc cgcggcacgc ctggcccgcc gcgcactatt ggcccctgtt cagcacgact
     8701 acgacgctgc tctggcctcc tccaacggtg gcatccaact gcaagtcact cccgaccaac
     8761 ccggcgtctc cagccgcccg ctcacccgcc agcagcgccg caaactccag cgcgccctgc
     8821 gggcagggca gtccaaacgt tgagtgctta gaatgagtca gcgaatggcc aagatcacca
     8881 gaattcacgc ccgcgagatt ctcgacagcc gcgggaagcc gacggtggag gccgaggtgc
     8941 acctggagag cggcgtcatc gggcgggcga gcgttcccag cggggcgagc acgggggccg
     9001 ccgaggcgcg cgagctccgc gacgccgatg cggcgcggta cgacggcctg ggcgtgcgcc
     9061 gcgcggtcga tcacgtgcgg cagcggattg cgccggcact catcggggcg gatgccggcg
     9121 accaggccga gatcgatcgc cggatgctcg gcctcgatgg ttcgccgaac aaggcaaacc
     9181 tgggcgctaa cgccatcctc gccgtgtcgc tggcggcagc gcgggcggcg gcgctcgcga
     9241 agggcctgcc gctctatcgg catctggccg atttggcaaa gtgctccccg cggatgccgc
     9301 tgccgatggt caacatgatc tccggcggca agcacgccgg cggcaatctc gactttcagg
     9361 acgtgctgat catcccggcg ggcgcgtccg actttcccac gcagctggag tggatcgtcc
     9421 gcatctatcg ccggctcggc cggctgctga acgaggccgg ctacgaaggc cacctcgtcg
     9481 gcgatgaagg cggttacggg ccgcggctaa agtcgaaccg cgaggccgtc gagttcgtga
     9541 tccgcgccat cgaggctgcg gggctacggc cgcgggcgga cgtgaccctc gcgctcgacg
     9601 tggcggcatc gcacttttat caaagcggcg gctactgcct ggcggccgag cagggccagc
     9661 gcctgaccag cggccagctg atcgaccgtc tggagcagtg ggccgcggaa tttcccattg
     9721 ccagcatcga ggacggcctg gccgagaacg attggcccgg ctggcaggag ctgacggcgc
     9781 ggctgggcag ccgcgtcaaa ctcgtcggcg acgacctgtt tgcaaccaac gagcggctgc
     9841 tcaagcgcgg catcgacgag cgggttgcca acgcgatcct gatcaaggtc aaccagatcg
     9901 gcacgctgag cgagacgttt gcgacgctgc ggcgagcgcg gcagcacggc tatgcctgca
     9961 tcgtcagcgc ccgcagcggc gagaccgagg acgatttcct ggccgatctc gccgtgggca
    10021 cggccgccga ctacatcaaa gtcggcagcg tcgcccgcag cgaacggctg gccaaataca
    10081 accggctgct gcgaatcgcg gaggagctgc aagattcctg aacgccgctc acatcgaccg
    10141 gctgacgacg aattcggcca tcgccgagag gacctggcgg gccgggctgg gacgaagcac
    10201 atccagccgg cggcgggcgc ggtcggcgaa gctctgggcc tgctggcggg cgtattcgac
    10261 ggcatcgtac cgctccaggt agggcgcgag cgagtcggcc cggcaatcgc cgccgccgac
    10321 caggtcgaga atcgcccgcc gatcggcggt cgtcgccaac tccagcgccc ggatgatcgg
    10381 cagcgtgggc ttttgcttct ccaaatcggt gcccagcgat ttgccggtgg tttgctcctc
    10441 gccgagcacg tcgagcaggt cgtcggcgat ctgaaaggcg atgcccaggt cgcggccaaa
    10501 accgtccagc tctatggcga ccttctcggc cgcgccggcg aacagcgccc ccagccggca
    10561 gctcaccgcg gtcagctccg ccgtcttggc ctcaatgatc tccaggtatt ccgcttcgcc
    10621 cagcaggaag ttgccgcggc tgcctttctg gcgcagctcg ccttcgcaga cgatgttcgt
    10681 cgcccggccg atcagccggc agccctgcac gctgtcgagc gtgctggcca ggtagaacgc
    10741 gtgcgtgaag aggaagtcgc ccagcagcac gctggcttcg ttgtcccagc gggcattgac
    10801 cgtcgccagg tgccggcgga tctgggcctc gtcgagcacg tcgtcgtgca ccagcgtcgc
    10861 cgtgtggatc atctccacga ccgccgccag cgtgatctgg tcccgcgtca cccgctcgcc
    10921 ggccgccttc gccgtcagga gcagcagcgc cggccgcagc cgcttgccgc ccaagaggca
    10981 gccgtagcgg accagctcat cgacgaacgg ataatcgctg cgcagctccg agcggagcag
    11041 ggcctcgacc tccgccagct cggcggcaat cggagtataa aggctgcgaa gcaattcggg
    11101 cggtttcgtc gcggtaggca tccgagtggg gcaggctttc cagcctgcca tattaggagc
    11161 atggcagaca ggaatgtctg ccccactttc acctacccgc attcagcggc cgctcttcac
    11221 ggacgaagtg cccgctcttg ccgccagctt tttcgtcaag ctgcacccgc tcgatggaca
    11281 ttccgcggtc gattgcct
//
LOCUS       DASRJB010000920        17289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2798321, whole genome shotgun sequence.
ACCESSION   DASRJB010000920 DASRJB010000000
VERSION     DASRJB010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17289
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798321"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFB80_24255"
     CDS             <1..493
                     /locus_tag="VFB80_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN36974.1"
                     /translation="PSAEEELEIIKRTTADIEVKITPTLRAEDIAALTKIVRKVPVAD
                     HVARYAMQLARLTRRQEPDAPEFVREYVQWGAGPRASQYLVLGAKARAVLLGRFFVTH
                     DDIRAIAPPVLRHRLKMNFNADAAGVSADEVIRRLLAHVPFAADEAPARGIPDVYRAA
                     NAG"
     gene            453..1352
                     /locus_tag="VFB80_24260"
     CDS             453..1352
                     /locus_tag="VFB80_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN36975.1"
                     /translation="MASPTSIEPQTLAKLKGLRLRARHIVEGYVAGLHKSPYHGFSIE
                     FAEHREYAPGDDLRYVDWKVFGRTDKVYLKQYEDETNLLCYLVLDVSESMTYKSSGAA
                     LSKLEYAQSLAAALGWLVLQQQDAVGLATFDSQLRAMIRPAGNPSHLEQLLQAMEAAT
                     PGKKTAAGPIFHELATRLTKRGIVVILSDFFDDASALAAGLKHFRHRRHDVVLLQVLD
                     PAEMDFPFQGPTEFKGLEDFPEVQADPLVIRRAYQRELESFRQSLQQACRSERIDYFL
                     VRTDQPLDVTLMNVLASRQRSVQ"
     gene            1466..3637
                     /locus_tag="VFB80_24265"
     CDS             1466..3637
                     /locus_tag="VFB80_24265"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN36976.1"
                     /translation="MSLAVIFAFWQLGSAGMLAWGLAAALPILIHLWSRRRYRQETWA
                     AMTFLLAALRKNSRRIQLEQWILLAVRTGILALFALALADPQLSTLGSWIGGAGGGQT
                     HVVLVIDGSYSMDYRREDKPRFAAAKELAKQLVQSGQQGDGYTLVLMGEPPRAVIAQP
                     AFDAEDVLEELNNLEMPHAGAGLPAALAEVETILRQAAERQQRLTRRRVCFFTDLQQT
                     TWGEVNSPDCRARLGQIQSLASLELIDLGQAGESNLAVARLEIDQPLVAARSEVQIQA
                     DIQSYSREDRPRQAVEVLVDGQRIADERVDVPAGGRTTVAVTHKFDVPGEHVVEMRLA
                     DDALPLDNRRWLSVPVRETIRVLCVGGRPGETRHIALALAPRKQTAGPLEIVEAPESR
                     LLEGDLAQFDCVFLADIGRFSRDEAGVLHRFVSRGGGLIVFLGDQTQAENYNQLLVDD
                     AAQRVLPARLLEPAPTAASAYRLNPLEYRHPIVGPFSGFTQAGLLTTPIWKYVKLAPL
                     EGSKTAAAFDNGDPAIVEARVGRGRTILVATAASPLSVDRASDPPSPWSALAEWPSFP
                     PLVHEMLKLALSGRGESRNLLVGDELTGLVPQTALSEAVLLAGPHDLQERLPVAVEGG
                     EGRWSFSGTGASGEYSARLGQAVQRFAVNVNPRESDLARFDPELLPSQLSREPIPQGE
                     DVSPALASNDAASYFRWLLGGVLALLFLEPVLAWRFGRGRG"
     gene            3634..6042
                     /locus_tag="VFB80_24270"
     CDS             3634..6042
                     /locus_tag="VFB80_24270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36977.1"
                     /translation="MNDAWRQVERWLVGENLSADPGGLETSIQPTWTLAPGLVALVLL
                     ATAAMMLLVYWNESATADRGWKVLLAGLRTLLVGLILLMLFGWTVQRHRTDLPDVVIV
                     LDDSASMSLADTYDDASLLTEIKRRLAAAKLPEQTRLNLAKTLLLENEANLLAELGRR
                     ANLRFYLAGGSARAIGGEPEQVRSALLQAAADQPASRLGNCLRDVLEAQRGRPTAAVI
                     FLTDGVTTDGKSLLEAAQYARRKTVPLFFVGLGSDRPARDLRLADLLADEAAFVGDLL
                     HFDVKLFGEGLSGQAAVQLKRSGESEVLAEEKVTLDKAGAAQSIRLAHRAEKPGEFEY
                     VVEVVPHAGEANTDNNRLTRKVTVREETIRVLLVQAYPNYEFRFLKQMLVRELNANQP
                     AEGKAQGVRTVLQEADLEYVETDKTAERVFPVSREELFQYDVLIFGDVNPSLLSPSIM
                     NNIYEFATVRGGGVIFMAGPRYTPLAFRDTPLAPLLPMNLDTVRLPDPDATIAESFRP
                     KLTALGLASPITQLADSPAANERLWQGELAPLRWFASIQDLRPGVRVLAEHPTKRSDS
                     GQPLPIITLQFVGAGKVVFHATDETHRWRFRVGDQYFARYWVQTIRYLCRSKLLAGNR
                     SAELTTDREEYRRGDAVGLRVRFLDDRLAPAQDDGVMVVVARSGGERRNITLRRLASD
                     RGIFEGTAGPLPDGQYRAWLAAPTLEGQPPARAFTVVAPPGELARTQMDAAEMREAAK
                     ISVGKYYTFSTAGGLLADLPRGRQVRIESLPPKPIWNAPLLAALFVALIAAEWLLRKR
                     VGML"
     gene            6133..9669
                     /locus_tag="VFB80_24275"
     CDS             6133..9669
                     /locus_tag="VFB80_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36978.1"
                     /translation="MSASLRQRVESVQRGARRLVWQYGLARFAAAVIALVLGVGLIDY
                     LIRLHDPALRWLFSAVALAGAAWFFLRLAWPAIAFRSNLVSAARRIELCFPMLGERLS
                     SAMAFLTQSAGDPTAGSAGLRRAVVAEAEALAAGLDFRAVLDRRAPRRAGLVAAVVLG
                     VAGLVCAANPAAAGLALARLAQPWRELPWPRRHELAFVAVPERLASGSNLELALVDRH
                     GRLPESVELLLRHEGRIESRPMKLLGERMVARLDNVTRGFDYRARGGDDDTMPWTELT
                     VVEPPKIVELSVEVEPPAYAGLPARQEGRIAKALIGSKLRVRGRLDKPIREALAKVET
                     AGLNVPAVQVSADGRSFRAPAQGQWLVERSGAFWFELTDESGVVFGRDTRIELHAAAD
                     APPSIAWESPADHSFVTPRAIVPVRGLVKDDLAIRGIQLRYLRPGQSDEEQVLPLYVG
                     QAASLPGQTATNGRGFEGDSRAIDYAWDLAQLAGLAPGDVLAARITAEDHKPQLATSI
                     VHRLTIITDEELTSRITQRQGAILSQLAEALRMARQCREQTAALEARLLESKQLAAGD
                     LNHLQSAQHNQRQVQRLLGAGPEGVEGQIVALLAELAANRVENHASGQRLNELLAQVR
                     ALNLQPLPAIDQQLGEAFKTAREALDAAPCGEGPAEGVAHTLHEAGTKQDEVVQALER
                     MVGTLSEWDSFSRLAREIGQIRSEEERLARDTDELIVKAAAAAPPAAAAGNLSRQQVE
                     LGRQLDKVQTRMEEMLTRLQATDPLAAGTLADALNTARSLAIGGQMREAAARLRQLQL
                     GEARQTEQLVLDGLKQLLETLSLRRDYELARTVGSLKSASAALGGMLSRSAQVQADLT
                     AAAALPADEKRRRELQRLQRELEKLAQESQELGRKLQRLQAPRPAAALSQAAASSGGA
                     AQAAGGGDAPQAQQQGADAHRRLEEAQQELAQAIAEAEQELLQEQLARVEQLVAGIAA
                     RQKNVVSEIQRLNAGQPTPAELRNLAAEEQLLADELGQLRPKLEAAEAFGFALDGAAE
                     QMRRAGGMVQRGETGPAAQDAAQSALTRLNQILEALRPENPAGEQAPGQQQPGQQANS
                     NPSGMSAELKLLQLLQQEVNRRTTELEARRNRNGSLTADEQAERDSLAREQGRLADMV
                     LNMIRASAERPEDNPDLIPKKPPQKDKDEEQP"
     gene            9666..10340
                     /locus_tag="VFB80_24280"
     CDS             9666..10340
                     /locus_tag="VFB80_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36979.1"
                     /translation="MSRSPASGALGLLMLALPALAADEPPKPPSKPPSKPSALDQQLL
                     DGLDRELLQGLPGGIKPANPADKPALDKAGAIQSENPLALIAERMRKVEARLAQQDTS
                     QETQDAQKQILAELAKLLEQARQQQAQKKPGGSGKGSNQAGAGSGNPVPAPPRDSTNR
                     IEQGTKETVETADVKDLLRRIWGHLPDKLRGEMQASLSEQFLPKYERLIEEYYKRLAE
                     ERPGAP"
     gene            10451..11506
                     /locus_tag="VFB80_24285"
     CDS             10451..11506
                     /locus_tag="VFB80_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN36980.1"
                     /translation="MFRLPRRDFLTGTAAAFGAAAFPTALFAQRSPTDTERTAGLLIK
                     RSTQDAIDRGLVWLAARQNDDGSFGGNGYSRNVAVVSLAGMAFLSGGHTPGRGEYGQV
                     VNRCVSYILQAADESGFVCEPLYTSHGPMYDHGFGTLFLAEVYGMSPDSLIRDKLAKA
                     VRLIVTTQNPDGGWRYQPKASEADISVTICQVMALRAARNAGIYVPNETIDRCVDYVK
                     RSQNADGGFMYMISGGPSRFPRSAAGVVALYSAGIYEGEEIRKGLEYLEQHLPAAEDF
                     SRDTHAMYGHYYAVQAMWQAGGEHWQKWYPAVHDVLLKTQQEDGSWMDLICPEYGTAM
                     AEIILQMPNNYLPIFQR"
     gene            12179..13474
                     /locus_tag="VFB80_24290"
     CDS             12179..13474
                     /locus_tag="VFB80_24290"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HZN36981.1"
                     /translation="MKSIAKLRGIISILAVVALAQVPGLAQPLAFPIAEAPFPAQVAG
                     IDGQWNISLKSAGKIRVLRADDLAYWGAYRDVEAGPQLILTDGSVVRSDLLLLNDKQL
                     VIGDATGLGRGLWDESTLPRSALRAIVWQPPAAAAERDRLLATLVRENGNSDRLTLLG
                     GETLSGLLTAAPPAGRFAPEEIKPGSEFFEFARPGQAEPLRIPSARVVAIRLAQPVQP
                     AAVSGMSAWLGLADGSFMKVRKITVQGDVVTLALAAGGQLTTTLTGRKDASRKFWDEI
                     TCLEPAAAGLAWLSDLPGLGYRHIPFLSVQRPLGLDSCVLGSRLRAGGAVHRKGLGMP
                     TASRIAVRAAGYRRFEAQIALDEAAGTEGSAIFKVLLETAPGEWRTAYESPPVRGGEL
                     PRQVAVELAGASRIALVVDFAERGDVCDWADWLEARLVK"
     gene            13626..13826
                     /gene="rpsU"
                     /locus_tag="VFB80_24295"
     CDS             13626..13826
                     /gene="rpsU"
                     /locus_tag="VFB80_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZN36982.1"
                     /translation="MVKLIVRDKESIQEAVRRFRKLVERSGIKKEMRRREYYEKPSEI
                     RRRARLRAERRARRSTMKPVGM"
     gene            14161..14391
                     /gene="csrA"
                     /locus_tag="VFB80_24300"
     CDS             14161..14391
                     /gene="csrA"
                     /locus_tag="VFB80_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HZN36983.1"
                     /translation="MLVLSRHRDESIMIGDDIVVTIVDIRGDKVRLGIEAPTAIPVHR
                     QEVYEAIQRENRKATQLKPEDARDVGKPNPPR"
     gene            14708..14833
                     /locus_tag="VFB80_24305"
     CDS             14708..14833
                     /locus_tag="VFB80_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36984.1"
                     /translation="MTHDPWLARADAGELSGKQVIGERAADPLWRLRPERTPPGT"
     gene            14890..16299
                     /locus_tag="VFB80_24310"
     CDS             14890..16299
                     /locus_tag="VFB80_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZN36985.1"
                     /translation="MSRINTNVSSLIAQKILGRTNNQLQEALNRLSTGLRINVGKDDP
                     AGLIASENLRRDIASANKAISNTERANQLIGTADSALGQISGLLIDIRGLVVEAANTG
                     VLSDEQLAANQLQVDSSLEAIDRISQVTTFQGRKLLDGSLDFQITYTAGGATVEDLNI
                     DQANLGTAGSVAVDIQISVAATQALITNAALPLTDDLVVKIAGQKGSEVFTFELGATV
                     TQIVDAVNLVSDATGVQATDNGGTLELESTDYGSAAFVDVEVLEEGALGTFEAGLSAT
                     REVGIDIAATVNGVTANGAGNTLSINTSTLDLSLTVADGSAVAVQFTIDGGGALFQLG
                     PQIVSNQQARIGIGSVNTGELGGVPGRLYQLRSGNDADLTTDARTASLIVDVAINKVT
                     GLRGRLGAFQRATLETNIASLQDTVTNLAEAESSIRDADFAAETARLTRAQILVQSGT
                     SVLAIANQNPQNVLALLRG"
     gene            16563..>17289
                     /locus_tag="VFB80_24315"
     CDS             16563..>17289
                     /locus_tag="VFB80_24315"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HZN36986.1"
                     /translation="MAGIQSSTGIITGIPIQDTVDKLIALQAQPRDRLLARQKVLQAE
                     QAAIGDLMALTLGVQLAVRRLKNLDLFSARNATSSNPALLTATASSEAIPGQYQFVPL
                     RPAQSHHALSSGLAAPDAALGGGTFTVRYGGQVDRGISLADLNSGAGVSRGKIKITDR
                     SGAWAIIDLRYAQTIDDVLAAINASDEIAVRATAAGDHLVLSDSSGGTGNLRVQEVGD
                     GTTAADLGLDGINVAAGEAGGTDL"
BASE COUNT         3048 a         5703 c         5799 g         2739 t
ORIGIN      
        1 cccgtcggcc gaggaagaac tggagatcat caagcggacg acggccgaca tcgaggtcaa
       61 gatcacgccg acgctgcgtg ctgaagacat cgccgcactg accaaaattg tccgcaaggt
      121 gccggtggcc gatcacgtcg cccgctacgc gatgcagctc gcccgcctga cacggcggca
      181 agagccggac gcgccggagt tcgtccgcga atacgtgcag tggggcgcag gcccgcgtgc
      241 cagccaatac ctggtgctcg gagcgaaggc tcgggcggtg ttgctgggcc ggttctttgt
      301 cacgcacgac gacatccggg cgatcgcccc accggtgctg cggcaccggc tgaagatgaa
      361 tttcaacgcc gatgcggcgg gagtttcggc cgacgaggtg atccgccgcc tgctggctca
      421 cgttcctttc gccgccgacg aggctcccgc acgtggcatc cccgacgtct atcgagccgc
      481 aaacgctggc taagctcaag ggcctccgcc tgcgggctcg ccacatcgtg gagggctacg
      541 tcgcggggct gcacaagagc ccgtatcacg gcttctcgat cgagttcgcc gagcaccgcg
      601 agtacgcgcc gggcgacgac ctgcggtacg tcgattggaa ggtctttggc cgcaccgaca
      661 aggtttacct gaagcagtac gaagacgaga cgaacctgct ctgctacctc gtcctggacg
      721 tgagcgagag catgacgtac aagagcagcg gcgcggcgct ttcgaagctc gaatatgccc
      781 agtcgctggc ggcggcactc ggctggctcg ttttgcagca gcaagacgcc gtcggcctgg
      841 cgaccttcga cagccagctg cgggcgatga tccgccccgc cggcaaccca tcgcatctgg
      901 agcagctcct gcaagcgatg gaggcggcta cgcccggcaa gaagaccgcc gccggcccaa
      961 tcttccacga gctggcgacg cgactgacca agcggggcat cgtggtgatt ctcagcgact
     1021 tcttcgacga cgcatccgca ctcgcggcgg gcctcaagca tttccgccac cgccgtcacg
     1081 acgtcgtcct gctgcaagtg ctcgacccgg ccgaaatgga cttccccttt caaggtccga
     1141 ccgaattcaa aggcctagaa gactttcccg aagtccaggc cgacccgctc gtcatccgcc
     1201 gcgcctacca gcgcgagctg gaatccttcc gccaatcgct ccagcaagcc tgccgcagcg
     1261 agcggatcga ctattttctc gtgcggaccg atcagccgct ggacgtgacg ctgatgaacg
     1321 tgctggcgag ccggcagagg agcgtgcagt gaaagcaaaa atcaaagaac caaagaaccg
     1381 aagaaccaaa ccaaacgaag ggaagtcact ccgccacctt tggttctttg gttcttcgct
     1441 tcttgcaatt tgataccaga ttggcatgtc cttggcagtc attttcgctt tctggcagct
     1501 cggcagcgcc ggcatgttgg cgtgggggct ggcggctgcg ctgccgattc tgattcacct
     1561 gtggagccgc cgccgttatc ggcaggaaac gtgggcggcg atgacgtttc tgctggcggc
     1621 cttgcgcaag aactcacggc ggattcaact ggagcagtgg atcctgctcg cagtgcgcac
     1681 ggggattctc gcgctgttcg cgctggcctt ggccgatccg cagctctcca cgctcggttc
     1741 ctggatcggc ggcgcgggag gcggccagac gcacgtggtg ctcgtcatcg acggctccta
     1801 ctcgatggac taccgccgcg aggacaagcc gcggttcgcc gcagccaagg agctcgccaa
     1861 acaacttgtc caaagcggcc agcagggcga cggctatacg ctcgtgctta tgggcgagcc
     1921 gccgcgggct gtgatcgccc agccggcgtt cgacgcggag gacgtgctgg aagagctgaa
     1981 caacctcgaa atgccgcacg ccggcgctgg gttgcccgcc gcgctggcgg aggtcgaaac
     2041 gatcctccgg caggccgccg agcggcagca gcgcctgacg cggcggcgag tctgcttctt
     2101 tacggacctg cagcagacga cctggggcga agtcaactcg cctgattgcc gggcgcggct
     2161 aggccagatt cagtcgctgg cctcgctgga gctgatcgac ctgggccagg ccggcgaatc
     2221 gaacctcgcc gtcgcgaggc tcgagatcga tcagccgttg gtcgccgctc gcagcgaagt
     2281 gcagatccag gccgacattc aaagctacag ccgtgaggac cgcccgcgtc aggccgtgga
     2341 ggtgctcgtc gatggccagc ggatcgcgga cgaacgcgtc gatgtgcccg ccgggggccg
     2401 cacgacggtc gcggtgacgc acaagtttga cgttccggga gagcatgttg tcgaaatgcg
     2461 tctggccgac gacgccctgc cgctcgacaa ccgccgttgg ctcagcgtgc cggtgcgcga
     2521 gacgattcgc gtcctctgcg tcggcggccg gcccggcgaa acgcgccaca tcgcgctggc
     2581 cctcgcaccg cggaagcaga ccgccgggcc gctggaaatc gtcgaggccc ccgaaagccg
     2641 gctgctcgaa ggcgacctgg cccagttcga ctgcgtcttt ctggctgaca ttggccgctt
     2701 cagccgcgac gaggcaggcg tcttgcaccg cttcgtcagt cgcggcggtg gcctgattgt
     2761 gttcctcggt gatcagacgc aggccgagaa ctacaaccaa ttgctggtcg atgacgcggc
     2821 gcagcgcgtg ctgcccgccc gactgctgga gcccgcgccg acggccgcga gcgcgtatcg
     2881 cctgaacccg ctggagtatc gccacccaat cgtcgggccg ttcagcgggt tcacccaagc
     2941 cgggctgctg accacgccga tctggaagta cgtcaagctc gcgccgctcg aggggtcgaa
     3001 gacggccgcc gccttcgaca acggtgaccc ggcaatcgtc gaagcccgcg tcggccgcgg
     3061 tcggaccatc ctcgtggcca ccgccgcatc gccgctgtcg gtcgatcggg cgagcgatcc
     3121 gccgtcgccc tggtcggccc tcgccgagtg gcccagcttt ccgccgctgg tgcacgagat
     3181 gctgaagctg gctttgtcgg gccgcggcga gagccgcaat ctgctcgtcg gcgacgaact
     3241 gaccggtctc gtgccgcaaa ctgcactcag cgaagcagtg ctgcttgcgg ggccgcatga
     3301 tcttcaggag cggctgcctg tggccgtgga gggcggcgag gggcggtgga gcttctcggg
     3361 tacgggggcc agcggcgagt attcggcccg gctaggtcag gcggtgcagc ggttcgccgt
     3421 gaacgttaat ccgcgcgaga gcgatttggc ccgcttcgat cctgaattgc tgcccagcca
     3481 gctcagccgc gagccgatcc cgcagggcga agatgtgtcg ccggcgctgg cgtcgaacga
     3541 tgccgcttcc tatttccgct ggctgctggg gggcgtgctg gcgctgctct tcctcgagcc
     3601 ggtcctcgcc tggcgttttg ggaggggccg cggatgaacg acgcctggcg gcaagtcgag
     3661 cgctggctcg tcggcgagaa tctctcggcc gatccgggcg gcttggagac gtcgatccag
     3721 ccgacctgga cgctcgcgcc gggcctcgtc gcgctggtgc tgctggcgac cgcggccatg
     3781 atgctgctgg tctattggaa cgaaagcgcc accgcggacc gcggctggaa ggtcttgctg
     3841 gccggcctgc ggacgctgct cgtcgggctg atcctgctga tgctcttcgg ctggacggtc
     3901 cagcggcatc gcaccgactt gcccgacgtg gtgatcgtcc tcgacgactc ggccagcatg
     3961 tcgctggccg acacctacga cgacgcctcg ctgctcacag aaatcaagcg ccgcctggcc
     4021 gccgcgaagc tgcccgagca gacgcggctg aatctggcca aaacgctgct gctggaaaac
     4081 gaggcgaatc tgctcgcgga acttggcagg cgggccaacc tgcggttcta tctggcgggc
     4141 ggatcggccc gcgcgatcgg gggcgagccg gagcaggttc gctcggcgct gctccaggca
     4201 gccgccgatc aaccggcgag ccggctgggc aactgtctcc gcgacgtgct cgaagcgcag
     4261 cgcggccggc cgacggcggc ggtcattttc ctgaccgacg gcgtgacgac cgatggcaaa
     4321 tcgctgctgg aagccgccca atatgcccgc cgcaagaccg tcccgctgtt cttcgtcggc
     4381 ctgggcagcg accgcccggc tcgcgacctg cggctggctg atctgctggc cgacgaagcc
     4441 gcattcgtcg gcgacctgct gcacttcgac gtcaagctgt tcggcgaggg cctcagcggc
     4501 caggcggccg tgcagctcaa acgcagcggc gagagcgaag tgctcgcgga agaaaaggtg
     4561 acgctcgaca aggccggcgc ggcgcaatcg atccgcctcg cccaccgcgc ggaaaagccg
     4621 ggcgaattcg agtacgtcgt cgaagtcgtg ccgcacgcgg gcgaggccaa caccgacaac
     4681 aaccgcctca cgcgaaaagt gacggtccgg gaagagacga tccgcgtgct cctggtgcag
     4741 gcctatccga actacgagtt ccgctttctg aagcagatgc tggtccgcga actgaacgcc
     4801 aatcagccgg cggaaggcaa ggctcagggc gtgcggacgg tgctccagga agccgacctg
     4861 gaatatgtcg agaccgacaa gaccgccgag cgcgtcttcc cggtcagccg cgaggaattg
     4921 ttccaatacg acgtgctgat tttcggcgac gtgaatccgt cgctgctcag cccgtcgatc
     4981 atgaacaaca tttacgagtt cgcgaccgtc cgcggcggcg gcgtcatttt catggcgggc
     5041 ccgcggtaca cgccgctggc cttccgcgac acgccgctgg cgccgctctt gccgatgaac
     5101 ttggacaccg tgcggctgcc cgatcccgat gcaacgatcg ccgagtcgtt tcgtcccaag
     5161 ctgacggcgc tgggtctggc cagtccgatc acgcagctcg ccgactcgcc cgcggctaac
     5221 gagcgattat ggcagggcga gctcgcgccg ctgcggtggt ttgcgtccat ccaggacctg
     5281 cggccgggtg tccgcgtgct ggccgagcat ccgaccaaac gctccgacag cgggcagccg
     5341 ctgccgatca tcacgctgca gttcgtcggc gcgggcaagg tcgtgtttca tgcgacggac
     5401 gaaacgcacc gctggcggtt ccgggtgggc gaccagtatt tcgcccgcta ttgggtgcag
     5461 acgatccgct atctctgccg ctccaagctt ctggccggca accgctcggc ggagctgacc
     5521 accgaccgtg aggaataccg ccgaggcgat gccgtgggcc tgcgggtacg gttcctcgac
     5581 gaccgtctcg ctccggcaca ggacgacggc gtgatggtcg tcgtggcccg ctccggcggc
     5641 gagcgccgca acatcacgct gcgccgcctg gcatcggacc gcgggatctt cgaggggacg
     5701 gccggcccgc tccccgacgg gcaataccgc gcttggctgg ccgcgccgac gctcgagggt
     5761 cagccgccgg cgagggcctt caccgtcgtc gccccgccgg gcgaattggc ccgcacccag
     5821 atggacgccg ccgaaatgcg cgaagcggcg aaaatctcgg tcggcaagta ctacaccttc
     5881 agcacggcgg gcggcttgct tgccgatttg ccccgcggcc ggcaagtgcg gatcgaatcg
     5941 ctgccgccca agccgatctg gaacgcacca ctactggcgg ccctgttcgt ggcgctcatc
     6001 gcggccgaat ggctgctgcg gaagcgggtg ggaatgctgt aggtaagcgg taggtggtga
     6061 gtggtaggca gaaaccgtag gggacggcgc ttcgtctagt catagaagcc gttgtcgtgc
     6121 gacaattact gcatgtccgc atccctccgc caacgcgtcg aatccgtcca gcgcggggct
     6181 cggcggctcg tctggcagta cggcctggcc cggtttgcgg ctgccgtcat cgccctggtt
     6241 ctcggcgtgg ggcttatcga ctatctcatc cgcctgcacg atccggccct gcggtggctg
     6301 ttctcggcag tggctctcgc gggggccgcc tggttcttcc tgcggctggc ctggccggcg
     6361 attgcgtttc ggtccaacct ggtgtccgcg gcgcggcgga tcgagctgtg ctttccgatg
     6421 ctgggcgagc ggctctccag cgcgatggcg ttcctgacgc aatcggcggg cgatccgacc
     6481 gcgggctcgg ccggcctgcg gcgggcggtg gtcgcagagg cggaagcctt ggcggccggt
     6541 ctcgatttcc gcgcggtgct cgaccgccgc gctccgcggc gggcggggct cgtggctgcg
     6601 gtcgtgctcg gcgtcgcggg cctggtctgt gcggcgaatc ctgcggcggc cggactggcc
     6661 ctcgcccgcc tggctcagcc ctggcgcgaa ttgccttggc cgcggcgaca cgagctggcg
     6721 ttcgtggccg tgccggagcg actggccagc ggcagcaacc tggaactggc gctggttgat
     6781 cgtcacgggc ggctgccgga gtcggtggag ctgctgctgc gccacgaggg gagaatcgag
     6841 tcgcggccca tgaagctgct cggcgagcga atggtggcgc ggctcgacaa cgtcacccgc
     6901 ggattcgact accgggcccg cggcggcgac gacgatacga tgccctggac cgagctgacc
     6961 gtcgtcgaac cgccgaagat cgttgagctg tccgttgagg ttgagccgcc cgcctatgcc
     7021 ggactgcccg cccggcagga agggcggatt gcaaaagcgc tgatcggttc gaaacttcgc
     7081 gtccgcggtc ggctcgacaa gccgatccgc gaggccctgg cgaaagtgga aacagcgggc
     7141 ctgaacgtgc ccgcggtgca ggtcagcgcc gatggccgca gcttcagagc gccggcccag
     7201 gggcagtggc ttgtcgagcg atcgggcgct ttctggttcg agctgaccga cgaaagcggc
     7261 gtcgtctttg gccgcgacac gcgcatcgag ctgcacgccg cggccgatgc accgccgtcg
     7321 atcgcctggg agtcgccggc cgaccactcg ttcgtaacgc cgcgggcgat cgtgccggtc
     7381 cgtggcctgg tgaaagacga tctggccatc cgcggcattc agctccgcta tctccgcccc
     7441 ggccagagcg acgaagaaca ggtgctgccg ctctacgtag ggcaagctgc cagcttgccc
     7501 ggccagaccg cgaccaatgg tcgcggcttt gagggagaca gccgcgcgat cgattatgcc
     7561 tgggatctcg cccagctggc cggcctcgcg ccgggagacg tgctcgcggc ccgcatcacc
     7621 gccgaggatc ataagccgca gctggcaacg agcatcgtcc atcgcctgac gatcatcacc
     7681 gacgaagaac tgaccagccg gatcacgcag cggcagggcg cgattctgag ccagctcgcc
     7741 gaggccttgc ggatggcccg ccaatgccgc gagcagaccg ccgccctcga agcccgtctg
     7801 ctcgaatcca agcaactcgc cgcgggcgac ctcaaccacc tgcagagcgc ccagcacaac
     7861 cagcggcagg tccagcggct gctgggcgca ggccccgagg gcgtggaggg ccagatcgtc
     7921 gccctgctgg cggagctggc ggcaaatcgc gtcgaaaatc atgccagcgg gcagaggctg
     7981 aacgagctgc tcgcgcaagt ccgcgcgctc aacctgcagc ctttgccggc gatcgatcag
     8041 cagctcggcg aggcgttcaa gacggcccgg gaggcgctcg atgccgcgcc gtgcggcgag
     8101 ggaccggcgg aaggcgtcgc ccacactctg cacgaggccg ggacaaaaca ggatgaggtc
     8161 gtgcaagccc tggagcggat ggtcggcacg ctctccgagt gggacagctt cagccggctg
     8221 gcgcgggaga tcggccagat tcgcagcgaa gaggagcggc tggcgcggga taccgacgag
     8281 ctgatcgtga aagctgcggc cgcggccccg ccggccgccg ccgccgggaa tctctcgcgg
     8341 cagcaggtcg aactgggccg gcagctcgac aaggtgcaga cccggatgga ggaaatgctc
     8401 acccgtctgc aagccaccga tccgctggcc gctgggacgc tggccgacgc gctgaacacg
     8461 gcccgcagcc tggcaatcgg cgggcagatg cgcgaggcgg ctgctcggct caggcaattg
     8521 cagctgggcg aggcgcggca gaccgaacag ctggtgctgg atggtctcaa gcaattgttg
     8581 gaaacgctct cgctccgccg cgattacgag ctggctcgca cggtcggctc gctgaagagc
     8641 gcttcggccg ccctcggtgg gatgctctcg cggagtgcgc aagtgcaggc tgacctgact
     8701 gctgccgccg cgctgccggc cgatgagaag cgccgccgcg aattgcagcg gctgcagcgt
     8761 gagctcgaga agctggccca ggaaagccag gagctgggtc gcaaacttca acgattacag
     8821 gccccgcggc cggcggcggc gctgagccag gcggcagcga gcagcggcgg cgcggcacag
     8881 gcggcgggcg gcggcgacgc cccgcaggcc cagcaacaag gcgccgacgc tcaccgccgc
     8941 ctggaagagg ctcagcaaga gctggcccag gcgatcgccg aagcggagca ggagttgctg
     9001 caagagcagc tcgcccgcgt cgagcagctc gtcgcgggaa tcgccgcgcg gcaaaagaac
     9061 gtcgtgtccg agattcaaag gctgaatgcg gggcaaccga cgccggctga gctgcgcaat
     9121 ctggcggccg aagaacagct cttggccgat gagcttggcc agttgcggcc gaagctggag
     9181 gcggccgaag cgtttggatt cgccctggat ggcgctgcgg agcagatgcg gcgggccggc
     9241 ggcatggttc agcgcggcga gactgggccc gcggcacagg acgcagccca gtcggcgctc
     9301 acgcggctga accagattct cgaagcgctg cggcccgaaa atccggccgg cgagcaggcg
     9361 ccgggccagc agcaaccagg gcagcaagcc aacagcaacc cctccggcat gtcggccgag
     9421 ctgaagttgc tccagctcct gcagcaggaa gtcaaccgcc gcaccacgga gctcgaagcc
     9481 aggcggaaca ggaacggcag tctcaccgcc gacgaacagg ccgagcgaga ttcgctggct
     9541 cgcgaacagg gccgccttgc cgatatggta ctgaacatga tccgcgcttc ggccgagcgg
     9601 ccggaggaca atcccgatct gattccgaaa aagccgccgc agaaggacaa ggacgaggag
     9661 cagccatgag tcgttcaccc gcgtccggcg cgctgggact gctgatgctg gctctgccgg
     9721 cgctggctgc cgacgagccg cccaagccgc ccagcaagcc gcccagcaaa ccgtcggcgc
     9781 tcgaccagca attgctggac gggctcgatc gcgaattgct ccaaggattg cccggaggca
     9841 tcaagccggc gaatcccgcc gacaaacctg cgctcgataa ggcgggcgct attcagtctg
     9901 aaaatcctct cgccctgatc gccgagcgga tgcgaaaagt ggaagcgcga ctggcgcagc
     9961 aagatacgtc gcaggagacg caagacgctc agaagcagat tctggcggag ctcgcaaagt
    10021 tgctcgagca ggcccgccag cagcaagccc agaaaaagcc gggcggttcg ggcaagggct
    10081 cgaatcaagc cggcgccggc agcggcaatc ccgtccccgc ccccccgcgc gacagcacca
    10141 accggatcga gcagggcacc aaagaaaccg tcgaaaccgc cgatgtgaaa gacctgctcc
    10201 gccgcatctg gggccacctg cccgacaagc tccgcggcga gatgcaagca tcgctgagcg
    10261 agcagttcct gccgaaatac gaacgcctca tcgaggaata ctataaaagg ctggcggagg
    10321 agcggccggg tgcgccataa tctcgtcgta gagtaagcgt cctcgcttgc tcaaagccgc
    10381 gaccagtggt cgcggtgcac aagtgaggac gcttatgcta caagcgggcg ggccgaaagg
    10441 aaggcaacgc atgtttcgat tgccacgtcg cgatttcctc acaggcacgg cggccgcgtt
    10501 tggcgcagcg gcatttccga ccgcgctctt tgcccagcga tcgcccaccg acaccgaacg
    10561 cacggcggga ctgctcatca agcgttcgac gcaggacgcc atcgaccgcg gcctcgtttg
    10621 gctcgcggcg cggcagaacg acgacggctc ctttggcggc aacggctaca gccgcaatgt
    10681 ggcggtcgtc agcctggcgg gcatggcgtt tctctccggc gggcacacgc cgggccgcgg
    10741 cgaatacggc caggttgtca accgctgcgt gagctacatc ctgcaggccg ccgacgaaag
    10801 cggcttcgtc tgcgagccgc tctacaccag ccacggcccg atgtacgacc acggcttcgg
    10861 cacgctgttt ctggccgaag tttatggcat gtcgcccgac agcctgatcc gcgacaagct
    10921 ggccaaggcc gtgcggctga tcgtgacgac gcaaaacccc gacggcggct ggcggtatca
    10981 gccgaaggcc agcgaggcgg acatctcggt gacgatctgc caggtgatgg ccctccgcgc
    11041 ggcccgcaac gccggcatct acgtccccaa cgagacgatc gaccgctgcg tcgattacgt
    11101 caagcgcagc cagaacgccg acggcggctt catgtacatg atcagcggcg gacccagccg
    11161 ctttccgcgg agcgccgccg gcgtggtcgc gctgtacagc gccggcatct acgaaggcga
    11221 ggaaattcgc aagggcctgg aatatctgga gcagcatctg ccggcggccg aggatttcag
    11281 ccgcgacacg cacgcgatgt acggccacta ttacgcggtg caggcgatgt ggcaggccgg
    11341 cggcgagcac tggcagaagt ggtaccccgc cgtgcacgac gtgctgctca agacgcagca
    11401 ggaagacggc tcctggatgg acctcatctg cccggaatac ggcacggcaa tggccgagat
    11461 catcctgcag atgccgaaca actacctccc cattttccag cgctaatggc gagggcgtcg
    11521 cggtcccatc gtgtcggctc gcaggcgtat cgtgggctgc taaaaactcg ttcgccagca
    11581 agcacaatga gcatgtcggt tttattcccg aggtttccgg ttcgacgtga aagggtccag
    11641 tggcctacta gccgcggagc ggcgacaggg agtagccgtg ggcgttagcc cacggaccga
    11701 tgagcgattg gaaaaaacca agccccggag gggcgacacc agggcgcgag gtgtcgcccc
    11761 tccggggctt acgatcgcgc tggagcacgc aatccgtggg ctgacggcta ttgcctaccg
    11821 cccctccggg gctgcgccaa cgatccgtcc cggtcatcgc gccttgggcc agaacgaatt
    11881 cagctcccca accaacaacc tccagcaacg agtttttagc agcccaggcg aactgtgaac
    11941 atcggtactg tatttgcagt aaagttctct ttggcaaacg gtccgccgtt ttggcaaacc
    12001 ggtcgccggt gagccgactt agcggcccga aaaaaagagt tctctaaccg taataagtac
    12061 tacttaacgg ggtcgcgaaa aaacgcccga agtcaatggc gcctcatgag ttgtggccga
    12121 atggggcaga ttcagctcta cccaaccagg gggctgataa tgggacaagt ccccagcgat
    12181 gaagtcgatc gcaaagctac gcggcatcat cagcatattg gcggtcgtcg cgctggcgca
    12241 ggttcctggc ctggctcagc cgctggcgtt tccgatcgcc gaagctccct ttccggcgca
    12301 ggtggcaggg atcgacggcc agtggaacat cagcctcaaa tcggccggca agattcgcgt
    12361 cctgcgagcc gacgacctgg cgtactgggg cgcttaccgc gacgtcgagg ccggcccgca
    12421 gcttatcctg acggacggca gcgtggtgcg ttcggacctg ctgctcttga acgacaagca
    12481 gctggtgatc ggcgacgcga cgggcttggg gcgcggcctg tgggatgaat cgacgctgcc
    12541 gaggtcggcc ctgcgggcca tcgtctggca gccgccggct gctgcggccg agcgcgacag
    12601 gctcctcgcc actctggttc gcgaaaacgg gaacagcgat cgactgacgc tgctgggcgg
    12661 cgaaacgttg tcaggcctgc tgacggccgc gccacctgcc gggcggtttg ccccggagga
    12721 gatcaagccg ggcagcgagt tctttgaatt cgctcgccct ggccaggctg aaccgctgag
    12781 aattccctcg gcccgcgtgg tggcgattcg cctcgcgcag ccggtacaac cggcggcggt
    12841 cagcggaatg tctgcctggc tggggcttgc ggatggctcc ttcatgaagg tccggaagat
    12901 caccgttcag ggcgacgtgg tgaccctggc cctcgccgcg ggcggacaac tgaccacgac
    12961 gctgaccggc cgcaaagatg cctcacgtaa attctgggat gagattacct gtctggagcc
    13021 ggctgccgcc gggctggcct ggctgtccga cctgcccgga ctgggttacc ggcatattcc
    13081 attcctgagc gtgcagcggc cgctgggctt ggactcatgc gtcctcggca gccggttgcg
    13141 ggcgggcggc gcggtgcacc gcaaggggct gggaatgccc acggcctcga gaattgccgt
    13201 tagggcggcc ggctatcgcc gctttgaagc gcagattgct ctcgatgaag ccgcggggac
    13261 cgagggtagc gcgatcttca aggtgttgct cgagactgcc ccgggagagt ggcgcaccgc
    13321 ctacgagagt ccgcccgtaa gaggcggcga actgccgcgg caggtcgcgg ttgaactggc
    13381 gggcgcctcg cggatcgcgc tggtggtcga tttcgccgag cggggtgacg tgtgcgattg
    13441 ggccgattgg ctcgaggcgc ggctggtcaa atagcgggac cacgccgcaa ttgccgcgaa
    13501 tcgcccctgg acacgtttgg gaaaactcgc gtaaactgtt gagcttccct gccccgctgc
    13561 tactttacgc gcaaaatcag aaaggagaga acgcatgtcg cagattcttc ggtcgtgggg
    13621 gtcccatggt taagttgatt gtccgcgaca aggaaagcat tcaggaggcc gttcgccgtt
    13681 ttcgcaagct ggtcgaacgg agcggcatta agaaggaaat gcgacgccgc gagtattacg
    13741 aaaagcccag tgaaatccgg cggcgggccc gtctgcgggc cgagcgccgc gctcgccgca
    13801 gcacgatgaa gccggtgggg atgtagcgcg gcggtgagtc ttacggcgtg aacgatctaa
    13861 agcgccttca gcagcgagtt gccccttcgc tacagtccgt gccgagtgaa aatctgtgcg
    13921 gcctgcgtag cgaggtgatt ctgggcaagg tgtgcttccg atacatctcg tgggtcgccg
    13981 tgggcggaac ggtacgcgca attgcggctt taactttcag tcgcgattgc cgagtacctc
    14041 aataccctgt gctgcccgct atcctgtttc ccgcgctcga tcggccaacc cgacggcgcg
    14101 gtccctccct gctgcaccag ctgcgccgcc gcgcggcgtt gatcgaggaa ggagcccccg
    14161 atgctcgtgt tgtcgaggca ccgtgacgaa agcatcatga ttggcgacga catcgttgtg
    14221 acgatcgtcg atatccgcgg cgacaaggtc cgcctgggga tcgaggcacc cactgccatt
    14281 cccgtccatc gccaggaagt ctatgaggcg atccagcggg agaatcgcaa ggccacgcag
    14341 ctgaagccgg aagacgcccg cgacgtgggc aagccgaatc cgccgcgcta aattgctccg
    14401 ccaggccgtc ttgcgtgcga aaccggacgc aattgcctcg caaatatctt tgaattttgc
    14461 ctgatccgcg cacgcttgtg ctcgaaattc cgcgttttcg ctggccgctg gcggcgagtt
    14521 ggaaaaaacc agagccgcgg cccctagcag gcatgccggc gcgccaccca caacctaaat
    14581 gagggtgagg ttcgacccag caggtttcct aaagcacgca accggacccg acgatgttgg
    14641 tgcagtagga accgcggcat ggcggctgcg ggattcaggg tcgtgcatct cgtcatggat
    14701 gtccggcatg acgcatgatc cctggctggc ccgtgccgat gcgggcgagc tgagcggaaa
    14761 acaagtcatc ggcgagcggg ctgcggaccc cttgtggcgg ctgcgacctg agcgaactcc
    14821 acccggcacg tgaagccggc cgcttgcagg cctgagcttc cgccccttca gcttacgaaa
    14881 ggttccctaa tgagtcgcat taacacgaac gtcagctcgc tgattgccca gaagatcttg
    14941 ggccgcacga acaaccagct gcaggaagcg ctgaaccggc tctcgaccgg tttgcggatc
    15001 aacgtcggca aggacgaccc ggcgggcttg atcgcgagcg agaacctccg ccgcgacatc
    15061 gccagcgcca acaaggcgat cagcaacacc gagcgggcca accagctcat cggcaccgcc
    15121 gacagcgccc tgggccagat cagcggcctg ctgattgata ttcgcgggct ggtcgtcgag
    15181 gccgccaaca ccggcgtgct cagcgacgag caactcgccg ccaaccagct ccaagtcgat
    15241 tcgtcgctgg aagcgatcga ccggatctcg caggtcacga cgttccaggg ccgcaagctg
    15301 ctcgacggca gcctcgactt ccagatcacc tacaccgccg gcggcgctac ggtcgaagac
    15361 ctgaacatcg accaggccaa tctcggcacg gccggctccg tggctgtgga catccagatc
    15421 tcggtcgcgg ccacgcaggc cctgatcacc aacgccgccc tgcccctgac cgacgacctg
    15481 gtggtcaaga tcgcaggcca gaagggctcg gaagtgttca cgtttgagtt gggtgcgacg
    15541 gtcacccaga ttgtggacgc cgtcaacctg gtcagcgacg ccacgggcgt gcaggccacg
    15601 gacaacggcg gcacgctcga gctggagtcc accgattacg gcagcgcagc cttcgtcgat
    15661 gtcgaagtcc tggaggaagg cgccctgggc acgttcgagg ccggcctgag cgcgacccgc
    15721 gaggtgggca tcgacatcgc ggccaccgtc aacggcgtca ctgccaacgg cgcgggcaac
    15781 acgctgagca tcaatacttc cacgctcgac ctgtcgttga ccgtggcgga cggcagtgcg
    15841 gtcgccgtcc agttcacgat cgacggcggc ggggccctgt tccagctcgg cccgcagatc
    15901 gtcagcaacc agcaggcccg catcggcatc ggcagcgtca acaccggcga gttgggcggc
    15961 gtcccgggcc gcctgtatca gctccgctcg ggcaacgacg ccgacctgac gaccgatgcc
    16021 cgcacggctt cgctgatcgt cgacgtggcg atcaacaagg tgaccggcct ccgcggccgc
    16081 ctgggcgcct tccagcgtgc gaccctggag accaacatcg cgtctcttca ggacaccgtc
    16141 acgaacctgg ccgaggccga aagctcgatc cgcgacgccg acttcgccgc cgagaccgcc
    16201 cgcctgacgc gggcccagat tctcgtgcag tccggcacct cggtgctcgc catcgccaac
    16261 cagaacccgc agaacgtgct cgccctcctg cgtggctaat tcagtcaagc ctccggtgtg
    16321 gaccgggcgt gaagcacgct cgccacgccg gacttaggat caccagccgc cggcccggaa
    16381 tcgctcccgg ccggcggtcg gcgtttccgg ttagtcggtt ggtcggacag tcggtcggtc
    16441 ggtgaatcgg taaagattgc ccaattcgcc ccgctgccga taccgaccac cgactgaccg
    16501 actgaccctc tgtctgccga taccgtctag ataagctcta acgcgcgctg tttagctagc
    16561 gaatggccgg catccaatcc agtacgggca tcatcaccgg catccccatc caggatacgg
    16621 tcgataagct catcgctctg caggcccagc cgcgagaccg cctgctggct cgccaaaagg
    16681 tgctgcaggc ggagcaagcc gccatcggtg acctgatggc cctgacgctg ggtgtgcagc
    16741 tcgccgtccg ccggctgaag aacctggatc tgttctcggc ccgcaacgcg accagcagca
    16801 atccggcatt gctgacggcc actgccagct ccgaggcgat tcccgggcag tatcagttcg
    16861 tcccgctccg cccggctcag tcgcatcacg cgctgtcgag cggcctggcc gcgcccgacg
    16921 cggctctcgg cggcggcacg ttcaccgtcc gctacggcgg tcaggtcgat cgcggaatca
    16981 gcctcgccga tctcaatagc ggcgcgggcg tcagccgcgg caagatcaaa atcaccgacc
    17041 gtagcggcgc atgggcgatc atcgacctgc ggtatgcgca aaccatcgac gacgtgctgg
    17101 ccgcgatcaa tgccagcgac gagatcgccg tccgtgctac ggccgccggc gaccatctgg
    17161 tcttgagcga cagctcgggc ggcacgggca acctgcgcgt gcaggaagtc ggcgacggca
    17221 ccacggcggc ggacctgggc ctggacggaa tcaacgtcgc tgccggcgag gccggcggca
    17281 ccgacctcg
//
LOCUS       DASRJB010000921         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2533416, whole genome shotgun sequence.
ACCESSION   DASRJB010000921 DASRJB010000000
VERSION     DASRJB010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533416"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFB80_24320"
     CDS             complement(<1..337)
                     /locus_tag="VFB80_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36987.1"
                     /translation="MAAVKASDKLTLRDRLSRLTFEQACKLLGPQGRKLIPLGAMWDY
                     QLQEDVFLGDDLFRLRLPRPDGSEPFAVTITLSAEARDRLHWQCDHCREACEHVGAAF
                     SLILEEKTAL"
     gene            508..1578
                     /locus_tag="VFB80_24325"
     CDS             508..1578
                     /locus_tag="VFB80_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZN36988.1"
                     /translation="MSPLSRRCLLAGSAALAGTALAPRTLPGQTPSDAARSSDLITNE
                     TQRAIDRGLTWLAKRQMVSGSGEGSFGHGGYPGGVAVASLSGLAFMCSGNPPGQGPLG
                     KNIDRCVKFVSSCVQDSGFISVPGSGQDNMYGHGFAMLFLSEVYGMSDRNEVDSTVGE
                     KLRRAVKLSATCQNDAGGWRYQPVKSDADLSITICQIMGLRAARDAGIHVPDEVRTKC
                     IQYVKNSQNADGGFRYQVSGGGSSFPLTGAGIVALYSAGIYDGEPIEKGLKWLMNHLP
                     NKGGGSSGYFFYGHYYAVQAMWHAGGEHWSKWYPAIRDTLLARQGGDGSWPDNEVCPE
                     FGTAMACIILQMPNNYLPIFSP"
     gene            1701..>2058
                     /locus_tag="VFB80_24330"
     CDS             1701..>2058
                     /locus_tag="VFB80_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36989.1"
                     /translation="MALSIGLGWLGSWLARVRHQRQIVARIKEAGGRVRYEHELNWVN
                     VGRNATHYEPKPPPGHWLVHRVVGEDALANVGEVTFDFRNPATDADLAQLDELPRLKW
                     LRIQGQGVSDASMATIA"
BASE COUNT          367 a          683 c          658 g          350 t
ORIGIN      
        1 ccagcgcggt tttctcttcc aggatcagcg aaaacgccgc tccgacgtgc tcgcacgcct
       61 cgcggcaatg gtcgcactgc cagtgcaacc gatcgcgagc ctccgccgac agggtaatcg
      121 tcaccgcgaa cggctcgctg ccatcgggtc gcggcagccg cagccggaac aggtcgtcgc
      181 cgagaaagac gtcctcctgc agctgataat cccacatcgc cccgagcggg atcagcttgc
      241 ggccctgcgg ccccagcagt ttgcaagcct gctcgaaggt cagccggctc agccgatcgc
      301 gcagcgtgag cttgtcgctc gccttgaccg ccgccatgcg ccgcactccg ttttgcgagg
      361 agcatccgta gccgaagatt tggaaactct taacaatgta ctgagaaccg ttgttgatgc
      421 aatcgcacag cagtggaaga ctatgcgggt catgccgagt ttggcctcgc ttctcaaaca
      481 aggtagactg gtccgggagg ctttcccatg tcgccactct ctcgccgctg cctgctagca
      541 ggatccgctg ctcttgcggg aactgcccta gcgccacgca ccttgccggg ccagacccct
      601 tcggacgccg cccgcagctc ggacctgatc acgaacgaga cgcagcgggc gatcgatcgc
      661 gggttgacgt ggctggccaa gcggcagatg gtctcggggt cgggcgaggg ctcgttcggc
      721 cacggcggct atcccggcgg cgtcgccgtc gcgagcctct cgggcctcgc gttcatgtgc
      781 agcggcaatc cgcccgggca ggggccgctg ggaaagaaca tcgaccgctg cgtcaagttc
      841 gtcagctcct gcgttcaaga cagcggcttc atcagcgtcc ccggctccgg ccaggacaat
      901 atgtacggcc acggcttcgc catgctgttc ctctccgagg tctacggcat gtcggaccgc
      961 aacgaagtgg acagcaccgt cggcgagaag ctgcggcggg ctgtcaagct ctcggccacc
     1021 tgccagaacg acgccggcgg ctggcggtat cagccggtga agagcgacgc cgacctgtcg
     1081 atcacgatct gccagatcat ggggctgcgg gcggcccgcg acgcgggcat tcacgtgccc
     1141 gatgaagtcc gcaccaagtg catccagtac gtcaagaaca gccagaacgc cgacggcggc
     1201 ttccgctacc aggtctcggg cggcggcagc agcttcccgc tcaccggggc gggcatcgtg
     1261 gccctgtaca gcgccggcat ctacgacggc gagccgatcg agaaggggct gaagtggctg
     1321 atgaaccacc tgcccaacaa gggcggcggc agctcgggct atttcttcta cgggcactat
     1381 tacgccgtgc aggcgatgtg gcacgccggc ggcgaacact ggtcgaagtg gtaccccgcc
     1441 atccgcgaca cgctgctggc ccgccaaggc ggcgacggct cctggcccga caacgaagtc
     1501 tgccccgagt tcggcaccgc gatggcctgc atcattctgc agatgccaaa caactacctg
     1561 ccgatcttct cgccataact gcccttagcc gggacaaacc tggtcccgga gcgccacgag
     1621 tggacgctgc cgcgcattcg actgctcctc ccaagcgccg ctggctgcgc ttcagtctgc
     1681 gcacgttgtt ggcggtttgt gtggcgctgt ccatcggttt ggggtggctg ggaagctggc
     1741 tcgcccgcgt ccgtcatcag cggcagatcg tggcccggat caaagaggcg ggcggccgcg
     1801 tacgatacga gcacgaattg aattgggtga acgtgggccg caacgccact cactatgagc
     1861 cgaagccgcc gccgggtcac tggctcgttc accgcgtcgt aggagaagac gcgctggcca
     1921 atgtcggtga ggtgaccttc gacttccgga atccggcgac agatgccgac ctagcccagc
     1981 tcgacgaatt gcctcgactg aagtggttga ggatccaagg ccagggagtg tctgacgcaa
     2041 gtatggccac gatcgcgc
//
LOCUS       DASRJB010000922         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1398949, whole genome shotgun sequence.
ACCESSION   DASRJB010000922 DASRJB010000000
VERSION     DASRJB010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398949"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1759)
                     /locus_tag="VFB80_24335"
     CDS             complement(128..1759)
                     /locus_tag="VFB80_24335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN36990.1"
                     /translation="MPRFTARPLPVLLAAAAALAAIWLLSPDDTRRALANSLELAAGA
                     TLIALPLGTLLAILIARFDLPGRAAAAAGLVVLLFLPLYVHLCGWEAAAGRIGWLTLN
                     YGSMAQPLLAGMRGAIVVHGLAAVPWVALIVGLGLSQIDAAQEEAGLLVLPPAGVLGR
                     ITLPQCLPFLVAAAIWTVVSTTSEMTVTNIFLMNATREKFERTYTEQFYSKFSLTADA
                     GEATLAAMPGVLGLIVILLLALWMVAQLGRRRNLSRPRGSVTFSAGAWRLLAAAVLWL
                     IVLALLAVPIAALVNKAGFVVLHDGEQRIRSWSLLAALRELAAAPLRFGVDFSWTLRV
                     AAAAASVATLLGVALAWPARRGGRRSWPAIAAVVLGLAIPGPLVGVALIQLFNHDLPP
                     RLAWGDGYKSWLLILYDNSPLAPILAQAIRALPLATLIAWHSLATLSDDVLGAAALDG
                     LSPWRVFWKIALRQRWRAIAAAWLAALAVAAGDLAWVHLVTPPGMDLIQRRVFGLVHS
                     GVEEQVAAIALVNIAFYSLLAAAILWLLRPRRSFR"
     gene            1946..>2633
                     /locus_tag="VFB80_24340"
     CDS             1946..>2633
                     /locus_tag="VFB80_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HZN36991.1"
                     /translation="MVSLLWLSLFGCSAESSAPSAAAPAAATAAAQPLDESRYARAAA
                     DAAPMPAALPAASAPVVESAPAGDGQGPGQGGDKFAYIEEGPFLSVKEAPLSTFSIDV
                     DTASYSKARQYLLENHTLPPPDAIRIEELVNYFEYDYAGPDGEHPFAVHVESAECPWQ
                     PKHRLVRFGIQGKKLDQQRPLSNLVFLIDVSGSMNQPNRLPLVQRALGMLVRQLGEND
                     RVAIVVYAGAA"
BASE COUNT          477 a          892 c          877 g          387 t
ORIGIN      
        1 gttgcgataa cctggcaacc atggcattcc tcgcactggc ttgtgtcatg attgtgtcaa
       61 gcagtgacac gatcatgtcc caagtgcagg tcaccggcaa gaggggggtg tccgaggcgc
      121 cttttgctta gcggaagctg cggcgagggc gcagcagcca gagaatcgcg gcggccagca
      181 atgaatagaa ggcgatgtta acgagggcaa tggccgcgac ctgttcctcg acgccggaat
      241 gcaccaggcc aaagacgcgg cgctggatca ggtccatccc cggcggcgtg accaggtgga
      301 cccaggccag gtcgcccgcc gccaccgcca gcgcagccag ccacgccgcg gcgatggccc
      361 gccagcgctg gcgcagcgcg atcttccaga acactcgcca cggggacagg ccatcgagtg
      421 cagcggcgcc gagcacgtca tcgctcagcg tggcgagcga gtgccaggcg atcagcgtcg
      481 ccagcggcag cgcgcggatc gcctgcgcga ggatcggcgc cagcggcgag ttgtcgtaca
      541 gaatcaacag ccaggacttg tagccgtccc cccaagccag gcgcggcggc aaatcgtggt
      601 taaagagctg gatgagcgcg acgccaacga gcggtccggg aatcgccaac ccgagtacga
      661 ccgctgcgat cgccggccag ctccgccggc cgccgcggcg ggccggccag gccagcgcaa
      721 ctcccagcag cgtggcgacg cttgccgcag ccgcggccac gcgcagcgtc caggaaaaat
      781 cgacgccgaa tcgcagcggc gccgcggcca attcgcggag cgccgccagc agcgaccagg
      841 agcggatccg ctgctcccca tcgtgcagca cgacgaaccc ggccttgttg accaaagcgg
      901 caattggcac ggccaggagc gcgaggacga tgagccatag caccgccgct gcgagcagcc
      961 gccacgcgcc ggccgaaaac gtcacgctgc cgcgcggccg cgacaggttt cgccgcctgc
     1021 ccagctgcgc caccatccac agcgccaaaa gcaggatgac gatcaggccg agcacgcctg
     1081 gcatagcggc cagcgttgct tcccctgcgt cggccgtgag cgaaaacttg ctgtagaact
     1141 gctccgtgta ggtccgctcg aacttttccc gcgtcgcgtt catcaggaag atgttcgtca
     1201 ccgtcatctc gctggtcgtg ctgacgacgg tccagatggc cgccgccacg aggaacggca
     1261 ggcactgcgg cagcgtgatt cgcccgagca cgccggccgg cggcagcacg aggagccccg
     1321 cttcttcctg tgccgcgtcg atttgcgaga ggcccaggcc gacgatcagc gccacccacg
     1381 gaaccgccgc caggccgtgc acgacgatcg ccccgcgcat cccggccaac agcggctggg
     1441 ccatcgagcc atagttcagc gtcagccagc cgatgcgtcc tgcggctgcc tcccacccgc
     1501 agaggtgcac gtagagcggc agaaagagca gcacgaccag ccccgcggcg gcggccgcgc
     1561 gtccgggcaa atcgaagcgg gcgatcaaga tcgccagcag cgtgcccagc ggcaaggcga
     1621 tcagcgtggc cccggctgct agctccaagg aatttgccag ggcccgccgc gtatcgtcgg
     1681 gcgagagcag ccagattgcc gccagcgcgg cggccgccgc gagcagaacc ggcagcggcc
     1741 gggcagtgaa tcgcggcaag gaaggcattt tcacagttta gttggtcccg cgggaatatt
     1801 cgccgcggcg gtgggctcat caaaacgaag gcgggagcca ttccgtcttt ccctgagagc
     1861 ctctgggccg ccgctcggct ggccgcgctc cggttttgcc ttcgatgtga ggacttcgct
     1921 catggtgcgc gcctggaata ttgttttggt atcgttgctc tggctgagtc tgtttggctg
     1981 ctcggccgaa tcgtctgcac cgagtgctgc agcgcccgct gcggcgaccg ccgcagcgca
     2041 gccgctggac gagagccggt acgcccgcgc ggccgccgat gctgcaccca tgccggcagc
     2101 actgcccgcc gccagtgctc ccgttgtgga aagtgccccg gccggtgacg gccaaggacc
     2161 cgggcaaggc ggcgacaagt ttgcctatat cgaagaaggt ccgtttctct cggtaaagga
     2221 agcgccgctt tcgacgtttt cgattgacgt cgataccgcg agctattcca aggcccgcca
     2281 gtacttgctg gagaatcaca cgctgccccc gccggatgcg atccgcatcg aagagctggt
     2341 caactacttt gagtacgact acgccggccc ggacggcgag catccctttg cggtgcacgt
     2401 cgagtcggcc gagtgcccgt ggcagccgaa acatcgcctg gtgcggtttg gcatccaggg
     2461 gaagaagctc gatcagcagc ggccgctcag caacctcgtg tttttgatcg acgtctccgg
     2521 ctcgatgaat cagcccaaca ggctgccgct ggtgcagcgg gccctcggca tgctcgtgcg
     2581 gcagctgggc gaaaacgacc gcgtggcaat cgtggtctat gccggagcgg cgg
//
LOCUS       DASRJB010000923         5710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2800740, whole genome shotgun sequence.
ACCESSION   DASRJB010000923 DASRJB010000000
VERSION     DASRJB010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800740"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFB80_24345"
     CDS             <1..237
                     /locus_tag="VFB80_24345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36992.1"
                     /translation="PVGIDGAYQAWPRTSRLPRPGRIAVVIGEPIPPARLEEFSDEDL
                     LAELEQRILSAHALARELRVNPSQSTGASKSAAP"
     gene            complement(165..935)
                     /gene="bioD"
                     /locus_tag="VFB80_24350"
     CDS             complement(165..935)
                     /gene="bioD"
                     /locus_tag="VFB80_24350"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZN36993.1"
                     /translation="MALTISSLLAPQPRNAARRPRPPATAGLFVVGTDTAVGKTYVAS
                     RIAAALARSGLKVGVYKPAASGCRRVGKLLLSDDAVNLWEAAGRPGELKHVCPQRFAA
                     PLAPHLAAKEERKEIDERLLRRGIEYWQRRSDVVVVEGAGGLMSPIGERDYVADLAEA
                     FGYPLIVVAPNRIGAINSTLLTLMVAASRPRPLPIAGIVLNDVLPRDRGDPAIHSNRV
                     ELELRCVPPVLCQLGHGAADFDAPVDWLGLTRSSRASA"
     gene            1136..1570
                     /locus_tag="VFB80_24355"
     CDS             1136..1570
                     /locus_tag="VFB80_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36994.1"
                     /translation="MVRTACLAAVAALLLAAPQTASAYWPWGGNFAGYGYGYGYGAWD
                     ASYFNGGIYAAPLPYYSVYPPVYYSHQITARHYGASPYAWYSGQEPITFVPHPAPPMG
                     PMPVMVENPYVAKPMPAPAARADASEPMPQKIDNPFVASSSR"
     gene            1665..2150
                     /locus_tag="VFB80_24360"
     CDS             1665..2150
                     /locus_tag="VFB80_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN36995.1"
                     /translation="MSRVLGMVGIAAALAGCQAQNPYSAFGPNRVPPPGMQSPAPYYP
                     AGATAAPAAAPASAGAATTGRLSVSAKSSAPSSPIASRAQTSGEDAIRIVENPAPAAR
                     TAAAGGTRSAGAAPAAAAPSSSPPAVPTREKSSSIDRKDAEVAQAAYETAAAPGQWKS
                     R"
     gene            complement(2152..4947)
                     /locus_tag="VFB80_24365"
     CDS             complement(2152..4947)
                     /locus_tag="VFB80_24365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN36996.1"
                     /translation="MSAPSPEVTVDAFLDALSKSGLLTAEAMAAAQAAAAKHADPKAA
                     ARELIKSGTLTRWQATQLLHGFHQLVIGKFKLLEQLGAGEMGRVYLAEHAQIGRRHTL
                     KVLARRHMANPELVKRFLADAASACSLDHRNISHVFDVTQEGDRCYVVMEYVEGENLD
                     KLIQRRGKLPVREALEYVRQATEGLAHAHDSGIIHGDLKPSNLLLEGGGTLKILDFGQ
                     SRLFTAPAGGADESREVPTLAARMFQSPEVRGTADRAPDARSDVYSLGSVLCFLLSGK
                     PALDAGEAAKELQAAGAQAEVVEWCQRLMAEDPAQRPASMAGLLSELGGLARALTALP
                     PKPKAESRPEPTAESRPVENRPVERPAPKVKKPPVARPLEEPALPVAKTIDEAPASGD
                     AEESEPQAAAPFAGIAVQSPGRGKKPPVKAAPQVAVAIAPAAEKPAALRPAKSSQAPQ
                     LIIAAVCGVLLLGGGGFLIAKFALGGNDKPVAAAPAKSPPVTAVKKSDDAKAAEANPV
                     ETNPAESNPAETNPVEANPTVAAPVQTPPLPAATAATEKTKNEPPAAKTTEPTTPEPT
                     PEPKPAPPTKAAAPPAKTEPKAKAKPDEEPKPKPEANPFAGFAKSITLPPLAEGMTGE
                     ALAPATLGPCQVEGEAIVHASLKGGETAIRGSKLKFDLQPKNGTAPRDWEFHLIGGEA
                     PTVIATLAAKEGNLVFQWTEAAAKQVLHTKQLCNGAIELACGANKHLVALREPAMGAP
                     LAIEFEKPQSAKWTIENLPDPKSIQIEITKVDGLPKLKQEPIGSAAIGEDLQIWTGPD
                     ADSLFLCLRIDTSPQARGLQVASTPQLRLPAMAKPAKYSKKEVTAARQTTNDQLASLT
                     NQLTLLSKQKKTQQTDAMKGKIQSTIDEMNKGAAVIQKLFDFVASMQGIGTIQFRVFH
                     QADDAQIDLLITGGDAK"
     gene            5111..5641
                     /locus_tag="VFB80_24370"
     CDS             5111..5641
                     /locus_tag="VFB80_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN36997.1"
                     /translation="MKLFFLMLTAVMLLASIASAAEPLKVGDEAPDFTMVGSDGKTYK
                     LSDFKGKQAVVVAWFPRAFTGGCTKECTSFKNDGAKMREYEVAYFTASNDPAEQNKKF
                     AESLSVDYPILSDPDSSVATKYGVYNPDRKAALRWTFYIGADGKVLFIDKAVKTDAHG
                     ADVATKLGELGVAKKK"
BASE COUNT          859 a         1926 c         1892 g         1033 t
ORIGIN      
        1 cccgtcggca tcgacggcgc gtaccaggcc tggccgcgga ccagccggct gccgcggccc
       61 gggcggatcg cggtcgtcat cggcgagccg attccacccg cgcggctcga agagttcagc
      121 gacgaagacc tgctggcgga actggagcag cgaatcctca gcgctcacgc gctggcgcgc
      181 gagctgcgcg tcaatccgag ccaatcgacc ggcgcgtcga aatcggccgc gccgtgaccg
      241 agctggcaca gcacgggcgg cacgcagcgg agctccagct ccacgcggtt gctgtggatc
      301 gccggatcgc cgcgatcgcg gggcaacacg tcgttcagca cgatccccgc aatcggcagc
      361 ggccgcgggc gggaagccgc aaccatcagc gtgagcagcg tgctgttgat cgcgccgatg
      421 cggttggggg ccacgacgat cagcggatag ccgaacgcct cggccaggtc ggcgacgtag
      481 tcgcgctcgc cgatcggcga catcagcccg cccgcccctt cgaccacgac cacgtcgctc
      541 cgccgctgcc agtattcgat cccccgccgc agcagccgct cgtcgatctc cttgcgttcc
      601 tccttggcgg ccaggtgcgg ggccagcggc gcggcgaaac gctgcgggca gacgtgcttc
      661 agctcgcccg gccgtccagc ggcctcccac aggttcacgg cgtcgtctga aaggagcagc
      721 ttgccgacgc ggcggcagcc gctggccgcc ggtttgtaga cgccgacctt gagaccggac
      781 cgcgcgagcg ccgcggcaat tcgggacgca acgtaggtct tgccgacggc cgtgtcggtg
      841 ccgacgacga acagccccgc cgtggccggc ggacgcggcc gccgggccgc atttcgcggc
      901 tgcggagcga gcaggctgga gattgtcagg gccatgtttc tcacgctacc aacagccgga
      961 gggcggcgac agcggacgtg gcgctaagat aactgaacat cgctcgggcg gacttgacat
     1021 ccggcggccg gcgctgaatt gcgcgcggca gcgcattgaa acggcgcggg gccgtaatta
     1081 cggtggagcg aagtggagtg tgttttcgcc gcctcttgct ttgtgagatt gccacatggt
     1141 tcgcaccgct tgtctggccg ccgttgcggc cctgcttctc gccgcaccgc agaccgccag
     1201 tgcgtactgg ccgtggggcg gcaacttcgc tggttacggc tacggatatg gctacggcgc
     1261 ctgggacgcc agctatttta acggcgggat ctacgccgcg ccgctgccgt attactccgt
     1321 ctatccgccg gtgtattaca gccatcagat caccgcgcgg cactacggcg ctagccccta
     1381 tgcctggtac tccggccagg agccgatcac gttcgtgccg caccccgcgc cgccgatggg
     1441 tccgatgccg gtgatggttg agaaccccta cgtcgccaag ccgatgccgg cccctgccgc
     1501 gcgggctgac gccagcgagc cgatgccgca aaagatcgac aatccgttcg ttgccagcag
     1561 cagccgctag cggccggtct gctcttggtt cactagcgcc tcgccggtac agtccgcgcg
     1621 aggcgttttc gttttcttgc gcgtcttggc agcgagggcg attcatgtcg cgggtgcttg
     1681 gaatggtcgg catcgccgcg gccctggccg gctgccaggc gcagaacccc tattccgcgt
     1741 tcgggccgaa ccgcgtgccg ccgccgggca tgcaatcgcc cgcgccgtat tatcccgccg
     1801 gcgcgaccgc agcgcccgcg gctgccccag ccagcgccgg agcggcgaca acgggccgct
     1861 tgtcggtctc ggccaaatcg tcggccccgt catcgccaat cgccagccgc gcacagacga
     1921 gcggcgagga cgcgatccgg atcgtcgaaa atcccgcgcc cgcggctcgc acggccgccg
     1981 ccggcggcac gcgctccgcg ggggctgcac ccgcagctgc cgctccgtcg agttcgccgc
     2041 cggcagtgcc gacgcgcgag aaatcgtcga gcatcgaccg gaaagatgcc gaggtggcgc
     2101 aggcagcgta cgaaacggcc gccgcgccgg gccagtggaa gtcgcgctaa gctacttcgc
     2161 gtcgccgccg gtgatcagca ggtcgatctg ggcgtcatcc gcctggtgaa agacacggaa
     2221 ctggatcgtg ccgatgcctt gcatcgaagc gacaaaatcg aacagcttct gaattactgc
     2281 cgcgccctta ttcatctcgt cgatcgtcga ttgaatcttg cccttcatgg cgtcggtttg
     2341 ctgggttttc ttttgctttg acagcagggt cagctggttg gtgagcgacg ccagctggtc
     2401 gttcgtcgtc tgccgggccg ccgtcacttc cttcttgctg tacttcgcgg gcttggccat
     2461 tgccggcagc cgcagctgcg gcgtggaggc gacttgcagg ccgcgggcct gcgggctggt
     2521 atctattcgc aggcacaaga acagcgaatc ggcgtccggt ccagtccaaa tctgcaggtc
     2581 ctcgccgatc gcggccgagc caatcggctc ctgcttgagc ttgggcaagc cgtccacctt
     2641 ggtgatctcg atctggatgc tcttggggtc gggcaggttc tcgatcgtcc acttggccga
     2701 ctggggcttt tcgaattcga tggccagcgg cgcgcccatc gccggctctc gcagcgcaac
     2761 gagatgctta ttcgcgccgc aggccagttc gatcgcgcca ttacagagct gcttggtgtg
     2821 cagcacctgc ttcgcggcgg cttccgtcca ctgaaacacc aggttgcctt ccttggcggc
     2881 cagcgtggca attaccgtgg gtgcctcgcc gccgataagg tggaattccc agtcgcgcgg
     2941 cgcggttccg tttttgggct gcaggtcgaa cttcagtttg ctgccgcgaa tcgccgtttc
     3001 gccgcccttg agactggcgt ggacgatcgc ttccccctcg acctggcacg gcccaagcgt
     3061 agcgggcgct agcgcctcgc ctgtcatccc ttctgcgagc ggcggcaagg tgatcgactt
     3121 cgcgaatccc gcgaatggat tggcctcggg cttgggcttt ggctcttcgt ccggcttcgc
     3181 cttcgccttc ggctcagtct tcgcaggcgg cgcggccgcc ttagtcggcg gagcgggctt
     3241 gggctccggc gtcggctcgg gagttgtagg ctccgtcgtc ttggcggcag gcggctcgtt
     3301 cttggttttt tcggtggccg cggtagccgc cggcaaagga ggcgtctgga cgggagcggc
     3361 gaccgtggga ttcgcctcca cgggattcgt ctcggctgga ttcgattccg caggattcgt
     3421 ttccaccgga ttcgcttcgg ccgccttcgc gtcgtccgat ttcttgacag ccgtgactgg
     3481 cggagacttc gcgggcgctg cggcgaccgg cttgtcgttg ccgcccaagg caaacttcgc
     3541 aatcagaaag cctccgccgc cgagcaacaa tacgccgcag accgcagcga tgatcagctg
     3601 cggcgcttgg ctcgacttcg cgggccggag ggcagccggc ttctcggcag caggcgcgat
     3661 ggccaccgcc acctgcggcg cggccttgac gggcggtttc ttgccgcgac ctggagactg
     3721 aacagcaatg ccggcaaatg gagcggctgc ttggggttcg gactcctcgg catctccgga
     3781 cgccggcgct tcgtcaatgg tcttggcaac tggcaacgcc ggctcctcga gcgggcgggc
     3841 gaccggcggc ttcttcacct tgggcgcggg ccgctccact gggcgattct cgactgggcg
     3901 actttcggcg gtgggctcgg gcctggactc cgccttcggc ttaggcggca gggcagtcag
     3961 cgctcgggcc aggccgccca gttcactcag caagccggcc atcgacgcgg gccgctgcgc
     4021 cggatcctcg gccatcagcc gctggcacca ctccacgact tccgcttgcg caccggccgc
     4081 ctgtaattct ttagcggcct cgccggcatc cagggctggc ttgcctgaaa gcagaaagca
     4141 gagcacgctg ccgagcgagt agacgtcgct gcgggcatcg ggtgcgcgat cggccgtgcc
     4201 gcgcacttcg ggcgactgaa acatccgcgc ggccagcgtc ggcacttccc gcgactcatc
     4261 cgctccgccc gccggcgcgg taaacagccg cgattgcccg aagtccagaa tttttagcgt
     4321 gcctccgcct tcgagcagca gattggacgg cttcaggtcg ccgtgaatga tgccgctgtc
     4381 gtgcgcgtga gccagcccct ccgtcgcctg ccggacgtat tccagcgcct cgcgaacggg
     4441 cagcttgccg cgccgctgga tcagcttgtc gaggttctcg ccttcgacgt attccatcac
     4501 gacgtagcag cggtcgcctt cctgggtgac gtcgaagacg tggctgatgt tacggtggtc
     4561 gaggctgcac gcgctggccg catcggcgag gaaccgcttc acgagttccg gattggccat
     4621 gtgccgccgg gccagcacct tcagcgtgtg gcggcggccg atctgagcgt gctcggccag
     4681 atacacgcgg cccatctcgc ccgcgccgag ctgttcgagc agcttgaact tgccgatcac
     4741 cagctggtga aacccgtgca gcagctgggt cgcctgccac ctcgtcagcg tgccggattt
     4801 gatcagctcg cgggcggcgg ccttggggtc ggcgtgtttc gcggctgctg cctgagccgc
     4861 ggccattgct tccgccgtca gcagcccgct cttgctgagg gcgtccaaaa acgcatcgac
     4921 agtgacctcg ggcgacggcg ctgacatgaa gcccccggaa aattgctgct cgaccttaga
     4981 ttctgaccat aacgcctgcc gattgccaga cctttcagtc cggcgcgttg ccgggcggct
     5041 cggcggcgtg ttaggctgcc cgctgctgcg tttggctccc ccgcccacct acttagctgg
     5101 aggtttcccc atgaagctgt tcttcctgat gctgaccgcc gtgatgctgc tggcttcgat
     5161 cgcgtcggcc gccgagccgc tcaaagtcgg cgacgaggcc cccgatttca cgatggtcgg
     5221 cagcgacggc aagacctata agctgtccga cttcaaaggc aagcaggccg tcgtggtcgc
     5281 ctggttcccg cgggccttca cgggcggttg cacgaaggaa tgcacgtcgt tcaagaacga
     5341 tggtgcgaag atgcgggaat atgaagtcgc ctacttcacg gccagcaacg acccggccga
     5401 gcagaacaag aagttcgccg agtcgctcag cgtcgattat ccgatcctca gcgatccgga
     5461 cagcagcgtg gccacgaaat acggcgtgta caaccccgat cgcaaggcgg ccctgcggtg
     5521 gacgttctat atcggggccg acggcaaggt cctgttcatc gacaaggccg tgaagaccga
     5581 cgcccacggc gccgacgtcg ccacgaagct cggcgaactg ggggtcgcga agaagaagta
     5641 gcatctgcct gaccgataat gcctgggtgt tcccgctgcc gacggcggga actcgctttt
     5701 cgctgtacag
//
LOCUS       DASRJB010000924        13980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1966959, whole genome shotgun sequence.
ACCESSION   DASRJB010000924 DASRJB010000000
VERSION     DASRJB010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966959"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFB80_24375"
     CDS             <1..260
                     /locus_tag="VFB80_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZN36998.1"
                     /translation="SITCFEPEARGQLAAGMAFHKYYFDLPGNPQRPAKNVTMAQPYV
                     RLTQSLDASGQPQTARMEETRIWQKIGGQWKHVHFHRSAGT"
     gene            complement(642..1592)
                     /gene="epmA"
                     /locus_tag="VFB80_24380"
     CDS             complement(642..1592)
                     /gene="epmA"
                     /locus_tag="VFB80_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HZN36999.1"
                     /translation="MAADFLPTASLPMLRRRAELLKQVRRFFEDRGFLEVETPLLSHD
                     TVVDRHLDPLGATLPSDPRQPLAGPRLWLQTSPEFAMKRLLAAGVTAIYQVTKAFRGG
                     EAGPLHNPEFTIVEWYRVGDDYAAGMQLLSDLAESLLDLGPAERLTYREAFRQFAGVD
                     PFADPMQIVLEDLPPRADGDLVLDYLLTTRVEPHLGQNRPTILHDYPASQAALAQLRP
                     GQPPVAERFELYVRGIELANGYHELLDPALLRDRNRANNELRAAGGKYTLPAESRLLV
                     AMDHGLPACSGCALGFDRLVMVATSAKSIQEVMAFPIERA"
     gene            complement(1605..2192)
                     /locus_tag="VFB80_24385"
     CDS             complement(1605..2192)
                     /locus_tag="VFB80_24385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZN37000.1"
                     /translation="MSLRSICVFCGSSVGGRAEYAAAASAMGRLLAERGIRIVYGAGN
                     VGLMGVLADAALKAGGEVVGVIPQMLVDRELAHRGLSELRIVGSMHERKALMAELSDA
                     FIALPGGLGTLEELCEVLTWAQLGIHHKPVGCLNVQDYFDGLFQLLDHASSEQFVRPQ
                     HRGLLLAADDPEQLLQLLQARQPPIAEKRFDREVT"
     gene            complement(2189..3094)
                     /locus_tag="VFB80_24390"
     CDS             complement(2189..3094)
                     /locus_tag="VFB80_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37001.1"
                     /translation="MLKRATILSAAVILSIASLAHAQTALRWKLQSGQKLAISITQTT
                     ESIVVYSGKKTATQIDLSMELTWRVISADDGKFRIEQSLDRLSVALDSPPAGRVQYDT
                     AQQAKPTGSAKEIAAALDPLLATKLEITMTDRGEIVDARPVQGSPPAATDPASSGPVP
                     KESIQQLLRQPLAILPEKPVNQGGTWNSTSELKTALGMATQTTTYRYTGPGEVDGHKL
                     EQVAISAELKVAPSDKAKIKLKEHQQSGVVLFDATAGRVASTEQKQKLVTERPYRETT
                     IVVTLDSKQKTKVVAVGDSPAAAQP"
     gene            complement(3119..3808)
                     /locus_tag="VFB80_24395"
     CDS             complement(3119..3808)
                     /locus_tag="VFB80_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN37002.1"
                     /translation="MPTEITWLGHATWRLKTDQHTLLVDPFLDDSPTAPFKAKDARAD
                     YILVSHGHYDHIADAAQIANRTGATVISNYEITTWLAKHHGVKNTIGGNLGGGVNLPL
                     GRVKLTLAFHSSSLPDGGNGGNPCGFLISLTAGPKVYFACDTALFSDMQQIGAGGLDL
                     AVLPIGDLFTMGPDDALEAVQLLKPKQVVPSHYNTWPPIAQDPDRWAERVRSETKTTP
                     IVVQPGQPFTI"
     gene            complement(3818..5194)
                     /locus_tag="VFB80_24400"
     CDS             complement(3818..5194)
                     /locus_tag="VFB80_24400"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HZN37003.1"
                     /translation="MRPAWLAILLLLPVHAATQARDVDPRQYGIDLPAGAVLAGENQA
                     VTTTDLAGEGVVGRIHVRVGDGAVVLLPDGQLVARKAGQFAATDRKFEPVTKEALAER
                     LAAEFPGFQVKMTNHYLYVYDTSEEFAFGTGRILETMLPGVKGWADLQRLPAQNPALP
                     LVAVMFQKEEDFQKYRRMPTGVVAYYHTLSNRVFMYEQSRLARVRPDLAVQQAISTVA
                     HEGAHQILHNIGVQQRLSIWPMWLSEGLAEFFAPTTMTDKLKWKGAGQINDLRMFELE
                     QYLKSKAADDPTGEMVEYTVAAGRLTSTGYATAWSLTHYLAKNRRPEFTALLRECSQL
                     GPFEGATEIVPPGIVRANREQFSKVFGDDFKAVESRLVLHLKKQPYTDPFRNSPHFVA
                     TLLTSEGRRQQRSALTFHSPQLATNWLNDSLAKFTAEQRAAAQTAVRPFPNRQQAEAY
                     AAQWLAGR"
     gene            5309..7795
                     /locus_tag="VFB80_24405"
     CDS             5309..7795
                     /locus_tag="VFB80_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZN37004.1"
                     /translation="MPRVPLLLLAIAALVVAGVTPALAADRRVVLLPSTIALSSREAR
                     QTLVVQWQAGEQFQEQATPADVEFSTSNDKVVKVEEGVATAVGNGEATITARVGNQAA
                     TASVAVKNFDEPFAWSFRNHVESVLSKQGCNAGACHGARAGQKGFRLTLFGFDVDADY
                     TYLTRQAVGRRIVPTDPGRSLILTKPTGLLPHKGGVRLEPGSLEYRVLSEWIASGVPG
                     PKAGDPQIERVEVLPKYSLQSLGKSQQLVVLAHFSDGHVEDATRWAKYTSVQGTVAAV
                     DERGKVSITGAGEAAVKVWYLNHNAMAFLTVPFPNEVRPKVFAQAPRRNFIDELVLAK
                     LQALRVPPSPRCTDAEFLRRAYLDTIGTLPTADEAREFLDDTAADKRDRLIDELLGRP
                     EFVDYWTYRWSDLLLLSGERLRPKSIETYYAWIRERVARNQPWDEFVKELVTASGSTH
                     ENGAANFFALHQDPEGMAETVAQAFMGLSINCAKCHNHPLEKWTNDQYYSFANMFSRV
                     RAKGWGGDFRNGDGNRVVYTDTQGELIQPSRGVPQPPAPLDGQPLAFEATADRRLAVA
                     EWLASSDNPYFTRAIVNRVWANFFGVGLVEAVDDLRLTNPASNEELLNAAARHLIAHR
                     YNLKELMRTILESETYQRTSQPLPENQADERFYSRYYPRRLKAEVLLDAISQVTAVPT
                     DFKAGTPDSRKPGPALPKIKRALHLPDGFVESYFLSAFGKPDRLITCECERSNEPSMT
                     QVLHLYNGDTVNQKLAKPGSAAEKALAEVDNKQIVESLYLAALSRLPTEKESASLVET
                     LSAAPVEERRQAIEDLHWSVLTSREFLFNH"
     gene            7799..10573
                     /locus_tag="VFB80_24410"
     CDS             7799..10573
                     /locus_tag="VFB80_24410"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HZN37005.1"
                     /translation="MRTLLSLVIVACAAPALCSADAAPDFGKDVAPIFTKYCTGCHND
                     SDREGKLSLESYASLLKGGAKGGVVTPGQPELSRLVRMLTGAAEPKMPPKGEEPPKPG
                     EIEVLKAWIAAGAKGPSGTAADPTLLVTPQVKLLAPPKLPINAVALSPDGTLLAVARH
                     GEVELLTFPGREAVEKFGGLRGSVNGLAFSRDGNRVVAASGEPGLFGEATIFPADGTD
                     SLPVSFRGHKDSLYAAALSPDGTILATGGYDSTIKLWNVQEVKELRTLEGHNGAVFEL
                     AFRPDGKVLASASGDRTVKLWNVATGERLDTLKESQKELYTLAFSPDGSRLAGGGVDN
                     RIRVWQITEGAKEGSNPLLFSTFAHETPVLRLVWSADGRTIATCGEDRLVKLWNAEAM
                     TIRQTLPKQSDWASGLAITKNGDSLAVGRIDGSLEVYPLAAAAAGDERVLTPLAEVPP
                     AVDYGSQPPVDMLPKVAEAEPNELPERATRLSAAGIATGKIALADHDLFRFAAKAGDQ
                     WIIETKAARAGSPLDTKIEVLDDAGRPVPRILLRAVRDSLIEFRGMTSEQRGVRLANW
                     EEMLLNEYVYLSGDVIKLFQQRRGPDADGQFYPENGNRFAFFDTTPRAHALGEPAYIV
                     VPYAIGTELPNNGLPVFTLNYENDDDAQRKLGKDSRLTFVAPADGEYLVRVADVRGFG
                     GDNFSYELIVRRPQPDFKVTFTPKDLTINAASGKPFTVKAERTDNFMGPIRVDVAGLP
                     PGLSVTSPVVIQEGLYEATGLISAAADAAAPTAEQVKEIKITATADVCGTERSQAVSG
                     LGTLKVADKPKVIVHLESIVEQASPLAQSEQARTLVLRPGGTITARLRVERNGFDDRI
                     AFDVANLPHGVIVDDIGLSGVLIPEKQTERTIFLRAEPWVSEQTRPFFATAQVDGNQC
                     SLPLVLQVVK"
     gene            complement(10574..11323)
                     /locus_tag="VFB80_24415"
     CDS             complement(10574..11323)
                     /locus_tag="VFB80_24415"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HZN37006.1"
                     /translation="MFPDLTSDERLRYQWQLWVRDFGEEGQRKLKAASVLVSRIGGVG
                     GSAALQLAAAGVGRLMIAHAGNLRLDDLNRQLLMTTDCIGKPRVESAARRLRELNPLV
                     EVETIAENVTESNAATLVGRCDVVVGAAPLFTERLWLNREAVRQGKPLVDCAMYDLDG
                     RLTTILPGRSPCLACLYPEPPPNWKREFPVFSAVASTVGSLAAMEVIKVIAGFGAPLA
                     GKLLTFDLREMTFQTVAITRRADCAVCGGRT"
     gene            complement(11431..11703)
                     /locus_tag="VFB80_24420"
     CDS             complement(11431..11703)
                     /locus_tag="VFB80_24420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZN37007.1"
                     /translation="MKVRVQFTAQLRTALGRAAEEVELPEGSSVAELLRQLVNRFDGA
                     APHLLKPTGEPQSSLLIVINEAVATTQSVHLRAGDVVTLLPPIAGG"
     gene            complement(11700..12395)
                     /locus_tag="VFB80_24425"
     CDS             complement(11700..12395)
                     /locus_tag="VFB80_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN37008.1"
                     /translation="MIELDDISIRSGSFELRRVSLSIPAGSYAVLMGATGQGKTTILE
                     AICGLRTVTGGRVLLGGVDVTRRKPADRGVGYVPQDLALFPMMTVRGHLEFALRVRRW
                     PAAAIGPRVTELAQELGIEPLLARDVRRLSGGEAQRVALGRALSFRPQVLLMDEPLNA
                     LDEATRDRLCELLGKVQSASHLTTLHVTHSRAEARALADRLIVLEGGQLYERPVADLE
                     QLPDEMLEGTPRR"
     gene            complement(12392..13246)
                     /locus_tag="VFB80_24430"
     CDS             complement(12392..13246)
                     /locus_tag="VFB80_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN37009.1"
                     /translation="MSDQPAAPAAHRAGSDVPFYVVMGGLGGSYVVLIVLLLAADLAF
                     TSPGHFAAALAKPEIQYAIRLTLVSCTISALLSIWVATPLGYLLSRFQFRGRWLIDTI
                     IDIPIVLPPLVIGLSLLILFHLPVAGTNLETFLQEHVSIGRRPFRVTYAQPAVVLAQF
                     AVACAFAVQTMRVTFDQIDPRAEQVALTLGCRRSQAFLQVALPQAWRGMIAATTVAWA
                     RSLGEFGPILVFAGATRMKTEVLSTTVFLELSVGQLEAAVAVSLLMVIAAMTVLGIVR
                     LLGARAAT"
     gene            complement(13239..>13980)
                     /locus_tag="VFB80_24435"
     CDS             complement(13239..>13980)
                     /locus_tag="VFB80_24435"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN37010.1"
                     /translation="FGFQPAAGDAWNEQPEITLYAGSMLRPAIEKTITEFEQREGVRV
                     TRVYNGCGILVAQMKAGEVPDAYFACDSEFMKQVQPLFRNPLNVSQNELVILVHKGNP
                     LGIASLKDLAKPNIRVGIGHEKQCAMGWLTQRTFDETGYKSELMKNVVVQTPTGDMLV
                     NQMRSGSLDAAVAYISNAAGAGDALDAIRIQGIPCSVATQPFAVAKDSAHQQLVQRLL
                     AAIRAESSHRRFTSEGFQWLAEGPPAHE"
BASE COUNT         2457 a         4534 c         4629 g         2360 t
ORIGIN      
        1 cgtcgatcac ctgcttcgag cccgaggccc gcggccagct cgccgccgga atggcgtttc
       61 acaagtatta cttcgatctg cccgggaacc cgcagaggcc ggccaagaac gtcacgatgg
      121 cccagccgta cgtccgcctg acgcaatcgc tcgacgccag cggccagccg caaaccgccc
      181 gcatggaaga gacgcgcatc tggcagaaga tcggcggcca atggaagcac gtgcacttcc
      241 accgctcggc ggggacgtag gttcgaccgg aattatggac cggacaattc ggtcaacttg
      301 attccgcacc ttgtcagagc gcgaaactcg gcatcttcgc gttcgtattg ggcttgaagt
      361 cgcgtgattt caggatggag tcggcgtttg agatgagcgc gatccttggc tcggagcgct
      421 ggagcgtaat ccacattttg ttccgttagc ttcaggtgct gaactgcagt cagtgggtcg
      481 tgaatgccca gtgagacata tcgtttcaat tcggtctcga catctgcatg aatcctatat
      541 cgcattcgga cttcgtcgca ccactgccaa aatggaagac cgacaccgac cattgttgtc
      601 acgagaaaca aggtgcgcag gcgataacgc atctgcgtaa tctaagcccg ctcgatcggg
      661 aacgccatca cttcctggat cgactttgcg ctggttgcga ccatcacgag gcgatcgaag
      721 cccagggcgc agccggagca ggcgggcaag ccgtgatcca tggcgaccag cagccggctc
      781 tcggcgggca gagtgtactt gccgccggca gcccgcagct cgttgttcgc gcggttacgg
      841 tcgcggagca gagccgggtc gaggagttcg tgatagccgt tggccagctc gatgccgcga
      901 acatacagct caaaccgctc ggcgacgggc ggctggcccg ggcggagctg ggcgagggcg
      961 gcctggctgg cgggatagtc gtgaaggatc gtgggtcgat tctggcccag atgcggctcg
     1021 acgcgcgtcg tcagcagata gtcgagaacg agatcgccgt cggcccgggg cggcaggtcc
     1081 tccaggacaa tttgcatcgg atcggcaaac ggatcgacgc cggcgaactg gcgaaaggcc
     1141 tctcggtaag taagccgctc ggccgggccc aggtccagca gcgattcggc caggtcggac
     1201 agcagctgca tgccggccgc atagtcgtct cccacgcgat accactcgac gatcgtgaat
     1261 tcgggattgt gcagcgggcc cgcctcgccg ccgcggaaag ccttggtcac ctggtagatg
     1321 gccgtgacgc ccgccgccag cagccgcttc atcgcgaatt ccggcgaggt ctggagccac
     1381 agccgcggcc cggccagcgg ctgccgcgga tcgctaggga gcgttgcgcc cagcgggtcg
     1441 aggtggcgat cgacgaccgt gtcgtgcgag aggagcggcg tttcgacttc cagaaacccg
     1501 cggtcttcga agaaccggcg gacctgcttc agcagctccg cgcggcggcg cagcatagga
     1561 agcgatgcgg tgggcaggaa gtcggcggcc atggtcgaaa ctgcctacgt gacctcgcgg
     1621 tcgaatcgtt tttccgcgat tggcggctga cgcgcttgga gcaactggag cagctgctcc
     1681 ggatcgtcgg cggccaggag caagccgcga tgttgcggac ggacaaactg ctcgctggag
     1741 gcatgatcga gcagctggaa cagtccatcg aaatagtcct gcacgttcag gcagccgacg
     1801 ggcttgtggt gaatgccgag ctgggcccag gtgaggactt cgcacaattc ttcgagcgtg
     1861 cccagcccgc cgggcagggc gatgaaggcg tcggacagct cggccatgag ggcctttcgc
     1921 tcgtgcatcg agccgacgat tcgcagctca gaaaggccgc ggtgagccag ctcgcgatcg
     1981 acgagcatct gcggaatgac gcccacgacc tcgccgcccg ccttcaaagc cgcgtcggcc
     2041 agcacgccca tcaggccgac attgccggcg ccatagacaa tgcggatgcc gcgctcggcc
     2101 agcaggcggc ccatcgcgct cgccgccgcg gcatattcag cccgcccgcc gacgctggag
     2161 ccgcagaaga cacagatgga gcggaggctc atggctgcgc ggcggcgggg gaatcgccga
     2221 ctgccacgac tttcgtcttc tgcttgctgt caagcgtgac gacgatcgtc gtttcgcgat
     2281 acggccgctc ggtgaccagc ttctgctttt gctcggtgct ggcgacgcgg cccgcggtgg
     2341 catcgaacag cacgacgccg gattgctgat gctccttgag cttgattttc gccttgtcgc
     2401 tcggcgcgac cttcagctcc gccgagatgg cgacctgttc cagcttgtgg ccgtccactt
     2461 cgcctgggcc ggtatagcga taggtcgtcg tctgcgtggc cattcccaga gcggtcttta
     2521 gttcgctggt cgagttccag gtgcctccct ggttgaccgg cttctcaggc agaatcgcca
     2581 gcggctgccg cagcagttgc tggatcgact ccttgggcac cggcccgcta gaggccggat
     2641 ctgtcgcggc tggcggcgag ccttggaccg gcctggcatc cacaatctcg ccgcggtcgg
     2701 tcatcgtgat ttccagcttc gtcgccagaa gcggatccag tgccgccgca atttccttgg
     2761 ccgagccggt gggcttggcc tgctgcgccg tgtcgtactg cacacggccg gccggcggcg
     2821 aatcgagggc tacgctcagg cgatcgagcg attgctcgat gcggaacttg ccgtcgtcgg
     2881 cagagatcac gcgccacgtc aattccatcg acaggtcgat ctgcgtggcc gtcttcttgc
     2941 cggagtacac gacgatggat tctgtcgtct gggtgatcga gatcgccagc ttctgtccgc
     3001 tctgcagctt ccagcggagc gcggtctgag cgtgggccag ggacgcgatc gaaaggatga
     3061 ccgccgccga gagaatcgtt gcacgcttca gcatttggac gtctcctcac cccaacggtc
     3121 agatcgtgaa gggctggccg ggctggacga cgatcggcgt cgtcttggtc tcggagcgaa
     3181 cgcgctcggc ccagcgatcc gggtcctgcg cgatgggcgg ccaggtgttg tagtgcgacg
     3241 gtacgacttg cttcggcttg agcagctgga ctgcttccaa ggcgtcgtcc ggccccatcg
     3301 taaacaagtc gccgatcggc agcacggcca ggtcgagacc gcccgcgccg atctgctgca
     3361 tgtcgctgaa caaggcggtg tcgcaggcga aatagacctt cggcccggcc gtgagcgaaa
     3421 tcagaaagcc gcaaggattg ccgccattgc cgccgtcggg cagcgacgag ctgtggaagg
     3481 ccagcgtcag cttcacgcgg cccagcggca ggttgacgcc gccgcccagg ttgccgccga
     3541 tcgtgttctt gacgccgtgg tgcttcgcga gccaggtcgt gatctcgtag ttcgagatca
     3601 ccgtggcccc cgtgcggttg gcgatttggg ccgcgtcggc aatgtggtcg tagtggccgt
     3661 gcgacacgag gatatagtcg gcgcgggcgt ccttggcctt gaagggcgcc gtcggcgagt
     3721 cgtcgagaaa tggatcgacg agcagcgtgt gctggtcggt cttcaagcgc caggtcgcgt
     3781 ggccaagcca ggtgatttcg gtgggcatgc gatcctccta tcgcccggcc agccactgcg
     3841 ccgcataggc ctcggcctgc tgacggttcg gaaacggccg caccgccgtc tgagccgcag
     3901 cccgctgctc ggccgtgaat ttcgccagtg aatcgttcag ccagttggtg gccagctgcg
     3961 gcgagtgaaa cgttagcgcg ctccgctgct gccgccggcc ctcgctggtg agtagcgtcg
     4021 caacaaagtg cggcgagttc cggaacggat cggtgtaggg ctgttttttg aggtgcagca
     4081 ccagccgcga ttcgacagct ttgaaatcgt cgccaaacac cttgctgaat tgctcacggt
     4141 tggcgcggac gatgcccggc ggcacgatct cagtggctcc ttcgaacggt ccgagttggc
     4201 tgcactcgcg cagcagggcg gtgaactccg ggcggcggtt cttggccagg taatgcgtca
     4261 gggaccaggc ggtggcatag ccggtagagg tcagccgccc cgcggcaacg gtgtattcga
     4321 ccatttcgcc ggtcggatcg tcggcagcct tgctcttgag atactgctcg agctcgaaca
     4381 tccgcaggtc gttgatctgg ccggcccctt tccacttcag cttgtcggtc atggtggtgg
     4441 gggcgaagaa ctcggccagt ccttcgctga gccacatggg ccagatgctc agccgttgct
     4501 gcacgccgat gttgtgcaga atctggtggg cgccctcgtg ggccaccgtc gaaatggcct
     4561 gctgcacggc caggtccggc cgcacacggg ccagccgcga ctgctcgtac atgaagacgc
     4621 ggttgctgag cgtgtggtaa taggccacga cgccggtcgg catgcggcga tacttttgaa
     4681 agtcttcctc tttctggaac attacggcga cgagcggcaa ggccggattt tgggccggca
     4741 gccgctgcaa gtccgcccaa cctttgacgc cgggcagcat cgtctccagg atgcggccgg
     4801 tgccgaaggc aaattcttcg ctggtgtcgt agacgtacag atagtggttg gtcatcttga
     4861 cctggaagcc gggaaactcg gcggcgaggc gctcggccag cgcttctttc gtgaccggtt
     4921 cgaatttgcg gtccgtcgcc gcgaactggc cggccttgcg ggccaccaat tgcccgtccg
     4981 gcaacagcac gacggccccg tcgccgaccc gcacgtgaat tcgcccgacg acgccttccc
     5041 ctgcgaggtc cgttgtcgtt accgcctgat tctcgccggc gaggacagca cccgcgggca
     5101 ggtcgatccc gtactgccgc ggatcgacgt cgcgggcctg cgtcgcggcg tggacgggga
     5161 gcaagagtag aatggcgagc caggccgggc gcatggtttt atcgtagctg ccgcgcggcg
     5221 gggcggaaag gtcgcgagcg gcaagcggca gcccaggcat gttaagatac gtggaccctc
     5281 cttcagatcg aacctctgca atacagccat gccgagagtc cctctcctcc tgttggccat
     5341 tgcggcgctt gtagtggcgg gagtaacgcc cgccttggct gccgaccgcc gcgtggtgct
     5401 gttgcccagt accattgcgc tctcgtcgcg cgaggcccgg cagacgctgg tcgtgcagtg
     5461 gcaggcgggc gagcagtttc aggagcaggc cacgccagcc gacgtggaat tcagcaccag
     5521 caacgacaag gtcgtgaagg tcgaagaagg cgtcgccact gcggtgggca atggcgaggc
     5581 gaccatcacc gcccgcgtcg gcaatcaagc ggcgacggcg agcgtcgccg tgaagaactt
     5641 cgatgagccg ttcgcctgga gctttcgcaa ccatgtcgaa tcggtactgt ccaagcaggg
     5701 ctgcaatgca ggtgcgtgcc acggcgctcg ggccgggcag aagggctttc ggctgacgct
     5761 gtttggcttc gacgttgatg ccgattacac ctatctcacg cggcaggccg tcggccgccg
     5821 catcgtaccg accgatcctg gccgcagcct gattcttacc aagccgacgg gcctgttgcc
     5881 gcataagggc ggcgtgcggc tggagccggg ctcgctggaa taccgcgtgc tgtccgagtg
     5941 gatcgctagc ggcgtgccag gtcccaaggc cggcgatcca caaatcgagc gcgtggaagt
     6001 gctgcccaag tactcactcc agtcattggg caaatcgcag cagctcgtcg tgctggcgca
     6061 cttcagcgac gggcacgtcg aggacgcgac gcggtgggcc aagtacacga gcgtccaagg
     6121 gacggtcgcc gcagtcgatg aacgcggcaa ggtgtcgatc accggcgcag gcgaagcggc
     6181 ggtcaaggtc tggtacctca atcacaacgc gatggcgttc ctgaccgtgc cgtttccgaa
     6241 cgaggtgcgg cccaaggtct ttgctcaagc accgcggcgg aacttcatcg acgaactggt
     6301 gctggccaag ctccaggccc tccgcgtgcc gccgtcgccg cgctgcaccg acgccgaatt
     6361 cctccgccgg gcgtatctcg acacgatcgg tacgctgccg acagccgacg aggcccgtga
     6421 atttctggat gacacggccg ccgacaagcg cgatcggttg atcgacgagc tgctcggccg
     6481 gccggagttt gtcgattatt ggacctatcg ctggtccgat ttgctgctgc tctcgggcga
     6541 gcggctgcgg cccaagtcga tcgagaccta ttacgcttgg attcgcgagc gggtcgccag
     6601 gaatcagccc tgggacgagt tcgtcaagga attggtaact gccagcggca gcacgcacga
     6661 gaacggcgcg gccaactttt tcgccctgca tcaggacccc gagggcatgg ccgaaacggt
     6721 ggcccaggcc tttatggggt tgtcgatcaa ctgcgccaag tgccacaacc atccgctcga
     6781 gaaatggacc aacgaccagt attacagctt cgccaacatg ttctcgcgcg tgcgggccaa
     6841 aggctggggc ggcgatttcc gcaacggcga cggcaatcgc gtggtctata ccgacacgca
     6901 gggcgaattg atccagccca gccgcggcgt gccgcagccg ccggctccgc tcgacgggca
     6961 gccgctggcg tttgaggcga cggccgaccg ccgcctggcc gtcgccgagt ggctcgcctc
     7021 ctcagataat ccgtacttca cgcgggccat cgtcaatcgc gtctgggcca actttttcgg
     7081 cgtcggcctg gtagaggcgg tcgatgatct gcggctgacc aacccggcca gcaatgagga
     7141 actgctgaac gccgcggcgc gacatctgat cgcccatcgc tacaacctca aagagctgat
     7201 gcgaacgatc ttggagtccg agacctatca gcggacgagc cagccgctcc cggagaacca
     7261 ggccgacgaa cggttctact cgcgctacta tccgcggcgc ttgaaggccg aggtgctgct
     7321 cgacgccatt tcccaggtca cggccgtccc gaccgacttc aaggccggca cgcccgacag
     7381 ccgcaagccc ggcccggccc tgcccaagat taagcgggcc ctgcacctgc ccgacggctt
     7441 cgtcgaatcg tactttctga gcgccttcgg caagcccgac cggctgatca cctgcgagtg
     7501 cgagcggagc aacgagccga gcatgacgca ggtgctgcac ctctataacg gcgacacggt
     7561 gaaccagaag ctcgccaaac ccggtagcgc agccgagaag gccttggctg aagtggacaa
     7621 caagcagatt gtcgagtcgc tgtacctcgc ggccctgtcg cgtctaccga ccgaaaagga
     7681 atcagcttcg ttggtcgaga ctctgtccgc tgcgcccgtc gaggagcgac gtcaagccat
     7741 cgaagacttg cactggagcg tccttacgag ccgggagttt ctgttcaacc actgaatcat
     7801 gcgaacccta ctctctctcg tcatcgttgc ctgcgccgcg ccggcactct gctcagctga
     7861 tgccgcgccc gattttggca aggacgtcgc gccgatcttc acgaagtact gcacgggctg
     7921 ccacaacgac agcgaccgcg aggggaagct gtcgctggaa tcgtacgcgt cgctgctcaa
     7981 gggcggggcg aaaggcggcg tcgtgacgcc gggccagccg gagctcagca ggctggtgcg
     8041 catgctgaca ggcgcggccg agcccaagat gccgcccaag ggcgaggaac cgcctaagcc
     8101 gggggaaatc gaagtgctca aggcctggat tgcggcaggc gcgaaaggcc ccagcggtac
     8161 ggcggccgat ccaacgctgc tcgtgacgcc gcaggtgaaa ttgctcgccc caccgaagct
     8221 gccgatcaat gccgtcgcgc tgtcgcccga cggcacgctg ctggccgtcg cgcggcacgg
     8281 tgaagtcgag ctactgacct ttccggggcg ggaggcagtg gagaagttcg gcgggctccg
     8341 cggcagcgtc aatggcctgg cgttctctcg cgacggcaac cgtgtcgtcg cggcgtcggg
     8401 cgagccggga ctcttcggcg aagcgacaat cttccctgct gacggcacgg acagcttgcc
     8461 ggtcagtttt cgcggccaca aggacagtct gtatgccgcc gcgctcagtc ccgatgggac
     8521 gatcctggcg acgggcggat acgacagcac gatcaagcta tggaacgtcc aggaagtcaa
     8581 ggagctgcgg acgctcgagg gccacaacgg cgcggtcttt gagctggcgt tccggccgga
     8641 cggcaaggtg ctggccagcg ccagcggcga ccgcacggtc aagctttgga acgtcgccac
     8701 cggcgagcga ttggacacgc tcaaggagtc gcagaaagag ctgtacacgc tggccttcag
     8761 ccccgacggc tcgcggctgg ccggcggcgg cgtcgataac cgcatccgcg tgtggcagat
     8821 cacggagggc gccaaggaag gcagcaatcc tctcctgttt tcgaccttcg cacacgagac
     8881 gcccgtcctg cggctggtct ggtcggccga cggccggacg atcgccacct gcggcgagga
     8941 tcggctcgtc aaactctgga acgccgaggc gatgaccatt cggcagacgc tgcccaagca
     9001 gagcgactgg gccagcgggc tggcgatcac caaaaatggc gactcgctgg ccgtcggccg
     9061 catcgatgga agcctcgagg tttatccgct ggccgcggct gccgcgggcg acgagcgggt
     9121 actgacaccg ctagccgaag tgccgcccgc agtcgattac ggttcgcagc cgccagtgga
     9181 catgctgccg aaagtcgcgg aggccgagcc gaacgagctg cccgagcgag cgacgcggct
     9241 ctccgctgcc ggcattgcga cgggcaagat cgcgctggcg gaccacgacc tgttccgctt
     9301 cgcggccaag gccggcgacc agtggatcat cgaaaccaaa gccgcccgcg cgggctcgcc
     9361 gctcgatacc aagattgaag tgctggacga tgccggccgg ccggtcccgc ggatactgtt
     9421 gcgggccgtc cgcgacagcc tgatcgagtt ccgcgggatg accagcgagc agcgcggcgt
     9481 gcggctggcg aattgggaag agatgctgct caacgagtac gtctatctca gcggcgacgt
     9541 gatcaagctc ttccagcagc gccgcggccc cgacgccgac gggcagttct atcccgaaaa
     9601 cggcaaccgt tttgccttct tcgacaccac gccgcgggcc cacgcgctgg gcgagccggc
     9661 ctacatcgtc gtgccgtacg cgatcgggac ggagctgccc aacaacggcc tgcccgtctt
     9721 cacgctcaac tacgaaaacg acgacgacgc ccagcggaag ctcggcaagg actcgcggct
     9781 gacgttcgtc gccccggccg atggtgagta tctggtgcgg gtcgccgacg tgcggggctt
     9841 cggcggcgac aacttctcct acgagctgat cgttcggcgg ccgcagccgg acttcaaggt
     9901 caccttcacg cccaaggacc tgacgatcaa cgccgccagc ggcaagccgt tcacggtcaa
     9961 ggcggagcgg accgacaact tcatggggcc gatccgcgtg gacgtggcgg gcctgccgcc
    10021 ggggctgtcg gtcacatcgc cggtggtgat tcaggagggg ctctatgagg cgacgggcct
    10081 gatcagcgcc gcggccgacg ccgccgcgcc gactgcggag caagtcaagg aaatcaagat
    10141 cacggccacg gccgacgttt gcggcacgga gcggtcgcaa gccgtcagcg gcctcggcac
    10201 gctcaaggtc gctgacaagc ccaaagtgat cgtccatctg gaatcgatcg tagagcaagc
    10261 gtccccgctt gctcaatcgg aacaagcgag gacgcttgtt ctacggcccg gcggcacgat
    10321 cacggcccga ctccgcgtcg agcggaacgg attcgatgac cgaatcgcct tcgacgtggc
    10381 caacctgccg cacggcgtca tcgtcgatga catcggtctc tccggcgtgc tgattccaga
    10441 gaaacaaacc gagcggacca tctttctccg cgccgagccc tgggtgagcg aacaaacccg
    10501 gccgttcttt gccaccgccc aggtggacgg caatcagtgc tcgctgcccc tcgtgctgca
    10561 agtcgtcaaa tgactatgtc cgcccgccgc agacagcgca gtcggctcgg cgggtgatcg
    10621 cgacggtctg aaacgtcatt tcccgcaggt cgaacgtcaa cagcttgccc gccagcggcg
    10681 cgccgaagcc ggcgatgacc ttgatgacct ccattgcggc cagcgagccg acggtggatg
    10741 ccaccgcgct gaacaccggg aactcgcgct tccagttggg tggcggctcg ggatagaggc
    10801 aagccaggca gggcgagcgg ccgggcagga tcgtcgttag ccggccgtcg agatcgtaca
    10861 tcgcgcaatc gacgagcggc tttccctgac ggactgcctc gcggttgagc cacagccgtt
    10921 cggtgaagag cggtgcagcg ccgacgacga cgtcgcaacg gccgaccagc gtggcggcgt
    10981 tggactcggt gacgttctcg gcgatcgttt caacttcgac gagtgggttc agctcgcgca
    11041 gccgccgcgc ggccgattca acccgcggct tgccgatgca atcggtcgtc atcaggagct
    11101 ggcgatttag gtcgtccagc cgcaagttgc cggcgtgagc gatcatcagc cggcccacgc
    11161 cggccgcggc cagctgcagc gccgccgagc cgccgacgcc gccgatccgc gagaccagca
    11221 cgctcgccgc cttcagcttc cgctgaccct cttcgccgaa gtcgcgcacc cagagctgcc
    11281 actggtagcg gaggcgctcg tcggacgtga ggtcggggaa catgagctgg gagtcggttg
    11341 gtcggtcagt cggtggtcag cggcgccctt gctctatgct gagtactggg tacgcagtct
    11401 tattcaagcg ctggaaaagc tcctttgctc ctagcctccc gcaattggag gcaacagcgt
    11461 cacgacatcg ccggcgcgaa gatgcacgga ctgagtggtt gcgaccgcct cattgatcac
    11521 gatcaacagg ctgctctgcg gctctcccgt gggcttgagc agatgcgggg ctgcgccatc
    11581 gaaacggttc accaattgcc ggagcagctc cgccacgcta ctcccttccg gcagctcgac
    11641 ctcttccgcc gctctgccta gggccgtgcg aagctgggcg gtgaattgca cgcggacttt
    11701 catcgcctgg gggtcccttc cagcatttcg tcgggcaatt gctccaggtc ggctaccggc
    11761 cgctcgtaca gctggccgcc ctctaggaca atcagccgat cggccagcgc gcgggcctcg
    11821 gcccggctgt gggtcacgtg tagcgtcgtg aggtggctcg cgctctgcac cttgcctagc
    11881 agctcgcaca ggcggtcgcg agtcgcttcg tccagcgcgt tgagcggctc atccatcagg
    11941 agcacttgcg gccggaacga cagcgcgcgg ccgagggcca cgcgctgcgc ttcgccgccg
    12001 ctgagccttc ggacgtctcg ggccaagagc ggctcgatgc ccagctcctg ggccagctca
    12061 gtcacgcgcg ggccgatcgc cgcggccggc cagcgccgca cgcgaagggc aaattccagg
    12121 tgcccgcgga cggtcatcat cggaaataga gccaaatctt gcggaacgta gcccacgccg
    12181 cgatcggccg gcttgcgacg cgtgacatcg actccgccca acagcacgcg gccgcctgtc
    12241 accgtccgca agccgcagat cgcctccagg atcgtcgtct tcccctggcc cgtcgccccc
    12301 atcagtacgg cgtacgagcc ggccgggatc gacaggctca cgcgccgcag ctcgaacgag
    12361 ccggagcgga tcgaaatgtc gtccagctcg atcatgtcgc ggccctcgcg cccaagagcc
    12421 gcacgatgcc cagcaccgtc atcgcggcga tgaccatcag cagcgatacg gcaacggccg
    12481 cttctagctg cccgacgctc agctccagga agacggttgt cgagagcact tcggtcttca
    12541 tccgcgtcgc gccggcgaaa acgaggatcg ggccgaactc gcccagcgaa cgggcccagg
    12601 cgaccgtcgt cgccgcgatc atcccccgcc aggcctgcgg cagggcgacc tgcaaaaacg
    12661 cctggctgcg gcggcagccg agcgtgaggg cgacctgctc ggcccgcgga tcgatctggt
    12721 cgaaggtcac ccgcatcgtc tgcacggcga acgcgcaggc cacggcgaac tgggccagca
    12781 ccacggccgg ctgggcgtaa gtcacgcgga atggccggcg gccaatgctc acgtgctcct
    12841 gcaggaacgt ctccaggttc gttccggcga ccggcaggtg aaagaggatc agcaggctga
    12901 gcccgatcac cagcggcggc aggacgatcg gaatgtcgat gatcgtgtcg atcaaccacc
    12961 gtcctcgaaa ctgaaatcgc gacagcaaat atcccagcgg cgtagccacc caaatcgaaa
    13021 gcagcgccga gatcgtgcat gagaccagcg tcagacggat cgcgtactgg atctccggct
    13081 tggccagcgc ggcggcaaag tgccccggcg acgtgaacgc gaggtcggcc gccagcagca
    13141 gcacgatcag gacaacgtag ctcccgccga gcccgcccat cacgacataa aagggcacat
    13201 ccgagccggc gcggtgggcg gctggggcgg cgggctggtc actcatgtgc cggcggcccc
    13261 tcggcaagcc actggaatcc ctcgctcgtg aaacgccggt gcgacgactc ggcccgaatc
    13321 gccgccagca gccgctggac gagctgctgg tgggccgagt ccttggccac ggcgaagggt
    13381 tgcgtggcga cggagcaggg aatgccctga atgcggatcg cgtcgagggc gtcgcccgcc
    13441 ccggcagcgt tgctgatata ggcgacggcg gcgtcgagcg aaccggagcg catttggttg
    13501 acgagcatgt cgccggtggg cgtctgcacg acgacattct tcatcagctc gctcttgtag
    13561 ccggtctcgt cgaaggtacg ctgcgtcagc cagcccatcg cgcactgctt ctcgtggccg
    13621 atgccgacgc ggatattcgg tttggccagg tccttcagcg aggcgatccc cagcgggttg
    13681 cccttgtgca ccagaatcac gagctcgttc tgggagacgt tcagcggatt gcgaaacagc
    13741 ggctgcacct gcttcatgaa ctccgagtcg caggcgaaat aggcgtcggg gacttcgccg
    13801 gccttcatct gggcgaccag aatgccgcag ccgttgtaga ctcgcgtgac gcggacgcct
    13861 tcgcgctgct cgaactcggt gatcgtcttc tcgatcgccg gccgcagcat cgagccggca
    13921 tagagcgtaa tctctggctg ctcattccag gcgtcgcccg ccgccggctg aaatccgaac
//
LOCUS       DASRJB010000925         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2246696, whole genome shotgun sequence.
ACCESSION   DASRJB010000925 DASRJB010000000
VERSION     DASRJB010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246696"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFB80_24440"
     CDS             complement(<1..258)
                     /locus_tag="VFB80_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN37011.1"
                     /translation="MSVIEIERLSKSYRVYQKQDGLWGAMRGLWRREYREVRAVREVS
                     LRVEKGEFVAFLGPNGAGKTTTLKLLSGVIYPTSGTARVLGY"
     gene            382..3504
                     /locus_tag="VFB80_24445"
     CDS             382..3504
                     /locus_tag="VFB80_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37012.1"
                     /translation="MKTSSPAVLASLTVFVTMLAFDRPSVAAEVGVRIRFGLTDKEPQ
                     EWDGTVSVKPGQVAAISGWRFELTDRANGTEGWTASTRPAAVARTNAQKAKAKAKQAA
                     NKAKTPEQKAKAAANQKAKAAAGAALADNGVLLTLANVDETSVVSVKTAQGDFTITLA
                     EIPYGKVVEKLDGAVEVERIAAARQVSTDRKTDDDYPSAAAAPDGTVYVVCASYTPGI
                     DRNERARTWQTAPDDLQFLADAPGGDQLLVRVVKGGASGEPLAVTESGADIYKSAVAV
                     AGDGTAWIFWSQNKNYKPFPNNPTANFDIYARPLKAGKLGETVRISESSESDVWPVAA
                     TDPAGNVWIAWQGARQGSFKILARRQTSGGWSPEMTISTHSRNCWAPAIATTKSGGGK
                     VAIAWDTYDKGDYDVFVREFDAAAKGGEARPVVNSADYEARPAIAYDREGALWVAWEQ
                     SGPTWGKDWGALVQRKGIGLYKDRQIGLAVLKGGQWMEPPQLVAQALPGVLPRRRINN
                     VRVPAIEPGAESRTAGQEAEAARNNVHNNIARLLCDAGGRIWLLARARQNDFRAQIGS
                     VWMTHATYYDGNAWVGPILVPHTDNLLYNSPAAVPLPGGGLLVAHSSDHRQDRHVNQR
                     GANAQGNLVGEDPFDNDLYLSRLEIGGDAKPATLVVSTTPPDPSAVPSDATVKERAEI
                     ERIRGYRAQIGGKNLQIVRGEFHRHTEISGDGGNDGPLEDMWRYAIDVAAMDWLGCGD
                     HDNGGHREYPWWLTQKTTDAFHLPGVFEPPFTYERSVRYPEGHRNVVFTRRGIRTLPR
                     LPITDRDNEIHAPDTQLMYKYLKLFDGVCAVHTSATSMGTDWRDNDPAVEPMVEIYQG
                     ARQNYERPGAPRSPTADDAIGGWEPKGFVNLALLKGYKFSFQSSSDHGSTHISYALVY
                     AEKNSRDAILAAMKQRHTYAATDNIIADYRCTSGGKEHLMGDAFTSTVPPTVKVKLAG
                     TAPFAKVTLVKDDVEIPLEAPQQAELELTWTDPKPEPGKESYYYIRGEQSDGELVWVS
                     PMWITYQPAR"
BASE COUNT          748 a         1262 c         1198 g          547 t
ORIGIN      
        1 atagcccagc acgcgggccg tgccgctcgt cggatagatc acgcccgaaa ggagcttgag
       61 cgtcgtggtc ttgcccgctc cgttgggtcc gagaaacgcg acgaactcgc ccttctcgac
      121 tcggaggctg acttcccgga ccgcccgcac ctcgcgatac tcgcggcgcc acaggccgcg
      181 catcgccccc cacagcccgt cctgcttctg gtacacgcgg tacgacttgg agagccgctc
      241 gatctcgatg acggacatgc acccagtata gcccaatgtc gaatgtcgaa attcaaacaa
      301 cgaataccag cctggcttga gatcgcttgc agacgatgta gaatcgcgcc gaacgaaatc
      361 tctcccacac ttgagcgacc gatgaaaacc tcctcgcctg ccgtgctggc ctctttgacc
      421 gtgtttgtga cgatgctggc gttcgatcga ccatcggtcg ctgccgaagt gggcgtgcga
      481 atccgcttcg gcttgaccga caaggagccg caggagtggg acggcacggt gagcgtcaag
      541 ccgggccagg tggcagcgat cagcggctgg cgattcgagc tgaccgaccg ggccaatggc
      601 accgagggct ggaccgcctc gacgcggccc gcggcagtgg cgcggacgaa tgctcagaag
      661 gcaaaggcca aggccaagca agcggcgaac aaggccaaaa cgcccgagca gaaagccaaa
      721 gcggccgcga accagaaggc caaggccgcc gcgggcgcgg ctcttgccga caacggcgtc
      781 ttgctgacgc tggcgaatgt tgatgagaca tcggttgtct cggtgaagac tgcgcagggc
      841 gacttcacga tcacgctggc cgagattccg tatggcaagg tcgtcgagaa gctcgacggc
      901 gcggtcgaag tcgagcggat cgccgcggcc cggcaggttt ccaccgaccg caagaccgac
      961 gacgactatc ccagcgccgc cgccgcaccg gacggcacgg tctacgtcgt ctgcgccagc
     1021 tacacgcccg gcatcgaccg caacgagcgg gcgcggacct ggcagacggc tccagacgat
     1081 ttgcagttcc tcgccgacgc gccgggcggc gatcaactgt tggtgcgagt cgtgaagggc
     1141 ggcgccagcg gcgagccgct ggccgtgacc gaaagcggcg cggacatcta taagagcgcg
     1201 gtcgccgtcg caggcgacgg cacggcctgg atcttctggt cgcagaacaa gaactacaaa
     1261 ccgttcccca acaaccccac ggccaatttc gacatctacg cccgcccgct gaaggccggc
     1321 aagctgggcg aaaccgtccg catttcggaa tcgtccgaaa gcgacgtctg gcccgtggca
     1381 gcaactgacc cagccggcaa cgtctggatc gcctggcagg gcgctcgcca aggcagcttc
     1441 aagattctcg cccgccgcca gacgtccggc ggctggtcgc cggagatgac gatcagcacg
     1501 cactcgcgaa attgctgggc cccggcaatt gcgacgacga agtccggcgg cggcaaggtc
     1561 gcgatcgcct gggacaccta tgacaagggc gactatgacg tgttcgtgcg tgagttcgac
     1621 gccgcagcca agggcggcga ggcccggccc gtcgtcaact cggccgatta tgaagcccgc
     1681 ccggcgatcg cctatgaccg cgaaggcgcg ctgtgggtcg cctgggagca gagcggtccg
     1741 acctggggca aagactgggg cgcgctcgtg cagcgaaagg gcattggcct atacaaggac
     1801 cggcagatcg ggctcgccgt gctgaaaggc ggccagtgga tggagccgcc gcagctcgtg
     1861 gcccaggccc tgcccggcgt gctgccgcgg cggaggatca acaacgtccg agtcccggcg
     1921 atcgagcccg gtgcggaatc gcggaccgcc ggccaggaag ccgaggctgc ccgcaacaac
     1981 gtgcacaaca acatcgcccg cctgctctgc gacgccggcg gccggatttg gctcctggcc
     2041 cgcgcgcggc agaacgattt ccgggcccag atcggctcgg tctggatgac gcacgcgacc
     2101 tactacgacg gcaacgcctg ggtcggcccg atcctcgtgc cgcacaccga caacctgctc
     2161 tacaacagcc ccgcggctgt cccgctgccg ggcggcggac tgctcgtcgc ccactcgagc
     2221 gaccaccggc aggaccggca cgtcaaccag cgcggcgcga atgcccaggg gaatctggtc
     2281 ggcgaagatc cgttcgacaa cgacctgtac ctcagccggc tggagatcgg cggcgacgcc
     2341 aagccggcga ccctcgtcgt gtcgacgacg ccccccgatc cgagcgccgt gccgagcgac
     2401 gctacagtga aggagcgggc cgagatcgag cggattcgcg gctaccgcgc tcaaatcggc
     2461 gggaagaacc tgcaaattgt ccgcggcgaa ttccaccggc acaccgagat cagcggcgac
     2521 ggcggcaacg acggcccgct ggaggacatg tggcgatacg cgatcgacgt ggccgcgatg
     2581 gattggctcg gctgcggcga ccacgacaac ggcggccacc gcgaatatcc ctggtggctg
     2641 acgcagaaga cgaccgacgc cttccacctt cccggagtgt tcgagccgcc cttcacttac
     2701 gaacggagcg tgcgctaccc cgaggggcac cgcaacgtcg tcttcacccg ccgcggcatc
     2761 cgcactctcc cgcggctgcc gatcaccgac cgcgacaacg agatccacgc gcccgatacg
     2821 cagctgatgt acaagtacct gaagctgttt gacggcgtct gcgccgtgca caccagcgcc
     2881 accagcatgg gcaccgactg gcgcgacaac gacccggctg tcgagccgat ggtcgagatc
     2941 tatcagggag cgcggcaaaa ctacgaacgg cccggcgctc cgcgcagccc gacggccgac
     3001 gacgccatcg gcggctggga gcccaagggc ttcgtcaacc tggccctgct caagggctac
     3061 aagttctcgt tccagtcgag cagcgaccac ggctcgacgc acatcagcta cgccctggtc
     3121 tatgccgaga agaactcgcg cgacgcgatc ctggccgcga tgaagcagcg gcacacctat
     3181 gcggcgaccg acaacatcat cgccgactat cgctgcacct ccggcggcaa ggagcacctg
     3241 atgggcgacg ctttcacgag cacggttccc ccaaccgtga aggtcaagct cgccggcacc
     3301 gcgccgttcg cgaaagtgac gctggtgaag gacgatgtcg agattccgct cgaagctccg
     3361 caacaggccg agctggagct gacctggacc gatcccaagc ccgagcccgg caaggagagc
     3421 tactactaca tccgcggcga acagtccgac ggcgagctcg tctgggtctc gccgatgtgg
     3481 attacgtatc agccggcgag gtagcggggc aaagttctca caacgctgaa caggccctaa
     3541 ccagggggtc tttgctagcg cgtctcgcat tgatgcgtct gctgcaaatc acttccctaa
     3601 cacgacttaa gttaaattgc cagagctcaa attcagatta gcgttagaag cgtcaaaagg
     3661 gctctgaaca ggtctcgcaa aatcgcatta gctctgcctg caccgcacca gcgtcgcgac
     3721 ggaattgtgg gtttctgaag ttcgactcgg actgt
//
LOCUS       DASRJB010000926         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1400232, whole genome shotgun sequence.
ACCESSION   DASRJB010000926 DASRJB010000000
VERSION     DASRJB010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400232"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFB80_24450"
     CDS             complement(<1..894)
                     /locus_tag="VFB80_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZN37013.1"
                     /translation="MTSPLHAEFYADQAVQVAAPIVENVRLARDTYRVRFHAPQIARR
                     ITPGQFVMLRIADLNDPLIGRPLALYDTALSAAGELEAVDVVYLVKGKLTSRLWQLQP
                     GQRLDVWGPLGNGFCHHSPLTTHHSPGPIPTEHLIMVAGGIGQTPFLALAQEALHKKR
                     YGDPPRDAPRALRVTFCYGARTADYLAGVEDFQRLGIDVRLATDDGSRGHRGFVSDLL
                     KQVLAEHHGRPRIVCCGPEPMMEAVAKIAHAAQVPCQVSLETPMACGIGICFTCVAKV
                     KQPAGGWDYKRTCVEGPVFDAE"
     gene            complement(891..2198)
                     /locus_tag="VFB80_24455"
     CDS             complement(891..2198)
                     /locus_tag="VFB80_24455"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN37014.1"
                     /translation="MSRKRQDRQDQQAGASDSPRAERLQKVLAAAGVGSRRDCEEMIV
                     QGRITVDRQIVTELGTRVDPLQQEIRVDGEALRRPQRLYYVVNKPVGVVSTSDDPAGR
                     TRVIDLVPTEERVFAVGRLDRSSEGLILVTNDGEFANRITHPRYGVEKTYAVRVAGAA
                     EPKQLARLKKGVHLAEGFAKAQAITVKRKHGQSTDLVIVLNEGRNREVRRILARVGHK
                     VLALKRIAVGPIKLADLPVGAWRRLMPNEVELLLSAAQEKRRAAKRGSGVGGRASEEK
                     SKVQGSKFKVAEEQYVQKAALLAEPLSLDDLLRDDLDEANVGGPLVEDAGTEVTVEEG
                     GELVRADVIDYPDEAPPPPVNAPRQQPERGKFQKQAGGKRDRGKRVGGKRVGDKRAGD
                     KRIGGKRGGGKPGFRAKHARTGKNFQQKQRRGKRDGQRKGRRG"
     gene            2465..>3390
                     /locus_tag="VFB80_24460"
     CDS             2465..>3390
                     /locus_tag="VFB80_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37015.1"
                     /translation="MALLLSRRQFALGSLAAGASLALRSAQAAPYGSYAAIVPGTGVR
                     IARTGDDFEAEDWAYYPQHPKSSFNIDDRVREPGAYSKNNLWVEGAKRGTPDYVRRVQ
                     TPPGGPVGSHGAMLVQSLNSGVPGRLSHEQQQDDLLHNVAGALGSTIPVSWSPNCVCR
                     VFIAPASRWEYRDGATFGYRIGCLGFGKKSKEEEYWPGFFLHQERGIKDGERTFQVRT
                     WVRADGGGRDLPSLVFKPETWITLGMSVTPDGACHFFAREGIDDLTKDDCVGSHWCYG
                     YRAHSFQTFFYNVINQDDGRNVGTPWIIDDAFL"
BASE COUNT          511 a         1187 c         1100 g          592 t
ORIGIN      
        1 ttccgcgtcg aacaccgggc cttccacgca ggtccgcttg tagtcccagc cgccggcggg
       61 ttgcttcacc ttggcgacgc acgtgaagca aatgccgatg ccgcaggcca tcggcgtttc
      121 caatgagacc tggcagggga cctgcgccgc gtgtgcgatc ttggcgacgg cctccatcat
      181 cggctccggg ccgcaacaga cgatccgcgg ccggccgtga tgctccgcca gaacttgctt
      241 aagcaggtcc gagacgaagc cgcgatgccc gcggctgccg tcgtcggtcg ccagccgcac
      301 gtcgataccc agccgctgga aatcctcaac gccggcgagg taatcggccg tgcgagcgcc
      361 ataacaaaac gtgacgcgaa gcgcccgcgg cgcgtcgcgc ggcggatcgc catagcgctt
      421 cttgtgcagg gcctcctggg ccagcgccag aaacggcgtc tgaccgatcc cgcccgcgac
      481 catgatgagg tgctcagtgg gaatgggccc gggtgagtgg tgagtggtga gtggtgagtg
      541 gtggcagaag ccattgccca gcgggcccca gacgtcgagg cgctggccgg gctgcagctg
      601 ccacagccgg ctggtgagct tgcccttcac caggtagacg acatcgacgg cttcgagctc
      661 gccggcggcg gaaagcgccg tgtcatagag cgccagcggc cggccgatca gcggatcgtt
      721 cagatccgcg atccgcagca tgacgaactg ccccggcgtg atgcggcggg cgatctgggg
      781 cgcgtggaag cggacgcgat aggtgtccct ggccaggcgg acgttttcta cgatgggggc
      841 cgcgacttgc accgcctggt cggcgtagaa ctcggcgtgc agggggctcg tcatccgcgg
      901 cgtccctttc gctggccgtc acgcttgccg cggcgctgct tttgctgaaa gtttttcccc
      961 gtccgcgcgt gcttggcgcg aaaccccggc ttgccgccgc cgcgtttgcc gccaatacgc
     1021 ttgtcgccgg cacgcttgtc gccgacacgc ttgccgccga cacgcttgcc gcgatcgcgc
     1081 ttgccgccgg cctgcttctg gaacttgcca cgctcgggct gctgccgcgg cgcgttgacc
     1141 gggggcggcg gggcctcatc cgggtaatcg atcacgtccg cccgcacgag ctcaccgccc
     1201 tcctctacgg tgacctccgt gcccgcgtct tcaaccagcg gtccaccgac gttcgcctca
     1261 tccaaatcgt cccgcaacaa gtcatccagc gacagcggct ccgccaacag cgccgccttc
     1321 tgcacatact gctcttccgc aactttgaac tttgaacctt gaaccttgga cttctcttcc
     1381 gacgcccgac ccccgactcc cgacccccgc ttcgccgccc gccgcttctc ctgggccgca
     1441 ctgagcagca actcgacctc gttgggcatc agccgccgcc acgccccgac gggcagatcg
     1501 gccagcttga ttgggccgac ggcgatccgc ttgagggcca gcaccttgtg cccgacgcgg
     1561 gccaggatgc ggcggacttc gcggttgcgg ccttcgttga gcacgatgac caggtcggtg
     1621 ctctggccgt gcttcctctt gacggtgatc gcctgggcct tggcgaaacc ttcggccagg
     1681 tgcacgccct tcttcagccg ggccagttgc ttgggctcgg ccgcgccggc gacgcgcacc
     1741 gcgtaggtct tttcgacgcc gtagcgcgga tgcgtgatgc ggttggcgaa ttcgccgtcg
     1801 ttggtaacca ggatcaggcc ctcgctgctg cggtcgagcc ggccgacggc gaacacccgc
     1861 tcctcggtcg gcacgagatc gatgacccgc gtccgccccg ccggatcgtc gctggtcgag
     1921 accaccccca cgggcttgtt gaccacgtag tacagccgct gcggccggcg gagcgcctcg
     1981 ccatcgacgc ggatctcctg ctggagcgga tcgacgcgcg tccccagttc ggtcacgatc
     2041 tgccgatcga ccgtgatgcg gccttggacg atcatctctt cgcagtcccg ccggctgccg
     2101 acgcccgccg ctgccagcac tttttgcagg cgctcagcgc gcggcgaatc gctggcgcct
     2161 gcttgctgat cctggcggtc ttggcgtttg cggctcatga gtcgtcagcg atagtcgcca
     2221 tcgcctccgg ctgggggtta aacgacgggt taattctttg cttcccgcat aatttaccgc
     2281 cgcggccgcg ccacgcgaac cgggcataac gtcagggcgc aacgtgccgg cgcgaattgc
     2341 tggacggctt ggcgcgtccg atccgatatt tccgcagtcg atctacccgc cttgggccaa
     2401 ccgtcgcgga cgctgccggt ccggcaaagt ctgcggccag gccacacgtt cagggagcca
     2461 ttctatggcg ctgctcctgt cccgccgtca gtttgcgctg ggttcgctag ccgcgggcgc
     2521 ctcgctcgcc ctgaggtctg cccaggcggc gccgtacggc agctatgcgg ccatcgtgcc
     2581 cggcacgggt gtccggatcg cgcggacggg tgacgacttc gaggccgagg actgggccta
     2641 ttacccgcaa catcccaaga gcagcttcaa catcgacgac cgcgtccgcg agccgggggc
     2701 gtattcgaaa aacaacctgt gggtggaagg ggccaagcgg ggcacccccg attatgtccg
     2761 ccgcgtgcag acgccccccg gcggcccggt cggcagccac ggcgcgatgc tcgtccagtc
     2821 gctgaattcc ggcgtccccg gcaggctatc ccacgaacag cagcaggacg acctgctcca
     2881 caacgtggcc ggcgccctcg gcagcacgat cccggtgagt tggagcccca actgcgtctg
     2941 ccgcgtgttc atcgccccgg ccagccgctg ggaataccgc gacggggcca ccttcggcta
     3001 ccgcatcggc tgcctgggct tcgggaagaa atcgaaagag gaggaatact ggccggggtt
     3061 cttcctgcat caggaacgcg ggatcaagga tggcgagcgg acttttcaag tgcggacgtg
     3121 ggtccgggcc gacggcggcg gccgcgacct gccctcgctc gtcttcaagc cggaaacgtg
     3181 gatcacgctg ggcatgtcgg tgactcccga cggggcctgc cacttcttcg cccgcgaggg
     3241 gatcgacgac ttgaccaagg acgattgcgt cggcagccat tggtgctatg gctaccgggc
     3301 ccacagcttc cagacgttct tctacaacgt catcaaccag gacgacggcc gcaacgtcgg
     3361 cacgccctgg atcatcgacg atgcgttcct
//
LOCUS       DASRJB010000927        12143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3094543, whole genome shotgun sequence.
ACCESSION   DASRJB010000927 DASRJB010000000
VERSION     DASRJB010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094543"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFB80_24465"
     CDS             <1..1462
                     /locus_tag="VFB80_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37016.1"
                     /translation="ENAKRYQLQALFIVNGNRSAANYDNPYPELADLFAAIGPLLESD
                     SADLVGRRLTGAAATYYNASYGSNGTGQLGYATPGASTAVGRSIARFWEAAEKAGDER
                     AVRLSVEAAANVIHEGVQNKLLEYSIKGPEALRPIAASSLSDPRAVILPTSPEFVGPL
                     IERIHANARTDDGRRQVTRSTVRQLSQARWDMPTSEGRQREFFNLLIPKLDDPASDTQ
                     WFLAEQLGRILAANPDFHTPTLLAMAPKEFSSPLEESFWLPSAGWMLTYNARLPEVGQ
                     SAAADGSSELHRYALGLVTRNLDPSADRRARAVAVSMLYQPALYSTPEVIAAAGRIDA
                     GSLRRLLPEAFERDLMRAVSEDAAEPKLELNDRRRRNFTYFRDFVVPELARENRGDGN
                     SCFSCHGGGKIPSMSLEAPDRRTRYLSPAGIWSNYRTLLERIDKSDPAGSKVLRKPLN
                     IQTGKEDGHQGGLRYKPGDRGYEILKRWATDAAELK"
     gene            1566..3065
                     /locus_tag="VFB80_24470"
     CDS             1566..3065
                     /locus_tag="VFB80_24470"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZN37017.1"
                     /translation="MLGHWSLAAAEPLTLHLRSRQETAAGSGRFHAITKDAEWNPSET
                     AIVVCDMWDDHTCPNAAQRVAQMAPRMNEVLKAARKRGVLIIHCPSDTMAFYKDHPGR
                     KLALAAPPVATERPLIRWCYLDKEREGQLPIDDSDPCDCARTWKKGDPYPWTRQIATL
                     EIMDGDAITDSVEAYYLMRQRGIKNLIVMGVHTNMCVLGRPFSIRQMVYQGMNVALMR
                     DLTDTMYNPAQAPFVSHFTGTDLVVQHIERFWCPSLTSGDFLDGKEYRFPADTRPTVA
                     ILLGEEEYQTATTLPKFAAEELGKDYRVPLIFLDETSKASFPGLDAVASADLLLISAR
                     RRPLPPAELAVIKKHVSAGKPVIGIRTASHAFHLRNQPPPAGLADWPTLDADVFGGSY
                     TNHHAPRLKATIWPLAAAREHAILKGIDATQFPGGGGLYQTAPLAAGATELLRGKVEG
                     VAEEQPVAWTFRRADGGQSFYTSLGHPADFERTEFRTLLKNALAQLTRP"
     gene            3086..5566
                     /locus_tag="VFB80_24475"
     CDS             3086..5566
                     /locus_tag="VFB80_24475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37018.1"
                     /translation="MRFTLCLLFLPMALCGQELAPWNSNRHPSDAPKRHVQEIAAGKQ
                     EYRVAQGGTMDGRNCRSPQGVWTPFEQTWESNRSVRMENVGQSDVVNPWLSNGSNDFR
                     TIQAIVARAAQPDMSDREKAMALWWQQVQLRFHYEGDNRELASPVKVLNVYGHNTCGN
                     DSICLAGLWKAAGLKVAPARLVGHCVTQVFYDGGWHLFDGDMHSLYLLRDNETIAGEQ
                     DLVRDHDLIKRTHTQGILRPAARAGDEWEASIYVFEGEVAGDRNADKTPAMNMTLRPG
                     EALVWRWGHTEPAKCFGSARHKFAERVCNGLWEYRPNFSKAAWRKGADSAEGFVSVAD
                     GLAAEEGKTGEIIWTIRSPYVLVGGKLEVQGIGALLDLSWDGKTWEQAGPDLDPFFPP
                     VGTGRYTYQLRCRLSGAARLRSLAIVNDLQMAPLSLPEMAVGENQFTYTDESQGERRV
                     RITHEWVERSASAPPAAPAVPVRPTDGGEAAGTDVVFEWQPAADPDGEPIADYHFELS
                     ARADMKWPLSMSFARLISRTSDSGAPRFTVQAPGELNPDRQYYWHVRAKDARGVWGDW
                     SKTWSFTPRGPAPPQNLALDYDAEKSVGILRWKPNSLGRRPAAYRVYASDEKGFSVSD
                     ETFRVAAGLYDFRAKTATESPTQFPGNFLAETKATQLTVIGPGLALAANKAFYRVVAV
                     DDDGKRSGPSDYVAAPRPVIHSKPPAQTRTGEEFRYDVSAIRSLGDMRTRVVDGREKM
                     NYWDVEQPRFEIVRGPAWLSIDPATGRLSGKPDRAGRAEVVVATKLEREQRSLDPAHL
                     QWGIEKATQSRVETVGTARQSFMIEIAP"
     gene            complement(5581..7176)
                     /locus_tag="VFB80_24480"
     CDS             complement(5581..7176)
                     /locus_tag="VFB80_24480"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37019.1"
                     /translation="MTVLLDRGRVEIKLADYGTAPATALADPLLLGRVQDYRRGAAAR
                     LYPLDGPAILEKVPLGEFHVSRKIDGEFTVLVFRQGEAFTLNPGGTVRVGLPALEEAA
                     RLLSKAGVKEAMLACELYAAREDRRSRVHDIVSLAGKPSKTAELEQMHLAAFDIISLN
                     GSPPPVAFAQTWQQISKLLDKGQRIRPVEARWLEKPDAIRELYDEWVEKEGAEGIVCR
                     SDAAGSFKIKPRHNLDVAVIGFAESSGDRTGLLHDMLVALLRKEGTFHVLGRVGGGFT
                     DEQRKELLSDLKDMVVESEYSEVSSDHVAYQMVRPEWVVEISCLDLVSQNTRGGTVGR
                     MVLDWHANNGHSIYRALRRLPLASVISPQFVRRRDDKQVQPTDVRISQVADLVELPQA
                     EKDARQLIYPQSELLRREVFVKELKGEQMVRKLVLWKTNKEQVAEDFPAYVVHLTDFS
                     PNRKDPLAREIRVSSSLEQIQQLCEQLKAENITKGWNSYTSAIPATPAPPAKEPESPS
                     RKFAASETEPPPKPKPKGRKKSG"
     gene            complement(7173..7916)
                     /locus_tag="VFB80_24485"
     CDS             complement(7173..7916)
                     /locus_tag="VFB80_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37020.1"
                     /translation="MPEISLFNSAGRDAVVNLQSVTTPLRVRWLDPQGRQVGTARVVK
                     STLDRDLEALIEKHGDLRAIGAAIRDQDPELDIETAGRLLSETSRVYVDRGRKVVHRA
                     QEWEVIRNPDGTERTRRRKAIPDPNVGGETPPLKWSGVLIPKREAVRKFVFVQKMQLA
                     HINGLTYDFLFAIAKELESKESLLLLGGGTKSNQPLILRRGGSQFRGFLEGRTKGETY
                     RLVLHLSNLELKAPEEKDSPEQPAEEKAT"
     gene            complement(7965..8582)
                     /locus_tag="VFB80_24490"
     CDS             complement(7965..8582)
                     /locus_tag="VFB80_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37021.1"
                     /translation="MAKPLVFQWGDRQLSFQMNKVDRAKLYGFKESEVLDDKGQKCEL
                     ATLASDGQTVVGRGGSGFGNLTVDGAWIEKAQLTPVDPAGQKVQPVPSSFAAPVPLNE
                     KVPPEDYLDCAVRSIYQLQSPDDVGPLAAELRQGVIYRFPYSFRGGLEPDQGFLLANA
                     EGELFCAIGKPTKVEFLSLAQAAAAYEEEEAGEEDEADAMDFSLM"
     gene            8818..9942
                     /locus_tag="VFB80_24495"
     CDS             8818..9942
                     /locus_tag="VFB80_24495"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN37022.1"
                     /translation="MPRQIVICGKLVSMTRADAARIAAAHGDELTEHLSRTTDLVVLG
                     GGSPPISRGGRVTPQFARVRRLLRQGVLLKVVPEEQWLDELGLTRQAEGVRGSFTANQ
                     LAEMLKLPRPQLDRWIDAGLIRATGDNAGLPQFDFRQVAAARLLVDLAAAGIRLNKVR
                     RAVAQLSRWLPGASDPLAELCLEDDAGRLIVRTKDGRAAEPSGQLLMDFEPPADAATL
                     AFCQSESDADAFRRAVDCEELDPLAAAALYRQLIAAHGPHATLAFNLGNALYAADDLP
                     AAVEQLRHATALDPGHAGAWNNLANLLAESDQLDEASSAYRRALALDPDCSEARFNLA
                     QTLVELGRADEAVLHWRAYLAADADSTWADYARERLEERG"
     gene            9950..11503
                     /locus_tag="VFB80_24500"
     CDS             9950..11503
                     /locus_tag="VFB80_24500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN37023.1"
                     /translation="MLRYHGRMMHPKAVGLLAVLVIAATAWRIQQARESLWLDELHSA
                     WCAGGDLREVAPRAAIGNQSPLYFWLLWGQTQLYSQSELVLRGPSLTAGVLTLVAVYW
                     LVARTLRSPWLGLLAAWLYALIPLADFYATEARPYALVTLIAVLHVAIFAALCRQPTW
                     QKRALFVLGAALLFHLHYTAALLLPAELAFWLVAAGCRLMPIRYSSRQMTWDLVFIGL
                     AWMPAAGLIGVILGRRQNWEAFVPQRPLWEIFELLPWSAAALVVLGLLPFDRYLAGQR
                     RQPGVVTRNQLRLRRAAALGLCWLLVPLLAAWAATWTDTARLLLPRYLVASMPAAVLL
                     AVTCVRFAPWQWSQVAAGSLLAGAALWMSLVSQPARRGEDWRGAIEWLNEQLAEREFP
                     VLVMSDLIEDQGLRASPDERLKHYCLFPVTSLYRVNLPQSELVPLPSRGHGILTSEQC
                     EMVRSRGGAWLIVRTKELETANTIGREVGKELSSDAPDDRVWRLAANHRGEGVHSVLL
                     LPRLPTPDP"
     gene            11585..>12143
                     /locus_tag="VFB80_24505"
     CDS             11585..>12143
                     /locus_tag="VFB80_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37024.1"
                     /translation="MTATAAKESKPFEAPHPGTKAMPQWDTGDLIDAPIFTTRNLLAM
                     IGPGLVMGASAIGGGEWLAGPAVTAKYGGALLWVATVSIFFQVIYNIEISRYTLYTGE
                     PIFTGKFRLPPHPMFWLVVYLMLDWGSVAPYLAANAAIPVDTMILGRLPNVANLSGDW
                     WLHKCICTVLYLLVTVPLIFGGKIYN"
BASE COUNT         1991 a         4104 c         4017 g         2031 t
ORIGIN      
        1 cgaaaacgcc aaacgctacc agcttcaggc cctcttcatc gtcaacggca accgctcggc
       61 ggcgaactac gacaatccgt acccggagct ggccgacttg ttcgccgcga tcgggccgct
      121 gctcgaatcg gactcggccg acctggtcgg ccggcggctg accggtgcag ccgcgaccta
      181 ctacaacgca tcctacggct ccaacggcac cggccaactg ggctacgcca cgcccggcgc
      241 gtccacggcg gtcggccgct cgatcgcccg tttttgggaa gcggccgaga aggcgggaga
      301 cgagcgggcg gttcggctgt cggtcgaagc ggccgccaac gtgatccacg aaggggtgca
      361 gaacaagctg ctggagtatt cgatcaaggg accggaggct ctgcggccga tcgccgccag
      421 ttcgctctcc gatccgcggg cggtgattct gccgacttcg cccgaattcg tcggcccgct
      481 gatcgagcgg attcacgcca atgcccggac cgacgacggc cgccgacaag tcacgcggtc
      541 caccgtgcgg cagctgtcgc aagcccgctg ggacatgccc acgtcggaag gccgccagcg
      601 cgagttcttc aacctgctca ttcccaagct ggacgacccg gccagcgaca cgcagtggtt
      661 cctggccgag cagctcggcc gcatcctggc tgcgaacccc gacttccaca cgccgacgct
      721 gctggcgatg gcgccgaagg agttcagcag cccgctggaa gaatcgttct ggctgcccag
      781 cgccggctgg atgctcacct acaacgcacg gctgcccgag gtcggccagt cggccgcggc
      841 agacggttcc agcgagctgc accgctatgc gctcggactc gtcacgcgaa acctcgatcc
      901 cagcgccgac cgccgggccc gcgcggtggc ggtttcgatg ctctatcagc cagccctcta
      961 ttcgaccccg gaggtgatcg ccgccgccgg ccgcatcgac gccggcagtt tgcgccggct
     1021 cttgcccgag gcgttcgagc gcgacctgat gcgggcggtg agcgaggatg ctgctgagcc
     1081 gaagctggag ttgaatgacc ggcggcggcg gaatttcacc tatttccgcg acttcgtcgt
     1141 gcccgaattg gcccgcgaga accgcggcga cggcaactcc tgcttctcgt gccacggcgg
     1201 cggcaagatt ccgtcgatgt cgctggaggc tcccgaccgc cgcacgaggt acctgtcgcc
     1261 ggcgggcatc tggagcaact accgcacgct gctggagcgg atcgacaaga gcgacccggc
     1321 ggggagcaag gtcctccgca agccactgaa tattcagacc ggcaaggaag acggccacca
     1381 gggcggcctg cgctacaaac ccggcgaccg cggctacgag attctcaagc ggtgggcaac
     1441 ggatgcagcg gagctgaagt gaagcatcaa attacaaacc agcaccaaac ttcaaaatga
     1501 gaatgttcca aaccatgccg tttcgatcat taagccttcg aacattggga ttggtttgtt
     1561 atttggtgct tggtcattgg tcattggcag ccgcggagcc gctgacactg cacctgcggt
     1621 cccggcagga gacggccgca ggatcgggcc ggttccacgc catcacgaaa gacgctgagt
     1681 ggaatccgag cgagacggcg atcgtcgtct gcgacatgtg ggacgaccac acctgcccca
     1741 acgcggcaca gcgcgtcgcg cagatggccc ctcgcatgaa cgaagtcctg aaagcggccc
     1801 ggaagcgcgg cgtgctcatc atccattgtc ccagcgacac gatggccttc tacaaggatc
     1861 atcccggccg caagctggcc ctggcggccc cgcccgtcgc cacggagcgg ccgctgattc
     1921 gctggtgcta cctcgacaag gagcgcgaag ggcagctgcc gatcgacgac agcgacccct
     1981 gcgattgcgc gcggacctgg aagaagggcg atccgtatcc ctggacgcgg cagattgcca
     2041 cgctcgagat tatggacggc gacgccatta ccgattcggt cgaagcgtac tacttgatgc
     2101 ggcagcgggg catcaagaac ctgatcgtga tgggcgtgca cacgaacatg tgcgtcctcg
     2161 gccggccgtt ctcgatccgg cagatggtct atcaggggat gaatgtcgcc ctgatgcgcg
     2221 acctgaccga cacgatgtac aacccggccc aggcgccgtt cgtcagccac ttcaccggca
     2281 ccgacctggt cgtacagcac atcgagcggt tctggtgccc gtccttgacc agcggcgatt
     2341 tcctggacgg caaggaatat cgtttcccgg ccgacacgcg gccgacggtg gcgatcctgc
     2401 tgggcgagga ggaatatcaa acggcgacca cgctgcccaa gttcgccgcg gaagagctcg
     2461 gcaaggacta tcgggtgccc ttgatctttt tggacgagac gagcaaggcc agcttcccag
     2521 gcttggacgc cgtggccagc gccgatctat tgctgatcag cgctcgccga cgcccgctgc
     2581 cgccggctga gctggcagtt atcaagaaac acgtctcggc cggcaagccg gtgatcggca
     2641 tccgcaccgc cagccacgcg tttcaccttc gcaatcagcc gccgccggcc gggctggcgg
     2701 attggccgac actcgacgcg gacgtctttg gcggcagcta cacgaaccat cacgccccgc
     2761 ggctgaaggc gacgatctgg ccgctggcag cggcgcggga gcacgcgatc ctgaagggca
     2821 tcgacgcaac gcagtttccg ggcggcggcg gactctatca gaccgcgccg ctggcggccg
     2881 gcgcgacgga attgctccgc ggcaaggttg agggcgtggc cgaggagcag ccggtggcct
     2941 ggactttccg ccgcgccgac ggcggccagt cgttctacac gtcgctgggc caccccgccg
     3001 actttgagcg caccgaattt cgcacgctgc tgaagaacgc gctggctcag ctgacccgcc
     3061 cctaaacccg accgcggaag atgccatgcg attcacgctc tgcctgctgt tcttgccgat
     3121 ggcgctctgc ggccaggagc tcgcgccgtg gaattccaat cgccacccca gcgacgcacc
     3181 gaagcggcac gtgcaggaga tcgctgccgg caagcaggaa taccgcgtcg cgcagggcgg
     3241 cacgatggac gggcggaatt gccgctcgcc gcaaggcgtc tggacgccgt ttgagcaaac
     3301 ctgggaatcg aaccgcagcg tgcggatgga aaacgtcggc cagtcggacg tcgtcaaccc
     3361 ctggctgtca aacgggagca acgacttccg cacgattcag gccatcgtcg cccgcgcggc
     3421 ccagcccgat atgagcgacc gcgaaaaagc gatggccctc tggtggcagc aagtccagct
     3481 gcggtttcac tacgaagggg acaaccgcga attggccagc cctgtgaagg tgctgaacgt
     3541 ctacggtcac aacacgtgcg gcaacgattc gatctgcctg gccgggctgt ggaaggcggc
     3601 gggcctgaag gtggccccgg cccgcctcgt ggggcattgc gtcacgcagg tcttctacga
     3661 cgggggctgg cacctgttcg acggcgacat gcactcgctg tatctgctgc gcgacaacga
     3721 aacgatcgcc ggcgagcagg acctcgtccg cgaccacgac ctgatcaagc ggacgcacac
     3781 gcagggcatc ctccgcccag cggcgcgggc cggcgacgag tgggaagcgt cgatctacgt
     3841 gttcgagggg gaagtcgccg gcgaccgcaa cgccgacaaa accccggcga tgaacatgac
     3901 gctgcggccc ggcgaggccc tcgtctggcg gtggggccac accgaacccg cgaaatgctt
     3961 tggctccgcg cgccacaagt tcgccgagcg ggtctgcaac gggctctggg aataccggcc
     4021 gaatttctcc aaggcggcgt ggcgaaaggg cgcggattcc gccgagggct tcgtctcagt
     4081 cgcggatgga ctggcggccg aggaaggaaa gaccggtgag atcatctgga ccatccgctc
     4141 gccgtacgtg ctcgtgggag ggaagcttga agttcaggga atcggcgctc tgctggatct
     4201 ctcgtgggac ggcaaaacat gggaacaggc cggccccgat ctcgatcctt tcttcccgcc
     4261 ggtcgggaca gggcgctaca cctaccagtt gcgctgccgc ctctccggcg cagcgcggct
     4321 ccgcagcctg gcgatcgtga acgacctgca gatggctccg ctgtcgctgc cggaaatggc
     4381 cgtcggcgaa aaccagttca cctacaccga cgaatcacag ggcgagcgcc gcgtgcggat
     4441 cacgcacgag tgggtcgagc gctcggccag cgcgccgccg gctgccccgg ctgttcccgt
     4501 ccgtcccacg gacggcggcg aggccgcggg gacggacgtg gtcttcgagt ggcagccggc
     4561 ggccgatccg gacggcgagc cgatcgccga ctatcacttt gagctctccg cgcgggccga
     4621 catgaaatgg ccgctctcga tgagttttgc gaggctgatc tcgcgcacca gcgactccgg
     4681 cgcgcctcgc ttcacagtcc aggcccccgg cgagttgaat cccgaccgcc agtactactg
     4741 gcacgtgcgg gccaaggatg cccgcggcgt gtggggcgat tggagcaaaa cctggagttt
     4801 cacgccgcgc gggcccgcac cgcctcagaa cctcgccctg gactacgacg cggagaagag
     4861 cgttggcatc ctgaggtgga agcccaactc gctggggcgc cggccggcgg cctatcgagt
     4921 ctatgccagc gacgaaaagg gattctcggt cagcgacgag actttccgcg tcgcggctgg
     4981 cctgtacgac ttccgagcca agactgcgac cgagtcgccc acgcagtttc ccgggaattt
     5041 cctggcggaa acgaaggcta cccagctgac tgtgattggg ccgggcctgg cgctggcggc
     5101 gaacaaggcc ttttaccgcg tcgtcgccgt cgatgacgac ggcaagcgga gcgggccttc
     5161 ggactatgtt gccgcgccgc ggcccgtcat tcacagcaag ccgccggccc agacgcgaac
     5221 cggcgaagag tttcgctacg acgtgagcgc cattcgctcg ctgggcgaca tgcggacccg
     5281 cgtcgtcgat ggccgggaga aaatgaatta ctgggacgtc gagcagccgc gattcgaaat
     5341 cgtgcgcgga ccggcgtggc tcagcatcga cccggccacc ggcaggctgt cgggcaaacc
     5401 agaccgcgcc gggcgggccg aggttgtcgt cgccaccaag cttgagcgcg agcagcggag
     5461 cctcgacccg gcgcatctgc agtggggcat cgaaaaggcc acgcagtcgc gcgtggaaac
     5521 tgttggcacg gcccggcaga gttttatgat cgagatcgcg ccgtaattgc ggccgtccga
     5581 tcacccggac ttcttccggc ccttcggctt gggcttgggc ggcggctcgg tctcgctcgc
     5641 ggcgaacttg cggctggggg attccggctc tttcgccggc ggggcaggag ttgcgggaat
     5701 cgccgacgtg tacgaattcc agcccttggt gatgttttca gccttgagct gctcgcagag
     5761 ctgctggatc tgctccaggc tgctggagac gcgaatctcg cgggccagcg ggtccttgcg
     5821 gttggggctg aagtcggtca ggtgcaccac gtacgccgga aagtcctcgg ccacctgctc
     5881 cttgttggtc ttccagagca cgagcttgcg gaccatctgc tcgcccttga gctccttgac
     5941 gaacacttcg cgccgcaaga gctcgctctg gggatagatg agctgccggg cgtccttctc
     6001 ggcctgcggc agctcgacca ggtcggccac ctggctgatc cgcacgtccg tgggctgcac
     6061 ctgcttgtcg tcgcggcggc ggacgaattg cggcgagatc acgctggcca gcggcaggcg
     6121 gcggagggcc cggtagatcg aatgcccgtt gttggcgtgc cagtcgagga ccatccggcc
     6181 gacggtgccg ccgcgagtgt tctgcgagac caggtcgaga cagctgatct cgacgaccca
     6241 ctcgggccgc accatctggt aagcgacgtg gtcggagctg acctcggaat actcgctctc
     6301 gacgaccatg tctttcagat cgctgagcag ctcctttcgc tgctcgtcgg taaagccgcc
     6361 gccgacgcgg cccagcacgt ggaacgtgcc ttccttgcga agcagagcaa ccagcatgtc
     6421 gtgcaagagg cccgtacggt cgcccgacga ctcggcgaag ccgatcaccg ccacgtcgag
     6481 gttgtgccgc ggcttgatct tgaagctgcc ggcggcgtcg ctgcggcaga cgatcccctc
     6541 ggccccttcc ttctcgaccc actcgtcgta cagttcgcgg atggcgtcgg gcttctccag
     6601 ccagcgagcc tcgaccgggc ggattcgctg acccttgtcg agcagcttcg agatttgttg
     6661 ccaggtttga gcaaacgcca ccggcggcgg cgagccattc agcgagataa tgtcgaacgc
     6721 cgccaggtgc atctgctcca gctcggcagt cttcgacggc ttgccggcca ggctgacaat
     6781 gtcgtgcacg cggctgcggc ggtcctcgcg ggcggcgtac agctcgcagg ccagcatggc
     6841 ctccttgaca cccgccttcg agagcaggcg ggccgcttcc tccagcgccg gcagcccgac
     6901 gcggacggtg ccaccgggat tgagcgtgaa ggcctcgccc tggcgaaaga cgagcacggt
     6961 gaattcgccg tcgatcttgc ggctgacgtg gaattcgccc agggggactt tttcgaggat
     7021 cgccgggccg tcgagcggat agagccgcgc ggccgccccg cggcggtagt cctgcacgcg
     7081 gcccagcagc agcggatcgg ccagcgcggt ggccggcgcg gtgccgtagt cggcgagctt
     7141 gatttcgacg cggccgcgat cgagcagcac tgtcatgtgg ccttctcctc cgcgggctgc
     7201 tcgggagaat ccttttcctc gggcgccttc agctccaggt tcgacaggtg tagcaccagg
     7261 cggtacgtct cacctttggt ccggccttcc aggaagccgc ggaactggct gccgccgcgg
     7321 cggaggatca gcggctggtt cgacttggtc ccaccgccca agagcagcag gctctccttc
     7381 gattccagct ccttggcgat cgcaaacagg aagtcgtagg tcaggccgtt gatgtgggcc
     7441 agctgcattt tctggacgaa gacgaatttg cggacggcct cgcgcttggg aatcaggacg
     7501 ccgctccatt tcagcggcgg ggtctcgccg ccgacgttgg gatcgggaat cgccttgcgg
     7561 cggcgggtcc gctcggtgcc atcgggattg cggatgactt cccactcctg cgcgcggtga
     7621 acgaccttcc gcccgcgatc gacatagacc cgcgacgttt cgctcaggag acgtccggcc
     7681 gtctcgatgt ccagctccgg gtcctggtcg cggatcgccg cgccgatcgc ccgcaggtcg
     7741 ccgtgctttt cgatcaaagc ctccaggtcg cggtcgagcg tcgatttgac gacccgcgct
     7801 gtgccgacct gccgcccctg cggatcgagc caccgcaccc gcaggggcgt cgtgaccgac
     7861 tgcaggttta cgaccgcatc ccgcccggct gaattgaaca aactgatttc tggcatagcc
     7921 gactcccgcc aactgactac tgaccactga caactgacac ccgcctacat caaactgaaa
     7981 tccatcgcgt cggcctcgtc ctcttcgccg gcctcctctt cctcgtaggc cgcggccgcc
     8041 tgggcgaggc tgaggaactc gaccttcgtc ggcttgccga tggcgcagaa cagctcgccc
     8101 tcggcattcg ccagcaggaa cccctggtcc ggctccaacc cgccgcgaaa gctgtagggg
     8161 aagcgataga tcacaccctg ccgcagttcg gcggccagcg gcccgacgtc gtccggcgac
     8221 tggagctgat agatcgaccg cacggcgcag tcgagatagt cctcgggcgg gaccttttcg
     8281 tttagcggca ccggcgcggc gaacgacgag gggaccggct gcaccttctg ccccgccgga
     8341 tcgaccggag tgagctgggc cttctcgatc caggcgccgt ctacggtcag attgccaaag
     8401 cccgagccgc cgcggccgac gacggtctgg ccgtcagagg ccagcgtcgc cagctcgcac
     8461 ttctggccct tgtcgtcgag cacctcactc tctttgaagc cgtagagctt cgcccgatcg
     8521 accttattca tctggaagct gagctggcga tcaccccact ggaagacgag cggcttggcc
     8581 ataaaatcac ctcacgcccc ggcgcgaatg gaccccgagg gaccgctagc ttaacacgct
     8641 ggcagcgcgg tgctagcaaa cttgacgcct gtacccgttc ggcttggggg gctggaaacg
     8701 ccgagccggc cgaatctgcc gtttgggtaa gctgttcggt ctcacgctta acccgggagc
     8761 ccgaggcggg gatccggaga ccaacgaagg caaagcgcat ccgaaggacg ccggcaaatg
     8821 ccgcggcaaa tcgtcatttg cggaaagctc gtgtcgatga cgcgggccga tgccgcgcgc
     8881 atcgcggcgg cgcacggcga cgagctgacc gagcatcttt cgcgcacgac agaccttgtc
     8941 gtgctggggg gcggctcgcc gccgatttcg cgcggcggcc gcgtgacgcc gcaattcgcc
     9001 cgcgtccgcc ggctgctgcg gcagggcgtg ctgctcaaag tggttcccga ggagcagtgg
     9061 cttgacgaac tgggcctgac gcgccaagcc gaaggcgtgc gaggcagttt caccgcgaat
     9121 caactggccg agatgctcaa gctcccgcga ccgcagctcg accgctggat cgacgccggg
     9181 ctgatccgcg cgacgggcga caacgccggc ctgccgcagt tcgacttccg gcaagtcgcc
     9241 gccgcccgcc ttttggtcga tctggccgcc gcgggcatcc gcctgaacaa ggtgcggcgg
     9301 gccgttgcac agctttcgcg gtggctgccc ggggccagcg atcccctggc ggagctctgc
     9361 ctggaagacg acgccggccg gctgatcgtg cggacgaaag acggccgtgc ggccgagccg
     9421 agcgggcaat tgctgatgga tttcgagccg ccggccgatg cggcgacact cgcgttctgc
     9481 caatcggagt ccgacgccga tgccttccgc cgcgcggtgg actgcgagga gctcgatccg
     9541 ctggcggccg cggccctcta tcggcagctc attgcggcgc acgggccgca cgccacgctg
     9601 gccttcaacc tgggcaacgc gctctatgcg gccgacgacc tgcccgccgc cgtggagcag
     9661 ctgcggcacg cgacggcgct cgatccgggc cacgccggag cctggaacaa cctggcgaac
     9721 ctcctggccg agtcggacca gctcgacgaa gcttcttccg cctatcgccg ggccctggcg
     9781 ctcgatccgg attgcagcga agcccgcttc aacctggccc agacgctcgt cgagttgggc
     9841 cgcgcggacg aggcagtgct gcactggcgg gcctacctcg cggccgacgc cgacagcacc
     9901 tgggccgact acgcccgcga acggctggaa gagcgcgggt aatggcctgt tgctacgcta
     9961 tcatggtcgg atgatgcatc ccaaggccgt cggcctgctc gcggtcctcg tcatcgccgc
    10021 caccgcctgg cggattcagc aggcccgcga aagcctctgg ctcgacgagc tgcattcggc
    10081 ctggtgcgcc ggcggcgacc tgcgcgaggt cgcaccgcgg gcagccatcg gcaatcagtc
    10141 gccgctgtat ttctggctgt tgtggggcca gacgcagctg tattcgcaga gcgagctcgt
    10201 gctgcgcggg ccgagtctga ccgccggcgt gctgacgctc gtggccgtgt attggctcgt
    10261 ggctcgcacg cttcgctcgc cgtggctggg gctgctggcg gcgtggctct atgccctgat
    10321 tcccctggcc gatttctatg ccaccgaagc gcggccctat gcgctcgtga cgctgatcgc
    10381 ggtgctgcac gttgcgatct ttgccgcatt gtgccgccag ccgacctggc agaagcgggc
    10441 actgttcgtg ctcggagccg ccctgctgtt tcacttgcac tacaccgcgg cgctgctgct
    10501 gccggcggag ctcgcgttct ggctcgtcgc ggctggttgc cggctgatgc ccattcgata
    10561 ctcttcgcgc caaatgacct gggacctggt attcatcggc ctggcgtgga tgccggcggc
    10621 ggggctgatc ggcgtgattc tgggccggcg gcagaactgg gaggccttcg ttccgcagcg
    10681 gccgctgtgg gagattttcg agctgctccc ctggtcggcg gcagcgctcg tggtcttggg
    10741 gttgctgccc ttcgaccgat acctggccgg gcagcggcgc cagccgggcg tggtgacgcg
    10801 caaccagctc cgcctgcggc gcgcggcggc tttgggtctg tgctggctgc tcgtgccgct
    10861 gctggcggcc tgggccgcga cctggaccga tacggcgcgg cttctccttc cgcgctatct
    10921 cgtggcgagc atgcccgcgg ccgtcctgct cgcagtgacc tgcgtgcgat ttgcaccctg
    10981 gcagtggtcg caagtcgccg ccggatcgct gctcgccggc gcggcgctct ggatgagcct
    11041 tgtctcgcag ccggctcggc gcggcgagga ctggcgcggc gcgatcgaat ggctcaatga
    11101 gcagctcgcc gagcgggagt ttcccgtgct cgtcatgagc gacctcatcg aggatcaggg
    11161 tttgcgggca tcgcccgacg agcggctgaa gcactattgc ctgttcccag tgacctcgct
    11221 ttaccgcgtc aatttgccgc agagcgaact ggttcccttg cccagccgcg gccatggcat
    11281 tttgacgtcc gagcagtgcg aaatggtgcg ctcgcgcggc ggcgcgtggc tgatcgtgcg
    11341 cacgaaggag ctggagacag ccaataccat cggccgcgaa gtcggcaagg agctcagcag
    11401 cgacgcgccc gacgacaggg tctggcggct cgccgccaac caccgcggcg agggagtcca
    11461 ctcggtcctc ctgctccccc gactcccgac tcccgacccc tgactcccga ctcgcgggtt
    11521 gacccacccg ccattgccgc ccatcatacg ctgcgaagct tcgcgcctct cgccccgccc
    11581 cgcgatgacc gctaccgccg ccaaagagtc caagccgttc gaggcgccgc atcccggcac
    11641 caaggcgatg ccgcagtggg acacgggcga cttgatcgac gcgccgatct tcaccactcg
    11701 caacctgctg gcgatgatcg gcccgggcct ggtgatgggc gccagcgcga tcggcggcgg
    11761 cgagtggctc gccggccccg ccgtcaccgc caagtacggc ggggccctgc tctgggtcgc
    11821 cacggtcagc atcttcttcc aggtgatcta caacatcgag atcagccgtt acacgctcta
    11881 caccggcgag ccgatcttca cgggcaagtt ccgtctcccg ccgcacccga tgttctggct
    11941 ggtcgtgtac ctgatgctcg actggggctc ggtcgcgccc tatctcgcgg cgaacgcggc
    12001 aatcccggtc gataccatga tcctggggcg tttgcccaac gtcgccaacc tgagcggcga
    12061 ttggtggttg cacaaatgca tctgcacggt cctgtacctg ctggtcaccg tgccgctgat
    12121 cttcggcgga aagatctaca act
//
LOCUS       DASRJB010000928        17562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4209537, whole genome shotgun sequence.
ACCESSION   DASRJB010000928 DASRJB010000000
VERSION     DASRJB010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4209537"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VFB80_24510"
     CDS             complement(<1..1184)
                     /locus_tag="VFB80_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37025.1"
                     /translation="MRKPCFVGLVLAAAFWTLPAVADEPAKIDERIAFSGTHAQAIEL
                     SAGQTVEISAGIAAPSRLPANGRVAVEWSGPAADSGFRKILHALDPDVYLIYRAPQGG
                     KYTLTLRAVEDEEPPANSPRWRETGVLADVAPFPKQTPWPAGHKVELRLLVQPVDFGT
                     ATRGHIVELEPNNSPAQAQPLPLAAGDGDQILHLTGGADDIEYFDNSRYGESGDDWFR
                     LEYRGDSPRLLSVNLMPTDPIVAARVRVYTPDGADYSEGKHLNETTHEQVEEHRTAVV
                     RTLRPGAVLLLRVEANSPGYDLEIRIRRPAPLADPRAAVQLAMYDHVAQVSAWLMNRP
                     RGNSLDRRLRDTGSMYGSNCMSCHTQSGVWGPAGPLAFGYRIENPLHYRHLMNVMYES
                     LRP"
     gene            1333..2592
                     /locus_tag="VFB80_24515"
     CDS             1333..2592
                     /locus_tag="VFB80_24515"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN37026.1"
                     /translation="MSDSTTRRSFLMQASQAALAPAIATSLAHLASVLAAAPPGGEDY
                     WQVVRQQFDFRDEKAPMNAANLCPSPRAVAQRVTELTRDIDVDCSFQNRAKFAGLLET
                     SRQKVAEQLGVTADEIALVRNTSEANNTINNGLPLKSGDEVVLWDQNHPTNNVAWDVR
                     AARFDLRVRRITTPAAPADAGQLLQAFTDAFGPRTRVLALTHVSNVSGLRLPVAELGE
                     AAQRRGIWVHVDGAQSWGALHVNVRKLNCDSYSASAHKWFVGPKEAGLLFVRQERIAE
                     VWPNVVAPGWGNQVQPEVKGARKFESLGQRDDAALAAIATAAEFHAAIGPAQVESRLI
                     ELATALKTGLKDAGIELVTPLEPSMSGGVCVARVPAAKSNEIYNRLYEEHGIAGATTG
                     GLRLCPHVYNTREHIDRAIRGVKALLS"
     gene            complement(2589..3455)
                     /locus_tag="VFB80_24520"
     CDS             complement(2589..3455)
                     /locus_tag="VFB80_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37027.1"
                     /translation="MAHETFQVGDLTAVIGDNEAYDDRRYGYSGIHRLVHRTRPEPSL
                     LGVAGLNFEHIFDGEKDQLNLSGDRKIFFEPRNHPMEYRRLAADEAELHQTPTPTYHL
                     ESWSRFKLVPPHYVDFTFRCKPHQHAFRYGYIGLFWANYINAPENKSIYFRDSKGWVQ
                     LCTQEHNNQSTVRHVDDKLELRFLETPQQTLYKNLSPLRFAEPFYYGYIGTTHIFILM
                     FDRSEGIRFSHSPSSGGAPSFPNPAWDFQFIVPKYDVLQEYGYRARIAYRERCPREEI
                     EQEFRRWRDSLA"
     gene            complement(3471..5948)
                     /locus_tag="VFB80_24525"
     CDS             complement(3471..5948)
                     /locus_tag="VFB80_24525"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN37028.1"
                     /translation="MTRAIRALSFAVVVLGTAWASAHESEPVDPRAFGAIMPRLAPGG
                     EELAFSYQGAIWRMPRAGGTMQRLTRGGGFDVEPAWAPDGTRLALISGSTFGSGTLTV
                     IDARTGENVKLPREVTAIDRLHFSRDGRRVLGLFQPAGERVRLAWFDLQSGELTAAVA
                     AESWPGQPPGTPGISRQPFALSHDNAALAVVSAADEPGEQSGNRGPQTDLWRVPLGGG
                     KPELIVHWPARIHGLAFRADDQALFIATERGSVHHDLWEVPLAGADAAARKLTFGQAD
                     ESSPSVSDDGRWLLFTDNRLGPTLLALRDLASGQEQIVAPSQRDFGTPKGAVELKVTE
                     GAEHSPTTARIVLRHADGKHHAPSGALYRVLGGDMHFYLQDHVRMELPIGEYTVAAAR
                     GPEHRSVRQTFTVRQGESAKVTLRIERWTNQRSAGWVSGENHIHANYGYGHWYNSPAT
                     MRLQCEGEDLTVANFMVANSDGDGVFDREFFLGRPDPLSTERTILYWNEEFRSTIWGH
                     MTLLNLKRLVVPIFTGFRHTTHPHDVPTNADIADHTHEQGGHVNYTHPANNLQDPYDS
                     AYSAKEMPIDIALGKVDSIDVMGSNHQANLPVWYRLLNCGLRVPASAGTDCFLNRIVS
                     RLPGSDRVYVHCPEKLTYQDWIARLRAGQTFVTNGPMLRFTVNGQEAGNTIKLAGPGK
                     AAVACEASSQFPLEKLEVLVNGQVAATAAAGEKPEQLTLNQEVPLDTSGWIAFRVRGR
                     RPPGMQAAELFAHTSAVYVEVAGRPVRSTEDAEYFIRWIERLRADVRQRNRIPAAQQT
                     QVEQQFSQALDFYRRLAAGSPAGEQSR"
     gene            6035..6226
                     /locus_tag="VFB80_24530"
     CDS             6035..6226
                     /locus_tag="VFB80_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37029.1"
                     /translation="MARVNQQRWQYTLASLFWLMLLVALLAGAWRIGWMKVAATVAIG
                     TGVLFCLVWAAFLFDELRR"
     gene            6223..6363
                     /locus_tag="VFB80_24535"
     CDS             6223..6363
                     /locus_tag="VFB80_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37030.1"
                     /translation="MSDNPYESPQTEPRRRPRPPILAGVVLFAGLAVVALLIAVAVVC
                     VA"
     gene            6586..7329
                     /locus_tag="VFB80_24540"
     CDS             6586..7329
                     /locus_tag="VFB80_24540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN37031.1"
                     /translation="MAISSAYQTLRTSWRGVRRFVKKVPYLGFGRWCSVCGRRSRRFC
                     RHRHFPNPEAKCVHCGAFARHRFFWLYVNAHTDLFDGRPKRMLHVAPEACFIDKMRER
                     LGEGYVTADLDDERAMVKMDITAIQFPNASFDVIYCSHVLEHVDDDRRAMREFYRVLK
                     PGGWAILLVPIDAAKTFEDPSIVDPAERLRLFGSEFHVRRYGPDYVDRLQEAGFEVQE
                     TRIADMFSDDEALRLGIARGAGDIHHCTK"
     gene            complement(7548..8249)
                     /locus_tag="VFB80_24545"
     CDS             complement(7548..8249)
                     /locus_tag="VFB80_24545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN37032.1"
                     /translation="MFPSAAYLNLAGWFHLGFFGVYLPIMAARNRKKVLGSGGALPDR
                     LRHFQRTALEVGLLAICSLLVARVQRIELFPLAFPPLGAVIAGVVAYAAAVAYMRPNW
                     RRAVERRARVVHLFMPSNMRERIWWIIVAVIAGVGEEITWRGTQAALVGALTGSFWAA
                     SLVCSITFGLVHIVQGWKSAALIVVFALGFHLLVWLAGSLYVAMAVHVAYDLTVGISY
                     ERLGKQLGYDPAQGC"
     gene            complement(8301..8786)
                     /locus_tag="VFB80_24550"
     CDS             complement(8301..8786)
                     /locus_tag="VFB80_24550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN37033.1"
                     /translation="MIAVLKTGDRDAQVQAITGLRLLNEPSSLPSLLEALESPYSEVR
                     KAAIGGLWMLRDRKAVGPLIRHLKDDDIEVAAAAAGALGWIEDRQAVAPLLLALENRN
                     WRLRQAAAYALGSIGDDDTLDAVRRHLFDPKPQVRKAVKCGLARFHQRNRNFRSQRRV
                     D"
     gene            9531..10148
                     /locus_tag="VFB80_24555"
     CDS             9531..10148
                     /locus_tag="VFB80_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37034.1"
                     /translation="MRRWLWACSASIGLVLAANSAQADYGTGWGHRFHVDFYRNNTWP
                     EPFRTADRNLAREAFCIQVDNGWKMQNTVGSLLFNPETQQLNQAGDLLVKWIVTQAPV
                     HRRAVFVVKGDNADITRARVDSVNAAVARYAQGPVCPVMLTTVEPEGWPASYIDSITQ
                     QFNSSIPSPRLPGGGGGGGGGGSGGGGGGNGGGGGNGGGSGGSSP"
     gene            10371..11501
                     /locus_tag="VFB80_24560"
     CDS             10371..11501
                     /locus_tag="VFB80_24560"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN37035.1"
                     /translation="MKIQRKLAVTMLLGAGVGLCGCQSPSQGGLAFWKKDSNSSLASA
                     TPDVGAQRYQGLAKEFGSQSTGGATALGAQKPAPDNFLMASWKKTTGAVAGAFAAKPS
                     TTEASDPLRLDQPTKKLGPEVHMGAAQLMENQGKFPEAEEHYQKALKQSPNNLGALVG
                     LARLHDRQGHSRAALETYDRALKAHPQSGLVLNDLGLCLARQKQFDPALGALKRAVQN
                     NPENPKYRNNLATVLVETGRVAEALQTLSAGSSPAVAHYNVGYLLAQRGQTNEAAQYF
                     QQALAIDPALTQARDMLAQLAGAGGTQPMPQAGPYATQPRYDTAQLPALAANSYGAPA
                     VEVPSGPRAGAQGYHLSDEEPAMASRGTQWGAYGTQPLPPIE"
     gene            11601..12281
                     /locus_tag="VFB80_24565"
     CDS             11601..12281
                     /locus_tag="VFB80_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37036.1"
                     /translation="MRLVILALTLAGGIAVCGCSKSGSTPAKAAANVQPVKATPAASK
                     PVEVPANATPEQVVTVFLQALRSGDSPTTESLLTTKARTELGKHQMSVDVQAAPNAVY
                     QVEKATLVPNQPTIAHVNSLWTEKFENEQGRPAEEKYAIVWGLRRQTEGWRVAGMQVE
                     LVPGLRQYLDFEDPEDMMKKRDEALSAMQPPLAQAGATSPVEAPAIQTAQQPPVNPLQ
                     QPAPRVER"
     gene            complement(12640..13104)
                     /locus_tag="VFB80_24570"
     CDS             complement(12640..13104)
                     /locus_tag="VFB80_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37037.1"
                     /translation="MLLRLHRSLNGTRGLDGALHNHATAAAAVAAVATMATAMATMTA
                     AVAAVAARRAAARRAAAATMAAAVAAMAAVAAVAAAAAIAATTSEQQAEGRSLVFTAH
                     EGDTNQREKDRNTEHYNAVHPRFLQLLTGTGKREHKVAVITEPHLAARRPKR"
     gene            13337..14062
                     /locus_tag="VFB80_24575"
     CDS             13337..14062
                     /locus_tag="VFB80_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37038.1"
                     /translation="MSSSSDPTKDPPLDPTLRSVVSLLLVLHLFCVGVVLASNFRRSL
                     LQNELVRIFAPYTQLLDFDPQQTPYFYTFGRPIDDDAVFVLDLYAKGEEPAAGQPLVR
                     TVRLPAGGSNWFGDRQRYFQLARLLALYADPENENDDITSEIARSVGARIMRESASGR
                     AVLRVVRRDTQPPPTVPLAAGFPEDAPTDRAYDTVIYEADVWLDEDGAQVQRRSSAAE
                     VAPRQNTQRTSGTAAPGTNSAQP"
     gene            14059..15030
                     /locus_tag="VFB80_24580"
     CDS             14059..15030
                     /locus_tag="VFB80_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37039.1"
                     /translation="MTTTSDGYFSDVTAAVGRGWNRFWFTPADPLQAALLRIAIGLLA
                     IVHLVSLSQDLGRWYSREALLSPDAVKKLLAEGGETANYHYSYLGNFTDADIRYVHWA
                     AIAAAAAFTLGLVTPISGALTAAALLAYVHRVPQVAGHLEPVLVFLVLYLVVANSGAR
                     LSLDRRLFGRRQQPGGELPSTSIAANVGLRLIQVHLAMFYAMMALTKLYGDAWWDGNA
                     IWYLVARTHSRPVDFSWLRGWGRSGALLINFWTHAIVYYELAFAVLIWNRWARPLMLA
                     LGLIVWASLVVATGYLLFGLAMLLASAAFLPREFFAPVRQPQLSSPR"
     gene            complement(15038..15964)
                     /locus_tag="VFB80_24585"
     CDS             complement(15038..15964)
                     /locus_tag="VFB80_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN37040.1"
                     /translation="MIELIDFGKDYGDFRAVEALNLKIDAGEMFGFIGPNGAGKSTTI
                     RFLATLLKASRGEGIVNGHSVTQNPMAVRQSVGYMPDNFGVYDGMKVWEFLDFFAVAY
                     KIGKGKRKQVINDVLELLDLAHKRDDYVNGLSRGMKQRLCMAKTLVHDPPVLILDEPT
                     SGLDPRARIEVKALFKELKRMGKTILISSHILSELADCCTSIGIIERGQLLMHGPIDE
                     VYRRIRGNRTVEVRFVNGMDVGLSVIRSSPYVRNVAVDVSSCVLELEANDEQVNELLQ
                     QLIFNKCGVRSFSEKDPSLEDVFMMVTKGLVT"
     gene            complement(15995..>17562)
                     /locus_tag="VFB80_24590"
     CDS             complement(15995..>17562)
                     /locus_tag="VFB80_24590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37041.1"
                     /translation="TSFPLTDVRIKNWKNFDGFFNNVLLRRPPRKFSHNMGGSIQALN
                     VTWSEPGLATMLYEARIGSTLRYFTRDIGKPNADGTTPVNASGVTGAEDVATTPAAPP
                     YDPRAMMRRGFAGYEEAIPQRIDTSHSHPDLDDWHFAGYHSTSDSGVAAWNDKGAASE
                     AARAALTEAAGIEIPKAEFVMKVLGVYLLVLVPLNWLLFWLIGRVEWAWVAAPIIAIV
                     GAGAVIRLAQLDIGFARNRTEIAVLEMQGGYERAHLTRYTALYTSLTSNYTAAFAEPT
                     ALALPFPGGPRKESLLSIANYTDVIFRRDKETSLSGLLVYSNETGMLHSEQMLTLGKN
                     PKVTESLVLAGDEQKGYSIRNTTDITIRDVGIVRRVDPPGGNQPPRIETAYVAKLEPA
                     TSAPLRFSPLREDLVWLPEWNQSSSAFAPPAGEVERGRVRLTQLARLAAQRLRLLPGD
                     VRLVGWTSDLLPGMELRPAAPQNSTYTLVLSHLVRGPLPPVRADSNVFEDYLDPTFDL
                     EPATEGDMPETTP"
BASE COUNT         2992 a         5691 c         5826 g         3053 t
ORIGIN      
        1 ggccggagcg actcgtacat cacattcatc agatgccggt agtgcagcgg attctcgatg
       61 cggtagccga acgccagcgg accggccggc ccccagacgc ccgactgcgt gtggcagctc
      121 atgcagttgc tgccgtacat gctgccggtg tcgcggaggc ggcggtcgag gctgttgccc
      181 cgcggccggt tcatcagcca ggcagagacc tgggccacgt ggtcgtacat cgcgagctgc
      241 acggccgcgc gcgggtcggc cagcggcgcg ggtcggcgga tgcggatttc caggtcgtag
      301 ccgggcgagt tggcttccac ccgcagcaac agcacagcac ctggccgcag cgtgcggacg
      361 acggccgtgc ggtgctcctc gacctgctcg tgcgtcgtct cgttaaggtg cttgccttcg
      421 ctgtagtccg ctccgtccgg cgtgtagacc cgcacgcggg cggcgacaat ggggtcggtc
      481 ggcatcaggt tgacgctgag cagccgcggc gaatcgcccc ggtattccag gcggaaccag
      541 tcgtccccgg actcgccgta gcggctgttg tcaaaatact cgatgtcgtc cgcgccgccc
      601 gtgaggtgca gaatctggtc gccgtcgccg gctgccagcg gcagcggctg cgcttgggcc
      661 ggcgagttgt tcggctccag ctcgacgatg tgcccccgcg ttgccgtccc gaaatccacc
      721 ggttgcacca gcaggcgcaa ttcgaccttg tgcccggccg gccagggcgt ttgcttggga
      781 aacggtgcga cgtcggccag cacaccggtt tcgcgccacc gcggcgaatt ggcgggcggc
      841 tcttcgtcct cgaccgcgcg aagcgtcagc gtgtatttcc caccctgcgg cgcgcggtag
      901 atgagataga cgtcgggatc cagcgcgtga aggattttgc ggaagccgct gtccgcggcc
      961 ggcccgctcc actcgaccgc cacgcggccg ttggccggca gccgcgacgg cgcggcgatg
     1021 ccggccgaga tctcgaccgt ctggccggcg ctgagctcga tcgcttgcgc gtgagtgccc
     1081 gagaaagcga tccgctcatc gattttcgct ggctcatcag cgaccgccgg aagcgtccag
     1141 aaggccgccg ccaataccag cccaacgaag catggtttgc gcatcgccgg tgctcctgcg
     1201 gttgaagttg ccgcggggaa tcgccacagg gctcattatg ccgccgcgcc gctgcgcctg
     1261 gaaagtcaca agcgagaatt gtctcgcagc ggccagggtt ccataatccg tgggtcctta
     1321 aggaggtgcg ccatgtcaga ctcaacgacc cgccgcagtt tcctgatgca agccagccag
     1381 gctgcgctcg cgccggctat cgccacgtcg ctcgcccacc tcgcctcggt gctggcagcc
     1441 gctccgccgg gcggcgagga ctattggcag gtggtccgcc agcaattcga ctttcgcgac
     1501 gagaaggcgc cgatgaacgc ggccaacctt tgcccgtcgc cgcgagccgt cgcccagcgc
     1561 gtcaccgagt tgacccgcga catcgacgtc gattgctcgt tccagaatcg ggcgaagttt
     1621 gccggcctgt tggagacgtc gcggcagaaa gtcgccgagc agctgggcgt cacggccgac
     1681 gaaatcgccc tcgttcgcaa caccagcgag gccaacaaca ccatcaacaa cgggctgccg
     1741 ctcaagtccg gcgacgaagt cgtgctgtgg gaccagaacc atcccacgaa caacgtcgcc
     1801 tgggacgtgc gggccgcccg cttcgacctc cgcgttcggc ggatcactac tccggccgct
     1861 ccggccgacg cgggccagct cttgcaggcg ttcaccgatg ccttcggccc gcggacgcgg
     1921 gtgctggccc tcacgcacgt ctccaatgtc agcggcctgc ggctgccggt cgccgagctg
     1981 ggcgaagccg cccagcgccg cggcatctgg gtccacgttg acggcgcgca gagctggggc
     2041 gcgctgcacg tcaacgtgcg gaagctgaac tgcgactcct attcggccag cgcccacaag
     2101 tggttcgtcg ggccgaagga ggcgggcctg ctcttcgtcc gccaggagcg gatcgccgag
     2161 gtctggccca atgtcgtcgc gcccggctgg ggcaaccagg tgcagccgga agtcaagggc
     2221 gcccgcaagt tcgagtccct cgggcagcgc gacgacgccg cactggcggc catcgccacc
     2281 gccgccgagt ttcacgccgc catcggcccg gcgcaggtcg agtcgcggct gatagagctg
     2341 gccaccgcgc tcaagaccgg gctcaaggac gccggcatcg agctggtcac gccgctggag
     2401 ccgagcatga gcggcggcgt ctgcgtcgcc cgcgtgccgg cagccaagag caacgaaatc
     2461 tacaaccggc tgtatgagga gcacggcatc gccggggcca caaccggagg cctgcgcctc
     2521 tgcccgcacg tctataacac ccgcgagcac atcgaccgcg cgatccgcgg cgtgaaggcg
     2581 ctgctcagtt aggccaaaga atcgcgccag cggcgaaatt cctgctcgat ctcttcgcgc
     2641 gggcagcgct cgcgataggc gatgcgggcc cggtagccgt attcctggag cacgtcgtac
     2701 ttcggcacga tgaactgaaa gtcccaggcc gggttgggaa acgacggagc gccgccgctg
     2761 gagggcgagt gcgagaagcg aattccctcg ctgcggtcga acatcaggat gaaaatgtgc
     2821 gtcgtgccga tgtagccgta atagaacggc tcggcaaacc gcagcggcga gaggttcttg
     2881 tagagcgtct gctgcggcgt ttccaggaac ctcagctcca gcttgtcgtc cacgtgccgc
     2941 accgtgctct ggttgttgtg ctcctgcgtg cagagctgca cccagccctt cgaatcgcgg
     3001 aaatagatgc tcttgttctc cggggcgttg atgtagttcg cccagaacag gccgatgtag
     3061 ccgtagcgga aagcgtgctg gtgcggcttg cagcgaaacg tgaaatcgac gtagtgcggc
     3121 ggcacgagct tgaaccggct ccagctctcc aggtggtagg tcggcgtcgg cgtctggtgc
     3181 agctcggcct cgtcggcggc gagccgccgg tactccatcg gatggttccg cggctcgaag
     3241 aagatcttcc ggtcgccgga gaggttcagc tggtccttct cgccgtcgaa gatgtgttcg
     3301 aagttcagcc cggccacgcc cagcaaagac ggctccggcc gcgtccggtg aaccagccgg
     3361 tgaattccgc tatacccgta ccggcgatcg tcgtaggcct cgttgtcgcc gatgaccgcg
     3421 gtcaggtcgc cgacctgaaa agtctcgtgg gccatgtgcg tctcctgcgg ctaacgtgac
     3481 tgctccccgg ccggactgcc ggcggccaga cgccgataga agtccagcgc ctgcgagaac
     3541 tgctgctcga cctgcgtctg ctgggcggcc gggatgcgat ttcgctggcg gacatcggcc
     3601 cgcaggcgct cgatccagcg gatgaaatac tccgcgtcct cggtcgagcg gacggggcgg
     3661 ccggcgactt ccacatagac cgcgctggtg tgggcaaaca gctccgcggc ttgcattccc
     3721 ggcggacgcc gcccccgcac gcggaaggca atccagccgc tcgtgtcgag cggcacttcc
     3781 tggttcaacg tgagctgctc gggcttttcc ccagcggcgg ctgtcgcagc gacctgccca
     3841 ttgaccagga cttccagctt ttccagcgga aactgcgagc tggcttcgca cgcgaccgcg
     3901 gccttgccgg gcccagccag cttgatcgtg ttgcccgctt cctgaccatt cacggtgaag
     3961 cggagcatcg gcccgttggt gacaaacgtc tgccccgccc gcagccgggc gatccaatcc
     4021 tgatacgtaa gtttctccgg gcagtgcaca taaacccggt cggagccggg caatcggctg
     4081 acgatccggt tcaggaagca atcggtgccg gccgacgccg gaacgcgcag cccgcagttc
     4141 agcagccgat accagaccgg caggttggcc tggtgattcg agcccatcac gtcgatcgaa
     4201 tcgactttcc ccagggcgat gtcgatcggc atctccttgg cggagtaggc gctgtcgtag
     4261 gggtcctgga ggttgttggc cgggtgcgtg tagttgacgt ggcccccctg ctcgtgcgtg
     4321 tggtccgcga tgtcggcatt cgtcggcacg tcgtgcgggt gcgtcgtgtg gcggaacccc
     4381 gtgaagatcg gcacgaccaa ccgcttcaag ttcagcagcg tcatgtggcc ccagatcgtg
     4441 ctgcggaact cctcgttcca gtagaggatc gtccgctcgg tcgaaagcgg atcggggcgg
     4501 ccgaggaaaa actcgcggtc gaacacgccg tcgccgtcgg agttggcaac catgaagttg
     4561 gccacggtca ggtcctcgcc ctcgcactgc aggcgcatcg tggccggcga gttgtaccag
     4621 tggccgtagc cgtaattggc gtggatatgg ttctcgcctg agacccagcc ggcggagcgc
     4681 tggttagtcc agcgctcgat ccgaagcgta accttggccg actcgccctg gcggacggtg
     4741 aacgtctgcc gcacggagcg atgctccggc ccgcgggccg ccgcaacggt gtactcgccg
     4801 atcggcagct ccatccgcac gtgatcctgc agatagaagt gcatgtcgcc gccgagcacg
     4861 cggtagagcg cgccgctggg ggcgtgatgc ttgccgtcgg catggcggag gacgatccgc
     4921 gccgtcgtgg gcgagtgctc cgcgccttcg gtcactttca attccaccgc gcccttgggc
     4981 gtgccaaaat cgcgctggct cggcgcgacg atctgctcct gcccgctggc caggtcgcga
     5041 agcgccagca gcgtcggccc cagacggttg tcggtgaaca gcagccaccg gccgtcgtcc
     5101 gatacgctcg ggctgctttc gtcggcctgg ccgaaagtca gcttgcgtgc cgcggcgtcc
     5161 gcgccggcca gcggcacttc ccacaggtcg tggtggacgc tgccgcgctc ggtcgcgatg
     5221 aacagggcct ggtcgtcggc acgaaacgcc agcccgtgaa tccgcgccgg ccagtggacg
     5281 atcagctccg gcttgccgcc gcccagcggc acgcgccaca ggtccgtctg gggcccgcgg
     5341 tttcccgatt gttcccccgg ctcgtccgct gccgagacga cggccagagc ggcgttatcg
     5401 tgcgacaggg caaacggctg ccgcgagatg cccggcgtgc cgggcggctg tccgggccac
     5461 gactcggcag cgactgcggc cgtcaattct cccgattgca gatcgaacca ggccagccgc
     5521 acgcgctcgc ctgccggctg aaagagaccc agcacgcggc gaccgtcgcg gctgaagtgc
     5581 agccgatcga tggcggtcac ctcgcgcggc agtttcacat tctcgccggt gcgggcatcg
     5641 atcaccgtca gcgtgccgct gccgaaagtc gagccggata tcagcgctag tcgcgtgccg
     5701 tcgggcgccc aggccggctc gacgtcgaaa ccgccgccgc gggtcagccg ctgcatcgtg
     5761 ccgcccgccc gcggcatgcg ccagatcgcg ccctgatagg agaacgccag ttcctcgccg
     5821 ccgggcgcta gccgcggcat gatcgcgccg aaggcccgcg gatcgacggg ctccgactcg
     5881 tgtgcgctgg cccaggcagt tccgagcacg acgactgcga acgacaacgc acgaatggcg
     5941 cgggtcatgg ccgtgtccct cgaaaaaccg cgttcccacc tgcgtgcggc attgtagcag
     6001 cgcgagcgcg ccgcgacgcg gatagaatga ccgtatggcg cgcgtgaacc agcagcggtg
     6061 gcaatacacg ctggccagcc tgttctggct gatgctcctg gtcgccctgc tcgccggggc
     6121 ctggcggatc gggtggatga aagtcgccgc gacagtcgcc atcggcaccg gcgttttgtt
     6181 ctgcctggtc tgggccgcct tcttgttcga cgagctccgg cgatgagcga taatccttac
     6241 gaatctccgc agaccgagcc gcggcgtaga ccgcggccgc ccatcctggc gggagtggtg
     6301 ttgtttgccg ggctggctgt tgtggccttg ttgatcgccg tcgcggtcgt ctgtgtggcc
     6361 tgaactgaag cgtgactgtg ctagcgccgc acagtgtctc gacgcgcggt gagtggccag
     6421 cgcggaggag ttgccctcaa gcgtcatctt tccgccatcc gaaagcacat cgtaaaccgc
     6481 agcgttgagc gaagctgcag gagtggtgtc aagctagcga tctactgcaa ttaccgcagc
     6541 ggcgcgctgt tgcccgtcgc tggggatgac aaggagggac ggcgaatggc gatctcctcg
     6601 gcttatcaga cgctgcggac gagctggcgc ggtgtgcggc ggtttgtaaa gaaagtccct
     6661 tacttgggat ttggacgctg gtgttcggtt tgcggccgcc gatcgcgaag gttttgccga
     6721 caccgccact tcccgaaccc cgaggcgaag tgcgtccact gtggggcctt tgcgcggcac
     6781 cgcttcttct ggctgtatgt caacgcccac acggacctgt tcgacggccg gccgaagcga
     6841 atgctgcacg tcgcgcccga ggcctgcttc atcgacaaga tgcgggagcg gctcggcgag
     6901 ggttacgtga cggccgattt ggacgacgag cgggcgatgg tcaagatgga catcaccgcg
     6961 atccaattcc ccaacgcctc gttcgatgtc atctactgca gccacgtgct ggagcacgtg
     7021 gacgacgacc gccgggcgat gcgcgagttc taccgcgtcc taaagcccgg cgggtgggcc
     7081 atcctcctcg ttcccatcga cgccgcgaaa acgtttgagg acccgtccat cgtcgatccg
     7141 gccgaacggc tgcggctgtt cgggagcgaa ttccacgtcc gccgctacgg gcccgactac
     7201 gtcgatcgcc tccaagaggc gggcttcgag gtgcaagaga cccgcatcgc cgacatgttc
     7261 agcgacgatg aggccctgcg gctggggatc gcccgaggcg cgggcgacat ccatcactgc
     7321 accaaatgag atcacctccg cctggcgcga atgacactcg gggcggccgc ccgtgaatca
     7381 agtctcacac agaggcacag agacacaggg cagagcggag atcaggtgct gcggtttcca
     7441 ggtcggacga cggatgccac agcacagcga aaaaactcaa agttattctc tgtgtctccg
     7501 tgtctctgtg tgagctttct gctctcttcg tgcaatgaac gctcaaatta acatccctgc
     7561 gctgggtcgt accccaactg cttgcccagc cgctcgtagc tgattccgac ggtgagatcg
     7621 taggccacgt gaacggccat cgccacatac agcgagccgg cgagccagac cagcaggtgg
     7681 aagccgaggg cgaagaccac gatgagggca gcggacttcc agccctggac gatgtggacc
     7741 aggccaaagg tgatggagca caccagcgag gctgcccaaa agctgccggt gagcgcgccc
     7801 actagcgcgg cttgggtgcc ccgccaggtg atttcctcgc cgacgcccgc gatcacggcg
     7861 acaattatcc accatatccg ctccctcatg tttgacggca tgaagagatg cacgacgcgg
     7921 gctcggcgct cgaccgcgcg gcgccaattc ggccgcatgt aggcgacggc cgccgcatag
     7981 gcgaccacgc cggcaatcac ggcgcccaga ggcgggaaag ccaacgggaa cagttcgatt
     8041 cgctgcaccc gcgcgacgag cagcgagcag atcgcaagca gccccacttc cagcgccgtc
     8101 cgctggaagt ggcgtaaccg atccggcaat gctcccccgg agcccaacac cttcttccga
     8161 ttgcgggccg ccatgatcgg caggtaaacg ccaaagaatc cgagatgaaa ccagcctgcc
     8221 agattgagat aggcggcgga cggaaacatg cttggtgctt ggctgggcga ccaaaatggg
     8281 tgcgggttac ttgctcaggc ctaatctacc cgccgctgac tacggaagtt gcgatttcgc
     8341 tgatgaaacc gcgccagccc acacttgact gctttgcgca cttgcggctt ggggtcgaaa
     8401 agatggcggc gaacggcgtc gagcgtatcg tcgtcgccga tggagcccaa ggcgtaggcc
     8461 gccgcttggc gcagtcgcca gttgcggttt tccaaggcca agaggagagg cgcgacggct
     8521 tgacgatctt cgatccaacc aagcgcgccg gcagccgcgg ccgctacctc gatgtcatcg
     8581 tcctttagat gccgaatgag gggtccgacc gccttccgat cccgcagcat ccacaatccg
     8641 ccgattgcgg ccttgcgaac ctcggaatag ggactctcca gcgcctcgag aagcgaaggt
     8701 agggacgatg gctcgttaag caagcgcagg ccggtaatgg cctggacttg cgcgtcgcgg
     8761 tcccctgttt tcagaacggc aatcaagggc tcaacggcga agcggccgat ccccactaag
     8821 gtagctgcgg ctcgccgcga gaaggccgac ggcgtgttgc aaacccgact gagggctacg
     8881 gccgccgcgg cgccgctccg cagcaattgg ccagccgcag ccgcgtggac agacacagcg
     8941 tcggaggcca attgcccgat caggtcttga atgcgattgg acataccacc gatcaaacct
     9001 cacacagagc cacaggggca cggagcagag accgaagtta cctccgtggc cgccgcgtct
     9061 ctgcgtgagc ccgcgccgat gcgaactgtg aacattggta cggtatttgc attgaagttc
     9121 cgaatgacct gccgtctgcc attttggcaa cggcgatggt ggtcgatgcc agatcgccct
     9181 ttcgtcaaag atgcaacaaa gacccatgag ggtttaagac aaaatgtctc taaaccttct
     9241 aatgctttta aggctaatgc tgcaggggcg cctgatcgtc aatgcaactc attgtcgcta
     9301 aacgacttgc gaacttggcc tcctttgcaa gggcgcgacg ggccggcatt aatccaacgg
     9361 ggttccgctt gtgccgatgg taacggacct gcggcgaacg agaggccggg tcgattgctt
     9421 cgcgatagca ggccgatgac ccgtgaagcg taggctatcc tgcgctccca cccgaattgc
     9481 ctgcccgaga aacggaattt cccgcctcct cccaagcatg gagtgctgtc atgcgtcgtt
     9541 ggctgtgggc ctgctcggcc tcgattggtt tggtcctcgc tgcgaactct gcccaggccg
     9601 actatggcac cgggtggggc caccgcttcc acgtcgactt ctaccgcaat aacacctggc
     9661 cggagccgtt ccgcaccgcg gaccggaacc tggcccgcga ggcgttctgc atccaggtcg
     9721 ataacggttg gaagatgcag aacacggtcg gctcgctgct cttcaacccg gagacgcagc
     9781 agctgaacca ggccggcgac ctgctggtga aatggatcgt aacgcaggct ccagtccacc
     9841 gccgggccgt gttcgtggtg aagggcgaca acgccgacat cacgcgggcc cgcgtcgatt
     9901 cggtcaacgc tgccgtcgcc cgctacgctc aaggcccggt gtgcccggtt atgctgacca
     9961 cggtcgagcc cgaaggctgg ccggcgtcat acatcgacag catcacccag cagttcaact
    10021 cgtcaattcc ttcgccgcgc ttgccaggcg gcggcggcgg tggcggcggc ggtggtagtg
    10081 gcggcggtgg cggcggcaac ggcggcggtg gcggcaacgg cggcgggtcc ggcggatcca
    10141 gcccgtagcc gagctagcac gccggccaaa ggccggttgc ccgcaggact tgccccggcg
    10201 gaatggtccg ccggggttgc gccacgcgaa aattgggctg gaggttaaag ccagccccag
    10261 acgatgtgtt gagagggagc cgcgcgaagc gccgcaggcc gcgcttcgca agctccgcta
    10321 gggaatgtca gccgtcacgg atggacggcc cgcggaacac ggaggtcaca gtgaaaatcc
    10381 agcgcaagct cgcggtcacg atgctcttgg gagcgggggt cggcctgtgc ggctgccagt
    10441 cgccctctca aggcggcctc gcgttctgga agaaagacag caatagctcg ctggcgtcgg
    10501 ccactcccga cgtcggcgca cagcgttatc aaggcctggc caaggagttt ggaagccaat
    10561 cgacgggcgg cgcgacggcc ttgggagcgc agaagccggc gcccgacaac ttcctgatgg
    10621 cctcgtggaa gaaaacgaca ggcgccgttg ccggcgcgtt cgccgcgaag cccagcacca
    10681 cggaagcgag cgatccactg cggctcgatc agccgacgaa gaagctcggg ccggaagtcc
    10741 acatgggcgc ggcccaactg atggagaatc aagggaagtt ccctgaggcg gaagagcatt
    10801 atcagaaagc gctgaagcag tcgccgaata acctgggcgc gctcgtcggg ctggcccggc
    10861 tgcacgatcg gcaggggcac tcgcgggcgg cgctggagac ttacgaccgg gccctcaagg
    10921 cccatccgca gagcggcctg gtgctcaacg acctgggctt gtgcctggcg cggcagaagc
    10981 aattcgatcc ggcgctgggc gcgctgaaac gagcagtcca gaacaacccg gagaatccga
    11041 aataccgcaa caacctggcg acggtgttgg ttgagacggg ccgtgtcgcc gaagccctgc
    11101 agacgctgtc ggccggttcg tcgccggcgg tcgcgcacta caacgtgggt tacctgctcg
    11161 cccagcgcgg ccagacgaac gaagccgccc agtattttca gcaggccctg gcgatcgatc
    11221 cggcgctgac ccaggcccgc gacatgctcg cccagctggc cggcgccggc ggcacgcagc
    11281 ccatgccgca agccgggccc tatgccaccc agccgcggta cgacacggct caactgccgg
    11341 cgctggccgc gaattcgtac ggcgcgcctg ctgtcgaggt gccgtccggg ccacgggcag
    11401 gggctcaggg ctatcacctg agcgacgaag agcccgcgat ggcctcgcgc ggcacacagt
    11461 ggggcgctta cgggacgcaa ccgctgccgc cgatcgagtg acgctagcag ccggtattcg
    11521 ccttgaccgc cctcggggtt cctgcgacat ttcccgcccg caaggacggg gagcgcgaac
    11581 cagcgagaag gagctcggcg atgcgtctag tgatcttggc cctgactctg gccggtggaa
    11641 tcgcagtctg cggctgctcg aagtcgggaa gcaccccggc gaaggcggct gccaatgttc
    11701 agccggtgaa ggccacgccc gccgccagca aaccggttga ggtcccggcg aatgcgactc
    11761 cggagcaggt cgtcacggtt ttcctgcaag cgctgcggtc gggcgattcg ccgacaaccg
    11821 agtcgctgct gacgaccaag gcccgcacag aactgggcaa gcaccagatg agcgtcgatg
    11881 tgcaggcagc gccgaacgcg gtgtaccagg tggagaaggc gacgctggtg ccgaatcaac
    11941 cgactatcgc acacgtcaac agtctctgga cggaaaagtt cgagaacgag cagggccggc
    12001 ccgcggaaga gaaatacgcc atcgtgtggg gcttgcggcg acagaccgaa ggctggcgcg
    12061 tggccggcat gcaggtggag ctggtgccgg ggctccggca gtacctcgac ttcgaggacc
    12121 cggaggacat gatgaaaaag cgcgacgaag ccctgtcagc catgcagccg ccgctagcac
    12181 aagcgggcgc cacttctccg gttgaagctc ccgcgattca gacggcgcag cagccgccgg
    12241 ttaacccgct ccagcagccc gcgccaaggg ttgaacgcta agtacggcat agttcgcagg
    12301 cttggcacag ttttacacca tagatgcaac ttagtttcgg taaatgactt aggtcacgtc
    12361 gcgctgggcc ggacggaagt tcccggcccg atcttttttg ccagttctac tggacaggcg
    12421 caaagcgcgt cgtaaatttt cccgtgcttg gcatttctta cgccaagcac cggatgcacc
    12481 caaactgtgc attttaactc ccaccgcttg acatggggtg gggctcgact accctacgac
    12541 ctagcctgtg agatctctat atccttcgta acccgcagac tttcacaaca gggagtggcg
    12601 gcttttgggg gagcagttat tcgcaggtca ggccgcatct caacgtttcg gccgtcgggc
    12661 agcgagatga ggctcagtga tgacggcaac tttgtgttcc cgcttaccgg tacctgtaag
    12721 taactggagg aatcgaggat gaaccgcatt gtagtgctcg gtgttgcgat ctttttcgcg
    12781 ctggttggta tcgccctcgt gggcggtgaa aacaaggctt cggccttcgg cttgttgttc
    12841 cgaggtggtg gcggctatag ctgcggcggc tgcaacggct gccacggcgg ccatcgctgc
    12901 cacggccgct gccatggtgg cggctgctgc tcttcgagct gctgctcttc gagctgccac
    12961 ggctgccacg gcggccgtca tcgttgccat ggccgttgcc atggttgcaa cggctgcaac
    13021 ggcggctgct gcggtggcgt ggttgtgaag ggcgccgtcc aggccccggg tgccgttcag
    13081 gctcctgtgc aggcgaagta acatcgccag tcccgctgcc cgccggccgc tccccctgtt
    13141 ggcgccggca agcggaagcg attcaccgaa agatcgggct ctccccagag tccgatcttt
    13201 cgtcgtttct tggccggaac tgcggcaaag ccgcttgtga cgacttgacg gcttgcgttg
    13261 gcccgccgga tgctgctggc tcaggcgagc ttcgccggcc gatgacagcg aaggcttccg
    13321 cacttcgggc ccggtcatgt cgtcctcctc cgatccaacg aaagacccgc ctctggaccc
    13381 gacgctgcgg agcgtcgttt cgctattgct ggtgctgcac ctcttttgtg tcggcgtcgt
    13441 attggcgtcg aacttccgca ggtcgctgct gcaaaacgag ctcgtgcgga tcttcgctcc
    13501 atatacccag ctgctcgact tcgatccgca gcaaacgcct tacttctaca cgttcggacg
    13561 gccgatcgac gacgacgcgg tcttcgtcct cgatctctac gccaaggggg aggagccggc
    13621 ggccggtcag ccgctagtca ggaccgttcg cctgcccgca ggcggctcga actggttcgg
    13681 cgaccgccag cggtactttc agctggcccg gctgctggcc ctgtacgccg accctgagaa
    13741 tgagaacgac gacatcacga gcgaaatcgc ccgctccgtt ggtgcacgga tcatgcgcga
    13801 gtcggctagc ggccgggccg tgctgcgggt cgtccgccgc gatactcagc ctccgccgac
    13861 ggtcccgctg gccgccggct tcccggaaga cgcgccgacc gatcgggcct acgacacagt
    13921 catctatgaa gccgacgtct ggctcgacga agacggggcg caggtccagc ggcggtcctc
    13981 ggccgccgaa gtcgcgcctc ggcaaaacac gcagcgtacg agcgggactg ccgctccagg
    14041 aacgaactcg gctcagccat gaccacgacc tccgacggct atttcagcga cgtgaccgcc
    14101 gccgtcggcc gcggctggaa caggttctgg ttcacgccgg ccgatccgct ccaggccgcg
    14161 ctgctgagga tcgctatcgg attgctcgcg atcgtgcacc tcgtcagcct gagccaggac
    14221 ctgggccgct ggtacagccg cgaggccctg ctctcgcccg acgcggtcaa aaagctgctc
    14281 gccgagggcg gcgagacggc gaactaccac tattcctatc tcggcaattt caccgacgct
    14341 gatatccggt acgtgcactg ggcggcgatc gccgcagccg cggccttcac gctggggctg
    14401 gtcacgccga tttccggcgc gctcactgcg gcggcgctgc tggcctatgt gcaccgcgtg
    14461 ccgcaggtgg cagggcacct ggagcctgtc ctcgtctttc tcgtgctata tctcgtcgtc
    14521 gcaaactccg gtgcacggct ttcgctcgac cgccggctgt ttggtcgcag gcagcagccg
    14581 ggcggcgaat tgccatcaac cagcatcgcg gcgaacgtcg gcctgcgatt gatccaggtc
    14641 cacctggcga tgttctacgc catgatggct ctgacgaagc tctacggcga cgcctggtgg
    14701 gacggcaacg ccatctggta tctggtggcc cgcacgcact cgcggccggt ggatttctcc
    14761 tggctccgcg gctgggggcg cagcggagca ctgctcatca acttctggac gcacgccatt
    14821 gtgtattacg agctggcgtt tgcggtgctg atctggaacc gctgggcccg gccgctgatg
    14881 ctggcgctag gcctgatcgt gtgggcctcg ctcgtcgtcg cgaccggata cctgctgttc
    14941 ggtctggcga tgctgctggc cagcgccgcg ttcttgccgc gggagttctt cgctccggtc
    15001 cgccagccgc aactctcttc gccgcgctag ctgagcttca agtcaccagg cccttagtga
    15061 ccatcatgaa gacgtcttcg agcgacgggt ctttctcgga gaagctgcgg acgccgcact
    15121 tgttgaagat caactgctgg agcagctcgt tcacctgctc gtcgttggct tccagttcca
    15181 ggacgcagct ggagacatcg accgcgacgt tgcggacgta ggggctggag cggatgaccg
    15241 agaggccgac gtccatcccg ttgacgaaac ggacttcgac cgtgcggttg ccgcggatgc
    15301 ggcgatagac ctcgtcgatc ggcccgtgca tcagcagctg cccgcgctcg atgatcccga
    15361 tcgatgtgca gcagtcggcc agctccgaca ggatgtggct gctgatcagg atcgtcttgc
    15421 ccatccgctt gagctctttg aacagggcct tgacctcgat gcgggcccgc gggtccaggc
    15481 cgctggtcgg ttcgtcgagg atcagcaccg gcgggtcgtg gaccagcgtt ttggccatgc
    15541 agaggcgctg cttcatgccg cgcgacaggc cgttgacgta gtcgtcgcgt ttgtgggcca
    15601 agtcgagcag ctccagcacg tcgttgatga cctgcttccg tttccccttg ccgatcttgt
    15661 aggccaccgc gaagaagtcg aggaactccc agaccttcat gccgtcgtag acgccgaagt
    15721 tgtcgggcat gtagcccacg ctctggcgta cggccatcgg gttctgcgtc acgctgtggc
    15781 cgttgacgat cccctcgccg cggctggcct tgagcagcgt cgccaggaac cggatcgtcg
    15841 tgctctttcc ggcgccgttg gggccgatga agccgaacat ctcgcccgcg tcgatcttga
    15901 ggttcagcgc ctcgactgcg cgaaagtcgc cgtagtcttt gccgaaatcg atcagttcaa
    15961 tcatgggcca ggagtcggga gtcgggtgtc ggggtcaggg agtcgtttcc ggcatgtcgc
    16021 cttcggtagc gggttccagg tcgaacgtgg ggtcgaggta gtcttcgaac acgttcgagt
    16081 cggcgcggac cggcgggagc ggcccacgga cgaggtgcga caggacgagc gtataggtcg
    16141 aattctgcgg agcggcgggg cgcagctcca tgccgggcag caggtccgaa gtccagccga
    16201 ccaggcggac gtcgccgggc aagagccgca gccgctgggc cgccaggcgg gccaactgtg
    16261 tcagccgcac gcggccccgc tccacctcgc cggcgggcgg agcgaaggcg gacgacgact
    16321 gattccactc ggggagccag acgaggtctt cgcggagcgg gctgaaccgc agcggggcgc
    16381 tggtggccgg ctcgagcttc gcgacgtaag ccgtctcgat ccgcgggggc tggttcccgc
    16441 cgggcggatc gacgcgacgg acgatcccca cgtcgcggat cgtgatgtcg gtggtgttgc
    16501 ggatggaata gcccttttgc tcatcgccgg ccaggacgag cgactcggtc accttgggat
    16561 tcttgcccag cgtgagcatc tgctcgctgt ggagcatacc cgtctcgttc gaatagacca
    16621 gcagtccgct gagcgaggtc tccttgtcgc ggcggaatat gacgtcggtg tagttggcga
    16681 tcgagagcag cgactccttg cgcggaccgc cggggaacgg aagcgcgagg gccgttggct
    16741 cggcgaatgc ggccgtgtag ttgctggtca gcgaagtgta gagggcggtg tagcgcgtga
    16801 gatgggcccg ctcgtagccc ccctgcattt cgagcacggc gatctccgtg cgattgcggg
    16861 caaagccgat gtccagttgg gccaggcgaa tgaccgcgcc ggcgccgacg atggcgatga
    16921 tcggggcggc aacccaggcc cattcgacgc gcccaatcag ccagaagagc agccagttga
    16981 gcggcaccag caccagcagg taaacgccga gcactttcat cacgaactcg gccttgggaa
    17041 tctcgatgcc ggccgcttcg gtcagggcgg cgcgggctgc ttccgaggcc gcgcccttgt
    17101 cgttccaggc tgccacgccg gaatcgctgg tcgagtggta gccggcaaaa tgccagtcgt
    17161 cgagatcggg gtggctgtgc gacgtatcga tccgctgagg aatggcttcc tcgtatccgg
    17221 cgaacccgcg tcgcatcatc gcccgcggat cgtagggagg tgcagctggc gtagtcgcaa
    17281 catcttcagc gcccgtgacg ccggacgcgt tcaccggcgt cgtaccgtcg gcgttcggct
    17341 tgccgatgtc gcgggtgaaa tagcggagcg tcgaaccgat tcgggcctcg tacagcatcg
    17401 tcgccagccc cggctcgctc caggtcacgt tcagagcctg gatgctgccg cccatgttgt
    17461 gcgagaattt tcgcggcggg cggcgcagca gcacgttgtt gaagaagccg tcaaagttct
    17521 tccagttctt gatccgcacg tcggtcagcg gaaagctcgt ga
//
LOCUS       DASRJB010000929         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3657106, whole genome shotgun sequence.
ACCESSION   DASRJB010000929 DASRJB010000000
VERSION     DASRJB010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657106"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..459)
                     /locus_tag="VFB80_24595"
     CDS             complement(1..459)
                     /locus_tag="VFB80_24595"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN37042.1"
                     /translation="MRNIRVAAVQLQHAPGDKAANLAAIERFASQAAAKRAQIIAFPE
                     CCISGYWHLRKLTRPQMEALAERVPDGAAAQRLLALSKQHGMTIGAGLLEIDSSGKLF
                     NSFFVAMPDGRWAWFTISMSCTIVCSPSPTTCPGVRRVAATSSPLTTSSR"
     gene            704..2020
                     /locus_tag="VFB80_24600"
     CDS             704..2020
                     /locus_tag="VFB80_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN37043.1"
                     /translation="MIRRRQFLQHSAAVIASGYFVSGLAAQESKSANEKLNLAIVGTA
                     NKGWHNVQNLTSQNMVALCDVDANYLAHAAMSFPLATQYRDYRKLLDAETKRIDAVVV
                     STSDHMHAPITSAALDLGKHVYCEKPLTHTVKEARTVAELAKRKKLATQMGTQIHAED
                     NYRRVVELVQSGAIGRVTLVHNWCNKGWSGGKFEPWDKPTPAHLDWDLWLGCAKERPY
                     SPNIHPNSWRRFYEFGGGTFGDMACHVMDLPFWALGLTHPTSVVCEGPPVDPVSTPAW
                     SKATFEFPAVGQRPAITMTWSDGGEHFDLVKSTKDHAGKSLSTWGLGILFVGDKGMLA
                     ANYTRRQLLPQDRFADHSPPPQTIEKSVGHWQEWVNACKSGSPTTCNFEYSGRLTETV
                     LLGIVAYRSGRKLEWDAASLKAKNSPEADQFITKHYRQGFEVVGIS"
     gene            complement(2175..2639)
                     /locus_tag="VFB80_24605"
     CDS             complement(2175..2639)
                     /locus_tag="VFB80_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37044.1"
                     /translation="MYPLTVCFPQPLCVLPALDIGPRTDGFADVVYSALDALDCGRAA
                     GERRREARSPYPYPIHLTPYDHHGEPDPRRTFVVIGKHLSHHGLDFYCRQALPDKRVV
                     ASLDCGKNGWIGLVLELTWCRFGRHGWYDNGGRFVAEVRSPLLDLDEQPRVA"
BASE COUNT          550 a          914 c          924 g          476 t
ORIGIN      
        1 tcatcggctg ctcgtcgtca acggcgagct ggtcgcagcg acgcggcgca cgccggggca
       61 cgtcgtcggc gacggcgagc acacgatcgt gcagctcatc gagatcgtga accaggccca
      121 gcggccgtcg ggcatcgcca cgaaaaagct gttgaagagt ttgcccgagg aatcgatttc
      181 caatagccca gcgccgatcg tcatcccgtg ctgcttcgaa agcgcgagca gccgctgggc
      241 cgccgcccca tccggcaccc gttcggccag tgcttccatc tgcggccgcg tgagctttcg
      301 caagtgccag tagccggaga tgcagcattc gggaaacgcg atgatttggg cgcgctttgc
      361 cgccgcctgg ctcgcgaagc gctcgatcgc cgcgaggttg gcggctttgt cgcccggggc
      421 atgctgcaac tgcacggcgg cgacgcggat gttgcgcatg gctcgttcct tcaataactt
      481 cgcagccccg atcaattgag cagatatcgc tcgtttagcc aaggaaccag aagcaaccgc
      541 ggattgcgcg gatgacgcgg atgaaatcaa ttcagcttct cccatatccg cgccatctgc
      601 gccatccgcg gttcatctgc tcttgatcca tgtcttggtg cgactcgatt ggctagactg
      661 gtcgcgtgac ctccaattcg cattgcaagc taaggagcca gtcatgatcc gtcgccgtca
      721 attcttgcag cattccgcgg cggtgattgc gtcgggctat ttcgtcagcg gcctggcggc
      781 gcaggaatcg aagtccgcca atgaaaagct gaacctggcc atcgtgggga cggcgaacaa
      841 gggctggcac aacgtgcaga acctcacgag ccagaacatg gtggccctgt gcgacgtgga
      901 cgccaattac ctcgcgcacg ccgcgatgag ctttccgctg gccacgcagt atcgcgacta
      961 ccgcaagctg ctggacgccg aaacgaaacg catcgacgcg gtcgtggtct ccacgtccga
     1021 ccacatgcac gcgccgatca cctcggcggc gctcgacctg ggcaagcatg tctattgcga
     1081 gaagccgctg actcatacgg tcaaggaagc ccgcacggtc gccgagctgg ccaagcggaa
     1141 gaagctcgcc acgcaaatgg gtacgcagat tcacgccgag gacaactacc gccgcgtggt
     1201 cgagctggtg caaagcggag cgatcggccg cgtcaccttg gtccacaact ggtgcaacaa
     1261 aggctggtcg ggcggcaagt tcgagccctg ggacaagccc acgccggcgc acctcgactg
     1321 ggacctgtgg ctgggctgcg cgaaggagcg gccctattcg cccaacatcc atcccaacag
     1381 ctggcggcgg ttctatgaat ttggcggcgg caccttcggc gacatggcct gccacgtgat
     1441 ggacctgcct ttctgggccc tgggtctgac tcatccgacc tcggttgttt gcgaagggcc
     1501 gcccgtcgat ccggtcagca cgccggcctg gagcaaggcc acgttcgagt ttcccgcggt
     1561 gggccagcgg cccgcgatca ccatgacctg gtccgacggc ggcgagcact tcgacctcgt
     1621 gaaatcgacg aaggaccacg ccggcaaatc gctcagcacg tggggcctgg gcatcctgtt
     1681 cgtcggcgac aagggaatgc tcgcggccaa ctacacccgc cgccagctgc tgccgcagga
     1741 ccgcttcgcg gaccattcgc cgccgccgca gacgatcgaa aagtcggtcg gccactggca
     1801 ggagtgggtg aacgcctgca agtccggctc gccgacgacc tgcaacttcg aatactccgg
     1861 ccgcctgacc gagaccgtgc tgctgggcat cgtcgcctat cgcagcgggc ggaagcttga
     1921 gtgggacgcg gccagcctca aagccaagaa ctcgcccgag gccgatcagt tcatcaccaa
     1981 gcactaccgc cagggctttg aagtcgtcgg tatctcgtag ggtgtcgcta ctccgccgca
     2041 ggcataggcg actcgaagtg gattttggag gagggtgtga atggccgcgg atgaacgcgg
     2101 ataacacgga tcggaaatag tttgtctgat ccgcgtcatc cgcgttcatc cgcggccata
     2161 tttcctcaaa atcctcaagc cactcgcggc tgctcatcca aatccaacag cggcgaacgg
     2221 acttcggcca cgaaccggcc gccgttgtcg taccagccgt gtcggccgaa acggcaccag
     2281 gtcagctcca gcaccaggcc gatccagccg ttcttgccgc agtcgagcga ggccacgacg
     2341 cgtttgtcgg ggagtgcctg gcggcagtag aagtcgagcc cgtggtgcga gaggtgcttg
     2401 ccgatgacga cgaacgtgcg ccgcgggtcc ggctccccgt ggtggtcgta gggcgtgagg
     2461 tgaatcgggt agggataggg actgcgggcc tcgcggcggc gttcgcccgc ggcccgcccg
     2521 caatcgaggg cgtccagggc ggagtaaacc acgtcggcaa agccatcggt tcgcgggccg
     2581 atatccaggg ccggcagcac gcagagcggc tgcggaaagc agacggtcag tgggtacata
     2641 gcgccggtgg gaaagtacgt ccaggcattc ttgacgatta tcgaaaccgg acgctgcgag
     2701 agaatcccgc gcgcggcgaa gcggaaaatc aggcggggag gccggttggg aagtccggcc
     2761 gccccgcgtg cggttcaagg aaacgacccc taacccctcc gctccccggc acaaagacgc
     2821 gccggacgga aagtcgggat cggttaaggc cccgctcaag cagg
//
LOCUS       DASRJB010000930         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2247364, whole genome shotgun sequence.
ACCESSION   DASRJB010000930 DASRJB010000000
VERSION     DASRJB010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247364"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFB80_24610"
     CDS             complement(<1..687)
                     /locus_tag="VFB80_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZN37045.1"
                     /translation="MKQTRYDWAAKGDVNAFFGLMLDNVANLLLTVSLLSGVFAFPAT
                     FALRSMIPGTAVGVLVGDLLFFWLALRLARQSGRNSITAMPLGLDTPSTLGMIFFVLG
                     PAFKAAQAAGMNEQAAATHTWHIGICAIFVSGIFKLLCSLGSNWIRRSFPRAGLLGSL
                     AAIALVLISFLPLLEILHSPIVGFVSLGVILTTLVARIELPFKIPGAVGGLLIGGAMY
                     YALQAAQSSGV"
     gene            940..1578
                     /locus_tag="VFB80_24615"
     CDS             940..1578
                     /locus_tag="VFB80_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN37046.1"
                     /translation="MKATLPEPMTEPQQLSSLTDEELLLGYRERGEERLFAELVRRYE
                     RELYSYLRRYLGDAEMAEDAFQAAFLQVHLKVQQFEAGRAVRPWLYTVATNQAIDAQR
                     RSRRHRMVSLDRTGPAGGEDVGKLLDLLTSKEPTPTEQLNADERRTWLHQAIEQLPES
                     FRDVIRLVYFQDLKYRETAEALQIPVGTVKSRMHAAVAKLNELWNQTQAASP"
     gene            1575..>2258
                     /locus_tag="VFB80_24620"
     CDS             1575..>2258
                     /locus_tag="VFB80_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37047.1"
                     /translation="MSSSSRDQLLGYLLGALEPEEHEQIADALARDPALRDEMRRLES
                     CVGRLGLADKTEYLEPPPDLVARTCQLVASQRRPVLVLRPTREAAAASRRYTWADLTV
                     AACALITAGAMLFPALAYSRGQAQIAGCQNNLRHVGFALQDFTERQPDNAFPTPELTG
                     NRSAAGVYTVHLNEGGFVRSPKVFLCPGAQNVRPTPGYHPPTFAELDRAIGAALAALQ
                     RSMGGDFGAN"
BASE COUNT          426 a          741 c          737 g          354 t
ORIGIN      
        1 aacgcccgaa ctctgggccg cttgcagtgc gtagtacatt gcgccgccga tcaaaagtcc
       61 gcccaccgcc ccgggaatct tgaagggcag ctcgatccgc gcgaccagcg tcgtcaggat
      121 cacccccagc gacacgaagc cgacgatcgg cgaatggagg atttccaaga gcggcagaaa
      181 gctgatcagc accagcgcga tcgccgccag cgagcccaat aacccggccc gcggaaacga
      241 ccgccgaatc caattggacc ccagcgagca gaggagcttg aagatcccgc tgacgaagat
      301 ggcgcaaatc ccgatgtgcc aggtgtgcgt ggcggctgcc tgctcgttca tgccggctgc
      361 ttgcgccgct ttgaaagctg gcccgagcac gaagaagatc atccccaggg tgcttggcgt
      421 gtccagccct aaaggcatgg ctgtaatgga gttgcggccg gactgacgcg ctagccggag
      481 ggcgagccag aagaacaaca ggtcccccac gagcacgccg acggcggtgc cggggatcat
      541 gctgcgcagg gcgaacgttg caggaaatgc aaagacgcca gagagcaaac tgaccgtgag
      601 caagaggttc gcgacgttat ctaacatcaa gccgaaaaag gcgttaacgt cgcccttagc
      661 ggcccaatcg tagcgcgtct gcttcaccgc gactcctctg gcatggcctt tgctttggag
      721 gagcggtgag tgtaggacgc ctctctgcga ctgtcaaatc ggccgaaaag ggccgatttg
      781 gcgcggccag gccggtgcga gcaagtgatg ctggcataag acttttcgtg gcctgaccgc
      841 taaaatgcaa ccacccgctg cgccggacgt gcgcgcgggc agtagccgca gagaactggc
      901 ccttgccggt gaaccatcct ttctgcggcc cgtaaagaaa tgaaagccac gttgccagaa
      961 ccgatgaccg agccccagca gctctcgtcg ctgaccgatg aggagttgct gctgggctac
     1021 cgcgagcggg gcgaagagcg cctgttcgcc gagttggttc gccggtacga gcgggagctg
     1081 tacagctatc tgcggcggta cctgggcgac gcggaaatgg cggaagacgc ctttcaggcg
     1141 gccttcctcc aggtccattt gaaggttcag cagttcgagg ccgggcgggc cgtccgcccc
     1201 tggctgtaca cggtcgccac caaccaggcg atcgatgcgc agcgccgcag ccggcggcac
     1261 cgcatggtca gcctggaccg caccggtccg gccggcggcg aggatgtcgg caagctgctc
     1321 gacctgctga cgagtaagga accgacgccg acggagcagc tgaacgccga cgagcggcgg
     1381 acctggctgc accaggcgat cgagcagttg ccggagtcgt ttcgcgacgt gatccgcctc
     1441 gtttacttcc aggacttgaa ataccgcgag actgccgagg ccctgcaaat ccccgtgggc
     1501 acggtgaaga gccgcatgca cgccgcggtg gccaaactca acgaattgtg gaaccagacg
     1561 caagccgcat ccccatgagc agttccagtc gcgaccagtt gctcggctat ttgctcggcg
     1621 ccttggagcc ggaagagcac gagcaaattg ccgatgcgct cgcccgcgat cctgccctgc
     1681 gcgacgaaat gcgccggctc gagtcctgcg tcggccgcct gggcctggcc gacaagaccg
     1741 aatatctcga gccgccgccg gacctcgtcg cgcggacgtg ccaattggtg gcgtcacagc
     1801 gccggccggt cctcgtcctg cggcccaccc gagaagccgc cgccgcctcg cggcgctata
     1861 cctgggccga tttgaccgtc gcggcctgtg ccctgatcac ggccggggcg atgctctttc
     1921 cggcgctggc ctacagccgc gggcaggctc agattgccgg ctgccagaac aaccttcgcc
     1981 acgtcgggtt tgcgctgcag gatttcaccg agcggcagcc cgacaacgct tttcccacgc
     2041 ccgagctgac cggcaaccgc agcgccgcgg gcgtgtatac ggtgcatttg aacgaggggg
     2101 gcttcgtccg ctcgccgaag gtcttcctct gccccggcgc gcagaacgtg cggccgacgc
     2161 ccggctatca tccgccgacg tttgccgagc tcgaccgcgc gatcggcgcg gcgctggccg
     2221 cgctgcagcg ctcgatgggg ggcgatttcg gcgcgaac
//
LOCUS       DASRJB010000931         6885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1663093, whole genome shotgun sequence.
ACCESSION   DASRJB010000931 DASRJB010000000
VERSION     DASRJB010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663093"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1613
                     /locus_tag="VFB80_24625"
     CDS             261..1613
                     /locus_tag="VFB80_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN37048.1"
                     /translation="MKVLFADDEPALQELMHLELPRMGHEVTVCPDGHTAVAALEKNT
                     YDCILVDLDMPGLNGIQVIAKCKELSPDTDAVVLTGKSSFETAVQALRHGAVDYLTKP
                     CKLVDLKALLLRIARQREWINKVRALQHQLERVEGKTELVGSSRGMEQVKRLIDKVAP
                     TNSTALILGETGTGKELVARAVHQQSLRAAKPFVAVNCGALPETLIESELFGHRKGSF
                     TGADDHRVGLFEVAHGGTIFLDEIGELPKSMQAKLLRVLESGEIRRVGDNNAFEVDVR
                     VVCATHRNLPEMVEAGGFREDLMYRINTFEIHLPSLRERAGDIGPLAEHLFRRFRPSL
                     RGTGPLFTPEAIDVLEKHTWPGNVRELANVIEHATILCDQPPLTPDHLPQRFASRRGP
                     LPKVRITGPITLRDLEMQAIYDALDRHGGNKPEAADELGISLKTLYNKLNQTALEKAA
                     "
     gene            2129..2341
                     /locus_tag="VFB80_24630"
     CDS             2129..2341
                     /locus_tag="VFB80_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37049.1"
                     /translation="MEIQKATDSRFQIETLEERIAPTANLNPGENNTNNTGNQPGDGG
                     GGGGGGNPGNNNPGQNNTNNPGNETP"
     gene            2437..2616
                     /locus_tag="VFB80_24635"
     CDS             2437..2616
                     /locus_tag="VFB80_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37050.1"
                     /translation="MESHKVEEPTRFQIDKLEDRIAPSSANFPPGQFPSGNPAFAPGR
                     SNPNEVPATNPSNDK"
     gene            2810..3025
                     /locus_tag="VFB80_24640"
     CDS             2810..3025
                     /locus_tag="VFB80_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37051.1"
                     /translation="MNPRSEASSKFQIDRLEERIAPAPSCPIPANDHALPPNNDGTLN
                     AAQHILAGPTEARIALVNWFGKAACNE"
     gene            3055..5550
                     /locus_tag="VFB80_24645"
     CDS             3055..5550
                     /locus_tag="VFB80_24645"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN37052.1"
                     /translation="MPAVQAPLPQRRTDLIIRPLGERGRFVVKDPGTGSYFHLGEQEH
                     FLLLQLCGEHAAAEVCRAFEERFAEPLSPGDLEGFLALARGKGLLQSTERGAGSPEQE
                     TAPAVSAASAPRPPVAPARLRQSILYWRTSLFDPDRFCTWLEPKIRWVWTRAFLAVSA
                     ACIVLALVVVATNGQQIVTNFARAFRWETLLLAWLALLLVTTLHELAHGLTCKHYGGE
                     VHEIGFLLMYFLPCFYCNVSDAWLFREKSKRLLVTFAGGYFELFLWALAVFVWRLTAL
                     DSLINYLAWVVLSIVGARIFFNLNPLIKLDGYYLLSDWLEIPNLRQRSWEYVAAHLRW
                     LLWGAARPARQERGRMLIGYGLASWLYSLAFLSLMLWGFFQFLWSSWGWAGLACVGWL
                     SWVSAGGLFRGISSGEVRNMFLLRHKRLFGWLLLLVAAPPTMQVITVEDRVTGPFQVR
                     SASRAELRAEVAGFLREVHFTEGDRVSPGAIVARLEVPDLASRIAQKQAEIREAEAKL
                     RLLEIGPRPEEVAEQKSRVARMEKWRDLAREDLARARQAYQEELSRLDRQIVQHTAEL
                     TAARDGFERAKELRRKGAIPQEQIEEALRKYDVAQAVLDQSHFQKRHRQALGTREAIA
                     GLDADSELARREKDLADSKSALTLLEIGARPEEKEAQRAALDRLREELRYWEAQQVRL
                     LVKSPVDGLVTTPRLKEKINHYLHEGDLICEVEEPSLLAVEVALTEQEAERVRVGQPI
                     ELHARALPLKSVRIEVERIAPLAVRGAAAAPPVPGDWETSGTVIIYGRLPNADTELRS
                     GMTGHARIYTGRRPLGTIALGRTLSWLRTEFWW"
     gene            complement(5678..6193)
                     /locus_tag="VFB80_24650"
     CDS             complement(5678..6193)
                     /locus_tag="VFB80_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37053.1"
                     /translation="MPDWTCQHCQADVDHGFDVCWQCGATATGERDADFVRERDVATL
                     PADHVRTIQCHVCGYRGKILFARYHFRAWAYVATPLLVLSVFGIVPLVIWLRSTRWMR
                     FKVCPRCATGKDLRDWDGEPTPEDESLWSKAQTRETTDYNRSWFRLLCVILATLLLPF
                     VISQIAWLLGH"
     gene            complement(6209..>6885)
                     /locus_tag="VFB80_24655"
     CDS             complement(6209..>6885)
                     /locus_tag="VFB80_24655"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZN37054.1"
                     /translation="FLNNPTAIRRVAAETSGVDDGVGQIMATLKRLNLDERTLVIYAA
                     DQGLMGGQNGLWGMGDHTRPAALFDQMLQVPLIYRQPGRIAGERTSDLVVSNYDFLPS
                     VLAYLGLQGKLPAGQNSPGRDYSAVLAGRDVQWQNTVFAEMENARAIRTPPWKYVARR
                     PDGPYELYDLATDPHERFNLFGQPKHTAVQQQLAQQLDEFFARYADPQYDLWKGGRSK
                     AGRLVR"
BASE COUNT         1256 a         2277 c         2190 g         1162 t
ORIGIN      
        1 cgaccacggc ggctcgatcg agccggccag cgacggtccg ggcaagggcg ccaccatgcg
       61 cgtcctcctc ccgcttgtag ctaaggacaa ggtccatgaa agaactgaaa aacgactcca
      121 agccgcttaa cctccgcgtg cacaacgccg acgcggagcc cgctgccaga agtactgcgc
      181 agcccgccgc cagaatcact gcgccggctg ccccgcttag ccgccgcgaa cacgcggcgt
      241 cggacgcccc ttccacgcgg ctgaaggtcc tgttcgccga cgacgagccg gccctccagg
      301 agctgatgca cctggagctg ccgcggatgg gccacgaggt caccgtctgc cccgacggcc
      361 acaccgcggt cgccgctctc gaaaagaaca cgtacgactg catcctcgtc gatctcgaca
      421 tgccgggcct gaacggcatt caggtcatcg ccaagtgcaa ggagctctcg cccgacaccg
      481 acgcggtcgt gctcaccggc aagtcgtcgt tcgagaccgc cgtgcaggct ctccgccacg
      541 gggccgtcga ttacctcacc aagccctgca agctggtcga tctcaaggcg ctgctcctgc
      601 gcatcgcccg ccagcgggaa tggatcaaca aggtccgagc cctccagcac cagctggagc
      661 gggtcgaagg caagaccgag ctcgtcggct ctagccgcgg catggaacag gtcaagcggt
      721 tgatcgacaa ggtcgcgccg acgaactcga ccgcgctgat cctgggcgaa accggcaccg
      781 gcaaggagct ggtcgcgcgg gccgtgcacc agcagagcct ccgggccgcc aagccgttcg
      841 tcgccgtgaa ctgcggcgcg ctgcccgaga cgctgatcga gagcgagctg tttggccacc
      901 gcaagggctc gttcaccggg gccgacgacc atcgcgtcgg cctgttcgaa gtcgcccatg
      961 gcggtacgat cttcctcgac gagatcggcg aactgcccaa gagcatgcaa gcgaagctgc
     1021 tccgcgtgct cgaaagcggc gaaatccgcc gcgtcggcga caacaacgcc ttcgaagtcg
     1081 atgtccgcgt ggtctgcgcg acgcaccgca acctccccga gatggtcgag gccggcggtt
     1141 ttcgcgagga cctgatgtac cgcatcaaca cgtttgagat tcacctgccg agcctccgcg
     1201 agcgggccgg cgacattggc cccttggccg agcacctctt ccgccgtttt cggccgagcc
     1261 tgaggggcac cggcccgctg ttcacgcccg aggcgatcga cgtgctcgaa aaacacacct
     1321 ggcccggcaa cgtccgcgaa ttggccaacg tcatcgagca cgccaccatc ctctgcgatc
     1381 agccgccgct gacgcccgac cacctgccgc agcgtttcgc cagccgccgc ggccccctgc
     1441 ccaaagtccg catcaccggc ccgatcaccc tgcgcgactt ggaaatgcaa gccatctacg
     1501 acgccctcga ccgccacggc ggcaacaaac ccgaagccgc cgacgagctc ggcatcagcc
     1561 tcaagacgct ctacaacaag ctcaaccaga ccgccctaga gaaggcggcc tgacgtgggg
     1621 cagacattcc tgtctgccat tccacattcg tcgttccagg ctcccacgcg tcactcgcca
     1681 ttggcgacgg gtcatgttgt ccgcgtaatg ccagaacggc gcgttgactc aagcgacaga
     1741 ttacgttttt cgccagaaat gccactgtac gggaccgcgc gctcgtggca tgtgaattgc
     1801 gacgatccgt aaaacgatgt gggggctcgc ccgtcgaacg accagcggcc gacgcgttgc
     1861 gcgtcggtgg ccgggcagag agacgaaggc gggcgactta tctttgatgc gcggagactc
     1921 taggtagttc ccgatcgggg tgcgcctgaa caaggctctg gcccggcagg caggtccgcg
     1981 ctacggacgg cggctgtgct gaatctggca ttcgcggcgc tcgtcctgcg cgctggccag
     2041 actcatgcgc gtgcattcgg cgctgaaccg gtttcccaac cggatagcgg acgttcgttt
     2101 tctctcctct ttttgacgag gtgtgcgcat ggaaatccaa aaggcaacgg acagtcgctt
     2161 tcagattgag acgctcgaag aacggattgc tcccaccgcc aacttgaatc cgggggagaa
     2221 caacaccaac aacaccggaa accagccggg cgacggcggc ggaggtggcg gaggtggcaa
     2281 tccgggcaac aacaacccgg gccagaacaa taccaacaat ccaggcaatg agacgccgta
     2341 gcgtcgtcat ccgatcgtcc cttgcgcagg ccgtgatcgg ccagcgtagt tttgttggcc
     2401 acaacttcta tttactcgcc cctgataagg tgtcccatgg aatcgcacaa agtagaagaa
     2461 ccgactcgct ttcagattga caagctcgaa gaccggattg ctccctccag cgctaatttt
     2521 ccacccggcc agttcccgtc cggcaatccc gcctttgctc ctggtcggag caacccgaac
     2581 gaggtgcctg cgaccaaccc gagcaacgac aaatgaaatc gagcgacgtt tctgaaattg
     2641 tgctcgacgt tggatcttag ccgtgttggc gagaattccg gcattcgggg gccgctgatc
     2701 gggcagaaat atgaacggcc cggtcggcgg cggatccccg ccgtgggaag cccccgatcg
     2761 gggttgtagt ttcgcttggt tcttcgtctc gattcacttg gaggctccca tgaatccgcg
     2821 ttccgaagca agcagcaagt ttcagatcga ccggctcgag gagcgaattg caccggctcc
     2881 ttcctgcccc attcccgcga acgaccatgc gctgccgccg aataacgacg gaacgctgaa
     2941 tgccgcccag catattttag ccggcccgac cgaggcgcgg atcgctttgg tcaattggtt
     3001 cggtaaggcg gcctgcaatg aatagccgct aaggccgccc gaggatccgc cgccatgccc
     3061 gcggttcagg caccgcttcc ccagcgccgg accgatctga tcattcggcc gctcggcgag
     3121 cggggacggt ttgtggtcaa agatccggga accggctctt actttcacct gggggagcag
     3181 gagcatttcc tgctcctgca actctgcgga gagcacgcgg cggccgaagt ctgccgggcg
     3241 tttgaagaac ggtttgccga gccgctctcg ccaggagacc tggaaggatt cctggcgctg
     3301 gcccgcggaa aagggctgct ccaaagcacc gaacgcggag cggggagccc ggagcaagaa
     3361 accgcccctg cggtctcggc agcgtctgct ccgcgacccc cagtggcgcc tgcccgcttg
     3421 cggcagagca tcctgtactg gcgaacgagc ctcttcgatc ccgatcgctt ctgcacctgg
     3481 ctggaaccaa aaatccgctg ggtttggacg cgcgcctttc tggccgtctc cgcggcgtgc
     3541 atcgtcctgg cgctcgtcgt tgtggcgacc aatggccagc agatcgtcac caacttcgca
     3601 cgggccttcc ggtgggagac gctcctgctc gcctggctcg cgctgctcct ggtgaccacg
     3661 ctccacgagc tggcccacgg cctgacctgc aaacactacg gcggcgaagt gcacgaaatc
     3721 ggctttctgc tgatgtactt cctgccctgc ttctactgca atgtctccga cgcctggctg
     3781 ttccgcgaaa agtcaaaacg gctgctggtg acgttcgccg gcggctattt cgagctgttc
     3841 ctctgggctc tggccgtctt cgtctggcgg ctgacggccc tcgacagcct gatcaactac
     3901 ctggcgtggg tcgtgctctc gattgtcggg gcacgaatct tcttcaatct gaatccgctg
     3961 atcaagctcg acggctatta cctgctgagc gactggctgg agattcccaa cctgaggcag
     4021 cgctcgtggg agtacgtagc cgcccacttg cgctggctct tgtggggcgc cgcccggccg
     4081 gcccgccagg agcgcggccg gatgctgatc ggctacgggc tggcgagctg gctctactcg
     4141 ctggctttcc tttcgctgat gttgtggggc ttctttcaat tcctgtggtc gagctggggc
     4201 tgggcgggcc tggcctgcgt cggatggctg agctgggtga gcgccggcgg tctgttccgg
     4261 ggaatttcca gcggggaggt gcgaaacatg tttctgctgc gccacaagcg attgttcggc
     4321 tggctgctgc tgctagtggc cgcgccgccc accatgcaag tcatcaccgt tgaggaccgc
     4381 gtcaccgggc cgtttcaggt ccgctcggcc agccgcgccg aactgcgggc ggaggtcgcc
     4441 ggctttttgc gcgaggtcca tttcaccgaa ggggaccgcg tctcgccggg ggccatcgtc
     4501 gcccggctgg aagtccccga cctggccagc cgcatcgctc agaagcaagc cgaaatccgg
     4561 gaagccgagg cgaaactgcg gctgctggag atcgggccgc ggccggaaga ggtggccgag
     4621 cagaaaagcc gcgtcgcgcg gatggaaaaa tggcgcgacc tggcgcggga ggacctggcc
     4681 cgggcccggc aggcctatca agaggagctg tcgcggctgg accggcaaat cgtccagcac
     4741 acggcggaac tgacggcggc cagggacggc ttcgagcggg ccaaggagct gcgccgcaaa
     4801 ggagcgatcc cgcaggagca gatcgaagag gccctgcgta agtacgacgt ggcccaggcg
     4861 gtcctggacc agtcgcactt ccagaagcgg catcgccagg cgctgggcac gcgcgaggcg
     4921 atcgccggcc tcgacgcgga ttcggaactg gcccgccgcg agaaggatct cgccgactcc
     4981 aaaagcgcgc tgacgctgct ggagatcggg gcgcggccgg aagagaagga ggcccagcgg
     5041 gccgccctcg atcgcctccg cgaggagctg cggtattggg aagcgcagca ggtgcgactg
     5101 ctcgtgaaga gccccgtgga cggcctggtc acgacgccgc ggctgaagga gaagatcaac
     5161 cactacctcc acgagggcga tctgatttgc gaagtcgaag agccgtcgct gctcgcagtc
     5221 gaagtggcct tgaccgagca ggaggcggag cgcgtccgtg tcggccagcc catcgagctg
     5281 catgcccggg cgctgccgct gaagtcggtc cgaatcgagg tcgagcggat tgccccgctc
     5341 gccgtgcgcg gcgctgccgc cgcgccgccc gttcccggcg actgggagac ctccggcaca
     5401 gtcatcatct atggccggct tcccaacgcc gacaccgagc tgcgctccgg aatgaccggc
     5461 cacgcccgca tctataccgg ccgccggccg ctcggcacga tcgccttggg ccgcacgcta
     5521 agctggctgc gcaccgagtt ctggtggtag cagtcgcaca aagccgcgac cttcgtcttc
     5581 cccgcctcgc cgtggttcgc tcgcgcgaac cagagccacc cgccaggaaa tcggccacaa
     5641 caaaggtggc cgagtcgggc gacctctgct taggctgtta atgccctagc aaccacgcga
     5701 tttgcgaaat aacaaacggc agcaggagcg tcgcgagaat cacgcagagc agccgaaacc
     5761 aggagcggtt gtagtcggtg gtctcgcggg tctgggcttt cgaccacagg gattcgtctt
     5821 ccggtgtggg ctcgccatcc caatcacgca ggtcctttcc agtggcgcag cgcggacaga
     5881 ccttgaagcg catccatcgg gtggagcgga gccaaataac tagaggaaca attccgaaga
     5941 cggagagcac gagcaaggga gtggccacat aggcccaagc ccgaaaatgg tagcgggcga
     6001 agaggatctt gccgcgatag ccgcagacgt ggcactggat cgtccgcaca tggtcggccg
     6061 gcagcgtcgc gacatcccgc tcgcgcacaa aatccgcgtc acgttcgcca gtcgccgtcg
     6121 caccgcactg ccaacagacg tcgaacccgt ggtctacgtc ggcctggcag tgctggcaag
     6181 tccagtctgg catgtggcct atcgtacttt atcgcacgag gcgcccggct ttggatcgcc
     6241 cgcccttcca caggtcgtat tggggatcgg cgtaccgcgc gaaaaactcg tcgagctgct
     6301 gcgccagttg ctgctgcacg gcggtgtgct tgggctggcc gaacaagttg aatcgctcgt
     6361 gcggatcggt cgcgaggtcg tagagctcgt aggggccgtc cggacggcgg gcgacgtatt
     6421 tccagggcgg ggtgcggatc gcgcgggcgt tttccatctc ggcaaagact gtgttctgcc
     6481 actggacatc gcggcccgcg agcactgcgg aatagtcgcg gcccggcgag ttctgcccgg
     6541 caggcagctt gccctgcagc ccgaggtacg ccagcacgct cggcaagaag tcgtagttgc
     6601 tgacaaccag gtcgctcgtt cgctcgcctg caatccgccc cggctgccga taaatgagcg
     6661 ggacctgcag catctggtcg aacaaggccg ccggccgagt gtgatcgccc atgccccaga
     6721 ggccattctg gccgcccatc aatccctgat cggccgcata aatcaccagc gtccgctcgt
     6781 cgaggttcag ccgcttgagc gtggccatga tctggcccac gccgtcatcg acgccgctgg
     6841 tctcggcggc cacgcggcgg atggcggtgg ggttattcag aaact
//
LOCUS       DASRJB010000932         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2248019, whole genome shotgun sequence.
ACCESSION   DASRJB010000932 DASRJB010000000
VERSION     DASRJB010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248019"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFB80_24660"
     CDS             complement(<1..284)
                     /locus_tag="VFB80_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZN37055.1"
                     /translation="MHRLPIGPLYYEFSRHNEPRLRIRPGESVVVESEDAFSGQVRTN
                     ADRRDRTQKPYGNPQTGPIYVEGAEPGDALAVKIHSIEPLIGQCATRTADL"
     gene            complement(286..1284)
                     /locus_tag="VFB80_24665"
     CDS             complement(286..1284)
                     /locus_tag="VFB80_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN37056.1"
                     /translation="MARPVTLFTGQWADLPLEQMARKTKQFGYDGIELACWGDHFEVD
                     KALADDGYCRRKRDLLNSLDLQCHAISAHLVGQAVCDIIDERHKSILPPAVWGDGKPA
                     GVNQRAAEELKNTARAAKRFGVGVVNGFTGSSIWHLLYSFPPVPPKMIDDGFKLFAQR
                     FNPILDVFAECGVKFALEVHPTEIAFDIYTAERALDAIGRRPEFGFNFDPSHLHWQGI
                     DSVEFLRAFPDRIYHMHVKDAIVTLNGKTGILASHLNFGDHRRGWDFRSPGRGTVNFE
                     EIVRELNRIGYNGPLSVEWEDSGMDREFGAKEAAGFVKQKDFAASGRAFDAAFEKS"
     gene            complement(1380..1910)
                     /locus_tag="VFB80_24670"
     CDS             complement(1380..1910)
                     /locus_tag="VFB80_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37057.1"
                     /translation="MGRCLAIALLLALPNIAQLQAAEPKLDEHLAPLAPLVGKTWRGE
                     FKNSTKEKPMFDVSRWEVTLKGKAVRCRHSVNDGVYGGESLIMWDPEKKTLVSFYFTT
                     AGFFTESTFEVKDGKFYSHEKVRGQNPGVAEVKAVTEIVDGKMHVKSQFLKEGTWVDA
                     HEITYVEAPDAKVVLD"
     gene            2108..>2921
                     /locus_tag="VFB80_24675"
     CDS             2108..>2921
                     /locus_tag="VFB80_24675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37058.1"
                     /translation="MQRVSRRRALGILAATAAAAGAPSAIGFGQEKAKAKGAKKAKAP
                     AAPEITFPPKLPGGVLSITETAEEFLQPPDTLKEGVAIAKTPPRVDFAYFPGQDYEGK
                     PWSNWGDATATLGKYYTAIGDHLAPAGNAIVFEYDAAGKSLRKLLDLKSLLKQPEGHY
                     SPAKFHSRVDLGSDGWLYCSTHRGSPRVTNDQYHYTGDWIVRCHPGTGAQEIVAHAPV
                     PKHCIPNGTLDPDRLIFYGGTAAGEGADGEGVQFFAYDCKSRKLIYSGPDGPA"
BASE COUNT          514 a          937 c          929 g          541 t
ORIGIN      
        1 agatcggccg tccgcgttgc gcactggccg atcagcggct cgatcgagtg aatcttcacc
       61 gccagcgcgt cgcccggctc tgccccctcg acatagatcg gccccgtctg cggattgccg
      121 tagggcttct gcgtgcggtc gcgccggtcc gcgttggtcc gcacctgccc gctgaacgca
      181 tcctcgctct ccacgaccac gctctccccc ggcctgatcc gcagccgcgg ctcgttgtgg
      241 cgggagaact cgtagtacag cggcccgatg ggaagtcggt gcatgttaag acttctcaaa
      301 cgcagcgtcg aaggcccggc cgctggcggc gaagtccttt tgcttgacga agccggcggc
      361 ttccttcgcg ccgaattcgc ggtccatgcc gctgtcttcc cattcgaccg agagcgggcc
      421 gttgtagccg atgcggttga gctcgcggac gatctcctcg aagttgaccg tgccgcggcc
      481 cgggctgcgg aagtcccagc cgcggcggtg atcgccgaag ttgaggtggc tggcgaggat
      541 gcccgtcttg ccgttgagcg tgacgatcgc gtccttcacg tgcatgtgat agatgcggtc
      601 ggggaaggcc cgcaggaact cgacggaatc gatgccctgc caatgcaggt ggctgggatc
      661 gaagttgaag ccgaactccg gccgccggcc gatggcgtcc aaggcccgct cggcggtgta
      721 gatgtcgaaa gcgatctcag tcgggtgcac ttccagagcg aacttcacgc cgcattcggc
      781 gaagacgtcg aggatcggat tgaaccgctg ggcgaacagc ttgaagccgt cgtcgatcat
      841 cttgggcggc accggcggga agctgtaaag caaatgccag atcgagctgc ccgtgaagcc
      901 gttgacgacg ccgacgccaa accgcttggc ggcccgggcg gtgttcttca gctcctcggc
      961 ggcccgctga ttaacgcccg ccggcttgcc gtcgccccag accgccggcg gcaggatgct
     1021 cttgtgccgc tcgtcgatga tgtcgcagac cgcctggccg accaggtggg cgctgatggc
     1081 atggcattgc agatcgaggc tgttcaaaag gtcccgcttg cggcggcagt agccgtcgtc
     1141 ggccagggcc ttatcgacct cgaagtggtc gccccagcag gccagctcga tgccgtcgta
     1201 gccgaactgc tttgttttgc gagccatctg ctccagcggc agatcggccc attgaccggt
     1261 aaaaagcgtg accggacgcg ccatttgcgt tgcctttcag aaggtgaagc ccgtgcgaaa
     1321 tgcagcgtca attgtggcgg ctgccgcgcg actctgcaac ggtcgcggtc gccgcgcgac
     1381 taatcgagca cgaccttcgc gtcgggcgcc tcgacgtagg tgatctcgtg ggcatccacc
     1441 cacgtgccct cctttagaaa ctgcgacttg acgtgcatct tgccgtcgac gatctcggtg
     1501 accgccttga cctcggcgac accgggattc tggccgcgga ccttttcgtg cgagtagaat
     1561 ttgccgtcct tgacttcgaa cgtgctctcg gtgaagaagc ccgcggtggt gaaataaaac
     1621 gagacgagcg tttttttctc cgggtcccac atgatcaggc tctcgccgcc atagacgcca
     1681 tcgttcaccg aatggcggca gcggacggct ttccctttca gggtcacctc ccaccgcgac
     1741 acgtcgaaca tcggcttctc tttggtcgag tttttgaact cgccccgcca ggtcttcccc
     1801 accagcggcg ccagcggcgc gaggtgctca tcgagtttcg gctccgctgc ctggagctgg
     1861 gcgatgttgg gcagagccag aagcagtgca atagcgaggc agcggcccat aaattggttt
     1921 cctgaagaat agatagatgg tgccgcgtca ggtgcggcgt tccgattatc ttctgtcagc
     1981 caatcgcgcg cctctaggcg gcagcggctg gacggaatga aatgaacggg cgaatttgac
     2041 gttttccact gcggcggcga agatagcaag tcccacccgt tttcgttccc accttggaga
     2101 gtctgtgatg caacgtgtct ctcgccgcag ggcacttgga attctcgcag ctacggccgc
     2161 cgcggccggc gccccctcgg ccatcggctt cggccaggaa aaggcaaagg cgaaaggggc
     2221 caaaaaagcc aaggcgcccg ctgcgccgga gatcacgttt ccgcccaagc tgcctggcgg
     2281 tgtactgagc atcaccgaga cggcggaaga gtttttgcag ccgccggata ccctgaaaga
     2341 gggcgtggcg atcgccaaga cgccgcccag ggtcgatttc gcctacttcc ccgggcagga
     2401 ctatgaaggc aagccctggt caaactgggg cgacgccacg gcgacccttg gcaagtacta
     2461 cacggcaatc ggcgaccacc tcgcgccggc cggcaacgcg atcgtgttcg aatacgacgc
     2521 cgccggcaaa tcgctgcgga aattgctcga cctgaagtcg ctcctcaagc agcccgaggg
     2581 gcactattcg cctgcgaagt ttcatagccg cgtcgatctg gggagcgacg gctggctcta
     2641 ctgctcgacg caccgcggtt cgccgcgggt aaccaacgat cagtaccact acacgggcga
     2701 ctggatcgtg cgctgccatc cgggaaccgg cgctcaagag attgtggccc acgccccggt
     2761 gcccaagcac tgcatcccca acggcacgct cgaccccgat cggctgatct tttacggcgg
     2821 gacagcggcc ggggaagggg ccgacggcga aggggtgcag ttcttcgcct acgattgcaa
     2881 gagccgcaag ctgatctatt ccggcccgga cggcccggcc c
//
LOCUS       DASRJB010000933         9267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3096006, whole genome shotgun sequence.
ACCESSION   DASRJB010000933 DASRJB010000000
VERSION     DASRJB010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096006"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFB80_24680"
     CDS             complement(<1..755)
                     /locus_tag="VFB80_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN37059.1"
                     /translation="MPHVLIIGGGFAGLNAAKGLGGMEGIEVTLVDRTNHHLFQPLLY
                     QVAMAGLSPAEIAAPIRSLLAGYRNIRVLQGEVRTLDLGGKKATVDFGELTFDYLILA
                     CGARHSYFGHDEWEEFAPGLKTLEQATEIRRRVLSAYEEAERNPLPEQRKRLLTFVIV
                     GGGPTGVELAGAIGEMSRFTLAKDFRNIDATLARVILLEAGPRILPMFSEQQAARATR
                     DLEHLGVQVWANSAVTKIDAEGVEIGSERIRSAT"
     gene            complement(951..1394)
                     /locus_tag="VFB80_24685"
     CDS             complement(951..1394)
                     /locus_tag="VFB80_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37060.1"
                     /translation="MLSALYTVLVLLAIGVVLWAIGRLLVNHWVGLRGRVRVPMGDVV
                     RMSGWGTADVNGIGFMESVRVVECERGWLVQLHWILGGGKLWLPRGQTHVTPPDENDR
                     SLADSALLESGPQRVRLEGDLAEFIAAPPPRTQEKRVRNFIRGME"
     gene            complement(1567..1710)
                     /locus_tag="VFB80_24690"
     CDS             complement(1567..1710)
                     /locus_tag="VFB80_24690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37061.1"
                     /translation="MNPRSPLLRPKDRGCGNQIRSFPARRMASEERSQCVTGHIVATA
                     VGG"
     gene            1736..2746
                     /locus_tag="VFB80_24695"
     CDS             1736..2746
                     /locus_tag="VFB80_24695"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZN37062.1"
                     /translation="MVRTRRRSGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRTQ
                     CLNHLKQIGIGLHNYHDTYRILPPAMTFPQGELAESSDLMGANWVISMLPYYEQQNLY
                     EAFNLRQPISSAANRTARGTRVAALLCPSDPNNRTAFRGNSTGEGDNWARGNYAANGP
                     NGRLDNRATWADPNLRGVMGWNLSVRFAEVTDGLSQTLMVGEIRAGLSADDRRGTWAL
                     GTAGSSALIWHGFTGDANGPNAPNADADDIEGCSTLVATIGAARLLKEKMSCWEPCPS
                     WQAAPRSLHPTGVCVVLCDGSVQFISNSVQTSGPFGSTMAAWDHFINSDGGVSVPAGV
                     IQ"
     gene            2756..3169
                     /locus_tag="VFB80_24700"
     CDS             2756..3169
                     /locus_tag="VFB80_24700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37063.1"
                     /translation="MPRFAFGCSLVCAALAAAGCGGGDNMYPVTGTVKFASGQVPQGE
                     VAVVRFEPADMAANAESKAASGKIAPDGSFRLTTIDPNDGALAGDYKVVLTVYKTYAG
                     RQSLINKKYTQAATTPLSAKVGPGGQSHFELVLDP"
     gene            3218..4408
                     /locus_tag="VFB80_24705"
     CDS             3218..4408
                     /locus_tag="VFB80_24705"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN37064.1"
                     /translation="MDNRLTTKTPDPPDPFSRRLLAALLVLTLGGCGQTAVLPKGPPL
                     ETAAAAADEFEAGLLVLSSPSLLAGIPGSGELTSEQLAAWLADEGNHRPLEVTLPRHL
                     RPSDAAATAIEFPPDNSLTRARVELGRQLFFDRRLSRGGRLCCADCHHPRRHFATYLA
                     KDGPLRDPLPAVNRLLAPAHFWDGRAASLEEQAAGPLENLHELDLPAAELTGRLAAIE
                     GYRRQFERIFGRLELHEVCQALACFQRAAVSLPAAWDLGQAGPAARRGAALFFGERGG
                     CSRCHAGPNFSDEHFHNVGTGAAAKSDEGRYAVTHREADRGAFKTPTLRNVAQTFPYG
                     HDARWLSLAEAVAWFAGVGAHSAAGNELTPLDLTAAEQQDLVAFLESLSSPPPPVETG
                     RLPQ"
     gene            4483..5715
                     /locus_tag="VFB80_24710"
     CDS             4483..5715
                     /locus_tag="VFB80_24710"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HZN37065.1"
                     /translation="MSDPYFQPLFAERIGGVNYGKGTEIYKFEKIKRAKRKALAEFPH
                     RPLVDFGIGENDAMAPESVRRRMAEEINRPENRGYMDNGNERFKQAVAAFMQRTMGVK
                     LDPATQINHSIGSKPALAMLPACFINPGDITLMTVPGYPVAGTHTRYYGGSVFRLPLL
                     AQNHFFPDFAVVPADVWERTKLLVLNYPNSPTGKTAPREFYAQVISLAKQHEFVVVQD
                     AAHALLSYDQPPQSFLSVPGAIDVGVELYSLSKGYDMIGWRIGWVCGQERIVRAFADV
                     KDNSDSGQFGAIQNAAAAALDDDEIPRRTRSKYERRLKKLVSTLSQAGFAAQMPGGTY
                     FLYVPAPKGLGGGTQFENAEAASQYLITQQSIVTVPWDDAGPFLRFSVTYEAADEAAE
                     DALMATAAERLAQCRLVF"
     gene            5797..6465
                     /locus_tag="VFB80_24715"
     CDS             5797..6465
                     /locus_tag="VFB80_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37066.1"
                     /translation="MLRFCAWSLITVVLCASLAAGQEYKVAKLESGPPAGALAPEIAA
                     LLAPGGYTILQGERAVCDIWPCKEWPIAEGAKTTGEVQYPLTPGQVFGAIRFPRKSAD
                     FRDQEIAAGVYVLRYAQQPVDGAHAGTFPTRDFCALLPAAKDRSAGVLDYKALIALGK
                     ETTGTAHPAIRPLLKPAAGDAPAIHADEEKMWTMVRFVGKTRQGPAVKDLPVELVVAG
                     VAAE"
     gene            6475..6966
                     /locus_tag="VFB80_24720"
     CDS             6475..6966
                     /locus_tag="VFB80_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37067.1"
                     /translation="MQNSECRIGRYVMALLAFSLWPSVALVAEPAAEFKLETLRGKVV
                     FLGEALQRRAGVTTVLEARERTLAIETKAGELIPLVEDTRGRAFRNDERLRKMEVELL
                     VRRYQVTPAVQIIRVFEIAADGSRFEIDYWCDVCSIVMYELKDCECCQGPIELRRTKV
                     AGR"
     gene            complement(7043..9241)
                     /locus_tag="VFB80_24725"
     CDS             complement(7043..9241)
                     /locus_tag="VFB80_24725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZN37068.1"
                     /translation="MMRSLVRTILLVAAGAMLVGSSSAAGPRVLGEGKQPNDVRLQPP
                     KDLDDYFPLVVPKSKEEWEARAARVRRQIMLSQGIWPLPERTPLNAVVHGKIDRGDYT
                     VEKVYFESVPGFLITGNLYRPKNASGKSPIVLFPHGHWNDGRFIDIGRQAVRKEIVIG
                     SERFEEGGRSLLQSLPVQCARMGCVCFHFDMIGYADSQQLSFQLGHRFGTQRPEMNSA
                     DNWGLYSPQAEAHLQSIMGLQTWGSVRALDFLLSLPDVDSSRVAVTGASGGGTQTMLL
                     SGIDPRVTASFPAVMVSTAMQGGCTCENACCLRVETGNIEFAGLFAPKPLGMTAANDW
                     TKEMATKGFPELQSLYKLFDAEKSVQLVNNIHFGHNYNYVNRSAFYAFLNKHFKLGLE
                     EPVVEEDYQRLSRDEMTVFDVAHPQPESGPEFERKLCRTLHEMAQQQLAALRPKDSAS
                     LAQWREVVGGAADVLVGRGLPAAADLDVQKTEETDLGDYLRVAGLLRNKSRGEELPVA
                     AWRPKKWDGRAAIWLSERGKAVLFAEDGSPAPEVKKLLDAGISVLGVDLLFQGEFLTN
                     GPSEVKNRTVQNNRQFAGYTYGYNHALLAQRAHDVLSAVAAMKNHPDKPKSVELIALD
                     GAAPIAAVALSACGNAVDRAVLDTRGFRFGKLTDYQGAGFLPGGAKYGDLPGFLSLAA
                     PTRIWLAGETAESAELARAAYKAAGSADAIALHSGTPDEAAAAAIAWLTK"
BASE COUNT         1533 a         3148 c         3070 g         1516 t
ORIGIN      
        1 gtggccgagc gaatccgttc gctgccgatt tcaacgcctt cggcatcgat cttcgtgacg
       61 gcgctattgg cccagacttg cacgcccaag tgctccaggt cgcgggtggc tcgcgcggcc
      121 tgttgctcgc tgaacatcgg gagaatgcgc gggccggctt ccagcaggat gacgcgggcc
      181 agcgtcgcat cgatattgcg aaagtcctta gccagcgtga aacggctcat ctcgccgatc
      241 gcgccggcca gctcgacgcc cgtcgggccg ccgccgacga tcacgaacgt gagcagccgc
      301 ttgcgctgct cgggcagcgg gttgcgttcg gcctcttcat aggccgacag cacgcggcgg
      361 cgaatctcgg tggcctgctc cagggtcttc aggcccggag caaactcctc ccattcgtcg
      421 tggccgaaat agctgtgccg cgcgccgcaa gccaggatca ggtagtcgaa cgtcagctcg
      481 ccgaaatcga cggtggcctt cttgccgccc aggtccagcg tgcggacctc gccctggagc
      541 acgcggatgt tgcgatagcc ggccagcagg ctgcggatcg gcgcggcgat ttccgccggg
      601 ctgagccccg ccatcgccac ttggtagagg agcggctgaa acaggtgatg gtttgtccga
      661 tcgacgagcg tcacctcgat gccttccatg ccgccgagcc ccttggcggc attcaggccg
      721 gcaaagccgc cgccgatgat caggacgtgg ggcatggagg gttcggggtt cagggttcag
      781 agttccggac agagcctgag agcataacgc tccggtgtgc gtgctcgcag cgcgtacctt
      841 tgctgatttc ggcccagcgg gattgcttca gggagccgct ctacgggatc aaccgcgctg
      901 ccctcttagc gtggcgcagc atcgcactgg ggcgattcga tgcggaacac ttattccatt
      961 ccgcggatga agttccgaac tctcttctct tgagtgcgcg ggggcggcgc ggcgatgaac
     1021 tcggcgaggt ctccttccag acggacccgc tgcggcccgg actcaagaag ggcactgtcg
     1081 gccagcgagc ggtcgttttc gtcgggaggc gtgacgtgcg tctggccgcg gggcagccag
     1141 agcttgccgc cgccgaggat ccagtgcaac tgcaccaacc agccgcgctc gcactcgacg
     1201 acgcggacac tctccatgaa gccgatgccg ttgacgtcgg cggtccccca gccggacatc
     1261 cgcaccacgt ctcccatggg cacgcgcacg cgcccgcgga ggccgaccca gtggttgacg
     1321 agcaggcggc cgatcgccca gagcacgacc ccgatcgcca gcagcaccag cactgtgtag
     1381 agcgcgctca gcatcgcttg tccccgcggc acgcggactt gaaccacggg cagaaagctt
     1441 gcaactgccg cgcccgccgc gcacagcgct gacacgccca agggagcctg tgccgtcccg
     1501 agaacatggg gagcgggcca gatcgggatc cccggcaaat aattaaaaat cgccggaaat
     1561 gatttgctac ccgccaactg ccgttgctac gatgtggccc gtgacgcact gcgaccgctc
     1621 ttcactcgcc atccggcggg ccgggaagct gcggatctgg ttgccgcagc cgcgatcctt
     1681 ggggcgcagc aacgggcttc gtggattcat ccttcactac ggggaagatc aggtcatggt
     1741 gcgcacgcgg aggcggagcg gctttacgct cgtggaattg ctggtggtca tcgccattat
     1801 cggcgtcctg gtggcgctgc tgttgccggc ggtgcaggcg gcccgcgagg ctgcccggcg
     1861 gacgcagtgc ctgaaccatc tcaagcaaat cggcattggc ctgcacaact atcacgacac
     1921 ctaccggatc ctgccgccgg ccatgacttt tccgcagggc gagctggccg agagcagcga
     1981 tctgatgggc gcgaattggg tcatctcgat gctgccgtat tacgagcagc aaaacctgta
     2041 cgaggccttc aacctgcggc agccgatttc gtcagccgcc aaccgcacgg cccgcggcac
     2101 gcgggtagct gccctgcttt gccccagcga tccgaacaac cggacggcgt ttcgcggcaa
     2161 cagcaccggc gaaggggaca actgggcccg cggcaactat gcggccaacg gccccaacgg
     2221 ccgcctcgac aaccgggcga cctgggccga cccgaacctg cgcggcgtga tgggctggaa
     2281 cttgtcggtc cgcttcgcgg aggtcaccga cgggctcagc cagacgctga tggtcggcga
     2341 aatccgcgcc ggactctcgg ccgacgaccg ccgcggcacc tgggccctgg gcaccgcggg
     2401 ctccagcgcc ctcatctggc acggcttcac gggcgacgcc aacggcccca acgcccccaa
     2461 cgccgacgcc gacgacatcg aagggtgcag caccctggtc gcgacgattg gcgcggcccg
     2521 attgctcaag gagaaaatgt cctgctggga gccctgcccg agctggcagg ctgctccgcg
     2581 gagcctgcac ccgacgggcg tctgcgtcgt gctgtgcgac ggcagcgtgc agttcatttc
     2641 caacagcgtg cagaccagcg gccccttcgg ctcgacaatg gccgcctggg accatttcat
     2701 caacagcgac ggcggcgtaa gcgtgcccgc tggcgtcatt cagtaggaag atggcatgcc
     2761 acggttcgct ttcggctgca gtctggtctg cgcggccctc gcagccgcgg gctgcggcgg
     2821 cggcgacaac atgtacccgg tgaccggcac ggtcaagttc gccagcggcc aggtgccgca
     2881 gggcgaagtg gccgtcgtcc gcttcgagcc ggccgacatg gccgctaatg cggaatcgaa
     2941 ggctgcctcc ggcaagatcg ctccggacgg gagcttccgc ctgacgacca tcgatcccaa
     3001 cgacggcgca ctggcgggcg actacaaagt cgtgctgacc gtctacaaga cgtacgccgg
     3061 ccgccaatcg ctgatcaaca agaagtacac ccaggccgcg accacgccgc tctccgccaa
     3121 ggtcggcccc ggcggccagt cgcacttcga gctggtgctc gatccatgag gttcaacgcg
     3181 agggggtcga gagtctttgt cgtcaacggc tttccaaatg gataatcgcc tgacgacaaa
     3241 gactcccgac cctcccgacc ctttcagccg ccggctgctc gcggcgctgc tcgtcttgac
     3301 gctcggcggc tgcggccaaa cggctgtctt gccgaagggc ccaccgctcg agacggctgc
     3361 ggcagccgcg gatgagtttg aggcggggct gctcgtgctt tcctcgccga gcctgctggc
     3421 gggcattccc ggtagcggcg aactgacgag cgaacaactc gccgcgtggc tggccgacga
     3481 gggaaatcat cggccgctgg aggtgacgtt gccgcggcac ttgcggccga gtgacgccgc
     3541 ggcaaccgcg atcgaattcc ctcctgacaa ttcgctcacg cgagctcgcg tcgaactggg
     3601 ccgccagctc ttcttcgacc gccgcttgtc gcgcggtggg agactctgtt gcgccgactg
     3661 ccaccatccg cggcggcatt tcgccacgta cctggccaag gacgggccgc tccgcgatcc
     3721 gctgccggcc gtgaaccgtc tgcttgcgcc agcgcacttc tgggacggcc gcgccgccag
     3781 cctcgaggag caggcagccg ggccgctgga aaacctccac gagctggacc tgccggcggc
     3841 ggagctgacc ggccggctgg cggcgatcga gggctaccgc cggcaattcg agcggatttt
     3901 cggccgcctg gagctgcacg aagtctgcca ggcgctggcc tgtttccagc gagcggcggt
     3961 gagcctgccg gcggcgtggg acttgggcca ggccggaccg gcggcccgcc gcggggcggc
     4021 gctgttcttc ggcgagcgcg gcggctgctc ccgctgtcac gccgggccga atttcagcga
     4081 cgaacatttc cacaacgtcg gcacgggcgc tgccgcgaag agcgacgagg ggcgatatgc
     4141 cgtcacgcac cgcgaagcgg accgcggcgc gttcaagacg ccgacgctcc gcaacgtcgc
     4201 gcagacgttt ccctacggcc acgatgcccg ctggctctcg ctcgccgaag ccgtggcctg
     4261 gtttgctggc gtcggcgcgc actccgccgc gggcaatgaa ctgacgccgc tcgatctgac
     4321 tgccgccgag cagcaggacc tcgtcgcctt cctggagtct ctctccagcc cgccgccgcc
     4381 cgtcgaaacc ggccgcctgc ctcagtagtc ggccgttgtc gctccgccag tgccctagta
     4441 acttgtactc tggtcccggc ccctggctcc tggctcatcc ccatgtccga cccctatttc
     4501 cagcccctgt ttgccgagcg aatcggcggc gtcaattacg gcaaggggac cgagatctac
     4561 aagttcgaga agatcaagcg ggccaagcgg aaggccctgg ccgagtttcc ccaccgcccg
     4621 ctggtcgatt tcggcatcgg cgagaacgac gccatggccc ccgagtcggt ccgccgccgc
     4681 atggccgagg agatcaaccg gccggaaaac cgcggctaca tggacaacgg caacgagcgc
     4741 ttcaagcagg ccgtcgccgc cttcatgcag cggacgatgg gtgtgaagct cgatccggcc
     4801 acgcagatca accattcgat cgggagtaag ccggccctgg cgatgctgcc ggcctgcttc
     4861 atcaatccgg gcgacatcac gctgatgacc gtgcccggct atccggtcgc cggcacgcac
     4921 acgcggtact acggcggcag cgtgttccgg ctgccgctct tggcccagaa ccatttcttc
     4981 ccggacttcg cggtggtgcc ggccgatgtg tgggagcgga cgaagctgct ggtgctcaat
     5041 tatcccaaca gccccaccgg caaaacggcg ccgcgcgagt tctacgccca ggtaatctcg
     5101 ctggcaaagc agcacgagtt cgtcgtcgtc caggacgccg cccacgcgct gctcagctac
     5161 gatcagccgc cgcagagctt tctcagcgtg cctggtgcga tcgacgtcgg cgtcgagctc
     5221 tattcgctga gcaagggcta cgacatgatc ggctggcgga tcggctgggt ttgcggccag
     5281 gagcggatcg tgcgggcgtt cgccgacgtg aaggacaaca gcgacagcgg acagttcggc
     5341 gcgattcaga acgcggccgc cgccgccctg gacgacgacg aaatcccccg tcgcacgcgc
     5401 tccaagtacg agcggcggct gaagaagctg gtcagcacgc tcagccaggc cggcttcgca
     5461 gcccagatgc ctggcggcac gtacttcctc tatgtccccg cgcccaaggg gctgggcggc
     5521 ggaacgcagt tcgagaatgc cgaggcggcg agccagtacc tgatcacgca gcagtcgatc
     5581 gtgacggttc cctgggacga cgccggaccg ttcttgcggt tcagcgtcac ctacgaagcg
     5641 gccgatgaag ccgcggaaga cgccctgatg gccaccgccg ccgagcgact ggcgcagtgc
     5701 cggttggtgt tctaggggcc gtttgtccgc aggatggcca cggcctatac tgaagttgcc
     5761 taccggttgg ttccctcctg ctgccgagac cttctcatgc tgcgtttttg cgcctggtcg
     5821 ttgattaccg ttgtcctctg cgcttccctg gcggcggggc aggaatacaa ggttgccaag
     5881 ctggaatccg ggccgccggc gggcgcacta gcgccggaaa tcgcggcgct gcttgcgccg
     5941 ggcggctaca ccatcctgca aggcgagcgg gctgtgtgcg acatctggcc ctgcaaggag
     6001 tggccgatcg cggagggcgc caagacgacg ggggaggtcc agtacccgct cacgccgggc
     6061 caggtttttg gggcgatccg ctttccgcga aagagcgccg acttccgcga ccaggagatc
     6121 gcggcgggag tctatgtgct gcggtatgcc cagcagccgg tggacggcgc gcacgcgggc
     6181 accttcccga cgcgcgactt ctgcgcgctg ttgccggcgg ccaaagaccg ttcggcgggc
     6241 gtgctggact acaaagcgct gatcgcgcta ggcaaggaaa cgaccggcac cgcccacccg
     6301 gccatccgcc cgctgctcaa gcctgcggcc ggtgacgcgc cggcgattca cgccgacgag
     6361 gagaagatgt ggacgatggt ccgcttcgtc ggcaagacga ggcagggccc ggcggtgaag
     6421 gacctgcccg tggagctggt cgtggcgggc gtcgcggcgg agtaggaatt cagaatgcag
     6481 aattcagagt gcagaatcgg ccgctacgtg atggccctgc tggcgttctc gctctggccg
     6541 agcgtggctc tcgtcgccga gcctgccgcg gagttcaagc tcgaaacgct ccgcggcaag
     6601 gtggtcttcc tgggcgaagc cctccagcgg cgggccggcg tgacgacggt gctggaagcc
     6661 cgcgagcgca cgctggccat cgagaccaag gccggtgagc tcatcccgct ggtggaagac
     6721 actcgcggcc gcgcattccg caacgacgag cggctgcgga aaatggaagt cgagctgctc
     6781 gtccgccgct accaggtcac gcccgccgtc cagatcatcc gcgtcttcga gatcgccgcg
     6841 gacggcagcc gcttcgagat cgactactgg tgcgacgtct gctcgatcgt gatgtacgag
     6901 ctgaaggact gcgaatgctg ccaggggccg atcgaattgc ggcggacgaa agtcgccggg
     6961 cggtagtcag aggtccgttg ccagcgatga caactgacct tacgccgaca tgcagcctgg
     7021 tcggccgaga tcaggccggc gactatttcg tgagccaggc aattgccgcc gcggcagcct
     7081 cgtcgggcgt gccgctatgc aacgcaatgg catcggcgga acccgcggct ttgtacgccg
     7141 cgcgggcgag ctcagccgat tcggccgtct cgccggcgag ccagatcctg gtgggcgccg
     7201 cgagcgagag gaagccgggc aggtcgccgt acttcgcgcc gccgggcagg aagccggcgc
     7261 cctgatagtc ggtcagcttg ccgaagcgga agccgcgcgt gtcgaggacc gcgcgatcga
     7321 ccgcgttgcc gcacgccgac agcgcgaccg ccgcgatcgg cgccgcgccg tcgagcgcga
     7381 ttagctcgac cgacttcggc ttgtcgggat ggttcttcat ggccgcgacg gcggagagca
     7441 catcgtgcgc tcgctgggcg agcagcgcgt ggttgtagcc gtaggtgtag ccggcgaact
     7501 ggcgattgtt ctgcaccgtc cggttcttga cttcagaggg accattcgtc aggaattcgc
     7561 cctggaatag cagatcgacg ccgaggaccg agatccccgc gtcgagcagc ttcttcacct
     7621 cgggcgcagg cgagccgtcc tccgcaaaca gcacggcctt gccgcgctcg ctgagccaga
     7681 tcgccgcgcg gccgtcccac ttcttcggcc gccaggccgc cacgggcagc tcttcaccgc
     7741 gggacttgtt gcgcagcagg cccgccaccc gcaggtagtc tcccaggtcg gtttcttccg
     7801 tcttctggac gtccaggtcg gccgccgccg gcaagccgcg gccaacgagc acgtccgccg
     7861 ccccgccgac gacttcgcgc cactgggcca gcgacgccga gtccttcggc cgcagcgccg
     7921 ccagctgctg ctgggccatc tcgtgcagcg tgcggcaaag cttccgctcg aactccggcc
     7981 cgctctccgg ctgcggatgg gccacgtcga acacggtcat ctcgtcgcgc gagagccgct
     8041 gatagtcctc ttcgacaacg ggctcttcca ggccgagctt gaagtgcttg ttgaggaacg
     8101 cgtaaaacgc gctgcggttg acgtagttgt agttgtgccc gaagtggatg ttgttgacga
     8161 gctgcacgct cttctccgca tcaaagagct tgtacagcga ctgcaactcc gggaagccct
     8221 tcgtcgccat ctccttcgtc cagtcgttgg cggcggtcat gcccagcggc ttgggtgcga
     8281 acaggccggc gaactcgatg ttgcccgtct cgacgcgcag gcagcaggcg ttttcgcagg
     8341 tgcagccgcc ctgcatcgcc gtcgagacca tcaccgccgg gaacgacgcg gtgacgcgcg
     8401 ggtcgatgcc tgaaagcagc atcgtctgcg tcccgccgcc gctggccccg gtcacggcga
     8461 cgcgcgagct gtcgacgtcc ggcagcgaga gcagaaagtc gagggcccgc accgagcccc
     8521 aggtctgcag gcccatgatc gactggaggt gggcttcggc ctgcgggcta tagaggcccc
     8581 agttgtcggc cgagttcatc tccggccgct gcgtgccgaa acgatggccg agctggaacg
     8641 agagctgctg gctgtcggcg tagccgatca tgtcgaaatg gaagcagacg cagcccatcc
     8701 gggcgcattg caccggcagc gactggagca gactgcggcc gccttcctcg aaccgctccg
     8761 agccgatcac gatttccttg cggaccgcct gccggccaat gtcgatgaat cgcccgtcgt
     8821 tccagtggcc gtgcgggaag agcacgatcg gcgacttgcc gctggcattc ttcggccggt
     8881 agaggttgcc cgtgatcaga aatcccggca cgctctcgaa atagaccttc tcgaccgtgt
     8941 agtcgccgcg gtcgatcttg ccatgtacga ctgcgttgag cggcgtccgc tccggcagcg
     9001 gccagatgcc ttgcgagagc atgatctgcc gccgcacccg cgcggcccgc gcttcccact
     9061 cttccttcga tttcggcacg accagcggaa agtagtcgtc caaatccttc ggcggctgca
     9121 agcgcacatc attcggctgc ttcccctccc ccagcacccg cggcccggcc gcggacgagg
     9181 aaccaaccag catcgcaccc gcagcaacta gaagaatcgt gcgaacaagc gagcgcatca
     9241 cggcaggact ccccggagag gttttct
//
LOCUS       DASRJB010000934         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3096639, whole genome shotgun sequence.
ACCESSION   DASRJB010000934 DASRJB010000000
VERSION     DASRJB010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096639"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFB80_24730"
     CDS             <1..1309
                     /locus_tag="VFB80_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZN37069.1"
                     /translation="MKKVIARGTAVATPQLGHSVHVEARGLAPDRWYWYRFRAGDATS
                     PIGRTRTLPAADVMPQRLKMAFASCQNFEQGLFTAYEQMAKDDLDLVFHLGDYIYETP
                     GRDGAVRKHNGPRSTKIKTLADYRQRHMQYRSDPLLRGMHALCPWLVTWDDHEFDNNY
                     AAGIQEAQRSGTPGDPLAFLEQRAAAYQAYYEMMPLRPRSLPQGPNMLLYRKASFGRL
                     AEFLVLDTRQYRSDQPNGDGLRPLNDEALQPANSLLGQQQRRWLEASLLASGGTWNVL
                     AQQVMMALVEIKTRDRSGYSMDQWPGAAHERMALLQFMADRKVPNPIVLTGDIHSNWV
                     NDLRVDDRRVDSPVVATEFVGTSISSGGNGSKEYSGLAQLQADNAGVRFHNRERGYVR
                     CTITPESWQSDYVVVEDVTKPGGNVLTRASFVVEAGTPGAKPT"
     gene            1419..>2390
                     /locus_tag="VFB80_24735"
     CDS             1419..>2390
                     /locus_tag="VFB80_24735"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN37070.1"
                     /translation="MLTADRLDSILRTLPRMTIGLVGDLFLDRYLELAPGSRELSIET
                     GLEAYQVERVRNSPGALGTVLNNLAALGVGLLVPVTAIGDDGHGYDLLKELRKLPVDA
                     AHVLCQSERLTPTYTKPLRFVDGAWQELNRLDVRTRWPLSAAAMAEICRRVEVVFRSS
                     DGLVVLDQLVDEDCGVVNSAVRDTLARLADESPQKLIFVDSRRFLAKFTAGVLKGNRS
                     EIGTVGNALRGAAGADDAAVNESLAAITRRTGRLAFCTAGDRGIVVAEPGQDIVLVPG
                     FPVAGPIDIVGAGDAATSGIVCALLAGASPREAAALGNLVASITIQQL"
BASE COUNT          405 a          786 c          804 g          395 t
ORIGIN      
        1 gatgaaaaag gtgatcgccc gcggcacggc ggtggcgacg ccgcagctgg ggcactcggt
       61 ccatgtggag gcccgcggcc ttgcgccgga ccgctggtac tggtatcgct ttcgcgccgg
      121 cgacgcgacc agcccgatcg ggcggacgcg gacgctgccg gcggccgacg tcatgccgca
      181 gcgcctgaag atggcgtttg cttcctgcca gaattttgag cagggtctgt tcaccgccta
      241 cgagcagatg gcgaaggacg acctggacct ggtgttccat ctgggggact acatctacga
      301 gacgccgggc cgcgacggcg cggtccgcaa gcacaacggc ccgcggagca cgaagatcaa
      361 gacgctggcc gactatcgcc agcggcacat gcagtaccgc tccgatccgc tgctccgcgg
      421 catgcacgcg ctgtgcccgt ggcttgtcac ctgggacgac cacgagttcg acaacaacta
      481 tgccgccgga attcaggagg cgcagcgcag cgggacgccg ggcgatccgc tggcgtttct
      541 ggaacagcgg gccgcggctt accaggcgta ctacgagatg atgccgctcc gcccgcgttc
      601 gctgccgcaa gggccgaaca tgctgctgta ccgcaaggcc tcgtttggac ggctggcgga
      661 gtttcttgtg ctcgatacgc ggcagtaccg cagcgaccag cccaacggcg acgggttgcg
      721 gccgctcaac gacgaggcgc tgcagccggc gaactcgctc ctgggccagc agcagcgccg
      781 ctggctcgaa gcgagcctgc tcgcctctgg cggcacgtgg aacgtgctgg cgcagcaggt
      841 gatgatggcc ctggtcgaaa tcaagactcg cgaccgctcg ggctattcga tggaccagtg
      901 gcccggtgcg gcccacgagc ggatggccct gctgcaattc atggccgacc gcaaggtgcc
      961 caaccccatc gtgctgacgg gcgacatcca ttccaactgg gtcaacgacc tgcgggtcga
     1021 tgaccgccgg gtcgattcgc ccgtggtagc caccgaattc gtcggcacca gcatctccag
     1081 cggcggcaac ggctccaagg aatactccgg gctggcccag ttgcaggccg acaacgctgg
     1141 agtgagattt cacaatcgcg agcggggcta cgtccgctgc acgatcacgc cggaaagctg
     1201 gcagagcgat tacgtcgttg tcgaggatgt gacgaagccc ggcgggaacg tgctcacgcg
     1261 ggcctcgttc gtcgtcgagg ccgggacgcc gggagcgaag ccgacttgag gtcctagcaa
     1321 tttgggattg ggaatttggg atttgggatt tgggattggg ggaacaaatc ccaaatcccc
     1381 aatcctcaat cccaaatcga attaaccaac accagcccgt gctcaccgcc gaccgactcg
     1441 attcgattct ccgcacgctg ccgcgaatga cgatcggcct cgtgggggac ctgttcctcg
     1501 accgatacct ggagctggcg cccggcagcc gcgaattgtc gattgagacg ggcctggagg
     1561 cgtatcaggt cgagcgggtg cggaattcgc cgggtgcgct gggcaccgtg ctcaataacc
     1621 tggcggcgct gggcgtcggt ttgctcgtgc cggtgacggc gatcggcgac gacggccacg
     1681 gctacgacct gctcaaggaa ctgcggaagc tgccggtcga tgcggcgcac gtgctctgcc
     1741 agagcgagcg gctgacgccg acctatacga agccgctacg tttcgtcgac ggcgcatggc
     1801 aggagctgaa ccggctcgac gtgcggaccc gctggcccct ttcggccgcg gcgatggccg
     1861 agatttgccg ccgcgtggag gtcgtctttc gcagctccga cggcctggtc gtcctcgacc
     1921 agctcgtcga tgaagactgc ggcgtggtga actcggccgt ccgcgacacg ctcgcccgcc
     1981 tcgccgacga atcgccgcag aagctgatct tcgtcgatag ccggcggttt ctggcaaagt
     2041 tcacggcggg cgtgctgaag ggcaaccgct cggagatcgg cactgtcggg aatgcgctcc
     2101 gtggcgctgc gggcgcggac gacgctgcag tgaatgaatc gctcgccgcg attacccgtc
     2161 gcacgggccg actcgccttt tgcacggccg gcgatcgcgg catcgtcgtc gccgagcccg
     2221 gccaggacat tgtgctcgtc cccggcttcc cagttgctgg accgatcgac atcgtcggcg
     2281 ccggcgacgc tgccaccagc ggcatcgtct gtgctctcct ggccggcgca tcgccgcgcg
     2341 aagccgccgc cctgggcaac ctcgtcgcct cgatcacgat ccaacaactc
//
LOCUS       DASRJB010000935         9051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2249504, whole genome shotgun sequence.
ACCESSION   DASRJB010000935 DASRJB010000000
VERSION     DASRJB010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249504"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFB80_24740"
     CDS             complement(<1..252)
                     /locus_tag="VFB80_24740"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN37071.1"
                     /translation="MSKSPIGLIGVGLLGAALAERMIRGGWEILGFDVRPGQLDALAA
                     TGGKPAGSAVEVGAACDIVALCLPDSHVVAAVAAQIDETL"
     gene            342..1121
                     /locus_tag="VFB80_24745"
     CDS             342..1121
                     /locus_tag="VFB80_24745"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN37072.1"
                     /translation="MLQHYLLVSLIILASSVVQGAVGFASGLFGTPLLMLTGMSLPDA
                     VAISLVTSAFQNCIAAWQLRRDIDFARALRPMLIRFAMLPVGAAALYAVGKENNALAG
                     QIVGAIILGIVAVQQLVQVPPQPQLHAAWEWLAFGLGGFLLGLCGMGGPPMMLWALAH
                     DWPMNRLRAFMYFIFVTGLIPQGLLLWLTFGNEILWPMLLGLGVLPAVLVGLYLGLYL
                     GRILPDRFLRSVSVAVLVLIALSAIASPYVLSSLSWLRGYQ"
     gene            complement(1112..2053)
                     /locus_tag="VFB80_24750"
     CDS             complement(1112..2053)
                     /locus_tag="VFB80_24750"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN37073.1"
                     /translation="MRVALRQLAVRCGLALLCGAAAGLVRAAVPENPLRPAPARSDSP
                     AIQPEPLAHSLVSAEAQVSEPASVVPEMLPAPAGDYDAAAPLHYPIDEADVFSGLVPA
                     WLAEPWFAHSDPNDPHRHIGLGQPLIGTSWRNRPWYVGTFIGGVLLGELQSDVHQNDT
                     SFLGLRLGIDFDHYWGFEGRWAFARPQLFDGADVPLDGPSRDYFTDFSLAWYPLGDAR
                     WRPYVVAGVGFQTFRFHNDLDQRISETIFSVPIGFGVKYFYGPWCSLRFDFVDNIAFG
                     NERVSAANNIGLMAGVEYRFGGRRQSYFPWHNNTSYW"
     gene            2311..2775
                     /gene="rimI"
                     /locus_tag="VFB80_24755"
     CDS             2311..2775
                     /gene="rimI"
                     /locus_tag="VFB80_24755"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZN37074.1"
                     /translation="MIRRDMAEVLAIEGQCFEFPWSEEDFIRCLRQRNCIGMVAEHNE
                     RVVGFMIYELHKSRLHILNFAVHEHHRRRGIGSQMVKKLSGKLSPQRRSRIMLEIRET
                     NLPAQLFFRALGFRAISVLRGFYDDTPEDAYLMQLRYSAAAAAEQRAARLAG"
     gene            2964..7739
                     /locus_tag="VFB80_24760"
     CDS             2964..7739
                     /locus_tag="VFB80_24760"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HZN37075.1"
                     /translation="MRWIWNRFYSRAKPRRARARAVRPLVEPLESRSLLAVVTALESG
                     LWSDAATWDGNAPPTAVDTVVIPSGANVTLNANPTVAGMTIQAGGQLTFDPNATRVLS
                     SSGNIEVAGMLQMRPASAAIRHELSFIGIVETNFVGGGEEVLASDVGLWIVGPGQLDA
                     VGADKTPWSHLSAAVSAGATTFTVDDATGWRVGDLISITPTSPPSHSGSNLSWTAYDD
                     RNITAISGNTITISATNPLTYDHPVVNDTWYAEVLNLSRNVKIHGTSSSSGRSHIMFT
                     EITQPQELRNVEIYHMGPRKHRNGDSQDDLIPGRYPLHFHHAGDGTRGSLIENVVVRN
                     SGNHSFVTHASHGMTFRGTIAHNVFEDAYWWDPDTDTSEGNGTNDTLYDHAVASKVFH
                     RGMYLGTPTVDTFRTSGFRLGQGQRNSVINSVAVGMQGDLQGSGFQWPERTGEFVWTF
                     QGNVAHNGATDGIFTWQNESSFLHVIEDFTAYYNGKHGIEHGAYGNEYLYRDSTLYGN
                     LNSGFLIHSVSNSWGKPLTLDNLTIDGAEISWFGINTQKHRVSSHGKPTIIQNSTIKN
                     LRTAPAEDSRSGTAIAWTGTGGEIASIPPEQYIIRNITFDGPYADLFHLSTTWTDFDA
                     DGVIDFGDINADGVIDSEDIGPTQTGGPELKGINPTSNIYVELQSGEAFRLRSSLYQW
                     PNHGNLPLAPGWSGRKEAIRAAGTPIVDAGVNQSIFAPNNAFTLDATVSNPAPGGALT
                     TTWSNVSGPGTVSFGDASAVDTMATFSTLGTYVLRLTASDGTFTHFDDVTVYYNPANP
                     ATSFTENWSGASGAPWPSTWTRQGPSNNWIVDINNNQGHIKRNSVNVSSGVFYLNNAS
                     AQDADISVKIKSNGSGIVTGVVARRSDSDPDTYFLLAFDSGDLNIYAVVDGAKYLLDR
                     DDYINTGSGFNWMRFLVESNPDGSVRLRGRYWADGNTEPSGWHVEVPALSQFPQLAGK
                     SGRSGLHIDTQGGSFREVWYDSFTANTNLAPVVTASVEPFPVGSAVGTANLDGTIADD
                     GVAGPLTATWTKISGPGSVTFANKNAVDTTAAFSLKGTYVVRLTANDGNKSAYADAIV
                     NVVQESFTGADGSAWPSQWVQQNGAGAATADIQAGQGRVTNGTTAINNVSLFNSTTQA
                     ENVDLSVRFRVDSNLVIPGFRARRADMRANTFYSAYTLPGTSGNVGIQAVVRGVATTL
                     ATGDFPLTANADYYMRLQVETNAVGTTDLRVKLWDAADAEPGAWTMQLTSGTPELQRV
                     AGRFGISTGFYYAPSKTAYYDDFKAIAQVKENAAPTVAAIAHLDPAAAVWTVNLNGTV
                     IDDRRPANSLNTTWTKVSGPGTVAFGNAAAVDTTATFSSSGAYVLRLTANDGARSKAK
                     DVAINVVEETFSAADGSPWSSQWTRQQIGGTAPAATVQGNEGQIANAGGGFYGNVLMV
                     NNAAASENVDQSVKFRLNGDFAWTSLIARRDDANPDTWYAAEINVKPGGTGALTIQKV
                     VDGAATVLGTANFAVAPDTNYLMRFQVTSTRTGKTDLRIKVWAEGSSEPSAWTLAVLG
                     DATAQLQGVAGRVGIRTLYYLAPPSRTAWYDDYRALLLG"
     gene            7765..8829
                     /locus_tag="VFB80_24765"
     CDS             7765..8829
                     /locus_tag="VFB80_24765"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HZN37076.1"
                     /translation="MRMLVAILLVLGCRTVQADTFAYVSLAKDKKIAVYNVDPSTGAL
                     AHVADAPCGGEPAALVANPTRTSLFASLRPEGKLVAFRIDPRTGTLAHLNTIDAGIDP
                     AHLSTDKQGRFLLAAYYVAAKVTVHAIEADGSLRPQPVESVPTAEKAHAILLDPANRF
                     AFVPHTGPNAIFQFAFDAKTGGLKPGSPARLATPPNTGPRHIVFHPRLDMAYVSNEQG
                     GSVTAWRYDKSAGSLAAVQTISTLPASYGQRNACAEIKLHPTGTFLYVANRGHDSIAR
                     FRVDGRSGELTALGQTSTEKTPRSFDLDPAGKFLYVCGEATDRIAGYRVSPDSGDLAP
                     VVSGPVGKMPWWILMVDVAR"
BASE COUNT         1667 a         3097 c         2825 g         1462 t
ORIGIN      
        1 gagcgtttca tcgatctggg ccgcgacggc cgctacgaca tgcgagtcgg gcaagcaaag
       61 cgccacgatg tcgcaggccg ctccgacctc gactgcgctg ccggccggct tgccgcctgt
      121 cgccgccagc gcatcgagct ggcctggccg gacgtcaaag ccaaggattt cccagccgcc
      181 gcggatcatc cgctcggcca gcgcagcgcc gagcaaaccg acgccgatca atccgattgg
      241 tgatttgctc atcggccgct aaggtagagg cgcgagtggt gagtggtggg tcgtggtctt
      301 tcaccactca ccaccgacca ctcaccactc actgccaggc aatgctccag cactacctgc
      361 tcgtcagcct gatcatcctg gccagcagcg tcgtgcaggg agcggtaggc tttgcctcgg
      421 gactgttcgg caccccgctg ctgatgctca caggcatgtc gctgcccgat gcagtagcga
      481 tcagcctggt gacatcggcg ttccagaatt gtatcgccgc ctggcaactg cggcgcgaca
      541 tcgacttcgc ccgggccctg cggccgatgc tgattcgctt tgccatgttg ccggtgggcg
      601 ccgccgcgct ctacgccgtc gggaaggaaa acaatgccct ggccggccag attgtcggtg
      661 ccatcatcct cggaatcgtc gcggtgcagc agctggtgca ggtgccgccg cagccgcagc
      721 tgcatgccgc ttgggagtgg ctggcgttcg gcttgggcgg ctttttgctc ggcctgtgcg
      781 ggatgggcgg gccaccgatg atgctctggg ccctggctca cgactggccc atgaaccgcc
      841 tgcgggcctt catgtacttc attttcgtca ccggattgat cccgcaaggg ctgctgctct
      901 ggctgacgtt cggcaacgag attctctggc caatgctgct gggcctgggc gtcctgccgg
      961 cggtcctcgt cgggctgtat ctcggcctct atctcggccg catcttgccc gaccgcttcc
     1021 tgcgcagcgt gagcgtcgcg gtgctggtgc tgatcgcgct cagcgcgatc gcgtcgccgt
     1081 atgtgctcag tagcttgagt tggctccgcg gctaccaata actggtgttg ttgtgccacg
     1141 gaaaatagct ctgccgccgg ccgccgaagc ggtactcgac gccggccatc aggccgatgt
     1201 tgttcgcggc gctcacccgc tcgttgccga aggcgatgtt gtccacgaag tcgaaccgca
     1261 ggctgcacca cggcccatag aagtacttta cgccgaagcc gatcggcacg gaaaagatcg
     1321 tctcgctgat ccgctggtcc aagtcgttgt ggaagcggaa cgtctgaaag ccgacgccag
     1381 cgacgacgta cggccgccag cgggcgtcgc ccagcggata ccaggccagc gaaaaatccg
     1441 tgaagtaatc gcggctgggt ccgtcgagcg ggacgtccgc cccgtcgaac agctgcggcc
     1501 gggcgaaggc ccagcggccc tcgaagcccc agtagtggtc gaagtcgatg cccagccgca
     1561 agccgagaaa cgacgtgtcg ttttgatgaa cgtcggactg cagctcgccc agcaggacgc
     1621 cgccgatgaa ggtgcccacg taccagggtc ggttccgcca gctggtgccg atcagcggct
     1681 gccccaggcc gatgtgccgg tgcggatcgt tcggatcgct gtgcgcgaac cagggctcgg
     1741 ccagccacgc cggcaccagg ccggaaaaga cgtccgcttc gtcgatcgga tagtgcagcg
     1801 gcgcggcagc gtcgtaatcg ccggccggcg caggcagcat ctcgggcacg accgacgccg
     1861 gctcgctgac ttgggcttcg gccgagacga gggaatgagc cagcggctcc ggctgaatag
     1921 cgggactgtc gctgcgggcc ggcgccggac gcagcggatt ctccggcacg gccgcgcgca
     1981 ccaggcccgc ggctgccccg catagcagag ccagcccaca ccgaacggca agttggcgca
     2041 gagccacgcg catgatcccg cttccgcctg gaaaaacagg cggcggatta tcgatgcgac
     2101 tcgcggcggc gtcaattgca agaatgcgag ccagtcgccc gctagcagtg gcccatgccg
     2161 attcgtcttg gctgcacctg cgacctggat tattcttcgc cccgctgccg ccgacggtag
     2221 ccgctggccc tcagggagga gaacgccatg accaccacgc aagtcgccat ccccgccaag
     2281 aacgcgccga tgccggtgca cattcgctgg atgatccgcc gggacatggc ggaagtgctg
     2341 gcgatcgagg ggcagtgttt tgagtttccc tggtcggagg aggacttcat ccgctgcctc
     2401 cgccagcgaa attgcatcgg catggtcgcc gagcacaacg agcgcgtggt cggcttcatg
     2461 atctacgaat tgcacaagag ccgcctgcac atcctgaact tcgccgtcca cgagcaccat
     2521 cgccgccgcg gcatcggcag ccagatggtg aaaaagctca gcggcaaact ctcgccgcag
     2581 cgccgcagcc ggatcatgct ggagattcgc gagacgaacc tgccggccca gctgttcttc
     2641 cgggcgctgg gcttccgggc gatttcggtc ctgcggggct tttacgacga cacgccggag
     2701 gacgcctacc tgatgcagct gcgctattcg gcagccgccg cggccgagca gcgcgccgcc
     2761 cggctggcgg gctagaccgc gtagccgacg cagcttgcgt cggagttatt cgcaccagtg
     2821 cacgcctcgc ccgccaggcg cggcgaggcg acagcttgaa ttggcgcggc gcggcaaact
     2881 agaatccgcg atcatctggt cggggccttt ctcatcccgg ctcgaacttg ggccagccct
     2941 gtatcgctcc caggaacgcc gccatgcgct ggatctggaa ccggttctac tcaagggcga
     3001 agccgcgccg cgctagggcc cgagccgttc gcccgctcgt cgaaccgctg gagtcgcgga
     3061 gccttctggc cgtagttacc gcgctcgagt ccggcctgtg gagcgacgcg gccacctggg
     3121 atgggaatgc tccgccgact gccgtcgata ccgttgtcat cccgtcgggc gcgaacgtca
     3181 cgctcaacgc taacccgacg gtagctggaa tgaccatcca ggccggcggc cagctcactt
     3241 tcgatcccaa tgccacgcgg gtgctgagca gctcgggcaa catcgaagtg gccggcatgc
     3301 tgcaaatgcg ccccgcttcg gccgcgatcc ggcacgagtt gtcgttcatc ggcatcgtcg
     3361 aaacgaactt cgtgggcggc ggcgaagagg tcctcgcatc cgatgtcggc ttgtggatcg
     3421 tcgggccggg ccagctcgat gccgtcggcg ccgacaagac tccctggagt cacttgagcg
     3481 ccgccgtcag cgccggggcg accacgttca ccgtcgatga cgcaaccggc tggcgcgtgg
     3541 gggacctgat ttccatcact cccacgtcgc cgccgtcgca cagcggcagc aacctctcct
     3601 ggaccgccta cgacgaccgg aacatcacag cgatcagtgg caacaccatc acgatcagcg
     3661 ccacgaaccc cctgacctac gaccacccgg tcgtgaacga cacctggtat gccgaggtct
     3721 tgaacctgtc gcgaaacgtg aagattcacg gcaccagcag ctcgtccggc cgctcgcaca
     3781 tcatgttcac ggagatcact cagccgcagg agctgcgcaa cgtcgagatc tatcacatgg
     3841 gtccgcggaa gcaccgcaac ggcgacagtc aggacgacct catccccggc cgctacccgc
     3901 tgcacttcca ccacgccggc gacggcaccc gcggctcgct gatcgagaat gtcgtggtcc
     3961 gcaactcggg caaccactcc ttcgtgaccc acgccagcca cggcatgacc ttccgcggca
     4021 caatcgccca caacgtattc gaggatgcgt actggtggga tccggacacc gacaccagcg
     4081 aaggcaacgg caccaacgac acgctctacg accacgccgt cgcctcgaaa gttttccacc
     4141 gcggcatgta cctgggaact cccacggtcg ataccttccg cacctccggc ttccgcctgg
     4201 ggcaaggcca gcgcaacagt gtgatcaaca gcgtcgctgt cggcatgcag ggcgacctgc
     4261 agggcagcgg cttccagtgg cccgagcgga cgggcgagtt cgtgtggacc ttccagggca
     4321 acgtcgccca taacggcgcg accgacggca tcttcacctg gcagaacgag agctcgttcc
     4381 tgcacgtcat cgaagacttc accgcctact acaacggtaa acacggcatc gaacacggcg
     4441 cgtacggcaa cgaatacctc taccgcgact cgacgctcta cggcaacttg aactccgggt
     4501 tcctaattca ctcggtcagc aacagctggg gcaagcccct cacgctggac aatctcacca
     4561 tcgatggcgc ggagatcagc tggttcggca tcaacaccca gaagcaccgc gtcagctccc
     4621 acggcaaacc gaccatcatc cagaacagca cgatcaagaa tctccgcacg gcccctgccg
     4681 aggactcgcg cagcggcacg gccatcgcct ggaccggcac cggcggcgaa atcgccagca
     4741 tcccgcccga gcagtacatc atccgcaata tcaccttcga cggcccctat gcggacctgt
     4801 ttcacctcag cacgacctgg accgatttcg acgccgacgg cgtcatcgac tttggcgaca
     4861 tcaacgccga cggcgtgatc gacagcgagg acatcggccc cacgcaaacc ggcgggccgg
     4921 agctgaaggg gatcaacccg acctccaaca tctacgtcga gctgcaaagc ggcgaagcat
     4981 tccgcctgcg ctcctcgctc taccaatggc cgaatcacgg caacctgccg ctagcgccgg
     5041 gctggagcgg ccgcaaggag gcgatccgcg ccgccggcac gccaatcgtc gatgcgggcg
     5101 tgaaccagtc gatcttcgcg ccgaacaacg ccttcacgct ggacgcgacg gtgagcaacc
     5161 ccgcgcccgg cggcgcgctg accaccacct ggagcaacgt gagcggtccc ggcacggtgt
     5221 cgttcggcga cgccagcgcc gtggacacta tggccacatt ctcgacgctg ggaacgtacg
     5281 tgctccgcct caccgccagc gacggcacgt tcacccattt cgacgacgtg acggtctact
     5341 acaacccggc caaccccgcg acgagcttca ccgagaactg gtccggcgcg agcggcgcac
     5401 cctggccgag cacctggacg cggcaagggc cgagcaacaa ctggatcgtc gatatcaaca
     5461 acaaccaggg acacatcaag aggaacagcg tcaacgtctc gtcgggcgtg ttctacctta
     5521 ataacgctag cgcccaggac gcggacatct ccgtcaagat caagtccaac ggcagcggaa
     5581 ttgtgaccgg cgtcgtcgcc cgccgttccg acagcgatcc cgatacctac ttcctgctgg
     5641 ccttcgacag cggtgacctg aacatctacg ccgtcgtcga tggcgcgaag tacctgctcg
     5701 accgcgacga ctacatcaac acgggcagcg gcttcaactg gatgcgattc ctggtcgaga
     5761 gcaatcccga cggcagcgtg cgcctccgcg gccggtactg ggccgacggc aacaccgagc
     5821 ccagcggctg gcacgtcgag gtccccgcgc tcagccaatt cccacagctc gccggcaaga
     5881 gcggccgctc ggggttgcat atcgatacgc agggcggctc gttccgcgaa gtgtggtatg
     5941 acagcttcac cgccaacacc aacctcgcgc cggttgtaac cgcctcggtc gaaccctttc
     6001 cggtcggctc cgccgtcggc acggccaatc tcgacggaac catcgccgac gacggagtcg
     6061 cgggccccct gaccgcgacc tggacgaaaa tcagcggccc gggctcggtc acgtttgcca
     6121 acaagaatgc ggtggacacc acggccgcct tttcgcttaa gggcacctac gtcgtacggc
     6181 tgaccgccaa cgacggcaat aagtccgcgt atgccgacgc gatcgtcaac gtcgtgcagg
     6241 agagcttcac gggcgcggac ggcagtgcct ggcccagcca gtgggtccag cagaacgggg
     6301 ccggggccgc gaccgcggac attcaagccg gccagggacg agtgaccaac ggcacgaccg
     6361 cgatcaacaa cgtctcgctg ttcaatagca cgacccaggc cgagaacgtg gacctgtcgg
     6421 tcagattccg cgtcgattcg aacctggtga ttccgggatt tcgagcccgc cgggcggaca
     6481 tgagagccaa tacgttctac tccgcctaca cactgcccgg aaccagcggg aacgtcggca
     6541 ttcaggcggt ggtgcggggt gtggcgacga cgctggcgac gggcgacttt ccgctcaccg
     6601 ccaacgcgga ctactacatg cgcttgcagg tggaaaccaa cgccgtggga acgacggacc
     6661 tgcgggtgaa gctctgggac gcggcggatg ccgagcccgg tgcctggacg atgcagctga
     6721 ctagcggcac gccggagctg caacgcgtcg ccgggcgttt tgggatcagc actggcttct
     6781 actacgctcc ctcgaagacg gcgtattacg acgatttcaa ggcgatcgcc caggtcaagg
     6841 agaacgcggc gcccaccgtg gctgcgatcg cccatctcga tcccgcggcg gcagtctgga
     6901 cggtcaatct caatggcacc gtcatcgacg accgccgccc cgccaactcg ctgaacacga
     6961 cgtggaccaa ggtcagcggc cccggcaccg tcgcgttcgg caatgccgct gccgtggaca
     7021 cgaccgccac gttttcgtct tcgggcgcct acgtgctgcg gcttaccgcc aacgacggcg
     7081 cccggtccaa ggccaaggac gtcgccatca acgtggtcga agagacgttc agtgccgccg
     7141 acggcagtcc ctggtcgtcg caatggaccc gccaacagat cggcggcacc gcgccggccg
     7201 cgaccgtgca gggaaacgaa gggcagatcg cgaacgcggg cggcggtttt tacggcaacg
     7261 tgctgatggt caacaatgcg gcggccagcg agaacgtgga tcaatcggtc aagttccgcc
     7321 tgaacggcga cttcgcgtgg accagcctga ttgcccgccg ggacgacgcg aatccagaca
     7381 cctggtacgc cgcggaaatc aacgtcaagc cgggcgggac gggcgcgctg acgatccaga
     7441 aggtcgtcga tggcgcggcg acggtgctgg gcacggcgaa cttcgcggtc gcgcccgaca
     7501 ccaactacct gatgcggttc caggtaacga gcaccaggac aggcaagacc gacctgcgga
     7561 tcaaggtgtg ggccgagggg agttccgagc cgtcggcgtg gaccctcgcg gtgctgggcg
     7621 acgcgacggc gcagttgcag ggcgtcgccg gccgcgtcgg catccgcacg ctctattatc
     7681 tggcccctcc cagccggacg gcgtggtacg acgattaccg ggctctgctc ttgggataat
     7741 gcgaagccga tgaggagatt acgtatgcgg atgctcgttg caattctgct tgtgctcggc
     7801 tgtaggacgg tccaggcgga taccttcgct tacgtctcgc tggccaagga caagaagatt
     7861 gccgtctaca acgtcgatcc atcgacggga gcactggcgc acgtggccga cgcgccctgc
     7921 ggaggagagc cggctgcact ggtcgcgaat ccgacccgaa cgtcgctctt cgcgtcgctg
     7981 cggcccgagg gcaagctggt cgcgtttcgc atcgatccgc ggacgggaac gctggcccat
     8041 ctgaatacga tcgacgccgg catcgatccg gctcatcttt ccaccgacaa gcaagggcgg
     8101 ttcctgctcg cggcctatta cgtggcggcc aaggtcacgg tccatgcgat cgaagctgac
     8161 ggctcgctgc ggccgcagcc ggtcgagagc gtgcccaccg ccgaaaaggc gcacgcgatt
     8221 ctcctcgatc ccgccaaccg ctttgccttc gttccgcaca ccgggcccaa cgcgatcttt
     8281 caattcgcct ttgatgccaa gaccggcggg ctgaaaccgg gaagtcccgc ccggctggca
     8341 acgccgccca acactggacc caggcacatc gtttttcacc cgcgattgga catggcttac
     8401 gtcagcaacg agcaaggcgg cagcgtgacc gcctggcgtt acgacaaatc cgccggctcc
     8461 ctggcggcag tgcaaactat ctccacgctg cctgccagct atggccagcg gaatgcttgc
     8521 gccgagatca agctccatcc cacgggtacg tttctctatg tcgccaaccg cggccacgac
     8581 agcatcgccc gctttcgcgt cgacggccgc agcggcgaat tgaccgccct cggccagact
     8641 tcgacggaga agaccccgcg gtctttcgac ctcgatcccg ccggcaagtt tctctacgtc
     8701 tgcggcgagg ccaccgaccg catcgccggg taccgcgtga gccctgactc tggcgatctg
     8761 gctccagtcg tgagcgggcc cgtgggcaag atgccctggt ggatcttgat ggtggacgtg
     8821 gcgcgctaag cttggcgact ggcgtgttcc agcggagcct gggcaagtaa aacaggtcca
     8881 atcatgccga gaactctgtc gctggcgacc acatgtgcac tcctcgccgc gatcgccgcc
     8941 gggcaggagc ggcgccgcta aactacgttg catgcttgcc gttgcagcac agactggttc
     9001 cacgcggccg ctcctggtcg aggcgccaaa tccgcgcgct cctggtccag g
//
LOCUS       DASRJB010000936         8112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_841834, whole genome shotgun sequence.
ACCESSION   DASRJB010000936 DASRJB010000000
VERSION     DASRJB010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841834"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFB80_24770"
     CDS             <1..384
                     /locus_tag="VFB80_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37077.1"
                     /translation="AKVKVVLVCLDHGKNDPSPHVPYKLVPIESYAKSNEVTEVVKML
                     ARGELDQHSAQAAAWHLQNGLTWEELVKKIGVKHLNGSIEPYFTGIHLQRALAATKVA
                     AQRAEKASQEKAPVKSASESIAGQD"
     gene            complement(493..1995)
                     /locus_tag="VFB80_24775"
     CDS             complement(493..1995)
                     /locus_tag="VFB80_24775"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZN37078.1"
                     /translation="MSAFEELCASAREAALLESIEGLLGWDERTYMPPAAGEYRAEQM
                     THLAGVIHKQRTSPRLGELLGELTGGPLAKDPHSDAGATIRQLKRDYDKRVKLPQSLV
                     EELTRASVLGQQAWVKARQDNDFPAFAPHAERLFALKRQQAACLGYADQAYDALLDDY
                     EPNAKTKDVAQVLAGLRAELVPLVQAIMQSGRTAPLEILQREYPPSAQEAFGKAAAAA
                     IGFEFERGRLDVTHHPFCSGLGPHDCRITTRYDQRFFNTSFFGILHEAGHGIYDLGLR
                     PDQFGLPPGTYVSLGIHESQSRMWENLVGRSRAFWQHFFPQWQAAFPAAAAGVKFDDF
                     FWAINHVAPSLIRVEADEATYNLHIIVRFELEQALLDQQLAVGDLPAAWREKYRQYLG
                     IEPETDADGSLQDIHWSAGLIGYFPTYSLGNLYAAQFFEQADRDLGGLAALFARGEFQ
                     PLKRWLNEKIHHRGQSYSSAELVQLVTGQPMSHAPLIRHLRGKLGPLYGI"
     gene            complement(2159..2413)
                     /locus_tag="VFB80_24780"
     CDS             complement(2159..2413)
                     /locus_tag="VFB80_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37079.1"
                     /translation="MPFARPWPPAALTLFLFTTGCAVLFAGCATTPSEYWQALKGEGF
                     PEWNENMAAGSRGDPSRAKPSGFFTDRRSEQIEQNLGGGF"
     gene            2676..4091
                     /locus_tag="VFB80_24785"
     CDS             2676..4091
                     /locus_tag="VFB80_24785"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN37080.1"
                     /translation="MTSQRHSDLGCPEFRQLARTSRRSVLQAGAAGLLGISLAELLAA
                     QAAAGNSASDKPGFGRAKRCIFLFMWGGPSQLDTFDPKPQAPDNIRGPFKPIATKVPG
                     LQISEHFKQLAGLMDKVALIRSLGHDDPAHLSSGHTTVTGHLAPVVRSDATPPSPHDT
                     PHLGSVLAKLRPSGEALPPFVLLPWIAYHPAAPGGQAPGQHGGWLGPQYDPLLATGDP
                     NDANWKLPALALQPGITPELLGSRFELLKQIDQQRAEIDALAARSAITGHQARALSLL
                     SSPRVRGAFDLAQEPAEVRERFGRNIHGQCVLLARRLIEHGVNLVCVNWHNDGRSFWD
                     THGNNFNRLKNDLIPPADRALSALLTDLSDRGLLDDTIVAWVGEFGRKPEISTNNSGR
                     EHWPYCYSGLLAGGGIAGGAVYGSSDKQAAYPATNPVSPHDYAATLLHALGIPESTVL
                     HDSANRPRHVYAGRPIEPLFS"
     gene            4104..5474
                     /locus_tag="VFB80_24790"
     CDS             4104..5474
                     /locus_tag="VFB80_24790"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN37081.1"
                     /translation="MRSTTSLSRRHMLQIGALTPLGLSLSGLLAGEARGASGGELAAS
                     FGKAKRCLLLFMWGGPAHQDLWDLKPQAPDNIRGEFQPIATKVPGIQIGELLPNIAQH
                     TDKLALIRSVTHTDNNHSTGAHWMLTGHKHRVSAENFGASPSDFPHLGSVVSKLRPSR
                     QSLPSFVALPERIGTTAGAITPGQNGGILGSQYDPFTIDEHPDKPDFKIDMLAQRAGL
                     DDGRVAQRQHLLATLNAQRAELDASRRVDALNGYFQRAADLVTSPAARAAFDLSQETD
                     KERDRYGRKTFGQSLLLARRLLVAGVRLVTVYWHRDMPGVDTTWDTHGQNFKQLKERL
                     VPQVDQPIATLLADLQDRGLLDETLVVWSSEFGRTPKINGNAGRDHWGPCNSIWLAGG
                     GVRGGQVYGSSDKQAAYPADNPVGPADVTATIFHLLGIDPHTQIHDREGRPLAISHGR
                     VIEGLF"
     gene            5478..5750
                     /locus_tag="VFB80_24795"
     CDS             5478..5750
                     /locus_tag="VFB80_24795"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZN37082.1"
                     /translation="MADEKLPPPSLSLLIASLGTQAMVALGQVPNPLDGKTEVRLDLA
                     KHVIEMLAILEQKTKGNLDADEVKMLESVLHQLRMAFVAAAPKPKP"
     gene            complement(5767..6834)
                     /locus_tag="VFB80_24800"
     CDS             complement(5767..6834)
                     /locus_tag="VFB80_24800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN37083.1"
                     /translation="MNIECDETTAVRPGEELDLARLEGYLRERLPECAGPLAALQFPH
                     GHSNLTYLLRAGASEFVLRRPPFGNQVQTAHDMGREYRVLAKLSAVFPEAPRPYLFCD
                     DSGVLGAPFYVMERRRGLILRKSLPPGLSLTPETAGRLGQSLIETLARLHAVNYQAAG
                     LADLGKPAGYVARQVTGWIKRYEGARTDDVKSMERVAQWLTEHLPAESGVAVIHNDYK
                     YDNVVLDPAKMTRIVAVLDWEMATLGDPLMDLGTTLGYWVEGGDPQPLQQAAIGPTML
                     RGSPTRRELVECYERATGRDVPQPLFYYAFGLFKIAVIIQQIYARLVRGHTRDPRFAR
                     MNEIALVMSHQAERAIAGGVI"
     gene            complement(6837..7553)
                     /locus_tag="VFB80_24805"
     CDS             complement(6837..7553)
                     /locus_tag="VFB80_24805"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN37084.1"
                     /translation="MSSLTLVRHAQASFFADHYDQLSPLGENQARLLGEWWAERRVVP
                     SEVYVGPRQRHRQTAAAVAAVYVRAGLPFPQPVELADLDEYDLSGILRILAPALAREN
                     EDFAVLHQRQRHGASQREQARSFQRMFEPLLVHWQAAAGLEGLESWAAFQLRIRRCLK
                     QLTERPGRGRRIFAFTSGGFIGAAAQWVLAAPDRTALELNWRIHNTALTQFVFSGERI
                     TLDAFNSIAHLPDPELATYR"
     gene            complement(7565..>8112)
                     /locus_tag="VFB80_24810"
     CDS             complement(7565..>8112)
                     /locus_tag="VFB80_24810"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37085.1"
                     /translation="WIISGGWDGTTRIWDAASGEARARLTGTGGVDALSYAADSRLLA
                     ICANGKEVQLFELRLGEPSAEQLAQLRAILARLDDDRIEVRDLASAELVKLGIAVEPE
                     LVRVAAESSSAEVRIRARRARQAILTVPPGGIGGHEGEIHSAVFSPNGQLLASCSDDG
                     TVRLWDVPKRTELARIVPAMR"
BASE COUNT         1404 a         2757 c         2658 g         1293 t
ORIGIN      
        1 gcgaaggtaa aggttgtcct ggtctgcctc gatcacggca agaacgatcc cagcccgcat
       61 gtgccctaca agctcgtgcc gatcgaatcc tacgccaaga gcaacgaggt aaccgaagtc
      121 gtgaagatgc tcgcccgcgg cgagctcgac cagcactcgg cgcaggccgc cgcgtggcac
      181 ctgcagaacg gcctgacctg ggaagagctg gtgaagaaga ttggcgtaaa acacctcaac
      241 ggcagcatcg agccctattt caccgggatc cacctgcagc gggcgctggc tgcgaccaag
      301 gtcgccgccc agcgggccga gaaggcctcg caggaaaagg cgccggtcaa atcggccagc
      361 gaatcgatcg cgggacagga ttgacgctga gtctgtactg caaaaccatg ggcgttctca
      421 ggtcaacgag aacgcccttt ttctttgccg tcgcactgcg cctcggctgt gggagcgtgg
      481 ccgccgctga atctagatgc cgtagagcgg gcccagcttg ccgcggagat gccggatcag
      541 gggcgcgtgc gacatcggct ggccggtgac cagctgcacg agctcggctg acgaatagga
      601 ctggccgcgg tggtggattt tctcgttcag ccaccgcttc agcggctgaa actcgccgcg
      661 ggcgaacagc gcggccaggc cgcccaggtc gcggtcggcc tgttcgaaga actgcgcggc
      721 gtagagattg cccagcgaat aagtcgggaa gtagccgatc agccccgcgg accagtgaat
      781 gtcctgcagg ctgccgtcgg cgtcggtttc cggctcgatg cccaggtact ggcggtattt
      841 ctcgcgccag gcggccggca ggtcgccgac ggccagttgc tggtcgagca gggcctgctc
      901 cagttcgaaa cggacgatga tgtgcaggtt gtacgtagcc tcgtcggcct cgacacggat
      961 cagcgagggc gcgacgtggt tgatcgccca gaaaaagtcg tcgaatttca cgccggcggc
     1021 cgcggcgggg aaggccgctt gccattgcgg aaagaaatgc tgccagaacg cccgcgagcg
     1081 gccgacgagg ttctcccaca ttcgcgattg cgactcgtga atccccagcg acacgtaggt
     1141 gcctggcggc aggccgaact ggtcgggccg cagccccagg tcgtagatgc cgtgccccgc
     1201 ctcgtgcagg atgccgaaga acgaggtgtt gaaaaaccgc tggtcgtagc gggtggtgat
     1261 gcggcaatcg tgcgggccca ggccgctgca aaacgggtgg tgcgtcacgt ccagccgccc
     1321 gcgctcgaat tcgaacccga tcgccgcggc ggccgccttg ccgaacgcct cttgagccga
     1381 cggcggatat tcccgctgca aaatctccag cggtgccgtg cggcccgatt gcatgatcgc
     1441 ctgcaccagc ggcaccagct ccgcccgcag cccggcgagc acctgggcca cgtccttcgt
     1501 cttcgcgttc ggctcgtaat cgtccagcaa agcgtcgtac gcctgatccg cgtaccccag
     1561 gcaggcggcc tgctgccgct tgagggcgaa gagccgctcg gcgtgcgggg cgaaggcggg
     1621 aaagtcattg tcctggcggg ctttgaccca ggcctgttgg cccaggacgc tggcccgcgt
     1681 caattcctcg acgagcgatt gcggcagctt cacccgcttg tcgtagtctc gcttcagctg
     1741 gcggatcgtc gcgccggcgt cgctgtgcgg gtccttcgcc agcgggccgc cggtcaattc
     1801 gcccaatagt tcgcccagcc gcgggctggt ccgctgcttg tggatcacgc ccgccaggtg
     1861 cgtcatttgc tcggcgcgat attcgccggc ggcgggaggc atatacgttc gctcgtccca
     1921 gccgagcagc ccctcgatcg attccaggag cgcggcctcg cgggccgagg cacagagttc
     1981 ctcaaaggcg gacatcggtg aaatgtcgaa ggacgaaaat caaaggacga aagaagcacg
     2041 aagtttgaat accgagttat acactggttc ccaggctcct gcctcgcggg gcgctgacga
     2101 agcgagagcc tcggggaagt gcgctcccgg aaggaccctg ggaacgagtg gcgtgacttc
     2161 aaaagccccc gcccaggttc tgttcgatct gctcgctgcg gcggtcggtg aagaagccgc
     2221 tgggtttggc ccgggaggga tcgccgcgcg agccggcggc catgttctcg ttccactcgg
     2281 gaaagccttc gcccttgagg gcctgccagt attcgctcgg cgtcgtggcg cagcccgcaa
     2341 acaacaccgc gcagcccgtt gtaaatagaa acaaagtcag cgcagccggc ggccaaggac
     2401 gtgcgaatgg catagagatc tccaagagca gcgtggcggc ggaatatcgc aggccggcca
     2461 caccgcgccc aggcaaactg gagaaacccg ctagctgcca tccggaaggg gtcgggagtc
     2521 tttgtcgtca tcggctttcc atgttcacaa tccttgccga caaagactcc cgaccccctg
     2581 gcgccgcctg aacctttcct tccccgcagc cgaaagctac gatagttctc gcatcgaagg
     2641 cggttgatgt tcctcgtttt ccggctgcta tctggatgac ttcccagcgc cactccgacc
     2701 tcggctgccc cgagttccgc cagctcgcgc gaactagccg ccgcagcgtg cttcaggcgg
     2761 gagcggctgg tctcttgggc atttcgctgg ccgaactgct ggcggcgcaa gcggcggccg
     2821 gcaactcggc gagcgacaag ccgggcttcg gccgggccaa gcggtgcatc ttcctgttca
     2881 tgtggggcgg gccgagccag ctcgacacat tcgatcccaa gccgcaagcg cccgacaaca
     2941 tccgcgggcc atttaagccg atcgccacga aggtgcccgg actgcagatc agtgaacact
     3001 tcaagcaact cgcgggcttg atggacaagg tcgcgctgat ccgctcgctg gggcacgacg
     3061 atccggcgca cctttccagc ggccacacga cggtgaccgg gcatctggcg cccgtcgtcc
     3121 gcagcgacgc aacgccgccg agcccgcacg acacgccgca cctgggctcg gtgttggcga
     3181 agctgcggcc cagcggcgag gcgctgccgc cgttcgtgct cctgccctgg atcgcctatc
     3241 atccggccgc gcccggcggg caagcgccgg ggcagcacgg cggctggctc ggaccccaat
     3301 acgacccgct tctggccacg ggcgacccca acgacgcgaa ttggaagctt cccgcgctgg
     3361 ccttgcagcc gggcatcacg ccggagttgc tgggcagccg cttcgagctt ttgaagcaga
     3421 tcgaccagca gcgggcggag atcgacgcgc tggcggcccg ctcggcgatc accggccatc
     3481 aagcgcgggc gctgagcctg ctcagttcgc cgcgggttcg cggggcgttc gacctggctc
     3541 aggagccggc ggaagtccgc gagcgttttg gccgcaacat ccacggacag tgcgtgctct
     3601 tggcccgccg gctaatcgag cacggcgtga acctggtctg cgtcaactgg cataacgacg
     3661 gccgctcgtt ctgggacacg cacgggaaca acttcaaccg gctgaagaac gacctgattc
     3721 ccccggccga ccgcgccttg tccgcgctcc tcaccgatct ttccgaccgc gggctgctgg
     3781 acgacacgat cgtggcctgg gtcggcgagt ttggccgcaa gccggagatc agcaccaaca
     3841 atagtggccg cgagcactgg ccctattgct acagcggcct cttggccggc ggcggcatcg
     3901 cgggcggcgc ggtctatggc agcagcgaca agcaggccgc ctatccggcc accaacccgg
     3961 tgtcgccgca cgactacgcc gccacgctgc tgcacgcgct gggcattcca gaaagcaccg
     4021 tgctgcacga ctccgccaat cgcccgcgcc acgtttatgc cgggcggccg attgaaccgc
     4081 tgtttagcta gagtcgatcg acgatgcgat ccacaaccag tctctcccgc cgccacatgc
     4141 ttcaaatcgg cgcgctgacg ccgctggggc tgtcgctgag cgggctcttg gcgggcgaag
     4201 cgcggggggc gagcggcggc gagctggcgg cctcgttcgg caaggccaag cgctgcttgc
     4261 tgctcttcat gtggggcggg ccggcccatc aggacttgtg ggacctgaag ccgcaagcgc
     4321 ccgacaacat tcgcggcgag tttcagccga tcgcgaccaa ggttccgggg attcagatcg
     4381 gcgaattgct gcccaacatc gcccagcaca ctgacaagct ggccctgatt cgcagtgtca
     4441 cgcacacgga caacaaccac tcgaccggcg cacactggat gctcaccggc cacaagcacc
     4501 gcgtcagcgc cgagaacttt ggcgcatcgc cgagcgattt tccgcacctg ggctcggtcg
     4561 tcagcaagct gcggccgagc cggcagagcc tgccgtcgtt cgtcgccctg cccgagcgga
     4621 tcggcaccac cgccggcgcg atcacgccgg ggcaaaacgg cggcatcctc ggcagccagt
     4681 acgatccctt cacgattgac gagcatcccg acaagcccga cttcaagatc gacatgctgg
     4741 cccagcgggc cggcctcgac gacggccgcg tcgcccagcg gcagcacctg ctggccacgc
     4801 tgaatgccca gcgggccgag ctcgatgcca gccgccgcgt cgatgcgctg aacggctact
     4861 tccagcgggc cgccgacctg gtgacttcgc cggcggcccg cgccgcgttc gacctgtcgc
     4921 aggaaaccga caaagagcgc gaccgctacg gccgcaaaac cttcggccag agcctgctct
     4981 tggcccgccg gctgctggtc gcgggggtcc gcctcgtcac ggtctattgg caccgcgaca
     5041 tgcccggcgt cgataccacg tgggacacgc acggccagaa tttcaagcag ctcaaggagc
     5101 ggctggtgcc gcaggtcgat cagccgatcg ccacgctgct ggcggacttg caggaccgcg
     5161 gcctcctgga cgagacgctg gtcgtctggt cgagcgagtt cggccgcacg ccgaagatca
     5221 acgggaatgc cggccgcgac cactgggggc cgtgcaactc catctggctg gccggcggcg
     5281 gcgtccgcgg cgggcaggtc tatgggtcgt ccgacaagca ggcggcctat cccgccgaca
     5341 atcccgtcgg ccccgccgac gtgaccgcga cgatctttca tctgctgggg atcgatccgc
     5401 acacgcagat tcacgaccgc gagggccggc cgctggcgat ctcgcacggg cgggtgatcg
     5461 aagggctgtt ttgatccatg gccgacgaaa agctcccgcc gccgtcgttg tcgctgctga
     5521 tcgcctcgct cggcacgcag gcgatggtcg cactcggcca ggttcccaat ccgctcgatg
     5581 gcaagactga agtgcggctg gacctggcga agcacgtcat cgagatgctg gcgatcctgg
     5641 agcaaaagac caagggcaat ctcgacgcgg acgaagtgaa aatgctcgaa agcgtgctgc
     5701 atcagctgcg gatggccttt gtcgcggccg cccctaaacc gaagccttag cgctccgtcg
     5761 cgacgatcaa atgactccgc cggcgatggc ccgctcggcc tgatggctca tgaccagggc
     5821 gatctcattc atgcgcgcga agcgtggatc gcgggtgtgg ccgcggacga ggcgggcgta
     5881 aatctgctgg atgattacgg cgatcttgaa caggccgaag gcgtaataga acagcggctg
     5941 gggcacgtcg cggcccgtgg cccgctcgta gcattcgacc agttcccgcc gggtcgggct
     6001 gccgcggagc attgtcggcc cgatggcggc ctgctgcaga ggctgcggat ctcccccttc
     6061 gacccagtag cccagcgtcg tgcccagatc catcagcgga tcgccgagcg tggccatctc
     6121 ccaatccagc accgcgacga tccgcgtcat cttcgccggg tcgagcacca cgttgtcgta
     6181 cttgtagtcg ttgtggatca ccgcgactcc cgactccgca ggcaggtgct cggtcagcca
     6241 ctgggccacg cgctccatcg acttaacgtc gtcggtgcgg gcgccctcgt agcgcttgat
     6301 ccagcccgtg acttgccggg ccacatagcc ggcgggcttg cccagatcag ccaggccagc
     6361 agcctggtag ttgaccgcgt gcagccgggc gagcgtttcg atgagcgact ggccgagccg
     6421 tcccgccgtt tccggcgtca gcgacagtcc cggcggcagg ctctttcgca ggatcaggcc
     6481 gcggcggcgc tccatcacat aaaacggcgc gcccagcacg ccgctgtcgt cgcaaaacag
     6541 gtacggccgc ggcgcttcgg gaaacacggc cgacagcttc gccagcacgc ggtactcgcg
     6601 gcccatgtcg tgcgcggtct gcacctggtt gccgaaggga ggccggcgga gcacgaattc
     6661 agaggctccg gcacggagca agtacgtcag gttggagtgc ccgtgcggaa actgcagggc
     6721 cgccagcgga ccagcgcact ctggcaggcg ctcgcgcaag taaccttcca gccgagcgag
     6781 gtcgagttcc tcgccgggac ggaccgccgt cgtctcatcg cattcgatat tcatgattag
     6841 cggtaagttg ccagctccgg atcaggcaga tgcgcgatgc tgttgaaagc atccagcgtg
     6901 atccgctcgc cggagaatac gaactgggtg agtgcagtgt tgtgaatccg ccagttgagc
     6961 tccagcgccg tccggtccgg cgcggccagc acccattgcg ccgccgcgcc gatgaatccg
     7021 cccgaggtga acgcaaaaat ccgtcgcccg cggccgggcc gctcagtcaa ttgcttgagg
     7081 cagcggcgga tccggagctg aaatgccgcc caggattcca gcccttccag ccccgcggcc
     7141 gcctgccagt gaacgagcag cggctcgaac atccgctgga agctgcgggc ctgctcgcgc
     7201 tggctcgcgc cgtgccgctg ccgctgatgc agcacggcaa agtcctcgtt ctcgcgagcc
     7261 aatgcgggcg cgagaatccg gaggattccg ctcaggtcat attcgtccaa gtccgccagc
     7321 tccaccggct ggggaaacgg caggccggcc cgcacgtaga ccgctgccac tgccgccgcc
     7381 gtctgccggt gccgctgccg cggcccgaca tagacctcgc tggggaccac gcgccgctcg
     7441 gcccaccact cgccgagcaa gcgggcctgg ttctcgccca gcggcgagag ctggtcgtaa
     7501 tggtcggcga agaacgaagc ctgcgcatgc cggactagcg ttagcgaact catgcgcgtc
     7561 tggttcacct catcgccggt acgatccggg ccagttccgt gcgcttgggc acatcccaca
     7621 ggcggaccgt gccatcgtcg ctgcagctgg ccaatagctg gccattcggc gagaagacgg
     7681 cagaatggat ttctccctca tgaccgccga tgccgcccgg cggcaccgtc aggatcgcct
     7741 gccgggcgcg gcgggcgcga attcgcactt ccgccgagga ggattcagcc gccacacgca
     7801 caagctccgg ttccacggcg attcccagct tgaccagctc ggcgctggcc aggtcgcgga
     7861 cctcgatccg atcgtcgtcc agccgcgcca ggatcgcgcg gagctgcgcc aactgctcgg
     7921 cggagggctc gccaagacgc aactcgaaca gctgcacttc cttgccgttg gcacaaatcg
     7981 ccagcagccg cgaatcggcg gcataggaaa gcgcgtccac gccgccggtg cccgtgagcc
     8041 gagcgcgggc ctcgccgctg gcggcgtccc agatgcgcgt ggtgccgtcc cagccgccac
     8101 tgatgatcca gc
//
LOCUS       DASRJB010000937         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3097910, whole genome shotgun sequence.
ACCESSION   DASRJB010000937 DASRJB010000000
VERSION     DASRJB010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097910"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFB80_24815"
     CDS             complement(<1..651)
                     /locus_tag="VFB80_24815"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN37086.1"
                     /translation="MAQTAAAHRMQQLRLAIVAPRFWPLVEDAPQHLLQLAESLIAAG
                     HAVDVVTPLWKRSWPRAMQVGPVPLVRLRGSPRTALGTVRWMYSLAGWLREQRPAGML
                     VGGLRHEAYVALGMPPKIRPRVVLVAGEDDLAWQRTAAFGSRIAARCRQAPAIVAASE
                     ELADGLASAGYSRERITVIPRRVPIPSPRSPAARDQARAALAAVNHDLVTTTTAPVA"
     gene            complement(676..1194)
                     /gene="hpt"
                     /locus_tag="VFB80_24820"
     CDS             complement(676..1194)
                     /gene="hpt"
                     /locus_tag="VFB80_24820"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZN37087.1"
                     /translation="MRPLVSAEQIQASIDRLAAEIRAREAGRPLTVIAVMTGSIIFLA
                     DLIRKLDMPLRVGVIQARSYAGTQRGSLSVNADMLPDVAGRDVLVIDDIFDTGHTLQE
                     VLAFLDELGARSIRTAVLVLKRGKQLVSLRPDYVGFEIPDVFVVGYGLDYNDGYRNLP
                     YLAALEPADIEA"
     gene            complement(1297..>2693)
                     /gene="hpnE"
                     /locus_tag="VFB80_24825"
     CDS             complement(1297..>2693)
                     /gene="hpnE"
                     /locus_tag="VFB80_24825"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZN37088.1"
                     /translation="KTRVAVVGGGLAGLAAAAALVERGCSVELFEAKRTLGGRAGSYS
                     DRATGERIDHCQHVAMGCCTNFLDFSRRTGIADLFTRYQTLHFFGPDGRRCDFRPTGW
                     LPPPLHLASPLWHMNFLPIADKIAIARAMLGLLRLPAEDCAGGSTVLDWLRSQRQSAR
                     AIERFWQVVLVSALGESLDRASVAAARKVFLDGFLGHREACHVLVPRVSLGELYDQRV
                     ADWLRSRGAAIHLEMPVAEVTAAGQSVTGLRLAGGDSREFDLVILAVPWRRVGDLLSD
                     EMQNAIGVAQWADMPSAPISALHLWFDRELTELPHAVLVGRLSQWVFAHGRHYYQVVI
                     SASHDLAGRQRQAVLDEVLGDLRAVFPKAQGASLLRWQQITQPEAVFSVRPGLDEIRP
                     PQQTLVPNLLLAGDWTRTGWPATMEGAVRSGYLAAEALLARLGQPQQILAPDLPQGWL
                     LKRVQSSRFRVQSS"
BASE COUNT          459 a          918 c          926 g          390 t
ORIGIN      
        1 ggcgacggga gccgtggtcg tcgtcaccag gtcgtgattc accgcggcca gcgccgcgcg
       61 ggcctggtcg cgtgcggccg ggctccgtgg cgacggaatc ggcacgcgcc gcggaatcac
      121 ggtaatccgc tcgcgcgagt atccggcgct tgcgagcccg tcggccagtt cctcgctcgc
      181 ggcgacgatt gccggagcct ggcggcagcg ggcggcgatg cggctgccaa aggccgcggt
      241 ccgctgccag gcgaggtcgt cctcgcccgc cacgagcacc acccgcgggc ggattttcgg
      301 cggcatgccc agcgcgacgt aagcttcatg acgcagcccg ccgacgagca tgccggcggg
      361 cctctgctcg cgcagccatc ccgcgaggga gtacatccac cgcacggtgc ccagcgccgt
      421 gcggggcgaa ccgcgcaagc gcaccagcgg caccggcccg acctgcatcg ctcgcggcca
      481 agagcgtttc cagagcggcg ttaccacgtc gaccgcgtgg ccggcggcga tgagcgactc
      541 ggccagctgc agcaggtgct ggggcgcgtc ctcgaccagc ggccagaacc gtggcgcgac
      601 gatcgccagg cgaagctgct gcattcgatg agcggcggca gtctgcgcca tgtgtccagc
      661 gggcggctct ggcggctagg cttcgatgtc tgccggctca agggccgcca gatagggcag
      721 gttgcgatag ccgtcgttgt agtccaggcc gtaacccacg acaaacacgt cgggaatttc
      781 gaagccgacg tagtcgggcc gcagcgagac gagttgcttg ccccgcttga gcaccagcac
      841 cgccgtgcgg atcgagcggg cccccagttc gtcgaggaag gccagcactt cctgaagcgt
      901 atgccccgtg tcgaagatgt cgtcgattac cagcacgtcg cggcccgcca catcgggcag
      961 catgtcggcg ttgaccgaca gcgagccgcg ctgcgtaccg gcatagctgc gcgcctgaat
     1021 cacgccgacc cgcagcggca tgtcgagctt gcggatcagg tcggccagga agatgatgct
     1081 gcccgtcatc accgcgatca ccgtcagcgg ccgccccgcc tcgcgggcgc ggatctccgc
     1141 cgccagccgg tcgatcgaag cctggatttg ctcggcggag acgaggggac gcatggagag
     1201 ttcagggttt ggggttcagg gttcagaagt cggatgtcag agtcggtgat tgatccgtac
     1261 ttgttactcg ctactgagta cagagtacag aggcgatcag ctgctctgaa ccctgaacct
     1321 cgaactctga accctcttca gcagccagcc ctgcggcagg tcgggcgcga gaatctgctg
     1381 cggctggccc aggcgggcca gcagtgcttc ggcggcgaga taaccgctgc ggactgcgcc
     1441 ctccatcgtg gcgggccagc cggtgcgtgt ccaatccccg gccagcagca gattgggaac
     1501 gagggtttgc tgcggcgggc gaatttcgtc caggccgggg cgcacggaga agacggcttc
     1561 gggctgcgtg atctgctgcc accgcagcaa gctcgcgccc tgagccttgg gaaagacggc
     1621 tcgcaggtcg cccagcactt cgtcgagcac ggcctgccgc tgccggcccg ccagatcgtg
     1681 cgaggcgctg atgacgacct ggtaatagtg ccggccgtgg gcgaataccc actgcgacag
     1741 ccggcccacg agcaccgcgt ggggcaactc cgtcagttcg cgatcgaacc acaggtgcag
     1801 ggccgaaatc ggggccgacg gcatgtctgc ccactgcgcc acgccgatcg cattctgcat
     1861 ttcgtcgctg agcagatcgc ccacgcgccg ccaaggcacg gcgagaatga ccaggtcgaa
     1921 ttcgcgcgag tcgccgccgg ccaagcgcaa cccggtgacg ctttgccctg cggcggtgac
     1981 ttccgcaacg ggcatttcca agtgaatcgc tgccccccgg ctgcggagcc agtcggcgac
     2041 gcgctgatcg tacagctcac ccagcgaaac gcgggggacc agcacgtggc aggcctcgcg
     2101 gtggcccaga aagccgtcga gaaagacctt gcgggcagca gccaccgagg cccggtcgag
     2161 ggattcgccc aaggcactca ccagcaccac ctgccagaat cgctcgatcg cccgggcgct
     2221 ctgccgctgc gagcgaagcc agtccaagac ggtcgatccg cccgcgcagt cctctgcggg
     2281 cagccgcagc aggccgagca tggcccgggc gatcgcgatc ttgtcggcaa ttggcagaaa
     2341 gttcatatgc cacagcggac tggccaaatg gagcggcggc ggcagccagc ccgtcggccg
     2401 aaagtcgcag cggcggccat ctggaccgaa aaagtgcaga gtctggtatc gcgtgaagag
     2461 gtcggcgatc cccgtgcgtc gcgagaagtc gagaaagttc gtgcagcagc ccatcgccac
     2521 gtgctggcaa tggtcgatcc gctcaccggt agcgcggtcg ctgtaagacc cggcgcgccc
     2581 gccgagcgtg cgcttggcct cgaacagttc cacgctgcaa ccgcgctcga ccagcgccgc
     2641 cgcagccgcg agtccggcca ggccgccgcc gacgacggcc acgcgggttt tcg
//
LOCUS       DASRJB010000938        11872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1405273, whole genome shotgun sequence.
ACCESSION   DASRJB010000938 DASRJB010000000
VERSION     DASRJB010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405273"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFB80_24830"
     CDS             <1..270
                     /locus_tag="VFB80_24830"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZN37089.1"
                     /translation="NATCAVSCTGTGEQFIRNAVAYDLSARLKYLKQPLAEAVNEVLH
                     KTLRPSDGGLIALGADGTIVMDYSTGGMARAAADASGRREVKIGK"
     gene            complement(359..991)
                     /gene="upp"
                     /locus_tag="VFB80_24835"
     CDS             complement(359..991)
                     /gene="upp"
                     /locus_tag="VFB80_24835"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZN37090.1"
                     /translation="MPTTEVAHPLVKHHITRLRDKTTPPAEFRQLVQRLSVLLAYEAT
                     QDLELVPRELETPLVPMTGSALKQRIGLAPILRAGLAMVDPILNLIPSAEVWHLGLYR
                     DEATARPVEYYSKLPKTEPVEVALVVDPMLATGGSAMAALAALQRWGVKRLKVLSLIA
                     SQHGVSAVREQFPAADIFVCAIDAELNSRKFIVPGLGDAGDRIFNTLRGE"
     gene            complement(1106..1504)
                     /locus_tag="VFB80_24840"
     CDS             complement(1106..1504)
                     /locus_tag="VFB80_24840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZN37091.1"
                     /translation="MRVKSAKELEIYQLANEFAMRIFEISKRFPAEERYALTSQIRRS
                     SRSICLNLREAWAKRRYAAHFLSKLTDCDGENSETDSSLDFAHQCGYIDAEEHKALTA
                     MSASIGRMLGAMIQNYESFIQDSATHRHTD"
     gene            complement(1592..2758)
                     /locus_tag="VFB80_24845"
     CDS             complement(1592..2758)
                     /locus_tag="VFB80_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN37092.1"
                     /translation="MAAPPARADVFRLESGGQIEGEWLNHAEQPLTRYLVRTADGLTL
                     TLGLAQVKEAVRQSPAEIEYRRLVPLAPDTLDGQWSLAEWCRKHGLTRQREAHLRRMI
                     EIDPNHQQARYAMGYQFLKGQWISRSDFRRQEGYEFYKGKWRTPQEIEILETRARVEL
                     AEKDWLLKLRRWRANLDDPDKAKFGYESLQAVSDPVAVRPLGQLFGQERMRRVKMLYA
                     DILAKINTPDAVAILVDRTLADFDEEILYYCLDKLAELQPPHVADPFVTALKDADNGK
                     VNRGAIALARLRDPSTISPLIDALVTTHTRVLPGRAGPNATTTAFSDGGTFMRQNEGP
                     QVQIVHMQNQPVLDALARLTGESFGFDKTRWRYWHSQEQIAQESGKPAFDARRQ"
     gene            2956..3465
                     /locus_tag="VFB80_24850"
     CDS             2956..3465
                     /locus_tag="VFB80_24850"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZN37093.1"
                     /translation="MSATHEEHKSQGPPSVRCAVITVSDTRTLETDTGGQAVIAHLTA
                     AGHVVVQREIIPDEPARMRPLLETLSRRDDLDAILLTGGTGITSRDQTFETVSGLLTK
                     PLPGYGELFRMLSFQEIGPAAILSRAVGGLIGRKIVLTMPGSPAAVRLAMEKIIVPEL
                     PHLVREARR"
     gene            3469..3960
                     /locus_tag="VFB80_24855"
     CDS             3469..3960
                     /locus_tag="VFB80_24855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37094.1"
                     /translation="MPLSENPYESPRIPNAVAAPQAPPPGHHGWKFVLLGIAIACGIV
                     SLAPWLGVLFAIFSAPVFLRYFVFGWKRPQMTPWDYAGSRAAGAFGAIGFGLSILAAV
                     SGAFFGTCNVAGWSVAFSTLATNIHYERVIAAAVAIGVMAGLAAAVTALVWLVRFYYP
                     PRA"
     gene            4166..4846
                     /gene="phoU"
                     /locus_tag="VFB80_24860"
     CDS             4166..4846
                     /gene="phoU"
                     /locus_tag="VFB80_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZN37095.1"
                     /translation="MSKHLHRDLENLNTELLSISAMVEEMIDKATLALSERRYELADE
                     VVQSDEYVDEHEVHVEEECLKMLALHQPVAVDLRRIATVLKANADLERIADLAVSIAQ
                     RARAMHEFPAFPIPEPLRPMVLLATQMVRGAMNAFVNTDNAAARRIIAMDKTVDQYNR
                     EIIDELQKLMQKRPEAVPAAVNCFSAVRHIERIADHATNIAEDVIYLVEGDIVRHRMD
                     QPTSETPK"
     gene            4892..5599
                     /locus_tag="VFB80_24865"
     CDS             4892..5599
                     /locus_tag="VFB80_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN37096.1"
                     /translation="MAKTKVLVIEDDRALAEVLTYNLKQAGYDVLLATDGQDGLLQAQ
                     IKSPDIVLLDLMLPVIDGLDVCRRLRAGTGTKDLLIVMVTAKAEESDELVGFSLGADD
                     YVTKPFSVKVLLERIKALRRRRQTEGVTDEVTSKLGVIVDRRRHQATAEGKPLELTRS
                     EFRLLDTLIRQPGRVFHRSELIDAALGEDTMVMERTIDVHIRALRRKLADRADVIETV
                     RGVGYRFRDTEPICEPA"
     gene            5681..6574
                     /locus_tag="VFB80_24870"
     CDS             5681..6574
                     /locus_tag="VFB80_24870"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN37097.1"
                     /translation="MKLLRCVVSAAGTILCIAAASALAADVPSVVFLWPAGAATLQGG
                     NEKEITTPADVKPGERINSIRNVHNPSLEVHLPPADKAVGTGVIVAPGGGHRQLVWGS
                     EGTDIAQFLNEIGVAAFILKYRLEQTPNYKYTVEGEALADTQRAIRIVRARAKEWGVQ
                     QNRIGVLGFSAGGALAALADIHFDRGKADSSDPIEQQSCRPDFVGLVYPGWRPMDLTA
                     PKDAAPAFLTSAGLDDASHARATVDFHQSLFKAGVQTELHIYARGGHGGAIKPRDGIP
                     FGKWHIRFQEWLADLGMLKQP"
     gene            complement(6571..7461)
                     /locus_tag="VFB80_24875"
     CDS             complement(6571..7461)
                     /locus_tag="VFB80_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37098.1"
                     /translation="MIPLFEHKAAGQVLFLLSGACLVKFNVQSCRRLAGFVMGSVAIG
                     CCLSLARAQESEGARRGGEGAGERDRGGGEERRGGEDRDAGRGPQERAPEGRGAPEGR
                     RPEGRGPEGGPGRTPMARPGVGGGGGFGGGFGGAQGGQLGGVRGFGGGGFGGRGGGEG
                     ALDERISRLEQKLDQILAELRELKNQRPGGRPATARGGRPQGPPAGPGGRGGPRGTRG
                     PGEFGRMGGFGPGFGPPANRPGFGGDFGRSPRPGGPGGPGGEFRGPPRGEREESRERE
                     GRGEREDRDERIGRGERGRD"
     gene            complement(7547..8203)
                     /locus_tag="VFB80_24880"
     CDS             complement(7547..8203)
                     /locus_tag="VFB80_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37099.1"
                     /translation="MWRKVLIGGTLLALLGGAGAAQEPKPKGPPPDDSGTPLPRAGFG
                     RAQNGPPFPDDANSRGRGRAGIGPGGERPKGPVLGNTPFGGRGAPGVGAGPGEFPGLR
                     PGFGGRGGDPDFMRQQDPEMFALVSQDEQLEQQTAELSDQVRRASGEQRDKLKTQLRD
                     LVNKHFEIRQQRRELQLKRMETELARLREAIKERNEVRESIIQQRLTELTGEVKDLGF
                     "
     gene            complement(8216..8788)
                     /locus_tag="VFB80_24885"
     CDS             complement(8216..8788)
                     /locus_tag="VFB80_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37100.1"
                     /translation="MKQEPDLTAEHLRLVTSRALPAAAELEADTAALREEFLQLGRAV
                     EAASADFDEAALVAKLTRECLAHEVQPAVAPAVKQPSSWAPLVLSAALAAAALVAIVR
                     TAILWSAPDFAALQPQSSFDSAANNDVAGTENSDQLRPAWSDPLDEEIASAQSAVEGM
                     ADRATDLDGALDSAGSRLKSIFEELASGSL"
     gene            complement(8797..9369)
                     /locus_tag="VFB80_24890"
     CDS             complement(8797..9369)
                     /locus_tag="VFB80_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN37101.1"
                     /translation="MAAGEDRQSDMQPSVSEGGLRSVSEGGLRVARWVAAHGRAVRGY
                     LLGMVRRPDVADDLLQEVFRRAWQFRDRYRDEGRERAYLLRIADRLVADRGRRLHREI
                     TIDEETWQSIEPALTGTADEALAEMETSIEVATALDKLTPVQRRVLLLRYFGELTFEE
                     IAAALDCPLGTALSHCRRGLLNLRRLMPVR"
     gene            complement(9918..10976)
                     /locus_tag="VFB80_24895"
     CDS             complement(9918..10976)
                     /locus_tag="VFB80_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZN37102.1"
                     /translation="MESAARDFLIKLLETPSPSGYERPIQDVVRAYVSDFAERVSTDL
                     HGNVIAVKNPGAKMRVMLAGHCDQIGMLISHYNDNGFLHAQTIGGWDAQQLIGQRMTI
                     WTAKGPLPAVIARKPIHLLTDEERKQVVKLEDLWLDIGAKTKDEAKELVRIGDPVTLK
                     LGFQEMRNGLANSPAMDNKCGLWVVIEALRRAAQRELKVAVHAVSTVAEEIGLRGAQT
                     SAFGIDPHVGIAVDVTHATDCPTIDKRQQGDVSLGSGPVIVRGPNMNPVVVERLIGLA
                     QKLELPHQLTASGKAQPNDSNALQINRAGVATGLVSIPNRYMHSAVEMISLDDIDRAA
                     NLLAEFVATLTGDEDFTP"
     gene            11342..>11872
                     /locus_tag="VFB80_24900"
     CDS             11342..>11872
                     /locus_tag="VFB80_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZN37103.1"
                     /translation="MTMRSIQIYDTTLRDGTQGEGVSLSLQDKLQITERLDEIGVDFV
                     EGGYPASNTKDAEYFQRARELKLAHAKICAFGMTRRRGMKAADDLGIQALVAAETPIV
                     TIVGKTWDFHVTEVLRVSLQENLDMIAETIEHLASRGRQVFYDAEHFFDGVAANPEYA
                     AETVLAAARAGAKLVIL"
BASE COUNT         1969 a         3886 c         3872 g         2145 t
ORIGIN      
        1 aacgccacct gcgcggtcag ctgcaccggc accggagaac agttcatccg gaatgcagtc
       61 gcctacgacc tttcagcccg cctgaagtac ctgaagcagc cgctggcaga ggcggtgaat
      121 gaggtcctgc acaagacact ccgcccgtcc gacggcgggc taatcgcctt gggcgcggac
      181 gggacgatcg tcatggacta ttcgaccggc gggatggccc gcgccgcggc ggacgccagc
      241 ggacggcgcg aagtgaagat cgggaaatag agttcaaggt tcaaagttca aagttcaaag
      301 ttgtggagaa gcgacgtccg ccgtcctcaa ccttgaacct tgaactttga actttgaact
      361 attcgccgcg caacgtatta aaaatccggt cgccggcgtc gcccaggccg gggacgatga
      421 acttgcggct gttgagctcc gcgtcgatgg cgcagacgaa gatatcggcg gcgggaaatt
      481 gttcccggac ggcgctgacg ccgtgctgcg aggcaatgag cgaaagcacc ttcagtcgct
      541 tgacgcccca ccgctgcaag gccgctaacg ctgccatcgc cgagccgccc gtggcgagca
      601 tcggatcgac gaccagcgcg acttccaccg gttcggtttt cggcagcttg ctgtagtact
      661 ccaccggccg ggcggtcgcc tcatcgcggt agaggcccag gtgccagacc tccgccgacg
      721 gaatcaagtt gaggatcgga tcgaccatcg ccaggccggc gcgcaggatc ggcgccagtc
      781 caatccgttg tttgagggcg ctgccggtca tcggcaccag cggcgtttca agctcgcggg
      841 gcaccaactc caagtcctgc gtggcctcat aagccagcag cacactcagc cgctgcacga
      901 gctgccggaa ctccgccggc ggcgtagtct tgtcacgcag ccgcgtgatg tggtgcttga
      961 cgagcggatg agcgacttcc gttgttggca tgggggccgg tcggtcggtg gtcggttggt
     1021 cggtggtcgg ttagtcggtg gtcggttggt cggtggtcgg ttagtcggtg gtcggttagt
     1081 cggtggtcgg ttagtcggtg gtcggttagt cggtatgtcg gtgggtcgcg gagtcttgga
     1141 tgaagctttc gtagttttgg atcattgcgc ctagcattcg tccgatcgag gccgacatgg
     1201 cggtgagggc cttgtgttcc tcggcgtcga tgtaaccgca ttgatgagca aagtcgagcg
     1261 acgaatcggt ctcgctgttc tcgccgtcgc aatcggtcag cttgcttaag aagtgcgccg
     1321 catatcttcg cttggcccaa gcttctcgaa ggttgaggca gatcgaccgc gacgaccgcc
     1381 ggatctgact ggtcagcgca tagcgctcct cggctgggaa gcgctttgag atctcaaaga
     1441 tacgcatcgc aaactcgttg gcaagctggt aaatctccag ctccttggca gacttcacgc
     1501 gcataaagac ttccaacgac ttgaatgaat cgagcgacaa accactgacc aaccgaccac
     1561 cgatttaccg accgaccgac caccgaccga cctactgcct tcttgcgtcg aaggccggct
     1621 tgccgctttc ctgggcgatc tgttcctggg agtgccaata gcgccagcga gtcttgtcga
     1681 agccaaagct ctcgccggtc agcctggcga gggcgtcgag aacgggctga ttctgcatgt
     1741 ggacgatctg cacctggggg ccttcgttct gcctcatgaa tgtaccgccg tcgctgaagg
     1801 cggtggtcgt ggcgtttggc ccggcgcggc cgggcaggac gcgggtgtgg gtcgtgacca
     1861 gggcgtcgat cagcggcgaa atggtcgaag ggtcgcgaag cctggccagg gcgatcgccc
     1921 cgcggttcac cttgccgttg tcggcgtcct tgagcgccgt cacgaacgga tcggccacgt
     1981 gcggcggctg cagctcggcc agcttgtcga ggcagtaata aagaatctcc tcgtcgaagt
     2041 cggccagcgt gcgatcgacc aggatggcga cggcgtcggg cgtgttgatt ttcgccagga
     2101 tgtcggcgta gagcatcttc acccgccgca ttcgttcctg gccgaaaagc tggcccagcg
     2161 gccgcacggc gaccgggtcg ctaacggctt gcagcgattc gtagccgaac ttcgccttat
     2221 cgggatcgtc caggttcgcc cgccagcggc gaagcttgag cagccagtcc ttctcggcca
     2281 gctcgacgcg ggcccgcgtc tcgaggattt cgatctcctg cggcgtccgc catttgccct
     2341 tgtagaactc gtagccttcc tgccgccgga agtcggagcg cgaaatccat tggcccttga
     2401 ggaactgata gcccatcgcg taccgcgcct gctggtggtt cggatcgatc tcgatcatcc
     2461 gccgcaggtg ggcctcccgc tgacgtgtca ggccgtgctt gcggcaccat tcggccagcg
     2521 accactggcc gtcgagcgta tcgggggcca gtggcacgag ccggcgatat tcgatttccg
     2581 ccggcgactg ccgcaccgct tccttcactt gcgccaggcc aagcgtcagc gtgaggccgt
     2641 cggccgtccg caccaggtac cgcgtcagcg gctgctcggc gtggttgagc cactcgcctt
     2701 cgatctggcc gcccgattca aggcgaaaga catctgcccg cgctggcggc gcggccatgg
     2761 cagccgcgca cgccacagca gcgaagagtg cagcctgtcg tttctgcgcc ggggcgagca
     2821 ttgccaggta caatccttcg tagcggacac gcccgttacc cgcgccccta gcccggccgc
     2881 gcgacggcac acggagtgtg cctactacta taaaacctgc cagttctcgg ctgcaacctt
     2941 ttgctgcaat tgcccatgtc cgcgacgcac gaagagcaca aatcgcaggg cccgccatcg
     3001 gttcgctgcg ccgtgattac cgtcagcgat acccggacgc tggaaacaga tactggcggt
     3061 caggcagtga tcgctcacct taccgccgcg gggcacgtgg tcgtgcagcg cgagatcatt
     3121 cccgacgagc ccgcgcggat gcggcccctg ctcgaaacgc tgtcccgacg cgacgacctc
     3181 gacgccatcc tgctaacggg cggaactggc atcaccagcc gcgaccagac gttcgagacg
     3241 gtcagcggat tgctgacgaa gccgctgccc ggatatggcg agctgttccg catgctcagc
     3301 ttccaggaga tcggcccggc ggcgatcctc agccgggccg tcgggggcct gatcggtcgc
     3361 aagatcgtgc tcacgatgcc cggctcgccc gccgccgtgc gcctggcgat ggagaagatc
     3421 atcgttcccg aattgccgca tctggtgcgc gaggcgcgac ggtgatccat gccgctcagc
     3481 gaaaatccct acgaatcgcc gagaattccc aacgcagtcg ccgctccgca agctccgccg
     3541 cctgggcacc acggctggaa gtttgtcctg ctgggaatcg cgattgcctg cggcatcgtg
     3601 tccttagcgc cgtggctggg tgtgctgttt gccatcttca gcgcgccggt gtttctgcgg
     3661 tatttcgtct ttggctggaa gcggccgcag atgacgccgt gggactatgc cggctcgcgc
     3721 gccgcgggcg ccttcggcgc gatcggtttc gggttgagca tcctcgccgc ggtgagcggc
     3781 gcgttctttg gcacctgcaa tgtcgccggc tggtcggtcg cgttttcgac gctggccacg
     3841 aacatccact acgagcgcgt catcgccgcc gcggtcgcca tcggcgtcat ggcagggctg
     3901 gcagccgccg tgacggccct cgtgtggctc gtgcggttct attaccctcc gcgagcttga
     3961 cgcgccaggc agcaatcgca gcccccactt tcgggcagcg ggcgtcttca cgccgtttta
     4021 gcgcgttctt catgatcggc tgcttgggct gcgctaacct ttaacccgcg tggcctgggg
     4081 gccgcggtcg gaagctgttg cgagggcagg cacttagggc gatgattgaa actcgaaggg
     4141 atcgttcgcc tgccccggag ctgatatgag caaacacctc catcgcgacc tcgagaacct
     4201 gaataccgag ctgctgagca tctcggcgat ggtggaggag atgatcgaca aggcgacgct
     4261 ggccttgagc gagcggcggt acgagctggc cgacgaggtg gtgcagtcgg acgagtacgt
     4321 cgatgagcat gaagttcacg tcgaggaaga gtgcctgaag atgctggccc tgcaccagcc
     4381 ggtggcggtc gatttgcggc ggatcgcgac ggtgctgaag gccaacgccg acctggagcg
     4441 gatcgcggac ctggcggtga gcatcgccca gcgggcgcgg gccatgcacg agttcccggc
     4501 gtttccgatc ccagagccgc tgcggccgat ggtgctgctg gcgacgcaga tggtccgcgg
     4561 ggcgatgaac gccttcgtga acaccgacaa tgccgcggcc cggcggatta tcgccatgga
     4621 caagaccgtc gatcagtaca accgcgagat catcgacgag ctgcagaagc tgatgcagaa
     4681 gcgtccggag gccgtgccgg ccgccgtgaa ctgcttctcg gccgtgcggc acatcgagcg
     4741 gatcgccgac cacgccacga acatcgccga agacgtgatc tacctcgtcg agggagacat
     4801 tgtccggcat cgaatggatc agccgacgtc tgagacgccg aagtagcgcc gaagtagcgc
     4861 agaagtagca acgttccctg gggaaatgga aatggctaag accaaagtgc tcgtgatcga
     4921 agacgaccgc gcgctggcgg aggtgctcac ctacaacctc aagcaggcgg gctacgacgt
     4981 gctgctggcc acggacgggc aggacggcct gctgcaggcc cagatcaaat cgcccgacat
     5041 cgtgctcctc gacctgatgt tgccggtgat cgacggcctc gacgtctgcc gccgcttgcg
     5101 ggccggaacg ggcacgaagg acctgctcat cgtcatggtc acggccaagg ccgaagagag
     5161 cgacgagctg gtcggattct cgctgggggc cgacgactat gtgactaagc cgttcagcgt
     5221 gaaagtgctg ttggagcgga tcaaggccct ccgccgtcgc cggcaaaccg agggagtcac
     5281 cgacgaggtc acgagcaagc tgggcgtgat cgtcgatcgc cgccggcatc aggcgaccgc
     5341 cgagggcaag ccactggagc tgacgcggag cgagttccgc ctgctcgaca cgctcatccg
     5401 ccagcccggc cgcgtgttcc accggtcgga attgatcgat gccgccctgg gcgaggatac
     5461 gatggtcatg gagcgcacca tcgacgtcca catccgtgcc ctccgccgca agctggcgga
     5521 ccgcgccgac gtgatcgaaa ccgtacgcgg cgtcggctac cgcttccgcg acaccgagcc
     5581 gatctgcgag ccggcgtgaa ctgccgccca attggcagcg ctctcgccgc ggactagcat
     5641 cactgtgcgt tctgatcgcc ccttctctct caggtggccc atgaagttgc tgcgctgtgt
     5701 tgtttcggcg gctggcacca ttctctgtat tgcagcagcg tccgcgctcg ccgcagatgt
     5761 cccgtcggtg gtgttcctgt ggcctgcggg cgcggcgacg ctacaagggg ggaacgagaa
     5821 agaaatcacg acccccgccg acgtaaagcc gggcgagcga atcaactcga tccgcaacgt
     5881 tcacaacccg tcgctcgagg tgcatctgcc gccggcggat aaggcggtgg ggactggcgt
     5941 aatcgttgca cccggcggcg gccaccggca attggtgtgg ggctcggaag gaaccgacat
     6001 cgcgcagttt ctgaacgaaa tcggcgtggc ggcgttcatc ctcaagtacc gcctggagca
     6061 gacgcccaat tacaagtaca cggtcgaagg cgaggcattg gccgacacgc agcgggccat
     6121 tcgcatcgtc cgcgcgcgag ccaaggagtg gggcgtgcag cagaatcgga tcggcgtgct
     6181 cggcttctcg gctggcggcg cgctggccgc cctggccgac atccacttcg atcgcggcaa
     6241 ggccgattcc tccgatccga tcgagcagca gagctgccgg cccgatttcg tcggcctggt
     6301 ctatcccggc tggcggccca tggacctcac cgcgccgaag gacgccgcgc cggcgtttct
     6361 caccagcgcg ggtctcgacg acgcctcgca cgcccgagcg acggtcgact ttcaccagtc
     6421 gctgttcaag gcgggcgtgc agaccgaatt gcacatttac gcccgcggtg gtcacggtgg
     6481 ggcgattaag ccccgcgacg gcatcccctt cggcaagtgg cacattcgct ttcaggaatg
     6541 gctcgccgac ctgggtatgc tcaagcaacc ctagtcgcgc ccgcgctcgc cgcgtccgat
     6601 tcgctcatcc cggtcctcac gttcgccgcg cccttcgcgc tcgcgcgatt cttcgcgctc
     6661 gccgcgcggc gggccgcgga attcaccgcc cggaccgccc ggtccgcccg gtcgcgggct
     6721 gcggccgaaa tcgccaccga agccgggccg attggctggt gggccaaagc cggggccaaa
     6781 gccgcccatc cggccgaact ctccggggcc gcgcgtgccg cgtgggccgc cacgtccgcc
     6841 gggtccagcc ggcggaccct gcggacggcc gccgcgggcc gtggctgggc gaccgccggg
     6901 gcgttggttc ttcagttcac gaagctcagc gaggatctga tcgagcttct gttccagccg
     6961 gctgatgcgc tcgtccaggg cgccttcgcc gccgcccctg ccaccaaatc cgccgccgcc
     7021 aaatccgcgg acgccgccta gctggccgcc ctgagcgccg ccgaaaccgc caccgaaacc
     7081 gccacccccg cccacgcctg gtcgtgccat cggcgtgcgg cccggcccac cctcggggcc
     7141 gcgtccttca ggccggcgac cctccggagc gccgcggcct tccggtgcgc gctcttgcgg
     7201 gccacggccg gcatcgcggt cttcgccgcc gcgccgctct tccccgccac cgcgatcgcg
     7261 ctcgccggcg ccttccccgc cgcggcgcgc gccttcggat tcctgggcgc gggcgagcga
     7321 gaggcaacag ccgatcgcga cactgcccat gacaaagccg gctagccgcc ggcacgattg
     7381 cacgttgaac ttcacgagac aagctcctga gagaaggaag agcacttgcc cggcggcttt
     7441 gtgctcgaag aggggaatca tgcgggccag catcgcgccg ccccgcctca agtagtcagt
     7501 tctgcttcat tcaacgccga acgccggcga aagttgcggc gcagcttcaa aatcccaggt
     7561 ccttgacctc gccggtgagc tccgtcagcc gctgctggat gatcgattcg cggacctcgt
     7621 tccgctcctt gatcgcttcg cgcagccggg caagttcggt ctccattcgc ttgagctgca
     7681 gttcgcggcg ctgctggcgt atttcaaaat gtttattgac cagatccctg agttgcgtct
     7741 tgagcttgtc gcgctgttcg ccgctggcgc gacgaacctg gtcggacaac tccgccgtct
     7801 gctgctccag ttgttcgtcc tgactcacga gcgcaaacat ctcggggtcc tgctgccgca
     7861 tgaagtctgg gtcgccgccg cgcccgccga aaccgggacg taatcccgga aactcgccgg
     7921 ggccagcgcc gacgcccggt gcaccgcgac cgccgaaggg agtgttgccg agcacgggcc
     7981 ccttcggtcg ctcaccaccc ggcccaatcc ccgctcttcc gcgcccgcgg gagttggcgt
     8041 cgtcgggaaa ggggggccca ttctgcgcac gcccgaagcc ggcccgcggc agcggcgtac
     8101 cggaatcgtc gggcggcgga cccttgggct tgggttcttg cgccgcgcca gcgccgccga
     8161 gcagcgccag caacgttccg ccgatcaaga ccttccgcca catgggaatt tcctcctaaa
     8221 gggagccgct tgccaattcc tcgaaaatcg acttcagccg ggagccggcg ctgtctaacg
     8281 cgccatccag atcggtcgcg cgatctgcca ttccctcgac ggcgctctgg gccgacgcga
     8341 tttcctcgtc gagcggatcg gaccaggcag ggcgaagctg gtcgctattc tctgtcccgg
     8401 cgacatcgtt gttcgcggca gagtcgaacg aactctgcgg ctgaagcgcg gcgaagtcgg
     8461 gggcggacca caagatcgcc gtgcgaacta tcgccaccaa agccgctgca gccagcgcag
     8521 cgctcagcac gagcggcgcc cagctgctcg gctgtttcac cgctggcgcg accgccggct
     8581 gaacctcgtg ggccagacac tcgcgcgtca gcttcgcgac cagcgccgct tcgtcgaagt
     8641 cggcgctcgc tgcttcaacg gcgcggccga gctgcagaaa ctcctcgcgc agggcggctg
     8701 tgtccgcctc cagctcggcc gccgcaggca gcgctctcga cgtaacgagt cgcaaatgct
     8761 cggcggtcag atccggttct tgcttcatgt taccgcctat cgcacgggca tcagcctgcg
     8821 caaattcagt aatcctcgcc ggcagtggct gagcgcggtt cccagcgggc agtccagcgc
     8881 cgcggcgatc tcctcgaacg tcagttctcc gaagtatcga agcagcagca cgcgccgctg
     8941 gaccggcgtc agcttgtcca gggcggttgc cacctcaatg ctggtttcca tctccgccag
     9001 cgcctcatcc gcagtgccgg tgagcgccgg ttcgatggac tgccaggttt cttcatcgat
     9061 cgtgatctcg cgatgcagcc gccggccgcg gtcggcgacc agccgatcgg cgattcgcag
     9121 caggtaggcc cgctcgcggc cttcgtcgcg gtagcggtcg cggaactgcc aggcacgccg
     9181 aaacacctcc tgcagcaggt cgtcggccac gtctggccgg cggaccatgc ccagcaaata
     9241 tccgcgcacg gcccggccat gcgcggcgac ccaccgcgca acgcgcagcc cgccttcgga
     9301 aacactgcgc agtccacctt cggaaacact gggctgcatg tcggattgcc gatcctcgcc
     9361 cgcggccatg ctgcattcct caacgttcca gccgcctggg ttattgcagc cccttgcgtc
     9421 cgattccgga cggcagaatc actttgatca tgagcgtatc catttcgcgg cgcatcagcc
     9481 gccgtctggg aagcggagcc gccgccggac cttggccaga actcttgtcg cagcaatgag
     9541 ttacgctatg gccccggcgc tttggcggct gctggcatat ccgctgcatt tcagtttaag
     9601 tggcctggaa atgaaacgcc ggagcgaccc ggatgattcg cgaattgttg gcggtggcgc
     9661 ggtggatgtt cgcggaagtc gtgatgatcg gcatcgccct gtggaccctg ctctggttcg
     9721 ccggggcgct gcgctgttga tcgaaccacg gaattgatcg gaacgaagca gacgcccggg
     9781 cggtcagccc attgccagct cactggctgc ttggtgcgct ctgcttcgtt ccgatttttt
     9841 cgtagcccaa gcgtccgcgc tggtgcgggc catcttcgcc agctgtgagt aagcaagcgg
     9901 ggacgcttgc tctacgatca gggcgtgaag tcctcgtcgc ccgtgagcgt cgcgacgaat
     9961 tcggccagca ggttggcggc gcggtcgatg tcgtcgagcg agatcatctc gacggcgctg
    10021 tgcatgtagc ggttgggaat gctgaccagg ccggtggcca cgccggcccg gttgatctgc
    10081 agggcgttgc tgtcgttcgg ctgggccttg ccgctggcgg tgagctggtg gggcagctcc
    10141 agcttctggg ccaggccgat cagccgttcg acgacgaccg ggttcatatt cggcccgcgg
    10201 acaatgacgg ggccgctgcc gagactgacg tctccctgct gccgcttgtc gatggtcggg
    10261 caatcggtgg cgtgggtgac atcgacggcg atgccgacgt gcgggtcgat gccgaacgcg
    10321 ctagtctgcg ccccgcggag cccaatttct tcggcgacgg tcgagacggc gtgcacggcg
    10381 accttcagct cgcgctgggc cgcccgccgc agggcctcga tcacgaccca caggccgcac
    10441 ttgttgtcca tcgccggcga gttcgccagg ccgttacgca tctcctgaaa gccgagcttc
    10501 agagtcaccg ggtcgccgat ccgcaccagc tctttggcct cgtccttggt cttcgcaccg
    10561 atgtcgagcc agaggtcttc gagcttcacg acctgcttcc gctcttcgtc ggtcagcagg
    10621 tggatcggct tgcgcgcgat caccgccggc agcgggccct tggcggtcca gatcgtcatt
    10681 cgctggccga tgagctgctg ggcgtcccag ccgccgatcg tctgggcgtg caaaaagccg
    10741 ttgtcgttgt agtgcgagat cagcatgccg atctggtcgc agtggccggc aagcatgacc
    10801 cgcattttcg ccccgggatt tttcaccgcg atcacgttgc cgtgcaggtc ggtcgagacc
    10861 cgctcggcaa agtcgctgac gtacgcgcga accacgtcct ggatcggccg ctcatagccg
    10921 gaagggctgg gggtctcgag cagcttgatg agaaagtcgc gggcggcgga ttccatttcg
    10981 ggagtcgggg gtcaggggtc gagagtcggc aatcagggac agtattgccg gggccgtttg
    11041 tgccgcaaag agcggtcgcg ctggcgttga cagcagctgg gggaagacgt agcgttatgt
    11101 cgaataccct tacggcttgg gccatgaccg ttgcttccta taacgcgatt tccccgccgc
    11161 tggctcgtgc cggcgtcgtc ggcgtcgtga ttattattgg gagccttcgg gtctaagggc
    11221 caagcggtaa cacaccgaac tttgcaagcc ctggaccccg gcgggttcag ggctttttgt
    11281 tttggtcatt tgtcattgga agggcagatg ccaacgacaa gtgacaagtg acaagtgaca
    11341 aatgaccatg cgcagtatcc aaatctacga caccactctc cgcgacggca cgcagggcga
    11401 aggcgtgagc ctctcgctcc aggacaagct gcagatcacc gagcggctgg acgagatcgg
    11461 cgtcgatttc gtcgagggag gctatccggc cagcaacacc aaggacgccg agtatttcca
    11521 gcgggcgcgg gagctgaagc tggctcacgc gaagatttgc gcctttggca tgacccgccg
    11581 ccgcggcatg aaggcggccg acgatctggg cattcaggcg ctcgtcgcag ccgagacgcc
    11641 gatcgtgacg atcgttggca agacctggga ctttcacgtc accgaagtgc tgcgcgtgag
    11701 cttgcaggaa aacctcgaca tgatcgctga gacgatcgag cacctggcca gccgcggccg
    11761 gcaggtgttc tatgacgccg agcacttttt cgacggcgtt gcggccaacc cggagtacgc
    11821 ggccgagacg gtcctcgccg cggcccgagc cggggcgaaa cttgtcatcc tc
//
LOCUS       DASRJB010000939         6661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2251278, whole genome shotgun sequence.
ACCESSION   DASRJB010000939 DASRJB010000000
VERSION     DASRJB010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251278"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFB80_24905"
     CDS             <1..718
                     /locus_tag="VFB80_24905"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN37104.1"
                     /translation="ILFEQPPKLKVELQKQLGRGFRAIKIGWRPFGRVSRQLDELLIK
                     TARDTVGPEVELMVDAGGSEQFWPHNVGWARATARMLGDYGVTWFEEALKPDDFDGFA
                     ELKVGSPVLIATGEVLTRRQSFQPLIDGRAVDIIQPDLTKCGGLSEGRKLGWSAYDHG
                     VLLVTHGWNTAIGVAADLALAAALPVARWVEYQTGVPYIEEIIDPPFALDAEGLLRIP
                     TGPGLGVTLNRDAIARFSPK"
     gene            complement(722..2155)
                     /locus_tag="VFB80_24910"
     CDS             complement(722..2155)
                     /locus_tag="VFB80_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN37105.1"
                     /translation="MNCQAHLHRGLSPTSLARRWFLRDCGIGLGASALAELAGKASAA
                     PLPADPLAAKAPHFAAKAKSVIYLFMAGAPSHLEMFDNKPELAKWDGKLPPAELLKGY
                     RAAFINPNSKLLGPKFKFARHGNCGAELSELLPHLATVVDEIAIVKSMATDAFNHAPA
                     QIQMNTGSQQFGRPSIGAWVTYGLGCETQELPAFVVFSSGKKGPSGGNSNWGSGFLPT
                     VYQGVQFRGGSEPVLFLNSPRGVDQSLQADSIDAIRTLNQHRLAQVGDPEIATRTAAF
                     EMAFRMQTSAPDLMDLSQEDKETLELYGAEPGKSSFANNCLLARRLVERGVRYVQLFH
                     EAWDQHGNLVKDIKQNCLDTDRACTALIKDLKQRGLLDETLVVWGGEFGRTPMVQGGT
                     DGRDHHPNAFTMWLAGGGVKSGLTLGESDEFGFNAIQDRVHVRDLHATLLHLLGLDHQ
                     RLTFKFQGLDAKLTGVEPARVVKELLT"
     gene            complement(2160..5276)
                     /locus_tag="VFB80_24915"
     CDS             complement(2160..5276)
                     /locus_tag="VFB80_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN37106.1"
                     /translation="MKLSLLVAVLSCAVTAPAADRVDFQREVRPLLADKCFACHGRDA
                     EHRQSGLRLDERPAALQGGDSGEPAIVPGQAEKSELVRRVFSVDADERMPPPDSKKSL
                     TAAEKDLLRRWVAEGAEYKAHWAFTAPVRPPVPAVKNVAWPKNDIDRFILARLEKEGL
                     APSPPADAATLLRRLSLDITGLPPSVAPSLLPSVALSGSQDGGTERQRDGVKEIVQRL
                     LASPHYGERWGRIWLDGARYADSDGYEKDKPRFVWAYRDWVVGALNRDLPYNQFVIEQ
                     IAGDLLPNATQEQIVATGFLRNSMINEEGGVDPEQFRMEAMFDRMDAIGKSILGLTVQ
                     CCQCHNHKYDPFTQEDYYRMFAFLNDTHEANVAVYPPDQLQMRADLLRQIGEVEADLQ
                     HRTPDWQERMAAWEESVRGDQPQWTVVKPEQDVSGGQKHYVLADGSILAQGYAPTKHT
                     TEFEVTTDVANITAVRLELLNDPNLPLGGPGRSIYGTCALTEFRLEAGPADGSAKLAE
                     VKFTGGAADVNPPEKELEAIYGDKTNKRRVTGPIEYALDRKDETAWGIDLGPGRRNVP
                     RKAVFNLEQPLKLEGGAKLKFRLTQNHGGWNSDDNQNHNLGRFRFSVTAAEGAKADPL
                     PAPVRRAFETPRGERTPAQVAAIFSYWRTTVPDWKEANDKIEALWQQHPPGTSQYTLA
                     ARDEHRPTFLLARGDFLKPTRPVAAGAPAFLHALDRESPTRLDFAHWLVDERSETTAR
                     AIVNRVWQGYFGTGLVSTSEDFGLQGEAPSHPELLDWLAVEFMQQGWRLKWLHELIVS
                     SATYQQSSRVTRELLARDPHNRLLARGPRFRLDAEGVRDVALAASGLLNAKLGGPPVY
                     PPAPDFLFVPPASYGPKVWTEAKGDDRYRRALYTFRFRSVPYPVLQTFDAPNGEFACV
                     RRVRSNTPLQALATLNEPLFLECARALALKTVTEGGANDAERVEFAFRRCTSRTPDKK
                     ESAALLALLTNQIERFSAADAKPWELAANDLANPPALPAGTTAAQLAAWTAVSRVLLN
                     LDETITKE"
     gene            5439..6236
                     /locus_tag="VFB80_24920"
     CDS             5439..6236
                     /locus_tag="VFB80_24920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN37107.1"
                     /translation="MDSLFSVADQIILVSGGTRGIGFAIAAGFAQRDARVILTGRTQS
                     AAEESASKLAAGAKHAPRGLACDVTKTAHIKELACRVLAECGQVDALVNVAGVNRRKP
                     SLAVSEDDYDFILNTNLKGAFFLAQEIGRSMCERGRGCQINVASLNTDRPLKNVLPYA
                     MSKAAIAHMTRGMAVEWGPRGVRVNGIAPGFILTDLTRKLWSDPTMHDWGLANTPLRR
                     LGLPDDIVGAAIFLASPAAAFITGQTLYIDGGFTAGWAWPIPESGGQ"
     gene            6353..>6661
                     /locus_tag="VFB80_24925"
     CDS             6353..>6661
                     /locus_tag="VFB80_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37108.1"
                     /translation="MLVRLSLALLAVVNFASMADGEPPVVELLKLHTSEAEAYRMCRD
                     AAHQESLELNPKPVFNWTNLVGEHTQYGHLFVWLHRGRPEVIGTIFSTRMSDAAKRKV
                     V"
BASE COUNT         1055 a         2194 c         2216 g         1196 t
ORIGIN      
        1 gatcctgttc gagcagccgc cgaagctcaa ggtcgagctg caaaagcagc tcggccgcgg
       61 ctttcgggcg atcaagatag gctggcggcc gtttgggcgc gtcagccggc aactcgacga
      121 gctcctcatc aagacggccc gcgacacggt cggccccgag gtcgagctga tggtcgatgc
      181 cggcggcagc gagcagttct ggccgcacaa cgtcggctgg gcccgcgcca cggcccggat
      241 gctcggcgac tacggcgtga cctggttcga ggaagccctg aagcccgacg atttcgacgg
      301 cttcgcggag ctgaaggtgg gctcgcccgt gctgattgcg accggcgagg tgctcacgcg
      361 gcgtcagtcg tttcagccgc tgatcgacgg ccgggccgtc gatatcattc agccggacct
      421 gaccaagtgc ggcggccttt cggaaggccg caagctcggc tggtcggctt acgaccacgg
      481 cgtgctcttg gttacgcacg gctggaacac ggcgatcggc gtcgcggcgg acctggcgct
      541 ggctgcggcc ctgccggtcg cccgctgggt cgaataccag accggcgtgc cctacatcga
      601 ggaaattatc gacccgccct tcgcgctcga cgcggagggc ctgctgcgga ttccaaccgg
      661 tccgggcctc ggcgtcacgc tcaaccgcga cgccatcgcg cggttctcgc cgaagtagca
      721 gctacgtcag cagctctttg acgactcgcg ccggttcgac gccggtgagc ttggcgtcga
      781 ggccttggaa cttgaaggtc agtcgctggt ggtccaggcc gaggaggtgc agcagcgtcg
      841 cgtgcaggtc gcggacatgg acgcggtctt ggatagcgtt gaagccgaat tcatcgctct
      901 cgcccagggt caagccagat ttcacaccgc cgcctgcgag ccacatcgtg aaggcgttgg
      961 ggtgatggtc gcggccgtcg gtgcccccct ggaccatcgg cgtgcggccg aactcgccgc
     1021 cccagacgac gagcgtctcg tccaacaggc cccgctgctt gaggtccttg atcagggcgg
     1081 tgcaggcgcg gtcggtgtcg aggcagttct gcttgatgtc cttgacgagg ttgccgtgct
     1141 ggtcccaggc ctcgtgaaag agctgcacgt accgcacgcc ccgctcgacc agccggcggg
     1201 ccagcaggca gttattggcg aacgacgact tgcccggctc tgcgccgtac agctccagcg
     1261 tttccttatc ctcctgcgag aggtccatca ggtctggggc gctggtctgc atgcggaagg
     1321 ccatctcgaa tgccgcggtg cgggtggcga tctccgggtc gccgacctgc gccaggcggt
     1381 gctggttgag cgtgcggatg gcgtcgatgg agtcggcctg cagcgactgg tccacgcccc
     1441 gggggctgtt caggaaaagc accggctcgc tgccgccacg aaactgcacg ccctgataga
     1501 cggtcggcag aaagccgctg ccccaattgc tgttgccccc gctggggccc tttttgccgc
     1561 tgctgaagac cacgaacgct ggcagctcct gcgtctcgca gcccaggccg taggtgaccc
     1621 aggccccgat gctcggccgg ccgaactgct ggctgccggt gttcatctgg atctgggccg
     1681 gcgcgtggtt gaaggcgtcc gtcgccatcg acttcacgat cgcgatctca tcgaccacgg
     1741 tcgccaggtg cggcagcagc tccgacagct ccgccccaca gttgccgtgc ctggcgaact
     1801 tgaacttcgg acccagcagc ttgctgttgg gattgatgaa cgccgcccga tagcccttga
     1861 gcaattccgc cggcggcagc ttgccgtccc acttggccag ctccggctta ttgtcgaaca
     1921 tctccaggtg gctcggcgct ccggccatga agagatagat cacgctcttg gccttggcgg
     1981 caaagtgcgg ggccttcgcc gcgagcggat cagccggcag cggcgcggcg ctggcctttc
     2041 cggccagttc tgcaagcgct gacgcgccca ggccgatgcc gcaatcgcgc aggaaccagc
     2101 ggcgggcgag cgatgtcgga gagaggcctc gatggaggtg ggcctggcaa ttcatgggtt
     2161 cattccttcg tgatcgtctc atccagattc agcagcacgc ggctgactgc ggtccacgcg
     2221 gcaagctgcg ccgctgtcgt gccggcgggc aatgccggcg ggttggccag gtcgtttgcg
     2281 gctagctccc agggcttcgc gtcagcggcg ctgaagcgtt cgatttgatt tgtcagcagc
     2341 gcgagcagcg cggccgactc tttcttatcc ggcgttcgcg acgtgcagcg gcggaaggcg
     2401 aactccacgc gctcggcgtc gttcgcgccg ccttcggtca cggttttgag cgcgagggcg
     2461 cgggcgcact ccaggaacag cggctcgttg agcgtggcca gcgcttgcag cggcgtgttg
     2521 ctgcggacgc ggcggacgca ggcaaattcg ccgttgggag cgtcaaaggt ctgcagcacg
     2581 ggatagggca cgctgcgaaa gcggaaggtg tagagcgcgc ggcgataacg gtcgtcgccc
     2641 ttcgcctcgg tccagacttt cggcccatag ctggccggcg gaacgaacag gaaatctggc
     2701 gcgggtggat agaccggcgg accgccgagc ttcgcattga ggaggccgct tgccgcgagg
     2761 gccacgtcgc ggactccttc ggcatcgagg cggaatcgcg gcccgcgagc gagcaggcgg
     2821 ttatgcggat cgcgggccag caattcccgc gtaacacgcg acgactgctg ataggtggcc
     2881 gacgagacga tcagctcgtg cagccatttg agccgccagc cctgctgcat gaactcgacg
     2941 gccagccagt cgagcagttc cgggtgcgaa ggggcttcgc cttgcaaccc aaagtcctcg
     3001 ctggtgctga ccagccccgt gccgaagtag ccctgccaga cgcgattcac aatggcccgc
     3061 gccgtcgtct ccgagcgctc atcgacgagc cagtgggcga agtcgaggcg cgtagggctc
     3121 tctcgatcga gcgcgtgcag aaacgccgga gcaccggccg cgacgggccg cgtcggcttg
     3181 aggaagtcgc cgcgagcgag caggaacgtt ggccggtgct cgtcgcgggc ggccagggtg
     3241 tattgcgacg tccccggcgg atgctgctgc cagagcgctt caatcttgtc gttcgcttct
     3301 ttccaatccg ggactgtcgt ccgccagtag ctgaagattg cggcaacctg tgccggcgtc
     3361 cgctcgccgc gcggcgtctc gaaggctcgc cgcacaggcg ccggcagcgg atcggccttt
     3421 gcgccttccg ccgccgtcac gctgaaccgg aagcggccga ggttgtgatt ctggttgtcg
     3481 tcgctgttcc agccgccgtg gttctgcgtc aggcggaact tgagtttcgc gccgccctcc
     3541 aacttcagcg gctgctcgag gttgaagacc gcctttcgtg gcacattgcg gcggcccggg
     3601 ccgaggtcga tgccccaagc cgtctcgtcc ttgcggtcga gggcgtactc gatcggcccg
     3661 gtcacgcggc gcttgttggt cttgtcgccg tagatcgcct cgagctcctt ctcgggcgga
     3721 ttcacgtcgg cggcgccgcc ggtgaacttc acctcggcca acttcgccga gccgtcagcc
     3781 gggccagcct ccaggcggaa ctcagtcagg gcacaggtgc cataaatcga gcgacccggt
     3841 ccgccgagcg gcaggttcgg gtcattaagc aactccagcc gcacggccgt gatgttcgcg
     3901 acatccgtcg tcacctcaaa ttcggtcgtg tgcttcgttg gggcgtagcc ttgagccagg
     3961 atcgagccgt cggccaggac atagtgcttc tggccgccgc tgacgtcctg ctccggcttg
     4021 acgacggtcc actgcggctg atcgccgcgc accgactctt cccaggcggc catccgttct
     4081 tgccaatcgg gagtgcggtg ctggaggtcg gcctcgacct cgccgatctg ccgcagcagg
     4141 tcggctcgca tctgcagttg gtccggcgga tagacggcga cgttcgcctc atgcgtgtcg
     4201 ttcaggaagg cgaacatccg gtaatagtct tcctgcgtga acggatcgta cttgtggttg
     4261 tggcactggc agcactgcac ggtcaggccg aggatgctct tgccgatcgc gtccatccgg
     4321 tcgaacatcg cctccatccg gaactgctcg ggatcgacgc cgccttcctc gttgatcatc
     4381 gagttccgca gaaagccggt ggcgacgatc tgctcctgcg tcgcgttggg caataggtcg
     4441 ccggcgattt gctcgattac gaactggttg tagggcaggt cgcgattgag cgccccgacc
     4501 acccaatcgc gataggccca cacaaaccgc ggcttgtcct tctcatagcc atccgagtcg
     4561 gcataccgcg ccccatccag ccagatccgg ccccagcgtt cgccgtagtg gggtgaggcg
     4621 agcaggcgct ggacaatctc cttcactccg tccctctgtc gctccgtccc tccgtcctgg
     4681 ctgccggaga gagcgacgga gggaaggaga gacggagcga cggagggcgg cagtcccgta
     4741 atgtcgagcg acagtcggcg gagcagcgtg gcggcgtctg ccggcggcga gggggcgagc
     4801 ccttccttct ccaaccgggc gagaatgaac cggtcgatgt cgttcttggg ccaggcaacg
     4861 ttcttgacgg ccggcacagg cgggcgcacg ggcgcggtga acgcccagtg ggccttgtac
     4921 tctgcgccct cggccaccca gcggcggagc aggtccttct ccgcagccgt cagcgatttc
     4981 ttcgaatccg gcggcggcat ccgttcgtcg gcgtccaccg aaaagacccg gcggaccagt
     5041 tcgctcttct cggcctgccc gggcacgatc gccggctcgc cgctgtcgcc gccttgaagc
     5101 gccgcggggc gctcgtccag tcgcagcccg ctctggcgat gctcggcatc gcggccgtgg
     5161 caggcaaagc acttgtcggc gagcagcggc cggacctcgc gctgaaaatc gaccctgtcg
     5221 gcggccggcg cagtaaccgc acaagacagt acggctacaa gcagagagag tttcatagca
     5281 ggcttcagca gaaggctcag gcgggctcta tgttagtgtc atttctcccg ccgcaggctg
     5341 caaccgtacg tcccgcctgc agggcggaat tcaaccgccg acgatccaat aacattttga
     5401 ggccccgccc accgggcgga tcctatcaag gtgccagcat ggactccctg ttctccgtcg
     5461 ctgaccagat catcctcgtc tccggcggta cgcgcggcat tggctttgcc attgctgcgg
     5521 gctttgctca gcgggatgct cgcgtgatcc tcacgggccg gacgcaatca gccgccgagg
     5581 agtcggcgtc aaaactggcg gccggcgcga agcatgcccc gcgggggctg gcctgcgatg
     5641 tgactaagac cgcgcacata aaggaattgg cctgccgcgt gctcgcggag tgcggccagg
     5701 tcgatgcgct cgtcaacgtg gccggcgtca atcgccgcaa gccgtcgctc gccgtcagcg
     5761 aagacgacta tgacttcatc ctcaatacca atctcaaagg cgcgttcttt ctggcccagg
     5821 aaattggccg ctcgatgtgc gagcgcggcc gcggctgcca gatcaacgtc gcttcgctca
     5881 acaccgaccg gccgctcaag aatgtgctgc cctatgcgat gagcaaggca gccatcgccc
     5941 acatgacccg cgggatggcc gtcgaatggg gaccccgcgg cgtgcgagtc aacggcatcg
     6001 cccccggctt catcctcacc gacctgacca ggaaactctg gtccgacccg acgatgcacg
     6061 actggggatt ggccaacacg ccgctgcgcc gcctgggcct gcccgacgac atcgtcggtg
     6121 ccgcgatctt cctcgcctcc cccgccgctg ctttcatcac gggccaaaca ctctacatcg
     6181 acggcggctt caccgccggc tgggcctggc cgatcccgga gtcgggcgga cagtgagctc
     6241 ctgttctttg tgtccaggca gaatggaccg cgtggtaacc agccggttgt gactgccgga
     6301 ggatacagac acaatgtctg catctcggcc cggccgtttc aggagtcccg ccatgctcgt
     6361 tcgactctcg ctggcgttgt tggcagtcgt caactttgca tccatggccg acggcgagcc
     6421 gccggtggtg gagttgctca aactccacac cagtgaagcc gaggcgtatc gaatgtgtcg
     6481 cgacgccgcg caccaggagt ctctggagct gaaccccaag ccggtcttca actggacgaa
     6541 ccttgtcggc gagcacacgc aatacggcca tttgttcgtc tggctgcatc ggggccggcc
     6601 ggaggtgatc ggtacgatct tctcgacccg catgtccgac gctgcgaagc ggaaggtggt
     6661 g
//
LOCUS       DASRJB010000940         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2538373, whole genome shotgun sequence.
ACCESSION   DASRJB010000940 DASRJB010000000
VERSION     DASRJB010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538373"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFB80_24930"
     CDS             complement(<1..429)
                     /locus_tag="VFB80_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZN37109.1"
                     /translation="MKSEMLINVSQPEECRIAIVEEGVLEELYIERASQESFVGNIYK
                     GRIVNLEPSIQAAFVDFGVGRNGFLHISDVEPQYFRQGGFDPGESMGPGRMADIDVGD
                     DDEINGEDEPGNNGGEGGEQGPPRQGDRGDRGGRGGRFRRA"
     gene            complement(814..1086)
                     /locus_tag="VFB80_24935"
     CDS             complement(814..1086)
                     /locus_tag="VFB80_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZN37110.1"
                     /translation="MTVVNPQGFHARPAHLFMKLAMTFQAQVEIVKGNEIVNGKSILD
                     LLTLGAGNGTTLTLRASGPDAQAAVDALAQLIEGGFGEAAPADQSS"
     gene            complement(1215..1691)
                     /locus_tag="VFB80_24940"
     CDS             complement(1215..1691)
                     /locus_tag="VFB80_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZN37111.1"
                     /translation="MKFADFVNVASIRADLAADEKPAAIRELVEGLASAGAIAAADVA
                     GIVAAIMKREELGSTGIGRGVAVPHTKHPSVEKLVGTVGVSQEGVDFNSLDGDKVQLF
                     FLLVSPPDRPGDHLRALENISRQLRDDTFCRFLKQARTAHDIQLLLEEADTNQFAS"
     gene            complement(1755..2102)
                     /gene="raiA"
                     /locus_tag="VFB80_24945"
     CDS             complement(1755..2102)
                     /gene="raiA"
                     /locus_tag="VFB80_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZN37112.1"
                     /translation="MQTTIAARHGHLASETQERIKTKVEVVRKFLDRITAIGVTVDLE
                     HRERPIVELRVRAEHHDEFVAVEEADNVFAAVDGVIEKMEGQLRRFKERLKEHRATGH
                     KHIEPPPPPTAEA"
     gene            complement(2305..3441)
                     /locus_tag="VFB80_24950"
     CDS             complement(2305..3441)
                     /locus_tag="VFB80_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN37113.1"
                     /translation="MSKLRCVPGGRLRLAVIGVGHLGKIHARLTRQLDGVELVGVVDP
                     SPEARAAAQAELGVAAHADNQPLLGQIDAAIIATPTRLHHAVAHDLLVHGVHVFVEKP
                     VTLNVADADDLIQAAEDRGLVLQVGHVERFNPALVAALPHLAEPKYIDANRCGPYSCR
                     STDVGVVLDLMIHDIDVALSLVEGELAGVSALGAAVIGPNEDWAQARLTFAGGCVVNL
                     SASRVAWQAARTMQVVCCDSIAAIDFGTRKSQVMRPTDAVLSGELDVQSLGPAERAHL
                     KDHLFTEYLPLAELPVGEANALVDEQREFVGAIRGETAVRVNGRDGRRALDVAERILA
                     EIAAHRWDGTAVGPIGPRFDTRESVLRRPHWQQSRRAVVRRKAG"
     gene            complement(3438..>3647)
                     /locus_tag="VFB80_24955"
     CDS             complement(3438..>3647)
                     /locus_tag="VFB80_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZN37114.1"
                     /translation="RNDFSADNIRALAEAHRLIYRAKVGLDHARELLRGADMLVPPVN
                     HLLSFLQNQHEGRNGRGRDRRRAAA"
BASE COUNT          580 a         1227 c         1205 g          635 t
ORIGIN      
        1 cgcgcggcga aagcgtccgc cgcgacctcc gcggtcgccg cgatcgccct gccgcggcgg
       61 gccctgctcg cccccctctc caccgttatt tcccggctcg tcttcgccgt tgatttcgtc
      121 gtcgtcgccg acgtcgatgt cggccatccg gccggggccc atcgattcgc cgggatcgaa
      181 gccgccctgc cggaaatact gcggctcgac gtcgctgatg tgcagaaagc cgttgcggcc
      241 gacgccgaaa tcgacgaaag ccgcctgaat gctcggctcc aggttgacga tccggccctt
      301 gtagatgttc ccgacaaacg attcctggct ggcccgttcg atgtacagct cttccagcac
      361 gccctcttca actatggcga tccggcattc ctcgggctgc gagacattga tcagcatttc
      421 gcttttcatc gcggacagtg ctttcttgtg cgttaaggtg gagccgcggc tgggcggacc
      481 ggcggtatgg cgccgggggc cgagtggacg cgcgaggcgg tcgcccggca tttagcccgc
      541 ggagctgccg acgcatgcgc cagcggccgc gctcctggcg gcgggcgagc ggtcggattc
      601 ccaagcggac gccgggcgaa agcgccggcg cggaagtggc cagccagtcg cggcggaggg
      661 gtaaaaagtc gaacgaaatt cggcaatcgc tcaccgggca attcccggcg ggcgaaggta
      721 gcgcaagatt gcaggcggcg ggcagcagca aatcaggagg cagagaggcc agtggcagca
      781 ttggcgattg tggggccgat agcagcggcg gaactagctc gactggtccg cgggagctgc
      841 ctcgccgaag cctccttcga tcaattgggc cagggcgtcg acggccgcct gggcgtccgg
      901 tccgctggcc cgcaaagtga gtgtcgtacc gtttccggcc cccagcgtca acaagtccag
      961 gatgcttttg ccgttgacga tctcgtttcc cttgacgatt tcgacctggg cctgaaaggt
     1021 catcgccagt ttcatgaaca ggtgcgcagg ccgggcgtgg aatccttgcg gattgacgac
     1081 ggtcaccggc ctgatgcagg gcgaatccaa cgcaagtcct gaatgaatac gcgaaaggta
     1141 gccgaaaacg acacagttgg gacggctgct ggatcccgaa ttctggcgaa ttcggctcct
     1201 tgagactccg ccgcctacga cgcgaactgg ttggtgtcgg cctcttccag cagcagctgg
     1261 atgtcgtggg ccgtgcgggc ctgcttcagg aagcggcaga acgtgtcgtc gcggagctgc
     1321 cgggagatgt tctccagcgc ccgcaggtgg tcgccgggcc ggtcgggcgg cgaaacgagc
     1381 aggaagaaga gctgcacctt gtcgccgtcc aggctgttga aatcgacgcc ttcctggctg
     1441 acgcccacgg tgccgacgag cttttcgacg ctgggatgct tggtgtgggg caccgcgacg
     1501 ccgcggccga tccccgtgct gcccagctcc tcgcgcttca tgatcgccgc cacgattccc
     1561 gccacgtctg ccgccgcgat agcccccgcg gaggccagcc cctcgacgag ctcgcggatc
     1621 gccgccggtt tttcatccgc cgccagatcc gcccgaatcg aagccacgtt cacgaagtcc
     1681 gcaaatttca tcgcttgttc ctttatggtg cgctgcctgc ggcagcgaca tgactgcatt
     1741 gcccgcggca gcggctaagc ctcggcggtc gggggaggcg gcggctcgat gtgcttgtgc
     1801 cccgtcgcgc ggtgctcctt cagccgctct ttgaatcgcc gcaattggcc ttccattttc
     1861 tcgatcacgc cgtccactgc cgcaaataca ttatcggcct cttccacggc cacaaactcg
     1921 tcgtgatgct cggcccgcac ccgcaactcc acgatgggcc gctcccgatg ctccagatcg
     1981 accgtcacgc caatcgccgt aatccggtcc aggaactttc gcaccacttc gactttcgtt
     2041 ttgatcctct cctgcgtctc cgaagccagg tgaccgtggc gggccgcaat ggtggtctgc
     2101 accgcgtgac tccccaggag gtgctgctcg ggatgccgtc cggcggcggg gcgctccccg
     2161 gctcgcaaac tctgcggaca cgttcaagtc tagctgattt accaccgccg gaaagggcct
     2221 gcggtgcgcg gcctcgggcg ggcaaagcta gcgtctggcg cgacgcaaac gcctggccgg
     2281 caagagttta tagagagtga acggctaacc ggctttgcgg cgaacgacgg cccgccggct
     2341 ctgctgccag tgcggtctgc ggagcacgct ctcgcgggta tcgaagcgcg gcccgatcgg
     2401 gccgacggcc gtgccgtccc agcgatgggc ggcgatctcg gccaggattc gctcggcgac
     2461 atccagcgcc cggcggccgt cgcggccgtt cacccgcacc gcggtctcgc cgcggatcgc
     2521 ccccacgaat tcacgctgct cgtctacgag agcattggcc tcgccgacgg gcaattcggc
     2581 cagcggcaga tattcggtga acaggtggtc cttgaggtgc gcccgctcgg ccggcccgag
     2641 cgactgcacg tcgagctcgc cgctcaagac ggcgtcggtg ggccgcatga cctgcgactt
     2701 ccgcgtgccg aagtcgatcg ccgcgatcga gtcgcagcag accacctgca tcgtccgtgc
     2761 cgcctgccag gcgactcgcg aggccgagag gttcacgacg cagccgccgg caaacgtcag
     2821 ccgggcctgc gcccagtctt cgttcggccc aatgaccgcc gcgcccagcg cgctgacgcc
     2881 ggccagctcg ccttcgacca gcgagagcgc cacgtcgatg tcgtgaatca tcaggtcgag
     2941 cacgacgccg acgtcagtcg agcggcagct atacggtccg cagcgattcg cgtcgatgta
     3001 cttgggttcc gccaggtgcg gcagcgcggc gacgagagcc gggttgaacc gttcgacgtg
     3061 gccaacctgc agcaccaggc cgcggtcctc tgccgcctgg atcagatcgt cggcgtcggc
     3121 gacgttcagc gtcaccggct tttcgacgaa cacgtggacg ccgtgtacta gcaggtcgtg
     3181 cgccacggca tggtgcagcc gcgtgggcgt ggcgatgatc gccgcgtcga tctgccccaa
     3241 taatggctga ttatcggcat gggcagcgac tcccagttct gcctgagccg cggctcgagc
     3301 ctcgggggac ggatcgacga cgccgaccaa ctccactcca tccagctgcc gcgtgaggcg
     3361 ggcgtgaatc ttgcccaagt ggccgacgcc gatcactgcc aggcggagcc tgccgccggg
     3421 aacacagcgg agcttgctca tgccgcggcc ctccggcggt cgcggccgcg gccgttgcgg
     3481 ccttcgtgct ggttctgcag gaagctcagc agatgattga ccggcggcac aagcatgtcg
     3541 gccccgcgca agagctcgcg ggcgtggtcc aggccgacct tggcccggta aatcaggcgg
     3601 tgggcctctg ccagggcgcg aatgttgtcg gcggaaaaat cgttgcg
//
LOCUS       DASRJB010000941         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2807043, whole genome shotgun sequence.
ACCESSION   DASRJB010000941 DASRJB010000000
VERSION     DASRJB010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2807043"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VFB80_24960"
     CDS             <1..1150
                     /locus_tag="VFB80_24960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN37115.1"
                     /translation="SELSRALVEVGELQGLGRVRDCLEHVVGGQALVLTAGDGGLTAA
                     GMAEGDLDEHERAVADWVFQHGQRAGMGTDTLPSVGALFVPLATPGGVCGVLGVRPRE
                     KVALFSPDQLRLIEAAAGQLALAVERIRAAEAARHALLRYEREQLRSTLLSAVSHDFR
                     TPLAAIAGAGSTLAESGHALTHETRRELAESIVAETERLNRLIGNLLDMTRLEAGSLQ
                     LDRQWHPPGEIVGAVLHRLGGLLEGRQVTVDLPADLPLVYLDELLFHQVLVNLLENAA
                     RYTPREAEINLSAGTRGSAFWLEVSDTGPGLPPGDEERVFEKFYRGANVRTHTGTGLG
                     LAICRGIVELHGGVIRAANRPGGGAAFRIELPQPPQPSVPLPLETAAV"
     gene            1147..1878
                     /locus_tag="VFB80_24965"
     CDS             1147..1878
                     /locus_tag="VFB80_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN37116.1"
                     /translation="MSDAARILVIEDEPEIRRFLRASLAAEHFAAQESETGQAGLRAA
                     AAQPPDVVLLDLGLPDIGGLEVIRQLRGWSRVPIIVLSARGQEADKIAALDAGADDYL
                     TKPFGIGELLARLRVALRHRSSRGETPSQEFTHGAVRVDLVRRRVLVAGQEVHLTPTE
                     YRLLTTLIQHAGKVLTHRQLLKEVWGPDCTHETHYLRVYVNQLRQKLGDDAARPQLLL
                     TEPGVGYRLREEIQNSEFGTQTEDL"
     gene            2018..2437
                     /locus_tag="VFB80_24970"
     CDS             2018..2437
                     /locus_tag="VFB80_24970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN37117.1"
                     /translation="MKEATRPAETPHSLVKAFHGVRYQVSDVSRAVAFYTQHLGFTLK
                     HQKLPAFANVSLGPLDLLLSGPGASGSRPLPGGQSQQPGGWNRIVLHVTDLSAFTEQL
                     RRAGLHFRNQMEVGPHGKQIQLEDPDGNPIELFEPAK"
     gene            complement(2602..>3025)
                     /locus_tag="VFB80_24975"
     CDS             complement(2602..>3025)
                     /locus_tag="VFB80_24975"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HZN37118.1"
                     /translation="RSGCALPDGYRRFLQHVGNGGSGPPSFGLLRLGQTPNDLRAEES
                     RPWVDGTRVSRPFPFTRPWVWEDGDSSDEGTREQVGDGSIHLGTDGCGQYWLLIISGP
                     ERGNVWMLADVGMGPTVPKRDFLQWYEDWLDGVREWWA"
BASE COUNT          514 a         1070 c          966 g          475 t
ORIGIN      
        1 gagcgagctg tcgcgggctc tcgtcgaggt cggcgagttg cagggcctgg gccgagtgcg
       61 cgactgcctg gagcatgtcg tcggcggaca ggcgctggtg ttgacggctg gagacggcgg
      121 actgacggcg gccgggatgg ccgaaggcga tctcgacgag cacgagcggg ccgtcgccga
      181 ttgggtcttt cagcacggtc agcgggcggg aatgggcacc gatacgctgc cgtcggtcgg
      241 ggcgctgttc gtgccgctgg ccacgcccgg cggagtgtgc ggcgtgctcg gcgtccgccc
      301 gcgcgagaag gtggccctct tctcgcccga tcagctgcgg ctgatcgagg ccgccgcggg
      361 ccagctggcc ctggccgtcg agcggattcg cgccgccgaa gcggcgcggc acgcgctgtt
      421 gcgctacgag cgcgagcagc tgcggagcac cctgctctcg gccgtgtcgc acgatttccg
      481 cactccgctg gcggcgatcg ccggcgcggg aagcacgctg gccgaatcgg ggcacgccct
      541 cacccacgaa acgcgccgcg agctggcgga gtcgatcgtc gccgagacgg agcgcctcaa
      601 ccgcctgatc ggcaacctgc tcgacatgac gcggctcgaa gcgggcagcc tgcagctcga
      661 ccgccagtgg catccgccag gcgaaatcgt cggtgcagtg ctccaccggc tcggcggcct
      721 gctggaaggc cgccaagtga ccgtggacct gccggccgat ttgccgctgg tctatctcga
      781 cgagctgctg tttcaccagg tgctggtcaa cctcctggaa aacgccgccc gctatacgcc
      841 gcgcgaggcc gagatcaacc tctccgcggg cacgcgcggc tcggccttct ggctcgaagt
      901 ctccgacact ggaccgggac tgccgcccgg cgacgaagag cgcgtgttcg aaaagttcta
      961 tcgcggggcc aacgtgcgga cgcacaccgg caccggcctg ggcctggcca tttgccgcgg
     1021 catcgtcgag ctgcacggcg gcgtcatacg cgccgccaat cggccgggcg gcggtgcggc
     1081 gttccgcatc gagctgccgc agccgccgca gcccagcgtg ccgctgccgc tggaaaccgc
     1141 agccgtatga gcgacgccgc ccgcatcctg gtgatcgaag atgagccgga aatccgccgc
     1201 ttcctgcggg cgtcgctggc cgccgagcat tttgccgccc aagaatccga aaccggccag
     1261 gccggcctgc gagccgcggc tgcccagccc ccggatgtcg tgctgctcga cctgggcctg
     1321 cccgatatcg gcggactgga ggtgatccgg cagctccgcg gctggtcgcg cgtgccgatc
     1381 atcgtgcttt cggcccgcgg gcaggaggcc gacaagatcg ccgccctcga cgccggggcc
     1441 gacgactacc tcaccaagcc cttcggcatc ggcgagctat tggcccggct gcgggtcgcc
     1501 ctgcggcacc gctccagccg cggcgagaca cccagccagg agtttacgca cggcgcggtg
     1561 cgggtcgatc tggtccgccg ccgcgtgctc gtcgccggcc aggaggtcca cctgaccccg
     1621 accgaatatc gcctgctgac cacgctgatt cagcacgcgg gcaaggtcct cacgcaccgg
     1681 cagctgctga aggaagtctg ggggccggac tgtacgcacg aaacccacta cctgcgggtc
     1741 tacgtcaacc agctccgcca aaagctcggc gacgacgccg cccggcccca gctgctcttg
     1801 accgagccgg gcgttgggta ccggctgcgg gaggagattc agaattcaga attcggaact
     1861 cagaccgagg acctttgaag ccttcaaagc cgcgaccagt ggtcgcgctg cacccgcgct
     1921 ggttcccagg ccctgcctgg aaaacgtccc cgaggctcca gtctcgtttc cgtcggcatc
     1981 aactttgcag tcgtaatcgc gaagggaggc ctcagccatg aaagaagcaa cgcgccccgc
     2041 ggaaacgccc cacagcctgg taaaggcatt tcacggcgtt cgctatcagg tcagcgatgt
     2101 gagtcgcgcc gtggcgttct acacccagca tctgggattc acactcaagc atcagaagct
     2161 cccggccttt gccaacgtct cgctcggacc tctggacctt ttgcttagcg gtccgggggc
     2221 gtccggctcg cgtccgctgc ccggcggcca aagtcagcaa ccaggcggct ggaaccgaat
     2281 cgtcctgcac gtcaccgatc taagcgcctt caccgaacaa ctgcgccgcg cgggactcca
     2341 tttccgcaac caaatggaag tcggtcccca cggcaagcaa atccaactcg aagatccgga
     2401 cggcaatccc atcgaactgt tcgaaccggc gaagtagtca acagattatc cgcgtgggct
     2461 ggccacgaac ttgttcccaa gacttttccc caaccggcca gacacggcgg cgtttggtcc
     2521 cgctgccccg tgaagcccga ttcacgacgc caaccgcgtc aagtattgag gcctgacccc
     2581 tttagtctcc ccatagaaag gttaggccca ccactcccgg acgccgtcca gccaatcttc
     2641 gtaccactga aggaagtctc gcttcggcac cgttggcccc attccgacat cggcaagcat
     2701 ccacacgttg ccgcgctcgg gtccactgat gatcaacaac caatactgac cgcaaccgtc
     2761 cgtgccgaga tgaatgctgc cgtcaccgac ctgctcccgc gttccctcgt cggaggagtc
     2821 accgtcctcc cacacccatg gacgcgtgaa agggaacggc cgggagacgc gtgtgccgtc
     2881 aacccatggc cgcgactcct ccgcccttaa gtcgttgggc gtttgaccca gccgcagcag
     2941 gccaaatgac ggcggcccgc tcccgccatt gccaacgtgc tgaaggaatc tgcggtagcc
     3001 gtcgggcaac gcacagccac tgcgt
//
LOCUS       DASRJB010000942         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_3662609, whole genome shotgun sequence.
ACCESSION   DASRJB010000942 DASRJB010000000
VERSION     DASRJB010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662609"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            974..3703
                     /gene="acnA"
                     /locus_tag="VFB80_24980"
     CDS             974..3703
                     /gene="acnA"
                     /locus_tag="VFB80_24980"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZN37119.1"
                     /translation="MPTPTLFDPFQSRDTFETPSGKAGIYRLERLERQGLGQVSKLPF
                     SMRVLLEAVLRNCDGYEVTEADVRALAAWNAESPAKVEIPFKPARVVLQDFTGVPCVV
                     DLAAMRSAMKRLGGDPKKINALIPVDLVIDHSVQVDYFGSPEALEKNVAVEFERNRER
                     YEFLRWGQKSLANFRAVPPNVGIVHQVNLEYLAKGVFLRSDKLGPVALPDSLVGTDSH
                     TTMINGLGVVGWGVGGIEAEAVMLGQPLYMLMPEVIGFELTGEMKPGVTATDLVLTVT
                     QNLRKVGVVDKFVEFYGSGVSRMSLADRATISNMAPEYGATIGFFPVDAESLNYLRAT
                     GRTKDEVELVERYCKEQGLFRTDATPAPKFTKTVSLDLSTVEPSLAGPKRPQDRVALG
                     AMKETWHKSLRAPVTERGFGLAEGELTKKAAVKDNGHSADITHGAVVIAAITSCTNTS
                     NPSVMLAAGLLAKKAVERGLTVPPYVKTSLAPGSRVVSDYLDQAGLTQPLAKLGFHTV
                     GYGCTTCIGNSGPLPEPVAAAVTEGNLVASAVISGNRNFEGRVNPLVKANYLASPPLV
                     VAYAIAGTTDIDLTKEPIGAGTGGTKVYLKDIWPTTEEVRQTLAKSVLPQMFSSRYAN
                     VFAANEVWNQIKVAEGDLYQWDEQSTYIQEPPFLADMTVAAGRIQPIAGARVLAVLGD
                     SVTTDHISPAGNISKNSPAGKYLMEHGVQPADFNSYGSRRGNDRVMLRGTFANIRIRN
                     FLAPGTEGGVTCYLGKPGETVGPVMPIYDAAMLYKASHVPTIILAGAEYGTGSSRDWA
                     AKGTYLLGVRAVIAASYERIHRSNLVNMGVLPLQFLDGQTWKSLGLTGEETYEIVGLN
                     DSLAPRSKVSVKAVAPGGNETTFIAAVRIDTPVELDYYKNGGILQTVVRKLLA"
     gene            complement(3700..4149)
                     /locus_tag="VFB80_24985"
     CDS             complement(3700..4149)
                     /locus_tag="VFB80_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37120.1"
                     /translation="MSFAILLVLPAWTAAQDSVSADLQPDVAALREKIEAFFTQLAGS
                     MPDAEHAVRAIIGDGPLQSRTDDVKKLIEQAQALDARVGLHKAHEPVSSRNVGSDLLF
                     LRYLYKGEKYPVVWYFTFYRTIGSAGLKSDWKLISLRFDTKVETLDR"
     gene            complement(4170..>5015)
                     /locus_tag="VFB80_24990"
     CDS             complement(4170..>5015)
                     /locus_tag="VFB80_24990"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZN37121.1"
                     /translation="EIAGEKAGIIKPGVPVVSGVLLDEPRLVITRRAAELQAPLWQRG
                     VDYDVATRLGDCSPGGESLDYFEPAAQPRLRVDGVQIQMLGEHQAANAAAAICAVNRL
                     RDQGWTISEAALRQGLVAARTPARIEVVAERPTTILDVAHNPASIAALVAVVNQRWPN
                     ERRILVFASSKDKDFPGMLRLVVPEFDAIVLTRYIHNPRAAETAELLAAATATGVNSR
                     PIEAFDDPGEALARARRLAGPDDLICITGSFFLAAELRPLVIEGKVEAEQIRKSEEPK
                     NRRLE"
BASE COUNT          912 a         1652 c         1609 g          842 t
ORIGIN      
        1 cctggtgttg gtgttgatga tcgcgacgct ccttgcggtg ggctgctcgc aagctccgga
       61 gcctcagaaa accgaagctg aggctcccgc gccgaagccg gccgtcaaaa cgattccgcg
      121 cacgagaatg cccgccccgc cataagccct gcccccgaga ggttcgcatg cttcgcagct
      181 tgggactgat gctattggtc atcctcgtcg gctgttccaa gtccgcgccg ccggccgccc
      241 ccgccgcccc ggaaaccccg ccggcccaaa gcggcggccc ccctgccacg aattccggcc
      301 gcgcaacggc tgtaccagcc agctgacggg cagtcctgta gggtgggctc cgcccaccac
      361 cctcgtccac tatctattgc tatagtggac aagtggcgca gtgacgcgta taatatgtac
      421 acgcccgccc tgccgacccg gcggccgggc ggctctttgg aaatttggca gacgcgtgaa
      481 agcaggcgga ccagcgtttg cgcgcataat gcgcatattg cttcgccgcg gatttgcagc
      541 gcaaccttgg gtgtggcaag gagttaagga cggtttcgac cgcccaggaa aaatgttggc
      601 gacatgcgcg catagccgcg gccccaggag ggcgaggctc ttcccgggag ggcgaggctc
      661 acgctgagcc gcgaatcgca aggaaatcgc tgtgtgtctc agcaggggct tttcgcgctc
      721 ccgaagcaaa gcgcgcataa tgcgcataat cgatgcgcgg cgaaacggat cgcaagtgct
      781 gtctggagct tgagttgcga ccgaaaacgc ccgccgagga tttcttcgca gtatgcgcgc
      841 atacgcgaca gccgaccccc gactcctgac tcccgacccc cgcgaccaat ggtcgcggct
      901 ttggcccatt cgctaaaatc gaacagccgg atatgtctgc ggccaagttt tgacttaccc
      961 ctgcgtgcct gccatgccta cgccgacctt gtttgatccg ttccagagcc gcgacacgtt
     1021 cgagaccccc agtggcaagg ccggcattta ccgactggag cgattggaaa ggcagggcct
     1081 cgggcaggtc agcaagctgc ccttcagcat gcgagtgctg ctcgaggcgg tgctgcggaa
     1141 ctgcgacggc tatgaggtga cggaggccga tgttcgggcg ctggccgctt ggaacgccga
     1201 gtcgccggcg aaagtcgaaa tccccttcaa gccggcccgc gtcgtgctcc aggatttcac
     1261 cggcgtgccc tgcgtggtcg atctggcggc gatgcgctcg gcgatgaagc ggctcggcgg
     1321 cgatccgaag aaaatcaacg cgttaattcc ggtcgatctg gtcatcgacc actcggtgca
     1381 ggtcgattac ttcggcagtc cggaggccct ggagaagaat gtcgcggtcg aattcgagcg
     1441 gaaccgcgag cgctatgagt tcctccgctg gggacagaag tcgctggcca acttccgcgc
     1501 cgtgccgccc aacgtcggca tcgtgcacca ggtgaacctc gagtatctcg ccaagggcgt
     1561 cttcttgcgg agcgacaagc tcggaccggt cgccctgccc gattcgctcg tcggcaccga
     1621 cagccacacc acgatgatca acggcctggg cgtcgtgggc tggggcgtcg gcggcatcga
     1681 ggccgaagcc gtgatgctcg gacagccgct ctacatgctg atgcccgagg tgatcggctt
     1741 cgaactgacg ggcgagatga agccgggtgt gacggcgacc gacctcgtgc tcaccgtcac
     1801 gcagaacctg cgcaaagtcg gcgtggtcga taagttcgtc gagttttatg ggtcaggcgt
     1861 gagccggatg tcgctcgccg accgggcgac catcagcaac atggcgccgg agtacggggc
     1921 gacgatcggc tttttcccgg tcgatgccga gtcgctgaat tacctgcggg cgacgggccg
     1981 cacgaaggat gaagtcgagc tggtcgagcg ttactgcaaa gagcagggtc tgttccgcac
     2041 cgacgccacg ccggcgccga agttcacgaa gaccgtctcg ctcgaccttt cgaccgtcga
     2101 gccgtcgctg gccgggccaa aacgcccgca ggaccgcgtg gctttaggcg cgatgaaaga
     2161 gacctggcac aagtcgctgc gagcgcccgt caccgaacgc ggcttcggcc tcgccgaagg
     2221 agagctgacc aagaaagcag ccgtcaagga caacgggcac tccgccgaca tcacgcacgg
     2281 cgcggtcgtc atcgcggcga tcaccagctg caccaacacc agcaatccgt cggtcatgct
     2341 tgccgcgggg ctgctggcga agaaggcggt cgagcgcggc ctgaccgtgc cgccctatgt
     2401 gaagaccagc ctcgcgccgg gctcccgcgt cgtcagcgac tacctcgatc aggccggcct
     2461 tacgcagccg ctggccaagc tcggctttca caccgtcggc tacggctgca cgacctgcat
     2521 cggcaacagc ggcccgctgc ccgagccggt ggccgccgcc gtcaccgaag gcaacctcgt
     2581 ggcgtccgca gtcatttccg gcaaccgcaa tttcgagggc cgcgtcaacc cgctggtcaa
     2641 ggcgaactac ctggccagcc cgccgctggt cgtcgcctat gccattgccg gcacgaccga
     2701 catcgacctg accaaggagc cgatcggcgc agggactggc gggacgaagg tctatctgaa
     2761 ggacatctgg cccacgaccg aggaagtgcg gcagacgctg gccaaatcgg tgctgccgca
     2821 gatgttctcg tcgcgctatg cgaacgtctt tgccgccaat gaggtctgga accagatcaa
     2881 ggtggccgag ggcgatctct atcagtggga cgagcagagc acctacatcc aggagccgcc
     2941 atttctggcc gatatgaccg tggccgcggg gcggattcag ccgatcgccg gcgcgcgggt
     3001 gctggcggtg ctcggcgaca gcgtgacgac cgatcacatt tcgcccgcgg gcaacatcag
     3061 caagaacagc cccgccggaa aatacctcat ggagcacggc gtgcagccgg ccgacttcaa
     3121 cagctacggc agccggcggg gcaacgaccg cgtgatgctc cgcggcacgt ttgcgaacat
     3181 ccgcatccgg aatttcctcg caccgggcac cgaaggcggc gtgacctgct atttgggcaa
     3241 gccgggcgag acggtcggcc cggtgatgcc gatctacgac gccgcgatgc tctacaaggc
     3301 gagccatgtg ccgacgatca tcctcgccgg cgcggaatac ggcaccggca gctcgcgcga
     3361 ctgggcggcc aagggcacgt atctgcttgg cgtgcgggcc gtcattgccg ccagctacga
     3421 gcggatccac cgcagcaacc tggtcaacat gggcgtcctg ccgctgcagt tcctcgatgg
     3481 ccagacctgg aaatcgctcg gcctgaccgg cgaagagacc tacgaaatcg tcggcctgaa
     3541 cgattcgctt gcgccgcggt ccaaagtcag cgtcaaggcg gtcgcgcccg gcggcaacga
     3601 aacgacgttc atcgccgccg tccgcatcga cacgcccgtt gagctggact actacaagaa
     3661 cggcggcatc ctgcaaacag tggtgcggaa gctgctggct tagcggtcca gtgtttccac
     3721 cttcgtatcg aaccgcagcg agatcagctt ccagtcgctc ttcaggccgg cggagccgat
     3781 ggtgcgataa aacgtgaagt accagaccac cggatacttc tcgcctttgt agagataacg
     3841 caggaagagc aggtcgctgc cgacattgcg gctgctgacg ggctcgtgcg ctttgtgcag
     3901 gccgacgcgg gcgtcgagcg cctgggcctg ttcgatcagc tttttgacgt cgtcggtgcg
     3961 cgattgcagc gggccgtcgc cgatgattgc ccgcacggcg tgctcggcgt cgggcattga
     4021 tccggccagc tgggtgaaga acgcctcgat cttctcccgg agggccgcca cgtccggctg
     4081 caagtcagca ctgacgctgt cctgcgccgc ggtccacgct ggcagcacca gcaggattgc
     4141 aaagctcaac gacaatgtcc aacgcgctgt cattcgagtc ttcggttctt cggttcttcc
     4201 gatttgcgaa tttgctccgc ctccactttg ccttcgatca ccagcggccg caactcggcg
     4261 gccagaaaaa acgagcccgt aatgcagatc aggtcgtccg ggccagccag ccgccgggcg
     4321 cgggccaggg cctcgccggg gtcgtcgaac gcttcgatcg gccgcgaatt gacgccggtc
     4381 gcggtcgccg cggcaagcag ctccgccgtt tccgccgctc gtgggttatg gatgtagcgc
     4441 gtcagcacga tggcatcgaa ttcgggcacg accagtcgca gcatgccggg gaagtctttg
     4501 tccttgctgc tggcgaagac gagaatccgg cgctcgttgg gccagcgctg attgacgacg
     4561 gccacgagcg ccgcgatcga ggccggatta tgcgctacat cgaggatagt cgtcggccgc
     4621 tcggcgacaa cttcgatgcg ggccggcgtg cgggcagcga ccaggccctg ccgcagcgcc
     4681 gcctcggaaa tcgtccagcc ctggtcgcgg agccgattca cggcgcaaat cgccgccgcc
     4741 gcgttcgccg cctgatgctc gcccagcatc tgaatctgga cgccatctac gcgaagtcgt
     4801 ggctgagcgg ccggctcgaa atagtccagc gactcgccgc caggcgagca gtctcccaga
     4861 cgggtagcga cgtcatagtc cacgccgcgc tgccagagcg gagcctgcag ctccgccgcg
     4921 cggcgcgtaa tcacgagccg cggttcgtcc agcagcacgc ccgaaaccac tggcacgccg
     4981 ggcttgatga tccctgcctt ttcaccggca atctc
//
LOCUS       DASRJB010000943         7496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_845115, whole genome shotgun sequence.
ACCESSION   DASRJB010000943 DASRJB010000000
VERSION     DASRJB010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845115"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1202)
                     /locus_tag="VFB80_24995"
     CDS             complement(165..1202)
                     /locus_tag="VFB80_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37122.1"
                     /translation="MPPRHYSYLGALALAIASAFAQPARAANPRVELEIASEPGFPVA
                     DGRAWTEMLTKAGFATVRIRGGKPDDKLAIQTRGTEAAPSYLVTGALTADGQLQLLKG
                     RFGLGDRAKIEAWLAKLRDGGQEGITVKPEAFGLLPKQLVAVHEALAVPVGFSTKGKR
                     PRDVARQIADTLSLKFITDTVGQAALASEEPVADELQGVSAGTALAAVLRPLGLVLVP
                     EKSGSEIRLRISDSRAAKENWPVGWPVKSNPGTTLPGLFKFLDVEIEQTPLSEAIAAI
                     GGRLKVPLLIDHNSVARKAVDLNMKVNLPKGKTFYGRALDQLLYQAKMKSEVRIDEAD
                     RPFLWITAGGP"
     gene            1481..2746
                     /locus_tag="VFB80_25000"
     CDS             1481..2746
                     /locus_tag="VFB80_25000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN37123.1"
                     /translation="MPQPREVVITGLGVVSPVGVGREAFWQALEAGTSGVDWLPETKG
                     LDLPFRYAARIKDFDAKQYVQPRKTIKVMCPEIQSAYAAAAMAVEDGGLAKGAVDPDR
                     MGVVLGSETLYGDLDELVECYRHCAPEGQFHMAKWGEFAFKDLFPLWMLKYLPNMAAC
                     HISIAHDARGPNNSIVEGGASSLLAVGEAAAAIERGHADAMLAGGSGSATAFSCMPFR
                     GWEQLATWQGEPAAASRPFDARRTGLVPGAAAGVLLLEAREAAQARGAKILARLASWS
                     SRYEPPVSRVQPGQRAIRQSIEAALALAKMRPADIGHVNAHACSSIAGDRAEAQAIAA
                     ALGDVPVTALKSYFGDCAAGSGAVELIGSVLGLVHGRVPRTLNYEEPDPECPVNVVAG
                     SPAPVKQPTAIALNHSVTGQAAAVIIVRD"
     gene            2767..2943
                     /locus_tag="VFB80_25005"
     CDS             2767..2943
                     /locus_tag="VFB80_25005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37124.1"
                     /translation="MAKNRKHSAKTSSSQSPKAPAPATLAAHPPARHPTLLAVSVALF
                     VLWFIFLLVTALAA"
     gene            3176..3676
                     /locus_tag="VFB80_25010"
     CDS             3176..3676
                     /locus_tag="VFB80_25010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN37125.1"
                     /translation="MTTIPREPAEILLIDEDAAAMAAAAAVLEAAGYHVYQAADRTAA
                     LKIGRQQALDLIISDVNLGGKSGLELCRDLRRLPGMLDVPVMFVSSMQVPDIVRRSHD
                     AGGAYYLRKPLDPEVLVDLVGKALWMPHLVQSRLAMHDSVVQPVPPPQHNSRAAAVQG
                     IRVPLA"
     gene            complement(3742..5133)
                     /locus_tag="VFB80_25015"
     CDS             complement(3742..5133)
                     /locus_tag="VFB80_25015"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HZN37126.1"
                     /translation="MSQLNRRQFAAASAAVLTASTMPHVRAANEAPDWRLGSFAADIT
                     PPLGHPLIGGLREPAKSIKDRLSARGVVLGGPAKPLVICGLDWCELRNDAYDRFRDAL
                     AIATGTDRQRVLLTCLHQHDAPYFDLAGQKLLDAAGYPKLMFDPAFFEQAVADTAAAA
                     KLALTKLRPVTHIGVGQAAVEKVACNRRVVGADGKPRFNRYSFVRDPAVRDAPEGLID
                     PELKTLSFYSGEEPLAAISLYATHPMSYYGNGEVSYDFPGMAREMRQRQMPDVFQVYL
                     TGCSGDVVAAKFNDGTPAGRQQLAQRLLDGMNAAWKATRRNPLTDIKFRCPPLELPPP
                     DEGKLTIPVLEKTIADPAASSSDRIHAALGLSYRRRCDRGQPIDVPAIDFGAAQIVVL
                     PAELFVGYQLAAQKMRPEQLLLTPGFGECAPGYTPTDSARREGFVEEHGYCWVQPGVE
                     ERILAAIRQAITP"
     gene            5349..5576
                     /locus_tag="VFB80_25020"
     CDS             5349..5576
                     /locus_tag="VFB80_25020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37127.1"
                     /translation="MDAAMLREATRYHAGHSMTEMGASRLVPAAEFRKLLVSHRRMVR
                     ADRKDQRLRGLQDLDTGEVFLTDERRLLEAR"
     gene            complement(5573..6643)
                     /locus_tag="VFB80_25025"
     CDS             complement(5573..6643)
                     /locus_tag="VFB80_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37128.1"
                     /translation="MADSQPNRLPLLRLLLAGMAVPAACAALDHALLTQLMMGTANGS
                     VMVLTLAAFIFQVGLFGVMCGKFIDHPVLRWAIYIWCWVLVDLQCLTAGSLAGPSYYW
                     GNMQRLLPTSLFNAQLGLVTIWAVLGTLRWYFRLPAALVLATLCALPLLDSHYHGSEL
                     GGLFFMQTCVLAFICGVLRWQRFRLVQPVKLAAEARGDLTRLQFGVRHVLIWTTSLAV
                     VLALARALNVLSPGFVAEMLGQRWLVNVTAGGLIAVVLVLALWAGLGEGSWLRLLALA
                     IVAPLAGTLVAASEWYNHWHTPQGMWAVNPPLWSSAYWNGWWDTEWWLCAWTCLAGGL
                     LFAALLIYRTLGYRLTRTRRAA"
     gene            6726..7457
                     /locus_tag="VFB80_25030"
     CDS             6726..7457
                     /locus_tag="VFB80_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="platelet-activating factor acetylhydrolase IB
                     subunit"
                     /protein_id="HZN37129.1"
                     /translation="MNASRRLLIAAAAVCCLPAWCLAQDKPANDAVVAAPREGGWMTR
                     HNSFNERVKKGNVDLLFIGDSITQGWEGQSPRGGQKVWEEFYGKRNAVNLGIGGDRTQ
                     HVLWRLDHGNIDGIKPKLAVLMIGTNNSGTNTSEQIAAGVKAIVEKLRMKLPETKVLV
                     LAIFPRGADKAAKQREVNEGANAIIKGLADEKMVYYLDIGPKFLAEDGTLSQEVMPDR
                     LHLNEKSYRTWAEAIEPSVKKLMGE"
BASE COUNT         1293 a         2441 c         2575 g         1187 t
ORIGIN      
        1 tgcggacaag cagttacgct cagcgccgcg ggcctcaggg gccttgcaaa aacccgaagc
       61 tgcgggaatt atccttaccc tgtgaagcgc caattcggtg cgacgcaccg gcgagcgaag
      121 ggcggggagc cctcgccccg gccctctccc agggagaggg agcctcacgg tcccccggcg
      181 gtgatccaca agaacggccg gtcggcttcg tcgatgcgga cttcggattt catcttggcc
      241 tggtacagca gctggtcgag cgcgcggccg tagaacgttt ttcctttggg gaggttgacc
      301 ttcatattca gatcgacggc cttgcgggcg acgctgttgt ggtcgatgag gagcgggact
      361 ttcaggcggc cgccgatggc ggcgatggct tcggagagcg gcgtttgttc gatctcgacg
      421 tcaaggaact tgaagagacc gggcagcgtc gtgccggggt tcgatttcac gggccagccg
      481 acgggccagt tttccttcgc cgcgcggctg tcgctgatcc gcaggcggat ctcgctgccg
      541 gatttttccg gcacgagcac caggcccagc ggccgcagca cagcggccag cgcggtgccc
      601 gccgagacgc cttgcagctc gtcggcgacc ggctcttcgc tggccagggc cgcctggccg
      661 acggtgtcgg tgatgaattt gaggctgagc gtgtcggcga tttgcctggc gacgtcgcgc
      721 ggccgcttgc ctttcgtcga gaaccccacg ggcacggcca gcgcctcgtg gaccgcgacc
      781 agctgcttgg gcaacaggcc gaacgcctcg ggcttgacgg taattccttc ctggccgcca
      841 tcgcgcagct tcgccagcca ggcctcgatc ttggcccggt cgccgaggcc aaagcggccc
      901 ttgagcagtt ggagctggcc gtcggcggtg agtgcgccgg tcaccagata cgacggtgcg
      961 gcctccgtgc cgcgggtctg gatcgccagc ttgtcgtcag gcttgccgcc gcggatgcgg
     1021 accgtggcga accccgcctt ggtgagcatc tcggtccagg cccggccatc ggcgaccgga
     1081 aagcccggct ccgaagcaat ctccagctcg acgcgcgggt tggcggcgcg ggcgggttgc
     1141 gcgaatgcag aagcgatcgc cagcgcgagt gcgccaagat agctgtaatg gcgaggcggc
     1201 atgttccggc tctccgaatg ccgacgacgc tggcagcgtc gtcaacggcg cccaccttca
     1261 tcctacttca tgcgggcggc tgcggcccac ccgtgcaagc cttgccgcca gccgcagggg
     1321 gcgatagcat cgactgcgtc gtctcatcgc ggcggtcacg ctagcgattc cagcgacttt
     1381 gctgccggag cgtccgccgg agcttgcatt cagcccttcc gccgcgtcga tagcatttca
     1441 atcccggcca ctggcgatcc gtccctgcat cacccgtccc atgccccagc cccgcgaagt
     1501 cgtgatcacc ggactgggcg tggtgtcgcc ggtgggcgtc ggccgcgagg cgttctggca
     1561 agccctcgaa gcgggaacaa gcggcgtcga ttggctgccc gagacgaagg ggctcgacct
     1621 gccgtttcgc tacgcggccc gcatcaagga ttttgacgcc aagcagtacg tgcagccgcg
     1681 aaagaccatc aaggtcatgt gccctgagat tcagtcggcc tatgcggctg cggcgatggc
     1741 cgtggaggac ggcgggctgg cgaagggggc ggtcgatccg gaccggatgg gcgtcgtgct
     1801 gggaagcgag acgctgtacg gcgacctcga cgagctggtc gagtgctacc gccactgtgc
     1861 tccggagggg cagtttcaca tggcgaagtg gggcgagttt gccttcaagg acctgttccc
     1921 gctgtggatg ctcaagtacc tgccgaacat ggcggcctgc cacatcagca tcgctcacga
     1981 cgcccgcgga ccgaataact cgatcgtcga gggcggcgcc tcgagcctgc tggcggtcgg
     2041 cgaggcggcg gccgcgatcg agcgcgggca cgccgacgcg atgctcgccg gcggcagcgg
     2101 ctccgccacc gcgttcagct gcatgccgtt tcgcggctgg gagcagctgg cgacctggca
     2161 gggcgagccc gccgcggctt cgcgaccgtt cgatgcccgc cgcacggggc tggtgcccgg
     2221 cgcggcggcc ggcgtgttgc tgctggaggc ccgcgaggcg gcccaggccc gcggggcgaa
     2281 gattctcgcg cggctggcca gctggtccag ccgctacgag ccgccagtat cccgcgtgca
     2341 gccggggcag cgggcgatcc gccaatcgat cgaggcggcg ctggccttgg cgaaaatgcg
     2401 gccggcggac atcggacatg tgaacgcaca cgcctgcagc tcgatcgccg gagaccgcgc
     2461 cgaagcccag gcgattgctg ccgcacttgg cgacgtgccg gtcacggccc tcaagagcta
     2521 tttcggcgac tgcgctgccg gcagcggcgc ggtggagctg atcggcagcg tgctggggct
     2581 ggtgcacggc cgcgtgccgc ggacgctcaa ttatgaagag cccgatccgg agtgcccggt
     2641 gaatgtcgtc gccggctcgc ctgcgccggt caagcagccg acggccatcg cgctcaatca
     2701 ttcggtcacc ggccaggctg cggcggtgat tatcgtcagg gactgaacca ctcgggaggg
     2761 aatccgatgg ccaaaaaccg gaagcacagc gcgaaaacca gctcgagcca atcgcccaag
     2821 gcccccgcgc cggcgacgct cgccgcgcat ccgccggcca ggcatccgac gctgctggcc
     2881 gtcagcgtgg ccctgttcgt gctttggttc atcttcctgc tggtgaccgc gctggcggca
     2941 tagtagtcct cacgctccgc gtgaggcgag catctcgcgg agcgagatga ctactgtgcc
     3001 ggtcgtggcg aacatgccgg tcgcaagggg cggcgaagga ttgctggctc gcggcaggcg
     3061 gatgcgacga tagttttaga tgccagacag gcggtctgca ttttggcccg cacacccgcg
     3121 ggccgccgat ggagcggcga actttcccac caccgattgg gagcggtcgc tgccgatgac
     3181 gacgattccc cgcgaaccgg ccgaaatcct gctgatcgat gaagacgcgg ccgccatggc
     3241 tgcggccgcg gcggtcctgg aggccgccgg ttaccacgtc tatcaggcgg cggaccgcac
     3301 ggctgctctg aagatcggcc gccagcaggc cctcgacctg atcatcagcg acgtgaacct
     3361 cggcggcaaa agcggcctgg aattgtgccg cgatctgcgg cggctgccgg gcatgctcga
     3421 tgtccccgtg atgttcgtga gcagcatgca ggtgcccgac attgtccgcc gttcgcacga
     3481 cgcgggcggg gcctattacc tgcgcaagcc gctcgatccc gaagtgctgg tggacctggt
     3541 gggcaaggcc ctctggatgc cgcacctggt gcagagccgc ctggcgatgc acgattcggt
     3601 ggtgcagccc gtgccgccgc cgcagcacaa ctcgcgggcc gcagcggtgc aggggattcg
     3661 cgtgccgctg gcctaacttg atcgtagccg aactcgcccg agttcggagc catcggaatc
     3721 accgaattct ggcgaattcg gctatggcgt gatcgcctgg cggatcgccg ccaggattcg
     3781 ctcctccacg cccggctgca cccagcagta gccgtgctct tccacaaagc cttcgcggcg
     3841 ggcggaatcg gtcggcgtat agcccggagc acactcgccg aaacccggtg tgagcagcag
     3901 ctgttccggt cgcattttct gggcggccag ctgatagccg acgaacaact cggccggcag
     3961 gacgacgatt tgtgccgcgc cgaaatcgat cgccggcacg tcgatcggct ggccgcggtc
     4021 gcagcggcgg cgatacgaaa ggcccagcgc ggcgtggatg cggtcggagc tgctggccgc
     4081 cggatcggcg atcgtctttt ccagcaccgg gatcgtcagc ttgccttcgt cgggcggcgg
     4141 caattccagc ggcgggcagc gaaacttgat gtcggtgagc ggattgcggc gggtcgcttt
     4201 ccacgcagca ttcatcccgt ccaagagccg ctgggccagt tgctggcgtc cggcgggcgt
     4261 gccgtcgttg aacttcgcgg ccacgacgtc gccgctgcac ccagtgagat aaacctgaaa
     4321 cacatccggc atctggcgct ggcgcatctc gcgggccatg ccgggaaagt cgtaggagac
     4381 ctcgccgttg ccgtaatagc tcatcgggtg cgtcgcgtac aggctgatcg ccgccagcgg
     4441 ctcctcgccg ctgtagaagc tgagcgtctt gagctctgga tcgatcagtc cctctggggc
     4501 gtcgcggacc gccggatcgc ggacgaagct atagcgattg aaccgcggct tgccatccgc
     4561 gccgacgacg cggcggttgc aggcgacctt ctccacagcc gcctggccga cgccgatgtg
     4621 cgtcacgggc cgcagcttcg tcaaggcgag cttcgccgcc gcggcagtgt ccgcgacggc
     4681 ttgctcgaag aatgccgggt cgaacatcag cttgggatag ccggcggcgt cgagcagttt
     4741 ctggccagcg aggtcgaaat acggcgcgtc gtgctggtgc aagcaagtta gcaagactcg
     4801 ctgccggtcg gtgccggtgg cgatcgccag cgcgtcgcga aagcggtcgt aggcgtcgtt
     4861 ccgcagctcg caccagtcca ggccgcagat caccagcggt ttggccgggc cgcccagcac
     4921 gaccccccgg gcggacagcc gatccttgat cgacttcgcc ggctcccgca gaccgccgat
     4981 gagcgggtgt cccagcggcg gcgtgatgtc ggcagcaaag cttcccagcc gccagtccgg
     5041 cgcttcgttc gccgcgcgaa cgtgcggcat ggtactagca gtgagcacgg ccgccgaagc
     5101 agcggcgaat tggcgacggt tcaactggct catggctggc tccgatcgcg gtgttgcgag
     5161 tgctagcaga tgacatgcta tcagtggcca atcgcagcgc caaaaagacg ccctttttgc
     5221 cgccgtaact tgtttccaga aaaatactta aggccaactt ccagagtggg gggttcaggt
     5281 cgtttcgccc cgccggtaca atcccctgac atgaagattc gtggtgctag cgccggaagg
     5341 agcaattcat ggatgcggcc atgctacggg aagcaacacg atatcacgcg gggcattcga
     5401 tgacggaaat gggcgccagc cggctggtcc cggcagcgga gtttcgcaag ctgcttgttt
     5461 cgcaccggag gatggtccgg gcggatcgca aggatcagcg cctgcgcgga ttgcaggacc
     5521 tggataccgg tgaggtcttt ctgaccgacg agcgccggtt gctcgaagcc cgctaagcgg
     5581 ctcgccgcgt gcgagtcagg cggtagccga gcgtgcggta gatcaagagc gcggcaaaca
     5641 gcagaccgcc cgcgagacag gtccaggcgc aaagccacca ttcggtatcc caccagccgt
     5701 tccagtaggc cgacgaccag aggggcggat tgaccgccca cattccctgc ggcgtatgcc
     5761 agtggttgta ccactcgctg gcggcgacga gcgttccggc cagcggcgcg acgatcgcca
     5821 gcgccagtaa gcggagccag ctaccctcgc ccagaccggc ccagagggcg agcacgagca
     5881 ccacggcgat caagcctccg gccgtgacat tgaccagcca ccgctggccg agcatttccg
     5941 ccacgaagcc cggcgagaga acgttgagcg cgcgggccag cgccagcacg acggccagcg
     6001 aagtcgtcca gatcagcacg tggcggacgc cgaactgcag ccgcgtcaga tccccgcgag
     6061 cttccgcggc gagcttcacg ggctggacca ggcgaaagcg ctgccagcgc aggacgccgc
     6121 agatgaacgc cagcacgcaa gtctgcatga agaacaggcc gcccagctcg ctgccgtgat
     6181 agtgcgagtc gagcagcggc aacgcgcaga gcgttgccag gacgagcgcc gccggcaggc
     6241 ggaagtacca gcgcagcgtt ccgagcacag cccagatcgt caccaggcca agctgggcat
     6301 tgaacagcga cgtcggcagc aggcgctgca tgttgcccca gtagtacgac ggccccgcca
     6361 aggagccggc agtcaggcac tgcaaatcga cgagcaccca gcaccagatg tagatggccc
     6421 agcgcagcac tggatggtca atgaatttgc cgcacatcac gccgaacagg ccgacctgga
     6481 aaatgaacgc ggcgagcgtg agcaccatca cgctgccgtt ggccgtgccc atcatcaatt
     6541 gcgtgagcag cgcgtgatcc agcgcggcgc aggccgccgg cacggccatg ccggccagca
     6601 gcaggcggag gagcggcaga cgattgggct gcgaatcggc catgaacgac cactatatcc
     6661 cgtccgcggc agcgggtatc gtggcggttt tccgcatccg gatttcccac ctattggagc
     6721 cactcatgaa tgcctctcgc cgtctgctga ttgcggctgc ggccgtctgc tgtctgcctg
     6781 cctggtgcct ggcccaggac aagcccgcca acgacgccgt cgtggccgcg ccgcgcgagg
     6841 gcggctggat gacgcggcac aattcgttca acgagcgcgt caagaagggc aacgtcgatc
     6901 tgctgttcat cggcgactcg atcacgcagg gctgggaagg ccaatctccg cggggcgggc
     6961 aaaaagtctg ggaggagttc tatggcaagc ggaacgccgt gaacctgggc atcggtggcg
     7021 accgtacgca gcacgtgctg tggcggctcg accacggcaa catcgacggc atcaagccga
     7081 agctggcggt gctgatgatc ggcaccaaca actccggtac gaacacctcg gagcagatcg
     7141 ccgccggcgt caaggcgatc gtcgagaagc tccgcatgaa gctgccggag acgaaggtgc
     7201 tcgtgctggc cattttcccg cgcggggccg acaaggcggc taagcagcga gaggtcaacg
     7261 aaggcgccaa cgcgatcatc aagggcctgg ccgacgagaa gatggtctat tacctcgaca
     7321 ttgggcccaa attcctggcc gaggacggca cgctcagcca ggaggtgatg cccgaccggc
     7381 tccacctgaa cgagaagagc taccgcacct gggccgaggc gatcgagccg agcgtgaaga
     7441 agctgatggg agagtagccg acgctgccag cgtcggcggc gacgcaagct gcgtcg
//
LOCUS       DASRJB010000944         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1407803, whole genome shotgun sequence.
ACCESSION   DASRJB010000944 DASRJB010000000
VERSION     DASRJB010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407803"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VFB80_25035"
     CDS             complement(<1..1579)
                     /locus_tag="VFB80_25035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37130.1"
                     /translation="MTPLTETAVPLDDVRQPARRVSLRWLLALAAAVASALSIACWWQ
                     ERESASRRLAAWEAEQAELAASDGLERLVTLKGGKLTLEAFAELMEREVGLPVEIDFA
                     AIEAEKNIPPRKTLFVRAPTGTLSIRSAFRLALSPLGLAADRREGQIVITTLGESAEE
                     NRLLTVVYPLPQPEPVGMDEDAWRTAVAGCARGDGRHIQAVPGGLVVVSTADGHRRVQ
                     QTIDAICGLAGPLSQPVVVPPQPPGDVERRILAELRQPAQIEFVETPLADAATYLSDR
                     HAIPIILQEKALTEAGINLDAPVTKMLNGVSLRSALRLLLKDLDLTFDVRDEALLITT
                     YEEAESRLHTVAYPVHDLVASAAGPDYAPLTGLVQETIAPQSWEQVGGPGGIRGISGG
                     WLLVSQTAYTQEQVANFLAQLRAALRVDAPRGVVRVSPPSANEQKIERALARTIPLEF
                     QGAPLKDAMQEIQDTLDIPCVINTKTLNDAGINIDTPVTCSLPPGRAGFQLQLLLEPL
                     DLNYVVRDEVLQITTPDD"
     gene            complement(1576..3723)
                     /locus_tag="VFB80_25040"
     CDS             complement(1576..3723)
                     /locus_tag="VFB80_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37131.1"
                     /translation="MRTRLLLVAVSLASIAVIAGLSYFYQQRQTAARRLAAWRELQTD
                     RQSDRLVVAVHPLPPLESSADAEQWVELIKYTIAPHTWDDRGGPAHLTVVPGGLVVRH
                     TTEVQRQIATVFSAFADRDEQPQSLEPLWLLPDKLGALERRIHSALAEPSALDCVDRP
                     LCEVVQELSRRHAIPIALNLKCLDDAGIDPAMRVTMQLRGLTLRSLLRLLLRKCDLTY
                     VVRNGLLQISTPDDADCQEVLVAYPVGDLATPDEPALGFLVDLITTCVAPQSWGFSGP
                     TPDFAFGDHWLVIPQSPEVHERIADFLTRLRRKLSPAEADVLWPLTVAEQNEQRIAAA
                     LDRPLALRLENTPLEAACRQLQSELGVPVVIDERSVRHEEPASRLPVSCDPTAGPARD
                     VLRAMLAQRGLNFRTANEVIEIVSSDYREDPPTQIFDVRPLLTTFSERRLPELIRTLA
                     TPKLVATGGDYGWIDEFRGLLIVRETEANVRRVGRVIQALNALGRTEHKAGQSKPWVI
                     PLAPETGLDHLERLLQTPASVDFCGLPLDLALAQLAAEYDFRVWLNPRSIGGDERFYQ
                     WPPVYLETSELTLGNVLSAMLWPLGLDYMISDGVICVSDPPDAGLFDPRLYRIPVGKD
                     RQLWLARLKLLDGVTTNPPWDPQHGLAIGTPVTPDWFAISADWQGHQRIADWLFEQRT
                     GQTPPWAIQRRRLEAEFPRRLDAASADEDSFTK"
     gene            4163..>4651
                     /locus_tag="VFB80_25045"
     CDS             4163..>4651
                     /locus_tag="VFB80_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZN37132.1"
                     /translation="MSRIVLAMSGGVDSSVAAHLLKEQGHEVIGVFMRHGEEAQDACA
                     ADGPPGFKLPIFDSRADHKQGCCSASDAEDARRVADRLDIPFYALNLQAEFRRIVDYF
                     IDEYSRGRTPNPCVQCNNWLKFGKLFDYADSVGAEFVATGHYARLSSRHTPCAVLLSA
                     GNG"
BASE COUNT          854 a         1485 c         1497 g          815 t
ORIGIN      
        1 agtcgtccgg cgtcgtgatc tgcaggactt cgtcccgcac gacgtagttc aggtcgagcg
       61 gctccagtag gagctgcaat tgaaagccgg cccggcccgg cggcaggctg caggtgacgg
      121 gcgtgtcgat gttgattccg gcgtcgttca gtgtcttggt gttgatcacg cagggaatgt
      181 cgagcgtgtc ctgaatctcc tgcatggcgt ctttgagcgg agctccctga aattccagcg
      241 ggatcgtgcg cgccagcgcc cgctcgatct tctgctcgtt ggccgagggc ggcgagactc
      301 gcacgacgcc gcgcggagca tcgacccgca gcgccgctcg cagttgggca aggaagttgg
      361 cgacttgctc ctgagtgtag gccgtctgcg aaacgagcag ccagccgccg ctgatgccgc
      421 gaattccgcc ggggccgccc acctgttccc acgattgcgg ggcgatcgtt tcctgcacca
      481 gtccggtcag cggcgcatag tccggaccgg ccgcggaggc taccaggtcg tggaccggat
      541 aggcgacggt gtgcaagcgc gactcggctt cttcgtatgt cgtgatgagc agcgcctcgt
      601 cgcgcacgtc gaatgtcaga tcgaggtctt tcagcagcaa tcgcagcgcg gagcgcagcg
      661 agacgccgtt cagcattttg gtgaccggcg cgtccaggtt gatgccggcc tcggtcagcg
      721 ccttctcttg aaggatgatc ggaatcgcat gccgatcgga cagataggtg gcggcatcgg
      781 cgagcggcgt ctcgacgaac tcgatttgcg ctggctgccg tagctctgcc agaattcgcc
      841 tctcgacatc gccgggcggc tgcggcggca caaccaccgg ctgcgacaat ggtccagcca
      901 agccgcaaat ggcatcgatc gtctgctgca cgcggcggtg gccgtcggcc gtcgagacca
      961 caaccagccc gcccggcact gcttgaatgt gccttccgtc gccgcgagcg cagccggcga
     1021 ccgcagtccg ccaggcatct tcgtccatcc ccaccggctc cggctgcggc agcggataga
     1081 cgacagtcag caggcggttt tcctcggccg attcgcccag cgtcgtgatg acaatctgtc
     1141 cttcgcgcct gtccgccgcc agtcccagcg gggaaagcgc caggcgaaag gccgagcgaa
     1201 tcgagagcgt ccccgtgggg gcccgcacga acagcgtctt gcgcggcggg atgttcttct
     1261 ccgcctcgat cgccgcgaag tcgatttcca cgggcagtcc aacctcgcgc tccatcagct
     1321 cggcgaatgc ctccagcgtc agtttcccgc ccttcagcgt caccagccgc tccagcccgt
     1381 cgcttgccgc cagctccgcc tgctccgcct cccacgccgc cagccggcgg gaggcggact
     1441 cgcgctcctg ccaccaacag gcgatggaca gtgcactcgc aacggccgcg gccagcgcga
     1501 gaagccagcg gagcgaaact cgacgggcgg gctgccgaac gtcgtcgaga ggaacggcag
     1561 tttcagtcaa cggggtcatt tcgtgaacga atcctcgtct gcggatgccg cgtcgagcct
     1621 ccgcggaaac tcagcctcca ggcggcggcg ctggattgcc cagggcggcg tctgccccgt
     1681 gcgctgctcg aataaccaat cggcgatccg ctgatggcct tgccagtcgg cgctgatggc
     1741 aaaccaatcg ggcgtgaccg gcgtgccgat ggccaaacca tgctgcggat cccaaggtgg
     1801 attggtggtg acgccgtcga gcagcttcag cctggcgagc cagagctggc gatccttgcc
     1861 gacggggatg cgatacagtc gcggatcgaa caggccggca tcgggcgggt ctgagacgca
     1921 aatcacgccg tccgaaatca tgtagtccag gcctagcggc caaagcatgg cgctgagcac
     1981 attgcccaaa gtcagctcgc tggtttccag atagaccggc ggccactgat aaaagcgctc
     2041 gtcgcctccg atcgaacggg ggttcagcca gacgcggaag tcgtattcgg cggcgagttg
     2101 tgccagcgcc aggtcgagcg gcaggccgca aaaatcgacg ctcgccggcg tctgtagcag
     2161 ccgctcgagg tggtcgaggc cggtctcagg cgccaacggt atgacccaag gcttcgactg
     2221 gcccgcctta tgctccgtcc ggcccaaggc attcagagct tgaatcactc ttcccacgcg
     2281 gcgaacgttt gcctcggtct ccctgacgat cagcaggcca cgaaattcgt cgatccagcc
     2341 gtaatcgccg cccgtcgcaa cgagcttggg cgttgccaaa gtcctgatca attcgggaag
     2401 acgtcgctcg gaaaacgtcg tcaatagagg gcggacgtcg aagatttgcg tcgggggatc
     2461 ttcgcggtag tccgaagaga cgatctcgat cacttcgttg gcggtgcgaa agttgagacc
     2521 ccgctgcgcc agcatggccc gcagcacgtc gcgcgcaggt ccggccgtcg gatcgcagct
     2581 cacgggcaag cgcgaggcgg gttcttcatg tcggaccgac cgctcgtcga tcaccaccgg
     2641 cacacccagt tccgattgca gttggcgaca ggctgcctcg agcggcgtat tctccaggcg
     2701 cagggccagc ggccggtcca gtgcggcggc aatccgctgc tcgttttgct cggccaccgt
     2761 cagcggccac agcacgtcgg cttcggccgg cgacagcttg cggcgcagtc gcgtgagaaa
     2821 gtcggcgatt cgctcgtgaa cttccggaga ctgcggaatg accagccaat gatcgccgaa
     2881 ggcaaaatcg ggtgtgggac ccgaaaatcc ccacgactgc ggcgcgacgc aggtcgtgat
     2941 caaatcgact agaaacccga gagcgggttc gtccggcgtg gccaggtcgc caacgggata
     3001 agccacgagc acttcttgac agtcagcgtc gtcgggcgtg gaaatttgga ggaggccatt
     3061 ccgtacgaca tacgtcagat cgcacttgcg gagcagcagc cgcagcagcg accgaagcgt
     3121 gagtcccctc agctgcatcg tcacccgcat cgcgggatca atgccggcat cgtcaaggca
     3181 tttcaggttc agcgcgatcg gaatcgcgtg ccggcggctg agctcctgga ccacttcgca
     3241 cagcggccga tcgacgcagt cgagcgctga cggctcggcc agcgccgagt gaatccgcct
     3301 ttccagcgca ccaagcttgt ctggcagcag ccaaagcggc tccaaggatt gcggctgctc
     3361 gtcccgatcg gcgaatgcag agaacactgt tgcgatctgc cgctgaacct ccgtcgtgtg
     3421 ccgcaccacc aacccgccgg gaaccactgt caaatgcgcc ggtccgcccc tatcgtccca
     3481 agtgtgcggg gcaatcgtgt acttgatcag ctccacccat tgctccgcgt ccgcgctgga
     3541 ttcaagtggt ggcagcggat gaactgcaac caccagacgg tccgattggc ggtctgtttg
     3601 cagttcgcgc cacgccgcca gccggcgcgc ggctgtttgg cgctgctgat agaaatacga
     3661 gagtccagca attacggcga tactggccag cgacaccgcg acgagaagca ggcgggttct
     3721 catggccgaa ggactcggca gccgcgaacc ggctgcgcgg gtgtgcggtc ttgctggaag
     3781 agagtccgca cgtggcccca ggagaagcgt aggggagcgg gcgagggaaa gtcaagcgcg
     3841 cgatccaatg cggaatgcgg agtgcggaat gcggagttgg gaacagattg ccggtgagcc
     3901 cgcgctactc tgcagggttg ccgataggtg tacaggtgat ataatcaccc gtctgccagg
     3961 agcgctccgc tggccgtcca tatgaattcg caagcgcgca taatgcgcat aacggaaatc
     4021 gactagtttt cgccacaaca cctttcagaa taaacactta cagcaaaaac tgtcgtccgg
     4081 aaaaaatgtt tgcgatatgc gcgcatcacc ggtaccccct ggattaattc tgcactccga
     4141 actccgcatt ccgaatttcc ccatgtcccg catcgtcctt gccatgtccg gcggcgttga
     4201 ttcgagcgtc gccgcgcact tgctgaagga gcaggggcac gaggtgatcg gcgtgttcat
     4261 gcggcacggc gaggaggccc aggacgcgtg tgccgcggac gggccgccgg gatttaagct
     4321 gccgattttc gattcccggg cggaccacaa gcagggttgc tgctcggcca gcgacgccga
     4381 agacgcccgc cgcgtggccg accggctgga tattccgttt tacgccctga acctgcaggc
     4441 cgaatttcgt cggatcgtcg attacttcat cgacgaatac agccgcgggc ggacgcccaa
     4501 cccgtgcgtg cagtgcaaca actggctgaa gtttggcaag ctctttgact atgccgacag
     4561 cgtcggggcg gagtttgtgg ccacggggca ttatgcgcgg ctaagtagca ggcacactcc
     4621 gtgtgccgtc ctgctctccg ccggcaacgg c
//
LOCUS       DASRJB010000945         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2811285, whole genome shotgun sequence.
ACCESSION   DASRJB010000945 DASRJB010000000
VERSION     DASRJB010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811285"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1336)
                     /locus_tag="VFB80_25050"
     CDS             complement(236..1336)
                     /locus_tag="VFB80_25050"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN37133.1"
                     /translation="MSRISRRRVLGAMAAAGASLAWGRQAKSADVRDVAWLAEIQQRP
                     ANVPGDAPLLSDLKVASLAAWKAQRDELRRWWLEFLGPMPAKRKAVPKLEILEEDQKD
                     GVIRQLVRYVVEPADSMRPAEFTEAYLLKPAKLAGKARGIAVFHSTVEYSIRQPAGLE
                     GPPEKHFGLRYAKQGCVCFCPRNYLWPKTNGIAAAAEAEKYLKRVPGSKGMTKMLYDA
                     LVAVDILAALPEVDPERIGSVGHSLGAKETLYLAAFDERVKATVSSEGGVGTKFSNWD
                     AAWYLGGTIKEPTFTHEQHELLALAAPRPFLLVGGNSADGDRGWPFIAAALPIYELYG
                     KPARLGQFNHGKGHAVPPEAEAKIEEWLMTYL"
     gene            complement(1362..2135)
                     /locus_tag="VFB80_25055"
     CDS             complement(1362..2135)
                     /locus_tag="VFB80_25055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN37134.1"
                     /translation="MLKELFNLSGRAALVTGGSKGIGKAVARGLAECGANVAISARHE
                     DELKKAKAEIADGTGVKVEYFVADMNDRAAVKKLAADVLKTFGRCDILFNNAGSNKPQ
                     PLVETTDEVWDNIVELNLTSCMRLSREIAPSMIKNKWGRIIYTSSIMGLASNPARGCY
                     SATKAALIGMARAQALELGPHGITVNCLAPGPVATDLPMSILSPEQKKVFAERTAVKR
                     WGQAIDMVGPVLMLATDAGAFITGNVILADGGILCRTFD"
     gene            complement(2258..2635)
                     /locus_tag="VFB80_25060"
     CDS             complement(2258..2635)
                     /locus_tag="VFB80_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37135.1"
                     /translation="MNAAFYCMARRPAPLSEDEREAVRKLEAKYSVESRIKRFQETGQ
                     GIDWRAWRFLPPEQFSAADTILEGSAELPCESEESLATAIAHWCNFATGLRREVAKAL
                     WEVRVGDREIKHDYRNDRYDPAA"
     gene            complement(2669..>3042)
                     /locus_tag="VFB80_25065"
     CDS             complement(2669..>3042)
                     /locus_tag="VFB80_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN37136.1"
                     /translation="NPEYYDVIVTTNMFGDIITDLGAMIQGGLGVAAGGNINPDKGGV
                     SMFEPMGGSAPKYTGQNVINPIAAIAAMSMLLEHSGQPAAGARIMKAIQQVTGTKMKS
                     QSAGKMGYGTKEVGDLVVAAL"
BASE COUNT          464 a         1025 c          962 g          591 t
ORIGIN      
        1 ctggcgctct tcatcggcac gctcaaggtt gcccaggcgg cgctgcccgg tcctgtcgta
       61 atcctcgtcg ccctcgcggc cctgctcctc tggacgccgc aatacatgat cttcttcgtg
      121 tggcggatcg gtgaccactg aggaaaatct caagaaccga agacccaaag aaccaaagtg
      181 atcagcagac gccctgtctc ggatttggtt cttcggttct ttggttcttc ggttcttaga
      241 ggtacgtcat tagccactcc tcgatcttgg cttccgcctc cggcggcacg gcgtggcctt
      301 tgccgtggtt gaattggccc aggcgggcgg gcttgccata cagctcatag atcggcaagg
      361 ctgcggcgat gaacggccag ccgcgatcgc cgtcggccga attgccgccg accagcaaaa
      421 acggccgcgg cgcggccagg gcgagcagct cgtgctgctc gtgcgtgaac gtcggctcct
      481 tgatcgtgcc gcccagatac caggcggcgt cccagttgga gaacttcgtc ccgacgcccc
      541 cttcgctgct gaccgtggcc ttcacccgct cgtcgaaggc cgccaggtag agcgtctcct
      601 tggcccctag cgagtggccg acgctcccga tccgctccgg atcgacctcg ggcagcgccg
      661 ccaaaatatc gacggcgacc agcgcgtcgt agagcatttt ggtcatgccc ttggagcccg
      721 gcacgcgctt caggtacttc tcggcctccg ccgccgccgc aatgccgttg gtcttgggcc
      781 agagataatt ccgcgggcag aagcagacgc agccctgctt cgcataccgc agcccaaagt
      841 gcttttccgg cggaccttcc agacccgccg gctgccggat cgagtattcg accgtcgagt
      901 gaaagaccgc gataccccgc gccttgcctg ccagtttggc cggcttgagc aaataggctt
      961 cggtgaactc tgctggcctc atcgaatcag ccggttcgac gacgtaccgc acgagctgcc
     1021 gaatgacgcc gtccttttga tcttcctcca ggatctccag cttcggaacg gccttccgct
     1081 tggccggcat cggccccagg aactccagcc accagcggcg cagctcgtcg cgctgcgctt
     1141 tccatgccgc caacgacgca actttcaggt cggagagcag tggcgcgtcg ccgggtacgt
     1201 tcgccggccg ctgctgaatc tccgccagcc aggctacgtc ccgcacgtcc gccgacttgg
     1261 cctgtcggcc ccaggcgagg ctcgcgccgg cggcagccat cgctcccagt acccgccgcc
     1321 ttgagatccg cgacatggct tggctcccac atccgcaaga atcaatcgaa cgtccggcag
     1381 aggatgccgc cgtcggcgag gatgacgttt ccggtgataa atgcgccggc atcagtggcc
     1441 agcatcagga ccgggccgac catgtcgatc gcctggcccc agcgcttgac ggccgtccgc
     1501 tcggcaaaga ctttcttctg ctcgggcgag aggatgctca tcggcaggtc cgtggcgacc
     1561 ggccccggcg cgaggcagtt caccgtgatg ccgtgggggc ccagctccag cgcctgggcc
     1621 cgggccatgc cgatcagggc ggccttcgtc gccgaatagc agccgcgggc cgggttgctg
     1681 gccagcccca tgatcgatga cgtatagatg atccggcccc acttgttctt gatcatcgag
     1741 ggggcgatct cgcgggaaag ccgcatacag ctggtcaggt tcaactcgac gatgttgtcc
     1801 cagacctcgt cggtcgtctc gaccagcggc tgcggcttgt tgctgccggc gttgttgaac
     1861 aggatgtcgc agcggccgaa cgttttgagg acgtctgccg cgagcttctt cacggccgcc
     1921 cggtcgttca tgtcggcgac gaaatactcg accttcacgc ctgtcccatc ggcgatttcg
     1981 gccttggcct tcttcagctc gtcctcgtgg cgggccgaga tcgccacgtt cgcgccgcac
     2041 tcggccaggc cgcgggcgac cgccttgccg atgcctttgc tgccgccggt gaccaaggcc
     2101 gcgcggccgg agaggttgaa gagttctttg agcacgggaa gttgggggtt gggagtcggg
     2161 ggtcaggagt cggtacatgc ctagtacgga attgttgacc gtggcgtggc ccggtgcaat
     2221 ccgccgactc ccgactcccg acccctgact cccggttcta cgccgccggg tcgtagcggt
     2281 cgttgcgata gtcgtgctta atctcgcggt cgccgacgcg gacctcccag agggccttgg
     2341 cgacttcgcg gcgcaggccg gtggcgaagt tgcaccagtg ggcgatggcc gtggccagcg
     2401 attcctcgct ttcgcagggc aactcggcgc tgccttccaa gatcgtgtcg gcggccgaga
     2461 actgctcggg cggcaggaac cgccaggccc gccagtcgat gccctggccc gtctcctgga
     2521 accgcttaat gcggctttcg accgagtact tggcctccag cttgcgaacg gcctcgcgtt
     2581 cgtcctcgga aagcggcgca ggccgccgtg ccatgcaata aaaggcagca ttcacaccct
     2641 ggctccttgc ctggcgaacc ggccgcggtc acagcgccgc gacgacgagg tcgcccactt
     2701 ccttggtccc atagcccatc ttgccggccg attggctctt catcttggtg ccggtgactt
     2761 gctggatggc tttcatgatc cgcgcgccgg cggcgggctg gccgctgtgc tccaggagca
     2821 tgctcatggc ggcgatagcg gcgatcgggt tgatgacgtt ctggccggtg tacttggggg
     2881 cggagccgcc catcggctcg aacatgctca cgccgccctt gtccgggttg atgttgccgc
     2941 cggcggcgac gcccaggccg ccttgaatca tggcgcccag gtcggtgatg atgtcgccga
     3001 acatgttggt cgtcacgatc acgtcgtagt actcagggtt ct
//
LOCUS       DASRJB010000946         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_2813606, whole genome shotgun sequence.
ACCESSION   DASRJB010000946 DASRJB010000000
VERSION     DASRJB010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813606"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFB80_25070"
     CDS             <1..219
                     /locus_tag="VFB80_25070"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZN37137.1"
                     /translation="YTKLPTKKEADYERPFTLKRGGTLLDVAWLVHRDMAENFKYARV
                     WGSAVLSPGTQMKGDYVIHDKDVVEIHA"
     gene            complement(220..831)
                     /locus_tag="VFB80_25075"
     CDS             complement(220..831)
                     /locus_tag="VFB80_25075"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZN37138.1"
                     /translation="MSSVIAPTFGFAFPPLTPQPRRFSCAEFHQLGDMGWFEGRRAML
                     IDGEILEMPGPNPPHATMTAKVDYTLRRYFTQGFVIRNQSPLVLGQATDPEPDLAVVA
                     GKLMDFYSAHPTTAALVIEIADSTLAFDTTTKASLYAAAKIADYWVVDLVGKRLIVFR
                     QPQPDSAQPFKAKYASVQPLQPPGTISPLSAPVASIPVADLLP"
     gene            947..2680
                     /gene="polX"
                     /locus_tag="VFB80_25080"
     CDS             947..2680
                     /gene="polX"
                     /locus_tag="VFB80_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZN37139.1"
                     /translation="MTNAHIADAFDLIGDILDFQGANPFRVRAYRNSARTIRDYPEPL
                     AGMVETGKQKLVEIDGIGDDLAAKIITLVQTGSLPQLEELKKQVPDSVLALLRIPGVG
                     PKKAAALHKELKILTLEQLKAACEAGQVRELKGFGEKTEQLILQGIGIASQAGQRLYW
                     AEADQIVQELREHFRGCKSIKQLEFGGSYRRGKETIGDLDILVVAPDPTEAMDCLASF
                     PEATTVIGRGETKMSIRIASGFQVDLRVVPAESFGAALQYFTGSKEHNIILRGRAKDQ
                     GLKISEWGVFRVKGEKETRIAGATEQEVYDTLKLPVFPPEIREGRREFEWADAGKLPE
                     LITLEDIRGDLHMHTTASDGSASIEEMIAAARAQGLSYIAITDHSQRVSMARGLNGER
                     LLEQWQEIDKIRENLPKGFTLLKGIECDILEKGGMDLPDDVLAQGDWIIASIHYGQKQ
                     SRQQITERILGAIENPHVDIIAHPTGRLINRREAYEVDLDAVMAAAKKHGKLLELNAN
                     PARLDLNDVYCAAAKQHGIPIVINTDAHSPDGFEVLRYGILQARRAGLTKVDVANTRP
                     WPQLKKLIGKP"
BASE COUNT          611 a          933 c          881 g          434 t
ORIGIN      
        1 tacaccaagc tgccgacgaa gaaagaggcc gactacgagc ggccgttcac gctcaagcgc
       61 ggcggcacgc tgctcgacgt cgcctggctt gtgcaccgcg acatggccga gaacttcaaa
      121 tacgcccgcg tctggggcag cgccgtcctc tcgcccggca cgcagatgaa gggcgactat
      181 gtcatccacg acaaggacgt ggtggagatt cacgcctgac tatggcagca agtcggccac
      241 gggaatcgaa gccaccggcg ccgacagcgg cgaaatcgtc cccggcggct gcagcggctg
      301 caccgatgcg tatttcgcct tgaatggctg cgccgaatca ggctgaggct ggcggaatac
      361 gattagccgc ttgccgacca gatcgacgac ccagtaatcg gcgatcttgg ccgcggcata
      421 gaggctggcc ttggtcgtgg tgtcaaaggc cagcgtgctg tcggcgattt cgattaccag
      481 tgccgccgtg gtcggatgag ccgagtaaaa atccatcaac ttcccggcca ccacggccag
      541 gtctggctcc gggtccgtcg cctggccgag caccagcggc gactggttgc ggatgacaaa
      601 accctgcgtg aaatagcggc ggagcgtgta atcgaccttg gccgtcatcg tggcgtgcgg
      661 cggattcgga ccgggcattt ccagaatctc cccgtcgatc aacatcgccc gccggccctc
      721 gaaccagccc atgtcgccca gctgatggaa ctcggcgcaa gaaaaccgcc gcggctgagg
      781 agtcagcggc ggaaacgcga agccgaaggt tggagcgatg acggaagaca tggcaatcaa
      841 cggcggctga ttcacgatgc agctttatcc atcataatcg acgttagcaa cgcaggccaa
      901 acgcgcacgc aatctcgaac cttcaacttg gaacttgaac tgccccatga ccaacgccca
      961 cattgccgac gccttcgatc tgatcggcga catcctggac tttcagggcg ccaacccgtt
     1021 ccgcgtgcgg gcttatcgca acagcgcccg cacgatccgg gattatccgg agccgctggc
     1081 cggcatggtc gaaacgggaa agcagaagct ggtcgagatc gacggcatcg gcgacgacct
     1141 ggcggccaag atcatcacgc tggtccagac cggctcgctg ccgcagctcg aagagctgaa
     1201 gaaacaggtg cccgacagcg tgctggcact gctccgcatc cccggcgtcg ggccgaagaa
     1261 agccgcggcc ctgcacaagg agctgaaaat cctcacgctc gagcagctca aggcggcctg
     1321 cgaagccggc caggtccgcg agctgaaagg ttttggcgaa aagaccgagc agctcatcct
     1381 gcagggcatc ggcatcgcct cgcaggccgg ccagcggctg tactgggccg aggccgacca
     1441 gatcgtgcag gagctgcgcg agcactttcg cggctgcaag tcgatcaagc agctcgaatt
     1501 cggcggcagc tatcgccgcg gcaaagagac gatcggcgac ctcgacatcc tcgtcgtcgc
     1561 cccggacccg acggaggcga tggactgcct cgcgtcgttt cccgaggcga ccaccgtaat
     1621 cggccgcggg gagaccaaaa tgtcgatccg catcgcgagc ggcttccagg tcgatctgcg
     1681 cgtcgtccct gccgagtcgt tcggcgcggc cctgcagtac ttcacgggct ccaaggagca
     1741 caacatcatc ctccgcggcc gggcgaaaga tcaggggctg aagatcagcg agtggggcgt
     1801 gttccgcgtc aaaggagaga aggaaacgcg gatcgccggc gcgaccgagc aggaagtcta
     1861 cgacacgctg aagctgccgg tctttccgcc ggaaatccgc gaggggcggc gcgagttcga
     1921 gtgggccgac gccggcaaac tgccggagct gatcacgctg gaggacatcc gcggcgacct
     1981 gcacatgcac acgaccgcct ccgacggcag cgcctcgatc gaagagatga tcgccgccgc
     2041 ccgcgcgcag ggcctgtcgt acatcgccat caccgatcac tcgcaacgcg tcagcatggc
     2101 ccgcggctta aacggcgagc ggctgctcga gcagtggcag gagatcgaca agattcgcga
     2161 gaacctgccc aaaggcttca cgctcctcaa agggatcgag tgcgacatcc tggaaaaggg
     2221 cggcatggac ctgcccgacg acgtgctcgc ccagggcgac tggatcatcg ccagcatcca
     2281 ctacggccag aagcaatcgc ggcagcagat caccgagcga atcctgggcg cgatcgagaa
     2341 tccgcacgtc gatatcatcg cgcacccgac cggccggctg atcaatcgcc gcgaagccta
     2401 cgaggtcgat ctcgacgcgg tgatggccgc cgccaagaag cacggcaagc tgctggagct
     2461 caacgccaac ccggcccggc tcgatctcaa cgacgtctac tgcgccgccg cgaagcagca
     2521 cggcattccc atcgtcatca acaccgacgc ccactcgccc gacggcttcg aggtcctccg
     2581 ctacggcatc ctccaggccc gccgcgccgg cctcaccaaa gtcgacgtcg ccaacacccg
     2641 cccgtggcca cagctcaaaa agctgatcgg gaaaccttga atcgacttcc ggcagaaacg
     2701 cgaaagcgcg aaagaacgaa gcgcgaaaca gagcagaact aagctagatc gactctccgt
     2761 tctcattcgc gttttcgcgc tcccgtatcc ttacaacatt ggagttttgc agcatgggtc
     2821 atgcatttga gatgttgtca gggcagatta tcgaggcgg
//
LOCUS       DASRJB010000947         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4224201, whole genome shotgun sequence.
ACCESSION   DASRJB010000947 DASRJB010000000
VERSION     DASRJB010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224201"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFB80_25085"
     CDS             complement(<1..514)
                     /locus_tag="VFB80_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN37140.1"
                     /translation="MKLGLINSAWVQANQPTVYGLRKTKELGFDSVDIFVDPLDIDVR
                     ERRLIKNECDRLELPIVSIACVAVGLIDFNPSVQRFHIDRVRKYLDLAFEYEAKNVLL
                     VLGEYIWNQEVIPPAEQWKTGVENCRRLGDYAAGLGVKIALELEPFHLSLVNDVDSMA
                     RFLDDVGHPAV"
     gene            666..1397
                     /locus_tag="VFB80_25090"
     CDS             666..1397
                     /locus_tag="VFB80_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HZN37141.1"
                     /translation="MTSTAGDPQFHTTHWSLIAAATGDGGEQAQAALEEVCRAYWYPV
                     YAFIRRRGQPAEDAGDLTQAFFATLLEKEYLADADRERGRFRAFLLTAVARFVAKERD
                     KAAAQKRGGGRQLFPIDLADGEARYRHEPAHDWTAERVFERRWALTLLDRTLARLRQE
                     HATAGKQALFDALKVYLTGEAGAPPLRHVAGQLQMTEGAVKVAVHRLREKYREALRAE
                     VAQTVAAEGDVDDELGLLLAALQGR"
     gene            1489..>2776
                     /locus_tag="VFB80_25095"
     CDS             1489..>2776
                     /locus_tag="VFB80_25095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN37142.1"
                     /translation="MMGLGWSELLLLVLCGGAFLIVPTVVVLIIVAAQRRPAEAPAAA
                     WRQAPPPIAPLSPARGPDDADARHTIIALLTQRFCPQCRAPLAADAPEGLCPACLMAG
                     AIGTRAADAADRGLAATTPPSGSQPPAAGEWSNLQQHFPDLEILELLGRGGMGSVYKA
                     RQRNLDRLVALKVVPPEAAKDPAFTERFNREGRALARLNHPNIVTVYDFDQKGDVYFL
                     LMEYVDGVNLRHALRASRLAPQEALAIVPQICDALQYAHDQGVVHRDIKPENVLLDRS
                     GRVKIADFGLAKLLGKGPDDFTLTRTQQVMGTPRYMAPEQIERPSTVDHRADIYSLGV
                     VIYEMLTGELPLGRFEPPSHKVQLDVRIDQVVLRTLEKEPDRRYQRASHLKTELASAA
                     TWQTPAPAAQPSTPPPQQPSWHTPAPASKPAASDPGG"
BASE COUNT          459 a          949 c          928 g          440 t
ORIGIN      
        1 tcacggccgg gtggccgacg tcgtcgagga accgggccat gctgtccacg tcgttgacca
       61 gggacaggtg gaacggctcc agctccaggg cgatcttcac gcccagcccc gcggcgtaat
      121 cgcccagccg gcgacagttc tcgacgccgg tcttccactg ctcggcggga ggaatgactt
      181 cctgattcca gatgtattcg ccgaggacga gcagcacgtt cttggcctcg tactcgaaag
      241 ccaggtcgag atacttcctc acccggtcga tatgaaaccg ctgcacgctg gggttgaagt
      301 cgatcaggcc gacggccacg caggcgatcg agacgatcgg cagctcgaga cggtcgcact
      361 cgttcttgat cagccgccgc tcgcggacgt cgatgtcgag tggatcgacg aagatgtcca
      421 ccgaatcgaa gcccagttcc ttggtcttcc gcagaccata aaccgtcggc tgattggctt
      481 gcacccaggc ggagttgatg agtccgagtt tcatggctcg tctgcggtta gggacgactt
      541 ctgcttagcc ccggcgtcca cgccggtcgg cgggtgtggc acggtcggga gaccggccac
      601 aacgatgggg tcgggagagc gtcgacaacg atgctgaaag cataacgcgg tctatactgg
      661 accgcatgac ctccacggcg ggcgatccgc aattccacac gacgcactgg agcctgatcg
      721 ccgcggcgac cggcgacggg ggcgagcagg cgcaagcggc gctggaagag gtctgccggg
      781 cttactggta tccggtgtac gcgttcattc gccgccgcgg ccagccggcc gaggacgccg
      841 gcgatctgac ccaggccttc ttcgccacgc tgctggagaa ggagtatctc gccgacgccg
      901 accgcgagcg cggccggttt cgagcgttcc tgctgacggc cgtggcccgg ttcgtcgcca
      961 aagagcgcga caaggccgcg gcccagaaac gcggcggcgg ccggcaactc ttcccgatcg
     1021 acctggccga cggcgaagcc cgctaccgac acgaacccgc gcacgattgg actgccgaac
     1081 gcgtcttcga gcgccgctgg gccctgacgc tcctggaccg cacgctggcc cgcctccgcc
     1141 aggagcacgc caccgccggc aaacaggcct tgttcgacgc gctgaaggtt tacctgacgg
     1201 gcgaggccgg cgcgccgccc ttgcggcacg tcgccggcca gctgcagatg accgagggag
     1261 cggtcaaggt cgccgtgcat cgcctgcgcg agaaatatcg cgaggccctg cgggcggaag
     1321 tagcgcaaac cgttgccgcg gaaggagatg tggacgacga gctgggcttg ctcctggccg
     1381 ctctgcaagg acgctaggcc gcttcggcgt aaccatcgcc cgtttttgcg taagtcctgg
     1441 gtgactggtt acgattctgt cacagcggtc atccgcggca ggtgaaacat gatgggcttg
     1501 ggctggtcgg aattgttgtt gctcgtgctc tgcggcgggg cctttttgat cgtgcccacc
     1561 gtcgtggtgt tgatcatcgt ggcggctcag cgccggccgg ccgaagcgcc agctgcggcc
     1621 tggcggcagg ctccgccgcc aatcgcacct ctctctccgg cccgcggtcc agacgatgcc
     1681 gacgcccgcc acacgatcat cgccctgctg acgcagcggt tctgcccgca gtgccgcgcg
     1741 ccgctggcgg ccgacgcgcc cgaggggctc tgcccggcgt gcttgatggc cggggcgatc
     1801 ggaacccggg cggctgatgc agccgaccgc gggctggcgg caaccacgcc cccttcgggc
     1861 tcgcagccgc cggccgccgg cgagtggtcc aatctgcagc agcattttcc cgacctggaa
     1921 atcctggagc tgctcggccg cggcggcatg ggctcggtct acaaggcccg gcagcgaaac
     1981 ctggatcggc tggtggcctt gaaggtcgtg ccgcctgagg cggcgaagga cccggccttc
     2041 accgaacgct ttaaccgcga ggggcgggcc ctggcccgtc tcaatcatcc caacatcgtc
     2101 acggtctatg acttcgacca aaagggcgac gtctattttc tgctgatgga atatgtcgat
     2161 ggggtcaacc tgcggcacgc gctgcgggct agccgactcg cgccgcagga agccctggcg
     2221 atcgtcccgc agatctgcga cgcgttgcaa tacgcccacg accagggcgt ggtgcaccgc
     2281 gacatcaagc ccgagaacgt gctgctcgac cgctcgggcc gggtgaagat cgccgacttc
     2341 ggcctggcca agctgctcgg caaaggcccc gacgacttca cgctgacccg cacgcagcag
     2401 gtgatgggca cgccgcgcta tatggctccc gagcagatcg agcggcccag caccgtcgat
     2461 caccgggccg acatctattc gctcggcgtc gtgatttacg agatgctcac gggcgagctg
     2521 ccgctgggcc gcttcgagcc gccctcgcac aaagtgcagc tcgacgtgcg gatcgaccag
     2581 gtcgtcctgc ggacgctcga gaaggagccc gaccggcgct atcagcgggc cagccacctc
     2641 aagaccgaat tggcctcggc tgccacctgg cagacgccgg ctcccgccgc gcaacccagc
     2701 acgccgccgc cgcagcagcc cagctggcac acgccggcgc cggcgagtaa gccggccgcg
     2761 tccgatcccg gcggcg
//
LOCUS       DASRJB010000948         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_4229007, whole genome shotgun sequence.
ACCESSION   DASRJB010000948 DASRJB010000000
VERSION     DASRJB010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229007"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1538
                     /locus_tag="VFB80_25100"
     CDS             237..1538
                     /locus_tag="VFB80_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN37143.1"
                     /translation="MAFSLTCDGVKRRDFLKAGALGMGLSLSGYLRLAAAGEIGPAKA
                     KSAIFINLTGGPSHMDSFDLKPDAPAEYRGQFNPIKTNAPGVEISEHLPNLAKCADKF
                     AILRGVSHTLGAHELGTEYVNTGSRPIPSLEYPGYGAVVTKELGGPKDLPPFVAIPNS
                     NQRPGFLGVQYAPLNTTSAPRPGVPYGVRGITLGNGLTVEEVERRTNLLADLDKTFAS
                     VESNSQLLTGLDRFSEQAHAIITSKKARDAFDVSKETPAYAKPFGEDPFGMSCLLASR
                     LVESGVRFVTLTLGGWDTHNSNFTRLKTNLLPKLDVGLAALLNGLAEKGLLDSTSVFV
                     TGEFGRTPKINSRDAEGGRDHYPRCMFMVLAGGGVRGGQVLGESDDKATMPKNEGFSP
                     DDVAATFYHTLGIDHTKEYQTNSGRPITIVRDGKVIRGLFS"
     gene            1651..2139
                     /locus_tag="VFB80_25105"
     CDS             1651..2139
                     /locus_tag="VFB80_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN37144.1"
                     /translation="MKSLAPKLLVVFCLLAAVMTWSSAVGQEAKKRKADPSAQIKKKL
                     EEAVLQPDLLEKCNKIVAEHAAKIAEATAKVNAIYTAEQLQAKRTTQKAAKDAGKKGR
                     EAAAEVEAAMKLTDEQKTKLEAANKELTAATNVRNDALRAVLSAEQAEKVLGKAKKRK
                     NA"
     gene            complement(2208..>4509)
                     /locus_tag="VFB80_25110"
     CDS             complement(2208..>4509)
                     /locus_tag="VFB80_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN37145.1"
                     /translation="ALGCYWRKDGVPIWQNPQLIAEDGSDYRAGPEQAHQFAATLAKK
                     LGVDPKFAIPGHEDAWYYLWRERRLPTNVDPLESRLEDPEERARLAKVFEQGLNTIVG
                     YALPIQRVHTGAEAYWLSGQWFLRAGHMYLTPGDSPMGYRLPLDSLPWAAPEHSLQPL
                     ALRDPFDRPGPLPRLQSGGRQPPEVERTVRLQSPTAESQAAAGSVGSRPPLAEFASRP
                     PLAGQSQPGVVRTALCVEPRGGTLRIFMPPLGHFEDYLELVQAIEATALELALPVQIE
                     GYTPPFDTRVNHFKITPDPGVIEVNIHPANQWDELSDHTRILYEEARQSRLCTEKFML
                     DGRHTGTGGGNHVVLGGPTPGDSPLLRRPDLLRSLVAYWHNHPSLSYLFSGMFVGPTS
                     QAPRVDEARNDSLYELEIAFREIPEYGDVRPWLVDRVFRHLLVDLTGNTHRAEFCIDK
                     LYSPDSADGRRGLLEFRAFEMPPHWQMSLVQQMLLRALVARFWEQPYRAKLVRWGTEI
                     HDRFMLPHFVQQDFQDVLFELRQSGLAFQEAWFAPHFEFRFPILGQVAQRGIHVELRQ
                     AVEPWHVLGEEAAGSATARYVDSSVERLQVKVTGLTDTRHVVACNGRRIPLHPTGTSG
                     EFVAGVRYRAWQPPSCLHPTIGVHAPLVMDILDTWLGRSLGGCTYHVAHPGGLAHDTF
                     PVNANEAEGRRVARFFAFGHTPGPVVIPPEIRSPEAPLTLDLRLPPAEADVLSRMLPA
                     SADTYRGNGQTLQPHARLVEREHSYS"
BASE COUNT          827 a         1469 c         1514 g          699 t
ORIGIN      
        1 tgccctggtt cgtcaggctt acctgcggac gctcagccgg ctgccgacgc cggacgagat
       61 caaccgctgc tcgcaatacc tggcccaggc cgaatcgccg cttgccggcg ccaagggcct
      121 gctctggacg ctcattaaca ccaaggagtt catcgttaac cactaacgtt cgacggagcg
      181 attgacgttc gcattttcca acagacaccg ttccattttc agaagggatc cgacttatgg
      241 cgttctctct tacttgtgat ggcgtgaagc gccgcgactt cttgaaggca ggcgccttgg
      301 gcatgggcct ctcgctgtcc ggctacttgc ggctggctgc ggccggcgag atcggcccgg
      361 cgaaggccaa gagcgcgatc ttcatcaacc tgacgggcgg cccgtcgcac atggattcgt
      421 tcgacctcaa gccggacgcc cccgcggaat accgcgggca gttcaatccg atcaaaacga
      481 acgctccggg ggtcgagatc agcgagcacc tgccgaacct ggccaagtgc gccgacaagt
      541 tcgcgatcct ccgcggcgtc agccacacgc tcggcgctca cgagctgggc accgaatacg
      601 tcaacaccgg cagccggccg attccgtcgc tggaatatcc cggctacggc gcggtggtga
      661 ccaaggaact cggcggaccg aaggacctgc cgcccttcgt ggcgattccc aacagcaacc
      721 agcggcccgg cttcctgggc gtgcaatatg ccccgctgaa cacgaccagc gcgccgcgcc
      781 cgggcgtgcc ctacggcgtc cgcgggatta cgctgggcaa cggcctgacc gtggaagagg
      841 tcgagcggcg gacgaacctg ctggccgacc tggacaagac cttcgcttcg gtcgagtcga
      901 acagccagct gctgaccggc ctcgaccgct tcagcgagca ggcccacgcg atcatcacca
      961 gcaagaaggc ccgcgacgcc ttcgatgtga gcaaggaaac gccggcctat gccaagccgt
     1021 ttggcgagga tccgttcggg atgagctgct tgctcgcctc gcggctggtc gagtcgggcg
     1081 tgcggttcgt cacgctgacg ctcggcggct gggacaccca caacagcaac ttcacccgcc
     1141 tgaagacgaa cctgctgccc aagctcgacg tcggcctggc ggccctgctg aacggcctgg
     1201 ccgagaaggg gctgctcgat tcgacgagcg tcttcgtcac cggcgaattc ggccgcacgc
     1261 cgaaaatcaa cagccgcgac gccgagggcg gccgcgacca ctatccgcgg tgcatgttca
     1321 tggtgctggc gggcggcggc gtccgcggcg gccaggtgct cggcgagagc gacgacaagg
     1381 ccacgatgcc caagaacgaa ggcttctcgc ccgacgacgt ggcggcgacc ttctatcaca
     1441 cgctgggcat cgaccacacc aaggaatacc agaccaactc cggccggccg atcacgatcg
     1501 tccgcgacgg caaggtgatc cgcgggctgt tctcgtagcg cactgccgcc tgaccgacca
     1561 acttcgtgcc cacgccgacg cgcgtgggca cgtctgtttc ccgacgattc gaagacccat
     1621 tttccattca ttgttccgga gactctcccc atgaaatcgc tcgctccgaa gttgctggtt
     1681 gtgttctgcc tgctggccgc cgtaatgacc tggagctcgg cagtcggcca ggaagccaaa
     1741 aagcggaagg ccgatccgtc ggcccagatc aagaagaagc tcgaagaggc cgttttgcag
     1801 ccggacctgc tcgagaagtg caacaagatc gtcgccgaac acgcggcgaa gatcgccgag
     1861 gccacggcca aggtcaacgc gatctacacg gctgagcagc tacaggcgaa gagaaccacc
     1921 cagaaggccg ccaaggacgc cggcaaaaag ggccgcgagg cagccgccga ggtcgaagcc
     1981 gcgatgaagc tcaccgacga gcagaagacg aagctcgaag cggcaaacaa ggagttgacc
     2041 gccgcgacca acgttcgcaa cgacgctctc cgcgccgtcc tcagcgccga gcaggccgag
     2101 aaggtcctcg gcaaggcaaa gaagcggaag aacgcctagc ggtaatggcg ccgcgaattg
     2161 cgggagcagc tggaagccgc tgcggcgctc ttgagccgta atccgcgcta cgaataactg
     2221 tgctcgcgct cgacgaggcg ggcgtgaggc tggagcgtct gcccgttgcc gcgatacgtg
     2281 tcggcgctgg cggggagcat tcgcgagagg acgtcggcct cggccggcgg cagccgcagg
     2341 tcgagcgtca gcggagcttc gggcgagcga atttcgggcg gaatgacgac ggggccgggt
     2401 gtgtggccga acgcgaagaa gcgggccacg cggcggccct cggcttcgtt ggcgttgacg
     2461 gggaaggtgt cgtgagctag cccgccggga tgggccacgt gatacgtgca gccgccgagc
     2521 gaacggccca gccaggtatc gagaatgtcc atcactagcg gcgcgtggac gccgatcgtc
     2581 ggatgcaggc acgacggcgg ctgccaggcc cggtaacgca cgccggcgac gaactcgccg
     2641 ctcgtgcccg tcggatgcag cggaatccgg cggccattgc aggcgaccac gtgccgcgtg
     2701 tcggtcaggc cggtgacttt tacttgcagt cgttcgaccg acgaatcgac atagcgggcg
     2761 gtggcgctgc cggcggcctc ttcgcccagc acgtgccagg gctcgaccgc ctgccgcaat
     2821 tcgacgtgaa tgccgcgctg cgcgacctgg cccaggatcg ggaagcggaa ttcaaaatgc
     2881 ggcgcgaacc aggcttcctg gaaggccagg ccggactgcc gcagctcgaa cagcacgtcc
     2941 tggaagtcct gctgcacgaa atgaggcagc atgaaccggt cgtggatctc ggtgccccag
     3001 cggaccagct tcgcccggta gggctgctcc caaaagcggg ccaccagcgc ccgcagcagc
     3061 atctgctgca cgaggctcat ctgccaatgc ggcggcattt cgaacgcgcg gaactccagg
     3121 aggccgcgcc gcccgtcggc cgaatcgggc gagtagagct tgtcgatgca aaactcggcc
     3181 cggtgcgtgt tgccggtcaa atcgaccagc agatgccgga agacgcgatc gacgagccac
     3241 ggccgcacgt cgccgtattc gggaatctcg cggaaggcga tttccagctc gtacaggctg
     3301 tcgttgcggg cctcatcgac gcgcggggcc tggctcgtcg ggccgacgaa cattcccgag
     3361 aacaggtatg acagcgaggg gtggttgtgc cagtaggcga cgaggctccg cagcaggtcc
     3421 ggccgccgca ggagcgggct gtcgccgggc gttggcccgc cgaggacgac gtggttgccg
     3481 ccgcccgtcc cggtgtgacg gccgtcgagc atgaatttct cggtgcacag ccgggattgc
     3541 cgggcctctt cgtagaggat tcgcgtgtgg tcggacagct cgtcccactg attcgccgga
     3601 tggatgttga cctcgatcac gccgggatcg ggcgtgatct tgaaatggtt gactcgggtg
     3661 tcgaagggcg gcgtgtagcc ttcgatttgc acgggcagag ccagctccag ggctgtcgcc
     3721 tcgattgcct gcacgagctc cagatagtcc tcgaaatggc ccagcggcgg catgaagatc
     3781 cgcagcgtcc cgccccgcgg ctcgacgcag agtgccgtgc ggacgactcc cggctgcgat
     3841 tggccggcga gcggcgggcg tgacgcaaat tcggcgagcg gtgggcgtga gcccactgag
     3901 cccgcggcgg cctgagattc ggcggttgga gactgtagtc gcacagttcg ttcgacctca
     3961 gggggctgac gccccccgct ctgcaggcgc ggcagcgggc ccggccgatc gaacggatcg
     4021 cgcagggcga gcggctgcaa agaatgctcc ggcgcggccc agggcaagct gtccagcggc
     4081 aggcgataac ccatcggcga atcgccggga gtcaaataca tgtgcccagc gcggaggaac
     4141 cattggccgc tgagccagta cgcttccgcg ccggtgtgga cgcgctggat cggcagcgcg
     4201 tagccgacaa tcgtgttcag accctgctcg aagactttcg ccaaacgcgc ccgctcttca
     4261 gggtcttcga gcctgctctc gagcggatcg acgttggtgg gcagccgtcg ctcgcgccac
     4321 aagtaatacc aggcgtcttc gtggccgggg atcgcgaact tgggatcgac gccgagcttc
     4381 ttagccaacg tcgccgcaaa ttggtgcgcc tgctcgggac cggcgcgata gtcgctgccg
     4441 tcctcggcga tcagctgcgg attctgccag atcggcacgc cgtccttccg ccagtagcag
     4501 cccagggcc
//
LOCUS       DASRJB010000949         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U10125
            k141_1691575, whole genome shotgun sequence.
ACCESSION   DASRJB010000949 DASRJB010000000
VERSION     DASRJB010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963653
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:55:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,024
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691575"
                     /isolate="SMAG_U10125"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFB80_25115"
     CDS             complement(<1..677)
                     /locus_tag="VFB80_25115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN37146.1"
                     /translation="MFLAEPPRTQFDLNFQVLGFPVRVHPLFWLIGALIGYAFIRDYG
                     AIGLVIWVFAMFVSILLHELGHALMIRRFGRDAHIVLYAMGGLAIEGRPQSNYGNPWS
                     LDSYTGYQPRQRTPQEQILISAAGPGIQLVLAGLVIAAVYASGGYMRVGGGLLPLPQA
                     ILGRDLAANVNIQLLVHDLLFINIFWAIMNLLPVLPLDGGQIALQVFMQHDPWQGTIR
                     ALWLSVIT"
     gene            complement(736..1959)
                     /gene="lpxB"
                     /locus_tag="VFB80_25120"
     CDS             complement(736..1959)
                     /gene="lpxB"
                     /locus_tag="VFB80_25120"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZN37147.1"
                     /translation="MRIFFSVGEPSGDLHGANLIGELRRRGITDFAGLGGPRMKAAGC
                     DLLRDMSDLAVMGLVPALVKLPQFFRLLAQVERSWEASRPDAVVLIDYPGFNWHVARK
                     AKERGIPVFYYGLPQLWAWLSWRVKKVQRYVDHALCKLPFEEAWFRARGCRATYVGHP
                     YFDELRAQQLDEEFLRQCRARPERLLTILPGSRTHEVRSNLPMLLAAAARVRSALPGV
                     RLAIASYNEKQAVLARQLAGSGPAPAEIHVGRTPELIAAADCCFAVSGSVSLELLYHA
                     RPSVIVYRTSPWLYALLRRLVHVRYITLVNLLACDDPLATTGGRQPYEAGAPGHEQVL
                     FPEYPTWQDRSEAVAAHAIRWLSDEQERQRLIGKLKVLRERLAAGGASRAAADYIVQA
                     LAVDAEQGVTLRRAS"
BASE COUNT          440 a          783 c          741 g          321 t
ORIGIN      
        1 gtgatcaccg acagccacag cgcccggatc gtgccctgcc acggatcgtg ctgcatgaag
       61 acttgcagcg cgatctgccc gccgtcgagc ggcagcaccg gcagcagatt catgatcgcc
      121 caaaagatgt tgatgaagag caggtcgtgc accagcagtt ggatgttgac gttggccgcc
      181 aggtcgcggc ccagtatcgc ctgaggcaac ggcaagaggc cgccgccgac ccgcatatag
      241 ccgcccgacg catagaccgc cgcgatgacc agcccggcga gcaccagctg aatccccggc
      301 cccgccgccg aaatcagaat ctgttcctgc ggcgtgcgct gccgcggctg atagcccgtg
      361 tacgaatcga gcgaccaggg gttgccgtag ttcgattgcg gccggccctc gatcgccagg
      421 ccgcccatcg cgtagagcac gatgtgcgca tcgcggccga agcggcggat catcagcgcg
      481 tggcccagct cgtggagcag gatcgagacg aacatcgcga acacccagat caccaggccg
      541 atcgcgccat agtctctaat gaaggcgtag ccaatcagcg ccccgatcag ccagaacagc
      601 gggtggacgc gcacggggaa gcccaaaacc tggaaattca ggtcgaattg cgtccgcggc
      661 ggctcagcca gaaacacggg tcggctcctc aaaacaggca attatagccc gccgtagacg
      721 acgcggcctg cgtcgtcacg aggcccgccg cagcgtcacg ccctgctcgg catcgaccgc
      781 cagcgcttgc acgatgtaat ccgccgctgc ccgcgaagcg ccgccggctg ccaaccgctc
      841 gcgaaggacc ttcagcttgc cgatcagccg ctgccgctct tgctcgtcgc tgagccagcg
      901 gatcgcgtgc gcggcgaccg cctcggagcg gtcctgccag gtagggtact cggggaacag
      961 cacctgctcg tgccccggcg caccggcctc gtacggctga cggccgccgg tcgtcgcgag
     1021 cggatcgtcg caggcgagca ggttcaccag cgtgatgtat cgcacgtgca ccagccgccg
     1081 cagcagggca tagagccagg gcgaggtgcg atagacgatc acgctcgggc gggcgtgata
     1141 gagcagctcc agcgagacgg agccggagac ggcgaagcag caatcggcgg cggcgatcag
     1201 ctctggagtg cggccgacgt ggatctcggc cggcgcggga ccgctgccgg ccagctgccg
     1261 cgccagcacc gcctgcttct cgttatagct ggcgatcgcc agccgcacgc cgggaagagc
     1321 gctccgcacg cgggccgccg ccgccagcag catcggcaag ttgctccgca cctcgtgcgt
     1381 ccgcgaaccc ggcaggatgg tcagcagccg ctcgggccgc gcgcggcact gccgcagaaa
     1441 ctcttcgtcg agctgctgcg cccgcagctc gtcgaaatag ggatggccga cgtacgtcgc
     1501 ccggcagccg cgggcgcgga accaggcctc ctcgaacggc agcttgcaga gggcgtgatc
     1561 gacgtagcgc tggactttct tgacccgcca cgacagccag gcccagagct gcggcaggcc
     1621 gtaatagaaa acgggaatcc cgcgctcttt cgcctttcgg gccacgtgcc agttgaagcc
     1681 cggataatcg atcagcacca ccgcgtcggg ccgcgacgcc tcccacgagc gctcgacctg
     1741 cgccagcagt cggaaaaact gcggcagctt caccagcgcc ggcaccaggc ccatcacggc
     1801 caggtcgctc atgtcgcgta agaggtcgca gccggcggcc ttcatccgcg gcccgcccag
     1861 gccggcgaag tccgttatcc cccgccggcg caattcgccg atcaggttcg cgccgtgcaa
     1921 atcgccgctc ggctcgccga cggagaagaa gatgcgcatg gctgttcgtc cctgaacgcc
     1981 gctggatggg ccgtgccagt atcgcagacc cgcgaatgcg gccctagacc aacccgaagc
     2041 gtaagcgagg gactctgcgg acctccccag ccggctgcat gcagtttcac tgcaaaaagg
     2101 aggacggagt ttcccacccg cgtcctctac acaagaaaaa agagagcttc cttcgccaag
     2161 gcttgaaaaa ccagtgcggg tacagttcac tgggtcggga tttctgttga acagatgacg
     2221 aaggagcggg gtgcaatggc gagttcaacc agacgcagaa tcgggcgaaa ggcatcgtgc
     2281 gcggc
//
